bluuebunny/crossref_metadata_2025_split huggingface dataset:
The design of long‐term effective uranium bioremediation strategy using a community metabolic model
Authors: Zhuang K. , Ma E. , Lovley Derek R. , Mahadevan Radhakrishnan 
Published: April 2012

AbstractAcetate amendment at uranium contaminated sites in Rifle, CO. leads to an initial bloom of Geobacter accompanied by the removal of U(VI) from the groundwater, followed by an increase of sulfate‐reducing bacteria (SRBs) which are poor reducers of U(VI). One of the challenges associated with bioremediation is the decay in Geobacter abundance, which has been attributed to the depletion of bio‐accessible Fe(III), motivating the investigation of simultaneous amendments of acetate and Fe(III) as an alternative bioremediation strategy. In order to understand the community metabolism of Geobacter and SRBs during artificial substrate amendment, we have created a genome‐scale dynamic community model of Geobacter and SRBs using the previously described Dynamic Multi‐species Metabolic Modeling framework. Optimization techniques are used to determine the optimal acetate and Fe(III) addition profile. Field‐scale simulation of acetate addition accurately predicted the in situ data. The simulations suggest that batch amendment of Fe(III) along with continuous acetate addition is insufficient to promote long‐term bioremediation, while continuous amendment of Fe(III) along with continuous acetate addition is sufficient to promote long‐term bioremediation. By computationally minimizing the acetate and Fe(III) addition rates as well as the difference between the predicted and target uranium concentration, we showed that it is possible to maintain the uranium concentration below the environmental safety standard while minimizing the cost of chemical additions. These simulations show that simultaneous addition of acetate and Fe(III) has the potential to be an effective uranium bioremediation strategy. They also show that computational modeling of microbial community is an important tool to design effective strategies for practical applications in environmental biotechnology. Biotechnol. Bioeng. 2012; 109: 2475–2483. © 2012 Wiley Periodicals, Inc.
Fluorescent silica particles for monitoring oxygen levels in three‐dimensional heterogeneous cellular structures
Authors: Acosta Miguel A. , Velasquez Melissa , Williams Katelyn , Ross Julia M. , Leach Jennie B. 
Published: April 2012

AbstractBacterial biofilms are a major obstacle challenging the development of more effective therapies to treat implant infections. Oxygen availability to bacterial cells has been implicated in biofilm formation and planktonic cell detachment; however, there are insufficient tools available to measure oxygen concentrations within complex three‐dimensional structures with ∼1 µm resolution. Such measurements may complement measures of biofilm structure and cell activity to provide a more comprehensive understanding of biofilm biology. Thus, we developed oxygen‐sensing microparticles specifically designed to characterize oxygen transport through the volume of bacterial biofilms. The Stöber method was used to synthesize monodisperse silica microparticles of approximately the same size as a bacterium (∼1 µm). Two fluorophores, oxygen‐sensitive Ru(Ph2phen3)Cl2, and the reference fluorophore Nile blue chloride were immobilized on the surface of the particles. We demonstrate application of the microparticles toward measuring the oxygen concentration profiles within a live Staphylococcus aureus biofilm. Biotechnol. Bioeng. 2012; 109: 2663–2670. © 2012 Wiley Periodicals, Inc.
Electrochemotherapy for disseminated superficial metastases from malignant melanoma
Authors: Campana L G , Valpione S , Mocellin S , Sundararajan R , Granziera E , Sartore L , Chiarion-Sileni V , Rossi C R 
Published: April 2012

Abstract

Background
The aim of the study was to determine predictive factors for effectiveness, toxicity and local disease control in patients with malignant melanoma treated with bleomycin-based electrochemotherapy (ECT).

Methods
Electrochemotherapy was offered to patients with superficially disseminated melanoma metastases unsuitable for resection and unresponsive to chemotherapy.

Results
Eighty-five patients were treated with up to six ECT cycles with minimal, mainly dermatological, toxicity. One month after the first ECT, an objective response was observed in 80 patients (94 per cent). After retreatment because of a partial response in 39 patients, a complete response was achieved in 19 patients. Among the 41 (48 per cent) complete responders at first ECT, 19 patients received a second cycle because of new lesions after a median of 6 (range 2–14) months. After a median follow-up of 26 months, six patients experienced local recurrence with a 2-year local progression-free survival rate of 87 per cent. In multivariable analysis, significant predictive factors for response were tumour size (odds ratio (OR) 0·23, 95 per cent confidence interval (c.i.) 0·19 to 0·86; P = 0·003) and number of lesions (OR 0·38, 0·28 to 0·88; P = 0·002). An increasing number of electrode applications (hazard ratio (HR) 2·18, 95 per cent c.i. 1·22 to 3·44; P = 0·041) and ECT cycles (HR 0·46, 0·22 to 0·95; P = 0·005) were predictors of local control. There were no predictors of toxicity. Melanoma thickness and lower limb location of metastases were prognostic for survival.

Conclusion
The most suitable candidates for ECT were patients with few and small metastases on the lower limb treated with multiple electrode applications and ECT cycles.
Scenting movie theatre commercials: The impact of scent and pictures on brand evaluations and ad recall
Authors: Lwin May O. , Morrin Maureen 
Published: April 2012

ABSTRACTWe utilize a novel advertising context—commercials seen in a simulated movie theater setting while ambient scent is emitted into the atmosphere—to explore the effects of multisensory cues on brand evaluation and advertising recall. Although both pictorial and olfactory cues enhance brand evaluations and ad recall overall, we find that olfactory (vs pictorial) cues generate more positive feelings toward the brand and enhance recall to a greater extent. We also find that, after a long time delay, re‐experiencing the scent activates pictures' ability to facilitate recall. Theoretical and managerial implications are discussed.Copyright © 2012 John Wiley & Sons, Ltd.
Most accurate definition of a high femoral artery puncture: Aiming to better predict retroperitoneal hematoma in percutaneous coronary intervention
Authors: Tremmel Jennifer A. , Tibayan Yen D. , O'Loughlin Aiden J.C. , Chan Trevor , Fearon William F. , Yeung Alan C. , Lee David P. 
Published: April 2012

AbstractBackground: Retroperitoneal hematoma (RPH) increases morbidity and mortality in percutaneous coronary intervention (PCI). High femoral arteriotomy is an independent predictor of RPH, but the optimal angiographic criterion for defining a high puncture is unknown. Methods: We retrospectively identified 557 consecutive PCI cases with femoral angiograms. Arteriotomy sites were categorized as high based on three angiographic criteria: at or above the proximal third of the femoral head (criterion A), at or above the most inferior border of the inferior epigastric artery (criterion B), and at or above the origin of the inferior epigastric artery (criterion C). Cases of RPH were then identified. Results: Of the 557 PCI patients, 26 had a high femoral arteriotomy by criterion A, 17 by criterion B, and 6 by criterion C. Among these patients with a high arteriotomy, RPH occurred in four with criterion A, in three with criterion B, and in one with criterion C. Of the three criteria, criterion A most strongly correlated with RPH (odds ratio [OR] 96, 95% confidence interval [CI] 10.3–898.4; p < 0.0001) compared with criterion B (OR 58, 95% CI 8.9 to 372.6; p < 0.0001) or C (OR 27, 95% CI 2.6 to 290.1; p = 0.053). All criteria had high specificity (A, 96%; B, 97%; C, 99%), but the sensitivity was higher with criterion A (80%) than criterion B (60%) or C (20%), and statistically, the use of criterion A led to the most accurate risk‐stratification for RPH (A, κ = 0.79; B, κ = 0.59; C, κ = 0.19). Conclusions: Among the three common definitions of high arteriotomy, femoral artery puncture at or above the proximal third of the femoral head is the landmark that most accurately risk stratifies PCI patients for development of RPH. © 2012 Wiley Periodicals, Inc.
Treatment of type‐a aortic dissection using endoprosthesis occurring after coronary artery bypass surgery
Authors: Pires de Morais Gustavo , Rodrigues Alberto , Gama Vasco 
Published: April 2012

AbstractType‐A aortic dissection is a rare and often fatal complication following coronary artery bypass surgery (CABG). Corrective surgery seldom improves patient outcome. This report reviews and discusses endoprosthetic correction of type‐A aortic dissection. A case of a transluminal correction of acute type‐A aortic dissection one year after CABG in a 66‐year‐old male with a history of ischemic and severely compromised left ventricular function is presented. A prosthesis originally designed for the abdominal aorta was successfully used. Regular follow‐up was performed and nearly 3 years post prosthesis implantation the patient is stable in New York Heart Association class II. To the authors' knowledge there are no other literature reports of endoprosthetic correction of a type‐A aortic dissection in the context of CABG with saphenous grafts. © 2012 Wiley Periodicals, Inc.
Relationship between the intensity of heparin anticoagulation and clinical outcomes in patients receiving glycoprotein IIb/IIIa inhibitors during primary percutaneous coronary intervention in acute myocardial infarction
Authors: Rozenman Yoseph , Mehran Roxana , Witzenbichler Bernhard , Dangas George , Desaga Martin , Kochman Janusz , Nilsen Dennis W. , Finkelstein Ariel , Mosseri Morris , Stone Gregg W. 
Published: April 2012

AbstractObjectivesWe sought to determine the impact of the activated clotting time (ACT) in patients with ST elevation myocardial infarction (STEMI) undergoing primary percutaneous coronary intervention (PCI) with unfractionated heparin (UFH) and a glycoprotein IIb/IIIa inhibitor (GPI).BackgroundUFH+GPI is commonly used during primary PCI for STEMI. UFH anticoagulation is titrated with ACT.MethodsPatients randomized to UFH+GPI in HORIZONS‐AMI who underwent primary PCI are included (N = 1,624). Initial UFH bolus was 60 IU kg−1 (target ACT: 200–250 sec). Patients were divided into three tertiles of peak ACT (cutoffs 240 and 298 sec). The 30‐day rates of major and minor bleeding, major adverse cardiovascular events (MACE), and net adverse clinical events (NACE; MACE or major bleeding) were determined.ResultsMortality at 30 days occurred in 2.2, 3.3, and 3.5% of patients in the low to high ACT tertiles, respectively (Ptrend = 0.22). Nor was the peak ACT significantly related to major bleeding, MACE or NACE. However, minor bleeding was increased in the highest ACT tertile (14.7% vs. 14.2% vs. 19.4%, Ptrend = 0.04). By multivariable analysis peak ACT was not significantly related to major bleeding, mortality, MACE, and NACE but was a significant independent predictor of minor bleeding (odds ratio = 1.027 [1.013, 1.042], P < 0.001, for each 10 sec increase in ACT).ConclusionsIn patients undergoing primary PCI for STEMI treated with UFH+GPI, the peak procedural ACT achieved does not have a substantial effect on major bleeding, mortality, or MACE, although lower peak ACT is associated with less minor bleeding. © 2012 Wiley Periodicals, Inc.
Successful high‐risk percutaneous coronary intervention with the use of minimal extracorporeal circulation system
Authors: Anastasiadis Kyriakos , Hadjimiltiades Stavros , Antonitsis Polychronis 
Published: April 2012

AbstractMinimal extracorporeal circulation (MECC) represents a contemporary system which integrates several advances in cardiopulmonary bypass technology in a single circuit. We challenged the efficacy of the MECC system to support the circulation in elective high‐risk percutaneous coronary intervention (PCI). A 78‐year‐old patient with complex coronary disease who would have been otherwise rejected for interventional therapy underwent PCI with rotablation on MECC support. The MECC system provided hemodynamic support at a flow of 1.8 L min−1 m−2 while perfusion pressure was kept at a minimum of 70 mm Hg. This allowed for successful angioplasty of the left main stem and a chronically occluded right coronary artery, which otherwise produced significant hemodynamic compromise. This case illustrates that mechanical circulatory support with the MECC system could provide a stable environment and a “safety net” for carrying out complex percutaneous coronary intervention in high‐risk patients. © 2012 Wiley Periodicals, Inc.
Predictors of an optimal clinical outcome with alcohol septal ablation for obstructive hypertrophic cardiomyopathy
Authors: Sorajja Paul , Binder Josepha , Nishimura Rick A. , Holmes David R. , Rihal Charanjit S. , Gersh Bernard J. , Bresnahan John F. , Ommen Steve R. 
Published: April 2012

AbstractBackgroundAlcohol septal ablation has emerged as a therapy for patients with obstructive hypertrophic cardiomyopathy (HCM). However, there are limited data on the predictors of success with the procedure.MethodsWe examined patient characteristics and cardiac morphology as well as procedural data on 166 HCM patients (mean age, 63 years; 43% men), who underwent ablation at Mayo Clinic. Patients were contacted to determine vital status and symptoms to assess the primary endpoint of survival free of death and severe symptoms (New York Heart Association, class III or IV dyspnea).ResultsThe strongest patient characteristics that predicted clinical success were older age, less severe left ventricular outflow tract gradient, lesser ventricular septal hypertrophy, and a smaller left anterior descending (LAD) diameter. Mitral valve geometry or ventricular septal morphology did not predict outcome. Patients with ≥3 characteristics (age ≥65 years, gradient <100 mmHg, septal hypertrophy ≤18 mm, LAD diameter <4.0 mm) had superior 4‐year survival free of death and severe symptoms (90.4%) in comparison to those with two characteristics (81.6%) and ≤1 characteristic (57.5%). Case volume with >50 patients was an independent predictor of survival free of severe symptoms. The volume of alcohol injected, number of arteries injected, or size of septal perforator artery were not predictive of clinical success.ConclusionsGreater case volume and selection for key patient and anatomic characteristics are associated with superior outcomes with alcohol septal ablation. © 2012 Wiley Periodicals, Inc.
Resolution of 2‐chloromandelic acid with (R)‐(+)‐N‐benzyl‐1‐phenylethylamine: chiral discrimination mechanism
Authors: Peng Yangfeng , He Quan , Rohani Sohrab , Jenkins Hilary 
Published: April 2012

AbstractDuring the resolution of 2‐chloromandelic acid with (R)‐(+)‐N‐benzyl‐1‐phenylethylamine, the crystals of the less soluble salt were grown, and their structure were determined and presented. The chiral discrimination mechanism was investigated by examining the weak intermolecular interactions (such as hydrogen bond, CH/π, and van der Waals interactions) and molecular packing mode in crystal structure of the less soluble diastereomeric salt. A one‐dimensional double‐chain hydrogen‐bonding network and a “lock‐and‐key” supramolecular packing mode are disclosed. The investigation demonstrates that hydrophobic layers with corrugated surfaces can fit into the grooves of one another to realize a compact packing, when the molecular structure of resolving agent is much larger than that of the racemate. This “lock‐and‐key” assembly is recognized to be another characteristic of molecular packing contributing to the chiral discrimination, in addition to the well‐known sandwich‐like packing by hydrophobic layers with planar boundary surfaces. Chirality 24:349–355, 2012. © 2012 Wiley Periodicals, Inc.
Novel Chiral Selector Based on Mefloquine – A Comparative NMR Study to Elucidate Intermolecular Interactions with Acidic Chiral Selectands
Authors: Kohout Michal , Kählig Hanspeter , Wolrab Denise , Roller Alexander , Lindner Wolfgang 
Published: April 2012

ABSTRACTThe synthesis, ab initio calculations, and a comparative nuclear magnetic resonance study of a novel chiral mefloquine‐based selector (SO) are presented. On a series of variously N‐acyl protected leucine selectands (SAs), a feasibility study of mefloquine carbamate as a basic chiral solvating agent, and potential fluorophilic high‐performance liquid chromatography selector has been undertaken and evaluated. An analogy is drawn between the new SO and tert‐butylcarbamoyl quinidine as a reference. Chirality 24:936–943, 2012. © 2012 Wiley Periodicals, Inc.
Hormone receptor status influences the locoregional benefit of trastuzumab in patients with nonmetastatic breast cancer
Authors: Kim Michelle M. , Dawood Shaheenah , Allen Pamela , Sahin Aysegul A. , Woodward Wendy A. , Smith Benjamin D. , Strom Eric A. , Hunt Kelly K. , Meric‐Bernstam Funda , Gonzalez‐Angulo Ana Maria , Buchholz Thomas A. 
Published: April 2012

AbstractBACKGROUND:Previous studies have shown that hormone receptor (HR) and human epidermal growth factor receptor 2 (HER2) status influence the outcome of locoregional treatments. However, the interrelationship of these factors with trastuzumab is unclear. In this study, the role of HR and HER2 status on the locoregional benefit of trastuzumab treatment was investigated in patients with nonmetastatic breast cancer.METHODS:Locoregional outcomes of 5683 women treated at The University of Texas MD Anderson Cancer Center from 2000 to 2008 for invasive breast cancer were analyzed using Kaplan‐Meier and Cox regression methods to compare 6 subgroups: HR‐positive (HR+)/HER2‐negative (HER2−), HR−/HER2− (triple‐negative), HR+/HER2+ with or without trastuzumab, and HR−/HER2+ with or without trastuzumab.RESULTS:Overall, locoregional recurrence (LRR) was 5% at 5 years among patients with HER2+ disease. Patients with HR+/HER2+ disease treated with trastuzumab had half the rate of LRR as patients who did not receive trastuzumab, whereas patients with HR−/HER2+ disease had similar rates of LRR regardless of trastuzumab treatment. On Cox regression analysis comparing LRR risk to the cohort with HR+/HER2− disease, only the HR+/HER2+ cohort treated with trastuzumab had similar LRR risk (hazard ratio = 1.24, 95% confidence interval = 0.56‐2.73, P = .591). All other subgroups, including the HR+/HER2+ cohort who did not receive trastuzumab, had significantly worse outcomes. LRR risk was highest among patients with triple‐negative disease (hazard ratio = 4.73, 95% confidence interval = 3.42‐6.54, P < .001).CONCLUSIONS:Among patients with HR+/HER2+ disease, treatment with trastuzumab reduces LRR risk to the more favorable outcome of patients with HR+/HER2− disease. In contrast, the increased LRR risk among patients with HR−/HER2+ disease remains despite treatment with trastuzumab. Additional locoregional strategies are needed in this subgroup of patients. Cancer 2012. © 2012 American Cancer Society.
Involvement of epidermal growth factor receptor overexpression in the promotion of breast cancer brain metastasis
Authors: Nie Fang , Yang Jian , Wen Song , An Yan‐Li , Ding Jie , Ju Sheng‐Hong , Zhao Zhen , Chen Hua‐Jun , Peng Xin‐Gui , Wong Stephen T. C. , Zhao Hong , Teng Gao‐Jun 
Published: April 2012

AbstractBACKGROUND:Brain‐metastatic breast cancer (BMBC) is increasing and poses a severe clinical problem because of the lack of effective treatments and because the underlying molecular mechanisms are largely unknown. Recent work has demonstrated that deregulation of epidermal growth factor receptor (EGFR) may correlate with BMBC progression. However, the exact contribution that EGFR makes to BMBC remains unclear.METHODS:The role of EGFR in BMBC was explored by serial analyses in a brain‐trophic clone of human MDA‐MB‐231 breast carcinoma cells (231‐BR cells). EGFR expression was inhibited by stable short‐hairpin RNA transfection or by the kinase inhibitor erlotinib, and it was activated by heparin‐binding epidermal growth factor‐like growth factor (HB‐EGF). Cell growth and invasion activities also were analyzed in vitro and in vivo.RESULTS:EGFR inhibition or activation strongly affected 231‐BR cell migration/invasion activities as assessed by an adhesion assay, a wound‐healing assay, a Boyden chamber invasion assay, and cytoskeleton staining. Also, EGFR inhibition significantly decreased brain metastases of 231‐BR cells in vivo. Surprisingly, changes to EGFR expression affected cell proliferation activities less significantly as determined by a 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay, an anchorage‐independent growth assay, and cell cycle analysis. Immunoblot analysis suggested that EGFR drives cells' invasiveness capability mainly through phosphoinositide 3‐kinase/protein kinase B and phospholipase C γ downstream pathways. In addition, EGFR was involved less in proliferation because of the insensitivity of the downstream mitogen‐activated protein kinase pathway.CONCLUSIONS:The current results indicated that EGFR plays more important roles in cell migration and invasion to the brain than in cell proliferation progression on 231‐BR cells, providing new evidence of the potential value of EGFR inhibition in treating BMBC. Cancer 2012. © 2012 American Cancer Society.
A 13 bit 10 MHz bandwidth MASH 3–2 Σ∆ modulator in 90 nm CMOS
Authors: Sohrabi Zahra , Yavari Mohammad 
Published: April 2012

ABSTRACTIn this paper, a new multi loop sigma‐delta (ΣΔ) modulator is proposed which employs one order redundant noise shaping in the first stage so the effect of the quantization noise leakage is minimized. Thus, analog circuit requirements are considerably relaxed compared to the conventional Multi‐stAge‐noise‐SHaping (MASH) structures. This enhancement makes the structure appropriate for low voltage and broadband applications. The proposed architecture is compared with traditional high‐order structures, and the advantages are demonstrated by both the analysis and behavioral system level simulations. As a prototype, the proposed MASH 3–2 sigma‐delta modulator is designed, and the detailed design procedure is presented from the system level to the circuit level in a 90 nm CMOS technology. Circuit level simulation results show that the modulator achieves a peak signal‐to‐noise and distortion ratio of 79.4 dB and 79 dB dynamic range over a 10 MHz bandwidth with a sampling frequency of 160 MHz. It consumes 35.4 mW power from a single 1 V supply. Copyright © 2012 John Wiley & Sons, Ltd.
Cryptococcal lymphadenitis in HIV: A chance diagnosis by FNAC
Authors: Bhuyan Pallavi , Pattnaik Kaumudee , Kar Asaranti , Brahma Rathish Chandra , Mahapatra Smita 
Published: April 2012

AbstractCryptococcosis is a life threatening, opportunistic fungal disease in human immunodeficiency virus‐infected individual. Lymph node involvement as a presenting feature in Cryptococcosis is not a common manifestation. A prompt diagnosis is of utmost importance in this disseminated form of cryptococcosis. There are very few reports, however, of cryptococcal lymphadenitis as a presenting feature. We report here a case of cryptococcal lymphadenitis that was diagnosed by fine‐needle aspiration cytology of the involved cervical lymph node. Diagn. Cytopathol. 2013. © 2012 Wiley Periodicals, Inc.