bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and diagnostic application/evaluation of pandemic (H1N1) 2009 influenza virus‐specific monoclonal antibodies
Authors: Kim Won‐Shik , Lee Gyu‐Cheol , Yoo Jong‐Ha , Kim Hak‐Yong , Yun Yeo‐Pyo , Chong Chom‐Kyu 
Published: June 2012

ABSTRACTWe prepared mAb specific to the H1N1 2009 virus (H1N1 2009) to facilitate development of an RDT with enhanced sensitivity and specificity. Among these antibodies, we identified two clones—hybridomas 1H7E1 and 3A3H7—that specifically bound to H1N1 2009 (non‐seasonal) and were very suitable for application to a diagnostic kit. The affinity constants (Ka) of 1H7E1 and 3A3H7 were 1.10 × 1010and 2.35 × 1010, respectively. To identify the antibodies, we performed ELISA and immunoblot analyses and found that 1H7E1 recognized a conformational epitope of HA while 3A3H7 recognized a linear epitope. In clinical evaluations using specimens from 215 patients, a lateral flow rapid testing kit comprising these mAb showed a sensitivity of 81.5% (75/92) and a specificity of 96.7% (119/123). Results using the RDT kit were well correlated with conventional RT‐PCR methods as commonly and commercially used. Based on our findings, we believe that use of these mAb with a rapid evaluation kit could serve as a good diagnostic tool for H1N1 2009.
In non‐follicular lymphoproliferative disorders, IGH/BCL2‐fusion is not restricted to chronic lymphocytic leukaemia
Authors: Baseggio Lucile , Geay Marie‐Odile , Gazzo Sophie , Berger Françoise , Traverse‐Glehen Alexandra , Ffrench Martine , Hayette Sandrine , Callet‐Bauchu Evelyne , Verney Aurélie , Morel Dominique , Jallades Laurent , Magaud Jean‐Pierre , Salles Gilles , Felman Pascale 
Published: June 2012

SummaryThe translocation t(14;18) and its t(2;18) and t(18,22) variants, which involve the BCL2 genetic hallmark for follicular lymphoma (FL), have been reported in several cases of chronic B‐cell lymphoproliferative disease (CLPD) and frequently in chronic lymphocytic leukaemia (CLL). We describe here the clinical, morphological, immunological, cytogenetic and molecular findings from 37 cases of t(14;18)‐positive CLPD, identified from our series of non‐FL B‐cell neoplasms (n = 993) that were routinely analysed in peripheral blood by conventional cytogenetics analyses. The FL diagnosis was excluded by morphology and immunology (the samples were CD10 negative in all cases). The BCL2 translocations were observed in 22 CLL cases, including 7 monoclonal B‐cell lymphocytosis (MBL) cases re‐classified according to the new International Workshop on CLL criteria, six small lymphocytic lymphoma (SLL) cases, 1 splenic marginal zone lymphoma (SMZL) case and eight cases of unclassifiable CLPD with overlapping CLL/MZL features. In the CLL cases, the IGH/BCL2 fusion was remarkably associated with trisomy 12 (13/22) and mutated IGHV status (20/21) and did not affect the outcome. Moreover, most of these CLLs harboured a low mutation load of BCL6 gene and unmutated FAS (CD95) loci, which points to a post–germinal‐centre cellular origin.
Effect of different technological and culinary treatments on iron retention, nutritional density and recommended dietary intake in fourteen vegetable species
Authors: Korus Anna , Lisiewska Zofia , Słupski Jacek , Gębczyński Piotr 
Published: June 2012

SummaryThe present work determined iron content in fourteen species of vegetable prepared for consumption. The experimental material consisted of raw and boiled raw vegetables and two types of frozen product: one traditionally produced (blanching before freezing); the other obtained using the modified method (boiling before freezing), having the characteristics of a ready‐to‐eat convenience product. Nutrient density (ND) and recommended dietary intake (RDI) were established on the basis of iron content. The highest iron content was found in pea seeds (2.03 mg per 100 g fresh weight) and the lowest in root vegetables (0.38–0.60 mg). Iron retention was similar in all pea, broad bean, New Zealand spinach, kale, white cauliflower, celery and red beet products. In the remaining products, the significantly lowest retention was found in the traditionally frozen product (Sample C) and the highest in the ‘convenience’ frozen product (Sample D). The above‐mentioned order also applied to ND and RDI values. Only in the ND of parsnip calculated for women did any value fall below 100%.
Sodium chloride – sensory, preserving and technological impact on yeast‐leavened products
Authors: Beck Margit , Jekle Mario , Becker Thomas 
Published: June 2012

SummaryDietary sodium reduction is an important risk factor in the development of hypertension. As salted, yeast‐leavened products are a major source of dietary sodium, the baking industry needs to explore ways to reduce sodium in its products. Therefore, the impact of NaCl on shelf life, rheology and technological attributes as well as on the sensory quality of yeast‐leavened products needs to be understood. This review offers essential background knowledge about the effects of NaCl reduction on dough and yeast‐leavened products as well as techniques for reduction. Further, this review suggests paths of future research, including the overall impact of various NaCl levels on dough rheology and end product quality (yeast‐leavening rate, crumb texture, staling rate and retrogradation) and a global, detailed consideration of the effect of a comprehensive range of NaCl on the main flour components (starch and protein).
Compositional characteristics and aromatic profile of date palm seeds from seven varieties grown in Tunisia
Authors: Saafi‐Ben Salah Emna B. , Flamini Guido , El Arem Amira , Issaoui Manel , Dabbou Samia , BenYahia Leila , Ferchichi Ali , Hammami Mohamed , Achour Lotfi 
Published: June 2012

SummaryThis paper reports a discrimination study based on the physico‐chemical characteristics, fatty acids and profile of volatile compounds of the seeds from seven date palm varieties (Phoenix dactylifera L.) grown in Tunisia. Date seeds contained 10.49–14.76% moisture; 6.28–11.2% fat (on a dry weight basis); 2.67–12.85% protein; 0.91–6.06% reducing sugar; 0.61–2.98% sucrose and 0.97–1.17% ash. Gas liquid chromatography revealed that the oil fraction of the date palm seeds contained eighteen fatty acids, with oleic acid (30.77–42.50%) and lauric acid (18.51–27.48%) as the main unsaturated and saturated ones. Volatile profile showed differences among varieties. In total, forty‐five compounds were identified, mainly alcohols, aldehydes and unsaturated hydrocarbons ones. This study provides evidence that the seeds of date may be a potential source of valuable nutrients with interesting functionality.
Metal content of southern Italy honey of different botanical origins and its correlation with polyphenol content and antioxidant activity
Authors: Perna Annamaria , Simonetti Amalia , Intaglietta Immacolata , Sofo Adriano , Gambacorta Emilio 
Published: June 2012

SummarySeventy‐eight samples of southern Italy honey from five different floral origins (chestnut, eucalyptus, citrus, multifloral and sulla) were screened to quantify the polyphenol and metal contents, evaluate the antioxidant activity and determine the correlations between the parameters analysed. The average polyphenol content was 12.06 mg gallic acid equivalent per 100 g honey and 7.92 mg quercetin equivalent per 100 g honey, for total phenolic and flavonoid contents, respectively. The antioxidant activity ranged from 58.40% (eucalyptus honey) to 60.42% (chestnut honey) in the ABTS assay, from 152.65 μm Fe (II) (citrus honey) to 881.34 μm Fe (II) (chestnut honey) in the FRAP assay, and from 54.29% (citrus honey) to 78.73% (chestnut honey) in the DPPH assay. Fe and Zn were the most abundant among the tested metals, while Cd, Co and Mo were those less present. Chestnut honey presented the highest polyphenol content, antioxidant activity and metal content. The correlations between the analysed parameters were statistically significant (P < 0.05). The correlations between metal content and both total phenolic and antioxidant activities were particularly interesting, suggesting a relationship between metal and polyphenol contents in honey.
Relationship between growth of food‐spoilage yeast in high‐sugar environments and sensitivity to high‐intensity pulsed UV light irradiation
Authors: Hayes Jennifer C. , Laffey John G. , McNeil Brian , Rowan Neil J. 
Published: June 2012

SummaryThe relationship between prior growth of food‐spoilage yeast in high‐sugar environments and their subsequent survival postpulsed UV (PUV) irradiation was investigated. Test yeast were separately grown to early stationary phase in YPD broth containing increasing concentrations of glucose (1–50% w/v) and were flashed with ≤40 pulses of broad‐spectrum light at lamp discharge energy settings of 3.2, 7.2 and 12.8 J (equivalent to UV doses of 0.53, 1.09 and 3.36 μJ cm−2, respectively) and their inactivation measured. Findings showed that prior growth in high‐sugar conditions (≥30% glucose w/v) enhanced the sensitivity of all nine representative strains of Zygosaccharomyces bailii, Z. rouxii and Saccharomyces cerevisiae yeast to PUV irradiation. Significant differences in inactivation amongst different yeast types also occurred depending on amount of UV dose applied, where the order of increasing sensitivity of osmotically stressed yeast to PUV irradiation was shown to be Z. rouxii, Z. bailii and >S. cerevisiae. For example, a 1.2‐log order difference in CFU mL−1 reduction occurred between Z. bailii 11 486 and S. cerevisiae 834 when grown in 50% w/v sugar samples and treated with the uppermost test UV dosage of 3.36 μJ cm−2, where these two yeast strains were reduced by 3.8 and 5.0 log orders, respectively, after this PUV treatment regime compared to untreated controls. The higher the UV dose applied the greater the reduction in yeast numbers. For example, a 1.0‐, 1.4‐ and 4.0‐log order differences in CFU mL−1 numbers occurred for S. cerevisiae 834 grown in 15% w/v sugar samples and then treated with PUV dose of 0.53, 1.09 and 3.36 μJ cm−2, respectively. These findings support the development of PUV for the treatment of high‐sugar foods that are prone to spoilage by osmotolerant yeast.
The role of role: learning in longitudinal integrated and traditional block clerkships
Authors: Hauer Karen E , Hirsh David , Ma Iris , Hansen Lori , Ogur Barbara , Poncelet Ann N , Alexander Erik K , O’Brien Bridget C 
Published: June 2012

Medical Education 2012: 46:698–710Context  Traditional block clerkship (BC) structures may not optimally support medical student participation in the workplace, whereas longitudinal integrated clerkship (LIC) structures seem more conducive to students’ active engagement in patient care over time. Understanding the ways in which these two clerkship models influence students’ roles and responsibilities can inform clinical learning programme design.Methods  This was a multicentre qualitative study. We conducted semi‐structured interviews with LIC and BC medical students at three institutions early and late in the core clinical year to explore their experiences with patients and the roles they served. Using the framework of ‘workplace affordances’, qualitative coding focused on students’ roles and qualities of the learning environment that invited or inhibited student participation. We compared transcripts of early‐ and late‐year interviews to assess students’ changing roles and conducted discrepant case analysis to ensure that coding fit the data.Results  Fifty‐four students participated in interviews. They described serving three major roles in clinical care that respectively involved: providing support to patients; sharing information about patients across health care settings, and functioning in a doctor‐like role. Both LIC and BC students served in the providing support and transmitting information roles both early and late in the year. By contrast, LIC students commonly served in the doctor‐like role in managing their patients’ care, particularly late in the year, whereas BC students rarely served in this role. Continuity in settings and in supervisors, and preceptors’ endorsement of students’ legitimate role afforded opportunities for students to participate actively in patient care.Conclusions  Although both LIC and BC students reported serving in important roles in supporting their patients and sharing information about their care, only LIC students consistently described opportunities to grow into a doctor role with patients. The high level of integration of LIC students into care systems and their deeper relationships with preceptors and patients enhanced their motivation and feelings of competence to provide patient‐centred care.
Road to Royalty – Transition of Potential Queen to Queen in the Primitively Eusocial Wasp Ropalidia marginata
Authors: Mitra Aniruddha , Gadagkar Raghavendra 
Published: June 2012

Abstract

Ropalidia marginata, a primitively eusocial wasp, is different from typical primitively eusocial species in having docile queens who cannot be using dominance to maintain reproductive monopoly and instead appear to use a pheromone from the Dufour's gland to do so. When a docile queen is removed from her colony, one of the workers (potential queen, PQ) becomes highly aggressive, and if the queen is not returned, gradually loses her aggression and becomes the new docile queen within a few days. We hypothesized that the decrease in aggression of the PQ with time since queen removal should be correlated with her change in ovaries and pheromone profile. Because the Dufour's gland hydrocarbon composition in
R. marginata can be correlated with fertility, this also gave us an opportunity to test whether PQ is different from workers in her Dufour's gland hydrocarbons. In this study, we therefore trace the road to royalty in
R. marginata, that is, the transition of the PQ during queen establishment, in terms of her ovaries, aggression, and Dufour's gland hydrocarbons. Our study focuses on queen establishment, which is important for understanding how reproductive conflict can be manifested and resolved.
Development of a co‐dominant DNA marker linked to the gene lentus conferring reduced pod shattering for marker‐assisted selection in narrow‐leafed lupin (Lupinus angustifolius) breeding
Authors: Li Xin , Yang Huaan , Yan Guijun 
Published: June 2012

With 1 figure and 4 tablesAbstractMarker‐assisted selection (MAS) is desirable for breeding non‐shattering pods in narrow‐leafed lupin and for facilitating the process of broadening the gene pool of existing domesticated Australian lupin cultivars. In this study, we developed a molecular marker using a strategy to incorporate the validation step during the candidate marker identification step by microsatellite‐anchored fragment length polymorphisms (MFLP). Three MFLP polymorphisms showing banding patterns consistent with pod‐shattering phenotypes on the 12 representative F8 recombinant inbred lines (RILs) were identified as candidate markers linked to lentus. One of these three markers matching the lentus phenotypes on representative cultivars and wild accessions was selected and converted into a co‐dominant PCR‐based marker, named LeLi. When marker LeLi was applied to 25 cultivars released in Australia and 125 wild core accessions of the Australian Lupin Collection, an overall matching rate of 60.67% was found assuming correct determination of the phenotypes. This newly developed marker is simple PCR‐based and co‐dominant and can be used for MAS in current lupin breeding in Western Australia.
Stent patency using competing risk model in unresectable pancreatic cancers inserted with biliary self‐expandable metallic stent
Authors: Eum Young Ook , Kim Yong‐Tae , Lee Sang Hyub , Park Sang Wook , Hwang Jin‐Hyeok , Yoon Won Jae , Ryu Ji Kon , Yoon Yong Bum , Han Joon Koo , Yoon Chang Jin , Cho Jung‐Hwa , Choi Yunhee 
Published: June 2012

Background and aimBiliary self‐expandable metallic stents (SEMS) play an important role in the quality of life and palliative treatment in unresectable pancreatic cancer patients. We aimed to determine the factors affecting the patency of biliary SEMS and the survival in unresectable pancreatic cancer with obstructive jaundice.MethodsConsidering the competing risk and survival, we retrospectively evaluated the patency in 107 unresectable pancreatic cancer patients with obstructive jaundice who were successfully treated with biliary SEMS from January 2000 to April 2010.ResultsThere were 107 incidents of biliary drainage that were clinically successful and the overall survival period was a median of 133 days. Stent occlusion before death was observed in 36 (33.6%) of 107 patients. Cumulative stent obstruction rates were4.7%, 16.8%, and 24.4% at 1, 3, and 6 months, respectively. Lower cancer stage (<5 month's hazard ratio [HR] = 2.327, >5 month's HR = 0.108) was only associated with the longer patency of the stents in a multivariable analysis using a Fine and Gray model that considered competing risk. In multivariable analysis, lower cancer stage, uncovered stent and normalized serum bilirubin level were associated with a longer survival period (HR = 2.335, 1.906 and 1.795 respectively, P < 0.05).ConclusionThe patency of biliary SEMS in unresectable pancreatic cancers might be affected by the stage. Lower cancer stage and normalized bilirubin are associated with longer survival.
Association between physical performance characteristics and independence in activities of daily living in middle‐aged and elderly men
Authors: den Ouden Marjolein EM , Schuurmans Marieke J , Arts Ilse EMA , van der Schouw Yvonne T 
Published: June 2012

Aim:  Functional status at one moment in time is a determinant of future functional status and survival. Physical deterioration tends to occur early in the disabling process; however, etiological questions remain. This study investigated the association between physical performance characteristics and functioning independently in middle‐aged and elderly men.Methods:  A total of 400 independently‐living men aged 40–80 years were included in this cross‐sectional study. Preservation of function was measured using the Stanford Health Assessment Questionnaire. Physical characteristics were muscle strength and power by dynamometer, lung function, lower extremity function by physical performance score, and physical activity by Voorrips‐questionnaire. Logistic regression analysis was used to estimate the association between potential determinants and the dichotomized Health Assessment Questionnaire score. The odds ratios (OR) were adjusted for age, body mass index, education, socioeconomic status, smoking, alcohol and number of chronic diseases.Results:  After adjustment for confounders, higher walking speed (OR = 2.96, 95% CI 1.31–6.72) and shorter time to carry out the chair stand test (OR = 0.84, 95% CI 0.76–0.94) were associated with a higher probability of being independent in activities of daily living (ADL). Borderline significant associations were found for higher lung function and higher leg strength with higher probability of being independent in ADL. No associations were found for grip strength, physical performance score, standing balance and physical activity.Conclusion:  Lower body function and lung function were associated with a higher probability of being independent in ADL. Geriatr Gerontol Int 2013; 13: 274–280.
Archaeal amoA gene diversity points to distinct biogeography of ammonia‐oxidizing Crenarchaeota in the ocean
Authors: Sintes Eva , Bergauer Kristin , De Corte Daniele , Yokokawa Taichi , Herndl Gerhard J. 
Published: June 2012

SummaryMesophilic ammonia‐oxidizing Archaea (AOA) are abundant in a diverse range of marine environments, including the deep ocean, as revealed by the quantification of the archaeal amoA gene encoding the alpha‐subunit of the ammonia monooxygenase. Using two different amoA primer sets, two distinct ecotypes of marine Crenarchaeota Group I (MCGI) were detected in the waters of the tropical Atlantic and the coastal Arctic. The HAC‐AOA ecotype (high ammonia concentration AOA) was ≈ 8000 times and 15 times more abundant in the coastal Arctic and the top 300 m layer of the open equatorial Atlantic, respectively, than the LAC‐AOA (low ammonia concentration AOA) ecotype. In contrast, the LAC‐AOA ecotype dominated the lower meso‐ and bathypelagic waters of the tropical Atlantic (≈ 50 times more abundant than the HAC‐AOA) where ammonia concentrations are well below the detection limit using conventional spectrophotometric or fluorometric methods. Cluster analysis of the sequences from the clone libraries obtained by the two amoA primer sets revealed two phylogenetically distinct clusters. Taken together, our results suggest the presence of two ecotypes of archaeal ammonia oxidizers corresponding to the medium (1.24 µM on average in the coastal Arctic) and low ammonia concentration (< 0.01 µM) in the shallow and the deep waters respectively.
An ABC transporter and an outer membrane lipoprotein participate in posttranslational activation of type VI secretion in Pseudomonas aeruginosa
Authors: Casabona Maria G. , Silverman Julie M. , Sall Khady M. , Boyer Frédéric , Couté Yohann , Poirel Jessica , Grunwald Didier , Mougous Joseph D. , Elsen Sylvie , Attree Ina 
Published: June 2012

SummaryPseudomonas aeruginosa is capable of injecting protein toxins into other bacterial cells through one of its three type VI secretion systems (T6SSs). The activity of this T6SS is tightly regulated on the posttranslational level by phosphorylation‐dependent and ‐independent pathways. The phosphorylation‐dependent pathway consists of a Threonine kinase/phosphatase pair (PpkA/PppA) that acts on a forkhead domain‐containing protein, Fha1, and a periplasmic protein, TagR, that positively regulates PpkA. In the present work, we biochemically and functionally characterize three additional proteins of the phosphorylation‐dependent regulatory cascade that controls T6S activation: TagT, TagS and TagQ. We show that similar to TagR, these proteins act upstream of the PpkA/PppA checkpoint and influence phosphorylation of Fha1 and, apparatus assembly and effector export. Localization studies demonstrate that TagQ is an outer membrane lipoprotein and TagR is associated with the outer membrane. Consistent with their homology to lipoprotein outer membrane localization (Lol) components, TagT and TagS form a stable inner membrane complex with ATPase activity. However, we find that outer membrane association of T6SS lipoproteins TagQ and TssJ1, and TagR, is unaltered in a ΔtagTS background. Notably, we found that TagQ is indispensible for anchoring of TagR to the outer membrane fraction. As T6S‐dependent fitness of P. aeruginosa requires TagT, S, R and Q, we conclude that these proteins likely participate in a trans‐membrane signalling pathway that promotes H1‐T6SS activity under optimal environmental conditions.