bluuebunny/crossref_metadata_2025_split huggingface dataset:
Allogeneic hematopoietic stem cell transplantation in ovarian cancer—the EBMT experience
Authors: Bay Jacques‐Olivier , Cabrespine‐Faugeras Aurélie , Tabrizi Reza , Blaise Didier , Viens Patrice , Ehninger Gerhard , Bornhauser Martin , Slavin Shimon , Rosti Giovanni , Peccatori Jacopo , Demirer Taner , Bregni Marco 
Published: January 2010

AbstractAlthough preliminary results suggest that allogeneic hematopoietic stem cell transplantation (allo HCT) for ovarian cancer (OC) is a feasible procedure, the low patient number in previous studies had limited ability to evaluate the true benefit of allo HCT in OC. This retrospective multicenter study included 30 patients with OC allografted between 1995 and 2005 to determine the outcome of patients with OC treated with allo HCT. Prior to allo HCT, patients were in complete response (n = 1), partial response (n = 7), stable disease (n = 11) or had progressive disease (n = 13). An objective response (OR) was observed in 50% (95% CI, 33–67) of patients. Three patients of responding patients had an objective response following the development of acute graft‐versus‐host disease (aGvHD). The cumulative incidence of chronic GvHD (cGVHD) was 34% (95% CI, 18–50). Transplant relative mortality rates were 7 and 20% on day 100 and 1 year, respectively. With a median follow‐up of 74.5 months (range 16–148), median progression free survival (PFS) was 6 months and median overall survival (OS) was 10.4 months. Patients who developed cGvHD following allo HCT had a significant OS improvement compared to those who did not (17.6 months vs. 6.5 months, p = 0.042). However, PFS was not similarly significantly improved in patients who developed cGvHD (12 months versus 3.7 months, p = 0.81). Allo HCT in OC may lead to graft‐versus‐OC effects. Their clinical relevance remains to be shown.
Harm reduction therapy: a practice‐friendly review of research
Authors: Logan Diane E. , Marlatt G. Alan 
Published: January 2010

AbstractHarm reduction is an umbrella term for interventions aiming to reduce the problematic effects of behaviors. Although harm reduction was originally and most frequently associated with substance use, it is increasingly being applied to a multitude of other behavioral disorders. This article reviews the state of empirical research on harm reduction practices including alcohol interventions for youth, college students, and a variety of other adult interventions. We also review nicotine replacement and opioid substitution, as well as needle exchanges and safe injection sites for intravenous drug users. Dozens of peer‐reviewed controlled trial publications provide support for the effectiveness of harm reduction for a multitude of clients and disorders without indications of iatrogenic effects. Harm reduction interventions provide additional tools for clinicians working with clients who, for whatever reason, may not be ready, willing, or able to pursue full abstinence as a goal. © 2010 Wiley Periodicals, Inc. J Clin Psychol: In Session 66: 1–14, 2010.
Ion transport in a human lens epithelial cell line exposed to hyposmotic and apoptotic stress
Authors: Chimote Ameet A. , Adragna Norma C. , Lauf Peter K. 
Published: January 2010

AbstractMembrane transport changes in human lens epithelial (HLE‐B3) cells under hyposmotic and apoptotic stress were compared. Cell potassium content, Ki, uptake of the K congener rubidium, Rbi, and water content were measured after hyposmotic stress induced by hypotonicity, and apoptotic stress by the protein‐kinase inhibitor staurosporine (STP). Cell water increased in hyposmotic (150 mOsm) as compared to isosmotic (300 mOsm) balanced salt solution (BSS) by >2‐fold at 5 min and decreased within 15 min to baseline values accompanied by a 40% Ki loss commensurate with cell swelling and subsequent cell shrinkage likely due to regulatory volume decrease (RVD). Loss of Ki, and accompanying water, and Rbi uptake in hyposmotic BSS were prevented by clotrimazole (CTZ) suggesting water shifts associated with K and Rb flux via intermediate conductance K (IK) channels, also detected at the mRNA and protein level. In contrast, 2 h after 2 µM STP exposure, the cells lost ∼40% water and ∼60% Ki, respectively, consistent with apoptotic volume decrease (AVD). Indeed, water and Ki loss was at least fivefold greater after hyposmotic than after apoptotic stress. High extracellular K and 2 mM 4‐aminopyridine (4‐AP) but not CTZ significantly reduced apoptosis. Annexin labeling phosphatidylserine (PS) at 15 min suggested loss of lipid asymmetry. Quantitative PCR revealed significant IK channel expression during prolonged hyposmotic stress. Results suggest in HLE‐B3 cells, IK channels likely partook in and were down regulated after RVD, whereas pro‐apoptotic STP‐activation of 4‐AP‐sensitive voltage‐gated K channels preceded or accompanied PS externalization before subsequent apoptosis. J. Cell. Physiol. 223: 110–122, 2010. © 2009 Wiley‐Liss, Inc.
Cyclophilin C‐associated protein/Mac‐2 binding protein colocalizes with calnexin and regulates the expression of tissue transglutaminase
Authors: Kong Wuyi , Lin Bryan W. , Li Shaowei , Longaker Michael T. , Lorenz H. Peter 
Published: January 2010

AbstractCyclophilin C‐associated protein (CyCAP) or Mac‐2 binding protein has been identified as a binding protein for cyclophilin C in mice and for Mac‐2 (galectin‐3) in human, suggesting its multiple binding activity to proteins. In the present study, using specific anti‐rat‐CyCAP antibody, we found that CyCAP colocalizes with calnexin at the location near the nuclear envelope, however CyCAP does not have colocalization with calreticulin. In senescent fibroblasts and interferon‐gamma (IFNγ) treated fibroblasts, both calnexin and CyCAP form larger polymers and are released from the endoplasmic reticulum (ER) through the cellular membrane to the extracellular area. Immunoprecipitation studies further confirm that the release of calnexin is through binding to CyCAP. Further, we found that tissue transglutaminase (tTG) protein is decreased, however not at the RNA level, in CyCAP null fibroblasts, which suggests that CyCAP is involved in tTG post‐translational modification. Our data give novel evidence that CyCAP regulates the post‐translational modification of tTG through its colocalization with calnexin in ER. J. Cell. Physiol. 223: 151–157, 2010. © 2009 Wiley‐Liss, Inc.
Regulation of ATPase activity of transglutaminase 2 by MT1‐MMP: Implications for mineralization of MC3T3‐E1 osteoblast cultures
Authors: Nakano Yukiko , Forsprecher Jennifer , Kaartinen Mari T. 
Published: January 2010

AbstractA pro‐mineralization function for transglutaminase 2 (TG2) has been suggested in numerous studies related to bone, cartilage, and vascular calcification. TG2 is an enzyme which can perform protein crosslinking functions, or act as a GTPase/ATPase depending upon different stimuli. We have previously demonstrated that TG2 can act as an ATPase in a Ca2+‐rich environment and that it can regulate phosphate levels in osteoblast cultures. In this study, we investigate the role MT1‐MMP in regulating the ATPase activity of TG2. We report that proteolytic cleavage of TG2 by MT1‐MMP in vitro results in nearly a 3‐fold increase in the ATPase activity of TG2 with a concomitant reduction in its protein‐crosslinking activity. We show that MC3T3‐E1 osteoblasts secreted full‐length TG2 and major smaller fragments of 66 and 56 kDa, the latter having ATP‐binding abilities. MT1‐MMP inhibition by a neutralizing antibody suppressed mineralization of osteoblast cultures to 35% of control, and significantly reduced phosphate levels in conditioned medium (CM). Furthermore, MT1‐MMP inhibition abolished two of TG2 fragments in the cultures, one of which, the 56‐kDa fragment, has ATPase activity. Neutralization of MT1‐MMP at early phases of mineralization significantly reduced mineral deposition, but had no effect in later phases implying MT1‐MMP and TG2 might contribute to the initiation of mineralization. The cleavage of TG2 by MT1‐MMP likely occurs on the cell surface/pericellular matrix where MT1‐MMP and TG2 were co‐localized. Based on these data, we propose that MT1‐MMP modulates the extracellular function TG2 as part of a regulatory mechanism activates the pro‐mineralization function of TG2. J. Cell. Physiol. 223: 260–269, 2010. © 2009 Wiley‐Liss, Inc.
Decomposition of 1,1‐dimethyl‐1‐silacyclobutane on a tungsten filament—evidence of both ring CC and ring SiC bond cleavages
Authors: Tong L. , Shi Y. J. 
Published: January 2010

AbstractThe decomposition of 1,1‐dimethyl‐1‐silacyclobutane (DMSCB) on a heated tungsten filament has been studied using vacuum ultraviolet laser single photon ionization time‐of‐flight mass spectrometry. It is found that the decomposition of DMSCB on the W filament to form ethene and 1,1‐dimethylsilene is a catalytic process. In addition, two other decomposition channels exist to produce methyl radicals via the SiCH3 bond cleavage and to form propene (or cyclopropane)/dimethylsilylene. It has been demonstrated that both the formation of ethene and that of propene are stepwise processes initiated by the cleavage of a ring CC bond and a ring SiC bond, respectively, to form diradical intermediates, followed by the breaking of the remaining central bonds in the diradicals. The formation of ethene via an initial cleavage of a ring CC bond is dominant over that of propene via an initial cleavage of a ring SiC bond. When the collision‐free condition is voided, secondary reactions in the gas‐phase produce various methyl‐substituted 1,3‐disilacyclobutane molecules. The dominant of all is found to be 1,1,3,3‐tetramethyl‐1,3‐disilacyclobutane originated from the dimerization of 1,1‐dimethylsilene. Copyright © 2010 John Wiley & Sons, Ltd.
Femtosecond vs. Picosecond Laser Material Processing
Authors: Tuennermann Andreas , Nolte Stefan , Limpert Jens 
Published: January 2010

AbstractLaser materials processing has been an intensive research topic since the invention of the laser. nowadays, lasers are used as efficient and qualified tools in many industrial processes, like heavy industrial cutting, hardening, and welding. however, as the miniaturization of components and devices is progressing, finer structures are required. here, the flexible laser processing with conventional laser sources is typically limited by thermal or mechanical damage (melting, formation of burr and cracks, changes in the morphology etc.), which is especially true if metals have to be processed. In order to overcome these limitations and to minimize collateral damage various research activities based on the use of ultrashort laser pulses with picosecond or femtosecond duration have been started in the early 1990s (e.g. [1–8]).
Adaptation of the curve number approach to runoff estimation for loamy soils over a growing season for winter wheat: Comparison with the STREAM approach
Authors: Martin P. , Ouvry J. F. , Bockstaller C. 
Published: December 2009

AbstractThis paper proposes an adaptation of the curve number (CN) approach to runoff estimation in an agricultural context. The approach is proposed for the north‐western European loess belt regions whose soils are very sensitive to surface crusting which can lead to runoff, soil erosion and muddy flows. The likelihood of runoff is assessed from the sequence of soil surface states generated by cultivation practices. The new approach is based on the calculation of CN values for each stage of the soil‐surface‐state sequence. The approach is described for winter‐sown wheat, which is the main crop of the European loess belt. The authors define a sequence made up of six stages from wheat sowing to sowing of a green manure (mustard) after wheat harvest. Use of a long‐term network of experimental plots set up in Upper Normandy enables protocols to be defined for calculating runoff for each of these stages. Results were compared to those obtained with the STREAM model. Runoff calculated from the CNs appeared to agree with the measured values, whereas results obtained with the STREAM model are less accurate. Starting from there, the authors provide the basis of a diagnostic tool based on the new CN approach using the soil surface conditions characterisation for adjusting CN data for cultivation practices not yet tested on experimental plots. Copyright © 2009 John Wiley & Sons, Ltd.
Eugenol precludes cutaneous chemical carcinogenesis in mouse by preventing oxidative stress and inflammation and by inducing apoptosis
Authors: Kaur Gurpreet , Athar Mohammad , Alam M. Sarwar 
Published: December 2009

AbstractThe present study was designed to investigate the protective efficacy of eugenol against skin cancer and probe into the mechanistic aspects. Skin tumors were initiated by applying 160 nmol DMBA and promoted by twice weekly applications of 8.5 nmol TPA for 28 wk. All mice developed tumors by 13 wk of promotion. However, in mice pretreated with 30 µL eugenol, no tumors were detected until 8 wk (following anti‐initiation protocol) and until 14 wk (following antipromotion protocol) of tumor promotion. PCNA and TUNEL immunohistochemistry of tumors revealed eugenol to ameliorate cell proliferation and elevate apoptosis respectively. The effect of eugenol was assessed on specific stages of carcinogenesis. Initiation with DMBA led to a significant upregulation of p53 expression with a concomitant increase in p21WAF1 levels in epidermal cells indicating induction of damage to the DNA. However, pretreatment with eugenol led to overexpression of these genes, which probably helped stimulate apoptosis of the initiated cells. To ascertain the molecular mechanisms implicated in the antitumor promoting activity of eugenol, its effect was investigated on markers of tumor promotion and inflammation: ODC activity and iNOS and COX‐2 expression, and on levels of proinflammatory cytokines (IL‐6, TNF‐α, and PGE2). Eugenol markedly inhibited all. Eugenol also inhibited the upstream signaling molecule: NF‐κB, which regulates the expression of these genes. TPA‐induced depletion of cutaneous GSH and antioxidant enzymes armory was also precluded by eugenol. From these results, it could be concluded that eugenol markedly protects against chemically induced skin cancer and acts possibly by virtue of its antiproliferative, anti‐inflammatory, and antioxidant activities. © 2009 Wiley‐Liss, Inc.
Vitamin D receptor ligands, adenomatous polyposis coli, and the vitamin D receptor FokI polymorphism collectively modulate β‐catenin activity in colon cancer cells
Authors: Egan Jan B. , Thompson Patricia A. , Vitanov Milen V. , Bartik Leonid , Jacobs Elizabeth T. , Haussler Mark R. , Gerner Eugene W. , Jurutka Peter W. 
Published: December 2009

AbstractThe activity of β‐catenin, commonly dysregulated in human colon cancers, is inhibited by the vitamin D receptor (VDR), and this mechanism is postulated to explain the putative anti‐cancer activity of vitamin D metabolites in the colon. We investigated the effect of a common FokI restriction site polymorphism (F/f) in the human VDR gene as well as the effect of anti‐tumorigenic 1,25‐dihydroxyvitamin D3 (1,25D) and pro‐tumorigenic lithocholic acid (LCA) VDR ligands on β‐catenin transcriptional activity. Furthermore, the influence of a major regulatory protein of β‐catenin, the APC tumor suppressor gene, on VDR‐dependent inhibition of β‐catenin activity was examined. We report herein that β‐catenin‐mediated transcription is most effectively suppressed by the VDR FokI variant F/M4 when 1,25D is limiting. Using Caco‐2 colorectal cancer (CRC) cells, it was observed that VDR ligands, 1,25D and LCA, both suppress β‐catenin transcriptional activity, though 1,25D exhibited significantly greater inhibition. Moreover, 1,25D, but not LCA, suppressed endogenous expression of the β‐catenin target gene DKK‐4 independent of VDR DNA‐binding activity. These results support β‐catenin sequestration away from endogenous gene targets by 1,25D–VDR. This activity is most efficiently mediated by the FokI gene variant F/M4, a VDR allele previously associated with protection against CRC. Interestingly, we found the inhibition of β‐catenin activity by 1,25D–VDR was significantly enhanced by wild‐type APC. These results reveal a previously unrecognized role for 1,25D–VDR in APC/β‐catenin cross talk. Collectively, these findings strengthen evidence favoring a direct effect on the Wnt‐signaling molecule β‐catenin as one anti‐cancer target of 1,25D–VDR action in the colorectum. © 2009 Wiley‐Liss, Inc.
Total obstetric brachial plexus palsy: Results and strategy of microsurgical reconstruction
Authors: El‐gammal Tarek A. , El‐Sayed Amr , Kotb Mohamed M. , Ragheb Yasser Farouk , Saleh Waleed Riad , Elnakeeb Ramy Mohamed , El‐Sayed Semaya Ahmad 
Published: January 2010

AbstractFrom 2000 to 2006, 35 infants with total obstetric brachial plexus palsy underwent brachial plexus exploration and reconstruction. The mean age at surgery was 10.8 months (range 3–60 months), and the median age was 8 months. All infants were followed for at least 2.5 years (range 2.5–7.3 years) with an average follow‐up of 4.2 years. Assessment was performed using the Toronto Active Movement scale. Surgical procedures included neurolysis, neuroma excision and interposition nerve grafting and neurotization, using spinal accessory nerve, intercostals and contralateral C7 root. Satisfactory recovery was obtained in 37.1% of cases for shoulder abduction; 54.3% for shoulder external rotation; 75.1% for elbow flexion; 77.1% for elbow extension; 61.1% for finger flexion, 31.4% for wrist extension and 45.8% for fingers extension. Using the Raimondi score, 18 cases (53%) achieved a score of three or more (functional hand). The mean Raimondi score significantly improved postoperatively as compared to the preoperative mean: 2.73 versus 1, and showed negative significant correlation with age at surgery. In total, obstetrical brachial plexus palsy, early intervention is recommended. Intercostal neurotization is preferred for restoration of elbow flexion. Tendon transfer may be required to improve external rotation in selected cases. Apparently, intact C8 and T1 roots should be left alone if the patient has partial hand recovery, no Horner syndrome, and was operated early (3‐ or 4‐months old). Apparently, intact nonfunctioning lower roots with no response to electrical stimulation, especially in the presence of Horner syndrome, should be neurotized with the best available intraplexal donor. © 2010 Wiley‐Liss, Inc. Microsurgery, 2010.
Complete free flap loss due to extensive hemodilution
Authors: Namdar Thomas , Bartscher Tanja , Stollwerck Peter L. , Mailänder Peter , Lange Thomas 
Published: January 2010

AbstractBackground: Free tissue transfer in reconstruction of lower extremity wounds is well established. Controversy surrounds type and regimen of intravenous fluid application during microsurgery. Hemodilution is supposed to influence haemostatic process. Patients and Methods: We performed an analysis of 48 patients treated with a free latissimus dorsi muscle flap to the lower leg for posttraumatic soft‐tissue coverage. Postoperative latissimus dorsi muscle flap perfusion was controlled by clinical monitoring. Intraoperative infusion management was evlatuated retrospectively. Results: In 4 of 48 included patients, a complete loss of free latissimus dorsi muscle flap was registered. Concomitant increased saline infusion was detected (4,534 ml versus. 6,125 ml; P = 0.048). Similar findings for relation of total infusion volume to body weight were seen (44 ml/kg versus 69 ml/kg; P = 0.01). No significant colloid infusion was detected. Conclusions: We demonstrate the clinical relevance of extensive intraoperative hyperhydration, which can provoke a complete free flap loss. © 2010 Wiley‐Liss, Inc. Microsurgery, 2010.
Surgical complications in kidney transplantation in nonhuman primates
Authors: Song Lijun , Qi Shijie , Dun Hao , Hu Yanxin , Ma Anlun , Yu Guang , Xiong Zuquan , Zhu Shenyun , Wang Xiang , Xu Dasheng , Li Gang , Shang Yupu , Kinugasa Fumitaka , Sudo Yuji , Bai Jieying , Zeng Lin , Daloze Pierre , Chen Huifang 
Published: January 2010

AbstractSurgical complications are important causes of graft loss in the nonhuman primate kidney transplantation model. We reviewed the incidence and intervention methods in 182 kidney transplantations performed in our lab recently 2 years in Cynomolgus monkeys. There were six renal artery thromboses (3.3%), eight urine leakages (4.4%), and five ureteral stenoses (2.7%). All renal artery thrombosis cases were found within 3 days after surgery. Urine leakage appeared from the 5th to 12th day after surgery and all cases were caused by ureter rupture. Reexploration was performed in five cases to reanastomose ureter with stent. Four cases reached long‐term survival. The rest one died of graft rejection. Ureteral stenoses were found in long‐term survival cases. Ureter reanastomoses with stent were performed in two cases. The postoperative renal functions of these two monkeys recovered to normal and they survived until study termination. From this large number of study, our experience indicated that kidney transplantation in the nonhuman primate is a safe procedure with low complications. Reexploration is recommended for salvage of the graft with urine leakage and ureteral stenosis. © 2010 Wiley‐Liss, Inc. Microsurgery, 2010.
Expression pattern of acetylated α‐tubulin in porcine spermatogonia
Authors: Luo Jinping , Rodriguez‐Sosa Jose Rafael , Tang Lin , Bondareva Alla , Megee Susan , Dobrinski Ina 
Published: December 2009

AbstractMammalian spermatogonial stem cells reside on the basement membrane of the seminiferous tubules. The mechanisms responsible for maintenance of spermatogonia at the basement membrane are unclear. Since acetylated α‐tubulin (Ac‐α‐Tu) is a component of long‐lived, stable microtubules and deacetylation of α‐tubulin enhances cell motility, we hypothesized that acetylation of α‐tubulin might be associated with positioning of spermatogonia at the basement membrane. The expression pattern of Ac‐α‐Tu at different stages of testis development was characterized by immunohistochemistry for Ac‐α‐Tu and spermatogonia‐specific proteins (PGP 9.5, DAZL). In immature pig testes, Ac‐α‐Tu was present exclusively in gonocytes at 1 week of age, and in a subset of spermatogonia at 10 weeks of age. At this age, spermatogonia are migrating toward the tubule periphery and Ac‐α‐Tu appeared polarized toward the basement membrane. In adult pig testes, Ac‐α‐Tu was detected in few single or paired spermatogonia at the basement membrane as well as in spermatids and spermatozoa. Only undifferentiated (DAZL−), proliferating (determined by BrdU incorporation) spermatogonia expressed high levels of Ac‐α‐Tu. Comparison with the expression pattern of β‐tubulin and tyrosinated α‐tubulin confirmed that only Ac‐α‐Tu is specific to germ cells. The unique pattern of Ac‐α‐Tu in undifferentiated germ cells during postnatal development suggests that posttranslational modifications of microtubules may play an important role in recruiting and anchoring spermatogonia at the basement membrane. Mol. Reprod. Dev. 77: 348–352, 2010. © 2009 Wiley‐Liss, Inc.
Purposeful commingling of adult and child cranial elements from the Chelechol ra Orrak Cemetery, Palau
Authors: Fitzpatrick S. M. , Nelson G. C. 
Published: December 2009

AbstractContinued excavation in 2007 of the early (ca. 2000–3000 calBP) cemetery at Chelechol ra Orrak in Palau, Micronesia revealed the remains of at least six individuals, adding to a minimum number of at least 25 other mostly fragmented individuals recovered in previous years. Of particular interest was the discovery of two isolated adult partial crania one of which, when emptied of sandy matrix, was found to contain a complete child's frontal bone. Here we explore the possibility that the placement of the skulls, particularly the commingling of the adult and child cranial elements, was done purposefully and discuss its significance for early Pacific mortuary behaviours. Copyright © 2009 John Wiley & Sons, Ltd.