bluuebunny/crossref_metadata_2025_split huggingface dataset:
Claudin k is specifically expressed in cells that form myelin during development of the nervous system and regeneration of the optic nerve in adult zebrafish
Authors: Münzel Eva Jolanda , Schaefer Karin , Obirei Barbara , Kremmer Elisabeth , Burton Edward A. , Kuscha Veronika , Becker Catherina G. , Brösamle Christian , Williams Anna , Becker Thomas 
Published: October 2011

AbstractThe zebrafish has become an important model organism to study myelination during development and after a lesion of the adult central nervous system (CNS). Here, we identify Claudin k as a myelin‐associated protein in zebrafish and determine its localization during development and adult optic nerve regeneration. We find Claudin k in subcellular compartments consistent with location in autotypic tight junctions of oligodendrocytes and myelinating Schwann cells. Expression starts in the hindbrain at 2 days (mRNA) and 3 days (protein) postfertilization and is maintained in adults. A newly generated claudin k:green fluorescent protein (GFP) reporter line allowed us to characterize oligodendrocytes in the adult retina that express Claudin k and olig2, but not P0 and uniquely only form loose wraps of membrane around axons. After a crush of the adult optic nerve, Claudin k protein levels were first reduced and then recovered within 4 weeks postlesion, concomitant with optic nerve myelin de‐ and regeneration. During optic nerve regeneration, oligodendrocytes, many of which were newly generated, repopulated the lesion site and exhibited increasing morphological complexity over time. Thus, Claudin k is a novel myelin‐associated protein expressed by oligodendrocytes and Schwann cells from early stages of wrapping and myelin formation in zebrafish development and adult regeneration, suggesting important functions of the gene for myelin formation and maintenance. Our Claudin k antibodies and claudin k:GFP reporter line represent excellent ways to visualize oligodendrocyte and Schwann cell differentiation in vivo. © 2011 Wiley Periodicals, Inc.
Diagnostic accuracy of a new instrument for detecting cognitive dysfunction
Authors: Cercy Steven P. 
Published: October 2011

BackgroundEffective screening of cognitive disorders is limited by psychometric and practical properties of available instruments. The Brief Cognitive Screen (BCS) was developed to improve sensitivity and overall diagnostic accuracy of case detection for cognitive dysfunction in a quick and convenient format.MethodsThis validation study compared psychometric properties of the BCS with the Mini mental state examination (MMSE) in 308 patients with suspected or known disorders of cognition. Diagnostic accuracy of the MMSE was determined using the traditional 23/24 clinical cutoff and area under the curve (AUC) from receiver operating characteristics (ROC) analyses. Diagnostic accuracy of BCS summary scores and components was determined by ROC analyses. Comprehensive neuropsychological evaluation served as the criterion standard for determining cognitive dysfunction.ResultsThe MMSE sensitivity (34.8%) was inadequate at the 23/24 cutoff. Under ROC analyses, diagnostic accuracy of a BCS summary score was superior (AUC = 0.950) to the MMSE total score (AUC = 0.862) for the total sample. Excluding all patients with a history of cognitive deficit, classification accuracy of the BCS remained high (AUC = 0.936).ConclusionsFindings suggest that BCS is an effective, convenient alternative screening instrument for use in clinical populations with suspected cognitive dysfunction. Copyright © 2011 John Wiley & Sons, Ltd.
Experimental Investigation To Study the Influence of Handle Diameter on Low‐Frequency, Hand–Arm Vertical Vibration
Authors: Alphin Masilamany Santha , Sankaranarayanasamy Krishnaswamy , Sivapirakasam Suthangathan Paramashivan 
Published: October 2011

AbstractHandle vibration from equipment or machines influences musculoskeletal activity as well as comfort in handling the same. New technology can be worse than no technology if it was not developed correctly as ergonomic research has clearly demonstrated the relationship between injury risk and poorly designed hand tools. Clinical and epidemiological studies have shown that operators of handheld power tools are prone to develop various vibration‐induced disorders of the hand and arm, which are collectively referred to as “hand–arm vibration syndrome.'' The vibration direction has a great influence on the transmitted vibration. The present study focuses the effects of low‐frequency vertical vibration on hand to shoulder from handles of different size. The electrodynamic exciter is used for simulating vibration to a vertical handles of four different diameters. PULSE LabShop software is used for evaluating the magnitude of vibration in different frequency bands. The vibration characteristic data were acquired in the yh axis at the wrist, elbow, and shoulder for bent arm and extended arm postures with vibration excitation of 4.5 m/s2. Transmissibility characteristics are computed to determine the influence of handle diameter in yh vibration transmitted to the hand–arm system. The magnitude of vibration transmitted within the hand, elbow, and shoulder was observed to be dependent on the handle size; larger handles cause higher vibration transmissibility. The results also show that the human hand–arm system in an extended arm posture amplifies the vibration transmitted than bent arm in a small difference. © 2011 Wiley Periodicals, Inc.
Ergonomic Analysis of Head‐Mounted Night Vision Goggle Systems in Simulated Ground Operations
Authors: Tai Hung‐Sheng , Lee Yung‐Hui , Liu Bor‐Shong , Kuo Cheng‐Lang 
Published: October 2011

AbstractThis study compared the mounting time and strain on the head‐neck while using an old model (forehead and cheek supported) and a new model (head harness supported) of head‐mounted night vision goggle systems in the postures of standing, sitting, and lying prone. The results showed that effectively reducing the mounting time was attributed to the ease and less time required for length adjustments of the head harness in the new model. The novel device received higher positive subjective ratings for convenience and comfort while wearing than did the old model. Although the weight of the new model was decreased to 0.794 kg and the length was reduced to 0.155 m, the loads on the head‐neck complex remained high. These findings suggest that a rifle should be fitted with the night vision goggle or handheld model to decrease neck‐shoulder workload if the prone position is the most frequent and important posture for soldiers. © 2011 Wiley Periodicals, Inc.
Work Environment and the Bottom Line: Survey of Tools Relating Work Environment to Business Results
Authors: Rose Linda Maria , Orrenius Ulf Erik , Neumann Walther Patrick 
Published: October 2011

AbstractInsufficient knowledge of how the working environment (WE) impacts company performance leads to a poor basis for managerial decision making. The objective of this study was to survey and evaluate available tools relating WE to business results. Nine tools were identified and qualitatively evaluated using 11 criteria. The tools were clustered into three categories, ranging from analysis of WE risks as well as calculation of their economic impact to solely investment analysis. Shortcomings in the tools include insufficient attention to quality and productivity issues and insufficient guidance for users. Although further methodological development is indicated, researchers should also attend to the factors affecting tool uptake and application as well as the potential to integrate such analysis into companies' regular accounting systems. © 2011 Wiley Periodicals, Inc.
Contributions of imaging spectroscopy to improve estimates of evapotranspiration
Authors: Rodriguez J. M. , Ustin S. L. , Riaño D. 
Published: October 2011

AbstractImproved estimates of evapotranspiration (ET) are needed for water resource management and irrigation scheduling. We review the use of imaging spectroscopy to capture estimates of water vapour flux and biophysical components of ET. Remote sensing has long attempted to quantify and predict ET, with most applications relying only on green vegetation indexes from multispectral imagers combined with thermal radiance and weather data. In contrast, imaging spectrometry is an advanced remote sensing technology that measures hundreds of spectral bands in the solar spectrum. Plant pigments, water, and dry matter have unique spectral signatures that can advance estimates of ET and detection of drought stress. This allows analyses based on the physics of spectroscopy and avoids a requirement for continual empirical calibration. These spectral components provide unprecedented information about plant physiological processes, which improve understanding of the regulation of water fluxes and the energy budget. Laboratory, field, and airborne studies of spectral properties in the near‐ and shortwave‐infrared region show strong relationships with plant water relations like water content, relative water content, and water potential. Because water absorption features are spectrally independent of pigment absorptions in the visible region, they provide a new source of information about environmental conditions. These new observations from imaging spectroscopy will lead to better understanding of ecological and hydrological processes. Copyright © 2011 John Wiley & Sons, Ltd.
An infant who was born with a life‐threatening skin disease: Various aspects of triadic psychotherapy
Authors: Keren Miri 
Published: October 2011

AbstractA longitudinal case study of a 3‐year‐old toddler, born with a congenital, severe, life‐threatening skin disease, with comorbid diagnoses of failure to thrive and feeding disorder, is described and discussed. The referral, diagnostic, and therapeutic processes are described, and the main themes of the treatment sessions are presented in the associative order that they occurred. The understanding of the case is very much based on the “me‐skin'' concept. We have tried, through this case, to show the ways that we work with multidisciplinary team countertransferential reactions to the young child and her parents, the role that the child psychiatrist may take, and the special difficulty in working with cases that raise fear of death in parents as well as in therapists. We raise existential questions about worth of life, infant's will to grow, and infant's drive to live forever a painful life.
A SIMPLIFIED MULTI‐OBJECTIVE GENETIC ALGORITHM OPTIMIZATION MODEL FOR CANAL SCHEDULING
Authors: Peng S. Z. , Wang Y. , Khan S. , Rana T. , Luo Y. F. 
Published: October 2011

ABSTRACTA simplified Multi‐Objective Genetic Algorithm Optimization Model (MOM‐GA) for canal scheduling under unequal flow rates of distributary canals is presented in this paper. This MOM‐GA was designed for dynamic rotational scheduling with two objectives: to reduce fluctuations of flow rates of superior canals, and to reduce seepage losses of canal systems. This model was programmed in MATLAB using its genetic algorithm functions. Application of this model was demonstrated with a case study of the Nanguan Main Canal system (NMC) in the Gaoyou Irrigation Area, China. The results demonstrated that the MOM‐GA is an effective model for optimizing canal scheduling. NMC keeps running under a relatively steady range, and the seepage losses are reduced by around half that under current and binary optimized scheduling. The MOM‐GA is also sufficiently flexible to be applied to different levels in canal systems as a simplified approach for canal scheduling design and operation. The optimization results given by MOM‐GA can assist irrigators to make better canal scheduling decisions in each irrigation event. Copyright © 2011 John Wiley & Sons, Ltd.
Development of morphology and function of neonatal mouse ventricular myocytes cultured on a hyaluronan‐based polymer scaffold
Authors: Gallina Clara , Dolgetta Serena , Alloatti Giuseppe , Levi Renzo , Gallo Maria Pia 
Published: October 2011

AbstractIn recent years cardiac tissue engineering has emerged as a promising field aimed at developing suitable techniques to repair the infarcted myocardium with a combination of cells, biomaterials, and regulative factors. In particular it could stand for an alternative strategy to simple in situ cellular implantation. In the present study our purpose was to analyze the interaction between a hyaluronan‐based mesh (HYALONECT®) and neonatal murine ventricular myocytes (NMVMs). Specifically, we investigated morphological and functional characteristics of cardiomyocytes cultured on HYALONECT® in view of its employment in heart repair. Both living and fixed cells analysis was performed on in toto scaffolds with confocal microscopy. NMVMs adhesion on HYALONECT® was studied by tracking sarcomeric α‐actinin immunofluorescence staining. The structural features of NMVMs adherent onto HYALONECT® were investigated at 24, 48, 72 h, and 7 days of culture by immunofluorescence for sarcomeric α‐actinin and connexin‐43. We observed a progressive morphological organization of the cells inside the biopolymer, with both clear sarcomeric arrangement along the scaffold fibers and gap junctions development between adjacent cells. Finally, in vivo intracellular calcium measurements performed using calcium fluorimetric confocal imaging revealed the presence of spontaneous calcium transients and contractile activity of NMVMs adherent onto HYALONECT® up to 48 h from seeding, indicating a progressive differentiation of the cells toward the adult phenotype. In conclusion, our results demonstrate that HYALONECT® allowed NMVMs to adhere to the fibers and to develop functional properties, displaying suitable features as a scaffold to perform heart tissue engineering. J. Cell. Biochem. 113: 800–807, 2012. © 2011 Wiley Periodicals, Inc.
Identification and functional analysis of Zranb2 as a novel Smad‐binding protein that suppresses BMP signaling
Authors: Ohte Satoshi , Kokabu Shoichiro , Iemura Shun‐ichiro , Sasanuma Hiroki , Yoneyama Katsumi , Shin Masashi , Suzuki Seiya , Fukuda Toru , Nakamura Yukio , Jimi Eijiro , Natsume Toru , Katagiri Takenobu 
Published: October 2011

AbstractSmads 1/5/8 transduce the major intracellular signaling of bone morphogenetic proteins (BMPs). In the present study, we analyzed Smad1‐binding proteins in HEK293T cells using a proteomic technique and identified the protein, zinc‐finger, RAN‐binding domain‐containing protein 2 (ZRANB2). Zranb2 interacted strongly with Smad1, Smad5, and Smad8 and weakly with Smad4. The overexpression of Zranb2 inhibited BMP activities in C2C12 myoblasts in vitro, and the injection of Zranb2 mRNA into zebrafish embryos induced weak dorsalization. Deletion analyses of Zranb2 indicated that the serine/arginine‐rich (SR) domain and the glutamine‐rich domain were required for the inhibition of BMP activity and the interaction with Smad1, respectively. Zranb2 was found to be localized in the nucleus; however, the SR domain‐deleted mutant localized to the cytoplasm. The knockdown of endogenous Zranb2 in C2C12 cells enhanced BMP activity. Zranb2 suppressed Smad transcriptional activity without affecting Smad phosphorylation, nuclear localization, or DNA binding. Taken together, these findings suggested that Zranb2 is a novel BMP suppressor that forms a complex with Smads in the nucleus. J. Cell. Biochem. 113: 808–814, 2012. © 2011 Wiley Periodicals, Inc.
Interferons induce the expression of IFITM1 and IFITM3 and suppress the proliferation of rat neonatal cardiomyocytes
Authors: Lau Samantha Lai‐Yee , Yuen Man‐Leuk , Kou Cecy Ying‐Chuck , Au Ka‐Wing , Zhou Junwei , Tsui Stephen Kwok‐Wing 
Published: October 2011

AbstractCardiovascular diseases have been one of the leading killers among the human population worldwide. During the heart development, cardiomyocytes undergo a transition from hyperplastic to hypertrophic growth with an unclear underlying mechanism. In this study, we aim to investigate how interferons differentially stimulate the interferon‐inducible transmembrane (IFITM) family proteins and further be involved in the process of heart development. The expression levels of three IFITM family members, IFITM1, IFITM2, and IFITM3 were investigated during Sprague–Dawley rat myocardial development and differentiation of H9C2 cardiomyocytes. The effects of interferon‐α, ‐β, and ‐γ on DNA synthesis in H9C2 cells were also characterized. Up‐regulation of IFITM1 and IFITM3 were observed during the heart development of Sprague–Dawley rat and the differentiation of H9C2 cells. Moreover, interferon‐α and ‐β induce the expression of IFITM3 while interferon‐γ up‐regulates IFITM1. Finally, interferon‐α and ‐β were demonstrated to inhibit DNA synthesis during H9C2 cell differentiation. Our results indicated interferons are potentially involved in the differentiation and cell proliferation during heart development. J. Cell. Biochem. 113: 841–847, 2012. © 2011 Wiley Periodicals, Inc.
C‐reactive protein promotes adhesion of monocytes to endothelial cells via NADPH oxidase‐mediated oxidative stress
Authors: Huang Xiuqing , Zhang Jing , Liu Jin , Sun Liang , Zhao Hongye , Lu Yonggang , Wang Jianchang , Li Jian 
Published: October 2011

AbstractEnhanced monocyte adhesion to endothelial cells is an early event in atherogenesis. It has been shown that C‐reactive protein (CRP) plays a key role in atherogenesis. Here, we investigated the effects of CRP on monocyte‐endothelial cell adhesion and tested the hypothesis that NADPH oxidase (NOX)‐mediated oxidative stress might play a key role in CRP‐induced monocyte‐endothelial cell adhesion. Firstly, 36 patients with carotid intima‐media thickness (IMT) incrassation and 34 controls were enrolled in this study. The levels of glucose, lipids, CRP, monocyte chemotractant protein (MCP‐1), malondialdehyde (MDA), and protein carbonylation were analyzed. The results showed that carotid IMT was associated with abnormal lipid metabolism, including elevated CRP, triglycerides (TG) (P < 0.01) and decreased high density lipoprotein (HDL) level (P < 0.05). The levels of CRP and MCP‐1 in patients with carotid IMT incrassation were increased compared with the controls (P < 0.01). Moreover, patients with carotid IMT incrassation displayed enhanced MDA and protein carbonylation levels (P < 0.01), accompanied by activation and up‐regulation of NOX in monocytes (P < 0.05) compared with the controls. The monocytes isolated from five healthy donors were used for in vitro experiments. Reactive oxygen species (ROS) production and NOX expression in monocytes were examined. The results also indicated that CRP could promote the adhesion of monocyte‐endothelial cell by up‐regulation of MCP‐1 expression (P < 0.05). Importantly, NFκ B and p38 MAPK signaling pathways, which were activated by NOX‐derived ROS, were involved in CRP‐induced monocyte‐endothelial cell adhesion and up‐regulation of MCP‐1 expression. These data suggested that CRP could promote the adhesion of monocytes to endothelial cells via NOX‐mediated oxidative stress. J. Cell. Biochem. 113: 857–867, 2012. © 2011 Wiley Periodicals, Inc.
Synthesis and characterization of bromo‐, arylazo‐, and heterocyclic‐fused troponoids containing a 1,3‐benzodioxole system
Authors: Li Yang , Sun Mingchun , Gao Wentao 
Published: October 2011

AbstractA facile synthesis of a series of novel bromo‐, arylazo‐, and heterocyclic fused troponoid compounds containing 1,3‐benzodioxole system is described. The 7‐bromo‐, 5,7‐dibromo‐, and 5‐arylazo‐substituted 3‐[(2E)‐3‐(1,3‐benzodioxol‐5‐yl)prop‐2‐enoyl]tropolones (2, 3, and 5, 6, 7) were obtained by direct bromination or azo‐coupling reactions of 3‐[(2E)‐3‐(1,3‐benzodioxol‐5‐yl)prop‐ 2‐enoyl]tropolone (1) with bromine, and diazonium salts of aniline derivatives, respectively. 3‐[(2E)‐3‐(1,3‐Benzodioxol‐5‐yl)prop‐2‐enoyl]‐5‐bromotropolone (4) was obtained from 3‐acetyl‐5‐bromotropolone via one‐pot aldol dehydration reaction with piperonal. Tropolones 2, 3, and 4 were subjected to nucleophilic cyclization with bifunctional hydroxylamine hydrochloride and phenylhydrazine hydrochloride to give the corresponding isoxazolo‐ and pyrazolo‐fused tropones (8, 9, 10, 11, 12, 13), respectively. J. Heterocyclic Chem., (2012).
Heterocyclization of acylthiosemicarbazides
Authors: Hassan Alaa A. , El‐Sheref Essmat M. , Abou‐Zied Ashraf H. 
Published: October 2011

AbstractThe review summarizes published data on the behavior and reactions of acylthiosemicarbazides and their derivatives, which lead to the formation of heterocyclic systems, including methods of preparation in addition to synthesis of pyrrole, thiazole, thiadiazole, thiadiazolidine, and triazole derivatives as well as fused heterocyclic compounds. J. Heterocyclic Chem., (2012).
Radiological findings of primary localized amyloidosis of the ureter
Authors: Tsujioka Yuko , Jinzaki Masahiro , Tanimoto Akihiro , Nakagawa Ken , Akita Hirotaka , Kikuchi Eiji , Okuda Shigeo , Mikami Shuji , Oya Mototsugu , Kuribayashi Sachio 
Published: October 2011

AbstractLocalized amyloidosis is a rare condition, especially that involving the ureter. Because of its rarity and the difficulty in differentiating this condition from urothelial carcinoma by intravenous urography and computed tomography, nephroureterectomy has often been performed unnecessarily for this disease. The authors encountered two cases of this disease, both of which showed a negative urine cytology, no obvious mass effect, and a hypointensity on T2‐weighted imaging. Because these findings are very rare in urothelial carcinoma, ureteroscopy‐guided biopsy was performed, which yielded the diagnosis of amyloidosis. The patients were then treated and followed up at our institute. Primary localized amyloidosis of the ureter should be considered when evaluating ureteric lesions visualized as hypointensities on T2‐weighted images that do not show an obvious mass effect, which could help in the avoidance of unnecessary surgery. J. Magn. Reson. Imaging 2012;431‐435. © 2011 Wiley Periodicals, Inc.
Economic growth and innovation: Lessons in knowledge sharing from bioscience clusters in Ohio and Puerto Rico
Authors: Montalvo Francisco 
Published: October 2011

AbstractAlthough regional industrial clusters have been widely researched, our understanding of knowledge creation and dissemination as a driving force for cluster competitiveness remains unclear. This article examines the nature, utilization, evolution, and effects of knowledge sharing in regional clusters by comparing two industrial regional bioscience clusters at different stages of development: first, by focusing on the everyday experiences of each cluster's local agents, and second, by empirically investigating the impact of specialized knowledge on firm performance in each cluster. Contrary to most of the existing research, foreign direct investment (FDI) by multinational companies (MNCs) can affect the development of local entrepreneurial ecosystems, and spatial structure is not a significant factor in the knowledge creation and dissemination process in clusters. Firm‐level knowledge absorption capabilities, social networks, and knowledge spillover mechanisms, in contrast, correlate significantly with an increase in the creation of specialized knowledge in clusters. © 2011 Wiley Periodicals, Inc.