bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting slow‐proliferating ovarian cancer cells
Authors: Kondoh Eiji , Mori Seiichi , Yamaguchi Ken , Baba Tsukasa , Matsumura Noriomi , Cory Barnett J. , Whitaker Regina S. , Konishi Ikuo , Fujii Shingo , Berchuck Andrew , Murphy Susan K. 
Published: September 2009

AbstractAdvanced ovarian cancer has a high rate of recurrence and mortality despite relative chemosensitivity at the time of initial treatment. Conventional chemotherapeutic agents typically target rapidly dividing cells. Disease relapse may therefore result from the survival and later emergence of latent slow‐proliferating and/or quiescent cancer cells. We sought to identify drugs that target this cell population and to investigate the influence of these cells on outcome of patients in remission from advanced ovarian cancer. Drugs with increased efficacy against slower proliferating cells were identified using correlation‐based screening of 44,657 compounds tested on the NCI‐60 panel of cancer cell lines. Validation of candidates was performed in comparison with Cisplatin or Paclitaxel and by manipulation of proliferation rates by serum deprivation. Cytostatic and cytocidal effects were evaluated using MTT assays and active caspase‐3 immunocytochemistry. Ki‐67 proliferation indices were determined for tumors from 104 patients in remission. UCN‐01 efficacy was correlated with longer doubling time among the NCI‐60 cell lines (R = 0.54, p < 0.0001) and in a panel of 24 ovarian cancer cell lines (R = 0.42, p = 0.04), whereas this was not the case for Cisplatin (R = −0.10, p = 0.65) and Paclitaxel efficacy correlated with shorter doubling time (R = −0.52, p = 0.009). Cytostatic and cytocidal effects of UCN‐01 were increased in serum‐deprived cells. A low proliferation index was associated with presence of persistent disease at second‐look surgery (p = 0.01) and poor survival (disease‐free survival, p = 0.002; overall survival, p = 0.04). These results suggest that targeting quiescent ovarian cancer cells may be a worthwhile therapeutic approach to improving survival of women with ovarian cancer.
A fluorescence lifetime imaging scanning confocal endomicroscope
Authors: Kennedy Gordon T. , Manning Hugh B. , Elson Daniel S. , Neil Mark A. A. , Stamp Gordon W. , Viellerobe Bertrand , Lacombe François , Dunsby Chris , French Paul M. W. 
Published: September 2009

AbstractWe describe a fluorescence lifetime imaging endomicroscope employing a fibre bundle probe and time correlated single photon counting. Preliminary images of stained pollen grains, eGFP‐labelled cells exhibiting Förster resonant energy transfer and tissue autofluorescence are presented. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Interactive effects of mechanical stretching and extracellular matrix proteins on initiating osteogenic differentiation of human mesenchymal stem cells
Authors: Huang Chien‐Hsun , Chen Min‐Huey , Young Tai‐Horng , Jeng Jiiang‐Huei , Chen Yi‐Jane 
Published: September 2009

AbstractHuman mesenchymal stem cells (hMSCs) are characterized by their abilities to differentiate into different lineages, including osteoblasts. Besides soluble factors, mechanical strain and extracellular matrix (ECM) proteins play important roles in osteogenic differentiation of hMSCs. However, interactions between them are still not fully understood. The purpose of this study was to investigate the combined effects of insoluble chemical and mechanical factors (ECM proteins vs. cyclic stretching) in driving hMSCs into osteogenic differentiation. To avoid the influence from osteogenic supplements, hMSCs were cultured in regular medium and subjected to cyclic mechanical stretching using a Flexcell Tension system (3% elongation at 0.1 Hz) when they were grown on substrates coated with various ECM proteins (collagen I (Col I), vitronectin (VN), fibronectin (FN), and laminin (LN)). Using alkaline phosphatase (ALP) activity and mineralized matrix deposition as respective indicators of the early and late stages of osteogenesis, we report herein that all of the ECM proteins tested supported hMSC differentiation into osteogenic phenotypes in the absence of osteogenic supplements. Moreover, cyclic mechanical stretching activated the phosphorylation of focal adhesion kinase (FAK), upregulated the transcription and phosphorylation of core‐binding factor alpha‐1 (Cbfa1), and subsequently increased ALP activity and mineralized matrix deposition. Among the ECM proteins tested, FN and LN exhibited greater effects of supporting stretching‐induced osteogenic differentiation than did Col I and VN. The ability of ECM proteins and mechanical stretching to regulate osteogenesis in hMSCs can be exploited in bone tissue engineering via approximate matrix design or application of mechanical stimulation. J. Cell. Biochem. 108: 1263–1273, 2009. © 2009 Wiley‐Liss, Inc.
Local flip angle correction for improved volume T1‐quantification in three‐dimensional dGEMRIC using the look‐locker technique
Authors: Siversson Carl , Tiderius Carl‐Johan , Dahlberg Leif , Svensson Jonas 
Published: September 2009

AbstractPurposeTo present an evaluation method for three‐dimensional Look‐Locker (3D‐LL) based T1 quantification, calculating correct T1 values independent of local flip angle (FA) variations. The method was evaluated both in phantoms and in vivo in a delayed Gadolinium Enhanced MRI of Cartilage (dGEMRIC) study with 33 subjects.Materials and MethodsT1 was measured with 3D‐LL, using both local FA correction and a precalculated FA slice profile, and compared with standard constant FA correction, for all slices in phantoms and in both femur condyles in vivo. T1 measured using two‐dimensional Inversion Recovery (2D‐IR) was used as gold standard.ResultsDue to the FA being slice dependent, the standard constant FA correction results in erroneous T1 (systematic error = 109.1 ms in vivo), especially in the outer slices. With local FA correction, the calculated T1 is excellent for all slices in phantoms (<5% deviation from 2D‐IR). In vivo the performance is lower (systematic error = −57.5 ms), probably due to imperfect inversion. With precalculated FA correction the performance is very good also in vivo (systematic error = 13.3 ms).ConclusionWith the precalculated FA correction method, the 3D‐LL sequence is robust enough for in vivo dGEMRIC, even outside the centermost slices. J. Magn. Reson. Imaging 2009;30:834–841. © 2009 Wiley‐Liss, Inc.
Differentiating multiple sclerosis from other causes of demyelination using diffusion weighted imaging of the corpus callosum
Authors: Straus Farber Rebecca , DeVilliers Laetitia , Miller Aaron , Lublin Fred , Law Meng , Fatterpekar Girish , Delman Bradley , Naidich Thomas 
Published: September 2009

AbstractPurposeTo compare diffusion weighted imaging metrics in gray and white matter brain regions of patients diagnosed with multiple sclerosis (MS) to those diagnosed with secondary demyelinating diseases such as neurosarcoid and acute disseminated encephalomyelitis (ADEM).Materials and MethodsDiffusion weighted scans were performed and apparent diffusion coefficients of 12 regions of interest were determined in 30 MS patients, 21 neurosarcoid patients, and 4 ADEM patients.ResultsMean apparent diffusion coefficients were significantly higher in MS patients than in non‐MS patients in 6 of 6 of the corpus callosal regions assessed but not in any of the non‐callosal white or gray matter regions assessed.ConclusionElevated apparent diffusion coefficients within the corpus callosum on diffusion weighted imaging may potentially help differentiate between patients with MS and patients with other diseases affecting the central nervous system white matter. J. Magn. Reson. Imaging 2009;30:732–736. © 2009 Wiley‐Liss, Inc.
Mutual effects of caveolin and nerve growth factor signaling in pig oligodendrocytes
Authors: Schmitz Matthias , Klöppner Sabine , Klopfleisch Steve , Möbius Wiebke , Schwartz Peter , Zerr Inga , Althaus Hans H. 
Published: September 2009

AbstractSignaling of growth factors may depend on the recruitment of their receptors to specialized microdomains. Previous reports on PC12 cells indicated an interaction of raft‐organized caveolin and TrkA signaling. Because porcine oligodendrocytes (OLs) respond to nerve growth factor (NGF), we were interested to know whether caveolin also plays a role in oligodendroglial NGF/TrkA signaling. OLs expressed caveolin at the plasma membrane but also intracellularly. This was partially organized in the classically Ω‐shaped invaginations, which may represent caveolae. We could show that caveolin and TrkA colocalize by using a discontinuous sucrose gradient (Song et al. [1996] J. Biol. Chem. 271:9690–9697), MACS technology, and immunoprecipitation. However, differential extraction of caveolin and TrkA with Triton X‐100 at 4°C indicated that caveolin and TrkA are probably not exclusively present in detergent‐resistant, caveolin‐containing rafts (CCRs). NGF treatment of OLs up‐regulated the expression of caveolin‐1 (cav‐1) and stimulated tyrosine‐14 phosphorylation of cav‐1. Furthermore, OLs were transfected with cav‐1‐specific small interfering RNA (siRNA). A knockdown of cav‐1 resulted in a reduced activation of downstream components of the NGF signaling cascade, such as p21Ras and mitogen‐activated protein kinase (MAPK) after NGF exposure of OLs. Subsequently, increased oligodendroglial process formation via NGF was impaired. The present study indicates that CCRs/caveolin could play a modulating role during oligodendroglial differentiation and regeneration. © 2009 Wiley‐Liss, Inc.
Interaction of Rab31 and OCRL‐1 in oligodendrocytes: Its role in transport of mannose 6‐phosphate receptors
Authors: Rodriguez‐Gabin A.G. , Ortiz E. , Demoliner K. , Si Q. , Almazan G. , Larocca J.N. 
Published: September 2009

AbstractRab31, a protein that we cloned from an oligodendrocyte cDNA library, is required for transport of mannose 6‐phosphate receptors (MPRs) from the trans‐Golgi network (TGN) to endosomes and for Golgi/TGN organization. Here we extend the knowledge of the mechanism of action of Rab31 by demonstrating its interaction with OCRL‐1, a phosphatidylinositol 4,5‐diphosphate 5‐phosphatase (PI(4,5)P2 5‐phosphatase) that regulates the levels of PI(4,5)P2 and PI(4)P, molecules involved in transport and Golgi/TGN organization. We show that Rab31 interacts with OCRL‐1 in a yeast two‐hybrid system, GST‐Rab31 pull‐down experiments, and coimmunoprecipitation of OCRL‐1 using oligodendrocyte culture lysates. Rab31 and OCRL‐1 colocalize in the TGN, post‐TGN carriers, and endosomes. Cation‐dependent MPR (CD‐MPR) is sorted to OCRL‐1‐containing carriers, but CD63 and vesicular stomatitis virus G (VSVG) are not. siRNA‐mediated depletion of endogenous Rab31 causes collapse of the TGN apparatus and markedly decreases the levels of OCRL‐1 in the TGN and endosomes. Our observations indicate that the role of Rab31 in the Golgi/TGN structure and transport of MPRs depends on its capability to recruit OCRL‐1 to domains of the TGN where the formation of carriers occurs. The importance of our observations is highlighted by the fact that mutation of OCRL‐1 causes demyelination in humans. © 2009 Wiley‐Liss, Inc.
Trimethyltin intoxication up‐regulates nitric oxide synthase in neurons and purinergic ionotropic receptor 2 in astrocytes in the hippocampus
Authors: Latini Laura , Geloso Maria Concetta , Corvino Valentina , Giannetti Stefano , Florenzano Fulvio , Viscomi Maria Teresa , Michetti Fabrizio , Molinari Marco 
Published: September 2009

AbstractNitric oxide (NO) and purinergic ionotropic receptors (P2X) mediate cellular events in the central nervous system (CNS) under physiological conditions as well as during pathological events, and they have been recently proposed to interact in mediating CNS response to injury (Viscomi et al. [2004] Neuroscience 123:393–404; Florenzano et al. [2008] Pflugers Arch. 452:622–644). Trimethyltin (TMT) is an organotin compound that generates neurotoxic effects, and it has been used in a model of neurodegenerative disease and memory dysfunction. TMT causes neuronal death and reactive gliosis primarily in the hippocampus and other limbic regions. In the present study, we examined the degenerative events and the expression of nitric oxide synthase (NOS) and P2X receptor subtypes (P2X1,2,4,7Rs) that were induced by TMT administration at different time points (3, 7, 14, and 21 days) by conventional and confocal microscopy and Western blotting. Massive glial activation and neuronal death in the CA1 and CA3 regions were observed after TMT treatment. In these areas, astrocytic P2X2R and neuronal NOS were temporarily enhanced in association with the progression of neuronal death. In the hippocampus, the physiological expression of P2X1R, P2X4R, and P2X7R was not modified by TMT. The present data demonstrate that, as in other neurodegenerative models, TMT‐induced hippocampal degeneration is associated with nitrergic and purinergic activations. Nevertheless, at odds with previous data, in this model the two systems are active in segregated cell populations, namely, P2XR in astrocytes and NOS in neurons. Finally, the temporal relations between P2XR and NOS expression and neuronal degeneration suggest interactions between P2XR/NO signaling and cell survival. © 2009 Wiley‐Liss, Inc.
A developmental approach to complex PTSD: Childhood and adult cumulative trauma as predictors of symptom complexity
Authors: Cloitre Marylene , Stolbach Bradley C. , Herman Judith L. , Kolk Bessel van der , Pynoos Robert , Wang Jing , Petkova Eva 
Published: September 2009

AbstractExposure to multiple traumas, particularly in childhood, has been proposed to result in a complex of symptoms that includes posttraumatic stress disorder (PTSD) as well as a constrained, but variable group of symptoms that highlight self‐regulatory disturbances. The relationship between accumulated exposure to different types of traumatic events and total number of different types of symptoms (symptom complexity) was assessed in an adult clinical sample (N = 582) and a child clinical sample (N = 152). Childhood cumulative trauma but not adulthood trauma predicted increasing symptom complexity in adults. Cumulative trauma predicted increasing symptom complexity in the child sample. Results suggest that Complex PTSD symptoms occur in both adult and child samples in a principled, rule‐governed way and that childhood experiences significantly influenced adult symptoms.
Brainstem pathology in spasmodic dysphonia
Authors: Simonyan Kristina , Ludlow Christy L. , Vortmeyer Alexander O. 
Published: September 2009

AbstractSpasmodic dysphonia (SD) is a primary focal dystonia of unknown pathophysiology, characterized by involuntary spasms in the laryngeal muscles during speech production. We examined two rare cases of postmortem brainstem tissue from SD patients compared to four controls. In the SD patients, small clusters of inflammation were found in the reticular formation surrounding solitary tract, spinal trigeminal, and ambigual nuclei, inferior olive, and pyramids. Mild neuronal degeneration and depigmentation were observed in the substantia nigra and locus coeruleus. No abnormal protein accumulations and no demyelination or axonal degeneration were found. These neuropathological findings may provide insights into the pathophysiology of SD. Laryngoscope, 2010
Positive family history of essential tremor influences the motor phenotype of Parkinson's disease
Authors: Hedera Peter , Fang John Y. , Phibbs Fenna , Cooper Michael K. , Charles P. David , Davis Thomas L. 
Published: September 2009

AbstractPrevious reports have suggested that essential tremor (ET) represents a risk factor for the development of Parkinson's disease (PD). Patients with long‐standing ET who develop PD tend to have a tremor‐dominant subtype. To further clarify this association, we examined patients from kindreds with autosomal dominant ET who had signs of isolated PD but did not meet criteria for overlapping ET. We identified 22 patients with PD meeting these diagnostic criteria, and 90% (20 of 22) had tremor‐predominant subtype of PD. Unilateral rest tremor was the presenting symptom in 15 of 22 patients, bradykinesia or rigidity in 5 of 22, and gait problems in 2 of 22. Postural tremor was relatively mild, and the severity of kinetic tremor tightly correlated with rest tremor (r = 0.83, P < 0.001). Tremor‐dominant subtype of PD in patients with a positive family history of ET suggests that these patients have inherited a genetic susceptibility factor for tremor, which affects the motor phenotype of PD. © 2009 Movement Disorder Society
Controversies in the Management of Alcoholic Liver Disease
Authors: Tan Hui‐Hui , Virmani Sharad , Martin Paul 
Published: September 2009

AbstractAlcohol is a risk factor for chronic disease burden in developed countries. Alcoholic liver disease affects 1% of the North American population and is the second most frequent indication for liver transplantation in the United States. It is a spectrum that ranges from simple hepatic steatosis to alcoholic hepatitis to steatohepatitis and eventually cirrhosis. The clinical spectrum of alcoholic hepatitis is wide and ranges from the asymptomatic patient to overt liver failure and death. Liver biopsy as a means of prognostication in alcoholic hepatitis has mostly been replaced with less invasive scoring systems. The management of alcoholic liver disease is challenging. Abstinence is the cornerstone of therapy and should include rehabilitation with a multidisciplinary approach. No specific treatment is required in mild to moderate alcoholic hepatitis. In patients with severe hepatitis, there appears to be a moderate survival benefit from the use of either corticosteroids or pentoxifylline in the absence of contraindications to their use. Nonresponders should have steroid therapy withdrawn by day 7, as persistence with therapy is not beneficial. Orthotopic liver transplantation remains the definitive therapy for decompensated alcoholic cirrhosis despite alcohol abstinence. More studies are needed to define the optimal timing of orthotopic liver transplantation and patients at risk of alcohol relapse post‐transplant. Mt Sinai J Med 76:484–498, 2009. © 2009 Mount Sinai School of Medicine
The Bushmeat Trade: Increased Opportunities for Transmission of Zoonotic Disease
Authors: Karesh William B. , Noble Eric 
Published: September 2009

AbstractBushmeat is a term that refers to the use of wild animals, ranging from cane rats to gorillas, for food. The term typically refers to the practice in forests of Africa. The bushmeat trade is an example of an anthropogenic factor that provides opportunities for the transmission of diseases from wildlife to humans. The expansion of the bushmeat trade over the past 2 decades has provided a venue for the emergence of zoonotic diseases by providing an increased opportunity for the transmission of organisms known to cause disease and organisms with an unknown impact on humans. Because the bushmeat trade is embedded in a complex cultural, political, and economic context, efforts to prevent the emergence of zoonoses require a multidisciplinary approach. Mt Sinai J Med 76:429–434, 2009. © 2009 Mount Sinai School of Medicine
Non‐invasive and quantitative evaluation of peripheral vascular resistances in rats by combined NMR measurements of perfusion and blood pressure using ASL and dynamic angiography
Authors: Ménard Jacques C. , Giacomini Eric , Baligand Céline , Fromes Yves , Carlier Pierre G. 
Published: September 2009

AbstractThe in vivo determination of peripheral vascular resistances (VR) is crucial for the assessment of arteriolar function. It requires simultaneous determination of organ perfusion (F) and arterial blood pressure (BP). A fully non‐invasive method was developed to measure systolic and diastolic BP in the caudal artery of rats based on dynamic NMR angiography. A good agreement was found between the NMR approach and the gold standard techniques (linear regression slope = 0.98, R2 = 0.96). This method and the ASL‐MRI measurement of skeletal muscle perfusion were combined into one single NMR experiment to quantitatively evaluate the local vascular resistances in the calf muscle of anaesthetized rats, in vivo and non‐invasively 1) at rest: VR = 7.0 ± 1.0 mmHg·min 100 g·ml−1, F = 13 ±  3 ml min−1.100 g−1 and mean BP (MBP) = 88 ± 10 mmHg; 2) under vasodilator challenge (milrinone): VR = 3.7 ± 1.1 mmHg min.100 g ml−1, F = 21 ± 4 ml min−1.100 g−1 and MBP = 75 ± 14 mmHg; 3) under vasopressor challenge (norepinephrine): VR = 9.8 ± 1.2 mmHg min 100 g ml−1, F = 14 ± 3 ml min−1.100 g−1 and MBP = 137 ± 2 mmHg. Copyright © 2009 John Wiley & Sons, Ltd.
The social processes of interorganizational collaboration and conflict in nonprofit organizations
Authors: Tsasis Peter 
Published: September 2009

AbstractThis article takes a qualitative approach to research in advancing knowledge of the social process by which interorganizational relationships are established and nurtured in nonprofit organizations. The findings reveal that a balance of dependence and autonomy is needed for initiating interorganizational relationships. These relationships are stabilized at the interpersonal level through positive attributes (attitudes, perceptions, and trust) and interpersonal ties of individuals representing their organizations. Sources of conflict, such as value differences, divergent goals, and personality clashes, also influence the working relationships of these organizations.
The ritual dugong bone mounds of Koey Ngurtai, Torres Strait, Australia: Investigating their construction
Authors: Skelly Robert , David Bruno , McNiven Ian J. , Barker Bryce 
Published: September 2009

AbstractThe islands of Western Torres Strait, between Papua New Guinea and Australia, saw the emergence of ritual dugong bone mounds approximately 400 years ago. These mounds were used as a means to commune with, and as an aid for the hunting of, dugongs. This paper explores the bone contents of three dugong bone mounds on the small, uninhabited island of Koey Ngurtai as a means to determine their construction and in doing so to explore the historical emergence of ritual bone mounds associated with dugong hunting magic—and thereby to historicise ethnographically known cultural practices—in Torres Strait. Copyright © 2009 John Wiley & Sons, Ltd.
Made in China at a crossroads: a recourse‐based view
Authors: Zhang Shujun , Su Xiaohua 
Published: September 2009

Abstract
The brand ‘Made in China’ has long stood for low cost and good value. But recent revelations about the quality of some Chinese products exported overseas have begun to put this brand at risk. In the end, much of what constitutes this country brand depends on the strategies Chinese firms pursue, which in turn subject to the firm resources. Drawing from the resource‐base theory, this paper investigates the sources of the competitive advantage of the brand ‘Made in China’ and discusses the choices to be made by the Chinese firms and government in order to maintain a stable and better country brand.
Copyright © 2009 John Wiley & Sons, Ltd.