bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Analytical Approach by Tri-Combination of Gradient UFLC, Response Surface Methodology and Green Chemistry Principle for Simultaneous Quantification of Azelnidipine and Chlorthalidone in Rabbit Plasma
Authors: Bhagyalakshmi C , Rekha T N , Longkumer Piyongsola , Dutta Koushik Nandan , Sahariah Bhargab Jyoti , Ramesh B , Majumder Manish 
Published: May 2024

Abstract
In this study, a sustainable and eco-friendly method is developed to quantify azelnidipine and chlorthalidone in rabbit plasma by gradient liquid chromatography based on green chemistry principle and analytical quality by design. The separation was achieved on a Shim pack C18 (25 cm × 5 cm × 4.6 μm) column with L1 packing. The mobile phase compromised of ethanol and 50-Mm ammonium acetate buffer (pH.6) at flow rate of 0.6 mL/min with 25-min runtime. The resolution and asymmetric factor were identified as critical analytical attributes (CAAs). The screening studies employing Control Noise Experimentation revealed that mobile phase pH, flow rate and ethanol concentration at 6 and 15 min significantly affected the CAAs method. The critical method parameters were optimized using Central Composition design. Chromatogram showed peak of the drugs at retention time of 9.03 min for chlorthalidone and 16.83 min for azelnidipine. The greenness score of the analytical method was found to be 1876.43 using analytical method greenness score calculator. The validation of the developed method was done which showed linearity at the range of 16–520 ng/mL, with R2 of 0.9992 and 0.9996 for azelnidipine and chlorthalidone, respectively, furthermore accuracy, precision, recovery and stability studies are carried out.
Optimization of Extraction of Four Components from Radix Scrophulariae with Natural Deep Eutectic Solvents and Evaluation of Extract’s Antioxidant Activity
Authors: Huang Ping , Zhou Yanxia , Ruan Fei , Sun Jianyu , Shen Jinglin , Chen Hongmei 
Published: May 2024

Abstract
In this research, eight natural deep eutectic solvents (NaDESs) consisting of food-grade ingredients were screened for the extraction of four bioactive compounds (acteoside, cinnamic acid, angoroside C and harpagoside) from radix scrophulariae (RS). Among these NaDESs, Proline-Glycerol NaDES with higher comprehensive score was selected. The Criteria Importance Through Intercriteria Correlation (CRITIC) was applied to calculate the information entropy and the weight of indexes, and figured out a comprehensive score. The weights of acteoside, cinnamic acid, angoroside C and harpagoside were 0.369, 0.172, 0.241 and 0.218, respectively. Response surface methodology (RSM) mathematical model was used to optimize the extraction parameters. The optimal extraction parameters were as follows: extraction time with 42.21 min, NaDES concentration with 52.89%, solid-to-liquid ratio with 1 : 37.05 g/mL and the predicted value of comprehensive score was 0.885. Under the optimal condition, the comprehensive score was 0.903 ± 0.005. Finally, the antioxidant activity experiment revealed that the 1,1-Diphenyl-2-picrylhydrazyl · radical scavenging activity and hydroxyl radical scavenging activity of the extract at 2.0 mg/mL and 1.5 mg/mL were approximately equal to those of ascorbic acid, respectively. The results showed that the extraction condition optimized by RSM combined with CRITIC was reasonable and dependable, and the extract of radix scrophulariae exhibited good antioxidant activity.
Streamlining atrial fibrillation ablation management using a digitization solution
Authors: O’Brien Jim , Valsecchi Sergio , Seaver Fionnuala , Rosalejos Lorena , Arellano Diana , Laurilla Kristine , Jauvert Gael , Fitzpatrick Noel , Tahin Tamas , Keelan Ted , Galvin Joseph , Szeplaki Gabor 
Published: May 2024

Abstract

Aims
Catheter ablation is a widely accepted intervention for atrial fibrillation (AF) management. Prior to undertaking this procedure, thorough patient education on its efficacy and potential complications is crucial. Additionally, educating patients about stroke risk management and anticoagulant therapy is imperative. At Mater Private Hospital in Dublin, we implemented a solution, integrating a customized treatment pathway and a mobile application. This patient-centred approach aims to optimize the clinical management of AF catheter ablation candidates, focusing on knowledge gaps and adherence to guideline-based care to enhance overall outcomes.

Methods and results
The application automates pre-operative assessments and post-operative support, facilitating seamless patient–clinician communication. During the observation period (September 2022–April 2023), 63 patients installed the app. Patient adherence to the pathway was strong, with 98% of patients actively engaging in the treatment pathway and with 81% completing all pre-operative tasks. The average enrolment-to-admission duration was 14 days, and post-ablation tasks were fulfilled by 62% of patients within an average of 36 days. Operators perceived the solution as user-friendly and effective in enhancing patient connectivity. Patient satisfaction was high, and knowledge about AF improved notably through the solution, particularly concerning the recognition of symptoms and anticoagulation therapy-related complications.

Conclusion
Our findings demonstrate the successful implementation of the app-based Ablation Solution, showcasing widespread patient use, improved adherence, and enhanced understanding of AF and its treatments. The system effectively connects healthcare providers with patients, offering a promising approach to streamline AF catheter ablation management and improve patient outcomes.
Cardiovascular risk factors and aortic valve stenosis: towards 10-year absolute risk charts for primary prevention
Authors: Kjeldsen Emilie W , Thomassen Jesper Q , Rasmussen Katrine L , Nordestgaard Børge G , Tybjærg-Hansen Anne , Frikke-Schmidt Ruth 
Published: May 2024

Abstract

Aims
Due to ageing populations, the incidence of aortic valve stenosis (AVS) is increasing steeply. Since no medical therapy is available but only surgical interventions, it is highly warranted to identify modifiable risk factors for early prevention. The aim of the study was to investigate the associations of cardiovascular risk factors with AVS and to create 10-year absolute risk scores for use in primary prevention.

Methods and results
In the Copenhagen General Population Study (n = 93 979), lifestyle data, biochemical measures, and confounders were assessed at baseline. Risk factors with the strongest association with AVS from Cox regression analyses were included in 10-year risk prediction models. Ten-year absolute risk scores were conducted using the method of Fine–Gray proportional sub-hazards models, accounting for competing events. One thousand one hundred and thirty-two individuals developed AVS during follow-up. Of well-known cardiovascular risk factors, those that associated with AVS included increasing levels of remnant cholesterol, triglycerides, lipoprotein(a) [Lp(a)], systolic blood pressure, and body mass index, low adherence to Danish dietary guidelines, current smoking, high alcohol consumption, lipid-lowering therapy, and diabetes mellitus. Ten-year absolute risk scores increased when compiling the most important risk factors for AVS: age, sex, body mass index, systolic blood pressure, Lp(a), and diabetes. Ten-year absolute risk increased from &lt;1 to 19%.

Conclusion
The presence of cardiovascular risk factors is associated with AVS, supporting that this disease, at least partly, may be modifiable through lifestyle changes. Risk charts combining cardiovascular risk factors have the potential to identify high-risk individuals, offering opportunities for preventive strategies.
Comparing healthcare needs by language: interpreted Arabic and Somali telehealth calls in two regions of Sweden, 2014–18
Authors: Martin Leah J , Kühlmann-Berenzon Sharon , Azerkan Fatima , Bjelkmar Pär 
Published: May 2024

Abstract

Background
Limited language fluency can impede healthcare system navigation. In Sweden, the national telehealth line (Healthcare Guide 1177) offers interpretation in Arabic and Somali. We compared calls by language to identify differences in healthcare use for immigrant populations, focusing on three contact causes: pregnancy; vomiting or nausea in children; and worry/anxiety.

Methods
We conducted a cross-sectional analysis of 3.9 million (n = 18 351 Arabic, n = 7199 Somali) telehealth calls (2014–18). Using multivariable logistic regression, we investigated associations between language of the call (Arabic, Somali, non-interpreted) and each contact cause. Potential confounders (age, region, year, and additionally for vomiting or nausea, month) and an interaction between age and language were considered.

Results
Compared with non-interpreted calls, interpreted calls were associated with increased odds of being for pregnancy, especially for 19 to 29-year-olds [adjusted odds ratio (aOR) (95% CI) = 4.04 (3.66–4.46) and 4.60 (4.05–5.23), for Arabic and Somali calls, respectively]. Vomiting or nausea showed similar results, with aOR increasing with age: from 0.90 (0.75–1.07) (Arabic, &lt;1 year) to 3.79 (2.86–5.01) (Somali, 5–9 years). In contrast, in unadjusted analyses, Arabic and Somali calls were associated with decreased odds of being for worry/anxiety [OR = 0.47 (0.38–0.58) and 0.34 (0.21–0.50)], respectively, compared with non-interpreted calls.

Conclusion
Our results suggest callers to the interpreted lines may need additional assistance navigating the healthcare system for pregnancy and for vomiting or nausea among children. These findings can inform healthcare services planning for immigrants to Sweden and highlight a novel use of telehealth data as a way to uncover disparities in healthcare use within a multi-linguistic population.
Trends in oesophageal cancer mortality in Montenegro, 1990–2018
Authors: Nedović Vuković Mirjana , Jakšić Marina , Smolović Brigita , Lukić Miloš , Bukumirić Zoran 
Published: May 2024

Abstract

Background
Oesophageal cancer (OC) is a significant public health issue, despite the decreasing trends in OC mortality rates observed globally in the past decades. The objective of our study is to analyze the pattern of OC mortality in Montenegro between 1990 and 2018 and contribute to the development of a national long-term strategy for the prevention and control of this malignancy.

Methods
The data on OC death cases in Montenegro between 1990 and 2018 were collected. The mortality rates were standardized according to the World Standard Population. The Joinpoint, Linear and Poisson regressions were applied to analyze the OC mortality trend.

Results
Joinpoint regression analysis showed an increase in death rates for men and the overall level which were not statistically significant. However, the number of cases increases significantly with an average annual percentage change (AAPC) increase of 2.6% for the overall level [AAPC (95% CI)=2.6 (1.0–4.2); P = 0.002] at the expense of the increase in men, which on average was 2.6% annually [AAPC (95%CI) = 2.6 (1.2–4.1); P = 0.001]. The age groups 55–64 and 65–74 have the highest percentage of deaths cases from OC with 30.6% and 31.4%, respectively.

Conclusion
Montenegro has witnessed a recent increase in the number of deaths from OC, although the mortality rates remain stable. National strategies to further reduce mortality rates for OC are necessary. Individuals aged 55–64 and 65–74 need specific attention during the ongoing monitoring of this cancer.
Field-based high-throughput phenotyping enhances phenomic and genomic predictions for grain yield and plant height across years in maize
Authors: Adak Alper , DeSalvio Aaron J , Arik Mustafa A , Murray Seth C 
Published: May 2024

Abstract
Field-based phenomic prediction employs novel features, like vegetation indices (VIs) from drone images, to predict key agronomic traits in maize, despite challenges in matching biomarker measurement time points across years or environments. This study utilized functional principal component analysis (FPCA) to summarize the variation of temporal VIs, uniquely allowing the integration of this data into phenomic prediction models tested across multiple years (2018–2021) and environments. The models, which included 1 genomic, 2 phenomic, 2 multikernel, and 1 multitrait type, were evaluated in 4 prediction scenarios (CV2, CV1, CV0, and CV00), relevant for plant breeding programs, assessing both tested and untested genotypes in observed and unobserved environments. Two hybrid populations (415 and 220 hybrids) demonstrated the visible atmospherically resistant index’s strong temporal correlation with grain yield (up to 0.59) and plant height. The first 2 FPCAs explained 59.3 ± 13.9% and 74.2 ± 9.0% of the temporal variation of temporal data of VIs, respectively, facilitating predictions where flight times varied. Phenomic data, particularly when combined with genomic data, often were comparable to or numerically exceeded the base genomic model in prediction accuracy, particularly for grain yield in untested hybrids, although no significant differences in these models’ performance were consistently observed. Overall, this approach underscores the effectiveness of FPCA and combined models in enhancing the prediction of grain yield and plant height across environments and diverse agricultural settings.
Genome-wide association analyses using multilocus models on bananas (Musa spp.) reveal candidate genes related to morphology, fruit quality, and yield
Authors: Osorio-Guarin Jaime Andrés , Higgins Janet , Toloza-Moreno Deisy Lisseth , Di Palma Federica , Enriquez Valencia Ayda Lilia , Riveros Munévar Fernando , De Vega José J , Yockteng Roxana 
Published: May 2024

Abstract
Bananas (Musa spp.) are an essential fruit worldwide and rank as the fourth most significant food crop for addressing malnutrition due to their rich nutrients and starch content. The potential of their genetic diversity remains untapped due to limited molecular breeding tools. Our study examined a phenotypically diverse group of 124 accessions from the Colombian Musaceae Collection conserved in AGROSAVIA. We assessed 12 traits categorized into morphology, fruit quality, and yield, alongside sequence data. Our sequencing efforts provided valuable insights, with an average depth of about 7× per accession, resulting in 187,133 single-nucleotide polymorphisms (SNPs) against Musa acuminata (A genome) and 220,451 against Musa balbisiana (B genome). Population structure analysis grouped samples into four and five clusters based on the reference genome. By using different association models, we identified marker–trait associations (MTAs). The mixed linear model revealed four MTAs, while the Bayesian-information and linkage-disequilibrium iteratively nested keyway and fixed and random model for circulating probability unification models identified 82 and 70 MTAs, respectively. We identified 38 and 40 candidate genes in linkage proximity to significant MTAs for the A genome and B genome, respectively. Our findings provide insights into the genetic underpinnings of morphology, fruit quality, and yield. Once validated, the SNP markers and candidate genes can potentially drive advancements in genomic-guided breeding strategies to enhance banana crop improvement.
How repeated exposure to persuasive messaging shapes message responses over time: a longitudinal experiment
Authors: Skurka Chris , Keating David M 
Published: May 2024

Abstract
Repeated exposure theories have articulated several processes that explain how message repetition shapes persuasion over time, yet these processes are often studied in isolation. Moreover, repeated exposure theories have largely failed to specify the temporal trajectories of negative message responses over time. Integrating theorizing on repeated exposure, psychological reactance, and message fatigue, we conducted a mixed-design experiment (N = 1,416 total observations), varying the amount of daily exposure audiences had to campaign advertisements about distracted driving over nearly 2 weeks. Exposure to these messages (compared to control messages) led to greater message elaboration and reactance but not fatigue or attitudes. We found no evidence that these message reactions shifted linearly, logarithmically, or quadratically over time, with the exception that anger increased linearly with subsequent exposures. These findings suggest constraints on predictions made by repeated exposure frameworks, and post hoc analyses underscore the need to distinguish fatigue’s two dimensions conceptually and operationally.
Coping with alpine habitats: genomic insights into the adaptation strategies of Triplostegia glandulifera (Caprifoliaceae)
Authors: Zhang Jian , Dong Kai-Lin , Ren Miao-Zhen , Wang Zhi-Wen , Li Jian-Hua , Sun Wen-Jing , Zhao Xiang , Fu Xin-Xing , Ye Jian-Fei , Liu Bing , Zhang Da-Ming , Wang Mo-Zhu , Zeng Gang , Niu Yan-Ting , Lu Li-Min , Su Jun-Xia , Liu Zhong-Jian , Soltis Pamela S , Soltis Douglas E , Chen Zhi-Duan 
Published: May 2024

Abstract
How plants find a way to thrive in alpine habitats remains largely unknown. Here we present a chromosome-level genome assembly for an alpine medicinal herb, Triplostegia glandulifera (Caprifoliaceae), and 13 transcriptomes from other species of Dipsacales. We detected a whole-genome duplication event in T. glandulifera that occurred prior to the diversification of Dipsacales. Preferential gene retention after whole-genome duplication was found to contribute to increasing cold-related genes in T. glandulifera. A series of genes putatively associated with alpine adaptation (e.g. CBFs, ERF-VIIs, and RAD51C) exhibited higher expression levels in T. glandulifera than in its low-elevation relative, Lonicera japonica. Comparative genomic analysis among five pairs of high- vs low-elevation species, including a comparison of T. glandulifera and L. japonica, indicated that the gene families related to disease resistance experienced a significantly convergent contraction in alpine plants compared with their lowland relatives. The reduction in gene repertory size was largely concentrated in clades of genes for pathogen recognition (e.g. CNLs, prRLPs, and XII RLKs), while the clades for signal transduction and development remained nearly unchanged. This finding reflects an energy-saving strategy for survival in hostile alpine areas, where there is a tradeoff with less challenge from pathogens and limited resources for growth. We also identified candidate genes for alpine adaptation (e.g. RAD1, DMC1, and MSH3) that were under convergent positive selection or that exhibited a convergent acceleration in evolutionary rate in the investigated alpine plants. Overall, our study provides novel insights into the high-elevation adaptation strategies of this and other alpine plants.
Predictive Factors of Inactive Patient Participation in an Inflammatory Bowel Disease Learning Health System: A Longitudinal Cohort Study
Authors: Dang Nhu , Kumar Soryan , Kwon Michelle , Cradeur Michael , Zeven Katherine , Khunte Mihir , Marino Daniel , Tse Chung Sang , van Deen Welmoed K , Lee Adrian , Kamp Kendra , Oberai Ridhima , Melmed Gil Y , Siegel Corey A , Weaver S Alandra , Shah Samir A 
Published: May 2024

Lay Summary
Patient participation is crucial to learning health systems that leverage patient data to improve care practices. Age, history of anxiety or depression, and frequency of clinic visits were associated with inactive participation in an inflammatory bowel disease learning health system.
External stenting for saphenous vein grafts reduces early postoperative graft failure
Authors: Dushaj Stak , Häussler Achim , Rings Laura , Ntinopoulos Vasileios , Papadopoulos Nestoras , Odavic Dragan , Rodriguez Cetina Biefer Hector , Dzemali Omer 
Published: May 2024

Abstract

OBJECTIVES
Multiple studies have shown that external stenting (ExSt) mitigates the progression of vein graft disease years after coronary artery bypass grafting (CABG). We used computed tomography to evaluate the effect of ExSt on perioperative vein graft patency.

METHODS
This study assessed graft patency rates of saphenous vein grafts (SVG) in consecutive patients with isolated coronary artery bypass grafting (CABG) between 2018 and 2021. Logistic regression analyses were conducted to compare the outcomes of supported and non-supported groups at both patient and graft levels, with age, EuroSCORE II, gender, diabetes and arterial grafts as covariates. Subgroup analyses were performed based on different covariates. The goal of the study was to provide valuable insights into the clinical outcomes of SVG in patients having CABG.

RESULTS
The study examined a total of 357 patients who met the inclusion criteria and evaluated 572 vein grafts. Of these, 150 patients (205 SVGs) received ExSt, whereas 207 patients (337 SVGs) did not receive ExSt. The study results indicated that the likelihood of overall SVG patency at discharge was higher in the stented group than in the non-stented group, both at the level of the grafts [93.8% vs 87.8%, odds ratio (OR) 2.1; 95% confidence interval (CI) 1.0–4.5; P = 0.05] and at the patient level (90.1% vs 83.5%, OR 1.8; 95% CI 0.9–3.6; P = 0.1). It is worth noting that the difference between the stented and non-stented groups was most significant in the subgroup that received 2 arterial grafts (96.5% vs 89.6%, OR 3.2; 95% CI 1.2–8.4; P = 0.02) and in the subgroup with a higher EuroSCORE II (median &gt;1.1) (98.6% vs 88.6%, OR 8.8; 95% CI 1.1–72.7; P = 0.04).

CONCLUSIONS
The ExSt is associated with improved perioperative SVG patency at both the graft and the patient levels. Moreover, SVGs to the right territory and high-risk patients appear to have an advantage using ExSt.
How to harvest the left internal mammary artery—a randomized controlled trial
Authors: Laugesen Sofie , Krasniqi Lytfi , Benhassen Leila Louise , Mortensen Poul Erik , Pallesen Peter Appel , Bak Søren , Kjelsen Bo Juel , Riber Lars Peter 
Published: May 2024

Abstract

OBJECTIVES
It is uncertain whether Thunderbeat has a place in harvesting the left internal mammary artery (LIMA) and whether skeletonization is superior to pedicle-harvested LIMA. Some investigations have shown improved flowrates in the skeletonized graft. The aim of this study was to compare 3 groups of harvesting techniques: Pedicled, surgical skeletonized and skeletonized with Thunderbeat in terms of flow rates in the LIMA and postoperative in-hospital outcomes.

METHODS
Patients undergoing coronary artery bypass grafting with the LIMA to the anterior descending artery were randomized to pedicled (n = 56), surgical skeletonized (n = 55) and skeletonized with Thunderbeat (n = 54). Main outcomes were blood flow and pulsatility index in the graft.

RESULTS
No statistical difference between groups regarding flow in LIMA or pulsatility index. Similarly, no difference in postoperative bleeding or days of hospitalization. The duration of harvesting was faster for the pedicled technique compared with surgical skeletonized and skeletonized with Thunderbeat [mean total min: pedicled 20.2 min standard deviation (SD) ± 5.4; surgical skeletonized 28.6 min SD ± 8.7; skeletonized with Thunderbeat 28.3 min SD ± 9.11, P &lt; 0.001]. No grafts discarded due to faulty harvesting and there was no graft failure within hospital stay.

CONCLUSIONS
We found no difference between the harvesting methods except for a significantly faster harvesting time with the pedicled technique. However, non-touch skeletonized LIMA harvesting with Thunderbeat seems to be an effective alternative to traditional surgical skeletonized LIMA. The future will reveal whether patency is harvesting dependent.

Clinical trial registration
ClinicalTrials.gov Identifier: NCT05562908.