bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of nanosilica content on properties of polyurethane/silica hybrid emulsion and its films
Authors: Wang Lei , Shen Yiding , Lai Xiaojuan , Li Zhongjin 
Published: September 2010

AbstractPolyurethane/silica hybrid emulsion (PUSi) was synthesized by the reaction of isophorone isocyanate, polyether polyol, hydrophilic nanosilica (A200), dimethylol propionic acid, trimethylol propane, and 3‐aminopropyltriethoxysilane (KH550). The films of the waterborne polyurethane (WPU) were prepared. The structure of the polyurethane was characterized by Fourier transform infrared spectrometer (FTIR), thermogravimetry (TG), and differential scanning calorimetry (DSC). The particle size distribution and morphology of emulsion were examined. Influence of nanosilica content on the mechanical properties and solvent absorption of the cast films were also measured quantificationally. FTIR indicates that NH2 of KH550 reacted with NCO of polyurethane. TG analysis indicates that nanosilica can improve thermal stability of polyurethane. There is no clear effect of nanosilica on the glass transition of soft segments. It was found that greater mechanical properties of WPU were obtained when chemical networks were formed by sol‐gel process. As the nanosilica content increases, water absorption and ethanol absorption decreased. The particle size increases with increase of A200 content. PUSi hybrid emulsions are endowed with pseudoplasticity. The apparent viscosity of emulsions increased and then decreased with addition of A200. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Sulphur‐vulcanized polybutadiene as a matrix in glass fiber‐reinforced composite materials
Authors: Prokůpek Luboš , Večeřa Miroslav , Machotová Jana , Šňupárek Jaromír 
Published: September 2010

AbstractThe basic material used in this work was a low‐molecular‐weight polybutadiene with isocyanate endgroups in the main chain. The isocyanate groups were used for crosslinking of the oligomeric polybutadiene with glycerol as a three‐functional crosslinker. The prepared polybutadiene‐based polyurethane gel was subsequently vulcanized with sulphur. The effect of sulphur content on mechanical and electrical properties of resulting materials was investigated with the aim to find an optimum matrix composition for the preparation of composite materials. Several types of glass fiber fabric reinforcement differing in fabric weight and fabric ply thickness were tested. Mechanical properties of composites based on the optimum matrix composition and different types of glass fibers were measured and compared. Being vulcanized with sulphur, the polybutadiene was found to possess improved mechanical properties and retain an excellent electroinsulating character. Moreover, the sulphur‐vulcanized polybutadiene was proved good as a matrix for the preparation of glass fiber‐reinforced composite materials having enhanced tensile and flexural properties. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Monoclonal anti‐CD8 therapy induces disease amelioration in the K/BxN mouse model of spontaneous chronic polyarthritis
Authors: Raposo Bruno R. , Rodrigues‐Santos Paulo , Carvalheiro Helena , Água‐Doce Ana M. , Carvalho Lina , Pereira da Silva José A. , Graça Luis , Souto‐Carneiro M. Margarida 
Published: September 2010

AbstractObjectiveCD8+ T cells are part of the T cell pool infiltrating the synovium in rheumatoid arthritis (RA). However, their role in the pathogenesis of RA has not been fully delineated. Using the K/BxN mouse model of spontaneous chronic arthritis, which shares many similarities with RA, we studied the potential of CD8+ T cell depletion with monoclonal antibodies (mAb) to stop and reverse the progression of experimental arthritis.MethodsCD8+ T cells from the blood and articular infiltrate of K/BxN mice were characterized for cell surface phenotypic markers and for cytokine production. Additionally, mice were treated with specific anti‐CD8 mAb (YTS105 and YTS169.4), with and without thymectomy.ResultsCD8+ T cells from the peripheral blood and joints of K/BxN mice were mainly CD69+ and CD62L−CD27+ T cells expressing proinflammatory cytokines (interferon‐γ [IFNγ], tumor necrosis factor α [TNFα], interleukin‐17a [IL‐17A], and IL‐4), and granzyme B. In mice receiving anti‐CD8 mAb, the arthritis score improved 5 days after treatment. Recovery of the CD8+ T cells was associated with a new increase in the arthritis score after 20 days. In thymectomized and anti‐CD8 mAb–treated mice, the arthritis score improved permanently. Histologic analysis showed an absence of inflammatory infiltrate in the anti‐CD8 mAb–treated mice. In anti‐CD8 mAb–treated mice, the serologic levels of TNFα, IFNγ, IL‐6, and IL‐5 normalized. The levels of the disease‐related anti–glucose‐6‐phosphate isomerase antibodies did not change.ConclusionThese results indicate that synovial activated effector CD8+ T cells locally synthesize proinflammatory cytokines (IFNγ, TNFα, IL‐17, IL‐6) and granzyme B in the arthritic joint, thus playing a pivotal role in maintaining chronic synovitis in the K/BxN mouse model of arthritis.
Efficacy and safety of an intraoral electrostimulation device for xerostomia relief: A multicenter, randomized trial
Authors: Strietzel Frank P. , Lafaurie Gloria I. , Mendoza Gloria R. Bautista , Alajbeg Ivan , Pejda Slavica , Vuletić Lea , Mantilla Rubén , Falcão Denise P. , Leal Soraya C. , Bezerra Ana C. Barreto , Tran Simon D. , Ménard Henri A. , Kimoto Suguru , Pan Shaoxia , Martín‐Granizo Rafael A. , Lozano M. Lourdes Maniegas , Zunt Susan L. , Krushinski Cheryl A. , Melilli Dario , Campisi Giuseppina , Paderni Carlo , Dolce Sonia , Yepes Juan F. , Lindh Liselott , Koray Meltem , Mumcu Gonca , Elad Sharon , Zeevi Itai , Barrios Beatriz C. Aldape , López Sánchez Rodrigo M. , Beiski Ben Z. , Wolff Andy , Konttinen Yrjö T. 
Published: September 2010

AbstractObjectiveTo evaluate the efficacy and safety of an intraoral electrostimulation device, consisting of stimulating electrodes, an electronic circuit, and a power source, in treating xerostomia. The device delivers electrostimulation through the oral mucosa to the lingual nerve in order to enhance the salivary reflex.MethodsThe device was tested on a sample of patients with xerostomia due to Sjögren's syndrome and other sicca conditions in a 2‐stage prospective, randomized, multicenter trial. Stage I was a double‐blind, crossover stage designed to compare the effects of the electrically active device with the sham device, each used for 1 month, and stage II was a 3‐month open‐label stage designed to assess the long‐term effects of the active device. Improvement in xerostomia severity from baseline was the primary outcome measure.ResultsA total of 114 patients were randomized. In stage I, the active device performed better than the sham device for patient‐reported xerostomia severity (P < 0.002), xerostomia frequency (P < 0.05), quality of life impairment (P < 0.01), and swallowing difficulty (P < 0.02). At the end of stage II, statistically significant improvements were verified for patient‐reported xerostomia severity (P < 0.0001), xerostomia frequency (P < 0.0001), oral discomfort (P < 0.001), speech difficulty (P < 0.02), sleeping difficulty (P < 0.001), and resting salivary flow rate (P < 0.01).ConclusionOur findings indicate that daily use of the device alleviated oral dryness, discomfort, and some complications of xerostomia, such as speech and sleeping difficulties, and increased salivary output. The results show a cumulative positive effect of the device over the period of the study, from baseline to the end of the trial.
V‐region gene analysis of locally defined synovial B and plasma cells reveals selected B cell expansion and accumulation of plasma cell clones in rheumatoid arthritis
Authors: Scheel Tobias , Gursche Angelika , Zacher Josef , Häupl Thomas , Berek Claudia 
Published: September 2010

AbstractObjectiveTo elucidate the development of synovial tissue–specific B cell immune responses, the clonality of individual naive B cells, memory B cells, and plasma cells and their organization and histologic localization in the inflamed tissue were investigated in patients with rheumatoid arthritis (RA).MethodsB and plasma cells were isolated by laser capture microdissection (LCM) from the synovial tissue of patients with RA. In addition, single naive B cells, memory B cells, and plasma cells were sorted from synovial tissue cell suspensions. RNA was extracted from the cells, and Ig VH genes were amplified, cloned, and sequenced.ResultsBoth LCM and single cell sorting analyses showed that naive and memory B cells infiltrated the RA synovial tissue. Comparison of the V‐gene repertoire of B and plasma cells suggested that synovial plasma cells were generated, by and large, from locally activated B cells, indicating that a selected population of memory B cells differentiates into large plasma cell clones that then accumulate in the inflamed tissue. Clonally related plasma cells were isolated from separate and distinct localized areas of the tissue, suggesting that the newly generated plasma cells have a high migratory capacity.ConclusionThese results support the idea of a continuous activation of selected B cell clones, and hence a massive accumulation of plasma cells, in RA synovial tissue. As B cells and their secreted antibodies are an important factor in controlling inflammatory processes, patients with RA displaying intensive synovial tissue lymphocytic infiltrations might benefit from B cell depletion therapy. Early treatment will prevent accumulation of pathogenic plasma cells.
Protein–protein interactions in transcription: A fertile ground for helix mimetics
Authors: Guarracino Danielle A. , Bullock Brooke N. , Arora Paramjit S. 
Published: September 2010

AbstractDesigned ligands that inhibit protein–protein interactions involved in gene expression are valuable as reagents for genomics research and as leads for drug discovery efforts. Selective modulation of protein–protein interactions has proven to be a daunting task for synthetic ligands; however, the last decade has seen significant advances in inhibitor design, especially for helical protein interfaces. This review discusses examples of transcriptional complexes targeted by designer helices. © 2010 Wiley Periodicals, Inc. Biopolymers 95: 1–7, 2011.
Production of heparanase constructs suitable for nuclear magnetic resonance and drug discovery studies
Authors: Mosulén Silvia , Ortí Leticia , Bas Esperanza , Carbajo Rodrigo J. , Pineda‐Lucena Antonio 
Published: September 2010

AbstractHeparanase is an endo‐β‐D‐glucosidase capable of specifically degrading heparan sulphate, one of the main components of the extracellular matrix. This 65 kDa polypeptide is implicated in cancer processes such as tumour formation, angiogenesis and metastasis, making it a very attractive target in antitumour treatments. Structure‐based approaches to find inhibitors of heparanase have been historically hampered by the lack of success in crystallizing the protein. With the aim to undertake the NMR structural characterisation of heparanase, we have designed and produced, using recombinant methods, smaller constructs of heparanase containing the catalytically active glutamic acids and the two binding sites for heparan sulphate. An extensive range of expression and purification conditions were evaluated to alleviate the intrinsic low solubility and aggregation propensity of heparanase, allowing the obtention of the enzyme in milligram quantities, both unlabelled and 15N‐labelled for NMR studies. Using the smallest of the designed constructs and applying NMR and SPR methodologies, we have demonstrated that known inhibitors of heparanase bind to this construct specifically and selectively with KD values in the range of those reported for human heparanase, validating it for future drug discovery projects focused on the identification of novel inhibitors of this enzyme. © 2010 Wiley Periodicals, Inc. Biopolymers 95: 151–160, 2011.
Inhibition of hypoxia‐inducible transcription factor complex with designed epipolythiodiketopiperazine
Authors: Kushal Swati , Wang Hui , László Csaba F. , Szábo Lajos Z. , Olenyuk Bogdan Z. 
Published: September 2010

AbstractDesigned small molecule inhibitors of hypoxia‐inducible gene expression have potential to become new research tools for molecular biology, genetics and serve as leads to new therapeutics. We report design, synthesis evaluation of biological activity, and a preliminary mechanistic study of epipolythiodiketopiperazine (ETP) transcriptional antagonist that targets the interaction between the C‐terminal transactivation domain (C‐TAD) of hypoxia‐inducible factor 1α (HIF‐1α) and cysteine‐histidine rich region (CH1) of transcriptional coactivator p300/CBP. Our results indicate that in cultured cells synthetic ETP 3 disrupts the structure and function of this complex in a dose‐dependent manner, resulting in rapid downregulation of hypoxia‐inducible gene expression. © 2010 Wiley Periodicals, Inc. Biopolymers 95: 8–16, 2011.
Dynamic flux balance modeling of microbial co‐cultures for efficient batch fermentation of glucose and xylose mixtures
Authors: Hanly Timothy J. , Henson Michael A. 
Published: September 2010

AbstractSequential uptake of pentose and hexose sugars that compose lignocellulosic biomass limits the ability of pure microbial cultures to efficiently produce value‐added bioproducts. In this work, we used dynamic flux balance modeling to examine the capability of mixed cultures of substrate‐selective microbes to improve the utilization of glucose/xylose mixtures and to convert these mixed substrates into products. Co‐culture simulations of Escherichia coli strains ALS1008 and ZSC113, engineered for glucose and xylose only uptake respectively, indicated that improvements in batch substrate consumption observed in previous experimental studies resulted primarily from an increase in ZSC113 xylose uptake relative to wild‐type E. coli. The E. coli strain ZSC113 engineered for the elimination of glucose uptake was computationally co‐cultured with wild‐type Saccharomyces cerevisiae, which can only metabolize glucose, to determine if the co‐culture was capable of enhanced ethanol production compared to pure cultures of wild‐type E. coli and the S. cerevisiae strain RWB218 engineered for combined glucose and xylose uptake. Under the simplifying assumption that both microbes grow optimally under common environmental conditions, optimization of the strain inoculum and the aerobic to anaerobic switching time produced an almost twofold increase in ethanol productivity over the pure cultures. To examine the effect of reduced strain growth rates at non‐optimal pH and temperature values, a break even analysis was performed to determine possible reductions in individual strain substrate uptake rates that resulted in the same predicted ethanol productivity as the best pure culture. Biotechnol. Bioeng. 2011;108: 376–385. © 2010 Wiley Periodicals, Inc.
MATLAB‐SIMULINK‐based simulation for digital differential relay protection of power transformer for educational purpose
Authors: Vahidi B. , Esmaeeli E. 
Published: September 2010

AbstractDifferential protection was already applied toward the end of the 19th century and was one of the first protection systems ever used. Faults are detected by comparison of the currents flowing into and out of the protected plant item. As a result of the fast tripping with absolute selectivity, it is suited as main protection of all important items of plant. The article presents an approach to teaching relay laboratory using specially designed exercises that can be done using MATLAB‐SIMULINK. This article presents a MATLAB‐SIMULINK‐based technology to simulate differential relay for determining behavior of it during transformer internal fault protection. The results show that this simulation method can work properly and this design can be used for power system simulation in MATLAB‐SIMULINK. Evaluation of the simulation with more than 60 students is very positive in terms of their developing confidence in and understanding of this kind of relay operation. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 21: 475–483, 2013
Adaptive interactions in shared virtual environments for heterogeneous devices
Authors: Kim Soo Jeong , Gil Kyung Jun , Kim HyungSeok , Lim Sang Boem , Kim Jee‐in 
Published: September 2010

AbstractIn this research, we propose a method to adapt the interfaces and interaction processes of heterogeneous devices. The proposed method models the interaction capability of the devices. The interaction is modeled by categorizing elementary actions and measuring its effect in semantic behaviors. With this model, an interaction process can be modified to a given device by changing sequence of elementary actions for each behavior. In the pilot test, we showed the possibility of interaction adaptation for different situations. With the proposed adaptation mechanism, interface and interaction can be modeled for the device independently and can be transferred over different interaction environments. Copyright © 2010 John Wiley & Sons, Ltd.
Structural influence of chiral tertiary aminonaphthol ligands on the asymmetric phenyl transfer to aromatic aldehydes
Authors: Wei Hui , Yin Lu , Luo Haibin , Li Xingshu , Chan Albert S. C. 
Published: September 2010

AbstractA series of chiral tertiary aminonaphthol ligands were prepared from 2‐naphthol, (S)‐1‐phenylethylamine, and aldehydes with diverse substituted groups. The results of asymmetric phenyl transfer to aromatic aldehydes catalyzed by these chiral ligands indicated that enantioselectivities were greatly influenced by the electronic and steric effects of the ligands. Chirality, 2011. © 2010 Wiley‐Liss, Inc.
The comparison in enantioseparation ability of the chiral stationary phases with single and mixed selector—The selectors derived from two D‐tartrates
Authors: Chen Jun , Li Mu‐Zi , Xiao Yan‐Hua , Chen Wei , Li Shi‐Rong , Bai Zheng‐Wu 
Published: September 2010

Abstract(2S,3S)‐2,3‐Bis(3,5‐dimethylphenylcarbonyloxy)‐3‐(benzyloxycarbonyl)‐propanoic acid and (2S,3S)‐2,3‐bis(1‐naphthalenecarbonyloxy)‐3‐(benzyloxycarbonyl)‐propanoic acid were synthesized from D‐tartaric acid. These two compounds were chlorinated to afford two chiral selectors for chiral stationary phases (CSPs). The selectors were separately immobilized on aminated silica gel to give two single selector CSPs; and were simultaneously immobilized to obtain a mixed selector CSP. Comparing to the single selector CSPs, the mixed selector CSP bears the enhanced enantioseparation ability, suggesting that the two selectors in the mixed selector CSP are consistent for chiral recognition in most mobile phase conditions. Chirality, 2011. © 2010 Wiley‐Liss, Inc.
Effectiveness of population‐based service screening with mammography for women ages 40 to 49 years
Authors: Hellquist Barbro Numan , Duffy Stephen W. , Abdsaleh Shahin , Björneld Lena , Bordás Pál , Tabár László , Viták Bedrich , Zackrisson Sophia , Nyström Lennarth , Jonsson Håkan 
Published: September 2010

AbstractBACKGROUND:The effectiveness of mammography screening for women ages 40 to 49 years still is questioned, and few studies of the effectiveness of service screening for this age group have been conducted.METHODS:Breast cancer mortality was compared between women who were invited to service screening at ages 40 to 49 years (study group) and women in the same age group who were not invited during 1986 to 2005 (control group). Together, these women comprise the Mammography Screening of Young Women (SCRY) cohort, which includes all Swedish counties. A prescreening period was defined to facilitate a comparison of mortality in the absence of screening. The outcome measure was refined mortality, ie, breast cancer death for women who were diagnosed during follow‐up at ages 40 to 49 years. Relative risks (RRs) with 95% confidence intervals (CIs) were estimated.RESULTS:There was no significant difference in breast cancer mortality during the prescreening period. During the study period, there were 803 breast cancer deaths in the study group (7.3 million person‐years) and 1238 breast cancer deaths in the control group (8.8 million person‐years). The average follow‐up was 16 years. The estimated RR for women who were invited to screening was 0.74 (95% CI, 0.66‐0.83), and the RR for women who attended screening was 0.71 (95% CI, 0.62‐0.80).CONCLUSIONS:In this comprehensive study, mammography screening for women ages 40 to 49 years was efficient for reducing breast cancer mortality. Cancer 2011. © 2010 American Cancer Society.
Generating web‐based user interfaces for computational science
Authors: van Hemert Jano , Koetsier Jos , Torterolo Livia , Porro Ivan , Melato Maurizio , Barbera Roberto 
Published: September 2010

AbstractScientific gateways in the form of web portals are becoming the popular approach to share knowledge and resources around a topic in a community of researchers. Unfortunately, the development of web portals is expensive and requires specialists skills. Commercial and more generic web portals have a much larger user base and can afford this kind of development. Here we present two solutions that address this problem in the area of portals for scientific computing; both take the same approach. The whole process of designing, delivering and maintaining a portal can be made more cost‐effective by generating a portal from a description rather than programming in the traditional sense. We show four successful use cases to show how this process works and the results it can deliver. Copyright © 2010 John Wiley & Sons, Ltd.
From freud to a modern understanding of behavioral, physiological, and brain development
Authors: Stern Judith M. , Weinberg Joanne , Hennessy Michael B. 
Published: September 2010

AbstractSeymour Levine's first “early experience” experiments were inspired by Freud. Yet, Levine's lifetime of work, and the work of his colleagues and scientists who followed, unveiled a myriad of early experience effects that even Freud himself could not have imagined. Related to and extending beyond his work on early experience, Levine also made important, often seminal, contributions to overlapping and related areas, such as early maternal separation and deprivation, maternal behavior and physiology, sexual differentiation, perinatal malnutrition, attachment in non‐human primates, hypothalamic‐pituitary‐adrenal (HPA) stress reactivity and its adaptive significance, and the development of the HPA system. Moreover, his work spawned new lines of research by investigators active today. The papers contained in this special issue provide a sampling of research demonstrating some of the important directions in which those earliest experiments have led, many with clinical applications. © 2010 Wiley Periodicals, Inc. Dev Psychobiol 52: 609–615, 2010.