bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antioxidant capacity and environmentally friendly synthesis of dihydropyrimidin‐(2H)‐ones promoted by naturally occurring organic acids
Authors: de Vasconcelos Alana , Oliveira Pathise S. , Ritter Marina , Freitag Rogério A. , Romano Renato L. , Quina Frank H. , Pizzuti Lucas , Pereira Claudio M. P. , Stefanello Francieli M. , Barschak Alethéa G. 
Published: March 2012

AbstractThe Biginelli reaction is a multicomponent reaction involving the condensation between an aldehyde, a β‐ketoester, and urea or thiourea, in the presence of an acid catalyst, producing dihydropyrimidinones (DHPMs). Owing to their important pharmacological properties, the DHPMs have been studied by many authors. However, most of the methodologies used for the synthesis of these compounds require drastic reaction conditions. In the current study, we report an efficient and clean procedure for preparing DHPMs by the use of citric acid or tartaric acid as a promoter of the Biginelli synthesis in ethanol as solvent. In addition, we have evaluated the antioxidant capacity of the compounds synthesized by the 2,2‐diphenyl‐1‐picrylhydrazyl radical scavenging assay and the thiobarbituric acid‐reactive species test. Two compounds presented antioxidant activity and also reduced lipid peroxidation at concentrations of 200 and 300 µM. In summary, we report an environmentally friendly procedure for the preparation of DHPMs and demonstrate the antioxidant capacity of some of the compounds. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:155–161, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20424
Insulin‐Like growth factor I receptor involvement in proliferation of NOR‐P1 cells in serum‐free media
Authors: Tomizawa Minoru , Shinozaki Fuminobu , Sugiyama Takao , Yamamoto Shigenori , Sueishi Makoto , Yoshida Takanobu 
Published: March 2012

AbstractInsulin‐like growth factor (IGF)‐I is up‐regulated in pancreatic cancer tissues. Pancreatic cancer cell lines were analyzed in serum‐free media as a model of the fibrous tissues that these cells often invade. Pancreatic cancer surgical specimens were immunostained with anti‐IGF‐I receptor (IGF‐IR)β antibody. The growth of pancreatic cancer cells in serum‐free media was also analyzed. Cell lysates were analyzed for protein by western blot analysis. Cells cultured in the presence of picropodophyllin (PPP), LY294002, or PD98059, were subjected to cell proliferation and scratch assays. In addition, BrdU uptake and apoptosis were analyzed in these cells. IGF‐IRβ was detected in pancreatic cancer cells invading fibrous tissues. NOR‐P1 grew most rapidly in serum‐free media. The concentrations of IGF‐I and IGF‐II in the media were higher in NOR‐P1 than the other cell lines. Cell proliferation in NOR‐P1 cells was enhanced by IGF‐I or IGF‐II treatment more than in MIA‐Paca2 or PK‐1 cells. PPP, LY294002, and PD98059 suppressed proliferation and motility of NOR‐P1 cells and inhibited BrdU uptake, while PPP induced apoptosis. IGF‐IRβ may be a potential therapeutic target to inhibit invasion of pancreatic cancer. J. Cell. Biochem. 113: 2714–2720, 2012. © 2012 Wiley Periodicals, Inc.
Protein arginine methylation of SERBP1 by protein arginine methyltransferase 1 affects cytoplasmic/nuclear distribution
Authors: Lee Yu‐Jen , Hsieh Wan‐Yu , Chen Ling‐Yun , Li Chuan 
Published: March 2012

AbstractProtein arginine methylation regulates a broad array of cellular processes. SERBP1 implicated in tumor progression through its putative involvement in the plaminogen activator protease cascade, is an RNA‐binding protein containing an RG‐rich domain and an RGG box domain that might be methylated by protein arginine N‐methyltransferases (PRMTs). Asymmetric dimethylarginine (aDMA) was detected in SERBP1 and an indirect methyltransferase inhibitor adenosine dialdehyde (AdOx) significantly reduced the methylation signals. Arginines in the middle RG and C‐terminal RGG region of SERBP1 are methylated based on the analyses of different deletion constructs. The predominant type I protein arginine methyltransferase PRMT1 co‐immunoprecipitated with SERBP1 and the level of bound PRMT1 decreased upon the addition of AdOx. Recombinant PRMT1 methylated SERBP1 and knockdown of PRMT1 significantly reduced the aDMA level of SERBP1, indicating that SERBP1 is specifically methylated by PRMT1. Immunofluorescent analyses of endogenous SERBP1 showed predominant cytoplasmic localization of SERBP1. Treatment of AdOx or PRMT1 siRNA increased the nuclear localization of SERBP1. Analyses of different deletions indicated that the middle RG region is important for the nuclear localization while both N‐ and C‐ terminus are required for nuclear export. Low methylation of the C‐terminal RGG region also favors nuclear localization. In conclusion, the RG‐rich and RGG box of SERBP1 is asymmetrically dimethylated by PRMT1 and the modification affects protein interaction and intracellular localization of the protein. These findings provide the basis for dissecting the roles of SERBP1. J. Cell. Biochem. 113: 2721–2728, 2012. © 2012 Wiley Periodicals, Inc.
Short‐term degradation of semiarid grasslands—results from a controlled‐grazing experiment in Northern China
Authors: Wiesmeier Martin , Kreyling Olivia , Steffens Markus , Schoenbach Philipp , Wan Hongwei , Gierus Martin , Taube Friedhelm , Kölbl Angelika , Kögel‐Knabner Ingrid 
Published: March 2012

AbstractThe assessment of grassland degradation due to overgrazing is a global challenge in semiarid environments. In particular, investigations of beginning steppe degradation after a change or intensification of the land use are needed in order to detect and adjust detrimental land‐use management rapidly and thus prevent severe damages in these sensitive ecosystems. A controlled‐grazing experiment was established in Inner Mongolia (China) in 2005 that included ungrazed (UG) and heavily grazed plots with grazing intensities of 4.5 (HG4.5) and 7.5 (HG7.5) sheep per hectare. Several soil and vegetation parameters were investigated at all sites before the start of the experiment. Topsoil samples were analyzed for soil organic C (SOC), total N (Ntot), total S (Stot), and bulk density (BD). As vegetation parameters, aboveground net primary productivity (ANPP), tiller density (TD), and leaf‐area index (LAI) were determined. After 3 y of the grazing experiment, BD increased and SOC, Ntot, Stot, ANPP, and LAI significantly decreased with increasing grazing intensity. These sensitive parameters can be regarded as early‐warning indicators for degradation of semiarid grasslands. Vegetation parameters were, however, more sensitive not only to grazing but also to temporal variation of precipitation between 2006 and 2008. Contrary, soil parameters were primarily affected by grazing and resistant against climatic variations. The assessment of starting conditions in the study area and the application of defined grazing intensities is essential for the investigation of short‐term degradation in semiarid environments.
A new heart: Somatic stem cells and myocardial regeneration
Authors: Mullenix Philip S. , Huddleston Steven J. , Stojadinovic Alexander , Trachiotis Gregory D. , Alexander Edmund P. 
Published: March 2012

AbstractEnd‐stage heart failure is a global scourge. Current therapies merely delay its inexorable progression. Heart transplantation is resource‐intensive and limited by organ availability. Bone marrow‐derived and cardiac‐specific stem cells have demonstrated potential for cardiac regeneration and repair, but the magnitude and durability of these promising findings are inconsistent. The purpose of this review is to (1) describe cells currently being investigated, (2) outline the status of current trials, and (3) discuss key objectives of future research. J. Surg. Oncol. 2012; 105:475–480. © 2012 Wiley Periodicals, Inc.
Comparison of clinical outcomes for patients with clinical N0 and pathologic N2 non‐small cell lung cancer after thoracoscopic lobectomy and open lobectomy: A retrospective analysis of 76 patients
Authors: Li Yun , Wang Jung 
Published: March 2012

AbstractObjectiveTo compare outcomes between thoracoscopic and thoracotomy lobectomy for patients with cN0‐pN2 NSCLC.MethodsOne hundred eighty‐two cN0 patients (including 29 pN2) underwent VATS lobectomy between September 2006 and December 2009, and 204 cN0 patients (including 47 pN2) underwent thoracotomy lobectomy between July 2000 and December 2009. Pre‐ and intraoperative status and postoperative survival between two groups were compared.ResultsThere was no difference in preoperative conditions between two groups except Quantity of smoking. Operation time and blood loss of VATS group was significantly lower than thoracotomy group. Number of dissected lymph node (LN) stations was 3.3 ± 1.1 in VATS group versus 3.3 ± 1.3 in thoracotomy group, and the total number of LN was 12.7 ± 8.9 in VATS group versus 10.5 ± 7.2 in thoracotomy group. One‐ and 3‐year disease‐free survival rate was 82.6% in VATS group versus 72.0% in thoracotomy group, and 49.3% in VATS group versus 51.3% in thoracotomy group (P = 0.996). One‐ and 3‐year survival rate was 84.9% in VATS group versus 71.2% in thoracotomy group, and 64.0% in VATS group versus 42.7% in thoracotomy group (P = 0.121).ConclusionsVATS lobectomy is comparable with thoracotomy in both safety and curability for the treatment of cN0‐pN2 NSCLC, without necessity of conversion to open surgery. J. Surg. Oncol. 2012; 106:431–435. © 2012 Wiley Periodicals, Inc.
Low SPINK5 expression in chronic rhinosinusitis
Authors: Fruth Kai , Goebel Gyula , Koutsimpelas Dimitrios , Gosepath Jan , Schmidtmann Irene , Mann Wolf J. , Brieger Juergen 
Published: March 2012

AbstractObjectives/Hypothesis:Chronic rhinosinusitis (CRS) is a multifactorial disease that probably arises as a result of genetic diversity and environmental factors. SPINK5 is a serine protease inhibitor, which is supposed to be an important regulator of epithelial barrier maintenance. The role of SPINK5 polymorphisms and expression in CRS, especially in individuals with aspirin intolerance, is unclear.Study Design:SPINK5 single‐nucleotide polymorphisms (SNPs) and SPINK5 expression levels were correlated with CRS without (CRSsNP) and with nasal polyps (CRSwNP), aspirin intolerance, asthma, and allergies.Methods:One hundred four nasal tissue samples, 15 from patients with CRSsNP, 59 from patients with CRSwNP, and 30 from healthy controls of the inferior turbinate, were analyzed for their SPINK5 status. Genotypes of four SPINK5 single nucleotide polymorphism (SNPs; G1258A, G2475T, A2915G, and A1103G), as well as SPINK5 mRNA expression levels, were determined by polymerase chain reaction.Results:No correlation between any SPINK5 SNP and CRSsNP, CRSwNP, or allergies and asthma was observed. The heterozygous SNPs G1258A and A1103G were observed more frequently in aspirin‐intolerant patients; the homozygous (A/A) genotype of SNP 1258 and the homozygous (G/G) genotype SNP 1103 were less frequent. There was no correlation between the analyzed SNPs and the level of SPINK5 expression. It was noted that in individuals with CRSwNP, aspirin intolerance, and allergies, SPINK5 expression was lowered.Conclusions:G1258A and A1103G polymorphisms are distinctive for the aspirin intolerance syndrome. Lowered SPINK5 expression might be a contributing factor leading to CRS, and appears to be characteristic for patients suffering from aspirin intolerance and from allergies. Laryngoscope, 2012
Thyroid cancer characteristics in the population surrounding three mile island
Authors: Goyal Neerav , Camacho Fabian , Mangano Joseph , Goldenberg David 
Published: March 2012

AbstractObjectives/Hypothesis:To determine differences in disease characteristics between the thyroid cancer populations in the area around the Three Mile Island (TMI) nuclear power plant and the rest of the state of Pennsylvania.Study Design:Retrospective cross‐sectional study.Methods:Data from the Pennsylvania Cancer Registry from 1985 to 2008 were reviewed and information regarding age at diagnosis, sex, race, residential status, county of residence, thyroid pathology, thyroid surgery, and staging was recorded. Dauphin, Lancaster, and York counties were defined as the TMI area.Results:Records of 26,357 thyroid cancer patients were reviewed, with 2,611 patients within the TMI area. A higher proportion of papillary thyroid cancer (P < .001) and lower proportion of follicular thyroid cancer (P < .001) were noted in the TMI area population. Thyroid cancer cases from the TMI area were found to be more likely to be diagnosed before the age of 65 years (P < .001), be Pennsylvania born (P < .001), be well differentiated (P < .001), be <10 mm in size (P < .001), and be localized without spread (P < .001). Although the TMI area shows a higher incidence of thyroid cancer as compared to the rest of the state, this was not statistically significant.Conclusions:The TMI population showed a higher proportion of papillary thyroid cancer and less aggressive pathology and earlier diagnosis compared to the rest of Pennsylvania. No statistically significant difference in thyroid cancer incidence was noted. Overall, the study does not show a clear link with more advanced thyroid cancer and proximity to the TMI nuclear reactors. Laryngoscope, 2012
Asymptotics of solutions of the heat equation in cones and dihedra
Authors: Kozlov V. A. , Rossmann J. 
Published: March 2012

AbstractThe authors deal with the asymptotics of solutions of the first boundary value problem for the heat equation near vertices of cones or edges. They obtain estimates for the remainder in weighted Lp, q Sobolev spaces. The paper generalizes the main result of Kozlov and Maz’ya 6 to the case \documentclass{article}\usepackage{amssymb}\begin{document}\pagestyle{empty}$p,q\not=2$\end{document} and to edges.
Nitrogen‐Doped Carbon Capsules via Poly(ionic liquid)‐Based Layer‐by‐Layer Assembly
Authors: Zhao Qiang , Fellinger Tim‐Patrick , Antonietti Markus , Yuan Jiayin 
Published: March 2012

AbstractLayer‐by‐layer (LbL) assembly technique is applied for the first time for the preparation of nitrogen‐doped carbon capsules. This approach uses colloid silica as template and two polymeric deposition components, that is, poly(ammonium acrylate) and a poly (ionic liquid) poly(3‐cyanomethyl‐1‐vinylimidazolium bromide), which acts as both the carbon precursor and nitrogen source. Nitrogen‐doped carbon capsules are prepared successfully by polymer wrapping, subsequent carbonization and template removal. The as‐synthesized carbon capsules contain ≈7 wt% of nitrogen and have a structured specific surface area of 423 m2 g−1. Their application as supercapacitor has been briefly introduced. This work proves that LbL assembly methodology is available for preparing carbon structures of complex morphology.
Comparison of reconstruction plate and double flap for reconstruction of an extensive mandibular defect
Authors: Miyamoto Shimpei , Sakuraba Minoru , Nagamatsu Shogo , Kamizono Kenichi , Hayashi Ryuichi 
Published: March 2012

AbstractFunctional reconstruction of the anterior mandibular defect in combination with a significant glossectomy is a challenging problem for reconstructive micro‐surgeons. In this retrospective study, clinical results were compared between mandibular reconstruction plate (MRP) procedures and double flap transfers. The subjects were 23 patients who underwent immediate reconstruction, after an anterior segmental mandibulectomy in combination with a significant glossectomy, from 1993 to 2009. The patients were divided into two groups based on the reconstructive methods used: MRP and soft tissue free flap transfer (MRP group: 12 patients) or double free flap transfer (double flap group: 11 patients). Operative stress, postoperative complications and oral intake ability were compared between the groups. The rate of recipient‐site complication in the double flap group tended to be lower than that in the MRP group. The most frequent complications in the MRP group included infection and orocutaneous fistula. Operative stresses (operation time and blood loss) were significantly less in the MRP group than in the double flap group. Overall, 19 patients (82.6%) were able to tolerate an oral diet without the need for tube feeding. This study demonstrates that laryngeal preservation is possible in more than 80% of patients even after such an extensive ablation. Double flap transfer provides a more stable wound closure than MRP and should be the preferred reconstructive procedure if the patients can tolerate the associated operative stresses. © 2012 Wiley Periodicals, Inc. Microsurgery, 2012.
3D numerical modelling of bit–rock fracture mechanisms in percussive drilling with a multiple‐button bit
Authors: Saksala Timo 
Published: March 2012

SUMMARYThis paper considers numerical modelling of rock fracture induced by dynamic bit–rock interaction in percussive drilling. The work presented here extends the author's earlier research on the topic from the axisymmetric case to 3D case. The numerical method for modelling rock fracture includes a constitutive model for rock and a contact mechanics‐based technique to simulate the bit–rock interaction. The constitutive model is based on a combination of the recent viscoplastic consistency model, the isotropic damage concept and a parabolic compression cap. This model is improved here from its earlier state by calibrating the softening laws using fracture energies GIc and GIIc in tension and compression, respectively. Moreover, the viscosity modulus in tension is calibrated based on the dynamic Brazilian disc test. With these enhancements, the developed method is applied to 3D case of the bit–rock interaction problem assuming one symmetry plane. Single impact with single and multiple‐button bits is simulated. In the latter case, an initial borehole is modelled in order to simulate the usual in‐situ drilling conditions. The different failure types observed in the experiments as well as the interaction between the buttons resulting in chipping are realistically captured in the simulations. Copyright © 2012 John Wiley & Sons, Ltd.
Desmopressin orally disintegrating tablet effectively reduces nocturia: Results of a randomized, double‐blind, placebo‐controlled trial
Authors: Weiss Jeffrey P. , Zinner Norman R. , Klein Bjarke M. , Nørgaard Jens Peter 
Published: March 2012

AbstractAimsThe primary objective was to investigate the efficacy of desmopressin orally disintegrating tablet versus placebo in patients with nocturia. Pharmacodynamics, safety and patient‐reported quality of life (QoL) outcomes were also evaluated. One of several benefits of the new formulation is increased bioavailability. Exploring lower doses allows for a better evaluation of therapeutic effect versus tolerability.MethodsThis was a 4‐week, randomized, double‐blind study comparing 10, 25, 50, or 100 µg desmopressin versus placebo in adults with defined nocturia.ResultsThe intent to treat population comprised 757 patients experiencing ∼3 voids/night and a high prevalence of nocturnal polyuria (∼90%). Increasing doses of desmopressin were associated with decreasing numbers of nocturnal voids and voided volume, greater proportions of subjects with >33% reduction in nocturnal voids, and increased duration of first sleep period. The lowest dose reaching statistical significance (P < 0.05 vs. placebo) varied by endpoint. Improvements were clinically meaningful, meaning that patients actually had fewer nightly voids. Post hoc analyses by gender suggested a lower minimum effective dose for women. Desmopressin was generally well tolerated. Reductions in serum sodium to <125 mmol/L in six women (taking >25 µg desmopressin) and two men (aged 67 and 82) taking 100 µg, support lower and gender‐specific dosing to reduce the small but clinically significant risk of hyponatraemia. Each void reduced/hour of sleep gained was associated with significant improvements in QoL.ConclusionsDesmopressin orally disintegrating tablet is an effective and well‐tolerated treatment for patients with nocturia. Further exploration of the lower dose range is warranted. Neurourol. Urodynam. 31:441–447, 2012. © 2012 Wiley Periodicals, Inc.
Approaching an organic semimetal: Electron pockets at the Fermi level for a p‐benzoquinonemonoimine zwitterion
Authors: Rosa Luis G. , Velev Julian , Zhang Zhengzheng , Alvira Jose , Vega Omar , Diaz Gerson , Routaboul Lucie , Braunstein Pierre , Doudin Bernard , Losovyj Yaroslav B. , Dowben Peter A. 
Published: March 2012

AbstractThere is compelling evidence of electron pockets, at the Fermi level, in the band structure for an organic zwitterion molecule of the p‐benzoquinonemonoimine type. The electronic structure of the zwitterion molecular film has a definite, although small, density of states evident at the Fermi level as well as a nonzero inner potential and thus is very different from a true insulator. In spite of a small Brillouin zone, significant band width is observed in the intermolecular band dispersion. The results demonstrate that Bloch's theorem applies to the wave vector dependence of the electronic band structure formed from the molecular orbitals of adjacent molecules in a molecular thin film of a p‐benzoquinonemonoimine type zwitterion.magnified image
Preface: Indium nitride and related alloys
Authors: Ruterana Pierre 
Published: March 2012

AbstractThe present proceedings, which comprise 25 articles in a joint issue of physica status solidi (a) and (c), contain recent advances in the properties and applications of indium based nitride alloys and heterostructures. The manuscripts have been submitted to the Symposium H of the 2011 European Materials Research Society (E‐MRS) Spring l Meeting, held in Nice on 9–13th May. The goal of the symposium was to highlight the developments in growth and characterization of InN material as well as in related alloys and heterostructures for device applications.Research on indium nitride and related alloys received a significant boost in Europe through the Marie Curie Initial Training Network (ITN) called “RAINBOW”, where 19 Ph.D. students and 8 post‐docs are trained in materials growth, characterization and related physics (EC funding 4.8 M€ for 2008–2012). This three‐days symposium was organized in the scope of the RAINBOW ITN. Such an organization proved to be highly successful: from the initially registered 80 contributors, the sessions were overcrowded all along with participants attracted from other symposia.The symposium discussed over 70 contributions, metal organic vapour phase epitaxy and molecular beam epitaxy of InN were covered, and special attention was given to the electronic, optical and structural quality of thin films. 16 invited speakers provided the current status of the research in this exciting field. In addition to invited papers, 45 oral presentations contributed to the sessions ranging from structural and optical properties to devices. Moreover, a poster session, which presented 21 reports, provided an important forum for detailed discussions. From the papers that were submitted for publication, 18 were highly rated, each by two reviewers and are published in physica status solidi (a). This issue is organised in three sections which reflect the topical sessions of the symposium. The first deals with the growth, optical and structural properties of indium nitride and related alloys, the second reports on the current theoretical investigations and the third section is devoted to the transport and point defects (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)