bluuebunny/crossref_metadata_2025_split huggingface dataset:
The hierarchical importance of patent's characteristics to licensing: An analysis through Random Forest
Authors: Reis Alexânder Araújo , Leite Rafael Ângelo Santos , Walter Cicero Eduardo , Reis Igor Bezerra , Gonçalves Ramiro , Martins José , Branco Frederico , Au‐Yong‐Oliveira Manuel 
Published: June 2024

AbstractThe purpose of this study is to ascertain the hierarchical importance of a patent's characteristics to licensing. This research has a causal‐exploratory purpose, in that it sought to establish relationships between variables. This research aims to identify which characteristics are influential in the licensing of Brazilian academic patents in the biotechnology and pharmaceutical technology fields, based on the mining of data contained in licensed and unlicensed patent documents. Which characteristics of Brazilian academic patents are most influential in their licensing potential? An analysis through Random Forest was performed. To the best of our knowledge, there are no studies in Brazil using machine learning to identify which characteristics are influential in licensing a particular academic patent, especially given the difficulty of gathering this information. We found that regardless of the measure used, the three most critical licensing characteristics for the Biotechnology and Pharmaceutical patents analysed are Patent Scope, Life Cycle, and Claims. At the same time, the least important is the Patent Cooperation Treaty. The relevance of this research is based on the fact that after identifying which intrinsic characteristics influence the final value and licensing probabilities of a given patent, it will be possible to develop mathematical models that provide accurate information for establishing technology transfer agreements. In practical terms, the results suggest that greater patent versatility, combined with lifecycle management and a technical effort to build strong claims, increases the licensing potential of academic biopharmaceutical patents.
Views of Mental Health Nurses on Responding to Clinical Aggression on General Wards
Authors: Olasoji Michael , Henderson Kathryn , Hopkins Liza , Keppich‐Arnold Sandra , Joseph Bindu 
Published: June 2024

ABSTRACTThe overall aim of this study was to explore the views of mental health nurses (MHNs) about their experience of responding to workplace violence (WPV) and aggression (code greys and blacks) within acute general wards. WPV continues to pose a significant source of challenge within healthcare settings despite several initiatives that have been trialled over the years. It has the potential to impact patient care and overall staff health and well‐being. Nurses play a significant role in the management of WPV; hence, it is important to understand how they respond to and manage incidents of WPV. The study design was an explorative descriptive qualitative design. Data were collected through semi‐structured interviews involving n = 10 MHNs working within a mental health consultation liaison team that responds to incidents of violence and aggression (codes grey and black) in acute general wards. Participants were recruited using a convenient sampling method. Data were analysed using thematic analysis. The findings of this study highlighted the following themes: (1) Patient care (subthemes: patient frustration with hospital processes, lack of time and resources); (2) Staffing skills and confidence (subthemes: lack of adequate training, inability to intervene early and communication skills); (3) Understanding patient behaviours (subthemes: it's a psychiatry problem and zero tolerance approach). The management of WPV within acute hospital settings needs to be given due attention by healthcare services. Although a number of organisations are developing measures and guidelines to manage WPV, there is still a gap in the ability of general nursing staff to properly manage such incidences. Proper attention needs to be taken to the training of staff.
Design and Analysis of Bistable Shells With Steered Fiber Paths for Deployable Space Structural Applications
Authors: Hasanyan Armanj D. , Moriel Cesar , Lee Andrew J. , Scalice Niccoli 
Published: June 2024

Abstract
This paper investigates the bistable behavior of shell structures made of fiber reinforced composites with steered fiber paths. The additional freedom provided by varying the spatial orientation of the fibers is shown to allow for the ability to guide the second stable state of the shell. To demonstrate, the analysis is conducted on a tape spring shell structure. If the material layup in the composite tape spring is uniformly antisymmetric throughout the entire structure, it yields a second stable configuration, which is a tight coil. This study shows that by steering the fiber paths, the antisymmetric layup can be locally directed, thus localizing the bistability. Consequently, the overall shape and configuration of the shell in the second state can be controlled through the fiber orientation function in each ply. To demonstrate, the bistable behavior of the tape spring shell structure is investigated both analytically and numerically through finite element approach.
A Parametric Dynamic Mode Decomposition for Reduced-Order Modeling of Highly Flexible Aircraft
Authors: He Tianyi , Su Weihua 
Published: June 2024

Abstract
This paper introduces a parametric Dynamic Mode Decomposition (p-DMD) modeling approach for the nonlinear aeroelasticity of highly flexible aircraft. The method derives a linear parameter-varying reduced-order model (LPV-ROM) directly from data snapshots, which are obtained by feeding excitation input and integrating governing equations. The proposed p-DMD method is capable of addressing varying flight conditions and overcoming the limitations of the traditional DMD method around equilibrium/trim flight. We demonstrate, in this paper, the p-DMD can effectively capture nonlinear aeroelasticity and flight dynamic responses in both time and frequency domains, as demonstrated through comparisons with linearization-based parametric modeling in numerical studies of a highly flexible aircraft at frozen and varying angles of attack.
Short-chain fatty acids abrogate Japanese encephalitis virus-induced inflammation in microglial cells via miR-200a-3p/ZBTB20/IKβα axis
Authors: Majumdar Atreye , Siva Venkatesh Indira Priya , Swarup Vivek , Basu Anirban 
Published: June 2024

ABSTRACT

Japanese encephalitis virus (JEV), a member of the Flaviviridae family, is a leading cause of viral encephalitis in humans. Survivors of this infection often develop lifelong neurological sequelae. Short-chain fatty acids (SCFAs) produced in the gut are vital mediators of the gut-brain axis. We aimed to study microRNA-based mechanisms of SCFAs in an
in vitro
model of JEV infection. N9 microglial cells were pretreated with SCFA cocktail before JEV infection. Cytokine bead analysis, immunoblotting, and PCR were performed to analyze relevant inflammatory markers. microRNA sequencing was performed using Illumina Hiseq, and bioinformatics tools were used for differentially expressed (DE) miRNAs and weighted gene co-expression network analysis (WGCNA). microRNA mimic/inhibitor experiments and luciferase assay were performed to study miRNA-target interaction. A significant reduction in monocyte chemoattractant protein (MCP1) and tumor necrosis factor alpha (TNFα) along with reduced expression of phospho-nuclear factor kappa B (phospho-NF-κB) was observed in SCFA conditions. Significant attenuation of histone deacetylase activity and protein expression was recorded. miRNA sequencing revealed 160 DE miRNAs in SCFA + JEV-treated cells at 6 h post-infection. WGCNA revealed miR-200a-3p, a hub miRNA significantly upregulated in SCFA conditions. Transcription factor ZBTB20 was bioinformatically predicted and validated as a gene target for miR-200a-3p. Further miRNA mimic/inhibitor assay demonstrated that miR-200-3p regulated ZBTB20 along with Iκβα that possibly dampened NF-κB signal activation downstream.

IMPORTANCE

The gut-brain axis plays a pivotal role in the physiological state of an organism. Gut microbiota-derived metabolites are known to play a role in brain disorders including neuroviral infections. Short-chain fatty acids (SCFAs) appear to quench inflammatory markers in Japanese encephalitis virus-infected microglial cells
in vitro
. Mechanistically, we demonstrate the interaction between miR-200a-3p and ZBTB20 in regulating the canonical nuclear factor kappa B (NF-κB) signaling pathway via transcriptional regulation of Iκβα. Findings of this study pave the way to a better understanding of SCFA mechanisms that can be used to develop strategies against viral neuroinflammation.
Effects of an inoculant containing Lentilactobacillus hilgardii (CNCM-I-4785) on the microbiome, fermentation, and aerobic stability of corn silage stored for long term at different incubation temperatures
Authors: Drouin P. , da Silva É.B. , Scuderi R.A. , Tremblay J. , Castex M. , Apper E. 
Published: June 2024

Whole-plant corn untreated (Control) or treated with an inoculant ( Lentilactobacillus buchneri, Lentilactobacillus hilgardii, and Pediococcus pentosaceus, INO) was ensiled for 210 days in mini-silos at constant (MS-C) or variable temperature (MS-V) or for 220 days in bunkers. Bunker samples were collected at 50 (D50) and 150 (D150) cm below silo surface. Samples from MS-V and MS-C had similar pH and concentrations of lactic acid, propionic acid, 1,2-propanediol, and ethanol, but different acetic acid content ( P = 0.009). Additionally, MS-V exhibited greater bacterial ( P = 0.002) and fungal ( P = 0.011) richness than MS-C. Inoculation decreased ( P < 0.001) lactic acid levels while increasing ( P < 0.05) acetic acid, 1,2-propanediol, and fungal richness in both mini-silos and bunker. In bunkers, samples collected from D50 had a lower aerobic stability ( P < 0.05) than D150, but inoculation increased ( P < 0.05) aerobic stability compared to Control, regardless of sampling depth. The storage temperature of mini-silos did not markedly impact the fermentation profile or fungal community. Overall, inoculation increased acetic acid production and fungal diversity in mini-silos, regardless of the storage temperature, and in bunkers, irrespective of sampling the depth, improving the aerobic stability of D50 and D150 bunker silages after long-term ensiling.
Intrapartum maternal glycaemic control for the prevention of neonatal hypoglycaemia: a systematic review and meta-analysis
Authors: Ulyatt Caitlyn M. , Roberts Lily F. , Crowther Caroline A. , Harding Jane E. , Lin Luling 
Published: June 2024

Abstract
Background
Neonatal hypoglycaemia is the most common metabolic disorder in infants, and may be influenced by maternal glycaemic control. This systematic review evaluated the effect of intrapartum maternal glycaemic control on neonatal hypoglycaemia.

Methods
We included randomised controlled trials (RCTs), quasi-RCTs, non-randomised studies of interventions, and cohort or case-control studies that examined interventions affecting intrapartum maternal glycaemic control compared to no or less stringent control. We searched four databases and three trial registries to November 2023. Quality assessments used Cochrane Risk of Bias 1 or the Effective Public Health Practice Project Quality Assessment Tool. Certainty of evidence was assessed using the Grading of Recommendations, Assessment, Development and Evaluation (GRADE). Meta-analysis was performed using random-effects models analysed separately for women with or without diabetes. The review was registered prospectively on PROSPERO (CRD42022364876).

Results
We included 46 studies of women with diabetes and five studies of women without diabetes: one RCT, 32 cohort and 18 case-control studies (11,273 participants). For women with diabetes, the RCT showed little to no difference in the incidence of neonatal hypoglycaemia between tight versus less tight intrapartum glycaemic control groups (76 infants, RR 1.00 (0.45, 2.24), p = 1.00, low certainty evidence). However, 11 cohort studies showed tight intrapartum glycaemic control may reduce neonatal hypoglycaemia (6,152 infants, OR 0.44 (0.31, 0.63), p < 0.00001, I2 = 58%, very low certainty evidence). For women without diabetes, there was insufficient evidence to determine the effect of tight intrapartum glycaemic control on neonatal hypoglycaemia.

Conclusions
Very uncertain evidence suggests that tight intrapartum glycaemic control may reduce neonatal hypoglycaemia in infants of women with diabetes. High-quality RCTs are required.
Examining the etiological factors resulting in retinal detachment following prophylactic vitrectomy in the context of acute retinal necrosis syndrome
Authors: Nie Yu-hong , Zhang Yu , Chen Zhen , Xing Yi-qiao 
Published: June 2024

Abstract
Objective
The aim of this study is to elucidate the factors contributing to the occurrence of retinal detachment (RD) following prophylactic vitrectomy in cases of acute retinal necrosis (ARN) syndrome.

Methods
A retrospective examination was undertaken, encompassing the medical records of patients diagnosed with ARN who underwent prophylactic vitreous intervention at the Ophthalmology Department of Wuhan University Renmin Hospital East Campus between October 2019 and September 2023. Subsequently, patients who manifested RD in the postoperative period were identified, and a comprehensive analysis was conducted to ascertain the factors underlying the occurrence of RD post-surgery.

Results
This study comprised 14 cases (involving 14 eyes) of patients diagnosed with ARN who underwent prophylactic vitreous intervention. The findings revealed that 4 patients experienced postoperative RD, resulting in an incidence rate of 28.57%. Notably, among these cases, 3 cases of RD manifested in the presence of silicone oil, while 1 case occurred subsequent to the removal of silicone oil. All 4 cases of RD exhibited varied degrees of proliferative vitreoretinopathy. Following the occurrence of RD, all patients underwent a secondary vitreous intervention coupled with silicone oil tamponade, leading to successful reattachment of the retina. However, despite these interventions, there was no significant enhancement observed in postoperative visual outcomes when compared to preoperative levels.

Conclusion
RD following prophylactic vitrectomy in cases of ARN is not an infrequent occurrence and is primarily linked to the postoperative onset of proliferative vitreoretinopathy.
Prognostic and predictive value of examined lymph node count in stage III colorectal cancer: a population based study
Authors: Wei Ran , Zheng Zifan , Li Qinghai , Qian Yan , Wu Chong , Li Yin , Wang Mian , Chen Jianhui , He Weiling 
Published: June 2024

Abstract
Background
The role of tumor-draining lymph nodes in the progression of malignant tumors, including stage III colorectal cancer (CRC), is critical. However, the prognostic and predictive value of the number of examined lymph nodes (ELNs) are not fully understood.

Methods
This population-based study retrospectively analyzed data from 106,843 patients with stage III CRC who underwent surgical treatment and registered in three databases from 2004 to 2021. The Surveillance, Epidemiology, and End Results (SEER) cohort was divided using into training and test cohorts at a ratio of 3:2. We employed restricted cubic spline (RCS) curves to explore nonlinear relationships between overall survival (OS) and ELNs counts and performed Cox regression to evaluate hazard ratios across different ELNs count subtypes. Additional validation cohorts were utilized from the First Affiliated Hospital, Sun Yat-sen University and The Cancer Genome Atlas (TCGA) under the same criteria. Outcomes measured included OS, cancer-specific survival (CSS), and progression-free survival (PFS). Molecular analyses involved differential gene expression using the “limma” package and immune profiling through CIBERSORT. Tissue microarray slides and multiplex immunofluorescence (MIF) were used to assess protein expression and immune cell infiltration.

Results
Patients with higher ELNs counts (≥ 17) demonstrated significantly better long-term survival outcomes across all cohorts. Enhanced OS, CSS, and PFS were notably evident in the LN-ELN group compared to those with fewer ELNs. Cox regression models underscored the prognostic value of higher ELNs counts across different patient subgroups by age, sex, tumor differentiation, and TNM stages. Subtype analysis based on ELNs count revealed a marked survival benefit in patients treated with adjuvant chemotherapy in the medium and large ELNs counts (≥ 12), whereas those with fewer ELNs showed negligible benefits. RNA sequencing and MIF indicated elevated immune activation in the LN-ELN group, characterized by increased CD3+, CD4+, and CD8 + T cells within the tumor microenvironment.

Conclusions
The number of ELNs independently predicts survival and the immunological landscape at the tumor site in stage III CRC, underscoring its dual prognostic and predictive value.
Effect of COVID-19 response work experience on turnover intention among employees of dedicated COVID-19 hospitals in Seoul
Authors: Park Eunyoung , You Chang Hoon , Joung Hyojee , Kwon Young Dae 
Published: June 2024

Abstract
Background
According to previous studies, stress and job burnout among medical personnel increased during the COVID-19 pandemic. This study analyzed the effect of the experience of COVID-19 response work on the intention of municipal hospital staffs to leave their workplaces during the pandemic.

Methods
The 3556 employees who had worked for more than 1 year at one of the eight Seoul Municipal Hospitals that either provided inpatient treatment for quarantined COVID-19 patients or operated as screening clinics were taken as the study population. In total, 1227 employees completed a web or mobile survey between October 21 and November 18, 2020. A chi-squared test was performed to confirm the difference in the distribution of turnover intention depending on whether the employees performed COVID-19 response tasks. Multiple logistic regression analyses were performed to determine the factors that affected the intention to leave.

Results
Of the 1227 respondents, 761 (62.0%) were frontline workers who were the first line of response to COVID-19. Experience with COVID-19 response tasks (OR = 1.59, p = 0.003) was significantly associated with the intention to leave. Additionally, the probability of turnover intention was significantly higher among workers aged 20–29 years (OR = 2.11, p = 0.038) and 40–49 years (OR = 1.57, p = 0.048), unmarried individuals (OR = 1.66, p = 0.005), doctors (OR = 2.41, p = 0.010), nurses (OR = 1.59, p = 0.036), and technical staff members (OR = 2.22, p = 0.009). High turnover intention was found among those who experienced high levels of burnout (OR = 2.03, p < 0.001) and those working in non-directly managed municipal hospitals (OR = 1.87, p = 0.018).

Conclusion
Employees directly involved in COVID-19 response work displayed higher turnover intention. Various personal, job, and organizational factors significantly influenced employees’ intentions to leave their positions in dedicated COVID-19 hospitals. These findings suggest the necessity of introducing management programs to aid workers who have experienced sudden changes in their duties and loss of autonomy while performing COVID-19 response tasks.
BI-2865, a pan-KRAS inhibitor, reverses the P-glycoprotein induced multidrug resistance in vitro and in vivo
Authors: Yang Qihong , To Kenneth Kin Wah , Hu Guilin , Fu Kai , Yang Chuan , Zhu Shuangli , Pan Can , Wang Fang , Luo Kewang , Fu Liwu 
Published: June 2024

Abstract
Background
Multidrug resistance (MDR) limits successful cancer chemotherapy. P-glycoprotein (P-gp), BCRP and MRP1 are the key triggers of MDR. Unfortunately, no MDR modulator was approved by FDA to date. Here, we will investigate the effect of BI-2865, a pan-KRAS inhibitor, on reversing MDR induced by P-gp, BCRP and MRP1 in vitro and in vivo, and its reversal mechanisms will be explored.

Methods
The cytotoxicity of BI-2865 and its MDR removal effect in vitro were tested by MTT assays, and the corresponding reversal function in vivo was assessed through the P-gp mediated KBv200 xenografts in mice. BI-2865 induced alterations of drug discharge and reservation in cells were estimated by experiments of Flow cytometry with fluorescent doxorubicin, and the chemo-drug accumulation in xenografts’ tumor were analyzed through LC-MS. Mechanisms of BI-2865 inhibiting P-gp substrate’s efflux were analyzed through the vanadate-sensitive ATPase assay, [125I]-IAAP-photolabeling assay and computer molecular docking. The effects of BI-2865 on P-gp expression and KRAS-downstream signaling were detected via Western blotting, Flow cytometry and/or qRT-PCR. Subcellular localization of P-gp was visualized by Immunofluorescence.

Results
We found BI-2865 notably fortified response of P-gp-driven MDR cancer cells to the administration of chemo-drugs including paclitaxel, vincristine and doxorubicin, while such an effect was not observed in their parental sensitive cells and BCRP or MRP1-driven MDR cells. Importantly, the mice vivo combination study has verified that BI-2865 effectively improved the anti-tumor action of paclitaxel without toxic injury. In mechanism, BI-2865 prompted doxorubicin accumulating in carcinoma cells by directly blocking the efflux function of P-gp, which more specifically, was achieved by BI-2865 competitively binding to the drug-binding sites of P-gp. What’s more, at the effective MDR reversal concentrations, BI-2865 neither varied the expression and location of P-gp nor reduced its downstream AKT or ERK1/2 signaling activity.

Conclusions
This study uncovered a new application of BI-2865 as a MDR modulator, which might be used to effectively, safely and specifically improve chemotherapeutic efficacy in the clinical P-gp mediated MDR refractory cancers.
Distinct characteristics of the gut virome in patients with osteoarthritis and gouty arthritis
Authors: Chen Chang-Ming , Yan Qiu-Long , Guo Ruo-Chun , Tang Fang , Wang Min-Hui , Yi Han-Zhi , Huang Chun-Xia , Liu Can , Wang Qiu-Yi , Lan Wei-Ya , Jiang Zong , Yang Yu-Zheng , Wang Guang-Yang , Zhang Ai-Qin , Ma Jie , Zhang Yan , You Wei , Ullah Hayan , Zhang Yue , Li Sheng-Hui , Yao Xue-Ming , Sun Wen , Ma Wu-Kai 
Published: June 2024

Abstract
Background/purpose(s)
The gut microbiota and its metabolites play crucial roles in pathogenesis of arthritis, highlighting gut microbiota as a promising avenue for modulating autoimmunity. However, the characterization of the gut virome in arthritis patients, including osteoarthritis (OA) and gouty arthritis (GA), requires further investigation.

Methods
We employed virus-like particle (VLP)-based metagenomic sequencing to analyze gut viral community in 20 OA patients, 26 GA patients, and 31 healthy controls, encompassing a total of 77 fecal samples.

Results
Our analysis generated 6819 vOTUs, with a considerable proportion of viral genomes differing from existing catalogs. The gut virome in OA and GA patients differed significantly from healthy controls, showing variations in diversity and viral family abundances. We identified 157 OA-associated and 94 GA-associated vOTUs, achieving high accuracy in patient-control discrimination with random forest models. OA-associated viruses were predicted to infect pro-inflammatory bacteria or bacteria associated with immunoglobulin A production, while GA-associated viruses were linked to Bacteroidaceae or Lachnospiraceae phages. Furthermore, several viral functional orthologs displayed significant differences in frequency between OA-enriched and GA-enriched vOTUs, suggesting potential functional roles of these viruses. Additionally, we trained classification models based on gut viral signatures to effectively discriminate OA or GA patients from healthy controls, yielding AUC values up to 0.97, indicating the clinical utility of the gut virome in diagnosing OA or GA.

Conclusion
Our study highlights distinctive alterations in viral diversity and taxonomy within gut virome of OA and GA patients, offering insights into arthritis etiology and potential treatment and prevention strategies.

Graphical Abstract