bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vilssteg als Wahrzeichen des Mäandertals
Authors: Bauer Martin 
Published: December 2009

AbstractIm Zuge einer städtebaulichen Maßnahme im niederbayerischen Vilstal wurde zur Aufwertung des Flussgebietes eine Fußgängerbrücke errichtet. Mit einer filigranen abgespannten Konstruktion gelang ein Bauwerk, das sich sowohl landschaftlich in das Gelände einfügt als auch den Erschließungsgedanken von Schule und Sportgelände erfüllt. Im nachfolgenden Beitrag werden die Geschichte des Vilsstegs sowie dessen Konstruktion und Bau näher erläutert.Vilssteg – a pedestrian bridge as a landmark for the Bavarian Mäandertal.In the course of an urban development in the Lower Bavarian Vilstal a pedestrian bridge has been erected to upvalue the surroundings of the river. With a filigree guyed construction, a building structure has been succeeded which fits scenically into the terrain as well as it fulfils the development idea of school and sports ground. The following paper comments on the history of the Vilssteg and its construction.
Hangar für drei Großraumflugzeuge
Authors: Kraus Matthias , Niebuhr Hans Joachim 
Published: December 2009

AbstractAm Flughafen Düsseldorf International ist ein neuer Hangar, die Halle 7, gebaut worden. Es handelt sich hierbei um das größte Bauprojekt der Flughafengesellschaft seit der Terminalerweiterung in den Jahren von 1998 bis 2004. Mit Abmessungen von ca. 210 m x 90 m ist die Flugzeughalle die größte am Düsseldorf Airport beziehungsweise in Nordrhein‐Westfalen. Sie bietet zeitgleich Platz für drei Großraumflugzeuge. Im Wesentlichen ist das Haupttragwerk als reine Stahlkonstruktion und bei den Stützen auch in Verbundbauweise ausgeführt worden. Neben der Flugzeughalle sind ein zweigeschossiger Anbau mit Lager‐, Aufenthalts‐ und Sozialräumen sowie ein viergeschossiger Bürotrakt mit einem Technikbereich im Keller in Massivbauweise entstanden. Im Folgenden wird die Konstruktion des Hangars vorgestellt, die Anbauten in Massivbauweise sind nicht Bestandteil dieses Beitrages.Hangar for three wide‐bodied aircrafts.At Düsseldorf International Airport a new Hangar referred to as Hall 7 has been built. It is the biggest project of the Airport Corporation since the extension of the terminal in the years 1998‐2004. With dimensions of about 210 m width and 90 m depth, the aero plane hangar is the largest one at Düsseldorf Airport and North Rhine‐Westphalia. The building has a maintenance capacity of three wide‐bodied long‐haul aircrafts. The structure is mainly designed as steel construction, although some of the columns are designed as composite members as well. Adjacently behind the hangar a two storey outbuilding with stocking and social rooms and a four storey office building with technical equipment in the basement were built as massive concrete construction. In the following the hangar will be presented, the additional outbuildings are not included in this essay.
A Unique Interplay Between Rap1 and E-Cadherin in the Endocytic Pathway Regulates Self-Renewal of Human Embryonic Stem Cells
Authors: Li Li , Wang Shuai , Jezierski Anna , Moalim-Nour Lilian , Mohib Kanishka , Parks Robin J. , Francesco Retta Saverio , Wang Lisheng 
Published: December 2009

Abstract
Regulatory mechanisms pertaining to the self-renewal of stem cells remain incompletely understood. Here, we show that functional interactions between small GTPase Rap1 and the adhesion molecule E-cadherin uniquely regulate the self-renewal of human embryonic stem cells (hESCs). Inhibition of Rap1 suppresses colony formation and self-renewal of hESCs, whereas overexpression of Rap1 augments hESC clonogenicity. Rap1 does not directly influence the expression of the pluripotency genes Oct4 and Nanog. Instead, it affects the endocytic recycling pathway involved in the formation and maintenance of E-cadherin-mediated cell–cell cohesion, which is essential for the colony formation and self-renewal of hESCs. Conversely, distinct from epithelial cells, disruption of E-cadherin mediated cell–cell adhesions induces lysosome delivery and degradation of Rap1. This in turn leads to a further downregulation of E-cadherin function and a subsequent reduction in hESC clonogenic capacity. These findings provide the first demonstration that the interplay between Rap1 and E-cadherin along the endocytic recycling pathway serves as a timely and efficient mechanism to regulate hESC self-renewal. Given the availability of specific activators for Rap1, this work provides a new perspective to enable better maintenance of human pluripotent stem cells.
Boundary Cap Cells are Highly Competitive for CNS Remyelination: Fast Migration and Efficient Differentiation in PNS and CNS Myelin-Forming Cells
Authors: Zujovic V. , Thibaud J. , Bachelin C. , Vidal M. , Coulpier F. , Charnay P. , Topilko P. , Evercooren A. Baron-Van 
Published: December 2009

Abstract
During development, boundary cap cells (BC) and neural crest cell (NCC) derivatives generate Schwann cells (SC) of the spinal roots and a subpopulation of neurons and satellite cells in the dorsal root ganglia. Despite their stem-like properties, their therapeutic potential in the diseased central nervous system (CNS) was never explored. The aim of this work was to explore BC therapeutic potential for CNS remyelination. We derived BC from Krox20Cre × R26RYfp embryos at E12.5, when Krox20 is exclusively expressed by BC. Combining microdissection and cell fate mapping, we show that acutely isolated BC are a unique population closely related but distinct from NCC and SC precursors. Moreover, when grafted in the demyelinated spinal cord, BC progeny expands in the lesion through a combination of time-regulated processes including proliferation and differentiation. Furthermore, when grafted away from the lesion, BC progeny, in contrast to committed SC, show a high migratory potential mediated through enhanced interactions with astrocytes and white matter, and possibly with polysialylated neural cell adhesion molecule expression. In response to demyelinated axons of the CNS, BC progeny generates essentially myelin-forming SC. However, in contact with axons and astrocytes, some of them generate also myelin-forming oligodendrocytes. There are two primary outcomes of this study. First, the high motility of BC and their progeny, in addition to their capacity to remyelinate CNS axons, supports the view that BC are a reservoir of interest to promote CNS remyelination. Second, from a developmental point of view, BC behavior in the demyelinated CNS raises the question of the boundary between central and peripheral myelinating cells.
High-Throughput Flow Cytometry Purification of Transduced Progenitors Expressing Defined Levels of Vascular Endothelial Growth Factor Induces Controlled Angiogenesis In Vivo
Authors: Misteli Heidi , Wolff Thomas , Füglistaler Philipp , Gianni-Barrera Roberto , Gürke Lorenz , Heberer Michael , Banfi Andrea 
Published: December 2009

Abstract
Delivery of therapeutic genes by genetically modified progenitors is a powerful tool for regenerative medicine. However, many proteins remain localized within or around the expressing cell, and heterogeneous expression levels can lead to reduced efficacy or increased toxicity. For example, the matrix-binding vascular endothelial growth factor (VEGF) can induce normal, stable, and functional angiogenesis or aberrant angioma growth depending on its level of expression in the microenvironment around each producing cell, and not on its total dose. To overcome this limitation, we developed a flow cytometry–based method to rapidly purify transduced cells expressing desired levels of a therapeutic transgene. Primary mouse myoblasts were transduced with a bicistronic retrovirus expressing VEGF linked to a nonfunctional, truncated form of the syngenic molecule CD8a. By using a clonal population uniformly expressing a known VEGF level as a reference, cells producing similar VEGF amounts were rapidly sorted from the primary population on the basis of their CD8a fluorescence intensity. A single round of sorting with a suitably designed gate yielded a purified population that induced robust, normal, and stable angiogenesis, and completely avoided angioma growth, which was instead always caused by the heterogeneous parent population. This clinically applicable high-throughput technique allowed the delivery of highly controlled VEGF levels in vivo, leading to significantly improved safety without compromising efficacy. Furthermore, when applied to other suitable progenitor populations, this technique could help overcome a significant obstacle in the development of safe and efficacious vascularization strategies in the fields of regenerative medicine and tissue engineering.
Water‐forming NADH oxidase protects Torulopsis glabrata against hyperosmotic stress
Authors: Xu Sha , Zhou Jingwen , Qin Yi , Liu Liming , Chen Jian 
Published: December 2009

AbstractA heterologous water‐forming NADH oxidase was introduced into Torulopsis glabrata and the effect on cell growth under hyperosmotic conditions was investigated. Expression of the noxE gene from Lactococcus lactis NZ9000 in T. glabrata resulted in a marked decrease in the NADH : NAD+ ratio and higher activities of key enzymes in water‐regenerating pathways, leading to an increase in intracellular water content. NaCl‐induced reactive oxygen species production was also decreased by the introduction of NADH oxidase, resulting in a significant increase in the growth of T. glabrata under hyperosmotic stress conditions (3824 mOsmol/kg). The results indicated that the osmotolerance of cells can be enhanced by manipulating water‐production pathways. Copyright © 2009 John Wiley & Sons, Ltd.
Expression of Paracoccidioides brasiliensis CHS3 in a Saccharomyces cerevisiae chs3 null mutant demonstrates its functionality as a chitin synthase gene
Authors: Barreto Laura , Sorais Françoise , Salazar Victor , San‐Blas Gioconda , Niño‐Vega Gustavo A. 
Published: December 2009

AbstractWe report the isolation and sequencing of CHS3, a gene that encodes one of several chitin synthases in Paracoccidioides brasiliensis, a medically important fungus restricted geographically to Latin America. The gene contains a single open reading frame of 3817 bp with two introns (71 and 86 bp) and encodes a 1220 amino acid polypeptide with high similarity to other fungal chitin synthases. Northern analysis reveals a high expression of CHS3 in the pathogenic yeast‐like phase of the fungus and at the end of the mycelium–yeast transition. Expression of P. brasiliensis CHS3 in a Saccharomyces cerevisiae chs3 null mutant enhanced calcofluor white staining in parallel to an increase in total chitin synthase activity and chitin content in its cell wall. The sequence of P. brasiliensis CHS3 is available at the GenBank database under Accession No. AF107623.2. Copyright © 2009 John Wiley & Sons, Ltd.
Addressing the Global Burden of Surgical Disease: Proceedings from the 2nd Annual Symposium at the American College of Surgeons
Authors: Perkins R. Serene , Casey Kathleen M. , McQueen K. A. Kelly 
Published: December 2009

AbstractIn the past decade, interest in surgery as a means to improve public health and engage in international service has increased significantly. International organizations, academic institutions, professional associations, and humanitarian aid organizations recognize that disparate access to surgical care affects global health and they have recently joined forces to address access to surgical care. Current initiatives focus on quantitatively defining surgical disparity, prioritizing a surgical agenda, and developing economically sustainable models for health care assistance, training, and delivery. The Global Burden of Surgical Disease Working Group (GBoSD WG) strives to (1) quantitatively define global disparity in surgical care; (2) assess unmet surgical need; (3) identify priorities; (4) develop sustainable models for improved health care delivery; and (5) advocate for a surgical presence within the global public health agenda. This article formally introduces the GBoSD Working Group and papers presented during the 2009 Symposium at the American College of Surgeons in Chicago.
Pain as the Only Consistent Sign of Acute Appendicitis: Lack of Inflammatory Signs Does Not Exclude the Diagnosis
Authors: Monneuse Olivier , Abdalla S. , Pilleul F. , Hervieu V. , Gruner L. , Tissot E. , Barth X. 
Published: December 2009

AbstractBackgroundThe clinical diagnosis of acute appendicitis in adults remains tricky, but radiological examinations are very helpful to determine the diagnosis even when the adult patient presents atypically. This study was designed to quantify the proportion of patients with a preoperative diagnosis of acute appendicitis that had isolated right lower quadrant pain without biological inflammatory signs and then to determine which imaging examination led to the determination of the diagnosis.MethodsIn this monocentric study based on retrospectively collected data, we analyzed a series of 326 patients with a preoperative diagnosis of acute appendicitis and isolated those who were afebrile and had isolated right lower quadrant pain and normal white blood cell counts and C‐reactive protein levels. We determined whether the systematic ultrasonography examination was informative enough or a complementary intravenous contrast media computed tomography scan was necessary to determine the diagnosis, and whether the final pathological diagnosis fit the preoperative one.ResultsA total of 15.6% of the patients with a preoperative diagnosis of acute appendicitis had isolated rebound tenderness in the right lower quadrant, i.e., they were afebrile and their white blood cell counts and C‐reactive protein levels were normal. In 96.1% of the cases, the ultrasonography examination, sometimes complemented by an intravenous contrasted computed tomography scan if the ultrasonography result was equivocal, fit the histopathological diagnosis of acute appendicitis.ConclusionsThe diagnosis of acute appendicitis cannot be excluded when an adult patient presents with isolated rebound tenderness in the right lower quadrant even without fever and biological inflammatory signs. In our study, ultrasonography and computed tomography were very helpful when making the final diagnosis.
Brief report: Emotion regulation and coping as moderators in the relationship between personality and self‐injury
Authors: Hasking Penelope A. , Coric Sarah J. , Swannell Sarah , Martin Graham , Thompson Holly Knox , Frost Aaron D.J. 
Published: December 2009

ABSTRACTSelf‐injury without conscious suicidal intent is an increasingly prevalent phenomenon particularly among adolescent populations. This pilot study examined the extent and correlates of self‐injurious behaviour in a school population sample of 393 adolescents (aged 13–18 years) using a self‐report questionnaire. Specifically, we aimed to determine whether personality was related to self‐injury and whether this relationship was moderated by emotion regulation or coping strategies. Few personality and coping variables were directly related to self‐injury after controlling for age and psychopathology. However the relationship between personality and self‐injury was moderated by coping skills and emotion regulation. We suggest future research explore these relationships in order to determine the role of coping skills and emotional regulation training in prevention of self‐injury.
Neuronal hyperactivity at the spinal cord and periaqueductal grey during painful diabetic neuropathy: Effects of gabapentin
Authors: Morgado Carla , Terra Patrícia Pereira , Tavares Isaura 
Published: December 2009

AbstractPainful diabetic neuropathy may be due to impairments in descending modulation of nociceptive transmission at the spinal cord. In the present study, streptozotocin diabetic rats (STZ rats) with neuropathic symptoms (mechanical hypersensitivity) were used to perform a time‐course evaluation of neuronal activity at the spinal dorsal horn and at the periaqueductal grey matter (PAG), a major brainstem area of pain modulation. The expression of Fos protein, a marker of nociceptive activation, progressively increased at the spinal dorsal horn at 4 and 10 weeks. At the PAG, increases in Fos expression were detected until the 4th week, with a reversal to baseline values at 10 weeks in all areas except the ventrolateral PAG. Co‐localisation of Fos with NeuN ascertained the neuronal nature of Fos‐expressing cells at the spinal cord and PAG. Four weeks after diabetes induction, the effects of gabapentin (i.p. injection of 50 mg/kg, daily during 3 days) were assessed. Gabapentin decreased Fos expression at the spinal cord and PAG and reversed mechanical hypersensitivity. The present study shows that diabetic neuropathy is accompanied by a progressive increase of the spontaneous neuronal activity at the spinal cord. Changes in descending modulation of nociceptive transmission from the PAG are likely to occur during diabetic neuropathy, probably with exacerbation of facilitatory actions. The effects of gabapentin in reversing the behavioural signs of diabetic neuropathy and neuronal hyperactivity in the spinal cord and PAG reinforce the central causes of diabetic neuropathy and point to the central targets of the drug.
Mu‐opioid receptors are not necessary for nortriptyline treatment of neuropathic allodynia
Authors: Bohren Yohann , Karavelic Dzenan , Tessier Luc‐Henri , Yalcin Ipek , Gavériaux‐Ruff Claire , Kieffer Brigitte L. , Freund‐Mercier Marie‐José , Barrot Michel 
Published: December 2009

AbstractTricyclic antidepressants (TCAs) are among the first line treatments clinically recommended against neuropathic pain. However, the mechanism by which they alleviate pain is still unclear. Pharmacological and genetic approaches evidenced a critical role of delta‐opioid receptors (DORs) in the therapeutic action of chronic TCA treatment. It is however unclear whether mu‐opioid receptors (MORs) are also necessary to the pain‐relieving action of TCAs. The lack of highly selective MOR antagonists makes difficult to conclude based on pharmacological studies. In the present work, we thus used a genetic approach and compared mutant mice lacking MORs and their wild‐type littermates. The neuropathy was induced by unilateral sciatic nerve cuffing. The threshold for mechanical response was evaluated using von Frey filaments. MOR‐deficient mice displayed the same baseline for mechanical sensitivity as their wild‐type littermates. After sciatic nerve cuffing, both wild‐type and MOR‐deficient mice displayed an ipsilateral mechanical allodynia. After about 10 days of treatment, nortriptyline suppressed this allodynia in both wild‐type and MOR‐deficient mice. MORs are thus not critical for nortriptyline action against neuropathic pain. An acute injection of the DOR antagonist naltrindole induced a relapse of neuropathic allodynia in both wild‐type and MOR‐deficient mice, thus confirming the critical role of DORs in nortriptyline action. Moreover, morphine induced an acute analgesia in control and in neuropathic wild‐type mice, but was without effect in MOR‐deficient mice. While MORs are crucial for morphine action, they are not critical for nortriptyline action. Our results highlight the functional difference between DORs and MORs in mechanisms of pain relief.
Prenatal diclofenac sodium administration increases the number of Purkinje cells in female rats: a stereological study
Authors: Odaci Ersan , Cihan Omer Faruk , Aslan Huseyin , Ragbetli Murat Cetin , Kaplan Suleyman 
Published: December 2009

AbstractDiclofenac sodium (DS) may affect the number of Purkinje cells in the developing cerebellum since DS can easily be transported from the maternal to the fetal physiological system during the pregnancy. In the present study, the effects of prenatal exposure to DS on the number of Purkinje cells in the cerebellum of 4‐week‐old (4W‐old) and 20‐week‐old (20W‐old) female rats were investigated. There were two main groups: the drug‐treated group (DTG) and the control group (CG). Beginning from the 5th day after mating for a period of 15 days, a daily dose of 1 mg/kg of DS (Voltaren, 75 mg/3 ml ampul, Novartis, Mefar Ilaç Sanayi A.S., Kartal, İstanbul, Turkey) was intraperitoneally injected in the DTG of pregnant rats. In contrast, a daily dose of 1 ml/kg of isotonic saline was intraperitoneally administered to the CG of pregnant rats during the same period. After spontaneous delivery, female offspring were obtained, and the main groups’ offspring were divided into two subgroups as a 4W‐old group and a 20W‐old group. Therefore, there were four groups at the end of the experiment: the 4W‐old DTG and the CG, and the 20W‐old DTG and the CG. At the end of 4W and 20W, offspring were perfused, their brains were dissected, and the number of cells estimated via the optical fractionator technique. Our results showed that while the total number of Purkinje cells in the cerebellum of offspring of DT 20W‐old female rats was significantly higher than that of the CG, there was no significant difference between the 4W‐old DTG and the control groups. Therefore, it could be suggested that DS administration during the prenatal period increases the number of Purkinje cells in the cerebellum of a developing female rat throughout postnatal 20W.
The 2009–2010 El Niño: Hydrologic Relief to U.S. Regions
Authors: Tootle Glenn A. , Piechota Thomas C. , Aziz Oubeidillah , Miller W. Paul , Lakshmi Venkat , Dracup John A. , Jerla Carly 
Published: December 2009

Current forecasts by the U.S. National Oceanic and Atmospheric Administration (NOAA) are that the Pacific Ocean will experience El Niño conditions in late 2009 and into 2010. These forecasts are similar to past El Ni˜o events in 1972–1973, 1982–1983, 1986–1987, and 2002–2003.Evaluating the hydrologic conditions for these past El Niño events reveals that during these times, surface water supply conditions improved in many parts of the United States, including the Southeast, Midwest, and Southwest. At the same time, the Pacific Northwest and other specific regions of the United States experienced below‐average water supply conditions. This is consistent with the long‐established linkages between oceanic‐atmospheric phenomena, El Niño, and streamflow [e.g., Kahya and Dracup, 1993; Tootle et al., 2005].
Active‐Source Seismic Experiment Confirms the Magma Pathway of Mount Asama, Japan
Authors: Aoki Yosuke , Takeo Minoru , Morita Yuichi , Tsutsui Tomoki 
Published: December 2009

Large volcanic eruptions result from the ejection of magma transported from depth. How the magma is transported to the surface is one of the fundamental questions in understanding how a volcano works. A way to address this question is to explore the seismic structures of volcanoes.One volcano that is well surveyed and instrumented through a variety of global positioning system and seismic networks is Japan's Mount Asama. Because of this, scientists were able to conduct an active‐source seismic experiment on this volcano with the goal of mapping volcanic conduits (see Figure 1).