bluuebunny/crossref_metadata_2025_split huggingface dataset:
Platelet-rich Plasma Reverses the Inhibition of Tenocytes and Osteoblasts in Tendon–Bone Healing
Authors: Zhai Wenliang , Wang Nan , Qi Zhi , Gao Quan , Yi Liren 
Published: April 2012

The purpose of this study was to investigate the effect of platelet-rich plasma on the proliferation of osteoblasts and tenocytes in tendon–bone healing. We cultured osteoblasts and tenocytes in an indirect coculture system with Transwell filters (Merck Millipore, Billerica, Massachusetts). The proliferation was examined using Cell Counting Kit-8 (Dojindo Chemistry Research Institute, Kumamoto, Japan).Four groups were studied: group 1, one cell type cultured without platelet-rich plasma; group 2, two cell types cultured together in an indirect coculture system without platelet-rich plasma; group 3, cells in the outer chamber and platelet-rich plasma in the inner chamber; and group 4, two different cell types in each of the 2 chambers with platelet-rich plasma in the inner chamber. The proliferation rates of groups 3 and 4 were the highest, followed by group 1 and then group 2, which was the lowest.Platelet-rich plasma abolishes the inhibition of osteoblasts or tenocytes in an indirect coculture system and improves the cell proliferation rate.
Expansive Open-door Laminoplasty With Titanium Miniplate Versus Sutures
Authors: Chen Guangdong , Luo Zongping , Nalajala Badri , Liu Tao , Yang Huilin 
Published: April 2012

Expansive laminoplasty is an effective treatment for cervical myelopathy. Since the design of classic open-door laminoplasty with the use of suture, the procedure has been modified to reduce complications such as restenosis, axial symptoms, and segmental motor paralysis. Expansive open-door laminoplasty with the use of titanium miniplate is becoming popular. It is effective in expanding spinal canal dimensions with good clinical efficacy. However, a lack of studies exist comparing titanium miniplate fixation with classical suture fixation.
We performed a retrospective study of 54 patients with cervical myelopathy. Twenty-nine patients (4 women and 25 men) receiving expansive open-door laminoplasty by titanium miniplate fixation were classified as the modified group, and 25 patients (5 women and 20 men) fixed with suture served as the control group. Clinical and radiologic outcomes were assessed. No significant differences were observed in Japanese Orthopaedic Association scores and the recovery rate of C5 palsy. The incidence of axial symptoms in the modified group was significantly lower than that in control group. Radiologic examination showed that postoperative C2–C7 lordosis and range of motion of the cervical spine in the modified group were preserved. No significant differences were observed in mean anteroposterior diameter and open angle in the 2 groups. Both surgical protocols were effective in preventing reclosure of open laminae. Furthermore, the modified laminoplasty was superior in reducing the incidence of axial symptoms and loss of cervical lordosis and range of motion.
Familial Association of Femoral Trochlear Dysplasia With Recurrent Bilateral Patellar Dislocation
Authors: Rebolledo Brian J. , Nam Denis , Cross Michael B. , Green Daniel W. , Sculco Thomas P. 
Published: April 2012

Femoral trochlear dysplasia is an anatomic deformity that predisposes patients to patellar instability, including patellar subluxation and dislocation, and can lead to severe patellofemoral joint degeneration if left untreated. Femoral trochlear dysplasia leading to recurrent bilateral patellar dislocation has rarely been reported as having a familial association. Orthopedic surgeons who encounter patients presenting with chronic patellar instability with no underlying disease or syndrome should be aware of the presence of femoral trochlear dysplasia leading to recurrent bilateral patellar dislocation. Although femoral trochlear dysplasia remains uncommon, the presence of bilateral recurrent patellar dislocation in multiple members of the same family is highly suggestive of genetic inheritance.
This article describes 3 patients from 1 family who presented with femoral trochlear dysplasia leading to recurrent bilateral patellar dislocation. To our knowledge, this is the second article to describe a familial form of femoral trochlear dysplasia associated with recurrent bilateral patellar dislocation and is the first article in English. A lower threshold for screening and early intervention for symptomatic family members may be indicated to prevent the long-term effects of chronic patellar subluxation, dislocation, and patellofemoral arthritis.
Collagen Type V Polymorphism in Spontaneous Quadriceps Tendon Ruptures
Authors: Galasso Olimpio , Iaccino Enrico , Gallelli Luca , Perrotta Ida , Conforti Francesco , Donato Giuseppe , Gasparini Giorgio 
Published: April 2012

Spontaneous simultaneous bilateral quadriceps tendon rupture is associated with multiple medical conditions and pharmacological treatments; however, identifying prior risk factors is impossible in most cases. Achilles tendon and anterior cruciate ligament ruptures are associated with collagen, type V, alpha 1 (COL5A1) polymorphism. This genetic variant may be implicated quadriceps tendon rupture. The COL5A1 encodes the protein for pro-α1 chains of the low-abundance heterotrimeric type V collagen. In most noncartilaginous tissues, type V collagen is a quantitatively minor component of type I collagen that has been implicated in the regulation of the size and configuration of type I collagen fibrils. The functional significance of COL5A1 polymorphism in relation to type V collagen expression or activity has not been determined.
This article describes a patient with COL5A1 polymorphism and spontaneous simultaneous quadriceps tendon rupture. However, genetic and histologic studies performed on blood and tendon tissues and 3 consecutive sex- and age-matched controls showed a statistically significant reduction in collagen type V expression and an alteration in collagen structure in the tendon. These findings might explain the pathomechanisms of spontaneous tendon ruptures associated with COL5A1 polymorphism.
Acute Tibialis Posterior Tendon Rupture Associated With a Distal Tibial Fracture
Authors: Jarvis Hannah C. , Cannada Lisa K. 
Published: April 2012

Tibialis posterior tendon ruptures associated with closed medial malleolar fractures are rare. This article describes the association of tibialis posterior tendon ruptures with closed, high-energy, distal tibia fractures. Tendon ruptures are likely to be identified intraoperatively or missed if clinical evaluation at acute injury is limited. A high index of suspicion is required to diagnose this injury. The consequences of an unrecognized tibialis posterior tendon rupture include progressive, painful pes planus deformities due to the unopposed action of the peroneus brevis muscle and lack of support of the medial longitudinal arch. Secondary operative intervention may be required. This article describes an intraoperative tenodesis technique between the tibialis posterior and flexor digitorum longus tendons when direct repair is not possible.A 48-year-old woman sustained a closed AO/Orthopaedic Trauma Association type 43A right lower-extremity distal tibia fracture and a traumatic left knee arthrotomy. Temporary stabilization with an external fixator was performed, followed by open reduction and internal fixation of the distal tibial fracture 6 days later. A periarticular nonlocking medial plate was applied, and the tibialis posterior tendon was shortened. We performed a direct tenodesis to the flexor digitorum longus tendon. At 1-year follow-up, the patient had made excellent progress, with no detectable muscle weakness, and was able to perform a single-leg toe raise.A review of the literature suggested which features of radiological evidence of tendon rupture should be examined, which may be useful in the current era considering most high-energy distal tibia or pilon fractures undergo examination with computed tomography.
Intraosseous Median Nerve Entrapment Following Pediatric Posterior Elbow Dislocation
Authors: Bono Kenneth T. , Popp James E. 
Published: April 2012

Intra-articular entrapment of the median nerve following reduction of a pediatric posterior elbow dislocation is a rare complication but has been reported in the literature.
This article describes a case of a 7-year-old girl who sustained a posterior elbow dislocation associated with a medial epicondyle fracture and the subsequent intraosseous entrapment of her median nerve. The entrapment is believed to have resulted from new bone formation over the nerve that went unrecognized for nearly 2 years following injury. Routine imaging studies failed to detect the entrapment prior to exploratory surgery. Intra-articular entrapment of the median nerve must be suspected following pediatric elbow dislocation when concentric reduction fails, postreduction images demonstrate joint widening, or the patient has persistent clinical symptoms. This case demonstrates the potential for delay in diagnosis of the cause for neurological impairment following a relatively common injury in the pediatric population.
Objective intraoperative findings and intraoperative micropathology aided in limiting the amount of nerve resected to nonviable portions. Our case demonstrates the potential use of a cable nerve graft to bridge segmental defects in peripheral nerves.
Lower-extremity Soft Tissue Infections With Intra-abdominal Sources
Authors: Harrison Ryan K. , Wilmoth Jill C. , Griesser Michael J. , Granger Jeffrey F. 
Published: April 2012

This article describes a series of 3 patients who presented with lower-extremity soft tissue infections. Each patient was treated with prompt debridement by an orthopedic surgeon (J.F.G.) and required at least 1 additional procedure by another surgeon.
These infections vary from superficial cellulitis to rapidly advancing necrotizing fasciitis. At times, the source of these infections is clear. Other times, no obvious source of infection exists, in which case the abdomen must be considered as a possible source of infection. A high level of suspicion, complete history and physical examination, and appropriate ancillary studies are required to make an accurate and prompt diagnosis. Options for the treatment of the intra-abdominal source of infection depend on the etiology of the infection and anatomic location of the process. Psoas abscesses can often be decompressed by an interventional radiologist using computed tomography guidance. In the case of bowel involvement, such as suspected carcinoma or diverticulitis, a general surgeon is necessary. When the appropriate diagnosis is made, soft tissue infections of the thigh often respond to appropriate surgical debridement and antibiotic therapy. It is important to remember the whole patient when evaluating soft tissue infections, especially in the thigh. A low threshold for imaging of the abdomen and pelvis is important, especially when the physical examination or medical history reveals the abdomen as a possible source of infection.
Intraocular Lens Tilt and Decentration Measured By Scheimpflug Camera Following Manual or Femtosecond Laser–created Continuous Circular Capsulotomy
Authors: Kránitz Kinga , Miháltz Kata , Sándor Gábor L. , Takacs Agnes , Knorz Michael C. , Nagy Zoltán Z. 
Published: April 2012

PURPOSE:
To compare intraocular lens (IOL) decentration and tilt following a circular capsulotomy created with a femtosecond laser (laser CCC) to a manually performed continuous curvilinear capsulorrhexis (manual CCC).

METHODS:
In a prospective, randomized study, a laser CCC (Alcon LenSx Inc) was performed in 20 eyes from 20 patients and a manual CCC was performed in 25 eyes from 25 patients. Intraocular lens decentration and tilt were measured using a Scheimpflug camera (Pentacam, Oculus Optikgeräte GmbH) 1 year after surgery. Uncorrected (UDVA) and corrected distance visual acuity (CDVA) and manifest refraction were also determined postoperatively. Between-group differences of IOL decentration and tilt as well as the correlation between IOL decentration and postoperative refractive changes and between IOL tilt and visual acuity were analyzed.

RESULTS:

Horizontal and vertical tilt were significantly higher in the manual CCC group (
P
=.007 and
P
<.001, respectively). Lenses implanted after manual CCC showed greater horizontal and total decentration (
P
=.034 and
P
=.022, respectively). Significant differences were found in the homogeneity of dichotomized IOL vertical tilt and both horizontal and total decentration distribution (
P
=.008,
P
=.036, and
P
=.017, respectively). Total IOL decentration showed a significant correlation with changes in manifest refraction values between 1 month and 1 year after surgery (R=0.33,
P
=.032). A significant correlation was noted between IOL vertical tilt and CDVA (R
2
=0.17, β=−0.41, 95% confidence limit: −0.69 to −0.13,
P
=.005).

CONCLUSIONS:
Continuous curvilinear capsulorrhexis created with a femtosecond laser resulted in a more stable refractive result and less IOL tilt and decentration than manual CCC.
A Flexible and Qualitatively Stable Model for Cell Cycle Dynamics Including DNA Damage Effects
Authors: Jeffries Clark D. , Johnson Charles R. , Zhou Tong , Simpson Dennis A. , Kaufmann William K. 
Published: April 2012

This paper includes a conceptual framework for cell cycle modeling into which the experimenter can map observed data and evaluate mechanisms of cell cycle control. The basic model exhibits qualitative stability, meaning that regardless of magnitudes of system parameters its instances are guaranteed to be stable in the sense that all feasible trajectories converge to a certain trajectory. Qualitative stability can also be described by the signs of real parts of eigenvalues of the system matrix. On the biological side, the resulting model can be tuned to approximate experimental data pertaining to human fibroblast cell lines treated with ionizing radiation, with or without disabled DNA damage checkpoints. Together these properties validate a fundamental, first order systems view of cell dynamics. Classification Codes: 15A68
In Vitro Hemodynamic Evaluation of Ventricular Suction Conditions of the EVAHEART Ventricular Assist Pump
Authors: Ferreira Antonio L. , Yu Yih-Choung , Arnold Dorian K. , Vandenberghe Stijn , Grashow Jonathan , Kitano Tomoya , Borzelleca David , Antaki James F. 
Published: April 2012

Purpose: Mismatches between pump output and venous return in a continuous-flow ventricular assist device may elicit episodes of ventricular suction. This research describes a series of in vitro experiments to characterize the operating conditions under which the EVAHEART centrifugal blood pump (Sun Medical Technology Research Corp., Nagano, Japan) can be operated with minimal concern regarding left ventricular (LV) suction. Methods: The pump was interposed into a pneumatically driven pulsatile mock circulatory system (MCS) in the ventricular apex to aorta configuration. Under varying conditions of preload, afterload, and systolic pressure, the speed of the pump was increased step-wise until suction was observed. Identification of suction was based on pump inlet pressure. Results: In the case of reduced LV systolic pressure, reduced preload (≤10 mmHg), and afterload (≤60 mmHg), suction was observed for speeds ≥2,200 rpm. However, suction did not occur at any speed (up to a maximum speed of 2,400 rpm) when preload was kept within 10–14 mmHg and afterload ≥80 mmHg. Although in vitro experiments cannot replace in vivo models, the results indicated that ventricular suction can be avoided if sufficient preload and afterload are maintained. Conclusion: Conditions of hypovolemia and/or hypotension may increase the risk of suction at the highest speeds, irrespective of the native ventricular systolic pressure. However, in vitro guidelines are not directly transferrable to the clinical situation; therefore, patient-specific evaluation is recommended, which can be aided by ultrasonography at various points in the course of support.
A Comparison of Bicarbonate Kinetics and Acid-base Status in High Flux Hemodialysis and On-Line Post-Dilution Hemodiafiltration
Authors: Morel Hélène , Jaffrin Michel Y. , Lux Clément , Renou Marianne , Fessier Claire , Petit Abir , Morinière Philippe , Legallais Cécile 
Published: April 2012

Objectives: To compare bicarbonate kinetics and acid base status in HD and HDF for the same patient; and to investigate the effect of patient physiologic parameters on these kinetics. Methods: In order to monitor HCO3- kinetics during dialysis, acid-base parameters, pH, blood gases partial pressures, and HCO3- concentrations were recorded during 3 regular dialysis (HD) and 3 on-line post-dilution HDF sessions performed on 12 patients, using same dialysis fluid with a 38 mmol/l HCO3-concentration. HCO3- mass transfers through the hemodialyzers membranes and into the patients were continuously calculated during the sessions from HCO3- concentrations, together with HCO3-dialysance. The “apparent” HCO3-gain was calculated by integrating over time the instantaneous mass transfer from dialyzer and re-infusion fluid to the patient. A second method consisted in calculating the patient apparent bicarbonate space (ABS) and HCO3- mass (ABS times plasma concentration) at beginning and end of session. Results: No significant differences were observed between acid base parameters at the end of HD and HDF sessions. In contrast to urea clearances, HCO3- dialysances decayed with time during sessions from 110 to 140 ml/min to about 40 ml/min after one hour. The net HCO3- gain was taken as the difference between final and initial HCO3-masses. This net gain was in average 63% of apparent gain in HD and 74% in HDF. Conclusions: Uremic acidosis was well corrected without risk of alkalosis. An unexpected result was the continuous decay of bicarbonate dialysance both in HD and HDF during runs.
Extracorporeal Life Support: First Year of Experience Implementing the Technique in Slovenia
Authors: Golicnik Alenka , Knezevic Ivan , Racic Marija , Noc Marko , Gorjup Vojka 
Published: April 2012

Extracorporeal life support (ELS) is emerging as a standard treatment option for acute respiratory and/or cardiac failure. In this article we describe our first year of experience with ELS activity in adult medical patients in our center. Veno-venous extracorporeal membrane oxygenation (VV ECMO) support was applied in cases of severe acute respiratory distress syndrome (ARDS) not responsive to conventional treatments. The use of veno-arterial (VA) ECMO support was reserved for cases of cardiac shock refractory to standard treatment and cardiac arrests not responding to conventional resuscitation. A total of 19 patients were treated with ELS during the first year of activity. Eight of these received VV ECMO for ARDS of various etiologies, with a survival rate of 63%. Eleven patients received VA ECMO support due to cardiac failure (2 post-resuscitation). Survival in this group was 45%. We report our results, including complications and organizational issues that we encountered, and describe protocol improvements developed over the short period of time since ELS treatment has been implemented in our center.
Social marketing to plan a fall prevention program for latino construction workers
Authors: Menzel Nancy N. , Shrestha Pramen P. 
Published: April 2012

AbstractBackgroundLatino construction workers experience disparities in occupational death and injury rates. The Occupational Safety and Health Administration funded a fall prevention training program at the University of Nevada, Las Vegas in response to sharp increases in fall‐related accidents from 2005 to 2007. The grant's purpose was to improve fall protection for construction workers, with a focus on Latinos. This study assessed the effectiveness of social marketing for increasing fall prevention behaviors.MethodsA multi‐disciplinary team used a social marketing approach to plan the program. We conducted same day class evaluations and follow‐up interviews 8 weeks later.ResultsThe classes met trainee needs as evidenced by class evaluations and increased safety behaviors. However, Spanish‐speaking Latinos did not attend in the same proportion as their representation in the Las Vegas population.ConclusionsA social marketing approach to planning was helpful to customize the training to Latino worker needs. However, due to the limitations of behavior change strategies, future programs should target employers and their obligation to provide safer workplaces. Am. J. Ind. Med. 55:729–735, 2012. © 2012 Wiley Periodicals, Inc.
Prevalence and work‐relatedness of carpal tunnel syndrome in the working population, United States, 2010 national health interview survey
Authors: Luckhaupt Sara E. , Dahlhamer James M. , Ward Brian W. , Sweeney Marie H. , Sestito John P. , Calvert Geoffrey M. 
Published: April 2012

AbstractBackgroundPatterns of prevalence and work‐relatedness of carpal tunnel syndrome (CTS) among workers offer clues about risk factors and targets for prevention.MethodsData from an occupational health supplement to the 2010 National Health Interview Survey were used to estimate the prevalence of self‐reported clinician‐diagnosed CTS overall and by demographic characteristics. The proportion of these cases self‐reported to have been attributed to work by clinicians was also examined overall and by demographic characteristics. In addition, the distribution of industry and occupation (I&O) categories to which work‐related cases of CTS were attributed was compared to the distribution of I&O categories of employment among current/recent workers.ResultsData were available for 27,157 adults, including 17,524 current/recent workers. The overall lifetime prevalence of clinician‐diagnosed CTS among current/recent workers was 6.7%. The 12‐month prevalence was 3.1%, representing approximately 4.8 million workers with current CTS; 67.1% of these cases were attributed to work by clinicians, with overrepresentation of certain I&O categories.ConclusionsCTS affected almost 5 million U.S. workers in 2010, with prevalence varying by demographic characteristics and I&O. Am. J. Ind. Med. 56:615–624, 2013. © 2012 Wiley Periodicals, Inc.
Greenhouse gas emissions from the brazilian antarctic station “comandante ferraz”
Authors: Leripio Alexandre de Avila , Torres João Paulo Machado , Viana Mariana de Sá , Echelmeier Gustavo Rohden 
Published: April 2012

AbstractBackgroundClimate change is a natural phenomenon that has been intensified due to increased emissions of greenhouse gases (GHG). It has become an indispensable issue in international negotiations related to global sustainability and is deeply related to the overall status of environmental health in our planet.MethodsWe compiled an inventory of GHG emissions that resulted from human activities at the Brazilian Antarctic Scientific Station “Comandante Ferraz” (EACF) and collected emissions data relating to these activities from January to March 2011. The present work aims to identify the sources of GHG emissions, their characteristics, and composition, using as methodology and framework basis the international ISO 14,064:07 and the GHG Protocol.ResultsWe addressed emissions of CO2, CH4, and N2O arising from the use of vehicles, diesel–electric generators, boilers, and wastewater treatment for the sewage treatment plant. We identified that the main GHG emissions derived from the activities of power generation using diesel and boilers (more than 80% of the emissions), adding more than 772 t of CO2 equivalents.ConclusionsWe identified that the diesel generators and boilers are the most important sources of emission by Expedition XXIX (2010–2011). In that CO2 is principally emitted in relation to electrical energy generation from diesel generators, we emphasize the need for fuel burning reduction through energy consumption reduction. Am. J. Ind. Med. 55:1166–1171, 2012. © 2012 Wiley Periodicals, Inc.