bluuebunny/crossref_metadata_2025_split huggingface dataset:
Management of Colorectal Neoplasia in IBD Patients: Current Practice and Future Perspectives
Authors: Derks Monica E W , te Groen Maarten , van Lierop Lisa M A , Murthy Sanjay , Rubin David T , Bessissow Talat , Nagtegaal Iris D , Bemelman Willem A , Derikx Lauranne A A P , Hoentjen Frank 
Published: May 2024

Abstract
Inflammatory bowel disease [IBD] patients are at increased risk of developing colorectal neoplasia [CRN]. In this review, we aim to provide an up-to-date overview and future perspectives on CRN management in IBD. Advances in endoscopic surveillance and resection techniques have resulted in a shift towards endoscopic management of neoplastic lesions in place of surgery. Endoscopic treatment is recommended for all CRN if complete resection is feasible. Standard [cold snare] polypectomy, endoscopic mucosal resection and endoscopic submucosal dissection should be performed depending on lesion complexity [size, delineation, morphology, surface architecture, submucosal fibrosis/invasion] to maximise the likelihood of complete resection. If complete resection is not feasible, surgical treatment options should be discussed by a multidisciplinary team. Whereas [sub]total and proctocolectomy play an important role in management of endoscopically unresectable CRN, partial colectomy may be considered in a subgroup of patients in endoscopic remission with limited disease extent without other CRN risk factors. High synchronous and metachronous CRN rates warrant careful mucosal visualisation with shortened intervals for at least 5 years after treatment of CRN.
Insertion–Deletion Events Are Depleted in Protein Regions with Predicted Secondary Structure
Authors: Yang Yi , Braga Matthew V , Dean Matthew D 
Published: May 2024

Abstract
A fundamental goal in evolutionary biology and population genetics is to understand how selection shapes the fate of new mutations. Here, we test the null hypothesis that insertion–deletion (indel) events in protein-coding regions occur randomly with respect to secondary structures. We identified indels across 11,444 sequence alignments in mouse, rat, human, chimp, and dog genomes and then quantified their overlap with four different types of secondary structure—alpha helices, beta strands, protein bends, and protein turns—predicted by deep-learning methods of AlphaFold2. Indels overlapped secondary structures 54% as much as expected and were especially underrepresented over beta strands, which tend to form internal, stable regions of proteins. In contrast, indels were enriched by 155% over regions without any predicted secondary structures. These skews were stronger in the rodent lineages compared to the primate lineages, consistent with population genetic theory predicting that natural selection will be more efficient in species with larger effective population sizes. Nonsynonymous substitutions were also less common in regions of protein secondary structure, although not as strongly reduced as in indels. In a complementary analysis of thousands of human genomes, we showed that indels overlapping secondary structure segregated at significantly lower frequency than indels outside of secondary structure. Taken together, our study shows that indels are selected against if they overlap secondary structure, presumably because they disrupt the tertiary structure and function of a protein.
Funding of evidence included within public comments submitted to inform Medicare national coverage determinations
Authors: Lu Angela , Ji Robin Z , Magee Marley P D , Ross Joseph S , Ramachandran Reshma , Redberg Rita F , Dhruva Sanket S 
Published: May 2024

Abstract
The Centers for Medicare & Medicaid Services (CMS) relies on public comments submitted in response to proposed national coverage determinations to assist the agency in determining the coverage of items and services for Medicare beneficiaries. In a cross-sectional study, we characterized the cited evidence and what funding supported the cited evidence submitted in public comments to CMS for all therapeutic medical device national coverage determinations finalized between June 2019 and June 2022. Of 681 public comments, 159 (23%) cited at least 1 identifiable published scientific journal article. Within these 159 public comments, 198 unique articles were cited, 170 (86%) of which included funding statements or author disclosures. Among these, 96 (56%) disclosed funding from manufacturers that would benefit from Medicare coverage and/or were written by author(s) who received funding from these manufacturers. In summary, most public commenters for national coverage determinations did not cite published scientific journal articles to support their positions. Among those who did, more than half of articles were directly funded by manufacturers that would benefit from coverage. Greater funding of independent, non–industry-supported research may help provide unbiased evaluations of benefits and harms to support Medicare coverage decisions.
Heated tobacco product marketing: a mixed-methods study examining exposure and perceptions among US and Israeli adults
Authors: Cui Yuxian , Bar-Zeev Yael , Levine Hagai , LoParco Cassidy R , Duan Zongshuan , Wang Yan , Abroms Lorien C , Khayat Amal , Berg Carla J 
Published: May 2024

Abstract
The marketing of heated tobacco products (HTPs), like IQOS, influences consumers’ perceptions. This mixed-methods study analyzed (i) survey data (2021) of 2222 US and Israeli adults comparing perceptions of 7 IQOS attributes (design, technology, colors, customization, flavors, cost and maintenance) and 10 marketing messages (e.g. ‘Go smoke-free…’) across tobacco use subgroups and (ii) qualitative interviews (n = 84) regarding IQOS perceptions. In initial bivariate analyses, those never using HTPs (86.2%) reported the least overall appeal; those currently using HTPs (7.7%) reported the greatest appeal. Notably, almost all (94.8%) currently using HTPs also currently used cigarettes (82.0%) and/or e-cigarettes (64.0%). Thus, multivariable linear regression accounted for current cigarette/e-cigarette use subgroup and HTP use separately; compared to neither cigarette/e-cigarette use (62.8%), cigarette/no e-cigarette use (17.1%) and e-cigarette/no cigarette use (6.5%), those with dual use (13.5%) indicated greater overall IQOS appeal (per composite index score); current HTP use was not associated. Qualitative data indicated varied perceptions regarding advantages (e.g. harm, addiction and complexity) of IQOS versus cigarettes and e-cigarettes, and perceived target markets included young people, those looking for cigarette alternatives and females. Given the perceived target markets and particular appeal to dual cigarette/e-cigarette use groups, IQOS marketing and population impact warrant ongoing monitoring to inform regulation.
Participation in global value chains and M&A flows
Authors: Ciani Andrea , Gregori Wildmer Daniel 
Published: May 2024

Abstract
This study investigates whether companies operating in industrial sectors characterized by a higher participation in global value chains (GVC) are more likely to be involved in cross-border mergers and acquisitions (M&A). We focus on firms acquired in the EU27 during the period 2008–2020 implementing a gravity model. Results show that cross-border investments are indeed associated with sectoral GVC participation, in particular the dependence on intermediates supplied by other countries (i.e., backward GVC participation) of the target country-sector, which is positively correlated with M&A flows. This evidence is confirmed when the acquired firm operates in high-tech sectors, and when the investor is based in OECD countries. In addition, we find that companies from non-OECD countries supplying inputs to other countries (i.e., higher forward GVC participation) are more likely to pursue a cross-border acquisition.
Institutional investors and dividend payments: evidence in the oil industry
Authors: Machado João Victor , Sarti Fernando , Silveira Rodrigo Lanna Franco da 
Published: May 2024

Abstract
The debate over the impacts of shareholder value orientation on corporate management has been more intense with the increasing participation of institutional investors in companies’ ownership structures. In this context, the purpose of this study is to evaluate the influence of institutional investors’ shareholding on the payment of dividends in the oil industry. A regression model was used, estimated with the Generalized Method of Moments. The results indicated that the distribution of dividends is related to the profitability and the leverage of the companies, in addition to the history of distribution to shareholders. In general, the presence of institutional investors did not influence the dividend distribution. However, we observed a large participation of these investors in the ownership structure of companies in the oil and gas sector—the average control of these agents was around 25% in the companies of the sample. This study contributes to the literature regarding the influence of institutional investors on the corporate decisions of nonfinancial companies, being original in the context of the oil industry.
New Combinatorial Identity for the Set of Partitions and Limit Theorems in Finite Free Probability Theory
Authors: Arizmendi Octavio , Fujie Katsunori , Ueda Yuki 
Published: May 2024

Abstract
We provide a refined combinatorial identity for the set of partitions of $\{1,\dots , n\}$, which plays an important role in investigating several limit theorems related to finite free convolutions. Firstly, we present the finite free analogue of Sakuma and Yoshida’s limit theorem. That is, we provide the limit of $\{D_{1/m}((p_{d}^{\boxtimes _{d}m})^{\boxplus _{d}m})\}_{m\in{\mathbb{N}}}$ as $m\rightarrow \infty $ in two cases: (i) $m/d\rightarrow t$ for some $t&gt;0$ or (ii) $m/d\rightarrow 0$. The second application presents a central limit theorem for finite free multiplicative convolution. We establish a connection between this theorem and the multiplicative free semicircular distributions through combinatorial identities. Our last result gives alternative proofs for Kabluchko’s limit theorems concerning the unitary Hermite and the Laguerre polynomials.
Integral Models of Moduli Spaces of Shtukas with Deep Bruhat-Tits Level Structures
Authors: Bieker Patrick 
Published: May 2024

Abstract
We construct integral models for moduli spaces of shtukas with deeper Bruhat-Tits level structures. We embed the moduli space of global shtukas for a deeper Bruhat-Tits group scheme into the limit of the moduli spaces of shtukas for all associated parahoric group schemes. Its schematic image defines an integral model of the moduli space of shtukas with deeper Bruhat-Tits level with favourable properties. They admit proper, surjective and generically étale level maps as well as a natural Newton stratification. In the Drinfeld case, this general construction of integral models recovers the moduli space of Drinfeld shtukas with Drinfeld level structures.
Humoral responses are enhanced by facilitating B cell viability by Fcrl5 overexpression in B cells
Authors: Ono Chisato , Kochi Yuta , Baba Yoshihiro , Tanaka Shinya 
Published: May 2024

Abstract
B cell initial activity is regulated through a balance of activation and suppression mediated by regulatory molecules expressed in B cells; however, the molecular mechanisms underlying this process remain incompletely understood. In this study, we investigated the function of the Fc receptor-like (Fcrl) family molecule Fcrl5, which is constitutively expressed in naive B cells, in humoral immune responses. Our study demonstrated that B cell-specific overexpression of Fcrl5 enhanced antibody (Ab) production in both T cell-independent type 1 (TI1) and T cell-dependent (TD) responses. Additionally, it promoted effector B cell formation under competitive conditions in TD responses. Mechanistically, in vitro ligation of Fcrl5 by agonistic Abs reduced cell death and enhanced proliferation in lipopolysaccharide-stimulated B cells. In the presence of anti-CD40 Abs and IL-5, the Fcrl5 ligation not only suppressed cell death but also enhanced differentiation into plasma cells. These findings reveal a novel role of Fcrl5 in promoting humoral immune responses by enhancing B cell viability and plasma cell differentiation.
Eliciting the barriers and enablers towards anaesthetists giving penicillin-based antibiotic prophylaxis to low-risk patients who have had their penicillin allergy label removed as part of a preoperative delabelling process
Authors: Roberts Neil , Fontaine Libby , Sandoe Jonathan , Tonkin-Crine Sarah , Powell Neil 
Published: May 2024

Abstract

Background
Some penicillin allergy labels can be removed by non-allergy specialists by direct oral challenge, but there is reluctance amongst anaesthetists to give penicillin to these patients. We aimed to assess anaesthetist beliefs about giving penicillin to patients delabelled by direct oral challenge.

Methods
A survey, developed using the Theoretical Domains Framework, was circulated to anaesthetists within a regional research network in England. Domains were rated using 5-point Likert scales. Overall and group medians were used to dichotomize domains rated by group into ‘relatively important/unimportant’ and ‘relative enabler/barrier’.

Results
We received 257 responses from six hospitals (response rate 49.7%). Seven domains were rated as important for all stakeholder groups and hospitals: Knowledge, Skills, Belief in Capabilities, Belief in Consequences, Memory/Attention/Decisions, Environmental Context and Resources, and Emotions. Social and Professional Role was also important to all respondents except those in one hospital. Intentions and Optimism were rated as important for some groups/hospitals and unimportant for others. All four other domains were rated as unimportant for all groups/hospitals. All domains rated as important were enablers for all groups/hospitals, with the exception of Memory/Attention/Decisions and Emotions, which were rated as discordant barriers/enablers between groups. This means they were acting as a barrier for some staff groups/hospitals and an enabler for others. Barrier domains (Reinforcement, Goals, Social Influences, Behavioural Regulation) were all rated unimportant.

Conclusions
Behavioural influences on giving penicillin prophylaxis to a delabelled patient are complex and nuanced. These findings could inform targeted interventions, both across and within hospitals and staff groups.
Emergence of an IncX3 plasmid co-harbouring the carbapenemase genes blaNDM-5 and blaOXA-181
Authors: Zuo Hui , Sugawara Yo , Kondo Kohei , Kayama Shizuo , Kawakami Sayoko , Uechi Kohei , Nakano Ami , Yahara Koji , Sugai Motoyuki 
Published: May 2024

Abstract

Background
The spread of transmissible plasmids with carbapenemase genes has contributed to a global increase in carbapenemase-producing Enterobacterales over the past two decades, with blaNDM and blaOXA among the most prevalent carbapenemase genes.

Objectives
To characterize an Escherichia coli isolate co-carrying blaNDM-5 and blaOXA-181 (JBEHAAB-19-0176) that was isolated in the Japan Antimicrobial Resistant Bacterial Surveillance in 2019–20, and to evaluate the functional advantage of carrying both genes as opposed to only one.

Methods
The whole-genome sequence of the isolate was determined using long- and short-read sequencing. Growth assay and co-culture experiments were performed for phenotypic characterization in the presence of different β-lactam antibiotics.

Results
WGS analysis showed that blaNDM-5 and blaOXA-181 were carried by the same IncX3 plasmid, pJBEHAAB-19-0176_NDM-OXA. Genetic characterization of the plasmid suggested that the plasmid emerged through the formation of a co-integrate and resolution of two typical IncX3 plasmids harbouring blaNDM-5 and blaOXA-181, which involved two recombination events at the IS3000 and IS26 sequences. When cultured in the presence of piperacillin or cefpodoxime, the growth rate of the transformant co-harbouring blaNDM-5 and blaOXA-181 was significantly higher than the transformant with only blaNDM-5. Furthermore, in co-culture where the two blaNDM-5-harbouring transformants were allowed to compete directly, the strain additionally harbouring blaOXA-181 showed a marked growth advantage.

Conclusions
The additional carriage of blaOXA-181 confers a selective advantage to bacteria in the presence of piperacillin and cefpodoxime. These findings may explain the current epidemiology of carbapenemase-producing Enterobacterales, in which bacteria carrying both blaNDM-5 and blaOXA-48-like genes have emerged independently worldwide.
The lack of EphB3 receptor prevents bone loss in mouse models of osteoporosis
Authors: Rodríguez-Sosa Mariano R , del Castillo Luis M , Belarra Adrián , Zapata Agustín G , Alfaro David 
Published: May 2024

Abstract
Bone homeostasis is a complex process in which some Eph kinase receptors and their ephrin ligands appear to be involved. In the present study, we address this issue by examining, both in vitro and in vivo, the role of EphB2 and EphB3 in mesenchymal stromal/stem cell (MSC) differentiation into bone tissue. This was first evaluated by quantitative reverse transcription PCR (RT-qPCR) and histological staining in MSCs cultured in specific mediums revealing that although EphB2−/− MSCs mainly expressed pro-adipogenic transcription factors, EphB3−/− MSCs showed abundant osteogenic transcripts, such as Runx2, Msx2, and Sp7. To clarify the underlying molecular mechanisms, we found that the lack of EphB3 signaling alters the genetic profile of differentiating MSCs, reducing the expression of many inhibitory molecules and antagonists of the BMP signaling pathway, and increasing Bmp7 expression, a robust bone inductor. Then, to confirm the osteogenic role of EphB3 in vivo, we studied the condition of 2 mouse models of induced bone loss (ovariectomy or long-term glucocorticoid treatment). Interestingly, in both models, both WT and EphB2−/− mice equally developed the disease but EphB3−/− mice did not exhibit the typical bone loss, nor an increase in urine Ca2+ or blood serum CTX-1. This phenotype in EphB3-KO mice could be due to their significantly higher proportions of osteoprogenitor cells and preosteoblasts, and their lower number of osteoclasts, as compared with WT and EphB2-KO mice. Thus, we conclude that EphB3 acts as a negative regulator of the osteogenic differentiation, and its absence prevents bone loss in mice subjected to ovariectomy or dexamethasone treatment.
Coevolution of non-homologous end joining efficiency and encephalization
Authors: Udroiu Ion , Sgura Antonella 
Published: May 2024

Abstract
Double-strand breaks (DSB), the most difficult to repair DNA damage, are mainly repaired by non-homologous end-joining (NHEJ) or homologous recombination (HR). Previous studies seem to indicate that primates, and particularly humans, have a better NHEJ system. A distinctive feature of the primate lineage (beside longevity) is encephalization, i.e., the expansion of the brain relative to body mass (BM). Using existing transcriptome data from 34 mammalian species, we investigated the possible correlations between the expression of genes involved in NHEJ and encephalization, BM, and longevity. The same was done also for genes involved in the HR pathway. We found that, while HR gene expression is better correlated with longevity, NHEJ gene expression is strongly (and better) correlated with encephalization. Since the brain is composed of postmitotic cells, DSB repair should be mainly performed by NHEJ in this organ. Therefore, we interpret the correlation we found as an indication that NHEJ efficiency coevolved with encephalization.
Sperm competition favours intermediate sperm size in a hermaphrodite
Authors: Santhosh Santhosh , Ebert Dieter , Janicke Tim 
Published: May 2024

Abstract
Sperm competition is a potent mechanism of postcopulatory sexual selection that has been found to shape reproductive morphologies and behaviours in promiscuous animals. Especially sperm size has been argued to evolve in response to sperm competition through its effect on sperm longevity, sperm motility, the ability to displace competing sperm, and ultimately fertilization success. Additionally, sperm size has been observed to co-evolve with female reproductive morphology. Theoretical work predicts that sperm competition may select for longer sperm but may also favour shorter sperm if sperm size trades-off with number. In this study, we studied the relationship between sperm size and postmating success in the free-living flatworm, Macrostomum lignano. Specifically, we used inbred isolines of M. lignano that varied in sperm size to investigate how sperm size translated into the ability of worms to transfer and deposit sperm in a mating partner. Our results revealed a hump-shaped relationship with individuals producing sperm of intermediate size having the highest sperm competitiveness. This finding broadens our understanding of the evolution of sperm morphology by providing empirical support for stabilizing selection on sperm size under sperm competition.
Evaluation of the most popular annual flowers sold in the United States and Europe indicates low visitation rates by pollinators and large variation among cultivars
Authors: Smitley David , Oneil Colin , Hotchkiss Erica , Runkle Erik , Studyvin Jared 
Published: May 2024

Abstract
To better understand how frequently pollinators visit the most popular annuals and the variation among cultivars, we evaluated 3–6 cultivars, each of petunia, impatiens, begonia, geranium, pansy, and New Guinea impatiens. These 6 annuals account for 46.6% of all garden center annual flower sales in the United States. Flower visits by honey bees, bumble bees, syrphids, other Diptera and other Hymenoptera, combined, varied 3 to 10-fold among cultivars within each of the 6 popular annuals. Begonia and impatiens were visited more frequently by pollinators than pansy, petunia, NG impatiens, and geranium. The 4 most visited cultivars, begonia ‘Cocktail Brandy’, begonia ‘Ambassador Rose Blush’, impatiens ‘Accent Coral’, and impatiens ‘Super Elfin XP White’ attracted as many pollinators as a benchmark annual, marigold ‘Alumia Vanilla Cream’, considered as moderately attractive to pollinators. Some conclusions from this research may be helpful for homeowners, landscapers, growers, and breeders. First, the most popular annual flowers are not a good choice for the purpose of attracting and supporting pollinators. However, the large variation among cultivars provides an opportunity to select cultivars that are more attractive to pollinators, particularly for begonia and impatiens. If the most pollinator-visited cultivars of begonia and impatiens are labeled and promoted as such, it would be beneficial to pollinators in urban and suburban landscapes in the USA and Europe, where they comprise 10%–20% of all annual flowers purchased from garden centers.
The Effect of Ophthalmic Antibiotics on Clinical Outcomes and Transmissibility of Conjunctivitis Associated with Haemophilus influenzae versus Other Pathogens: Secondary Analysis of a Randomized Controlled Trial
Authors: Thomas Elizabeth T , Perera-Salazar Rafael , Frost Holly M 
Published: May 2024

Abstract
Transmission rates among children with conjunctivitis were low and antibiotic use was not associated with reduced transmission. Policies recommending exclusion from daycare and school for conjunctivitis should be scrutinized as they may not reduce transmission and may increase unnecessary antibiotic use.