bluuebunny/crossref_metadata_2025_split huggingface dataset:
Temporomandibular joint injuries and ankylosis in the cat
Authors: Çetinkaya M. A. 
Published: July 2012

SummaryObjective: To evaluate cause, location, treatment, and the clinical outcome of traumatic temporomandibular joint (TMJ) lesions and TMJ ankylosis in cats.Methods: Cats with TMJ injuries were included in this study. Lesions were classified as luxations, fractures of the condylar process, and intra-articular temporal bone fractures. Signalment, cause and type of injuries, treatment methods, clinical outcome, complications and joint ankylosis were assessed and evaluated statistically.Results: Temporomandibular joint lesions were observed in 82 of 161 cats with maxillofacial injuries. One hundred forty-nine TMJ lesions were determined in 112 joints. Falling was the most common cause and fractures of the condylar process were the most common types of injuries. Isolated TMJ injuries and caudal TMJ luxations were mainly caused by falling. Condylectomy was used in ankylosis, chronic luxation, reluxation and in two cases with multiple TMJ lesions. Ankylosis was observed in 10.97% of cases and was generally observed in fracture combinations of condylar process and mandibular fossa (Χ2 = 8.52; p <0.05). No significant relationship between age and development of ankylosis (Χ2 = 3.995; p >0.05) was found.Conclusion: In contrast to previous studies, traumatic TMJ lesions were observed in a considerable amount of cats with maxillofacial injuries, and fractures of the condylar process were the most common type. Lesions caused by falling were mostly simple, whereas vehicular trauma caused more complicated lesions. Ankylosis did not appear as a rare condition. Any cat with TMJ injury is susceptible to the development of ankylosis.
Recombinatorial Biases and Convergent Recombination Determine Interindividual TCRβ Sharing in Murine Thymocytes
Authors: Li Hanjie , Ye Congting , Ji Guoli , Wu Xiaohui , Xiang Zhe , Li Yuanyue , Cao Yonghao , Liu Xiaolong , Douek Daniel C. , Price David A. , Han Jiahuai 
Published: July 2012

Abstract
Overlap of TCR repertoires among individuals provides the molecular basis for public T cell responses. By deep-sequencing the TCRβ repertoires of CD4+CD8+ thymocytes from three individual mice, we observed that a substantial degree of TCRβ overlap, comprising ∼10–15% of all unique amino acid sequences and ∼5–10% of all unique nucleotide sequences across any two individuals, is already present at this early stage of T cell development. The majority of TCRβ sharing between individual thymocyte repertoires could be attributed to the process of convergent recombination, with additional contributions likely arising from recombinatorial biases; the role of selection during intrathymic development was negligible. These results indicate that the process of TCR gene recombination is the major determinant of clonotype sharing between individuals.
The Protein Tyrosine Phosphatase SHP-1 Regulates Phagolysosome Biogenesis
Authors: Gómez Carolina P. , Tiemi Shio Marina , Duplay Pascale , Olivier Martin , Descoteaux Albert 
Published: July 2012

Abstract
The process of phagocytosis and phagosome maturation involves the recruitment of effector proteins that participate in phagosome formation and in the acidification and/or fusion with various endocytic vesicles. In the current study, we investigated the role of the Src homology region 2 domain-containing phosphatase 1 (SHP-1) in phagolysosome biogenesis. To this end, we used immortalized bone marrow macrophages derived from SHP-1–deficient motheaten mice and their wild-type littermates. We found that SHP-1 is recruited early and remains present on phagosomes for up to 4 h postphagocytosis. Using confocal immunofluorescence microscopy and Western blot analyses on purified phagosome extracts, we observed an impaired recruitment of lysosomal-associated membrane protein 1 in SHP-1–deficient macrophages. Moreover, Western blot analyses revealed that whereas the 51-kDa procathepsin D is recruited to phagosomes, it is not processed into the 46-kDa cathepsin D in the absence of SHP-1, suggesting a defect in acidification. Using the lysosomotropic agent LysoTracker as an indicator of phagosomal pH, we obtained evidence that in the absence of SHP-1, phagosome acidification was impaired. Taken together, these results are consistent with a role for SHP-1 in the regulation of signaling or membrane fusion events involved in phagolysosome biogenesis.
Inhibition of Mechanistic Target of Rapamycin Promotes Dendritic Cell Activation and Enhances Therapeutic Autologous Vaccination in Mice
Authors: Amiel Eyal , Everts Bart , Freitas Tori C. , King Irah L. , Curtis Jonathan D. , Pearce Erika L. , Pearce Edward J. 
Published: July 2012

Abstract
Dendritic cells (DCs) are potent inducers of T cell immunity, and autologous DC vaccination holds promise for the treatment of cancers and chronic infectious diseases. In practice, however, therapeutic vaccines of this type have had mixed success. In this article, we show that brief exposure to inhibitors of mechanistic target of rapamycin (mTOR) in DCs during the period that they are responding to TLR agonists makes them particularly potent activators of naive CD8+ T cells and able to enhance control of B16 melanoma in a therapeutic autologous vaccination model in the mouse. The improved performance of DCs in which mTOR has been inhibited is correlated with an extended life span after activation and prolonged, increased expression of costimulatory molecules. Therapeutic autologous vaccination with DCs treated with TLR agonists plus the mTOR inhibitor rapamycin results in improved generation of Ag-specific CD8+ T cells in vivo and improved antitumor immunity compared with that observed with DCs treated with TLR agonists alone. These findings define mTOR as a molecular target for augmenting DC survival and activation, and document a novel pharmacologic approach for enhancing the efficacy of therapeutic autologous DC vaccination.
Activation-Induced Cytidine Deaminase-Initiated Off-Target DNA Breaks Are Detected and Resolved during S Phase
Authors: Hasham Muneer G. , Snow Kathy J. , Donghia Nina M. , Branca Jane A. , Lessard Mark D. , Stavnezer Janet , Shopland Lindsay S. , Mills Kevin D. 
Published: July 2012

Abstract
Activation-induced cytidine deaminase (AID) initiates DNA double-strand breaks (DSBs) in the IgH gene (Igh) to stimulate isotype class switch recombination (CSR), and widespread breaks in non-Igh (off-target) loci throughout the genome. Because the DSBs that initiate class switching occur during the G1 phase of the cell cycle, and are repaired via end joining, CSR is considered a predominantly G1 reaction. By contrast, AID-induced non-Igh DSBs are repaired by homologous recombination. Although little is known about the connection between the cell cycle and either induction or resolution of AID-mediated non-Igh DSBs, their repair by homologous recombination implicates post-G1 phases. Coordination of DNA breakage and repair during the cell cycle is critical to promote normal class switching and prevent genomic instability. To understand how AID-mediated events are regulated through the cell cycle, we have investigated G1-to-S control in AID-dependent genome-wide DSBs. We find that AID-mediated off-target DSBs, like those induced in the Igh locus, are generated during G1. These data suggest that AID-mediated DSBs can evade G1/S checkpoint activation and persist beyond G1, becoming resolved during S phase. Interestingly, DSB resolution during S phase can promote not only non-Igh break repair, but also Ig CSR. Our results reveal novel cell cycle dynamics in response to AID-initiated DSBs, and suggest that the regulation of the repair of these DSBs through the cell cycle may ensure proper class switching while preventing AID-induced genomic instability.
Decoding the Regulation of Mast Cell Exocytosis by Networks of Rab GTPases
Authors: Azouz Nurit P. , Matsui Takahide , Fukuda Mitsunori , Sagi-Eisenberg Ronit 
Published: July 2012

Abstract
Exocytosis is a key event in mast cell functions. By this process, mast cells release inflammatory mediators, contained in secretory granules (SGs), which play important roles in immunity and wound healing but also provoke allergic and inflammatory responses. The mechanisms underlying mast cell exocytosis remained poorly understood. An essential step toward deciphering the mechanisms behind exocytosis is the identification of the cellular components that regulate this process. Because Rab GTPases regulate specific trafficking pathways, we screened 44 Rabs for their functional impacts on exocytosis triggered by the FcεRI or combination of Ca2+ ionophore and phorbol ester. Because exocytosis involves the continuous reorganization of the actin cytoskeleton, we also repeated our screen in the presence of cytochalasin D that inhibits actin polymerization. In this paper, we report on the identification of 30 Rabs as regulators of mast cell exocytosis, the involvement of 26 of which has heretofore not been recognized. Unexpectedly, these Rabs regulated exocytosis in a stimulus-dependent fashion, unless the actin skeleton was disrupted. Functional clustering of the identified Rabs suggested their classification as Rabs involved in SGs biogenesis or Rabs that control late steps of exocytosis. The latter could be further divided into Rabs that localize to the SGs and Rabs that regulate transport from the endocytic recycling compartment. Taken together, these findings unveil the Rab networks that control mast cell exocytosis and provide novel insights into their mechanisms of action.
CD8 T Cells Are Essential for Recovery from a Respiratory Vaccinia Virus Infection
Authors: Goulding John , Bogue Rebecka , Tahiliani Vikas , Croft Michael , Salek-Ardakani Shahram 
Published: July 2012

Abstract
The precise immune components required for protection against a respiratory Orthopoxvirus infection, such as human smallpox or monkeypox, remain to be fully identified. In this study, we used the virulent Western Reserve strain of vaccinia virus (VACV-WR) to model a primary respiratory Orthopoxvirus infection. Naive mice infected with VACV-WR mounted an early CD8 T cell response directed against dominant and subdominant VACV-WR Ags, followed by a CD4 T cell and Ig response. In contrast to other VACV-WR infection models that highlight the critical requirement for CD4 T cells and Ig, we found that only mice deficient in CD8 T cells presented with severe cachexia, pulmonary inflammation, viral dissemination, and 100% mortality. Depletion of CD8 T cells at specified times throughout infection highlighted that they perform their critical function between days 4 and 6 postinfection and that their protective requirement is critically dictated by initial viral load and virulence. Finally, the ability of adoptively transferred naive CD8 T cells to protect RAG−/− mice against a lethal VACV-WR infection demonstrated that they are both necessary and sufficient in protecting against a primary VACV-WR infection of the respiratory tract.
Chitinase 1 Is a Biomarker for and Therapeutic Target in Scleroderma-Associated Interstitial Lung Disease That Augments TGF-β1 Signaling
Authors: Lee Chun Geun , Herzog Erica L. , Ahangari Farida , Zhou Yang , Gulati Mridu , Lee Chang-Min , Peng Xueyan , Feghali-Bostwick Carol , Jimenez Sergio A. , Varga John , Elias Jack A. 
Published: July 2012

Abstract
Interstitial lung disease (ILD) with pulmonary fibrosis is an important manifestation in systemic sclerosis (SSc, scleroderma) where it portends a poor prognosis. However, biomarkers that predict the development and or severity of SSc-ILD have not been validated, and the pathogenetic mechanisms that engender this pulmonary response are poorly understood. In this study, we demonstrate in two different patient cohorts that the levels of chitotriosidase (Chit1) bioactivity and protein are significantly increased in the circulation and lungs of SSc patients compared with demographically matched controls. We also demonstrate that, compared with patients without lung involvement, patients with ILD show high levels of circulating Chit1 activity that correlate with disease severity. Murine modeling shows that in comparison with wild-type mice, bleomycin-induced pulmonary fibrosis was significantly reduced in Chit1−/− mice and significantly enhanced in lungs from Chit1 overexpressing transgenic animals. In vitro studies also demonstrated that Chit1 interacts with TGF-β1 to augment fibroblast TGF-β receptors 1 and 2 expression and TGF-β–induced Smad and MAPK/ERK activation. These studies indicate that Chit1 is potential biomarker for ILD in SSc and a therapeutic target in SSc-associated lung fibrosis and demonstrate that Chit1 augments TGF-β1 effects by increasing receptor expression and canonical and noncanonical TGF-β1 signaling.
Perception of Patients with Amalgam Fillings about Toxicity of Mercury in Dental Amalgam
Authors: Bamise CT , Oginni Adeleke O , Adedigba Michael A , Olagundoye OO 
Published: July 2012

ABSTRACT

Objective
The objective of this study is to evaluate the awareness of patients with dental fillings about the toxicity of mercury in dental amalgam.

Materials and methods
Adult patients having at least one amalgam filling in their mouth were recruited in the Oral Diagnosis Department of OAUTHC, Ile-Ife Dental Hospital. Participants were recruited consecutively as they report in the clinic. Data were collected using a structured questionnaire developed based on standard questions from relevant publications. They were asked to indicate the type of filling material in their mouth, ingredients of the material, previous knowledge of mercury in dental amalgam and ailments due to mercury.
They were to indicate their level of agreement with filling their cavities with dental amalgam despite prior information about its mercury content.

Results
There were about 446 respondents analyzed; male, 194 (43.5%); female 252 (56.5%). Six (1.4%) and 21 (4.7%) respondents were primary and secondary schools students respectively; 15(3.4%) had no formal education while about 410 (91.9%) were either undergraduate or graduate. All of them had at least one amalgam filling. 249 (55%) participants know the type of filling on their teeth; 156 (34.5%) had the knowledge of the presence of mercury in dental amalgam while 26.1% believed mercury can cause problems in human beings. About 90 (19.9%) participants claimed to have heard about adverse reactions to dental amalgams and 34 (7.5%) of them have heard about people recovering from an illness after removal of their filling.
The level of agreement with filling their cavities with amalgam despite prior knowledge of its mercury content was 74% while 60% was observed for allowing just any material to be placed on their teeth.

Conclusion
Awareness of toxicity of mercury in dental amalgam was slightly low among the respondents studied. This may be suggested to be a reflection of nonexistent of global amalgam controversy in Nigeria.

How to cite this article
Bamise CT, Oginni AO, Adedigba MA, Olagundoye OO. Perception of Patients with Amalgam Fillings about Toxicity of Mercury in Dental Amalgam. J Contemp Dent Pract 2012;13(3):289-293.
Oral Health-Related Quality of Life (OHQoL) among Adolescents Seeking Orthodontic Treatment
Authors: Manjith CM , Karnam Srinivas Kumar , Manglam Sunil , Praveen MN , Mathur Anirudh 
Published: July 2012

ABSTRACT

Aim
The aim of this study was to assess the effect of different orthodontic treatment needs on the OHQoL of adolescents.

Materials and methods
200 subjects (100 males and 100 females), 11 to 15 years of age were recruited for the study. OHQoL was assessed with the short form of the oral health impact profile (OHIP-14), and malocclusion severity was assessed with the index of orthodontic treatment need (IOTN). The Chi-square test was used to analyse the qualitative data. The level of significance was 0.05.

Results
The more severe the malocclusion the worse was the impact on the OHQoL. Orthodontic treatment need had almost similar impact on the daily activities of both males and females. Pronunciation and taste was not significantly affected by the need for orthodontic treatment in either males or females. The proportions of orthodontic patients who found it uncomfortable to eat any food and had to interrupt their meals were significantly correlated with orthodontic treatment needs in both males and females.

Conclusion
Orthodontic treatment need had an impact on OHQoL of adolescents with no significant difference between males and females.

Clinical significance
Orthodontists should be aware of the impact caused by malocclusion and orthodontic treatment on the quality of life of the patients and should provide regular positive reinforcements to them.

How to cite this article
Manjith CM, Karnam SK, Manglam S, Praveen MN, Mathur A. Oral Health-Related Quality of Life (OHQoL) among Adolescents Seeking Orthodontic Treatment. J Contemp Dent Pract 2012;13(3):294-298.
Assessment of Skeletal and Dental Maturity Levels for a given Chronological Age among Indian Children
Authors: Murthy K Krishna , Srinivas C Nagendra , LNU Varalakshmi , Kumar C Vijay , Krishnaveni M 
Published: July 2012

ABSTRACT

Aim
The aim of this study was to evaluate the maturity levels of the individual by comparing the correlation between dental, skeletal and the chronological ages.

Materials and methods
Intraoral periapical radiographs of the complete right side of the dentition and posteroanterior radiographs of the hand and wrist were obtained from 260 subjects, ranged from 3 to 15 years of age. Evaluation of handwrist roentgenograms for skeletal age and intraoral roentgenograms for dental age was done.

Results
The findings of present study indicated that participated children were skeletally and dentally, retarded in maturation as compared with the standards except at 3 and 6 years of age. The girls were found to be skeletally more advanced than boys at all ages except 7 and 10 years of age. The dental maturity is more for girls than that of boys.

Conclusion
No significant correlation was found among skeletal age, dental age and chronological age. Dental age and skeletal age was found to be correlated at 6, 10 and 11 years in boys and 6 and 9 years of age in girls.

Clinical significance
The results of this study could enhance the diagnosis and treatment planning in orthodontics and other dental disciplines.

How to cite this article
Murthy KK, Srinivas CN, Varalakshmi, Kumar CV, Krishnaveni M. Assessment of Skeletal and Dental Maturity Levels for a given Chronological Age among Indian Children. J Contemp Dent Pract 2012;13(3):310-315.
Comparative Analysis of Postcementation Hypersensitivity with Glass Ionomer Cement and a Resin Cement: An in vivo Study
Authors: Mehta Deepak , Shetty Rohit M , Bhat Sonia , Srivatsa G , Shetty Y Bharath 
Published: July 2012

ABSTRACT

Aim
The aim of this clinical study was to compare the postoperative sensitivity of abutment teeth restored with full coverage restorations retained with either conventional glassionomer cement (GIC) or resin cement.

Materials and methods
Fifty patients received full-coverage restorations on vital abutment teeth. Of these, 25 were cemented with GIC (GC Luting and Lining cement) and the other 25 using an adhesive resin cement (Smartcem 2). A randomized single blind study was undertaken for acquiring and evaluating the data. The teeth were examined before cementation, after cementation, 24 hours postcementation and 7 days postcementation. A visual analog scale was used to help the patient rate hypersensitivity.

Results
The statistical analysis of the result was done using students paired t-test. No statistically significant difference between Smartcem 2 and GIC was observed, when tested immediately and 24 hours after cementation. Statistically significant difference was seen between Smartcem 2 and GIC when tested 7 days postcementation with a significance level of 0.05. Higher postoperative sensitivity was seen with GIC when compared to resin cement.

Conclusion
In this study, the incidence of postoperative hypersensitivity after cementation of full-crown restorations with GIC and resin cement was similar when tested immediately. However, 7 days postcementation, abutments with GIC showed higher response compared to resin cement.

Clinical significance
A self-adhesive resin cement can be the material of choice for luting if presence of postoperative sensitivity is of prime consideration. In case GIC is being used, patient should be informed about the presence of sensitivity for a more prolonged period than with resin cement.

How to cite this article
Shetty RM, Bhat S, Mehta D, Srivatsa G, Shetty YB. Comparative Analysis of Postcementation Hypersensitivity with Glass Ionomer Cement and a Resin Cement: An in vivo Study. J Contemp Dent Pract 2012;13(3): 327-331.
Assessment of Interobserver Variability in Mitotic Figure Counting in Different Histological Grades of Oral Squamous Cell Carcinoma
Authors: Prasanna MD , Yadav K Shailaja , Gonuguntla Sudhir , Velidandla Surekha Reddy , Reddy CR Charan , Bommu Sanjay Reddy 
Published: July 2012

ABSTRACT
Mitotic counting is often used for classification, grading and prognosis of tumors. The count usually stands as a decision point for treatment as well. The easiest way of counting the number of mitoses is done by screening routine H&E stained slides. However, for proper mitotic counting, certain strict protocols should be taken into consideration. This study on 30 cases of different grades of oral squamous cell carcinoma was undertaken to determine the interobserver variations in two different groups: Group1 (A1, A2), who were given certain criteria to be followed during the counting of the mitotic figures and group 2 investigators (B1, B2) who were unaware of such criteria. The paired t-test gives a correlation of 0.988 and a significant difference of 0.000 between the two investigators in group 1. The correlation was 0.650 with a significant difference of 0.058 between two investigators in-group 2, indicating that group 1 observers exhibit good interobserver agreement. The results emphasize that following of strict protocols are of great help in determining the accuracy of mitotic counting.

How to cite this article
Yadav KS, Gonuguntla S, Ealla KKR, Velidandla SR, Reddy CRC, Prasanna MD, Bommu SR. Assessment of Interobserver Variability in Mitotic Figure Counting in Different Histological Grades of Oral Squamous Cell Carcinoma. J Contemp Dent Pract 2012;13(3):339-344.