bluuebunny/crossref_metadata_2025_split huggingface dataset:
Heat shock protein 90α reduces CD8+ T cell exhaustion in acute lung injury induced by lipopolysaccharide
Authors: Yan Lei , Chen Yumei , Yang Yilin , Han Yi , Tong Chaoyang 
Published: June 2024

AbstractCD8+ T-cell exhaustion is a promising prognostic indicator of sepsis-induced acute respiratory distress syndrome (ARDS). Patients with sepsis-related ARDS had reduced levels of HSP90AA1. However, whether the changes in CD8+ T cells were related to HSP90α, encoded by the HSP90AA1 gene, was unclear. This study aimed to examine the regulatory mechanism of HSP90α and its impact on CD8+ T-cell exhaustion in lipopolysaccharide (LPS)-induced acute lung injury (ALI). In this study, by conducting a mouse model of ALI, we found that one week after LPS-induced ALI, CD8+ T cells showed exhaustion characteristics. At this time, proliferation and cytokine release in CD8+ T cells were reduced. The inhibitory costimulatory factors PD-1 and Tim-3, on the other hand, were enhanced. Meanwhile, the expression of HSP90α and STAT1 decreased significantly. The in vitro studies showed that HSP90α stimulation or inhibition affected the CD8+ T-cell exhaustion phenotype. Interference with STAT1 reduced the expression of HSP90α and impaired its regulation of CD8+ T cells. The Co-Immunoprecipitation results indicated that HSP90α can directly or indirectly bind to TOX to regulate TOX expression and downstream signal transduction. In summary, by inhibiting TOX-mediated exhaustion signaling pathways, HSP90α inhibited CD8+ T-cell exhaustion in ALI. The participation of STAT1 in the regulation of HSP90α was required.
Pyrotinib and trastuzumab plus palbociclib and fulvestrant in HR+/HER2+ breast cancer patients with brain metastasis
Authors: Chen Dongshao , Xu Fei , Lu Yongkui , Xia Wen , Du Caiwen , Xiong Dun , Song Dong , Shi Yanxia , Yuan Zhongyu , Zheng Qiufan , Jiang Kuikui , An Xin , Xue Cong , Huang Jiajia , Bi Xiwen , Chen Meiting , Zhang Jingmin , Wang Shusen , Hong Ruoxi 
Published: June 2024

AbstractHuman epidermal growth factor receptor 2-positive (HER2+) breast cancer (BC) patients are at a high risk of developing metastases in the brain. However, research focusing on treatment strategies for hormonal receptor positive (HR+), HER2+ BC patients with brain metastases (BM) remains limited. Thus, a multi-center, prospective trial was conducted in China. Women over the age of 18 who were naive to whole brain radiotherapy and had estrogen receptor (ER)/progesterone-receptor (PgR) positive, HER2+ BM were treated with palbociclib, fulvestrant, trastuzumab and pyrotinib, until disease progression or the development of intolerable side effects. The primary endpoint was objective response rate (ORR) in the central nervous system (CNS). This ongoing study is still recruiting participants and is registered with ClinicalTrials.gov (NCT04334330). This report presents the findings from an interim analysis. From December 4, 2020, to November 2, 2022, 15 patients were enrolled. Among the 14 patients who were evaluable for clinical response, the ORR was 35.7% (95% CI: 12.8–64.9%), with a CNS–ORR of 28.6% (95% CI: 8.4–58.1%). The median follow-up period was 6.3 months (range, 2.1–14.3 months), during which the median progression-free survival (PFS) was 10.6 months (95% CI: 4.3–16.9 months), and the median time to CNS progression was 8.5 months (95% CI: 5.9–11.1 months). The most common adverse event was diarrhea (93%), with 33% having grade 3 and 6.7% having grade 4. The study suggests that the combination of palbociclib, trastuzumab, pyrotinib and fulvestrant offers a promising chemo-free treatment strategy for HR+, HER2+ BC patients with BM.
A high-quality dataset featuring classified and annotated cervical spine X-ray atlas
Authors: Ran Yu , Qin Wanli , Qin Changlong , Li Xiaobin , Liu Yixing , Xu Lin , Mu Xiaohong , Yan Li , Wang Bei , Dai Yuxiang , Chen Jiang , Han Dongran 
Published: June 2024

AbstractRecent research in computational imaging largely focuses on developing machine learning (ML) techniques for image recognition in the medical field, which requires large-scale and high-quality training datasets consisting of raw images and annotated images. However, suitable experimental datasets for cervical spine X-ray are scarce. We fill the gap by providing an open-access Cervical Spine X-ray Atlas (CSXA), which includes 4963 raw PNG images and 4963 annotated images with JSON format (JavaScript Object Notation). Every image in the CSXA is enriched with gender, age, pixel equivalent, asymptomatic and symptomatic classifications, cervical curvature categorization and 118 quantitative parameters. Subsequently, an efficient algorithm has developed to transform 23 keypoints in images into 77 quantitative parameters for cervical spine disease diagnosis and treatment. The algorithm’s development is intended to assist future researchers in repurposing annotated images for the advancement of machine learning techniques across various image recognition tasks. The CSXA and algorithm are open-access with the intention of aiding the research communities in experiment replication and advancing the field of medical imaging in cervical spine.
A precise performance-based reimbursement model for the multi-centre NAPKON cohorts – development and evaluation
Authors: Appel Katharina S. , Lee Chin Huang , Nunes de Miranda Susana M. , Maier Daniel , Reese Jens-Peter , Anton Gabriele , Bahmer Thomas , Ballhausen Sabrina , Balzuweit Beate , Bellinghausen Carla , Blumentritt Arne , Brechtel Markus , Chaplinskaya-Sobol Irina , Erber Johanna , Fiedler Karin , Geisler Ramsia , Heyder Ralf , Illig Thomas , Kohls Mirjam , Kollek Jenny , Krist Lilian , Lorbeer Roberto , Miljukov Olga , Mitrov Lazar , Nürnberger Carolin , Pape Christian , Pley Christina , Schäfer Christian , Schaller Jens , Schattschneider Mario , Scherer Margarete , Schulze Nick , Stahl Dana , Stubbe Hans Christian , Tamminga Thalea , Tebbe Johannes Josef , Vehreschild Maria J. G. T. , Wiedmann Silke , Vehreschild Jörg Janne 
Published: June 2024

AbstractFair allocation of funding in multi-centre clinical studies is challenging. Models commonly used in Germany - the case fees (“fixed-rate model”, FRM) and up-front staffing and consumables (“up-front allocation model”, UFAM) lack transparency and fail to suitably accommodate variations in centre performance. We developed a performance-based reimbursement model (PBRM) with automated calculation of conducted activities and applied it to the cohorts of the National Pandemic Cohort Network (NAPKON) within the Network of University Medicine (NUM). The study protocol activities, which were derived from data management systems, underwent validation through standardized quality checks by multiple stakeholders. The PBRM output (first funding period) was compared among centres and cohorts, and the cost-efficiency of the models was evaluated. Cases per centre varied from one to 164. The mean case reimbursement differed among the cohorts (1173.21€ [95% CI 645.68–1700.73] to 3863.43€ [95% CI 1468.89–6257.96]) and centres and mostly fell short of the expected amount. Model comparisons revealed higher cost-efficiency of the PBRM compared to FRM and UFAM, especially for low recruitment outliers. In conclusion, we have developed a reimbursement model that is transparent, accurate, and flexible. In multi-centre collaborations where heterogeneity between centres is expected, a PBRM could be used as a model to address performance discrepancies.Trial registration: https://clinicaltrials.gov/ct2/show/NCT04768998; https://clinicaltrials.gov/ct2/show/NCT04747366; https://clinicaltrials.gov/ct2/show/NCT04679584.
Patients with gynecological malignancies are similar to other IVF patients without cancer for clinical and molecular reproductive parameters and DNA damage response pattern
Authors: Esmaeilian Yashar , Yusufoglu Sevgi , İltumur Ece , Cimen Deniz Ugurlu , Vatansever Dogan , Taskiran Cagatay , Turan Volkan , Yakin Kayhan , İncir Said , Urman Bulent , Oktem Ozgur 
Published: June 2024

AbstractThis study intended to investigate if gynecological cancers compromise ovarian function and reduce the success of assisted reproduction techniques (ART). No clinical and molecular data together is available on this issue for gynecological or other organ cancers. Steroidogenic pathways and DNA damage response characteristics of the granulosa cells retrieved from the 39 gynecological cancer patients were analyzed together with their clinical ART characteristics in comparison to 31 control ART patients. Patients with gynecological malignancies were similar to the control IVF patients for the number of mature oocytes retrieved, fertilization rates and embryo development competency. Molecular analyses of the granulosa cells retrieved from these cancer patients did not detect any perturbations in gonadotropin receptor expression and response, sex steroid production, cholesterol utilization/storage and, DNA damage response pattern in comparison to control IVF patients without cancer. This study provides the first reassuring clinical and molecular combined data set that the presence of gynecological malignancy does not appear to have any detrimental effect on clinical IVF cycle characteristics and ovarian functioning at molecular level.
Increase in forest structural complexity along a precipitation gradient is mediated by partial harvests in temperate Patagonian forests
Authors: Soto Daniel P. , Seidel Dominik , Hernández-Moreno Ángela , Puettmann Klaus J. , Donoso Pablo J. 
Published: June 2024

AbstractIncreasing forest structural complexity is becoming a common goal in forestry worldwide. However, the lack of empirical quantification clouds its implementation. Here we quantified the long-term effects (> 30 y) of partial harvest on stand structural complexity and net primary productivity using the east–west precipitation gradient (318–2508 mm, mean annual precipitation-MAP) of western Patagonian as a study system. In this gradient, pairs of 1-ha plots on 20 sites (20 plots harvested and 20 plots unharvested) were installed. In each plot terrestrial laser scanning was used to quantify the stand structural complexity index (SSCI), and Sentinel satellite images to obtain the Enhanced Vegetation Index (EVI: proxy of net primary productivity). Generalized linear mixed-effect models were used to relate SSCI to MAP and EVI to SSCI, with harvesting as indicator variable, and site as random variable (two plots nested to same precipitation). Results showed that harvested plots on mesic-to-humid sites (but not on dry sites) had higher SSCI and EVI values compared to unharvested plots, likely due to a greater vertical canopy packing. These results show the influence of precipitation on SSCI, which resulted in a more diversified stand structure and higher EVI. Such insights support site-specific management aimed to increase forest structural complexity.
High-resolution adaptive optics-trans-scleral flood illumination (AO-TFI) imaging of retinal pigment epithelium (RPE) in central serous chorioretinopathy (CSCR)
Authors: Govindahari Vishal , Dornier Rémy , Ferdowsi Sohrab , Moser Christophe , Mantel Irmela , Behar-Cohen Francine , Kowalczuk Laura 
Published: June 2024

AbstractThis study aims to correlate adaptive optics-transscleral flood illumination (AO-TFI) images of the retinal pigment epithelium (RPE) in central serous chorioretinopathy (CSCR) with standard clinical images and compare cell morphological features with those of healthy eyes. After stitching 125 AO-TFI images acquired in CSCR eyes (including 6 active CSCR, 15 resolved CSCR, and 3 from healthy contralateral), 24 montages were correlated with blue-autofluorescence, infrared and optical coherence tomography images. All 68 AO-TFI images acquired in pathological areas exhibited significant RPE contrast changes. Among the 52 healthy areas in clinical images, AO-TFI revealed a normal RPE mosaic in 62% of the images and an altered RPE pattern in 38% of the images. Morphological features of the RPE cells were quantified in 54 AO-TFI images depicting clinically normal areas (from 12 CSCR eyes). Comparison with data from 149 AO-TFI images acquired in 33 healthy eyes revealed significantly increased morphological heterogeneity. In CSCR, AO-TFI not only enabled high-resolution imaging of outer retinal alterations, but also revealed RPE abnormalities undetectable by all other imaging modalities. Further studies are required to estimate the prognosis value of these abnormalities. Imaging of the RPE using AO-TFI holds great promise for improving our understanding of the CSCR pathogenesis.
Minimization of heavy metal toxicity in radish (Raphanus sativus) by strigolactone and biochar
Authors: Shahzad Khurram , Danish Subhan , Mubeen Sidra , Dawar Khadim , Fahad Shah , Hasnain Zuhair , Ansari Mohammad Javed , Almoallim Hesham S. 
Published: June 2024

AbstractDue to the high solubility of Cd in water, it is considered a potential toxin which can cause cancer in humans. In plants, it is associated with the development of oxidative stress due to the generation of reactive oxygen species. To overcome this issue, the roles of different plant hormones are vital. Strigolactones, one of such natural plant hormones, show promise in alleviating cadmium toxicity by mitigating its harmful effects. Acidified biochar (AB) can also effectively mitigate cadmium toxicity via ion adsorption and pH buffering. However, the combined effects of strigolactone and AB still need in-depth investigations in the context of existing literature. This study aimed to assess the individual and combined impacts of SLs (0 and 25 µM) and AB (0 and 0.75% w/w) on radish growth under Cd toxicity, i.e., 0 and 20 mg Cd/kg soil. Using a fully randomized design (CRD), each treatment was administered in four replicates. In comparison to the control under 20 mg Cd/kg soil contamination, the results showed that 25 µM strigolactone + 0.75% AB significantly improved the following: radish shoot length (~ 17%), root length (~ 47%), plant fresh weight (~ 28%), plant dry weight (~ 96%), chlorophyll a (~ 43%), chlorophyll b (~ 31%), and total chlorophyll (~ 37%). It was also noted that 0.75% AB was more pronounced in decreasing antioxidant activities than 25 µM strigolactone under 20 mg Cd/ kg soil toxicity. However, performing 25 µM strigolactone + 0.75% AB was far better than the sole application of 25 µM strigolactone and 0.75% AB in decreasing antioxidant activities in radish plants. In conclusion, by regulating antioxidant activities, 25 µM strigolactone + 0.75% AB can increase radish growth in cadmium-contaminated soils.
Diffusion model-based probabilistic downscaling for 180-year East Asian climate reconstruction
Authors: Ling Fenghua , Lu Zeyu , Luo Jing-Jia , Bai Lei , Behera Swadhin K. , Jin Dachao , Pan Baoxiang , Jiang Huidong , Yamagata Toshio 
Published: June 2024

AbstractAs our planet is entering into the “global boiling” era, understanding regional climate change becomes imperative. Effective downscaling methods that provide localized insights are crucial for this target. Traditional approaches, including computationally-demanding regional dynamical models or statistical downscaling frameworks, are often susceptible to the influence of downscaling uncertainty. Here, we address these limitations by introducing a diffusion probabilistic downscaling model (DPDM) into the meteorological field. This model can efficiently transform data from 1° to 0.1° resolution. Compared with deterministic downscaling schemes, it not only has more accurate local details, but also can generate a large number of ensemble members based on probability distribution sampling to evaluate the uncertainty of downscaling. Additionally, we apply the model to generate a 180-year dataset of monthly surface variables in East Asia, offering a more detailed perspective for understanding local scale climate change over the past centuries.
Crucial role of sea surface temperature warming patterns in near-term high-impact weather and climate projection
Authors: Zhao Ming , Knutson Thomas 
Published: June 2024

AbstractRecent studies indicate that virtually all global climate models (GCMs) have had difficulty simulating sea surface temperature (SST) trend patterns over the past four decades. GCMs produce enhanced warming in the eastern Equatorial Pacific (EPAC) and Southern Ocean (SO) warming, while observations show intensified warming in the Indo-Pacific Warm Pool (IPWP) and slight cooling in the eastern EPAC and SO. Using Geophysical Fluid Dynamics Laboratory’s latest higher resolution atmospheric model and coupled prediction system, we show the model biases in SST trend pattern have profound implications for near-term projections of high-impact storm statistics, including the frequency of atmospheric rivers (AR), tropical storms (TS) and mesoscale convection systems (MCS), as well as for hydrological and climate sensitivity. If the future SST warming pattern continues to resemble the observed pattern from the past few decades rather than the GCM simulated/predicted patterns, our results suggest (1) a drastically different future projection of high-impact storms and their associated hydroclimate changes, especially over the Western Hemisphere, (2) a stronger global hydrological sensitivity, and (3) substantially less global warming due to stronger negative feedback and lower climate sensitivity. The roles of SST trend patterns over the EPAC, IPWP, SO, and the North Atlantic tropical cyclone Main Development Region (AMDR) are isolated, quantified, and used to understand the simulated differences. Specifically, SST trend patterns in the EPAC and AMDR are crucial for modeled differences in AR and MCS frequency, while those in the IPWP and AMDR are essential for differences in TS frequency over the North Atlantic.
U-Pb geochronology reveals that hydrothermal dolomitization was coeval to the deposition of the Burgess Shale lagerstätte
Authors: McCormick Cole A. , Corlett Hilary , Roberts Nick M. W. , Johnston Paul A. , Collom Christopher J. , Stacey Jack , Koeshidayatullah Ardiansyah , Hollis Cathy 
Published: June 2024

AbstractFault-controlled, hydrothermal dolomitization often occurs at margins between shallow-water carbonate platforms and deep-water sedimentary basins. In western Canada, for example, the platform margin between the Cathedral Formation and the Burgess Shale Formation has been dolomitized at temperatures up to ~200 °C, with local magnesite, talc, and clinochlore mineralization. At the same time, the Burgess Shale Formation includes exceptional fossils that provide key evidence of the radiation of the animal phyla during the Cambrian Period (541 to 485.4 Ma). This lagerstätte and Mg-rich minerals within the adjacent and underlying strata, however, have been critically understudied. Here we show, using carbonate U-Pb geochronology, that western Canada was tectonically active and subject to hydrothermal dolomitization during the Middle Cambrian (Miaolingian Epoch) to Middle Ordovician (488.1 ± 18.8 Ma). These results extend the latest stages of rifting along the western margin of Laurentia into the Paleozoic, while also suggesting that the dolomitization of the Cathedral Formation occurred at the same time as the deposition of the Burgess Shale lagerstätte.
Integrated actions across multiple sustainable development goals (SDGs) can help address coastal ocean acidification
Authors: Vargas Cristian A. , Gelcich Stefan 
Published: June 2024

AbstractThe Sustainable Development Goals (SDGs) adopted by the United Nations in 2015 constitute a set of 17 global goals established as a blueprint for achieving a more sustainable and equitable world for humanity. As part of the SDGs, target 14.3 is focuses on minimizing and addressing the impacts of Ocean Acidification (OA). We argue that moving forward in meeting the targets related to pH levels in the coastal ocean can be facilitated through accounting for various drivers of pH change, which are associated with advancing a suite of SDG goals. Addressing ‘coastal acidification’ via a suite of linked SDGs may help avoid inaction through connecting global phenomena with local impacts and drivers. This in turn can provide opportunities for designing novel place-based actions or partnerships that can aid and provide synergies for the joint implementation of programs and policies that tackle a suite of SDGs and the specific targets related to coastal ocean pH.
Reconstruction of rough surfaces from a single receiver at grazing angle
Authors: Chen Yuxuan , Spivack Mark , Rath Spivack Orsola 
Published: June 2024

AbstractThis article develops a method for recovering a one‐dimensional rough surface profile from scattered wave field, using a single receiver and repeated measurements when the surface is moving with respect to source and receiver. This extends a previously introduced marching method utilizing low grazing angles, and addresses the key issue of the requirement for many simultaneous receivers. The algorithm recovers the surface height below the receiver point step‐by‐step as the surface is moved, using the parabolic wave integral equations. Numerical examples of reconstructed surfaces demonstrate that the method is robust in both Dirichlet and Neumann boundary conditions, and with respect to different roughness characteristics and measurement noise.
Scattering properties of protoplanetary dust analogs with microwave analogy: Rough compact grains
Authors: Tobon Valencia Vanesa , Geffrin Jean-Michel , Ménard François , Milli Julien , Renard Jean-Baptiste , Tortel Hervé , Litman Amélie 
Published: June 2024

Context. Scattering simulations of perfect spheres are not sufficient to explain the observations of scattered light from protoplanetary and debris disks, especially when the dust sizes are on the same order of magnitude as the wavelength used to perform the observations. Moreover, examples of grains collected from the Solar System have proved that the morphology of interstellar dust is irregular. These pieces of evidence lead us to consider that the morphologies of the dust that participates in these circumstellar disks are more complex than those of spheres.
Aims. We aim to measure and simulate the scattering properties of six rough compact grains to identify how their morphology affects their scattering properties. These grains are intended to be dust analogs of protoplanetary and debris disks. Their convexity ranges from 75% to 99%.
Methods. Grains were 3D printed using stereolithography, and their shape and refractive index were controlled. These analogs were measured with our microwave-scattering experiment (microwave analogy) at wavelengths ranging from 16.7 mm to 100 mm, leading to size parameters from X = 1.07 to X = 7.73. In parallel, their scattering properties were simulated with our finite-element method (FEM), which contained the same geometric file as the 3D printed grains.
Results. We retrieved five scattering properties of these grains, that is, the phase function, the degree of linear polarization (DLP), and three other Mueller matrix elements 〈Sij〉. Two types of studies were performed. First, a study of the scattering properties averaged over several orientations of grains at different wavelengths. Second, a study of the same scattering properties, for which a power-law size distribution effect was applied.
Conclusions. The very good correspondence between the measured and simulated Mueller matrix elements demonstrated the accuracy of our measurement setup and the efficiency of our FEM simulations. For the first study, DLP proved to be a good indicator of the grain morphology in terms of convexity and shape anisotropy. For the second study, backscattering enhancements of the phase function were related to the grain convexity. The maximum DLP and its negative polarization branches as well as the 〈S22〉/〈S11〉 levels were related to the shape anisotropy of our grains.