bluuebunny/crossref_metadata_2025_split huggingface dataset:
Negative regulation of wnt11 expression by Jnk signaling during zebrafish gastrulation
Authors: Seo Jungwon , Asaoka Yoichi , Nagai Yoko , Hirayama Jun , Yamasaki Tokiwa , Namae Misako , Ohata Shinya , Shimizu Nao , Negishi Takahiro , Kitagawa Daiju , Kondoh Hisato , Furutani‐Seiki Makoto , Penninger Josef M. , Katada Toshiaki , Nishina Hiroshi 
Published: April 2010

AbstractStress‐induced Sapk/Jnk signaling is involved in cell survival and apoptosis. Recent studies have increased our understanding of the physiological roles of Jnk signaling in embryonic development. However, still unclear is the precise function of Jnk signaling during gastrulation, a critical step in the establishment of the vertebrate body plan. Here we use morpholino‐mediated knockdown of the zebrafish orthologs of the Jnk activators Mkk4 and Mkk7 to examine the effect of Jnk signaling abrogation on early vertebrate embryogenesis. Depletion of zebrafish Mkk4b led to abnormal convergent extension (CE) during gastrulation, whereas Mkk7 morphants exhibited defective somitogenesis. Surprisingly, Mkk4b morphants displayed marked upregulation of wnt11, which is the triggering ligand of CE and stimulates Jnk activation via the non‐canonical Wnt pathway. Conversely, ectopic activation of Jnk signaling by overexpression of an active form of Mkk4b led to wnt11 downregulation. Mosaic lineage tracing studies revealed that Mkk4b‐Jnk signaling suppressed wnt11 expression in a non‐cell‐autonomous manner. These findings provide the first evidence that wnt11 itself is a downstream target of the Jnk cascade in the non‐canonical Wnt pathway. Our work demonstrates that Jnk activation is indispensable for multiple steps during vertebrate body plan formation. Furthermore, non‐canonical Wnt signaling may coordinate vertebrate CE movements by triggering Jnk activation that represses the expression of the CE‐triggering ligand wnt11. J. Cell. Biochem. 110: 1022–1037, 2010. © 2010 Wiley‐Liss, Inc.
Plumbagin inhibits proliferative and inflammatory responses of T cells independent of ROS generation but by modulating intracellular thiols
Authors: Checker Rahul , Sharma Deepak , Sandur Santosh K. , Subrahmanyam G. , Krishnan Sunil , Poduval T.B. , Sainis K.B. 
Published: April 2010

AbstractPlumbagin inhibited activation, proliferation, cytokine production, and graft‐versus‐host disease in lymphocytes and inhibited growth of tumor cells by suppressing nuclear factor‐κB (NF‐κB). Plumbagin was also shown to induce reactive oxygen species (ROS) generation in tumor cells via an unknown mechanism. Present report describes a novel role of cellular redox in modulation of immune responses in normal lymphocytes by plumbagin. Plumbagin depleted glutathione (GSH) levels that led to increase in ROS generation. The decrease in GSH levels was due to direct reaction of plumbagin with GSH as evinced by mass spectrometric and HPLC analysis. Further, addition of plumbagin to cells resulted in decrease in free thiol groups on proteins and increase in glutathionylation of proteins. The suppression of mitogen‐induced T‐cell proliferation and cytokine (IL‐2/IL‐4/IL‐6/IFN‐γ) production by plumbagin was abrogated by thiol antioxidants but not by non‐thiol antioxidants confirming that thiols but not ROS play an important role in biological activity of plumbagin. Plumbagin also abrogated mitogen‐induced phosphorylation of ERK, IKK, and degradation of IκB‐α. However, it did not affect phosphorylation of P38, JNK, and AKT. Our results for the first time show that antiproliferative effects of plumbagin are mediated by modulation of cellular redox. These results provide a rationale for application of thiol‐depleting agents as anti‐inflammatory drugs. J. Cell. Biochem. 110: 1082–1093, 2010. Published 2010 Wiley‐Liss, Inc.
Post‐transcriptional regulation of α‐smooth muscle actin determines the contractile phenotype of Dupuytren's nodular cells
Authors: Verjee Liaquat Suleman , Midwood Kim , Davidson Dominique , Eastwood Mark , Nanchahal Jagdeep 
Published: April 2010

AbstractThe objective was to study Dupuytren's myofibroblast cells in constrained collagen matrices in order to more closely emulate their in vivo environment and, to correlate their contractility with α‐smooth muscle actin (α‐SMA) expression and determine if dermal fibroblasts regulate Dupuytren's myofibroblast phenotype. Isotonic and isometric force contraction by cells isolated from Dupuytren's nodules, palmar and non‐palmar skin fibroblasts was measured in collagen matrices. The effect of co‐culturing nodule cells with dermal fibroblasts on isometric contraction was examined. Isometric contraction was correlated with levels of α‐SMA mRNA by pcr and protein by Western blotting, and α‐SMA distribution assessed by immunofluorescence. Dupuytren's nodule cells exhibited similar levels of isotonic contraction to both palmar and non‐palmar dermal fibroblasts. However, nodule cells generated high levels of isometric force (mean: 3.5 dynes/h), which continued to increase over 24 h to a maximum of 173 dynes. In contrast, dermal fibroblasts initially exhibited low levels of contraction (mean: 0.5 dynes/h) and reached tensional homeostasis on average after 15 h (range: 4–20 h), with a maximum force of 52 dynes. Although all three cell types had similar α‐SMA mRNA levels, increased levels of α‐SMA protein were observed in nodule cells compared to dermal fibroblasts. α‐SMA localised to stress fibres in 35% (range: 26–50%) of nodule cells compared to only 3% (range:0–6%) of dermal fibroblasts. Co‐cultures of Dupuytren's cells and dermal fibroblasts showed no contractile differences. The contractile phenotype of Dupuytren's myofibroblasts is determined by increased α‐SMA protein distributed in stress fibres, not by cellular mRNA levels. Dupuytren's cell contractility is not influenced by dermal fibroblasts. J. Cell. Physiol. 224: 681–690, 2010. © 2010 Wiley‐Liss, Inc.
Part 1 – Coherent Light: “From Chips to Ships”
Authors: Poprawe Reinhart 
Published: April 2010

AbstractThis article is the first part of a twopart series about the history as well as the future of laser technology. In the following therefore first the history processes and their perspectives shall be discussed as well as the effects on markets and megatrends, thus their societal impact shall be displayed. In part two of the series (published in Laser Technik Journal issue 3/2010) we will focus on the environmental “green” aspects of coherent light.
High‐Brightness Diode Lasers and New Wavelengths
Authors: Neukum Jörg , Biesenbach Jens , Köhler Bernd , Muy Nadine 
Published: April 2010

AbstractDesign considerations for highbrightness laser diodes in various new wavelength ranges are described, together with achieved results based on laser diode arrays, in the following referred to as laser bars. In addition, the new applications for laser diodes with such new wavelenghts are presented.
Singularities of o‐minimal Peano derivatives
Authors: Fischer Andreas 
Published: April 2010

AbstractThe notion of Peano differentiability generalizes the differentiability in the usual sense to higher order. Peano differentiable functions have derivatives which are sometimes differentiable or continuous or not even locally bounded. We give a complete characterisation of the sets in which Peano differentiable functions which are definable in an o‐minimal expansion of a real closed field are continuously differentiable. Thereby, we also distinguish between several kinds of discontinuities (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Integral combinations of Heavisides
Authors: Kainen Paul C. , Kůrková Věra , Vogt Andrew 
Published: April 2010

AbstractA sufficiently smooth function of d variables that decays fast enough at infinity can be represented pointwise by an integral combination of Heaviside plane waves (i.e., characteristic functions of closed half‐spaces). The weight function in such a representation depends on the derivatives of the represented function. The represen‐tation is proved here by elementary techniques with separate arguments for even and odd d, and unifies and extends various results in the literature (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Women urinate in the standing position do not increase post‐void residual urine volumes
Authors: Chou Eric Chieh‐Lung , Chang Chao‐Hsiang , Chen Chi‐Cheng , Wu Hsi‐Chin , Wu Po‐Lun , Chen Kuo‐Liang 
Published: April 2010

AbstractAimsThe effects of standing while voiding have seldom been investigated in women. We evaluate urodynamic parameters of voiding while standing in healthy women using uroflowmetry and post‐void residual urine volume assessment. Results are compared with crouching and sitting.MethodsBetween July and October, 2008, a total of 30 healthy, nulliparous female volunteers were enrolled. Ages were 22–37 (mean: 28±4). Urodynamic studies were performed for all in sitting, crouching and standing positions; 3, 3 and 5 times in each position, respectively. Volunteers used homemade auxiliary appliances for collecting urine from the urethra and draining it forward when standing. Volume, maximum flow rate, mean flow rate and post‐void residual urine volume were compared.ResultsMaximum and average flow rates in the sitting and standing positions were significantly different, but not between sitting and crouching or between crouching and standing. There were no differences in voided volume and post‐void residual urine volume. There's no apparent learning curve for women in the standing position.ConclusionsThough flow rates are decreased while standing, post‐void residual volume is not significantly different. Women have another choice for voiding in public restrooms. Neurourol. Urodynam. 29:1299–1300, 2010. © 2010 Wiley‐Liss, Inc.
Serum prostate‐specific antigen levels reflect the androgen milieu in patients with localized prostate cancer receiving androgen deprivation therapy: Tumor malignant potential and androgen milieu
Authors: Takizawa Itsuhiro , Nishiyama Tsutomu , Hara Noboru , Isahaya Etsuko , Hoshii Tatsuhiko , Takahashi Kota 
Published: April 2010

AbstractBACKGROUNDAlthough androgen deprivation therapy (ADT) has a marked impact on the androgen milieu in vivo and outcomes of prostate cancer (PCa), it remains unclear which parameters reflect the androgen milieu during ADT or whether the milieu is associated with PCa aggressiveness.METHODSSeventy‐two patients with localized PCa were prospectively studied based on their blood samples before and after ADT for 6 months. Serum androgens and related values were measured.RESULTSBefore ADT, there was no correlation between the serum prostate‐specific antigen (PSA) and androgen levels. After ADT, the serum PSA levels were correlated with each level of serum testosterone, dihydrotestosterone, androstenedione, dehydroepiandrosterone‐sulfate (DHEA‐S), and 3alpha‐diol G (P < 0.010 in all). Before ADT, patients with Gleason score of ≥8 were likely to have lower serum testosterone levels than those with Gleason score of ≤6 (P = 0.058). After ADT, conversely, the testosterone levels in patients with Gleason score of ≥8 appeared to be higher than in those with Gleason score of ≤6 (P = 0.060). The serum DHEA‐S level was correlated with Gleason score before and after ADT (P = 0.050 and P = 0.040, respectively).CONCLUSIONSThe serum PSA levels well reflect the androgen milieu in localized PCa patients receiving ADT, which can be explained by the Saturation Model and disease control. The androgen milieu in men with high Gleason score PCa is probably less affected by conventional ADT than that in men with low score cancer, which was suggested to be associated with adrenal androgen levels. Prostate 70: 1395–1401, 2010. © 2010 Wiley‐Liss, Inc.
The catch bond mechanism between von Willebrand factor and platelet surface receptors investigated by molecular dynamics simulations
Authors: Interlandi Gianluca , Thomas Wendy 
Published: April 2010

AbstractThe multi‐domain protein von Willebrand factor is crucial in the blood coagulation process at high shear. The A1 domain binds to the platelet surface receptor glycoprotein Ibα (GpIbα) and this interaction is known to be strengthened by tensile force. The molecular mechanism behind this observation was investigated here by molecular dynamics simulations. The results suggest that the proteins unbind through two distinct pathways depending whether a high‐tensile force is applied or whether unbinding happens through thermal fluctuations. In the high‐force unbinding pathway the A1 domain was observed to rotate away from the C‐terminus of GpIbα. In contrast, during thermal unbinding the A1 domain rotated in the opposite direction as in the high‐force pathway and the distance between the terminii of A1 and the GpIbα C‐terminus shortened. This shortening was reduced and the lifetime of the bond extended if a moderate tensile force was applied across the complex. This suggests that the thermal unbinding pathway is inhibited by a moderate tensile force which is in agreement with the catch bond property shown previously in single molecule experiments. A designed mutant of GpIbα is suggested here in order to test in vitro the thermal unbinding pathway observed in silico. Proteins 2010. © 2010 Wiley‐Liss, Inc.
Motorization of a surgical microscope for intra‐operative navigation and intuitive control
Authors: Finke M. , Schweikard A. 
Published: April 2010

AbstractBackgroundDuring surgical procedures, various medical systems, e.g. microscope or C‐arm, are used. Their precise and repeatable manual positioning can be very cumbersome and interrupts the surgeon's work flow. Robotized systems can assist the surgeon but they require suitable kinematics and control. However, positioning must be fast, flexible and intuitive.MethodsWe describe a fully motorized surgical microscope. Hardware components as well as implemented applications are specified. The kinematic equations are described and a novel control concept is proposed.ResultsOur microscope combines fast manual handling with accurate, automatic positioning. Intuitive control is provided by a small remote control mounted to one of the surgical instruments. Positioning accuracy and repeatability are < 1 mm and vibrations caused by automatic movements fade away in about 1 s.ConclusionThe robotic system assists the surgeon, so that he can position the microscope precisely and repeatedly without interrupting the clinical workflow. The combination of manual und automatic control guarantees fast and flexible positioning during surgical procedures. Copyright © 2010 John Wiley & Sons, Ltd.
Properties and morphology of poly(vinyl chloride) blends with solid‐state‐chlorinated polyethylene
Authors: Zhang Yuhong , He Peixin 
Published: April 2010

AbstractCorrelations of the stress‐strain behavior and impact strength of poly(vinyl chloride) (PVC) blends with different amounts and chlorine contents of solid‐state‐chlorinated polyethylene (CPE) were studied. The relationships between the morphology and properties of the PVC/CPE blends also were investigated. The results of dynamic mechanical analysis and transmission electron microscopy showed that PVC/CPE blends are partially compatible systems and that a certain interaction exists between the two phases. When the amount of CPE (chlorine content, 36–42%) was 7–15 phr (parts by weight per hundred parts of resin), an essentially perfect CPE network was formed, and the blends showed better impact resistance. A Brabender Plasticorder study revealed that CPE can promote the plasticity of PVC and improve its processability. J. VINYL ADDIT. TECHNOL., 2010. © 2010 Society of Plastics Engineers
3D analyses of cavitation instabilities accounting for plastic anisotropy
Authors: Legarth B.N. , Tvergaard V. 
Published: April 2010

AbstractFull three dimensional cell model analyses are carried out for a solid containing a single small void, in order to determine the critical stress levels for the occurrence of cavitation instabilities. The material models applied are elastic‐viscoplastic, with a small rate‐hardening exponent, and the main focus is on the effect of different degrees of plastic anisotropy. Loading cases are considered, where all the macroscopic principal stresses differ. The numerical quasi‐static solutions are obtained by a full transient analysis of the equations of motion, in which the loading is applied so slowly that the quasi‐static solution is well approximated. A special procedure is used to strongly reduce the loading rate a little before the instability occurs. It is found that plastic anisotropy has a significant effect on the level of the critical stress for cavitation instabilities.
Hémangiome caverneux du cordon spermatique : à propos d’un cas et revue de la littérature
Authors: Elbaghouli M. , Aboutaib R. , Dakir M. , Rabii R. , Meziane F. 
Published: April 2010

Résumé
L’hémangiome caverneux du cordon spermatique est une tumeur vasculaire bénigne très rare. Seuls quelques cas ont été rapportés dans la littérature. Le diagnostic clinique est plutôt difficile, et il est, en général, de découverte anatomopathologique. Ses principales particularités sont la gravité de ses complications et la possibilité d’involution spontanée. Nous rapportons le cas d’un patient de 26 ans, traité pour tuberculose ganglionnaire il y a deux ans, et, chez qui, l’échographie a orienté vers ce diagnostic. Le traitement consistait en une tumorectomie. La discussion concerne la définition, l’étiopathogénie, les aspects histologiques, cliniques, les moyens diagnostiques, les complications et le traitement.
Prevalence, characteristics and comorbidities of anxiety disorders in France: Results from the “Mental Health in General Population” Survey (MHGP)
Authors: Leray E. , Camara A. , Drapier D. , Riou F. , Bougeant N. , Pelissolo A. , Lloyd K.R. , Bellamy V. , Roelandt J.L. , Millet B. 
Published: April 2010

AbstractPurposeOnly a few European countries have carried out large, community-based, national surveys about psychiatric morbidity. Here is presented the first national French survey, aiming to estimate the prevalence of anxiety disorders and associated comorbidities according to sociodemographic characteristics.Materials and methodsThe Mental Health in General Population (MHGP) database is derived from a representative national survey of the French adult population (n= 36,105), conducted between 1999 and 2003. Data collection was done using an anonymous face-to-face interview. The presence of anxiety disorders (generalized anxiety disorder, panic disorder, agoraphobia, social phobia and post-traumatic stress disorder) was assessed using the Mini International Neuropsychiatric Interview.ResultsThe overall prevalence of anxiety disorders was estimated to be 21.6%, generalized anxiety disorder being the most prevalent one (12.8%). Women, young people, and people earning low income were identified as the more at risk. Major depressive episode, alcohol abuse and drug addiction frequently co-occur with anxiety disorders (28.3, 4.4 and 2.8% respectively).ConclusionsThe MHGP study showed that anxiety disorders are highly prevalent in France with a high frequency of comorbidities. Our results highlight the need for considering anxiety disorders as a public health priority in France as well as in other European countries.
Journey to Confidence: Women's Experiences of Pain in Labour and Relational Continuity of Care
Authors: Leap Nicky , Sandall Jane , Buckland Sara , Huber Ulli 
Published: April 2010

Introduction: An evaluation carried out at King's College Hospital Foundation National Health Service Trust in London identified that women who received continuity of carer from the Albany Midwifery Practice were significantly less likely to use pharmacological pain relief when comparisons were made with eight other midwifery group practices and the local maternity service as a whole. This study was designed to explore women's views of this phenomenon.Methods: We conducted a thematic analysis of semistructured, audiotaped, in‐depth interviews with 10 women who reflected on their experiences of preparation and support for pain in labour and midwifery continuity of carer with Albany midwives, using a qualitative descriptive methodological approach.Results: Women reflected positively on how, throughout pregnancy and labour, their midwives promoted a sense of their ability to cope with the challenge of labour pain. This building of confidence was enabled through a relationship of trust that developed with their midwives and the value of hearing other women's stories during antenatal groups. These experiences enhanced women's ability to overcome fears and self‐doubt about coping with pain and led to feelings of pride, elation, and empowerment after birth.Discussion: Women valued being encouraged and supported to labour without using pharmacological pain relief by midwives with whom they developed a trusting relationship throughout pregnancy. Features of midwifery approaches to pain in labour and relational continuity of care have important implications for promoting normal birth and a positive experience of pregnancy, labour, and birth for women.J Midwifery Womens Health 2010;55:234–242 c&#x030a; 2010 by the American College of Nurse‐Midwives.
Middle Paleozoic and Early Mesozoic anorogenic magmatism of the South Yenisei Ridge: first geochemical and geochronological data
Authors: Vernikovskaya A.E. , Vernikovsky V.A. , Matushkin N.Yu. , Romanova I.V. , Berejnaya N.G. , Larionov A.N. , Travin A.V. 
Published: April 2010

Abstract
In this paper we present complex geological, petrographic and geochronological data of the study of intermediate and acid composition intrusive and volcanogenic rocks from the Porozhnaya massif of the South Yenisei Ridge. For the first time in the Yenisei Ridge Devonian and Triassic U-Pb age values (SHRIMP method) have been obtained for leucogranites—387 ± 5 Ma and alkaline trachytes—240 ± 3 Ma, which allows us to attribute them to two different complexes, despite the fact that these rocks were formed within the same Severnaya riftogenic structure. Geochronological Ar-Ar data (392–387 Ma) for micas from paragneisses and leucogranitic dikes of the Yenisei suture zone on whose extension the Severnaya riftogenic structure is located are also given in this study. These data on Devonian tectonic-magmatic events in the South Yenisei Ridge agree well with coeval events of continental rifting—the formation of intrusive and volcanogenic rocks of the Agul graben in the Prisayan region and the Minusa basin in the Altai–Sayan folded area. The forming of alkaline trachytes and alkaline syenites of the Severnaya riftogenic structure, for which an age of 240 ± 3 Ma has been established, is related to the trap magmatism of the Siberian platform.