bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spirituality and job satisfaction among female Jewish Israeli hospital nurses
Authors: Lazar Aryeh 
Published: January 2010

lazar a. (2010) Spirituality and job satisfaction among female Jewish Israeli hospital nurses. Journal of Advanced Nursing 66(2), 334–344.AbstractTitle.  Spirituality and job satisfaction among female Jewish Israeli hospital nurses. Aim.  This paper is a report of a study conducted to examine the relationship between spirituality and hospital nurses’ work satisfaction and to determine the unique contributions of various specific aspects of spirituality to their work satisfaction.Background.  Spirituality has been indicated as a possible contributor to nurses’ job satisfaction. However, few researchers have examined the relationship between spirituality and nurses’ job satisfaction.Method.  During 2007, 120 female Jewish Israeli hospital nurses responded to a questionnaire including a multidimensional measure of spirituality and a measure of overall job satisfaction.Results.  Correlation analysis indicated a positive relationship between life coherency aspects of spirituality and spiritual values with job satisfaction. Hierarchical regression analysis indicated the particular importance of an idealistic spiritual orientation (positive contribution) and a transcendent spiritual orientation (negative contribution) to the prediction of nurses’ job satisfaction.Conclusion.  The relationship between spirituality and nurses’ job satisfaction is complex. Hospital managers may be able to enhance job satisfaction by providing opportunities for nurses to satisfy their spiritual needs (in particular, altruistic and ideological orientations) on the job. In addition, nurses with a strong transcendent orientation may be identified and given special attention to increase the likelihood of job satisfaction.
Time to precept: supportive and limiting conditions for precepting nurses.
Authors: Carlson Elisabeth , Pilhammar Ewa , Wann‐Hansson Christine 
Published: January 2010

carlson e., pilhammar e. & wann‐hansson c. (2010) Time to precept: supportive and limiting conditions for precepting nurses. Journal of Advanced Nursing66(2), 432–441.AbstractTitle.  Time to precept: supportive and limiting conditions for precepting nurses. Aim.  This paper is a report of a study describing conditions for precepting in a Swedish clinical context from the perspective of precepting nurses.Background.  Clinical practice is a complex part of nursing education, and registered nurses who are acting as preceptors for nursing students face a number of challenges that need to be addressed during the precepting process.Method.  An ethnographic approach guided by symbolic interactionism was used. Data were collected by participant observation and focus group interviews over a ten‐month period in 2006–2007. Participants were selected by purposive sampling of 13 staff nurses who were preceptors during the field work period. In addition, 16 staff nurses, experienced in precepting, were purposively selected for four focus groups.Findings.  Precepting was found to be a complex function for nurses, influenced by conditions that could be both supportive and limiting in nature. Three themes described these conditions: organization, comprising clinical responsibilities and routines; collaboration, focusing on professional relations and interactions; and the personal perspective, comprising preceptors’ experiences, need for feed back and notions of benefits. Time as a limiting condition reappeared through all categories.Conclusion.  It is important to raise the issue of time and its impact on the precepting process. Precepting needs to be further discussed in terms of an integrated nursing competence prioritized by all stakeholders involved in clinical practice. Therefore; efforts should be made to plan nurses’ clinical work so that allocated time for precepting can be facilitated.
Immigrant women’s experiences of receiving care in a mobile health clinic
Authors: Guruge Sepali , Hunter JoAnne , Barker Keegan , McNally Mary Jane , Magalhães Lilian 
Published: January 2010

guruge s., hunter j.a., barker k., mcnally m.j. & magalhães l. (2010) Immigrant women’s experiences of receiving care in a mobile health clinic. Journal of Advanced Nursing 66(2), 350–359.AbstractTitle.  Immigrant women’s experiences of receiving care in a mobile health clinic. Aim.  This paper is a report of a study of the experiences of Portuguese‐speaking immigrant women who used a mobile health clinic for their reproductive health care.Background.  Upon arrival in Canada, immigrant women often are in better health than their Canadian‐born counterparts; however, this health status tends to deteriorate over time. One reason for this change is limited access to services.Method.  Data collection during 2004 and 2005 involved individual interviews with seven Portuguese‐speaking women who received care in a mobile health clinic in Toronto, Canada, and with four clinic care providers. Non‐participant observation of the interaction between clients and care providers was also conducted. Interviews conducted in Portuguese were translated into English and transcribed, along with those conducted in English. Interview transcripts were read and re‐read in the context of observational notes to develop codes. Emerging codes were grouped together to develop subcategories and categories.Findings.  Participants’ experiences of accessing and receiving care in the mobile health clinic were shaped by their perceptions of health, which included physical, mental, social and spiritual aspects, and their pre‐ and postmigration care experiences. As an alternative model of care delivery, the mobile health clinic was perceived by participants to address their care needs and to help overcome postmigration barriers by providing accessible, holistic, and linguistically and culturally appropriate care.Conclusion.  Mobile health clinics should be considered as an alternative care delivery model for immigrant women who may be at a disadvantage because of their socio‐economic, cultural, and racialized statuses.
Generalizability and transferability of meta‐synthesis research findings
Authors: Finfgeld‐Connett Deborah 
Published: January 2010

finfgeld‐connett d. (2010) Generalizability and transferability of meta‐synthesis research findings. Journal of Advanced Nursing 66(2), 246–254.AbstractTitle.  Generalizability and transferability of meta‐synthesis research findings. Aim.  This paper is a report of a critical analysis of the generalizability and transferability of meta‐synthesis research findings.Background.  Findings from a large number of qualitative research investigations have had little impact on clinical practice and policy formation. Single qualitative investigations are not intended to produce findings that are directly applicable to clinical practice, simple literature reviews of qualitative studies are not conducted using sophisticated methods to develop new cumulative knowledge, and methods for systematically compiling and synthesizing qualitative findings have just recently been developed in nursing.Data sources.  This analysis of qualitative review methods was based on over 10 years of meta‐synthesis research experience and a non‐time‐limited cross‐discipline search of the English‐language literature related to qualitative research and generalizability.Discussion.  Generalizability of meta‐synthesis findings is enhanced by insuring validity through systematic sampling, second‐tier triangulation, maintenance of well‐documented audit trails and the development of multi‐dimensional theory. Generalizability of meta‐synthesis findings is tentative until successful transference to new situations takes place.Implications for nursing.  Nurse researchers are urged to conduct well‐designed and executed meta‐synthesis investigations that have the potential to generate findings that are relevant to clinical practice and policy formation. They are also encouraged to disseminate their meta‐synthesis findings skilfully and work with practitioners and policy‐makers to apply and evaluate them judiciously in clinical settings.Conclusion.  Qualitative meta‐synthesis is a way of putting together qualitative findings from disparate investigations so that they can more readily be used in clinical practice and policy formation.
Establishment and characterization of a fibroblast cell line derived from the dorsal fin of red sea bream, Pagrus major (Temminck & Schlegel)
Authors: Ku C‐C , Lu C‐H , Wang C‐S 
Published: January 2010

AbstractThe establishment and partial characterization of a continuous cell line from the dorsal fin of red sea bream, Pagrus major, are described. The cell line, designated RSBF‐2, has been subcultured for more than 100 passages since its initiation in November 2000. It was optimally maintained at 28 °C in Leibovitz L‐15 medium with 10% foetal bovine serum. Propagation of RSBF‐2 cells was serum dependent and exhibited low plating efficiency (<1.7%). Aside from long‐term cryopreservation, the cells could also be kept at 4 °C for 72 days. The distribution of the chromosome number was 38–98 with a mode of 48. The RSBF‐2 cell line was susceptible to red sea bream iridovirus but only produced a few rounded and refractory cells. Virus‐inoculated RSBF‐2 cells were then subcultured to generate a persistently infected cell line. RSBF‐2 was also very sensitive to the extracellular products of Photobacterium damselae ssp. piscicida and produced significant fluorescent signals after transfection with pEGFP‐C3. Analysis of mitochondrial cytochrome b gene sequences revealed 99% identity between the cell line and Pagrus major.
Susceptibility of koi × crucian carp and koi × goldfish hybrids to koi herpesvirus (KHV) and the development of KHV disease (KHVD)
Authors: Bergmann S M , Sadowski J , Kiełpiński M , Bartłomiejczyk M , Fichtner D , Riebe R , Lenk M , Kempter J 
Published: January 2010

AbstractHybrids of koi, Cyprinus carpio × crucian carp, Carassius carassius and koi × goldfish, Carassius auratus, proved to be susceptible to koi herpesvirus (KHV, syn. CyHV‐3) and developed KHV disease (KHVD). While hybrids of koi × goldfish were partly resistant to mortality following infection by immersion, most koi × crucian carp hybrids died after bath infection. KHV DNA was detected in dead fish but also in all surviving animals by different polymerase chain reactions (PCRs). According to these results, hybrid crossbreeding does not seem to prevent severe losses associated with KHV in terms of inducing KHVD. The present study showed severe losses after a waterborne KHV infection of between 35% and 100% in koi × goldfish and koi × crucian carp hybrids as well as in SPF carp.
Population genetic structure of Earth's largest fish, the whale shark (Rhincodon typus)
Authors: CASTRO A. L. F. , STEWART B. S. , WILSON S. G. , HUETER R. E. , MEEKAN M. G. , MOTTA P. J. , BOWEN B. W. , KARL S. A. 
Published: January 2010

AbstractLarge pelagic vertebrates pose special conservation challenges because their movements generally exceed the boundaries of any single jurisdiction. To assess the population structure of whale sharks (Rhincodon typus), we sequenced complete mitochondrial DNA control regions from individuals collected across a global distribution. We observed 51 single site polymorphisms and 8 regions with indels comprising 44 haplotypes in 70 individuals, with high haplotype (h = 0.974 ± 0.008) and nucleotide diversity (π = 0.011 ± 0.006). The control region has the largest length variation yet reported for an elasmobranch (1143–1332 bp). Phylogenetic analyses reveal no geographical clustering of lineages and the most common haplotype was distributed globally. The absence of population structure across the Indian and Pacific basins indicates that oceanic expanses and land barriers in Southeast Asia are not impediments to whale shark dispersal. We did, however, find significant haplotype frequency differences (amova, FST = 0.107, P < 0.001) principally between the Atlantic and Indo‐Pacific populations. In contrast to other recent surveys of globally distributed sharks, we find much less population subdivision and no evidence for cryptic evolutionary partitions. Discovery of the mating and pupping areas of whale sharks is key to further population genetic studies. The global pattern of shared haplotypes in whale sharks provides a compelling argument for development of broad international approaches for management and conservation of Earth's largest fish.
Self‐recruitment and sweepstakes reproduction amid extensive gene flow in a coral‐reef fish
Authors: CHRISTIE MARK R. , JOHNSON DARREN W. , STALLINGS CHRISTOPHER D. , HIXON MARK A. 
Published: January 2010

AbstractIdentifying patterns of larval dispersal within marine metapopulations is vital for effective fisheries management, appropriate marine reserve design, and conservation efforts. We employed genetic markers (microsatellites) to determine dispersal patterns in bicolour damselfish (Pomacentridae: Stegastes partitus). Tissue samples of 751 fish were collected in 2004 and 2005 from 11 sites encompassing the Exuma Sound, Bahamas. Bayesian parentage analysis identified two parent–offspring pairs, which is remarkable given the large population sizes and 28 day pelagic larval duration of bicolour damselfish. The two parent–offspring pairs directly documented self‐recruitment at the two northern‐most sites, one of which is a long‐established marine reserve. Principal coordinates analyses of pair‐wise relatedness values further indicated that self‐recruitment was common in all sampled populations. Nevertheless, measures of genetic differentiation (FST) and results from assignment methods suggested high levels of gene flow among populations. Comparisons of heterozygosity and relatedness among samples of adults and recruits indicated spatially and temporally independent sweepstakes events, whereby only a subset of adults successfully contribute to subsequent generations. These results indicate that self‐recruitment and sweepstakes reproduction are the predominant, ecologically‐relevant processes that shape patterns of larval dispersal in this system.
The determination of activity of the enzyme Rubisco in cell extracts of the dinoflagellate alga Symbiodinium sp. by manganese chemiluminescence and its response to short‐term thermal stress of the alga
Authors: LILLEY ROSS McC. , RALPH PETER J. , LARKUM ANTHONY W. D. 
Published: January 2010

ABSTRACTThe dinoflagellate alga Symbiodinium sp., living in symbiosis with corals, clams and other invertebrates, is a primary producer in coral reefs and other marine ecosystems. The function of the carbon‐fixing enzyme ribulose 1,5‐bisphosphate carboxylase/oxygenase (Rubisco) in dinoflagellates is difficult to study because its activity is rapidly lost after extraction from the cell. We report procedures for the extraction of Rubisco from Symbiodinium cells and for stable storage. We describe a continuous assay for Rubisco activity in these crude cell extracts using the Mn2+ chemiluminescence of Rubisco oxygenase. Chemiluminescence time courses exhibited initial transients resembling bacterial Form II Rubisco, followed by several minutes of linearly decreasing activity. The initial activity was determined from extrapolation of this linear section of the time course. The activity of fast‐frozen cell extracts was stable at −80 °C and, after thawing and storage on ice, remained stable for up to 1 h before declining non‐linearly. Crude cell extracts bound [14C] 2‐carboxy‐D‐arabitinol 1,5‐bisphosphate to a high molecular mass fraction separable by gel filtration chromatography. After pre‐treatment of Symbiodinium cell cultures in darkness at temperatures above 30 °C, the extracted Rubisco activities decreased, with almost complete loss of activity above 36 °C. The implications for the sensitivity to elevated temperature of Symbiodinium photosynthesis are assessed.
Founder effect of a pathogenic MSH2 mutation identified in Spanish families with Lynch syndrome
Authors: Menéndez M , Castellví‐Bel S , Pineda M , De Cid R , Muñoz J , González S , Teulé À , Balaguer F , Ramón y Cajal T , Reñé Josep M. , Blanco I , Castells A , Capellà G 
Published: January 2010

Menéndez M, Castellví‐Bel S, Pineda M, de Cid R, Muñoz J, González S, TeuléÀ, Balaguer F, Ramón y Cajal T, Maria Reñé J, Blanco I, Castells A, Capellà G. Founder effect of a pathogenic MSH2 mutation identified in Spanish families with Lynch syndrome.Lynch syndrome is caused by germline mutations in the mismatch repair genes MLH1, MSH2, MSH6 or PMS2. The novel MSH2 c.[2635‐3T>C; 2635‐5C>T] mutation was identified in 4 Lynch families, cosegregating with the disease. This mutation, located in intron 15, was predicted to alter the correct mRNA processing by in silico analysis.Our aim was to perform the c.[2635‐3T>C; 2635‐5C>T] mutation screening in high risk CRC cases and control populations, to evaluate the founder effect in our population by haplotype analysis and to confirm the pathogenic effect of the mutation by MSH2 expression studies.Mutation screening was performed by SSCP and CSCE in genomic DNA from 323 high risk CRC cases and 289 controls. Haplotyping was performed analysing 4 MSH2 extragenic microsatellite markers (D2S288, D2S2227, D2S1247 and D2S1248) in 50 controls and mutation carriers by using the PHASE program. We analysed the effect of the mutation in mRNA processing by RT‐PCR and in MSH2 expression by qRT‐PCR using RNA from 5 mutation carriers and 18 controls.None of the remaining high risk CRC cases or controls analysed harboured the mutation. We identified a common telomeric haplotype and two centromeric haplotypes, both rare in our population. Although we were not able to identify any abnormal transcript by RT‐PCR with the design used, we observed a significant reduction of mRNA MSH2 expression in carriers when compared with controls.Haplotype analyses suggest a founder effect of the c.[2635‐3T>C; 2635‐5C>T] MSH2 mutation and expression studies support a pathogenic role of this mutation.
Effects of Deficit Irrigation and Salinity Stress on Common Bean (Phaseolus Vulgaris L.) and Mungbean (Vigna Radiata (L.) Wilczek) Grown in a Controlled Environment
Authors: Bourgault M. , Madramootoo C. A. , Webber H. A. , Stulina G. , Horst M. G. , Smith D. L. 
Published: January 2010

AbstractAs water for irrigation purposes becomes increasingly scarce because of climate change and population growth, there is growing interest in regulated deficit irrigation (RDI) as a way to improve efficiency of water usage and farm productivity in arid and semi‐arid areas. Salinity is also becoming an important problem in these same regions. Experiments were performed to investigate the effects of RDI and salt stress on two legumes crops, common bean (Phaseolus vulgaris L.) and mungbean (Vigna radiata (L.) Wilczek); previous work showed contrasting responses to RDI by these two crops under field conditions. The seed and biomass yields of both crops were reduced as a result of increasing water deficit stress; however, mungbean was able to maintain the same proportion of its biomass in reproductive structures and maintain its harvest index under stress, whereas common bean’s decreased. In addition, photosynthesis in mungbean was higher than in common bean and higher at the same levels of transpiration. Finally, salinity stress did not affect the water potential, harvest index or the specific leaf weight of either crop. There were no interactions between salinity and crops or RDI levels, which suggest that the two crops do not differ in their response to salinity stress, and that RDI levels do not modify this response.
Sucrose Accumulation in Sugar Beet Under Drought Stress
Authors: Hoffmann C. M. 
Published: January 2010

AbstractDrought stress may affect sucrose accumulation of sugar beet by restricting leaf development and storage root growth. The objective of this study was to identify changes occurring in the storage root of Beta beets in growth characteristics and ions and compatible solutes accumulation under drought with regard to sucrose accumulation. Two pot experiments were conducted: (1) sugar beet well supplied with water (100 % water capacity), under continuous moderate (50 %) and severe drought stress (30 %), (2) sugar beet and fodder beet well supplied with water (100 %) and under continuous severe drought stress (30 %). Under drought stress, the ratio of storage root to leaf dry matter of sugar beet decreased indicating a different partitioning of the assimilates. The sucrose concentration of the storage root was reduced. In the root, the number of cambium rings was only slightly affected, although drought stress was implemented already 6 weeks after sowing. In contrast, the distance between adjacent rings and the cell size was considerably restricted, which points to a reduced expansion of existing sink tissues. The daily rate of sucrose accumulation in the root showed a maximum between 16 and 20 weeks after sowing in well‐watered plants, but it was considerably reduced under drought stress. The concentration of compatible solutes (K, Na, amino acids, glycine betaine, glucose and fructose) decreased during growth, while it was enhanced because of drought. However, when sucrose concentration was added, a constant sum of all examined solutes was found throughout the vegetation period. It was similar in sugar beet and in fodder beet despite different concentrations of single solutes, and the total sum was not affected by water supply. A close negative relationship between the concentration of compatible solutes and sucrose occurred. It is therefore concluded that the accumulation of compatible solutes in the storage root of Beta beets under drought might be a physiological constraint limiting sucrose accumulation.
Health‐related quality of life in diabetic patients with carpal tunnel syndrome
Authors: Thomsen N. O. B. , Cederlund R. , Björk J. , Dahlin L. B. 
Published: January 2010

Diabet. Med. 27, 466–472 (2010)AbstractAims  To determine health‐related quality of life (HRQL) in diabetic and non‐diabetic patients with carpal tunnel syndrome (CTS) before and after surgical treatment.Methods  In a prospective study, 35 consecutive diabetic patients with CTS were age and gender matched with 31 non‐diabetic patients with idiopathic CTS. At baseline (preoperatively), 6, 12 and 52 weeks after surgical carpal tunnel release, patients completed the generic Short‐Form 36 (SF‐36) and the disease‐specific Boston Carpal Tunnel Questionnaire (BCTQ).Results  The SF‐36 physical component scores at baseline were significantly reduced for diabetic (39 ± 7.4) compared with non‐diabetic patients (48 ± 9.0) (P < 0.05). Mixed model analysis demonstrated no differences in post‐surgical improvement over time between diabetic and non‐diabetic patients. The largest clinical effect was found for bodily pain (effect size 0.8). However, population norms were not reached for the diabetic patients. At baseline, no difference was found in mental component score, which deteriorated over time for diabetic patients. At baseline, BCTQ demonstrated that diabetic patients experienced more pronounced ‘numbness in the hand’ than non‐diabetic patients. Large clinical improvements were found in both symptom severity (effect size 1.98–2.14) and functional status score (effect size 0.89–0.94) for both diabetic and non‐diabetic patients, with no difference between the two patient groups.Conclusions  HRQL is impaired in diabetic patients with CTS compared with non‐diabetic patients with CTS and population norms. However, diabetic patients experience similar symptomatic and functional benefits from carpal tunnel release as do non‐diabetic patients.