bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome Sequence of Deep-Sea Manganese-Oxidizing Bacterium Marinobacter manganoxydans MnI7-9
Authors: Wang Haifang , Li Hang , Shao Zongze , Liao Shuijiao , Johnstone Laurel , Rensing Christopher , Wang Gejiao 
Published: January 2012

ABSTRACT

Here we report the draft genome of
Marinobacter manganoxydans
MnI7-9, isolated from a deep-sea hydrothermal vent in the Indian Ocean and capable of oxidizing manganese even when there is a very high concentration of Mn
2+
. The strain also displayed high resistance and adsorption ability toward many metal(loid)s.
Complete Genome Sequence of Brucella suis VBI22, Isolated from Bovine Milk
Authors: Tae Hongseok , Shallom Shamira , Settlage Robert , Hawkins Gregory N. , Adams L. Garry , Garner Harold R. 
Published: January 2012

ABSTRACT

Brucella suis
is the causative agent of swine brucellosis and is known to be able to infect several different hosts, including cattle, dogs, and horses, without causing disease symptoms. Here we report the complete genome sequence of
Brucella suis
VBI22, which was isolated from raw milk from an infected cow.
Genome Sequence for “Candidatus Mycoplasma haemominutum,” a Low-Pathogenicity Hemoplasma Species
Authors: Barker Emily N. , Darby Alistair C. , Helps Chris R. , Peters Iain R. , Hughes Margaret A. , Radford Alan D. , Novacco Marilisa , Boretti Felicitas S. , Hofmann-Lehmann Regina , Tasker Séverine 
Published: January 2012

ABSTRACT

We present the genome sequence of “
Candidatus
Mycoplasma haemominutum” strain Birmingham 1, a low-pathogenicity feline hemoplasma strain.
Draft Genome Sequence of Probiotic Strain Lactobacillus rhamnosus R0011
Authors: Tompkins Thomas A. , Barreau Guillaume , de Carvalho Vanessa G. 
Published: January 2012

ABSTRACT

Lactobacillus rhamnosus
R0011 is a commercially available probiotic that is widely used in human dietary supplements and pharmaceutical products. We prepared a draft genome sequence consisting of 10 contigs totaling 2,900,620 bases and a G+C content of 46.7% for this strain.
New Strategies in Melanoma: Molecular Testing in Advanced Disease
Authors: Woodman Scott E. , Lazar Alexander J. , Aldape Kenneth D. , Davies Michael A. 
Published: January 2012

Abstract
Melanoma is one of the most aggressive forms of skin cancer. The management of melanoma is evolving rapidly due to an improved understanding of the molecular heterogeneity of this disease and the development of effective, personalized, targeted therapy strategies. Although previous classification systems were based predominantly on clinical and histologic criteria, there is now a strong rationale for adding molecular markers to the diagnostic evaluation of these tumors. Research has shown that the types and prevalence of genetic alterations vary among melanoma subtypes. Thus, rational molecular testing should be based on an understanding of the events that are likely to occur in a given tumor and the clinical implications of test results. This review summarizes the existing data that support the rationale for molecular testing in clinically defined melanoma subtypes. Emerging challenges and controversies regarding the use of various molecular testing platforms, and their implications for clinical testing, are also discussed. Clin Cancer Res; 18(5); 1195–200. ©2012 AACR.
Randomized Study of Autologous Cytokine-Induced Killer Cell Immunotherapy in Metastatic Renal Carcinoma
Authors: Liu Liang , Zhang Weihong , Qi Xiuying , Li Hui , Yu Jinpu , Wei Sheng , Hao Xishan , Ren Xiubao 
Published: January 2012

AbstractPurpose: The therapeutic benefit of the cytokine-induced killer (CIK) cells was unknown in the renal cell carcinoma (RCC). This prospectively randomized study was conducted to evaluate the effects of autologous CIK cell immunotherapy in patients with metastatic clear cell RCCs.Experimental Design: From June 2005 to June 2008, 148 patients with metastatic clear cell RCC were randomized to autologous CIK cell immunotherapy (arm 1, n = 74), or interleukin-2 treatment combination with IFN-α-2a (arm 2, n = 74). The primary endpoint was overall survival (OS) and secondary endpoint was progression-free survival (PFS) evaluated by Kaplan–Meier analyses and treatment HRs with the Cox proportional hazards model.Results: The 3-year PFS and OS in arm 1 were 18% and 61%, as compared with 12% and 23% in arm 2 (P = 0.031 and &lt;0.001, respectively). The median PFS and OS in arm 1 were significantly longer than those in arm 2 (PFS, 12 vs. 8 months, P = 0.024; OS, 46 vs. 19 months, P &lt; 0.001). Multivariate analyses indicated that the cycle count of CIK cell immunotherapy as a continuous variable was significantly associated with prolonged PFS [HR = 0.88; 95% confidence interval (CI), 0.84-0.93; P &lt; 0.001] and OS (HR = 0.58; 95% CI, 0.48–0.69; P &lt; 0.001) in arm 1.Conclusion: The data suggested that CIK cell immunotherapy could improve the prognosis of metastatic clear cell RCC, and increased cycle count of CIK cell treatment could further enhance the beneficial effects. Clin Cancer Res; 18(6); 1751–9. ©2012 AACR.
PET Imaging of Tumor Growth: Not as Easy as It Looks
Authors: Shields Anthony F. 
Published: January 2012

Abstract
Positron emission tomography can be used to image tumor proliferation when combined with appropriate labeled tracers, such as the thymidine analog [18F]-3′-deoxy-3′-fluorothymidine. Although thymidine kinase 1 is the principal mechanism of cell trapping, other variables, such as the cellular level of native thymidine, may need to be considered. Clin Cancer Res; 18(5); 1189–91. ©2012 AACR.
Imexon Induces an Oxidative Endoplasmic Reticulum Stress Response in Pancreatic Cancer Cells
Authors: Sheveleva Elena V. , Landowski Terry H. , Samulitis Betty K. , Bartholomeusz Geoffrey , Powis Garth , Dorr Robert T. 
Published: January 2012

Abstract
Oxidative protein folding in the endoplasmic reticulum (ER) requires strict regulation of redox homeostasis. Disruption of the lumenal redox balance induces an integrated ER stress response that is associated with reduced protein translation, increased chaperone activity, and ultimately cell death. Imexon is a small-molecule chemotherapeutic agent that has been shown to bind glutathione (GSH) and induce oxidative stress in tumor cells; however, the mechanism of cytotoxicity is not well understood. In this report, we investigate the effects of imexon on the integrated ER stress response in pancreatic carcinoma cells. Acute exposure to imexon induces an ER stress response characterized by accumulation of the oxidized form of the oxidoreductase Ero1α, phosphorylation of eIF2α, and inhibition of protein synthesis. An RNA interference chemosensitization screen identified the eukaryotic translation initiation factor eIF2B5 as a target that enhanced imexon-induced growth inhibition of MiaPaCa-2 pancreatic cancer cells, but did not significantly augment the effects of imexon on protein synthesis. Concurrent reduction of intracellular thiols with N-acetyl cysteine reversed imexon activity, however cotreatment with superoxide scavengers had no effect, suggesting thiol binding may be a primary component of the oxidative effects of imexon. Moreover, the data suggest that disruption of the redox balance in the ER is a potential therapeutic target. Mol Cancer Res; 10(3); 392–400. ©2012 AACR.
Obstetric complications and pregnancy-related venous thromboembolism: The effect of low-molecular-weight heparin on their prevention in carriers of factor V Leiden or prothrombin G20210A mutation
Authors: Grandone Elvira , De Stefano Valerio , Tosetto Alberto , Palareti Gualtiero , Margaglione Maurizio , Castaman Giancarlo , Rossi Elena , Ciminello Angela , Valdrè Leila , Legnani Cristina , Luca Tiscia Giovanni , Bafunno Valeria , Carraro Sara , Rodeghiero Francesco , Simioni Paolo , Tormene Daniela 
Published: January 2012

SummaryWhether the administration of low-molecular-weight heparin (LMWH) during pregnancy is effective in preventing obstetric complications and pregnancy-related venous thromboembolism (VTE) in women who are carriers of factor V Leiden (FVL) and/or prothrombin variant G20210A (PTm) is controversial. This observational study investigated the possible efficacy of pharmacological treatment with LMWH ± aspirin (ASA) in pregnancy outcomes in 1,011 pregnancies of 416 women with thrombophilia (FVL and/or PTm). Most patients were chosen on the basis of previous obstetrical complications (36%), or because of familial or personal history of venous/arterial thromboembolism (28% and 18%, respectively); 74 patients (18%) were incidentally identified. The outcome was evaluated according to the type of treatment and of the period of pregnancy when the treatment was started. After adjustment for observation before and after diagnosis of thrombophilia, previous miscarriages and VTE, parity, age and centre, we observed that LMWH had a protective effect on miscarriages (odds ratio [OR] 0.52, 95% confidence interval [CI] 0.29–0.94) and VTE (OR 0.05, 95% CI 0.01–0.21). ASA appeared to have no effect on the prevention of obstetric complications and VTE. A nested analysis performed in 116 women with two or more obstetric complications confirmed that the highest number of live births was recorded in the group under LMWH prophylaxis (OR 0.19, 95% CI 0.05–0.75). These results suggest that LMWH prophylaxis reduces the risk of obstetric complications in carriers of FVL and/or PTm, particularly in those with previous obstetric events. Furthermore, LMWH prophylaxis reduces the risk of pregnancy-related VTE.
Effects of oral anticoagulant therapy and haplotype 1 of the endothelial protein C receptor gene on activated protein C levels
Authors: Medina Pilar , Bonet Elena , Navarro Silvia , Martos Laura , Estellés Amparo , Ferrando Fernando , Vicente Vicente , Bertina Rogier M. , España Francisco 
Published: January 2012

SummaryOral anticoagulants (OACs) reduce activated protein C (APC) plasma levels less than those of protein C (PC) in lupus erythematosus and cardiac patients. Carriers of the H1 haplotype of the endothelial PC receptor gene (PROCR) have higher APC levels than non-carriers. We aimed to confirm these results in a large group of patients treated with OACs because of venous thromboembolism (VTE) and to assess whether the effect is influenced by the PROCR H1 haplotype. We evaluated APC, PC, and factor (F)II levels in 502 VTE patients (158 with and 344 without OACs) and in 322 healthy individuals. Mean APC, PC and FII levels were significantly lower in OAC patients than in patients not taking OACs. During anticoagulant therapy, the FII/PC ratios were independent of the PC values, whereas APC/FII and APC/PC ratios significantly increased when FII and PC levels decreased. Of the 22 OAC patients carrying the H1H1genotype, 11 (50%) showed APC/PCag ≥2.0 and 10 (45%) APC/ FIIag ratios ≥2.0, whereas for the 49 OAC patients non-carrying the H1 haplotype these figures were 6 (12%) and 4 (8%), respectively (p<0.001). Barium citrate adsorption of plasma from OAC patients showed that most of the circulating free and complexed APC, but only part of PCag, is fully carboxylated. In conclusion, during anticoagulant therapy VT patients have APC levels disproportionately higher than the corresponding PC levels, mainly due to the presence of the PROCR H1 haplotype. Furthermore, a sufficiently carboxylated PC Gla-domain seems to be essential for PC activation in vivo.
Vasodilator-stimulated phosphoprotein-phosphorylation assay in patients on clopidogrel: Does standardisation matter?
Authors: Bruno Veronika , Willheim Martin , Hübl Wolfgang , Wojta Johann , Huber Kurt , Freynhofer Matthias K. 
Published: January 2012

SummaryThe vasodilator-stimulated phosphoprotein-phosphorylation (VASP-P) flow-cytometric assay is mainly used in clinical trials to measure thienopyridine effects. However, there are remarkable differences in the reported optimal cut-offs, ranging from 48–61% platelet reactivity index (PRI). We therefore investigated whether a lack of standardisation might explain the differences in the cut-offs. We measured VASP-P in 62 individuals. PRI was calculated using the mean, geometric mean and median fluorescence intensities (FI). Stability of the blood-samples (time-to-assay, 0–2 days) and stability of the processed samples (0–120 minutes) within the recommended time-span were tested. Time-to-assay significantly influenced the PRI (p<0.001): the PRI from mean FI after two days was lower compared to values on day 1 (52 ± 22.9 vs. 57.7 ± 24.1, p<0.001). The PRI from the geometric mean FI after two days was lower compared to day 0 as well as day 1 (51.3 ± 23 vs. 58.2 ± 24.2 and vs. 59.1 ± 23.7, both p<0.001). The PRI from median FI was stable over time (day 0: 59.1 ± 25%, day 1: 59.7 ± 24.1% and day 2: 56.4 ± 23.9%, all p=ns). Furthermore, the lag time of the processed samples significantly altered the PRI (all p<0.001) with a maximum difference for PRI based on geometric mean FI after 90 minutes compared to baseline (Δ=3.92%PRI, p<0.001). The differences in the reported cut-offs might be explained by a lack of standardisation. More precise standardisation is inevitable, as the PRI significantly depends on the method of calculation, the time-to-assay as well as on the lag time after processing. Tolerably stable results were obtained for the PRI from the median FI.
MicroRNAs regulating lipid metabolism in atherogenesis
Authors: Rayner Katey J. , Fernández-Hernando Carlos , Moore Kathryn J. 
Published: January 2012

SummaryMicroRNAs have emerged as important post-transcriptional regulators of lipid metabolism, and represent a new class of targets for therapeutic intervention. Recently, microRNA-33a and b (miR-33a/b) were discovered as key regulators of metabolic programs including cholesterol and fatty acid homeostasis. These intronic microRNAs are embedded in the sterol response element binding protein genes, SREBF2 and SREBF1, which code for transcription factors that coordinate cholesterol and fatty acid synthesis. By repressing a variety of genes involved in cholesterol export and fatty acid oxidation, including ABCA1, CROT, CPT1, HADHB and PRKAA1, miR-33a/b act in concert with their host genes to boost cellular sterol levels. Recent work in animal models has shown that inhibition of these small non-coding RNAs has potent effects on lipoprotein metabolism, including increasing plasma high-density lipo-protein (HDL) and reducing very low density lipoprotein (VLDL) triglyce-rides. Furthermore, other microRNAs are being discovered that also target the ABCA1 pathway, including miR-758, suggesting that miRNAs may work cooperatively to regulate this pathway. These exciting findings support the development of microRNA antagonists as potential therapeutics for the treatment of dyslipidaemia, atherosclerosis and related metabolic diseases.
Nitroglycerin prevents coagulopathies and foetal death associated with abnormal maternal inflammation in rats
Authors: Cotechini Tiziana , Othman Maha , Graham Charles H. 
Published: January 2012

SummaryInflammation-associated foetal loss is often linked to maternal coagulopathies. Here, we characterised the role of maternal inflammation in the development of various systemic maternal coagulopathies and foetal death during mid-to-late gestation in rats. Since nitric oxide (NO) functions as an inhibitor of platelet aggregation and antioxidant, we also tested whether the NO mimetic nitroglycerin (glyceryl trinitrate, GTN) prevents inflammation-associated coagulopathies and foetal death. To induce chronic inflammation, pregnant Wistar rats were injected with low-doses of lipopolysaccharide (LPS; 10–40 μg/kg) on gestational days (GD) 13.5–16.5. To determine whether the effects of inflammation are mediated by tumour necrosis factor-α (TNF-α), the TNF-α inhibitor etanercept was injected on GD 13.5 and 15.5. Controls consisted of rats injected with saline. GTN was administered to LPS-treated rats via daily application of a transdermal patch on GD 12.5–16.5. Using thromboelastography (TEG), various coagulation parameters were assessed on GD 17.5; foetal viability was determined morphologically. Reference coagulation parameters were established based on TEG results obtained from control animals. LPS-treated rats exhibited distinct systemic coagulopathies: hypercoagulability, hypocoagulability, hyperfibrinolysis, and disseminated intravascular coagulation (DIC) stages I and III. A specific foetal death coagulation phenotype was observed, implicating TEG as a potential tool to identify inflammation-induced haemostatic alterations associated with pregnancy loss. Treatment with etanercept reduced the incidence of coagulopathy by 47%, while continuous delivery of GTN prevented foetal death and the inflammation-induced coagulopathies. These findings provide a rationale for investigating the use of GTN in the prevention of maternal coagulopathies and inflammation-mediated foetal death.
Cardiomyogenesis in the Developing Heart Is Regulated by C-Kit–Positive Cardiac Stem Cells
Authors: Ferreira-Martins João , Ogórek Barbara , Cappetta Donato , Matsuda Alex , Signore Sergio , D'Amario Domenico , Kostyla James , Steadman Elisabeth , Ide-Iwata Noriko , Sanada Fumihiro , Iaffaldano Grazia , Ottolenghi Sergio , Hosoda Toru , Leri Annarosa , Kajstura Jan , Anversa Piero , Rota Marcello 
Published: January 2012

RETRACTION
This article has been retracted:

Circ Res
. 2018;123:e00. DOI: 10.1161/RES.0000000000000252.
9-Cis Retinoic Acid Promotes Lymphangiogenesis and Enhances Lymphatic Vessel Regeneration
Authors: Choi Inho , Lee Sunju , Kyoung Chung Hee , Suk Lee Yong , Eui Kim Kyu , Choi Dongwon , Park Eun Kyung , Yang Dongyun , Ecoiffier Tatiana , Monahan John , Chen Wen , Aguilar Berenice , Lee Ha Neul , Yoo Jaehyuk , Koh Chester J. , Chen Lu , Wong Alex K. , Hong Young-Kwon 
Published: January 2012

Background—
The lymphatic system plays a key role in tissue fluid homeostasis and lymphatic dysfunction caused by genetic defects, or lymphatic vessel obstruction can cause lymphedema, disfiguring tissue swelling often associated with fibrosis and recurrent infections with no available cures to date. In this study, retinoic acids (RAs) were determined to be a potent therapeutic agent that is immediately applicable to reduce secondary lymphedema.

Methods and Results—

We report that RAs promote proliferation, migration, and tube formation of cultured lymphatic endothelial cells by activating fibroblast growth factor receptor signaling. Moreover, RAs control the expression of cell-cycle checkpoint regulators such as p27
Kip1
, p57
Kip2
, and the aurora kinases through both an Akt-mediated nongenomic action and a transcription-dependent genomic action that is mediated by Prox1, a master regulator of lymphatic development. Moreover, 9-cisRA was found to activate in vivo lymphangiogenesis in animals in mouse trachea, Matrigel plug, and cornea pocket assays. Finally, we demonstrate that 9-cisRA can provide a strong therapeutic efficacy in ameliorating experimental mouse tail lymphedema by enhancing lymphatic vessel regeneration.

Conclusion—
These in vitro and animal studies demonstrate that 9-cisRA potently activates lymphangiogenesis and promotes lymphatic regeneration in an experimental lymphedema model, presenting it as a promising novel therapeutic agent to treat human lymphedema patients.
Like Father, Like Son: Poetic Strategies in "The Middle Brother" (Madhyama-vyāyoga) Attributed to Bhāsa
Authors: Salomon Richard 
Published: January 2012

AbstractThe one-act Sanskrit drama Madhyama-vyāyoga or "The Middle Brother" attributed to Bhāsa describes an oedipal encounter between the Pāndava hero Bhīmasena and his half-demon son Ghatotkaca. The author utilizes subtle techniques of word choice and strategic repetition of key words, particularly sadrśa 'like, similar,' to hint at the underlying similarity of the superficially unlike pair. This keyword technique, which is found only sporadically in Sanskrit, is compared to similar techniques in other literatures, particularly the Leitwortstil characteristic of Biblical Hebrew.
Ksatriyas in the Kali Age? Gāgābhatta & His Opponents
Authors: Deshpande Madhav 
Published: January 2012

AbstractThis paper deals with the history of the disputes regarding the Ksatriya status of the local ruler Shivaji and the Cāndrasenīya Kāyastha Prabhu (CKP) community of Maharashtra. The origin of these disputes lies in the wider dispute concerning whether there are any true Ksatriyas in the Kali age. The CKPs of Maharashtra claimed to be Ksatriyas and thus entitled for the rite of Upanayana, while the dominant regional Brahmin opinion was that they were Śūdras and not entitled for the Upanayana. The dispute broke out a few years before Shivaji's coronation, and to the discomfort of the local Brahmins, Gāgābhatta of Banaras settled it in favor of the Kāyasthas in his work, the Kāyasthadharmadīpa. In decades after Shivaji's death, the dispute broke out again, and within Maharashtra, gradually the Dharmaśāstric opinion shifted against the views of Gāgābhatta, and toward the end of the rule of the last Peshwa, this dispute was raised again by Nīlakantha Śāstri Thatte in Pune against the Kāyasthas. I have traced the lineage of Nīlakantha Thatte, through his teacher Vaidyanātha Pāyagunde, to his teacher, the great Nāgeśabhatta of Banaras. Nāgeśsabhatta produced his Vrātyatāprāyaścittanirnaya at a śāastrasabhā in Jaipur, where he argued that there were no pure Ksatriyas surviving in the Kali age, and that the impure ones do not have the eligibility for Upanayana through some expiation. So the Kāyasthas could not claim to be genuine Ksatriyas either. It was this opinion of Nāgesabhatta, counter to the opinion of Gāgābhatta, that steadily gained popularity among the Pune Brahmins during the rule of the Peshwas, finally reflected in the activities of Nīlakantha Thatte.
Julius Evola and the UR Group
Authors: Hakl Hans Thmas 
Published: January 2012

AbstractDie Gruppe von UR, die von 1927–1929 bestand, wurde im Wesentlichen vom italienischen Mathematiker, Neu-Pythagoreer und Hochgradfreimaurer Arturo Reghini sowie von Julius Evola begründet, der damals bereits seine dadaistische und philosophische Periode beendet und sich intensiv mit fernöstlicher Spiritualität beschäftigt hatte. Auf Grund ihres umfangreichen Lehrmaterials und wegen der hoch qualifizierten Mitglieder (darunter Altphilologen, der Begründer der Freudschen Psychoanalyse in Italien, Literaten, erfahrene Mitglieder anderer magischer Gruppierungen sowie die Spitze der Anthroposophie in Italien) muss die Gruppe von UR zu den interessantesten magischen Bünden des 20. Jahrhunderts gerechnet werden. UR veröffentlichte zur Zeit ihres Bestehens regelmäßig Monatshefte, die insgesamt auf über eintausendzweihundert Seiten konzentriertes praktisches Material aus allen Epochen und Weltgegenden vorlegte. Das Ziel der Gruppe war nicht Mystik oder schöngeistige Philosophie, sondern einen tatsächlicher Zugang zu transzendenten Bereichen aufzuzeigen. Dazu wurde ein nachvollziehbarer Stufenweg vorgezeichnet und in möglichst klaren Worten erläutert. Praktische Texte aus der Alchimie, dem Tantrismus, Buddhismus, der antiken Theurgie und der Sexualmagie wurden zusätzlich beigezogen. Der Stufenweg sollte zu einer echten Gottwerdung (nicht Gottähnlichkeit) führen nach dem Satz Meister Eckharts: "Soll ich Gott unmittelbar erkennen, so muss ich schlechthin Er und Er muss ich werden." Die Möglichkeit dazu bot der Aufbau eines unzerstörbaren "Diamantkörpers", der auch den physischen Tod überstehen sollte. Die Gruppe von UR war in zwei Fraktionen aufgeteilt. Die eine, die praktisch verwertbare Texte übersetzte und kommentierte und eine zweite innere, die diesen Weg praktisch verfolgte und die dabei gemachten Einzel- und Gruppenerfahrungen in unmissverständlicher Sprache beschrieb.