bluuebunny/crossref_metadata_2025_split huggingface dataset:
Causal relationships between gut microbiota and lymphoma: a bidirectional Mendelian randomization study
Authors: Liang Jing , Liu Gengqiu , Wang Wenqing , Xue Hongman 
Published: May 2024

BackgroundMultiple studies have suggested a possible connection between the gut microbiota and the development of lymphoma, though the exact nature of this relationship remains unclear. This study aimed to explore whether a causal association exists between gut microbiota and lymphoma.MethodsA bidirectional two-sample Mendelian randomization (MR) approach was conducted to investigate potential causal effects between gut microbiota and various lymphoma subtypes. The primary method employed for MR analysis was inverse variance weighted (IVW), supplemented by additional methods including MR-Egger, weighted median, and weighted mode approaches. The Cochrane Q test, MR-PRESSO global test and MR-Egger intercept test were performed to assess pleiotropy and heterogeneity. Furthermore, a reverse MR analysis was performed to explore potential reverse causal effect.ResultsThe primary MR analysis identified 36 causal relationships between genetic liabilities in gut microbiota and different lymphoma subtypes. Neither the MR-PRESSO test nor the MR-Egger regression detected any pleiotropy, and Cochran’s Q test indicated no significant heterogeneity.ConclusionsOur MR analysis revealed substantial causal associations between gut microbiota and lymphoma, offering new insights into lymphoma prevention and management microbiota.
Plasmodium falciparum AMA1 and CSP antigen diversity in parasite isolates from southern Ghana
Authors: Kusi Kwadwo A. , Amoah Linda E. , Acquah Festus Kojo , Ennuson Nana Aba , Frempong Abena F. , Ofori Ebenezer A. , Akyea-Mensah Kwadwo , Kyei-Baafour Eric , Osei Frank , Frimpong Augustina , Singh Susheel K. , Theisen Michael , Remarque Edmond J. , Faber Bart W. , Belmonte Maria , Ganeshan Harini , Huang Jun , Villasante Eileen , Sedegah Martha 
Published: May 2024

IntroductionDiversity in malarial antigens is an immune evasion mechanism that gives malaria parasites an edge over the host. Immune responses against one variant of a polymorphic antigen are usually not fully effective against other variants due to altered epitopes. This study aimed to evaluate diversity in the Plasmodium falciparum antigens apical membrane antigen 1 (PfAMA1) and circumsporozoite protein (PfCSP) from circulating parasites in a malaria-endemic community in southern Ghana and to determine the effects of polymorphisms on antibody response specificity.MethodsThe study involved 300 subjects, whose P. falciparum infection status was determined by microscopy and PCR. Diversity within the two antigens was evaluated by msp2 gene typing and molecular gene sequencing, while the host plasma levels of antibodies against PfAMA1, PfCSP, and two synthetic 24mer peptides from the conserved central repeat region of PfCSP, were measured by ELISA. ResultsOf the 300 subjects, 171 (57%) had P. falciparum infection, with 165 of the 171 (96.5%) being positive for either or both of the msp2 allelic families. Gene sequencing of DNA from 55 clonally infected samples identified a total of 56 non-synonymous single nucleotide polymorphisms (SNPs) for the Pfama1 gene and these resulted in 44 polymorphic positions, including two novel positions (363 and 365). Sequencing of the Pfcsp gene from 69 clonal DNA samples identified 50 non-synonymous SNPs that resulted in 42 polymorphic positions, with half (21) of these polymorphic positions being novel. Of the measured antibodies, only anti-PfCSP antibodies varied considerably between PCR parasite-positive and parasite-negative persons. DiscussionThese data confirm the presence of a considerable amount of unique, previously unreported amino acid changes, especially within PfCSP. Drivers for this diversity in the Pfcsp gene do not immediately seem apparent, as immune pressure will be expected to drive a similar level of diversity in the Pfama1 gene.
Non-linear association and benchmark dose of blood pressure on carotid artery intima-media thickening in a general population of southern China
Authors: Qin Linyuan , Wu Xiaoyan , Tan Chao , Zhang Zhengbao , Li You , Zhu Xiaonian , Qin Shenghua , Tan Shengkui 
Published: May 2024

Background and aimsThis study aimed to evaluate whether there is a J-curve association between blood pressure (BP) and carotid artery intima-media thickening (CAIT) and estimate the effect of the turning point of BP on CAIT.Methods and resultsData from 111,494 regular physical examinations conducted on workers and retirees (aged 18 years or older) between January 2011 and December 2016, exported from the hospital information system, were analyzed. Restricted cubic splines (RCS) logistic regression was employed to access the association of BP with CAIT, and Bayesian benchmark dose methods were used to estimate the benchmark dose as the departure point of BP measurements. All the pnon-linear values of BP measurements were less than 0.05 in the RCS logistic regression models. Both systolic blood pressure (SBP) and diastolic blood pressure (DBP) had J-curve associations with the risk of CAIT at a turning point around 120/70 mmHg in the RCS. The benchmark dose for a 1% change in CAIT risk was estimated to be 120.64 mmHg for SBP and 72.46 mmHg for DBP.ConclusionThe J-curve associations between SBP and DBP and the risk of CAIT were observed in the general population in southern China, and the turning point of blood pressure for significantly reducing the risk of CAIT was estimated to be 120.64/72.46 mmHg for SBP/DBP.
Infant action understanding: the roles of active training and motor development
Authors: Chung Haerin , Filippi Courtney A. , Woodward Amanda L. 
Published: May 2024

IntroductionThis study examined the potential interplay between motor development and intervention in support of action understanding.MethodsEighty nine-month-old infants completed a tool-use training session and goal imitation paradigm that assessed action understanding in counterbalanced order. A metric of motor development was obtained using the Early Motor Questionnaire.ResultsResults indicated that training improved action understanding, particularly for those infants who started out with lower means-end skills. Results further indicated that infants who did not receive any training experience in the lab beforehand, drew on their existing means-end skills.DiscussionThese results emphasize independent contributions of training and motor development on action understanding and shed light on the interaction between training and individual motor readiness in facilitating action understanding in infancy.
Sedimentary records of giant landslide-dam breach events in western Sichuan, China
Authors: Ma Junxue , Chen Jian , Xu Chong 
Published: May 2024

Introduction: Landslide-dammed lake outburst floods (LLOFs) are common natural disasters in high-mountain regions, posing serious safety threats to residents’ livelihoods and properties and causing major damage to engineering facilities. Giant landslides and river damming events commonly occur in the Eastern Tibetan Plateau in southwestern China. Dam failure generate LLOFs that form outburst deposits (ODs). This phenomenon is particularly common in some giant ancient landslide-dammed lakes.Methods: This study conducted a detailed investigation of the sedimentary characteristics of large-scale landslide-dammed lake outburst deposits in the Diexi Reach of the Upper Minjiang River and Tangjiashan Reach of the Tongkou River Basin, West Sichuan Plateau, China. Meanwhile, typical evidence of high-energy ODs was recorded.Results and Discussion: The longitudinal distribution of these ODs is similar to an elongated fan-shaped terrace along the river channel, presenting a distinctive sedimentary disordered–sub-ordered–ordered sequence from upstream to downstream. Several typical units of “sedimentary facies” are developed in the OD profiles, such as boulder units deposited by high-energy outburst flood (OF) events and the gravel and sand units representing pulsating-flow sedimentary environments during the recession stage. The grain size frequency curves are bimodal, and the granularity accumulated curves are upward convex, which reflect that the detrital characteristics of the sediment source area are mainly composed of coarse gravel and boulders. This indicates that the coarse gravel sediment gradually become decreased from upstream to downstream. Moreover, the OD hydrodynamic intensity displays a gradual weakening, and sediment sorting is improved. From upstream to the downstream, the mean particle-size and sorting of the ODs gradually decrease. The skewness become larger, and the kurtosis of the ODs is distributed in all the types. In addition, the different combinations of quartz sand surface microtextures indicate the transformation from high-to low-energy impacts over a short distance, which is controlled by flood hydrodynamics and regime.Significance: These sedimentary characteristics of ODs explain the hydrodynamic changes during the propagation of OFs, and are also important records for distinguishing between ODs, and “normal” floods.
Genome-wide identification of the walnut MYC gene family and functional characterization of Xinjiang wild walnut under low-temperature stress
Authors: Song Ya-Ting , Ma Kai , Zhao Yu , Han Li-Qun , Liu Li-Qiang 
Published: May 2024

Introduction: MYC transcription factors are the basic regulators of the jasmonic acid signaling pathway and play important roles in plant growth and development and the response to adverse stress. In recent years, severe winter freezing and late spring frost in the main planting area of walnut in Xinjiang have affected the growth and development of walnut, which has become a prominent problem restricting walnut production. Xinjiang wild walnut is the only remaining wild species of walnuts in China, which contains a lot of genes with excellent traits, and is important for the cultivation and breeding.Methods: In this paper, the physicochemical properties and bioinformatics of MYC transcription factor members in walnut were analyzed, and the nine MYC were screened from the transcriptome data under low temperature stress. At last, we study the subcellular localizations and the expression patterns of the nine MYC members in Xinjiang wild walnut.Results: The results revealed that 30 MYC members were identified from published walnut whole-genome data, and their evolutionary relationships with Arabidopsis and poplar were divided into six groups according to clustering analysis, among which JrMYC22 and JrMYC23 had high homology with PtrMYC2b, which is induced by jasmonic acid in response to low-temperature stress. Walnut MYC members are unevenly distributed on 12 chromosomes. The prediction of promoter cis-acting elements of walnut MYC transcription factor family members revealed that cis-acting elements related to jasmonic acid and lowtemperature stress were the ones with the greatest number of members, with 12. In addition, all nine MYC family members in Xinjiang wild walnut plants responding to low-temperature stress exhibited strong fluorescence responses in the nucleus. The expression levels of these members in response to low-temperature stress revealed that JrMYC28, JrMYC31, JrMYC33, JrMYC34, and JrMYC35 were highly expressed, and it was hypothesized that JrMYC28, JrMYC31, JrMYC33, JrMYC34, and JrMYC35 might play a key role in the response to lowtemperature stress.Discussion: The results of this study provide a theoretical basis for further research on the functional mechanisms of the MYC transcription factor family members in walnut.
Study on ice resistance of Antarctic krill ship with trawl under floating ice sea conditions
Authors: Xiong Zhixin , Wu Xinyuan , Guo Yu , Fu Zheng 
Published: May 2024

IntroductionThis study focused on a Chinese Antarctic krill vessel utilising continuous pumping fishing technology. The resistance characteristics of Antarctic krill ships trawling in floating ice areas is of great significance for the navigation and fishing of krill ships in ice areas.MethodsFirstly, MATLAB programming using discrete elements combined with genetic algorithms was used to construct a normal distribution ice flow model. Secondly, a fluid-structure coupling interface is created through the contact between the fluid and the trawl grid, and the displacement and resistance of the trawl grid are evaluated on the shared interface. Finally, the effects of ice density and ship sailing speed on ice resistance were studied.Results and discussionThe results of the calculations results show that ice resistance is positively related to the concentration and speed of floating ice, moreover, there is a special speed point where ice resistance increases rapidly. As the speed increases, the proportion of trawl resistance to the total resistance continues to increase, while the proportion of ice resistance continues to decrease. This paper provides a reference for the navigation and fishing resistance assessment of Antarctic krill ships in floating ice areas.
Anesthesia strategies for elderly patients with craniocerebral injury due to foreign-body penetration in the plateau region: a case report
Authors: Sun Yongtao , Liu Yang , Liu Peng , Zhang Min , Liu Mengjie , Wang Yuelan 
Published: May 2024

BackgroundThe administration of anesthesia for elderly individuals who are critically ill, suffering from severe craniocerebral injuries, and living in plateau regions presents a rare, intricate, and high-risk challenge. This case study outlines the specific anesthesia management protocols necessary for plateau-dwelling patients with significant craniocerebral damage undergoing prolonged invasive procedures.Case reportA 76-year-old male patient had a 26-year history of foreign-body penetration of the skull and had experienced local purulent discharge and pain for the previous 20 days. The diagnoses included right hypoplasia, a foreign body in the skull with an infection, hypokalemia, hypoproteinemia, pulmonary fibrous foci, and bilateral pleural effusion. For almost 6 months, the patient suffered from recurring headaches, blurred vision, and sluggish bodily movement. The patient had a poor diet, poor sleep quality, normal urination, and no noticeable weight loss since the onset of the illness. The right anterior ear had a 2 cm skin abscess with yellow pus and a black metal foreign body tip. The left eyelid was red and swollen, and the left conjunctiva was hyperemic; the right eyelid showed no abnormalities, and both pupils were wide and round, with light and adjustment reflexes and no cyanosis on the lips. Skull development was normal. No dry or moist rales were audible in either lung. The heart rhythm was regular, and the heart rate was 50 bpm. Chest CT revealed left lung calcification foci, bilateral pleural effusion, and fiber foci in the lower lobes of both lungs.ConclusionFurthermore, the patient in question was of advanced age and had a complex medical history, including prolonged exposure to high altitudes and previous instances of severe craniocerebral trauma, among other uncommon pathophysiological characteristics. In particular, the patient also underwent surgical interventions at both high and low altitudes, adding to the complexity of their case. To ensure patient safety, close multidisciplinary collaboration, the development of a precise surgical plan, and the implementation of a suitable perioperative anesthetic management strategy are imperative.
Bio-fabricated zinc oxide nanoparticles mediated by endophytic fungus Aspergillus sp. SA17 with antimicrobial and anticancer activities: in vitro supported by in silico studies
Authors: Abdelrahman Sally El Said Abo Halawa , El Hawary Seham , Mohsen Engy , El Raey Mohamed A. , Selim Heba Mohammed Refat M. , Hamdan Ahmed M. E. , Ghareeb Mosad A. , Hamed Ahmed A. 
Published: May 2024

IntroductionIn recent years, the world’s attention has been drawn to antimicrobial resistance (AMR) because to the frightening prospect of growing death rates. Nanomaterials are being investigated due to their potential in a wide range of technical and biological applications.MethodsThe purpose of this study was to biosynthesis zinc oxide nanoparticles (ZnONPs) using Aspergillus sp. SA17 fungal extract, followed by characterization of the produced nanoparticles (NP) using electron microscopy (TEM and SEM), UV-analysis, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FT-IR).Results and DiscussionThe HR-TEM revealed spherical nanoparticles with an average size of 7.2 nm, and XRD validated the crystalline nature and crystal structure features of the generated ZnONPs, while the zeta potential was 18.16 mV, indicating that the particles’ surfaces are positively charged. The FT-IR was also used to identify the biomolecules involved in the synthesis of ZnONPs. The antibacterial and anticancer properties of both the crude fungal extract and its nano-form against several microbial strains and cancer cell lines were also investigated. Inhibition zone diameters against pathogenic bacteria ranged from 3 to 13 mm, while IC50 values against cancer cell lines ranged from 17.65 to 84.55 M. Additionally, 33 compounds, including flavonoids, phenolic acids, coumarins, organic acids, anthraquinones, and lignans, were discovered through chemical profiling of the extract using UPLC-QTOF-MS/MS. Some molecules, such pomiferin and glabrol, may be useful for antibacterial purposes, according to in silico study, while daidzein 4’-sulfate showed promise as an anti-cancer metabolite.
Antifungal activity of Carica papaya fruit extract against Microsporum canis: in vitro and in vivo study
Authors: Aljuhani Salma , Rizwana Humaira , Aloufi Abeer S. , Alkahtani Saad , Albasher Gadah , Almasoud Hadeel , Elsayim Rasha 
Published: May 2024

BackgroundTinea capitis (T. capitis), commonly known as scalp ringworm, is a fungal infection affecting the scalp and hair. Among the causative agents, Microsporum canis (M. canis) stands out, often transmitted from cats to humans (zoonotic disease). In this study, we investigated the efficacy of Carica papaya (C. papaya), fruit extract against dermatophytes, particularly M. canis, both in vitro and in vivo. Additionally, we aimed to identify the active compounds responsible for suppressing fungal growth and assess the toxicity of C. papaya on human cells.MethodologyIt conducted in two parts. First, In Vitro Study include the preparation of C. papaya fruit extract using methanol as the solvent, Phytochemical analysis of the plant extract including Gas chromatography–mass spectrometry (GC–MS) and Fourier-transform infrared spectroscopy (FTIR) was conducted, Cytotoxicity assays were performed using HUH-7 cells, employing the MTT assay (1-(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide), Antimicrobial activity against M. canis was evaluated, including: Zone of inhibition (ZI), Minimum inhibitory concentration (MIC), Minimum fungicidal concentration (MFC), M. canis cell alterations were observed using scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Second, In Vivo, Albino Wistar male rats were included.ResultsThe phytochemical analysis of the methanolic extract from papaya revealed several functional groups, including hydroxyl, ammonia, alkane, carbonate, and alcohol. Additionally, the GC–MS analysis identified 15 compounds, with xanthosine and decanoic acid being the predominant components. The methanolic extract of papaya fruits demonstrated potent antifungal activity: ZI = 37 mm, MIC = 1,000 μg/mL, MFC = 1900 μg/mL, MTT results indicated lower cytotoxicity of the fruit extract at concentrations of 20 μg/mL, 50 μg/mL, 100 μg/mL, 150 μg/mL, and 200 μg/mL, The IC50 revealed a significant decrease in cell viability with increasing extract concentration. Notably, papaya extract induced considerable alterations in the morphology of M. canis hyphae and spores. In animal tissue, improvements were observed among the group of rats which treated with Papaya extract. This study highlights the potential of C. papaya fruits as a natural antifungal agent, warranting further exploration for clinical applications.
Valine induces inflammation and enhanced adipogenesis in lean mice by multi-omics analysis
Authors: Zheng Hui-Yi , Wang Li , Zhang Rong , Ding Ran , Yang Cai-Xia , Du Zhi-Qiang 
Published: May 2024

IntroductionThe branched-chain amino acids (BCAAs) are essential to mammalian growth and development but aberrantly elevated in obesity and diabetes. Each BCAA has an independent and specific physio-biochemical effect on the host. However, the exact molecular mechanism of the detrimental effect of valine on metabolic health remains largely unknown.Methods and resultsThis study showed that for lean mice treated with valine, the hepatic lipid metabolism and adipogenesis were enhanced, and the villus height and crypt depth of the ileum were significantly increased. Transcriptome profiling on white and brown adipose tissues revealed that valine disturbed multiple signaling pathways (e.g., inflammation and fatty acid metabolism). Integrative cecal metagenome and metabolome analyses found that abundances of Bacteroidetes decreased, but Proteobacteria and Helicobacter increased, respectively; and 87 differential metabolites were enriched in several molecular pathways (e.g., inflammation and lipid and bile acid metabolism). Furthermore, abundances of two metabolites (stercobilin and 3-IAA), proteins (AMPK/pAMPK and SCD1), and inflammation and adipogenesis-related genes were validated.DiscussionValine treatment affects the intestinal microbiota and metabolite compositions, induces gut inflammation, and aggravates hepatic lipid deposition and adipogenesis. Our findings provide novel insights into and resources for further exploring the molecular mechanism and biological function of valine on lipid metabolism.
Differentiation of benign and malignant parotid gland tumors based on the fusion of radiomics and deep learning features on ultrasound images
Authors: Wang Yi , Gao Jiening , Yin Zhaolin , Wen Yue , Sun Meng , Han Ruoling 
Published: May 2024

ObjectiveThe pathological classification and imaging manifestation of parotid gland tumors are complex, while accurate preoperative identification plays a crucial role in clinical management and prognosis assessment. This study aims to construct and compare the performance of clinical models, traditional radiomics models, deep learning (DL) models, and deep learning radiomics (DLR) models based on ultrasound (US) images in differentiating between benign parotid gland tumors (BPGTs) and malignant parotid gland tumors (MPGTs).MethodsRetrospective analysis was conducted on 526 patients with confirmed PGTs after surgery, who were randomly divided into a training set and a testing set in the ratio of 7:3. Traditional radiomics and three DL models (DenseNet121, VGG19, ResNet50) were employed to extract handcrafted radiomics (HCR) features and DL features followed by feature fusion. Seven machine learning classifiers including logistic regression (LR), support vector machine (SVM), RandomForest, ExtraTrees, XGBoost, LightGBM and multi-layer perceptron (MLP) were combined to construct predictive models. The most optimal model was integrated with clinical and US features to develop a nomogram. Receiver operating characteristic (ROC) curve was employed for assessing performance of various models while the clinical utility was assessed by decision curve analysis (DCA).ResultsThe DLR model based on ExtraTrees demonstrated superior performance with AUC values of 0.943 (95% CI: 0.918-0.969) and 0.916 (95% CI: 0.861-0.971) for the training and testing set, respectively. The combined model DLR nomogram (DLRN) further enhanced the performance, resulting in AUC values of 0.960 (95% CI: 0.940- 0.979) and 0.934 (95% CI: 0.876-0.991) for the training and testing sets, respectively. DCA analysis indicated that DLRN provided greater clinical benefits compared to other models.ConclusionDLRN based on US images shows exceptional performance in distinguishing BPGTs and MPGTs, providing more reliable information for personalized diagnosis and treatment plans in clinical practice.