bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mesenchymal stem cells and platelet lysate in fibrin or collagen scaffold promote non‐cemented hip prosthesis integration
Authors: Dozza Barbara , Di Bella Claudia , Lucarelli Enrico , Giavaresi Gianluca , Fini Milena , Tazzari Pier Luigi , Giannini Sandro , Donati Davide 
Published: January 2011

AbstractThe objective of this study was to evaluate whether mesenchymal stem cells (MSC) and platelet lysate (PL) seeded in a fibrin or collagen scaffold could improve the new bone (NB) formation around an uncemented hip prosthesis stem in a sheep model. In vitro expanded MSC were suspended in PL and either mixed with collagen or fibrin gel as delivery vehicle. The cell–gel composites were inserted inside the femoral canal, then the prosthesis was press‐fit inserted inside the femur. Identical procedures were performed in a control group, but only the prosthesis was implanted. Histomorphometrical analysis performed 4 months after surgery indicated that the newly formed bone inside the medullary canal, between the inner cortex and the prosthetic stem, was significantly higher in the MSC–PL–collagen group (mean 18.7 ± 4.5%) and in the MSC–PL–fibrin group (mean 18.8 ± 15.2%) when compared to the control group (mean 4.6 ± 2.0%). There was a significantly higher bone–prosthesis contact in the MSC–PL–collagen group (mean 2.7 ± 2.6%) and in the MSC–PL–fibrin group (mean 2.3 ± 3.1%) compared to the control group (mean 0.2 ± 0.1%). The results indicate that MSC and PL in a fibrin or collagen scaffold can promote NB formation around an uncemented hip prosthesis stem. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29:961–968
Local application of VEGF compensates callus deficiency after acute soft tissue trauma—results using a limb‐shortening distraction procedure in rabbit tibia
Authors: Ochman Sabine , Frey Sönke , Raschke Michael J. , Deventer Jehan N. , Meffert Rainer H. 
Published: January 2011

AbstractAcute soft tissue trauma influences callus formation and fracture healing. Several studies showed a relationship between angiogenesis and bone formation during distraction osteogenesis. The purpose of this study was to investigate the influence of controlled release of vascular endothelial growth factor (VEGF) on callus formation in a limb‐shortening distraction procedure after acute compartment syndrome. Acute soft tissue trauma with critical increased compartment pressure was generated in 22 rabbits, and the limb was shortened simulating fracture site debridement. In the test group (n = 11), a VEGF‐coated collagen matrix was locally applied around the fracture, while no collagen was applied in the control group (n = 11). Following 10 days in limb shortening, a gradual distraction of 0.5 mm/12 h was performed using an external fixation device and followed up for 40 days. Osseous consolidation occurred in all animals. Average callus diameter (1.54 ± 0.8 vs. 1.27 ± 0.14 mm) and torsional strength (72% vs. 46% of normal) were significantly higher in the test versus the control group. Blood vessel formation increased with a significantly higher number of vessels (6.3 vs. 3.81/mm2) and larger cross‐sectional area (>40 µm, 90.5% vs. 86%) in the test versus control group. The results showed that locally applied VEGF stimulates fracture healing after acute soft tissue trauma and might be an option for fracture treatment in cases with severe soft tissue damage. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1093–1098, 2011
Callus patterns in femoral lengthening over an intramedullary nail
Authors: Muzaffar Nasir , Hafeez Arifa , Modi Hitesh , Song Hae‐Ryong 
Published: January 2011

AbstractOur objectives were to evaluate callus patterns seen in femoral lengthening over an intramedullary nail by Li classification regarding shape and type and to predict the result while using the nail to reduce the external fixation period and its complications. Eight hundred digital radiographs of 15 patients with 15 segments of femur shortening who underwent femoral lengthening with a monolateral external fixator over an intramedullary nail were analyzed retrospectively by four observers. Each radiograph was studied for callus shape, feature type, and callus density using pixel values. The classification was tested for concurrence and reproducibility by interobserver studies and callus patterns were compared with treatment indices to evaluate how they correlated with the outcome. Mean length gained was 4.5 cm (range: 2–8 cm). External fixator index (EFI) was 21.68 days/cm. Average distraction consolidation index (DCI) was 48.49 days/cm. Fusiform callus was seen in three cases, cylindrical in seven, and lateral in five. The homogenous pathway had higher DCI (43.7) than the heterogeneous pathway (32.9), and mixed pathways making up the rest had a DCI of 50.1. Pixel value of callus showed gradual increase in density until 20–24 weeks, then density gradually fell for 8 weeks, again increased after 32 weeks, again gradually fell, and was comparable to adjacent normal bone by 44–48 weeks. Our results suggest that the Li classification can be satisfactorily applied to lengthening procedures over intramedullary nails. The radiologic pattern and pixel value of regenerate can be correlated with the clinical outcome and can be an aid of prognostic value for the surgeon. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1106–1113, 2011
Diffuse malignant peritoneal mesothelioma: Systematic review of clinical management and biological research
Authors: Baratti Dario , Kusamura Shigeki , Deraco Marcello 
Published: January 2011

AbstractDiffuse malignant peritoneal mesothelioma (DMPM) is an uncommon and locally aggressive tumor with poor prognosis. Currently, no standard therapy is available. The biology of this disease is still poorly understood. We performed a systematic search of relevant studies on clinical management and biological research of DMPM. Trials were selected using a predetermined protocol. The current evidence suggests that cytoreductive surgery (CRS) and perioperative intraperitoneal chemotherapy (PIC) results in improved survival. Biological understanding of DMPM is currently evolving. J. Surg. Oncol. 2011;103:822–831. © 2011 Wiley‐Liss, Inc.
The concepts in poorly differentiated carcinoma of the thyroid: A review article
Authors: Nambiar Ajit , Pv Sunil , Susheelan Vijaylakshmi , Kuriakose Moni Abraham 
Published: January 2011

AbstractPoorly differentiated carcinomas of the thyroid have evolved over the past half century into a specific entity in the classification of thyroid carcinomas. The need for a consensus diagnostic criteria has been stressed in view of the wide discrepancies and confusion among pathologists and clinicians. This review attempts to understand the concept, the evolution, clarity and validity of the concepts in poorly differentiated carcinomas of the thyroid. J. Surg. Oncol. 2011;103:818–821. © 2011 Wiley‐Liss, Inc.
Assessment of lymphatic drainage patterns and implications for the extent of neck dissection in head and neck melanoma patients
Authors: Klop W. Martin C. , Veenstra Hidde J. , Vermeeren Lenka , Nieweg Omgo E. , Balm Alfons J.M. , Lohuis Peter J.F.M. 
Published: January 2011

AbstractBackground and ObjectivesThe aim of this study is to evaluate lymphatic drainage with sentinel node location data in patients with head and neck cutaneous melanoma, and to determine the implications for the extent of therapeutic neck dissections.MethodsSixty‐five patients with head and neck cutaneous melanoma without evidence of regional metastases at ultrasound guided fine needle aspiration cytology examination were included. Lymphatic drainage patterns were investigated using planar and dynamic lymphoscintigraphy, and SPECT/CT. Biopsy of sentinel nodes was guided by images and gamma probe. The incidence of discordant sentinel nodes was determined by comparing actual drainage patterns to “O'Briens map” and to the treatment guidelines of The Netherlands Cancer Institute.ResultsSentinel node identification was successful in 98% of the patients. Fifteen patients (23%) were diagnosed with a tumor‐positive sentinel node. Two sentinel node‐negative patients (3%) developed a regional lymph node metastasis (false‐negative ratio: 12%). Twenty‐three percent of the harvested sentinel nodes were discordant according to “O'Brien's map,” while 14% were discordant according to the treatment guidelines of The Netherlands Cancer Institute (P < 0.001).ConclusionsAlmost a quarter of head and neck melanomas metastasize outside clinically predicted neck levels. Neck surgery guidelines of The Netherlands Cancer Institute provide for a smaller number of discordant sentinel nodes. J. Surg. Oncol. 2011;103:756–760. © 2011 Wiley‐Liss, Inc.
Carcinoid tumors of the appendix: A population‐based study
Authors: Mullen John T. , Savarese Diane M.F. 
Published: February 2011

AbstractBackgroundCarcinoid tumors of the appendix are rare, and as such there are few data guiding their optimal treatment.MethodsThe analysis included all patients with malignant, typical carcinoid tumor of the appendix for whom complete data were available in the Surveillance, Epidemiology, and End Results database between 1988 and 2003. Clinicopathologic factors predicting lymph node (LN) involvement and survival were determined.ResultsLN metastases were present in 44 of 89 patients (49%), including 4 of 27 patients (15%) with tumors ≤ 1.0 cm, 16 of 34 patients (47%) with tumors > 1.0 cm but ≤ 2.0 cm, and 24 of 28 patients (86%) with tumors >2.0 cm. Increasing tumor size predicted LN involvement, whereas age, gender, and depth of tumor invasion did not. Excluding patients with distant metastasis (DM), the 10‐year overall survival rates of patients with positive LNs and tumor sizes ≤1.0 cm, >1.0 cm but ≤2.0 cm, and >2.0 cm were 100%, 92%, and 91%, respectively.ConclusionsRight hemicolectomy should be considered for patients with appendiceal carcinoid tumors >1.0 cm in size given their high incidence of LN metastases and limited data concerning the natural history of unresected LN metastases. J. Surg. Oncol. 2011;104:41–44. © 2011 Wiley‐Liss, Inc.
Reversed‐phase liquid chromatographic separation of antiretroviral drugs on a monolithic column using ionic liquids as mobile phase additives
Authors: Nageswara Rao Ramisetti , Ramachandra Bondigalla , Mastan Vali Rajavarapu 
Published: January 2011

AbstractThe use of 3‐methylimidazolium cation‐based ionic liquids (ILs) was evaluated as mobile phase additives for separation of antiretroviral drugs on a monolithic column by RP‐HPLC. Separation of eight commonly used antiretroviral drugs was achieved on a Chromolith Flash, RP‐18e column (25×4.6 mm, porous material) using water (pH 4.0 adjusted with acetic acid)/methanol v/v as a mobile phase containing ILs in a gradient elution mode. The effects of concentrations of ILs on retention, resolution and peak shape were studied and a regression equation correlating the interactions between stationary phase and the ILs was established. The retention of all the drugs was decreased notably by using 1‐butyl‐3‐methylimidazolium tetrafluoroborate, while 1‐ethyl‐3‐methylimidazolium methylsulfate reduced gradient drift drastically when compared to triethylamine.
Preparation and chiral recognition in HPLC of cellulose 3,5‐dichlorophenylcarbamates immobilized onto silica gel
Authors: Qu Hai‐tao , Li Jun‐qing , Wu Guang‐shun , Shen Jun , Shen Xian‐de , Okamoto Yoshio 
Published: January 2011

AbstractThe 3,5‐dichlorophenylcarbamates (2) of cellulose bearing a small amount of 3‐(triethoxysilyl)propyl residues were synthesized by a one‐pot process and immobilized onto a silica gel through intermolecular polycondensation of the triethoxysilyl groups. The obtained cellulose derivatives were characterized by 1H NMR and elemental analysis (EA), and their recognition abilities were evaluated by high‐performance liquid chromatography (HPLC). The cellulose derivatives containing about 1–5% of the 3‐(triethoxysilyl)propyl residue were efficiently immobilized with a high chiral recognition ability. The immobilized chiral packing materials (CPMs) could be used with the eluents containing chloroform and tetrahydrofuran (THF), which cannot be used with the conventional coated‐type chiral packing materials. By using these eluents, the chiral recognition for many racemates was improved.
Determination of nitroanilines in hair dye using polymer monolith microextraction coupled with HPLC
Authors: Xiao Pengfei , Bao Changli , Jia Qiong , Su Riyan , Zhou Weihong , Jia Jianbo 
Published: January 2011

AbstractIn this study, a novel method for the determination of nitroanilines in hair dye samples has been developed based on poly(methacrylic acid‐co‐ethylene glycol dimethacrylate) monolith microextraction (PMME) and high‐performance liquid chromatography (HPLC) analysis. Four nitroanilines, p‐nitroaniline (PNAL), m‐nitroaniline (MNAL), o‐nitroaniline (ONAL), and 2,4‐dinitroaniline (DNAL), are studied as representatives. To obtain optimum extraction efficiency, several experimental parameters including sample flow rate, sample volume, sample pH, and eluent flow rate have been investigated. Under the optimal experimental conditions, the linear regression coefficients of the standard curves are greater than 0.9990. The limits of detection for p‐nitroaniline, m‐nitroaniline, o‐nitroaniline, and 2,4‐dinitroaniline are 0.012, 0.008, 0.018, and 0.005 μg/mL, respectively. The intraday and interday relative standard deviations are less than 3.1 and 5.4%, respectively. The proposed method is simple, rapid, sensitive, and competent when used for the determination of nitroanilines in hair dye samples and the accuracy is assessed through recovery experiments.
Poly(arylene ether)s with Pendant Perfluoroalkyl Sulfonic Acid Groups as Proton‐Exchange Membrane Materials
Authors: Ghassemi Hossein , Schiraldi David A. , Zawodzinski Thomas A. , Hamrock Steven 
Published: January 2011

AbstractNew membranes were prepared and evaluated as polymer electrolytes for PEMFCs. Polymers with PSU in the main chain and –C(O)(CF2)3SO3H in the side chain (PSU‐PSA) were synthesized by substitution of PSU‐Li and SFBF, followed by treatment with aqueous potassium hydroxide then sulfuric acid. The proton conductivity of PSU‐PSA with an ion exchange capacity (IEC) of 1.12 mmol · g−1 was 0.091 S · cm−1 at 80 °C under 100% relative humidity. A hydrogen/air PEMFC test of PSU‐PSA with an IEC value of 1.12 mmol · g−1 produced a maximum power output of 0.265 W · cm−2. A DMA measurement revealed that PSU‐PSA had an α‐relaxation temperature around 196 °C which is higher than the value reported for Nafion. magnified image
Poly(meth)acrylates Obtained by Cascade Reaction
Authors: Popescu Dragos , Keul Helmut , Moeller Martin 
Published: January 2011

AbstractPreparation, purification, and stabilization of functional (meth)acrylates with a high dipole moment are complex, laborious, and expensive processes. In order to avoid purification and stabilization of the highly reactive functional monomers, a concept of cascade reactions was developed comprising enzymatic monomer synthesis and radical polymerization. Transacylation of methyl acrylate (MA) and methyl methacrylate (MMA) with different functional alcohols, diols, and triols (1,2,6‐hexanetriol and glycerol) in the presence of Novozyme 435 led to functional (meth)acrylates. After the removal of the enzyme by means of filtration, removal of excess (meth)acrylate and/or addition of a new monomer, e.g., 2‐hydroxyethyl (meth)acrylate the (co)polymerization via free radical (FRP) or nitroxide mediated radical polymerization (NMP) resulted in poly[(meth)acrylate]s with predefined functionalities. Hydrophilic, hydrophobic as well as ionic repeating units were assembled within the copolymer. The transacylation of MA and MMA with diols and triols carried out under mild conditions is an easy and rapid process and is suitable for the preparation of sensitive monomers. magnified image
Association of olfactory dysfunction and brain. Metabolism in Parkinson's disease
Authors: Baba Toru , Takeda Atsushi , Kikuchi Akio , Nishio Yoshiyuki , Hosokai Yoshiyuki , Hirayama Kazumi , Hasegawa Takafumi , Sugeno Naoto , Suzuki Kyoko , Mori Etsuro , Takahashi Shoki , Fukuda Hiroshi , Itoyama Yasuto 
Published: January 2011

AbstractHyposmia is one of the cardinal early symptoms of Parkinson disease (PD). Accumulating clinical and pathological evidence suggests that dysfunction of the olfactory‐related cortices may be responsible for the impaired olfactory processing observed in PD; however, there are no clear data showing a direct association between altered brain metabolism and hyposmia in PD. In this study, we evaluated brain glucose metabolism and smell‐identification ability in 69 Japanese patients with nondemented PD. Olfactory function was assessed using the Odor Stick Identification Test for Japanese. The regional cerebral metabolic rate of glucose consumption at rest was measured using 18F‐fluorodeoxyglucose positron emission tomography and was analyzed using SPM‐based group comparisons and the brain–behavior partial least‐squares method. We found that olfactory dysfunction was closely related to cognitive dysfunction, including memory impairment. Moreover, brain–behavior partial least‐squares analysis revealed that odor‐identification performance was closely associated with broad cortical dysfunction, including dysfunction of the piriform cortex and amygdala. Our results suggest that the cognitive deficit in olfactory perception is an important aspect of hyposmia in PD and that this deficit is caused by altered brain metabolism in the amygdala and piriform cortex. © 2011 Movement Disorder Society
Vitamin D intestinal absorption is not a simple passive diffusion: Evidences for involvement of cholesterol transporters
Authors: Reboul Emmanuelle , Goncalves Aurélie , Comera Christine , Bott Romain , Nowicki Marion , Landrier Jean‐François , Jourdheuil‐Rahmani Dominique , Dufour Claire , Collet Xavier , Borel Patrick 
Published: January 2011

AbstractScope: It is assumed that vitamin D is absorbed by passive diffusion. However, since cholecalciferol (vitamin D3) and cholesterol display similar structures, we hypothesized that common absorption pathways may exist.Methods and results: Cholecalciferol apical transport was first examined in human Caco‐2 and transfected Human embryonic kidney (HEK) cells. Cholecalciferol uptake was then valuated ex vivo and in vivo, using either wild‐type mice, mice overexpressing Scavenger Receptor class B type I (SR‐BI) at the intestinal level or mice treated or not with ezetimibe. Cholecalciferol uptake was concentration‐, temperature‐ and direction‐dependent, and was significantly impaired by a co‐incubation with cholesterol or tocopherol in Caco‐2 cells. Moreover Block Lipid Transport‐1 (SR‐BI inhibitor) and ezetimibe glucuronide (Niemann‐Pick C1 Like 1 inhibitor) significantly decreased cholecalciferol transport. Transfection of HEK cells with SR‐BI, Cluster Determinant 36 and Niemann‐Pick C1 Like 1 significantly enhanced vitamin D uptake, which was significantly decreased by the addition of Block Lipid Transport‐1, sulfo‐N‐succinimidyl oleate (Cluster Determinant 36 inhibitor) or ezetimibe glucuronide, respectively. Similar results were obtained in mouse intestinal explants. In vivo, cholecalciferol uptake in proximal intestinal fragments was 60% higher in mice overexpressing SR‐BI than in wild‐type mice (p<0.05), while ezetimibe effect remained non‐significant.Conclusion: These data show for the first time that vitamin D intestinal absorption is not passive only but involves, at least partly, some cholesterol transporters.
Three‐Dimensional MR image registration of the human brain
Authors: Bedell Barry J. , Narayana Ponnada A. , Johnston Dennis A. 
Published: February 2011

AbstractAn automatic three‐dimensional technique for registration of MR images of human brain is described. The algorithm was tested, using MR images of human brain, and was found to estimate angular offsets to within 0.5° and translational offsets to within about 1 pixel. The quality of final registration was evaluated by histogram analysis. The algorithm was found to be computationally efficient and robust.
Localized 1H NMR spectroscopy of rat spinal cord in Vivo
Authors: Zelaya Fernando O. , Chalk Jonathan B. , Mullins Paul , Brereton Ian M. , Doddrell David M. 
Published: February 2011

AbstractA movable, actively decoupled surface coil has been employed to obtain a localized 1H NMR spectrum from the lumbosacral spinal cord of a live Lewis rat. A volume selective ‘VOSY’ normally spelled out as ‘volume selective spectroscopy’ spectroscopy pulse sequence that incorporates “phase ramped” selective RF pulses, has been used to minimize random phase jitter in the NMR signal as a result of the large frequency shifts required to locate the voxel in the center of the cord while using intense gradient pulses. Spectra from 13‐μl voxels in healthy rats and in rats inoculated with guinea pig spinal cord and complete Freund's adjuvant, resulting in experimental autoimmune encephalomyelitis, are shown.
Cerebrovascular changes in rats during ischemia and reperfusion: A comparison of bold and first pass bolus tracking techniques
Authors: Jones Richard A. , Müller Tomm B. , Haraldseth Olav , Baptista António M. , Øksendal Audun N. 
Published: February 2011

AbstractBoth first pass bolus tracking of a susceptibility contrast agent and blood oxygenation level dependent (BOLD) sequences provide information on the tissue perfusion and the cerebral blood volume, but each sequence has its own particular limitations. In this article, both techniques were used to assess the cerebrovascular changes occurring in a rat model of focal cerebral ischemia with reperfusion after 2 h of ischemia. The blood oxygenation level dependent studies were performed before, during, and after 60 s of anoxia to observe the response of the tissue to a respiratory challenge. Both techniques were able to detect ischemia and reperfusion; however, first pass bolus tracking provided better sensitivity and was easier to interpret. Because the blood oxygenation level dependent sequence did not provide any additional information, bolus tracking would appear to be the method of choice for studies of cerebral ischemia with reperfusion.
Ferritin effect on the transverse relaxation of water: NMR microscopy at 9.4 T
Authors: Gottesfeld Ziv , Neeman Michal 
Published: February 2011

AbstractAccumulation of ferritin, the iron storage protein, has been linked recently to aging and a number of pathologies. Noninvasive detection of iron storage by MRI relies on its extremely strong effect on water relaxation. The aim of this article is to characterize the effect of ferritin on transverse water relaxation in a high magnetic field, using an imaging Carr‐Purcell Meiboom‐Gill (CPMG) preparation sequence. Ferritin‐induced water relaxation showed quadratic dependence on the iron loading factor, implying a paramagnetic mechanism. However, an additional zero order term was found, that could be due to the initial stages of the iron core loading. Significant enhancement of ferritin contrast was obtained at very short τCPMG durations. This approach for enhancing ferritin contrast was demonstrated by NMR microscopy of ferritin‐injected Xenopus oocytes, thus showing the feasibility of ferritin detection in a high magnetic field, even in systems with short transverse relaxation.