bluuebunny/crossref_metadata_2025_split huggingface dataset:
Grippeimpfstoff‐Herstellung in Hühnereiern. Der jährliche Zyklus
Authors: Neumeier Elisabeth 
Published: February 2011

AbstractDie Zusammensetzung des aus drei Virusstämmen hergestellten Grippeimpfstoffs ändert sich jedes Jahr und wird zentral von der Weltgesundheitsorganisation (WHO) festgelegt. Die WHO veröffentlicht im Februar die Empfehlung für die Zusammensetzung des Impfstoffs für die nördliche Hemisphäre. Von einem der WHO‐Referenzlabore werden die Referenzstämme für die Impfstoffherstellung bereitgestellt. Influenzaviren werden für die Mehrzahl der zugelassenen Grippe‐Impfstoffe zunächst in bebrüteten Hühnereiern vermehrt, dann geerntet, gereinigt, inaktiviert und zu Fertigimpfstoff verarbeitet. Vom Eingang der Referenzstämme bis zur Auslieferung des fertigen Grippe‐Impfstoffs stehen den Herstellern in der Regel sechs bis sieben Monate zur Verfügung.
Strategien für die Entwicklung neuartiger Grippemittel. Neue Wirkstoffstrategien gegen Influenzaviren
Authors: Steinmetzer Torsten 
Published: February 2011

AbstractIn den letzten Jahren wurden viele potentielle Targets für die Entwicklung neuer Grippemittel gefunden. Inzwischen sind einzelne Verbindungen in klinischer Entwicklung, für die Mehrzahl der Zielstrukturen wurden jedoch nur sporadisch erste Wirkstoffe beschrieben. Besonders bei den Verbindungen, die gegen virale Targets gerichtet sind, wird in der Regel eine mehr oder weniger stark ausgeprägte Spezifität für bestimmte Stämme beobachtet. Neben der geringen Wirksamkeit auf andere Stämme muss auch immer mit dem raschen Auftreten von Resistenzen gerechnet werden. Ein möglicher Ausweg könnte die Entwicklung alternativer Wirkstoffe gegen humane Zielstrukturen sein, die für die Replikation der Influenzaviren essentiell sind.
BAL30072 – ein Eisen‐komplexierendes β‐Lactam‐Antibiotikum gegen multiresistente Gram‐negative Bakterien
Authors: Schmitz Jens 
Published: February 2011

AbstractObwohl gerade bei den Gram‐negativen Bakterien in den letzten Jahren vermehrt Resistenzen auftreten, herrscht ein großer Mangel an neuen Antibiotika gegen diese Erregergruppe. Ende November 2010 gab Basilea Pharmaceutica bekannt, eine Phase‐I‐Studie des von ihnen entwickelten β‐Lactam‐Antibiotikums BAL30072 initiiert zu haben, das in In‐vitro‐Untersuchungen eine gute Aktivität gegen multiresistente Gram‐negative Bakterien aufweist.
Initial testing (stage 1) of the polyamine analog PG11047 by the pediatric preclinical testing program
Authors: Smith Malcolm A. , Maris John M. , Lock Richard , Kolb E. Anders , Gorlick Richard , Keir Stephen T. , Carol Hernan , Morton Christopher L. , Reynolds C. Patrick , Kang Min H. , Houghton Peter J. 
Published: February 2011

AbstractBackgroundPG11047 is a novel conformationally restricted analog of the natural polyamine, spermine that lowers cellular endogenous polyamine levels and competitively inhibits natural polyamine functions leading to cancer cell growth inhibition. The activity of PG11047 was evaluated against the PPTP's in vitro and in vivo panels.ProceduresPG11047 was evaluated against the PPTP in vitro panel using 96 hr exposure at concentrations ranging from 10 nM to 100 µM. It was tested against the PPTP in vivo panels at a dose of 100 mg/kg administered by the intraperitoneal route weekly for 6 weeks.ResultsIn vitro PG11047 demonstrated a concentration–response pattern consistent with cytostatic activity. The median EC50 for PG11047 was 71 nM. Cell lines of the Ewing sarcoma panel had a lower median EC50 value compared to the remaining cell lines in the panel, while cell lines of the neuroblastoma panel had a higher median EC50 value. In vivo PG11047 induced significant differences in EFS distribution compared to control in 5 of 32 (15.6%) of the evaluable solid tumor xenografts and in 0 of 7 (0%) of the evaluable ALL xenografts. The single case of tumor regression occurred in an ependymoma xenograft.ConclusionsFurther pediatric development of PG11047 will require better defining a target population and identifying combinations for which there is a tumor‐selective cytotoxic effect. The regression observed for an ependymoma xenograft and the exquisite sensitivity of some Ewing sarcoma cell lines to the antiproliferative effects of PG11047 provide leads for further preclinical investigations. Pediatr Blood Cancer 2011; 57: 268–274. © 2011 Wiley‐Liss, Inc.
Initial testing of lenalidomide by the pediatric preclinical testing program
Authors: Reynolds C. Patrick , Kang Min H. , Keir Stephen T. , Gorlick Richard , Kolb E. Anders , Lock Richard , Maris John M. , Carol Hernan , Morton Christopher L. , Billups Catherine A. , Smith Malcolm A. , Houghton Peter J. 
Published: February 2011

AbstractBackgroundLenalidomide, a novel immunomodulatory agent, is reported to modulate stem cell differentiation, and have direct antiproliferative activity as well as inhibit inflammation and hyperalgesia. On the basis of this varied pharmacological profile, lenalidomide is under investigation as a treatment for a range of oncologic indications.ProceduresLenalidomide was evaluated against the PPTP in vitro panel using 96‐hr exposure at concentrations ranging from 1 nM to 10 µM. It was tested against the PPTP in vivo panels at a dose of 30 mg/kg administered orally (PO) once daily for a planned for 6 weeks.ResultsIn vitro activity was not observed at concentrations up to 10 µM. Lenalidomide was well tolerated, and induced significant differences in EFS distribution compared to control in 7 of 37 (18.9%) of the evaluable solid tumor xenografts and in 0 of 8 (0%) of the evaluable ALL xenografts. The best response in the solid tumor panel was PD2 [progressive disease with growth delay (EFS T/C > 1.5)], observed in 4 of 37 (10.8%) solid tumor xenografts. A single ALL xenograft showed a PD2 response.ConclusionsDirect antiproliferative effects of lenalidomide were not observed in vitro. In vivo lenalidomide demonstrated low activity against tumors in immune‐deficient mice. Our results suggest that lenalidomide's utility in the pediatric clinical setting may depend upon its ability to induce antitumor activity through effects on host immune and stromal cells rather than through direct effects on tumor cells. Pediatr Blood Cancer 2011; 57: 606–611. © 2011 Wiley‐Liss, Inc.
The mediating effects of family functioning on psychosocial outcomes in healthy siblings of children with sickle cell disease
Authors: Gold Jeffrey I. , Treadwell Marsha , Weissman Lina , Vichinsky Elliott 
Published: February 2011

AbstractBackgroundChildren with siblings coping with chronic illness experience stresses and disruptions in daily life as families work together to care for the affected child. Research suggests that children and adolescents with sickle cell disease (SCD) may be at risk for adjustment problems, impaired psychosocial functioning, and reduced quality of life. These potential stressors affect the child with SCD as well as their caregivers and other family members. This study examined the role of family functioning on the psychosocial functioning of healthy siblings of children with SCD.ProcedureParticipants were 65 healthy African–American siblings of children with SCD with a mean age of 11.19 years (range: 7–16) and their primary caregiver. Caregivers completed questionnaires assessing family functioning and child adjustment including demographic surveys, the Family Relations Scale (FRS), and the Child Behavior Checklist (CBCL).ResultsIncreased number of emergency room visits (β = −0.28, P < 0.05) predicted poor psychosocial adjustment in siblings. Family functioning mediated this effect (β = 0.27; P < 0.05). High levels of family expressiveness (total score, r = −0.34; P < 0.01), support (total score, r = −0.54; P = 0.001), and low levels of family conflict (total score, r = 0.41; P < 0.001) were associated with improved adjustment among healthy siblings of children with SCD.ConclusionsAwareness of the possible negative psychosocial outcomes of living with a sister or a brother with SCD is important for clinicians. In particular, interventions that focus on family expressiveness, support, and conflict are indicated for this population. Pediatr Blood Cancer 2011; 57: 1055–1061. © 2011 Wiley‐Liss, Inc.
Sickle cell disease in California: Sociodemographic predictors of emergency department utilization
Authors: Wolfson Julie A. , Schrager Sheree M. , Khanna Rachna , Coates Thomas D. , Kipke Michele D. 
Published: February 2011

AbstractBackgroundPatients with sickle cell disease (SCD) visit emergency departments (EDs) in rates leading to a significant health system burden. However, limited comprehensive evaluations of utilization patterns have been published using data connecting visits to patients across facilities. This study aims to examine sociodemographic predictors of ED utilization in SCD.ProcedureThis retrospective cohort study employed 2007 data from the California Office of Statewide Health Planning and Development (OSHPD). Data included all ED encounters from California hospitals; identifiers connected each visit to an individual patient, across all facilities in the state. Multivariate regression techniques evaluated sociodemographic predictors of utilization while adjusting for confounding variables.ResultsIn 2007, 2,920 California patients with SCD made 16,364 ED visits. Adults ≥21 years of age had higher ED visit rates than children and were more likely to both be in the highest tier of users and visit multiple facilities. Patients living further from a self‐identified provider of comprehensive SCD care had higher rates of ED visits and a lower likelihood of hospitalization from the ED. Publicly insured patients had higher rates of ED visits and were more likely to be in the highest tier of users than were the privately insured or uninsured.ConclusionsAdulthood ≥21 years of age, distance from comprehensive SCD care, and insurance status are significant predictors of ED utilization in SCD. As a routine source of care decreases ED utilization, these findings prompt concern that these factors act as barriers to accessing comprehensive SCD care. Pediatr Blood Cancer 2012; 58: 66–73. © 2011 Wiley Periodicals, Inc.
The impact of the 2009 H1N1 influenza pandemic on pediatric patients with sickle cell disease
Authors: George Alex , Benton Jennifer , Pratt Jesse , Kim Mi‐Ok , Kalinyak Karen A. , Kalfa Theodosia A. , Joiner Clinton H. 
Published: February 2011

AbstractBackgroundRespiratory infections are associated with clinically significant illness in patients with sickle cell disease (SCD). The 2009 H1N1 pandemic was perceived as a significant threat to this population.MethodsWe undertook a chart review of all patients with SCD followed at our institution to identify those with confirmed H1N1 infection. Further chart and laboratory data was collected on affected patients to analyze clinical courses and the factors that correlated with disease severity.ResultsApproximately half of the patients with confirmed H1N1 infection were managed successfully on an outpatient basis with oseltamivir therapy. Among the patients admitted, the most common diagnosis was acute chest syndrome (ACS). Most admitted patients had uncomplicated clinical courses, with a median length of admission of 3 days and no mortality or requirement for mechanical ventilation. A past history of ACS or reactive airway disease correlated with a higher rate of admission and of ACS incidence during the acute illness. Chronic transfusion therapy or hydroxyurea therapy with high hemoglobin F levels had a strong inverse correlation with incidence of ACS.ConclusionsOur results indicate that that in general the impact of the H1N1 influenza pandemic on patients with SCD was mild but that past clinical history correlated with the severity of illness. Additionally, effective hydroxyurea therapy and chronic transfusion therapy appeared to be protective against the incidence of ACS. Our results suggest guidelines for the management of patients with SCD during future influenza pandemics as well as during seasonal influenza epidemics. Pediatr Blood Cancer 2011; 57: 648–653. © 2011 Wiley‐Liss, Inc.
The Association of minor congenital anomalies and childhood cancer
Authors: Durmaz Asude , Durmaz Burak , Kadioglu Bengu , Aksoylar Serap , Karapinar Deniz , Koturoglu Guldane , Orman Mehmet N. , Ozkinay Ferda , Cogulu Ozgur 
Published: February 2011

AbstractBackgroundAlthough the association of some congenital malformations and specific genetic syndromes is well understood, the association between minor anomalies and cancer is not well known. In recent years some researchers have reported studies establishing this association in different types of cancer. In this study, we aimed to investigate the prevalence and patterns of age‐independent minor anomalies in childhood cancer patients.ProcedureTwo hundred patients with various types of cancer and 200 healthy controls were examined by two different medical geneticists for minor anomalies who evaluated all the cases and controls simultaneously. Besides minor anomalies, information on the consanguinity between the parents and occurrence of cancer in relatives were also recorded. The types of minor anomalies in different types of cancer, the number of minor anomalies in patients and controls, the association between cancer and the occurrence of different types of minor anomalies were also evaluated.ResultsThe consanguinity and the history of cancer in relatives were significantly more prevalent in patients (P = 0.04 and P < 0.001, respectively). The number of minor anomalies in patients were significantly higher compared to the controls (P < 0.01). Particularly, the presence of hypertelorism, high‐arched palate (approximately 40‐fold higher, 95% CI: 12.895–125.037) and hand‐foot anomalies were found to be more prevalent in patients having cancer compared to the controls.ConclusionThe common pathways during the embryogenesis may play a role in the development of cancer. The presence and the combination of minor anomalies seem to be associated with a higher prevalence of cancer. Pediatr Blood Cancer 2011;56:1098–1102. © 2011 Wiley‐Liss, Inc.
Mussel‐mimetic tissue adhesive for fetal membrane repair: a standardized ex vivo evaluation using elastomeric membranes
Authors: Haller C. M. , Buerzle W. , Brubaker C. E. , Messersmith P. B. , Mazza E. , Ochsenbein‐Koelble N. , Zimmermann R. , Ehrbar M. 
Published: February 2011

AbstractObjectiveIatrogenic preterm premature rupture of membranes (iPPROM), the main complication of invasive interventions in the prenatal period, seriously limits the benefit of diagnostic or surgical prenatal procedures. This study aimed to evaluate preventive plugging of punctured fetal membranes in an ex vivo situation using a new mussel‐mimetic tissue adhesive (mussel glue) to inhibit leakage.MethodsA novel biomechanical test device that tests the closure of injured membranes under near‐physiological conditions was used. Mussel glue, a poly(ethylene glycol)‐based hydrogel, was used to seal membrane defects of up to 3 mm in mechanically well‐defined elastomeric membranes with three different degrees of stiffness.ResultsElastomeric test membranes were successfully employed for testing mussel glue under well‐defined conditions. Mussel glue plugs were distended by up to 94%, which translated to an improved sealing efficiency on elastomeric membranes with high stiffness. For the stiffest membrane tested, a critical burst pressure of 48 mbar (36 mmHg) was accomplished in this ex vivo setting.ConclusionsMussel glue appears to efficiently seal membrane defects under well‐standardized ex vivo conditions. As repaired membranes resist pressures measured in amniotic cavities, mussel glue might represent a novel sealing method for iatrogenic membrane defects. Copyright © 2011 John Wiley & Sons, Ltd.
Maternal smoking and fetal lung volume—an in utero MRI investigation
Authors: Schmid Maximilian , Kasprian Gregor , Marschalek Julian , Posch Angela , Balassy Csilla , Prayer Daniela 
Published: February 2011

AbstractObjectiveTo investigate whether fetal lung volume and fetal lung volume growth over gestation are different in mothers who reported smoking during pregnancy compared to non‐smoking controls.MethodCross‐sectional retrospective study of the data of 200 consecutive singleton pregnancies that underwent magnetic resonance imaging (MRI). Fetal lung volumes of 32 fetuses of mothers who reported smoking during pregnancy were compared to 168 fetuses of non‐smoking controls.ResultsCases reported smoking between 2 and 30 cigarettes per day. The mean number of cigarettes per day for cases was 10.2 ± 6.1. After logarithmic transformation, lung volumes showed a linear increase with gestational age (r2 = 0.7). Multiple regression analysis revealed a significant influence of gestational age (p < 0.0001) but not maternal smoking status (p = 1.0) on fetal lung volumes. Comparison of the relative difference between observed and predicted lung volumes for both groups by means of unpaired t‐test revealed no significant influence of maternal smoking status on this variable (p = 0.9).ConclusionsFetuses of mothers who reported smoking during pregnancy show similar lung volumes and lung volume growth in MRI compared to non‐smoking controls. Our data suggest that a reduced lung volume is not responsible for the increase in respiratory symptoms associated with prenatal smoking. Copyright © 2011 John Wiley & Sons, Ltd.
Photovoltaics literature survey (no. 84)
Authors: Shrestha Santosh 
Published: February 2011

AbstractIn order to help keep readers up‐to‐date in the field, each issue of Progress in Photovoltaics will contain a list of recently published journal articles most relevant to its aims and scope. This list is drawn from an extremely wide range of journals, including IEEE Transactions on Electron Devices, Journal of Applied Physics, Applied Physics Letters, Progress in Photovoltaics and Solar Energy Materials and Solar Cells. To assist the reader, the list is separated into broad categories, but please note that these classifications are by no means strict. Also note that inclusion in the list is not an endorsement of a paper's quality. If you have any suggestions please e‐mail Santosh Shrestha at s.shrestha@unsw.edu.au. Copyright © 2011 John Wiley & Sons, Ltd.
Family disruption and academic functioning in urban, black youth
Authors: Somers Cheryl L. , Chiodo Lisa M. , Yoon Jina , Ratner Hilary , Barton Elizabeth , Delaney‐Black Virginia 
Published: February 2011

AbstractThe purpose of this study was to examine from an ecological perspective the relationships between multiple levels of family disruption and children's academic functioning in a sample of 390 urban, Black adolescents at age 14. Subjects in this cohort have been followed longitudinally since before their birth. Data from previous assessments at birth and age 7 years allowed for control of important correlates in this analyses. Examination of this unique sample, using multisource data collection procedures, provides important information about the many family disruptions among urban, low‐income families and the effects of these disruptions on academic outcomes. Results revealed several consistent findings, the most notable of which is that, when parents were married, adolescents demonstrated significantly higher grades, mediated through paternal involvement. Of all variables studied, the role of fathers in the lives of these adolescents was the most influential on academic outcomes. In this unique sample, even variables such as involvement with child protective services were not significantly related to outcomes after important control variables were factored into analyses. Results of this study have important implications for future research and for prevention and intervention efforts in urban, at‐risk samples. Emphasis is made for work in the school context, particularly for school psychologists. © 2011 Wiley Periodicals, Inc.
Closing the gap in school mental health: A community‐centered model for school psychology
Authors: Splett Joni Williams , Maras Melissa A. 
Published: February 2011

AbstractDisciplines involved in the development and/or provision of services in school mental health have recognized the promise of evidence‐based practices in meeting the growing unmet mental health needs of children, adolescents, and families in this country. As a result, School Psychology has strongly emphasized the development and dissemination of evidence‐based practices through professional development opportunities and published literature. Despite these efforts, evidence‐based practices are not implemented as widely or as effectively as intended. This article describes a community‐centered (CC) model for closing the research‐to‐practice gap in school mental health. CC models emphasize both adopting new practices successfully and improving existing practices through a process of planning, implementation, and evaluation, and an exemplar framework based in a CC model is presented and applied to school mental health. The role of school psychologists in leading schools to adopt CC models is discussed, and next steps for changes to training, practice, and research paradigms are presented. © 2011 Wiley Periodicals, Inc.
Time to articulate a vision for the future of plant proteomics – A global perspective: An initiative for establishing the International Plant Proteomics Organization (INPPO)
Authors: Agrawal Ganesh Kumar , Job Dominique , Zivy Michel , Agrawal Vishwanath P. , Bradshaw Ralph A. , Dunn Michael J. , Haynes Paul A. , van Wijk Klaas J. , Kikuchi Shoshi , Renaut Jenny , Weckwerth Wolfram , Rakwal Randeep 
Published: February 2011

AbstractGiven the essential role of proteomics in understanding the biology of plants, we are establishing a global plant proteomics organization to properly organize, preserve and disseminate collected information on plant proteomics. We call this organization ‘International Plant Proteomics Organization (INPPO; http://www.inppo.com).’ Ten initiatives of INPPO are outlined along with how to address them in multiple phases. As our vision is global, we sincerely hope the scientific communities around the world will come together to support and join INPPO.
Low‐SDS Blue native PAGE
Authors: Klodmann Jennifer , Lewejohann Dagmar , Braun Hans‐Peter 
Published: February 2011

AbstractSDS normally is strictly avoided during Blue native (BN) PAGE because it leads to disassembly of protein complexes and unfolding of proteins. Here, we report a modified BN‐PAGE procedure, which is based on low‐SDS treatment of biological samples prior to native gel electrophoresis. Using mitochondrial OXPHOS complexes from Arabidopsis as a model system, low SDS concentrations are shown to partially dissect protein complexes in a very defined and reproducible way. If combined with 2‐D BN/SDS‐PAGE, generated subcomplexes and their subunits can be systematically investigated, allowing insights into the internal architecture of protein complexes. Furthermore, a 3‐D BN/low‐SDS BN/SDS‐PAGE system is introduced to facilitate structural analysis of individual protein complexes without their previous purification.
Simplified enrichment of plasma membrane proteins for proteomic analyses in Arabidopsis thaliana
Authors: Zhang Zhe Jenny , Peck Scott C. 
Published: February 2011

AbstractThe plasma membrane (PM) serves as the point of contact between cells and the outside environment. As such, changes in the PM proteome are an important component of understanding cellular responses to a diverse array of stimuli. However, intricate sample handling to enrich PM proteomes by traditional methods is both technically challenging and time consuming. Here, we describe a simplified method for decreasing the representation of other membrane‐containing organelles such as the endoplasmic reticulum, plastids and mitochondria from crude microsomal membrane isolations. The decrease in other organellar proteomes results in an increase in both the total number of PM proteins and the number of spectra identified from these proteins representing the PM proteome. Therefore, this strategy represents a simple and rapid method for enriching PM proteins from Arabidopsis cell cultures for proteomic analyses.