bluuebunny/crossref_metadata_2025_split huggingface dataset:
Storage conditions affect the composition of the lyophilized secretome of multipotent mesenchymal stromal cells
Authors: Rogulska Olena , Vackova Irena , Prazak Simon , Turnovcova Karolina , Kubinova Sarka , Bacakova Lucie , Jendelova Pavla , Petrenko Yuriy 
Published: May 2024

AbstractThe widespread use of multipotent mesenchymal stromal cell-derived secretome (MSC-sec) requires optimal preservation methods. Lyophilization offers benefits like concentrating the secretome, reducing the storage volume, and making storage conditions more flexible. This study evaluated the influence of storage duration and temperature on lyophilized MSC-sec. The conditioned medium from Wharton’s jelly MSCs was stored at – 80 °C or lyophilized with or without trehalose. Lyophilized formulations were kept at – 80 °C, − 20 °C, 4 °C, or room temperature (RT) for 3 and 30 months. After storage and reconstitution, the levels of growth factors and cytokines were assessed using multiplex assay. The storage of lyophilized MSC-sec at – 80 °C ensured biomolecule preservation for 3 and 30 months. Following 3 month storage at 4 °C and RT, a notable decrease occurred in BDNF, bNGF, and sVCAM-1 levels. Prolonged 30 month storage at the same temperatures significantly reduced BDNF, bNGF, VEGF-A, IL-6, and sVCAM-1, while storage at – 20 °C decreased BDNF, bNGF, and VEGF- A levels. Trehalose supplementation of MSC-sec improved the outcome during storage at 4 °C and RT. Proper storage conditions were crucial for the preservation of lyophilized MSC-sec composition. Short-term storage at various temperatures maintained over 60% of the studied growth factors and cytokines; long-term preservation was only adequate at −80 °C.
Inferior vena cava filter use at a large community hospital: a retrospective cohort study
Authors: Fontyn Stephanie , Bai Yuxin , Bolger Samantha , Greco Kaity , Wang Tzu-Fei , Hamm Caroline , Cervi Andrea 
Published: May 2024

AbstractInferior vena cava (IVC) filters are considered when patients with venous thromboembolism (VTE) develop a contraindication to anticoagulation. Use of IVC filters is increasing, despite associated complications and lack of data on efficacy in reducing VTE-related mortality. We characterized the pattern of IVC filter use at a large community hospital between 2018 and 2022. Specifically, we assessed the indications for IVC filter insertion, filter removal rates, and filter-associated complications. Indications for IVC filters were compared to those outlined by current clinical practice guidelines. We reviewed 120 consecutive filter placement events. The most common indications included recent VTE and active bleeding (40.0%) or need for anticoagulation interruption for surgery (25.8%). Approximately one-third (30.0%) of IVC filters were inserted for indications either not supported or addressed by guidelines. Half (50.0%) of patients had successful removal of their IVC filter. At least 13 patients (10.8%) experienced a filter-related complication. In a large community-based practice, nearly one-third of IVC filters were inserted for indications not universally supported by current practice guidelines. Moreover, most IVC filters were not removed, raising the risk of filter-associated complications, and supporting the need for development of comprehensive guidelines addressing use of IVC filters, and post-insertion monitoring practices.
Dynamic molecular signatures of acute myocardial infarction based on transcriptomics and metabolomics
Authors: Wang Xuejiao , Yang Guang , Li Jun , Meng Chao , Xue Zengming 
Published: May 2024

AbstractAcute myocardial infarction (AMI) commonly precedes ventricular remodeling, heart failure. Few dynamic molecular signatures have gained widespread acceptance in mainstream clinical testing despite the discovery of many potential candidates. These unmet needs with respect to biomarker and drug discovery of AMI necessitate a prioritization. We enrolled patients with AMI aged between 30 and 70. RNA-seq analysis was performed on the peripheral blood mononuclear cells collected from the patients at three time points: 1 day, 7 days, and 3 months after AMI. PLC/LC–MS analysis was conducted on the peripheral blood plasma collected from these patients at the same three time points. Differential genes and metabolites between groups were screened by bio-informatics methods to understand the dynamic changes of AMI in different periods. We obtained 15 transcriptional and 95 metabolite expression profiles at three time points after AMI through high-throughput sequencing. AMI-1d: enrichment analysis revealed the biological features of 1 day after AMI primarily included acute inflammatory response, elevated glycerophospholipid metabolism, and decreased protein synthesis capacity. Phosphatidylcholine (PC) and phosphatidylethanolamine (PE) might stand promising biomarkers to differentiate post-AMI stage. Anti-inflammatory therapy during the acute phase is an important direction for preventing related pathology. AMI-7d: the biological features of this stage primarily involved the initiation of cardiac fibrosis response and activation of platelet adhesion pathways. Accompanied by upregulated TGF-beta signaling pathway and ECM receptor interaction, GP5 help assess platelet activation, a potential therapeutic target to improve haemostasis. AMI-3m: the biological features of 3 months after AMI primarily showed a vascular regeneration response with VEGF signaling pathway, NOS3 and SHC2 widely activated, which holds promise for providing new therapeutic approaches for AMI. Our analysis highlights transcriptional and metabolomics signatures at different time points after MI, which deepens our understanding of the dynamic biological responses and associated molecular mechanisms that occur during cardiac repair.
Enhancement of thermal energy transfer behind a double consecutive expansion utilizing a variable magnetic field
Authors: Bahrami Hamid-Reza , Ghaedi Mahziyar 
Published: May 2024

AbstractThis research focuses on utilizing non-uniform magnetic fields, induced by dipoles, to control and enhance thermal energy transfer in a two-dimensional cooling conduit including a double backward-facing step. The presence of electronic equipment along the straight channel path creates such arrangements, and cooling is often ineffective in the corners of the formed steps. The use of a non-constant magnetic field is a passive technique to improve the cooling rate in these sections without changing the internal geometry, thereby increasing the heat transfer rate. A commercial software based on the finite volume technique is employed to solve the governing equations of fluid flow and heat transfer. Multiple parameters are examined in this study, including the flow Reynolds number (12.5–50), dipole location and strength (0.1–5 A-m), and the number of dipoles (single or double). The results indicate that all of these parameters have a significant impact on the thermal energy transfer. The results of the study show that a single dipole increase the average heat transfer by about 22%, two magnetic fields by 40%, the strength of the magnetic source by 24% with respect to the non-magnetic field in the present study.
Follicle-stimulating hormone receptor expression in advanced atherosclerotic plaques
Authors: Ghinea Nicolae , Liehn Elisa Anamaria , Grommes Jochen , Delattre Diane Dalila , Olesen Tine Kold 
Published: May 2024

AbstractExperimental evidence indicates that follicle-stimulating hormone (FSH), an essential hormone for reproduction, can act directly on endothelial cells inducing atherosclerosis activation and development. However, it remains unknown whether the FSH-receptor (FSHR) is expressed in human atherosclerosis plaques. To demonstrate the FSHR presence, we used immunohistochemical and immunoelectron microscopy involving a specific monoclonal antibody FSHR1A02 that recognizes an epitope present in the FSHR-ectodomain. In all 55 patients with atherosclerotic plaques located in carotid, coronary, femoral arteries, and iliac aneurysm, FSHR was selectively expressed in arterial endothelium covering atherosclerotic plaques and endothelia lining intraplaque neovessels. Lymphatic neovessels were negative for FSHR. M1-macrophages, foam cells, and giant multinucleated cells were also FSHR-positive. FSHR was not detected in normal internal thoracic artery. Immunoelectron microscopy performed in ApoEKO/hFSHRKI mice with atherosclerotic plaques, after injection in vivo with mouse anti-hFSHR monoclonal antibody FSHR1A02 coupled to colloidal gold, showed FSHR presence on the luminal surface of arterial endothelial cells covering atherosclerotic plaques. Therefore, FSHR can bind, internalize, and deliver into the plaque circulating ligands to FSHR-positive cells. In conclusion, we report FSHR expression in endothelial cells, M1-macrophages, M1-derived foam cells, giant multinucleated macrophages, and osteoclasts associated with human atherosclerotic plaques.
Experimental study on failure mode and fracture evolution characteristics of red shale in Kaiyang Phosphorus mining area
Authors: Ma Zhenqian , Zhou Lang , Zuo Shaojie , Zhang Jimin 
Published: May 2024

AbstractIn order to study the failure mode and fracture evolution characteristics of red shale in Kaiyang Phosphorus mining area, conventional triaxial compression mechanical tests of red shale with different bedding dip angles were carried out by using DSTD-1000 electro-hydraulic servo rock mechanics experiment system. Based on the laboratory test results, the conventional triaxial particle flow simulation of red shale samples with different bedding dip angles was carried out using discrete element PFC2D. The results show that: (1) the failure mode of red shale is controlled by bedrock when the bedding dip angle is 0° and 60° ~ 90°. When the bedding dip angle is 15° ~ 45°, the rock failure mode is controlled by bedding. The compressive strength of rock is the minimum when the bedding dip angle is 30°and the maximum at 0°, which is about 2 times of the minimum. (2) In the failure process of red shale, the cracks with different bedding dip angles show slow growth stage, accelerated growth stage and stable stage with axial strain. The whole failure process is dominated by tensile cracks, accompanied by a few shear cracks. (3) The type of displacement field varies with the bedding dip angle: tensile failure and shear failure are the main displacement field types at 15° ~ 45°, and mixed failure is often the main mode at 60° ~ 90°and 0°. The research results provide the basis and reference for the safety control of red shale roadway.
Projected habitat preferences of commercial fish under different scenarios of climate change
Authors: Sharifian Sana , Mortazavi Mohammad Seddiq , Mohebbi Nozar Seyedeh Laili 
Published: May 2024

AbstractThe challenges of commercial species with the threats of climate change make it necessary to predict the changes in the distributional shifts and habitat preferences of the species under possible future scenarios. We aim to demonstrate how future climatic changes will affect the habitat suitability of three species of commercial fish using the predictive technique MaxEnt. The dataset used to extract geographical records included OBIS (54%), GBIF (1%), and literature (45%). The output of the model indicated accurate projections of MaxEnt (AUC above 0.9). Temperature was the main descriptor responsible for the main effects on the distribution of commercial fish. With increasing RCP from 2.5 to 8.5, the species would prefer saltier, higher temperatures and deeper waters in the future. We observed different percentages of suitable habitats between species during RCPs showing distinct sensitivity of each fish in facing climate changes. Negative effects from climate change on the distribution patterns of commercial fish were predicted to lead to varying degrees of reduction and changes of suitable habitats and movement of species towards higher latitudes. The finding emphasizes to implement adaptive management measures to preserve the stocks of these commercial fish considering that the intensification of the effects of climate change on subtropical areas and overexploited species is predicted.
Effect of the chromaticity of stimuli on night vision disturbances
Authors: Castro-Torres José J. , Casares-López Miriam , Ortiz-Peregrina Sonia , Martino Francesco , Gómez-Robledo Luis , Jiménez José R. 
Published: May 2024

AbstractThe perception of halos and other night vision disturbances is a common complaint in clinical practice. Such visual disturbances must be assessed in order to fully characterize each patient’s visual performance, which is particularly relevant when carrying out a range of daily tasks. Visual problems are usually assessed using achromatic stimuli, yet the stimuli encountered in daily life have very different chromaticities. Hence, it is important to assess the effect of the chromaticity of visual stimuli on night vision disturbances. The aim of this work is to study the influence of the chromaticity of different visual stimuli on night vision disturbances by analyzing straylight and visual discrimination under low-light conditions. For that, we assessed the monocular and binocular visual discrimination of 27 subjects under low illumination using the Halo test. The subjects’ visual discrimination was assessed after exposure to different visual stimuli: achromatic, red, green, and blue, both at the monitor’s maximum luminance and maintaining the same luminance value for the different visual stimuli. Monocular straylight was also measured for an achromatic, red, green, and blue stimuli. The blue stimulus had the greatest effect on halos in both monocular and binocular conditions. Visual discrimination was similar for the red, green, and achromatic stimuli, but worsened at lower luminance. The greatest influence of straylight was observed for the blue stimulus. In addition, visual discrimination correlated with straylight measurements for achromatic stimuli, wherein greater straylight values correlated with an increased perception of halos and other visual disturbances.
Bilingualism Predicts Affective Theory of Mind in Autistic Adults
Authors: Cummings Kaitlin K. , Greene Rachel K. , Cernasov Paul , Kan Dang Dang Delia , Parish-Morris Julia , Dichter Gabriel , Kinard Jessica L. 
Published: May 2024

Purpose:
This study examined the impact of bilingualism on affective theory of mind (ToM) and social prioritization (SP) among autistic adults compared to neurotypical comparison participants.

Method:
Fifty-two (25 autistic, 27 neurotypical) adult participants (ages 21–35 years) with varying second language (L2) experience, ranging from monolingual to bilingual, completed an affective ToM task. A subset of this sample also completed a dynamic eye-tracking task designed to capture differences in time spent looking at social aspects of a scene (SP). Four language groups were compared on task performance (monolingual autism and neurotypical, bilingual autism and neurotypical), followed by analyses examining the contribution of L2 experience, autism characteristics, and social face prioritization on affective ToM, controlling for verbal IQ. Finally, we conducted an analysis to identify the contribution of SP on affective ToM when moderated by autism status and L2 experience, controlling for verbal IQ.

Results:
The monolingual autism group performed significantly worse than the other three groups (bilingual autism, monolingual neurotypical, and bilingual neurotypical) on the affective ToM task; however, there were no significant differences between the bilingual autism group compared to the monolingual and bilingual neurotypical groups. For autistic individuals, affective ToM capabilities were positively associated with both verbal IQ and L2 experience but did not relate to autism characteristics or SP during eye tracking. Neurotypical participants showed greater SP during the eye-tracking task, and SP did not relate to L2 or autism characteristics for autistic individuals. SP and verbal IQ predicted affective ToM performance across autism and neurotypical groups, but this relationship was moderated by L2 experience; SP more strongly predicted affective ToM performance among participants with lower L2 experience (e.g., monolingual) and had less of an impact for those with higher L2 experience.

Conclusion:
This study provides support for a bilingual advantage in affective ToM for autistic individuals.

Supplemental Material:

https://doi.org/10.23641/asha.25696083
Untersuchungen zu einem kontrollierten, entscheidungsbaumbasierten
Verfahren des Selektiven Trockenstellens in Bayerischen
Milchviehbetrieben
Authors: Sonnewald-Daum Tanja , Euchner Katharina , Frost Laetitia , Pauly Tim , Fuchs Carla , Zettler Bianca , Schade Rebekka , Huber-Schlenstedt Reglindis , Harms Jan , Klima André , Mansfeld Rolf 
Published: May 2024

Zusammenfassung
Ziel Vier Parameter eines, in einer vorherigen Studie geprüften,
Entscheidungsbaumes (EnB) für das Selektive Trockenstellen (TS) wurden auf ihre
Selektionsstärke zur Erkennung von antibiotisch trockenzustellenden Kühen
(KTV/AB) geprüft. Ebenso, ob alle Parameter (Zellzahlen
[ZZ≥200 000 Z/ml] der letzten 3 Milchleistungsprüfungen [MLP] vor dem TS,
Mastitis-Historie der Laktation [≥1 MH], mikrobiologische Untersuchung [MBU, 14d
vor TS, Major Pathogen-Nachweis] und California-Mastitis-Test [CMT, >Grad
1/+, beim TS]) notwendig sind, Modifikationen als Ersatz der MBU sowie ein
vereinfachtes Modell anstatt des EnB ermittelt werden können.
Material und Methoden Daten aus 18 bayerischen Milchviehbetrieben von
06/2015 bis 08/2017 wurden deskriptiv sowie mittels kostensensitivem binärem
Klassifikationsbaum und logit-Modellen ausgewertet. Der EnB galt als zugrunde
liegende Wahrheit.
Ergebnisse Einbezogen wurden 848 Trockenstellvorgänge (KTV) von
739 Kühen. ZZ und CMT selektierten 88,1%, in Kombination mit der MH 95,6% der
KTV/AB (n=494). Ohne MBU wären 22 (4,4%) der KTV/AB
mit Major Pathogen-Nachweis (davon 8x Staphylococcus [S.]
aureus) fehlklassifiziert worden. Im Durchschnitt des geometrischen
Mittels der ZZ innerhalb 100d vor TS, blieben KTV ohne Befund in der
MBU <100 000 Z/ml Milch, mit Minor Pathogen zwischen 100 000–150 000 Z/ml,
mit Major Pathogen (ohne S. aureus) ≥150 000 Z/ml. Bei den 2
Selektionskriterien ZZ in der Laktation mind. 1x >200 000 Z/ml und postiver
CMT beim TS, wären 37 KTV(4,4%) „falsch nicht“, 43 KTV
(5,1%) „unnötig“ für ein antibiotisches TS selektiert worden. Modifikationen,
u. a. ZZ <131 000 Z/ml innerhalb 100d vor TS, zur Selektion von
KTV ohne Befund/mit Minor Pathogen, wurden ermittelt. Das beste
Modell zur Selektion der Trockenstellbehandlung (KTV ohne MH & ZZ
<200 000 Z/ml in den letzten 3 MLP) wies Metriken von AUC=0,74,
Accuracy=0,78, balanced Accuracy=0,63, Sensitivität=0,92 und Spezifität=0,33
auf.
Schlussfolgerungen ZZ, CMT und MH des EnB in Kombination erwiesen sich
unter den Bedingungen der vorliegenden Studie als geeignete Selektionskriterien.
Eine Herabsetzung der ZZ-Grenzen ist betriebsindividuell sinnvoll, wenn auf die
MBU verzichtet werden soll. Das beste Modell konnte den EnB nicht ersetzen.
Pflanzliche bäuerliche Hausmittel – Tradition mit
Praxisrelevanz
Authors: Schlittenlacher Theresa , Knubben-Schweizer Gabriela , Maeschli Ariane , Walkenhorst Michael 
Published: May 2024

Zusammenfassung
Ziel Die Herstellung von pflanzlichen bäuerlichen Hausmitteln wird seit
jeher von Tierhalter*innen in Eigenverantwortung praktiziert und sie finden auch
heute noch auf vielen landwirtschaftlichen Betrieben Verwendung im Alltag. Um
diese Praxis zu dokumentieren, wurde in den Jahren 2018–2021 eine
ethnoveterinärmedizinische Studie in Bayern durchgeführt.
Material und Methoden Die Landwirt*innen wurden vor Ort interviewt und die
berichteten Rezepturen detailliert dokumentiert und wo möglich auch im Rahmen
der Interviews hergestellt. Dabei wurden die eingesetzten Pflanzenteile mittels
einer Laborfeinwaage nachgewogen, um letztlich für möglichst viele dieser
tradierten Anwendungen eine Tagesdosis je Tier bzw. die Konzentration im
Endprodukt errechnen zu können.
Ergebnisse Insgesamt wurden in den 77 Interviews 716 Rezepturen für 884
Anwendungen von den Landwirt*innen genannt. Zwei Drittel (69%) der genannten
Rezepturen bestanden aus einer oder mehreren Pflanzenarten und 31% befassten
sich mit anderen naturstofflichen Hausmitteln (Honig, Essig, etc.). Insgesamt
bevorzugten die befragten Landwirt*innen den Einsatz von Einzelpflanzen in ihren
Rezepturen. Die meisten Anwendungen wurden von den Teilnehmer*innen für Rinder
berichtet (77% aller Berichte).Mit Abstand am häufigsten wurden diese traditionellen Hausmittel in den
bayerischen Ställen zur Prophylaxe und Verabreichung bei gastrointestinalen
Erkrankungen und metabolischen Störungen eingesetzt. Außerdem ergab sich eine
hohe Übereinstimmung der eingesetzten Pflanzenarten mit beschriebenen
Anwendungen aus 2 historischen Fachbüchern. Die Studienteilnehmer*innen setzten
ihre Hausmittel mit hoher Zufriedenheit ein.
Schlussfolgerung und klinische Relevanz Dieses tradierte und seit
Generationen weitergegebene Wissen zu pflanzlichen und damit wirkstoffhaltigen
Zubereitungen kann auch für die tierärztliche Praxis eine gute Ausgangsbasis für
eine phytotherapeutische Ergänzung zur Behandlung von Nutztieren sein. Arznei-
und futtermittelrechtliche Rahmenbedingungen müssen hierbei selbstverständlich
eingehalten werden.
Wie die Blutprobe des Rettungsdienstes die Aufklärungsquote des plötzlichen, unerwarteten (Herz-)Todes bei jungen Menschen erhöhen kann – eine Übersicht
Authors: Tiesmeier Jens , Persson Jan , Hinkelbein Jochen , Greulich Andrea , Gärtner Anna , Milting Hendrik 
Published: May 2024

ZusammenfassungDie Hauptursachen für einen plötzlichen, nicht traumatischen und unerwarteten (Herz-)Tod (SCD) variieren mit dem Lebensalter. Dominiert, mit fließenden Übergängen, ab dem 35.–40. Lebensjahr die koronare Herzkrankheit, so sind dies bei jüngeren Menschen neben Myokarditiden und Intoxikationen auch arrhythmogene primäre Kardiomyopathien oder Ionenkanalerkrankungen. Ursächlich sind hier genetische Veränderungen, die durch eine molekulare Analyse in einer Vielzahl der Fälle nachgewiesen werden können. Bei außerklinischen Fällen eines SCD von jungen Menschen ist eine Blutprobe, entnommen durch den Rettungsdienst potenziell geeignet, Biomaterial für spätere Analysen sicherzustellen und die Aufklärungsquote genetischer Ursachen des außerklinischen SCD zu erhöhen. Dies erhält die Option für gezielte primär- oder sekundärprophylaktische Maßnahmen bei Überlebenden und/oder deren familiäres Umfeld.
Konsum von Partydrogen im Kölner Nachtleben – eine quantitative
Erhebung
Authors: Henning Denise , Pioch Fabian , Köhler Thorsten 
Published: May 2024

Zusammenfassung
Ziel der StudieDer Konsum psychoaktiver Substanzen in Deutschland ist
unter Partybesuchenden deutlich stärker verbreitet als in der
Allgemeinbevölkerung, jedoch lagen keine aktuellen Daten über die
Konsumhäufigkeit von Partydrogen im Kölner Nachtleben vor.
MethodikIm Rahmen dieser Studie wurde vor Ort auf Kölner Technopartys
(n=127) und online (n=726) bei Angehörigen der Technoszene der Drogenkonsum
mittels standardisierter Befragung untersucht.
Ergebnisse Beide Stichproben wiesen im Vergleich zur Allgemeinbevölkerung
stark erhöhte Konsumprävalenzen bei den legalen Substanzen Alkohol und Nikotin,
sowie den illegalen „Partydrogen“ MDMA, Kokain, Ketamin, Amphetaminen und
weiteren Substanzen auf. Riskanter polyvalenter Konsum ist in der Szene weit
verbreitet.
Schlussfolgerung Die Konsumprävalenzen von Partydrogen in der viertgrößten
deutschen Stadt zeigen eine starke Verbreitung der Substanzen. Daher sollte
verstärkt durch Präventionsfachstellen auf mögliche neurologische Folgen des
Konsums bis hin zu Todesfällen durch Überdosierungen hingewiesen werden. Um
Konsumierende vor verunreinigten und hochdosierten Substanzen zu schützen,
sollten spezifische Präventions- und Drugchecking-Angebote eingerichtet
werden.