bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reducing costs in flow‐cytometric counting of residual white blood cells in blood products: utilization of a single‐platform bead‐free flow‐rate calibration method
Authors: Fischer Johannes C. , Quenzel Ernst M. , Moog Rainer , Wenzel Folker , Riethmacher Regina , Tutschek Boris , Giers Günther 
Published: January 2011

BACKGROUND: Commercial flow‐cytometric methods for counting residual white blood cells (rWBCs) in leukoreduced blood products use calibration beads for estimation of the measured sample volume. A bead‐free flow‐rate calibration method is developed and validated.STUDY DESIGN AND METHODS: The analyzed volume was calculated by acquisition time (ACQ). Twenty‐nine spiking series of red blood cell (RBC) or platelet (PLT) products were prepared containing levels ranging from 0.08 × 106 up to 2048 × 106 WBCs/L. Nearly WBC‐free triple‐leukofiltered RBCs or PLT concentrates (PCs) served as background. Propidium iodide (PI) was used to identify rWBCs. Five RBC series were compared against a commercially available kit (LeukoSure, Beckman Coulter). Routine capabilities were tested on 41 RBC and 92 PC samples of two independent transfusion services.RESULTS: The lower detection limit in RBC was 0.08 × 106 rWBCs/L for ACQ and 0.16 for LeukoSure. Criteria for linearity, accuracy, and precision were fulfilled within the range of 0.5 × 106 to 512 × 106 WBCs/L. For PCs, all these criteria were fulfilled between 0.5 × 106 and 32 × 106 rWBCs/L (lower detection limit of 0.25) for PI. ACQ and LeukoSure agreed sufficiently (81%) when tested on routine RBCs or PCs.CONCLUSION: A residual WBC count of fewer than 0.5 × 106 WBCs/L can be accurately counted using the ACQ approach at a total reagent cost of less than 0.5€ per sample.
Down regulation of human natural killer cell–mediated cytolysis induced by blood transfusion: role of transforming growth factor‐β1, soluble Fas ligand, and soluble Class I human leukocyte antigen
Authors: Ghio Massimo , Contini Paola , Negrini Simone , Mazzei Clemente , Zocchi Maria R. , Poggi Alessandro 
Published: January 2011

BACKGROUND: Human natural killer (NK) cells are thought to play a role in antiviral response and tumor immune surveillance. The molecular mechanisms of down regulation of NK‐cell activity observed after red blood cell (RBC) transfusion is still undefined.STUDY DESIGN AND METHODS: Both effects of blood transfusion (ex vivo) and supernatants (SNs) derived from RBC units unstored (RBC‐0) or stored for 5 or 30 days (RBC‐5 or ‐30, respectively) in vitro were analyzed on NK cell–mediated cytolytic activity.RESULTS: We have found that NK cells isolated from transfused patients on Day 3 lysed the NK‐sensitive target cells K562 to a lesser extent than before transfusion. This down regulation of NK‐cell activation was evident also for NK‐cell killing mediated through the engagement of NK cell–activating receptors as NKG2D, NKp30, NKp46, and CD16. Transfused patients reacquired NK cell–mediated cytolytic activity from Day 5 to Day 7 after transfusion. SN from RBC‐30, but not from RBC‐0 or RBC‐5, strongly inhibited the generation of lymphokine‐activated killer (LAK) cells and lysis of the NK‐resistant target cell Jurkat in a dose‐dependent manner. Transforming growth factor‐β1 (TGF‐β1) blocking antibodies partially restored the generation of LAK activity. In addition, the depletion of both soluble Class I human leukocyte antigens (sHLA‐I) and soluble Fas ligand (sFasL) from SN of RBC‐30 completely restored the generation of LAK activity.CONCLUSIONS: Altogether, these findings would support the idea that blood transfusion–mediated down regulation of NK‐cell activity is mediated by sHLA‐I, sFasL, and TGF‐β1.
Use of hemovigilance data to evaluate the effectiveness of diversion and bacterial detection
Authors: Robillard Pierre , Delage Gilles , Karl Itaj Nawej , Goldman Mindy 
Published: January 2011

BACKGROUND: Several preventive measures, including diversion of the first aliquot of blood and culturing of platelet (PLT) components, have been implemented to decrease the risk of transfusion‐transmitted bacterial infections (TTBIs). We evaluated the effectiveness of these measures in Québec using hemovigilance data from January 2000 to December 2008.STUDY DESIGN AND METHODS: Adverse transfusion reactions were reported to the Québec Ministry of Health by transfusion safety officers. Initial aliquot diversion, already in place for apheresis PLTs, was added to all whole blood collections in early 2003. Bacterial detection was implemented in March 2003 for apheresis PLTs and February 2005 for whole blood–derived PLTs (WBDPs).RESULTS: The incidence of probable and definite TTBIs associated with WBDPs decreased from 1 in 2655 to 1 in 27,737 five‐unit pools (p = 0.004) after implementation of diversion. There were no reports of TTBIs with WBDPs after culture was added to diversion, further reducing the risk to 1 in 58,123 five‐unit pools (p < 0.001). There was only one TTBI associated with apheresis PLTs during the 9‐year period, which occurred after implementation of both diversion and culture.CONCLUSION: Hemovigilance data demonstrated a highly significant decrease in TTBIs associated with WBDPs, mainly attributed to the implementation of diversion. However, diversion and culture do not totally abolish the risk of TTBIs.
Donation frequency of blood donors participating in a prospective cohort study of iron status
Authors: Bahrami Shrein H. , Guiltinan Anne M. , Schlumpf Karen S. , Scott Erik , Banks Linda L. , D'Andrea Pam , Hartman Elizabeth L. , Vij Vibha , Wright David J. , Spencer Bryan , Murphy Edward L. ,   for the NHLBI Retrovirus Epidemiology Donor Study‐II (REDS‐II)
Published: January 2011

BACKGROUND: Blood centers are interested in understanding determinants of frequent blood donation. We hypothesized that participation in uncompensated research could result in higher donation rates.STUDY DESIGN AND METHODS: Donation rates for 2425 subjects from six US blood centers enrolled in the Retrovirus Epidemiology Donor Study‐II Donor Iron Status Evaluation Study were compared to those of nonenrolled donors (n = 202,383). Over 15 months, we compared mean donation rates and adjusted rate ratios (RRs) between enrolled and nonenrolled for three subgroups, first‐time, reactivated, and frequent donors, and donation rates before and after the study enrollment period for frequent donors only.RESULTS: Enrolled donors had higher 15‐month mean donation rates than nonenrolled donors (first‐time, 1.21 [RR = 1.91]; reactivated, 1.68 [RR = 1.83]; frequent, 3.40 [RR = 1.12]). However, frequent donors donated at approximately the same rate after enrollment as they did before enrollment in the study (3.62 per 15 months [RR = 1.12]).CONCLUSION: Donors enrolled in the study donated at a higher rate than nonenrolled donors, but frequent donors remained consistent in their donation frequency both before and after enrollment. Although increased donation rates could have been causally related to study enrollment, we cannot rule out an enrollment bias whereby more committed donors were more likely to enroll in the study.
Allergic transfusion reactions to platelets are associated more with recipient and donor factors than with product attributes
Authors: Savage William J. , Tobian Aaron A. , Fuller Alice K. , Wood Robert A. , King Karen E. , Ness Paul M. 
Published: January 2011

BACKGROUND: Mechanisms of allergic transfusion reactions (ATRs) are not well understood. The aim of this study was to distinguish recipient‐, donor‐, and product‐specific factors associated with ATRs.STUDY DESIGN AND METHODS: We conducted a retrospective cohort study of apheresis platelet (AP) products transfused from April 2000 through March 2010. The concordance rate of ATRs when split AP products were transfused to at least two individuals was compared to the overall ATR rate among all AP products. Per‐person ATR rates also were compared to the overall ATR rate.RESULTS: We observed 1616 ATRs among 93,737 transfusions, for an overall incidence of 1.72% (95% confidence interval [CI], 1.64%‐1.81%). Of the 1616 ATRs, 630 occurred when split AP products were transfused to at least two recipients. Of these 630 AP products, ATRs were observed in at least two different recipients of the same AP collection only 6 of 630 times, for a concordant incidence of 0.95% (95% CI, 0.35%‐2.06%), which is similar to the overall ATR rate (p = 0.17). On an individual level, 30.0% of recipients had ATR rates of more than 5%, and these 30.0% accounted for 62.1% of ATRs. Donors of AP products associated with concordant ATRs donated AP products that had an ATR rate of 5.8% (95% CI, 3.1%‐9.7%), which is higher than the overall ATR rate (p < 0.001).CONCLUSIONS: An observed ATR does not predict an ATR in a different recipient of a split AP product. A minority of platelet recipients accounts for the majority of ATRs. Some donors are strongly associated with ATRs. Consequently, recipient and donor factors are implicated in the mechanism of ATRs.
Matrix Metalloproteinase Activity Causes VEGFR‐2 Cleavage and Microvascular Rarefaction in Rat Mesentery
Authors: TRAN EDWARD D. , YANG MING , CHEN ANDREW , DELANO FRANK A. , MURFEE WALTER L. , SCHMID‐SCHÖNBEIN GEERT W. 
Published: January 2011

Please cite this paper as: Tran, Yang, Chen, DeLano, Murfee and Schmid‐Schönbein (2011). Matrix Metalloproteinase Activity Causes VEGFR‐2 Cleavage and Microvascular Rarefaction in Rat Mesentery. Microcirculation 18(3), 228–237.AbstractA complication of the spontaneously hypertensive rat (SHR) is microvascular rarefaction, defined by the loss of microvessels. However, the molecular mechanisms involved in this process remain incompletely identified. Recent work in our laboratory suggests that matrix metalloproteinases (MMPs) may play a role by cleavage of the vascular endothelial growth factor receptor 2 (VEGFR‐2). In order to further delineate the role for MMPs in microvascular rarefaction, the objective of the current study was to examine the relationship in the same tissue between MMP activity, VEGFR‐2 cleavage and rarefaction. Using an in vivo microzymographic technique, we show significantly enhanced levels of MMP‐1, ‐1/‐9, ‐7, and ‐8 activities, but not MMP‐2 and ‐3 activities, along mesenteric microvessels of the SHR compared to its normotensive control, Wistar Kyoto rat. Based on immunohistochemical methods, the SHR exhibited a decreased labeling of the extracellular, but not the intracellular, domain of VEGFR‐2 along mesenteric microvessels. Chronic MMP inhibition served to attenuate VEGFR‐2 cleavage and microvascular network rarefaction in the SHR mesentery. These results spatially link MMP‐induced VEGFR‐2 cleavage and rarefaction in the mesentery of the SHR and thus support the hypothesis that MMPs serve as regulators of microvascular dysfunction in hypertension.
Wound healing and regenerative strategies
Authors: Nauta A , Gurtner GC , Longaker MT 
Published: January 2011

Oral Diseases (2011) 17, 541–549Wound healing is a complex biological process that affects multiple tissue types. Wounds in the oral cavity are particularly challenging given the variety of tissue types that exist in close proximity to one another. The goal of regenerative medicine is to facilitate the rapid replacement of lost or damaged tissue with tissue that is functional, and physiologically similar to what previously existed. This review provides a general overview of wound healing and regenerative medicine, focusing specifically on how recent advances in the fields of stem cell biology, tissue engineering, and oral disease could translate into improved clinical outcomes.
Bounded Ethicality in Negotiations1
Authors: Bazerman Max H. 
Published: January 2011

AbstractMost negotiators think of themselves as good people, and most negotiators act in ethically questionable ways at times. How can these two descriptions be reconciled? This paper follows Bazerman and Tenbrunsel (2011) in arguing that good people often engage in unethical acts without their own awareness of doing so. This paper specifically explores how negotiators may be prejudiced, favor in‐groups, and overclaim in negotiation, without knowing that they are doing anything wrong.
When Does Taking a Break Help in Negotiations? The Influence of Breaks and Social Motivation on Negotiation Processes and Outcomes
Authors: Harinck Fieke , De Dreu Carsten K. W. 
Published: January 2011

AbstractMost negotiations are interrupted from time to time to reflect on the negotiation or to do other pressing tasks. This study investigated how these breaks and the thoughts during these breaks influence subsequent negotiation behavior. Prosocially motivated dyads, with a tendency to think cooperatively, and proself‐motivated dyads, with a tendency to think competitively, engaged in a negotiation in which there was a 3‐min break. During this break, either they could reflect upon the negotiation or they did a distraction task. After the reflect break, but not after the distract break, prosocial dyads displayed more concern for other, engaged in more cooperative negotiation, and reached more agreements than proself dyads. We conclude that breaks are constructive if, and only if, negotiators reflect on their negotiation when having a prosocial orientation.
Controlled search term vocabularies for finding articles relevant to injury prevention and safety promotion
Authors: Lawrence David W 
Published: January 2011

ObjectivesTo assess the usefulness of the controlled vocabularies of PubMed/MEDLINE and PsycINFO for finding articles on injury prevention and safety promotion (IPSP) topics and to identify specific indexing problems that can contribute to incomplete retrieval.MethodsProfessional reference librarians provided search strategies for finding articles relevant to five topics pertaining to the injury prevention field in the two bibliographic databases. The results of implementing these search strategies were compared with the results of a presumptive gold standard—serial textword searches on the same topics. The index terms assigned to the articles that were missed by the librarian strategies were examined.ResultsThe search products of the librarian-constructed search strategies identified 34–91% of the IPSP-relevant articles that were identified through serial textword searches of the two databases. Specific indexing issues were found to contribute to this loss.ConclusionsLibrarians bring expertise to searching, but irregular or incomplete indexing can limit the product of even well-constructed searches for articles on IPSP topics.
Method for simple and rapid enumeration of total epiphytic bacteria in the washing solution of rice plants
Authors: Niwa Rieko , Yoshida Shigenobu , Furuya Naruto , Tsuchiya Kenichi , Tsushima Seiya 
Published: January 2011

The phyllosphere is one of the most common habitats for terrestrial bacteria. However, little is known about the populations of bacteria, including unculturable bacteria, that thrive on plant surfaces. Here, we developed a fluorescent nuclear staining technique to easily and rapidly observe and enumerate populations of total and living epiphytic bacteria, with particular emphasis on the concentration by centrifugation and fixation of the epiphytic bacteria. An investigation on the optimal conditions for centrifugation and fixation revealed that centrifugation at 20 400g for 2 min and fixation with 0.5% glutaraldehyde solution were the optimum conditions for observation of the bacteria. Using this technique, we assessed the populations of the total and living bacteria on the surface of rice plants. When epiphytic bacteria were recovered from rice seeds ( Oryza sativa ‘Koshihikari’), the number of total and living bacterial cells was 7.36 and 6.85 log10·g–1(fresh mass) in the seed washing, respectively. In contrast, the numbers of total and living bacterial cells in the leaf sheath washings were 5.5–5.8 and 5.3–5.7 log10·g–1, respectively. Approximately 5%–30% of the total bacteria in the washing solution of rice plant were culturable. The usefulness of the enumeration method and the amount of bacteria on the plant surfaces are discussed.
miR-99 Family of MicroRNAs Suppresses the Expression of Prostate-Specific Antigen and Prostate Cancer Cell Proliferation
Authors: Sun Dandan , Lee Yong Sun , Malhotra Ankit , Kim Hak Kyun , Matecic Mirela , Evans Clive , Jensen Roderick V. , Moskaluk Christopher A. , Dutta Anindya 
Published: January 2011

Abstract
MicroRNAs (miRNA) have been globally profiled in cancers but there tends to be poor agreement between studies including in the same cancers. In addition, few putative miRNA targets have been validated. To overcome the lack of reproducibility, we profiled miRNAs by next generation sequencing and locked nucleic acid miRNA microarrays and verified concordant changes by quantitative RT-PCR. Notably, miR-125b and the miR-99 family members miR-99a, -99b, and -100 were downregulated in all assays in advanced prostate cancer cell lines relative to the parental cell lines from which they were derived. All four miRNAs were also downregulated in human prostate tumor tissue compared with normal prostate. Transfection of miR-99a, -99b, or -100 inhibited the growth of prostate cancer cells and decreased the expression of prostate-specific antigen (PSA), suggesting potential roles as tumor suppressors in this setting. To identify targets of these miRNAs, we combined computational prediction of potential targets with experimental validation by microarray and polyribosomal loading analysis. Three direct targets of the miR-99 family that were validated in this manner were the chromatin-remodeling factors SMARCA5 and SMARCD1 and the growth regulatory kinase mTOR. We determined that PSA is posttranscriptionally regulated by the miR-99 family members, at least partially, by repression of SMARCA5. Together, our findings suggest key functions and targets of miR-99 family members in prostate cancer suppression and prognosis. Cancer Res; 71(4); 1313–24. ©2011 AACR.
Targeting Activin Receptor-Like Kinase 1 Inhibits Angiogenesis and Tumorigenesis through a Mechanism of Action Complementary to Anti-VEGF Therapies
Authors: Hu-Lowe Dana D. , Chen Enhong , Zhang Lianglin , Watson Katherine D. , Mancuso Patrizia , Lappin Patrick , Wickman Grant , Chen Jeffrey H. , Wang Jianying , Jiang Xin , Amundson Karin , Simon Ronald , Erbersdobler Andreas , Bergqvist Simon , Feng Zheng , Swanson Terri A. , Simmons Brett H. , Lippincott John , Casperson Gerald F. , Levin Wendy J. , Stampino Corrado Gallo , Shalinsky David R. , Ferrara Katherine W. , Fiedler Walter , Bertolini Francesco 
Published: January 2011

Abstract
Genetic and molecular studies suggest that activin receptor-like kinase 1 (ALK1) plays an important role in vascular development, remodeling, and pathologic angiogenesis. Here we investigated the role of ALK1 in angiogenesis in the context of common proangiogenic factors [PAF; VEGF-A and basic fibroblast growth factor (bFGF)]. We observed that PAFs stimulated ALK1-mediated signaling, including Smad1/5/8 phosphorylation, nuclear translocation and Id-1 expression, cell spreading, and tubulogenesis of endothelial cells (EC). An antibody specifically targeting ALK1 (anti-ALK1) markedly inhibited these events. In mice, anti-ALK1 suppressed Matrigel angiogenesis stimulated by PAFs and inhibited xenograft tumor growth by attenuating both blood and lymphatic vessel angiogenesis. In a human melanoma model with acquired resistance to a VEGF receptor kinase inhibitor, anti-ALK1 also delayed tumor growth and disturbed vascular normalization associated with VEGF receptor inhibition. In a human/mouse chimera tumor model, targeting human ALK1 decreased human vessel density and improved antitumor efficacy when combined with bevacizumab (anti-VEGF). Antiangiogenesis and antitumor efficacy were associated with disrupted co-localization of ECs with desmin+ perivascular cells, and reduction of blood flow primarily in large/mature vessels as assessed by contrast-enhanced ultrasonography. Thus, ALK1 may play a role in stabilizing angiogenic vessels and contribute to resistance to anti-VEGF therapies. Given our observation of its expression in the vasculature of many human tumor types and in circulating ECs from patients with advanced cancers, ALK1 blockade may represent an effective therapeutic opportunity complementary to the current antiangiogenic modalities in the clinic. Cancer Res; 71(4); 1362–73. ©2011 AACR.
The Role of Calcium in the Activation of Estrogen Receptor-Alpha
Authors: Divekar Shailaja D. , Storchan Geoffrey B. , Sperle Katherine , Veselik David J. , Johnson Earl , Dakshanamurthy Sivanesan , Lajiminmuhip Yuse N. , Nakles Rebecca E. , Huang Li , Martin Mary Beth 
Published: January 2011

Abstract
Environmental estrogen mimics, including metalloestrogens that can activate estrogen receptor-alpha (ERα), may contribute to breast cancer risk. However, the underlying mechanisms through which these molecular mimics activate the ERα are generally poorly understood. With concern to this important question, we investigated whether intracellular calcium may mediate the cross-talk between signaling pathways that activate ERα and the ligand-binding domain of ERα. MCF-7 cells treated with EGF, ATP, extracellular calcium, or caffeine to increase intracellular calcium triggered a rapid recruitment of ERα to estrogen-responsive promoters and stimulated expression of estrogen-responsive genes including pS2, complement C3, and progesterone receptor. Induction was blocked by an antiestrogen but also by the chelation of intracellular calcium. Treatment with extracellular calcium also increased the growth of MCF-7 cells through an ER-dependent mechanism. We found that EGF and extracellular calcium activated the C-terminus of ERα and the activation was blocked by the antiestrogen. Mechanistic investigations identified four potential sites on the solvent-accessible surface of the ERα ligand-binding domain as important for calcium activation of the receptor. Taken together, our results suggest that calcium mediates the cross-talk between ERα-activating signaling pathways and the ligand-binding domain of ERα providing a potential explanation for the ability of certain environmental metalloestrogens to activate the receptor. Cancer Res; 71(5); 1658–68. ©2011 AACR.
Increased Efficacy of Breast Cancer Chemotherapy in Thrombocytopenic Mice
Authors: Demers Mélanie , Ho-Tin-Noé Benoit , Schatzberg Daphne , Yang Janie J. , Wagner Denisa D. 
Published: January 2011

Abstract
Platelets contribute to homeostasis of the tumor vasculature by helping prevent hemorrhage. Thus, we hypothesized that inducing thrombocytopenia would increase tumor vascular leakiness and facilitate the effective delivery of chemotherapeutic agents to tumors. In a mammary carcinoma murine model, platelet depletion induced bleeding specifically at the tumor site, favoring the accumulation of fluorescently labeled microspheres only in the tumor. Moreover, induction of thrombocytopenia in tumor-bearing mice before injection of paclitaxel increased its intratumoral accumulation and reduced growth of both slow- and fast-growing tumors, compared with mice with normal platelet counts that were treated only with paclitaxel. Histologic analysis confirmed the expectation of an increase in tumor apoptosis and a reduction in tumor proliferation in thrombocytopenic mice receiving chemotherapy. No increased toxicity was seen in other organs or blood cells. Taken together, our results indicate that low platelet count selectively induces leakiness of tumor vessels and favors the delivery of chemotherapy to tumor sites, enhancing its tumoricidal effects. Cancer Res; 71(5); 1540–9. ©2011 AACR.