bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long‐lasting immunoprotective and therapeutic effects of a hyperstable E7 oligomer based vaccine in a murine human papillomavirus tumor model
Authors: Cerutti María L. , Alonso Leonardo G. , Tatti Silvio , de Prat‐Gay Gonzalo 
Published: July 2011

AbstractCervical cancer and many other anogenital and oropharyngeal carcinomas are strongly associated with high‐risk human papillomavirus (HPV) persistent infections. HPV E7 oncoprotein is the major viral transforming factor, emerging as a natural candidate for immunotherapy, since it is constitutively expressed in HPV‐induced cancer cells. We have previously shown that E7 can self‐assemble into soluble and homogeneous spherical oligomers, named E7 soluble oligomers (E7SOs). These are highly resistant to thermal denaturation, providing an additional advantage given the demand for highly stable vaccine formulations. Here, we present a new chemically stabilized form of the E7SOs (E7SOx) and analyzed its effect in a murine HPV‐tumor model. Vaccination of female mice with low doses of E7SOx combined with a CpG‐rich oligonucleotide (ODN) as adjuvant elicits a strong long‐lasting protection against E7‐expressing tumor cells, preventing tumor outgrowth after rechallenge 90‐days later. Therapeutic experiments showed that E7SOx/ODN vaccination significantly delays tumor growth and extends the time of survival of the treated mice in a dose‐dependent manner. These proof‐of‐principle preclinical experiments denote the potential applicability of our E7SOx‐based vaccine to the treatment of cervical cancer and other mucosal HPV‐related neoplastic lesions. In addition to thermal, chemical and proteolysis stability, the combined recombinant and chemical modification nature of the E7SOx vaccine candidate, results in low‐cost, of particular interest in developing countries, where most of the cervical cancer cases occur and the most affected population is at reproductive age.
Prognostic significance of osteopontin in hepatocellular carcinoma: A meta‐analysis
Authors: Zhang Chuan‐Hai , Xu Ge‐Liang , Jia Wei‐Dong , Ge Yong‐Sheng , Li Jian‐Sheng , Ma Jin‐Liang , Ren Wei‐Hua 
Published: July 2011

AbstractOsteopontin (OPN) has been implicated in tumor development and progression for several years. However, the prognostic value of OPN overexpression in patients with hepatocellular carcinoma (HCC) remains controversial. We performed a meta‐analysis to assess the relationship between OPN overexpression and clinical outcome of HCC. A meta‐analysis of seven studies (1,158 patients) was carried out to evaluate the association between OPN and overall survival (OS) and disease‐free survival (DFS) in HCC patients. The correlation between OPN and tumor vascular invasion or other invasion‐related parameters was also assessed. Data were synthesized with random effect model of DerSimonian and Laird, hazard ratio (HR) or odds ratio (OR) with its 95% confidence interval (CI) was used as the effect size estimate. Our analysis results indicated that high OPN expression predicted poor OS (HR: 1.37, 95% CI: 1.21–1.55) and DFS (HR: 1.62, 95% CI: 1.24‐2.11) of HCC. OPN overexpression tended to be associated with the presence of tumor vascular invasion (OR: 1.93, 95% CI: 0.97–3.84) and advanced tumor grade (OR: 1.74, 95% CI: 0.95–3.18). By this study, we conclude that OPN overexpression indicates a poor prognosis for patients with HCC, it may also have predictive potential for HCC invasion and metastasis.
Novel insights in basic and applied stem cell therapy
Authors: Siniscalco Dario , Giordano Antonio , Galderisi Umberto 
Published: July 2011

AbstractThe achievement of novel findings in stem cell research were the subject of the meeting organized by Stem Cell Research Italy (SCR Italy) and by the International Society for Cellular Therapy‐Europe (ISCT). Stem cell therapy represents great promise for the future of molecular and regenerative medicine. The use of several types of stem cells is a real opportunity to provide a valid approach to curing several untreatable human diseases. Before it is suitable for clinical applications, stem cell biology needs to be investigated further and in greater detail. Basic stem cell research could provide exact knowledge regarding stem cell action mechanisms, and pre‐clinical research on stem cells on an in vivo model of disease provides scientific evidence for future human applications. Applied stem cell research is a promising new approach to handling several diseases. Along with tissue engineering, it offers a new and promising discipline that can help to manage human pathologies through stem cell therapy. All of these themes were discussed in this meeting, covering stem cell subtypes with their newest basic and applied research. J. Cell. Physiol. 227: 2283–2286, 2012. © 2011 Wiley Periodicals, Inc.
Enhanced delivery of mda‐7/IL‐24 using a serotype chimeric adenovirus (Ad.5/3) in combination with the apogossypol derivative BI‐97C1 (Sabutoclax) improves therapeutic efficacy in low CAR colorectal cancer cells
Authors: Azab Belal , Dash Rupesh , Das Swadesh K. , Bhutia Sujit K. , Shen Xue‐Ning , Quinn Bridget A. , Sarkar Siddik , Wang Xiang‐Yang , Hedvat Michael , Dmitriev Igor P. , Curiel David T. , Grant Steven , Dent Paul , Reed John C. , Pellecchia Maurizio , Sarkar Devanand , Fisher Paul B. 
Published: July 2011

AbstractAdenovirus (Ad)‐based gene therapy represents a potentially viable strategy for treating colorectal cancer. The infectivity of serotype 5 adenovirus (Ad.5), routinely used as a transgene delivery vector, is dependent on Coxsackie‐adenovirus receptors (CAR). CAR expression is downregulated in many cancers thus preventing optimum therapeutic efficiency of Ad.5‐based therapies. To overcome the low CAR problem, a serotype chimerism approach was used to generate a recombinant Ad (Ad.5/3) that is capable of infecting cancer cells via Ad.3 receptors in a CAR‐independent manner. We evaluated the improved transgene delivery and efficacy of Ad.5/3 recombinant virus expressing melanoma differentiation associated gene‐7/interleukin‐24 (mda‐7/IL‐24), an effective wide‐spectrum cancer‐selective therapeutic. In low CAR human colorectal cancer cells RKO, wild‐type Ad.5 virus expressing mda‐7/IL‐24 (Ad.5‐mda‐7) failed to infect efficiently resulting in lack of expression of MDA‐7/IL‐24 or induction of apoptosis. However, a recombinant Ad.5/3 virus expressing mda‐7/IL‐24 (Ad.5/3‐mda‐7) efficiently infected RKO cells resulting in higher MDA‐7/IL‐24 expression and inhibition of cell growth both in vitro and in nude mice xenograft models. Addition of the novel Bcl‐2 family pharmacological inhibitor Apogossypol derivative BI‐97C1 (Sabutoclax) significantly augmented the efficacy of Ad.5/3‐mda‐7. A combination regimen of suboptimal doses of Ad.5/3‐mda‐7 and BI‐97C1 profoundly enhanced cytotoxicity in RKO cells both in vitro and in vivo. Considering the fact that Ad.5‐mda‐7 has demonstrated significant objective responses in a Phase I clinical trial for advanced solid tumors, Ad.5/3‐mda‐7 alone or in combination with BI‐97C1 would be predicted to exert significantly improved therapeutic efficacy in colorectal cancer patients. J. Cell. Physiol. 227: 2145–2153, 2012. © 2011 Wiley Periodicals, Inc.
Characterization of a versatile rhizospheric organism from cucumber identified as Ochrobactrum haematophilum
Authors: Zhao Lei , Teng Songshan , Liu Yanping 
Published: July 2011

AbstractSeveral rhizobacteria play a vital role in promoting plant growth and protecting plants against fungal diseases and degrading pesticides in the environment. In this study, a bacterial strain, designated H10, was isolated from the rhizosphere at Laixi in Shandong Province, China, and was identified as Ochrobactrum haematophilum based on API 20 NE tests and 16S rRNA gene sequence analysis. The plant growth‐promoting characteristics of the strain were further characterized, and the results showed that strain H10 produces siderophore, indol‐3‐acetic (IAA) and solubilized phosphate but lacks 1‐aminocyclopropane‐1‐carboxylate (ACC) deaminase activity. Inoculation with the strain was found to significantly increase (p < 0.05) the growth of cucumber in pot experiments. Strain H10 was assessed in vitro for antagonism against several pathogenic fungi and showed high antifungal activity. The cell‐free culture filtrates, which had high extracellular chitinase, β;‐1,3‐glucanase and protease activities, could inhibit the growth of all pathogenic fungi tested, indicating that growth suppression was partly due to extracellular antifungal metabolites present in the culture filtrates. Changes in hyphal morphology were observed in phytopathogenic fungi after treatment with the culture filtrates. Additionally, strain H10 was able to degrade 80%, 85% and 58% of the pesticides chlorpyrifos, β;‐cypermethrin and imidacloprid, respectively, within 60 h in liquid culture. The inoculation of strain H10 into soil treated with 100 mg kg–1of the three pesticides accordingly resulted in a higher degradation rate than in noninoculated soils. These results highlight the potential of this bacterium for use as a biofertilizer and biopesticide and suggest that it may provide an alternative to the use of chemical fertilizers and pesticides in agriculture. Additionally, it may represent a bioremediation agent that can remove contaminating chemical pesticide residues from the environment. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Surveillance of single‐cell behavior in different subpopulations of Ralstonia pickettii AR1 during growth and polyhydroxybutyrate production phases by flow cytometry
Authors: Shakeri Shahryar , Roghanian Rasoul , Emtiazi Giti 
Published: July 2011

AbstractMost bacterial strains accumulate intracellular polyhydroxybutyrate (PHB) granules as an energy reservoir, in response to fluctuations in their microenvironment. Flow cytometry was applied for the analysis of single cells of Ralstonia pickettii AR1 in response to changes in the culture conditions. Two parameters, the PHB production‐related FL2 and side scatter (SSC) parameters, were used to monitor, distinguish and characterize different subpopulations in the growth and PHB production phases. A high SSC level was observed in the mid‐log exponential growth phase. When the nitrogen source reached a limiting level, the SSC started to decrease, in contrast to the intracellular PHB granules‐related FL2 parameter. The results show that ammonium limitation is a critical and important factor for the accumulation of reserve compounds. Four subpopulations were observed and distinguished upon entrance of the cells into the exponential growth phase. When the cells entered the late exponential growth or early stationary phase, two subpopulations had disappeared and only two, different subpopulations were dominant. One of the subpopulations with changed SSC and PHB production activity switched to another subpopulation that was only active in PHB production in the stationary phase. The whole cells of R. pickettii AR1 tended to form a homogeneous population at the end of the stationary phase. In fact, the changes in the subpopulations of a single strain are related to different physiological states of the cells. The observation of different subpopulations suggests that each subpopulation shows a specific response to changes in the surrounding microenvironment, nutrients and limiting factors. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
GC content‐independent amino acid patterns in Bacteria and Archaea
Authors: Schmidt Andre , Rzanny Michael , Schmidt Astrid , Hagen Matthias , Schütze Eileen , Kothe Erika 
Published: July 2011

AbstractEvery organism can be characterized by the amino acid composition of its proteome. So far it was assumed that these compositions are determined by the GC content of the DNA or, in some cases, by extreme lifestyles, like thermophily or halophily. Here, we focussed our analysis on eight amino acids, each of which is encoded by both, GC and AT rich codons, to identify finer amino acid patterns beyond the GC dominance. We investigated the conceptually translated proteomes of 1029 bacterial and archaeal strains with sequenced genomes for amino acid composition. Using correspondence analysis, we found that phylogenetic groups within bacteria and archaea generally can be discriminated from other groups due to their amino acid composition. In some cases, single organisms, e.g. Treponema pallidum strains or Mycoplasma penetrans, are characterized by extreme amino acid compositions. We assume that our data could provide a basis for a new approach to analyze evolution of bacterial and archaeal groups. Furthermore, for single organisms, the detailed knowledge of the amino acid composition of the entire proteome encoded in the genome could lead to a better understanding, important for pharmaceutical or biotechnological applications. We recommend that information about amino acid compositions should be provided in databases, comparable to the GC content of genomes. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
40‐O ‐[2‐Hydroxyethyl]rapamycin modulates human dendritic cell function during exposure to Aspergillus fumigatus
Authors: Bauer Ruth , Mezger Markus , Blockhaus Christian , Schmitt Anna‐Lena , Kurzai Oliver , Einsele Hermann , Loeffler Juergen 
Published: July 2011

Abstract40‐O ‐[2‐Hydroxyethyl]rapamycin (RAD), a novel derivative of the immunosuppressive drug rapamycin, was analyzed for its immunomodulatory influence during the interaction of human monocyte‐derived dendritic cells (moDC) with Aspergillus fumigatus. RAD is clinically used to prevent graft‐versus ‐host disease as well as solid organ and bone marrow transplant rejection. However, it may constitute a risk factor for the development of opportunistic infections, such as invasive aspergillosis which is mainly caused by the most common airborne fungal pathogen A. fumigatus. moDC were generated in the presence or absence of RAD. In this setting, RAD had various modulating effects on the immune function of DC. A decrease of pro‐ and anti‐inflammatory cytokines (IL‐12, TNF‐α, CCL20, IL‐10) was observed. Furthermore, RAD reduced the expression of innate immunity receptors (TLR2, TLR4, dectin‐1), impaired the maturation capacity of moDC observed through the reduction of costimulatory factors (CD40, CD80, CD83, CD86), and impaired their capacity to phagocytose and damage A. fumigatus. These data demonstrate that RAD influences the differentiation of DC. RAD modulates the cytokine response of DC to A. fumigatus and reduces their ability to kill germ tubes. Thus, RAD treatment might affect the risk of invasive aspergillosis independently of its capacity of blocking T cell activation. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Isolation and characterization of Klebsiella oxytoca strain degrading crude oil from a Tunisian off‐shore oil field
Authors: Chamkha Mohamed , Trabelsi Yosra , Mnif Sami , Sayadi Sami 
Published: July 2011

AbstractA facultatively anaerobic, Gram‐negative, mesophilic, moderately halotolerant, non‐motile, and non‐sporulated bacterium, designated strain BSC5 was isolated from an off‐shore “Sercina” oil field, located near the Kerkennah island, Tunisia. Yeast extract was not required for growth. Phenotypic characteristics and phylogenetic analysis of the 16S rRNA gene sequence of strain BSC5 revealed that it was related to members of the genus Klebsiella, being most closely related to the type strain of K. oxytoca (99% sequence similarity). Strain BSC5 was capable of using aerobically the crude oil as substrate growth. The growth of strain BSC5 on crude oil was followed by measuring the OD600 nm and by enumeration of viable cells at different culture's time. GC‐MS analysis showed that strain BSC5 was capable of degrading a wide range of aliphatic hydrocarbons from C13 to C30. The biodegradation rate for n ‐alkanes reached 44% and 75%, after 20 and 45 days of incubation, respectively. Addition of the synthetic surfactant, Tween 80, accelerated the crude oil degradation. The biodegradation rate for n ‐alkanes reached 61% and 98%, after 20 and 45 days of incubation, respectively. Moreover, three aromatic compounds, p ‐hydroxybenzoate, protocatechuate and gentisate, were metabolized completely by strain BSC5 after 24 h, under aerobic conditions. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Development of Loop‐mediated isothermal amplification method for rapid detection of Streptococcus iniae, the causative agent of streptococcicosis in fish
Authors: Cai Shuang‐Hu , Wang Bei , Lu Yi‐Shan , Jian Ji‐Chang , Wu Zao‐He 
Published: July 2011

AbstractStreptococcus iniae is a major pathogen that causes sever economic losses in tilapia aquaculture. A set of four specific primers was designed by targeting lctO gene. With Bst DNA polymerase, the target DNA can be clearly amplified for 60 min at 64 °C in a simple water bath. The sensitivity of the LAMP assay for the detection of S. iniae is about 12.4 cells per reaction in both of pure cultures and added fish tissues cultures. LAMP products could be judged with agar gel or naked eye after addition of SYBR Green I. There were no cross‐reactions with other bacterial strains indicating high specificity of the LAMP. The LAMP method was also applied to detect S. iniae ‐infected tilapia tissues effectively. The LAMP assay reported here indicates the potential usefulness of the technique as a valuable simple, rapid alternative procedure for the detection of S. iniae during streptococcicosis monitoring of cultured fish. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Identification and biocontrol efficacy of Streptomyces miharaensis producing filipin III against Fusarium wilt
Authors: Kim Jeong Do , Han Jae Woo , Hwang In Cheon , Lee Dongho , Kim Beom Seok 
Published: July 2011

AbstractA number of bacterial strains were isolated from the internal tissue of Trapa japonica. Of these, strain KPE62302H, which had a 16S rDNA sequence identical to that of Streptomyces miharaensis showed antifungal activity against several plant pathogens. Treatment of seeds with strain KPE62302H induced a significant reduction in the incidence of Fusarium wilt in tomato plants compared with untreated controls. An antifungal substance (FP‐1) was purified from the culture extract of strain KPE62302H using C18 flash and Sephadex LH‐20 column chromatography and reverse phase HPLC. Extensive spectrometric analysis using MS and NMR identified this as filipin III. FP‐1 inhibited the mycelial growth of plant pathogenic fungi such as Alternaria mali, Aspergillus niger, Colletotrichum gloeosporioides, C. orbiculare, Cylindrocarpon destructans, Diaporthe citiri, Fusarium oxysporum at 1–10 μg ml–1 and also markedly inhibited the development of Fusarium wilt caused by F. oxysporum f.sp. lycopersici in tomato plants by treatment with 10 μg ml–1 under greenhouse conditions. The efficacy of FP‐1 against Fusarium wilt was comparable to that of the synthetic fungicide benomyl. An egfp ‐tagged strain of KPE62302H confirmed its ability to colonize tomato plants. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Multidrug resistance reversal effect of DMC derived from buds of Cleistocalyx operculatus in human hepatocellular tumor xenograft model
Authors: Huang Hai‐ya , Niu Jiang‐long , Lu Yan‐hua 
Published: July 2011

AbstractBACKGROUND: Multidrug resistance (MDR) is a major obstacle in the chemotherapeutic treatment of many human cancers. 2′,4′‐Dihydroxy‐6′‐methoxy‐3′,5′‐dimethylchalcone (DMC) isolated from the buds of Cleistocalyx operculatus (Roxb.) Merr. et Perry (Myrtaceae), was investigated for its reversal effects on cancer cell MDR.RESULTS: A human hepatocellular tumor xenograft model was established with the BEL‐7402/5‐FU cell line. Combined 5‐fluorouracil (5‐FU) and DMC (40 mg kg−1) treatment significantly elevated tumor inhibition rate to 72.2%. DMC could also increase 5‐FU concentrations in tumor tissues and increase caspase‐3 activity. Also, combined therapy resulted in enhanced tumor apoptotic and reduced proliferative activities relative to 5‐FU alone. Examining body weight and other signs of unwanted toxicity of the different treatment groups revealed no significant signs of adverse effects.CONCLUSION: All results suggested that DMC reverses 5‐FU resistance, with a benign side effects profile. Copyright © 2011 Society of Chemical Industry
Polyunsaturated fatty acid levels in rat tissues after chronic treatment with dietetic oils
Authors: Rustichelli Cecilia , Avallone Rossella , Campioli Enrico , Braghiroli Daniela , Baraldi Mario 
Published: July 2011

AbstractBACKGROUND: The essential fatty acids can be helpful in the prevention of several pathologies. The purpose of this study was to quantify the major n‐3 and n‐6 fatty acids in tissues of rats fed with flaxseed oil and with a dietetic oil in order to evaluate how their chronic supplementation could influence the correspondent in vivo levels and to study the effectiveness of the dietetic oil compared to flaxseed oil.RESULTS: Fatty acids were successfully extracted from biological samples, subjected to derivatization procedure and analysed by high‐performance liquid chromatography with UV detection under gradient elution mode. The developed method showed good linearity, precision and accuracy, with recoveries ranging from 89% to 92%. Animals treated with flaxseed and dietetic oils showed enhanced levels of n‐3 fatty acids compared to control groups, with significantly higher levels of eicosapentaenoic acid and docosahexaenoic acid in the brain and in the adipose tissue of the dietetic group compared to the flaxseed group.CONCLUSION: The obtained data underline that the tested oils can effectively enhance the tissue levels of n‐3 fatty acids and therefore they could be successfully used in the dietetic treatment of lipid‐related diseases. Copyright © 2011 Society of Chemical Industry
Affinity capillary electrophoresis and quantum mechanical calculations applied to the investigation of hexaarylbenzene‐based receptor binding with lithium ion
Authors: Ehala Sille , Toman Petr , Rathore Rajendra , Makrlík Emanuel , Kašička Václav 
Published: July 2011

AbstractIn this study, two complementary approaches, affinity capillary electrophoresis (ACE) and quantum mechanical density functional theory (DFT) calculations, have been employed for quantitative characterization and structure elucidation of the complex between hexaarylbenzene (HAB)‐based receptor R and lithium ion Li+. First, by means of ACE, the apparent binding constant of LiR+ complex (K) in methanol was determined from the dependence of the effective electrophoretic mobilities of LiR+ complex on the concentration of lithium ions in the 25 mM Tris/50 mM chloroacetate background electrolyte (BGE) using non‐linear regression analysis. Prior to regression analysis, the effective electrophoretic mobilities of the LiR+ complex were corrected to reference temperature 25°C and constant ionic strength 25 mM. The apparent binding constant of the LiR+ complex in the above methanolic BGE was evaluated as logK = 1.15±0.09. Second, the most probable structures of nonhydrated LiR+ and hydrated LiR+·3H2O complexes were derived by DFT calculations. The optimized structure of the hydrated LiR+·3H2O complex was found to be more realistic than the nonhydrated LiR+ complex because of the considerably higher binding energy of LiR+·3H2O complex (500.4 kJ/mol) as compared with LiR+ complex (427.5 kJ/mol).