bluuebunny/crossref_metadata_2025_split huggingface dataset:
Calcium efflux of plasma membrane vesicles exposed to ELF magnetic fields—test of a nuclear magnetic resonance interaction model
Authors: Sun Wenjun J. , Mogadam Mehri Kaviani , Sommarin Marianne , Nittby Henrietta , Salford Leif G. , Persson Bertil R.R. , Eberhardt Jacob L. 
Published: April 2012

AbstractThe question whether very weak, low frequency magnetic fields can affect biological matter is still under debate. The theoretical possibility of such an interaction is often questioned and the site of interaction in the cell is unknown. In the present study, the influence of extremely weak 60 Hz magnetic fields on the transport of Ca2+ was studied in a biological system consisting of highly purified plasma membrane vesicles. We tested a newly proposed quantum mechanical model postulates that polarization of hydrogen nuclei can elicit a biological effect. Vesicles were exposed for half an hour at 32 °C and the calcium efflux was studied using radioactive 45Ca2+ as a tracer. A static magnetic field of 26 µT and time‐varying magnetic fields with a frequency of 60 Hz and amplitudes between 0.6 and 6.3 µT were used. The predictions of the model, proposed by Lednev, that at a frequency of 60 Hz the biological effect under investigation would significantly be altered at the amplitudes of 1.3 and 3.9 µT could not be confirmed. Bioelectromagnetics 33:535–542, 2012. © 2012 Wiley Periodicals, Inc.
Transcranial Doppler in a Hispanic–Mestizo population with neurological diseases: a study of sonographic window and its determinants
Authors: Brunser Alejandro M. , Silva Claudio , Cárcamo Daniel , Muñoz Paula , Hoppe Arnold , Olavarría Verónica V. , Díaz Violeta , Abarca Juan 
Published: April 2012

AbstractBetween 5% and 37% of patients are not suitable for transtemporal insonation with transcranial Doppler (TCD). This unsuitability is particularly frequent in elderly females and non‐Caucasians. We aim to evaluate TCD efficiency in a mixed Hispanic population in Santiago, Chile and to determine whether factors associated with the presence of optimal windows depend exclusively on patient‐related elements. Seven hundred forty‐nine patients were evaluated with power mode TCD. Optimal temporal windows (TWs) included detection of the middle, anterior, posterior cerebral arteries and terminal carotid. The patient's age and sex, the location of the examination, the time of day, whether the test was conducted on weekends and whether mechanical ventilation was used were recorded. Percentages of optimal windows were calculated. Examinations were deemed ideal if both TWs were optimal. TWs were obtained in 82% of cases. In univariate analyses, male sex (P < 0.001), an age below 60 years (P < 0.0001) and mechanical ventilation (P= 0.04) correlated with ideal TWs. Using logistic regression where dependent variable was a non‐ideal window only male sex odds ratio (OR) 2.3 (1.51–3.45) and age below 60 OR 13.8 (7.8–24.6) were statistically significant. Our findings indicate that Hispanic populations have detection rates for TWs similar to Europeans and are affected by patient‐related elements.
A new reduced reaction mechanism of a surrogate fuel for kerosene
Authors: Zeng Wen , Liu Jing‐chen , Chen Xiao‐Xiao , Ma Hong‐An 
Published: April 2012

AbstractThe introduction of detailed chemical reaction mechanisms for aviation fuels into complex multidimensional fluid dynamics problems is not practical at the present time. Simplified reaction mechanisms that have been thoroughly evaluated must be developed to address specific issues arising in realistic combustor configurations. A reduced chemical kinetic mechanism features 210 elemental reactions (including 92 reversible reactions and 26 irreversible reactions) and 50 species for the ignition and combustion of n‐decane was compiled and validated for a wide range of combustion regimes. Validations were performed using experimental measurements on a premixed flame of Jet‐A1, O2 and N2, stabilised at 1 atm on a flat‐flame burner, as well as from n‐decane shock‐tube ignition experiments. Numerical calculations were performed using this reduced mechanism and the detailed mechanism respectively for n‐decane surrogate fuel. The calculated values of ignition delay times at pressures of 12, 50 bar and equivalence ratio is 1.0, 2.0, respectively and the main reactants and main products mole fractions agree well with experimental data. The present study shows that this reduced mechanism for the n‐decane surrogate can be employed to predict premixed combustion of kerosene. © 2012 Canadian Society for Chemical Engineering
Lipoprotein(a), Interleukin‐10, C‐Reactive Protein, and 8‐Year Outcome After Percutaneous Coronary Intervention
Authors: Kardys Isabella , Oemrawsingh Rohit M. , Kay I. Patrick , Jones Gregory T. , McCormick Sally P.A. , Daemen Joost , Van Geuns Robert Jan , Boersma Eric , Van Domburg Ron T. , Serruys Patrick W. 
Published: April 2012

AbstractBackground:This prospective study investigated the association between preprocedural biomarker levels and incident major adverse cardiac events (MACE) in complex patients undergoing percutaneous coronary intervention (PCI) with sirolimus‐eluting stenting.Hypothesis:Lipoprotein(a) (Lp[a]), interleukin‐10 (IL‐10), and high‐sensitivity C‐reactive protein (CRP) have long‐term prognostic value in patients undergoing PCI.Methods:Between April 2002 and February 2003, 161 patients were included in the study. Blood was drawn before the procedure, and biomarkers were measured. Patients were followed‐up for MACE (death, nonfatal myocardial infarction, and repeat revascularization). Cox proportional hazard models were used to determine risk of MACE for tertiles of biomarkers. Both 1‐year and long‐term follow‐up (median, 6 years; maximum, 8 years) were evaluated.Results:Mean age was 59 years, and 68% were men. During long‐term follow‐up, 72 MACE occurred (overall crude cumulative incidence: 45% [95% confidence interval (CI): 37%‐52%]). Lp(a) was associated with a higher 1‐year risk of MACE, with an adjusted hazard ratio (HR) of 3.1 (95% CI: 1.1‐8.6) for the highest vs the lowest tertile. This association weakened and lost significance with long‐term follow‐up. IL‐10 showed a tendency toward an association with MACE. The 1‐year HR was 2.1 (95% CI: 0.92‐5.0). Long‐term follow‐up rendered a similar result. The association of CRP with MACE did not reach statistical significance at 1‐year follow‐up. However, CRP was associated with long‐term risk of MACE, with an HR of 1.9 (95% CI: 1.0‐3.5).Conclusions:In this prospective study, preprocedural Lp(a) level was associated with short‐term prognosis after PCI. The preprocedural CRP level was associated with long‐term prognosis after PCI. Clin. Cardiol. 2012 DOI: 10.1002/clc.21988The authors have no funding, financial relationships, or conflicts of interest to disclose.
Adaptation to larval crowding in Drosophila ananassae leads to the evolution of population stability
Authors: Dey Snigdhadip , Bose Joy , Joshi Amitabh 
Published: April 2012

AbstractDensity‐dependent selection is expected to lead to population stability, especially if r and K tradeoff. Yet, there is no empirical evidence of adaptation to crowding leading to the evolution of stability. We show that populations of Drosophila ananassae selected for adaptation to larval crowding have higher K and lower r, and evolve greater stability than controls. We also show that increased population growth rates at high density can enhance stability, even in the absence of a decrease in r, by ensuring that the crowding adapted populations do not fall to very low sizes. We discuss our results in the context of traits known to have diverged between the selected and control populations, and compare our results with previous work on the evolution of stability in D. melanogaster. Overall, our results suggest that density‐dependent selection may be an important factor promoting the evolution of relatively stable dynamics in natural populations.
Fine scale relationships between sex, life history, and dispersal of masu salmon
Authors: Kitanishi Shigeru , Yamamoto Toshiaki , Koizumi Itsuro , Dunham Jason B. , Higashi Seigo 
Published: April 2012

AbstractIdentifying the patterns and processes driving dispersal is critical for understanding population structure and dynamics. In many organisms, sex‐biased dispersal is related to the type of mating system. Considerably, less is known about the influence of life‐history variability on dispersal. Here we investigated patterns of dispersal in masu salmon (Oncorhynchus masou) to evaluate influences of sex and life history on dispersal. As expected, assignment tests and isolation by distance analysis revealed that dispersal of marine‐migratory masu salmon was male‐biased. However, dispersal of resident and migratory males did not follow our expectation and marine‐migratory individuals dispersed more than residents. This may be because direct competition between marine‐migratory and resident males is weak or that the cost of dispersal is smaller for marine‐migratory individuals. This study revealed that both sex and migratory life‐history influence patterns of dispersal at a local scale in masu salmon.
Circulating specific antibodies enhance systemic cross‐priming by delivery of complexed antigen to dendritic cells in vivo
Authors: van Montfoort Nadine , Mangsbo Sara M. , Camps Marcel G. M. , van Maren Wendy W. C. , Verhaart Ingrid E. C. , Waisman Ari , Drijfhout Jan Wouter , Melief Cornelis J. M. , Verbeek J. Sjef , Ossendorp Ferry 
Published: April 2012

AbstractIncreasing evidence suggests that antibodies can have stimulatory effects on T‐cell immunity. However, the contribution of circulating antigen‐specific antibodies on MHC class I cross‐priming in vivo has not been conclusively established. Here, we defined the role of circulating antibodies in cross‐presentation of antigen to CD8+ T cells. Mice with hapten‐specific circulating antibodies, but naϊve for the T‐cell antigen, were infused with haptenated antigen and CD8+ T‐cell induction was measured. Mice with circulating hapten‐specific antibodies showed significantly enhanced cross‐presentation of the injected antigen compared with mice that lacked these antibodies. The enhanced cross‐presentation in mice with circulating antigen‐specific antibodies was associated with improved antigen capture by APCs. Importantly, CD11c+ APCs were responsible for the enhanced and sustained cross‐presentation, although CD11c− APCs had initially captured a significant amount of the injected antigen. Thus, in vivo formation of antigen‐antibody immune complexes improves MHC class I cross‐presentation, and CD8+ T‐cell activation, demonstrating that humoral immunity can aid the initiation of systemic cellular immunity. These findings have important implications for the understanding of the action of therapeutic antibodies against tumor‐associated antigens intensively used in the clinic nowadays.
Stable antigen‐specific T‐cell hyporesponsiveness induced by tolerogenic dendritic cells from multiple sclerosis patients
Authors: Raϊch‐Regué Dàlia , Grau‐López Laia , Naranjo‐Gómez Mar , Ramo‐Tello Cristina , Pujol‐Borrell Ricardo , Martínez‐Cáceres Eva , Borràs Francesc E. 
Published: April 2012

AbstractMultiple sclerosis (MS) is a chronic demyelinating autoimmune disease of the central nervous system. Current therapies decrease the frequency of relapses and limit, to some extent, but do not prevent disease progression. Hence, new therapeutic approaches that modify the natural course of MSneed to be identified. Tolerance induction to self‐antigens using monocyte‐derived dendritic cells (MDDCs) is a promising therapeutic strategy in autoimmunity. In this work, we sought to generate and characterize tolerogenic MDDCs (tolDCs) from relapsing‐remitting (RR) MSpatients, loaded with myelin peptides as specific antigen, with the aim of developing immunotherapeutics for MS. MDDCs were generated from both healthy‐blood donors and RR‐MSpatients, and MDDCmaturation was induced with a proinflammatory cytokine cocktail in the absence or presence of 1α,25‐dihydroxyvitamin‐D3, a tolerogenicity‐inducing agent. tolDCs were generated from monocytes of RR‐MSpatients as efficiently as from monocytes of healthy subjects. The RR‐MStolDCs expressed a stable semimature phenotype and an antiinflammatory profile as compared with untreated MDDCs. Importantly, myelin peptide‐loaded tolDCs induced stable antigen‐specific hyporesponsiveness in myelin‐reactive T cells from RR‐MS patients. These results suggest that myelin peptide‐loaded tolDCs may be a powerful tool for inducing myelin‐specific tolerance in RR‐MS patients.
T cells interact with T cells via CD40‐CD154 to promote autoimmunity in type 1 diabetes
Authors: Baker Rocky L. , Mallevaey Thierry , Gapin Laurent , Haskins Kathryn 
Published: April 2012

AbstractWe have investigated the role of CD40 signaling in islet‐reactive, diabetogenic CD4+ Th1 T‐cell clones. Using multispectral flow cytometry, we showed that CD40 and CD154 are co‐expressed and form complexes on the surface of activated T cells. We also demonstrate that activated Tcells can transactivate CD4+CD40+ T cells through the CD40‐CD154 pathway. To investigate the role of CD40 signaling on Th1 cells, we used the diabetogenic clone BDC‐5.2.9 retrovirally transduced with a truncated form of the CD40 molecule to produce a CD40 dominant‐negative T‐cell clone. Upon challenge with antigen in vitro, the production of IFN‐&&num;x003B3; by BDC‐5.2.9 CD40DN was greatly reduced and, in vivo, the dominant‐negative variant was unable to induce diabetes. Transduction with the CD40DN vector was also effective in preventing transfer of disease by primary NOD CD4+ T cells. Ex vivo analysis of pancreatic infiltrates after transfer of BDC‐5.2.9 CD40DN cells revealed an overall reduction of cell numbers and cytokine production by both T cells and macrophages. These data indicate that CD40 is an important signaling molecule on autoreactive CD4+ T cells and contributes to their pathogenic effector function.
An additional piece in the puzzle of neutrophil‐derived IL‐1β: The NLRP3 inflammasome
Authors: Tamassia Nicola , Zimmermann Maili , Cassatella Marco A. 
Published: April 2012

AbstractThe notion that neutrophils play a pivotal role in orchestrating ongoing inflammatory immune responses has been bolstered by several fairly newly described effector mechanisms, particularly their capacity to serve as a source of cytokines. This frequently neglected phenomenon is acquiring more and more credit and, as a result, our understanding of the molecular basis of neutrophil‐derived cytokines has grown tremendously in the past 20 years. It is now clear that cytokine secretion by neutrophils is controlled by sophisticated regulatory mechanisms. In this issue of the European Journal of Immunology, Mankan et al. (Eur. J. Immunol. 42: 710‐715) further extend our knowledge by reappraising the role of the inflammasome pathway, specifically the NLRP3 sensor, in the secretion of mature IL‐1β by murine neutrophils. Accordingly, Mankan et al. (Eur. J. Immunol. 42: 710‐715) identify the neutrophil expression of the NLRP3 inflammasome complex, and by using specific knockout mice, they also show that, in LPS‐primed neutrophils, the NLRP3/ASC/caspase‐1 axis plays a nonredundant role for IL‐1β processing in response to typical NLRP3 inflammasome stimuli.
Mutation analysis of ASXL1, CBL, DNMT3A, IDH1, IDH2, JAK2, MPL, NF1, SF3B1, SUZ12, and TET2 in myeloproliferative neoplasms
Authors: Brecqueville Mandy , Rey Jérôme , Bertucci François , Coppin Emilie , Finetti Pascal , Carbuccia Nadine , Cervera Nathalie , Gelsi‐Boyer Véronique , Arnoulet Christine , Gisserot Olivier , Verrot Denis , Slama Borhane , Vey Norbert , Mozziconacci Marie‐Joelle , Birnbaum Daniel , Murati Anne 
Published: April 2012

AbstractSince the discovery of the JAK2V617F tyrosine kinase‐activating mutation several genes have been found mutated in nonchronic myeloid leukemia (CML) myeloproliferative neoplasms (MPNs), which mainly comprise three subtypes of “classic” MPNs; polycythemia vera (PV), essential thrombocythemia (ET), and myelofibrosis (MF). We searched for mutations in ASXL1, CBL, DNMT3A, IDH1, IDH2, JAK2, MPL, NF1, SF3B1, SUZ12, and TET2 genes in 149 non‐CML MPNs, including 127 “classic” MPNs cases. JAK2 was mutated in 100% PV, 66% ET and 68% MF. We found a high incidence of ASXL1 mutation in MF patients (20%) and a low incidence in PV (7%) and ET (4%) patients. Mutations in the other genes were rare (CBL, DNMT3A, IDH2, MPL, SF3B1, SUZ12, NF1) or absent (IDH1). © 2012 Wiley Periodicals, Inc.
SNP array analysis of acute promyelocytic leukemia may be of prognostic relevance and identifies a potential high risk group with recurrent deletions on chromosomal subband 1q31.3
Authors: Nowak Daniel , Klaumuenzer Marion , Hanfstein Benjamin , Mossner Maximilian , Nolte Florian , Nowak Verena , Oblaender Julia , Hecht Anna , Hütter Gero , Ogawa Seishi , Kohlmann Alexander , Haferlach Claudia , Schlegelberger Brigitte , Braess Jan , Seifarth Wolfgang , Fabarius Alice , Erben Philipp , Saussele Susanne , Müller Martin C. , Reiter Andreas , Buechner Thomas , Weiss Christel , Hofmann Wolf‐Karsten , Lengfelder Eva 
Published: April 2012

AbstractTo search for new copy number alterations (CNAs) in acute promyelocytic leukemia (APL), we analyzed DNA from leukemic blasts of 93 acute promyelocytic leukemia (APL) patients with Genome‐Wide SNP 6.0 arrays (SNP‐A). We identified 259 CNAs consisting of 170 heterozygous deletions, 82 amplifications, and 7 regions of copy number neutral loss of heterozygosity. One of the most common CNAs was a deletion on chromosomal subband 1q31.3 in 13 of 93 (14%) patients encompassing the coding regions for the microRNAs mir181a1/b1. In multivariable analysis with the covariates age, white blood cell count, platelet count, and FLT3‐ITD/FLT3 D835 mutations we found that after adjustment for patients' age (P < 0.0001), patients with 2 or more CNAs detected by SNP‐A had a higher risk of death (hazard ratio = 5.942, P = 0.0015) than patients with 0 or 1 CNA. Deletions of 1q31.3 were associated with a higher number of CNAs (median 2 vs. 8, P < 0.0001) and were a strong independent prognostic factor for an increased risk of relapse (hazard ratio = 28.9, P = 0.0031). This study presents a comprehensive assessment of new CNAs as pathomechanistically relevant targets and possible prognostic factors which could refine risk stratification of APL. © 2012 Wiley Periodicals, Inc.
A Native American Voice in Multicultural Psychology: Finding Healing in an Interpersonal Tapestry
Authors: Robbins Rockey R. 
Published: April 2012

The tribal‐cultural milieu in which the author was raised incited awareness that Native American people live in a great tapestry of life where everything and everyone is interconnected. It is an awareness that cannot be taught through lectures or books. It is an awareness of connectedness that Native American people had in early childhood but was trained out of them as they were integrated into Western society.El entorno tribal‐cultural en el que creció el autor fomentó su conciencia de que el pueblo Americano Nativo vive en un gran tapiz de vida donde todo y todos los humanos están interconectados. Es una conciencia que no puede ser enseñada en clases o libros. Es una conciencia de conexión que los individuos Americanos Nativos tenían durante su infancia temprana, pero fueron adiestrados hasta perderla al ser integrados en la sociedad occidental.
Cultural Validity of the Minnesota Multiphasic Personality Inventory–2 Empirical Correlates: Is This the Best We Can Do?
Authors: Hill Jill S. , Robbins Rockey R. , Pace Terry M. 
Published: April 2012

This article critically reviews empirical correlates of the Minnesota Multiphasic Personality Inventory–2 (MMPI‐2; Butcher, Dahlstrom, Graham, Tellegen, & Kaemmer, 1989), based on several validation studies conducted with different racial, ethnic, and cultural groups, and argues for the urgent development of culturally derived measures that would illuminate culturally relevant empirical correlates for use in validating the MMPI‐2.Este artículo examina críticamente correlatos empíricos del Test de Personalidad Multifásico de Minnesota‐2 (MMPI‐2, por sus siglas en inglés), basándose en varios estudios de validación conducidos con distintos grupos raciales, étnicos y culturales, y razona a favor del desarrollo urgente de medidas derivadas culturalmente que puedan ilustrar correlatos empíricos culturalmente relevantes para su uso en la validación del MMPI‐2.
Correlates and Predictors of Binge Eating Among Native American Women
Authors: Clark Julie Dorton , Winterowd Carrie 
Published: April 2012

Among a sample of Native American/American Indian women, this study explored the linear relationship of historical loss, acculturation, racism, and emotional distress to binge‐eating behavior and level of obesity as determined by body mass index. Emotional distress, experiences of racism, and feelings about historical loss were significantly related to binge‐eating behaviors, with emotional distress being the most significant predictor.Este estudio exploró entre Indios Americanos la relación linear de la pérdida histórica, la aculturación, el racismo y la angustia emocional con el comportamiento de atracones compulsivos y la obesidad, determinada por el índice de masa corporal. La angustia emocional, las experiencias de racismo y los sentimientos de pérdida histórica estuvieron significativamente relacionados con los comportamientos de atracones compulsivos, siendo la angustia emocional el vaticinador más significativo.
Maintenance plasma exchange therapy for steroid‐refractory neuromyelitis optica
Authors: Khatri Bhupendra O. , Kramer John , Dukic Mary , Palencia Mauricio , Verre William 
Published: April 2012

AbstractNeuromyelitis optica (NMO) is a severe inflammatory demyelinating disease of the central nervous system with exacerbations involving the optic nerves, spinal cord, or both. This study explores the utility of maintenance plasma exchange (mTPE) as a therapy in patients with relapsing, corticosteroid‐refractory, NMO. This retrospective case series presents data on patients who were diagnosed with NMO using currently accepted criteria. These patients were refractory to high‐dose corticosteroids and received mTPE. Seven patients met the criteria for NMO diagnosis and all were positive for antibodies against aquaporin‐4. Over a mean of 7.1 years (range, 2–16), these patients received between 21 and 154 TPE treatments (mean, 76). Although treated with mTPE, five out of the seven patients improved by more than one point on the Expanded Disability Status Scale. Interruption in mTPE in five patients resulted in clinical worsening. When mTPE was restarted in three out of the five patients who experienced a cessation of mTPE, these patients either stabilized or improved. The two patients who did not restart the mTPE protocol died. Patients treated with mTPE also experienced a reduction in the number of NMO exacerbations. Finally, stabilization of the retinal nerve fiber layer thickness was observed while on mTPE. In this preliminary study, mTPE appeared safe and may bring about improvement in disability and sustained stabilization of the clinical course in patients with steroid‐refractory relapsing forms of NMO. J. Clin. Apheresis, 2012. © 2012 Wiley Periodicals, Inc.