bluuebunny/crossref_metadata_2025_split huggingface dataset:
New low‐frequency platelet glycoprotein polymorphisms associated with neonatal alloimmune thrombocytopenia
Authors: Peterson Julie A. , Gitter Maria L. , Kanack Adam , Curtis Brian , McFarland Janice , Bougie Daniel , Aster Richard 
Published: October 2009

BACKGROUND: Recent reports suggest that maternal immunization against low‐frequency, platelet (PLT)‐specific glycoprotein (GP) polymorphisms is a more common cause of neonatal alloimmune thrombocytopenia (NATP) than previously thought.STUDY DESIGN AND METHODS: Serologic and molecular studies were performed on PLTs and DNA from three families in which an infant was born with apparent NATP not attributable to maternal immunization against known PLT‐specific alloantigens.RESULTS: Antibodies reactive only with paternal PLTs were identified in each mother. In Cases 2 (Kno) and 3 (Nos), but not Case 1 (Sta), antibody recognized paternal GPIIb/IIIa in solid‐phase assays. Unique mutations encoding amino acid substitutions in GPIIb (Case 2) or GPIIIa (Cases 1 and 3) were identified in paternal DNA and in DNA from two of the affected infants. Antibody from all three cases recognized recombinant GPIIIa (Case 1 [Sta] and Case 3 [Nos]) and GPIIb (Case 2, Kno) mutated to contain the polymorphisms identified in the respective fathers. None of 100 unselected normal subjects possessed the paternal mutations. Enzyme‐linked immunosorbent assay and flow cytometric studies suggested that failure of maternal serum from Case 1 (Sta) to react with paternal GPIIIa in solid‐phase assays resulted from use of a monoclonal antibody AP2, for antigen immobilization that competed with the maternal antibody for binding to the Sta epitope.CONCLUSION: NATP in the three cases was caused by maternal immunization against previously unreported, low‐frequency GP polymorphisms. Maternal immunization against low‐frequency PLT‐specific alloantigens should be considered in cases of apparent NATP not resolved by conventional serologic and molecular testing.
Platelet gel for treatment of mucocutaneous lesions related to graft‐versus‐host disease after allogeneic hematopoietic stem cell transplant
Authors: Picardi Alessandra , Lanti Alessandro , Cudillo Laura , Cerretti Raffaella , Dentamaro Teresa , De Angelis Gottardo , Ferraro Angelo , Di Veroli Ambra , Adorno Gaspare , Arcese William 
Published: October 2009

BACKGROUND: Platelet (PLT) gel has been successfully used in tissue regeneration of diabetic/surgical wounds through the releasing of growth factors such as basic fibroblast growth factor and PLT‐derived growth factor. Therefore, the PLT gel could represent a therapeutic tool in treating the deep and painful wounds sometimes occurring during graft‐versus‐host disease (GVHD).STUDY DESIGN AND METHODS: The aim of this study was to verify the efficacy and safety of PLT gel for treating GVHD ulcers. Allogeneic hemocomponents were used to obtain PLT gel with an automated system for the on‐site preparation and application of patient (autologous) or healthy blood donor (allogeneic)‐derived fibrin sealant or PLT‐rich fibrin (Vivostat system, Vivostat A/S). Six patients with multiple lesions involving dermis (Grade I, n = 2), subcutaneous (Grade II, n = 4), or oral mucosa and related to GVHD underwent PLT gel as local therapy.RESULTS: After the second PLT gel application, the pain disappeared in all cases and the granulation tissue was observed in the four patients with Grade II lesions. After a median of eight PLT gel applications (range, 4‐10), five of six patients showed a complete response, while one patient with a partial response died early from multiorgan failure. No side effects were documented.CONCLUSION: These preliminary data show that the PLT gel may be used as a safe and effective tool in the management of mucosal skin lesions related to the GVHD.
Characterization of the expression of HTm4 (MS4A3), a cell cycle regulator, in human peripheral blood cells and normal and malignant tissues
Authors: Kutok Jeffery L. , Yang Xing , Folkerth Rebecca , Adra Chaker N. 
Published: October 2009

AbstractHTm4 (MS4A3) is a member of a family of four‐transmembrane proteins designated MS4A. MS4A proteins fulfil diverse functions, acting as cell surface signalling molecules and intracellular adapter proteins. Early reports demonstrated that HTm4 is largely restricted to the haematopoietic lineage, and is involved in cell cycle control, via a regulatory interaction with the kinase‐associated phosphatase, cyclin A and cyclin‐dependent kinase 2 (CDK2). Here we describe the expression pattern of HTm4 in peripheral blood cells using gene expression microarray technology, and in normal foetal and adult human tissues, as well as adult human cancers, using tissue microarray technology. Using oligonucleotide microarrays to evaluate HTm4 mRNA, all peripheral blood cell types demonstrated very low levels of HTm4 expression; however, HTm4 expression was greatest in basophils compared to eosinophils, which showed lower levels of HTm4 expression. Very weak HTm4 expression is found in monocytes, granulocytes and B cells, but not in T cells, by lineage specific haematopoietic cell flow cytometry analysis. Interestingly, phytohaemagglutinin stimulation increases HTm4 protein expression in peripheral blood CD4‐T‐lymphocytes over nearly undetectable baseline levels. Western blotting and immunohistochemical studies show strong HTm4 expression in the developing haematopoietic cells of human foetal liver. Immunohistochemical studies on normal tissue microarrays confirmed HTm4 expression in a subset of leucocytes in nodal, splenic tissues and thymic tissue, and weak staining in small numbers of cell types in non‐haematopoietic tissues. Human foetal brain specimens from 19 to 31 gestational weeks showed that the strongest‐staining cells are ventricular zone cells and the earliest‐born, earliest‐differentiating ‘pioneer’ neurons in the cortical plate, Cajal‐Retzius and, to a lesser extent, subplate‐like neurons. Malignant tissue microarray analysis showed HTm4 expression in a wide variety of adenocarcinomas, including breast, prostate and ovarian. These findings warrant the further study of the role of HTm4 in the cell cycle of both haematopoietic and tumour cells.
Epigenetic modulation of the protein kinase A RIIα (PRKAR2A) gene by histone deacetylases 1 and 2 in human smooth muscle cells
Authors: Karolczak‐Bayatti Magdalena , Loughney Andrew D. , Robson Stephen C. , Europe‐Finner G. Nicholas 
Published: October 2009

AbstractRecently we reported that the expression of the protein kinase A (PKA) regulatory subunit RIIα is dynamically regulated in human smooth muscle cells of the uterus. We showed that expression levels of mRNA/protein were substantially increased during pregnancy and decreased upon labour, changes that were mirrored by particulate type II PKA activity. This implied an important role for RIIα in maintaining uterine quiescence during pregnancy. Consequently the purpose of the present study was to identify potential mechanisms by which expression of the RIIα gene was regulated in this tissue. We indicate here that the three SpI‐III (GC) binding domains within the proximal promoter region of the human RIIα gene may play important roles in modulating expression of the gene in human myometrial cells. We show that all three GC binding domains are involved in binding Sp1, Sp3, histone deacetylase (HDACs) 1/2 and RbAp48 transcriptional complexes. The functional significance of these binding domains was further analysed employing in vitro luciferase reporter assays with full‐length/truncated RIIα promoter constructs. Importantly we show that treatment of primary human myometrial cell cultures with the general class I/II HDAC inhibitor trichostatin A results in an increase in mRNA/protein levels. Moreover the increase in mRNA levels appeared to be preceded by an increase in aH3, PolIIa, Sp3 and HDAC 2 binding to the three SpI‐III (GC) binding sites within the RIIα promoter. These results enable us to provide a model whereby RIIα expression is epigenetically regulated in human myometrial smooth muscle cells by histone deacetylase(s) activity within the GC‐rich proximal promoter region of the gene.
Transport characteristics of a novel peptide platform for CNS therapeutics
Authors: Bertrand Yanick , Currie Jean‐Christophe , Demeule Michel , Régina Anthony , Ché Christian , Abulrob Abedelnasser , Fatehi Dorothy , Sartelet Hervé , Gabathuler Reinhard , Castaigne Jean‐Paul , Stanimirovic Danica , Béliveau Richard 
Published: October 2009

AbstractNew and effective therapeutics that cross the blood‐brain barrier (BBB) are critically needed for treatment of many brain diseases. We characterize here a novel drug development platform that is broadly applicable for the development of new therapeutics with increased brain penetration. The platform is based on the Angiopep‐2 peptide, a sequence derived from ligands that bind to low‐density lipoprotein receptor‐related protein‐1 (LRP‐1), a receptor expressed on the BBB. Fluorescent imaging studies of a Cy5.5Angiopep‐2 conjugate and immunohistochemical studies of injected Angiopep‐2 in mice demonstrated efficient transport across the BBB into brain parenchyma and subsequent co‐localization with the neuronal nuclei‐selective marker NeuN and the glial marker glial fibrillary acidic protein (GFAP). Uptake of [125I]‐Angiopep‐2 into brain endothelial cells occurred by a saturable mechanism involving LRP‐1. The primary sequence and charge of Angiopep‐2 were crucial for its passage across the BBB. Overall, the results demonstrate the significant potential of this platform for the development of novel neurotherapeutics.
A link between the accumulation of DNA damage and loss of multi‐potency of human mesenchymal stromal cells
Authors: Alves Hugo , Munoz‐Najar Ursula , De Wit Jan , Renard Auke J. S. , Hoeijmakers Jan H. J. , Sedivy John M. , Van Blitterswijk Clemens , De Boer Jan 
Published: October 2009

AbstractHuman mesenchymal stromal cells (hMSCs) represent an attractive cell source for clinic applications. Besides being multi‐potent, recent clinical trials suggest that they secrete both trophic and immunomodulatory factors, allowing allogenic MSCs to be used in a wider variety of clinical situations. The yield of prospective isolation is however very low, making expansion a required step toward clinical applications. Unfortunately, this leads to a significant decrease in their stemness. To identify the mechanism behind loss of multi‐potency, hMSCs were expanded until replicative senescence and the concomitant molecular changes were characterized at regular intervals. We observed that, with time of culture, loss of multi‐potency was associated with both the accumulation of DNA damage and the respective activation of the DNA damage response pathway, suggesting a correlation between both phenomena. Indeed, exposing hMSCs to DNA damage agents led to a significant decrease in the differentiation potential. We also showed that hMSCs are susceptible to accumulate DNA damage upon in vitro expansion, and that although hMSCs maintained an effective nucleotide excision repair activity, there was a progressive accumulation of DNA damage. We propose a model in which DNA damage accumulation contributes to the loss of differentiation potential of hMSCs, which might not only compromise their potential for clinical applications but also contribute to the characteristics of tissue ageing.
BiP, an endoplasmic reticulum chaperone, modulates the development of morphine antinociceptive tolerance
Authors: Dobashi Tamae , Tanabe Serabi , Jin Hisayo , Mimura Naoya , Yamamoto Tatsuo , Nishino Takashi , Aoe Tomohiko 
Published: October 2009

AbstractMorphine is a potent analgesic, but the molecular mechanism for tolerance formation after repeated use is not fully understood. Binding immunoglobulin protein (BiP) is an endoplasmic reticulum (ER) chaperone that is central to ER function. We examined knock‐in mice expressing a mutant BiP with the retrieval sequence deleted in order to elucidate physiological processes that are sensitive to BiP functions. We tested the thermal antinociceptive effect of morphine in heterozygous mutant BiP mice in a hot plate test. Paw withdrawal latencies before and after a single administration of morphine were not significantly different between the wild‐type and mutant BiP mice. Repeated morphine administration caused the development of morphine tolerance in the wild‐type mice. The activation of glycogen synthase kinase 3b (GSK‐3b) was associated with morphine tolerance, because an inhibitor of GSK‐3β prevented it. On the other hand, the mutant BiP mice showed less morphine tolerance, and the activation of GSK‐3b was suppressed in their brain. These results suggest that BiP may play an important role in the development of morphine tolerance. Furthermore, we found that a chemical chaperone which improves ER protein folding capacity also attenuated the development of morphine tolerance in wild‐type mice, suggesting a possible clinical application of chemical chaperones in preventing morphine tolerance.
Effect of irreversibly glycated LDL in human vascular smooth muscle cells: lipid loading, oxidative and inflammatory stress
Authors: Sima Anca V. , Botez Gabriela M. , Stancu Camelia S. , Manea Adrian , Raicu Monica , Simionescu Maya 
Published: October 2009

AbstractThe major complication of diabetes is accelerated atherosclerosis, the progression of which entails complex interactions between the modified low‐density lipoproteins (LDL) and the cells of the arterial wall. Advanced glycation end product‐modified‐LDL (AGE‐LDL) that occurs at high rate in diabetes contributes to diabetic atherosclerosis, but the underlying mechanisms are not fully understood. The aim of this study was to assess the direct effect of AGE‐LDL on human vascular smooth muscle cells (hSMC) dysfunction. Cultured hSMC incubated (24 hrs) with human AGE‐LDL, native LDL (nLDL) or oxidized LDL (oxLDL) were subjected to: (i) quantification of the expression of the receptors for modified LDL and AGE proteins (LRP1, CD36, RAGE) and estimation of lipid loading, (ii) determination of NADPH oxidase activity and reactive oxygen species (ROS) production and (iii) evaluation of the expression of monocyte chemoattractant protein‐1 (MCP‐1). The results show that exposure of hSMC to AGE‐LDL (compared to nLDL) induced: (a) increased NADPH oxidase activity (30%) and ROS production (28%) by up‐regulation of NOX1, NOX4, p22phox and p67phox expression, (b) accumulation of intracellular cholesteryl esters, (c) enhanced gene expression of LRP1 (160%) and CD36 (35%), and protein expression of LRP1, CD36 and RAGE, (d) increased MCP‐1 gene expression (160%) and protein secretion (300%) and (e) augmented cell proliferation (30%). In conclusion, AGE‐LDL activates hSMC (increasing CD36, LRP1, RAGE), inducing a pro‐oxidant state (activation of NADPHox), lipid accumulation and a pro‐inflammatory state (expression of MCP‐1). These results may partly explain the contribution of AGE‐LDL and hSMC to the accelerated atherosclerosis in diabetes.
Fish consumption and school grades in Swedish adolescents: a study of the large general population
Authors: Kim J‐L , Winkvist A , Åberg M AI , Åberg N , Sundberg R , Torén K , Brisman J 
Published: October 2009

AbstractAim:  To study the associations between fish intake and academic achievement as cognitive parameter among Swedish adolescents.Methods:  In 2000, a questionnaire including respiratory items, socioeconomic conditions and dietary information was mailed to all schoolchildren (n = 18 158), aged 15 and living in Västra Götaland region of Sweden. The questionnaire was returned by 10 837 subjects. One year later, the total school grades for each subject who had completed the questionnaire and who included their full personal identification number were obtained from the national registers. Multiple linear regression models were applied to evaluate the association between fish intake and academic grades among 9448 schoolchildren, while adjusting for potential confounders, e.g. parents’ education.Results:  Grades were higher in subjects with fish consumption once a week compared with subjects with fish consumption of less than once a week (reference group) [increment in estimate 14.5, 95% confidence interval (CI) 11.8–17.1]. Grades were even higher in subjects with fish consumption of more than once a week compared with the reference group (increment in estimate 19.9, 95% CI 16.5–23.3). In the model stratified for parents’ education, there were still higher grades among subjects with frequent fish intake in all educational strata (p < 0.01).Conclusion: Frequent fish intake among schoolchildren may provide benefits in terms of academic achievement.
EDUCATIONAL AND PERSONAL HEALTH*
Authors: Haggard Howard W. 
Published: October 2009

AbstractTo me health, health conservation, and the humanitarianism of lessened human suffering, represent the highest of all values— values toward whose achievement no educational effort can be too great.And this education for health, as I conceive it, would be something different—vastly different—from the sort of education which most of us received. We once called education for health by the name of hygiene, personal hygiene. But the hygiene we knew in school is a failure. I have taught for many years what is generally known as hygiene, and I have gained from the experience a thorough conviction of the inadequacy of the so‐called rules of health. I should prefer my students to think logically and broadly and particularly socially on matters of health in these years of faddism, quackery, and the commercial exploitation of the health appeal. I should prefer this rather than that they be able to recite glibly the hygienic dicta of today, many of which will surely become the absurdities of tomorrow just as those of yesterday have become the absurdities of today. The great trouble with rules is, first, in any progressive field the rules change, and second, rules are no sounder than the authority of those who formulate them. The education for personal health—the hygiene if you will—which I should wish to see taught is, to use a trite expression, hygiene with a social significance.
DeBakey, Lois. “Verbal Eccentricities in Scientific Writing,”
Authors: Peterson Louis J. , Whaley Russell F. 
Published: October 2009

Glass, Robert L., Hayden, Jess. “Dental Caries in Seventh Day Adventist Children,”Bakwin, Harry. “Teen‐age Suicides,” Archives of Environmental HealthDixon, Joseph M. et al. “Complications Associated with the Wearing of Contact Lenses,”Frels, Lois. “Who Is It That Visits the Nurse's Office?”Andes, Ann. “A Summary of ‘Some Health Misconceptions of Selected Adult Groups of Columbus, Ohio‘.”Phillips, H. Temple. “Hostels for the Mentally Disordered—Results of a County‐Wide Enquiry,”
RESEARCH OF INTEREST
Authors: Peterson Louis J. , Whaley Russell F. 
Published: October 2009

Dunn, Dorothy F., “Smoking—Academically Speakingl” The Journal of the American College Health Association, 15: 2 (December, 1966), 162–167.Swinehart, James W., “Changes Over Time in Student Reactions to the Surgeon General's Report on Smoking and Health.” American Journal of Public Health and The Nation's Health, 56: 12 (December, 1966), 2023–2027.Education Research Services, “School Health and Nurse Services,” Education Research Services Circular, Number 6, July 1966, 40 pages. Washington, D.C.: National Education Association, American Association of School Administrators and Research Division.Bell, Robert R., “Parent‐Child Conflict in Sexual Values.” The Journal of Social Issues, 22: 2 (April, 1966), 34–44.Timbury, Morag C., Forsyth, William C., and Stevenson, John S., “Garden Fertilizers as a Source of Salmonellae.” The Lancet, 2: 7470 (December 17, 1966), 955.Silver, Larry B., Barton, William, and Dublin, Christina, “Child Abuse Laws—Are They Enough?” Journal of the American Medical Association, 199: 2 (January 9, 1967), 65–68.Gendel, Evalyn S., “Effective Interaction for School Health.” American Journal of Public Health and The Nation's Health. 56:12 (December, 1966), 2133–2138.Block, Jeanne, el al., Clinician's Conceptions of the the Asthmatogenic Mother.” Archives of General Psychiatry, 15: 6 (December, 1966), 610‐318.
RESEARCH OF INTEREST
Authors: Peterson Louis J. , Whaley RUSSELL F. 
Published: October 2009

Klein, Julius, and Phillips, Derek. “From Hard to Soft Drugs: Temporal and Substantive Changes in Drug Usage Among Gangs in a Working‐Class Community,”Kruger, Cynthia. “Do ‘Bad Girls’ Become Good Nurses?”Press, Edward, and Austin, Caroline. “Screening of Preschool Children for Ambylopia,”Duckworth, R. “Fluoride Dentifrice—A Review of Clinical Trials in the United Kingdom,”Gibbs, William L. “Driver Gap Acceptance at Intersections,”Zimmerman, Robert, and Wilson, Elizabeth. “Familial Susceptibility to Acquisition of Group A, B‐Hemolytic Streptococci,”Hartman, A. Arthur, Nicolay, Robert C. and Hurley, Jesse. “Unique Personal Names as a Social Adjustment Factor,”
SEXUAL ATTITUDES OF MOTHERS OF RETARDED CHILDREN
Authors: Turchin Gary 
Published: October 2009

ABSTRACTForty‐four mothers were interviewed to assess their attitudes toward the sexual behavior, sex education, birth control, marriage, and dating of their TMR adolescents. Religion influenced mothers' attitudes toward masturbation and marriage. Although many mothers expected that their children would develop the ability to date, most of them felt that their children would never be able to marry. Mothers wanted sex education taught in the schools, and they desired the formation of parent groups to aid them in understanding the sexuality of their children.
Research of Interest
Authors: Shibuya Ruth R. 
Published: October 2009

Dobelle WG, Mladejovsky MG and Girvin JP: Artificial vision for the blind: Electrical stimulation of visual cortex offers hope for a functional prosthesis.Pliner PL: Effect of external cues on the thinking behavior of obese and normal subjects.Purdom ME, Hyder K and MD Pybas: Effects of saccharin on rats fed chemically defined diets.Stenchever MA, Kunyaz TJ and Allen MA: Chromosome breakage in users of marihuana.
Reviews of Books and Reports
Authors: Ulrich Charles 
Published: October 2009

Book reviewed in this article:Aaron J, Strasser M: Driving Task Instruction.Samuels H: Mental Health.Wortis J: Mental Retardation and Developmental DisabilitiesGilchrist R, Roberts B: Curriculum Development: A Humanized Systems Approach.Piper T: Classroom Management and Behavioral Objectives.Rhodes CR, Head S: A Study of Child Variance.Siegler M, Osmond H: Models of Madness, Models of Medicine.The Volta Review.Numbers M: My Words Fell on Deaf Ears.
Reviews of Books and Reports
Authors: Ulrich Charles 
Published: October 2009

Book reviewed in this article:Nolen WA: Healing: A Doctor In Search Of A Miracle.Englebardt SL: Kids And Alcohol, The Deadliest Drug.Segall A; Vanderschmidt H; Burglass R; and Frostman T: Systematic Course Design for the Health Fields.Deutsch RM: Nutrition Labeling—How it Can Work For You.
Reviews of Books and Reports
Authors: Ulrich Charles 
Published: October 2009

Book reviewed in this article:Fodor JT, Glass LH, Gmur BC, Moore VD, Neilson EA: The Healthful Living Program.Illingsworth RS: Common Symptoms of Disease in Children.Wilkins EM: Clinical Practice of the Dental Hygienist.Grollman EA: Talking About Divorce—A Dialogue Between Parent and Child.Harvey R: Becoming Man.Thygerson AL: Safety‐Concepts and Instruction.Williams MH: Nutritional Aspects of Human Physical and Athletic Performance.