bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longitudinal management with crossmatch‐compatible platelets for refractory patients: alloimmunization, response to transfusion, and clinical outcomes (CME)
Authors: Wiita Arun P. , Nambiar Ashok 
Published: March 2012

BACKGROUND: The use of crossmatch‐compatible platelets (PLTs) improves posttransfusion corrected count increments (CCIs) in patients with alloimmune PLT refractoriness. However, few reports address the efficacy of utilizing this strategy for patients requiring intensive PLT transfusion therapy lasting several weeks to months.STUDY DESIGN AND METHODS: Medical records of patients with two or more PLT crossmatch assays performed between 2002 and 2010 were reviewed. All patients were refractory to random single‐donor apheresis PLT units, defined as two consecutive 1‐hour posttransfusion CCIs of less than 7500. A commercial solid‐phase adherence assay was used for crossmatching.RESULTS: Seventy‐one patients were included. A median of four crossmatch assays were performed per patient (range, 2‐17). Mean percent reactivity in initial (58.6%) versus last (55.3%) crossmatch assay for each patient demonstrated no trend toward progressive alloimmunization (p = NS). A total of 738 crossmatched PLT units were administered with a mean ± standard deviation CCI of 7000 ± 7900 (n = 443 units with adequate 1‐hr posttransfusion counts), a significant improvement over random PLTs (p < 0.001). Patients with an initial crossmatch reactivity of greater than 66% were significantly more likely to demonstrate at least one panreactive crossmatch assay, impacting the availability of compatible PLTs for optimum transfusion support. One patient (1.4%) developed WHO Grade IV bleeding.CONCLUSIONS: Progressive alloimmunization to mismatched antigens does not impact medium‐term transfusion support with crossmatched PLTs. Increased reactivity in the initial crossmatch assay can serve as a trigger to initiate workup for HLA‐matched PLTs as a second‐line approach. However, for most patients, medium‐term transfusion support with crossmatched PLTs offers an effective and rapid first‐line approach to management of PLT transfusion refractoriness.
Hemotherapy bedside biovigilance involving vital sign values and characteristics of patients with suspected transfusion reactions associated with fluid challenges: can some cases of transfusion‐associated circulatory overload have proinflammatory aspects?
Authors: Andrzejewski Chester , Popovsky Mark A. , Stec Theresa C. , Provencher Jean , O'Hearn Lynne , Visintainer Paul , Steingrub Jay 
Published: March 2012

BACKGROUND: Monitoring of patients' vital sign values (VSVs) during hemotherapy may have an important role in the recognition and mitigation of transfusion‐associated circulatory overload (TACO). Knowledge regarding VSVs and other patient characteristics in bedside‐reported TACO or fluid challenge–suspected transfusion reactions (TACO/FC‐STRs) is limited.STUDY DESIGN AND METHODS: We performed a retrospective observational cohort study of cases of uncomplicated red blood cell (RBC) transfusions (UCTs) and reported suspected transfusion reaction (STR) cases investigated by our hospital's transfusion medicine service (TMS) from January 1, 2005, to February 29, 2008, using data obtained from TMS consult reports and quality improvement databases examining VSVs and patient characteristics in TACO/FC‐STRs.RESULTS: The frequency of TACO/FC‐STRs was 0.19% per all RBC units transfused (1:530 units transfused). Both clinically and statistically (p ≤ 0.05) significant changes were encountered in all VSVs in patients experiencing TACO/FC‐STRs either at the 15‐minute time interval or at the end‐of‐transfusion time points. Measured and derived VSVs related to the patients' blood pressure in the peritransfusion period were consistently increased. Approximately two‐thirds of TACO/FC‐STR patients also exhibited inflammatory related signs and symptoms at STR bedside presentation. Differences (all p ≤ 0.050) between UCT and TACO/FC‐STR cohorts were seen for patient mean weights (80 kg vs. 72 kg), mean minutes to transfusion completion (121 min vs. 83 min), and mean storage age of suspected sentinel RBC unit (22.5 days vs. 25.2 days).CONCLUSION: Trend monitoring of peritransfusion VSVs, especially blood pressures, may aid in the bedside recognition of TACO/FC‐STRs. A subset of these patients may also present with febrile and/or inflammatory signs and symptoms.
An overlooked aspect on metabolic acidosis at birth: Blood gas analyzers calculate base deficit differently
Authors: MOKARAMI PARISA , WIBERG NANA , OLOFSSON PER 
Published: March 2012

Abstract Objective. Metabolic acidosis (MA) at birth is commonly defined as umbilical cord arterial pH < 7.0 plus base deficit (BD) ≥ 12.0 mmol/L. Base deficit is not a measured entity but is calculated from pH and Pco2 values, with the hemoglobin (Hb) concentration [Hb] included in the calculation algorithm as a fixed or measured value. Various blood gas analyzers use different algorithms, indicating variations in the MA diagnosis. The objective was therefore to calculate the prevalence of MA in blood and extracellular fluid with algorithms from three blood gas analyzer brands relative to the Clinical and Laboratory Standards Institute (CLSI) algorithm. Design. Comparative study. Setting. University hospital. Sample. Arterial cord blood from 15 354 newborns. Main outcome measure. Prevalence of MA. Methods. Blood was analyzed in a Radiometer ABL 735 analyzer. Base deficit was calculated post hoc with algorithms from CLSI and Corning and Roche blood gas analyzers, and with measured and fixed (9.3 mmol/L) values of [Hb]. Results. The prevalence of BD ≥12.0 mmol/L in blood was with the CLSI algorithm 1.97%, Radiometer 5.18%, Corning 3.84% and Roche 3.29% (CLSI vs. other; McNemar test, p < 0.000001). Likewise, MA prevalences were 0.58, 0.66, 0.64 and 0.64%, respectively (p≤ 0.02). Base deficit ≥ 12.0 mmol/L and MA rates were lower in extracellular fluid than in blood (p≤ 0.002). Algorithms with measured or fixed Hb concentration made no differences to MA rates (p≥ 0.1). Conclusions. The neonatal metabolic acidosis rate varied significantly with blood gas analyzer brand and fetal fluid compartment for calculation of BD.
Primary cutaneous CD56 positive lymphoma: a diagnostic conundrum in an unusual case of lymphoma
Authors: Schieke Stefan M. , Sharaf Mohammed A. , Lerner Adam , Rünger Thomas M. , Mahalingam Meera 
Published: March 2012

We present an unusual case of a CD56‐positive T‐cell lymphoma exhibiting immunophenotypic characteristics of both γδ T‐cell lymphoma and extranodal NK/T‐cell lymphoma, nasal‐type. The patient presented with a 2‐month history of rapidly progressive, pruritic and cutaneous nodules on his arms. A biopsy showed a dense pan‐dermal infiltrate of markedly atypical CD3‐positive lymphocytes, compatible with tumor stage cutaneous T‐cell lymphoma. Retrospective review of a preceding biopsy and flow cytometric analysis, performed at an outside institution, showed strong expression of surface CD3, CD7, CD43 and γδ T‐cell receptor (TCR), findings consistent with a diagnosis of cutaneous γδ T‐cell lymphoma. In light of these data, we performed additional studies that showed diffuse positive staining of the atypical lymphocytes for CD56, CD4 and CD43 as well as Epstein–Barr virus‐encoded small nonpolyadenylated RNA (EBER). Interestingly, this case displays characteristic features of γδ T‐cell lymphoma, with strong surface expression of CD3 and γδ‐TCR, as well as characteristics of natural killer (NK)/T‐cell lymphoma, including expression of CD4 and EBER positivity, that represent two separate categories in the current classification of cutaneous lymphomas. Taken together, these findings underscore the difficulty of rendering an unambiguous classification of the presented neoplasm given the close ontogenetic relationship between NK and cytotoxic T‐cells and highlight the need for continued reevaluation of the current classification system.Schieke SM, Sharaf MA, Lerner A, Rünger TM, Mahalingam M. Primary cutaneous CD56 positive lymphoma: a diagnostic conundrum in an unusual case of lymphoma.
Enamel defects associated with coeliac disease: putative role of antibodies against gliadin in pathogenesis
Authors: Muñoz Florencia , Del Río Natalia , Sóñora Cecilia , Tiscornia Inés , Marco Alicia , Hernández Ana 
Published: March 2012

Muñoz F, Del Río N, Sóñora C, Tiscornia I, Marco A, Hernández A. Enamel defects associated with coeliac disease: putative role of antibodies against gliadin in pathogenesis.

Eur J Oral Sci 2012; 120: 104–112. © 2012 Eur J Oral SciEnamel defects in the permanent teeth of patients with coeliac disease (CD) are often reported as an atypical manifestation, sometimes being suggestive of an undiagnosed atypical disease. We proposed to explore the pathogenesis of these oral defects, which are poorly studied. Sequence analyses of proteins from gluten (gliadins) and of proline‐rich enamel proteins (amelogenin and ameloblastin) suggested the presence of common antigenic motifs. Therefore, we analyzed, by ELISA and western blotting, the reactivity of sera from patients with CD against gliadin and enamel‐derived peptides. Correlation analyses between the levels of specific antibodies against gliadin and enamel derived peptides and inhibition experiments confirmed the presence of cross‐reactive antibodies. Immunoblot analysis revealed that the most prominent component in enamel matrix derivative (of approximately 18.6 kDa), identified by an amelogenin‐specific antibody, is recognized by sera from patients with CD; in addition, several fractions of pure gliadin were recognized by amelogenin‐specific antibody. In agreement, sera from mice immunized with enamel matrix‐derived proteins generated antibodies that recognized a peptide (of approximately 21.2 kDa) derived from gliadin. In conclusion, antibodies against gliadin generated in patients with CD can react in vitro with a major enamel protein. The involvement of anti‐gliadin serum in the pathogenesis of enamel defects in children with untreated CD can be hypothesized on the basis of these novel results.
Storms of Tears. Emotion Metaphors and the Construction of Gender in East Lynne
Authors: Hansson Heidi , Norberg Cathrine 
Published: March 2012

Despite the fact that the consequences of emotional actions are a central theme in British nineteenth‐century sensation fiction, studies of the genre have generally focused on thrilling and socially disruptive elements of the genre. Attention to descriptions of transgressive behaviour and in particular women’s violations of patriarchal norms has enabled interpretations that endow the genre with a feminist agenda. Reading the novels with a focus on how emotions are represented, however, demonstrates an underlying patriarchal pattern that limits the potential for feminist interpretations.This article focuses on the clusters of metaphors used to express emotion in Ellen Wood’s sensation novel East Lynne (1861–1862). Close readings of text examples in combination with analyses of metaphorical expressions highlight the novel’s ideological ambiguities and ambivalences concerning gender and emotion.
Michel de Ghelderode, the Wooster Group, and postmodernist revisions of La tentation de saint Antoine
Authors: Thomas Andrea S. 
Published: March 2012

La tentation de saint Antoine initially recalls Hieronymus Bosch’s famous Renaissance painting or Flaubert’s onieric work‐in‐progress. A closer look at more recent adaptations of the story of Saint Anthony the Great, however, reveals that such transformations of the myth addressed shifting aesthetics in the twentieth century by combining genres and texts, designed to cast what Foucault considered Flaubert’s most modernist ideas in an entirely postmodern light. This paper examines two of these adaptations: first, Michel de Ghelderode’s multiple versions of the tale set in Flanders, published separately from 1919 to 1932, but not performed until 1957 as a radio play and marionette show; and, second, the Wooster Group’s provocative stage adaptation, which transplanted the hermit Anthony to an excess‐filled New York of the eighties in Frank Dell’s The Temptation of Saint Antony.What Flaubert created as a self‐reflexive commentary on the dangers of books, twentieth‐century adaptors turned into a postmodern statement about other contemporary media forms, including stage, radio, cinema, and television. More importantly, by strategically appropriating not only Flaubert’s subject matter, but also his composition technique and passages from his texts, these artists draw attention to the perils of a culture‐saturated twentieth century in which art had become adaptation.
Effects of cigarette smoke and nicotine on cell viability, migration and myofibroblastic differentiation
Authors: Silva D. , Cáceres M. , Arancibia R. , Martínez C. , Martínez J. , Smith P. C. 
Published: March 2012

Silva D, Cáceres M, Arancibia R, Martínez C, Martínez J, Smith P. Effects of cigarette smoke and nicotine on cell viability, migration and myofibroblastic differentiation. J Periodont Res 2012; 47: 599–607. © 2012 John Wiley & Sons A/SBackground and Objective:  Several studies have analysed the role of nicotine as a prominent agent affecting wound repair in smokers. However, tobacco smoke contains several components that may alter gingival wound healing. The present study aimed to analyse the roles of cigarette smoke condensate (CSC) and nicotine on cell viability, cell migration/invasion and myofibroblastic differentiation using primary cultures of human gingival fibroblasts.Material and Methods:  To compare the effects of CSC and nicotine, gingival fibroblasts were stimulated with CSC (0.4–500 μg/mL) and the corresponding nicotine concentrations (0.025–32 μg/mL) present in research cigarettes (1R3F). Cell viability was evaluated through the MTS assay. Cell migration and invasion were assessed through scratch wound assays, collagen nested matrices and transwell migration. α‐Smooth muscle actin production was evaluated by western blotting.Results:  Cigarette smoke condensate at 50 μg/mL induced a moderate increase in cell viability, whereas the corresponding nicotine concentration (3.2 μg/mL) did not produce this response. Cigarette smoke condensate at 250 μg/mL, but not nicotine at 16 μg/mL (the corresponding nicotine concentration), induced cell death. Both nicotine and CSC stimulated cell migration (50 μg/mL CSC; 3.2 μg/mL nicotine). At 150 μg/mL, CSC inhibited cell migration; however, the corresponding concentration of nicotine (9.6 μg/mL), did not have this effect. Although both nicotine and CSC inhibited α‐smooth muscle actin production, only the latter induced a statistically significant effect on this response.Conclusion:  Cigarette smoke condensate may stimulate cell survival and migration at low concentrations and inhibit these cell responses at higher levels of exposure. Moreover, CSC may interfere in myofibroblastic differentiation. These results show that cigarette smoke, but not nicotine, may significantly alter cell viability, cell migration and myofibroblastic differentiation in gingival mesenchymal cells.
A Systematic Review of the Effectiveness of Videoconference‐Based Tele‐Education for Medical and Nursing Education
Authors: Chipps Jennifer , Brysiewicz Petra , Mars Maurice 
Published: March 2012

ABSTRACTBackground: Rural nurses and doctors typically have little opportunity to further their education and training. Studies have shown high participant satisfaction with the use of educational technology, such as videoconferencing, for education. A review of effectiveness of videoconference‐based tele‐education for medical and nursing education was conducted.Aims: The aims of this study were to: (1) systematically review the literature and critique the research methods on studies addressing the review question: “How effective is videoconference‐based education for the education of doctors and nurses?” (2) summarize the existing evidence on the effectiveness of videoconference education for medical and nursing staff; and (3) apply the findings to South Africa and other countries across the globe.Methods: Research citations from 1990 to 2011 from cumulative index of nursing and allied health literature, Medline, Pubmed, PsycInfo, EBSCOhost, SABINET, Cochrane Database of Systematic Reviews, the Cochrane Controlled Trial Registry, Database of Abstracts of Reviews of Effectiveness, unpublished abstracts through NEXUS and Internet search engines (Google/Google scholar) were searched. Review methods included searching, sifting, abstraction, and quality assessment of relevant studies by two reviewers. Studies were evaluated for sample, design, intervention, threats to validity, and outcomes. No meta‐analysis was conducted as the studies provided heterogeneous outcome data.Results: Five studies were reviewed. Videoconference and face‐to‐face education is at least equivalent and one study reported an increase in knowledge and knowledge integration.Recommendations: Despite the methodological limitations and heterogeneity of the reviewed studies, there appears to be sufficient evidence of effectiveness to provide a rigorous Grade B evidence‐based recommendation of moderate support.Conclusions: The use of videoconferencing for nursing and medical education should be encouraged along with guidelines for the use of videoconferencing. The paucity of studies and the lack of empirical precision in evaluating effectiveness of these interventions necessitate future rigorously designed experimental studies.
Guillain–Barre syndrome in colorectal cancer
Authors: VATANDOUST Sina , JOSHI Rohit , PRICE Timothy J 
Published: March 2012

AbstractGuillain–Barré syndrome has been reported in the setting of different malignancies. To the best of our knowledge, the association of Guillain–Barré syndrome and colorectal cancer has been reported in only two cases. As Guillain–Barré syndrome is potentially life threatening, it should be considered in the differential diagnosis of patients with colorectal cancer with neurological findings. Here we report two cases of Guillain–Barré syndrome in the setting of metastatic colorectal cancer.
Upfront maintenance therapy with arsenic trioxide in acute promyelocytic leukemia provides no benefit for non‐t(15;17) subtype
Authors: CHIANG Yi‐Hao , CHANG Yi‐Fang , HSIEH Ruey‐Kuen , LIN Johnson , CHEN Caleb Gonshen , LIM Ken‐Hong , LIN Huan‐Chau , CHANG Ming‐Chih 
Published: March 2012

AbstractAims:  The optimal maintenance therapy for patients with acute promyelocytic leukemia (APL) who achieved complete remission (CR) and complete consolidation chemotherapy is still controversial. Whether the use of arsenic trioxide (ATO) alone or along with all‐trans retinoic acid (ATRA) improves overall survival (OS) or disease‐free survival (DFS) is still debated.Methods:  A retrospective reivew was conducted of 20 patients diagnosed with APL according to the French – American – British system. After achieving CR and receiving consolidation chemotherapy, nine patients were given maintenance therapy for 1 year (ATRA 45 mg/m2/day p.o., mercaptopurine 60 mg/m2/day p.o. and ATO 0.15 mg/kg/day × 5 days/week for six cycles in five patients; ATRA 45 mg/m2/d p.o. alternating with ATO 0.15 mg/kg/day × 5 days/week in 1 patient; ATRA only in three patients).Results:  In all patients the rates of CR, 3‐year OS and 5‐year OS were 75, 71 and 57%, respectively. For patients treated with ATO maintenance, the rates were 100% for both 5‐year OS and 5‐year DFS. Four of six patients on ATO maintenance had grade 1 or grade 2 adverse events. Excluding the two patients who died from intracerebral hemorrhage within 4 days after diagnosis, these rates were 85, 82 and 78%, respectively.Conclusion:  Upfront ATO maintenance therapy for one year is safe and appears to be effective, with the benefits restricted to patients with APL with t(15;17) translocation. Larger studies will be required to confirm this observation.
Impact of body mass index and visceral adiposity on outcomes in colorectal cancer
Authors: YAMAMOTO Naoto , FUJII Shoichi , SATO Tsutomu , OSHIMA Takashi , RINO Yasushi , KUNISAKI Chikara , MASUDA Munetaka , IMADA Toshio 
Published: March 2012

AbstractAim:  Obesity and visceral obesity are closely related to the development of colorectal cancer, as well as other metabolic complications. We investigated the prognostic significance of body mass index (BMI) and visceral obesity in 273 patients with resectable colorectal cancer.Methods:  Visceral fat area (VFA) and subcutaneous fat area were measured on digital images of patients’ computed tomograms obtained before surgery. The patients were divided into two groups according to the cut‐off levels of VFA proposed by Oka et al. Men with a VFA of ≥130 cm2 and women with a VFA of ≥90 cm2 were classified as obese (VFA‐obese) and the others were classified as non‐obese (VFA‐non‐obese). The patients were also divided into an overweight group and a normal range group, according to their preoperative BMI.Results:  There was no significant difference in cumulative recurrence‐free survival (RFS) or overall survival (OS) between the VFA‐obese group and the VFA‐non‐obese group. In the subgroup of patients with Dukes’ C disease (n = 100) there was no statistically significant difference in RFS and OS between the VFA‐obese group and the VFA‐non‐obese group. The results were similar when the patients were classified according to their BMI.Conclusion:  Neither obesity nor increased visceral adiposity has any influence on outcomes in patients with resectable colorectal cancer.
Discovery of the novel HLA‐DRB1*10:04 allele in a Taiwanese volunteer bone marrow donor and identification of the probable HLA‐A, ‐B, ‐C and ‐DRB1 haplotype in association with DRB1*10:04
Authors: Yang K. L. , Lee S. K. , Kao R. H. , Lin C. L. , Lin P. Y. 
Published: March 2012

SummaryWe report here a novel variant of HLA‐DRB1*10, DRB1*10:04, discovered in a Taiwanese volunteer bone marrow donor by a sequence‐based typing (SBT) method. The DNA sequence of DRB1*10:04 differs from DRB1*10:01:01, in exon 2, at nucleotide positions 296 (G→A) and 303 (T→G). The nucleotide changes caused an amino acid substitution at amino acid residue 70 (R→Q). We hypothesize that the formation of DRB1*10:04 was probably the result of a gene recombination event where DRB1*10:01:01 received a minimum length of DNA sequence from DRB1*04:05:01, as the sequence of DRB1*10:04 is identical to DRB1*10:01:01 in exon 2 except the sequence from nucleotide 296 to nucleotide 303, which is identical to DRB1*04:05:01. The plausible HLA‐A, ‐B, ‐C and ‐DRB1 haplotypes in association with DRB1*10:04 was deduced as A*01:01‐B*37:01‐C*06:02‐DRB1*10:04.
Recent Progress Using High‐throughput Sequencing Technologies in Plant Molecular BreedingF
Authors: Gao Qiang , Yue Guidong , Li Wenqi , Wang Junyi , Xu Jiaohui , Yin Ye 
Published: March 2012

Abstract
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[ Ye Yin ]High‐throughput sequencing is a revolutionary technological innovation in DNA sequencing. This technology has an ultra‐low cost per base of sequencing and an overwhelmingly high data output. High‐throughput sequencing has brought novel research methods and solutions to the research fields of genomics and post‐genomics. Furthermore, this technology is leading to a new molecular breeding revolution that has landmark significance for scientific research and enables us to launch multi‐level, multi‐faceted, and multi‐extent studies in the fields of crop genetics, genomics, and crop breeding. In this paper, we review progress in the application of high‐throughput sequencing technologies to plant molecular breeding studies.