bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fcγ receptor‐mediated antigen uptake by lung DC contributes to allergic airway hyper‐responsiveness and inflammation
Authors: Hartwig Christina , Mazzega Miriam , Constabel Hannelore , Krishnaswamy Jayendra K. , Engelbert Gessner J. , Braun Armin , Tschernig Thomas , Behrens Georg M. N. 
Published: February 2010

AbstractDuring asthma, lung DC capture and process antigens to initiate and maintain allergic Th2 cell responses to inhaled allergens. The aim of the study was to investigate whether allergen‐specific IgG, generated during sensitization, can potentiate the acute airway inflammation through Fcγ receptor (FcγR)‐mediated antigen uptake and enhance antigen presentation resulting in augmented T‐cell proliferation. We examined the impact of antigen presentation and T‐cell stimulation on allergic airway hyperresponsiveness and inflammation using transgenic and gene‐deficient mice. Both airway inflammation and eosinophilia in bronchoalveolar lavage fluid were markedly reduced in sensitized and challenged FcγR‐deficient mice. Lung DC of WT, but not FcγR‐deficient mice, induced increased antigen‐specific CD4+ T‐cell proliferation when pulsed with anti‐OVA IgG immune complexes. Intranasal application of anti‐OVA IgG immune complexes resulted in enhanced airway inflammation, eosinophilia and Th2 cytokine release, mediated through enhanced antigen‐specific T‐cell proliferation in vivo. Finally, antigen‐specific IgG in the serum of sensitized mice led to a significant increase of antigen‐specific CD4+ T‐cell proliferation induced by WT, but not FcγR‐deficient, lung DC. We conclude that FcγR‐mediated enhanced antigen presentation and T‐cell stimulation by lung DC has a significant impact on inflammatory responses following allergen challenge in asthma.
Blockade of GITR–GITRL interaction maintains Treg function to prolong allograft survival
Authors: Kim James I. , Sonawane Samsher B. , Lee Major K. , Lee Seoung‐Hoon , Duff Patrick E. , Moore Daniel J. , O'Connor Matthew R. , Lian Moh‐Moh , Deng Shaoping , Choi Yongwon , Yeh Heidi , Caton Andrew J. , Markmann James F. 
Published: February 2010

AbstractInvolvement of Treg in transplant tolerance has been demonstrated in multiple models. During the active process of graft rejection, these regulatory cells are themselves regulated and inactivated, a process termed counter‐regulation. We hypothesize that ligation of the costimulatory molecule glucocorticoid‐induced TNF receptor‐related protein (GITR) on Treg inhibits their ability to promote graft survival, and by blocking GITR ligation graft survival can be prolonged. To this aim, we have designed a soluble GITR fusion protein (GITR‐Fc), which binds GITR ligand and inhibits activation of GITR. Here, we show that GITR‐Fc prolonged mouse skin graft survival, and this prolongation is dependent on Treg. In a full MHC‐mismatched skin graft setting, GITR‐Fc significantly improved graft survival when used in combination with MR1, anti‐CD40L, while GITR‐Fc alone did not demonstrate graft prolongation. These results demonstrate that disruption of binding of GITR with GITR ligand may be an important strategy in prolonging allograft survival.
Synthesis, Structures, and Properties of New Chalcogenide‐Capped Octahedral Hexatechnetium(III) Complexes [Tc6S8X6]4– (X = Br, I), [Tc6Se8I2], and [Tc6Te15]
Authors: Yoshimura Takashi , Ikai Takuya , Tooyama Yuji , Takayama Tsutomu , Sekine Tsutomu , Kino Yasushi , Kirishima Akira , Sato Nobuaki , Mitsugashira Toshiaki , Takahashi Naruto , Shinohara Atsushi 
Published: February 2010

AbstractChalcogenide‐capped octahedral hexatechnetium(III) complexes, Cs4[Tc6S8Br6]·CsBr (Cs4[1]·CsBr) and (PPh4)4[Tc6S8Br6] {(PPh4)4[1]}, (PPh4)4[Tc6S8I6] {(PPh4)4[2]}, [Tc6Se8I2] ([3]), and [Tc6Te15] ([4]) were synthesized. Single‐crystal X‐ray structural studies were carried out for Cs4[1]·CsBr, [3], and [4]. Sulfide‐capped octahedral hexatechnetium(III) clusters [1]4– and [2]4– are discrete complex anions, whereas the selenide‐ and telluride‐capped hexatechnetium(III) complexes adopt extended polymeric structures. A cyclic voltammogram of [1]4– in CH3CN showed two one‐electron redox waves: Tc6(24e/23e) and Tc6(23e/22e). The potential of the Tc6(24e/23e) process is more positive than that of the Re6(24e/23e) process for the hexarhenium analog [Re6S8X6]4– (X = Br, I), whereas that of the Tc6(23e/22e) process shifts to negative values compared to the potential of the Re6(23e/22e) process. Thus, the Tc6(23e) mixed‐valence state is thermodynamically less stable than Re6(23e).
First Asymmetric Synthesis of Boehmeriasin A
Authors: Dumoulin David , Lebrun Stéphane , Couture Axel , Deniau Eric , Grandclaudon Pierre 
Published: February 2010

AbstractThe first asymmetric synthesis of phenanthroquinolizidinealkaloid (R)‐boehmeriasin A is described. Two alternative synthetic pathways to the key intermediate (RS,R)‐4 were achieved through a combination of highly diastereoselective 1,2‐nucleophilic addition on (–)‐(S)‐1‐amino‐2‐(methoxymethyl)pyrrolidine hydrazones with a ring‐closing metathesis to ensure the construction of the piperidine template. A subsequent acylation/oxidation/aldol condensation/radical cyclization sequence completed the assembly of the title (R)‐configured natural product.
Reductive Amination/Cyclization of ω‐Trifluoromethyl Keto Esters to Trifluoromethylated δ‐Amino Alcohols and Lactams
Authors: Wan Wen , Hou Jie , Jiang Haizhen , Yuan Zongqian , Ma Goubin , Zhao Gang , Hao Jian 
Published: February 2010

AbstractA reductive amination/cyclization approach towards biologically interesting trifluoromethylated four‐ to seven‐membered ring lactams from simply prepared ω‐trifluoromethyl keto esters in good to excellent yields has been developed. In addition, trifluoromethylated δ‐amino alcohols were also obtained directly from an unexpected reduction of the corresponding γ‐imino esters in the presence of an excess amount of NaBH4.
PANDAS and anorexia nervosa—a spotters' guide: Suggestions for medical assessment
Authors: Vincenzi Brenda , O'Toole Julie , Lask Bryan 
Published: February 2010

AbstractObjectivePediatric Autoimmune Neuropsychiatric Disorders Associated with Streptococcal infection (PANDAS) should be considered in sudden onset, prepubertal Anorexia Nervosa (AN), arising shortly after an apparent streptococcal infection. However, the absence of a specific biological marker of PANDAS renders the diagnosis difficult. This paper critically reviews available tests for PANDAS and recommends a standardized approach to its investigation.MethodMedline database review between 1990 and 2008.ResultsExisting tests may be categorized as: (i) Non‐specific markers of inflammation or immune response (Erythrocyte sedimentation rate, ESR; C‐reactive protein, CRP; Neopterin), (ii) specific markers of streptococcal infection (throat swab and anti‐streptococcal antibodies, Anti‐streptolysin, ASO; Antideoxyribonuclease B, antiDNaseB), (iii) non‐specific markers of auto‐immune reaction (Antineuronal antibodies, AnAb; D8/17). No one test reliably identifies PANDAS. The lack of specificity and methodological problems may lead to errors of diagnosis.DiscussionWhen PANDAS–Anorexia Nervosa (PANDAS–AN) is suspected clinically we recommend conducting all the above investigations. The more positive results there are the more likely is the diagnosis, but particular weighting should be given to AnAb and D8/17. Copyright © 2010 John Wiley & Sons, Ltd and Eating Disorders Association.
Feelings of insecurity: Review of attachment and eating disorders
Authors: Zachrisson Henrik Daae , Skårderud Finn 
Published: February 2010

AbstractObjectiveAttachment theory has received increasing attention from clinicians and researchers in the field of eating disorders. This paper is an updated review on theoretical approaches in the field, and of studies employing the Adult Attachment Interview.MethodWe searched the major databases such as PsycInfo and Science Direct for empirical and theoretical studies on attachment and eating disorders, and these are reviewed.ResultsTheoretical approaches on attachment and eating disorders highlight either retrospective, general risk or attachment theoretical statements. There is greater prevalence of insecure attachment in the eating disordered population than in non‐clinical samples. However, there is no sufficient evidence to conclude about specific mechanisms for this connection.DiscussionThe theoretical approaches vary in their theoretical grounding, specificity and empirical support. AAI‐based research on attachment and eating disorders is still at an early stage. More advanced studies are required for the field to move forward. Copyright © 2010 John Wiley & Sons, Ltd. and Eating Disorders Association.
Bacterial cytotoxicity and induction of apoptosis in promyelocytic (Line HL-60) cells by novel organotellurium compounds
Authors: Sailer Brian L , Prow Tarl , Dickerson Sarah , Watson John , Liles Nathan , Patel Shiv J , Van Fleet-Stalder Verena , Chasteen Thomas G 
Published: February 2010

Abstract
The increasing importance of tellurium in organic synthesis, in industrial applications, and as a possible component in pesticides means that its introduction into the environment will probably increase in the future. A knowledge of the relative toxicity and the mode of toxic action of tellurium-containing chemicals is therefore important. The work presented here, using growth-rate inhibition effects on Pseudomonas fluorescens of two model compounds, telluranthrene and tetrachlorodiphenyl ditelluride, suggests that these compounds are at least as toxic as the relatively well-known selenium oxyanions, selenate and selenite. Experiments with human promyelocytic (line HL-60) cells indicate that both the above organotellurium compounds induce a form of cell death called apoptosis, or programmed cell death. The induction of apoptosis occurred in both a time- and dose-dependent manner, as assayed by three different analytical methods: fluorescence microscopy, flow cytometry, and gel electrophoresis. The results indicate that organotellurium compounds may find applicability in the fields of chemoprevention and cancer therapy, similar to organoselenium compounds.
Connective tissue growth factor associated with oncogenic activities and drug resistance in glioblastoma multiforme
Authors: Yin Dong , Chen Weikai , O'Kelly James , Lu Daning , Ham Michelle , Doan Ngan B. , Xie Dong , Wang Charles , Vadgama Jay , Said Jonathan W. , Black Keith L. , Koeffler H. Phillip 
Published: February 2010

AbstractConnective tissue growth factor (CTGF or CCN2) is a secreted protein that belongs to the CCN [cysteine‐rich CYR61/CTGF/nephroblastoma‐overexpressed gene] family. These proteins have been implicated in various biological processes, including stimulation of cell proliferation, migration, angiogenesis and tumorigenesis. In a previous study, we found that CTGF mRNA was elevated in primary gliomas, and a significant correlation existed between CTGF mRNA levels versus tumor grade, histology and patient survival. In this study, the role of CTGF in glioma tumorigenesis was explored. Forced expression of CTGF in glioblastoma multiforme (GBM) cells accelerated their growth in liquid culture and soft agar, stimulated cells migration in Boyden chamber assays and significantly increased their ability to form large, vascularized tumors in nude mice. CTGF induced the expression of the antiapoptotic proteins, Bcl‐xl, Survivin and Flip. Overexpression of CTGF caused the U343 GBM cells to survive for longer than 40 days in serum‐free medium and resist antitumor drugs including tumor necrosis factor (TNF), TNF‐related apoptosis‐inducing ligand, VELCADE (bortezomib, proteasome inhibitor) and temozolomide. Our data suggest that CTGF plays an important role in glioma progression, by supporting tumor cells survival and drug resistance.
miR‐181b modulates multidrug resistance by targeting BCL2 in human cancer cell lines
Authors: Zhu Wei , Shan Xia , Wang Tongshan , Shu Yongqian , Liu Ping 
Published: February 2010

AbstractMicroRNAs (miRNAs) are short noncoding RNA molecules, which posttranscriptionally regulate genes expression and play crucial roles in diverse biological processes, such as development, differentiation, apoptosis and proliferation. Here, we investigated the possible role of miRNAs in the development of multidrug resistance (MDR) in human gastric and lung cancer cell lines. We found that miR‐181b was downregulated in both multidrug‐resistant human gastric cancer cell line SGC7901/vincristine (VCR) and multidrug‐resistant human lung cancer cell line A549/cisplatin (CDDP), and the downregulation of miR‐181b in SGC7901/VCR and A549/CDDP cells was concurrent with the upregulation of BCL2 protein, compared with the parental SGC7901 and A549 cell lines, respectively. In vitro drug sensitivity assay demonstrated that overexpression of miR‐181b sensitized SGC7901/VCR and A549/CDDP cells to anticancer drugs, respectively. The luciferase activity of a BCL2 3′‐untranslated region‐based reporter construct in SGC7901/VCR and A549/CDDP cells suggests that a new target site in the 3′UTR of BCL2 of the mature miR‐181s (miR‐181a, miR‐181b, miR‐181c and miR‐181d) was found. Enforced miR‐181b expression reduced BCL2 protein level and sensitized SGC7901/VCR and A549/CDDP cells to VCR‐induced and CDDP‐induced apoptosis, respectively. Taken together, our findings suggest that miR‐181b could play a role in the development of MDR in both gastric and lung cancer cell lines, at least in part, by modulation of apoptosis via targeting BCL2.
PALM imaging and cluster analysis of protein heterogeneity at the cell surface
Authors: Owen Dylan M. , Rentero Carles , Rossy Jérémie , Magenau Astrid , Williamson David , Rodriguez Macarena , Gaus Katharina 
Published: February 2010

AbstractThe authors employed photoactivatable localization microscopy (PALM) and direct stochastic optical reconstruction microscopy (dSTORM) imaging and image analysis based on Ripley's K ‐function to quantify the distribution and heterogeneity of proteins at the cell plasma membrane. The membrane targeting sequence of the N‐terminal region of the T cell receptor‐pathway kinase Lck fused to the photo‐convertible fluorescent protein tdEos (LckN10‐tdEos), clusters into sub‐100 nm regions which cover ∼7% of the cell surface. 2‐channel PALM imaging of LckN10‐tdEos and the N‐terminus of the kinase Src (SrcN15‐PS‐CFP2) are demonstrated. Finally, T cell microclusters at the immune synapse are imaged at super‐resolution using dSTORM, showing that conventional TIRF images contain unresolved, small clusters. These methods are generally applicable to other cell and fluorophore systems to quantify 2‐D molecular clustering at nanometer scales. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Solid freeform fabrication and characterization of porous calcium polyphosphate structures for tissue engineering purposes
Authors: Shanjani Yaser , De Croos J. N. Amritha , Pilliar Robert M. , Kandel Rita A. , Toyserkani Ehsan 
Published: February 2010

AbstractSolid freeform fabrication (SFF) enables the fabrication of anatomically shaped porous components required for formation of tissue engineered implants. This article reports on the characterization of a three‐dimensional‐printing method, as a powder‐based SFF technique, to create reproducible porous structures composed of calcium polyphosphate (CPP). CPP powder of 75–150 μm was mixed with 10 wt % polyvinyl alcohol (PVA) polymeric binder, and used in the SFF machine with appropriate settings for powder mesh size. The PVA binder was eliminated during the annealing procedure used to sinter the CPP particles. The porous SFF fabricated components were characterized using scanning electron microscopy, micro‐CT scanning, X‐ray diffraction, and mercury intrusion porosimetry. In addition, mechanical testing was conducted to determine the compressive strength of the CPP cylinders. The 35 vol % porous structures displayed compressive strength on average of 33.86 MPa, a value 57% higher than CPP of equivalent volume percent porosity made through conventional gravity sintering. Dimensional deviation and shrinkage analysis was conducted to identify anisotropic factors required for dimensional compensation during SFF sample formation and subsequent sintering. Cell culture studies showed that the substrate supported cartilage formation in vitro, which was integrated with the top surface of the porous CPP similar to that observed when chondrocytes were grown on CPP formed by conventional gravity sintering methods as determined histologically and biochemically. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Cells of extraembryonic mesodermal origin confer human dystrophin in the Mdx model of duchenne muscular dystrophy
Authors: Kawamichi Yayoi , Cui Chang‐Hao , Toyoda Masashi , Makino Hatsune , Horie Akane , Takahashi Yoriko , Matsumoto Kenji , Saito Hirohisa , Ohta Hiroaki , Saito Kayoko , Umezawa Akihiro 
Published: February 2010

AbstractDuchenne muscular dystrophy is an X‐linked recessive genetic disease characterized by severe skeletal muscular degeneration. The placenta is considered to be a promising candidate cell source for cellular therapeutics because it contains a large number of cells and heterogenous cell populations with myogenic potentials. We analyzed the myogenic potential of cells obtained from six parts of the placenta, that is, umbilical cord, amniotic epithelium, amniotic mesoderm, chorionic plate, villous chorion, and decidua basalis. In vitro cells derived from amniotic mesoderm, chorionic plate, and villous chorion efficiently transdifferentiate into myotubes. In addition, in vivo implantation of placenta‐derived cells into dystrophic muscles of immunodeficient mdx mice restored sarcolemmal expression of human dystrophin. Differential contribution to myogenesis in this study may be attributed to placental portion‐dependent default cell state. Molecular taxonomic characterization of placenta‐derived maternal and fetal cells in vitro will help determine the feasibility of cell‐based therapy. J. Cell. Physiol. 223:695–702, 2010. © 2010 Wiley‐Liss, Inc.
Carboxy terminus of secreted phosphoprotein‐24 kDa (spp24) is essential for full inhibition of BMP‐2 activity
Authors: Brochmann Elsa J. , Simon Robert J. , Jawien Janusz , Behnam Keyvan , Sintuu Chananit , Wang Jeffrey C. , Murray Samuel S. 
Published: February 2010

AbstractSecreted phosphoprotein‐24 kDa (spp24) is a bone morphogenetic protein (BMP)‐binding protein isolated from bone. It exists in a number of size forms and is hypothesized to function as a BMP latency protein and/or a “slow release” mechanism for BMPs involved in bone turnover and repair. We have examined the hypothesis that proteolytic modification of the C‐terminus of spp24 affects its BMP‐2–binding properties and bioactivity in the BMP‐2–stimulated ectopic bone forming bioassay. Three different size forms of recombinant spp24 that correspond to predicted 18.1 kDa, 16.0 kDa, and 14.5 kDa proteolytic products were compared to full‐length (fl) spp24. One of these forms (spp18.1) we hypothesize to be the protein which Urist initially, but apparently inaccurately, called “BMP.” Only full‐length spp24 completely inhibited BMP‐2–induced bone formation. The 18.1 kDa truncated isoform of spp24 which we hypothesize to be Urist's protein did not. The inhibitory capacity of the proteins was correlated with their kinetic constants, assessed by surface plasmon resonance. At the highest, inhibitory, dose of spp24 and its derivatives, kd (“stability”) best predicted the extent of ectopic bone formation whereas at the lowest dose, which was not inhibitory, ka (“recognition”) best predicted the extent of ectopic bone formation. We conclude that proteolytic processing of spp24 affects the interaction of this protein with BMP‐2 and this affects the function of the protein. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1200–1207, 2010
FT‐Raman, FT‐IR spectra and DFT calculations on monomeric and dimeric structures of 5‐fluoro‐ and 5‐chloro‐salicylic acid
Authors: Karabacak Mehmet , Kose Etem , Kurt Mustafa 
Published: February 2010

AbstractThe experimental and theoretical study on the structures and vibrations of 5‐fluoro‐salicylic acid and 5‐chloro‐salicylic acid (5‐FSA and 5‐ClSA, C7H5FO3 and C7H5ClO3) is presented. The Fourier transform infrared spectra (4000–400 cm−1) and the Fourier transform Raman spectra (4000–50 cm−1) of the title molecules in the solid phase were recorded. The molecular structures, vibrational wavenumbers, infrared intensities, Raman intensities and Raman scattering activities were calculated for a pair of molecules linked by the intermolecular OH···O hydrogen bond. The geometrical parameters and energies of 5‐FSA and 5ClSA were obtained for all eight conformers/isomers from density functional theory (DFT) (B3LYP) with 6‐311++G(d,p) basis set calculations. The computational results identified the most stable conformer of 5‐FSA and 5‐ClSA as the C1 form. The complete assignments were performed on the basis of the total energy distribution (TED) of the vibrational modes, calculated with scaled quantum mechanics (SQM) method. The spectroscopic and theoretical results were compared with the corresponding properties for 5‐FSA and 5‐ClSA monomers and dimer of C1 conformer. The optimized bond lengths, bond angles and calculated wavenumbers showed the best agreement with the experimental results. Copyright © 2010 John Wiley & Sons, Ltd.
Development of low‐fat mayonnaise containing polysaccharide gums as functional ingredients
Authors: Su Hou‐Pin , Lien Chuang‐Ping , Lee Tan‐Ang , Ho Jou‐Hsuan 
Published: February 2010

AbstractBACKGROUND: The objective of this study was to develop a low‐fat (LF) mayonnaise containing polysaccharide gums as functional ingredients. Xanthan gum (XG, 15 g kg−1), citrus fiber (CF, 100 g kg−1) and variable concentration of guar gum (GG) were used to formulate the optimum ratios of polysaccharide gums as fat replacers. The fat content in LF mayonnaise was reduced to 50% if compared with full‐fat (FF) mayonnaise, and the products still maintained ideal rheological properties.RESULTS: The rheological parameters showed that there were no (P > 0.05) differences in yield stress, viscosity and flow behavior index between XG + 10 g kg−1 GG, CF + 5 g kg−1 GG and FF control. LF mayonnaises had lower caloric values and higher dietary fiber content than the FF counterpart. Scanning electron microscopy (SEM) micrographs illustrated that the network of aggregated droplets in LF treatments contained a large number of interspaced voids of varying dimensions. Furthermore, in a comparison of sensory evaluation of LF treatments with commercial and our FF mayonnaises, there were no (P > 0.05) differences in any sensory scores among XG + 10 g kg−1 GG control.CONCLUSION: This study shows that XG + 10 g kg−1 GG and CF + 5 g kg−1 GG could be used in LF mayonnaise formulations based on its multiple functions on processing properties. Copyright © 2010 Society of Chemical Industry