bluuebunny/crossref_metadata_2025_split huggingface dataset:
ChemInform Abstract: Preparation and Screening of Glycopeptide Libraries
Authors: St. Hilaire Phaedria M. , Halkes Koen M. , Meldal Morten 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Fluorination of P‐Borophenylalanine
Authors: Yoshino Kazuo , Inamoto Yoshiro , Ido Tatsuo , Kakihana Hidetake , Ichihashi Masamitsu , Mishima Yutaka 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Concise Synthesis of Enantiomerically Pure L‐4(‐ Borophenyl)alanine from L‐Tyrosine
Authors: Nakamura Hiroyuki , Fujiwara Masaru , Yamamoto Yoshinori 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Stereoselective Synthesis of β‐Mannoglycosides
Authors: Ito Yukishige , Ohnishi Yuki 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Glycosyl Halides in Oligosaccharide Synthesis
Authors: Nitz Mark , Bundle David R. 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Properties of Oligosaccharides
Authors: Witczak Zbigniew J. 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Non‐Sugar Glycomimetics
Authors: Wessel Hans Peter 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Towards Highly Air‐Stable Phosphorus Ligands and Metal‐Catalyst Precursors for Cross‐Coupling Reactions with Unactivated Aryl Chlorides
Authors: Lagona Jason , Jarvis Bruce B. 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Solvias Josiphos Ligands: From Discovery to Technical Applications
Authors: Blaser Hans‐Ulrich , Brieden Walter , Pugin Benoit , Spindler Felix , Studer Martin , Togni Antonio 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Electron‐Transfer Processes in Mononuclear Polypyridine Metal Complexes
Authors: Vlcek, Antonin Jr. 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Metal‐Assembled Catenanes, Rotaxanes, and Knots
Authors: Armaroli Nicola , Chambron Jean‐Claude , Collin Jean‐Paul , Dietrich‐Buchecker Christiane , Flamigni Lucia , Kern Jean‐Marc , Sauvage Jean‐Pierre 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The Reactivity of Ground‐State Carbon Atoms with Unsaturated Hydrocarbons in Combustion Flames and in the Interstellar Medium
Authors: Kaiser Ralf I. , Mebel Alexander M. 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Application of Complex Macromolecular Architectures for Advanced Microelectronic Materials
Authors: Hedrick James L. , Magbitang Teddie , Connor Eric F. , Glauser Thierry , Volksen Willi , Hawker Craig J. , Lee Victor Y. , Miller Robert D. 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Intrinsic Deuterium Isotope Effects on NMR Chemical Shifts of Hydrogen Bonded Systems
Authors: Hansen Poul E. 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: NMR of Carbohydrates, Lipids and Membranes
Authors: Hounsell E. F. 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Crystal Structure of Rhodopsin: A G‐Protein‐Coupled Receptor
Authors: Stenkamp R. E. , Teller D. C. , Palczewski K. 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Active States of Rhodopsin
Authors: Ernst Oliver P. , Bartl Franz J. 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Using Pulsed Gradient Spin Echo NMR for Chemical Mixture Analysis. How To Obtain Optimum Results
Authors: Antalek Brian 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Gd3Pt4In12 and Tb3Pt4In12 — New Ternary Indides with Condensed Distorted [PtIn6] Trigonal Prisms.
Authors: Rodewald Ute Ch. , Zaremba Vasyl' I. , Galadzhun Yaroslav V. , Hoffmann Rolf‐Dieter , Poettgen Rainer 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
The Formation of ReS2 Inorganic Fullerene‐Like Structures Containing Re4 Parallelogram Units and Metal—Metal Bonds.
Authors: Coleman Karl S. , Sloan Jeremy , Hanson Neal A. , Brown Gareth , Clancy Gerald P. , Terrones Mauricio , Terrones Humberto , Green Malcolm L. H. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Hetero Diels—Alder Reactions of Nitroso Alkenes with Alkoxyallene Derivatives Bearing Carbohydrate Auxiliaries: Asymmetric Synthesis of 6H‐1,2‐Oxazines and Subsequent Reductive Transformations.
Authors: Zimmer Reinhold , Orschel Beate , Scherer Stefan , Reissig Hans‐Ulrich 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Remarkable Rate Acceleration of the Solvent‐Free Baeyer—Villiger Reaction on the Surface of NaHCO3 Crystals for Sterically Congested Cyclic and Acyclic Ketones.
Authors: Yakura Takayuki , Kitano Tomoko , Ikeda Masazumi , Uenishi Jun'ichi 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
1,2‐Adducts of 5‐Allyl‐2,3,5‐trichloro‐4,4‐dimethoxy‐2‐cyclopentenone with Ethyl Acetate Lithium Salt and Their Reactions with Iodine.
Authors: Vostrikov N. S. , Vasikov V. Z. , Spirikhin L. V. , Miftakhov M. S. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
anti Selective Dihydroxylation by the Ketimine Derivatives of the Allylic Amine in Monosubstituted Olefins.
Authors: Oh Joon Seok , Park Doh Yeon , Song Bu Sop , Bae Jae Gwang , Yoon Seung Woong , Kim Young Gyu 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Reaction of 1H,1H,ωH‐Perfluoroalkyl Sulfones with α‐Phenylglycine Methyl Ester.
Authors: Timoshenko V. M. , Nikolin Ya. V. , Lozinskii M. O. , Shermolovich Yu. G. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Highly Stereoselective Asymmetric 6π‐Azaelectrocyclization Utilizing the Novel 7‐Alkyl Substituted cis‐1‐Amino‐2‐indanols: Formal Synthesis of 20‐Epiuleine.
Authors: Tanaka Katsunori , Katsumura Shigeo 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Eclectic 1‐Aryl‐1,2‐diazabuta‐1,3‐dienes: Valuable Tools for the Preparation of Pyrrol‐2‐ones, 1‐Arylpyrazoles, 2‐(3‐Oxopyrazol‐4‐yl)malonates and 4‐(2‐Oxopyrrol‐3‐yl)pyrazol‐3‐ones.
Authors: Attanasi Orazio A. , De Crescentini Lucia , Favi Gianfranco , Filippone Paolino , Mantellini Fabio , Santeusanio Stefania 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Microwave‐Assisted Synthesis of 5‐Hydroxy‐5‐trichloromethyl‐4,5‐dihydroisoxazoles.
Authors: Martins Marcos A. P. , Beck Paulo , Cunico Wilson , Pereira Claudio M. P. , Sinhorin Adilson P. , Blanco Rogerio F. , Peres Rodrigo , Bonacorso Helio G. , Zanatta Nilo 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Free Radicals. Part 27. Synthesis of 2,6‐Diphenyl‐4‐[p‐(2,6‐diphenyl‐4‐pyridyl)phenyl]pyranyl.
Authors: Tanaseichuk B. S. , Pryanichnikova M. K. , Burtasov A. A. , Sysmanov V. V. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of N‐Methylmorpholinium 1‐Amino‐2,4‐dicyano‐4‐phenylcarbamoyl (ethoxycarbonyl)‐1,3‐butadiene‐1‐thiolates and Their Transformation into Pyridine‐2‐thiol Derivatives.
Authors: Dyachenko V. D. , Tkachev R. P. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis, Antibacterial and Analgesic Activity of 1‐(2‐Ethoxyethyl)‐4‐hydroxy(acyloxy)piperidine‐4‐carboxylic Acids.
Authors: Yu V. K. , Nagimova A. D. , Praliev K. D. , Shin S. N. , De Kimpe N. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthetic Transformations of Higher Terpenoids. Part 7. Synthesis of Tetrahydro‐β‐carbolines of the Labdane Series.
Authors: Chernov S. V. , Shul'ts E. E. , Shakirov M. M. , Tolstikov G. A. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Organic Reactions in Ionic Liquids: Ionic Liquid‐Accelerated Cyclocondensation of α‐Tosyloxyketones with 2‐Aminopyridine.
Authors: Xie Yuan‐Yuan , Chen Zhen‐Chu , Zheng Qin‐Guo 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of 6,7‐Dialkyl‐4‐amino‐3‐imino‐1,3‐dihydrofuro[3,4‐c]pyridines
Authors: Vasil'ev A. N. , Nikolaev A. N. , Lyshchikov A. N. , Nasakin O. E. , Kayukov Ya. S. , Tafeenko V. A. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Pyrido[2,3‐d]pyrimidines and Pyrimido[5′,4′:5,6]pyrido[2,3‐d]pyrimidines as New Antiviral Agents: Synthesis and Biological Activity.
Authors: Nasr Magda N. , Gineinah Magdy M. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis and Cytotoxic Activity of Esters and Nitriles of 1,3,5‐Triazinyldinitrobutyric Acids.
Authors: Gidaspov A. A. , Bakharev V. V. , Kachanovskaya E. V. , Yakunina N. G. , Bulychev Yu. N. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis and Antibacterial Activity of 3‐(5‐Methylisoxazol‐3‐yl)‐1,2,4‐triazolo[3,4‐B]‐1,3,4‐thiadiazine Derivatives.
Authors: Hui Xin‐Ping , Wang Qin , Zhang Qi , Zhang Zi‐Yi 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of Novel C2‐Aryl Pyrrolobenzodiazepines (PBDs) as Potential Antitumor Agents.
Authors: Cooper Nectaroula , Hagan David R. , Tiberghien Arnaud , Ademefun Temitope , Matthews Charles S. , Howard Philip W. , Thurston David E. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Studies on Nitrogen‐ and Sulfur‐Containing Heterocycles. Part 55. Synthesis, Chemical, Spectral and Biological Properties of 3,12‐Diaza‐6,9‐diazoniadispiro[5,2,5,3]heptadecane Derivatives.
Authors: Safonova T. S. , Shatukhina E. A. , Sedov A. L. , Turchin K. F. , Sokolova A. S. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Chelation‐Assisted Intramolecular Hydroacylation: Synthesis of Medium Ring Sulfur Heterocycles.
Authors: Bendorf Holly D. , Colella Christine M. , Dixon Elizabeth C. , Marchetti Melissa , Matukonis Alicia N. , Musselman Jeffrey D. , Tiley Tara A. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Efficient Highly Stereoselective Synthesis of Olefinic Macrocyclic Crown‐Formazans with the Z‐Configuration via Ring‐Closure Metathesis.
Authors: Ibrahim Yehia A. , Behbehani Haider , Ibrahim Maher R. , Abrar Nada M. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of Biologically Active 1‐Arylethylphosphonates.
Authors: Gulyukina N. S. , Dolgina T. M. , Bondarenko G. N. , Beletskaya I. P. , Bondarenko N. A. , Henry J.‐C. , Lavergne D. , Ratovelomanana‐Vidal V. , Genet J.‐P. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Tandem Michael‐Aldol Reaction via 6‐endo‐Dig Cyclization of Ynone‐Chalcogenides: Synthesis of 2‐Unsubstituted 3‐(Hydroxyalkyl)chalcogenochromen‐4‐ones.
Authors: Kataoka Tadashi , Kinoshita Hironori , Kinoshita Sayaka , Iwamura Tatsunori 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Merrillianin, a Unique seco‐Prezizaane‐Type Sesquiterpene, and (6R)‐Pseudomajucin from Illicium merrillianum.
Authors: Huang Jian‐Mei , Yang Chun‐Shu , Kondo Mamiko , Nakade Kosuke , Takahashi Hironobu , Takaoka Shigeru , Fukuyama Yoshiyasu 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis of 3‐O‐Acetylbetulinic and Betulonic Aldehydes by Swern Oxidation and Pharmacological Activity of Their Oximes.
Authors: Flekhter O. B. , Ashavina O. Yu. , Boreko E. I. , Karachurina L. T. , Pavlova N. I. , Kabal'nova N. N. , Savinova O. V. , Galin F. Z. , Nikolaeva S. N. , Zarudii F. S. , et al. et al. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
New Polyoxygenated Steroids Exhibiting Reversal of Multidrug Resistance from the Gorgonian Isis hippuris
Authors: Tanaka Junichi , Trianto Agus , Musman Musri , Issa Hamad H. , Ohtani Ikuko I. , Ichiba Toshio , Higa Tatsuo , Yoshida Wesley Y. , Scheuer Paul J. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Stereoselective Synthesis of β‐D‐Mannopyranosides with Reactive Mannopyranosyl Donors Possessing a Neighboring Electron‐Withdrawing Group.
Authors: Abdel‐Rahman Adel A.‐H. , Jonke Simon , El Ashry El Sayed H. , Schmidt Richard R. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Microwave‐Induced, Montmorillonite K10‐Catalyzed Ferrier Rearrangement of tri‐O‐Acetyl‐D‐galactal: Mild, Eco‐Friendly, Rapid Glycosidation with Allylic Rearrangement.
Authors: Shanmugasundaram Bhagavathy , Bose Ajay K. , Balasubramanian Kalpattu K. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis and Properties of Some Nitrogen‐Containing Derivatives of 1,3,5‐Triazine.
Authors: Mikhailichenko S. N. , Chesnyuk A. A. , Suslov V. I. , Shkrebets A. I. , Yukhonenko M. M. , Zaplishyi V. N. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis and Complexing Ability of a C‐Pivot Type of Double‐Armed 15‐Crown‐5 Ethers Toward Alkali Metal Cations.
Authors: Nakatsuji Yohji , Muraoka Masahiro , Kajiya Hiroyuki , Zhang Wanbin , Kida Toshiyuki , Ikeda Isao 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Nucleophilic Borylation of Benzyl Halides with Bis(pinacolato)diboron Catalyzed by Palladium(0) Complexes.
Authors: Ishiyama Tatsuo , Oohashi Zengo , Ahiko Taka‐aki , Miyaura Norio 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Stereoselectivity in the Fe(II)/Cu(II)‐Mediated Homolytic Decomposition of the cis‐ and trans‐Isomers of Pinane Hydroperoxide.
Authors: Lempers H. E. B. , Sheldon R. A. , Swift K. A. D. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
3‐Pyrroline Containing Arylacetamides: A Novel Series of Remarkably Selective ϰ‐Agonists.
Authors: Mou Qi‐Yong , Chen Jie , Zhu You‐Cheng , Zhou De‐He , Chi Zhi‐Qiang , Long Ya‐Qiu 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Use of Phenyl 2‐α‐Selenoglycosides of N‐Acetylneuraminic Acid as a Glycosyl Donor for the Glycosylation Reactions.
Authors: Ikeda Kiyoshi , Sugiyama Yuji , Tanaka Kiyoshi , Sato Masayuki 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Novel 20‐Carbonate Linked Prodrugs of Camptothecin and 9‐Aminocamptothecin Designed for Activation by Tumor‐Associated Plasmin
Authors: de Groot Franciscus M. H. , Busscher Guuske F. , Aben Rene W. M. , Scheeren Hans W. 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Synthesis and Biological Evaluation of Novel D‐2′‐Azido‐2′,3′‐dideoxyarabinofuranosyl‐4′‐thiopyrimidines and Purines.
Authors: Kim Hea Ok , Park Yong Hee , Moon Hyung Ryong , Jeong Lak Shin 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Preparation of Chiral 4‐Benzyloxymethyldihydrofuran‐2‐one Using Lipase‐Catalyzed Kinetic Resolution: Synthesis of (‐)‐Virginiae Butanolide C (VB C).
Authors: Takabe Kunihiko , Mase Nobuyuki , Matsumura Hidetoshi , Hasegawa Takehiro , Iida Yasuhiro , Kuribayashi Hisashi , Adachi Kenji , Yoda Hidemi , Ao Masato 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
1‐(Bicyclopiperazinyl)ethylindoles and 1‐(Homopiperazinyl)ethylindoles as Highly Selective and Potent 5‐HT7 Receptor Ligands.
Authors: Issac Methvin B. , Xin Tao , O'Brien Anne , St‐Martin David , Naismith Angela , MacLean Neil , Wilson Julie , Demchyshyn Lidia , Tehim Ashok , Slassi Abdelmalik 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Catalysis Using Supercritical or Subcritical Inert Gases under Split‐Phase Conditions
Authors: Jessop Philip G. , Eckert Charles A. , Liotta Charles L. , Bonilla R. Jason , Brown James S. , Brown Richard A. , Pollet Pamela , Thomas Colin A. , Wheeler Christy , Wynne Dolores 
Published: May 2010

AbstractFor Abstract see ChemInform Abstract in Full Text.
Adjusting transgene expression levels in lymphocytes with a set of inducible promoters
Authors: Danke Christina , Grünz Xandra , Wittmann Jürgen , Schmidt Andreas , Agha‐Mohammadi Siamak , Kutsch Olaf , Jäck Hans‐Martin , Hillen Wolfgang , Berens Christian 
Published: May 2010

AbstractBackgroundInducible gene expression systems are powerful research tools and could be of clinical value in the future, with lymphocytes being likely prime application targets. However, currently available regulatable promoters exhibit variation in their efficiency in a cell line‐dependent‐manner and are notorious for basal leakiness or poor inducibility. Data concerning the regulatory properties of different inducible promoters are scarce for lymphocytes. In the present study, we report a comprehensive analysis of how various inducible promoters perform and how their combination with a transsilencer and a reverse transactivator can result in optimally controlled gene expression in T‐cells.MethodsThe performance of the tetracycline‐regulated (Tet)‐inducible promoters Tet‐responsive element (TRE), mouse mammary tumor virus (MMTV)/TRE, TREtight and second generation TRE (SG/TRE) was compared in several B‐cell lines and in Jurkat T‐cells using transient transfections in combination with Tet‐On. To monitor transgene expression in a Jurkat cell line containing a transsilencer and a reverse transactivator, expression cassettes encoding enhanced green fluorescent protein, CD123 or a constitutively active, cytotoxic caspase‐3 were flanked with insulators and stably integrated. The performance of TREtight and SG/TRE was furthermore analysed in transiently transfected primary CD4+ human T‐cells.ResultsThe promoters exhibit greatly diverging characteristics. MMTV/TRE permits moderate, TRE and TREtight permits intermediate and SG/TRE permits very high expression levels. TRE and SG/TRE are leaky, whereas MMTV/TRE and TREtight provide stringent expression control. Tetracycline derivatives add flexibility to transgene expression by introducing intermediate expression levels.ConclusionsThe different expression profiles of the promoters increase the flexibility to adjust transgene expression levels. The promoters provide an additional option to optimize system performance for many applications. Copyright © 2010 John Wiley & Sons, Ltd.