bluuebunny/crossref_metadata_2025_split huggingface dataset:
A simple method to quantify the coincidence between portal image graticules and radiation field centers or radiation isocenter
Authors: Du Weiliang , Yang James , Luo Dershan , Martel Mary 
Published: April 2010

Purpose:The aim of this study was to develop a computerized method to quantify the coincidence between portal image graticules and radiation field centers or radiation isocenter. Three types of graticules were included in this study: Megavoltage (MV) mechanical graticule, MV electronic portal imaging device digital graticule, and kilovoltage (kV) on‐board imaging digital graticule.Methods:A metal ball bearing (BB) was imaged with MV and kV x‐ray beams in a procedure similar to a Winston–Lutz test. The radiation fields, graticules, and BB were localized in eight portal images using Hough transform‐based computer algorithms. The center of the BB served as a static reference point in the 3D space so that the distances between the graticule centers and the radiation field centers were calculated. The radiation isocenter was determined from the radiation field centers at different gantry angles.Results:Misalignments of MV and kV portal imaging graticules varied with the gantry or x‐ray source angle as a result of mechanical imperfections of the linear accelerator and its imaging system. While the three graticules in this study were aligned to the radiation field centers and the radiation isocenter within 2.0 mm, misalignments of 1.5–2.0 mm were found at certain gantry angles. These misalignments were highly reproducible with the gantry rotation.Conclusions:A simple method was developed to quantify the alignments of portal image graticules directly against the radiation field centers or the radiation isocenter. The advantage of this method is that it does not require the BB to be placed exactly at the radiation isocenter through a precalibrated surrogating device such as room lasers or light field crosshairs. The present method is useful for radiation therapy modalities that require high‐precision portal imaging such as image‐guided stereotactic radiotherapy.
Simple Sari Cloth Filtration of Water Is Sustainable and Continues To Protect Villagers from Cholera in Matlab, Bangladesh
Authors: Huq Anwar , Yunus Mohammed , Sohel Syed Salahuddin , Bhuiya Abbas , Emch Michael , Luby Stephen P. , Russek-Cohen Estelle , Nair G. Balakrish , Sack R. Bradley , Colwell Rita R. 
Published: April 2010

ABSTRACT
A simple method for filtering water to reduce the incidence of cholera was tested in a field trial in Matlab, Bangladesh, and proved effective. A follow-up study was conducted 5 years later to determine whether the filtration method continued to be employed by villagers and its impact on the incidence of cholera. A total of 7,233 village women collecting water daily for their households in Bangladesh were selected from the same study population of the original field trial for interviewing. Analysis of the data showed that 31% of the women used a filter of which 60% used sari filters for household water. Results showed that sari filtration not only was accepted and sustained by the villagers and benefited them, including their neighbors not filtering water, in reducing the incidence of cholera, the latter being an unexpected benefit.

IMPORTANCE
A simple method for filtering pond and river water to reduce the incidence of cholera, field tested in Matlab, Bangladesh, proved effective in reducing the incidence of cholera by 48%. A follow-up study conducted 5 years later showed that 31% of the village women continued to filter water for their households, with both an expected and an unexpected benefit that filtration had both a direct and indirect effect in reducing cholera (chi-square statistic of 1,591.94;
P
= <0.0001). Results of the study showed that the practice of filtration not only was accepted and sustained by the villagers but also benefited those who filtered their water as well as neighbors not filtering water for household use in reducing the incidence of cholera.
Subtropical pupping ground for a cold-water shark
Authors: Campana Steven E. , Joyce Warren , Fowler Mark 
Published: April 2010

Porbeagle sharks ( Lamna nasus ) are large pelagic sharks apparently restricted to the cold temperate waters of the northern and southern hemispheres. Despite considerable knowledge of their biology, their pupping (birthing) grounds have never been identified. Pop-up archival transmission tags applied to 21 sharks off eastern Canada indicated that males and immature sharks of both sexes remained primarily on the continental shelf for periods of up to 348 days after tagging. However, mature female porbeagles migrated up to 2356 km through the winter, at depths down to 1360 m beneath the Gulf Stream, to a subtropical pupping ground in the Sargasso Sea. In addition to this pupping ground being well south of their documented range, the placement of such a key life history stage in international, largely unregulated waters poses problems for the conservation and management of a species that is largely fished in Canadian waters.
Oblique, High-Angle, Listric-Reverse Faulting and Associated Development of Strain: The Wenchuan Earthquake of May 12, 2008, Sichuan, China
Authors: Zhang Pei-Zhen , Wen Xue-ze , Shen Zheng-Kang , Chen Jiu-hui 
Published: April 2010

The 2008 Wenchuan earthquake occurred on imbricate, oblique, steeply dipping, slowly slipping, listric-reverse faults. Measurements of coseismic slip, the distribution of aftershocks, and fault-plane solution of the mainshock all confirm this style of deformation and indicate cascading earthquake rupture of multiple segments, each with coseismic slip occurring in the shallow crust above a depth range of 10 to 12 km. Interactions among three geological units—eastern Tibet, the Longmen Shan, and the Sichuan basin—caused slow strain accumulation in the Longmen Shan so that measurable preearthquake slip was minor. Coseismic deformation, however, took place mostly within the interseismically locked Longmen Shan fault zone. The earthquake may have initiated from slip on a fault plane dipping 30–40° northwest in a depth range from 15 to 20 km and triggered oblique slip on the high-angle faults at depths shallower than 15 km to form the great Wenchuan earthquake.
The Price of Fame: The Impact of Stimulus Familiarity on Proactive Interference Resolution
Authors: Prabhakaran Ranjani , Thompson-Schill Sharon L. 
Published: April 2010

Abstract
Interference from previously learned information, known as proactive interference (PI), limits our memory retrieval abilities. Previous studies of PI resolution have focused on the role of short-term familiarity, or recency, in causing PI. In the present study, we investigated the impact of long-term stimulus familiarity on PI resolution processes. In two behavioral experiments and one event-related fMRI experiment, long-term familiarity was manipulated through the use of famous and nonfamous stimuli, and short-term familiarity was manipulated through the use of recent and nonrecent probe items in an item recognition task. The right middle frontal gyrus demonstrated greater sensitivity to famous stimuli, suggesting that long-term stimulus familiarity plays a role in influencing PI resolution processes. Further examination of the effect of long-term stimulus familiarity on PI resolution revealed a larger behavioral interference effect for famous stimuli, but only under speeded response conditions. Thus, models of memory retrieval—and of the cognitive control mechanisms that guide retrieval processes—should consider the impact of and interactions among sources of familiarity on multiple time scales.
Brain Structure in Young and Old East Asians and Westerners: Comparisons of Structural Volume and Cortical Thickness
Authors: Chee Michael Wei Liang , Zheng Hui , Goh Joshua Oon Soo , Park Denise , Sutton Bradley P. 
Published: April 2010

Abstract
There is an emergent literature suggesting that East Asians and Westerners differ in cognitive processes because of cultural biases to process information holistically (East Asians) or analytically (Westerners). To evaluate the possibility that such differences are accompanied by differences in brain structure, we conducted a large comparative study on cognitively matched young and old adults from two cultural/ethnic groups—Chinese Singaporeans and non-Asian Americans—that involved a total of 140 persons. Young predominantly White American adults were found to have higher cortical thickness in frontal, parietal, and medial-temporal polymodal association areas in both hemispheres. These findings were replicated using voxel-based morphometry applied to the same data set. Differences in cortical thickness observed between young volunteers were not significant in older subjects as a whole. However, group differences were evident when high-performing old were compared. Although the observed differences in gray matter may be rooted in strategic differences in cognition arising from ethnic/cultural differences, alternative explanations involving genetic heritage and environmental factors are also considered.
Lateral Hip Pain in an Athletic Population: Differential Diagnosis and Treatment Options
Authors: Grumet Robert C. , Frank Rachel M. , Slabaugh Mark A. , Virkus Walter W. , Bush-Joseph Charles A. , Nho Shane J. 
Published: April 2010

Context: Historically, the term greater trochanteric pain syndrome has been used to describe a spectrum of conditions that cause lateral-sided hip pain, including greater trochanteric bursitis, snapping iliotibial band, and/or strains or tendinopathy of the abductor mechanism. Diagnosis of these conditions may be difficult because clinical presentations are variable and sometimes inconclusive. Especially difficult is differentiating intrinsic pain from pain referred to the greater trochanteric region. The purposes of this article are to review the relevant anatomy and pathophysiology of the lateral hip. Evidence Acquisition: Data were collected through a thorough review of the literature conducted through a MEDLINE search of all relevant papers between 1980 and January 2010. Results: Recent advances in imaging and an improved understanding of pathomechanics have helped to guide the evaluation, diagnosis, and appropriate treatment for patients presenting with lateral hip pain. Conclusion: Various diagnostic tools and treatment modalities can be used to effectively manage the athletic patient presenting with lateral hip pain.
Munc13-4 is a limiting factor in the pathway required for platelet granule release and hemostasis
Authors: Ren Qiansheng , Wimmer Christian , Chicka Michael C. , Ye Shaojing , Ren Yi , Hughson Frederick M. , Whiteheart Sidney W. 
Published: April 2010

Abstract
Activation-dependent platelet granule release is mediated by integral membrane proteins called soluble N-ethylmaleimide–sensitive fusion protein attachment protein receptors (SNAREs) and their regulators; however, the mechanisms for this process are ill-defined. To further characterize platelet secretion, we analyzed the function of platelets from Unc13dJinx mice. Platelets from these animals lack the putative vesicle priming factor, Munc13-4, and have a severe secretion defect. Release from dense granules was completely ablated and that from α-granules and lysosomes was severely compromised. Unc13dJinx platelets showed attenuated aggregation and, consequently, Unc13dJinx mice had prolonged tail-bleeding times. The secretion defect was not due to altered expression of SNAREs or SNARE regulators, defective granule biogenesis, or faulty platelet activation. The defective release could be rescued by adding recombinant Munc13-4 to permeabilized Unc13dJinx platelets. In wild-type mouse platelets, Munc13-4 levels were lower than those of SNAREs suggesting that Munc13-4 could be a limiting component of the platelets' secretory machinery. Consistently, Munc13-4 levels directly correlated with the extent of granule release from permeabilized platelets and from intact, heterozygous Unc13dJinx platelets. These data highlight the importance of Munc13-4 in platelets and indicate that it is a limiting factor required for platelet secretion and hemostasis.
Gene up-regulation in response to predator kairomones in the water flea, Daphnia pulex
Authors: Miyakawa Hitoshi , Imai Maki , Sugimoto Naoki , Ishikawa Yuki , Ishikawa Asano , Ishigaki Hidehiko , Okada Yasukazu , Miyazaki Satoshi , Koshikawa Shigeyuki , Cornette Richard , Miura Toru 
Published: April 2010

AbstractBackgroundNumerous cases of predator-induced polyphenisms, in which alternate phenotypes are produced in response to extrinsic stimuli, have been reported in aquatic taxa to date. The genusDaphnia(Branchiopoda, Cladocera) provides a model experimental system for the study of the developmental mechanisms and evolutionary processes associated with predator-induced polyphenisms. InD. pulex, juveniles form neckteeth in response to predatory kairomones released byChaoboruslarvae (Insecta, Diptera).ResultsPrevious studies suggest that the timing of the sensitivity to kairomones inD. pulexcan generally be divided into the embryonic and postembryonic developmental periods. We therefore examined which of the genes in the embryonic and first-instar juvenile stages exhibit different expression levels in the presence or absence of predator kairomones. Employing a candidate gene approach and identifying differentially-expressed genes revealed that the morphogenetic factors,Hox3,extradenticleandescargot, were up-regulated by kairomones in the postembryonic stage and may potentially be responsible for defense morph formation. In addition, the juvenile hormone pathway genes,JHAMTandMet, and the insulin signaling pathway genes,InRandIRS-1, were up-regulated in the first-instar stage. It is well known that these hormonal pathways are involved in physiological regulation following morphogenesis in many insect species. During the embryonic stage when morphotypes were determined, one of the novel genes identified by differential display was up-regulated, suggesting that this gene may be related to morphotype determination. Biological functions of the up-regulated genes are discussed in the context of defense morph formation.ConclusionsIt is suggested that, following the reception of kairomone signals, the identified genes are involved in a series of defensive phenotypic alterations and the production of a defensive phenotype.
The transcription factor Krüppel homolog 1is linked to hormone mediated social organization in bees
Authors: Shpigler Hagai , Patch Harland M , Cohen Mira , Fan Yongliang , Grozinger Christina M , Bloch Guy 
Published: April 2010

Abstract

Background
Regulation of worker behavior by dominant queens or workers is a hallmark of insect societies, but the underlying molecular mechanisms and their evolutionary conservation are not well understood. Honey bee and bumble bee colonies consist of a single reproductive queen and facultatively sterile workers. The queens' influences on the workers are mediated largely via inhibition of juvenile hormone titers, which affect division of labor in honey bees and worker reproduction in bumble bees. Studies in honey bees identified a transcription factor, Krüppel-homolog 1 (Kr-h1), whose expression in worker brains is significantly downregulated in the presence of a queen or queen pheromone and higher in forager bees, making this gene an ideal candidate for examining the evolutionary conservation of socially regulated pathways in Hymenoptera.

Results
In contrast to honey bees, bumble bees foragers do not have higher Kr-h1 levels relative to nurses: in one of three colonies levels were similar in nurses and foragers, and in two colonies levels were higher in nurses. Similarly to honey bees, brain Kr-h1 levels were significantly downregulated in the presence versus absence of a queen. Furthermore, in small queenless groups, Kr-h1 levels were downregulated in subordinate workers with undeveloped ovaries relative to dominant individuals with active ovaries. Brain Kr-h1 levels were upregulated by juvenile hormone treatment relative to a vehicle control. Finally, phylogenetic analysis indicates that KR-H1 orthologs are presence across insect orders. Though this protein is highly conserved between honey bees and bumble bees, there are significant differences between orthologs of insects from different orders.

Conclusions
Our results suggest that Kr-h1 is associated with juvenile hormone mediated regulation of reproduction in bumble bees. The expression of this transcription factor is inhibited by the queen and associated with endocrine mediated regulation of social organization in two species of bees. Thus, KR-H1 may transcriptionally regulate a conserved genetic module that is part of a pathway that has been co-opted to function in social behavior, and adjusts the behavior of workers to their social environmental context.
A survey of well conserved families of C2H2 zinc-finger genes in Daphnia
Authors: Seetharam Arun , Bai Yang , Stuart Gary W 
Published: April 2010

Abstract

Background
A recent comparative genomic analysis tentatively identified roughly 40 orthologous groups of C2H2 Zinc-finger proteins that are well conserved in "bilaterians" (i.e. worms, flies, and humans). Here we extend that analysis to include a second arthropod genome from the crustacean, Daphnia pulex.

Results
Most of the 40 orthologous groups of C2H2 zinc-finger proteins are represented by just one or two proteins within each of the previously surveyed species. Likewise, Daphnia were found to possess a similar number of orthologs for all of these small orthology groups. In contrast, the number of Sp/KLF homologs tends to be greater and to vary between species. Like the corresponding mammalian Sp/KLF proteins, most of the Drosophila and Daphnia homologs can be placed into one of three sub-groups: Class I-III. Daphnia were found to have three Class I proteins that roughly correspond to their Drosophila counterparts, dSP1, btd, CG5669, and three Class II proteins that roughly correspond to Luna, CG12029, CG9895. However, Daphnia have four additional KLF-Class II proteins that are most similar to the vertebrate KLF1/2/4 proteins, a subset not found in Drosophila. Two of these four proteins are encoded by genes linked in tandem. Daphnia also have three KLF-Class III members, one more than Drosophila. One of these is a likely Bteb2 homolog, while the other two correspond to Cabot and KLF13, a vertebrate homolog of Cabot.

Conclusion
Consistent with their likely roles as fundamental determinants of bilaterian form and function, most of the 40 groups of C2H2 zinc-finger proteins are conserved in kind and number in Daphnia. However, the KLF family includes several additional genes that are most similar to genes present in vertebrates but missing in Drosophila.
Inhibition of metastasis, angiogenesis, and tumor growth by Chinese herbal cocktail Tien-Hsien Liquid
Authors: Chia Jean-San , Du Jia-Ling , Hsu Wei-Bin , Sun Andy , Chiang Chun-Pin , Wang Won-Bo 
Published: April 2010

AbstractBackgroundAdvanced cancer is a multifactorial disease that demands treatments targeting multiple cellular pathways. Chinese herbal cocktail which contains various phytochemicals may target multiple dys-regulated pathways in cancer cells and thus may provide an alternative/complementary way to treat cancers. Previously we reported that the Chinese herbal cocktail Tien-Hsien Liguid (THL) can specifically induce apoptosis in various cancer cells and have immuno-modulating activity. In this study, we further evaluated the anti-metastatic, anti-angiogenic and anti-tumor activities of THL with a series ofin vitroandin vivoexperiments.MethodsThe migration and invasion of cancer cells and endothelial cells was determined by Boyden chamber transwell assays. The effect of THL on pulmonary metastasis was done by injecting CT-26 colon cancer cells intravenously to syngenic mice. Thein vitroandin vivomicrovessel formation was determined by the tube formation assay and the Matrigel plug assay, respectively. Thein vivoanti-tumor effect of THL was determined by a human MDA-MB-231 breast cancer xenograft model. The expression of metalloproteinase (MMP)-2, MMP-9, and urokinase plasminogen activator (uPA) was measured by gelatin zymography. The expression of HIF-1α and the phosphorylation of ERK1/2 were determined by Western blot.ResultsTHL inhibited the migration and invasion ability of various cancer cellsin vitro, decreased the secretion of MMP-2, MMP-9, and uPA and the activity of ERK1/2 in cancer cells, and suppressed pulmonary metastasis of CT-26 cancer cells in syngenic mice. Moreover, THL inhibited the migration, invasion, and tube formation of endothelial cellsin vitro, decreased the secretion of MMP-2 and uPA in endothelial cells, and suppressed neovascularization in Matrigel plugs in mice. Besides its inhibitory effect on endothelial cells, THL inhibited hypoxia-induced HIF-1α and vascular endothelial growth factor-A expression in cancer cells. Finally, our results show that THL inhibited the growth of human MDA-MB-231 breast cancer xenografts inNOD-SCIDmice. This suppression of tumor growth was associated with decreased microvessel formation and increased apoptosis caused by THL.ConclusionOur data demonstrate that THL had broad-spectra anti-cancer activities and merits further evaluation for its use in cancer therapy.
Malaria in Brazil: an overview
Authors: Oliveira-Ferreira Joseli , Lacerda Marcus VG , Brasil Patrícia , Ladislau José LB , Tauil Pedro L , Daniel-Ribeiro Cláudio Tadeu 
Published: April 2010

Abstract
Malaria is still a major public health problem in Brazil, with approximately 306 000 registered cases in 2009, but it is estimated that in the early 1940s, around six million cases of malaria occurred each year. As a result of the fight against the disease, the number of malaria cases decreased over the years and the smallest numbers of cases to-date were recorded in the 1960s. From the mid-1960s onwards, Brazil underwent a rapid and disorganized settlement process in the Amazon and this migratory movement led to a progressive increase in the number of reported cases. Although the main mosquito vector (Anopheles darlingi) is present in about 80% of the country, currently the incidence of malaria in Brazil is almost exclusively (99,8% of the cases) restricted to the region of the Amazon Basin, where a number of combined factors favors disease transmission and impair the use of standard control procedures. Plasmodium vivax accounts for 83,7% of registered cases, while Plasmodium falciparum is responsible for 16,3% and Plasmodium malariae is seldom observed. Although vivax malaria is thought to cause little mortality, compared to falciparum malaria, it accounts for much of the morbidity and for huge burdens on the prosperity of endemic communities. However, in the last few years a pattern of unusual clinical complications with fatal cases associated with P. vivax have been reported in Brazil and this is a matter of concern for Brazilian malariologists. In addition, the emergence of P. vivax strains resistant to chloroquine in some reports needs to be further investigated. In contrast, asymptomatic infection by P. falciparum and P. vivax has been detected in epidemiological studies in the states of Rondonia and Amazonas, indicating probably a pattern of clinical immunity in both autochthonous and migrant populations. Seropidemiological studies investigating the type of immune responses elicited in naturally-exposed populations to several malaria vaccine candidates in Brazilian populations have also been providing important information on whether immune responses specific to these antigens are generated in natural infections and their immunogenic potential as vaccine candidates. The present difficulties in reducing economic and social risk factors that determine the incidence of malaria in the Amazon Region render impracticable its elimination in the region. As a result, a malaria-integrated control effort - as a joint action on the part of the government and the population - directed towards the elimination or reduction of the risks of death or illness, is the direction adopted by the Brazilian government in the fight against the disease.