bluuebunny/crossref_metadata_2025_split huggingface dataset:
Occurrence of pathogenicYersinia enterocoliticaandYersinia pseudotuberculosisin small wild rodents
Authors: BACKHANS A. , FELLSTRÖM C. , LAMBERTZ S. THISTED 
Published: November 2010

SUMMARYRodents are a potential source of pathogenicYersinia enterocoliticaandY. pseudotuberculosis. In order to study this, 190 rodents were captured and sampled on seven pig farms (n=110), five chicken farms (n=55) and six other locations (n=25) in Sweden. Pigs from three of the pig farms were also sampled (n=60). PathogenicY. enterocoliticawas detected by TaqMan PCR in about 5% of rodent samples and 18% of pig samples. Only rodents caught on pig farms tested positive for the pathogen.Y. enterocoliticabioserotype 4/O:3 strains isolated from the rodent and pig samples were compared by pulsed-field gel electrophoresis and revealed a high degree of similarity, which was confirmed by random amplified polymorphic DNA.Y. pseudotuberculosiswas only detected in one rodent sample. Thus, rodents may be vectors for the transmission of pathogenicY. enterocoliticato pigs, acting as carriers rather than a reservoir, and should therefore remain an important issue in hygiene control measures on farms.
Observational study of the epidemiology and outcomes of vancomycin-resistantEnterococcusbacteraemia treated with newer antimicrobial agents
Authors: McKINNELL J. A. , PATEL M. , SHIRLEY R. M. , KUNZ D. F. , MOSER S. A. , BADDLEY J. W. 
Published: November 2010

SUMMARYVancomycin-resistantEnterococcusbloodstream infections (VRE-BSI) are a growing problem with few clinical trials to guide therapy. We conducted a retrospective study of management and predictors of mortality for VRE-BSI at a tertiary-care centre from January 2005 to August 2008. Univariate and multivariable analyses examined the relationship of patient characteristics and antibiotic therapy with 30-day all-cause mortality. Rates of VRE-BSI increased from 0·06 to 0·17 infections/1000 patient-days (P=0·03). For 235 patients, 30-day mortality was 34·9%. Patients were primarily treated with linezolid (44·2%) or daptomycin (36·5%). Factors associated with mortality were haemodialysis [odds ratio (OR) 3·2, 95% confidence interval (CI) 1·6–6·3,P=0·007], mechanical ventilation (OR 3·7, 95% CI 1·3–10·4,P=0·01), and malnutrition (OR 2·0, 95% CI 1·0–4·0,P=0·046). Use of linezolid, but not daptomycin (P=0·052) showed a trend towards an association with survival. In conclusion, VRE-BSI is a growing problem, associated with significant 30-day mortality. Multiple factors were associated with poor outcomes at our hospital.
Prevalence of Coxiella burnetii (Q fever) antibodies in bovine serum and bulk-milk samples
Authors: RYAN E. D. , KIRBY M. , COLLINS D. M. , SAYERS R. , MEE J. F. , CLEGG T. 
Published: November 2010

SUMMARYQ fever (Coxiella burnetii) is a zoonotic disease of increasing public health importance. The objective of this study was to estimate the prevalence of, and risk factors associated with, exposure to C. burnetii in cattle in the Republic of Ireland. Bulk-tank milk samples from 290 dairy herds and 1659 sera from 332 dairy and beef herds, randomly sampled, were tested by indirect ELISA to detect antibodies to C. burnetii. In total, 37·9% of bulk-milk sample herds and 1·8% of sera (from 6·9% of herds) were antibody positive. Of risk factors tested using logistic regression analysis, only large herd size (bulk-milk analysis) and dairy breed (serum analysis) significantly increased the odds of being positive for antibodies to C. burnetii. Herds with positive milk or serum samples were randomly distributed throughout the Republic of Ireland and no clustering was observed. The use of an ELISA to test bulk-milk samples collected by randomized stratified sampling is a cost-effective method by which national herd prevalence can be estimated by active surveillance.
Chikungunya epidemic-related mortality
Authors: MANIMUNDA S. P. , MAVALANKAR D. , BANDYOPADHYAY T. , SUGUNAN A. P. 
Published: November 2010

SUMMARYPort Blair, the capital city of the Union Territory of Andaman and Nicobar Islands in the republic of India, witnessed an outbreak of chikungunya (CHIK) fever in 2006. Although no deaths attributable to CHIK fever were registered, thousands of people were affected. In view of evidence from other parts of the world indicating that CHIK fever does cause death we studied the mortality trend in Port Blair from 2002 to 2008 in order to verify if there was increased mortality during the CHIK fever epidemic. The expected number of monthly deaths in 2006 was calculated by multiplying the average monthly mortality rate from 2002 to 2008 (with the exception of 2006) with the monthly population in 2006. The results indicated that there was a significant increase in expected deaths during some months of 2006, which coincided with the peak in the CHIK fever epidemic in Port Blair.
Domestic space and identity: artisans, shopkeepers and traders in sixteenth-century Siena
Authors: HOHTI PAULA 
Published: November 2010

ABSTRACT:Historians of early modern Italy have traditionally viewed the city's public spaces, such as streets, quarters, taverns and marketplaces, as the chief locations in which claims to identity were launched into the broader urban community. Recent studies on the domestic interior, however, have shown that the distinction between ‘public’ and ‘private’ in the fifteenth- and sixteenth-century urban space was much more complex. In this period, private urban houses became sites for an increasing range of social acitvities that varied from informal evening gatherings to large wedding banquets. Focusing on this ‘public’ dimension of the private urban house, this article explores how the middling classes of artisans and shopkeepers used the domestic space to construct identities and to facilitate social relations in sixteenth-century Siena. The aim is to show that in providing a setting for differing forms of economic and social activity, the urban home together with its objects and furnishings may have provided an increasingly important physical location for craftsmen, shop-owners and traders to negotaite individual and collective identities within the broader communities of the city.
Between the king and the pope: French cardinals in Rome (1495–1560)
Authors: BARDATI FLAMINIA 
Published: November 2010

ABSTRACT:On account of their dual function as princes of the church and agents for the king of France, the French cardinals in Rome constitute a well-defined and self-contained community. They were governed by complex internal dynamics as well as by the need to present a unified front to the pope, in addition to the College of Cardinals and the citizenry of Rome. French cardinals present in Rome between 1490 and 1560 were mobile, as their physical presence in the city was not continuous: a number of them were stable residents in Rome, charged with diplomatic missions, while others only attended the conclaves. A special case is that of Jean du Bellay, who became fully integrated into the life of the city, established a literary salon open to artists and poets, and was involved in the study of Antiquities and the construction of a villa-garden complex, theHorti Bellayani.
The via Papalis in early cinquecento Rome: a contested space between Roman families and curials
Authors: CAFÀ VALERIA 
Published: November 2010

ABSTRACT:On the definitive return of the pope to Rome in 1420, following the so-called Avignonese captivity, the city underwent major modifications. The ‘romanam curiam sequentes’, the court and administration that followed the traditionally itinerant pope, settled in the city, leading to Rome's population doubling in the space of a few years. Furthermore, with the support of the pope, the members of the curia came to take possession of spaces, offices, roles and rituals that had previously been the reserve of the local Romans. This article considers the reaction of the community of the local nobility (here described summarily as Roman families) to the encroaching presence of the curia within the specific context of the development of the built form of the via Papalis. It is argued that the via Papalis, one of the most important and prestigious streets in Rome, became the theatre within which these two communities played out their conflict through the medium of built and ephemeral architecture.
Courtiers and musicians meet in the streets: the Florentine mascherata under Cosimo I
Authors: CANGUILHEM PHILIPPE 
Published: November 2010

ABSTRACT:It is well known that the shift from canti carnascialeschi to mascherate, which took place in Florence during the first half of the sixteenth century, corresponds to parallel political changes from republic to duchy. The role of the new Duke Cosimo I de’ Medici in the transformation of Florentine street festivals must be appraised through his aim of establishing a courtly society in which the nobility were subjected to his control. In this article, this double process of change is observed through documents which show how the courtly mascherata can be viewed as a means by which courtiers sought to be identified as a homogeneous group by all Florentine citizens, looking at and listening to them from their windows.
Attending to Translocal Identities: How Congolese Anglicans Talk about their Church
Authors: Wild-Wood Emma 
Published: November 2010

AbstractIn the perennial discussions about Anglican identity some voices predominate more than others.L’Eglise Anglicane du Congois a small province with a modest voice in the Anglican Communion. This article looks at Congolese Anglican identity as articulated by its members and examines the way in which the formation of that identity emerges from local concerns as well as wider networks. It uses interviews with members to focus on the majority appreciation of ‘order’, as expressed in governance and ritual, and recent shifts in the discourses surrounding ‘order’ to engage with changes in the country. The article borrows the terms ‘translocal’ and ‘transnational’ from the social sciences to explore the overlapping relational identities that emerge and the multi-directional dynamics of Congolese Anglicans. It suggests that this approach may have wider implications for understanding Anglicanism.
MaizeGDB , maize genetics cooperation and the ~2500MB B73 genome generated tsunami
Authors: Schaeffer Mary , Gardiner Jack , Harper Elisabeth , Andorf Carson , Campbell Darwin , Cannon Ethalinda , Sen Taner , Lawrence Carolyn 
Published: November 2010

AbstractAdvances in sequencing technology have made it possible to sequence the 2500 MB B73 maize genome, both cheaply and in a relatively short time. Nearly simultaneously, other sequencing-based data are on the leading edge of a data tsunami: sequenced differences (currently >300,000 SNP for >1000 inbred lines and related species) and gene expression data. The MaizeGDB team integrates access to these datasets after considerable cooperation among both data users and suppliers, and typically while the data are being generated. In this way, data suppliers have resources in place to format data ready for integration with MaizeGDB. And, we learn what details, including links to other resources, are needed to support our user community and what interface development is required. An interesting result is that we inadvertently serve as outreach for the data suppliers, explaining the caveats and expected availabilities of data not yet at MaizeGDB. Contact with users is at conferences, by online tutorials, personal visits, or by surveys from the Maize Executive Genetics Committee addressed to all persons listed in MaizeGDB as a Cooperator.
Conditional expression of the FTO gene product in rat INS-1 cells reveals its rapid turnover and a role in the profile of glucose-induced insulin secretion
Authors: Russell Mark A. , Morgan Noel G. 
Published: November 2010

Common polymorphisms within the FTO (fat mass and obesity-associated) gene correlate with increased BMI (body mass index) and a rising risk of Type 2 diabetes. FTO is highly expressed in the brain but has also been detected in peripheral tissues, including the endocrine pancreas, although its function there is unclear. The aim of the present study was to investigate the role of FTO protein in pancreatic β-cells using a conditional expression system developed in INS-1 cells. INS-1 cells were stably transfected with FTO–HA (haemagluttinin) incorporated under the control of a tetracycline-inducible promoter. Induction of FTO protein resulted in localization of the tagged protein to the nucleus. The level of FTO–HA protein achieved in transfected cells was tightly regulated, and experiments with selective inhibitors revealed that FTO–HA is rapidly degraded via the ubiquitin/proteasome pathway. The nuclear localization was not altered by proteasome inhibitors, although following treatment with PYR-41, an inhibitor of ubiquitination, some of the protein adopted a perinuclear localization. Unexpectedly, modestly increased expression of FTO–HA selectively enhanced the first phase of insulin secretion when INS-1 monolayers or pseudoislets were stimulated with 20 mM glucose, whereas the second phase remained unchanged. The mechanism responsible for the potentiation of glucose-induced insulin secretion is unclear; however, further experiments revealed that it did not involve an increase in insulin biosynthesis or any changes in STAT3 (signal transducer and activator of transcription 3) expression. Taken together, these results suggest that the FTO protein may play a hitherto unrecognized role in the control of first-phase insulin secretion in pancreatic β-cells.
First Report of Pepino mosaic virus Infecting Greenhouse Cherry Tomatoes in Greece
Authors: Efthimiou K. E. , Gatsios A. P. , Aretakis K. C. , Papayiannis L. C. , Katis N. I. 
Published: November 2010

Pepino mosaic virus (PepMV) (genus Potexvirus, family Flexiviridae) is a mechanically transmitted virus that has emerged as a significant problem of greenhouse tomato crops in Europe and around the world during the past 10 years (1). In spring of 2010, mosaic symptoms were observed on leaves of cherry tomato (Lycopersicon esculentum var. cerasiforme) greenhouse crops (hybrids Shiren, Tomito, and Rubino top) in the areas of Drymos and Vonitsa, located at Aitoloakarnania Prefecture, in Greece. A total of 63 tomato samples (55 from symptomatic and 8 from asymptomatic plants) were collected from 11 greenhouses where disease incidence ranged from 10 to 20%. All samples were tested by double-antibody sandwich (DAS)-ELISA using polyclonal antibodies from BIOREBA, AG (Reinach, Switzerland) for the presence of PepMV, Cucumber mosaic virus (CMV), and Tomato mosaic virus (ToMV). Leaf tissue from PepMV-, CMV-, and ToMV-infected samples and virus-free tomato plants were included in all tests as positive and negative controls, respectively. Results showed that 53 symptomatic samples collected from all greenhouses were infected with PepMV and two were co-infected with PepMV and CMV. Total RNA was extracted from all infected plants with a commercially available kit (Qiagen, Hilden, Germany) and amplified by conventional and real-time reverse transcription (RT)-PCR, using previously reported protocols (2). Positive and negative controls were also included in each assay. The 200-bp amplified PCR fragments of Triple Gene Block 3 (TGB3) obtained from five infected samples were purified and both strands were sequenced. Sequencing data were analyzed, deposited in the GenBank, and compared with other reported sequences. In addition, leaf tissue from five samples infected with only PepMV was used for mechanical inoculation of four plants of Nicotiana glutinosa, N. benthamiana, and tomato (L. esculentum FA 179 hybrid) plants. As negative controls, two plants from each species were used. Sequencing analysis showed that all five PepMV sequences were identical (GenBank Accession Nos. FR686904 to FR686908) and possessed 100% identity PepMVstrain CH2 (DQ000985). Inoculation results showed that the virus was successfully transmitted to N. benthamiana and tomato plants which developed mosaic symptoms, and tested positive by DAS-ELISA and RT-PCR. N. glutinosa plants did not develop any symptoms and were found to be free of PepMV when tested by DAS-ELISA and RT-PCR. To our knowledge, this is the first report of PepMV in Greece. Further studies on the disease prevalence and incidence and its economic impact on tomato production are required. PepMV is currently under quarantine status in the EU and therefore new protective measures should be recommended to prevent the spread of PepMV to other regions of Greece. References: (1) I. M. Hanssen and B. P. H. J. Thomma. Mol. Plant Pathol. 11:179, 2010. (2) K. S. Ling et al. J. Virol. Methods 144:65, 2007.
Steroid-Induced Inflammatory Neuropathy in a Patient on Tumor Necrosis Factor-α Antagonist Therapy
Authors: Wong Siew L , Rajabally Yusuf A 
Published: November 2010

Abstract

We describe a patient on the tumor necrosis factor-α antagonist, adalimumab, for 2 years for rheumatoid arthitis, who developed a rapidly progressive inflammatory neuropathy shortly after starting oral steroids. Adalimumab was stopped at onset of neurologic symptoms. Electrophysiology showed demyelination, which persisted at 6 month follow-up, cerebrospinal fluid analysis showed persistent albuminocytologic dissociation, and magnetic resonance studies revealed enlarged and enhancing nerve roots. Treatment with intravenous immunoglobulins resulted in slow, progressive improvement. Tumor necrosis factor-α antagonists have previously been implicated in acquired demyelinating neuropathies. In this patient, we hypothesize that adalimumab may have caused an initially asymptomatic chronic inflammatory demyelinating polyneuropathy, which became symptomatic shortly after initiation of steroid therapy. This case may raise the issue of the safety of steroids in conjunction with antitumor necrosis factor-α therapy in susceptible patients.
Shards of the old looking glass: restoring the significance of identity in promoting positive outcomes for looked‐after children
Authors: McMurray Isabella , Connolly Helen , Preston‐Shoot Michael , Wigley Veronica 
Published: November 2010

ABSTRACTThere is a driving force within current UK child welfare policies which promotes a strengths led approach to both assessment and care planning for children in need. Social policy emphasizes the importance of tangible outcomes such as education achievement, which have been enshrined in statute. However, other developmental outcomes, such as identity, are subordinated. Notwithstanding the relevance of these more concrete indicators, this paper suggests that this focus has left a gap at theoretical, strategic and operational levels relating to more psychosocial aspects of human growth and development, such as identity. This paper seeks to redress that gap by considering identity as both a process and an outcome. Following a review of the literature on identity development, it presents an analysis of semi‐structured interviews with 13 young people and their social workers. The findings show how young people's identity is shaped by their relationships, can be a protective mechanism, and deferred or put on standby. In the concluding discussion the implications for social work practice are highlighted.
Talking out the rage: an ego‐supportive intervention for work with potentially abusive parents
Authors: Holman Warren D. 
Published: November 2010

ABSTRACTChild abuse experts agree that the single factor ultimately responsible for child maltreatment is the inability of parents to control their aggressive impulses. This paper describes a verbalizing, ego‐supportive intervention for work with potentially abusive parents. It is designed to give parents increased understanding of the experience of parenting and greater control over their angry impulses. The intervention is based on two well‐supported premises: anger towards one's children is natural and inevitable; and putting angry feelings and thoughts into words significantly diminishes the tendency to put them into action. The engagement, ego‐building and prevention benefits of the intervention are discussed.
Trade‐off between growth and immune function: a meta‐analysis of selection experiments
Authors: van der Most Peter J. , de Jong Berber , Parmentier Henk K. , Verhulst Simon 
Published: November 2010

Summary 1. Evidence suggests that developing and maintaining an effective immune system may be costly and that an organism has to make a trade‐off between immune function and other fitness‐enhancing traits. To test for a trade‐off between growth and immune function we carried out a meta‐analysis of data from lines of poultry that had been divergently selected for either growth (body mass) or an aspect of immune function. This is relevant to our understanding of the evolution of immune function, but also because the increased prevalence of antibiotic‐resistant bacteria and calls to restrict the use of antibiotics in the agricultural industry has made immune function of livestock an important theme. Has the selection of animals for rapid growth unintentionally resulted in reduced immune function? 2. The lines selected for increased growth all showed a strong and significant decrease in immune function (standard difference in means = 0·8; P < 0·001). No difference was found between the effects on cellular or humoral immunity, although there were few data on cellular immunity, and hence this deserves more study. However, in the lines selected for immune function the effect on growth was heterogeneous and overall it was close to zero. 3. Testing for publication bias revealed that the effect of selection for body mass on immune function was robust. However, there was considerable heterogeneity in both body mass and immune function data. The heterogeneity in the growth‐selected lines cannot be accounted for by gender or species: the only turkey line had an effect size between that of the two chicken lines. 4. In conclusion, we found that selection for growth does indeed compromise immune function, but selection for immune function did not consistently affect growth. This is in agreement with the supposition that the costs of growth are large relative to the costs of immune function, and on a practical level this suggests that it may be possible to breed animals for increased growth without loss of immune function.
The frequency and magnitude of non‐additive responses to multiple nutrient enrichment
Authors: Allgeier Jacob E. , Rosemond Amy D. , Layman Craig A. 
Published: November 2010

Summary1 Anthropogenic eutrophication is among the greatest threats to ecosystem functioning globally, often occurring via enrichment of both nitrogen (N) and phosphorus (P). As such, recent attention has focused on the implications of non‐additive responses to dual nutrient enrichment and the inherent difficulty associated with predicting their combined effects.2 We used a simple metric to quantify the frequency and magnitude of non‐additive responses to enrichment by N, P and N + P in 653 experiments conducted across multiple ecosystem types and locations.3 Non‐additive responses were found to be common in all systems. Freshwater ecosystems and temperate latitudes tended to have frequent synergistic responses to dual nutrient enrichment, i.e. the response was greater than predicted by an additive model. Terrestrial and arctic systems were dominated by antagonistic responses (responses to N + P that were less than additive).4 The mean of all experiments was synergistic because despite being less common, synergistic responses were generally of greater magnitude than antagonistic ones.5 Synthesis and applications. Our study highlights the ubiquity of non‐additive effects in response to dual nutrient enrichment and further elucidates the complex ways in which ecosystems respond to human impacts. Our results suggest how alternative nutrient limitation scenarios can be used to guide approaches to conservation and management of nutrient loading to ecosystems. This review provides the first published summary of non‐additive responses by primary producers.