bluuebunny/crossref_metadata_2025_split huggingface dataset:
Methylmalonic acidemia triggers lysosomal-autophagy dysfunctions
Authors: Costanzo Michele , Cevenini Armando , Kollipara Laxmikanth , Caterino Marianna , Bianco Sabrina , Pirozzi Francesca , Scerra Gianluca , D’Agostino Massimo , Pavone Luigi Michele , Sickmann Albert , Ruoppolo Margherita 
Published: May 2024

Abstract
Background
Methylmalonic acidemia (MMA) is a rare inborn error of propionate metabolism caused by deficiency of the mitochondrial methylmalonyl-CoA mutase (MUT) enzyme. As matter of fact, MMA patients manifest impairment of the primary metabolic network with profound damages that involve several cell components, many of which have not been discovered yet. We employed cellular models and patients-derived fibroblasts to refine and uncover new pathologic mechanisms connected with MUT deficiency through the combination of multi-proteomics and bioinformatics approaches.

Results
Our data show that MUT deficiency is connected with profound proteome dysregulations, revealing molecular actors involved in lysosome and autophagy functioning. To elucidate the effects of defective MUT on lysosomal and autophagy regulation, we analyzed the morphology and functionality of MMA-lysosomes that showed deep alterations, thus corroborating omics data. Lysosomes of MMA cells present as enlarged vacuoles with low degradative capabilities. Notwithstanding, treatment with an anti-propionigenic drug is capable of totally rescuing lysosomal morphology and functional activity in MUT-deficient cells. These results indicate a strict connection between MUT deficiency and lysosomal-autophagy dysfunction, providing promising therapeutic perspectives for MMA.

Conclusions
Defective homeostatic mechanisms in the regulation of autophagy and lysosome functions have been demonstrated in MUT-deficient cells. Our data prove that MMA triggers such dysfunctions impacting on autophagosome-lysosome fusion and lysosomal activity.
Seroepidemiological survey of contagious bovine pleuropneumonia among cattle in El Jazeera State (Central Sudan)
Authors: Hussien Mohammed , Abdelhabib Eslah , Hamid Abdalsalam , Musa Azza , Fadolelgaleel Huyam , Alfaki Shima , El Hussein Abdel Rahim 
Published: May 2024

Abstract
Background
Contagious bovine pleuropneumonia (CBPP) is an economically important infectious disease that is characterized by a variable course and insidious nature. A cross-sectional study was conducted in El Jazeera State, Central Sudan, to determine the seroprevalence and risk factors of CBPP in cattle from seven localities. A total of 218 serum samples were randomly collected from apparently healthy cattle aged older than 6 months between April and May 2021 and were tested serologically using a commercial ELISA kit.

Results
The overall seroprevalence of CBPP was 50.5% (110/218). Univariate analysis showed a significant difference (p < 0.05) between sex, locality and water source and seropositivity to CBPP. Multivariate analysis revealed that the independent risk factors (sex, locality and water source) were also statistically significant (p < 0.05). At herd level, out of 20 herds 16 (80%) proved to be positive for CBPP antibodies. It is apparent from the present study that CBPP infection is prevalent among cattle in El Jazeera State, Central Sudan.

Conclusions
To the best of our knowledge, this is the first seroepidemiological study on CBPP infection in Central Sudan. The authors recommend major awareness both in the production area and quarantine centers, as CBPP may result in restrictions on the international trade of animals and animal products.
Patterns of alteration in boar semen quality from 9 to 37 months old and improvement by protocatechuic acid
Authors: Hu Ruizhi , Yang Xizi , Gong Jiatai , Lv Jing , Yuan Xupeng , Shi Mingkun , Fu Chenxing , Tan Bie , Fan Zhiyong , Chen Liang , Zhang Hongfu , He Jianhua , Wu Shusong 
Published: May 2024

Abstract
Background
Comprehending the patterns of alteration in boar semen quality and identifying effective nutritional interventions are crucial for enhancing the productivity of commercial pig systems. This study aimed to examine the alteration in semen quality in boars, and assess the impact of protocatechuic acid (PCA) on semen quality during the phase of declining semen quality.

Methods
In Exp. 1, a total of 38 Pig Improvement Company (PIC) boars were selected and their semen quality data were recorded from the age of 9 to 37 months. In Exp. 2, 18 PIC boars (28 months old) were randomly assigned into three groups (n = 6) and fed a basal diet, a basal diet containing 500 or 1,000 mg/kg PCA, respectively. The experiment lasted for 12 weeks.

Results
The semen volume, concentration, and total number of spermatozoa in boars exhibited an increase from 9 to 19 months old and showed a significant linear decreased trend in 28, 24, and 22 months old. Sperm motility displayed an upward trajectory, reaching its peak at 20 months of age, and showed a significant linear decreased trend at 20 months old. Dietary supplementation of PCA demonstrated an effect to mitigate the decrease in semen volume, concentration of spermatozoa, total number of spermatozoa (P > 0.05), and significantly increased the sperm motility (P < 0.05). Moreover, supplementation of 1,000 mg/kg PCA significantly increased the sperm viability (P < 0.05). Analysis on cellular signaling pathways revealed that PCA restored serum testosterone levels and alleviated oxidative damage by upregulating the expression of HO-1, SOD2, and NQO1 in testicular stromal cells. Notably, PCA can enhance phosphorylation by selectively binding to AMP-activated protein kinase (AMPK) protein, thereby improving sperm mitochondrial function and augmenting sperm motility via PGC-1/Nrf1.

Conclusions
These data elucidated the pattern of semen quality variation in boars within the age range of 9 to 37 months old, and PCA has the potential to be a natural antioxidant to enhance sperm quality through modulation of the AMPK/PGC-1/Nrf1 signaling pathway.
Combination of percutaneous thermal ablation and adoptive Th9 cell transfer therapy against non-small cell lung cancer
Authors: Pan Hanbo , Tian Yu , Pei Siyu , Yang Wanlin , Zhang Yanyang , Gu Zenan , Zhu Hongda , Zou Ningyuan , Zhang Jiaqi , Jiang Long , Hu Yingjie , Shen Shengping , Wang Kai , Jin Haizhen , Li Ziming , Zhang Yanyun , Xiao Yichuan , Luo Qingquan , Wang Hui , Huang Jia 
Published: May 2024

Abstract
Background
Non-small cell lung cancer (NSCLC) is one of the predominant malignancies globally. Percutaneous thermal ablation (PTA) has gained widespread use among NSCLC patients, with the potential to elicit immune responses but limited therapeutic efficacies for advanced-stage disease. T-helper type 9 (Th9) cells are a subset of CD4+ effector T cells with robust and persistent anti-tumor effects. This study proposes to develop PTA-Th9 cell integrated therapy as a potential strategy for NSCLC treatment.

Methods
The therapeutic efficacies were measured in mice models with subcutaneously transplanted, recurrence, or lung metastatic tumors. The tumor microenvironments (TMEs) were evaluated by flow cytometry. The cytokine levels were assessed by ELISA. The signaling molecules were determined by quantitative PCR and Western blotting. The translational potential was tested in the humanized NSCLC patient-derived xenograft (PDX) model.

Results
We find that PTA combined with adoptive Th9 cell transfer therapy substantially suppresses tumor growth, recurrence, and lung metastasis, ultimately extending the survival of mice with NSCLC grafts, outperforming both PTA and Th9 cell transfer monotherapy. Analysis of TMEs indicates that combinatorial therapy significantly augments tumor-infiltrating Th9 cells, boosts anti-tumor effects of CD8+ T cells, and remodels tumor immunosuppressive microenvironments. Moreover, combinatorial therapy significantly strengthens the regional and circulation immune response of CD8+ T cells in mice with tumor lung metastasis and induces peripheral CD8+ T effector memory cells in mice with tumor recurrence. Mechanically, PTA reinforces the anti-tumor ability of Th9 cells primarily through upregulating interleukin (IL)-1β and subsequently activating the downstream STAT1/IRF1 pathway, which could be effectively blocked by intercepting IL-1β signaling. Finally, the enhanced therapeutic effect of combinatorial therapy is validated in humanized NSCLC PDX models.

Conclusions
Collectively, this study demonstrates that combinatorial therapy displays robust and durable anti-tumor efficacy and excellent translational potential, offering excellent prospects for translation and emerging as a promising approach for NSCLC treatment.
Isolation and expression analysis of cellulose synthase 3 (Ces3) genes from sugarcane (Saccharum officinarum L.)
Authors: Huang Cheng-Mei , Wu Kai-Chao , Verma Krishan K. , Luo Hai-Bin , Deng Zhi-Nian , Shi Guo-Ying , Cao Hui-Qing , Wu Xing-Jian , Ye Li-Ping , Wei Yuan-Wen , Xu Lin 
Published: May 2024

AbstractPlant cellulose synthase is one of the important glycosyltransferases, which catalyzes the synthesis of the paracrystalline of H-bonded-β-(1,4)-glucose chains. This study isolated the cellulose synthase 3 (Ces3) sequence from sugarcane (Saccharum officinarum L.) leaves. SoCes3 (GenBank accession No. MG324347) has a full-length cDNA sequence of 3625 bp. It contains an open reading frame (3225 bp), encoding 1074 amino acids with a molecular weight of about 120.89 kDa and isoelectric point of 6.26. SoCes3 protein showed high activity with other plant cellulose synthases. The recombinant protein contains plant cellulose synthase (Ces) protein conservative domains. In subcellular localization experiments, the fusion protein of SoCes3 with green fluorescent protein (GFP) was specifically localized in the cell membrane. The gene expression of SoCes3 was found in the leaf, leaf sheath, and internodes of the sugarcane stem. The highest expression level was found in the internode, especially with the highest expression level in the 5th internode and lowest in the leaves, and the gene expression level of SoCes3 was upregulated by PP333 and not in gibberellic acid-treated plants. It was conducted in tobacco plants to understand the biotechnological potential of SoCes3. The contents of cellulose and lignin were increased in SoCes3-overexpressing tobacco. Transcriptomic analysis showed that the transgenic tobacco induced different genes associated with different biological regulatory processes. Differentially expressed genes (DEGs) mediated plant hormone signal transduction, starch and sucrose metabolism signaling pathways were widely induced and mostly upregulated. The transcription levels in SoCes3-overexpressing transgenic lines were higher than wild-type.
Graphical Abstract
Formative research to adapt a cardiac rehabilitation program to breast cancer survivors: the heart health after cancer treatment (HEART-ACT) study
Authors: Chang Alison , Boyd Alisa , Leung Ivan , Trejo Evelin , Dixit Niharika , Mallidi Jaya , Win Sithu , Beatty Alexis L. 
Published: May 2024

Abstract
Background
Breast cancer survivors are disproportionately at risk for cardiovascular disease; exercise-based interventions may improve cardiovascular health. The objective of this formative research is to better understand the needs of patients and barriers to participation in an adapted cardiac rehabilitation program for diverse breast cancer survivors in an urban safety net setting.

Methods
We recruited 30 participants (10 English-speaking, 10 Spanish-speaking, and 10 Cantonese-speaking) who had received treatment with curative intent for breast cancer from an urban safety net hospital between November 9, 2021, to August 30, 2022. Participants completed surveys and interviews about perspectives on health behaviors and participating in an adapted cardiac rehabilitation program. Interviews were qualitatively analyzed using rapid template analysis with pre-selected constructs from the Theory of Planned Behavior, Unified Theory of Acceptance and Use of Technology, and Consolidated Framework for Implementation Research, as well as emergent codes. We developed a Participant User Journey for a program based on responses and conducted human-centered design sessions with 8 participants to iteratively revise the Participant User Journey.

Results
Among 30 participants, mean age was 56.7 years (standard deviation [SD] 10.2) with 100% female sex assigned at birth; 1 participant withdrew before completing study procedures. Most participants had limited health literacy (18/29, 62%). Mean body mass index was 31.4 (SD 8.3), 21/29 (72%) had blood pressure below 140/90 mmHg, and 12/29 (41%) had blood pressure below 130/80. Mean 6-minute walk distance was 384.9 meters (SD 78.3). The desired benefits of a program included healthy living and prevention of cancer recurrence. Barriers to participation included motivation, social support, transportation, and concerns about exercise safety. Participants emphasized the need for practicality, such as fitting physical activity into daily life and nutrition support, including recipes and shopping lists. Trusted experts and cultural and language concordance were viewed as important aspects of the program.

Conclusions
Through participant interviews and human-centered design sessions, we developed the HEART-ACT program, a 12-week multi-disciplinary program addressing physical activity, nutrition, emotional well-being, cardiovascular risk, survivorship, and other components if indicated (e.g., tobacco cessation). Future research will test the effects of this program on patient-centered outcomes.
The fundamental issues in promoting modern civilization of the Chinese Nation
Authors: Wang Yanzhong 
Published: May 2024

AbstractSince China entered the Xi Jinping Era, the CPC has formally put forward a series of significant theories on China’s future development path, including Xi Jinping Thought on Culture. These theories have sparked active discussions and responses within both domestic and international academic and theoretical circles. This paper argues that when analyzing the major relationships between Chinese civilization and other civilizations worldwide, the Chinese path to modernization and the modern civilization of the Chinese Nation, and Chinese culture and its constituent ethnic cultures, we should further elucidate relationships and adhere to the three foundational principles: subjectivity, the contemporaneity of Chinese culture, and the arterial and leading attributes of Chinese culture, which encapsulate the core issues in fostering the modern civilization of Chinese Nation. A proper understanding and nuanced handling of these issues not only bolsters the perpetuation and evolution of China’s excellent traditional culture but also propels the trajectory of the Chinese path to modernization and the overarching construction of the modern civilization of Chinese Nation.
Physical activity, sedentary behaviour, and sleep knowledge and self-efficacy among parents of young children in Canada
Authors: Bruijns Brianne A. , Bourke Matthew , Saravanamuttoo Kendall , Tucker Patricia 
Published: May 2024

Abstract
Background
Engaging in healthy movement behaviours in early childhood is beneficial for children’s development, and parents play a critical role in shaping movement habits during these formative years. This study aimed to explore parents’ knowledge of the Canadian 24-Hour Movement Guidelines for the Early Years (The Guidelines) and their perceived knowledge and self-efficacy of movement behaviour concepts in early childhood. The influence of sociodemographic characteristics on these variables was also examined.

Methods
Via a cross-sectional online survey with parents/guardians (n = 576) of young children in Canada, participants’ knowledge of The Guidelines (11 items), and perceived knowledge (11 items; 5-point scale) and self-efficacy (11 items; 11-point scale) of movement behaviour concepts in early childhood were examined. Descriptive statistics were calculated for independent variables and multivariate regression models were estimated to examine sociodemographic factors that were associated with participants’ knowledge and self-efficacy.

Results
Few participants (11.9%) reported to be familiar with The Guidelines. Parents were most knowledgeable about the screen time guideline for children under 2 years (75.0% correctly identified the guideline) and least familiar with the recommendation for toddler/preschooler total physical activity (14.7% correctly identified the guideline). Parents/guardians perceived they were the most knowledgeable about safe sleep practices (65.9% very/extremely knowledgeable) and least knowledgeable about muscle- and bone-strengthening activities (71.8% not at all/somewhat knowledgeable). Overall, parents/guardians were moderately confident in promoting healthy movement behaviours (M = 6.01; SD = 1.73). Participants with higher levels of education reported significantly higher perceived knowledge compared to those who were less educated (p = .004), and parents/guardians identifying as White reported significantly higher levels of self-efficacy compared to parents/guardians from minority ethnic groups (p = .028). Finally, parents/guardians who were more physically active reported both higher perceived knowledge (p < .001) and self-efficacy (p < .001) than those who were less active.

Conclusion
These findings shed light on the need to raise awareness of The Guidelines among parents of young children and highlight specific movement behaviour concepts in need of focus in future training for this population. Targeted education for parents/guardians from lower socioeconomic groups is also needed to address equity concerns.
Implication of fast shear wave azimuth and critically stressed fractures in a coalbed methane reservoir
Authors: Banerjee Abir , Bhattacharyya Gautam , Lugun Nawin , Das Aloke , Johri Aditya 
Published: May 2024

The orientation of the fractures and stresses within coal seams plays a critical role in gas production from coalbed methane reservoirs. In this study, we used resistivity images and sonic logs to investigate these parameters, aiming to (1) establish the relationship between fracture orientations and the polarization angles of fast shear waves and (2) detect active fractures in the coal seam. Shear waves in anisotropic formations are split into fast and slow components, with Alford’s rotation method used to determine the polarization angles of the fast shear wave. We found that the fast shear wave aligns with the direction of higher fracture intensity. Subsequently, we incorporated the poroelastic strain model to estimate the vertical ([Formula: see text]), maximum horizontal ([Formula: see text]), and minimum horizontal ([Formula: see text]) stresses in the wellbore. These stress magnitudes aided in identifying the faulting regime and were corroborated by vertical opening mode fractures. Validation of [Formula: see text] and [Formula: see text] involved comparisons with the breakout width in image logs and the closure pressure observed during hydraulic fracturing treatments. Applying Mohr’s Coulomb criteria, the stress model discerned the state of the fractures, transforming the stress magnitudes into shear and effective normal stress on each fracture plane. Our observations indicated that the identified fractures existed in a noncritically stressed condition, suggesting a lack of interconnectivity among them. These findings correspond to the absence of gas production to date, providing insights into the dynamics of fractures and their impact on production behavior.
Automated, High-Throughput Platform to Generate a High-Reliability, Comprehensive Rectal Cancer Database
Authors: Bhutiani Neal , Yousef Mahmoud M.G. , Yousef Abdelrahman , Zeineddine Mohammad , Knafl Mark , Ratliff Olivia , Fernando Uditha P. , Turin Anastasia , Zeineddine Fadl A. , Jin Jeff , Alfaro-Munoz Kristin , Goldstein Drew , Chang George J. , Kopetz Scott , Shen John Paul , Uppal Abhineet 
Published: May 2024

PURPOSE Dynamic operations platforms allow for cross-platform data extraction, integration, and analysis, although application of these platforms to large-scale oncology enterprises has not been described. This study presents a pipeline for automated, high-fidelity extraction, integration, and validation of cross-platform oncology data in patients undergoing treatment for rectal cancer at a single, high-volume institution. METHODS A dynamic operations platform was used to identify patients with rectal cancer treated at MD Anderson Cancer Center between 2016 and 2022 who had magnetic resonance imaging (MRI) imaging and preoperative treatment details available in the electronic health record (EHR). Demographic, clinicopathologic, tumor mutation, radiographic, and treatment data were extracted from the EHR using a methodology adaptable to any disease site. Data accuracy was assessed by manual review. Accuracy before and after implementation of synoptic reporting was determined for MRI data. RESULTS A total of 516 patients with localized rectal cancer were included. In the era after institutional adoption of synoptic reports, the dynamic operations platform extracted T (tumor) category data from the EHR with 95% accuracy compared with 87% before the use of synoptic reports, and N (lymph node) category with 88% compared with 58%. Correct extraction of pelvic sidewall adenopathy was 94% compared with 78%, and extramural vascular invasion accuracy was 99% compared with 89%. Neoadjuvant chemotherapy and radiation data were 99% accurate for patients who had synoptic data sources. CONCLUSION Using dynamic operations platforms enables automated cross-platform integration of multiparameter oncology data with high fidelity in patients undergoing multimodality treatment for rectal cancer. These pipelines can be adapted to other solid tumors and, together with standardized reporting, can increase efficiency in clinical research and the translation of actionable findings toward optimizing patient outcomes.
The Traditional New Woman and Emerging New Man in Republican China
Authors: Lang Wang 
Published: May 2024

Abstract
This study focuses on the agony of married New Women, as well as the emerging New Man, in the 1920s and 1930s. While the New Woman in love has attracted considerable scholarly attention, the dilemma of New Women after marriage remains mostly ignored. Relying on literary works as well as articles in newspapers and periodicals, this essay attributes the New Woman's acute dissatisfaction to the uncontested gendered division of labor and the discontinuity between romantic relationships and institutionalized marriage. Intellectuals proposed communal childcare as the solution to the New Woman's dilemma; however, this still left the gendered labor division intact. This essay identifies the traditional role of the New Woman in the family and observes the emergence of xianfu liangfu (賢夫良父, “good husband and wise father”), a counterpart to xianqi liangmu (賢妻良母, “good wife and wise mother”). This understudied discourse demands equal responsibility at home and an attentive fatherhood from men.