bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cross-talk of MLST and transcriptome unveiling antibiotic resistance mechanism of carbapenem resistance Acinetobacter baumannii clinical strains isolated in Guiyang, China
Authors: Qiu Zhilang , Yuan Kexin , Cao Huijun , Chen Sufang , Chen Feifei , Mo Fei , Guo Guo , Peng Jian 
Published: June 2024

IntroductionAcinetobacter baumannii (A. baumannii) is an important opportunistic pathogen causing nosocomial infection in the clinic. The occurrence rate of antibiotic resistance is increasing year by year, resulting in a highly serious situation of bacterial resistance.MethodsTo better understand the local epidemiology of multidrug-resistant A. baumannii, an investigation was conducted on the antibiotic resistance of different types of A. baumannii and its relationship with the genes of A. baumannii. Furthermore, the molecular mechanism underlying antibiotic resistance in A. baumannii was investigated through transcriptome analysis.ResultsThese results showed that a total of 9 STs were detected. It was found that 99% of the strains isolated in the hospital belonged to the same STs, and the clone complex CC208 was widely distributed in various departments and all kinds of samples. Furthermore, these A. baumannii strains showed high resistance to ertapenem, biapenem, meropenem, and imipenem, among which the resistance to ertapenem was the strongest. The detection rate of blaOXA–51 gene in these carbapenem resistance A. baumannii (CRAB) reached 100%; Additionally, the transcriptome results showed that the resistance genes were up-regulated in resistance strains, and these genes involved in biofilm formation, efflux pumps, peptidoglycan biosynthesis, and chaperonin synthesis.DiscussionThese results suggest that the CC208 STs were the main clonal complex, and showed high carbapenem antibiotic resistance. All these resistant strains were distributed in various departments, but most of them were distributed in intensive care units (ICU). The blaOXA–23 was the main antibiotic resistance genotype; In summary, the epidemic trend of clinical A. baumannii in Guiyang, China was analyzed from the molecular level, and the resistance mechanism of A. baumannii to carbapenem antibiotics was analyzed with transcriptome, which provided a theoretical basis for better control of A. baumannii.
Salinity affects microbial function genes related to nutrient cycling in arid regions
Authors: Li Yan , Li Wenjing , Jiang Lamei , Li Eryang , Yang Xiaodong , Yang Jianjun 
Published: June 2024

IntroductionSalinization damages soil system health and influences microbial communities structure and function. The response of microbial functions involved in the nutrient cycle to soil salinization is a valuable scientific question. However, our knowledge of the microbial metabolism functions in salinized soil and their response to salinity in arid desert environments is inadequate.MethodsHere, we applied metagenomics technology to investigate the response of microbial carbon (C), nitrogen (N), phosphorus (P), and sulfur (S) cycling and the key genes to salinity, and discuss the effects of edaphic variables on microbial functions.ResultsWe found that carbon fixation dominated the carbon cycle. Nitrogen fixation, denitrification, assimilatory nitrate reduction (ANRA), and nitrogen degradation were commonly identified as the most abundant processes in the nitrogen cycle. Organic phosphorus dissolution and phosphorus absorption/transport were the most enriched P metabolic functions, while sulfur metabolism was dominated by assimilatory sulfate reduction (ASR), organic sulfur transformation, and linkages between inorganic and organic sulfur transformation. Increasing salinity inhibited carbon degradation, nitrogen fixation, nitrogen degradation, anammox, ANRA, phosphorus absorption and transport, and the majority of processes in sulfur metabolism. However, some of the metabolic pathway and key genes showed a positive response to salinization, such as carbon fixation (facA, pccA, korAB), denitrification (narG, nirK, norBC, nosZ), ANRA (nasA, nirA), and organic phosphorus dissolution processes (pstABCS, phnCD, ugpAB). High salinity reduced the network complexity in the soil communities. Even so, the saline microbial community presented highly cooperative interactions. The soil water content had significantly correlations with C metabolic genes. The SOC, N, and P contents were significantly correlated with C, N, P, and S network complexity and functional genes. AP, NH4+, and NO3− directly promote carbon fixation, denitrification, nitrogen degradation, organic P solubilization and mineralization, P uptake and transport, ASR, and organic sulfur transformation processes.ConclusionSoil salinity in arid region inhibited multiple metabolic functions, but prompted the function of carbon fixation, denitrification, ANRA, and organic phosphorus dissolution. Soil salinity was the most important factor driving microbial functions, and nutrient availability also played important roles in regulating nutrient cycling.
Tetrandrine inhibits aldosterone synthesis by covalently targeting CYP11A1 to attenuate hypertension
Authors: Chu Simeng , Yang Wei , Lu Yujie , Li Junjie , Peng Jiamin , Liu Wenjuan , Jiang Min , Bai Gang 
Published: June 2024

IntroductionTetrandrine (Tet) is the main pharmacological component of Stephania tetrandra S. Moore, which is a well-documented traditional Chinese medicine known for its diuretic and antihypertensive properties. Unraveling the specific targets and mechanisms of Tet involved in inducing diuresis and mitigating hypertension can provide valuable insights into its therapeutic effects. This study aimed to explore the diuretic and antihypertensive targets and mechanisms of Tet using chemical biology coupled with activity analyses in vivo and in vitro.MethodsThe diuretic effects of Tet were evaluated using a water-loaded mouse model. The direct target proteins for the diuretic and antihypertensive effects of Tet were determined using chemical biology. Furthermore, the molecular mechanism of Tet binding to target proteins was analyzed using a multidisciplinary approach based on the structure and function of the proteins. Finally, the effects of the Tet-targeted protein on downstream signaling pathways and blood pressure were evaluated in hypertensive model rats.ResultsTet exhibited significant antihypertensive and potassium-preserving diuretic effects. The mechanism underlying these effects involves the modulation of the enzyme activity by covalent binding of Tet to Cys423 of CYP11A1. This interaction alters the stability of heme within CYP11A1, subsequently impeding electron transfer and inhibiting aldosterone biosynthesis.DiscussionThis study not only revealed the mechanism of the diuretic and antihypertensive effects of Tet but also discovered a novel covalent inhibitor of CYP11A1. These findings contribute significantly to our understanding of the therapeutic potential of Tet and provide a foundation for future research in the development of targeted treatments for hypertension.
Capecitabine-associated gastrointestinal ulceration, haemorrhage, and obstruction: a pharmacovigilance analysis based on the FAERS
Authors: Wang Yuwei , Meng Long , Liu Xiao 
Published: June 2024

BackgroundCapecitabine has been reported to be associated with severe gastrointestinal (GI) adverse drug reactions (gastrointestinal ulceration, haemorrhage, and obstruction). However, statistical correlations have not been demonstrated, and specific GI adverse drug reactions, such as GI obstruction, are not listed on its label.AimWe aimed to determine the associations between capecitabine and GI ulceration, haemorrhage, or obstruction among patients with breast cancer by examining data from the United States Food and Drug Administration Adverse Event Reporting System (FAERS).MethodsWe performed disproportionality analysis of GI ulceration, haemorrhage, and obstruction by evaluating the reporting odds ratio (ROR) and the information component (IC) with their 95% confidence intervals (CIs).ResultsWe identified 279 patients with capecitabine-associated GI ulceration, haemorrhage, or obstruction reported between 1 January 2004 and 31 December 2020. One-fourth of the cases of GI ulceration, haemorrhage, or obstruction resulted in death. Capecitabine as a drug class had disproportionately high reporting rates for GI ulceration [ROR 1.94 (1.71–2.21); IC 0.80 (0.60–0.99)], haemorrhage [ROR 2.27 (1.86–2.76); IC 0.99 (0.69–1.28)], and obstruction [ROR 2.19 (1.63–2.95); IC 0.96 (0.51–1.40)].ConclusionPharmacovigilance research on the FAERS has revealed a slight increase in reports of GI ulceration, haemorrhage, and obstruction in capecitabine users, which may cause serious or deadly consequences. In addition to the adverse reactions described in the package insert, close attention should be paid to GI obstruction to avoid discontinuation or life-threatening outcomes.
A causal relationship between particulate matter 2.5 and obesity and its related indicators: a Mendelian randomization study of European ancestry
Authors: Wu Tian qiang , Han Xinyu , Liu Chun yan , Zhao Na , Ma Jian 
Published: June 2024

BackgroundIn recent years, the prevalence of obesity has continued to increase as a global health concern. Numerous epidemiological studies have confirmed the long-term effects of exposure to ambient air pollutant particulate matter 2.5 (PM2.5) on obesity, but their relationship remains ambiguous.MethodsUtilizing large-scale publicly available genome-wide association studies (GWAS), we conducted univariate and multivariate Mendelian randomization (MR) analyses to assess the causal effect of PM2.5 exposure on obesity and its related indicators. The primary outcome given for both univariate MR (UVMR) and multivariate MR (MVMR) is the estimation utilizing the inverse variance weighted (IVW) method. The weighted median, MR-Egger, and maximum likelihood techniques were employed for UVMR, while the MVMR-Lasso method was applied for MVMR in the supplementary analyses. In addition, we conducted a series of thorough sensitivity studies to determine the accuracy of our MR findings.ResultsThe UVMR analysis demonstrated a significant association between PM2.5 exposure and an increased risk of obesity, as indicated by the IVW model (odds ratio [OR]: 6.427; 95% confidence interval [CI]: 1.881–21.968; PFDR = 0.005). Additionally, PM2.5 concentrations were positively associated with fat distribution metrics, including visceral adipose tissue (VAT) (OR: 1.861; 95% CI: 1.244–2.776; PFDR = 0.004), particularly pancreatic fat (OR: 3.499; 95% CI: 2.092–5.855; PFDR =1.28E-05), and abdominal subcutaneous adipose tissue (ASAT) volume (OR: 1.773; 95% CI: 1.106–2.841; PFDR = 0.019). Furthermore, PM2.5 exposure correlated positively with markers of glucose and lipid metabolism, specifically triglycerides (TG) (OR: 19.959; 95% CI: 1.269–3.022; PFDR = 0.004) and glycated hemoglobin (HbA1c) (OR: 2.462; 95% CI: 1.34–4.649; PFDR = 0.007). Finally, a significant negative association was observed between PM2.5 concentrations and levels of the novel obesity-related biomarker fibroblast growth factor 21 (FGF-21) (OR: 0.148; 95% CI: 0.025–0.89; PFDR = 0.037). After adjusting for confounding factors, including external smoke exposure, physical activity, educational attainment (EA), participation in sports clubs or gym leisure activities, and Townsend deprivation index at recruitment (TDI), the MVMR analysis revealed that PM2.5 levels maintained significant associations with pancreatic fat, HbA1c, and FGF-21.ConclusionOur MR study demonstrates conclusively that higher PM2.5 concentrations are associated with an increased risk of obesity-related indicators such as pancreatic fat content, HbA1c, and FGF-21. The potential mechanisms require additional investigation.
A study on the falls factors among the older adult with cognitive impairment based on large-sample data
Authors: Wang Changying , Zhang Yunwei , Wang Jin , Wan Lingshan , Li Bo , Ding Hansheng 
Published: June 2024

IntroductionThis study explored the correlative factors of falls among the older adult with cognitive impairment, to provide distinct evidence for preventing falls in the older adult with cognitive impairment compared with the general older adult population.MethodsThis study was based on a cross-sectional survey, with an older adult population of 124,124 was included. The data was sourced from the Elderly Care Unified Needs Assessment for Long-Term Care Insurance in Shanghai. Binary and multivariable logistic regression analyses were conducted sequentially on the correlative factors of falls. Multivariable logistic regression was performed on variables that were significant, stratified by cognitive function levels.ResultsThe incidence of fall in the past 90 days was 17.67% in this study. Specific variables such as gender (male), advanced age (≥80), residence with a elevator (or lift), mild or moderate disability, quality of sleep (acceptable/poor) were negatively correlated with falls, while higher education level, living alone, residence with indoor steps, unclean and untidy living environment, MCI or dementia, chronic diseases, restricted joints, impaired vision, and the use of diaper were positively correlative factors of falls. Comparing with older adult with normal cognitive functions, older adult with dementia faced a higher risk of falling due to accessibility barrier in the residence. For general older adults, less frequency of going outside and poor social interactions were positively correlated with falls, while for older adult with cognitive impairments, going outside moderately (sometimes) was found positively correlated with falls. Older adults with cognitive impairments have increased fall risks associated with chronic diseases, restricted joints, and the use of diaper. The risk of falling escalated with the greater number of chronic diseases.DiscussionFor older adult with cognitive impairments, it is advisable to live with others. Additionally, creating an accessible living environment and maintaining the cleanness and tidiness can effectively reduce the risk of falls, particularly for those with MCI or dementia. Optimal outdoor activity plans should be developed separately based on the cognitive function of older adults. Older adult with dementia who have comorbidities should be paid special attention in fall prevention compared to the general older adult population.
Implications of intracochlear decomposition gas formation in non-putrefied cadavers
Authors: Mittmann Philipp , Ernst Arne , Seidl Rainer , Lauer Gina , Gölz Leonie , Mutze Sven , Windgassen Marc , Buschmann Claas 
Published: June 2024

IntroductionPostmortem computed tomography (pmCT) prior to forensic autopsy has become increasingly important in recent decades, especially in forensic documentation of single injuries, injury patterns, and causes of death. Postmortem decomposition gas formation can also be detected in pmCT scans, which might affect cochlear implant research in postmortem human temporal bones (TBs).Material and methodsFifty non-putrefied hanging fatalities within a 2-year period (January 2017 to December 2019) were included with 100 TBs. Each body underwent whole-body pmCT prior to forensic autopsy. PmCT scans were analyzed with respect to the presence of intracochlear gas despite the lack of putrefaction at autopsy by an experienced fellow neurotologist.ResultsPmCT revealed gas formation in two individuals despite the lack of head trauma and putrefaction at postmortem examination and autopsy. Both individuals showed enclosed gas in the vestibule and the cochlea on both sides.DiscussionIntracochlear gas formation, most likely related to decomposition, may occur despite the lack of putrefaction at postmortem examination and autopsy and can be detected by pmCT. This finding seems to be rather rare in non-traumatic death cases but might affect cochlear pressure research in postmortem human TB.
The Impact of Air Pollution Controls on Health and Health Inequity Among Middle-Aged and Older Chinese: Evidence From Panel Data
Authors: Zhao Yaxin , Peng Zixuan , Zhou Zhongliang , Zhai Xiaohui , Gong Shaoqing , Shen Chi , Zhang Tianci , Zhao Dantong , Cao Dan 
Published: June 2024

ObjectivesWe evaluated the long-term effects of air pollution controls on health and health inequity among Chinese &gt;45 years of age.MethodsData were derived from the China Health Aging and Retirement Longitudinal Survey and the China National Environmental Monitoring Centre. Decreases in PM2.5 and PM10 were scaled to measure air quality controls. We used a quasi-experimental design to estimate the impact of air quality controls on self-reported health and health inequity. Health disparities were estimated using the concentration index and the horizontal index.ResultsAir pollution controls significantly improved self-reported health by 20% (OR 1.20, 95% CI, 1.02–1.42). The poorest group had a 40% (OR 1.41, 95% CI, 0.96–2.08) higher probability of having excellent self-reported health after air pollution controls. A pro-rich health inequity was observed, and the horizontal index decreased after air pollution controls.ConclusionAir pollution controls have a long-term positive effect on health and health equity. The poorest population are the main beneficiaries of air pollution controls, which suggests policymakers should make efforts to reduce health inequity in air pollution controls.
L’aire d’ensilage de Saint-Pastour à Vergèze (Gard), VIIIe-XIIe siècles : témoin de l’exploitation du terroir et de la proximité d’un habitat groupé
Authors: Rochette Marie , Chardenon Nathalie , Commandré Isabelle , Hulin Guillaume , Ott Mathieu 
Published: June 2024

En 2014 se prescribió el estudio de catorce hectáreas donde se sitúa el yacimiento de Saint-Pastour, al sur de la comuna de Vergèze en el departamento del Gard, en el marco de la creación de canteras para las obras de la línea de alta velocidad entre Nîmes y Montpellier.
Dentro de esta área se excavaron seis zonas de algo más de tres hectáreas en total alrededor de los principales vestigios detectados durante el diagnóstico (Tosna, Bouchet 2013). Estos restos se adscriben a varios períodos cronológicos y atestiguan las diferentes fases de frecuentación u ocupación de este sector de la llanura. La operación fue coordinada por Jean-Yves Breuil y la dirección científica estuvo a cargo de Pierre Séjalon. Aunque los vestigios medievales están presentes en el conjunto de la excavación, es en la zona más meridional situada al norte de la masada de Saint-Pastour donde la ocupación es más densa, ya que allí se localizó una zona de ensilado colectivo de los siglos VIII al XII.
EVALUACIÓN in vitro DE LA ACTIVIDAD CICATRIZANTE Y ANTIBACTERIANA DE EXTRACTOS DE Buddleja cordata KUNTH Y Vismia baccifera (L.) TRIANA & PLANCH
Authors: Hernández-Pasteur Griselda , Silva-Bermúdez Phaedra S. , Reyes-Chilpa Ricardo , Vibrans Heike , Soto-Hernández Marcos 
Published: June 2024

La piel es el órgano más grande del cuerpo humano y cumple diversas funciones como barrera de protección. Ésta puede verse afectada por lesiones que interrumpen la continuidad de su estructura; y por infecciones microbianas que retardan el proceso de cicatrización. En la medicina tradicional se usan plantas para el tratamiento de heridas mediante infusiones, decocciones o cataplasmas. Dos especies distribuidas en México que tienen reportes etnomédicos para dicho fin son Buddleja cordata Kunth (tepozán) y Vismia baccifera (L.) Triana & Planch (chotillo). El objetivo de este trabajo fue evaluar in vitro el potencial de estas especies para promover el cierre de heridas y como agentes antibacterianos contra Pseudomonas aeruginosa.
Los extractos orgánicos de las hojas de ambas especies se evaluaron en un modelo in vitro de herida en monocapa celular y en un modelo de microdilución contra P. aeruginosa. También se evaluó in vitro el posible efecto citotóxico de los extractos mediante ensayo de reducción de la sal de tetrazolio. El extracto de diclorometano-metanol de tepozán a 50 μg mL-1 incrementó significativamente (35 %) el cierre de herida en monocapa celular. El efecto puede deberse al verbascosido, compuesto con actividad cicatrizante previamente identificado en extractos polares de hojas de tepozán. El extracto de V. baccifera no incrementó la tasa de reparación en el modelo de herida in vitro usado. Ninguno de los extractos evaluados en las dos especies presentó efecto citotóxico (hasta 100 μg mL-1); tampoco se inhibió el crecimiento de P. aeruginosa (hasta 1000 μg mL-1). Los resultados sugieren que el uso popular de tepozán para el tratamiento de heridas tiene sustento científico, pero aún se requiere profundizar en su estudio químico y farmacológico in vivo.
Concepto de colonia cerrada, alcances y limitaciones desde una perspectiva de legalidad y de constitucionalidad
Authors: Yescas Figueroa Gonzalo , Olivas Almada Jesús Eduardo 
Published: June 2024

Exponen la legalidad y la constitucionalidad del concepto de barrio o colonia cerrada, evidencian las acciones tomadas por quienes presiden las mesas directivas de las colonias con una justificación basada en cuestiones de seguridad pública y los alcances jurídicos de dichos actos que originan violaciones de derechos fundamentales y tensiones entre vecinos. Delimita la dimensión normativa del derecho administrativo y constitucional con los conceptos de eficacia y validez y confronta las teorías extra garantistas de los ciudadanos
المجتمع الليبي في مواجهة الاحتلال والاضطهاد الروماني والبيزنطي بين (الوحدة الوطنية ووحدة المعتقد )
Authors: محمد سعيد غريدة د. سعيد 
Published: June 2024

يتعلق موضوع الدراسة بفترة من الفترات المهمة في تاريخ ليبيا العصر الوسيط , وخاصة فترة تسرب وانتشار المسيحية إلى الشمال الأفريقي بشكل عام وإلى ليبيا بشكل خاص , زمن الاحتلال الروماني والبيزنطي , كديانة سماوية وجديدة في القرون الأولى الميلادية ، تسلط هذه الدراسة الضوء على مرحلة مهمة من مراحل التاريخ للمجتمع في ليبيا وهو تاريخ تسرب وانتشار الديانة المسيحية في الشمال الأفريقي وليبيا في العصر الوسيط وموقف السلطات الرومانية والبيزنطية منها ، وكذلك المعتقد الذي ساد بهذه المنطقة والذي يرى المؤرخون أنه كان معتقد توحيد (المذهب الأريوسي في العقيد المسيحية ) الذي كان ينكر أن المسيح إنسان وإله في نفس الوقت ، حيث خالف المعتقد الذي فرضته الكنيسة وعلى رأسها الإمبراطور
دور نظم المعلومات الإدارية في تسهيل العمل الاداري (دراسة حالة عن ديوان المحاسبة الليبي فرع مدينة درنة)
Authors: الشريف بالقاسم أ.نجيــــــــــــــــة 
Published: June 2024

نظرا للأهمية الكبرى لموضوع نظم المعلومات الإدارية قد تم تسليط الضوء على هذا الموضوع وربطه بسهولة العمل الاداري ، في إحدى أهم مؤسسات الدولة الليبية (ديوان المحاسبة) – فرع درنة – باعتباره أداة للرقابة على عمل مؤسسات الدولة الحيوية.
ونظرا لصغر حجم عينة الدراسة تم إتباع طريقة المسح الشامل لكافة الأفراد العاملين بديوان المحاسبة الليبي فرع درنة حيث تم تصميم وتوزيع استمارة استبيان ضمت عدة مقاييس لقياس العلاقة بين نظام المعلومات الاداري المتبع داخل ديوان المحاسبة وبين سهولة العمل الاداري ؛ ثم تما تجميع هذه الاستمارات وفرز وتحليل البيانات الواردة بها تحليلا إحصائيا باستخدام برنامج الإحصاء(spss) للوصول الى صحة فرضيات الدراسة من عدمها . وتم التوصل الى عدة نتائج أهمها أهمية نظم المعلومات الإدارية في تسهيل العمل الاداري وثبات صحة الفرضيات داخل المؤسسة .إلا أن هذا النظام يُعاني من بعض العراقيل مثل غياب عنصر التخصص في نظام المعلومات و عدم توفير السرية الكافية في تداول المعلومات بالإضافة الى بطىء شبكة الاتصال المستخدمة لنظام المعلومات. وتم وضع التوصيات اللازمة لزيادة فعالية نظام المعلومات الاداري داخل المؤسسة.
STUDY THE EXISTENCE PERIODIC SOLUTIONS FOR IMPULSIVE NEUTRAL DYNAMIC EQUATIONS WITH INFINITE DELAY ON TIME SCALES
Authors: ELMANSOURI RAFIK , ELMABROK ABDELHAMID , ALSHAIKHLY ALI 
Published: June 2024

Let be a periodic time scale. We use the Krasnoselskii's fixed point theorem to show that the impulsive neutral dynamic equations with infinite delay
have a periodic solution. Under a slightly more stringent conditions we show that the periodic solution is unique using the contraction mapping principle.