bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endometrial glandular and stromal breakdown, part 4: Cytomorphology of “condensed cluster of stromal cells including a light green body”
Authors: Norimatsu Yoshiaki , Kawai Miho , Kamimori Akira , Yuminamochi Tsutomu , Ohsaki Hiroyuki , Yanoh Kenji , Kawanishi Namiki , Kobayashi Tadao K. 
Published: November 2010

AbstractThis study was undertaken to clarify the origin of the chromophilic substance that stained in light green [light green body (LGB)] was observed in the condensed cluster of stromal cells and in the background of endometrial glandular and stromal breakdown cases. The material consists of cytologic smears of endometrial glandular and stromal breakdown (EGBD) from 58 samples in which a histopathological diagnosis was subsequently obtained by endometrial curettage.The following parameters were examined. (1) The occurrence and number of a LGB. As for the “condensed cluster of stromal cells,” “condensed cluster of stromal cells including LGB” and “LBG in background,” it was observed with all fields on one slide per case. When it was observed more than one in a preparation, the occurrence was determined and the number was also calculated. (2) Immunostaining of paraffin‐embedded tissue sections and cytologic preparations.
The occurrence of “condensed cluster of stromal cells,” “condensed cluster of stromal cells including LGB” and “LGB in background” was 100%, 44.8% and 91.4%, respectively. The number of “condensed cluster of stromal cells,” “condensed cluster of stromal cells including LGB” and “LGB in background” was 21.2, 2.0 and 4.0, respectively.
When histological sections from paraffin‐embedded tissue were tested by immunocytochemistry, “LGB” stained weakly or negative for fibrinogen, whereas it stained positively for CD31 and factor VIII. In addition, it stained positively for CD42b.
When cytologic preparation was tested by immunocytochemistry, “LGB” stained positively for CD31, factor VIII and CD42b. However, as for the fibrinogen, quantitative and qualitative evaluation of the immunocytochemical stain was not feasible due to extensive nonspecific reaction in the whole preparation.
The “LGB” in EGBD cases proved to be a thrombus mostly made up by platelets, and it seemed that the recognition of the LGB showed to be a useful cytomorphological criterion for an always more accurate diagnosis of EGBD cases. Diagn. Cytopathol. 2012. © 2010 Wiley Periodicals, Inc.
Diagnostic utility of vitreous humor fluid cytology for intraocular sarcoidosis: A clinicopathologic study of 7 cases
Authors: Kinoshita Yuichi , Takasu Kosho , Adachi Yasuhi , Yuri Takashi , Matsunaga Shiho , Nagumo Sachiko , Shikata Nobuaki 
Published: November 2010

AbstractSarcoidosis is an enigmatic multisystem sarcoid disease occurring mainly in the lungs, skin, and eyes. Sarcoidosis of the eyes (intraocular sarcoidosis) has been proposed as one of the causes of uveitis. We carried out a clinicopathological study involving seven patients with intraocular sarcoidosis based on vitreous humor fluid cytology.In the seven patients, the sex ratio was 4:3, and the average age at onset was 65.6‐years‐old. Vitreous opacity was noted in all patients and snowball‐like vitreous opacity in 85.7%. Nerve papilloedema was noted in 42.9%. Diagnostic vitrectomy and adrenocortical hormone therapy were performed in all patients. As a result, the symptoms were improved. Vitreous perfusion fluid cytology revealed multinucleate giant cells in 85.7% and lymphocytes and epithelioid cells in all cases.Cytological diagnosis is not included in the current criteria for sarcoidosis, but its value in the diagnosis of intraocular sarcoidosis was suggested based in present study. Diagn. Cytopathol. 2012. © 2010 Wiley Periodicals, Inc.
Squash cytology of subependymal giant cell astrocytoma: Report of four cases with brief review of literature
Authors: Jaiswal Sushila , Vij Mukul , Jaiswal Awadhesh Kumar , Srivastava Arun Kumar , Behari, Sanjay 
Published: November 2010

AbstractSquash cytology description of subependymal giant cell astrocytoma (SEGA) is poorly described in the literature. Objective of this study is to study the cytomorphological features of SEGA in squash smears. Introperative squash smear preparations of four patients of SEGA diagnosed from 2004 to 2010 were reviewed. One to two millimetres of the biopsy material was crushed between two glass slides to make a thin film and fixed in 95% alcohol and stained by haematoxylin and eosin (H&E). Smear cytology diagnoses were correlated with clinical, radiological data and finally with histopathological findings of the tumor specimen.We had four cases (all males; age range 11–15 years; mean age 12.5 years). All tumors were located in the lateral ventricles. The cytological smears in all four cases were cellular and display pleomorohic binucleate to multinucleate tumor cells disposed in cohesive clusters, groups or lying singly. Elongated spindle shaped and strap cells with long thick cell processes were also identified. On squash smears, SEGA can be diagnosed when cytomorphological features are correlated with clinical and radiological finding. The distinct cytomorphological features are cellular smears comprising of pleomorohic binucleate to multinucleate tumor cells with round to polygonal eccentrically placed mildly anisomorphic nuclei, prominent nucleoli, and abundant eosinophilic glassy cytoplasm. Astrocytic fibrillary background, endothelial proliferation, mitosis, and necrosis are lacking. Diagn. Cytopathol. 2010. © 2010 Wiley‐Liss, Inc.
The combined influence of genetic factors and sedentary activity on body mass changes from adolescence to young adulthood: the National Longitudinal Adolescent Health Study
Authors: Graff M. , North K. E. , Monda K. L. , Lange E. M. , Lange L. A. , Guo G. , Gordon‐Larsen P. 
Published: November 2010

AbstractBackgroundAn increase in sedentary activities is likely a major contributor to the rise in obesity over the last three decades. Little research has examined interactions between genetic variants and sedentary activity on obesity phenotypes. High levels of sedentary activity during adolescence may interact with genetic factors to influence body mass changes between adolescence and young adulthood, a high risk period for weight gain.MethodsIn the National Longitudinal Study of Adolescent Health, siblings and twin pairs (16.5 ± 1.7 years) were followed into young adulthood (22.4 ± 1.8 years). Self‐reported screen time (TV, video, and computer use in h/week) and body mass index (kg/m2), calculated from measured height and weight at adolescence and at young adulthood, were available for 3795 participants. We employed a variance component approach to estimate the interaction between genotype and screen time for body mass changes. Additive genotype‐by‐screen time interactions were assessed using likelihood‐ratio tests. Models were adjusted for race, age, sex, and age‐by‐sex interaction.ResultsThe genetic variation in body mass changes was significantly larger in individuals with low ($\sigma_{{{\rm G}}} = 27.59 \pm 1.58$) compared with high ($\sigma_{{{\rm G}}} = 18.76 \pm 2.59$) levels of screen time (p < 0.003) during adolescence.ConclusionsOur findings demonstrate that sedentary activities during adolescence may interact with genetic factors to influence body mass changes between adolescence and young adulthood. Accounting for obesity‐related behaviours may improve current understanding of the genetic variation in body mass changes. Copyright © 2010 John Wiley & Sons, Ltd.
Heat makes a difference in activity‐based anorexia: A translational approach to treatment development in anorexia nervosa
Authors: Cerrato Maria , Carrera Olaia , Vazquez Reyes , Echevarría Enrique , Gutierrez Emilio 
Published: November 2010

AbstractObjective:To test the effect of raising ambient temperature (AT) on activity‐based anorexia (ABA) and to extend to female rats previous findings reported in male animals.Method:Two studies are reported in which female rats were submitted to food restriction and free access to an activity wheel either separately or in combination under changing (21–32°C) or constant AT (21°C).Results:Warming ABA animals reversed running activity, preserved food‐intake, and enabled female rats to recover from acute weight loss. Moreover, sedentary food‐restricted warmed rats maintained a body weight equivalent to the levels of animals housed at standard AT in spite of 20% reduced food‐intake.Discussion:The replication on female rats corroborates the effect previously reported for males, which is indicative of the robust effect of AT in recovering rats from ABA. The findings reported here represent strong preclinical evidence in favor of heat supply as a useful adjunctive component for the treatment of anorexia nervosa (AN). © 2010 by Wiley Periodicals, Inc. (Int J Eat Disord 2012)
The response of carbon dioxide exchange to manipulations of Sphagnum water content in an ombrotrophic bog
Authors: Adkinson A. C. , Humphreys E. R. 
Published: November 2010

AbstractSphagnum mosses contribute to the long‐term carbon sequestration of many northern peatlands. Variability in both Sphagnum and peatland CO2 exchange is strongly influenced by water availability, but there is limited work examining the links between these relationships, particularly in a field setting. In this study, the effects of varying moss water content (WC), through precipitation exclusion (PE) and water addition (WA) treatments, on net ecosystem CO2 exchange (NEE) and component fluxes were assessed during a single growing season at Mer Bleue bog, a temperate ombrotrophic peatland. CO2 exchange by Sphagnum, vascular plants, and the total ecosystem were measured in situ using chambers. On average, the hummock species of Sphagnum dominant at the site maintained WCs within the optimal range for photosynthesis in all treatments and no treatment effect on CO2 exchange was observed. However, in a few samples associated with below optimal WCs, Sphagnum photosynthesis was reduced. Water table (WT) depth and direct precipitation were both important controls on Sphagnum WC, suggesting that frequent small rain events may be important in maintaining Sphagnum WC. This study only explored one variable associated with drought conditions (i.e. water availability through intercepted precipitation). Consequently, further study is necessary to assess the relative importance of deep WT positions and high temperatures on moss WC. Nevertheless, the results of this study suggest that decreased Sphagnum photosynthesis has the potential to influence peatland CO2 uptake at Mer Bleue in drought years, but only if moss WC is reduced below the optimal range. Copyright © 2010 John Wiley & Sons, Ltd.
Water use patterns of three species in subalpine forest, Southwest China: the deuterium isotope approach
Authors: Xu Qing , Li Harbin , Chen Jiquan , Cheng Xiaoli , Liu Shirong , An Shuqing 
Published: November 2010

AbstractDetermination of water sources of plant species in a community is critical for understanding the hydrological processes and their importance in ecosystem functions. Such partitioning of plant xylem water into specific sources (i.e. precipitation, groundwater) can be achieved by analysing deuterium isotopic composition (δD) values for source waters. A subalpine dark coniferous forest in southwestern China was selected to examine water use strategies of three key species. Our objectives are to: (1) determine contributions of rainfall and groundwater to soil and xylem water and (2) examine effects of rain, days after rain, soil depth, and species on such contributions. We found that the three species tend to have different, but complementary water use patterns. The dominant canopy tree of Abies faxoniana relies primarily on groundwater (i.e. 66–96% of its water sources) and does not change its dependency on groundwater between seasons. In contrast, the midstory Betula utilis and the understory Bashania fangiana depend predominantly on rainwater (i.e. 13–94% and 32–93%, respectively), and tend to use water opportunistically, switching to groundwater as the main source under stressful conditions during the dry season. The complementary and, for some species, shifting water use strategies and the lack of dependency on rainwater by the foundation species of the subalpine coniferous forest ecosystem should act together to promote species co‐existence and maintain community resiliency under potentially increasing water stress caused by climate change. Copyright © 2010 John Wiley & Sons, Ltd.
The IgH 3′ regulatory region and its implication in lymphomagenesis
Authors: Vincent‐Fabert Christelle , Fiancette Rémi , Cogné Michel , Pinaud Eric , Denizot Yves 
Published: November 2010

AbstractThe 3′ regulatory region (3′RR) located downstream of the IgH gene is the master element that controls class switch recombination and sustains high‐level transcription at the plasma‐cell stage. This latter role suggests that the 3′RR may be involved in oncogene deregulation during the frequent IgH translocation events associated with B‐cell malignancies. A convincing demonstration of the essential contribution of 3′RR in lymphomagenesis has been provided by transgenic animal models. The mouse 3′RR shares a strong structural homology with the regulatory regions located downstream of each human Cα gene. Mouse models exploring the role of the 3′RR in B‐cell physiology and in malignancies should provide useful indications about the pathophysiology of human cell lymphocyte proliferation.
Changes in Performance of DNA Biosensor Caused by Hydroxyl Radicals
Authors: Nowicka Anna M. , Kowalczyk Agata , Scholz Fritz , Stojek Zbigniew 
Published: November 2010

AbstractA DNA biosensor was exposed to the activity of OH radicals at two selected stages of its preparation/performance. The sensor was immersed in Fenton solution after immobilization of probe ssDNA layer and after the hybridization process with target DNA strands. It appeared that the extent of damage to ssDNA at the sensing layer was much stronger compared to the dsDNA layer. The visualization of the changes in the performance of the sensor caused by the action of OH radicals was done by applying methylene blue as an electrochemical tag. To explain the changes of the voltammetric peak of methylene blue for two selected stages of the sensor preparation the EIS measurements were also performed.
Voltammetric Electronic Tongue in the Analysis of Cava Wines
Authors: Cetó Xavier , Gutiérrez Juan Manuel , Moreno‐Barón Laura , Alegret Salvador , del Valle Manel 
Published: November 2010

AbstractThis work demonstrates the use of a voltammetric electronic tongue formed by five modified graphite‐epoxy electrodes in the qualitative and quantitative analysis of cava wines. The different samples were analyzed using cyclic voltammetry without any sample pretreatment. Recorded data were evaluated by Principal Component Analysis and Discrete Wavelet Transform in order to compress and extract significant features from the voltammetric signals. The preprocessed information was evaluated by an Artificial Neural Network that accomplishes the qualitative classification. Moreover, a preliminary study related to the quantification of sugar amount present was assessed by Second‐Order Standard Addition Method.
Composite PEDOT/Au Nanoparticles Modified Electrodes for Determination of Mercury at Trace Levels by Anodic Stripping Voltammetry
Authors: Giannetto Marco , Mori Giovanni , Terzi Fabio , Zanardi Chiara , Seeber Renato 
Published: November 2010

AbstractElectrode modifications consisting of poly(3,4‐ethylenedioxythiophene) (PEDOT), including gold nanoparticles according to simple though innovative procedure, are developed. The resulting nanocomposite shows interesting performances as electrode material for determination of mercury by anodic stripping technique, even down to concentration levels as low as 0.83 ng/mL. The analytical performance is evaluated by optimized experimental parameters such as the charge spent during the potentiostatic electrodeposition of PEDOT, the preconcentration time, and the waveform parameters for the differential pulse voltammetry (DPV) redissolution scan. No evidence of significant oxidative degradation of the polymer over repeated analysis cycles is evidenced.
Determination of the potassium content of explosive residues using miniaturised isotachophoresis
Authors: Prest Jeff E. , Beardah Matthew S. , Baldock Sara J. , Doyle Sean P. , Fielden Peter R. , Goddard Nicholas J. , Brown Bernard J. Treves 
Published: November 2010

AbstractA new method has been developed to allow the determination of potassium in post‐explosion residues to be made using miniaturised isotachophoresis. The method is based on the use of a caesium leading ion with 4.5 mM 18‐crown‐6 ether added to retard the potassium to allow reliable determinations to be made. With the conditions selected no interference was noted from other small inorganic cations, such as ammonium, barium, calcium, magnesium, sodium or strontium. The method was successfully applied to the analysis of seven samples containing explosive residues obtained from the unconfined burning of several flash powders. The procedure was found to offer good linearity for potassium determinations over the concentration range of 1.25–150 μg/mL with a coefficient of determination of 0.999 achieved.
β‐Amino alcohol selectors for enantioselective separation of amino acids by ligand‐exchange capillary zone electrophoresis in a low molecular weight organogel
Authors: Rizkov Dan , Mizrahi Shaul , Cohen Shmuel , Lev Ovadia 
Published: November 2010

AbstractA new family of copper ligand‐exchange selectors, L‐ or D‐β‐amino alcohols, is employed for the chiral separation of D,L‐dansyl‐amino acids, unmodified amino acid racemates, phenylalanine and tryptophan, and β‐blocker L,D‐propranolol by SDS–micellar electrokinetic chromatography and by electrophoretic chromatography in a low molecular weight organogel (LMOG)‐filled capillary. The LMOG comprised a self‐assembled fibrillar gel of trans‐(1S,2S)‐1,2‐bis‐(dodecylamido) cyclohexane in methanol. The di‐L‐valinol–copper complex exhibited the best performance on LMOG–CE compared with all other β‐amino alcohol–copper selectors. The dependence of chiral resolution on the pH*, the ratio between the copper and the L‐valinol ligand and the concentration of added selector complex in the run buffer were investigated revealing a marked difference between the activity of the copper–valinol and the previously studied copper–valine selector. The optimal separation conditions were achieved using a 2:1 valinol/copper ratio, in accordance with the 2:1 structure of the complex, which was proven by single crystal and powder X‐ray diffractions and by elemental analysis. Unlike the copper–valine selectors that could be used only under acidic conditions (pH* 3.5), the copper–valinol selectors could be used under near‐neutral conditions and even at pH* 9.1. A comparison between SDS–micellar electrokinetic chromatography and LMOG–CE under otherwise identical conditions revealed a significant superior separation on the LMOG‐filled capillaries.
Polyacrylamide grafted on multi‐walled carbon nanotubes for open‐tubular capillary electrochromatography: Comparison with silica hydride and polyacrylate phase matrices
Authors: Chen Jian‐Lian , Hsieh Kai‐Hsin 
Published: November 2010

AbstractA new nanoparticle‐bound polymer stationary phase was prepared by in situ polymerization of methacrylamide (MAA), bis‐acrylamide crosslinker, and carboxylated multi‐walled carbon nanotubes (multi‐walled CNTs; MWNTs), using the abundant double bonds in the cyclopentadienyl rings in MWNT structure, on a silanized capillary. Each intermediate capillary between the synthesis steps was characterized by SEM, by ATR‐IR, and by EOF measurements varying the pH, concentration, and volumetric ratios of ACN in running buffers. The resulting EOF profile was comparable to those of two other capillaries with different phase matrices, silica hydride and polybutyl methacrylate (BMA) phases. With the complex functionality of MWNTs on the hydrophilic polyacrylamide network, the MAA‐CNT capillary was capable of separating diverse samples with a wide range of polarity and dissociation properties using open‐tubular CEC. Besides optimizing CEC conditions, the migration times of samples were analyzed with respect to velocity and retention factors to evaluate electrophoretic and chromatographic contributions to the CEC mechanism. The migration rates of benzoic acids were determined by the electrophoretic mobilities of the various phenolate ions, while phenolic aldehydes and ketones were additionally influenced by chromatographic interactions, such as π–π, electrostatic effects, hydrogen bonding, and hydrophobic interactions. The retention factors were greater for flavonoids, which are polyphenolic, than for simple phenols, but were smaller than those obtained from the hydrophobic BMA‐CNT column. A complete well‐resolved separation of the cationic forms of tetracyclines was acheived either by electrophoresis or by chromatography in the MAA‐CNT capillary, but not in the BMA‐CNT and silica hydride‐CNT capillaries.
White LEDs as broad spectrum light sources for spectrophotometry: Demonstration in the visible spectrum range in a diode‐array spectrophotometric detector
Authors: Piasecki Tomasz , Breadmore Michael C. , Macka Mirek 
Published: November 2010

AbstractAlthough traditional lamps, such as deuterium lamps, are suitable for bench‐top instrumentation, their compatibility with the requirements of modern miniaturized instrumentation is limited. This study investigates the option of utilizing solid‐state light source technology, namely white LEDs, as a broad band spectrum source for spectrophotometry. Several white light LEDs of both RGB and white phosphorus have been characterized in terms of their emission spectra and energy output and a white phosphorus Luxeon LED was then chosen for demonstration as a light source for visible‐spectrum spectrophotometry conducted in CE. The Luxeon LED was fixed onto the base of a dismounted deuterium (D2) lamp so that the light‐emitting spot was geometrically positioned exactly where the light‐emitting spot of the original D2 lamp is placed. In this manner, the detector of a commercial CE instrument equipped with a DAD was not modified in any way. As the detector hardware and electronics remained the same, the change of the deuterium lamp for the Luxeon white LED allowed a direct comparison of their performances. Several anionic dyes as model analytes with absorption maxima between 450 and 600 nm were separated by CE in an electrolyte of 0.01 mol/L sodium tetraborate. The absorbance baseline noise as the key parameter was 5× lower for the white LED lamp, showing clearly superior performance to the deuterium lamp in the available, i.e. visible part of the spectrum.
Promoted negative staining of proteins in SDS‐PAGE using Eosin B compounded with magnesium chloride
Authors: Wang Xu , Hwang Sun‐Young , Cong Wei‐Tao , Jin Li‐Tai , Choi Jung‐Kap 
Published: November 2010

AbstractIn this study, we describe an effective visualizing technique for proteins in SDS‐PAGE based on the organic dye, Eosin B, the sensitivity of which can be further strengthened by the addition of magnesium to the staining solution after electrophoresis. The newly developed protocol is low in cost and easily performed compared with the common methods for protein analysis in 1‐D and 2‐D gels. It provides a much better sensitivity (0.2 ng of single protein band) than that of imidazole–zinc negative stain for fixing and staining within 1 h, and an excellent performance in terms of compatibility with MALDI‐TOF MS. The results show that similar identification scores and numbers of matched peptides were obtained by both methods. Furthermore, the effects of different metal salts on the quality of protein visualization by Eosin B were also investigated. Because of its sensitivity, stability, and safety, this stain may be a more practical method for protein determination in the routine laboratory.