bluuebunny/crossref_metadata_2025_split huggingface dataset:
Achieving an Optimal Outcome After Stage 1 Palliation for Hypoplastic Left Heart Syndrome and Variants: Frequency, Associated Factors, and Subsequent Outcomes
Authors: Shalhoub Khayri , Heydarian Haleh C. , Hanke Samuel P. , Cnota James F. , Stein Laurel H. , Tepe Brooke , Hill Garick D. 
Published: June 2024

Background
We sought to measure frequency of achieving an optimal outcome after stage 1 palliation (S1P) for hypoplastic left heart syndrome and variants, determine factors associated with optimal outcomes, and compare outcomes after stage 2 palliation (S2P) using the National Pediatric Cardiology Quality Improvement Collaborative database (2008–2016).

Methods and Results

This is a retrospective cohort study with optimal outcome defined a priori as meeting all of the following: (1) discharge after S1P in <19 days (top quartile), (2) no red flag or major event readmissions before S2P, and (3) performing S2P between 90 and 240 days of age. Optimal outcome was achieved in 256 of 2182 patients (11.7%). Frequency varied among centers from 0% to 25%. Factors independently associated with an optimal outcome after S1P were higher gestational age (odds ratio [OR], 1.1 per week [95% CI, 1.0–1.2];
P
=0.02); absence of a genetic syndrome (OR, 2.5 [95% CI, 1.2–5];
P
=0.02); not requiring a post‐S1P catheterization (OR, 2.7 [95% CI, 1.5–4.8];
P
=0.01), intervention (OR, 1.5 [95% CI, 1.1–2];
P
=0.006), or a procedure (OR, 4.5 [95% CI, 2.8–7.1];
P
<0.001) before discharge; and not having a post‐S1P complication (OR, 2.7 [95% CI, 1.9–3.7];
P
<0.001). Those with an optimal outcome after S1P had improved S2P outcomes including shorter length of stay, less ventilator days, shorter bypass time, and fewer postoperative complications.

Conclusions
Identifying patients at lowest risk for poor outcomes during the home interstage period could shift necessary resources to those at higher risk, alter S2P postoperative expectations, and improve quality of life for families at lower risk.
Youth and Prayer in a Secular Age
Authors: Ellis Wesley W. 
Published: June 2024

Abstract
This article diagnoses the difficulty of prayer for young people inhabiting a secular society and offers the beginnings of a theology of prayer for this context. For young people in the United States, our secular age is defined by immanence, excessive positivity, and dynamic stabilization that constitute anxiety as a primary motivator that leads to burnout and exhaustion. Prayer as communion with God offers a corrective that can relieve anxiety and offer renewal. For this, prayer must not be thought primarily as a human action, but a divine action. In prayer, we do not grasp God, we are grasped by God. As such, prayer is a grace young people need, and its teaching must become the primary pastoral vocation in a secular age.
Investigative studies on natural fiber reinforced composites for automotive bumper beam applications
Authors: Wagh Jayashri Pawar , Malagi Ravindra R , Madgule Mahadev 
Published: June 2024

The automotive sector wants lighter, recyclable, degradable, and sustainable composite materials. In the last few decades, natural fiber composite has been used in a wide range. The natural plant-based fibers are reinforced into polymeric composite materials, giving significant mechanical properties compared to conventional material. This work selects flax and sisal fibers due to higher strength and Young’s modulus. Mechanical behavior such as tensile, flexural, and impact strength are evaluated. Mechanical behavior results are considered in the simulation to determine the performance of an automotive bumper beam. The tensile strength of the flax composite is 90% higher than that of the sisal composite. Flexural strength is 17% higher in a flax composite, whereas energy absorption is nearly identical in a flax and sisal composite. The flax composite has a maximum 204.9 MPa tensile strength, 157.98 MPa flexural strength, and 45 J energy absorption. The mechanical behavior results are validated with simulation results in the ANSYS workbench for static analysis. The results indicate that the flax fiber reinforced composite with 43:57 of a flax-matrix ratio performs better as it can be used as a structural material. A morphological study shows the uniform distribution of matrix material and fiber orientation.
Reconstructive Septorhinoplasty for Surgical Correction of Tessier's No. 0 Cleft in Adult Patients
Authors: Sazgar Mehr A. , Sazgar Amir A. 
Published: June 2024

Background Tessier No. 0 cleft, characterized by a bifid nose, significantly impacts facial development, imposing significant psychological and financial burdens on patients. Correction lacks consensus due to varied presentations and limited documentation of surgical treatments, notably in adults. Solution Loco-regional soft tissue flaps can address minor deformities, but establishing a robust skeletal framework is often crucial for permanent correction. Innovation This article introduces a novel and effective approach through reconstructive septorhinoplasty for addressing this anomaly.
Healthcare professionals’ perception on emergence of security threat using digital health technologies in healthcare delivery
Authors: Kissi Jonathan , Azakpah Godwin , Mensah Nathan Kumasenu , Dzamvivie Kennedy , Bosompem Twisty Ampofowaa , Wireko Victor Adu , Amoah-Anomah Grace 
Published: June 2024

Background The advancement of digital health technologies (DHTs) in the healthcare industry has revolutionized healthcare by improving efficiency and accessibility. The shift from paper-based records to electronic health records (EHRs) as a result of modern technologies introduced various security threats, endangering patient data privacy and system reliability. This study explores intricate security threats among healthcare professionals affecting DHT utilization. Methodology A structured questionnaire was designed to solicit for data from healthcare professionals on the existence of possible security threats, magnitude of physical and technical threats, and the extent to which the activities of staff and vendors affect the current DHTs being utilized in Cape Coast Teaching Hospital and Directorate of University Health Services at the University of Cape Coast. Purposive and convenience sampling techniques were employed to select healthcare professionals from various medical fields, and their views were captured for the study. Results Findings and data analyzed from the study revealed that technical threats, such as hacking, phishing, malware, and encryption weaknesses, pose more substantial dangers to DHTs compared to physical threats. The study explored viable strategies to prevent unauthorized access to DHTs and safeguard patient information. Encouraging measures, such as encryption, multi-factor authentication, regular security training, and periodic password changes, emerged as promising methods in addressing DHT security threats. Conclusion The study emphasizes the necessity of robust security measures and regular updates to effectively counter the emerging security threats. It underscores the critical necessity for a comprehensive protocol to enhance DHT security, addressing physical, technical, and personnel-related threats.
PhyloNext: a pipeline for phylogenetic diversity analysis of GBIF-mediated data
Authors: Mikryukov Vladimir , Abarenkov Kessy , Laffan Shawn , Robertson Tim , McTavish Emily Jane , Jeppesen Thomas Stjernegaard , Waller John , Blissett Matthew , Kõljalg Urmas , Miller Joseph T. 
Published: June 2024

Abstract
Background
Understanding biodiversity patterns is a central topic in biogeography and ecology, and it is essential for conservation planning and policy development. Diversity estimates that consider the evolutionary relationships among species, such as phylogenetic diversity and phylogenetic endemicity indices, provide valuable insights into the functional diversity and evolutionary uniqueness of biological communities. These estimates are crucial for informed decision-making and effective global biodiversity management. However, the current methodologies used to generate these metrics encounter challenges in terms of efficiency, accuracy, and data integration.

Results
We introduce PhyloNext, a flexible and data-intensive computational pipeline designed for phylogenetic diversity and endemicity analysis. The pipeline integrates GBIF occurrence data and OpenTree phylogenies with the Biodiverse software. PhyloNext is free, open-source, and provided as Docker and Singularity containers for effortless setup. To enhance user accessibility, a user-friendly, web-based graphical user interface has been developed, facilitating easy and efficient navigation for exploring and executing the pipeline. PhyloNext streamlines the process of conducting phylogenetic diversity analyses, improving efficiency, accuracy, and reproducibility. The automated workflow allows for periodic reanalysis using updated input data, ensuring that conservation strategies remain relevant and informed by the latest available data.

Conclusions
PhyloNext provides researchers, conservationists, and policymakers with a powerful tool to facilitate a broader understanding of biodiversity patterns, supporting more effective conservation planning and policy development. This new pipeline simplifies the creation of reproducible and easily updatable phylogenetic diversity analyses. Additionally, it promotes increased interoperability and integration with other biodiversity databases and analytical tools.
Transcriptome analysis of the Japanese eel (Anguilla japonica) during larval metamorphosis
Authors: Sudo Ryusuke , Asakura Taiga , Ishikawa Takashi , Hatakeyama Rui , Fujiwara Atushi , Inoue Komaki , Mochida Keiichi , Nomura Kazuharu 
Published: June 2024

Abstract
Background
Anguillid eels spend their larval period as leptocephalus larvae that have a unique and specialized body form with leaf-like and transparent features, and they undergo drastic metamorphosis to juvenile glass eels. Less is known about the transition of leptocephali to the glass eel stage, because it is difficult to catch the metamorphosing larvae in the open ocean. However, recent advances in rearing techniques for the Japanese eel have made it possible to study the larval metamorphosis of anguillid eels. In the present study, we investigated the dynamics of gene expression during the metamorphosis of Japanese eel leptocephali using RNA sequencing.

Results
During metamorphosis, Japanese eels were classified into 7 developmental stages according to their morphological characteristics, and RNA sequencing was used to collect gene expression data from each stage. A total of 354.8 million clean reads were generated from the body and 365.5 million from the head, after the processing of raw reads. For filtering of genes that characterize developmental stages, a classification model created by a Random Forest algorithm was built. Using the importance of explanatory variables feature obtained from the created model, we identified 46 genes selected in the body and 169 genes selected in the head that were defined as the “most characteristic genes” during eel metamorphosis. Next, network analysis and subsequently gene clustering were conducted using the most characteristic genes and their correlated genes, and then 6 clusters in the body and 5 clusters in the head were constructed. Then, the characteristics of the clusters were revealed by Gene Ontology (GO) enrichment analysis. The expression patterns and GO terms of each stage were consistent with previous observations and experiments during the larval metamorphosis of the Japanese eel.

Conclusion
Genome and transcriptome resources have been generated for metamorphosing Japanese eels. Genes that characterized metamorphosis of the Japanese eel were identified through statistical modeling by a Random Forest algorithm. The functions of these genes were consistent with previous observations and experiments during the metamorphosis of anguillid eels.
The nuclear and mitochondrial genome assemblies of Tetragonisca angustula (Apidae: Meliponini), a tiny yet remarkable pollinator in the Neotropics
Authors: Ferrari Rafael Rodrigues , Ricardo Paulo Cseri , Dias Felipe Cordeiro , de Souza Araujo Natalia , Soares Dalliane Oliveira , Zhou Qing-Song , Zhu Chao-Dong , Coutinho Luiz Lehmann , Arias Maria Cristina , Batista Thiago Mafra 
Published: June 2024

Abstract
Background
The field of bee genomics has considerably advanced in recent years, however, the most diverse group of honey producers on the planet, the stingless bees, are still largely neglected. In fact, only eleven of the ~ 600 described stingless bee species have been sequenced, and only three using a long-read (LR) sequencing technology. Here, we sequenced the nuclear and mitochondrial genomes of the most common, widespread and broadly reared stingless bee in Brazil and other neotropical countries—Tetragonisca angustula (popularly known in Brazil as jataí).

Results
A total of 48.01 Gb of DNA data were generated, including 2.31 Gb of Pacific Bioscience HiFi reads and 45.70 Gb of Illumina short reads (SRs). Our preferred assembly comprised 683 contigs encompassing 284.49 Mb, 62.84 Mb of which (22.09%) corresponded to 445,793 repetitive elements. N50, L50 and complete BUSCOs reached 1.02 Mb, 91 contigs and 97.1%, respectively. We predicted that the genome of T. angustula comprises 17,459 protein-coding genes and 4,108 non-coding RNAs. The mitogenome consisted of 17,410 bp, and all 37 genes were found to be on the positive strand, an unusual feature among bees. A phylogenomic analysis of 26 hymenopteran species revealed that six odorant receptor orthogroups of T. angustula were found to be experiencing rapid evolution, four of them undergoing significant contractions.

Conclusions
Here, we provided the first nuclear and mitochondrial genome assemblies for the ecologically and economically important T. angustula, the fourth stingless bee species to be sequenced with LR technology thus far. We demonstrated that even relatively small amounts of LR data in combination with sufficient SR data can yield high-quality genome assemblies for bees.
Comprehensive identification and systematical characterization of BRX gene family and the functional of GhBRXL5A in response to salt stress
Authors: Zhu Shouhong , Li Yan , Chen Wei , Yao Jinbo , Fang Shengtao , Pan Jingwen , Wan Wenting , Tabusam Javaria , Lv Youjun , Zhang Yongshan 
Published: June 2024

Abstract
Background
BRVIS RADIX (BRX) family is a small gene family with the highly conserved plant-specific BRX domains, which plays important roles in plant development and response to abiotic stress. Although BRX protein has been studied in other plants, the biological function of cotton BRX-like (BRXL) gene family is still elusive.

Result
In this study, a total of 36 BRXL genes were identified in four cotton species. Whole genome or segmental duplications played the main role in the expansion of GhBRXL gene family during evolutionary process in cotton. These BRXL genes were clustered into 2 groups, α and β, in which structural and functional conservation within same groups but divergence among different groups were found. Promoter analysis indicated that cis-elements were associated with the phytohormone regulatory networks and the response to abiotic stress. Transcriptomic analysis indicated that GhBRXL2A/2D and GhBRXL5A/5D were up/down-regulated in response to the different stress. Silencing of GhBRXL5A gene via virus-induced gene silencing (VIGS) improved salt tolerance in cotton plants. Furthermore, yeast two hybrid analysis suggested homotypic and heterotypic interactions between GhBRXL1A and GhBRXL5D.

Conclusions
Overall, these results provide useful and valuable information for understanding the evolution of cotton GhBRXL genes and their functions in salt stress.
The challenges associated with the prevention of smuggling and counterfeiting health goods in Iran
Authors: Mahmoudi Meymand Farzaneh , Takian Amirhossein , Jaafaripooyan Ebrahim 
Published: June 2024

Abstract
Background
Smuggling health goods given the importance and critical nature of health services should be undeniably addressed and controlled by all countries. This issue is especially more widespread in developing countries with more damaging consequences. This paper therefore aims to identify and analyze the challenges of preventing smuggling of health goods in Iran.

Method
Within this qualitative study, we conducted face-to-face, semi-structured interviews with 30 purposefully recruited key informants and stakeholders in the detection, prevention, and combating of health goods smuggling. Each interview was analyzed thematically, using an inductive approach to generate codes, then categorized and presented in the form of main themes and sub-themes. Maxqda 11 assisted in coding, analysis, and data management.

Results
Three main themes emerged representing the challenges of prevention of smuggling in Iran in the areas of anti-smuggling policy development, including categories of inefficient policy and plan, and failure to reach agenda; policy implementation; categorized into actors, resources and instruments, and implementation guarantee; and finally monitoring and evaluation; including, procedures and practices, and the role of surveyors.

Conclusion
Prevention of smuggling health goods proves to be a highly complex, challenging, and multi-faceted practice. Therefore, strengthening policy-making, regulatory frameworks, and facilitation functions about smuggling, counterfeiting, and corruption should be promoted in parallel.
Effectiveness of cognitive rehearsal programs for the prevention of workplace bullying among hospital nurses: a systematic review and meta-analysis
Authors: Jeong Yulliana , Jung Hye Sun , Baek Eun Mi 
Published: June 2024

Abstract
Background
To solve the problem of workplace bullying among nurses, it is necessary to review the effects of interventions and generalize the findings. We conducted a systematic literature review and meta-analysis to evaluate the effects of cognitive rehearsal programs on workplace bullying among hospital nurses.

Methods
Data were collected from March 30 to April 11, 2021, and 11,048 journal articles published in South Korea and internationally were examined across eight databases. Nine articles were selected for inclusion in the systematic literature review; five of the nine studies were included in the meta-analysis. For randomized controlled trials, the risk of bias was evaluated, and for non-randomized controlled trials, the study quality was evaluated using the Risk of Bias for Non-randomized Studies version 2.0. Egger’s regression test was performed to determine publication bias.

Results
Of the nine articles selected for this study, two were randomized controlled trials and seven were non-randomized controlled trials. The I2 value was 18.9%, indicating non-significant heterogeneity. The overall effect size of the cognitive rehearsal programs was -0.40 (95% confidence interval: -0.604 to -0.196; Z = -3.85; p = .0001) in a random-effects model, indicating a large effect size with statistical significance.

Conclusions
Therefore, cognitive rehearsal programs that address workplace bullying among hospital nurses are effective. Health policymakers must implement cognitive rehearsal programs in a policy manner to address the problems of bullying in the workplace.
Risk of breakthrough infection and hospitalisation after COVID-19 primary vaccination by HIV status in four Italian regions during 2021
Authors: Mateo-Urdiales Alberto , Fabiani Massimo , Mayer Flavia , Sacco Chiara , Belleudi Valeria , Da Cas Roberto , Fotakis Emmanouil Alexandros , De Angelis Luigi , Cutillo Maria , Petrone Daniele , Morciano Cristina , Cannone Andrea , Del Manso Martina , Riccardo Flavia , Bella Antonino , Menniti-Ippolito Franscesca , Pezzotti Patrizio , Spila Alegiani Stefania , Massari Marco 
Published: June 2024

Abstract
Background
As of 2024, vaccination remains the main mitigation measure against COVID-19, but there are contradictory results on whether people living with HIV (PLWH) are less protected by vaccines than people living without HIV (PLWoH). In this study we compared the risk of SARS-CoV-2 infection and COVID-19 hospitalisation following full vaccination in PLWH and PLWoH.

Methods
We linked data from the vaccination registry, the COVID-19 surveillance system and from healthcare/pharmacological registries in four Italian regions. We identified PLWH fully vaccinated (14 days post completion of the primary cycle) and matched them at a ratio of 1:4 with PLWoH by week of vaccine administration, age, sex, region of residence and comorbidities. Follow-up started on January 24, 2021, and lasted for a maximum of 234 days. We used the Kaplan-Meier estimator to calculate the cumulative incidence of infection and COVID-19 hospitalisation in both groups, and we compared risks using risk differences and ratios taking PLWoH as the reference group.

Results
We matched 42,771 PLWH with 171,084 PLWoH. The overall risk of breakthrough infection was similar in both groups with a rate ratio (RR) of 1.10 (95% confidence interval (CI):0.80–1.53). The absolute difference between groups at the end of the study period was 8.28 events per 10,000 person-days in the PLWH group (95%CI:-18.43-40.29). There was a non-significant increase the risk of COVID-19 hospitalisation among PLWH (RR:1.90; 95%CI:0.93–3.32) which corresponds to 6.73 hospitalisations per 10,000 individuals (95%CI: -0.57 to 14.87 per 10,000).

Conclusions
Our findings suggest PLWH were not at increased risk of breakthrough SARS-CoV-2 infection or COVID-19 hospitalisation following a primary cycle of mRNA vaccination.