bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cellular distribution of Glut‐1 and Glut‐5 in benign and malignant human prostate tissue
Authors: Reinicke Karin , Sotomayor Paula , Cisterna Pedro , Delgado Carolina , Nualart Francisco , Godoy Alejandro 
Published: September 2011

AbstractOver‐expression of hexose transporters (Gluts), specifically Glut‐1, is a common event in human malignancies. In prostate cancer (CaP), however, expression of Gluts has been characterized poorly. In this study, expression and distribution of Glut‐1 and Glut‐5 proteins were characterized using immunohistochemistry in 76 specimens of benign prostate, 10 specimens of high‐grade intraepithelial neoplasia (HGPIN), and 28 specimens of CaP. In addition, mRNA expression of Glut‐2, Glut‐7, Glut‐9, and Glut‐11 was analyzed in a set of five specimens of benign prostate and CaP. In benign prostate, Glut‐1 localized to the basal cells and to the basolateral membrane of secretory/luminal epithelial cells. Glut‐5, however, localized to the apical membrane of secretory/luminal epithelial cells. In HGPIN, Glut‐1 was immunohistochemically undetectable. Glut‐5, however, localized to the apical membrane of the neoplastic epithelial cells. In CaP, Glut‐1 and Glut‐5, were immunohistochemically undetectable. However, over‐expression of GLUT1 was observed in some specimens of highly proliferative intraductal CaP. Glut‐7, Glut‐9, and Glut‐11 mRNAs were detected in benign prostate and CaP, however, only Glut‐11 mRNA was consistently up‐regulated in CaP compared to benign prostate. Low levels of expression of Glut‐1 protein in the majority of CaP could explain, at least in part, the limited clinical applicability of positron emission tomography using 2‐[18F]‐fluoro‐2‐deoxy‐D‐glucose for imaging CaP. Moreover, expression of Glut‐5 in HGPIN suggested that fructose could be utilized as potential metabolic substrate in HGPIN. Understanding the molecular mechanisms involved in regulation/dysregulation of Gluts in CaP could provide insight in the understanding of hexose metabolism in CaP. J. Cell. Biochem. 113: 553–562, 2012. © 2011 Wiley Periodicals, Inc.
Improving protein structural class prediction using novel combined sequence information and predicted secondary structural features
Authors: Dai Qi , Wu Li , Li Lihua 
Published: September 2011

AbstractProtein structural class prediction solely from protein sequences is a challenging problem in bioinformatics. Numerous efficient methods have been proposed for protein structural class prediction, but challenges remain. Using novel combined sequence information coupled with predicted secondary structural features (PSSF), we proposed a novel scheme to improve prediction of protein structural classes. Given an amino acid sequence, we first transformed it into a reduced amino acid sequence and calculated its word frequencies and word position features to combine novel sequence information. Then we added the PSSF to the combine sequence information to predict protein structural classes. The proposed method was tested on four benchmark datasets in low homology and achieved the overall prediction accuracies of 83.1%, 87.0%, 94.5%, and 85.2%, respectively. The comparison with existing methods demonstrates that the overall improvements range from 2.3% to 27.5%, which indicates that the proposed method is more efficient, especially for low‐homology amino acid sequences. © 2011 Wiley Periodicals, Inc. J Comput Chem, 2011
Tight‐binding molecular dynamics simulation of ZnSe liquid within the local environment dependence
Authors: Laref A. 
Published: September 2011

AbstractWe investigate the structural, electronic and dynamical properties of ZnSe liquid using tight‐binding molecular dynamics (TBMD) simulations. We report the TBMD calculations for the solid and liquid forms of the ZnSe compound. To produce more realistic results the TB model includes the local environment dependence in the Hamiltonian matrix at finite temperature for ZnSe. To further demonstrate the efficiency of the TBMD approach, we present results for finite temperature physical properties of ZnSe liquid. We are able to show good agreement with experiment for the atomic mean‐squared displacement and melting point. © 2011 Wiley Periodicals, Inc. J Comput Chem, 2011
Nonadiabatic quantum dynamics in O(3P)+H2→OH+H: A revisited study
Authors: Han Boran , Zheng Yujun 
Published: September 2011

AbstractTo investigate the extent of nonadiabatic effects in the title reaction, quasi‐classical trajectory and nonadiabatic quantum scattering as well as the nonadiabatic quantum‐classical trajectory calculations were performed on the accurate ab initio benchmark potential energy surfaces of the lowest 3A′ and 3A″ electronic states [Rogers et al., J Phys Chem A 2000, 104, 2308], together with the spin‐orbit coupling matrix [Maiti and Schatz, J Chem Phys 2003, 119, 12360] and the lowest singlet 1A′ potential energy surface [Dobby and Knowles, Faraday Discuss 1998, 110, 247]. Comparison of the calculated total cross sections from both adiabatic and nonadiabatic calculations has demonstrated that for adiabatic channels including 3A′→3A′ and 3A″→3A″, difference does exist between the two kinds of adiabatic and nonadiabatic calculations, showing nonadiabatic effects to some extent. Such nonadiabatic effects tend to become more conspicuous at high collision energies and are found to be more pronounced with trajectories/quantum wave packet initiated on 3A′ than on 3A″. Furthermore, the present study also showed that nonadiabatic effects can bring the component of forward‐scattering in the product angular distributions. © 2011 Wiley Periodicals, Inc. J Comput Chem, 2011
AMIGO is expressed in multiple brain cell types and may regulate dendritic growth and neuronal survival
Authors: Chen Yanan , Hor Hong Huan , Tang Bor Luen 
Published: September 2011

AbstractAmphoterin‐induced gene and ORF (AMIGO) is a brain‐enriched transmembrane immunoglobulin (Ig) superfamily protein with six extracellular leucine‐rich repeats (LRR) and a single immunoglobulin‐like (Ig) domain. We report here that AMIGO is a glycosylated protein widely expressed in the central nervous system (CNS), and can be found in neurons, astrocytes as well as oligodendrocytes. In morphologically mature primary neurons, endogenous AMIGO, and transfected full length AMIGO (AMIGO‐FL) are largely dendritic, while AMIGO with its LRR domain deleted (AMIGO‐Ig) is predominantly axonal. In line with AMIGO's dendritic localization, siRNA‐mediated silencing of AMIGO resulted in reduced dendritic growth of cortical neurons in culture. SH‐SY5Y cells stably over‐expressing AMIGO are more resistant to apoptosis induced by staurosporine and H2O2 compared to vector controls. AMIGO therefore likely plays important roles in dendritic outgrowth during development, and could modulate the survival of developing and adult neurons. J. Cell. Physiol. 227: 2217–2229, 2012. © 2011 Wiley Periodicals, Inc.
Imatinib treatment inhibit IL‐6, IL‐8, NF‐KB and AP‐1 production and modulate intracellular calcium in CML patients
Authors: Ciarcia Roberto , Vitiello Maria Teresa , Galdiero Massimiliano , Pacilio Carmen , Iovane Valentina , d'Angelo Danila , Pagnini David , Caparrotti Giuseppe , Conti Daniele , Tomei Valentina , Florio Salvatore , Giordano Antonio 
Published: September 2011

AbstractImatinib (IM) is considered the gold standard for chronic myeloid leukemia (CML) treatment, although resistance is emerging as a significant problem. The proinflammatory cytokines interleukin‐6 (IL‐6) and interleukin‐8 (IL‐8) play an important role in cell proliferation, survival, and resistance to glucocorticoid‐mediated cell death. Several transcription factors such as NF‐KB and AP‐1 are activated in response to physiopathological increases and modulation of intracellular calcium levels. Our previous study demonstrated that lymphocytes from CML patients showed dysregulated calcium homeostasis and oxidative stress. Alteration in ionized calcium concentration in the cytosol has been implicated in the initiation of secretion, contraction, and cell proliferation. In this study, we hypothesized that IL‐6, IL‐8, NF‐kB, AP‐1, and intracellular calcium may be used as selective and prognostic factors to address the follow‐up in CML patients treated with imatinib. Our results demonstrated a significant down‐regulation in IL‐6 and IL‐8 release as well as NF‐kB and AP‐1 activation in lymphomonocytes from Imatinib‐treated patients, compared to samples from untreated patients. In parallel, IM treatment, in vivo and in vitro, were able to modulate the intracellular calcium concentration of peripheral blood mononuclear cells of CML patients by acting at the level of InsP3 receptor in the endoplasmic reticulum and at the level of the purinergic receptors on plasma membrane. The results of this study show that measurements of NF‐kB, AP‐1, IL‐6, IL‐8, and intracellular calcium in CML patients treated with Imatinib may give important information to the hematologist on diagnostic criteria and are highly predictive in patients with newly diagnosed CML. J. Cell. Physiol. 227: 2798–2803, 2012. © 2011 Wiley Periodicals, Inc.
Endurance exercise training increases APPL1 expression and improves insulin signaling in the hepatic tissue of diet‐induced obese mice, independently of weight loss
Authors: Marinho R. , Ropelle E.R. , Cintra D.E. , De Souza C.T. , Da Silva A.S.R. , Bertoli F.C. , Colantonio E. , D'Almeida V. , Pauli J.R. 
Published: September 2011

AbstractHepatic insulin resistance is the major contributor to fasting hyperglycemia in type 2 diabetes. The protein kinase Akt plays a central role in the suppression of gluconeogenesis involving forkhead box O1 (Foxo1) and peroxisome proliferator‐activated receptor gamma co‐activator 1 alpha (PGC‐1α), and in the control of glycogen synthesis involving the glycogen synthase kinase beta (GSK3β) in the liver. It has been demonstrated that endosomal adaptor protein APPL1 interacts with Akt and blocks the association of Akt with its endogenous inhibitor, tribbles‐related protein 3 (TRB3), improving the action of insulin in the liver. Here, we demonstrated that chronic exercise increased the basal levels and insulin‐induced Akt serine phosphorylation in the liver of diet‐induced obese mice. Endurance training was able to increase APPL1 expression and the interaction between APPL1 and Akt. Conversely, training reduced both TRB3 expression and TRB3 and Akt association. The positive effects of exercise on insulin action are reinforced by our findings that showed that trained mice presented an increase in Foxo1 phosphorylation and Foxo1/PGC‐1α association, which was accompanied by a reduction in gluconeogenic gene expressions (PEPCK and G6Pase). Finally, exercised animals demonstrated increased at basal and insulin‐induced GSK3β phosphorylation levels and glycogen content at 24 h after the last session of exercise. Our findings demonstrate that exercise increases insulin action, at least in part, through the enhancement of APPL1 and the reduction of TRB3 expression in the liver of obese mice, independently of weight loss. J. Cell. Physiol. 227: 2917–2926, 2012. © 2011 Wiley Periodicals, Inc.
PI3K: A potential therapeutic target for cancer
Authors: Chen Yingwei , Wang Bao‐Can , Xiao Yongtao 
Published: September 2011

AbstractPhosphatidylinositol 3‐kinase (PI3K), one member of lipid kinase family, has been demonstrated to play a key role in regulating cell proliferation, adhesion, survival, and motility. Recent studies indicate that PI3K related signaling pathway is one of the most commonly activated pathways in human cancers. Accordingly, pharmacological inhibition of key nodes in this signaling cascade has been a focus in developmental therapeutics. To date, Inhibitors targeting PI3K or nodes in this pathway, AKT and mTOR, are best studied and have reached clinical trials. In this review, we will focus on recent progress on understanding of PI3Ks signaling pathway and the development of PI3K inhibitors. J. Cell. Physiol. 227: 2818–2821, 2012. © 2011 Wiley Periodicals, Inc.
Regional brain axial and radial diffusivity changes during development
Authors: Kumar Rajesh , Nguyen Haidang D. , Macey Paul M. , Woo Mary A. , Harper Ronald M. 
Published: September 2011

AbstractThe developing human brain shows rapid myelination and axonal changes during childhood, adolescence, and early adulthood, requiring successive evaluations to determine normative values for potential pathological assessment. Fiber characteristics can be examined by axial and radial diffusivity procedures, which measure water diffusion parallel and perpendicular to axons and show primarily axonal status and myelin changes, respectively. Such measures are lacking from widespread sites for the developing brain. Diffusion tensor imaging data were acquired from 30 healthy subjects (age 17.7 ± 4.6 years, range 8–24 years, body mass index 21.5 ± 4.5 kg/m2, 18 males) using a 3.0‐Tesla MRI scanner. Diffusion tensors were calculated, principal eigenvalues determined, and axial and radial diffusivity maps calculated and normalized to a common space. A set of regions of interest was outlined from widespread brain areas within rostral, thalamic, hypothalamic, cerebellar, and pontine regions, and average diffusivity values were calculated using normalized diffusivity maps and these regions of interest masks. Age‐related changes were assessed with Pearson's correlations, and gender differences evaluated with Student's t‐tests. Axial and radial diffusivity values declined with age in the majority of brain areas, except for midhippocampus, where axial diffusivity values correlated positively with age. Gender differences emerged within putamen, thalamic, hypothalamic, cerebellar, limbic, temporal, and other cortical sites. Documentation of normal axial and radial diffusivity values will help assess disease‐related tissue changes. Axial and radial diffusivities change with age,with fiber structure and organization differing between sexes in several brain areas. The findings may underlie gender‐based functional characteristics, and mandate partitioning age‐ and gender‐related changes during developmental brain pathology evaluation. © 2011 Wiley Periodicals, Inc.
Shock machine for the mechanical behaviour of hip prostheses: a description of performance capabilities
Authors: Uribe Juliana , Geringer Jean , Forest Bernard 
Published: September 2011

ABSTRACTThe aim of this study was to describe the behaviour of a shock machine designed for testing hip prostheses. A microseparation between head and cup occurs inducing a shock of several times the body weight, leading to fracture of ceramic femoral components. Femoral heads and cups of diameter 32 mm manufactured from alumina were tested in dry and wet conditions. Implants were subjected to shocks with a load profile of 9 kN load at 2 Hz and various microseparations. Position is monitored and force is measured with two acquisition systems. The working range and the device capabilities were investigated. Only cups tested in dry conditions failed. Observations by scanning electron microscopy revealed intergranular and transgranular fractures. Two wear stripes were observed on the heads. Three‐dimensional roughness of wear stripes was measured. Since experimental results are in good agreement with retrieved femoral heads, the shocks machine reproduces the in vivo degradations. Copyright © 2011 John Wiley & Sons, Ltd.
Anti‐proliferative effect of curcumin on melanoma cells is mediated by PDE1A inhibition that regulates the epigenetic integrator UHRF1
Authors: Abusnina Abdurazzag , Keravis Thérèse , Yougbaré Issaka , Bronner Christian , Lugnier Claire 
Published: September 2011

AbstractScope: Curcumin inhibits proliferation of many cancer cells. Cyclic nucleotide phosphodiesterases (PDEs), by hydrolyzing intracellular cyclic adenosine‐3′,5′‐monophosphate (cAMP) and/or cyclic guanosine‐3′,5′‐monophosphate (cGMP), play a pivotal role in signalling pathways involved in cell proliferation. Therefore, this study investigated PDE1–5 participations in the anti‐proliferative properties of curcumin in B16F10 murine melanoma cells.Methods and results: We report that curcumin inhibits PDE1–5 activities (IC50≅10−5 M), indicating that curcumin acts as a non‐selective PDE inhibitor. In melanoma cells, PDE4 and PDE1 represent the major cAMP‐PDEs and cGMP‐PDEs activities, respectively. Curcumin treatment decreased PDE1 and PDE4 activities and dose dependently increased intracellular cGMP levels, whereas cAMP levels were unchanged. Curcumin inhibited cell proliferation and cell cycle progression by accumulating cells in the S‐ and G2/M‐phases with enhanced expressions of cyclin‐dependent kinase inhibitors. In contrast, expressions of PDE1A, cyclin A and the epigenetic integrator ubiquitin‐like containing PHD and Ring Finger domains 1 (UHRF1) and DNA methyltransferase 1 (DNMT1) were decreased by curcumin. Interestingly, PDE1A overexpression increased UHRF1 and DNMT1 expressions and rescued the B16F10 cells from curcumin anti‐proliferative effects. Nimodipine, a PDE1 inhibitor, mimicked the curcumin effects. Conclusion: Curcumin exerts its anti‐cancer property by targeting PDE1 that inhibits melanoma cell proliferation via UHRF1, DNMT1, cyclin A, p21 and p27 regulations. This suggests that natural PDE1 inhibitors present in food might be effective in preventing cancer.
Curcumin provides potential protection against the activation of hypoxia and prolyl 4‐hydroxylase inhibitors on prostate‐specific antigen expression in human prostate carcinoma cells
Authors: Chung Li‐Chuan , Tsui Ke‐Hung , Feng Tsui‐Hsia , Lee Shiow‐Ling , Chang Phei‐Lang , Juang Horng‐Heng 
Published: September 2011

AbstractScope: Prostate‐specific antigen (PSA) is a well‐known marker for diagnosing and monitoring prostate cancer. Curcumin, a yellow curry pigment, has been reported to enhance androgen receptor (AR) degradation. We examined the effects of curcumin on increasing PSA expression by hypoxia and prolyl hydroxylase inhibitors, L‐mimosine and dimethyloxalylglycine (DMOG), in human prostate carcinoma LNCaP cells.Methods and results: The 3H‐thymidine incorporation assay revealed that either L‐mimosine or DMOG treatments attenuated cell proliferation. Immunoblot and enzyme‐linked immunosorbent assays (ELISA) indicated that both L‐mimosine and DMOG have an effect similar to hypoxia, which stabilized hypoxia‐inducible factor‐1α (HIF‐1α) and induced PSA gene expression. The results of the immunoblot and transient gene expression assays indicated that induction of the PSA expression by hypoxia is both HIF‐1α‐ and AR‐dependent. Immunoblot assays revealed that a curcumin treatment (10 μM) decreased the protein abundance of AR but did not significantly affect the protein levels of HIF‐1α and vascular endothelial growth factor, which were induced by hypoxia. ELISA and transient gene expression assays indicated that curcumin blocked the activation of L‐mimosine or DMOG treatment on PSA expression.Conclusions: These results indicate that curcumin blocked the enhanced effect of PSA expression by L‐mimosine and DMOG that induce hypoxia condition.
Can the efficacy of electrically stimulated pedaling using a commercially available ergometer BE improved by minimizing the muscle stress–time integral?
Authors: Hakansson Nils A. , Hull Maury L. 
Published: September 2011

AbstractIntroduction: The cardiorespiratory and muscular strength benefits of functional electrical stimulation (FES) pedaling for spinal cord injury (SCI) subjects are limited because the endurance of electrically stimulated muscle is low. Methods: We tested new electrical stimulation timing patterns (Stim3, designed using a forward dynamic simulation to minimize the muscle stress–time integral) to determine whether SCI subjects could increase work and metabolic responses when pedaling a commercial FES ergometer. Work, rate of oxygen uptake (V̇O2), and blood lactate data were taken from 11 subjects (injury level T4–T12) on repeated trials. Results: Subjects performed 11% more work pedaling with Stim3 than with existing stimulation patterns (StimErg) (P = 0.043). Average (V̇O2) and blood lactate concentrations were not significantly different between Stim3 (442 ml/min, 5.9 mmol/L) and StimErg (417 ml/min, 5.9 mmol/L). Conclusion: The increased mechanical work performed with Stim3 supports the use of patterns that minimize the muscle stress–time integral to prolong FES pedaling. Muscle Nerve, 2012
Pharmacovigilance systems and databases in Korea, Japan, and Taiwan
Authors: Kimura Tomomi , Matsushita Yasuyuki , Yang Yea‐Huei Kao , Choi Nam‐Kyong , Park Byung‐Joo 
Published: September 2011

ABSTRACTPurposeThe aim of this article is to qualitatively describe the characteristics of spontaneous reporting systems, signal detection methods, and observational databases in Korea, Japan, and Taiwan.MethodsNational statistics and published literature were reviewed to determine the annual number of spontaneous reports and the main source (reporters) for each country. The data items collected for spontaneous reports, mode of transmission, availability of reports, and quantitative methods for signal detection were compared across the countries. The characteristics of the national insurance claims databases also were summarized.ResultsThe number of annual domestic spontaneous reports in 2007 from Korea, Japan, and Taiwan were 3751, 32 391, and 6971, respectively, which increased to 26 827, 34 649, and 10 320, respectively, over the next 2 years. If adjusted by total population, the latter would be 5.59, 2.71, and 4.49 per 10 000 persons, respectively. The reports were directly submitted mainly by physicians (51%) in Korea and hospital pharmacists (65%) in Taiwan, whereas 89% of the reports in Japan were submitted by manufacturers. Routine data mining of the national spontaneous report database has been implemented in Japan and will be in Korea and Taiwan.ConclusionsDifferences in quantity and submission practice of spontaneous reports should be noted for potential comparison among these countries/regions, as well as with those from Western countries, even after the full implementation of national data mining system. Nationwide claims databases with nearly 100% coverage are great assets for pharmacovigilance and pharmacoepidemiology and can be major contributors to global pharmacovigilance. Copyright © 2011 John Wiley & Sons, Ltd.
Microscopic study of the efficiency of Coulomb‐ and phonon‐induced relaxation channels in graphene
Authors: Winzer Torben , Malić Ermin 
Published: September 2011

AbstractWe present a microscopic approach on ultrafast relaxation dynamics of optically excited carriers in graphene. In agreement with experimental differential transmission spectra, we find the dynamics to be characterized by two timescales: the ultrafast femtosecond thermalization and the carrier cooling on a picosecond timescale. The framework of the density matrix theory allows to separately analyze the strength of different relaxation channels, such as carrier–carrier and carrier–phonon scattering, resolved in time, momentum, and angle. Our calculations reveal that the initial thermalization is governed by the Coulomb interaction, however the carrier–phonon scattering also considerably contributes to the process of forming a hot Fermi distribution. The energy dissipation and the cooling of carriers is determined by phonon‐induced intra‐ and interband scattering.