bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interleukin-10–Mediated Heme Oxygenase 1–Induced Underlying Mechanism in Inflammatory Down-Regulation by Norfloxacin in Cirrhosis §δ
Authors: Gómez-Hurtado Isabel , Zapater Pedro , Bellot Pablo , Pascual Sonia , Pérez-Mateo Miguel , Such José , Francés Rubén 
Published: December 2010

Abstract

Patients with cirrhosis receiving norfloxacin show a restored inflammatory balance that likely prevents clinical complications derived from an excessive proinflammatory response to bacterial product challenges. This study sought to investigate associated inflammatory control mechanisms established in patients with cirrhosis receiving norfloxacin. A total of 62 patients with cirrhosis and ascites in different clinical conditions were considered. Blood samples were collected and intracellular and serum norfloxacin were measured. Inflammatory mediators were evaluated at messenger RNA and protein levels. Neutrophils from all patients were cultured with lipopolysaccharide (LPS) and anti–interleukin-10 (anti–IL-10) monoclonal antibody in different conditions. IL-10 and heme oxygenase-1 (HO-1) were up-regulated in patients receiving norfloxacin and correlated with norfloxacin in a concentration-dependent manner, whereas proinflammatory inducible nitric oxide synthase, cyclooxygenase-2, and nuclear factor-κB behaved inversely. Higher IL-10 levels correlated with lower white blood cell count and higher mean arterial pressure. No correlations were found between IL-10 and disease clinical scores or liver function markers in blood. Neutrophilic

in vitro

assays showed that the effect of LPS on proinflammatory mediator levels in the presence of norfloxacin was abrogated by significantly increasing IL-10 and HO-1 expression. After stimulation with LPS plus anti–IL-10, proinflammatory mediators were dramatically increased in patients receiving norfloxacin, and increasing intracellular norfloxacin concentrations did not decrease the expression levels of these proinflammatory molecules. Unblocking IL-10 restored proinflammatory mediator and HO-1 expression to previously observed levels in response to LPS stimulation.

Conclusion:

Although the described association does not necessarily mean causality, an IL-10–mediated HO-1–induced anti-inflammatory mechanism is present in patients with cirrhosis receiving norfloxacin, that is directly associated with cell-modulating events in these patients. (Hepatology 2011;)
Bioluminescence reporter gene imaging characterize human embryonic stem cell‐derived teratoma formation
Authors: Su Weijun , Zhou Manqian , Zheng Yizhou , Fan Yan , Wang Lina , Han Zhongchao , Kong Deling , Zhao Robert Chunhua , Wu Joseph C. , Xiang Rong , Li Zongjin 
Published: December 2010

AbstractHuman embryonic stem (hES) cells have a potential use for the repair and regeneration of injured tissues. However, teratoma formation can be a major obstacle for hES‐mediated cell therapy. Therefore, tracking the fate and function of transplanted hES cells with noninvasive imaging could be valuable for a better understanding of the biology and physiology of teratoma formation. In this study, hES cells were stably transduced with a double fusion reporter gene consisting of firefly luciferase and enhanced green fluorescent protein. Following bioluminescence imaging and histology, we demonstrated that engraftment of hES cells was followed by dramatically increasing signaling and led to teratoma formation confirmed by histology. Studies of the angiogenic processes within teratomas revealed that their vasculatures were derived from both differentiated hES cells and host. Moreover, FACS analysis showed that teratoma cells derived from hES cells expressed high levels of CD56 and SSEA‐4, and the subcultured SSEA‐4+ cells showed a similar cell surface marker expression pattern when compared to undifferentiated hES cells. We report here for the first time that SSEA‐4+ cells derived from teratoma exhibited multipotency, retained their differentiation ability in vivo as confirmed by their differentiation into representative three germ layers. J. Cell. Biochem. 112: 840–848, 2011. © 2010 Wiley‐Liss, Inc.
Effect of desacyl ghrelin, obestatin and related peptides on triglyceride storage, metabolism and GHSR signaling in 3T3‐L1 adipocytes
Authors: Miegueu Pierre , St Pierre David , Broglio Fabio , Cianflone Katherine 
Published: December 2010

AbstractAcyl‐ghrelin (AG), desacyl‐ghrelin (DAG) and obestatin are all derived from the same gene transcript; however their plasma levels do not necessarily change in parallel. The influence of these peptides towards the development of obesity and their direct effects on adipocyte physiology has not been thoroughly investigated. This study was designed to evaluate the direct effects of peptides of the ghrelin family on preadipocyte proliferation, differentiation and adipocyte lipid and glucose metabolism in 3T3‐L1 cells. 3T3 cells were treated with physiological peptide concentrations for 1 h to 9 days, and the relevant assays measured. In preadipocytes, AG, GHRP‐6 and DAG stimulated proliferation, measured as 3H‐thymidine incorporation (up to 200%, P < 0.05), while all peptides stimulated differentiation (up to 300%, P < 0.01) as compared to standard differentiation conditions. In adipocytes, FA uptake was increased in a concentration‐dependent manner especially with obestatin (three‐ to fourfold, P < 0.001) and DAG (three‐ to fivefold, P < 0.001). By contrast, glucose transport was unchanged. DAG and obestatin significantly decreased lipolysis measured as non‐esterified fatty acid and glycerol release by 50%, P < 0.05–0.01 and 51%, P < 0.01, respectively. Interestingly, DAG stimulation of FA uptake was blocked with GHSR1 antagonist (D‐lys3)‐GHRP‐6 (P < 0.05), phospholipase C inhibitor U73122 and phosphatidylinositol‐3‐kinase inhibitor wortmannin (P < 0.001). Finally, in omental but not subcutaneous human adipose tissue, GHSR1 correlated with BMI (r = 0.549, P < 0.05) and insulin (r = 0.681, P < 0.01). Taken together, these results suggest that ghrelin‐related peptides may directly affect adipose tissue metabolism. J. Cell. Biochem. 112: 704–714, 2011. © 2010 Wiley‐Liss, Inc.
Nephroblastoma overexpressed (Nov) induces gremlin in ST‐2 stromal cell lines by post‐transcriptional mechanisms
Authors: Smerdel‐Ramoya Anna , Zanotti Stefano , Canalis Ernesto 
Published: December 2010

AbstractNephroblastoma overexpressed (Nov) inhibits osteoblastogenesis in part because it binds bone morphogenetic protein (BMP)‐2. In the present study, we investigated whether Nov regulated the expression of the BMP antagonist gremlin. Overexpression of Nov increased gremlin mRNA levels in ST‐2 cells, and its downregulation by RNA interference decreased gremlin mRNA. Nov did not affect Grem1 transcription, but prolonged the half‐life of gremlin mRNA in ST‐2 cells, demonstrating that Nov acts by post‐transcriptional mechanisms. This was confirmed by demonstrating that downregulation of Nov destabilizes gremlin transcripts. To assess whether the 3′‐untranslated region (UTR) of gremlin mRNA mediated the effect of Nov, the decay of a chimeric cfos gremlin 3′‐UTR construct was compared to that of cfos in ST‐2 cells. The presence of the gremlin 3′‐UTR prolonged the half‐life of cfos and was responsible for the effect of Nov. To examine the binding of the gremlin 3′‐UTR to ribonucleoproteins, radiolabeled gremlin RNA fragments were incubated with cytosolic extracts from Nov overexpressing and control cells. RNA electrophoretic mobility analysis revealed that Nov enhanced the binding of cytosolic proteins to the fragments spanning the 3′‐UTR of gremlin between bases 1,358–1,557 and 1,158–1,357 from the transcriptional start. Mutations of AU‐rich elements in these two RNA fragments prevented the formation of RNA–protein complexes induced by Nov. Nov did not alter the binding of cytosolic extracts to sequences present in the 5′‐UTR or coding region of gremlin. In conclusion, Nov stabilizes gremlin transcripts, and this effect is possibly mediated by AU‐rich elements present in the 3′‐UTR of gremlin. J. Cell. Biochem. 112: 715–722, 2011. © 2010 Wiley‐Liss, Inc.
RASSF1A suppresses melanoma development by modulating apoptosis and cell‐cycle progression
Authors: Yi Mei , Yang Jianbo , Chen Xiang , Li Ji , Li Xiayu , Wang Li , Tan Yixin , Xiong Wei , Zhou Ming , McCarthy James B. , Li Guiyuan , Xiang Bo , Xie Hongfu 
Published: December 2010

AbstractThe tumor suppressor candidate gene Ras association domain family 1, isoform A (RASSF1A) encodes a microtubule‐associated protein that is implicated in the regulation of cell proliferation, migration, and apoptosis. Several studies indicate that down‐regulation of RASSF1A resulting from promoter hypermethylation is a frequent epigenetic abnormality in malignant melanoma. In this study, we report that compared with melanocytes in normal skins or benign skin lesions, RASSF1A is down‐regulated in melanoma tissues as well as cell lines, and its expression negatively correlates with lymph node metastasis. Following ectopic expression in RASSF1A‐deficient melanoma A375 cell line, RASSF1A reduces cell viability, suppresses cell‐cycle progression but enhances apoptotic cell death. In vivo, RASSF1A expression inhibits the tumorigenic potential of A375 cells in nude mice, which also correlates with decreased cell proliferation and increased apoptosis. On the molecular level, ectopic RASSF1A expression leads to differential expression of 209 genes, including 26 down‐regulated and 183 up‐regulated ones. Among different signaling pathways, activation of the apoptosis signal‐regulating kinase 1 (ASK1)/p38 MAP kinase signaling is essential for RASSF1A‐induced mitochondrial apoptosis, and the inhibition of the Akt/p70S6 kinase/eIF4E signaling is also important for RASSF1A‐mediated apoptosis and cell‐cycle arrest. This is the first study exploring the biological functions and the underlying mechanisms of RASSF1A during melanoma development. It also identifies potential targets for further diagnosis and clinical therapy. J. Cell. Physiol. 226: 2360–2369, 2011. © 2010 Wiley‐Liss, Inc.
Obstetrical sonography in obese women: A review
Authors: Weichert Jan , Hartge David Rafael 
Published: December 2010

AbstractObstetric sonographic imaging in pregnant women is adversely affected by obesity with a negative impact on the detection rate of congenital anomalies. This review aims to analyze relevant data regarding this issue and to discuss clinical and technical problems associated with sonographic examination of obese pregnant women. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound, 2011
Radiologic findings of lobular carcinoma in situ: Mammography and ultrasonography
Authors: Choi Bo Bae , Kim Sung Hun , Park Chang Suk , Cha Eun Suk , Lee Ah Won 
Published: December 2010

AbstractPurpose. The purpose was to evaluate the mammographic and sonographic (US) features of lobular carcinoma in situ (LCIS).Methods. Mammographic and US findings of nine lesions diagnosed pathologically as pure LCIS were analyzed retrospectively according to the American College of Radiology breast imaging reporting and data system (BI‐RADS) lexicon.Results. With regards to mammographic findings of LCIS, there were no lesions demonstrated in six cases and a mass in three cases, two of which contained microcalcifications. The most common US findings of LCIS were irregular shape (five cases), ill‐defined margins (eight cases), and hypoechogenicity (seven cases). All cases had an elongated shape parallel to the skin or were round (no lesion had a taller‐than‐wide shape). Two cases were associated with microcalcifications. The final BI‐RADS categories were category 3 (probably benign finding) in one case, category 4A (low suspicion of malignancy) in two cases, and category 4B (intermediate suspicion of malignancy) in six cases.Conclusions. LCIS is frequently mammographically occult, and an incidental finding on routine screening mammograms, usually because of microcalcifications. LCIS, a high‐risk lesion, can mimic invasive carcinoma on US. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound, 2010
Biomechanical properties of interosseous proximal carpal row ligaments
Authors: Nikolopoulos Fotios , Apergis Emmanuel , Kefalas Vassilios , Zoubos Aristides , Soucacos Panayiotis , Papagelopoulos Panayiotis 
Published: December 2010

AbstractThe Scapholunate (S‐L) and Lunotriquetrum (L‐Tr) ligaments have been extensively studied in the literature. A wide range of measurements has been reported for ultimate load and stiffness with different mechanical protocols. In this study, we examined the mechanical properties of both ligaments harvested from the same wrist. Fifteen fresh cadaver wrists were used to harvest eight S‐L and four L‐Tr. Testing was performed in quasi‐static loading in a well defined direction for each ligament system. The ultimate load for S‐L was 68–210 N with a mean value of 147 ± 54 N and a stiffness of 35.7 ± 9.6 N/mm. For L‐Tr the ultimate load was 122–179 N with a mean value of 150 ± 24 N and a stiffness of 192 ± 60 N/mm. The two ligaments had nearly the same ultimate load, but the L‐Tr had a higher stiffness (p = 0.05). These findings could be useful to assess the appropriate autologous autografts for reconstruction of the S‐L and L‐Tr. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:668–671, 2011
Comparative oxidative stability of α‐tocopherol blended and diffused UHMWPEs at 3 years of real‐time aging
Authors: Rowell Shannon L. , Oral Ebru , Muratoglu Orhun K. 
Published: December 2010

AbstractVitamin E (α‐tocopherol) is a free‐radical stabilizing antioxidant used to maintain oxidative stability in radiation cross‐linked ultra‐high molecular weight polyethylene (UHMWPE) used in total joint replacements. We investigated the oxidative resistance of vitamin E‐stabilized UHMWPE in (i) postirradiation vitamin E‐diffused UHMWPE, (ii) vitamin E blended and irradiated UHMWPE, and (iii) unstabilized, irradiated UHMWPE after accelerated aging and real‐time aging in an aqueous environment at 40°C for 36 months. Unstabilized samples exhibited substantial oxidation throughout the surface and bulk with both types of aging. While vitamin E‐stabilized, radiation cross‐linked UHMWPEs were all superior to unstabilized samples, irradiated blends showed surface oxidation and subsurface oxidation potential beginning at ten months in real‐time aging. In contrast, postirradiation vitamin E‐diffused UHMWPEs showed no detectable oxidation and no increase in oxidation potential despite elution of some vitamin E. We also showed that current thermal accelerated aging methods were unable to differentiate among the oxidative stability of vitamin E‐stabilized, radiation cross‐linked UHMWPEs prepared by different processes. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:773–780, 2011
Effects of three commercial rootstocks on mineral nutrition, fruit yield, and quality of salinized tomato
Authors: Savvas Dimitrios , Savva Andreas , Ntatsi Georgia , Ropokis Andreas , Karapanos Ioannis , Krumbein Angelika , Olympios Christos 
Published: December 2010

AbstractTomato (Solanum lycopersicum Mill. cv. Belladona F1) plants were either self‐rooted, self‐grafted, or grafted onto the commercial rootstocks “Beaufort”, “He‐Man”, and “Resistar” and grown in a recirculating hydroponic system. Three nutrient solutions differing in NaCl‐salinity level (2.5, 5.0, and 7.5 dS m–1, corresponding to 0.3, 22, and 45 mM NaCl) were combined with the five grafting treatments in a two‐factorial (3 × 5) experimental design. At the control NaCl level (0.3 mM), fruit yield was not influenced by any of the grafting treatments. However, at low (22 mM NaCl) and moderate (45 mM NaCl) salinity levels, the nongrafted and the self‐grafted plants gave significantly lower yields than the plants grafted onto He‐Man. The plants grafted onto the other two rootstocks gave higher yields only in comparison with the nongrafted plants, and the differences were significant only at low (Beaufort) or moderate (Resistar) salinity. Yield differences between grafting treatments at low and moderate salinity arose from differences in fruit number per plant, while mean fruit weight was not influenced by grafting or the rootstock. NaCl salinity had no effect on the yield of plants grafted onto He‐Man but restricted the yield in all other grafting treatments due to reduction of the mean fruit weight. With respect to fruit quality, salinity enhanced the titratable acidity, the total soluble solids, and the ascorbic acid concentrations, while grafting and rootstocks had no effect on any quality characteristics. The leaf Na concentrations were significantly lower in plants grafted onto the three commercial rootstocks, while those of Cl were increased by grafting onto He‐Man but not altered by grafting onto Beaufort or Resistar in comparison with self‐grafted or nongrafted plants. Grafting onto the three tested commercial rootstocks significantly reduced the leaf Mg concentrations, resulting in clear Mg‐deficiency symptoms 19 weeks after planting.
Raman study of a deuterated iron hydroxycarbonate to assess long‐term corrosion mechanisms in anoxic soils
Authors: Saheb M. , Neff D. , Bellot‐Gurlet L. , Dillmann P. 
Published: December 2010

AbstractIn several contexts such as cultural heritage, oil and gas or nuclear waste disposal, the long‐term corrosion mechanisms of iron in anoxic soils are studied. For this purpose, corrosion layers formed on ferrous archaeological artefacts from the site of Glinet (16th century, Normandy, France) were characterised. The main phases identified are siderite (FeCO3), chukanovite (iron hydroxycarbonate: Fe2(OH)2CO3 and magnetite (Fe3O4). In order to provide reliable Raman references for further studies on carbonated systems, the iron hydroxycarbonate (chukanovite) was synthesised on iron discs. The corrosion mechanisms were investigated by re‐corroding the archaeological samples in a deuterated solution. Raman characterisation on cross sections inside the layer revealed the presence of deuterated chukanovite, allowing the deuterium tracing of the spreading of the corrosion. A set of chukanovite samples was synthesised with various D/H ratios. Using these reference data, the proportion of deuterated chukanovite in re‐corroded artefacts was evaluated, and the corrosion rate was estimated as less than 1.6 µm/year. Copyright © 2010 John Wiley & Sons, Ltd.
The growth of the passive film on iron in 0.05 M NaOH studied in situ by Raman micro‐spectroscopy and electrochemical polarisation. Part I: near‐resonance enhancement of the Raman spectra of iron oxide and oxyhydroxide compounds
Authors: Nieuwoudt M. K. , Comins J. D. , Cukrowski I. 
Published: December 2010

AbstractRaman spectroscopy, in principle, is an excellent technique for the study of molecular species developed on metal surfaces during electrochemical investigations. However, the use of the more common laser wavelengths such as the 514.5‐nm line results in spectra of less than optimal intensity, particularly for iron oxide compounds. In the present work, near‐resonance enhancement of the Raman spectra was investigated for the iron oxide and iron oxyhydroxide compounds previously reported to be present in the passive film on iron, using a tuneable dye laser producing excitation wavelengths between 560 and 637 nm. These compounds were hematite (α‐Fe2O3), maghemite (γ‐Fe2O3), magnetite (Fe3O4), goethite (α‐FeOOH), akaganeite (β‐FeOOH), lepidocrocite (γ‐FeOOH) and feroxyhyte (δ‐FeOOH). Optimum enhancement, when compared to that with the 514.5‐nm line, was obtained for all the iron oxide and oxyhydroxide standard samples in the low wavenumber region (<1000 cm−1) using an excitation wavelength of 636.4 nm. Particularly significant enhancement was obtained for lepidocrocite, hematite and goethite. Copyright © 2010 John Wiley & Sons, Ltd.
Comparison of molecular images as defined by Raman spectra between normal mucosa and squamous cell carcinoma in the oral cavity
Authors: Guze K. , Short M. , Zeng H. , Lerman M. , Sonis S. 
Published: December 2010

AbstractRaman spectroscopy has been effectively applied to clinically differentiate normal and cancerous mucosal tissues. Micro‐Raman spectroscopy provides a tool to better understand the molecular basis for the Raman clinical signal. The objective of the current study was to utilize micro‐Raman spectroscopy to define the molecular/spectral differences between normal and abnormal squamous cell carcinoma (SCC) in oral mucosa (in vitro). Understanding this may help in identifying unique spectra or may be useful for in vivo application of this technology. Micro‐Raman (confocal) spectroscopy was used to obtain molecular images of normal and SCC cells of human oral mucosa. Four fresh flashed‐frozen tumor and four matched normal tongue specimens were studied. The spectra covered a wavenumber range from 300 to 4000 cm−1 with a spectral resolution of 8 cm−1 and a spatial resolution of 1.0 µm. The cells were located within thin sections of tongue mucosa biopsies. The excitation wavelength of 515 nm was used. We were able to obtain Raman images with rich information about the spectroscopic and structural features within the cytoplasm, cell membrane, and cell nuclei. Significant spectral differences were observed between the Raman images of normal and malignant squamous cells. The heterogeneity of tumor cells within the abnormal tissue was also demonstrated. Spectral differences demonstrated between both tissue types have provided important information regarding the origins of specific signals within the cells of each tissue type. In our search for specific spectral biomarkers, we believe that a cell surface protein, greatly upregulated in SCC cells, was discovered at 1583 cm−1. Copyright © 2010 John Wiley & Sons, Ltd.
Diagnostic value of FDG‐PET for lymph node metastasis and outcome of surgery for biliary cancer
Authors: Kobayashi Shogo , Nagano Hiroaki , Hoshino Hiromitsu , Wada Hiroshi , Marubashi Shigeru , Eguchi Hidetoshi , Takeda Yutaka , Tanemura Masahiro , Kim Tonsok , Shimosegawa Eku , Hatazawa Jun , Doki Yuichiro , Mori Masaki 
Published: December 2010

AbstractBackground and ObjectivesHistopathologically confirmed lymph node metastasis is a prognostic factor in the surgical treatment of biliary tract cancer, however, preoperative diagnosis is still difficult even with computed tomography. FDG‐PET has been used for the diagnosis of cancer and metastatic lesions. Herein, we retrospectively evaluated the utility of FDG‐PET for detection of lymph node metastasis in biliary tract cancer.MethodsWe measured SUVmax at each 190 surgically dissected lymph node area in 36 patients, and compared the values with histopathological diagnosis. The cutoff values for SUVmax were defined from the ROC curve and the mean plus two standard deviations then used for detection of metastatic lymph node and prognostic value, compared with CT diagnosis.ResultsThe sensitivity, specificity, and positive predictive value of FDG‐PET were better than CT diagnosis (86%, 74%, 43% for SUVmax ≥ 2.0, and 37%, 97%, 72% for SUVmax ≥ 2.8, respectively). There was no relationship between SUVmax and CT‐determined lymph node dimensions. The presence of SUVmax ≥ 2.8 lymph nodes was an independent determinant of prognosis after surgical treatment.ConclusionThe detection of metastatic lymph nodes by FDG‐PET is limited, but better than CT. SUVmax for lymph nodes seems useful for clinical decision‐making regarding treatment strategy including surgery. J. Surg. Oncol. 2011; 103:223–229. © 2010 Wiley‐Liss, Inc.