bluuebunny/crossref_metadata_2025_split huggingface dataset:
Addressing muscle–tendon imbalances in adult male athletes with personalized exercise prescription based on tendon strain
Authors: Weidlich Kolja , Domroes Theresa , Bohm Sebastian , Arampatzis Adamantios , Mersmann Falk 
Published: June 2024

Abstract
Purpose
Imbalances of muscle strength and tendon stiffness can increase the operating strain of tendons and risk of injury. Here, we used a new approach to identify muscle–tendon imbalances and personalize exercise prescription based on tendon strain during maximum voluntary contractions (εmax) to mitigate musculotendinous imbalances in male adult volleyball athletes.

Methods
Four times over a season, we measured knee extensor strength and patellar tendon mechanical properties using dynamometry and ultrasonography. Tendon micromorphology was evaluated through an ultrasound peak spatial frequency (PSF) analysis. While a control group (n = 12) continued their regular training, an intervention group (n = 10) performed exercises (3 × /week) with personalized loads to elicit tendon strains that promote tendon adaptation (i.e., 4.5–6.5%).

Results
Based on a linear mixed model, εmax increased significantly in the control group over the 9 months of observation (pCon = 0.010), while there was no systematic change in the intervention group (pInt = 0.575). The model residuals of εmax, as a measure of imbalances in muscle–tendon adaptation, demonstrated a significant reduction over time exclusively in the intervention group (pInt = 0.007). While knee extensor muscle strength increased in both groups by ~ 8% (pCon < 0.001, pInt = 0.064), only the intervention group showed a trend toward increased normalized tendon stiffness (pCon = 0.824, pInt = 0.051). PSF values did not change significantly in either group (p > 0.05).

Conclusion
These results suggest that personalized exercise prescription can reduce muscle–tendon imbalances in athletes and could provide new opportunities for tendon injury prevention.
LncRNA HOXC-AS3 accelerates malignant proliferation of cervical cancer cells via stabilizing KDM5B
Authors: Li Jie , Hou Fang , Teng Zhenghua , Xia Weiwei , Peng Jie 
Published: June 2024

Abstract
Background
Cervical cancer (CC) is a common malignancy amongst women globally. Ubiquitination plays a dual role in the occurrence and development of cancers. This study analyzed the mechanism of long noncoding RNA HOXC cluster antisense RNA 3 (lncRNA HOXC-AS3) in malignant proliferation of CC cells via mediating ubiquitination of lysine demethylase 5B (KDM5B/JARID1B).

Methods
The expression patterns of lncRNA HOXC-AS3 and KDM5B were measured by real-time quantitative polymerase chain reaction or Western blot analysis. After transfection with lncRNA HOXC-AS3 siRNA and pcDNA3.1-KDM5B, proliferation of CC cells was assessed by the cell counting kit-8, colony formation, and 5-Ethynyl-2’-deoxyuridine staining assays. The xenograft tumor model was established to confirm the impact of lncRNA HOXC-AS3 on CC cell proliferation in vivo by measuring tumor size and weight and the immunohistochemistry assay. The subcellular location of lncRNA HOXC-AS3 and the binding of lncRNA HOXC-AS3 to KDM5B were analyzed. After treatment of lncRNA HOXC-AS3 siRNA or MG132, the protein and ubiquitination levels of KDM5B were determined. Thereafter, the interaction and the subcellular co-location of tripartite motif-containing 37 (TRIM37) and KDM5B were analyzed by the co-immunoprecipitation and immunofluorescence assays.

Results
LncRNA HOXC-AS3 and KDM5B were upregulated in CC tissues and cells. Depletion of lncRNA HOXC-AS3 repressed CC cell proliferation and in vivo tumor growth. Mechanically, lncRNA HOXC-AS3 located in the nucleus directly bound to KDM5B, inhibited TRIM37-mediated ubiquitination of KDM5B, and upregulated the protein levels of KDM5B. KDM5B overexpression attenuated the inhibitory role of silencing lncRNA HOXC-AS3 in CC cell proliferation in vivo and in vitro.

Conclusion
Nucleus-located lncRNA HOXC-AS3 facilitated malignant proliferation of CC cells via stabilization of KDM5B protein levels.
The role of specialist palliative care in individuals “living beyond cancer”: a narrative review of the literature
Authors: Taylor Amy , Davies Andrew 
Published: June 2024

Abstract
Purpose
Many patients living beyond cancer experience significant unmet needs, although few of these patients are currently reviewed by specialist palliative care teams (SPCTs). The aim of this narrative review was to explore the current and potential role of SPCTs in this cohort of patients.

Methods
A search strategy was developed for Medline, and adapted for Embase, CINAHL, and PsycInfo. Additionally, websites of leading oncology, cancer survivorship, and specialist palliative care organisations were examined. The focus of the search was on individuals living beyond cancer rather than other groups of cancer survivors.

Results
111 articles were retrieved from the search for full text review, and 101 other sources of information were identified after hand searching the reference lists of the full text articles, and the aforesaid websites. The themes of the review encompass the definition of palliative care/specialist palliative care, current models of specialist palliative care, core activities of SPCTs, relevant expertise of SPCTs, and potential barriers to change in relation to extending their support and expertise to individuals living beyond cancer. The review identified a paucity of evidence to support the role of SPCTs in the management of patients living beyond cancer.

Conclusions
Individuals living beyond cancer have many unmet needs, and specific services are required to manage these problems. Currently, there is limited evidence to support the role of specialist palliative care teams in the management of this cohort of people, and several potential barriers to greater involvement, including limited resources, and lack of relevant expertise.
Yoga and meditation for menopausal symptoms in breast cancer survivors: a qualitative study exploring participants’ experiences
Authors: Bilc Mirela , Pollmann Nina , Buchholz Analena , Lauche Romy , Cramer Holger 
Published: June 2024

Abstract
Purpose
Breast cancer survivors commonly experience menopausal symptoms, specifically when undergoing antihormonal therapy. Unfortunately, they often have a restricted range of treatment options available to alleviate menopausal symptoms. The objective of this qualitative study was to explore breast cancer survivors’ experiences and effects of a yoga and meditation intervention supplementing previously reported RCT outcomes.

Methods
The qualitative data included in this study were part of a larger randomized controlled trial which evaluated the efficacy and safety of a 12-week yoga and meditation intervention on menopausal symptoms in breast cancer survivors. All participants who underwent the yoga intervention (n = 19) were invited to take part in semi-structured interviews after all quantitative data collection had been completed. Interviews (n = 9) were recorded, transcribed, and then coded into superordinate themes using thematic analysis.

Results
Nine female participants were interviewed, and the following themes emerged: (1) representations and expectations from the yoga intervention; (2) course structure and implementation; (3) perceptions and effects of the intervention (at emotional, physical, behavioral, and spiritual level); (4) differences between the study yoga intervention and other physical activities.

Conclusions
In accordance with the accounts of participants, yoga might offer a promising intervention for breast cancer survivors. All those interviewed either currently attended a yoga class or expressed a desire to continue practicing yoga. Additionally, our findings inform future studies regarding aspects such as the importance of extending outcome measures beyond specific cancer-related complains, the advantages of addressing homogenous groups (i.e., breast cancer specific), or considering that different intervention components might need different assistance to encourage long-term use.
Restricting the level of the proteins essential for the regulation of the initiation step of replication extends the chronological lifespan and reproductive potential in budding yeast
Authors: Stępień Karolina , Enkhbaatar Tuguldur , Kula-Maximenko Monika , Jurczyk Łukasz , Skoneczna Adrianna , Mołoń Mateusz 
Published: June 2024

AbstractAging is defined as a progressive decline in physiological integrity, leading to impaired biological function, including fertility, and rising vulnerability to death. Disorders of DNA replication often lead to replication stress and are identified as factors influencing the aging rate. In this study, we aimed to reveal how the cells that lost strict control of the formation of crucial for replication initiation a pre-initiation complex impact the cells’ physiology and aging. As strains with the lower pre-IC control (lowPICC) we used, Saccharomyces cerevisiae heterozygous strains having only one functional copy of genes, encoding essential replication proteins such as Cdc6, Dbf4, Sld3, Sld7, Sld2, and Mcm10. The lowPICC strains exhibited a significant reduction in the respective genes’ mRNA levels, causing cell cycle aberrations and doubling time extensions. Additionally, the reduced expression of the lowPICC genes led to an aberrant DNA damage response, affected cellular and mitochondrial DNA content, extended the lifespan of post-mitotic cells, and increased the yeast’s reproductive potential. Importantly, we also demonstrated a strong negative correlation between the content of cellular macromolecules (RNA, proteins, lipids, polysaccharides) and aging. The data presented here will likely contribute to the future development of therapies for treating various human diseases.
Transcriptomics and metabolomics reveal hypothalamic metabolic characteristics and key genes after subarachnoid hemorrhage in rats
Authors: Liu Zongchi , Chai Zhaohui , Wu Fan , Zhang Luyuan , Wang Xiaoyi , Xu Zihan , Weng Yuxiang , Gong Jiangbiao , Shen Jian , Zhan Renya , Zhu Yu 
Published: June 2024

AbstractSubarachnoid hemorrhage (SAH) is a serious hemorrhagic event with high mortality and morbidity. Multiple injurious events produced by SAH can lead to a series of pathophysiologic processes in the hypothalamus that can severely impact patients’ life. These pathophysiologic processes usually result in physiologic derangements and dysfunction of the brain and multiple organs. This dysfunction involved multiple dimensions of the genome and metabolome. In our study, we induced the SAH model in rats to obtain hypothalamic tissue and serum. The samples were subsequently analyzed by transcriptomics and metabolomics. Next, the functional enrichment analysis of the differentially expressed genes and metabolites were performed by GO and KEGG pathway analysis. Through transcriptomic analysis of hypothalamus samples, 263 up-regulated differential genes, and 207 down-regulated differential genes were identified in SAH groups compared to Sham groups. In the KEGG pathway analysis, a large number of differential genes were found to be enriched in IL-17 signaling pathway, PI3K-Akt signaling pathway, and bile secretion. Liquid chromatography-mass spectrometry metabolomics technology was conducted on the serum of SAH rats and identified 11 up-regulated and 26 down-regulated metabolites in positive ion model, and 1 up-regulated and 10 down-regulated metabolites in negative ion model. KEGG pathways analysis showed that differentially expressed metabolites were mainly enriched in pathways of bile secretion and primary bile acid biosynthesis. We systematically depicted the neuro- and metabolism-related biomolecular changes occurring in the hypothalamus after SAH by performing transcriptomics and metabolomics studies. These biomolecular changes may provide new insights into hypothalamus-induced metabolic changes and gene expression after SAH.
Shutdown-seeking AI
Authors: Goldstein Simon , Robinson Pamela 
Published: June 2024

AbstractWe propose developing AIs whose only final goal is being shut down. We argue that this approach to AI safety has three benefits: (i) it could potentially be implemented in reinforcement learning, (ii) it avoids some dangerous instrumental convergence dynamics, and (iii) it creates trip wires for monitoring dangerous capabilities. We also argue that the proposal can overcome a key challenge raised by Soares et al. (2015), that shutdown-seeking AIs will manipulate humans into shutting them down. We conclude by comparing our approach with Soares et al.'s corrigibility framework.
CRISPR/Cas9-mediated mutagenesis of FT/TFL1 in petunia improves plant architecture and early flowering
Authors: Abdulla Mohamed Farah , Mostafa Karam , Kavas Musa 
Published: June 2024

AbstractPetunias are renowned ornamental species widely cultivated as pot plants for their aesthetic appeal both indoors and outdoors. The preference for pot plants depends on their compact growth habit and abundant flowering. While genome editing has gained significant popularity in many crop plants in addressing growth and development and abiotic and biotic stress factors, relatively less emphasis has been placed on its application in ornamental plant species. Genome editing in ornamental plants opens up possibilities for enhancing their aesthetic qualities, offering innovative opportunities for manipulating plant architecture and visual appeal through precise genetic modifications. In this study, we aimed to optimize the procedure for an efficient genome editing system in petunia plants using the highly efficient multiplexed CRISPR/Cas9 system. Specifically, we targeted a total of six genes in Petunia which are associated with plant architecture traits, two paralogous of FLOWERING LOCUS T (PhFT) and four TERMINAL FLOWER-LIKE1 (PhTFL1) paralogous genes separately in two constructs. We successfully induced homogeneous and heterogeneous indels in the targeted genes through precise genome editing, resulting in significant phenotypic alterations in petunia. Notably, the plants harboring edited PhTFL1 and PhFT exhibited a conspicuously early flowering time in comparison to the wild-type counterparts. Furthermore, mutants with alterations in the PhTFL1 demonstrated shorter internodes than wild-type, likely by downregulating the gibberellic acid pathway genes PhGAI, creating a more compact and aesthetically appealing phenotype. This study represents the first successful endeavor to produce compact petunia plants with increased flower abundance through genome editing. Our approach holds immense promise to improve economically important potting plants like petunia and serve as a potential foundation for further improvements in similar ornamental plant species.
An explainable content-based approach for recommender systems: a case study in journal recommendation for paper submission
Authors: de Campos Luis M. , Fernández-Luna Juan M. , Huete Juan F. 
Published: June 2024

AbstractExplainable artificial intelligence is becoming increasingly important in new artificial intelligence developments since it enables users to understand and consequently trust system output. In the field of recommender systems, explanation is necessary not only for such understanding and trust but also because if users understand why the system is making certain suggestions, they are more likely to consume the recommended product. This paper proposes a novel approach for explaining content-based recommender systems by specifically focusing on publication venue recommendation. In this problem, the authors of a new research paper receive recommendations about possible journals (or other publication venues) to which they could submit their article based on content similarity, while the recommender system simultaneously explains its decisions. The proposed explanation ecosystem is based on various elements that support the explanation (topics, related articles, relevant terms, etc.) and is fully integrated with the underlying recommendation model. The proposed method is evaluated through a user study in the biomedical field, where transparency, satisfaction, trust, and scrutability are assessed. The obtained results suggest that the proposed approach is effective and useful for explaining the output of the recommender system to users.
Rotenone Induces a Neuropathological Phenotype in Cholinergic-like Neurons Resembling Parkinson’s Disease Dementia (PDD)
Authors: Giraldo-Berrio Daniela , Mendivil-Perez Miguel , Velez-Pardo Carlos , Jimenez-Del-Rio Marlene 
Published: June 2024

AbstractParkinson’s disease with dementia (PDD) is a neurological disorder that clinically and neuropathologically overlaps with Parkinson’s disease (PD) and Alzheimer’s disease (AD). Although it is assumed that alpha-synuclein ($$\mathrm{\alpha }$$
α
-Syn), amyloid beta (A$$\upbeta$$
β
), and the protein Tau might synergistically induce cholinergic neuronal degeneration, presently the pathological mechanism of PDD remains unclear. Therefore, it is essential to delve into the cellular and molecular aspects of this neurological entity to identify potential targets for prevention and treatment strategies. Cholinergic-like neurons (ChLNs) were exposed to rotenone (ROT, 10$$\upmu$$
μ
M) for 24 h. ROT provokes loss of $$\Delta \mathrm{\Psi m}$$

Δ

Ψ
m

, generation of reactive oxygen species (ROS), phosphorylation of leucine-rich repeated kinase 2 (LRRK2 at Ser935) concomitantly with phosphorylation of $$\mathrm{\alpha }$$
α
-synuclein ($$\mathrm{\alpha }$$
α
-Syn, Ser129), induces accumulation of intracellular A$$\upbeta$$
β
(iA$$\upbeta$$
β
), oxidized DJ-1 (Cys106), as well as phosphorylation of TAU (Ser202/Thr205), increases the phosphorylation of c-JUN (Ser63/Ser73), and increases expression of proapoptotic proteins TP53, PUMA, and cleaved caspase 3 (CC3) in ChLNs. These neuropathological features resemble those reproduced in presenilin 1 (PSEN1) E280A ChLNs. Interestingly, anti-oxidant and anti-amyloid cannabidiol (CBD), JNK inhibitor SP600125 (SP), TP53 inhibitor pifithrin-$$\mathrm{ \alpha }$$
α
(PFT), and LRRK2 kinase inhibitor PF-06447475 (PF475) significantly diminish ROT-induced oxidative stress (OS), proteinaceous, and cell death markers in ChLNs compared to naïve ChLNs. In conclusion, ROT induces p-$$\mathrm{ \alpha }$$
α
-Syn, iA$$\upbeta$$
β
, p-Tau, and cell death in ChLNs, recapitulating the neuropathology findings in PDD. Our report provides an excellent in vitro model to test for potential therapeutic strategies against PDD. Our data suggest that ROT induces a neuropathologic phenotype in ChLNs similar to that caused by the mutation PSEN1 E280A.
Raising undergraduate researchers’ interdisciplinary consciousness through dialogue
Authors: Imbruce Valerie , Jaeger Vanessa , Rinkus Marisa A. , Hua Jessica , O’Rourke Michael 
Published: June 2024

AbstractThe importance of interdisciplinary approaches for research and education in environmental studies and sciences is well known. Integration of the multiple disciplinary approaches taught in separate courses required within these undergraduate majors and minors, however, remains a challenge. Program faculty often come from different departments and do not have time or space to integrate their own approaches with each other, resulting in individual ways of understanding interdisciplinarity. Secondly, senior capstone, thesis, or other project-based degree requirements often come too late in an undergraduate education to design an integrative project. Students would benefit from prior training in identifying complementary or divergent approaches and insights among academic specializations—a skill built from raising interdisciplinary consciousness. We present a workshop designed to enhance undergraduates’ interdisciplinary consciousness that can be easily deployed within courses or co-curricular programs, specifically summer research programs that are focused on dedicated practice within a field of study. The central question of this project is: How do we facilitate interdisciplinary consciousness and assess its impact on our students? We propose a promising, dialogue-based intervention that can be easily replicated. This dialogue would benefit academic programs like environmental studies and sciences that require the interaction and integration of discipline-based norms. We found that our dialogue intervention opens students’ perspectives on the nature of research, who research is for, epistemological differences, and the importance of practicing the research process, a unique educational experience. These perspectives are crucial to becoming collaborative, twenty-first century professionals.
Novel Nafion nanocomposite membranes embedded with TiO2-decorated MWCNTs for high-temperature/low relative humidity fuel cell systems
Authors: Nicotera Isabella , Coppola Luigi , Simari Cataldo 
Published: June 2024

AbstractExtending the operation of proton exchange membrane fuel cells (PEMFCs) at high temperature (i.e., 120 °C) and/or low relative humidity (< 50% RH) remains a significant challenge due to dehydration and subsequent performance failure of the Nafion electrolyte. We approached this problem by integrating the Nafion matrix with a novel hybrid nanofiller, created through direct growth of TiO2 nanoparticles on the surface of carbon nanotubes. This synthetic approach allowed to preserve an effective nanodispersion of Titania particles in the hosting matrix, thereby boosting dimensional stability, hydrophilicity, and physiochemical properties of the Nafion/MWCNTs-TiO2 (NMT-x) nanocomposites compared to parental Nafion. At optimal concentration (i.e., 3 wt% with respect to the polymer), the nanocomposite membrane exhibited high transport characteristics with impressive water retention capabilities, resulting in a proton conductivity of 8.3 mS cm− 1 at 80 °C and 20% RH. The Titania nanoparticles plays a key role in retaining water molecules even under dehydrating conditions, while also directly contributing to proton transport. Additionally, the long carbon nanotubes promote the formation of additional paths for proton conductivity. These combined features enabled the NMT-3 membrane to achieve a maximum power output of 307.7 mW/cm2 in a single H2/air fuel cell (5 cm2 active electrode area and 0.5 mg Pt/cm2 at both electrodes) under very challenging conditions, specifically at 120 °C and 30% RH. This represents a significant advancement towards overcoming the limitations of traditional Nafion membranes and opens up new possibilities for high-temperature, low-humidity H2/air fuel cell applications.
Non-syndromic tooth agenesis in Latvian adolescent dental patients: a retrospective study with relevant literature review
Authors: Meistere D. , Kronina L. , Karkle A. , Neimane L. 
Published: June 2024

Abstract
Aim
To investigate the prevalence of tooth agenesis and associated dental anomalies in Latvian adolescent dental patients and compare it to other European countries.

Design
Cross-sectional study of 2692 11-to-14-year-old patients (39.9% males and 60.1% females) attending Riga Stradins University Institute of Stomatology with panoramic radiographs taken between August 2020 and September 2021. Patients with any genetic syndromes were excluded. Data on tooth agenesis (excluding third molars) and other dental anomalies were recorded.

Results
The prevalence of tooth agenesis in Latvian adolescent dental patients was 9.3% with no statistically significant difference between genders (χ2 test, p = 0.472). The most commonly missing teeth were mandibular second premolars, followed by upper lateral incisors and upper second premolars. There was a statistically significant association with the presence of other dental anomalies in tooth agenesis patients (p < 0.001).

Conclusions
This study found that the prevalence of non-syndromic tooth agenesis in Latvian adolescent dental patients was 9.3% with no statistically significant differences between the genders. Patients with tooth agenesis have a statistically significant possibility of the presence of other dental anomalies (p < 0.001).