bluuebunny/crossref_metadata_2025_split huggingface dataset:
LINC00173 silence and estrone supply suppress ER+ breast cancer by estrogen receptor α degradation and LITAF activation
Authors: Xie Yu , Shan Meihua , Yu Jing , Du Yongjun , Wu Chengkun , Liu Shujing , Li Jiayin , Xiao Yupeng , Yan Yan , Li Ning , Qin Junfang , Lan Lan , Wang Yue 
Published: May 2024

AbstractPersistent activation of estrogen receptor alpha (ERα)‐mediated estrogen signaling plays a pivotal role in driving the progression of estrogen receptor positive (ER+) breast cancer (BC). In the current study, LINC00173, a long non‐coding RNA, was found to bind both ERα and lipopolysaccharide (LPS)‐induced tumor necrosis factor alpha (TNFα) factor (LITAF), then cooperatively to inhibit ERα protein degradation by impeding the nuclear export of ERα. Concurrently, LITAF was found to attenuate TNFα transcription after binding to LINC00173, and this attenuating transcriptional effect was quite significant under lipopolysaccharide stimulation. Distinct functional disparities between estrogen subtypes emerge, with estradiol synergistically promoting ER+ BC cell growth with LINC00173, while estrone (E1) facilitated LITAF‐transcriptional activation. In terms of therapeutic significance, silencing LINC00173 alongside moderate addition of E1 heightened TNFα and induced apoptosis, effectively inhibiting ER+ BC progression.
The reflections of health service providers on implementing contingency management for methamphetamine use disorder in Australia
Authors: Clay Simon , Wilkinson Zachary , Ginley Meredith , Arunogiri Shalini , Christmass Michael , Membrey Dean , MacCartney Paul , Sutherland Rachel , Colledge‐Frisby Samantha , Marshall Alison D. , Nagle Jack , Degenhardt Louisa , Farrell Michael , McKetin Rebecca 
Published: May 2024

AbstractIntroductionContingency management (CM) is the most effective treatment for reducing methamphetamine use. We sought to understand why CM has not been taken up to manage methamphetamine use disorder in Australia.MethodsSix focus groups (4–8 participants per group) were conducted with health workers from agencies in Australia that provided drug‐related health care to people who use methamphetamine. These agencies had no previous experience delivering CM for substance use. The potential acceptability and feasibility of implementing CM in their services were discussed.ResultsParticipants felt that it would be beneficial to have an evidence‐based treatment for methamphetamine use disorder. This sentiment was offset by concerns that CM conflicted with a client‐centred harm‐reduction approach and that it dictated the goal of treatment as abstinence. It was also perceived as potentially coercive and seen to reify the power imbalance in the therapeutic relationship and therefore potentially reinforce stigma. There was also concern about the public's perception and the political acceptability of CM, who would fund CM, and the inequity of providing incentives only to clients with a methamphetamine use disorder. Some concerns could be ameliorated if the goals and structure of CM could be tailored to a client's needs.Discussion and ConclusionsMany healthcare workers were keen to offer CM as an effective treatment option for people with methamphetamine use disorder, but CM would need to be sufficiently flexible to allow it to be tailored to client needs and implemented in a way that did not adversely impact the therapeutic relationship.
Memory, mood and associated neuroanatomy in individuals with steroid sulphatase deficiency (X‐linked ichthyosis)
Authors: Wren Georgina H. , Flanagan Jessica , Underwood Jack F. G. , Thompson Andrew R. , Humby Trevor , Davies William 
Published: May 2024

AbstractSteroid sulphatase (STS) cleaves sulphate groups from steroid hormones, and steroid (sulphate) levels correlate with mood and age‐related cognitive decline. In animals, STS inhibition or deletion of the associated gene, enhances memory/neuroprotection and alters hippocampal neurochemistry. Little is known about the consequences of constitutive STS deficiency on memory‐related processes in humans. We investigated self‐reported memory performance (Multifactorial Memory Questionnaire), word‐picture recall and recent mood (Kessler Psychological Distress Scale, K10) in adult males with STS deficiency diagnosed with the dermatological condition X‐linked ichthyosis (XLI; n = 41) and in adult female carriers of XLI‐associated genetic variants (n = 79); we compared results to those obtained from matched control subjects [diagnosed with ichthyosis vulgaris (IV, n = 98) or recruited from the general population (n = 250)]. Using the UK Biobank, we compared mood/memory‐related neuroanatomy in carriers of genetic deletions encompassing STS (n = 28) and non‐carriers (n = 34,522). We found poorer word‐picture recall and lower perceived memory abilities in males with XLI and female carriers compared with control groups. XLI‐associated variant carriers and individuals with IV reported more adverse mood symptoms, reduced memory contentment and greater use of memory aids, compared with general population controls. Mood and memory findings appeared largely independent. Neuroanatomical analysis only indicated a nominally‐significantly larger molecular layer in the right hippocampal body of deletion carriers relative to non‐carriers. In humans, constitutive STS deficiency appears associated with mood‐independent impairments in memory but not with large effects on underlying brain structure; the mediating psychobiological mechanisms might be explored further in individuals with XLI and in new mammalian models lacking STS developmentally.
Monitoring anti‐Xa levels in elderly medical patients undergoing thromboprophylaxis: A prospective cohort study
Authors: Papazachariou Andria , Papadakis John A , Malikides Vironas , Nikiforou Antigoni , Alexiadou Dimitra , Malikides Onoufrios , Ioannou Petros , Ximeri Maria , Kofteridis Diamantis P. 
Published: May 2024

IntroductionGiven the rising incidence of venous thromboembolism (VTE) and insufficient thromboprophylaxis dosing evidence in certain patients, the precise monitoring of anti‐Xa (aFXa) levels is crucial. The aim of this study is to investigate the achievement of prophylactic aFXa levels in medical inpatients who were receiving parenteral anticoagulant and to evaluate the impact of various factors on aFXa levels.MethodsThis is a single‐center observational cohort study conducted on patients admitted to the Department of Internal Medicine at the University Hospital of Heraklion, Greece, from March to August 2023. These individuals received low‐molecular‐weight heparins thromboprophylaxis owing to an increased risk of VTE. Data regarding demographics, past medical history, and somatometric and laboratory findings were recorded. The established range for peak prophylactic aFXa levels was defined as 0.2–0.5 IU/mL.ResultsIn this study, we enrolled 150 individuals [91 (60.7%) women] with a mean age of 80.0 ± 14.1 years. Sixty‐two (41.4%) patients exhibited non‐prophylactic peak aFXa levels. Supratherapeutic levels were observed in all underweight patients and subtherapeutic levels in 12 of 13 obese patients in class II and III. A multivariate linear regression analysis revealed that body weight, cancer, and the Charlson Comorbidity Index (CCI) were independent factors influencing aFXa levels.ConclusionsOur study reveals a substantial portion of medical elderly inpatients on thromboprophylaxis with non‐prophylactic aFXa levels, with a notable prevalence among underweight and severely obese patients. Body weight, cancer, and CCI were identified as independent factors influencing aFXa levels, advocating for tailored thromboprophylaxis strategies. Further research is warranted to validate personalized dosing approaches and to enhance clinical decision‐making. Geriatr Gerontol Int 2024; 24: 587–594.
Evaluation of the Oral Hygiene Ability Instrument (OHAI): Test of reliability
Authors: Grönbeck Lindén Ingela , Andersson Pia , Dahlin Ivanoff Synneve , Gahnberg Lars , Hägglin Catharina 
Published: May 2024

AbstractObjectivesTo evaluate the reliability of the new Oral Hygiene Ability Instrument (OHAI) developed to assess the cause of any inability for older adults to manage oral hygiene self‐care. Oral hygiene is an important part of oral health. The inability to manage oral hygiene, combined with other risk factors, often results in poor oral health and impaired quality of life. A reliable OHAI could benefit preventive oral health care.MethodsThe preliminary OHAI uses 33 items in three parts: (I) interview, (II) clinical examination and (III) observation of oral hygiene activities. A total of 37 older adults participated in a test‐retest study of Part I. Inclusion criteria were age ≥ 65 years, have at least one tooth and be able to oral hygiene self‐care. The intra‐ and inter‐rater reliability of parts II and III used films and photographs of OHAI assessments of 15 different individuals. These films/photographs were assessed twice by four dental professionals who had participated in the OHAI data collection. For statistical analyses of the reliability, per cent agreement, Krippendorff's alpha and E. Svensson's method were used.ResultsThe test‐retest for Part I items showed acceptable‐to‐good agreement and no systematic disagreement. In Part II, two items showed somewhat limited reliability. Part III showed good intra‐ and inter‐rater reliability.ConclusionThe OHAI items seem stable and reliable for the intended sample, and the instrument may be a valuable tool to identify older adults at risk of impaired oral health. However, two items may need to be reformulated.
Effect of irradiation intensity on the rate of photocatalysis of TiO2 coatings obtained by detonation spraying
Authors: Sirota V. V. , Savotchenko S. E. , Strokova V. V. , Vashchilin V. S. , Podgornyi D. S. , Limarenko M. V. , Kovaleva M. G. 
Published: May 2024

AbstractThe influence of UV irradiation intensity on the rate constant of photocatalytic reaction of TiO2 coatings applied by detonation spraying on the hot‐rolled carbon steel and glass‐cloth‐base laminate (plastic) is studied. The effect of substrate material on the studied phenomenon is pronounced. The kinetic curves at different UV irradiation intensities and the rate constants of the photocatalytic reaction are obtained for different values of energy flux density varied from 3.0 to 8.0 mW/m2. The photocatalytic rate constant of coatings placed on plastic increases with an increase in the energy flux density from.038 to.055 1/h. Samples applied at a spray distance of 50 mm are characterized by a higher the photocatalytic rate constant than those applied at a distance of 40 mm. The value of photocatalytic rate constant does not change starting from a certain flow value for such samples and reaches the saturation value of about.054 1/h. Saturation is achieved quite quickly already at a density flux of about 5.5 mW/m2. Two new phenomenological models based on differential equation, the analytical solutions to which sufficiently describe experimental dependence of the photocatalytic rate constant on the energy flux density, are proposed.
Highly crystalline yttria‐stabilized zirconia and titania thin films on plastic substrates via sol‐gel transfer process
Authors: Kozuka Hiromitsu , Yamada Takehito , Okuda Ryota , Hirakoso Hideyuki 
Published: May 2024

AbstractWe prepared yttria‐stabilized zirconia (YSZ) and titania crystalline thin films on polycarbonate (PC) substrates by the sol‐gel transfer technique that we have developed recently. Precursor gel films were deposited by spin‐coating on a polyimide/polyvinylpyrrolidone mixture layer on Si(100) substrates, and then were calcined at 600°C. The spin‐coating and the calcination were cycled, followed by final firing at 600–1000°C, to obtain 140–190 nm thick, YSZ and titania crystalline thin films. The film densification progressed as the final firing temperature increased, where the porosity calculated from the refractive index decreased down to a few percent. The film thus obtained was heated at 190°C on a hot plate, and a PC substrate was pressed on the film to transfer it to the PC substrate. The transferability quantified by image analysis of the film area was found to be significantly different between the YSZ and titania films. In the case of YSZ films, the area of silicon that was fractured and adhered to the transferred film increased significantly with increasing firing temperature, leading to a decrease down to 0% in the fraction of the successful transfer area without such a damage. On the other hand, the fraction of the successful transfer area remained around 90% irrespective of the firing temperature for the titania films. The surface of the titania films that were fired at 800–1000°C and transferred to PC substrates had voids of several tens nm scale, which may have been formed during anatase‐to‐rutile phase transformation. We thought that the reduced titania/Si(100) contact area due to such void formation facilitated the delamination of the titania films from Si(100) substrates. High temperature firing, which promotes crystallization and densification of films, thus affects differently the film transferability to plastic substrates, depending on the type of films. The work also suggested possible use of titania films as release‐assisting layers.
Accelerating B‐site cation ordering‐disordering transition and improving dielectric properties of Ba(Co,Zn)1/3Nb2/3O3 complex perovskite microwave ceramics by CuO doping
Authors: Li Hao , Liu Fei , Chang Kuan‐Chang , Lin Xinnan , Liu Shaojun 
Published: May 2024

AbstractThe B‐site cation ordering‐disordering transition process of complex perovskite Ba(B′1/3B″2/3)O3‐based microwave ceramics can be directly promoted by accelerating the mass transport process rather than depending on long‐time annealing treatment. Clear evidences suggest that CuO doping is helpful in enhancing the densification process of Ba(Co0.7Zn0.3)1/3Nb2/3O3 (BCZN) ceramics and promoting the growth and coarsening of the ordering domain, facilitated by the formation of quasi‐liquid CuO‐xCu2O layers. The improvement of the 1:2 ordering degree is attributed to the accelerated mass transport process enabled by quasi‐liquid CuO‐xCu2O layers rather than long‐time annealing treatment. Consequently, the Q×f values experience a significant increase from 21,200 to 69,300 GHz, owing to enhancements in the relative density and 1:2 ordering degree. CuO‐doped BCZN ceramics achieve high 1:2 ordering degree and Q×f values at relatively low temperatures and for short periods of time, and more importantly, it circumvents conventional lengthy annealing processes (>30 h) effectively to promote its ordering transition. These findings underscore the potential of accelerating mass transport process to promote ordering‐disordering phase transitions and improving dielectric properties of complex perovskite Ba(B′1/3B″2/3)O3‐based microwave ceramics.
Experiments in supply chain management research: A systematic review and future directions
Authors: Carter Craig R. , Rockwood Renae F. , Patel Pankaj C. , Bachrach Daniel , Bendoly Elliot , DuHadway Scott , Kaufmann Lutz 
Published: May 2024

AbstractThe supply chain management discipline has seen a tremendous growth in the use of experimental methods. Given the large number of published studies, the time seems opportune to systematically review the use of such approaches. In this note, we consider multiple dimensions of experimental design used in articles published in six of our premier journals. We present these findings and contemplate opportunities for future applications of experimental methods. In particular, we highlight a need to more regularly conduct and report on the results of power analyses and experimental checks, more carefully contemplate the justification and use of WEIRD (Western, educated, industrial, rich, and democratic) participants, develop and test mediated theoretical models, and increase our focus on teams as the unit of analysis when using experimental methods.
Platelets as an inter‐player between hyperlipidaemia and atherosclerosis
Authors: Li Nailin 
Published: May 2024

AbstractPlatelet hyperreactivity and hyperlipidaemia contribute significantly to atherosclerosis. Thus, it is desirable to review the platelet–hyperlipidaemia interplay and its impact on atherogenesis. Native low‐density lipoprotein (nLDL) and oxidized LDL (oxLDL) are the key proatherosclerotic components of hyperlipidaemia. nLDL binds to the platelet‐specific LDL receptor (LDLR) ApoE‐R2′, whereas oxLDL binds to the platelet‐expressed scavenger receptor CD36, lectin‐type oxidized LDLR 1 and scavenger receptor class A 1. Ligation of nLDL/oxLDL induces mild platelet activation and may prime platelets for other platelet agonists. Platelets, in turn, can modulate lipoprotein metabolisms. Platelets contribute to LDL oxidation by enhancing the production of reactive oxygen species and LDLR degradation via proprotein convertase subtilisin/kexin type 9 release. Platelet‐released platelet factor 4 and transforming growth factor β modulate LDL uptake and foam cell formation. Thus, platelet dysfunction and hyperlipidaemia work in concert to aggravate atherogenesis. Hypolipidemic drugs modulate platelet function, whereas antiplatelet drugs influence lipid metabolism. The research prospects of the platelet–hyperlipidaemia interplay in atherosclerosis are also discussed.
Feeding behaviour related to different feeding devices
Authors: Greppi Martina , Bordin Clara , Raspa Federica , Maccone Erica , Harris Patricia , Ellis Andrea Dorothea , Cavallini Damiano , Bergero Domenico , Valle Emanuela 
Published: May 2024

AbstractSlow feeding devices (SFDs) are useful tools in order to improve the horse well‐being and to reduce wastage, but their use may result in unnatural posture during feeding and frustration behaviours. Moreover, it may be important to evaluate the laterality during feeding. The aim of the study was to investigate ponies' feeding behaviour (i.e., lateralisation, frustration, postural eating style) with different hay feeding methods: on the ground (G), a fully filled hay net (HF), a partially filled hay net (HL), and a slow feeder hay box (HB). Nine ponies of two different breed types (Shetland and Welsh/Cob) were evaluated. Video recordings were obtained and then behavioural analysis was conducted. Data were analysed statistically using k‐means cluster analysis, repeated measurement mixed model procedure, principal component analysis (PCA) and Pearson's correlation coefficient. Head tilting was more often performed during feeding from HF and HL compared to G and HB. Forelimb forward, referring to the position that ponies assumed during feeding, was performed less frequently with the hay nets. Ponies, in particular Welsh/Cobs, tended to be more left‐lateralized and performed more backward ears when feeding from the hay nets. Blink rate was observed to be greater when ponies were fed from G. No head pushing, striking with forelimb and pawing while feeding was seen. Animals fed from HF tended to eat from the top of the hay net and consequently showed an arched neck; while HL and HB showed for elongated neck an intermediate pattern between G and HF. From our results, HB seemed to be a useful compromise between natural feeding on the ground and the use of hay nets.
Intergeneric hybrid origin of the invasive tetraploid Cirsium vulgare
Authors: Bureš P. , Del Guacchio E. , Šmerda J. , Özcan M. , Blizňáková P. , Vavrinec M. , Michálková E. , Veselý P. , Veselá K. , Zedek F. 
Published: May 2024

ABSTRACT

The invasive tetraploid Cirsium vulgare hybridizes with both Cirsium and Lophiolepis. Its conflicted position in molecular phylogenies, and its peculiar combination of morphological, anatomical, and genomic features that are alternatively shared with representatives of Cirsium or Lophiolepis, strongly suggest its intergeneric hybrid origin.

Genetic relationships of C. vulgare (8 samples) with genus Lophiolepis (11 species) and other representatives of genus Cirsium (12 species) were evaluated using restriction site‐associated DNA sequencing (RADseq) and examined using analytical and imaging approaches, such as NeighborNet, Heatmap, and STRUCTURE, to identify nuclear genomes admixture. Estimation of the intensity of spontaneous hybridization within and between Cirsium and Lophiolepis was based on herbarium revisions and published data for all reported hybrids pertinent to taxa currently included in Cirsium or Lophiolepis.

The genome of any examined Cirsium species is more similar to C. vulgare than to any Lophiolepis species, and vice versa. The nuclear genome of the tetraploid C. vulgare is composed of two equivalent parts, each attributable either to Lophiolepis or to Cirsium; the organellar RADseq data clustered C. vulgare with the genus Cirsium. Spontaneous hybridization between Cirsium and Lophiolepis is significantly less intensive than within these genera.

Our analyses provide compelling evidence that the invasive species C. vulgare has an allotetraploid intergeneric origin, with the maternal parent from Cirsium and the paternal from Lophiolepis. For the purpose of delimiting monophyletic genera, we propose keeping Lophiolepis separate from Cirsium and segregating C. vulgare into the hybridogenous genus Ascalea.
Loss of anoctamin 1 reveals a subtle role for BK channels in lymphatic muscle action potentials
Authors: Harlow Rebecca C. , Pea Grace A. , Broyhill Sarah E. , Patro Advaya , Bromert Karen H. , Stewart Randolph H. , Heaps Cristine L. , Castorena‐Gonzalez Jorge A. , Dongaonkar Ranjeet M. , Zawieja Scott D. 
Published: May 2024

AbstractCa2+ signalling plays a crucial role in determining lymphatic muscle cell excitability and contractility through its interaction with the Ca2+‐activated Cl− channel anoctamin 1 (ANO1). In contrast, the large‐conductance (BK) Ca2+‐activated K+ channel (KCa) and other KCa channels have prominent vasodilatory actions by hyperpolarizing vascular smooth muscle cells. Here, we assessed the expression and contribution of the KCa family to mouse and rat lymphatic collecting vessel contractile function. The BK channel was the only KCa channel consistently expressed in fluorescence‐activated cell sorting‐purified mouse lymphatic muscle cell lymphatic muscle cells. We used a pharmacological inhibitor of BK channels, iberiotoxin, and small‐conductance Ca2+‐activated K+ channels, apamin, to inhibit KCa channels acutely in ex vivo isobaric myography experiments and intracellular membrane potential recordings. In basal conditions, BK channel inhibition had little to no effect on either mouse inguinal–axillary lymphatic vessel (MIALV) or rat mesenteric lymphatic vessel contractions or action potentials (APs). We also tested BK channel inhibition under loss of ANO1 either by genetic ablation (Myh11CreERT2‐Ano1 fl/fl, Ano1ismKO) or by pharmacological inhibition with Ani9. In both Ano1ismKO MIALVs and Ani9‐pretreated MIALVs, inhibition of BK channels increased contraction amplitude, increased peak AP and broadened the peak of the AP spike. In rat mesenteric lymphatic vessels, BK channel inhibition also abolished the characteristic post‐spike notch, which was exaggerated with ANO1 inhibition, and significantly increased the peak potential and broadened the AP spike. We conclude that BK channels are present and functional on mouse and rat lymphatic muscle cells but are otherwise masked by the dominance of ANO1.
imageKey points
Mouse and rat lymphatic muscle cells express functional BK channels.
BK channels make little contribution to either rat or mouse lymphatic collecting vessel contractile function in basal conditions across a physiological pressure range.
ANO1 limits the peak membrane potential achieved in the action potential and sets a plateau potential limiting the voltage‐dependent activation of BK.
BK channels are activated when ANO1 is absent or blocked and slightly impair contractile strength by reducing the peak membrane potential achieved in the action potential spike and accelerating the post‐spike repolarization.