bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modulatory effects of phytoglycoprotein (75 kDa) on allergic inflammatory cytokines in Di(2‐ethylhexyl) phthalate (DEHP)‐stimulated RBL‐2H3 cells
Authors: Oh Phil‐Sun , Lim Kye‐Taek 
Published: November 2009

AbstractThis study investigated the inhibitory effect of a glycoprotein isolated fromCudrania tricuspidataBureau (CTB glycoprotein) on di(2‐ethylhexyl) phthalate (DEHP)‐induced mast cell degranulation and related signaling cascade in RBL‐2H3 cells. This experiment evaluated the intracellular Ca2+level, and the activities of protein kinase C (PKC), mitogen‐activated protein kinase (MAPK), transcription factor, and the cytokines in DEHP‐treated RBL‐2H3 cells. Our results revealed that the CTB glycoprotein in the presence of DEHP inhibits the release of histamine and expression of interleukin (IL)‐4, IL‐6, and TNF‐α in RBL‐2H3 cells. We also found that the CTB glycoprotein inhibits the intracellular Ca2+level, translocation of PKC from cytosol to membrane and the phosphorylation of ERK1/2 in cells. Moreover, the CTB glycoprotein (100 µg/ml) has suppressive effects on transcriptional activation of nuclear factor (NF)‐κB in DEHP‐treated RBL‐2H3 cells. The activation of NF‐κB was collectively blocked by treatment with PKC inhibitor (staurosporine) as well as ERK1/2 inhibitor (PD98059), respectively. The results from these experiments indicated that the CTB glycoprotein inhibits release of histamine and expressions of IL‐4, IL‐6, and TNF‐α via down regulations of PKC/MAPK and NF‐κB on the stage of mast cell degranulation induced by DEHP. Moreover, oral administration of CTB glycoprotein (10–20 mg/kg) inhibited compound 48/80‐mediated systemic reaction in mice. In conclusion, we speculated that the CTB glycoprotein might be one component for preparation of health supplements for prevention of allergic immune disorders. J. Cell. Biochem. 109: 124–131, 2010. © 2009 Wiley‐Liss, Inc.
Exploring hierarchical refinement techniques for induced fit docking with protein and ligand flexibility
Authors: Borrelli Kenneth W. , Cossins Benjamin , Guallar Victor 
Published: November 2009

AbstractWe present a series of molecular‐mechanics‐based protein refinement methods, including two novel ones, applied as part of an induced fit docking procedure. The methods used include minimization; protein and ligand sidechain prediction; a hierarchical ligand placement procedure similar to a‐priori protein loop predictions; and a minimized Monte Carlo approach using normal mode analysis as a move step. The results clearly indicate the importance of a proper opening of the active site backbone, which might not be accomplished when the ligand degrees of freedom are prioritized. The most accurate method consisted of the minimized Monte Carlo procedure designed to open the active site followed by a hierarchical optimization of the sidechain packing around a mobile flexible ligand. The methods have been used on a series of 88 protein‐ligand complexes including both cross‐docking and apo‐docking members resulting in complex conformations determined to within 2.0 Å heavy‐atom RMSD in 75% of cases where the protein backbone rearrangement upon binding is less than 1.0 Å α‐carbon RMSD. We also demonstrate that physics‐based all‐atom potentials can be more accurate than docking‐style potentials when complexes are sufficiently refined. © 2009 Wiley Periodicals, Inc. J Comput Chem, 2010
A scheme for rapid prediction of cooperativity in hydrogen bond chains of formamides, acetamides, andN‐methylformamides
Authors: Jiang Xiao‐Nan , Sun Chang‐Liang , Wang Chang‐Sheng 
Published: November 2009

AbstractA scheme is proposed in this article to predict the cooperativity in hydrogen bond chains of formamides, acetamides, andN‐methylformamides. The parameters needed in the scheme are derived from fitting to the hydrogen bonding energies of MP2/6‐31+G** with basis set superposition error (BSSE) correction of the hydrogen bond chains of formamides containing from two to eight monomeric units. The scheme is then used to calculate the individual hydrogen bonding energies in the chains of formamides containing 9 and 12 monomeric units, in the chains of acetamides containing from two to seven monomeric units, in the chains ofN‐methylformamides containing from two to seven monomeric units. The calculation results show that the cooperativity predicted by the scheme proposed in this paper is in good agreement with those obtained from MP2/6‐31+G** calculations by including the BSSE correction, demonstrating that the scheme proposed in this article is reasonable. Based on our scheme, a cooperativity effect of almost 240% of the dimer hydrogen bonding energy in long hydrogen bond formamide chains, a cooperativity effect of almost 190% of the dimer hydrogen bonding energy in long hydrogen bond acetamide chains, and a cooperativity effect of almost 210% of the dimer hydrogen bonding energy in long hydrogen bondN‐methylformamide chains are predicted. The scheme is further applied to some heterogeneous chains containing formamide, acetamide, andN‐methylformamide. The individual hydrogen bonding energies in these heterogeneous chains predicted by our scheme are also in good agreement with those obtained from Møller‐Plesset calculations including BSSE correction. © 2009 Wiley Periodicals, Inc. J Comput Chem, 2010
Functional multimerization of mucolipin channel proteins
Authors: Curcio‐Morelli Cyntia , Zhang Peng , Venugopal Bhuvarahamurthy , Charles Florie A. , Browning Marsha F. , Cantiello Horacio F. , Slaugenhaupt Susan A. 
Published: November 2009

AbstractMCOLN1 encodes mucolipin‐1 (TRPML1), a member of the transient receptor potential TRPML subfamily of channel proteins. Mutations in MCOLN1 cause mucolipidosis‐type IV (MLIV), a lysosomal storage disorder characterized by severe neurologic, ophthalmologic, and gastrointestinal abnormalities. Along with TRPML1, there are two other TRPML family members, mucolipin‐2 (TRPML2) and mucolipin‐3 (TRPML3). In this study, we used immunocytochemical analysis to determine that TRPML1, TRPML2, and TRPML3 co‐localize in cells. The multimerization of TRPML proteins was confirmed by co‐immunoprecipitation and Western blot analysis, which demonstrated that TRPML1 homo‐multimerizes as well as hetero‐multimerizes with TRPML2 and TRPML3. MLIV‐causing mutants of TRPML1 also interacted with wild‐type TRPML1. Lipid bilayer re‐constitution of in vitro translated TRPML2 and TRPML3 confirmed their cation channel properties with lower single channel conductance and higher partial permeability to anions as compared to TRPML1. We further analyzed the electrophysiological properties of single channel TRPML hetero‐multimers, which displayed functional differences when compared to individual TRPMLs. Our data shows for the first time that TRPMLs form distinct functional channel complexes. Homo‐ and hetero‐multimerization of TRPMLs may modulate channel function and biophysical properties, thereby increasing TRPML functional diversity. J. Cell. Physiol. 222: 328–335, 2010. © 2009 Wiley‐Liss, Inc.
1α,25‐dihydroxy vitamin D3 induces nuclear matrix association of the 1α,25‐dihydroxy vitamin D3 receptor in osteoblasts independently of its ability to bind DNA
Authors: Arriagada Gloria , Paredes Roberto , van Wijnen Andre J. , Lian Jane B. , van Zundert Brigitte , Stein Gary S. , Stein Janet L. , Montecino Martin 
Published: November 2009

Abstract1α,25‐dihydroxy vitamin D3 (vitamin D3) has an important role during osteoblast differentiation as it directly modulates the expression of key bone‐related genes. Vitamin D3 binds to the vitamin D3 receptor (VDR), a member of the superfamily of nuclear receptors, which in turn interacts with transcriptional activators to target this regulatory complex to specific sequence elements within gene promoters. Increasing evidence demonstrates that the architectural organization of the genome and regulatory proteins within the eukaryotic nucleus support gene expression in a physiological manner. Previous reports indicated that the VDR exhibits a punctate nuclear distribution that is significantly enhanced in cells grown in the presence of vitamin D3. Here, we demonstrate that in osteoblastic cells, the VDR binds to the nuclear matrix in a vitamin D3‐dependent manner. This interaction of VDR with the nuclear matrix occurs rapidly after vitamin D3 addition and does not require a functional VDR DNA‐binding domain. Importantly, nuclear matrix‐bound VDR colocalizes with its transcriptional coactivator DRIP205/TRAP220/MED1 which is also matrix bound. Together these results indicate that after ligand stimulation the VDR rapidly enters the nucleus and associates with the nuclear matrix preceding vitamin D3‐transcriptional upregulation. J. Cell. Physiol. 222: 336–346, 2010. © 2009 Wiley‐Liss, Inc.
Integrin‐linked kinase regulates migration and proliferation of human intestinal cells under a fibronectin‐dependent mechanism
Authors: Gagné David , Groulx Jean‐François , Benoit Yannick D. , Basora Nuria , Herring Elizabeth , Vachon Pierre H. , Beaulieu Jean‐François 
Published: November 2009

AbstractIntegrin‐linked kinase (ILK) plays a role in integrin signaling‐mediated extracellular matrix (ECM)–cell interactions and also acts as a scaffold protein in functional focal adhesion points. In the present study, we investigated the expression and roles of ILK in human intestinal epithelial cells (IECs) in vivo and in vitro. Herein, we report that ILK and its scaffold‐function interacting partners, PINCH‐1, α‐parvin, and β‐parvin, are expressed according to a decreasing gradient from the bottom of the crypt (proliferative/undifferentiated) compartment to the tip of the villus (non‐proliferative/differentiated) compartment, closely following the expression pattern of the ECM/basement membrane component fibronectin. The siRNA knockdown of ILK in human IECs caused a loss of PINCH‐1, α‐parvin, and β‐parvin expression, along with a significant decrease in cell proliferation via a loss of cyclin D1 and an increase in p27 and hypophosphorylated pRb expression levels. ILK knockdown severely affected cell spreading, migration, and restitution abilities, which were shown to be directly related to a decrease in fibronectin deposition. All ILK knockdown‐induced defects were rescued with exogenously deposited fibronectin. Altogether, our results indicate that ILK performs crucial roles in the control of human intestinal cell and crypt–villus axis homeostasis—especially with regard to basement membrane fibronectin deposition—as well as cell proliferation, spreading, and migration. J. Cell. Physiol. 222: 387–400, 2010. © 2009 Wiley‐Liss, Inc.
Spermatogenesis of the spider crabMaja brachydactyla(Decapoda: Brachyura)
Authors: Simeó Carles G. , Kurtz Kathryn , Chiva Manel , Ribes Enric , Rotllant Guiomar 
Published: November 2009

AbstractThis study describes spermatogenesis in a majid crab (Maja brachydactyla) using electron microscopy and reports the origin of the different organelles present in the spermatozoa. Spermatogenesis inM. brachydactylafollows the general pattern observed in other brachyuran species but with several peculiarities. Annulate lamellae have been reported in brachyuran spermatogenesis during the diplotene stage of first spermatocytes, the early and mid‐spermatids. Unlike previous observations, a Golgi complex has been found in mid‐spermatids and is involved in the development of the acrosome. The Golgi complex produces two types of vesicles: light vesicles and electron‐dense vesicles. The light vesicles merge into the cytoplasm, giving rise to the proacrosomal vesicle. The electron‐dense vesicles are implicated in the formation of an electron‐dense granule, which later merges with the proacrosomal vesicle. In the late spermatid, the endoplasmic reticulum and the Golgi complex degenerate and form the structures–organelles complex found in the spermatozoa. At the end of spermatogenesis, the materials in the proacrosomal vesicle aggregate in a two‐step process, forming the characteristic concentric three‐layered structure of the spermatozoon acrosome. The newly formed spermatozoa from testis show the typical brachyuran morphology. J. Morphol., 2010. © 2009 Wiley‐Liss, Inc.
Sperm ultrastructure of the spider crabMaja brachydactyla(Decapoda: Brachyura)
Authors: Simeó Carles G. , Kurtz Kathryn , Rotllant Guiomar , Chiva, Manel , Ribes Enric 
Published: November 2009

AbstractThis study describes the morphology of the sperm cell ofMaja brachydactyla, with emphasis on localizing actin and tubulin. The spermatozoon ofM. brachydactylais similar in appearance and organization to other brachyuran spermatozoa. The spermatozoon is a globular cell composed of a central acrosome, which is surrounded by a thin layer of cytoplasm and a cup‐shaped nucleus with four radiating lateral arms. The acrosome is a subspheroidal vesicle composed of three concentric zones surrounded by a capsule. The acrosome is apically covered by an operculum. The perforatorium penetrates the center of the acrosome and has granular material partially composed of actin. The cytoplasm contains one centriole in the subacrosomal region. A cytoplasmic ring encircles the acrosome in the subapical region of the cell and contains the structures‐organelles complex (SO‐complex), which is composed of a membrane system, mitochondria with few cristae, and microtubules. In the nucleus, slightly condensed chromatin extends along the lateral arms, in which no microtubules have been observed. Chromatin fibers aggregate in certain areas and are often associated with the SO‐complex. During the acrosomal reaction, the acrosome could provide support for the penetration of the sperm nucleus, the SO‐complex could serve as an anchor point for chromatin, and the lateral arms could play an important role triggering the acrosomal reaction, while slightly decondensed chromatin may be necessary for the deformation of the nucleus. J. Morphol., 2010. © 2009 Wiley‐Liss, Inc.
Trace LC/MS/MS quantitation of 17β‐estradiol as a biomarker for selective estrogen receptor modulator activity in the rat brain
Authors: Petucci Chris , Lloyd Tom , Harris Heather A. , Zhang Xiaochun , Chennathukuzhi Vargheese M. , Mekonnen Belew , Cai Yanxuan 
Published: November 2009

AbstractA sensitive LC/MS/MS method has been developed by derivatization of 17β‐estradiol (E2) with dansyl chloride to quantitate 17β‐E2 in female rat serum. The use of E2‐d5 minimized interferences from endogenous 17β‐E2 in order to achieve a limit of quantitation (LOQ) of 2.5 pg/ml using 150 µl of female rat serum. The recovery of the dansyl derivative was 95% or greater in quality control samples. The intra and interday assay precision was better than 8.2 and 6.2%, respectively, with accuracies ranging from 97 to 101% in the quality control samples. The assay was used for the quantitation of serum E2 as a biomarker for the estrogen receptor (ER) antagonist activity of small molecule SERMs (selective estrogen receptor modulators) in the female rat brain. The study revealed that a statistically significant upregulation of serum 17β‐E2 occurred for rats dosed with SERMs that are known to penetrate the brain and disrupt the hypothalamic‐pituitary‐ovarian (HPO) axis. Variations in 17β‐E2 in ascending dose studies also correlated with the corresponding trends in CYP17a1 levels, an mRNA biomarker for ovarian hyperstimulation. This biomarker assay has provided a useful screen for medicinal chemistry optimization to produce SERMs that do not interfere with negative feedback of estrogens on the brain and for biological hypothesis testing. Copyright © 2009 John Wiley & Sons, Ltd.
Normobaric hyperoximia increases hypoxia‐induced cerebral injury: DTI study in rats
Authors: Bockhorst K.H. , Narayana P.A. , Dulin J. , Liu R. , Rea H.C. , Hahn K. , Wosik J. , Perez‐Polo J.R. 
Published: November 2009

AbstractPerinatal hypoxia affects normal neurological development and can lead to motor, behavioral and cognitive deficits. A common acute treatment for perinatal hypoxia is oxygen resuscitation (hyperoximia), a controversial treatment. Magnetic resonance imaging (MRI), including diffusion tensor imaging (DTI), was performed in a P7 rat model of perinatal hypoxia to determine the effect of hyperoximia. These studies were performed on two groups of animals: 1) animals which were subjected to ischemia followed by hypoxia (HI), and 2) HI followed by hyperoximic treatment (HHI). Lesion volumes on high resolution MRI and DTI derived measures, fractional anisotropy (FA), mean diffusivity (MD), and axial and radial diffusivities (λl and λt, respectively) were measured in vivo one day, one week, and three weeks after injury. Most significant differences in the MRI and DTI measures were found at three weeks after injury. Specifically, three weeks after HHI injury resulted in significantly larger hyperintense lesion volumes (95.26 ± 50.42 mm3) compared to HI (22.25 ± 17.62 mm3). The radial diffusivity λt of the genu of corpus callosum was significantly larger in HHI (681 ± 330 × 10−6 mm2/sec) than in HI (486 ± 96 × 10−6 mm2/sec). Over all, most significant differences in all the DTI metrics (FA, MD, λt, λl) at all time points were found in the corpus callosum. Our results suggest that treatment of perinatal hypoxia with normobaric oxygen does not ameliorate, but exacerbates damage. © 2009 Wiley‐Liss, Inc.
Correlations between peripheral blood mononuclear cell production of BDNF, TNF‐alpha, IL‐6, IL‐10 and cognitive performances in multiple sclerosis patients
Authors: Patanella A.K. , Zinno M. , Quaranta D. , Nociti V. , Frisullo G. , Gainotti G. , Tonali P.A. , Batocchi A.P. , Marra C. 
Published: November 2009

AbstractThe aim of this study was to investigate the role of Brain Derived Neurotrophic Factor (BDNF) and inflammatory factors in the development of cognitive dysfunctions in Multiple Sclerosis (MS). We correlated peripheral blood mononuclear cell (PBMC) production of BDNF, Tumor Necrosis Factor‐alpha (TNF‐α), Interleukin (IL)‐6 and IL‐10 with performances on specific neuropsychological tasks in a selected series of MS patients. We studied a sample of 30 patients with relapsing‐remitting (RR)MS, segregated by gender and matched for age, education, disease duration, type of immunomodulating therapy, degree of disability and overall cognitive status. We found that low BDNF levels were correlated with increased time of execution on a divided attention and visual scanning task whereas high levels of IL‐6 were correlated with low Mini Mental State Examination scores. We did not observe any significant correlations between IL‐10, TNF‐α levels and cognitive performances in our patients. In conclusion our study shows a correlation between low BDNF and high IL‐6 production by PBMCs and poorer performances in cognitive tasks in RRMS patients suggesting a possible role of these factors in cognitive impairment in MS. © 2009 Wiley‐Liss, Inc.
The galanin receptor 2/3 agonist Gal2‐11 protects the SN56 cells against β‐amyloid25–35 toxicity
Authors: Pirondi S. , Giuliani A. , Del Vecchio G. , Giardino L. , Hökfelt T. , Calzà L. 
Published: November 2009

AbstractThe neuropeptide galanin is a modulator of cholinergic function and may play a role in Aβ peptide‐induced degeneration of cholinergic forebrain neurons. We have studied the effect of galanin and its galanin receptor subtype 2/3 agonist Gal2‐11on toxicity induced by freshly‐prepared β‐amyloid25–35 in the cholinergic cell line SN56. Both nuclear fragmentation and caspase‐3 expression were analysed. β‐amyloid25–35‐exposure induced a significant increase in caspase‐3 mRNA expression after 30, 60, 90 or 150 min of β‐amyloid25–35 exposure. These effects were abolished in the presence of Gal2‐11 (10 nM). Similarly, β‐amyloid25–35‐induced nuclear fragmentation was prevented by the galanin agonist at all time points studied. These findings indicate that the galanin 2/3 agonist Gal2‐11 protects SN56 cholinergic cells from β‐amyloid25–35‐induced cell death and that this action is mediated by an early reduction of caspase‐3 expression. © 2009 Wiley‐Liss, Inc.
Witnessed community and parental violence in relation to substance use and delinquency in a national sample of adolescents
Authors: Zinzow Heidi M. , Ruggiero Kenneth J. , Hanson Rochelle F. , Smith Daniel W. , Saunders Benjamin E. , Kilpatrick Dean G. 
Published: November 2009

AbstractThis study examined whether witnessed community and parental violence represented risk factors for substance use and delinquency among adolescents, beyond the contribution of direct violence and other risk factors. We also examined the role of violence characteristics. Participants were a national sample of 3,614 adolescents. Structured telephone interviews assessed demographics, trauma history, witnessed violence, delinquency, and substance use. While accounting for trauma history and other risk factors, witnessed community and parental violence were associated with delinquency. Community violence was associated with substance use. Chronic violence, knowing the perpetrator, and violence outside of school were correlated with substance use and delinquency among adolescents who witnessed community violence. These findings highlight the importance of targeting witnessed violence in prevention and intervention efforts.
Consumers' inferences about quality across diverse service providers
Authors: Reimer Anja , Folkes Valerie 
Published: November 2009

AbstractWhen consumers encounter different services as part of the same service experience they may use information about one group of service providers to draw inferences about the quality they expect to receive from another group of service providers. This research identifies the conditions under which information about the quality of one type of service (e.g., a hotel's front desk) influences quality perceptions of another type of service (e.g., a hotel's tour services). Results show that consumers' beliefs about managerial control influence generalizations across service providers. Also, consumers infer managerial control from the spatial proximity between the premises of the service providers. © 2009 Wiley Periodicals, Inc.
Effects of mushroom‐derived β‐glucan‐rich polysaccharide extracts on nitric oxide production by bone marrow‐derived macrophages and nuclear factor‐κB transactivation in Caco‐2 reporter cells: Can effects be explained by structure?
Authors: Volman Julia J. , Helsper Johannes P. F. G. , Wei Song , Baars Johan J. P. , van Griensven Leo J. L. D. , Sonnenberg Anton S. M. , Mensink Ronald P. , Plat Jogchum 
Published: November 2009

AbstractMushrooms are known for their immune‐modulating and anti‐tumour properties. The polysaccharide fraction, mainly β‐glucans, is responsible for the immune‐modulating effects. Fungal β‐glucans have been shown to activate leukocytes, which depend on structural characteristics of β‐glucans. As edible mushrooms come in contact with the intestinal immune system, effects on enterocytes are also interesting. Our aim was to evaluate the effect of mushroom polysaccharide extracts varying in β‐glucan structure on nitric oxide production by bone marrow‐derived macrophages (BMMs) from mice and on nuclear factor‐κB transactivation in human intestinal Caco‐2 cells. We demonstrated that extracts from Agaricus bisporus stimulated nitric oxide production by BMM, whereas extracts from Coprinus comatus and spores of Ganoderma lucidum had only minor effects. Furthermore, extracts of A. blazei Murill and Phellinus linteus had no effect at all. Almost all mushroom extracts lowered nuclear factor‐κB transactivation in Caco‐2 cells. Structural analysis of A. bisporus compared with A. blazei Murill suggests that branching of the β‐glucan chain is essential for immune‐stimulating activity. In conclusion, extracts from A. bisporus activate BMM, without activating enterocytes. These characteristics make A. bisporus an attractive candidate as a nutritional compound to stimulate the immune response in depressed states of immunity.