bluuebunny/crossref_metadata_2025_split huggingface dataset:
Technical aspects and clinical applications of measuring BCR‐ABL1 transcripts number in chronic myeloid leukemia
Authors: Foroni Letizia , Gerrard Gareth , Nna Emmanuel , Khorashad Jamshid Sorouri , Stevens David , Swale Bryony , Milojkovic Dragana , Reid Alistair , Goldman John , Marin David 
Published: May 2009

AbstractChronic myeloid leukemia (CML) is a myeloproliferative disorder characterized by a triphasic clinical course, the morphologic expansion of a terminally differentiated myeloid cell and the presence of the BCR‐ABL1 fusion gene, the hallmark of CML. The fusion gene is usually, but not always, associated with a Philadelphia chromosome, the result of a reciprocal exchange of genetic material between chromosome 22 and chromosome 9, which leads to the production of the activated BCR‐ABL1 gene and oncoprotein. The breakpoint in the BCR gene occurs commonly downstream of exons e13 or e14 (M‐BCR) and less frequently downstream of exons e1 and e2 (m‐BCR). Less than 1% of cases carry a breakpoint downstream of exon 6 or 8 (“variant fusion genes”) or exon 19 (μ‐BCR). Breakpoints in the ABL1 gene cluster upstream of exon a2 (or of exon a3 in less than 5% of patients with CML). Conventional cytogenetic, fluorescence in situ hybridization, and molecular testing for the BCR‐ABL1 fusion gene are key investigations for the diagnosis and monitoring of CML. Treatment using tyrosine kinase inhibitors has revolutionized the management of CML with hematologic and cytogenetic response within 12–18 months observed in >85% of patients. Nevertheless, between 15 and 20% of patients may evolve to blastic phase. Measurement of low level or “minimal” residual disease using molecular tests is becoming the gold‐standard approach to measure response to therapy due to its higher sensitivity compared to other routine techniques. The technical aspects and clinical applications of molecular monitoring will be the main focus of this article. Am. J. Hematol., 2009. © 2009 Wiley‐Liss, Inc.
From Metal Binding to Nanoparticle Formation: Monitoring Biomimetic Iron Oxide Synthesis within Protein Cages using Mass Spectrometry
Authors: Kang Sebyung , Jolley Craig C. , Liepold Lars O. , Young Mark , Douglas Trevor 
Published: May 2009

AbstractDie Bestimmung der Massen metallmineralisierter Proteinkäfige ermöglichte eine quantitative Untersuchung des Einflusses der Metallionenkonzentration auf die Größe der gebildeten Nanopartikel. Modellrechnungen mithilfe einer kinetischen Mastergleichung sprechen dafür, dass das Partikelwachstum aus einer Bindungs‐ und einer Wachstumsphase besteht. I: relativer Überschuss; LiDps: ein DNA‐bindendes Protein.magnified image
Phosphonium Salts as Chiral Phase‐Transfer Catalysts: Asymmetric Michael and Mannich Reactions of 3‐Aryloxindoles
Authors: He Rongjun , Ding Changhua , Maruoka Keiji 
Published: May 2009

AbstractChiraler PTC: Eine hoch effiziente asymmetrische Michael‐Addition von 3‐Aryloxindolen gelang mit einem Tetraalkylphosphoniumsalz als chiralem Phasentransferkatalysator (PTC). Die Produkte wurden in nahezu quantitativen Ausbeuten und mit hohenee‐Werten erhalten. Derselbe Katalysator vermittelte auch eine asymmetrische Mannich‐Reaktion von 3‐Aryloxindolen.magnified image
Oxidative Carbocation Formation in Macrocycles: Synthesis of the Neopeltolide Macrocycle
Authors: Tu Wangyang , Floreancig Paul E. 
Published: May 2009

AbstractMakrocyclische Oxocarbeniumionen wurden aus Makrolactonen mit Benzyl‐ oder Allyletherfunktionen durch oxidative C‐H‐Bindungsaktivierung in Gegenwart von 2,3‐Dichlor‐4,5‐dicyanchinon (DDQ) erhalten. Die Nützlichkeit dieser effizienten Reaktion für die Synthese komplexer Moleküle wurde anhand ihrer Verwendung zum Aufbau des Tetrahydropyronrings in einer kurzen Formalsynthese von Neopeltolid demonstriert (siehe Retrosynthese).magnified image
Interaction and Reaction of the Phenyl Radical with Water: A Source of OH Radicals
Authors: Mardyukov Artur , Sanchez‐Garcia Elsa , Crespo‐Otero Rachel , Sander Wolfram 
Published: May 2009

AbstractThat's radical! A photochemical reaction between the phenyl radical and water results in the abstraction of a hydrogen atom from water and the formation of a hydroxyl radical. The hydroxyl radical forms an OH⋅⋅⋅π hydrogen bond with benzene (see picture) and does not react with benzene thermally under the conditions of matrix isolation.magnified image
Coordination‐Driven Self‐Assembly of PEO‐Functionalized Perylene Bisimides: Supramolecular Diversity from a Limited Set of Molecular Building Blocks
Authors: Gebers Jan , Rolland Damien , Frauenrath Holger 
Published: May 2009

AbstractA limited number of poly(ethylene oxide)‐substituted perylene bisimides, some of which are equipped with terpyridine ligands for transition‐metal coordination (see structure), combine different types of noncovalent interactions to yield optoelectronically active organic materials with different types of supramolecular morphologies.magnified image
Mirror‐Image Photoswitching of Individual Single‐Walled Carbon Nanotube Transistors Coated with Titanium Dioxide
Authors: Liu Song , Li Jianming , Shen Qian , Cao Yang , Guo Xuefeng , Zhang Guoming , Feng Chaoqun , Zhang Jin , Liu Zhongfan , Steigerwald Michael L. , Xu Dongsheng , Nuckolls Colin 
Published: May 2009

AbstractDotting the i's: Stimuli‐responsive optoelectronic devices are formed from the title transistors functionalized with photoactive quantum dots. The p‐type semiconducting tubes show a fast current decrease under UV irradiation and reversibility when the UV irradiation is switched off. In contrast, ambipolar tubes show mirror‐image photoswitching effects when negative and positive gate bias voltages are applied.magnified image
Cover Picture: Selectivity Control in Gold‐Mediated Esterification of Methanol (Angew. Chem. Int. Ed. 23/2009)
Authors: Xu Bingjun , Liu Xiaoying , Haubrich Jan , Madix Robert J. , Friend Cynthia M. 
Published: May 2009

AbstractAtomic oxygen adsorbed on metallic gold promotes the low‐temperature transformation of methanol to methyl formate, formaldehyde, and formic acid. The reactions occur with oxygen‐containing gold nanoparticles (ca. 2 nm in diameter), which form when Au(111) is oxidized with ozone. The detailed reaction mechanism is discussed by C. M. Friend and co‐workers in the Communication on page 4206 ff.magnified image
Inside Cover: Matrix‐Free Formation of Gas‐Phase Biomolecular Ions by Soft Cluster‐Induced Desorption (Angew. Chem. Int. Ed. 23/2009)
Authors: Gebhardt Christoph. R. , Tomsic Anna , Schröder Hartmut , Dürr Michael , Kompa Karl L. 
Published: May 2009

AbstractMass spectrometry of biomolecules has developed into a key technology in bioanalytics. As described by C. R. Gebhardt, M. Dürr et al. in their Communication on page 4162 ff., surface collisions of neutral molecular clusters can be used for soft formation of the required gas‐phase biomolecular ions. Upon impact on a surface, the clusters provide the energy for desorption of the adsorbed biomolecules and serve as a transient matrix. Subsequent evaporative cooling prevents shattering and finally leads to an isolated biomolecular ion in the gas phase.magnified image
Hydrolysis of C,N‐chelated diorganotin(IV) chlorides and catalysis of transesterification reactions
Authors: Padělková Zdeňka , Weidlich Tomáš , Císařová Ivana , Růžička Aleš 
Published: May 2009

AbstractDiorganotin(IV) dichlorides of formula LCNRSnCl2 (where R is nBu or Ph) containing one LCN chelating ligand were hydrolyzed with aqueous sodium hydroxide in benzene. The composition of the products is strongly dependent on the amount of hydroxide. The partially hydrolyzed compounds of composition (LCNRSnCl)2(µ‐O) were isolated as crystalline products. A hydrolysis where more than one molar equivalent of NaOH is employed gave only a mixture of unidentifiable products. The structure of (LCNPhSnCl)2(µ‐O) was determined by X‐ray diffraction techniques in the solid state. In solution there was a mixture of diastereoisomers found, where the tin atoms serve as a stereogenic centers. The catalytic activity of starting dichlorides as well as (LCNPhSnCl)2(µ‐O) in various transesterification processes was investigated. The activity is very low in the case of starting dichlorides. When two molar equivalents of NaH are added or (LCNPhSnCl)2(µ‐O) is employed in the catalytic experiments, the activity is comparable to the literature data. Copyright © 2009 John Wiley & Sons, Ltd.
Regio‐ and stereospecific synthesis of polysubstituted alkenes by carbozincation of acetylenic sulfones
Authors: Xie Meihua , Gu Xiaoxia , Wang Jialiang , Zhang Jinhua , Lin Gaofeng , Wang Shaowu 
Published: May 2009

AbstractTri‐ or tetrasubstituted alkenes with various substituents can be constructed regio‐ and stereospecifically by treatment of acetylenic sulfones with organozinc reagents in tetrahydrofuran followed by hydrolysis or coupling with halohydrocarbon. Copyright © 2009 John Wiley & Sons, Ltd.
Reduced water intake: Implications for rodent developmental and reproductive toxicity studies
Authors: Campbell Marlissa A. , Golub Mari S. , Iyer Poorni , Kaufman Farla L. , Li Ling‐Hong , Moran Messen Francisco , Morgan James E. , Donald James M. 
Published: May 2009

AbstractIn developmental and reproductive toxicity studies, drinking water is a common means of delivering the test agent. Reduced consumption of toxicant‐containing water raises questions about indirect effects of reduced maternal fluid consumption resulting from unpalatability, versus direct effects of the test compound. Issues to consider include: objective assessment of dehydration and thirst, the relative contributions of innate and learned behaviors to drinking behavior and flavor preference, and the objective assessment of physiologic stress. Not only do lab animals under ad lib conditions consume more water than the minimum required to maintain fluid balance, animals faced with water restriction have substantial physiologic capacity for protection of metabolic processes. Measures of blood biochemistry can provide quantifiable, objective indications of fluid balance, but changes in these parameters could result from other causes such as effects of a test toxicant. Consummatory behaviors in response to perceived need are highly influenced by learning. Hence, the drinking behavior, water intake, and flavor acceptance/preference of animals used in toxicology experiments could be subject to learning experiences with the test compound. Physiological symptoms of stress produced by water deprivation may be distinguishable from the symptoms associated with other generalized stressors, such as food deprivation, but doing so may be beyond the scope of most developmental or reproductive toxicity studies. Use of concurrent controls, paired to test groups for water consumption, could help distinguish between the direct effects of a test toxicant as opposed to effects of reduced water consumption alone. Birth Defects Res (Part B), 86:157–175, 2009. ©2009 Wiley‐Liss, Inc.
Fluorescence enhancement of warfarin induced by interaction with β‐cyclodextrin
Authors: Vasquez Jacob M. , Vu Andrew , Schultz Jerome S. , Vullev Valentine I. 
Published: May 2009

AbstractWarfarin is the most common agent used for control and prevention of venous as well as arterial thromboembolism (blood clots). In aqueous media, warfarin forms inclusion complexes with a family of cyclic oligosaccharides, α, β, γ‐cyclodextrins (CD). The formation of these complexes results in enhancement of the fluorescence of warfarin. Such spectroscopic changes offer a venue for the development of bioanalytical methodologies for warfarin quantification in biological liquids. We characterized the photophysical properties of warfarin in solvents with varying polarity and viscosity. The fluorescence quantum yield of warfarin correlated: (1) strongly with the solvent viscosity (R = 0.979) and (2) weakly with the solvent polarity (R = 0.118). These findings indicate that it is the change of the viscosity, rather than polarity, of the microenvironment that causes the fluorescence enhancement of warfarin upon binding to β‐CD. Utilizing the observed fluorescence enhancement in fluorescence titration measurements, the binding constants of warfarin to β‐CD were obtained (2.6 × 102 M−1–3.7 × 102 M−1). Using multivariable linear analysis, we extracted the stoichiometry of warfarin‐β‐CD interaction (1:1). © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009
Successful percutaneous management of left main trunk occlusion during percutaneous aortic valve replacement
Authors: Kapadia Samir R. , Svensson Lars , Tuzcu E. Murat 
Published: May 2009

AbstractTranscatheter aortic valve replacement with balloon‐expandable and self‐expandable stent valves is available in Europe. The balloon‐expandable valve is currently being investigated in a randomized trial in the US. We report an uncommon complication of left main trunk occlusion with balloon‐expandable valve deployment and its successful percutaneous management. Left main trunk occlusion was immediately recognized when patient did not recover blood pressure after valve deployment. TandemHeart™ support was used to stabilize the patient immediately and left main trunk was successfully stented. Analysis of anatomy leading to this complication and potential measures to predict and prevent such a complication are discussed in this report. © 2009 Wiley‐Liss, Inc.
Interatrial shunt closure devices in patients with nickel allergy
Authors: Reddy Bhavananda T. , Patel Jeetendra B. , Powell Douglas L. , Michaels Andrew D. 
Published: May 2009

AbstractWe assessed outcomes in nickel allergic patients treated with percutaneous interatrial shunt device closure with the Helex device. Nickel toxicity has been well described in patients undergoing interatrial shunt closure with the Amplatzer device, which has a nitinol design. There have been no reports using Helex in nickel allergic patients. Ninety‐five consecutive patients underwent percutaneous interatrial shunt closure at a single US center by one operator. In those with possible nickel allergy, patch testing with the North American Contact Dermatitis Group standard series and a metal series was performed. The mean age was 48 ± 16 years (range 18‐81), 48% were male, 21 (22%) had atrial septal defect, and 74 (78%) had patent foramen ovale. Six patients had a positive skin test to nickel and underwent successful closure with Helex. Of the remaining 89 patients, 88 were closed with Amplatzer and one with Helex. All procedures were successful with no deaths, myocardial infarctions, strokes, or systemic emboli at six‐month followup. None of the Helex patients developed an allergic reaction, significant chest pain, or arrhythmia. Of those without pre‐procedural known nickel allergy, 12% had palpitations, 5% had atrial fibrillation, and 13% had chest pain. When compared with a published report that 89% of nickel‐allergic patients developing an allergic reaction to the Amplatzer or Premere device, Helex appeared far safer in nickel allergic patients (P < 0.001). In patients with nickel allergy, percutaneous interatrial shunt device closure with Helex device is safe, and is not associated with allergy to nickel. © 2009 Wiley‐Liss, Inc.
The risk of cardiac complications following noncardiac surgery in patients with drug eluting stents implanted at least six months before surgery
Authors: Assali Abid , Vaknin‐Assa Hana , Lev Eli , Bental Tamir , Ben‐Dor Itsik , Teplitsky Igal , Brosh David , Fuchs Shmuel , Eidelman Leonid , Battler Alexander , Kornowski Ran 
Published: May 2009

AbstractAims: Given the anecdotal reports and case series suggesting that drug‐eluting coronary stents [DES] may be still vulnerable to coronary thrombosis after six months, we sought to assess this risk in patients undergoing non‐cardiac surgery six months after stenting. Methods and Results: Linking the Rabin Medical Centre interventional cardiology database with its non‐cardiac surgical database, we identified 78 patients who underwent DES placement and subsequently [after six months] had noncardiac surgery [15‐vascular, 37‐ abdominal and genitourinary and 26‐others, excluding ophthalmic surgery]. Outcome measures included 30‐day rate of postoperative myocardial infarction (MI), DES‐related thrombosis, and cardiac mortality. Major adverse cardiac events [death and non‐fatal MI] occurred in 6 (7.7%) patients including 2 cardiac deaths (2.6%), 4 (5.1%) non‐fatal myocardial infarctions (MIs). Two patients (2.6%) sustained stent thrombosis [one patient had ‘definite’ and one ‘probable’ stent thrombosis]. All MIs [including stent thrombosis] occurred in the vascular and abdominal surgery group. Two of the MIs events occurred while the patients were on dual antiplatelet agents. In conclusions: Perioperative cardiac events during non cardiac surgery after six months of DES deployment still occur. These cardiac complications [not entirely prevented by continued dual antiplatelet agents] remain a matter of diagnostic and therapeutic challenge and concern. © 2009 Wiley‐Liss, Inc.
Contralateral transradial approach for carotid artery stenting: A feasibility study
Authors: Patel Tejas , Shah Sanjay , Ranjan Alok , Malhotra Hemant , Pancholy Samir , Coppola John 
Published: May 2009

AbstractBackground: Carotid artery stenting (CAS) has become an accepted modality of treatment for revascularization of the internal carotid artery (ICA). CAS from femoral approach has got wide acceptance, however, it can be problematic due to access site complication as well as technical difficulties related to peripheral vascular disease and/or anatomical variations of the aortic arch. Small feasibility studies of CAS through ipsilateral transradial approach have been described in the literature. The purpose of the present study is to evaluate the feasibility of contralateral transradial approach as an alternative approach for CAS. Methods: Twenty patients (mean age: 65 ± 5, 17 male) underwent CAS using contralateral transradial approach. All had a CA stenosis greater than 80%. The target common carotid artery (CCA) was initially cannulated via the contralateral radial artery using a 5F Simmons 1 diagnostic catheter or a 5F TIG diagnostic catheter, which was then advanced to the external CA (ECA) over an exchange length of 0.032″ Terumo Glidewire or a 0.025″ Glidewire. Once the catheter was parked in the optimal position in ECA, the wire was removed and was replaced by 0.035″ Amplatz Super stiff Guide wire. Following that, the Simmons 1 or the TIG catheter was removed and 6F Pinnacle Sheath was exchanged and positioned in the distal CCA. CAS was performed using standard techniques with weight‐based heparin for anticoagulation. Results: CAS was successful in 16/20 (80%) patients, including 12/12 (100%) right CA, 4/8 (50%) left CA. Mean interventional time was 40 ± 5 min. The sheath was removed immediately after the procedure. There were no radial access site complications. One patient sustained a transient ischemic attack and recovered completely with complete resolution of symptoms within 1 hr. Median Hospital stay was 3 ± 0.5 days. Angulation of left CCA with the aortic arch was the technical cause of failure in the four unsuccessful cases. Conclusion: CAS using the contralateral transradial approach appears to be safe and technically feasible. The technique may be particularly useful in patients with right ICA lesions because of the favorable right CCA angle with the aortic arch. © 2009 Wiley‐Liss, Inc.
The impact of micro troponin leak on long‐term outcomes following elective percutaneous coronary intervention
Authors: Milani Richard V. , Fitzgerald Robert , Milani Jenna N. , Lavie Carl J. 
Published: May 2009

AbstractObjective: To evaluate the clinical impact of microleaks of troponin, which are below the reference standard defining troponin elevation, on cardiovascular outcomes in stable coronary patients undergoing elective percutaneous coronary intervention (PCI). Background: Troponin elevation, either pre‐ or post‐PCI, has been shown to predict poor cardiovascular outcomes. However, troponin measurements that are above the limit of detection but below the 99th percentile limit defining elevation (“microleak”) have uncertain clinical significance. Methods: We assessed subsequent myocardial infarction (MI) and death over a mean follow‐up of 4.2 years in 2,272 patients undergoing elective PCI, where baseline troponins were normal and follow‐up troponins were obtained 12–24 hr post‐PCI. Patients were divided into three groups based on post‐PCI troponin levels: Group 1 (n = 1,313) nondetectable; group 2 (n = 587) microleak, and group 3 (n = 372) elevated suggesting myocardial necrosis. Results: The combined endpoint of MI and death was similar in groups 2 and 3 (50.3 vs. 51.9%, respectively, P = NS), which was significantly more than group 1 patients (35.6%, P < 0.01) over the follow‐up period. Multivariate analysis of patients in groups 1 and 2 demonstrated that troponin microleak was an independent predictor of MI and death (P = 0.01). Conclusions: Microleak of troponin following elective PCI suggests myocardial injury and predicts an increased risk of subsequent MI and death. Troponins should be routinely assessed following PCI, and preventive therapies are needed to reduce micro and macro troponin elevation in the PCI setting. © 2009 Wiley‐Liss, Inc.
Cover Picture: Polymerase‐Catalysed Incorporation of Glucose Nucleotides into a DNA Duplex (Chem. Eur. J. 22/2009)
Authors: Renders Marleen , Abramov Mikhail , Froeyen Matheus , Herdewijn Piet 
Published: May 2009

AbstractSurprise, surprise DNA polymerases may accept glucose nucleotides as substrates. This unexpected broad substrate specificity of polymerases points to its role in the catalytic process of phosphodiester formation, rather than being involved in base pair recognition. In their Full Paper on page 5463 ff., P. Herdewijn et al. describe their findings in detail.magnified image
Organic carbonates as alternative solvents for asymmetric hydrogenation
Authors: Schäffner Benjamin , Andrushko Vasyl , Bayardon Jerome , Holz Jens , Börner Armin 
Published: May 2009

AbstractOrganic carbonates like propylene carbonate (PC) or butylene carbonate (BC) belong to the class of aprotic, highly dipolar solvents (AHD). Interestingly, their potential as solvents for asymmetric catalysis has been overlooked for a long time. The aim of this work is to evaluate organic carbonates and other organic solvents like THF, CH2Cl2, and acetonitrile as well as members of the AHD‐family (DMF, DMSO, etc.) as media for homogeneous asymmetric hydrogenation. For this reason cationic Rh‐complexes based on chiral phosphine ligands were tested in the hydrogenation of typical benchmark substrates. In several trials, significant advantages of organic carbonates were found. In contrast to DMSO, DMF and acetonitrile, in PC and BC high conversion rates and excellent enantioselectivities were usually observed. Chirality 2009. © 2009 Wiley‐Liss, Inc.