bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic Registration of Preablation Imaging With a Catheter Geometry to Guide Ablation in a Swine Model: Validation of Image Integration and Assessment of Catheter Navigation Accuracy
Authors: WEST J. JASON , PATEL AMIT R. , KRAMER CHRISTOPHER M. , HELMS ADAM S. , OLSON ERIC S. , RANGAVAJHALA VAMSEE , FERGUSON JOHN D. 
Published: August 2009

Image Integration with a Catheter Mapping System. Background: Catheter ablation of atrial and ventricular tachyarrhythmia involves anatomically based cardiac ablation strategies. CT and MRI images provide the most detailed cardiac anatomy available. Integration of these images into a mapping system should produce detailed and accurate models suitable to guide ablation.
Objective:
The purpose of this study was to validate and assess the accuracy of a novel CT and MRI image integration algorithm designed to facilitate catheter navigation and ablation.
Methods:
Using a lateral thoracotomy, markers were sutured to the epicardial surface of each cardiac chamber in 12 swine. Detailed CT/MRI anatomy was imported into the mapping system. The CT/MRI image was then integrated with a detailed catheter geometry of the relevant chamber using a new image integration algorithm. The epicardial markers, identified from the CT/MRI images, were then displayed on the surface of the integrated image. Guided only by the integrated CT/MRI, a single RF lesion was directed at the corresponding endocardial site for each epicardial marker. At autopsy, the distance from the endocardial RF lesion to the target site was assessed.
Results:
The mean position error (CT/MRI) for the left atrium was 2.5 ± 2.4 mm/5.1 ± 3.9 mm, for the right atrium 6.2 ± 6.5 mm/4.3 ± 2.2 mm, for the right ventricle 6.2 ± 4.3 mm/6.6 ± 5.3 mm, and for the left ventricle 4.7 ± 3.4 mm/3.1 ± 2.7 mm. There was no cardiac perforation or tamponade.
Conclusion:
CT and MRI images can be effectively utilized for catheter navigation when integrated into a mapping system. This novel registration module with dynamic registration provides effective guidance for ablation. (J Cardiovasc Electrophysiol, Vol. 21, pp. 81–87, January 2010)
Presence of Aggregatibacter actinomycetemcomitans in young individuals: a 16‐year clinical and microbiological follow‐up study
Authors: Höglund Åberg Carola , Sjödin Bengt , Lakio Laura , Pussinen Pirkko J. , Johansson Anders , Claesson Rolf 
Published: August 2009

AbstractAim: To look for clinical signs of periodontal disease in young adults who exhibited radiographic bone loss and detectable numbers of Aggregatibacter actinomycetemcomitans in their primary dentition.Material and methods: Periodontal status and radiographic bone loss were examined in each of the subjects 16 years after the baseline observations. Techniques for anaerobic and selective culture, and checkerboard, were used to detect periodontitis‐associated bacterial species. The isolated A. actinomycetemcomitans strains were characterized by polymerase chain reaction.Results: Signs of localized attachment loss were found in three out of the 13 examined subjects. A. actinomycetemcomitans was recovered from six of these subjects and two of these samples were from sites with deepened probing depths and attachment loss. Among the isolated A. actinomycetemcomitans strains, serotypes a–c and e, but not d or f, were found. None of the isolated strains belonged to the highly leucotoxic JP2 clone, and one strain lacked genes for the cytolethal distending toxin.Conclusions: This study indicates that the presence of A. actinomycetemcomitans and early bone loss in the primary dentition does not necessarily predispose the individual to periodontal attachment loss in the permanent dentition.
The temporal course of mucoperiosteal flap revascularization at guided bone regeneration‐treated implant sites: a pilot study
Authors: Milstein Dan M. J. , Mathura Keshen R. , Lindeboom Jérôme A. H. , Ramsoekh Dewkoemar , Lindeboom Robert , Ince Can 
Published: August 2009

AbstractAims: To investigate post‐operative capillary density regeneration in healing mucoperiosteal flaps at guided bone regeneration‐treated implant sites.Material and Methods: A non‐invasive post‐operative investigation was performed in 10 patients using orthogonal polarization spectral (OPS) imaging for assessment of capillary density during the course of mucoperiosteal flap wound healing for 6 weeks in patients receiving dental implants.Results: The greatest increase in capillary regeneration occurred in the early wound‐healing phase, during weeks 1 and 2, and recovery to baseline was achieved between weeks 4 and 5. A comparison of adjacent OPS measurements indicated that differences between the time point immediately following administration of local anaesthesia and directly post‐operatively ( p=0.002), between a directly post‐operative time point and after 1 week (p=0.009), and between post‐operative weeks 1 and 2 (p=0.036) were statistically significant.Conclusions: The early healing phase of mucoperiosteal flaps is characterized by rapid capillary regeneration. OPS imaging enabled the possibility to monitor and quantify the temporal development of mucoperiosteal flap revascularization following periodontal surgery.
Contribution of skin biometrology to the diagnosis of the Ehlers–Danlos syndrome in a prospective series of 41 patients
Authors: Catala‐Pétavy C. , Machet L. , Georgesco G. , Pétavy F. , Maruani A. , Vaillant L. 
Published: August 2009

Background/purpose: The diagnosis of the Ehlers–Danlos syndrome (EDS) is primarily clinical. Clinical signs result from modifications of the rheological properties of the skin: thickness, extensibility and hydration. Our main objective was to demonstrate what skin biometry can contribute to the diagnosis and evaluation of the different types of EDS.Methods: Forty‐one patients clinically diagnosed with EDS were paired by age and sex to 41 healthy subjects with no known dermatologic disease, in particular connective tissue diseases. We measured skin thickness, extensibility, hydration and sebum secretion by skin ultrasonography, use of a Cutometer®, a Corneometer® and a Sebumeter®.Results: The skin is thinner in all three disease types (classical, hypermobility and vascular). It is more extensible only in the classical type, and more hydrated and fatty in the hypermobility type.Conclusion: Non‐invasive measurement of skin thickness and elasticity are quantitative parameters that can be used to better diagnose EDS. Moreover, the sebaceous rate can help to diagnose the hypermobility type whereas the immediate retraction distinguishes the classical form from vascular and hypermobile types.
Measuring human skin buffering capacity: an in vitro model
Authors: Zhai Hongbo , Chan Heidi P. , Farahmand Sara , Maibach Howard I. 
Published: August 2009

Background/purpose: It has been thought that skin possesses buffering capacity. This study measured the skin buffering capacity against two model solutions of acid and base at three concentrations with an in vitro system.Methods: Ten microliters of model base (sodium hydroxide – NaOH) and acid (hydrochloric acid – HCl) solutions at concentrations of 0.025, 0.05, and 0.1 N was applied to human cadaver skin (3.18 μL/cm2) placed onto glass diffusion cells. Phosphate‐buffered saline (PBS) was used as a standard buffer solution. Deionized water served as the negative control, whereas untreated skin served as the blank control. Skin pH was read and recorded immediately following dosing (0 time), and at 10 and 30 min of post‐dosing. After the 30 min of dosing, each skin, except untreated skin (blank control), was then washed by applying 1 cm3 of deionized water. The pH on each washed skin was measured immediately following washing, and the pH measurement was repeated at 10 and 30 min of post‐washing. Six replicates were conducted.Results: The pH values sharply significantly increased (P<0.05) immediately following dosing with NaOH at all concentrations (the highest concentration, caused the highest pH), and then decreased closely to baselines within 30 min post‐application but still remained at significantly (P<0.05) higher values when compared with the blank control (untreated skin). HCl (acid) significantly (P<0.05) decreased skin pH immediately following dosing with all concentrations (the highest concentration, caused the lowest pH) and then restored rapidly to baseline. There was no significant difference in post‐washing procedures on the skins that were pre‐treated with the acid (HCI) solutions. However, with all base solutions (NaOH) pre‐treated skin, pH values were significantly higher (P<0.05) at all time points post‐washing. Furthermore, both PBS and water controls significantly elevated (P<0.05) the pH values following washing.Conclusion: Skin pH and its buffering capacity can be measured on human cadaver skin in vitro, which may partially replicate the response of in vivo skin. Dose–response was noted; i.e. the higher concentration caused larger changes in skin pH. In addition, the restoration of skin pH is relatively faster with acid when compared with base treatment. Clinical implications are offered.
Effects of 5′‐uridylic acid feeding on postprandial plasma concentrations of metabolites and metabolic hormones in pre‐weaning goats
Authors: OHTANI Yoshihisa , KOBAYASHI Yosuke , TAKAHASHI Tatsuyuki , HAGA Satoshi , TAKAGI Mutsuo , MASHIKO Takanori , KATOH Kazuo , OBARA Yoshiaki 
Published: August 2009

ABSTRACT5′‐Uridylic acid (UMP), which is present at high concentrations in cow's colostrum, has been shown to cause a reduction in increased plasma levels of insulin and glucose after ingestion of milk replacer in pre‐weaning calves. However, the precise mechanisms of UMP action have not been investigated, and its action has not been investigated in other pre‐weaning ruminants. In order to demonstrate whether UMP causes changes in postprandial metabolic and hormonal parameters in pre‐weaning goats, 11 Saanen kids were given milk replacer (twice a day) without (n = 5) or with (n = 6) UMP (1 g for each meal, 2 g/day for each head) for 14 days. Analysis of blood samples taken in the morning of day 14 demonstrated that the feeding of milk replacer with UMP abolished the significant changes in postprandial plasma glucose, NEFA, GH and insulin concentrations induced by feeding of milk replacer alone, and demonstrated a tendency to increase IGF‐I levels. However, there was no significant difference between the two groups at any sampling time. We conclude that UMP feeding with milk replacer showed a tendency to blunt the postprandial changes in levels of some plasma metabolites and hormones that are induced by replacer alone in pre‐weaning goats.
Extracellular signal‐regulated kinase (ERK) activation in chicken heterophils stimulated with phorbol 12‐myristate 13‐acetate (PMA), Formyl‐methionylleucyl‐phenylalanine (fMLP) and lipopolysaccharide (LPS)
Authors: GERILECHAOGETU  , HANIEH Hamza , ABE Asaki , KONDO Yasuhiro 
Published: August 2009

ABSTRACTThe signaling pathways leading to the activation of extracellular signal‐regulated kinase (ERK) by phorbol 12‐myristate 13‐acetate (PMA), formyl‐methionylleucyl‐phenylalanine (fMLP) and lipopolysaccharide (LPS) in chicken heterophils were examined. To determine the mechanism of ERK's activation and its relation with the influx of calcium ions, heterophils were stimulated by PMA, fMLP and LPS. ERK was not activated by fMLP. LPS‐ and PMA‐stimulated activation of ERK, based on Western blotting with antibodies against the phosphorylated form of ERK, was attenuated by the pretreatment of cells with the intracellular calcium chelator BAPTA/AM (1,2‐bis (o‐aminophenoxy) ethane‐N,N,N′,N′‐tetraacetic acid) but not with the extracellular calcium chelator EGTA (glycol‐bis(2‐aminoethylether)‐N,N,N′,N′‐tetraacetic acid). Exposure of cells to the protein kinase C (PKC) inhibitor GF109203X inhibited the LPS‐ and PMA‐stimulated phosphorylation of ERK in a concentration‐dependent manner. The LPS‐stimulated phosphorylation was inhibited by pretreatment with the phospholipase C (PLC) inhibitor U73122 but not the phosphatidylinositol 3‐kinase (PI3K) inhibitor LY294002. These results indicate that the LPS‐induced phosphorylation of ERK in the chicken heterophils is mediated by PLC, PKC and intracellular calcium, and the PMA‐stimulated phosphorylation is dependent on intracellular calcium ion and PKC.
Phosphodiesterase Type 5 Inhibitors Facilitate Noncontact Erections in Male Rats: Site of Action in the Brain and Mechanism of Action
Authors: Sanna Fabrizio , Succu Salvatora , Boi Antonio , Melis Maria Rosaria , Argiolas Antonio 
Published: August 2009

ABSTRACTIntroductionOrally active phosphodiesterase type 5 inhibitors (PDE5i), used in the treatment of erectile dysfunction, facilitate the relaxation of cavernous smooth muscle tissues by reducing the degradation of cyclic guanosine monophosphate.AimsThe aims of this article were to determine whether PDE5i facilitate penile erection and male sexual behavior by acting also on the central nervous system and to investigate their mechanism of action at central level.MethodsPDE5i (sildenafil, vardenafil, and tadalafil) given intraperitoneally (i.p.) (5 mg/kg and 10 mg/kg), intracerebroventricularly (i.c.v.) (10 µg and 50 µg), or into the ventral tegmental area (VTA) (10 µg) were tested in the noncontact erection test in male Sprague-Dawley rats screened for their ability to display or not display this sexual response. Extracellular dopamine was measured in the dialysate obtained from the nucleus accumbens by intracerebral microdialysis on injection of PDE5i into the VTA.Main Outcome MeasuresNoncontact erections were counted after intraperitoneal, intracerebroventricular, or intra-VTA treatment with PDE5i. Extracellular dopamine was measured in the dialysate from the nucleus accumbens when sildenafil or vardenafil was given into the VTA.ResultsPDE5i induced a significant increase of noncontact erections in male rats displaying this sexual response following intraperitoneal or intracerebroventricular administration at the highest dose tested. However, both doses significantly increased noncontact erections in male rats not displaying this sexual response. Similar results were found when PDE5i were injected into the caudal VTA. Noncontact erections increased concomitantly to a rise in extracellular dopamine in the dialysate from the nucleus accumbens.ConclusionsThe results suggest that PDE5i may increase sexual arousal by acting in the central nervous system. This effect may be mediated (at least in part) by the activation of mesolimbic dopaminergic neurons.
Sexual Function in Male Patients Undergoing Treatment for Renal Failure: A Prospective View
Authors: Nassir Anmar 
Published: August 2009

ABSTRACT

Introduction
Chronic renal failure in males causes wide-ranging disturbances including sexual dysfunction. The percentage and progression of sexual dysfunction in patients entering a dialysis program require further evaluation.

Aim
Our aim was to determine the ongoing effect of standard renal failure treatment on sexual function.

Methods
The sexual function was assessed prospectively, upon initiation of dialysis and every 10–12 months while on dialysis or after kidney transplantation. Participants were adult males with sexual partners.

Main Outcome Measure
The semiquantitative standard International Index of Erectile Function questionnaire was used initially as a baseline and compared with all subsequent follow-up measures.

Results
Fifty-two patients fulfilled the eligibility criteria and completed the questionnaire. Of the 52 subjects, 25 were on hemodialysis and 27 were on peritoneal dialysis. Only 17.3% of participants were potent upon entry into the study. Of the rest, 71% was classified as suffering from severe erectile dysfunction (ED). Sexual desire appeared less affected when compared with other domains. Of the study participants, 67% expressed an interest in treatment for ED, but only 12% had ever received any such therapy. Follow-up ranged from 10 months to 48 months. After excluding kidney-transplanted patients, ED scores on entry and at four subsequent reassessments were almost identical and showed no significant statistical differences. Patients showed significant improvement in ED score after kidney transplantation, with scores remaining high for 20–36 months of follow-up, compared with pre-transplantation.

Conclusions
This prospective study suggests that dialysis does not benefit sexual function, although a benefit was seen in a subset of men undergoing renal transplantation. We conclude that sexual function in men beginning dialysis should be assessed, and treatment for ED should be offered if appropriate.
ORIGINAL RESEARCH—PAIN: Sexual Functioning in Women with Chronic Pelvic Pain: The Role of Anxiety and Depression
Authors: ter Kuile Moniek M. , Weijenborg Philomeen T.M. , Spinhoven Philip 
Published: August 2009

ABSTRACTIntroductionChronic pelvic pain (CPP) in women is a long lasting and often disabling condition. It seems reasonable to expect that as a result of the pain, extreme fatigue and/or emotional problems, women with CPP may report a variety of sexual problems.AimThe present study investigated differences in the report of sexual problems in women with CPP compared with healthy controls, and whether the association of CPP with sexual problems was moderated or mediated by somatic and psychological factors as manifested in women suffering from CPP.MethodOne hundred fifty-four women with CPP and 58 age-matched controls completed self-report measures for sexual functioning, pain, physical impairment, anxiety, depression, and sexual and physical abuse.Main Outcome MeasureGolombok Rust Inventory of Sexual Satisfaction.ResultsWomen with CPP reported higher levels of vaginistic complaints, sexual avoidance, nonsensuality and sexual dissatisfaction than healthy controls. Sexual problems were associated with anxiety, depression, and sexual abuse history but not with somatic factors as pain and physical impairment. Anxiety as well as depression, irrespective of the report of sexual abuse experiences, mediated the effect of CPP on sexual problems. Sexual abuse was a general predictor of sexual problems in both women with CPP and controls.ConclusionsAnxiety and depression constitute important factors in the evaluation of sexual problems in women with CPP.
The Relevance of Sexual Responsiveness to Sexual Function in Male Stroke Patients
Authors: Duits Annelien , Van Oirschot Nicole , Van Oostenbrugge Robert Jan , Van Lankveld Jacques 
Published: August 2009

ABSTRACT

Introduction
Stroke may have negative consequences for the patients’ quality of life, including sexual function. Whereas physical impairment will influence sexual positions and movement during sex, depression and medication may reduce sexual desire. So far, data on sexual dysfunction after stroke are scant. Although some support for physical as well as psychological explanations has been shown, further research to find the remedies for those patients with sexual problems after stroke is needed. The focus of the present study is on the identification of relevant psychological factors.

Aim
The aim of this study was to study the impact of anxiety, depression, and sexual responsiveness on sexual function in male stroke patients.

Methods
Nineteen male stroke patients completed a number of self-report measures to assess psychological and sexual factors.

Main Outcome Measures
Sexual function based on the International Index of Erectile Function, anxiety and depression based on the Symptom Checklist-90, and sexual responsiveness based on the Sexual Inhibition/Sexual Excitation Scale, including propensities for sexual excitation and sexual inhibition as a result of both performance failure and performance consequences, were assessed.

Results
Sexual excitation was positively related to sexual desire, whereas inhibition because of the threat of performance failure was negatively related to orgasmic function and sexual desire (P &lt; 0.01). Patients with high levels of inhibition because of threat of performance failure were more likely to report low scores on overall sexual function than those with low levels.

Conclusions
Although the statistical power is rather low, the results show the relevance of sexual responsiveness to sexual function in male stroke patients. The present study can be considered as a first step toward building a theoretical framework of relevant psychological and physical factors, which is needed to develop adequate interventions for those patients with sexual problems after stroke.
Effects of Dialysis Purity on Uremic Dyslipidemia
Authors: Schiffl Helmut , Lang Susanne M 
Published: August 2009

AbstractDyslipidemia, a prominent feature of end‐stage renal disease, is considered a risk factor for premature atherosclerosis in hemodialysis (HD) patients. Dyslipidemia is related to loss of kidney function as well as use of low‐flux cellulosic dialyzer membranes, but the effects of dialysate purity are unknown. Forty‐eight incident HD patients started high‐flux polysulfone maintenance HD, either with conventional (potentially contaminated) or with on‐line produced ultrapure dialysate. The quality of the dialysis fluid (CFU/mL, endotoxin concentration), markers of inflammation (C‐reactive protein, Il‐6), and parameters of the lipid profile and oxidative stress (oxidized low‐density lipoprotein) were measured before initiation of HD, and after 6, 12 and 24 months on HD. Compared to baseline, treatment with conventional (mildly contaminated) dialysate significantly increased the uremic low‐grade systemic inflammatory response syndrome (SIRS), augmented uremic dyslipidemia (triglycerides by +21%, and high‐density lipoprotein (HDL) cholesterol by −10%) and enhanced oxidative stress. In contrast, the use of ultrapure dialysate significantly decreased uremia‐associated SIRS, dyslipidemia (triglycerides −7% and HDL cholesterol +11%) and oxidative stress. Ultrapure dialysis fluid improves potential parameters of cardiovascular risk by decreasing inflammatory reactions, improving uremic dyslipidemia and lowering oxidative stress.