bluuebunny/crossref_metadata_2025_split huggingface dataset:
S100a9 might act as a modulator of the Toll-like receptor 4 transduction pathway in chronic rhinosinusitis with nasal polyps
Authors: Khayer Nasibeh , Jalessi Maryam , Farhadi Mohammad , Azad Zahra 
Published: April 2024

AbstractChronic rhinosinusitis with nasal polyp (CRSwNP) is a highly prevalent disorder characterized by persistent nasal and sinus mucosa inflammation. Despite significant morbidity and decreased quality of life, there are limited effective treatment options for such a disease. Therefore, identifying causal genes and dysregulated pathways paves the way for novel therapeutic interventions. In the current study, a three-way interaction approach was used to detect dynamic co-expression interactions involved in CRSwNP. In this approach, the internal evolution of the co-expression relation between a pair of genes (X, Y) was captured under a change in the expression profile of a third gene (Z), named the switch gene. Subsequently, the biological relevancy of the statistically significant triplets was confirmed using both gene set enrichment analysis and gene regulatory network reconstruction. Finally, the importance of identified switch genes was confirmed using a random forest model. The results suggested four dysregulated pathways in CRSwNP, including “positive regulation of intracellular signal transduction”, “arachidonic acid metabolic process”, “spermatogenesis” and “negative regulation of cellular protein metabolic process”. Additionally, the S100a9 as a switch gene together with the gene pair {Cd14, Tpd52l1} form a biologically relevant triplet. More specifically, we suggested that S100a9 might act as a potential upstream modulator in toll-like receptor 4 transduction pathway in the major CRSwNP pathologies.
Mechanical shock test simulation analysis of butterfly valves developed for the naval defense industry and evaluation of real test and production data
Authors: Ozkan Erhan 
Published: April 2024

AbstractThe main purpose of this study is to investigate the mechanical shock behavior and develop the shock resistance of widely preferred butterfly valves for navy defense industries by handling the real test results with computer aided design and simulation programs. The 2D and 3D drawings were realized by using solid modeling and design programs. Mechanical analyzes to determine the mechanical strength of the specimens were carried out with the finite element analysis method by using structural simulation program. Mechanical shock test simulations were carried out by with shock response spectrum analysis. Solidification, filling-time–temperature analyzes, and simulation studies of inner stresses caused by micro and macro shrinkages were performed by using the casting simulation program. Comparisons of virtual tests simulated in computer environment with real tests were done in shock test setup. Products made of bronze were preferred due to the high corrosion resistance and the desire to be a useful research article that can respond to common applications in the defense industry. Virtual shock test simulation and real shock tests were performed according to the MIL STD 810 standard. The shock test results observations showed that by revising the design with a safety factor of 18% on the specimen, it was ensured that the product could pass the mechanical shock test even at an acceleration of 4000 m/s2. Then material become safe to use. With the use of a three-way feeder in the production of the reinforced design the difference in net weight from 19% has been reduced to 12%, while the production time has been improved by 22%.
Development and preliminary validation of a prediction formula of sodium and sodium-to-potassium ratio based on multiple regression using 24-h urines
Authors: Yamagishi Marina , Takachi Ribeka , Ishihara Junko , Maruya Sachiko , Ishii Yuri , Kito Kumiko , Nakamura Kazutoshi , Tanaka Junta , Yamaji Taiki , Iso Hiroyasu , Iwasaki Motoki , Tsugane Shoichiro ,   The JPHC-NEXT Protocol Validation Study Group, Tsugane S. , Iwasaki M. , Sawada N. , Yamaji T. , Ishii Y. , Iso H. , Ishihara J. , Nakamura K. , Tanaka J. , Takachi R. , Inoue M. , Sasazuki S. , Shimazu T. , Charvat H. , Noda A. , Hara A. , Mishiro I. , Shinozawa Y. , Umezawa J. , Takahashi T. , Ito Y. , Kobayashi K. , Kitamura K. , Sawada Norie 
Published: April 2024

AbstractAccurate measurement of sodium intake in the diet is challenging, and epidemiological studies can be hampered by the attenuation of associations due to measurement error in sodium intake. A prediction formula for habitual 24-h urine sodium excretion and sodium-to-potassium ratio might lead to more reliable conclusions. Five 24-h urinary samples and two Food Frequency Questionnaires (FFQs) were conducted among 244 Japanese participants aged 35–80 years. We conducted multivariate linear regression analysis with urinary excretion as dependent variables and eating behaviour and food frequency as independent variables. Empirical weights of sodium excretion and sodium-to-potassium ratio were extracted. Preliminary validity was also assessed by randomly dividing the subjects into development and validation groups based on the correlation coefficient between estimates by the prediction formula and urinary excretion. Taste preference, soy sauce use at the table, frequency of pickled vegetables intake and number of bowls of miso soup were extracted as determinants of sodium excretion. Correlation coefficients between the estimates and urinary excretion for men and women were 0.42 and 0.43, respectively, for sodium and 0.49 and 0.50, respectively, for sodium-to-potassium ratio. This prediction formula may provide more accurate estimation of sodium intake and sodium-to-potassium ratio than the food composition approach.
Differences in cardiovascular risk and health-related quality of life in COPD patients according to clinical phenotype
Authors: Montiel Ana Muñoz , Ruiz-Esteban Pedro , Del Río Adolfo Doménech , Valdivielso Pedro , Chaparro Miguel Ángel Sánchez , Olveira Casilda 
Published: April 2024

AbstractChronic obstructive pulmonary disease (COPD) has a high prevalence and a major impact on health-related quality of life (HRQL). COPD exacerbations are an important cause of morbidity and mortality, affecting cardiovascular risk, and are associated with poorer health status. The aim of this study was to assess the association between cardiovascular risk (CVR) and HRQL, according to exacerbator or non-exacerbator phenotype. We undertook a cross-sectional, observational, descriptive study of 107 patients with COPD. Patients with two or more moderate exacerbations or one severe exacerbation in the previous year were considered as exacerbators. The CVR was calculated with the Framingham scale and SCORE (Systematic Coronary Risk Evaluation) and the HRQL was assessed with the generic questionnaire Short Form-36 Health Survey (SF-36), the St George Respiratory Questionnaire (SGRQ) and the COPD Assessment Test (CAT). Statistical analysis was done with SPSS version 26.0 for Windows. The SF-36 and the SGRQ showed lower values for the exacerbator phenotype, indicating a poorer quality of life. The CAT questionnaire showed values above 10 for the exacerbator phenotype, and lower values in the non-exacerbator group. After categorizing the sample according to their median age (65 years), we found a greater deterioration in HRQL in patients under 65 years of age according to the SF-36, the SGRQ and the CAT. We also detected differences in HRQL between non-exacerbator patients with a high CVR according to the Framingham (≥ 20%) and SCORE (≥ 5%) scales compared to those without this risk. A tendency towards worse HRQL was observed in non-exacerbator patients with a high CVR, which was statistically significant for the SGRQ impact domain on the SCORE scale. The CAT also showed a worse quality of life in non-exacerbator patients with a high CVR, which was significant in the Framingham model (Framingham high risk 8.41 vs non-high risk 6.05, p < 0.01). These differences were not observed in exacerbator patients. Our findings confirm that a high CVR influences HRQL in patients with COPD, especially in non-exacerbator patients with a high CVR, measured according to the SGRQ and the CAT.
Oral microbial signatures associated with age and frailty in Canadian adults
Authors: DeClercq Vanessa , Wright Robyn J. , Nearing Jacob T. , Langille Morgan G. I. 
Published: April 2024

AbstractThis study aimed to assess the association between the oral microbiome, age, and frailty. Data and saliva samples were obtained from male and female participants aged 35–70 years (n = 1357). Saliva samples were analysed by 16S rRNA gene sequencing and differences in microbial diversity and community compositions were examined in relation to chronological age and the frailty index (FI). Most alpha diversity measures (Richness, Shannon Diversity, Faith’s Phylogenetic Diversity) showed an inverse association with frailty, whereas a positive association was observed with age and Shannon Diversity and Evenness. A further sex-stratified analysis revealed differences in measures of microbial diversity and composition. Multiple genera were detected as significantly differentially abundant with increasing frailty and age by at least two methods. With age, the relative abundance of Veillonella was reduced in both males and females, whereas increases in Corynebacterium appeared specific to males and Aggregatibacter, Fusobacterium, Neisseria, Stomatobaculum, and Porphyromonas specific to females. Beta diversity was significantly associated with multiple mental health components of the FI. This study shows age and frailty are differentially associated with measures of microbial diversity and composition, suggesting the oral microbiome may be a useful indicator of increased risk of frailty or a potential target for improving health in ageing adults.
Entropy optimization of lid-driven micropolar hybrid nanofluid flow in a partially porous hexagonal-shaped cavity with relevance to energy efficient storage processes
Authors: Ahlawat Anil , Chaudhary Shilpa , Sharma Mukesh Kumar , Loganathan K. , Pattanaik Balachandra , Balaram Allam 
Published: April 2024

AbstractHydromagnetically associated heat convection can greatly enhance the performance of high-efficiency thermal appliances and renewable energy sources through an optimized design. This investigation examines the production of thermodynamic irreversibility and heat convection for a double lid-driven flow within a partially porous stratified hexagonal enclosure. The top and bottom-wall are moving in the opposite direction with an equal velocity U0. The top-wall and the bottom-wall are kept at temperature Tc and Th (Th $$>$$
>
Tc) while the slanted walls are assumed to be thermally insulated. A constant magnetic field is employed in the horizontal x-direction. The hexagonal cavity was filled with a micropolar hybrid nanofluid Ag-MgO/water. The system of dimensionless equations was solved by the finite difference method (FDM) associated with successive over-relaxation (SOR), successive under-relaxation (SUR), and Gauss–Seidel iteration tactics and required results are computed with problem specific program in MATLAB code. The results indicate that the Ra and the thickness of the porous layer (Xp) significantly influences heat convection and thermal irreversibility processes. The Nuavg and STotal rises 6.299% and 3.373% as ‘$${\upphi }_{{\text{hnf}}}$$

ϕ
hnf

’ enhances from 0 to 4%, respectively. Furthermore, as the values of Ra, Ha, K0, and $${\upphi }_{{\text{hnf}}}$$

ϕ
hnf

increase, Beavg experiences a decline of 53.73%, 11.04%, 38.36%, and 0.09% respectively. Also, movement of wall has a significant impact on heat transfer rates and entropy production. The present study may be extended in numerous areas to mimic the problems like—(1) onset of thermo-mechanical process for solid–fluid interaction in a conduit. (2) Thermos-chemical process with extraction of ions in two-phase fluid for double layer plating on a continuously moving sheet, as region of porous stratum saturated with a class of fluid and region without porous medium occupied with other fluid.
Effect of wear on the dynamic characteristics of a rigid rotor supported by journal bearings
Authors: Raja Moorthi Logamurthi , Inayat-Hussain Jawaid Iqbal , Abidin Zakaria Azrul 
Published: April 2024

AbstractThe purpose of this work is to examine the effects of wear severity on the static and dynamic characteristics of journal bearings, and on the vibration response of a rigid rotor supported by journal bearings. Numerical simulations using MATLAB was conducted for three different operating regimes, namely low loaded operating regime ($$\epsilon _0 = 0.15$$

ϵ
0

=
0.15

), moderately loaded operating regime ($$\epsilon _0 = 0.45$$

ϵ
0

=
0.45

) and highly loaded operating regime ($$\epsilon _0 = 0.75$$

ϵ
0

=
0.75

) with wear depth parameter ratio ($$\delta$$
δ
) varied from 0 to 0.5 at increments of 0.1. Numerical results showed that the vibration response of the rotor generally increases with the increase of the wear depth for all cases of low, moderately and highly loaded operating regimes of the bearings. For the values of parameters considered in this work, it was shown that the vibration response amplitude of the rotor in worn journal bearings may be six times larger compared to the response amplitude of the rotor in non-worn bearings.
Characterization of macrophages in head and neck squamous cell carcinoma and development of MRG-based risk signature
Authors: Liu Lei , Liu Qiang 
Published: April 2024

AbstractMacrophages are immune cells in the TME that can not only inhibit angiogenesis, extracellular matrix remodeling, cancer cell proliferation, and metastasis but also mediate the phagocytosis and killing of cancer cells after activation, making them key targets in anti-tumor immunotherapy. However, there is little research on macrophages and their relation to disease prognosis in HNSCC. Initially, we collected scRNA-seq, bulk RNA-seq, and clinical data. Subsequently, we identified macrophages and distinguished MRGs. Using the K-means algorithm, we performed consensus unsupervised clustering. Next, we used ssGSEA analysis to assess immune cell infiltration in MRG clusters. A risk model was established using multivariate Cox analysis. Then, Kaplan–Meier, ROC curves, univariate and multivariate COX analyses, and C-index was used to validate the predictive power of the signature. The TIDE method was applied to assess the response to immunotherapy in patients diagnosed with HNSCC. In addition, drug susceptibility predictions were made for the GDSC database using the calcPhenotype function. We found that 8 MRGs had prognostic potential. Patients in the MRG group A had a higher probability of survival, and MRG clusters A and B had different characteristics. Cluster A had a higher degree of expression and infiltration in MRG, indicating a closer relationship with MRG. The accuracy of the signature was validated using univariate and multivariate Cox analysis, C-index, and nomogram. Immune landscape analysis found that various immune functions were highly expressed in the low-risk group, indicating an improved response to immunotherapy. Finally, drugs with high sensitivity to HNSCC (such as 5-Fluorouracil, Temozolomide, Carmustine, and EPZ5676) were explored and analyze the malignant characteristics of HNSCC. We constructed a prognostic model using multivariate Cox analysis, consisting of 8 MRGs (TGM2, STC1, SH2D3C, PIK3R3, MAP3K8, ITGA5, ARHGAP4, and AQP1). Patients in the low-risk group may have a higher response to immunotherapy. The more prominent drugs for drug selection are 5-fluorouracil, temozolomide and so on. Malignant features associated with HNSCC include angiogenesis, EMT, and the cell cycle. This study has opened up new prospects for the prognosis, prediction, and clinical treatment strategy of HNSCC.
St. John's wort extract Ze 117 alters the membrane fluidity of C6 glioma cells by influencing cellular cholesterol metabolism
Authors: Bremer Swen , Weitkemper Eva , Häberlein Hanns , Franken Sebastian 
Published: April 2024

AbstractChronic stress is associated with major depressive disorder (MDD). Increased glucocorticoid levels caused by uncontrolled release through the hypothalamic‒pituitary‒adrenal (HPA) axis can cause changes in the lipid content of the cellular plasma membrane. These changes are suspected to be involved in the development of depressive disorders. St. John's wort extract (SJW) Ze 117 has long been used as an alternative to synthetic antidepressants. Part of its effect may be due to an effect on the cellular lipid composition and thus on the properties of plasma membranes and receptor systems embedded therein. In this study, we investigated the effect of Ze 117 on that of dexamethasone and simvastatin. Dexamethasone increases the fluidity of C6 cell plasma membranes. This effect is counteracted by administration of Ze 117. Here we demonstrate that this is not due to a change in C16:1/16:0 and C18:1/18:0 ratios in C6 cell fatty acids. On the other hand, Ze 117 increased the cellular cholesterol content by 42.5%, whereas dexamethasone reduced cholesterol levels similarly to simvastatin. Lowering cholesterol levels by dexamethasone or simvastatin resulted in decreased β-arrestin 2 recruitment to the 5-HT1a receptor. This effect was counterbalanced by Ze 117, whereas the SJW extract had little effect on β-arrestin 2 recruitment in non-stressed cells. Taken together, in C6 cells, Ze 117 induces changes in membrane fluidity through its effect on cellular cholesterol metabolism rather than by affecting fatty acid saturation. This effect is reflected in an altered signal transduction of the 5-HT1a receptor under Ze 117 administration. The current in vitro results support the hypothesis that Ze 117 addresses relevant parts of the cellular lipid metabolism, possibly explaining some of the antidepressant actions of Ze 117.
Perlite incorporation for sedimentation reduction and improved properties of high-density geopolymer cement for oil well cementing
Authors: Abdelaal Ahmed , Elkatatny Salaheldin , Abdel Fattah Ahmed Mohsen 
Published: April 2024

AbstractPortland cement (PC) is known for its environmental and technical concerns and massive energy consumption during manufacturing. Geopolymer cement is a promising technology to totally replace the use of PC in the oil and gas industry. Although geopolymers are widely used in the construction industry, it is yet to see a full-scale application in the petroleum industry. High-density geopolymer cement development is essential to substitute heavy-weight Portland cement slurries for high pressure well cementing applications. Sedimentation issue is associated with high-density cement slurries which use high specific gravity solids such as weighting materials. This problem causes heterogeneity and density variation along the cemented sections. The main target of this work is to evaluate the use of perlite powder to address the sedimentation issue in the heavy weight geopolymer systems. Hematite-based Class F fly ash (FFA) geopolymer cement slurries with perlite concentrations of 0, 1.5, and 3% by weight of binder (BWOB) were prepared. The sedimentation problem was investigated using three techniques: API method, nuclear magnetic resonance (NMR), and computed tomography (CT) scan. The perlite effects on different geopolymer properties such as unconfined compressive strength (UCS), porosity, elastic and rheological properties were assessed. The results proved that perlite incorporation in high-density hematite-based FFA geopolymer significantly reduced sedimentation issue by increasing yield point and gel strength. NMR and CT scan showed that perlite decreases porosity and density variation across the geopolymer samples. The UCS increased with increasing perlite percentage from 0 to 3%. The measured Young’s moduli (YM) and Poisson’s ratios (PR) showed that the developed perlite based geopolymer systems are considered more flexible than Class G cement systems. It was found that the optimum perlite concentration is 3% BWOB for tackling sedimentation and developing a slurry with acceptable mixability and rheological properties.
A decision tree algorithm to identify predictors of post-stroke complex regional pain syndrome
Authors: Katsura Yuichi , Ohga Satoshi , Shimo Kazuhiro , Hattori Takafumi , Yamada Tsukasa , Matsubara Takako 
Published: April 2024

AbstractThis prospective cohort study aimed to identify the risk factors for post-stroke complex regional pain syndrome (CRPS) using a decision tree algorithm while comprehensively assessing upper limb and lower limb disuse and physical inactivity. Upper limb disuse (Fugl-Meyer assessment of upper extremity [FMA-UE], Action Research Arm Test, Motor Activity Log), lower limb disuse (Fugl-Meyer Assessment of lower extremity [FMA-LE]), balance performance (Berg balance scale), and physical inactivity time (International Physical Activity Questionnaire-Short Form [IPAQ-SF]) of 195 stroke patients who visited the Kishiwada Rehabilitation Hospital were assessed at admission. The incidence of post-stroke CRPS was 15.4% in all stroke patients 3 months after admission. The IPAQ, FMA-UE, and FMA-LE were extracted as risk factors for post-stroke CRPS. According to the decision tree algorithm, the incidence of post-stroke CRPS was 1.5% in patients with a short physical inactivity time (IPAQ-SF < 635), while it increased to 84.6% in patients with a long inactivity time (IPAQ-SF ≥ 635) and severe disuse of upper and lower limbs (FMA-UE score < 19.5; FMA-LE score < 16.5). The incidence of post-stroke CRPS may increase with lower-limb disuse and physical inactivity, in addition to upper-limb disuse. Increasing physical activity and addressing lower- and upper-limb motor paralysis may reduce post-stroke CRPS.
Vesicle protrusion induced by antimicrobial peptides suggests common carpet mechanism for short antimicrobial peptides
Authors: Park Peter , Matsubara Danilo K. , Barzotto Domenico R. , Lima Filipe S. , Chaimovich Hernan , Marrink Siewert J. , Cuccovia Iolanda M. 
Published: April 2024

AbstractShort-cationic alpha-helical antimicrobial peptides (SCHAMPs) are promising candidates to combat the growing global threat of antimicrobial resistance. They are short-sequenced, selective against bacteria, and have rapid action by destroying membranes. A full understanding of their mechanism of action will provide key information to design more potent and selective SCHAMPs. Molecular Dynamics (MD) simulations are invaluable tools that provide detailed insights into the peptide-membrane interaction at the atomic- and meso-scale level. We use atomistic and coarse-grained MD to look into the exact steps that four promising SCHAMPs—BP100, Decoralin, Neurokinin-1, and Temporin L—take when they interact with membranes. Following experimental set-ups, we explored the effects of SCHAMPs on anionic membranes and vesicles at multiple peptide concentrations. Our results showed all four peptides shared similar binding steps, initially binding to the membrane through electrostatic interactions and then flipping on their axes, dehydrating, and inserting their hydrophobic moieties into the membrane core. At higher concentrations, fully alpha-helical peptides induced membrane budding and protrusions. Our results suggest the carpet mode of action is fit for the description of SCHAMPs lysis activity and discuss the importance of large hydrophobic residues in SCHAMPs design and activity.