bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conventional and pretargeted radioimmunotherapy using bismuth-213 to target and treat non-Hodgkin lymphomas expressing CD20: a preclinical model toward optimal consolidation therapy to eradicate minimal residual disease
Authors: Park Steven I. , Shenoi Jaideep , Pagel John M. , Hamlin Don K. , Wilbur D. Scott , Orgun Nural , Kenoyer Aimee L. , Frayo Shani , Axtman Amanda , Bäck Tom , Lin Yukang , Fisher Darrell R. , Gopal Ajay K. , Green Damian J. , Press Oliver W. 
Published: August 2010

Abstract
Radioimmunotherapy (RIT) with α-emitting radionuclides is an attractive approach for the treatment of minimal residual disease because the short path lengths and high energies of α-particles produce optimal cytotoxicity at small target sites while minimizing damage to surrounding normal tissues. Pretargeted RIT (PRIT) using antibody-streptavidin (Ab-SA) constructs and radiolabeled biotin allows rapid, specific localization of radioactivity at tumor sites, making it an optimal method to target α-emitters with short half-lives, such as bismuth-213 (213Bi). Athymic mice bearing Ramos lymphoma xenografts received anti-CD20 1F5(scFv)4SA fusion protein (FP), followed by a dendrimeric clearing agent and [213Bi]DOTA-biotin. After 90 minutes, tumor uptake for 1F5(scFv)4SA was 16.5% ± 7.0% injected dose per gram compared with 2.3% ± .9% injected dose per gram for the control FP. Mice treated with anti-CD20 PRIT and 600 μCi [213Bi]DOTA-biotin exhibited marked tumor growth delays compared with controls (mean tumor volume .01 ± .02 vs. 203.38 ± 83.03 mm3 after 19 days, respectively). The median survival for the 1F5(scFv)4SA group was 90 days compared with 23 days for the control FP (P < .0001). Treatment was well tolerated, with no treatment-related mortalities. This study demonstrates the favorable biodistribution profile and excellent therapeutic efficacy attainable with 213Bi-labeled anti-CD20 PRIT.
Proteomic characterization of an isolated fraction of synthetic proteasome inhibitor (PSI)-induced inclusions in PC12 cells might offer clues to aggresomes as a cellular defensive response against proteasome inhibition by PSI
Authors: Li Xing'an , Zhang Yingjiu , Xie Peng , Piao Jinhua , Hu Yihong , Chang Ming , Liu Tao , Hu Linsen 
Published: August 2010

AbstractBackgroundCooperation of constituents of the ubiquitin proteasome system (UPS) with chaperone proteins in degrading proteins mediate a wide range of cellular processes, such as synaptic function and neurotransmission, gene transcription, protein trafficking, mitochondrial function and metabolism, antioxidant defence mechanisms, and apoptotic signal transduction. It is supposed that constituents of the UPS and chaperone proteins are recruited into aggresomes where aberrant and potentially cytotoxic proteins may be sequestered in an inactive form.ResultsTo determinate the proteomic pattern of synthetic proteasome inhibitor (PSI)-induced inclusions in PC12 cells after proteasome inhibition by PSI, we analyzed a fraction of PSI-induced inclusions. A proteomic feature of the isolated fraction was characterized by identification of fifty six proteins including twenty previously reported protein components of Lewy bodies, twenty eight newly identified proteins and eight unknown proteins. These proteins, most of which were recognized as a profile of proteins within cellular processes mediated by the UPS, a profile of constituents of the UPS and a profile of chaperone proteins, are classed into at least nine accepted categories. In addition, prolyl-4-hydroxylase beta polypeptide, an endoplasmic reticulum member of the protein disulfide isomerase family, was validated in the developmental process of PSI-induced inclusions in the cells.ConclusionsIt is speculated that proteomic characterization of an isolated fraction of PSI-induced inclusions in PC12 cells might offer clues to appearance of aggresomes serving as a cellular defensive response against proteasome inhibition.
Malaria risk in Corsica, former hot spot of malaria in France
Authors: Toty Céline , Barré Hélène , Le Goff Gilbert , Larget-Thiéry Isabelle , Rahola Nil , Couret Daniel , Fontenille Didier 
Published: August 2010

AbstractBackgroundThe prevalence ofPlasmodium falciparumandPlasmodium vivaxmalaria was very high in Corsica just before the Second World War. The last outbreak was in 1972 and the most recent indigenous case was in 2006.ResultsAnalysis of historical data shows that anopheline vectors were abundant. Recent surveys demonstrated that potential vectors are still present in Corsica, despite the likely disappearance ofAnopheles sacharovi. Moreover,P. falciparumcan develop experimentally into these mosquitoes, notablyAnopheles labranchiae, which is locally abundant, and parasites are regularly introduced into the island.Discussion, ConclusionsThe presence of vectors, the introduction of parasites and the conducive climate raise questions about the possibility of malaria re-emerging and becoming re-established in Corsica. Analysis of historic and current parasitological and entomological data shows that the current theoretical risk of indigenous cases or malaria foci is negligible, particularly since there is very little contact between humans andAnophelesmosquitoes,Plasmodiumcarriers are reliably treated and there is a widespread vector control on the island.
Endogenous Activation of Glucokinase by 6-Phosphofructo-2-Kinase/Fructose-2,6-Bisphosphatase Is Glucose Dependent
Authors: Langer Sara , Kaminski Martin T. , Lenzen Sigurd , Baltrusch Simone 
Published: August 2010

Abstract
Glucokinase (GK) plays a crucial role as glucose sensor in glucose-induced insulin secretion in pancreatic β-cells. The bifunctional enzyme 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (PFK-2/FBPase-2) acts as an endogenous GK activator. Therefore, the goal of this study was the analysis of GK-PFK-2/FBPase-2 complex formation and its effect on metabolic stimulus-secretion coupling in β-cells in dependence upon glucose. The interaction between GK and PFK-2/FBPase-2 was analyzed in insulin-secreting MIN6 cells with a new fluorescence-based mammalian two-hybrid system. In contrast to the commonly used mammalian two-hybrid systems that require sampling before detection, the system used allows monitoring of the effects of environmental changes on protein-protein interactions on the single-cell level. Increasing the glucose concentration in the cell culture medium from 3 to 10 and 25 mmol/liter amplified the interaction between the enzymes stepwise. Importantly, in line with these results, overexpression of PFK-2/FBPase-2 in MIN6 cells evoked only at 10 and 25 mmol/liter, an increase in insulin secretion. Furthermore, a PFK-2/FBPase-2 mutant with an abolished GK-binding motif neither showed a glucose-dependent GK binding nor was able to increase insulin secretion. The results obtained with the mammalian two-hybrid system could be confirmed by fluorescence resonance energy transfer experiments in COS cells. Furthermore, the established interaction between GK and the liver GRP served in all experiments as a control. Thus, this study clearly showed that binding and activation of GK by PFK-2/FBPase-2 in β-cells is promoted by glucose, resulting in an enhancement of insulin secretion at stimulatory glucose concentrations, without affecting basal insulin secretion.
Calcium Homeostasis and Skeletal Integrity in Individuals with Familial Hypercholesterolemia and Aortic Calcification
Authors: Awan Zuhier , Alwaili Khalid , AlShahrani Ali , Langsetmo Lisa , Goltzman David , Genest Jacques 
Published: August 2010

BACKGROUND
Familial hypercholesterolemia (FH) due to mutations in the low-density lipoprotein receptor (LDLR) gene exhibit severe, premature aortic calcification in a gene-dosage, age-dependent fashion. We sought to determine potential associations with mineral and skeletal indices.

METHODS
We obtained computed tomography (CT) scan aortic calcium scores (AoCSs) in 19 (age 49 [SD 14] years) FH patients heterozygous for the 15-kb deletion at the LDLR gene and examined associations with various indices of mineral and skeletal homeostasis.

RESULTS
We found that mean bone mineral density (BMD) at the femoral neck in these patients did not differ from age-, sex-, and province-matched mean BMD, and we observed no association of AoCS with any marker of bone resorption. However, there were negative correlations between AoCS and serum concentrations of osteocalcin, a marker of bone formation (r = −0.64, P = 0.0034), urinary calcium (r = −0.59, P = 0.0085), and estimated glomerular filtration rate (r = −0.67, P = 0.0019).

CONCLUSIONS
We found that LDLR-deficient FH was not associated with obvious bone loss or a major disturbance in calcium homeostasis. The lack of LDLR, however, may modify osteoblast function or extracellular calcium distribution, manifesting as lower bone formation, and reduced calcium excretion, resulting in increased deposition in calcifying vascular tissue.
A Consolidation and Contaminant Transport Device for Assessing Reactive Mat Effectiveness for Subaqueous Sediment Remediation
Authors: Meric Dogus , Sheahan Thomas C. , Alshawabkeh Akram , Shine James 
Published: August 2010

Abstract
This paper describes the design and fabrication of a new laboratory testing column to assess the effectiveness of a permeable reactive mat for in situ sediment remediation. After the composite mat, which consists of top and bottom filtering geotextile layers and a middle reactive core, is placed on the sediment surface, hydrodynamic dispersion and pore fluid flow carry contaminants through the mat. In the reactive layer, the contaminants react with one or more amendments. A thin cap of new sediment material can be placed on top of the geocomposite to promote a new, healthy benthic community. The processes controlling these geocomposites have not been physically modeled to assess their effectiveness. The new device is fundamentally a sediment column, but with the capability to apply a constant, overlying stress to the sediment-reactive mat column to cause its consolidation. Pressure-volume controllers at the top and bottom of the sediment column allow in situ pressure conditions to be imposed and allow for chemical sampling of inflow/outflow fluids.
Guided Side-Bend: An Alternative Qualification Method for Butt Fusion Joining of Polyethylene Pipe and Fittings
Authors: Sandstrum Steve , Burwell Dudley , McGriff Steve , Craig Jim , Svetlik Harvey 
Published: August 2010

Abstract
Historically, the procedure for butt fusion joining of polyethylene pipe has been qualified on the basis of two fundamental tests that are widely recognized within the industry. These are the tensile-impact (T-I) test method found in ASTM F2634-07 and the reverse-bend (RB) or bend-back test as described in ASTM F2620-06. Both of these test methods have been extensively utilized within the industry, and both have some specific limitations as it relates to qualification of butt fusion joining conducted in the field. ASTM F2634-07 is a highly sophisticated testing method involving precision instrumented laboratory equipment. This makes it impractical for an on-site qualification for butt fusion procedures in the field. The bend-back test, on the other hand, lends itself to field implementation, but as the method is applied to a larger diameter, heavy-walled pipe, the practicality of performing the test and safety considerations become a limiting factor. This paper will examine an alternative test method referred to as guided side-bend or full-face testing that appears very promising as an alternative to the RB test as described in ASTM F2620-06. The test method will be fully described, and data will be presented comparing T-I results and traditional bend-back results to those obtained using the side-bend test method. The discussion of the test results and safety considerations of the proposed test method will be addressed, and conclusions will be drawn regarding its future applicability.
Identification of several novel non-p.R132 IDH1 variants in thyroid carcinomas
Authors: Hemerly Jefferson Pessoa , Bastos André Uchimura , Cerutti Janete M 
Published: August 2010

ContextSomatic mutations at residue R132 of isocitrate dehydrogenase 1 (IDH1) were recently discovered in gliomas and leukaemia at a high frequency. IDH1 is a metabolic gene, and the R132 mutations create a new enzymatic activity.ObjectivesTo determine whether IDH1 had somatically acquired mutations in thyroid carcinomas.DesignExons 4 and 6 of IDH1 were sequenced in a large panel of thyroid tumours (n=138) and compared with the patients normal DNA (n=26). We also correlated IDH1 mutations with clinical–pathological data and BRAF and RAS mutational status.ResultsWe identified four novel and two previously described non-synonymous variants in thyroid carcinomas, which were absent in benign tumours and paired normal thyroid. Although IDH1 variants occurred at higher frequency in follicular thyroid carcinomas, follicular variant of papillary thyroid carcinoma (PTC) and undifferentiated thyroid carcinomas than the observed variants in classical PTC (15/72 vs 3/37), it was not significant (P=0.1). Sequence alignment across several species shows that all IDH1 genetic alterations occurred at evolutionarily conserved residues located within the active site, and therefore, are likely to affect protein function. Unlike other tumours, IDH1 and BRAF or RAS mutations are not mutually exclusive. There was no association between IDH1 mutational status and clinical characteristics.ConclusionIDH1-acquired genetic alterations are highly prevalent in thyroid carcinomas (16%). Our findings not only extend our understanding of the molecular mechanism underlying pathogenesis of thyroid tumours, but also emphasize the biological differences between tumour types. Those tumours with IDH1 mutations might benefit from therapies that exploit this alteration.
Real-Time Fiber-Optic Casing Imager
Authors: Rambow F. H. , Dria D. E. , Childers B. A. , Appel M.. , Freeman J. J. , Shuck M. Y. , Poland S. H. , Dominique T.. 
Published: August 2010

Summary
During the last several years, significant progress has been made in the use of fiber-optic technology for well and reservoir surveillance. While most effort in this field appears to be concentrated on the development of fiber-optic-based meters for temperature, pressure, and flow, comparably few publications have been made to date about the use of fiber-optic technology for monitoring deformations of well tubulars and casings.
In this article, we report on recent advances in our development of a real-time fiber-optic-based casing imager. This device is designed for continuous, high-resolution monitoring of the shape of casings or well tubulars and, therefore, enables the determination of strain imposed on the well. Small-scale and full-casing-sized laboratory tests have demonstrated that the latest generation of this system is sufficiently sensitive to detect casing deformations of less than 10°/100 ft and covers compressive and tensile axial-strain ranges from less than 0.1 to 10%. We will discuss the background technology, measurement sensitivity and strain-response characterization, as well as the scaleup work that has been performed to date. Our article also includes an overview of field-test results and illustrates how real-time deformation monitoring could form a significant component of reservoir-surveillance strategies.
Sand Cleanouts With Coiled Tubing: Choice of Process, Tools and Fluids
Authors: Li Jeff , Misselbrook John , Sach Manfend 
Published: August 2010

Summary
Cleaning fill from wellbores is the most common coiled tubing (CT) application. The process is a function of multiple variables including fluid properties, flow velocities, wellbore geometry and deviation, pipe eccentricity, particle properties, fill penetration rate and wiper trip speed. Removing fill from wells with low bottom hole pressures (BHP) can be challenging especially if wells are completed with smaller diameter production tubulars which significantly reduce circulation flow cross-section and choke flow(1). Such challenges are further compounded by high deviation or horizontal well trajectories especially in large diameter wellbores.
A variety of cleanout methods have been developed in the past, often incorporating high circulation rates, special fluids, wiper trips, or reverse circulation to remove solids. Many of these conventional sand cleanout methods often apply excess hydrostatic pressure on the formation, resulting in lost circulation in pressure depleted reservoirs. The conventional solution to overcome excess hydrostatic pressure has been to include nitrogen to reduce fluid density and thus lessen the hydrostatic head; however, sand vacuuming technology using a concentric coiled tubing (CCT) with a downhole jet pump is an alternative technique for removing fill without placing a hydrostatic load on the reservoir.
This paper reviews the individual sand cleanout systems and discusses the advantages and limitations related to each method. In recent years, cleaning sand using the wiper trip method has become the preferred technique. However, an appropriate pump rate and reservoir pressure are needed to maintain a proper return flow rate to carry the sands to the surface. For pressure-depleted reservoirs completed with horizontal wells, a sand vacuuming system can be used to efficiently remove the debris without circulating nitrogen and without high pump rates. When the fill cannot be removed from large-diameter deviated wellbores using conventional low-cost cleanout fluids, then fluids with high solids suspension capability (under shear conditions) in conjunction with wiper tripping may be an economical option. The main application for the reverse circulation technique is cleaning sand from large diameter wellbores when the necessary pump rates for conventional "forward" circulation are not achievable. A venturi junk bailer is often used to retrieve larger or heavier material which cannot be circulated out by traditional methods. Field cases are provided, demonstrating how to select the proper cleaning method and how to efficiently remove sand from a wellbore based on both operational and logistical conditions.
Determining Wax Type: Paraffin or Naphthene?
Authors: Bacon Mark M. , Romero-Zerón Laura B. , Chong Ken K. 
Published: August 2010

Summary
Paraffin-wax deposits are known to be a worldwide problem in the upstream petroleum industry. Considerable resources are expended on inhibiting or removing wax deposits every year. Paraffin wax is not the only type of crude-oil-based wax. Microcrystalline waxes, composed of naphthene and iso-paraffin molecules, also exist but are not well studied. The predominance of paraffin (also called macrocrystalline) wax and paraffin-wax-inhibition techniques will likely lead to paraffin-wax treatments being applied to naphthene- based reservoirs. It is unclear how well a paraffin-based treatment will apply to naphthene-based oil.
The current study investigates the use of cross-polarized microscopy (CPM) to determine if a crude oil contains macrocrystalline or microcrystalline waxes. The type of wax present in the crude oil can be determined using crystal morphology and size. This study demonstrates that paraffin-wax crystals are elongated structures with statistically larger sizes than the microcrystals. Microcrystalline waxes are shown to be statistically smaller and rounded structures.
Significant Improvement in the Accuracy of Pressure-Transient Analysis Using Total Least Squares
Authors: Dastan Aysegul , Horne Roland N. 
Published: August 2010

Summary
In this study, we show significant improvements over conventional pressure-transient analysis on the basis of nonlinear regression by using total least squares (TLS), which minimizes errors in both pressure and time simultaneously. To our knowledge, TLS has not been applied to pressure-transient analysis before in this sense.
TLS regression is not an easy problem to solve mathematically, especially for nonlinear pressure-transient-model functions. In this work, we compare four different versions of TLS. We formulate a robust approximation of the TLS solution, which can handle a variety of tests and reservoir models yet does not compromise the performance of the analysis. We show that our technique reduces ambiguity in the estimation of parameters to a large extent, especially in the presence of noise in time. Using our TLS algorithm, we obtain much narrower confidence intervals on the parameter estimates of a variety of real data sets, compared to the conventional least squares (LS) approach. For synthetic data sets, we observe that the TLS estimates are often closer to the true values than estimates made with LS, especially for poorly determined problems. When the deviation is in pressure only, TLS and LS results are comparable. However, in the presence of deviations in time in addition to pressure, the performance of TLS algorithms is substantially better. We, therefore, expect that our technique will provide more accurate estimation of reservoir parameters, allowing for better forecasting of reservoir performance.
A Fully Coupled Three-Phase Model for Capillary Pressure and Relative Permeability for Implicit Compositional Reservoir Simulation
Authors: Hustad Odd Steve , Browning David John 
Published: August 2010

Summary
A coupled formulation for three-phase capillary pressure and relative permeability for implicit compositional reservoir simulation is presented. The formulation incorporates primary, secondary, and tertiary saturation functions. Hysteresis and miscibility are applied simultaneously to both capillary pressure and relative permeability. Two alternative three-phase capillary pressure formulations are presented: the first as described by Hustad (2002) and the second that incorporates six representative two-phase capillary pressures in a saturation-weighting scheme. Consistency is ensured for all three two-phase boundary conditions through the application of two-phase data and normalized saturations.
Simulation examples of water-alternating-gas (WAG) injection are presented for water-wet and mixed-wet saturation functions. 1D homogeneous and 2D and 3D heterogeneous examples are employed to demonstrate some model features and performance.