bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioassay of Cisplatin and Doxorubicin by HeLa Cell Clonogenic Assay
Authors: Yoshimura Takahiro , Iwasaka Tsuyoshi , Kidera Yoshiro , Sugimori Hajime 
Published: August 2010

AbstractWith use of a new bioassay method, HeLa cell clonogenic assay, we examined the pharmacokinetics of cis‐diamminedichloroplatinum (CDDP) and Doxorubicin (DR) in vitro and in patients after administration of these antitumor agents. The in vitro study revealed that the inhibitory effects of both agents on colony growth were not only dose dependent but dependent on treatment time and that the inhibition of colony growth by CDDP decreased with the addition of fetal calf serum or human serum. The plasma samples were obtained from 9 patients with gynecological malignancies after administration of CDDP (35–50 mg/m2) or DR (35–50 mg/m2). The plasma levels of total CDDP and free CDDP were determined by atomic absorption assay, while DR was determined by fluoroassay. Plasma concentration of total CDDP gradually rose during continuous infusion, reaching a maximum at the end of the transfusion and slowly decreased thereafter. On the other hand concentrations of free CDDP increased according to total CDDP during continuous infusion and decreased rapidly after the completion of infusion. Antitumor activities in these plasmas measured by HeLa cell clonogenic assay correlated well with kinetics of free CDDP but not with total CDDP. The conclusion was that protein‐bound CDDP does not contribute to the inhibition of colony growth. Hence a bioassay of CDDP is considered to be important to know its true antitumor effects and to determine appropriate schedules for chemotherapy.
Term Pregnancy in a Rudimentary Horn of a Bicornuate Uterus with Foetal Salvage: A Case Report
Authors: Akhtar Asif Zia 
Published: August 2010

AbstractA case of pregnancy in the left rudimentary horn of a bicornuate uterus is described. The pregnancy was carried to term without the rupture of the horn and a live infant weighing 3.5 kg was delivered by lower segment caesarean section and thinned out horn of uterus was removed. The case is reported as it is one of the very few documented deliveries of a live‐born infant. Some of the factors associated with pregnancy in a rudimentary horn are discussed.
Role of Surgical Intervention in Severe Grade of Septic Abortions: An Analysis of 14 Cases
Authors: Pai Vidya , Dasan J. , Rao Usha R. , Pai U. S. , Nalini V. 
Published: August 2010

AbstractSeptic induced abortion is still posing a problem in our community. Out of 14 grade III septic induced abortion, 12 required surgical intervention as part of active management for appropriate therapy. Clinical variables like patient characteristics, method of induction, person responsible for induction, the methods, the reason for delay in admission were analysed in detail with a view to obtain a comprehensive idea of this socio‐medical problem and its prevention.
A Morphometric and Ultrastructural Study of the Microvessels of the Functional Zone of Normal Human Endometrium with Some Notions on Possible Secretory Functions of the Endothelial Cells
Authors: Blackwell Peter M. , Fraser Ian S. 
Published: August 2010

AbstractA comparative analysis of microvessel concentration in 24 specimens of normal human endometrium has been carried out in the mid‐proliferative (n = 12) and mid‐secretory (n = 12) phases of the menstrual cycle, with the finding of a significantly greater vascularity in the mid‐secretory phase (25.0+/‐12.7 microvessels/sq mm compared with 12.2+/‐6.3; mean +/‐SD; p<0.005).No significant difference was observed between the 2 phases in measurements of maximum luminal diameter, mean luminal diameter or luminal area, but there was a significantly greater mean diameter at the basement membrane in the proliferative compared with the secretory phase. This indicates that the microvessel diameter was larger in the proliferative phase due to greater height of the endothelial cells, while luminal size remained constant. Mean area (+/‐SD) of endothelial cell cytoplasm per transverse microvessel section was 58.5+/‐28.6 sq um in the mid‐proliferative phase and 44.9+/‐26.2 sq um in the mid‐secretory phase (p = 0.001).In a detailed ultrastructural study made of all the microvessels seen in 12 specimens of normal endometrium (6 mid‐proliferative and 6 mid‐secretory), the morphological appearance of the microvessels varied widely, especially with respect to cytoplasmic density and organelle concentration. Three distinct types of endothelial cell junction were observed each of which would impart a different level of cohesion to the vessel. A proposal is put forward that the presence of very weak junctions may be a factor leading to a propensity for excessive menstrual bleeding in some women.Endothelial cells have been shown by others to be highly metabolically active and secretory in nature and it has been assumed that they externalise their products by exocytotic secretory mechanisms.We have described unusual projections from the luminal membranes of human endometrial endothelial cells and free membrane‐bound luminal bodies observed in over a quarter of all endometrial microvessels. The function of these projections and bodies is unknown, but their appearances are consistent with the hypothesis that they represent a form of apocrine secretion with release of cytoplasmic material into the microvessel lumen for local and distant use.
The Effect of an Anti‐Progestin Compound (RU486) on Gonadotropin Release from Rat Anterior Pituitary Cells in vitro
Authors: Arakawa Satoko , Ohkawa Tomi , Kambegaaw Akira , Okinaga Shoichi , Arai Kiyoshi 
Published: August 2010

AbstractAnterior pituitary cells from castrated female rats were cultured in a medium with or without progesterone (P4), estradiol‐17 β (E2), 17 β‐hydroxy‐11 β‐(4‐dimethylaminophenyl)‐17‐(prop‐1‐ynyl)‐estra‐4,9‐dien‐3‐one (RU486) or norethisterone (NE). Three days later, the media were changed and the cells were incubated further with or without luteinizing hormone releasing hormone (LHRH) (10‐6‐10‐12 M) for 3 hours. LH and FSH released in the media were measured by radioimmunoassay (RIA).At a high concentration of LHRH (>10‐8 M), E2 (10‐9 M) increased the release of LH and FSH significantly. When P4 (10‐6 M) was combined with E2 for 3 days, the release of LH and FSH by E2 was inhibited.RU486 had no direct effect on LH and FSH release. However, the effect of P4 against E2, as mentioned above, was nullified by 10‐6 M of RU486.In the same experimental model, RU486 also inhibited the effect of norethisterone.By the use of the anti‐progestin compound, RU486, it was confirmed that progestins directly inhibited the action of E2 at the anterior pituitary level.
Premature Ovarian Failure: A Clinical, Histological and Cytogenetic Study
Authors: Tanaka Shoichi , Mizunuma Masahiro , Watanabe Hiroshi , Hata Hiroshi , Shimoya Yasuharu , Hashimoto Masayoshi 
Published: August 2010

AbstractIn this study, 17 cases of primary ovarian failure were studied clinically and endocrinologically. The average age at the beginning of amenorrhea was 23.7 years. The mean levels of serum LH, FSH and estradiol were 102.7 miu/ml, 99.6 miu/ml and 12.5 pg/ml, respectively. Chromosomal analysis was performed in 14 cases. One case was 46XXq– and one case 45X/46XY, while the others were categorized as 46XX karyotype. Ovarian biopsy was performed in 5 cases. In all 5 cases, no primordial follicle was found, but corpora albicantia were present, indicating past ovulation. Induction of ovulation was attempted in 16 cases. After estrogen progesterone cyclic therapy, one patient became pregnant, but gonadotropin therapy was ineffective. Although the etiology of primary ovarian failure remains unknown, pregnancy and ovulation can be achieved in some cases. With regard to treatment, estrogen replacement therapy may prove to be more effective than gonadotropin therapy.
Microinvasive Carcinoma of the Cervix and Colposcopy
Authors: Supreeya Wongtra‐Ngan , MacLean A. B. , Cordiner J. W. 
Published: August 2010

AbstractThe trainee in colposcopy is often taught that the colposcopic appearances of microinvasive carcinoma of the cervix can be distinguished from those due to intraepithelial neoplasia. However, our experience is that the colposcopic features of microinvasive carcinoma are not specific or diagnostic, and that the diagnosis from biopsy material is often unexpected.Atypical areas of the transformation zone should be biopsied during assessment before planning treatment. Any lesion that extends into the canal must be assessed by cone biopsy (cut by knife or laser). Providing occult invasion and canal involvement can be excluded, the interval between diagnosis and treatment is not prolonged, and the method of selective ablation ensures destruction to a depth of over 5 mm, the failure to recognise microinvasion in an area of intraepithelial neoplasia probably does not have clinical repercussions.
Eisenmenger's Syndrome with Pregnancy: A Rare Obstetrical Problem with Successful Outcome
Authors: Buckshee Kamal , Biswas Arijit , Mittal Suneeta , Agarwal Neera 
Published: August 2010

AbstractMore and more cases of congenital heart disease are reaching reproductive age and conceiving. Some of these do not pose much additional problem to the obstetrician but Eisenmenger's syndrome though rare is associated with a very high maternal mortality. The problem is further aggravated if patient develops pre‐eclampsia. Pregnancy termination is offered by most obstetricians when faced with a patient of Eisenmenger's syndrome with pregnancy. Few cases with successful outcome are reported in literature.
The Urodynamic Effect of Colposuspension Immediately after Surgery for Genuine Stress Incontinence
Authors: Wang Alex C. , Hsieh C. C. 
Published: August 2010

AbstractExtensive reports on postoperative urodynamic evaluation of female patients with GSI have been published in the current literature. However, urodynamic data from immediate postoperative evaluation has not yet been presented.Urodynamic study was made in 33 out of 41 patients from 9th–12th postoperative day. Our results indicated that preoperative and postoperative FUL in erect and supine position were 2.90±0.71; 3.72±0.98; P<0.005 and 2.13±0.68; 3.55±1.67; P<0.005 respectively. Preoperative PFR was 23.4±11.2 and postoperative PFR was 8.1±5.2; P<0.001. Preoperative RU was 69.9±83.3 and postoperative RU was 194±179; P<0.005.On the basis of the foregoing figures and our previous study, the following conclusions can be drawn; edematous swelling, possible hematoma formation and possible damage of the nerve supplying the bladder during dissection may be causes of postoperative voiding difficulty. The results of FUL obtained immediately following operation may serve as a parameter for predicting the outcome of corrective surgery. This may provide a simple way to judge whether or not the proximal urethra has been successfully restored to the intra‐abdominal pressure zone if the medical centers are not equipped with sophisticated instruments for measuring transmission ratios.
Outcome of Epileptic Pregnancy: Neonatal Hemorrhage due to Anticonvulsant Treatment of Mothers during Pregnancy
Authors: Ihara Yoshiyuki , Shimizu Takashi , Kawaguchi Kanetoshi , Inoue Kinya , Ando Nobuya 
Published: August 2010

AbstractSixteen neonates were born from epileptic women under anticonvulsant treatment. Most of the mothers were administered phenobarbital and phenytoin. Although no congenital anomaly was observed, 2 neonates showed hemorrhage and one died 25 hours after birth from profuse intra‐abdominal hemorrhage derived from ruptured subcapsular hematoma of the liver.The cord blood concentration of anticonvulsants correlated well with both maternal daily dose and maternal blood concentration. The ratio of cord blood to maternal blood concentration was approximately 1: 1 with phenobarbital and phenytoin. In the 2 neonates with hemorrhage, both the maternal dose and the cord blood concentration were relatively low.The cord blood of neonates born from an epileptic mother treated with anticonvulsants (especially phenobarbital and phenytoin), should immediately be submitted to coagulation tests, even if maternal daily dose or blood concentration of the drug is low. Such infants should be closely observed and be transfused with fresh frozen plasma, if bleeding is detected.
Long‐Term Immunoprophylaxis of Hepatitis B Surface Antigen Carrier in Infants Born to Hepatitis B Surface Antigen Positive Mothers Using Plasma Derived Vaccine
Authors: Theppisai Urusa , Thanuntaseth Chalermsri , Chiewsilp Pimol , Siripoonya Praput 
Published: August 2010

AbstractImmunoprophylaxis of HBV infection using 3 doses (10 μg) of plasma derived HBV vaccine were given to 49 newborn infants of e‐antigen negative HBs Ag carrier mothers at birth, one and 6 months of age. The protective efficacy rate was 100% during first 2 years. At 3 years of age, HBs Ag carrier was 2.63% and 89.48% of infants had anti‐HBs above the protective level. Ten point fifty‐two percent of total infants had anti‐HBs level below 10 mIU/ml and 21.05% had anti‐HBs level between 10–100 mIU/ml. Most infants who had the maximal level of antibody below 100 mIU/ml had lost all antibody within 3 years. The loss of antibody was closely related to the maximal anti‐HBs titer (one year of age) which is highly predictive indicator of the longevity of anti‐HBs.
Grandmultiparity: A Continuing Problem in Developing Countries
Authors: Karim Saadiya Aziz , Memon Abdul M. , Qadri Nighat 
Published: August 2010

AbstractFour hundred and thirty‐one grandmultiparae and 1,070 less parous women who delivered at Civil Hospital, Karachi over a 2‐year period (Jan. 1982 to Dec. 1983) have been reviewed retrospectively. The purpose was to estimate the incidence of grandmultiparity at this hospital and compare the incidence of obstetric and medical complications in the grandmultiparae with the less parous women. A higher incidence of anaemia, spontaneous abortions, abruptio placentae, postpartum haemorrhage, spontaneous uterine rupture and fetal loss was found in the grandmultiparae compared with other parturients. The grandmultipara continues to be a “high‐risk” obstetric problem.
An Experimental Study on the Effects of Elastase, Bleomycin and Infection on the Growth and Tensile Strength of Elastic Tissue in Rabbit Fetal Membranes
Authors: Chimura Tetsuro , Fujimori Katsuhiko 
Published: August 2010

AbstractThe effects of bleomycin, elastase and infection on the tensile strength of rabbit fetal membranes and the structure of the elastic tissue of rabbit amnion were studied. The experimental subjects were rabbits to which bleomycin (total dose, 20 mg) or elastase (total dose, 60 mg) was administered from 18 days of pregnancy. In addition to the fetal membranes from these treated animals, fetal membranes with amniotic infection induced by injection of Escherichia coli into the amniotic fluid (AF) of rabbits at 22 or 23 days of pregnancy were examined, and those from rabbits administered physiological saline solution were used as controls. The results obtained were as follows.Determination of tensile strength of the fetal membranes revealed that the infected membranes had minimum rupture pressure (18.67·6.30 mm Hg), followed by the elastase administration group (46.67·3.51 mm Hg). The value in the bleomycin administration group (99.67·12.89 mm Hg) was significantly higher than that in the control group (61.80·6.30 mm Hg). Histological changes in the fibroblast layer of these fetal membranes included elastic fibrosis due to excessive collagen production, which was mostly manifested as fibrosis densa, in the bleomycin administration group. In the elastase administration group, the elastic fibers were eliminated or became minute, as in the infection groups.The above results showed the adverse effects of elastase and bleomycin and the destructive effect of infection on the growth and properties of elastic tissue in the amnion, suggesting their pathophysiological importance.
Establishment and Characterization of CA 125 Producing Cell Line (OMC‐2) Originating from a Human Endometrial Adenocarcinoma
Authors: Yamada Takashi , Ueda Masatsugu , Maeda Takayoshi , Okamoto Yoshiaki , Miyawaki Yoshitaka , Otsuki Yoshinori , Ueki Minoru , Sugimoto Osamu 
Published: August 2010

AbstractA new human endometrial carcinoma cell line, designated OMC‐2, was established from the endometrial adenocarcinoma of a 59‐year‐old woman. This cell line has grown well for 51 months and has been subcultured more than 50 times. Monolayer cultured cells are polygonal in shape, showing a pavement‐like arrangement and a piling up tendency without contact inhibition. The chromosomal number shows aneuploidy and the modal chromosomal number is in the diploid range. The cells were transplanted into the subcutis of nude mice and produced tumors resembling the original tumor. 1X105 OMC‐2 cells produced CA 125 (184–682 U) during 19 days in culture media. CA 125 was demonstrated immunohistochemically in the original tumor, heterotransplanted tumor, and OMC‐2 cells. The cells contain no estrogen or progesterone receptors. Twenty‐nine other reports of endometrial carcinoma cell lines are reviewed.
Epidermal Growth Factor‐Like Immunoreactivity in Human Endometrium
Authors: Ishihara Satoko , Taketani Yuji , Mizuno Masahiko 
Published: August 2010

AbstractEpidermal growth factor (EGF)‐like immunoreactivity was detected in human endometrial tissues. The concentrations of the immunoreactivity fluctuated during the menstrual cycle. The highest levels were observed in the late proliferative phase with the concentrations being 0.50±0.06 ng/mg protein (M±SE, n=5). Considering the presence of EGF receptors in human endometrium, EGF‐like peptides may act in autocrine or paracrine fashions in human endometrium.
Thymidylate Synthase Inhibition as a Predictor of Tumor Sensitivity of Fluorinated Pyrimidines
Authors: Suzuki Mitsuaki , Sekiguchi Isao , Tamada Taro 
Published: August 2010

AbstractThe thymidylate synthase (TS) content of tumor tissue was assayed and the levels of 5‐fluorouracil (5‐Fu) in serum and tumor tissue were determined in 21 patients with cervical cancer who were treated with UFT, a fluorinated pyrimidine. Subrenal capsule assay, a predictive tumor sensitivity test, was conducted concurrently on cervical tumor samples. TS inhibition and serum and tissue 5‐Fu levels widely varied between both samples. Investigation of the relationship between the tumor growth inhibition rate determined by subrenal capsule assay and the above parameters (TS inhibition, serum and tissue 5‐Fu levels) showed that TS inhibition and tumor growth inhibition were well correlated (r=0.73). This suggests that a TS inhibition assay in tumor tissue can be a useful predictive test for tumor sensitivity of fluorinated pyrimidines.
Relationship between the Placental Location and the Flow Velocity Waveforms of Bilateral Uterine Arteries
Authors: Ito Yuji , Shouno Hideaki , Yamasaki Miyoshi , Oga Masayuki , Sugimori Hajime 
Published: August 2010

AbstractThe relationship between the placental location and the resistance index (RI) of the ascending branch of bilateral uterine arteries was examined with continuous Doppler ultrasonography, in 48 healthy pregnant women in the 37th to 40th week of gestation. When the placental location was central, there was little difference in the RI between the right and left uterine artery (fundus; 0.61±0.04 versus 0.58±0.03, NS, body; 0.48±0.04 versus 0.47±0.03, NS, and lower segment; 0.28±0.03 versus 0.30±0.04, NS). The RI became smaller when the level of placental attachment was lower toward the cervix. In cases where the placental location has shifted to the right or left of the uterine body, the RI on the placental side was smaller than that of the contralateral uterine artery (0.39±0.04 versus 0.62±0.03, p<0.01). The physiological and clinical significance of these results are discussed in the following report.
Augmentation of Labour by a Standard Protocol in Papua New Guinea
Authors: Mola Glen , Rageau One 
Published: August 2010

AbstractA study to investigate a policy of routine augmentation of dystocic labour by standard protocol encompassing definitions of delay in the first and second stages, dosage and administration of oxytocin, specific contra‐indications and monitoring progress of augmented labour.During the 5 months of the study 3,261 women were confined at Port Moresby General Hospital. In 329 of these labour was augmented by a standard regime. Amongst the 161 primigravidae thus augmented there were 8 (5%) caesarean sections and 34 (21.1%) assisted deliveries. Of the 166 multigravidae augmented there were 4 (2.4%) caesarean sections and 20 (12.1%) assisted deliveries. These figures can be compared against a total caesarean rate of 2.4% and an assisted delivery rate of 3% in 1988.Analysis of case controls did not show a significant difference in perinatal outcome for those who were augmented.We conclude that a standard management protocol for augmentation of labour in all cases of delay in the first and second stages of labour, excluding those with specific contra‐indications is safe and substantiates further the ascertion that active management of labour is a reasonable alternative to caesarean section for dystocia.
Immediate Postoperative Oral Hydration after Caesarean Section
Authors: Guedj Pierre , Eldor Joseph , Stark Michael 
Published: August 2010

AbstractA study was carried out to assess the effects of immediate postoperative oral rehydration in 51 unpremedicated women undergoing caesarean section under epidural anesthesia. The patients were randomly assigned to 2 groups: group 1 (n=22)—fasting at least until 24 hours after the end of the operation, and group 2 (n=29)—receiving immediate oral intake of fluids (water, tea or coffee with sugar) without limitation of quantity. The 2 groups were compared for the occurrence of postoperative nausea and vomiting, onset of peristalsis, rectal gas emission, first bowel movement, and possible complications. The results demonstrate no significant differences between the parturients who drank immediately postoperatively as compared to those in whom oral fluid intake was delayed for 24 hours or more. It is concluded that immediate postoperative oral rehydration had no harmful effect upon peristalsis post‐caesarean section.
Risk Determination in Cases with an Extra Minute Chromosome: Prenatal Diagnosis
Authors: Sago Haruhiko , Iinuma Kazuso , Yoshiwara Sachiko , Takeda Tomoko , Takeda Osamu , Onda Takekazu , Kitagawa Michihiro , Komuro Nobuyoshi , Terashima Yoshiteru , Kinoshita Hideo 
Published: August 2010

AbstractThe interpretation of an extra minute chromosome (EMC) detectedin uteropresents both diagnostic and prognostic problems. Two EMC cases are presented and a flow‐chart guideline is proposed for the determination of developmental risk. A prenatally detected EMC was familially inherited through a normal phenotype father. The pregnancy was continued and a normal baby boy was delivered. At one year his development was within normal ranges. The second EMC case was a mentally retarded girl examined at 4 years and 7 months of age. The EMC was identified with a combination of various staining techniques to be an inversion duplication (15). The importance of differential diagnosis of EMCs by DA/DAPI staining is emphasized.