bluuebunny/crossref_metadata_2025_split huggingface dataset:
The clinical relevance of Wilms Tumour 1 (WT1) gene mutations in acute leukaemia
Authors: Owen Carolyn , Fitzgibbon Jude , Paschka Peter 
Published: December 2009

AbstractRecurrent genetic aberrations are important predictors of outcome in acute myeloid leukaemia (AML). Numerous novel molecular abnormalities have been identified and investigated in recent years adding to the risk stratification and prognostication of conventional karyotyping. Mutations in the Wilms Tumour 1 (WT1) gene were first described more than a decade ago but their clinical significance has only recently been evaluated. WT1 mutations occur in approximately 10% of adult AML patients at diagnosis and are most frequent in the cytogenetically normal (CN) AML subgroup. These mutations appear to confer a negative prognostic outcome by increasing the risk of relapse and death. Mutation frequency is higher in pediatric patients and also appears to confer a negative impact on relapse and survival. Herein, we discuss the importance of WT1 mutations in AML. Copyright © 2009 John Wiley & Sons, Ltd.
Parents' feelings towards their adoptive and non‐adoptive children
Authors: Glover Marshaun B. , Mullineaux Paula Y. , Deater‐Deckard Kirby , Petrill Stephen A. 
Published: December 2009

AbstractIn the current study, we examined parent gender differences in feelings (negativity and positivity) and perceptions of child behavioural and emotional problems in adoptive and biological parent–child dyads. In a sample of 85 families, we used a novel within‐family adoption design in which one child was adopted and one child was a biological child of the couple, and tested whether the links between parent feelings and child maladjustment included effects of passive gene–environment correlation. Parents reported more negativity and less positivity as well as higher levels of externalizing behaviour for the adopted child compared to the non‐adopted child, although effect sizes were small and no longer statistically significant after correcting for multiple comparisons. Fathers and mothers did not differ significantly in their reports of positive and negative feelings towards their children or in regard to child externalizing and internalizing behaviours. The correlations between parental negativity and positivity and child externalizing and internalizing were similar for fathers and mothers, and for adopted and non‐adopted children. The findings suggest similar parent–child relationship processes for fathers and mothers, and that genetic transmission of behaviour from parent to child does not account for the association between parental warmth and hostility and child‐adjustment problems. Copyright © 2009 John Wiley & Sons, Ltd.
Nerve invasion distance is dependent on laminin γ2 in tumors of pancreatic cancer
Authors: Mitsunaga Shuichi , Fujii Satoshi , Ishii Genichiro , Kinoshita Taira , Hasebe Takahiro , Aoyagi Kazuhiko , Sasaki Hiroki , Ochiai Atsushi 
Published: December 2009

AbstractThe distance of nerve invasion is an important prognostic factor in pancreatic cancer. The extracellular matrix (ECM) of nerve, mainly composed of laminin, collagen IV and anchoring fibrils, might affect nerve invasion. However, this relationship has not been demonstrated. Our study aimed at discovering the promoting factor of nerve invasion within the tumoral ECM. An animal model was established to evaluate the distance of nerve invasion in murine sciatic nerves by intraneural injection of 6 human pancreatic cancer cell lines. mRNA expression of laminins and anchoring fibrils was compared to the distance of nerve invasion for each cancer cell line. A target molecule provided the strong association between mRNA expression and the distance of nerve invasion. To evaluate the role of a target molecule in nerve invasion, protein expression and function were examined using an animal model and surgical cases. Cancer cells with high laminin γ2 mRNA and protein expression in their basement membranes were associated with long nerve invasion. Knockdown of laminin γ2 in cancer cells significantly shortened nerve invasion in the animal model. In 75 patients with pancreatic cancer, a large distance of nerve invasion was associated with high expression levels of laminin γ2 mRNA and basement membranous deposition of laminin γ2 protein. Our results indicate that laminin γ2 plays an important role in nerve invasion. The measurement of the nerve invasion distance in our mouse nerve invasion model is useful for evaluating the molecular mechanisms of nerve invasion.
Overexpression of DDB2 enhances the sensitivity of human ovarian cancer cells to cisplatin by augmenting cellular apoptosis
Authors: Barakat Bassant M. , Wang Qi‐En , Han Chunhua , Milum Keisha , Yin De‐Tao , Zhao Qun , Wani Gulzar , Arafa El‐Shaimaa A. , El‐Mahdy Mohamed A. , Wani Altaf A. 
Published: December 2009

AbstractCisplatin is one of the most widely used anticancer agents, displaying activity against a wide variety of tumors. However, development of drug resistance presents a challenging barrier to successful cancer treatment by cisplatin. To understand the mechanism of cisplatin resistance, we investigated the role of damaged DNA binding protein complex subunit 2 (DDB2) in cisplatin‐induced cytotoxicity and apoptosis. We show that DDB2 is not required for the repair of cisplatin‐induced DNA damage, but can be induced by cisplatin treatment. DDB2‐deficient noncancer cells exhibit enhanced resistance to cell growth inhibition and apoptosis induced by cisplatin than cells with fully restored DDB2 function. Moreover, DDB2 expression in cisplatin‐resistant ovarian cancer cell line CP70 and MCP2 was lower than their cisplatin‐sensitive parental A2780 cells. Overexpression of DDB2 sensitized CP70 cells to cisplatin‐induced cytotoxicity and apoptosisviaactivation of the caspase pathway and downregulation of antiapoptotic Bcl‐2 protein. Further analysis indicates that the overexpression of DDB2 in CP70 cells downregulates Bcl‐2 expression through decreasingBcl‐2mRNA level. These results suggest that ovarian cancer cells containing high level of DDB2 become susceptible to cisplatin by undergoing enhanced apoptosis.
Interactions of aluminum nanoparticles with human epidermal keratinocytes
Authors: Monteiro‐Riviere Nancy A. , Oldenburg Steven J. , Inman Alfred O. 
Published: December 2009

AbstractAluminum nanoparticles (Al NP) have been used in applications as diverse as drug delivery, material surface coatings and an ingredient for solid rocket fuel in military explosives and artillery. Although Al NP are used in many civilian and military applications, the health and safety implications of these nanosize particles are not known. To understand the interactions and biological activity of Al NP in human cells, cultured human neonatal epidermal keratinocytes (HEK) were exposed for 24 h to 50 and 80 nm Al NP in concentrations from 4.0 to 0.0004 mg ml−1 to assess the cytotoxicity and inflammatory potential. UV–Vis measurements and nanoparticle controls revealed that the Al NP interact with the assay dyes. Viability did not decrease in HEK exposed to both the 50 and the 80 nm Al NP at all treatment concentrations with MTT, CellTiter 96® AQueous One (96 AQ) and alamar Blue® (aB) viability assays. The 96 AQ and aB assays interact with the Al NP less than MTT, and proved to be the best assays to use with these Al NP. TEM depicted Al NP localized within the cytoplasmic vacuoles of the cells. Cytokine data was variable, indicating possible nanoparticle interactions with the cytokine assays. These studies illustrate the difficulties involved in assessing the biological safety of nanomaterials such as Al NP due to media‐ and temperature‐dependent particle agglomeration and nanoparticle interactions with biomarkers of cytotoxicity. Copyright © 2009 John Wiley & Sons, Ltd.
Establishment and characteristics of porcine preadipocyte cell lines derived from mature adipocytes
Authors: Nobusue Hiroyuki , Kano Koichiro 
Published: December 2009

AbstractDevelopment of established preadipocyte cell lines, such as 3T3‐L1 and 3T3‐F442A, greatly facilitated the study of molecular mechanisms of adipocyte differentiation under defined conditions. Most of these cell lines are derived from mouse embryos, and preadipocyte cell lines of other species have not yet been maintained in culture long enough to study differentiation under a variety of conditions. This is the first report on the establishment of porcine preadipocyte cell lines derived from mature adipocytes by a simple method, known as ceiling culture, for culturing mature adipocytes in vitro. This cell line can proliferate extensively until the cells become confluent and fully differentiated into mature adipocytes, depending on adipogenic induction. No changes in their differentiation pattern are observed during their propagation, and they have been successfully carried and differentiated for at least 37 passages. This cell line maintains a normal phenotype without transforming spontaneously, even after long‐term maintenance in culture. This achievement may lead to easy establishment of porcine preadipocyte cell lines and novel model systems for studying the mechanisms of adipocyte differentiation and metabolism as a substitute for human preadipocytes. J. Cell. Biochem. 109: 542–552, 2010. © 2009 Wiley‐Liss, Inc.
Ultrastructure of the extensively developed nuchal organs of Laonice bahusiensis (Annelida: Canalipalpata: Spionidae)
Authors: Jelsing Jacob , Eibye‐Jacobsen Danny 
Published: December 2009

AbstractThe nuchal organs of annelid Laonice bahusiensis (Spionidae) from northern Europe have been studied using scanning and transmission electron microscopy. L. bahusiensis is the first spionid species in which extensively developed, continuous nuchal organs are described. The nuchal organs of this genus are the longest known among polychaete annelids. They consist of paired double bands extending from the prostomium on a mid‐dorsal caruncle for about 24–30 setigers. Their microanatomy corresponds to the general structural plan of nuchal organs: there are ciliated supporting cells and bipolar sensory cells with sensory cilia traversing an olfactory chamber. The organs are overlaid by a secondary paving‐stone‐like cover and innervated by one pair of longitudinally elongated nuchal nerves. These findings clearly favor the hypothesis that the paired, extensively developed ciliated structures found in some Spionidae are homologous with the prostomial nuchal organs characteristic of polychaete annelids. J. Morphol. 2010. © 2009 Wiley‐Liss, Inc.
Transoral laser ventricular fold resection and thyroarytenoid myoneurectomy for adductor spasmodic dysphonia: Long‐term outcome
Authors: Su Chih‐Ying , Lai Chi‐Chih , Wu Pei‐Yin , Huang Hsun‐Hsien 
Published: December 2009

AbstractObjectives:A transoral approach to laser myoneurectomy of the thyroarytenoid muscles was recently reported as an effective technique for treatment of adductor spasmodic dysphonia (ASD). The short‐term results were encouraging. In this study, a long‐term surgical outcome of this technique is investigated.Study Design:A prospective clinical series.Methods:Fifty‐two patients with ASD underwent transoral laryngomicrosurgery with a CO2 laser to resect the ventricular folds followed by myoneurectomy of the thyroarytenoid muscles. The nerve fibers of recurrent laryngeal nerve terminating at the thyroarytenoid muscle, which were frequently found branching deeply among the posterior belly of this muscle, were vaporized. Care was taken not to damage the vocal ligaments, lateral cricoarytenoid muscles, or arytenoid cartilages. Pre‐ and postoperative subjective voice assessments, and acoustic and aerodynamic measurements, were performed and statistically analyzed.Results:Twenty‐nine of the 52 patients who were followed up for more than 12 months (range, 12–63 months; mean, 31 months) were studied. Moderate and marked vocal improvement was achieved in 90% (26/29) of the patients. Three patients had 30% improvement, which was reported as unsuccessful. Eight of the 26 patients who were satisfied with their postoperative voice still had a mild strain during phonation. Of the eight patients, seven achieved normal or nearly normal voice quality after revision laser surgery. No significant vocal deficit or paralysis was observed in any patient.Conclusions:After long‐term follow‐up of 31 months on average, approximately 90% of the ASD patients obtained moderate to marked improvement of vocal performance after transoral laser surgery. The long‐lasting outcome is encouraging. Laryngoscope, 2010
Diagnosis and management of pediatric cervicofacial venous malformations: Retrospective review from a vascular anomalies center
Authors: Glade Robert S. , Richter Gresham T. , James Charles A. , Suen James Y. , Buckmiller Lisa M. 
Published: December 2009

AbstractObjectives/Hypothesis:Venous malformations are rare congenital aberrations of vein development frequently presenting in the head and neck. Without treatment, venous malformations cause progressive disfigurement, dysfunction, and bleeding. In this study, we analyzed a cohort of pediatric patients with cervicofacial venous malformations (CFVM), with the goal of developing a treatment algorithm for these complex lesions.Study Design:Eleven‐year retrospective chart review. The setting was a vascular anomalies center at a pediatric tertiary hospital. Nineteen patients (10 male, 9 female), aged 11 months to 17 years, presented with CFVM.Methods:Patient charts were reviewed for demographics, signs and symptoms, timing of first and subsequent interventions, total number and type of interventions performed, and procedural complications. A family questionnaire supplemented outcome measures by determining the perception of disease control.Results:Presenting symptoms for CFVM include growth (100%), disfigurement (63%), pain (58%), respiratory compromise (42%), and dysphagia (32%). A mean of 6.7 interventions were performed per patient (median, 6; range, 2–12), requiring a mean of 0.8 excisions, 4.6 laser treatments, and 1.3 sclerotherapy injections. Average age at first procedure was 8.5 years. Time between treatments averaged 8.9 months. Four complications occurred in 127 procedures (3.1%). Questionnaire responses indicated subjective improvement following therapy. A management algorithm could be developed from therapeutic outcomes.Conclusions:Treatment of CFVM can be safely and successfully performed with a combination of laser therapy, sclerotherapy, and surgical excision. A treatment algorithm involving multiple procedures during childhood can lead to successful management of CFVM. Laryngoscope, 2010
Role of the otolaryngologist in the treatment of struma ovarii
Authors: Wirtz Eric D. , Bothwell Nici , Klem Christopher 
Published: December 2009

AbstractStruma ovarii, a rare germ cell tumor of the ovary composed of >50% thyroid tissue, is traditionally managed by gynecologic surgeons. Although struma ovarii is typically cured by simple excision, otolaryngology‐head and neck surgeons may play a critical role in caring for these patients. In this article, we present two cases of struma ovarii and discuss the role of the otolaryngologist. Laryngoscope, 2010
Blue‐green light generation using high brilliance edge emitting diode lasers
Authors: Jechow A. , Menzel R. , Paschke K. , Erbert G. 
Published: December 2009

AbstractA review about second harmonic generation using edge emitting diode lasers and nonlinear crystals to obtain laser radiation in the blue‐green spectral range is presented. Therefore, pump laser radiation with high brightness and narrow bandwidth is necessary. Thus, this review gives an overview of the advances made with distributed feedback and Bragg reflector lasers, tapered lasers and amplifiers as well as external cavity diode lasers and master oscillator power amplifier schemes to achieve high brilliance emission. Since periodically poled materials have enabled high second harmonic conversion efficiencies with low and moderate pump powers, the review is focused on frequency doubling using those materials. The most commonly used materials, their properties and limitations are discussed briefly. Single pass and resonant SHG setups with waveguide and bulk nonlinear crystals are discussed and an emphasis on building compact and integrated devices is made.
Modulation of cellulosome composition in Clostridium cellulolyticum: Adaptation to the polysaccharide environment revealed by proteomic and carbohydrate‐active enzyme analyses
Authors: Blouzard Jean‐Charles , Coutinho Pedro M. , Fierobe Henri‐Pierre , Henrissat Bernard , Lignon Sabrina , Tardif Chantal , Pagès Sandrine , de Philip Pascale 
Published: December 2009

AbstractClostridium cellulolyticum is a model mesophilic anaerobic bacterium that efficiently degrades plant cell walls. The recent genome release offers the opportunity to analyse its complete degradation system. A total of 148 putative carbohydrate‐active enzymes were identified, and their modular structures and activities were predicted. Among them, 62 dockerin‐containing proteins bear catalytic modules from numerous carbohydrate‐active enzymes' families and whose diversity reflects the chemical and structural complexity of the plant carbohydrate. The composition of the cellulosomes produced by C. cellulolyticum upon growth on different substrates (cellulose, xylan, and wheat straw) was investigated by LC MS/MS. The majority of the proteins encoded by the cip‐cel operon, essential for cellulose degradation, were detected in all cellulosome preparations. In the presence of wheat straw, the natural and most complex of the substrates studied, additional proteins predicted to be involved in hemicellulose degradation were produced. A 32‐kb gene cluster encodes the majority of these proteins, all harbouring carbohydrate‐binding module 6 or carbohydrate‐binding module 22 xylan‐binding modules along dockerins. This newly identified xyl‐doc gene cluster, specialised in hemicellulose degradation, comes in addition of the cip‐cel operon for plant cell wall degradation. Hydrolysis efficiencies determined on the different substrates corroborates the finding that cellulosome composition is adapted to the growth substrate.
Effects of isoflavones equol and genistein on bone quality in a rat osteopenia model
Authors: Sehmisch S. , Erren M. , Kolios L. , Tezval M. , Seidlova‐Wuttke D. , Wuttke W. , Stuermer K. M. , Stuermer E. K. 
Published: December 2009

AbstractPhytoestrogens might be an alternative medication in prophylaxis and treatment of osteoporosis. In this study, the osteoprotective effects of genistein (GEN) and equol (EQO) were evaluated. After ovariectomy, 44 rats received soy‐free food (Control, C) and developed substantial osteoporosis over the course of two months. After that period, the rats were divided into different groups and fed estradiol (E), GEN or EQO for 35 days. To analyze the osteoprotective effects of the tested substances, bone biomechanical properties and histomorphometric changes of the lumbar vertebrae were evaluated.In analyzing the vertebral body compression strength, we found that the EQO (103.8%) and GEN (96.8%) groups reached similar levels relative to the E group, while the C group reached 77.7% of the biomechanical properties of the E group. EQO was significantly superior to C. The histomorphometric evaluation demonstrated an increased number of nodes in EQO‐ and E‐treated rats compared to GEN‐ and C‐treated rats. E led to an improvement of cortical as well as trabecular bone, an advantage that was only partly seen in the other groups.Treatment with phytoestrogens induced improved bone quality. EQO and GEN might be alternatives for hormone replacement therapy, although further studies are needed to elucidate possible side effects. Copyright © 2009 John Wiley & Sons, Ltd.
Melanin‐concentrating hormone‐1 receptor binding activity of pheophorbides isolated from morus alba leaves
Authors: Oh Byung Koo , Oh Kwang‐Seok , Kwon Kwang‐il , Ryu Shi Yong , Kim Young Sup , Lee Byung Ho 
Published: December 2009

AbstractThe time‐resolved fluorescence technique based on melanin‐concentrating hormone (MCH) receptor subtype‐1 (MCH‐1 receptor) binding assay was adopted to carry out a bioassay‐guided fractionation of the methanol extract of Morus alba leaves. This fractionation and purification led to the isolation of two compounds identified as pheophorbide a methyl ester and 132(S)‐hydroxypheophorbide a methyl ester. These active pheophorbides exhibited potent inhibitory activity in binding of europium‐labeled MCH to the human recombinant MCH‐1 receptor (IC50 value; 4.03 and 0.33 ?M, respectively). Besides binding activity, the pheophorbides inhibited MCH‐mediated extracellular signal‐regulated kinase (ERK) phosphorylation in Chinese hamster ovary cells expressing human MCH‐1 receptor. These results suggest that pheophorbide a methyl ester and 132(S)‐hydroxypheophorbide a methyl ester act as modulators of MCH‐1 receptor and MCH‐mediated ERK signaling. Copyright © 2009 John Wiley & Sons, Ltd.
(‐)‐epigallocatechin‐3‐gallate increases cell proliferation and neuroblasts in the subgranular zone of the dentate gyrus in adult mice
Authors: Yoo Ki‐Yeon , Choi Jung Hoon , Hwang In Koo , Lee Choong Hyun , Lee Soon Ok , Han Sag‐Myung , Shin Hyung‐Cheul , Kang Il‐Jun , Won Moo‐Ho 
Published: December 2009

AbstractNeurogenesis is regulated by several factors such as age, stress and pharmacological agents. We observed the effects of (‐)‐epigallocatechin‐3‐gallate (EGCG), a major catechin of green tea, on neurogenesis in mice. The animals were orally administered EGCG for 4 weeks. Brain sections were stained using a marker for cell proliferation (Ki67 and BrdU) and neuroblasts (doublecortin, DCX). In all groups, Ki67, BrdU and DCX immunoreaction were observed in the subgranular zone of the dentate gyrus. Oral administration of EGCG significantly increased the number of Ki67‐, BrdU‐ and DCX‐immunoreactive cells as well as BrdU/DCX‐colabled cells in the subgranular zone when compared to those in the vehicle‐treated group. These results indicate that oral administration of EGCG can enhance cell proliferation and increase the number of neuroblasts in mice hippocampal dentate gyrus. Copyright © 2009 John Wiley & Sons, Ltd.
Theoretical analysis on the structure of Nb‐doped SrBi4Ti4O15
Authors: Hao Hua , Liu Han‐Xing , Min Xin‐Min , Ouyang Shi‐Xi 
Published: December 2009

AbstractThe structure of niobium‐doped SrBi4Ti4O15 was calculated by using density function and discrete variation method (DFT–DVM). By comparing the total energy of different doping sites, the total energy is found to be lower when Nb ion was substituted into the Ti site in the upper perovskite layer, which is far from the two bismuth (Bi) ions in the perovskite layer. The bonding strength of Nb (3)‐O increases and the electronic conductivity of the SBT decreases after Nb doping. © 2009 Wiley Periodicals, Inc. Int J Quantum Chem, 2011
Theoretical study of the interaction between LiNH2 and HMgH
Authors: Wang Wenjun , Li Wenjing , Li Qingzhong 
Published: December 2009

AbstractThe lithium bond between HMgH and LiNH2 has been predicted and characterized with quantum chemical calculations at the MP2/6‐311++G(d,p) level. Upon formation of the lithium bond, both the MgH and LiN bonds are stretched. The LiN bond undergoes a red shift, whereas the MgH bond exhibits a blue shift. The lithium‐bonded complex is controlled mainly by electrostatic and polarization interactions. The binding energy of HMgH with LiNH2 is computed to be 12.47 kcal/mol. The binding of the two molecules is enhanced by the substitution with the methyl group in the Li acceptor, whereas it is weakened by the replacement with whether the electron‐withdrawing group such as F, Cl, CN, NC, or the electron‐donating group (OH and HN2). A negative cooperativity is present in the ternary system of 2LiNH2 and HMgH. The polarization interaction plays an important role in the negative cooperativity. © 2009 Wiley Periodicals, Inc. Int J Quantum Chem, 2011