bluuebunny/crossref_metadata_2025_split huggingface dataset:
434-P: Type 2 Diabetes Rather than the Presence of Significant Coronary Stenoses Is Associated with Quality of Life in Angiographied Coronary Patients
Authors: RATZ MAGDALENA , VOGEL JOHANNES , ELSNER PASCAL , PLATTNER THOMAS , VONBANK ALEXANDER , MADER ARTHUR , LARCHER BARBARA , LEIHERER ANDREAS , MUENDLEIN AXEL , FRICK MATTHIAS , DREXEL HEINZ , SAELY CHRISTOPH H. 
Published: June 2024

Chronic medical conditions such as type 2 diabetes (T2DM) or coronary artery disease (CAD) and their treatment have a crucial impact on patients’ daily life. The EQ-5D-5L questionnaire is a validated and widely used tool to measure the health-related quality of life (HRQL). We investigated and compared the HRQL in patients with or without T2DM and CAD. We included 481 consecutive patients undergoing coronary angiography for the evaluation of established or suspected stable CAD in a tertiary care setting in Central Europe. Patients’ HRQL was measured using the EQ-5D-5L, comprising the EQ index calculated from the domains: mobility, self-care, usual activities, pain/discomfort, and anxiety/depression. A response rate of 88.1% was achieved. From our patients, 166 (34.5%) had T2DM according to ADA criteria, and 254 (52.8%) had significant CAD with stenoses ≥50% at angiography. Patients with T2DM showed significantly lower EQ index values than patients without T2DM (0.891 [IQR=0.783-0.970]) vs. 0.943, [IQR=0.861-1.0], p=0.002)). Regarding the different dimensions of the questionnaire, patients with T2DM reported to have significantly more problems with anxiety and depression (p=0.002) and mobility (p&lt;0.001) than nondiabetic patients. EQ index values in contrast did not differ significantly between patients with vs. those without significant CAD. Accordingly, in analysis of covariance T2DM (F=7.38, p=0.007) but not significant CAD (F=1.74, p=0.183) predicted EQ index values after adjustment for age, sex and body-mass-index. We conclude that T2DM rather than the presence of significant CAD is associated with quality of life in angiographied coronary patients.

Disclosure
M. Ratz: None. J. Vogel: None. P. Elsner: None. T. Plattner: None. A. Vonbank: None. A. Mader: None. B. Larcher: None. A. Leiherer: None. A. Muendlein: None. M. Frick: None. H. Drexel: None. C.H. Saely: None.
459-P: Association of Intraoperative Mean Glucose with Clinical Outcomes in Patients with Acute Ischemic Stroke Treated by Mechanical Thrombectomy
Authors: WENG JIAHAO , ZHENG XUEYING , LUO SIHUI , ZHOU YONGWEN , ZHANG HONGQIANG , WANG XULIN , SHI JIE , WENG JIANPING , WANG CHAOFAN , ZHANG FENG , ZHANG PAN , XIA YUE , SUN WEN 
Published: June 2024

Introduction & Objective: Poststroke hyperglycemia is common and associated with adverse outcomes for patients with acute ischemic stroke (AIS). However, the evidence of glucose level during mechanical thrombectomy (MT) are limited. This study aimed to investigate the association between intraoperative glucose level based on continuous glucose monitoring (CGM) system and clinical outcomes.
Method: In this single-center, retrospective and observational study, we consecutively included AIS patients treated by MT within 24 hours from symptom onset. All subjects received a CGM system iPro2® when they arrived at the interventional room. Clinical outcomes included in-hospital mortality, in-hospital neurological improvement and deterioration, 3-month function independence and mortality. Logistic regression estimated the association between intraoperative mean glucose and outcomes.
Results: A total of 123 AIS patients [women: 50 (40.65%), median age: 71 (60-78), diabetes: 25 (20.33%)] were included. During MT therapy, the median period of glucose monitoring was 1.04 hours, and the median mean glucose level was 6.87 mmol/L. Mean glucose was independently related to in-hospital mortality (OR 1.539, 95% CI 1.078-2.315, p = 0.026), 3-month mortality (OR 1.418, 95% CI 1.019-2.048, p = 0.048) and functional independence (OR 0.695, 95% CI 0.493-0.931, p = 0.023) after adjusting for potential confounders. There was no association of intraoperative mean glucose with in-hospital neurological improvement (OR 0.876, 95% CI 0.695-1.090, p = 0.242) and neurological deterioration (OR 1.159, 95% CI 0.909-1.499, p = 0.244).
Conclusion: Among AIS patients who received MT treatment, intraoperative mean glucose was associated with in-hospital mortality, 3-month adverse neurological function and mortality.

Disclosure
J. Weng: None. X. Zheng: None. S. Luo: None. Y. Zhou: None. H. Zhang: None. X. Wang: None. J. Shi: None. J. Weng: None. C. Wang: None. F. Zhang: None. P. Zhang: None. Y. Xia: None. W. Sun: None.

Funding
Clinical Medical Research Transformation Project of Anhui Province (Grant No. 202204295107020026).
46-OR: Palmitic Acid Coordinates Impaired Adipose Tissue ICOShigh Treg Immunosuppression and Systemic Metabolic Disturbance during Obesity
Authors: SU WEITONG , CUI AOYUAN , HUANG MENGYAO , LIN JING , DING DONG , ZHENG ZENGPENG , JIANG YANG , CAI GENXIANG , LI WENJING , WEI SHUANG , SHEN JIAXIN , FANG XIA , WEN JIAN , HUO YANLING , WEI XIAOYI , LI LIANJIA , LIU ZHAN , QIAN KEYU , ZHAO JIUXIANG , LI YU 
Published: June 2024

Treg cells serve essential roles in immunological tolerance and tissue homeostasis remodeling. However, the precise mechanism by which Treg cells specifically regulate the immune response in adipose tissue during obesity remains largely unexplored. Here we show that Foxp3, the master transcription factor of Treg cells, is negatively regulated by CREBZF, which mediates the immunosuppression of Treg cells in visceral adipose Treg cells. We found that CREBZF was dramatically induced in Treg cells from visceral adipose tissue (VAT) of obese subjects and epididymal adipose tissue (eWAT) of mice. Interestingly, palmitic acid (PA), but not insulin or lactate, promotes the expression of CREBZF in Treg cells. Treg cell-specific CREBZF deficiency significantly attenuated high-fat high sucrose (HFHS) diet-induced obesity, hyperglycemia and insulin resistance. Moreover, CREBZF deletion exhibited ameliorated inflammation and hypertrophic morphology in eWAT and enhanced systemic energy expenditure. Furthermore, obesity induced Treg cells loss, impaired Foxp3 expression and IL-10 production were reversed by CREBZF deletion in Treg cells from eWAT of mice, suggesting a key role of CREBZF in regulating the stability and immunosuppression of Treg cells. Mechanistically, single-cell transcriptomic analysis has identified a ICOShigh Treg cell subset, which is distinct from previously characterized adipose Treg cell populations and exhibited enhanced immunosuppressive function and more accumulation in CREBZF-deficient mice. Importantly, expression levels of CREBZF are negatively correlated with Foxp3 in VAT of obese human and eWAT of mice. Collectively, these studies uncover a specific adipose ICOShigh Treg subpopulation, which plays crucial roles in palmitic acid-induced immunosuppression restriction and systemic metabolic disorders.

Disclosure
W. Su: None. A. Cui: None. M. Huang: None. J. Lin: None. D. Ding: None. Z. Zheng: None. Y. Jiang: None. G. Cai: None. W. Li: None. S. Wei: None. J. Shen: None. X. Fang: None. J. Wen: None. Y. Huo: None. X. Wei: None. L. Li: None. Z. Liu: None. K. Qian: None. J. Zhao: None. Y. Li: None.

Funding
National Key R&D Program of China (2019YFA0802502), National Natural Science Foundation of China (81925008), Shanghai Science and Technology Commission (19140903300)
461-P: Metabolic Profile Alterations in Patients with Obstructive Sleep Apnea and Type 2 Diabetes
Authors: TUNA KUBRA , CHAGANI FATIMA , DANYLUK ALEXANDRIA , KESSLER ALEEZA T.F. , HADIGAL SUSHEELA , LEEY JULIO A. 
Published: June 2024

The co-occurrence of Obstructive Sleep Apnea (OSA) in individuals with Type 2 Diabetes (T2D) is well-established. Several studies have shown that T2D patients with moderate to severe OSA exhibit a more unfavorable glycemic profile. In this retrospective analysis, we conducted a chart review of 147 patients with T2D who had a sleep study. Within this cohort, 115 patients (78%) were categorized within the OSA group based on sleep study results, while 32 (28%) were classified in the no-OSA group. Both groups were similar in age, diabetes duration, and the presence of micro and macrovascular complications. Hypertension, higher BMI, and increased neck circumferences was more prevalent in the OSA group. Hemoglobin A1c (HbA1c) levels were similar prior to the initiation of sleep therapy (7.6% vs. 7.7%, P=0.8), patients who commenced positive airway pressure (PAP) therapy exhibited notable improvements in HbA1c levels, with values of 7.1% at one month (P=0.06) and 7.0% at three months post-therapy initiation from 7.6% (P=0.01). Importantly, the OSA and no-OSA groups demonstrated similar levels of serum glucose, lipid profiles, and FIB-4 scores. In summary, our findings suggest that individuals with coexisting OSA and T2D exhibit a constellation of unfavorable metabolic indicators, including increased body weight (246.5±60.2 vs 201.5±50.1 lbs, P = 0.00006 p&lt;0.01), increased complexity of diabetes, and increased incidence of fatty liver disease. The application of PAP therapy appears promising in terms of enhancing glycemic control, even in the short term. Given the marked prevalence of concurrent OSA and T2D, it is imperative to establish more effective treatment strategies for these individuals, who are predisposed to a higher risk of metabolic and cardiovascular diseases.

Disclosure
K. Tuna: None. F. Chagani: None. A. Danyluk: None. A.T.F. Kessler: None. S. Hadigal: None. J.A. Leey: None.

Funding
National Institutes of Health (UL1TR001427)
475-P: Peroxisome Proliferator-Activated Receptor Delta (PPARδ)—A Key Modulator in the Pathogenesis of Diabetes Mellitus and Mycobacterium Tuberculosis Comorbidity
Authors: ALSAEED HALEMAH , ALMULLA FAHD , AHMAD RASHEED , ALRASHED FATEMA 
Published: June 2024

The interplay between lipid metabolism and immune response in macrophages plays a pivotal role in various infectious diseases, notably tuberculosis (TB). Herein, we illuminate the modulatory effect of heat-killed Mycobacteria tuberculosis (HKMT) on macrophage lipid metabolism and its implications on the inflammatory cascade. Our findings demonstrate that HKMT potently activates the lipid scavenger receptor, CD36, instigating lipid accumulation. While CD36 inhibition mitigated lipid increase, it unexpectedly exacerbated the inflammatory response, marked by elevated CCL2 secretion. Intriguingly, this paradoxical effect was linked to an upregulation of PPARδ, distinct from its PPAR counterparts. Functional analyses employing PPARδ modulation revealed its central role in regulating both lipid dynamics and inflammation, suggesting it as a potential therapeutic target. Moreover, peripheral blood mononuclear cells (PBMCs) from diabetic individuals, a demographic at amplified risk of TB, exhibited heightened PPARδ expression and inflammation, further underscoring its pathological relevance. Targeting PPARδ in these cells effectively dampened the inflammatory response, offering a promising therapeutic avenue against TB, especially for those with diabetes.

Disclosure
H. AlSaeed: None. F. Almulla: None. R. Ahmad: None. F. Alrashed: None.

Funding
Kuwait Foundation for the Advancement of Sciences (RA CB-2023-017)
491-P: Transplantation of Dental Pulp Stem Cells Ameliorates Diabetic Muscle Atrophy and Inflammation and Improves Diabetic Peripheral Neuropathy
Authors: NARUSE KEIKO , OMI MAIKO , HATA MASAKI , NAKAMURA NOBUHISA , MIYABE MEGUMI , SASAJIMA SACHIKO , OZAKI REINA , OZAWA SHOGO , TAKEBE JUN , MATSUBARA TATSUAKI 
Published: June 2024

Objectives: Diabetic peripheral neuropathy is associated with neurogenic muscle atrophy. The development of successful therapies for nerve damage and muscle atrophy must be needed for patients with DPN. In this study, we investigated the effects of dental pulp stem cell (DPSC) transplantation on skeletal muscles in streptozotocin (STZ)-induced diabetic rats.
Methods: DPSCs were isolated and expanded from dental pulp of 6-wk old Sprague-Dawley rats. Cultured DPSCs were transplanted into the unilateral hindlimb skeletal muscles of normal and streptozotocin-induced diabetic rats. Four weeks after DPSC transplantation, the impacts of DPSC transplantation on hindlimb skeletal muscles and peripheral nerves were examined.
Results: Diabetic rats showed significant decreased the muscle fiber cross-sectional area and increased the CD68-positive inflammatory cell infiltration in gastrocnemius muscles, which were ameliorated by DPSC transplantation. Gene expression analyses revealed that DPSC transplantation increased PGC1α and suppressed atrogin-1 and TNF-α in diabetic skeletal muscles. DPSC transplantation significantly ameliorated decreased-FGF2 gene expression with the improvement of motor and sensory nerve conduction velocities in diabetic rats.
Conclusions: We identiﬁed that DPSC transplantation into hindlimb skeletal muscle could ameliorate diabetic muscle atrophy and inflammation and improve diabetic peripheral neuropathy.

Disclosure
K. Naruse: None. M. Omi: None. M. Hata: None. N. Nakamura: None. M. Miyabe: None. S. Sasajima: None. R. Ozaki: None. S. Ozawa: None. J. Takebe: None. T. Matsubara: None.
50-OR: Metabolic Dysfunction Attenuates D-dopachrome Tautomerase (DDT) Expression in the Heart—A Key Mechanism Exacerbates Myocardial Post-ischemic Injury
Authors: LI LISHA , QI YADAN , CUI NI , LENG LIN , WU HONG , BUCALA RICHARD , QI DAKE 
Published: June 2024

Metabolic abnormalities affect clinical recovery and long-term survival in patients with acute myocardial infarction, but the underlying mechanisms remain unclear. D-dopachrome tautomerase (DDT) in cardiomyocytes protects the heart from injury during hypoxia-ischemia in mice. Our present study found that metabolic dysfunction induced by high fat diet (HFD) was associated with reduced cardiac DDT expression and aggravated cardiac injury following ischemia-reperfusion. Supplementation of DDT prior to ischemia decreased post-ischemic injury in these hearts, suggesting that DDT reduction is an important mechanism regulating post-ischemic cardiac injury associated with metabolic dysfunction. Among all the major fatty acid species in HFD, high palmitic acid (PA) triggered the expression and activation of protease activated receptor 2 (PAR2), which upregulated the transcriptional factors, CREB1 and FOXO1 leading to downregulation of DDT expression in the heart. PAR2 stimulated ERK phosphorylation, thereby upregulating CREB1 phosphorylation. Attenuation of CREB by siRNA significantly decreased FOXO1 expression and accumulation in the nucleus, thereby reducing cardiac DDT expression. Accordingly, PAR2 deficient mice exhibited normal ERK and CREB phosphorylation and DDT levels in the heart following HFD and reversed cardiac function recovery following reperfusion. Overall, our data reveal for the first time a novel role for DDT in mediating myocardial ischemia-reperfusion injury associated with metabolic dysfunction.

Disclosure
L. Li: None. Y. Qi: None. N. Cui: None. L. Leng: None. H. Wu: None. R. Bucala: None. D. Qi: None.

Funding
This study was supported by National Sciences and Engineering Research Council of Canada (NSERC: RGPIN-2017-04542) and Canadian Institutes of Health Research (CIHR Project Grant: PJT-156116).
504-P: Prevalence and Risk Factors of Diabetic Retinopathy in South Korea’s Population
Authors: LEE MIN-KYUNG , HAN KYUNGDO , KWON HYUK-SANG 
Published: June 2024

Introduction & Objective: Diabetic retinopathy is a long-term microvascular complication of diabetes, and its prevalence has increased over recent years worldwide. The purpose of this study is to provide national representative statistics on the epidemiology and risk factors of diabetic retinopathy in Korean diabetic patients.
Methods: We conducted a cross-sectional study using the Korea National Health and Nutrition Examination Survey between 2017 and 2020. Participants aged ≥40 years who diagnosed with diabetes mellitus were analyzed. The prevalence of diabetic retinopathy during the entire period was estimated using the weighted percentage as mean and standard error. We also assessed adjusted risk factors for diabetic retinopathy using the logistic regression models.
Results: Among a total of 2,221 subjects (mean age, 60.8 years; 56.85% male subjects), 451 subjects had diabetic retinopathy, and the overall weighted prevalence of diabetic retinopathy was 20.64±1.06%. The prevalence of diabetic retinopathy tended to increase with higher HbA1c levels and longer duration of diabetes (P for trend &lt;0.001). The adjusted odd ratios for incident diabetic retinopathy were 1.348 (95% CI 0.893-2.037), 2.012 (95% 1.385-2.922), 2.799 (95% CI 1.777-4.408), 3.406 (95% CI 1.971-5.886), and 5.85 (95% CI 3.273-10.457) for HbA1c levels of 6.5-6.9%, 7.0-7.9%, 8.0-8.9%, 9.0%-9.9% and ≥10.0%, respectively. And they were 1.499 (95% CI 0.992-2.267), 2.066 (95% 1.385-3.082), 20.396 (95% CI 1.542-3.724), and 3.622 (95% CI 2.386-5.498) for non-awareness, diabetes duration of &lt;5 years, 5-10 years, 10-15 years, and ≥10 years, respectively.
Conclusion: The prevalence of diabetic retinopathy was estimated to increase in patients with poor control and long duration of diabetes. Hyperglycemia and diabetes duration are determinants for diabetic retinopathy in Korea's population.

Disclosure
M. Lee: None. K. Han: None. H. Kwon: None.

Funding
The National Research Foundation of Korea (NRF) grant funded by the Korea government (MSIT) (2021R1A2C2013890 and 2022R1F1A061069)
511-P: Serum Tsukushi Is Associated with HDL Dysfunction in Type 2 Diabetes and Nonalcoholic Fatty Liver Disease
Authors: TAN KATHRYN C.B. , LAM SUM , LUI DAVID T. , YI FONG HO , WONG YING , SHIU SAMMY , HO LEE CHI 
Published: June 2024

Introduction & Objective: Tsukushi (TSK) is a recently identified hepatokine and animal studies have suggested that TSK can affect systemic cholesterol homeostasis. Gain- and loss-of-function experiments in mice showed that TSK reduced circulating HDL-cholesterol (HDL-C). We therefore investigated whether TSK was associated with HDL-C and HDL function in type 2 diabetes with co-existing non-alcoholic fatty liver disease (NAFLD).
Methods: Vibration‑controlled transient elastography was performed in 565 subjects with type 2 diabetes. The degree of hepatic steatosis and fibrosis was reflected by controlled attenuation parameter (CAP) and liver stiffness (LS) respectively, and the presence of NAFLD was defined as CAP ≥248 dB/m. Serum TSK was measured by ELISA. Cholesterol efflux capacity (CEC), an indicator of HDL function, was determined by measuring the efflux of [3H]-cholesterol from RAW264.7 macrophages to apolipoprotein B-depleted serum.
Results: NAFLD was present in 430 subjects (76%). The age, proportion of male subjects and duration of diabetes were comparable between those with and without NAFLD. However, individuals with NAFLD had higher BMI (p&lt;0.01) and serum TSK levels [97.0 ng/ml (63.8 - 136.3) versus 81.5 ng/ml (57.8 - 119.4) respectively, p&lt;0.01], lower HDL-C (p&lt;0.01) and CEC (18.0 ± 4.9% versus 19.1 ± 5.9% respectively, p&lt;0.05) than those without NAFLD. Serum TSK correlated with HDL-C (r = -0.19, p&lt;0.01) and with CEC (r = -0.16, p&lt;0.01). Linear regression analysis showed that TSK, HDL-C and the use of thiazolidinediones were significant and independent determinants of CEC, in a model also consisting of age, sex, smoking, BMI, and the use of various anti-diabetic agents and lipid lowering therapy.
Conclusion: elevated TSK level was associated with reduced HDL-C, and HDL dysfunction with impaired CEC in type 2 diabetes and co-existing NAFLD.

Disclosure
K.C.B. Tan: None. S. Lam: None. D.T. Lui: None. H. Fong: None. Y. Wong: None. S. Shiu: None. C. Lee: None.
512-P: The Effect of Remnant Cholesterol on the Onset of Diabetes Mellitus
Authors: KANAMOTO AYUMI , MIYAKE TERUKI , FURUKAWA SHINYA , MATSUURA BUNZO , YOSHIDA OSAMU , MIYAZAKI MASUMI , SHIOMI AKIHITO , NAKAGUCHI HIRONOBU , HIROOKA MASASHI , ABE MASANORI , HIASA YOICHI 
Published: June 2024

Remnant cholesterol (Rem-C) is the cholesterol content found in triglyceride-rich lipoproteins, which are produced in the metabolic process of VLDL and chylomicrons. REM-C is associated with an increased risk of cardiovascular disease and is a target of lipid-lowering therapies. Conversely, insulin resistance is linked to increased levels of Rem-C. However, the effect of Rem-C levels on the onset of diabetes mellitus (DM) has not been adequately clarified. Therefore, this study aims to examine the association between Rem-C levels and the onset of diabetes. We reviewed the records of 13,044 nondiabetic individuals (6,499 males, 6,545 females) who underwent health check-ups at least twice, with a median age of 44 (IQR 37-51) years. The observation period spanned 5.86 (0.60-16.92) years. DM onset was determined based on HbA1c≧6.5% or fasting plasma glucose≧126mg/dL. Four hundred sixty subjects developed DM. In univariate analysis, an increase in Rem-C significantly raised the risk of DM onset (HR: 1.017, 95% CI: 1.014-1.019). Similar results were obtained in multivariate analysis (adjusted HR: 1.009, 95% CI: 1.005-1.013), adjusting for gender, age, body mass index, systolic blood pressure, fasting blood glucose, alanine aminotransferase, creatinine, family history of diabetes, and smoking history.
In conclusion, a high level of Rem-C is a risk factor for the onset of DM. Therefore, Rem-C may serve as a useful surrogate marker for DM onset, and interventions, such as lifestyle guidance and drug therapy, focusing on Rem-C may help suppress the onset of DM.

Disclosure
A. Kanamoto: None. T. Miyake: None. S. Furukawa: Speaker's Bureau; Lilly Diabetes, Novo Nordisk, Mitsubishi Tanabe Pharma Corporation. B. Matsuura: None. O. Yoshida: None. M. Miyazaki: None. A. Shiomi: None. H. Nakaguchi: None. M. Hirooka: None. M. Abe: None. Y. Hiasa: None.
521-P: Association between the Hepatic Steatosis and the TyG Index in a Population of Healthy Adults in Korea
Authors: BAE LEE SANG 
Published: June 2024

It has been established that the triglyceride glucose (TyG) index is a reliable indicator of insulin resistance (IR), which strongly impact to development of hepatic steatosis and non-alcoholic fatty liver disease (NAFLD). However, the relation between NAFLD and the TyG index remains not fully understood. We examined and analyzed the association between NAFLD and the TyG index in a population of healthy adults in Korea.
A total of 5158 participants who visit health promotion center, underwent blood sampling and abdominal ultrasonography were analyzed in this study. Degree of hepatic steatosis and NAFLD was classified via ultrasonograpic findings.
According to changes in the TyG index, significant changes in metabolic parameters were observed. The prevalence of NAFLD also increased proportionally with the increase in the TyG index. We also performed additional exploration using HOMA-IR with logistic regression analysis. The TyG index showed more strong predictive power for prevalence of NAFLD than HOMA-IR. Comparing the lowest and highest quartiles of the TyG index and HOMA-IR, the odds ratio for NAFLD prevalence in the TyG index was 3.11 (95% CI: 2.46-3.93; P for trend &lt;0.01), which is higher than the value of 1.82 in HOMA-IR (95% CI: 1.35-2.45; P for trend &lt;0.01).
As a result of our study, prevalence of NAFLD showed strong association with the TyG index. Additionally, the TyG Index showed stronger predictive value for NAFLD compared to HOMA-IR.

Disclosure
S. Lee: None.