bluuebunny/crossref_metadata_2025_split huggingface dataset:
How to manage an unresectable or recurrent sialoblastoma
Authors: Saribeyoglu Ebru Tugrul , Devecioglu Omer , Karakas Zeynep , Anak Sema , Unuvar Aysegul , Agaoglu Leyla , Celik Aladdin , Gulluoglu Mine , Bilgic Bilge 
Published: April 2010

AbstractOnly 2–5% of all salivary gland tumors occur in children. Sialoblastoma is an extremely rare salivary gland tumor diagnosed at birth or shortly thereafter with significant variability in histological range and clinical course, so that it may be difficult to predict the most appropriate therapy. In cases where surgical removal is not curative or technically feasible, chemotherapy may be attempted. We report herein a patient with progression of a huge partially resected sialoblastoma who was successfully treated with chemotherapy. Systemic chemotherapy with vincristine, actinomycin D, and cyclophosphamide (VAC) seems to be an effective adjuvant or neoadjuvant treatment option for unresectable or recurrent sialoblastoma. Pediatr Blood Cancer. 2010;55:374–376. © 2010 Wiley–Liss, Inc.
Potential response to curcumin in infantile hemangioendothelioma of the liver
Authors: Hassell Lewis A. , Roanh Le Dinh 
Published: April 2010

AbstractA case of life‐threatening infantile hemangioendothelioma of the liver in a 6‐month‐old infant treated successfully with the dietary supplement curcumin is reported, with 6‐year follow‐up. Implications for pathogenesis based on sites of action of curcumin are considered. Pediatr Blood Cancer. 2010;55:377–379. © 2010 Wiley–Liss, Inc.
Low PAPP‐A in the first trimester is associated with reduced fetal growth rate prior to gestational week 20
Authors: Salvig J. D. , Kirkegaard I. , Winding T. N. , Henriksen T. B. , Tørring N. , Uldbjerg N. 
Published: April 2010

AbstractObjectiveTo evaluate the association between maternal pregnancy‐associated plasma protein‐A (PAPP‐A) and fetal growth from the first to the second trimester.MethodsA prospective cohort study including 8347 pregnant women attending prenatal care at Aarhus University Hospital were conducted. PAPP‐A was measured during 8 to 14 gestational weeks. Fetal growth between the two scans in the first and second trimesters was estimated by (GA20− GA12)/Dayscalendar, where GA12 reflects gestational age in days calculated from crown‐rump length at a 12‐week scan, GA20 reflects gestational age in days calculated from biparietal diameter at a 20‐week scan, and Dayscalendar reflects the number of calendar days between the two scans.ResultsFetal growth rate from the first to the second trimester was correlated with PAPP‐A, with a regression coefficient of 0.009 (95% CI, 0.007–0.012, P < 0.001). PAPP‐A below 0.30 MoM was associated with a fetal growth rate below the tenth centile, with an adjusted OR of 2.05 (95% CI, 1.24–3.38).ConclusionLow levels of PAPP‐A are associated not only with low birth weight at term but also with slower fetal growth prior to 20 weeks of gestation. Copyright © 2010 John Wiley & Sons, Ltd.
Prevention of severe thalassemia in northeast Thailand: 16 years of experience at a single university center
Authors: Yamsri Supawadee , Sanchaisuriya Kanokwan , Fucharoen Goonnapa , Sae‐ung Nattaya , Ratanasiri Thawalwong , Fucharoen Supan 
Published: April 2010

AbstractObjectiveTo demonstrate the performance of thalassemia prevention in northeast Thailand during 1993–2008.MethodsRetrospective data from 1422 at‐risk couples who attended from January 1993 to December 2008 were studied. All couples were suspected at‐risk couples based on initial screening using standard protocols. Three thalassemia carrier types including α0‐thalassemia, β‐thalassemia and hemoglobin E were identified using standard methods. Data on prenatal diagnosis were collected.ResultsOf the 1422 positive‐screened couples, 1254 (88.2%) were diagnosed as true‐positive couples. After DNA analysis, 968 of 1254 (77.2%) resulted at risk for three types of severe conditions being hemoglobin E–β‐thalassemia disease (640/968, 66.1%), homozygous α0‐thalassemia (304/968, 31.4%) and homozygous β‐thalassemia (11/968, 1.1%). The remaining 1.3% of the couples were at risk for more than one disease. After genetic counseling, prenatal diagnosis was performed on 756 couples (78.1%). The proportions of affected fetuses, thalassemia carriers and unaffected fetuses were 26.9, 50.0 and 23.0%, respectively.ConclusionImplementation of a prevention and control program accompanying with a referral system for prenatal diagnosis is technically feasible in northeast Thailand and a large number of severe thalassemia diseases have been prevented during the past 16 years of operation. Copyright © 2010 John Wiley & Sons, Ltd.
Offspring birth weight in second‐generation ‘small for gestational age’ infants
Authors: Farina Antonio , Dini Benedetta , Mattioli Mara , Rosa Stefania , Rizzo Nicola 
Published: April 2010

AbstractObjectiveTo quantify the intergenerational effects on intrauterine growth in pregnant women who were themselves small for gestational age (SGA) at birth.MethodsA retrospective case control was carried out on infants born in the period 1 January 2009 to 31 March 2009 at the Sant'Orsola Hospital, Bologna. Out of 958 infants, 707 met the criteria for inclusion in the study.ResultsAfter calculation of the percentiles stratified for gestational age at delivery, 68 cases were classified as SGA. Logistic regression analysis was used as the multivariable tool for calculating the bearing of each covariable on the outcome (SGA), including smoking and the presence of an SGA in previous pregnancies. A total of 28.6% of the infants of SGA women were SGA compared with 8.6% of the children of non‐SGA women (p‐value < 0.01). The multivariable odds ratio was 2.61 (95% CI = 1.16–5.86) for women who were SGA to have SGA children. The extra risk of being SGA in the actual pregnancy when the mother was SGA was estimated at about 7%.ConclusionReduced intrauterine growth of the mother is a risk factor for reduced intrauterine growth of her children. Copyright © 2010 John Wiley & Sons, Ltd.
Comparative proteomic analysis of rat aorta in a subtotal nephrectomy model
Authors: Lin Yao‐Ping , Hsu Meng ‐ Erh , Chiou Yi‐Ying , Hsu Hung‐Yi , Tsai Hiao‐Chien , Peng Yu‐Ju , Lu Chi‐Yu , Pan Chien‐Yuan , Yu Wen‐Chung , Chen Chen‐Huan , Chi Chin‐Wen , Lin Chao‐Hsiung 
Published: April 2010

AbstractAlthough accelerated atherosclerosis and arteriosclerosis are the main causes of cardiovascular morbidity and mortality in chronic kidney disease (CKD) patients, the molecular pathogenesis remains largely obscure. Our study of the aortic function in a typical CKD model of subtotal nephrectomy (SNX) rats demonstrated phenotypes that resemble CKD patients with aortic stiffness. The 2‐DE analysis of rat aortas followed by MS identified 29 up‐regulated and 53 down‐regulated proteins in SNX rats. Further Western blot and immunohistochemistry analyses validated the decreased HSP27 and increased milk fat globule epidermal growth factor‐8 (MFG‐E8) in SNX rats. Functional classification of differential protein profiles using KOGnitor revealed that the two major categories involved in aortic stiffness are posttranslational modification, protein turnover, chaperones (23%) and cytoskeleton (21%). Ingenuity Pathway Analysis highlighted cellular assembly and organization, and cardiovascular system development and function as the two most relevant pathways. Among the identified proteins, the clinical significance of the secreted protein MFG‐E8 was confirmed in 50 CKD patients, showing that increased serum MFG‐E8 level is positively related to aortic stiffness and renal function impairment. Drug interventions with an inhibitor of the angiotensin converting enzyme, enalapril, in SNX rats improved aortic stiffness and decreased MFG‐E8 depositions. Together, our studies provide a repertoire of potential biomarkers related to the aortic stiffness in CKD.
Quantitative analysis of the secretome of TGF‐β signaling‐deficient mammary fibroblasts
Authors: Xu Baogang J. , Yan Wenwei , Jovanovic Bojana , An Angel Q. , Cheng Nikki , Aakre Mary E. , Yi Yajun , Eng Jimmy , Link Andrew J. , Moses Harold L. 
Published: April 2010

AbstractTransforming growth factor β (TGF‐β) is a master regulator of autocrine and paracrine signaling pathways between a tumor and its microenvironment. Decreased expression of TGF‐β type II receptor (TβRII) in stromal cells is associated with increased tumor metastasis and shorter patient survival. In this study, SILAC quantitative proteomics was used to identify differentially externalized proteins in the conditioned media from the mammary fibroblasts with or without intact TβRII. Over 1000 proteins were identified and their relative differential levels were quantified. Immunoassays were used to further validate identification and quantification of the proteomic results. Differential expression was detected for various extracellular proteins, including proteases and their inhibitors, growth factors, cytokines, and extracellular matrix proteins. CXCL10, a cytokine found to be up‐regulated in the TβRII knockout mammary fibroblasts, is shown to directly stimulate breast tumor cell proliferation and migration. Overall, this study revealed hundreds of specific extracellular protein changes modulated by deletion of TβRII in mammary fibroblasts, which may play important roles in the tumor microenvironment. These results warrant further investigation into the effects of inhibiting the TGF‐β signaling pathway in fibroblasts because systemic inhibition of TGF‐β signaling pathways is being considered as a potential cancer therapy.
Thioredoxin targets in Arabidopsis roots
Authors: Marchand Christophe H. , Vanacker Hélène , Collin Valérie , Issakidis‐Bourguet Emmanuelle , Maréchal Pierre Le , Decottignies Paulette 
Published: April 2010

AbstractThe importance of redox‐regulation in Arabidopsis thaliana roots has been investigated through the identification of the proteins interacting with thioredoxin (TRX), an ubiquitous thiol‐disulfide reductase. We have applied a proteomic approach based on affinity chromatography on a monocysteinic mutant of plastidial y‐type TRX used as a bait to trap putative partners in a crude extract of root proteins. Seventy‐two proteins have been identified, functioning mainly in metabolism, detoxification and response to stress, protein processing and signal transduction. This study allowed us to isolate 24 putative new targets and to propose the mevalonic acid‐dependent biosynthesis of isoprenoids as a new redox‐mediated process. The redox‐regulation of phenylpropanoid biosynthesis is also suggested, three enzymes of this pathway being retained on the column. We also provided experimental evidence that phenylammonia‐lyase was enzymatically more active when reduced by TRXy in root crude extract. Among the high number of partners involved in defense against stress we isolated from the column, we focused on plastidial monodehydroascorbate reductase and showed that its activity was dramatically increased in vitro in the presence of DTT‐reduced TRXy1 in root crude extracts. Our data strongly suggest that TRXy1 could be the physiological regulator of monodehydroascorbate reductase in root plastids.
Understanding the cancer pain experience in American Indians of the Northern Plains
Authors: Haozous Emily A. , Knobf M. Tish , Brant Jeannine M. 
Published: April 2010

AbstractObjective: The purpose of this study was to explore the experience of cancer pain in American Indians (AI) from the Northern Plains region of the United States.Design: This was a qualitative descriptive study.Setting: Southeastern Montana.Participants: The sample consisted of 10 participants from Northern Plains AI tribes/nations, aged 31–75 years, with both solid and hematologic malignancies.Main outcome measures: Data were produced through semi‐structured interviews and through the use of the Brief Pain Inventory‐Short Form.Results: Participants reported mild‐to‐moderate pain on a daily basis, and pain negatively interfered with their daily activities and enjoyment of life. The average pain score at the time of interview was 4.2 out of 10. Participants described both somatic and neuropathic pain. Participants reported psychosocial distress through feelings of guilt, depression, and isolation related to their cancer, and they relied strongly on prayer and traditional medicine in coping with pain. Dominant themes were ‘Isolating Within’ and ‘Making Connections’.Conclusions: This study suggests that there is very little difference between AI and other cultural groups regarding cancer pain in the description of pain, patterns, and types of pain management. However, this study identified notable differences in the high degree of isolation reported by AI, their desire to endure pain with stoicism, their intense desire to protect their privacy, and their distrust of outsiders. Findings from this study will inform future research on pain and pain interventions in AI patients with cancer. Copyright © 2010 John Wiley & Sons, Ltd.
Cancer patients' trust in their physician—a review
Authors: Hillen Marij A. , de Haes Hanneke C. J. M. , Smets Ellen M. A. 
Published: April 2010

AbstractObjective: Patient's trust in their physician is crucial for desirable treatment outcomes such as satisfaction and adherence. In oncology, trust is possibly even more essential, due to the life‐threatening nature of cancer. A review was undertaken of the current knowledge of the conceptualization, assessment, correlates, and consequences of cancer patients' trust in their physician.Methods: The empirical literature published in peer‐reviewed journals between October 1988 and October 2008 was searched, employing all combinations and variations of the following keywords: trust, physician–patient relations, and cancer.Results: The search identified 45 relevant papers, only 11 of which drew attention to the conceptualization of trust, and 5 of which focused on trust as the primary subject of interest. Trust in physicians was strong overall. Patients' trust appeared to be enhanced by the physician's perceived technical competence, honesty, and patient‐centred behaviour. A trusting relationship between patient and physician resulted in facilitated communication and medical decision making, a decrease of patient fear, and better treatment adherence.Conclusions: A lack of focus on trust and the conceptualization thereof, strong methodological variations between studies and a possible publication bias lead us to conclude that cancer patients' trust in their physician deserves more systematic, theoretically based, research attention. Consequently, studies are needed aimed at gaining a thorough understanding of the nature and impact of cancer patients' trust in their physician, and how the interaction between physician and patient may contribute to such trust. Copyright © 2010 John Wiley & Sons, Ltd.
Purification of Escherichia coli RNA polymerase using a self‐cleaving elastin‐like polypeptide tag
Authors: Fong Baley A. , Gillies Alison R. , Ghazi Iraj , LeRoy Gary , Lee Kathleen C. , Westblade Lars F. , Wood David W. 
Published: April 2010

AbstractA self‐cleaving elastin‐like polypeptide (ELP) tag was used to purify the multisubunit Escherichia coli RNA polymerase (RNAP) via a simple, nonchromatographic method. To accomplish this, the RNAP α subunit was tagged with a self‐cleaving ELP‐intein tag and coexpressed with the β, β′, and ω subunits. The assembled RNAP was purified with its associated subunits, and was active and acquired at reasonable yield and purity. To remove residual polynucleotides bound to the purified RNAP, two polymer precipitation methods were investigated: polyethyleneimine (PEI) and polyethylene (PEG) precipitation. The PEG procedure was shown to enhance purity and was compatible with downstream ELP‐intein purification. Thus, this simple ELP‐based method should be applicable for the nonchromatographic purification of other recombinant, in vivo‐assembled multisubunit complexes in a single step. Further, the simplicity and low cost of this method will likely facilitate scale up for large‐scale production of additional multimeric protein targets. Finally, this technique may have utility in isolating protein interaction partners that associate with a given target.
Radiative recombination dynamics of CdSe/Zn(S,Se)/MgS quantum dots up to room temperature
Authors: Zaitsev S. V. , Arians R. , Kümmell T. , Bacher G. , Gust A. , Kruse C. , Hommel D. 
Published: April 2010

AbstractThe recombination dynamics and its temperature dependence are studied in detail in epitaxially grown CdSe/Zn(S,Se) quantum dots (QDs) with additional wide‐band MgS barriers. Such design allows to preserve a very high quantum yield and to track the QD recombination dynamics up to room temperature. At low temperatures, a fast initial decay ∼0.6 ns is observed which is followed by a long decay with a time constant ∼25 ns. The fast initial decay disappears with increasing temperature and above 100 K only a single‐exponential decay is observed with a decay time τ of 1.2–1.5 ns, which is weakly dependent on temperature up to 300 K. A two‐level model including bright and dark exciton states and temperature dependent spin‐flip between them describes well the experimental findings. According to the model, the long decay tail results from a thermally activated population of the bright exciton state from the energetically lowest dark state.
Fluorine‐doped ZnSe for applications in quantum information processing
Authors: Ladd T. D. , Sanaka K. , Yamamoto Y. , Pawlis A. , Lischka K. 
Published: April 2010

AbstractDevices based on group II–VI semiconductors, and in particular ZnSe, show several advantages for single‐photon sources in comparison to group III–V and group IV devices. Additionally, the spin system of the neutral 19F donor has strong potential as a quantum memory.Here, we summarize these advantages and some initial demonstrations of the promise of 19F:ZnSe for quantum information, including the observation of the interference of photons from independent single 19F:ZnSe emitters and the fabrication of 19F‐doped microdisk cavities showing low‐threshold lasing.
Robotic single‐incision transabdominal and transvaginal surgery: initial experience with intersecting robotic arms
Authors: Hagen Monika E. , Wagner Oliver J. , Inan Ihsan , Morel Philippe , Fasel Jean , Jacobsen Garth , Spivack Adam , Thompson Kari , Wong Brian , Fischer Lauren , Talamini Mark , Horgan Santiago 
Published: April 2010

AbstractBackgroundSingle‐incision laparoscopic and natural orifice translumenal endoscopic surgery (NOTES) are technically challenging methods. Robotics might have the potential to overcome such hurdles with computer technology.MethodsThe da Vinci® Standard and S System (Intuitive, Sunnyvale, USA) were used in human cadavers and pigs to perform single‐incision transabdominal and transvaginal surgery. Robotic arms were crossed and control‐switched to achieve intuitive control.ResultsIt was possible to perform robotic single‐incision laparoscopy in the typical, intuitive fashion. Transvaginal set‐up, including docking of the system and introduction of instruments into the abdominal cavity, was possible but no useful manipulation could be performed.ConclusionsWhile robotic NOTES with the da Vinci surgical system was not successful, robotic single‐incision surgery is feasible using the above set‐up. This new approach seems to offer the advantages of single‐incision surgery while maintaining the intuitive control of robotic surgery. Clinical application appears justified. Copyright © 2010 John Wiley & Sons, Ltd.
Robust control for uncertain nonlinear systems with state‐dependent control direction
Authors: Yang Zaiyue , Yam S. C. P. , Li L. K. , Wang Yiwen 
Published: April 2010

AbstractThe control direction is the multiplier of the control term. In this study, the control direction is considered as an unknown function of state and allowed to cross zero and change its sign smoothly. Based on the analysis of system dynamics at the points where the control direction is zero, a robust controller is proposed by integrating with a bounding function and a Nussbaum‐type gain. Under the proposed controller, the system approaches zero if zero is accessible, or to the accessible point closest to zero if zero is not accessible by any control. The control performance is illustrated by simulated examples. Copyright © 2010 John Wiley & Sons, Ltd.
Application of atomic force microscopy in bacterial research
Authors: Dorobantu Loredana S. , Gray Murray R. 
Published: April 2010

AbstractThe atomic force microscope (AFM) has evolved from an imaging device into a multifunctional and powerful toolkit for probing the nanostructures and surface components on the exterior of bacterial cells. Currently, the area of application spans a broad range of interesting fields from materials sciences, in which AFM has been used to deposit patterns of thiol‐functionalized molecules onto gold substrates, to biological sciences, in which AFM has been employed to study the undesirable bacterial adhesion to implants and catheters or the essential bacterial adhesion to contaminated soil or aquifers. The unique attribute of AFM is the ability to image bacterial surface features, to measure interaction forces of functionalized probes with these features, and to manipulate these features, for example, by measuring elongation forces under physiological conditions and at high lateral resolution (<1 Å). The first imaging studies showed the morphology of various biomolecules followed by rapid progress in visualizing whole bacterial cells. The AFM technique gradually developed into a lab‐on‐a‐tip allowing more quantitative analysis of bacterial samples in aqueous liquids and non‐contact modes. Recently, force spectroscopy modes, such as chemical force microscopy, single‐cell force spectroscopy, and single‐molecule force spectroscopy, have been used to map the spatial arrangement of chemical groups and electrical charges on bacterial surfaces, to measure cell–cell interactions, and to stretch biomolecules. In this review, we present the fascinating options offered by the rapid advances in AFM with emphasizes on bacterial research and provide a background for the exciting research articles to follow. SCANNING 32: 74–96, 2010. © 2010 Wiley Periodicals, Inc.
An assessment of gum‐based environmental enrichment for captive gummivorous primates
Authors: Huber Hillary F. , Lewis Kerrie P. 
Published: April 2010

AbstractIn the wild, many primates consume gums exuded from trees, and many species are gum specialists. In spite of this, few data exist concerning gum feeding in captivity. Using a web‐based survey of 46 zoos in 12 countries, we evaluated the extent to which zoos feed gum to primates. We found that although callitrichids and galagos receive gum‐based enrichment, cercopithecines generally do not. Environmental enrichment is important for stimulating naturalistic behavior to promote the psychological wellbeing of animals. Thus, gum‐based enrichment is important for captive gummivores. Our study highlights the need to improve environmental enrichment for captive gummivores, in particular that of cercopithecines. This is most striking for the patas monkey (Erythrocebus patas), an obligate gummivore. The exchange of ecological data between field research and captive settings is crucial, and is just one way primate caretakers can contribute to the conservation and welfare of some of our closest living relatives. Zoo Biol 30:71–78, 2011. © 2010 Wiley‐Liss, Inc.
Radiographic diagnosis of metabolic bone disease in captive bred mountain chicken frogs (Leptodactylus fallax)
Authors: King Jay D. , Muhlbauer Michael C. , James Arlington 
Published: April 2010

AbstractAsymptomatic captive bred and wild‐caught mountain chicken frogs (Leptodactylus fallax) were radiographed for evidence of metabolic bone disease (MBD). All 22 captive bred frogs had multiple folding fractures of long bones, decreased bone density, and cortical thinning, whereas none of the 11 wild‐caught frogs had any radiographic evidence of MBD. These findings suggest that the nutritional requirements of L. fallax need to be examined for captive management purposes. Zoo Biol 30:254–259, 2011. © 2010 Wiley‐Liss, Inc.