bluuebunny/crossref_metadata_2025_split huggingface dataset:
Continuous elevation of PTH increases the number of osteoblasts via both osteoclast-dependent and -independent mechanisms
Authors: Jilka Robert L , O'Brien Charles A , Bartell Shoshana M , Weinstein Robert S , Manolagas Stavros C 
Published: June 2010

Abstract
Sustained parathyroid hormone (PTH) elevation stimulates bone remodeling (ie, both resorption and formation). The former results from increased RANKL synthesis, but the cause of the latter has not been established. Current hypotheses include release of osteoblastogenic factors from osteoclasts or from the bone matrix during resorption, modulation of the production and activity of osteoblastogenic factors from cells of the osteoblast lineage, and increased angiogenesis. To dissect the contribution of these mechanisms, 6-month-old Swiss-Webster mice were infused for 5 days with 470 ng/h PTH(1-84) or 525 ng/h soluble RANKL (sRANKL). Both agents increased osteoclasts and osteoblasts in vertebral cancellous bone, but the ratio of osteoblasts to osteoclasts and the increase in bone formation was greater in PTH-treated mice. Cancellous bone mass was maintained in mice receiving PTH but lost in mice receiving sRANKL, indicating that maintenance of balanced remodeling requires osteoblastogenic effects beyond those mediated by osteoclasts. Consistent with this contention, PTH, but not sRANKL, decreased the level of the Wnt antagonist sclerostin and increased the expression of the Wnt target genes Nkd2, Wisp1, and Twist1. Furthermore, PTH, but not sRANKL, increased the number of blood vessels in the bone marrow. Weekly injections of the RANKL antagonist osteoprotegerin at 10 µg/g for 2 weeks prior to PTH infusion eliminated osteoclasts and osteoblasts and prevented the PTH-induced increase in osteoclasts, osteoblasts, and blood vessels. These results indicate that PTH stimulates osteoclast-dependent as well as osteoclast-independent (Wnt signaling) pro-osteoblastogenic pathways, both of which are required for balanced focal bone remodeling in cancellous bone. © 2010 American Society for Bone and Mineral Research.
Bone density and hyperlipidemia: The T-lymphocyte connection
Authors: Graham Lucia S , Tintut Yin , Parhami Farhad , Kitchen Christina MR , Ivanov Yevgeniv , Tetradis Sotirios , Effros Rita B 
Published: June 2010

Abstract
Osteoporosis, which contributes to morbidity and mortality, often coexists with cardiovascular disease, especially atherosclerosis. We have reported recently that in vitro exposure of human T-lymphocytes to oxidized lipids induced expression of a key osteoclastogenic cytokine, receptor activator of NF-κB ligand (RANKL). Our previous studies have shown that mice fed an atherogenic high-fat diet developed osteopenia and that bone marrow preosteoclasts from these hyperlipidemic mice have increased osteoclastic potential. To investigate the role of T-lymphocytes in the diet-induced bone loss, C57BL/6 mice were fed either chow or a high-fat diet, and bone parameters and T-lymphocyte activation were assessed at 6 and 11 months. Consistent with our previous findings, peripheral quantitative computed tomographic (pQCT) analysis showed that mice in the high-fat group had lower bone mineral content than mice in the chow group. Furthermore, histomorphometric analysis showed decreased structural parameters in the high-fat group. Coculture studies showed that bone marrow cells isolated from the high-fat group, which contained increased levels of activated memory T-lymphocytes compared with bone marrow cells from the chow mice, supported osteoclastic differentiation of RAW 264.7 cells. Additionally, RANKL expression was upregulated significantly in the T-lymphocytes isolated from the bone marrow of the high-fat group. Splenic T-lymphocytes isolated from the high-fat group also had increased expression of transcripts for the receptor for oxidized lipids (LOX-1) as well as for inflammatory and osteoclastogenic cytokines, including RANKL, interleukin 6 (IL-6), tumor necrosis factor α (TNF-α), IL-1β, and interferon γ (IFN-γ). Together these findings suggest that T-lymphocytes play a key role in the osteoclastogenesis induced by a high-fat diet and may contribute to the bone loss associated with diet-induced osteopenia. © 2010 American Society for Bone and Mineral Research.
Visualizing of the cellular uptake and intracellular trafficking of exosomes by live‐cell microscopy
Authors: Tian Tian , Wang Yuanyuan , Wang Haitao , Zhu Zhaoqi , Xiao Zhongdang 
Published: June 2010

AbstractCells release exosomes to transfer various molecules to other cells. Exosomes are involved in a number of physiological and pathological processes. They are emerging great potential utility for diseases diagnosis and treatment recently. However, the internalization and intracellular trafficking of exosomes have not been described clearly. In this work, exosomes were isolated from the culture medium of PC12 cells, labeled by lipophilic dye and amino‐reactive fluorophore, incubated with resting PC12 cells. The results of live‐cell microscopy indicated that exosomes were internalized through endocytosis pathway, trapped in vesicles, and transported to perinuclear region. Particle tracking fluorescent vesicles suggested that the active transport of exosomes may be mediated by cytoskeleton. The proteins on exosome membrane were found to be released from exosomes and trapped in lysosome. The inverted transport of lipophilic dye from perinuclear region to cell peripheries was revealed, possibly caused by recycling of the exosome lipids. This study provides new sight into the mechanisms of exosome uptake and intracellular fate. J. Cell. Biochem. 111: 488–496, 2010. © 2010 Wiley‐Liss, Inc.
Culturally relevant family‐based treatment for adolescent delinquency and substance abuse: understanding within‐session processes
Authors: Cunningham Phillippe B. , Foster Sharon L. , Warner Sarah E. 
Published: June 2010

AbstractIdentifying psychotherapy processes that likely contribute to client outcome with ethnic minorities is a vital practice and research need, particularly within family‐focused, evidence‐based treatments (EBT) for youth with externalizing problems. Identifying process variables within a cross‐cultural context may improve the efficacy of EBTs by informing psychotherapists how to modify their behavior when working with ethnically diverse clients. The authors described one approach to the development of culturally competent psychotherapy, using an observational coding system comprising Afrocentric codes to investigate culturally relevant therapist behaviors. Qualitative examples illustrated the quantitative findings relating to therapist in‐session behavior that promote client engagement and positive responding during a midtreatment session of multisystemic therapy. © 2010 Wiley Periodicals, Inc. J Clin Psychol: In Session 66:1–17, 2010.
Impact of KCNE subunits on KCNQ1 (Kv7.1) channel membrane surface targeting
Authors: Roura‐Ferrer Meritxell , Solé Laura , Oliveras Anna , Dahan Rinat , Bielanska Joanna , Villarroel Álvaro , Comes Núria , Felipe Antonio 
Published: June 2010

AbstractThe KCNQ1 (Kv7.1) channel plays an important role in cardiovascular physiology. Cardiomyocytes co‐express KCNQ1 with KCNE1‐5 proteins. KCNQ1 may co‐associate with multiple KCNE regulatory subunits to generate different biophysically and pharmacologically distinct channels. Increasing evidence indicates that the location and targeting of channels are important determinants of their function. In this context, the presence of K+ channels in sphingolipid–cholesterol‐enriched membrane microdomains (lipid rafts) is under investigation. Lipid rafts are important for cardiovascular functioning. We aimed to determine whether KCNE subunits modify the localization and targeting of KCNQ1 channels in lipid rafts microdomains. HEK‐293 cells were transiently transfected with KCNQ1 and KCNE1–5, and their traffic and presence in lipid rafts were analyzed. Only KCNQ1 and KCNE3, when expressed alone, co‐localized in raft fractions. In addition, while KCNE2 and KCNE5 notably stained the cell surface, KCNQ1 and the rest of the KCNEs showed strong intracellular retention. KCNQ1 targets multiple membrane surface microdomains upon association with KCNE peptides. Thus, while KCNQ1/KCNE1 and KCNQ1/KCNE2 channels target lipid rafts, KCNQ1 associated with KCNE3–5 did not. Channel membrane dynamics, analyzed by fluorescence recovery after photobleaching (FRAP) experiments, further supported these results. In conclusion, the trafficking and targeting pattern of KCNQ1 can be influenced by its association with KCNEs. Since KCNQ1 is crucial for cardiovascular physiology, the temporal and spatial regulations that different KCNE subunits may confer to the channels could have a dramatic impact on membrane electrical activity and putative endocrine regulation. J. Cell. Physiol. 225: 692–700, 2010. © 2010 Wiley‐Liss, Inc.
Calcitonin gene‐related peptide (CGRP) inhibits apoptosis in human osteoblasts by β‐catenin stabilization
Authors: Mrak Emanuela , Guidobono Francesca , Moro Gianluigi , Fraschini Gianfranco , Rubinacci Alessandro , Villa Isabella 
Published: June 2010

AbstractTransgenic mice over‐expressing calcitonin gene‐related peptide (CGRP) in osteoblasts have increased bone density due to increased bone formation, thus suggesting that CGRP plays a role in bone metabolism. In this study we determined the relationship between CGRP, the canonical Wnt signaling and apoptosis in human osteoblasts (hOBs) in consideration of the well‐documented involvement of this pathway in bone cells. Primary cultures of hOBs were treated with CGRP 10−8 M. Levels of β‐catenin, which is the cytoplasmic protein mediator of canonical Wnt signaling, and mRNA were determined. CGRP increases both the expression and the levels of cytoplasmic β‐catenin by binding to its receptor, as this effect is blocked by the antagonist CGRP8–37. This facilitatory action on β‐catenin appears to be mediated by the inhibition of the enzyme GSK‐3β via protein kinase A (PKA) activation. GSK‐3β is a glycogen synthase kinase that, by phosphorylating β‐catenin, promotes its degradation by the proteosomal machinery. Moreover, the peptide is able to inhibit hOBs apoptosis stimulated by dexamethasone or by serum deprivation, possibly through the accumulation of β‐catenin, since the inhibitor of PKA activity H89 partially prevents the antiapoptotic effect of the peptide. In conclusion CGRP, released by nerve fibers, exerts its anabolic action on bone cells by stimulating canonical Wnt signaling and by inhibiting hOBs apoptosis, thus favoring local bone regeneration. J. Cell. Physiol. 225: 701–708, 2010. © 2010 Wiley‐Liss, Inc.
Activin A and bone metastasis
Authors: Leto Gaetano 
Published: June 2010

AbstractActivin A, is a multifunctional cytokine of the transforming growth factor‐β superfamily of growth factors. This molecule has been shown to be implicated in the regulation of a broad range of important biological functions including bone remodelling. Therefore, a deregulation in the activin signalling pathway may result in disturbances of normal bone metabolism and, eventually, in the onset of severe pathological conditions associated with an altered bone resorption. These observations support the concept that Act A might also be implicated in the pathogenesis of bone metastasis. This review provides insight into the most recent advances in understanding the role of this growth factor in the pathogenesis of bone metastasis, and discusses the implications related to the biomedical applications of these findings. J. Cell. Physiol. 225: 302–309, 2010. © 2010 Wiley‐Liss, Inc.
c‐Jun N‐terminal kinase is largely involved in the regulation of tricellular tight junctions via tricellulin in human pancreatic duct epithelial cells
Authors: Kojima Takashi , Fuchimoto Jun , Yamaguchi Hiroshi , Ito Tatsuya , Takasawa Akira , Ninomiya Takafumi , Kikuchi Shin , Ogasawara Noriko , Ohkuni Tsuyoshi , Masaki Tomoyuki , Hirata Koichi , Himi Tetsuo , Sawada Norimasa 
Published: June 2010

AbstractTricellulin (TRIC) is a tight junction protein at tricellular contacts where three epithelial cells meet, and it is required for the maintenance of the epithelial barrier. To investigate whether TRIC is regulated via a c‐Jun N‐terminal kinase (JNK) pathway, human pancreatic HPAC cells, highly expressed at tricellular contacts, were exposed to various stimuli such as the JNK activators anisomycin and 12‐O‐tetradecanoylphorbol 13‐acetate (TPA), and the proinflammatory cytokines IL‐1β, TNFα, and IL‐1α. TRIC expression and the barrier function were moderated by treatment with the JNK activator anisomycin, and suppressed not only by inhibitors of JNK and PKC but also by siRNAs of TRIC. TRIC expression was induced by treatment with the PKC activator TPA and proinflammatory cytokines IL‐1β, TNFα, and IL‐1α, whereas the changes were inhibited by a JNK inhibitor. Furthermore, in normal human pancreatic duct epithelial cells using hTERT‐transfected primary cultured cells, the responses of TRIC expression to the various stimuli were similar to those in HPAC cells. TRIC expression in tricellular tight junctions is strongly regulated together with the barrier function via the JNK transduction pathway. These findings suggest that JNK may be involved in the regulation of tricellular tight junctions including TRIC expression and the barrier function during normal remodeling of epithelial cells, and prevent disruption of the epithelial barrier in inflammation and other disorders in pancreatic duct epithelial cells. J. Cell. Physiol. 225: 720–733, 2010. © 2010 Wiley‐Liss, Inc.
Ilim Pulp, blending former Russian state enterprises, creates a corporate university to change culture, become an industry leader
Authors: Latuha Marina O. 
Published: June 2010

AbstractThis article describes the establishment of a corporate university in a Russian company. Following the economic restructuring of the country, training and personnel development became vital ingredients in the company's long‐term strategy. In these turbulent conditions, the company realized that it needed to have the training and personnel development characterized by continuity on the one hand and revolutionary changes in organizational culture on the other. If this could be achieved, it would encourage entrepreneurship, innovation, and change in the internal processes of the organization. The case describes the stages, programs, and basic components of the corporate university model. It not only illustrates the basic issues in the application of corporate training theory, but also analyzes the risks and problems for the company in the project's realization. The article concludes with a description of how the corporate university developed after its initiation, and some conclusions about the overall success of the project. © 2010 Wiley Periodicals, Inc.
The profile of a successful midlevel manager at AstraZeneca proves that great leaders aren't only in the c‐suite
Authors: Hurd Jean L. 
Published: June 2010

AbstractThe company's wise investment in a high‐potential leader has paid big dividends for the organizations she has led. Rose Ann Scanlon's success in getting breakthrough business results is due to a leadership style rooted in an unshakeable belief in the power of fully engaged employees and the responsibility of their leaders to be an enabling influence every step of the way. The author traces Rose Ann's journey from lawyer to midlevel manager, and then draws on feedback from bosses, direct reports, and peers to characterize the aspects of Rose Ann's leadership approach that make her so effective in taking her organizations to new levels of performance, and in grooming other transformational leaders. © 2010 Wiley Periodicals, Inc.
Raman spectroscopy of hydrogen arsenate group (AsO3OH)2− in solid‐state compounds: cobalt‐containing zinc arsenate mineral, koritnigite (Zn,Co)(AsO3OH)·H2O
Authors: Frost Ray L. , Sejkora Jiří , Čejka Jiří , Plášil Jakub , Bahfenne Silmarilly , Keeffe Elloise C. 
Published: June 2010

AbstractRaman spectra of two well‐defined types of koritnigite crystals from the Jáchymov ore district, Czech Republic, were recorded and interpreted. No substantial differences were observed between both crystal types. The observed Raman bands were attributed to the (AsO3OH)2− stretching and bending vibrations as well as stretching and bending vibrations of water molecules and hydroxyl ions. The non‐interpreted Raman spectra of koritnigite from the RRUFF database and the published infrared spectra of cobaltkoritnigite were used for comparison. The OH···O hydrogen bond lengths in the crystal structure of koritnigite were inferred from the Raman spectra and compared with those derived from the X‐ray single‐crystal refinement. The presence of (AsO3OH)2− units in the crystal structure of koritnigite was proved from the Raman spectra, which supports the conclusions of the X‐ray structure analysis. Copyright © 2010 John Wiley & Sons, Ltd.
Fractionation of phosphopeptides on strong anion‐exchange capillary trap column for large‐scale phosphoproteome analysis of microgram samples
Authors: Wang Fangjun , Han Guanghui , Yu Zhiyuan , Jiang Xinning , Sun Shutao , Chen Rui , Ye Mingliang , Zou Hanfa 
Published: June 2010

AbstractIt is one of the key issues to develop powerful fractionating method to increase the identification of the low‐abundance phosphopeptides. In this study, a semi‐online 2‐D LC separation strategy based on three‐step fractionation of the enriched peptides on strong anion‐exchange trap column was developed. It was demonstrated that the sensitivity and phosphoproteome coverage obtained by this fractionating method with strong anion‐exchange trap column is much higher than those by the conventional methods based on C18 trap column. In addition, when the same amount of sample was loaded, the number of identified phosphopeptides had increased 108%. Combination of this three‐step fractionation method with RPLC‐MS/MS analysis by 300 min RP‐gradient separation was applied to phosphoproteome analysis of human liver proteins, and 853 unique phosphopeptides was positively identified from 500 μg tryptic digest of human liver proteins. After three cycles' consecutive analyses, 1554 unique phosphopeptides and 1566 phosphorylated sites were totally identified from 735 phosphorylated proteins at a false discovery rate of <1% in about 54 h of analysis time.
Liquid chromatography‐tandem mass spectrometric method for determination of E121 in mouse plasma and its application to pharmacokinetics
Authors: Matar Kamal M. , El‐Hashim Ahmed Z. , Edafiogho Ivan O. 
Published: June 2010

AbstractA rapid LC‐MS/MS method for quantification of an enaminone analog, E121 in mouse plasma using E118 as an internal standard (IS) has been developed and validated. The analyte was analyzed on C18 column using a mobile phase of acetonitrile/methanol/ammonium acetate/formic acid (60:20:20:0.025, v/v/v/v) at a flow rate of 0.25 mL/min. Quantitation was achieved using ESI+ interface, employing MRM mode at m/z 308>262 and 222>194 for E121 and IS, respectively. The calibration standards were linear over a range of 0.10–20 μg/mL (r2>0.99) with an LLOQ of 0.1 μg/mL (RSD%; 11.4% and bias%; 9.5%). Intra‐ and inter‐run precision of E121 assay ranged from 3.7 to 10.9% with accuracy (bias) that varied between −10.0 and 12.0%, demonstrating good precision and accuracy. Recoveries of E121 and the IS from plasma were above 80%. Stability of E121 in plasma showed that the analyte was stable under various conditions. The matrix effect study showed a lack of effect. The applicability of the developed method was demonstrated by measuring E121 in mouse plasma samples following intraperitoneal administration of various doses ranging from 10 to 100 mg/kg and this study demonstrates that E121 exhibits linear kinetics in the dose range studied.
Simultaneous determination of yohimbine, sildenafil, vardenafil and tadalafil in dietary supplements using high‐performance liquid chromatography‐tandem mass spectrometry
Authors: Zhang Ying , Huang Zhiqiang , Ding Li , Yan Hongfei , Wang Meiling , Zhu Shaohua 
Published: June 2010

AbstractA simple and sensitive method was developed for determination of illegal adulterants (yohimbine, sildenafil, vardenafil and tadalafil) in dietary supplements by HPLC‐MS/MS. The separation was achieved on a C18 column with the mobile phase consisting of acetonitrile and 0.1% acetic acid aqueous solution with a gradient elution at a flow rate of 0.5 mL/min. The analytes were quantified and identified by two characteristic transitions using the multiple‐reaction monitoring mode. The recoveries of the analytes ranged from 77.5 to 109.3% with the RSD less than 8.1% (n=6). The method has been successfully applied to screen illegal adulterations of natural dietary supplements.
Development and validation of an ultra high‐performance liquid chromatographic method for the determination of a diastereomeric impurity in (+)‐pinoresinol diglucoside chemical reference substance
Authors: Song Jing‐Zheng , Cheung Lok Man , Liu Xin , Qiao Chun‐Feng , Zhou Yan , Li Song‐Lin , Chen Shi‐Lin , Xu Hong‐Xi 
Published: June 2010

Abstract(+)‐Pinoresinol 4,4′‐di‐O‐β‐D‐glucopyranoside ((+)‐PDG) is one of the major lignans with various pharmacological activities which could be isolated from Duzhong and other plant species. In this study, a diastereomeric impurity, (−)‐pinoresinol 4,4′‐di‐O‐β‐D‐glucopyranoside ((−)‐PDG), the main impurity was identified in (+)‐PDG chemical reference substance (CRS) and a reliable chromatographic method for rapid purity determination of (+)‐PDG CRS was firstly developed. The optimal chromatographic condition was found to be using ACN/1,4‐dioxane–water (2.5:6:91.5, v/v/v) as mobile phase on a Waters Acquity UPLC HSS T3 column (2.1 mm×100 mm, 1.8 μm) with column temperature of 37°C. The method was validated and applied to determine the chromatographic purity of five (+)‐PDG CRS samples. The content of (−)‐PDG in four commercial (+)‐PDG CRS was 8.47–20.30%, whereas no (−)‐PDG was detected in our in‐house prepared (+)‐PDG CRS in which purity was confirmed to be 99.80%. The above results confirmed that this method is fast and highly efficient for purity determination of the (+)‐PDG CRS.
Development of magnetic octadecylsilane particles as solid‐phase extraction adsorbent for the determination of fat‐soluble vitamins in fruit juice‐milk beverage by capillary liquid chromatography
Authors: Hu Chaofan , Jia Li , Liu Qingqing , Zhang Sheng 
Published: June 2010

AbstractA sensitive, fast and simple method based on magnetic octadecylsilane particles was developed for the extraction of three fat‐soluble vitamins followed by capillary LC (CLC) analysis with UV detection. Magnetic octadecylsilane particles were prepared based on three‐step reactions including co‐precipitation, sol‐gel polymerization and alkylation. The characterization of the prepared product was studied by scanning electron microscope and Fourier‐transform infrared spectrometry. The particles were used as magnetic SPE adsorbent for the extraction of fat‐soluble vitamins in fruit juice‐milk beverage. The extraction condition and efficiency of the particles for fat‐soluble vitamins were investigated. By coupling magnetic SPE with capillary LC with UV detection, low concentrations of fat‐soluble vitamins in fruit juice‐milk beverage can be detected without the interference from other substances in the sample matrix.