bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of low molecular weight plasma proteins using ultrafiltration and large gel two‐dimensional electrophoresis
Authors: Jung Woon‐Won , Phark Sohee , Oh Sangnam , Khim Jin‐Young , Lee Juneyoung , Nam Myung Hee , Seo Jong Bok , Park So‐Young , Jo Eunkyung , Choi Seonyoung , Zheng Zhi , Lee Ji Young , Lee Min , Lee Eunil , Sul Donggeun 
Published: March 2009

AbstractIn this study, various solvent systems were applied to obtain a high and consistent recovery rate of low molecular weight plasma proteins (LMPP) from human plasma. A buffer system containing 7 M urea, 2 M thiourea, 25 mM NH4HCO3 + 20% ACN (pH 8.2) produced the highest recovery rate of LMPP. To validate the recovery of cut off membrane (COM) obtained using the urea buffer system, 27 different 30 kDa COMs were used to prepare the LMPP sample which were then subjected to 1‐D SDS‐PAGE. Statistical analysis showed that the buffer system with COM produced a consistent the recovery of LMPP. In addition, 2‐DE analysis was also conducted to determine the relative intensity of each protein spot. When molecular weight ranges over 30 kDa and under 30 kDa were evaluated, 953 and 587 protein spots were observed in the gels, respectively, resulting in a total of 1540 protein spots being resolved. Identification of the major proteins were then performed using a nano‐LC/MS system comprised of an HPLC system and an ESI‐quadrupole IT MS equipped with a nano‐ESI source.
Subproteome analysis of the neutrophil cytoskeleton
Authors: Xu Ping , Crawford Mark , Way Michael , Godovac‐Zimmermann Jasminka , Segal Anthony W. , Radulovic Marko 
Published: March 2009

AbstractNeutrophils play a key role in the early host‐defense mechanisms due to their capacity to migrate into inflamed tissues and phagocytose microorganisms. The cytoskeleton has an essential role in these neutrophil functions, however, its composition is still poorly understood. We separately analyzed different cytoskeletal compartments: cytosolic skeleton, phagosome membrane skeleton, and plasma membrane skeleton. Using a proteomic approach, 138 nonredundant proteins were identified. Proteins not previously known to associate with the skeleton were: n‐acetylglucosamine kinase, phosphoglycerate mutase 1, prohibitin, ficolin‐1, phosphogluconate dehydrogenase, glucosidase, transketolase, major vault protein, valosin‐containing protein, aldehyde dehydrogenase, and lung cancer‐related protein‐8 (LCRP8). The majority of these proteins can be classified as energy metabolism enzymes. Such a finding was interesting because neutrophil energy metabolism is unusual, mainly relying on glycolysis. The enrichment of phosphoglycerate mutase in cytosolic skeleton was additionally indicated by the use of Western blotting. This is the broadest subcellular investigation to date of the neutrophil cytoskeletal proteome and the first proteomic analysis in any cell type of the phagosome skeleton. The association of metabolic enzymes with cytoskeleton is suggestive of the importance of their localized enrichment and macromolecular organization in neutrophils.
7th HUPO World Congress: The Human Disease Glycomics/Proteomics Initiative (HGPI) Session 17 August 2008, Amsterdam, The Netherlands
Authors: Pierce J. Michael , Taniguchi Naoyuki 
Published: March 2009

AbstractThe Human Disease Glycomics/Proteomics Initiative (HGPI) Session was held on August 17, 2008, at the HUPO World Congress in Amsterdam. Reports were made on the progress of the first and second analytical pilot studies to profile N‐ and O‐linked glycan structures of standard glycoproteins utilizing laboratories from around the world. In addition, recent advances in glycan structural analyses were presented, including the use of O‐linked glycan libraries of standards, use of negative mode nano‐LC‐MS for O‐linked glycan analysis, and identification of aberrant O‐glycosylation of IgA1 as a cause of IgA nephropathy. A report was made of a newly discovered lectin, malectin, which appears to function in the folding/quality control of glycoproteins with N‐linked glycans and may regulate several human disorders whose etiology involves protein quality control in the ER. The major glycan ligand for malectin was identified using a novel printed glycan microarray. Advances in the analysis of the genes that are associated with glycan expression and recognition – the glycotranscriptome – were described, as well as technologies to determine the relative quantitation of N‐ and O‐linked glycans from as few as 2×106 cells. These technologies are being applied to identify potential biomarkers of stem and cancer cells.
Polymerization of Halogenophosphazenes in Capacitively Induced Plasma Discharges
Authors: Vorac Zbynek , Alberti Milan , Janca Jan 
Published: March 2009

AbstractThis work studies the products of plasma polymerized (NPCl2)3, (NPCl2)4 and (NPF2)3. Reaction products were examined by the 31P‐NMR, LDI‐TOF MS, DIP‐MS, IR and XPS. To achieve a successful reaction of (NPCl2)4 it is crucial that the phosphazene is carefully heated to a temperature of about 60 °C. Analysis demonstrated that products of plasma polymerization are similar for all halogenophosphazenes and mainly consist of branched chains. The best homogeneity, adhesion to substrate and also easiest substitution of halogen atoms by nucleophilic reagents was achieved with plasma polymerized (NPF2)3. Prepared polymers could be used for surface improvement and as flame‐retardants.magnified image
Configuration of Plasma Processes for the Generation of a Chemical Triple Pattern for Cell‐Based RNA Arrays
Authors: Schröder Karsten , Busse Benedikt , Steffen Hartmut , Ohl Andreas , Quade Antje , Weltmann Klaus‐Dieter 
Published: March 2009

AbstractA threefold chemical patterning of polymer surfaces has been investigated for cell‐based biomedical diagnostics. Therefore, plasma processes have to be configured to realize three different cytocompatible surface modifications without any deterioration of optical properties. Sufficient chemical contrasts of different surface zones were realized by the application of pulsed downstream microwave plasmas, which also allowed the reduction of thermal load to the substrate. Sufficient contrasts in subsequent processing steps were realized with good local definition using polymeric masks in tight contact with the polymeric surface.
NMR Study of Polyethylene‐Like Plasma Polymer Films
Authors: Hanuš Jan , Hanyková Lenka , Choukourov Andrei , Kousal Jaroslav , Polonskyi Oleksandr , Slavínská Danka , Biederman Hynek 
Published: March 2009

AbstractThermal decomposition (“evaporation”) of polyethylene (PE) was performed with or without an activation of volatile fragments in an rf glow discharge. Study of the deposition process via quadrupole mass spectrometry showed the presence of hydrocarbon fragments up to 100 amu. Properties of the resulting films were investigated by means of FTIR, XPS, and NMR spectroscopy. These analyses showed that the activation of polymeric fragments in plasma enhances cross‐linking and the films become hydrocarbon plasma polymers. However, these plasma polymers resemble much more the original PE. In addition, NMR technique enabled to assess the ratio of crystalline and amorphous phases in the films.
SiCx Layers Prepared by Hybrid Laser Deposition and PLD
Authors: Jelínek Miroslav , Kocourek Tomáš , Zemek Josef , Kadlec Jaromír 
Published: March 2009

AbstractThin SiC films were fabricated using hybrid laser‐magnetron deposition system and pulsed laser deposition (PLD). In the hybrid system, a KrF excimer laser was used for deposition of carbon and magnetron at the same time for sputtering of Si species. Films were fabricated in hydrogen/argon, or in argon ambient. Results were compared with SiC films created using PLD only. Substrate temperature (Ts) was changed from room temperature (RT) up to 800 °C. Film topology, crystallinity, composition and chemical bonds were studied. Polycrystalline SiC films were prepared by both deposition techniques, in argon atmosphere, for Ts > 600 °C.
Structural and Mechanical Study of Nanocomposite Ti–Zr–C–H Coatings Prepared by Reactive Magnetron Sputtering
Authors: Martínez‐Martínez Diego , Kubová Olga , Delplancke‐Ogletree Marie‐Paule 
Published: March 2009

AbstractNanocomposite structures formed by small crystallites embedded in a lubricant matrix present moderate–high hardness, good toughness, and low values of friction coefficient and wear rate, and they are a promising alternative for protective tasks. In this study, we have prepared Ti–Zr–C–H films as potential candidates as protective layers for biocompatible applications. The constituting elements were selected due to their proven good biocompatibility at the macroscale. The films were prepared by reactive dc magnetron sputtering, and further characterized by X‐ray photoelectron spectroscopy and X‐ray diffraction. Mechanical and tribological properties were also evaluated.
Thermal Stability and Phase Transformations of γ‐/Amorphous‐Al2O3 Thin Films
Authors: Eklund Per , Sridharan Madanagurusamy , Singh Gurvinder , Bøttiger Jørgen 
Published: March 2009

AbstractMagnetron‐sputtered Al2O3 thin films were annealed in ambient air. The phase compositions of the as‐deposited Al2O3 films were (i) fully amorphous, (ii) nanocrystalline γ‐Al2O3 in an amorphous Al2O3 matrix, and (iii) fully crystalline γ. For all samples, annealing to 1 100–1 150 °C resulted in a transformation to α‐alumina. The transformation paths depend on the phase fraction of γ in the as‐deposited films. For amorphous films and films with low initial γ fraction, the intermediate phase θ‐Al2O3 appeared in the range of 1 000–1 100 °C. For predominantly crystalline γ‐Al2O3 as‐deposited films no intermediate Al2O3 phases were observed, indicating a direct γ‐to‐α phase transformation at ∼1 100 °C.
Industrialisation Study of Nanocomposite nc‐TiC/a‐C Coatings for Electrical Contact Applications
Authors: Lewin Erik , Olsson Erik , André Benny , Joelsson Torbjörn , Öberg Åke , Wiklund Urban , Ljungcrantz Henrik , Jansson Ulf 
Published: March 2009

AbstractNanocomposite nc‐TiC/a‐C coatings were prepared by non‐reactive magnetron sputtering in industrial scale equipment, under varying deposition conditions in order to investigate up‐scaling and possible industrialisation. The coatings were found to have similar microstructure and performance compared to previous laboratory scale experiments. The samples were characterised with XRD, XPS and SEM as well with ball‐on‐disc, nanoindentation and electrical measurements. Coatings containing a small fraction of a‐C matrix phase were found to have promising both electrical properties (ρ < 400 µΩcm and contact resistances down to 0.34 mΩ at 40 N) and tribological properties (f < 0.3 for 10 000 laps).
Method to Determine the Sticking Coefficient of O2 on Deposited Al During Reactive Magnetron Sputtering, Using Mass Spectrometry
Authors: Leroy Wouter P. , Mahieu Stijn , Persoons Rosita , Depla Diederik 
Published: March 2009

AbstractModels describing the reactive sputtering process need experimental input for some important parameters as the knock‐on yield, the reaction rate constant, the sticking coefficient on the target and the sticking coefficient on the deposited material. Here, a method is described to calculate the sticking coefficient of O2 on the deposited material, during reactive magnetron sputtering. Mass spectrometry is used to measure local, effective O2 partial pressure during sputtering, at the position of the sample. Combined with electron probe microanalysis of the oxygen content in deposited layers, we calculate the sticking coefficient. At our conditions, a local, effective pressure of (5.26 ± 0.44) × 10−6 mbar was found and an average sticking coefficient of 0.107 ± 0.032 for O2 on deposited aluminium during reactive magnetron sputtering.
Effect of annealing for CuInS2 thin films prepared from Cu‐rich ternary compound
Authors: Akaki Yoji , Nakamura Shigeuki , Nomoto Keita , Yoshitake Tsuyoshi , Yoshino Kenji 
Published: March 2009

AbstractEvaporated CuInS2 films using a single‐source were annealed in H2S atmosphere from 250 to 500 °C for 60 min. after the evaporation. Polycrystalline CuInS2 powder grown by a hot‐press method was employed as a source material. Cu/In ratios of the source were 1.0, 1.2 and 1.5. All the films annealed above 350 °C were of CuInS2 single phase regardless of the Cu/In ratio of the source material. The films prepared from the source material of the Cu/In ratio of 1.5 became Cu‐rich films, which had the Cu/In ratio of 1.37. Carrier concentrations, resistivities and mobilities of the films annealed above 350 °C prepared from the source material of the Cu/In ratio of 1.5 were approximately 1×1021 cm–3, 0.1 Ωcm and 0.1 cm2/Vs, respectively, at room temperature. The activation energy of the film annealed at 400 °C was evaluated to be 6.5 meV. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Market orientation, marketing capabilities, and firm performance
Authors: Morgan Neil A. , Vorhies Douglas W. , Mason Charlotte H. 
Published: March 2009

AbstractDrawing on traditional resource‐based theory and its recent dynamic capabilities theory extensions, we examine both the possession of a market orientation and the marketing capabilities through which resources are deployed into the marketplace as drivers of firm performance in a cross‐industry sample. Our findings indicate that market orientation and marketing capabilities are complementary assets that contribute to superior firm performance. We also find that market orientation has a direct effect on firms' return on assets (ROA), and that marketing capabilities directly impact both ROA and perceived firm performance. Copyright © 2009 John Wiley & Sons, Ltd.
Phosphidtelluriden auf der Spur: Zum System Ce/Te/P. The Trace to Phosphide Tellurides: The System Ce/Te/P
Authors: Philipp Frauke , Pinkert Katja , Schmidt Peer 
Published: March 2009

AbstractThe cerium phosphide tellurides Ce3Te4–xPx (x ≤ 1), CeTe2–xPx (x ≈ 0.35–0.55) and CeTe2–yPy (y ≥ 1) have been synthesised from the elements or from oxides in a thermite‐type reaction. Both ways have been optimised by consideration of the thermodynamic behaviour of the compounds. Hence the investigation of phase equilibria in the ternary system Ce/Te/P and of the thermal decomposition of ternary phases was necessary. This investigation was performed by using different experimental approaches as total‐pressure measurements and thermal analysis. It was shown that the cerium phosphide tellurides decompose in consecutive steps into CeTe(s) and gaseous phosphorus, [P4(g)]. The results were supported and further analysed by thermodynamic modelling of the ternary system; the computed phase diagrams of the systems Ce/Te and Ce/Te/P are presented.
Structure and Optical Properties of the Arsenide Oxides REZnAsO (RE = Y, La–Nd, Sm, Gd–Er)
Authors: Lincke Hannes , Glaum Robert , Dittrich Volker , Möller Manfred H. , Pöttgen Rainer 
Published: March 2009

AbstractThe equiatomic rare earth metal zinc arsenide oxides REZnAsO (RE = Y, La–Nd, Sm, Gd–Er) were synthesized in well crystallized form from the elements, arsenic, and ZnO in NaCl/KCl fluxes in sealed silica ampoules. The samples were characterized by powder and single‐crystal X‐ray data. HoZnAsO and ErZnAsO are reported for the first time. Four structures (ZrCuSiAs type, space group P4/nmm) were refined from single‐crystal X‐ray diffractometer data: a = 394.23(3), c = 884.0(1) pm, wR2 = 0.0345, 216 F2 values for YZnAsO, a = 409.56(3), c = 907.0(1) pm, wR2 = 0.0502, 285 F2 values for LaZnAsO, a = 397.62(4), c = 889.2(2) pm, wR2 = 0.0700, 216 F2 values for GdZnAsO, and a = 392.72(3), c = 881.5(1) pm, wR2 = 0.0580, 373 F2 values for ErZnAsO with 12 variables per refinement. The REZnAsO structures consist of alternate stacks of (RE3+O2–) and (Zn2+As3–) layers with covalent RE–O (224 pm in ErZnAsO) and Zn–As (255 pm in ErZnAsO) intralayer and weak ionic interlayer bonding. Both layers consist of condensed edge‐sharing OEr4/4 and ZnAs4/4 tetrahedra. In agreement with an electron precise formulation RE3+Zn2+As3–O2–, the crystals are transparent with yellow (LaZnAsO) to red color tones. In agreement with the different colors, LaZnAsO shows a significantly higher optical bandgap of Eg ≈ 2.3 eV than arsenide oxides REZnAsO (RE = Y, Pr, Nd, Sm) (Eg ≈ 1.9 eV).
High Temperature Phase and Thermal Expansion of PrBr3
Authors: Krämer Karl W. , Keller Lukas 
Published: March 2009

AbstractThe thermal expansion and phase transition of PrBr3 was investigated by neutron diffraction and DSC measurements. PrBr3 has a low‐temperature (LT) UCl3‐type modification and a high‐temperature (HT) PuBr3‐type modification. The stability range and structure of the HT phase were established for the first time by diffraction experiments. The thermal expansion of the LT modification was investigated by powder neutron diffraction from 1.6 K to 940 K. It shows a very anisotropic expansion of 3.5 % along the a‐axis up to 940 K, but along the c‐axis a small expansion of 0.14 % up to 445 K is followed by a contraction about 0.4 % up to 940 K. On heating, the LT phase is observed up to the melting point at 962 K. A partial transformation to the HT phase takes place above 956 K. On cooling, the HT phase crystallises from the melt at 946 K and transforms to the LT phase at 930 K.
Synthesis and Characterization of Mononuclear and Dinuclear Manganese Bis‐acetylide Complexes
Authors: Fritz Thorsten , Schmalle Helmut W. , Blacque Olivier , Venkatesan Koushik , Berke Heinz 
Published: March 2009

AbstractThe symmetric d5 trans‐bis‐alkynyl complexes {Mn[(Me)2P(CH2)nP(Me)2]2(C≡CC6H4R)2} (n = 2, R = ‐C≡CTIPS (2a); n = 3, R = ‐C≡CTIPS (2b); n = 2, R = F (4); n = 3; ‐C≡CTIPS) were prepared by the reaction of [Mn(dmpe)2Br2] with two equivalents of the corresponding acetylide LiC≡CC6H4R (R = F, ‐C≡CTIPS). The reaction of compounds 2a, 2b and 4 with [Cp2Fe][PF6] yielded the corresponding d4 complexes {Mn[(Me)2P(CH2)nP(Me)2]2(C≡CC6H4R)2}[PF6] (n = 2, R = ‐C≡CTIPS, [2a]+; n = 3, R = ‐C≡CTIPS, [2b]+;n = 2, R = F, [4]+; n = 3; ‐C≡CTIPS). The unsymmetrically substituted trans‐iodo‐alkynyl complex [IMn(dmpe)2(C≡CC6H4‐C≡CTIPS)] (5) was obtained by treating MeCpMn(dmpe)I with one equivalent of H‐C≡CC6H4‐C≡CTIPS in the presenceof dmpe. Deprotection of the TIPS group with TBAF gavecomplex [IMn(dmpe)2(C≡CC6H4C≡CH)] (6) in 94 % yield. The neutral dinuclear complexes MnII/MnII compounds {[Mn((Me)2P(CH2CH2)P(Me)2)2(C≡CC6H4R)]2(μ‐C4)} (R = H, Me, n‐pentyl, F) were prepared by the direct reaction of {[Mn((Me)2P(CH2CH2)P(Me)2)2I]2(μ‐C4)} with the corresponding LiC≡CC6H4R (R = H, n‐pentyl, F). These MnII/MnII dinuclear compounds were further oxidized to the mixed‐valent complexes and the dicationic complexes using [Cp2Fe][PF6]. All complexes have been characterized by NMR, IR, and Raman spectroscopy. X‐ray diffraction studies were carried out on complexes 1c, 2a, 4, [4]+, and 6.
A formal framework for modeling and validating Simulink diagrams
Authors: Chen Chunqing , Dong Jin Song , Sun Jun 
Published: March 2009

AbstractSimulink has been widely used in industry to model and simulate embedded systems. With the increasing usage of embedded systems in real-time safety-critical situations, Simulink becomes deficient to analyze (timing) requirements with high-level assurance. In this article, we apply Timed Interval Calculus (TIC), a real-time specification language, to complement Simulink with TIC formal verification capability. We elaborately construct TIC library functions to model Simulink library blocks which are used to compose Simulink diagrams. Next, Simulink diagrams are automatically transformed into TIC models which preserve functional and timing aspects. Important requirements such as timing bounded liveness can be precisely specified in TIC for whole diagrams or some components. Lastly, validation of TIC models can be rigorously conducted with a high degree of automation using a generic theorem prover. Our framework can enlarge the design space by representing environment properties to open systems, and handle complex diagrams as the analysis of continuous and discrete behavior is supported.
Thymic Neuroendocrine Tumors in Multiple Endocrine Neoplasia Type 1: A Comparative Study on 21 Cases Among a Series of 761 MEN1 from the GTE (Groupe des Tumeurs Endocrines)
Authors: Goudet Pierre , Murat Arnaud , Cardot‐Bauters Catherine , Emy Philippe , Baudin Eric , du Boullay Choplin Hélène , Chapuis Yves , Kraimps Jean‐Louis , Sadoul Jean‐Louis , Tabarin Antoine , Vergès Bruno , Carnaille Bruno , Niccoli‐Sire Patricia , Costa Annie , Calender Alain ,   The members of the GTE network (Groupe des Tumeurs Endocrines)
Published: March 2009

AbstractBackgroundThymic neuroendocrine tumors (Th‐NET) present a poor prognosis for patients with multiple endocrine neoplasia type 1 (MEN1). The purpose of this article was to study the clinical, biological, and pathological features of Th‐NET in a large cohort of patients with MEN1.MethodsThe 761‐patient MEN1 cohort from the GTE registry was used (Groupe des Tumeurs Endocrines).ResultsThe actuarial probability of occurrence was 2.6% (range, 1.3–5.5%) at aged 40 years. All, except one, Th‐NET patients were men. Four patients had no other associated lesions. The youngest patient was aged 16 years. Mean age at the time of diagnosis was 42.7 (range, 16.1–67.5) years. The 10‐year probability of survival was 36.1% (range, 11.5–62%). Seven patients (33%) belonged to clustered MEN1 families. The spectrum of associated lesions in patients with Th‐NET was not statistically different from the spectrum of the remainder of the cohort. Various endocrine markers were high, but none were sensitive or specific enough to be useful for Th‐NET detection. CT‐scan and MRI were always positive at the time of diagnosis. No particular mutation was found to be associated with Th‐NET. Five cases underwent prophylactic thymectomy without success.ConclusionsSeveral end points may be helpful for future guidelines: (1) earlier detection of Th‐NET in MEN1 patients is required; (2) screening of both sexes is necessary; (3) a prospective study comparing MRI vs. CT scan in yearly screening for Th‐NET is needed; (4) a reinforced screening program must be established for patients who belong to clustered families; and (5) thymectomies must be performed in specialized centers.
Late Pleistocene sea-level oscillations (MIS 10–2) recorded in shallow marine and coastal plain sediments of the southern Wanganui Basin, New Zealand
Authors: Hughes Glenn R. , Kennedy David M. 
Published: March 2009

AbstractThe northern Wanganui Basin, New Zealand, is one of the key global sites for understanding marine cyclic sedimentation during the Quaternary. This paper presents the first evidence of marine cyclic sedimentation from its central-southern parts. Sedimentological, micropalaeontological and palynological analyses on a 280-m-deep borehole encountered units dating back to MIS 10. The sequence includes four marine cycles spanning MIS 9–5, which are overlain by terrestrial fluvial aggradation surfaces dating from MIS 4–2. Each marine unit represents a progressively shallowing depositional environment from the mid-shelf to coastal plain. This is overlain by a terrestrial sequence of lowstand fluvial terraces. Localized fault movements appear to have influenced the sedimentary character of the sequence during MIS 7a and 5e producing basement highs which provided protection to the shoreline. The cyclothems described in this paper now extend the already extensive, previously described record from MIS 17–10 to produce a combined eustatic record of Quaternary sea level change within the basin to MIS 5. They also provide an excellent example of the sedimentary response of a coastal basin to a progressive loss of sedimentation accommodation space.