bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reactivation of p53 mutants by p53 reactivation and induction of massive apoptosis in thyroid cancer cells
Authors: Messina Rosa Linda , Sanfilippo Mariangela , Vella Veronica , Pandini Giuseppe , Vigneri Paolo , Nicolosi Maria Luisa , Gianì Fiorenza , Vigneri Riccardo , Frasca Francesco 
Published: June 2011

AbstractMost undifferentiated thyroid carcinomas express p53 mutants and thereafter, are very resistant to chemotherapy. p53 reactivation and induction of massive apoptosis (Prima‐1) is a compound restoring the tumor‐suppressor activity of p53 mutants. We tested the effect of Prima‐1 in thyroid cancer cells harboring p53 mutations. Increasing doses of Prima‐1 reduced viability of thyroid cancer cells at a variable extent (range 20–80%). Prima‐1 up‐regulated p53 target genes (p21WAF1, BCL2‐associated X protein (Bax), and murine double minute 2 (MDM2)), in BC‐PAP and Hth‐74 cells (expressing D259Y/K286E and K286E p53 mutants) but had no effect in SW1736 (p53 null) and TPC‐1 (expressing wild‐type p53) thyroid cancer cells. Prima‐1 also increased the cytotoxic effects of either doxorubicin or cisplatin in thyroid cancer cells, including the chemo‐resistant 8305C, Hth‐74 and BC‐PAP cells. Moreover, real‐time PCR and Western blot indicated that Prima‐1 increases the mRNA of thyroid‐specific differentiation markers in thyroid cancer cells. Fluorescence‐activated cell sorting analysis revealed that Prima‐1 effect on thyroid cancer cells occurs via the enhancement of both cell cycle arrest and apoptosis. Small interfering RNA experiments indicated that Prima‐1 effect is mediated by p53 mutants but not by the p53 paralog p73. Moreover, in C‐643 thyroid cancer cells, forced to ectopically express wild‐type p53, Prima‐1 prevented the dominant negative effect of double K248Q/K286E p53 mutant. Finally, co‐IP experiments indicated that in Hth‐74 cells Prima‐1 prevents the ability of p53 mutants to sequestrate the p53 paralog TAp73. These in vitro studies imply that p53 mutant reactivation by small compounds may become a novel anticancer therapy in undifferentiated thyroid carcinomas.
Pleural malignant mesothelioma epidemic: Incidence, modalities of asbestos exposure and occupations involved from the Italian National Register
Authors: Marinaccio Alessandro , Binazzi Alessandra , Marzio Davide Di , Scarselli Alberto , Verardo Marina , Mirabelli Dario , Gennaro Valerio , Mensi Carolina , Riboldi Luciano , Merler Enzo , Zotti Renata De , Romanelli Antonio , Chellini Elisabetta , Silvestri Stefano , Pascucci Cristiana , Romeo Elisa , Menegozzo Simona , Musti Marina , Cavone Domenica , Cauzillo Gabriella , Tumino Rosario , Nicita Carmela , Melis Massimo , Iavicoli Sergio ,   ReNaM Working Group
Published: June 2011

AbstractDue to the large scale use of asbestos (more than 3.5 million tons produced or imported until its definitive banning in 1992), a specific national surveillance system of mesothelioma incident cases is active in Italy, with direct and individual anamnestic etiological investigation. In the period between 1993 and 2004, a case‐list of 8,868 pleural MM was recorded by the Italian National Register (ReNaM) and the modalities of exposure to asbestos fibres have been investigated for 6,603 of them. Standardized incidence rates are 3.49 (per 100,000 inhabitants) for men and 1.25 for women, with a wide regional variability. Occupational asbestos exposure was in 69.3% of interviewed subjects (N = 4,577 cases), while 4.4% was due to cohabitation with someone (generally, the husband) occupationally exposed, 4.7% by environmental exposure from living near a contamination source and 1.6% during a leisure activity. In the male group, 81.5% of interviewed subjects exhibit an occupational exposure. In the exposed workers, the median year of first exposure was 1957, and mean latency was 43.7 years. The analysis of exposures by industrial sector focuses on a decreasing trend for those traditionally signaled as “at risk” (asbestos‐cement industry, shipbuilding and repair and railway carriages maintenance) and an increasing trend for the building construction sector. The systematic mesothelioma surveillance system is relevant for the prevention of the disease and for supporting an efficient compensation system. The existing experience on all‐too‐predictable asbestos effects should be transferred to developing countries where asbestos use is spreading.
Portland cement for bone tissue engineering: Effects of processing and metakaolin blends
Authors: Gallego‐Perez Daniel , Higuita‐Castro Natalia , Quiroz Felipe García , Posada Olga M. , López Luis E. , Litsky Alan S. , Hansford Derek J. 
Published: June 2011

AbstractThe need for a suitable scaffolding material for load bearing bone tissue engineering still has yet to be met satisfactorily. In this study, Portland cement and Portland cement/metakaolin (MK) blends were processed to render them biologically and mechanically suitable for such application. Portland cement was mixed with MK at different ratios. The slurries were hydrated under atmospheric (noncarbonated samples) and high‐CO2 conditions (carbonated samples). The mechanical properties were characterized via compressive tests. The bioactivity was analyzed in a simulated body fluid solution. Scanning electron microscopy and energy dispersive spectroscopy were used to evaluate sample morphology and chemistry. The cytocompatibility (direct contact assay, MTT test, and alkaline phosphatase activity) was tested using human osteoblast‐like cells. Cell responses were observed via conventional and electron microscopy. The results showed that the implementation of MK did not significantly influence the mechanical properties. All the samples evidenced bioactive behavior. Cell experiments confirmed a highly cytotoxic response to the noncarbonated specimens. The introduction of MK as well as the CO2 pretreatment significantly improved the cytocompatibility of the specimens. These results show that properly processed Portland cement and Portland cement/MK blends could present suitable properties for the development of load‐bearing scaffolding structures in bone tissue‐engineering applications. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.
Complement activation by candidate biomaterials of an implantable microfabricated medical device
Authors: Sokolov Andrey , Hellerud Bernt C. , Pharo Anne , Johannessen Erik A. , Mollnes Tom E. 
Published: June 2011

AbstractImplantable devices realized by microfabrication have introduced a new class of potential biomaterials whose properties would need to be assessed. Such devices include sensors for measuring biological substances like glucose. Thus, 14 different candidate materials intended for design of such a device were investigated with respect to their complement activation potential in human serum. The fluid‐phase activation was measured by the products C4d, Bb, C3bc, and the terminal complement complex (TCC), whereas solid‐phase activation was measured by deposition of TCC on the material surfaces. No fluid‐phase activation was found for materials related to the capsule, carrier, or sealing. Fluid‐phase activation was, however, triggered to a various extent in three of the four nanoporous membranes (cellulose, polyamide, and aluminium oxide), whereas polycarbonate was rendered inactive. Solid‐phase activation discriminated more sensitively between all the materials, revealing that the capsule candidate polydimethylsiloxane and sealing candidate silicone 3140 were highly compatible, showing significantly lower TCC deposition than the negative control (p < 0.01). Three of the candidate materials were indifferent, whereas the remaining nine showed significantly higher deposition of TCC than the negative control (p < 0.01). In conclusion, complement activation, in particular when examined on the solid phase, discriminated well between the different candidate materials tested and could be used as a guide for the selection of the best‐suited materials for further investigation and development of the device. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.
Mindfulness as a moderator in expressive writing
Authors: Poon Alvin , Danoff‐Burg Sharon 
Published: June 2011

AbstractRandomized, controlled studies have documented positive physical and psychological effects of writing about traumatic stress. Some of these studies have shown that individual differences play an important role, with participants responding differently to the intervention based on their personal characteristics. In the present expressive writing experiment, the trait of mindfulness was examined as a potential moderator. Seventy‐six undergraduates were randomly assigned to either expressive writing (n = 37) or a control group (n = 39). Main effects favoring expressive writing were found, and these were qualified by significant interactions with mindfulness. Specifically, individuals with higher mindfulness scores responded better to expressive writing, experiencing greater physical and psychological benefits than individuals with lower mindfulness scores. © 2011 Wiley Periodicals, Inc. J Clin Psychol 67:1–15, 2011.
Pain and emotion: a biopsychosocial review of recent research
Authors: Lumley Mark A. , Cohen Jay L. , Borszcz George S. , Cano Annmarie , Radcliffe Alison M. , Porter Laura S. , Schubiner Howard , Keefe Francis J. 
Published: June 2011

AbstractObjective and Method:Research on emotion and pain has burgeoned. We review the last decade's literature, focusing on links between emotional processes and persistent pain.Results:Neurobiological research documents the neural processes that distinguish affective from sensory pain dimensions, link emotion and pain, and generate central nervous system pain sensitization. Psychological research demonstrates that greater pain is related to emotional stress and limited emotional awareness, expression, and processing. Social research shows the potential importance of emotional communication, empathy, attachment, and rejection.Conclusions:Emotions are integral to the conceptualization, assessment, and treatment of persistent pain. Research should clarify when to eliminate or attenuate negative emotions, and when to access, experience, and express them. Theory and practice should integrate emotion into cognitive‐behavioral models of persistent pain. © 2011 Wiley Periodicals, Inc. J Clin Psychol 67:1–27, 2011.
Differential effect of oxidative or excitotoxic stress on the transcriptional profile of amyotrophic lateral sclerosis‐linked mutant SOD1 cultured neurons
Authors: Boutahar Nadia , Wierinckx Anne , Camdessanche Jean Philippe , Antoine Jean‐Christophe , Reynaud Evelyne , Lassabliere François , Lachuer Joël , Borg Jacques 
Published: June 2011

AbstractAmyotrophic lateral sclerosis (ALS) is a progressive, lethal, degenerative disorder of motor neurons. The causes of most cases of ALS are as yet undefined. In a previous study, it was shown that N‐methyl‐D‐aspartate (NMDA) and H2O2 stimuli reduce neuronal survival in cortical neurons in culture (Boutahar et al., 2008). To identify variations in gene expression in response to these neurotoxins in transgenic vs. control cortical neurons cultures, both microarray and RT‐PCR analysis were performed. High‐density oligonucleotide microarrays showed changes in the expression of about 600 genes involved in protein degradation, neurotrophic factors pathway, cell cycle, inflammation, cytoskeleton, cell adhesion, transcription, or signalling. The most up‐regulated genes following H2O2 treatment were involved in cytoskeletal organization and axonal transport, such as ARAP2, KIF17, and DKK2, or in trophic factors pathways, such as insulin‐like growth factor‐binding protein 4 (IGFBP4), FGF17, and serpin2. The most down‐regulated genes were involved in ion transport, such as TRPV1. After NMDA treatment, the most up‐regulated genes were involved in protein degradation, such as ubiquitin‐conjugating enzyme E2I and cathepsin H, and the most down‐regulated genes were involved in ion transport, such as SCN7A. We conclude that these neurotoxins act through different transcriptional inductions, and these changes may reflect an adaptative cellular response to the cellular stress induced by the neurotoxins involved in ALS in the presence of mutant human SOD1. © 2011 Wiley‐Liss, Inc.
ERK1/2 and ERK5 have distinct roles in the regulation of brain‐derived neurotrophic factor expression
Authors: Su Chang , Underwood Wendy , Rybalchenko Nataliya , Singh Meharvan 
Published: June 2011

AbstractNeurotrophins play essential roles in the development, differentiation, and survival of neuronal and nonneuronal cells. Alterations in neurotrophin expression have been implicated in a variety of neurodegenerative disorders. Dysregulation of brain‐derived neurotrophic factor (BDNF) has been implicated in deficits of long‐term potentiation and cognition and may contribute to the development of Alzheimer's disease (AD). In this study, we used complementary pharmacological and molecular approaches to evaluate the role of ERK1/2 and ERK5, two members of the MAPK pathway associated with neuroprotection, in regulating BDNF expression in C6 glial cells and primary astrocytes. Our data revealed that U0126, an inhibitor of both ERK5 and ERK1/2, increased the levels of BDNF mRNA, whereas the MEK1/2‐specific inhibitor PD184352 did not, suggesting that ERK5 exerts negative control over BDNF expression. This was supported by experiments in which RNAi‐mediated depletion of ERK5 led to an increase in BDNF. In contrast, transfection with constitutively active MEK5 resulted in an inhibition of BDNF expression, confirming the inhibitory role of ERK5 in the regulation of BDNF. Interestingly, transfection with the dominant active mutant of MEK1 (MEKR4F), the upstream activator of ERK1/2, resulted in a modest increase in BDNF levels. Collectively, our data suggest that ERK5 and ERK1/2 exert opposite effects on BDNF expression and support the hypothesis that an imbalance of these two signaling pathways may contribute to the pathology of diseases in which neurotrophin dysregulation is noted. © 2011 Wiley‐Liss, Inc.
Lubricin distribution in the torn human anterior cruciate ligament and meniscus
Authors: Zhang Dafang , Cheriyan Thomas , Martin Scott D. , Gomoll Andreas H. , Schmid Thomas M. , Spector Myron 
Published: June 2011

AbstractThe objective of this study was to: (1) determine the distribution of lubricin in the human torn anterior cruciate ligament (ACL) and meniscus; (2) determine the distribution of lubricin in the human intact ACL and meniscus; (3) and identify potential cellular sources of lubricin in these tissues. Ten torn ACLs and six torn menisci were obtained from surgeries; for comparison, 11 intact ACLs and 13 intact menisci were obtained from total knee replacements. Samples were formalin fixed and processed for immunohistochemical staining with a monoclonal antibody for lubricin. In torn ACLs and menisci, lubricin was generally found as a discrete layer covering the torn surface. No surface lubricin staining was found on the transected edges produced during excision. Lubricin was also found on the native surfaces of intact ACLs and menisci. In all tissues, lubricin was found in the matrix and intracellularly. The surface layer of lubricin coating torn edges of ACLs and menisci may interfere with the integrative healing process needed for repair. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29:1916–1922, 2011
Monoliths with immobilized zirconium ions for selective enrichment of phosphopeptides
Authors: Wang Hui , Duan Jicheng , Xu Hongjiu , Zhao Liang , Liang Yu , Shan Yichu , Zhang Lihua , Liang Zhen , Zhang Yukui 
Published: June 2011

AbstractTo meet the demands of protein phosphorylation study, immobilized zirconium ion affinity chromatography (Zr4+‐IMAC) monolith was prepared by combining UV‐initiated polymerization of monolithic support and subsequent photografting in both capillary columns and microchannels. Hydrophilic poly(2‐hydroxyethyl methacrylate (HEMA)‐co‐ethylene dimethacrylate (EDMA)) monolithic support was prepared under UV irradiation at the wavelength of 365 nm with monomer HEMA, crosslinker EDMA and 2,2‐dimethoxy‐2‐phenylacetophenone as photoinitiator in 1‐decanol solution, which provides good biocompatibility and permeability for biomolecule analysis. To introduce chelating ligands, such as phosphate groups, on the pore surface of monolith for metal ion immobilization, photografting of ethylene glycol methacrylate phosphate with benzophenone as the photoinitiator was performed at 254 nm for 300 s. The grafting process and metal ion immobilization can be monitored by measuring the electroosmotic flow produced by the modified monolith, providing a quantitative evaluation of post‐modification. This new method for the preparation of Zr4+‐IMAC monolith simplifies the optimization of monolith preparation and avoids the time‐consuming chemical modification process. Additionally, advantages include facile preparation in microdevices, easy regenerability and good reproducibility. After optimization, the microchip‐based Zr4+‐IMAC monolith was used for phosphopeptide analysis and showed good selectivity in phosphopeptide enrichment with matrix‐assisted laser desorption ionization mass spectrometry detection.
Habitat suitability and nest survival of white‐headed woodpeckers in unburned forests of Oregon
Authors: Hollenbeck Jeff P. , Saab Victoria A. , Frenzel Richard W. 
Published: June 2011

AbstractWe evaluated habitat suitability and nest survival of breeding white‐headed woodpeckers (Picoides albolarvatus) in unburned forests of central Oregon, USA. Daily nest‐survival rate was positively related to maximum daily temperature during the nest interval and to density of large‐diameter trees surrounding the nest tree. We developed a niche‐based habitat suitability model (partitioned Mahalanobis distance) for nesting white‐headed woodpeckers using remotely sensed data. Along with low elevation, high density of large trees, and low slope, our habitat suitability model suggested that interspersion–juxtaposition of low‐ and high‐canopy cover ponderosa pine (Pinus ponderosa) patches was important for nest‐site suitability. Cross‐validation suggested the model performed adequately for management planning at a scale >1 ha. Evaluation of mapped habitat suitability index (HSI) suggested that the maximum predictive gain (HSI = 0.36), where the number of nest locations are maximized in the smallest proportion of the modeled landscape, provided an objective initial threshold for identification of suitable habitat. However, managers can choose the threshold HSI most appropriate for their purposes (e.g., locating regions of low–moderate suitability that have potential for habitat restoration). Consequently, our habitat suitability model may be useful for managing dry coniferous forests for white‐headed woodpeckers in central Oregon; however, model validation is necessary before our model could be applied to other locations. © 2011 The Wildlife Society.
Cochlear reimplantation with same device: Surgical and audiologic results
Authors: van der Marel Kim S. , Briaire Jeroen J. , Verbist Berit M. , Joemai Raoul M.S. , Boermans Peter‐Paul B.M. , Peek Ferdinand A.W. , Frijns Johan H.M. 
Published: June 2011

AbstractObjectives/Hypothesis:To study to what extent it is possible to achieve identical insertion depths and to maintain the same performance after cochlear reimplantation.Study Design:Outcome research on a retrospective case series in a tertiary university referral center.Methods:Data were collected for 12 adults and three children who underwent reimplantation during the last 3 years with a new HiRes90K device with HiFocus 1J electrode owing to failure of the feed‐through seal. Multislice computed tomography scans were used to compare positions of the original and newly placed electrode arrays. The speech‐perception scores on a consonant‐vowel‐consonant word test before and after reimplantation were compared.Results:All reimplantations were successfully performed by two experienced cochlear implantation surgeons, and no complications were observed. Postoperative imaging showed that the average displacement of the new implant was only 0.59 mm. Reactivation of the implant gave immediate open set speech understanding in all patients, and speech perception rapidly returned to the previous level obtained with the original implant within weeks; it was even significantly better at the 3‐month follow‐up. No relation was found between changes in performance and the amount of displacement of the electrode array.Conclusions:After cochlear reimplantation with the same device, electrode‐array position can be accurately replicated and speech perception can be regained or even improved within weeks.
Follow‐up after gamma knife radiosurgery for vestibular schwannomas
Authors: Timmer Ferdinand C.A. , Hanssens Patrick E.J. , van Haren Anniek E. , van Overbeeke Jakobus J. , Mulder Jef J.S. , Cremers Cor W.R.J. , Graamans Kees 
Published: June 2011

AbstractObjectives/Hypothesis:A prospective long‐term follow‐up study was conducted to evaluate the results of gamma knife radiosurgery (GKRS) for vestibular schwannoma (VS) patients. Both axial and volumetric measurements are used to determine tumor size during follow‐up.Study Design:Individual prospective cohort study.Methods:A total of 110 VS patients were referred for radiosurgery between 2002 and 2007. All patients were treated with a Leksell 4C gamma knife. There were 12.5 to 13 Gy prescribed to the isodose covering 90% of the tumor volume. The resulting marginal dose was on average 11.0 Gy (range, 9.3–12.5 Gy). Tumor size and tumor volume were determined before and after gamma knife treatment at regular intervals. The minimal follow‐up period was 2 years.Results:There were 100 patients included in the study. Eight patients needed additional treatment after a mean follow‐up period of 38 months. One patient experienced a temporary facial nerve deficit. No growth pattern could be recognized for tumor growth after GKRS. Based on the measurements of the largest extrameatal diameter, the tumor size would have decreased or remained stable in 94%. Based on volumetric measurement, the tumor size was decreased or remained stable in 79%.Conclusions:High tumor control and low complication rates make GKRS a good therapy for VS. If tumor growth occurs after GKRS, a conservative management can be considered because continued tumor growth is uncertain. The extrameatal diameter on axial magnetic resonance imaging seems to be a reliable parameter of the size of a VS. Volumetry is the preferred method to assess the dimensions of a VS, although the consequences of strong volumetric increase, especially in small tumors, can be different depending on individual differences in tumor size.