bluuebunny/crossref_metadata_2025_split huggingface dataset:
Staphylococcus warneridampens SUMOylation and promotes intestinal inflammation
Authors: Loison Léa , Huré Marion , Lefranc Benjamin , Leprince Jérôme , Coëffier Moïse , Ribet David 
Published: April 2024

AbstractGut bacteria play key roles in intestinal physiology, via the secretion of diversified bacterial effectors. Many of these effectors remodel the host proteome, either by altering transcription or by regulating protein post-translational modifications. SUMOylation, a ubiquitin-like post-translational modification playing key roles in intestinal physiology, is a target of gut bacteria. Mutualistic gut bacteria can promote SUMOylation, via the production of short- or branched-chain fatty acids (SCFA/BCFA). In contrast, several pathogenic bacteria were shown to dampen SUMOylation in order to promote infection. Here, we challenge this dichotomic vision by showing thatStaphylococcus warneri, a non-pathogenic bacterium of the human gut microbiota, decreases SUMOylation in intestinal cells. We identified that Warnericin RK, a hemolytic toxin secreted byS. warneri, targets key components of the host SUMOylation machinery, leading to the loss of SUMO-conjugated proteins. We further demonstrate that the dampening of SUMOylation triggered by Warnericin RK promotes inflammation, and, more particularly, TNFα-dependent intestinal inflammatory responses.Together, these results highlight the diversity of mechanisms used by non-pathogenic bacteria from the gut microbiota to manipulate host SUMOylation. They further highlight that changes in gut microbiota composition may impact intestinal inflammation, by changing the equilibrium between bacterial effectors promoting or dampening SUMOylation.
EnteropathogenicProvidencia alcalifaciens: A subgroup ofP. alcalifaciensthat causes diarrhea
Authors: Bulach Dieter , Carter Glen P. , Albert M. John 
Published: April 2024

AbstractEven thoughProvidencia alcalifaciensis considered as a normal flora of the large intestine, there are reports of it causing diarrhea. In a previous study, a strain, 2939/90 obtained as a pure stool culture from a dead diarrheal patient was shown to cause invasion and actin condensation in mammalian cells, and diarrhea in a rabbit model. In a subsequent study, four TnphoAmutants of 2939/90 produced negligible invasion and actin condensation in mammalian cells. In the present study, the parent strain was sequenced by short-read and long-read sequencing, and the mutants by short read sequencing. In all four mutants, a TnphoAinsertion was detected in the type three secretion system (T3SS) locus present on the largest of four plasmids (p2939_90_1) and not in a seemingly independent, functional T3SS locus on the chromosome. A survey of 52 genomes ofP. alcalifaciensavailable in the public database identified the chromosomal T3SS locus in all strains, including bothP. alcalifaciensgenomic clades that we have classified as (group A) and (group B); a highly related gene layout and gene synteny flanking the locus suggested that these chromosomal loci are orthologous. There is a low sequence similarity between the chromosomal and plasmid-borne T3SS; a survey of plasmid T3SS showed its presence in only 21 of 52 genomes and mostly in group A genomes. Group A included several isolates from an outbreak of haemorrhagic diarrhea in dogs. Using prediction software (EffectiveDB), we detected several known and unknown effectors flanking the plasmid T3SS locus. The observation that TnphoAinsertion only in the plasmid T3SS locus affected the invasion phenotype suggested that this locus is critical for causation of diarrhea. This leads us to conclude that a subgroup ofP. alcalifaciensthat possesses this plasmid-borne T3SS locus (in the case of strain, 2939/90) can cause diarrheal disease. We name this subgroup as enteropathogenicP. alcalifaciens(EPA). EPA should be included in future studies of etiology of diarrhea. A unique sequence that may be present in the T3SS locus in the plasmid may be investigated as a marker in a simple molecular test for diagnosis of EPA.
Pervasive translation of the downstream ORF from bicistronic mRNAs by human cells: impact of the upstream ORF codon usage and splicing
Authors: Paget-Bailly Philippe , Helpiquet Alexandre , Decourcelle Mathilde , Bories Roxane , Bravo Ignacio G. 
Published: April 2024

AbstractIn this work, we present a straightforward model to study gene expression regulation from virus-like bicistronic mRNAs in human cells, composed by a shortshbleupstream ORF and a reporteregfpdownstream ORF. We have engineered thirteen synonymous versions of theshbleORF to explore a large parameter space in compositional and folding features, as well as differences in propensity to undergo splicing. Our experimental model focuses on the control and modulation ofshbletranslation elongation and their effects on downstream translation: i) regarding translation initiation, all constructs share identical 5’UTR, ribosomal binding site andshblestart codon context; and ii) regardingegfptranslation, all constructs share identicalshblestop codon context, spacer betweenshbleandegfpand the fullegfpsequence. Our results show first that the human translation machinery can translate the downstream ORF in bicistronic mRNAs, independently of the characteristics of the upstream ORF. Even if GFP protein levels are hundreds of times lower than SHBLE protein levels, our results challenge the textbook interpretation positing that the eukaryotic translation machinery does not handle the downstream ORF in bicistronic transcripts. Second, we show that synonymous recoding of the upstream ORF determines its own translation efficiency, can uncover cryptic splice signals, and largely conditions the probability of translation from the downstream ORF. Our results are consistent with a leaky scanning mechanism at play for downstream ORF translation from bicistronic mRNAs in human cells.
Long range segmentation of prokaryotic genomes by gene age and functionality
Authors: Wolf Yuri I. , Schurov Ilya V. , Makarova Kira S. , Katsnelson Mikhail I. , Koonin Eugene V. 
Published: April 2024

AbstractBacterial and archaeal genomes encompass numerous operons that typically consist of two to five genes. On larger scales, however, gene order is poorly conserved through the evolution of prokaryotes. Nevertheless, non-random localization of different classes of genes on prokaryotic chromosomes could reflect important functional and evolutionary constraints. We explored the patterns of genomic localization of evolutionarily conserved (ancient) and variable (young) genes across the diversity of bacteria and archaea. Nearly all bacterial and archaeal chromosomes were found to encompass large segments of 100-300 kilobases that were significantly enriched in either ancient or young genes. Similar clustering of genes with lethal knockout phenotype (essential genes) was observed as well. Mathematical modeling of genome evolution suggests that this long-range gene clustering in prokaryotic chromosomes reflects perpetual genome rearrangement driven by a combination of selective and neutral processes rather than evolutionary conservation.
Exclusion of condensin I from the nucleus during prophase coordinates mitotic chromosome reorganization to complete sister chromatid resolution
Authors: Eykelenboom John K. , Gierliński Marek , Yue Zuojun , Tanaka Tomoyuki U. 
Published: April 2024

AbstractDuring early mitosis, chromosomes are reorganized from their relatively decompacted interphase state into characteristic mitotic rod-shaped structures. This process is important to allow correct segregation of newly replicated sister chromatids to the opposite spindle poles during anaphase. To facilitate mitotic chromosome organization, two protein complexes named condensin I and condensin II play crucial roles. Condensin II is particularly important for achieving sister chromatid separation (resolution) whilst condensin I is required for chromosome condensation (compaction). Although sister chromatid resolution occurs 15-20 min earlier before chromosome compaction, it is not yet clear how these events are temporally coordinated or whether this temporal coordination is important to ensure chromosome segregation later in mitosis. One hypothesis is that the temporal coordination is achieved through different subcellular localisation of two condensin complexes; whilst condensin II localizes in the nucleus, condensin I is restricted to the cytoplasm, during interphase and prophase. In this study we tested this hypothesis by engineering the localization of condensin I to the nucleus. We monitored sister chromatid resolution and chromosome compaction by real-time imaging that visualized selected neighboring chromosome loci. We found that localization of condensin I to the nucleus led to precocious chromosome compaction during prophase with a similar timing to sister chromatid resolution. We also monitored later stages of mitosis and found that cells expressing nuclear condensin I subsequently exhibited frequent chromosome mis-segregation in anaphase. Therein, the majority of mis-segregated chromosomes consisted of lagging chromosomes involving both sister chromatids. This suggests that the temporal control of mitotic chromosome reorganization is crucial for high-fidelity chromosome segregation. In conclusion, the exclusion of condensin I from the nucleus during prophase delays chromosome compaction and allows condensin II to complete sister chromatid resolution, which ensures correct chromosome segregation later in mitosis.
Who can afford to blame? Sender effects in blame-shifting crisis communications
Authors: Antonetti Paolo , Baghi Ilaria 
Published: April 2024

Purpose
When companies face a crisis, they sometimes deliver blame-shifting communications, trying to shift blame onto another actor to protect their reputation. While previous research has considered how different features of the message affect its persuasiveness, little is known about whether specific senders can blame more effectively. This paper aims to contribute to research in this domain through an investigation of the sender’s social perception as a critical moderator to the persuasiveness of blame shifting.

Design/methodology/approach
The authors conduct four between-subjects scenario experiments to test the research hypotheses. In each experiment, participants are presented with a realistic crisis scenario and the crisis communications delivered by the company. The authors assess the extent to which perceptions of the sender influence the message’s ability to reduce negative word-of-mouth intentions and to increase purchase intentions.

Findings
The authors show that blame shifting is more likely to be effective when deployed by senders that are small (Study 1) or have a positive CSR track record (Study 2). Furthermore, The authors find that even large senders can successfully deploy blame shifting if they can benefit from being known for their CSR programs (Study 3). Finally, the authors show that the effect of blame shifting depends on the receiver’s level of concern about the crisis: stakeholders significantly concerned by the crisis reject blame-shifting communications (Study 4).

Research limitations/implications
Further research should examine the impact of information about brand competence on blame-shifting effectiveness. Further research is also needed to explore sender effects for other defensive crisis communication strategies such as denial or the use of excuses or justifications.

Practical implications
The study offers critical information for marketers considering the use of defensive crisis communications strategies such as blame shifting.

Originality/value
The study extends the understanding of how sender effects influence blame-shifting communications. The analysis allows us to clarify why this strategy is effective for certain senders and certain receivers while, for others, it tends to backfire. Blame shifting backfires for large senders unless they can boast a strong CSR record.
The influence of ChatGPT on digital learning: experience among university students
Authors: Komba Mercy Mlay 
Published: April 2024

Purpose
This study aims to investigate the influence of ChatGPT, an AI-based chatbot, on the digital learning experience of students at Mzumbe University.

Design/methodology/approach
This study adopted a qualitative research design to gather in-depth insights from participants. Semi-structured interviews and an analysis of previous chat content were used as primary sources of data. Thematic analysis was used to analyze the qualitative data, allowing for the exploration of participants’ perspectives, experiences and opinions regarding the integration of ChatGPT into the learning process.

Findings
The results of the study demonstrated that ChatGPT is widely used in educational contexts and has a positive influence on students’ study habits, academic performance, and understanding of course material. Students appreciated the system’s simplicity, tailored instructions, and the promptness and accuracy of the responses. Despite the possibility of isolated mistakes.

Research limitations/implications
It is important to recognize the limitations of this study. First, the sample size was small, limiting the broad application of the results. Second, this study’s narrow emphasis on students at Mzumbe University limits its applicability in other situations. Furthermore, depending on self-reported experiences, biases, such as individual interpretation or recollection bias, can occur.

Practical implications
Educators can maximize ChatGPT in the classroom by using study insights. Its advantages, such as effectiveness and enhanced performance, highlight the possibility for student-centered learning. Practitioners are guided by their awareness of problems, such as probable errors. Constant updates guarantee ChatGPT’s applicability and provide educators with useful advice.

Social implications
Peer impact is highlighted in this study concerning social factors on the adoption of AI in education. Resolving issues preserves public confidence. Views influence public opinion and direct policymakers in discussions about safe AI use. It influences public attitudes while navigating the ethical integration of AI.

Originality/value
This study offers insightful information about the impact of ChatGPT on digital learning in Tanzania’s higher education. It makes innovative research contributions that enhance educational practices and emphasizes the advantages, difficulties and demands of responsible usage in the context of AI-based chatbots.
Are robots stealing our jobs? Examining robot-phobia as a job stressor in the hospitality workplace
Authors: Chen Chun-Chu (Bamboo) , Cai Ruiying 
Published: April 2024

Purpose
The purpose of this study is to explore robot-phobia as a source of occupational stress among hospitality employees in the context of increasing robotization in the industry.

Design/methodology/approach
The study sampled 321 lodging employees and 308 food service employees in the USA. An online panel company recruited the participants and administered an online survey. The study used various analytical methods, including structural modeling, t-tests and multi-group analyses.

Findings
The study results reveal that hospitality workers experience robot-phobia regardless of their sector or position. Robot-phobia causes job insecurity and stress, which increases turnover intention. These negative outcomes are more pronounced for those who interact more frequently with robots.

Practical implications
The study findings suggest that hospitality workers fear being replaced by robots in the near future. Therefore, hospitality organizations should offer adequate training and education on the advantages and drawbacks of robots and establish a supportive and collaborative work environment that values human–robot interaction.

Originality/value
This study offers new insights regarding human–robot interaction from the employee perspective by introducing the concept of robot-phobia in the hospitality workplace. A comprehensive picture of how hospitality employees confront the increasing presence of robots is provided in this study.
How overloaded employees can use resilience and forgiveness resources to overcome dissatisfaction and maintain their knowledge-sharing efforts
Authors: De Clercq Dirk , Pereira Renato 
Published: April 2024

Purpose
Drawing on conservation of resources theory, this study aims to examine how employees’ experiences of excessive workloads may direct them away from efforts to share knowledge with other organizational members, as well as the circumstances in which this process is more or less likely. To untangle the process, the authors predict a mediating role of job dissatisfaction and moderating roles of two complementary resources that help employees cope with failure: resilience as a personal resource and organizational forgiveness as an organizational resource.

Design/methodology/approach
Survey data were gathered from employees of an organization that operates in the construction retail sector. The Process macro provides an empirical test of the moderated mediation dynamic that underpins the proposed conceptual framework.

Findings
The statistical findings affirm that an important channel through which employees’ perceptions that their work demands are unreasonable escalate into a diminished propensity to share knowledge is their lack of enthusiasm about their jobs. Their ability to recover from challenging work situations and their beliefs that the organization does not hold grudges against people who commit mistakes both mitigate this harmful effect.

Practical implications
For organizational practitioners, this research shows that when employees feel frustrated about extreme work pressures, the resource-draining situation may escalate into diminished knowledge sharing, which might inadvertently undermine their ability to receive valuable feedback for dealing with the challenges. From a positive perspective, individual resilience and organizational forgiveness represent resources that can protect employees against this negative spiral.

Originality/value
This study explicates an unexplored harmful effect of strenuous workloads on knowledge sharing, which is explained by employees’ beliefs that their organization fails to provide satisfactory job experiences. This effect also is mitigated to the extent that employees can draw from valuable personal and organizational resources.
Netnography: a research method to study supply chain members' interactions in online communities
Authors: Rynarzewska Ania Izabela , Giunipero Larry 
Published: April 2024

PurposeThe objective of this paper is to further the understanding of netnography as a research method for supply chain academics. Netnography is a method for gathering and gaining insight from industry-specific online communities. We prescribe that viewing netnography through the lens of the supply chain will permit researchers to explore, discover, understand, describe or report concepts or phenomena that have previously been studied via survey research or quantitative modeling.Design/methodology/approachTo introduce netnography to supply chain research, we propose a framework to guide how netnography can be adopted and used. Definitions and directions are provided, highlighting some of the practices within netnographic research.FindingsNetnography provides the researcher with another avenue to pursue answers to research questions, either alone or in conjunction with the dominant methods of survey research and quantitative modeling. It provides another tool in the researchers’ toolbox to engage practitioners in the field.Originality/valueThe development of netnography as a research method is associated with Robert Kozinets. He developed the method to study online communities in consumer behavior. We justify why this method can be applied to supply chain research, how to collect data and provide research examples of its use. This technique has room to grow as a supply chain research method.
Transfer film formation and dissipation and its effect on friction coefficient in NAO friction materials containing FeS2 lubricant
Authors: Go Sangryul 
Published: April 2024

Purpose
The purpose of this study is to investigate the accumulation process of transfer film formation and dissipation and its effect on friction coefficients in non asbestos organic friction materials with various lubricant FeS2 contents.

Design/methodology/approach
In total, 2.5%, 5% and 10% FeS2 were added as lubricating components to the friction materials. Friction tests composed of two stages were conducted for these friction materials, and the friction surfaces of the counterpart discs were examined using scanning electron microscopy.

Findings
The transfer film formation reduced the friction coefficients, and the transfer film dissipation influenced the recovery of the friction coefficients. The effect of a high content of FeS2 was to promote the transfer film formation at high temperatures and to hinder the transfer film dissipation at low temperatures, thus resulting in a decrease in the friction coefficients at high temperatures together with recovery retardation at low temperatures.

Originality/value
FeS2 contributed to the transfer film formation at high temperatures in the fade test but hindered the transfer film removal in the recovery test, resulting in the retardation of friction coefficient recovery. The mechanism by which the FeS2 lubricant component affected the transfer film formation and dissipation was analyzed and attributed to the different levels of FeS2 pyrolysis at different temperature levels.
Educational trajectories for residential care experienced young people are complex. A lived experience perspective from a PhD study in Scotland
Authors: Whitelaw Ruby Valerie 
Published: April 2024

Purpose
Research highlights that residential care experienced children and young people in Scotland have poorer educational outcomes than their peers within the wider population. Despite this, poor educational attainment is not inevitable, and further research is needed to increase the understanding of long-term trajectories. This paper aims to address a gap in contemporary literature that is of benefit to practitioners, academics and policymakers. Despite experiencing adversity, attachment, separation and loss, school attainment data on leaving care only reflects part of the educational journey.

Design/methodology/approach
Using a mixed methodology and social constructionist theoretical framework, a practitioner-led PhD study gathered data from questionnaires and qualitative information from 13 semi-structured interviews with young people who had experienced residential care in Scotland. Recruitment was through a gatekeeper within a national third-sector organisation. The educational trajectories for young people with experience of residential care in Scotland are complex. A lived experience perspective from a PhD study illustrates that statistical data only captures part of the journey and the author needs to reconsider how success is measured.

Findings
Of the 13 participants in the study, 12 achieved success educationally, although for the majority of those interviewed, attainment continued after leaving compulsory education. Barriers to greater success included placement uncertainty and movement, stigma, low expectations, pressure to not become a statistic, procedural obstacles and inconsistency or poor relationships.

Research limitations/implications
Supportive relationships and stable placements can create circumstances conducive to effective learning, but evidence reflects that support is necessary throughout the life course if children, young people and adults with care experience are to reach their full academic potential.

Originality/value
Research into the educational outcomes for those with experience of residential care in Scotland is limited. This paper, from a PhD, provides lived experience accounts from a practitioner-led study.