bluuebunny/crossref_metadata_2025_split huggingface dataset:
Water use in a sugarcane plantation
Authors: Cabral Osvaldo M. R. , Rocha Humberto R. , Gash John H. , Ligo Marcos A. V. , Tatsch Jonatan D. , Freitas Helber C. , Brasilio Emília 
Published: January 2012

AbstractThe evapotranspiration (E) from a sugarcane plantation in the southeast Brazil was measured by the eddy‐covariance method during two consecutive cycles. These represented the second (393 days) and third year (374 days) re‐growth (ratoon). The total E in the first cycle was 829 mm, accounting for 69% of rainfall, whereas in the second cycle, it was 690 mm, despite the total rainfall (1353 mm) being 13% greater. The ratio of E to available energy, the evaporative fraction, exhibited a smaller variation between the first and second cycles: 0.58 and 0.51, respectively. The estimated interception losses were 88 and 90 mm, respectively, accounting for approximately 7% of the total rainfall. The sugarcane yield in the second cycle (61.5 ± 4.0 t ha−1) was 26% lower than the first cycle, as well as lower than the regional average for the third ratoon (76 t ha−1). The below average yield was associated with less available soil water at the beginning of the cycle, with the amount of rainfall recorded during the first 120 days of re‐growth in the second cycle being 16% of that recorded in the first (203 mm).
The Bunburra Rockhole meteorite fall in SW Australia: fireball trajectory, luminosity, dynamics, orbit, and impact position from photographic and photoelectric records
Authors: SPURNÝ Pavel , BLAND Philip A. , SHRBENÝ Lukáš , BOROVIČKA Jiří , CEPLECHA Zdeněk , SINGELTON Andrew , BEVAN Alex W. R. , VAUGHAN David , TOWNER Martin C. , McCLAFFERTY Terence P. , TOUMI Ralf , DEACON Geoff 
Published: January 2012

Abstract– We report an analysis of the first instrumentally observed meteorite fall in Australia, which was recorded photographically and photoelectrically by two eastern stations of the Desert Fireball Network (DFN) on July 20, 2007. The meteoroid with an initial mass of 22 kg entered the atmosphere with a low speed of 13.36 km s−1 and began a luminous trajectory at an altitude of 62.83 km. In maximum, it reached −9.6 absolute magnitude and terminated after a 5.7 s and 64.7 km long flight at an altitude of 29.59 km with a speed of 5.8 km s−1. The angle of the atmospheric trajectory to the Earth’s surface was 30.9°. The first organized search took place in October 2008 and the first meteorite (150 g) was found 97 m southward from the predicted central line at the end of the first day of searching (October 3, 2008). The second stone (174 g) was recovered 39 m northward from the central line, both exactly in the predicted mass limits. During the second expedition in February 2009, a third fragment of 14.9 g was found again very close (∼100 m) from the predicted position. Total recovered mass is 339 g. The meteorite was designated Bunburra Rockhole (BR) after a nearby landscape structure. This first DFN sample is an igneous achondrite. Initial petrography indicated that BR was a brecciated eucrite but detailed analyses proved that BR is not a typical eucrite, but an anomalous basaltic meteorite (Bland et al. 2009). BR was delivered from an unusual, Aten type orbit (a < 1 AU) where virtually the entire orbit was contained within Earth’s orbit. BR is the first achondrite fall with a known orbit and it is one of the most precise orbits ever calculated for a meteorite dropping fireball.
Changing Trends in Antimicrobial Resistance and Serotypes of Streptococcus pneumoniae Isolates in Asian Countries: an Asian Network for Surveillance of Resistant Pathogens (ANSORP) Study
Authors: Kim So Hyun , Song Jae-Hoon , Chung Doo Ryeon , Thamlikitkul Visanu , Yang Yonghong , Wang Hui , Lu Min , So Thomas Man-kit , Hsueh Po-Ren , Yasin Rohani M. , Carlos Celia C. , Pham Hung Van , Lalitha M. K. , Shimono Nobuyuki , Perera Jennifer , Shibl Atef M. , Baek Jin Yang , Kang Cheol-In , Ko Kwan Soo , Peck Kyong Ran 
Published: January 2012

ABSTRACT

Antimicrobial resistance in
Streptococcus pneumoniae
remains a serious concern worldwide, particularly in Asian countries, despite the introduction of heptavalent pneumococcal conjugate vaccine (PCV7). The Asian Network for Surveillance of Resistant Pathogens (ANSORP) performed a prospective surveillance study of 2,184
S. pneumoniae
isolates collected from patients with pneumococcal infections from 60 hospitals in 11 Asian countries from 2008 to 2009. Among nonmeningeal isolates, the prevalence rate of penicillin-nonsusceptible pneumococci (MIC, ≥4 μg/ml) was 4.6% and penicillin resistance (MIC, ≥8 μg/ml) was extremely rare (0.7%). Resistance to erythromycin was very prevalent in the region (72.7%); the highest rates were in China (96.4%), Taiwan (84.9%), and Vietnam (80.7%). Multidrug resistance (MDR) was observed in 59.3% of isolates from Asian countries. Major serotypes were 19F (23.5%), 23F (10.0%), 19A (8.2%), 14 (7.3%), and 6B (7.3%). Overall, 52.5% of isolates showed PCV7 serotypes, ranging from 16.1% in Philippines to 75.1% in Vietnam. Serotypes 19A (8.2%), 3 (6.2%), and 6A (4.2%) were the most prominent non-PCV7 serotypes in the Asian region. Among isolates with serotype 19A, 86.0% and 79.8% showed erythromycin resistance and MDR, respectively. The most remarkable findings about the epidemiology of
S. pneumoniae
in Asian countries after the introduction of PCV7 were the high prevalence of macrolide resistance and MDR and distinctive increases in serotype 19A.
Myxobacterium-Produced Antibiotic TA (Myxovirescin) Inhibits Type II Signal Peptidase
Authors: Xiao Yao , Gerth Klaus , Müller Rolf , Wall Daniel 
Published: January 2012

ABSTRACT

Antibiotic TA is a macrocyclic secondary metabolite produced by myxobacteria that has broad-spectrum bactericidal activity. The structure of TA is unique, and its molecular target is unknown. Here, we sought to elucidate TA's mode of action (MOA) through two parallel genetic approaches. First, chromosomal
Escherichia coli
TA-resistant mutants were isolated. One mutant that showed specific resistance toward TA was mapped and resulted from an IS
4
insertion in the
lpp
gene, which encodes an abundant outer membrane (Braun's) lipoprotein. In a second approach, the comprehensive
E. coli
ASKA plasmid library was screened for overexpressing clones that conferred TA
r
. This effort resulted in the isolation of the
lspA
gene, which encodes the type II signal peptidase that cleaves signal sequences from prolipoproteins. In whole cells, TA was shown to inhibit Lpp prolipoprotein processing, similar to the known LspA inhibitor globomycin. Based on genetic evidence and prior globomycin studies, a block in Lpp expression or prevention of Lpp covalent cell wall attachment confers TA
r
by alleviating a toxic buildup of mislocalized pro-Lpp. Taken together, these data argue that LspA is the molecular target of TA. Strikingly, the giant
ta
biosynthetic gene cluster encodes two
lspA
paralogs that we hypothesize play a role in producer strain resistance.
Identification of New Delhi metallo-β-lactamase gene (NDM-1) from a clinical isolate of Acinetobacter junii in China
Authors: Zhou Zhenwen , Guan Ruili , Yang Yiyu , Chen Ling , Fu Jie , Deng Qiulian , Xie Yongqiang , Huang Yong , Wang Jielin , Wang Daowen , Liao Can , Gong Sitang , Xia Huimin 
Published: January 2012

New Delhi metallo-β-lactamase-1 (NDM-1) is a novel type of metallo-β-lactamase (MBL) responsible for bacterial resistance to β-lactam antibiotics. Acinetobacter junii was previously shown to possess a MBL phenotype; however, the genes responsible for this phenotype were not identified. In this study, we reported the identification of NDM-1 gene in a clinical isolate of A. junii from a child patient in China, which was resistant to all β-lactams except aztreonam but sensitive to aminoglycosides and quinolones. The cloned NDM-1 gene contained an open reading frame of 813 bp and had a nucleotide sequence 99.9% identical (812/813) to reported NDM-1 genes carried by Acinetobacter baumannii , Enterococcus faecium , Escherichia coli , and Klebsiella pneumoniae . Recombinant NDM-1 protein was successfully expressed in E. coli BL21, and antibiotic sensitivities of the NDM-1-producing E. coli were largely similar to the A. junii 1454 isolate. The findings of this study raise attention to the emergence and spread of NDM-1-carrying bacteria in China.
Adoptive Cell Therapy for Lymphoma with CD4 T Cells Depleted of CD137-Expressing Regulatory T Cells
Authors: Goldstein Matthew J. , Kohrt Holbrook E. , Houot Roch , Varghese Bindu , Lin Jack T. , Swanson Erica , Levy Ronald 
Published: January 2012

Abstract
Adoptive immunotherapy with antitumor T cells is a promising novel approach for the treatment of cancer. However, T-cell therapy may be limited by the cotransfer of regulatory T cells (Treg). Here, we explored this hypothesis by using 2 cell surface markers, CD44 and CD137, to isolate antitumor CD4 T cells while excluding Tregs. In a murine model of B-cell lymphoma, only CD137negCD44hi CD4 T cells infiltrated tumor sites and provided protection. Conversely, the population of CD137posCD44hi CD4 T cells consisted primarily of activated Tregs. Notably, this CD137pos Treg population persisted following adoptive transfer and maintained expression of FoxP3 as well as CD137. Moreover, in vitro these CD137pos cells suppressed the proliferation of effector cells in a contact-dependent manner, and in vivo adding the CD137posCD44hi CD4 cells to CD137negCD44hi CD4 cells suppressed the antitumor immune response. Thus, CD137 expression on CD4 T cells defined a population of activated Tregs that greatly limited antitumor immune responses. Consistent with observations in the murine model, human lymphoma biopsies also contained a population of CD137pos CD4 T cells that were predominantly CD25posFoxP3pos Tregs. In conclusion, our findings identify 2 surface markers that can be used to facilitate the enrichment of antitumor CD4 T cells while depleting an inhibitory Treg population. Cancer Res; 72(5); 1239–47. ©2012 AACR.
Telomestatin Impairs Glioma Stem Cell Survival and Growth through the Disruption of Telomeric G-Quadruplex and Inhibition of the Proto-oncogene, c-Myb
Authors: Miyazaki Takeshi , Pan Yang , Joshi Kaushal , Purohit Deepti , Hu Bin , Demir Habibe , Mazumder Sarmistha , Okabe Sachiko , Yamori Takao , Viapiano Mariano , Shin-ya Kazuo , Seimiya Hiroyuki , Nakano Ichiro 
Published: January 2012

Abstract
Purpose: Glioma stem cells (GSC) are a critical therapeutic target of glioblastoma multiforme (GBM).
Experimental Design: The effects of a G-quadruplex ligand, telomestatin, were evaluated using patient-derived GSCs, non-stem tumor cells (non-GSC), and normal fetal neural precursors in vitro and in vivo. The molecular targets of telomestatin were determined by immunofluorescence in situ hybridization (iFISH) and cDNA microarray. The data were then validated by in vitro and in vivo functional assays, as well as by immunohistochemistry against 90 clinical samples.
Results: Telomestatin impaired the maintenance of GSC stem cell state by inducing apoptosis in vitro and in vivo. The migration potential of GSCs was also impaired by telomestatin treatment. In contrast, both normal neural precursors and non-GSCs were relatively resistant to telomestatin. Treatment of GSC-derived mouse intracranial tumors reduced tumor sizes in vivo without a noticeable cell death in normal brains. iFISH revealed both telomeric and non-telomeric DNA damage by telomestatin in GSCs but not in non-GSCs. cDNA microarray identified a proto-oncogene, c-Myb, as a novel molecular target of telomestatin in GSCs, and pharmacodynamic analysis in telomestatin-treated tumor-bearing mouse brains showed a reduction of c-Myb in tumors in vivo. Knockdown of c-Myb phenocopied telomestatin-treated GSCs both in vitro and in vivo, and restoring c-Myb by overexpression partially rescued the phenotype. Finally, c-Myb expression was markedly elevated in surgical specimens of GBMs compared with normal tissues.
Conclusions: These data indicate that telomestatin potently eradicates GSCs through telomere disruption and c-Myb inhibition, and this study suggests a novel GSC-directed therapeutic strategy for GBMs. Clin Cancer Res; 18(5); 1268–80. ©2012 AACR.
TRAIL Signaling and Synergy Mechanisms Used in TRAIL-Based Combination Therapies
Authors: Hellwig Christian T. , Rehm Markus 
Published: January 2012

Abstract
TRAIL and agonistic antibodies raised against TRAIL death receptors are highly promising new anticancer agents. In this brief review, we describe the recent advances in the molecular understanding of TRAIL signaling and the progress made in using TRAIL or agonistic antibodies clinically in mono- and combination therapies. Synergies have been reported in various scenarios of TRAIL-based multidrug treatments, and these can be used to potentiate the efficacy of therapies targeting TRAIL death receptors. We pay particular attention to structure the current knowledge on the diverse molecular mechanisms that are thought to give rise to these synergies and describe how different signaling features evoking synergies can be associated with distinct classes of drugs used in TRAIL-based combination treatments. Mol Cancer Ther; 11(1); 3–13. ©2012 AACR.
Quantitative Interactor Screening with next-generation Sequencing (QIS-Seq) identifies Arabidopsis thaliana MLO2 as a target of the Pseudomonas syringae type III effector HopZ2
Authors: Lewis Jennifer D , Wan Janet , Ford Rachel , Gong Yunchen , Fung Pauline , Nahal Hardeep , Wang Pauline W , Desveaux Darrell , Guttman David S 
Published: January 2012

AbstractBackgroundIdentification of protein-protein interactions is a fundamental aspect of understanding protein function. A commonly used method for identifying protein interactions is the yeast two-hybrid system.ResultsHere we describe the application of next-generation sequencing to yeast two-hybrid interaction screens and develop Quantitative Interactor Screen Sequencing (QIS-Seq). QIS-Seq provides a quantitative measurement of enrichment for each interactor relative to its frequency in the library as well as its general stickiness (non-specific binding). The QIS-Seq approach is scalable and can be used with any yeast two-hybrid screen and with any next-generation sequencing platform. The quantitative nature of QIS-Seq data make it amenable to statistical evaluation, and importantly, facilitates the standardization of experimental design, data collection, and data analysis. We applied QIS-Seq to identify theArabidopsis thalianaMLO2 protein as a target of thePseudomonas syringaetype III secreted effector protein HopZ2. We validate the interaction between HopZ2 and MLO2in plantaand show that the interaction is required for HopZ2-associated virulence.ConclusionsWe demonstrate that QIS-Seq is a high-throughput quantitative interactor screen and validate MLO2 as an interactor and novel virulence target of theP. syringaetype III secreted effector HopZ2.
"A novel in vivo model for the study of human breast cancer metastasis using primary breast tumor-initiating cells from patient biopsies"
Authors: Marsden Carolyn G , Wright Mary Jo , Carrier Latonya , Moroz Krzysztof , Pochampally Radhika , Rowan Brian G 
Published: January 2012

Abstract

Background
The study of breast cancer metastasis depends on the use of established breast cancer cell lines that do not accurately represent the heterogeneity and complexity of human breast tumors. A tumor model was developed using primary breast tumor-initiating cells isolated from patient core biopsies that would more accurately reflect human breast cancer metastasis.

Methods
Tumorspheres were isolated under serum-free culture conditions from core biopsies collected from five patients with clinical diagnosis of invasive ductal carcinoma (IDC). Isolated tumorspheres were transplanted into the mammary fat pad of NUDE mice to establish tumorigenicity in vivo. Tumors and metastatic lesions were analyzed by hematoxylin and eosin (H+E) staining and immunohistochemistry (IHC).

Results
Tumorspheres were successfully isolated from all patient core biopsies, independent of the estrogen receptor α (ERα)/progesterone receptor (PR)/Her2/neu status or tumor grade. Each tumorsphere was estimated to contain 50-100 cells. Transplantation of 50 tumorspheres (1-5 × 103 cells) in combination with Matrigel into the mammary fat pad of NUDE mice resulted in small, palpable tumors that were sustained up to 12 months post-injection. Tumors were serially transplanted three times by re-isolation of tumorspheres from the tumors and injection into the mammary fat pad of NUDE mice. At 3 months post-injection, micrometastases to the lung, liver, kidneys, brain and femur were detected by measuring content of human chromosome 17. Visible macrometastases were detected in the lung, liver and kidneys by 6 months post-injection. Primary tumors variably expressed cytokeratins, Her2/neu, cytoplasmic E-cadherin, nuclear β catenin and fibronectin but were negative for ERα and vimentin. In lung and liver metastases, variable redistribution of E-cadherin and β catenin to the membrane of tumor cells was observed. ERα was re-expressed in lung metastatic cells in two of five samples.

Conclusions
Tumorspheres isolated under defined culture conditions from patient core biopsies were tumorigenic when transplanted into the mammary fat pad of NUDE mice, and metastasized to multiple mouse organs. Micrometastases in mouse organs demonstrated a dormancy period prior to outgrowth of macrometastases. The development of macrometastases with organ-specific phenotypic distinctions provides a superior model for the investigation of organ-specific effects on metastatic cancer cell survival and growth.
Atorvastatin treatment is effective when used in combination with mefloquine in an experimental cerebral malaria murine model
Authors: Souraud Jean-Baptiste , Briolant Sébastien , Dormoi Jérome , Mosnier Joel , Savini Hélène , Baret Eric , Amalvict Rémy , Soulard Raoulin , Rogier Christophe , Pradines Bruno 
Published: January 2012

Abstract

Background
One of the major complications of Plasmodium falciparum infection is cerebral malaria (CM), which causes one million deaths worldwide each year, results in long-term neurological sequelae and the treatment for which is only partially effective. Statins are recognized to have an immunomodulatory action, attenuate sepsis and have a neuroprotective effect. Atorvastatin (AVA) has shown in vitro anti-malarial activity and has improved the activity of mefloquine (MQ) and quinine.

Methods
The efficiency of 40 mg/kg intraperitoneal AVA, alone or in association with MQ, was assessed in an experimental Plasmodium berghei ANKA rodent parasite model of CM and performed according to different therapeutic schemes. The effects on experimental CM were assessed through the evaluation of brain histopathological changes and neuronal apoptosis by TUNEL staining.

Results
AVA alone in the therapeutic scheme show no effect on survival, but the prophylactic scheme employing AVA associated with MQ, rather than MQ alone, led to a significant delay in mouse death and had an effect on the onset of CM symptoms and on the level of parasitaemia. Histopathological findings show a correlation between brain lesions and CM onset. A neuronal anti-apoptotic effect of AVA in the AVA + MQ combination was not shown.

Conclusions
The combination of AVA and MQ therapy led to a significant delay in mouse mortality. There were differences in the incidence, time to cerebral malaria and the level of parasitaemia when the drug combination was administered to mice. When used in combination with MQ, AVA had a relevant effect on the in vivo growth inhibition and clinical outcome of P. berghei ANKA-infected mice.
Both FA- and mPEG-conjugated chitosan nanoparticles for targeted cellular uptake and enhanced tumor tissue distribution
Authors: Hou Zhenqing , Zhan Chuanming , Jiang Qiwei , Hu Quan , Li Le , Chang Di , Yang Xiangrui , Wang Yixiao , Li Yang , Ye Shefang , Xie Liya , Yi Yunfeng , Zhang Qiqing 
Published: January 2012

Abstract
Both folic acid (FA)- and methoxypoly(ethylene glycol) (mPEG)-conjugated chitosan nanoparticles (NPs) had been designed for targeted and prolong anticancer drug delivery system. The chitosan NPs were prepared with combination of ionic gelation and chemical cross-linking method, followed by conjugation with both FA and mPEG, respectively. FA-mPEG-NPs were compared with either NPs or mPEG-/FA-NPs in terms of their size, targeting cellular efficiency and tumor tissue distribution. The specificity of the mPEG-FA-NPs targeting cancerous cells was demonstrated by comparative intracellular uptake of NPs and mPEG-/FA-NPs by human adenocarcinoma HeLa cells. Mitomycin C (MMC), as a model drug, was loaded to the mPEG-FA-NPs. Results show that the chitosan NPs presented a narrow-size distribution with an average diameter about 200 nm regardless of the type of functional group. In addition, MMC was easily loaded to the mPEG-FA-NPs with drug-loading content of 9.1%, and the drug releases were biphasic with an initial burst release, followed by a subsequent slower release. Laser confocal scanning imaging proved that both mPEG-FA-NPs and FA-NPs could greatly enhance uptake by HeLa cells. In vivo animal experiments, using a nude mice xenograft model, demonstrated that an increased amount of mPEG-FA-NPs or FA-NPs were accumulated in the tumor tissue relative to the mPEG-NPs or NPs alone. These results suggest that both FA- and mPEG-conjugated chitosan NPs are potentially prolonged drug delivery system for tumor cell-selective targeting treatments.
Hepatic reference gene selection in adult and juvenile female Atlantic salmon at normal and elevated temperatures
Authors: Anderson Kelli C , Elizur Abigail 
Published: January 2012

Abstract

Background
The use of quantitative real-time polymerase chain reaction (qPCR) has become widespread due to its specificity, sensitivity and apparent ease of use. However, experimental error can be introduced at many stages during sample processing and analysis, and for this reason qPCR data are often normalised to an internal reference gene. The present study used three freely available algorithms (GeNorm, NormFinder and BestKeeper) to assess the stability of hepatically expressed candidate reference genes (Hprt1, Tbp, Ef1α and β-tubulin) in two experiments. In the first, female Atlantic salmon (Salmo salar) broodstock of different ages were reared at either 14 or 22°C for an entire reproductive season, therefore a reference gene that does not respond to thermal challenge or reproductive condition was sought. In the second, estrogen treated juvenile salmon were maintained at the same temperatures for 14 days and a reference gene that does not respond to temperature or estrogen was required. Additionally, we performed independent statistic analysis to validate the outputs obtained from the program based analysis.

Results
Based on the independent statistical analysis performed the stability of the genes tested was Tbp > Ef1α > Hprt1 > β-tubulin for the temperature/reproductive development experiment and Ef1α > Hprt1 > Tbp for the estrogen administration experiment (β-tubulin was not analysed). Results from the algorithms tested were quite ambiguous for both experiments; however all programs consistently identified the least stable candidate gene. BestKeeper provided rankings that were consistent with the independent analysis for both experiments. When an inappropriate candidate reference gene was used to normalise the expression of a hepatically expressed target gene, the ability to detect treatment-dependent changes in target gene expression was lost for multiple groups in both experiments.

Conclusions
We have highlighted the need to independently validate the results of reference gene selection programs. In addition, we have provided a reference point for those wishing to study the effects of thermal challenge and/or hormonal treatment on gene stability in Atlantic salmon and other teleost species.