bluuebunny/crossref_metadata_2025_split huggingface dataset:
How general practitioners treat migraine patients—evaluation of a headache guideline
Authors: Zielman R , Veenstra PJL , Zwet EW van , Berg JSP van den 
Published: July 2012

Aim: The aim of the study was to evaluate the pharmacological treatment of migraine patients by general practitioners before referral to a neurologist. First, was the pharmacological treatment in accordance with the Dutch College of General Practitioners headache guideline? Second, which migraine characteristics were associated with receiving migraine-specific medication? Methods: Migraine patients (age ≥18 years) who visited the neurology outpatient clinic for the first time were included. Migraine characteristics and pharmacological status were collected retrospectively for each patient from the general practitioner’s referral letter, hospital record and a headache characteristics questionnaire. Results: A total of 420 migraine patients were included. Only 18.3% of the patients with two or more migraine attacks per month were using prophylactic medication. Furthermore, only 11.7% of patients with symptoms of nausea and/or vomiting were using anti-emetic medication. More than half of patients (51.7%) were using triptans and were likely to have typical migraine symptoms and a long history of migraine. Conclusions: Migraine prophylactic medication and anti-emetics are underutilized in the primary care setting for migraine patients in the Netherlands, when compared to the general practitioners guideline. It is important to enhance the knowledge of general practitioners regarding the diagnosis of migraine, and to increase awareness of the headache guideline.
Arthroscopic Treatment of Rotator Cuff Tears With Shoulder Stiffness
Authors: Chuang Tai-Yuan , Ho Wei-Pin , Chen Chih-Hwa , Lee Chian-Her , Liau Jiann-Jong , Huang Chang-Hung 
Published: July 2012

Background: In a single arthroscopic surgery for rotator cuff tears with a stiff shoulder, closed manipulation with or without arthroscopic capsular release has been combined with the repair. Few studies have reported functional results after this surgery, and a clinical comparison has not been reported. Purpose: To compare functional outcomes with and without capsular release in arthroscopic treatment of rotator cuff tears with a stiff shoulder. Study Design: Cohort study; Level of evidence, 3. Methods: Among patients who underwent arthroscopic rotator cuff repairs with a concomitant stiff shoulder, 34 patients were treated with either manipulation and arthroscopic capsular release (group A; n = 18) or manipulation without capsular release (group B; n = 16). Preoperative mean passive forward flexion was 118° in group A and 117° in group B, whereas external rotation at the side was 28° and 29°, respectively. All patients were evaluated at a minimum 2-year follow-up in terms of visual analog scale for pain, muscle power, range of motion, Constant score, modified American Shoulder and Elbow Surgeons (ASES) shoulder evaluation form, and modified University of California at Los Angeles (UCLA) scores. Results: The mean modified ASES score was 90.3 for group A and 87.7 for group B ( P = .086). The mean UCLA scores were 34.3 and 33.0, respectively ( P = .247). The 2 groups showed a significant difference in forward flexion and external rotation post operatively, as group B recovered more slowly. There was no difference in the mean visual analog scale for pain at the last follow-up, but a 13° difference in forward flexion ( P < .001) and a 10° difference in external rotation ( P =.001) were seen. Conclusion: Overall satisfactory results could be achieved by either method, whereas rapid recovery and improvement of range of motion could be achieved by using a single arthroscopic repair and concomitant release for patients with rotator cuff tears with stiff shoulder.
Histological Assessment of Mechanoreceptors in Achilles Allografts After Anterior Cruciate Ligament Reconstruction
Authors: Kim Sung Hun , Chun Churl Hong , Chun Keun Churl , Jo Hyang Jeong , Kim Kwang Mee 
Published: July 2012

Background: There is a lack of histological studies investigating the presence of mechanoreceptors in anterior cruciate ligament (ACL) allografts. Hypothesis: Mechanoreceptors would not grow in Achilles allografts after ACL reconstruction. Study Design: Case series study; Level of evidence, 4. Methods: Tissue samples were obtained from 11 patients who underwent ACL reconstruction using Achilles tendon allografts. They underwent biopsies during second-look arthroscopies. The mean period from ACL reconstruction to harvesting tissue was 26.63 months (range, 12-120 months). The control group consisted of 2 normal ACLs procured from 42- and 45-year-old men who underwent amputation above the knee due to trauma. Results: Ruffini corpuscles and free nerve endings were shown to be present in the specimens of the control group by processing hematoxylin-eosin stains and immunohistochemical stains with monoclonal antibodies against S-100. In the Achilles allografts, mechanoreceptors were not observed. However, fibroblasts, collagen fibers, and vessels that were not present in fresh-frozen Achilles allografts before surgery were observed. Conclusion: The results demonstrate that Achilles tendon allografts appeared similar to normal ligaments except for the lack of histological evidence of mechanoreceptors. In other words, there are no newly ingrown mechanoreceptors in ACL allografts.
Effect of Fatigue on Tibial Rotation After Single- and Double-Bundle Anterior Cruciate Ligament Reconstruction
Authors: Hantes Michael E. , Tsarouhas Alexandros , Giakas Giannis , Spiropoulos Giannis , Sideris Vasilios , Christel Pascal , Malizos Konstantinos N. 
Published: July 2012

Background: Fatigue is an extrinsic factor adversely affecting joint proprioception and neuromuscular response, thereby increasing anterior cruciate ligament (ACL) strain and injury risk. The effectiveness of the single- and double-bundle techniques for ACL reconstruction to control residual rotational knee laxity under fatigue has not been examined. Hypothesis: Fatigue results in a significant increase in tibial rotation angles and moments in both ACL-intact and single- and double-bundle ACL-reconstructed knees. The 2 groups with ACL-reconstructed knees will show no significant differences in tibial rotation angles and moments either pre- or postfatigue. Study Design: Controlled laboratory study. Methods: Twenty-four male patients who underwent successful single-bundle (n = 12) or double-bundle (n = 12) ACL reconstructions and 10 matched healthy controls were subjected to a standard lower limb muscle fatigue protocol using an isokinetic dynamometer. Three-dimensional motion analysis was used to measure tibial rotation and rotational knee moments in the pre- and postfatigue states, during a swinging maneuver on the weightbearing leg from a standing position with the knee in extension. Results: Tibial rotation of the single-bundle group significantly increased postfatigue (prefatigue 22° ± 10° vs 29° ± 15° postfatigue, P = .015). In contrast, the double-bundle group showed similar tibial rotation values pre- and postfatigue (16° ± 6° vs 18° ± 4°, P = .22). The double-bundle group showed a trend toward decreased tibial rotation values pre- and post-fatigue compared with controls (22 ± 4 and 23 ± 4) ( P = .065 and .08, respectively). In the prefatigue state, rotational moments (N·mm/Kg) of the single-bundle (339 ± 148) and double-bundle (317 ± 97) groups were significantly lower than that of controls (465 ± 134) ( P = .05 and .03, respectively). In the postfatigue state, an increase was observed in rotational moments of the single-bundle (388 ± 131) and double-bundle (408 ± 187) groups compared with prefatigue values, whereas a decrease was noted in the control group (411 ± 117). Conclusion: Single-bundle ACL-reconstructed knees demonstrate a reduced ability to resist rotational loads under fatigue. Double-bundle reconstructed knees had significantly better control of tibial rotation when fatigued. However, they demonstrate an excessive, yet not significant, reduction in tibial rotation compared with the intact knee, suggesting a possible overcorrection in rotational laxity.
Efficacy and safety of NVA237versusplacebo and tiotropium in patients with COPD: the GLOW2 study
Authors: Kerwin Edward , Hébert Jacques , Gallagher Nicola , Martin Carmen , Overend Tim , Alagappan Vijay K.T. , Lu Yimeng , Banerji Donald 
Published: July 2012

NVA237 (glycopyrronium bromide) is a once-daily long-acting muscarinic antagonist (LAMA) in development for chronic obstructive pulmonary disease (COPD). The GLycopyrronium bromide in COPD airWays clinical Study 2 (GLOW2) evaluated the efficacy and safety of NVA237 in moderate-to-severe COPD over 52 weeks.Patients were randomised 2:1:1 to NVA237 50 μg, placebo or open-label tiotropium 18 μg for 52 weeks. Primary end-point was trough forced expiratory volume in 1 s (FEV1) at 12 weeks.1,066 patients were randomised, 810 completed the study. At week 12, trough FEV1increased significantly by 97 mL with NVA237 (95% CI 64.6–130.2; p<0.001) and 83 mL with tiotropium (95% CI 45.6–121.4; p<0.001). Compared with placebo, NVA237 produced significant improvements in dyspnoea (Transition Dyspnoea Index at week 26; p=0.002) and health status (St George's Respiratory Questionnaire at week 52; p<0.001). NVA237 significantly reduced the risk of moderate-to-severe COPD exacerbations by 34% (p=0.001) and the use of rescue medication (p=0.039),versusplacebo. NVA237-placebo and tiotropium-placebo differences were comparable for all outcomes. Safety profiles were similar across groups.NVA237 50 μg provided significant improvements in lung function, dyspnoea, health status, exacerbations and rescue medication use,versusplacebo, and was comparable to tiotropium. NVA237 can potentially be an alternative choice of LAMA for COPD patients.
Low miR-145 and high miR-367 are associated with unfavourable prognosis in resected nonsmall cell lung cancer
Authors: Campayo Marc , Navarro Alfons , Viñolas Nuria , Diaz Tania , Tejero Rut , Gimferrer Josep M. , Molins Laureano , Cabanas Maria L. , Ramirez Jose , Monzo Mariano , Marrades Ramon 
Published: July 2012

The transcription factors SRY-related HMG box (SOX)2 and octamer-binding transcription factor (OCT)4 regulate the expression of the miR-302–367 cluster. miR-145 regulates SOX2 and OCT4 translation and p53 regulates miR-145 expression. We analysed the expression of the miR-302–367 cluster and miR-145 and the mutational status of p53 in resected nonsmall cell lung cancer (NSCLC) patients and correlated results with time to relapse (TTR).Tumour and paired normal tissue samples were obtained from 70 NSCLC patients. MicroRNA expression was assessed with TaqMan MicroRNA Assays. p53 exons 5 to 8 were sequenced.miR-145 was downregulated (p<0.0001) and miR-367 was upregulated (p<0.0001) in tumour compared with normal tissue. Mean TTR was 18.4 months for patients with low miR-145 levels and 28.2 months for those with high levels (p=0.015). Mean TTR was 29.1 months for patients with low miR-367 levels and 23.4 months for those with high levels (p=0.048). TTR was shorter for patients with both unfavourable variables (p=0.009). Low miR-145 expression (p=0.049), the combination of unfavourable microRNA levels (p=0.02) and the combination of low miR-145 with p53 mutations (p=0.011) were independent markers of shorter TTR.In conclusion, miR-145 and miR-367 expression could be novel markers for relapse in surgically treated NSCLC. p53 may play a role in modulating miR-145 expression in NSCLC.
Simvastatin suppresses RANTES-mediated neutrophilia in polyinosinic–polycytidylic acid-induced pneumonia
Authors: Lee Chang Seok , Yi Eun Hee , Lee Jin-Ku , Won Cheolhee , Lee Young Ju , Shin Min Kyung , Yang Young Mok , Chung Myung-Hee , Lee Jung Weon , Sung Sang-Hyun , Ye Sang-Kyu 
Published: July 2012

Recently, statins have been shown to have anti-inflammatory effects on lung inflammatory diseases. However, the mechanisms of action of simvastatin in viral pneumonia have yet to be elucidated, although viral infection remains a considerable health threat. In this study, we hypothesised that simvastatin inhibits polyinosinic–polycytidylic acid (poly I:C)-induced airway inflammation, such as RANTES (regulated on activation, normal T-cell expressed and secreted) expression and inflammatory cell recruitment.In bronchial cells, the effect of simvastatin on poly I:C-induced RANTES expression and signal transducer and activator of transcription (STAT)3-mediated signal transduction was determined using an ELISA and short hairpin (sh)RNA system. In a poly I:C-induced pneumonia mouse model, immunological changes in the lungs after simvastatin inhalation, such as inflammatory cell recruitment and cytokine/chemokine release, were examined.In poly I:C-stimulated bronchial cells, RANTES secretion was increased by STAT3 activation, and simvastatin suppressed poly I:C-induced STAT3 activation, resulting in inhibition of RANTES expression. In BALB/c mice stimulated with inhaled poly I:C, RANTES expression and neutrophil infiltration into the airway were elevated. However, simvastatin treatment attenuated STAT3 activation, RANTES release and subsequent neutrophilia in the lungs.These findings suggest that simvastatin inhibits airway inflammation, but there are other mechanisms that need to be fully elucidated.
Prognostic factors in resected pathological N1-stage II nonsmall cell lung cancer
Authors: Liu Chao-Yu , Hung Jung-Jyh , Wang Bing-Yen , Hsu Wen-Hu , Wu Yu-Chung 
Published: July 2012

Stage II nonsmall cell lung cancer (NSCLC) has been redefined in the seventh edition of tumour, node, metastasis (TNM) classification for lung cancer. Stages IIa and IIb both contain node-negative (N0) and node-positive (N1) subgroups. The aim of this study was to evaluate the prognostic factors for overall survival in patients with resected N1-stage II NSCLC.Between January 1992 and December 2010, we retrospectively reviewed the clinicopathological characteristics of 163 N1-stage II (T1a-T2bN1M0) NSCLC in patients undergoing curative resection as primary treatment.Median follow-up time was 37.2 months. The 1-, 3- and 5-yr overall survival rates were 85.3%, 62.1% and 43.5%, respectively. Tumour involvement of the hilar/interlobar nodal zone and poorly differentiated histological grade were significant predictors for worse overall survival using multivariate analysis (p = 0.001 and p = 0.015, respectively). There were trends toward worse overall survival in older patients and those with larger tumour size (p = 0.063 and p = 0.075, respectively).In resected N1-stage II NSCLC, hilar/interlobar nodal involvement and poorly differentiated histological grade were significant predictors of worse overall survival. The differences in survival between these subgroups of patients may lead to the use of different adjuvant therapies or post-surgical follow-up strategies.
Insomnia related to sleep apnoea: effect of long-term auto-adjusting positive airway pressure treatment
Authors: Nguyên Xuân-Lan , Rakotonanahary Dominique , Chaskalovic Joël , Fleury Bernard 
Published: July 2012

Insomnia related to sleep apnoea (IA) is highly prevalent, and its proper treatment is still debated. The aim of this study was to test the hypothesis that long-term auto-adjusting positive airway pressure (APAP) treatment improves IA symptoms.80 patients (mean±sdage 54.9±10.6 yrs, respiratory disturbance index (RDI) 45.0±24.6 events·h−1) receiving APAP treatment were followed prospectively for 24 months. Somnolence and depression were assessed at baseline (T0) with the Epworth and the QD2A scales, respectively. Nightly APAP use was measured after 24 months of treatment (T24). The assessment of insomnia at T0 and T24 used the insomnia severity index (ISI). The combination of ISIT0≥15 and ISIT0–ISIT24≥9 defined the APAP-responding insomnia (APAP-RI) group. A logistic regression analysis identified the factors independently associated with the APAP-RI group.The ISI (13.7±5.7versus8.2±6.3) decreased significantly from T0 to T24 (p = 0.0001) for the patients as a whole, with a mean decrease of 13.5±2.9. Among the 39 insomniac subjects (T0), 20 belonged to the APAP-RI group (51%). The Epworth score (OR 1.536, 95% CI 1.093–2.159; p = 0.01) and the RDI (OR 1.080, 95% CI 1.010–1.154; p = 0.02) increased the risk of belonging to the APAP-RI group.IA symptoms improved with APAP treatment, and improvement was associated with the initial severity of the disease and somnolence in our population.
Quality of life and healthcare utilisation in cystic fibrosis: a multicentre study
Authors: Bradley Judy M. , Blume Steven W. , Balp Maria-Magdalena , Honeybourne David , Elborn J. Stuart 
Published: July 2012

The aim of our study was to discover the health status and healthcare utilisation associated with pulmonary exacerbations in cystic fibrosis (CF) and chronicPseudomonas aeruginosainfection.Patients with CF from five UK CF centres attended two visits, 8–12 weeks apart. They were classified at visit 1 as being in one of the three health states: no current pulmonary exacerbation; “mild” (no hospitalisation) pulmonary exacerbation; and “severe” (hospitalisation) pulmonary exacerbation. All patients completed the Cystic Fibrosis Questionnaire-Revised (CFQ-R) and EuroQol (EQ-5D) and a clinical form, and forced expiratory volume in 1 s (FEV1) was measured at visits 1 and 2. Annual healthcare utilisation data were collected.94 patients of mean±sdage 28.5±8.2 yrs and FEV158.7±26.8% were recruited. 60 patients had no pulmonary exacerbation, 15 had a mild and 19 had a severe pulmonary exacerbation at visit 1. EQ-5D and CFQ-R data showed that the worse the exacerbation, the poorer the health-related quality of life (HRQoL). There were strong relationships between the CFQ-R and EQ-5D domain scores. The mean rate of pulmonary exacerbations per patient per year was 3.6 (1.5 in hospital and 2.2 at home). The mean length of stay per hospital pulmonary exacerbation was 9 days.As exacerbation status worsens, patients experience worse HRQoL. There is a significant healthcare burden associated with treatment of pulmonary exacerbation and long-term prophylaxis.
Effectiveness of mesothelin family proteins and osteopontin for malignant mesothelioma
Authors: Pantazopoulos Ioannis , Boura Paraskevi , Xanthos Theodoros , Syrigos Konstantinos 
Published: July 2012

Malignant mesothelioma (MM) is an aggressive tumour with poor prognosis whose early diagnosis is difficult. Mesothelin, megakaryocyte potentiating factor (MPF) and osteopontin have attracted attention as biomarkers. The aim of the present review is to provide an overview regarding these candidate biomarkers for MM, and discuss their potential role in today's clinical practice.Mesothelin and MPF have good specificity but sub-optimal sensitivity for detection of MM, being negative both in the sarcomatoid histological sub-type and in almost half of epithelioid mesothelioma, especially in the early stages. Osteopontin is a marker of the duration of asbestos exposure, but lacks specificity for mesothelioma. Several patient characteristics influence the diagnostic accuracy of biomarkers and make the establishment of the “optimal” diagnostic threshold difficult. Mesothelin and MPF have proved useful in assessing response to treatment. Combining different markers together may lead to an improvement in diagnostic accuracy, but there is still need for research in this area. Extensive validation and further research is required to improve the use of serum markers in mesothelioma management. In the near future, their application in clinical practice is probably in monitoring response to therapy, rather than in guiding diagnostic decisions and risk assessment of asbestos-exposed populations.
Common ALDH2 genetic variants predict development of hypertension in the SAPPHIRe prospective cohort: Gene-environmental interaction with alcohol consumption
Authors: Chang Yi-Cheng , Chiu Yen-Feng , Lee I-Te , Ho Low-Tone , Hung Yi-Jen , Hsiung Chao A , Quertermous Thomas , Donlon Timothy , Lee Wei-Jei , Lee Po-Chu , Chen Che-Hong , Mochly-Rosen Daria , Chuang Lee-Ming 
Published: July 2012

Abstract

Background
Genetic variants near/within the ALDH2 gene encoding the mitochondrial aldehyde dehydrogenase 2 have been associated with blood pressure and hypertension in several case–control association studies in East Asian populations.

Methods
Three common tag single nucleotide polymorphisms (tagSNP) in the ALDH2 gene were genotyped in 1,134 subjects of Chinese origin from the Stanford Asia-Pacific Program for Hypertension and Insulin Resistance (SAPPHIRe) family cohort. We examined whether the ALDH2 SNP genotypes predicted the development of hypertension in the prospective SAPPHIRe cohort.

Results
Over an average follow-up period of 5.7 years, carriers homozygous for the rs2238152 T allele in the ALDH2 gene were more likely to progress to hypertension than were non-carriers (hazard ratio [HR], 2.88, 95% confidence interval [CI], 1.06-7.84, P = 0.03), corresponding to a population attributable risk of ~7.1%. The risk associated with the rs2238152 T allele were strongest in heavy/moderate alcohol drinkers and was reduced in non-drinkers, indicating an interaction between ALDH2 genetic variants and alcohol intake on the risk of hypertension (P for interaction = 0.04). The risk allele was associated with significantly lower ALDH2 gene expression levels in human adipose tissue.

Conclusion

ALDH2 genetic variants were associated with progression to hypertension in a prospective Chinese cohort. The association was modified by alcohol consumption.
A novel Kluyveromyces marxianus strain with an inducible flocculation phenotype
Authors: Vallejo Juan A , Serrat Manuel , Pérez-Portuondo Irasema , Sánchez-Pérez Angeles , Ageitos Jose M , Villa Tomas G 
Published: July 2012

Abstract
Flocculation is a very useful phenotype for industrial yeast strains, since it facilitates cell harvest and represents an easy way of cell immobilization in continuous fermentation processes. The present work represents the first time that an inducible flocculation phenotype has been generated in a non flocculent strain of Kluyveromyces marxianus. This was accomplished by expressing Saccharomyces cerevisiae FLO5 gene in K. marxianus CECT 11769 strain. The FLO 5 gene was placed under the control of an EPG promoter, not repressed by glucose and induced by anoxia. Our experimental approach successfully generated two novel K. marxianus flocculent phenotypes: one inducible and one constitutive. The constitutive phenotype originated from deletions in the FLO5 promoter region, indicating the existence of putative upstream repressor site involved in oxygen regulation of the EPG1 promoter. The novel strains here generated had a unique set of characteristics that provided an advantage, over the wild-type strain, for the industrial co-production of ethanol and polygalacturonase.