bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predictors of a true complete response among disappearing liver metastases from colorectal cancer after chemotherapy
Authors: Auer Rebecca C. , White Rebekah R. , Kemeny Nancy E. , Schwartz Lawrence H. , Shia Jinru , Blumgart Leslie H. , DeMatteo Ronald P. , Fong Yuman , Jarnagin William R. , D'Angelica Michael I. 
Published: January 2010

AbstractBACKGROUND:During chemotherapy, some colorectal liver metastases (LMs) disappear on serial imaging. This disappearance may represent a complete response (CR) or a reduction in the sensitivity of imaging during chemotherapy. The objective of the current study was to determine the fate of disappearing LMs (DLMs) and the factors that predict a true CR.METHODS:Between 2000 and 2003, 435 patients who were evaluated by hepatobiliary surgeons received chemotherapy before they were considered for resection. Inclusion criteria were <12 LMs before chemotherapy, at least 1 DLM on a computed tomography (CT) scan, and either surgical resection or 1 year of clinical follow‐up after the disappearance of LMs. A true CR was defined as either a pathologic CR (no tumor detected in the resection specimen) or a durable clinical CR (did not reappear on follow‐up imaging). Clinical and pathologic factors were analyzed to identify those associated with a true CR.RESULTS:During chemotherapy, 39 patients (9%) had a total of 118 DLMs on follow‐up CT scans. Sixty‐eight DLMs were resected, and 50 were followed clinically. Overall, 75 DLMs (64%) were true CRs, including 44 pathologic CRs and 31 durable clinical CRs. On multivariate analysis, the use of hepatic arterial infusion (HAI) chemotherapy (odds ratio [OR], 6.2; P = .02), the inability to observe the DLM on a magnetic resonance image (OR, 4.7; P = .005), and normalization of serum carcinoembryonic antigen levels (OR, 4.6; P = .006) were associated independently with a true CR.CONCLUSIONS:Approximately 66% of DLMs represented a true CR according to assessment by resection or radiologic follow‐up. Predictive factors may help to stratify patients who are likely to harbor residual disease. Cancer 2010. © 2010 American Cancer Society.
Native American breast cancer survivors' physical conditions and quality of life
Authors: Burhansstipanov Linda , Krebs Linda U. , Seals Brenda F. , Bradley Alice A. , Kaur Judith S. , Iron Pamela , Dignan Mark B. , Thiel Carol , Gamito Eduard 
Published: January 2010

AbstractBACKGROUND:Based on Survivors' Guidance, an interactive, Web‐based, culturally relevant Native American cancer survivorship program, Native American Cancer Education for Survivors (NACES), was developed. The focus of the program is to improve quality of life (QOL) for Native American breast cancer survivors.METHODS:NACES is a community‐driven research and education project, based on the Social Cognitive Theoretical Model. Participants complete a QOL survey that includes physical, psychosocial, spiritual, and social components. This publication focuses on the physical component of the survey collected by trained Native American patient advocates, and compares physical conditions among Native American breast cancer survivors who were diagnosed within 1 year, those diagnosed between 1 and 4 years, and those who are long‐term survivors (diagnosed ≥5 years ago).RESULTS:For the first time, survivorship issues are reported specifically for Native American breast cancer patients (n = 266). Selected access issues document situations that contribute to disparities. Comorbidities such as high blood pressure and arthritis are common in the survivors, with more than a third having diabetes, in addition to breast cancer. Numerous side effects from cancer treatments are experienced by these survivors.CONCLUSIONS:These data describe what Native American breast cancer patients are experiencing based on self‐reported information. Clearly there is need for much more work and long‐term tracking of Native American patients to begin to document if or how the severity of physical symptoms lessens over time and if their experiences are significantly different from non‐Native Americans. Cancer 2010. © 2010 American Cancer Society.
Optimizing therapy for patients with chronic myelogenous leukemia in chronic phase
Authors: Kantarjian Hagop M. , Cortes Jorge , La Rosée Paul , Hochhaus Andreas 
Published: January 2010

AbstractIdentification of BCR‐ABL as the defining leukemogenic event in chronic myelogenous leukemia (CML) revolutionized the treatment of the disease. Imatinib, a potent BCR‐ABL inhibitor, is the standard of care for the first‐line treatment of patients with chronic‐phase CML because of its high long‐term response rates and favorable tolerability profile compared with previous standard therapies. However, resistance to imatinib develops in 2% to 4% of patients annually. For patients with acquired cytogenetic resistance to standard‐dose imatinib (400 mg daily), imatinib dose escalation (600‐800 mg daily) is an excellent first option for managing patients and achieving cytogenetic responses. However, for patients with primary resistance to imatinib, hematologic disease recurrence, or emergent BCR‐ABL kinase domain mutations, imatinib dose escalation may not be sufficient to control the disease. For these patients, the potent second‐generation tyrosine kinase inhibitors dasatinib and nilotinib are available. Both agents provide effective therapeutic options for patients with imatinib resistance or intolerance. For the current overview, the authors reviewed the data supporting the use of both dasatinib and nilotinib in imatinib‐resistant or imatinib‐intolerant patients, and they have highlighted the future of CML therapy. Overall, the article is intended to offer physicians a comprehensive review of the current literature and to provide data supporting various treatment options for patients with CML throughout the course of imatinib therapy and beyond. Cancer 2010. © 2010 American Cancer Society.
Pharyngoesophageal reconstruction with the anterolateral thigh flap after total laryngopharyngectomy
Authors: Yu Peirong , Hanasono Matthew M. , Skoracki Roman J. , Baumann Donald P. , Lewin Jan S. , Weber Randal S. , Robb Geoffrey L. 
Published: January 2010

AbstractBACKGROUND:Pharyngoesophageal defects traditionally have been reconstructed using a jejunal or radial forearm flap. In 2002, the authors began using the anterolateral thigh flap for pharyngoesophageal reconstruction, and it has become our preferred method. The purpose of this study was to analyze the clinical and functional outcomes achieved using this technique.METHODS:The medical records of 91 male and 23 female patients who underwent pharyngoesophageal reconstruction using an anterolateral thigh flap were retrospectively reviewed. Outcomes analyzed included length of hospital and intensive care unit stay, fistula and anastomotic stricture formation and other complications, swallowing and tracheoesophageal speech function, and survival. Most patients had primary (27%) or recurrent (42%) squamous cell carcinoma. Before reconstruction, 71% of patients had undergone surgery, radiotherapy, or both. There were 67 circumferential and 47 near‐circumferential defects.RESULTS:Mean intensive care unit stay was 1.9 ± 2.2 days, and mean hospital stay was 9.0 ± 4.7 days. Two patients experienced total flap loss, and 1 patient had partial flap necrosis. Pharyngocutaneous fistulas and strictures occurred in 9% and 6% of patients, respectively. Ninety‐one percent of patients tolerated an oral diet without the need for tube feeding. Tracheoesophageal puncture was performed for speech rehabilitation in 51 patients. Eight‐one percent of patients with a secondary tracheoesophageal puncture achieved fluent speech versus 41% of patients with a primary tracheoesophageal puncture.CONCLUSIONS:This series demonstrates that excellent clinical and functional outcomes, with minimal donor site morbidity and quick recovery, are possible with pharyngoesophageal reconstruction using an anterolateral thigh flap. Cancer 2010. © 2010 American Cancer Society.
Differential distribution of tight junction proteins suggests a role for tanycytes in blood‐hypothalamus barrier regulation in the adult mouse brain
Authors: Mullier Amandine , Bouret Sebastien G. , Prevot Vincent , Dehouck Bénédicte 
Published: January 2010

AbstractThe median eminence is one of the seven so‐called circumventricular organs. It is located in the basal hypothalamus, ventral to the third ventricle and adjacent to the arcuate nucleus. This structure characteristically contains a rich capillary plexus and features a fenestrated endothelium, making it a direct target of blood‐borne molecules. The median eminence also contains highly specialized ependymal cells called tanycytes, which line the floor of the third ventricle. It has been hypothesized that one of the functions of these cells is to create a barrier that prevents substances in the portal capillary spaces from entering the brain. In this paper, we report on our use of immunohistochemistry to study the expression of tight junction proteins in the cells that compose the median eminence in adult mice. Our results indicate that tanycytes of the median eminence express occludin, ZO‐1, and claudin 1 and 5, but not claudin 3. Remarkably, these molecules are organized as a continuous belt around the cell bodies of the tanycytes that line the ventral part of the third ventricle. In contrast, the tanycytes at the periphery of the arcuate nucleus do not express claudin 1 and instead exhibit a disorganized expression pattern of occludin, ZO‐1, and claudin 5. Consistent with these observations, permeability studies using peripheral or central injections of Evans blue dye show that only the tanycytes of the median eminence are joined at their apices by functional tight junctions, whereas tanycytes located at the level of the arcuate nucleus form a permeable layer. In conclusion, this study reveals a unique expression pattern of tight junction proteins in hypothalamic tanycytes, which yields new insights into their barrier properties. J. Comp. Neurol. 518:943–962, 2010. © 2009 Wiley‐Liss, Inc.
Structural, optical, and electrical properties of electron beam evaporated CdSe thin films
Authors: Syed Basheer Ahamed M. G. , Balu A. R. , Nagarethinam V. S. , Thayumanavan A. , Murali K. R. , Sanjeeviraja C. , Jayachandran M. 
Published: January 2010

AbstractCdSe films have been deposited on glass substrates at different substrate temperatures between room temperature and 300 °C. The films exhibited hexagonal structure with preferential orientation in the (002) direction. The crystallinity improved and the grain size increased with temperature. Band gap values are found decreasing from about 1.92 eV to 1.77 eV with increase of the substrate temperature. It is observed that the resistivity decreases continuously with temperature. Laser Raman studies show the presence of 2 LO and 3 LO peaks at 416 cm‐1 and 625 cm‐1respectively. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
1‐Substituted Cyclopentylamines from Nitriles and Tetramethylenebismagnesium Dibromide in the Presence of Ti(OiPr)4
Authors: Tomashenko Olesya A. , Rudenko Andrei E. , Sokolov Viktor V. , Tomashevskiy Alexander A. , de Meijere Armin 
Published: January 2010

AbstractVarious 1‐substituted cyclopentylamines (25 examples, 10–89 % yield) have been prepared according to a one‐pot procedure by the addition of tetramethylenebismagnesium dibromide in the presence of Ti(OiPr)4 to aliphatic, aromatic and heteroaromatic nitriles, respectively.
Silica spheres coated with C18‐modified gold nanoparticles for capillary LC and pressurized CEC separations
Authors: Qu Qishu , Peng Shengwei , Mangelings Debby , Hu Xiaoya , Yan Chao 
Published: January 2010

AbstractNonporous monodispersed silica spheres of 1.3 μm were coated with gold nanoparticles (AuNPs) and subsequently coated with n‐octadecanethiol. By transmission electron microscopy analysis, the average diameter of the AuNPs on the silica spheres was determined to be 12 nm. The chromatographic and electrochromatographic properties of self‐assembled n‐octadecanethiol AuNP‐coated silica microspheres (C18‐AuNPs‐SiO2) were investigated using a group of nonpolar PAHs. The stationary phase appears to display a characteristic reversed‐phase behavior. Higher separation efficiency and shorter separation times were obtained using pressurized CEC (pCEC) compared with capillary LC (CLC). A maximum column efficiency of about 2.5×105 plates per meter and less than 18 min separation time for benzene were obtained in pCEC while only 66 507 plates per meter and an analysis time of nearly 100 min were observed in CLC mode. A chemical stability test of the C18‐AuNPs‐SiO2 stationary phase under extremely high and low pH conditions demonstrated that it is stable at pH 12 and 1 for at least 60 h. The results confirm that C18‐AuNPs‐SiO2 possesses a high rigidity to withstand high packing pressures and can be used as a good stationary phase for CLC and pCEC.
Study on steady‐state kinetics of nucleotide analogues incorporation by non‐gel CE
Authors: Li Wujie , Cao Mou , Pei Lu , Ling Xiaomei , Li Bingchao , Yang Zhenjun 
Published: January 2010

AbstractA method for studying steady‐state kinetics of nucleotide analogues incorporation into DNA strand by non‐gel CE (NGCE) with LIF was developed. Nucleoside analogue is a kind of antiviral agent used to inhibit viral replication in infected cells, especially HIV. Steady‐state parameter Km for nucleotide analogues is determined to imply the relationship between nucleoside analogues and the enzyme in the DNA chain elongation and predict the antiviral efficacy in vivo. Samples were prepared by single nucleotide incorporation assays catalyzed by Taq DNA polymerase at 58°C and HIV reverse transcriptase (RT) at 37°C, and then were separated using NGCE under optimized conditions: 25 mmol/L Tris‐boric‐EDTA buffer (pH 8.0) with 7 mmol/L urea in the presence of 20% w/v PEG 35000 at 30°C and −20 kV. Km(dTTP), Km(d4TTP) and Km(AZTTP) were measured by NGCE for the first time and their values for Taq DNA polymerase were 0.29±0.04, 32.1±3.3 and 74.5±6.6 μmol/L, respectively. For HIV RT, the values were 0.15±0.05, 0.31±0.03 and 0.17±0.03 μmol/L, respectively. The trend of data for HIV RT measured by NGCE was consistent with that measured by PAGE. The reported method by NGCE for the Km determination was powerful, sensitive and fast, and required less amounts of reagents compared with PAGE. It be employed as a reliable alternative method and further applied in other relative studies of nucleoside analogue substrates and DNA polymerases or RTs.
Kinetics of comet formation in single‐cell gel electrophoresis: Loops and fragments
Authors: Afanasieva Katerina , Zazhytska Marianna , Sivolob Andrei 
Published: January 2010

AbstractWe investigated the mechanisms of DNA exit during single‐cell gel electrophoresis (the comet assay) by measuring the kinetics of the comet tail formation. In the neutral comet assay, the rate of DNA exit was found to be dependent on the topological state of DNA, which was influenced by either ethidium bromide or a low radiation dose. The results clearly show that the comet tail is formed by extended DNA loops: the loop extension, being reversible when the DNA torsional constraint remains in the loops, is favored when the constraint is relaxed. The kinetics of the comet formation in the case of a high radiation dose points out that accumulation of the single‐strand breaks causes DNA fragmentation. In contrast to the neutral comet assay, the alkaline comet assay is not related to the chromatin loops. Our results imply that the alkaline treatment induces detachment of the loops from the nuclear matrix, and the comet tail is formed by ssDNA fragments, the ends of which are pulled out from the comet head by electric force. We suggest that the kinetic approach can be considered as an important improvement of the comet assay.
A multichannel gel electrophoresis and continuous fraction collection apparatus for high‐throughput protein separation and characterization
Authors: Choi Megan , Nordmeyer Robert A. , Cornell Earl , Dong Ming , Biggin Mark D. , Jin Jian 
Published: January 2010

AbstractTo facilitate a direct interface between protein separation by PAGE and protein identification by mass spectrometry, we developed a multichannel system that continuously collects fractions as protein bands migrate off the bottom of gel electrophoresis columns. The device was constructed using several short linear gel columns, each of a different percent acrylamide, to achieve a separation power similar to that of a long gradient gel. A “Counter Free‐Flow” elution technique then allows continuous and simultaneous fraction collection from multiple channels at low cost. We demonstrate that rapid, high‐resolution separation of a complex protein mixture can be achieved on this system using SDS‐PAGE. In a 2.5 h electrophoresis run, for example, each sample was separated and eluted into 48–96 fractions over a mass range of ∼10–150 kDa; sample recovery rates were 50% or higher; each channel was loaded with up to 0.3 mg of protein in 0.4 mL; and a purified band was eluted in two to three fractions (200 μL/fraction). Similar results were obtained when running native gel electrophoresis, but protein aggregation limited the loading capacity to about 50 μg per channel and reduced resolution.
Polymer mixtures with enhanced compatibility and extremely low viscosity used as DNA separation media
Authors: Ke Fuyou , Mo Xiulei , Yang Runmiao , Wang Yanmei , Liang Dehai 
Published: January 2010

AbstractPoor compatibility was the major drawback of polymer mixtures when used as DNA separation media. Using poly(ethylene oxide)‐b‐poly(N,N‐dimethylacrylamide) (PEO44‐b‐PDMA88) and PEO (Mw: 1.3 MDa) as an example, we demonstrated the concept that the compatibility was significantly improved when mixing a homopolymer with its copolymer. Laser light scattering indicated that the major interaction between PEO and PEO‐b‐PDMA in dilute solution was the weak hydrodynamic interaction, which showed almost no effect on the viscosity and the static scattering pattern. In semidilute or concentrated solution, viscosity measurement also suggested good compatibility between the two components. When tested as DNA separation medium by CE, the viscosity of the mixture was extremely low, only 5 cP for 5.0 m/v% PEO‐b‐PDMA+0.1 m/v% PEO at 25°C. The performance on DNA separation could be tuned by varying the concentration of each component as well as the component ratio. Good separation on both dsDNA and ssDNA was achieved.
DNA damage and toxicogenomic analyses of hydrogen sulfide in human intestinal epithelial FHs 74 Int cells
Authors: Attene‐Ramos Matias S. , Nava Gerardo M. , Muellner Mark G. , Wagner Elizabeth D. , Plewa Michael J. , Gaskins H. Rex 
Published: January 2010

AbstractHydrogen sulfide (H2S), a metabolic end product of sulfate‐reducing bacteria, represents a genotoxic insult to the colonic epithelium, which may also be linked with chronic disorders such as ulcerative colitis and colorectal cancer. This study defined the early (30 min) and late (4 hr) response of nontransformed human intestinal epithelial cells (FHs 74 Int) to H2S. The genotoxicity of H2S was measured using the single‐cell gel electrophoresis (comet) assay. Changes in gene expression were analyzed after exposure to a genotoxic, but not cytotoxic, concentration of H2S (500 μM H2S) using pathway‐specific quantitative RT‐PCR gene arrays. H2S was genotoxic in a concentration range from 250 to 2,000 μM, which is similar to concentrations found in the large intestine. Significant changes in gene expression were predominantly observed at 4 hr, with the greatest responses by PTGS2 (COX‐2; 7.92‐fold upregulated) and WNT2 (7.08‐fold downregulated). COX‐2 was the only gene upregulated at both 30 min and 4 hr. Overall, the study demonstrates that H2S modulates the expression of genes involved in cell‐cycle progression and triggers both inflammatory and DNA repair responses. This study confirms the genotoxic properties of H2S in nontransformed human intestinal epithelial cells and identifies functional pathways by which this bacterial metabolite may perturb cellular homeostasis and contribute to the onset of chronic intestinal disorders. Environ. Mol. Mutagen. 2010. © 2010 Wiley‐Liss, Inc.
32P‐postlabeling analysis of DNA adducts formed by leukotriene A4 (LTA4)
Authors: Funk Dorothee , Sorg Bernd L. , Lindner Sabine C. , Schmeiser Heinz H. 
Published: January 2010

AbstractLeukotriene A4 (LTA4), a reactive electrophilic intermediate formed during the biosynthesis of inflammation‐related lipid mediators, has been found to bind covalently to DNA. The major DNA adducts formed by LTA4 in vitro and human cells have been identified by mass spectrometry on the nucleoside level. Here we investigated whether the thin‐layer chromatography (TLC) 32P‐postlabeling method is suitable for the detection of LTA4‐DNA adducts. The reaction of individual deoxynucleoside 3′‐monophosphates with LTA4 in aqueous basic solution yielded numerous adduct spots when analyzed by the two enrichment procedures of the 32P‐postlabeling method—nuclease P1 digestion and butanol extraction. Highest LTA4‐adduct levels were found with deoxyguanosine 3′‐phosphate (around one adduct per 104 normal nucleotides). Under similar reaction conditions LTA4 (25–320 μM) was incubated with calf thymus DNA, then DNA adduct patterns and levels were determined with the TLC 32P‐postlabeling method using both enrichment versions. The same DNA adduct pattern consisting of up to seven spots was observed with both enrichment versions. DNA adduct formation by LTA4 was concentration‐dependent with major adducts being derived from deoxyguanosine. When a human monocytic cell line (Mono Mac 6) was stimulated with arachidonic acid and calcium ionophore LTA4‐DNA adducts were detected by 32P‐postlabeling. However, the level of these endogenously formed DNA adducts was close to the detection limit (3 ± 2 adducts per 108 normal nucleotides). In summary, the TLC 32P‐postlabeling method is suitable for studying DNA adduct formation by LTA4 and can be used for further investigations on the link between inflammation and cancer. Environ. Mol. Mutagen. 2010. © 2010 Wiley‐Liss, Inc.
Molecular biology of aromatic plants and spices. A review.
Authors: Trindade Helena 
Published: January 2010

AbstractIn recent years, molecular tools have been used to help to elucidate some aspects of genetic diversity in aromatic species, the genetic relationships between different cultivars and comparisons of molecular marker analysis to the chemical composition of plants. In this review, an explanation of the most important techniques involving molecular markers is given. A literature survey on molecular markers is presented, with some examples from aromatic plants and spices. However, understanding what controls flavour and aroma production in plants is not an easy task to accomplish. Several aspects of plant secondary metabolism, in particular volatiles production in aromatic plants, are still unknown. The route from genomics to proteomics is not well documented, although some research with model plants has already been performed. To address the question of the synthesis of volatiles, two different approaches are possible and summarized in this review: first, the biochemical and genetic approach; and second, approaches involving functional genomics. Finally, a brief survey of bioinformatics resources is presented. Copyright © 2010 John Wiley & Sons, Ltd.