bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of bunazosin on monocrotaline‐induced pulmonary hypertension in rats
Authors: INOUE MAKOTO , WATANABE KOJI , MORI CHUZO , TANAKA OSAMU 
Published: January 2011

AbstractIn order to estimate the long‐term hemodynamic and histologic effects of bunazosin hydrochloride on primary pulmonary hypertension (PPH), for which effective internal therapy has not yet been proposed, we produced subacute pulmonary hypertension in 6 week old Sprague‐Dawley rats by injecting one dose of monocrotaline. Bunazosin (10 mg/kg) was administered intraperitoneally every day and bodyweight and hemodynamic parameters were measured. Right ventricle (RV) and left ventricle with septum (LV + S) were weighed separately. Medial thickness of the small pulmonary artery was calculated.In the bunazosin group, the increase of right ventricular systolic pressure (RVSP) as pulmonary arterial systolic pressure and the ratio of RVSP to pulmonary flow were limited, without causing systemic hypotension, compared to the control group. Weight ratio of RV/(LV + S) had a tendency to be limited in the bunazosin group. As for medial thickness, there was no significant difference between the two groups.It may be concluded that bunazosin hydrochloride should be considered for clinical trials in treatment of PPH in humans.
Specific IgE antibodies and serum eosinophil cationic protein in children with atopic dermatitis alone
Authors: URISU ATSUO , KONDO YASUTO , WADA EIKO , HORIBA FUMIYA , TSURUTA MITSUTOSI , YASAKI TAKEHIKO 
Published: January 2011

AbstractBoth eosinophils and specific immunoglobulin E (IgE) to foods and mites have been considered involved in the pathogenesis of atopic dermatitis (AD). The relationship between eosinophils and specific IgE, however, remains to be elucidated.Blood eosinophil counts, serum eosinophil cationic protein (ECP) and IgE to egg white, cow's milk, soybean, rice and Dermatophagoides pteronyssinus (Dp) were measured in subjects with AD alone or bronchial asthma (BA) alone. Subjects with positive IgE titers (Pharmacia radioallergosorbent test (RAST) units > 0.7) of one or more items were defined as RAST‐positive.Immunoglobulin E titers to egg white, cow's milk and soybean of subjects with AD were high in early childhood and declined with aging, whereas the titers of subjects with BA were negative or low. Immunoglobulin E titers to Dp were elevated after 1 year of age in both disease groups. Eosinophil cationic protein (ECP) levels and blood eosinophil counts in the AD and BA groups were significantly higher than those of non‐atopic controls. No difference in ECP levels or blood eosinophil counts were observed between RAST‐positive and negative groups.It is concluded that IgE to foods such as egg white, cow's milk and soybean might have a role in the pathogenesis of AD of young children, while IgE to mites might be involved in older children. Eosinophils may also participate in AD. However, different mechanisms may be responsible for the rise in specific IgE and high ECP levels and blood eosinophil counts.
A study of the iron requirement in infants, using changes in total body iron determined by hemoglobin, serum ferritin and bodyweight
Authors: HOKAMA TOMIKO 
Published: January 2011

AbstractThe amount of iron absorption in healthy breast‐fed infants was estimated in order to assess the iron requirement. This was carried out by monitoring changes in total body iron (TBI). Hemoglobin and serum ferritin were measure to calculate hemoglobin iron, body storage iron and TB1 at the ages of 4–5 months and 10–11 months. The amount of iron absorption was ∼ 0.4 mg/day, calculated as the sum of TBI increment and physiological iron loss. From this study, the dietary iron requirement for the weaning period was estimated as 4 mg/day.
Factors affecting the efficiency of peripheral blood stem cell collection in children treated with chemotherapy and G‐CSF
Authors: URASHIMA MITSUYOSHI , KATO YOKO , HOSHI YASUTAKA , DEGUCHI YASUSHI , KAMIJO MAKOTO , UCHIYAMA HIROSHI , FUJISAWA KOHJI , AKATSUKA JUN‐ICHI , MAEKAWA KIHEI 
Published: January 2011

AbstractThis retrospective study attempts to clarify the optimal timing for peripheral blood stem cell (PBSC) collection after conventional chemotherapy followed by granulocyte‐colony stimulating factor (G‐CSF) administration. Leukapheresis was performed 32 times in nine children with various cancers during bone marrow recovery phase following transient pancytopenia after chemotherapy. (On two occasions, leukapheresis was excluded because many leukemic blasts were included.) When the number of white blood cells (WBC) exceeded 1.8 times 1010/L after administration of G‐CSF (200 μg/m2, continuous infusion), many more CD34+ cells were contained in the collected peripheral mononuclear cells (P > 0.02) and a sufficient number of PBSC for transplantation (≥ 10 times 108 CD34+ cells/kg) was obtained after one run in 15 of 17 leukapheresis sessions. In contrast, sufficient PBSC were obtained only in one of 13 runs of leukapheresis when the number of WBC was < 1.8 times 1010/L. The number of WBC on the day when PBSC were collected correlated with collected nuclear cell number (r = 0.60), but not with the CD34+ cell ratio. The ratio was higher only when both platelets and reticulocytes increased in parallel with WBC. We conclude that sufficient PBSC collection is possible after conventional chemotherapy using G‐CSF, when hematopoietic recovery is parallel, without the use of high‐dose chemotherapy.
Efficacy of synchronized IMV on weaning neonates from the ventilator
Authors: MIZUNO KATSUMI , TAKEUCHI TOSHIO , ITABASHI KAZUO , OKUYAMA KAZUO 
Published: January 2011

AbstractThis study evaluated the efficacy of a new patient‐triggered ventilator that triggered the patient's inspiratory effort by detecting the change in airflow by means of a ‘hot wire’ anemometer. This ventilator was used in both the conventional and the synchronzied intermittent mandatory ventilation (SIMV) modes in seven neonates. Values for blood gas, spontaneous breathing rate, tidal volume of spontaneous breaths and minute volume were compared in all seven neonates. The resistive work of spontaneous breathing in five neonates, obtained with synchronized intermittent mandatory ventilation was compared with the values obtained using conventional mechanical ventilation on the previous day of weaning from the ventilator. At each the inflation time studied (0.4, 0.3, 0.24 s), all mechanical breath occurred synchronously with infants' inspiratory efforts. The median trigger delay was 80 ms. Oxygenation was improved on the new system compared with the conventional system. Tidal volume of spontaneous breathing and minute volume were increased with SIMV compared with conventional mechanical ventilation, although the resistive work of spontaneous breathing was decreased with SIMV. The tidal volume of spontaneous breaths was more constant with SIMV versus conventional mechanical ventilation. Thus, the airway flow‐triggered SIMV may lessen inspiratory muscle fatigue during weaning process.We conclude that the SIMV is useful in weaning neonates from the ventilator.oxygenationresistive work of spontaneous breathingsynchronized intermittent mandatory ventilationwearing process.
A new oral margarine loading test in children
Authors: SHIMIZU TOSHIAKI , YAMASHIRO YUICHIRO , YABUTA KEIJIRO 
Published: January 2011

AbstractMargarine (2g/kg) was orally administered to 39 children with normal fat digestion and absorption (control group) and 17 children with abnormal fat digestion and absorption (malabsorbed group). The serum triglyceride (TG) level was serially determined by capillary blood sampling. In the control group, the TG level was significantly elevated after 2, 3 and 4 h as compared with the level before margarine ingestion (basal TG). The maximum TG elevation rate [MTER ‐ (maximum TG‐basal TG)/basal TG x 100] was > 50% in all but three children. In contrast, a significant increase in TG was not observed in the malabsorbed group after margarine ingestion, and the MTER was < 50% in all but two children. The MTER was significantly lower in children with a high microscopic score of fecal fat than that in children with a low score. This oral margarine loading test is a simple and semi‐quantitative evaluation of fat digestion and absorption, and is considered to be useful for the assessment of the presence or severity of fat maldigestion and malabsorption in children.
Differential alpha‐smooth muscle actin expression in a gastric epithelioid leiomyosarcoma in a 14 year old girl
Authors: HOSOI HAJIME , SUGIMOTO TOHRU , FUSHIKI SHINJI , MINE HIROSHI , KADONO YOHSUKE , SAWADA TADASHI 
Published: January 2011

AbstractLeiomyosarcoma in childhood is very rare. This report describes the immunohistochemical study of a gastric epithelioid leiomyosarcoma in a 14 year old girl. Microscopically, the tumor showed two distinct histological patterns within the same tumor: small epithelioid cells forming a lobular pattern and spindle‐shaped cells forming interlacing fascicles, but no distinct border divided the two types of cells. Immunohistochemically, vimentin was positive in all tumor cells; a‐smooth muscle actin, however, was negative in small epithelioid cells and positive in spindle‐shaped cells. The data revealed that the tumor had components showing two stages of morphological and biochemical differentiation. This case indicates that leiomyosarcomas might lose smooth muscle actin during malignant transformation; or, alternatively, they might arise from a primitive type of smooth muscle cell and some of them could spontaneously differentiate into mature smooth muscle cells, expressing this actin.
Renal impairment in very low birthweight infants following antenatal indomethacin administration
Authors: NISHIKUBO TOSHIYA , TAKAHASHI YUKIHIRO , NAKAGAWA YAEKO , KAWAGUCHI CHIHARU , NAKAJIMA MITSURU , ICHIJO MOTOHIKO , YOSHIOKA AKIRA 
Published: January 2011

AbstractThree cases of neonatal renal insufficiency in very low birthweight (VLBW) infants following repeated antenatal administration of indomethacin to prevent premature labor are reported. Three pregnant women received indomethacin (total doses of 150–850 mg) for 3–14 days from admission until delivery. The gestational ages and birthweights of the infants ranged from 24 to 28 weeks and 612 to 1432 g, respectively. Oliguria, early onset of hyperkalemia and prolonged renal dysfunction occurred after birth. Renal failure did not improve in one infant.Despite the efficacy of indomethacin for tocolysis in premature labor, VLBW infants born after repeated maternal administration near the time of delivery may have developed impairment of the premature kidney.
A case report of placental hemangioma resulting in severe fetal distress
Authors: ITO MASAZUMI , KUMAMOTO TADASI , YAMAMOTO HATSUMI , NOMURA TOYOKI , MATSUMOTO TAKAFUMI , TAKI MITIO , TOYODA NAGAYASU 
Published: January 2011

AbstractA 30 year old female was admitted twice during her pregnancy for genital bleeding. An ultrasonography at 19 weeks gestation showed several nodules on the chorionic plate. Cardiotocography during labor at 37 weeks gestation indicated a non‐reactive pattern. A 2230 g infant girl was delivered soon after rupture of membranes with asphyxia and hydrops fetalis. The placenta weighed 600 g with a maximal thickness of 6 cm. Nodules occupied about one‐third of the placenta and were diagnosed as placental hemangiomas. Postnatal examination revealed that the infant had disseminated intravascular coagulation and cardiac failure. This baby recovered after treatment in the intensive care unit, and was discharged home on the 52nd day of life.
Severe aplastic anemia in a patient with primary immunodeficiency
Authors: OHGA SHOUICHI , NAGASHIMA TAKAKO , NISHIZAKI MARI , HIRABARU CHIKAKO , INOUE TOSHIROU , ISE KEIKO , HARA TOSHIRO , UEDA KOHJI 
Published: January 2011

AbstractThis paper reports the case of a 6 year old boy with primary immunodeficiency disease, whose marrow cells showed dyshematopoietic features, that was subsequently transformed to severe aplastic anemia. He was first diagnosed as having congenital immunodeficiency comprising deficiency of immunoglobulin A (IgA), IgG2 and IgG4, depressed mitogen responses, lymphopenia with inverted CD4/CD8 ratio and an increased proportion of T‐cell receptor γ/δ‐bearing cells. Cytogenetic study of the peripheral blood showed a normal karyotype of 46, XY, but that of the marked hypoplastic marrow demonstrated one cell with monosomy 7 and another with trisomy 8 in the 20 cells examined.
Haemophilus influenzae type D infection and JgG2 deficiency in a patient with chronic mucocutaneous candidiasis
Authors: SAKANO TAKASHI , HAMASAKI TAKASHI , MORI MIKIO , TAKAHASHI HIROKO 
Published: January 2011

AbstractA 14 year old boy with chronic mucocutaneous candidiasis and persistent pulmonary infection caused by Haemophylus influenzae and Streptococcus pneumoniae is reported. Initial bacterial culture studies showed H. influenzae type B and S. pneumoniae as causative agents. H. influenzae type D was constantly isolated from the patient's sputum. Abnormally low levels of serum immunoglobulin G2 (IgG2) found in the patient may have contributed to the pulmonary infection and H. influenzae type D may be an important causative agent in immunodeficient patients.
Cough variant asthma: A case study with antigen, histamine and methacholine inhalation challenge tests
Authors: MORIKAWA A. , MITSUHASHI M. , TABATA H. , SEKI Y. , MOCHIZUKI H. , TOKUYAMA K. , KUROUME T. 
Published: January 2011

AbstractThis paper presents a 7 year old girl with cough variant asthma, in which house dust challenge test provoked fine crackles on auscultation without any changes of lung function test. Pretreatment with dl‐chlorpheniramine maleate for 7 days inhibited the appearance of fine crackles. On histamine inhalation test, fine crackles and rhonchi appeared on auscultation without bronchoconstriction (% fall of one‐second forced expiratory volume < 10%). These data suggest that the cause of cough variant asthma is mainly an immunoglobulin E‐mediated allergic reaction in the mucus secreting system.
Rapid detection of phenylketonuria mutations by non‐radioactive single‐strand conformation polymorphism analysis
Authors: YAO YEQI , MATSUBARA YOICHI , NARISAWA KUNIAKI 
Published: January 2011

AbstractA non‐radioactive single‐strand conformation polymorphism (SSCP) method was used to detect various phenylketonuria (PKU) mutations in Japanese and Chinese patients. Arginine413‐to‐proline (R413P) mutation in exon 12 of the phenylalanine hydroxylase gene was identified in a Japanese patient by this method. The segregation of the R413P mutation in the proband's family was clearly demonstrated and the carrier status of each family member was determined. Analysis of DNA fragments containing exon 7 originated from Chinese patients revealed two mutations, arginine243‐to‐glutamine (R243Q) and arginine261‐to‐glutamine (R261Q), and a polymorphism, valine245‐to‐valine (V245V). Although R261Q has been identified previously among Caucasian subjects, this report is the first to describe this mutation among Orientals. Since the non‐radioactive SSCP method employs pre‐cast acrylamide gels and pre‐made gel buffer strips combined with semi‐automated temperature‐controlled electrophoresis, it can be performed without much expertise in molecular biological techniques. The ability of this method to detect various mutations as demonstrated in this study and its ease of use make it feasible to detect PKU mutations in a routine DNA diagnostic laboratory.
Prevalence of Chlamydia pneumoniae in acute lower respiratory infection in the pediatric population in Japan
Authors: OUCHI KAZUNOBU , NAKAZAWA TERUKO , KARITA MIKIO , KANEHARA YOJI 
Published: January 2011

AbstractChlamydia pneumoniae has been established as an important etiologic agent of acute respiratory tract infection in humans, especially in adults. However, there is little information available on C. pneumoniae infection in the pediatric age group. The microimmunofluorescence test and Chlamydia pneumoniae‐specific polymerase chain reaction (PCR) method were applied to reveal the role of C. pneumoniae as an etiologic agent of acute lower respiratory tract infection in children in Japan. Among 136 patients aged between 2 months and 15 years with acute lower respiratory tract infection, five patients with pneumonia were found to have recent C. pneumoniae infection by the microimmunofluorescence test. C. pneumoniae‐specific PCR products were detected from specimens of four patients among these five. Neither C. trachomatis nor C. psittaci infection was found in this population. These results suggest that C. pneumoniae is an important causative agent of acute childhood pneumonia and may be the most prevalent pathogen among the genus Chlamydia that causes acute lower respiratory disease in this age group in Japan.
Investigation of melena and hematochezia as the chief complaint of gastrointestinal bleeding in pediatric surgical patients
Authors: UNO TAKEJI , HARADA YUKIO , KIMURA TAIZO , MOCHIDA YOSHIAKI , GOTO KEIGO , MIYATANI KAORU , KUBO SEIICHI , LI XIAO RANG 
Published: January 2011

AbstractThe causes of melena or hematochezia in 48 pediatric patients were examined. Malrotation with volvulus was an important cause of hemorrhage during the newborn period, and intussusception was very typical in patients aged from 1 month to 1 year. Polyps of the rectum and colon were the most common causes of melena or hematochezia in patients older than 1 year. No cause of melena or hematochezia could be identified in 11 children. Ten patients have remained in good health with no further episodes of melena or hematochezia. Localized multiple polyps of the rectum with focal carcinoma were detected in only one patient. In general, although no further investigation is required after detection of the cause of bleeding and its successful treatment, it should be kept in mind that gastrointestinal malignancy can occur in children.
Two cases of insulin‐dependent diabetes mellitus under insulin treatment with slow height velocity: Relationship of growth hormone‐binding protein, metabolic control and growth
Authors: MURATA ATSUSHI , YASUDA TOSHIYUKI , NIIMI HIROO 
Published: January 2011

AbstractInadequate blood sugar control in children with insulin‐dependent diabetes mellitus (IDDM) sometimes results in low insulin‐like growth factor‐I (IGF‐I) and sluggish height growth. High affinity growth hormone‐binding protein (GHBP), which is identical to the extracellular domain of growth hormone (GH) receptor, is present in the human sera. We have determined GHBP activity in two cases of poorly controlled IDDM with low height velocity in relation to metabolic control in order to determine the mechanism of resistance to GH in this condition, as indicated by low levels of GH‐dependent growth factor IGF‐I in the face of high serum GH levels. GHBP activity was within the normal range in two cases of IDDM with slow height velocity, low IGF‐I and high hemoglobin‐A1. In both cases, improved blood sugar control normalized IGF‐I to result in accelerated height velocity without a major change in GHBP levels. These results may indicate either normal peripheral GH receptor or normal free portion of serum GH, and may suggest that the major defect in slow growth in poorly controlled diabetes is due to the post GH receptor.
A case of bacterial meningitis complicated by posttraumatic cerebrospinal fluid rhinorrhea
Authors: WADA NORIKO , TAKEUCHI YOSHIHIRO , FUJII MASAYO , FUJIWARA FUMIHIRO , ODANI ISAO , SAWADA TADASHI 
Published: January 2011

AbstractThis paper reports an 11 year old boy with bacterial meningitis accompanied by post‐traumatic cerebrospinal fluid (CSF) rhinorrhea. Streptococcus pneumoniae was cultured from CSF. The clinical course was very rapid before admission and his age relatively high for usual bacterial meningitis. Consequently, we examined the cause in detail. Immunological findings were within normal limits. Although routine graphic examinations, such as cranial X‐ray photography, horizontal cranial computed tomography (CT) and magnetic resonance imaging, could not demonstrate a bone defect, both coronal thin‐section cranial CT scanning and radioactive isotope counting by means of cotton packed into the nasal cavity were useful for detecting CSF rhinorrhea. In a case of atypical meningitis, the past history should be examined with caution and coronal thin‐section CT should be performed.
A case of cytomegalovirus mononucleosis associated with pleural effusion
Authors: KATO YOKO , MIYATA ICHIRO , SAKUMA SHUYA , KATAYAMA AKIRA , TOYODA SHIGERU , KOBAYASHI SHIN'ICHI , OKABE NOBUHIKO , MAEKAWA KIHEI 
Published: January 2011

AbstractA 5 year old boy had a spiky fever accompanied by a mild pharyngitis, cervical lymphadenopathy and hepatosplenomegaly. Laboratory findings revealed leukocytosis with 26% atypical lymphocytes, and liver dysfunction. A chest X‐ray showed pneumonia and a considerable amount of pleural effusion. Serum antibody titers for cytomegalovirus (CMV) were elevated significantly and CMV‐DNA (polymerase chain reaction) was detected in the pleural effusion. Only 13 cases of pleural effusion associated with infectious mononucleosis have been reported previously in the literature, but there was no documentation that proved CMV infection. The case reported here suggests that the pleural effusion was caused by the infiltration of mononuclear cells to the pleura as a result of systemic inflammation, and the possible alternative of host immune response against CMV was related to recent Varicella zoster virus infection.
Balloon pulmonary valvuloplasty for double outlet right ventricle with valvular pulmonary stenosis
Authors: AKIBA TOMOHARU , SATO TETSUO , YOSHIKAWA MASARU , NAKASATO MITSURU , SUZUKI HIROSHI , SATO SATOSHI 
Published: January 2011

AbstractA male neonate presented with cyanosis. Echocardiograms showed a double outlet right ventricle, an absent outlet septum, a subaortic ventricular septal defect and valvular pulmonary stenosis. Balloon pulmonary valvuloplasty was performed at 19 days of age, resulting in an immediate increase of the systemic arterial oxygen saturation from 67 to 87%. As far as could be found, this is the first report of a patient with a double outlet right ventricle without the outlet septum who was clinically observed and underwent balloon pulmonary valvuloplasty.
A case of siblings with Meckel's diverticulum diagnosed before operation
Authors: MATSUKUMA YOSHINORI , MATSUO YUSAKU , SAKAGUCHI MINAKO , YAMADA TAKASHI , HIRATA TOMOSHIGE , TANAKA NOBUO , MORITA JUN , ARAKI HISA‐AKI , SUE KOHJI , KANEKO TAKASHI , NAKANISHI KAZUO 
Published: January 2011

AbstractThe authors encountered a sibling case of Meckel's diverticulum in 9 and 11 year old Japanese boys. They were diagnosed using contrast examination and 99mTc‐pertechnetate scintigraphy before operation. The familial tendency is one in every 2500 families. Only 15 cases of familial occurence have been reported by five authors.
Hepatic calcification in an anicteric patient with biliary atresia after hepatic portoenterostomy
Authors: MATSUI AKIRA , NAKAMURA MICHIRU , AIHARA TOSHINORI , FURUSE MAKOTO , SASAKI NOBUHIKO , ARAKAWA YOICHI , YANAGISAWA MASAYOSHI , MAKINO SHUN‐ICHI 
Published: January 2011

AbstractHepatic calcification was found in an anicteric infant with biliary atresia after hepatic portoenterostomy. When she was 2 years of age, ultrasonography detected an echogenic structure with acoustic shadowing. Computerized tomography located it at the proximal portion of Segment 6 in the right lobe. Although both techniques failed to exhibit dilatation of the peripheral bile ducts, we believe intrahepatic cholelithiasis provides the most likely diagnosis. Although seven cases with biliary atresia were reported to have this complication, all of them were icteric when the diagnosis was confirmed. The patient should be carefully followed up since the succeeding stones may cause jaundice by obstructing the main duct.