bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overexpression of the Superoxide Anion and NADPH Oxidase Isoforms 1 and 4 (NOX1 and NOX4) in Allergic Nasal Mucosa
Authors: Moon Joon Hwan , Kim Tae Hoon , Lee Heung Man , Lee Seung Hoon , Choe Whan , Kim Ha Kyun , Lee Jung Hoon , Oh Kyoung Ho , Lee Sang Hag 
Published: February 2010

Background The purpose of this study was to investigate the expression and distribution of superoxide anion, NADPH oxidase (NOX)1, and NOX4 in healthy, allergic nasal mucosa and nasal polyps to evaluate the possible influence of oxidative stress on the development of allergic rhinitis and nasal polyps. Methods The expression and distribution of superoxide anion, NOX1 and NOX4 were evaluated in healthy and allergic nasal mucosa and nasal polyps, using dihydroethidium fluorescence, semiquantitative reverse transcriptase-polymerase chain reaction, immunohistochemistry, and Western blot. Results NOX1 and NOX4 were localized mainly in the epithelial layer, submucosal glands, vascular endothelium, and inflammatory cells in healthy and allergic nasal mucosa and nasal polyps. The cellular source that generated superoxide anion is also localized in the epithelial cells, submucosal glands, vascular endothelium, and inflammatory cells, demonstrating the similar sites of expression of NOX1 and NOX4 in healthy and allergic nasal mucosa and nasal polyps. NOX1 and NOX4 mRNA and proteins and superoxide anions had increased levels of expression in allergic nasal mucosa and nasal polyps compared with healthy nasal mucosa. Conclusions These results indicate that NOX1 and NOX4 may play an important role in reactive oxygen species production, contributing to the oxidative stress in allergic rhinitis and nasal polyp tissues.
Liquid-Based Cytology in Diagnosing Nasopharyngeal Carcinoma
Authors: Jan Yee-Jee , Chen Su-Ju , Wang John , Jiang Rong-San 
Published: February 2010

Background Liquid-based cytology (LBC) has achieved great success in cytological diagnosis of various cancers when compared with conventional smear methods. However, its application in diagnosing nasopharyngeal carcinoma (NPC) has never been studied. Methods Eighty-four consecutive patients who underwent nasopharyngeal biopsy for suspicious NPC or a nasopharyngeal mass under nasopharyngoscopy were enrolled in this prospective study. Brush samples were taken from the same site before punch biopsy and processed with the Thin Prep test. Results The adequacy, accuracy, sensitivity, specificity, false-negative rate, and false-positive rate of LBC in diagnosing NPC were 92.9% (78 of 84), 93.6% (73 of 78), 84.2% (16 of 19), 96.6% (57 of 59), 15.8% (three of 19), and 3.4% (two of 25), respectively. There were four inadequate specimens from patients with NPC and two inadequate ones from those without NPC. Conclusions Our study showed that the adequacy, accuracy, sensitivity, specificity, and diagnostic rate of LBC were equivalent to those using conventional smear methods. Although the diagnostic rate of NPC was lower using brush cytology than by punch biopsy, further improvements in the sampling technique could make brushing cytology a potential tool for NPC screening.
Serum Levels of Resistin in Allergic Rhinitis and its Relationship with Disease Severity
Authors: Hsueh Kai-Chung , Lin Ching-Yuang , Lin Ying-Ju 
Published: February 2010

BackgroundResistin, a recently discovered adipokine, has been shown to have proinflammatory properties in humans. The purpose of this study was to evaluate serum resistin levels in children with allergic rhinitis (AR) and to investigate its association with clinical disease severity, parameters of atopy and pro-/anti-inflammatory cytokines.MethodsA case-controlled study was performed on 50 pediatric patients with persistent AR and 30 healthy controls with similar age, sex, and body mass index. Serum levels of resistin, parameters of atopy, and cytokines were analyzed. The nasal symptom scores were evaluated and patients were classified into mild (n = 20) and moderate-severe (n = 30) persistent AR.ResultsSerum levels of resistin were significantly increased in children with AR compared with controls (p < 0.001). Serum levels of resistin went hand in hand with disease severity as they were significantly higher in moderate-severe than mild persistent AR. In addition, they correlated positively with nasal symptom scores (r = 0.74; p < 0.001). A significant positive correlation was observed between serum levels of resistin and IL-6 (r = 0.358; p = 0.011).ConclusionPatients with persistent AR were found to have higher serum levels of resistin, and resistin levels increased with the progress of disease severity. Resistin may represent a novel link between inflammation and AR.
KTP/532 YAG Laser Treatment for Allergic Rhinitis
Authors: Yaniv Eitan , Hadar Tuvia , Shvero Jacob , Tamir Rami , Nageris Ben 
Published: February 2010

Background The purpose of this study was to investigate the efficacy of the KTP/532 YAG laser to reduce nasal congestion and discharge in patients with allergic rhinitis. Methods Forty-eight patients with symptoms of allergic rhinitis were treated with the KTP/532 laser. All had positive skin tests for common allergens. Treatments were provided on an ambulatory basis in one to three sessions under local anesthesia with lidocaine nose spray. Outcome was determined by daily symptom reports and regular endoscopy examination and interviews for 12 months. Results Treatment was very well tolerated. There were no major side effects. At examination after 1 year, nasal obstruction was improved in 69% and nasal discharge in 40% of cases. Conclusion The KTP/532 YAG laser is effective for the treatment of nasal obstruction and discharge. Comparison with other techniques showed it to be the most effective in reducing nasal discharge. It can be done as an office procedure and does not damage the nasal mucous membrane. The KTP/532 YAG laser is effective as an additional treatment for patients refractory to medication.
Nasal Physiological Reactivity of Subjects with Nonallergic Rhinitis to Cold Air Provocation: A Pilot Comparison of Subgroups
Authors: Shusterman Dennis J. , Tilles Stephen A. 
Published: February 2010

Background Noninfectious nonallergic rhinitis (NINAR) is characterized by self-reported hyperreactivity to nonspecific physical or chemical stimuli. The relationship between these two classes of triggers is not well established, however. We compared NINAR subjects with predominantly physical or chemical triggers versus normal controls with respect to subjective (symptomatic) and objective (obstructive) responses to cold, dry air challenge. Methods We studied 14 NINAR subjects and 10 normal controls. Exposures consisted of 15 minutes of cold dry air (0°C/5% RH) or warm moist air (25°C/50% RH) on two separate days a week apart. Subjects rated symptoms using visual analog scales and had their nasal airway resistance measured at baseline, immediately after, and at 15-minute intervals for 1 hour postexposure. Results The majority of NINAR subjects reported physical triggers as more troublesome than chemical. Immediately postprovocation, the mean net proportional change in nasal airway resistance from baseline was +0.18 in NINAR (physical), +0.05 in NINAR (chemical), and –0.01 in control subjects (NS). However, a pooled linear regression by number of physical triggers (0–5) revealed a 7.5% increase in cold air–induced nasal airway resistance per trigger reported (p < 0.05). Similarly, raising the criterion number of physical triggers from ≥1 to ≥2 also distinguished NINAR subjects from controls in a bivariate analysis. Conclusion Either considering self-reported physical triggers as a continuous scale (0–5) or requiring more physical triggers (≥ 2 rather than ≥1) to define NINAR successfully predicts objective nasal reactivity to cold air provocation.
Prophylactic i.v. Antibiotics in Functional Endoscopic Sinus Surgery: Trends and Attitudes of the American Rhinologic Society Membership
Authors: Chandra Rakesh K. , Conley David B. , Kern Robert C. 
Published: February 2010

Background Use of antibiotic prophylaxis in otolaryngology–head and neck surgery is a practice that exhibits significant variation among practitioners, and trends vary by subspecialties within the field. The objective of this study was to assess these habits among otolaryngologists with special interest or experience in functional endoscopic sinus surgery (FESS). Methods Attendees of the 2008 Annual Meeting of the American Rhinologic Society were surveyed regarding practice patterns and attitudes concerning the use of prophylactic i.v. antibiotics in FESS. Results Surveys were returned from 49 attendees, and within this sample, i.v. antibiotic prophylaxis was used by 28 (57%) and was not in 21 (43%). Among those who used prophylaxis, only 2/28 (7%) did so because they felt there was strong evidence to support such a practice, while 26/28 (93%) used prophylactic antibiotics despite the opinion that there is no solid evidence in support of this. The most common antibiotic chosen empirically was a cephalosporin (25/28; 89%). There was no significant difference between those who did and did not use prophylactic i.v. antibiotics with regard to number of years in practice or practice situation (full-time academic, academic affiliated private, solo private, and group private). Conclusion Opinion of the respondents suggests there is no solid empiric evidence supporting the use of prophylactic i.v. antibiotics in FESS. Nonetheless, this practice is more common than not. Possible explanations for these observations are explored in the context of new pay for performance quality measures.
Matrix Metalloproteinase-9 Promoter Gene Polymorphism (–15620T) in Nasal Polyposis
Authors: Erbek Selim S. , Yurtcu Erkan , Erbek Seyra , Sahin Feride I. 
Published: February 2010

Background Expression of matrix metalloproteinase (MMP)-9 increases in nasal polyp tissues. However, the impact of MMP-9 genotypes on the development of nasal polyposis (NP) is unknown. The aim of this study was to examine a potential association of MMP-9 promoter gene polymorphism with the development of NP. Methods A prospective and case–control study was performed on 93 patients with NP and 115 controls without sinonasal disease. Genotypes of MMP-9 (–1562C>T) were identified by restriction fragment length polymorphism analyses after polymerase chain reaction. Results The frequency of – 1562CT genotype of MMP-9 was significantly high in NP patients with aspirin-induced asthma (p = 0.014). Distribution of T allele was significantly high in NP patients with aspirin-induced asthma (p = 0.013). MMP-9 genotypes were not associated with gender or the presence of atopy. Conclusion In this study, MMP-9 – 1562CT genotype was associated with susceptibility to NP in aspirin-induced asthmatic patients. Because this report is a population-based study, further research should be performed on larger study subjects to reveal the precise role of MMP-9 promoter gene polymorphism in the development of NP.
The Two Holes Syndrome
Authors: Mladina Ranko , Vuković Katarina , Poje Gorazd 
Published: February 2010

Background Defects in the fontanel region of the lateral nasal wall have been described in the literature as “accessory” or “secondary” ostia. The authors consider them a sign of chronic maxillary sinusitis. Along with mucus recirculation between the natural ostium and the fontanel defect, we call it the Two Holes Syndrome. The aim of this study was to determine the incidence of fontanel defects in patients with chronic rhinosinusitis (CRS) and in healthy subjects. Methods Eighty-eight hundred seventy-nine outpatients with CRS were examined by means of nasal fiberendoscopy. The control group consisted of 1442 healthy volunteers with no previous history of CRS. Results Defect in the posterior fontanel was found in 1713/8879 CRS patients (19.3%). It was bilateral in 1165 cases (68.03%). Defect in the anterior fontanel was found in 54 patients (0.61%). The circulating mucus ring was identified in 162 patients with fontanel defects (9.17%). The defect in the posterior fontanel was found in 7/1442 healthy volunteers (0.48%). It was never bilateral, the circulating mucus was not observed and defect in the anterior fontanel was not found. Conclusion Posterior fontanel defects were found more frequently in CRS patients than in healthy subjects. These defects have been clinically related to chronic infection of the maxillary sinus and should not be called “accessory” or “secondary” ostia. CRS with defects of the fontanel region and mucus recirculation can promote a number of health disturbances (chronic postnasal drip, headache, and cough). We named this entity the Two Holes Syndrome.
Mastoid Bone as a New Graft Material in Rhinoplasty
Authors: Sadooghi Mahmood , Kouhi Ali 
Published: February 2010

Background The management of certain nasal deformities, especially after prior rhinoplasty, may require grafting material. In this study we describe the use of mastoid bone as a viable and low morbidity autologous graft. Methods Mastoid bone was used for nasal augmentation, smoothing dorsal nasal irregularities, or augmentation of radix. Candidates for mastoid bone graft were patients undergoing primary rhinoplasty suffering from low radix or saddle nose deformity and candidates for secondary rhinoplasty suffering from prior overresection of the osteocartilaginous structures. Results Fifty-six patients met the study criteria. Eighteen patients underwent secondary rhinoplasty and suffered from prior overresection of osteocartilaginous structures and 38 patients underwent primary rhinoplasty. Of these, 18 patients had a low radix and 20 patients had a saddle nose deformity. Follow-up was 6–49 months (mean, 23 months). All cases resulted in an augmented straightened nasal dorsum, increased tip projection, and adjusted radix. There were no cases of graft infection. In two cases the graft was displaced requiring revision. The amount of graft absorption even after 2 years follow-up was acceptable. There were no donor site complications. Conclusions The mastoid bone graft provides adequate autologous bone in most cases of primary or revision rhinoplasty. The donor site carries low morbidity and a well camouflaged scar. It is easily accessible especially for the otolaryngologist who is accustomed to operating on the mastoid bone.
Anatomic Variations of Sphenopalatine Artery and Minimally Invasive Surgical Cauterization Procedure
Authors: Midilli Rasit , Orhan Mustafa , Saylam Canan Y. , Akyildiz Serdar , Gode Sercan , Karci Bulent 
Published: February 2010

Background Sphenopalatine artery (SPA) ligation or cauterization stands to be one of the most common management options of refractory epistaxis. Ramification pattern of SPA as it passes through sphenopalatine foramen (SPF) has not been clearly established. The aim of this study is to investigate situations in which middle meatal approach may fail due to anatomic variations of SPA and to define a minimally invasive surgical cauterization procedure. Anatomic variations of SPA were determined by microdissection of 20 adult sagittally cross-sectioned head specimens. Methods Branching characteristics of SPA and its anatomic relations were evaluated and anatomic variations were noted. Results SPA was generally (80%) forming branches within SPF before entering into the nasal cavity. In 20% of the specimens, SPF was located superior to the horizontal lamella of the middle turbinate, and accessory foramen was present in 10%. In 10% of the cases, the posterior lateral nasal branch was situated as two branches in a deep sulcus in the middle meatus. Conclusion The ramification pattern of SPA can not be fully exposed without resection of the posterior part of the middle turbinate via the middle meatal approach. Two-step procedures are advocated in reducing failure rates. Previously defined two-step procedures are relatively invasive. A less invasive procedure is defined based on the variations of SPA and SPF.
Rosai-Dorfman Disease of the Nasal Cavities: A CO2 Laser Excision
Authors: Belcadhi Malek , Bellakhdhar Mouna , Sriha Badreddine , Bouzouita Kamel 
Published: February 2010

Background Rosai-Dorfman disease (RDD) is a rare nonneoplastic histiocytic proliferative disorder characterized by painless massive lymphadenopathy. Extranodal manifestations frequently occur in the head and neck area. Methods We report the clinical and histological features of nasal cavities and cervical lymph node RDD in a 72-year-old woman, who complained of major nasal obstruction and hyposmia. Results Nasal endoscopy revealed polypoid masses arising from the turbinates; the septum was intact. Immunohistochemical analysis revealed staining for S-100 protein, CD68, and alpha-1 antitrypsin. The presence of the Epstein-Barr virus was revealed by serology and also by in situ hybridization. The general examination and systemic evaluation did not find other extranodal localization. Conclusions Because the disease is known for not responding to local or systemic steroids, CO2 laser excision was used to clear the nasal obstruction with a satisfactory result. This is, to the best of our knowledge, the first description of the use of laser excision for nasal cavities RDD.
A Systematic Review of Outcomes after Dacryocystorhinostomy in Adults
Authors: Leong Samuel C. , MacEwen Caroline J. , White Paul S. 
Published: February 2010

Background This study was designed to systematically review the clinical outcomes of dacryocystorhinostomy (DCR) surgery. Data sources included PubMed for English language literature from January 1966 to December 2008 combined with a manual review of citations within article bibliographies. Methods Citations acquired from the targeted search were filtered independently by two researchers. Relevant articles were reviewed to obtain information including interventions and outcome measures. The surgical techniques were categorized into external DCR (EX-DCR), endonasal laser-assisted DCR (LA-DCR), and nonlaser endoscopic endonasal DCR techniques (EN-DCR). Articles were then assigned level-of-evidence grades as defined by the Oxford Center for Evidence-Based Medicine. Results A total of 73 studies that fulfilled the inclusion criteria were analyzed. Of these, 68 were graded as level 4, 11 were graded as level 3b, and 1 was graded at level 2b evidence. A total of 4800 patients were pooled, from which 4921 DCRs were performed. All studies reported success during the follow-up period, although the outcome measures used were not consistent in the studies. Success varied between 65 and 100% after EX-DCR compared with EN-DCR, which varied from 84 to 94%. The success rate of LA-DCR varied widely between 47 and 100%. There was low evidence base to support the use of silicone stent to improve surgical success. Most studies did not show significantly improved outcomes with an antimetabolite. The overall intra- and postoperative complication rates were 1.0 and 6%, respectively. Conclusions DCR is an effective and safe method for the treatment of nasolacrimal obstruction. Meta-analysis of outcomes was not feasible because of the heterogenous patient groups and outcome measures used. Nonetheless, the literature provides considerable levels 3 and 4 evidence to support DCR surgery in adults. Outcomes after EN-DCR and EX-DCR were comparable. The failure rate for LA-DCR was higher.
Association of the CD14 Gene Polymorphism C-159T with Allergic Rhinitis
Authors: Han Demin , She Wenyu , Zhang Luo 
Published: February 2010

Background Altered expression of the cluster differentiation antigen, CD14, may affect the proportion of T-helper type 1 (Th1) to Th2 cells, thereby influencing the immunoglobulin E (IgE) response and associated inflammatory phenotype in allergic conditions. Therefore, the gene encoding CD14 is considered as a positional candidate gene for atopy. Recently, a functional C-159 T polymorphism has been described in the promoter region of the gene and has been associated with increased gene expression and atopic phenotypes in various ethnic populations. We evaluated the relationship between the C-159 T polymorphism in the promoter of the CD14 gene and the total serum IgE level in Chinese adult patients with allergic rhinitis. Methods Polymerase chain reaction with restriction fragment length polymorphism (RFLP) analysis was used to determine the C-159 T polymorphism in subjects with allergic rhinitis (n = 92) and nonallergic disease controls (n = 72). Total serum IgE was also measured. Results In the allergic rhinitis group, the frequency of the TT homozygotes was significantly higher than for the control group (p < 0.05). In both groups, the C-159 T polymorphism is not associated with serum IgE levels. Conclusions TT homozygotes are more common in adult patients with allergic rhinitis among the Chinese population.
Incidence of Staphylococcal Colonization and of the 753Q Toll-like Receptor 2 Variant in Nasal Polyposis
Authors: Sachse Florian , Becker Karsten , Rudack Claudia 
Published: February 2010

Background The impact of Staphylococcus aureus on the development of chronic sinusitis with nasal polyps (nasal polyposis [NP]) is a controversial discussion because different S. aureus colonization rates have been reported. Aside from the presence of a microbial stimulus, elements of innate immunity such as Toll-like receptors (TLRs) and/or impaired TLR function could be relevant for the development of this disease. Because the 753Q TLR2 variant may predispose to staphylococcal infection, we simultaneously analyzed staphylococcal colonization and the R753Q TLR2 single nucleotide polymorphism (SNP) in NP. Methods Sixty-eight patients with NP (47 men and 21 women; mean age [±SD], 51.8 years [16.3]) and 51 controls (32 men and 19 women; mean age [±SD], 36.3 years [12.2]) were included. Patient characteristics studied included status of allergy, asthma, aspirin intolerance, and endoscopic and CT polyp score. For detection of bacteria, standard procedures of bacteriology and 16S rRNA gene sequencing were used. The R753Q TLR2 polymorphism was studied by allelic discrimination assay. Results Overall, 128 isolates were cultured from 68 NP specimens, with Staphylococcus epidermidis and S. aureus being the most frequent bacterial isolates. Other bacterial species were infrequently detected. Fifty-nine isolates were cultured from 51 controls. Similarly, S. epidermidis and S. aureus were the most frequent bacterial isolates. S. aureus colonization was significantly increased in NP (p < 0.05). However, SNP genotyping results showed no association of the 753Q TLR2 variant with NP. Conclusions Although S. aureus detection was increased in NP, nasal polyp pathology is not related to the 753Q TLR2 variant.