bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-molecule fluorescence multiplexing by multi-parameter spectroscopic detection of nanostructured FRET labels
Authors: Chu Jiachong , Ejaz Ayesha , Lin Kyle M. , Joseph Madeline R. , Coraor Aria E. , Drummond D. Allan , Squires Allison H. 
Published: May 2024

AbstractMultiplexed, real-time fluorescence detection at the single-molecule level can reveal the stoichiometry, dynamics and interactions of multiple molecular species in mixtures and other complex samples. However, fluorescence-based sensing is typically limited to the detection of just 3–4 colours at a time due to low signal-to-noise ratio, high spectral overlap and the need to maintain the chemical compatibility of dyes. Here we engineered a palette of several dozen composite fluorescent labels, called FRETfluors, for multiplexed spectroscopic measurements at the single-molecule level. FRETfluors are compact nanostructures constructed from three chemical components (DNA, Cy3 and Cy5) with tunable spectroscopic properties due to variations in geometry, fluorophore attachment chemistry and DNA sequence. We demonstrate FRETfluor labelling and detection for low-concentration (<100 fM) mixtures of mRNA, dsDNA and proteins using an anti-Brownian electrokinetic trap. In addition to identifying the unique spectroscopic signature of each FRETfluor, this trap differentiates FRETfluors attached to a target from unbound FRETfluors, enabling wash-free sensing. Although usually considered an undesirable complication of fluorescence, here the inherent sensitivity of fluorophores to the local physicochemical environment provides a new design axis complementary to changing the FRET efficiency. As a result, the number of distinguishable FRETfluor labels can be combinatorically increased while chemical compatibility is maintained, expanding prospects for spectroscopic multiplexing at the single-molecule level using a minimal set of chemical building blocks.
Vinpocetine alleviated alveolar epithelial cells injury in experimental pulmonary fibrosis by targeting PPAR-γ/NLRP3/NF-κB and TGF-β1/Smad2/3 pathways
Authors: Hussein Zeena A. , Abu-Raghif Ahmed R. , Tahseen Nibras J. , Rashed Khalid A. , Shaker Nada S. , Fawzi Hayder Adnan 
Published: May 2024

AbstractThis study aimed to investigate the potential anti-fibrotic activity of vinpocetine in an experimental model of pulmonary fibrosis by bleomycin and in the MRC-5 cell line. Pulmonary fibrosis was induced in BALB/c mice by oropharyngeal aspiration of a single dose of bleomycin (5 mg/kg). The remaining induced animals received a daily dose of pirfenidone (as a standard anti-fibrotic drug) (300 mg/kg/PO) and vinpocetine (20 mg/kg/PO) on day 7 of the induction till the end of the experiment (day 21). The results of the experiment revealed that vinpocetine managed to alleviate the fibrotic endpoints by statistically improving (P ≤ 0.05) the weight index, histopathological score, reduced expression of fibrotic-related proteins in immune-stained lung sections, as well as fibrotic markers measured in serum samples. It also alleviated tissue levels of oxidative stress and inflammatory and pro-fibrotic mediators significantly elevated in bleomycin-only induced animals (P ≤ 0.05). Vinpocetine managed to express a remarkable attenuating effect in pulmonary fibrosis both in vivo and in vitro either directly by interfering with the classical TGF-β1/Smad2/3 signaling pathway or indirectly by upregulating the expression of Nrf2 enhancing the antioxidant system, activating PPAR-γ and downregulating the NLRP3/NF-κB pathway making it a candidate for further clinical investigation in cases of pulmonary fibrosis.
Evaluating distributed-learning on real-world obstetrics data: comparing distributed, centralized and local models
Authors: Coutinho-Almeida João , Cruz-Correia Ricardo João , Rodrigues Pedro Pereira 
Published: May 2024

AbstractThis study focused on comparing distributed learning models with centralized and local models, assessing their efficacy in predicting specific delivery and patient-related outcomes in obstetrics using real-world data. The predictions focus on key moments in the obstetric care process, including discharge and various stages of hospitalization. Our analysis: using 6 different machine learning methods like Decision Trees, Bayesian methods, Stochastic Gradient Descent, K-nearest neighbors, AdaBoost, and Multi-layer Perceptron and 19 different variables with various distributions and types, revealed that distributed models were at least equal, and often superior, to centralized versions and local versions. We also describe thoroughly the preprocessing stage in order to help others implement this method in real-world scenarios. The preprocessing steps included cleaning and harmonizing missing values, handling missing data and encoding categorical variables with multisite logic. Even though the type of machine learning model and the distribution of the outcome variable can impact the result, we reached results of 66% being superior to the centralized and local counterpart and 77% being better than the centralized with AdaBoost. Our experiments also shed light in the preprocessing steps required to implement distributed models in a real-world scenario. Our results advocate for distributed learning as a promising tool for applying machine learning in clinical settings, particularly when privacy and data security are paramount, thus offering a robust solution for privacy-concerned clinical applications.
New Canary Islands Roman mediated settlement hypothesis deduced from coalescence ages of curated maternal indigenous lineages
Authors: Cabrera Vicente M. 
Published: May 2024

AbstractNumerous genetic studies have contributed to reconstructing the human history of the Canary Islands population. The recent use of new ancient DNA targeted enrichment and next-generation sequencing techniques on new Canary Islands samples have greatly improved these molecular results. However, the bulk of the available data is still provided by the classic mitochondrial DNA phylogenetic and phylogeographic studies carried out on the indigenous, historical, and extant human populations of the Canary Islands. In the present study, making use of all the accumulated mitochondrial information, the existence of DNA contamination and archaeological sample misidentification in those samples is evidenced. Following a thorough review of these cases, the new phylogeographic analysis revealed the existence of a heterogeneous indigenous Canarian population, asymmetrically distributed across the various islands, which most likely descended from a unique mainland settlement. These new results and new proposed coalescent ages are compatible with a Roman-mediated arrival driven by the exploitation of the purple dye manufacture in the Canary Islands.
Factors which can influence elastic stable intramedullary nailing removal in healed bone cysts in children
Authors: Liu Jiao , Su Yuxi 
Published: May 2024

AbstractElastic stable intramedullary nailing (ESIN) internal fixation is used clinically to treat pathological fractures of bone cysts in children. However, one of the most important complications was removal difficulty. In this study, we aim to analyse the factors which can influence ESIN removal in healed bone cysts in children. From April 2014 to November 2020, the clinical data of 49 children who underwent elastic stable intramedullary nail removal for pathological fractures of the bone cysts in our hospital were retrospectively analysed. The following data, including age, sex, pathological fracture site, with bone graft, number of ESINs, ESIN indwelling time, and extraosseous length of ESIN were collected, and univariate analysis and logistic regression analysis was performed. The frequency of difficulty in ESIN extraction was 44.90% (22/49). The univariate logistic regression analysis showed that age,ESIN indwelling time,with bone garft and extraosseous length of ESIN may be correlated with the difficulty in removing ESIN (P < 0.05), while sex, pathological fracture site, number of ESIN may not be correlated with the difficulty in removing ESIN (P > 0.05).The multivariate logistic regression analysis showed that the ESIN indwelling time was the independent influencing factor for difficulty in removing ESIN (P < 0.05). The factors influencing the ESIN removal in healed bone cysts in children include over 11.79 years old, the long indwelling time of the ESIN(over 10.5 months),with bone graft and short extraosseous length of ESIN(≤ 0.405 cm). These factors influencing ESIN removal in healed bone cysts in children should be considered.
Evaluating the psychometric properties of the simplified Chinese version of PROMIS-29 version 2.1 in patients with hematologic malignancies
Authors: Zhang Qianqian , Zhao Jinying , Liu Yating , Cui Yan , Wang Wen , Li Junjie , Liu Yanxia , Tian Fei , Wang Zhixin , Zhang Huijuan , Liu Guiying , Wu Yun , Li Qiuhuan , Hu Tingyu , Zhang Wen , Xie Wenjun 
Published: May 2024

AbstractThe Patient-Reported Outcomes Measurement Information System 29-item Profile version 2.1 (PROMIS-29 V2.1) is a widely utilized self-reported instrument for assessing health outcomes from the patients’ perspectives. This study aimed to evaluate the psychometric properties of the PROMIS-29 V2.1 Chinese version among patients with hematological malignancy. Conducted as a cross-sectional, this research was approved by the Medical Ethical Committee of the Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College (registration number QTJC2022002-EC-1). We employed convenience sampling to enroll eligible patients with hematological malignancy from four tertiary hospitals in Tianjin, Shandong, Jiangsu, and Anhui province in China between June and August 2023. Participants were asked to complete a socio-demographic information questionnaire, the PROMIS-29 V2.1, and the Functional Assessment of Cancer Therapy-General (FACT-G). We assessed the reliability, ceiling and floor effects, structural, convergent discriminant and criterion validity of the PROMIS-29 V2.1. A total of 354 patients with a mean age of 46.93 years was included in the final analysis. The reliability of the PROMIS-29 V2.1 was affirmed, with Cronbach’s α for the domains ranging from 0.787 to 0.968. Except sleep disturbance, the other six domains had ceiling effects, which were seen on physical function (26.0%), anxiety (37.0%), depression (40.4%), fatigue (18.4%), social roles (18.9%) and pain interference (43.2%), respectively. Criterion validity was supported by significant correlations between the PROMIS-29 V2.1 and FACT-G scores, as determined by the Spearman correlation test (P < 0.001). Confirmatory factor analysis (CFA) indicated a good model fit, with indices of χ2/df (2.602), IFI (0.960), and RMSEA (0.067). The Average Variance Extracted (AVE) values for the seven dimensions of PROMIS-29 V2.1, ranging from 0.500 to 0.910, demonstrated satisfactory convergent validity. Discriminant validity was confirmed by ideal √AVE values. The Chinese version of the PROMIS-29 V2.1 profile has been validated as an effective instrument for assessing symptoms and functions in patients with hematological malignancy, underscoring its reliability and applicability in this specific patient group.
Structure-based epitope prediction and assessment of cross-reactivity of Myrmecia pilosula venom-specific IgE and recombinant Sol g proteins (Solenopsis geminata)
Authors: Buraphaka Hathairat , Dobutr Theerawat , Wiese Michael D. , Lopata Andreas L. , Daduang Sakda 
Published: May 2024

AbstractThe global distribution of tropical fire ants (Solenopsis geminata) raises concerns about anaphylaxis and serious medical issues in numerous countries. This investigation focused on the cross-reactivity of allergen-specific IgE antibodies between S. geminata and Myrmecia pilosula (Jack Jumper ant) venom proteins due to the potential emergence of cross-reactive allergies in the future. Antibody epitope analysis unveiled one predominant conformational epitope on Sol g 1.1 (PI score of 0.989), followed by Sol g 2.2, Sol g 4.1, and Sol g 3.1. Additionally, Pilosulin 1 showed high allergenic potential (PI score of 0.94), with Pilosulin 5a (PI score of 0.797) leading in B-cell epitopes. The sequence analysis indicated that Sol g 2.2 and Sol g 4.1 pose a high risk of cross-reactivity with Pilosulins 4.1a and 5a. Furthermore, the cross-reactivity of recombinant Sol g proteins with M. pilosula-specific IgE antibodies from 41 patients revealed high cross-reactivity for r-Sol g 3.1 (58.53%) and r-Sol g 4.1 (43.90%), followed by r-Sol g 2.2 (26.82%), and r-Sol g 1.1 (9.75%). Therefore, this study demonstrates cross-reactivity (85.36%) between S. geminata and M. pilosula, highlighting the allergenic risk. Understanding these reactions is vital for the prevention of severe allergic reactions, especially in individuals with pre-existing Jumper Jack ant allergy, informing future management strategies.
Investigating changes in the electric field profiles of transverse modes in fibres due to bending
Authors: Vazeerpour Sajjad , Shayganmanesh Mahdi , Dorranian Davoud 
Published: May 2024

AbstractA model is presented for calculating the electric field profiles of transverse modes in fibres. Using this model, the electric field propagation of transverse modes was calculated and compared in both bent and straight Yb and Ho fibres. Our simulations revealed that the field intensities of the modes did not consistently increase or decrease by bending as expected, but rather fluctuated as the bending radii of the fibres changed from 1 to 100 cm. Additionally, it was observed that at certain bending radii, the modes of the fibres became deformed and transitioned into higher modes. Furthermore, the extent to which the modes stretched towards cladding due to bending was calculated. To provide a more comprehensive analysis of the results and investigate the effects of the physical parameters of the fibres on the bent fibre modes, simulation results were presented for two different V numbers and core radii.
Deceleration of kicked objects due to the Galactic potential
Authors: Disberg P. , Gaspari N. , Levan A. J. 
Published: May 2024

Context.Various stellar objects experience a velocity kick at some point in their evolution. These include neutron stars and black holes at their birth, or binary systems when one of the two components goes supernova. For most of these objects, the magnitude of the kick and its impact on the object dynamics remains a topic of debate.Aims.We investigate how kicks alter the velocity distribution of objects born in the Milky Way disc, both immediately after the kick and at later times, and whether these kicks are encoded in the observed population of Galactic neutron stars.Methods.We simulated the Galactic trajectories of point masses on circular orbits in the disc after being perturbed by an isotropic kick, with a Maxwellian distribution whereσ = 265 km s−1. Then, we simulated the motion of these point masses for 200 Myr. These trajectories were then evaluated, either for the Milky Way population as a whole or for those passing within two kiloparsecs of the Sun, to get the time evolution of the velocities.Results.During the first 20 Myr, the bulk velocity of kicked objects becomes temporarily aligned with the cylindrical radius, implying an anisotropy in the velocity orientations. Beyond this age, the velocity distribution shifts towards lower values and settles to a median of ∼200 km s−1. Around the Sun, the distribution also loses its upper tail, primarily due to unbound objects escaping the Galaxy. We compared this to the velocities of Galactic pulsars and find that pulsars show a similar evolution with characteristic age.Conclusions.The shift in the velocity distribution is due to bound objects spending most of their orbits at larger radii after the kick. They are, therefore, decelerated by the Galactic potential. We find the same deceleration for nearby objects and the total population, and conclude that it is also observed in Galactic pulsars. Because of this effect, the (scalar) speeds of old neutron stars provide little information about their kicks at birth.
Gastrointestinal adverse reaction to food (GARF) and endoscopic confocal laser endomicroscopy (eCLE)
Authors: Frieling Thomas , Gjini Besmir , Melchior Ilka , Euler Philipp , Kreysel Christian , Kalde Sigrid , Krummen Britta , Kiesslich Ralf , Hemmerlein Bernhard 
Published: May 2024

Abstract
Background and study aims Gastrointestinal adverse reaction to food (GARF) is reported frequently in the general population and even more in patients with disorders of the gut brain axis. However, there is a significant difference between self-reported and objective proven GARF. The aim of the study was to characterize a mucosal correlate of GARF by endoscopic confocal laser endomicroscopy (eCLE) with duodenal food challenge (DFC).
Patients and methods In an observational and proof of concept study we evaluated 71 patients with disorders of the gut brain axis without (group I, n=19) and with (group II, n=52) GARF by eCLE and DFC. Spontaneous and food induced transfer of fluorescein into duodenal lumen was detected 10 minutes following intravenously application of fluorescein and 10 minutes after DFC.
Results According to Rom IV, the patients (group I/II) could be classified as irritable bowel syndrome (IBS) 32%/31%, functional abdominal pain without changes in bowel movement 47 %/48 %, functional abdominal bloating/distension 0 %/10 %, functional diarrhea 5 %/ 2 %, and unspecified functional bowel disorder 16 %/10 %, respectively. 21 %/27 % of the patients responded with a fluorescein leakage into the duodenal lumen before and 74 %/69 % following to DFC. Frequency rank order of food components that induced a response were soy (55.5 %/60 %), wheat (60 %/45.5 %), egg (35.7 %/8.3), milk (30 %/18.2 %) and yeast (10 %/6.6 %), respectively. Histology of duodenal biopsies, number, form and distribution of intraepithelial lymphocytes and mucosal mast cells as well as mast cell function were normal. Overall, 14 %/79 % reported main symptom benefit following a food exclusion therapy according to eCLE and DFC that was significant different between the groups.
Conclusion The results of our study indicate that eCLE with DFC is a technique to clinically evaluate patients with disorders of the gut brain axis and GARF resulting in a high proportion of patients reporting symptom benefit upon food exclusion dietary advice focussed on the results of eCLE.
Value of vendor-agnostic deep learning image denoising in brain computed tomography: A multi-scanner study
Authors: Kapper Christian , Müller Lukas , Kronfeld Andrea , Abello Mercado Mario Alberto , Altmann Sebastian , Grauhan Nils , Graafen Dirk , Brockmann Marc A. , Othman Ahmed E. 
Published: May 2024

AbstractTo evaluate the effect of a vendor-agnostic deep learning denoising (DLD) algorithm on diagnostic image quality of non-contrast cranial computed tomography (ncCT) across five CT scanners.This retrospective single-center study included ncCT data of 150 consecutive patients (30 for each of the five scanners) who had undergone routine imaging after minor head trauma. The images were reconstructed using filtered back projection (FBP) and a vendor-agnostic DLD method. Using a 4-point Likert scale, three readers performed a subjective evaluation assessing the following quality criteria: overall diagnostic image quality, image noise, gray matter-white matter differentiation (GM-WM), artifacts, sharpness, and diagnostic confidence. Objective analysis included evaluation of noise, contrast-to-noise ratio (CNR), signal-to-noise ratio (SNR), and an artifact index for the posterior fossa.In subjective image quality assessment, DLD showed constantly superior results compared to FBP in all categories and for all scanners (p<0.05) across all readers. The objective image quality analysis showed significant improvement in noise, SNR, and CNR as well as for the artifact index using DLD for all scanners (p<0.001).The vendor-agnostic deep learning denoising algorithm provided significantly superior results in the subjective as well as in the objective analysis of ncCT images of patients with minor head trauma concerning all parameters compared to the FBP reconstruction. This effect has been observed in all five included scanners.
Healing of Haemorrhoidal Disease with Homoeopathy – A Case Series
Authors: Kumari Punam , Verma Renu , Kumar Shivam 
Published: May 2024

AbstractHaemorrhoidal disease is recognised as one of the most common proctologic medical conditions in general population. This is a case series of five patients suffering from haemorrhoidal disease having intense pain while defecation, swelling around anus, bleeding per rectum, itching in anus and prolapsed internal haemorrhoids which were healed completely by the individualised homoeopathy without any surgical intervention. Haemorrhoidal disease symptom score and a disease-specific short health score were used to assess the symptom severity and improvement in quality of life of the patients, measured at baseline, third and at sixth month of the treatment. The possible causal attribution of the changes was assessed. The cases were reported according to the HOM-CASE guidelines. Homoeopathic medicines such as Acidum nitricum, Aesculus hippocastanum, Lycopodium clavatum, Nux vomica and Thuja occidentalis were found useful.
Prudential net zero transition plans: the potential of a new regulatory instrument
Authors: Dikau Simon , Robins Nick , Smoleńska Agnieszka , van’t Klooster Jens , Volz Ulrich 
Published: May 2024

AbstractNet zero transition plans are a promising additional instrument for prudential supervisors to assess, address and bring distant financial risks into the present. To date, transition plans have primarily emerged as non-financial disclosure requirement and as such, their prudential application has been limited. In this article, we discuss the role that transition plans can play as a new regulatory tool in banking supervision. The article outlines steps towards incorporating transition plans into prudential policy, thereby enabling supervisors to effectively use transition plans as a forward-looking instrument to better manage and overcome some of the challenges associated with climate transition risks.
Drivers of Inclusive Development: An Empirical Investigation
Authors: Morosoli Christoph , Draper Peter , Freytag Andreas , Schuhmann Sebastian 
Published: May 2024

AbstractConcerns about socially uneven progress and inequality have regained public attention (including that of many populist politicians). The purpose of this paper is to identify the economic policies as well as economic factors that facilitate inclusive development. This paper is a first attempt to empirically estimate the drivers of inclusive development. For our empirical assessments, we apply the Multidimensional Inclusiveness Index suggested by Dörffel and Schuhmann (2022) in a panel OLS regression setup with fixed effects (FE) and GMM estimations for up to 178 countries and a time frame ranging from 1980 to 2018. In FE regressions, we find robust associations with inflation as well as financial sector development in the short and long-run, trade/GDP in the long-run. The GMM results point only to inflation and trade as significant drivers in the long-run and investment in the short run. These results suggest that accessible and well-functioning financial markets paired with low rates of inflation and high trade openness take on a more critical role than government spending. Our results suggest that rudiments of the Washington consensus could still guide the promotion of inclusive development.