bluuebunny/crossref_metadata_2025_split huggingface dataset:
250 Effects of premix carriers rich in iron, calcium, or fiber on growth performance of early-growing pigs
Authors: Cheng Liangkai , Liu Xule , Srisap Thirayot , Ju Tingting , Stoner Gary , Wantanasak Apichat , Guo Jiyao , Xu Xiao 
Published: May 2024

Abstract
The study was to evaluate the effect of premix carriers rich in iron (sand), calcium (limestone), or fiber (rice hull) on growth performance of early-growing pigs. A total of 215 pigs [initial body weight (BW) = 39.90 ± 5.06 kg) were randomly allotted to 3 dietary treatments containing 2% of different premix carriers: sand, limestone, or rice hull (30% crude fiber) using initial BW and sex as blocks (5 to 6 pigs per pen, 14 pens per treatment). The total Ca in sand, limestone, and rice hull were 4.28, 39.52, and 0.27 %, respectively, with Fe levels of 2.39, 0.20, and 0.05%, respectively. The analyzed total Ca level of the experimental diet containing sand, limestone, or rice hull was 0.69, 0.71, or 1.88 %, the analyzed total P level was 0.49, 0.49, or 0.48 %, and the analyzed total Fe level was 305, 633, or 347 ppm, respectively with 144 ppm of Fe from FeSO4·H2O in each diet. During the 28 d of the experiment, BW, feed disappearance, and fecal scores (ranging from 0 to 3 with 3 indicating watery feces) were recorded. Blood and feces were collected at the end of the experiment to measure the ATTD of dry matter (DM) and CP, serum iron concentration, hemoglobin, and red blood cell count. Data were analyzed by ANOVA using the Mixed Procedure of SAS with different premix carriers as the fixed effect. Limestone reduced the average daily gain (ADG; P &lt; 0.05, 0.871 vs. 0.965 and 0.935 kg/d), increased feed conversion ratio (FCR; P &lt; 0.05, 2.55 vs. 2.33 and 2.36), and aggravated diarrhea severity as reflected by greater fecal scores (P &lt; 0.05, 1.53 vs. 1.18 and 1.19) compared with sand and rice hull as carriers. The ATTD of both DM and CP in pigs fed a premix with sand as a carrier were greater than that in rice hull- or limestone-treated pigs (P &lt; 0.05, DM: 86.7 vs. 77.7 or 74.1%; CP: 86.1 vs. 75.0 or 73.3%). Furthermore, pigs fed a premix containing sand exhibited the greatest serum iron concentrations among treatment groups (sand, limestone, and rice hull: 2.81, 1.93, and 1.69 mg/L). However, no significant difference was observed in red blood cell count or hemoglobin concentrations among groups. In sum, excessive limestone in premix can negatively affect growth performance and fecal consistency, whereas using sand rich in iron as a carrier in premix exerted minimal impact on nutrient digestibility as opposed to limestone or rice hull.
247 Evaluating the use of soybean hulls on growth performance, carcass composition, and digestibility when fed to grow-finish pigs
Authors: Miller Kayla A , Mendoza Omarh F , Sparks Chris , Gabler Nicholas K 
Published: May 2024

Abstract
The addition of fiber to grow-finish diets is commonly utilized in swine production formulation as energy costs increase. The objective of this study was to determine the effects of feeding soybean hulls (SBH) at increasing levels to grow-finish pigs on growth performance, carcass characteristics, and apparent total tract digestibility (ATTD) of nutrients. Utilizing 192 grower pigs [PIC 337×1050, initial body weight (BW) = 21.99 ± 2.75 kg] that were randomly assigned to split sexed pens (n = 12 pens/treatment), pens were assigned to one of four dietary treatments and fed over three feeding phases for the estimated BW ranges of 22 to 50 kg, 50 to 86 kg, and 86 to 127 kg. Diets were formulated to represent conventional corn and soybean meal diets, containing increasing levels of SBH at: 1) 0% SBH (0%); 2) 7.5% SBH (7.5%); 3) 15% SBH (15%); and 4) 22.5% SBH (22.5%). Within phase, diets had equal SID Lys:ME and energy was allowed to float. Individual BW and pen feed disappearance were collected for each phase to calculate pen average daily gain (ADG), average daily feed intake (ADFI), and feed efficiency gain to feed ratio (G:F). On d 56 of study and during phase 2, 32 gilts (n = 8) were placed into individual metabolism crates, where total urine and feces were collected for 72 h to measure ATTD of gross energy (GE), nitrogen (N), neutral detergent fiber (NDF), and acid detergent fiber (ADF), then returned to original pens. At the completion of study, carcass weight, backfat depth, and loin depth were collected. Data were analyzed with the fixed effects of dietary treatment by ANOVA, linear, and quadratic contrasts. Pen served as the experimental unit for performance and carcass characteristics and pig as the experimental unit for ATTD analyses. Overall, as SBH increased from 0% to 22.5%, ADG tended to decrease (linear P = 0.060) with no difference in ADFI (P &gt; 0.05), resulting in an overall decrease in G:F (0.38, 0.37, 0.36, 0.35; linear P &lt; 0.001; 0, 7.5, 15 and 22.5% SBH, respectively). Final BW tended to decrease (linear P = 0.069) and carcass weight decreased (102.9, 102.7, 99.7, and 96.5 kg, linear P &lt; 0.001) as SBH increased in diet. Carcass fat depth (P = 0.014) and loin depth (P = 0.006) linearly decreased with increasing levels of SBH in the diet. Increasing levels of SBH from 0% to 22.5% decreased ATTD of GE and N (linear P &lt; 0.001) and tended to increase ATTD NDF and ADF (linear P = 0.056 and P = 0.073, respectively). Altogether, these data suggest increasing dietary SBH in grow-finish pig diets decreases growth efficiency and the digestibility of GE and N. Further, feeding high inclusion rates of SBH negatively impacts carcass yield, backfat and loin depth.
246 The effect of trypsin inhibitor unit concentrations from raw soybeans in grower pig performance and protein digestibility
Authors: Miller Kayla A , Nisley Mitchell J , Mendoza Omarh F , Spencer Joel D , Krishnan Hari B , Gabler Nicholas K 
Published: May 2024

Abstract
Soybeans contain two major protease inhibitors, Kunitz trypsin and Bowman-Birk protease inhibitors, both of which are anti-nutritional components that can be directly linked to a reduction in amino acid bioavailability and growth performance in swine. Therefore, the objective of this study was to determine the level at which trypsin inhibitor units (TIU, expressed as TIU/mg of complete feed), coming from raw soybeans, in pig diets result in reduction in growth performance and digestibility. This study used 45 grower gilts initial body weight (BW) = 39.7 ± 2.1 kg] that were individually penned and fed one of five dietary TIU treatments (n = 9 pigs/trt) in a complete randomized design. Diets were representative of a commercial corn-soybean meal diet and formulated to have ranging TIU/mg of complete feed of 0.89, 1.77, 3.55, 6.99, and 11.54 TIU/mg utilizing raw ground soybeans and soybean meal at incremental levels. Analyzed diets contained 0.99, 2.23, 3.07, 6.49, and 9.38 active TIU/mg. Diets were balanced to the same grams of SID Lys:ME of 2.85 and NDF concentration of 7.4% utilizing soybean oil and soybean hulls, respectively. Individual feed intake was recorded throughout, and pig BW were collected on d 0, 21, and 27 to calculate average daily gain (ADG), average daily feed intake (ADFI), and feed efficiency gain to feed ratio (G:F). All pigs were placed into metabolism crates on d 21 for 6 d to determine apparent total tract digestibility (ATTD) of nitrogen (N), N excretion and N balance. Growth performance and ATTD data were analyzed with pig as the experimental unit and diet TIU level as a fixed effect using ANOVA, linear, and quadratic contrast models. Overall, ADG decreased as diet TIU increased (1.07, 1.03, 1.02, 0.91, and 0.83 kg/d, respectively, linear P &lt; 0.001). However, no differences in ADFI (P &gt; 0.10) were reported and this resulted in a decrease in G:F (0.48, 0.45, 0.43, 0.41, 0.36, respectively, linear P &lt; 0.001) as dietary TIU concentrations increased from 0.99 to 9.38 TIU/mg. Compared with 0.99 TIU/mg, pigs fed 9.38 TIU/mg had a 9.32% reduction in final BW (68.7 vs. 62.3 kg, linear P &lt; 0.001). Apparent total tract digestibility of N decreased when pigs were fed 9.38 TIU/mg compared with 0.99 and 2.23 TIU/mg (83.26% vs. 90.29% and 89.57%, respectively, P &lt; 0.001). In conclusion, as total trypsin inhibitor units increase in complete feed of grower pigs, digestibility of protein, growth rates and feed efficiency are reduced in a linear manner.
251 Effects of feeding increasing levels of dimethylglycine (DMG) on growth performance, livability, and carcass characteristics of commercial finishing pigs
Authors: Song Ran , Shelton Nick , Schuling Sarah E , Knudtson Sue M , Miller Jodi , Schimek Dan E , Friesen Kim 
Published: May 2024

Abstract
This study aimed to evaluate the impact of increasing dietary levels of 78% sodium dimethylglycine (DMG) on growth performance, livability, and carcass characteristics of commercial finishing pigs. A total of 1,115 mixed-sex finishing pigs [initial body weight (BW) = 70.1 ± 1.8 kg] were housed in pens with 25 to 27 pigs per pen. Pens within block were assigned randomly to one of four dietary treatments containing 0 (Control), 0.25 (DMG_0.25), 0.5 (DMG_0.5) or 1.0 (DMG_1.0) g DMG/kg of feed with 11 replicates per treatment. This experiment extended over an 8-wk period until pigs were harvested at 125.4 ± 0.8 kg of BW. Data were analyzed using the MIXED procedure of SAS, and preplanned orthogonal contrasts were conducted to evaluate pairwise comparisons as well as linear and quadratic effects related to the increased levels of DMG. While no linear effect was observed, there was a tendency for a quadratic response in closeout feed efficiency (G/F; P = 0.08) and the percentage of pigs with morbidity (P = 0.06), with the most favorable responses found in pigs fed DMG_0.25 (Table 1). Pairwise comparisons between Control and DMG_0.25 treatments suggest that pigs receiving DMG_0.25 showed a 6% increase in closeout average daily gain (ADG; 1.01 vs. 0.95 kg/d; P = 0.02) and a 4% increase in closeout G/F (0.337 vs. 0.324; P = 0.05) compared with those fed Control diet. Furthermore, DMG supplemented at 0.25 g/kg tended to be associated with decreased mortality (P = 0.08), reduced morbidity (P = 0.09) and more pigs sold to the primary market (P = 0.10) compared with Control. In contrast, dietary supplementation of greater concentrations of DMG at 0.5 and 1.0 g/kg were not different compared with Control (P &gt; 0.10). Carcass characteristics, including hot carcass weight, carcass yield, fat depth, loin depth, and fat-free carcass lean, were not different among treatments. The findings of this study suggest dietary supplementation of DMG at 0.25 g/kg of feed enhanced growth performance and livability in finishing pigs. However, feeding greater concentrations of DMG, up to 1.0 g/kg of feed, did not produce different outcomes compared with Control. These results indicate the potential benefits of DMG at specific inclusion rate in optimizing the production and health of finishing pigs.
252 Evaluation of phase feeding and complete diet blending at different standardized ileal digestible lysine levels on finishing pig growth performance, carcass characteristics and diet costs
Authors: Navales Ron Aldwin S , Tokach Mike D , Krogstad Dwayne , DeRouchey Joel M , Gebhardt Jordan T , Goodband Robert D , Woodworth Jason C 
Published: May 2024

Abstract
A total of 2,160 mixed-gender pigs [PIC 337 × 1050; initial body weight (BW) = 24.9 ± 0.97 kg) were used in the 120-d study to compare feeding strategies (phase feeding vs complete diet blending) at different SID Lys levels (90 vs 100% of suggested requirement estimates) on finishing pig growth performance, carcass characteristics, and economics. Pens of 27 pigs were assigned to 1 of 4 treatments in a completely randomized block design with barn and initial BW as blocking factors resulting in 20 pens/treatment. The treatments included two feeding programs: a 5-phase feeding strategy at either 90% (Phase-90) or 100% of SID Lys requirement estimates (Phase-100) or blending of complete diets with pre-defined mixing proportions of a low and high SID Lys diet to meet 90 (Blend-90) or 100% (Blend-100) of the SID Lys curve requirement estimates for 23 to 127 kg pigs. Lysine levels were based on PIC 2021 requirement estimates. The 90% SID Lys diets were formulated to contain 4.38, 3.73, 3.06, 2.66 and 2.44 g SID Lys per Mcal NE for phases 1 to 5, respectively. The 100% SID Lys diets were formulated to contain 4.86, 4.15, 3.40, 2.96 and 2.71 g SID Lys per Mcal NE for phases 1 to 5, respectively. Pigs in phase-feeding strategies were fed on a feed budget with 21, 65, 67, 63 and 62 kg per pig for phases 1 to 5, respectively. Final BW and overall average daily gain (ADG; d 0 to 120) were not affected by the feeding strategy nor by diet SID Lys levels. In contrast, overall average daily feed intake (ADFI0 of pigs fed by diet blending was less than those fed by phase feeding (P = 0.002) resulting in increased gain to feed ratio (G:F; P &lt; 0.001) for pigs fed by diet blending (table). The SID Lys levels did not influence overall ADFI or G:F. Hot carcass weight, carcass yield, percentage lean, fat depth and loin depth were not affected by the feeding program. Despite the decreased overall ADFI and increased G:F of pigs fed by diet blending, feeding strategy resulted in no significant differences in economic criteria except for reduced feed cost per kg gain at high price scenario (P = 0.049) for diet blending. With low diet cost, the 90% SID Lys level resulted in decreased feed cost per pig and feed cost per kg gain than 100% SID Lys (P &lt; 0.001), but differences were not detected in income over feed cost (IOFC). In conclusion, diet blending at either 90 or 100% of the SID Lys requirement estimate reduced ADFI and increased G:F without impacting average daily gain (ADG) or carcass characteristics. At ingredient prices used in this study, feeding strategies at either 90 or 100% SID Lys did not significantly affect IOFC, but feeding 90% of SID Lys recommendation reduced feed cost.
248 Effects of feeding finishing pig diets differing in Ca:P ratio, added phytase and 25(OH)D3 on growth performance, serum 25(OH)D3, carcass characteristics, and bone characteristics
Authors: Cordoba Hilario M , Woodworth Jason C , Goodband Robert D , DeRouchey Joel M , Tokach Mike D , Gebhardt Jordan T , Bergstrom Jon R 
Published: May 2024

Abstract
A total of 2,160 pigs [337 × 1050, PIC; initial body weight (BW) = 33.2 ± 1.4 kg] were used in a 114-d trial to determine the effects of feeding diets differing in Ca:P ratio and added phytase or phytase and 25(OH)D3 (Hy-D, DM Nutritional Products, Parsippany, NJ) on growth performance, carcass characteristics, serum 25(OH)D3, and bone characteristics. Pigs were housed in mixed sex pens with 27 pigs per pen and 20 pens per treatment. The four treatments were assigned to pens in a randomized complete block design and consisted of 1) a high phosphorus (HP) diet with standardized total tract digestibility (STTD) P at 115% of NRC (2012) requirement estimate without added phytase and formulated to a 1.25:1 Ca to P ratio; 2) low phosphorus (LP) diet with STTD P at 80% of NRC requirement without added phytase and formulated to a 1.25:1 Ca to P ratio; 3) HP with phytase (HP+phytase) diet with STTD P at 115% of NRC requirement using 0.125% STTD P release from 600 FYT/kg HiPhorius and 1.1:1 Ca to P ratio; and 4) Diet 3 except 25(OH)D3 replaced most of the vitamin D3 in the diet (HP+25(OH)D3). Diets were fed in 4 phases from 32 to 50, 50 to 75, 75 to 100, and 100 to 133 kg. For overall growth performance, source of vitamin D had no impact on performance. Pigs fed HP diets had improved (P &lt; 0.05) average daily gain (ADG), average daily feed intake (ADFI) and gain to feed ratio (G:F) compared with those fed LP diets, and increased ADFI compared with those fed the HP+phytase diets (Table 1). For 25(OH)D3 measured on d 50, pigs fed the HP diets had increased (P &lt; 0.05) serum 25(OH)D3 compared with pigs fed the LP diets and pigs fed the HP+25(OH)D3 diets had increased (P &lt; 0.05) serum 25(OH)D3 compared with pigs fed the HP+phytase diets. Pigs fed the HP diets had greater (P &lt; 0.05) HCW and percentage lean than those fed LP diets. For bone analysis, pigs fed HP diets had greater (P &lt; 0.05) bone ash (g) and breaking strength than pigs fed LP or HP+phytase and 1.1:1 Ca to P ratio. In conclusion, compared with pigs fed LP diets, HP-fed pigs had improved performance and HCW. Dietary 25(OH)D3 replacing D3 did not impact performance but increased circulating 25(OH)D3.
262 Modulation of vitamin D, calcium and phosphorous metabolism and immune response in pre- and post-weaning piglets under different vitamin D supplementation strategies
Authors: Dalto Danyel Bueno , Villeneuve Geneviève , Guay Frédéric , Matte J Jacques , Lapointe Jérôme 
Published: May 2024

Abstract
This study evaluated the impact of different vitamin D supplementation strategies to pre- and post-weaning piglets on vitamin D, calcium and phosphorous metabolism and immune status. Sixty piglets were selected at d 2 of age [body weight (BW) = 1.71 ± 0.03 kg] and orally supplemented with saline (CTR) or a solution of 25(OH)D3 at d 2, d 8, and d 21 (0.1, 0.2 and 0.3 mg, respectively) along with UVB exposure every second day from d1 4 up to d 21 (HD). From weaning (d 21) until d 42, CTR animals were supplemented with 2,000 UI of vitamin D3/kg of feed and HD animals were supplemented with 2,000 UI 25(OH)D3/kg. Piglets were sacrificed at d 21, d 28, and d 35 (n = 10/treatment/d) for the collection of blood, jejunum, liver, and kidney samples. Serum 25(OH)D3 concentrations were determined by HPLC and the mRNA expression of genes implicated in vitamin D, calcium and phosphorous metabolism and immune status were assessed by quantitative real-time PCR. Data were analyzed using a 2x3 factorial design. Serum 25(OH)D3 concentrations were 2.8, 3.6, and 2.2-fold greater in HD piglets at d 21, 28, and 35 (P ≤ 0.01), respectively (Table 1). No treatment or treatment x time interaction was detected on the expression of genes related to vitamin D metabolism in the jejunum mucosa and liver (P ≥ 0.19). In kidney, HD piglets had greater expression of CYP27B1 (P = 0.09) and decreased expression of CYP24A1 (P = 0.06). Renal EGR1 expression was greater in HD piglets (P = 0.01). No treatment effect was detected on the expression of genes directly implicated in phosphate or calcium reabsorption (P ≥ 0.16). No treatment or treatment x time interaction was detected on the mRNA expression of selected genes related to immune response in the jejunum mucosa, liver, and kidney (P ≥ 0.14). Independently of vitamin D treatments, time effects were detected for gene expression. The mRNA expression of CYP27B1 increased and that of CYP24A1 decreased from d 28 to 35 (P ≤ 0.04) in kidney. Genes related to calcium (TRPV5) and phosphate (NPT2c) reabsorption in kidney had reduced mRNA expression on d 35 compared with d 21 (P ≤ 0.01). Overall, the mRNA expression of genes related to immune response in all studied tissues was lower at d 35 compared with d 21 (P ≤ 0.08). Bodyweight at slaughter was not affected by treatments at d 21, 28, and 35 (P ≥ 0.29). In conclusion, pre-weaning vitamin D supplementation enhanced vitamin D status at weaning and the use of dietary 25(OH)D3 supplementation was more effective in increasing the vitamin D status post-weaning. The mRNA expression results suggest that greater vitamin D status at weaning and during the first 2 wk post-weaning do not affect calcium and phosphate reabsorption in kidney and the immune defense of pre- and post-weaning piglets.
256 Anchor Talk: Accounting for effects of microbial phytase on digestibility of calcium and phosphorus
Authors: Stein Hans H 
Published: May 2024

Abstract
Inclusion of increasing levels of microbial phytase in diets for weanling and growing pigs results in a linear increase in the standardized total tract digestibility (STTD) of Ca and P. In many experiments, maximum effects on STTD of Ca and P were obtained with inclusion of around 1,200 phytase units (FTU) per kg of diet, although some of the newer phytases appear to release Ca and P until at least 2,000 FTU is included in the diets. In most individual feed ingredients and most practical diets, the maximum standardized total tract digestibility (STTD) of P that is obtained by using between 500 and 1,000 FTU per kg diet is around 70%, whereas the STTD of Ca for some ingredients can be over 80% if microbial phytase is used. One reason for the decreased STTD of P than of Ca in diets with maximum dosages of phytase is that dietary Ca prevents some P from being absorbed, and the STTD of P in practical diets, therefore, will never be 100% regardless of phytase inclusion. It is, however, important that the increased STTD of dietary Ca that is caused by phytase is taken into account in diet formulation because of the negative effects of Ca on STTD of P. In addition, at low dietary phytase there is release of more Ca than P, whereas at greater inclusion levels of phytase, there may be a similar release of Ca and P. Release of Ca and P by phytase is also impacted by ingredient composition in the diets because phytase does not have the same effect on all ingredients. In general, ingredients with low concentration of phytate have greater STTD of P if no phytase is used than ingredients with greater concentrations of phytate P, but the response to phytase is greater for ingredients with high concentrations of phytate. Sows also have lower digestibility of Ca and P than growing pigs and the response to phytase in diets for sows is less than in diets for growing pigs. Likewise, there is a linear reduction in phytase effects on STTD of both Ca and P as pigs get older. In contrast, in diets for newly weaned pigs there are specific advantages to using high concentrations of phytase (at least 2,000 FTU) because gastric pH is reduced in diets with phytase, which results in less diarrhea. This effect may be related to increased concentrations of plasma inositol in pigs fed diets with phytase, which may positively impact the immune status of the pigs. In conclusion, use of phytase in diets for pigs results in increased STTD of Ca and P and may have other benefits as well.