bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a low-density polyethylene-containing passive sampler for measuring dissolved hydrophobic organic compounds in open waters
Authors: Bao Lian-Jun , Xu Shi-Ping , Liang Yan , Zeng Eddy Y. 
Published: March 2012

Abstract
A passive water sampler with low-density polyethylene (LDPE) as the sorbent phase was built and field-tested for sensing freely dissolved concentrations of hydrophobic organic compounds (HOCs) in fresh and coastal water. Based on the measured LDPE–water partition coefficients (Kpew) of 12 polycyclic aromatic hydrocarbons (PAHs) and dichlorodiphenyltrichloroethane (DDT) and its seven metabolites, the detection limits with the passive sampler containing 10-g LDPE ranged from 0.04 to 56.9 pg/L in the equilibrium sampling mode. Furthermore, the utility of the passive sampler in measuring dissolved HOC concentrations in open waters was examined through a comparison with solid-phase extraction combined with liquid–liquid extraction (SPE-LLE) and poly(dimethyl)siloxane (PDMS) coated fiber samplers. The total concentrations of PAHs (3.8–16 ng/L) obtained by the passive sampler were lower than those (87.7–115.5 ng/L) obtained through SPE-LLE. This large difference was probably attributable to slower water exchange in and out of the passive sampler as time progressed because of blockage by algae in eutrophia reservoirs and high dissolved organic carbon contents resulting in higher-than-expected PAH concentrations by SPE-LLE. Furthermore, the concentrations and compositional profiles of DDXs (sum of p,p′-DDT, p,p′-DDD, p,p′-DDE, o,p′-DDT, o,p′-DDD, o,p′-DDE, and p,p′-DDMU) at site A obtained by the passive sampler agreed with the results obtained with the PDMS-coated fibers, suggesting that the passive sampler was able to reasonably quantify dissolved HOCs in seawater. Environ. Toxicol. Chem. 2012; 31: 1012–1018. © 2012 SETAC
Cell membrane integrity and internalization of ingested TiO2 nanoparticles by digestive gland cells of a terrestrial isopod
Authors: Novak Sara , Drobne Damjana , Valant Janez , Pipan-Tkalec Živa , Pelicon Primož , Vavpetič Primož , Grlj Nataša , Falnoga Ingrid , Mazej Darja , Remškar Maja 
Published: March 2012

Abstract
The present study was motivated by the paucity of reports on cellular internalization of ingested titanium dioxide (TiO2) nanoparticles (nano-TiO2). The model invertebrate (Porcellio scaber, Isopoda, Crustacea) was exposed to food dosed with nano-TiO2 containing 100, 1,000, 3,000, or 5,000 µg nano-TiO2 per gram of food. After 14 d of exposure, the amount of Ti in the entire body was analyzed by inductively coupled plasma–mass spectrometry, and elemental analyses of tissue cross sections were performed by particle induced X-ray emission. In addition, a series of toxicological markers including feeding parameters, weight change, and survival, as well as cytotoxic effects such as digestive gland cell membrane stability, were monitored. Internalization of ingested nano-TiO2 by the isopod's digestive gland epithelial cells was shown to depend on cell membrane integrity. Cell membranes were found to be destabilized by TiO2 particles, and at higher extracellular concentrations of nano-TiO2, the nanoparticles were internalized. Environ. Toxicol. Chem. 2012; 31: 1083–1090. © 2012 SETAC
Effects of cumulative stress and impulsivity on smoking status
Authors: Ansell Emily B. , Gu Peihua , Tuit Keri , Sinha Rajita 
Published: March 2012

ObjectiveThe stress‐vulnerability model of addiction predicts that environmental factors, such as cumulative stress, will result in individual adaptations that decrease self‐control, increase impulsivity, and increase risk for addiction. Impulsivity and cumulative stress are risk factors for tobacco smoking that are rarely examined simultaneously in research.MethodsWe examined the indirect and direct effects of cumulative adversity in a community sample consisting of 291 men and women who participated in an assessment of cumulative stress, self‐reported impulsivity, and smoking history. Data were analyzed using bootstrapping techniques to estimate indirect effects of stress on smoking via impulsivity.ResultsCumulative adversity is associated with smoking status via direct effects and indirect effects through impulsivity scores. Additional models examining specific types of stress indicate contributions of traumatic stress and recent life events as well as chronic relationship stressors.ConclusionsOverall, cumulative stress is associated with increased risk of smoking via increased impulsivity and via pathways independent of impulsivity. These findings support the stress‐vulnerability model and highlight the utility of mediation models in assessing how, and for whom, cumulative stress increases risk of current cigarette smoking. Increasing self‐control is a target for interventions with individuals who have experienced cumulative adversity. Copyright © 2012 John Wiley & Sons, Ltd.
Adult attention deficit hyperactivity disorder and other psychiatric symptoms in recreational polydrug users
Authors: Parrott Andrew C. , Hatton Nicholas P. , Rowe Kathryn L. , Watts Lauren A. , Donev Rossen , Kissling Christian , Thome Johannes 
Published: March 2012

AimsPrevious research has shown that recreational drug use is associated with more psychiatric symptoms and psychobiological distress. This study investigated whether symptoms of adult attention deficit hyperactivity disorder (ADHD) were also raised in polydrug users.MethodsWe assessed a non‐clinical sample of 84 unpaid volunteers (mean age 27.5 years): n = 17 light–novice polydrug users; n = 29 moderate polydrug users; and n = 38 non‐user controls (14 non‐drug users, 24 alcohol/tobacco users). They completed the Symptom Checklist 90 (SCL‐90) self‐rating inventory for psychiatric symptoms, the Adult ADHD Self‐report Scale symptom checklist for adult ADHD, and also the questions on positive moods and sociability. Saliva samples provided a neuroendocrine cortisol measure.ResultsModerate polydrug users reported significantly higher adult ADHD symptoms and SCL‐90 psychiatric symptoms and lower sociability than non‐user controls and light polydrug users. Novice–light polydrug users did not differ from control groups on any measure. There were no significant group differences in cortisol. These findings are debated using the interactive diathesis–distress model. Psychoactive drugs can affect both mood and cognition. When taken regularly, the drug‐induced psychobiological vacillation may exacerbate prior problems with mood stability and attentional–cognitive control.ConclusionsIt is not polydrug usage per se, but rather their regular‐repeated usage, that is associated with increased signs of psychiatric and attentional–hyperactivity distress. Copyright © 2012 John Wiley & Sons, Ltd.
Cannabis‐related deficits in real‐world memory
Authors: Montgomery C. , Seddon A. L. , Fisk J. E. , Murphy P. N. , Jansari A. 
Published: March 2012

BackgroundResearch shows that cannabis users exhibit deficits in prospective memory (PM) and executive function, which persist beyond acute intoxication. However, many studies rely on self‐reports of memory failures or use laboratory‐based measures that may not mimic functional deficits in the real world. The present study aimed to assess real‐world memory functioning.MethodTwenty cannabis‐only users and 20 non‐illicit drug users were recruited. Participants completed a substance use inventory and a mood scale, followed by a non‐immersive virtual reality task assessing PM and executive functioning. The task involved the participant playing the role of an office worker for the day and performing routine office duties. A number of subscales were used to assess facets of executive function (planning, adaptive thinking, creative thinking, selection, prioritisation) and PM (time‐based, event‐based and action‐based PM).ResultsMultivariate analysis of variance revealed cannabis users performed worse overall on the task, with poor performance on the planning, time‐based PM and event‐based PM subscales. In addition, indices of cannabis (length, dose, frequency, total use) were correlated with performance on these three subscales.ConclusionsThe present study expands on previously established research, providing support for the cannabis‐related deficits in PM and executive functioning, and the role of different aspects of cannabis use in these deficits. Copyright © 2012 John Wiley & Sons, Ltd.
Parameterization and multi‐criteria calibration of a distributed storm flow model applied to a Mediterranean agricultural catchment
Authors: Hallema Dennis W. , Moussa Roger , Andrieux Patrick , Voltz Marc 
Published: March 2012

AbstractThe principal challenge in the parameterization of storm flow models for agricultural catchments with an artificial drainage network and fields with different degrees of tillage lies in the parsimonious definition of distributed model parameters in a way that reduces the number of calibration parameters to a justifiable minimum. This paper presents a comprehensive case study for the parameter estimation of a distributed storm flow model applied to an agricultural catchment (0.91 km2) in the Mediterranean region. Model parameterization was combined with procedures for multi‐criteria, multi‐storm calibration, where we automatically calibrated three parameters related to flow velocity and infiltration, and compared single and multi‐storm criteria that are based on discharge volume, peak flow, and the Nash–Sutcliffe coefficient. Multi‐storm calibration yielded a set of parameter values for the simulation batch with best multi‐storm overall performance, which are close to the median values in the pre‐calibration of individual storms. Our results suggest that flow velocities and proportionality of the channel infiltration rate do not vary significantly over the course of 11 years. Copyright © 2012 John Wiley & Sons, Ltd.
Analysis of satellite‐based and in situ hydro‐climatic data depicts water storage depletion in North China Region
Authors: Moiwo Juana Paul , Tao Fulu , Lu Wenxi 
Published: March 2012

AbstractWater storage depletion is an increasing hydrological threat to agricultural production and social stability across the globe. It is fast approaching threshold levels especially in arid/semiarid regions with low precipitation and excessive evapotranspiration (ET). This study analyses water storage dynamics in the North China Region (NCR) – an important grain‐production base in China. It uses monthly Gravity Recovery and Climate Experiment (GRACE), Global Land Data Assimilation System (GLDAS) and field‐measured precipitation data products for 2002–2009. The datasets are analysed in a basin‐scale water balance equation to determine the state of storage in the NCR study area. Based on the validated satellite‐based data products with field‐measured values, average error/bias in the datasets is <10%. The analysis also shows favourable agreements among the GRACE‐derived and flux‐based storage changes at various temporal scales. Whereas the amplitudes and phases of the precipitation and ET fluxes are largely stable for 2002–2009, those of GLDAS runoff and GRACE total water storage anomaly apparently narrow out. The linear trends in the monthly, seasonal and annual storage changes are negative for the study period, suggesting storage loss. There is an apparent seasonality of storage change in the study area; with summer storage gain, winter storage loss and an overall storage loss that is on the average of 16.8 mm/yr. Storage loss is most severe in the central floodplain region (the main irrigated production zone) of the study area. Storage depletion in this important agro‐based semi‐arid region could have negative implications for the millions of people in the region and beyond in terms of water supply, crop production, food security and social stability. Copyright © 2012 John Wiley & Sons, Ltd.
Glacier fragmentation effects on surface energy balance and runoff: field measurements and distributed modelling
Authors: Jiskoot Hester , Mueller Mark S. 
Published: March 2012

AbstractIn order to assess glacier runoff to the Upper Columbia River Basin (UCRB) and quantify energy balance effects of tributary‐trunk detachment due to recession, we used field observations to develop a distributed melt model of Shackleton Glacier, Canadian Rockies. Field data were derived from meteorological stations, ablation and snowline measurements, and weather observations between 2004 and 2010. Katabatic wind speed and direction were linked to terrain heat advection and irradiance, potentially resulting in significant cross‐glacier gradients in melt. A geographic information system‐based distributed melt model, using standard energy balance components, was developed for the 2010 melt season. Benchmark model parameterisations were derived for clear, cloudy and overcast days. Novel model parameterisations include terrain irradiance using a sky view factor and an albedo mask, and a katabatic wind ‘switch’ with valley temperature thresholds. Modelled energy balance components suggest significant sensitivities to terrain irradiance and katabatic wind, in part related to cloudiness. Glacier‐wide melt decreased by 10–15% when katabatic wind was turned off, with an interesting spatial pattern. Longwave radiation from valley walls increased local melt up to 30%, but net glacier‐wide effects were <6%. Daily glacier melt was 0.1–0.8 million m3 w.e. day−1 and peaked in early August. Net 2010 planar‐area melt was 38–50 million m3 w.e., depending on cold storage, whereas slope‐corrected‐area melt was ~4% higher. Our results indicate that katabatic wind and terrain are important in calculations of ablation in fragmenting glacier systems and that late‐summer glacier contribution to UCRB runoff at Mica Dam is ~25%. Copyright © 2012 John Wiley & Sons, Ltd.
In silico studies of potential phosphoresidues in the human nucleophosmin/B23: Its kinases and related biological processes
Authors: Ramos‐Echazábal Gioser , Chinea Glay , García‐Fernández Rossana , Pons Tirso 
Published: March 2012

AbstractHuman nucleophosmin/B23 is a phosphoprotein involved in ribosome biogenesis, centrosome duplication, cancer, and apoptosis. Its function, localization, and mobility within cells, are highly regulated by phosphorylation events. Up to 21 phosphosites of B23 have been experimentally verified even though the corresponding kinase is known only for seven of them. In this work, we predict the phosphorylation sites in human B23 using six kinase‐specific servers (KinasePhos 2.0, PredPhospho, NetPhosK 1.0, PKC Scan, pkaPS, and MetaPredPS) plus DISPHOS 1.3, which is not kinase specific. The results were integrated with information regarding 3D structure and residue conservation of B23, as well as cellular localizations, cellular processes, signaling pathways and protein–protein interaction networks involving both B23 and each predicted kinase. Thus, all 40 potential phosphosites of B23 were predicted with significant score (>0.50) as substrates of at least one of 38 kinases. Thirteen of these residues are newly proposed showing high susceptibility of phosphorylation considering their solvent accessibility. Our results also suggest that the enzymes CDKs, PKC, CK2, PLK1, and PKA could phosphorylate B23 at higher number of sites than those previously reported. Furthermore, PDK, GSK3, ATM, MAPK, PKB, and CHK1 could mediate multisite phosphorylation of B23, although they have not been verified as kinases for this protein. Finally, we suggest that B23 phosphorylation is related to cellular processes such as apoptosis, cell survival, cell proliferation, and response to DNA damage stimulus, in which these kinases are involved. These predictions could contribute to a better understanding, as well as addressing further experimental studies, of B23 phosphorylation. J. Cell. Biochem. 113: 2364–2374, 2012. © 2012 Wiley Periodicals, Inc.
The plating of rat scar myofibroblasts on matrigel unmasks a novel phenotype; the self assembly of lumen‐like structures
Authors: El‐Helou Viviane , Gosselin Hugues , Villeneuve Louis , Calderone Angelino 
Published: March 2012

AbstractDuring tissue healing, the primary role of myofibroblasts involves the synthesis and deposition of collagen. However, it has also been reported that selective populations of myofibroblasts can acquire the phenotype and/or differentiate to other cells types. The present study tested the hypothesis that myofibroblasts isolated from the scar of the ischemically damaged rat heart can recapitulate an endothelial cell‐like response when plated in a permissive in vitro environment. Scar myofibroblasts, neonatal and adult ventricular fibroblasts express smooth muscle α‐actin, collagen α1 type 1 and a panel of pro‐fibrotic and pro‐angiogenic peptide growth factor mRNAs. Myofibroblasts plated alone on matrigel led to the self assembly of lumen‐like structures whereas neonatal and adult rat ventricular fibroblasts were unresponsive. Myofibroblasts labeled with the fluorescent cell tracker CM‐DiI were injected in the viable myocardium of 3‐day post‐myocardial infarcted Sprague–Dawley rats and sacrificed 7 days later. Injected CM‐DiI‐labeled myofibroblasts were detected predominantly in the peri‐infarct/infarct region, highlighting their migration to the damaged region. However, engrafted myofibroblasts in the peri‐infarct/infarct region were unable to adopt an endothelial cell‐like phenotype or lead to the de novo formation of CM‐DiI‐labeled blood vessels. The non‐permissive nature of the infarct region may be attributed at least in part to the presence of growth‐promoting stimuli as TGF‐β and the β‐adrenergic agonist isoproterenol inhibited the self assembly of lumen‐like structures by myofibroblasts. Thus, when plated in a permissive in vitro environment, scar myofibroblasts can self assemble and form lumen‐like structures providing an additional novel phenotype distinguishing this population from normal ventricular fibroblasts. J. Cell. Biochem. 113: 2442–2450, 2012. © 2012 Wiley Periodicals, Inc.
A Structural Model for Examining how Destination Image, Perceived Value, and Service Quality Affect Destination Loyalty: a Case Study of Orlando
Authors: Kim Soon‐Ho , Holland Stephen , Han Hye‐Sook 
Published: March 2012

ABSTRACTThe primary objectives of this study were to investigate and develop a theoretical relationship among destination image, service quality and perceived value and to empirically test the constructs that are likely to affect tourist satisfaction, which in turn influence revisit intentions and word‐of‐mouth referrals. The results of empirical study indicated that destination image influences service quality and perceived value. In addition, the findings revealed that perceived value has a significant effect on satisfaction and loyalty. These research findings contribute to an extant knowledge in this domain, specifically focused on a family‐oriented destination where it was not studied yet with these relationships. Copyright © 2012 John Wiley & Sons, Ltd.
Signaling Pathways of Immobilized FGF‐2 on Silicon‐Substituted Hydroxyapatite
Authors: de la Concepción Matesanz María , Feito María José , Ramírez‐Santillán Cecilia , Lozano Rosa María , Sánchez‐Salcedo Sandra , Arcos Daniel , Vallet‐Regí María , Portolés María‐Teresa 
Published: March 2012

AbstractTherapeutic strategies for bone regeneration involve the selection of suitable biomaterials, growth factors, and cell types to mimic the cellular microenvironment where molecular and mechanical signals control the reconstruction of bone tissue. The immobilization of basic fibroblast growth factor (FGF‐2) on powdered silicon‐substituted hydroxyapatite (Si‐HA) allows to prepare a biofunctional biomaterial able to interact with bone cells in a very specific way. The biological activity of FGF‐2/Si‐HA, evaluated in Saos‐2 osteoblasts and MC3T3‐E1 preosteoblasts through the PLCγ and MAPK/ERK signal transduction pathways, shows that FGF‐2 immobilized on Si‐HA provides the right signals to cells stimulating crucial intracellular mechanisms of osteoblast proliferation and differentiation.magnified image
Some s‐numbers of embeddings of function spaces with weights of logarithmic type
Authors: Gąsiorowska Alicja , Skrzypczak Leszek 
Published: March 2012

AbstractIn this paper we deal with compact embeddings of weighted function spaces of Besov and Triebel‐Lizorkin type with weights of logarithmic growth near infinity. We obtain the exact estimates for the asymptotic behaviour of Gelfand, Kolmogorov and Weyl numbers of the embeddings. As an application we get eigenvalue estimates of some degenerate pseudodifferential operators of type. Finally we deal with the “negative spectrum” of operators Hγ = A − γV for γ → ∞, where the potential V is a positive function and A is a positive elliptic pseudodifferential operator. We concentrate on the case when the Weyl numbers and Pietsch's inequality give us better estimates then entropy numbers and Carl's inequality.
Regulation of pancreatic cancer growth by superoxide
Authors: Du Juan , Nelson Elke S. , Simons Andrean L. , Olney Kristen E. , Moser Justin C. , Schrock Hannah E. , Wagner Brett A. , Buettner Garry R. , Smith Brian J. , Teoh Melissa L.T. , Tsao Ming‐Sound , Cullen Joseph J. 
Published: March 2012

AbstractK‐ras mutations have been identified in up to 95% of pancreatic cancers, implying their critical role in the molecular pathogenesis. Expression of K‐ras oncogene in an immortalized human pancreatic ductal epithelial cell line, originally derived from normal pancreas (H6c7), induced the formation of carcinoma in mice. We hypothesized that K‐ras oncogene correlates with increased non‐mitochondrial‐generated superoxide (O), which could be involved in regulating cell growth contributing to tumor progression. In the H6c7 cell line and its derivatives, H6c7er‐Kras+ (H6c7 cells expressing K‐ras oncogene), and H6c7eR‐KrasT (tumorigenic H6c7 cells expressing K‐ras oncogene), there was an increase in hydroethidine fluorescence in cell lines that express K‐ras. Western blots and activity assays for the antioxidant enzymes that detoxify O were similar in these cell lines suggesting that the increase in hydroethidine fluorescence was not due to decreased antioxidant capacity. To determine a possible non‐mitochondrial source of the increased levels of O, Western analysis demonstrated the absence of NADPH oxidase‐2 (NOX2) in H6c7 cells but present in the H6c7 cell lines expressing K‐ras and other pancreatic cancer cell lines. Inhibition of NOX2 decreased hydroethidine fluorescence and clonogenic survival. Furthermore, in the cell lines with the K‐ras oncogene, overexpression of superoxide dismutases that detoxify non‐mitochondrial sources of O, and treatment with the small molecule O scavenger Tempol, also decreased hydroethidine fluorescence, inhibited clonogenic survival and inhibited growth of tumor xenografts. Thus, O produced by NOX2 in pancreatic cancer cells with K‐ras, may regulate pancreatic cancer cell growth. © 2012 Wiley Periodicals, Inc.