bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multi-dimensional cell-free DNA-based liquid biopsy for sensitive early detection of gastric cancer
Authors: Yu Pengfei , Chen Ping , Wu Min , Ding Guangyu , Bao Hua , Du Yian , Xu Zhiyuan , Yang Litao , Fang Jingquan , Huang Xingmao , Lai Qian , Wei Jia , Yan Junrong , Yang Shanshan , He Peng , Wu Xue , Shao Yang , Su Dan , Cheng Xiangdong 
Published: June 2024

AbstractBackgroundGastric cancer is the fifth most common cancer type. Most patients are diagnosed at advanced stages with poor prognosis. A non-invasive assay for the detection of early-stage gastric cancer is highly desirable for reducing associated mortality.MethodsWe collected a prospective study cohort of 110 stage I–II gastric cancer patients and 139 non-cancer individuals. We performed whole-genome sequencing with plasma samples and profiled four types of cell-free DNA (cfDNA) characteristics, fragment size pattern, copy number variation, nucleosome coverage pattern, and single nucleotide substitution. With these differential profiles, we developed an ensemble model to detect gastric cancer signals. Further, we validated the assay in an in-house first validation cohort of 73 gastric cancer patients and 94 non-cancer individuals and an independent second validation cohort of 47 gastric cancer patients and 49 non-cancer individuals. Additionally, we evaluated the assay in a hypothetical 100,000 screening population by Monte Carlo simulation.ResultsOur cfDNA-based assay could distinguish early-stage gastric cancer from non-cancer at an AUROC of 0.962 (95% CI: 0.942–0.982) in the study cohort, 0.972 (95% CI: 0.953–0.992) in the first validation cohort and 0.937 (95% CI: 0.890–0.983) in the second validation cohort. The model reached a specificity of 92.1% (128/139) and a sensitivity of 88.2% (97/110) in the study cohort. In the first validation cohort, 91.5% (86/94) of non-cancer individuals and 91.8% (67/73) of gastric cancer patients were correctly identified. In the second validation cohort, 89.8% (44/49) of non-cancer individuals and 87.2% (41/47) of gastric cancer patients were accurately classified.ConclusionsWe introduced a liquid biopsy assay using multiple dimensions of cfDNA characteristics that could accurately identify early-stage gastric cancer from non-cancerous conditions. As a cost-effective non-invasive approach, it may provide population-wide benefits for the early detection of gastric cancer.Trial registrationThis study was registered on ClinicalTrials.gov under the identifier NCT05269056 on March 7, 2022.
In vitro phenotypic characterisation of two genotype I African swine fever viruses with genomic deletion isolated from Sardinian wild boars
Authors: Franzoni Giulia , Fiori Mariangela S. , Mura Lorena , Carta Tania , Di Nardo Antonello , Floris Matteo , Ferretti Luca , Zinellu Susanna , Angioi Pier Paolo , Sechi Anna Maria , Carusillo Francesca , Brundu Diego , Fadda Manlio , Bazzardi Riccardo , Giammarioli Monica , Cappai Stefano , Dei Giudici Silvia , Oggiano Annalisa 
Published: June 2024

AbstractAfrican swine fever virus (ASFV) causes a devastating disease affecting domestic and wild pigs. ASF was first introduced in Sardinia in 1978 and until 2019 only genotype I isolates were identified. A remarkable genetic stability of Sardinian ASFV isolates was described, nevertheless in 2019 two wild boar isolates with a sustained genomic deletion (4342 base pairs) were identified (7303WB/19, 7212WB/19). In this study, we therefore performed in vitro experiments with monocyte-derived macrophages (moMФ) to unravel the phenotypic characteristics of these deleted viruses. Both 7303WB/19 and 7212WB/19 presented a lower growth kinetic in moMФ compared to virulent Sardinian 26544/OG10, using either a high (1) or a low (0.01) multiplicity of infection (MOI). In addition, flow cytometric analysis showed that both 7303WB/19 and 7212WB/19 presented lower intracellular levels of both early and late ASFV proteins. We subsequently investigated whether deleted virus variants were previously circulating in wild boars in Sardinia. In the four years preceding the last genotype I isolation (February 2015–January 2019), other eight wild boar isolates were collected, all belonging to p72 genotype I, B602L subgroup X, but none of them presented a sustained genomic deletion. Overall, we observed the deleted virus isolates in Sardinia only in 2019, at the end of a strong eradication campaign, and our data suggest that it might possess an attenuated phenotype in vivo. A better understanding of ASFV evolution in endemic territories might contribute to development of effective control measures against ASF.
Performance analysis of partial NOMA-based layered D2D communications
Authors: Ju Jinjuan , Sun Qiang 
Published: June 2024

AbstractConventionally, non-orthogonal multiple access (NOMA) has traditionally been implemented separately from orthogonal multiple access (OMA), aiming to improve the capacity of multi-user systems. However, a recent study has ventured beyond this conventional approach by integrating OMA and NOMA proportionally within the same system. In spite of these advancements, the consideration towards optimizing multi-user systems remains incomplete especially when user service requirements vary significantly. Therefore, this paper explores a novel layered device-to-device (D2D) partial NOMA (P-NOMA) scheme, which introduces a hybrid power-domain access method into multi-user systems. The analysis primarily focuses on evaluating both the system performance and the impact of various parameters on it. In contrast to conventional fully overlapped NOMA signals, P-NOMA signals are partially overlapped with an overlap rate that can be determined based on quality-of-service (QoS) requirements. Simulation results demonstrate that judicious utilization of P-NOMA can effectively enhance overall system performance, particularly in terms of sum rate (SR) metrics, while also flexibly accommodating diverse QoS requirements for multiple users.
Navigating intersectoral collaboration in nutrition programming: implementors’ perspectives from Assam, India
Authors: Aivalli Praveenkumar , Gilmore Brynne , Srinivas Prashanth Nuggehalli , De Brún Aoife 
Published: June 2024

Abstract
Background
There is a growing interest in the use of intersectoral collaborative (ISC) approaches to address complex health-related issues. However, relatively little empirical research exists on the challenges of implementing, fostering and sustaining these approaches. Our study explores the perceptions and experiences of programme implementers regarding the implementation of an ISC approach, focusing on a case study of nutrition programming in Assam, India.

Methods
We conducted qualitative semi-structured face-to-face in-depth interviews with eleven programme implementers from two selected districts of Assam, India. These participants were purposefully sampled to provide a comprehensive understanding of the experiences of implementing intersectoral collaboration. Following the interviews, an inductive thematic analysis was performed on the collected data.

Results
The study identified three main themes: operationalisation of ISC in daily practice, facilitators of ISC, and barriers to effective ISC. These were further broken down into six subthemes: defined sectoral mandates, leadership dynamics, interpersonal relationships and engagement, collective vision and oversight, resource allocation, and power dynamics. These findings highlight the complexity of ISC, focusing on the important structural and relational aspects at the macro, meso, and micro levels. Interpersonal relationships and power dynamics among stakeholders substantially influenced ISC formation in both the districts.

Conclusion
Despite challenges, there is ongoing interest in establishing ISC in nutrition programming, supported by political development agendas. Success relies on clarifying sectoral roles, addressing power dynamics, and engaging stakeholders systematically. Actionable plans with measurable targets are crucial for promoting and sustaining ISC, ensuring positive programme outcomes. The insights from our study provide valuable guidance for global health practitioners and policymakers dealing with similar challenges, emphasising the urgent need for comprehensive research given the lack of universally recognised policies in the realm of ISC in global health practice.
The protective role of cognitive reserve: an empirical study in mild cognitive impairment
Authors: Marselli Giulia , Favieri Francesca , Forte Giuseppe , Corbo Ilaria , Agostini Francesca , Guarino Angela , Casagrande Maria 
Published: June 2024

Abstract
Background
Mild cognitive impairment (MCI) describes an aging profile characterized by a cognitive decline that is worse than expected in normal aging but less pervasive and critical than full-blown dementia. In the absence of an effective treatment strategy, it is important to identify factors that can protect against progression to dementia. In this field, it is hypothesized that one aspect that may be a protective factor against the neurotypical outcome of dementia is cognitive reserve (CR). Cognitive reserve is the ability to maintain cognitive functionality despite accumulating brain pathology.

Objectives
The present study aimed to identify and analyze the differences in CR between healthy adults and patients with MCI. Specifically, it is hypothesized that (i) healthy older adult people have higher CR than older adult people diagnosed with MCI, and (II) CR could predict the classification of subjects into people with or without MCI.

Methods
Two hundred forty-three adults (mean age = 60.4, SD = 7.4) participated in the present study and were classified into three groups based on Petersen’s MCI criteria: healthy controls (HC), amnestic MCI (aMCI), and non-amnestic MCI (naMCI). The Cognitive Reserve Index questionnaire (CRIq) was administered to assess the level of CR,

Findings
Results showed that HC had significantly higher CR scores than participants diagnosed with aMCI and naMCI. Moreover, a binomial logistic regression suggested that low CR was a significant risk factor for the MCI diagnosis.

Conclusions
The clinical picture that emerged from the results showed that lower CR could be considered a characteristic of pathological aging, such as MCI.Public significance statement, Since the brain attempts to cope with life-related changes or pathologies, it is fundamental for both clinicians and researchers to investigate further the factors that contribute to brain resilience. As an indirect expression of brain reserve, cognitive reserve may be both a marker and a predictor of adaptive aging.
Predicting suitable habitats for foraging and migration in Eastern Indian Ocean pygmy blue whales from satellite tracking data
Authors: Ferreira Luciana C. , Jenner Curt , Jenner Micheline , Udyawer Vinay , Radford Ben , Davenport Andrew , Moller Luciana , Andrews-Goff Virginia , Double Mike , Thums Michele 
Published: June 2024

Abstract
Background
Accurate predictions of animal occurrence in time and space are crucial for informing and implementing science-based management strategies for threatened species.

Methods
We compiled known, available satellite tracking data for pygmy blue whales in the Eastern Indian Ocean (n = 38), applied movement models to define low (foraging and reproduction) and high (migratory) move persistence underlying location estimates and matched these with environmental data. We then used machine learning models to identify the relationship between whale occurrence and environment, and predict foraging and migration habitat suitability in Australia and Southeast Asia.

Results
Our model predictions were validated by producing spatially varying accuracy metrics. We identified the shelf off the Bonney Coast, Great Australian Bight, and southern Western Australia as well as the slope off the Western Australian coast as suitable habitat for migration, with predicted foraging/reproduction suitable habitat in Southeast Asia region occurring on slope and in deep ocean waters. Suitable foraging habitat occurred primarily on slope and shelf break throughout most of Australia, with use of the continental shelf also occurring, predominanly in South West and Southern Australia. Depth of the water column (bathymetry) was consistently a top predictor of suitable habitat for most regions, however, dynamic environmental variables (sea surface temperature, surface height anomaly) influenced the probability of whale occurrence.

Conclusions
Our results indicate suitable habitat is related to dynamic, localised oceanic processes that may occur at fine temporal scales or seasonally. An increase in the sample size of tagged whales is required to move towards developing more dynamic distribution models at seasonal and monthly temporal scales. Our validation metrics also indicated areas where further data collection is needed to improve model accuracy. This is of particular importance for pygmy blue whale management, since threats (e.g., shipping, underwater noise and artificial structures) from the offshore energy and shipping industries will persist or may increase with the onset of an offshore renewable energy sector in Australia.
Toward an Oceanic “Becoming With”
Authors: Parkin Justine 
Published: June 2024

Abstract
It is hard not to consider sea-level rise as reflective of an imminent crisis. Drawing together insights from Black feminism and Indigenous thought from the Island Pacific, this article counters a discourse of crisis without uncritically accepting the “oceanifying” of the planet. It moves between analyses of poetic, visual, and plant cultivation practices to show that there is a rubric for living and thinking with the ocean that emphasizes how human and nonhuman life can “become-with” each other in response to ecological degradation while still grappling with the lived legacies of colonial pasts. These oceanic coordinations are ways of knowing that destabilize human exceptionalism from the outset while not excluding the human entirely. Rather, these frameworks incorporate human-nonhuman relations to expand our frame of reference, challenging dominant anthropocentric narratives of crisis and helping imagine and live within oceanic futures.
A β-catenin chromobody-based probe highlights endothelial maturation during vascular morphogenesis in vivo
Authors: Gauvrit Sébastien , Zhao Shengnan , Rothbauer Ulrich , Stainier Didier Y. R. 
Published: June 2024

ABSTRACT
Visualization of protein dynamics is a crucial step in understanding cellular processes. Chromobodies, fluorescently labelled single-domain antibodies, have emerged as versatile probes for live cell imaging of endogenous proteins. However, how these chromobodies behave in vivo and how accurately they monitor tissue changes remain poorly explored. Here, we generated an endothelial-specific β-catenin chromobody-derived probe and analyzed its expression pattern during cardiovascular development in zebrafish. Using high-resolution confocal imaging, we show that the chromobody signal correlates with the localization of β-catenin in the nucleus and at cell-cell junctions, and thereby can be used to assess endothelial maturation. Loss of Cadherin 5 strongly affects the localization of the chromobody at the cell membrane, confirming the cadherin-based adherens junction role of β-catenin. Furthermore, using a genetic model to block blood flow, we observed that cell junctions are compromised in most endothelial cells but not in the endocardium, highlighting the heterogeneous response of the endothelium to the lack of blood flow. Overall, our data further expand the use of chromobodies for in vivo applications and illustrate their potential to monitor tissue morphogenesis at high resolution.
Die vier Phasen von Citizen Development-Initiativen: Treiber, Herausforderungen und Handlungsempfehlungen
Authors: Binzer Björn , Winkler Till J. 
Published: June 2024

ZusammenfassungModerne Low-Code/No-Code-Plattformen transformieren die Art und Weise, wie Unternehmen digitale Lösungen entwickeln und Innovationen voranbringen. Durch den Fortschritt in Technologien wie Cloud-Computing, künstlicher Intelligenz und maschinellem Lernen ermöglichen diese Plattformen Fachbereichsmitarbeitern ohne umfassende IT-Kenntnisse – den sogenannten Citizen Developern – das Entwerfen und Implementieren eigener leichtgewichtiger digitaler Lösungen. Dieser Trend motiviert immer mehr Unternehmen, Citizen Development-Initiativen ins Leben zu rufen und somit einen kulturellen Wandel hin zu einer demokratisierten Entwicklung digitaler Innovationen zu fördern. Trotz des erkennbaren Potenzials stehen viele Unternehmen jedoch vor der Frage, wie sie ihre Citizen Development-Initiativen effektiv starten und unternehmensweit skalieren können. Dieser Artikel beleuchtet diese Fragestellung basierend auf Interviews mit 21 Verantwortlichen für Citizen Development aus 16 verschiedenen Unternehmen, die früh in Low-Code/No-Code investiert haben. Wir identifizieren vier Phasen, die Unternehmen im Rahmen von Citizen Development durchlaufen, und die spezifischen Herausforderungen, die in jeder Phase bewältigt werden müssen. Unsere Forschungsergebnisse eröffnen Unternehmen Einblicke in aktuelle Praktiken und Zielzustände, um die Herausforderungen einer erfolgreichen Implementierung von Citizen Development zu meistern. Unternehmen sollen befähigt werden, ihre Ressourcen effizienter zu nutzen und Citizen Development nahtlos in ihre Digitalstrategie zu integrieren.
Development of an epilepsy self-management mobile health app framework: Content validity study results
Authors: Alzamanan Mohsen Zaied , Lim Kheng-Seang , Ismail Maizatul Akmar , Ghani Norjihan Abdul 
Published: June 2024

Background
Mobile health (mHealth) applications (apps) show promise in supporting epilepsy self-management (eSM). To delve deeper into this potential, we conducted a systematic review of epilepsy mHealth apps available on both iOS and Android platforms, examining articles related to eSM. This review allowed us to identify important domains related to eSM. Furthermore, based on the findings, we developed an epilepsy mHealth app framework that aims to improve self-management for the local population. This study aims to assess the practicality and usability of the proposed mHealth app framework designed to improve eSM. We will conduct an expert panel review to evaluate the effectiveness and feasibility of the framework.

Material and methods
Content validity was assessed by an expert panel comprising epileptologists and pharmacists. The validation process involved scoring the items within each domain of the framework to evaluate their practicality and usability (quantitative component). In addition, a panel discussion was conducted to further explore and discuss the qualitative aspects of the items.

Results
A total of 4 domains with 15 items were highly rated for their practicality and usefulness in eSM.

Conclusions
The locally validated framework will be useful for developing eSM mobile apps. Seizure Tracking, Medication Adherence, Treatment Management, and Healthcare Communication emerged as the most crucial domains for enhancing eSM.
AVRUPA BİRLİĞİ ADAY ÜLKELERİNE YAPILAN DOĞRUDAN YABANCI YATIRIMLARI ÜZERİNE TAHMİN TEKNİKLERİ KARŞILAŞTIRMASI
Authors: İnel Mehmet 
Published: June 2024

Globalleşen dünyada ülkelere yapılan doğrudan yatırımların önemi her geçen	gün artmaktadır. Özellikle Avrupa Birliği aday ülkelerinin doğrudan yabancı	yatırımlarının geleceğini belirlemek amacıyla tahmin tekniklerinin kullanımı	gerekmektedir. Düşük sayıda örnek büyüklüğüne sahip durumda tahmin	tekniklerini karşılaştırmayı amaçlayan, en uygun tahmin tekniğinin ne olduğu	konusunda bir incelemenin yapılacağı bu çalışmada her bir aday ülkelerin	yabancı yatırım değerleri tahmin edilecektir. Mevcut durumda uygun olan	tahmin tekniği ve tahmin yöntemleri ile ilgili gelişim alanları tartışılacaktır.
Effect of ex situ, eco-friendly ZnONPs incorporating green synthesised Moringa oleifera leaf extract in enhancing biochemical and molecular aspects of Vicia faba L. under salt stress
Authors: Alhudhaibi Abdulrahman M. , Ragab Sherif M. , Sharaf Mohamed , Turoop Losenge , Runo Steven , Nyanjom Steven , Haouala Faouzi , Hossain ABM Sharif , Alharbi Basmah M. , Elkelish Amr 
Published: June 2024

Abstract
Nevertheless, the growing salt stress in arable areas poses a significant threat to agricultural production. The current study utilised zinc oxide nanoparticles (ZnONPs) using an extract obtained from the leaves of Moringa oleifera (MOLe). The purpose was to reduce the detrimental effect of salinity on the Vicia faba L. (VF) cultivar (Nubaria 3). The biochemical and molecular characteristics of plants grown in saline settings were assessed 60 days after planting. The expression levels of plant defence genes polyphenol oxidase (PPO) and peroxidase (POX) in VF leaves were analysed at different time points. The synthesised NPs improve osmotic protection in plants under salt stress by elevated levels of pigments involved in photosynthesis. In addition, there was a significant increase in the levels of biochemical parameters, such as total phenol, proline, and antioxidant enzyme activities. In addition, the use of MOLe and MOLe@ZnONPs on the plants resulted in an increase in ion content and a considerable rise in the expression of Vf POX and Vf PPO genes when exposed to salt conditions. The findings suggest that the use of MOLe and MOLe@ZnONPs was more efficient in aiding the plant to recover from the negative impact of salt stress.
Correlation between fat-soluble vitamin levels and inflammatory factors in paediatric community-acquired pneumonia: A prospective study
Authors: Liao Jianyuan , Zhang Lifang , Chen Gangxin , Luo Yuxing 
Published: June 2024

Abstract
Community-acquired pneumonia (CAP) is a common respiratory disease in children. This prospective cohort study of 110 children with CAP and 100 healthy children investigated the relationship between the levels of vitamin A, D and E and inflammatory markers, such as tumour necrosis factor (TNF-a), interleukin-1 (IL-1), interleukin-10 (IL-10), neutrophils (NE) and C-reactive protein (CRP), in CAP. The haemoglobin, leukocyte concentration, NE, monocytes and CRP concentration in the CAP group showed significant differences (P < 0.05). The levels of vitamin A, D and E in the CAP group were lower than those in the control group, while the levels of TNF-a and IL-1 were higher than in the control group; the differences were statistically significant (P < 0.05). The IL-10 levels showed no significant differences (P > 0.05). Pearson analysis revealed that the vitamin A, D and E levels were all correlated with the TNF-a, IL-10 and CRP levels (P < 0.05). The vitamin A, D and E levels of the CAP children were lower than those of the healthy children. Thus, the content of fat-soluble vitamins is correlated with the secretion of TNF-a and IL-10. The research provides a new direction for the prevention, diagnosis and treatment of CAP.