bluuebunny/crossref_metadata_2025_split huggingface dataset:
Penerapan Model Pembelajaran Make a Match Berbantuan Picture Card untuk Meningkatkan Hasil Belajar PPKn Kelas IV
Authors: Farida Selamet , Zuliana Siti , Fakhru Ahsani Eva Luthfi 
Published: May 2024

Penelitian ini bertujuan untuk mengetahui penerapan model pembelajaran make a match dengan menggunakan picture card (kartu bergambar) dalam meningkatkan hasil belajar Pendidikan Pancasila dan Kewarganegaraan materi “Membangun Jati Diri dalam Kebhinekaan” pada siswa kelas IV SD 01 Temulus Mejobo Kudus. Penelitian ini menggunakan metode Penelitian Tindakan Kelas (PTK) yang dilaksanakan dengan dua siklus pada mata pelajaran Pendidikan Pancasila dan Kewarganegaraan dengan subyek siswa kelas IV. Data dianalisis menggunakan teknik penilaian rata-rata hasil belajar dan persentase ketuntasan belajar. Hasil penelitian menunjukkan bahwa rata-rata nilai hasil belajar pada siklus I adalah 73,33 dan meningkat menjadi 82,22 pada siklus II. Selanjutnya persentase ketuntasan belajar siswa pada siklus I memperoleh persentase 77,8%, meningkat menjadi 88,9% pada siklus II.
Методичні положення обґрунтування складу організаційних структур
Authors: Загорка Олексій , Поліщук Сергій , Загорка Ірина , Фреган Наталія 
Published: May 2024

Під час створення або розвитку організації ключовим аспектом є визначення її потрібного складу, тобто сукупності елементів, здатних виконати визначені завдання з максимальною ефективністю за раціонального використання ресурсів. Проте функціонування організації здійснюється в умовах невизначеності. Це ускладнює обґрунтування складу її організаційної структури. Метою статті є розроблення методичних положень обґрунтування складу організаційної структури окресленого типу на основі принципів системного аналізу для виконання завдань за призначенням. Під час проведення дослідження застосовано: метод безпосереднього оцінювання – для визначення експертами ступеня взаємозв’язку згрупованих за визначеними ознаками завдань під час визначення необхідних типів підрозділів (елементів) організаційної структури; метод аналізу ієрархій – для оцінювання важливості завдань, які повинні виконуватися організацією; метод планування експерименту – для формування варіантів складу підрозділів-виконавців завдань; експертний метод ранжирування – для оцінювання важливості показників, що характеризують створення і функціонування організації; метод таксономії – для визначення раціонального варіанта складу організаційної структури. Застосування зазначених методів дало змогу забезпечити визначення складу організаційної структури відповідно до цілей діяльності організації та завдань, що мають виконуватися для їх досягнення, а також необхідних типів підрозділів (елементів) організаційної структури із врахуванням ступеня взаємозв’язку згрупованих завдань. Науковою новизною розроблених методичних положень є сумісне застосування методів планування експериментів і таксономії, що дає можливість визначити збалансований склад підрозділів за ефективністю і вартістю для виконання завдань організації. Особливістю розроблених методичних положень є комплексне застосування вказаних вище методів, що дасть змогу досягнути поставлену мету, яка визначає теоретичну значущість розроблених методичних положень. Розроблені у статті методичні положення можуть використовуватися органами державного і військового управління для обґрунтування збалансованого складу організаційних структур, що створюються або удосконалюються, за ефективністю і часом виконання завдань підрозділами та витратами на їх створення і застосування. Це визначає практичну значущість розроблених методичних положень. Застосування означених положень показано на прикладі.
أنماط الشخصية المضطربة لدى لاعبي كرة الطائرة في الأندية الرياضية في أقليم كوردستان
Authors: Prof. Dr. Ahmed Qasim Muhammad  , Assis  prof .Dr. dunia Sabah Ali  
Published: May 2024

هدفت الدراسة الى التعرف على الأنماط الشائعة للشخصية المضطربة عند لاعبي كرة الطائرة، و التعرف دلالة الفروق في أنماط الشخصية المضطربة عند لاعبو كـرة الطائرة في الأندية الرياضية بإقليم كوردتسان- العراق تبعاً للمتغيرات الآتية: (العمر، مؤهل الدراسي، العمر التدريبي، النوع الاجتماعي، المحافظة). وتتكون العينة من (280) لاعباً ولاعبة. وبواقع (210)لاعباً و (70) لاعبة من الممارسين للعبة كرة الطائرة موزعين على (20) نادياً في اقليم كوردستان العراق، و تتضمن العينة نسبة (52.6%) من مجتمع البحث. وتم تبني مقياس (رحال، 2011) المكون من (90) فقرة موزعة على (9) أبعاد وهي أنماط الشخصية المضطربة: الهسترية،البارانوية، الاكتئابية، المضاد للمجتمع، التجنبية، الحدية، النرجسية، الوسواسية، الاعتمادية ) ، وأمام كل فقرة ذات خمسة بدائل للإجابة. وتمت معالجة البيانات إحصائياً باستعمال الحقيبة الإحصائية للعلوم الاجتماعية (SPSS) ،فأظهرت النتائج الآتي: انخفاض مستوى هذه الأنماط المضطربة من الشخصية عند عينة البحث ككل ،وجود فرق إحصائياً دال عند مستوى دلالة (0.05) لمتغير العمر في أربع أنماط من تلك الأنماط هي الهستيرية، والبارانوية، والوسواسية، والاعتمادية، و وجود فرق دال إحصائياً عند مستوى دلالة (0.05) يعزى لمتغير المؤهل الدراسي في نمط واحد من تلك الأنماط وهي نمط النرجسية لصالح خريجي المعاهد توجد دال فرق إحصائياً لدى دلالة مستوى (0.05) لمتغير الجنس والفروق كان لمصلحة مجموعة جنس الذكور.
Pelatihan Penggunaan Aplikasi Padlet untuk Penunjang Pembelajaran Interaktif dan Komunikatif Era Merdeka Belajar pada Sekolah Penggerak SDN 149 Seluma
Authors: Bogy Restu Ilahi  , Adif Jawadi Saputra  , Andes Permadi  
Published: May 2024

Kegiatan ini berjudul Pelatihan Penggunaan Aplikasi Padlet Untuk Penunjang Pembelajaran Interaktif dan Komunikatif Era Merdeka Belajar Pada Sekolah Penggerak SDN 149 Seluma. Tujuan pengabdian ini untuk untuk menambah pengetahuan dan keterampilan mengajar pada tenaga pengajar di Sekolah Penggerak SDN 149 Seluma tentang Peningkatan Kompetensi dan Literasi Teknologi dalam pembelajaran dengan Metode yang kami gunakan dalam kegiatan pengabdian masyarakat ini adalah metode pelatihan dengan cara ceramah dan tanya jawab serta mengadakan praktek langsung dilanjutkan simulasi bagi para peserta pelatihan. Kegiatan Pelatihan Penggunaan Ipteks berupa Aplikasi Padlet Untuk Untuk Penunjang Pembelajaran Interaktif dan Komunikatif Era Merdeka Belajar Pada Sekolah Penggerak yaitu SDN 149 Seluma. Peserta kegiatan ini adalah Tenaga Pendidik Pada Sekolah Penggerak yaitu SDN 149 Seluma berjumlah 14 orang. Dari pelaksanaan kegiatan tersebut diharapkan bisa menjawab permasalahan yang ada, dimana manfaat yang diharapkan setelah mengikuti pelatihan adalah; (1) Terampil menggunakan Aplikasi Padlet yang sesuai dengan kebutuhan proses belajar mengajar, (2) Meningkatkan pengetahuan guru mengenai pemanfaatan teknologi untuk menunjang pembelajaran yang interaktif dan komunikatif, (3) Meningkatkan kemampuan dan keterampilan guru dalam hal mempersiapkan perangkat pembelajaran yang inovatif, dan (4) Membantu guru untuk menghasilkan media pembelajaran yang berkualitas melalui Aplikasi Padlet.
Pengaruh Senam Kaki dan Pemberian Aromaterapi terhadap Penurunan Glukosa pada Lansia Penderita Diabetes Melitus
Authors: Darma Argi Syahdila , Maryatun Maryatun 
Published: May 2024

Diabetes Melitus adalah suatu penyakit metabolik yang ditandai dengan adanya hiperglikemia yang terjadi karena pankreas tidak mampu mensekresi insulin, gangguan kerja insulin, ataupun keduanya. Diabetes Melitus juga disebut sebagai penyakit kronis yang dapat menyebabkan kerusakan jangka panjang dan kegagalan pada berbagai organ. Mengidentifikasi kadar glukosa darah pada lansia penderita Diabetes Melitus sebelum serta sesudah diberikan terapi senam kaki dan aromaterapi di UPT Puskesmas Ngemplak Kabupaten Boyolali. Tujuan penelitian ini yaitu untuk Mengetahui Pengaruh Senam Kaki dan Pemberian Aromaterapi Terhadap Penurunan Glukosa Pada Lansia Penderita Diabetes Melitus Di UPT Puskesmas Ngemplak Kabupaten Boyolali. Penelitian yang digunakan adalah kuantitatif dengan desain penelitian yang digunakan experimental designs. Hasil penelitian menunjukkan bahwa terapi senam kaki dan pemberian aroma terapi efektif terhadap penurunan glukosa darah didapatkan hasil uji wilcoxon dengan nilai sig 0.00 yang artinya nilai sig < 0.05 maka Ha diterima. Kesimpulan pada penelitian ini yaitu ada hubungan terapi senam kaki dan pemberian aromaterapi dengan penurunan glukosa darah pada penderita Diabetes Melitus Di UPT Puskesmas Ngemplak Kabupaten Boyolali.
Metodología STEAM. Aplicaciones en la educación básica
Authors: Ortiz-Carranza Gabriel , Ortiz-Barre Jaime , Trejo-Márquez Galo , Martínez-Satizabal Edison 
Published: May 2024

STEAM se ha convertido en una revolucionaria técnica que promueve los aprendizajes competentes en el estudiantado. El objetivo de estudio consistió en determinar las aplicaciones de este método que los docentes de educación básica utilizan con los alumnos de una escuela rural.
Como metodología se empleó un enfoque mixto, diseño no experimental de nivel descriptivo, mediante la realización de un cuestionario de encuesta a una población de 58 profesores de la unidad educativa fiscal Chamanga de la provincia de Esmeraldas, Ecuador, así como la entrevista a una experta en esta novedosa instrucción, que permitió fundamentar la discusión de los resultados. Estos revelan que el profesorado apenas conoce de este importante recurso, aunque una mayoría del 86% está dispuesto a utilizarlo con los alumnos para enfrentar problemáticas reales y encontrar soluciones creativas, empero ya realizan algunas actividades relacionadas con esta herramienta, aunque no de forma transdisciplinar; un 38% espera beneficios como el incentivo a explorar nuevos conceptos y experimentar con diferentes soluciones, mientras un 28% espera poder estimular el pensamiento crítico y promover estrategias de resolución.
Se concluye en que, hace falta que el profesorado pueda instruirse en la metodología STEAM para impartir los conocimientos enfocados a adquirir competencias, guiando en todo el proceso a los escolares, haciendo el mejor uso de los tiempos, generando un ambiente de aprendizaje positivo y agradable, motivándolos desde la base de lo que van aprendiendo, y evaluándolos cuantitativamente cuando hayan obtenido logros positivos, que a su vez, continúen promoviéndolos a nuevos logros.
STUDI KEANEKARAGAMAN MIKROALGA DI SUNGAI MARGASATWA RAGUNAN SEKITAR KANDANG BUAYA
Authors: Setyani Anisa Intan , Sari Deva Dewiyana , Permatasari Maulida Azzra , Izat Muhammad , Bintang Sekar , Suryanda Ade , Azrai Eka Putri 
Published: May 2024

Microalgae are microscopic unicellular organisms that have varying sizes, shapes, and types, have photosynthetic pigments, are photoautotrophic, and are found in aquatic environments. Microalgae have certain limits of tolerance to water chemical physics factors. The presence of microalgae in water can be used as an indicator of the state of the water. This study aims to identify the diversity of microalgae as an indicator of the water quality present in the river around the Ragunan Margasatwa crocodile cage. Sampling was done in the Ragunan Margasatwa Park river near the crocodile cage. Samples of macroalgae are visually identified and include morphology, color, shape, and other characteristics. Based on the observations, it can be concluded that the river ecosystems around the crocodile cages are under low ecosystem pressure. The diversity index generally has a range of values between 1.5 and 4, and lower values reflect increasing ecosystem instability. The lowest microalgae density was found at station 1, which is 120 ind/ml. Individuals with the highest density at station 2, the species Aphanothece sp. with a density value of 293.3 ind/mL.
Analysis of risk factors for the recurrence of osteomyelitis of the limb after treatment with antibiotic-loaded calcium sulfate and autologous bone graft
Authors: Su Yu , Li Dongchen , Du Bing , Li Zhao , Lu Yao , Xu Yibo , Wang Qian , Li Zhong , Ren Cheng , Ma Teng 
Published: May 2024

ObjectiveWe aimed to evaluate the efficacy of antibiotic-loaded calcium sulfate combined with autologous iliac bone transplantation in the treatment of limb-localized osteomyelitis (Cierny–Mader type III) and analyze the causes and risk factors associated with infection recurrence.MethodsClinical data of 163 patients with localized osteomyelitis of the extremities treated with antibiotic-loaded calcium sulfate combined with autologous iliac bone transplantation in Xi’an Honghui Hospital from January 2017 to December 2022 were retrospectively analyzed. All patients were diagnosed with localized osteomyelitis through clinical examination and treated with antibiotic-loaded calcium sulfate combined with autologous iliac bone. Based on the infection recurrence status, the patients were divided into the recurrence group and the non-recurrence group. The clinical data of the two groups were compared using univariate analysis. Subsequently, the distinct datasets were included in the binary logistic regression analysis to determine the risk and protective factors.ResultsThis study included 163 eligible patients, with an average age of 51.0 years (standard deviation: 14.9). After 12 months of follow-up, 25 patients (15.3%) experienced infection recurrence and were included in the recurrence group; the remaining 138 patients were included in the non-recurrence group. Among the 25 patients with recurrent infection, 20 required reoperation, four received antibiotic treatment alone, and one refused further treatment. Univariate analysis showed that education level, smoking, hypoproteinemia, open injury-related infection, and combined flap surgery were associated with infection recurrence (p &lt; 0.05). Logistic regression analysis showed that open injury-related infection (odds ratio [OR] = 35.698; 95% confidence interval [CI]: 5.997–212.495; p &lt; 0.001) and combined flap surgery (OR = 41.408; 95% CI: 5.806–295.343; p &lt; 0.001) were independent risk factors for infection recurrence. Meanwhile, high education level (OR = 0.009; 95% CI: 0.001–0.061; p &lt; 0.001) was a protective factor for infection recurrence.ConclusionAntibiotic-loaded calcium sulfate combined with autologous iliac bone transplantation is an effective method for treating limb-localized osteomyelitis. Patients without previous combined flap surgery and non-open injury-related infections have a relatively low probability of recurrence of infection after treatment with this surgical method. Additionally, patients with a history of smoking and hypoproteinemia should pay attention to preventing the recurrence of infection after operation. Providing additional guidance and support, particularly in patients with lower education levels and compliance, could contribute to the reduction of infection recurrence.
Enhancing diabetes therapy adherence: a comprehensive study on glucometer usability for type 2 diabetes patients
Authors: Toletti Giovanni , Boaretto Andrea , Di Loreto Chiara , Fornengo Riccardo , Gigante Alfonso , Perrone Giovanni 
Published: May 2024

BackgroundSelf-monitoring of blood glucose (SMBG) is a vital practice for type 2 diabetes (T2DM), and glucometers have the potential to improve therapy adherence. However, characteristics of glucometers improving their usability are underexplored. A knowledge gap exists regarding patients under 65, warranting further research for diabetes care improvement. Thus, this study aims to gather insights on glucometer accessibility, by analyzing the case of the Accu-Chek® Instant glucometer by Roche Diabetes Care GmbH.MethodsStarting from a previous study having the objective of investigating devices’ features able to improve SMBG in over 65 T2DM patients, using the same device, we enlarged the scale, designing a survey that collected answers from 1145 patients of the Center and South of Italy, both under and over 65. 957 answers were analyzed, according to a threshold of 50% completion of the answers.ResultsOur results show the major characteristics presented in Accu-Chek® Instant are appreciated differently between patients under 65 and over 65, and between patients with or without previous experience with a glucometer.Discussions and conclusionsIt emerged how Accu-Chek® was perceived as more user-friendly among individuals under 65 compared to those aged 65 and over, where more people had prior experience, indicating how such a glucometer can be particularly helpful for naive patients. The study provides valuable insights to the academic discourse on glucometer features and their influence on therapy adherence.
Spatial detection of mitochondrial DNA and RNA in tissues
Authors: Giarmarco Michelle , Seto Jordan , Brock Daniel , Brockerhoff Susan 
Published: May 2024

BackgroundMitochondrial health has gained attention in a number of diseases, both as an indicator of disease state and as a potential therapeutic target. The quality and amount of mitochondrial DNA (mtDNA) and RNA (mtRNA) can be important indicators of mitochondrial and cell health, but are difficult to measure in complex tissues.MethodsmtDNA and mtRNA in zebrafish retina samples were fluorescently labeled using RNAscope™ in situ hybridization, then mitochondria were stained using immunohistochemistry. Pretreatment with RNase was used for validation. Confocal images were collected and analyzed, and relative amounts of mtDNA and mtRNA were reported. Findings regarding mtDNA were confirmed using qPCR.ResultsSignals from probes detecting mtDNA and mtRNA were localized to mitochondria, and were differentially sensitive to RNase. This labeling strategy allows for quantification of relative mtDNA and mtRNA levels in individual cells. As a demonstration of the method in a complex tissue, single photoreceptors in zebrafish retina were analyzed for mtDNA and mtRNA content. An increase in mtRNA but not mtDNA coincides with proliferation of mitochondria at night in cones. A similar trend was measured in rods.DiscussionMitochondrial gene expression is an important component of cell adaptations to disease, stress, or aging. This method enables the study of mtDNA and mtRNA in single cells of an intact, complex tissue. The protocol presented here uses commercially-available tools, and is adaptable to a range of species and tissue types.
Cytosolic DNA sensors activation of human astrocytes inhibits herpes simplex virus through IRF1 induction
Authors: Liu Yu , Xu Xi-Qiu , Li Wei-Jing , Zhang Biao , Meng Feng-Zhen , Wang Xu , Majid Safah M. , Guo Zihan , Ho Wen-Zhe 
Published: May 2024

IntroductionWhile astrocytes participate in the CNS innate immunity against herpes simplex virus type 1 (HSV-1) infection, they are the major target for the virus. Therefore, it is of importance to understand the interplay between the astrocyte-mediated immunity and HSV-1 infection.MethodsBoth primary human astrocytes and the astrocyte line (U373) were used in this study. RT-qPCR and Western blot assay were used to measure IFNs, the antiviral IFN-stimulated genes (ISGs), IFN regulatory factors (IRFs) and HSV-1 DNA. IRF1 knockout or knockdown was performed with CRISPR/Cas9 and siRNA transfection techniques.ResultsPoly(dA:dT) could inhibit HSV-1 replication and induce IFN-β/IFN-λs production in human astrocytes. Poly(dA:dT) treatment of astrocytes also induced the expression of the antiviral ISGs (Viperin, ISG56 and MxA). Among IRFs members examined, poly(dA:dT) selectively unregulated IRF1 and IRF9, particularly IRF1 in human astrocytes. The inductive effects of poly(dA:dT) on IFNs and ISGs were diminished in the IRF1 knockout cells. In addition, IRF1 knockout attenuated poly(dA:dT)-mediated HSV-1 inhibition in the cells.ConclusionThe DNA sensors activation induces astrocyte intracellular innate immunity against HSV-1. Therefore, targeting the DNA sensors has potential for immune activation-based HSV-1 therapy.
Correlation between plasma aldosterone concentration and bone mineral density in middle-aged and elderly hypertensive patients: potential impact on osteoporosis and future fracture risk
Authors: Song Shuaiwei , Cai Xintian , Hu Junli , Zhu Qing , Shen Di , Heizhati Mulalibieke , Yang Wenbo , Hong Jing , Li Nanfang 
Published: May 2024

BackgroundPrevious studies have suggested that aldosterone may play a major role in calcium-phosphorus homeostasis and bone metabolism. However, the relationship between plasma aldosterone concentrations (PAC) and bone mineral density (BMD) in middle-aged and elderly hypertensive patients remains unclear. Therefore, this study sought to investigate the relationship between PAC levels and BMD and explore PAC’s potential impact on osteoporosis and future fracture risk in hypertensive patients.MethodsOur study included a total of 1430 participants. Associations are tested using multiple linear and logistic regression models. Nonlinearity was investigated using the restricted cubic spline (RCS). We also performed mediating analyses to assess mediating factors mediating the relationship between PAC and osteoporosis.ResultsThe multiple linear regression showed a negative correlation between PAC and BMD and was generally positively associated with FRAX scores. Meanwhile, logistic regression analyses indicated that osteoporosis was highly correlated with PAC levels. In addition, a clear non-linear dose-response relationship was also shown in the constructed RCS model. Finally, mediation analyses showed that serum potassium played an important role in the development of osteoporosis.ConclusionThis study demonstrates that elevated PAC levels are strongly associated with decreased BMD, increased prevalence of osteoporosis, and the risk of future fractures in middle-aged and elderly hypertensive patients. Further studies are needed to confirm this relationship and reveal its underlying mechanisms.
Non-cross-reactive epitopes dominate the humoral immune response to COVID-19 vaccination – kinetics of plasma antibodies, plasmablasts and memory B cells
Authors: Wietschel Kilian A. , Fechtner Kevin , Antileo Elmer , Abdurrahman Goran , Drechsler Chiara A. , Makuvise Michelle Kudzayi , Rose Ruben , Voß Mathias , Krumbholz Andi , Michalik Stephan , Weiss Stefan , Ulm Lena , Franikowski Philipp , Fickenscher Helmut , Bröker Barbara M. , Raafat Dina , Holtfreter Silva 
Published: May 2024

IntroductionCOVID-19 vaccines are highly effective in inducing protective immunity. While the serum antibody response to COVID-19 vaccination has been studied in depth, our knowledge of the underlying plasmablast and memory B cell (Bmem) responses is still incomplete. Here, we determined the antibody and B cell response to COVID-19 vaccination in a naïve population and contrasted it with the response to a single influenza vaccination in a primed cohort. In addition, we analyzed the antibody and B cell responses against the four endemic human coronaviruses (HCoVs).MethodsMeasurement of specific plasma IgG antibodies was combined with functional analyses of antibody-secreting plasmablasts and Bmems. SARS-CoV-2- and HCoV-specific IgG antibodies were quantified with an in-house bead-based multiplexed immunoassay.ResultsThe antibody and B cell responses to COVID-19 vaccination reflected the kinetics of a prime-boost immunization, characterized by a slow and moderate primary response and a faster and stronger secondary response. In contrast, the influenza vaccinees possessed robust immune memory for the vaccine antigens prior to vaccination, and the recall vaccination moderately boosted antibody production and Bmem responses. Antibody levels and Bmem responses waned several months after the 2nd COVID-19 vaccination, but were restored upon the 3rd vaccination. The COVID-19 vaccine-induced antibodies mainly targeted novel, non-cross-reactive S1 epitopes of the viral spike protein, while cross-reactive S2 epitopes were less immunogenic. Booster vaccination not only strongly enhanced neutralizing antibodies against an original SARS-CoV-2 strain, but also induced neutralizing antibodies against the Omicron BA.2 variant. We observed a 100% plasma antibody prevalence against the S1 subunits of HCoVs, which was not affected by vaccination.DiscussionOverall, by complementing classical serology with a functional evaluation of plasmablasts and memory B cells we provide new insights into the specificity of COVID-19 vaccine-induced antibody and B cell responses.