bluuebunny/crossref_metadata_2025_split huggingface dataset:
The trichothecene mycotoxin deoxynivalenol facilitates cell‐to‐cell invasion during wheat‐tissue colonization by Fusarium graminearum
Authors: Armer Victoria J. , Urban Martin , Ashfield Tom , Deeks Michael J. , Hammond‐Kosack Kim E. 
Published: June 2024

AbstractFusarium head blight disease on small‐grain cereals is primarily caused by the ascomycete fungal pathogen Fusarium graminearum. Infection of floral spike tissues is characterized by the biosynthesis and secretion of potent trichothecene mycotoxins, of which deoxynivalenol (DON) is widely reported due to its negative impacts on grain quality and consumer safety. The TRI5 gene encodes an essential enzyme in the DON biosynthesis pathway and the single gene deletion mutant, ΔTri5, is widely reported to restrict disease progression to the inoculated spikelet. In this study, we present novel bioimaging evidence revealing that DON facilitates the traversal of the cell wall through plasmodesmata, a process essential for successful colonization of host tissue. Chemical complementation of ΔTri5 did not restore macro‐ or microscopic phenotypes, indicating that DON secretion is tightly regulated both spatially and temporally. A comparative qualitative and quantitative morphological cellular analysis revealed infections had no impact on plant cell wall thickness. Immunolabelling of callose at plasmodesmata during infection indicates that DON can increase deposits when applied exogenously but is reduced when F. graminearum hyphae are present. This study highlights the complexity of the interconnected roles of mycotoxin production, cell wall architecture and plasmodesmata in this highly specialized interaction.
Adaptive substitutions at two amino acids of HCPro modify its functional properties to separately increase the virulence of a potyviral chimera
Authors: Sun Hao , Ciska Malgorzata , Makki Mongia , Tenllado Francisco , Canto Tomás 
Published: June 2024

AbstractWe had previously reported that a plum pox virus (PPV)‐based chimera that had its P1‐HCPro bi‐cistron replaced by a modified one from potato virus Y (PVY) increased its virulence in some Nicotiana benthamiana plants, after mechanical passages. This correlated with the natural acquisition of amino acid substitutions in several proteins, including in HCPro at either position 352 (Ile→Thr) or 454 (Leu→Arg), or of mutations in non‐coding regions. Thr in position 352 is not found among natural potyviruses, while Arg in 454 is a reversion to the native PVY HCPro amino acid. We show here that both mutations separately contributed to the increased virulence observed in the passaged chimeras that acquired them, and that Thr in position 352 is no intragenic suppressor to a Leu in position 454, because their combined effects were cumulative. We demonstrate that Arg in position 454 improved HCPro autocatalytic cleavage, while Thr in position 352 increased its accumulation and the silencing suppression of a reporter in agropatch assays. We assessed infection by four cloned chimera variants expressing HCPro with none of the two substitutions, one of them or both, in wild‐type versus DCL2/4‐silenced transgenic plants. We found that during infection, the transgenic context of altered small RNAs affected the accumulation of the four HCPro variants differently and hence, also infection virulence.
Candida albicans skin infection in diabetic patients: An updated review of pathogenesis and management
Authors: Shahabudin Sakina , Azmi Nina Suhaity , Lani Mohd Nizam , Mukhtar Muhammad , Hossain Md. Sanower 
Published: June 2024

AbstractCandida species, commensal residents of human skin, are recognized as the cause of cutaneous candidiasis across various body surfaces. Individuals with weakened immune systems, particularly those with immunosuppressive conditions, are significantly more susceptible to this infection. Diabetes mellitus, a major metabolic disorder, has emerged as a critical factor inducing immunosuppression, thereby facilitating Candida colonization and subsequent skin infections. This comprehensive review examines the prevalence of different types of Candida albicans‐induced cutaneous candidiasis in diabetic patients. It explores the underlying mechanisms of pathogenicity and offers insights into recommended preventive measures and treatment strategies. Diabetes notably increases vulnerability to oral and oesophageal candidiasis. Additionally, it can precipitate vulvovaginal candidiasis in females, Candida balanitis in males, and diaper candidiasis in young children with diabetes. Diabetic individuals may also experience candidal infections on their nails, hands and feet. Notably, diabetes appears to be a risk factor for intertrigo syndrome in obese individuals and periodontal disorders in denture wearers. In conclusion, the intricate relationship between diabetes and cutaneous candidiasis necessitates a comprehensive understanding to strategize effective management planning. Further investigation and interdisciplinary collaborative efforts are crucial to address this multifaceted challenge and uncover novel approaches for the treatment, management and prevention of both health conditions, including the development of safer and more effective antifungal agents.
PsSOC1 is involved in the gibberellin pathway to trigger cell proliferation and budburst during endodormancy release in tree peony
Authors: Zhang Yuxi , Niu Demei , Yuan Yanchao , Liu Fang , Wang Zhiwei , Gao Linqiang , Liu Chunying , Zhou Gongke , Gai Shupeng 
Published: June 2024

Summary

Tree peony (Paeonia suffruticosa) undergoes bud endodormancy, and gibberellin (GA) pathway plays a crucial role in dormancy regulation. Recently, a key DELLA protein PsRGL1 has been identified as a negative regulator of bud dormancy release. However, the mechanism of GA signal to break bud dormancy remains unknown.

In this study, yeast two‐hybrid screened PsSOC1 interacting with PsRGL1 through its MADS domain, and interaction was identified using pull‐down and luciferase complementation imaging assays Transformation in tree peony and hybrid poplar confirmed that PsSOC1 facilitated bud dormancy release. Transcriptome analysis of PsSOC1‐overexpressed buds indicated PsCYCD3.3 and PsEBB3 were its potential downstream targets combining with promoter survey, and they also accelerated bud dormancy release verified by genetic analysis.

Yeast one‐hybrid, electrophoretic mobility shifts assays, chromatin immunoprecipitation quantitative PCR, and dual luciferase assays confirmed that PsSOC1 could directly bind to the CArG motif of PsCYCD3.3 and PsEBB3 promoters via its MADS domain. PsRGL1–PsSOC1 interaction inhibited the DNA‐binding activity of PsSOC1. Additionally, PsCYCD3.3 promoted bud dormancy release by rebooting cell proliferation.

These findings elucidated a novel GA pathway, GA‐PsRGL1‐PsSOC1‐PsCYCDs, which expanded our understanding of the GA pathway in bud dormancy release.
A bacterial effector manipulates plant metabolism, cell death, and immune responses via independent mechanisms
Authors: Zhao Achen , Xian Liu , Franco Ortega Sara , Yu Gang , Macho Alberto P. 
Published: June 2024

Summary

Bacterial pathogens inject effector proteins inside plant cells to manipulate cellular functions and achieve a successful infection. The soil‐borne pathogen Ralstonia solanacearum (Smith), the causal agent of bacterial wilt disease, secretes > 70 different effectors inside plant cells, although only a handful of them have been thoroughly characterized. One of these effectors, named RipI, is required for full R. solanacearum pathogenicity. RipI associates with plant glutamate decarboxylases (GADs) to promote the accumulation of gamma‐aminobutyric acid (GABA), which serves as bacterial nutrient.

In this work, we found that RipI can also suppress plant immune responses to bacterial elicitors, which seems to be unrelated to the ability of RipI to induce GABA accumulation and plant cell death.

A detailed characterization of the RipI features that contribute to its virulence activities identified two residues at the C‐terminal domain that mediate RipI interaction with plant GADs and the subsequent promotion of GABA accumulation. These residues are also required for the appropriate homeostasis of RipI in plant cells and the induction of cell death, although they are partially dispensable for the suppression of plant immune responses.

Altogether, we decipher and uncouple the virulence activities of an important bacterial effector at the biochemical level.
The RXLR effector PpE18 of Phytophthora parasitica is a virulence factor and suppresses peroxisome membrane‐associated ascorbate peroxidase NbAPX3‐1‐mediated plant immunity
Authors: Cao Yimeng , Zhang Qiang , Liu Yuan , Yan Tiantian , Ding Liwen , Yang Yang , Meng Yuling , Shan Weixing 
Published: June 2024

Summary

Phytophthora parasitica causes diseases on a broad range of host plants. It secretes numerous effectors to suppress plant immunity. However, only a few virulence effectors in P. parasitica have been characterized.

Here, we highlight that PpE18, a conserved RXLR effector in P. parasitica, was a virulence factor and suppresses Nicotiana benthamiana immunity. Utilizing luciferase complementation, co‐immunoprecipitation, and GST pull‐down assays, we determined that PpE18 targeted NbAPX3‐1, a peroxisome membrane‐associated ascorbate peroxidase with reactive oxygen species (ROS)‐scavenging activity and positively regulates plant immunity in N. benthamiana. We show that the ROS‐scavenging activity of NbAPX3‐1 was critical for its immune function and was hindered by the binding of PpE18. The interaction between PpE18 and NbAPX3‐1 resulted in an elevation of ROS levels in the peroxisome.

Moreover, we discovered that the ankyrin repeat‐containing protein NbANKr2 acted as a positive immune regulator, interacting with both NbAPX3‐1 and PpE18. NbANKr2 was required for NbAPX3‐1‐mediated disease resistance. PpE18 competitively interfered with the interaction between NbAPX3‐1 and NbANKr2, thereby weakening plant resistance.

Our results reveal an effective counter‐defense mechanism by which P. parasitica employed effector PpE18 to suppress host cellular defense, by suppressing biochemical activity and disturbing immune function of NbAPX3‐1 during infection.
MoLO: Drift‐free lidar odometry using a 3D model
Authors: Zhao H. , Zhao Y. , Tomko M. , Khoshelham K. 
Published: June 2024

AbstractLiDAR odometry enables localising vehicles and robots in the environments where global navigation satellite systems (GNSS) are not available. An inherent limitation of LiDAR odometry is the accumulation of local motion estimation errors. Current approaches heavily rely on loop closure to optimise the estimated sensor poses and to eliminate the drift of the estimated trajectory. Consequently, these systems cannot perform real‐time localization and are therefore not practical for a navigation task. This paper presents MoLO, a novel model‐based LiDAR odometry approach to achieve real‐time and drift‐free localization using a 3D model of the environment containing planar surfaces, namely the structural elements of buildings. The proposed approach uses a 3D model of the environment to initialise the LiDAR pose and includes a scan‐to‐scan registration to estimate the pose for consecutive LiDAR scans. Re‐registering LiDAR scans to the 3D model at a certain frequency provides the global sensor pose and eliminates the drift of the trajectory. Pose graphs are built frequently to acquire a smooth and accurate trajectory. A geometry‐based method and a learning‐based method to register LiDAR scans with the 3D model are tested and compared. Experimental results show that MoLO can eliminate drift and achieve real‐time localization while providing an accuracy equivalent to loop closure optimization.
The effect of reminiscence therapy on cognitive level, quality of life and depressive symptoms in older adults with type 2 diabetes: a randomised controlled trial
Authors: Selçuk Tosun Alime , Lök Neslihan , Duran Büşra , Akgul Gundogdu Nurcan 
Published: June 2024

AbstractBackgroundEvidence suggests that reminiscence therapy can effectively reduce cognitive and psychological symptoms. This study was conducted to examine the effect of individual reminiscence therapy on cognitive level, quality of life and depressive symptoms in older adults with type 2 diabetes.MethodsThis study is an experimental type, randomised controlled study designed in pre‐test – post‐test design. The sample size of the study consisted of 58 older adults with type 2 diabetes, consisting of 29 intervention and 29 control subjects. The older adults in the intervention group were individually subjected to 5 weeks of reminiscence therapy in two sessions (each session 40–45 min) per week. No intervention was applied to the control group. Personal Information Form, Standardised Mini Mental Test, SF‐36 Quality of Life and Beck Depression Inventory were used to collect the data.ResultsWhile there was no significant difference between the pre‐test Mini Mental Test, Beck Depression Inventory total score and SF‐36 Quality of Life Scale mental and physical health sub‐dimension mean scores of the older adults in the intervention group and the mean scores of those in the control group (P > 0.05), the difference between the Mini Mental Test, Beck Depression Inventory total score and SF‐36 Quality of Life Scale mental and physical health sub‐dimension mean scores of the older adults in the intervention and control groups in the post‐test was found to be statistically significant (P < 0.05). The post‐test Mini Mental Test total score, SF‐36 Quality of Life Scale mental and physical health sub‐dimension mean scores of the older adults in the intervention group were higher than the older adults in the control group. It was determined that the post‐test Beck Depression Inventory total mean scores of the older adults in the intervention group were lower than the older adults in the control group.ConclusionReminiscence therapy can positively affect cognitive levels, quality of life and depression symptoms of older adults living in the community. Individual reminiscence therapy can be used as a nursing intervention in primary healthcare.
Quality and fertilizing ability of frozen–thawed porcine sperm separated using a migration sedimentation method
Authors: Nguyen Suong Thi , Taniguchi Masayasu , Ono Tetsushi , Takagi Mitsuhiro , Lin Qingyi , Torigoe Nanaka , Liu Bin , Namula Zhao , Nagahara Megumi , Otoi Takeshige 
Published: June 2024

AbstractWe evaluated the quality and fertilizing ability of frozen–thawed porcine sperm that were selected using a commercially available device (MIGLIS, Menicon Life Science) consisting of three parts: an outer lid, an inner lid, and a tube. Firstly, to determine an adequate concentration of caffeine for separation, frozen–thawed sperm were incubated with different concentrations of caffeine (0, 1, 2.5, 5, and 10 mM) in a MIGLIS device. To determine the appropriate incubation time for separating sperm in the MIGLIS device, frozen–thawed sperm were incubated with 2.5 mM caffeine for 5, 10, 15, or 20 min. To evaluate the fertilization and embryo development of oocytes fertilized with frozen–thawed sperm separated into two regions (outer and inner) in the MIGLIS device, the separated sperm from the three boars was used to fertilize in vitro‐matured oocytes and cultured in vitro for 7 days. Sperm quality parameters of sperm collected from the inner tube after incubation with 2.5 mM caffeine were superior to sperm incubated without caffeine. Moreover, sperm collected from the inner tube after incubation for 10 min had a higher progressive motility. The rate of blastocyst produced from spermatozoa collected from the inner tube after incubation with 2.5 mM caffeine for 10 min significantly increased compared to that produced from spermatozoa from the outer tube, regardless of the boar. In conclusion, sperm sorting using the MIGLIS device may be useful for separating high‐quality sperm after incubation with 2.5 mM caffeine for 10 min to improve blastocyst formation.
The role of energy communities for thermal networks: An EU legal perspective
Authors: Cocciolo Endrius 
Published: June 2024

AbstractDecarbonising heating and cooling systems, significant contributors to the EU's (European Union) energy consumption, is a complex and urgent matter. This article discusses the potential coupling of energy communities and district heating and cooling systems, particularly fourth‐ and fifth‐generation models, in the local energy transition. The integration of new‐generation district heating and cooling systems into renewable energy generation is crucial for sustainability, circular economy practices, and community‐based solutions. An examination of the EU legal framework within the Fit‐for‐55 package elucidates how provisions from the new Energy Efficiency Directive and Renewable Energy Directive facilitate the implementation of energy communities in the heating and cooling sector. The existing enabling legislation underscores the suitability of energy communities for heating and cooling sector activities, acknowledging their transformative potential in advancing the energy transition and fostering community empowerment.
Determinantal approach to multiple orthogonal polynomials and the corresponding integrable equations
Authors: Doliwa Adam 
Published: June 2024

AbstractWe study multiple orthogonal polynomials exploiting their explicit determinantal representation in terms of moments. Our reasoning follows that applied to solve the Hermite–Padé approximation and interpolation problems. We also study families of multiple orthogonal polynomials obtained by variation of the measures known from the theory of discrete‐time Toda lattice equations. We present determinantal proofs of certain fundamental results of the theory, obtained earlier by other authors in a different setting. We also derive quadratic identities satisfied by the polynomials, which are new elements of the theory. Resulting equations allow to present multiple orthogonal polynomials within the theory of integrable systems.
How do OECD countries differ in population ageing and the situation of people aged 65 and older? Evidence using cluster analysis
Authors: Szymańska Agata 
Published: June 2024

AbstractThe study compares the conditions created by ageing processes. It recognises differences in the situation of people aged 65 and older from a macrolevel perspective in 34 OECD countries in 2020, that is, the first year of the Covid‐19 pandemic. The paper analyses the (dis)similarities of OECD economies with regard to a set of selected variables that represent different macrolevel dimensions. Cluster analysis and linear ordering were applied, which made it possible to group the countries into separate clusters. The most obvious difference was found for the cluster consisting of Luxembourg, Belgium, France, and Spain, relative to the group that includes Mexico and Türkiye and the cluster that comprises Korea, Latvia, Japan, and Lithuania. Influential variables for grouping countries were old‐age income poverty, public transfers in the form of pensions, effective labour market exit age, and differences between expected years in retirement for women and men. The cluster analysis for 2020 also revealed the general difference between European countries and the rest of the OECD economies, as well as the importance of regional differences. The results also indicate that the clusters partially correspond to the differentiation of OECD countries based on the typology of the pension regimes, as outlined in the literature. Finally, comparing the 2020 clusters with the pre‐Covid‐19 pandemic results (2018) indicates that countries in the top and bottom‐ranked groups were resistant to the pandemic shock.
Developing a novel inclusive policy analysis framework based on capability approach and institutional analysis and development method
Authors: Biswas Arindam 
Published: June 2024

AbstractThis paper develops a novel framework to measure inclusive policy based on capability approach and institutional analysis and development method. In the process, it records its distinction of inclusive growth from earlier growth theories, particularly highlighting its economic and non‐economic dimensions. The paper inculcates consequential ethics in discussing inclusive growth. The proposed framework is conceptualised on the theoretical foundation of Amartya Sen's capability approach and Elinor Ostrom's IAD method. The framework allows theorists to develop more precise theories and models and derive empirical analysis of existing and proposed policies. The paper identifies the major limitations of the existing inclusive policy analysis methods which include disregarding the ‘noneconomic dimensions of inclusive growth’ (benefit share, participation, equality of opportunity). The existing methods endorses equality of opportunity from an arbitrary point to wrongly fulfil the inclusiveness dimension. Such an approach fails in sustaining moral and ethical rightness of equity. Further, the excising methodologies do not disclose the precise time of change in opportunities among the target population resulting into waste of policy resources. The proposed framework's novel contribution is integrating CA and IAD method to reveal the precise time of change in opportunity that occurs over time due to policy implementation. The framework will help policy makers to measure policy outcome and reduce waste of resource. Analysts and researchers can apply framework for empirical analysis to identify the inclusive growth policy or compare inclusive policy between various countries, states, and regions.
Evaluation of assays for nucleic acid testing for the prevention of chikungunya and dengue virus transmission by blood transfusion
Authors: Gallian Pierre , Dupont Isabelle , Lacoste Marjorie , Brisbarre Nadège , Isnard Christine , Delouane Idriss , Richard Pascale , Morel Pascal , Laperche Syria , de Lamballerie Xavier 
Published: June 2024

AbstractBackgroundThe large dengue (DENV) and chikungunya (CHIKV) outbreaks observed during the last decade across the world, as well as local transmissions in non‐endemic areas are a growing concern for blood safety. The aim of this study was to evaluate and compare the sensitivity of nucleic acid tests (NAT) detecting DENV and CHIKV RNA.Materials and MethodsUsing DENV 1 to 4 International Standards, the limits of detection (LODs) calculated by probit analysis of two NAT assays; the cobas CHIKV/DENV assay (Roche Diagnostics) and the Procleix Dengue Virus Assay (Grifols) were compared. In addition, CHIKV‐RNA LOD of the cobas CHIKV/DENV assay was evaluated.ResultsFor dengue, the 95% LOD of the cobas assay ranged between 4.10 [CI95%: 2.70–8.19] IU/mL (DENV‐2) and 7.07 [CI95%: 4.34–14.89] IU/mL (DENV‐4), and between 2.19 [CI95%: 1.53–3.83] IU/mL (DENV‐3) and 5.84 [CI95%: 3.84–10.77] IU/mL (DENV‐1) for Procleix assay. The Procleix assay had a significant lower LOD for DENV‐3 (2.19 vs. 5.89 IU/mL) when compared to the cobas assay (p = 0.005). The 95% LOD for CHIKV‐RNA detection of the cobas assay was 4.76 [CI95%: 3.08–8.94] IU/mL.DiscussionThe two NAT assays developed for blood donor screening evaluated in this study demonstrated high and similar analytical performance. Subject to an appropriate risk‐benefit assessment, they can be used to support blood safety during outbreaks in endemic areas or in non‐endemic areas as an alternative to deferring blood donors during local transmission likely to affect the blood supply. The development of multiplex assays is expected to optimize laboratory organization.