bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract SY29-03: Regulation and function of the Hippo-YAP pathway in organ size, tumorigenesis, and metastasis
Authors: Guan Kun-Liang Liang 
Published: August 2012

Abstract
Regulation and function of the Hippo-YAP pathway in organ size, tumorigenesis and metastasis Kun-Liang Guan, Department of Pharmacology and Moores Cancer Center, University of California at San Diego, USA The Yes-associated protein (YAP) is a transcription co-activator that plays a critical role in organ size control by promoting cell proliferation and inhibiting apoptosis. YAP is a human oncogene amplified or overexpressed in some human cancers. YAP represents the major downstream of the Hippo tumor suppressor pathway. The Lats tumor suppressor kinase in the Hippo pathway phosphorylates and inactivates YAP. Recent studies have demonstrated regulation of the Hippo pathway by cell-cell contact, cell adhesion, and mechanical stress. Cell attachment to extracellular matrix (ECM) is crucial to cell physiology such as cell polarity, cell motility, and cell proliferation. In normal cells, loss of attachment to ECM induces a specific type of apoptosis, termed anoikis. In metastatic cancer cells, resistance to anoikis allows their survival in circulation and dispersion to distant anatomic sites leading to tumor metastasis. We have observed that cell detachment activates the Hippo pathway kinases Lats1/2 and leads to YAP phosphorylation and inhibition. The detachment-induced YAP inactivation is required for anoikis. Furthermore, actin and microtubule organization mediates Lats1/2 activation in response to cell detachment. Moreover, Lats2 downregulation is tightly associated with metastatic prostate cancer, indicating a role of the Hippo pathway in tumor metastasis. Our findings provide an important connection from cell attachment and cytoskeleton organization to cell proliferation, anoikis, and cancer metastasis through the Hippo pathway.
Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr SY29-03. doi:1538-7445.AM2012-SY29-03
A Variational Approach for Retrieving Raindrop Size Distribution from Polarimetric Radar Measurements in the Presence of Attenuation
Authors: Cao Qing , Zhang Guifu , Xue Ming 
Published: August 2012

AbstractThis study presents a two-dimensional variational approach to retrieving raindrop size distributions (DSDs) from polarimetric radar data in the presence of attenuation. A two-parameter DSD model, the constrained-gamma model, is used to represent rain DSDs. Three polarimetric radar measurements—reflectivity ZH, differential reflectivity ZDR, and specific differential phase KDP—are optimally used to correct for the attenuation and retrieve DSDs by taking into account measurement error effects. Retrieval results with simulated data demonstrate that the proposed algorithm performs well. Applications to real data collected by the X-band Center for Collaborative Adaptive Sensing of the Atmosphere (CASA) radars and the C-band University of Oklahoma–Polarimetric Radar for Innovations in Meteorology and Engineering (OU-PRIME) also demonstrate the efficacy of this approach.
Documenting Wind Speed and Power Deficits behind a Utility-Scale Wind Turbine
Authors: Hirth Brian D. , Schroeder John L. 
Published: August 2012

AbstractHigh-spatial-and-temporal-resolution radial velocity measurements surrounding a single utility-scale wind turbine were collected using the Texas Tech University Ka-band mobile research radars. The measurements were synthesized to construct the first known dual-Doppler analyses of the mean structure and variability of a single turbine wake. The observations revealed a wake length that subjectively exceeded 20 rotor diameters, which far exceeds the typically employed turbine spacing of 7–10 rotor diameters. The mean horizontal wind speed deficits found within the turbine wake region relative to the free streamflow were related to potential reductions in the available power for a downwind turbine. Mean wind speed reductions of 17.4% (14.8%) were found at 7 (10) rotor diameters downwind, corresponding to a potential power output reduction of 43.6% (38.2%). The wind speed deficits found within the wake also exhibit large variability over short time intervals; this variability would have an appreciable impact on the inflow of a downstream turbine. The full understanding and application of these newly collected data have the potential to alter current wind-farm design and layout practices and to affect the cost of energy.
Constraints on the Profiles of Total Water PDF in AGCMs from AIRS and a High-Resolution Model
Authors: Molod Andrea 
Published: August 2012

Abstract
Atmospheric general circulation model (AGCM) cloud parameterizations generally include an assumption about the subgrid-scale probability distribution function (PDF) of total water and its vertical profile. In the present study, the Atmospheric Infrared Sounder (AIRS) monthly-mean cloud amount and relative humidity fields are used to compute a proxy for the second moment of an AGCM total water PDF called the “RH01 diagnostic,” which is the AIRS mean relative humidity for cloud fractions of 0.1 or less. The dependence of the second moment on horizontal grid resolution is analyzed using results from a high-resolution global model simulation.
The AIRS-derived RH01 diagnostic is generally larger near the surface than aloft, indicating a narrower PDF near the surface, and varies with the type of underlying surface. High-resolution model results show that the vertical structure of profiles of the AGCM PDF second moment is unchanged as the grid resolution changes from 200 to 100 to 50 km, and that the second-moment profiles shift toward higher values with decreasing grid spacing.
Several Goddard Earth Observing System, version 5 (GEOS-5), AGCM simulations were performed with several choices for the profile of the PDF second moment. The resulting cloud and relative humidity fields were shown to be quite sensitive to the prescribed profile, and the use of a profile based on the AIRS-derived proxy results in improvements relative to observational estimates. The AIRS-guided total water PDF profiles, including their dependence on underlying surface type and on horizontal resolution, have been implemented in the version of the GEOS-5 AGCM used for publicly released simulations.
High-Resolution Monthly Rainfall Database for Ethiopia: Homogenization, Reconstruction, and Gridding
Authors: Tsidu G. Mengistu 
Published: August 2012

Abstract
Recent heightened concern regarding possible consequences of anthropogenically induced global warming has spurred analyses of data aimed at detection of climate change and more thorough characterization of the natural climate variability. However, there is greater concern regarding the extent and especially quality of the historical climate data. In this paper, rainfall records of 233 gauge stations over Ethiopia for the 1978–2007 period are employed in an analysis that involves homogenization, reconstruction, and gridding onto a regular 0.5° × 0.5° resolution grid. Inhomogeneity is detected and adjusted based on quantile matching. The regularized expectation-maximization and multichannel singular spectrum analysis algorithms are then utilized for imputation of missing values, and the latter has been determined to have a marginal advantage. Ordinary kriging is used to create a gridded monthly rainfall dataset. The spatial and temporal coherence of this dataset are assessed using harmonic analysis, self-organizing maps, and intercomparison with global datasets. The self-organizing map delineates Ethiopia into nine homogeneous rainfall regimes, which is consistent with seasonal and interannual rainfall variations. The harmonic analysis of the dataset reveals that the annual mode accounts for 55%–85% of the seasonal rainfall variability over western Ethiopia while the semiannual mode accounts for up to 40% over southern Ethiopia. The dataset is also intercompared with Global Precipitation Climatology Project (GPCP), Climate Prediction Center (CPC) Merged Analysis of Precipitation (CMAP), Climatic Research Unit time series version 3 (CRUTS3.0), Tropical Rainfall Measuring Mission (TRMM), and interim ECMWF Re-Analysis (ERA-Interim) rainfall. The correlation of the dataset with global datasets ranges from 0.52 to 0.95 over sparse to dense rain gauge regions. The GPCP rainfall has a small bias and good correlation with the new dataset whereas TRMM and ERA-Interim have relatively large dry and wet biases, respectively.
The Winter Rainfall of Malaysia
Authors: Chen Tsing-Chang , Tsay Jenq-Dar , Yen Ming-Cheng , Matsumoto Jun 
Published: August 2012

Abstract
Malaysia is geographically separated into Peninsular Malaysia and west Borneo. The rainfall maximum in the former region occurs during November–December, whereas that in the latter region occurs during December–February. This difference of maximum rainfall period indicates that the formation mechanism is different for the rainfall centers in these two parts of Malaysia. Since rainfall is primarily produced by severe weather systems, the formation of a climatological rainfall center is explored through synoptic activity and the rainfall amount of this center is estimated through contributions by rain-producing disturbances. The major cause of the rainfall maximum of Peninsular Malaysia is cold surge vortices (CSVs) and heavy rainfall/flood (HRF) events propagating from the Philippine area and Borneo. In contrast, the major cause of the rainfall maximum of Borneo is these rain-producing disturbances trapped in Borneo. Disturbances of the former group are formed by the cold surge flows of the Philippine Sea type, whereas disturbances of the latter group are formed by cold surge flows of the South China Sea (SCS) type. The population of HRF events is about one-fourth of the rain-producing disturbances in both Peninsular Malaysia and Borneo, but they produce less than ~60% rainfall for these two regions. It is revealed from the synoptic and dynamic analyses that the major Borneo rain-producing disturbances propagate westward before December by strong tropical easterlies, but they are trapped after December by strong northeasterlies of the SCS-type cold surge flow.
Tornadoes in the New York Metropolitan Region: Climatology and Multiscale Analysis of Two Events
Authors: Colle Brian A. , Lombardo Kelly A. , Tongue Jeffrey S. , Goodman William , Vaz Nelson 
Published: August 2012

Abstract
This paper describes the climatology of tornadoes around New York City (NYC) and Long Island (LI), New York, and the structural evolution of two tornadic events that affected NYC on 8 August 2007 and 16 September 2010. Nearly half (18 of 34 events from 1950 to 2010) of NYC–LI tornadoes developed between 0500 and 1300 EDT, and August is the peak tornado month as compared to July for most of the northeast United States. A spatial composite highlights the approaching midlevel trough, moderate most unstable convective available potential energy (MUCAPE), and frontogenesis along a low-level baroclinic zone. Shortly before the early morning tornadoes on 8 August 2007, a mesoscale convective system intensified in the lee of the Appalachians in a region of low-level frontogenesis and moderate MUCAPE (~1500 J kg−1). Warm advection at low levels and evaporative cooling within an elevated mixed layer (EML) ahead of the mesoscale convective system (MCS) helped steepen the low-level lapse rates. Meanwhile, a surface mesolow along a quasi-stationary frontal zone enhanced the warm advection and low-level shear. The late afternoon event on 16 September 2010 was characterized by a quasi-linear convective system (QLCS) that also featured an EML aloft, a surface mesolow just west of NYC, low-level frontogenesis, and a southerly low-level jet ahead of an approaching midlevel trough. The QLCS intensified approaching NYC and generated mesovortices as the QLCS bowed outward. These cases illustrate the benefit of high-density surface observations, terminal Doppler radars, and sounding profiles from commercial aircraft for nowcasting these events.
ROCK1 functions as a critical regulator of stress erythropoiesis and survival by regulating p53
Authors: Vemula Sasidhar , Shi Jianjian , Mali Raghuveer Singh , Ma Peilin , Liu Yan , Hanneman Philip , Koehler Karl R. , Hashino Eri , Wei Lei , Kapur Reuben 
Published: August 2012

Abstract
Erythropoiesis is a dynamic, multistep process whereby hematopoietic stem cells differentiate toward a progressively committed erythroid lineage through intermediate progenitors. Although several downstream signaling molecules have been identified that regulate steady-state erythropoiesis, the major regulators under conditions of stress remain poorly defined. Rho kinases (ROCKs) belong to a family of serine/threonine kinases. Using gene-targeted ROCK1-deficient mice, we show that lack of ROCK1 in phenylhydrazine-induced oxidative stress model results in enhanced recovery from hemolytic anemia as well as enhanced splenic stress erythropoiesis compared with control mice. Deficiency of ROCK1 also results in enhanced survival, whereas wild-type mice die rapidly in response to stress. Enhanced survivability of ROCK1-deficient mice is associated with reduced level of reactive oxygen species. BM transplantation studies revealed that enhanced stress erythropoiesis in ROCK1-deficient mice is stem cell autonomous. We show that ROCK1 binds to p53 and regulates its stability and expression. In the absence of ROCK1, p53 phosphorylation and expression is significantly reduced. Our findings reveal that ROCK1 functions as a physiologic regulator of p53 under conditions of erythroid stress. These findings are expected to offer new perspectives on stress erythropoiesis and may provide a potential therapeutic target in human disease characterized by anemia.
The highly conserved 5' untranslated region as an effective target towards the inhibition of Enterovirus 71 replication by unmodified and appropriate 2'-modified siRNAs
Authors: Deng Jun-Xia , Nie Xiao-Jing , Lei Ying-Feng , Ma Chao-Feng , Xu Dong-Liang , Li Biao , Xu Zhi-Kai , Zhang Guo-Cheng 
Published: August 2012

Abstract

Background
Enterovirus 71 (EV71) is a highly infectious agent that plays an etiological role in hand, foot, and mouth disease. It is associated with severe neurological complications and has caused significant mortalities in recent large-scale outbreaks. Currently, no effective vaccine or specific clinical therapy is available against EV71.

Methods
Unmodified 21 nucleotide small interfering RNAs (siRNAs) and classic 2′-modified (2′-O-methylation or 2′-fluoro modification) siRNAs were designed to target highly conserved 5′ untranslated region (UTR) of the EV71 genome and employed as anti-EV71 agents. Real-time TaqMan RT-PCR, western blot analysis and plaque assays were carried out to evaluate specific viral inhibition by the siRNAs.

Results
Transfection of rhabdomyosarcoma (RD) cells with siRNAs targeting the EV71 genomic 5′ UTR significantly delayed and alleviated the cytopathic effects of EV71 infection, increased cell viability in EV71-infected RD cells. The inhibitory effect on EV71 replication was sequence-specific and dosage-dependent, with significant corresponding decreases in viral RNA, VP1 protein and viral titer. Appropriate 2′-modified siRNAs exhibited similar RNA interference (RNAi) activity with dramatically increased serum stability in comparison with unmodified counterparts.

Conclusion
Sequences were identified within the highly conserved 5′ UTR that can be targeted to effectively inhibit EV71 replication through RNAi strategies. Appropriate 2′-modified siRNAs provide a promising approach to optimizing siRNAs to overcome barriers on RNAi-based antiviral therapies for broader administration.
Immune responses in the lungs of patients with tuberculous pleural effusion without pulmonary tuberculosis
Authors: Qama Diana , Choi Won-Il , Kwon Kun Young 
Published: August 2012

AbstractBackgroundTuberculous pleural effusion (TPE) is one of the most common forms of extrapulmonary tuberculosis. Because most studies of TPE focused on the pleural space, little information regarding lung parenchyma is available. We therefore aimed to investigate immune responses in the lung parenchyma of TPE patients without pulmonary tuberculosis.MethodsPatients with any evidence of pulmonary tuberculosis, either from radiologic or bacteriologic evaluation, were excluded. Bronchoalveolar lavage fluid (BALF) was collected from 10 newly diagnosed, untreated, HIV-negative TPE patients and 10 healthy controls. We analyzed T-lymphocyte subpopulations and measured 10 cytokines in BALF. Cytokine levels in BALF were standardised using urea.ResultsThe concentrations of interferon-γ (IFN-γ), tumor necrosis factor-α (TNF-α), vascular endothelial growth factor (VEGF), and the CD4+/CD8+ ratio of T-lymphocytes were significantly higher in TPE patients without pulmonary tuberculosis than in the controls. Of the cytokines measured in BALF, VEGF showed the highest concentration. No difference was observed in T-helper type 2 cytokines between the 2 groups.ConclusionThere were significant immune responses and increases in IFN-γ, TNF-α, and VEGF in the lung parenchyma of TPE patients without pulmonary tuberculosis. This result suggests that TPE may induce a significant immune response in lung parenchyma.
Acid stress response and protein induction in Campylobacter jejuni isolates with different acid tolerance
Authors: Birk Tina , Wik Monica Takamiya , Lametsch René , Knøchel Susanne 
Published: August 2012

Abstract

Background
During the transmission route from poultry to the human host, the major foodborne pathogen C. jejuni may experience many types of stresses, including low pH caused by different acids. However, not all strains are equally sensitive to the stresses. The aim of this study was to investigate the response to acid stress of three sequenced C. jejuni strains with different acid tolerances using HCl and acetic acid.

Results
Two-dimensional gel electrophoresis was used for proteomic analysis and proteins were radioactively labelled with methionine to identify proteins only related to acid exposure. To allow added radioactive methionine to be incorporated into induced proteins, a modified chemically defined broth was developed with the minimal amount of methionine necessary for satisfactory growth of all strains. Protein spots were analyzed using image software and identification was done with MALDI-TOF-TOF. The most acid-sensitive isolate was C. jejuni 327, followed by NCTC 11168 and isolate 305 as the most tolerant. Overall, induction of five proteins was observed within the pI range investigated: 19 kDa periplasmic protein (p19), thioredoxin-disulfide (TrxB), a hypothetical protein Cj0706 (Cj0706), molybdenum cofactor biosynthesis protein (MogA), and bacterioferritin (Dps). Strain and acid type dependent differences in the level of response were observed. For strain NCTC 11168, the induced proteins and the regulator fur were analysed at the transcriptomic level using qRT-PCR. In this transcriptomic analysis, only up-regulation of trxB and p19 was observed.

Conclusions
A defined medium that supports the growth of a range of Campylobacter strains and suitable for proteomic analysis was developed. Mainly proteins normally involved in iron control and oxidative stress defence were induced during acid stress of C. jejuni. Both strain and acid type affected sensitivity and response.
The effectiveness of a chair intervention in the workplace to reduce musculoskeletal symptoms. A systematic review
Authors: van Niekerk Sjan-Mari , Louw Quinette Abigail , Hillier Susan 
Published: August 2012

Abstract

Background
Prolonged sitting has been associated with musculoskeletal dysfunction. For desk workers, workstation modifications frequently address the work surface and chair. Chairs which can prevent abnormal strain of the neuromuscular system may aid in preventing musculo-skeletal pain and discomfort. Anecdotally, adjustability of the seat height and the seat pan depth to match the anthropometrics of the user is the most commonly recommended intervention. Within the constraints of the current economic climate, employers demand evidence for the benefits attributed to an investment in altering workstations, however this evidence-base is currently unclear both in terms of the strength of the evidence and the nature of the chair features. The purpose of this study was to evaluate the evidence for the effectiveness of chair interventions in reducing workplace musculoskeletal symptoms.

Methods
Pubmed, Cinahl, Pedro, ProQuest, SCOPUS and PhysioFocus were searched. ‘Ergonomic intervention’, ‘chair’, ‘musculoskeletal symptoms’, ‘ergonomics’, ‘seated work’ were used in all the databases. Articles were included if they investigated the influence of chair modifications as an intervention; participants were in predominantly seated occupations; employed a pre/post design (with or without control or randomising) and if the outcome measure included neuro-musculoskeletal comfort and/or postural alignment. The risk of bias was assessed using a tool based on The Cochrane Handbook.

Results
Five studies were included in the review. The number of participants varied from 4 to 293 participants. Three of the five studies were Randomised Controlled Trials, one pre and post-test study was conducted and one single case, multiple baselines (ABAB) study was done. Three studies were conducted in a garment factory, one in an office environment and one with university students. All five studies found a reduction in self-reported musculoskeletal pain immediately after the intervention. Bias was introduced due to poor randomization procedures and lack of concealed allocation. Meta-analysis was not possible due to the heterogeneity of the data (differing population, intervention and outcomes across studies).

Conclusion
The findings of this review indicate a consistent trend that supports the role of a chair intervention to reduce musculoskeletal symptoms among workers who are required to sit for prolonged periods. However the amount, level and quality of the evidence are only moderate therefore we cannot make strong recommendations until further trials are conducted. The review also highlights gaps: for example in showing whether the effectiveness of a chair intervention has long-term impact, particularly with respect to musculoskeletal symptoms, as well as the recurrence of symptoms and the consequent cost of care.