bluuebunny/crossref_metadata_2025_split huggingface dataset:
The mouse as a suitable host for an isolate of Schistosoma haematobium from Niger
Authors: Imbert-Establet D. , Vera C. , Sellin B. , Jourdane J. 
Published: June 2009

ABSTRACTThe host-parasite relationships of a Schistosoma haematobium isolate, originating from Niger, and the white mouse are described. Swiss OF1 albino mice were exposed individually to 200 cercariae and worms were recovered 9, 12, 16 and 20 weeks post infection. The mean worm returns ranged between 10·54 and 13·5% and did not alter significantly between 9 and 20 weeks post infection. The sex ratio of worms was always in favour of males; from 7·9:1 at 9 weeks after infection it decreased regularly to 3·28:1 at 20 weeks. Male worms reached a mean length of 8·72 mm at 20 weeks. From the 12th week post infection, a high number of eggs was found in the liver and gut. At 20 weeks, eggs were also found in the bladder. Viable eggs and infective miracidia were obtained. The infection of Bulinus truncatus from Niger succeeded with a mean rate of 61% after the first passage through mice. The isolate of S. haematobium was maintained in the laboratory during 3 successive passages through mice. These entirely new results are very probably linked to genetic characteristics peculiar to the S. haematobium populations from Niger.
Life cycle of Gnathostoma nipponicum Yamaguti, 1941
Authors: Ando K. , Tokura H. , Matsuoka H. , Taylor D. , Chinzei Y. 
Published: June 2009

ABSTRACTThe life cycle of Gnathostoma nipponicum was examined by field survey and by experimental infection of animals with the larvae. Naturally infected larval G. nipponicum were found in loaches, catfish, and snakes. Experimentally, loaches, killifishes, frogs, salamanders, mice, and rats were successfully infected with the early third-stage larvae of G. nipponicum obtained from copepods (the first intermediate host), whereas snakes, quails, and weasels were not. Frogs, snakes, quails, and rats were experimentally infected with the advanced third-stage larvae (AdL3) from loaches. These results reveal that some species of fishes, amphibians and mammals can act as the second intermediate host and that some species of reptiles, birds and mammals can act as a paratenic host. The life cycle was completed in weasels, the definitive host, which were infected with AdL3 from loaches and started to evacuate eggs of G. nipponicum in faeces on days 65–90 postinfection.
Clinical manifestations of taeniasis in Taiwan aborigines
Authors: Fan P. C. , Chung W. C. , Lin C. Y. , Chan C. H. 
Published: June 2009

ABSTRACTFrom 1974 to 1989, a total of 24 500 aborigines at 67 villages in ten mountainous districts/towns in Taiwan were examined for the TaiwanTaeniainfection and 12% were found to be infected. In order to define the clinical manifestations of taeniasis caused by the TaiwanTaenia, 1661 aborigines in ten mountainous districts were surveyed. The overall clinical rate was 76%. The clinical rate was highest among Atayal aborigines (81%), followed by Bunun (66%) and Yami (61%) aborgines and lowest among Ami aborigines (40%). Among 1153 infected people, 10% had passed gravid segments in the faeces for less than 1 year, 24% for 1–3 years, 17% for 4–5 years. 23% for 6–10 years, 16% for 11–20 years, 7% for 21–30 years, and 3% over 30 years. Twenty-six occurrences of gastrointestinal and neurological symptoms were reported by 1258 infected persons. Passing proglottides in the faeces (95%) was the most frequent sign, followed by pruritis ani (77%), nausea (46%), abdominal pain (45%), dizziness (42%), increased appetite (30%), headache (26%). etc.
Protease resistant interleukin-3 stimulating components in excretory and secretory products from adult worms of Strongyloides ratti
Authors: Abe T. , Nawa Y. , Yoshimura K. 
Published: June 2009

ABSTRACTExcretory and secretory (ES) products collected from adult worms of Strongyloides ratti stimulated interleukin-3 (IL-3) production with mesenteric lymph node cells from infected C57BL/6 mice, hut not with normal mesenteric lymph node cells. The IL-3 stimulating components were not major IgG binding antigens. Activity of the IL-3 stimulating components was stable by treatment with protease, although reduced by heating in boiling water.
A morphological study of the effects of liposomized albendazole on the muscle phase of Trichinella spiralis in mice
Authors: Hrčkova G. , Velebny S. , Horák J. 
Published: June 2009

AbstractThe pathomorphological effects of daily and weekly doses of liposomized albendazole on Trichinella spiralis larvae have been examined and compared during the course of muscle infections in mice. Treatment with three and six daily doses of the drug given by intraperitoneal injection to mice resulted in marked pathological alterations to encapsulated larvae, mainly in the walls of the capsules. There was a 5% efficacy against T. spiralis in mice given three daily doses of albendazole, and 38% efficacy in mice given six daily doses of the drug. The same doses of drug, when administered weekly, did not reduce the numbers of parasite larvae, and the application of drug at 6-weekly intervals resulted in a 5% reduction in numbers.
Imported Opisthorchis viverrini and parasite infections from Thai labourers in Taiwan
Authors: Peng H. W. , Chao H. L. , Fan P. C. 
Published: June 2009

AbstractIn order to determine the prevalence of intestinal parasites among labourers from Thailand in Taiwan, the stool samples from 1364 Thai labourers were examined by the formalin-ether concentration method and 18·0% were found to be infected with one or more parasites. The infection rates of hookworm, Opisthorchis viverrini, Strongyloides stercoralis, Enterobius vermicularis, Ascaris lumbricoides, Hymenolepis nana, Capillaria philippinensis, Schistosoma japonicum, Echinostoma sp., Entamoeba coli, Giardia lamblia, and Entamoeba histolytica, were 7·6%, 7·0%, 3·2%, 0·5%, 0·3%, 0·1%, 0·1%, 0·l%,0·l%, 0·4%, 0·2%, and 0·1% respectively. The prevalence was highest among the 21–25 age group (24·8%). Among the 245 infected persons, 91% were infected with one parasite, 8% with two parasites and 1% with three parasites. The finding that parasitic infections are prevalent among Thai labourers demonstrates the need for control measures in foreign labourers in Taiwan and further studies to determine the susceptibility of O. viverrini to the snail hosts of Clonorchis sinensis.
Cytochemical observations on the nervous system of adultCorrigia vitta
Authors: Magee C. A. , Cahir M. , Halton D. W. , Johnston C. F. , Shaw C. 
Published: June 2009

AbstractAdultCorrigia vitta(Trematoda: Dicrocoelidea) inhabit the pancreatic duct of the fieldmouse,Apodemus sylvaticus, where, in numbers, they may occlude the duct lumen and prevent the flow of pancreatic secretions. Enzyme histochemical and immunocytochemical techniques, in conjunction with confocal scanning laser microscopy, have been used to examine the localization and distribution of cholinergic. serotoninergic (5-HT, serotonin) and peptidergic components of the nervous system of the adult worm. All three classes of neuronal mediator showed a common pattern of staining, occurring throughout the central and peripheral nervous systems. Of the four peptide immunoreactivities (IR) demonstrated (pancreatic polypeptide (PP), peptide YY (PYY), substance P (SP), FMRFamide), PP-IR was the most predominant, occurring not only within the central ganglia and longitudinal nerve cords, but also in subtegumental plexuses and in fibres associated with the egg-forming apparatus. PYY and FMRFamide IRs were evident throughout the central and peripheral nervous systems; FMRFamide immunostaining, in particular, highlighted innervation of the ootype and immunoreactive cell bodies around the Mehlis' gland. Both SP- and 5-HT-IRs were restricted to the cerebral ganglia, ventral nerve cords and associated cell bodies. The distribution pattems of these peptides and 5-HT within the nervous system ofC. vittasuggest they are likely to function as neuronal mediators. PP, PYY and FMRFamide may also serve in regulating egg production.
Cryopreservation of Angiostrongylus cantonensis third-stage larvae
Authors: Mahajan R. K. , Renapurkar D. M. 
Published: June 2009

AbstractAn optimum protocol for the cryopreservation of third-stage larvae (L3) of Angiostrongylus cantonensis was determined by: (i) use of different dimethyl sulphoxide (DMSO) concentrations; (ii) use of different FBS concentrations in RPMI-1640 medium; and (iii) variation in the thawing temperature. Studies showed that with Concentrations of DMSO at 4·8% and FBS at 40% and a thawing temperature of 43°C, L3 survival assessed by motility was highest at 56%. The infectivity of the cryopreserved L3 of A. cantonensis was 20% compared to controls obtained from slugs.
Levels of ecdysteroid-like material in adults of Nippostrongylus brasiliensis during the intestinal phase
Authors: Nembo B. , Duie P. , Garcia M. , Breton P. , Gayral P. , Porcheron P. , Goudey-Perriere F. 
Published: June 2009

AbstractTotal ecdysteroid-like immunoreactive material was assayed and quantified in adults of the parasitic nematode N. brasiliensis during the intestinal phase in the rat in order to detect possible physiological fluctuations in titre. Worms of the same sex isolated from one rat were pooled in order to quantify ecdysteroids using enzyme immunoassay (EIA). The concentration of ecdysteroids fluctuated during adult life according to the sex and the age of the parasite. Important differences of levels of ecdysteroid-like compounds between the two sexes of parasites were noted particularly at 128 and 168 h post-infection. The peak at 128 h, present in female, but not in male worms, corresponds to the time of egg-laying. Following HPLC-EIA analysis, the presence of ecdysone, 20-hydroxyecdysone and apolar ecdysteroid-like material was ascertained during this peak. The physiological role and the possible origin of ecdysteroids in this nematode are discussed.
Partial characterization and time course analysis of Hymenolepis diminuta coproantigens
Authors: Allan J.C. , Craig P.S. 
Published: June 2009

AbstractAn analysis of Hymenolepis diminuta specific antigens in infected rat faeces was carried out. Using a capture type antibody sandwich ELISA assay based on a hyperimmune rabbit anti-worm somatic antisera it was demonstrated that, although antigen was present in faeces before patency, the onset of egg production led to a sharp increase in the levels of parasite antigen in the faeces. Levels of antigen in host faeces were independent of worm burden. Parasite eggs did not contribute significantly to faecal antigen levels. Western blot analysis indicated a number of highly specific antigens at around Mr 69,000, Mr 37,000, Mr 50,000 and Mr 27,000 with a low molecular weight smear at between Mr 30,000 and Mr 34,000 present in the faeces of H. diminuta infected rats. Some cross reaction occurred with an antigen of around Mr 66,000 in the faeces of non H. diminuta infected rodents. Antibody activity against this antigen was removed by affinity adsorption of the antibody solution against normal rat faeces.
Life history of the nematode Rhabdochona hellichi, a parasite of the barbel in the Jihlava River, Czech Republic
Authors: Moravec F. , Scholz T. 
Published: June 2009

AbstractFrom April 1992 until June 1993, the seasonal dynamics of Rhabdochona hellichi (Šrámek 1901) in its definitive host, the barbel (Barbus barbus), was studied in the Jihlava River (the Danube basin), Czech Republic. The parasite occurred in barbel throughout the year (overall prevalence 93% and intensity of infection 1–1384 [mean 78] nematodes per fish), but there were distinct seasonal fluctuations in prevalence and mean intensity values associated with the parasite's seasonal cycle of maturation. Prevalence was very high (usually 100%) during most months of the year, while lowest values were recorded in August and October. The highest values of mean intensity occurred in April. Both prevalence and mean intensity values increased with the body length of the hosts. The parasite exhibited a distinct annual cycle of maturation with egg-producing females present only fmm June until August, whereas larvae occurred in barbel mostly from September until April of the next year. Trichopteran (caddis-fly) larvae of the genus Hydropsyche (H. angustipennis and H. pellucidula) were found as natural intermediate hosts of R. hellichi (prevalence 4%, intensity 1–10 nematode larvae). The strictly seasonal maturation of this nematode may be associated with the temperature regime in the locality and seasonal cycles of maturation of its intermediate hosts.
Trichrome staining ofGyrodactylussclerites and soft tissues following fixation in ammonium picrate-glycerin, including an improved rendition of the haptoral bars ofG. turnbulli
Authors: Richards G.R. , Chubb J.C. 
Published: June 2009

AbstractA simple technique using modified Mallory stain in the transferral ofGyrodactylusspecimens from ammonium picrate-glycerin to a permanent mountant is described. Hamuli, their connecting bars and the penis sclerites are well defined by the technique as are muscles and tendons, cell nuclei, tegument and gland cells. As well as being useful in the study of general anatomy, the technique enhances the observation of the taxonomically important ventral and dorsal bars. In order to show this, improved illustrations of the dorsal and ventral bars ofG. turnbulliare given along with explicit demonstrations of differences in morphology of the ventral bars ofG. bullatarudisandG. rasini- two easily confused species.
A bolus for the administration to cattle of metacercariae of the liver fluke Fasciola hepatica
Authors: Andrews S.J. , McGonigle S. , Smith A.M. , Dalton J.P. , Clery D. , Mulcahy G. 
Published: June 2009

AbstractA gelatin-agar bolus, designed and developed for the administration of metacercariae of the liver fluke Fasciola hepatica, was evaluated in adult Holstein Friesian cattle. The metacercariae, enclosed within a gelatin capsule, were placed inside the bolus and delivered to each animal using an oesophageal balling gun. At slaughter, 13 weeks after challenge, an average of 25% of the challenge dose was recovered from each liver. This percentage recovery is similar to that obtained with other known methods. The new bolus, however, offers improved handling qualities. In addition, the bolus also has potential for improving a number of other techniques including those for the administration of other parasites, compounds or chemotherapeutic agents.
Cytochemical localization of cytochrome c oxidase activity in mitochondria in the tegument and tegumental and parenchymal cells of the trematodes Echinostoma trivolvis, Zygocotyle lunata, Schistosoma mansoni, Fasciola gigantica and Paragonimus ohirai
Authors: Fujino T. , Fried B. , Takamiya S. 
Published: June 2009

AbstractCytochrome c oxidase in the mitochondria of the tegument and tegumental and parenchymal cells was examined cytochemically in Echinostoma trivolvis, Zygocotyle lunata, Schistosoma mansoni, Fasciola gigantica and Paragonimus ohirai, trematodes that inhabit different sites in their vertebrate hosts. Clear differences in enzyme activity occurred in the mitochondria of these species, probably reflecting the different energy metabolisms of these worms. Marked aerobic metabolism occurred in S. mansoni and P. ohirai adults that inhabit the host mesenteric veins and the lungs, respectively. The tegument and parenchymal cells of S. mansoni possess relatively few, small mitochondria with tubular cristae which are heavily reactive for cytochrome c oxidase. In P. ohirai, the activity for cytochrome c oxidase in tegumental mitochondria increased gradually from juveniles to adults, reflecting that the respiratory activity increased with growth and the aerobic metabolism is activated when the worms reach the lung. P. ohirai juveniles and adults had two types of mitochondria with different shapes and enzyme activities that were located in two different types of parenchymal cells. The intestinal species, E. trivolvis had mitochondria in the basal aspect of the tegument, and some variations in enzyme activity of their mitochondria in the tegumental and parenchymal cells were observed, suggesting that they possess both aerobic and anaerobic metabolic systems. Z. lunata that live in rodent caeca are devoid of mitochondria in the tegument and have many characteristic mitochondria with undeveloped cristae in the parenchymal cells. Mitochondria of F. gigantica showed weak or no activity for cytochrome c oxidase, suggesting that the worm is well-adapted to an anaerobic environment in the host bile duct.
Note on the first occurrence of larval Echinococcus multilocularis in Clethrionomys rex in Hokkaido, Japan
Authors: Takahashi K. , Nakata K. 
Published: June 2009

AbstractLarval Echinococcus multilocularis was found in Clethrionomys rex in Hokkaido, Japan. C. rex is a new host record for E. multilocularis. The cysts were filled with numerous protoscoleces suggesting a potential role of this vole in a natural cycle of transmission.
Genetic variation in the triploids of Japanese Fasciola species, and relationships with other species in the genus
Authors: Agatsuma T. , Terasaki K. , Yang L. , Blair D. 
Published: June 2009

AbstractTwelve enzymes (encoded by 14 loci) in liver flukes of Fasciola species originating from Japan (parthenogenetic triploids), Korea (parthenogenetic diploids), the United States of America (USA) and Australia (all sexual diploids) were analysed using starch gel electrophoresis. Variation in electrophoretic patterns between samples was detected at five enzyme loci (Ak, Got, Gpi, 6-Pgd and Pgm-2). Japanese worms (31, of which six were established as uniparental laboratory strains), which reproduce by parthenogenesis, exhibited three different isozyme patterns. This indicates that triploidy has arisen more than once in Japanese flukes. Japanese Fasciola sp. can be separated into three types on morphological grounds. For the six laboratory strains of Japanese worms, the parental morphological type was known. Each of the three isozyme patterns observed was restricted to one morphological type. Most alleles detected in the Japanese triploids were also found in diploid worms from the other countries: the only alleles not represented elsewhere were four at the Got locus and two at the Pgm locus. Flukes from a laboratory strain derived from a single Korean diploid worm resembled the Japanese worms in genotype more closely than did American (seven uniparental laboratory strains) or Australian (30 worms) specimens. Worms from the last two countries were closely related.
Larval development of ovineNeostrongylus linearisin four experimentally infected mollusc species
Authors: Manga-González M.Y. , Morrondo-Pelayo M.P. 
Published: June 2009

AbstractDevelopment ofNeostrongylus linearislarvae was studied in the snail intermediate hostsOestophora(Oestophora)barbula, Oestophorella buvinieri, Cepaea nemoralisandHelix(Cryptomphalus)aspersa. The molluscs of each species, all adults, were divided into groups of 40 for infection purposes. The infection doses for the first two snail species were 90 and 50 first stage larvae (L1) ofN. linearis, respectively. ForC. nemoralistwo batches were tested: one with 200 L1 kept at 19°C and the other with 250 L1 at a temperature of 21°C. The same was done withHelix(C.)aspersawith 200 L1 at a temperature of 21°C, on the one hand and 300 L1 at 24°C on the other. One or two molluscs of each species were killed in series from the 6th day post-infection (p.i.) until the 44th. Percentage values for total larvae (1, 2 and 3) and L3 were higher with the lower dose forC. nemoralis, whilst the same was true with the higher dose inHelix(C.)aspersa. In both cases, the higher temperature appeared to contribute to cycle acceleration. Using one way analysis of variance, statistically significant differences were detected between the species of molluscs tested concerning percentages of L1 which penetrated, total larvae and L3. According to our results, the decreasing order of susceptibility of these species of molluscs as experimental intermediate hosts ofN. linearisis:O. buvinieri,Oestophora(O.)barbula,C. nemoralisandHelix(C.)aspersa. It is the first time thatOestophora(O.)barbulaandOestophorella buvinierihave been named as experimental intermediate hosts ofN. linearis.
Anisakis simplex: stage-specific antigens recognized by mice
Authors: Iglesias R. , Leiro J. , Ubeira F.M. , Santamarina M.T. , Sanmartín M.L. 
Published: June 2009

AbstractTo investigate antigenic differences between the developmental stages ofAnisakis simplex, somatic, excretion–secretion and detergent-solubilized surface antigen preparations of third- and fourth-stageA. simplexlarvae, and a somatic antigen preparation of adults, were characterized by SDS–PAGE and immunoblotting using mouse immune serum containing antibodies against the two larval stages. Excretion–secretion and surface antigen preparations from thirdstage larvae behaved very differently from those of fourth-stage larvae, in both SDS-PAGE and immunoblotting; this suggests that certain excretion–secretion and surface antigens are highly stage-specific. By contrast, somatic components of third- and fourth-stage larvae were found to have very similar banding patterns, suggesting conservation of these components during the development of the parasite in mice. The SDS-PAGE and immunoblotting results for adult somatic components seem to support this hypothesis
Failure of experimental infection of baboons (Papio hamadryas) with the eggs of Asian Taenia
Authors: Fall E.H. , Geerts S. , Kumar V. , Vervoort T. , Deken R. De , Eom K. S. 
Published: June 2009

AbstractThe infectivity of the eggs of Asian Taenia sp. for humans is not known. Using baboons (Papio hamadryas) as a model to extrapolate the findings, three animals were exposed per os with 1000, 10,000 and 50,000 infective eggs of theAsian Taenia sp. The serological, biochemical, haematological and parasitological (based on necropsy) results show that baboons are refractory to the infection. It is concluded that the Asian taeniid eggs may fail to develop in man.
The influence of neuroactive pesticides on the behaviour of entomopathogenic nematodes
Authors: Patel M.N. , Wright D.J. 
Published: June 2009

AbstractThe behaviour and infectivity of the infective juveniles (IJs) of two species of entomopathogenic nematodes, Steinernema carpocapsae Weiser and Steinernema feltiae Filipjev (Rhabditida: Steinernematidae), were determined after exposure to two neuroactive pesticides, oxamyl and fenamiphos, widely used against plant parasitic nematodes. Oxamyl (50 μg ml−1) initially stimulated non-sinusoidal movement in S. feltiae but had no appreciable effect on IJs of S. carpocapsae, while fenamiphos (10 and 50 μg ml−1) increased and sustained non-sinusoidal movement in both species over an observation period of 48 h. The two pesticides reduced the infectivity of both nematode species against late instar larvae of the wax moth, Galleria mellonella L. in a sand-tube bioassay when compared with controls. Infective juveniles of S. feltiae showed limited body waving behaviour on the sand surface whereas S. carpocapsae showed the full range of nictation movements, including body waving, stationary straight postures and leaping. Oxamyl had no significant (P>0.05) effect on the number of IJs of S. carpocapsae which nictated except when the IJs were pre-incubated with this compound for 24 h (P<0.05). In contrast, fenamiphos completely suppressed all nictation and body waving behaviour in both species. Nictating IJs of S. carpocapsae exposed to late instar G. mellonella for 12 h in a filter-paper bioassay were found to be more infective compared with non-nictating IJs. Treating IJs of S. carpocapsae with oxamyl (50 μg ml−1) increased the infectivity of non-nictating IJs compared with controls but reduced infectivity of nictating IJs when exposed to G. mellonella for 12 h. The work is discussed in relation to proposals to use these or other pesticides to enhance the activity and infectivity of IJs of entomopathogenic nematodes.