bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long non‐coding RNAs as key regulators of neurodegenerative protein aggregation
Authors: Xu Qi , Liu Dan , Zhu Ling‐Qiang , Su Ying , Huang He‐Zhou 
Published: February 2025

AbstractThe characteristic events in neurodegenerative diseases (NDDs) encompass protein misfolding, aggregation, accumulation, and their related cellular dysfunction, synaptic function loss. While distinct proteins are implicated in the pathological processes of different NDDs, the process of protein misfolding and aggregation remains notably similar across various conditions. Specifically, proteins undergo misfolding into beta‐folded (β‐folded) conformation, resulting in the formation of insoluble amyloid proteins. Despite advancements in comprehending protein aggregation, certain facets of this intricate process remain incompletely elucidated. In recent years, the concept that long non‐coding RNAs (lncRNAs) contribute to protein aggregation has gained recognition. LncRNAs influence the formation of protein aggregates by facilitating protein overexpression through the regulation of gene transcription and translation, inhibiting protein degradation via lysosomal and autophagic pathways, and targeting aberrant modifications and phase transitions of proteins. A better understanding of the relationship between lncRNAs and aberrant protein aggregation is an important step in dissecting the underlying molecular mechanisms and will contribute to the discovery of new therapeutic targets and strategies.Highlights
NDDs are marked by protein misfolding, aggregation, and accumulation, leading to cellular dysfunction and loss of synaptic function.
Despite different proteins being involved in various NDDs, the process of misfolding into β‐folded conformations and forming insoluble amyloid proteins is consistent across conditions.
The role of lncRNAs in protein aggregation has gained attention, as they regulate gene transcription and translation, inhibit protein degradation, and target aberrant protein modifications.
Understanding the link between lncRNAs and protein aggregation is crucial for uncovering molecular mechanisms and developing new therapeutic targets.
Parallel changes in cognition, neuropsychiatric symptoms, and amyloid in cognitively unimpaired older adults and those with mild cognitive impairment
Authors: De Vito Alyssa N. , Kunicki Zachary J. , Joyce Hannah E. , Huey Edward D. , Jones Richard N. ,   for the Alzheimer's Disease Neuroimaging Initiative
Published: February 2025

AbstractINTRODUCTIONAlzheimer's disease (AD) diagnosis centers on cognitive impairment despite other early indicators like neuropsychiatric symptoms (NPSs) and amyloid beta (Aβ) accumulation. This study examined how cognition, NPS, and Aβ changes are interrelated over time in individuals without dementia.METHODSParticipants were 1247 individuals from the Alzheimer's Disease Neuroimaging Initiative (ADNI)‐2 and ‐3 cohorts with at least 48 months of follow‐up. Cognitive domains were assessed via ADNI composite measures, NPS via the neuropsychiatric inventory, and Aβ via standardized uptake value ratio (SUVR) composite scores. Co‐occurring changes were evaluated with parallel process models.RESULTSNPS was longitudinally associated with performance in each cognitive domain. Negative baseline Aβ‐cognition associations were observed in three cognitive domains. No Aβ‐NPS associations were observed.DISCUSSIONThis study demonstrated strong longitudinal relationships between NPS and cognition in preclinical and prodromal stages of AD. Future studies should incorporate NPS into models of disease trajectories to improve early detection and prediction of disease progression.Highlights
Co‐occurring changes in Aβ, cognition, and neuropsychiatric symptoms are understudied.
We found relationships between neuropsychiatric symptoms and cognition.
We found baseline, but not longitudinal, Aβ and cognition associations.
Changes in neuropsychiatric symptoms should be included in early detection models of ADRD.
Age at menopause and cognitive function and decline among middle‐aged and older women in the China Health and Retirement Longitudinal Study, 2011–2018
Authors: Guo Muqi , Wu Yingyan , Gross Alden L. , Karvonen‐Gutierrez Carrie , Kobayashi Lindsay C. 
Published: February 2025

AbstractINTRODUCTIONChinese women experience higher dementia rates than men, yet sex‐specific risk factors are understudied. We examined how menopause age affects cognitive function and decline in aging Chinese women.METHODSData were from 7419 postmenopausal women 45–101 years of age at baseline in the China Health and Retirement Longitudinal Study (CHARLS; 2011–2018). Menopause age was categorized using clinical cutoffs (<40, 40–44, 45–49, 50–55, >55 years). Cognitive function was assessed with neuropsychological tests up to four times over 7 years, and associations were analyzed using multivariable‐adjusted linear mixed‐effects regression.RESULTSCompared to menopause at 50–55 years (3661/7419; 49.3%), premature (<40; 235/7419; 3.2%), early (40–44; 623/7419; 8.4%), and late menopause (>55; 366/7419; 4.9%) were associated with lower baseline cognitive scores. Although the rate of cognitive decline did not differ significantly across menopause age groups, late menopause showed a trend toward faster decline.DISCUSSIONCognitive health interventions should consider extreme menopausal age as a risk factor.Highlights
Extreme menopausal ages—premature (<40), early (40–44), and late (>55)—are linked to lower baseline cognition versus menopause ages 50–55, persisting over 7 years.

Cognitive disadvantage for late menopause (>55) versus 50–55 tends to increase over time.
Health interventions should consider extreme menopause ages in women's cognitive health.
Closed‐Loop Recyclable Lithium and Sodium Conducting Covalent Adaptable Networks
Authors: Chen Hongxuan , Wayment Lacey J. , Jiang Huan , Lei Zepeng , Huang Shaofeng , Ley Ashley , Yue Zihao , Yuan Zhehao , Jin Yinghua , Yu Kai , Zhang Wei 
Published: February 2025

AbstractWithin the past two decades, covalent adaptable networks (CANs) have emerged as a novel class of dynamically crosslinked polymers, combining the benefits of thermosets and thermoplastics. Although some CANs with charged side chains have been reported, CANs with negatively charged backbones remain very limited. The integration of permanent charge into the backbones upon their formation could open up important new applications. Here, we introduce a series of aliphatic spiroborate‐linked ionic covalent adaptable networks (ICANs), representing a new category of dynamic ionomer thermosets. These ICANs were synthesized using a catalyst‐free, scalable, and environment‐friendly method. Incorporating lithium or sodium as counter cations in these networks yielded promising ion conductivity without the need of plasticizers. The dynamic nature of the spiroborate linkages in these materials allows for rapid reprocessing and recycling under moderate conditions. Furthermore, their potential as flexible solid‐state electrolytes is demonstrated in a device that maintained robust conducting performance under extreme physical deformation, coupled with effective self‐healing properties. This research opens new possibilities for future development of dynamic ionomer thermosets and their potential applications in flexible electronic devices.
Enzyme Controlled Transient Phospholipid Vesicles for Regulated Cargo Release
Authors: Venugopal Akhil , Ghosh Subhadip , Calò Annalisa , Tuveri Gian Marco , Battaglia Giuseppe , Kumar Mohit 
Published: February 2025

AbstractMetabolism in biological systems involves the continuous formation and breakdown of chemical and structural components, driven by chemical energy. In specific, metabolic processes on cellular membranes result in in situ formation and degradation of the constituent phospholipid molecules, by consuming fuel, to dynamically regulate the properties. Synthetic analogs of such chemically fueled phospholipid vesicles have been challenging. Here we report a bio‐inspired approach for the in situ formation of phospholipids, from water soluble precursors, and their fuel driven self‐assembly into vesicles. We show that the kinetic competition between anabolic and catabolic‐like reactions leads to the formation and enzymatic degradation of the double‐tailed, vesicle‐forming phospholipid. Spectroscopic and microscopic analysis demonstrate the formation of transient vesicles whose lifetime can be easily tuned from minutes to hours. Importantly, our design results in the formation of uniform sized (65 nm) vesicles simply by mixing the precursors, thus avoiding the traditional complex methods. Finally, our sub‐100 nm vesicles are of the right size for application in drug delivery. We have demonstrated that the release kinetics of the incorporated cargo molecules can be dynamically regulated for potential applications in adaptive nanomedicine.
Tuning Atomically Dispersed Metal Sites in Nanozymes for Sensing Applications
Authors: Liu Mingwang , Xu Weiqing , Tang Yinjun , Wu Yu , Gu Wenling , Du Dan , Lin Yuehe , Zhu Chengzhou 
Published: February 2025

AbstractNanozymes with atomically dispersed metal sites (ADzymes), especially single‐atom nanozymes, have attracted widespread attention in recent years due to their unique advantages in mimicking the active sites of natural enzymes. These nanozymes not only maximize exposure of catalytic sites but also possess superior catalytic activity performance, achieving challenging catalytic reactions. These advantages position ADzymes as highly promising candidates in the field of sensing and biosensing. This review summarizes the classification and properties of ADzymes, systematically highlighting some typical regulation strategies involving central metal, coordination environment, etc., to achieve their catalytical activity, specificity, and multifunctionality. Then, we present the recent advances of ADzymes in different sensing fields, including colorimetry, fluorescence, electrochemistry, chemiluminescence, photoelectrochemistry, and electrochemiluminescence. Taking advantage of their unique catalytic performance, the resultant ADzymes show great potential in achieving the goal of sensitivity, selectivity and accuracy for the detection of various targets. Specifically, the underlying mechanisms in terms of signal amplification were discussed in detail. Finally, the current challenges and perspectives on the development of advanced ADzymes are discussed.
σ‐π Hyperconjugation as a Design Strategy for High‐Performance Fluorescent Covalent Organic Frameworks
Authors: Liu Yin‐sheng , Xue Rui , Zhu Kai , Yan Bing 
Published: February 2025

AbstractRealizing efficient solid‐state fluorescence in covalent organic frameworks (COFs) represents a persistent and fundamental challenge in materials science, hindering their application in next‐generation optoelectronics. Herein, a transformative strategy leveraging σ‐π hyperconjugation is introduced to enhance the fluorescence properties of COFs. The incorporation of a methyl group into a pyrene‐based COF monomer yields an unprecedented fluorescence quantum yield increase of more than 100‐fold, accompanied by significantly enhanced optoelectronic performance. This enhancement arises from σ‐π hyperconjugation, which redistributes electron density, converting pyrene from an electron donor to an electron acceptor and facilitating efficient electronic transitions. Additionally, a COF‐based fluorescent acoustic sensor, fabricated via electrospinning, demonstrates real‐time speech pattern recognition when integrated with machine learning algorithms, showcasing potential in language‐assistive technologies. This study pioneers the exploration of hyperconjugation in COFs, offering a new paradigm for the precise modulation of optoelectronic properties and bridging fundamental research with real‐world applications.
Pollutant Dispersion With Multiple Release Sources Under Intermittent Ventilation Modes in Industrial Plants
Authors: Cheng Lingyan , Liu Zhan , Yin Xin 
Published: February 2025

ABSTRACTDepth understanding on the pollutant dispersion mechanism in large scale industrial buildings is significant for improving the air quality and supplying technique references on ventilation system design. Although a large amount of researches on ventilation system have been conducted, the effect of the intermittent ventilation mode on the pollutant diffusion with multiple release sources is still unclear. In the present study, the dispersion performance of hydrogen sulfide in an actual rubber processing plant was comprehensively investigated by model test and numerical simulation. The hybrid natural ventilation with mechanical exhaust was designed and used for the removal of hydrogen sulfide. A model test rig was established to measure the pollutant distribution in a transparent glass chamber with substitutive fluid sulfur hexafluoride. The numerical model of pollutant dispersion was built with considerations of temperature stratification and concentration diffusion. Compared against the test data, the prediction deviation of the numerical model was limited within 10%. The influence of the intermittent ventilation modes on the hydrogen sulfide dispersion was investigated and analyzed in detail. Some findings were obtained finally. This study could enrich the ventilation design on hydrogen sulfide dispersion in actual industrial plants and may supply some new insights on the dispersion mechanism of pollutants.
Unlocking the Secrets of Sturgeon Ecology: Lessons From Conservation Efforts in Northern Italy
Authors: Labria Fabiola , Orioli Valerio , Trasforini Stefania , Puzzi Cesare , Tirozzi Pietro , Bani Luciano , Dondina Olivia 
Published: February 2025

ABSTRACTThis study aimed to investigate the ecology of the Adriatic sturgeon (Acipenser naccarii) and Beluga sturgeon (Huso huso) in the Po basin in northern Italy, where both species have faced significant population declines. Using data collected from three LIFE projects, we investigated movement behaviour and habitat selection of the species following restocking, reintroduction and river defragmentation actions implemented during the LIFE projects. The two species showed a distinct movement behaviour and habitat selection. A. naccarii preferred to stay in pool, and it showed a tendency to have a landlocked behaviour, whereas H. huso did not show a clear habitat selection, and it always moved downstream, supporting the typical anadromous behaviour of this species. Both species benefited from river defragmentation interventions. These findings underscore the importance of habitat conservation/restoration and defragmentation measures for the long‐term survival of sturgeon species.
Reproductive cycles of the arboreal and viviparous snake Corallus hortulana (Serpentes, Boidae) from the Brazilian Amazon
Authors: Silva Karina M. P. , Pizzatto Lígia , Frazão Luciana , Almeida‐Santos Selma M. , Oliveira Maria Ermelinda 
Published: February 2025

AbstractAlthough the reproduction of some Boidae snakes has been studied through the macroscopic anatomy of the gonads, without microscopic morphology, there is little precision in describing reproductive cycles, especially for males. The relationship between the reproductive cycle—gametogenesis and reproductive seasonality throughout the year—has yet to be detailed for many Boidae species. We present macroscopic and histological data on the reproductive tracts of both male and viviparous female Corallus hortulana (Linnaeus 1758) (Amazon Tree Boa) from the Brazilian Amazon rainforest. Males present spermiogenesis and spermiation mainly during January and June (wet season to beginning‐dry season). Contrary to common assumption, we speculate that sperm production in C. hortulana, even in the tropical Amazon, is likely energetically demanding, influenced by rainfall, given that testicular involution occurred between May and November (most of the driest months of the year). Females present ovarian follicles in processes of vitellogenesis from December to August (wet season and part of the dry season). However, newborns are found in the wild mainly in the wet season, characterizing the cycle of female C. hortulana as seasonal. Unprecedentedly, we present the first microscopic description of the pouch and non‐glandular uterus in a Boidae species. The cells that compose the epithelial tissue of the non‐glandular uterus have been suggested for maintaining and supporting spermatozoa (sperm storage) in snakes, but we found no evidence that sperm storage occurs in this portion of the oviduct in C. hortulana. This study not only improves our understanding of reproductive biology in a boid, but because several aspects of reproductive cycles are conservative in the family (e.g., season of birth of newborn snakes), it aids in the development of effective conservation policies for endangered species such as Corallus cropanii, the rarest Boidae in the Americas.
Integrated Computational Approach for Designing Potent EGFR‐TK Inhibitors: QSAR, Docking, ADMET and Molecular Dynamics Studies
Authors: Patil Shital , Asgaonkar Kalyani , Bhandari Shashikant , Edake Aishwarya M. , Patel Harun , Patil Bhatu , Ansari Iqrar , Mahadik Indrani , Kamble Omkar , Gavande Navnath 
Published: February 2025

Background: The epidermal growth factor receptor (EGFR) regulates cell survival and proliferation, making it a key therapeutic target in cancer. EGFR tyrosine kinase inhibitors (TKIs) block EGFR signaling, preventing uncontrolled cell growth. However, current EGFR‐TKIs face resistance and toxicity issues, necessitating optimized pharmacophores and novel chemical entities (NCEs). Objectives: This study aimed to develop a 3D quantitative structure‐activity relationship (QSAR) model for pharmacophore optimization and to design NCEs with improved properties. Additionally, ADMET profiling, molecular docking, and molecular dynamics simulations were performed for the NCEs. Methods: Using Schrödinger’s Maestro v13.4, QSAR models were built with 42 thiazolyl‐pyrazoline derivatives, yielding a significant model (R² = 0.7880, Q² = 0.7341). Forty NCEs with favorable drug‐like properties were selected. The QikProp module assessed ADMET, while Desmond facilitated 100 ns molecular dynamics simulations. Results: NCE 2 emerged as the top candidate with a docking score of –8.178 kcal/mol, showing strong interaction with Leu 788 in the EGFR binding site. Molecular simulations confirmed the stability of the NCE 2‐EGFR complex, with RMSD values between 2.4 and 2.8 Å. Conclusion: NCE 2, a novel thiazolyl‐pyrazoline derivative, demonstrated significant EGFR inhibitory activity, stability, and favorable ADMET properties, making it a promising candidate for further development.
Estimating cancer incidence attributable to physical inactivity in the United States
Authors: Lynch Brigid M. , Bassett Julie K. , Milne Roger L. , Patel Alpa V. , Rees‐Punia Erika , Lee I‐Min , Moore Steven C. , Matthews Charles E. 
Published: February 2025

AbstractBackgroundPrevious estimates of the number of cancers attributable to physical inactivity in the United States have typically focused on only three malignancies (colon, endometrial, and postmenopausal breast cancer). Contemporary epidemiologic evidence suggests that physical inactivity could contribute to up to 15 types of cancer, and a dose–response effect has been demonstrated for 13 of these. This study estimated the number of cancers diagnosed in the United States in 2015 due to physical inactivity for these 13 sites.MethodsData from the 2005 National Health Interview Survey were used to estimate physical activity prevalence and, with the assumption of a 10‐year latency period, 2015 cancer incidence data from the National Program of Cancer Registries and Surveillance, Epidemiology, and End Results Incidence US Cancer Statistics Public Use Database.ResultsThe potential impact fraction was estimated to be 4.1%, which meant that 30,951 of 761,625 incident cancers at the 13 sites could have been prevented in the United States in 2015 if adults had increased physical activity by one category in 2005 (approximately 7.5 additional metabolic equivalent task hours per week [MET‐h/week]). Theoretically, 85,415 of 761,625 incident cancers at the 13 sites (population attributable fraction, 11.2%) could have been prevented if all adults had achieved the highest level of physical activity (>30 MET‐h/week).ConclusionsWhen estimates are based on updated epidemiologic evidence regarding physical inactivity and cancer risk, substantially more cancers are attributable to physical inactivity than previously reported. A greater focus on physical activity promotion is warranted for cancer control in the United States.
METTL14‐mediated m6A modification of ZFP14 inhibits clear cell renal cell carcinoma progression via promoting STAT3 ubiquitination
Authors: Liu Zhuonan , Sun Tianshui , Zhang Zhe , Piao Chiyuan , Kong Chuize , Zhang Xiaotong 
Published: February 2025

AbstractTherapeutic options for advanced clear cell renal cell carcinoma (ccRCC) are currently inadequate. Earlier research has shown that the enzyme methyltransferase‐like 14 (METTL14) can suppress ccRCC development through the modification of N6‐methyladenosine (m6A). This study further explored its complex biological functions and underlying molecular mechanisms. Here, we identified zinc finger protein 14 (ZFP14) as a novel target of METTL14‐mediated m6A, and its under‐expression was associated with ccRCC tumourigenesis and progression. Detailed investigations revealed that METTL14 interacted directly with the 3′ untranslated region of ZFP14 mRNA, promoting m6A modification at two specific sites. These modifications were recognised by the protein insulin‐like growth factor 2 mRNA‐binding protein 2 (IGF2BP2), which stabilised and enhanced the expression of ZFP14 mRNA. Functionally, the METTL14/ZFP14 axis suppressed in vitro growth, migration and invasiveness and in vivo proliferation and metastasis of ccRCC cells. ZFP14 potentially regulated numbers of transcripts, among which matrix metalloproteinase 1/3 (MMP1/3) were validated to be under‐expressed by ZFP14. Crucially, ZFP14 interacted with the signal transducer and activator of transcription 3 (STAT3), augmenting its K48‐linked ubiquitination and destabilising it via the proteasome pathway. Moreover, ZFP14 repressed ccRCC cell in vivo growth and metastasis as well as decreasing MMP1/3 levels by under‐expressing STAT3. These observations confirmed that ZFP14 served as both a novel target for METTL14‐mediated m6A modification and a significant tumour suppressor in ccRCC, shedding light on the cellular and molecular operations in ccRCC and opening up possibilities for novel therapeutic strategies.Key points
ZFP14 under‐expression is associated with ccRCC tumourigenesis and progression.
METTL14‐mediated m6A enhances ZFP14 mRNA stability and expression with IGF2BP2 as the reader in ccRCC.
ZFP14 promotes the degradation of STAT3 by enhancing its K48‐linked ubiquitination, inhibiting ccRCC progression.