bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of culture time on maintenance of limbal epithelial progenitors in the explant culture system
Authors: GHOUBAY D , SANDALI O , GOLDSCHMIDT P , BULLET J , LAROCHE L , BORDERIE V 
Published: August 2012

AbstractPurpose To assess the influence of culture time on maintenance of limbal epithelial progenitors in the explant culture system.Methods Limbal epithelial cells were cultured from 2‐mm2 human limbal explants in cholera toxin‐free medium with no feeders for 9‐21 days. Expression of differentiation and progenitor markers was assessed by immunochemestry and RT‐PCR. The clonal growth ability of cultured epithelial cells at each time of culture and with murine 3T3 feeders or human corneal stromal feeders was evaluated by determining colony forming efficiency (CFE).Results The average number of cultured cells obtained after 7, 14, and 21 days was, respectively, 500, 60489, and 195949. Expression of broad spectrum cytokeratins, CK3, CK19, vimentin, p63, and ABCG‐2 was observed at all culture time points by immunocytochemistry and RT‐PCR. Epithelial colonies were observed at 9, 11, 14 and 18 of culture but not at 21 days, both with murine 3T3 feeders and human corneal stromal feeders. Higher number of colonies was obtained when limbal epithelial cells were cultured with murine 3T3 feeders than with human corneal stromal feeders. The highest number of colonies was obtained at 14 days of culture with murine 3T3 feeders. The average cell Feret diameter (µm)/circularity for clones obtained from 11‐, 14‐, and 18‐day primary cultures were, respectively, 38.4/0.76, 34.2/0.80, and 45.7/0.72 (p<0.0001). The average percentage of p63+ small cells was, respectively 2.6%, 4.7%, and 1.3% in clones obtained from 11‐, 14‐, and 18‐day primary cultures (p=0.002).Conclusion In the explant culture system, limbal epithelial progenitors are better preserved after 2 versus 3 weeks of culture.
Targeting host kinases for the treatment of herpes keratitis
Authors: ALEKSEEV O , JENNINGS S , AZIZKHAN‐CLIFFORD J 
Published: August 2012

AbstractPurpose Herpes keratitis (HK) is a common cause of blindness in the developed world. A large number of HK cases are refractory to antiviral agents and ultimately result in permanent corneal damage. The purpose of this study is to identify new therapeutic targets against HSV and to assess their antiviral potential in tissue culture experiments, as well as in more sophisticated models of HK. Specifically, we focused on the involvement of ataxia telangiectasia mutated (ATM) and its downstream target Chk2 in facilitating productive HSV infection in corneal epithelium.Methods We used human corneal epithelial cell lines – hTCEpi and HCE – as in vitro models of corneal HSV infection, and also developed an ex vivo model of acute corneal epithelial infection, where explanted human and rabbit corneoscleral buttons were infected and maintained in organ culture. We are currently utilizing an in vivo mouse model of ocular herpes to further validate our findings. Small molecule inhibitors of ATM (KU‐55933, wortmannin, caffeine) and Chk2 (Chk2 inhibitor II), as well as RNAi against ATM and Chk2, were used to inhibit these two kinases. We used plaque assays and qPCR to assess the infectious particle production, genome replication, and transcriptional activity of HSV in corneal epithelium.Results Small molecule or RNAi‐mediated inhibition of ATM or Chk2 greatly suppressed the replication and transcription of the viral genome, as well as the infectious particle production. This was observed in the tissue culture models and, importantly, in organotypically explanted human and rabbit corneas. Results of the animal studies will also be presented.Conclusion This study identifies two host kinases – ATM and Chk2 – as potential novel therapeutic targets against herpes keratitis.
Biocompatible of human acellular cornea matrix
Authors: NASINNYK I , PASYECHNIKOVA N , LEUS M 
Published: August 2012

AbstractPurpose The aim of this study was to construct a rabbit anterior cornea replacement with an acellular human cornea matrix (AHCM) as a scaffoldMethods Cornea was taken from the enucleated eye from patient with melanoma of choroidea. Before the decellularization process, human corneas were washed 3 times in sterile phosphate buffered saline. To remove the hereditary materials, the cornea discs were immersed in a Triton X 100 solution and Ammonium Hydroxide with a solvent/tissue mass ratio of 20:1 (vol./wt.). AHCMs were transplanted into the right corneas of 9 Chinchilla rabbits. The superficial corneas of the right eye of the rabbits were excised with a 6‐mm punch. AHCM lamellas (150 mm, 6 mm diameter) were made using a microkeratome. AHCM were transplanted and fixed with 10‐0 nylon into rabbit corneas. All rabbits received topical levofloxacin eye. The non‐operated, contralateral eye was used as a positive control. Follow‐up clinical examinations included slit‐lamp examination to assess corneal optical clarity, neo‐vascularization and degradation of grafts. After post‐operative 8 weeks, rabbits were euthanized respectively and the corneal specimens were examined by H&E stainingResults After lamellar implantation of the AHCM, all animals survived without infections or other complications during the follow‐up period. The transplanted AHCM could be recognized in the rabbit corneas at 8 weeks after operation, with no neo‐vascularization and inﬂammation or any other rejection signs in or around the transplanted disks, as demonstrated by H&E staining. H&E staining showed that the implants were well integrated within the host corneas, with cells inﬁltrating the transplanted disks.Conclusion Acellular human cornea matrix has a good biocompatible properties after lamellar keratoplasty
Treatment of ligneous conjunctivits with subconjunctival fresh frozen plasma (ffp): about three severe cases
Authors: GABISON E , DOAN S , AJZENBERG N , COCHEREAU I 
Published: August 2012

AbstractPurpose Ligneous conjunctivitis is a rare type of chronic membranous conjunctivitis. It is inherited as an autosomal recessive disorder, with low plasminogen levels reported in both homozygous and heterozygous individualsMethods we describe the clinical features and response to subconjunctival FRESH FROZEN PLASMA (FFP) in three patients with severe ligneous conjunctivitis with a mean follow‐up of 12 months, among 15 patients followed in our department for the past 10 years.Results All our cases had bilateral ocular involvement except. They were all treated with subconjunctival injection of FFP after resection of the membranes. Topical heparin and rimexolone application was continued 1 month after surgery. As the case 2 presented a corneal perforation during the course of the disease, he was also treated with multiple inlay amniotic membrane graft.Conclusion FFP shortens the treatment period, reduces the probability of mid‐term recurrences and is a good alternative to a long‐term topical treatment with Heparin and steroid.
Cultivating oral mucosa epithelial transplantation (COMET) followed by penetrating keratoplasty (PK) in ocular surface reconstruction
Authors: DOBROWOLSKI D , WOWRA B , ORZECHOWSKA‐WYLEGALA B , WYLEGALA E 
Published: August 2012

AbstractPurpose Efficacy of COMET and subsequent PK in patients with limbal insufficiency combined with stromal corneal haze after corneal burnsMethods 15 patients (16 eyes)who underwent corneal burns with vascular conjuctival pannus and stromal involvement underwent autologous cultivated oral mucosa epithelial transplantation. Cellular material was obtained from buccal mucosa, tripsinized, and settled on the previously prepared culture dishes. Cultures was carried on denuded amniotic membrane in presence of inactivated with mitomycin C 3T3 fibroblasts. At least 12 month after epithelium restoration patients underwent PK. Corneal surface stability and visual acuity were evaluated.Results 6 month after last step of the surgery 68,7% of eyes had transparent cornea without reccurent revascularization, 31,2 % of eyes developed vascular pannus again with vascular ingrowth on the transplanted cornea ( in 2 cases visual axis was involved), in 62,5 % of eyes visual acuity increased from mean 0,01 to 0,3.Conclusion COMET combined with PK effectively improves ocular surface stability and quality of vision.
Comparative study of three exophthalmometers and computed tomographic biometry
Authors: DELMAS J , MARTIN S , LOUSTAU JM , BOURMAULT L , ADENIS JP , ROBERT PY 
Published: August 2012

AbstractPurpose Accurate and reproducible exophthalmometry is recognized as having a great importance in the clinical setting. However many variations are described among the different commercially available exophthalmometers.Methods In this prospective study, 3 exophthalmometers (Luedde’s, 2 mirrors Hertel’s, and 1 prism Mourits’ exophthalmometers) were compared to tomodensitometric biometry by 2 observers, for 60 patients. Data recorded were the external prebicanthal segment (EPBCS), the inter‐orbital distance (IOD) and the axial length of the globe (AL). Intraobservers and interobservers reproducibility were evaluated, using intraclass correlation coefficient (ICC) and Bland and Altman method.Results Concerning agreement between EPBCS measurements and CT‐scan biometry, ICC for Luedde’s instrument was 0.59 and 0.64 respectively for the 2 observers, for Hertel’s exophthalmometer: 0.63 and 0.72 and for Mourits’exophthalmometer: 0.94 and 0.87. Intraobserver and interobservers reproduciblities were high, particularly for Mourits’ exophthalmometer, concerning EPBCS and IOD measurements. Moreover, Bland and Altman charts showed that Luedde’s instrument induced underestimation of the readings, Hertel’s exophthalmometer overestimated small values and underestimated high values, and Mourits’ exophthalmometer slightly underestimated the readings compared to CT‐scan biometry.Conclusion We showed a greater accuracy with 1 prism Mourits’ exophthalmometer, whereas Luedde’s instrument has a moderate agreement with CT‐scan biometry and Hertel’s exophthalmometer intermediate results. Intra‐ and interobservers reliability were good. International selection of reliable exophthalmometers should be defined for clinical practice and studies.
Vincristine‐induced unilateral ptosis
Authors: BATTA B , TRECHOT F , GEORGE JL , ANGIOI K 
Published: August 2012

AbstractPurpose To report the ocurrence of an unilateral ptosis in a 2‐year‐old girl treated by vincristine for a vaginal rhabdomyosarcoma.Methods Our young patient developed a vaginal rhabdomyosarcoma and had been treated by chemotherapy (Ifosfamide‐Vincristine‐Actinomycine) when an unilateral ptosis occurred 7 days after the fifth vincristine dose (1.5 mg/m2). At that time, cumulative vincristine dose was 4.50 mg. Neither oculomotor dysfunction nor anisocoria were present. Neurological and systemic examinations were otherwise unremarkable. Laboratory testing and thoracic‐cervical‐cranial scan were normal. Other causes of ptosis were excluded. Despite vincristine dose was reduced by a third, ptosis slightly increased after the 2 subsequent injections but decreased between cures. Ptosis disappeared at day 78 and no recurrence was noticed after the treatment continuation at the same dose.Results Neurotoxicity is a well‐known complication of vincaalkaloids. Bilateral ptosis is relatively common but reported cases of vincristine‐induced unilateral ptosis are infrequent. In our young patient, occurrence of the ptosis during treatment, increase during new infusions of vincristine and exclusion of other aetiologies are forceful arguments to consider the role of vincristine in this side effect. The use of pyridoxine or pyridostigmine were suggested to treat vincristine‐induced cranial neuropathies but to date, the benefit remains unproved. Adaptation of treatment regimen must be done. In our case dose reduction allowed disappearance of the ptosis.Conclusion Even if unilateral ptosis is rare among vincristine‐induced neurological complications, ophthalmologists must be aware of this diagnosis.
Jugular venous thrombosis secondary to idiopathic myelofibrosis: a rare cause of bilateral papilledema
Authors: MATEO J , JIMENEZ B , ASCASO F , LAVILLA L , PEIRO C , IBANEZ J 
Published: August 2012

AbstractPurpose Idiopathic myelofibrosis is a chronic myeloproliferative disorder in which the bone marrow is progressively substituted by connective fibrous tissue.This is due to an increase of the fibroblast growth factor, produced by the megakaryocytes.Among its signs and symptoms, we can find extramedullary hematopoiesis with hepatosplenomegally, anemia, weight loss, bone pain, infections or coagulopathy.Bone marrow biopsy shows different degrees of fibrosis and nests of megakaryocytes.Patients receive mainly symptomatic treatment and,sometimes,chemotherapy.Methods We present the case of a 65‐year‐old woman diagnosed with idiopathic myelofibrosis.She complained of bilateral loss of vision after suffering a right internal jugular vein thrombosis.Her best corrected visual acuity was 20/40 in the right eye and 20/200 in the left eye.She developed a severe bilateral papilledema and macular edema, as well as retinal hemorrhages and peripapillary cotton wool spots.Results The patient was treated with systemic steroids and anti‐platelet therapy, showing great improvement, with a final visual acuity of 20/25 in the right eye and 20/30 in the left eye.The macular edema was recovered, along with the visual fields.The papilledema was significantly reduced after the treatment.She is now being treated with heparin and her visual acuity remains stable.Conclusion Jugular thrombosis is an uncommon cause of papilledema. Idiopathic myelofibrosis often causes platelet disorders which may lead either to thrombosis or easy bleeding.Ophthalmologists should be aware of internal jugular vein thrombosis as a possible cause of bilateral papilledema in patients suffering from diseases that can cause blood coagulation abnormalities.
Contribution of MRI in cases of isolated mydriasis: description of neurovascular conflicts.
Authors: TRECHOT F , BRAUN M , CLOCHE V , ANGIOI K 
Published: August 2012

AbstractPurpose To study the contribution of MRI (sequence FIESTA) in the analysis of isolated and persistant mydriasis.Methods Prospective single center study (November 2010 to March 2012) including patients with unilateral mydriasis (pure intrinsic Cranial Nerve III palsy) without life‐threatening pathology. Complete ophthalmological, systemic and neurological examinations were performed. All patients with any symptoms of extrinsic CN III palsy (diplopia, ptosis or ophthalmoplegia), with tonic pupil (pilocarpine diluted test positive), use of toxic or drug taking, sign of focus at neurological examination have been excluded. Our patients underwent cerebral MRI: images acquisition were performed on a GE 3T MRI, protocol included FIESTA and 3D TOF sequences.Results Five patients were included (20 to 41 years), 2 women. They presented with a single non reactive, isolated and persistent mydriasis with a pilocarpine diluted‐test negative. For all, an obvious relationship between CN III and its adjacent Posterior Comunicating Artery (PCA) was demonstrated through FIESTA sequences with MPR images (3 real neurovascular conflicts in Root Entry Zone, 1 before cavernous sinus, 1 compression between PCA and dorsum selae). No saccular aneuvrysm or tumors were found.Conclusion Thanks to MRI, we explained the symptoms for all the five patients, because of anatomical features, including neurovascular conflicts. If it has been described previously for facial and trigeminal nerves, no neurovascular conflict between CN III and PCA has been demonstrated before. The contribution of MRI in the study of anisocoria seems to be considered, and could help for diagnostic of a pathology tagged idiopathic.
Retina thickness measurements provide a sensitive and specific diagnostic tool in Parkinson’s disease
Authors: GARCIA MARTIN E , BAMBO M , PRIETO E , DE LA MATA G , FERNANDEZ‐PEREZ S , SATUE M , IDOIPE M , FUERTES I , OTIN S , ALARCIA R , ROCHE JC , SERAL M , POLO V , LARROSA JM , PABLO L 
Published: August 2012

AbstractPurpose To test the diagnostic ability of optical coherence tomography (OCT) for the detection of Parkinson’s disease (PD). Retinal thickness depends in part on the retinal pigment epithelium, one of the principal tissues producing levodopa.Methods PD patients (n=111) and healthy subjects (n=200) were enrolled. The Axonal application of the Spectralis OCT was used to obtain the circumpapillary RNFL thickness and retinal measurements in each participant. Two linear discriminant functions (LDF) were developed, one using RNFL parameters and another LDF using retinal thickness. A validating set (120 eyes from healthy individuals and 66 eyes from PD patients) was used to test the performance of both LDFs in an independent population. Receiver operating characteristic (ROC) curves were plotted and compared between both LDFs and with the standard parameters provided by OCT. Sensitivity and specificity were used to evaluate diagnostic performance.Results The Retinal LDF combines only retinal parameters and provided the best performance: 31.1 + 0.02 x Temporal Outer ‐ 0.2 x Superior Outer + 0.16 x Nasal Outer ‐ 0.2 x Inferior Outer ‐ 0.06 x Superior Inner + 0.05 x Foveal thickness. The largest areas under ROC curve were 0.902 for Retinal LDF and 0.845 for RNFL LDF. At 95% fixed specificity, the Retinal LDF yielded the highest sensitivity values.Conclusion Measurements of retinal thickness obtained with Spectralis OCT had a very good ability to differentiate between healthy and PD individuals. Based on the area under the ROC curve, the Retinal LDF performed better than any single parameter or diagnostic test used for PD.
Retinal circulation and intracranial pressure in idiopathic intracranial hypertension
Authors: PIERRU A , VIGNAL C , GENEVOIS O , PAQUES M 
Published: August 2012

AbstractPurpose Idiopathic Intracranial Hypertension (IIH) is associated with moderate to severe papilledema (PE). Cases of central retinal vein oclusion identified in IIH suggest that PE can obstruct venous flow. The aim of this study was to evaluate the effect of intracranial hypertension on venous circulation and to determine the correlation between vascular caliber and intracranial pressure level.Methods In this retrospective multicenter study, we included 8 patients with newly diagnosed IIH. Fundus photography were performed before and after lumbar puncture at various time points. The images were aligned with i2k pro© software. Arterio‐veinous ratio (AVR) was measured by a dedicated software.Results Immediately following lumbar puncture these was decrease of the diameter of the central retinal vein. In 4 patients, an associated decrease in venous tortuosity was observed. For all patients, AVR increased immediately after lumbar puncture. This was due to the combined effect of a decreased venous diameters and an increase of the arterial caliber.Conclusion Venous diameters may be an interesting indicator of PE evolution. Fundus photography are a non‐invasive, convenient method to investigate and monitor IIH which may be useful in a variety of setting, including neurosurgery units. A prospective study is necessary to confirm these results.
Visual function improvement after idebenone therapy in Leber hereditary optic neuropathy
Authors: ORSSAUD C , ROBERT M , ROCHE O , DUFIER JL 
Published: August 2012

AbstractPurpose There is still no therapy for patients with Leber hereditary optic neuropathy (LHON). Recent studies have confirmed the effectiveness of Idebenone for the treatment of specific groups of LHON patients. However, it is still unclear if this treatment can be interesting in all cases of LHON. We wanted to evaluate whether Idebenone therapy in association with vitamin B2 and vitamin C can modify the visual function in non selected LHON patients.Methods A retrospective review of visual acuity and Goldmann visual field performed in patients referred to the ophthalmology clinic for LHON before and after a therapy with Idebenone (up to 270 mg/day), vitamin B2 and vitamin C.Results Seventy five patients were included. Mean duration between LHON onset and inclusion was 36 months. Mean treatment duration was 25 months. Twenty seven per cent of patients presented visual improvement. A surface reduction of the central scotoma greater than 30 % was observed in 36.2 % of these patients.Conclusion There is an effect of Idebenone therapy on visual function of LHON patients. Due to the delay between symptoms onset and beginning of the treatment, improvement does not seem to be due to habituation to low vision. However, Idebenone therapy is unable to correct visual disability in LHON population.
Analysis of changes in the macula and retinal nerve fiber layer by optical coherence tomography in patients with Alzheimer’s disease (AD)
Authors: CRUZ N , CASAS P , ASCASO F , CRISTOBAL JA , DEL BUEY MA , MINGUEZ E 
Published: August 2012

AbstractPurpose To evaluate the usefulness of optical coherence tomography (OCT) in the diagnosis of Alzheimer’s disease(AD) Thickness is determined at the peripapillary RNFL and macular volume with OCT, in individuals suffering from AD and compared with a control group.Methods Prospective study in 40 eyes of 20 patients with Alzheimer’s disease and 40 eyes of 20 controls, measuring the thickness of the peripapillary RNFL and macular volume with OCT.Results The average thickness of RNFL in patients with Alzheimer’s disease was 65,28±11,55 microns (53,50 to 87,29), significantly decreased compared to control subjects, which was 107.1 ± 7, 2 microns (94.9 ‐128.8) (p = 0.0001. Mann‐Whitney U test.). Macular volume in patients with AD was 5.6 ± 0.3 mm 3 compared with control subjects: 7.0 ± 0.5 mm3. Peripapillary RNFL thickness evaluated in each of the separate quadrants (superior, inferior, nasal and temporal) is also statistically significantly decreased in patients with AD in connection with control subjects. However, the central foveal thickness (1 mm), thickness macular inner ring (3 mm) and macular volume were significantly higher in AD patients.Conclusion The AD is the major cause of dementia in the world. Although the entorhinal cortex and hippocampal complex are best known as the sites of early pathology in AD, increasing evidence shows that the eye, particularly the retina, is also affected. The AD‐related changes in the retina are associated with degeneration and loss of neurons, reduction of the retinal nerve fibres, increase in optic disc cupping, retinal vascular tortusity and thinning, and visual functional impairment.