bluuebunny/crossref_metadata_2025_split huggingface dataset:
Natural Infection of Nude Mice with Low‐virulent Mouse Coronavirus
Authors: Hirano N. , Ono K. 
Published: May 2010

SummaryThe outbreak of wasting disease of nude mice occurred in the laboratory colony of a Pharmaceutical Company. The viruses producing cytopathic effect with syncytium formation were isolated from the wasted nude mice by DBT cells, and were identified as mouse coronavirus by direct immunofluorescence. The nude mouse colony was closed and all the nude mice (about 500) were killed by the reason of disease control. At autopsy about 60 % of nude mice showed necrotic hepatitis. By the virus isolation to see the source of contamination, viruses were isolated from the feces of apparently healthy mice of ICR, CDF1, DBA/2 and C3H, and from human cancer cell line stocked in liquid nitrogen. In experimental infection, the isolates produced only mild hepatitis in ICR mice treated with cortisone. By cross‐neutralization test, the nude isolate reacted closely with the virus from C3H mice but not with the virus from cancer cell line. The isolates from nude and C3H mice produced experimentally wasting disease with necrotic hepatitis in nude mice. These findings suggest that wasting disease in nude mice might be caused by low‐virulent mouse coronavirus shed in feces from C3H mice introduced before the outbreak of disease.
Iscom (Immunostimulating Complex) Vaccines Containing Mono‐ or Polyvalent Pili of Enterotoxigenic E. coli; Immune Response of Rabbit and Swine
Authors: Nagy Bèla , Höglund Stefan , Morein Bror 
Published: May 2010

SummaryIscom (immunostimulating complex) vaccines were prepared to contain K88ab, K88ac, K99 and 987P pili (fimbriae) of enterotoxogenic E. coli bacteria as monovalent or quadrivalent preparations.The iscoms injected into rabbits and into pigs elicited similar or higher immune response in both animal species than the oil adjuvanted vaccine containing about 5 times more of the same pilus protein. It is concluded that inclusion of pili into iscoms results in immunogenic preparations likely worth pursuing for vaccine production against enterotoxic colibacillosis of newborn pigs. The iscoms did not induce local reaction at the injection sites in contrast to the oil adjuvanted vaccines.
Fusobacterium Necrophorum Hemolysin in Bovine Hepatic Abscess
Authors: Kanoe Masamitsu 
Published: May 2010

SummaryAn immunofluorescence study was made on bovine hepatic abscess containing Fusobacterium necrophorum predominantly. The abscess section stained with anti F. necrophorum hemolysin serum demonstrated fluorescence which formed irregular and granular shapes. Actinomyces pyogenes isolates from the abscess were not stained with the serum. These findings suggest that the bacterial hemolysin contributes to the formation of the hepatic abscess during an infection with F. necrophorum.
Phallus‐Inflammation of Ganders: Clinical Observations and Comparative Bacteriological Examinations of Healthy and Altered Organs
Authors: Behr K.‐P. , Hinz K.‐H. , Rottmann Sabine 
Published: May 2010

SummaryThe examination of acute or chronically altered phallus‐tissues of ganders revealed microorganisms belonging to the genera Mycoplasma, Bacteroides, Clostridium, Streptococcus, Micrococcus, Staphylococcus, Lactobacillus, Corynebacterium, Escherichia, Campylobacter, Proteus, Pseudo‐monas and Candida and to the family Pasteurellaceae, isolated in different frequencies. Bacteriologic examinations of the phallus‐tissues and cloacal mucous membranes of healthy juvenile ganders showed microorganisms of the same genera or family, except Mycoplasma and Candida spp.Pathogenesis and possibilities of treating phallus‐inflammations in ganders are discussed.
A Preliminary Investigation on the Latency of the Goat Herpesvirus BHV‐6
Authors: Papanastasopoulou M. , Koptopoulos G. , Lekkas S. , Georgakopoulou E. , Papadopoulos O. 
Published: May 2010

SummaryAn experiment was designed for a preliminary investigation of the caprine herpesvirus BHV‐6 latency. The experiment was carried out in two steps. In the first, the virus inoculated six kids and the control, showed a mild infection and excreted the virus for 4—12 days. The second part started about one month after the cease of all symptoms and the virus isolations of the first part. No immunosuppression and therefore no virus reactivation could be achieved after eight daily administered injections of dexamethasone (0.15–0.50mg/kg BW), as it was judged by the hematological, virological, pathological and serological findings.
Characterization of a Murine IgG3 Monoclonal Antibody against Chlamydia‐Specific Lipopolysaccharide and its Purification by Affinity Chromatography on IgG Fc‐Receptors from Streptococcus Dysgalactiae
Authors: Thiele D. , Lämmler C. , Sting R. , Krauss H. 
Published: May 2010

SummaryPurification of a murine IgG3 monoclonal antibody (Mab 22) directed against an epitope of Chlamydia‐specific lipopolysaccharide by affinity chromatography on recently described solid phase IgG Fc‐receptors from Streptococcus dysgalactiae is reported. SDS‐PAGE studies revealed the purity of the eluted antibody. The purified Mab 22 was characterized by determination of class, subclass and light chain‐type, and by dot tests and immunoblot analysis.
Detection of Virus in Connection with “European Brown Hare Syndrome” in Hesse, F.R.G.
Authors: Biermann Ursula , Krauss H. 
Published: May 2010

SummaryMaterials from the liver of a wild‐living hare (Lepus europeus pallas) which had died from “European Brown Hare Syndrome” (EBHS) and of two hares kept in captivity which had been experimentally infected with the same material and died after two days with the classical signs of EBHS (Eskens and Volmer, 1989) were investigated for the presence of virus particles by electron microscopy using the negative contrast technique. Virus particles of 32 nm in diameter were found in all three samples investigated. The same particles were detected in the filtered inoculum used for experimental infection and in the supernatant of the first three passages of feline embryonic cell cultures. Haemagglutination or haemadsorption could not be achieved with the material investigated.
Gastrin and Pepsinogen Changes During anOstertagia ostertagiChallenge Infection in Calves
Authors: Hilderson H. , Dorny P. , Berghen P. , Vercruysse J. , Fransen J. , Braem L. 
Published: May 2010

SummaryDaily changes in serum gastrin and pepsinogen concentration have been studied during two types of infection withOstertagia ostertagiin calves. In a first experiment two calves were trickle infected (10 times 10,000 L3Ostertagia) and two animals received a single infection of 100,000 L3Ostertagia. Gastrin and pepsinogen changes are discussed in relation to adult wormburdens.The second experiment involved 8 calves and was designed to investigate pepsinogen and gastrin changes following a challenge infection in previously sensitized calves.The high dosed group was infected with 5,000 L3O. ostertagiduring 30 days, the low dosed group received 500 L3O. ostertagiand group 3 served as uninfected control. At day 41 post infection all animals were treated with oxfendazole and on day 61 challenged with 100,000 L3O. ostertagi. Only in the high dosed group a distinct pepsinogen and gastrin reaction was noticed. Both parameters dropped to almost preinfection levels after treatment. Two days post challenge a moderate rise (± 1,000 mU tyr) of the pepsinogen concentration was observed in the previously infected animals and gastrin showed a temporary slight increase in several animals 8 to 10 days post challenge. The effect of treatment and challenge infection is discussed in relation to gastrin and pepsinogen changes and immunity.
Comparative Studies of BHV‐1 Variants by In Vivo — In Vitro Tests
Authors: Bratanich A. C. , Sardi S. I. , Smitsaart E. N. , Schudel A. A. 
Published: May 2010

SummaryThe new encephalitogenic BHV‐1.3 and previously characterized BHV‐1 strains were studied with reference to their immunogenic and protective potency and their antigenic relationships using “in vitro” and “in vivo” tests.The “in vitro” results obtained by neutralization kinetics showed that the Los Angeles (LA) strain (BHV‐1.1) and a vaginal isolate L‐114 strain (BHV‐1.2) had antigenic similarities. Conversely, the behavior of the encephalitogenic strain A‐663 (BHV‐1.3), was significantly distinct.The “in vivo” protection test was carried out in calves using LA and A‐663 strains. Post‐vaccination antibodies and challenge with A‐663 strain showed that the immunogenic behavior and protective capacities of both strains were similar.Neutralization kinetics differences between BHV‐1.1 and BHV‐1.3 did not alter the “in vivo” protection against BHV‐1.3 challenge.
Detection of Bovine Rhinotracheitis Virus Antibody by Neutralizing Test and ELISA in Experimentally Infected Rabbits
Authors: Honda Eiichi , Taniguchi Takahide , Watanabe Makoto , Kumagai Tetsuo 
Published: May 2010

SummaryThe antibody response of rabbit to infectious bovine rhinotracheitis (IBR) virus was examined. Two rabbits were inoculated with IBR virus strain Los Angeles into the trachea and intravenously, and intravenously two times, respectively. The patterns of antibody titers in the rabbits measured by neutralization test and ELISA were similar to those in the case of bovine. The antibody was detected after the inoculation, and much more antibody was detected after the second inoculation. The fact suggests that rabbits are a very useful laboratory animal for IBR virus infection studies.
Treatment of Chronic Stomatitis of Cats by Local Paramunization with PIND‐ORF
Authors: Mayr Barbara , Deininger Susanne , Büttner M. 
Published: May 2010

Summary33 cats, suffering from chronic stomatitis, were treated with orally given paramunity inducer PIND‐ORF (local paramunization). As a control 39 cats in the same practice were treated with other conventional methods.The reconvalescence rate (healing without rezidives) of experimental animals was 42%. From control animals only 13% reached this status. Oral paramunization with PIND‐ORF is recommended as an alternative treatment for hitherto existing therapeutic measures against chronic stomatitis.
Effect of Insect Control on the Occurrence and Distribution of Streptothricosis Lesions on Cattle
Authors: Hadrill D.J. , Morrow A. N. , Heron I. D. , Robinson J. L. 
Published: May 2010

SummaryTrials were set up to ascertain whether the observed occurrence of streptothricosis lesions at dorsal sites on cattle in Antigua was related to the feeding activity of flies. Animals in two herds were treated with insecticide while a third herd served as an untreated control group. There was no significant difference in the occurrence or distribution of lesions on animals in all the three herds over a two month observation period except that a higher proportion of animals in one of the treated herds was affected at the end of the study.
Parasitologische, klinische, hämatologische und serologische Befunde bei Welpen nach galaktogener Infektion mit Ancylostoma caninum ERCOLANI 1859 (Ancylostomidae)
Authors: Gottstein Barbara , Ising Susanne , Stoye M. 
Published: May 2010

SummaryParasitological, clinical, hematological and serological findings in puppies after lactogenic infection with Ancylostoma caninum ERCOLANI 1859 (Ancylostomidae)The correlation between intensity of Ancylostoma caninum infections in bitches and the intensity of lactogenic infections and clinical signs of their puppies was investigated. On average, 825, 1,867 or 2,125 specimens were observed in litters of two bitches inoculated with 5,000, 10,000 or 20,000 third stage larvae (LIII) respectively, at the day of conception. Adverse effects of the infection on the growth and behaviour of the puppies were observed after onset of their second week of life: 11/ 27 puppies died during the fourth week. The body weight of puppies surviving 28 days was up to 750 g less than that of uninfected controls. Eosinophilia, erythroblastosis and microcytotic, hypochromic anemia developed in all puppies during their first four weeks. The IFA test (LIIIantigen) was positive for only 5/18 heavily infected puppies after uptake of colostrum.ZusammenfassungDie vorliegenden Untersuchungen erfolgten mit der Zielsetzung, zu prüfen, in welchem Maß der Umfang galaktogener Infektionen mit Ancylostoma caninum bei Welpen von der Infektionsstärke ihrer Muttertiere abhängig ist und welche Wirkung die Infektion bei den Jungtieren zeigt.Bei Welpen von Hündinnen, die am Tage der Konzeption perkutan mit 5000, 10 000 und 20 000 dritten Larven von Ancylostoma caninum infiziert worden waren, wurden im Mittel 824,5, 1866,5 und 2124,5 Exemplare von Ancylostoma caninum pro Wurf nachgewiesen.Einflüsse der Infektion auf die körperliche Entwicklung und das Verhalten der Welpen wurden von der 2. Lebenswoche an erkennbar. Ein Teil der massiv infizierten Jungtiere verendete während der 4. Lebenswoche. Welpen, die bis zum 28. Tag überlebt hatten, wogen zu dieser Zeit bis zu 750 g weniger als gleichaltrige nichtinfizierte Tiere.Bei allen Welpen kam es in den ersten Lebenswochen zu Eosinophilie, mikrozytärer, hypochromer Anämie und Erythroblastose. Antikörper gegen dritte Larven von Ancylostoma caninum waren mit der indirekten Fluoreszenzantikörpertechnik nur bei einigen der massiv infizierten Welpen nach Aufnahme von Kolostrum nachweisbar.
On the Pathogenesis of Anaemia and Leukopenia in Filarial (Litomosoides carinii) Infection of Mastomys natalensis
Authors: Ziegler Claudia , Käufer‐Weiss Ilse , Zahner H. 
Published: May 2010

SummaryMastomys natalensis infected with the filarial parasite Litomosoides carinii show anaemia and leukopenia. Alterations start with the onset of microfilaraemia. Anaemia is temporally macrocytic (up to 80 days after infection), subsequently normocytic and hypochromic, accompanied by reticulocytosis. Increased intravascular haemolysis (i) and functional disorders of the haemopoetic system (ii) are involved in the pathogenesis. i: Circulating erythrocytes showed increased osmofragility. Hypoglycaemia demonstrated in parasitaemic animals may be one reason. ii: Histological and electron microscopical investigations of the bone marrow revealed markedly enhanced haemopoiesis in infected animals. However, a high proportion of cells was found pathologically altered already beginning in the late prepatency and increasing in the further course of infection. Thus, dyshaemopoiesis may result in the production of morphologically and functionally aberrant cells which are rapidly eliminated by the MPS which is highly activated in L. carinii infected M. natalensis.
Carp Erythrodermatitis (CE) due to an Aeromonas hydrophila Infection
Authors: Sioutas S. , Hoffmann R. W. , Pfeil‐Putzien C. , Fischer‐Scherl Th. 
Published: May 2010

SummaryIn November 1987 high losses of carp (Cyprinus carpio) with the main symptom of skin ulcera were observed in a farm in northern Greece.Sixty‐six isolates of bacteria, characterized mainly as Aeromonas hydrophila or Pseudomonas spp. could be isolated from lesions of diseased fish. Transmission experiments with these isolates using mirror carp showed that Aeromonas hydrophila strains induced identical clinical and pathological pictures after intra‐ or subcutaneous injection. Extracts of these Aeromonas hydrophila isolates, as well as a supernatant of culture bouillon were toxic for carp and mice, indicating the presence of endo‐ and exotoxins. The results prove that carp erythrodermatitis (CE) may be caused by different bacteria, mainly including A. hydrophila.
A Field Study of Mycoplasma Bovis Infection in Cattle
Authors: Feenstra A. , Madsen E. Bisgaard , Friis N. F. , Meyling A. , Ahrens P. 
Published: May 2010

SummaryAfter an outbreak of mastitis in cattle caused byMycoplasma bovisa study was made in 5 herds with recent cases (principal herds) and in 4 control herds. In the principal herds,M. boviswas isolated from milk samples, nasal swabs, and from one vaginal swab.M. boviswas also isolated from nasal swabs of calves in 2 of the 4 control herds, whereas all milk samples and vaginal swabs from the control herds were negative.Evaluation of serum antibody titres toM. bovisamong non‐mastitic animals of 3 principal herds and 1 control herd showed no difference in distribution of the titre values, which generally were low. However, cows excretingM. bovisin the milk had high antibody titres. The way of introduction to the herds and the spread of the infection within the herds could not be established by the study, which was supplemented by a DNA restriction fragment analysis of a number ofM. bovisisolates.
Nachweis von Antikörpern gegen Larven von Anisakis simplex beim Seelachs Pollachius virens mittels ELISA
Authors: Priebe K. , Huber C. , Märtlbauer E. , Terplan G. 
Published: May 2010

SummaryDetection of antibodies against larvae of Anisakis simplex in saithe Pollachius virens by ELISAFrom the fact that older saithes (more than 5 years old) are showing significantly lower prevalence of attack by Anisakis larvae in lateral muscle than younger saithes (3–4 years old), the question arises if this phenomenon is based on a specific immune response. Therefore we have investigated serum samples from saithes of different ages by using an indirect ELISA to estimate the antibody‐titer against excretory‐secretory Anisakis antigen.Results showing a moderate correlation (r = 0.66) between the hight of titer and the age of underfeeding saithes (post spawning) and a close correlation (r = 0.93) of saithes in an optimal condition (pre spawning).It may be concluded that the migration‐distance and the lifetime of Anisakis larvae in lateralmuscle is influenced by a specific immune response which increases with the age of the saithes.ZusammenfassungDie Befallstärke des Seitenmuskels mit Anisakislarven ist bei älteren Seelachsen deutlich niedriger als bei 3–4 jährigen Seelachsen. Zur Klärung der Frage, ob dies auf einer Immunreaktion gegen die parenteral vorkommenden Nematodenlarven beruht, wurden Serumproben von Seelachsen verschiedenen Alters mittels indirekten ELISA auf das Vorhandensein von Antikörpern gegen exkretorischsekretorisches Anisakis‐Antigen untersucht. Es zeigte sich bei abgemagerten Färöer‐Seelachsen eine mäßige (r = 0,66) und bei gut genährten Hebriden‐Seelachsen eine enge (r = 0,93) Korrelation zwischen der Höhe der Serum‐Titer gegen Anisakis‐Antigen und dem Lebensalter der Fische. Diese Ergebnisse deuten darauf hin, daß der Seitenmuskel von mehr als 5 Jahre alten Seelachsen unter anderem deswegen nur selten mit Anisakislarven befallen ist, weil unter Beteiligung von Immunvorgängen mit steigendem Lebensalter die Migrationsdistanz und die Lebensdauer der Anisakislarven in der Muskulatur verkürzt werden.
Mycobacterial Antigen Detection by Immunohistochemistry in Goat Paratuberculosis
Authors: Navarro J. A. , Bernabé A. , Gómez M. A. , Sánchez J. , Gomez S. 
Published: May 2010

SummaryAn immunóhistochemical method (PAP) for a microscopic diagnosis of paratuberculosis in goats is described as an alternative method to the Ziehl‐Neelsen technique. Mycobacterial antigens are only found in enteric and mesenteric lymph node lesions. This method is the most sensible, particularly in cases in which there are no paratuberculosis lesions and the Ziehl‐Neelsen technique shows no acid‐fast bacilli (AFB).ZusammenfassungDetección de antigenos micobacterianos mediante inmunohistoquímica en paratuberculosis caprinaSe estudia la aplicación de una técnica inmunocitoquímica (PAP) con la utilización de un suero anti‐Mycobacterium paratuberculosiscomo método alternativo al Ziehl‐Neelsen en el diagnóstico microscópico de la paratuberculosis en cabras. Se detectaron antigenos micobacterianos sólo en lesiones entéricas y de ganglios mesentéricos, siendo este método particularmente eficaz en aquellos casos donde no existen lesiones tipicas de paratuberculosis y el método de Ziehl‐Neelsen puede no evidenciar bacilos ácido‐alcohol resistentes.Palabras clave: Paratuberculosis, Enfermedad de Johne, Inmunohistoquímica, Cabra.
Economic and Production Repercussions of Deworming by Pulse Release Device and Oral Suspension in Grazing Double‐Muscled Fattening Cattle
Authors: Genicot B. , Mouligneau F. , Lekeux P. 
Published: May 2010

SummaryIn this field trial, the repercussions of 2 administration forms of oxfendazole, namely a single administration of a front‐loaded device (group 1; n = 18) and a repeated administration of a 90.6 per cent oral suspension (group 2; n=18), were compared in first season‐grazing double‐muscled fattening bulls. The comparison was based on the production performances, the mean worm egg counts and the product‐ and labour‐costs.The daily body gains determined on the whole trial period, i.e. 1.312 ± 0.055 kg and 1.270 ± 0.056 kg for groups 1 and 2 respectively, and mean worm egg counts per gram (on days 0 and 130:200.0 ± 44.3 and 94.4 ± 27.4 for group 1; 366.7 ± 98.4 and 100.0 ± 33.6 for group 2) did not differ significantly between the two groups. However, the total costs were lower with a front‐loaded device (1,021 Belgian Francs) than with oral suspension (1,248 Belgian Francs per bull).
ELISA for Assessing Toxoplasma gondii Antibodies in Pigs
Authors: Bartoszcze M. , Krupa K. , Roszkowski J. 
Published: May 2010

SummaryThe ELISA technique was applied to assess Toxoplasma gondii antibodies in pigs. Among 925 swine examined 36.4 per cent of the animals were seropositive. Serum titres ranged from 100 to 3,200.
Immunohistochemical Detection of Chlamydiae in Formalin‐Fixed Tissue Sections: Comparison of a Monoclonal Antibody with Yolk Derived Antibodies (IgY)
Authors: Kunz Ursula Straumann , Pospischil A. , Paccaud M. F. 
Published: May 2010

SummaryImmunohistological detection of chlamydiae in formalin‐fixed and paraffin‐embedded sections of various organs from several species is described. In a retrospective study, two antisera, a commercially‐available monoclonal murine antibody (IgMur) and vitelline immunoglobulins (IgY), extracted from the egg yolk of immunized hens, were compared and tested for their applicability under routine condition. Both antisera were applied to tissues from which chlamydiae had been isolated or in which the presence of chlamydiae had been suspected in specially stained sections. Antigen labelling was optimal with the monoclonal antibody. Vitelline immunoglobulins produced some unspecific reactions, especially in lung tissue sections. Because of the antigenic relationship between the vitelline antibodies and tissues of birds, IgY are not suitable for the detection of psittacosis on avian substrates, when using an indirect immunological method. Staining in other tissues e. g. intestine or placenta was of equal quality as that attained with monoclonal antibodies. Depending on the advantages and disadvantages in every individual case, one of the two antibodies may be chosen for further studies. Vitelline antibodies should be preferred with respect to animal welfare.