bluuebunny/crossref_metadata_2025_split huggingface dataset:
THE DIVORCED FINANCIAL SPHERES OF BENINESE SPOUSES
Authors: Lemay‐Boucher Philippe , Dagnelie Olivier 
Published: May 2012

AbstractThis paper exploits original data collected in Benin, using both income and expenditure at the individual level. We provide evidence suggesting that husbands and wives do not pool their respective incomes and thus do not make expenditure decisions on the basis of a common budget. As corroborated by numerous anthropological accounts from West Africa, husband and wife are secretive and individually allocate their personal income to private and public goods. We describe a non‐cooperative context that enables us to predict the determinants of spouses' patterns of consumption. Our empirical results confirm that spouses' financial spheres are, to an extent, disconnected. Copyright © 2012 John Wiley & Sons, Ltd.
Preliminary observations and diagnostic value of lipid peak in ovarian thecomas/fibrothecomas using in vivo proton MR spectroscopy at 3T
Authors: Takeuchi Mayumi , Matsuzaki Kenji , Harada Masafumi 
Published: May 2012

AbstractPurpose:To retrospectively evaluate the significance of lipid peak in in vivo proton magnetic resonance (MR) spectroscopy for the diagnosis of ovarian thecomas/fibrothecomas in patients with solid gynecologic tumors exhibiting totally or partially low signal intensity on T2‐weighted images.Materials and Methods:MR spectroscopy was performed in patients with pathologically diagnosed gynecologic tumors at 3T MRI. Single‐voxel MR spectroscopy data were collected from a single square volume of interest that encompassed the gynecologic masses. The lipid concentration level was classified into three classes (high; low; none).Results:A total of 20 gynecologic tumors in 20 patients were evaluated in this study. High lipid peak was observed in all seven thecomas/fibrothecomas, whereas low lipid peak was observed in only one fibroma in 13 nonthecomatous tumors (six benign ovarian tumors and seven subserosal uterine leiomyomas). The presence of lipid peak for the diagnosis of thecomas/fibrothecomas had a sensitivity of 100%, specificity of 92%, positive predictive value of 88%, and negative predictive value of 100%.Conclusion:The high lipid peak reflecting abundant intracellular lipid contents is considered a specific metabolite concentration for thecomas/fibrothecomas. Demonstration of high lipid peak may contribute to the diagnosis of thecomas/fibrothecomas in distinguishing from other benign ovarian fibrous tumors or subserosal uterine leiomyomas. J. Magn. Reson. Imaging 2012;36:907–911. © 2012 Wiley Periodicals, Inc.
HIV replication is associated with increased severity of liver biopsy changes in HIV‐HBV and HIV‐HCV co‐infection
Authors: Audsley Jennifer , du Cros Philipp , Goodman Zachary , McLean Catriona , Mijch Anne , Lewin Sharon R. , Sasadeusz Joe 
Published: May 2012

AbstractHistological parameters were assessed in liver biopsies (n = 48) performed in patients co‐infected with human immunodeficiency virus (HIV) and hepatitis B virus (HBV) and/or hepatitis C virus (HCV) in order to evaluate factors which were associated with significant liver disease. Necroinflammation and fibrosis was scored by the Ishak classification system, and binary logistic regression analysis was used to assess HIV and antiretroviral‐related determinants of necroinflammation and fibrosis. A total of 46 biopsies were included; 33 were from HIV‐positive patients co‐infected with HCV and 15 biopsies were from HIV‐positive patients co‐infected with HBV. One HIV‐positive patient was co‐infected with HBV and HCV. Median biopsy inflammatory grade for the cohort was 8.5 (IQR 6–10), the median fibrosis Stage 2 (IQR 1.8–4), and the median steatosis score was 1 (IQR 0–2). At the univariate level, HIV‐related variables that were significantly associated with more severe biopsy changes were higher HIV RNA at the time of biopsy (associated with inflammatory Grade 10+; P = 0.018) and any exposure to didanasine (ddI) or stavudine (D4T; associated with fibrosis Stage 3+; P = 0.022). HIV RNA at the time of biopsy remained significant at the multivariate level. Patients with HIV hepatitis co‐infection in this cohort had surprisingly mild changes in liver histology, and there were no statistically significant differences between biopsy results in HBV compared to HCV co‐infection. The association between HIV RNA and necroinflammation supports current recommendations for earlier initiation of HAART in patients with HIV‐hepatitis co‐infection. J. Med. Virol. 84: 993–1001, 2012. © 2012 Wiley Periodicals, Inc.
Phylogenetic analysis of the complete genome of 11 BKV isolates obtained from allogenic stem cell transplant recipients in Ireland
Authors: Drew Richard John , Walsh Anne , Laoi Bairbre Ni , Crowley Brendan 
Published: May 2012

AbstractBK polyomavirus (family Polyomaviridae) may cause hemorrhagic cystitis (BKV‐HC) in hematopoietic stem cell transplant recipients. Eleven complete BKV genomes (GenBank accession numbers: JN192431‐JN192441) were sequenced from urine samples of allogenic hematopoietic stem cell transplant recipients and compared to complete BKV genomes in the published literature. Of the 11 isolates, seven (64%) were subgroup Ib‐1, three (27%) isolates belonged to subgroup Ib‐2 and a single isolate belonged to subtype III. The analysis of single‐nucleotide polymorphisms in this study showed that isolates could be subclassified into subtypes I–IV and subgroups Ib‐1 and Ib‐2 on the basis of VP1 of the first part of the Large T‐antigen (LTag). The non‐coding control region (NCCR) of the 11 isolates was also sequenced. These sequences showed that there was consistent sequence homology within subgroups Ib‐1 and Ib‐2. Two new mutations were described in the isolates, G→C at O84 in isolate SJH‐LG‐310, and a deletion at R2‐7 in isolate SJH‐LG‐309. No known transcription factor is thought to be present at the site of either of these mutations. There were no rearrangements seen in isolates and this may be because the patients were not followed up over time. There were five nucleotide positions at which subgroup Ib‐1 isolated differed from subgroup Ib‐2 isolates in the NCCR sequence, O41, P18, P31, R4, and S18. The mutation O41 is present in the promoter granulocyte/macrophage stimulating factor) gene and the P31 mutation is present in the NF‐1 gene. J. Med. Virol. 84: 1037–1048, 2012. © 2012 Wiley Periodicals, Inc.
Do neutralizing antibody responses generated by human papillomavirus infections favor a better outcome of low‐grade cervical lesions?
Authors: Ochi Hiroyuki , Matsumoto Koji , Kondo Kazunari , Oki Akinori , Furuta Reiko , Hirai Yasuo , Yasugi Toshiharu , Takatsuka Naoyoshi , Maeda Hiroo , Mitsuhashi Akira , Fujii Takuma , Kawana Kei , Iwasaka Tsuyoshi , Yaegashi Nobuo , Watanabe Yoh , Nagai Yutaka , Kitagawa Tomoyuki , Kanda Tadahito , Yoshikawa Hiroyuki ,   for Japan HPV And Cervical Cancer (JHACC) Study Group
Published: May 2012

AbstractTo determine the role of neutralizing antibody generated by human papillomavirus (HPV) infections, baseline levels of serum neutralizing antibodies directed against HPV 16 and cervical HPV DNA were determined in 242 unvaccinated women with low‐grade cervical abnormalities, who were then monitored by cytology and colposcopy every 4 months. In women infected with HPV 16 (n = 42), abnormal cytology persisted longer in those positive for HPV 16‐specific neutralizing antibodies at baseline (median time to cytological regression: 23.8 vs. 7.2 months). Progression to cervical precancer (cervical intraepithelial neoplasia grade 3) within 5 years occurred only among women carrying HPV 16‐specific neutralizing antibodies (P = 0.03, log‐rank test). In women infected with types other than HPV 16 (n = 200), detection of HPV 16‐specific neutralizing antibodies was not correlated with disease outcome. In conclusion, development of specific neutralizing antibodies following natural HPV 16 infection did not favor a better outcome of low‐grade cervical lesions induced by HPV 16 or by other types; rather, detection of neutralizing antibodies generated by current infection may reflect viral persistence and thus help identify those who are at high risk of disease progression. J. Med. Virol. 84: 1128–1134, 2012. © 2012 Wiley Periodicals, Inc.
Determinants of response to triple therapy of telaprevir, peginterferon, and ribavirin in previous non‐responders infected with HCV genotype 1
Authors: Akuta Norio , Suzuki Fumitaka , Seko Yuya , Kawamura Yusuke , Sezaki Hitomi , Suzuki Yoshiyuki , Hosaka Tetsuya , Kobayashi Masahiro , Kobayashi Mariko , Saitoh Satoshi , Arase Yasuji , Ikeda Kenji , Kumada Hiromitsu 
Published: May 2012

AbstractPatients who do not achieve sustained virological response to telaprevir/peginterferon (PEG‐IFN)/ribavirin need to be identified. Predictive factors of virological response to the triple therapy in non‐responders to previous PEG‐IFN/ribavirin therapy are not clear. The aims of this study were to determine the predictive factors of virological response to a 24‐week regimen of triple therapy in 15 non‐responders to previous PEG‐IFN/ribavirin therapy among 61 Japanese adults infected with HCV genotype 1. Overall, sustained virological response and end‐of‐treatment response were achieved by 27% and 60%, respectively. Telaprevir‐resistant variants (by direct sequencing) appeared during or after treatment in 82% of patients who did not show sustained virological response, but disappeared at the end of study, except for one patient with resistant variant at baseline. Substitution at aa 70 (Arg70) and type of previous response to PEG‐IFN/ribavirin (partial response) were identified as significant determinants of sustained virological response. In addition, alpha‐fetoprotein level (<10 µg/L) and type of previous response (partial response) were identified as significant determinants of end‐of‐treatment response. Prediction of response to therapy based on the combination of these factors had high sensitivity, specificity, positive, and negative predictive values. In conclusion, this study identified amino acid substitution of the core region, alpha‐fetoprotein level, and type of previous response as predictors of virological response to telaprevir/PEG‐IFN/ribavirin in patients infected with HCV genotype 1b who had not responded to previous PEG‐IFN/ribavirin therapy. J. Med. Virol. 84: 1097–1105, 2012. © 2012 Wiley Periodicals, Inc.
Factors influencing inadequate or suboptimal response to adefovir with or without genotypic resistance
Authors: Zhou Xia , Kuang XueMei , Fan Ke , Zhang ChangJiang , Deng GuoHong , Wang HuiFen , Wang YuMing 
Published: May 2012

AbstractThe aim of the study was to explore the factors in inadequate‐responders to treatment with adefovir (ADV) with or without genotypic resistance. The reverse‐transcriptase (RT) gene of hepatitis B virus (HBV) was sequenced in 161 patients with inadequate‐response to ADV and analyzed for HBV genotypes using a phylogenetic approach. Seventy‐six patients (47.2%) were found to carry the rtA181V/T/S or rtN236T residue substitution, and most of them had viral rebound. In the patients with viral rebound and ADV genotypic resistance, 19 (25.7%) showed rtA181V/T/S + rtN236T substitutions. In the other patients, it was found that HBV genotypes and cirrhosis influenced the selection of ADV‐resistant positions by univariate analysis and multiple logistic regression analysis. The rtN236T was more frequent in patients with genotype B, and the rtA181V/T/S was more common in patients with genotype C (χ2 = 11.543,P = 0.001). Multiple logistic regression analysis showed that the rtN236T and time resistant strains occurred during ADV‐treatment were statistically significant for influencing rtA181 variation types (P = 0.007 andP = 0.024, respectively), and the occurrence of rtA181T was found to be significantly earlier than rtA181V. In conclusion, genotypic resistance was not detected in the majority of primary nonresponders to ADV when compared to the patients with viral rebound. The different HBV genotypes influence the selection of ADV‐resistant mutation positions. The rtA181T occurs more frequently in patients with the rtN236T and it occurs earlier when compared to the rtA181V. These findings suggest that early judgment of adequate response and making a decision for interference in patients treated with ADV are of importance in clinical practice. J. Med. Virol. 84: 1030–1036, 2012. © 2012 Wiley Periodicals, Inc.
The characterization of monoclonal antibodies to human metapneumovirus and the detection of multiple forms of the virus nucleoprotein and phosphoprotein
Authors: Tedcastle Alison B. , Fenwick Fiona , Ingram Ruth E. , King Barney J. , Robinson Mark J. , Toms Geoffrey L. 
Published: May 2012

AbstractLittle is known of the proteome of human metapneumovirus (HMPV). In this study a panel of monoclonal antibodies to the virus have been characterized and used to identify viral proteins present in infected cell lysates. Of thirteen anti‐HMPV monoclonal antibodies four reacted with recombinant fusion glycoprotein and one with recombinant G glycoprotein by immunofuorescence but not in western blots suggesting that they recognize conformation dependent epitopes. The specificity of the remaining antibodies were determined by MALDI/TOF analysis of the proteins they immunoprecipitated from HMPV infected cell lysates and by western blotting. Five MAbs bound to the nucleoprotein and three to the phosphoprotein. In western blots of lysates of cells infected with low passage HMPV, the anti‐nucleoprotein MAbs stained a single polypeptide corresponding in size to the full length nucleocapsid protein. On repeated passage of the virus in cell culture, however, a second, smaller band appeared which may result from internal initiation of translation within the nucleocapsid gene as described for avian metapneumovirus. Antibodies to the phosphoprotein, besides the full length form, also recognized multiple polypeptides in infected cell lysates, with patterns differing for the two subtypes A and B. The possibility that these too may derive by internal initiation of translation is discussed. J. Med. Virol. 84: 1061–1070, 2012. © 2012 Wiley Periodicals, Inc.
Association between human papillomavirus infection and cytological abnormalities during early follow‐up of invasive cervical cancer
Authors: de Azevedo Ana Emília Borges , Carneiro Fabiana Pirani , Neto Florêncio Figueiredo Cavalcante , Bocca Anamélia Lorenzetti , Teixeira Lorenna Sena , de Queiroz Maurício Filho Marco Antônio Freitas , de Padua Nakashima Juliano , de Abreu Wenzel Castro , Muniz‐Junqueira Maria Imaculada , de Magalhães Albino Verçosa 
Published: May 2012

AbstractConsidering the limitations of cytology for detection of residual cervical cancer after radiotherapy, the aim of this study was to evaluate the frequency and viral load of high‐risk HPV in cervical and vaginal samples during the early follow‐up of patients treated for invasive cervical cancer and correlate the results with cytological examination. Conventional cytology and hybrid capture test were performed on cervical and vaginal samples of 52 women with invasive cervical carcinoma 3 months after therapy. High‐risk HPV was detected in 46.1% of the samples and the median and the range of the ratio relative light unit (RLU)/cutoff (CO) (estimated viral load) in positive samples was 1.71 (1–2120.03). No significant difference was observed in viral frequency and in median of the ratio RLU/CO between samples of patients at different stages (I–III) and between cervical samples, from patients treated by exclusive radiotherapy with or without chemotherapy, and vaginal samples, from patients who underwent to hysterectomy and radiotherapy with or without chemotherapy. Cytological abnormalities were more frequent significantly in samples with HPV than in samples without HPV. The viral load was also higher significantly in samples with cytological abnormalities when compared with the samples without cytological abnormalities. In conclusion, HPV detection methods may be useful during the early follow‐up as a complement to conventional cytology for the diagnosis of residual cervical cancer after radiotherapy. J. Med. Virol. 84: 1115–1119, 2012. © 2012 Wiley Periodicals, Inc.
Prevalence of human papillomavirus types in cervical lesions from women in rural Western India
Authors: Deodhar Kedar , Gheit Tarik , Vaccarella Salvatore , Romao Christine Carreira , Tenet Vanessa , Nene Bhagwan M. , Jayant Kasturi , Kelkar Rohini , Malvi Sylla G. , Sylla Bakary S. , Franceschi Silvia , Jeronimo Jose , Shastri Surendra , Sankaranarayanan Rengaswamy , Tommasino Massimo 
Published: May 2012

AbstractCervical cancer is the most common cancer among women in many areas of India which contributes for a fifth of the global burden of disease. Persistent infection with one of the high‐risk human papillomaviruses (HPV) has been established as the cause for cervical cancer and the documentation of the prevalence of HPV types in cervical cancer in different regions of India is useful for a prevention program combining both screening and vaccination. In this study, the HPV type distribution and the frequency of p16INK4a immunoexpression have been determined in 125 cases of inflammatory lesions or grade 1 cervical intraepithelial neoplasia, 74 cases of grade 2, 72 cases of grade 3, and 113 cervical cancer cases diagnosed among women from rural Solapur and Osmanabad districts, Maharashtra. The overall prevalence of high‐risk HPV was 37.6% in inflammatory lesions or grade 1 cervical intraepithelial neoplasia, 63.5% in grade 2, 97.2% in grade 3 and 92% in cervical cancer cases. HPV 16 and HPV 18 were detected in 80.6% of grade 3 cervical intraepithelial neoplasia and 86.5% of cervical cancer cases. 94.7% of the cervical cancer and 84.4% of the high grade lesions with a strong and full thickness staining for p16INK4a were positive for HPV infection; p16INK4a immunoexpression increased with worsening grade of cervical intraepithelial neoplasia. The HPV genotyping data showing a high HPV 16 and 18 prevalence in cancer specimens indicate that prophylactic HPV 16/18 vaccination would have a significant impact on the prevention of cervical cancer in India. J. Med. Virol. 84: 1054–1060, 2012. © 2012 Wiley Periodicals, Inc.
Association of HPV infection and Chlamydia trachomatis seropositivity in cases of cervical neoplasia in Midwest Brazil
Authors: da Silva Barros Narriman Kennia , Costa Maria Cecília , Alves Rosane Ribeiro Figueiredo , Villa Luísa Lina , Derchain Sophie Françoise Mauricette , Zeferino Luiz Carlos , dos Santos Carneiro Megmar Aparecida , Rabelo‐Santos Silvia Helena 
Published: May 2012

AbstractHigh‐risk human papillomavirus (HPV) is considered the main etiological agent for cervical neoplasia. However, the presence of a single type HPV infection alone is unlikely to be sufficient to cause cervical cancer. There is epidemiologic evidence suggesting that HPV and Chlamydia trachomatis play a central role in the etiology of cervical intraepithelial neoplasia and subsequent cervical cancer. To evaluate the HPV prevalence and the seropositivity for C. trachomatis in women referred to the colposcopy clinic due to an abnormal cervical smear and to examine the effect of this association on the severity of cervical neoplasia. Following enrollment, 131 patients underwent colposcopy and biopsies when necessary. HPV DNA was detected by the polymerase chain reaction (PCR) and genotyping was performed by reverse line‐blot hybridization assay. C. trachomatis seropositivity was tested by ELISA for the detection of IgG antibodies. The prevalence of HPV infection was 86.3%. Seropositivity for C. trachomatis was 26%. Thirty‐one women (27.4%) were positive for C. trachomatis antibodies and HPV‐DNA. The most prevalent HPV type in C. trachomatis‐seropositive women were HPV 16 (51.6%) and this HPV type was present mainly in neoplasia cases. Positivity for HPV, particularly HPV types 16 and 18, and C. trachomatis seropositivity was significantly associated with a diagnosis of high grade neoplasia. Borderline significance was observed after adjustment for HPV. C. trachomatis seropositivity is associated with high grade neoplasia in women infected with HPV, mainly when the types 16 and 18 were involved. J. Med. Virol. 84: 1143–1150, 2012. © 2012 Wiley Periodicals, Inc.
Isolation and characterization of a phenol‐degrading Rhodococcus sp. strain AQ5NOL 2 KCTC 11961BP
Authors: Arif N. M. , Ahmad S. A. , Syed M. A. , Shukor M. Y. 
Published: May 2012

AbstractIn this work, we report on the isolation of a phenol‐degrading Rhodococcus sp. with a high tolerance towards phenol. The isolate was identified as Rhodococcus sp. strain AQ5NOL 2, based on 16S rDNA analysis. The strain degraded phenol using the meta pathway, a trait shared by many phenol‐degraders. In addition to phenol biodegradation, the strain was also capable of degrading diesel. Strain AQ5NOL 2 exhibited a broad optimum temperature for growth on phenol at between 20 °C and 35 °C. The best nitrogen sources were ammonium sulphate, glycine or phenylalanine, followed by proline, nitrate, leucine, and alanine (in decreasing efficiency). Strain AQ5NOL 2 showed a high tolerance and degradation capacity of phenol, for it was able to register growth in the presence of 2000 mg l−1 phenol. The growth of this strain on phenol as sole carbon and energy source were modeled using Haldane kinetics with a maximal specific growth rate (μmax) of 0.1102 hr−1, a half‐saturation constant (Ks) of 99.03 mg l−1 or 1.05 mmol l−1, and a substrate inhibition constant (Ki) of 354 mg l−1 or 3.76 mmol l−1. Aside from phenol, the strain could utilize diesel, 2,4‐dinitrophenol and ρ‐cresol as carbon sources for growth. Strain AQ5NOL 2 exhibited inhibition of phenol degradation by Zn2+, Cu2+, Cr6+, Ag+ and Hg2+ at 1 mg l−1.
Expression of chitinase genes of Metarhizium anisopliae isolates in lepidopteran pests and on synthetic media
Authors: Bhanu Prakash G. V. S. , Padmaja V. , Jami Sravan Kumar , Kirti P. B. 
Published: May 2012

AbstractPathogenecity of the well characterized entomopathogenic fungus Metarhizium anisopliae used for biocontrol of a wide range of insect pests secretes hydrolytic enzymes that degrade the host cuticle. The chitinolytic activity of high and low virulent isolates of M. anisopliae was assayed on minimal medium (MM) + colloidal chitin and MM supplemented with insect cuticles. Ex‐ pression pattern of four chitinase genes (chitinase (chi), chi 1, chi 2, chi 3) was profiled during pathogenic stages of the entomopathogen under in vitro and in vivo conditions. Reverse‐transcription polymerase chain reaction (RT‐PCR) analysis confirmed that chitinase cDNAs were expressed during the germination of fungus under nutrient‐deprived conditions. RT‐PCR analysis performed for the four chitinase genes on the two insect hosts Spodoptera litura and Helicoverpa armigera at six developmental stages of the pathogen displayed up‐regulation in S. litura at mycosed and conidiated condition while with H. armigera there was expression only after 48 h of incubation. Differential expression of chi, chi 1 and chi 2 genes in vitro (nitrogen rich and nitrogen limiting media) and in vivo (live insect hosts S. litura and H. armigera) implicate the role of substrate differences in pathogenesis. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)