bluuebunny/crossref_metadata_2025_split huggingface dataset:
Magnitocaloric Effect in RCO<sub>5</sub> (R = Gd, Tb, Dy, Ho) Alloys
Authors: Utarbekova M. V. , Orshulevich M. A. , Bataev D. S. , Fazlitdinova A. G. , Taskaev S. V. 
Published: April 2024

Abstract—Experimental studies of magnetic and magnetocaloric properties of polycrystalline RCo5 alloys (R = Gd, Tb, Dy, Ho) in external magnetic fields up to 3 Tesla have been carried out. The results of magnetic measurements have shown that these compounds have small coercivity and reach saturation in small fields. It was found that the magnetocaloric effect in the studied co-compounds is observed in a wide temperature range, and for the intermetallides TbCo5, DyCo5, HoCo5 has several regions of existence, comparable in magnitude of the effect. The presence of several MCE existence intervals is caused by a series of magnetic phase transitions in these ferrimagnetic compounds.
Extracellular Zn Detoxication by <i>Penicillium chrysogenum</i> and <i>Aspergillus niger</i>
Authors: Sazanova K. V. , Zelenskaya M. S. , Korneev A. V. , Vlasov D. Yu. 
Published: April 2024

Microfungi are promising objects for bioremediation due to their ability to convert metals into forms that are less mobile and accessible to organisms. The study showed that the fungi Penicillium chrysogenum and Aspergillus niger have physiological mechanisms of extracellular Zn detoxification at its initial concentration of 250 µmol – 2 mmol in the medium. At concentrations of 250–500 µmol Zn promotes the accumulation of biomass and abundant sporulation of A. niger and Penicillium chrysogenum, and at concentrations of 1–2 mmol inhibits the growth of fungi. Extracellular Zn detoxification in Aspergillus niger culture occurs by the formation of dihydrate zinc oxalate katsarosite, due to the active biosynthesis of oxalic acid in A. niger. The main mechanism of zinc detoxification in Penicillium chrysogenum culture was the formation of zinc phosphate hopeite. The formation of zinc phosphate hopeite under the action of the fungus was established for the first time. The key factors that determine the direction of the processes of formation of extracellular mineral phases are the amounts of EPS and oxalic acid produced by fungi, as well as changes in the pH of the medium during the growth of cultures.
Cytokine Profile of Myocardial Cells in Coronary Heart Disease and Ischemic Cardiomyopathy
Authors: Stelmashenko A. I. , Andreev S. L. , Litvinova L. S. , Malashchenko V. V. , Todosenko N. M. , Gazatova N. D. , Khlusov I. А. , Shipulin V. M. 
Published: April 2024

In the present work, we carried out a comparative analysis of myocardial cytokine profile in patients with coronary heart disease (CHD) and in patients with ischemic cardiomyopathy (ICMP) associated with CHD. The concentrations of 41 cytokines secreted by 24-hour myocardial tissue culture intraoperatively sampled from the right atrial auricle (RAA, control) and peri-infarct left ventricular zone (PZ-LV) were determined by flow fluorimetry using a multiplex test system. The aim was to study in vitro cytokine profile of myocardial cells to search for possible predictors of adverse outcomes of surgical treatment of patients with CHD and ICMP. Myocardial secretion of proinflammatory molecules GM-CSF and IFN-γ increased significantly (up to 78-80 pg/g, p0.05) in patients with ICMP associated with CHD in contrast to zero values in CHD. At the same time, there was a three-fold decrease in the concentration of fractalkin 3 ligand (Flt-3L; FMS-like tyrosine kinase 3 ligand). A decrease in Flt-3L secretion was observed in the PZ-LV in comparison with the RAA. In addition, compared with RAA, concentrations of fibroblast growth factor-2 (FGF-2), platelet-derived growth factor-AB/BB (PDGFAB/BB), interleukins IL-15 and IL-4, and a regulated upon activation, normal T cell expressed and secreted (RANTES; CCL5) were strongly reduced in PZ-LV myocardial tissue culture. Differences in the course of CHD and ICMP are discussed, and possible predictors of surgical treatment risk in patients of the two groups are suggested using correlation and regression analyses. Proinflammatory cytokines (IL-5, IL-6) and chemokines (Flt-3L, IL-8), as well as angiogenesis factors (VEGF) and angiostasis (IP-10), are proposed to be considered as potential markers of adverse outcome of surgical treatment of cardiovascular disease.
Study of the Protein Composition of Dry Blood Spots of Healthy Volunteers in the Experiment with Hypomagnetic Conditions
Authors: Kashirina D. N. , Larina I. M. , Nikolaev E. N. , Tyuzhin M. G. , Popova O. V. , Kukanov V. Yu. , Rusanov V. B. , Kononikhin A. S. , Brzhozovskiy A. G. , Pastushkova L. Kh. , Orlov O. I. 
Published: April 2024

Hypomagnetic conditions will be an integral part of the complex of factors affecting cosmonauts in long-term interplanetary missions outside the Earth’s magnetic field. Adaptation of the human body under these conditions will affect the regulatory processes in various physiological systems, the molecular pathways of which, with the participation of proteins, have not yet been studied. In order to fill this gap, dry blood spots collected on a special paper from healthy volunteers were studied by proteomic methods in two sessions of a model experiment: with hypomagnetic conditions and in a “placebo” session. The experiment was carried out by double-blind control, with the participation of the same volunteers in both sessions. 1219 different proteins were semiquantitatively determined in the samples. In the hypomagnetic conditions series, changes in three proteins: tropomyosin alpha-3 chain (TPM3), abhydrolase domain-containing protein 14B (ABHD14B), and acetylcholinesterase-associated protein (CUTA), relative to individual values before the experiment series were detected. However, comparison of the data obtained in the “placebo” session smoothed out the effect of hypomagnetic conditions on the changes in the aforementioned proteins. The results obtained, in our opinion, may either mean the absence of the influence of hypomagnetic conditions with a short exposure and the absence of a cumulative effect, or they are unreliable due to the insufficient number of subjects in the sample.
Dynamics of TP, HF-, LF- and VLF- Waves of the Cardiointervalogram (in Clinostasis Conditions) of an Elite Ski Racer in the Preparatory, Competitive and Transitional Periods, Depending on the Volume and Intensity of Training Loads
Authors: Kataev D. A. , Tsirkin V. I. , Zavalin N. S. , Morozova M. A. , Trukhin A. N. , Trukhina S. I. 
Published: April 2024

In order to study the mechanisms of adaptation to loads requiring high endurance, the 27-year-old master of Sports of Russia in cross-country skiing repeatedly recorded a cardiointervalogram (CIG) under clinostasis conditions, estimating TP, absolute power (mc2) of LF-, HF- and VLF-waves and the relative (as a percentage of TP) power of these waves, i.e. LF%, HF% and VLF%. They were compared with the volume (Vkm, Vmin) and intensity (Nhr) of training loads. The volume of loads was maximum in the preparatory period (21 km/day) and it is minimal in the transition period (18 km/day), and their intensity throughout the annual cycle was stable (working pulse – 120–121 beats/min). With the change in the volume of loads, the values of the KIG indicators also changed. So, in the preparatory period, the medians of TP, the power of HF-, LF- and VLF-waves, as well as VLF% increase; in this period, with an increase in the volume of loads (Vkm), the values of VLF% increase. In the competitive period, the medians of TP, the power of HF-, LF- and VLF-waves and VLF% remain at a high level. In the transition period, the median of TP, the power HF-, LF- and VLF-waves, as well as LF% and VLF%, but the median of HF% increases. For the annual cycle, a direct dependence of the median of TP on the volume of loads (Vkm) and the median power of VLF waves on the volume (Vkm) and intensity (Nhr) of the load was revealed. It is postulated that the values of TP, HF-, LF-, and VLF-waves, as well as VLF% (in clinostasis) reflect the influence of the Cholinergic system on the heart, while VLF% probably reflects the intensity of synthesis of non-neuronal heart’s acetylcholine, and the values of LF% and HF% reflect the formation of anxiety in connection with upcoming starts.
Relation of Neural Beta-Oscillations of the Subthalamic Nucleus to Clinical Symptoms of Parkinson’s Disease
Authors: Sayfulina K. E. , Filyushkina V. I. , Gamaleya A. A. , Tomskiy A. A. , Sedov A. S. , Belova E. M. 
Published: April 2024

Beta oscillations within the subthalamic nucleus (STN) are proposed to serve as biomarker for Parkinson’s disease. A wealth of data indicate essential functional differences between two subbands – low beta (13–19 Hz) and high beta (20–30 Hz), but there is still no full understanding of their specialization. We collected postoperative LFP data using externalized leads from 6 PD patients with implanted bilateral STN DBS electrodes before and after levodopa administration. We studied the relationship between parameters of low and high beta activity and motor symptom scales (UPDRS3, bradykinesia and rigidity). Mean PSD in both subbands decreased after levodopa administration. Low beta mean PSD was positively correlated with all three inspected motor scales (UPDRS3, rigidity and bradykinesia), while high beta mean PSD correlated only with rigidity. Most of the oscillatory peaks were concentrated within the high beta band. Their number decreased after levodopa administration, still leaving a prominent part unaffected. Almost all the low beta peaks compiling small initial number have disappeared after levodopa administration, thus we analyzed only high beta peak parameters. High beta peak frequency and amplitude changed with OFF-ON transition and were correlated with only rigidity scores. Our findings indicate that, although both low and high beta respond to medication state, there are functional differences between the subbands: low beta reflects motor symptoms non-specifically, while high beta is more specialized and reflects rigidity.
Spectral Characteristics of Cardinal Vowels as Indicators of the Auditory Speech Feedback Control in Patients with Moderate and Moderately Severe Chronic Postlingual Sensorineural Hearing Loss
Authors: Shtin K. S. , Lunichkin A. M. , Gvozdeva A. P. , Golovanova L. E. , Andreeva I. G. 
Published: April 2024

Chronic sensorineural hearing loss (SNHL) is characterized by an increase in hearing thresholds at basic speech frequencies, which implies the auditory speech feedback control worsening and, as a result, changes of speech characteristics. A hypothesis was tested that such worsening can manifest itself in an increase of F0, F1, F2 formants of speech vowel sounds in patients with moderate and moderately severe postlingual SNHL. Recordings of elicited speech were performed for young and middle age women (36–59 years): 7 women speakers with moderate SNHL who did not use hearing aids; 5 women speakers with moderately severe SNHL who were hearing aid users but were not using them during the recordings; a control group of 12 normally hearing women speakers. An assessment of F0, F1 and F2 of stressed vowels [a], [i], [u] and calculations of vowels’ centralization indices – vowel space area, vowel formant centralization ratio and the second formant ratio (F2i/F2u), were performed. All the studied spectral indices in groups of patients with postlingual SNHL were similar to those in the control group, no statistically reliable differences were revealed.
Vasoactive and Neuroprotective Action of с-Jun-N-Terminal Kinases Inhibitor in Rats with Chronic Brain Hypoperfusion
Authors: Zhilyaev S. Yu. , Platonova T. F. , Khlebnikov A. I. , Schepetkin I. A. , Demchenko I. T. , Atochin D. N. 
Published: April 2024

The aim of this study was to evaluate the vasoactive and neuroprotective effects of c-Jun-N-terminal kinase inhibitor IQ-1 (11H-indeno[1,2-b]quinoxalin-11-one oxime) in chronic cerebral hypoperfusion caused by irreversible bilateral ligation of carotid arteries. Cerebral blood flow was measured quantitatively (hydrogen clearance method) simultaneously in the parietal cortex, hippocampus, substantia nigra, and striatum of the brain of awake rats. It was found that ligation of the carotid arteries caused a decrease in blood flow in the brain structures with a more pronounced decrease in the cortex (by 48% of the initial level) and with the smallest drop in the substantia nigra (by 25% of the initial level). The reduced level of blood flow persisted for 14 days of measurements. The responses of the cerebral vessels to hypercapnic probes (5% CO2) were lost during the 2-week hypoperfusion period, and the neurological status of the animals did not improve. The administration of IQ-1 (50 mg/kg, intraperitoneally, every 48 hours for 14 days) was accompanied by an increase in blood flow in all brain structures. The maximum increase in blood flow was observed in the striatum and the minimum in the substantia nigra. After the administration of IQ-1, the sensitivity of the cerebral vessels to the hypercapnic stimulus was restored and the neurological state of the animals significantly improved by the end of the second week of cerebral hypoperfusion. The results show that the use of the JNK inhibitor can reduce cerebrovascular disorders and associated neurological disorders in hypoperfusion brain injury.
Influence of Immune System Activation in Early Ontogenesis on Aggressiveness and Sexual Motivationi Adult Wistar Rats
Authors: Pavlova I. V. , Broshevitskaya N. D. 
Published: April 2024

Stresses in early ontogenesis can influence anxiety-depressive, defensive and social behavior, as well as the immune and hormonal reactivity of adult animals. The model of early proinflammatory stress assumes activation of the immune system by the introduction of bacterial lipopolysaccharide (LPS) in early ontogenesis. Whether neonatal LPS stress impacts aggressive behavior or sexual motivation is still unknown. Pathological aggressiveness and disorders in sexual behavior are characteristic of many neuropsychiatric diseases, therefore, the study of the prerequisites for their occurrence is highly relevant. On the 3rd and 5th day after birth, the rats were injected with either LPS at a dose of 50 mcg/kg (LPS group) or saline solution (SAL group, control). The aggressive behavior of adult rats in the resident-intruder test after a week of social isolation was analyzed, as well as the sexual preference of males or females in a three-chamber test. The behavior of rats was compared with baseline levels of corticosterone and interleukin-1beta in blood serum. The males of the LPS group compared with the control had more attacks on the intruder in the resident-intruder test, and the number of attacks did not decrease by the end of the experiment. The males of the LPS group had an increased interaction time with the female at the diestrus stage in the social preference test of males and females. There were no changes in aggressive behavior in females after the administration of LPS in early ontogenesis. The baseline level of interleukin-1beta was higher in males, and corticosterone was higher in females, there were no differences between the LPS and PHYS groups in biochemical parameters. The results obtained indicate an increase in aggressiveness provoked by social isolation, as well as sexual motivation in adult males after early pro-inflammatory stress, i.e., a change in the motivational and emotional component in social behavior.
Influence of Forced Running Loads on the Content of Na<sup>+</sup>/K<sup>+</sup>-ATPase Isoforms and Monovalent Cations in Skeletal Muscles of Mice with a Model of Type II Diabetes Mellitus
Authors: Zakharova A. N. , Milovanova K. G. , Orlova A. A. , Dyakova E. Yu. , Kalinnikova Yu. G. , Kollantay O. V. , Shuvalov I. Yu. , Chibalin A. V. , Kapilevich L. V. 
Published: April 2024

The effect of forced running for 1 hour daily for 4 weeks on the content of Na+/K+-ATPase isoforms and monovalent cations in the skeletal muscles of mice with a model of type II diabetes mellitus (DM-II) was studied. To form a model of the disease, a high-fat diet was used, and physical activity in the form of forced running was carried out for 4 weeks. The content of Na+/K+-ATPase isoforms and Na+ and K+ ions in muscle tissue of m. gastrocnemius was determined by Western blotting and atomic absorption spectrophotomery, respectively. It has been shown that the formation of DM-II in mice is accompanied by changes in the content of Na+/K+-ATPase alpha 1 and 2 isoforms in muscle tissue. The effect of forced running loads on the content of Na+/K+-ATPase in muscle tissue is significant and primarily differs in age groups. One can also note a certain dependence of the influence of forced running loads on the content of this enzyme on the time of their use. In young animals, changes in the concentrations of monovalent sodium and potassium cations after forced running loads were less pronounced. In aged mice, against the background of forced loads, an increase in the content of sodium and decrease in the content of potassium in muscle tissue was observed. The detected changes in monovalent cations content in the muscle tissue of mice with diabetes mellitus II under the influence of forced running loads may play a role in the implementation of the metabolic effects of physical activity.
Trifluoroethylacrylate-Substituted Polymethylsiloxane—Promising Membrane Material for Separating an Abe Fermentation Mixture
Authors: Grushevenko Е. А. , Rokhmanka Т. N. , Balynin A. V. , Golubev G. S. , Borisov I. L. 
Published: April 2024

This work is aimed at obtaining a membrane material that is resistant to the formation of a precipitate on the surface upon contact with an ABE fermentation mixture and has a good separating ability during the pervaporation isolation of n-butanol from a water–alcohol mixture. In this regard, in this work, for the first time, the creation of pervaporation membranes based on polymethyltrifluoroethylacrylatesiloxane (F3-Acr), as well as a copolymer of polydecylmethylsiloxane and polymethyltrifluoroethylacrylatesiloxane (C10-F3-Acr) is proposed. In comparison with polydecylmethylsiloxane (C10), the structure and sorption properties of the developed membrane materials for n-butanol, ethanol and acetone were studied. It should be noted that the highest sorption of n-butanol is characteristic of C10-F3-Acr (0.46 g/g). Changes in surface properties were assessed by the contact angle and elemental composition of the surface before and after exposure for 1 month in a fermentation medium. The transport and separating properties of the synthesized membrane materials were studied in the vacuum pervaporation mode during the separation of a model ABE fermentation mixture. It was shown that the introduction of a fluorine-containing substituent into the side chain of polysiloxane made it possible to increase the hydrophilicity of the polymer: the water flow for F3-Acr was 0.7 × 10–6 kg m m–2 h–1, which is almost 3 times higher than for C10. It is worth noting the positive effect of the combination of C10 and F3-Acr groups in polysiloxane. Thus, with an increase in the total flow by 60% compared to the C10 membrane, the values of the separation factor for n-butanol, acetone, and ethanol were 40.5, 32.7, and 4.3 and increased by 6, 15, and 12%, respectively, compared to the C10 membrane. For the C10-F3-Acr membrane, the pervaporation separation index for n-butanol, acetone, and ethanol was 136, 109, and 11. Therefore, this membrane is twice as efficient as C10. Taking into account the absence of detectable contamination of the surface of the membrane material with fermentation products, one can note the high potential of the C10-F3-Acr membrane for the task of isolating alcohols from the ABE fermentation mixture.
ANALISIS POLA ASUH ORANG TUA TERHADAP PERKEMBANGAN KOGNITIF ANAK USIA DINI
Authors: Aulia Keysha 
Published: April 2024

Perkembangan kognitif anak usia dini di TK Islam Prajamuda merupakan aspek penting dalam pembentukan potensi kognitif dan kemampuan belajar mereka. Dalam konteks ini, pola asuh orang tua memainkan peran krusial dalam mempengaruhi perkembangan kognitif anak. Studi ini bertujuan untuk menganalisis pola asuh orang tua dan dampaknya terhadap perkembangan kognitif anak usia dini. Melalui penelitian dan analisis yang dilakukan lewat deskriptif kualitatif, ditemukan bahwa pola asuh orang tua yang mendukung memberikan pengaruh positif terhadap perkembangan kognitif anak di TK Islam Prajamuda. Kesimpulan dalam penelitian ini ialah orang tua yang memberikan stimulus yang tepat dan bervariasi, komunikasi yang baik, serta tantangan yang sesuai dengan tingkat perkembangan anak, cenderung mempercepat perkembangan kognitif anak. Diharapkan penelitian dapat memberikan pengaruh pada anak usia dini di TK Islam Prajamuda terkhusus pada perkembangan kognitif yang dimiliki.
Kata Kunci : Pola Asuh Orang Tua, Perkembangan Kognitif.
Early childhood cognitive development in Prajamuda Islamic Kindergarten is an important aspect in the formation of their cognitive potential and learning abilities. In this context, parenting style plays a crucial role in influencing children's cognitive development. This study aims to analyze parenting styles and their impact on early childhood cognitive development. Through research and analysis conducted through descriptive qualitative, it was found that supportive parenting styles had a positive influence on children's cognitive development in Prajamuda Islamic Kindergarten. The conclusion in this study is that parents who provide appropriate and varied stimuli, good communication, and challenges that are appropriate to the child's developmental level tend to accelerate children's cognitive development. It is hoped that the research can have an impact on early childhood in Prajamuda Islamic Kindergarten, especially on their cognitive development.
Keywords : Parenting Patterns, Cognitive Development
ANALISIS KEGIATAN GERAK DAN LAGU TERHADAP PEMAHAMAN KONSEP ALJABAR (POLA) PADA ANAK USIA 5-6 TAHUN DI TAMAN KANAK-KANAK
Authors: Sri Wilanda Novia , Jihan Jihan Faninda Ridanti , Yaswinda Yaswinda 
Published: April 2024

Dalam perkembangan pendidikan anak usia dini, penting bagi anak untuk mengembangkan konsep matematika sejak dini. Salah satu konsep yang penting untuk diperkenalkan adalah konsep aljabar (pola). Pendidikan anak usia dini merupakan tahap awal dalam perkembangan anak yang sangat penting. Pada usia 5-6 tahun, anak-anak masih dalam periode golden age, di mana mereka memiliki kemampuan belajar yang luar biasa. Masa ini merupakan waktu yang tepat untuk memperkenalkan konsep-konsep matematika dasar seperti aljabar (pola). Aljabar adalah salah satu cabang matematika yang penting dalam memahami pola dan hubungan matematika. Dalam upaya meningkatkan pemahaman konsep aljabar pada anak usia 5-6 tahun di taman kanak-kanak, kegiatan gerak dan lagu dapat menjadi salah satu cara yang efektif. Kegiatan gerak dan lagu dapat membantu anak-anak untuk memahami konsep aljabar melalui pengalaman nyata dan interaktif. Gerak dan lagu juga merupakan salah satu yang dapat melihatkan ekspresi anak baik itu senang, sedih, marah, dan takut dipadukan dengan lagu atau nyanyian sehingga anak bisa meluapkan ekspresinya dan membebaskan dirinya dari ketegangannya melalui gerakan yang diiringi oleh lagu. Penelitian ini bertujuan untuk mengetahui bagaimana kegiatan pelaksanaan gerak dan lagu terhadap pemahaman konsep aljabar (pola) pada anak usia 5-6 tahun di TK IT Abatatsa Lahat dan TK restu Ilahi Pesisir Selatan.
Kata Kunci : Gerak, lagu, aljabar, pola, anak usia dini