bluuebunny/crossref_metadata_2025_split huggingface dataset:
A bacterial artificial chromosome mouse model of amyotrophic lateral sclerosis manifests ‘space cadet syndrome’ on two FVB backgrounds
Authors: Badger Sophie E. , Coldicott Ian , Kyrgiou-Balli Ergita , Higginbottom Adrian , Moutin Chloé , Mohd Imran Kamallia , Day John C. , Cooper-Knock Johnathan , Mead Richard J. , Alix James J. P. 
Published: February 2025

ABSTRACT
C9orf72-related amyotrophic lateral sclerosis (ALS)/frontotemporal dementia (FTD) has proven difficult to model in mice. Liu et al. (2016) reported a bacterial artificial chromosome (BAC) transgenic mouse displaying behavioural, motor and pathological abnormalities. This was followed by multiple laboratories independently refuting and confirming phenotypes. A proposed explanation centred on the use of different FVB background lines (from The Jackson Laboratory and Janvier Labs). We studied C9orf72 BAC mice on both backgrounds and found significantly elevated levels of dipeptide repeat proteins, but no evidence of a transgene-associated phenotype. We observed seizures and a gradual decline in functional performance in transgenic and non-transgenic mice, irrespective of genetic background. The phenotype was in keeping with the so-called ‘space cadet syndrome’. Our findings indicate that the differences previously reported are not due to C9orf72 status and highlight the importance of using genetic backgrounds that do not confound interpretation of neurodegenerative phenotypes.
Dachsous is a key player in epithelial wound closure by modulating cell shape changes and tissue mechanics
Authors: Porfírio-Rodrigues Patrícia , Pereira Telmo , Jacinto Antonio , Carvalho Lara 
Published: February 2025

Epithelia are vital tissues in multicellular organisms, acting as barriers between external and internal environments. Simple epithelia, such as pg those in embryos and the adult gut, have the remarkable ability to repair wounds efficiently, making them ideal for studying epithelial repair mechanisms. In these tissues, wound closure involves the coordinated action of a contractile actomyosin cable at the wound edge and collective cell movements around the wound. However, the dynamics of cell-cell interactions during this process remain poorly understood.
Here, we demonstrate that Dachsous (Ds), an atypical cadherin associated with Planar Cell Polarity, is crucial for efficient epithelial repair in the Drosophila embryo. We show that the absence of Ds alters tissue mechanics and cell shape changes and rearrangements, leading to slower wound closure. Additionally, we reveal that Occluding Junctions are necessary for the proper apical localization of Ds, uncovering an unanticipated interaction between these two molecular complexes. This study identifies Ds as a novel key player in epithelial repair and highlights the need for further investigating the molecular mechanisms by which Ds modulates cell shape and tissue morphogenesis.
New findings of neogene tortoises Titanochelon kayadibiensis sp. nov. and Protestudo bessarabica (Riabinin, 1918) (Testudinidae) from the Miocene of western Turkey, with a review of fossil turtles of Turkey
Authors: Volker Karl Hans , Staesche Ulrich , Safi Amtyaz 
Published: February 2025

Fossil remains of turtles were discovered in various localities of Lower to Upper Miocene within the western part of Turkey. We revised the taxonomic position and describe the systematic paleontology, distribution, phylogeny and palaeo-environments of two Neogene taxa Titanochelon kayadibiensis sp. nov. and Protestudo bessarabica (Testudinidae) of Western Turkey and give an updated list of fossil turtles of Turkey which include Trionyx triunguis (ForskÃ¥l, 1775), Mauremys aristotelica Vlachos et al, 2019, Testudo marmorum Gaudry, 1862, Protestudo bessarabica Riabinin, 1918 and Titanochelon kayadibiensis sp.nov.Â Â
Healing Mind: Mental health, Indigenous healers and Traditional Medicine
Authors: Singh Ravinder , Kumar Upmesh 
Published: February 2025

Mental illnesses and other psychosocial disabilities are main source of burden for the family. About 13.7 per cent of general population of India has issues of mental illness or disorders and 10.6 per cent of these people need immediate intervention for the treatment followed by 10 per cent of the population has common mental disorders with 1.9 per cent people suffers with severe mental disorders. The mental morbidity is very high in urban areas. There is a higher prevalence of neurotic or stress-related disorders, schizophrenia and mood disorders. This situation may be due to fast-life styles, experiencing stress, complexities of living, a breakdown of support systems and challenges of economic instability. There are few population-level insights into the use of traditional healers and other forms of alternative care for the treatment of common mental disorders in our country as well as in South Asian and sub-Saharan Africa countries. Paper examines the extent to which alternative practitioners are consulted, and predictors of traditional healer visits for various neuropsychiatric diseases. Further it illuminates studies and national survey conducted on mental health on tribal communities and the role of traditional healers in the country. Paper raised the issue of mental illnesses as prevalent in the country and questions that do indigenous healing matters in healing the mind for better mental health in our country in first part of the paper besides analyzing recent survey, NMHS-2014. Then illuminates various mind healing ways and put it in question whether we can protect these healers and their medicines in existing IPR in part-II followed by traditional knowledge, medicines, National policy and cases of Indian patents and Intellectual Property Rights (IPR) in the last part where we conclude countries like Canada, South Africa or New Zeeland have supported for integration of traditional healers with main stream health care system. There are fears also generated for example in Canada that increasing acceptance of traditional healing may result into sea change. The lessons of these countries may be useful for our planning for integration of healers and their medicine in our country for effective resources utilization of the traditional healers and their medicines.Â Â Â
Advances in repetitive transcranial magnetic stimulation in the treatment of treatment-resistant depression: a comprehensive review
Authors: Xing Haoran , Liu Xiaolei , Zhang Yingying , Wu Jie , Li Mier , Ye Hong , Ding Yu , Wang Weiwei , Zheng Zhong , Bao Tianhao 
Published: February 2025

Abstract
Major depressive disorder is a common mental disorder, and a significant number of patients exhibit poor response to conventional antidepressant treatments, known as treatment-resistant depression (TRD). However, the definition of TRD globally remains unclear, impeding clinical research, treatment development, outcome implementation, and policy-making. A wealth of research confirms that rTMS demonstrates promising efficacy in TRD. This paper elucidates the definition of TRD, summarizes potential targets of rTMS for treating TRD, comprehensively elaborates on the unique mechanisms, efficacy, and side effects of rTMS in treating TRD, and outlines considerations for special populations receiving rTMS treatment for TRD as well as other treatment modalities for TRD. Through these studies, we aim to provide more scientifically grounded recommendations for patients undergoing rTMS treatment for TRD.
Motives for pornography use and women's sexual wellbeing: Insights from a 42-country study
Authors: Gewirtz-Meydan Ateret , Feder Hagit , Nagy Léna , Koós Mónika , Kraus Shane W. , Demetrovics Zsolt , Potenza Marc N. , Ballester-Arnal Rafael , Batthyány Dominik , Bergeron Sophie , Billieux Joël , Briken Peer , Burkauskas Julius , Cárdenas-López Georgina , Carvalho Joana , Castro-Calvo Jesús , Chen Lijun , Ciocca Giacomo , Corazza Ornella , Csako Rita I. , Fernandez David P. , Fernandez Elaine F. , Fujiwara Hironobu , Fuss Johannes , Gabrhelík Roman , Gjoneska Biljana , Gola Mateusz , Grubbs Joshua B. , Hashim Hashim T. , Islam Md. Saiful , Ismail Mustafa , Jiménez-Martínez Martha C. , Jurin Tanja , Kalina Ondrej , Klein Verena , Költő András , Lee Chih-Ting , Lee Sang-Kyu , Lewczuk Karol , Lin Chung-Ying , Lochner Christine , López-Alvarado Silvia , Lukavská Kateřina , Mayta-Tristán Percy , Miller Dan J. , Orosová Oľga , Orosz Gábor , _ _ , Ponce Fernando P. , Quintana Gonzalo R. , Quintero Garzola Gabriel C. , Ramos-Diaz Jano , Rigaud Kévin , Rousseau Ann , De Tubino Scanavino Marco , Schulmeyer Marion K. , Sharan Pratap , Shibata Mami , Shoib Sheikh , Sigre-Leirós Vera , Sniewski Luke , Spasovski Ognen , Steibliene Vesta , Stein Dan J. , Strizek Julian , Štulhofer Aleksandar , Ünsal Berk C. , Vaillancourt-Morel Marie-Pier , Van Hout Marie Claire , Bőthe Beáta 
Published: February 2025

AbstractBackground and aimsDespite a growing body of research on pornography use among women, there is a lack of understanding of the problematic versus non-problematic nature. The current study aimed to investigate the relationship between women's motivations for pornography use and sexual wellbeing using a cross-sectional, self-report survey design among participants from 42 countries.MethodsThe total sample included 82,243 participants, of whom 46,874 (57.0%) identified as women and were analyzed. The participants' age averaged at M = 29.67 years, with a standard deviation of SD = 10.11. Participants were asked to complete a questionnaire assessing their motivations for pornography use, as well as measures of sexual functioning, sexual desire, and sexual satisfaction.ResultsStudy results suggest that across cultures, women's motivations for pornography use are associated with their sexual wellbeing. Specifically, when women reported using pornography for their own pleasure or sexual curiosity, it was associated with fewer sexual functioning problems and higher sexual desire. Conversely, when women reported using pornography due to a lack of sexual satisfaction in their relationships, it was associated with more sexual functioning problems.Discussion and conclusionsThese findings highlight the need to consider the multifaceted nature of pornography use among women, including the usage motives, to fully understand associations with sexual wellbeing. Additionally, the study emphasizes the importance of conducting further research utilizing longitudinal designs, to establish the directionality between pornography use motivations and sexual wellbeing among women.
Development of the Conceptual Apparatus of Regional Ecosystems of Scientific Creativity: Challenge for Library Professionals
Authors: Sokolov S. V. 
Published: February 2025

The purpose of the article is to work out approaches to the creation of the conceptual apparatus for determining the conditions and directions of the development of ecosystems of scientific creativity in the conditions of progress of collaboration between scientific, library, educational and publishing sectors of the economy. The article reveals the methodology of the terminological approach, shows the importance of its combination with scientometric analysis. It presents the works of the Research Department of Library Science of the Institute of Scientific Information on Social Sciences of the Russian Academy of Sciences (INION RAN) to the creation of the conceptual apparatus of regional ecosystems of scientific creativity in such areas as the development of state standards and publication of abstract, information and analytical materials. The significance of the terminological standard "Bibliometric indicators. Terms and definitions, measurement structure", the first edition of which was prepared in accordance with the national standardization plan for 2024. The grounds for the creation of the standard, the main goals and objectives of its development are presented, the characteristics of the object of standardization are given, the scope of the standard and its technical and economic efficiency are shown. The terms related to the bibliometric assessment of young researchers’ activities, including foreign ones and the creation of the system of reliable information are noted. The importance of the INION RAN collection "Actual problems of library and information sciences" (2024) in the study of the modern foreign terminological system of scientific, library and publishing activities is shown. Attention is drawn to the presence of the subject index to the collection of 249 lexical units based on the analysis of 285 works on library and information sciences by American, German, English and Chinese researchers. The potential of the Youth Scientific Laboratory of the Institute of Scientific and Technical Information of the Donetsk People's Republic for elaborating the concept of forming regional ecosystems of scientific creativity is marked. Along with the study of the conceptual apparatus of the ecosystem of scientific creativity based on the terminological approach, the program for further monitoring and analysis of ecosystems of scientific creativity, as well as the list of terms, are presented.
Two-thirds of agricultural carbon and biodiversity loss occurs on one-third of the agricultural area
Authors: Liu Baoxiao , Behrens Paul , Sun Zhongxiao , Scherer Laura 
Published: February 2025

Abstract

Carbon storage and biodiversity loss are both heavily impacted by the food system. Here, we map and trace carbon and biodiversity loss in global agriculture along international supply chains at a high spatial and product resolution. While the northern hemisphere in general sees greater carbon loss and the southern higher biodiversity loss, the areas of highest carbon and biodiversity loss are often co-located, comprising a third of the total area and almost two-thirds of both total losses. Brazil is the largest net exporter for both losses, and China the largest net importer. Food consumption drives 83% of losses, of which animal-sourced foods drive 59% of carbon and 71% of biodiversity loss. Bovine meat and milk alone comprise 29% carbon and 41% biodiversity losses. The overlap between regions and products of high loss strengthen calls for joint efforts in carbon and biodiversity policy that involve both supply and demand interventions.
7-Day Risk Prediction for Major Adverse Cardiac Events in Acute Coronary Syndrome Patients
Authors: Moeswir Dede , Nasution Sally A , Gliselda Vika K , Alwi Idrus 
Published: February 2025

Abstract

Background: Major Adverse Cardiac Events (MACE) increase illness and death rate among patients with acute coronary syndrome (ACS). Prediction scores have been utilized as prognostic to predict MACE. This study aims to develop a simple risk score that is easy to calculate and applicable for identifying ACS patients at risk for MACE.
Methods: A retrospective cohort study involving 1,002 ACS patients in an intensive coronary care unit from January 1st, 2021, until July 31st, 2024. Sex, age, family history, diabetes, hemoglobin, leukocyte, creatinine, uric acid, cardiac enzyme, systolic blood pressure, heart rate, cardiac arrest, ST segment deviation, and Killip class were assessed as risk factors for MACE.
Results: MACE was found in 112 (9.21%) of ACS patients. Predictors such as female, leukocyte, creatinine, uric acid, cardiac enzyme, systolic blood pressure, heart rate, cardiac arrest, and Killip class in multivariate logistic regression analysis were associated with MACE with (RR 95% CI) 2.66 (1.35-5.25), 2.06 (1.02-4.16), 2.84 (1.43-5.66), 3.79 (1.90-7.54), 3.26 (1.51-7.05), 3.48 (1.57-7.70), 2.46 (1.20-5.01), 42.04 (18.90-93.51), and 6.31 (3.19-12.50), respectively. The best predictive accuracy was obtained by an area under the curve of 0.95, 95% CI, 0.93-0.97.
Conclusions: In ACS patients, the probability of MACE was found to be 3.6% for those with scores 0-6 and 83.5% for those with scores greater than 6, based on the following predictor factors: female (score 1), leukocytosis (score 1), elevated creatinine (score 1), hyperuricemia (score 2), elevated cardiac enzyme (score 1), hypotension (score 2), tachycardia (score 1), cardiac arrest (score 5), and Killip class III-IV (score 3).
Mapping the Sustainability of Geographical Indication Products: A Systematic Literature Review
Authors: Nirosha R , Mansingh J Paul 
Published: February 2025

Abstract

This review aims to analyze and visualize the significant contribution of geographical indications (GIs) tagged products to sustainability. The research articles included in this study were sourced from the Scopus and the Web of Science databases, and the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) framework was applied to the systematic review of the quality articles. The study conducts comprehensive scientific Bibliometric and TCCM (Theory, Context, Characteristics, and Methodology) analysis over time. The main contribution of these studies is providing opportunities for the development of local economies, cultural heritage preservation, and promoting sustainable agricultural practices through biodiversity conservation, contributing to economic, social, and environmental sustainability. A Conceptual framework has been developed that shows how GI registration links to sustainability and contributes to the United Nations Sustainable Development Goals. Furthermore, the Sustainability of Geographical Indication is an emerging field; there is a need for more research in this area. Some insightful future research directions have been proposed to advance this research domain further, especially in contributing to the UN SDG goals
The application of blended teaching in non-pediatric specialty pediatric science education
Authors: Xue Shu-fang , Zhang Jinyan , Lin Xiang-xiang , Chen Ahong , Lian Ying-jing , Jiang Qi-lin , WANG Cheng-Yi 
Published: February 2025

Abstract

Objective Traditional pediatric education often uses a single teaching method, making theoretical knowledge abstract and difficult to grasp. This can lead to passive learning and limit students' understanding and research skills, failing to prepare them for modern challenges. We studied how blended teaching focused on job competency affects non-specialized pediatric science students.
Methods A total of 133 students in our 2019 class on prevention medicine received blended teaching (reform group), and 143 students in the 2018 class on prevention medicine received traditional teaching (control group). Students records were analyzed retrospectively. Students also evaluated their teachers via questionnaires.
Results Over 60% of students expressed satisfaction with blended learning across three metrics: overall satisfaction, preference for blended learning, and willingness to recommend the course and instructors to others.In the assessments, more than 75% of students in the reform group expressed satisfaction with their grades, which fell within the 95 to 100 range. In contrast, the majority of students in the control group scored between 91 to 95, constituting 34.2% of the sample. Following this, 18.9% achieved grades ranging from 96 to 100, and 17.5% attained scores between 86 to 90. Statistically significant differences were observed between the reform group and the control group in terms of student evaluations, exam scores, case writing and in-class quizzes.
Conclusion We integrated preschool tutoring with classroom education in a non-specialized pediatric medicine course, finding it enhances learning, job readiness, academic performance, and student satisfaction. Tailor the approach to subjects, workload, and performance for best results.
A ferroptosis-related lncRNAs signature as a potential biomarker for diffuse large B-cell lymphoma patients
Authors: Zhang Haoyue , Wang Yuanwen , Zhang Xin , Shao Yanping , Hu Minli 
Published: February 2025

Abstract

Background: Diffuse large B-cell lymphoma (DLBCL) is the most common non-Hodgkin lymphoma subtype in adult patients, with an annual incidence rate ranging from 25.0% to 40.0% worldwide. Nevertheless, the prognosis forthe disease remains poor.
Objective: There is a pressing need for new, reliable biomarkers for prognosis prediction.
Methods: Using 449 DLBCL samples from the Gene Expression Omnibus dataset, the relationships between ferroptosis-associated long non-coding RNAs (lncRNAs) were examined. Before applying univariate Cox analysis to exclude lncRNAs connected to prognosis, we used Pearson correlation analysis to filter a large number of lncRNAs associated with ferroptosis.
Results: To predict the prognosis of DLBCL, eleven lncRNAs linked toferroptosis were subjected to selection operator Cox regression and least absolute shrinkage. Furthermore, it was demonstrated that six ferroptosis-related lncRNAs were the most effective in establishing a predictive risk model. People with DLBCL were assigned to high- and low-risk groups in terms of their median risk scores. The model built employing 11 ferroptosis-related lncRNAs demonstrated higher prognostic evaluation abilities, as demonstrated by the stratified analysis. Significant enrichment in tumor-related pathways was seen in high-risk patients. Age, World Health Organization grade, and the ferroptosis-related lncRNA prognostic factor were taken into consideration when creating a nomogram.
Conclusion: In conclusion, the nomogram generated can precisely anticipate the overall survival of DLBCL patients across both cohorts.
Leveraging Illumina iSeq100 for Whole Genome Sequencing of Salmonella Typhi: a practical approach for resource-limited setting
Authors: Katuwal Nishan , Naga Shiva Ram , Shrestha Aastha , Shahi Sabin Bikram , Tamrakar Dipesh , Shrestha Rajeev 
Published: February 2025

Abstract

Bacterial whole-genome-sequencing helps to improve our understanding of epidemiology and pathogenesis of infections and allows comprehensive investigation on virulence, evolution and resistance mechanisms. Nepal, recently, has seen some increase in sequencing capabilities but faces hurdles for optimum utilisation. However, these hurdles could be alleviated using Illumina iSeq100. Therefore, this study aimed at performing whole-genome-sequencing of bacteria utilising iSeq100. Six banked isolates of S Typhi bacterial were selected, extracted for DNA, confirmed by qPCR and then sequenced in Illumina iSeq100 at 200pM. The consensus was generated by reference-based assembly, mapping onto S. Typhi CT18. These consensus genomes and coverage parameters were compared to data from HiSeq and NextSeq. The raw reads were also evaluated using pathogenwatch to observe genotype, mutations and resistance genes. The coverage parameters (coverage width and depth) of the genomes from this study were compared to same genomes sequenced using HiSeq/NextSeq. The average coverage width (96.81%) and depth (63.75x) of genomes sequenced in iSeq100 were comparable to that of HiSeq/NextSeq (width: 98.72% and depth: 69.87x). The genotypes detected, number of SNPs and genetic determinants of AMR genes were identical. The data from bacterial whole-genome-sequencing using the Illumina iSeq100 is equally informative when compared to some high-end sequencers. Thus, the primary goal of this study is to advocate for optimum utlisation of iSeq100, still ensuring for a high-quality data.