bluuebunny/crossref_metadata_2025_split huggingface dataset:
Raman spectroscopy monitoring of the cellular activities of a tissue‐engineered ex vivo produced oral mucosal equivalent
Authors: Lo Wen‐Liang , Lai Jian‐Yun , Feinberg Stephen E. , Izumi Kenji , Kao Shou‐Yen , Chang Che‐Shoa , Lin Alan , Chiang Huihua Kenny 
Published: May 2010

AbstractTo ensure quality control and assurance in tissue engineering, noninvasive, real‐time and aseptic evaluation of cell‐based devices is required before product release. In this study, Raman spectroscopy was applied to monitor the cellular activities of an oral mucosa equivalent (EVPOME) produced ex vivo from cultured autogenous oral keratinocytes and acellular dermis—AlloDerm. Raman spectra showed a positive correlation of the peak area ratio of amide I (1655 cm−1)/phenylalanine (1004 cm−1) with a negative linear regression (R2 > 0.95) according to the number of cultured days, especially on the 14thand 21st day. This work demonstrates the successful application of Raman spectroscopy for quantitatively monitoring and evaluating the maturity of EVPOME. Copyright © 2010 John Wiley & Sons, Ltd.
The use of liquid phase deposition prepared phosphonate grafted silica nanoparticle‐deposited capillaries in the enrichment of phosphopeptides
Authors: Wu Jian‐Hong , Zhao Yong , Li Ting , Xu Cong , Xiao Kuang , Feng Yu‐Qi , Guo Lin 
Published: May 2010

AbstractIn our current work, we describe how open tubular‐immobilized metal‐ion affinity chromatography (OT‐IMAC) capillary columns connected to a solid phase microextraction (in‐tube SPME) device can be used for the enrichment of phosphopeptides. A phosphonate modified silica nanoparticle (NP)‐deposited capillary was prepared by liquid phase deposition (LPD), and used for the immobilization of Fe3+, Zr4+ or Ti4+. The enrichment capacities of three different OT‐IMAC capillary columns were compared by using tryptically digested α‐casein as sample. The improved extraction efficiency in our technique was demonstrated by comparing to a directly modified capillary, and a comparison of phosphopeptide extraction from simple and complex samples was tested for both modes. Our results show that the NP‐IMAC‐Zr4+ capillary column can be used to selectively isolate phosphopeptides from real samples, and can enrich for β‐casein phosphopeptides from concentrations as low as 1.7×10−9 M.
Tonsil size as a predictor of cardiac complications in children with sleep‐disordered breathing
Authors: Granzotto Eduardo Homrich , Aquino Flavio Veras , Flores José Antônio , Neto José Faibes Lubianca 
Published: May 2010

AbstractObjectives/Hypothesis:The primary objective was to evaluate the association of palatine (T/P) tonsil size determined by radiography with pulmonary artery pressure (PAP) measured by Doppler echocardiography in children with surgical indication for adenotonsillar hypertrophy. The secondary objective was to evaluate if tonsil size could help to identify children at higher risk of pulmonary artery hypertension (PAH).Study Design:Cross‐sectional study.Methods:The study was conducted with a consecutive sample of children with indication of adenotonsillectomy for sleep‐disordered breathing. T/P was measured by lateral neck radiography, PAP by echodopplercardiography, and symptoms and quality of life by the Obstructive Sleep Apnea (OSA)‐18 questionnaire. T/P was plotted in a receiver operating characteristic (ROC) curve to determine the best cut‐off point to identify children with PAH.Results:A total of 45 children participated in the study. The mean age was 72.0 ± 32.3 months, and six (13%) patients had PAH. Correlation between systolic PAP and T/P was strong (r = 0.624; P < .0001). T/P was significantly higher in patients with PAH (P < .001). OSA‐18 score did not significantly correlate with the variables. The cut‐off point identified in the ROC was 0.66, which was the minimum T/P where sensitivity was still 100%. Mean systolic pulmonary artery pressure in children with T/P >0.66 was significantly higher than in those with T/P <0.66 (P < .001).Conclusions:T/P showed a good correlation with PAP in children with adenotonsillar hypertrophy and surgical indication for sleep‐disordered breathing. Children with T/P >0.66 can be at greater risk for cardiac complications and should be submitted to complementary studies with echodopplercardiography or given preference for surgery.
The role of intratympanic lactate injection in the prevention of cisplatin‐induced ototoxicity
Authors: Nader Marc‐Elie , Théorêt Yves , Saliba Issam 
Published: May 2010

AbstractObjectives/Hypothesis:There is no approved agent to prevent cisplatin‐induced ototoxicity. Our objectives were to: 1) identify and compare the effect of intratympanic injections of lactate or N‐acetylcysteine (NAC) in the prevention of cisplatin‐induced ototoxicity, 2) investigate inner ear protection using a scanning electron microscope, and 3) study systemic diffusion of intratympanic NAC.Study Design:Prospective animal study.Methods:Sixteen guinea pigs formed two groups, and respectively received intratympanic lactate and 20% NAC in one ear. The contralateral ears received a control saline solution. After 30 minutes, an intraperitoneal cisplatin injection of 3 mg/kg was performed and repeated eight times, once a week, to achieve 24 mg/kg. Auditory brainstem responses (ABRs) were recorded before any injection, after 9 mg/kg and after 24 mg/kg of cisplatin for the frequencies 2, 4, 6 and 8 kHz. Cochleas were analyzed under scanning electron microscope. Systemic diffusion of NAC was studied using high‐performance liquid chromatography.Results:For the control ears, ABR thresholds increased uniformly and averaged 28.4 dB. The lactate group showed a lower threshold increase and averaged 17.0 dB. The NAC showed an important threshold increase of 89.0 dB. Lactate showed a significant hearing protection at 2000 Hz (P < .01). Electron microscopy revealed partial preservation of outer hair cell stereocilia for the ears treated with lactate and severe disruption for NAC group. No systemic diffusion of NAC was observed with chromatography.Conclusions:Intratympanic lactate offers significant partial protection against cisplatin‐induced ototoxicity at midfrequencies. High‐concentration NAC does not seem a viable solution as it causes a considerable inflammatory reaction. NAC does not diffuse systemically.
Barriers to the implementation of competency‐based education and assessment: A survey of otolaryngology program directors
Authors: Laeeq Kulsoom , Weatherly Robert A. , Masood Hamid , Thompson Richard E. , Brown David J. , Cummings Charles W. , Bhatti Nasir I. 
Published: May 2010

AbstractObjectives/Hypothesis:To identify the barriers faced by otolaryngology program directors as they implement competency‐based education and assessment and to identify preferred approaches to meet these challenges as suggested by program directors.Study Design:A national survey of otolaryngology–head and neck surgery program directors.Methods:We developed a 20‐item questionnaire that was distributed to 102 otolaryngology program directors through SurveyMonkey. Nonrespondents were reminded by follow‐up email and phone calls. Results were analyzed by descriptive statistical analysis.Results:A total of 88 (86%) program directors responded to the survey. There was a marked discrepancy between the income received and time spent performing the duties of the program director. Program director workload was recognized as the most important barrier to the implementation of competency‐based education. Creating a practical clearinghouse of existing and emerging assessment tools was given the highest rating among the approaches to meet the challenges faced by program directors.Conclusions:Program directors in otolaryngology do not have sufficient financial support, protected time, and personnel to fulfill their administrative and educational responsibilities. They should be provided with additional institutional assistance to help them achieve the goals of the Accreditation Council for Graduate Medical Education outcome project.
The etiology of otosclerosis: A combination of genes and environment
Authors: Schrauwen Isabelle , Van Camp Guy 
Published: May 2010

AbstractOtosclerosis is a common form of hearing loss characterized by abnormal bone remodeling in the otic capsule. It is a complex genetic disease, caused by a combination of genetic and environmental factors. During the past decade, several attempts have been made to identify factors for otosclerosis. This review provides an overview of the current understanding of the etiology of otosclerosis and describes the genetic and environmental factors that have been implicated in the disease. Environmental factors include fluoride and viral factors, particularly measles. Genetic association studies for otosclerosis have reported several associations of genetic variants that influence the risk of disease, mainly involving bone remodeling pathways, although their individual risk contributions are small. Rare monogenic forms of otosclerosis also exist, which are caused by a mutation in a single gene leading to a clear familial segregation of the disease. Linkage analysis of large otosclerosis families has led to the identification of seven loci, and recently evidence was found that T cell receptor beta is a gene responsible for familial otosclerosis, suggesting an underlying immunological pathway. However, this might also represent an autoimmune process, a hypothesis that is supported by other data as well. In conclusion, a variety of pathways have been identified to be involved in the development of otosclerosis, showing that distinct mechanisms involving both genetic and environmental risk factors can influence and contribute to a similar disease outcome.
Automorphisms of endomorphism semigroups of reflexive digraphs
Authors: Araüjo João , Dobson Edward , Konieczny Janusz 
Published: May 2010

AbstractA reflexive digraph is a pair (X, ρ), where X is an arbitrary set and ρ is a reflexive binary relation on X. Let End (X, ρ) be the semigroup of endomorphisms of (X, ρ). We determine the group of automorphisms of End (X, ρ) for: digraphs containing an edge not contained in a cycle, digraphs consisting of arbitrary unions of cycles such that cycles of length ≥2 are pairwise disjoint, and some circulant digraphs (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Group extensions and free actions by finite groups on solvmanifolds
Authors: Jo Jang Hyun , Lee Jong Bum 
Published: May 2010

AbstractThe purpose of this paper is to generalize Yalçin's result [Trans. Amer. Math. Soc. 352, 2689–2700 (2000)] from tori to solvmanifolds. As a result, we prove that if (ℤp)r acts freely on a solvmanifold M of dimension n, then r ≤ n (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Uniformly continuous superposition operators in the space of bounded variation functions
Authors: Matkowski Janusz 
Published: May 2010

AbstractLet I, J ⊂ ℝ be intervals. The main result says that if a superposition operator H generated by a function of two variables h: I × J → ℝ,H (φ)(x) ≔ h (x, φ (x)),maps the set BV (I, J) of all bounded variation functions, φ: I → J into the Banach space BV (I, ℝ) and is uniformly continuous with respect to the BV ‐norm, thenh (x, y) = a (x)y + b (x), x ∈ I, y ∈ J,for some a, b ∈ BV (I, ℝ) (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Members of the low‐density lipoprotein receptor‐related proteins provide a differential molecular signature between parental and CD133(+) DAOY medulloblastoma cells
Authors: Annabi Borhane , Doumit Jinane , Plouffe Karine , Laflamme Carl , Lord‐Dufour Simon , Béliveau Richard 
Published: May 2010

AbstractMembers of the low‐density lipoprotein receptor‐related protein (LRP) family are involved in metabolic stress and resistance phenotypes of cancer cells. New breakthroughs in brain cancer therapy have exploited that molecular signature and proved that efficient delivery of therapeutic agents involve LRP‐mediated mechanisms. We performed gene expression profiling of CD133, a cell surface cancer stem cell marker, and of LRP in response to in vitro nutrient deprivation. We found that CD133 was selectively induced in serum‐starved DAOY medulloblastoma cells but not in U87MG glioblastoma cells. Such CD133 induction was correlated to increases in LRP‐1 and LRP‐1b gene and protein expression. When a specific CD133(+) DAOY cell population was sorted from parental DAOY, we found increases in LRP‐5 and LRP‐8. Uptake of α2‐macroglobulin, a specific LRP‐1/1b ligand, was increased in serum‐starved parental DAOY cells but not in CD133(+) DAOY cells, and receptor‐associated protein (RAP), which binds to all cell surface LRPs, was able to compete for that uptake. Conversely, RAP binding was increased in serum‐starved parental DAOY but α2‐macroglobulin was unable to compete for such uptake. Strategies aiming at targeting cancer stem cell metabolic adaptative responses, such as that through LRP differential expression within the brain tissue microenvironmental niche, can now be envisioned. © 2010 Wiley‐Liss, Inc.
Synthesis and thermal behavior of a novel UV‐curable transparent flame retardant film and phosphorus‐nitrogen synergism of flame retardancy
Authors: Xing Weiyi , Song Lei , Jie Ganxin , Lv Xiaoqi , Wang Xin , Hu Yuan 
Published: May 2010

AbstractA novel phosphorus monomer (PDHA) has been synthesized through phenyl dichlorophosphate (PDPC) reacting with 2‐hydroxyethyl acrylate (HEA). The structure of PDHA was characterized by Fourier transform infrared spectroscopy (FTIR) and 1H nuclear magnetic resonance spectroscopy (1H NMR). A series of UV curable resins were manufactured by blending PDHA with triglycidyl isocyanurate acrylate (TGICA) at different weight ratios. The fire performance was examined by micro‐scale combustion calorimeter (MCC) and limiting oxygen index (LOI). The results obtained from MCC indicated that the addition of PDHA to TGICA reduced the HRR and HRC. In addition, the LOI values varied from 28 to 34. The char residues of the composites were observed by scanning electron microscopy (SEM). Their thermal degradation behavior was investigated by thermogravimetric analysis and real time FTIR analysis (RT‐FTIR). The test results indicated that when the weight ratio of PDHA/TGICA = 1:1, the onset temperature of the composite was highest and the most char residue at 700°C was observed. RT‐FTIR showed that the phosphate group of PDHA first degraded to form poly(phosphoric acid)s at around 300°C, which had the major contribution to form the compact char to protect the sample from further degradation. Copyright © 2010 John Wiley & Sons, Ltd.
Simultaneous determination of three naphthoquinones in the leaves ofImpatiens balsaminaL. by reversed‐phase high‐performance liquid chromatography
Authors: Sakunphueak Athip , Panichayupakaranant Pharkphoom 
Published: May 2010

AbstractIntroduction – Naphthoquinones; lawsone (1), lawsone methyl ether (2) and methylene‐3,3′‐bilawsone (3) are the main active compounds ofImpatiens balsaminaleaves.Objective – To develop and validate an HPLC method for simultaneous quantitative determination of1–3inI. balsaminaleaf extracts.Methodology – The method utilised a Supelco® C18column (5 µm, 4.6 × 150 mm) at 25°C with the mixture of 2% aqueous acetic acid : methanol (gradient elution as follows: 0–10 min, 25 : 75; 10–20 min, 32 : 68; 20–35 min, 55 : 45) as the mobile phase at a flow‐rate of 1 mL/min, and UV detection at 280 nm. The parameters of linearity, repeatability, reproducibility, accuracy specificity and sensitivity of the method were evaluated.Results – The recovery of the method was 96–101% and linearity (r2≥ 0.9995) was obtained for all naphthoquinones. A high degree of specificity, as well as repeatability and reproducibility (RSD less than 5%), were also achieved. Copyright © 2010 John Wiley & Sons, Ltd.
Implementing Data Standards: A report on the HUPOPSI Workshop September 2009, Toronto, Canada
Authors: Orchard Sandra , Albar Juan‐Pablo , Deutsch Eric W. , Eisenacher Martin , Binz Pierre‐Alain , Hermjakob Henning 
Published: May 2010

AbstractThe plenary session of the Proteomics Standards Initiative of the Human Proteome Organisation at the 8th Annual HUPO World Congress updated the delegates on the current status of the ongoing work of this group. The mass spectrometry group reviewed the progress of mzML since its release last year and detailed new work on providing a common format for SRM/MRM transition lists (TraML). The implementation of mzIdentML, for describing the output of proteomics search engines, was outlined and the release of a new web service PSICQUIC, which allows users to simultaneously search multiple interaction databases, was announced. Finally, the audience participated in a lively debate, discussing both the benefits of these standard formats and issues with their adoption and use in a research environment.
HUPO Biological Reference Material Initiative Workshop 26 September 2009, Toronto, Canada
Authors: Nagy Judit M. 
Published: May 2010

AbstractThe Biological Reference Material Initiative Workshop held at the Toronto HUPO congress on 26 September 2009, focused on the development of new biological reference materials and tools for the assessment of reproducibility, the solutions to many of the technical challenges in proteomics and protein‐based molecular diagnostics. This half‐day meeting included presentations from leading scientists from the worldwide proteomic community, who shared a common interest in standardization and increased accuracy of proteomic data. The conclusion was that proteomics is highly sensitive to both biological and technical variability. It is this biological and technical variance, when not accounted for by experiment design, that invalidates proteomic experiments, but both of these issues can be dealt with by tackling reproducibility.
Burning Behavior of Nitramine Gun Propellants under the Influence of Pressure Oscillations
Authors: Pauly Gert , Scheibel Rudolf 
Published: May 2010

AbstractBy means of DNDA nitramine fractions in the formulation, it is hoped to produce gun propellants that have an almost temperature‐independent burning behavior. The reason for this behavior is not clear yet. In the last years, it has become known that pressure oscillations may occur in the hole channels of gun propellant grains that may lead to a modification of the burning. To analyze the impact of such oscillations on the burning behavior of DNDA powders, tests with two different DNDA powders were performed in a closed vessel. In both cases, it could be demonstrated that the oscillations have a determining influence on the temperature behavior.
Defects in highly Mg‐doped AlN
Authors: Nonaka Kentaro , Asai Toshiaki , Nagamatsu Kentaro , Iwaya Motoaki , Kamiyama Satoshi , Amano Hiroshi , Akasaki Isamu 
Published: May 2010

AbstractMicrostructural analysis was conducted to observe Mg‐related defects in highly Mg‐doped AlN on an undoped AlN layer grown by metalorganic vapor phase epitaxy. Pyramidal defects at a density of 1015 cm−3 were observed in the Mg‐doped AlN layer with a Mg concentration of 2.5 × 1019 cm−3. The size and density of the pyramidal defects decreased at a low Mg‐doped AlN layer with a Mg concentration. When the Mg concentration is high, defects of different shapes were formed in the Mg‐doped AlN layer.
A screened hybrid density functional study on energetic complexes: Metal carbohydrazide nitrates
Authors: Huang Huisheng , Zhang Tonglai , Zhang Jianguo , Wang Liqiong 
Published: May 2010

AbstractThe molecular geometry, electronic structure and thermochemistry of a series of metal carbohydrazide nitrates were investigated using the Heyd–Scuseria–Ernzerhof (HSE) screened hybrid density functional. The results show that Ca, Sr, and Ba complexes have additional coordinated oxygen atoms from the nitrate ion, which differ obviously from Cu, Ni, Co, and Mg complexes in terms of the geometric structure. Detailed NBO analyses clearly indicate that the metal–ligand interactions in Cu, Ni, and Co complexes are covalent, whereas those of Mg, Ca, Sr, and Ba complexes are ionic in nature. Furthermore, the donor–acceptor interactions result in a reduction of occupancies of σCO and σNH orbitals. Consequently, the bond lengths increase and the bond orders decrease. Finally, the calculated heats of formation predict that the ionic alkaline‐earth metal carbohydrazide nitrates are more stable than the covalent transition metal carbohydrazide nitrates. It agrees well with the available experimental thermal stabilities, indicating that the metal–ligand bonding character plays an important role in the stabilities of these energetic complexes. © 2010 Wiley Periodicals, Inc. Int J Quantum Chem, 2011
A mechanism of the 1,3‐dipolar cycloaddition between the hydrogen nitryl HNO2 and acetylene HCCH: The electron localization function study on evolution of the chemical bonds
Authors: Berski Slawomir , Latajka Zdzislaw 
Published: May 2010

AbstractThe reaction between the simplest nitro compound HNO2 (hydrogen nitryl) and acetylene HCCH ‐ formally proceeding via 1,3‐dipolar cycloaddition ‐ has been studied by means of the B3LYP, MPW1K and MP2 methods. The energy barrier of 20.74 ÷ 32.91 kcal/mol is similar to ΔEa of the NNO + HCCH process but is essentially larger than computed for the reactions of HCCH with fulminic acid (HCNO) and NNCH2. Whole process is exothermic with the reaction energy: −10.87 ÷ −17.94 kcal/mol. An evolution of the chemical bonding has been analyzed by means of the Bonding Evolution Theory (BET) at the B3LYP/6‐31+G(d) and B3LYP/cc‐pVTZ levels. Two approximations of the reaction path have been considered, namely: the IRC and pseudo‐reaction paths. The reaction requires five steps and seven catastrophes of the fold and cusp type. A different effect of first fold catastrophe has been noticed. At the B3LYP/6‐31+G(d) level one of two nonbonding Vi=1,2(N) attractors is annihilated (F), meanwhile at B3LYP/cc‐pVTZ new V(N) attractor is created (F†). The chemical bonds are not formed/broken in TS. © 2010 Wiley Periodicals, Inc. Int J Quantum Chem, 2011
A global search algorithm of minima exploration for the investigation of low lying isomers of clusters from DFT‐based potential energy surface. A theoretical study of Sin and Sin − 1Al clusters
Authors: Marchal Rémi , Carbonnière Philippe , Pouchan Claude 
Published: May 2010

AbstractThe first and most important step in the study of properties and dynamics of atomic and molecular clusters is the determination of their microscopic structures. We have developed a new algorithm (Marchal et al., J Chem Phys 2009, 131, 114105), called global search algorithm of minima exploration, which includes two original schemes: the spheroidal generation, allowing the generation of rings, spheres, m‐rings cylinders and planar structures, and the raking optimization process allowing to discard step by step the conformations that become physically unrealistic during the optimization process. Using such an algorithm, the microscopic structure of the well‐known silicon clusters have been revisited as a test case as well as the doped Sin−1Al ones. © 2010 Wiley Periodicals, Inc. Int J Quantum Chem, 2010