bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ein Instrument zur Schätzung von Holzernteproduktivitäten mittels der kNN-Methode
Authors: Kostadinov Fabian , Lemm Renato , Thees Oliver 
Published: April 2012

A software tool for the estimation of wood harvesting productivity using the kNN method
For operational planning and management of wood harvests it is important to have access to reliable information on time consumption and costs. To estimate these efficiently and reliably, appropriate methods and calculation tools are needed. The present article investigates whether use of the method of the k nearest neighbours (kNN) is appropriate in this case. The kNN algorithm is first explained, then is applied to two sets of data “combined cable crane and processor” and “skidder”, both containing wood harvesting figures, and thus the estimation accuracy of the method is determined. It is shown that the kNN method's estimation accuracy lies within the same order of magnitude as that of a multiple linear regression. Advantages of the kNN method are that it is easy to understand and to visualize, together with the fact that estimation models do not become out of date, since new data sets can be constantly taken into account. The kNN Workbook has been developed by the Swiss Federal Institute for Forest, Snow and Landscape Research (WSL). It is a software tool with which any data set can be analysed in practice using the kNN method. This tool is also presented in the article.
Integration of FDG- PET/CT into external beam radiation therapy planning
Authors: Beyer T. , Boellaard R. , De Ruysscher D. , Grgic A. , Lee J. A. , Pietrzyk U. , Sattler B. , Schaefer A. , van Elmpt W. , Vogel W. , Oyen W. J. G. , Nestle U. , Thorwarth D. 
Published: April 2012

SummaryThis work addresses the clinical adoption of FDG-PET/CT for image-guided radiation therapy planning (RTP). As such, important technical and methodological aspects of PET/CTbased RTP are reviewed and practical recommendations are given for routine patient management and clinical studies. First, recent developments in PET/CT hardware that are relevant to RTP are reviewed in the context of quality control and system calibration procedures that are mandatory for a reproducible adoption of PET/CT in RTP. Second, recommendations are provided on image acquisition and reconstruction to support the standardization of imaging protocols. A major prerequisite for routine RTP is a complete and secure data transfer to the actual planning system. Third, state-of-the-art tools for image fusion and co-registration are discussed briefly in the context of PET/CT imaging preand post-RTP. This includes a brief review of state-of-the-art image contouring algorithms relevant to PET/CT-guided RTP. Finally, practical aspects of clinical workflow and patient management, such as patient setup and requirements for staff training are emphasized. PET/ CT-guided RTP mandates attention to logistical aspects, patient set-up and acquisition parameters as well as an in-depth appreciation of quality control and protocol standardization. Conclusion: Upon fulfilling the requirements to perform PET/CT for RTP, a new dimension of molecular imaging can be added to traditional morphological imaging. As a consequence, PET/CT imaging will support improved RTP and better patient care. This document serves as a guidance on practical and clinically validated instructions that are deemed useful to the staff involved in PET/CT-guided RTP.
The rare peat moss Sphagnum wulfianum (Sphagnaceae) did not survive the last glacial period in northern European refugia
Authors: Kyrkjeeide Magni Olsen , Hassel Kristian , Flatberg Kjell I. , Stenøien Hans K. 
Published: April 2012

• Premise of the study: Organisms may survive unfavorable conditions either by moving to more favorable areas by means of dispersal or by adapting to stressful environments. Pleistocene glacial periods represent extremely unfavorable conditions for the majority of life forms, especially sessile organisms. Many studies have revealed placements of refugial areas and postglacial colonization patterns of seed plants, but little is still known about areas of long‐term survival and historical migration routes of bryophytes. Given overall differences in stress tolerance between seed plants and bryophytes, it is of interest to know whether bryophytes have survived periods of extreme climatic conditions better then seed plants in northern areas.• Methods: The haploid and rarely spore‐producing peat moss Sphagnum wulfianum is mostly found in areas that were covered by ice during the last glacial maximum. Twelve microsatellite markers were amplified from 43 populations (367 shoots) of this species, and data were analyzed using population genetic diversity statistics, Bayesian clustering methods, and coalescence‐based inference tools to estimate historical and demographic parameters.• Key results: Genetic diversity within populations was low, but populations were highly differentiated, with two main genetic clusters being recognized.• Conclusion: The two main genetic groups have diverged quite recently in the Holocene, and the pattern of genetic variability and structuring gives no support for survival in Scandinavian refugia during the last glacial period in this species. The dispersal ability of this plant thus seems surprisingly high despite its infrequent spore production.
The Influence of Initial Peritoneal Transport Characteristics, Inflammation, and High Glucose Exposure on Prognosis for Peritoneal Membrane Function
Authors: Fernández-Reyes M. José , Bajo M. Auxiliadora , Peso Gloria Del , Ossorio Marta , Díaz Raquel , Carretero Beatriz , Selgas Rafael 
Published: April 2012

♦ Background Fast transport status, acquired with time on peritoneal dialysis (PD), is a pathology induced by peritoneal exposure to bioincompatible solutions. Fast transport has important clinical consequences and should be prevented. ♦ Objective We analyzed the repercussions of initial peritoneal transport characteristics on the prognosis for peritoneal membrane function, and also whether the influence of peritonitis and high exposure to glucose are different according to the initial peritoneal transport characteristics or the moment when such events occur. ♦ Methods The study included 275 peritoneal dialysis patients with at least 2 peritoneal function studies (at baseline and 1 year). Peritoneal kinetic studies were performed at baseline and annually. Those studies consist of a 4-hour dwell with glucose (1.5% during 1981 – 1990, and 2.27% during 1991 – 2002) to calculate the peritoneal mass transfer coefficients of urea and creatinine (milliliters per minute) using a previously described mathematical model. ♦ Results Membrane prognosis and technique survival were independent of baseline transport characteristics. Fast transport and ultrafiltration (UF) failure are reversible conditions, provided that peritonitis and high glucose exposure are avoided during the early dialysis period. The first year on PD is a main determining factor for the membrane's future, and the mass transfer coefficient of creatinine at year 1 is the best functional predictor of future PD history. After 5 years on dialysis, permeability frequently increases, and UF decreases. Icodextrin is associated with peritoneal protection. ♦ Conclusions Peritoneal membrane prognosis is independent of baseline transport characteristics. Intrinsic fast transport and low UF are reversible conditions when peritonitis and high glucose exposure are avoided during the early dialysis period. Icodextrin helps in glucose avoidance and is associated with peritoneal protection.
Therapy for Non-Muscle Invasive Bladder Cancer: HP-NAP
Authors: D'Agostino Daniele , Racioppi Marco , Filianoti Alessio , Di Gianfrancesco Luca , Codolo Gaia , Fassan Matteo , Munari Fabio , Rugge Massimo , D'Elios Mario Milco , de Bernard Marina , Pagano Francesco , Bassi PieFrancesco 
Published: April 2012

Purpose Patients with non-muscle invasive bladder cancer recurrence after 2 induction courses of BCG are eligible for radical cystectomy. So, in the last years research to discover new drugs for the management of non-muscle invasive bladder cancer recurrence after failure of first and second line therapy is ongoing. In accordance to the results obtained with BCG, whose mechanism depends on the induction of the T helper 1 (TH1) immune response, we investigated the activity of a Toll-like receptor (TLR) 2 ligand, named Helicobacter Pylori Neutrophil Activating Protein (HP-NAP), that we recently demonstrated being able of enhancing the differentiation of Th1 cells, both in vitro and in vivo, because of its ability to create an IL-12 enriched milieu. Materials and Methods We show here, in a mouse model of bladder neoplasm implants, that local administration of HP-NAP decreases tumor growth by inducing tumor necrosis. Results The result is joined up with a massive cluster of both CD4+ and CD8+ IFN-γ+ cells, within neoplasm and regional lymph nodes. It is of note that HP-NAP-treated tumors show also a reduced vascularization due to the anti-angiogenic activity of IFN-γ induced by HP-NAP. Conclusions The present study suggests that the activity of HP-NAP against urothelial tumor burden warrants subsequent in vivo studies.
François Maspero et la typographie de l’édition politique1
Authors: Joseph Camille 
Published: April 2012

Les Éditions Maspero, créées à Paris en 1959, fournissent un exemple particulièrement intéressant de la tension très forte qui peut exister entre, d’un côté, un objectif éditorial politique, en l’occurrence anticapitaliste, et, de l’autre, le goût personnel de l’éditeur, François Maspero, pour les dimensions les plus matérielles et « esthétiques » de l’édition. Le décalage entre le contenu des livres et leur habillage visuel fournit une clé pour comprendre la conception de l’édition politique de François Maspero, tout en mettant au jour les contradictions de ce dernier et, peut-être, les contradictions de toute édition politique : le livre politique puise sa légitimité à des sources qui ne sont pas directement politiques. En examinant en particulier le cas de la collection de littérature « Voix », cet article montre comment la présence simultanée d’un goût pour la « pure » typographie et d’une mission politique forte pouvait seulement fonctionner dans la référence à un modèle artisanal d’édition, à l’opposé d’un modèle industriel et capitaliste.
Les ambivalences d’une entreprise de presse gaie : le périodique Gai Pied, de l’engagement au consumérisme
Authors: Pinhas Luc 
Published: April 2012

L’aventure de presse du Gai Pied, mensuel puis hebdomadaire qui se voulait d’informations générales, a joué un rôle majeur, à un moment-clé, dans l’affirmation des gais en France, au tournant des années 1980. Engagé dans une démarche politique et sociale qui dépasse le cadre strict du militantisme, à l’exemple du grand aîné Libération, le journal entend d’abord donner une voix aux gais dans l’espace public et leur permettre de porter un regard spécifique sur le monde en évolution, sans se limiter aux seules problématiques homosexuelles. Les rapports de pouvoir au sein de l’équipe, qui se traduisent par plusieurs crises internes, la difficulté de s’adresser à la communauté gaie dans son ensemble, mais aussi les contraintes liées à une entreprise commerciale et le désinvestissement idéologique qui suit l’élection de François Mitterrand à la présidence de la République le conduisent toutefois, à partir de 1983, à modifier sa stratégie éditoriale. Il sombre alors dans le consumérisme et ne s’adresse plus qu’à une frange de son public, celle qui fréquente assidûment les lieux festifs des grandes villes. L’échec du projet originel, dans son originalité et sa complexité, s’ensuit inéluctablement.
Le sens construit autour de la différenciation pédagogique dans le cadre d’une recherche-action-formation
Authors: Prud’homme Luc , Dolbec André , Guay Marie-Hélène 
Published: April 2012

La différenciation pédagogique se présente actuellement comme unecléfavorisant l’augmentation des taux de réussite scolaire. Or, les exemples de son articulation sur le terrain sont rares et les enseignants s’interrogent sur sa faisabilité. De surcroît, il semble que la formation à l’enseignement comporte des lacunes au regard de la prise en compte de la diversité. C’est dans ce contexte qu’un chercheur s’est engagé dans une démarche de recherche avec onze enseignantes québécoises du préscolaire/primaire afin 1) de comprendre comment des praticiens peuvent concevoir une différenciation en classe et 2) d’approfondir des pistes d’action pouvant soutenir la formation à cette pratique. Les résultats obtenus suggèrent que l’enseignant qui différencie inscrit sa pratique dans un idéal d’interdépendance et d’intercompréhension favorisant la reconnaissance, l’exploitation et la valorisation explicite de la diversité. Il s’ouvre ainsi à la légitimité d’une pluralité de fins et de moyens pour enseigner. Sur le plan de la formation, les résultats suggèrent qu’un travail introspectif favorise une ouverture à la diversité et révèlent cinq critères pédagogiques pouvant orienter le travail du chercheur-formateur dans une démarche de recherche-action-formation : 1) rendre le savoir accessible, 2) favoriser l’engagement, 3) susciter le dialogue cognitif, 4) favoriser la restructuration du savoir et 5) soutenir l’activité réflexive tout au long de la démarche de recherche.
Characterizing Synaptic Vesicle Proteins Using Synaptosomal Fractions and Cultured Hippocampal Neurons
Authors: DiGiovanni Jerome , Sun Tao , Sheng Zu‐Hang 
Published: April 2012

AbstractCloning and characterization of synaptic vesicle proteins and their binding counterparts on the presynaptic plasma membrane have greatly advanced our understanding of the molecular mechanisms involved in the synaptic vesicle cycle and neurotransmitter release. This unit discusses multidisciplinary approaches to characterize proteins from synaptosome‐enriched subcellular fractions and localize them within cultured neurons. The first approach regroups methods used to isolate synaptic vesicles from rat brain synaptosomal preparations, allowing for specific biochemical investigation of synaptic vesicle proteins. The second is a detailed procedure for pre‐embedding immunogold staining and electron microscopic observation, which permits the morphological identification of proteins in individual vesicles at intact synapses. Additionally, this chapter proposes methods for light microscopic examination of hippocampal neurons. It includes procedures for embryonic and postnatal hippocampal neuron culture and describes an immunocytochemical staining protocol used to investigate synaptic vesicle protein localization with respect to other proteins or subcellular structures. Curr. Protoc. Neurosci. 59:2.7.1‐2.7.22. © 2012 by John Wiley & Sons, Inc.
Fountain Flow Cytometry
Authors: Johnson Paul 
Published: April 2012

AbstractFountain Flow Cytometry (FFC) is a simple and inexpensive technology that is adaptable to situations requiring detection and enumeration of cells/organisms at low concentrations, but is limited to particles of relatively high fluorescence intensity. This work presents the basic physics behind the novel scheme Fountain Flow Cytometry employs for the detection of target particles, a hybrid of conventional flow cytometry and video epifluorescence microscopy. The method is based on LED‐induced fluorescence of labeled particles and requires no filtration step. Unlike conventional flow cytometry, the resulting fluorescence is measured with a digital camera as the measured sample flows toward the camera along the optical axis. An automated target particle recognition and enumeration computer program, Biocount, is used to count particles. FFC allows for detection of target particles in transparent and translucent fluids, such as environmental water, blood, and beverages. In addition, FFC can be used for detection of target particles in the presence of high photometric background, including unbound fluorescent dye. This facilitates use of the technique in situations where cells are unwashed. Current applications extend, but are not limited to, particles from µm‐size bacteria to multi‐millimeter‐sized multicellular organisms. Curr. Protoc. Cytom. 60:1.26.1‐1.26.14. © 2012 by John Wiley & Sons, Inc.
Two‐Photon Imaging of the Immune System
Authors: Dzhagalov Ivan L. , Melichar Heather J. , Ross Jenny O. , Herzmark Paul , Robey Ellen A. 
Published: April 2012

AbstractTwo‐photon microscopy is a powerful method for visualizing biological processes as they occur in their native environment in real time. The immune system uniquely benefits from this technology as most of its constituent cells are highly motile and interact extensively with each other and with the environment. Two‐photon microscopy has provided many novel insights into the dynamics of the development and function of the immune system that could not have been deduced by other methods and has become an indispensible tool in the arsenal of immunologists. In this unit, we provide several protocols for preparation of various organs for imaging by two‐photon microscopy that are intended to introduce the new user to some basic aspects of this method. Curr. Protoc. Cytom. 60:12.26.1‐12.26.20. © 2012 by John Wiley & Sons, Inc.
Live Imaging of the Lung
Authors: Thornton Emily E. , Krummel Matthew F. , Looney Mark R. 
Published: April 2012

AbstractLive imaging is critical to determining the dynamics and spatial interactions of cells within the tissue environment. In the lung, this has proven to be difficult due to the motion incurred by ventilation and cardiac contractions. In this chapter, we report protocols for imaging ex vivo live lung slices and the intact mouse lung. Curr. Protoc. Cytom. 60:12.28.1‐12.28.12. © 2012 by John Wiley & Sons, Inc.
Adaptive mixing control with multiple estimators
Authors: Baldi Simone , Ioannou Petros A. , Kosmatopoulos Elias B. 
Published: April 2012

SUMMARYA recently proposed adaptive control scheme with mixing involves the use of precalculated candidate controllers whose output is weighted on the basis of the parameter estimates generated by an online parameter estimator. It has been shown analytically and demonstrated in simulations that adaptive control with mixing exhibits good performance for the regulation case in the presence of modeling errors, disturbances, and time delays. The transient performance, however, depends on the initial conditions of the parameter estimator, and for some initial conditions, the transient oscillations may not be acceptable in certain applications. In this paper, we combine the controller mixing strategy with a multiple parameter estimation architecture plus a hysteresis switching logic selecting the parameter estimates that generate the smaller estimation errors to decide the mixing of the controllers. We show analytically that this approach, referred to as multiestimator adaptive mixing control, achieves stability and stability robustness, and we demonstrate using extensive simulations that the scheme achieves consistently better transient performance than the original adaptive mixing control. In addition, we extend the design and analysis to be applicable to the tracking case where the output is required to track a nonzero reference signal. The stability and performance of the proposed scheme in the presence of unmodeled dynamics and bounded disturbances have also been analyzed and demonstrated using a numerical example. Copyright © 2012 John Wiley & Sons, Ltd.
Adaptive predictive expert control of levels in large canals for irrigation water distribution
Authors: Aguilar José V. , Langarita Pedro , Linares Lorenzo , Rodellar José , Soler Joan 
Published: April 2012

SUMMARYProportional integral (PI) control is the most frequently used method for controlling water levels in irrigation canals. For design purposes, simplified linear models are typically used to describe the series of pools that, separated by control gates, compose the whole canal. Common models are made by series connection of integrator plus time delay or integrator with time delay plus a zero, these models being obtained from the Saint‐Venant equations. However, it is known that hydraulic processes in irrigation canals are strongly nonlinear and present dynamics that are time varying depending on the operating point and the hydraulic conditions of the pool, so that the performance of PI controls may be reduced as the operating point moves far from the nominal design state. This paper presents the application of an adaptive predictive expert control (ADEX) for the distant downstream level control in irrigation canals trying to exploit their adaptive capability to efficiently achieve changes in the setpoint levels and disturbance rejection. Both PI and ADEX controllers are compared in simulation and in real field tests in the control of the end pool of the Canal Imperial de Aragón in Spain. Copyright © 2012 John Wiley & Sons, Ltd.
Acute myeloid leukemia with myelodysplasia‐related changes are characterized by a specific molecular pattern with high frequency of ASXL1 mutations
Authors: Devillier Raynier , Gelsi‐Boyer Véronique , Brecqueville Mandy , Carbuccia Nadine , Murati Anne , Vey Norbert , Birnbaum Daniel , Mozziconacci Marie‐Joëlle 
Published: April 2012

AbstractTo determine whether the distinct and heterogeneous WHO category called “AML with myelodysplasia‐related changes” (MRC‐AML), presents specific molecular alterations we searched for mutations in genes known to be mutated in malignant myeloid diseases. In 48 MRC‐AML patients analyzed, we found 17 mutations in ASXL1 (35%), eight in RUNX1 (17%), seven in TET2 (15%), 12 in IDH (n = 2) or IDH2 (n = 10) (25%), four in DNMT3A (8%), four in NPM1 (8%), and one in FLT3 (2%). Mutations were more frequent in the intermediate cytogenetic (IC) subgroup of 36 patients than in the unfavorable karyotype subgroup, with an average ratio mutations/patients of 1.36 [0–3] vs. 0.33 [0–2] (P < 0.001). Then, we compared these 36 patients with IC MRC‐AML with a control panel of 37 no‐MRC‐AML patients, who had both IC and no dysplasia. IC MRC‐AMLs were associated with higher incidence of ASXL1 mutations (47% vs. 0%, P < 0.001) and lower incidence of DNMT3A (6% vs. 38%, P = 0.001), NPM1 (11% vs. 62%, P < 0.001) and FLT3 (3% vs. 49%, P < 0.001) mutations. No difference was found in the incidence of IDH1/2 or TET2 mutations according to the presence of dysplasia. Complete remission rate after intensive treatment was lower in the MRC‐AML group than in the no‐MRC‐AML group (48% vs. 78%, P = 0.023) and in wild type NPM1 patients (50% vs. 84%, P = 0.009). Our study showed that MRC‐AML as defined in the WHO 2008 classification presents a specific mutation pattern characterized by a high frequency of ASXL1 mutations and a low rate of NPM1, FLT3, and DNMT3A mutations. Am. J. Hematol. 87:659–662, 2012. © 2012 Wiley Periodicals, Inc.