bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endocytosis and intracellular processing of platelet microparticles by brain endothelial cells
Authors: Faille Dorothée , El‐Assaad Fatima , Mitchell Andrew J. , Alessi Marie‐Christine , Chimini Giovanna , Fusai Thierry , Grau Georges E. , Combes Valéry 
Published: September 2011

AbstractPlatelet‐derived microparticles (PMP) bind and modify the phenotype of many cell types including endothelial cells. Recently, we showed that PMP were internalized by human brain endothelial cells (HBEC). Here we intend to better characterize the internalization mechanisms of PMP and their intracellular fate. Confocal microscopy analysis of PKH67‐labelled PMP distribution in HBEC showed PMP in early endosome antigen 1 positive endosomes and in LysoTracker‐labelled lysosomes, confirming a role for endocytosis in PMP internalization. No fusion of calcein‐loaded PMP with HBEC membranes was observed. Quantification of PMP endocytosis using flow cytometry revealed that it was partially inhibited by trypsin digestion of PMP surface proteins and by extracellular Ca2+ chelation by EDTA, suggesting a partial role for receptor‐mediated endocytosis in PMP uptake. This endocytosis was independent of endothelial receptors such as intercellular adhesion molecule‐1 and vascular cell adhesion molecule‐1 and was not increased by tumour necrosis factor stimulation of HBEC. Platelet‐derived microparticle internalization was dramatically increased in the presence of decomplemented serum, suggesting a role for PMP opsonin‐dependent phagocytosis. Platelet‐derived microparticle uptake was greatly diminished by treatment of HBEC with cytochalasin D, an inhibitor of microfilament formation required for both phagocytosis and macropinocytosis, with methyl‐β‐cyclodextrin that depletes membrane cholesterol needed for macropinocytosis and with amiloride that inhibits the Na+/H+ exchanger involved in macropinocytosis. In conclusion, PMP are taken up by active endocytosis in HBEC, involving mechanisms consistent with both phagocytosis and macropinocytosis. These findings identify new processes by which PMP could modify endothelial cell phenotype and functions.
SNARE complex‐mediated degranulation in mast cells
Authors: Woska Joseph R. , Gillespie Marc E. 
Published: September 2011

Introduction
SNARE function in membrane fusion events
Expression of SNAREs and SNARE complexes in mast cells
Functional studies implicating specific SNAREs or SNARE complexes in mast cell degranulation
RNA interference studies, SNARES and mast cells
Regulation of mast cell SNAREs
Conclusions

Mast cell function and dysregulation is important in the development and progression of allergic and autoimmune disease. Identifying novel proteins involved in mast cell function and disease progression is the first step in the design of new therapeutic strategies. Soluble N‐ethylmaleimide‐sensitive factor attachment protein receptors (SNAREs) are a family of proteins demonstrated to mediate the transport and fusion of secretory vesicles to the membrane in mast cells, leading to the subsequent release of the vesicle cargo through an exocytotic mechanism. The functional role[s] of specific SNARE family member complexes in mast cell degranulation has not been fully elucidated. Here, we review recent and historical data on the expression, formation and localization of various SNARE proteins and their complexes in murine and human mast cells. We summarize the functional data identifying the key SNARE family members that appear to participate in mast cell degranulation. Furthermore, we discuss the utilization of RNA interference (RNAi) methods to validate SNARE function and the use of siRNA as a therapeutic approach to the treatment of inflammatory disease. These studies provide an overview of the specific SNARE proteins and complexes that serve as novel targets for the development of new therapies to treat allergic and autoimmune disease.
Incidence of cervical cancer and age‐specific survival of small cell cervical carcinoma in Taiwan
Authors: HUANG CHAO‐YUAN , YOU SAN‐LIN , CHEN CHIEN‐JEN , CHENG WEN‐FANG , LUO HUI‐CHUN , HSIEH CHANG‐YAO 
Published: September 2011

AbstractObjective. To determine the incidence of cervical cancer and the age‐specific survival from small cell cervical carcinoma in Taiwan. Design. Retrospective study. Setting. Taiwan. Population. Women diagnosed with cervical cancer from 1991 to 2005. Methods. Analysis of data from the National Cancer Registration System and National Death Certification System. Main Outcome Measures. Incidence and age at diagnosis of cervical carcinoma and age‐specific and overall survival from small cell cervical carcinoma. Results. During the study period, 36 122 women were diagnosed with cervical cancer, and 81.8% had squamous cell carcinoma (SCC). For the periods 1991–1995, 1996–2000 and 2001–2005, the mean age at diagnosis increased from 53.9±13.3 to 55.0±14.9 and then to 56.7±14.7 years, respectively. The incidence of SCC decreased from 1991 to 2005. During the same period, non‐significant increases of adenocarcinoma and small cell carcinoma were noted. For SCC, occurrence peaked in 1991–1995 in patients 50–59 years of age. From 1996 to 2005, it peaked in patients 40–49 years of age. For cervical adenocarcinoma, occurrence peaked in patients 40–49 years of age, with a steady increase in this age group from 1991 to 2005. Occurrence of small cell cervical carcinoma peaked in the period 1991–1995 in patients 30–39 years of age. During the 15 years of the study, the overall mortality rate of the 198 patients with small cell cervical carcinoma was 65.7%. Conclusions. In Taiwan, the incidence of small cell cervical carcinoma and adenocarcinoma tended to increase, but the incidence of squamous cell cervical carcinoma significantly decreased during the period 1991–2005.
Labor progress among women attempting a trial of labor after cesarean. Do they have their own rules?
Authors: FARANESH RITA , SALIM RAED 
Published: September 2011

AbstractObjective. To examine the pattern of labor progression among second parous women who had a vaginal birth after a cesarean (VBAC) compared with primiparous and multiparous women who delivered vaginally. Design. Case–control study. Setting. University hospital in Israel, August 2005 through November 2008. Population. The cases were all 137 second parous women who had a VBAC during the study period. The control groups were: (1) 136 primiparous women; and (2) 137 women who had a second repeated vaginal delivery. Methods. Data were extracted from the electronic medical records at admission, labor charts and medical records at discharge. Controls were frequency‐matched to the study group for maternal age, gestational age and the work shift during which the delivery occurred. Main Outcome Measures. Length of the active phase of labor. Secondary outcomes were length of the second stage and incidence of vacuum extraction deliveries. Results. The length of the active phase was 176±116, 207±121 and 110±66 minutes in the study, first and second control groups, respectively. The active phase and stage 2 of the study group were significantly shorter than in the first control group (p=0.02 and p=0.007, respectively) and longer than in the second control group (p=0.001 in both stages). The incidence of vacuum deliveries was higher (p=0.001) in the study group (13.9%) compared with the second control group (1.5%). Conclusions. The results suggest that the pattern of labor progression among second parous women undergoing a trial of labor after cesarean differs from second parous women undergoing a repeated vaginal delivery. Recognizing this dissimilarity may help in avoiding unnecessary, occasionally deleterious, interventions.
Evaluation of immunohistochemical expression of p53, p21, p27, cyclin D1, and Ki67 in oral and oropharyngeal squamous cell carcinoma
Authors: Perisanidis Christos , Perisanidis Beata , Wrba Fritz , Brandstetter Anita , El Gazzar Sabine , Papadogeorgakis Nikolaos , Seemann Rudolf , Ewers Rolf , Kyzas Panayiotis A. , Filipits Martin 
Published: September 2011

J Oral Pathol Med (2012) 41: 40–46Background:  The purpose of this study was to evaluate whether the immunohistochemical expression of p53, p21, p27, cyclin D1, and Ki67 can predict therapy response and survival in patients with oral and oropharyngeal squamous cell carcinoma treated with preoperative chemoradiation.Methods:  Biomarker expression was evaluated by immunohistochemistry in formalin‐fixed, paraffin‐embedded pretreatment biopsies of 111 homogenously treated patients. We assessed the association between clinicopathological variables including response to neoadjuvant chemoradiotherapy as well as the survival of the patients and the expression of the biomarkers as both dichotomized (positive vs. negative) and continuous variables.Results:  Biomarker overexpression on the basis of pre‐selected cutoff points was seen in 66 of 111 (59%) cases for p53, in 77 (69%) for p21, in 48 (43%) for p27, in 81 (73%) for cyclin D1, and in 54 (49%) cases for Ki67, respectively. None of the examined biomarkers was able to predict response to neoadjuvant chemoradiotherapy or was associated with survival outcome. Post‐treatment pathologic TNM stage (P < 0.001), pathologic response (P < 0.001), and perineural invasion (P < 0.001) were the only factors having a significant effect on recurrence‐free survival. Post‐treatment pathologic N stage (P = 0.005), post‐treatment pathologic TNM stage (P < 0.001), pathologic response (P < 0.001), and perineural invasion (P = 0.001) had a significant impact on overall survival.Conclusions:  Our results suggest that the biomarkers p53, p21, p27, cyclin D1, and Ki67 have no impact on treatment response and survival in patients with oral and oropharyngeal cancer treated with preoperative chemoradiation.
The prevalence of dysplasia and malignant lip lesions in transplant patients
Authors: Nolan Anita , Girdler Nick M. , Seymour Robin A. , Mark Thomason J. 
Published: September 2011

J Oral Pathol Med (2012) 41: 113–118Background:  Solid organ transplant patients are at an increased risk of developing lip malignancies. The role of HLA mismatch as a risk factor for such changes has only been described in skin.Methods:  Lip lesions were evaluated in 403 solid organ transplant patients (immunosuppressed for at least 3 months) and findings compared to age and sex matched, otherwise healthy patients who acted as controls. HLA typing was provided for the transplant patients. All patients provided details of smoking history, alcohol consumption, skin type, as assessed by ease of burning to sunlight, and exposure to sunlight or other forms of ultraviolet radiation.Results:  Lip lesions were identified in 36 transplant patients and 29 were biopsied. Fourteen of the biopsies confirmed dysplastic or malignant changes. For the control patients, one lesion was identified as dysplastic. The prevalence of dysplastic and malignant lip lesions was significantly higher (P = 0.006) in the transplant patients when compared to controls. Risk factors for dysplastic/malignant changes in the transplant group included age (P = 0.01), smoking (P = 0.033) and HLA‐B mismatch (P = 0.001). Lip covering provided a significant reduction (P = 0.045) in the development of lip changes.Conclusion:  All transplant patients should be regularly screened for lip malignancies and consulted on smoking and sunlight exposure. HLA‐B mismatch does appear to make these patients more susceptible to dysplastic/malignant changes.
Assessment of melanocytic skin lesions with a high‐definition laser Doppler imaging system
Authors: Hunger Robert E. , Torre Rocco Della , Serov Alexandre , Hunziker Thomas 
Published: September 2011

BackgroundEarly detection is a major goal in the management of malignant melanoma. Besides clinical assessment many noninvasive technologies such as dermoscopy, digital dermoscopy and in vivo laser scanner microscopy are used as additional methods. Herein we tested a system to assess lesional perfusion as a tool for early melanoma detection.MethodsLaser Doppler flow (FluxExplorer) and mole analyser (MA) score (FotoFinder) were applied to histologically verified melanocytic nevi (33) and malignant melanomas (12).ResultsMean perfusion and MA scores were significantly increased in melanoma compared to nevi. However, applying an empirically determined threshold of 16% perfusion increase only 42% of the melanomas fulfilled the criterion of malignancy, whereas with the mole analyzer score 82% of the melanomas fulfilled the criterion of malignancy.ConclusionLaser Doppler imaging is a highly sensitive technology to assess skin and skin tumor perfusion in vivo. Although mean perfusion is higher in melanomas compared to nevi the high numbers of false negative results hamper the use of this technology for early melanoma detection.
Cutaneous resonance running time is decreased in psoriatic lesions
Authors: Lv Chengzhi , Song Shunpeng , Luo Wenhai , Elias Peter M. , Man Mao‐Qiang 
Published: September 2011

Background/aimsPsoriasis is characterized by lower stratum corneum (SC) hydration and dermal inflammation. Both SC hydration and cutaneous inflammation influence cutaneous resonance running time (CRRT). However, the characteristics of CRRT in psoriatic lesions are largely unknown. In the present study, we assessed whether changes in CRRT occur in psoriatic lesions in Chinese.MethodsA Reviscometer RVM600 and Corneometer CM 825 were used to measure CRRTs and SC hydration, respectively, in psoriatic lesions (psoriasis vulgaris) on the extensor of forearm in 111 subjects (58 men, 53 women), aged 23–80 years (50.42 ± 1.23 years). The contralateral uninvolved sites served as control.ResultsIn comparison with contralateral uninvolved sites, CRRTs in psoriatic lesions were reduced significantly in all directions. There was neither gender nor age difference in the extent of reduction in CRRTs. However, the reduction of CRRTs varied with measurement directions. Positive correlations of SC hydration with CRRTs were found at some directions in uninvolved and involved sites in young men whereas CRRTs in psoriatic lesions were not correlated with SC hydration in either aged or young women. Moreover, CRRT at 0–6 o'clock direction was positively correlated with SC hydration in involved sites of aged men.ConclusionCutaneous resonance running times are decreased in psoriatic lesions. Reduction of CRRTs varies with measurement directions, but not gender or age. Measurement of CRRTs could be another valuable approach to assess the severity of psoriasis and the efficacy of its treatment.
Primary succession trajectories on pumice at Mount St. Helens, Washington
Authors: del Moral Roger , Thomason Lindsay A. , Wenke Anthony C. , Lozanoff Natasha , Abata Mario D. 
Published: September 2011

AbstractQuestionsDoes vegetation become less variable over time? Do floristic trajectories converge during succession? Can either allogenic (external) or autogenic (internal) factors predict species patterns during succession?LocationPumice Plain of Mount St. Helens (46.23449°N; 122.15929°W, 1230 m a.s.l.), which was sterilized in 1980 by a direct volcanic blast, then buried in pumice.MethodsWe monitored a grid of 200 100‐m2 contiguous plots annually (1989–2010) and classified the 2010 data into five community types (CTs). We characterized plots using external (e.g. distance from relict sites) and internal (e.g. moisture) factors and clustered plots by position and habitat factors to assess trajectories. We used redundancy analysis (RDA) to assess relationships between vegetation and these variables over time. Mantel tests, similarity changes and detrended correspondence analysis (DCA) were used to evaluate successional trends.ResultsFive CTs were weakly related to habitats at this scale. Over time, vegetation became more homogeneous and successional trajectories of plots clustered by position and habitat factors became more similar or developed in parallel. The succession rate slowed fitfully as persistent species became dominant, while Lupinus fluctuated. Mantel tests indicated that relationships between species and environment were maximized using only autogenic factors. RDA found that autogenic factors were stronger than allogenic ones, while explained variance did not increase after 1992 and factor correlations to species stabilized by 1996.ConclusionsThe strength of links between vegetation and environment were weak at this scale, yet explained variation stabilized early in succession. As substantial vegetative variation persisted and convergence was far from complete due to weak links to habitat factors, habitat variation and stochastic establishment should be considered by vegetation managers.
Management of Provisional Restorations' Deficiencies: A Literature Review
Authors: PATRAS MICHAEL , NAKA OLGA , DOUKOUDAKIS SPYRIDON , PISSIOTIS ARGIRIS 
Published: September 2011

ABSTRACTProvisional restorations are designed in order to protect oral structures and promote function and esthetics for a limited period of time, after which they are to be replaced by a definite prosthesis. They play a particular role in diagnostic procedures and continued evaluation of the treatment plan, as they should resemble the form and function of the definite rehabilitation that they precede. Therefore, interim treatment should satisfy the criteria of marginal adaptation, strength, and longevity. In complicated treatment plans that intend to last for extended periods of time, the function of provisional prostheses involves the possibility of relining, modification, or repair. These adjustments raise considerations regarding the strength of the resultant bond. Chemical composition of the base and repair material, surface characteristics of fracture parts, and time elapsed since the initial set of the rehabilitation should be considered in the decision of the appropriate repair material and technique. Proper pretreatment of the provisional components' surfaces is essential to ensure bonding as well.The purpose of this article is to illustrate the management of provisional restorations' deficiencies. This article highlights possible failures of custom‐fabricated provisional restorations, describes methods to prevent their occurrence, and discusses clinical techniques for their management. Finally, the proper combination of materials and surface preparation to achieve the optimum treatment outcomes are presented.CLINICAL SIGNIFICANCEProvisional restorations' failures and other deficiencies are encountered by clinicians on a daily basis. Adequate laboratory techniques and material combinations presented herein may contribute to their efficient and predictable modifications and repairs.(J Esthet Restor Dent 24:26–39, 2012)
Sexual Behaviors and Situational Characteristics of Most Recent Male-Partnered Sexual Event among Gay and Bisexually Identified Men in the United States
Authors: Rosenberger Joshua G. , Reece Michael , Schick Vanessa , Herbenick Debby , Novak David S. , Van Der Pol Barbara , Fortenberry J. Dennis 
Published: September 2011

ABSTRACT

Introduction
Recent nationally representative studies documenting event-level sexual behavior have included samples that are predominantly heterosexual, resulting in limited information on the sexual repertoire of gay and bisexually identified men.

Aim
This study sought to document the sexual behaviors that gay and bisexually identified men report during their most recent male-partnered sexual event and to describe the situational characteristics and participants' evaluation of these events.

Methods
Via an internet-based survey, data were collected from 24,787 gay and bisexually identified men (ages 18–87 years) from 50 US states and the District of Columbia.

Main Outcome Measures
Measures included items related to sociodemographics, recent sexual behavior history, situational characteristics, orgasm, and ratings of arousal and pleasure.

Results
Participants' mean age was 39.2 years; ethnicities included white (84.6%), Latino (6.4%), and African American (3.6%); and most men (79.9%) identified as homosexual. The most commonly reported behavior was kissing a partner on the mouth (74.5%), followed by oral sex (72.7%), and partnered masturbation (68.4%). Anal intercourse occurred among less than half of participants (37.2%) and was most common among men ages 18–24 (42.7%). Sex was most likely to occur in the participant's home (46.8%), with less frequently reported locations including hotels (7.4%) and public spaces (3.1%). The number of behaviors engaged in during last sexual event varied with most (63.2%) including 5–9 different sexual behaviors.

Conclusions
These data provide one of the first examinations of sexual behaviors during the most recent male-partnered sexual event among gay and bisexually identified men in the United States. Findings from this study suggest that gay and bisexually identified men have a diverse sexual repertoire and that partnered sexual behaviors are not limited solely to acts of penile insertion.
Use of Pelvic Floor Ultrasound to Assess Pelvic Floor Muscle Function in Urological Chronic Pelvic Pain Syndrome in Men
Authors: Davis Seth N. , Morin Melanie , Binik Yitzchak M. , Khalife Samir , Carrier Serge 
Published: September 2011

ABSTRACTIntroductionAn important cause or maintaining factor for pain in Urological Chronic Pelvic Pain Syndrome (UCPPS) may be pelvic floor muscle (PFM) dysfunction, which may also be implicated in sexual dysfunction and influenced by psychosocial factors. Pelvic floor ultrasound is a noninvasive, reliable, and relatively simple method to assess PFM morphology and function and can be assessed by the anorectal angle (ARA) and levator plate angle (LPA).AimsThe aim of the present study was to examine PFM morphology in men with UCPPS as compared with controls and to examine the correlation with pain and psychosocial measures.MethodsOur participants were 24 men with UCPPS and 26 controls. A GE Voluson E8 ultrasound probe was placed on the perineum, and three-dimensional images were taken at rest and during PFM contraction.Main Outcome MeasuresThe main outcomes were ARA and LPA at rest and contraction. Participants also completed the National Institute of Health (NIH) Chronic Prostatitis Symptom Index, Male Sexual Health Questionnaire, State Anxiety Inventory, and Pain Catastrophizing Scale.ResultsMen with UCPPS had more acute ARAs than controls both at rest and during contraction. The two groups did not differ in LPA at rest; however, men with UCPPS had significantly more acute angles during contraction and LP excursion. Acute ARAs were positively correlated with greater pain report and sexual dysfunction. Anxiety was correlated with more acute ARAs and more obtuse LPAs.ConclusionThree implications can be drawn from the findings. First, ARA at rest and during contraction as well as LP angle during contraction and LPA excursion separates men with UCPPS from controls. Second, ARA at rest and during contraction was correlated with pain and sexual dysfunction, while LPA at rest was related to anxiety. Third, pelvic floor ultrasound has the potential to be a useful and objective method of assessing PFM morphology in UCPPS.