bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relationship Between the Words of Prayer, Psychological Well‐being, and Sense of Coherence
Authors: Fukuromoto Kumiko , Abe Shingo 
Published: February 2025

AbstractScientific studies on prayer provide little empirical evidence on the relationship between prayer and well‐being. Furthermore, although prayer was shown to be related to a sense of coherence, an ability concept for stress coping and health maintenance, studies have not examined the type of prayer linked to this association. Our study focused on words of prayer and clarified their association with psychological well‐being and sense of coherence. A questionnaire survey was conducted with 294 participants. No significant correlation was observed between words used in prayers and sense of coherence. However, specific words of prayer had different effects on psychological well‐being. Prayers of thanksgiving and vows consistently yielded positive outcomes regarding psychological well‐being and frequency and internal aspects of the prayer. Therefore, this study suggests that certain prayers may be more effective at improving well‐being than others. Nevertheless, in order to reach a definitive conclusion, it is necessary to focus on other aspects of prayer and to examine causal relationships in the future.
Targeting the Infrapatellar Branch of the Saphenous Nerve for Pain Relief in Patients With Acute or Chronic Knee Pain: A Systematic Review of Randomized Controlled Trials and Cohort Studies
Authors: Li Qiangqiang , Aierken Aikeremu , Qin Jianghui , Chen Dongyang , Jiang Qing 
Published: February 2025

ABSTRACTBlocking the infrapatellar branch of the saphenous nerve (IPBSN) can provide analgesic benefits for patients with postoperative acute pain or chronic pain, with minimal adverse effects. To evaluate the analgesic efficacy and potential adverse events associated with IPBSN block in patients suffering from acute or chronic knee pain. We conducted a systematic review across PubMed, Cochrane, Web of Science, and Embase to identify all relevant randomized controlled trials (RCTs) and cohort studies according to predefined selection criteria. The study quality of the RCTs was evaluated using the Cochrane risk of bias assessment tool, while cohort studies were assessed using the ROBINS‐I risk of bias tool. The primary outcomes measured were pain intensity and opioid consumption following the nerve block. A total of eight studies were included in this systematic review, encompassing 613 subjects with 276 participants in the control group and 337 participants in the IPBSN block group. The level of evidence was rated high for the RCTs and moderate for the cohort studies. The nerve block was administrated either through the injection of local anesthetic or percutaneous cryoneurolysis targeting the IPBSN. The results indicated that the IPBSN block significantly improved pain relief and reduced opioid consumption in patients with acute postoperative or chronic pain, with no significant difference in the rate of adverse events relating to the procedures or device. The IPBSN block holds promise for improving pain relief and reducing opioid consumption. However, further well‐designed randomized controlled trials are needed to confirm these results.
Upgrading of the genetic engineering toolkit accelerated the discovery process of the virulence effect of PsGH7d on Phytophthora sojae invasion
Authors: Liu Changqing , Tan Xinwei , Wang Jiayu , Sun Yujing , Xu Qian , Han Chao , Wang Qunqing 
Published: February 2025

AbstractThe genus of Phytophthora includes numerous phytopathogens that have devastating impacts on agricultural production. However, the limited availability of selection markers for numerous pathogenicity pathogens of the genus Phytophthora genetic transformation hinders further research on their pathogenic functional genes. Here we report a gene of NAT I, which serves as a novel selection marker for the Phytophthora sojae transformation. Additionally, we developed a new genetic manipulation toolkit based on vectors containing NAT I, which facilitates gene editing in P. sojae. With the toolkit, the gene PsGH7d of P. sojae, which encodes a glycosyl hydrolase, was edited consecutively via the CRISPR/Cas9 system to obtain gene knockout and enzymatic active site mutation strains. The pathogenicity analysis of these transformants revealed that PsGH7d is a virulence factor dependent on its bifunctional glucanase‐xylanase activities. This study develops an updated toolkit for the genus Phytophthora genetic transformation and provides initial insights into the virulence of the bifunctional enzyme PsGH7d.
Identification of Four Novel HLA Alleles: HLA‐C*03:09:02:01, ‐C*07:04:32, ‐C*07:1152 and ‐C*08:300
Authors: Fabreti‐Oliveira Raquel Aparecida , de Oliveira Cintia Keilla Fabreti , da Silva Assis Aline , Nascimento Evaldo 
Published: February 2025

ABSTRACTIdentification of four novel HLA alleles, HLA‐C*03:09:02:01, ‐C*07:04:32, ‐C*07:1152, and ‐C*08:300, in Brazilian individuals.
A Cautionary Tale: ABO Genotyping in Kidney Transplantation of ABO A2 Kidneys Into B or O Recipients
Authors: Murphey Cathi L. , Bailey Amanda , Kapturczak Matthias , Gebel Howard M. , Bray Robert A. 
Published: February 2025

ABSTRACTThe US 2014 kidney allocation system (KAS) allows ABO A2 donor kidneys to be transplanted into ABO B candidates, thereby broadening the donor pool for a traditionally disadvantaged population. Safely transplanting these patients relies heavily on two components: (1) recipient ABO antibody titres and (2) ABO A subtyping of the donor. While lectin‐based serological methods remain the gold standard for ABO typing, recent data show ABO molecular genotyping is a more reliable approach to accurately assign the A2 subtype. ABO titres were performed using Ortho Diagnostics gel cards using haemagglutination by both antiglobulin‐enhanced and dithiothreitol methods. ABO genotyping was performed using innotrain diagnostiks Real‐Time PCR. Here, we present two cases where ABO lectin testing incorrectly identified the donors as ABO A2 and A2B instead of A1 and A1B. Consequently, the transplants proceeded but had deleterious outcomes. These two cases support the concept that ABO genotyping by molecular methodology should be mandatory to confirm the A2 donor subtype when transplanting kidneys from deceased donors into ABO B candidates on the waitlist and for virtual crossmatching (VXM) donors being considered for transplantation into ABO B and O recipients.
Anthocyanin biosynthesis and transport synergistically modulated by RcMYB75 and RcGSTFL11 play a pivotal role in the feedforward loop in response to drought stress
Authors: Ma Mengni , Li Runhui , Li Yajun , Dai Wenhao , Shang Junzhong , He Yanhong , Xiang Fayun , Yang Yuanyuan , Wang Jihua , Huang Zifeng , Luo Hong , Zhang Jie , Ning Guogui 
Published: February 2025

SUMMARYAnthocyanins, the important antioxidants and signaling molecules, are natural polyphenolic compounds widely present in plants and essential for plant defense. However, little is known about the mechanisms underlying plant anthocyanin accumulation in relation to drought stress. This study reveals that drought stress induces significant anthocyanin accumulation in Rosa chinensis, alongside an increase in the expression of the MYB transcription factor (TF) gene RcMYB75 and the glutathione S‐transferase (GST) gene RcGSTFL11. When overexpressed, RcMYB75 markedly increases anthocyanin contents in both roses and tobaccos; conversely, reducing its expression significantly lowers anthocyanin contents in rose petals. RcGSTFL11 was confirmed as an anthocyanin transporter and overexpression of RcGSTFL11 can restore the anthocyanin‐deficient phenotype in the Arabidopsis tt19 mutant. Transgenic roses overexpressing RcGSTFL11 exhibit enhanced anthocyanin accumulation, while those with downregulated RcGSTFL11 have reduced contents. Transcriptomic analysis indicates that RcMYB75 upregulates the expression of key genes in the anthocyanin biosynthetic pathway and the anthocyanin transport gene RcGSTFL11. Ultimately, we also found that anthocyanin accumulation in these transgenics further enhances plant resistance to drought stress. Taken together, RcMYB75 and RcGSTFL11 promote the synthesis and transport of anthocyanins and play a key role in the feedforward loop responding to drought stress in roses. This study provides insights into the molecular mechanisms by which MYB TFs contribute to anthocyanin biosynthesis and transport, as well as the adaptive strategies of roses in response to drought stress.
Genetic dissection of internode length confers improvement for ideal plant architecture in maize
Authors: Duan Haiyang , Li Jianxin , Xue Zhengjie , Yang Lu , Sun Yan , Ju Xiaolong , Zhang Jihong , Xu Guoqiang , Xiong Xuehang , Sun Li , Xu Shuhao , Xie Huiling , Ding Dong , Zhang Xuebin , Zhang Xuehai , Tang Jihua 
Published: February 2025

SUMMARYThe optimal plant architecture, characterized by short stature, helps mitigate lodging, enables high‐density planting, and facilitates mechanized harvesting. Internode length (IL), a crucial component of plant height in maize, plays a significant role in these processes. However, the genetic mechanisms underlying internode elongation remain poorly understood. In this study, we conducted a genome‐wide association study to dissect the genetic architecture of IL in maize. The lengths of five internodes above and below the ear (referred as IL‐related traits) were collected across multiple environments, revealing substantial variation. A total of 108 quantitative trait loci (QTL) were associated with 11 IL‐related traits, with 17 QTL co‐detected by different traits. Notably, three QTL have been selected in maize breeding progress. Three hundred and three genes associated with IL were found to operate through plant hormone signal transduction, receptor activity, and carbon metabolism pathways, influencing internode elongation. ZmIL1, which encodes alcohol dehydrogenase, exhibited a high expression level in internodes during the vegetative stage and has been selected in Chinese modern maize breeding. Additionally, ZmIL2 and ZmIL3 emerged as other crucial regulators of IL. Importantly, ZmIL1 has potential applications in maize varieties in the Huang‐Huai‐Hai region. This study represents the first comprehensive report on the genetic architecture of nearly all ILs in maize, providing profound insights into internode elongation mechanisms and genetic resources. These findings hold significant implications for dwarf breeding programs aimed at optimizing plant architecture for enhancing agronomic performance.
The link between sarcopenic obesity and Alzheimer's disease: a brain‐derived neurotrophic factor point of view
Authors: Copeland Emily N. , LeBlanc Paul J. , Duarte‐Guterman Paula , Fajardo Val A. , MacPherson Rebecca E. K. 
Published: February 2025

AbstractAge‐related diseases are becoming more prominent as the lifespan of the global population rises. Many of these diseases coincide with each other and can even influence the onset of additional comorbidities. Sarcopenic obesity is described as age‐related loss of muscle mass that concurs with excessive weight gain and tends to increase the risk of comorbidity development, including Alzheimer's disease (AD). Though the exact link between sarcopenic obesity and AD is not known, this review explores the possibility that reduced levels of brain‐derived neurotrophic factor (BDNF) throughout the body may serve as the underlying commonality. In AD, reductions in BDNF signalling through its receptor promote the activation of glycogen synthase kinase 3 beta (GSK3β), which subsequently increases the production of amyloid beta (Aβ) peptides and neurofibrillary tangles (NFTs). In the skeletal muscle, lower BDNF concentrations are linked to impaired muscle fibre repair and regeneration, increasing the likelihood of sarcopenia. Furthermore, the absence of BDNF impairs mitochondrial function, leading to insulin resistance and increased adiposity. BDNF concentration has a negative relationship with obesogenic markers in adipose tissue, and as such, lower concentrations of BDNF lead to weight gain. Collectively, current literature suggests that BDNF attenuates AD pathology while improving skeletal muscle mitochondrial function, whole‐body insulin resistance and facilitating adipocyte browning. Therefore, BDNF may be a viable target for multiple age‐related diseases, but more research is required to substantiate this claim, with a particular focus on examining any potential influence of biological sex, as women are at a higher risk for both AD and sarcopenic obesity.
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Pattern Optimization to Minimize Dynamic Impacts of Wrapping Cables in Plate-like Space Structures: Experimental Validation
Authors: Oluyemi Momoiyioluwa , Agrawal Pranav , Salehian Armaghan 
Published: February 2025

Abstract
In lightweight space structures, incorporating power and control signal transmission cables significantly impacts the dynamics of the host structures. This study presents an experimental effort to provide proof of concept for the mathematical modeling techniques developed for the ideal cable placement to preserve the dynamic properties of host plate structures. By assuming a periodic cable wrapping pattern, the cable-harnessed structure is modeled using repeating fundamental elements. This periodicity enables the application of an energy-equivalence homogenization method, leading to partial differential equations that describe the out-of-plane vibrations of the cable-harnessed plate system. The presented test results further validate several optimal wrapping designs for host plate structures. The frequency response functions for the optimal cable pattern from the analytical model are compared against the test results, demonstrating excellent agreement.
Exploring the Development of Fundamental Movement Skills in Children With Physical Disabilities: A Scoping Review
Authors: Hebinck Margaux , Labbé Mariève , Lavoie Marie-Denise , Best Krista L. , Robert Maxime T. 
Published: February 2025

Introduction: Fundamental movement skills (FMS) are categorized into three groups (stability, locomotion, and object control) and are crucial for enabling the practice of physical activity in children with physical disabilities. Their development is influenced by the specific nature of each child’s disability. FMS can be evaluated by process- (movement execution) or product-oriented (movement outcome) assessments. This scoping review explored FMS development in children with physical disabilities and its relationship with physical activity. Method: Following Preferred Reporting Items for Systematic review and Meta-Analysis extension for scoping reviews guidelines, the search was conducted across six databases (MEDLINE, CINAHL, Web of Science, SPORTDiscus, EMBASE and PsycINFO) and used keywords related to FMS, physical literacy, and physical disabilities. Articles were included if participants had physical disabilities, were aged between 3 and 18 years, and if they explored the relationship between FMS and physical activity and/or physical literacy. Results: Twenty-two studies were included. Both process- and product-oriented assessments reported lower FMS proficiency in children with physical disabilities. Older children demonstrated better proficiency. Most studies highlighted a significant relationship between FMS and physical activity. Conclusion: Enhanced knowledge of effective strategies and evidence-based practices will enable practitioners to create supportive environments, encourage active participation, and track progress, ultimately leading to improved motor skills and overall well-being in children.
Microfluidic droplets with amended culture media cultivate a greater diversity of soil microorganisms
Authors: Dai Jing , Ouyang Yang , Gupte Rohit , Liu Xiao Jun A. , Li Yuwen , Yang Fang , Chen Shaorong , Provin Tony , Van Schaik Erin , Samuel James E. , Jayaraman Arul , Zhou Aifen , de Figueiredo Paul , Zhou Jizhong , Han Arum 
Published: February 2025

ABSTRACT

Uncultivated but abundant soil microorganisms have untapped potential for producing broad ranges of natural products, as well as for bioremediation. However, cultivating soil microorganisms while maintaining a broad microorganism diversity to enable phenotyping and functional analysis of as diverse individual isolates as possible remains challenging. In this study, we developed and tested the ability of several culture media formulations that contain defined soil metabolites or soil extracts to maintain microorganism diversity during culture. We also assessed their performance in microfluidic droplet cultivation where single-soil microorganism isolates were encapsulated and cultivated in picoliter-volume water-in-oil emulsion droplets to enable clonal growth needed for downstream functional analyses. Our results show that droplet cultivation with media supplemented by soil extract or soil metabolites enables the recovery of soil microorganisms with higher diversity (up to 1.5-fold higher richness) compared to bulk cultivation methods. Importantly, 1.7-fold more of less abundant (<1%) phyla and 11-fold more of unique genera were recovered, demonstrating the utility of this method for interrogating highly diverse soil microorganisms for broad ranges of applications.

IMPORTANCE

Although soil microorganisms hold a significant value in bioproduction and bioremediation, only a small fraction—less than 1%—can be cultured under specific media and cultivation conditions. This indicates that there are ample opportunities in harvesting the diverse environmental microorganisms if isolating and recovering these uncultured microorganisms are possible. This paper presents a new cultivation technique composed of isolating single-soil microorganism cell from an
in situ
soil microorganism community in microfluidic droplets and conducting in-droplet cultivation in media supplemented by soil extract or soil metabolites. This method enables the recovery of a broader diversity of the original microorganism community, laying the groundwork for a high-throughput phenotyping of these diverse microorganisms from their natural habitats.
Plus-strand RNA viruses hijack Musashi homolog 1 to shield viral RNA from cytoplasmic ribonuclease degradation
Authors: Zhou Defang , Xu Menglu , Liu Qingjie , Xin Ruixue , Cui Gege , Ding Longying , Liu Xiaoyang , Zhang Xinyue , Yan Tianxing , Zhou Jing , He Shuhai , Yang Liangyu , Xiang Bin , Cheng Ziqiang 
Published: February 2025

ABSTRACT

A successful strategy employed by RNA viruses to achieve replication is to evade host cell RNase degradation. However, the mechanisms through which plus-strand RNA viruses effectively shield viral RNA from cellular ribonuclease degradation remain unclear. In this study, we identified the phenomenon whereby plus-strand RNA viruses, including avian leukosis virus subgroup J (ALV-J), reticuloendotheliosis virus (REV), chicken astrovirus (CAstV), and porcine epidemic diarrhea virus (PEDV), hijacked host cellular Musashi homolog 1 (MSI1). These viruses upregulated MSI1 expression and facilitated its translocation from the cytoplasmic periphery to a position proximal to and within the nucleus, thereby protecting viral RNA from degradation. Mechanistic analyses revealed that these viruses use distinct regions, the unique (U3) region or three prime untranslated region (3′UTR), to engage with MSI1, consequently shielding their viral RNA from cytoplasmic ribonuclease degradation. These results offer significant implications for understanding the replication tactics used by plus-strand RNA viruses, thereby advancing our understanding of their biological behaviors.

IMPORTANCE
The intricate interplay between RNA viruses and host cell RNA regulation encompasses viral mechanisms designed to circumvent RNase-mediated degradation. However, the specific strategies employed by plus-strand RNA viruses to shield their RNA from host ribonucleases remain inadequately characterized. In this study, Musashi homolog 1 (MSI1) is predominantly localized in the cytoplasm of normal cells, distinct from the nucleus. Following infection by plus-strand RNA viruses such as avian leukosis virus subgroup J (ALV-J), reticuloendotheliosis virus (REV), chicken astrovirus (CAstV), and porcine epidemic diarrhea virus (PEDV), these viruses hijack MSI1 to relocate near and within the nucleus. This hijacking is facilitated by specific regions, including unique or three prime untranslated regions, thereby preventing viral RNA from degradation by cytoplasmic ribonucleases. These findings have significant implications for elucidating the replication strategies of plus-strand RNA viruses, thereby advancing our understanding of their biological mechanisms.
FBXO45 restricts HIV-1 replication by inducing SQSTM1/p62-mediated autophagic degradation of Tat
Authors: Xu Mingxiu , Hu Haobo , Yang Weijing , Zhang Jiaxiang , Wang Hong , Zhang Wenyan , Huan Chen 
Published: February 2025

ABSTRACT

As a key regulator of human immunodeficiency virus type 1 (HIV-1) transcription, Tat plays an essential role in viral replication and latency, making it a promising target for designing viral control strategies. Identifying host factors that modulate Tat and exploring the underlying mechanisms will benefit our understanding of HIV-1 transcriptional regulation and provide valuable insights into Tat-based therapeutic strategies. Here, by employing the TurboID approach, we discovered high-affinity binding between FBXO45 and Tat. Our findings demonstrate that FBXO45 negatively regulates Tat by promoting Tat ubiquitination and directing it to autophagic degradation. Autophagic degradation of Tat has been reported, but the specific underlying mechanisms remain unidentified. We elucidated this issue by providing evidence that FBXO45-mediated Tat polyubiquitination is an essential prerequisite for this process. Silencing of FBXO45 leads to a deficiency of autophagy receptor SQSTM1/p62 to bind and facilitate the autophagic degradation of Tat. Our results further underscore the crosstalk between post-translational modifications of Tat by demonstrating that the phosphorylation site of the Tat S62 residue is required for ubiquitination induced by FBXO45. Furthermore, in the context of the regulation of HIV-1, FBXO45 inhibits viral replication and maintains the latency of HIV-1 by suppressing viral transcription. Importantly, FBXO45 overexpression significantly attenuated viral rebound after antiretroviral therapy withdrawal. In summary, our findings suggest a novel role for FBXO45 in regulating HIV-1 replication by inducing the ubiquitination and SQSTM1/p62-dependent autophagic degradation of Tat. Considering the indispensable role of Tat in the regulation of HIV-1 replication and reactivation, FBXO45 may be a potential target for therapeutic intervention against HIV-1.

IMPORTANCE
HIV-1 Tat plays an indispensable role in regulating viral transcription and is a promising target for achieving a functional cure for AIDS. Identifying the host factors that modulate Tat expression could benefit the development of anti-HIV-1 strategies targeting Tat. Using TurboID assay, we identified a significant interaction between FBXO45 and Tat. Functionally, FBXO45 ubiquitinates and directs Tat for SQSTM1/p62-mediated autophagic degradation, thereby effectively restricting HIV-1 replication and maintaining HIV-1 latency by suppressing Tat-dependent viral transcription. These findings uncover a novel role for FBXO45 in regulating Tat and broaden our understanding of the host mechanisms involved in Tat processing.
Draft genomes of three
Sneathia vaginalis
isolates from a patient with bacterial vaginosis
Authors: Panzer Jonathan J. , McGinnis Natalie , Blom Jochen , Winters Andrew D. , Sobel Jack D. , Theis Kevin R. 
Published: February 2025

ABSTRACT

Sneathia vaginalis,
a fastidious pathogen of the female reproductive tract, is implicated in obstetric and gynecologic pathologies, including spontaneous preterm birth and bacterial vaginosis. Here, we report the successful cultivation and genomic sequencing of three
Sneathia vaginalis
isolates collected via a vaginal swab from a patient with bacterial vaginosis.