bluuebunny/crossref_metadata_2025_split huggingface dataset:
Juvenile Scleredema of Buschke
Authors: Rani J Dhanuja , Patil Suneel G , Murthy ST Srinivas , Koshy Ajit V , Nagpal Deepak , Gupta Sheeba 
Published: May 2012

ABSTRACTAimTo recognize a line of treatment for scleredema of Buschke in an adolescent.BackgroundScleredema of Buschke is an uncommon disorder characterized by induration of the skin, which includes a non pitting hardening of the skin around the neck, shoulders, and trunk sometimes the face. Three variants are recognized. The histopathologic features of scleredema are characterized by thickened collagen bundles within the reticular dermis that are separated by mucin containing fenestrations. No consistent treatment modality is currently followed.Case reportThe present case report describes scleredema of Buschke in a 10-year-old female child reported with stiffness of facial skin and difficulty in opening the mouth. The patient was treated with antibiotics and vitamin supplements and there was drastic improvement with decrease in skin stiffness and increase in mouth opening. The stiffness of facial muscles decreased and mouth opening increased.ConclusionMultiple treatment modalities for scleredema have been used. In the present case, high-dose antibiotic therapy was used with good prognosis.Clinical significanceHigh-dose antibiotic therapy can be used for treatment of dermatologic disorders with reasonably good prognosis.How to cite this articleRani JD, Patil SG, Murthy STS, Koshy AV, Nagpal D, Gupta S. Juvenile Scleredema of Buschke. J Contemp Dent Pract 2012;13(1):111-114.
Management of Dens Invaginatus Type III with Large Periradicular Lesion
Authors: de Souza Falcão Luciana , de Freitas Priscila Sousa , de Oliveira Marreiro Raquel , Garrido Ângela Delfina Bittencourt 
Published: May 2012

ABSTRACT

Aim
This study describes a clinical case of type III dens invaginatus with an extensive periradicular lesion treated successfully.

Background
Dens invaginatus is a maldevelopment of the dental germ which occurs as a result of the invagination of the enamel organ. These cases may present difficulties with respect to its diagnosis and treatment because of canal morphology. The success of endodontic therapy requires a knowledge of dental anatomy and its anomalies.

Case report
A 17-year-old female patient is reported presenting right maxillary lateral incisor (tooth no. 7) classified as type III dens invaginatus with necrotic pulp and presence of an extensive radiolucid lesion. Endodontic treatment was recommended for tooth. However, intracanal exudate was present, suggesting a resistant infection. Enucleation of the lesion was performed as a complementary approach. The root canal obturation was carried out by the gutta-percha thermoplastification technique with root canal sealer, followed by restoration of the tooth. Healing of the lesion with hard tissue formation was confirmed at follow-up.

Conclusion
A combination of endodontic and surgical treatments were fundamental to the maintenance of the tooth. The treatment was considered successful.

Clinical significance
Root canal therapy of dens invaginatus should be based on a thorough clinical and radiographic evaluation. The knowledge of classification and anatomical variations of teeth with dens invaginatus are of great importance for correct treatment.

How to cite this article
de Souza Falcão L, de Freitas PS, de Oliveira Marreiro R, Garrido ÂDB. Management of Dens Invaginatus Type III with Large Periradicular Lesion. J Contemp Dent Pract 2012;13(1):119-124.
Fracture Strength of Three All-Ceramic Systems: Top-Ceram compared with IPS-Empress and In-Ceram
Authors: Quran Firas Al , Haj-Ali Reem 
Published: May 2012

ABSTRACT

Purpose
The purpose of this study was to investigate the fracture loads and mode of failure of all-ceramic crowns fabricated using Top-Ceram and compare it with all-ceramic crowns fabricated from well-established systems: IPS-Empress II, In-Ceram.

Materials and methods
Thirty all-ceramic crowns were fabricated; 10 IPS-Empress II, 10 In-Ceram alumina and 10 Top-Ceram. Instron testing machine was used to measure the loads required to introduce fracture of each crown.

Results
Mean fracture load for In-Ceram alumina [941.8 (± 221.66) N] was significantly (p < 0.05) higher than those of Top-Ceram and IPS-Empress II. There was no statistically significant difference between Top-Ceram and IPS-Empress II mean fracture loads; 696.20 (+222.20) and 534 (+110.84) N respectively. Core fracture pattern was highest seen in Top- Ceram specimens.

How to cite this article
Quran AF, Haj-Ali R. Fracture Strength of Three All-Ceramic Systems: Top-Ceram Compared with IPSEmpress and In-Ceram. J Contemp Dent Pract 2012;13(2): 210-215.
Peak Performance Training Using Prefrontal EEG Biofeedback
Authors: Sokhadze Estate “Tato” 
Published: May 2012

The use of biofeedback training to self-regulate EEG patterns with the aim of recovering or optimizing function and behavioral performance is becoming increasingly established. The most reasonable approach is to learn to generate and maintain optimal brain wave patterns and produce associated peak performance states on demand. We report two studies where 12 sessions of prefrontal EEG feedback were used to improve performance in both clinical and nonclinical populations. Neurofeedback using Focus, Alertness, and 40 Hz (Neureka!) measures resulted in improved selective attention and other cognitive functions. We discuss other potential applications of neurofeedback in the areas of “under-pressure” activity, where peak performance state is an essential part of the job, such as in sports or the performing arts, as well as for human operators, such as air traffic dispatchers and military personnel on duty.
Insertion of Permanent Hemodialysis Catheters through the Great Saphenous Vein
Authors: Skandalos Ioannis K. , Samaras Asterios A. , Karakatsanis Anestis I. , Ditsias Theodoros K. , Filippidis Antis A. , Mavromatidis Konstantinos S. , Koutsouflianiotis Nikolaos K. 
Published: May 2012

Introduction: In patients with end stage renal disease when there is inability for the creation of a direct arterio-venous fistula or by using a graft, the insertion of a permanent hemodialysis catheter in a central vein ensures the conduction of hemodialysis. We present a technique of placing a permanent catheter in the inferior vena cava through the great saphenous vein without the use of a guidewire. Patients and Methods: Over the study period, 12 hemodialyzed patients, with a mean age of 73 years, were referred for placement of a dialysis catheter through the great saphenous veins. The procedure was performed under local anesthesia, ECG monitoring, and fluoroscopic control. The insertion of the tunneled hemodialysis catheters was accomplished with ease, through surgical exposure of the great saphenous vein, without the need for a guidewire. Results: The hemodialysis catheter's function was assessed intraoperatively No intraoperative or immediate post operative complications were noted while during the study period 3 thromboses and an infection were detected (0,95 per 1000 catheter days). The primary catheter patency rates were 92%, 84%, 54% at 30, 90 and 180 days respectively, varying from 28 to 845 days (mean±SD = 294 ± 243,3). Conclusion: The introduction of dialysis catheters in the inferior vena cava through the great saphenous vein is technically simple with rare complications and with higher patency rates compared to the traditional femoral approach.
The effect of a visual aid on the comprehension of cataract surgery in a rural, indigent South Indian population
Authors: Karan Abraar M. , Campbell Daniel J. , Mayer Hylton R. 
Published: May 2012

PurposeTo determine whether a visual aid improves the understanding and retention of information presented during informed consent for rural, indigent patients presenting for cataract surgery.
Subjects and MethodsThis was a randomized, unmasked, interventional study. We recruited patients who presented to the Hande Surgical Hospital in Chennai, India, for cataract surgery. Patients were randomized into two groups: verbal consent alone (group A) and verbal consent plus a poster (group B). Both groups completed an 11-question true/false quiz immediately before and after informed consent and one day after surgery.
ResultsA total of 60 patients were recruited for the study, with 30 randomly assigned to each group; 23 patients from group A and 17 from group B completed the study. Informed consent improved patient scores in both groups; however, group B had significantly higher mean scores on postoperative day 1 (7.4 vs 8.7, P = 0.005) and significantly greater improvement in mean scores from pre-informed consent to postoperative day 1 (1.3 vs 3.6, P = 0.002).
ConclusionInformed consent improves patient understanding of cataract surgery. Using a visual aid during informed consent for cataract surgery improves understanding and retention of information more than verbal consent alone in a rural South Indian population.
Building Community Resilience: What Can the United States Learn From Experiences in Other Countries?
Authors: Moore Melinda , Chandra Anita , Feeney Kevin C. 
Published: May 2012

AbstractObjectivesCommunity resilience (CR) is emerging as a major public policy priority within disaster management and is one of two key pillars of the December 2009 US National Health Security Strategy. However, there is no clear agreement on what key elements constitute CR. We examined exemplary practices from international disaster management to validate the elements of CR, as suggested by Homeland Security Presidential Directive 21 (HSPD-21), to potentially identify new elements and to identify practices that could be emulated or adapted to help build CR.MethodsWe extracted detailed information relevant to CR from unpublished case studies we had developed previously, describing exemplary practices from international natural disasters occurring between 1985 and 2005. We then mapped specific practices against the five elements of CR suggested by HSPD-21.ResultsWe identified 49 relevant exemplary practices from 11 natural disasters in 10 countries (earthquakes in Mexico, India, and Iran; volcanic eruption in Philippines; hurricanes in Honduras and Cuba; floods in Bangladesh, Vietnam, and Mozambique; tsunami in Indian Ocean countries; and typhoon in Vietnam). Of these, 35 mapped well against the five elements of CR: community education, community empowerment, practice, social networks, and familiarity with local services; 15 additional practices were related to physical security and economic security. The five HSPD-21 CR elements and two additional ones we identified were closely related to one another; social networks were especially important to CR.ConclusionsWhile each disaster is unique, the elements of CR appear to be broadly applicable across countries and disaster settings. Our descriptive study provides retrospective empirical evidence that helps validate, and adds to, the elements of CR suggested by HSPD-21. It also generates hypotheses about factors contributing to CR that can be tested in future analytic or experimental research. (Disaster Med Public Health Preparedness. 2013;7:292-301)
Inhibition of caspase‐9 by stabilized peptides targeting the dimerization interface
Authors: Huber Kristen L. , Ghosh Sumana , Hardy Jeanne A. 
Published: May 2012

AbstractCaspases comprise a family of dimeric cysteine proteases that control apoptotic programmed cell death and are therefore critical in both organismal development and disease. Specific inhibition of individual caspases has been repeatedly attempted, but has not yet been attained. Caspase‐9 is an upstream or initiator caspase that is regulated differently from all other caspases, as interaction with natural inhibitor X‐linked inhibitor of apoptosis protein (XIAP)‐baculovirus inhibitory repeat 3 (BIR3) occurs at the dimer interface maintaining caspase‐9 in an inactive monomeric state. One route to caspase‐9‐specific inhibition is to mimic this interaction, which has been localized to the α5 helix of XIAP‐BIR3. We have developed three types of stabilized peptides derived from the α5 helix, using incorporation of aminoisobutyric acid, the avian pancreatic polypeptide (aPP)‐scaffold or aliphatic staples. The stabilized peptides are helical in solution and achieve up to 32 μM inhibition, indicating that this allosteric site at the caspase‐9 dimerization interface is regulatable with low‐molecular weight synthetic ligands and is thus a druggable site. The most potent peptides against caspase‐9 activity are the aPP‐scaffolded peptides. Other caspases, which are not regulated by dimerization, should not be inactivated by these peptides. Given that all of the peptides attain helical structures but cannot recapitulate the high‐affinity inhibition of the intact BIR3 domain, it has become clear that interactions of caspase‐9 with the BIR3 exosite are essential for high‐affinity binding. These results explain why the full XIAP‐BIR3 domain is required for maximal inhibition and suggest a path forward for achieving allosteric inhibition at the dimerization interface using peptides or small molecules. © 2012 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 98: 451–465, 2012.
A novel hydroxyproline rich glycopeptide from pericarp of Datura stramonium: Proficiently eradicate the biofilm of antifungals resistant Candida albicans
Authors: Mandal Santi M. 
Published: May 2012

AbstractThe increasing threats of multidrug resistant fungal pathogens, several studies have been focused to identify novel antifungal plant peptides with unique characteristics. Plants have been defending themselves against fungal infection by generating effective antifungal molecules. Here, a novel antifungal peptide with molecular mass of 4.0 kDa was purified from the pericarp of D.stramonium using reversed phase chromatography system after acetic acid extraction. Presence of sugar moieties (‐GlcNAc‐) in the peptide have been confirmed using thin layer chromatographic (TLC), CD polarimeter, and MALDI MS analysis. Complete amino acid sequences of this peptide by PSD MALDI MS analysis revealed to contain hydroxyproline in the centre of two cysteine residues. After sequencing “TFPKCAPTRhyPhy PGPKhyPCDINNFKSKFWHIWRA‐(GlcNAc‐)Asn” peptide named as “datucin.” Antifungal sensitivity of datucin have been performed for both planktonic cells and biofilm phenotype of a multidrug resistant clinical isolates, C. albicans and showed a MIC and MBEC values of 1 μM and 2 μM, respectively. Hence, D. stramonium offers a potential source of novel antifungal peptide datucin with possible utility in antifungal chemotherapy. © 2012 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 98: 332–337, 2012.
pH, redox potential and local biofilm potential microenvironments within Geobacter sulfurreducens biofilms and their roles in electron transfer
Authors: Babauta Jerome T. , Nguyen Hung Duc , Harrington Timothy D. , Renslow Ryan , Beyenal Haluk 
Published: May 2012

AbstractThe limitation of pH inside electrode‐respiring biofilms is a well‐known concept. However, little is known about how pH and redox potential are affected by increasing current inside biofilms respiring on electrodes. Quantifying the variations in pH and redox potential with increasing current is needed to determine how electron transfer is tied to proton transfer within the biofilm. In this research, we quantified pH and redox potential variations in electrode‐respiring Geobacter sulfurreducens biofilms as a function of respiration rates, measured as current. We also characterized pH and redox potential at the counter electrode. We concluded that (1) pH continued to decrease in the biofilm through different growth phases, showing that the pH is not always a limiting factor in a biofilm and (2) decreasing pH and increasing redox potential at the biofilm electrode were associated only with the biofilm, demonstrating that G. sulfurreducens biofilms respire in a unique internal environment. Redox potential inside the biofilm was also compared to the local biofilm potential measured by a graphite microelectrode, where the tip of the microelectrode was allowed to acclimatize inside the biofilm. Biotechnol. Bioeng. 2012; 109: 2651–2662. © 2012 Wiley Periodicals, Inc.
Caprylic acid precipitation method for impurity reduction: An alternative to conventional chromatography for monoclonal antibody purification
Authors: Brodsky Yan , Zhang Cheng , Yigzaw Yinges , Vedantham Ganesh 
Published: May 2012

AbstractWe report the use of caprylic acid based impurity precipitation as (1) an alternative method to polishing chromatography techniques commonly used for monoclonal antibody purification and (2) an impurity reduction step prior to harvesting the bioreactor. This impurity reduction method was tested with protein A purified antibodies and with cell culture fluid. First, the operational parameters influencing precipitation of host cell proteins and high molecular weight aggregate in protein A pools were investigated. When used as a polishing step, the primary factor affecting purification and yield was determined to be pH. Caprylic acid precipitation was comparable to polishing IEX chromatography in reducing host cell protein and aggregate levels. A virus reduction study showed complete clearance of a model retrovirus during caprylic acid precipitation of protein A purified antibody. Caprylic acid mediated impurity precipitation in cell culture showed that the impurity clearance was generally insensitive to pH and caprylic acid concentration whereas yield was a function of caprylic acid concentration. Protein A purification of caprylic acid precipitated cell culture fluid generated less turbid product pool with reduced levels of host cell proteins and high molecular weight aggregate. The results of this study show caprylic acid precipitation to be an effective purification method that can be incorporated into a production facility with minimal cost as it utilizes existing tanks and process flow. Eliminating flow through chromatography polishing step can provide process intensification by avoiding the process tank volume constraints for high titer processes. Biotechnol. Bioeng. 2012; 109: 2589–2598. © 2012 Wiley Periodicals, Inc.
The demonstration of a novel sulfur cycle‐based wastewater treatment process: Sulfate reduction, autotrophic denitrification, and nitrification integrated (SANI®) biological nitrogen removal process
Authors: Lu Hui , Wu Di , Jiang Feng , Ekama George A. , van Loosdrecht Mark C.M. , Chen Guang‐Hao 
Published: May 2012

AbstractSaline water supply has been successfully practiced for toilet flushing in Hong Kong since 1950s, which saves 22% of freshwater in Hong Kong. In order to extend the benefits of saline water supply into saline sewage management, we have recently developed a novel biological organics and nitrogen removal process: the Sulfate reduction, Autotrophic denitrification, and Nitrification Integrated (SANI®) process. The key features of this novel process include elimination of oxygen demand in organic matter removal and production of minimal sludge. Following the success of a 500‐day lab‐scale trial, this study reports a pilot scale evaluation of this novel process treating 10 m3/day of 6‐mm screened saline sewage in Hong Kong. The SANI® pilot plant consisted of a sulfate reduction up‐flow sludge bed (SRUSB) reactor, an anoxic bioreactor for autotrophic denitrification and an aerobic bioreactor for nitrification. The plant was operated at a steady state for 225 days, during which the average removal efficiencies of both chemical oxygen demand (COD) and total suspended solids (TSS) at 87% and no excess sludge was purposefully withdrawn. Furthermore, a tracer test revealed 5% short circuit flow and a 34.6% dead zone in the SRUSB, indicating a good possibility to further optimize the treatment capacity of the process for full‐scale application. Compared with conventional biological nitrogen removal processes, the SANI® process reduces 90% of waste sludge, which saves 35% of the energy and reduces 36% of fossil CO2 emission. The SANI® process not only eliminates the major odor sources originating from primary treatment and subsequent sludge treatment and disposal during secondary saline sewage treatment, but also promotes saline water supply as an economic and sustainable solution for water scarcity and sewage treatment in water‐scarce coastal areas. Biotechnol. Bioeng. 2012; 109: 2778–2789. © 2012 Wiley Periodicals, Inc.
A case study in converting disposable process scouting devices into disposable bioreactors as a future bioprocessing tool
Authors: Vallejos José R. , Uplekar Shaunak , da Silva João F. , Brorson Kurt A. , Moreira Antonio R. , Rao Govind 
Published: May 2012

AbstractIn this study, we perform mass transfer characterization (kLa) on a novel mechanically driven/stirred Process Scouting Device, PSD, (SuperSpinner D 1000®, SSD) and demonstrate that this novel device can be viewed as disposable bioreactor. Using patch‐based optical sensors, we were able to monitor critical cell culture environmental conditions such as dissolved oxygen (DO) and pH in SSD for comparison to a 1 L standard spinner (SS) flask. We also coupled these mass transfer studies with mixing time studies where we observed relative high mixing times (5.2 min) that are typically observed in production scale bioreactors. Decreasing the mixing time 3.5‐fold resulted in 30% increase in kLa (from 2.3 to 3.0 h−1) and minimum DO level increased from 0% to 20% for our model hybridoma cell line. Finally, maximum viable cell density and protein titer stayed within ±20% of historical data, from our standard 5 L stirred bioreactor (Biostat®) operated under active DO control. Biotechnol. Bioeng. 2012; 109: 2790–2797. © 2012 Wiley Periodicals, Inc.
Liquid chromatography–mass spectrometry method for the analysis of 1,4‐dimethylpyridinium in rat plasma – application to pharmacokinetic studies
Authors: Szafarz Malgorzata , Szymura‐Oleksiak Joanna , Zakrzewska Agnieszka , Walczak Maria , Kus Kamil , Gonciarz Anna 
Published: May 2012

ABSTRACTA sensitive and specific liquid chromatography electrospray ionization–mass spectrometry method for determination of 1,4‐dimethylpyridinium (1,4‐DMP) in rat plasma has been developed and validated. Chromatography was performed on an Aquasil C18 analytical column (4.6 × 150 mm, 5 µm, Thermo Scientific, Rockford, IL, USA) with isocratic elution using a mobile phase containing acetonitrile and water with an addition of 0.1% of formic acid. Detection was achieved by an Applied Biosystems MDS Sciex (Concord, Ontario, Canada) API 2000 triple quadrupole mass spectrometer. Electrospray ionization was used for ion production. The limit of detection in the single ion monitoring mode was found to be 10 ng/mL. The limit of quantification was 50 ng/mL. The precision and accuracy for both within‐day and between‐day determination of 1,4‐dimethylpyridinium was 2.4–7.56 and 90.93–111.48%. The results of this analytical method validation allow pharmacokinetic studies to be carried out in rats. The method was used for the pilot study of the pharmacokinetic behavior of 1,4‐DMP in rats after intravenous administration. Copyright © 2012 John Wiley & Sons, Ltd.
Mammary analog secretory carcinoma of salivary gland: Cytologic diagnosis and differential diagnosis of an unreported entity
Authors: Pisharodi Latha 
Published: May 2012

AbstractMammary analog secretory carcinoma of salivary gland (MASC) is a recently described salivary gland tumor. Cytologic features of this neoplasm have hitherto not been described in the literature. This report discusses the fine‐needle aspiration findings of MASC and its differential diagnosis. Aspirates were richly cellular and consisted of clusters and singly dispersed cells with mild cytologic atypia. Cytoplasm was abundant and finely vacuolated. Signet ring cells were observed. Surgical resection confirmed the diagnosis of MASC. Diagn. Cytopathol. 2013. © 2012 Wiley Periodicals, Inc.