bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparation, mechanical properties, and in vitro biocompatibility of novel nanocomposites based on polyhexamethylene carbonate fumarate and nanohydroxyapatite
Authors: Sharifi Shahriar , Kamali Mehdi , Mohtaram Nima Khadem , Shokrgozar Mohammad Ali , Rabiee Sayed Mahmood , Atai Mohammad , Imani Mohammad , Mirzadeh Hamid 
Published: October 2009

AbstractIn the current study a new biodegradable nanocomposite based on poly hexamethylene carbonate fumarate (PHMCF) and nano‐sized hydroxyapatite (nano‐HA) has been developed. A silane coupling agent γ‐methacryloxypropyltrimethoxy silane, was used to achieve a good interfacial adhesion between nano‐HA and PHMCF matrix. PHMCF with different nano‐HA contents were characterized using dynamical mechanical thermal analysis (DMTA) and hardness test. The effect of frequency on storage modulus, glass transition temperature (Tg) and the damping were investigated. In vitro cytotoxicity and proliferation were performed using G292 cell lines by MTT assay. The addition of nano‐HA resulted in an increment on the storage modulus and decrement on the damping. Along with improvement in mechanical properties of composites, the addition of nano‐HA resulted in enhanced cell proliferation. Following these results, the newly developed nano‐PHMCF composite scaffold may be considered for bone tissue engineering applications. Copyright © 2009 John Wiley & Sons, Ltd.
Reduced CRYL1 expression in hepatocellular carcinoma confers cell growth advantages and correlates with adverse patient prognosis
Authors: Cheng Ibis K‐C , Ching Arthur K‐K , Chan Tsz‐Choi , Chan Anthony W‐H , Wong Chun‐Kwok , Choy Kwong‐Wai , Kwan Man , Lai Paul B‐S , Wong Nathalie 
Published: October 2009

AbstractHomozygous deletion screening has been widely utilized to define tumour suppressor genes (TSGs) in cancers. Although these biallelic deletions are infrequent, their identification has facilitated the discovery of many important TSGs. We have systematically examined the genome of hepatocellular carcinoma (HCC), a highly malignant tumour that is rapidly fatal, for the presence of homozygous deletions. Array‐CGH analysis on early passage of HCC cultures and cell lines led us to identify six homozygous deleted (HD) regions. A high concordance between array‐CGH and expression of HD genes was demonstrated, where crystallin Lambda1 (CRYL1; located on chromosome 13q12.11) displayed the most frequent down‐regulation. We found that reduced mRNA expression of CRYL1 was common in HCC tumours when compared with their adjacent non‐tumoural liver (p = 0.0097). Significant associations could also be drawn between repressed CRYL1 and advanced tumour staging, increased tumour size, and shorter disease‐free survival of patients (p < 0.037). Moreover, homozygous deletions on CRYL1 could be detected in 36% of HCC cases, where recurrent HDs were identified on exons 1, 5, and 8. Examination of other causal events suggested histone deacetylation and promoter hypermethylation to be likely inactivating mechanisms as well. Re‐expression of CRYL1 in the SK‐Hep1 cell line, where biallelic loss of CRYL1 was found, induced profound inhibition of cellular proliferation and cell growth (p < 0.0015). By Annexin V staining, CRYL1 restoration readily increased pro‐apoptotic cells with an induction of PARP cleavage. Flow cytometry further revealed that CRYL1 could prolong the G2–M phase, possibly through interruption of the Cdc2/cyclin B pathway. Given that regional chromosome 13q12–q14 loss is a causal genomic event in HCC tumourigenesis, our finding may have implications for identifying a novel TSG CRYL1 within this important locus. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Characterization of a WiT49 cell line derived orthotopic model of Wilms tumor
Authors: Li Mei‐Hong , Yamase Harold , Ferrer Fernando 
Published: October 2009

AbstractWilms tumor is the most common malignant renal tumor in children. However, to date no Wilms tumor mouse model is available due to the lack of Wilms tumor cell lines. Herein for the first time we report an orthotopic xenograft mouse model utilizing the recently described Wilms tumor cell line WiT49. It has a high tumor occurrence rate (85%) without metastasis. Hematoxylin and eosin staining showed it is subcapsular in location and mainly biphasic with stromal and epithelial components while blastemal component is unappreciable. This model provides the prerequisite for the screening and development of new anti‐tumor agents for Wilms tumor. Pediatr Blood Cancer 2010;54:316–318. © 2009 Wiley‐Liss, Inc.
Synthesis and curing behavior of macrocycle‐terminated poly(aryl ether ketone)s
Authors: Yue Xigui , Zhang Haibo , Liu Baijun , Wang Lifeng , Wang Guibin , Jiang Zhenhua 
Published: October 2009

AbstractBACKGROUND: The introduction of poly(ether ether ketone)‐based carbon‐fiber composites accelerated the application of poly(ether ether ketone)s in advanced composite materials. In order to improve the compatibility and processability with reinforced components, polymers with low melt viscosity are preferable.RESULTS: Novel fully aromatic macrocycle‐terminated poly(aryl ether ketone)s (MCPAEKs) were prepared by condensation of macrocyclic aryl ether ketone dimers containing hydroxyphenyl groups and fluorine end‐capped poly(aryl ether ketone) oligomers. Compared with liner poly(aryl ether ketone)s, MCPAEKs showed much lower melt viscosities at low temperature. In the presence of caesium fluoride, the crosslinking reaction of MCPAEKs afforded fully aromatic thermoset poly(aryl ether ketone)s by ring‐opening reaction.CONCLUSION: The MCPAEKs exhibited high thermal stability due to their wholly aromatic structures. After crosslinking, the glass transition temperatures and complex melt viscosities of the polymers were increased greatly. Although there was some residual cesium fluoride or phenoxides produced by ring‐opening reaction, the thermoset poly(aryl ether ketone)s obtained had good thermal stability with temperatures at 5% weight loss above 475 °C. Copyright © 2009 Society of Chemical Industry
Preserving the yeast proteome from sample degradation
Authors: Grassl Julia , Westbrook Jules A. , Robinson Aisling , Borén Mats , Dunn Michael J. , Clyne Rosemary K. 
Published: October 2009

AbstractSample degradation is a common problem in all types of proteomic analyses as it generates protein and peptide fragments that can interfere with analytical results. An important step in preventing such artefacts is to preserve the native, intact proteome as early as possible during sample preparation prior to proteomic analysis. Using the budding yeast Saccharomyces cerevisiae, we have evaluated the effects of trichloroacetic acid (TCA) and thermal treatments prior to protein extraction as a means to minimise proteolysis. TCA precipitation is commonly used to inactivate proteases; thermal stabilisation is used to heat samples to approximately 95°C to inactivate enzyme activity. The efficacy of these methods was also compared with that of protease inhibitors and lyophilisation. Sample integrity was assessed by 2‐D PAGE and a selection of spots was identified by MS/MS. The analysis showed that TCA or thermal treatment significantly reduced the degree of degradation and that these pre‐treatment protocols were more effective than treatment with either protease inhibitors or lyophilisation. This study establishes standardised sample preparation methods for the reproducible analysis of protein patterns by 2‐D PAGE in yeast, and may also be applicable to other proteomic analyses such as gel‐free‐based quantitation methods.
Analysis of Candida albicans plasma membrane proteome
Authors: Cabezón Virginia , Llama‐Palacios Arancha , Nombela César , Monteoliva Lucía , Gil Concha 
Published: October 2009

AbstractThe opportunistic human fungal pathogen Candida albicans causes a wide variety of infections including deep systemic syndromes. The C. albicans plasma membrane is an important interface in the host–pathogen relationship. The plasma membrane proteins mediate a variety of functions, including sensing and signalling to the external environment, in which the glycosylphosphatidylinositol (GPI)‐anchored membrane proteins play a crucial role. A subproteomic approach to obtain a global picture of the protein composition of the C. albicans plasma membrane was developed, and different strategies were tested in order to extract the largest number of yeast plasma membrane proteins and GPI‐anchored membrane proteins. These methods involved: (i) protoplast generation, (ii) mechanical disruption, (iii) ultracentrifugation in sucrose gradients, and (iv) Na2CO3 treatments. To isolate GPI‐anchored proteins two additional steps were performed: two‐phase separation and phosphatidylinositol‐phospholipase C treatment. After LC‐MS/MS analysis using both a MALDI‐TOF/TOF and a linear ion trap quadrupole, a total of 214 membrane proteins were identified, including 41 already described as plasma membrane proteins, 20 plasma membrane associated proteins, and 22 proteins with unknown membrane localisation. Bioinformatic analysis revealed that this set of C. albicans membrane proteins is highly enriched in proteins involved in biopolymer biosynthesis or transport processes. Furthermore, after phosphatidylinositol‐phospholipase C treatment, 12 GPI‐anchored membrane proteins were released and identified; most of them are associated with cell wall β‐glucan synthesis and maintenance or are virulence factors, such as phospholipases or aspartyl proteinases.
A new donor–acceptor double‐cable carbazole polymer with perylene bisimide pendant group: Synthesis, electrochemical, and photovoltaic properties
Authors: Koyuncu Sermet , Zafer Ceylan , Koyuncu Fatma Baycan , Aydin Banu , Can Mustafa , Sefer Emre , Ozdemir Eyup , Icli Siddik 
Published: October 2009

AbstractWe report here electrochemical synthesis of novel soluble donor–acceptor (D–A) polymer with suitably functionalized perylenetetracarboxylic diimide dye derivative covalently linked to carbazole moiety (Cbz‐PDI). The band gap, Eg was measured using UV–Vis spectroscopy and compared with that obtained by cyclic voltammetry (CV). Efficient intramolecular electron transfer from carbazole‐donor to perynediimide‐acceptor leads to remarkable fluorescence quenching of the perylene core. Furthermore, spectroelectrochemical property and surface morphology of the polymer film were investigated. Characteristic monoanion and dianion radical bands on the UV–Vis absorption spectra attributed to the electrochemical reduction of the neutral polymer were observed. During the reduction process, red color of the film turned into blue and violet, respectively. Finally, the photovoltaic performance of the D–A double‐cable polymer was checked and nearly 0.1% electrical conversion efficiency is obtained under simulated AM 1.5 solar light with 100 mW/cm2 radiation power. © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 6280–6291, 2009
Self‐assembly of well‐defined amphiphilic polymeric miktoarm stars, dendrons, and dendrimers in water: The effect of architecture
Authors: Lonsdale Daria E. , Whittaker Michael R. , Monteiro Michael J. 
Published: October 2009

AbstractFive polymeric architectures with a systematic increase in architectural complexity were synthesized by “click” reactions from a toolbox of functional linear polymers and small molecule linkers. The amphiphilic architectures ranged from a simple 3‐miktoarm star block copolymer to the more complex third generation dendrimer‐like block copolymer, consisting of polystyrene (PSTY) and polyacrylic acid (PAA). Micellization of these architectures in water at a pH of 7 under identical ionic strength gave spherical micelles ranging in size from 9 to 30 nm. Subsequent calculations of the PSTY core density, average surface area per PAA arm on the corona‐core interface, and the relative stretching of the PAA arms provided insights into the effect of architecture on the self‐assembly processes. A particular trend was observed that with increased architectural complexity the hydrodynamic diameter, radius of the core in the dry state and the aggregation number also increased with the exception of the third generation dendrimer. On the basis of these observations, we postulate that thermodynamic factors controlling self‐assembly were the entropic penalty of forming PSTY loops in the core counterbalanced by the reduction in repulsive forces through chain stretching. This results in a greater number of aggregating unimers and consequently larger micelle sizes. The junction points within the architecture also play an important role in controlling the self‐assembly process. The G3 dendrimer showed results contradictory to the aforementioned trend. We believe that the self‐assembly process of this architecture was dominated by the increased attractive forces due to stretching of the PSTY core chains to form a more compact core. © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 6292–6303, 2009
Single electron transfer‐living radical polymerization of methyl methacrylate in fluoroalcohol: Dual control over molecular weight and tacticity
Authors: Wang Wenxiang , Zhang Zhengbiao , Zhu Jian , Zhou Nianchen , Zhu Xiulin 
Published: October 2009

AbstractThe Cu(0)‐mediated single electron transfer‐living radical polymerization (SET‐LRP) of methyl methacrylate (MMA) using ethyl 2‐bromoisobutyrate (EBiB) as an initiator with Cu(0)/N,N,N′,N′′,N′′‐pentamethyldiethylenetriamine as a catalyst system in 1,1,1,3,3,3‐hexafluoro‐2‐propanol (HFIP) was studied. The polymerization showed some living features: the measured number‐average molecular weight (Mn,GPC) increased with monomer conversion and produced polymers with relatively low polydispersities. The increase of HFIP concentration improved the controllability over the polymerization with increased initiation efficiency and lowered polydispersity values. 1H NMR, MALDI‐TOF‐MS spectra, and chain extension reaction confirmed that the resultant polymer was end‐capped by EBiB species, and the polymer can be reactivated for chain extension. In contrast, in the cases of dimethyl sulfoxide or N,N‐dimethylformamide as reaction solvent, the polymerizations were uncontrolled. The different effects of the solvents on the polymerization indicated that the mechanism of SET‐LRP differed from that of atom transfer radical polymerization. Moreover, HFIP also facilitated the polymerization with control over stereoregularity of the polymers. Higher concentration of HFIP and lower reaction temperature produced higher syndiotactic ratio. The syndiotactic ratio can be reached to about 0.77 at 1/1.5 (v/v) of MMA/HFIP at −18 °C. In conclusion, using HFIP as SET‐LRP solvent, the dual control over the molecular weight and tacticity of PMMA was realized. © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 6316–6327, 2009
Psychometric properties of the Dutch version of the Mental Adjustment to Cancer scale in Dutch cancer patients
Authors: Braeken Anna P. B. M. , Kempen Gertrudis I. J. M. , Watson Maggie , Houben Ruud M. A. , Gils Francis C. J. M. v. , Lechner Lilian 
Published: October 2009

AbstractObjectives: The measurement of adjustment to cancer is relevant for research purposes and daily practice. In this study, the psychometric properties of the original five subscales and the two recently proposed summary scales of the Mental Adjustment to Cancer (MAC) scale were examined in Dutch cancer patients.Methods: Data from 289 cancer patients were assessed with the Dutch version of the MAC scale and the Hospital and Anxiety Depression scale (HADS); 259 patients completed the MAC scale for a second time.Results: In total, 85.5% of the participants completed the full MAC scale. The internal consistency of the five subscales and the summary scales were mostly similar to the original versions. The test–retest reliability of the Fighting Spirit, Helplessness/Hopelessness, Anxious Preoccupation, Summary Positive Adjustment and Summary Negative Adjustment subscales were moderate and the test–retest reliability of the Fatalism and Avoidance subscales were low. Correlations between the original and the two summary scales of the MAC scale and the depression and anxiety subscales of the HADS indicated good convergent validity. The structure of the five original subscales as well as the structure of the two proposed summary scales was adequate as shown by construct validity using confirmatory factor analyses.Conclusion: The Dutch version of the MAC scale is a feasible questionnaire and appeared to have comparable psychometric properties as demonstrated by studies in the UK. The psychometric properties of the summary scales and Fighting Spirit and Helplessness/Hopelessness subscales seem to be acceptable. This supports the cross‐national usefulness of the MAC scale. Copyright © 2009 John Wiley & Sons, Ltd.
The development of a cancer genetic‐specific measure of coping: the GRACE
Authors: Phelps C. , Bennett P. , Jones H. , Hood K. , Brain K. , Murray A. 
Published: October 2009

AbstractObjective: Generic measures of coping fail to capture the process of undergoing specific health processes such as cancer genetic risk assessment. The Genetic Risk Assessment Coping Evaluation (GRACE) has been developed to provide greater specificity of measurement.Method: Based upon previous research findings, the GRACE measures the degree of stress associated with 11 recognised sources of stress for individuals undergoing the early stages of cancer genetic risk assessment, and the use of up to eight coping strategies they may elicit. This paper reports preliminary data from the piloting of the GRACE within a randomised trial of a coping intervention.Results: Of the 265 participants who completed and returned their baseline questionnaire (prior to being informed of their level of genetic risk), 257 completed the GRACE. The most highly endorsed sources of stress involved concerns relating to family members, endorsed by over 60% of respondents, and concerns about how the participants would cope if found to be at increased risk (59%). Participants made use of multiple coping strategies across different sources of stress. The most frequently reported coping strategies were emotion‐focused, which may reflect the stage of the assessment process.Conclusion: The completion rates for the matrix and specificity of responses provided suggest that the GRACE may be an acceptable measurement tool. Further data collection and validation is ongoing. Copyright © 2009 John Wiley & Sons, Ltd.
Is emotional dissonance more prevalent in oncology care? Emotion work, burnout and coping
Authors: Kovács Mariann , Kovács Eszter , Hegedűs Katalin 
Published: October 2009

AbstractObjectives: Emotional burden on oncology care workers is considerable. These workers develop confidential relationship with the patient through interpersonal communication, which entails managing their own emotions as well as the emotions displayed by their patients, and it involves a great deal of emotion work. The objectives in our study were to assess the prevalence of burnout and emotional dissonance and to investigate the interrelationship among burnout, emotion work and coping in oncology care.Method: A cross‐sectional survey with anonymous questionnaires was conducted among oncology health care workers (N = 48) and non‐oncology health care workers (N = 151).Results: The comparison revealed differences primarily in emotion work and coping. Emotional dissonance as stress factor was more prevalent among oncology health care workers. Caregivers dealing with cancer patients felt that they have to display negative emotions less frequently, yet at the same time they frequently have to show understanding and express sympathy to the patient. When certain coping strategies were examined, we found that humour as potential resource in coping is used less frequently among oncology health care workers.Conclusion: In order to devise effective interventions to oncology personnel, we need to focus on the interaction between the carer and the cancer patient and have more evidence on emotional dissonance in oncology staff. Copyright © 2009 John Wiley & Sons, Ltd.
Experiences and concerns about ‘returning to work’ for women breast cancer survivors: a literature review
Authors: Tiedtke Corine , de Rijk Angelique , Dierckx de Casterlé Bernadette , Christiaens Marie‐Rose , Donceel Peter 
Published: October 2009

AbstractObjective: To explore how female breast cancer patients experience work incapacity during the treatment and return‐to‐work phases and how interactions between patients and stakeholders affect this experience.Method: Database search for full text articles published between January 1995 and January 2008 that focused on employed female breast cancer patients, factors related to work incapacity, and returning to work. Only results based on self‐report data were included. Studies focusing on treatment, financial factors, rate of return, or absence were excluded.Results: Six articles met the inclusion criteria. Women with breast cancer receive varied reactions but little advice about returning to work. Women were primarily concerned with disclosing the diagnosis to their employer and to relatives. Uncertainties about physical appearance, ability to work, and possible job loss affected the women's decisions about working during the treatment phase. After treatment, most women wanted to regain their ‘normal life’, but concentration and arm or fatigue problems potentially interfered. Although supportive work environments were helpful, the individual needs of women differed. Employers and employees need to find a balance in defining accommodating work. Many women received favourable support, but some reported feeling discriminated against. Many women re‐evaluated the role of work in their lives after being confronted with breast cancer.Conclusion: Work adjustments could help women to keep their jobs during illness and recovery. To resolve women's concerns about returning to work, employers, physicians, and insurance institutions should consider increasing and improving communication with breast cancer patients and playing a more active and supportive role. Copyright © 2009 John Wiley & Sons, Ltd.
Use of intrapulmonary percussive ventilation (IPV) in the management of pulmonary complications of an infant with osteogenesis imperfecta
Authors: Nino Gustavo , McNally Paul , Miske Laura J. , Hickey Eileen , Panitch Howard B. 
Published: October 2009

AbstractOsteogenesis imperfecta (OI) is a genetic disorder characterized by abnormal collagen formation and short stature. These patients present with frequent vertebral, rib, and long bone fractures. There are many respiratory complications associated with OI including pneumonia, the most common cause of mortality in the severe forms of the disease. We present a case of an infant with OI (type III/IV) and significant tracheobronchomalacia who had required multiple hospitalizations for recurrent atelectasis and respiratory failure in the setting of acute respiratory infections. External chest percussion and vibration were avoided because of the risk of rib fractures. intrapulmonary percussive ventilation (IPV) was initiated during an acute illness with good effect, and continued successfully after discharge from hospital. We conclude that IPV represents a safe and effective alternative to airway clearance in infants with OI. Pediatr Pulmonol. 2009; 44:1151–1154. ©2009 Wiley‐Liss, Inc.