bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence and risk factors associated with precancerous and cancerous cervical lesions among HIV-infected women in University of Gondar specialized comprehensive referral hospital, Northwest Ethiopia: cross-sectional study design
Authors: Worku Elfalet , Yigizaw Getachew , Admassu Robel , Mekonnen Dawit , Gessessa Winta , Tessema Zemenu , Walle Tarkie 
Published: June 2024

Abstract
Background
Cervical cancer is one of the leading causes of death in women worldwide. The majority of the cases are found in developing countries. The increasing risk of cervical cancer prevalence and growing danger of death from cervical cancer and the high occurrence of human papillomavirus (HPV) infection in women who are HIV positive give us the ground to study the prevalence and associated risk factors.

Objective
The study aims to assess the prevalence of cervical cancer screening and associated risk factors among HIV-positive women attending the Adult ART clinic at the University of Gondar Hospital.

Methods
An institution-based cross-sectional study was conducted from March to August 2021, on adult HIV-positive women attending the Adult ART clinic at Gondar University Referral Hospital by phone calling patients per week for six months to complete a total of 2744 HIV-positive patients who were not screened for cervical cancer before. The data were collected using an interviewer-administered questionnaire. Bivariate and multivariable logistic regression analyses were used to determine the presence and the degree of association between dependent and independent variables. In the multivariable logistic analysis, a P-value of < 0.05 and odds ratio with a 95% confidence interval were considered to determine independent predictors for the prevalence of premalignant or malignant cervical lesions among HIV-positive patients.

Result
This study assessed 915 HIV Positive women who were screened for cervical cancer via visual inspection with acetic acid (VIA) as the primary screening tool and found that 24.48% had positive VIA results. Those with VIA-positive cases pathology examination showed 72.4% had abnormal pathology reports (CIN 1/2/3–51.25%, 17.23% cancer & 3.9% CIS), strengthening the finding in many studies that suggest HIV-positive women have a high rate of premalignant lesions.
Association between watching eating shows and unhealthy food consumption in Korean adolescents
Authors: Joo Min Jeong , Kim Dan Bi , Ko Jisu , Lim Jae Hyeok , Park Eun-Cheol , Shin Jaeyong 
Published: June 2024

Abstract
Background
Eating habits formed during adolescence greatly influence the maintenance of health in adulthood. With the recent development of social media and easy access to the Internet, adolescents watch plenty of food videos, particularly Mukbang and Cookbnag(eating show)content. This media genre’s impact on food choices has been covered in several studies; however, studies on unhealthy eating habits directly related to adolescents’ exposure to eating shows are insufficient.

Methods
For this study, we used data from the 18th Korea Youth Risk Behavior Survey conducted in 2022 and finalized 50,451 participants. The extent of exposure to eating show media over the course of a week, as well as the consumption of fast food, sugar-sweetened beverages (SSBs), and high caffeinated beverages within that week were measured through self-reporting questionnaires. We classified the participants into two groups based on their frequency of watching eating shows. A multiple logistic regression analysis was performed to investigate the association between eating show and unhealthy food consumption.

Results
For both males and females, eating show exposure was strongly associated with the consumption of fast food (male: OR:1.37, 95% CI:1.26–1.49; female: OR:1.46, 95% CI:1.36–1.57), SSB (male: OR:1.42, 95% CI:1.26–1.60; female: OR:1.51, 95% CI:1.35–1.70), and high caffeinated beverage (male: OR:1.30, 95% CI:1.23–1.37; female: OR:1.24, 95% CI:1.18–1.31). It was observed that both sexes were more likely to frequently eat unhealthy food than students who did not watch eating shows.

Conclusion
Among Korean adolescents, students exposed to eating shows, which primarily aim to entertain, were more likely to consume fast food, SSBs, and high caffeinated beverages. Therefore, this study's findings suggest that eating show could influence adolescents' food choices, highlighting the need for interest in emerging cultures and corresponding health policies.
The spectrum of factor XI deficiency in Southeast China: four recurrent variants can explain most of the deficiencies
Authors: Zhang Ke , Qin Langyi , Xu Fei , Ye Longying , Wen Mengzhen , Pan Jingye , Yang Lihong , Wang Mingshan , Xie Haixiao 
Published: June 2024

Abstract
Background
Factor XI (FXI) deficiency is an autosomal hemorrhagic disorder characterized by reduced plasma FXI levels. Multiple ancestral variants in the F11 gene have been identified in Ashkenazi Jews and other selected European populations. However, there are few reports of predominant variants in Chinese and/or East Asian populations. The aim of this study is to characterize the genotypes and phenotypes of FXI deficiency and identify the predominant variants.

Results
Of the 41 FXI-deficient patients, 39 exhibited severe FXI defects, considerably more than those with partial defects. The APTT levels showed a negative correlation with FXI activity levels (coefficient=-0.584, P < .001). Only nine patients experienced mild bleeding, including one partially defective patient and eight severely defective patients. The majority of patients were referred for preoperative screenings (n = 22) and checkups (n = 14). Genetic analysis revealed that 90% of the patients had genetic defects, with 2, 16, and 19 cases of heterozygous, homozygous, and compound heterozygous patients, respectively. Seventeen variants were detected in the F11 gene (6 novel), including eleven missense variants, four nonsense variants, and two small deletions scattered throughout the F11. Of the 11 missense variants, six have not yet been studied for in vitro expression. Protein modeling analyses indicated that all of these variants disrupted local structural stability by altering side-chain orientation and hydrogen bonds. Nine variants, consisting of three missense and six null variants, were detected with a frequency of two or more. The highest allele frequency was observed in p.Q281* (21.25%), p.W246* (17.50%), p.Y369* (12.50%), and p.L442Cfs*8 (12.50%). The former two were variants specific to East Asia, while the remaining two were southeast China-specific variants.

Conclusion
Our population-based cohort demonstrated that no correlation between the level of FXI activity and the bleeding severity in FXI deficiency. Additionally, the prevalence of FXI deficiency may have been underestimated. The nonsense p.Q281* was the most common variant in southeast China, suggesting a possible founder effect.
Therapeutic potential of the topical recombinant human interleukin-1 receptor antagonist in guinea pigs with allergic rhinitis
Authors: Li Haibing , Di Chanjuan , Xie Yanbing , Bai Yuexia , Liu Yongxue 
Published: June 2024

Abstract
Background
Recombinant human Interleukin receptor antagonist (rhIL-Ra) can bind to the IL-1 receptor on the cell membrane and reversibly blocks the proinflammatory signaling pathway. However, its effect on allergic rhinitis (AR) and the underlying mechanism remains unknown. This study aims to investigate the efficacy of recombinant human interleukin-1 receptor antagonist (rhIL-1Ra) on AR guinea pigs.

Methods
Guinea pigs were systemically sensitized by intraperitoneal injection and topical intranasal instillation with ovalbumin within 21 days. Animals administrated with saline served as the normal control. The AR animals were randomly divided into the model group and distinct concentrations of rhIL-1Ra and budesonide treatment groups. IL-1β and ovalbumin specific IgE levels were detected by ELISA kits. Nasal mucosa tissues were stained with hematoxylin & eosin (HE) for histological examination.

Results
It was found that the numbers of sneezing and nose rubbing were remarkably reduced in rhIL-1Ra and budesonide-treated guinea pigs. Besides, rhIL-1Ra distinctly alleviated IgE levels in serum and IL-1β levels in nasal mucus, together with decreased exfoliation of epithelial cells, eosinophilic infiltration, tissue edema and vascular dilatation.

Conclusions
rhIL-1Ra is effective in AR guinea pigs and may provide a novel potential choice for AR treatments.
Protein arginine methyltransferase 1 regulates mouse enteroendocrine cell development and homeostasis
Authors: Peng Zhaoyi , Bao Lingyu , Iben James , Wang Shouhong , Shi Bingyin , Shi Yun-Bo 
Published: June 2024

Abstract
Background
The adult intestinal epithelium is a complex, self-renewing tissue composed of specialized cell types with diverse functions. Intestinal stem cells (ISCs) located at the bottom of crypts, where they divide to either self-renew, or move to the transit amplifying zone to divide and differentiate into absorptive and secretory cells as they move along the crypt-villus axis. Enteroendocrine cells (EECs), one type of secretory cells, are the most abundant hormone-producing cells in mammals and involved in the control of energy homeostasis. However, regulation of EEC development and homeostasis is still unclear or controversial. We have previously shown that protein arginine methyltransferase (PRMT) 1, a histone methyltransferase and transcription co-activator, is important for adult intestinal epithelial homeostasis.

Results
To investigate how PRMT1 affects adult intestinal epithelial homeostasis, we performed RNA-Seq on small intestinal crypts of tamoxifen-induced intestinal epithelium-specific PRMT1 knockout and PRMT1fl/fl adult mice. We found that PRMT1fl/fl and PRMT1-deficient small intestinal crypts exhibited markedly different mRNA profiles. Surprisingly, GO terms and KEGG pathway analyses showed that the topmost significantly enriched pathways among the genes upregulated in PRMT1 knockout crypts were associated with EECs. In particular, genes encoding enteroendocrine-specific hormones and transcription factors were upregulated in PRMT1-deficient small intestine. Moreover, a marked increase in the number of EECs was found in the PRMT1 knockout small intestine. Concomitantly, Neurogenin 3-positive enteroendocrine progenitor cells was also increased in the small intestinal crypts of the knockout mice, accompanied by the upregulation of the expression levels of downstream targets of Neurogenin 3, including Neuod1, Pax4, Insm1, in PRMT1-deficient crypts.

Conclusions
Our finding for the first time revealed that the epigenetic enzyme PRMT1 controls mouse enteroendocrine cell development, most likely via inhibition of Neurogenin 3-mediated commitment to EEC lineage. It further suggests a potential role of PRMT1 as a critical transcriptional cofactor in EECs specification and homeostasis to affect metabolism and metabolic diseases.
Nitrogen addition has divergent effects on phosphorus fractions in four types of soils
Authors: Zeng Ping , Zhao Qiong , Hu Jia-yu , Zhang Xiang , Mao Bing , Sun Qing-ye , Wu Wen-ge 
Published: June 2024

Abstract
Background
Globally increasing atmospheric nitrogen (N) deposition has altered soil phosphorus (P) transformations and availability, and thereby influenced structure and function of terrestrial ecosystems. Edaphic characteristics and chemical form of deposited N could be important factors determining impacts of N deposition on soil P transformations, yet the underlying mechanisms remain largely unknown. Objectives of this study were to examine how mineral-N and amino N differently affect P fractions, and identify key soil properties determining N addition impacts on soil P transformations. Considering that amino N is an important component of deposited N and forest soils vary greatly in different regions, the results of present study can guide the management of forests across different soils under ongoing N deposition scenarios.

Methods
We conducted a 60-day laboratory experiment to investigate the effects of N addition (NH4NO3 and glycine) on soil P fractions and related biochemical properties in four representative forest soils (brown, yellow brown, aeolian sandy, and red soils) in China. Glycine and NH4NO3 were separately added at three rates (5, 10 and 20 g N m–2 yr–1).

Results
Firstly, the percent changes in organic P fractions with N addition were significantly greater than changes in inorganic P fractions across all soils. Secondly, the percent changes in P fractions with glycine and NH4NO3 additions were significantly correlated across all soils and treatments. However, glycine addition had significantly greater impacts on organic P fractions than NH4NO3 addition in the aeolian sandy and red soils with low organic carbon content. Thirdly, P fractions responded differently to N addition among the four soils. N-induced changes in microbial biomass and phosphatase activities, pH, exchangeable Ca2+ and Mg2+ contributed differently to the changes in P fractions with N addition in the four soils.

Conclusions
The different responses of P fractions to N addition in the four soils were mainly generated by the differences in extent of microbial N limitation, acid buffering capacity, and cation exchange capacity among the soils. The different impacts of mineral and amino N on soil P fractions can be ascribed to their divergent effects on soil pH, microbial biomass and activities.
A literature review on the drivers and barriers of organic food consumption in China
Authors: Castro Campos Bente , Qi Xue 
Published: June 2024

AbstractResearch interest in organic food consumption has increased significantly worldwide, but a systematic analysis of this phenomenon in China is still lacking. The existing studies in this area are wide-ranging and fragmented, and there is a lack of comprehensive research into the drivers and barriers specific to the Chinese context. Based on a literature review of 26 articles spanning from 2011 to 2021, this article employs the consumption values theory and the innovation resistance theory to discern patterns on the drivers and barriers of organic food consumption in China. The findings show that the most explored drivers include the functional values of healthiness and safety. Within the social value realm, environmental concern and symbolic value emerge as important influences on Chinese consumers. Conditional values shed light on factors such as perceived healthiness and certification/organic labeling. Regarding barriers, an aspect of research less analyzed, the article finds key obstacles being price sensitivity, perceived uncertainty, lack of knowledge or information, lack of reliability, and lack of availability or visibility.
Torpor induces reversible tau hyperphosphorylation and accumulation in mice expressing human tau
Authors: de Veij Mestdagh C. F. , Witte M. E. , Scheper W. , Smit A. B. , Henning R. H. , van Kesteren R. E. 
Published: June 2024

AbstractTau protein hyperphosphorylation and aggregation are key pathological events in neurodegenerative tauopathies such as Alzheimer’s disease. Interestingly, seasonal hibernators show extensive tau hyperphosphorylation during torpor, i.e., the hypothermic and hypometabolic state of hibernation, which is completely reversed during arousal. Torpor-associated mechanisms that reverse tau hyperphosphorylation may be of therapeutic relevance, however, it is currently not known to what extent they apply to human tau. Here we addressed this issue using daily torpor in wildtype mice that express mouse tau (mtau) and in mice that lack mtau expression and instead express human tau (htau). AT8, AT100 and Ser396 immunoblotting and immunohistochemistry were used to assess tau (hyper)phosphorylation at clinically relevant phosphorylation sites. We found that torpor robustly and reversibly increases the levels of phosphorylated tau in both mtau and htau mice. Immunohistochemistry revealed four brain areas that show prominent tau phosphorylation: the hippocampus, posterior parietal cortex, piriform cortex and cortical amygdala. Whereas wildtype mice primarily showed increased levels of diffusely organized hyperphosphorylated tau during torpor, htau mice contained clear somato-dendritic accumulations of AT8 reactivity resembling tau pre-tangles as observed in the Alzheimer brain. Interestingly, AT8-positive accumulations disappeared upon arousal, and tau phosphorylation levels at 24 h after arousal were lower than observed at baseline, suggesting a beneficial effect of torpor-arousal cycles on preexisting hyperphosphorylated tau. In conclusion, daily torpor in mice offers a quick and standardized method to study tau phosphorylation, accumulation and clearance in mouse models relevant for neurodegeneration, as well as opportunities to discover new targets for the treatment of human tauopathies.
Design of enlarged phononic bandgap 2.5D acoustic resonator via active learning and non-gradient optimization
Authors: Ibrahim Syed Muhammad Anas , Park Jungyul 
Published: June 2024

AbstractIdentifying the phononic crystal (PnC) with bandgap is a problematic process because all phononic crystals don’t have bandgap. Predicting the Phononic bandgaps (PnBGs) is a computationally expensive task. Here we explore the potential of machine learning (ML) tools to expedite the prediction and maximize the resonator based PnBG. The Gaussian process regression (GPR) model is trained to learn the relationship between complicated shape and band structure of cavity. Bayesian optimization (BO) derives a new shape by leveraging the fast inference of the trained model, which is updated with the augmentation of newly explored structures to escalate the prediction power over performance expansion through active learning. Artificial intelligence (AI) assisted optimization requires a small number of generations to achieve convergence. The obtained results are validated via experimental measurements.
Raman spectroscopic comparison of zearalenone and its derivatives for non-destructive rapid detection
Authors: Kang Won-Seok , Choi Sung-Wook , Kim Hyo-Sop , Kim Jae-Ho , Lee Jae-Hyeok 
Published: June 2024

AbstractRaman spectroscopy is used to investigate the absorption and dispersion of zearalenone (ZEN) and its derivatives. The C=C stretching vibration modes of ZEN and zearalenol (ZENOL) were appeared at 975–990 cm−1. The C=O vibration mode was present at 1680–1690 cm−1 for ZEN and zearalanone (ZAN), but it was absent for ZENOL and zearalanol (ZANOL) which have –OH group instead of –C=O group in ZEN and ZAN molecular structures. On the basis of this characterization, Raman spectra of specific chemical groups and linkages corresponding to the structural difference of ZEN and its derivatives were identified. These results indicated that Raman spectroscopy can apply for the identification of ZEN and its derivatives and has a potential for the non-destructive rapid detection of these compounds in food.
Gastroenteropancreatic neuroendocrine neoplasms: current development, challenges, and clinical perspectives
Authors: Zhang Xian-Bin , Fan Yi-Bao , Jing Rui , Getu Mikiyas Amare , Chen Wan-Ying , Zhang Wei , Dong Hong-Xia , Dakal Tikam Chand , Hayat Akhtar , Cai Hua-Jun , Ashrafizadeh Milad , Abd El-Aty A. M. , Hacimuftuoglu Ahmet , Liu Peng , Li Tian-Feng , Sethi Gautam , Ahn Kwang Seok , Ertas Yavuz Nuri , Chen Min-Jiang , Ji Jian-Song , Ma Li , Gong Peng 
Published: June 2024

AbstractNeuroendocrine neoplasms (NENs) are highly heterogeneous and potentially malignant tumors arising from secretory cells of the neuroendocrine system. Gastroenteropancreatic neuroendocrine neoplasms (GEP-NENs) are the most common subtype of NENs. Historically, GEP-NENs have been regarded as infrequent and slow-growing malignancies; however, recent data have demonstrated that the worldwide prevalence and incidence of GEP-NENs have increased exponentially over the last three decades. In addition, an increasing number of studies have proven that GEP-NENs result in a limited life expectancy. These findings suggested that the natural biology of GEP-NENs is more aggressive than commonly assumed. Therefore, there is an urgent need for advanced researches focusing on the diagnosis and management of patients with GEP-NENs. In this review, we have summarized the limitations and recent advancements in our comprehension of the epidemiology, clinical presentations, pathology, molecular biology, diagnosis, and treatment of GEP-NETs to identify factors contributing to delays in diagnosis and timely treatment of these patients.
Native bacterial bioagents for management of potato soft rot disease caused by Pectobacterium carotovorum subsp. carotovorum
Authors: Almasoudi Najeeb M. , Al-Qurashi Adel D. , Elsayed Mohamed I. , Abo-Elyousr Kamal A. M. 
Published: June 2024

Abstract
Background
The reduced rate of potato production may occur under different conditions such as field, transit, storage, and marketing. Potato cultivation is frequently affected by various pathogens, among which Pectobacterium carotovorum subsp. carotovorum is a notorious bacterial pathogen responsible for causing bacterial soft rot disease. This pathogen poses a significant threat to potato production worldwide, resulting in substantial economic losses and food security concerns. This study aimed to investigate the effectiveness of three bacterial bioagents, namely Brevundimonas bullata, Bacillus siamensis, and Bacillus velezensis, against P. carotovorum subsp. carotovorum, a notorious bacterium responsible for causing potato tuber soft rot disease.

Results
Fifteen isolates were isolated from rhizosphere of potato plants. Out of 15 isolates, 3 isolates, No. 1, 11, and 12, showed highly antagonistic property to control the growth of P. carotovorum subsp. carotovorum in vitro. They were identified as B. bullata, B. velezensis, and B. siamensis, by using 16S rRNA nucleotide sequence analysis. Results from these experiments revealed that three bioagents exhibited notable inhibitory effects on the growth of the pathogenic bacterium. However, B. velezensis stood out as the most effective inhibitor among the tested bioagents, showcasing the highest level of bacteriostatic activity (2.6 cm). The findings from the in vitro experiments provided promising insights into the potential of these bacterial bioagents as natural antagonists against potato tuber soft rot disease. Building upon these promising results, the study recommended the utilization of all tested bioagents for controlling soft rot disease in potato tubers, especially during storage conditions where the risk of bacterial proliferation is heightened. Treatment with B. bullata, B. siamensis, and B. velezensis demonstrates varying degrees of efficacy in controlling the progression of soft rot disease. Notably, B. velezensis shows promising results with relatively low disease severity scores across all time points compared to the other treatments. B. velezensis demonstrates the lowest disease severity percentage at 7.00% compared to the other treatments.

Conclusions
The study underscores the significant potential of B. bullata, B. siamensis, and B. velezensis as effective biological control agents against potato tuber soft rot disease. By targeting the causative agent directly, these bioagents offer a promising avenue for mitigating the detrimental impact of soft rot disease on potato production, thereby contributing to improved crop yield and quality.