bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel vascular targeting strategy for brain‐derived endothelial cells using a TCR mimic antibody
Authors: Bhattacharya Raktima , Xu Yan , Rahman Md. Ashequr , Couraud Pierre‐Olivier , Romero Ignacio A. , Weksler Babette B. , Weidanz Jon A. , Bickel Ulrich 
Published: May 2010

AbstractOrgan‐specific vascular targeting, for example, to the blood–brain barrier, requires the identification of unique molecular addresses on a subset of endothelial cells. The present study describes a crucial step towards tapping the exquisite specificity of the peptide/HLA class I system for this goal. We utilized a novel T‐cell receptor (TCR) mimic antibody of high affinity and specificity, which is restricted by HLA‐A2 and has been generated to recognize a peptide epitope derived from p68 RNA helicase (YLLPAIVHI). The parent protein is highly expressed by brain endothelial cells. Flow cytometry and confocal imaging showed that the antibody binds to HLA‐A2‐positive human brain‐derived endothelial cells, both immortalized hCMEC/D3 cells and primary cells. The TCR mimic antibody undergoes internalization into vesicles, where significant colocalization occurs with the early endosomal marker EEA‐1, but barely with caveolin‐1. To our knowledge internalization of neither MHC class I protein nor TCR mimics by brain endothelial cells has been previously observed. Knock down of p68 protein expression by siRNA reduced the presentation of YLLPAIVHI‐peptide/HLA‐A2 complexes on the cell membrane by half as measured by flow cytometry 48 h later. We also found that brain endothelial cells isolated from HLA‐A2 transgenic mouse strains express the A2 transgene, and brain endothelial cells of one of these strains also present YLLPAIVHI‐peptide/HLA‐A2, making these mouse strains suitable models for studying TCR mimic antibodies in vivo. In conclusion, these data strongly support the notion that TCR mimic antibodies could be a new class of therapeutic targeting agents in a wide variety of diseases. J. Cell. Physiol. 225: 664–672, 2010. © 2010 Wiley‐Liss, Inc.
Shed HER2 extracellular domain in HER2‐mediated tumor growth and in trastuzumab susceptibility
Authors: Ghedini Gaia C. , Ciravolo Valentina , Tortoreto Monica , Giuffrè Sarah , Bianchi Francesca , Campiglio Manuela , Mortarino Mimosa , Figini Mariangela , Coliva Angela , Carcangiu Maria L. , Zambetti Milvia , Piazza Tiziana , Ferrini Silvano , Ménard Sylvie , Tagliabue Elda , Pupa Serenella M. 
Published: May 2010

AbstractThe question of the serum HER2 extracellular domain (HER2/ECD) measurement for prediction of response to the anti‐HER2 antibody Trastuzumab is still an open and current matter of clinical debate. To elucidate the involvement of shed HER2/ECD in HER2‐driven tumor progression and in guiding therapy of individual patients, we examined biological effects exerted by elevated HER2/ECD in cancer growth and in response to Trastuzumab. To this purpose SKOV3 tumor cells were stably transfected to release a recombinant HER2/ECD molecule (rECD). Transfectants releasing high levels of 110‐kDa rECD, identical in size to native HER2/ECD (nECD), grew significantly slower than did controls, which constitutively released only basal levels of nECD. While transmembrane HER2 and HER1 were expressed at equal levels by both controls and transfected cells, activation of these molecules and of downstream ERK2 and Akt was significantly reduced only in rECD transfectants. Surface plasmon resonance analysis revealed heterodimerization of the rECD with HER1, ‐2, and ‐3. In cell growth bioassays in vitro, shed HER2 significantly blocked HER2‐driven tumor cell proliferation. In mice, high levels of circulating rECD significantly impaired HER2‐driven SKOV3 tumor growth but not that of HER2‐negative tumor cells. In vitro and in mice, Trastuzumab significantly inhibited tumor growth due to the rECD‐facilitated accumulation of the antibody on tumor cells. Globally our findings sustain the biological relevance of elevated HER2/ECD levels in the outcome of HER2‐disease and in the susceptibility to Trastuzumab‐based therapy. J. Cell. Physiol. 225: 256–265, 2010. © 2010 Wiley‐Liss, Inc.
Ultrasound‐assisted dispersive liquid–liquid microextraction coupled with capillary gas chromatography for simultaneous analysis of nine pyrethroids in domestic wastewaters
Authors: Yan Hongyuan , Du Jingjing , Zhang Xiguo , Yang Gengliang , Row Kyung Ho , Lv Yunkai 
Published: May 2010

AbstractA simple and rapid ultrasound‐assisted dispersive liquid–liquid microextraction method coupled with GC‐flame ionization detection was developed for simultaneous determination of nine pyrethroids in domestic wastewater samples. An ultrasound‐assisted process was applied to accelerate the formation of the fine cloudy solution using small volume of disperser solvent, which markedly increased the extraction efficiency and reduced the equilibrium time. Various parameters affecting the extraction efficiency were investigated, including the type and volume of extraction solvent and disperser solvent, extraction and ultrasonic time. Good linearity was obtained for all analytes in the range of 0.8–100 μg/L with the correlation coefficient (r2)≥0.998. The recoveries at three spiking levels ranged from 75.3 to 101.2% with the RSD less than 8.7% (n=5). Under the optimum condition, the enrichment factors for the nine pyrethroids ranged from 728‐ to 1725‐fold. This method offered a good alternative for routine analysis due to its simplicity and reliability.
Sample complexity reduction aids efficient detection of low‐abundant proteins from human amniotic fluid
Authors: Alam Mashkoor , Selladurai Masilamani , Nagpal Saurabh , Tomar Anil Kumar , Saraswat Mayank , Raziuddin M. , Mittal Sunita , Singh Tej P. , Yadav Savita 
Published: May 2010

AbstractWorking with biological fluids poses a challenge of visualizing proteins present in lower concentrations. This study describes a batch‐mode chromatographic method for the fractionation of human amniotic fluid (AF). This method is easy to use with minimal sample quantity, resin volume and sample processing time. For albumin depletion, two methods were evaluated. The results demonstrated that specific depletion of albumin, using affinity‐ligand‐based resin, is more efficient than the conventional dye‐based method. The albumin‐depleted human AF was fractionated by strong anion‐exchange resin in spin devices, for sample, complexity reduction and enrichment of low‐abundant proteins. Analysis of four eluate fractions generated after this step shows enrichment of few low‐abundant proteins. Two novel low‐abundant proteins, Rab GDP dissociation inhibitor β and peptide methionine sulfoxide reductase, were identified from human AF. α‐1‐B Glycoprotein was successfully identified by this strategy, whereas the published literature reports that it was not identified by strong anion‐exchange FPLC followed by SDS‐PAGE. Therefore, the current method has distinct advantages over the conventional column‐based chromatography. This study also reports altered expression of some proteins in Rh‐isoimmunized AF samples in comparison with normal AF.
Analysis of cocaine and benzoylecgonine in urine by using multisyringe flow injection analysis‐gas chromatography‐mass spectrometry system
Authors: Brunetto María del Rosario , Delgado Yelitza , Clavijo Sabrina , Contreras Yaritza , Torres Dina , Ayala Carlos , Gallignani Máximo , Forteza Rafael , Martin Víctor Cerdà 
Published: May 2010

AbstractIn this paper, a method was described to determine cocaine (COC) and benzoylecgonine (BZE) in human urine samples by GC‐MS detection. The extraction of analytes from urine samples was achieved in an Oasis hydrophilic–lipophilic balance column (20 mm×3.9 mm id, dp=25 μm; Waters, USA), incorporated in a multisyringe flow injection system, used for the sample treatment. Finally, to improve the volatility of the BZE, an in‐line derivatization reaction withN,O‐bis(trimethylsilyl) trifluoroacetamide with 1% trimethylchlorosilane was made microwave‐assisted in order to reduce the reaction time. The results showed that the proposed method is a good alternative for the analysis of COC and BZE in urine samples because it offers advantages compared with those described in the literature, which include simplicity in the sample treatment, the sensitivity and selectivity necessary to determine the analytes of interest at low levels in the urine and high sample throughput.
Application of accelerated solvent extraction to the investigation of saikosaponins from the roots of Bupleurum falcatum
Authors: Li Wei , Liu Zhengbo , Wang Zi , Chen Li , Sun Yinshi , Hou Jingang , Zheng Yinan 
Published: May 2010

AbstractAccelerated solvent extraction (ASE) was applied to the extraction of saikosaponin a, saikosaponin c and saikosaponin d from the roots of Bupleurum falcatum. Main extraction parameters such as the extraction solvents, extraction temperature and static extraction time were investigated and optimized. The optimized procedure employed 70% methanol as extraction solvent, 120°C of extraction temperature, 10 min of static extraction time, 60% of flush volume and the extraction recoveries of the three compounds were near to 100% with one extraction cycle. The extracted samples were analyzed by HPLC with UV detector. The HPLC conditions were as follows: Hypersil ODS2 (4.6 mm×250 mm, 5 μm) column, acetonitrile and water as mobile phase, flow rate of 1.0 mL/min, UV detection wavelength of 204 nm and injection volume of 20 μL. Compared with the traditional methods including heat‐reflux extraction and ultrasonic‐assisted extraction, the proposed ASE method was more efficient and faster to be operated. The results indicated that ASE was an alternative method for extracting saikosaponins from the roots of B. falcatum.
Multi‐residue method for the analysis of pharmaceutical compounds in sewage sludge, compost and sediments by sonication‐assisted extraction and LC determination
Authors: Martín Julia , Santos Juan Luis , Aparicio Irene , Alonso Esteban 
Published: May 2010

AbstractA method for the simultaneous determination of 16 pharmaceutical compounds in three types of sewage sludge (primary, secondary and anaerobically digested dehydrated sludge), compost and sediment samples is described. Pharmaceutical compounds evaluated were nonsteroidal anti‐inflammatory drugs (acetaminophen, diclofenac, ibuprofen, ketoprofen, naproxen and salicylic acid), antibiotics (sulfamethoxazole and trimethoprim), an anti‐epileptic drug (carbamazepine), a β‐blocker (propranolol), a nervous stimulant (caffeine), estrogens (17α‐ethinylestradiol, 17β‐estradiol, estriol and estrone) and lipid regulators (clofibric acid, metabolite of clofibrate and gemfibrozil). The method is based on the ultrasonic‐assisted extraction, clean‐up by SPE and analytical determination by HPLC with diode array and fluorescence detectors. The best extraction recoveries were achieved in a three‐step extraction procedure with methanol and acetone as extraction solvents. Extraction recoveries of several pharmaceutical compounds as caffeine were highly dependent on the type of sample evaluated. The applicability of the method was tested by analyzing primary, secondary and anaerobically digested dehydrated sludge, compost and sediment samples from Seville (Southern Spain). Ten of the sixteen pharmaceutical compounds were detected in sludge samples and five in compost and sediment samples. The highest concentration levels were recorded for ibuprofen in sewage samples, whereas salicylic acid and 17α‐ethinylestradiol were detected in all of the samples analyzed.
Micellar and aqueous‐organic liquid chromatography using sub‐2 μm packings for fast separation of natural phenolic compounds
Authors: Cao Jun , Qu Haibin , Cheng Yiyu 
Published: May 2010

AbstractThe objective of the present work was to investigate the chromatographic behavior of natural phenolic compounds in micellar and aqueous‐organic LC using a short column packed with 1.8 μm particles. Firstly, the effect of ACN and SDS on elution strength and selectivity was examined by isocratic submicellar (0–30% ACN/5% 1‐butanol/1–6 mM SDS) and micellar (0–30% ACN/5% 1‐butanol/40–60 mM SDS) systems. The varied concentrations of two modifiers in the mobile phases revealed different eluting power. Then, the application of organic modifier gradient was discussed in both submicellar and micellar LC using mobile phases of 4 mM SDS/5% 1‐butanol or 50 mM SDS/5% 1‐butanol containing ACN gradient from 0 to 30%, respectively. For micellar system, the separation was found to be better in gradient than isocratic elution. Additionally, the sensitivity of aqueous‐organic LC was examined. The mobile phase was a mixture of ACN and water employing gradient elution at a flow rate of 0.5 mL/min, with analysis time below 9 min. It was found that separation efficiency was significantly better compared with micellar LC. Besides, the aqueous‐organic LC has been applied to separation of various phenolic compounds in Yangwei granule or Radix Astragali samples.
Simultaneous analysis of parabens in cosmetic products by stir bar sorptive extraction and liquid chromatography
Authors: Melo Lidervan P. , Queiroz Maria Eugênia C. 
Published: May 2010

AbstractA sensitive and precise stir bar sorptive extraction (SBSE) combined with LC (SBSE/LC) analysis is described for simultaneous determination of methyl, ethyl, propyl, and butyl parabens in commercial cosmetic products in agreement with the European Union Cosmetics Directive 76/768/EEC. Important factors in the optimization of SBSE efficiency are discussed, such as time and temperature of extraction, pH, and ionic strength of the sample, matrix effects, and liquid desorption conditions by different modes (magnetic stirring, ultrasonic). The LOQs of the SBSE/LC method ranged from 30 to 200 ng/mg, with linear response over a dynamic range, from the LOQ to 2.5 μg/mg, with a coefficient of determination higher than 0.993. The interday precision of the SBSE/LC method presented a coefficient of variation lower than 5%. The effectiveness of the proposed method was proven for analysis of commercial cosmetic products such as body creams, antiperspirant creams, and sunscreens.
Temperature‐controlled ionic liquid dispersive liquid‐phase microextraction for the sensitive determination of triclosan and triclocarban in environmental water samples prior to HPLC‐ESI‐MS/MS
Authors: Zhao Ru‐Song , Wang Xia , Sun Jing , Yuan Jin‐Peng , Wang Shan‐Shan , Wang Xi‐Kui 
Published: May 2010

AbstractA novel dispersive liquid‐phase microextraction method without dispersive solvents has been developed for the enrichment and sensitive determination of triclosan and triclocarban in environmental water samples prior to HPLC‐ESI‐MS/MS. This method used only green solvent 1‐hexyl‐3‐methylimidazolium hexafluorophosphate as extraction solvent and overcame the demerits of the use of toxic solvents and the instability of the suspending drop in single drop liquid‐phase microextraction. Important factors that may influence the enrichment efficiencies, such as volume of ionic liquid, pH of solutions, extraction time, centrifuging time and temperature, were systematically investigated and optimized. Under optimum conditions, linearity of the method was observed in the range of 0.1–20 μg/L for triclocarban and 0.5–100 μg/L for triclosan, respectively, with adequate correlation coefficients (R>0.9990). The proposed method has been found to have excellent detection sensitivity with LODs of 0.04 and 0.3 μg/L, and precisions of 4.7 and 6.0% (RSDs, n=5) for triclocarban and triclosan, respectively. This method has been successfully applied to analyze real water samples and satisfactory results were achieved.
Beneficial effects of vaccenic acid on postprandial lipid metabolism and dyslipidemia: Impact of natural trans‐fats to improve CVD risk
Authors: Wang Ye , Jacome‐Sosa M. Miriam , Vine Donna F. , Proctor Spencer D. 
Published: May 2010

AbstractAbnormal non‐fasting (postprandial) lipid metabolism has been recognized as a significant contributor to dyslipidemia and cardiovascular disease (CVD) risk. Clinically, impaired metabolism of lipoproteins following a meal (e.g. chylomicrons) has been demonstrated in a number of chronic diseases, including obesity, insulin resistance, as well as type 1 and 2 diabetes. Given the proposed effects of dietary trans fat to contribute to a lipid profile that increases CVD risk, there has been a public health campaign in many countries to eliminate these fatty acids from the food supply. In contrast, our group has recently reported novel lipid‐lowering benefits of a major naturally‐occurring trans fatty acid vaccenic acid (VA, shorthand lipid name 18:1 trans‐11), in an animal model of dyslipidemia and the metabolic syndrome. Studies to date have shown that dietary supplementation of VA effectively reduces not only fasting lipids, but also postprandial triacylglycerol and chylomicron concentrations in obese JCR:LA‐cp rats. Evidence from animal studies to date suggest that VA may down‐regulate hepatic fatty acid synthesis and directly influence lipogenesis in the intestine. The discovery of new bioactive properties of VA is supported by clinical studies which have provided increased momentum for industry applications. In this review we summarize the emerging beneficial view of natural trans fats that have distinct and differential properties compared to those synthetically produced in partially hydrogenated vegetable oils (PHVO), with a particular focus on fasting and postprandial lipid metabolism in CVD risk.
Stability of fat‐soluble vitamins and PUFA in simulated shallow‐frying
Authors: Hrncirik Karel 
Published: May 2010

AbstractFat‐soluble vitamins and essential polyunsaturated fatty acids (PUFA) are nutrients that are sensitive to heat treatment and oxidation and therefore can be partly lost during cooking. It is well‐documented that a long‐term deep‐frying process leads to a substantial degradation of PUFA and vitamins in a frying medium. In this study, the stability of vitamins and fatty acids was investigated in a series of liquid margarines (82% fat) varying in fat blend and the content of minor components. The tested samples were subjected to the standardised treatment that has been developed to mimic shallow‐frying of food at 180°C and the recovery of individual nutrients is reported. The results show that fat‐soluble vitamins possessed reasonable stability under the condition of the shallow‐frying test. Essential PUFA of the frying medium were hardly affected by the treatment. Overall evaluation of the results suggests that oil‐based cooking media rich in vitamins and PUFA have a positive impact on nutritional value of food prepared by appropriate means of cooking.
Dietary cholesterol, blood cholesterol and cardiovascular disease
Authors: Stanley John 
Published: May 2010

AbstractCholesterol is an essential cellular nutrient and homeostatic mechanisms exist to balance rates of cholesterol production and utilisation and minimise effects of dietary cholesterol on blood cholesterol and hence cardiovascular disease risk. In non‐diabetic populations, there is little evidence from epidemiological studies for associations between dietary cholesterol or the consumption of cholesterol rich foods and cardiovascular disease risk, or from intervention trials for major effects of dietary cholesterol on blood cholesterol levels. By contrast, evidence is emerging from epidemiological studies that consumption of eggs is associated with cardiovascular disease in diabetics, and from intervention trials that dietary cholesterol has greater effects on blood cholesterol levels in insulin resistant people. Since insulin plays a role in cholesterol homeostasis, these are not surprising findings.
Racial and ethnic disparities in access to liver transplantation
Authors: Mathur Amit K. , Schaubel Douglas E. , Gong Qi , Guidinger Mary K. , Merion Robert M. 
Published: May 2010

Abstract
Access to liver transplantation is reportedly inequitable for racial/ethnic minorities, but inadequate adjustments for geography and disease progression preclude any meaningful conclusions. We aimed to evaluate the association between candidate race/ethnicity and liver transplant rates after thorough adjustments for these factors and to determine how uniform racial/ethnic disparities were across Model for End-Stage Liver Disease (MELD) scores. Chronic end-stage liver disease candidates initially wait-listed between February 28, 2002 and February 27, 2007 were identified from Scientific Registry for Transplant Recipients data. The primary outcome was deceased donor liver transplantation (DDLT); the primary exposure covariate was race/ethnicity (white, African American, Hispanic, Asian, and other). Cox regression was used to estimate the covariate-adjusted DDLT rates by race/ethnicity, which were stratified by the donation service area and MELD score. With averaging across all MELD scores, African Americans, Asians, and others had similar adjusted DDLT rates in comparison with whites. However, Hispanics had an 8% lower DDLT rate versus whites [hazard ratio (HR) = 0.92, P = 0.011]. The disparity among Hispanics was concentrated among patients with MELD scores < 20, with HR = 0.84 (P = 0.021) for MELD scores of 6 to 14 and HR = 0.85 (P = 0.009) for MELD scores of 15 to 19. Asians with MELD scores < 15 had a 24% higher DDLT rate with respect to whites (HR = 1.24, P = 0.024). However, Asians with MELD scores of 30 to 40 had a 46% lower DDLT rate (HR = 0.54, P = 0.004). In conclusion, although African Americans did not have significantly different DDLT rates in comparison with similar white candidates, race/ethnicity-based disparities were prominent among subgroups of Hispanic and Asian candidates. By precluding the survival benefit of liver transplantation, this inequity may lead to excess mortality for minority candidates. Liver Transpl 16:1033-1040, 2010. © 2010 AASLD.
Interaction of Hsp27 with Native Phosphorylase Kinase under Crowding Conditions
Authors: Chebotareva Natalia A. , Makeeva Valentina F. , Bazhina Svetlana G. , Eronina Tatyana B. , Gusev Nikolai B. , Kurganov Boris I. 
Published: May 2010

AbstractInteraction of the wild type (wt) heat shock protein Hsp27 and its three‐dimensional (3D) mutant (mimicking phosphorylation at Ser15, 78, and 82) with rabbit skeletal muscle phosphorylase kinase (PhK) has been studied under crowding conditions modeled by addition of 1 M trimethylamine N‐oxide (TMAO). According to the data of sedimentation velocity and dynamic light scattering, crowding provokes the formation of large‐sized associates of both PhK and Hsp27. Under crowding conditions, small associates of PhK and Hsp27 interact with each other thus leading to dissociation of large homooligomers of each protein. Taking into account high concentrations of PhK in the cell, we speculate that native PhK might modulate the oligomeric state and chaperone‐like activity of Hsp27.magnified image
Preparation of Collagen‐Glycosaminoglycan Sponges with Open Surface Porous Structures Using Ice Particulate Template Method
Authors: Ko Young‐Gwang , Grice Sarah , Kawazoe Naoki , Tateishi Tetsuya , Chen Guoping 
Published: May 2010

AbstractFunnel‐like sponges of collagen incorporated with glycosaminoglycan (GAG) were prepared by freeze‐drying using ice particulates as templates. The funnel‐like collagen‐GAG sponges showed similar porous structures to those of funnel‐like collagen sponges. The funnel‐like collagen‐GAG and collagen sponges have one top surface layer and one bulk porous layer. The large, top surface pores were determined by ice particulates that were used as templates, and the inner bulk pores were determined by freezing temperature. The funnel‐like pore structures facilitated homogenous cell distribution, improved cell viability, and resulted in homogenous tissue formation. Incorporation of GAG increased the mechanical property and cell viability of collagen sponges.magnified image