bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longitudinal patterns of autonomic nervous system responding to emotion evocation among children with conduct problems and/or depression
Authors: Pang Karen C. , Beauchaine Theodore P. 
Published: July 2012

AbstractConduct disorder (CD) and depression co‐occur at far greater levels than chance, despite largely separate diagnostic criteria. One potential shared mechanism of this comorbidity is emotion dysregulation, which characterizes both internalizing and externalizing disorders. Previous research demonstrates that respiratory sinus arrhythmia (RSA)—a peripheral biomarker of emotion regulation—is attenuated among children with CD, and among children with depression. However, few studies have examined biomarkers of emotion regulation as a function of heterotypic comorbidity. We evaluated longitudinal patterns of RSA and RSA reactivity to emotion evocation across three annual assessments among 207 children diagnosed at ages 8–12 years with CD (n = 30), depression (n = 28), comorbid CD and depression (n = 80), or no psychiatric condition (n = 69). Using continuous symptom counts as predictors, Depression × CD interactions were observed for both Time 1 resting RSA and Time 1 RSA reactivity. CD, depression, and their interaction were all associated with low resting RSA at Time 1. In addition, concurrently elevated CD and depression scores predicted the greatest RSA reactivity to emotion evocation. Psychopathology scores were unrelated to developmental changes in RSA and RSA reactivity over time. © 2012 Wiley Periodicals, Inc. Dev Psychobiol 55: 698–706, 2013.
Assessment of thought–shape fusion: Initial validation of a short version of the trait thought–shape fusion scale
Authors: Coelho Jennifer S. , Baeyens Céline , Purdon Christine , Shafran Roz , Roulin Jean‐Luc , Bouvard Martine 
Published: July 2012

AbstractObjective:Thought–shape fusion (TSF) is a cognitive distortion that has been linked to eating pathology. Two studies were conducted to further explore this phenomenon and to establish the psychometric properties of a French short version of the TSF scale.Method:In Study 1, students (n = 284) completed questionnaires assessing TSF and related psychopathology. In Study 2, the responses of women with eating disorders (n = 22) and women with no history of an eating disorder (n = 23) were compared.Results:The French short version of the TSF scale has a unifactorial structure, with convergent validity with measures of eating pathology, and good internal consistency. Depression, eating pathology, body dissatisfaction, and thought‐action fusion emerged as predictors of TSF. Individuals with eating disorders have higher TSF, and more clinically relevant food‐related thoughts than do women with no history of an eating disorder.Discussion:This research suggests that the shortened TSF scale can suitably measure this construct, and provides support for the notion that TSF is associated with eating pathology. © 2012 by Wiley Periodicals, Inc. (Int J Eat Disord 2013)
Parent reported inattention and hyperactivity/impulsivity as predictor of long‐term weight loss after inpatient treatment in obese adolescents
Authors: van Egmond‐Froehlich Andreas , Claußnitzer Gerd , Dammann Dirk , Eckstein Elisabeth , Bräuer Wolfgang , de Zwaan Martina 
Published: July 2012

AbstractObjective:The long‐term success of treatment for pediatric obesity is often unsatisfactory and variable. We aim to elucidate the influence of inattention and hyperactivity/impulsivity on weight loss after inpatient treatment for adolescent obesity.Method:We included 13–17 year old obese participants treated in three inpatient multidisciplinary treatment centers. At the beginning and end of treatment and at one year follow‐up weight and height were measured. Inattention and hyperactivity/impulsivity was assessed with the hyperactivity/inattention (HI) subscale of the parent‐rated Strengths and Difficulties Questionnaire. General linear models were used with the standard deviation scores of the &&num;4;body mass index (BMI‐SDS) as dependent variable.Results:Totally, 253 participants were included (65% female, age: 15.3 ± 1.4 years, baseline BMI‐SDS: 3.13 ± 0.38 kg/m2). HI scores were associated with long‐term (p < .001) but not short term (ns) weight loss.Discussion:This indicates that inattention and hyperactivity/impulsivity is associated with reduced long‐term weight loss success in adolescent inpatients. © 2012 by Wiley Periodicals, Inc. (Int J Eat Disord 2013)
Genetic analysis of Black Tiger shrimp (Penaeus monodon) across its natural distribution range reveals more recent colonization of Fiji and other South Pacific islands
Authors: Waqairatu Salote S. , Dierens Leanne , Cowley Jeff A. , Dixon Tom J. , Johnson Karyn N. , Barnes Andrew C. , Li Yutao 
Published: July 2012

AbstractThe Black Tiger shrimp (Penaeus monodon) has a natural distribution range from East Africa to the South Pacific Islands. Although previous studies of Indo‐Pacific P. monodon have found populations from the Indian Ocean and Australasia to differ genetically, their relatedness to South Pacific shrimp remains unknown. To address this, polymorphisms at eight shared microsatellite loci and haplotypes in a 418‐bp mtDNA‐CR (control region) sequence were examined across 682 P. monodon from locations spread widely across its natural range, including the South Pacific islands of Fiji, Palau, and Papua New Guinea (PNG). Observed microsatellite heterozygosities of 0.82–0.91, allele richness of 6.85–9.69, and significant mtDNA‐CR haplotype variation indicated high levels of genetic diversity among the South Pacific shrimp. Analysis of microsatellite genotypes using a Bayesian STRUCTURE method segregated Indo‐Pacific P. monodon into eight distinct clades, with Palau and PNG shrimp clustering among others from Southeast Asia and eastern Australia, respectively, and Fiji shrimp clustering as a distinct group. Phylogenetic analyses of mtDNA‐CR haplotypes delineated shrimp into three groupings, with shrimp from Fiji again being distinct by sharing no haplotypes with other populations. Depending on regional location, the genetic structures and substructures identified from the genotyping and mtDNA‐CR haplotype phylogeny could be explained by Metapopulation and/or Member–Vagrant type evolutionary processes. Neutrality tests of mutation‐drift equilibrium and estimation of the time since population expansion supported a hypothesis that South Pacific P. monodon were colonized from Southeast Asia and eastern Australia during the Pleistocene period over 60,000 years ago when land bridges were more expansive and linked these regions more closely.
Complex‐Formation Ability of Salicylaldehyde Thiosemicarbazone towards ZnII, CuII, FeII, FeIII and GaIII Ions
Authors: Enyedy Éva A. , Zsigó Éva , Nagy Nóra V. , Kowol Christian R. , Roller Alexander , Keppler Bernhard K. , Kiss Tamás 
Published: July 2012

AbstractThe stoichiometry and stability of copper(II), zinc(II), iron(II)/(III) and gallium(III) complexes of salicylaldehyde thiosemicarbazone (STSC, H2L) have been determined by pH potentiometry, UV/Vis spectrophotometry, and 1H NMR and EPR spectroscopy in aqueous solution (with 30 % DMSO), together with the characterization of the proton dissociation processes. Mono‐ and bis‐ligand complexes in different protonation states were identified for FeII, FeIII and GaIII, whereas CuII and ZnII ions only form complexes with a 1:1 metal/ligand ratio. The coordination mode in the complex [Zn2(HL)(L)(OAc)EtOH] was confirmed by X‐ray diffraction. The metal‐binding ability of STSC at physiological pH is in the following order: GaIII < ZnII < FeII < FeIII < CuII. GaIII–STSC complexes show unambiguously higher stability, whereas FeII–STSC species show significantly lower stability relative to the corresponding α(N)‐pyridyl thiosemicarbazones like 2‐formylpyridine thiosemicarbazone or Triapine. Furthermore, the fluorescence properties of the ligand were investigated in aqueous solution, and their changes caused by complexation with GaIII and ZnII were studied.
Hypoxic priming of mESCs accelerates vascular‐lineage differentiation through HIF1‐mediated inverse regulation of Oct4 and VEGF
Authors: Lee Sae‐Won , Jeong Han‐Kyul , Lee Ji‐Young , Yang Jimin , Lee Eun Ju , Kim Su‐Yeon , Youn Seock‐Won , Lee Jaewon , Kim Woo Jean , Kim Kyu‐Won , Lim Jeong Mook , Park Jong‐Wan , Park Young‐Bae , Kim Hyo‐Soo 
Published: July 2012

AbstractHypoxic microenvironment plays an important role in determining stem cell fates. However, it is controversial to which direction between self‐renewal and differentiation the hypoxia drives the stem cells. Here, we investigated whether a short exposure to hypoxia (termed ‘hypoxic‐priming’) efficiently directed and promoted mouse embryonic stem cells (mESCs) to differentiate into vascular‐lineage. During spontaneous differentiation of embryoid bodies (EBs), hypoxic region was observed inside EB spheroids even under normoxic conditions. Indeed, hypoxia‐primed EBs more efficiently differentiated into cells of vascular‐lineage, than normoxic EBs did. We found that hypoxia suppressed Oct4 expression via direct binding of HIF‐1 to reverse hypoxia‐responsive elements (rHREs) in the Oct4 promoter. Furthermore, vascular endothelial growth factor (VEGF) was highly upregulated in hypoxia‐primed EBs, which differentiated towards endothelial cells in the absence of exogenous VEGF. Interestingly, this differentiation was abolished by the HIF‐1 or VEGF blocking. In vivo transplantation of hypoxia‐primed EBs into mice ischemic limb elicited enhanced vessel differentiation. Collectively, our findings identify that hypoxia enhanced ESC differentiation by HIF‐1‐mediated inverse regulation of Oct4 and VEGF, which is a novel pathway to promote vascular‐lineage differentiation.
Use of the bioaccumulation factor to screen chemicals for bioaccumulation potential
Authors: Costanza Jed , Lynch David G. , Boethling Robert S. , Arnot Jon A. 
Published: July 2012

Abstract
The fish bioconcentration factor (BCF), as calculated from controlled laboratory tests, is commonly used in chemical management programs to screen chemicals for bioaccumulation potential. The bioaccumulation factor (BAF), as calculated from field-caught fish, is more ecologically relevant because it accounts for dietary, respiratory, and dermal exposures. The BCFBAF™ program in the U.S. Environmental Protection Agency's Estimation Programs Interface Suite (EPI Suite™ Ver 4.10) screening-level tool includes the Arnot-Gobas quantitative structure–activity relationship model to estimate BAFs for organic chemicals in fish. Bioaccumulation factors can be greater than BCFs, suggesting that using the BAF rather than the BCF for screening bioaccumulation potential could have regulatory and resource implications for chemical assessment programs. To evaluate these potential implications, BCFBAF was used to calculate BAFs and BCFs for 6,034 U.S. high- and medium-production volume chemicals. The results indicate no change in the bioaccumulation rating for 86% of these chemicals, with 3% receiving lower and 11% receiving higher bioaccumulation ratings when using the BAF rather than the BCF. All chemicals that received higher bioaccumulation ratings had log KOW values greater than 4.02, in which a chemical's BAF was more representative of field-based bioaccumulation than its BCF. Similar results were obtained for 374 new chemicals. Screening based on BAFs provides ecologically relevant results without a substantial increase in resources needed for assessments or the number of chemicals screened as being of concern for bioaccumulation potential. Environ. Toxicol. Chem. 2012; 31: 2261–2268. © 2012 SETAC
Oxytetracycline interactions at the soil–water interface: Effects of environmental surfaces on natural transformation and growth inhibition of Azotobacter vinelandii
Authors: Goetsch Heather E. , Mylon Steven E. , Butler Shane , Zilles Julie L. , Nguyen Thanh H. 
Published: July 2012

Abstract
The mechanism of oxytetracycline (OTC) adsorption to a silty clay loam soil was investigated using sorption isotherm experiments, Fourier transform infrared spectroscopy (FTIR), and X-ray diffraction spectroscopy (XRD). Sorption data fit well to a cation-exchange capacity sorption model. Spectroscopic data indicate that the interactions between oxytetracycline and silty clay loam soil were primarily through electrostatic interactions between the protonated dimethylamino group of OTC and the negatively charged moieties on the surface of the soil. Based on XRD results, OTC adsorption appeared to inhibit the ethylene glycol solvation of the expandable clay minerals, suggesting that OTC had diffused into the clay interlayer space. The presence of adsorbed OTC did not significantly affect the transformation frequency of the soil bacterium Azotobacter vinelandii with plasmid DNA (soil alone 3 × 106 ± 4 × 106 and soil with adsorbed OTC 4 × 106 ± 0.5 × 106). Growth was inhibited by adsorbed OTC, although a greater mass of adsorbed OTC was required to achieve the same degree of inhibition as the system of dissolved OTC alone. These results suggest that the interactions of tetracyclines at the soil–water interface will affect the growth of sensitive microorganisms in soil microbial communities. Environ. Toxicol. Chem. 2012; 31: 2217–2224. © 2012 SETAC
Transcriptional responses in Enchytraeus albidus (Oligochaeta): Comparison between cadmium and zinc exposure and linkage to reproduction effects
Authors: Novais Sara C. , De Coen Wim , Amorim Mónica J.B. 
Published: July 2012

Abstract
Metal ecotoxicity to soil organisms (for example, in enchytraeids) has been addressed mainly by assessing effects on survival and reproduction, but very little is known about the underlying molecular mechanisms of responses. The main purpose of the present study was to assess and compare the transcriptional responses of Enchytraeus albidus to an essential (Zn) and a nonessential (Cd) metal. Exposure was performed with two concentrations with a known effect on reproduction (effective concentration for 50% [EC50] and 90% [EC90]) at three time points (2, 4, and 8 d). Results showed that transcriptional responses were influenced by exposure duration but, independently of that, the mechanisms of response to Cd and Zn were consistently different. Both metals affected pathways related to the regulation of gene expression, calcium homeostasis, and cellular respiration. Mechanisms of toxicity that were exclusively associated with Cd exposures were the inhibition of DNA repair and the impairment of ubiquitin-mediated proteolysis. The microarray for E. albidus was a useful tool for detecting molecular pathways affected by metal exposures. Transcriptional responses strongly correlated with known mechanisms of Cd and Zn responses in other organisms, suggesting cross-species conserved mechanisms of action. It should be highlighted not only that the authors could retrieve mechanistic information but also that genes responded within 2 to 8 d of exposure. This represents an additional advantage of using such molecular endpoints as a complement to the traditional, more time-consuming endpoints. Environ. Toxicol. Chem. 2012; 31: 2289–2299. © 2012 SETAC
Influence of humic acid on the uptake of aqueous metals by the killifish Fundulus heteroclitus
Authors: Dutton Jessica , Fisher Nicholas S. 
Published: July 2012

Abstract
The role of humic acids, over a concentration range of 0 to 20 mg L−1, was investigated in the uptake of three metals (Cd, Cr, and Hg—as both inorganic Hg [Hg(II)] and methylmercury [MeHg]) and a metalloid (As) from the aqueous phase by the killifish (Fundulus heteroclitus). Cadmium uptake showed no relationship with humic acid concentration, whereas Cr, Hg(II), and MeHg uptake showed an inverse relationship, and As uptake increased with increasing humic acid concentration. Concentration factors were &gt;1 for Cd, Hg(II), and MeHg at all humic acid concentrations, indicating killifish were more enriched in the metal than the experimental media, whereas As and Cr generally had concentration factors &lt;1 at the end of a 72-h exposure. The uptake of As and Cr reached steady state within the 72-h exposure, whereas uptake of Cd, Hg(II), and MeHg did not. Uptake rate constants (kus; ml g−1 d−1) were highest for MeHg (91–3,936), followed by Hg(II), Cd, and Cr, and lowest for As (0.17–0.29). Dissection data revealed that the gills generally had the highest concentration of all metals under all humic acid treatments. The present study concludes that changes in humic acid concentration can influence the accumulation of aqueous metals in killifish and should be considered when modeling metal bioaccumulation. Environ. Toxicol. Chem. 2012; 31: 2225–2232. © 2012 SETAC
Dissolved organic carbon modulates the effects of copper on olfactory-mediated behaviors of chinook salmon
Authors: Kennedy Christopher J. , Stecko Pierre , Truelson Bob , Petkovich David 
Published: July 2012

Abstract
The modulation of Cu effects on olfactory-mediated behaviors by dissolved organic carbon (DOC) and Fe was examined in juvenile chinook salmon. Chinook were exposed to several concentrations of Cu, DOC, and Fe alone or in combination in a flow-through system for either 4 d (acute exposure) or 14 d (subchronic exposure) and tested for their ability to detect and avoid the odorant L-histidine in an avoidance/preference trough assay. In both acute and subchronic exposures, Cu inhibited the ability of fish to detect this amino acid in a concentration-dependent manner, and Cu toxicity (olfactory inhibition) decreased with increasing DOC concentration. In both acute and subchronic experiments including DOC, Cu-induced olfactory inhibition decreased in a linear fashion with increasing DOC concentration, although the modulation was lower in subchronic exposures. The protective effect of DOC on Cu olfactory inhibition was reduced only slightly in the presence of Fe, indicating that other metals can potentially affect the modulation of the olfactory inhibition of Cu through competition for DOC binding sites. The results of the present study clearly show the amelioration by DOC of the effects of Cu on juvenile chinook salmon olfaction at a behavioral level. These data further indicate that DOC concentrations should be considered when evaluating the potential impact of Cu on fish olfaction. Environ. Toxicol. Chem. 2012; 31: 2281–2288. © 2012 SETAC
Effects of road deicer (NaCl) and amphibian grazers on detritus processing in pond mesocosms
Authors: Van Meter Robin J. , Swan Christopher M. , Trossen Carrie A. 
Published: July 2012

Abstract
Road deicers have been identified as potential stressors in aquatic habitats throughout the United States, but we know little regarding associated impacts to ecosystem function. A critical component of ecosystem function that has not previously been evaluated with respect to freshwater salinization is the impact on organic matter breakdown. The purpose of this study was to evaluate cumulative effects of road deicers and tadpole grazers on leaf litter breakdown rate (g d−1) and microbial respiration (mg O2 g leaf−1 h−1). To test this interaction, in May 2008 the authors added dry leaf litter (Quercus spp.) to forty 600-L pond mesocosms and inoculated each with algae and zooplankton. In a full-factorial design, they manipulated a realistic level of road salt (ambient or elevated at 645 mg L−1 Cl−) and tadpole (Hyla versicolor) presence or absence. The elevated chloride treatment reduced microbial respiration by 24% in the presence of tadpoles. The breakdown of leaf litter by tadpoles occurred 9.7% faster under ambient chloride conditions relative to the elevated chloride treatment. Results of the present study suggest that the microbial community is directly impacted by road deicers and heavy tadpole grazing under ambient conditions limits microbial capacity to process detritus. Road salts and tadpoles interact to limit microbial respiration, but to a lesser extent leaf mass loss rate, thereby potentially restricting energy flow from detrital sources in pond ecosystems. Environ. Toxicol. Chem. 2012; 31: 2306–2310. © 2012 SETAC
Response of biomarkers in amphibian larvae to in situ exposures in a fruit-producing region in North Patagonia, Argentina
Authors: Rosenbaum Enrique A. , Duboscq Leonardo , Soleño Jimena , Montagna Cristina M. , Ferrari Ana , Venturino Andrés 
Published: July 2012

Abstract
The authors evaluated biomarker responses in caged larvae of the amphibian Rhinella arenarum in water channels during fruit production season and compared them with those elicited by a transient exposure to azinphos methyl (AzM) (0.02–2 mg/L; 4 h), the main pesticide applied in the Alto Valle region, Patagonia, Argentina, taking into account the maximum environmental concentration detected in superficial water (22.5 µg/L). The traditional biomarkers of organophosphate exposure, acetylcholinesterase (AChE) and carboxylesterase, were inhibited in tadpoles after one week of exposure in channels potentially receiving pesticide drift, whereas the antioxidant glutathione (GSH) and the detoxifying activity of GSH S-transferase (GST) were induced. In a two-week monitoring study, AChE activity was induced in larvae exposed at the agricultural site, and carboxylesterase showed an inhibition followed by return to control values, suggesting an exposure–recovery episode. Antioxidant glutathione levels were first depleted and then surpassed control levels, whereas GST activity was continuously induced. These responses were mimicked in the laboratory by 2 mg/L AzM-pulse exposure, which notably exceeds the expected environmental concentrations. The results draw attention to the complexity of responses after pesticide exposure, strongly depending on exposure time-concentration and recovery periods, among other possible factors, and support the necessity of the integrated use of biomarkers to assess exposure episodes in agricultural areas. Environ. Toxicol. Chem. 2012; 31: 2311–2317. © 2012 SETAC
Effects of environmental lead contamination on cattle in a lead/zinc mining area: Changes in cattle immune systems on exposure to lead in vivo and in vitro
Authors: Ikenaka Yoshinori , Nakayama Shouta M.M. , Muroya Taro , Yabe John , Konnai Satoru , Darwish Wageh Sobhy , Muzandu Kaampwe , Choongo Kennedy , Mainda Geoffrey , Teraoka Hiroki , Umemura Takashi , Ishizuka Mayumi 
Published: July 2012

Abstract
The Republic of Zambia is rich in mineral resources, such as zinc (Zn) and lead (Pb), and mining is a key industry in Zambia. A previous study of Pb pollution in Kabwe, one of the main mining areas, found that soil was contaminated with high levels of toxic metals over a substantial area. In the present study, the authors focus on toxic metal pollution in cattle, one of the most important domestic animals in Zambia. Blood samples from cattle in Kabwe and a control area (Lusaka) were tested for toxic metal content. They also measured mRNA expression of metal-responsive proteins and cytokines in white blood cells using real-time reverse transcriptase polymerase chain reaction. In the present in vitro study, The authors cultured peripheral blood mononuclear cells (PBMCs) from cattle, exposing them to Pb acetate for 24 h and analyzing mRNA expression of metal-responsive proteins and selected cytokines. Lead concentrations in cattle blood from Kabwe were significantly greater than those from Lusaka, as were the mRNA expressions of metallothionein-2 (MT-2), tumor necrosis factor-α (TNF-α), interferon-γ (IFN-γ), interleukin-1β (IL-1β), IL-6, and inducible nitric oxide synthase (iNOS). The present in vitro study demonstrated that Pb exposure led to an increase in the expressions of MT-2, TNF-α, IL-1β, and iNOS, similar to those found in vivo. These results indicate the possibility of immune system modulations in cattle from the Kabwe area. Environ. Toxicol. Chem. 2012; 31: 2300–2305. © 2012 SETAC