bluuebunny/crossref_metadata_2025_split huggingface dataset:
Full‐scale shaking table test for examination of safety and functionality of base‐isolated medical facilities
Authors: Sato Eiji , Furukawa Sachi , Kakehi Atsuo , Nakashima Masayoshi 
Published: January 2011

AbstractA series of full‐scale shaking table tests are conducted using the E‐Defense shaking table facility on a base‐isolated four‐story RC hospital structure. A variety of furniture items, medical appliances, and service utilities are placed on the hospital specimen in as realistic a manner as possible. Four ground motions are adopted, including recorded near‐fault ground motions and synthesized long‐period, long‐duration ground motions. The test results show that the base‐isolated system performed very effectively against near‐fault ground motions due to significant reduction in the floor acceleration response, and operability and functionality of the hospital service is improved significantly as compared with the case observed for the corresponding base‐fixed system. Against the long‐period ground motion, however, the hospital service is difficult to maintain, primarily because of the significant motion of furniture items and medical appliances supported by casters. Resonance accentuated large displacements and velocities on the floors of the base‐isolated system, which causes such furniture items and medical appliances to slide, sometimes more than 3 m, resulting in occasional collision with other furnitures or against the surrounding partition walls. It is notable that a key to maintaining the function of the medical facilities is to securely lock the casters of furniture and medical appliances. Copyright © 2011 John Wiley & Sons, Ltd.
Parameter Space Restrictions in State Space Models
Authors: Jun Duk Bin , Kim Dong Soo , Park Sungho , Park Myoung Hwan 
Published: January 2011

ABSTRACTThe state space model is widely used to handle time series data driven by related latent processes in many fields. In this article, we suggest a framework to examine the relationship between state space models and autoregressive integrated moving average (ARIMA) models by examining the existence and positive‐definiteness conditions implied by auto‐covariance structures. This study covers broad types of state space models frequently used in previous studies. We also suggest a simple statistical test to check whether a certain state space model is appropriate for the specific data. For illustration, we apply the suggested procedure in the analysis of the United States real gross domestic product data. Copyright © 2011 John Wiley & Sons, Ltd.
Individual voxel‐based subtype prediction can differentiate progressive supranuclear palsy from idiopathic parkinson syndrome and healthy controls
Authors: Focke Niels K. , Helms Gunther , Scheewe Sebstian , Pantel Pia M. , Bachmann Cornelius G. , Dechent Peter , Ebentheuer Jens , Mohr Alexander , Paulus Walter , Trenkwalder Claudia 
Published: January 2011

AbstractVoxel‐based morphometry (VBM) shows a differentiated pattern in patients with atypical Parkinson syndrome but so far has had little impact in individual cases. It is desirable to translate VBM findings into clinical practice and individual classification. To this end, we examined whether a support vector machine (SVM) can provide useful accuracies for the differential diagnosis. We acquired a volumetric 3D T1‐weighted MRI of 21 patients with idiopathic Parkinson syndrome (IPS), 11 multiple systems atrophy (MSA‐P) and 10 progressive supranuclear palsy (PSP), and 22 healthy controls. Images were segmented, normalized, and compared at group level with SPM8 in a classical VBM design. Next, a SVM analysis was performed on an individual basis with leave‐one‐out cross‐validation. VBM showed a strong white matter loss in the mesencephalon of patients with PSP, a putaminal grey matter loss in MSA, and a cerebellar grey matter loss in patients with PSP compared with IPS. The SVM allowed for an individual classification in PSP versus IPS with up to 96.8% accuracy with 90% sensitivity and 100% specificity. In MSA versus IPS, an accuracy of 71.9% was achieved; sensitivity, however, was low with 36.4%. Patients with IPS could not be differentiated from controls. In summary, a voxel‐based SVM analysis allows for a reliable classification of individual cases in PSP that can be directly clinically useful. For patients with MSA and IPS, further developments like quantitative MRI are needed. Hum Brain Mapp, 2011. © 2011 Wiley‐Liss, Inc.
Inhibitory effect of curcumin on oral carcinoma CAL‐27 cells via suppression of Notch‐1 and NF‐κB signaling pathways
Authors: Liao Shengkai , Xia Jun , Chen Zhiwen , Zhang Shunhua , Ahmad Aamir , Miele Lucio , Sarkar Fazlul H. , Wang Zhiwei 
Published: January 2011

AbstractCurcumin has been reported to inhibit cell growth and induce apoptosis in oral cancer cells. Although many studies have been done to uncover the mechanisms by which curcumin exerts its antitumor activity, the precise molecular mechanisms remain to be unclear. In the present study, we assessed the effects of curcumin on cell viability and apoptosis in oral cancer. For mechanistic studies, we used multiple cellular and molecular approaches such as gene transfection, real‐time RT‐PCR, Western blotting, invasion assay, and ELISA. For the first time, we found a significant reduction in cell viability in curcumin‐treated cells, which was consistent with induction of apoptosis and also associated with down‐regulation of Notch‐1 and nuclear factor‐κB (NF‐κB). Taken together, we conclude that the down‐regulation of Notch‐1 by curcumin could be an effective approach, which will cause down‐regulation of NF‐κB, resulting in the inhibition of cell growth and invasion. These results suggest that antitumor activity of curcumin is mediated through a novel mechanism involving inactivation of Notch‐1 and NF‐κB signaling pathways. J. Cell. Biochem. 112: 1055–1065, 2011. © 2011 Wiley‐Liss, Inc.
Electrophysiological characterization of potassium conductive pathways in Trypanosoma cruzi
Authors: Jimenez Veronica , Henriquez Mauricio , Galanti Norbel , Riquelme Gloria 
Published: January 2011

AbstractPotassium channels (K+ channels) are members of one of the largest and most diverse families of membrane proteins, widely described from bacteria to humans. Their functions include voltage‐membrane potential maintenance, pH and cell volume regulation, excitability, organogenesis and cell death. K+ channels are involved in sensing and responsing to environmental changes such as acidification, O2 pressure, osmolarity, and ionic concentration. Trypanosoma cruzi is a parasitic protozoan, causative agent of Chagas disease (American trypanosomiasis) an endemic pathology in Latin America, where up 200,000 new cases are reported annually. In protozoan parasites, the presence of K+ channels has been suggested, but functional direct evidence supporting this hypothesis is limited, mainly due to the difficulty of employing conventional electrophysiological methods to intact parasites. In T. cruzi, K+ conductive pathways are thought to contribute in the regulatory volume decrease observed under hyposmotic stress, the steady state pH and the compensatory response to extracellular acidification and the maintenance of plasma membrane potential. In this work we describe the isolation of plasma membrane enriched fractions from T. cruzi epimastigotes, their reconstitution into giant liposomes and the first functional characterization by patch‐clamp of K+ conductive pathways in protozoan parasites. J. Cell. Biochem. 112: 1093–1102, 2011. © 2011 Wiley‐Liss, Inc.
Cellular and muscular growth patterns during sipunculan development
Authors: Kristof Alen , Wollesen Tim , Maiorova Anastassya S. , Wanninger Andreas 
Published: January 2011

AbstractSipuncula is a lophotrochozoan taxon with annelid affinities, albeit lacking segmentation of the adult body. Here, we present data on cell proliferation and myogenesis during development of three sipunculan species, Phascolosoma agassizii, Thysanocardia nigra, and Themiste pyroides. The first anlagen of the circular body wall muscles appear simultaneously and not subsequently as in the annelids. At the same time, the rudiments of four longitudinal retractor muscles appear. This supports the notion that four introvert retractors were part of the ancestral sipunculan bodyplan. The longitudinal muscle fibers form a pattern of densely arranged fibers around the retractor muscles, indicating that the latter evolved from modified longitudinal body wall muscles. For a short time interval, the distribution of S‐phase mitotic cells shows a metameric pattern in the developing ventral nerve cord during the pelagosphera stage. This pattern disappears close to metamorphic competence. Our findings are congruent with data on sipunculan neurogenesis, as well as with recent molecular analyses that place Sipuncula within Annelida, and thus strongly support a segmental ancestry of Sipuncula. J. Exp. Zool. (Mol. Dev. Evol.) 316:227–240, 2011. © 2011 Wiley‐Liss, Inc.
Effects of hypertonic dextrose injections in the rabbit carpal tunnel
Authors: Yoshii Yuichi , Zhao Chunfeng , Schmelzer James D. , Low Phillip A. , An Kai‐Nan , Amadio Peter C. 
Published: January 2011

AbstractObjectiveThis study investigated the effects of different doses of hypertonic dextrose injection on the carpal tunnel subsynovial connective tissue (SSCT) and median nerve in a rabbit model.MethodsThirty‐eight New Zealand white rabbits weighing 4.0–4.5 kg were used. One forepaw carpal tunnel was randomly injected with one of five different treatments: saline‐single injection; saline‐two injections 1 week apart; 10% dextrose‐single injection; 20% dextrose‐single injection; or 10% dextrose‐two injections 1 week apart. Animals were sacrificed at 12 weeks after the initial injection and were evaluated by electrophysiology (EP), SSCT mechanical testing and histology.ResultsThere were significant increases in the energy absorption of the SSCT in the 10% dextrose‐double injection group compared to the saline injection groups. SSCT stiffness was also significantly increased in the 10% dextrose‐double injection group compared to the other groups. There was a significant increase in the thickness of the SSCT in the 10% dextrose‐double injection group compared to the saline‐single injection group and a significant decrease in the nerve short‐long diameter ratio in the 10% dextrose‐double injection group compared to the saline‐single injection group. There were no changes in EP among the groups.ConclusionsSSCT fibrosis is present for up to 12 weeks after dextrose injection; multiple injections have bigger effects, including what appears to be a secondary change in nerve flattening. This model may be useful to study the effects of external fibrosis on nerve morphology and physiology, such as occurs clinically in carpal tunnel syndrome. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1022–1027, 2011
Effects of adeno‐associated virus‐2‐mediated human BMP‐7 gene transfection on the phenotype of nucleus pulposus cells
Authors: Wang Chaofeng , Ruan Di‐Ke , Zhang Chao , Wang De‐Li , Xin Hongkui , Zhang Yan 
Published: January 2011

AbstractBone morphogenetic protein‐7 (BMP‐7) was found to stimulate the synthesis of proteoglycans (PGs) and collagen type II. To increase the biological function of the nucleus pulposus (NP) cells, the Ad‐hBMP‐7 vector was also successfully constructed and transfected NP cells. However, the disadvantages of adenovirus limit the usefulness of the Ad‐hBMP7 vector for clinical application. The rAAV2 vector has empirical advantages, especially for clinical use, to transfer exogenous genes into cells. The purpose of this study was to first determine whether a rAAV2‐hBMP‐7 vector could be used to transfect canine NP cells and effect on the biological functions of canine NP cells. The canine NP cells transfected by the rAAV‐BMP7 were assessed semi‐quaiitatively for BMP‐7 expression with real‐time PCR and westernbloting. Aggrecan and collagens type I and II secreted by the NP cells were qualitatively assessed at 4, 7, and 14 days post‐transfection in the transfection and control groups. We found that rAAV2 can successfully transfer the hBMP‐7 gene into canine NP cells. NP cells transfected by the rAAV‐hBMP‐7 vector express hBMP‐7 for at least 14 days. At 7 and 14 days, the expressed hBMP‐7 promotes a remarkable and significant accumulation of both proteoglycans (42% and 77% higher than non‐transfected cells) (p<0.05) and collagen type II (63% and 94% higher than non‐transfected cells) (p<0.05). Thus, we could speculate that the rAAV‐based gene delivery technique promotes the expression of proteoglycans and collagen type II of nucleus pulposus cells. Moreover, this technique may be applicable for the future treatment of degenerative disc disease. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29:838–845
Rapid attachment of adipose stromal cells on resorbable polymeric scaffolds facilitates the one‐step surgical procedure for cartilage and bone tissue engineering purposes
Authors: Jurgens Wouter J. , Kroeze Robert Jan , Bank Ruud A. , Ritt Marco J. P. F. , Helder Marco N. 
Published: January 2011

AbstractThe stromal vascular fraction (SVF) of adipose tissue provides an abundant source of mesenchymal stem cells. For clinical application, it would be beneficial to establish treatments in which SVF is obtained, seeded onto a scaffold, and returned into the patient within a single surgical procedure. In this study, we evaluated the suitability of both a macroporous poly(L‐lactide‐co‐caprolactone) and a porous collagen type I/III scaffold for this purpose. Surprisingly, cell attachment was rapid (∼10 min) and sequestered the majority of adipose stem cells, as deduced from colony‐forming unit assays. Proliferation occurred in both polymeric scaffolds. Upon chondrogenic induction, up‐regulation of chondrogenic genes, production of glycosaminoglycans, and accumulation of collagen type II was observed, indicating differentiation of scaffold‐attached SVF cells along the chondrogenic lineage. Osteogenic differentiation was achieved in both scaffold types, as visualized by up‐regulation of osteogenic genes, increase of alkaline phosphatase production over time, and accumulation of bone sialoprotein and osteonectin. In conclusion, this study identifies both poly(L‐lactide‐co‐caprolactone) and collagen type I/III as promising scaffold materials for rapid attachment of adipose stem cell‐like (stromal) cells, enhancing the development of one‐step surgical concepts for cartilage and bone tissue engineering. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29:853–860
Molecular mechanism of fatty degeneration in rotator cuff muscle with tendon rupture
Authors: Itoigawa Yoshiaki , Kishimoto Koshi N. , Sano Hirotaka , Kaneko Kazuo , Itoi Eiji 
Published: January 2011

AbstractFatty degeneration often occurs in rotator cuff muscle with tendon rupture. However, the molecular mechanism underlying this change has not been fully clarified yet. We investigated the gene expression of Wnt10b and adipogenic marker gene, PPARγ and C/EBPα in C2C12 myogenic cell line under inhibition of Wnt10b by adipogenic induction medium, isobutylmethylxanthine, dexamethasone, and insulin (MDI). The role of Wnt‐signal was confirmed by adding Lithium chloride (LiCl), which mimics Wnt signaling to the cultured cell with MDI. We also assessed the expression profiles of same genes in the rat rotator cuff tear model in vivo. MDI induced Oil red‐O staining positive adipocytes and upregulated PPARγ and C/EBPα expression. LiCl inhibited adipogenic induction of MDI. Rotator cuff muscle with tendon rupture showed positive staining for Oil red‐O. Real‐time polymerase chain reaction analyses revealed decreased expression of Wnt10b followed by increased PPARγ and C/EBPα gene expression in the supraspinatus muscle. Fatty degeneration and its molecular events were remarkably seen in the distal one‐third of the detached supraspinatus muscle versus control. Wnt signaling may regulate adipogenic differentiation both in the myoblasts in vitro and the muscle in vivo. Our results indicate that the reduction of Wnt10b in muscle with a rotator cuff tear is a key signal in fatty degeneration of the muscle. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29:861–866
Obesity augmented breast cancer risk: A potential risk factor for Indian women
Authors: Singh Alok K. , Pandey Anjita , Tewari Mallika , Pratyush Daliparthy D. , Singh Hemant K. , Pandey Hausila P. , Shukla Hari S. 
Published: January 2011

AbstractBackgroundObesity is caused by disturbances of energy balance, which is homeostasized by the physiological processes. The study aims to determine the possible impact of rising prevalence of obesity and its effect in the development of breast carcinoma (BC) in Indian population.MethodsThis study is carried out on patients (N = 358) who were diagnosed with BC and breast diseases (BD) by calculating their BMI admitted during the period of 2005 to 2009. NIH criteria were used to categorize the patients. Pathological factors of BC patient were then compared among groups.ResultThese results were indicative of significant positive association between BC risks with peri/post menopausal status, residence, diet nature, and tobacco uses. Metastases were identified more commonly with increasing weight. It was found to be independently associated with obesity I (OR = 3.103, 95% CI = 1.633–5.895) and obesity II (OR = 6.803, 95% CI = 2.415–19.162). Disease stage and cancer related mortality were significantly associated with increased BMI.ConclusionThe higher prevalence of severe obesity among Indian population was associated with BC. The only alteration apart from early diagnosis is opting for a more natural lifestyle that will affect energy equilibrium and prove to be a viable option for prevention in carcinoma of breast for better survival. J. Surg. Oncol. 2011; 103:217–222. © 2011 Wiley‐Liss, Inc.
Predictors of cure after hepatic resection of colorectal liver metastases: An analysis of actual 5‐ and 10‐year survivors
Authors: Chua Terence C. , Saxena Akshat , Chu Francis , Zhao Jing , Morris David L. 
Published: January 2011

AbstractBackgroundHepatic resection of colorectal liver metastases (CLM) is now regarded the standard of care. Evaluation of true long‐term suvivors will demonstrate the curative potential of this therapy with cure being defined as actual 10‐year survival versus a satisfactory oncological outcome of 5‐year survival. Limited data exists on outcomes of patients beyond 5 years. Studying the rates of cure and predictive factors for cure are essential to define the true benefit of this therapy.Methods Retrospective review of a prospectively maintained hepatobiliary surgical database was performed on patients who underwent hepatic resection of CLM between 1991 and 2005 with a minimum of 5‐year follow‐up. Survival was calculated from the time of surgery using the Kaplan–Meier method.Results There were 455 consecutive patients with a minimum of 5‐year follow‐up. The actuarial median overall survival was 33 months (95% CI, 29–37%), actuarial 5‐, and 10‐year survival rates were 34% and 25%, respectively. Hundred twenty four patients were identified as actual 5‐year survivors (27%) with their actuarial median overall survival being 11.1 years, actuarial 10‐year survival rate of 59%, and a median disease‐free survival of 4.9 years. Patients requiring subsequent treatment of modern systemic chemotherapy for post‐resection recurrence (P = 0.003) was a negative predictors of cure from multivariate analysis.Conclusion This study demonstrate that approximately one in three patients undergoing resection for CLM will become actual 5‐year survivors from which approximately half will go on to survive 10‐years and be cured of CLM. J. Surg. Oncol. 2011;103:796–800. © 2011 Wiley‐Liss, Inc.
Electromembrane extraction of levamisole from human biological fluids
Authors: Seidi Shahram , Yamini Yadollah , Saleh Abolfazl , Moradi Morteza 
Published: January 2011

AbstractElectromembrane extraction coupled with high‐performance liquid chromatography (HPLC) and ultraviolet (UV) detection was developed for the determination of levamisole in some human biological fluids. Levamisole migrated from 4 mL of different acidized biological matrices, through a thin layer of 2‐nitrophenyl octyl ether containing 5% tris‐(2‐ethylhexyl) phosphate immobilized in the pores of a porous hollow fiber, into a 20‐μL acidic aqueous acceptor solution present inside the lumen of the fiber. The parameters influencing electromigration were investigated and optimized. Within 15 min of operation at 200 V, levamisole was extracted from different biological fluid samples with recoveries in the range of 59–65%, which corresponded to preconcentration factors in the range of 118–130. The calibration curves showed linearity in the range of 0.5–10, 0.2–10 and 0.1–10 μg/mL for plasma, urine and saliva, respectively. Limits of detection of 0.1, 0.07 and 0.05 μg/mL and limits of quantification of 0.5, 0.2 and 0.1 μg/mL were obtained for plasma, urine and saliva, respectively. The relative standard deviations of the analysis were found to be in the range of 5.6–9.7% (n=3). Electromembrane extraction was successfully processed for determination of levamisole in plasma, urine and saliva samples.
Traumatic events and suicidality in a German adolescent community sample
Authors: Plener Paul L. , Singer Hanneke , Goldbeck Lutz 
Published: January 2011

AbstractExposure to traumatic events has been related to suicide attempts and suicidal ideation in adolescents. This study analyzes the association between suicidality and preceding traumatic life events in 665 German school students with an average age of 14.81 (SD = 0.66). Forty‐three (6.5%) students reported suicide attempts and 239 (35.9%) reported suicidal ideation. Adolescents with a history of suicide attempts reported sexual abuse 6 months before their suicidal behavior significantly more often than students with only suicidal ideation. Sexual victimization seems to be especially linked to a risk for suicide attempts during adolescence. Screening for students at risk for developing suicidality should include assessment of traumatic events.
Pulsed dye laser treatment of pigmented lesions: a randomized clinical pilot study comparison of 607‐ and 595‐nm wavelength lasers
Authors: Chern Peggy L. , Domankevitz Yacov , Ross E. Victor 
Published: January 2011

AbstractBackgroundThe 595‐nm pulsed dye laser has been used for the treatment of benign epidermal pigmented lesions (EPLs), but there is a risk of inducing undesirable purpura with treatment.ObjectiveTo compare a 607‐nm laser with a commercially‐available 595‐nm laser for the treatment of EPLs.Materials and MethodsMonte‐Carlo simulations were performed to characterize laser interaction with skin. Ten patients with EPLs were treated with a 607‐nm study prototype laser and the 595‐nm pulsed dye laser twice at 2‐ to 4‐week intervals on the left or right side on a randomized basis. Study endpoints included clearance rate of lesions, side effects immediately after treatment and at final follow‐up, and patient discomfort/pain.ResultsMonte‐Carlo simulations show that the 607‐nm is absorbed more specifically by melanin than the 595‐nm wavelength. Both lasers were effective in treatment of EPLs. The average degree of improvement overall was 41.2% with the 607‐nm laser and 40% with the 595‐nm laser. Patients reported less discomfort/pain during treatment with the 607‐nm laser.ConclusionsOur findings suggest that the 607‐nm laser is safe and at least as effective as the 595‐nm laser in treatment of EPLs. There was less patient discomfort/pain during treatment using the 607‐nm laser. Lasers Surg. Med. 42:865–869, 2010. © 2010 Wiley‐Liss, Inc.