bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interaction of endocannabinoid system and steroid Hormones in the control of colon cancer cell growth
Authors: Proto Maria C. , Gazzerro Patrizia , Di Croce Luciano , Santoro Antonietta , Malfitano Anna M. , Pisanti Simona , Laezza Chiara , Bifulco Maurizio 
Published: March 2011

AbstractIncreasing evidence suggest the role of the cannabinoid receptors (CBs) in the control of cell survival or death and signaling pathways involved in tumor progression. Cancer cell lines are characterized by a subtle modulation of CB levels which produces a modified responsiveness to specific ligands, but the molecular mechanisms underlying these events are poorly and partially understood. We previously provided evidence that the endocannabinoid (EC) anandamide (AEA) exerts anti‐proliferative effect likely by modulation of the expression of genes involved in the cellular fate. In this study we focused on the role of the CB1 receptor, ECs, and steroids in the mechanisms involved in colorectal cancer (CRC) cell growth inhibition in vitro. We demonstrated that, in DLD1 and SW620 cells, 17β‐estradiol induced a specific and strong up‐regulation of the CB1 receptor by triggering activation of the CB1 promoting region, localized at the exon 1 of the CNR1 gene. Moreover, treatment of DLD1 and SW620 cells with Met‐F‐AEA, a stable AEA‐analogous, or URB597, a selective inhibitor of FAAH, induced up‐regulation of CB1 expression by co‐localization of PPARγ and RXRα at the promoting region. Finally, increased availability of AEA, of both exogenous and endogenous sources, induced the expression of estrogen receptor‐beta in both cell lines. Our results partially elucidated the role of EC system in the molecular mechanisms enrolled by steroids in the inhibition of colon cancer cell growth and strongly suggested that targeting the EC system could represent a promising tool to improve the efficacy of CRC treatments. J. Cell. Physiol. 227: 250–258, 2012. © 2011 Wiley Periodicals, Inc.
MicroRNAs: Allies or Foes in erythropoiesis?
Authors: Byon John C.H. , Papayannopoulou Thalia 
Published: March 2011

AbstractMicroRNAs are small non‐coding RNAs that negatively regulate gene expression through mRNA degradation or translational repression. It is becoming increasingly recognized that miRNAs play central roles in almost all cellular processes, and especially during development. The function of miRNAs in hematopoiesis, including erythropoiesis, is beginning to be elucidated. In this review, we will focus on what is known about miRNA function in various aspects of erythropoiesis and red cell physiology. J. Cell. Physiol. 227: 7–13, 2012. © 2011 Wiley Periodicals, Inc.
Gremlin1 is required for skeletal development and postnatal skeletal homeostasis
Authors: Canalis Ernesto , Parker Kristen , Zanotti Stefano 
Published: March 2011

AbstractGremlin is an antagonist of bone morphogenetic proteins, and its overexpression causes suppressed osteoblastogenesis and osteopenia. Inactivation of Grem1 results in severe developmental defects, but the consequences of the global inactivation of Grem1 on the postnatal skeleton are not known. To study the function of gremlin, Grem1 was inactivated by homologous recombination, and mice were maintained in a C57BL/6/FVB mixed genetic background due to embryonic and neonatal lethality in the uniform C57BL/6 background. Grem1 null mice exhibited developmental skeletal abnormalities, leading to incomplete formation of metatarsal bones and of fore limbs and hind limbs. Grem1 null mice exhibited decreased weight and body fat and shortened femoral length. Bone histomorphometric and microarchitectural analyses of distal femurs revealed decreased bone volume and increased bone formation in 1‐month‐old Grem1 null mice. Trabecular femoral bone volume was restored in older Grem1 null female mice, and to a lesser extent in male mice. Vertebral microarchitecture confirmed the osteopenia observed in 1‐month‐old Grem1 null mice and demonstrated recovery of trabecular bone in older female, but not in older male Grem1 null mice, which exhibited persistent vertebral osteopenia. In conclusion, Grem1 is not only necessary for skeletal development, but also for postnatal skeletal homeostasis; its inactivation causes osteopenia, which is partially reversed in a spatial, temporal, and sex‐dependent manner due to an increase in bone formation. J. Cell. Physiol. 227: 269–277, 2012. © 2011 Wiley Periodicals, Inc.
The science of stem cell biobanking: Investing in the future
Authors: Diaferia Giuseppe R. , Cardano Marina , Cattaneo Monica , Spinelli Chiara C. , Dessì Sara S. , DeBlasio Pasquale , Biunno Ida 
Published: March 2011

AbstractThe use of human stem cells in biomedical research projects is increasing steadily and the number of cells that are being derived develops at a remarkable pace. However, stem cells around the world are vastly different in their provenance, programming, and potentials. Furthermore, knowledge on the actual number of cell types, their derivation, availability, and characteristics is rather sparse. Usually, “colleague‐supply” avenues constantly furnish cells to laboratories around the world without ensuring their correct identity, characterization, and quality. These parameters are critical if the cells will be eventually used in toxicology studies and drug discovery. Here, we outline some basic principles in establishing a stem cell‐specific bank. J. Cell. Physiol. 227: 14–19, 2012. © 2011 Wiley Periodicals, Inc.
Genetic variation of the hemagglutinin of avian influenza virus H9N2
Authors: Song Xiao‐feng , Han Ping , Chen Yi‐Ping Phoebe 
Published: March 2011

AbstractAvian influenza virus H9N2 has become the dominant subtype of influenza which is endemic in poultry. The hemagglutinin, one of eight protein‐coding genes, plays an important role during the early stage of infection. The adaptive evolution and the positively selected sites of the HA (the glycoprotein molecule) of H9N2 subtype viruses were investigated. Investigating 68 hemagglutinin H9N2 avian influenza virus isolates in China and phylogenetic analysis, it was necessary that these isolates were distributed geographically from 1994, and were all derived from the Eurasian lineage. H9N2 avian influenza virus isolates from domestic poultry in China were distinct phylogenetically from those isolated in Hong Kong, including viruses which had infected humans. Seven amino acid substitutions (2T, 3T, 14T, 165D, 197A, 233Q, 380R) were identified in the HA possibly due to positive selection pressure. Apart from the 380R site, the other positively selected sites detected were all located near the receptor‐binding site of the HA1 strain. Based on epidemiological and phylogenetics analysis, the H9N2 epidemic in China was divided into three groups: the 1994–1997 group, the 1998–1999 group, and the 2000–2007 group. By investigating these three groups using the maximum likelihood estimation method, there were more positive selective sites in the 1994–1997 and 1998–1999 epidemic group than the 2000–2007 groups. This indicates that those detected selected sites are changed during different epidemic periods and the evolution of H9N2 is currently slow. The antigenic determinant or other key functional amino acid sites should be of concern because their adjacent sites have been under positive selection pressure. The results provide further evidence that the pathogenic changes in the H9N2 subtype are due mainly to re‐assortment with other highly pathogenic avian influenza viruses. J. Med. Virol. 83:838–846, 2011. © 2011 Wiley‐Liss, Inc.
Chitosan as an adjuvant for poliovaccine
Authors: Ghendon Y. , Markushin S. , Akopova I. , Koptiaeva I. , Krivtsov G. 
Published: March 2011

AbstractThe use of inactivated poliomyelitis vaccine is very important for eradicating poliomyelitis. However, this vaccine is not available readily in underdeveloped countries due to the high cost. Adjuvants can improve the immunogenicity of a vaccine and reduce the antigen dose required for vaccination, thus lowering the cost of the vaccine. Chitosan glutamate solution and a chitosan sulfate micro/nanoparticle suspension were tested as adjuvants for Imovax‐inactivated poliovaccine and for inactivated monovalent poliovirus type 1, 2, and 3 vaccines obtained by inactivation of the attenuated Sabin poliovirus strains. Inactivated vaccines admixed with either chitosan glutamate or chitosan sulfate micro/nanoparticles and administered to mice showed significantly enhanced immunogenicity to poliovirus type 1, 2, and 3 strains compared to the respective vaccines administered without chitosan. Chitosan preparations increased the immunogenicity of 1:2 and 1:4 diluted inactivated Sabin strain preparations in mice 8‐ to 16‐fold, so that the neutralizing antibody titers after vaccination with adjuvanted diluted vaccine were equal to those obtained after vaccination with undiluted vaccine administered without chitosan. Neutralizing antibodies could be detected in sera of rats vaccinated with undiluted, 1:10, and 1:100 diluted Imovax vaccine admixed with chitosan sulfate micro/nanoparticles, although in the control group, vaccination only with the undiluted vaccine resulted in antibody production. These results show that the chitosan glutamate solution and chitosan sulfate micro/nanoparticle suspension can significantly improve the immunogenicity of various poliovaccines, and reduce the effective antigen dose. J. Med. Virol. 83:847–852, 2011. © 2011 Wiley‐Liss, Inc.
Characterization of human immunodeficiency virus (HIV1C) variants in peripheral blood mononuclear cells and spermatozoa
Authors: Jadhav Shivaji K. , Velhal Shilpa M. , Deshpande Alaka , Maitra Anurupa , Chinnaraj Saravanan , Bandivdekar Atmaram H. 
Published: March 2011

AbstractThe presence of distinct viral variants in different cells and secretions of the same person influences the transmission of HIV as well as the response to the host defense and to therapy. Sperm‐associated virus is also a risk factor for sexual transmission of HIV. Characterization of the C2–V3 region of HIV1C env gene by the Heteroduplex Mobility Assay (HMA) and sequencing demonstrated the presence of distinct variants in the peripheral blood mononuclear cells (PBMCs) and the sperm of the same individual (n = 6). The translated amino acid sequences of HIV variants in the PBMCs of all the study participants (n = 12) and spermatozoa of the six participants characterized showed the presence of distinct variants with different numbers of N‐linked glycosylation (NLG) sites. Infectivity of PBMCs of these persons by co‐culture with PBMCs from healthy individuals as detected by the p24 levels in the culture supernatant did not show a correlation with the blood plasma viral load. Interestingly, the infectivity of the sperm samples from four of the five individuals showed positive correlation with the viral load in seminal plasma. The study suggests the presence of distinct viral variants in the sperm and PBMCs of the same person with differential infectivity, and the NLG sites may be associated with the affinity of HIV to receptor/co‐receptor usages as well as affinity toward neutralizing antibodies which may influence the risk of sperm associated virus in sexual transmission of HIV and transmit the virus further to distal cells. J. Med. Virol. 83:760–767, 2011. © 2011 Wiley‐Liss, Inc.
Efficacy of interferon‐based antiviral therapy in patients with chronic hepatitis C infected with genotype 5: A meta‐analysis of two large prospective clinical trials
Authors: D'Heygere François , George Christophe , Van Vlierberghe Hans , Decaestecker Jochen , Nakad Antoine , Adler Michael , Delwaide Jean , Laureys Annick , Nevens Frederik 
Published: March 2011

AbstractThe characteristics and response rate to pegylated interferon and ribavirin (PEG‐INF + RBV) of patients with chronic hepatitis C infected with genotype 5 are poorly documented. A meta‐analysis of two large phase III/IV prospective randomized clinical trials conducted in Belgium in patients with chronic hepatitis C (n = 1,073 patients) was performed in order to compare the response to antiviral therapy of hepatitis C virus (HCV) genotype 5 with that of other HCV genotypes. A subset of HCV‐1 infected patients selected from within the study database were selected to match the HCV‐5 sample for known prognostic factors. In Belgium HCV‐5 is responsible for a significant minority of cases of chronic hepatitis C CHC (4.5%) and is characterized by a more advanced age (58.4 years), a high frequency of cirrhosis (27.7%), a specific mode of HCV acquisition, and a particular geographic origin (66.7% of patients from West Flanders). The primary comparative analysis showed that response to treatment with PEG‐INF + RBV of HCV‐5 is similar to HCV‐1 and lower compared to HCV‐2/3. The analysis of the matched patient subgroup demonstrates that the HCV‐5 “intrinsic sensitivity” to PEG‐IFN + RBV therapy is identical to HCV‐1, with a sustained virological response of 55% in both groups. In contrast to previous publications, this meta‐analysis suggests that HCV‐5 response to treatment is closer to HCV‐1 than to HCV‐2/3 and suggests that in Belgium HCV‐5 infection should be treated with the same antiviral regimen as HCV‐1. J. Med. Virol. 83:815–819, 2011. © 2011 Wiley‐Liss, Inc.
Hepatitis B virus and sexual behavior in Rakai, Uganda
Authors: Stabinski Lara , Reynolds Steven J. , Ocama Ponsiano , Laeyendecker Oliver , Serwadda David , Gray Ron H. , Wawer Maria , Thomas David L. , Quinn Thomas C. , Kirk Gregory D. 
Published: March 2011

AbstractHIV and hepatitis B virus (HBV) co‐infection poses important public health considerations in resource‐limited settings. Demographic data and sera from adult participants of the Rakai Health Sciences Program Cohort in Southwestern Uganda were examined to determine HBV seroprevalence patterns in this area of high HIV endemicity prior to the introduction of anti‐retroviral therapy. Commercially available EIAs were used to detect prevalent HBV infection (positive for HBV core antibody [anti‐HBc] and/or positive HBV surface antigen [HBsAg]), and chronic infection (positive for HBsAg). Of 438 participants, 181 (41%) had prevalent HBV infection while 21 (5%) were infected chronically. Fourteen percent of participants were infected with HIV. Fifty three percent showed evidence of prevalent HBV infection compared to 40% among participants infected with HIV (P = 0.067). Seven percent of participants infected with HIV were HBsAg positive compared to 4% among participants not infected with HIV (P = 0.403). The prevalence of prevalent HBV infection was 55% in adults aged >50 years old, and 11% in persons under 20 years. In multivariable analysis, older age, HIV status, and serologic syphilis were significantly associated with prevalent HBV infection. Transfusion status and receipt of injections were not significantly associated with HBV infection. Contrary to expectations that HBV exposure in Uganda occurred chiefly during childhood, prevalent HBV infection was found to increase with age and was associated sexually transmitted diseases (HIV and syphilis.) Therefore vaccination against HBV, particularly susceptible adults with HIV or at risk of HIV/STDs should be a priority. J. Med. Virol. 83:796–800, 2011. © 2011 Wiley‐Liss, Inc.
Viral etiology of aseptic meningitis among children in Southern Iran
Authors: Hosseininasab Ali , Alborzi Abdolvahab , Ziyaeyan Mazyar , Jamalidoust Marzieh , Moeini Mahsa , Pouladfar Gholamreza , Abbasian Amin , Kadivar Mohamad Rahim 
Published: March 2011

AbstractAseptic meningitis refers to a clinical syndrome of meningeal inflammation in which bacteria cannot be identified in the cerebrospinal fluid (CSF). The viral etiology and the epidemiological, clinical, and laboratory characteristics of aseptic meningitis among children aged 2 months to 15 years in Shiraz, southern Iran were determined. From May 2007 to April 2008, 65 patients were admitted to the hospital with aseptic meningitis. Seven viruses, non‐polio human enteroviruses, mumps virus, herpes simplex virus (HSV), varicella‐zoster virus (VZV), human cytomegalovirus (HCMV), human herpes virus type 6 (HHV‐6), and Epstein–Barr virus (EBV) were investigated by polymerase chain reaction (PCR) method. Viruses were detected in 30 (46.2%) patients in whom non‐polio human enterovirus and mumps virus were detected in 13 (43.3%) and 11 (36.7%), respectively. The remaining 6 (20%) of the cases were caused by HSV, VZV, HCMV, and HHV‐6. Haemophilus influenzae and non‐polio human enterovirus were detected in one patient simultaneously. Viral meningitis was found to be more frequent during spring and summer. The majority (66.6%) of the patients were treated in the hospital for 10 days and had received antibiotics in the case of bacterial meningitis. Rapid diagnosis of viral meningitis using PCR testing of CSF can help shorten hospitalization, and avoid the unnecessary use of antibiotics. J. Med. Virol. 83:884–888, 2011. © 2011 Wiley‐Liss, Inc.
Kinetics of aquation of Co(III) complex in binary aqueous mixtures and effect of multiparameters of the solvent on the reaction rate
Authors: El‐Subruiti Gehan M. , Fathalla Magda F. , El‐Marassi Yasser R. 
Published: March 2011

AbstractKinetics and solvent effects of the aquation of trans[Co(4‐(Etpy)4Cl2]+ have been studied in ethanol + water ranging from 0 to 60% (v/v) and urea + water of various solvent compositions up to 40% (w/w) of organic solvent. Thermodynamic activation parameters were computed and discussed in terms of the solvation effect. Isokinetic temperature within the experimental range revealed that the existence of the compensation effect arising from the solute–solvent interaction. Nonlinear plots of log k with D−1 suggest that changes in the solvent structure are an important factor that influences these rates. The influence of the added cosolvent on reactivity was analyzed in light of various simple and multiple regression equations using Kirkwood, ET(30), and Kamlet–Taft parameters. The obtained results showed that the solvation phenomenon plays a dominant role in the aquation. © 2011 Wiley Peiodicals, Inc. Int J Chem Kinet 43: 230–237, 2011
Biological geotextiles as a tool for soil moisture conservation
Authors: Kertész Á. , Szalai Z. , Jakab G. , Tóth A. , Szabó Sz. , Madarász B. , Jankauskas B. , Guerra A. , Bezerra J. F. R. , Panomtaranichagul M. , Thu D. Chau , YI Z. 
Published: March 2011

AbstractGeotextiles have an important influence on soil moisture conditions. It is well known that the application of geotextiles increases soil moisture content of the soil, but there is a lack of information on how the different mats keep the soil moisture. The objective of this paper is to present the effect of biological geotextiles on soil moisture dynamics of the topsoil and to compare the effectiveness of various geotextiles in conserving soil moisture as well as comparing their role in soil moisture dynamics in the different climatic zones. Soil moisture measurements were carried out in the framework of the BORASSUS project at six study sites in Brazil, China, Hungary, Lithuania, Thailand and Vietnam. Soil moisture was measured by gravimetric method. All together six different kinds of biological geotextiles (Borassus, Buriti, Bamboo, Jute, Maize and Rice) and one synthetic geotextile were used. To study soil moisture dynamics of the geotextiles applied in Hungary 1000 cm3soil monoliths were covered by Jute, Buriti and Borassus mats. The effects of natural rainfall events on soils and geotextiles were examined in detail. According to the results of this paper there is not much difference concerning the effect of biological geotextiles made from various materials on soil moisture conservation. There are, however, remarkable differences in conserving soil moisture according to the geographical location of the study sites. The favourable effect of geotextiles on soil moisture conservation could be statistically justified if annual precipitation amount is over 700 mm and the annual temperature range is below 28°C. Copyright © 2011 John Wiley & Sons, Ltd.
ASSESSING SPATIAL‐TEMPORAL EVOLUTION PROCESSES OF KARST ROCKY DESERTIFICATION LAND: INDICATIONS FOR RESTORATION STRATEGIES
Authors: Bai X.‐Y. , Wang S.‐J. , Xiong K.‐N. 
Published: March 2011

ABSTRACTKarst rocky desertification (KRD) has become one of the most important eco‐environmental problems in China. In order to put forward valid restoration strategies, the spatial‐temporal KRD evolution processes were analyzed mathematically using 1:100 000 scale digital KRD distribution maps of Guizhou Province obtained from interpreting Landsat images from 1986, 1995, and 2000. The results showed that: (1) no obvious change took place in the total area of KRD land, but the mutual transformation of different types of KRD land was remarkable. (2) The change patterns of KRD land were classified into three types: simple mode, continuous mode, and reverted mode. (3) The total change rate of KRD land was 398·31 km2 per year. (4) The higher the rank of KRD land is, the lower the change rate of KRD land will be. (5) Moderate KRD land had the fastest change frequency and was feasible to change to another type. On the whole, these indicated that the expansion of KRD had been brought under control because of the successful execution many ecological construction programmes. However, new KRD land appeared meanwhile owing to ‘backward’ eco‐social development, and this should be clearly recognized. Copyright © 2011 John Wiley & Sons, Ltd.
Tibialis anterior architecture, strength, and gait in individuals with cerebral palsy
Authors: Bland Daniel C. , Prosser Laura A. , Bellini Lindsey A. , Alter Katharine E. , Damiano Diane L. 
Published: March 2011

AbstractIntroduction: The relationship of tibialis anterior (TA) muscle architecture, including muscle thickness (MT), cross‐sectional area (CSA), pennation angle (PA), and fascicle length (FL), to strength and ankle function was examined in ambulatory individuals with CP and unilateral foot drop. Methods: Twenty individuals with CP participated in muscle ultrasound imaging, unilateral strength testing, and three‐dimensional gait analysis. Results: Muscle size (MT and CSA) was positively related to strength, fast gait velocity, and ankle kinematics during walking. Higher PA was related to a more dorsiflexed ankle position at initial contact and inversely with fast gait velocity. FL was related to strength, fast velocity, and step length at a self‐selected speed. Conclusions: Muscle architecture partially explains the degree of impairment in strength and ankle function in CP. Treatments to increase TA size and strength may produce some gait improvement, but other factors that may contribute to ankle performance deficits must be considered. Muscle Nerve, 2011