bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immobilization of magnetic magnetite nanoparticle film on polyamide fabric
Authors: Zhang H. , Zheng J. Y. 
Published: February 2012

AbstractPolyamide (PA) fabric loaded with magnetite (Fe3O4) nanoparticle film was prepared by a simple chemical coprecipitation method. The surface morphology, chemical and phase constituents, crystalline structure, magnetic behavior, thermal stability, and mechanical properties of the Fe3O4‐coated fabric were determined by means of scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, X‐ray diffraction, vibrating sample magnetometry, thermogravimetric analysis, differential scanning calorimetry, Fourier transform infrared spectroscopy, and tensile testing. The results show that a thin membrane of Fe3O4 nanoparticles was immobilized on the surface of THE PA fabric. The saturation magnetization of the Fe3O4‐coated fabric was 27.5 emu/g. The onset decomposition temperature increased slightly, but the initial endothermic temperature decreased. The Fe3O4 nanoparticles were deposited on the fiber surface by mechanical anchoring action. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Effect of the manufacturing conditions on the structure and performance of thin‐film composite membranes
Authors: Zhang Rui‐Xin , Vanneste Johan , Poelmans Lore , Sotto Arcadio , Wang Xiao‐Lin , Van der Bruggen Bart 
Published: February 2012

AbstractA systematic investigation of the influence of the manufacturing conditions on the structure and performance of thin‐film composite (TFC) membranes is presented for polyamide (PA) supported by poly(ether sulfone) (PES). The TFC membranes were composed of an ultrathin PA layer synthesized by interfacial polymerization on top of a porous PES support layer formed by immersion precipitation. For the PES support layer, the role of the wetting pretreatment, initial casting film thickness, and relative air humidity were studied. Assuming a strong correlation between the thermodynamics and the hydrodynamics of the casting process, we derived new insights from scanning electron microscopy images and the experimental data. In view of optimization of the flux through the membranes, a wetting pretreatment should be avoided. Important polymer savings were obtained without a loss of performance through a decrease in the casting thickness in combination with the use of a very smooth support. Last but not least, a high air humidity during casting was found to inhibit the formation of a dense, defect‐free skin layer. For the PA layer, the interfacial polymerization method, the drying method, and the curing time were studied. The clamping of the membrane in a frame with one side in contact with the piperazine (PIP) solution and the other side to the air yielded the highest membrane flux and rejection with the lowest use of PIP and trimesoylchloride solution. Because of the absence of a uniform PIP solution layer for some drying methods, nodular PA structures could be observed in the macrovoids of the underlying PES layer because of hexane intrusion; this resulted in a dramatic decrease in the flux. Moreover, the omission of the drying step did not result in a significant loss of performance and enhanced the ease of operation. Finally, a curing time of 8 min was found to be optimal. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Processing and tribology of thermoplastic polyurethane particulate composite materials
Authors: Golaz B. , Tetouani S. , Diomidis N. , Michaud V. , Mischler S. 
Published: February 2012

AbstractThe processing, mechanical and tribological properties of wax containing thermoplastic polyurethane–filler composites were studied for different weight ratios of graphite, TiO2, MoS2, and ZrO2 microparticles and SiO2 nanoparticles. The composites were compounded by extrusion and processed by compression molding. The rheological, thermal, and mechanical properties were measured, and the wear characteristics were tested with ball‐on‐plate reciprocating tribometer tests under fixed friction conditions and then observed by scanning electron microscopy. Correlations between the friction, wear, and mechanical properties were observed, and their mechanisms are discussed. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Long‐term changes in unionid assemblages in the Rio Grande, one of the World's top 10 rivers at risk
Authors: Karatayev Alexander Y. , Miller Thomas D. , Burlakova Lyubov E. 
Published: February 2012

ABSTRACT

According to the World Wildlife Fund, the Rio Grande is the most endangered river system in the North American continent and one of the World's top 10 rivers at risk, but is globally important for freshwater biodiversity. Unionid bivalves of the Rio Grande river basin used to be represented by a unique assemblage, including four endemic species (Truncilla cognata, Potamilis metnecktayi, Popenaias popeii, and Quadrula couchiana); however, surveys from 1998–2001 failed to recover any live endemic unionid species suggesting a sharp decrease in their populations and potential of extinction.
Intensive surveys (162 sites sampled) conducted by the authors from 2001–2011 on the Rio Grande and its tributaries in Texas recovered live T. cognata, P. metnecktayi, and the largest population of P. popeii ever reported. Overall the unionid assemblage of the Rio Grande basin has changed considerably during the last century.
Decline in species diversity, range fragmentation, local extirpations, and introduction of widespread common species were documented. Two species (Q. couchiana and Quincuncina mitchelli) are locally extinct. Potamilus metnecktayi and T. cognata have been extirpated from the Pecos River and their ranges in the Rio Grande have been reduced. Popenaias popeii has been extirpated from the Pecos River and Las Moras Creek along with the reduction and fragmentation of its range in the Devils River and Rio Grande.
Among the environmental factors responsible for the degradation of unionid assemblages in the Rio Grande river basin, the most important are impoundments, habitat degradation, salinization, pollution, and over‐extraction of water. Copyright © 2012 John Wiley & Sons, Ltd.
Development of dipstick‐based immuno‐chemiluminescence techniques for the rapid detection of dichlorodiphenyltrichloroethane
Authors: Baker Syed , Vinayaka Aaydha C. , Manonmani Haravey K. , Thakur Munna S. 
Published: February 2012

ABSTRACTThe occurrence of organochlorine pesticides in the environment has been a major concern, due to their high persistence and the possible impacts of their exposure to humans. Dichlorodiphenyltrichloroethane (DDT) is most hazardous and one of the most widely used organochlorine insecticides. DDT and its main metabolites are highly stable to physical, chemical and biological degradation and are therefore still being detected in many parts of the world. The present study describes dipstick‐based immuno‐chemiluminescence method for the detection of DDT with high sensitivity. Anti‐DDT antibodies raised in chicken (IgY) were used as the biological sensing elements by immobilizing onto nitrocellulose membrane strips in a chemiluminescence (CL)‐based dipstick technique. The photons generated during the biochemical interaction were directly proportional to the DDT concentration. A mean recovery of 81.2–95.6% was obtained for DDT‐spiked fruit juice samples with 2.8–4.6% relative standard deviation (RSD). Using the proposed dipstick‐based immuno‐CL method, DDT was detected with linearity in the range 0.05–1 ng/mL, having a limit of detection (LOD) of 0.05 ng/mL. This method can be used for the rapid, reliable detection of DDT pesticide. Copyright © 2012 John Wiley & Sons, Ltd.
A novel reddish‐orange phosphor, NaLi2PO4:Eu3+
Authors: Shinde K. N. , Dhoble S. J. 
Published: February 2012

ABSTRACTA series of Eu3+‐activated NaLi2PO4 novel phosphors was synthesized by the solid‐state reaction method. The X‐ray diffraction (XRD) and photoluminescence (PL) properties of these phosphors were investigated at room temperature. The excitation spectra indicate that these phosphors can be effectively excited by near‐UV (370–410 nm) light. The emission spectra exhibit strong reddish‐orange performance, which is due to the 5D0 → 7FJ transitions of Eu3+ ions. The orange emission from transition 5D0 → 7F1 is dominant over that of 5D0 → 7F2. The concentration quenching of Eu3+ was observed in NaLi2PO4:Eu3+ when the Eu concentration was at 1 mol%. The impact of doping Eu3+ and photoluminescence properties were investigated and we propose a feasible interpretation. Copyright © 2012 John Wiley & Sons, Ltd.
Chemiluminescence determination of gemifloxacin based on diperiodatoargentate (III)‐sulphuric acid reaction in a micellar medium
Authors: Zhao Fang , Zhao Wen‐hui , Xiong Wei 
Published: February 2012

ABSTRACTA novel flow‐injection chemiluminescence (FI‐CL) analysis method for the determination of gemifloxacin in the presence of cetyltrimethylammonium bromide (CTAB) surfactant micelles is described. Strong CL signal was generated during the reaction of gemifloxacin with diperiodatoargentate (III) in a sulfuric acid medium sensitized by CTAB. Under optimum experimental conditions, the CL intensity was linearly related to the concentration of gemifloxacin from 1.0 × 10‐9 to 3.0 × 10‐7 g/mL and the detection limit was 7.3 × 10‐10 g/mL (3σ). The relative standard deviation (RSD) was 1.7 % for a 3.0 × 10‐8 g/mL gemifloxacin solution (11 repeated measurements). The proposed method was successfully applied to the determination of gemifloxacin in pharmaceutical preparations and biological fluids. The possible mechanism of the CL reaction is also discussed briefly. Copyright © 2012 John Wiley & Sons, Ltd.
Supramolecular Tandem Enzyme Assays
Authors: Dsouza Roy N. , Hennig Andreas , Nau Werner M. 
Published: February 2012

AbstractWe conceptualize a novel approach towards enzyme assays based on the reversible and competitive binding of a fluorescent dye and the substrate as well as product of an enzymatic reaction to a macrocyclic host. This method was termed “supramolecular tandem assay”, and has been applied to inhibitor and activator screening, sensor array development, and enantiomeric excess determination of amino acids. The simple and rapid read‐out by fluorescence allows their straightforward implementation into high‐throughput screening.
A Fluorescence‐Based Supramolecular Tandem Assay for Monitoring Lysine Methyltransferase Activity in Homogeneous Solution
Authors: Florea Mara , Kudithipudi Srikanth , Rei Ana , González‐Álvarez María José , Jeltsch Albert , Nau Werner M. 
Published: February 2012

AbstractThe demand for practical and convenient enzyme assays for histone lysine methyltransferases (HKMTs) emerges along with the rapid development of this young class of enzymes. A supramolecular reporter pair composed of p‐sulfonatocalix[4]arene (CX4) and the fluorescent dye lucigenin (LCG) has been used to monitor enzymatic trimethylation of lysine residues in peptide substrates. The assay affords a switch‐ON fluorescence response and operates in a continuous, real‐time, and label‐free fashion. The underlying working principle relies on the higher affinity of the macrocycle towards the trimethylated product of the enzymatic reaction as compared to the substrate, which allows the assay to be carried out in the product‐selective mode. The final product incorporates a trimethylammonium moiety, a known high‐affinity binding motif for CX4. Two substrates corresponding to the H3 N‐terminal tail, namely, S2 (RTKQTARKSTGGKAP) and S6 (QTARKSTGGS), were selected as model compounds for methylation with the Neurospora crassa Dim‐5 enzyme and investigated by the newly developed supramolecular tandem HKMTs assay. Only the longer substrate S2 underwent methylation in solution. The potential of the assay for inhibitor screening was demonstrated by means of inhibition studies with 1,10‐phenanthroline to afford an inhibition constant of (70±20) μM.
Excited State Properties and Energy Transfer within Dipyrrin‐Based Binuclear Iridium/Platinum Dyads: The Effect of ortho‐Methylation on the Spacer
Authors: Bronner Catherine , Veiga Manoel , Guenet Aurélie , De Cola Luisa , Hosseini Mir Wais , Strassert Cristian A. , Baudron Stéphane A. 
Published: February 2012

AbstractLuminescent cyclometalated iridium complexes based on pyridyl appended dipyrrin ligands were prepared and characterized both in the solid state and in solution. The functionalization of the peripheral pyridyl moiety causes dramatic changes on the emission properties of both mono‐ and hetero‐ binuclear complexes. A detailed photophysical investigation of the two mononuclear derivatives of the [(Ppy)2Ir(dpm‐py)] family (Ppy=2‐phenylpyridine, dpm‐py=5‐(4‐pyridyl)dipyrrin) was carried out. Introduction of methyl groups at the 3 and 5 positions on the pyridyl unit diminishes the non‐radiative rate constant by locking the peripheral pyridyl group orthogonally to the dipyrrinato plane. Thus, they limit the rotational degree of freedom, as well as the charge‐transfer character of the excited state. The coordination of these two complexes to a cyclometalated [(dppy)Pt] fragment (dppy=2,6‐diphenylpyridine) led to the formation of binuclear species in which the iridium and platinum complexes behave as acceptors and donors, respectively. In these heterobinuclear compounds, the methyl groups do not influence the energy transfer efficiency, which is estimated to be above 90 %. However, they do limit the charge‐transfer character of the acceptor’s excited state, as well as its rotational degree of freedom, thus avoiding the detrimental effect upon the photophysical performance.
Commentary on “Cervical cancer screening in England: The past, present, and future”: A comparison with Canada
Authors: Duggan Máire A. 
Published: February 2012

AbstractFor cervical cancer screening to be most effective, all eligible women in the population must be enrolled in an organized program. In this issue, Albrow et al describe how cervical cancer screening in England migrated from “opportunistic” in the 1960s to “organized” in the 1980s and discuss its continued evolution as new screening approaches and technologies developed. England's current National Health Services Cervical Screening Programme is the poster child for organized screening delivery. A similar model of central coordination and peripheral delivery of screening does not exist in Canada. The provision of health services is under provincial jurisdiction, and consequently each province administers its own version of a cervical cancer screening program. To date, organized screening has only been achieved in 2 of 10 provinces, and screening elsewhere is opportunistic. Screening in Canada begins at age 18‐21, ends at age 69‐70, and mostly occurs annually. This approach has yielded a significant reduction in cervical cancer incidence and mortality, but health service utilization costs have been high. The future should see full implementation of organized screening in all provinces, and when combined with the publicly funded human papillomavirus vaccination program of young girls begun in 2008, the result should be even better cancer control. Cancer (Cancer Cytopathol) 2012;. © 2011 American Cancer Society.
Cervical screening in England: The past, present, and future
Authors: Albrow Rebecca , Kitchener Henry , Gupta Nalini , Desai Mina 
Published: February 2012

AbstractCervical screening in England commenced in a disorganized fashion in 1964. The flaws of this approach became apparent in the mid‐1980s and led to the inception of the National Health Service Cervical Screening Programme (NHSCSP). The main features of this program are its population‐based registry, accessibility to all women within the screening age range, its systematic process of call and recall, national coordination, and quality assurance. Its success is in part based on its ability to evolve as evidence necessitates, and throughout the period of 2000‐2010, it embarked upon a series of developments involving liquid‐based cytology, which also provided the means to conduct reflex high‐risk human papillomavirus (HR‐HPV) testing and the potential to automate the screening process. As a result of evidence acquired since 2000, the NHSCSP is currently based on a system of primary cytology with HPV triage for low‐grade abnormalities combined with cytology plus a HR‐HPV “test of cure” for women who have received treatment for cervical intraepithelial neoplasia. Future challenges for the program will involve finding solutions to increasing screening uptake among women <30 years of age—a problem that may be exacerbated when vaccinated women reach the screening age, while making plans to accommodate HPV primary screening. Cancer (Cancer Cytopathol) 2012;. © 2012 American Cancer Society.
Detection of carbonic anhydrase IX protein in the diagnosis of malignant pleural effusion by enzyme‐linked immunosorbent assay and immunocytochemistry
Authors: Liao Nai‐Ding , Lee Wen‐Ying 
Published: February 2012

AbstractBACKGROUND:Carbonic anhydrase IX (CAIX) is a hallmark of metabolic change in cancer cells. The aim was to evaluate the utility of CAIX expression for the detection of malignant pleural effusions by enzyme‐linked immunosorbent assay (ELISA) and immunocytochemistry.METHODS:A total of 97 pleural effusions including 54 benign effusions, 10 atypical effusions, and 33 malignant effusions, classified based on cytological diagnosis and etiology, were subjected to ELISA to measure protein level and to immunocytochemistry in cell blocks to determine CAIX expression.RESULTS:CAIX levels were significantly higher in the malignant group compared with the benign group. Receiver operating characteristic curve analysis of benign and malignant pleural effusion for CAIX indicated an area under the curve of 0.82 with a value of 1882 pg/dL as the best threshold for distinguishing benign from malignant effusions, which yields a sensitivity, specificity, and accuracy of 63.6%, 94.4%, and 82.8%, respectively. None of the benign effusion expressed CAIX by immunocytochemistry. Malignant effusion cell blocks expressed CAIX in 63.6% of cases with 100% specificity. Upon combination of CAIX level and immunocytochemistry to detect malignant pleural effusions, the sensitivity and accuracy increased to 81.8% and 89.7%, respectively. The CAIX level determined by ELISA and the CAIX expression detected by immunocytochemistry were positively correlated. In atypical effusions, 5 cases (50%) exhibited CAIX levels higher than the cutoff value or were highlighted by CAIX immunocytochemistry.CONCLUSIONS:ELISA and immunocytochemistry methods for determination of CAIX protein are potential additional tests for detection of malignant pleural effusions. Cancer (Cancer Cytopathol) 2012;. © 2012 American Cancer Society.
Synthesis and Conformational Analysis of Methyl N‐Alanyl‐1′‐aminoferrocene‐1‐carboxylate
Authors: Barišić Lidija , Kovačević Monika , Mamić Marija , Kodrin Ivan , Mihalić Zlatko , Rapić Vladimir 
Published: February 2012

AbstractStructurally different ferrocene peptides I–VI exhibit considerable conformational differences. This study has explored the structural properties of VII [Y‐AA‐Fca‐OMe; Y = di‐tert‐butyl dicarbonate (Boc), acetyl (Ac); AA = L‐Ala, D‐Ala; Fca = 1′‐aminoferrocene‐1‐carboxylic acid] with an exchanged sequence of constituent amino acids relative to VI (Y‐Fca‐AA‐OMe; Y = Boc, Ac; AA = L‐Ala, D‐Ala). The ferrocene peptides VII were obtained by coupling C‐protected Fca with Boc‐L‐Ala‐OH and Boc‐D‐Ala‐OH, respectively. The Boc protecting groups of the obtained conjugates Boc‐AA‐Fca‐OMe (2a, AA = L‐Ala; 2b, AA = D‐Ala) were converted to sterically less demanding Ac groups to give Ac‐AA‐Fca‐OMe (3a, AA = L‐Ala; 3b, AA = D‐Ala). In order to examine their conformational properties, peptides VII were subjected to spectroscopic analysis (IR, 1H NMR, CD) and molecular modeling (DFT). The alteration of peptides VI into VII significantly influenced their conformational properties. Conjugates VI were stabilized through interchain hydrogen bonding, whereas inter‐ and intrachain hydrogen bonds were established in their constitutional isomers VII. The replacement of the bulky Boc group with an Ac group did not cause major conformational changes, and the absolute configuration of the alanine chiral centers did not affect the conformational properties.
A Unified Strategy for the Syntheses of Angucyclinone Antibiotics: Total Syntheses of Tetrangulol, Kanglemycin M, X‐14881‐E, and Anhydrolandomycinone
Authors: Vanga Devendar Goud , Kaliappan Krishna P. 
Published: February 2012

AbstractA unified strategy for the syntheses of angucyclinone antibiotics was developed utilizing sequential intramolecular enyne metathesis, Diels–Alder/aromatization, photooxygenation, and one‐pot elimination/aromatization reactions. The diversity in this sequence was introduced in the Diels–Alder reaction where a common diene was treated with various appropriately functionalized quinones as the dienophiles to accomplish the total syntheses of tetrangulol, kanglemycin M, X‐14881‐E, and anhydrolandomycinone in moderate to good overall yields. The requisite diene was synthesized in excellent yield from a known enyne through an intramolecular enyne metathesis. The scope of this flexible and divergent strategy can be extended to the syntheses of similar scaffolds and unnatural aromatic angucyclinones.
Lung endothelial ADAM17 regulates the acute inflammatory response to lipopolysaccharide
Authors: Dreymueller Daniela , Martin Christian , Kogel Tanja , Pruessmeyer Jessica , Hess Franz M. , Horiuchi Keisuke , Uhlig Stefan , Ludwig Andreas 
Published: February 2012

AbstractAcute lung injury (ALI) is associated with increased vascular permeability, leukocyte recruitment, and pro‐inflammatory mediator release. We investigated the role of the metalloproteinase ADAM17 in endotoxin‐induced ALI with focus on endothelial ADAM17. In vitro, endotoxin‐mediated induction of endothelial permeability and IL‐8‐induced transmigration of neutrophils through human microvascular endothelial cells required ADAM17 as shown by inhibition with GW280264X or shRNA‐mediated knockdown. In vivo, ALI was induced by intranasal endotoxin‐challenge combined with GW280264X treatment or endothelial adam17‐knockout. Endotoxin‐triggered upregulation of ADAM17 mRNA in the lung was abrogated in knockout mice and associated with reduced ectodomain shedding of the junctional adhesion molecule JAM‐A and the transmembrane chemokine CX3CL1. Induced vascular permeability, oedema formation, release of TNF‐α and IL‐6 and pulmonary leukocyte recruitment were all markedly reduced by GW280264X or endothelial adam17‐knockout. Intranasal application of TNF‐α could not restore leukocyte recruitment and oedema formation in endothelial adam17‐knockout animals. Thus, activation of endothelial ADAM17 promotes acute pulmonary inflammation in response to endotoxin by multiple endothelial shedding events most likely independently of endothelial TNF‐α release leading to enhanced vascular permeability and leukocyte recruitment.