bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bacterial dynamics of the plastisphere microbiome exposed to sub-lethal antibiotic pollution
Authors: Joannard Brune , Sanchez-Cid Concepcion 
Published: May 2024

Abstract
Background
Antibiotics and microplastics are two major aquatic pollutants that have been associated to antibiotic resistance selection in the environment and are considered a risk to human health. However, little is known about the interaction of these pollutants at environmental concentrations and the response of the microbial communities in the plastisphere to sub-lethal antibiotic pollution. Here, we describe the bacterial dynamics underlying this response in surface water bacteria at the community, resistome and mobilome level using a combination of methods (next-generation sequencing and qPCR), sequencing targets (16S rRNA gene, pre-clinical and clinical class 1 integron cassettes and metagenomes), technologies (short and long read sequencing), and assembly approaches (non-assembled reads, genome assembly, bacteriophage and plasmid assembly).

Results
Our results show a shift in the microbial community response to antibiotics in the plastisphere microbiome compared to surface water communities and describe the bacterial subpopulations that respond differently to antibiotic and microplastic pollution. The plastisphere showed an increased tolerance to antibiotics and selected different antibiotic resistance bacteria (ARB) and antibiotic resistance genes (ARGs). Several metagenome assembled genomes (MAGs) derived from the antibiotic-exposed plastisphere contained ARGs, virulence factors, and genes involved in plasmid conjugation. These include Comamonas, Chryseobacterium, the opportunistic pathogen Stenotrophomonas maltophilia, and other MAGs belonging to genera that have been associated to human infections, such as Achromobacter. The abundance of the integron-associated ciprofloxacin resistance gene aac(6’)-Ib-cr increased under ciprofloxacin exposure in both freshwater microbial communities and in the plastisphere. Regarding the antibiotic mobilome, although no significant changes in ARG load in class 1 integrons and plasmids were observed in polluted samples, we identified three ARG-containing viral contigs that were integrated into MAGs as prophages.

Conclusions
This study illustrates how the selective nature of the plastisphere influences bacterial response to antibiotics at sub-lethal selective pressure. The microbial changes identified here help define the selective role of the plastisphere and its impact on the maintenance of environmental antibiotic resistance in combination with other anthropogenic pollutants. This research highlights the need to evaluate the impact of aquatic pollutants in environmental microbial communities using complex scenarios with combined stresses.
Sex differences in electrolyte abnormalities indicating refeeding syndrome risk among hospitalized adolescents and young adults with eating disorders
Authors: Nagata Jason M. , Nguyen Anthony , Vargas Ruben , Downey Amanda E. , Chaphekar Anita V. , Ganson Kyle T. , Buckelew Sara M. , Garber Andrea K. 
Published: May 2024

Abstract
Background
Refeeding syndrome is the gravest possible medical complication in malnourished patients undergoing refeeding in the hospital. We previously reported that males with malnutrition secondary to eating disorders required more calories and had longer hospital stays than females; however, sex differences in electrolyte abnormalities indicating refeeding syndrome risk remain unknown. The objective of this study was to assess differences in electrolyte abnormalities indicating refeeding syndrome risk among male and female adolescents and young adults with eating disorders hospitalized for medical instability.

Methods
We retrospectively reviewed the electronic medical records of 558 patients aged 9–25 years admitted to the University of California, San Francisco Eating Disorders Program for medical instability between May 2012 and August 2020. Serum was drawn per standard of care between 5 and 7 am each morning and electrolyte abnormalities indicating refeeding syndrome risk were defined as: hypophosphatemia (< 3.0 mg/dL), hypokalemia (< 3.5 mEq/L), and hypomagnesemia (< 1.8 mg/dL). Logistic regression was used to assess factors associated with electrolyte abnormalities indicating refeeding syndrome risk.

Results
Participants included 86 (15.4%) males and 472 (84.6%) females, mean (SD) age 15.5 (2.8) years. Rates of refeeding hypophosphatemia (3.5%), hypokalemia (8.1%), and hypomagnesemia (11.6%) in males hospitalized with eating disorders were low, with no statistically significant differences from females. Older age was associated with higher odds of refeeding hypophosphatemia and hypomagnesemia. Lower percent median body mass index and greater weight suppression at admission were associated with higher odds of refeeding hypophosphatemia.

Conclusions
Rates of electrolyte abnormalities indicating refeeding syndrome risk were low in males hospitalized for eating disorders and rates did not significantly differ from females. Together with our finding that males have higher caloric requirements and longer hospital length of stay, the finding that electrolyte abnormalities indicating refeeding syndrome risk were not greater in males than females supports future research to evaluate the safety and efficacy of higher calorie and/or faster advancing refeeding protocols for males.
Changes in intestinal microbiota, immunity and metabolism caused by mixed Lactiplantibacillus plantarum and Bacillus subtilis-fermented feed in Bamei pigs
Authors: Wang Lei , Chen Jun , Zhang Jianbo , Xu Fafang , Luo Xuan , Pang Huili , Zhang Miao , Duan Yaoke , Cai Yimin , Wu Guofang , Tan Zhongfang 
Published: May 2024

Abstract
Background
The Chinese pig breed Bamei faces numerous challenges, such as antibiotic abuse, feed shortages, weaning stress, low immunity and disease resistance after weaning. Probiotic-fermented feed is an ideal profile that can improve the intestinal microbiota, promote the digestion and absorption of nutrients, and improve immunity. However, the combined effect of long-term intake of probiotic-fermented feeds on the intestinal microbiota, intestinal metabolic profiles, and immunity in pigs is not well understood. Here, we investigated the effects of feeding basal feed, Lactiplantibacillus-fermented feed, Bacillus subtilis-fermented feed, mixed-fermented feed, and antibiotic-added feed for 100 days on the gut microbiota, immunity, and metabolism of Bamei pigs after feeding five different fermented feeds by using 16S rDNA high-throughput sequencing, enzyme-linked immunoassay, and untargeted metabolomics, respectively.

Results
16S rDNA sequencing revealed that after the piglets were fed five different feeds for 50 days, the structure of the intestinal microbiota of the Bamei pigs was significantly altered, and feeding the mixed Lactiplantibacillus (L.) plantarum and Bacillus (B.) subtilis-fermented feed not only increased the α-diversity of the intestinal microbiota and the relative abundance of Lactobacillus, but also suppressed the growth of the conditional pathogens, Clostridium and Streptococcus. The Sobs and Shannon indices were significantly lower (p < 0.05) on Day 10 in Group A, which was fed feed supplemented with antibiotics. Feeding mixed-fermented feed not only significantly increased the production of anti-inflammatory cytokines, but also significantly decreased the production of several proinflammatory cytokines and inhibited the TLR4/MyD88/NF-κB inflammatory-related signaling pathway (p < 0.05), even more so than antibiotics. The results of untargeted metabolomics showed that feeding mixed-fermented feed improved the metabolism of Bamei pigs by increasing the content of narceine and alpha-cephalin; promoting bile secretion; and facilitating the synthesis of phenylalanine, tyrosine, and steroid hormones. ATP-binding cassette (ABC) transporters were significantly enriched in the antibiotic group.

Conclusion
The mixed L. plantarum QP28-1a and B. subtilis QB8a-fermented feed not only improved the intestinal microbiota structure and metabolic profiles and regulated the metabolic pathways of tryptophan, phenylalanine, and steroid hormone biosynthesis, but also improved the immunity of Bamei pigs. This research provides an ideal, healthful, and environmentally sustainable approach for Bamei pig breeding and conservation.

Graphical Abstract
Vice and Immoral Spaces
Authors: Martin Annalisa 
Published: May 2024

Abstract
In the aftermath of the Second World War, local authorities across West Germany implemented Sperrbezirke, or restricted areas for prostitution. These restricted areas became a central element of Germany’s system of managing commercial sex. This article considers both the legacies of former systems of state-regulated prostitution in the development of Sperrbezirke and regional variations in restricted-area regulations since the 1960s. It examines their relation to red-light districts through regulations on brothels and tolerance zones, as well as the common associations of Sperrbezirke with vice in popular culture. The article then uses prostitutes’ responses to restricted-area regulations to assess their impact in practice.
Digital vs. Traditional Advertising and the Recognition of Brand Intangible Assets
Authors: Song Scarlett Xiaotong 
Published: May 2024

This paper examines how different advertising categories are associated with attributes of brand asset recognition arising in the context of acquisitions. Prior research documents that aggregate advertising is positively associated with firm sales and brand values, but it fails to consider major recent shifts in advertising from traditional channels (such as TV) to digital advertising (such as paid search and online display). Using proprietary data, I decompose advertising expenditures into the categories of traditional, online display, and paid search. Consistent with expectations, results confirm that target firms’ traditional and online display advertising exhibit a higher likelihood of brand asset recognition and higher recognized brand values as compared with paid search advertising. Confirming the economic substance of the recognized brands, additional results reveal positive investor equity market reactions to brand value, which are driven by firms investing more in traditional and online display advertising. Overall, these results confirm that certain advertising channels exhibit stronger associations with the recognition and characteristics of the underlying brand asset, consistent with their heterogeneous properties. This paper was accepted by Suraj Srinivasan, accounting. Funding: I acknowledge the financial support from my advisor, Eddie Riedl, as well as from the UNH Paul College of Business and Economics Summer Research Support Award. Supplemental Material: The online appendix and data files are available at https://doi.org/10.1287/mnsc.2022.00387 .
HINDFOOT ANKLE RECONSTRUCTION NAIL TRIAL (HARNT): POPULATION ANALYSIS OF SURGICAL TREATMENT OF COMPLEX ANKLE FRACTURES IN THE UNITED KINGDOM
Authors: Ahluwalia R. , Coffey D. , Reichert I. , Stringfellow T. , Wek C. , Tan Sze Ping 
Published: May 2024

IntroductionThe management of open or unstable ankle and distal tibial fractures pose many challenges. In certain situations, hindfoot nailing (HFN) is indicated, however this depends on surgeon preference and regional variations exist. This study sought to establish the current management and outcomes of complex ankle fractures in the UK.MethodsA National collaborative study in affiliation with BOTA was conducted and data retrospectively collected between January 1st – June 30th 2019. Adult patients with open and closed complex ankle fractures (AO43/44) were included. Complex fractures included the following patient characteristics: diabetes ± neuropathy, rheumatoid arthritis, alcoholism, polytrauma and cognitive impairment. We obtained data on fixation choice and patient outcomes. Institutional approval was obtained by all centres, and statistical analysis was performed including propensity matching.ResultsFifty-six centres provided data for 1360 patients. The average age of the cohort was 53.9 years ±19 (SD) with a male/female ratio of 1:1.3. 920 patients were ASA 1/2, 440 were ASA 3/4; 316 had diabetes, and 275 were open fractures. Most fractures were AO44 (71.2%) and more commonly associated with diabetes (P<0.001), alcoholism (P<0.007), open (P<0.013), and advanced age (55.7 vs. 46.3). 1227 patients underwent primary-fixation (111 HFN), with the remainder treated with external-fixation (84 definitive). Of the 111 HFN, 35% underwent primary fusion. Wound complication and thromboembolic rates were greater in the HFN group compared to ORIF groups (P<0.003), being more evident in the HFN group with primary fusion even after propensity matching. However, 1081 patients were non-weightbearing post-op contrary to the BOAST guidance.ConclusionThis is the first National collaborative audit of complex ankle fractures. Hindfoot nails were used in 9% of patients and we observed more complications in this group when compared to other cohorts. Despite BOAST guidance, only 21% of patients undergoing operative management were instructed to fully weightbear post-operatively.
The Many Meanings of Mina: Popular Music Stardom in Post-war Italy, Rachel Haworth (2022)
Authors: Mosconi Elena 
Published: May 2024

Review of: The Many Meanings of Mina: Popular Music Stardom in Post-war Italy, Rachel Haworth (2022)
Bristol and Chicago, IL: Intellect, 272 pp.,
ISBN 978-1-78938-560-1, h/bk, GBP 85.00
Pasta, Pizza and Propaganda: A Political History of Italian Food TV, Francesco Buscemi (2022)
Authors: Tominc Ana 
Published: May 2024

Review of: Pasta, Pizza and Propaganda: A Political History of Italian Food TV, Francesco Buscemi (2022)
Bristol: Intellect, 170 pp.,
ISBN 978-1-78938-406-2, h/bk, £85.00
Il libro bianco di Pasolini: La raccolta dei processi a Pier Paolo Pasolini, Francesco Aliberti, Alessandro Di Nuzzo and Enzo Lavagnini (2022)
Authors: Tonelli Anna 
Published: May 2024

Review of: Il libro bianco di Pasolini: La raccolta dei processi a Pier Paolo Pasolini, Francesco Aliberti, Alessandro Di Nuzzo and Enzo Lavagnini (2022)
Reggio Emilia: Compagnia editoriale Aliberti, 378 pp.,
ISBN 978-8-89323-464-1, p/bk, €19.00
Thermodynamic and kinetic study on the catalysis of tributyl aconitate by Amberlyst-15 in a cyclic fixed-bed reactor
Authors: Deng Ailin , Ma Qiqi , Hu Yunxiang , Zhang Xin , Yang Sangni , Song Nianhua , Sun Wuji , Liu Xuejun , Ji Jianbing 
Published: May 2024

Abstract
Tributyl aconitate is a new type of alternative plasticizer to phthalates. Amberlyst-15 was used to catalyze the esterification of aconitic acid and n-butanol for the preparation of tributyl aconitate in a cyclic fixed-bed reactor. The influence of the reaction conditions on the conversion was investigated. The results showed that the conversion of aconitic acid increased significantly with the rise of temperature and catalyst loading. The reaction conditions were optimized as: temperature: 115 °C; initial mass ratio of AA and n-butanol: 1:4; catalyst loading: 25 %; reaction absolute pressure: 85 kPa; volume flow rate: 30 mL min−1. Thermodynamics and kinetics of the reaction was studied. The non-ideality of the reaction system was rectified using the UNIFAC group contribution method. The kinetic process was simulated using the pseudo-homogeneous (PH) model, Eley-Rideal (E-R) model, and Langmuir-Hinshelwood-Hougen-Watson (LHHW) model. The results revealed that the E-R model exhibited superior suitability in simulating the kinetic process.
Development and characterization of a coaxial strain-sensing cable integrated steel strand for wide-range stress monitoring
Authors: Jiao Tong , Pu Chuhong , Xu Qiang , Tang Minggao , Zhu Xing , Liu Chuankun , Li Jiang 
Published: May 2024

Abstract
Monitoring the stress of steel strands, from initial tension to eventual failure, is paramount for assessing structural safety and understanding its failure mechanism. Current monitoring methods are restricted in measuring stress only until yielding because of their limited range. This study proposes a novel coaxial strain-sensing cable (CSSC) based intelligent steel strand (CSSC-ISS), which has both functions of force-bearing and self-sensing. First, the prototype design of CSSC-ISS and the sensing principle of CSSC are introduced. Then, a fabrication method of small-diameter CSSC is proposed, which is then encapsulated with glass fiber reinforced polymer (GFRP) material, forming a GFRP sensing rod (GFRP-SR). The next step involves replacing the strand’s central wire with the GFRP-SR, culminating in the creation of the CSSC-ISS. Finally, Laboratory tests show that the CSSC has excellent strain-sensing performance with a resolution of at least 100 µε and a measurement range of 150,000 µε. The GFRP-SR offers good sensing potential and comparable mechanical strength to standard GFRP rods. Notably, the CSSC-ISS could measure stress up to strand failure, retaining 87.9% tensile strength and 88.7% elastic modulus compared to standard steel strands. It is verified that the CSSC-ISS can consistently measure its stress condition throughout its life cycle without compromising its load-bearing potential.
The Lost “Linant de Bellefonds Stela” of the Chief-Treasurer Maya and its Hymn and Prayer to the Sun-God
Authors: Pasquali Stéphane 
Published: May 2024

Summary
Publication of a new stela of the Chief-Treasurer Maya (time of Tutankhamun-Horemheb) with a long hymn addressed to the rising sun. The closest versions of the text are those of pNakht (London BM EA 10471, 21), the London stela of Horemheb (BM EA 551), and the pyramidion of Amenhotep Huy in the Rijksmuseum van Oudheden, Leiden (AM 6-b) – one sequence about the sun rays and the Haunebut (line 5) is original in view of the available documentation.
The stela (now lost) which was in the possession of Linant de Bellefonds in 1850, is only known thanks to a handwritten copy by Auguste Mariette. There can be no doubt that it comes from the tomb of Maya at Saqqara.
Prediction of Poor Outcome Using the Urea to Albumin Ratio in Thoracic Empyema
Authors: Dimitrov Evgeni , Valchev Daniel , Minkov Georgi , Enchev Emil , Yovtchev Yovcho 
Published: May 2024

Purpose: The prognostic performance of urea-to-albumin ratio (UAR) has been assessed in various pulmonary and nonpulmonary conditions, but never in thoracic empyema. Therefore, our aim was to determine whether this marker has the ability to predict outcome in such patients.Methods: A single-center retrospective study was conducted in a Clinic of Thoracic Surgery at a University Hospital between January 2021 and October 2023. A total of 84 patients who underwent emergency surgery due to thoracic empyema were involved. Serum levels of urea and albumin at admission were used to calculate UAR. We analyzed area under receiver operating characteristics (AUROC) curves of UAR, systemic inflammatory response syndrome (SIRS) and quick-sequential organ failure assessment (qSOFA), and compared their prognostic performance.Results: The identified in-hospital mortality was 10.7%. The UAR showed the best ability to prognosticate mortality compared to qSOFA (AUROC = 0.828 vs 0.747) and SIRS (AUROC = 0.828 vs 0.676). We established a sensitivity of 87.5% and specificity of 74.2% at optimal cut-off value UAR > 51.1 for prediction of adverse outcome.Conclusion: In patients with thoracic empyema urea-to-albumin ratio showed significant prognostic performance and a potential for clinical application as a low cost and widely available predictor of death.
Aronia melanocarpa fruit juice prevents hepatic impairment in rats subjected to metabolic syndrome
Authors: Reyzov M. , Eftimov M. , Gancheva S. , Todorova M. , Zhelyazkova-Savova M. , Tzaneva M. , Valcheva-Kuzmanova S. 
Published: May 2024

AbstractThis study evaluated the effects of Aronia melanocarpa fruit juice (AMFJ) on the liver in a model of high-fat, high-fructose (HFHF) diet-induced metabolic syndrome (MS). Fifty rats were allocated to five groups – Control, MS, MS+AMFJ2.5, MS+AMFJ5, and MS+AMFJ10. The control group was fed a standard diet, while the other groups were provided a HFHF diet. During MS induction, daily oral treatment was performed. Control and MS groups received 10.0 mL kg−1 distilled water, while the other three groups received AMFJ at doses of 2.5, 5.0, and 10.0 mL kg−1, respectively. After 10 weeks, liver samples were collected and inspected histologically as well as immunohistochemically to determine the expression of Bax, Bcl-2, and MAC387. Bax/Bcl-2 ratio was calculated. In MS rats, steatotic, inflammatory and degenerative alterations of the liver were detected, Bax and MAC387 were markedly elevated, while Bcl-2 was non-significantly reduced. The Bax/Bcl-2 ratio significantly increased. The histopathological alterations were prevented by the AMFJ treatment. Compared to MS group, Bax and MAC387 values were significantly lower and Bcl-2 value was higher resulting in significantly lower (P < 0.001) Bax/Bcl-2 ratio in all AMFJ-treated groups. AMFJ, administered during MS induction in rats, prevented the occurrence of inflammatory, steatotic, degenerative, and pro-apoptotic changes in the liver.
Determination of fraxetin, fraxin and dimethylfraxetin in rat plasma by UPLC-MS/MS
Authors: Jia Qing , Wang Ziyue , Wang Shuqian , Ma Shunjun , Qiao Xueqi , Huang Xueli , Wang Xianqin , Wen Congcong , Zhu Xia-yin 
Published: May 2024

AbstractObjectiveThe levels of fraxetin, fraxin, and dimethylfraxetin in rat plasma to be measured using an ultra-performance liquid chromatography tandem mass-spectrometry (UPLC–MS/MS) technique and applied to their pharmacokinetics and bioavailability.MethodsThe protein precipitation technique was applied to the plasma preparation using acetonitrile and methanol (9:1, v/v). At a flow rate of 0.4 mL min−1, the elution time was 6 min. The mobile phase consisted of acetonitrile-water with 0.1% formic acid, and the chromatographic column was UPLC HSS T3 (50 mm × 2.1 mm, 1.8 μm). Quantitative analysis was conducted using multiple reaction monitoring (MRM) mode and detection was performed using electrospray ionization (ESI) positive ion mode. In each group, six rats were treated with fraxetin, fraxin, and dimethylfraxetin either orally (5 mg kg−1) or intravenously (1 mg kg−1).ResultsThe calibration curves showed good linearity in the range of 2–4,000 ng mL−1, where r was greater than 0.99. The bioavailability of dimethylfraxetin, fraxin, and fraxetin was determinated to be 19.7, 1.4, and 6.0%.ConclusionThe established UPLC-MS/MS method for determining the levels of these three compounds in rat plasma was successfully applied to the pharmacokinetics of dimethylfraxetin, fraxin, and fraxetin, and the bioavailability was calculated.