bluuebunny/crossref_metadata_2025_split huggingface dataset:
VALIDITAS E-MODUL PhET INTERACTIVE SIMULATION DALAM MENINGKATKAN PEMAHAMAN KONSEP FISIKA TERINTEGRASI BIOLOGI BAGI MAHASISWA PENDIDIKAN BIOLOGI
Authors: Rizal Haryanti Putri , Genisa Marlina Ummas , Hasri  
Published: May 2024

Penelitian ini bertujuan untuk mengetahui validitas e-modul dengan PhET Interactive Simulation bagi mahasiswa Pendidikan Biologi dalam meningkatkan pemahaman konsep fisika terintegrasi biologi yakni gelombang dan bunyi pada makhluk hidup. Jenis penelitian ini adalah Research and Development (R&D) dengan model ADDIE, yakni analysis (analisis), design (perancangan), development (pengembangan), implementation (implementasi) dan evaluation (evaluasi). Pada penelitian ini hanya dilakukan sampai pada tahap pengembangan produk yang divalidasi oleh tiga ahli pendidikan dan pengajaran. Berdasarkan hasil validasi diperoleh persentase rata-rata sebesar 83% kategori sangat valid dengan validitas isi sebesar 85% dan validitas konstruk sebesar 81% masing-masing berada dalam kategori sangat valid, sehingga e-modul yang dikembangkan dinyatakan sangat valid dan layak untuk dilanjutkan ke tahap implementasi dan evaluasi. Rekomendasi praktis dari penelitian ini dapat digunakan dalam meningkatkan pemahaman konsep fisika terintegrasi biologi bagi mahasiswa Pendidikan Biologi dengan memanfaatkan teknologi simulasi virtual secara efektif.
The study aimed to determine the validity of e-module with PhET Interactive Simulation for biology education students in improving understanding of physics concepts integrated in biology, namely waves and sounds in living beings. The type of research was Research and Development (R&D) with ADDIE model, namely analysis, design, development, implementation, and evaluation. This research was only carried out up to the product development stage which was validated by three education and teaching experts. Based on the results of validation, an average percentage of 83% of the category is very valid with content validity of 85% and construct validity of 81% respectively in the highly valid category, so the developed e-module was declared very valid and worthy of being continued to the implementation and evaluation stages. The practical recommendation of this study can be used to improve the understanding of physics concepts integrated in biology for biology education students by effectively utilizing virtual simulation technology.
Pendampingan Pembuatan Lubang Resapan Biopori di Meruya Selatan
Authors: Citra Zel , Kinasih Reni Karno , Putri Mukhlisya Dewi Ratna , Girsang Hamonangan 
Published: May 2024

Minimnya ruang untuk resapan air di kota-kota besar seperti Jakarta, menimbulkan genangan-genangan, oleh sebab itu perlu antisipasi genangan air hujan, salah satunya adalah pembuatan Lubang Resapan Biopori (LRB). Metode alternatif Lubang Resapan Biopori (LRB) dipilih dikarenakan memiliki beberapa keunggulan diantaranya teknik pembuatannya mudah, operasinya sederhana, biaya yang dibutuhkan murah dan dapat dilakukan secara gotong royong. Selain berfungsi untuk meningkatkan luasan resapan air, keunggulan dari penerapan metode ini adalah pemanfaatan limbah organik yang dapat dikonversi menjadi pupuk kompos yang dapat dimanfaatkan oleh masyarakat. Kegiatan PkM ini dilaksanakan pada Rabu, 24 April 2024 di RPTRA Meruya Selatan yang dihadiri oleh 55 peserta termasuk Lurah Meruya Selatan. Berdasarkan hasil perhitungan skor kepentingan dan kinerja, peserta merasa sangat puas dengan materi yang disampaikan, karena sesuai dengan kebutuhan.
‘It was not Sinai’: Wayfaring with Nijinsky in and around St. Moritz. Some Reflections on Practising Dance History in the Field
Authors: Klimczyk Wojciech 
Published: May 2024

Among the most enigmatic passages in the so-called ‘diary’ of Vaslav Nijinsky are the four descriptions of the walks Nijinsky presumably took around St. Moritz where he moved in 1917. This article concerns research on these walks conducted by the author in St. Moritz, by means of wayfaring as conceptualized by Tim Ingold. It presents the current state of the sites mentioned by the dancer and hypothesizes about his routes and the dramatic effects of walks through the Upper Engandine landscape. It also ponders the impossibility of accessing the ‘original’ sites, and hence the walks as they happened, as well as the advantages of this impossibility for a more open dance history.
Effects of Trace Elements and Antimycotics to Manage Rice Blast Disease Caused by Pyricularia oryzae
Authors: Maira Fatima , Sahi Shahbaz T. , Ali Safdar , Khan Muhammad A. 
Published: May 2024

Rice blast disease caused by Pyricularia oryzae causes significant yield losses. Sixteen commercial cultivars of fine rice from Rice Research Institute, Kala Shah Kaku, Punjab, Pakistan were assessed for genomic resistance years as well as the efficacy of trace elements and antimycotics for two consecutive years (2021-22). Two varieties (Super Basmati and Basmati-370) were found highly susceptible, while KSK 133 was observed resistant, RC-8, CHECK, RC-7, PKBR21-8 and PK11876-1-2 were moderately resistant, KSK-282, RC-6, PKBR21-12,PKBR2-1 and PK11661-2-3 were moderately susceptible and the variety C1,C2 and C3 were susceptible against the disease. In management of the disease, combination of thiophanate methyl, tebuconazole + trifloxystrobin, and fosetyle-aluminium was more effective with minimum disease severity of 11.89% and 11.69%. In a moderatley susceptible vareity the application of thiophanate methyl and fosetyle-aluminium also effectively suppressed the disease severity 17.83% and 16.90% respectively. Tebuconazole + trifloxystrobin combination was less effective against the disease (36.67% and 36.51%). The combination of copper, zinc, and boron suppressed the disease to the maximum with disease severity of 9.681% and 9.62%. Overall, respective combinations of both fungicides and trace elements were found to be the most effective in controlling rice blast disease
Detection of calcitonin gene-related peptide based on increased antigen-driven interaction with antibody variable regions
Authors: Cheng Yueqing , Gao Yujie , Zhang Shengshuo , Zou Yujie , Zhao Guangwei , Zheng Liyuan , Hou Binghui , Li Mei , Dong Jinhua 
Published: May 2024

Introduction: Calcitonin gene-related peptide (CGRP) is involved in trigeminal neuralgia and migraine, and measuring the CGRP concentration in the serum is crucial for the early prediction of these conditions. Current methods for CGRP detection are primarily radioimmunoassay, which needs radioactive substances and enzyme-linked immunosorbent assays (ELISAs) which need long detection time and some have a narrow detection range.Methods: The genes of anti-CGRP antibody variable regions were cloned into pDong1 vector to obtain pDong1/Fab-CGRP, with which phage-Fab was prepared, and the concentration of CGRP was detected by competitive ELISA. The pDong1/Fab-CGRP was modified to obtain pDong1/OS-CGRP, with which the co-expression solution containing phage-displayed heavy chain variable fragments (phage-VH) and light chain was obtained. CGRP was detected by OS-ELISA based on phage-VH, antibody light chain, and anti-light chain antibody. The VL gene was cloned into the pMAL vector to obtain pMAL-VL (CGRP), with which maltose binding protein fused with VL (MBP-VL) was prepared. CGRP was detected by OS-ELISA employing MBP-VL and phage-VH.Results: OS-ELISAs that measure the CGRP concentration by quantifying the interaction between variable regions were investigated. OS-ELISA using phage-VH and secreted light chains in the same culture system exhibited a limit of detection (LOD) of 0.05 nM, offering higher sensitivity than competitive assay with an LOD of 0.75 nM, whereas using phage-VH and separately prepared MBP-VL exhibited an LOD of 0.15 nM and a broader detection range of 0.15–500 nM than competitive ELISA, whose detection range was 0.75–10 nM.Discussion: The combination of the two OS assays achieved high sensitivity and a broad detection range for CGRP, which may have significance in clinical applications.
Prenylflavonoids isolated from Epimedii Herba show inhibition activity against advanced glycation end-products
Authors: Nakashima Keisuke , Miyashita Hiroyuki , Yoshimitsu Hitoshi , Fujiwara Yukio , Nagai Ryoji , Ikeda Tsuyoshi 
Published: May 2024

Introduction: As inhibitors of advanced glycation end products (AGEs), such as pyridoxamine, significantly inhibit the development of retinopathy and neuropathy in rats with streptozotocin-induced diabetes, treatment with AGE inhibitors is believed to be a potential strategy for the prevention of aging, age-related diseases, and lifestyle-related diseases, including diabetic complications. In the present study, the MeOH extract of Epimedii Herba (EH; aerial parts of Epimedium spp.) was found to inhibit the formation of Nε-(carboxymethyl)lysine (CML) and Nω-(carboxymethyl) arginine (CMA) during the incubation of collagen-derived gelatin with ribose.Materials and methods: EH was purchased from Uchida Wakan-yaku Co., and a MeOH extract was prepared. Several steps of column chromatography purified the extract. Each fraction was tested for inhibitory activity by ELISA using monoclonal antibodies for CML and CMA.Results: After activity-guided fractionation and purification by column chromatography, three new prenylflavonoids [named Koreanoside L (1), Koreanoside E1 (2), and Koreanoside E2 (3)] and 40 known compounds (4–43) were isolated from EH, and their inhibitory effects against CML and CMA formation were tested. Among these, epimedokoreanin B (8), epimedonin E (21), epicornunin B (22), and epicornunin F (24) inhibited the formation of both CML and CMA, with epimedokoreanin B (8) having the most potent inhibitory effect among the isolated compounds. To obtain the structure–activity relationships of 8, the phenolic hydroxy groups of 8 were methylated by trimethylsilyl-diazomethane to afford the partially and completely methylated compounds of 8. Prenyl derivatives of propolis (artepillin C, baccharin, and drupanin) were used in the assay.Discussion: As only 8 showed significant activity among these compounds, the catechol group of the B ring and the two prenyl groups attached to the flavanone skeleton were essential for activity. These data suggest that 8 could prevent the clinical complications of diabetes and age-related diseases by inhibiting AGEs.
Optical coherence tomography-guided vs. intravascular ultrasound-guided percutaneous coronary intervention: a systematic review and meta-analysis of randomized controlled trials
Authors: Vats Vaibhav , Elahi Aarij , Hidri Sinda , Abdelkader Rem Ehab , Munaf Farhan , Prince Jennifer Mercika , Asif Muhammad Ahsan , Cheema Huzaifa Ahmad , Ahmad Adeel , Rehman Wajeeh Ur , Nashwan Abdulqadir J. , Ahmed Raheel , Lakhter Vladimir , Virk Hafeez Ul Hassan , Vincent Royce P. 
Published: May 2024

BackgroundOptical coherence tomography (OCT) and intravascular ultrasound (IVUS) are superior to coronary angiography for guiding percutaneous coronary intervention (PCI). However, whether one technique is superior to the other is inconclusive.MethodsWe searched PubMed, Embase, the Cochrane Library, and ClinicalTrials.gov from inception to November 2023 for randomized controlled trials (RCTs) comparing OCT and IVUS in patients undergoing PCI. RevMan 5.4 was used to pool outcomes with risk ratio (RR) as the effect measure.ResultsSix RCTs (4,402 patients) were included in this meta-analysis. There was no significant difference between the OCT- and IVUS-guided PCI groups in the risk of major adverse cardiovascular events (RR 0.87, 95% CI: 0.65, 1.16; I2 = 0%) and cardiac mortality (RR 0.73, 95% CI: 0.24, 2.21; I2 = 0%). The results were consistent across the subgroups of the presence or absence of left main disease (Pinteraction &gt;0.1). There were no significant differences between OCT and IVUS in the risk of target lesion revascularization (RR 0.78, 95% CI: 0.47, 1.30; I2 = 0%), target vessel revascularization (RR 1.06, 95% CI: 0.69, 1.62; I2 = 0%), target-vessel myocardial infarction (RR 0.79, 95% CI: 0.40, 1.53; I2 = 0%), stent thrombosis (RR 0.59, 95% CI: 0.12, 2.97; I2 = 0%), and all-cause mortality (RR 1.01, 95% CI: 0.53, 1.90; I2 = 0%).ConclusionsOur meta-analysis demonstrated similar clinical outcomes in OCT- and IVUS-guided PCI. New large-scale multicenter RCTs with long-term follow-up are required to confirm or refute our findings and provide more reliable results.Systematic Review RegistrationPROSPERO, identifier, CRD42023486933
Case Report: Lacosamide unmasking SCN5A-associated Brugada syndrome in a young female with epilepsy
Authors: Shen Ying-Chi , Wu Jen-Chueh , Lin Ting-Tse , Chang Kai-Chung , Su Jen-Jen , Juang Jyh-Ming Jimmy 
Published: May 2024

BackgroundLacosamide is frequently used as a mono- or adjunctive therapy for the treatment of adults with epilepsy. Although lacosamide is known to act on both neuronal and cardiac sodium channels, potentially leading to cardiac arrhythmias, including Brugada syndrome (BrS), its adverse effects in individuals with genetic susceptibility are less understood.CaseWe report a 33-year-old female with underlying epilepsy who presented to the emergency department with a four-day history of seizure clusters, and was initially treated with lacosamide therapy. During the intravenous lacosamide infusion, the patient developed sudden cardiac arrest caused by ventricular arrhythmias necessitating resuscitation. Of note, the patient had a family history of sudden cardiac death. Workup including routine laboratory results, 12-lead electrocardiogram (ECG), echocardiogram, and coronary angiogram was non-specific. However, a characteristic type 1 Brugada ECG pattern was identified by ajmaline provocation testing; thus, confirming the diagnosis of BrS. Subsequently, the genotypic diagnosis was confirmed by Sanger sequencing, which revealed a heterozygous mutation (c.2893C&gt;T, p.Arg965Cys) in the SCN5A gene. Eventually, the patient underwent implantable cardioverter-defibrillator implantation and was discharged with full neurological recovery.ConclusionThis case highlights a rare but lethal adverse event associated with lacosamide treatment in patients with genetic susceptibility. Further research is warranted to investigate the interactions between lacosamide and SCN5A variants.
Depletion of follicular B cell-derived antibody secreting cells does not attenuate angiotensin II-induced hypertension or vascular compliance
Authors: Figueiredo Galvao Hericka Bruna , Lieu Maggie , Moodley Seyuri , Diep Henry , Jelinic Maria , Bobik Alexander , Sobey Christopher G. , Drummond Grant R. , Vinh Antony 
Published: May 2024

IntroductionMarginal zone and follicular B cells are known to contribute to the development of angiotensin II-induced hypertension in mice, but the effector function(s) mediating this effect (e.g., antigen presentation, antibody secretion and/or cytokine production) are unknown. B cell differentiation into antibody secreting cells (ASCs) requires the transcription factor Blimp-1. Here, we studied mice with a Blimp-1 deficiency in follicular B cells to evaluate whether antibody secretion underlies the pro-hypertensive action of B cells.Methods10- to 14-week-old male follicular B cell Blimp-1 knockout (FoB-Blimp-1-KO) and floxed control mice were subcutaneously infused with angiotensin II (0.7 mg/kg/d) or vehicle (0.1% acetic acid in saline) for 28 days. BP was measured by tail-cuff plethysmography or radiotelemetry. Pulse wave velocity was measured by ultrasound. Aortic collagen was quantified by Masson's trichrome staining. Cell types and serum antibodies were quantified by flow cytometry and a bead-based multiplex assay, respectively.ResultsIn control mice, angiotensin II modestly increased serum IgG3 levels and markedly increased BP, cardiac hypertrophy, aortic stiffening and fibrosis. FoB-Blimp-1-KO mice exhibited impaired IgG1, IgG2a and IgG3 production despite having comparable numbers of B cells and ASCs to control mice. Nevertheless, FoB-Blimp-1-KO mice still developed hypertension, cardiac hypertrophy, aortic stiffening and fibrosis following angiotensin II infusion.ConclusionsInhibition of follicular B cell differentiation into ASCs did not protect against angiotensin II-induced hypertension or vascular compliance. Follicular B cell functions independent of their differentiation into ASCs and ability to produce high-affinity antibodies, or other B cell subtypes, are likely to be involved in angiotensin II-induced hypertension.
Validation of a Spanish version of the Foreign Language Classroom Anxiety Scale in Peruvian secondary education students
Authors: Quispe-Sanca Ruth V. , Arteta-Sandoval Gissel , Quispe-Sanca David , Morales-García Wilter C. 
Published: May 2024

BackgroundAnxiety in learning foreign languages is a global phenomenon that impacts students’ academic performance, with English being a critical language in secondary education. The Foreign Language Classroom Anxiety Scale (FLCAS) has been widely used to assess this anxiety. However, the need to validate and adapt the scale for specific cultural contexts remains a priority in educational research.ObjectiveTo assess the validity and reliability of a Spanish version of the FLCAS among a sample of Peruvian secondary education students, in order to better understand the structure of language learning anxiety in this context.MethodsA total of 818 students from four public educational institutions in southern Peru participated. An instrumental design was used, which included descriptive analysis, exploratory factor analysis (EFA), confirmatory factor analysis (CFA), and assessments of convergent, divergent validity, and measurement invariance by gender.ResultsThe EFA recommended a four-factor model that explained 32% of the total variance. Subsequent CFA adjusted this model to 24 items with optimal fit indices (CFI = 0.969; RMSEA = 0.045). The reliability of the factors was acceptable (α &gt; 0.70 for all factors). Convergent and divergent validity was confirmed through significant correlations with related constructs (Fear of Negative Evaluation and Enjoyment of Foreign Language). Gender invariance analysis indicated that the scale is equally applicable to men and women.ConclusionThe Spanish version of the FLCAS is a valid and reliable tool for assessing foreign language classroom anxiety among Peruvian secondary education students. This study contributes to the field of language teaching by providing evidence of the cultural applicability of the FLCAS in the Peruvian context, thus facilitating more effective pedagogical interventions to address language learning anxiety.
Gut-bone axis research: unveiling the impact of gut microbiota on postmenopausal osteoporosis and osteoclasts through Mendelian randomization
Authors: Xiao Hefang , Wang Yaobin , Chen Yi , Chen Rongjin , Yang Chenhui , Geng Bin , Xia Yayi 
Published: May 2024

BackgroundPostmenopausal osteoporosis is a prevalent disease that affects the bone health of middle-aged and elderly women. The link between gut microbiota and bone health, known as the gut-bone axis, has garnered widespread attention.MethodsWe employed a two-sample Mendelian randomization approach to assess the associations between gut microbiota with osteoclasts and postmenopausal osteoporosis, respectively. Single nucleotide polymorphisms associated with the composition of gut microbiota were used as instrumental variables. By analyzing large-scale multi-ethnic GWAS data from the international MiBioGen consortium, and combining data from the eQTLGen consortium and the GEFOS consortium, we identified microbiota related to osteoclasts and postmenopausal osteoporosis. Key genes were further identified through MAGMA analysis, and validation was performed using single-cell data GSE147287.ResultsThe outcomes of this study have uncovered significant associations within the gut microbiome community, particularly with the Burkholderiales order, which correlates with both an increase in osteoclasts and a reduced risk of postmenopausal osteoporosis. with an odds ratio (OR) of 0.400, and a P-value of 0.011. Further analysis using single-cell data allowed us to identify two key genes, FMNL2 and SRBD1, that are closely linked to both osteoclasts and osteoporosis.ConclusionThis study utilizing Mendelian randomization and single-cell data analysis, provides new evidence of a causal relationship between gut microbiota and osteoclasts, as well as postmenopausal osteoporosis. It was discovered that the specific microbial group, the Burkholderiales order, significantly impacts both osteoporosis and osteoclasts. Additionally, key genes FMNL2 and SRBD1 were identified, offering new therapeutic strategies for the treatment of postmenopausal osteoporosis.
Transcobalamin 2 orchestrates monocyte proliferation and TLR4-driven inflammation in systemic lupus erythematosus via folate one-carbon metabolism
Authors: Liu Baoyi , Li Ang , Liu Yi , Zhou Xinzhu , Xu Jingkai , Zuo Xianbo , Xue Ke , Cui Yong 
Published: May 2024

BackgroundSLE is a complex autoimmune disease with deleterious effects on various organs. Accumulating evidence has shown abnormal vitamin B12 and one-carbon flux contribute to immune dysfunction. Transcobalamin II (TCN2) belongs to the vitamin B12-binding protein family responsible for the cellular uptake of vitamin B12. The role of TCN2 in SLE is still unclear.MethodsWe collected clinical information and blood from 51 patients with SLE and 28 healthy controls. RNA sequencing analysis, qPCR, and western blot confirmed the alteration of TCN2 in disease monocytes. The correlation between TCN2 expression and clinical features and serological abnormalities was analyzed. TCN2 heterozygous knockout THP1 cells were used to explore the effects of TCN2 dysfunction on monocytes. CCK-8 assay and EdU staining were used to detect cell proliferation. ELISA was conducted to assess vitamin B12, glutathione, and cytokines changes. UHPLC-MRM-MS/MS was used to detect changes in the intermediates of the one-carbon cycle. Flow cytometry is used to detect cell cycle, ROS, mitoROS, and CD14 changes.ResultsElevated TCN2 in monocytes was correlated positively with disease progression and specific tissue injuries. Using CD14+ monocytes and TCN2 genetically modified THP1 cell lines, we found that the TCN2 was induced by LPS in serum from SLE patients. TCN2 heterozygous knockout inhibited cellular vitamin B12 uptake and one-carbon metabolism, leading to cell proliferation arrest and decreased Toll-like receptor 4 (TLR4)-mediated CCL2 release. Methionine cycle metabolites, s-adenosylmethionine and homocysteine, rescued these effects, whereas folate treatment proved to be ineffective. Folate deficiency also failed to replicate the impact of TCN2 downregulation on THP1 inflammatory response.ConclusionOur study elucidated the unique involvement of TCN2-driven one-carbon flux on SLE-associated monocyte behavior. Increased TCN2 may promote disease progression and tissue damage by enhancing one-carbon flux, fostering monocyte proliferation, and exacerbating TLR4 mediated inflammatory responses. The inhibition of TCN2 may be a promising therapeutic approach to ameliorate SLE.