bluuebunny/crossref_metadata_2025_split huggingface dataset:
Agronomical factors influencing the legumin/vicilin ratio in pea (Pisum sativum L.) seeds
Authors: Mertens Cécile , Dehon Lydie , Bourgeois Audrey , Verhaeghe‐Cartrysse Christine , Blecker Christophe 
Published: December 2011

AbstractBACKGROUND: Many research studies have investigated the impact of agronomical factors on the protein content of pea (Pisum sativum). This study aimed to establish if a correlation exists between protein content and legumin/vicilin (L/V) ratio in pea seeds and to identify agronomical factors that have an impact on this ratio.RESULTS: The L/V ratio was positively correlated with protein content (r = 0.58, P⩽0.01), but no linear regression was applicable (adjusted R2 = 0.31). Both variety and cultivation year had a highly significant effect on the ratio (P < 0.001). The interaction between these two factors was also highly significant (P < 0.001), some varieties being less sensitive to climatic conditions than others. Cultivation location had a highly significant effect (P < 0.01). There was no interaction between variety and location. Seeding density had a highly significant effect on the ratio (P < 0.01), with a saturation effect above 60 seeds m−2.CONCLUSION: Further studies should establish if a linear regression model can be applied to pea varieties independently. Varieties with a stable L/V ratio can prove to be useful in the food industry. Other agronomical factors (soil type and seeding density) should be considered for the production of pea seeds with a specific L/V ratio. Copyright © 2011 Society of Chemical Industry
Monitoring Svalbard rock ptarmigan: Distance sampling and occupancy modeling
Authors: Pedersen Åshild Ø. , Bårdsen Bård‐Jørgen , Yoccoz Nigel G. , Lecomte Nicolas , Fuglei Eva 
Published: December 2011

AbstractThe only resident terrestrial herbivorous bird species in high‐Arctic Svalbard, Norway is the endemic Svalbard rock ptarmigan (Lagopus muta hyperborea) of which little is known of its population dynamics. We assessed temporal and spatial variability of the pre‐breeding population of Svalbard rock ptarmigan males using: 1) distance sampling to estimate density (2000–2009) and 2) occupancy modeling to determine the proportion of survey points being occupied in relation to a habitat index for ptarmigan habitat suitability (2005–2009). Data were collected using a point‐transect sampling design. We split the analysis according to type of survey point (non‐random, random, and survey points combined). Our estimated spring densities were low (1.3–3.1 territorial male/km2, non‐random survey points, 2000–2009) with limited annual variability. The best models describing occupancy rates of territorial males at 2 different spatial scales (ptarmigan males observed ≤250 m and ≤450 m from the sampling point) were independent of spatial scales and the type of survey points. Occupancy dynamics were related to the habitat index whereas detection probability was year dependent. Extinction probability was negatively related to habitat quality (good habitats had lower extinction probability). We could not estimate the habitat effect on colonization precisely because initial occupancy rates were high at both spatial scales (estimated average initial occupancy at scale ≤250 m = 0.96; scale ≤450 m = 0.97). Colonization appeared to be positively related to the habitat index for the random survey points (including mainly marginal habitats), but the small sample size led to large uncertainty in the parameter estimate. Detection probabilities varied greatly between study years, thus demonstrating the importance of estimating detection probability annually. We recommend that future surveys are stratified with respect to habitat quality and to integrate the 2 methodologies in population monitoring of Svalbard rock ptarmigan. © 2011 The Wildlife Society.
Selection of nest‐site habitat by interior least terns in relation to sandbar construction
Authors: Sherfy Mark H. , Stucker Jennifer H. , Buhl Deborah A. 
Published: December 2011

AbstractFederally endangered interior least terns (Sternula antillarum) nest on bare or sparsely vegetated sandbars on midcontinent river systems. Loss of nesting habitat has been implicated as a cause of population declines, and managing these habitats is a major initiative in population recovery. One such initiative involves construction of mid‐channel sandbars on the Missouri River, where natural sandbar habitat has declined in quantity and quality since the late 1990s. We evaluated nest‐site habitat selection by least terns on constructed and natural sandbars by comparing vegetation, substrate, and debris variables at nest sites (n = 798) and random points (n = 1,113) in bare or sparsely vegetated habitats. Our logistic regression models revealed that a broader suite of habitat features was important in nest‐site selection on constructed than on natural sandbars. Odds ratios for habitat variables indicated that avoidance of habitat features was the dominant nest‐site selection process on both sandbar types, with nesting terns being attracted to nest‐site habitat features (gravel and debris) and avoiding vegetation only on constructed sandbars, and avoiding silt and leaf litter on both sandbar types. Despite the seemingly uniform nature of these habitats, our results suggest that a complex suite of habitat features influences nest‐site choice by least terns. However, nest‐site selection in this social, colonially nesting species may be influenced by other factors, including spatial arrangement of bare sand habitat, proximity to other least terns, and prior habitat occupancy by piping plovers (Charadrius melodus). We found that nest‐site selection was sensitive to subtle variation in habitat features, suggesting that rigor in maintaining habitat condition will be necessary in managing sandbars for the benefit of least terns. Further, management strategies that reduce habitat features that are avoided by least terns may be the most beneficial to nesting least terns. © 2011 The Wildlife Society.
Linking alternative food sources to winter habitat selection of herbivores in overbrowsed landscapes
Authors: Massé Ariane , Côté Steeve D. 
Published: December 2011

AbstractDuring winter, ungulates in boreal forests must cope with high energetic costs related to locomotion in deep snow and reduced forage abundance and quality. At high density, ungulates face additional constraints, because heavy browsing reduces availability of woody browse, the main source of forage during winter. Under these severe conditions, large herbivores might forage on alternative food sources likely independent of browsing pressure, such as litterfall or windblown trees. We investigated the influence of alternative food sources on winter habitat selection, by studying female white‐tailed deer (Odocoileus virginianus) living in 2 landscapes with contrasted browse abundance, recently logged and regenerated landscapes, in a population at high density and on a large island free of predators. We fitted 21 female white‐tailed deer with Global Positioning System (GPS) collars and delineated winter home ranges and core areas. We measured snow conditions in different habitat categories and sampled vegetation in the core areas and in the rest of the home ranges to determine how forage abundance, protective cover, and snow conditions influenced habitat selection within the home range. In both landscapes, deer were less likely to use open habitat categories as snow accumulated on the ground. At a finer scale, deer inhabiting the regenerated landscape intensively used areas where balsam fir cover was intermediate with greater balsam fir browse density than in the rest of the home range. In the recently logged landscape, deer were more likely to be found near edges between clear‐cuts and balsam fir stands and in areas where windblown balsam fir trees were present; the latter being the most influential variable. Although balsam fir browse was sparse and mainly out of reach in this landscape, deer increased the use of areas where it was present. Our results offer novel insights into the resource selection processes of northern ungulates, as we showed that access to winter forage, such as woody browse and alternative food sources, depends on climatic conditions and stochastic events, such as abundant compacted snow or windthrows. To compensate for these scarce and unpredictable food supplies, deer selected habitat categories, but mostly areas within those habitat categories, where the likelihood of finding browse, litterfall, and windblown trees was greatest. © 2011 The Wildlife Society.
Trends in volume migration chronology in spring staging Pacific black brant
Authors: Smith Barry D. , Hagmeier Katherine R. , Boyd W. Sean , Dawe Neil K. , Martin Terri D. , Monty Guy L. 
Published: December 2011

AbstractWe used a robust dataset of count and mark‐resighting data for Pacific black brant from 1989–2004, and a novel mark‐recapture model capable of analyzing such data, to calculate the annual variability and timing of brant as they migrated through the Parksville–Qualicum Beach area, a traditional spring staging site in coastal British Columbia, Canada. Our analysis indicated that the date of departure from this site to northern breeding sites advanced between 10 and 20 days over this period because of a combination of earlier arrival and shorter residence times. Given this change in migration behavior, and the potential implications for population dynamics, we recommend that targeted research on brant wintering, migration, and reproductive strategies should be examined within a greater Pacific‐wide context. In this way, the consequences of proximate factors (e.g., disturbance, food, and climate) can be understood in terms of individual fitness and population dynamics. Finally, at the local level, conservation actions are needed to ensure the long term sustainability of Parksville–Qualicum Beach as an important spring staging site for Pacific black brant. © 2011 The Wildlife Society.
Creating the owner's mindset
Authors: Prosek Jennifer 
Published: December 2011

AbstractAll employees, not just leaders, must cultivate the mindset of an owner, Prosek asserts. Others besides you must maintain the mentality that they too have a financial stake. She describes the “Commission for Life” that has been successful in her own company. Anyone can earn a 5 percent commission (as long as they remain with the firm) on new business that has been brought in as the result of setting up a successful new business meeting. This device, Prosek finds, keeps staffers inspired, motivated, and connected.
Photocrosslinkable Polyesters and Poly(ester anhydride)s for Biomedical Applications
Authors: Seppälä Jukka , Korhonen Harri , Hakala Risto , Malin Minna 
Published: December 2011

AbstractCrosslinking is a feasible way to prepare biodegradable polymers with potential in biomedical applications such as controlled release of active agents and tissue engineering. A synthesis route in which functional telechelic aliphatic polyester oligomers are used as precursors for the preparation of crosslinked polyesters and poly(ester anhydride)s is described. Mechanical properties, degradation characteristics and rate, and bioactivity can be modified widely by controlling the chemical composition and architecture of the crosslinkable oligomers. In tissue engineering, photocrosslinking allows to use crosslinkable oligomers in advanced manufacturing techniques like micromolding in capillaries, stereolithography and two‐photon polymerization.magnified image
Biodegradable Polymers Derived From Amino Acids
Authors: Khan Wahid , Muthupandian Saravanan , Farah Shady , Kumar Neeraj , Domb Abraham J. 
Published: December 2011

AbstractIn the past three decades, the use of polymeric materials has increased dramatically for biomedical applications. Many α‐amino acids derived biodegradable polymers have also been intensely developed with the main goal to obtain bio‐mimicking functional biomaterials. Polymers derived from α‐amino acids may offer many advantages, as these polymers: (a) can be modified further to introduce new functions such as imaging, molecular targeting and drugs can be conjugated chemically to these polymers, (b) can improve on better biological properties like cell migration, adhesion and biodegradability, (c) can improve on mechanical and thermal properties and (d) their degradation products are expected to be non‐toxic and readily metabolized/excreted from the body. This manuscript focuses on biodegradable polymers derived from natural amino acids, their synthesis, biocompatibility and biomedical applications. It is observed that polymers derived from α‐amino acids constitute a promising family of biodegradable materials. These provide innovative multifunctional polymers possessing amino acid side groups with biological activity and with innumerous potential applications.magnified image
Corrosion under mixed flowing gas conditions of various connector coatings
Authors: Collins M. N. , Reid M. , Dalton E. , Shannon K. , Garfias‐Mesias L. F. 
Published: December 2011

AbstractThis paper presents an investigation on the corrosion performance of Ni/Au, NiP/Au, Ni/NiP/Au and Ni/NiPd/Au electrical connector coating systems. The coating systems were exposed to 10 days Class III mixed flowing gas and were subsequently examined by X‐ray diffraction, focused ion beam microscopy, scanning electron microscopy and energy dispersive spectroscopy to evaluate the performance of each coating system. The Ni/Au coating system showed the worst performance followed by NiP/Au and Ni/NiP/Au. The Ni/NiPd/Au coated connector materials exhibited the least surface corrosion and this was attributed to a number of factors including a thicker coating system, more compact gold layer with fewer defects and a reduction in the electrochemical potential difference between layers reducing the local cell effect.
Species‐specific stable isotope analysis by the hyphenation of chromatographic techniques with MC‐ICPMS
Authors: Rodríguez‐González Pablo , Epov Vladimir N. , Pecheyran Christophe , Amouroux David , Donard Olivier F.X. 
Published: December 2011

AbstractThis work reviews the basis and all the existing publications on the hyphenation of chromatography‐based techniques to MC‐ICPMS for isotopic studies that were published until the end of 2010. A brief historical retrospective of the measurement of isotope ratios from transient signals by ICPMS with different sample introduction techniques is also included. The most important experimental parameters and data reduction strategies affecting the accurate and precise measurement of compound‐specific isotope ratios by either HPLC or GC coupled to MC‐ICPMS are discussed. All the applications are reported and critically reviewed in terms of analytical characteristics, performances, optimization, advantages and disadvantages and future applicability to the environmental, geochemical, or bioinorganic studies. © 2011 Wiley Periodicals, Inc. Mass Spec Rev 31:504–521, 2012
Possible involvement of Wnt11 in colorectal cancer progression
Authors: Nishioka Mitsuaki , Ueno Koji , Hazama Shoichi , Okada Toshiyuki , Sakai Kouhei , Suehiro Yutaka , Okayama Naoko , Hirata Hiroshi , Oka Masaaki , Imai Kohzoh , Dahiya Rajvir , Hinoda Yuji 
Published: December 2011

AbstractOur previous report revealed that the expression of Frizzled‐7 (FZD7) in colorectal cancer (CRC) and its possible role in CRC progression. In this study we measured the expression levels of candidate FZD7 ligands, Wnt3 and Wnt11 in colon cancer cell lines (n = 7) and primary CRC tissues (n = 133) by quantitative RT‐PCR. We also examined the functional effects of Wnt11 with the use of Wnt11 transfectants of colon cancer HCT‐116 cells. Wnt11 transfectants showed the increased proliferation and migration/invasion activities compared to mock cells. Western blot analysis of transfectants revealed that phosphorylation of JNK and c‐jun was increased after Wnt11 transfection. Wnt11 mRNA expression was significantly higher in the stage I, II, III, or IV tumor tissues than in non‐tumor tissues (overall P < 0.003), while there was no significant difference in Wnt3 mRNA expression between tumor and non‐tumor tissues. In addition, Wnt11 mRNA expression was significantly higher in patients with recurrence or death after surgery than in those with no recurrence (disease free) after surgery (P = 0.018). We also compared the expression levels of Wnt11 mRNA with those of FZD7 mRNA in the same CRC samples. Wnt11 mRNA expression was significantly higher in patients with higher FZD7 mRNA levels than in those with lower FZD7 mRNA levels (P = 0.0005). The expression levels of Wnt11 mRNA were correlated with those of FZD7 mRNA (P < 0.0001). These data suggest that Wnt11 may play an important role in CRC progression. © 2011 Wiley Periodicals, Inc.
Polycomb group protein EZH2‐mediated E‐cadherin repression promotes metastasis of oral tongue squamous cell carcinoma
Authors: Wang Cheng , Liu Xiqiang , Chen Zujian , Huang Hongzhang , Jin Yi , Kolokythas Antonia , Wang Anxun , Dai Yang , Wong David T.W. , Zhou Xiaofeng 
Published: December 2011

AbstractEnhancer of Zeste homolog 2 (EZH2) is a critical component of the polycomb‐repressive complex 2 (PRC2) that regulates many essential biological processes, including embryogenesis and many developmental events. The oncogenic role of EZH2 has recently been implicated in several cancer types. In this study, we first confirmed that the over‐expression of EZH2 is a frequent event in oral tongue squamous cell carcinoma (OTSCC). We further demonstrated that EZH2 over‐expression is correlated with advanced stages of the disease and is associated with lymph node metastasis. Statistical analysis revealed that EZH2 over‐expression was correlated with reduced overall survival. Furthermore, over‐expression of EZH2 was correlated with reduced expression of tumor suppressor gene E‐cadherin. These observations were confirmed in vitro, in which knockdown of EZH2‐induced E‐cadherin expression and reduced cell migration and invasion. In contrast, ectopic transfection of EZH2 led to reduced E‐cadherin expression and enhanced cell migration and invasion. Furthermore, EZH2 may act on cell migration in part by suppressing the E‐cadherin expression. Taken together, these data suggest that EZH2 plays major roles in the progression of OTSCC, and may serve as a biomarker or therapeutic target for patients at risk of metastasis. © 2011 Wiley Periodicals, Inc.
Association of the miR‐146aC>G, 149C>T, 196a2C>T, and 499A>G polymorphisms with colorectal cancer in the Korean population
Authors: Min Kyung Tae , Kim Jong Woo , Jeon Young Joo , Jang Moon Ju , Chong So Young , Oh Doyeun , Kim Nam Keun 
Published: December 2011

AbstractMicroRNAs (miRNAs) are small, 18‐ to 22‐nucleotide non‐coding RNAs that regulate target gene expression. Although recent studies focused on various diseases that harbor the miR‐146aC>G (rs2910164), 149C>T (rs2292832), 196a2C>T (rs11614913), and 499A>G (rs3746444) polymorphisms, the role of miRNA genetic variants in colorectal cancer is still unknown. The present study aimed to evaluate the role of four miRNA polymorphisms in patients with colorectal cancer. We enrolled 446 colorectal cancer patients and 502 control subjects from the Korean population. We found a significantly increased colorectal cancer risk with the miR‐196a2CC genotype compared with the TT/CT genotype (AOR = 1.50; 95% CI = 1.11–2.04; P = 0.01; FDR‐P = 0.04). In the stratified analyses, we observed both weak and strong association data. We found stronger associations of the miR‐196a2 variants in the non‐diabetic and rectal cancer groups than other stratified groups. Our data suggest that the miRNA variants could affect the development of colorectal cancer in the Korean population. © 2011 Wiley Periodicals, Inc.
Lysophosphatidic acid receptor‐3 increases tumorigenicity and aggressiveness of rat hepatoma RH7777 cells
Authors: Okabe Kyoko , Hayashi Mai , Kato Kohei , Okumura Mai , Fukui Rie , Honoki Kanya , Fukushima Nobuyuki , Tsujiuchi Toshifumi 
Published: December 2011

AbstractLysophosphatidic acid (LPA), which interacts with G protein‐coupled transmembrane LPA receptors exhibits several biological effects, such as cell proliferation, migration, and differentiation. Recently, it has been reported that alteration of LPA receptor genes occurs in several cancer cells. In this study, to assess the biological role of LPA receptor‐3 (LPA3) in the pathogenesis of tumor cells, we generated the Lpar3‐expressing cells (RHa3B12 and RHa3G8) from rat hepatoma RH7777 cells, and examined their abilities of cell migration and tumorigenicity, compared with the Lpar3‐unexpressing cells. In cell motility and invasion assays, RHa3B12 and RHa3G8 cells showed significantly higher intrinsic activity without LPA treatment than control RH7777AB cells. LPA treatment further increased cell motility and invasion of these cells. The cell motility of RHa3B12 and RHa3G8 cells stimulated by LPA treatment was significantly suppressed by pretreatment with inhibitors of Gi or Gq proteins. In a soft agar assay, the large sized colonies were formed in RHa3B12 and RHa3G8 cells, but not in RH7777AB cells. The cell survival of RHa3G8 cells treated with cisplatin (CDDP) or doxorubicin (DOX) was higher than that of RH7777AB cells, correlating with the elevated expression levels of multidrug‐resistance related genes, Mdr1a, Mdr1b, and Gstp1. These results suggest that LPA3 may be involved in progression and aggressiveness of rat hepatoma RH7777 cells. © 2011 Wiley Periodicals, Inc.
Overexpression of Evi‐1 oncoprotein represses TGF‐β signaling in colorectal cancer
Authors: Deng Xiyun , Cao Yanna , Liu Yan , Li Fazhi , Sambandam Kamalanathan , Rajaraman Srinivasan , Perkins Archibald S. , Fields Alan P. , Hellmich Mark R. , Townsend Courtney M. , Thompson E. Aubrey , Ko Tien C. 
Published: December 2011

AbstractHuman colorectal cancer (CRC) cells are resistant to the anti‐proliferative effect of transforming growth factor‐β (TGF‐β), suggesting that disruption of TGF‐β signaling plays an important role in colorectal carcinogenesis. Ecotropic virus integration site‐1 (Evi‐1) oncoprotein represses TGF‐β signaling by interacting with Smads, but its role in CRC has not been established. The purpose of this study is to determine whether Evi‐1 plays role(s) in CRCs and to characterize Evi‐1 transcript(s) in CRCs. Evi‐1 was overexpressed in 53% of human CRC samples, 100% of colon adenoma samples, and 100% of human colon cancer cell lines tested. Using 5′ RACE, we cloned a novel Evi‐1 transcript (Evi‐1e) from a human CRC tissue and found that this novel transcript was expressed at a higher level in CRC tissues than in normal tissues and was the major Evi‐1 transcript in CRCs. Transient Evi‐1 transfection inhibited TGF‐β‐induced transcriptional activity and reversed the growth inhibitory effect of TGF‐β in MC‐26 mouse colon cancer cells. In conclusion, we have identified overexpression of Evi‐1 oncoprotein as a novel mechanism by which a subset of human CRCs may escape TGF‐β regulation. We have also identified a novel Evi‐1 transcript, Evi‐1e, as the major Evi‐1 transcript expressed in human CRCs. © 2011 Wiley Periodicals, Inc.