bluuebunny/crossref_metadata_2025_split huggingface dataset:
CERN-based experiments and Monte-Carlo studies on focused dose delivery with very high energy electron (VHEE) beams for radiotherapy applications
Authors: Whitmore L. , Mackay R. I. , van Herk M. , Korysko P. , Farabolini W. , Malyzhenkov A. , Corsini R. , Jones R. M. 
Published: May 2024

AbstractVery High Energy Electron (VHEE) beams are a promising alternative to conventional radiotherapy due to their highly penetrating nature and their applicability as a modality for FLASH (ultra-high dose-rate) radiotherapy. The dose distributions due to VHEE need to be optimised; one option is through the use of quadrupole magnets to focus the beam, reducing the dose to healthy tissue and allowing for targeted dose delivery at conventional or FLASH dose-rates. This paper presents an in depth exploration of the focusing achievable at the current CLEAR (CERN Linear Electron Accelerator for Research) facility, for beam energies >200 MeV. A shorter, more optimal quadrupole setup was also investigated using the TOPAS code in Monte Carlo simulations, with dimensions and beam parameters more appropriate to a clinical situation. This work provides insight into how a focused VHEE radiotherapy beam delivery system might be achieved.
Identification of Gα12-vs-Gα13-coupling determinants and development of a Gα12/13-coupled designer GPCR
Authors: Tatsumi Manae , Cruz Christian , Kamakura Nozomi , Kuwabara Riku , Nakamura Gaku , Ikuta Tatsuya , Abrol Ravinder , Inoue Asuka 
Published: May 2024

AbstractG-protein-coupled receptors (GPCRs) transduce diverse signals into the cell by coupling to one or several Gα subtypes. Of the 16 Gα subtypes in human cells, Gα12 and Gα13 belong to the G12 subfamily and are reported to be functionally different. Notably, certain GPCRs display selective coupling to either Gα12 or Gα13, highlighting their significance in various cellular contexts. However, the structural basis underlying this selectivity remains unclear. Here, using a Gα12-coupled designer receptor exclusively activated by designer drugs (DREADD; G12D) as a model system, we identified residues in the α5 helix and the receptor that collaboratively determine Gα12-vs-Gα13 selectivity. Residue-swapping experiments showed that G12D distinguishes differences between Gα12 and Gα13 in the positions G.H5.09 and G.H5.23 in the α5 helix. Molecular dynamics simulations observed that I378G.H5.23 in Gα12 interacts with N1032.39, S1693.53 and Y17634.53 in G12D, while H364G.H5.09 in Gα12 interact with Q2645.71 in G12D. Screening of mutations at these positions in G12D identified G12D mutants that enhanced coupling with Gα12 and to an even greater extent with Gα13. Combined mutations, most notably the dual Y17634.53H and Q2645.71R mutant, further enhanced Gα12/13 coupling, thereby serving as a potential Gα12/13-DREADD. Such novel Gα12/13-DREADD may be useful in future efforts to develop drugs that target Gα12/13 signaling as well as to identify their therapeutic indications.
Layout analysis of the RCEP international airline network based on hub identification using improved contribution matrix
Authors: Yang Wendong , Chi Yulin , Huang Yining , Wei Wenbin , Xu Zhengjia 
Published: May 2024

AbstractThe signing of the Regional Comprehensive Economic Partnership agreement brings new opportunities for the development of international air transportation. Faced with fierce competition, it is worth studying how hub airports should enhance competitiveness, and how low-cost carriers and full-service carriers should optimize the RCEP international airline network layout for better development. Aiming at providing suggestions for the development of hub airports, low-cost and full-service carriers in the RCEP international airline network, this paper identifies the hub airports, analyzes the layout of the RCEP international airline network, and the multi-layered characteristics based on an improved contribution matrix using data from 2010 to 2019 collected from the Official Airline Guide (OAG). This method comprehensively considers attributes of hub airports and the multi-layered characteristics of the airports and routes. The layout analysis indicates that the RCEP international transportation market presents a more open environment for competition and cooperation where base carriers are often the biggest supporters of hub construction. The multi-layered characteristics analysis reveals that low-cost carriers contribute more towards opening up new RCEP routes than full-service carriers. It is advised that carriers newly entering the RCEP international aviation transportation market and low-cost carriers dedicate to establishing new routes around their hub airports to monopolize this market and enhance their market share, whilst full-service carriers consolidate existing routes and increase route density to achieve economic benefits.
Diagnostic utility of clinicodemographic, biochemical and metabolite variables to identify viable pregnancies in a symptomatic cohort during early gestation
Authors: Hill Christopher J. , Phelan Marie M. , Dutton Philip J. , Busuulwa Paula , Maclean Alison , Davison Andrew S. , Drury Josephine A. , Tempest Nicola , Horne Andrew W. , Gutiérrez Eva Caamaño , Hapangama Dharani K. 
Published: May 2024

AbstractA significant number of pregnancies are lost in the first trimester and 1–2% are ectopic pregnancies (EPs). Early pregnancy loss in general can cause significant morbidity with bleeding or infection, while EPs are the leading cause of maternal mortality in the first trimester. Symptoms of pregnancy loss and EP are very similar (including pain and bleeding); however, these symptoms are also common in live normally sited pregnancies (LNSP). To date, no biomarkers have been identified to differentiate LNSP from pregnancies that will not progress beyond early gestation (non-viable or EPs), defined together as combined adverse outcomes (CAO). In this study, we present a novel machine learning pipeline to create prediction models that identify a composite biomarker to differentiate LNSP from CAO in symptomatic women. This prospective cohort study included 370 participants. A single blood sample was prospectively collected from participants on first emergency presentation prior to final clinical diagnosis of pregnancy outcome: LNSP, miscarriage, pregnancy of unknown location (PUL) or tubal EP (tEP). Miscarriage, PUL and tEP were grouped together into a CAO group. Human chorionic gonadotrophin β (β-hCG) and progesterone concentrations were measured in plasma. Serum samples were subjected to untargeted metabolomic profiling. The cohort was randomly split into train and validation data sets, with the train data set subjected to variable selection. Nine metabolite signals were identified as key discriminators of LNSP versus CAO. Random forest models were constructed using stable metabolite signals alone, or in combination with plasma hormone concentrations and demographic data. When comparing LNSP with CAO, a model with stable metabolite signals only demonstrated a modest predictive accuracy (0.68), which was comparable to a model of β-hCG and progesterone (0.71). The best model for LNSP prediction comprised stable metabolite signals and hormone concentrations (accuracy = 0.79). In conclusion, serum metabolite levels and biochemical markers from a single blood sample possess modest predictive utility in differentiating LNSP from CAO pregnancies upon first presentation, which is improved by variable selection and combination using machine learning. A diagnostic test to confirm LNSP and thus exclude pregnancies affecting maternal morbidity and potentially life-threatening outcomes would be invaluable in emergency situations.
Laser weeding: opportunities and challenges for couch grass (Elymus repens (L.) Gould) control
Authors: Andreasen Christian , Vlassi Eleni , Salehan Najmeh 
Published: May 2024

AbstractLaser weeding may contribute to less dependency on herbicides and soil tillage. Several research and commercial projects are underway to develop robots equipped with lasers to control weeds. Artificial intelligence can be used to locate and identify weed plants, and mirrors can be used to direct a laser beam towards the target to kill it with heat. Unlike chemical and mechanical weed control, laser weeding only exposes a tiny part of the field for treatment. Laser weeding leaves behind only ashes from the burned plants and does not disturb the soil. Therefore, it is an eco-friendly method to control weed seedlings. However, perennial weeds regrow from the belowground parts after the laser destroys the aerial shoots. Depletion of the belowground parts for resources might be possible if the laser continuously kills new shoots, but it may require many laser treatments. We studied how laser could be used to destroy the widespread and aggressive perennial weed Elymus repens after the rhizomes were cut into fragments. Plants were killed with even small dosages of laser energy and stopped regrowing. Generally, the highest efficacy was achieved when the plants from small rhizomes were treated at the 3-leaf stage.
Introduced species shed friends as well as enemies
Authors: Xirocostas Zoe A. , Ollerton Jeff , Peco Begoña , Slavich Eve , Bonser Stephen P. , Pärtel Meelis , Raghu S. , Moles Angela T. 
Published: May 2024

AbstractMany studies seeking to understand the success of biological invasions focus on species’ escape from negative interactions, such as damage from herbivores, pathogens, or predators in their introduced range (enemy release). However, much less work has been done to assess the possibility that introduced species might shed mutualists such as pollinators, seed dispersers, and mycorrhizae when they are transported to a new range. We ran a cross-continental field study and found that plants were being visited by 2.6 times more potential pollinators with 1.8 times greater richness in their native range than in their introduced range. Understanding both the positive and negative consequences of introduction to a new range can help us predict, monitor, and manage future invasion events.
Enhancing gain and isolation of a quad-element MIMO antenna array design for 5G sub-6 GHz applications assisted with characteristic mode analysis
Authors: Khan Rabia , Sethi Waleed Tariq , Malik Waqar Ahmad , Jan Latif , Tahseen Muhammad M. , Almuhlafi Ali M. , Himdi Mohamed 
Published: May 2024

AbstractThis paper presents a novel quad-element array with multiple inputs and multiple outputs (MIMO) designed for 5th generation sub-6 GHz applications. The MIMO system achieves a wide impedance bandwidth, high gain, and high isolation among its components, representing significant advancements in sub-6 GHz antenna applications. The single element, an elliptical resonator with a circular slot, is fed by a 50 Ω microstrip feedline, achieves a broad characteristic bandwidth from 3.7 to 5.7 GHz with a resonant frequency of 4.33 GHz and a gain of 1.81 dBi. Characteristic Mode Analysis (CMA) was employed to elucidate the evolution phases of this design. The quad-element MIMO antenna array maintains a compact size and broadband characteristics by arranging mirrored elements on the same ground plane. Implemented on a cost-effective FR-4 substrate measuring 44 × 44 × 1.6 mm3, the recommended MIMO antenna array, enhanced with a partial ground plane and due to the introduction of a vertical strip, a high isolation of − 38.53 dB is achieved between MIMO components along with a realized gain of 3.01 dBi and a radiation efficiency of 71% in the 5G sub-6 GHz band. Noteworthy properties include high isolation, diversity gain (DG), and envelope correlation coefficient (ECC), verifying the appropriateness of the suggested MIMO scheme for 5G transmission and reception in sub-6 GHz applications.
The impact of diabetes status on pain and physical function following total joint arthroplasty for hip and knee osteoarthritis: variation by sex and body mass index
Authors: Power J. Denise , Perruccio Anthony V. , Canizares Mayilee , Davey J. Roderick , Gandhi Rajiv , Mahomed Nizar N. , Syed Khalid , Veillette Christian , Rampersaud Y. Raja 
Published: May 2024

AbstractFew studies have examined diabetes impact on total joint arthroplasty (TJA) outcomes, with variable findings. We investigated the association between diabetes and post-TJA physical function and pain, examining whether diabetes impact differs by sex and BMI. Patient sample completed questionnaires within 3 months prior to hip or knee TJA for osteoarthritis (OA) and 1-year post-surgery. Surgical ‘non-response’ was defined as < 30% improvement in Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) pain and physical function at 1-year. Two adjusted logistic regression models were estimated: (1) excluding, (2) including an interaction between diabetes, sex and BMI. The sample (626 hip, 754 knee) was 54.9% female, had mean BMI of 30.1, 13.0% reported diabetes. In adjusted models excluding an interaction, diabetes was not associated with non-response. However, a significant 3-way interaction (physical function: p = 0.003; pain: p = 0.006) between diabetes, sex, and BMI was found and was associated with non-response: non-response probability increased with increasing BMI in men with diabetes, but decreased with increasing BMI in women in diabetes. Findings suggest uncertainty in diabetes impact may be due to differential impacts by sex and BMI. A simple consideration of diabetes as present vs. absent may not be sufficient, with implications for the large TJA population.
Deep ocean hydrographic variability estimated from distributed geodetic sensor arrays off northern Chile
Authors: Jegen Anna , Lange Dietrich , Karstensen Johannes , Pizarro Oscar , Kopp Heidrun 
Published: May 2024

AbstractObservations of spatio-temporal variability of the deep ocean are rare and little is known about occurrence of deep ocean mesoscale dynamics. Here, we make use of 2.5 years of time series data from three distributed sensor arrays, which acquired high-resolution temperature, pressure and sound speed data of the bottom layer offshore northern Chile. Estimating salinity and density from the direct observations enable access to the full spectrum of hydrographic variability from a multi-hourly to annual time scale and with average inter-station distances of less than 1 km. Analyses revealed interannual warming over the continental slope of 0.002 °C yr−1–0.003 °C yr−1, and could trace periodic hydrographic anomalies, likely related to coastal-trapped waves, as far as to the lower continental slope. A concurrent change in the shape of the warm anomalies and the rate of deep-sea warming that occurs with the crossing of the deep-sea trench suggests that the abyssal part of the eastern boundary current system off Chile does not extend past the deep sea trench. Furthermore, the comparison of anomaly timing and shape in between stations implies southwards flow over the mid to lower continental slope, centred closer to the trench.
Production of sounds by squirrelfish during symbiotic relationships with cleaner wrasses
Authors: Banse Marine , Lecchini David , Sabbe Justine , Hanssen Noémie , Donaldson Terry , Iwankow Guillaume , Lagant Anthony , Parmentier Eric 
Published: May 2024

AbstractExamples of symbiotic relationships often include cleaning mutualisms, typically involving interactions between cleaner fish and other fish, called the clients. While these cleaners can cooperate by removing ectoparasites from their clients, they can also deceive by feeding on client mucus, a behavior usually referred to as “cheating behavior” that often leads to a discernible jolt from the client fish. Despite extensive studies of these interactions, most research has focused on the visual aspects of the communication. In this study, we aimed to explore the role of acoustic communication in the mutualistic relationship between cleaner fishes and nine holocentrid client species across four regions of the Indo-Pacific Ocean: French Polynesia, Guam, Seychelles, and the Philippines. Video cameras coupled with hydrophones were positioned at various locations on reefs housing Holocentridae fish to observe their acoustic behaviors during interactions. Our results indicate that all nine species of holocentrids can use acoustic signals to communicate to cleaner fish their refusal of the symbiotic interaction or their desire to terminate the cooperation. These sounds were predominantly observed during agonistic behavior and seem to support visual cues from the client. This study provides a novel example of acoustic communication during a symbiotic relationship in teleosts. Interestingly, these vocalizations often lacked a distinct pattern or structure. This contrasts with numerous other interspecific communication systems where clear and distinguishable signals are essential. This absence of a clear acoustic pattern may be because they are used in interspecific interactions to support visual behavior with no selective pressure for developing specific calls required in conspecific recognition. The different sound types produced could also be correlated with the severity of the client response. There is a need for further research into the effects of acoustic behaviors on the quality and dynamics of these mutualistic interactions.
Lipase-mediated detoxification of host-derived antimicrobial fatty acids by Staphylococcus aureus
Authors: Kengmo Tchoupa Arnaud , Elsherbini Ahmed M. A. , Camus Justine , Fu Xiaoqing , Hu Xuanheng , Ghaneme Oumayma , Seibert Lea , Lebtig Marco , Böcker Marieke A. , Horlbeck Anima , Lambidis Stilianos P. , Schittek Birgit , Kretschmer Dorothee , Lämmerhofer Michael , Peschel Andreas 
Published: May 2024

AbstractLong-chain fatty acids with antimicrobial properties are abundant on the skin and mucosal surfaces, where they are essential to restrict the proliferation of opportunistic pathogens such as Staphylococcus aureus. These antimicrobial fatty acids (AFAs) elicit bacterial adaptation strategies, which have yet to be fully elucidated. Characterizing the pervasive mechanisms used by S. aureus to resist AFAs could open new avenues to prevent pathogen colonization. Here, we identify the S. aureus lipase Lip2 as a novel resistance factor against AFAs. Lip2 detoxifies AFAs via esterification with cholesterol. This is reminiscent of the activity of the fatty acid-modifying enzyme (FAME), whose identity has remained elusive for over three decades. In vitro, Lip2-dependent AFA-detoxification was apparent during planktonic growth and biofilm formation. Our genomic analysis revealed that prophage-mediated inactivation of Lip2 was rare in blood, nose, and skin strains, suggesting a particularly important role of Lip2 for host – microbe interactions. In a mouse model of S. aureus skin colonization, bacteria were protected from sapienic acid (a human-specific AFA) in a cholesterol- and lipase-dependent manner. These results suggest Lip2 is the long-sought FAME that exquisitely manipulates environmental lipids to promote bacterial growth in otherwise inhospitable niches.
A millennium-long perspective on recent groundwater changes in the Iberian Peninsula
Authors: Diodato Nazzareno , Seim Andrea , Ljungqvist Fredrik Charpentier , Bellocchi Gianni 
Published: May 2024

AbstractGroundwater dynamics are often overlooked within historical climatology because of their complexity and the influence of multiple factors. This study presents a groundwater model for Spain, using an existing tree-ring based summer drought reconstruction to estimate the groundwater depth in Castile and León (northwestern Spain) over the 1056–2020 CE period. Spanish groundwater volume fluctuations are found to be associated with quasi-decadal variations in the North Atlantic and Pacific Oceans. The reconstructed annual groundwater depth shows significant oscillations around a mean value of 123 m. Changes in groundwater depths include a wet medieval period ( ~ 1056–1200 CE), recurring megadroughts during parts of the Little Ice Age (~1471–1600 CE), and unprecedentedly large variations during recent decades. Aligning with previous studies for the Iberian Peninsula, our new modelling approach highlights the need to enhance groundwater resilience in anticipation of potentially worsening future drought trends across the Mediterranean.
PKM2 functions as a histidine kinase to phosphorylate PGAM1 and increase glycolysis shunts in cancer
Authors: Wang Yang , Shu Hengyao , Qu Yanzhao , Jin Xin , Liu Jia , Peng Wanting , Wang Lihua , Hao Miao , Xia Mingjie , Zhao Zhexuan , Dong Kejian , Di Yao , Tian Miaomiao , Hao Fengqi , Xia Chaoyi , Zhang Wenxia , Ba Xueqing , Feng Yunpeng , Wei Min 
Published: May 2024

AbstractPhosphoglycerate mutase 1 (PGAM1) is a key node enzyme that diverts the metabolic reactions from glycolysis into its shunts to support macromolecule biosynthesis for rapid and sustainable cell proliferation. It is prevalent that PGAM1 activity is upregulated in various tumors; however, the underlying mechanism remains unclear. Here, we unveil that pyruvate kinase M2 (PKM2) moonlights as a histidine kinase in a phosphoenolpyruvate (PEP)-dependent manner to catalyze PGAM1 H11 phosphorylation, that is essential for PGAM1 activity. Moreover, monomeric and dimeric but not tetrameric PKM2 are efficient to phosphorylate and activate PGAM1. In response to epidermal growth factor signaling, Src-catalyzed PGAM1 Y119 phosphorylation is a prerequisite for PKM2 binding and the subsequent PGAM1 H11 phosphorylation, which constitutes a discrepancy between tumor and normal cells. A PGAM1-derived pY119-containing cell-permeable peptide or Y119 mutation disrupts the interaction of PGAM1 with PKM2 and PGAM1 H11 phosphorylation, dampening the glycolysis shunts and tumor growth. Together, these results identify a function of PKM2 as a histidine kinase, and illustrate the importance of enzyme crosstalk as a regulatory mode during metabolic reprogramming and tumorigenesis.
Organometallic copper(ii) complex of meso–meso N-methyl N-confused pyrrole-bridged doubly N-methyl N-confused hexaphyrin
Authors: Jana Manik , Blasco Daniel , Sundholm Dage , Rath Harapriya 
Published: May 2024

Dual macrocyclic π-conjugation in the paramagnetic Cu(II) organometallic complex of meso-meso N-methyl N-confused pyrrole bridged highly stable nonaromatic doubly N-methyl N-confused hexaphyrin analogue .