bluuebunny/crossref_metadata_2025_split huggingface dataset:
Olfactory receptor signaling is regulated by the post‐synaptic density 95, Drosophila discs large, zona‐occludens 1 (PDZ) scaffold multi‐PDZ domain protein 1
Authors: Dooley Ruth , Baumgart Sabrina , Rasche Sebastian , Hatt Hanns , Neuhaus Eva M. 
Published: November 2009

The unique ability of mammals to detect and discriminate between thousands of different odorant molecules is governed by the diverse array of olfactory receptors expressed by olfactory sensory neurons in the nasal epithelium. Olfactory receptors consist of seven transmembrane domain G protein‐coupled receptors and comprise the largest gene superfamily in the mammalian genome. We found that approximately 30% of olfactory receptors possess a classical post‐synaptic density 95, Drosophila discs large, zona‐occludens 1 (PDZ) domain binding motif in their C‐termini. PDZ domains have been established as sites for protein–protein interaction and play a central role in organizing diverse cell signaling assemblies. In the present study, we show that multi‐PDZ domain protein 1 (MUPP1) is expressed in the apical compartment of olfactory sensory neurons. Furthermore, on heterologous co‐expression with olfactory sensory neurons, MUPP1 was shown to translocate to the plasma membrane. We found direct interaction of PDZ domains 1 + 2 of MUPP1 with the C‐terminus of olfactory receptors in vitro. Moreover, the odorant‐elicited calcium response of OR2AG1 showed a prolonged decay in MUPP1 small interfering RNA‐treated cells. We have therefore elucidated the first building blocks of the putative ‘olfactosome’, brought together by the scaffolding protein MUPP1, a possible central nucleator of the olfactory response.Structured digital abstract
MINT‐7290305: OR2AG1 (uniprotkb:Q9H205) physically interacts (MI:0915) with MUPP1 (uniprotkb:O75970) by anti tag coimmunoprecipitation (MI:0007)
MINT‐7289999, MINT‐7290250, MINT‐7290063, MINT‐7290110: OR2AG1 (uniprotkb:Q9H205) binds (MI:0407) to MUPP1 (uniprotkb:O75970) by peptide array (MI:0081)
MINT‐7290162: mOR283‐1 (uniprotkb:Q9D3U9) binds (MI:0407) to MUPP1 (uniprotkb:O75970) by peptide array (MI:0081)
MINT‐7290128: mOR‐EG (uniprotkb:Q920P2) binds (MI:0407) to MUPP1 (uniprotkb:O75970) by peptide array (MI:0081)
MINT‐7290219: hOR3A1 (uniprotkb:P47881) binds (MI:0407) to MUPP1 (uniprotkb:O75970) by peptide array (MI:0081)
MINT‐7290191: hOR1D2 (uniprotkb:P34982) binds (MI:0407) to MUPP1 (uniprotkb:O75970) by peptide array (MI:0081)
MINT‐7289922: AC3 (uniprotkb:Q8VHH7) and MUPP1 (uniprotkb:O75970) colocalize (MI:0403) by fluorescence microscopy (MI:0416)
MINT‐7289933, MINT‐7289954, MINT‐7289978: OR2AG1 (uniprotkb:Q9H205) binds (MI:0407) to MUPP1 (uniprotkb:O75970) by pull down (MI:0096)
Hurricane Disturbance Alters Secondary Forest Recovery in Puerto Rico
Authors: Flynn Dan F.B. , Uriarte María , Crk Tanja , Pascarella John B. , Zimmerman Jess K. , Aide T. Mitchell , Caraballo Ortiz Marcos A. 
Published: November 2009

ABSTRACTLand‐use history and large‐scale disturbances interact to shape secondary forest structure and composition. How introduced species respond to disturbances such as hurricanes in post‐agriculture forest recovery is of particular interest. To examine the effects of hurricane disturbance and previous land use on forest dynamics and composition, we revisited 37 secondary forest stands in former cattle pastures across Puerto Rico representing a range of exposure to the winds of Hurricane Georges in 1998. Stands ranged from 21 to>80 yr since agricultural abandonment and were measured 9 yr posthurricane. Stem density decreased as stands aged, while basal area and species richness tended to increase. Hurricane disturbance exerted contrasting effects on stand structure, contingent on stand age. In older stands, the basal area of large trees fell, shifting to a stand structure characteristic of younger stands, while the basal area of large trees tended to rise in younger stands with increasing hurricane disturbance. These results demonstrate that large‐scale natural disturbances can alter the successional trajectory of secondary forest stands recovering from human land use, but stand age, precipitation and soil series were better predictors of changes in stand structure across all study sites. Species composition changed substantially between census intervals, but neither age nor hurricane disturbance consistently predicted species composition change. However, exposure to hurricane winds tended to decrease the abundance of the introduced treeSpathodea campanulata, particularly in smaller size classes. In all sites the abundance of the introduced treeSyzygium jambosshowed a declining trend, again most strongly in smaller size classes, suggesting natural thinning through succession.
Beyond Tropical Forests Adoption: Contextualizing Conservation Strategies
Authors: Romero Claudia , Peña‐Claros Marielos 
Published: November 2009

ABSTRACTThe complexity of factors driving tropical deforestation demand integrated approaches from concerned researchers and policy makers. Strict protection is sometimes the most appropriate mode of conservation, but conservation through management is often the better option. In either case, this essay highlights the importance of considering the social/cultural, economic, and political contexts in which these forests are threatened. By attempting to understand the cultural settings, institutional architectures and dynamics, and local expectations, and then by combining the concepts and tools of a range of disciplines, researchers will be more likely to forge lasting partnerships and increase their potential for sustained improvement in resource management and overall forest conservation.
Passive Restoration in Biodiversity Hotspots: Consequences for an Atlantic Rainforest Lizard Taxocene
Authors: Guerrero Agustín Camacho , da Rocha Pedro Luís Bernardo 
Published: November 2009

ABSTRACTLong‐term conservation in biodiversity hotspots depends on the recovery of communities in secondary forest fragments. In most cases, however, recovery strategies for these areas are based only on passive restoration. It is therefore necessary to determine the efficiency of such strategies. In this study, we assess the efficiency of passive restoration on a 567‐ha 28‐yr‐old fragment of Atlantic Rainforest in Northeastern Brazil. We measured richness, composition, abundance and biomass of a lizard taxocene and also vegetation structure and availability of several microhabitat descriptors in 18 plots of this secondary forest. We then compared them with measures in 29 plots from two neighboring reference sites. Species richness, abundance, biomass and microhabitat descriptors availability inside the secondary fragment did not differ from reference sites. However, composition and vegetation structure showed small differences. Some forest specialist lizards, which should be a focus of conservation efforts in fragmented landscapes of the Atlantic Rainforest, were not found in the fragment and data indicate that this was not due to sampling or a lack of suitable habitat or microhabitat. In the presence of preserved source sites, passive restoration may be a cheap and effective way to recover lizard taxocenes of the Atlantic Rainforest. Some of the species may need to be re‐introduced to accelerate the full recovery of original composition of lizard taxocenes in secondary Atlantic Rainforests.
Annual Rainfall and Seasonality Predict Pan‐tropical Patterns of Liana Density and Basal Area
Authors: DeWalt Saara J. , Schnitzer Stefan A. , Chave Jérôme , Bongers Frans , Burnham Robyn J. , Cai Zhiquan , Chuyong Georges , Clark David B. , Ewango Corneille E. N. , Gerwing Jeffrey J. , Gortaire Esteban , Hart Terese , Ibarra‐Manríquez Guillermo , Ickes Kalan , Kenfack David , Macía Manuel J. , Makana Jean‐Remy , Martínez‐Ramos Miguel , Mascaro Joseph , Moses Sainge , Muller‐Landau Helene C. , Parren Marc P. E. , Parthasarathy Narayanaswamy , Pérez‐Salicrup Diego R. , Putz Francis E. , Romero‐Saltos Hugo , Thomas Duncan 
Published: November 2009

ABSTRACTWe test the hypotheses proposed by Gentry and Schnitzer that liana density and basal area in tropical forests vary negatively with mean annual precipitation (MAP) and positively with seasonality. Previous studies correlating liana abundance with these climatic variables have produced conflicting results, warranting a new analysis of drivers of liana abundance based on a different dataset. We compiled a pan‐tropical dataset containing 28,953 lianas (≥2.5 cm diam.) from studies conducted at 13 Neotropical and 11 Paleotropical dry to wet lowland tropical forests. The ranges in MAP and dry season length (DSL) (number of months with mean rainfall <100 mm) represented by these datasets were 860–7250 mm/yr and 0–7 mo, respectively. Pan‐tropically, liana density and basal area decreased significantly with increasing annual rainfall and increased with increasing DSL, supporting the hypotheses of Gentry and Schnitzer. Our results suggest that much of the variation in liana density and basal area in the tropics can be accounted for by the relatively simple metrics of MAP and DSL.
Le coup d'œil du spectateur: Spectatorial Function and Stage Space in French Theatre Design, 1760‐1784
Authors: CAMP PANNILL 
Published: November 2009

AbstractThe sightlines which architects such as Oppenord and Roubo the Younger drew on their theatre plans demonstrate an attempt to align stage spaces and optical fields. Indeed, the reform‐minded architects of the period 1748 to 1784 applied geometrical forms to their plans, and adopted a terminology borrowed from optics, the science of light and vision. In focusing specifically on the theoretical texts and architectural drawings published between 1765 and 1784, we argue that this use of optical space indicates a dislocation between, on the one hand, the spatial representation which the French reformers promoted in their drawings and, on the other, stage perspective, which had, since the previous century, been associated with the influence of the Italian Baroque. In the 1780s, architects gradually abandoned the use of stage perspective, preferring instead a theatrical space modelled after an ostensibly natural optical encounter.
De la police des spectacles à la civilisation des mœurs théâtrales: domestication du public et production des affects chez Louis‐Sébastien Mercier
Authors: POIRSON MARTIAL 
Published: November 2009

AbstractLouis‐Sébastien Mercier's conception of theatre revolves around the audience. He writes extensively about its social composition, its patterns of attendance and, in particular, the attitudes of parterre spectators. If Mercier goes furthest in acknowledging the audience, particularly the popular audience, as the ultimate judge of a performance, it is also he who betrays most explicitly the contradictions in his aesthetic and ideological stance. He places remarkable faith in the judgement of the people, arguing that the playwright should be subservient to a popular audience and that access to the theatre be made more democratic. He wants to see parterre audiences seated, for they alone are capable of judging the quality of the plays they see. He never misses a chance to taunt actors for their arrogance or authors for their pretensions. He believes in the educational value of a theatre that can inculcate civic and patriotic pride and can teach a popular audience its political responsibilities. And he pleads for the relaxation of police controls on playhouses as the only way to allow for the unhindered development of a more civil theatre culture. At the same time, however, he acknowledges that the creation of a ‘popular theatre’ is not easy, and is well aware of both the risks of manipulating or over‐controlling the audience and the dangerous potential inherent in exploiting theatricality to modify public opinion.
Copistes et secrétaires‐souffleurs à la Comédie‐Française au dix‐huitième siècle, de Saint‐Georges à Delaporte
Authors: RAZGONNIKOFF JACQUELINE 
Published: November 2009

AbstractThe numerous eighteenth‐century promptbooks conserved in the Bibliothèque‐Musée de la Comédie‐Française are for the most part in the hand of copyists attached to the company, people whose duties, often closely associated with those of prompters, gradually evolved over the course of the century. Traces of these people, who were as vital to the company as a whole as they were to individual actors who came to place great trust in them, may be discovered, scattered, not only throughout the minutes of company meetings and registers, but also in bills for copying work and other minor expenses incurred by the secretariat of the troupe. By collating these various sources it is possible to put a face to several of these figures, to identify the manuscripts and documents they copied out, the payment they received, and to understand exactly what their duties entailed over the course of the century. In addition, we shall consider the ‘outside’ copyists who were occasionally called upon, and those of the company's music copyists of whom we have evidence. After all, music in one form or another was an essential element of all eighteenth‐century performances.
Rousseau and Favart at Fontainebleau, Pergolesi at Versailles
Authors: CHARLTON DAVID 
Published: November 2009

AbstractThe French court, far from being caught off guard by the arrival of the Bouffons in 1752, had in fact been pursuing an independent policy which favoured the performance of operas since 1745. In 1749, it was its taste for spectacle that prompted the court to purchase buildings at Fontainebleau which would make it possible to house the staffs of the Royal Entertainments Department (les Menus‐Plaisirs), the workshops and stage properties departments, and to install a small rehearsal room. Three years later, Louis Bays de Cury, who was in overall charge of the Royal Entertainments Department, seized the opportunity of putting on Rousseau's Le devin du village. Two documents preserved in the French National Archives (O1 2991 and O1 2992) reveal the details not only of the extraordinary expenses incurred in the journeys to Fontainebleau, but also the particularly mixed styles of programming. They also give details of the costumes made for Le devin and other performances, which in some instances enhance our understanding of the stage practice of the Italian actors. In 1752, the passion for opera spread from Fontainebleau to Versailles, where the stage of the Salle de Comédie, located in the south wing of the palace, was subsequently enlarged in order to house not only the Bouffons, but also to permit the staging of Zélindor, roi des Silphes, a ballet by Moncrif, Rebel and Francœur. Zélindor was also staged at Versailles in 1745, but that was on a stage erected in the Grande Ecurie, which was destroyed by fire in 1751.
HYPERTENSION MANAGEMENT: LIFESTYLE INTERVENTIONS IN A TRANSCULTURAL CONTEXT
Authors: Ho Tai Mooi 
Published: November 2009

SUMMARYHypertension is a risk factor for cardiovascular and kidney diseases. According to estimation, the prevalence of hypertension will increase unless extensive and effective preventive measures are implemented. The diversity of languages and cultures of the hypertensive patients requiring adequate blood pressure control make communications difficult in many instances.Nursing intervention for patients to adopt a healthy lifestyle requires effective communication. But the communication problems encountered in a culturally diverse context can result in undesirable outcomes for the patients and the health‐care team. This paper describes the production of a document to assist staff address the difficulty in intercultural communication, which could be used anywhere in the world. This document can facilitate nursing intervention to achieve optimal hypertension management in a transcultural context, responding to the challenge regarding preventive measures to halt increase in hypertension prevalence.
THE AUSTRALIAN AND NEW ZEALAND DIALYSIS WORKFORCE STUDY IN THE INTERNATIONAL CONTEXT
Authors: Polaschek Nick , Bennett Paul N. , McNeill Liz 
Published: November 2009

SUMMARY Background: Given increasing demand for renal replacement therapy, this study sought to identify of key workforce issues facing dialysis units, based on a “snapshot” of the current workforce. Methods: A web‐based survey of all dialysis unit managers in Australia and New Zealand, in October 2008, about their workforce. Results: A significant minority of dialysis staff in most regions were not registered nurses. Many renal registered nurses worked part‐time. Staff/patient ratios in dialysis units varied significantly by region, reflecting the relative prevalence of home therapies. Most dialysis units were generally adequately staffed. The proportion of registered nurses with specific renal qualifications varied significantly by region. Conclusion: The changing character of the workforce in the dialysis unit in the future will require clarification of the relationships between different categories of dialysis staff. Specialty education for nurses needs to be oriented to equipping staff to be effective in their changing work environment.
USE OF GADOLINIUM FOR CAROTID ARTERY ANGIOGRAPHY AND STENTING IN PATIENTS WITH RENAL INSUFFICIENCY
Authors: Yaganti Vamsee , Alani Firas , Yaganti Sushmita , Goldberg Sheldon , McCormick Daniel 
Published: November 2009

SUMMARYUse of iodinated contrast media (ICM) for angiography can result in contrast‐induced nephropathy (CIN). Gadolinium‐based contrast media (GCM) have been used in angiography with a goal to reduce the incidence of CIN. We performed a retrospective analysis involving 85 patients with renal insufficiency who underwent 97 carotid artery angiography and stenting (CAAS) procedures with a combination of GCM and ICM. The incidence of peri‐procedural death, Q wave myocardial infarction (QWMI), stroke and CIN were recorded. Patients in GCM group had worse preprocedure renal function compared to ICM group. There were no peri‐procedural deaths or QWMI in both groups. The incidence of stroke was 2.2% in GCM group and 0% in ICM group. The incidence of CIN were similar in GCM and ICM groups (8.5% vs. 10%, respectively, p NS). However, the predicted risk of CIN was 18.5% for GCM group and 10.4% for ICM group. Use of GCM and ICM combination for CAAS resulted in a 50% reduction in the incidence of predicted CIN risk compared to ICM.
Catch Wind of This
Authors: Riendeau Diane 
Published: November 2009

This month, I couldn't resist putting together a suite of sweet videos that involve wind (or air). The videos show some of the ways we have learned to harness the wind (or air resistance) to perform feats. Some show how the wind affects us and our adaptations because of this!
Special thanks to Jim Hicks, Barrington High School (retired), Barrington, IL; Shannon Mandel, Barrington High School, Barrington, IL; Jennifer Groppe, Maret School, Washington, D.C.; and Mike Bush, Libertyville High School, Libertyville, IL.
Dispersal of Carbapenemase
bla
VIM-1
Gene Associated with Different Tn
402
Variants, Mercury Transposons, and Conjugative Plasmids in
Enterobacteriaceae
and
Pseudomonas aeruginosa
Authors: Tato Marta , Coque Teresa M. , Baquero Fernando , Cantón Rafael 
Published: November 2009

ABSTRACT

The emergence of
bla
VIM-1
within four different genetic platforms from distinct
Enterobacteriaceae
and
Pseudomonas aeruginosa
isolates in an area with a low prevalence of metallo-β-lactamase producers is reported. Forty-three VIM-1-producing isolates (including 19
Enterobacter cloacae
, 2
Escherichia coli
, and 2
P. aeruginosa
isolates, 18
Klebsiella pneumoniae
isolate, and 2
Klebsiella oxytoca
isolate) recovered from 2005 to 2007 and corresponding to 15 pulsed-field gel electrophoresis types were studied. The
Enterobacteriaceae
isolates corresponded to a hospital outbreak, and the
P. aeruginosa
isolates were sporadically recovered. The genetic context of the integrons carrying
bla
VIM-1
(arbitrarily designated types A, B, C, and D) was characterized by PCR mapping based on known Tn
402
and mercury transposons and further sequencing. Among
Enterobacteriaceae
isolates,
bla
VIM-1
was part of integrons located either in an In2-Tn
402
element linked to Tn
21
(type A; In110-
bla
VIM-1
-
aacA4-aadA1
) or in a Tn
402
transposon lacking the whole
tni
module [type B; In113-
bla
VIM-1
-
aacA4-dhfrII
(also called
dfrB1
)-
aadA1-catB2
] and the transposon was associated with an IncHI2 or IncI1 plasmid, respectively. Among
P. aeruginosa
isolates,
bla
VIM-1
was part of a new gene cassette array located in a defective Tn
402
transposon carrying either
tniB
Δ
3
and
tniA
(type C;
bla
VIM-1
-
aadA1
) or
tniC
and Δ
tniQ
(type D;
bla
VIM-1
-
aadB
), and both Tn
402
variants were associated with conjugative plasmids of 30 kb. The dissemination of
bla
VIM-1
was associated with different genetic structures and bacterial hosts, depicting a complex emergence and evolutionary network scenario in our facility, Ramón y Cajal University Hospital, Madrid, Spain. Knowledge of the complex epidemiology of
bla
VIM-1
is necessary to control this emerging threat.
Effects of the Combination of Favipiravir (T-705) and Oseltamivir on Influenza A Virus Infections in Mice
Authors: Smee Donald F. , Hurst Brett L. , Wong Min-Hui , Bailey Kevin W. , Tarbet E. Bart , Morrey John D. , Furuta Yousuke 
Published: November 2009

ABSTRACTFavipiravir (T-705 [6-fluoro-3-hydroxy-2-pyrazinecarboxamide]) and oseltamivir were combined to treat influenza virus A/NWS/33 (H1N1), A/Victoria/3/75 (H3N2), and A/Duck/MN/1525/81 (H5N1) infections. T-705 alone inhibited viruses in cell culture at 1.4 to 4.3 μM. Oseltamivir inhibited these three viruses in cells at 3.7, 0.02, and 0.16 μM and in neuraminidase assays at 0.94, 0.46, and 2.31 nM, respectively. Oral treatments were given twice daily to mice for 5 to 7 days starting, generally, 24 h after infection. Survival resulting from 5 days of oseltamivir treatment (0.1 and 0.3 mg/kg/day) was significantly better in combination with 20 mg/kg of body weight/day of T-705 against the H1N1 infection. Treatment of the H3N2 infection required 50 mg/kg/day of oseltamivir for 7 days to achieve 60% protection; 25 mg/kg/day was ineffective. T-705 was ≥70% protective at 50 to 100 mg/kg/day but inactive at 25 mg/kg/day. The combination of inhibitors (25 mg/kg/day each) increased survival to 90%. The H5N1 infection was not benefited by treatment with oseltamivir (≤100 mg/kg/day for 7 days). T-705 was 30 to 70% protective at 25 to 100 mg/kg/day. Survival improved slightly with combination treatments. Increased activity was seen against H5N1 infection by starting treatments 2 h before infection. Oseltamivir was ineffective at ≤40 mg/kg/day. T-705 was 100% protective at 40 and 80 mg/kg/day and inactive at 20 mg/kg/day. Combining ineffective doses (20 mg/kg/day of T-705 and 10 to 40 mg/kg/day of oseltamivir) afforded 60 to 80% protection and improved body weights during infection. Thus, synergistic responses were achieved with low doses of T-705 combined with oseltamivir. These compounds may be viable candidates for combination treatment of human influenza infections.