bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mixed phenotype acute leukemia of T/myeloid type with a prominent cellular heterogeneity and unique karyotypic aberration 45,XY, dic(11;17)
Authors: COLOVIC M. , COLOVIC N. , JANKOVIC G. , KRAGULJAC KURTOVIC N. , VIDOVIC A. , DJORDJEVIC V. , BOGDANOVIC A. 
Published: December 2011

SummaryIntroduction.  A 26‐yr‐old male patient with mixed phenotype acute leukemia of T/myeloid type with prominent leukemic cell heterogeneity, and the presence of a so far unreported karyotype aberration in this type of acute leukemia 45,XY, dic(11;17)(11qter→11p11.2::17p11.2→17qter) is presented.Methods.  Flow immunocytometry was performed by direct multicolor immunofluorescent technique on bone marrow aspirates. Cytogenetic analyses were performed using G‐banding method by direct preparation of unstimulated bone marrow cells and following 24 hours of culture in RPMI 1540 culture medium with 25% fetal calf serum at 37°CResults.  The flow immunocytometry of bone marrow nucleated cells revealed the existance of three distinct blast cell populations with overlapping immunophenotypes. Predominant blast cell population had an early myeloid phenotype and aberrant expression of CD7 antigen (HLA‐DR+, CD34+, anti‐MPO+, CD117+, CD33+, CD13+, CD7+low, cyCD3−, TdT−). The other two blast cell populations, smaller in cell diameter and less sizable in cell proportion, both shared the T‐lymphoid features. The patient was treated with ADE protocol (etoposide, cytarabine and doxorubicine). A complete remission was achieved and lasted 5 months.Conclusion.  A case of MPAL with complex biological features, 45,XY, dic(11;17)(11qter→11p11.2::17p11.2→17qter) karyotype and an aggressive, therapy‐resistant clinical course, is presented.
Bivalirudin Inhibits Periprocedural Platelet Function and Tissue Factor Expression of Human Smooth Muscle Cells
Authors: Pepke Wojciech , Eisenreich Andreas , Jaster Markus , Ayral Yunus , Bobbert Peter , Mayer Alexander , Schultheiss Heinz‐Peter , Rauch Ursula 
Published: December 2011

SUMMARY Aim: A major concern of stent implantation after percutaneous coronary intervention (PCI) is acute stent thrombosis. Effective inhibition of periprocedural platelet function in patients with coronary artery disease (CAD) leads to an improved outcome. In this study, we examined the periprocedural platelet reactivity after administrating bivalirudin during PCI compared to unfractionated heparin (UFH) administration. Further, the effect of bivalirudin on induced tissue factor (TF) expression in smooth muscle cells (SMC) was determined. Methods: Patients with CAD (n = 58) and double antithrombotic medication were treated intraprocedural with UFH (n = 30) or bivalirudin (n = 28). Platelet activation markers were flow cytometrically measured before and after stenting. The expression of TF in SMC was determined by real‐time PCR and Western blotting. The thrombogenicity of platelet‐derived microparticles and SMC was assessed via a TF activity assay. Results: Bivalirudin significantly diminished the agonist‐induced platelet reactivity post‐PCI. Compared to UFH treatment, the adenosine diphosphate (ADP) and thrombin receptor‐activating peptide (TRAP)‐induced thrombospondin expression post‐PCI was reduced when bivalirudin was administrated during intervention. In contrast to UFH, bivalirudin reduced the P‐selectin expression of unstimulated and ADP‐induced platelets post‐PCI. Moreover, bivalirudin inhibited the thrombin‐, but not FVIIa‐ or FVIIa/FX‐induced TF expression and pro‐coagulant TF activity of SMC. Moreover, bivalirudin reduced the TF activity of platelet‐derived microparticles postinduction with TRAP or ADP. Conclusions: Bivalirudin is better than UFH in reducing periprocedural platelet activation. Moreover, thrombin‐induced TF expression is inhibited by bivalirudin. Thus, bivalirudin seems to be a better anticoagulant during PCI than UFH.
Effect of Tight Blood Glucose Control Versus Conventional Control in Patients with Type 2 Diabetes Mellitus: A Systematic Review with Meta‐Analysis of Randomized Controlled Trials
Authors: Buehler Anna Maria , Cavalcanti Alexandre Biasi , Berwanger Otavio , Figueiro Mabel , Laranjeira Lígia Nasi , Zazula Ana Denise , Kioshi Bruno , Bugano Diogo G. , Santucci Eliana , Sbruzzi Grazielle , Guimaraes Helio Penna , Carvalho Vitor Oliveira , Bordin Silvana Auxiliadora 
Published: December 2011

Summary Tight control of blood glucose reduces cardiovascular events and total mortality is conflicting. To summarize clinical effects of tight versus conventional glucose control in patients with type 2 diabetes. We systematically searched MEDLINE, EMBASE, Cochrane Library, and ISI Web of Knowledge with no limits of language and time. Further trials were searched from the reference lists of identified studies. We included randomized controlled comparing different levels of blood glucose control intensity in type 2 diabetic patients. Two independent reviewers extracted data of eligible studies using standard case report forms. We investigated total mortality, cardiovascular and microvascular events, and hypoglycemia in patients with type 2 diabetes. We used random‐effects models to obtain relative risks (RR) with 95% confidence intervals (CI). We included 6 trials involving 27,654 patients. There was no significant effect of tight blood glucose control on all‐cause mortality (RR 1.03; 95% CI 0.90–1.17) or cardiovascular mortality (RR 1.04; 95% CI 0.83–1.29). Tight glucose control reduced the risk for nonfatal MI (RR 0.85; 95% CI 0.76–0.95), although had no effect on the incidence of nonfatal stroke (RR 1.02; 95% CI 0.88–1.17). For microvascular events, tight glucose control reduced the risk progression of retinopathy (RR 0.80; 95% CI 0.71–0.91), incidence of peripheral neuropathy (RR 0.94; 95% CI 0.89–0.99), and progression of nephropathy (RR 0.55; 95% CI 0.37–0.80), but had not significant effect on the incidence of nephropathy (RR 0.69; 95% CI 0.42–1.14). The risk of severe hypoglycemia increased with tight glucose control (RR 2.39; 95% CI 1.79–3.18). Tight blood glucose control reduces the risk for some macrovascular and microvascular events, without effect on all‐cause mortality and cardiovascular mortality. Tight glucose control increases the risk of severe hypoglycemia.
N‐Acetylcysteine in Preventing Contrast‐Induced Nephropathy Assessed by Cystatin C
Authors: Alioglu Emin , Saygi Serkan , Turk Ugur , Kirilmaz Bahadir , Tuzun Nurullah , Duman Can , Tengiz Istemihan , Yildiz Serkan , Ercan Ertugrul 
Published: December 2011

SummaryAims: Prophylactic oral N‐acetylcysteine (NAC) has been widely used for prevention of contrast‐induced nephropathy (CIN). However, clinical studies have not been demonstrating this effect consistently because of evidence that NAC can alter serum creatinine levels without affecting glomerular filtration rate (GFR). We investigated NAC for the prevention of CIN by monitoring creatinine and cystatin C.Methods: We enrolled 113 patients (49 patients in NAC group and 64 patients in control group) with normal to subnormal GFR who were scheduled for cardiovascular procedures. Patients in NAC group receive acetylcysteine 600 mg twice a day, on the day before and on the day of cardiovascular procedure. All patients received a periprocedural intravenous infusion ("volume expansion") of 1 ml/kg/h with 0.45% saline for 24 h (12 h before and 12 h after exposure to contrast medium). Serum cystatin C and creatinine levels were measured before and at 12, 24, and 48 h after procedure.Results: The incidence of cystatin C‐based CIN was 28.5% (n = 14) in NAC and 23.4% (n = 15) in control group (p = 0.663) and serum creatinine‐based CIN was 12.2% (n = 6) in NAC and 17.2% (n = 11) in control group (P= 0.468). In this study, oral NAC had no effect on the prevention of CIN in patients undergoing cardiovascular procedures.Conclusion: In this study, oral NAC administration does not reduce neither the incidence of cystatin C‐based CIN nor serum creatinine‐based CIN in patients undergoing cardiovascular procedures.
Effect of Depression on the Risk and Severity of Lower Urinary Tract Symptoms in Community‐Dwelling Elderly Korean Men
Authors: PARK Hyoung Keun , PAICK Sung Hyun , KIM Hyeong Gon , LHO Yong Soo , BYUN Seok‐Soo , LEE Seok Bum , PARK Joon Hyuk , LEE Jung Jae , KIM Ki Woong 
Published: December 2011

Objectives: To evaluate the association of the risk and severity of lower urinary tract symptoms (LUTS) and depression diagnosed by neuropsychiatrists according to the DSM‐IV diagnostic criteria using an objective questionnaire within community‐dwelling elderly Korean men.Methods: A total of 392 men who completed urological and psychiatric evaluations as a participant in the Korean Longitudinal Study on Health and Aging were included in this analysis. From each subject, an interview elicited demographic characteristics and medical history, International Prostate Symptom Score was ascertained, and a psychiatric questionnaire was completed. Subjects were analyzed with regard to depression and LUTS severity.Results: The mean age of the subjects was 75 years, 22% were current smokers and 45% were heavy drinkers. Two hundred and twenty‐nine subjects (59%) had moderate to severe LUTS and 6.4% of the subjects were diagnosed with major depressive disorders. Those with depression showed higher International Prostate Symptom Score and lower quality of life than the euthymic group (P = 0.03 and P = 0.02, respectively). Severe LUTS was more prevalent in the depression group compared with the euthymic group (P = 0.01). Moderate to severe LUTS was associated with higher age, lower prevalence of hypertension, and higher prevalence of depression than mild LUTS. Univariate and multivariate analyses identified age, hypertension, and depression as significant prognostic factors for moderate to severe LUTS. Depression was the most significant prognostic factor. Depression was associated with 5.81‐fold increased odds of having moderate to severe LUTS.Conclusion: In older Korean men, depressive symptoms are associated with moderate to severe LUTS.
Immunological Response to Parenteral Vaccination with Recombinant Hepatitis B Virus Surface Antigen Virus-Like Particles Expressing Helicobacter pylori KatA Epitopes in a Murine H. pylori Challenge Model
Authors: Kotiw Michael , Johnson Megan , Pandey Manisha , Fry Scott , Hazell Stuart L. , Netter Hans J. , Good Michael F. , Olive Colleen 
Published: December 2011

ABSTRACTVirus-like particles (VLPs) based on the small envelope protein of hepatitis B virus (HBsAg-S) are immunogenic at the B- and T-cell level. In this study, we inserted overlapping sequences encoding the carboxy terminus of theHelicobacter pylori katAgene product into HBsAg-S. The HBsAg-S–KatA fusion proteins were able to assemble into secretion-competent VLPs (VLP-KatA). The VLP-KatA proteins were able to induce KatA-specific antibodies in immunized mice. The mean total IgG antibody titers 41 days post-primary immunization with VLP-KatA (2.3 × 103) were significantly greater (P< 0.05) than those observed for vaccination with VLP alone (5.2 × 102). Measurement of IgG isotypes revealed responses to both IgG1 and IgG2a (mean titers, 9.0 × 104and 2.6 × 104, respectively), with the IgG2a response to vaccination with VLP-KatA being significantly higher than that for mice immunized with KatA alone (P< 0.05). Following challenge of mice withH. pylori, a significantly reduced bacterial load in the gastric mucosa was observed (P< 0.05). This is the first report describing the use of VLPs as a delivery vehicle forH. pyloriantigens.
Anti-Toxoplasma Antibody Prevalence, Primary Infection Rate, and Risk Factors in a Study of Toxoplasmosis in 4,466 Pregnant Women in Japan
Authors: Sakikawa Makiko , Noda Shunichi , Hanaoka Masachi , Nakayama Hirotoshi , Hojo Satoshi , Kakinoki Shigeko , Nakata Maki , Yasuda Takashi , Ikenoue Tsuyomu , Kojima Toshiyuki 
Published: December 2011

ABSTRACTToxoplasmosis is a zoonosis caused by infection withToxoplasma gondiiand is prevalent worldwide under various climatic conditions. It is usually asymptomatic, but infection in pregnant women can pose serious health problems for the fetus. However, epidemiological information regarding toxoplasmosis in Japanese pregnant women is limited. This study aimed to determine the prevalence of anti-Toxoplasmaantibodies, the primary infection rate, and the risk factors for toxoplasmosis in Japanese pregnant women. We measured anti-Toxoplasmaantibody titers in 4,466 pregnant women over a period of 7.5 years and simultaneously conducted interviews to identify the risk factors for toxoplasmosis. The overall prevalence of anti-Toxoplasmaantibodies was 10.3%, and it was significantly higher in women aged above 35 years. The rate of primaryToxoplasmainfection during pregnancy was estimated to be 0.25%. A possibility of infection in the later stages of pregnancy was identified for those women who were not infected in the early stages. A history of raw meat intake was identified to be a risk factor related to toxoplasmosis. Therefore, to lower the risk of toxoplasmosis, pregnant women should refrain from eating raw and undercooked meat and maintain personal hygiene.
Human Endogenous Retrovirus K(HML-2) Gag- and Env-Specific T-Cell Responses Are Infrequently Detected in HIV-1-Infected Subjects Using Standard Peptide Matrix-Based Screening
Authors: Jones R. Brad , John Vivek M. , Hunter Diana V. , Martin Eric , Mujib Shariq , Mihajlovic Vesna , Burgers Peter C. , Luider Theo M. , Gyenes Gabor , Sheppard Neil C. , SenGupta Devi , Tandon Ravi , Yue Feng-Yun , Benko Erika , Kovacs Colin , Nixon Douglas F. , Ostrowski Mario A. 
Published: December 2011

ABSTRACTT-cell responses to human endogenous retrovirus (HERV) K(HML-2) Gag and Env were mapped in HIV-1-infected subjects using 15mer peptides. Small peptide pools and high concentrations were used to maximize sensitivity. In the 23 subjects studied, only three bona fide HERV-K(HML-2)-specific responses were detected. At these high peptide concentrations, we detected false-positive responses, three of which were mapped to an HIV-1 Gag peptide contaminant. Thus, HERV-K(HML-2) Gag- and Env-specific T-cell responses are infrequently detected by 15mer peptide mapping.
Complete Genome Sequence of Sphingobium sp. Strain SYK-6, a Degrader of Lignin-Derived Biaryls and Monoaryls
Authors: Masai Eiji , Kamimura Naofumi , Kasai Daisuke , Oguchi Akio , Ankai Akiho , Fukui Shigehiro , Takahashi Mikio , Yashiro Isao , Sasaki Hiroki , Harada Takeshi , Nakamura Sanae , Katano Yoko , Narita-Yamada Sachiko , Nakazawa Hidekazu , Hara Hirofumi , Katayama Yoshihiro , Fukuda Masao , Yamazaki Shuji , Fujita Nobuyuki 
Published: December 2011

ABSTRACT

Sphingobium
sp. strain SYK-6 is able to grow on an extensive variety of lignin-derived biaryls and monoaryls, and the catabolic genes for these compounds are useful for the production of industrially valuable metabolites from lignin. Here we report the complete nucleotide sequence of the SYK-6 genome which consists of the 4,199,332-bp-long chromosome and the 148,801-bp-long plasmid.
Whole-Genome Sequences of Borrelia bissettii, Borrelia valaisiana, and Borrelia spielmanii
Authors: Schutzer Steven E. , Fraser-Liggett Claire M. , Qiu Wei-Gang , Kraiczy Peter , Mongodin Emmanuel F. , Dunn John J. , Luft Benjamin J. , Casjens Sherwood R. 
Published: December 2011

ABSTRACT

It has been known for decades that human Lyme disease is caused by the three spirochete species
Borrelia burgdorferi
,
Borrelia afzelii
, and
Borrelia garinii
. Recently,
Borrelia valaisiana
,
Borrelia spielmanii
, and
Borrelia bissettii
have been associated with Lyme disease. We report the complete genome sequences of
B. valaisiana
VS116,
B. spielmanii
A14S, and
B. bissettii
DN127.
Complete Sequences of Plasmids from the Hemolytic-Uremic Syndrome-Associated Escherichia coli Strain HUSEC41
Authors: Künne Carsten , Billion Andre , Mshana Stephen E. , Schmiedel Judith , Domann Eugen , Hossain Hamid , Hain Torsten , Imirzalioglu Can , Chakraborty Trinad 
Published: December 2011

ABSTRACT

The complete and annotated sequences of four plasmids from a historical enteroaggregative Shiga toxin-producing
Escherichia coli
(HUSEC) serotype O104:H4 strain, HUSEC41/01-09591, isolated in 2001 in Germany are reported.
Genome Sequence of the Haloalkaliphilic Methanotrophic Bacterium Methylomicrobium alcaliphilum 20Z
Authors: Vuilleumier Stéphane , Khmelenina Valentina N. , Bringel Françoise , Reshetnikov Alexandr S. , Lajus Aurélie , Mangenot Sophie , Rouy Zoé , Op den Camp Huub J. M. , Jetten Mike S. M. , Dispirito Alan A. , Dunfield Peter , Klotz Martin G. , Semrau Jeremy D. , Stein Lisa Y. , Barbe Valérie , Médigue Claudine , Trotsenko Yuri A. , Kalyuzhnaya Marina G. 
Published: December 2011

ABSTRACT

Methylomicrobium
strains are widespread in saline environments. Here, we report the complete genome sequence of
Methylomicrobium alcaliphilum
20Z, a haloalkaliphilic methanotrophic bacterium, which will provide the basis for detailed characterization of the core pathways of both single-carbon metabolism and responses to osmotic and high-pH stresses. Final assembly of the genome sequence revealed that this bacterium contains a 128-kb plasmid, making
M. alcaliphilum
20Z the first methanotrophic bacterium of known genome sequence for which a plasmid has been reported.
Draft Genome Sequence of Enterococcus mundtii CRL1656
Authors: Magni Christian , Espeche Carolina , Repizo Guillermo D. , Saavedra Lucila , Suárez Cristian A. , Blancato Víctor S. , Espariz Martín , Esteban Luis , Raya Raúl R. , Font de Valdez Graciela , Vignolo Graciela , Mozzi Fernanda , Taranto María Pía , Hebert Elvira M. , Nader-Macías María Elena , Sesma Fernando 
Published: December 2011

ABSTRACT

We report the draft genome sequence of
Enterococcus mundtii
CRL1656, which was isolated from the stripping milk of a clinically healthy adult Holstein dairy cow from a dairy farm of the northwestern region of Tucumán (Argentina). The 3.10-Mb genome sequence consists of 450 large contigs and contains 2,741 predicted protein-coding genes.
Genome Sequence of the Bacteriocin-Producing Lactobacillus curvatus Strain CRL705
Authors: Hebert Elvira María , Saavedra Lucila , Taranto María Pía , Mozzi Fernanda , Magni Christian , Nader María Elena F. , Font de Valdez Graciela , Sesma Fernando , Vignolo Graciela , Raya Raúl R. 
Published: December 2011

ABSTRACT

Lactobacillus curvatus
is one of the most prevalent lactic acid bacteria found in fermented meat products. Here, we present the draft genome sequence of
Lactobacillus curvatus
CRL705, a bacteriocin producer strain isolated from an Argentinean artisanal fermented sausage, which consists of 1,833,251 bp (GC content, 41.9%) and two circular plasmids of 12,342 bp (pRC12; GC, 43.9%) and 18,664 bp (pRC18; GC, 34.4%).
Complete Genome Sequence of the Rice Pathogen Pantoea ananatis Strain PA13
Authors: Choi Okhee , Lim Jae Yun , Seo Young-Su , Hwang Ingyu , Kim Jinwoo 
Published: December 2011

ABSTRACT

Pantoea ananatis
is the causative agent of sheath and grain rot in rice. Here, we present the complete genome sequence of
P. ananatis
strain PA13, originally isolated from a diseased rice grain.
Shotgun Genome Sequence of a Yersinia enterocolitica Isolate from the Philippines
Authors: Klinzing David C. , Matias Ronald R. , Skowronski Evan , Alvarez May , Liles Veni , Dimamay Mark Pierre , Natividad Filipinas F. 
Published: December 2011

ABSTRACT

The first shotgun genome sequence of a microbial pathogen from the Philippines is reported.
Yersinia enterocolitica
subsp.
palearctica
strain PhRBD_Ye1 is the first
Y. enterocolitica
strain sequenced from an animal source, swine, which is a natural source of yersiniosis. The closest phylogenetic match is a human clinical isolate from Germany.
New Species of Madurella, Causative Agents of Black-Grain Mycetoma
Authors: de Hoog G. Sybren , van Diepeningen Anne D. , Mahgoub El-Sheikh , van de Sande Wendy W. J. 
Published: December 2011

ABSTRACT

A new species of nonsporulating fungus, isolated in a case of black-grain mycetoma in Sudan, is described as
Madurella fahalii
. The species is characterized by phenotypic and molecular criteria. Multigene phylogenies based on the ribosomal DNA (rDNA) internal transcribed spacer (ITS), the partial β-tubulin gene (
BT2
), and the RNA polymerase II subunit 2 gene (
RPB2
) indicate that
M. fahalii
is closely related to
Madurella mycetomatis
and
M. pseudomycetomatis
; the latter name is validated according to the rules of botanical nomenclature.
Madurella ikedae
was found to be synonymous with
M. mycetomatis
. An isolate from Indonesia was found to be different from all known species based on multilocus analysis and is described as
Madurella tropicana. Madurella
is nested within the order Sordariales, with
Chaetomium
as its nearest neighbor.
Madurella fahalii
has a relatively low optimum growth temperature (30°C) and is less susceptible to the azoles than other
Madurella
species, with voriconazole and posaconazole MICs of 1 μg/ml, a ketoconazole MIC of 2 μg/ml, and an itraconazole MIC of >16 μg/ml. Since eumycetoma is still treated only with azoles, correct species identification is important for the optimal choice of antifungal therapy.
Molecular and Microbiological Characterization of Clostridium difficile Isolates from Single, Relapse, and Reinfection Cases
Authors: Oka Kentaro , Osaki Takako , Hanawa Tomoko , Kurata Satoshi , Okazaki Mitsuhiro , Manzoku Taki , Takahashi Motomichi , Tanaka Mamoru , Taguchi Haruhiko , Watanabe Takashi , Inamatsu Takashi , Kamiya Shigeru 
Published: December 2011

ABSTRACT

In this study, we investigated the correlation between the microbiological characteristics of
Clostridium difficile
clinical isolates and the recurrence of
C. difficile
-associated disease (CDAD). Twenty
C. difficile
isolates recovered from 20 single infection cases and 53 isolates from 20 recurrent cases were analyzed by pulsed-field gel electrophoresis (PFGE) and PCR ribotyping, and the cytotoxicity, antimicrobial susceptibility, and sporulation/germination rates of the isolates were examined. Recurrent cases were divided into relapse or reinfection cases by the results of
C. difficile
DNA typing. Among the 20 recurrent cases, 16 cases (80%) were identified to be relapse cases caused by the initial strain and the remaining 4 cases (20%) were identified to be reinfection cases caused by different strains. All 73 isolates were susceptible to both vancomycin and metronidazole, but resistance against clindamycin, ceftriaxone, erythromycin, and ciprofloxacin was found in 87.7%, 93.2%, 87.7%, and 100% of the isolates, respectively. No correlations between DNA typing group, cytotoxicity, and sporulation rate of isolates and infection status, i.e., single, relapse, or reinfection, were observed. However, the isolates recovered from relapse cases showed a significantly higher germination rate when incubated in medium lacking the germination stimulant sodium taurocholate. These results indicate that the germination ability of
C. difficile
may be a potential risk factor for the recurrence of CDAD.