bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enabling Advanced Snow Physics Within Land Surface Models Through an Interoperable Model‐Physics Coupling Framework
Authors: Navari Mahdi , Kumar Sujay , Wang Shugong , Geiger James , Mocko David M. , Arsenault Kristi R. , Kemp Eric M. 
Published: April 2024

AbstractAccurate estimation of snow accumulation and melt is a critical part of decision‐making in snow‐dominated watersheds. In this study, we demonstrate a flexible methodology to couple a detailed snow model, Crocus, separately to two different land surface models (LSMs), Noah‐MP and Noah. The original LSMs and the coupled models (Noah‐MP‐Crocus and Noah‐Crocus) are used to simulate snow depth, snow water equivalent, and other water and energy states and fluxes. The results of simulations are compared against a wide range of independent gridded and point scale reference data sets. Our results show that coupling the detailed snow model, Crocus, with the LSMs improves the snow depth and snow water equivalent relative to independent observations. Overall, larger improvements are obtained with coupling Crocus to the Noah LSM, with the coupled Noah‐Crocus configuration reducing the RMSE and bias of snow depth from 2% to 12% and 57% to 75%, respectively, relative to Snow Data Assimilation System (SNODAS) and snow product from the University of Arizona. On the other hand, smaller improvements are obtained by coupling Crocus with Noah‐MP. The Coupled Noah‐MP‐Crocus reduces the snow depth bias but slightly degrades the RMSE of snow depth and snow water equivalent. The corresponding impacts in other water budget terms such as evapotranspiration, soil moisture, and streamflow, however, are mixed, pointing to the significant need to improve the coupling assumptions of these processes within land models. Overall, the interoperable coupling framework demonstrated here offers the opportunity to include more detailed snow physics and processes, and to advance data assimilation systems through improved exploitation of information from snow remote sensing instruments.
Anthropocene North Western Pacific Oceanography Recorded as Seasonal‐Resolution Radiocarbon in Coral From Kikai Island, Japan
Authors: Zeng Yuning , Yokoyama Yusuke , Hirabayashi Shoko , Miyairi Yosuke , Suzuki Atsushi , Aze Takahiro , Kawakubo Yuta 
Published: April 2024

AbstractRadiocarbon (14C) in corals can be used as a relatively high‐sensitivity indicator of vertical and horizontal advection of water masses, which contributes to the understanding of ocean circulation. In this study, we reconstruct Kuroshio and Ryukyu current transport with a seasonal resolution Δ14C record spanning 1947–2009. This record covers the beginning of the atomic era and was obtained from a coral on Kikai Island in the south of Japan. The Kikai Δ14C curve features a newly discovered Δ14C spike in July 1955, a rapid increase after 1962, and a steady decrease after 1980. The spike in 1955 may directly reflect ocean current transport. The lack of periodicity in the Δ14C record suggests the existence of mesoscale eddies and the complexity of Kuroshio and Ryukyu current transport. In addition, comparing the high‐resolution Δ14C of Kikai and Ishigaki islands, both situated along the path of the Kuroshio, reveals the influence of Pacific Decadal Oscillation and El Niño‐Southern Oscillation on the Kuroshio and Ryukyu currents. This suggests that seasonally resolved Δ14C in corals along an ocean current can produce a long‐term record of ocean mixing that responds to climate variability.
Sub‐MeV Electron Precipitation Driven by EMIC Waves Through Nonlinear Fractional Resonances
Authors: Hanzelka M. , Li W. , Qin M. , Capannolo L. , Shen X. , Ma Q. , Gan L. , Angelopoulos V. 
Published: April 2024

AbstractElectromagnetic ion cyclotron waves in the Earth's outer radiation belt drive rapid electron losses through wave‐particle interactions. The precipitating electron flux can be high in the hundreds of keV energy range, well below the typical minimum resonance energy. One of the proposed explanations relies on nonresonant scattering, which causes pitch‐angle diffusion away from the fundamental cyclotron resonance. Here we propose the fractional sub‐cyclotron resonance, a second‐order nonlinear effect that scatters particles at resonance order n = 1/2, as an alternate explanation. Using test‐particle simulations, we evaluate the precipitation ratios of sub‐MeV electrons for wave packets with various shapes, amplitudes, and wave normal angles. We show that the nonlinear sub‐cyclotron scattering produces larger ratios than the nonresonant scattering when the wave amplitude reaches sufficiently large values. The ELFIN CubeSats detected several events with precipitation ratio patterns matching our simulation, demonstrating the importance of sub‐cyclotron resonances during intense precipitation events.
Using Vehicle‐Induced DAS Signals for Near‐Surface Characterization With High Spatiotemporal Resolution
Authors: Yuan Siyuan , Liu Jingxiao , Noh Hae Young , Clapp Robert , Biondi Biondo 
Published: April 2024

AbstractVehicle‐induced seismic waves, generated as vehicles traverse the ground surface, carry valuable information for imaging the underlying near‐surface structure. These waves propagate differently in the subsurface depending on soil properties at various spatial locations. By leveraging wave propagation characteristics, such as surface‐wave velocity and attenuation, this study presents a novel method for near‐surface monitoring. Our method employs passing vehicles as active, non‐dedicated seismic sources and leverages pre‐existing telecommunication fibers as large‐scale and cost‐effective roadside sensors empowered by Distributed Acoustic Sensing (DAS) technology. A specialized Kalman filter algorithm is integrated for automated DAS‐based traffic monitoring to accurately determine vehicles' location and speed. Then, our approach uniquely leverages vehicle trajectories to isolate space‐time windows containing high‐quality surface waves. With known vehicle (i.e., seismic source) locations, we can effectively mitigate artifacts associated with suboptimal distribution of sources in conventional ambient noise interferometry. Compared to ambient noise interferometry, our approach enables the synthesis of virtual shot gathers with a high signal‐to‐noise ratio and spatiotemporal resolution at reduced computational costs. We validate the effectiveness of our method using the Stanford DAS‐2 array, with a focus on capturing spatial heterogeneity and monitoring temporal variations in soil seismic properties during rainfall events. Specifically, in non‐built‐up areas, we observed an evident decrease in phase velocity and group velocity and an increase in attenuation due to the rainfall. Our findings illustrate our method's sensitivity and resolution in discerning variations across different spatial locations and demonstrate that our method is a promising advancement for high‐resolution near‐surface imaging in urban settings.
Protective effect of increased O-GlcNAc cycling against 6-OHDA induced Parkinson’s disease pathology
Authors: Kim Dong Yeol , Kim Sang-Min , Cho Eun-Jeong , Kwak Hyo-Bum , Han Inn-Oc 
Published: April 2024

AbstractThis study aimed to elucidate the role of O-GlcNAc cycling in 6-hydroxydopamine (6-OHDA)-induced Parkinson’s disease (PD)-like neurodegeneration and the underlying mechanisms. We observed dose-dependent downregulation of O-GlcNAcylation, accompanied by an increase in O-GlcNAcase following 6-OHDA treatment in both mouse brain and Neuro2a cells. Interestingly, elevating O-GlcNAcylation through glucosamine (GlcN) injection provided protection against PD pathogenesis induced by 6-OHDA. At the behavioral level, GlcN mitigated motor deficits induced by 6-OHDA, as determined using the pole, cylinder, and apomorphine rotation tests. Furthermore, GlcN attenuated 6-OHDA-induced neuroinflammation and mitochondrial dysfunction. Notably, augmented O-GlcNAcylation, achieved through O-GlcNAc transferase (OGT) overexpression in mouse brain, conferred protection against 6-OHDA-induced PD pathology, encompassing neuronal cell death, motor deficits, neuroinflammation, and mitochondrial dysfunction. These collective findings suggest that O-GlcNAcylation plays a crucial role in the normal functioning of dopamine neurons. Moreover, enhancing O-GlcNAcylation through genetic and pharmacological means could effectively ameliorate neurodegeneration and motor impairment in an animal model of PD. These results propose a potential strategy for safeguarding against the deterioration of dopamine neurons implicated in PD pathogenesis.
Inter- and trans-generational impacts of real-world PM2.5 exposure on male-specific primary hypogonadism
Authors: Wei Xiaoyu , Zhang Zhonghao , Gu Yayun , Zhang Rong , Huang Jie , Li Feng , He Yuanlin , Lu Shuai , Wu Yifei , Zeng Wentao , Liu Xiaorui , Liu Chenzi , Liu Jinyi , Ao Lin , Shi Fuquan , Chen Qing , Lin Yuan , Du Jiangbo , Jin Guangfu , Xia Yankai , Ma Hongxia , Zheng Yuxin , Huo Ran , Cao Jia , Shen Hongbing , Hu Zhibin 
Published: April 2024

AbstractExposure to PM2.5, a harmful type of air pollution, has been associated with compromised male reproductive health; however, it remains unclear whether such exposure can elicit transgenerational effects on male fertility. Here, we aim to examine the effect of paternal exposure to real-world PM2.5 on the reproductive health of male offspring. We have observed that paternal exposure to real-world PM2.5 can lead to transgenerational primary hypogonadism in a sex-selective manner, and we have also confirmed this phenotype by using an external model. Mechanically, we have identified small RNAs (sRNAs) that play a critical role in mediating these transgenerational effects. Specifically, miR6240 and piR016061, which are present in F0 PM sperm, regulate intergenerational transmission by targeting Lhcgr and Nsd1, respectively. We have also uncovered that piR033435 and piR006695 indirectly regulate F1 PM sperm methylation by binding to the 3′-untranslated region of Tet1 mRNA. The reduced expression of Tet1 resulted in hypermethylation of several testosterone synthesis genes, including Lhcgr and Gnas, impaired Leydig cell function and ultimately led to transgenerational primary hypogonadism. Our findings provide insights into the mechanisms underlying the transgenerational effects of paternal PM2.5 exposure on reproductive health, highlighting the crucial role played by sRNAs in mediating these effects. The findings underscore the significance of paternal pre-conception interventions in alleviating the adverse effects of environmental pollutants on reproductive health.
Rare coinheritance of hemoglobin vancleave with severe beta-thalassemia mutation in a patient with secondary erythrocytosis
Authors: Aziz Nur Aisyah , Musa Nurul Hidayah , Mathews Melina , Rajenderan Komalah Thevii , Abdul Hamid Faidatul Syazlin , Hassan Syahzuwan , Omar Syahira Lazira , Wan Yusoff Wan Nurul Afiqha Binti , Mohd Din Melanie Ling Binti , Jamaludin Nurul Amira Binti , Wan Taib Wan Rohani , Esa Ezalia , Mohd Yasin Norafiza 
Published: April 2024

Abstract
Hemoglobin (Hb) Vancleave (NM_000518.5:c.431 A > T; dbSNP: rs33918338) is an extremely rare structural hemoglobin variant worldwide, and studies are limited. This report describes the case of a 16-year-old male patient who presented with secondary erythrocytosis. The diagnosis of Hb Vancleave, in combination with codon 41/42 (-TTCT) (NM_000518.5:c.126_129del; dbSNP: rs80356821), was confirmed by direct sequencing. This report highlights the importance of sequencing in the differential diagnosis of beta-thalassemia syndrome in Malaysia.
Brightness illusions drive a neuronal response in the primary visual cortex under top-down modulation
Authors: Saeedi Alireza , Wang Kun , Nikpourian Ghazaleh , Bartels Andreas , Logothetis Nikos K. , Totah Nelson K. , Watanabe Masataka 
Published: April 2024

AbstractBrightness illusions are a powerful tool in studying vision, yet their neural correlates are poorly understood. Based on a human paradigm, we presented illusory drifting gratings to mice. Primary visual cortex (V1) neurons responded to illusory gratings, matching their direction selectivity for real gratings, and they tracked the spatial phase offset between illusory and real gratings. Illusion responses were delayed compared to real gratings, in line with the theory that processing illusions requires feedback from higher visual areas (HVAs). We provide support for this theory by showing a reduced V1 response to illusions, but not real gratings, following HVAs optogenetic inhibition. Finally, we used the pupil response (PR) as an indirect perceptual report and showed that the mouse PR matches the human PR to perceived luminance changes. Our findings resolve debates over whether V1 neurons are involved in processing illusions and highlight the involvement of feedback from HVAs.
Dynamic acoustic optimization of pulse tube refrigerators for rapid cooldown
Authors: Snodgrass Ryan , Kotsubo Vincent , Backhaus Scott , Ullom Joel 
Published: April 2024

AbstractPulse tube refrigerators are a critical enabling technology for many disciplines that require low temperatures. These refrigerators dominate the total power consumption of most modern cryostats, including those that reach millikelvin temperatures using additional cooling stages. In state-of-the-art commercial pulse tube refrigerators, the acoustic coupling between the driving compressor and the refrigerator is fixed and optimized for operation at base temperature. We show that this optimization is incorrect during the cooldown process, which results in wasted power consumption by the compressor and slow cooldown speed. After developing analytic expressions that demonstrate the need for acoustic tuning as a function of temperature, we dynamically optimize the acoustics of a commercial pulse tube refrigerator and show that the cooldown speed can be increased to 1.7 to 3.5 times the original value. Acoustic power measurements show that loss mechanism(s)—and not the capacity of the compressor—limit the maximum cooling available at high temperatures, suggesting that even faster cooldown speeds can be achieved in the future. This work has implications for the accessibility of cryogenic temperatures and the cadence of research in many disciplines such as quantum computing.
Exosome-coated oxygen nanobubble-laden hydrogel augments intracellular delivery of exosomes for enhanced wound healing
Authors: Han Xiaoxue , Saengow Chaimongkol , Ju Leah , Ren Wen , Ewoldt Randy H. , Irudayaraj Joseph 
Published: April 2024

AbstractWound healing is an obvious clinical concern that can be hindered by inadequate angiogenesis, inflammation, and chronic hypoxia. While exosomes derived from adipose tissue-derived stem cells have shown promise in accelerating healing by carrying therapeutic growth factors and microRNAs, intracellular cargo delivery is compromised in hypoxic tissues due to activated hypoxia-induced endocytic recycling. To address this challenge, we have developed a strategy to coat oxygen nanobubbles with exosomes and incorporate them into a polyvinyl alcohol/gelatin hybrid hydrogel. This approach not only alleviates wound hypoxia but also offers an efficient means of delivering exosome-coated nanoparticles in hypoxic conditions. The self-healing properties of the hydrogel, along with its component, gelatin, aids in hemostasis, while its crosslinking bonds facilitate hydrogen peroxide decomposition, to ameliorate wound inflammation. Here, we show the potential of this multifunctional hydrogel for enhanced healing, promoting angiogenesis, facilitating exosome delivery, mitigating hypoxia, and inhibiting inflammation in a male rat full-thickness wound model.
Peripheral immune cells in metastatic breast cancer patients display a systemic immunosuppressed signature consistent with chronic inflammation
Authors: Chauhan Sudhir Kumar , Dunn Claire , Andresen Nikolai Kragøe , Røssevold Andreas Hagen , Skorstad Gjertrud , Sike Adam , Gilje Bjørnar , Raj Sunil Xavier , Huse Kanutte , Naume Bjørn , Kyte Jon Amund 
Published: April 2024

AbstractImmunotherapies blocking the PD-1/PD-L1 checkpoint show some efficacy in metastatic breast cancer (mBC) but are often hindered by immunosuppressive mechanisms. Understanding these mechanisms is crucial for personalized treatments, with peripheral blood monitoring representing a practical alternative to repeated biopsies. In the present study, we performed a comprehensive mass cytometry analysis of peripheral blood immune cells in 104 patients with HER2 negative mBC and 20 healthy donors (HD). We found that mBC patients had significantly elevated monocyte levels and reduced levels of CD4+ T cells and plasmacytoid dendritic cells, when compared to HD. Furthermore, mBC patients had more effector T cells and regulatory T cells, increased expression of immune checkpoints and other activation/exhaustion markers, and a shift to a Th2/Th17 phenotype. Furthermore, T-cell phenotypes identified by mass cytometry correlated with functionality as assessed by IFN-γ production. Additional analysis indicated that previous chemotherapy and CDK4/6 inhibition impacted the numbers and phenotype of immune cells. From 63 of the patients, fresh tumor samples were analyzed by flow cytometry. Paired PBMC-tumor analysis showed moderate correlations between peripheral CD4+ T and NK cells with their counterparts in tumors. Further, a CD4+ T cell cluster in PBMCs, that co-expressed multiple checkpoint receptors, was negatively associated with CD4+ T cell tumor infiltration. In conclusion, the identified systemic immune signatures indicate an immune-suppressed environment in mBC patients who had progressed/relapsed on standard treatments, and is consistent with ongoing chronic inflammation. These activated immuno-suppressive mechanisms may be investigated as therapeutic targets, and for use as biomarkers of response or treatment resistance.
Genomic landscape of diploid and aneuploid microsatellite stable early onset colorectal cancer
Authors: Zhou Yumei , Chen Xianfeng , Chen Jun , Kendrick Conner D. , Ramanathan Ramesh K. , Graham Rondell P. , Kossick Kimberlee F. , Boardman Lisa A. , Barrett Michael T. 
Published: April 2024

AbstractAlthough colorectal cancer (CRC) remains the second leading cause of cancer-related death in the United States, the overall incidence and mortality from the disease have declined in recent decades. In contrast, there has been a steady increase in the incidence of CRC in individuals under 50 years of age. Hereditary syndromes contribute disproportionately to early onset CRC (EOCRC). These include microsatellite instability high (MSI+) tumors arising in patients with Lynch Syndrome. However, most EOCRCs are not associated with familial syndromes or MSI+ genotypes. Comprehensive genomic profiling has provided the basis of improved more personalized treatments for older CRC patients. However, less is known about the basis of sporadic EOCRC. To define the genomic landscape of EOCRC we used DNA content flow sorting to isolate diploid and aneuploid tumor fractions from 21 non-hereditary cases. We then generated whole exome mutational profiles for each case and whole genome copy number, telomere length, and EGFR immunohistochemistry (IHC) analyses on subsets of samples. These results discriminate the molecular features of diploid and aneuploid EOCRC and provide a basis for larger population-based studies and the development of effective strategies to monitor and treat this emerging disease.
Induction of altered states of consciousness during Floatation-REST is associated with the dissolution of body boundaries and the distortion of subjective time
Authors: Hruby Helena , Schmidt Stefan , Feinstein Justin S. , Wittmann Marc 
Published: April 2024

AbstractFloatation-REST (Reduced Environmental Stimulation Therapy) minimizes stimulation of the nervous system by immersing subjects in an environment without sound or light while they effortlessly float in thermoneutral water supersaturated with Epsom salt. Here we investigated the relationship between altered states of consciousness (ASC) and its association with the affective changes induced by Floatation-REST. Using a within-subject crossover design, 50 healthy subjects were randomized to 60 min of Floatation-REST or 60 min of Bed-REST (an active control condition that entailed lying supine on a warm waterbed in a dark and quiet room). Following Floatation-REST, subjects felt significantly more relaxed, less anxious, and less tired than after Bed-REST. Floatation-REST also induced significantly more pronounced ASC characterized by the dissolution of body boundaries and the distortion of subjective time. The loss of body boundaries mediated the loss of anxiety, revealing a novel mechanism by which Floatation-REST exerts its anxiolytic effect.
Concordance of multigene genealogy along with morphological evidence unveils five novel species and two new records of boletoid mushrooms (fungi) from India
Authors: Das Kanad , Ghosh Aniket , Datta Sudeshna , Singh Upendra , Chakraborty Dyutiparna , Tudu Debala , Vizzini Alfredo 
Published: April 2024

AbstractAgaricales, Russulales and Boletales are dominant orders among the wild mushrooms in Basidiomycota. Boletaceae, one of the major functional elements in terrestrial ecosystem and mostly represented by ectomycorrhizal symbionts of trees in Indian Himalaya and adjoining hills, are extraordinarily diverse and represented by numerous genera and species which are unexplored or poorly known. Therefore, their hidden diversity is yet to be revealed. Extensive macrofungal exploration by the authors to different parts of Himalaya and surroundings, followed by through morphological studies and multigene molecular phylogeny lead to the discovery of five new species of wild mushrooms: Leccinellum bothii sp. nov., Phylloporus himalayanus sp. nov., Phylloporus smithii sp. nov., Porphyrellus uttarakhandae sp. nov., and Retiboletus pseudoater sp. nov. Present communication deals with morphological details coupled with illustrations and phylogenetic inferences. Besides, Leccinellum sinoaurantiacum and Xerocomus rugosellus are also reported for the first time from this country.