bluuebunny/crossref_metadata_2025_split huggingface dataset:
Traumatic events and suicidal behaviour in the Nigerian Survey of Mental Health and Well‐Being
Authors: Uwakwe R. , Oladeji B. D. , Gureje O. 
Published: March 2012

Uwakwe R, Oladeji BD, Gureje O. Traumatic events and suicidal behaviour in the Nigerian Survey of Mental Health and Well‐Being.Objective:  Not much is known about the role of different traumatic events in predicting suicidal outcomes. We investigated the association of specific traumatic events with different suicidal outcomes.Method:  Data are from the Nigerian Survey of Mental Health and Well‐Being, a multistage probability household survey of persons aged 18 years and over. Information on traumatic events and suicidal behaviours (ideation, plan and attempts) was collected in face‐to‐face interviews using the Composite International Diagnostic Interview (CIDI.3) from a subsample of the respondents (N = 2143).Results:  At least one traumatic event was reported by 63% of the sample. Traumatic events were more likely to have been experienced by individuals with different suicidal outcomes, with a dose–response relationship between the number of traumatic events and suicide ideation. The risks of suicidal ideation were elevated among persons with a history of combat experience (OR 6.3 95% CI 1.8–21.8) and those with exposure to war (OR 4.2; 95% CI 1.6–10.6), while that of suicidal attempt was increased among persons with experience of interpersonal violence (OR 4.3; 95% CI 1.4–13.0).Conclusion:  Traumatic events are common in the general population. This report highlights the role of traumatic events (especially those related to violence) in predicting suicidal behaviour.
The influence of the HLA‐DRB1 and HLA‐DQB1 allele heterogeneity on disease risk and severity in Iranian patients with multiple sclerosis
Authors: Kollaee A. , Ghaffarpor M. , Ghlichnia H. A. , Ghaffari S. H. , Zamani M. 
Published: March 2012

SummaryMultiple sclerosis (MS) is a common autoimmune disorder of the central nervous system. Recent studies have shown that the HLA‐DRB1 and DQB1 alleles are associated with MS susceptibility and severity. However, this is controversial in different population studies. In the present study, the roles of HLA‐DRB1 and DQB1 alleles and the amino acids were investigated on disease risk and severity in 120 Iranian patients with MS and 120 controls. Our findings indicate that the DRB1*1501 allele (OR = 3.203 P = 0.001), the DRB1*1501‐DQB1*0602 haplotype (OR = 7.792 P = 0.003) and the DRB1*1501/0701‐ genotype (OR = 3.320 P = 0.006) and amino acid Leu26 (OR = 1.645 P = 0.005) and Phe9 (OR = 1.893 P = 0.009) on the DQβ1 chain are significantly associated with MS susceptibility. DRB1*1001 was the only allele that had a protective effect against MS (P = 0.0004). We also found that the DQB1*0303 allele was significantly associated with disease severity (mean Multiple Sclerosis Severity Score difference = 1.979, P = 0.002). However, protective effect of the DRB1*1001 against MS and also association of DQB1*0303 allele with MS severity need to be confirmed by larger sample size.
Australian Tropical and Subtropical Rain Forest Community Assembly: Phylogeny, Functional Biogeography, and Environmental Gradients
Authors: Kooyman Robert , Rossetto Maurizio , Allen Chris , Cornwell William 
Published: March 2012

AbstractTo compare community assemblage patterns in tropical northeastern and subtropical central eastern Australia across selected gradients and scales, we tested the relationship of species traits with phylogenetic structure, and niche breadth. We considered phylogenetic relationships across current‐day species in assemblages in relation to rain forest species pool sizes, and trait values along gradients including elevation and latitude. Trait values were quantified across scales for seed size, leaf area, wood density and maximum height at maturity for 1137 species and 596 assemblages using trait gradient analysis (TGA). Local assemblages of subtropical species had narrower trait ranges, and higher niche breadth values than corresponding assemblages of tropical species. Leaf size and seed size increased at low latitudes, and community phylogenetic structure was most strongly correlated with seed traits in the subtropics, reflecting dispersal and re‐colonization processes. Elevation accounted for little of the variance in community phylogenetic structure or trait variation across local and regional scales. Stable moist forest areas retained many species from ancestral rain forest lineages across a range of temporally conserved habitats; species within assemblages were less related; and rain forest assemblages had higher functional diversity, but lower niche breadth. This suggests that on average, assemblages of species in stable areas had greater trait variation and narrower distributions. Historic and recent rain forest contraction and re‐expansion can result in recolonized areas that are dominated by species that are more related (phylogenetically) than by chance, have smaller, widely dispersed seeds, and greater niche breadth (broader distributions).
Mortality caused by influenza and respiratory syncytial virus by age group in England and Wales 1999–2010
Authors: Hardelid P. , Pebody R. , Andrews N. 
Published: March 2012

Please cite this paper as: Hardelid et al. (2012) Mortality caused by influenza and respiratory syncytial virus by age group in England and Wales 1999–2010. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750‐2659.2012.00345.x.Background:  The mortality burden caused by influenza cannot be quantified directly from death certificates because of under‐recording; therefore, the estimated number of influenza deaths has to be obtained through statistical modelling.Objective:  To estimate the number of deaths caused by influenza and respiratory syncytial virus (RSV) in England and Wales between 1999 and 2010 using a multivariable regression model.Methods:  Generalised linear models were used to estimate weekly deaths by age group (<15, 15–44, 45–74 and 75+ years) as a function of positive influenza and RSV isolates. Adjustment was made for temperature variation (using weekly means of daily Central England temperature time series), underlying seasonal variation and temporal trends. The parameters from the model were used to predict the number of deaths caused by influenza and RSV across winter seasons.Results:  Between 7000 and 25 000 deaths across all ages were associated with influenza in the winter periods 1999–2009. The mortality burden was the highest among the over 75 age group, among whom 2·5–8·1% of deaths were caused by influenza. The lowest number of influenza deaths was estimated for the winter 2009/2010 when pandemic influenza A/H1N1 (2009) was the predominant circulating strain. RSV accounted for 5000–7500 deaths each winter season.Conclusions:  The model presented provides a robust and reasonable approach to estimating the number of deaths caused by influenza and RSV by age group at the end of each winter.
An assessment of prime‐boost vaccination schedules with AS03A‐adjuvanted prepandemic H5N1 vaccines: a randomized study in European adults
Authors: Gillard Paul , Caplanusi Adrian , Knuf Markus , Roman François , Walravens Karl , Moris Philippe , Dramé Mamadou , Schwarz Tino F. 
Published: March 2012

Please cite this paper as: Gillard et al. (2012) An assessment of prime‐boost vaccination schedules with AS03A‐adjuvanted prepandemic H5N1 vaccines: a randomized study in European adults. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750‐2659.2012.00349.x.Background  Long‐term persistence of immune response and safety of an H5N1 prepandemic influenza vaccine adjuvanted with AS03 (an α‐tocopherol oil‐in‐water emulsion‐based adjuvant system) was evaluated using various prime‐boost schedules that mimicked potential pandemic scenarios (NCT00430521).Methods  Five hundred and twelve healthy adults aged 18–60 years received primary vaccination with one or two doses (0, 21 days schedule) of the A/Vietnam/1194/2004 H5N1 vaccine followed by a booster dose (A/Vietnam/1194/2004 or A/Indonesia/05/2005 strain) six or twelve months later across eight randomized groups. Immunogenicity results by hemagglutination inhibition [HI] assay, microneutralization assay, and the cell‐mediated immune response (CMI) are reported here for the four groups boosted at Month 12.Results  A one‐dose‐adjuvanted primary administration followed 12 months later by a single‐adjuvanted booster dose containing a heterologous vaccine strain met or exceeded all US and European criteria for both strains. Increasing the interval between the first and second dose (from 21 days to 12 months) resulted in stronger cross‐reactive immune responses against the A/Indonesia/05/2005 strain. The HI antibody response against the two strains persisted for 6 months after the booster dose irrespective of the booster vaccine’s strain. The neutralizing antibody responses and the CMI observed in the study population paralleled the HI immune response. Overall, the vaccine had a clinically acceptable safety profile.Conclusion  The H5N1 vaccine in this study allowed for flexibility in the time interval between primary and booster vaccination and the use of a heterologous strain without impacting the strength of the humoral and cellular immune response to both vaccine strains.
Single‐dose inhaled laninamivir: registered in Japan and its potential role in control of influenza epidemics
Authors: Sunagawa Satoko , Higa Futoshi , Cash Haley L. , Tateyama Masao , Uno Tsukasa , Fujita Jiro 
Published: March 2012

Please cite this paper as: Sunagawa et al. (2012) Single‐dose inhaled laninamivir: registered in Japan and its potential role in control of influenza epidemics. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750‐2659.2012.00351.x.
Retinal vessel diameter can reliably be determined in minipigs using Retinal Vessel Analyser with a microscope‐mounted fundus camera
Authors: Papadopoulou Domniki N. , Mangioris George , Petropoulos Ioannis K. , Mendrinos Efstratios , Mavropoulos Anestis , Pournaras Constantin J. 
Published: March 2012

Abstract.Purpose:  Retinal Vessel Analyser (RVA) is a validated instrument to measure retinal vessel diameter in humans. The purpose of this study was to assess the reproducibility (inter‐observer reliability) and the repeatability (test–retest reliability) of RVA with a microscope‐mounted fundus camera to determine retinal vessel diameter in minipigs.Methods:  Ocular fundus image from five anaesthetized minipigs was recorded in a digital videotape for approximately 5 min, under stable systemic arterial pressure and gas conditions. To evaluate the reproducibility, each one of two investigators used RVA to measure the diameter of the superior temporal retinal artery on five separate 30‐second video sequences from each minipig, which were the same video sequences for both investigators. To evaluate the repeatability, one investigator performed five measurements on a single, randomly selected, 30‐second video sequence from each minipig. The reproducibility was determined using the intra‐class correlation coefficient (ICC), and the repeatability was assessed using the coefficient of variation (COV). Bland–Altman plots were also used to assess agreement between the two investigators.Results:  Retinal arteriolar diameter measurements with RVA in minipigs were highly reproducible. Differences between the two investigators were lower than 0.7%. The ICC was 1.00, indicating perfect reproducibility, and the mean COV was 0.18%, reflecting excellent repeatability of the measurements with RVA.Conclusion:  Retinal vessel diameter can reliably be determined not only in humans, but also in minipigs, using the commercially available RVA apparatus and a microscope‐mounted fundus camera.
Learning curve in the Cut and Paste method for surgery of primary pterygium
Authors: Koranyi Gabor , Artzén Ditte , Wijk Tomas 
Published: March 2012

Abstract.Purpose:  To evaluate the learning curve for the surgery of primary nasal pterygium with conjunctival autograft using fibrin adhesive (the Cut and Paste method).Methods:  The outcomes of the first consecutive 120 primary pterygium surgery cases for two surgeons (groups A and B) were investigated retrospectively and compared with the first (group C) and second (group D) 120 cases of GK. A and B were previously inexperienced in pterygium surgery. Outcome variables were surgery time, complications, the number and the temporal distribution of the recurrences. The statistics used were moving averages of the recurrences, Scheffé’s test of means, learning curve analysis according to Wright and logarithmic curve fitting to operation time data. The impact of age and sex on recurrences was tested using t‐tests and chi‐square tests.Results:  The mean surgery time was 17 min for A, 13 min for B and 13 min for GK. There was a certain learning curve slope in operation time in groups A and C. The postoperative complications were few and acceptable. There were ten (8.9%) recurrences in group A, eight (6.8%) in group B, six (5.5%) in group C and five (4.6%) in group D. The temporal distribution of the recurrences did not show any learning curve. Age and sex had no significant correlation with recurrence rate or surgery time (data not shown).Conclusions:  Our study indicates that the Cut and Paste technique is easy to learn and master. We did not find a significant learning curve regarding surgery time, recurrences or complications. In a clinical setting, it is beneficial to let a few, otherwise experienced eye surgeons learn the technique.
Genome Sequence of Mycoplasma hyorhinis Strain GDL-1
Authors: Calcutt Michael J. , Foecking Mark F. , Rosales Ruben S. , Ellis Richard J. , Nicholas Robin A. J. 
Published: March 2012

ABSTRACT

Mycoplasma hyorhinis
impacts swine health and production in many countries, either as a primary pathogen or as a component of a polymicrobial infection. Isolates of this species are also common contaminants of tissue culture lines. The genome sequence of the cell culture isolate
M. hyorhinis
GDL-1 is presented herein.
Draft Genome of Halomonas Species Strain GFAJ-1 (ATCC BAA-2256)
Authors: Phung Le T. , Silver Simon , Trimble William L. , Gilbert Jack A. 
Published: March 2012

ABSTRACTHalomonasstrain GFAJ-1 was reported inSciencemagazine to be a remarkable microbe for which there was “arsenate in macromolecules that normally contain phosphate, most notably nucleic acids.” The draft genome of the bacterium was determined (NCBI accession numbersAHBC01000001throughAHBC01000103). It appears to be a typical gamma proteobacterium.
Draft Genome Sequence of Streptomyces acidiscabies 84-104, an Emergent Plant Pathogen
Authors: Huguet-Tapia José C. , Loria Rosemary 
Published: March 2012

ABSTRACT

A draft genome sequence of the plant pathogen
Streptomyces acidiscabies
84-104, an emergent plant pathogen, is presented here. The genome is among the largest of streptomycetes, at more than 11 Mb, and encodes a 100-kb pathogenicity island (PAI) shared with other plant-pathogenic streptomycetes. The presence of this conserved PAI, and the remnants of a conserved integrase/recombinase at its 3′ end, supports the hypothesis that
S. acidiscabies
emerged as a plant pathogen as a result of this acquisition.
Physical exercise in southern Germany: a cross-sectional study of an urban population
Authors: Rupps Elli , Haenle Mark Martin , Steinacker Juergen , Mason Richard Andrew , Oeztuerk Suemeyra , Steiner Ronald , Kratzer Wolfgang ,   the EMIL Study group
Published: March 2012

ObjectivesThe aim of this study was to investigate the degree of physical exercise and self-assessment of physical fitness (PF) and their relationship to health- and behaviour-specific factors in a randomly selected sample of an urban population in southern Germany.DesignCross-sectional study.SettingIn the southern German city of Leutkirch.Participants2187 subjects randomly selected from the registry of inhabitants. Of the selected group, aged 18–65 years, 52.1% were women and 47.9% men.Primary and secondary outcome measuresParticipants were asked how many hours per week they spent on physical exercise and sports. They were also asked to rate their own performance and/or PF.ResultsOverall, 38.9% of the participants reported no physical exercise. Men reported a higher level of physical exercise than did women. Less exercise was reported by subjects with diabetes, high body mass index and waist-to-hip ratio and by those who were underweight. Alcohol consumption, smoker status and higher educational level showed a positive association with physical exercise. A negative trend with respect to moderate physical exercise was observed for those with metabolic syndrome, diabetes, hypertension and hepatic steatosis, but this was statistically significant only for subjects with diabetes. In both men and women, there was a relationship between self-assessed ‘good’ PF and high physical exercise.ConclusionsThe data show that a large proportion of the study population is not physically active; specific risk groups (overweight subjects, older subjects, smokers or subjects with low educational level) are even less active. The data suggest that there is a great potential for measures promoting physical exercise in these groups.
Circulating miR-15b and miR-130b in serum as potential markers for detecting hepatocellular carcinoma: a retrospective cohort study
Authors: Liu Angela M , Yao Tzy-Jyun , Wang Wei , Wong Kwong-Fai , Lee Nikki P , Fan Sheung Tat , Poon Ronnie T P , Gao Chunfang , Luk John M 
Published: March 2012

ObjectiveSerum α-fetoprotein (AFP) is the most commonly used biomarker for screening hepatocellular carcinoma (HCC) but fails to detect about half of the patients. Thus, we investigated if circulating microRNAs (miRNAs) could outperform AFP for HCC detection.DesignA retrospective cohort study.SettingTwo clinical centres in China.ParticipantsThe exploration phase included 96 patients with HCC who received primary curative hepatectomy, and the validation phase included 29 hepatitis B carriers, 57 patients with HCC and 30 healthy controls.Main outcome measuresExpression of miRNAs was measured by real-time quantitative reverse transcription–PCR. Areas under receiver operating characteristic curves were used to determine the feasibility of using serum miRNA concentration as a diagnostic marker for defining HCC. A multivariate logistic regression analysis was used to evaluate performances of combined serum miRNAs.ResultsIn the exploration phase, miRNA profiling on resected tumour/adjacent non-tumour tissues identified miR-15b, miR-21, miR-130b and miR-183 highly expressed in tumours. These miRNAs were also detectable in culture supernatants of HCC cell lines and in serum samples of patients. Remarkably, these serum miRNAs were markedly reduced after surgery, indicating the tumour-derived source of these circulating miRNAs. In a cross-centre validation study, combined miR-15b and miR-130b demonstrated as a classifier for HCC detection, yielding a receiver operating characteristic curve area of 0.98 (98.2% sensitivity and 91.5% specificity). The detection sensitivity of the classifier in a subgroup of HCCs with low AFP (<20 ng/ml) was 96.7%. The classifier also identified early-stage HCC cases that could not be detected by AFP.ConclusionThe combined miR-15b and miR-130b classifier is a serum biomarker with clinical value for HCC screening.
S-Glutathionylated Serine Proteinase Inhibitors as Plasma Biomarkers in Assessing Response to Redox-Modulating Drugs
Authors: Grek Christina L. , Townsend Danyelle M. , Uys Joachim D. , Manevich Yefim , Coker Woodrow J. , Pazoles Christopher J. , Tew Kenneth D. 
Published: March 2012

Abstract
Many cancer drugs impact cancer cell redox regulatory mechanisms and disrupt redox homeostasis. Pharmacodynamic biomarkers that measure therapeutic efficacy or toxicity could improve patient management. Using immunoblot analyses and mass spectrometry, we identified that serpins A1 and A3 were S-glutathionylated in a dose- and time-dependent manner following treatment of mice with drugs that alter reactive oxygen or nitrogen species. Tandem mass spectrometry analyses identified Cys256 of serpin A1 and Cys263 of serpin A3 as the S-glutathionylated residues. In human plasma from cancer patients, there were higher levels of unmodified serpin A1 and A3, but following treatments with redox active drugs, relative S-glutathionylation of these serpins was higher in plasma from normal individuals. There is potential for S-glutathionylated serpins A1 and A3 to act as pharmacodynamic biomarkers for evaluation of patient response to drugs that target redox pathways. Cancer Res; 72(9); 2383–93. ©2012 AACR.
Squamous Cell Carcinoma of the Lung: Molecular Subtypes and Therapeutic Opportunities
Authors: Perez-Moreno Pablo , Brambilla Elisabeth , Thomas Roman , Soria Jean-Charles 
Published: March 2012

Abstract
Lung cancer is the leading cause of cancer-related deaths worldwide. Next to adenocarcinoma, squamous cell carcinoma (SCC) of the lung is the most frequent histologic subtype in non–small cell lung cancer. Encouraging new treatments (i.e., bevacizumab, EGFR tyrosine kinase inhibitors, and ALK inhibitors) have afforded benefits to patients with adenocarcinoma, but unfortunately the same is not true for SCC. However, many genomic abnormalities are present in SCC, and there is growing evidence of their biologic significance. Thus, in the short term, the molecular characterization of patients with SCC in modern profiling platforms will probably be as important as deciphering the molecular genetics of adenocarcinoma. Patients with SCC of the lung harboring specific molecular defects that are actionable (e.g., fibroblast growth factor receptor 1 amplification, discoidin domain receptor 2 mutation, and phosphoinositide 3-kinase amplification) should be enrolled in prospective clinical trials targeting such molecular defects. Clin Cancer Res; 18(9); 2443–51. ©2012 AACR.
Dual mTORC1/2 and HER2 Blockade Results in Antitumor Activity in Preclinical Models of Breast Cancer Resistant to Anti-HER2 Therapy
Authors: García-García Celina , Ibrahim Yasir H. , Serra Violeta , Calvo Maria Teresa , Guzmán Marta , Grueso Judit , Aura Claudia , Pérez José , Jessen Katti , Liu Yi , Rommel Christian , Tabernero Josep , Baselga José , Scaltriti Maurizio 
Published: March 2012

Abstract
Purpose: The PI3K/Akt/mTOR pathway is an attractive target in HER2-positive breast cancer that is refractory to anti-HER2 therapy. The hypothesis is that the suppression of this pathway results in sensitization to anti-HER2 agents. However, this combinatorial strategy has not been comprehensively tested in models of trastuzumab and lapatinib resistance.
Experimental Design: We analyzed in vitro cell viability and induction of apoptosis in five different cell lines resistant to trastuzumab and lapatinib. Inhibition of HER2/HER3 phosphorylation, PI3K/Akt/mTOR, and extracellular signal-regulated kinase (ERK) signaling pathways was evaluated by Western blotting. Tumor growth inhibition after treatment with lapatinib, INK-128, or the combination of both agents was evaluated in three different animal models: two cell-based xenograft models refractory to both trastuzumab and lapatinib and a xenograft derived from a patient who relapsed on trastuzumab-based therapy.
Results: The addition of lapatinib to INK-128 prevented both HER2 and HER3 phosphorylation induced by INK-128, resulting in inhibition of both PI3K/Akt/mTOR and ERK pathways. This dual blockade produced synergistic induction of cell death in five different HER2-positive cell lines resistant to trastuzumab and lapatinib. In vivo, both cell line–based and patient-derived xenografts showed exquisite sensitivity to the antitumor activity of the combination of lapatinib and INK-128, which resulted in durable tumor shrinkage and exhibited no signs of toxicity in these models.
Conclusions: The simultaneous blockade of both PI3K/Akt/mTOR and ERK pathways obtained by combining lapatinib with INK-128 acts synergistically in inducing cell death and tumor regression in breast cancer models refractory to anti-HER2 therapy. Clin Cancer Res; 18(9); 2603–12. ©2012 AACR.