bluuebunny/crossref_metadata_2025_split huggingface dataset:
The P2X7‐Egr pathway regulates nucleotide‐dependent inflammatory gene expression in microglia
Authors: Friedle S. A. , Brautigam V. M. , Nikodemova M. , Wright M. L. , Watters J. J. 
Published: September 2010

AbstractMicroglial hyperactivity contributes to neuronal damage resulting from CNS injury and disease. Therefore, a better understanding of endogenous microglial receptor systems that can be exploited to modulate their inflammatory functions is important if better, neuroprotective therapeutics are to be designed. Previous studies from our lab and others have demonstrated that the P2X7 purinergic receptor agonist BzATP attenuates microglial inflammatory mediator production stimulated by lipopolysaccharide (LPS), suggesting that purinergic receptors may be one such receptor system that can be used for manipulating microglial activation. However, although P2X7 receptor activation is well recognized to regulate processing and release of cytokines, little is known concerning its role in regulating the transcription of inflammatory genes, nor the molecular mechanisms underlying these transcriptional effects. In the present studies, we identify that the transcription factors early growth response (Egr)−1, −2 and −3 are downstream signaling targets of P2X7 receptors in microglia, and that their activation is sensitive to MEK and p38 mitogen‐activated protein kinase (MAPK) inhibitors. Moreover, using RNAi, we demonstrate that Egr factors and P2X7 receptors are necessary for BzATP‐mediated attenuation of iNOS, and stimulation of TNF‐α and IL‐6 gene expression. BzATP also attenuates neuronal death induced by LPS conditioned medium, and P2X7 receptors are required for this effect. These studies are the first to identify Egr factors as regulators of inflammatory gene expression following P2X7 receptor activation, and suggest that P2X7 receptors may utilize the MAPK‐Egr pathway to exert differential effects on microglial inflammatory activities which are beneficial to neuron survival. © 2010 Wiley‐Liss, Inc.
Augmenting antidepressant medication with modular CBT for geriatric generalized anxiety disorder: a pilot study
Authors: Wetherell Julie Loebach , Stoddard Jill A. , White Kamila S. , Kornblith Sander , Nguyen Hoang , Andreescu Carmen , Zisook Sidney , Lenze Eric J. 
Published: September 2010

AbstractObjectiveGeneralized anxiety disorder (GAD) is a prevalent psychiatric condition in older adults with deleterious effects on health and cognition. Although selective serotonin reuptake inhibitor (SSRI) medications have some efficacy as acute treatments for geriatric GAD, incomplete response is the most common outcome of monotherapy. We therefore developed a novel sequential treatment strategy, using personalized, modular cognitive‐behavioral therapy (mCBT) to augment SSRI medication.MethodIn an open label pilot study (N = 10), subjects received a sequenced trial of 12 weeks of escitalopram followed by 16 weeks of escitalopram augmented with mCBT. We also examined the maintenance effects of mCBT over a 28‐week follow‐up period following drug discontinuation and termination of psychotherapy.ResultsResults suggest that (1) adding mCBT to escitalopram significantly reduced anxiety symptoms and pathological worry, resulting in full remission for most patients and (2) some patients maintained response after all treatments were withdrawn.ConclusionFindings suggest that mCBT may be an effective augmentation strategy when added to SSRI medication and provide limited support for the long‐term benefit of mCBT after discontinuation of pharmacotherapy. Copyright © 2010 John Wiley & Sons, Ltd.
Presence and quality of kindergarten children's friendships: concurrent and longitudinal associations with child adjustment in the early school years
Authors: Engle Jennifer M. , McElwain Nancy L. , Lasky Nicole 
Published: September 2010

AbstractThe presence and quality of friendships are posited to have developmental significance, yet little is known about the extent to which children without friends versus low‐quality friendships compare on socioemotional adjustment. The current study utilized data from a subsample of 567 children (289 boys) participating in the NICHD Study of Early Child Care and Youth Development. Based on maternal reports at kindergarten, four friendship groups were formed: no friends, low quality, average quality, and high quality, and these groups were used to predict teacher‐reported behaviour problems and social skills concurrently (in kindergarten) and longitudinally (in first and third grade). Concurrently, low‐quality friendships were associated with greater externalizing behaviour, whereas high‐quality friendships were associated with greater social skills. Longitudinally, having no friends in kindergarten was associated with higher levels of externalizing behaviour for boys, but lower levels for girls. Children without friends also showed more internalizing problems at first grade. Lastly, having a high‐quality friendship in kindergarten was associated with greater social skills in first and third grades, but only for boys. Results underscore high‐quality friendship as a context for the development of social skills and indicate different trajectories of problem behaviour for kindergarten children with no friends versus low‐quality friendships. Copyright © 2010 John Wiley & Sons, Ltd.
Combining random forest and copula functions: A heuristic approach for selecting assets from a financial crisis perspective
Authors: De Luca Giovanni , Rivieccio Giorgia , Zuccolotto Paola 
Published: September 2010

AbstractIn this paper we propose a heuristic strategy aimed at selecting and analysing a set of financial assets, focusing attention on their multivariate tail dependence structure. The selection, obtained through an algorithmic procedure based on data mining tools, assumes the existence of a reference asset we are specifically interested to. The procedure allows one to opt for two alternatives: to prefer those assets exhibiting either a minimum lower tail dependence or a maximum upper tail dependence. The former could be a recommendable opportunity in a financial crisis period. For the selected assets, the tail dependence coefficients are estimated by means of a proper multivariate copula function. Copyright © 2010 John Wiley & Sons, Ltd.
Active shape modeling of the hip in the prediction of incident hip fracture
Authors: Baker-LePain Julie C , Luker Kali R , Lynch John A , Parimi Neeta , Nevitt Michael C , Lane Nancy E 
Published: September 2010

Abstract
The objective of this study was to evaluate right proximal femur shape as a risk factor for incident hip fracture using active shape modeling (ASM). A nested case-control study of white women 65 years of age and older enrolled in the Study of Osteoporotic Fractures (SOF) was performed. Subjects (n = 168) were randomly selected from study participants who experienced hip fracture during the follow-up period (mean 8.3 years). Controls (n = 231) had no fracture during follow-up. Subjects with baseline radiographic hip osteoarthritis were excluded. ASM of digitized right hip radiographs generated 10 independent modes of variation in proximal femur shape that together accounted for 95% of the variance in proximal femur shape. The association of ASM modes with incident hip fracture was analyzed by logistic regression. Together, the 10 ASM modes demonstrated good discrimination of incident hip fracture. In models controlling for age and body mass index (BMI), the area under receiver operating characteristic (AUROC) curve for hip shape was 0.813, 95% confidence interval (CI) 0.771–0.854 compared with models containing femoral neck bone mineral density (AUROC = 0.675, 95% CI 0.620–0.730), intertrochanteric bone mineral density (AUROC = 0.645, 95% CI 0.589–0.701), femoral neck length (AUROC = 0.631, 95% CI 0.573–0.690), or femoral neck width (AUROC = 0.633, 95% CI 0.574–0.691). The accuracy of fracture discrimination was improved by combining ASM modes with femoral neck bone mineral density (AUROC = 0.835, 95% CI 0.795–0.875) or with intertrochanteric bone mineral density (AUROC = 0.834, 95% CI 0.794–0.875). Hips with positive standard deviations of ASM mode 4 had the highest risk of incident hip fracture (odds ratio = 2.48, 95% CI 1.68–3.31, p &lt; .001). We conclude that variations in the relative size of the femoral head and neck are important determinants of incident hip fracture. The addition of hip shape to fracture-prediction tools may improve the risk assessment for osteoporotic hip fractures. © 2011 American Society for Bone and Mineral Research.
Influence of long-term postmenopausal hormone-replacement therapy on estimated structural bone strength: A study in discordant monozygotic twins
Authors: Mikkola Tuija M , Heinonen Ari , Kovanen Vuokko , Cheng Sulin , Kujala Urho M , Suominen Harri , Alén Markku , Puolakka Jukka , Ankarberg-Lindgren Carina , Ronkainen Paula HA , Koskenvuo Markku , Kaprio Jaakko , Rantanen Taina , Sipilä Sarianna 
Published: September 2010

Abstract
Although postmenopausal hormone-replacement therapy (HRT) is known to prevent fractures, knowledge on the influence of long-term HRT on bone strength and its determinants other than areal bone mineral density is scarce. This study used a genetically controlled design with 24 monozygotic female twin pairs aged 54 to 72 years in which one cotwin was using HRT (mean duration 8 years) and the other had never used HRT. Estimated bone strength, cross-sectional area, volumetric bone mineral density, bone mineral mass, and cross-sectional density and mass distributions were assessed in the tibial shaft, distal tibia, and distal radius with peripheral computed tomography (pQCT). In the tibial shaft, HRT users had 9% [95% confidence interval (CI) 3%–15%] higher estimated bending strength than their nonusing cotwins. Larger cortical area and higher cortical bone mineral density accounted for this difference. The cortex was larger in the HRT users in the endocortical region. In the distal tibia, estimated compressive strength was 24% (95% CI 9%–40%) higher and in the distal radius 26% (95% CI 11%–41%) higher in the HRT users than in their nonusing cotwins owing to higher volumetric bone mineral density. No difference between users and nonusers was observed in total bone cross-sectional area in any measured bone site. The added mineral mass in the HRT users was distributed evenly within and between bone sites. In postmenopausal women, long-term HRT preserves estimated bone strength systemically by preventing bone mineral loss similarly in body weight–loaded and non-weight-loaded bone. © 2011 American Society for Bone and Mineral Research.
Association of menopausal vasomotor symptoms with increased bone turnover during the menopausal transition
Authors: Crandall Carolyn J , Tseng Chi-Hong , Crawford Sybil L , Thurston Rebecca C , Gold Ellen B , Johnston Janet M , Greendale Gail A 
Published: September 2010

Abstract
The purpose of this study was to determine the longitudinal association between menopausal vasomotor symptoms (VMS) and urinary N-telopeptide level (NTX) according to menopausal stage. We analyzed data from 2283 participants of the Study of Women's Health Across the Nation, a longitudinal community-based cohort study of women aged 42 to 52 years at baseline. At baseline and annually through follow-up visit 8, participants provided questionnaire data, urine samples, serum samples, and anthropometric measurements. Using multivariable repeated-measures mixed models, we examined associations between annually assessed VMS frequency and annual NTX measurements. Our results show that mean adjusted NTX was 1.94 nM of bone collagen equivalents (BCE)/mM of creatinine higher among early perimenopausal women with any VMS than among early perimenopausal women with no VMS (p &lt; .0001). Mean adjusted NTX was 2.44 nM BCE/mM of creatinine higher among late perimenopausal women with any VMS than among late perimenopausal women with no VMS (p = .03). Among premenopausal women, VMS frequency was not significantly associated with NTX level. When NTX values among women with frequent VMS (≥6 days in past 2 weeks) were expressed as percentages of NTX values among women without frequent VMS, the differences were 3% for premenopausal women, 9% for early perimenopausal women, 7% for late perimenopausal women, and 4% for postmenopausal women. Adjustment for serum follicle-stimulating hormone (FSH) level greatly reduced the magnitudes of associations between VMS and NTX level. We conclude that among early perimenopausal and late perimenopausal women, those with VMS had higher bone turnover than those without VMS. Prior to the final menstrual period, VMS may be a marker for risk of adverse bone health. © 2011 American Society for Bone and Mineral Research.
Conserved regulatory motifs in osteogenic gene promoters integrate cooperative effects of canonical Wnt and BMP pathways
Authors: Rodríguez-Carballo Edgardo , Ulsamer Arnau , Susperregui Antonio RG , Manzanares-Céspedes Cristina , Sánchez-García Eva , Bartrons Ramon , Rosa José Luis , Ventura Francesc 
Published: September 2010

Abstract
Osteoblast differentiation depends on the coordinated network of evolutionary conserved transcription factors during bone formation and homeostasis. Evidence indicates that bone morphogenetic protein (BMP) and Wnt proteins regulate several steps of skeletal development. Here, we provide a molecular description of the cooperative effects of BMP and Wnt canonical pathway on the expression of the early osteogenic genes Dlx5, Msx2, and Runx2 in C2C12 cells, primary cultures of bone marrow–mesenchymal stem cells, and organotypic calvarial cultures. Coordinated regulation of these genes leads to the cooperative activation of their downstream osteogenic target gene osterix. Induction of these genes is mediated through enhancer regions with an evolutionary conserved structure encompassing both Smad and TCF/LEF1 DNA-binding sites. Formation of a cooperative complex is mediated through DNA binding of Smads and TCF4/β-catenin to their cognate sequences, as well as protein-protein interactions between them. The formation of these cooperative transcriptional complexes results in a more efficient recruitment of coactivators such as p300. We propose that evolutionary conserved regulatory regions in specific osteogenic master genes are key integrative modules during osteogenesis. © 2011 American Society for Bone and Mineral Research.
A methodology for Raman structural quantification imaging and its application to iron indoor atmospheric corrosion products
Authors: Monnier Judith , Bellot‐Gurlet Ludovic , Baron Denis , Neff Delphine , Guillot Ivan , Dillmann Philippe 
Published: September 2010

AbstractRaman structural imaging can extract original information in order to answer new questions raised in the recent studies on iron and low alloy steel corrosion. Up to now, this technique has allowed the extraction of the qualitative distribution of the compounds constituting the corrosion product layers. We propose here a methodology to extract quantitative parameters from Raman hyperspectral maps, executed by a home‐developed software: LADIR‐CAT. Specifically developed for iron corrosion quantitative component imaging, the approach and program operation are described. The LADIR‐CAT is applied on ancient corroded iron samples originating from the Amiens cathedral (France) to establish the description and the composition of the corrosion system through quantitative compound imaging. Copyright © 2010 John Wiley & Sons, Ltd.
UV resonance Raman study of cation–π interactions in an indole crown ether
Authors: Schlamadinger Diana E. , Daschbach Megan M. , Gokel George W. , Kim Judy E. 
Published: September 2010

AbstractUV resonance Raman (UVRR) spectroscopy is used to probe changes in vibrational structure associated with cation–π interactions for the most prevalent amino acid π–donor, tryptophan. The model compound studied here is a diaza crown ether with two indole substituents. In the presence of sodium or potassium sequestered in the crown ether, or a protonated diaza group on the compound, the indole moieties participate in a cation–π interaction in which the pyrrolo group acts as the primary π‐donor. Systematic shifts in relative intensity in the 760–780 cm−1 region are observed upon formation of this cation–π interaction; we propose that these modifications reflect shifts of the delocalized, ring‐breathing W18 and hydrogen‐out‐of‐plane (HOOP) vibrational modes in this spectral region. The observed changes are attributed to perturbations of the π‐electron density as well as of normal modes that involve large displacement of the hydrogen atom on the C2 position of the pyrrole ring. Modest variations in the UVRR spectra for the three complexes studied here are correlated to differences in cation–π strength. Specifically, the UVRR spectrum of the sodium‐bound complex differs from those of the potassium‐bound or protonated‐diaza complexes, and may reflect the observation that the C2 hydrogen atom in the sodium‐bound complex exhibits the greatest perturbation relative to the other species. Normal modes sensitive to hydrogen‐bonding, such as the tryptophan W10, W9, and W8 modes, also undergo shifts in the presence of the salts. These shifts reflect the strength of interaction of the indole NH group with the iodide or hexafluorophosphate counteranion. The current observation that the W18 and HOOP normal mode regions of the indole crown ether compound are sensitive to cation–pyrrolo π interactions suggests that this region may provide reliable spectroscopic evidence of these important interactions in proteins. Copyright © 2010 John Wiley & Sons, Ltd.
Structural and nonlinear optical properties of cross‐conjugated system benzophenone thiosemicarbazone: a vibrational spectroscopic study
Authors: Ravikumar C. , Hubert Joe I. 
Published: September 2010

AbstractThe nonlinear optical (NLO) compound of interest benzophenone thiosemicarbazone (BTSC) was grown and the molecular structure generated with the aid of density functional theory (DFT). FT‐Raman and IR spectra were recorded and analyzed. The harmonic wavenumbers and IR and Raman intensities were computed with the B3LYP method. The observed vibrational wavenumbers were compared with the calculated results. The assignments of the experimental spectra were made with the help of normal coordinate analysis (NCA) following the scaled quantum mechanical force field (SQMFF) methodology. The electronic structure of the most important molecular fragments is described in terms of natural bond orbital (NBO) analysis. Copyright © 2010 John Wiley & Sons, Ltd.
Damage and molecular changes under a laser beam in SEM‐EDX/MRS interface: a case study on iron‐rich particles
Authors: Worobiec A. , Darchuk L. , Brooker A. , Potgieter H. , Van Grieken R. 
Published: September 2010

AbstractThe control of damage to individual environmental particles by a laser beam during Raman spectroscopy carried out in ambient air is generally well understood. The nature and control of damage under vacuum conditions (e.g. in the scanning electron microscopy with energy X‐ray detection combined with micro‐Raman spectroscopy—interfaced SEM‐EDX/MRS) are more complex and less well comprehended. The physical and chemical processes that affect the damage caused to small particles by lasers still remain somewhat unclear, but certainly the atmosphere (vacuum/air) and the beam intensity have very significant influences. Furthermore, it has been determined that some particles (e.g. haematite), although stable under an electron beam, are damaged by the laser beam, hampering their analysis. Additionally, when simultaneous analyses by SEM/EDX and MRS are considered, the correct choice of the collection surface plays a crucial role. As a result, the following collection substrates were tested to determine their influence on the laser beam damage process to the particle: silver and aluminium foils and silicon wafers. A test study was performed using artificial examples of haematite (Fe2O3) particles. Exposure of Fe2O3 particles in vacuum to 514‐ and 785‐nm laser radiation often leads to their melting, transformation and evaporation. The dependence of the damage caused by the laser beam on the particle structure is reported here. Molecular and crystallographic changes have also been revealed. Formation of magnetite (as an effect of re‐crystallisation) and Raman inactive structures was detected. Copyright © 2010 John Wiley & Sons, Ltd.
A comprehensive spectroscopic study of synthetic Fe2+, Fe3+, Mg2+ and Al3+ copiapite by Raman, XRD, LIBS, MIR and vis–NIR
Authors: Kong W. G. , Wang Alian , Freeman John J. , Sobron Pablo 
Published: September 2010

AbstractThe identification of iron sulfates on Mars by the Mars Exploration Rovers (MERs) and the Mars Reconnaissance Orbiter emphasized the importance of studying iron sulfates in laboratory simulation experiments. The copiapite group of minerals was suggested as one of the potential iron sulfates occurring on the surface and subsurface on Mars, so it is meaningful to study their spectroscopic features, especially the spectral changes caused by cation substitutions. Four copiapite samples with cation substitutions (Fe3+, Al3+, Fe2+, Mg2+) were synthesized in our laboratory. Their identities were confirmed by powder X‐ray diffraction (XRD). Spectroscopic characterizations by Raman, mid‐IR, vis‐NIR and laser‐induced‐breakdown spectroscopy (LIBS) were conducted on those synthetic copiapite samples, as these technologies are being (and will be) used in current (and future) missions to Mars. We have found a systematic ν1peak shift in the Raman spectra of the copiapite samples with cation substitutions, a consistent atomic ratio detection by LIBS, a set of systematic XRD line shifts representing structural change caused by the cation substitutions and a weakening of selection rules in mid‐IR spectra caused by the low site symmetry of (SO4)2− in the copiapite structures. The near‐infrared (NIR) spectra of the trivalent copiapite species show two strong diagnostic water features near 1.4 and 1.9 µm, with two additional bands near 2.0 µm. In the vis‐NIR spectra, the position of an electronic band shifts from 0.85 µm for ferricopiapite to 0.866 µm for copiapite, and this shift suggests the appearance of a Fe2+ electronic transition band near 0.9 µm. Copyright © 2010 John Wiley & Sons, Ltd.
Raman study of NiFe2O4 nanoparticles, bulk and films: effect of laser power
Authors: Ahlawat Anju , Sathe V. G. 
Published: September 2010

AbstractNanoparticles, bulk and thin films of NiFe2 O4 compounds are studied by micro‐Raman spectroscopy. The effect of varying the incident laser power up to 40 mW was studied in all forms of the samples. The spectra showed a large magnitude of red shift and line broadening as a result of high incident laser power. It is shown that the inverse spinel structure remains robust, and no trace of laser‐induced oxidation was observed. The low‐temperature study of the bulk and nanoparticles has also been carried out for elucidating thermal effects due to the high incident laser power. The rise in temperature for maximum incident laser power of 40 mW was estimated to be ∼625 °C. Copyright © 2010 John Wiley & Sons, Ltd.