bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optical and molecular techniques to identify tumor margins within the larynx
Authors: Hughes Owain R. , Stone Nicholas , Kraft Marcel , Arens Christoph , Birchall Martin A. 
Published: January 2010

AbstractFailure to remove tumor cells from the larynx significantly increases the risk of local recurrence following surgical excision. Healthy tissue must be preserved to optimize long‐term vocal and swallowing function. It is essential to accurately distinguish between healthy mucosa, dysplasia, and invasive carcinoma. Optical and molecular examining technologies have been developed to improve tumor margin identification in vivo. We aimed to review the efficacy of these technologies. Published articles were identified using MEDLINE, EMBASE, and Cochrane central register of controlled trials (CENTRAL). Randomized clinical trials are required to establish the benefit to patients and cost to the health service of using 5‐aminolevulinic acid (ALA)–induced fluorescent imaging, contact endoscopy, and optical coherence tomography (OCT). Furthermore, primary research is required to validate other techniques, such as confocal endomicroscopy and Raman spectroscopy, and to develop their clinical applications in the larynx. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Usefulness of intraoperative parathyroid hormone measurements in patients with renal hyperparathyroidism
Authors: Freriks Kim , Hermus Ad R.M.M. , de Sévaux Ruud G.L. , Bonenkamp Han J. , Biert Jan , den Heijer Martin , Sweep Fred C.G.J. , van Hamersvelt Henk W. 
Published: January 2010

AbstractBackgroundIn renal hyperparathyroidism, it remains unclear whether intraoperative parathyroid hormone (PTH) measurements can predict postoperative outcome and guide the extent of surgical exploration.MethodsIn 42 parathyroidectomies for renal hyperparathyroidism, we analyzed the predictive value of the Miami Criterion of 50% intraoperative PTH decrease. We used receiver operating characteristic (ROC) curves to find the criterion with the best diagnostic performance. We also investigated whether the whole PTH assay improved accuracy.ResultsTwenty‐six operations (62%) resulted in normal postoperative PTH. With the Miami Criterion, cure was predicted with a sensitivity of 95% and specificity of only 8%. Specificity could be improved to 50% using a 70% PTH decrease as cut‐off level. The whole PTH assay did not improve accuracy.ConclusionPrediction of cure after parathyroidectomy for renal hyperparathyroidism might be improved with a criterion of 70% PTH decrease 10 minutes after excision of all parathyroid glands. Prospective analysis needs to validate this new criterion. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Predicting the pattern of regional metastases from cutaneous squamous cell carcinoma of the head and neck based on location of the primary
Authors: Ebrahimi Ardalan , Moncrieff Marc D. , Clark Jonathan R. , Shannon Kerwin F. , Gao Kan , Milross Christopher G. , O'Brien Christopher J. 
Published: January 2010

AbstractBackgroundWe aimed to analyze the distribution of regional nodal metastases according to primary tumor location in patients with cutaneous squamous cell carcinoma of the head and neck (SCCHN).MethodsAnalysis of 295 neck dissections performed for patients with clinically evident regional metastases from cutaneous SCCHN between 1987 and 2009.ResultsLevel I involvement in the absence of level II or III only occurred in patients with facial primaries. In patients with clear nodes in level II–III, the risk of level IV–V involvement was 0.0% for external ear primaries, 2.7% for face and anterior scalp, and 15.8% for posterior scalp and neck.ConclusionIn patients undergoing parotidectomy for metastatic cutaneous SCCHN with a clinically negative neck, the results of this study support selective neck dissection including level I–III for facial primaries, level II–III for anterior scalp and external ear primaries, and levels II–V for posterior scalp and neck primaries. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Proliferative activity and diagnostic delay in oral cancer
Authors: Seoane Juan , Pita‐Fernández Salvador , Gómez Iria , Vazquez Inés , López‐Cedrún José Luis , De Agustin Domingo , Varela‐Centelles Pablo 
Published: January 2010

AbstractBackgroundTumor stage may relate to the chronology of neoplasm growth and has been used as an outcome variable when studying diagnostic delay in oral cancer. However, tumor growth rate may act as a confounding factor.MethodsWe reviewed a total of 63 incident cases of oral cancer. The variables considered for the study included age, sex, smoking history, tumor site, TNM stage, Ki‐67 score, and diagnostic delay.ResultsSignificant differences between survivors and exitus were found in terms of tumor stage at diagnosis (I–II vs III–IV), sex, and Ki‐67 scores. When the analysis was adjusted for tumor stage at diagnosis (I–II vs III–IV), proliferative activity resulted to be an independent prognostic factor for survival, whereas diagnostic delay did not influence survival.ConclusionThese results seem to suggest that survival from oral cancer is affected more by the biology of the cancer (rapid tumor growth) than by diagnostic delay. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Additional ent‐Kaurane Diterpenoids from Rubus corchorifolius L. f.
Authors: Chen Xue‐Xiang , Zhou Shuang‐De , Ou Yang‐Wen , Xiao Su‐Yao , Lin Wang‐Huang , Cao Yong , Zhang Min , Zhao Li‐Chao , Li Long‐Fang 
Published: January 2010

AbstractA further chemical investigation of the plant Rubus corchorifolius L. f., collected in Hunan Province, afforded three new ent‐kauranoids 3–5. Their structures were elucidated by various spectroscopic methods.
A Novel and Expedient Synthesis of 7‐Pyrimidinylpyrimido[4,5‐d]pyrimidinones
Authors: Mohammadizadeh Mohammad R. , Bahramzadeh Mojtaba , Mohammadi Ali A. , Karimi Ali R. 
Published: January 2010

AbstractA novel and efficient procedure for the synthesis of new 7‐pyrimidinylpyrimido[4,5‐d]pyrimidinone derivatives was elaboratedviathe base‐promoted cyclodimerization reaction of 5‐[(dimethylamino)methylidene]‐6‐iminopyrimidine‐2,4(1H,3H)‐dione hydrochlorides. In an analogous manner, a 2‐thioxo analog was prepared starting with the corresponding 2‐thioxopyrimidin‐4‐one.
Two New Metabolites, Epoxydine A and B, from Phoma sp.
Authors: Qin Song , Hussain Hidayat , Schulz Barbara , Draeger Siegfried , Krohn Karsten 
Published: January 2010

AbstractThe new compounds, epoxydines A and B (1 and 2, resp.), along with the known and related metabolites, 3–6, were isolated from the fungal endophyte Phoma sp. The structures of the new compounds were elucidated by detailed spectroscopic analysis, and the relative configuration of 1 was confirmed by ROESY experiments. Preliminary studies indicated that compounds 2–5 possess good antibacterial, antifungal, and algicidal properties. Similarly, compound 1 showed antifungal and algicidal, and compound 6 antibacterial and algicidal properties.
Photoannelation Reactions of 3‐(Alk‐1‐ynyl)cyclohept‐2‐en‐1‐ones
Authors: Vallée M. Robert J. , Inhülsen Inga , Margaretha Paul 
Published: January 2010

AbstractIrradiation (350 nm) of the newly synthesized 3‐(alk‐1‐ynyl)cyclohept‐2‐en‐1‐ones 1 and 2 leads to the selective formation of tricyclic head‐to‐head dimers. In the presence of 2,3‐dimethylbuta‐1,3‐diene, the (monocyclic) enone 1 affords trans‐fused 7‐alkynyl‐bicyclo[5.2.0]nonan‐2‐ones as major photoproducts, whereas photocycloaddition of benzocyclohept‐5‐en‐7‐one 2 to the same diene gives preferentially the eight‐membered cyclic allene 16 via ‘end‐to‐end’ cyclization of the intermediate allyl‐propargyl biradical 22. On contact with acid, cycloocta‐1,2,5‐triene 16 isomerizes to cycloocta‐1,3,5‐triene 18.
Neolignans from the Leaves of Casearia sylvestrisSwartz
Authors: Wang Wei , Ali Zulfiqar , Li Xing‐Cong , Khan Ikhlas A. 
Published: January 2010

AbstractSix new neolignans, casearialignans A–F (1–6, resp.) and one known lignan syringaresinol‐β‐D‐glucoside were isolated from the leaves of Casearia sylvestris. Their structures were determined on the basis of 1D‐ and 2D‐NMR, and HR‐ESI‐MS analyses. The relative and absolute configurations were determined by the value of the coupling constants and CD spectral analysis, respectively.
Alternative Synthesis of 2‐Hydroxy‐3,5,5‐trimethylcyclopent‐2‐en‐1‐one
Authors: Chapuis Christian , Saint‐Léger Christine 
Published: January 2010

AbstractThe 2‐hydroxy‐3,5,5‐trimethylcyclopent‐2‐en‐1‐one (1) was synthesized in 42% yield by rearrangement of epoxy ketone 10 on treatment with BF3⋅Et2O under anhydrous conditions. Intermediate 10 was available from the known enone 8, either via direct epoxidation (60% H2O2, NaOH, MeOH; yield 50%), or via reduction to the corresponding allylic alcohol 14 (LiAlH4, THF), followed by epoxidation ([VO(acac)2], tBuOOH) and reoxidation under Swern conditions, in 37% total yield.
Experimental and Theoretical Conformational Analysis of 5‐Benzylimidazolidin‐4‐one Derivatives – a ‘Playground’ for Studying Dispersion Interactions and a ‘Windshield‐Wiper’ Effect in Organocatalysis
Authors: Seebach Dieter , Grošelj Uroš , Schweizer W. Bernd , Grimme Stefan , Mück‐Lichtenfeld Christian 
Published: January 2010

AbstractThe PF6 salts of 5‐benzyl‐1‐isopropylidene‐ and 5‐benzyl‐1‐cinnamylidene‐3‐methylimidazolidin‐4‐ones 1 (Scheme) with various substituents in the 2‐position have been prepared, and single crystals suitable for X‐ray structure determination have been obtained of 14 such compounds, i.e., 2–10 and 12–16 (Figs. 2–5). In nine of the structures, the Ph ring of the benzyl group resides above the heterocycle, in contact with the cis‐substituent at C(2) (staggered conformation A; Figs. 1–3); in three structures, the Ph ring lies above the iminium π‐plane (staggered conformation B; Figs. 1 and 4); in two structures, the benzylic CC bond has an eclipsing conformation (C; Figs. 1 and 5) which places the Ph ring simultaneously at a maximum distance with its neighbors, the CO group, the NC‐π‐system, and the cis‐substituent at C(2) of the heterocycle. It is suggested by a qualitative conformational analysis (Fig. 6) that the three staggered conformations of the benzylic CC bond are all subject to unfavorable steric interactions, so that the eclipsing conformation may be a kind of ‘escape’. State‐of‐the‐art quantum‐chemical methods, with large AO basic sets (near the limit) for the single‐point calculations, were used to compute the structures of seven of the 14 iminium ions, i.e., 3, 4/12, 5–7, 13, and 16 (Table) in the two staggered conformations, A and B, with the benzylic Ph group above the ring and above the iminium π‐system, respectively. In all cases, the more stable computed conformer (‘isolated‐molecule’ structure) corresponds to the one present in the crystal (overlay in Fig. 7). The energy differences are small (≤2 kcal/mol) which, together with the result of a potential‐curve calculation for the rotation around the benzylic CC bond of one of the structures, 16 (Fig. 8), suggests that the benzyl group is more or less freely rotating at ambident temperatures. The importance of intramolecular London dispersion (benzene ring in ‘contact’ with the cis‐substituent in conformation A) for DFT and other quantum‐chemical computations is demonstrated; the benzyl‐imidazolidinones 1 appear to be ideal systems for detecting dispersion contributions between a benzene ring and alkyl or aryl CH groups. Enylidene ions of the type studied herein are the reactive intermediates of enantioselective organocatalytic conjugate additions, Diels–Alder reactions, and many other transformations involving α,β‐unsaturated carbonyl compounds. Our experimental and theoretical results are discussed in view of the performance of 5‐benzyl‐imidazolidinones as enantioselective catalysts.
Applications of the Chiral Auxiliaries DIOZ and TRIOZ for Conjugate Additions and Comparison with Other Auxiliaries
Authors: Sprecher Hanspeter , Pletscher Stefan , Möri Manuel , Marti Roger , Gaul Christoph , Patora‐Komisarska Krystyna , Otchertianova Ekatarina , Beck Albert K. , Seebach Dieter 
Published: January 2010

AbstractA number of N‐acryloyl‐, N‐crotonoyl‐, N‐(3,3,3‐trifluorocrotonoyl)‐, N‐cinnamoyl‐, and N‐(3‐nitroacryloyl)‐4‐isopropyl‐ or ‐4‐phenyl‐oxazolidin‐2‐ones with geminal diphenyl substitution, i.e., 7–15, have been prepared and used for conjugate additions of organocuprate reagents (Me, iPr, Ph, 4‐MeOPh) in the β‐carbonyl (Table 2) and in the α‐carbonyl position (NO2‐derivative 11 in Scheme 3). The yields and diastereoselectivities are compared with previously tested enoyl‐oxazolidinones (Table 2). Highest diastereoselectivities (>90%) are always observed with the 4‐Ph derivatives (Hruby effect). Nitroacryloyl‐oxazolidinones and a corresponding phenylmenthol ester undergo less diastereoselective additions (Scheme 3). A 3‐(1‐methylethyl)‐5,5‐diphenyloxazolidin‐2‐one (DIOZ)‐derived Li2‐enolate‐nitronate was also tested for α‐carbonyl alkylation (Scheme 4). The X‐ray crystal structures of three acryloyl‐oxazolidinones and of four adducts are described (Tables 1 and 3), and they serve for configurational assignments and description of the stereochemical courses of the additions and alkylation. Possible applications of the nitro compounds for the preparation of β2‐amino acids are discussed (Scheme 2).
Fibromyalgia: a complex syndrome requiring a multidisciplinary approach
Authors: Spaeth Michael , Briley Mike 
Published: January 2010

AbstractFibromyalgia is a pain syndrome which is not due to tissue damage or inflammation and is thus fundamentally different from rheumatic disorders and many other pain conditions. In addition to widespread pain it is associated with a range of other symptoms such as sleep disturbance, fatigue, cognitive disturbance, stiffness and depressive symptoms. A number of multidisciplinary therapeutic programmes involving education, exercise and cognitive therapy have been shown to be effective in bringing relief. The various medications that are currently being developed for the treatment of fibromyalgia are based on different mechanistic approaches. In particular, serotonin noradrenaline reuptake inhibitors (SNRI) such as duloxetine and milnacipran and α2‐δ receptor ligands such as pregabalin have been shown, in a variety of placebo‐controlled studies, to bring significant relief from pain and other symptoms. The complex symptomatology of fibromyalgia will, however, continue to require a multidisciplinary approach including education and exercise in addition to drug therapy to achieve the most efficient management of fibromyalgia. Copyright © 2009 John Wiley & Sons, Ltd.
Effect of a small molecule BCL‐2 inhibitor on immune function and use with a recombinant vaccine
Authors: Farsaci Benedetto , Sabzevari Helen , Higgins Jack P. , Di Bari Maria Giovanna , Takai Shinji , Schlom Jeffrey , Hodge James W. 
Published: January 2010

AbstractSmall molecule BCL‐2 inhibitors are being examined as monotherapy in phase I/II clinical trials for several types of tumors. However, few data are available about the effect of BCL‐2 inhibitors on immune function. The aims of our study were to investigate the effect of a small molecule BCL‐2 inhibitor on immune function and determine the most effective way of combining this inhibitor with a recombinant vaccine to treat tumors. The in vitro effect of the pan‐BCL‐2 inhibitor GX15‐070 was assessed in mouse CD8 T lymphocytes at 2 different stages of activation as well as regulatory T lymphocytes (Treg). The in vivo effect of GX15‐070 after recombinant vaccinia/fowlpox CEA‐TRICOM vaccination was analyzed in tumor‐infiltrating lymphocytes, and in splenocytes of mice bearing subcutaneous tumors. The therapeutic efficacy of such sequential therapy was measured as a reduction of pulmonary tumor nodules. Activated mature CD8 T lymphocytes were more resistant to GX15‐070 as compared to early‐activated cells. Treg function was significantly decreased after treatment with the BCL‐2 inhibitor. In vivo, GX15‐070 was given after vaccination so as to not negatively impact the induction of vaccine‐mediated immunity, resulting in increased intratumoral activated CD8:Treg ratio and significant reduction of pulmonary tumor nodules. Our study is the first to show the effect of a small molecule BCL‐2 inhibitor on the immune system and following a vaccine. It is also the first to demonstrate the efficacy of this sequence in reducing tumors in mouse models, providing a rationale for the design of combinational clinical studies.
Retracted: Aurora kinase inhibitor AZD1152 negatively affects the growth and survival of HTLV‐1‐infected T lymphocytes in vitro
Authors: Tomita Mariko , Tanaka Yuetsu , Mori Naoki 
Published: January 2010

AbstractRetraction: The following article has been retracted through agreement between the first author and several coauthors, the journal Editor‐in‐Chief, Peter Lichter, and John Wiley & Sons, Ltd.: Tomita, M., Tanaka, Y. and Mori, N. (2010), Aurora kinase inhibitor AZD1152 negatively affects the growth and survival of HTLV‐1‐infected T lymphocytes in vitro. Int J Cancer, 127:1584–1594, published online on 20 JAN 2010 in Wiley Online Library (www.onlinelibrary.wiley.com). After an investigation the retraction has been agreed due to inappropriate duplication of images and overlap with other published work.
Macroscopic morphologic subtypes of laterally spreading colorectal tumors showing distinct molecular alterations
Authors: Sugimoto Takafumi , Ohta Miki , Ikenoue Tsuneo , Yamada Atsuo , Tada Motohisa , Fujishiro Mitsuhiro , Ogura Keiji , Yamaji Yutaka , Okamoto Makoto , Kanai Fumihiko , Kawabe Takao , Omata Masao 
Published: January 2010

AbstractRecent advances in colonoscopic techniques have resulted in more frequent detection of superficial‐type colorectal tumors, that is, laterally spreading tumors (LSTs), although little is known about the characteristic clinical features and genetic alterations of LSTs. To elucidate the molecular characteristics of LSTs, genetic alterations in the KRAS, BRAF and PIK3CA genes and abnormal expression of the p53, β‐catenin and MYC proteins were analyzed using direct DNA sequencing and immunohistochemistry for 50 protruded‐type tumors (Protruded), 35 granular‐type LSTs (LST‐G) and 19 nongranular‐type LSTs (LST‐NG). In addition, loss of heterozygosity (LOH) close to the adenomatous polyposis coli (APC) gene (5q21) was examined in these tumors. In univariate analyses, significant differences were noted in the percentages with KRAS mutations (Protruded, LST‐G, LST‐NG = 30.0%, 54.3%, 21.1%, respectively, p = 0.0156), nuclear accumulation of β‐catenin (Protruded, LST‐G, LST‐NG = 50.0%, 37.1%, 68.4%, respectively, p = 0.0267), expression of MYC (Protruded, LST‐G, LST‐NG = 26.0%, 17.1%, 42.1%, respectively, p = 0.0456) and LOH at the APC gene locus (Protruded, LST‐G, LST‐NG = 22.0%, 20.0%, 47.4%, respectively, p = 0.0302). Multivariate analysis demonstrated that the macroscopic subtype of LST was significantly associated with KRAS mutation (for LST‐NG: odds ratio [OR] 0.23, 95% CI 0.06–0.90) and nuclear accumulation of β‐catenin (for LST‐NG: OR 4.05, 95% CI 1.11–14.8). Our data revealed that the 2 subtypes of LST have different molecular characteristics, suggesting that 2 or more different molecular mechanisms result in colorectal tumors with a similar growth pattern.
Determining the protein drug release characteristics and cell adhesion to a PLLA or PLGA biodegradable polymer membrane
Authors: Burns Sarah A. , Hard Robert , Hicks Wesley L. , Bright Frank V. , Cohan David , Sigurdson Lynn , Gardella Joseph A. 
Published: January 2010

AbstractBiodegradable polymers are of interest for developing controlled protein drug delivery platforms. In this study, two poly (α‐hydroxy) esters were formulated with Aerosol‐OT, a surfactant stabilizer, to encapsulate the protein keratinocyte growth factor (KGF) for controlled release KGF is involved in a number of crucial biologic processes, most notably epithelial growth and repair. The concentration of KGF that caused a biological response in vitro was determined (optimally 10 ng/mL) and compared with the release of KGF from the two biodegradable polymer membrane formulations. Each polymer formulation released biologically relevant levels, 10 ng/mL of active KGF, although with different times release kinetics. The membrane composed of PLGA/AOT/KGF exhibited a faster release rate of KGF into solution after 120 h of degradation time than the release rate of the PLLA/AOT/KGF matrices. Cell seeding assays showed that both polymer matrices, when formulated with AOT, sustained cell growth. Time of Flight Secondary Ion Mass Spectrometry (ToF‐SIMS) was used to characterize the distribution of AOT and KGF through the polymer membrane. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Effects of parylene‐C photooxidation on serum‐assisted glial and neuronal patterning
Authors: Delivopoulos Evangelos , Murray Alan F. , Curtis John C. 
Published: January 2010

AbstractThe increasing use of patterned neural networks in multielectrode arrays and similar devices drives the constant development and evaluation of new biomaterials. Recently, we presented a promising technique to guide neurons and glia reliably and effectively. Parylene‐C, a common hydrophobic polymer, was photolithographically patterned on silicon oxide (SiO2) and subsequently activated via immersion in serum. In this article, we explore the effects of ultraviolet (UV)‐induced oxidation on parylene's ability to pattern neurons and glia. We exposed parylene‐C stripe patterns to increasing levels of UV radiation and found a dose‐dependent reduction in the total mass of patterned cells, as well as a gradual loss of glial and neuronal conformity to the patterns. In contrast, nonirradiated patterns had superior patterning results and increased presence of cells. The reduced cell adhesion and patterning after the formation of aldehyde and carboxyl groups on UV‐radiated parylene‐C supports our hypothesis that cell adhesion and growth on parylene is facilitated by hydrophobic adsorption of serum proteins. We conclude that unlike other cell patterning schemes, our technique does not rely on photooxidation of the polymer. Nonetheless, the precise control of oxygenated groups on parylene could pave the way for the differential binding of proteins and other molecules on the surface, aiding in the adhesion of alternative cell types. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
In vitroevaluation of cell proliferation and collagen synthesis on titanium following plasma electrolytic oxidation
Authors: Whiteside Paul , Matykina Endzhe , Gough Julie E. , Skeldon Peter , Thompson George E. 
Published: January 2010

AbstractTitania‐based coatings produced by plasma electrolytic oxidation are being investigated as bioactive surfaces for titanium implants. In this study, plasma electrolytic oxidation was performed in calcium‐ and phosphorus‐based electrolytes under DC conditions, resulting in coatings of thickness of ∼8–15 μm. Coating morphologies, microstructures, and compositions were examined by scanning electron microscopy with energy‐dispersive X‐ray analysis, X‐ray diffraction, and electron probe microanalysis. The coatings revealed a cratered morphology, with incorporated calcium and phosphorus species. Proliferation rates of primary human osteoblasts cells on the coatings were up to ∼37% faster than those for uncoated titanium and 316L stainless steel reference materials. Further, the coatings assisted cell adhesion and generation and anchorage of collagen. The amount of collagen was upto ∼2.4 times greater than for the reference substrates. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010