bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute fluid ingestion in the treatment of orthostatic intolerance – important implications for daily practice
Authors: Z’Graggen W. J. , Hess C. W. , Humm A. M. 
Published: April 2010

Background:  Rapid water ingestion improves orthostatic intolerance (OI) in multiple system atrophy (MSA) and postural tachycardia syndrome (PoTS). We compared haemodynamic changes after water and clear soup intake, the latter being a common treatment strategy for OI in daily practice.Methods:  Seven MSA and seven PoTS patients underwent head‐up tilt (HUT) without fluid intake and 30 min after drinking 450 ml of water and clear soup, respectively. All patients suffered from moderate to severe OI because of neurogenic orthostatic hypotension (OH) and excessive orthostatic heart rate (HR) increase, respectively. Beat‐to‐beat cardiovascular indices were measured non‐invasively.Results:  In MSA, HUT had to be terminated prematurely in 2/7 patients after water, but in 6/7 after clear soup. At 3 min of HUT, there was an increase in blood pressure of 15.7(8.2)/8.3(2.3) mmHg after water, but a decrease of 11.6(18.9)/8.1(9.2) mmHg after clear soup (P < 0.05). In PoTS, HUT could always be completed for 10 min, but OI subjectively improved after both water and clear soup. The attenuation of excessive orthostatic HR increase did not differ significantly after water and clear soup drinking.Conclusions:  In MSA, clear soup cannot substitute water for eliciting a pressor effect, but even worsens OI after rapid ingestion. In PoTS, acute water and clear soup intake both result in improvement of OI. These findings cannot solely be explained by difference in osmolarity but may reflect some degree of superimposed postprandial hypotension in widespread autonomic failure in MSA compared to the mild and limited autonomic dysfunction in PoTS.
Surface properties and morphologies of pheohydrane/liquid crystal moisturizer product
Authors: Tsai C. , Lin L.‐H. , Kwan C.‐C. 
Published: April 2010

SynopsisIn this study, we investigated the properties of three different liquid crystal‐type humectants prepared through a simple emulsion process. Under a polarized microscope, we observed liquid crystal textures for each of the three moisturizers. When we applied them onto the hands and face, we found that they all provided effective hydration, with pheohydrane/liquid crystal moisturizer exhibiting superior performance. We also investigated the surface properties (contact angle, zeta potential, surface tension, transmission electron microscopy and scanning electron microscopy analyses) after adding pheohydrane to Formula product.
Off‐line concomitant release of dopamine and glutamate involvement in taste memory consolidation
Authors: Guzmán‐Ramos Kioko , Osorio‐Gómez Daniel , Moreno‐Castilla Perla , Bermúdez‐Rattoni Federico 
Published: April 2010

J. Neurochem. (2010) 114, 226–236.AbstractIt has been postulated that memory consolidation process requires post‐learning molecular changes that will support long‐term experiences. In the present study, we assessed with in vivo microdialysis and capillary electrophoresis whether such changes involve the release of neurotransmitters at post‐acquisition stages. Using conditioned taste aversion paradigm we observed spontaneous off‐line (i.e. in absence of stimulation) dopamine and glutamate reactivation within the insular cortex about 45 min after the stimuli association. These increments did not appear in control groups that were unable to acquire the task, and it seems to be dependent on amygdala activity since its reversible inactivation by tetrodotoxin impaired cortical off‐line release of both neurotransmitters and memory consolidation. In addition, blockade of dopaminergic D1 and/or NMDA receptors before the off‐line activity impaired long‐ but not short‐term memory. These results suggest that off‐line extracellular increments of glutamate and dopamine have a significant functional role in consolidation of taste memory.
Cyclooxygenase‐1 mediates prostaglandin E2 elevation and contextual memory impairment in a model of sustained hippocampal interleukin‐1β expression
Authors: Matousek Sarah B. , Hein Amy M. , Shaftel Solomon S. , Olschowka John A. , Kyrkanides Stephanos , O’Banion M. Kerry 
Published: April 2010

J. Neurochem. (2010) 114, 247–258.AbstractInterleukin (IL)‐1β is a proinflammatory cytokine implicated in several neurodegenerative disorders. Downstream actions of IL‐1β include production of prostaglandin (PG) E2 by increasing expression of cyclooxygenase (COX) enzymes and prostaglandin E synthase (PGES) isoforms. We recently developed a transgenic mouse carrying a dormant human IL‐1β eXcisional Activation Transgene (XAT) for conditional and chronic up‐regulation of IL‐1β in selected brain regions. This model is characterized by regionally specific glial activation, immune cell recruitment, and induction of cytokines and chemokines. Here, we aimed to elucidate the effects of long‐term IL‐1β expression on the PGE2 synthetic pathway and to determine the effects of PGs on inflammation and memory in our model. As expected, PGE2 levels were significantly elevated after IL‐1β up‐regulation. Quantitative real‐time PCR analysis indicated significant induction of mRNAs for COX‐1 and membranous PGES‐1, but not COX‐2 or other PGES isoforms. Immunohistochemistry revealed elevation of COX‐1 but no change in COX‐2 following sustained IL‐1β production. Furthermore, pharmacological inhibition of COX‐1 and use of COX‐1 knockout mice abrogated IL‐1β‐mediated PGE2 increases. Although COX‐1 deficient mice did not present a dramatically altered neuroinflammatory phenotype, they did exhibit improved contextual fear memory. This data suggests a unique role for COX‐1 in mediating chronic neuroinflammatory effects through PGE2 production.
Amphibolite facies retrograde metamorphism of the Zhujiachong eclogite, SE Dabieshan: 40Ar/39Ar age constraints from argon extraction using UV‐laser microprobe, in vacuo crushing and stepwise heating
Authors: QIU H.‐N. , WIJBRANS J. R. , BROUWER F. M. , YUN J.‐B. , ZHAO L.‐H. , XU Y.‐G. 
Published: April 2010

AbstractThe Zhujiachong eclogite in the south‐eastern Dabieshan ultra‐high‐P terrane has been overprinted during retrograde metamorphism, with the development of garnet‐amphibolite mineral assemblages in most rocks in the outcrop. This study is focused on providing age constraints for the retrograde amphibolite facies and greenschist facies mineralogy by 40Ar/39Ar dating. By applying a novel approach of combining three different techniques for extracting argon: laser stepwise heating of single grains and small separates, a spot fusion technique by UV‐laser ablation microprobe on polished sections and an in vacuo crushing technique for liberating radiogenic argon from fluid inclusions, it is demonstrated that an internally consistent thermal history can be derived. The 40Ar/39Ar ages indicate that phengite formed before 265 Ma, probably during the ultra‐high‐P event. Ages associated with amphibolite facies retrograde metamorphism range from 242 to 217 Ma by the analyses of amphibole. Ages of c. 230 Ma were found for the symplectite matrix that formed during retrogression from eclogite pyroxene. Late stage hydrothermal activity leading to the formation of coarse‐grained paragonite and fluid inclusions in vein amphibole was dated at c. 200 Ma. These age results agree well with the mineral crystallization sequence observed from thin‐sections of the retrograded eclogite: phengite → paragonite and amphibole in matrix → amphibole in the corona.
Relationship between adverse effects of antiepileptic drugs, number of coprescribed drugs, and drug load in a large cohort of consecutive patients with drug‐refractory epilepsy
Authors: Canevini Maria Paola , De Sarro Giovambattista , Galimberti Carlo Andrea , Gatti Giuliana , Licchetta Laura , Malerba Ambra , Muscas Giancarlo , La Neve Angela , Striano Pasquale , Perucca Emilio ,   on behalf of the SOPHIE Study Group
Published: April 2010

SummaryPurpose:  To evaluate the adverse effects (AEs) of antiepileptic drugs (AEDs) in adults with refractory epilepsy and their relationship with number of coprescribed AEDs and AED load.Methods:  Patients with refractory epilepsy were enrolled consecutively at 11 tertiary referral centers. AEs were assessed through unstructured interview and the Adverse Event Profile (AEP) questionnaire. AED loads were calculated as the sum of prescribed daily dose (PDD)/defined daily dose (DDD) ratios for each coprescribed AED.Results:  Of 809 patients enrolled, 709 had localization‐related epilepsy and 627 were on polytherapy. AED loads increased with increasing number of AEDs in the treatment regimen, from 1.2 ± 0.5 for patients on monotherapy to 2.5 ± 1, 3.7 ± 1.1, and 4.7 ± 1.1 for those on two, three, and ≥4 AEDs, respectively. The number of spontaneously reported AEs correlated with the number of AEs identified by the AEP (r = 0.27, p < 0.0001). AEP scores did not differ between patients with monotherapy and patients with polytherapy (42.8 ± 11.7 vs. 42.6 ± 11.2), and there was no correlation between AEP scores and AED load (r = −0.05, p = 0.16).Conclusions:  AEs did not differ between monotherapy and polytherapy patients, and did not correlate with AED load, possibly as a result of physicians’ intervention in individualizing treatment regimens. Taking into account the limitations of a cross‐sectional survey, these findings are consistent with the hypothesis that AEs are determined more by individual susceptibility, type of AEDs used, and physicians’ skills, than number of coprescribed AEDs and AED load.
The Use of Stellate Ganglion Block in the Treatment of Panic/Anxiety Symptoms with Combat‐Related Post‐Traumatic Stress Disorder; Preliminary Results of Long‐Term Follow‐Up: A Case Series
Authors: Mulvaney Sean W. , McLean Brian , De Leeuw Jason 
Published: April 2010

AbstractObjective:  Report the successful use of stellate ganglion blocks (SGBs) in two patients experiencing symptoms of post‐traumatic stress disorder (PTSD).Background:  Successful treatment of PTSD with SGB has been reported previously. A similar protocol was employed at Walter Reed Army Medical Center to treat two soldiers with chronic, combat‐related PTSD.Methods:  Both patients received a SGB on the right side at C6 level. The patients' PTSD symptoms were evaluated using the Post‐traumatic Stress Disorder Checklist (PCL). The PCL was administered the day prior to treatment, to establish a baseline, and the day after treatment. The PCL was also utilized during follow‐up visits to quantify the patient's symptomotology. The SGB was administered by an anesthesiologist and the psychometric tests administered by a psychologist.Results:  Both patients experienced immediate, significant and durable relief as measured by the PCL (score minimum 17, maximum 85). In both instances, the pre‐treatment score suggested a PTSD diagnosis whereas the post‐treatment scores did not. One patient requested repeat treatment after 3 months, and the post‐treatment score remained below the PTSD cutoff after 7 additional months of follow‐up. Both patients discontinued all antidepressant and antipsychotic medications while maintaining their improved PCL score.Conclusion:  Selective blockade of the right stellate ganglion at C6 level is a safe and minimally invasive procedure that may provide durable relief from PTSD symptoms, allowing the safe discontinuation of psychiatric medications. ▪
The utility of patient characteristics in predicting severe ADAMTS13 deficiency and response to plasma exchange
Authors: Bentley Melissa J. , Lehman Chris M. , Blaylock Robert C. , Wilson Andrew R. , Rodgers George M. 
Published: April 2010

BACKGROUND: Idiopathic thrombotic thrombocytopenic purpura (TTP) is a rare form of thrombotic microangiopathy (TMA) characterized by extreme deficiency of ADAMTS13, an enzyme responsible for cleavage of von Willebrand factor. Plasma exchange therapy is the cornerstone of current treatment and is ineffective for most other forms of TMA. The availability of ADAMTS13 testing has improved diagnostic accuracy and appropriate selection of patients who are most likely to respond to plasma exchange.STUDY DESIGN AND METHODS: We performed a retrospective chart review of 110 cases of clinically suspected TTP with ADAMTS13 test results from 2005 to the present. The primary goal was to identify presenting clinical and/or laboratory features of patients with ADAMTS13 deficiency that would prove useful in increasing the likelihood of, or excluding the possibility of, TTP. In addition, patient outcomes including alternative diagnoses and response to plasma exchange were reviewed.RESULTS: Significant correlations for severe ADAMTS13 deficiency were seen for four of the observed variables: indirect bilirubin, reticulocyte percentage, creatinine, and platelet count; a fifth variable, D‐dimer, just missed significance but performed well in subsequent analysis. Receiver operator characteristics curves for individual variables had area under the curve (AUC) values ranging from 0.75 to 0.85; a combined model had an AUC of 0.98. In addition, we constructed tree models both for clinical use as a diagnostic algorithm and for recursive partitioning to help establish cutoff points for categorical variables in developing an easy‐to‐use clinical prediction score.CONCLUSION: These results may enable the rapid exclusion and accurate diagnosis of TTP using readily available laboratory data.
The effects of enamel matrix derivative on the proliferation and differentiation of human mesenchymal stem cells
Authors: Jue Seong‐Suk , Lee Won Young , Kwon Yong‐Dae , Kim Young‐Ran , Pae Ahran , Lee Baeksoo 
Published: April 2010

AbstractPurpose: This study was designed to investigate the effect of enamel derivative matrix (EMD) on the proliferation, mineralization, and differentiation of human mesenchymal stem cells (hMSCs).Material and methods: For the proliferation assay, water‐soluble tetrazolium salt‐8 tests were carried out after culturing for 24 and 48 h. For the evaluation of mineralization, Alizarin red S (ARS) tests were performed after 21 days of culturing in an osteogenic medium. In order to investigate some of the bone‐related proteins, namely type I collagen (Col I A2), bone sialoprotein (BSP), and bone γ‐carboxyglutamate (Gla) protein (BGLAP, osteocalcin), real‐time polymerase chain reaction (RT‐PCR) tests were carried out after 2, 3, and 4 weeks of culturing, respectively.Results: The activity of proliferation and mineralization increased significantly depending on the concentration of EMD (P<0.05). In the control group, the expression of Col I A2 decreased, but EMD enhanced its expression over time and was correlated to the concentration. The amount of expression of BSP in this group increased over time, but EMD strikingly suppressed its expression in the fourth week. As well, the amount of expression of BGLAP increased as the culture duration lengthened in the control group. However, the expression of BGLAP was suppressed in the experimental group with EMD.Conclusion: Within the limits of this study, EMD enhanced the proliferation of hMSCs. After evaluation with ARS staining, EMD seemed to enhance mineralization, and the RT‐PCR test revealed that EMD promoted early‐stage osteoblast differentiation by enhancing Col I A2 expression, but exerted an inhibitory effect on the mineralization by lowering the gene expression of BSP and BGLAP. Mineralized nodules formed with EMD may be composed of substances other than normal bone. Because most of the organic matrix of bone is type I collagen, which acts as the mineralization site, bone or bone‐like mineralized mass might have been formed in spite of the different components of the non‐collagenous proteins. To cite this article:
Jue S‐S, Lee WY, Kwon Y‐D, Kim Y‐R, Pae A, Lee B. The effects of enamel matrix derivative on the proliferation and differentiation of human mesenchymal stem cells. Clin. Oral Impl. Res. 21, 2010; 741–746. doi: 10.1111/j.1600‐0501.2009.01901.x
Biofunctionalization of titanium implants with a biomimetic active peptide (P‐15) promotes early osseointegration
Authors: Lutz R. , Srour S. , Nonhoff J. , Weisel T. , Damien C. J. , Schlegel K. A. 
Published: April 2010

AbstractObjectives: The early stages of peri‐implant bone formation play an essential role in the osseointegration and long‐term success of dental implants. By incorporating bioactive coatings, this biofunctionalization of implant surfaces may enhance the attachment of the implant to the surrounding bone and stimulate bone regeneration.Material and methods: To demonstrate faster osseointegration, the surfaces of dental implants were grit‐blasted and acid‐etched. They were then coated with hydroxyapatite (HA) and experimental implants were further coated with a biomimetic active peptide (P‐15) in one of two concentrations. These biofunctionalized samples and controls with no peptide were placed in the forehead region of 12 adult pigs. Six animals were evaluated for a period of 14 or 30 days.Results: Histomorphometric analysis demonstrated that the implants with the high concentration of P‐15 had significantly higher percentage of bone‐to‐implant contact (BIC) at 14 (P=0.018) and 30 (P=0.015) days compared with the other groups. Both concentrations of P‐15 showed increased peri‐implant bone density compared to the control group at 30 days.Conclusion: Biofunctionalization of the implant surface with a biomimetic active peptide leads to significantly increased BIC rates at 14 and 30 days and higher peri‐implant bone density at 30 days. To cite this article:
Lutz R, Srour S, Nonhoff J, Weisel T, Damien CJ, Schlegel KA. Biofunctionalization of titanium implants with a biomimetic active peptide (P‐15) promotes early osseointegration. Clin. Oral Impl. Res. 21, 2010; 726–734. doi: 10.1111/j.1600‐0501.2009.01904.x
Accuracy of measuring the cortical bone thickness adjacent to dental implants using cone beam computed tomography
Authors: Razavi Touraj , Palmer Richard M. , Davies Jonathan , Wilson Ron , Palmer Paul J. 
Published: April 2010

AbstractObjective: To assess the accuracy of measuring the cortical bone thickness adjacent to dental implants using two cone beam computed tomography (CBCT) systems.Material and methods: Ten 4 × 11 mm Astra Tech® implants were placed at varying distances from the cortical bone in two prepared bovine ribs. Both ribs were scanned in a reproducible position using two different CBCT scanners. Ten examiners each carried out four measurements on all 10 implants using the two CBCT systems: vertical distance between the top of the implant and the alveolar crest (IT–AC), and thickness of the cortical bone from the outer surface of the implant threads at 3, 6 and 9 mm from the top of the implant. Ground sections were prepared and bone thickness was measured using a light microscope and a graticule to give a gold standard (GS) measurement.Results: The examiner's measurements were significantly different between CBCT systems for the vertical and thickness dimensions (P<0.001) while measuring the cortical bone thickness between 0.3 and 3.7 mm. Within that range, i‐CAT NG® measurements were consistently underestimated in comparison with the GS. Accuitomo 3D60 FPD® measurements closely approximated the GS, except when cortical bone thickness was <0.8 mm. The mean percentage errors from the GS at 3, 6 and 9 mm measurement levels were 68%, 28% and 18%, respectively, for i‐CAT NG® and 23%, 5% and 6%, respectively, for Accuitomo 3D60 FPD®.Conclusions: Within the limitations of this study, it was concluded that i‐CAT NG® (voxel size 0.3) may not produce sufficient resolution of the thin cortical bone adjacent to dental implants and, therefore, the measurements may not be accurate; whereas, Accuitomo 3D60 FPD® (voxel size 0.125) may produce better resolution and more accurate measurement of the thin bone. To cite this article: Razavi T, Palmer RM, Davies J, Wilson R, Palmer PJ. Accuracy of measuring the cortical bone thickness adjacent to dental implants using cone beam computed tomography. Clin. Oral Impl. Res. 21, 2010; 718–725. doi: 10.1111/j.1600‐0501.2009.01905.x
Effect of six different peri‐implantitis disinfection methods on in vivo human oral biofilm
Authors: Gosau Martin , Hahnel Sebastian , Schwarz Frank , Gerlach Till , Reichert Torsten E. , Bürgers Ralf 
Published: April 2010

AbstractObjective: The aim of this human in vivo pilot study was to evaluate the efficacy of six antimicrobial agents on the surface decontamination of an oral biofilm attached to titanium implants.Design: For in vivo biofilm formation, we fixed titanium specimens to individual removable acrylic upper jaw splints (14 specimens in every splint), which were worn by four volunteers overnight for 12 h. The specimens were then treated with different antimicrobial agents for 1 min (Sodium hypochlorite, Hydrogen peroxide 3%, Chlorhexidingluconate 0.2%, Plax, Listerine, citric acid 40%). Afterwards, we quantified the total bacterial load and the viability of adhering bacteria by live or dead cell labelling in combination with fluorescence microscopy.Results: The total bacterial load on the titanium surfaces was significantly higher after incubation in the control solution phosphate‐buffered saline (PBS) than after disinfection in sodium hypochlorite, hydrogen peroxide, chlorhexidine, Plax, Listerine, and citric acid. Furthermore, a significantly lower ratio between dead and total adhering bacteria (bactericidal effect) was found after incubation in control PBS, Plax mouth rinse, and citric acid than after incubation in sodium hypochlorite, hydrogen peroxide, chlorhexidine, and Listerine.Conclusions: All tested antiseptics seem to be able to reduce the total amount of microorganisms accumulating on titanium surfaces. Furthermore, sodium hypochlorite, hydrogen peroxide, chlorhexidine, and Listerine showed a significant bactericidal effect against adhering bacteria. To cite this article:
Gosau M, Hahnel S, Schwarz F, Gerlach T, Reichert TE, Bürgers R. Effect of six different peri‐implantitis disinfection methods on in vivo human oral biofilm. Clin. Oral Impl. Res. 21, 2010; 866–872. doi: 10.1111/j.1600‐0501.2009.01908.x