bluuebunny/crossref_metadata_2025_split huggingface dataset:
Digital analysis of the changes in carcinomatous conjunctiva using Aperio nuclear v9 algorithm
Authors: ASOKLIS R , PAULAVICIENE R , PETROSKA D 
Published: August 2012

AbstractPurpose To analyze and evaluate biological and morphometrical changes and differences of healthy and carcinomatous conjunctiva using Aperio nuclear v9 algorithm.Methods A pilot study for the objective evaluation of carcinomatous conjunctiva in 6 cases. There were analyzed 6 excised conjunctival tumors specimens from 6 patients (6 eyes). Medium age of the patients was 68 years. There were two males and four females. In 3 patients (3 eyes) conjunctival tumor was excised, cryoaplication and amniotic membrane transplantation was performed. In 3 patients excisional biopsy was performed without additional surgical manipulations. Conjunctival specimens were stained with hematoxilin and eosin. Immunohistochemical (IHC) staining with a proliferation marker of cells Ki‐67 was performed for all specimens. Digital slides of the IHC of Ki‐67 from these biopsies were obtained by Aperio ScanScope GL. Aperio Nuclear V9 algorithm was applied to assay proliferation indices (PI) of nuclei stained with Ki‐67 and size of all nuclei in both abnormal and normal conjunctiva.Results There were analyzed from 1585 till 5020 malignant and 242‐714 normal cells. PI of Ki‐67 ≥19% (range 19.3‐62.1%) was found in carcinomatous conjunctiva comparing with normal conjunctiva PI ≤13.5% (range 1.9‐21.6%) in the same patients. No significant difference was found between nucleus size in pathological (mean 41 µm2, range 33‐64 µm2) and normal conjunctiva (mean 35.5 µm2, range 34‐36 µm2).Conclusion Aperio nuclear algorithm can be used for carcinomatous and healthy conjunctiva analysis. Further studies of the malignant and healthy eye tissues using larger cohort are required.
Sebaceous carcinoma : diagnosis and therapeutic difficulties
Authors: GAVARD‐PERRET A , LAGIER J , CAUJOLLE JP 
Published: August 2012

AbstractPurpose Sebaceous carcinoma is a malignant neoplasm that usually arises in the sebaceous glands of the eyelids (Meibomian and Zeiss glands). It can invasive the eyelid and the cunjunctiva and can metastasize to regional lymph nodes and distant organs. This tumor affects most frequently women after fifty years. The clinical diagnosis is difficult due to atypical presentations simulating benign lesions or other malignant tumors. Furthermore, its surgery is complicated because as we must perform large tumor excision with often difficult eyelid reconstruction. Through our small case series, we describe the clinical describe the clinical features and our management of this tumours.Methods We report six patients presenting with eyelid sebaceous carcinomas: three nodular sebaceous carcinoma, one spreading sebaceous carcinoma and two cases with no safety margin after the initial surgery.Results In all the cases , we completely removed the tumour with safety margin of at least five millimeters and then we reconstructed the eyelid. The diagnosis of sebaceous carcinoma was confirmed by the histological study . Afterward, all our patients benefited from an adjuvant radiotherapy.Conclusion Due to its difficult diagnosis and the delay in treatment, the overall survival rate of sebaceous carcinoma is about 5 to 10 % and can reach 30% for infiltrative forms. The presence of yellowish material within tumour, which can simulate a chalazion, is highly suggestive of sebaceous carcinoma. The best management is the large tumoral excision. In our opinion, the adjuvant radiotherapy must be systematic due to the high grade of malignity. Moreover,the biological behavior of this kind of tumour requires long‐term oncologic following.
Amelanotic conjunctival melanoma: diagnosis and therapeutic management
Authors: SATUE M , BAMBO M , ARA M , EGEA MC , FERNANDEZ‐PEREZ S , HERRERO R , GARCIA‐MARTIN E , DE LA MATA G 
Published: August 2012

AbstractPurpose To present a case report of a patient with atypical presentation of a conjunctival melanoma as an amelanotic tumor.Methods A 82‐year‐old female presenting a large amelanotic tumor in inferior tarsal conjunctiva of the right eye was referred to our hospital. Previous histological analysis diagnosed intraepithelial carcinoma. Imaging digital analysis techniques showed infiltration of the extrinsic ocular muscles but not the scleral tissue. Excisional biopsy was obtained by removing the tumor with free margins and preserving as many unaffected tissue as possible. Amniotic membrane graft was used to cover the surgical injury.Results Histopathological diagnosis confirmed amelanotic conjunctival melanoma. Systemic evaluation with imaging techniques (MRI, TC scan, Ecography…) revealed liver and kidney masses suggestive of metastasis and chemotherapy was prescribed to control the disease. Ophthalmological controls showed remission of tumor for 4 months, but new conjunctival masses appeared. At the present the patient is treated with palliative chemotherapy. Age and systemic status do not allow aggressive therapies in this patient.Conclusion Amelanotic melanoma is an atypical subtype of melanoma lacking of pigmentation. Its macroscopic aspect makes it almost impossible to clinically distinguish this tumor from less aggressive tumors, such as intraepithelial carcinomas. The diagnosis delay makes early treatment difficult in this malignant tumors.
Iris tuberculous granuloma simulating medulloepithelioma
Authors: HADJISTILIANOU T , TOTI P , CORIOLANI G , CERASE A , DE FRANCESCO S , MICHELI L , MENICACCI F , DELUCA A 
Published: August 2012

AbstractPurpose Pediatric ocular granuloma is a rare condition which may result from several causes including trauma, inflammatory diseases, or infectious agents such as fungi, viruses, or mycobacteria. The purpose of this case report is to increase the knowledge about the ophthalmic clinical picture, magnetic resonance imaging findings, histological pattern of a rare case of iris tubercular granuloma occurring in a 4‐year‐old boy.Methods Case reportResults Inflammatory granulomas are usually the expression of systemic inflammatory diseases. Clinical picture may be that of an anterior or posterior uveitis and it may occur with uveal granulomas or episcleral nodules. Our case at diagnosis simulated a medulloepithelioma, both for ophthalmogic and neuroradiologic findings. Pathology following biopsy revealed a tuberculous granulomatous lesion.Conclusion A complete systemic investigation confirmed tuberculous aetiology of this granulomatous lesion.
Never miss uveal lymphoma
Authors: LAVILLE MA , CASSOUX N , MOURIAUX F 
Published: August 2012

AbstractPurpose Primary intraocular lymphomas can mimic many other diseases. The purpose of this presentation is to underline the crucial importance of biopsy.Methods We described two cases of atypical primary uveal lymphomas.Results The first case concerned a 35 year‐old man who presented a painful intraocular tumor with ciliary body infiltration. Enucleation was performed because of high suspicion of melanoma. Pathological findings revealed MALT lymphoma. No other localization was found and no further treatment was done. The second case concerned a 49 year‐old woman who presented blurred vision secondary to a choroidal mass associated with choroidal yellow infiltrates in right eye. An internal chorioretinal biopsy was performed, leading to the diagnosis of diffuse large B cell lymphoma. This high‐grade lymphoma was treated with chemotherapy associated with radiotherapy.Conclusion These two cases illustrate the variety of clinical features of uveal lymphomas, which can mimic choroidal melanoma as in our first case. In our second case, histological findings were not typical and required an aggressive treatment. Biopsy and histological analysis remains the only way to diagnose a primary uveal lymphoma especially in cases of atypical presentation.
A new and standardised method to sample and analyse vitreous biopsies
Authors: VAN GINDERDEUREN R , VAN CALSTER J 
Published: August 2012

AbstractPurpose To investigate and prepare a universal protocol for sampling and analysing vitreous material. Vitreous biopsies are difficult to handle because of the paucity of cells, the gelatinous formation and structure of the vitreous, the low frequency of biopsies and the burden of long‐during, delicate handwork by the technician.Methods After a standardised 23gauge vitrectomy, 50 consecutive vitreous samples were analysed with the Cellient® tissue processor (Hologic). This machine is a fully automatic processor from a specified container with PreservCyt® (fixative fluid) with cells to paraffin. Cytology was compared with fixatives Cytolyt® ( contains a mucolyticum) and Preservcyt®.Results In 96% (48 of 50 cases) sufficient material was found for diagnosis. Cytolyt® wash was necessary in 15% of cases to prevent clotting of the delicate tubes in the Cellient®, this procedure causes a loss of cellular material. Immuno‐histovhemical stainings were equal in quality with both preservatives. Labour hours of processing by technician was diminished by 4, compared with former, not‐standardised techniques.Conclusion A standardised protocol for sampling and handling viteous biopsies by a 23G vitrectomy, fixing in PreservCyt® and processing by the Cellient® gives a superior result in morphology, number of cells, possibility of immuno‐histochemical stainings and technician labour hours.
A case of spontaneously regressed uveal melanoma
Authors: AMIRYAN AG , SAAKYAN S , TATSKOV RA 
Published: August 2012

AbstractPurpose To present a case report of spontaneously regressed uveal melanoma.Methods Complex clinical and instrumental diagnostics was done.Results A patient, 62 y.o. complained of right eye vision absence during last 10 years. Several months ago appeared pain in the eye and this was the cause to visit to the ophthalmologist. The investigation revealed the eye subatrophy, deviation about 20º, moderate conjunctiva injection, corneal edema and dystrophy. Anterior chamber was deep. The iris was infiltrated with heterogeneous pigmented mass. Pupil had irregular form and complicated cataract was present. Eye fundus was not able to visualize. Vision OD=0, OS=1,0. The right eye pressure was decreased. CT‐scanning demonstrated the intraocular mass with calcification spreading into the orbit along the optic nerve sheets. Enucleation with orbitotomy was performed. During the operation the defect of sclera with black mass infiltration around the optic nerve was revealed. The pathomorphologic examination confirmed the spindle cell uveal melanoma with massive zones of calcification in intraocular lesion forming extraocular extension.Conclusion All subatrophy eyes must be examined in details to exclude the intraocular tumors.
Treatment of macular oedema following proton beam therapy for choroidal melanoma with dexamethasone 700µg intravitreal implant
Authors: BAILLIF S , GASTAUD P , CAUJOLLE JP 
Published: August 2012

AbstractPurpose To report the treatment of radiation macular edema following proton beam therapy for choroidal melanoma with dexamethasone 700µg intravitreal implant.Methods Retrospective cases reportResults Five patients were treated with a single injection of intravitreal dexamethasone 700 µg. These patients presented with an uveal melanoma (mean thickness: 4.44 mm, mean highest diameter: 11.14mm) located at 2.58mm (range 0 to 3.5mm) from the macula. Patients were treated with proton beam irradiation (60 Gy cobalt relative biological effectiveness in 4 fractions) 26.75 months (range 16‐43 months) before the occurrence of radiation macular edema. The intravitreal injection of dexamethasone700 µg was performed 5.5 months (1‐14) after the diagnosis of macular edema. Best corrected visual acuity improved for 4 patients (5; 7; 9 and 10 letters), and remained unchanged for 1 patient, over a 5 months follow‐up period. Central retinal thickness decreased for 3 patients (231; 151 and 97 µm) and remained unchanged for 2 patients. Intraocular pressure increased for 1 patient (IOP>25 mmHg at month 3). No other ocular or systemic side effects were noted.Conclusion Radiation macular edema is a common vision threatening complication following proton beam therapy. The available therapeutic options are limited. Intravitreal injections of dexamethasone implant can be of benefit in selected cases.
Identification of mRNAs and proteins specifically associated with transformation in ocular malignant melanoma cells
Authors: COSTAGLIOLA C , SEMERARO F , ROMANO MR , CAPUTO M , DE ROSA MC , TECCE MF 
Published: August 2012

AbstractPurpose Ocular malignant melanoma has several morphological specificity when compared to melanoma tissues from other body regions. To assess and evaluate specific molecular factors of ocular melanoma we decided to screen clinical specimens from patients with melanoma from different tissue and organs, including eye.Methods To identify a number of factors to be analyzed in clinical samples, we selected mRNAs specifically associated with the transformation state of cultured human melanoma cell line A375 This cell line was used as typical reference status of melanoma cells. These cells were then compared to similar cells in which Bag3 mRNA was silenced by transfection with specific siRNA (small interfering for Bag3). This mRNA encodes a protein which has a potent anti‐apoptotic effect and is generally expressed in melanoma cells, being part of the molecular mechanism of their transformation status.Results Microarray techniques took advantage to compare the whole pattern of cellular mRNAs in the two conditions of A375 cells: untreated neoplastic and Bag3 silenced cells. Several mRNA species resulted differentially expressed between the two conditions both as induced or as decreased when comparing the more transformed condition vs. Bag3 silenced cells. We then tested the expression of some of these mRNA and of their encoded proteins within the clinical samples of ocular and non‐ocular melanoma tissues.Conclusion Preliminary results indicated the expression of nephroblastoma overexpressed gene (NOV) as typical of melanoma transformation and of a small pattern of proteins as selectively modulated in ocular melanoma.
Bilateral choroidal metastases as presentation of dissemination of cutaneous malignant melanoma
Authors: FERNANDEZ‐PEREZ S , SATUE M , GARCIA‐MARTIN E , BAMBO M , PRIETO E , DE LA MATA G , PUEYO V , RUIZ‐MORENO O 
Published: August 2012

AbstractPurpose To report the case of a patient with bilateral choroidal mestastases as the first sign of dissemination of cutaneous malignant melanoma.Methods A 47‐year‐old Caucasian male presented to our emergency department with rapid‐onset blurred vision in his right eye (RE). Ophthalmoscopic examination revealed multiple pigmented placoid lesions located in the posterior pole and half periphery of the retina of both eyes and a serous macular detachment in RE. Optical coherence tomography confirmed the macular neurosensory detachment in the RE Since clinical suspicion of choroidal metastases, we referred the patient to the Oncology Department for systematic study. After an exhaustive study, they concluded that it was a dissemination of a cutaneous malignant melanoma with bilateral choroidal metastases, liver and spleen metastases.Results The patient underwent palliative treatment with chemotherapy with improvement of the visual acuity and reduction of the macular neurosensory detachment in the RE. Nevertheless the patient died months later of the diagnosis of cutaneous melanoma dissemination.Conclusion Dissemination of cutaneous melanoma to the choroid is rare and is a major prognostic factor. In general the treatment of ocular metastases is palliative, because the presence of metastases indicates hematogenous spread of tumor. The objectives are therefore to maximize the quality of life and restore or preserve vision.
Massive anterior chamber involvement in advanced retinoblastoma following intra‐arterial chemotherapy
Authors: DE FRANCESCO S , MASTRANGELO D , DE LUCA MC , MENICACCI F , TOTI P , MICHELI L , HADJISTILIANOU T 
Published: August 2012

AbstractPurpose to describe retinoblastoma massive anterior chamber involvement after treatment with intra‐arterial chemotherapy. This condition represents an extremely poor prognostic sign for ocular preservation in patients with retinoblastoma. Therefore, anterior chamber retinoblastoma should be considered an absolute indication for enucleation.Methods Ultrasound biomicroscopy (UBM) was used to document tumour pseudohypopion, cells in the aqueous humor, implanted clusters of cells on the corneal endothelium, iris nodules and lens capsule deposits.Results The UBM data were compared with the histopathologic analysis after enucleation and revealed a significant concordance.Conclusion UBM may represent an important diagnostic tool in retinoblastoma, particularly when the decision about enucleation of the eye, must be made in the absence of histopathologic data.
Posttraumatic periorbital necrotizing fasciitis
Authors: FERNANDEZ‐PEREZ S , HERRERO R , GARCIA‐MARTIN E , DE LA MATA G , SATUE M , BAMBO M , ARA M , EGEA MC , FERNANDEZ‐TIRADO FJ 
Published: August 2012

AbstractPurpose To present the case report of a patient who developed periorbital necrotizing fasciitis following trauma with favorable response.Methods 73 year‐old‐female came to the emergency department after downfall with a trauma in facial region. At the initial examination the patient had a large eyelid swelling in both eyes and a wound that required stitches on the left superciliary region. 5 days later the patient presented to our clinic with general discomfort, fever of 39°C and with slurring, crusty necrotic in the right eyelid, redness and heat in both upper eyelids and in frontal area.Results Because of clinical suspicion of periorbital necrotizing fasciitis, we performed a debridement of the wound in the right upper eyelid, draining the purulent in frontal area, sampling for culture and intravenous treatment with amoxicillin‐clavulanate. In the culture Steptococcus pyogenes isolates. After 15 days of intravenous antibiotic treatment and local cures, the patient was discharged from hospital. One month later, the patient had complete resolution of the lesions with a mild ptosis in the right eye.Conclusion Although this is a disease with a low incidence, it has a high morbidity and mortality if appropriate treatment is not performed. Treatment consists in an immediate and extensive debridement of the affected area with broad‐spectrum antibiotic.
Topography of the human Henle Fiber Layer (HFL) as revealed by imaging and histology
Authors: SJÖSTRAND J , POPOVIC Z 
Published: August 2012

AbstractPurpose To create a model of the topography of the Henle Fibers (HF) connecting photoreceptor cell bodies of the human fovea to the inner retina based on information available in recent imaging and histological studies. Detailed knowledge of the HF is of importance for analysis of connectivity between the outer and inner retina and for evaluation of radial displacement of retinal neurons from their synaptically connected photoreceptors.Methods Reported data of the Henle Fiber Layer (HFL) thickness (HFLt) at different eccentricities along the horizontal meridian of the human fovea from studies with optical coherence tomography (OCT) and histology was used. The radial displacement caused by HF was calculated from data of total displacement within the retina, corrected by data of other displacements obtained from Sjöstrand et al( unpublished).Results A comparison of OCT and histology data showed a similar profile of HFLt vs eccentricity and similar HF topography. The angle of the Henle fibers vs. the external limiting membrane (ELM) was low centrally ranging from a few degrees (deg) to approximately 6 deg at a cone eccentricity of 0.9 mm.Thereafter the angle slowly increased with increasing eccentricity to an angle of 9‐10 deg at cone eccentricity of 1.8 mm . The locations of the maxima of HFLt and projected HF length were at a cone eccentricity of approximately 0.5 mm. A model was constructed displaying the characteristics of the topography of HFL.Conclusion Published data of the topography of HFL fits with a model where the the maximal HFLt is attained at a cone eccentricity where the fiber angle vs ELM still is of low degree and the HF length reaches a maximum.
Stage 1 macular holes of Gass classification illustrated by Optical Coherence Tomography scans: a review of 4 cases
Authors: BENTALEB Z , DENIS P , KODJIKIAN L 
Published: August 2012

AbstractPurpose Gaudric classification, stage 1 corresponds to intraretinal pseudocyst in the inner part of the foveola. The prolongation of the cyst laterally results in a split between the inner retina and the photoreceptor layer and a disruption of the outer retinal layers.Methods Four patients presented with a history of blurred vision and metamorphopsia. OCT examination showed a foveolar serous detachment between pigment epithelium and photoreceptor layer. Six months later, two patients presented lamellar holes and two patients evolved into stage 3 macular holes. In all cases we reported a complete resolution of the foveolar serous detachment . A surgical treatment was performed for three patients.Results OCT scans performed one month after surgery showed a total closure of the macular holes, an increase of visual acuity and a disappearance of the metamorphopsia. The main physiopathological theory formulated about macular holes formation concerns vitreo retinal tractions. As the posterior hyaloid remained attached to the center of the foveola and to the optic disc, displaying a convexity that may indicate the exertion of anteroposterior traction on its vitreous side, Gaudric admits that stage 1a macular hole is the result of antero posterior vitreo retinal tractions. Stage 1 as described by Gass is characterized by a serous foveolar detachment and is a consequence of tangential tractions of the pre foveolar vitreous cortex.Conclusion Our cases seem to be the tomographic illustration of the anatomic description of the stage 1 macular hole proposed by Gass and not mentioned in the tomographic classification proposed by Gaudric suggesting the implicationof tangential traction in macular holes formation.
Dexamethasone intravitreal implant in patients with macular oedema related to branch or central retinal vein occlusion, a retrospective study
Authors: BENZERROUG M , SERRERO M , BREMOND‐GIGNAC D , MILAZZO S 
Published: August 2012

AbstractPurpose To evaluate the anatomic and visual outcomes of one treatment with dexamethasone intravitreal implant (Ozurdex®) in eyes with macular edema secondary to branch or central vein occlusion (BRVO or CRVO) during the first six months.Methods The safety and efficacy of the dexamethasone intravitreal implant (Dex. implant) were evaluated retrospectively from January 2011 to May 2012 in a tertiary referral hospital. CRVO or BRVO diagnosis was confirmed with fluorescein angiography. At baseline, patients received a 0.7 mg dex. implant. They were evaluated at one, three and six months. Best corrected visual acuity (BCVA), optical coherence tomography (OCT) and intraocular pressure (IOP) were collected.Results We included twenty one eyes: 28.5% had a CRVO and 71.5% a BRVO. At baseline, the mean BCVA was 47 letters (ETDRS) (52 in the BRVO group, 22 in the CRVO group). All patients had a macular edema involving the fovea. The mean central retinal thickness (CRT) was 513 micrometer [255‐780]. At one month, 47.5% of eyes had more than three line gains and the mean CRT was 286 µm. Only two eyes had an IOP increase (>25 mmHg). However, at six months, only 17% of eyes had more than three line gains and the mean CRT was 380 µm. IOP remained uncontrolled with medication in one eye.Conclusion The dexamethasone intravitreal implant reduces macular edema and improves visual acuity in patients with either branch retinal vein occlusions or central retinal vein occlusions. However, this efficacy remains transient. A retreament with a dex. implant is necessary for most of the patients.