bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of Walking Adaptability Training on Walking Capacity in Ambulatory People With Motor Incomplete Spinal Cord Injury: A Multicenter Pragmatic Randomized Controlled Trial
Authors: Zwijgers Eline , van Dijsseldonk Rosanne B. , Vos-van der Hulst Marije , Hijmans Juha M. , Geurts Alexander C. H. , Keijsers Noël L. W. 
Published: April 2024

Background and Objective Balance and walking capacity are often impaired in people with motor incomplete spinal cord injury (iSCI), frequently resulting in reduced functional ambulation and participation. This study aimed to assess the efficacy of walking adaptability training compared to similarly dosed conventional locomotor and strength training for improving walking capacity, functional ambulation, balance confidence, and participation in ambulatory people with iSCI. Methods We conducted a 2-center, parallel-group, pragmatic randomized controlled trial. Forty-one people with iSCI were randomized to 6 weeks of (i) walking adaptability training (11 hours of Gait Real-time Analysis Interactive Lab (GRAIL) training—a treadmill in a virtual reality environment) or (ii) conventional locomotor and strength training (11 hours of treadmill training and lower-body strength exercises). The primary measure of walking capacity was maximal walking speed, measured with an overground 2-minute walk test. Secondary outcome measures included the Spinal Cord Injury Functional Ambulation Profile (SCI-FAP), the Activities-specific Balance Confidence (ABC) scale, and the Utrecht Scale for Evaluation of Rehabilitation-Participation (USER-P). Results No significant difference in maximal walking speed between the walking adaptability (n = 17) and conventional locomotor and strength (n = 18) training groups was found 6 weeks after training at follow-up (−0.05 m/s; 95% CI = −0.12-0.03). In addition, no significant group differences in secondary outcomes were found. However, independent of intervention, significant improvements over time were found for maximal walking speed, SCI-FAP, ABC, and USER-P restrictions scores. Conclusions. Our findings suggest that walking adaptability training may not be superior to conventional locomotor and strength training for improving walking capacity, functional ambulation, balance confidence, or participation in ambulatory people with iSCI. Trial Registration Dutch Trial Register; Effect of GRAIL training in iSCI.
MMP-1, MMP-8, and MMP-13 Gene Polymorphisms and Haplotype Is a Risk Factor for Dupuytren Contracture: A Case-Control Study
Authors: Rodrigues Mauricio P. , Tissi Larissa H. , Oliveira Vinicius M. , Wistuba Guilherme A. S. M. , Araujo Francielle B. , Mattar-Júnior Rames , Rezende Marcelo R. , Wei Teng H. , Godoy-Santos Alexandre L. , Santos Maria Cristina L. G. 
Published: April 2024

Background: There is an increased tissue expression of matrix metalloproteinases (MMPs) on Dupuytren contracture (DC). Genetic polymorphisms (single nucleotide polymorphism [SNPs]) in genes of these enzymes may individually influence these transcriptions. Haplotype analysis, which is the observation of a group of alleles, could be more useful to identify the association between SNPs and DC. The purpose of this study was to evaluate the influence of MMP-1 g.-1607 G>GG (rs1799750), MMP-8 g.-799 C>T (rs11225395), and MMP-13 g.-77 A>G (rs2252070) SNPs individually and in haplotype on DC. Methods: A total of 60 patients with a clinical diagnosis of DC were evaluated and matched, according to age and gender, with the control group of 100 patients without this clinical diagnosis. Genomic DNA was extracted from saliva samples, and genotypes were obtained by polymerase chain reaction-restriction fragment length polymorphism. Statistical analysis of the results included Mann-Whitney U test, Chi-squared test, and PHASE and R software, with a significance level of 5%. Results: The 3 SNPs studied showed significant differences in allele and genotype frequencies between the groups: 2G in MMP-1 ( P = .018; odds ratio [OR] 1.80 (95% confidence interval [CI], 1.13-2.88)), T in MMP-8 ( P = .015; OR 0.53 (95% CI, 0.33-0.88)), and A in MMP-13 (rs2252070) SNPs ( P = .040, OR 0.54 (95% CI, 0.33-0.90)) are risk alleles. The global haplotype analysis indicated a significant difference between both groups. Conclusions: In conclusion, MMP-1 g.-1607 G>GG (rs1799750), MMP-8 g.-799 C>T (rs11225395), and MMP-13 g.-77 A>G (rs2252070) SNPs, individually and in haplotype, are a risk factor for DC, indicating that these SNPs may be a potential diagnostic and prognostic factor for DC.
Optimal flow of high-flow nasal cannula oxygenation to prevent desaturation during sedation for bronchoscopy: a randomized controlled study
Authors: Zhang Wen , Yuan Xiaohong , Shen Yajian , Wang Jiangling , Xie Kangjie , Chen Xinzhong 
Published: April 2024

Background: Although high-flow nasal cannula (HFNC) oxygenation is currently recommended to prevent desaturation during sedation for bronchoscopy, there is no consensus on an optimal flow rate. Objective: To determine the optimal oxygen flow rate for HFNC to effectively prevent desaturation during sedation for bronchoscopy. Design: Prospective, randomized, and controlled study. Methods: Patients ( n = 240) scheduled for bronchoscopy were randomized to receive HFNC with propofol sedation (fraction of inspired oxygen, 100%) at one of six flow rates of 10, 20, 30, 40, 50, and 60 L/min, designated as groups 1–6, respectively. Results: The incidence of desaturation significantly decreased by increasing the oxygen flow rate (42.5%, 17.5%, 15%, 10%, 2.5%, and 0% for groups 1–6, respectively, p < 0.0001). The optimal oxygen flow rate for HFNC determined by probit regression to effectively prevent desaturation in 95% of patients was 43.20 (95% confidence interval, 36.43–55.96) L/min. The requirement for airway intervention was significantly decreased by increasing the oxygen flow rate. Conclusion: An HFNC flow rate of 50–60 L/min is recommended to prevent desaturation during sedation for bronchoscopy. Registration: NCT05298319 at ClinicalTrials.gov.
Risk Factors of Serious Adverse Events for Geriatric Hip Fractures: Is it the Frailty or the Timing?
Authors: Wang Ling-xiao , Guan Li-juan , Deng Ming-hong , Zhou Li-hua 
Published: April 2024

Objective Preoperative frailty and surgical waiting times are associated with the occurrence of adverse outcomes in patients with hip fractures. Specifically, we aimed to investigate the influence of frailty status and surgical timing on the risk of serious adverse events during hospitalization. Methods This study utilized an observational single cohort design and included patients aged ≥60 years with a primary diagnosis of hip fracture. Frailty was assessed using the chart-derived frailty index (CFI), which was calculated based on demographic and routine laboratory variables. The primary outcome of interest was the occurrence of in-hospital serious adverse events. A multivariate logistic regression model was utilized to examine the risk factors influencing outcomes. Results The study included 427 participants, with a mean age of 80.28 ± 8.13 years and 64.2% of whom were female. Patients with high CFI have more comorbidities ( P < .001), lower surgical rates ( P = .002), and delayed surgical times ( P = .033). A total of 239 patients (56.0%) experienced serious adverse events. The high CFI group had a significantly higher occurrence of serious adverse events compared to the low CFI group (73.4% vs 48.5%, P < .001). After adjusting for surgical timing and covariates, the multivariate logistic regression analysis revealed that high frailty significantly increased the risk for serious adverse events (OR = 2.47, 95% CI 1.398-4.412), infection (OR = 1.99, 95% CI 1.146-3.446), acute heart failure (OR = 3.37, 95% CI 1.607-7.045). However, the timing of surgery did not demonstrate any association with these outcomes. In addition, after adjusting for surgical factors, high CFI remains an independent risk factor for these complications. Conclusions Frailty serves as a reliable predictor of the probability of encountering severe adverse events while hospitalized for elderly individuals with hip fractures. This method has the potential to pinpoint particular modifiable factors that necessitate intervention, whereas the impact of surgical timing remains uncertain and necessitates additional research.
Kvačekite, NiSbSe, a new selenide mineral from Bukov, Czech Republic
Authors: Pauliš Petr , Dolníček Zdeněk , Sejkora Jiří , Pour Ondřej , Laufek František , Ulmanová Jana , Vymazalová Anna 
Published: April 2024

AbstractKvačekite is a new mineral species discovered in a sample collected from the now abandoned Bukov uranium mine, western Moravia, Czech Republic. It occurs as rare anhedral grains, up to 15 μm in size, associated with nickeltyrrellite, tyrrellite, berzelianite, hakite-(Zn), hakite-(Cd), eucairite, clausthalite, and gold in calcite gangue. In reflected light, kvačekite is white with a faint yellowish shade; bireflectance, pleochroism and anisotropy are absent. Internal reflections were not observed. Reflectance values for the four COM wavelengths for kvačekite in air [R (%) (λ in nm)] are: 54.9 (470); 53.5 (546); 52.6 (589); and 52.2 (650). The empirical formula, based on electron-microprobe analyses (EPMA), is (Ni0.95Cu0.04Co0.03)Σ1.02Sb1.00(Se0.97S0.01)Σ0.98. The ideal formula is NiSbSe, which requires (in wt.%) Ni 22.63, Sb 46.93, Se 30.44, total 100.00. Kvačekite is cubic, P213, with unit-cell parameters a = 6.09013(13) Å, V = 225.881(15) Å3 and Z = 4. The strongest reflections in the X-ray powder diffraction pattern of synthetic kvačekite [d, Å (I) hkl] are: 3.0458 (11) 200; 2.7242 (100) 201, 210; 2.4867 (71) 211; 1.8632(39) 311; 1.6277(29) 321, 312; and 1.3290 (13) 421. Given the similarity with ullmannite, NiSbS, the crystal structure was refined from the powder X-ray diffraction data starting from those atomic coordinates using the synthetic analogue of kvačekite. Its crystal structure is formed by corner-sharing [NiSb3Se3] octahedra which form a three-dimensional network. The identity of the natural kvačekite and synthetic cubic NiSbSe were confirmed by a study of their chemical composition, reflectance measurements, Raman spectroscopy and electron back-scattered diffraction (EBSD) measurements on the mineral. Kvačekite is named after Milan Kvaček (1930–1993), a prominent Czech mineralogist. The mineral and its name have been approved by the Commission on New Minerals, Nomenclature and Classification of the International Mineralogical Association (IMA2023-095).
Ermeloite, AlPO4⋅H2O a new phosphate mineral with kieserite-type structure from Galicia, Spain
Authors: Vérez Guillermo Z. , Vázquez Carlos J. Rodríguez , Mariño Bruno Dacuña , Cereijo Inés Fernández , del Tánago José González , Martínez Ramón Jiménez , Pérez Ramiro Barreiro , Segurado Raquel Antón , Fernández Ezequiel Vázquez , Dopazo Montse Gómez , Pazos Aida González , Lantes-Suárez Oscar 
Published: April 2024

AbstractErmeloite is a new aluminium phosphate mineral from Galicia, Spain, in the northwest of the Iberian Peninsula. It is the third formally recognised mineral discovered in Galicia since morenosite and cervantite in the 19th Century. The name and symbol (Erm) were approved by the Commission on New Minerals, Nomenclature and Classification of the International Mineralogical Association (IMA2021–017a) in recognition of the geographical location where it was found. The mineral occurs as a light blue to white fine aggregate over quartz and microcline associated with wardite. Crystals of ~0.04 mm are transparent and have a waxy lustre. The simplified empirical formula determined using electron microprobe analysis is Al1.02P0.95F0.06O3.88⋅1.06 H2O, which is near to the ideal formula Al(PO4)⋅H2O. The mineral is an alteration product within a phosphate pegmatite. Ermeloite is the second phosphate isostructural with the sulfates of the kieserite group. Single-crystal X-ray diffraction showed that ermeloite crystallises in the monoclinic C2/c space group with cell parameters a = 6.5371(4) Å, b = 7.5670(5) Å, c = 7.1146(5) Å, β = 115.335(2)°, V = 318.08(4) Å3 and Z = 4 at room temperature. Comparative analysis of the crystallographic data, with isostructural phosphates, revealed an interesting behaviour for these compounds.
Symptom burden and its associations with clinical characteristics in patients with COPD: a clustering approach
Authors: Houben-Wilke Sarah , Deng Qichen , Janssen Daisy J.A. , Franssen Frits M.E. , Spruit Martijn A. 
Published: April 2024

BackgroundSymptom burden in patients with COPD is often under-recognised. In this cross-sectional analysis, we aimed to study the severity of a variety of (non-)respiratory symptoms in patients with and without COPD and to explore the associations between clusters based on symptom severity and other clinical characteristics.MethodsCharacteristics were assessed in 538 patients with COPD from primary, secondary and tertiary care and 116 non-COPD participants. The severity of 20 symptoms was measured using a visual analogue scale (VAS), ranging from 0 mm (no symptom) to 100 mm (maximum severity). K-means cluster analysis was applied to symptom severity in the patient sample only.ResultsPeople with COPD were comparable with non-COPD participants in terms of gender (58%versus55% male, p=0.132) and age (64±9 yearsversus63±6 years, p=0.552) and had a reduced forced expiratory volume in 1 s (57±23% predictedversus111±17% predicted, p<0.001). The COPD group had higher VAS scores for most symptoms (p<0.05). The most severe symptoms in patients with COPD were dyspnoea, fatigue and muscle weakness while non-COPD participants mainly experienced insomnia and micturition. Three clusters were identified in the patient sample. Health status and care dependency differed between all clusters, while functional mobility, exacerbation history and lung function differed between cluster 1 and the other two clusters (p<0.05).ConclusionsPeople with COPD report a high burden of respiratory as well as non-respiratory symptoms. Cluster analysis demonstrated a co-occurrence of different levels of symptom severity, highlighting the heterogeneity of symptoms experience. Identifying clusters of patients with shared symptom experiences will help us to understand the impact of the disease and define integrated, multidimensional treatment strategies.
Environmental significance of the interaction between titanium dioxides and soil solutions
Authors: Solymos Karolina , Babcsányi Izabella , Ariya Badam , Gyulavári Tamás , Ágoston Áron , Kukovecz Ákos , Kónya Zoltán , Pap Zsolt 
Published: April 2024

AbstractNanotechnology, especially in the field of photocatalysis, has witnessed rapid advancements, with titanium dioxide being one of the most widely used photocatalysts. As the use of products containing photoactive nanomaterials increases, concerns have arisen regarding their potential release into the environment over time. This release can impact soil, groundwater, and surrounding ecosystems, resulting in nanoparticles being dispersed in water and eventually depleted from the system. This study aimed to investigate how different soil solutions affect the structural, textural properties, and photocatalytic activity of titanium dioxide-based, commercial reference Evonik Aeroxide P25. The Regosol soil solution, characterized by acidic pH, low ionic content, and high organic matter content, induced nanoparticle aggregation and bandgap changes. In addition, the acidic pH hindered the adsorption process, potentially affecting the photocatalytic processes. In contrast, the Chernozem soil solution, with slightly alkaline pH, high ionic content, and low organic matter content, did not significantly alter the morphology or structure of the material. However, various organic compounds were absorbed on the surface, reducing the availability of active sites. The study highlights the importance of understanding the influence of soil solutions on nanomaterials, as it impacts their properties and environmental risks. Results show that the material is still activated, i.e., it can exert its photoactive effect on the environment. This sheds light on the challenges posed by nanoparticles in soil, particularly in terms of their toxicity and consequences for the surrounding ecosystems. The study underlines the need for further research in this area to assess potential risks and optimise the use of nanomaterials in environmental remediation.
Fast Track Adaptation of Oncolytic Coxsackie B3 Virus to Resistant Colorectal Cancer Cells - a Method to Personalize Virotherapy
Authors: Elsner Leslie , Heimann Lisanne , Geisler Anja , Dieringer Babette , Knoch Klaus-Peter , Hinze Luisa , Klingel Karin , Solimena Michel , Kurreck Jens , Fechner Henry 
Published: April 2024

Abstract
Background
The efficacy of oncolytic viruses (OV) in cancer treatment depends on their ability to successfully infect and destroy tumor cells. However, patients’ tumors vary, and in the case of individual insensitivity to an OV, therapeutic efficacy is limited. Here, we present a protocol for rapid generation of tumor cell-specific adapted oncolytic coxsackievirus B3 (CVB3) with enhanced oncolytic potential and a satisfactory safety profile. This is achieved by combining directed viral evolution (DVE) with genetic modification of the viral genome and the use of a microRNA-dependent regulatory tool.

Methods
The oncolytic CVB3 variant PD-H was adapted to the refractory colorectal carcinoma cell line Colo320 through serial passaging. XTT assays and virus plaque assays were used to determine virus cytotoxicity and virus replication in vitro. Recombinant PD-H variants were generated through virus mutagenesis. Apoptosis was detected by Western blots, Caspase 3/7 assays, and DAPI staining. The therapeutic efficacy and safety of the adapted recombinant OV PD-SK-375TS were assessed in vivo using a subcutaneous Colo320 xenograft mouse model.

Results
PD-H was adapted to the colorectal cancer cell line Colo320 within 10 passages. Sequencing of passage 10 virus P-10 revealed a heterogenous virus population with five nucleotide mutations resulting in amino acid substitutions. The genotypically homogeneous OV PD-SK was generated by inserting the five detected mutations of P-10 into the genome of PD-H. PD-SK showed significantly stronger replication and cytotoxicity than PD-H in Colo320 cells, but not in other colorectal carcinoma cell lines. Increase of apoptosis induction was detected as key mechanisms of Colo320 cell-specific adaptation of PD-SK. For in vivo safety PD-SK was engineered with target sites of the miR-375 (miR-375TS) to exclude virus replication in normal tissues. PD-SK-375TS, unlike the PD-H-375TS not adapted homolog suppressed the growth of subcutaneous Colo320 tumors in nude mice without causing any side effects.

Conclusion
Taken together, here we present an optimized protocol for the rapid generation of tumor cell-specific adapted oncolytic CVB3 based on the oncolytic CVB3 strain PD-H. The protocol is promising for the generation of personalized OV for tumor therapy and has the potential to be applied to other OV.
Survival of highly related ESBL- and pAmpC- producing Escherichia coli in broiler farms identified before and after cleaning and disinfection using cgMLST
Authors: Robé Caroline , Projahn Michaela , Boll Katrin , Blasse Anja , Merle Roswitha , Roesler Uwe , Friese Anika 
Published: April 2024

Abstract
Background
Broiler chickens are frequently colonized with Extended-Spectrum Beta-Lactamase- (ESBL-) and plasmid mediated AmpC Beta-Lactamase- (pAmpC-) producing Enterobacterales, and we are confronted with the potential spread of these resistant bacteria in the food chain, in the environment, and to humans. Research focused on identifying of transmission routes and investigating potential intervention measures against ESBL- and pAmpC- producing bacteria in the broiler production chain. However, few data are available on the effects of cleaning and disinfection (C&D) procedures in broiler stables on ESBL- and pAmpC- producing bacteria.

Results
We systematically investigated five broiler stables before and after C&D and identified potential ESBL- and pAmpC- colonization sites after C&D in the broiler stables, including the anteroom and the nearby surrounding environment of the broiler stables. Phenotypically resistant E. coli isolates grown on MacConkey agar with cefotaxime were further analyzed for their beta-lactam resistance genes and phylogenetic groups, as well as the relation of isolates from the investigated stables before and after C&D by whole genome sequencing. Survival of ESBL- and pAmpC- producing E. coli is highly likely at sites where C&D was not performed or where insufficient cleaning was performed prior to disinfection. For the first time, we showed highly related ESBL-/pAmpC- producing E. coli isolates detected before and after C&D in four of five broiler stables examined with cgMLST. Survival of resistant isolates in investigated broiler stables as well as transmission of resistant isolates from broiler stables to the anteroom and surrounding environment and between broiler farms was shown. In addition, enterococci (frequently utilized to detect fecal contamination and for C&D control) can be used as an indicator bacterium for the detection of ESBL-/pAmpC- E. coli after C&D.

Conclusion
We conclude that C&D can reduce ESBL-/pAmpC- producing E. coli in conventional broiler stables, but complete ESBL- and pAmpC- elimination does not seem to be possible in practice as several factors influence the C&D outcome (e.g. broiler stable condition, ESBL-/pAmpC- status prior to C&D, C&D procedures used, and biosecurity measures on the farm). A multifactorial approach, combining various hygiene- and management measures, is needed to reduce ESBL-/pAmpC- E. coli in broiler farms.
Detection of blaKPC gene among carbapenemase producing Klebsiella pneumoniae isolated from different clinical specimens at tertiary care hospital of Nepal
Authors: Baral Rakshya , Tuladhar Reshma , Manandhar Sarita , Singh Anjana , Sherchan Samendra 
Published: April 2024

Abstract
Background
Klebsiella pneumoniae infections have become a major cause of hospital acquired infection worldwide with the increased rate of acquisition of resistance to antibiotics. Carbapenem resistance mainly among Gram negative is an ongoing problem which causes serious outbreaks dramatically limiting treatment options. This prospective cross-sectional study was designed to detect blaKPC gene from carbapenem resistant K. pneumoniae.

Materials and Methods
A totally of 1118 different clinical specimens were screened and confirmed for KPC producing K. pneumoniae phenotypically using Meropenem (10 μg) disc. The blaKPC gene was amplified from the isolates of K. pneumoniae to detect the presence of this gene.

Result
Of the total samples processed, 18.6% (n = 36) were K. pneumoniae and among 36 K. pneumoniae, 61.1% (n = 22/36) were meropenem resistant. This study demonstrated the higher level of MDR 91.7% (n = 33) and KPC production 47.2% (n = 17) among K. pneumoniae isolates. The blaKPC gene was detected in 8.3% (n = 3) of meropenem resistant isolates.

Conclusion
Since the study demonstrates the higher level of MDR and KPC producing K. pneumoniae isolates that has challenged the use of antimicrobial agents, continuous microbiology, and molecular surveillance to assist early detection and minimize the further dissemination of blaKPC should be initiated. We anticipate that the findings of this study will be useful in understanding the prevalence of KPC-producing K. pneumoniae in Nepal.