bluuebunny/crossref_metadata_2025_split huggingface dataset:
PCP auto count: a novel Fiji/ImageJ plug-in for automated quantification of planar cell polarity and cell counting
Authors: Stansak Kendra L. , Baum Luke D. , Ghosh Sumana , Thapa Punam , Vanga Vineel , Walters Bradley J. 
Published: May 2024

Introdution: During development, planes of cells give rise to complex tissues and organs. The proper functioning of these tissues is critically dependent on proper inter- and intra-cellular spatial orientation, a feature known as planar cell polarity (PCP). To study the genetic and environmental factors affecting planar cell polarity, investigators must often manually measure cell orientations, which is a time-consuming endeavor. To automate cell counting and planar cell polarity data collection we developed a Fiji/ImageJ plug-in called PCP Auto Count (PCPA).Methods: PCPA analyzes binary images and identifies “chunks” of white pixels that contain “caves” of infiltrated black pixels. For validation, inner ear sensory epithelia including cochleae and utricles from mice were immunostained for βII-spectrin and imaged with a confocal microscope. Images were preprocessed using existing Fiji functionality to enhance contrast, make binary, and reduce noise. An investigator rated PCPA cochlear hair cell angle measurements for accuracy using a one to five agreement scale. For utricle samples, PCPA derived measurements were directly compared against manually derived angle measurements and the concordance correlation coefficient (CCC) and Bland-Altman limits of agreement were calculated. PCPA was also tested against previously published images examining PCP in various tissues and across various species suggesting fairly broad utility.Results: PCPA was able to recognize and count 99.81% of cochlear hair cells, and was able to obtain ideally accurate planar cell polarity measurements for at least 96% of hair cells. When allowing for a &lt;10° deviation from “perfect” measurements, PCPA’s accuracy increased to 98%–100% for all users and across all samples. When PCPA’s measurements were compared with manual angle measurements for E17.5 utricles there was negligible bias (&lt;0.5°), and a CCC of 0.999. Qualitative examination of example images of Drosophila ommatidia, mouse ependymal cells, and mouse radial progenitors revealed a high level of accuracy for PCPA across a variety of stains, tissue types, and species.Discussion: Altogether, the data suggest that the PCPA plug-in suite is a robust and accurate tool for the automated collection of cell counts and PCP angle measurements.
Characteristics and prognostic value of cardiac magnetic resonance strain analysis in patients with different phenotypes of heart failure
Authors: Zhao Bianjie , Zhang Shiwen , Chen Liang , Xu Kai , Hou Yinglong , Han Shuguang 
Published: May 2024

BackgroundStrain analysis of cardiac magnetic resonance imaging (CMR) is important for the prognosis of heart failure (HF). Herein, we aimed to identify the characteristics and prognostic value of strain analysis revealed by CMR in different HF phenotypes.MethodsParticipants with HF, including HF with reduced ejection fraction, HF with mildly reduced ejection fraction, and HF with preserved ejection fraction, and controls were enrolled. The baseline information and clinical parameters of participants were collected, and echocardiography and CMR examination were performed. Three-dimensional strain analysis was performed in the left ventricle, right ventricle, left atrium, and right atrium using CMR. A multifactor Cox risk proportional model was established to assess the influencing factors of cardiovascular adverse events in patients with HF.ResultsDuring a median follow-up of 999 days (range: 616–1334), 20.6% of participants (73/354) experienced adverse events (HF readmission and/or cardiovascular death). Univariable Cox regression revealed that a 1% increase in left atrial global longitudinal strain (LAGLS) was associated with a hazard ratio (HR) of 1.21 [95% confidence interval (CI):1.15–1.28; P &lt; 0.001]. Left ventricular global circumferential strain (LVGCS) (HR, 1.18; 95% CI: 1.12–1.24; P &lt; 0.001), and left ventricular global longitudinal strain (LVGLS) (HR, 1.27; 95% CI: 1.20–1.36; P &lt; 0.001) were also associated with HF hospitalizations and cardiovascular deaths. Among clinical variables, hypertension (HR, 2.11; 95% CI: 1.33–13.36; P = 0.002), cardiomyopathy (HR, 2.26; 95% CI: 1.42–3.60; P &lt; 0.001) were associated with outcomes in univariable analysis. Multivariable analyses revealed that LAGLS (95% CI: 1.08–1.29; P &lt; 0.001), LVGLS (95% CI:1.08–1.29; P &lt; 0.001) and LVGCS (95% CI: 1.19–1.51; P &lt; 0.001) were significantly associated with outcomes. Among clinical variables, hypertension (95% CI: 1.09–3.73; P &lt; 0.025) remained a risk factor.ConclusionCMR plays an obvious role in phenotyping HF. Strain analysis, particularly left atrial and left ventricular strain analysis (LAGLS, LVGLS, and LVGCS) has good value in predicting adverse outcome events.
Genetic tests as the strongest motivator of cooperation between participants and biobanks–Findings from cross-sectional study
Authors: Majchrowska Anita , Pawlikowski Jakub , Sak Jarosław , Świerczyńska Blanka , Suchodolska Magdalena 
Published: May 2024

IntroductionThe development of the scientific potential linked with biobanking and research on human biological material is highly dependent on the willingness of potential donors to cooperate with entities that collect the material. For this reason, it is crucial to identify the circumstances and factors that may encourage potential participants to donate their biological material. In particular, knowledge of the motivational factors that can be modified by the persons managing a biobank may prove notably important for shaping the organizational and communication policy of the biobank and other scientific institutions.Material and methodsThe research was carried out on a group of 1,100 people over 18 years of age representing the adult population of Poland in 2021.ResultsMore than half of the respondents declared their willingness to donate a blood sample for research purposes to a biobank (57.8%). The most often indicated incentives among the factors supporting the donation of biological material were offers of: obtaining the results of genetic tests predicting the risk of diseases (77.1%), blood tests (71.3%), the possibility of obtaining a small remuneration (64.6%) and the carrying out of genetic ancestry tests (60.4%).ConclusionOffering the possibility of performing additional diagnostic tests, especially genetic tests, may significantly increase the willingness of potential donors to cooperate with biobanks and other entities collecting human biological material for the purpose of scientific research. However, attention should also be paid to the challenges and risks linked with respecting the privacy and autonomy of research participants.
Recognition of facial emotion based on SOAR model
Authors: Ramzani Shahrestani Matin , Motamed Sara , Yamaghani Mohammadreza 
Published: May 2024

IntroductionExpressing emotions play a special role in daily communication, and one of the most essential methods in detecting emotions is to detect facial emotional states. Therefore, one of the crucial aspects of the natural human–machine interaction is the recognition of facial expressions and the creation of feedback, according to the perceived emotion.MethodsTo implement each part of this model, two main steps have been introduced. The first step is reading the video and converting it to images and preprocessing on them. The next step is to use the combination of 3D convolutional neural network (3DCNN) and learning automata (LA) to classify and detect the rate of facial emotional recognition. The reason for choosing 3DCNN in our model is that no dimension is removed from the images, and considering the temporal information in dynamic images leads to more efficient and better classification. In addition, the training of the 3DCNN network in calculating the backpropagation error is adjusted by LA so that both the efficiency of the proposed model is increased, and the working memory part of the SOAR model can be implemented.Results and discussionDue to the importance of the topic, this article presents an efficient method for recognizing emotional states from facial images based on a mixed deep learning and cognitive model called SOAR. Among the objectives of the proposed model, it is possible to mention providing a model for learning the time order of frames in the movie and providing a model for better display of visual features, increasing the recognition rate. The accuracy of recognition rate of facial emotional states in the proposed model is 85.3%. To compare the effectiveness of the proposed model with other models, this model has been compared with competing models. By examining the results, we found that the proposed model has a better performance than other models.
The epidemiology and associated factors of non-exclusive breastfeeding: a comparative cross-sectional study of livelihood-secure and insecure areas
Authors: Negesse Ayenew , Girma Tsinuel , Desalegn Beruk Berhanu , Kerac Marko , Berhane Melkamu 
Published: May 2024

IntroductionRegardless of national commitment, non-exclusive breastfeeding (NEBF) is a public health problem that worsens over time. It can be associated with sociodemographic, economic, and environmental factors and may vary depending on livelihood security. Hence, this study aimed to determine the magnitude of NEBF and identify its associated factors by considering two areas with varied degrees of livelihood security.MethodsThis study represented a comparative cross-sectional survey of 1,060 under 6 months (u6m) infant–mother pairs. Both descriptive and analytic statistics were evaluated using STATA version 17 packages. A binary logistic regression was used to identify associated factors of NEBF. The odds ratio (OR) with a 95% confidence interval (CI) was used to measure the significance of the association at a p-value of &lt;0.05.ResultsThe pooled magnitude of 51% of NEBF mothers (95% CI: 48.0, 54.0) was 53.1% (95% CI: 49.2, 57.0) and 48.1% (95% CI: 43.4, 52.8) in livelihood-secure and livelihood-insecure areas, respectively. The lack of recollecting the infant’s birth date by mothers (AOR = 2.4; 95% CI = 1.15–4.40) had the highest odds of NEBF while household heads with tertiary education (AOR = 0.14; 95% CI = 0.01–0.54) and the poorest households (AOR = 0.43; 95%CI = 0.20–0.82) had the lowest odds of NEBF in livelihood-secure areas but not in livelihood-insecure areas. Moreover, mothers with male infants (AOR = 1.9; 95% CI = 1.18–2.92) had high odds of NEBF in livelihood-insecure areas but not in livelihood-secure areas. Infants of 2 to less than 4-month-old (AOR = 8.5; 95% CI = 3.47–18.63) and 4 to less than 6-month-old (AOR = 22.2; 95% CI = 8.02–51.97) in livelihood-secure areas and infants of 2 to less than 4-month-old (AOR = 4.3; 95% CI = 1.29–11.67) and 4 to less than 6-month-old (AOR = 8.3; 95% CI = 2.44–22.39) in livelihood-insecure areas had high odds of NEBF.ConclusionOver half of the mothers were practicing NEBF, which represents a failure to meet national and international targets. Area vulnerability to livelihood security modifies factors of NEBF. Male infants in insecure areas, infants of unknown age in secure areas, and infants aged 2 months or older, regardless of setting, were more vulnerable to NEBF. However, households with the lowest wealth and higher household head educational status in livelihood-secure areas were less vulnerable to NEBF. Hence, livelihood-based interventions targeting mothers of 2 to less than 6-month-old infants, with emphasis on these factors, may help address and reduce NEBF.
Mendelian randomization and single-cell expression analyses identify the causal relationship between depression and chronic rhinosinusitis
Authors: Zhou Fangwei , Yang Yan , Li Jianyao , Jin Ying , Zhang Tian , Yu Guodong 
Published: May 2024

BackgroundThe causative relationship between chronic rhinosinusitis (CRS) and depression remains unclear. Herein we employed Mendelian randomization (MR) coupled with single-cell analysis to investigate the causality between CRS and depression.MethodsData pertaining to CRS and depression were mined from the genome-wide association study database, and a single-cell dataset was sourced from the literature. To explore causality, we conducted bidirectional MR analysis using MR-Egger, weighted median, inverse variance weighted (IVW), simple mode, and weighted mode, with IVW representing the most important method. Further, sensitivity analysis was performed to evaluate the robustness of MR analysis results. Candidate genes were analyzed via single-cell combined MR analysis.ResultsForward MR analysis indicated depression as a risk factor for CRS when depression was the exposure factor and CRS was the outcome (OR = 1.425, P &lt; 0.001). Reverse MR analysis revealed the same positive relationship between CRS and depression when CRS was the exposure factor and depression was the outcome (OR = 1.012, P = 0.038). Sensitivity analysis validated the robustness of bidirectional MR analysis results. Ten cell types (endothelial, ciliated, basal, myeloid, mast, apical, plasma, glandular, fibroblast, and T cells) were identified in the single-cell dataset. The network of receptor–ligand pairs showed that in normal samples, cell–cell interactions were present among various cell types, such as epithelial, mast, myeloid, and endothelial cells. In contrast, CRS samples featured only one specific receptor–ligand pair, confined to myeloid cells. TCF4 and MEF2C emerged as potentially crucial for CRS-associated depression development.ConclusionsOur findings suggest a bidirectional causal relationship between CRS and depression, offering a new perspective on the association between CRS and depression.
The worldwide trend in diabetes awareness, treatment, and control from 1985 to 2022: a systematic review and meta-analysis of 233 population-representative studies
Authors: Shahrestanaki Ehsan , Mohammadian Khonsari Nami , Seif Ehsan , Baygi Fereshteh , Ejtahed Hanieh-Sadat , Sheidaei Ali , Djalalinia Shirin , Magliano Dianna J. , Qorbani Mostafa 
Published: May 2024

BackgroundWith the rapid increase in the prevalence of DM, studies on the awareness, treatment, and control of this condition are essential. Therefore, this study aimed to review the literature and pool the awareness, treatment, and control of diabetes at the global, regional, and national levels.MethodsIn this systematic review and meta-analysis, several databases, including MEDLINE/PubMed, Institute of Scientific Information (ISI), Scopus, and Google Scholar, were searched using appropriate keywords up to June 2022. Observational studies investigating the awareness, treatment, and control of glucose levels among diabetic individuals were included. Awareness, treatment, and control were defined as the proportion of participants who were aware of their diabetes condition, treated pharmacologically, and achieved adequate glucose control, respectively. Two investigators independently conducted the study selection, data extraction, and quality assessment. Heterogeneity among studies was calculated using Chi-square, and a random-effect meta-analysis was used to pool the rates.ResultsA total of 233 studies published between 1985 and 2022 met the inclusion criteria. The included studies had a combined population of 12,537,968. The pooled awareness of DM was 60% (95%CI: 56–63) and ranged from 41% (25–57) in low-income countries to 68% (64–72) in high-income countries, with no significant trend observed over the assessed periods at the global level. The pooled treatment of DM globally was 45% (42–48) and varied from 37% (31–43) in lower-middle-income countries to 53% (47–59) in high-income countries, showing variation over the examined time period. Before 2000, the proportion of adequate DM control was 16% (12–20), which significantly improved and reached 22% (19–25) after 2010. The pooled awareness, treatment, and control of DM were higher in females, high-income countries, and urban areas compared to males, upper and lower-middle-income countries, and rural areas, respectively. The older adults population had higher awareness and treatment rates than the adult population, but their DM control did not differ significantly.ConclusionDespite the high level of awareness and treatment among the diabetic population, treatment success (control) is considerably low, particularly in low-income countries and rural areas. It is crucial to improve awareness, treatment, and control by strengthening the primary care system in all countries.
Raman Spectroscopy on Free-Base Meso-tetra(4-pyridyl) Porphyrin under Conditions of Low Temperature and High Hydrostatic Pressure
Authors: dos Reis Jhon Rewllyson Torres , Leite Fabio Furtado , Sharma Keshav , Ribeiro Guilherme Almeida Silva , Silva Welesson Henrique Natanael , Batista Alzir Azevedo , Paschoal Alexandre Rocha , Paraguassu Waldeci , Mazzoni Mario , Barbosa Neto Newton Martins , Araujo Paulo Trindade 
Published: May 2024

We present a Raman spectroscopy study of the vibrational properties of free-base meso-tetra(4-pyridyl) porphyrin polycrystals under various temperature and hydrostatic pressure conditions. The combination of experimental results and Density Functional Theory (DFT) calculations allows us to assign most of the observed Raman bands. The modifications in the Raman spectra when excited with 488 nm and 532 nm laser lights indicate that a resonance effect in the Qy band is taking place. The pressure-dependent results show that the resonance conditions change with increasing pressure, probably due to the shift of the electronic transitions. The temperature-dependent results show that the relative intensities of the Raman modes change at low temperatures, while no frequency shifts are observed. The experimental and theoretical analysis presented here suggest that these molecules are well represented by the C2v point symmetry group.
Enhanced field-effect mobility (>250 cm2/V·s) in GaN MOSFETs with deposited gate oxides via mist CVD
Authors: Ikeyama Kazuki , Tomita Hidemoto , Harada Sayaka , Okawa Takashi , Liu Li , Kawaharamura Toshiyuki , Miyake Hiroki , Nagasato Yoshitaka 
Published: May 2024

Abstract
We report an enhanced field-effect mobility (>250 cm2·V−1·s−1) in GaN MOSFETs. High mobility was achieved by reducing the oxidation of the GaN surface, which was a major factor affecting channel mobility in GaN MOSFETs. Among various gate oxide deposition methods, mist CVD using O3 suppressed GaN surface oxidation. The best field-effect mobility was observed using mist CVD-deposited gate oxides, achieving a peak mobility of 266 cm2·V−1·s−1 with a high threshold voltage of 4.8 V.
Kryviy Rih as the center of heavy industry of the USSR: the role of Kryvbas scientists in the development of the mining industry
Authors: Doіar Larysa 
Published: May 2024

The article completes the cycle of investigations within the framework of the historical and foundational research of the State Printing Archive "Printed editions of the 1970s: thematic and content priorities", planned for 2023. The object of the research was the priority topics that were covered in domestic printed editions of the specified period , the subject of which is the periodical and continuing editions published in Kryvyi Rih during the 1970s, namely: the yearbook "Mining ore production", published by the Scientific and Research Mining and Ore Institute (SRMOI) of Kryvyi Rih, and materials of all-Union and republican scientific and technical forums (seminars, conferences, etc.). Relying on the specified primary source base, the author considered the main directions of development of mining science in Kryvyi Rih during the researched period. The purpose of the proposed exploration is to form conclusive generalizations about today&#39;s little-known local prints, conduct a content analysis of their content, update important historical events from the past of Kryvyi Rih and personify the mining science of the Kryvyi Rih iron ore basin. Having achieved the set goals, the author claims that in the 1970s, Kryvyi Rih became an all-Union recognized center of scientific and practical mining. In the city, despite its modest (district) administrative-territorial status, powerful scientific schools were formed, higher, secondary special and vocational-technical mining education flourished. Having a closed cycle: from mining (Kryvyi Rih mines, quarries) and beneficiation of iron ore (five mining and beneficiation plants operate on the territory of the city) to the production of the best types of iron and steel (Metallurgical Plant "Kryvorizhstal", Kryvyi Rih Coke Chemical Plant) — Kryvyi Rih became the industrial pearl of the Soviet Union. In modern Ukraine, the city has rightly earned the name "iron heart of the state".
The Ukrainian question in the historical and cultural heritage of the patriot writer I. S. Nechuy-Levytskyi: observations of sources
Authors: Shumslova Anzhela 
Published: May 2024

The article focuses on the role of Ivan Semenovych Nechuy-Levytskyi in the historical and cultural heritage of Ukraine. The variety of topics and literary trends that the writer's work synthesized in itself still attracts scientists today. Ways to heal our society, unite it, and return to the European community lie in turning to the national primary sources in establishing our national statehood, in restoring the prestige of national values. The purpose of the study is a source-based analysis of the work of the outstanding writer-patriot Ivan Semenovych Nechuy-Levytskyi as an information resource for further scientific research into the relationship between the Ukrainian question and the political situation in the country, the development of culture and education. The research methodology is based on general scientific methods (analysis, synthesis, comparison, descriptive, detailing, generalization) and special-historical (historical-source analysis, biographical). The scientific novelty consists in the presentation of information about the multi-vector direction of the writer's creativity. The relevance of the research is determined by the search for new assessments or classifications for rethinking intellectual experience and a new understanding of the role and significance of the figure of Ivan Semenovych Nechuy-Levytskyi in the Ukrainian cultural consciousness. Conclusions. The theoretical aspect of the problem of research and development of the collection of I. Nechuy-Levytskyi's publications is summarized. The themes of the artist's works and publications are outlined. The information contained in the writer's writings significantly expands knowledge about his activities, illuminates cooperation with domestic and foreign artists, scientists and specifies his contribution to the development of culture, education, reproduction of an objective picture of historical events in Ukraine. The analysis and scientific study of the journalistic and literary and artistic heritage of I. Nechuy-Levytskyi, which is part of the funds of the National Library of Ukraine named after V. I. Vernadskyi, shows that this collection is of great interest to researchers of biography, literary studies, linguistics and history of Ukraine. Balanced, thoughtful use of the works of Ivan Semenovych Nechuy-Levytskyi will contribute to the development of further research into the life and work of the outstanding Ukrainian writer, effective preservation of mental stability, and productive information activities of citizens in the difficult conditions of the current challenges facing the Ukrainian people.