bluuebunny/crossref_metadata_2025_split huggingface dataset:
Are outcomes of adjuvant vaginal vault brachytherapy in endometrial cancer related to the way it is delivered?
Authors: Alzouebi M. , Pledge S.D. , Martin J.E. 
Published: December 2011

AbstractAims:Endometrial cancer is the commonest malignancy of the female genital tract. Surgery forms the cornerstone of treatment with adjuvant therapy proven to reduce local recurrence without demonstrating a clear survival benefit. The selection of adjuvant therapy is becoming increasingly more complex. The aim of this study was to investigate current adjuvant practices by reviewing outcomes of patients with endometrial cancer treated with intracavitary vaginal brachytherapy (VB).Materials & Methods:A retrospective analysis was carried out of all women with Stage II endometrial endometroid adenocarcinoma treated at Weston Park Hospital, Sheffield with adjuvant VB from 2003–2006. The data collected and analysed included histology, stage and grade of disease, radiotherapy treatment–related parameters, morbidity, recurrence rates and survival rates. Kaplan-Meier was used to analyse recurrence-free and overall survival rates. Wilson’s score was used to determine statistical significance of outcomes. Attention was focused on the method of treatment delivery, and this was compared with available literature.Results:In total, 33 patients were identified. All patients were treated with LDR 48 Gy prescribed to the surface of the applicator. Median follow-up was 36 months. Vaginal, pelvic and distant relapse rates were 9%, 15% and 18%, respectively. Recurrence-free and overall survival rates were 78.8% and 84.8%, respectively. Six of the seven patients (86%) who recurred developed distant metastases, not influenced by VB. No severe (Grade 3 or 4 toxicity) was recorded. When vaginal relapse rates were compared to published trials based on technique used, no statistically significant difference was demonstrated.Conclusion:Rates of vaginal relapses were comparable to the available literature suggesting current VB practice is an effective adjuvant local treatment. The study highlights the importance of surveillance and patient education regarding toxicity and its prevention with particular attention drawn to vaginal stenosis.
Conservation of Billbergia zebrina genetic resources: AFLP polymorphism of in vitro regenerated genotypes
Authors: Dal Vesco Lirio L. , Stefenon Valdir M. , Welter Leocir J. , Steiner Neusa , Guerra Miguel P. 
Published: December 2011

Biotechnological techniques comprise useful tools for the conservation of endangered plant genetic resources. In the present work, polymorphism and usefulness of amplified fragment length polymorphism (AFLP) markers in assessing the genetic diversity in populations of Billbergia zebrina were investigated in nodular cultures and adult plants of the species. AFLP markers revealed moderate-to-high genetic diversity based on the estimations of Nei's gene diversity (mean He = 0.28), Shannon index of diversity (mean HS = 0.48) and the number of polymorphic fragments (mean of 56.17 polymorphic fragments over six primer pairs). In comparison to published studies of population genetics performed in other bromeliad species, the present study suggests that natural populations of B. zebrina likely maintain high levels of genetic diversity, an important feature towards conservation of plant genetic resources. The results obtained reveal that AFLP markers comprise a powerful tool in order to assess the levels of genetic diversity in natural populations of this endangered species. Integrating AFLP markers with in vitro propagation techniques is understood as an adequate strategy for conservation programmes of this species.
Modularization for the Cell Ontology
Authors: Torniai Carlo , Diehl Alexander , Mungall Christopher , Haendel Melissa , Ireland Amelia , Manzoor Shahid , Meehan Terry , Osumi-Sutherland David 
Published: December 2011

AbstractOne of the premises of the OBO Foundry is that development of an orthogonal set of ontologies will increase domain expert contributions and logical interoperability, and decrease maintenance workload. For these reasons, the Cell Ontology (CL) is being re-engineered. This process requires the extraction of sub-modules from existing OBO ontologies, which presents a number of practical engineering challenges. These extracted modules may be intended to cover a narrow or a broad set of species. In addition, applications and resources that make use of the Cell Ontology have particular modularization requirements, such as the ability to extract custom subsets or unions of the Cell Ontology with other OBO ontologies. These extracted modules may be intended to cover a narrow or a broad set of species, which presents unique complications.We discuss some of these requirements, and present our progress towards a customizable simple-to-use modularization tool that leverages existing OWL-based tools and opens up their use for the CL and other ontologies.
A molten globule-to-ordered structure transition of Drosophila melanogaster crammer is required for its ability to inhibit cathepsin
Authors: Tseng Tien-Sheng , Cheng Chao-Sheng , Chen Dian-Jiun , Shih Min-Fang , Liu Yu-Nan , Hsu Shang-Te Danny , Lyu Ping-Chiang 
Published: December 2011

Drosophila melanogaster crammer is a novel cathepsin inhibitor that is involved in LTM (long-term memory) formation. The mechanism by which the inhibitory activity is regulated remains unclear. In the present paper we have shown that the oligomeric state of crammer is pH dependent. At neutral pH, crammer is predominantly dimeric in vitro as a result of disulfide bond formation, and is monomeric at acidic pH. Our inhibition assay shows that monomeric crammer, not disulfide-bonded dimer, is a strong competitive inhibitor of cathepsin L. Crammer is a monomeric molten globule in acidic solution, a condition that is similar to the environment in the lysosome where crammer is probably located. Upon binding to cathepsin L, however, crammer undergoes a molten globule-to-ordered structural transition. Using high-resolution NMR spectroscopy, we have shown that a cysteine-to-serine point mutation at position 72 (C72S) renders crammer monomeric at pH 6.0 and that the structure of the C72S variant highly resembles that of wild-type crammer in complex with cathepsin L at pH 4.0. We have determined the first solution structure of propeptide-like protease inhibitor in its active form and examined in detail using a variety of spectroscopic methods the folding properties of crammer in order to delineate its biomolecular recognition of cathepsin.
Daily physical activity and sports participation among children from ethnic minorities in Denmark
Authors: Nielsen Glen , Hermansen Bianca , Bugge Anna , Dencker Magnus , Andersen Lars Bo 
Published: December 2011

AbstractThe purpose of this study was to investigate whether Danish children from immigrant backgrounds are less physically active than children from the ethnic majority, and to investigate the possible reasons for any differences found. Accelerometer measures of physical activity as well as questionnaire data about organised sports, family demography, resources and values were collected from 594 children of whom 67 had other ethnic background than Danish. Data were collected when the children were 6–7 years old and again later when the children were 9–10 years old. It was found that children from immigrant backgrounds were not less physically active than other children when their amounts of daily physical activity were measured by direct objective measures, despite their participation rate in organised sports being much lower. Using multiple logistic regression modelling, this study showed that lack of parental experience with organised sports and lack of economic/material resources explained much of the difference in sports participation. Children of immigrant background had significant lower participation in club sports but this did not affect their overall physical activity level.
The relationship between mental toughness and dispositional flow
Authors: Crust Lee , Swann Christian 
Published: December 2011

AbstractThis article tested the relationship between mental toughness (MT) and dispositional flow. A sample of 135 athletes (M age=20.81 years, SD=2.76), derived from University sports teams and local sports clubs, gave informed consent before completing questionnaires to assess MT and dispositional flow. Pearson correlations revealed a significant and positive relationship between total MT and global flow scores (r=0.65, p <0.001). Correlations between total MT and dispositional flow subscales were all found to be significant and positive, ranging from 0.26 to 0.68. Correlations between global flow and MT subscales were also all significant and positive, ranging between 0.43 and 0.65. Linear regression analyses found MT subscales to account for 45% of the variance in disposition flow, and flow subscales to account for 50% of the variance in MT. These results suggest a reciprocal relationship between MT and flow.
The relationship between physical fitness and game behaviours in rugby union players
Authors: Smart Daniel , Hopkins Will G. , Quarrie Kenneth L. , Gill Nicholas 
Published: December 2011

AbstractThe physical preparation of team sport athletes should reflect the degree to which each component of fitness is relied upon in competition. The aim of the study was therefore to establish the relationship between fitness‐test data and game behaviours known or thought to be important for successful play in rugby union matches. Fitness‐test measures from 510 players were analysed with game statistics, from 296 games within the 2007 and 2008 calendar years. Sprint times over 10, 20 and 30 m had moderate to small negative correlations (r) with line breaks (~0.26), metres advanced (~0.22), tackle breaks (~0.16) and tries scored (~0.15). The average time of 12 repeated sprints and percentage body fat in the forwards, and repeated sprint fatigue in the backs had moderate to small correlations with a measure of activity rate on and around the ball (−0.38, –0.17 and –0.17, respectively). These low correlations are partly due to uniformly high physical fitness as a result of selection pressures at the elite level and leave room for the identification of other key predictors. Nonetheless, physical conditioning programmes should be adapted to reflect the importance of speed, repeated sprint ability and body composition in the performance of key game behaviours during competition.
Interrelationships between different loads in resisted sprints, half‐squat 1 RM and kinematic variables in trained athletes
Authors: Martínez‐Valencia María Asunción , González‐Ravé José M. , Santos‐García Daniel Juárez , Alcaraz Ramón Pedro E. , Navarro‐Valdivielso Fernando 
Published: December 2011

AbstractResisted sprint running is a common training method for improving sprint‐specific strength. It is well‐known that an athlete's time to complete a sled‐towing sprint increases linearly with increasing sled load. However, to our knowledge, the relationship between the maximum load in sled‐towing sprint and the sprint time is unknown, The main purpose of this research was to analyze the relationship between the maximum load in sled‐towing sprint, half‐squat maximal dynamic strength and the velocity in the acceleration phase in 20‐m sprint. A second aim was to compare sprint performance when athletes ran under different conditions: un‐resisted and towing sleds. Twenty‐one participants (17.86±2.27 years; 1.77±0.06 m and 69.24±7.20 kg) completed a one repetition maximum test (1 RM) from a half‐squat position (159.68±22.61 kg) and a series of sled‐towing sprints with loads of 0, 5, 10, 15, 20, 25, 30% body mass (Bm) and the maximum resisted sprint load. No significant correlation (P<0.05) was found between half‐squat 1 RM and the sprint time in different loaded conditions. Conversely, significant correlations (P<0.05) were found between maximum load in resisted sprint and sprint time (20‐m sprint time, r=−0.71; 5% Bm, r=−0.73; 10% Bm, r=−0.53; 15% Bm, r=−0.55; 20% Bm, r=−0.65; 25% Bm, r=−0.44; 30% Bm, r=−0.63; MaxLoad, r= 0.93). The sprinting velocity significantly decreased by 4–22% with all load increases. Stride length (SL) also decreased (17%) significantly across all resisted conditions. In addition, there were significant differences in stride frequency (SF) with loads over 15% Bm. It could be concluded thatthe knowledge of the individual maximal load in resisted sprint and the effects on the sprinting kinematic with different loads, could be interesting to determinate the optimal load to improve the acceleration phase at sprint running.
Fluid simulation of the phase-shift effect in hydrogen capacitively coupled plasmas: I. Transient behaviour of electrodynamics and power deposition
Authors: Zhang Yu-Ru , Xu Xiang , Bogaerts Annemie , Wang You-Nian 
Published: December 2011

Abstract
A two-dimensional self-consistent fluid model coupled with the full set of Maxwell equations is established to investigate the phase-shift effect on the transient behaviour of electrodynamics and power deposition in a hydrogen capacitively coupled plasma. The effect has been examined at 13.56 MHz and 100 MHz, respectively, because of the different phase-shift modulation when the electromagnetic effects are dominant. The results indicate that the spatiotemporal distributions of the plasma characteristics obtained for various phase-shift cases are obviously different both in shape and especially in absolute values. Indeed, when the phase difference varies from 0 to π, there is an increase in the electron flux, thus the power deposition becomes more pronounced. At the frequency of 13.56 MHz, the axial electron flux in the bulk plasma becomes uniform along the z-axis, and the radial electron flux exhibits two peaks within one period at the reverse-phase case, whereas the oscillation is less pronounced at the in-phase case. Furthermore, in the very high frequency discharge, the radial electron flux is alternately positive and negative with four peaks during one period, and the ionization mainly occurs in the sheath region, due to the prominent power deposition there at a phase difference equal to π.
Opening a larger window onto forest elephant ecology
Authors: Wrege Peter H. , Rowland Elizabeth D. , Bout Nicolas , Doukaga Modeste 
Published: December 2011

AbstractAfrican forest elephants (Loxodonta cyclotis) can be observed reliably in forest clearings but are difficult to observe directly in their otherwise forested habitat. To obtain data on population demography and activity cycles, conservation programmes commit considerable human and financial resources to observations at clearings. However, individual elephants spend only a tiny proportion of their annual activity budget in a particular clearing, and there may be demographic differences between daytime and nighttime populations. Using acoustic monitoring tools to assess elephant numbers continuously for long time periods at multiple clearings, we show that daytime‐only observations are likely to be biased. In this study, (i) 79% of all elephant visitation occurred at night, (ii) sometimes large changes in nocturnal elephant numbers occurred without proportional changes in daytime elephant numbers, and (iii) there were indications that a different demographic of the population visits clearings at night compared with the day. These results suggest that acoustic monitoring should be incorporated into forest elephant monitoring programmes to augment direct observation and that more study is needed to explicitly identify any biases inherent in daytime‐only observation.
Current treatment of severe asthma
Authors: Hashimoto S. , Bel E. H. 
Published: December 2011

SummarySevere asthma is considered a heterogeneous disease in which a variety of clinical, physiological and inflammatory markers determine disease severity. Pivotal studies in the last 5 years have led to substantial progress in many areas, ranging from a more accurate definition of truly severe, refractory asthma, to classification of the disease into distinct clinical phenotypes, and introduction of new therapies. This review focuses on three common clinical phenotypes of severe asthma in adults (early onset severe allergic asthma, late onset non‐atopic eosinophilic asthma, late onset non‐eosinophilic asthma with obesity), and provides an overview of recent developments regarding treatment options that are best suited for each of these phenotypes.
Glucocorticoid replacement therapy and fibrinolysis in patients with hypopituitarism
Authors: Peacey Steven R. , Wright Dianne , Aye Mo , Moisey Robert 
Published: December 2011

SummaryBackground  Hypopituitarism is associated with increased cardiovascular mortality, and it has been suggested that unphysiological glucocorticoid replacement regimens might contribute to this risk. Traditional glucocorticoid replacement regimens have often led to excessive serum cortisol levels. The hypercortisolaemia of Cushing’s syndrome is associated with an increased risk of thromboembolism.Objective  To examine whether short‐term higher‐dose hydrocortisone replacement regimens adversely affect the fibrinolytic system.Design  Crossover study comparing tailored low‐dose (LD) glucocorticoid regimen (mean, 17·5 mg hydrocortisone daily), with a traditional high‐dose (HD, 30‐mg hydrocortisone daily) regimen for 2 weeks.Patients  Ten patients with hypopituitarism and ACTH deficiency – median (range) age, 59 (41–75) years – and 10 age‐ and sex‐matched controls. Nine patients had growth hormone deficiency (five replaced), nine patients had TSH deficiency (nine replaced), eight had gonadotrophin deficiency (five replaced). During the study, other pituitary hormone replacement therapy remained unchanged. Patients with acromegaly and Cushing’s syndrome were excluded.Measurements  Hourly serum cortisol for 11 h, plasminogen activator inhibitor‐1 (PAI‐1), tissue plasminogen activator (tPA) and fibrinogen levels after 2 weeks of treatment with both LD and HD regimens.Results  No overall significant differences were found between the three groups using the Kruskal–Wallis test: PAI‐1: [median (range)] HD, 25 (5–53) ng/ml; LD, 21 (4–56) ng/ml; controls, 27 (8–51); P = 0·3; tPA: HD, 10 (5–15) ng/ml; LD, 10 (4–13) ng/ml; controls 10 (3–13); P = 0·46; and fibrinogen: HD, 2·5 (1·8–3·5) g/l; LD, 3·0 (2·3–4·4) g/l; controls, 2·6 (1·6–3·2): P = 0·97 In addition, no significant differences between HD and LD using Wilcoxon’s paired test; PAI‐1 (P = 0·91), tPAag (P = 0·47) and fibrinogen (P = 0·09).Conclusions  An increased dose of hydrocortisone for 2 weeks creates excessive glucocorticoid exposure, but does not significantly affect fibrinolytic‐coagulation parameters.
Possibilities of increasing the residual water‐soluble carbohydrate concentration and aerobic stability of low dry‐matter perennial ryegrass silage through additive and cultivar use
Authors: Conaghan P. , O’Kiely P. , O’Mara F. P. 
Published: December 2011

AbstractIncreasing the residual water‐soluble carbohydrate (WSC) concentration in silage may improve the nutritional value but impair aerobic stability. Our aim was to determine whether the residual WSC concentration and aerobic stability of low dry‐matter (<135 g kg−1) perennial ryegrass silage could be manipulated through the judicious use of additive and cultivar. Seven additive treatments, including three innovative treatments, were compared across four consecutive harvests of the cultivars AberDart (bred to accumulate high concentrations of herbage WSC) and Fennema (control). The standard of fermentation of silage ensiled without additive (untreated) ranged from very bad to excellent. Application of ammonium tetraformate, at 3 and 6 L t−1, or homofermentative lactic acid bacteria (LAB) alone had an inconsistent effect on the fermentation and aerobic stability, and negligible effect on residual WSC concentration. A mixture ofLactobacillus buchneriand homofermentative LAB was not an effective silage additive, producing generally poorly fermented silage. An antimicrobial mixture of sodium benzoate, sodium propionate, sodium nitrite and hexamethylenetetramine, applied at 2·5 and 5 L t−1, frequently improved the standard of fermentation, but the effects were subject to the application rate. The high application rate was the most effective additive evaluated at improving the fermentation and increasing residual WSC concentration and consistently produced silage of excellent standard of fermentation. However, the antimicrobial mixture was not effective at protecting against aerobic instability. The effects of additive treatment were largely inconsistent across cultivars. Overall, AberDart had a negligible effect on the silage fermentation, residual WSC concentration and aerobic stability compared with Fennema.
Impact of deferred grazing and fertilizer on plant population density, ground cover and soil moisture of native pastures in steep hill country of southern Australia
Authors: Nie Z. N. , Zollinger R. P. 
Published: December 2011

AbstractThe impact of deferred grazing (no defoliation of pastures for a period generally from spring to autumn) and fertilizer application on plant population density, ground cover and soil moisture in a hill pasture (annual grass dominated, with Australian native grasses being the major perennial species) were studied in a large‐scale field experiment from 2002 to 2006 in southern Australia. Three deferred grazing strategies were used: short‐term deferred grazing (no defoliation between October and January each year), long‐term deferred grazing (no defoliation from October to the autumn break, that is the first significant rainfall event of the winter growing season) and optimized deferred grazing (withholding time from grazing depends on morphological development of the plants). These treatments were applied with two fertilizer levels (nil fertilizer and 50 kg P ha−1 plus lime) and two additional treatments [continuous grazing (control) and no grazing for year 1]. Deferred grazing increased (P < 0·05) perennial grass tiller density compared with the control. On average, the tiller density of the three deferred grazing treatments was 27–88% higher than the control. There was a negative (P < 0·01) relationship between perennial and annual grass tiller density. Fertilizer application increased (P < 0·05) legume plant density. The densities of annual grasses, legumes, onion grass (Romulea rosea) and broadleaf weeds varied between years, but perennial grass density and moss cover did not. The ground cover of the deferred grazing treatments in autumn was on average 27% higher than the control. Soil moisture differed between treatments at 15–30 cm depth, but not at 0–15 depth over autumn and winter. The results imply that deferred grazing can be an effective tool for rejuvenating degraded native pastures through increases in native grass tiller density and population and through improving farm productivity and sustainability.