bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel cationic triblock copolymer as noncovalent coating for the separation of proteins by CE
Authors: Cao Fuhu , Zhu Xiaoxi , Luo Zhaofeng , Xing Jinxing , Shi Xiaohua , Wang Yanmei , Cheradame Hervé 
Published: September 2011

AbstractA novel noncovalent adsorbed coating for CE has been prepared and explored. This coating was based on quaternized poly(2‐(dimethylamino)ethyl methacrylate)‐block‐poly(ethylene oxide)‐block‐poly(2‐(dimethylamino)ethyl methacrylate) (QDED) triblock copolymer which was synthesized by atomic transfer radical polymerization (ATRP) in our laboratory. The polycationic polymer and the negatively charged fused‐silica surface attracted each other through electrostatic interactions and hydrogen bonds. It was demonstrated that the coated capillaries provided an electroosmotic flow with reverse direction, and the magnitude of the electroosmotic flow can be modulated by varying the molecular mass of poly(2‐(dimethylamino)ethyl methacrylate) (PDMAEMA) block and pH value of the buffer. The effects of the molecular mass of PDMAEMA block in QDED triblock copolymer and pH value of the buffer on the separation of basic proteins were investigated in detail. The triblock copolymer coatings showed higher separation efficiency, better migration time repeatability and would apply to wider range of pH than bare fused‐silica capillary when used in separating proteins. Proteins from egg white were also separated through this QDED triblock copolymer‐coated capillary. These results demonstrated that the QDED triblock copolymer coatings are suitable for analyzing biosamples.
State space approach to magnetohydrodynamic flow of perfectly conducting micropolar fluid with stretch
Authors: Ezzat Magdy A. , El‐Sapa Shreen 
Published: September 2011

SUMMARYIn this work we introduce a model of the boundary layer equations for a perfect conducting micropolar fluid with stretch, bounded by an infinite vertical flat plane surface of a constant temperature. This model is applied to study the effects of free convection currents on the flow of the fluid in the presence of a constant magnetic field. The state space technique is adopted for the solution of a one‐dimensional problem for any set of boundary conditions. The resulting formulation together with the Laplace transform techniques are applied to a thermal shock problem. The inversion of the Laplace transforms is carried out using a numerical approach. Numerical results are given and illustrated graphically for the problem. Copyright © 2011 John Wiley & Sons, Ltd.
A multilevel examination of the influence of trainee‐trainer gender dissimilarity and trainee‐classroom gender composition dissimilarity on trainee knowledge acquisition
Authors: Bell Suzanne T. , Towler Annette J. , Fisher David M. 
Published: September 2011

AbstractWe examined the extent to which a trainee's gender interacts with the gender of the trainer, as well as the gender composition of the classroom, to influence the trainee's knowledge acquisition. Hypotheses based on asymmetrical relational demography arguments were tested with a multilevel model on data collected from 1,515 predominantly Hispanic adult trainees in 115 classrooms taught by a total of 22 different trainers. Results supported an asymmetrical relational demography effect for the relationship between trainee‐trainer gender dissimilarity and knowledge acquisition, such that trainer‐trainee dissimilarity was related to knowledge acquisition for female trainees, but not for male trainees. No effects on trainee knowledge acquisition were observed for the trainee gender and classroom gender composition interaction. Exploratory analyses suggested that the racial diversity of the classroom was related to the knowledge acquisition of female trainees, but not male trainees.
Married Thai working mothers: Coping with initial part‐time doctoral study
Authors: Thinnam Thanit 
Published: September 2011

AbstractAdvanced educational attainment can “grow” a career. But acquiring a doctoral qualification adds study to existing work and family responsibilities, especially for women. This phenomenological research explores the experiences of eight Thai working mothers enrolled in the initial stage of part‐time doctoral programs in Thailand. A majority enrolled for work‐related reasons: some believe that the qualification would support their careers, others that it would help them escape their present work situation. Work can be an ally and/or an enemy of study, or there may be no relation. Workplace support is an important factor. Findings elucidate subjects' difficulties and indicate differential levels of adaptive capacity. Copers had the ability to integrate all three demands. Doctoral study impacts on work‐life balance raise unexplored issues for HRD managers.
AUTOMATIC IN SITU DETERMINATION OF FIELD CAPACITY USING SOIL MOISTURE SENSORS
Authors: Fazackerley Scott , Lawrence Ramon 
Published: September 2011

ABSTRACTField capacity is a frequently used concept in irrigation systems and agriculture. Although there is some debate on how field capacity is defined, it is generally accepted as being the upper limit on the available water that is stored in a soil profile that can be held against the pull of gravity. Standard methods for determining field capacity require waiting two days before measuring volumetric water content after raising the water content above field capacity. This is time‐consuming and subject to natural water inputs during the monitoring period. The contribution of this work is a method for rapidly estimating field capacity in situ using volumetric water sensors, a specific irrigation schedule, and a nonlinear curve fitting model that predicts field capacity using a drainage model. The result allows for determining field capacity rapidly with very good accuracy and can be used with automatic irrigation systems in landscaping and agriculture. Copyright © 2011 John Wiley & Sons, Ltd.
Proteoglycan 4: A dynamic regulator of skeletogenesis and parathyroid hormone skeletal anabolism
Authors: Novince Chad M , Michalski Megan N , Koh Amy J , Sinder Benjamin P , Entezami Payam , Eber Matthew R , Pettway Glenda J , Rosol Thomas J , Wronski Thomas J , Kozloff Ken M , McCauley Laurie K 
Published: September 2011

Abstract
Proteoglycan 4 (Prg4), known for its lubricating and protective actions in joints, is a strong candidate regulator of skeletal homeostasis and parathyroid hormone (PTH) anabolism. Prg4 is a PTH-responsive gene in bone and liver. Prg4 null mutant mice were used to investigate the impact of proteoglycan 4 on skeletal development, remodeling, and PTH anabolic actions. Young Prg4 mutant and wild-type mice were administered intermittent PTH(1–34) or vehicle daily from 4 to 21 days. Young Prg4 mutant mice had decreased growth plate hypertrophic zones, trabecular bone, and serum bone formation markers versus wild-type mice, but responded with a similar anabolic response to PTH. Adult Prg4 mutant and wild-type mice were administered intermittent PTH(1–34) or vehicle daily from 16 to 22 weeks. Adult Prg4 mutant mice had decreased trabecular and cortical bone, and blunted PTH-mediated increases in bone mass. Joint range of motion and animal mobility were lower in adult Prg4 mutant versus wild-type mice. Adult Prg4 mutant mice had decreased marrow and liver fibroblast growth factor 2 (FGF-2) mRNA and reduced serum FGF-2, which were normalized by PTH. A single dose of PTH decreased the PTH/PTHrP receptor (PPR), and increased Prg4 and FGF-2 to a similar extent in liver and bone. Proteoglycan 4 supports endochondral bone formation and the attainment of peak trabecular bone mass, and appears to support skeletal homeostasis indirectly by protecting joint function. Bone- and liver-derived FGF-2 likely regulate proteoglycan 4 actions supporting trabeculae formation. Blunted PTH anabolic responses in adult Prg4 mutant mice are associated with altered biomechanical impact secondary to joint failure. © 2012 American Society for Bone and Mineral Research
Mindfulness and modification therapy for behavioral dysregulation: results from a pilot study targeting alcohol use and aggression in women
Authors: Wupperman Peggilee , Marlatt G. Alan , Cunningham Amy , Bowen Sarah , Berking Matthias , Mulvihill‐Rivera Nicole , Easton Caroline 
Published: September 2011

AbstractObjectives: Increasing evidence suggests that deficits in mindfulness (awareness, attentiveness, and acceptance of the present moment) play a role in a range of disorders involving behavioral dysregulation. This paper adds to that literature by describing a transdiagnostic psychotherapy (Mindfulness & Modification Therapy; MMT) developed to target behavioral dysregulation. Design: An open‐treatment pilot‐trial investigated the feasibility, acceptability, and pre‐post effects of MMT targeting women (N = 14) court‐referred for alcohol abuse/dependence and aggression. Results: Pre‐post comparisons revealed significant decreases in alcohol use, drug use, and aggression. In addition, the retention rate was 93%. Conclusion: Preliminary evidence suggests that MMT is a feasible and acceptable treatment that decreases dysregulated behaviors such as substance use and aggression, while also potentially increasing retention. © 2011 Wiley Periodicals, Inc. J Clin Psychol 67:1–17, 2011.
Arachidonic acid metabolism via cytosolic phospholipase A2α induces cytotoxicity in niemann–pick disease type C cells
Authors: Nakamura Hiroyuki , Yasufuku Kana , Makiyama Tomohiko , Matsumoto Ikiru , Fujino Hiromichi , Murayama Toshihiko 
Published: September 2011

AbstractNiemann–Pick disease type C (NPC) is a neurodegenerative lipid storage disorder caused by mutations in NPC1 or NPC2 genes. Loss of function of either protein results in the endosomal accumulation of cholesterol and other lipids. Here, we report that NPC1‐deficient Chinese hamster ovary cells exhibit increased release of arachidonic acid (AA) and synthesis of prostaglandin E2 compared with wild‐type cells. The enhanced release of AA was inhibited by both treatment with the selective inhibitor of cytosolic phospholipase A2α (cPLA2α) and cultivation in lipoprotein‐deficient medium. There was no difference in the expression of both cyclooxygenase‐1 and ‐2 between NPC cells and wild‐type cells. U18666A, a cholesterol transport‐inhibiting agent commonly used to mimic NPC, also increased the release of AA in L929 mouse fibrosarcoma cells. Furthermore, U18666A‐induced formation of reactive oxygen species (ROS) resulted in the induction of cell death and cell cycle delay/arrest in L929 cells. Interestingly, these responses induced by U18666A were much weaker in cPLA2α knockdown L929 cells. These results suggest that cPLA2α‐AA pathway plays important roles in the cytotoxicity and the ROS formation in NPC cells. J. Cell. Physiol. 227: 2847–2855, 2012. © 2011 Wiley Periodicals, Inc.
Perfusion fraction of diffusion‐weighted MRI for predicting the presence of blood supply in ovarian masses
Authors: Morita Satoru , Kojima Shinya , Hirata Masami , Suzuki Kazufumi , Ueno Eiko 
Published: September 2011

AbstractPurpose:To evaluate whether perfusion fraction (PF) calculated with diffusion‐weighted magnetic resonance imaging (MRI) predicts the presence of blood supply in ovarian masses.Materials and Methods:PFs of 92 ovarian lesions in 53 patients administered gadolinium were retrospectively calculated with diffusion‐weighted images at b‐values of 0, 500, and 1000 sec/mm2. PFs were compared between ovarian lesions, except for fat, with (n = 21) or without contrast enhancement (n = 57), using Student's t‐test and receiver operating characteristics (ROC) curve analysis. Lesion enhancement rates of contrast‐enhanced images at 30 and 180 seconds after gadolinium injection (ER30sec and ER180sec) and PFs were compared using Pearson's correlation coefficient.Results:PFs of the lesions with contrast enhancement were significantly higher than those without contrast enhancement (0.22 ± 0.09 and 0.02 ± 0.08, respectively, P < 0.0001). The ROC curve identified the best cutoff point for PF at 0.135 (95.2% sensitivity and 94.7% specificity) as a predictor of the contrast enhancement effect. The area under the ROC curve was 0.984. PF correlated moderately with ER30sec (0.62, y = 0.13x + 0.04, P < 0.0001) and ER180sec (0.74, y = 0.13x + 0.03, P < 0.0001).Conclusion:PF calculated with diffusion‐weighted images can potentially predict blood supply in ovarian masses. J. Magn. Reson. Imaging 2011. © 2011 Wiley Periodicals, Inc.
High‐resolution MRI of excised human prostate specimens acquired with 9.4T in detection and identification of cancers: Validation of a technique
Authors: Fan Xiaobing , Haney Chad R. , Agrawal Garima , Pelizzari Charles A. , Antic Tatjana , Eggener Scott E. , Sethi Ila , River Jonathan N. , Zamora Marta , Karczmar Gregory S. , Oto Aytekin 
Published: September 2011

AbstractPurpose:To evaluate feasibility of high‐resolution, high‐field ex vivo prostate magnetic resonance imaging (MRI) as an aid to guide pathologists' examination and develop in vivo MRI methods.Materials and Methods:Unfixed excised prostatectomy specimens (n = 9) were obtained and imaged immediately after radical prostatectomy under an Institutional Review Board‐approved protocol. High‐resolution T2‐weighted (T2W) MRI of specimens were acquired with a Bruker 9.4 T scanner to correlate with whole‐mount histology. Additionally, T2 and apparent diffusion coefficient (ADC) maps were generated.Results:By visual inspection of the nine prostate specimens imaged, high‐resolution T2W MRI showed improved anatomical detail compared to published low‐resolution images acquired at 4 T as published by other investigators. Benign prostatic hyperplasia, adenocarcinomas, curvilinear duct architecture distortion due to adenocarcinomas, and normal radial duct distribution were readily identified. T2 was ≈10 msec longer (P < 0.03) and the ADC was ≈1.4 times larger (P < 0.002) in the normal peripheral zone compared to the peripheral zone with prostate cancer.Conclusion:Differences in T2 and ADC between benign and malignant tissue are consistent with in vivo data. High‐resolution, high‐field MRI has the potential to improve the detection and identification of prostate structures. The protocols and techniques developed in this study could augment routine pathological analysis of surgical specimens and guide treatment of prostate cancer patients. J. Magn. Reson. Imaging 2011. © 2011 Wiley‐Liss, Inc.
Quantitative assessment of liver fat with magnetic resonance imaging and spectroscopy
Authors: Reeder Scott B. , Cruite Irene , Hamilton Gavin , Sirlin Claude B. 
Published: September 2011

AbstractHepatic steatosis is characterized by abnormal and excessive accumulation of lipids within hepatocytes. It is an important feature of diffuse liver disease, and the histological hallmark of nonalcoholic fatty liver disease (NAFLD). Other conditions associated with steatosis include alcoholic liver disease, viral hepatitis, human immunodeficiency virus (HIV) and genetic lipodystrophies, cystic fibrosis liver disease, and hepatotoxicity from various therapeutic agents. Liver biopsy, the current clinical gold standard for assessment of liver fat, is invasive and has sampling errors, and is not optimal for screening, monitoring, clinical decision‐making, or well suited for many types of research studies. Noninvasive methods that accurately and objectively quantify liver fat are needed. Ultrasound (US) and computed tomography (CT) can be used to assess liver fat but have limited accuracy as well as other limitations. Magnetic resonance (MR) techniques can decompose the liver signal into its fat and water signal components and therefore assess liver fat more directly than CT or US. Most MR techniques measure the signal fat‐fraction (the fraction of the liver MR signal attributable to liver fat), which may be confounded by numerous technical and biological factors and may not reliably reflect fat content. By addressing the factors that confound the signal fat‐fraction, advanced MR techniques measure the proton density fat‐fraction (the fraction of the liver proton density attributable to liver fat), which is a fundamental tissue property and a direct measure of liver fat content. These advanced techniques show promise for accurate fat quantification and are likely to be commercially available soon. J. Magn. Reson. Imaging 2011;. © 2011 Wiley‐Liss, Inc.
A new monoclonal antibody, 4F2, specific for the oligodendroglial cell lineage, recognizes ATP‐dependent RNA helicase Ddx54: Possible association with myelin basic protein
Authors: Ueki Toshiyuki , Tsuruo Yoshihiro , Yamamoto Yuta , Yoshimura Kazunori , Takanaga Hiromi , Seiwa Chika , Motojima Kiyoto , Asou Hiroaki , Yamamoto Masahiro 
Published: September 2011

AbstractRecent research in neural development has highlighted the importance of markers to discriminate phenotypic alterations of neural cells at various developmental stages. We isolated a new monoclonal antibody, 4F2, which was shown to be specific for an oligodendrocyte lineage. In primary cultures of oligodendroglial and mixed neural cells, the 4F2 antibody labeled a large proportion of Sox2+, Sox10+, A2B5+, NG2+, Olig2+, O4+, and myelin basic protein (MBP)+ cells but did not label any GFAP+ or NeuN+ cells. In immunohistochemisty of rat embryos, the 4F2 antibody labeled a portion of neuroepithelial cells of the neural tube at embryonic day 9. The 4F2‐positive cells were located initially in the ventricular zone as Musashi1+ Tuj1− populations and distributed throughout the striatum; thereafter, they populated the whole brain and spinal cord. These cells showed ramified processes during embryonal development. The 4F2 antigen was associated with all four isoforms of MBP in coimmunoprecipitation experiments using brain homogenates or cell lysates of cultured oligodendrocytes. Immunoscreening of a brain cDNA library identified the antigen as DEAD (Asp‐Glu‐Ala‐Asp) box polypeptide 54 (Ddx54), a member of the DEAD box family of RNA helicases involved in RNA metabolism, transcription, and translation. Cotransfection of the Ddx54 gene with MBP isoform genes increased the nuclear localization of the 21.5‐kDa MBP isoform, which has been reported to function as a nuclear signal transduction molecule. These data indicate that Ddx54 might be not only a useful marker for investigating the ontogeny of oligodendrocytes but also an important factor in oligodendrocyte differentiation and myelination. Journal of Neuroscience Research (2011) © 2011 Wiley Periodicals, Inc.
Dynamic loss of surface‐expressed AMPA receptors in mouse cortical and striatal neurons during anesthesia
Authors: Carino Charlene , Fibuch Eugene E. , Mao Li‐Min , Wang John Q. 
Published: September 2011

AbstractIonotropic glutamate receptors, especially the α‐amino‐3‐hydroxy‐5‐methylisoxazole‐4‐propionic acid (AMPA) receptor subtype, undergo dynamic trafficking between the surface membrane and intracellular organelles. This trafficking activity determines the efficacy and strength of excitatory synapses and is subject to modulation by changing synaptic inputs. Given the possibility that glutamate receptors in the central nervous system might be a sensitive target of anesthetic agents, this study investigated the possible impact of anesthesia on trafficking and subcellular expression of AMPA receptors in adult mouse brain neurons in vivo. We found that anesthesia induced by a systemic injection of pentobarbital did not alter total protein levels of three AMPA receptor subunits (GluR1–3) in cortical neurons. However, an anesthetic dose of pentobarbital reduced GluR1 and GluR3 proteins in the surface pool and elevated these proteins in the intracellular pool of cortical neurons. The similar redistribution of GluR1/3 was observed in mouse striatal neurons. Pentobarbital did not significantly alter GluR2 expression in the two pools. Chloral hydrate at an anesthetic dose also reduced surface GluR1/3 expression and increased intracellular levels of these proteins. The effect of pentobarbital on subcellular distribution of AMPA receptors was reversible. Altered subcellular distribution of GluR1/3 returned to normal levels after the anesthesia subsided. These data indicate that anesthesia induced by pentobarbital and chloral hydrate can alter AMPA receptor trafficking in both cortical and striatal neurons. This alteration is characterized by the concurrent loss and addition of GluR1/3 subunits in the respective surface and intracellular pools. Journal of Neuroscience Research (2011) © 2011 Wiley Periodicals, Inc.
Measuring system optimizes the running training
Authors: Hinz Oliver , Wenzlau Carsten 
Published: September 2011

AbstractThe most popular types of sport all over the world are walking and running. Many sportsmen have ambitions to reach a higher level. Others have even made their passion into their occupation. In all cases it needs a lot of training to reach the ambitious targets. A laser welded step sensor is able to support the running persons – indoor and outdoor.
Synthesis, Assembled Structures, and Optoelectronic Properties of Amphiphilic Block Copolymers Containing Anthracene Units in the Main Chain
Authors: Mori Hideharu , Tanaka Hiromi 
Published: September 2011

AbstractNovel amphiphilic block copolymers containing the anthracene unit in the main chain are synthesized by reversible addition‐fragmentation chain transfer (RAFT) polymerization of a cyclic monomer, 10‐methylene‐9,10‐dihydroanthryl‐9‐spirophenylcyclopropane (MDS). A hydrophilic macro‐chain transfer agent is prepared by RAFT polymerization of 2‐hydroxyethyl acrylate (HEA). The polymerizations of MDS using the poly(HEA) afford amphiphilic block copolymers, in which the hydrophobic poly(MDS) segment has a perfectly alternating structure composed of an anthracene unit and a styrene unit, owing to the ring‐opening polymerization system. Characteristic assembled structures and optoelectronic properties of the amphiphilic block copolymers are investigated in various selective solvents.
Proteomic analysis of placentas from cloned cat embryos identifies a set of differentially expressed proteins related to oxidative damage, senescence and apoptosis
Authors: Bang Jae‐Il , Bae Dong‐Won , Lee Hyo‐Sang , Deb Gautam K. , Kim Myeong‐Ok , Sohn Sea‐Hwan , Han Chang‐Hee , Kong Il‐Keun 
Published: September 2011

AbstractProduction of cloned mammals by somatic cell nuclear transfer is associated with functional and structural abnormalities of placentation and with abnormal fetal development. A proteomic analysis was performed in domestic cats (Felis catus) to compare cloned term placentas (CTP) obtained from cesarean section (CS) to control placentas obtained from CS or vaginal delivery. The expression of 20 proteins was altered in CTP (p<0.05) compared to control placentas. The two control groups showed that the method of delivery, vaginal delivery or CS, did not affect protein expression (p>0.05). A total of 13 proteins were up‐regulated in CTP, including apoptosis‐related cathepsin D (CD), annexin A1 and heat shock protein 27 (HSP 27), and seven proteins were down‐regulated in CTP, including prohibitin (PHB). The expression of PHB and CD was confirmed by Western blotting and immunofluorescence staining. The abnormal expression of PHB and CD correlated with the generation of reactive oxygen species, leading to decreased mitochondrial membrane potential and telomeric DNA, which are associated with cellular senescence and apoptosis. In summary, a specific pattern of abnormal protein expression is associated with the impaired development and functions of cloned placentas and hence with decreased fetal viability. Strategies aimed at restoring normal placental protein expression may increase the efficiency of somatic cell nuclear transfer and transgenic cat production and help restore endangered species.