bluuebunny/crossref_metadata_2025_split huggingface dataset:
Asator, a tau‐tubulin kinase homolog inDrosophilalocalizes to the mitotic spindle
Authors: Qi Hongying , Yao Changfu , Cai Weili , Girton Jack , Johansen Kristen M. , Johansen Jørgen 
Published: November 2009

AbstractWe have used a yeast two‐hybrid interaction assay to identify Asator, a tau‐tubulin kinase homolog inDrosophilathat interacts directly with the spindle matrix protein Megator. Using immunocytochemical labeling by an Asator‐specific mAb as well as by transgenic expression of a GFP‐labeled Asator construct, we show that Asator is localized to the cytoplasm during interphase but redistributes to the spindle region during mitosis. Determination of transcript levels using qRT‐PCR suggested that Asator is expressed throughout development but at relatively low levels. By P‐element excision, we generated a null or strong hypomorphicAsatorexcallele that resulted in complete adult lethality when homozygous, indicating thatAsatoris an essential gene. That the observed lethality was caused by impaired Asator function was further supported by the partial restoration of viability by transgenic expression of Asator‐GFP in theAsatorexchomozygous mutant background. The finding that Asator localizes to the spindle region during mitosis and directly can interact with Megator suggests that its kinase activity may be involved in regulating microtubule dynamics and microtubule spindle function. Developmental Dynamics 238:3248–3256, 2009. © 2009 Wiley‐Liss, Inc.
Chondroitin sulfate expression is required for cardiac atrioventricular canal formation
Authors: Peal David S. , Burns C. Geoffrey , Macrae Calum A. , Milan David 
Published: November 2009

AbstractDefects in cardiac valvulogenesis are a common cause of congenital heart disease, and the study of this process promises to provide mechanistic insights and lead to novel therapeutics. Normal valve development involves multiple signaling pathways, and recently roles have been identified for extracellular matrix components, including glycosaminoglycans. We, therefore, explored the role of the glycosaminoglycan chondroitin sulfate during zebrafish cardiac development. Beginning at 33 hr, there is a distinct zone of chondroitin sulfate expression in the atrioventricular (AV) boundary, in the cardiac jelly between the endocardium and myocardium. This expression is both spatially and temporally restricted, and is undetectable after 48 hr. Chemical as well as genetic inhibition of chondroitin synthesis results in AV canal (AVC) defects, including loss of the atrioventricular constriction, blood regurgitation, and failure of circulation. Lack of chondroitin disrupts a marker of cell migration, results in a loss of myocardial and endothelial markers of valvulogenesis, and misregulates bone morphogenetic protein expression, supporting an early role in AVC development. In summary, we have defined a requirement for chondroitin sulfate expression in the normal patterning of the AV boundary, suggesting that this component of the cardiac jelly provides a necessary signal in this critical transition in vertebrate cardiogenesis. Developmental Dynamics 238:3103–3110, 2009. © 2009 Wiley‐Liss, Inc.
Muscleblind‐like 1 is a negative regulator of TGF‐β‐dependent epithelial–mesenchymal transition of atrioventricular canal endocardial cells
Authors: Vajda Natalie A. , Brimacombe Kyle R. , LeMasters Kathryn E. , Ladd Andrea N. 
Published: November 2009

AbstractThe development of the valves and septa of the heart depends on the formation and remodeling of endocardial cushions. Here, we report that the alternative splicing regulator muscleblind‐like 1 (MBNL1) exhibits a regionally restricted pattern of expression in canal region endocardium and ventricular myocardium during endocardial cushion development in chicken. Knockdown of MBNL1 in atrioventricular explants leads to a transforming growth factor β‐dependent increase in epithelial–mesenchymal transition (EMT) of endocardial cells. This reveals a novel role for MBNL1 during embryonic development, and represents the first evidence that an alternative splicing regulator is a key player in endocardial cushion development. Developmental Dynamics 238:3266–3272, 2009. © 2009 Wiley‐Liss, Inc.
Structural Consequences in α‐ and β‐Glucopyranosidato Complexes of Cp*TiCl3
Authors: Schwidom Dirk , Zeysing Daniela , Schmidt Michael , Heck Jürgen 
Published: November 2009

AbstractThe reaction of methyl‐4,6‐O‐(naphthyl‐2′‐methylidene)‐α‐D‐glucopyranoside (α‐MeNGH2) with trichloridopentamethylcyclopentadienyltitanium [Cp*TiCl3] and triethylamine in toluene reveals the dinuclear complex [(Cp*TiCl)‐μ‐(α‐MeNG)]2 (2). Complex 2 was characterized by elemental analysis, 1H and 13C NMR spectroscopy, circular dichroism, as well as single‐crystal structure analysis. Compared to the complex with the β‐anomer of the ligand in [(Cp*TiCl)‐μ‐(β‐MeNG)]2 (1), it is obvious that the absolute configuration of the Ti atoms in 2 have changed. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Characteristic Structures and Photophysical Properties of Nine‐Coordinate Europium(III) Complexes with Tandem‐Connected Tridentate Phosphane Oxide Ligands (Eur. J. Inorg. Chem. 32/2009)
Authors: Miyata Kohei , Hasegawa Yasuchika , Kuramochi Yusuke , Nakagawa Tetsuya , Yokoo Toshiaki , Kawai Tsuyoshi 
Published: November 2009

AbstractThe cover picture shows a red‐luminescent EuIII complex with a tandem‐connected tridentate phosphane oxide ligand. The coordination geometries of EuIII complexes with tridentate phosphane oxides provide characteristic distorted, capped square‐antiprism structures with nine coordinating oxygen atoms. EuIII complexes with tridentate phosphane oxides offer relatively high emission quantum yields as a result of their low‐symmetric and low‐vibrational frequency structures. The characteristic structures and photophysical properties of polyhedral f‐block metal complexes, nine‐coordinate EuIII complexes with tridentate phosphane oxides, are demonstrated for the first time. Details are discussed in the article by Y. Hasegawa, T. Kawai et al. on p. 4777 ff.
Catechol‐Based Macrocyclic Rods: En Route to Redox‐Active Molecular Switches
Authors: Weibel Nicolas , Mishchenko Artem , Wandlowski Thomas , Neuburger Markus , Leroux Yann , Mayor Marcel 
Published: November 2009

AbstractThe design and synthesis of the macrocyclic turnstile 1 comprising a terminally sulfur‐functionalized molecular rod and a redox‐active catechol subunit is described. The shape‐persistent macrocyclic scaffold consists of alternating arylene and ethynylene units. A freely rotating 2,6‐diethynyl‐catechol subunit is clamped between both terminal arylene subunits as molecular turnstile. While the electrochemical switching between the catechol and the quinone form of this catechol subunit is displayed by cyclic voltammetry, conformational rearrangements by favoring and disfavoring the formation of intramolecular hydrogen bonds are the subject of current investigations. Terminal acetyl‐protected sulfur anchor groups enabled the immobilization of the macrocycle between an Au tip and an Au substrate of a STM set‐up. Preliminary single‐molecule transport investigations of the turnstile 1 display comparable values as for the parent molecular rod. An electrochemically‐controlled single‐molecule transport experiment to investigate redox‐state‐dependent transport properties is currently under way.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
1,1′‐Binaphthyl‐2,2′‐diamine‐Based Chiral Phosphorous Triamides: Synthesis and Application in Asymmetric Catalysis
Authors: Barta Katalin , Eggenstein Matthias , Hölscher Markus , Franciò Giancarlo , Leitner Walter 
Published: November 2009

AbstractA set of chiral monodentate phosphorous triamides (PTA) comprising 1,1′‐binaphthyl‐2,2′‐diamine as the common moiety have been synthesised. Electronic and steric tuning of the ligands was achieved by variation of the substituents at the diamine nitrogen atoms by incorporating methyl, p‐tolyl and tosyl groups. Both chiral and achiral building blocks were used as monoamine components. Notably, (11bR)‐3,5‐dimethyl‐N,N‐bis[(S)‐1‐phenylethyl]‐3,5‐dihydro‐4H‐dinaphtho[2,1‐d:1′,2′‐f][1,3,2]diazaphosphepin‐4‐amine, which is the PTA most closely related to the Feringa phosphoramidite ligand, was synthesised and characterised by NMR spectroscopy and X‐ray diffraction. This PTA displays increased conformational rigidity in comparison with the corresponding phosphoramidite and adopts a C1‐symmetric structure both in the solid state and in solution, even at room temperature. The new PTA ligands were used in the copper‐catalysed conjugate addition of diethylzinc to cyclohex‐2‐enone and the nickel‐catalysed hydrovinylation of styrene giving good activities and chemoselectivities at moderate enantioselectivities in both reactions. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Discussion of ‘Seismic behavior of a single‐story asymmetric‐plan buildings under uniaxial excitation’ by A. Lucchini, G. Monti and S. Kunnath
Authors: Humar Jag Mohan , Fazileh Farrokh 
Published: November 2009

AbstractThis discussion examines the conclusion reached in the paper that in a single‐story asymmetric‐plan building the maximum displacement demand in the different resisting elements is reached for the same deformation configuration of the system and that the resultant of the seismic forces producing such demand is located at the center of resistance. It is shown that this conclusion is valid only for the particular model studied and cannot be generalized. Copyright © 2009 John Wiley & Sons, Ltd.
Complementary and alternative medicine use in patients presenting to a head and neck oncology clinic
Authors: Vyas Tarren , Hart Robert D. , Trites Jonathan R. , Philips Timothy J. , Archibald Kathleen E. M. , Phillips Judith E. , Taylor S. Mark 
Published: November 2009

AbstractBackgroundThe aim of this study was to determine the prevalence of complementary and alternative medicine (CAM) use among patients presenting to a head and neck oncology clinic prior to a diagnosis.MethodsThe study was conducted by administering questionnaires to 102 patients after being seen in the Head and Neck Oncology clinic for their initial consultation. The questionnaire assessed the extent of CAM use, types of CAMs used, and their reasons for use.ResultsA total of 132 CAMs were currently being used among 56 patients. The most common CAMs in use were multivitamins (26/132) and vitamin D (21/132). Meditation and yoga were associated with the greatest perceived benefit. The majority of patients obtained their information from family and friends. Most patients were using CAMs for physical health and well‐being.ConclusionAs CAM use among the population is widespread, it is important for clinicians to specifically address their use on initial presentation. © 2009 Wiley Periodicals, Inc. Head Neck, 2010
A spatially advancing turbulent flow and heat transfer in a curved channel
Authors: Matsubara Koji , Matsui Akihiko , Miura Takahiro , Sakurai Atsushi , Suto Hitoshi , Kawai Koji 
Published: November 2009

AbstractDirect numerical simulation was performed for a spatially advancing turbulent flow and heat transfer in a two‐dimensional curved channel, where one wall was heated to a constant temperature and the other wall was cooled to a different constant temperature. In the simulation, fully developed flow and temperature from the straight‐channel driver was passed through the inlet of the curved‐channel domain. The frictional Reynolds number was assigned 150, and the Prandtl number was given 0.71. Since the flow field was examined in the previous paper, the thermal features are mainly targeted in this paper. The turbulent heat flux showed trends consistent with a growing process of large‐scale vortices. In the curved part, the wall‐normal component of the turbulent heat flux was twice as large as the counterpart in the straight part, suggesting active heat transport of large‐scale vortices. In the inner side of the same section, temperature fluctuation was abnormally large compared with the modest fluctuation of the wall‐normal velocity. This was caused by the combined effect of the large‐scale motion of the vortices and the wide variation of the mean temperature; in such a temperature distribution, large‐scale ejection of the hot fluid near the outer wall, which is transported into the near inner‐wall region, should have a large impact on the thermal boundary layer near the inner wall. Wave number decomposition was conducted for various statistics, which showed that the contribution of the large‐scale vortex to the total turbulent heat flux normal to the wall reached roughly 80% inside the channel 135° downstream from the curved‐channel inlet. © 2009 Wiley Periodicals, Inc. Heat Trans Asian Res; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/htj.20275
Solidification of binary aqueous solution cooled from above
Authors: Kimura Shigeo , Nohara Yohei , Kiwata Takahiro , Komatsu Nobuyoshi 
Published: November 2009

AbstractFreezing and melting phenomena are important in many different fields, including crystal growth, casting, metallurgy, geophysics, and oceanography. Solidification of a multi‐component solution is the one often observed in nature. In order to investigate basic features of the freezing processes of binary systems, we conducted a series of laboratory experiments in a rectangular box cooled from above using aqueous NaNO3 solution. During the freezing, the solid phase always grows into many needle‐like crystals called the mushy layer. We measured the growth of the mushy layer thickness, the solid fraction, the temperature, and the concentration distributions. The average solid fraction is found to increase with time in the mushy layer. This causes a slow descent of the released solute in the mushy layer and its eventual fall into the liquid region below because of gravity. We propose a one‐dimensional model to explain the horizontally‐averaged mushy layer growth. In the model, the estimate of a heat flux at the mushy‐liquid interface due to natural convection is found essential for a correct prediction. The proposed theory predicts well the growth of the mushy‐layer and the average solid fraction, once the convective heat flux is properly given. © 2009 Wiley Periodicals, Inc. Heat Trans Asian Res; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/htj.20278
Effect of non‐uniformity of flow on velocity and turbulence intensities over a cobble‐bed
Authors: Afzalimehr Hossein 
Published: November 2009

AbstractNon‐uniform flows encompassing both accelerating and decelerating flows over a cobble‐bed flume have been experimentally investigated in a flume at a scale of intermediate relative submergence. Measurements of mean longitudinal flow velocity u, and determinations of turbulence intensities u′, v′, w′, and Reynolds shear stress −ufwf have been made. The longitudinal velocity distribution was divided into the inner zone close to the bed and the outer zone far from the bed. In the inner zone of the boundary layer (near the bed) the velocity profile closely followed the ‘Log Law’; however, in the outer zone the velocity distribution deviated from the Log Law consistently for both accelerating and decelerating flows and the changes in bed slopes ranging from −2% to + 2% had no considerable effect on the outer zone. For a constant bed slope (S = ±0·015), the larger the flow rate, the smaller the turbulence intensities. However, no detectable pattern has been observed for u′, v′ and w′ distributions near the bed. Likewise, for a constant flow rate (Q = 0·040 m3/s), with variation in bed slope the longitudinal turbulent intensity profile in the longitudinal direction remained concave for both accelerating and decelerating flows; whereas vertical turbulent intensity (w′) profile presented no specific form. The results reveal that the positions of maximum values of turbulence intensities and the Reynolds shear stress depend not only on the flow structure (accelerating or decelerating) but also on the intermediate relative submergence scale. Copyright © 2009 John Wiley & Sons, Ltd.
Reflective practice in infant mental health training and consultation
Authors: Larrieu Julie A. , Dickson Amy B. 
Published: November 2009

AbstractThis article describes models of training in infant mental health that utilize reflective supervision as a fundamental component of the educational and clinical experiences. The design and structure of these programs, offered by two medical centers, are described. Benefits and challenges to the adoption of infant mental health practice by trainees are outlined. Incorporation of reflective supervision in the training is discussed, and clinical examples are provided to illustrate its essential role in the development of the infant mental health clinician.
Strengthening and recognizing knowledge, skills, and reflective practice: The Michigan Association for Infant Mental Health competency guidelines and endorsement process
Authors: Weatherston Deborah J. , Kaplan‐Estrin Melissa , Goldberg Sheryl 
Published: November 2009

AbstractAttachment theory and research suggest that early intervention services focused on the infant–parent relationship can improve outcomes for the child (D. Cicchetti, F.A. Rogosch, & C.L. Toth, 2006; D. Cicchetti, C.L. Toth, & F.A. Rogosch, 1999; N.J. Cohen et al., 1999; C.M. Heinicke et al., 1999; K.T. Hoffman, R.S. Marvin, G. Cooper, & B. Powell, 2006; A.F. Lieberman, D. Weston, & J.H. Pawl, 1991; A.F. Lieberman & C.H. Zeanah, 1999; K. Lyons‐Ruth, D.B. Connell, & H.U. Grunebaum, 1990; P. Zeanah, B. Stafford, & C. Zeanah, 2005). For that reason, experts in the field of infant mental health have focused on the competencies needed for providing services to infants, young children, and their families (H.C. Quay, A.E. Hogan, & K.F. Donohue, 2009). While the multidisciplinary field of infant mental health has thrived over the last three decades, credentialing practitioners from such a wide range of disciplines presents considerable challenges (J. Korfmacher & A. Hilado, 2008). This article discusses those challenges in summarizing the development of a comprehensive set of competency guidelines and an accessible, effective procedure for professional endorsement in the infant and family field (D. Weatherston, B.D. Moss, & D. Harris, 2006). Criteria for endorsement encourage professionals from many disciplines to integrate new knowledge about infancy and early childhood mental health with strategies that are culturally sensitive and skillful. Emphasis on reflective supervision or consultation encourages a framework for best practice promoting professional growth (L. Eggbeer, T.L. Mann, & N. Seibel, 2007; J. Pawl, 1995). The authors report individual outcomes that support the specialization of infant mental health, as well as notable changes in educational and training programs and state policies promoting infant mental health.
Modulation of TRPM6 and Na+/Mg2+ exchange in mammary epithelial cells in response to variations of magnesium availability
Authors: Wolf Federica I. , Trapani Valentina , Simonacci Matteo , Mastrototaro Lucia , Cittadini Achille , Schweigel Monika 
Published: November 2009

AbstractMammary epithelial cells (HC11) chronically adapted to grow in a low‐magnesium (0.05 mM vs. 0.5 mM) or in a high‐magnesium (40 mM) medium were used to investigate on the mechanisms of cell magnesium transport under conditions of non‐physiological magnesium availability. Magnesium influx was higher in low‐magnesium cells compared to control or high‐magnesium cells, whereas magnesium efflux was higher in high‐magnesium cells compared to control and low‐magnesium cells. Magnesium efflux was partially inhibited by imipramine, inhibitor of the Na+/Mg2+ exchange. Using a monoclonal antibody detecting a ∼70 kDa protein associated with Na+/Mg2+ exchange activity, we found that the expression levels of this protein were proportional to magnesium efflux capacity, that is, high‐magnesium cells > control cells > low‐magnesium cells. As for magnesium influx, this was abolished by Co(III)hexaammine, inhibitor of magnesium channels. Surprisingly, we found that cells grown in low magnesium upregulated mRNA for the magnesium channel TRPM6, but not for other channels like TRPM7 or MagT1. TRPM6 mRNA was also rapidly upregulated or downregulated in HC11 cells deprived of magnesium or in low‐magnesium cells re‐added with magnesium, respectively. TRPM6 protein levels, as assessed by Western blot and immunofluorescence, underwent similar changes under comparable conditions. We propose that mammary epithelial cells adapt to decreased magnesium availability by upregulating magnesium influx via TRPM6, and counteract increased magnesium availability by increasing magnesium efflux primarily via Na+/Mg2+ exchange. These results show, for the first time, that TRPM6 contributes to regulating magnesium influx in mammary epithelial cells, similar to what is known for intestine and kidney. J. Cell. Physiol. 222: 374–381, 2010. © 2009 Wiley‐Liss, Inc.
Population doublings and percentage of S100‐positive cells as predictors of in vitro chondrogenicity of expanded human articular chondrocytes
Authors: Giovannini Samoa , Diaz‐Romero Jose , Aigner Thomas , Mainil‐Varlet Pierre , Nesic Dobrila 
Published: November 2009

AbstractThe aim of this study was to investigate the interconnection between the processes of proliferation, dedifferentiation, and intrinsic redifferentiation (chondrogenic) capacities of human articular chondrocyte (HAC), and to identify markers linking HAC dedifferentiation status with their chondrogenic potential. Cumulative population doublings (PD) of HAC expanded in monolayer culture were determined, and a threshold range of 3.57–4.19 PD was identified as indicative of HAC loss of intrinsic chondrogenic capacity in pellets incubated without added chondrogenic factors. While several specific gene and surface markers defined early HAC dedifferentiation process, no clear correlation with the loss of intrinsic chondrogenic potential could be established. CD90 expression during HAC monolayer culture revealed two subpopulations, with sorted CD90‐negative cells showing lower proliferative capacity and higher chondrogenic potential compared to CD90‐positive cells. Although these data further validated PD as critical for in vitro chondrogenesis, due to the early shift in expression, CD90 could not be considered for predicting chondrogenic potential of HAC expanded for several weeks. In contrast, an excellent mathematically modeled correlation was established between PD and the decline of HAC expressing the intracellular marker S100, providing a direct link between the number of cell divisions and dedifferentiation/loss of intrinsic chondrogenic capacity. Based on the dynamics of S100‐positive HAC during expansion, we propose asymmetric cell division as a potential mechanism of HAC dedifferentiation, and S100 as a marker to assess chondrogenicity of HAC during expansion, of potential value for cell‐based cartilage repair treatments. J. Cell. Physiol. 222: 411–420, 2010. © 2009 Wiley‐Liss, Inc.