bluuebunny/crossref_metadata_2025_split huggingface dataset:
The anti‐cancer effects of (−)‐Epigalocathine‐3‐gallate on the signaling pathways associated with membrane receptors in MCF‐7 cells
Authors: Hsu Yuan‐Chang , Liou Ying‐Ming 
Published: December 2010

Abstract(−)‐Epigallocatechin‐3‐gallate (EGCg) has been implicated in cancer chemo‐prevention in studies using many different kinds of cancer cells. The present study measured cell viability, osteopontin (OPN) secretion, fatty acid synthase (FAS) expression, and cytosolic Ca2+ and verified the anti‐cancer activities of EGCg in MCF‐7 human breast cancer cells. EGCg‐induced apoptosis was evidenced by nuclear condensation, increased protein levels of activated caspase‐3, down‐regulation of gelsolin and tropomyosin‐4 (Tm‐4), and up‐regulation of tropomyosin‐1(Tm‐1). By disrupting adherens junction formation, EGCg caused accumulation of extra‐nuclear β‐catenin aggregates in the cytosol and alterations of the protein content and mRNA expression of E‐cadherin and β‐catenin, but not N‐cadherin, in MCF‐7 cells. To identify the putative mechanisms underlying the EGCg signaling pathways, EGFP (enhanced green fluorescence protein) was ectopically expressed in MCF‐7 cells. This allowed us to monitor the EGCg‐induced fluorescence changes associated with the effects of Triton X‐100 (to remove plasma membrane) or the addition of laminin, anti‐laminin receptor (LR) antibody, epidermal growth factor (EGF), and genistein on the cells. Our results indicated that EGCg acts via the signaling pathways associated with cell membrane to suppress cell proliferation, provoke apoptosis, and disturb cell–cell adhesion in MCF‐7 cells. The altered events include the EGFR, LR, FAS, intracellular Ca2+, OPN secretion, caspace‐3, gelsolin, Tm‐4, Tm‐1, and adherens junction proteins, E‐cadherin and β‐catenin. J. Cell. Physiol. 226: 2721–2730, 2011. © 2010 Wiley‐Liss, Inc.
Role of epimorphin in bile duct formation of rat liver epithelial stem‐like cells: Involvement of small G protein RhoA and C/EBPβ
Authors: Jia Yali , Yao Hailei , Zhou Junnian , Chen Lin , Zeng Quan , Yuan Hongfeng , Shi Lei , Nan Xue , Wang Yunfang , Yue Wen , Pei Xuetao 
Published: December 2010

AbstractEpimorphin/syntaxin 2 is a high conserved and very abundant protein involved in epithelial morphogenesis in various organs. We have shown recently that epimorphin (EPM), a protein exclusively expressed on the surface of hepatic stellate cells and myofibroblasts of the liver, induces bile duct formation of hepatic stem‐like cells (WB‐F344 cells) in a putative biophysical way. Therefore, the aim of this study was to present some of the molecular mechanisms by which EPM mediates bile duct formation. We established a biliary differentiation model by co‐culture of EPM‐overexpressed mesenchymal cells (PT67EPM) with WB‐F344 cells. Here, we showed that EPM could promote WB‐F344 cells differentiation into bile duct‐like structures. Biliary differentiation markers were also elevated by EPM including Yp, Cx43, aquaporin‐1, CK19, and gamma glutamyl transpeptidase (GGT). Moreover, the signaling pathway of EPM was analyzed by focal adhesion kinase (FAK), extracellular regulated kinase 1/2 (ERK1/2), and RhoA Western blot. Also, a dominant negative (DN) RhoA‐WB‐F344 cell line (WBRhoA‐DN) was constructed. We found that the levels of phosphorylation (p) of FAK and ERK1/2 were up‐regulated by EPM. Most importantly, we also showed that RhoA is necessary for EPM‐induced activation of FAK and ERK1/2 and bile duct formation. In addition, a dual luciferase‐reporter assay and CHIP assay was performed to reveal that EPM regulates GGT IV and GGT V expression differentially, possibly mediated by C/EBPβ. Taken together, these data demonstrated that EPM regulates bile duct formation of WB‐F344 cells through effects on RhoA and C/EBPβ, implicating a dual aspect of this morphoregulator in bile duct epithelial morphogenesis. J. Cell. Physiol. 226: 2807–2816, 2011. © 2011 Wiley‐Liss, Inc.
Inhibitory action of neem aqueous extract (azadirachta indica A. Juss) on the vitellogenesis of rhipicephalus sanguineus (Latreille, 1806) (Acari: Ixodidae) ticks
Authors: Eloisi Denardi Sandra , Henrique Bechara Gervásio , Rosa de Oliveira Patrícia , Camargo Mathias Maria Izabel 
Published: December 2010

AbstractThe present study revealed unheard of data about the action of aqueous extracts of neem leaves (Azadirachta indica) on the vitellogenesis of Rhipicephalus sanguineus ticks, proving that these extracts in 10 and 20% concentrations do not have the potential to kill the females; however, in lower concentrations (10%) provokes great morphological alterations in germinative cells such as the emergence of extended cytoplasmic vacuolization areas as well as the fragmentation of the germinal vesicle, even in those oocytes which were in initial stages of development (I–III), showing that neem is a potent agent which acts impeding one of the main metabolic stages of the ticks, i.e., the reproduction. In oocytes in final stages of development (IV–V) azadirachtin (neem's active principle) caused significant reduction in the size and quantity of proteic granules of the yolk and the inversion of their localization where the smaller granules before inside the cell (normal oocyte) were posteriorly observed in the periphery, and the bigger ones in the central region. Thus, the study showed that the alterations found both in the oocytes and in the pedicel cells indicated that azadirachtin acts on the process of tick's reproduction and signalizes that this plant can be used in the future to control ticks with the advantage of not being aggressive to nontarget organism or the environment. Furthermore, data here obtained showed that the most significant efficiency of the aqueous extract of neem is related to the concentration of 10%, proving that higher doses would not be so efficient. Microsc. Res. Tech. 2010. © 2010 Wiley‐Liss, Inc.
Abundance of antennal chemosensilla in two parasitoid wasps with different degree of host specificity may explain sexual and species differences in their response to host‐related volatiles
Authors: Das Prithwiraj , Chen Li , Sharma Kavita R. , Fadamiro Henry Y. 
Published: December 2010

AbstractThe endoparasitic wasps (Hymenoptera: Braconidae), Microplitis croceipes (specialist), and Cotesia marginiventris (generalist) are parasitoids of lepidopteran larvae that differ in their degree of host specificity. Previous studies by our group have reported key differences in the response of both parasitoids to host‐related volatiles. To possibly understand the basis for our results, we compared the abundance of antennal sensilla in both sexes of both parasitoid species using scanning electron microscopy and silver‐staining techniques. Males of both species had relatively longer antennae than conspecific females. Five major sensilla types were recorded in both species: sensilla chaetica (nonporous), s. trichodea (nonporous), s. placodea (multiporous), s. basiconica (two types, type 1 with terminal opening and type 2 with wall pores), and s. coeloconica (nonporous). Silver staining confirmed multiple pores on the walls of s. placodea and s. basiconica type 2 and terminal openings on s. basiconica type 1, suggesting chemosensory function. In general, both putative chemosensilla types, s. placodea and s. basiconica, were more abundant in M. croceipes (specialist) than in C. marginiventris (generalist), and this was true for both sexes. Comparing the sexes, s. placodea and s. trichodea were significantly more abundant in M. croceipes males compared to females. In contrast, s. placodea was relatively more abundant in female C. marginiventris than in males. These results may explain the reported differences in the responses of both parasitoid species to host‐related volatiles and are discussed in relation to the possible roles of the sensilla types in the behavioral ecology of the parasitoids. Microsc. Res. Tech. 2010. © 2010 Wiley‐Liss, Inc.
Evolving architectural patterns in microbial colonies development
Authors: Gómez‐Aguado F. , Alou L. , Corcuera M.T. , Sevillano D. , Alonso M.J. , Gómez‐Lus M.L. , Prieto J. 
Published: December 2010

AbstractSemithin sections of colonies of three ATCC strains (Staphylococcus aureus, Escherichia coli, and Candida albicans) showed that their internal structure had specific patterns that evolved over the time. These patterns generally were defined by the presence of different layers composed of microorganisms with variable population densities and dead cells. The observed structures in this study could be explained as a particular form of biofilm with an air‐semisolid interface. Microsc. Res. Tech. 2010. © 2010 Wiley‐Liss, Inc.
Synthesis of vinylindoles and vinylpyrroles by the Peterson olefination or by use of the Nysted reagent
Authors: Noland Wayland E. , Etienne Christopher L. , Lanzatella Nicholas P. 
Published: December 2010

AbstractVinylindoles and vinylpyrroles were prepared from their corresponding aldehydes or ketones using the Peterson olefination, or by use of the Nysted reagent, a commercially available gem‐dimetallic compound. The two methods provide efficient and convenient access to these useful heterocyclic 1,3‐diene systems. J. Heterocyclic Chem., (2011).
The synthesis of radiolabeled Irbesartan using N,N‐dimethyl[14C]formamide as a source of carbon‐14 isotope
Authors: Ekhato I. Victor , Bonacorsi Samuel 
Published: December 2010

AbstractIrbesartan (Avapro™) is in clinical use as an antihypertensive drug. It is a nonpeptide angiotensin II receptor antagonist. A radiolabeled version of this drug, intended for use in environmental fate studies, was prepared from N,N‐dimethyl[14C]formamide. Our method of synthesis gave 18% overall yield and a radiochemical purity of 98.22%. Copyright © 2010 John Wiley & Sons, Ltd.
Two Concepts Changing Laser Scribing in Photovoltaics
Authors: Endert Heinrich , Niederhofer Jürgen 
Published: December 2010

AbstractEven in established Photovoltaics (PV) production processes, such as laser structuring of thin‐film cells, there is always a need for further improvement. In a new scribing concept, several compact lasers replace high‐power lasers. This approach simplifies the system setup, reduces costs and is more flexible. additionally, moving compact laser heads on a gantry along the PV panel provides further opportunities for a fast, stable and scalable scribing process.
Complementarity framework for non‐linear dynamic analysis of skeletal structures with softening plastic hinges
Authors: Sivaselvan M. V. 
Published: December 2010

AbstractIn this paper, we describe an algorithm for the incremental state update of elasto‐plastic systems with softening. The algorithm uses a complementary pivoting technique and is based on casting the incremental state update as a complementarity problem. In developing the algorithm, we take advantage of the special features of solid and structural mechanics problems to achieve good computational performance, and hence the ability to compute numerical solutions to practical size problems. For example, the notion of a tangent stiffness matrix arises. Numerical examples using models of skeletal structures are presented to demonstrate the practicability of the algorithm. The numerical examples also raise some interesting questions about multiplicity of the solutions. Copyright © 2010 John Wiley & Sons, Ltd.
Dynamic fracture modeling in shell structures based on XFEM
Authors: Larsson Ragnar , Mediavilla Jesus , Fagerström Martin 
Published: December 2010

AbstractThrough‐the‐thickness crack propagation in thin‐walled structures is dealt with in this paper. The formulation is based on the cohesive zone concept applied to a kinematically consistent shell model enhanced with an XFEM‐based discontinuous kinematical representation. The resulting formulation comprises the representation of continuous deformation, represented by midsurface placement, director and thickness inhomogeneous fields, and discontinuous deformation, represented by discontinuous placement and director fields. The shell model is implemented both for the implicit static analysis and in the context of explicit dynamic integration pertinent to impact loading, and the implementation results in a 7‐parameter solid‐shell element based on a 6‐noded triangular element. In order to properly formulate the dynamic fracture characteristics, a rate‐dependent cohesive zone model is employed with respect to, e.g. limiting crack speeds as observed experimentally. In the final example, this model has been applied to a blast loaded pressure vessel that has been experimentally tested. The results indicate that the right crack speed as well as fracture characteristics are relatively well captured. Furthermore, it appears that the discontinuous model exhibits the expected properties with respect to critical time step size in the dynamic analysis and convergence behavior towards the analytical static solution. Copyright © 2010 John Wiley & Sons, Ltd.
Direct numerical simulation of saturated deformable porous media using parallel hybrid Lattice–Boltzmann and finite element method
Authors: Khan Irfan , Aidun Cyrus K. 
Published: December 2010

AbstractNumerical techniques for modeling saturated deformable porous media have mainly been based on mixture theory or homogenization techniques. However, these techniques rely on phenomenological relationships for the constitutive equations along with assumptions of homogeneous and isotopic material properties to obtain closure. Direct numerical simulations of the multiphasic problem for flow in deformable porous media avoid such assumptions and thus can provide significantly accurate understanding of the physics involved. They serve as a tool to investigate the constitutive relationships in complex geometries. They also allow the validation of the existing mixture theory models and determine their limitations.In this work, a parallel hybrid method using Lattice Boltzmann Method (LBM) for fluid phase and Finite Element Method (FEM) for solid phase is used for direct numerical simulation of saturated deformable porous media. The method provides a number of unique features including scalability on distributed computing necessary for such a problem. The method has been validated for modeling fluid–structure interactions in complex geometries against a number of experimental and analytical solutions. Further some challenging problems has been chosen to show the capability of the method. Copyright © 2010 John Wiley & Sons, Ltd.
In vitro growth and activity of chondrocytes on three dimensional polycaprolactone/chitosan scaffolds
Authors: Jiang Xiaobing , Lu Xiaoling , Xiao Bo , Wan Ying , Zhao Yongxiang 
Published: December 2010

AbstractPorous polycaprolactone/chitosan blend scaffolds with various compositional proportions were prepared using a particulate‐leaching method. The pore parameters of resultant scaffolds were found to be mainly modulated by porogen. The compressive mechanical properties and hydrophilicity of scaffolds were examined by measuring their compressive modulus and stress strength as well as swelling index. Selected chondrocytes isolated from articular cartilage of knee joints of rabbits were seeded on these scaffolds, and further in vitro cultured for various periods. The growth and activity of seeded cells were estimated by counting numbers of cells proliferated on scaffolds and measuring the amounts of proteoglycans and type II collagen synthesized by the seeded cells. It was found that some scaffolds composed of proper component ratios and having appropriate pore parameters exhibited promising characteristics for the adhesion and proliferation of seeded cells while maintaining the phenotype and activity of the cells. Copyright © 2010 John Wiley & Sons, Ltd.
BMS‐790052 – ein oral verfügbares Hepatitis‐C‐Virustatikum in der Pipeline
Authors: Schmitz Jens 
Published: December 2010

AbstractIn Deutschland leiden gegenwärtig ca. 500.000 Menschen an einer chronischen Hepatitis‐C‐Virus (HCV)‐Infektion. Die weltweite Prävalenz wird auf 170 Millionen Menschen geschätzt. Derzeit gilt die Behandlung mit pegyliertem Interferon‐α (PEG‐INF‐α) in Kombination mit Ribavirin über mehrere Monate als Standardtherapie. Die Ansprechrate ist jedoch bei Infektionen mit dem weitverbreiteten Virusgenotyp HCV‐1 nur ca. 50 %. Die Entwicklung von Wirkstoffen, die gegen alle HCV‐Genotypen aktiv sind, ist also dringend notwendig.
Impfstoffe gegen die Virushepatitiden A bis E. Was haben sie bewirkt, was können sie bewirken?
Authors: Jilg Wolfgang 
Published: December 2010

AbstractWir verfügen gegenwärtig über wirksame und sehr gut verträgliche Impfstoffe gegen Hepatitis A und B. Der Hepatitis‐A‐Impfstoff enthält formalininaktiviertes, adjuvantiertes Hepatitis‐A‐Virus. Die Schutzrate nach zwei Impfungen ist nahezu 100 %, die Schutzdauer beträgt mindestens 20 Jahre. Die Impfung ist in erster Linie indiziert für Reisende in Hepatitis‐A‐Endemiegebiete Afrikas, Asiens und Südamerikas. Der Impfstoff gegen Hepatitis B besteht aus HBsAg, dem Oberflächenprotein des Hepatitis‐B‐Virus, das heute gentechnisch hergestellt wird. Auch dieser Impfstoff ist adjuvantiert. Die Grundimmunisierung mit drei Impfdosen führt in ca. 95 % der Geimpften zum Erfolg und verleiht für 10 bis 15 Jahre einen Schutz vor Infektion oder zumindest vor Erkrankung. Ob für einen lebenslangen Schutz Nachimpfungen notwendig sind, ist noch unklar. Die Impfung wird seit 1995 in Deutschland für alle Kinder im ersten Lebensjahr empfohlen. Geimpft werden sollten auch alle mit erhöhter Hepatitis‐B‐Gefährdung, z.B. medizinisches Personal. Der Hepatitis‐B‐Impfstoff schützt auch gegen das nur mit dem Hepatitis‐B‐Virus zusammen auftretende Hepatitis‐D‐Virus.Nach wie vor gibt es keinen Impfstoff gegen Hepatitis C, dagegen wurde ein experimenteller Impfstoff gegen Hepatitis E bereits erfolgreich eingesetzt.
Leitliniengerechte Therapie der Hepatitis C. Die Eradikation von HCV als Ziel
Authors: Sarrazin Christoph , Zeuzem Stefan 
Published: December 2010

AbstractFür die Hepatitis C gilt weiterhin, dass bei dem Großteil der Patienten die Infektion bisher nicht bekannt ist. Die chronische Hepatitis C stellt jedoch eine der Hauptursachen für die Leberzirrhose und das Leberzellkarzinom in Deutschland dar. Ein breites Screening mithilfe des HCV‐Antikörperassays bei Patienten mit unklar erhöhten Leberwerten bzw. unklarer Lebererkrankung aber auch bei allen Personen mit entsprechenden Risikofaktoren für die Übertragung des Virus ist daher von besonderer Wichtigkeit. Die Indikation zur antiviralen Therapie der chronischen HCV‐Infektion ist großzügig zu stellen. Die Behandlung besteht aus der kombinierten Gabe von pegyliertem Interferon alfa und Ribavirin. Auf der Grundlage von zahlreichen Studien sind Stoppregeln zur frühzeitigen Beendigung der Behandlung, sowie Regeln zur Festlegung der optimalen Therapiedauer mithilfe der Ausgangsviruslast und der HCV‐RNA‐Kinetik unter Therapie etabliert worden. Größte Bedeutung kommt dem Management von Nebenwirkungen unter der Therapie zu, um möglichst eine vollständige Therapiedosis und ‐dauer zu erreichen. Durch die aktuell in der klinischen Phase II bis III befindlichen direkt antiviralen Substanzen werden sich die Heilungsraten der Therapie in naher Zukunft wesentlich verbessern.
Continuous and high‐dose cytarabine combined chemotherapy in children with down syndrome and acute myeloid leukemia: Report from the Japanese children's cancer and leukemia study group (JCCLSG) AML 9805 down study
Authors: Taga Takashi , Shimomura Yasuto , Horikoshi Yasuo , Ogawa Atsushi , Itoh Masaki , Okada Masahiko , Ueyama Junichi , Higa Takeshi , Watanabe Arata , Kanegane Hirokazu , Iwai Asayuki , Saiwakawa Yutaka , Kogawa Kazuhiro , Yamanaka Junko , Tsurusawa Masahito 
Published: December 2010

AbstractBackgroundThe aim of the JCCLSG AML 9805 Down study was to evaluate the effect of continuous and high‐dose cytarabine combined chemotherapy on the survival outcome of acute myeloid leukemia (AML) with Down syndrome (DS).ProcedureFrom May 1998 to December 2006, DS patients with newly diagnosed AML were enrolled. Remission induction therapy consisted of two courses of pirarubicin, vincristine, and continuous‐dose cytarabine (AVC1). The patients who achieved complete remission (CR) after two courses of AVC1 were subsequently treated with mitoxantrone and continuous‐dose cytarabine (MC), etoposide and high‐dose cytarabine (EC) and pirarubicin, vincristine, and continuous‐dose cytarabine (AVC2).ResultsTwenty‐four patients were enrolled. All patients were younger than 4 years and diagnosed as having acute megakaryoblastic leukemia. Twenty‐one patients achieved CR. Three patients died during remission induction therapy due to serious infection. No toxic deaths were observed during remission. All but one patient maintained CR without serious complications. The 5‐year overall and event‐free survivals were 87.5% ± 6.8% and 83.1% ± 7.7%, respectively.ConclusionsContinuous and high‐dose cytarabine combined chemotherapy with reduced intensity would be effective in DS children with AML. Pediatr Blood Cancer 2011;57:36–40. © 2011 Wiley‐Liss, Inc.
Determining the test characteristics of claims‐based diagnostic codes for the diagnosis of venous thromboembolism in a medical service claims database
Authors: Tagalakis Vicky , Kahn Susan R 
Published: December 2010

AbstractPurposeTo determine the test characteristics of diagnostic codes within a medical service claims database for deep vein thrombosis (DVT) and pulmonary embolism (PE).MethodsThe Regie de l' Assurance Maladie du Québec (RAMQ) administers the health insurance program in Québec, Canada. RAMQ claims data were obtained for subjects with objectively diagnosed DVT with or without PE who were participants in the Venous Thrombosis Outcomes (VETO) Study from April 2001 to July 2002. Using the date of DVT and PE diagnosis in the VETO record as the reference standard, the proportion of subjects correctly classified by RAMQ diagnostic codes was determined for the exact date of DVT and PE occurrence and for four expanded time windows around this date.ResultsIn all, 355 VETO patients were included, 301 with DVT alone and 54 with DVT and PE. Overall, 97% of VETO cases had a RAMQ diagnostic code for DVT and 82% of VETO cases with PE had a RAMQ diagnostic code for PE. Sensitivity for DVT and PE was 52% (95% confidence interval (CI), 47–57) and 35% (95% CI, 23–49), respectively for the exact date of diagnosis, and 87% (95% CI, 83–90) and 78% (95% CI, 64–88), respectively for a 60‐day window around this date. As all VETO participants had DVT, specificity for the diagnosis of DVT could not be determined.ConclusionDiagnostic codes within a medical service claims database are relatively sensitive indicators for DVT and PE, and use of claims data for VTE research purposes can be considered. Copyright © 2010 John Wiley & Sons, Ltd.