bluuebunny/crossref_metadata_2025_split huggingface dataset:
The CRZ1 transcription factor in plant fungi: regulation mechanism and impact on pathogenesis
Authors: Cacciotti A. , Beccaccioli M. , Reverberi M. 
Published: May 2024

Abstract
Calcium (Ca2+) is a universal signaling molecule that is tightly regulated, and a fleeting elevation in cytosolic concentration triggers a signal cascade within the cell, which is crucial for several processes such as growth, tolerance to stress conditions, and virulence in fungi. The link between calcium and calcium-dependent gene regulation in cells relies on the transcription factor Calcineurin-Responsive Zinc finger 1 (CRZ1). The direct regulation of approximately 300 genes in different stress pathways makes it a hot topic in host-pathogen interactions. Notably, CRZ1 can modulate several pathways and orchestrate cellular responses to different types of environmental insults such as osmotic stress, oxidative stress, and membrane disruptors. It is our belief that CRZ1 provides the means for tightly modulating and synchronizing several pathways allowing pathogenic fungi to install into the apoplast and eventually penetrate plant cells (i.e., ROS, antimicrobials, and quick pH variation). This review discusses the structure, function, regulation of CRZ1 in fungal physiology and its role in plant pathogen virulence.
Investigating practice integration of independent prescribing by community pharmacists using normalization process theory: a cross-sectional survey
Authors: Karim L. , McIntosh T. , Jebara T. , Pfleger D. , Osprey A. , Cunningham S. 
Published: May 2024

Abstract
Background
Independent prescribing (IP) has not been extensively investigated in community pharmacy (CP). Normalization process theory (NPT) constructs help explain how interventions are integrated into practice and include: ‘coherence’ (understanding), ‘cognitive participation’ (what promotes engagement), ‘collective action’ (integration with existing systems), and ‘reflexive monitoring’ (evaluation).

Aim
To use NPT to investigate the integration of pharmacist IP in CP.

Method
NHS Scotland Pharmacy First Plus (PFP) is a community pharmacy IP service. Questionnaire items were developed using the NPT derived Normalisation MeAsure Development (NoMAD) tool for an online survey of all PFP IP pharmacists. Demographic data were analysed descriptively and scale scores (calculated from item scores for the 4 NPT constructs) were used for inferential analysis.

Results
There was a 73% (88/120) response rate. Greater than 90% ‘strongly agreed’/‘agreed’ to NoMAD items relating to most NPT constructs. However, responses to ‘collective action’ items were diverse with more participants answering ‘neither agree nor disagree’ or ‘disagree’. A statistically significant difference in NPT construct scale scores with significant p-values (ranging from p < 0.001 to p = 0.033) was shown on all the NPT constructs for the variable ‘On average, how often do you consult with patients under the PFP service?’.

Conclusion
This theory-based work offers perspectives on IP integration within CP. Despite its geographic focus this work offers insights relevant to wider contexts on IP integration. It shows ‘collective action’ focused ‘organisation’ and ‘group process’ challenges with a need for further work on staff training, resource availability and utilisation, working relationships, communication and management.
The tempest within: the origins and outcomes of intense national emotions in times of national division
Authors: Feinstein Yuval 
Published: May 2024

AbstractTheories of intense national emotions have focused on affection for the home nation and antagonism for national others but overlooked antagonism for fellow nationals. The article introduces a comprehensive theory of intense national emotions. It first discusses the sources of the potential energy stored in national identities, pointing to a combination of two factors: the nation is at once potent due to its capacity to shield against existential threats and precarious due to its dependence on the reproduction of contested narratives. The article then explains that events that—through a construction process elaborated in the text—seem to threaten or promise to alter perceived core elements of the nation (i.e., “nation-disrupting events”) evoke intense emotions. Next, the article explains why some periods of "hot" nationalism increase national division rather than unity. The conclusion proposes a promising direction for future research on intense national emotions as a mechanism of eventfulness.
Recruitment of Volunteers with Immigrant Backgrounds: The Impact of Structural and Individual Aspects
Authors: Sveen Silje , Anthun Kirsti Sarheim , Tingvold Laila 
Published: May 2024

AbstractVolunteering is associated with health-promoting benefits for both recipients and volunteers and may contribute to a more inclusive society. However, studies have shown a persistent pattern of social inequality among those who volunteer, and immigrants participate as volunteers less than the majority population. To date, approaches for recruiting immigrant populations have not been sufficiently examined, even though multicultural societies are becoming increasingly diverse. This study investigates how recruitment is carried out in voluntary organizations and how volunteers who are involved in recruitment reflect on the inclusion of citizens with immigrant backgrounds. Qualitative interviews were conducted with 18 volunteers and three employees with recruitment responsibility at five voluntary organizations engaged in welfare and community-related activities in a semirural district in Norway. Our findings show that different structural factors and individual aspects of the recruiter influence the recruitment of immigrants as volunteers. Large-scale organizations are more professionalized and more directed by fundings and frameworks and demand more qualifications due to their volunteer tasks. This might make inclusive recruitment more challenging. Small-scale organizations have more flexibility and less professionalized volunteer activities, making recruitment more inclusive. In addition, if the small-scale organizations are minority driven, it seems to positively influence the recruitment of immigrants through increased diversity sensitivity and more connections with immigrants through their social network.
Psychologically Traumatic Birth: Associations with Increased Drinking and Delayed Risk of Alcohol Harm in Mothers
Authors: Jones Andrew , Hunt Sally , Rose Abigail K. 
Published: May 2024

Abstract
Background
Experience of trauma is a risk factor for increased alcohol use. Childbirth can be psychologically traumatic but there is minimal research investigating whether psychological birth trauma (PBT) is a risk factor for increased maternal drinking or what factors are associated with alcohol use in mothers who have and who have not experienced PBT.

Method
An online observational survey of mothers with (n = 291) and without (n = 230) experience of PBT. Participants self-reported alcohol use and completed measures of alcohol harm, drinking motives, trauma (general and birth related) and mental health. Free text options were included to complement quantitative data.

Results
Irrespective of PBT status, stronger endorsement of negative reinforcement drinking motives (e.g. coping) predicted higher alcohol use and hazardous drinking. However, children’s age influenced this relationship. In mothers with a history of PBT, negative reinforcement drinking motives predicted weekly alcohol use and alcohol harm as their children grew older. In mothers without PBT, positive reinforcement drinking motives predicted greater weekly alcohol use as children grew older, but negative drinking motives predicted reduced drinking. Multiple aspects of the parental role influenced drinking (e.g. coping-based motives) and non-drinking (e.g. child well-being motives) behaviour. Changes in motives from pre-motherhood to motherhood focused around a shift from positive to negative reinforcement drinking motives. Mothers with PBT reported mental health symptoms as both a reason to drink and not to drink alcohol.

Conclusion
This study provides novel evidence on maternal alcohol use, and how PBT may influence drinking behaviour in mothers. Importantly, PBT may be a type of trauma which is associated with a delayed risk for maladaptive alcohol use and risk of alcohol harm. This evidence can facilitate more research aimed at understanding this important public health issue and can inform alcohol interventions tailored to the needs of mothers which consider the long-lasting impact of birth experience.
The role of semaphorin 3A on chondrogenic differentiation
Authors: Tsuboi Eri , Asakawa Yuki , Hirose Naoto , Yanoshita Makoto , Sumi Chikako , Takano Mami , Onishi Azusa , Nishiyama Sayuri , Kubo Naoki , Kita Daiki , Tanimoto Kotaro 
Published: May 2024

AbstractOsteoblast-derived semaphorin3A (Sema3A) has been reported to be involved in bone protection, and Sema3A knockout mice have been reported to exhibit chondrodysplasia. From these reports, Sema3A is considered to be involved in chondrogenic differentiation and skeletal formation, but there are many unclear points about its function and mechanism in chondrogenic differentiation. This study investigated the pharmacological effects of Sema3A in chondrogenic differentiation. The amount of Sema3A secreted into the culture supernatant was measured using an enzyme-linked immunosorbent assay. The expression of chondrogenic differentiation-related factors, such as Type II collagen (COL2A1), Aggrecan (ACAN), hyaluronan synthase 2 (HAS2), SRY-box transcription factor 9 (Sox9), Runt-related transcription factor 2 (Runx2), and Type X collagen (COL10A1) in ATDC5 cells treated with Sema3A (1,10 and 100 ng/mL) was examined using real-time reverse transcription polymerase chain reaction. Further, to assess the deposition of total glycosaminoglycans during chondrogenic differentiation, ATDC5 cells were stained with Alcian Blue. Moreover, the amount of hyaluronan in the culture supernatant was measured by enzyme-linked immunosorbent assay. The addition of Sema3A to cultured ATDC5 cells increased the expression of Sox9, Runx2, COL2A1, ACAN, HAS2, and COL10A1 during chondrogenic differentiation. Moreover, it enhanced total proteoglycan and hyaluronan synthesis. Further, Sema3A was upregulated in the early stages of chondrogenic differentiation, and its secretion decreased later. Sema3A increases extracellular matrix production and promotes chondrogenic differentiation. To the best of our knowledge, this is the first study to demonstrate the role of Sema3A on chondrogenic differentiation.
Pharmacotherapy in Stress Urinary Incontinence; A Literature Review
Authors: Tabei Seyed Sajjad , Baas Wesley , Mahdy Ayman 
Published: May 2024

Abstract
Purpose of Review
Stress urinary incontinence (SUI) is a commonly observed condition in females, as well as in males who have undergone prostatectomy. Despite the significant progress made in surgical techniques, pharmacotherapy has not yielded substantial outcomes within the clinical domain. This review aims to present a comprehensive overview of the existing pharmacotherapy options for stress urinary incontinence (SUI) and the emerging therapeutic targets in this field.

Recent Findings
One meta-analysis demonstrated that α-adrenergic medications are more efficacious in improving rather than curing SUI symptoms. One trial showed reduced pad weight gain with PSD-503, a locally administered α-adrenergic receptor agonist. New data show that duloxetine’s risk outweighs its benefits. One small-scale trial was found to support the use of locally administered estriol in improving subjective outcomes. Emerging targets include serotonin 5HT2C agonists, selective inhibitors of norepinephrine uptake, and myostatin inhibitors.

Summary
Only one of the evaluated drugs, duloxetine, has been approved by some countries. Currently, trials are evaluating novel targets. Systemic adverse effects such as gastrointestinal upset with duloxetine and orthostatic hypotension with α-adrenoceptor agonists have hampered the efficacy of drugs used to treat SUI in women and men.
Age-related patterns in mandibular third molar eruption: presenting new forensic age assessment reference data from a Chinese population
Authors: Timme Maximilian , Viktorov Jan , Steffens Laurin , Streeter Adam , Karch André , Guo Yu-Cheng , Schmeling Andreas 
Published: May 2024

AbstractValid reference data are essential for reliable forensic age assessment procedures in the living, a fact that extends to the trait of mandibular third molar eruption in dental panoramic radiographs (PAN). The objective of this study was to acquire valid reference data for a northern Chinese population. The study was guided by the criteria for reference studies in age assessment.To this end, a study population from China comprising 917 panoramic radiographs obtained from 430 females and 487 males aged between 15.00 and 25.99 years was analysed. Of the 917 PANs, a total of 1230 mandibular third molars were evaluated.The PANs, retrospectively evaluated, were performed for medical indication during the period from 2016 to 2021. The assessment of mandibular third molars was conducted using the staging scale presented by Olze et al. in 2012. Two independent examiners, trained in assessing PANs for forensic age estimation, evaluated the images. In instances where the two examiners diverged in their assessments these were subsequently deliberated, and a consensus stage was assigned.The mean age increased with higher stages for both teeth and both sexes. The minimum age recorded for stage D, indicating complete tooth eruption, was 15.6 years in females and 16.1 years in males. Consequently, the completion of mandibular third molar eruption was observed in both sexes well before reaching the age of 18. In light of our results, it is evident that relying solely on the assessment of mandibular third molar eruption may not be sufficient for accurately determining the age of majority. Contrary to previous literature, this finding of a completed eruption of the mandibular third molars in northern Chinese individuals is only suitable for detecting the completion of the 16th year of life in males according to our results. However, as the results are inconsistent compared to other studies in the literature, the trait should not be used as the only decisive marker to prove this age threshold in males from northern China.
Prioritising Important Plant Areas (IPAs) among the limestone karsts of Perak, Malaysia
Authors: Tan Joanne P. C. , Kiew Ruth , Darbyshire Iain 
Published: May 2024

SummaryThe flora of limestone karsts is exceptionally rich, due in particular to the wide array of microhabitats that limestone outcrops support. However, in Peninsular Malaysia limestone outcrops are heavily exploited for the quarry industry, cave temples, leisure venues and the development of geoparks, hence the limestone flora is amongst the most threatened biodiversity in the Peninsula. The 95 limestone karsts in Perak are currently known to support 538 species, representing c. 44.2% of the total limestone flora of Peninsular Malaysia. Of these, 13.2% (71 species) are endemic to the Peninsula of which 10 species are hyper-endemic (i.e. restricted to a single karst), 108 (20%) are threatened with extinction and 6 are considered likely to be Extinct. Typical of limestone in Peninsular Malaysia, a single karst harbours only a fraction of the total limestone flora such that a network of karsts is required to capture maximum plant diversity. Through evaluation of data from field surveys, herbarium specimens and the literature, 27 of the Perak karsts were found to trigger Important Plant Area (IPA) status, i.e. they harbour important populations of threatened species, outstanding botanical richness and/or are important examples of threatened habitats that in Perak include the foothills and limestone forest. Fifteen karsts were identified for priority conservation measures based on Conservation Priority Scores (CPS), a quantitative methodology based on endemic and threatened status. With the addition of two species-rich karst sites, 17 karsts are recommended as the first iteration of the network of priority IPA sites for Perak that together conserve 90% (483 species) of the Perak limestone plant diversity, 93% (66) of the endemic species and 94.4% (102) of the threatened species. CPS is found to be more reliable than using total species richness for assessing conservation importance and we also demonstrate that karst size and isolation are not primary indicators of range-restricted plant diversity. Significant knowledge gaps still exist, with 59 karsts in Perak without botanical surveys, and with many rare species having not been recollected for over 30 years, emphasising the need for further botanical research in these important and threatened landscapes.
Marsh-orchids of Canada: long-standing mysteries partially solved
Authors: Bateman Richard M. , Meades Susan J. , Denholm Ian , Tyteca Daniel , Hedrén Mikael 
Published: May 2024

SummaryBetween 1959 and 1988, three populations of purple-flowered terrestrial orchids attributable to Dactylorhiza subgenus Dactylorhiza were discovered in Canada. The populations at Timmins, Ontario, and St John's, Newfoundland were strongly marked on both flowers and leaves, in contrast with the anthocyanin-deficient population at Tilt Cove, Newfoundland. All three populations have since experienced a wide range of taxonomic assignments; debates are also ongoing regarding their origin and most appropriate conservation status. Here, we address these questions by combining detailed in situ morphometric analyses based on 52 characters with allozyme profiles and data from nrITS, 15 plastid microsatellites and seven nuclear microsatellites. The allozyme data alone are sufficient to both confirm allopolyploidy and categorically refute past assignments of these populations to D. incarnata, D. maculata, D. fuchsii, D. majalis or D. purpurella. Several morphometric characters, nuclear microsatellites and nrITS all reliably distinguish each of the three study populations, whereas the two sampled subpopulations from St John's proved near-identical morphologically. In contrast, morphological variation within each of the three populations is strikingly low, particularly in characters other than those influenced by plant vigour. Similarly, compared with 14 European populations, the three Canadian populations proved genetically impoverished (two were near-invariant) and likely experienced recent, extreme genetic bottlenecks during establishment. The three populations differ substantially, both morphologically and molecularly, therefore probably representing independent immigration events. Although clearly attributable to D. praetermissa, all three populations deviate significantly in morphology and DNA data from comparable populations sampled across Europe, preventing identification of their precise geographic origins. Any attempt to determine their mode or origin — through natural long-distance transport, or accidental or deliberate introduction by humans – is challenged to explain why three lineages of a single European Marsh-orchid species, each in different ways atypical of that species, arrived independently in North America whereas no other European dactylorchid species has become established there.
In Defense of Introspective Affordances
Authors: Gray David Miguel 
Published: May 2024

AbstractPsychological and philosophical studies have extended J. J. Gibson’s notion of affordances. Affordances are possibilities for bodily action presented to us by the objects of our perception. Recent work has argued that we should extend the actions afforded by perception to mental action. I argue that we can extend the notion of affordance itself. What I call ‘Introspective Affordances’ are possibilities for mental action presented to us by introspectively accessible states. While there are some prima facie worries concerning the non-perceptual nature of introspection, I will argue that our internal mental lives share enough commonalities with experiences in our environment to warrant this extension. I will demonstrate the value of introspective affordances by showing how they allow us to explain an underexplored aspect of thought insertion.
Pregnancy in Transmasculine, Non-binary, and Gender Expansive Patients
Authors: Cantwell Christopher J. 
Published: May 2024

Abstract
Purpose of Review
Transgender, non-binary, and gender expansive (TNGE) patients may choose to become pregnant and will require pregnancy-related care. To date, there has been limited research on pregnancy in this community. This review article aims to collate and summarize the most recent literature and make recommendations for providing pregnancy care to TNGE patients.

Recent Findings
TNGE patients can successfully undergo fertility preservation before or after starting their gender transition and may be able to continue gender-affirming hormone therapy with testosterone (GAHT-T) during the process. GAHT-T is potentially teratogenic and should be discontinued in pregnancy; however, GAHT-T does not appear to affect birth outcomes in TNGE patients. Clinicians should be aware that pregnancy can have profound physical and psychosocial impacts on TNGE patients, and patients frequently identify a lack of knowledgeable providers and welcoming care settings as barriers to care. Postpartum TNGE patients may be at increased risk of postpartum mood disorders. They may also choose to chest feed and require extra support and accommodations to be successful in this endeavor.

Summary
TNGE patients should be provided the same standard pregnancy-related care as their cisgender counterparts. Special consideration should be given to fertility preservation, contraception, postpartum mood disorders, and lactation/chest feeding as TNGE patients have unique needs within these areas. Much of the available research in this population is retrospective and qualitative with small sample sizes. Future research should focus on larger-scale quantitative studies to help develop evidenced-based, community-informed guidelines for care.
In-vacuum post-compression of optical probe pulses for relativistic plasma diagnostics
Authors: Lorenz S. , Grittani G. M. , Kondo K. , Kon A. , Liu Y.-K. , Sagisaka A. , Ogura K. , Nakanii N. , Huang K. , Bierwage A. , Namba S. , Ohiro H. , Pikuz T. A. , Koga J. K. , Chen P. , Kiriyama H. , Kando M. , Esirkepov T. Zh. , Bulanov S. V. , Pirozhkov A. S. 
Published: May 2024

Abstract
Ultrafast optical probing is a widely used method of underdense plasma diagnostic. In relativistic plasma, the motion blur limits spatial resolution in the direction of motion. For many high-power lasers the initial pulse duration of 30–50 fs results in a 10–15 μm motion blur, which can be reduced by probe pulse post-compression. Here we used the compression after compressor approach [Phys.-Usp. 62, 1096 (2019); JINST 17 P07035 (2022)], where spectral broadening is performed in thin optical plates and is followed by reflections from negative-dispersion mirrors. Our initially low-intensity probe beam was down-collimated for a more efficient spectral broadening and higher probe-to-self-emission intensity ratio. The setup is compact, fits in a vacuum chamber and can be implemented within a short experimental time slot. We proved that the compressed pulse retained the high quality necessary for plasma probing.