bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrating geo‐referenced multiscale and multidisciplinary data for the management of biodiversity in livestock genetic resources
Authors: Joost S. , Colli L. , Baret P. V. , Garcia J. F. , Boettcher P. J. , Tixier‐Boichard M. , Ajmone‐Marsan P. ,   The GLOBALDIV Consortium
Published: April 2010

SummaryIn livestock genetic resource conservation, decision making about conservation priorities is based on the simultaneous analysis of several different criteria that may contribute to long‐term sustainable breeding conditions, such as genetic and demographic characteristics, environmental conditions, and role of the breed in the local or regional economy. Here we address methods to integrate different data sets and highlight problems related to interdisciplinary comparisons. Data integration is based on the use of geographic coordinates and Geographic Information Systems (GIS). In addition to technical problems related to projection systems, GIS have to face the challenging issue of the non homogeneous scale of their data sets. We give examples of the successful use of GIS for data integration and examine the risk of obtaining biased results when integrating datasets that have been captured at different scales.
Genetic diversity in farm animals – a review
Authors: Groeneveld L. F. , Lenstra J. A. , Eding H. , Toro M. A. , Scherf B. , Pilling D. , Negrini R. , Finlay E. K. , Jianlin H. , Groeneveld E. , Weigend S. ,   The GLOBALDIV Consortium
Published: April 2010

SummaryDomestication of livestock species and a long history of migrations, selection and adaptation have created an enormous variety of breeds. Conservation of these genetic resources relies on demographic characterization, recording of production environments and effective data management. In addition, molecular genetic studies allow a comparison of genetic diversity within and across breeds and a reconstruction of the history of breeds and ancestral populations. This has been summarized for cattle, yak, water buffalo, sheep, goats, camelids, pigs, horses, and chickens. Further progress is expected to benefit from advances in molecular technology.
Clinical predictors of severe malarial anaemia in a holoendemic Plasmodium falciparum transmission area
Authors: Novelli Enrico M. , Hittner James B. , Davenport Gregory C. , Ouma Collins , Were Tom , Obaro Stephen , Kaplan Sandra , Ong’echa John M. , Perkins Douglas J. 
Published: April 2010

SummarySevere malarial anaemia (SMA) is a common complication of Plasmodium falciparum infections, resulting in mortality rates that may exceed 30% in paediatric populations residing in holoendemic transmission areas. One strategy for reducing the morbidity and mortality associated with SMA is to identify clinical predictors that can be readily recognized by caregivers for prompt therapeutic interventions. To determine clinical predictors of SMA, Kenyan children (3–36 months, n = 671) presenting with acute illness at a rural hospital in Siaya District were recruited. Demographic, clinical, laboratory and haematological parameters were measured upon enrolment. As human immunodeficiency virus‐1 and bacteraemia promote reduced haemoglobin (Hb) concentrations, children with these infections were excluded from the analyses. Children with P. falciparum mono‐infections (n = 355) were stratified into three groups: uncomplicated malaria (Hb ≥ 110 g/l); non‐SMA (60 ≤ Hb < 109), and SMA (Hb < 60 g/l). SMA was characterized by a younger age, monocytosis, thrombocytopenia, reticulocytosis, reduced erythropoiesis, elevated pigment‐containing monocytes (PCM), respiratory distress, conjunctival and palmar pallor, splenomegaly, signs of malnutrition, and protracted fever and emesis. Logistic regression analysis demonstrated that age, reticulocyte count, presence of PCM and conjunctival and palmar pallor were significant predictors of SMA. Recognition of these clinical signs in children residing in resource‐poor settings may help to guide the identification and management of SMA.
Diagnosis of post‐transplant lymphoproliferative disorder in solid organ transplant recipients – BCSH and BTS Guidelines
Authors: Parker Anne , Bowles Kristin , Bradley J. Andrew , Emery Vincent , Featherstone Carrie , Gupte Girish , Marcus Robert , Parameshwar Jayan , Ramsay Alan , Newstead Charles ,   (British Transplantation Society Lead) Writing group: On behalf of the Haemato‐oncology Task Force of the British Committee for Standards in Haematology and British Transplantation Society
Published: April 2010

SummaryA joint working group established by the Haemato‐oncology subgroup of the British Committee for Standards in Haematology (BCSH) and the British Transplantation Society (BTS) has reviewed the available literature and made recommendations for the diagnosis and management of post‐transplant lymphoproliferative disorder (PTLD) in adult recipients of solid organ transplants. This review details the risk factors predisposing to development, initial features and diagnosis. It is important that the risk of developing PTLD is considered when using post transplant immunosuppression and that the appropriate investigations are carried out when there are suspicions of the diagnosis. These must include tissue for histology and computed tomography scan to assess the extent of disease. These recommendations have been made primarily for adult patients, there have been some comments made with regard to paediatric practice.
Pharmacokinetics and pharmacokinetic/pharmacodynamic associations of ofatumumab, a human monoclonal CD20 antibody, in patients with relapsed or refractory chronic lymphocytic leukaemia: a phase 1–2 study
Authors: Coiffier Bertrand , Losic Nedjad , Rønn Birgitte Biilmann , Lepretre Stéphane , Pedersen Lars Møller , Gadeberg Ole , Frederiksen Henrik , Van Oers Marinus H. J. , Wooldridge James , Kloczko Janusz , Holowiecki Jerzy , Hellmann Andrzej , Walewski Jan , Robak Tadeusz , Petersen Jørgen 
Published: April 2010

SummaryThe purpose of this phase 1–2 study was to investigate the association between the pharmacokinetic properties of ofatumumab, a human monoclonal CD20 antibody, and outcomes in 33 patients with relapsed/refractory chronic lymphocytic leukaemia receiving 4 weekly infusions of ofatumumab. The ofatumumab concentration profiles were fitted well by a two‐compartment model with different elimination rate constant at first infusion compared to the remaining infusions in line with the observed rapid and sustained B‐cell depletion. Exposure to ofatumumab was linked to clinical outcomes: high exposure was associated with higher probability of overall clinical response and longer progression‐free survival. This association still remained statistically significant even when adjusting for relevant baseline covariates including tumour burden. The trial was registered at http://www.clinicaltrials.gov (NCT00093314).
NT‐proBNP as a marker of cardiopulmonary status in sickle cell anaemia in Africa
Authors: Aliyu Zakari Y. , Suleiman Aisha , Attah Ester , Mamman Aisha I. , Babadoko Aliyu , Nouraie Mehdi , Mendelsohn Laurel , Kato Gregory J. , Gordeuk Victor R. , Gladwin Mark T. 
Published: April 2010

SummaryN‐terminal (NT) pro‐brain natriuretic peptide (proBNP) ≥160 ng/l has a 78% positive predictive value for pulmonary hypertension and is associated with increased mortality in US sickle cell disease patients, but the importance in sickle cell disease patients in Africa is not known. In a cross‐sectional study at Ahmadu Bello University Teaching Hospital, Shika‐Zaria, Nigeria, we studied 133 hydroxycarbamide‐naïve Nigerian sickle cell anaemia patients aged 18–52 years at steady‐state and 65 healthy controls. Twenty‐six percent of patients versus 5% of controls had NT‐proBNP ≥160 ng/l (P = 0·0006). By logistic regression among the patients, human immunodeficiency virus seropositivity, higher serum ferritin and lower haemoglobin or higher lactate dehydrogenase independently predicted elevated NT‐proBNP. After adjustment for haemoglobin concentration, elevated NT‐proBNP concentration was associated with an estimated 7·8‐fold increase in the odds of severe functional impairment, defined as an inability to walk more than 300 m in 6 min (95% confidence interval 1·5–32·6; P = 0·005). Similarly, elevated tricuspid regurgitation velocity was associated with an estimated 5·6‐fold increase in the odds of functional impairment (95% confidence interval 1·5–21·0; P = 0·011). In conclusion, NT‐proBNP elevation is common and is associated with markers of anaemia, inflammation and iron status and with severe functional impairment among sickle cell anaemia patients in Nigeria.
A meta‐synthesis describing the relationships between patients, informal caregivers and health professionals in home‐care settings
Authors: Lindahl Berit , Lidén Eva , Lindblad Britt‐Marie 
Published: April 2010

Aim. The present study describes, through a meta‐synthesis, the relationship between patients, informal caregivers and health professionals involved in home care.Background. Today, many people receive help from health care professionals in their homes with the consequence that, for many health care professionals, their working place is the patients’ homes. Research that addresses the dynamics in the caring relationship in home care seems to be rare.Design. A meta‐synthesis is an integrated interpretation of qualitative research findings, which is more substantive than the results from each individual investigation.Method. We performed a systematic literature search regarding studies published during the period 1992–2005, using the search terms home nursing, professional and home health care. The included studies described relations in a home care context, between health professionals and patients or relations between health professionals, patients and their informal caregivers published in the same study.Results. The findings showed that when professionals entered people’s home, the private area changed. The study presents an interpretation of the changed meanings of home as the place and space for professional care. We described the meanings of the relationship in two main themes with subthemes. The main themes are ‘being there’ and ‘home care as a co‐creation’. The understanding of relationships in home care is seen as the development of a professional friendship. This concept is reflected on through the writings of Aristotle and Alberoni.Conclusion. To address these concerns, it is important that home care providers, recipients and their family members develop friendships. These friendships should be a part of any professional relationship.Relevance to clinical practice. When health professionals enter patients’ homes, they have to be aware of the risk of transgressing borders of privacy. In addition, devaluing patients’ or their informal caregivers’ knowledge and their opinions about the care is interpreted as an exercise of institutional power.
On the relationship between motor performance and executive functioning in children with intellectual disabilities
Authors: Hartman E. , Houwen S. , Scherder E. , Visscher C. 
Published: April 2010

AbstractBackground  It has been suggested that children with intellectual disabilities (ID) have motor problems and higher‐order cognitive deficits. The aim of this study was to examine the motor skills and executive functions in school‐age children with borderline and mild ID. The second aim was to investigate the relationship between the two performance domains.Methods  Sixty‐one children aged between 7 and 12 years diagnosed with borderline ID (33 boys and 28 girls; 71 < IQ < 79) and 36 age peers with mild ID (24 boys and 12 girls; 54 < IQ < 70) were assessed. Their abilities were compared with those of 97 age‐ and gender‐matched typically developing children. Qualitative motor skills, i.e. locomotor ability and object control, were evaluated with the Test of Gross Motor Development (TGMD‐2). Executive functioning (EF), in terms of planning ability, strategic decision‐making and problem solving, was gauged with the Tower of London (TOL) task.Results  Compared with the reference group, the full ID cohort scored significantly lower on all assessments. For the locomotor skills, the children with mild ID scored significantly lower than the children with borderline ID, but for the object control skills and the TOL score, no significant differences between the two groups were found. Motor performance and EF correlated positively. At the most complex level, the TOL showed decision time to be a mediator between motor performance and EF: the children with the lower motor scores had significantly shorter decision times and lower EF scores. Analogously, the children with the lower object control scores had longer execution times and lower EF scores.Conclusions  The current results support the notion that besides being impaired in qualitative motor skills intellectually challenged children are also impaired in higher‐order executive functions. The deficits in the two domains are interrelated, so early interventions boosting their motor and cognitive development are recommended.
The specificity of allelopathy in rice (Oryza sativa)
Authors: SEAL A N , PRATLEY J E 
Published: April 2010

Seal AN & Pratley JE (2010). The specificity of allelopathy in rice (Oryza sativa). Weed Research50, 303–311.SummaryThe objective of the current research was to evaluate the capability of individual rice cultivars to have multi‐weed suppressive effects against five currently and potentially important Australian aquatic rice weeds. These weed species are problematic, as resistance occurs to the major herbicide available for their control. Twenty‐seven rice cultivars were screened in the laboratory for their allelopathic impact using the Equal Compartment Agar Method against the weeds. Significant differences existed between rice cultivars in their ability to suppress the root growth of each weed studied. Correlations of root inhibition values between combinations of the tested weeds were undertaken to establish rice cultivar inhibition against multiple weed species. All correlations were significant. The highest correlation occurred with Alisma lanceolatum and Sagittaria graminea (r = 0.93), closely related species, and the lowest (r = 0.58) was obtained when comparing Echinochloa crus‐galli with S. montevidensis, being unrelated species. The seven most allelopathic cultivars overall inhibited mean weed growth by more than 90%, demonstrating that particular rice cultivars can significantly inhibit multiple weed species. Cluster analysis of the available pedigree information for the tested rice cultivars showed that several rice cultivars with high allelopathic impact in bioassay were grouped in two clusters. This information is useful for plant breeders aiming to incorporate weed allelopathic capability into new crop cultivars.
Non‐invasive ICG‐clearance: a useful tool for the management of hepatic artery thrombosis following liver transplantation
Authors: Levesque E. , Hoti E. , Azoulay D. , Adam R. , Samuel D. , Castaing D. , Saliba F. 
Published: April 2010

Levesque E, Hoti E, Azoulay D, Adam R, Samuel D, Castaing D, Saliba F. Non‐invasive ICG‐clearance: a useful tool for the management of hepatic artery thrombosis following liver transplantation.  Clin Transplant 2011: 25: 297–301. © 2010 John Wiley & Sons A/S.Abstract:  Background:  The clinical presentation of hepatic artery thrombosis (HAT) post‐liver transplantation (LT) varies considerably. Doppler ultrasonography (Doppler US) is the first line investigation, with a diagnostic sensitivity for HAT as high as 92%. Because indocyanine green (ICG) elimination from the blood depends among other factors on the hepatic blood flow, we hypothesized that plasma disappearance rate of indocyanine green (PDR‐ICG) can be influenced by the flow in the hepatic artery. Thus, we evaluated the role of PDR‐ICG measurement in HAT diagnosis in post‐LT patients.Patients and methods:  Fourteen liver transplant patients with no visible flow in the hepatic artery (Doppler US) were identified. Of the 14, seven patients had HAT confirmed by CT‐angiography. The PDR‐ICG measurement, an investigation routinely used in our center, was performed in all 14 patients.Results:  The PDR‐ICG in patients with HAT was significantly lower than in patients without HAT (5.8 ± 4.3 vs. 23.8 ± 7.4%/min, p = 0.0009). In patients with HAT, after the revascularization, the PDR‐ICG value increased (5.8 ± 4.3 vs. 15.6 ± 3.5%/min, p = 0.006).Conclusion:  The ICG elimination may be an adjunct diagnostic tool in the management of patients with suspected HAT following LT.
Neuroprotective effect of asymmetric dimethylarginine against 1‐methyl‐4‐phenylpyridinium ion‐induced damage in PC12 cells
Authors: Tang Xiao‐Qing , Li Yu‐Juan , Zhao Jing , Shen Xing‐Tian , Yang Chun‐Tao , Fan Li‐Li , Hu Bi , Li Yuan‐Jian , Liao Duan‐Fan 
Published: April 2010

Summary1. Asymmetric dimethylarginine (ADMA) is a well‐known endogenous nitric oxide synthase (NOS) inhibitor. Although it has been shown to be a novel risk marker in cardiovascular medicine and chronic kidney disease, we speculated that in some states associated with excess of nitric oxide (NO), such as 1‐methyl‐4‐phenylpyridinium ion (MPP+)‐induced neuronal injury, ADMA might be protective by limiting the toxic effect of high concentrations of NO.2. The aim of the present study is to explore the protection of ADMA against MPP+‐induced apoptosis and the molecular mechanisms underlying in PC12 cells.3. We found that exogenous application of ADMA obviously protected PC12 cells against MPP+‐induced cytotoxicity and apoptosis not only by reducing the loss of mitochondrial membrane potential, but also by attenuating an increase in intracellular reactive oxygen species. Moreover, ADMA attenuated MPP+‐induced excessive activation of nitric oxide synthase and overproduction of NO.4. The results of the present study suggest that the protection caused by ADMA is related to preserving mitochondrial membrane potential and attenuating the MPP+‐induced intracellular reactive oxygen species generation through inhibiting nitric oxide synthase activity and limiting NO generation.
RNA interference‐mediated silencing of UBCH10 gene inhibits colorectal cancer cell growth in vitro and in vivo
Authors: Chen Shi‐Min , Jiang Chun‐Ying , Wu Jian‐Ying , Liu Bin , Chen Ying‐Jian , Hu Cheng‐Jin , Liu Xian‐Xi 
Published: April 2010

Summary1. UbcH10 is the cancer‐related E2 ubiquitin‐conjugating enzyme, and its overexpression has been demonstrated in a variety of malignancies. The aim of the present study is to silence UbcH10 gene by RNA interference (RNAi) and to observe its inhibitory effect on the colorectal cancer cell growth in vitro and in vivo.2. We constructed the expression vector pGPU6/GFP/Neo/UbcH10‐RNAi (pUbcH10‐RNAi), which contained a UbcH10 short hairpin RNA expression cassette. Then the UbcH10 gene silencing cell lines LoVo/UbcH10‐RNAi and HT‐29/UbcH10‐RNAi were established. Reverse transcription‐polymerase chain reaction and western blot analysis were used to evaluate the expression of the UbcH10 gene. Cell Counting Kit‐8 was used to assess properties of tumour cell growth in vitro. Flow cytometry was used to detect the effect of pUbcH10‐RNAi on the cell cycle of colorectal cancer cells. Furthermore, the anti‐tumour effects of pUbcH10‐RNAi were evaluated in vivo in a nude mouse xenografts model.3. Results demonstrated that UbcH10 gene expression was significantly decreased in pUbcH10‐RNAi treated cells. Colorectal cancer cells growth was markedly suppressed in the pUbcH10‐RNAi group compared with control conditions and colorectal cancer cells were arrested in the G2‐M phase. In vivo, the downregulation of UbcH10 gene expression by pUbcH10‐RNAi also inhibited tumour growth in a nude mice xenograft model.4. Our study suggests that RNA interference‐mediated silencing of UbcH10 gene has anti‐tumour activity on colorectal cancer and might have therapeutic potential for the treatment of colorectal cancer.
Episodic vomiting and headache in children: consider occipital epilepsy
Authors: Lope Raja Juanita Raja , Wong Sau Wei , Wu Loo Ling 
Published: April 2010

AbstractChildren with occipital seizures often have ictal autonomic symptoms such as pallor and vomiting and lack motor manifestations. This has lead to misdiagnosis of occipital seizures in children. The following case report highlights the clinical features of a child with occipital epilepsy misdiagnosed as having migraine. The aetiology of symptomatic occipital epilepsy will be discussed.
Hypokalaemia and failure to thrive: report of a misleading onset
Authors: Conti Giovanni , Vitale Agata , Tedeschi Silvana , Syrén Marie‐Louise , Pantano Roberta , Chimenz Roberto , Fede Salvatore , La Torre Francesco , Coviello Domenico , Fede Carmelo 
Published: April 2010

Aim:  We report a case of Gitelman Syndrome (GS) in a 9‐year‐old girl, previously diagnosed as a Bartter syndrome at one year of life.Methods:  She had been treated with potassium, for over 8 years and was admitted because of fatigue, numbness and weakness of both legs. The patient has typical laboratory findings, including hypokalemia, metabolic alkalosis, hypomagnesemia, and hypocalciuria, thus GS was suspected.Results:  Genetic analysis was performed two mutations IVS9(+1)G>T were detected in the thiazide‐sensitive Na‐Cl cotransporter (TSC) gene (SLC12A3), thus she was diagnosed as having GS. She was treated with oral potassium and magnesium supplements with resolution of the symptoms.Conclusion:  This case reminded us that doctors should be alert to the initial presentation of renal tubular diseases. Detailed electrolyte analysis, hormone evaluations and clinic follow‐up are mandatory for their correct differential diagnosis.
Transgenerational tobacco smoke exposure and childhood cancer: An observational study
Authors: Ortega‐García Juan A , Martin Marlene , López‐Fernández María T , Fuster‐Soler Jose L , Donat‐Colomer Joaquín , López‐Ibor Blanca , Claudio Luz , Ferrís‐Tortajada Josep 
Published: April 2010

Aim:  Although tobacco smoke is an established risk factor for adult cancer, studies of the association between parental smoking and childhood cancer have produced inconsistent results. To investigate the transgenerational relationship between pre‐natal and post‐natal tobacco smoke exposure from the grandmother's pregnancies until after the post‐natal period and childhood cancer.Methods:  Exposure to tobacco smoke was recorded for three generations. Data were collected through personal interviews using the paediatric environmental history, and were compared among 128 children with cancer and 128 matched controls. The contingency tables and a logistic multivariable regression model were used to control for possible confounding factors.Results:  Smoke exposure during oogenesis (maternal grandmother smokers) – odds ratio (OR) 2.2 (95% confidence interval (CI) 1.1–4.9) – and during the mother’ pregnancies – OR 1.8 (95% CI 1.1–3.3) – were significantly associated with an increased risk of childhood cancer.Conclusions:  Tobacco smoke exposure during the grandmother's and mother's pregnancies increase the risk of cancer in the descendants. The results suggest that the biological plausibility of the association between parental smoking and paediatric cancer can be explained by the large latency period of paediatric carcinogenesis.