bluuebunny/crossref_metadata_2025_split huggingface dataset:
On the Use of Scattering Kernels to Calculate Ice Cloud Bulk Optical Properties
Authors: Liu Xiaodong , Ding Shouguo , Bi Lei , Yang Ping 
Published: August 2011

AbstractNonspherical ice crystal optical properties are of fundamental importance to atmospheric radiative transfer through an ice cloud and the remote sensing of its properties. In practice, the optical properties of individual ice crystals need to be integrated over particle size distributions to derive the bulk optical properties of ice clouds. Given a particle size distribution represented in terms of size bins, the conventional approach uses the microphysical and optical properties of ice crystals at the bin centers as approximations to the bin-averaged values. However, errors are incurred when the size bins are large. To reduce the potential errors, a kernel technique is utilized to calculate the bulk optical properties of ice clouds by computing the bin-averaged values instead of using the bin-center values. Comparisons between the solutions based on the conventional method and the kernel technique for different numbers of size bins from in situ measurements demonstrate that the results computed from the kernel technique are more accurate. The present study illustrates that, for a given size distribution, 40 or more size bins should be used to calculate the bulk optical properties of ice clouds by the conventional method. Although the accuracy of bulk-scattering properties can be improved by using fine bin resolutions in the single-scattering property computation, the advantage of using a precomputed database of scattering kernels allows efficient computation of ice cloud bulk optical properties without losing the accuracy.
A First Look at the Structure of the Wave Pouch during the 2009 PREDICT–GRIP Dry Runs over the Atlantic
Authors: Wang Zhuo , Montgomery Michael T. , Fritz Cody 
Published: August 2011

In support of the National Science Foundation Pre-Depression Investigation of Cloud-systems in the tropics (NSF PREDICT) and National Aeronautics and Space Administration Genesis and Rapid Intensification Processes (NASA GRIP) dry run exercises and National Oceanic and Atmospheric Administration Hurricane Intensity Forecast Experiment (NOAA IFEX) during the 2009 hurricane season, a real-time wave-tracking algorithm and corresponding diagnostic analyses based on a recently proposed tropical cyclogenesis model were applied to tropical easterly waves over the Atlantic. The model emphasizes the importance of a Lagrangian recirculation region within a tropical-wave critical layer (the so-called pouch), where persistent deep convection and vorticity aggregation as well as column moistening are favored for tropical cyclogenesis. Distinct scenarios of hybrid wave–vortex evolution are highlighted. It was found that easterly waves without a pouch or with a shallow pouch did not develop. Although not all waves with a deep pouch developed into a tropical storm, a deep wave pouch had formed prior to genesis for all 16 named storms originating from monochromatic easterly waves during the 2008 and 2009 seasons. On the other hand, the diagnosis of two nondeveloping waves with a deep pouch suggests that strong vertical shear or dry air intrusion at the middle–upper levels (where a wave pouch was absent) can disrupt deep convection and suppress storm development. To sum up, this study suggests that a deep wave pouch extending from the midtroposphere (~600–700 hPa) down to the boundary layer is a necessary condition for tropical cyclone formation within an easterly wave. It is hypothesized also that a deep wave pouch together with other large-scale favorable conditions provides a sufficient condition for sustained convection and tropical cyclone formation. This hypothesized sufficient condition requires further testing and will be pursued in future work.
Coordinate loss of a microRNA and protein-coding gene cooperate in the pathogenesis of 5q− syndrome
Authors: Kumar Madhu S. , Narla Anupama , Nonami Atsushi , Mullally Ann , Dimitrova Nadya , Ball Brian , McAuley J. Randall , Poveromo Luke , Kutok Jeffrey L. , Galili Naomi , Raza Azra , Attar Eyal , Gilliland D. Gary , Jacks Tyler , Ebert Benjamin L. 
Published: August 2011

Abstract
Large chromosomal deletions are among the most common molecular abnormalities in cancer, yet the identification of relevant genes has proven difficult. The 5q− syndrome, a subtype of myelodysplastic syndrome (MDS), is a chromosomal deletion syndrome characterized by anemia and thrombocytosis. Although we have previously shown that hemizygous loss of RPS14 recapitulates the failed erythroid differentiation seen in 5q− syndrome, it does not affect thrombocytosis. Here we show that a microRNA located in the common deletion region of 5q− syndrome, miR-145, affects megakaryocyte and erythroid differentiation. We find that miR-145 functions through repression of Fli-1, a megakaryocyte and erythroid regulatory transcription factor. Patients with del(5q) MDS have decreased expression of miR-145 and increased expression of Fli-1. Overexpression of miR-145 or inhibition of Fli-1 decreases the production of megakaryocytic cells relative to erythroid cells, whereas inhibition of miR-145 or overexpression of Fli-1 has a reciprocal effect. Moreover, combined loss of miR-145 and RPS14 cooperates to alter erythroid-megakaryocytic differentiation in a manner similar to the 5q− syndrome. Taken together, these findings demonstrate that coordinate deletion of a miRNA and a protein-coding gene contributes to the phenotype of a human malignancy, the 5q− syndrome.
Fine-tuning nucleophosmin in macrophage differentiation and activation
Authors: Guery Leslie , Benikhlef Naïma , Gautier Thomas , Paul Catherine , Jego Gaetan , Dufour Erick , Jacquel Arnaud , Cally Radj , Manoury Bénédicte , Vanden Berghe Tom , Vandenabeele Peter , Droin Nathalie , Solary Eric 
Published: August 2011

Abstract
M-CSF–driven differentiation of peripheral blood monocytes is one of the sources of tissue macrophages. In humans and mice, the differentiation process involves the activation of caspases that cleave a limited number of proteins. One of these proteins is nucleophosmin (NPM1), a multifunctional and ubiquitous protein. Here, we show that caspases activated in monocytes exposed to M-CSF cleave NPM1 at D213 to generate a 30-kDa N-terminal fragment. The protein is further cleaved into a 20-kDa fragment, which involves cathepsin B. NPM1 fragments contribute to the limited motility, migration, and phagocytosis capabilities of resting macrophages. Their activation with lipopolysaccharides inhibits proteolytic processes and restores expression of the full-length protein that negatively regulates the transcription of genes encoding inflammatory cytokines (eg, NPM1 is recruited with NF-κB on the MCP1 gene promoter to decrease its transcription). In mice with heterozygous npm gene deletion, cytokine production in response to lipopolysaccharides, including CXCL1 (KC), MCP1, and MIP2, is dramatically enhanced. These results indicate a dual function of NPM1 in M-CSF–differentiated macrophages. Proteolysis of the protein participates in the establishment of a mature macrophage phenotype. In response to inflammatory stimuli, the full-length protein negatively regulates inflammatory cytokine production.
Addition of rituximab to chemotherapy alone as first-line therapy improves overall survival in elderly patients with mantle cell lymphoma
Authors: Griffiths Robert , Mikhael Joseph , Gleeson Michelle , Danese Mark , Dreyling Martin 
Published: August 2011

Abstract
Clinical trials have demonstrated that rituximab improves overall survival in non-Hodgkin lymphoma (NHL), except in mantle cell lymphoma (MCL). We used Surveillance Epidemiology and End Results (SEER)–Medicare data to compare survival in older MCL patients who began chemotherapy with or without rituximab within 180 days of diagnosis. Patients were followed from diagnosis (January 1999 to December 2005) until death or the end of observation (December 2007). Medicare administrative and claims data were used to identify the date and cause of death and the immunochemotherapy regimen. Of 638 patients, the mean age at diagnosis was 75 years, 75% had stage III/IV disease, 67% had extranodal involvement, and 64% received rituximab. The average length of first-line treatment was 21 weeks, with no difference between the 2 groups (P = .76). Median survival was 27 months for chemotherapy alone, compared with 37 months for chemotherapy plus rituximab (P < .001). In multivariate analysis of 2-year survival, rituximab plus chemotherapy was associated with lower all-cause (hazard ratio [HR] 0.58; 95% confidence interval [CI] 0.41-0.82; P < .01), and cancer-specific (HR 0.56; 95% CI 0.37-0.84; P < .01) mortality. Results were similar when using the entire observation period, propensity score analysis, and limiting chemotherapy to CHOP/CHOP-like. We conclude that first-line chemotherapy including rituximab is associated with significantly improved survival in older patients diagnosed with MCL.
Prognostic implications of the IDH1 synonymous SNP rs11554137 in pediatric and adult AML: a report from the Children's Oncology Group and SWOG
Authors: Ho Phoenix A. , Kopecky Kenneth J. , Alonzo Todd A. , Gerbing Robert B. , Miller Kristen L. , Kuhn Julia , Zeng Rong , Ries Rhonda E. , Raimondi Susana C. , Hirsch Betsy A. , Oehler Vivian , Hurwitz Craig A. , Franklin Janet L. , Gamis Alan S. , Petersdorf Stephen H. , Anderson Jeanne E. , Godwin John E. , Reaman Gregory H. , Willman Cheryl L. , Bernstein Irwin D. , Radich Jerald P. , Appelbaum Frederick R. , Stirewalt Derek L. , Meshinchi Soheil 
Published: August 2011

Abstract
IDH1 SNP rs11554137 was recently reported in association with poor prognosis in normal karyotype adult acute myeloid leukemia (AML). We aimed to determine the prevalence, clinical associations, and prognostic significance of SNP rs11554137 in unselected pediatric and adult AML patients. Diagnostic marrow specimens from 527 AML patients treated on the pediatric trial Children's Oncology Group-AAML03P1 (N = 253) or adult SWOG trials (N = 274) were analyzed for the presence of the SNP. SNP rs11554137 was present in 11% of all patients. SNP status had no prognostic impact on survival in pediatric patients. In adult AML, overall survival for SNP-positive patients was 10% versus 18% for SNP-negative patients (P = .44). Among the 142 adults who achieved complete remission, 5-year relapse-free survival was significantly worse for SNP-positive patients (0% vs 25%, P = .0014). However, among adults with normal cytogenetics, FLT3/ITD was present in 90% of SNP-positive patients versus 59% of SNP-negative patients (P = .0053). In multivariate analysis, adjusting for the effects of age, cytogenetics, and FLT3/ITD, the independent prognostic effect of SNP positivity was not statistically significant (hazard ratio = 1.72, P = .18). The clinical profile of SNP-positive patients suggests that SNP rs11554137 may have biologic effects that bear further investigation. The clinical trials in this study are registered at http://www.clinicaltrials.gov as #NCT000707174 and #NCT00899171.
De novo deletion in MECP2 in a monozygotic twin pair: a case report
Authors: Mittal Kirti , Kabra Madhulika , Juyal Ramesh , BK Thelma 
Published: August 2011

AbstractBackgroundRett syndrome (RTT) is a severe, progressive, neurodevelopmental disorder predominantly observed in females that leads to intellectual disability. Mutations and gross rearrangements inMECP2account for a large proportion of cases with RTT. A limited number of twin pairs with RTT have also been reported in literature.Case PresentationWe investigated 13 year old, monozygotic twin females with RTT and some noticeable differences in development using a combinatorial approach of sequencing and Taqman assay. Monozygosity status of the twins was confirmed by informative microsatellite markers.ConclusionsThe twins shared ade novodeletion in exon 3 in the MBD domain ofMECP2. To the best of our knowledge, this is only the second report of genetic analysis of a monozygotic twin pair.
Resveratrol Inhibits Cdk5 Activity through Regulation of p35 Expression
Authors: Utreras Elias , Terse Anita , Keller Jason , ladarola Michael J , Kulkarni Ashok B 
Published: August 2011

Background: We have previously reported that cyclin-dependent kinase 5 (Cdk5) participates in the regulation of nociceptive signaling. Through activation of the ERK1/2 pathway, Tumor Necrosis Factor-α (TNF-α) induces expression of Egr-1. This results in the sustained and robust expression of p35, a coactivator of Cdk5, in PC12 cells, thereby increasing Cdk5 kinase activity. The aim of our present study was to test whether resveratrol, a polyphenolic compound with known analgesic activity, can regulate Cdk5/p35 activity. Results: Here we used a cell-based assay in which a p35 promoter-luciferase construct was stably transfected in PC12 cells. Our studies demonstrate that resveratrol inhibits p35 promoter activity and also blocks the TNF-α mediated increase in Cdk5 activity in PC12 cells. Resveratrol also inhibits p35 expression and blocks the TNF-α mediated increase in Cdk5 activity in DRG neurons. In the presence of resveratrol, the MEK inhibitor decreased p35 promoter activity, whereas the inhibitors of p38 MAPK, JNK and NF- κB increased p35 promoter activity, indicating that these pathways regulate p35 expression differently. The TNF-α-mediated increase in Egr-1 expression was decreased by resveratrol treatment with a concomitant reduction in p35 expression and protein levels, resulting in reduced Cdk5 kinase activity. Conclusions: We demonstrate here that resveratrol regulates p35 promoter activity in PC12 cells and DRG neurons. Most importantly, resveratrol blocks the TNF-α-mediated increase in p35 promoter activity, thereby reducing p35 expression and subsequent Cdk5 kinase activity. This new molecular mechanism adds to the known analgesic effects of resveratrol and confirms the need for identifying new analgesics based on their ability to inhibit Cdk5 activity for effective treatment of pain.
Impact of education and training on type of care provided by community-based breastfeeding counselors: a cross-sectional study
Authors: Sullivan Elizabeth M , Bignell Whitney E , Andrianos Anne , Anderson Alex K 
Published: August 2011

Abstract

Background
Studies using community-based breastfeeding counselors (CBBCs) have repeatedly shown positive impact on breastfeeding initiation, exclusivity and duration, particularly among low-income mothers. To date, there has not been a comprehensive study to determine the impact of CBBC attributes such as educational background and training, on the type of care that CBBCs provide.

Methods
This was a cross-sectional study of a convenience sample of CBBCs to ascertain the influence of counselor education and type of training on type of support and proficiency of CBBCs in communities across the United States. Invitations to participate in this online survey of CBBCs were e-mailed to program coordinators of the Special Supplemental Nutrition Program for Women, Infants, and Children (WIC), La Leche League, and other community-based health organizations, who in turn invited and encouraged their CBBCs to participate. Descriptive analysis was used to describe participants (N = 847), while bivariate analysis using χ2 test was used to examine the differences between CBBC education, training received and breastfeeding support skills used. Multivariate logistic regression was used to assess the independent determinants of specific breastfeeding support skills.

Results
The major findings from the research indicate that overall, educational attainment of CBBCs is not a significant predictor for the curriculum used in their training and type of support skills used during counseling sessions, but initial training duration was positively associated with the use of many breastfeeding support skills. Another major influence of counselor support to clients is the type of continuing education they receive after their initial training, with higher likelihood of use of desirable support skills associated with counselors continuing their breastfeeding education at conferences or trainings away from their job sites.

Conclusions
Our results show that different programs use different training curricula to train their CBBCs varying in duration and content. Counselor education is not a significant predictor of the type of training they receive. Continuing breastfeeding education is a significant determinant of type of counseling techniques used with clients. Further research is therefore needed to critically examine the content of the various training curricula of CBBC programs. This may show a need for a standardized training curriculum for all CBBC programs worldwide to make CBBCs more proficient and efficient, ensuring successful and optimum breastfeeding experiences for mothers and their newborns.
Precision of the transpulmonary thermodilution measurements
Authors: Monnet Xavier , Persichini Romain , Ktari Mariem , Jozwiak Mathieu , Richard Christian , Teboul Jean-Louis 
Published: August 2011

Abstract
Introduction
We wanted to determine the number of cold bolus injections that are necessary for achieving an acceptable level of precision for measuring cardiac index (CI), indexed global end-diastolic volume (GEDVi) and indexed extravascular lung water (EVLWi) by transpulmonary thermodilution.

Methods
We included 91 hemodynamically stable patients (age 59 (25% to 75% interquartile range: 39 to 79) years, simplified acute physiologic score (SAPS)II 59 (53 to 65), 56% under norepinephrine) who were monitored by a PiCCO2 device. We performed five successive cold saline (15 mL, 6°C) injections and recorded the measurements of CI, GEDVi and EVLWi.

Results
Considering five boluses, the coefficient of variation (CV, calculated as standard deviation divided by the mean of the five measurements) was 7 (5 to 11)%, 7 (5 to 12)% and 7 (6 to 12)% for CI, GEDVi and EVLWi, respectively. If the results of two bolus injections were averaged, the precision (2 × CV/√ number of boluses) was 10 (7 to 15)%, 10 (7 to 17)% and 8 (7 to 14)% for CI, GEDVi and EVLWi, respectively. If the results of three bolus injections were averaged, the precision dropped below 10%, that is, the cut-off that is generally considered as acceptable (8 (6 to 12)%, 8 (6 to 14)% and 8 (7 to 14)% for CI, GEDVi and EVLWi, respectively). If two injections were performed, the least significant change, that is, the minimal change in value that could be trusted to be significant, was 14 (10 to 21)%, 14 (10 to 24)% and 14 (11 to 23)% for CI, GEDVi and EVLWi, respectively. If three injections were performed, the least significant change was 12 (8 to 17)%, 12 (8 to 19)% and 12 (9 to 19)% for CI, GEDVi and EVLWi, respectively, that is, below the 15% cut-off that is usually considered as clinically relevant.

Conclusions
These results support the injection of at least three cold boluses for obtaining an acceptable precision when transpulmonary thermodilution is used for measuring CI, GEDVi and EVLWi.
Maternal KIR and fetal HLA-C: a fine balance
Authors: Chazara Olympe , Xiong Shiqiu , Moffett Ashley 
Published: August 2011

ABSTRACTNK cell effector function is regulated by a range of activating and inhibitory receptors, and many of their known ligands are MHC class I molecules. Human NK receptors encoded by the Killer immunoglobulin-like receptor (KIR) gene family recognize polymorphic HLA-C as well as some HLA-A and HLA-B molecules. KIRs are expressed by uterine NK (uNK) cells, which are distinctive NK cells directly in contact with the invading fetal placental cells that transform the uterine arteries during the first trimester. Trophoblast cells express both maternal and paternal HLA-C allotypes and can therefore potentially interact with KIRs expressed by uNK. Therefore, allorecognition of paternal HLA-C by maternal KIR might influence trophoblast invasion and vascular remodeling, with subsequent effects on placental development and the outcome of pregnancy. We discuss here the studies relating to KIR/HLA-C interactions with an emphasis on how these function during pregnancy to regulate placentation.
Aquaporin 9 phosphorylation mediates membrane localization and neutrophil polarization
Authors: Karlsson Thommie , Glogauer Michael , Ellen Richard P , Loitto Vesa-Matti , Magnusson Karl-Eric , Magalhães Marco A O 
Published: August 2011

ABSTRACTNeutrophils are of prime importance in the host innate defense against invading microorganisms by using two primary mechanisms—locomotion toward and phagocytosis of the prey. Recent research points to pivotal roles for water channels known as AQPs in cell motility. Here, we focused on the role of AQP9 in chemoattractant-induced polarization and migration of primary mouse neutrophils and neutrophil-like HL60 cells. We found that AQP9 is phosphorylated downstream of fMLFR or PMA stimulation in primary human neutrophils. The dynamics of AQP9 were assessed using GFP-tagged AQP9 constructs and other fluorescent markers through various live-cell imaging techniques. Expression of WT or the phosphomimic S11D AQP9 changed cell volume regulation as a response to hyperosmotic changes and enhanced neutrophil polarization and chemotaxis. WT AQP9 and S11D AQP9 displayed a very dynamic distribution at the cell membrane, whereas the phosphorylation-deficient S11A AQP9 failed to localize to the plasma membrane. Furthermore, we found that Rac1 regulated the translocation of AQP9 to the plasma membrane. Our results show that AQP9 plays an active role in neutrophil volume regulation and migration. The display of AQP9 at the plasma membrane depends on AQP9 phosphorylation, which appeared to be regulated through a Rac1-dependent pathway.
Surface-Enhanced Raman Scattering from Photoreduced Ag Nanoaggregates on an Optically Trapped Single Bacterium
Authors: Kitahama Yasutaka , Itoh Tamitake , Ishido Tomomi , Hirano Ken , Ishikawa Mitsuru 
Published: August 2011

Abstract
A single bacterium in an aqueous Ag nitrate solution was optically trapped by focusing a near-infrared laser beam, and then Ag nanoaggregates were formed on the bacterium by focusing a green laser beam at the bacterium. We obtained surface-enhanced Raman scattering signals from a focal area on a single bacterium.
DFT Analysis of Catalytic Urethanation
Authors: Hatanaka Masashi 
Published: August 2011

Abstract
Reaction profiles for catalytic urethanation are analyzed by DFT calculations. A well-known basic catalyst 1,4-diazabicyclo[2.2.2]octane (DABCO) activates alcohol molecules and accelerates the direct addition toward isocyanates. Self-catalysis by alcohols is also characterized as an accelerated direct addition.