bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Potential Anticancer Potency of Kolaviron on Colorectal Adenocarcinoma (Caco-2) Cells
Authors: Althagafi Hussam A. 
Published: June 2024

Background:
Globally, colorectal cancer (CRC) is categorized as the third type of cancer associated
with mortalities. Chemotherapeutic drugs such as cisplatin can be used to treat cancer-affected patients. However,
several adverse effects are associated with its application. This motivated the researchers to search for alternatives
that are more efficient and have fewer undesirable effects. Kolaviron is a bioflavonoid that has been reported to
have antioxidant and anti-inflammatory properties.

Aim:
This study aimed to compare the anticancer effects of kolaviron and cisplatin on Caco-2 cells. The IC50 of
kolaviron and cisplatin were calculated, and redox status, apoptotic-related proteins and the cell cycle were also
examined.

Methods:
Caco-2 cells were treated with kolaviron (⅟3 and ½ of IC50 dose) and cisplatin (IC50 dose) for 24 h and
48 h. Cell viability was assessed using the MTT protocol. Redox status and apoptotic-related proteins, in addition
to the cell cycle, were examined.

Results:
The MTT assay showed the IC50 of kolaviron is 9.49 μg/mL, and that of cisplatin is 2.71 μg/ml against
Caco-2 cells. Further, both doses of kolaviron significantly increased the leakage of lactate dehydrogenase
(LDH), the production of reactive oxygen species (ROS), and lipoperoxidation (LPO), besides decreasing the
antioxidant potency of tumor cells as revealed by the diminished reduced glutathione (GSH). At the molecular
level, a significant increase in the levels of p53, cytochrome c, Bax, and caspase 3 was recorded, coupled with a
decrease in the level of Bcl2, after treating the Caco-2 cells with kolaviron and cisplatin. Furthermore, kolaviron
demonstrated asserted more effects on apoptosis and increased cell percentage in the subG1 phase. In addition, a
notable decrease in the expression of proliferating cell nuclear antigen (PCNA) and cyclin D1 is associated with
an increase in the expression of tumor protein P53 (TP53) in kolaviron-treated Caco-2 cells cancerous cells.

Conclusion:
Conclusively, these data suggest that kolaviron has a potential antitumor capacity against colorectal
cancer via multiple pathways, including enhancement of ROS production, redox status, p53 pathway, and apoptosis.
Therefore, this study authenticated the capability of kolaviron as a valuable chemotherapeutic agent.
A Sustainable Revolution in Sisal Fiber with Enhanced Mechanical Properties of Concrete
Authors: Selvam Vijayan , Muniyandi Tholkapiyan , Jaya Ramadhansyah Putra 
Published: June 2024

Introduction
This study provides a comprehensive comparative analysis of conventional concrete (CC) and Sisal Fiber Concrete (SFC) and incorporates sisal fiber into a concrete blend for the M25 grade concrete mix.

Methods
In order to evaluate the efficacy of both variations of concrete, mechanical and durability parameters were examined. As compared to CC, the results indicated that SFC had a substantially greater compressive strength. The average compressive strength of SFC at the 28-day was 29.47 N/mm2, which signified a significant incremental percentage growth of 9.58% in comparison to CC. In the same way, SFC exhibited an exceptional flexural strength, as evidenced by its mean value of 7.81 N/mm2, which represented a significant 34.42% improvement in comparison to CC. The Bayesian factor independent sample test yielded a t-test value of 12.495 for compressive strength, accompanied by a p-value below 0.001. These results suggest that the observed difference was statistically significant. Conversely, a t-test value of 19.380 and a p-value below 0.001 were produced by the Bayesian factor independent sample test for flexural strength, both of which further supported the existence of a significant difference. The mean disparity in compressive strength between CC and SFC was 5.1522 N/mm2, with a 95% confidence interval encompassing values between 4.2856 and 6.0188 N/mm2. In a similar manner, the mean discrepancy in flexural strength was 2.0000 N/mm2, accompanied by a 95% confidence interval that varied between 1.7831 and 2.2169 N/mm2.

Results
The obtained results provide further evidence that SFC is stronger than CC in both compressive and flexural strength, which is consistent with the results obtained from the frequentist statistical analysis.

Conclusion
With its eco-friendly properties, sisal fiber concrete could indeed play a significant role in the future of sustainable construction.
The Effect of Using Jute on the Compressive Strength of Concrete
Authors: Shrestha Ram Krishna , Alagirisamy Mukil , Dangol Purushottam , Shakya Manjip , Kawan Chandra Kiran , Giri Om Prakash 
Published: June 2024

Background
Concrete comprises cement, fine aggregates, coarse aggregates, and water, making it the most widespread construction material globally. However, while producing cement as an important component of concrete, it generates significant CO2 emissions, worsening global warming and environmental challenges worldwide.

Objective
This study aims to investigate the impact of incorporating jute fiber into concrete mixes of varying proportions to enhance compressive strength.

Methods
Concrete mixes of three grades were prepared, with varying percentages of jute (0.5%, 1.0%, 1.5%, and 2%) by weight of cement. Compressive strength tests were conducted at 7 and 28 days of curing.

Results
It shows notable enhancements in strength, improvement of 15.07 % (for Nominal mix 1:1:2) and 20.29% (for Nominal mix 1:1.5:3 ) is at the addtion of 1% jute but 6.72% (for Nominal mix 1:2:4) is at addtion of 2% jute mix.

Conclusion
Findings suggest that adding jute fiber, particularly with higher cement content, significantly enhances concrete strength, emphasizing its potential as a reinforcing material for sustainable construction.
Metabolites of Culinary-medicinal Mushroom Hericium erinaceus Showed Anti-neuroinflammatory Activity in BV2 Cells and Anti-H2O2-induced Oxidative Stress Activity in SH-SY5Y Cells
Authors: Wei Jing , Lin Chao , Wang Zhen-Xin , Zhang Yi-Lin , Li Shi-Xi , Hu Xuan-Sheng , Hui He-Ping , Wang Zhe , Zhao Yi-Qi , Wang Xue-Jun , Qi Jianzhao 
Published: June 2024

Background:
Hericium erinaceus is a versatile mushroom with potential therapeutic benefits.
A variety of bioactive compounds have been found in this mushroom that have anti-ulcer, antiinflammatory,
immunomodulatory, and blood sugar and lipid regulating effects.

Objectives:
The aim is to isolate and characterize compounds from the rice medium of H. erinaceus
and evaluate their potential therapeutic effects on oxidative stress-associated diseases.

Methods:
Fifteen compounds were isolated and characterized using NMR spectra and ESI-MS. The
effects of compounds 3 and 7 on LPS-induced nitric oxide (NO) generation in BV2 cells and H2O2-
induced cytotoxicity in SH-SY5Y cells were investigated. The regulation of nitric oxide production
and apoptosis-associated proteins Bcl-2 and Bax were also examined.

Results:
Compounds 3 and 7 exhibited potent inhibitory effects on LPS-induced NO generation in
microglial cells and significant resistance to H2O2-induced cytotoxicity in neuronal cells. Molecular
docking simulations demonstrated the interaction of compounds 3 and 7 with iNOS and Bax, respectively.
Western blot analysis revealed that 7 inhibited NO production and regulated the expression of
Bax and Bcl-2, suggesting a protective effect against H2O2-induced apoptosis.

Conclusion:
Compounds 3 and 7 isolated from H. erinaceus are potential therapeutic candidates for
oxidative stress-associated diseases, possibly through their regulation of nitric oxide production and
apoptosis-related proteins. Further investigations are warranted to elucidate their mechanisms of
action and therapeutic potential.
In-vitro Study of HIV-derived Reverse Transcriptase Inhibition
Authors: Ibne Shoukani Hussan , Kubra Khudija tul , Ahmad Bashir 
Published: June 2024

Introduction:
HIV utilizes a reverse transcriptase (RT) enzyme to convert the HIVRNA
into DNA. Inhibition of the reverse transcription mechanism of HIV-RT may serve as a
potential therapeutic approach to impede the proliferation of HIV in those who are infected.
Non-nucleoside reverse transcriptase inhibitors (NNRTIs) are a type of medication that directly
and non-competitively bind to the allosteric site of HIV-RT, inhibiting its polymerase activity.

Aim:
This study was aimed at the synthesis of hydrazine derivatives and their evaluation for
HIV- reverse transcriptase inhibition using RT-qPCR-based assay.

Objective:
The objective of this study was to determine the HIV- reverse transcriptase inhibition
using chemical compounds as non-nucleoside reverse transcriptase inhibitors in RT-qPCR.

Methods:
This study involved the synthesis of five distinct hydrazine derivatives, which were
subsequently tested for their capacity to inhibit HIV-RNA polymerization by targeting HIVderived
reverse transcriptase. For the determination of the study assay, commercially available
HIV-RT was subjected to treatment with derivatives and utilized in an RT-qPCR experiment to
determine the activity or inhibitory effects of HIV-RT for HIV-RNA polymerization.

Results:
The in-vitro assay results demonstrated a reduction in viral load due to suppression of
reverse transcriptase activity when compared to the pre-quantified values obtained from untreated
RT. Among the five compounds, 4-N, N-dimethylamino benzaldehyde hydrazine
(C18H22N4) had the highest ability to suppress HIV-RT. This molecule reduced HIV-RNA reverse
transcription by more than 90% during RT-qPCR, which is a novel and promising strategy.

Conclusion:
N, N-dimethylamino benzaldehyde hydrazine (C18H22N4) can suppress the activity
of HIV-RT, and this effect becomes more pronounced as the concentration of the compound
increases.
Kajian Struktur Ruang Kecamatan Serengan Kota Surakarta
Authors: Ma'sum Moh. Ali 
Published: June 2024

Kecamatan Serengan sebagai salah satu kecamatan dengan penerapan sistem perkotaan di Kota Surakarta yang sudah terus mengalami perkembangan. Struktur ruang merupakan tatanan dari pusat pemukiman serta sistem sarana prasarana yang berguna dalam mendukung kegiatan ekonomi dan sosial masyarakat dimana secara hierarki mempunyai hubungan fungsional. Karena hal tersebut maka penulis mengkaji penelitian ini dengan tujuan untuk mengetahui struktur ruang Kecamatan Serengan Kota Surakarta. Penulis menggunakan jenis penelitian deskriptif dengan menerapkan pendekatan kuantitatif serta variabel penelitian ini berupa sarana prasarana yaitu fasilitas pelayanan masyarakat dan jaringan jalan. Hasil penelitian menunjukankan bentuk struktur kecamatan Serengan mendekati konsep pola konsentris, dimana pusat perkembangan dan pertumbuhan berada di tengah-tengah kecamatan Serengan selain itu model struktur ruangnya yaitu multi nodal karena terdiri dari pusat, sub pusat serta pusat lainya.
Model Regresi Spasial untuk Pendugaan Intrusi Air Laut Berdasarkan Relasi Pengukuran Salinitas terhadap Vegetation Soil Salinity Index
Authors: Dermawan Ananda , Ghazali Mochamad Firman , Salsabila Choirunnisa , Zahra Lauditta , Aulia Mila , Suastini Ni Made Mega Melliana 
Published: June 2024

Intrusi air laut di wilayah pesisir sering menjadi permasalahan bagi lingkungan sekitarnya sehingga perlu dilakukan upaya untuk mengidentifikasi keberadaan intrusi tersebut. Hal ini dapat dinilai menggunakan teknologi penginderaan jauh. penelitian ini dilakukan dengan menganalisis Citra Satelit Sentinel-2, data salinitas dari pengukuran lapangan, dan perhitungan statistik. Salinitas tanah akan diperkirakan menggunakan vegetation soil salinity index (VSSI). Penelitian ini mengintegrasikan data pengukuran salinitas lapangan dengan VSSI untuk membangun model persamaam regresi linear dan nonlinear. Ada tiga model regresi yang digunakan yaitu regresi linear, regresi eksponensial, dan regesi polynomial. Analisis ketiga model tersebut menunjukkan bahwa regersi polynomial merupakan model yang paling sesuai untuk estimasi salinitas berdasarkan VSSI dengan nilai koefisien determinasi (R2) 11,04%. Hasil estimasi salinitas berdasarkan VSSI menggunakan model polynomial memiliki koreasi yang cukup baik terhadap observasi salinitas dengan nilai koefisien determinasi (R2) 42,49%, sedangkan validasi estimasi tersebut memiliki akurasi cukup tinggi dengan nilai RMSE 0,0013% dan MAE 0,0011%. Kata kunci : Intrusi air laut, Salinitas, Analisis regresi, Sentinel-2, VSSI
Social Media Promotion of Health Tests With Potential for Overdiagnosis or Overuse: Protocol for a Content Analysis
Authors: Nickel Brooke , Heiss Raffael , Shih Patti , Gram Emma Grundtvig , Copp Tessa , Taba Melody , Moynihan Ray , Zadro Joshua 
Published: June 2024

Background
In recent years, social media have emerged as important spaces for commercial marketing of health tests, which can be used for the screening and diagnosis of otherwise generally healthy people. However, little is known about how health tests are promoted on social media, whether the information provided is accurate and balanced, and if there is transparency around conflicts of interest.

Objective
This study aims to understand and quantify how social media is being used to discuss or promote health tests with the potential for overdiagnosis or overuse to generally healthy people.

Methods
Content analysis of social media posts on the anti-Mullerian hormone test, whole-body magnetic resonance imaging scan, multicancer early detection, testosterone test, and gut microbe test from influential international social media accounts on Instagram and TikTok. The 5 tests have been identified as having the following criteria: (1) there are evidence-based concerns about overdiagnosis or overuse, (2) there is evidence or concerns that the results of tests do not lead to improved health outcomes for generally healthy people and may cause harm or waste, and (3) the tests are being promoted on social media to generally healthy people. English language text-only posts, images, infographics, articles, recorded videos including reels, and audio-only posts are included. Posts from accounts with <1000 followers as well as stories, live videos, and non-English posts are excluded. Using keywords related to the test, the top posts were searched and screened until there were 100 eligible posts from each platform for each test (total of 1000 posts). Data from the caption, video, and on-screen text are being summarized and extracted into a Microsoft Excel (Microsoft Corporation) spreadsheet and included in the analysis. The analysis will take a combined inductive approach when generating key themes and a deductive approach using a prespecified framework. Quantitative data will be analyzed in Stata SE (version 18.0; Stata Corp).

Results
Data on Instagram and TikTok have been searched and screened. Analysis has now commenced. The findings will be disseminated via publications in peer-reviewed international medical journals and will also be presented at national and international conferences in late 2024 and 2025.

Conclusions
This study will contribute to the limited evidence base on the nature of the relationship between social media and the problems of overdiagnosis and overuse of health care services. This understanding is essential to develop strategies to mitigate potential harm and plan solutions, with the aim of helping to protect members of the public from being marketed low-value tests, becoming patients unnecessarily, and taking resources away from genuine needs within the health system.

International Registered Report Identifier (IRRID)
DERR1-10.2196/56899
Low-Grade Ilmenite Leaching Kinetics Using Hydrochloric Acid: RSM and SCM Approaches
Authors: Supriyatna Yayat Iman , Prasetya Agus , Astuti Widi , Sumardi Slamet , Natalia Priskila , Adythia Dicky Marsa , Petrus Himawan Tri Bayu Murti 
Published: June 2024

Minerals containing TiO2 are common in Indonesia, such as ilmenite in iron sand deposits scattered along the country's coasts. Ilmenite is an important source of titanium. One method for making TiO2 from ilmenite is by solubilizing both the Fe and Ti elements in HCl and then immediately hydrolyze the Ti. The leaching of low-grade ilmenite (ground to 0.177-0.149 mm) is studied kinetically by HCl in a stirred reactor. The research was conducted using the caustic fusion method followed by HCl leaching. The leaching reaction kinetics at the optimum conditions are analyzed using response surface methodology (RSM) with a second-order polynomial equation model and SSE with the shrinking core model (SCM). The results showed that HCl concentration and leaching time were directly proportional to the leached titanium concentration. In contrast, the leaching temperature was inversely proportional. The optimum operating conditions were obtained at a temperature of 30 °C, 9 M HCl, and 120 min of leaching time. The shrinking core model is a better representation of the kinetics than RSM with a second-order polynomial equation model. Based on SCM, the rate of the leaching reaction of titanium from low-grade ilmenite is controlled by diffusion through the ash layer.
Characterization and Application of Natural Photosensitizer and Poly(vinylidene Fluoride) Nanofiber Membranes-Based Electrolytes in DSSC
Authors: Zakiyah Nafisatus , Kusumawati Nita , Setiarso Pirim , Muslim Supari , A'yun Qurrota , Putri Marinda Mayliansarisyah 
Published: June 2024

This comprehensive research has explored the potential of enhancing dye-sensitized solar cells (DSSC) by harnessing environmentally friendly natural dyes, such as chlorophyll pigments from pandanus (664.1 nm) and papaya leaves (664.0 nm), as well as betacyanin pigments from sappan-mangosteen (536.2 nm). Electrochemical analyses elucidated the energy band gaps, revealing a hierarchy with the smallest band gap observed for papaya leaves (1.387 eV), followed closely by sappan-mangosteen (1.389 eV) and pandan leaves (1.396 eV). This research effectively addressed the persistent issue of electrolyte leakage in DSSC development by introducing a polymer electrolyte derived from polyvinylidene fluoride (PVDF) through electrospinning and phase inversion techniques. SEM characterization results and thermogravimetric analysis underscored the superior characteristics and high thermal stability of the PVDF nanofiber polymer for DSSC applications. The study's pivotal findings underscore the remarkable DSSC performance achieved with chlorophyll pigment from papaya leaves, reaching 1.31% efficiency without a polymer electrolyte. Moreover, the sappan-mangosteen dye emerged as a promising contender with the highest efficiency values when applied with polymer electrolyte, recording rates of 1.17% for PVDF NF and 0.95% for PVDF, which are notably comparable to the efficiency of liquid electrolyte at 1.26%.
Chromatographic and Spectrophotometric Determination of Clindamycin in Pharmaceutical Products
Authors: Al-Momani Idrees Faleh , Al-Kilani Lana Mohammad Zaid 
Published: June 2024

Accurate, precise, and reliable chromatographic and spectrophotometric methods were developed for determining clindamycin (CLD) in pharmaceutical formulations. The spectrophotometric method was adopted for flow injection analysis (FIA). The method is based on the online oxidation of CLD and measuring the absorbance of the resulting product using a flow cell at 605 nm. Experimental conditions, including FIA variables and reaction conditions, were optimized. The chromatographic separation was achieved using a C8 column and an isocratic mobile phase. The composition of the mobile phase selected for the analysis consists of a mixture of phosphate buffer (50%), MeOH (35%), and ACN (15%), adjusted to a pH of 3.47 by phosphoric acid. The eluent was monitored with a UV detector at 205 nm. The linearity range was 10–200 and 50–800 µg/mL for the FIA and HPLC, respectively. The applicability of the FIA and HPLC methods was validated by analyzing CLD in synthetic and commercial pharmaceutical products. No significant interferences were observed from the common excipients usually used in commercial formulations.
Analisis Penentuan Prioritas Pengembangan Unsur Pendukung Desa Wisata di Dewa Leuwilaja, Kecamatan Sindangwangi, Kabupaten Majalengka
Authors: Sri Rum Giyarsih  , Evi Wulandari  
Published: June 2024

Seiring dengan berkembangnya zaman, sektor pariwisata khususnya desa wisata semakin berkembang dan menjadi bintang baru bagi perekonomian suatu wilayah. Desa Leuwilaja yang berada di Kecamatan Sindangwangi, Kabupaten Majalengka, Provinsi Jawa Barat memiliki potensi sumber daya ekonomi lokal berupa sentra kerajinan rotan yang dapat dikembangkan sebagai desa wisata rotan. Pemerintah desa maupun masyarakat memiliki keinginan untuk mengembangan desa wisata rotan sebagai wadah perekonomian masyarakat. Perlu adanya rencana program prioritas utama dalam pengembangan desa wisata untuk memudahkan stakeholder, baik pemerintah maupun masyarakat dalam melakukan pengembangan desa wisata. Analisis ini menjadi disusun sebagai bentuk pengabdian kepada masyarakat melalui kegiatan KKN-PPM UGM 2023 dengan tujuan untuk membantu dalam mengidentifikasi unsur pendukung dan strategi yang dapat diterapkan untuk mengoptimalkan potensi yang ada untuk pengembangan desa wisata. Analisis ini menggunakan pendekatan deskriptif kualitatif dengan metode pengumpulan data berupa wawancara, dokumentasi, observasi lapangan, dan dilanjutkan dengan pemetaan usaha rotan untuk mendukung hasil observasi lapangan. Sedangkan teknik analisis data yang dilakukan adalah analisis SWOT untuk menentukan strategi pengembangan desa wisata. Hasil penelitian menunjukkan bahwa unsur pendukung wisata yang berada di Desa Leuwilaja, baik dari aspek amenitas dan aksesibilitas sudah cukup optimal dalam mendukung adanya desa wisata, tetapi untuk kelembagan masih perlu ditingkatkan kembali karena masih kurangnya pengelolaan potensi wisata. Hal yang menjadi prioritas untuk dikembangkan terlebih dahulu guna mendukung pengembangan desa wisata di Desa Leuwilaja adalah mengembangkan atraksi baru, mengembangkan skill sumber daya manusia dalam membuat inovasi baru, mengoptimalkan fasilitas umum, pengembangan infrastruktur dan spatial planning, dan meningkatkan kerjasama dengan berbagai pihak luar.