bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increase in circulating levels of IGF‐1 and IGF‐1/IGFBP‐3 molar ratio over a decade is associated with colorectal adenomatous polyps
Authors: Soubry Adelheid , Il'yasova Dora , Sedjo Rebecca , Wang Frances , Byers Tim , Rosen Clifford , Yashin Anatoli , Ukraintseva Svetlana , Haffner Steven , D'Agostino Ralph 
Published: August 2011

AbstractHigh levels of circulating insulin‐like growth factor‐1 (IGF‐1) have been associated with increased risk of several cancers. Regarding colorectal cancer, these associations are generally weak. We hypothesized that an increase in IGF‐1 over time would be a stronger risk factor for cancer‐related outcomes than the actual levels. In this analysis we utilized existing data from the Insulin Resistance and Atherosclerosis Study (IRAS). Circulating IGF‐1 levels and molar ratios of IGF‐1 to IGF binding protein 3 (IGFBP‐3) were measured at three time points, within a 10‐year follow‐up period. We examined the associations of increase of the two variables with the presence of colorectal adenoma at the end of follow‐up among participants with normal glucose tolerance at baseline. This included 143 individuals, from which 24 were diagnosed with adenomatous polyps. Although the mean levels of IGF‐1 and IGF‐1/IGFBP‐3 decline with age, ∼ 30% of the participants showed an increase of at least fifteen percent (“ever increase”) in one or both of these variables, compared to baseline. We found a positive association between “ever increase” in IGF‐1 or IGF‐1/IGFBP‐3 and the presence of colorectal adenoma: ORs were 3.81 (95% CI: 1.30–10.8) and 2.83 (95% CI: 1.00–8.22), respectively. No association was found when analyzing the actual levels of both variables at any time point. Our data suggest that an increase in circulating IGF‐1 or IGF‐1/IGFBP‐3 may represent a disturbed GH/IGF1 homeostasis, which could favor the development of precancerous lesions such as colorectal adenoma.
Dimeric Resorcin[4]arene Capsules in the Solid State
Authors: Beyeh N. Kodiah , Rissanen Kari 
Published: August 2011

AbstractSupramolecular chemistry research is focused on the study of weak non‐covalent intermolecular — that is, supramolecular — interactions as the driving force in self‐assembly and molecular recognition. Dimeric resorcin[4]arenes capsules have been a focus of our research for the last 15 years. This review describes the solid state complexation studies of unsubstituted phenolic resorcin[4]arenes and pyrogall[4]arenes towards the formation of dimeric capsules and assemblies using ionic and neutral species as guest molecules and templates. The multitude of different crystal structures obtained during these studies demonstrates the versatile nature of resorcin[4]arenes and pyrogall[4]arenes (2‐hydroxy‐resorcin[4]arenes) as supramolecular hosts in crystal engineering.
Effect of surface chemistry on bacterial adhesion, viability, and morphology
Authors: Parreira Paula , Magalhães Ana , Gonçalves Inês C. , Gomes Joana , Vidal Ricardo , Reis Celso A. , Leckband Deborah E. , Martins M. Cristina L. 
Published: August 2011

AbstractHelicobacter pylori (H. pylori) is one of the most common infectious agents in the world and it is thought to colonize the gastric mucosa of about half of the world's population causing several gastric diseases. In this work, the effect of surface chemistry on H. pylori nonspecific adhesion, viability, and morphology was evaluated using three H. pylori strains with different adhesins expression profile. Self‐assembled monolayers (SAMs) of alkanethiols on gold were used to obtain surfaces exposing different functional groups: OH, CH3, and ethylene glycol (EG4). Bacterial adhesion onto the surfaces reached a plateau at 2 h. There was a correlation between adhesion and the exposed surface group, with bacterial cells adhering preferentially to CH3‐SAMs while EG4‐SAMs prevented H. pylori adhesion during the entire adhesion test (24 h). Surfaces that presented the EG4 group were also the only ones that significantly reduced the viability of adhered bacteria. Surface chemistry also influenced the morphology of adhered bacteria. The H. pylori rod shape observed in the control (Tissue Culture Polyethylene—TCPE) was only retrieved on CH3‐SAMs. This work demonstrates that surface chemistry, namely specific functional groups on the material, influence the nonspecific adsorption of H. pylori. Moreover, the features of the bacterial strain and the surface chemistry can alter the adhesion kinetics, as well as the morphology and viability of attached bacteria. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
In vitro effect on cancer cells: Synthesis and preparation of polyurethane membranes for controlled delivery of curcumin
Authors: Nagarajan Selvaraj , Reddy Bo Siva Rami , Tsibouklis Jhon 
Published: August 2011

AbstractUrethane polymers (PU) have been prepared from low‐molecular weight polylactic acid (PLA) and hexamethylene diisocyanate (HMDI) using polydimethylsiloxane (PDMS) as a chain extender. These formed the supporting polymeric matrix of curcumin‐containing PU membranes which were prepared using a solvent evaporation technique. FTIR and XRD data indicated the molecular‐level dispersion and random distribution of curcumin in the polymer matrix, and data were consistent with observations from tensile‐strength measurements and from AFM imaging. Determination of water vapor permeability and moisture uptake measurements have indicated that the PU membrane were appropriate for use on human skin. Skin permeation studies of curcumin were consistent with zero order (R2 = 0.9874) and with Korsmeyer‐Peppas (R2 = 0.9978) kinetics‐analytical data pointed to permeation by a combination of diffusion and erosion processes, with the latter dominating. The biocompatibility of these PU membranes was indicated by in vitro cytotoxicity studies using 3T3‐L1‐Murine fibroblast cell. The in vitro therapeutic potential of the patches was demonstrated against A549 human Lung cancer cells. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
The effect of calcium phosphate bone substitute on defect resolution around a rough‐surfaced dental implants in dogs
Authors: Kim Sungtae , Jung Ui‐Won , Lee Yong‐Keun , Choi Seong‐Ho 
Published: August 2011

AbstractGap defects often exist around dental implants due to morphological differences between the natural tooth extraction socket and the dental implant. Techniques that can resolve such gap defects include implant surface modification and filling of the defects with bone substitutes. Modified surfaces are generally more effective in this regard than smooth surfaces. Favorable results have also been reported using bone substitutes. This study evaluated the effectiveness of a calcium phosphate (CaP) bone substitute for resolving gap defects around implant surfaces that have been treated with grit blasting and thermal etching. Implants were placed in edentulous areas in four mongrel dogs. Gap defects with a diameter of 2 mm were prepared surgically around the dental implants. These defects were either filled with CaP bone substitute (experimental group) or left unfilled (control group). Defects were evaluated after 8 and 16 weeks of healing. Block specimens were fixed, sectioned, and stained with hematoxylin and eosin. Histometric measurements revealed that healing in gap defects that had been filled with CaP bone substitute proceeded until 16 weeks. Total CaP degradation seemed to occur at between 4 and 8 weeks of healing. In conclusion, a more complete defect resolution was observed in gap defects filled with CaP bone substitute after 16 weeks than after 8 weeks of healing. The beneficial effects of filling in 2‐mm gap defects around implants were attributed to the use of CaP bone substitute. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.
Oophorectomy‐induced bone loss is attenuated in MAGP1‐deficient mice
Authors: Craft Clarissa S. , Broekelmann Thomas J. , Zou Wei , Chappel Jean C. , Teitelbaum Steven L. , Mecham Robert P. 
Published: August 2011

AbstractMicrofibril‐associated glycoprotein‐1 (MAGP1), together with the fibrillins, are constitutive components of vertebrate microfibrils. Mice deficient in MAGP1 (murine MAGP1 knockout animals (Mfap2−/−); MAGP1Δ) is appropriate develop progressive osteopenia and reduced whole bone strength, and have elevated numbers of osteoclasts lining the bone surface. Our previous studies suggested that the increased osteoclast population was associated with elevated levels of receptor activator of NF‐κB ligand (RANKL), a positive regulator of osteoclast differentiation. To explore the relationship between RANKL expression and osteoclast differentiation in MAGP1 deficiency, oophorectomy (OVX) was used to stimulate RANKL expression in both WT and MAGP1Δ animals. Bone loss following OVX was monitored using whole body DEXA and in vivo µCT. While WT mice exhibited significant bone loss following OVX, percent bone loss was reduced in MAGP1Δ mice. Further, serum RANKL levels rose significantly in OVX WT mice, whereas, there was only a modest increase in RANKL following OVX in the mutant mice due to already high baseline levels. Elevated RANKL expression was normalized when cultured MAGP1Δ osteoblasts were treated with a neutralizing antibody targeting free TGFβ. These studies provide support for increased RANKL expression associated with MAGP1 deficiency and provide a link to altered TGF‐β signaling as a possible causative signaling pathway regulating RANKL expression in MAGP1Δ osteoblasts. J. Cell. Biochem. 113: 93–99, 2012. © 2011 Wiley Periodicals, Inc.
The box a domain of high mobility group box‐1 protein as an efficient siRNA carrier with anti‐inflammatory effects
Authors: Lee Sanghyun , Song Hojung , Kim Hyun Ah , Oh Binna , Lee Dong Yun , Lee Minhyung 
Published: August 2011

AbstractHigh mobility group box‐1 (HMGB‐1) is a DNA binding nuclear protein and pro‐inflammatory cytokine. The box A domain of HMGB‐1 (rHMGB‐1A) exerts an anti‐inflammatory effect, inhibiting wild‐type HMGB‐1 (wtHMGB‐1). In this study, HMGB‐1A was evaluated as an siRNA carrier with anti‐inflammatory effects. HMGB‐1A was expressed and purified by consecutive nickel chelate chromatography, cationic exchange chromatography, and polymixin B chromatography. Purified rHMGB‐1A demonstrated an anti‐inflammatory effect, reducing tumor necrosis factor‐α (TNF‐α) in wtHMGB‐1 or lipopolysaccharide (LPS) activated macrophages. In gel retardation assay, rHMGB‐1A formed a stable complex with siRNA at or above a 1:2 weight ratio (siRNA:rHMGB‐1A). A heparin competition assay showed that an siRNA/rHMGB‐1A complex released siRNA more easily than an siRNA/polyethylenimine (PEI, 25 kDa) complex. Luciferase siRNA/rHMGB‐1A reduced firefly luciferase expression at a similar level as luciferase siRNA/PEI complex. Furthermore, TNF‐α siRNA/rHMGB‐1A synergistically reduced TNF‐α expression in LPS activated macrophages. Therefore, rHMGB‐1A may be useful as an siRNA carrier with anti‐inflammatory effects in siRNA therapy for various inflammatory diseases. J. Cell. Biochem. 113: 122–131, 2012. © 2011 Wiley Periodicals, Inc.
Estrogen Controls embryonic stem cell proliferation via store‐operated calcium entry and the nuclear factor of activated T‐cells (NFAT)
Authors: Wong Chun‐Kit , So Wing‐Yan , Law Sau‐Kwan , Leung Fung‐Ping , Yau Ka‐Long , Yao Xiaoqiang , Huang Yu , Li Xiangdong , Tsang Suk‐Ying 
Published: August 2011

AbstractEmbryonic stem cells (ESCs) can self‐renew indefinitely and differentiate into all cell lineages. Calcium is a universal second messenger which regulates a number of cellular pathways. Previous studies showed that store‐operated calcium channels (SOCCs) but not voltage‐operated calcium channels are present in mouse ESCs (mESCs). In this study, store‐operated calcium entry (SOCE) was found to exist in mESCs using confocal microscopy. SOCC blockers lanthanum, 2‐aminoethoxydiphenyl borate (2‐APB) and SKF‐96365 reduced mESC proliferation in a concentration‐dependent manner, suggesting that SOCE is important for ESC proliferation. Pluripotent markers, Sox‐2, Klf‐4, and Nanog, were down‐regulated by 2‐APB, suggesting that self‐renewal property of mESCs relies on SOCE. 17β‐estradiol (E2) enhanced mESC proliferation. This enhanced proliferation was associated with an increment of SOCE. Both stimulated proliferation and increased SOCE could be reversed by SOCC blockers suggesting that E2 mediates its stimulatory effect on proliferation via enhancing SOCE. Also, cyclosporin A and INCA‐6, inhibitors of calcineurin [phosphatase that de‐phosphorylates and activates nuclear factor of activated T‐cells (NFAT)], reversed the proliferative effect of E2, indicating that NFAT is involved in E2‐stimulated proliferation. Interestingly, E2 caused the nuclear translocation of NFATc4, and this could be reversed by 2‐APB. These results suggested that NFATc4 is the downstream target of E2‐induced SOCE. The present investigation provides the first line of evidence that SOCE and NFAT are crucial for ESCs to maintain their unique characteristics. In addition, the present investigation also provides novel information on the mechanisms of how E2, an important female sex hormone, affects ESC proliferation. J. Cell. Physiol. 227: 2519–2530, 2012. © 2011 Wiley Periodicals, Inc.
Synthesis of new 3,3′‐(1,4‐phenylene)bis(1,5‐diones) derivatives
Authors: Shao Qiang , Liu Hui , Wang Ting‐Ting , Zeng He‐Ping 
Published: August 2011

AbstractSeveral 3,3′‐(1,4‐phenylene)bis(1,5‐diones) and their chalcone precursors have been prepared in good to excellent yield via aldol addition and Michael addition starting from 3‐acetyl‐2,5‐dimethylfuran or 3‐acetyl‐2,5‐dimethyl‐thiophene with terephthalaldehyde in the presence of appropriate base NaOH or lithium diisopropylamide. The kind and amount of alkali played a critical role in improving the reaction rates and yields of the products. J. Heterocyclic Chem., (2011).
Synthesis and application of silica phenyl sulfonic acid as a solid acid heterogeneous catalyst for one‐pot synthesis of 2‐aryl‐1‐arylmethyl‐1H‐1,3‐benzimidazoles and bis(indolyl)methanes in water
Authors: Veisi Hojat , Sedrpoushan Alireza , Zolfigol Mohammad Ali , Mohanazadeh Farajollah 
Published: August 2011

AbstractSilica phenyl sulfonic acid (SPSA) was prepared and effectively used in the one‐pot synthesis of 2‐aryl‐1‐arylmethyl‐1H‐1,3‐benzimidazoles from o‐phenylenediamine with aldehydes in water in the presence of tetrabutyl ammonium bromide with good to high yield. Also, SPSA was used as a catalyst for the synthesis of bis(indolyl)methanes in water. J. Heterocyclic Chem., (2011).
Value of oxygen‐enhanced MRI of the lungs in patients with pulmonary hypertension: A qualitative and quantitative approach
Authors: Maxien Daniel , Dietrich Olaf , Thieme Sven F. , Förster Stefan , Behr Jürgen , Reiser Maximilian F. , Nikolaou Konstantin 
Published: August 2011

AbstractPurpose:To assess the clinical value of oxygen‐enhanced magnetic resonance imaging (oeMRI) in patients with pulmonary hypertension (PH) by correlation with ventilation/perfusion (V/Q) scintigraphy.Materials and Methods:In all, 33 patients with known PH underwent V/Q scintigraphy and oeMRI. oeMRI was used to assess the regional pulmonary function based on relative‐signal‐enhancement (RSE) and cross‐correlation‐coefficient (CCC) maps, evaluating mean RSE (mRSE), fraction of oxygen‐activated pixels (fOAP), and mean CCC (mCCC). Two reviewers, blinded to the results of scintigraphy, performed visual detection of diseased lung areas.Results:In 26 of the 33 patients (79%) the image quality of oeMRI reached a diagnostic level. In total, 150 lung areas were analyzed and compared. Sensitivities/specificities of oeMRI for detecting these defects were: RSE vs. ventilation scintigraphy 92%/73%; RSE vs. perfusion scintigraphy 60%/87%; CCC vs. ventilation scintigraphy 89%/81%; CCC vs. perfusion scintigraphy 50%/87%. The number of diseased lung areas in oeMRI correlated significantly with the number in V/Q scintigraphy (P < 0.01). mRSE showed a significant correlation with the number of diseased lung areas in ventilation scintigraphy (P < 0.05).Conclusion:oeMRI is feasible in PH patients, yielding an overall moderate agreement between oeMRI and V/Q scans, with a good sensitivity of oeMRI for the detection of ventilation defects as compared with ventilation scintigraphy. J. Magn. Reson. Imaging 2012;35:86‐94. © 2011 Wiley Periodicals, Inc.
Urine testing as a surveillance tool to monitor the impact of HPV immunization programs
Authors: Cuschieri Kate , Nandwani Rak , McGough Pauline , Cook Fiona , Hogg Lesley , Robertson Chris , Cubie Heather 
Published: August 2011

AbstractHPV surveillance is necessary to monitor the impact and success of HPV immunization programs. This study was designed to evaluate the performance of HPV testing in urine to assess its suitability for epidemiological and surveillance purposes. A total of 90 females and 117 males were recruited from a UK drop‐in clinic offering integrated sexual health services. A urine sample and comparator gold‐standard sample (cervical liquid‐based cytology sample or penile swab) was collected from each subject. HPV detection was performed using a PCR‐based assay. Discrepancy between the two overall distributions [urine vs. gold standard (GS)] was measured. At the individual level, sensitivity and specificity of HPV detection in urine versus GS was measured. Prevalence of HPV was higher in urine compared to GS in both females and males. At the individual level, sensitivity of urine versus GS for HPV detection was 90.5% (79.3–96.9) and 55.9% (37.8–72.8) in females and males, respectively. The overall distribution of HPV types in urine was similar to that in gold standard, (P = 0.78; male, P = 0.88; female). Type‐specific matches in urine versus GS were achieved in 71% (61–79.5) and 63.2% (54.2–71.4) of samples from females and males, respectively. Urine, particularly from females, is a useful biospecimen for HPV surveillance purposes. Further examination into the usefulness of urine from males, including choice of relevant gold‐standard comparator, is required. J. Med. Virol. 83:1983–1987, 2011. © 2011 Wiley‐Liss, Inc.
Lymphatic vessel invasion detected by the D2‐40 monoclonal antibody is an independent prognostic factor in node‐negative esophageal squamous cell carcinoma
Authors: Imamura Yu , Watanabe Masayuki , Nagai Yohei , Baba Yoshifumi , Hirashima Kotaro , Karashima Ryuichi , Iwatsuki Masaaki , Yoshida Naoya , Kinoshita Koichi , Kurashige Junji , Iyama Ken‐ichi , Baba Hideo 
Published: August 2011

AbstractBackground and ObjectivesD2‐40 staining has been reported to be useful for both identifying lymphatic vessel invasion (LVI) and counting lymphatic vessel density (LVD) in various cancers. The aim of this study was to clarify the prognostic significance of D2‐40 staining in patients with esophageal squamous cell carcinoma (ESCC).MethodsA total of 159 consecutive patients with ESCC who underwent an esophagectomy with lymph node dissection were eligible. LVI was diagnosed by both hematoxylin–eosin (LVI‐HE) and D2‐40 staining (LVI‐D2‐40) in the largest central sections of the entire tumors, while both the intratumoral and peritumoral LVD were counted by D2‐40 staining. The correlation between the prognosis and clinicopathological factors was investigated.ResultsAn univariate analysis revealed that tumor invasion beyond the muscularis propria, lymph node metastasis (LNM), LVI‐HE, LVI‐D2‐40, high intratumoral LVD, and blood vessel invasion correlated with worse patients' prognosis (P < 0.05). A multivariate analysis revealed LNM to be the only independent prognostic factor in all cases (P = 0.0083). On the other hand, when the prognostic factors of 83 patients without LNM were investigated, LVI‐D2‐40 was revealed to be the only independent prognostic factor (P = 0.048).ConclusionsLVI detected by D2‐40 staining was an independent prognostic factor in patients with node‐negative ESCC. J. Surg. Oncol. 2012; 105:277–283. © 2011 Wiley Periodicals, Inc.
Clinicopathological features and prognostic factors of gastric cancer patients aged 40 years or younger
Authors: Hsieh Feng‐Jen , Wang Yu‐Chao , Hsu Jun‐Te , Liu Keng‐Hao , Yeh Chun‐Nan , Yeh Ta‐Sen , Hwang Tsann‐Long , Jan Yi‐Yin 
Published: August 2011

AbstractBackground and ObjectivesGastric adenocarcinoma (GC) occurs frequently in the sixth decade of life and is uncommon in patients aged 40 years or younger. The aims of this study were to define the clinicopathological features and elucidate the prognostic factors of GC in the young.MethodsBetween 1998 and 2006, 1,815 GC patients undergoing resection were enrolled in a prospective database. The findings for 115 (6.0%) patients aged 40 years or younger were compared with those of 1,009 patients between 56 and 75 years old.ResultsThe group of young patients with GC included significantly more women than the group of old patients (60.0% vs. 37.0%, respectively); young patients also had more T4 lesions (73.9% vs. 61.6%), undifferentiated tumors (85.2% vs. 55.1%), severe desmoplasia (41.4% vs. 12.2%), Lauren's diffuse‐type cancers (55.6% vs. 27.7%), and perineural invasion (69.1% vs. 46.1%). Survival rates in younger patients at 3, 5, and 10 years after resection were 56.8%, 52.0%, and 42.1%, respectively, similar to those in older patients (P = 0.411). Unfavorable independent prognostic factors of GC in the young were degree of nodal involvement (N3 vs. N0; P = 0.001), advanced T status (T3–4 vs. T1–2; P = 0.015), tumor size (>4 vs. ≤4 cm; P = 0.019), and status of resection margins (positive vs. negative; P = 0.044).ConclusionsGC tends to exhibit more aggressive tumor behavior in young patients than in old patients; however, the surgical survival of young and old patients was similar. Advanced nodal involvement (N3) is the most important independent prognostic factor in the young. J. Surg. Oncol. 2012; 105:304–309. © 2011 Wiley Periodicals, Inc.
Synthesis and application of clindamycin succinate as a novel chiral selector for capillary electrophoresis
Authors: Wu Jianfeng , Liu Peng , Wang Li , Tian Hailin , Wang Qingwei , Zhang Shengyong 
Published: August 2011

AbstractA novel chiral selector, clindamycin succinate, was synthesized and first used as a chiral selector in capillary electrophoresis (CE). The chiral resolution ability of this kind of clindamycin derivation was studied by CE using some racemic drugs as model analytes. From the experimental results, it was found that both resolution and selectivity of the selector were dependent on the following parameters: concentration of chiral selectors, pH of the running buffer, temperature of the capillary column, applied voltage and organic modifier used. The results show that the chiral selector possesses high resolution toward some racemic drugs, including ofloxacin, chlorphenamine, tryptophan, propranolol, sotalol and metoprolol. Excellent chiral resolution of these tested drugs was achieved under the optimal conditions of 50 mM clindamycin succinate, 10% MeOH v/v, 50 mM Tris buffer, pH 4.0, at 22 kV and 20°C within 25 min.