bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of the diversity of the HIV‐1 pol gene and drug resistance associated changes among drug‐naïve patients in Burkina Faso
Authors: Tebit Denis M. , Sangaré Lassana , Tiba Fabrice , Saydou Yameogo , Makamtse Aline , Somlare Hermann , Bado Guillaume , Kouldiaty Boris G. , Zabsonre Inoussa , Yameogo Sibiri L. , Sathiandee Kanokporn , Drabo Joseph Y. , Kräusslich Hans‐Georg 
Published: August 2009

AbstractA cross‐sectional study was undertaken among drug‐naïve HIV patients at the University Hospital in Ouagadougou shortly before and after the introduction of large‐scale antiretroviral therapy (ART) in Burkina Faso. Baseline clinical and virological data as well as protease (PR) and 5′ reverse transcriptase (RT) sequences from 104 HIV infected patients were analyzed. Genotypic classification revealed the following subtypes and recombinant forms: CRF06_cpx, n = 46 (44.2%); CRF02_AG, n = 39 (37.5%); subtype A, n = 4 (3.8%); CRF09_cpx, n = 2 (1.9%); and unclassified, n = 13 (12.5%). Bootstrap analysis of CRF02_AG and CRF06_cpx viruses showed that >80% had a similar structure to their respective prototypes. The prevalence of primary drug resistance mutations was 12.5%, all mutations arising in the RT sequences in accordance with the dominance of this drug class in Burkina Faso. The mutations were distributed as follows: NRTI (10.6%): M41L (n = 2), D67N (n = 2), K70K/E (n = 2), L210W (n = 1), T215S/Y (n = 2), and K219K/Q (n = 2); NNRTI (6.1%): K103K/N (n = 2), Y181C (n = 2), G190G/A (n = 1), and P236P/L (n = 1). Subtype specific secondary polymorphisms such as K20I and M36I in the PR were observed in almost all patients. Drug resistance mutations occurred at similar frequencies (12.8% and 10.8%, respectively) among patients infected with CRF02_AG and CRF06_cpx. Some subtype specific polymorphisms were observed within important HLA epitopes, including B35, B7, and A2 in the RT, and A*6802 in the PR sequences. The observed resistance mutations are most likely to have been transmitted based on the timing of the study but prior undocumented use of ART cannot be excluded. J. Med. Virol. 81:1691–1701, 2009. © 2009 Wiley‐Liss, Inc.
Transformational and transactional leadership and innovative behavior: The moderating role of psychological empowerment
Authors: Pieterse Anne Nederveen , van Knippenberg Daan , Schippers Michaéla , Stam Daan 
Published: August 2009

AbstractInnovative behavior is increasingly important for organizations' survival. Transformational leadership, in contrast to transactional leadership, has been argued to be particularly effective in engendering follower innovative behavior. However, empirical evidence for this relationship is scarce and inconsistent. Addressing this issue, we propose that follower psychological empowerment moderates the relationship of transformational and transactional leadership with follower innovative behavior. In a field study with 230 employees of a government agency in the Netherlands combining multisource ratings, we show that transformational leadership is positively related to innovative behavior only when psychological empowerment is high, whereas transactional leadership has a negative relationship with innovative behavior only under these conditions. Copyright © 2009 John Wiley & Sons, Ltd.
Temperature reconstruction from tree‐ring maximum density of Balfour spruce in eastern Tibet, China
Authors: Wang Lily , Duan Jianping , Chen Jin , Huang Lei , Shao Xuemei 
Published: August 2009

AbstractWood from Balfour spruce [Picea likiangensis var. balfouriana (Rehd. et Wils.)] was collected at three sites in eastern Tibet. Maximum latewood densities (MXD) were measured by X‐ray densitometry; individual series were first cross dated and then combined to form a standard chronology. This chronology was significantly correlated with late summer temperatures on the eastern Tibetan Plateau. It was used to reconstruct the August–September mean temperature for the period 1695–2000 A.D., and it explained the 63.5% of the total temperature variance. Wavelet analysis of the reconstructed temperature series suggested the existence of a 20‐year climatic cycle during the period 1800–1860 and a ∼50‐year cycle at the beginning of the twentieth century. Copyright © 2009 Royal Meteorological Society
Perioperative management of patients with cytoreductive surgery for peritoneal carcinomatosis
Authors: Schmidt C. , Moritz S. , Rath S. , Grossmann E. , Wiesenack C. , Piso P. , Graf B.M. , Bucher M. 
Published: August 2009

AbstractCytoreductive surgery with hyperthermic intraperitoneal chemotherapy (HIPEC) has become an important tool in the management of patients with peritoneal malignancies. It is a complex surgical procedure with significant fluid loss during debulking leading to relevant pathophysiological alterations and therefore a challenge for anesthesiologists and critical care physicians. This review summarizes perioperative changes in hemodynamics, oxygen supply, coagulation, hematopoetic parameters and fluid status during cytoreductive surgery and HIPEC and how to deal with these pathophysiological alterations. J. Surg. Oncol. 2009;100:297–301. © 2009 Wiley‐Liss, Inc.
Evidence‐based medicine in the treatment of peritoneal carcinomatosis: Past, present, and future
Authors: Nissan Aviram , Stojadinovic Alexander , Garofalo Alfredo , Esquivel Jesus , Piso Pompiliu 
Published: August 2009

AbstractThe current treatment of peritoneal surface malignancies (PSMs) is moving from a nihilistic approach, into a combined modality approach offering selected patients long‐term survival. As primary PSM are rare, extrapolation of data from clinical trials of related disease is necessary to develop treatment guidelines. Secondary PSM are more common, and therefore, treatment guidelines should be developed based on prospective clinical trials. We reviewed the published and ongoing clinical trials studying the treatment of PSM. J. Surg. Oncol. 2009;100:335–344. © 2009 Wiley‐Liss, Inc.
Safety and efficacy of bidirectional chemotherapy for treatment of patients with peritoneal dissemination from gastric cancer: Selection for cytoreductive surgery
Authors: Yonemura Yutaka , Endou Yoshio , Shinbo Masaya , Sasaki Takuma , Hirano Masamitu , Mizumoto Akiyoshi , Matsuda Takayuki , Takao Nobuyuki , Ichinose Masumi , Mizuno Mitsukuni , Miura Masahiro , Ikeda Makoto , Ikeda Satoshi , Nakajima Gou , Yonemura Jou , Yuuba Takafumi , Masuda Seiji , Kimura Hironobu , Matsuki Nobuo 
Published: August 2009

AbstractThere is no standard treatment for peritoneal carcinomatosis (PC) from gastric cancer. New bidirectional chemotherapy (neoadjuvant intraperitoneal‐systemic chemotherapy protocol (NIPS)) was developed. The aim of the present study was to assess the safety and efficacy of NIPS and to show the selection for cytoreductive surgery on PC from gastric cancer. Seventy‐nine patients with PC from gastric cancer were treated with NIPS. A peritoneal port system was introduced into the abdominal cavity. The peritoneal wash cytological examination through a port was done before and after NIPS. The patients were treated with oral TS‐1 twice a daily for 21 days, followed by a 1‐week rest. On day 1, 8, and 15 from the start of oral TS‐1 administration, 30 mg/m2 of Docetaxel and 30 mg/m2 of cisplatinum with 500 ml of saline were introduced into the peritoneal cavity through the port. A median course of oral TS‐1 was 2.1 course and a median time of IP chemoterapy was 5.8. Peritoneal free cancer cells (PFCCs) had been detected in 65 (82.2%) patients before NIPS, and the positive cytology changed to be negative in 41 (63.0%) patients after NIPS. After NIPS, 41 patients underwent laparotomy, and complete cytoreduction was done in 32 (78%) patients. Complete cytoreduction was done in 27 (51.9%) of 52 patients with negative cytology but in only 4 (14.8%) of 27 patients with positive cytology (P < 0.001). Patients with negative cytology after NIPS survived significantly longer than those with positive cytology. The adverse effects after NIPS were mild and there was no treatment‐related deaths. The grade 3/4 hematological adverse effects were found in 2 (2.6%) patients. Grade 3 renal toxicity and port site infection was found in three patients, respectively. NIPS using a port system is a safe and effective treatment for PC. Peritoneal wash cytology through a port system is a good indicator to select the patients to perform cytoreductive surgery. J. Surg. Oncol. 2009;100:311–316. © 2009 Wiley‐Liss, Inc.
Learning curve in cytoreductive surgery and hyperthermic intraperitoneal chemotherapy
Authors: Moradi Bijan N. , Esquivel Jesus 
Published: August 2009

AbstractCytoreductive surgery and Hyperthermic Intraperitoneal Chemotherapy have achieved good long‐term results in patients with complete surgical eradication of their peritoneal dissemination but at the expense of significant perioperative morbidity and mortality. The high complication rate has been attributed to the steep learning curve associated with this procedure. We report on the current literature regarding the learning curve for this procedure and the key components that determine the success in learning this new skill. J. Surg. Oncol. 2009;100:293–296. © 2009 Wiley‐Liss, Inc.
Quality of life after cytoreductive surgery and hyperthermic intraperitoneal chemotherapy for peritoneal surface malignancies
Authors: Piso Pompiliu , Glockzin Gabriel , von Breitenbuch Phillipp , Popp Felix Cristoph , Dahlke Marc Hendrik , Schlitt Hans J. , Nissan Aviram 
Published: August 2009

AbstractCytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) is associated with high morbidity. The Quality of Life (QoL) assessment in this patient group with a limited life expectancy and high recurrence rate is important. Published data show an impairment of postoperative Quality of Life at 3 months postoperatively with an improvement over 6–12 months at levels higher than the baseline. Standardized instruments QoL have to be included in clinical trials assessing the efficacy of CRS and HIPEC. J. Surg. Oncol. 2009;100:317–320. © 2009 Wiley‐Liss, Inc.
Cytoreductive procedures—strategies to reduce postoperative morbidity and management of surgical complications with special emphasis on anastomotic leaks
Authors: Jaehne Joachim 
Published: August 2009

AbstractCytoreduction and hyperthermic intraperitoneal chemotherapy (HIPEC) for the therapy of peritoneal carcinomatosis are associated with substantial morbidity and acceptable mortality. Patient selection, learning curve, patient warming, and reduced blood loss are the main factors to decrease morbidity. Morbidity is mostly associated with bowl fistulas and anastomotic leakages. Depending on the site of leaks they may be managed conservatively or by reoperation. For standardization of study reports on morbidity and mortality the CTCAE classification is recommended. J. Surg. Oncol. 2009;100:302–305. © 2009 Wiley‐Liss, Inc.
Management of colorectal liver metastases in patients with peritoneal carcinomatosis
Authors: Izzo F. , Piccirillo M. , Palaia R. , Albino V. , Di Giacomo R. , Mastro A.A. 
Published: August 2009

AbstractWe have taken into consideration papers published in the last 10 years on the treatment of patients with peritoneal carcinomatosis and hepatic metastasis from colorectal cancer and the pre‐operative prognostic factors needed to consider these subjects eligible for surgical treatment. Peritoneal carcinomatosis should not be considered an absolute contraindication to hepatic resection if it is possible to perform a complete resection of all peritoneal and liver disease. J. Surg. Oncol. 2009;100:345–347. © 2009 Wiley‐Liss, Inc.
Predictors of peritoneal carcinomatosis in patients with gastric cancer treated at a single institution in Brazil
Authors: Fanelli Marcello F. , de Paiva Tadeu F. , Silva Milton J.B. , Benevides Carlos F.L. , Guimarães Andréa P.G. , Gimenes Daniel L. , Pinheiro Edilson D. , Rinck José A. , Nicolau Ulisses R. , Sanches Solange M. , Mello Celso A.L. , Dettino Aldo L.A. , Cruz Marcelo R.S. , de Melo Leila Maria M.P. , Formiga Maria N.C. , de Lima Vladmir C.C. , Chinen Ludmilla T.D. 
Published: August 2009

AbstractBackgroundPeritoneal carcinomatosis is a common pattern of recurrence in gastric cancer and is associated with a poor prognosis. Determining predictive factors for peritoneal recurrence can help the selection of patients suitable for more aggressive treatment strategies.MethodsA retrospective chart review of 162 patients diagnosed with gastric cancer with no peritoneal carcinomatosis and treated at a single institution in Brazil from January 1994 to December 2004 was carried out. Univariate and multivariate analyses were performed to identify patient and tumor‐related characteristics associated with the development of peritoneal metastasis.ResultsTwenty‐three (14.2%) patients developed peritoneal carcinomatosis. Three independent factors associated with the development of peritoneal metastasis were identified by multivariate analysis: signet‐ring cell histology (odds ratio [OR] = 4.9; P = 0.018), the presence of vascular invasion (OR = 4.8; P = 0.022), and the presence of visceral metastasis at diagnosis (OR = 5.1; P = 0.011). Tumor stages T3 or T4 showed a trend towards significance (P = 0.062).ConclusionsPatients with gastric cancer presenting with signet‐ring histology, vascular invasion, or visceral metastasis appear to be at higher risk for the development of peritoneal carcinomatosis. J. Surg. Oncol. 2009;100:452–455. © 2009 Wiley‐Liss, Inc.
Progression and prognosis of gastric stump cancer
Authors: Hu Xiang , Tian Da‐Yu , Cao Liang , Yi Yu 
Published: August 2009

AbstractBackground and ObjectivesThe incidence of gastric stump cancer (GSC) is increasing. The aim of this study is to determine the clinicopathologic feature and the differences of surgical outcome between GSC after partial gastrectomy for benign diseases (GSC‐B) and GSC after partial gastrectomy for malignant tumors (GSC‐M).MethodsMedical records of 42 patients with GSC‐B and 47 patients with GSC‐M who underwent surgical treatment were studied and analyzed retrospectively. Clinicopathologic parameters, the 5‐year survival rate after operation and prognostic factors, were analyzed retrospectively.ResultsGSC was frequently detected in anastomotic site. Poorly differentiated cancer was common. No difference was found between patients with GSC‐B and patients with GSC‐M in terms of histologic type, tumor location, and distribution of tumor stage. GSC‐B patients had a higher incidence in No. 7, 8, 9 lymph nodes than GSC‐M patients. In contrast, the patients with GSC‐M had higher incidence of metastasis to jejunal mesentery lymph nodes and No. 10, 11 lymph nodes. The overall 5‐year survival rates were 38.1% for GSC‐B and 10.4% for GSC‐M, with significant difference (P < 0.05).ConclusionsGSC has a particular pattern in lymph node metastasis and organs invasion. Surgical resection is considered an effective therapeutic strategy for GSC. J. Surg. Oncol. 2009;100:472–476. © 2009 Wiley‐Liss, Inc.
Electrothermal bipolar coagulation decreases the rate of red blood cell transfusions for pelvic exenterations
Authors: Bansal Nisha , Roberts William S. , Apte Sachin M. , Lancaster Johnathan M. , Wenham Robert M. 
Published: August 2009

AbstractBackgroundWe evaluated whether electrothermal bipolar coagulation (Ligasure) for pelvic exenterations decreases operative morbidity.MethodsAll cases of pelvic exenterations, for all malignancies, performed at Moffitt Cancer Center from 1999 to 2008, were identified through retrospective review of medical records. Estimated blood loss (EBL), transfusion, operative time, hospital stay, and complications were compared between Ligasure cases and non‐Ligasure cases.ResultsSeventy‐five patients underwent identified. Ligasure was used in 29/75 (39%) cases and standard techniques in 46/75 (61%) cases. Primary malignancy was gynecologic in 31/75(40%) cases (18/29, 62% Ligasure cases, 13/46, 26% non‐Ligasure cases). Patients undergoing gynecologic exenteration received fewer intraoperative packed red blood cell (PRBC) transfusions in the Ligasure group (mean = 2.8 U), compared to the non‐Ligasure group (mean = 3.8 U; P < 0.0001). The EBL was less for Ligasure cases (1,815 ml vs. 2,205 ml; P = 0.4). For all patients, Ligasure cases had lower mean transfusion (2.7 U vs. 3.2 U; P = 0.32), and EBL (1,662 ml vs. 1,843 ml; P = 0.5). The mean operating time was similar (492 min vs. 502 min). There were no cases of re‐operation for hemorrhage in either group.ConclusionsUse of electrothermal bipolar coagulation for gynecologic exenterative surgery is associated with significantly lower blood transfusions, and may decrease blood loss and transfusion in non‐gynecologic exenterative surgery. J. Surg. Oncol. 2009;100:511–514. © 2009 Wiley‐Liss, Inc.
Interaction of B7‐H1 on intrahepatic cholangiocarcinoma cells with PD‐1 on tumor‐infiltrating T cells as a mechanism of immune evasion
Authors: Ye Yufu , Zhou Lin , Xie Xiaojun , Jiang Guoping , Xie Haiyang , Zheng Shusen 
Published: August 2009

AbstractBackground and ObjectivesThe B7‐H1/PD‐1 pathway has recently been found to contribute to immune evasion of cancer cells from host immune system. This study aimed to investigate the expression of B7‐H1 and its receptor PD‐1 and to explore their significance in the progression of intraheptic cholangiocarcinoma (ICC).MethodsThirty‐one surgically resected ICC tissues and the corresponding cancer adjacent tissues were enrolled from 2006 to 2007. Immunohistochemical studies were performed with antibody of B7‐H1, PD‐1, CD8, and CD4. Apoptosis status of tumor‐infiltrating lymphocytes (TILs) was detected by TUNEL assay.ResultsExpression of B7‐H1 and PD‐1 was found to be up‐regulated in ICC tissues compared with the cancer adjacent tissues. Tumor‐related B7‐H1 expression was significantly correlated with both tumor differentiation and pTNM stage and was inversely correlated with CD8+ TILs but not CD4+ TILs. TILs in primary carcinoma showed a high level of apoptosis.ConclusionB7‐H1/PD‐1 pathway may be linked to malignant potential of ICC and contribute to tumor immune evasion by promoting CD8+ TILs apoptosis. Thus, this pathway may indeed be a potential therapeutic target in the treatment of this disease. J. Surg. Oncol. 2009;100:500–504. © 2009 Wiley‐Liss, Inc.
Surgical technique of parietal and visceral peritonectomy for peritoneal surface malignancies
Authors: Deraco Marcello , Baratti Dario , Kusamura Shigeki , Laterza Barbara , Balestra Maria Rosaria 
Published: August 2009

AbstractPeritoneal surface malignancies have been traditionally regarded as rapidly fatal conditions. Encouraging survival results has been associated to an innovative strategy involving aggressive cytoreductive surgery with total peritonectomy and heated intraperitoneal chemotherapy. Many controversial issues still remain regarding the optimization of the cytoreductive surgical procedures. The present article critically analyzes the relevant modifications from the original technique emerged during a 15‐year experience in a single center. Operative outcome results are presented. J. Surg. Oncol. 2009;100:321–328. © 2009 Wiley‐Liss, Inc.
Simultaneous determination of dihydroxybenzene and phenylenediamine positional isomers using capillary zone electrophoresis coupled with amperometric detection
Authors: Dong Shuqing , Chi Langzhu , Yang Zhiyong , He Pingang , Wang Qingjiang , Fang Yuzhi 
Published: August 2009

AbstractIn general capillary zone electrophoresis (CZE) separation models, o‐, m‐, and p‐phenylenediamine isomers can be separated in a weak acidic running buffer for their pKa values being 4.52, 5.64, 6.04, respectively, while o‐, m‐, and p‐dihydroxybenzene isomers can be separated in a weak basic buffer for their pKa values being 9.40, 9.40 and 10.04, respectively. So, it is hard to find a suitable running buffer at a fixed pH in normal CZE for simultaneous separation of these two groups of positional isomers. In this paper, a novel method based on alternately running two different pH buffers in CZE coupled with amperometric detection (CZE‐AD) was designed to simultaneously determine these two groups of positional isomers. It is found that when two different pH running buffers were employed alternately under appropriate order and time, the six analytes could be separated perfectly in less than 20 min and the detection limits were as low as 10–7 mol/L. Furthermore, the effects of working electrode potential, pH and concentration of running buffer, separation voltage and injection time on CZE–AD were investigated. Experimental results demonstrated that the introduced method was practical to simultaneously determine two groups of positional isomers with different pKa and had some advantages of high sensitivity, good repeatability and small sample requirement.
Simultaneous densitometric determination of shikonin, acetylshikonin, and β‐acetoxyisovaleryl‐shikonin in ultrasonic‐assisted extracts of four Arnebia species using reversed‐phase thin layer chromatography
Authors: Sharma Nandini , Sharma Upendra K. , Gupta Ajai P. , Devla  , Sinha Arun K. , Lal Brij , Ahuja Paramvir S. 
Published: August 2009

AbstractA simple, precise, and rapid high‐performance thin‐layer chromatographic (HPTLC) method for the simultaneous quantification of pharmacologically important naphthoquinone shikonin (1) together with its derivatives acetylshikonin (2), and β‐acetoxyisovalerylshikonin (3) in four species of genus Arnebia (A. euchroma, A. guttata, A. benthamii, and A. hispidissima) from the Indian subcontinent has been developed. In addition, the effect of solvents with varying polarity (hexane, chloroform, ethyl acetate, and methanol) for the extraction of these compounds was studied. HPTLC was performed on precoated RP‐18 F254S TLC plates. For achieving good separation, mobile phase consisting of ACN/methanol/5% formic acid in water (40:02:08 v/v/v) was used. The densitometric determination of shikonin derivatives was carried out at 520 nm in reflection/absorption mode. The method was validated in terms of linearity, accuracy, precision, robustness, and specificity. The calibration curves were linear in the range of 100–600 ng for shikonin and acetylshikonin, and 100–1800 ng for β‐acetoxyisovalerylshikonin. Lower LOD obtained for compounds 1–3 were 18, 15, and 12 ng, respectively, while the LOQ obtained were 60, 45, and 40 ng, respectively.
Identification of volatile compounds of Atractylode lancea Rhizoma using supercritical fluid extraction and GC–MS
Authors: Chen Qinhua , Li Peng , Yang Handong , Li Xiulou , Zhu Jun , Chen Fuchao 
Published: August 2009

AbstractSupercritical fluid was used to extract volatile components from the rhizoma of Atractylode lancea (A. lancea). An orthogonal array design (OAD), L9 (3)4, was employed as a chemometric method for the optimization of the supercritical fluid extraction (SFE) of volatile compounds from the herbal medicine. Four parameters, namely, pressure, temperature, dynamic extraction time, and flow rate of CO2, were studied and optimized by a three‐level OAD in which the interactions between the parameters were neglected. These compounds were identified according to their retention times and mass spectra by GC–MS. A total of 30 compounds of SFE extracts were identified. Atractylon (8.63%), hinesol (1.44%), β‐eudesmol (6.64%), elemol (0.42%), and atractydin (13.92%) were the major sesquiterpenes identified in A. lancea SFE extracts.