bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of genome damage and its relation to oxidative stress induced by glyphosate in human lymphocytes in vitro
Authors: Mladinic Marin , Berend Suzana , Vrdoljak Ana Lucic , Kopjar Nevenka , Radic Bozica , Zeljezic Davor 
Published: April 2009

AbstractIn the present study we evaluated the genotoxic and oxidative potential of glyphosate on human lymphocytes at concentrations likely to be encountered in residential and occupational exposure. Testing was done with and without metabolic activation (S9). Ferric‐reducing ability of plasma (FRAP), thiobarbituric acid reactive substances (TBARS) and the hOGG1 modified comet assay were used to measure glyphosate's oxidative potential and its impact on DNA. Genotoxicity was evaluated by alkaline comet and analysis of micronuclei and other nuclear instabilities applying centromere probes. The alkaline comet assay showed significantly increased tail length (20.39 μm) and intensity (2.19%) for 580 μg/ml, and increased tail intensity (1.88%) at 92.8 μg/ml, compared to control values of 18.15 μm for tail length and 1.14% for tail intensity. With S9, tail length was significantly increased for all concentrations tested: 3.5, 92.8, and 580 μg/ml. Using the hOGG1 comet assay, a significant increase in tail intensity was observed at 2.91 μg/ml with S9 and 580 μg/ml without S9. Without S9, the frequency of micronuclei, nuclear buds and nucleoplasmic bridges slightly increased at concentrations 3.5 μg/ml and higher. The presence of S9 significantly elevated the frequency of nuclear instabilities only for 580 μg/ml. FRAP values slightly increased only at 580 μg/ml regardless of metabolic activation, while TBARS values increased significantly. Since for any of the assays applied, no clear dose‐dependent effect was observed, it indicates that glyphosate in concentrations relevant to human exposure do not pose significant health risk. Environ. Mol. Mutagen. 2009. © 2009 Wiley‐Liss, Inc.
Sediment storage and transport in Pancho Rico Valley during and after the Pleistocene‐Holocene transition, Coast Ranges of central California (Monterey County)
Authors: García Antonio F. , Mahan Shannon A. 
Published: April 2009

AbstractFactors influencing sediment transport and storage within the 156·6 km2 drainage basin of Pancho Rico Creek (PRC), and sediment transport from the PRC drainage basin to its c. 11 000 km2 mainstem drainage (Salinas River) are investigated. Numeric age estimates are determined by optically stimulated luminescence (OSL) dating on quartz grains from three sediment samples collected from a ‘quaternary terrace a (Qta)’ PRC terrace/PRC‐tributary fan sequence, which consists dominantly of debris flow deposits overlying fluvial sediments. OSL dating results, morphometric analyses of topography, and field results indicate that the stormy climate of the Pleistocene‐Holocene transition caused intense debris‐flow erosion of PRC‐tributary valleys. However, during that time, the PRC channel was backfilled by Qta sediment, which indicates that there was insufficient discharge in PRC to transport the sediment load produced by tributary‐valley denudation. Locally, Salinas Valley alluvial stratigraphy lacks any record of hillslope erosion occurring during the Pleistocene‐Holocene transition, in that the alluvial fan formed where PRC enters the Salinas Valley lacks lobes correlative to Qta. This indicates that sediment stripped from PRC tributaries was mostly trapped in Pancho Rico Valley despite the relatively moist climate of the Pleistocene‐Holocene transition.Incision into Qta did not occur until PRC enlarged its drainage basin by c. 50% through capture of the upper part of San Lorenzo Creek, which occurred some time after the Pleistocene‐Holocene transition. During the relatively dry Holocene, PRC incision through Qta and into bedrock, as well as delivery of sediment to the San Ardo Fan, were facilitated by the discharge increase associated with stream‐capture. The influence of multiple mechanisms on sediment storage and transport in the Pancho Rico Valley‐Salinas Valley system exemplifies the complexity that (in some instances) must be recognized in order to correctly interpret terrestrial sedimentary sequences in tectonically active areas. Copyright © 2009 John Wiley & Sons, Ltd.
Reducing the peak and average power for OFDM systems using QAM by constellation shaping
Authors: Sterian Corneliu Eugen D. , Wesolowski Krzysztof , Wu Yi , Pätzold Matthias 
Published: April 2009

AbstractIn this paper, we present a shell mapping scheme to reduce the peak and average power of orthogonal frequency division multiplexing (OFDM) signals using quadrature amplitude modulation (QAM) by constellation shaping. Our approach can be considered as an extension of the shell mapping technique introduced originally in the V.34 recommendation of ITU‐T. The shell mapping method uses multiple cost functions, each having the OFDM block lengthNas a parameter. The cost functions are presented in closed form for anyN. The main advantage of the proposed scheme is that the peak and average power can be reduced without sacrificing the data rate, and no side information is needed at the receive side. We apply our constellation shaping technique to OFDM systems withNequal to 16, 32 and 128 subcarriers. The results show that the peak and average power decrease with increasing values ofN. This advantage is partly offset by a slightly higher system complexity. Copyright © 2009 John Wiley & Sons, Ltd.
Interaction of a phosphorus‐based FR, a nanoclay and PA6—Part 1: Interaction of FR and nanoclay
Authors: Ramani Alwar , Hagen Martin , Hereid Johan , Zhang Jianping , Bakirtzis Dimitri , Delichatsios Michael 
Published: April 2009

AbstractThe thermal decomposition of organophosphorus fire‐retardant (OP1311) and/ or organonanoclay (Cloisite 30B) is hereby investigated employing thermogravimetric analysis (TGA), to give an insight into their intrinsic behaviour and interaction in polymer nanocomposites for fire safety applications, because the addition of OP1311 and Cloisite 30B in Polyamide 6 (PA6) seems to have a synergistic effect on the thermal decomposition of PA6 (part 2 of the paper). An important objective of this research was to determine to what extent phosphorus components escape in the gaseous phase, which will affect the heat of combustion of the fire‐retarded polymer. The decomposition products arising from pyrolysis and combustion are investigated by means of Fourier transform infrared spectroscopy. Under pyrolytic conditions, the inclusion of Cloisite 30B into OP1311 (FR) shows a synergistic effect on the initial mass loss at low temperature of ∼280–420°C and leads to the acceleration of the thermal degradation process. While the DTG curve of Cloisite 30B shows two distinct degradation peaks (steps) that of OP1311 and OP1311 plus Cloisite 30B show four degradation steps. TGA measurements of OP1311 in nitrogen show more mass loss than in air, whereas Cloisite 30B gives similar amounts of mass loss in air and nitrogen. In nitrogen, the major evolved gaseous species from Cloisite 30B alone are hydrocarbons, 2‐(diethylamino)ethanol and water, whereas the evolved gases from that of OP1311 at ∼320°C are mainly water, at ∼420°C, carbon dioxide, water and ammonia and at 480–570°C diethylphosphinic acid. Under thermo‐oxidative conditions, the gases evolved are mainly carbon dioxide and water from both Cloisite 30B and OP1311. Copyright © 2009 John Wiley & Sons, Ltd.
On the stability and dissipation of wall boundary conditions for compressible flows
Authors: Lamarque N. , Porta M. , Nicoud F. , Poinsot T. 
Published: April 2009

AbstractCharacteristic formulations for boundary conditions have demonstrated their effectiveness to handle inlets and outlets, especially to avoid acoustic wave reflections. At walls, however, most authors use simple Dirichlet or Neumann boundary conditions, where the normal velocity (or pressure gradient) is set to zero. This paper demonstrates that there are significant differences between characteristic and Dirichlet methods at a wall and that simulations are more stable when using walls modelled with a characteristic wave decomposition. The derivation of characteristic methods yields an additional boundary term in the continuity equation, which explains their increased stability. This term also allows to handle the two acoustic waves going towards and away from the wall in a consistent manner. Those observations are confirmed by stability matrix analysis and one‐ and two‐dimensional simulations of acoustic modes in cavities. Copyright © 2009 John Wiley & Sons, Ltd.
Gefitinib ‐ a potential new treatment option in lung cancer
Authors: Blackhall Fiona , Chaplin Steve 
Published: April 2009

AbstractLung cancer is the second most common cancer in the UK, accounting for one in seven new cases, 24% of cancer deaths among men and 20% among women.1 In this article, Dr Fiona Blackhall and Steve Chaplin consider the use of gefitinib for lung cancer its mechanism of action, efficacy and adverse effects are described, followed by its effect on quality of life and its potential place in disease management. Pharmacist Geoff Saunders provides a commentary on the article and remarks on the selective use of gefitinib in lung cancer therapy. Copyright © 2009 John Wiley & Sons, Ltd.
Agomelatine: a new treatment for depression
Authors: Deakin Bill 
Published: April 2009

AbstractIt is estimated that depression carries the greatest burden of disability in established economies. A range of treatments are available, and selective serotonin reuptake inhibitors (SSRIs) are currently the most commonly prescribed class of antidepressant. In this article, the author discusses agomelatine, a novel antidepressant recently licensed by the European Medicines Agency (EMEA), the evidence for its use, and its potential place in therapy. Copyright © 2009 John Wiley & Sons, Ltd.
Calcium aluminate coated and uncoated free form fabricated CoCr implants: A comparative study in rabbit
Authors: Palmquist A. , Jarmar T. , Hermansson L. , Emanuelsson L. , Taylor A. , Taylor M. , Engqvist H. , Thomsen P. 
Published: April 2009

AbstractThe purpose of this study was to compare the integration in bone of uncoated free form fabricated cobalt chromium (CoCr) implants to the same implant with a calcium aluminate coating. The implants of cylindrical design with a pyramidal surface structure were press‐fit into the limbs of New Zealand white rabbits. After 6 weeks, the rabbits were sacrificed, and samples were retrieved and embedded. Ground sections were subjected to histological analysis and histomorphometry. The section counter part was used for preparing an electron transparent transmission electron microscopy sample by focused ion beam milling. Calcium aluminate dip coating provided a significantly greater degree of bone contact than that of the native CoCr. The gibbsite hydrate formed in the hardening reaction of the calcium aluminate was found to be the exclusive crystalline phase material in direct contact with bone. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
A study on the influence of biocompatible composites with bioactive ligands toward their effect on cell adhesion and growth for the application in bone tissue engineering
Authors: Roeker Stefanie , Böhm Stefanie , Diederichs Solvig , Bode Franziska , Quade Antje , Korzhikov Victor , van Griensven Martijn , Tennikova Tatiana B. , Kasper Cornelia 
Published: April 2009

AbstractThe aim of this study was the transformation of the macroporous zirconium dioxide ceramic Sponceram® into a biomimetic composite material. To enhance the adhesion of cells and to induce their differentiation into osteoblasts poly‐L‐lysine and BMP‐2 were coupled to polymers and copolymers based on 2‐deoxy‐N‐methacrylamido‐D‐glucose (ox.p(MAG) and p(MVA)) used as spacer, which were adsorbed onto the ceramic surface. The development of the composite materials was validated step by step qualitatively and quantitatively. The bioactive potential of the composite materials was tested under static and dynamic conditions using an osteoblastic model cell line and human mesenchymal stem cells. Both composite materials showed potential to enhance the adhesion of cells in the first 10 days of their cultivation. One of the composite materials, namely Sponceram®/ox.p(MAG)‐BMP‐2, was tested into a rotating‐bed bioreactor with regard to its osteogenic differentiation‐inducing potential. Compared with Sponceram® modified with BMP‐2 without a polymer spacer, it showed increased expression of osteogenic markers determined by PCR analysis. In summary, the in vitro testing of the developed composite materials demonstrated a promising potential for their application as biomimetic scaffold materials with controllable properties. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
Comparative analysis of the performance of commonly available density functionals in the determination of geometrical parameters for zinc complexes
Authors: Sousa Sérgio F. , Carvalho Emanuela S. , Ferreira Diana M. , Tavares Isabel S. , Fernandes Pedro A. , Ramos Maria João , Gomes JosÉ A. N. F. 
Published: April 2009

AbstractA set of 44 Zinc‐ligand bond‐lengths and of 60 ligand‐metal‐ligand bond angles from 10 diverse transition‐metal complexes, representative of the coordination spheres of typical biological Zn systems, were used to evaluate the performance of a total of 18 commonly available density functionals in geometry determination. Five different basis sets were considered for each density functional, namely two all‐electron basis sets (a double‐zeta and triple‐zeta formulation) and three basis sets including popular types of effective‐core potentials: Los Alamos, Steven‐Basch‐Krauss, and Stuttgart‐Dresden. The results show that there are presently several better alternatives to the popular B3LYP density functional for the determination of Zn‐ligand bond‐lengths and angles. BB1K, MPWB1K, MPW1K, B97‐2 and TPSS are suggested as the strongest alternatives for this effect presently available in most computational chemistry software packages. In addition, the results show that the use of effective‐core potentials (in particular Stuttgart‐Dresden) has a very limited impact, in terms of accuracy, in the determination of metal‐ligand bond‐lengths and angles in Zinc‐complexes, and is a good and safe alternative to the use of an all‐electron basis set such as 6‐31G(d) or 6‐311G(d,p). © 2009 Wiley Periodicals, Inc. J Comput Chem 2009
Hydrogenation of m‐dinitrobenzene to m‐phenylenediamine over La2O3‐promoted Ni/SiO2 catalysts
Authors: Liu Yingxin , Wei Zuojun , Deng Shuguang , Zhang Jiyan 
Published: April 2009

AbstractBACKGROUND: Liquid‐phase catalytic hydrogenation of m‐dinitrobenzene is an environmentally friendly routine for m‐phenylenediamine production. The key to increasing product yield is to develop catalysts with high catalytic performance. In this work, La2O3‐modified Ni/SiO2 catalysts were prepared and applied to the hydrogenation of m‐dinitrobenzene to m‐phenylenediamine. The effect of La2O3 loading on the properties of Ni/SiO2 was investigated. The reaction kinetic study was performed in ethanol over Ni/3%La2O3–SiO2 catalyst, in order to clarify the reaction mechanism of m‐dinitrobenzene hydrogenation.RESULTS: It was found that the activity of the silica supported nickel catalysts is obviously influenced by La2O3 loading. Ni/3%La2O3–SiO2 catalyst exhibits high activity owing to its well dispersed nickel species, with conversion of m‐dinitrobenzene and yield of m‐phenylenediamine up to 97.1% and 94%, respectively. The results also show that Ni/3%La2O3–SiO2 catalyst can be reused at least six times without significant loss of activity.CONCLUSION: La2O3 shows strong promotion of the effect of Ni/SiO2 catalyst for liquid‐phase hydrogenation of m‐dinitrobenzene. La2O3 loading can affect the properties of Ni/SiO2 catalyst. Based on the study of m‐dinitrobenzene hydrogenation kinetics over Ni/3%La2O3–SiO2 catalyst, a possible reaction mechanism is proposed. Copyright © 2009 Society of Chemical Industry
Retracted:Effluent treatment using a bipolar electrochemical reactor with rotating cylinder electrodes of woven‐wire meshes
Authors: Grau Javier M. , Bisang José M. 
Published: April 2009

AbstractThe following article from the Journal of Chemical Technology and Biotechnology, ‘Effluent treatment using a bipolar electrochemical reactor with rotating cylinder electrodes of woven‐wire meshes’ by Javier M. Grau and José M. Bisang (DOI: 10.1002/JCTB.2202), published online on 28 April 2009 in Wiley InterScience (www.interscience.wiley.com), has been retracted by agreement between the authors and John Wiley & Sons, Ltd. The retraction has been agreed because a duplicate version of the article was published on 6 May 2009 (DOI: 10.1002/JCTB.2199). This occurred due to a technical error. The publishers wish to apologise for this error.
Conflict and resolution between phylogenies inferred from molecular and phenotypic data sets for hagfish, lampreys, and gnathostomes
Authors: Near Thomas J. 
Published: April 2009

AbstractOne of the most problematic issues in vertebrate phylogenetics is the disagreement between phenotypic and molecular inferences regarding the relationships among hagfishes, lampreys, and gnathostomes. Phenotypic characters support monophyly of lampreys and gnathostomes, whereas nearly all published analyses of molecular data sets support monophyly of hagfishes and lampreys. In this study I present results of phylogenetic analyses of combined phenotypic and molecular data sets that focus on relationships among hagfishes, lampreys, and gnathostomes. Maximum parsimony analyses of 115 phenotypic characters combined with 4,638 rRNA sites and more than 10,000 amino acids each result in monophyly of lampreys and gnathostomes, demonstrating that the addition of relatively few phenotypic characters can alter phylogenetic inferences from large molecular data sets. On the other hand, Bayesian analyses of the combined data sets support monophyly of hagfish and lampreys, indicating that model‐based analyses may be prone to data “swamping,” where the phylogenetic signal of the larger molecular data sets overwhelm the signal present in the much smaller phenotypic data set. Nodes that relate hagfish and lampreys were recovered at a low frequency in parametric bootstrapping analyses, indicating that the timing of diversification among hagfishes, lampreys, and gnathostomes has created a difficult phylogenetic problem for molecular data. The fact that addition of relatively few phenotypic characters can alter phylogenetic inferences of cyclostome monophyly obtained from molecular data sets, and the inability of simulated data sets to recover key nodes in the craniate phylogeny provide reasons to view the strong support for cyclostome monophyly inferred from molecular data sets with a measured degree of skepticism. J. Exp. Zool. (Mol. Dev. Evol.) 312B:749–761, 2009. © 2009 Wiley‐Liss, Inc.
Fin regeneration from tail segment with musculature, endoskeleton, and scales
Authors: Shao Jinhui , Qian Xiaojing , Zhang Chengxia , Xu Zenglu 
Published: April 2009

AbstractIt is well known that fish caudal fins can be completely regenerated after fin amputation. Although much research on fin regeneration has been carried out, there have been very few reports regarding fin regeneration after tail amputation. In this study, we used grass carp, common carp, koi carp, and zebrafish as experimental organisms. Some caudal fins could be distinctly regenerated in 2 weeks after tail amputation. After all‐trans‐retinoic acid treatment and tail amputation, zebrafish were unable to regenerate caudal fins that could be seen with the naked eye. However, after tail amputation, more than half of the zebrafish tested were able to regenerate caudal fins. Caudal fin regeneration depended on the presence of musculature and endoskeleton at the site of amputation. These caudal fins arose from segments of the endoskeleton, which contrast with currently accepted knowledge. J. Exp. Zool. (Mol. Dev. Evol.) 312B:762–769, 2009. © 2009 Wiley‐Liss, Inc.
Atomic force microscopy study of the role of LPS O‐antigen on adhesion ofE. coli
Authors: Strauss Joshua , Burnham Nancy A. , Camesano Terri A. 
Published: April 2009

AbstractThe O‐antigen is a highly variable component of the lipopolysaccharide (LPS) amongEscherichia colistrains and is useful for strain identification and assessing virulence. While the O‐antigen has been chemically well characterized in terms of sugar composition, physical properties such as O‐antigen length ofE. coliLPS have not been well studied, even though LPS length is important for determining binding of bacteria to biomolecules and epithelial cells. Atomic force microscopy (AFM) was used to characterize the physicochemical properties of the LPS of eightE. colistrains. Steric repulsion between the AFM tip (silicon nitride) and theE. colicells was measured and modeled, to determine LPS lengths for three O157 and two O113E. colistrains, and three control (K12) strains that do not express the O‐antigen. For strains with an O‐antigen, the LPS lengths ranged from 17 ± 10 to 37 ± 9 nm, and LPS length was positively correlated with the force of adhesion (Fadh). Longer lengths of LPS may have allowed for more hydrogen bonding between the O‐antigen and silanol groups of the AFM silicon nitride tip, which controlled the magnitude ofFadh. For control strains, LPS lengths ranged from 3 ± 2 to 5 ± 3 nm, and there was no relationship between LPS length and adhesion force between the bacterium and the silicon nitride tip. In the absence of the O‐antigen, we attributedFadhto electrostatic interactions with lipids in the bacterial membrane. Copyright © 2009 John Wiley & Sons, Ltd.
Involvement of histidines 11, 15 and 19 in the binding of zinc to the fusogenic H5WYG peptide
Authors: Buré Corinne , Pichon Chantal , Midoux Patrick 
Published: April 2009

AbstractThe histidine‐rich GLFHAIAHFIHGGWHGLIHGWYG peptide (H5WYG) coordinates a Zn2+ ion and forms a stable peptide–metal complex promoting membrane fusion at physiologic pH. In our previous article titled ‘Histidine‐rich peptide: evidence for a single zinc‐binding site on H5WYG peptide that promotes membrane fusion at neutral pH’ in Journal of Mass Spectrometry (2009, 44, 81–89), tandem mass spectrometry experiments have provided evidence for the binding of a single Zn2+ ion to H5WYG and suggested that this binding is shared between H11, H19 and probably H15 residues. To clarify the involvement of these histidine residues in the binding to the Zn2+ ion and especially to remove the doubt about the implication of the H15 residue, here we have used three H5WYG mutants termed H5WYGH11A, H5WYGH15A and H5WYGH19A, whose H11, H15 and H19 residues were replaced with an alanine residue. The novelty introduced by these new tandem mass spectrometry experiments performed with the mutants is the demonstration that H15 is involved in the binding of the single Zn2+ ion and that it may even favour the setting of another Zn2+ ion. Thus, the three histidines H11, H15 and H19 could lead to a specific structuring of H5WYG that can promote membrane fusion upon the binding of zinc. Copyright © 2009 John Wiley & Sons, Ltd.