bluuebunny/crossref_metadata_2025_split huggingface dataset:
Misspellings or “miscellings”—Non‐verifiable and unknown cell lines in cancer research publications
Authors: Oste Danielle J. , Pathmendra Pranujan , Richardson Reese A. K. , Johnson Gracen , Ao Yida , Arya Maya D. , Enochs Naomi R. , Hussein Muhammed , Kang Jinghan , Lee Aaron , Danon Jonathan J. , Cabanac Guillaume , Labbé Cyril , Davis Amanda Capes , Stoeger Thomas , Byrne Jennifer A. 
Published: May 2024

AbstractReproducible laboratory research relies on correctly identified reagents. We have previously described gene research papers with wrongly identified nucleotide sequence(s), including papers studying miR‐145. Manually verifying reagent identities in 36 recent miR‐145 papers found that 56% and 17% of papers described misidentified nucleotide sequences and cell lines, respectively. We also found 5 cell line identifiers in miR‐145 papers with misidentified nucleotide sequences and cell lines, and 18 cell line identifiers published elsewhere, that did not represent indexed human cell lines. These 23 identifiers were described as non‐verifiable (NV), as their identities were unclear. Studying 420 papers that mentioned 8 NV identifier(s) found 235 papers (56%) that referred to 7 identifiers (BGC‐803, BSG‐803, BSG‐823, GSE‐1, HGC‐7901, HGC‐803, and MGC‐823) as independent cell lines. We could not find any publications describing how these cell lines were established. Six cell lines were sourced from cell line repositories with externally accessible online catalogs, but these cell lines were not indexed as claimed. Some papers also stated that short tandem repeat (STR) profiles had been generated for three cell lines, yet no STR profiles could be identified. In summary, as NV cell lines represent new challenges to research integrity and reproducibility, further investigations are required to clarify their status and identities.
Gynecologic oncology robot‐assisted surgery in octogenarians: Impact of age on hospital stay
Authors: Levin Gabriel , Brezinov Yoav , Brodeur Melica Nourmoussavi , Salvador Shannon , Lau Susie , Gotlieb Walter 
Published: May 2024

AbstractObjectiveTo compare postoperative stay in octogenarians and younger patients undergoing gynecologic oncology robot‐assisted surgery.MethodsA retrospective review of robot‐assisted surgery in Gynecological Oncology division during 2019–2022. We included all consecutive cases. Octogenarians (age ≥80 years) and younger patients were investigated by univariable analysis for characteristics and outcome.ResultsA total of 816 robot‐assisted surgeries were performed, 426 (52.2%) endometrial cancer, 159 (19.5%) ovarian cancer, 27 (3.3%) cervical cancer, 35 (4.3%) endometrial intraepithelial neoplasia, and in 169 (20.7%) the final pathology was benign. There were 60 (7.4%) octogenarians and 756 (92.6%) younger patients. The proportion of patients with an American Society of Anesthesiology score greater than 2 was higher among octogenarians (66.7% vs 32.0%, P < 0.001). The median console time, surgical time, and total operation theater time were similar between groups (P = 0.303, P = 0.643 and P = 0.688, respectively). Conversion rate did not differ between groups (0.4% among younger patients vs 0% in octogenarians, P > 0.99). The median length of stay in the recovery room was similar in both groups (median 170 min, interquartile range [IQR] 125–225 min vs 170 min, IQR 128–240 min in octogenarians, P = 0.731). Length of hospital stay was similar in both age groups; median 1 day (IQR 1–1) among octogenarians versus 1 (0–1) in younger patients (P = 0.136).ConclusionOctogenarians undergoing robotic surgery have no increased risk of length of stay or conversion to laparotomy compared with younger patients.
Silver sulphide nanoparticles (Ag2SNPs) synthesized using Phyllanthus emblica fruit extract for enhanced antibacterial and antioxidant properties
Authors: Renuka R. , Thilagavathi T. , Inmozhi C. , Uthrakumar R. , Gobi G. , Kaviyarasu K. , Al‐Sowayan Noorah Saleh , Mir Tanveer Ahmad , Alam Mir Waqas 
Published: May 2024

AbstractIn this study, silver sulfide nanoparticles (Ag2SNP's) were successfully produced by using fruit extracts of Phyllanthus emblica. UV–vis, FTIR, XRD with SEM and EDX techniques were used for the synthesis process and for characterization of the resulting nanostructures. According to the findings, the fabricated nanostructure had a monoclinic crystal structure, measuring 44 nm in grain size, and its strain was 1.82 × 10−3. As revealed by SEM analysis, the synthesized nanostructure consists of irregular spherical and triangular shapes. The presence of silver (Ag) and sulfur (S) was also confirmed through EDX spectra. Furthermore, Ag2S nanoparticles were tested for their ability to effectively inhibit gram‐positive and gram‐negative bacterial growth. As a result of this study, it was clearly demonstrated that Ag2S nanoparticles possess powerful antibacterial properties, particularly when it came to inhibiting Escherichia coli growth. Ag2S nanoparticles had high total H2O2 and flavonoid concentrations and the greatest overall antioxidant activity, according to the evaluation of antioxidant activity of the samples. The results obtained from the P. emblica fruit extract were followed by those obtained from Ag2S nanoparticles were reported in detail.Research Highlights
Innovative Ag2SNP synthesis using Phyllanthus emblica fruit extract.
SEM with EDX revealed a monoclinic crystal structure with a grain size of 44 nm and a strain of 1.82 × 10−3.
Many of these applications are demonstrated by the potential of Ag2SNPs to treat and combat bacteria, particularly Escherichia coli.
A peak at 653 cm−1 indicates the presence of primary sulfide aliphatic C‐S extension vibrations.
The abundant H2O2 and NO2 found in P. emblica nanocomposites make them potent antioxidants.
Critical reappraisal of prognostic indicators for 949 mucinous appendiceal neoplasm patients
Authors: Sugarbaker Paul H. , Chang David 
Published: May 2024

AbstractBackground and ObjectivesThe standard of care for treatment of an appendiceal mucinous neoplasm with peritoneal dissemination is cytoreductive surgery (CRS) combined with hyperthermic intraperitoneal chemotherapy (HIPEC). These two treatments are combined in the operating room. A crucial requirement for benefit long‐term is proper patient selection. Clinical and histopathologic prognostic indicators are used, along with the patient's fitness for surgery, to select patients to receive CRS and HIPEC.MethodsThis study seeks to identify the reliable prognostic indicators for four different groups of patients. They are (1) the low‐grade appendiceal mucinous neoplasms (LAMN) with a complete CRS, (2) the mucinous appendiceal adenocarcinomas (MACA) with complete CRS, (3) MACA with lymph node metastases (MACA‐LN) with complete CRS, and (4) all histologic subtypes with incomplete cytoreduction. The prognostic indicators were evaluated for their impact on overall survival in these four groups of patients.ResultsThe completeness of cytoreduction (CC) score statistically significantly showed survival differences in all three histologic subtypes. The peritoneal cancer index (PCI) showed significance with LAMN and MACA‐LN but not with MACA and not with incomplete CRS. The prior surgical score (PSS) was a prognostic indicator that predicted the outcome with LAMN, MACA‐LN, and incomplete CRS patients but not with the MACA group. Patients who were symptomatic or who had extensive systemic chemotherapy before CRS had a significantly reduced survival.ConclusionThe utility of prognostic indicators varied greatly within our four different groups of appendiceal mucinous neoplasms. CC score was always a reliable prognosticator. Surprisingly, PCI was not.
Patient‐ and system‐level factors associated with racial/ethnic disparities in the delivery of guideline‐concordant therapy among US patients with gastric cancer
Authors: da Costa Wilson L. , Tan Mimi C. , Camp E. Ramsay , Thrift Aaron P. 
Published: May 2024

AbstractBackgroundDisparities in gastric cancer (GC) outcomes show a higher disease burden among minorities. We aimed to evaluate the associations between sociodemographic and system‐level factors and guideline‐concordant treatment among GC patients.MethodsCohort study with GC patients in the National Cancer Data Base (2006–2018) treated with upfront resection or neoadjuvant therapy (NAT). We used logistic regression to identify associations between deviations from guideline‐concordant therapy and patient‐ and system‐level factors, and Cox regression models to assess risk of death.ResultsThe cohort included 43 597 GC patients treated with endoscopic resection (8.9%), surgery only (47.1%), surgery and adjuvant therapy (20.6%), or NAT followed by surgery (23.5%). A total of 31 470 patients (72.2%) received guideline‐concordant therapy. Relative to Non‐Hispanic Whites (NHWs), Non‐Hispanic Blacks (NHBs) (odds ratio [OR] 1.19, [95% confidence intervals 1.10–1.28]) and Asian/Pacific Islanders (APIs) (OR 1.12 [1.03–1.23]) had an increased risk of deviations from treatment guidelines. Medicare/Medicaid increased the risk of deviations while treatment at high‐volume facilities decreased its risk for all races/ethnicities. Deviations from guidelines were associated with an increased risk of death (hazard ratio 1.56 [1.50–1.63].ConclusionsRacial disparities in the delivery of guideline‐concordant therapy among GC patients are affected by several sociodemographic factors at the patient‐ and system‐level.
More than 3 mm of preoperative medial meniscal extrusion is identified as a key risk factor for varus progression in limb alignment after arthroscopic repair of medial meniscus posterior root tear
Authors: Yoon Kyoung Ho , Bae Bo Seung , Ahn Jun Hyeong , Hwang Sung Hyun 
Published: May 2024

AbstractPurposeTo investigate the risk factors for varus progression after arthroscopic medial meniscal posterior root tear (MMPRT) repair and to compare the clinical outcomes between two groups: one with more varus progression and the other with less varus progression.MethodsPatients who underwent isolated arthroscopic repair of MMPRT between 2015 and 2020 were enroled, and 2‐year follow‐up data were collected. Participants were categorized into two groups based on preoperative values of the weight‐bearing line (WBL) ratio: group A with <5.9% increase and group B with ≥5.9% increase. Various factors, including demographic features and radiological findings, were analysed and compared between the two groups. Intra‐meniscal signal intensity, meniscal healing, medial meniscal extrusion (MME), and articular cartilage grade were assessed preoperatively and 1‐year postoperatively using coronal magnetic resonance imaging.ResultsThe final cohort consisted of 34 patients in group A and 46 in group B, with a mean age of 55.8 ± 11.2 and 59.8 ± 6.6 years, respectively. Preoperative WBL ratio and cartilage lesions in the medial compartment did not differ between the groups. Preoperative MME were significantly lower in group A than those in group B (2.6 ± 0.6 mm in group A and 3.5 ± 0.7 mm in group B, p < 0.05). Patient‐reported outcomes at the 2‐year follow‐up did not differ between the two groups (n. s.). In a logistic analysis, the odds ratio of MME was 2.1 (p < 0.05), and the cutoff value of MME was 3.02 mm.ConclusionPreoperative MME is a risk factor for varus progression. However, no differences in patient‐reported outcomes were observed at 2‐year follow‐up, even in the group with greater varus progression.Level of EvidenceLevel IV.
Surgical treatment for recurrent patellar dislocation with severe torsional deformities: Double‐level derotational osteotomy may not have a clear advantage over single‐level derotational osteotomy in improving clinical and radiological outcomes
Authors: Zhang Zhijun , Wang Daofeng , Di Menglinqian , Zheng Tong , Zhang Hui 
Published: May 2024

AbstractPurposeThe purpose of this study was to investigate whether double‐level (femur + tibia) derotational osteotomy is superior to single‐level femoral derotational osteotomy for recurrent patellar dislocation with severe femoral and tibial rotational deformities (femoral anteversion >30° and external tibial torsion >30°).MethodsBetween January 2015 and June 2020, a total of 115 knees with recurrent patellar dislocation treated with combined medial patellofemoral ligament reconstruction (MPFL‐R) and derotational osteotomies were evaluated after a minimum follow‐up of 2 years. Among these cases, 15 knees that underwent double‐level derotational osteotomy were included in the double‐level group, which was propensity‐matched in a 1:2 ratio to a single‐level group of patients who underwent single‐level femoral derotational osteotomy (30 knees). The clinical and radiological outcomes were evaluated and compared between the groups. Furthermore, the foot progression angle was measured preoperatively and 2 years after surgery.ResultsThe patient‐specific variables did not differ significantly between the double‐ and the single‐level groups after propensity score matching. The postoperative mean foot progression angle was significantly lower in the double‐level group than in the single‐level group (9° ± 8° vs. 15° ± 11°; p = 0.014); however, there were no statistically significant differences between the groups in terms of any other clinical and radiological assessments.ConclusionFor patients with severe femoral and tibial torsional deformities (femoral anteversion >30° and external tibial torsion >30°), the double‐level derotational osteotomy is superior to single‐level osteotomy in maintaining normal foot progression angle, but it does not show an advantage in terms of patient‐reported outcomes, radiological results and redislocation rate at minimum 2 years of follow‐up. Furthermore, concomitant excessive external tibial torsion (>30°) did not have an adverse effect on clinical outcomes in patients who underwent derotational distal femoral osteotomy with MPFL‐R due to excessive femoral anteversion.Level of EvidenceLevel III.
M6A‐mediated hsa_circ_0061179 inhibits DNA damage in ovarian cancer cells via miR‐143‐3p/TIMELESS
Authors: Zhang Yuhong , Wu Yuhong , Shi Xiu , Ding Hongmei , Zhou Ying , Chen Hanqing , Shen Fangrong , Chen Youguo , Zhou Jinhua , Zhou Dingjie , Wang Juan 
Published: May 2024

AbstractOvarian cancer (OC) is among the most common and deadly solid malignancies in women. Despite many advances in OC research, the incidence of OC continues to rise, and its pathogenesis remains largely unknown. Herein, we elucidated the function of hsa_circ_0061179 in OC. The levels of hsa_circ_0061179, miR‐143‐3p, TIMELESS, and DNA damage repair‐related proteins in OC or normal ovarian tissues and cells were measured using real‐time quantitative polymerase chain reaction and immunoblotting. The biological effects of hsa_circ_0061179 and miR‐143‐3p on proliferation, clone formation, DNA damage, and apoptosis of OC cells were detected by the cell counting kit‐8 assay, 5‐methylethyl‐2′‐deoxyuridine, flow cytometry, the comet assay, and immunofluorescence staining combined with the confocal microscopy. The interaction among hsa_circ_0061179, miR‐143‐3p, and TIMELESS was validated by the luciferase reporter assay. Mice tumor xenograft models were used to evaluate the influence of hsa_circ_0061179 on OC growth in vivo. We found that human OC biospecimens expressed higher levels of hsa_circ_0061179 and lower levels of miR‐143‐3p. Hsa_circ_0061179 was found to bind with miR‐143‐3p, which directly targets TIMELESS. Hsa_circ_0061179 knockdown or miR‐143‐3p overexpression suppressed the proliferation and clone formation of OC cells and increased DNA damage and apoptosis of OC cells via the miR‐143‐3p/TIMELESS axis. Furthermore, we demonstrated that METTL3 could direct the formation of has_circ_0061179 through a specific m6A modification site. YTHDC1 facilitated the cytoplasmic transfer of has_circ_0061179 by directly binding to the modified m6A site. Our findings suggest that hsa_circ_0061179 acts as the sponge of miR‐143‐3p to activate TIMELESS signaling and inhibits DNA damage and apoptosis in OC cells.
Simultaneous analysis of continuously embedded Reissner–Mindlin shells in 3D bulk domains
Authors: Kaiser Michael Wolfgang , Fries Thomas‐Peter 
Published: May 2024

AbstractA mechanical model and numerical method for the simultaneous analysis of Reissner–Mindlin shells with geometries implied by a continuous set of level sets (isosurfaces) over some three‐dimensional bulk domain is presented. A three‐dimensional mesh in the bulk domain is used in a tailored FEM formulation where the elements are by no means conforming to the level sets representing the shape of the individual shells. However, the shell geometries are bounded by the intersection curves of the level sets with the boundary of the bulk domain so that the boundaries are meshed conformingly. This results in a method which was coined Bulk Trace FEM before. The simultaneously considered, continuously embedded shells may be useful in the structural design process or for the continuous reinforcement of bulk domains. Numerical results confirm higher‐order convergence rates.
The Use of Artificial Intelligence to Improve Readability of Otolaryngology Patient Education Materials
Authors: Patel Evan A. , Fleischer Lindsay , Filip Peter , Eggerstedt Michael , Hutz Michael , Michaelides Elias , Batra Pete S. , Tajudeen Bobby A. 
Published: May 2024

AbstractObjectiveThe recommended readability of health education materials is at the sixth‐grade level. Artificial intelligence (AI) large language models such as the newly released ChatGPT4 might facilitate the conversion of patient‐education materials at scale. We sought to ascertain whether online otolaryngology education materials meet recommended reading levels and whether ChatGPT4 could rewrite these materials to the sixth‐grade level. We also wished to ensure that converted materials were accurate and retained sufficient content.MethodsSeventy‐one articles from patient educational materials published online by the American Academy of Otolaryngology–Head and Neck Surgery were selected. Articles were entered into ChatGPT4 with the prompt “translate this text to a sixth‐grade reading level.” Flesch Reading Ease Score (FRES) and Flesch‐Kincaid Grade Level (FKGL) were determined for each article before and after AI conversion. Each article and conversion were reviewed for factual inaccuracies, and each conversion was reviewed for content retention.ResultsThe 71 articles had an initial average FKGL of 11.03 and FRES of 46.79. After conversion by ChatGPT4, the average FKGL across all articles was 5.80 and FRES was 77.27. Converted materials provided enough detail for patient education with no factual errors.DiscussionWe found that ChatGPT4 improved the reading accessibility of otolaryngology online patient education materials to recommended levels quickly and effectively.Implications for PracticePhysicians can determine whether their patient education materials exceed current recommended reading levels by using widely available measurement tools, and then apply AI dialogue platforms to modify materials to more accessible levels as needed.Level of EvidenceLevel 5.
An Improved Staging System of Adenoid Cystic Carcinoma in the External Auditory Canal
Authors: Wang Jing , Li Feitian , Feng Yisi , Kong Dedi , Zhang Yibo , Dai Chunfu 
Published: May 2024

AbstractObjectiveThe purpose of this study was to define an improved staging system for adenoid cystic carcinoma (ACC) in the external auditory canal (EAC) based on biological behaviors, image findings, and the prognosis of patients with ACC in the EAC.Study DesignA retrospective study.SettingA single center data.MethodsWe performed a single‐institution retrospective review of 154 patients with ACC in the EAC between January 2004 and September 2021. Risk factors associated with disease‐free survival (DFS) and cancer‐specific survival (CSS) of ACC in the EAC were identified using univariate and multivariate cox regression analysis. Then an improved staging system was proposed and compared with the Pittsburgh‐modified tumor, node, and metastasis (TNM) staging system for statistical differences in DFS and CSS.ResultsAn improved staging system of ACC in the EAC was defined, in which stage T4 were subclassified into T4a and T4b and were statistically different from the Pittsburgh‐modified TNM staging system in DFS and CSS. We also found that the dura mater, facial nerve, sigmoid sinus, deep lobe of parotid gland, and parapharyngeal space involvement were significantly associated with poor prognosis of ACC in the EAC.ConclusionThe improved staging system is more accurate in predicting survival prognosis than Pittsburgh‐modified TNM staging system for patients with ACC in the EAC, and may provide more efficient guidance of treatment strategy.SummaryThe improved staging system of ACC in the EAC is more accurately to predict survival prognosis, and provide guidance of treatment plan than Pittsburgh‐modified TNM staging system.Level of Evidence4.
2,3‐Epoxyamide‐alcohols in Domino Reactions: En Route to Molecular Diversity
Authors: El Bouakher Abderrahman , Lhoste Jérôme , Martel Arnaud , Comesse Sébastien 
Published: May 2024

AbstractThe synthesis of polycyclic γ‐ and δ‐lactams bearing up to four contiguous fully controlled stereocenters is presented. For that purpose, we developed an original approach based on the use of 2,3‐epoxyamides in domino reactions by taking advantage of the nucleophilic nitrogen atom and electrophilic epoxide. In reaction with enol ethers bearing gem bis‐electrophiles on the double bond as Michael acceptors, four different reaction pathways were observed. They all started with a domino oxa‐Michael/aza‐Michael/epoxide opening sequence and depending on substrates engaged could be followed either by a lactonization or a hemiketalization/retro‐aldol cascade. Thus, four original fully‐substituted piperidine‐ or pyrrolidine‐2‐one scaffolds were selectively synthesized in good to high yields. Moreover, these polycyclic lactams were obtained in high stereo‐ and chemo‐selectively highlighting the efficiency and molecular diversity offered by this new methodology that should offer various synthetic opportunities in the future.