bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exosomes isolated from TNF‐α‐treated bone marrow mesenchymal stem cells ameliorate pelvic floor dysfunction in rats
Authors: Zhou Chenchen , Wu Yuelin , Wan Sheng , Lou Liqun , Gu Shengyi , Peng Jing , Zhao Shifeng , Hua Xiaolin 
Published: June 2024

AbstractExosomes derived from bone marrow‐derived mesenchymal stem cells (BMSCs) can alleviate the symptoms of pelvic floor dysfunction (PFD) in rats. However, the potential therapeutical effects of exosomes derived from BMSCs treated with tumour necrosis factor (TNF)‐α on the symptoms of PFD in rats are unknown. Exosomes extracted from BMSCs treated with or without TNF‐α were applied to treat PFD rats. Our findings revealed a significant elevation in interleukin (IL)‐6 and TNF‐α, and matrix metalloproteinase‐2 (MMP2) levels in the vaginal wall tissues of patients with pelvic organ prolapse (POP) compared with the control group. Daily administration of exosomes derived from BMSCs, treated either with or without TNF‐α (referred to as Exo and TNF‐Exo), resulted in increased void volume and bladder void pressure, along with reduced peak bladder pressure and leak point pressure in PFD rats. Notably, TNF‐Exo treatment demonstrated superior efficacy in restoring void volume, bladder void pressure and the mentioned parameters compared with Exo treatment. Importantly, TNF‐Exo exhibited greater potency than Exo in restoring the levels of multiple proteins (Elastin, Collagen I, Collagen III, IL‐6, TNF‐α and MMP2) in the anterior vaginal walls of PFD rats. The application of exosomes derived from TNF‐α‐treated BMSCs holds promise as a novel therapeutic approach for treating PFD.
Conveying “Fun” Through Communicative Moves of Facial Expressions: Investigating the Theory of Affective Pragmatics
Authors: Namba Shushi , Fukuda Haruka , Nakashima Miharu , Takeuchi Mone , Yamamoto Reika , Yoshikawa Kota 
Published: June 2024

AbstractAccording to the theory of affective pragmatics, emotional expressions synchronously convey information of the following four types: expressing the expressor's emotions, committing the expressor to a future course of action, representing world circumstances, and directing other people's behavior. Nevertheless, few reports have described studies examining prioritization of the conveyed information. The present study was conducted to explore how information is conveyed by facial expressions of fun in the context of the presence or absence of another person. Results show that expression of the internal state (fun) is conveyed primarily in comparison to other information, but it is weaker in the state of being with a stranger than in the state of being alone or with a friend. Although directing other people's behavior is the least conveyed information in all conditions, the presence of another person amplified the intensity of directing other people's behavior. Overall, these findings provide new evidence supporting the theory related to emotional expressions.
Environmentalism and Christian Values in Hong Kong: The Potential Influences of Stewardship, Justice, Love, and Church Environmental Education
Authors: Ho Mei Yan , Hui Tin Yan , Chan Janet K. Y. 
Published: June 2024

AbstractReligious attributes affecting Christians’ environmentalism have received considerable attention since the publication of Lynn White's thesis in 1967, which accused Christianity as the root cause of the ecological crisis. This study aims to assess the relationship between Christianity and environmentalism in Hong Kong, an Asian city where Christianity has been introduced for just more than two centuries. In general, Hong Kong Christians had a stronger willingness to sacrifice for the environment as compared to non‐Christians. Both stewardship worldview and Christian justice and love were identified as the main drivers for environmentalism in Christians, which can be further improved via church environmental education. Our findings provide a particular example to broaden the diversity of the Christianity‐environmentalism nexus and highlight the functional role of stewardship worldview in the Hong Kong context. However, we also call for stronger environmental education in Hong Kong's church to bridge the willingness‐behavior gap among local Christians.
Biomechanical Effects on the Prostheses and Vertebrae of Three‐Level Hybrid Surgery: A Finite Element Study
Authors: Huang Kangkang , Wang Qian , Rong Xin , Wu Tingkui , Ding Chen , Meng Yang , Yin Wanhong , Liu Hao , Wang Beiyu 
Published: June 2024

PurposeThree‐level hybrid surgery (HS) consisting of cervical disc arthroplasty (CDA) and anterior cervical discectomy and fusion (ACDF) has been partly used for the treatment of multi‐level cervical degenerative disc disease (CDDD). The complications related to the implants and the collapse of the surgical vertebral bodies had been reported in multi‐level anterior cervical spine surgery. Thus, this study aimed to explore the biomechanical effects on the prostheses and vertebrae in three‐level HS.MethodsA FE model of cervical spine (C0‐T1) was constructed. Five surgical models were developed. They were FAF model (ACDF‐CDA‐ACDF), AFA model (CDA‐ACDF‐CDA), FFF model (three‐level ACDF), SF model (single‐level ACDF), and SA model (single‐level CDA). A 75‐N follower load and 1.0‐N·m moment was applied to produce flexion, extension, lateral bending, and axial rotation.ResultsCompared with the intact model, the range of motion (ROM) of total cervical spine in FAF model decreased by 34.54%, 54.48%, 31.76%, and 27.14%, respectively, in flexion, extension, lateral bending, and axial rotation, which were lower than those in FFF model and higher than those in AFA model. The ROMs of CDA segments in FAF and AFA models were similar to the intact model and SA model. Compared with the intact model, the ROMs at C3/4 segment in FFF model increased from 5.71% to 7.85%, and increased from 5.31% to 6.81% at C7/T1 segment, following by FAF model, then the FAF model. The maximum interface pressures of the Prestige‐LP in FAF model were similar to SA model, however the corresponding values were increased in AFA model. The maximum interface pressures of the Zero‐P were increased in FAF and AFA model compared with those in SF and FFF models. The stress was mainly distributed on the screws. In AFA model, the maximum pressures of the ball and trough articulation in superior and inferior Prestige‐LP were all increased compared with those in SA and FAF model. In FFF model, the maximum pressures of the vertebrae were higher than those in other models. The stress was mainly distributed on the anterior area of the vertebral bodies.ConclusionsHS seemed to be more suitable than ACDF for the surgical treatment of three‐level CDDD in consideration of the biomechanical effects, especially for the two‐level CDA and one‐level ACDF construct. But a more appropriate CDA prosthesis should be explored in the future.
Six‐year trend of subsequent allergic diseases following Kawasaki disease and its clinical implications: A population‐based matched cohort study of 34,712 patients
Authors: Kim Dongeon , Shin Ji Seong , Kim Sin Young , Kim Jihye 
Published: June 2024

AbstractBackgroundIt has been suggested that allergic diseases may increase after Kawasaki disease (KD). We aimed to analyze the temporal patterns of allergic disease incidence after KD.MethodsA nationwide population‐based matched cohort study was conducted using data from the Korean National Health Insurance claims database. Patients aged <5 years diagnosed with KD and their 1:3 propensity score‐matched controls were included. Three cohorts were established: Cohort A, patients with allergies; Cohort B, patients without allergies; and Cohort C, patients without allergies, but excluding patients with birth history and underlying medical conditions. Cumulative incidence rates (%) and associated hospital visits for allergic rhinitis, atopic dermatitis, urticaria, and asthma were compared between the cases and controls during the 6‐year follow‐up period.ResultsThe study population comprised 8678 patients diagnosed with KD and 26,034 controls. In Cohort A, although initially, there were intergroup differences in the number of hospital visits for certain allergic diseases, these differences were inconsistent and varied depending on the type of allergic disease. Over time, the differences narrowed, and by the sixth year, the gap had decreased significantly. In Cohorts B and C, the initial incidence rates of the four allergic diseases and associated hospital visits were lower in patients with KD as compared to controls. However, with a faster rate of increase, the incidence rates and number of hospital visits eventually surpassed those of the controls.ConclusionsThe pattern of delayed increase in cumulative incidence rates and hospital visits for allergic diseases after KD suggests the possibility of a shared genetic or immunologic susceptibility between KD and allergic diseases, which becomes evident over time, rather than a direct influence of KD resulting in allergic diseases.
NbPTR1 confers resistance against Pseudomonas syringae pv. actinidiae in kiwifruit
Authors: Yeh Shin‐Mei , Yoon Minsoo , Scott Sidney , Chatterjee Abhishek , Hemara Lauren M. , Chen Ronan K. Y. , Wang Tianchi , Templeton Kerry , Rikkerink Erik H. A. , Jayaraman Jay , Brendolise Cyril 
Published: June 2024

AbstractPseudomonas syringae pv. actinidiae biovar 3 (Psa3) causes a devastating canker disease in yellow‐fleshed kiwifruit (Actinidia chinensis). The effector HopZ5, which is present in all isolates of Psa3 causing global outbreaks of pandemic kiwifruit canker disease, triggers immunity in Nicotiana benthamiana and is not recognised in susceptible A. chinensis cultivars. In a search for N. benthamiana nonhost resistance genes against HopZ5, we found that the nucleotide‐binding leucine‐rich repeat receptor NbPTR1 recognised HopZ5. RPM1‐interacting protein 4 orthologues from N. benthamiana and A. chinensis formed a complex with NbPTR1 and HopZ5 activity was able to disrupt this interaction. No functional orthologues of NbPTR1 were found in A. chinensis. NbPTR1 transformed into Psa3‐susceptible A. chinensis var. chinensis ‘Hort16A’ plants introduced HopZ5‐specific resistance against Psa3. Altogether, this study suggested that expressing NbPTR1 in Psa3‐susceptible kiwifruit is a viable approach to acquiring resistance to Psa3 and it provides valuable information for engineering resistance in otherwise susceptible kiwifruit genotypes.
Epidermal nevi and epidermolytic hyperkeratosis: A review of cases, highlighting indications for biopsy and genetics referral
Authors: Nelson Jessie M. , Isaac Jacqueline M. , Mervak Julie E. , Mancuso Jennifer B. , Chan May P. , Arreola Amanda , Cha Kelly B. 
Published: June 2024

AbstractEpidermal nevi are common benign cutaneous hamartomas that may rarely demonstrate histopathologic evidence of epidermolytic hyperkeratosis (EHK), representing cutaneous mosaicism for pathogenic keratin variants. Rarely, individuals with linear epidermal nevi transmit to their children the inherited form of EHK, also known as epidermolytic ichthyosis, characterized by generalized erythema, blistering, and scaling at birth evolving to widespread hyperkeratosis. We present an updated review of reported cases of linear epidermal nevi with EHK exhibiting transmission of epidermolytic ichthyosis to guide important considerations in the care of individuals with epidermal nevi. Clinical characteristics of linear epidermal nevi do not reliably predict the presence of EHK. All reported cases of transmission to offspring have occurred in individuals with linear epidermal nevi involving more than one anatomic area suggesting increased reproductive risk with involvement of two or more anatomic sites. Therefore, genetics consultation is recommended for these individuals with biopsy‐confirmed EHK. For individuals with smaller areas of epidermal nevus involvement, the implications are less well known, though genetics consultation may still be considered for those interested in further discussion of general reproductive risk.
Genome‐wide identification and characterization of SNW/SKIP domain‐containing proteins in plants
Authors: Silva S. A. , Bezerra V. B. F. , Teixeira F. C. , Roque E. M. S. , do Nascimento J. I. B. , Aguiar A. M. , de Carvalho H. H. , Alves M. S. 
Published: June 2024

Abstract

Sessile organisms, such as plants, developed various ways to sense and respond to external and internal stimuli to maximize their fitness through evolutionary time. Transcripts and protein regulation are, among many, the main mechanisms that plants use to respond to environmental changes. SKIP protein is one such, presenting an SNKW interacting domain, which is highly conserved among eukaryotes, where SKI interacting protein acts in regulating key processes.

In the present work, many bioinformatics tools, such as phylogenetic relationships, gene structure, physical–chemical properties, conserved motifs, prediction of regulatory cis‐elements, chromosomal localization, and protein–protein interaction network, were used to better understand the genome‐wide SNW/SKIP domain‐containing proteins.

In total, 28 proteins containing the SNW/SKIP domain were identified in different plant species, including plants of agronomic interest. Two main protein clusters were formed in phylogenetic analysis, and gene structure analysis revealed that, in general, the coding region had no introns. Also, expression of these genes is possibly induced by abiotic stress stimuli. Primary structure analysis of the proteins revealed the existence of an evolutionarily conserved functional unit. But physicochemical properties show that proteins containing the SNW/SKIP domain are commonly unstable under in vivo conditions.

In addition, the protein network, demonstrated that SKIP homologues could act by modulating plant fitness through gene expression regulation at the transcriptional and post‐transcriptional levels. This could be corroborated by the expression number of gene copies of SKIP proteins in many species, highlighting it's crucial role in plant development and tolerance through the course of evolution.
The efficacy and safety of autologous epidermal cell suspensions for re‐epithelialization of skin lesions: A systematic review and meta‐analysis of randomized trials
Authors: Lou Jiaqi , Xiang Ziyi , Fan Youfen , Song Jingyao , Huang Neng , Li Jiliang , Jin Guoying , Cui Shengyong 
Published: June 2024

AbstractBackgroundSuccessful usage of autologous skin cell suspension (ASCS) has been demonstrated in some clinical trials. However, its efficacy and safety have not been verified. This latest systematic review and meta‐analysis aim to examine the effects of autologous epidermal cell suspensions in re‐epithelialization of skin lesions.MethodsRelevant articles were retrieved from PubMed, Embase, Cochrane Database, Web of Science, International Clinical Trials Registry Platform, China National Knowledge Infrastructureris, VIP Database for Chinese Technical Periodicals and Wanfang database. The primary output measure was the healing time, and the secondary outputs were effective rate, size of donor site for treatment, size of study treatment area, operation time, pain scores, repigmentation, complications, scar scale scores and satisfaction scores. Data were pooled and expressed as relative risk (RR), mean difference (MD) and standardized mean difference (SMD) with a 95% confidence interval (CI).ResultsThirty‐one studies were included in this systematic review and meta‐analysis, with 914 patients who received autologous epidermal cell suspensions (treatment group) and 883 patients who received standard care or placebo (control group). The pooled data from all included studies demonstrated that the treatment group has significantly reduced healing time (SMD = −0.86; 95% CI: −1.59–0.14; p = 0.02, I2 = 95%), size of donar site for treatment (MD = −115.41; 95% CI: −128.74–102.09; p<0.001, I2 = 89%), operation time (MD = 25.35; 95% CI: 23.42–27.29; p<0.001, I2 = 100%), pain scores (SMD = −1.88; 95% CI: −2.86–0.90; p = 0.0002, I2 = 89%) and complications (RR = 0.59; 95% CI: 0.36–0.96; p = 0.03, I2 = 66%), as well as significantly increased effective rate (RR = 1.20; 95% CI: 1.01–1.42; p = 0.04, I2 = 77%). There were no significant differences in the size of study treatment area, repigmentation, scar scale scores and satisfaction scores between the two groups.ConclusionOur meta‐analysis showed that autologous epidermal cell suspensions is beneficial for re‐epithelialization of skin lesions as they significantly reduce the healing time, size of donar site for treatment, operation time, pain scores and complications, as well as increased effective rate. However, this intervention has minimal impact on size of treatment area, repigmentation, scar scale scores and satisfaction scores.
Film-Cooling Effectiveness on a Turbine Blade Platform With Various Hole Shapes and Layouts
Authors: Hu Jia-Jun , An Bai-Tao 
Published: June 2024

Abstract
This paper reports a film-cooling effectiveness experiment on a turbine blade platform that combines two film hole shapes with three layouts. A linear cascade using the pressure-sensitive paint (PSP) technique was employed to measure the adiabatic film effectiveness and discharge coefficients. Three film hole layouts, including two double-row layouts and one dispersed layout, were designed based on the platform configurations. One double-row layout arranges both rows of holes on the pressure side. Another double-row layout arranges one row on the pressure side and one row on the suction side. The dispersed layout was designed with streamwise multirows using the same number of holes. A fan-shaped hole and a diffusion slot hole were tested and compared. The experiments were conducted at a mainstream Reynolds number of 7 × 105, a mainstream turbulence intensity of 3.6%, and a coolant-to-mainstream density ratio of 1.5. The blowing ratio ranged from 0.5 to 2.5. The results demonstrated that regardless of the hole shape, the dispersed layout performed better than the two double-row layouts. However, the effects of the layout on the film effectiveness and discharge coefficients are smaller for the diffusion slot hole. In the three layouts, the film effectiveness of the diffusion slot holes is remarkably greater than that of the fan-shaped holes, and the superiority increases as the blowing ratio increases. In contrast, the superiority of the diffusion slot hole in double-row layouts surpasses that of a dispersed layout.
Characterization of antimalarial activity of artemisinin-based hybrid drugs
Authors: Quadros Helenita Costa , Herrmann Lars , Manaranche Jeanne , Paloque Lucie , Borges-Silva Mariana C. , Dziwornu Godwin Akpeko , D'Alessandro Sarah , Chibale Kelly , Basilico Nicoletta , Benoit-Vical Françoise , Tsogoeva Svetlana B. , Moreira Diogo Rodrigo M. 
Published: June 2024

ABSTRACT

In response to the spread of artemisinin (ART) resistance, ART-based hybrid drugs were developed, and their activity profile was characterized against drug-sensitive and drug-resistant
Plasmodium falciparum
parasites. Two hybrids were found to display parasite growth reduction, stage-specificity, speed of activity, additivity of activity in drug combinations, and stability in hepatic microsomes of similar levels to those displayed by dihydroartemisinin (DHA). Conversely, the rate of chemical homolysis of the peroxide bonds is slower in hybrids than in DHA. From a mechanistic perspective, heme plays a central role in the chemical homolysis of peroxide, inhibiting heme detoxification and disrupting parasite heme redox homeostasis. The hybrid exhibiting slow homolysis of peroxide bonds was more potent in reducing the viability of ART-resistant parasites in a ring-stage survival assay than the hybrid exhibiting fast homolysis. However, both hybrids showed limited activity against ART-induced quiescent parasites in the quiescent-stage survival assay. Our findings are consistent with previous results showing that slow homolysis of peroxide-containing drugs may retain activity against proliferating ART-resistant parasites. However, our data suggest that this property does not overcome the limited activity of peroxides in killing non-proliferating parasites in a quiescent state.
Pseudomonas aeruginosa
producing FIM-1-metallo-β-lactamase: emergence and cross-transmission in a long-term acute care rehabilitation hospital
Authors: Antonelli Alberto , Coppi Marco , Bonaiuto Chiara , Giovacchini Nicla , Grifoni Camilla , Vaggelli Guendalina , Pupillo Roberto , Vannetti Federica , Farese Alberto , Viaggi Bruno , Pollini Simona , Rossolini Gian Maria 
Published: June 2024

ABSTRACT

FIM-1 metallo-β-lactamase was previously detected in sporadic
Pseudomonas aeruginosa
clinical isolates. Here, we report on FIM-1-positive
P. aeruginosa
from two patients who had shared the same ward in a long-term acute care rehabilitation hospital. Whole-genome sequencing analysis revealed close relatedness of these isolates, which belonged to an ST235 sublineage (clade 8/14) different from those previously reported. Results highlighted the occurrence of clonal diversity among FIM-positive strains and the possibility of their cross-transmission in some healthcare settings.
HIV-associated cancers and lymphoproliferative disorders caused by Kaposi sarcoma herpesvirus and Epstein-Barr virus
Authors: Lurain Kathryn A. , Ramaswami Ramya , Krug Laurie T. , Whitby Denise , Ziegelbauer Joseph M. , Wang Hao-Wei , Yarchoan Robert 
Published: June 2024

SUMMARY
Within weeks of the first report of acquired immunodeficiency syndrome (AIDS) in 1981, it was observed that these patients often had Kaposi sarcoma (KS), a hitherto rarely seen skin tumor in the USA. It soon became apparent that AIDS was also associated with an increased incidence of high-grade lymphomas caused by Epstein-Barr virus (EBV). The association of AIDS with KS remained a mystery for more than a decade until Kaposi sarcoma-associated herpesvirus (KSHV) was discovered and found to be the cause of KS. KSHV was subsequently found to cause several other diseases associated with AIDS and human immunodeficiency virus (HIV) infection. People living with HIV/AIDS continue to have an increased incidence of certain cancers, and many of these cancers are caused by EBV and/or KSHV. In this review, we discuss the epidemiology, virology, pathogenesis, clinical manifestations, and treatment of cancers caused by EBV and KSHV in persons living with HIV.
The atypical antipsychotic aripiprazole alters the outcome of disseminated
Candida albicans
infections
Authors: Reitler Parker , Regan Jessica , DeJarnette Christian , Srivastava Ashish , Carnahan Jen , Tucker Katie M. , Meibohm Bernd , Peters Brian M. , Palmer Glen E. 
Published: June 2024

ABSTRACT

Invasive fungal infections impose an enormous clinical, social, and economic burden on humankind. One of the most common species responsible for invasive fungal infections is
Candida albicans
. More than 30% of patients with disseminated candidiasis fail therapy with existing antifungal drugs, including the widely used azole class. We previously identified a collection of 13 medications that antagonize the activity of the azoles on
C. albicans
. Although gain-of-function mutations responsible for antifungal resistance are often associated with reduced fitness and virulence, it is currently unknown how exposure to azole antagonistic drugs impacts
C. albicans
physiology, fitness, or virulence. In this study, we examined how exposure to seven azole antagonists affects
C. albicans
phenotype and capacity to cause disease. Most of the azole antagonists appear to have little impact on fungal growth, morphology, stress tolerance, or gene transcription. However, aripiprazole had a modest impact on
C. albicans
hyphal growth and increased cell wall chitin content. It also aggravated the disseminated
C. albicans
infections in mice. This effect was abrogated in immunosuppressed mice, indicating that it is at least in part dependent upon host immune responses. Collectively, these data provide proof of principle that unanticipated drug-fungus interactions have the potential to influence the incidence and outcomes of invasive fungal disease.