bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of hydrogen peroxide‐induced oxidative stress on leukocytes depends on age and physical training in healthy human subjects carrying the same genotypes of antioxidant enzymes' gene polymorphisms
Authors: Miranda‐Vilela Ana Luisa , Alves Penha Cristina Z. , Akimoto Arthur K. , Pereira Luiz Carlos S. , Nazaré Klautau‐Guimarães Maria de , Grisolia Cesar K. 
Published: September 2010

AbstractObjectivesReactive oxygen species account for the background levels of oxidatively damaged DNA in normal tissues. Physical exercise increases oxygen consumption and can cause oxidative stress. This stress can also involve deficient antioxidant defenses, which can be influenced by certain genetic polymorphisms. Because regular exercise is a known inducer of antioxidant enzymes, the objective of this study was to compare, by comet assay, differences in the DNA damage between apparently healthy individuals and trained aerobic sportsmen carrying the same single nucleotide polymorphisms of manganese superoxide dismutase (Val9Ala), catalase (−21A/T), glutathione peroxidase 1 (Pro198Leu), before and after exposing leukocytes from peripheral blood to hydrogen peroxide (H2O2).MethodsAthletes were compared with nonathletes after a situation that promotes reactive oxygen species increase (a race). Blood samples were submitted to genotyping and comet assay, and the athletes and nonathletes were paired according to their gender, age, and MnSOD, CAT, and GPx‐1 genotypes.ResultsFor nonathletes, there was a positive correlation between H2O2 concentrations and DNA damage levels. For athletes, these correlations showed differences between sexes, indicating that running may impose higher oxidative stress on the DNA of women than of men. Significant differences appeared for nonathletes in the comparisons between younger and older age groups after treatment with H2O2 at 250 μM.ConclusionsThis suggests that, for individuals carrying the same genotypes of antioxidant enzymes' genes, the effect of H2O2‐induced oxidative stress depends mainly on age and physical training. It also suggests that aerobic physical training can reduce oxidative damages to DNA, preventing related diseases in older people. Am. J. Hum. Biol., 2010. © 2010 Wiley‐Liss, Inc.
Epidemiology of nonsyndromic conotruncal heart defects in Texas, 1999–2004
Authors: Long Jin , Ramadhani Tunu , Mitchell Laura E. 
Published: September 2010

AbstractINTRODUCTION: Congenital heart defects (CHDs) are the most common structural birth defects, yet their etiology is poorly understood. As there is heterogeneity within the group of CHDs, epidemiologic studies often focus on subgroups, of conditions, such as conotruncal heart defects (CTDs). However, even within these subgroups there may be etiologic heterogeneity. The aim of the present study was to identify and compare maternal and infant characteristics associated with three CTDs: truncus arteriosus (TA), dextro‐transposition of the great arteries (d‐TGA), and tetralogy of Fallot (TOF). METHODS: Data for cases with nonsyndromic TA (n = 78), d‐TGA (n = 438), and TOF (n = 529) from the Texas Birth Defects Registry, 1999–2004, were used to estimate crude and adjusted prevalence ratios, separately for each condition, using Poisson regression. Polytomous logistic regression was used to determine whether the observed associations were similar across the two largest case groups (d‐TGA and TOF). RESULTS: In Texas, 1999–2004, the prevalence of nonsyndromic TA, d‐TGA, and TOF was 0.35, 1.98, and 2.40 per 10,000 live births, respectively. There was evidence of a significant linear increase in the risk of each condition with advancing maternal age (p < 0.01). Significant associations were observed for TA and maternal residence on the Texas‐Mexico border; d‐TGA and infant sex, maternal race/ethnicity, history of previous live birth, and birth year; and TOF and maternal race/ethnicity and education. Further, the associations with some, but not all, of the study variables were significantly different for d‐TGA and TOF. CONCLUSION: These findings add to our limited understanding of the epidemiology of CTDs. Birth Defects Research (Part A), 2010. © 2010 Wiley‐Liss, Inc.
Meta-analysis of intraoperative povidone–iodine application to prevent surgical-site infection
Authors: Fournel I , Tiv M , Soulias M , Hua C , Astruc K , Aho Glélé L S 
Published: September 2010

AbstractBackgroundThe effectiveness of intraoperative povidone–iodine (PVI) application in the reduction of surgical-site infection (SSI) remains controversial. This meta-analysis was performed to assess the effect of intraoperative PVI application compared with no antiseptic solution (saline or nothing) on the SSI rate.MethodsThe meta-analysis included randomized controlled trials that compared intraoperative PVI lavage with no PVI in patients undergoing surgery with SSI as the primary outcome. A fixed-effects or random-effects model was used as appropriate, and heterogeneity was assessed by the Cochran Q and the I2 value.ResultsTwenty-four randomized controlled trials totalling 5004 patients (2465 patients with PVI and 2539 patients without) were included: 15 in the main analysis and nine in the sensitivity analysis. The rate of SSI was 8·0 per cent in the PVI group and 13·4 per cent in the control group. Intraoperative PVI application significantly decreased the SSI rate (relative risk 0·58, 95 per cent confidence interval 0·40 to 0·83; P = 0·003) and consistent results were observed in subgroup analyses according to the method of PVI administration, its timing and the type of surgery.ConclusionThe meta-analysis results suggested that the use of intraoperative PVI reduced rates of SSI.
Visual aesthetics in store environment and its moderating role on consumer intention
Authors: Vieira Valter Afonso 
Published: September 2010

Abstract
The paper goal is to answer the following questions: What are the factors of centrality of visual aesthetic design (CVSA) in store environment? What factor explains better the construct? Is there a relationship between CVSA and consumers' intentions, such as loyalty, satisfaction, minutes, item, and $ spent inside store? What is the moderating role of store visual aesthetic design on consumer intentions? The research design was a 2 × 2 design, between subjects, which we manipulated store arousal (high vs. low) and CVSA (high vs. low) in two studies. The findings supported the theory that there are three CVSA dimensions; that there is a positive relationship between CVSA and consumer satisfaction, loyalty, items bought, minutes visiting the store, and $ spent; and that high (vs. low) CVSA consumers were more discriminating in their intentions, when the environment had a high arousal. The original value is to support the notion that consumers also evaluate, beyond products, visual aesthetic components in retail and that it plays a moderating role on consumer intention.
Copyright © 2010 John Wiley & Sons, Ltd.
15N and 13C Group‐Selective Techniques Extend the Scope of STD NMR Detection of Weak Host–Guest Interactions and Ligand Screening
Authors: Kövér Katalin E. , Wéber Edit , Martinek Tamás A. , Monostori Éva , Batta Gyula 
Published: September 2010

AbstractSaturation transfer difference (STD) is a valuable tool for studying the binding of small molecules to large biomolecules and for obtaining detailed information on the binding epitopes. Here, we demonstrate that the proposed 15N/13C variants of group‐selective, “GS‐STD” experiments provide a powerful approach to mapping the binding epitope of a ligand even in the absence of efficient spin diffusion within the target protein. Therefore, these experimental variants broaden the scope of STD studies to smaller and/or more‐dynamic targets. The STD spectra obtained in four different experimental setups (selective 1H STD, 15N GS‐STD, 13CAr and 13Caliphatic GS‐STD approaches) revealed that the signal‐intensity pattern of the difference spectra is affected by both the type and the spatial distribution of the excited “transmitter” atoms, as well as by the efficiency of the spin‐diffusion‐mediated magnetization transfer. The performance of the experiments is demonstrated on a system by using the lectin, galectin‐1 and its carbohydrate ligand, lactose.
Termolecular Kinetic Model for CO2‐Alkanolamine Reactions: An Overview
Authors: Vaidya P. D. , Kenig E. Y. 
Published: September 2010

AbstractThe CO2 reaction with alkanolamines has received considerable attention by both academia and industry. Commonly, the formation of the carbamate during the CO2 reaction with primary, secondary, and sterically hindered amines is described by a two‐step zwitterion mechanism. Alternatively, a single‐step termolecular reaction mechanism can also be used to govern carbamate formation. The experimental kinetic data for several amine‐based solvents are consistent with this mechanism, and it can satisfactorily explain fractional‐order and higher‐order kinetics. However, up to now, the termolecular reaction mechanism has not been properly discussed. Here, this mechanism is described in detail, a simple procedure to estimate the kinetic parameters is outlined, and the termolecular reaction kinetics for various systems comprising individual and mixed amines is reviewed.
Experimental Investigation on the Characteristics of Polyethylene Glycol/Cement Composites as Thermal Energy Storage Materials
Authors: Li H. , Fang G.‐Y. 
Published: September 2010

AbstractThe polyethylene glycol/cement composites as thermal energy storage materials were prepared by blending polyethylene glycol and cement. In composite materials, polyethylene glycol (PEG) is used as the phase change material for thermal energy storage and cement acts as the supporting material. A Fourier transformation infrared spectroscope (FT‐IR), x‐ray diffractometer (XRD), and scanning electronic microscope (SEM) were used to determine the chemical structure, the crystalloid phase, and microstructure of the polyethylene glycol/cement composites, respectively. The thermal properties and thermal stability were investigated by a differential scanning calorimeter (DSC) and a thermogravimetry analyzer (TGA). The SEM results showed that the polyethylene glycol was well dispersed in the porous network of the cement.
Vapor Pressure of Water in Mixtures with Hydrophilic Ionic Liquids – A Contribution to the Design of Processes for Drying of Gases by Absorption in Ionic Liquids
Authors: Heym F. , Haber J. , Korth W. , Etzold B. J. M. , Jess A. 
Published: September 2010

AbstractThe low water vapor pressures of mixtures of water with the ionic liquids (ILs), [EMIM][EtSO4] and [BEIM][EtSO4], indicate that a process of gas dehydration by absorption in ILs might be an alternative to the classical absorption process with triethylene glycol (TEG). The activity coefficient for an infinite dilution of water in the IL (xIL → 1), which should be low for efficient dehydration, is only about 0.2 for [EMIM][EtSO4] compared to 0.6 for triethylene glycol. In contrast to TEG, losses by evaporation are excluded with ILs as solvents, because they have a negligible vapor pressure. The number of separation stages needed for the absorption in the IL and for the subsequent regeneration of the water‐loaded IL is small, about six and eight, respectively. IL regeneration can be achieved by distillation of water out of the IL (e.g., at 120 °C) and stripping with ambient air, which is not possible in the case of TEG (chemical attack by O2).
Thermodynamic Studies of Chromium Adsorption on Iron Species Generated by Electrocoagulation
Authors: Parga J. R. , Vazquez V. , Gonzalez G. , Cisneros M. M. 
Published: September 2010

AbstractThe protection of the global environment and in particular, the provision of a sustainable source of clean water is a necessity for human survival. Specifically, large quantities of chromium containing compounds are being discharged into the environment. This study has been carried out to determine the feasibility of chromium adsorption on iron species by an Electrocoagulation (EC) process using the Langmuir Isotherm.The full potential of EC with air injection as an alternative wastewater treatment technique to remove chromium from well water shows more than 99 % removal without the addition of any chemical reagents. In this study, X‐Ray Diffraction, Scanning Electron Microscopy, Mössbauer Spectroscopy and Fourier Transform Infrared Spectroscopy are used to characterize the solid products that reveal the expected crystalline iron oxides, i.e., lepidocrocite, magnetite, gohetite, and iron oxide.
Some common misunderstandings in chemometrics
Authors: Kjeldahl Karin , Bro Rasmus 
Published: September 2010

AbstractThis paper describes a number of issues and tools in practical chemometric data analysis that are often either misunderstood or misused. Deciding what are relevant samples and variables, (mis‐)use of common model diagnostics, and interpretational issues are addressed in relation to component models such as PCA and PLS models. Along with simple misunderstandings, the use of chemometric software packages may contribute to the mistakes if not used critically, and it is thus a main conclusion that good data analysis practice requires the analyst to take responsibility and do what is relevant for the given purpose. Copyright © 2010 John Wiley & Sons, Ltd.
Hydroxyl Radical Reactions with Adenine: Reactant Complexes, Transition States, and Product Complexes
Authors: Cheng Qianyi , Gu Jiande , Compaan Katherine R. , Schaefer Henry F. 
Published: September 2010

AbstractIn order to address problems such as aging, cell death, and cancer, it is important to understand the mechanisms behind reactions causing DNA damage. One specific reaction implicated in DNA oxidative damage is hydroxyl free‐radical attack on adenine (A) and other nucleic acid bases. The adenine reaction has been studied experimentally, but there are few theoretical results. In the present study, adenine dehydrogenation at various sites, and the potential‐energy surfaces for these reactions, are investigated theoretically. Four reactant complexes [A⋅⋅⋅OH]. have been found, with binding energies relative to A+OH. of 32.8, 11.4, 10.7, and 10.1 kcal mol−1. These four reactant complexes lead to six transition states, which in turn lie +4.3, −5.4, (−3.7 and +0.8), and (−2.3 and +0.8) kcal mol−1 below A+OH., respectively. Thus the lowest lying [A⋅⋅⋅OH]. complex faces the highest local barrier to formation of the product (AH).+H2O. Between the transition states and the products lie six product complexes. Adopting the same order as the reactant complexes, the product complexes [(AH)⋅⋅⋅H2O]. lie at −10.9, −22.4, (−24.2 and −18.7), and (−20.5 and −17.5) kcal mol−1, respectively, again relative to separated A+OH.. All six A+OH. → (AH).+H2O pathways are exothermic, by −0.3, −14.7, (−17.4 and −7.8), and (−13.7 and −7.8) kcal mol−1, respectively. The transition state for dehydrogenation at N6 lies at the lowest energy (−5.4 kcal mol−1 relative to A+OH.), and thus reaction is likely to occur at this site. This theoretical prediction dovetails with the observed high reactivity of OH radicals with the NH2 group of aromatic amines. However, the high barrier (37.1 kcal mol−1) for reaction at the C8 site makes C8 dehydrogenation unlikely. This last result is consistent with experimental observation of the imidazole ring opening upon OH radical addition to C8. In addition, TD‐DFT computed electronic transitions of the N6 product around 420 nm confirm that this is the most likely site for hydrogen abstraction by hydroxyl radical.
Construction of N‐Glycan Microarrays by Using Modular Synthesis and On‐Chip Nanoscale Enzymatic Glycosylation
Authors: Serna Sonia , Etxebarria Juan , Ruiz Nerea , Martin‐Lomas Manuel , Reichardt Niels‐Christian 
Published: September 2010

AbstractAn effective chemoenzymatic strategy is reported that has allowed the construction, for the first time, of a focused microarray of synthetic N‐glycans. Based on modular approaches, a variety of N‐glycan core structures have been chemically synthesized and covalently immobilized on a glass surface. The printed structures were then enzymatically diversified by the action of three different glycosyltransferases in nanodroplets placed on top of individual spots of the microarray by a printing robot. Conversion was followed by lectin binding specific for the terminal sugars. This enzymatic extension of surface‐bound ligands in nanodroplets reduces the amount of precious glycosyltransferases needed by seven orders of magnitude relative to reactions carried out in the solution phase. Moreover, only those ligands that have been shown to be substrates to a specific glycosyltransferase can be individually chosen for elongation on the array. The methodology described here, combining focused modular synthesis and nanoscale on‐chip enzymatic elongation, could open the way for the much needed rapid construction of large synthetic glycan arrays.
New Boryl Radicals Derived from N‐Heteroaryl Boranes: Generation and Reactivity
Authors: Lalevée Jacques , Blanchard Nicolas , Tehfe Mohamad‐Ali , Chany Anne‐Caroline , Fouassier Jean‐Pierre 
Published: September 2010

AbstractRecently, boryl radicals have been the subject of revived interest. These structures were generated by hydrogen‐abstraction reactions from the corresponding boranes (i.e., from amine or phosphine boranes). However, the classical issue remains their high BH bond‐dissociation energy (BDE), thereby preventing a very efficient hydrogen‐abstraction process. In the present paper, new N‐heteroaryl boranes that exhibiting low BH BDE are presented; excellent hydrogen‐transfer properties have been found. Both the generation and the reactivity of the associated boryl radicals have been investigated through their direct observation in laser flash photolysis. The boryl radical interactions with double bonds, oxygen, oxidizing agent, and alkyl halides have been studied. Some selected applications of N‐heteroaryl boryl radicals as new polymerization‐initiating structures are proposed to evidence their high intrinsic reactivity.
Chemo‐Genetic Optimization of DNA Recognition by Metallodrugs using a Presenter‐Protein Strategy
Authors: Zimbron Jeremy M. , Sardo Alessia , Heinisch Tillmann , Wohlschlager Therese , Gradinaru Julieta , Massa Claudia , Schirmer Tilman , Creus Marc , Ward Thomas R. 
Published: September 2010

AbstractThe mode of action of precious metal anticancer metallodrugs is generally believed to involve DNA as a target. However, the poor specificity of such drugs often requires high doses and leads to undesirable side‐effects. With the aim of improving the specificity of a ruthenium piano‐stool complex towards DNA, we employed a presenter protein strategy based on the biotin–avidin technology. Guided by the X‐ray structure of the assembly of streptavidin and a biotinylated piano‐stool, we explored the formation of metallodrug‐mediated ternary complexes with the presenter protein and DNA. The assemblies bound more strongly to telomere G‐quadruplexes than to double‐stranded DNA; chemo‐genetic modifications (varying the complex or mutating the protein) modulated binding to these targets. We suggest that rational targeting of small molecules by presenter proteins could be exploited to bind metallodrugs to preferred macromolecular targets.
Magnetothermal Studies of a Series of Coordination Clusters Built from Ferromagnetically Coupled {MnII4MnIII6} Supertetrahedral Units
Authors: Nayak Sanjit , Evangelisti Marco , Powell Annie K. , Reedijk Jan 
Published: September 2010

AbstractThree high‐nuclearity mixed valence manganeseII/III coordination clusters, have been synthesised, that is, [MnIII6MnII4(μ3‐O)4(HL1)6(μ3‐N3)3(μ3‐Br)(Br)](N3)0.7/(Br)0.3⋅3 MeCN⋅2 MeOH (1) (H3L1=3‐methylpentan‐1,3,5‐triol), [MnIII11MnII6(μ4‐O)8(μ3‐Cl)4(μ,μ3‐O2CMe)2(μ,μ‐L2)10Cl2.34(O2CMe)0.66(py)3(MeCN)2]⋅7 MeCN (2) (H2L2=2,2‐dimethyl‐1,3‐propanediol and py is pyridine), and [MnIII12MnII7(μ4‐O)8(μ3‐η1N3)8(HL3)12(MeCN)6]Cl2⋅10 MeOH ⋅MeCN (3) (H3L3=2,6‐bis(hydroxymethyl)‐4‐methylphenol) with high ground‐spin states, S=22, 28±1, and 83/2, respectively; their magnetothermal properties have been studied. The three compounds are based on a common supertetrahedral building block as seen in the Mn10 cluster. This fundamental magnetic unit is made up of a tetrahedron of MnII ions with six MnIII ions placed midway along each edge giving an inscribed octahedron. Thus, the fundamental building unit as represented by compound 1 can be described as a Mn10 supertetrahedron. Compounds 2 and 3 correspond to two such units joined by a common edge or vertex, respectively, resulting in Mn17 and Mn19 coordination clusters. Magnetothermal studies reveal that all three compounds show interesting long‐range magnetic ordering at low temperature, originating from negligible magnetic anisotropy of the compounds; compound 2 shows the largest magnetocaloric effect among the three compounds. This is as expected and can be attributed to the presence of a small magnetic anisotropy, and low‐lying excited states in compound 2.
Effect of dialysate temperature on intradialytic hypotension
Authors: Korkor Adel B. , Bretzmann Christine M. , Eastwood Daniel 
Published: September 2010

AbstractBACKGROUNDThe aim of the study was to determine whether lowering the dialysate temperature in hemodialysis patients would result in a decreased frequency of intradialytic hypotension.METHODSDialysate temperature was lowered by 0.5°C increments in seven hypotension‐prone hemodialysis patients. The primary outcomes, measured over 12 weeks, included systolic blood pressure less than 90 mmHg, an interruption in ultrafiltration due to hypotension, and whether target fluid loss was achieved.RESULTSDialysate temperature was lowered by a total of 0.5°C in four patients and 1.0°C in three patients. Results are presented as odds ratios for every 0.5° decrease in dialysate temperature. The odds of systolic blood pressure dropping to below 90 mmHg and the odds that ultrafiltration would be interrupted due to hypotension were lowered to 0.30 and 0.91 times the odds with a higher dialysate temperature (p < .0001 and p < .0074, respectively). The odds of achieving target fluid loss were 3.60 times greater with a lower dialysate temperature (p < .0001).DISCUSSIONA reduction in dialysate temperature by as little as 0.5–1.0°C had a significant effect on intradialytic hypotension in this patient population. We feel that this represents a practical and effective method for managing intradialytic hypotension in hypotension‐prone dialysis patients.