bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anthocyanins from red cabbage extract — evidence of protective effects on blood platelets
Authors: Saluk Joanna , Bijak Michał , Kołodziejczyk-Czepas Joanna , Posmyk Małgorzata , Janas Krystyna , Wachowicz Barbara 
Published: June 2012

AbstractRed cabbage belongs to cruciferous vegetables recognized as a rich source of anthocyanins. Anthocyanins have a wide range of therapeutic advantages without adverse effects, including cardiovascular protective properties. For development of cardiovascular diseases, platelet activation is crucial; therefore compounds which inhibit platelet activation are sought after. The anti-platelet activity of anthocyanins has only been described and is still unclear. In our study, the extract of anthocyanins, obtained from fresh leaves of red cabbage, was used in vitro to examine their antioxidative effects on platelets under oxidative stress conditions which are responsible for hyperactivity of these cells. The antiplatelet and antioxidative activities were determined by platelet aggregation and specific markers of the arachidonate cascade with O2−· generation, and oxidative changes (carbonyl groups and 3-nitrotyrosine). Extracts (5–15 μM) protected platelet proteins and lipids against oxidative damage, and diminished platelet activation. Anthocyanins from red cabbage provided beneficial anti-platelet effects and might help prevent cardiovascular diseases.
Rhizobial plasmids — replication, structure and biological role
Authors: Mazur Andrzej , Koper Piotr 
Published: June 2012

AbstractSoil bacteria, collectively named rhizobia, can establish mutualistic relationships with legume plants. Rhizobia often have multipartite genome architecture with a chromosome and several extrachromosomal replicons making these bacteria a perfect candidate for plasmid biology studies. Rhizobial plasmids are maintained in the cells using a tightly controlled and uniquely organized replication system. Completion of several rhizobial genome-sequencing projects has changed the view that their genomes are simply composed of the chromosome and cryptic plasmids. The genetic content of plasmids and the presence of some important (or even essential) genes contribute to the capability of environmental adaptation and competitiveness with other bacteria. On the other hand, their mosaic structure results in the plasticity of the genome and demonstrates a complex evolutionary history of plasmids. In this review, a genomic perspective was employed for discussion of several aspects regarding rhizobial plasmids comprising structure, replication, genetic content, and biological role. A special emphasis was placed on current post-genomic knowledge concerning plasmids, which has enriched the view of the entire bacterial genome organization by the discovery of plasmids with a potential chromosome-like role.
In vitro propagation of Gentiana dinarica Beck.
Authors: Vinterhalter Branka , Milošević Dijana , Janković Teodora , Milojević Jelena , Vinterhalter Dragan 
Published: June 2012

AbstractGentiana dinarica Beck, rare and endangered species of Balkan Dinaric alps, was in vitro propagated (micropropagated) from axillary buds of plants collected at Mt. Tara, Serbia. G. dinarica preferred MS to WPM medium, with optimal shoot multiplication on MS medium with 3% sucrose, 1.0 mg l−1 BA and 0.1 mg l−1 NAA. Rooting was not clearly separated from shoot multiplication since BA did not completely inhibit root initiation. Spontaneous rooting on plant growth regulator-free medium occurred in some 30% of shoot explants. Rooting was stimulated mostly by decreased mineral salt nutrition and a medium with 0.5 MS salts, 2% sucrose and 0.5–1.0 mg l−1 IBA was considered to be optimal for rooting. Rooted plantlets were successfully acclimated and further cultured in peat-based substrate.
Natural rules for Arabidopsis thaliana pre-mRNA splicing site selection
Authors: Wu Ning , Matand Kanyand , Wu Huijuan , Li Baoming , Love Kayla , Stoutermire Brittany , Wu Yanfeng 
Published: June 2012

AbstractThe accurate prediction of plant pre-mRNA splicing sites has been studied extensively. The rules for plant pre-mRNA splicing still remain unknown. This study, based on confirmed sequence data, systematically analyzed all expressed genes on Arabidopsis thaliana chromosome IV to quantitatively explore the natural splicing rules. The results indicated that defining Arabidopsis thaliana pre-mRNA splicing sites required a combination of multiple factors including (1) relative conserved consensus sequence at splicing site; (2) individual nucleotide distribution pattern in 50 nucleotides up- and down-stream regions of splicing site; (3) quantitative analysis of individual nucleotide distribution by using the formulations concluded from this study. The combination of all these factors together can bring the accuracy of Arabidopsis thaliana splicing site recognition over 99%. The results provide additional information to the future of plant pre-mRNA splicing research.
Screening of rootstock hybrids with Vitis cinerea Arnold for phylloxera resistance
Authors: Pavloušek Pavel 
Published: June 2012

AbstractThe resistance to phylloxera is one of the most important properties of grapevine rootstock. The breeding and selection of rootstock varieties represents a biological method of fight against phylloxera. In this study altogether 59 rootstock hybrids originating from nine different populations were evaluated. Pedigrees of all populations involved Vitis cinerea Arnold introduced through the hybrid Bruci/(Vitis berlandieri Planch. × Vitis rupestris Scheele) × Vitis cinerea Arnold/ and the variety Börner. Pot experiments were performed in a glasshouse while those with excised root pieces in root bioassay took place in a laboratory. This combination showed to be a very good selection tool for rootstock breeding. In seven rootstock hybrids, it was found that the resistance to phylloxera gallicoles and radicicoles was very high. The highest number of resistant hybrids was found in Binova × Börner. The variety Börner showed to be the best gene source of phylloxera resistance and even in case of a simple crossing produced sufficient number of resistant progeny.
Bioactive content and antioxidant capacity of Cape gooseberry fruit
Authors: Rop Otakar , Mlcek Jiri , Jurikova Tunde , Valsikova Magdalena 
Published: June 2012

AbstractAt present, Cape gooseberry (Physalis peruviana) fruit is one of the less used raw materials of plant origin, which can be used for human nutrition. This fruit, as well as alimentary products made of it, were used by healers in folk medicine in the distant past. The aim of this study was to monitor and evaluate the antioxidant capacity of fresh fruit of three Cape gooseberry cultivars ‘Giant’, ‘Golden berry’ and ‘Inka’. Antioxidant capacity was also tested, on the basis of the scavenging effect of reactive oxygen species (ROS) and lipid peroxidation of methanolic extracts made of fresh fruit. These results were further extended and supplemented with determinates of the vitamin C and total phenolic contents. These analyses were made for three consecutive years. The highest values of antioxidant capacity were observed in the ‘Inka’ cultivar (9.31 grams of ascorbic acid equivalents kg−1 of fresh mass). In this cultivar, the obtained results were corroborated also in ROS and the contents of vitamin C and total phenolics. Due to a high antioxidant capacity of this fruit species, the results presented should increase its popularity above all as a promising raw material, which can be used for human nutrition.
Plant tissue cultures from four Tuscan globe artichoke cultivars
Authors: Bedini Laura , Lucchesini Mariella , Bertozzi Francesco , Graifenberg Alberto 
Published: June 2012

AbstractThe aim of the study was to examine the possibility of propagating in vitro four of the most common cultivars in Tuscany (central Italy): Terom, Violetto di Toscana, Chiusure and Empolese. The first three belong to the “Violetti” group, while cv Empolese belongs to the “Romaneschi” group. Explants were cultured on an induction medium (IM), which is a modified MS medium consisting of nitrate concentrations reduced by one quarter, 0.8 mg L−1 6-benzylaminopurine (BA) and 0.2 mg L−1 3-indole butyric acid (IBA). Explants were then transferred to a proliferation medium (PM) consisting of the same basal medium together with 0.03 mg L−1 BA and 0.05 mg L−1 gibberellic acid (GA3). A rooting double-phase was then established. The pre-rooting medium (PRM), consisting of a basal MS medium with half strength nitrate concentrations, 0.5 mg L−1 indole-3-acetic acid (IAA) and 1 mg L−1 paclobutrazol (PBZ) was used for two weeks. Over the next four weeks, a rooting medium (MR) was used, consisting of a basal MS medium with 2 mg L−1 β-cyclodextrin and 2 mg L−1 α-naphthaleneacetic acid sodium salt (NAA). The cv Empolese provided the highest number of proliferated explants and rooted plantlets using the method described.
Quality Indicators of the Pre-Analytical Phase
Authors: Majkić-Singh Nada , Šumarac Zorica 
Published: June 2012

Quality Indicators of the Pre-Analytical PhaseQuality indicatorsare tools that allow the quantification of quality in each of the segments of health care in comparison with selected criteria. They can be defined as an objective measure used to assess the critical health care segments such as, for instance, patient safety, effectiveness, impartiality, timeliness, efficiency, etc. In laboratory medicine it is possible to develop quality indicators or the measure of feasibility for any stage of the total testing process. The total process or cycle of investigation has traditionally been separated into three phases, the pre-analytical, analytical and post-analytical phase. Some authors also include a »pre-pre« and a »post-post« analytical phase, in a manner that allows to separate them from the activities of sample collection and transportation (pre-analytical phase) and reporting (post-analytical phase). In the year 2008 the IFCC formed within its Education and Management Division (EMD) a task force calledLaboratory Errors and Patient Safety (WG-LEPS)with the aim of promoting the investigation of errors in laboratory data, collecting data and developing a strategy to improve patient safety. This task force came up with the Model of Quality Indicators (MQI) for the total testing process (TTP) including the pre-, intra- and post-analytical phases of work. The pre-analytical phase includes a set of procedures that are difficult to define because they take place at different locations and at different times. Errors that occur at this stage often become obvious later in the analytical and post-analytical phases. For these reasons the identification of quality indicators is necessary in order to avoid potential errors in all the steps of the pre-analytical phase.
Opportunities and restrictions for the local-endogenous development in metropolitan areas of high industrial concentration: the case of Thriasio Pedio in Attica
Authors: Christofakis Manolis , Tsampra Maria 
Published: June 2012

Opportunities and restrictions for the local-endogenous development in metropolitan areas of high industrial concentration: the case of Thriasio Pedio in AtticaThis paper investigates the development pattern of the urban area of Thriasio Pedio in the metropolitan region of Attica, which is characterised by a high concentration of industrial activities. The local-endogenous development model is discussed in the theoretical review of the paper, in the sense of the local socio-economic system's capacity to transform, react to external challenges, promote awareness and import specific forms of social regulation at the local level. On this ground, the main question of the paper concerns the nature of the area's development and more specifically, whether or not this is defined by endogenous factors (i.e. the operation of locally embedded production systems) along with predetermined exogenous factors (i.e. the allocation of central/metropolitan activities in Thriasio Pedio). The study is supported by the results of a sampling research in representative economic units of the Thriasio Pedio area. The analysis helped us to see whether the various applications of the local-endogenous development pattern, as defined in the paper, are incorporated into the overall productive system of the area. The prerequisites for the reinforcement of the local endogenous capacity were also identified in this analysis.
Possibilities of hyperlink application in spatial research
Authors: Janc Krzysztof 
Published: June 2012

Possibilities of hyperlink application in spatial researchThe main aim of the paper is to show the selected ways of analysing, the possible interpretations and expectations concerning the analyses of hyperlinks in spatial research. The connections existing in cyberspace for selected self-government websites of Lower Silesia were shown to illustrate the issue. The analyses were conducted for selected websites of self-government units at all levels functioning in Lower Silesia. The paper presents two approaches to the analysis of hyperlinks: analysis of outlinks and inlinks. The presented results allow us to identify some regularities regarding the functioning of connections in cyberspace versus the connections in the real world. From the perspective of self-government websites it can be concluded that the connections in cyberspace reflect the real connections. In the majority of the analysed cases there is a clear connection with the actual scope of activity. The remaining relationships with administrative cities reflect the significance of these cities for the functioning of self-government units in the social, economic, legal, and administrative conditions. Based on the conducted analyses it can be concluded that the study of hyperlinks may be useful in understanding the relationships between geographical space and cyberspace. They may form a new, interesting field of spatial research. We also found some challenges in the study of hyperlinks' spatial aspects: the identification of consistent criteria for determining the websites' ‘location in space’, the possibility of interpreting the research results and the dynamics of the Internet.
Characteristics of soaring birds' spring migration over inland SE Bulgaria
Authors: Shurulinkov Peter , Daskalova Girgina , Chakarov Nayden , Hristov Krassimir , Dyulgerova Silvia , Gocheva Yana , Cheshmedzhiev Svilen , Madzharov Minko , Dimchev Ivailo 
Published: June 2012

Characteristics of soaring birds' spring migration over inland SE Bulgaria
We investigated the scale and characteristics of spring migration of soaring birds in Bourgas district, SE Bulgaria during three 5-day periods in March (27th-31st), April (20th-24th) and May (12th-16th) 2008. Simultaneous counts were carried out from six observation points in two separate areas (Karnobat and Sredets), located 24.5 km and 37.3 km inland from the Black Sea coast, respectively. In total, 31,049 soaring birds of at least 27 species were counted, by far the most numerous being the White Stork Ciconia ciconia (23,358 ind.), followed by the Lesser Spotted Eagle Aquila pomarina (3,112 ind.). In Karnobat area, situated farther from the Black Sea coast, the total number of soaring migrants (21,231) was more than two-fold higher than in Sredets area (9,818). Overall, there was a significant difference in flock size and flight altitude within separate area (i.e. among observation points) as well as between the two observation areas and the three 5-day periods. However, there was a substantial variance in factors significantly affecting the flock size and flight altitude among most numerous individual species. In general, birds passed at much higher altitude above Karnobat area (mean flight altitude 208.3 m ± 6.4) compared to Sredets area (mean flight altitude 130.5 m ± 4.4). In both observation areas, northern direction was the most commonly observed flight direction (46.3% of all recorded flight directions). The highest numbers of soaring migrants passed in the late afternoon, between 15.00 and 18.00 hrs - 11,554 individuals (43.0% of all birds counted). The study confirmed that the front of Western Black Sea migration route, or "Via Pontica" as part of the Mediterranean/Black Sea Flyway in SE Bulgaria, is rather wide, since the magnitude of inland migration in Bourgas district can be compared with that along the Black Sea coast, at least during spring migration. Both observation areas qualify as Important Bird Areas (IBA) of European importance according to the several BirdLife International criteria. We recommend that any new wind farms built should avoid the area of Hisar Hill south of Karnobat and the NW parts of Sredets Municipality.
Fostering research skills in undergraduate medical students through Mentored Student Projects: Example from an Indian medical school
Authors: Devi V , Abraham R R , Adiga A , Ramnarayan K , Kamath A 
Published: June 2012

Background Healthcare decision-making is largely reliant on evidence–based medicine; building skills in scientific reasoning and thinking among medical students becomes an important part of medical education. Medical students in India have no formal path to becoming physicians, scientists or academicians. Objectives This study examines students’ perceptions regarding research skills improvement after participating in the Mentored Student Project programme at Melaka Manipal Medical College, Manipal Campus, India. Additionally, this paper describes the initiatives taken for the continual improvement of the Mentored Student Project programme based on faculty and student perspectives. Methods At Melaka Manipal Medical College, Mentored Student Project was implemented in the curriculum during second year of Bachelor of Medicine and Bachelor of Surgery programme with the intention of developing research skills essential to the career development of medical students. The study design was cross-sectional. To inculcate the spirit of team work students were grouped (n=3 to 5) and each group was asked to select a research project. The students’ research projects were guided by their mentors. A questionnaire (Likert’s five point scale) on students’ perceptions regarding improvement in research skills after undertaking projects and guidance received from the mentor was administered to medical students after they had completed their Mentored Student Project . The responses of students were summarised using percentages. The median grade with inter-quartile range was reported for each item in the questionnaire. The median grade for all the items related to perceptions regarding improvement in research skills was 4 which reflected that the majority of the students felt that Mentored Student Project had improved their research skills. The problems encountered by the students during Mentored Student Project were related to time management for the Mentored Student Project and mentors. Results This study shows that students acknowledged that their research skills were improved after participating in the Mentored Student Project programme. Conclusions The Mentored Student Project programme was successful in fostering positive attitudes among medical students towards scientific research. The present study also provides scope for further improvement of the Mentored Student Project programme based on students’ and faculty perspectives.DOI: http://dx.doi.org/10.3126/kumj.v8i3.6215 Kathmandu Univ Med J 2010;8(3):294-8
Sind Naturwaldreservate naturnäher als der Schweizer Wald?
Authors: Heiri Caroline , Brändli Urs-Beat , Bugmann Harald , Brang Peter 
Published: June 2012

Do strict forest reserves feature more naturalness than the Swiss forest?
In natural forest reserves (NFR), forests develop in the absence of direct human interference, primarily management. This increases their naturalness and should – in the long run – allow forests to regain primeval attributes. Based on stand structural data from the third National Forest Inventory (NFI) and 25 NFR in Switzerland, we investigate 1) whether NFR feature more naturalness than managed forests, 2) whether the naturalness of Swiss forests increases with the time elapsed since the cessation of management, and 3) to what extent NFR differ from Swiss forests that have not been managed during several decades. Thereby, we focus on the four main forest types beech, silver fir-beech, silver fir-Norway spruce and Norway spruce forests, and furthermore group the data according to the time since the last intervention (0–20, 21–50 and &gt; 50 years.). The structural features investigated differ only slightly between NFR and managed forest (0–20 years). NFR feature a higher abundance of “giant” trees (dbh ≥ 80 cm), higher stem numbers and greater basal area, and thus a higher growing stock. The abundance of snags, however, is indistinguishable between the two types of forests. Naturalness in Swiss forests increases only slightly with time since the last intervention, indicated by a higher abundance of small snags and a slightly higher number of giant trees. By contrast, clear differences can be seen between NFR in which no intervention has taken place for at least 50 years, and other forests abandoned for a similarly long time, with NFR showing lower stem numbers, greater basal area and higher numbers of giant trees. Many of the investigated reserves are still affected by former management and are – from the point of view of primeval forest dynamics – still quite “young”. According to their naturalness, the NFR range between managed and primeval forests, although to date they are still closer to the former. Nevertheless, the differences found in our study indicate a steady development of the NFR towards stand structures that are characteristic of primeval forests.
Old-growth forest reserves in Slovenia: the past, present, and future
Authors: Nagel Thomas A. , Diaci Jurij , Rozenbergar Dusan , Rugani Tihomir , Firm Dejan 
Published: June 2012

Old-growth forest reserves in Slovenia: the past, present, and future
Slovenia has a small number of old-growth forest remnants, as well as many forest reserves approaching old-growth conditions. In this paper, we describe some of the basic characteristics of these old-growth remnants and the history of their protection in Slovenia. We then trace the long-term development of research in these old-growth remnants, with a focus on methodological changes. We also review some of the recent findings from old-growth research in Slovenia and discuss future research needs. The conceptual understanding of how these forests work has slowly evolved, from thinking of them in terms of stable systems to more dynamic and unpredictable ones due to the influence of natural disturbances and indirect human influences. In accordance with this thinking, the methods used to study old-growth forests have changed from descriptions of stand structure to studies that address natural processes and ecosystem functions.