bluuebunny/crossref_metadata_2025_split huggingface dataset:
Forêtite, a new secondary arsenate mineral from the Cap Garonne mine, France
Authors: Mills S. J. , Kampf A. R. , McDonald A. M. , Favreau G. , Chiappero P.-J. 
Published: July 2012

AbstractForêtite, ideally Cu2Al2(AsO4)(OH,O,H2O)6, is a new secondary arsenate mineral from the Cap Garonne mine, Var, France (IMA2011-100). It has also been identified at the Salsigne gold mine, Languedoc-Roussillon, France. Although it was identified as a potentially new mineral in 1993, a formal description has only been possible as a result of a new find in a chamber called Annex S located near the entrance of the Cap Garonne mine. Forêtite occurs as pale sky blue to aqua aggregates, up to ∼0.1 mm across, made up of minute plates no more than 20 μm in length. It has a very pale blue streak. Individual crystals have a vitreous lustre and are transparent, whereas clusters appear translucent. The calculated density is 3.286 g cm–3. The crystals are brittle, with an irregular fracture and have a hardness of ∼3–4 on Moh's scale. Forêtite is found in direct association with bariopharmacoalumite, cyanotrichite, parnauite, chalcophyllite and mansfieldite in an Al-rich assemblage which is presumed to have formed under acidic conditions. It is biaxial; the average refractive index measured in white light on aggregates of forêtite crystals is 1.620(5). The empirical formula (based on 10 oxygen atoms per formula unit) is Cu1.94(Al1.96Fe0.04)Σ2.00(As0.84S0.09Si0.04)Σ0.97O10H5.19. Raman spectroscopy confirms the presence of OH and H2O in the structure. Forêtite is triclinic, space group P, with a = 6.969(9), b = 7.676(9), c = 8.591(11) Å, α = 82.01(9), β = 71.68(8), γ = 102.68(8)°, V = 415(1) Å3 and Z = 2. The five strongest lines in the X-ray powder diffraction pattern [d in A ˚ , (I), (hkl)] are as follows: 7.307, (100), (010, 00); 3.141, (24), (200, 00); 2.818, (24), (20, 20); 4.519, (23), (111); 2.343, (22), (11). The mineral is named in honour of Dr Jean-Paul Forêt, who co-founded the project that turned the Cap Garonne mine into a protected site and museum.
Stem and progenitor cells in myelodysplastic syndromes show aberrant stage-specific expansion and harbor genetic and epigenetic alterations
Authors: Will Britta , Zhou Li , Vogler Thomas O. , Ben-Neriah Susanna , Schinke Carolina , Tamari Roni , Yu Yiting , Bhagat Tushar D. , Bhattacharyya Sanchari , Barreyro Laura , Heuck Christoph , Mo Yonkai , Parekh Samir , McMahon Christine , Pellagatti Andrea , Boultwood Jacqueline , Montagna Cristina , Silverman Lewis , Maciejewski Jaroslaw , Greally John M. , Ye B. Hilda , List Alan F. , Steidl Christian , Steidl Ulrich , Verma Amit 
Published: July 2012

Abstract
Even though hematopoietic stem cell (HSC) dysfunction is presumed in myelodysplastic syndrome (MDS), the exact nature of quantitative and qualitative alterations is unknown. We conducted a study of phenotypic and molecular alterations in highly fractionated stem and progenitor populations in a variety of MDS subtypes. We observed an expansion of the phenotypically primitive long-term HSCs (lineage−/CD34+/CD38−/CD90+) in MDS, which was most pronounced in higher-risk cases. These MDS HSCs demonstrated dysplastic clonogenic activity. Examination of progenitors revealed that lower-risk MDS is characterized by expansion of phenotypic common myeloid progenitors, whereas higher-risk cases revealed expansion of granulocyte-monocyte progenitors. Genome-wide analysis of sorted MDS HSCs revealed widespread methylomic and transcriptomic alterations. STAT3 was an aberrantly hypomethylated and overexpressed target that was validated in an independent cohort and found to be functionally relevant in MDS HSCs. FISH analysis demonstrated that a very high percentage of MDS HSC (92% ± 4%) carry cytogenetic abnormalities. Longitudinal analysis in a patient treated with 5-azacytidine revealed that karyotypically abnormal HSCs persist even during complete morphologic remission and that expansion of clonotypic HSCs precedes clinical relapse. This study demonstrates that stem and progenitor cells in MDS are characterized by stage-specific expansions and contain epigenetic and genetic alterations.
Somatic mosaicism for oncogenic NRAS mutations in juvenile myelomonocytic leukemia
Authors: Doisaki Sayoko , Muramatsu Hideki , Shimada Akira , Takahashi Yoshiyuki , Mori-Ezaki Makiko , Sato Masanori , Kawaguchi Hiroyuki , Kinoshita Akitoshi , Sotomatsu Manabu , Hayashi Yasuhide , Furukawa-Hibi Yoko , Yamada Kiyofumi , Hoshino Hideaki , Kiyoi Hitoshi , Yoshida Nao , Sakaguchi Hirotoshi , Narita Atsushi , Wang Xinan , Ismael Olfat , Xu Yinyan , Nishio Nobuhiro , Tanaka Makito , Hama Asahito , Koike Kenichi , Kojima Seiji 
Published: July 2012

Abstract
Juvenile myelomonocytic leukemia (JMML) is a rare pediatric myeloid neoplasm characterized by excessive proliferation of myelomonocytic cells. Somatic mutations in genes involved in GM-CSF signal transduction, such as NRAS, KRAS, PTPN11, NF1, and CBL, have been identified in more than 70% of children with JMML. In the present study, we report 2 patients with somatic mosaicism for oncogenic NRAS mutations (G12D and G12S) associated with the development of JMML. The mutated allele frequencies quantified by pyrosequencing were various and ranged from 3%-50% in BM and other somatic cells (ie, buccal smear cells, hair bulbs, or nails). Both patients experienced spontaneous improvement of clinical symptoms and leukocytosis due to JMML without hematopoietic stem cell transplantation. These patients are the first reported to have somatic mosaicism for oncogenic NRAS mutations. The clinical course of these patients suggests that NRAS mosaicism may be associated with a mild disease phenotype in JMML.
A comparative analysis of the heterotrimeric G-protein Gα, Gβ and Gγ subunits in the wheat pathogen Stagonospora nodorum
Authors: Gummer Joel P A , Trengove Robert D , Oliver Richard P , Solomon Peter S 
Published: July 2012

Abstract

Background
It has been well established that the Gα subunit of the heterotrimeric G-protein in the wheat pathogen Stagonospora nodorum is required for a variety of phenotypes including pathogenicity, melanisation and asexual differentiation. The roles though of the Gγ and Gβ subunits though were unclear. The objective of this study was to identify and understand the role of these subunits and assess their requirement for pathogenicity and development.

Results
G-protein Gγ and Gβ subunits, named Gga1 and Gba1 respectively, were identified in the Stagonospora nodorum genome by comparative analysis with known fungal orthologues. A reverse genetics technique was used to study the role of these and revealed that the mutant strains displayed altered in vitro growth including a differential response to a variety of exogenous carbon sources. Pathogenicity assays showed that Stagonospora nodorum strains lacking Gba1 were essentially non-pathogenic whilst Gga1- impaired strains displayed significantly slower growth in planta. Subsequent sporulation assays showed that like the previously described Gα subunit mutants, both Gba1 and Gga1 were required for asexual sporulation with neither mutant strain being able to differentiate either pycnidia nor pycnidiospores under normal growth conditions. Continued incubation at 4°C was found to complement the mutation in each of the G-protein subunits with nearly wild-type levels of pycnidia recovered.

Conclusion
This study provides further evidence on the significance of cAMP-dependent signal transduction for many aspects of fungal development and pathogenicity. The observation that cold temperatures can complement the G-protein sporulation defect now provides an ideal tool by which asexual differentiation can now be dissected.
Association of estrogen receptor betavariants and serum levels of estradiol with risk of colorectal cancer: a case control study
Authors: Wu Huanlei , Xu Li , Chen Jigui , Hu Junbo , Yu Shiying , Hu Guangyuan , Huang Liu , Chen Xiaoping , Yuan Xianglin , Li Guojun 
Published: July 2012

Abstract

Background
Endogenous estrogens may play a vital role in colorectal tumorigenesis. Estrogen receptor beta is the predominant subtype which mediates the biological effect of estrogens, while loss of expression of estrogen receptor beta has been indicated as a common step in the development of colorectal cancer (CRC). Epidemiological studies have revealed several functional polymorphisms of estrogen receptor beta (ESR2) for cancer risk, but relevant study in CRC is limited, particularly in men. This study aimed to investigate the association of circulating estradiol and variations of ESR2 with CRC risk in men.

Methods
We initiated a case–control study consisting of 390 patients with CRC and 445 healthy controls in men only. We genotyped ESR2 single nucleotide polymorphisms (SNPs) rs1256049 and rs4986938 and measured serum estradiol concentration using chemilluminescence immunoassay. Multivariable logistic regression model was performed to evaluate the associations between these variables and CRC risk.

Results

ESR2 rs1256049 CT/TT genotypes were associated with reduced risk of CRC (odds ratio [OR], 0.7, 95% confidence interval [CI], 0.5–1.0), while rs4986938 CT/TT genotypes were associated with increased risk of CRC (OR, 1.5, 95% CI, 1.0–2.1). In addition, the CRC risk increased with the number of risk genotypes of these two SNPs in a dose–response manner (P

trend
, 0.003). Specifically, subjects carrying risk genotypes of both SNPs had the highest risk of CRC (OR, 2.0, 95% CI, 1.3–3.3.). Moreover, serum estradiol concentration alone was associated with risk of CRC in men (OR, 1.2, 95% CI, 1.0–1.3). However, individuals presenting both rs4986938 CT/TT genotypes and high level of serum estradiol had a high risk of CRC (OR, 2.3, 95% CI, 1.4–3.9), compared with those presenting CC genotype and low level of serum estradiol. The similar joint results were not observed for SNP rs1256049.

Conclusions
These results suggest that endogenous estrogen and genetic variations in ESR2 may individually, or more likely jointly, affect CRC risk in male Han Chinese population, while larger studies are needed to validate our findings.
Ovarian metastases resection from extragenital primary sites: outcome and prognostic factor analysis of 147 patients
Authors: Li Wenhua , Wang Huaying , Wang Jian , LV Fangfang , Zhu Xiaodong , Wang Zhonghua 
Published: July 2012

Abstract

Background
To explore the outcomes and prognostic factors of ovarian metastasectomy intervention on overall survival from extragenital primary cancer.

Methods
Patients with ovarian metastases from extragenital primary cancer confirmed by laparotomy surgery and ovarian metastases resection were retrospectively collected in a single institution during an 8-year period. A total of 147 cases were identified and primary tumor sites were colorectal region (49.0%), gastric (40.8%), breast (8.2%), biliary duct (1.4%) and liver (0.7%). The pathological and clinical features were evaluated. Patients’ outcome with different primary tumor sites and predictive factors for overall survival were also investigated by univariate and multivariate analysis.

Results
Metachronous ovarian metastasis occurred in 92 (62.6%) and synchronous in 55 (37.4%) patients. Combined metastases occurred in 40 (27.2%). Bilateral metastasis was found in 97 (66%) patients. The median ovarian metastasis tumor size was 9 cm. There were 39 (26.5%) patients with massive ascites ≥ 1000 mL on intraoperative evaluation. With a median follow-up of 48 months, the median OS after ovarian metastasectomy for all patients was 8.2 months (95% CI 7.2-9.3 months). In univariate analyses, there is significant (8.0 months vs. 41.0 months, P = 0.000) difference in OS between patients with gastrointestinal cancer origin from breast origin, and between patients with gastric origin from colorectal origin (7.4 months vs. 8.8 months, P = 0.036). In univariate analyses, synchronous metastases, locally invasion, massive intraoperative ascites (≥ 1000 mL), and combined metastasis, were identified as significant poor prognostic factors. In multivariate analyses combined metastasis (RR, 1.72; 95% CI, 1.09-2.69, P = 0.018), locally invasion (RR, 1.62; 95% CI, 1.03-2.54, P = 0.038) and massive intraoperative ascites (RR, 1.58; 95% CI, 1.02-2.49, P = 0.04) were independent factors for predicting unfavorable overall survival.

Conclusion
Ovarian metastases are more commonly originated from primary gastrointestinal tract. The prognosis of ovarian metastasis is dismal and the benefit of ovarian metastatectomy is limited. Combined metastasis outside ovaries, locally invasion and massive intraoperative ascites were independent factors for predicting unfavorable overall survival. The identification of the primary tumor is required to plan for adequate treatment for this group of patients.
Using pathology-specific laboratory profiles in Clinical Pathology to reduce inappropriate test requesting: two completed audit cycles
Authors: Baricchi Roberto , Zini Michele , Nibali Maria Grazia , Vezzosi Walter , Insegnante Vincenzo , Manfuso Clotilde , Polese Alessandra , Costoli Valmer , Spelti Antonio , Formisano Debora , Orlandini Danilo , Nicolini Fausto , Poli Antonio 
Published: July 2012

Abstract

Background
Systematic reviews have shown that, although well prepared, the Consensus Guidelines have failed to change clinical practice. In the healthcare district of Castelnovo né Monti (Reggio Emilia, Italy), it became necessary for the GPs and Clinical Pathologists to work together to jointly define laboratory profiles.

Methods
Observational study with two cycles of retrospective audit on test request forms, in a primary care setting. Objectives of the study were to develop pathology-specific laboratory profiles and to increase the number of provisional diagnoses on laboratory test request forms. A Multiprofessional Multidisciplinary Inter-hospital Work Team developed pathology-specific laboratory profiles for more effective test requesting. After 8 training sessions that used a combined strategy with multifaceted interventions, the 23 General Practitioners (GPs) in the trial district (Castelnovo nè Monti) tested the profiles; the 21 GPs in the Puianello district were the control group; all GPs in both districts participated in the trial. All laboratory tests for both healthcare districts are performed at the Laboratory located in the trial district. A baseline and a 1-year audit were performed in both districts on the GPs’ request forms.

Results
Seven pathology-specific laboratory profiles for outpatients were developed. In the year after the first audit cycle: 1) the number of tests requested in the trial district was distinctly lower than that in the previous year, with a decrease of about 5% (p < 0.001); 2) the provisional diagnosis on the request forms was 52.8% in the trial district and 42% in the control district (P < 0.001); 3) the decrease of the number of tests on each request form was much more marked in the trial district (8.73 vs. 10.77; p < 0.001).

Conclusions
The first audit cycle showed a significant decrease in the number of tests ordered only in the trial district. The combined strategy used in this study improved the prescriptive compliance of most of the GPs involved. The presence of the clinical pathologist is seen as an added value.
Identification of proteins related to the stress response in Enterococcus faecalis V583 caused by bovine bile
Authors: Bøhle Liv Anette , Færgestad Ellen M , Veiseth-Kent Eva , Steinmoen Hilde , Nes Ingolf F , Eijsink Vincent GH , Mathiesen Geir 
Published: July 2012

Abstract

Background

Enterococcus faecalis is an opportunistic pathogen and one of the most important causes of hospital infections. Bile acids are a major stress factor bacteria have to cope with in order to colonize and survive in the gastro-intestinal tract. The aim of this study was to investigate the effects of bile acids on the intracellular proteome of E. faecalis V583.

Results
The proteomes of cells challenged with 1% bile were analyzed after 20 - 120 minutes exposure, using 2D gel electrophoresis and mass spectrometry. Among the approximately 500 observed proteins, 53 unique proteins were found to be regulated in response to bile and were identified with mass spectrometry. The identified proteins belonged to nine different functional classes, including fatty acid- and phospholipid-biosynthesis, energy metabolism, and transport and binding. Proteins involved in fatty acid and phospholipid biosynthesis pathways were clearly overrepresented among the identified proteins and all were down-regulated upon exposure to bile. The proteome data correlated reasonably well with data from previous transcriptome experiments done under the same conditions, but several differences were observed.

Conclusion
The results provide an overview of potentially important proteins that E. faecalis V583 needs to regulate in order to survive and adapt to a bile-rich environment, among which are several proteins involved in fatty acid and phospholipid biosynthesis pathways. In addition, this study reveals several hypothetical proteins, which are both abundant and clearly regulated and thus stand out as targets for future studies on bile stress.
A complete response to S-1 plus cis-diamminedichloroplatinum in advanced-stage esophageal and gastric adenocarcinoma: a case report
Authors: Matsuno Yoritaka , Kochi Mitsugu , Fujii Masashi , Kanamori Noriaki , Kaiga Teruo , Mihara Yoshiaki , Funada Tomoya , Miyazaki Teruyuki , Takayama Tadatoshi 
Published: July 2012

Abstract

Background
Complete remission from advanced-stage synchronous double primary (SDP) esophageal and gastric adenocarcinoma by chemotherapy alone is rare. We report a case of advanced-stage SDP esophageal and gastric adenocarcinoma in which a complete response to treatment was obtained with S-1 and cis-diamminedichloroplatinum (CDDP).

Case presentation
The patient was a 74-year-old man referred to our hospital complaining of dysphagia. Gastrointestinal endoscopy was performed and advanced-stage SDP esophageal and gastric adenocarcinoma diagnosed. Computed tomography revealed multiple regional lymph node metastases in the mediastinum. Neoadjuvant chemotherapy with S-1 and CDDP for advanced esophageal and gastric cancer was planned. An endoscopy following two courses of chemotherapy revealed that the esophageal cancer had been replaced with a normal mucosal lesion and the gastric tumor with a scar lesion; the results of biopsies of both were negative for cancer. Computed tomography revealed that the multiple lymph node metastases had disappeared. We diagnosed a complete response to S-1 and CDDP in advanced-stage SDP esophageal and gastric cancer. The patient is still alive with no signs of recurrence 22 months after the disappearance of the original tumor and metastatic lesions without surgical treatment.

Conclusion
These results suggest that complete remission from advanced-stage esophageal and gastric cancer can be obtained with chemotherapy with S-1 plus CDDP.
Giant retroperitoneal liposarcoma
Authors: Herrera-Gómez Ángel , Ortega-Gutiérrez César , Betancourt Alejandro Mohar , Luna-Ortiz Kuauhyama 
Published: July 2012

Abstract

Background
Liposarcoma is the most frequent histopathological variety of the retroperitoneum, surgery is the gold standard for treatment.

Case presentation
We present the case of a 24-year-old male who was diagnosed with a giant retroperitoneal liposarcoma. The patient received palliative treatment due to non-resectability on the basis of chemotherapy. We decided to perform surgery after no benefit was received with systemic treatment. Complete macroscopic resection of the tumor was performed, without multi-organ resection. The patient is currently alive and disease free at 14 months of evolution.

Conclusion
Retroperitoneal liposarcomas represent a unique situation and require a more aggressive surgical approach including multiple resections for recurrences. Based on the ability of the patient to tolerate the procedure, surgery is suggested to evaluate resectability of the tumor. We must take into consideration whether prolonged survival will be attained and tumor removal will result in palliation of symptoms.
Investigations into the impact of various substrates and ZnO ultra thin seed layers prepared by atomic layer deposition on growth of ZnO nanowire array
Authors: Ding JN , Liu YB , Tan CB , Yuan NY 
Published: July 2012

Abstract
The impact of various substrates and zinc oxide (ZnO) ultra thin seed layers prepared by atomic layer deposition on the geometric morphology of subsequent ZnO nanowire arrays (NWs) fabricated by the hydrothermal method was investigated. The investigated substrates included B-doped ZnO films, indium tin oxide films, single crystal silicon (111), and glass sheets. Scanning electron microscopy and X-ray diffraction measurements revealed that the geometry and aligment of the NWs were controlled by surface topography of the substrates and thickness of the ZnO seed layers, respectively. According to atomic force microscopy data, we suggest that the substrate, fluctuate amplitude and fluctuate frequency of roughness on ZnO seed layers have a great impact on the alignment of the resulting NWs, whereas the influence of the seed layers' texture was negligible.
Lymphocyte Responses to Chymotrypsin- or Trypsin V-Digested β-Lactoglobulin in Patients with Cow's Milk Allergy
Authors: Kondo Masashi , Fukao Toshiyuki , Shinoda Shinji , Kawamoto Norio , Kaneko Hideo , Kato Zenichiro , Matsui Eiko , Teramoto Takahide , Nakano Taku , Kondo Naomi 
Published: July 2012

Abstract
Chymotrypsin- or trypsin V- (a mixture of trypsin and chymotrypsin) digested β-lactoglobulin (BLG) peptides were prepared and were confirmed to have much less immunoglobulin (lg)G and lgE reactivity compared with intact BLG by IgG inhibition enzymelinked immunosorbent assay and IgE dot blotting. The lymphocyte responses to intact BLG and these peptides were examined using peripheral blood mononuclear cells (PBMCs) from 10 patients with cow's milk allergy. The PBMCs from most patients had lower lymphocyte responses to chymotrypsin- and trypsin V-digested BLG peptides than those to intact BLG. However, PBMCs from one and two patients retained significant proliferative responses to both peptides and to only the former peptide, respectively. Interferon-c production stimulated by chymotrypsin-digested peptides was still detectable in all five patients tested. Chymotrypsindigested BLG reduced lgE reactivity but still induced some lymphocyte responses.