bluuebunny/crossref_metadata_2025_split huggingface dataset:
Surface immobilization of elastin‐like polypeptides using fluorinated surface modifying additives
Authors: Blit Patrick H. , Battiston Kyle G. , Woodhouse Kimberly A. , Santerre J. Paul 
Published: January 2011

AbstractElastin‐like polypeptide (ELP) surface modification represents a valuable approach for the development of biomaterials in a wide range of medical applications. In this study, ELP surface modification has been achieved through the use of elastin cross‐linking peptide (ECP) bioactive fluorinated surface modifiers (ECP‐BFSMs). The synthesis of low molecular weight fluorinated additives was described and their subsequent blending with a base polycarbonate urethane (PCNU) was shown to successfully enrich the surface to allow for ELP surface cross‐linking via lysine moieties on the peptide segments of the ECP‐BFSMs. The kinetics for the surface migration of fluorescent ECP‐BFSMs was studied over a 2‐week period by two‐photon confocal microscopy. A decrease in advancing contact angle from 87.9° to 75.3° was observed for ECP‐BFSM modified PCNU and was associated with the presence of ECP peptides on the surface. X‐ray photoelectron spectroscopy demonstrated an increase in surface atomic percent of fluorine (from 0.2 to 7.2%) and nitrogen (from 1.0 to 3.0%) associated with the surface localization of fluoro groups and amide groups associated with the peptides in the ECP‐BFSMs. A further increase in surface atomic percent of nitrogen (from 3.0 to 8.3%) was observed after ELP surface cross‐linking. These ELP‐modified surfaces were shown to promote increased smooth muscle cell adhesion, spreading and retention over a 7‐day culture period relative to their non‐ELP4 analogs. This novel surface modifying additive approach may be used for various biomimetic applications since it generates a stable ECM‐like surface retained onto a relatively inert fluorinated background. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
Successful preoperative treatment of a Graves' disease patient with agranulocytosis and hemophagocytosis using double filtration plasmapheresis
Authors: Lew Wei Han , Chang Chun‐Jen , Lin Jiunn‐Diann , Cheng Chung‐Yi , Chen Yin‐Kai , Lee Ting‐I 
Published: January 2011

AbstractAgranulocytosis is an uncommon but serious complication of Graves' disease under thionamide therapy. In some patients removal of circulating thyroid hormones and thyroid antibodies by plasmapheresis is an effective adjunctive therapeutic option. In perioperative settings, however, plasmapheresis may cause excess bleeding intraoperatively due to coagulation factor depletion unless fresh frozen plasma (FFP) products are used in the replacement fluid mix. Double filtration plasmapheresis (DFPP) in which only a small amount of albumin supplementation is used may be a potential alternative to conventional apheresis interventions where clotting factor depletion is problematic. We report a case of a patient with Graves' disease complicated with intravenous immunoglobulin responsive methimazole‐induced agranulocytosis/hemophagocytosis who underwent successful preoperative DFPP treatment in preparation for thyriodectomy. In addition to conventional apheresis using FFP replacement, DFPP may offer an effective adjunct option in the management of hyperthyroid patients needing emergent surgical interventions. J. Clin. Apheresis, 2011. © 2011 Wiley‐Liss, Inc.
Analysis of active components of rhinoceros, water buffalo and yak horns using two‐dimensional electrophoresis and ethnopharmacological evaluation
Authors: Liu Rui , Duan Jin‐ao , Wang Min , Shang Erxin , Guo Jianming , Tang Yuping 
Published: January 2011

AbstractCornu Rhinoceri Asiatici (rhinoceros horn, RH), Cornu Bubali (water buffalo horn, WBH), and Cornu Bovis grunniens (yak horn, YH) are traditional Chinese medicine (TCM), and have been used in China for thousands of years. In this study, ethnopharmacological experiments were used to evaluate and verify the traditional efficacies of horns. Area under curve (AUC) was used to quantify the pharmacological efficacy strength of three horns. Two‐dimensional electrophoresis (2‐DE) was used to analyze the protein components in horns, as a result, 14 common protein spots in rhinoceros horn, water buffalo horn, and yak horn electrophoresis gels were found by image analysis. Then linear regression analysis was used to establish the correlation between pharmacological efficacies and components in the horns, and five potential active components were selected from the 14 common protein spots. Finally, two protein spots from five were identified by matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS). In this study, a simple method to construct correlation between components and efficacy strength was explored by linear regression analysis, which could be applied to screen potential active components of animal horns.
Determination of bavachin and isobavachalcone in Fructus Psoraleae by high‐performance liquid chromatography with electrochemical detection
Authors: Li Yuan , Wang Fang , Chen Zilin 
Published: January 2011

AbstractA simple, sensitive and selective method of high‐performance liquid chromatography with electrochemical detection (HPLC‐ECD) has been developed for simultaneous determination of bavachin and isobavachalcone in Fructus Psoraleae. At optimized conditions, bavachin and isobavachalcone could be well separated within 15 min at a detection potential of +0.80 V with 0.03 mol/L acetate buffer solution (pH 5.17)/acetonitrile (2:3, v/v) as the mobile phase. The relationships between peak areas and concentrations were linear from 8.26×10−7 to 1.21×10−4 mol/L for bavachin, and from 1.01×10−8 to 1.61×10−4 mol/L for isobavachalcone, respectively. The method offered excellent linearity with regression coefficient R2>0.995. The method presented detection limits (S/N=3) of 8.81×10−9 mol/L for bavachin and 1.17×10−10 mol/L for isobavachalcone. It indicates that the sensitivity of electrochemical detection is ten times higher than that of diode array detection (DAD). The mean recoveries around 98% with a relative standard deviation less than 3.1% for the two analytes have been obtained. The proposed separation and detection procedures were successfully applied to the simultaneous determination of bavachin and isobavachalcone in traditional Chinese medicine.
Effects of media exposure on adolescents traumatized in a school shooting
Authors: Haravuori Henna , Suomalainen Laura , Berg Noora , Kiviruusu Olli , Marttunen Mauri 
Published: January 2011

AbstractThis study analyzes the impact of the media on adolescents traumatized in a school shooting. Participants were trauma‐exposed students (n = 231) and comparison students (n = 526), aged 13–19 years. A questionnaire that included the Impact of Event Scale and a 36‐item General Health Questionnaire was administered 4 months after the shooting. Being interviewed was associated with higher scores on the Impact of Event Scale (p = .005), but posttraumatic symptoms did not differ between those who refused to be interviewed and those not approached by reporters. Following a higher number of media outlets did not affect symptoms.
Cognitive processing, rumination, and posttraumatic growth
Authors: Stockton Hannah , Hunt Nigel , Joseph Stephen 
Published: January 2011

AbstractIntrusive thoughts about traumatic events are an important factor in determining the development of posttraumatic growth, although research has focused on the frequency rather than the type of intrusions. Based on cognitive processing models of posttraumatic growth, the authors present two cross‐sectional studies exploring different types of intrusive ruminations about trauma and their associations with posttraumatic growth. Study 1 examines brooding and reflection; Study 2 uses measures of both deliberate and intrusive rumination regarding a past trauma. The results indicate that intrusive reexperiencing and ruminative brooding are not significantly associated with posttraumatic growth, whereas deliberate rumination is significantly positively associated with posttraumatic growth (β = .44, p < .001) and reflection (partial r = .20, p = .003) is positively associated with posttraumatic growth in the context of low brooding.
The familial influence of loss and trauma on refugee mental health: A multilevel path analysis
Authors: Nickerson Angela , Bryant Richard A. , Brooks Robert , Steel Zachary , Silove Derrick , Chen Jack 
Published: January 2011

AbstractAlthough the impact of human rights violations on the mental health of refugees has been well documented, little is known about these effects at a family level. In this study the authors examined the relationships among loss, trauma, and mental health at the individual and family levels in resettled Mandaean refugees (N = 315). Trauma, loss, posttraumatic stress disorder, depression, complicated grief, and mental health‐related quality of life were assessed. A multilevel path analysis revealed that loss and trauma significantly impacted on psychological outcomes at both the individual and family levels. Effect sizes ranged from .21 to .68 at the individual level, and .38 to .99 at the family level, highlighting the importance of the family when considering the psychological impact of refugee‐related trauma.
Killing versus witnessing in combat trauma and reports of PTSD symptoms and domestic violence
Authors: Van Winkle Elizabeth P. , Safer Martin A. 
Published: January 2011

AbstractActive participation in combat trauma increased reports of posttraumatic stress disorder (PTSD) symptoms over passive witnessing of trauma. Using archival data from 376 U.S. soldiers who took part in the family interview component of the 1988 National Vietnam Veteran Readjustment Study (NVVRS), findings are that even after statistically accounting for witnessing combat trauma, U.S. soldiers who likely killed enemy soldiers in combat reported elevated levels of PTSD symptoms. Both inference and direct self‐reports were used to measure killing in combat, and both measures accounted equally well for variance in PTSD symptoms. The likelihood of a soldier killing enemy combatants was also weakly related to his spouse's report of physical domestic violence in the past year. Diagnosing the mental health symptoms of combat soldiers should specifically assess whether they actively participated in wounding or killing the enemy.
Cross‐scale monitoring and assessment of land degradation and sustainable land management: A methodological framework for knowledge management
Authors: Reed M. S. , Buenemann M. , Atlhopheng J. , Akhtar‐Schuster M. , Bachmann F. , Bastin G. , Bigas H. , Chanda R. , Dougill A. J. , Essahli W. , Evely A. C. , Fleskens L. , Geeson N. , Glass J. H. , Hessel R. , Holden J. , Ioris A. A. R. , Kruger B. , Liniger H. P. , Mphinyane W. , Nainggolan D. , Perkins J. , Raymond C. M. , Ritsema C. J. , Schwilch G. , Sebego R. , Seely M. , Stringer L. C. , Thomas R. , Twomlow S. , Verzandvoort S. 
Published: January 2011

AbstractFor land degradation monitoring and assessment (M&A) to be accurate and for sustainable land management (SLM) to be effective, it is necessary to incorporate multiple knowledges using a variety of methods and scales, and this must include the (potentially conflicting) perspectives of those who use the land. This paper presents a hybrid methodological framework that builds on approaches developed by UN Food & Agriculture Organisation's land degradation Assessment in Drylands (LADA), the World Conservation Approaches and Technologies (WOCAT) programme and the Dryland Development Paradigm (DDP), and is being applied internationally through the EU‐funded DESIRE project. The framework suggests that M&A should determine the progress of SLM towards meeting sustainability goals, with results continually and iteratively enhancing SLM decisions. The framework is divided into four generic themes: (i) establishing land degradation and SLM context and sustainability goals; (ii) identifying, evaluating and selecting SLM strategies; (iii) selecting land degradation and SLM indicators and (iv) applying SLM options and monitoring land degradation and progress towards sustainability goals. This approach incorporates multiple knowledge sources and types (including land manager perspectives) from local to national and international scales. In doing so, it aims to provide outputs for policy‐makers and land managers that have the potential to enhance the sustainability of land management in drylands, from the field scale to the region, and to national and international levels. The paper draws on operational experience from across the DESIRE project to break the four themes into a series of methodological steps, and provides examples of the range of tools and methods that can be used to operationalise each of these steps. Copyright © 2011 John Wiley & Sons, Ltd.
Polymorphisms in prostate stem cell antigen gene rs2294008 increase gastric cancer risk in Chinese
Authors: Zeng Zhirong , Wu Xiaoqin , Chen Fanggen , Yu Jun , Xue Ling , Hao Yuantao , Wang Yiming , Chen Minhu , Sung Joseph J.Y. , Hu PinJin 
Published: January 2011

AbstractA recent genome‐wide study identified a strong association between polymorphisms in the prostate stem cell antigen (PSCA) gene and the risk of diffuse‐type of gastric cancer in Japanese and Korean population. In this case–control study, we aimed to investigate the possible association between PSCA rs2294008 C/T with clinicopathological features and the prognosis of gastric cancer in a Southern Chinese population. Genotypes of 460 gastric cancer patients and 549 controls were determined by PCR‐restriction fragment length polymorphism (PCR‐RFLP) and DNA sequencing. We found that individuals with at least one copy of the rs2294008T allele (CT or TT genotype) had an increased risk for gastric cancer compared with CC genotype (OR = 1.42, 95% CI = 1.10–1.82, P = 0.006). Further stratification analyses indicated that the effect of PSCA rs2294008T carriers was noteworthy in intestinal type (OR = 1.55, 95% CI = 1.18–2.04, P = 0.002), poorly differentiated (OR = 1.59, 95% CI = 1.19–2.13, P = 0.002), noncardia (OR = 1.55, 95% CI = 1.17–2.04, P = 0.002) subtypes of gastric cancer. Cox proportional hazards analyses demonstrated that TT genotype (HR = 2.12, 95% CI = 1.22–3.69, P = 0.008) as well as TNM staging were prognostic factors of gastric cancer patients. In conclusion, The T allele of PSCA rs2294008 is associated with increased risk of gastric cancer, especially intestinal type, poorly differentiated, early onset, and noncardia gastric cancer in Chinese population. TNM staging and TT genotype might be involved in the prognosis of gastric cancer patients. Mol. Carcinog. © 2011 Wiley‐Liss, Inc.
MDM2 SNP309 contributes to non‐small cell lung cancer survival in Chinese
Authors: Dong Jing , Ren Binhui , Hu Zhibin , Chen Jiaping , Hu Lingmin , Dai Juncheng , Jin Guangfu , Xu Lin , Shen Hongbing 
Published: January 2011

AbstractMurine double minute 2 (MDM2) is a negative regulator of the tumor suppressor gene p53. Single nucleotide polymorphisms in MDM2 and p53 can affect patient's response to chemotherapy as well as overall survival of many cancers. This study aimed to assess the associations between polymorphisms in MDM2 and p53 and survival of non‐small cell lung cancer (NSCLC) patients in Chinese. We selected and genotyped both potentially functional SNPs and tagging SNPs in MDM2 and p53 using Illumina Golden Gate platform in a cohort of 568 NSCLC patients. Associations between genotypes and NSCLC median survival time (MST) were assessed using the Kaplan–Meier method. Cox proportional hazard models were performed with the adjustment for age, stage, smoking, histology, surgical operation, and chemo‐ or radiotherapy status. We found that the MDM2 SNP309 (rs2279744) GT/TT genotypes were associated with a significantly worse survival (MST: 23.0 mo for GT/TT vs. 33.0 mo for GG; log‐rank P = 0.028). In the multivariate Cox regression analyses, the MDM2 SNP309GT/TT genotypes were associated with a 1.42‐fold [HR = 1.42, 95% confidence interval (CI), 1.09–1.84] increased risk of death of NSCLC, compared with SNP309GG genotype. MDM2 SNP309 may be used as one of the candidate biomarkers to predict NSCLC survival. © 2011 Wiley‐Liss, Inc.
The prostaglandin E2 receptor, EP2, regulates survivin expression via an EGFR/STAT3 pathway in UVB‐exposed mouse skin
Authors: Chun Kyung‐Soo , Langenbach Robert 
Published: January 2011

AbstractWe previously reported that cycloogenase (COX)‐2‐generated prostaglandin E2 (PGE2) had anti‐apoptotic effects in UVB‐exposed mouse skin that involved EP2‐mediated signaling (Chun et al., Cancer Res. 2007; 67: 2015). Because survivin is a regulator of cell survival, the possible involvement of COX‐2 and EP2 in survivin expression following UVB exposure of mouse skin was investigated. In wild type mice, UVB exposure time‐dependently increased the levels of survivin and phosphorylated‐signal transducer and activator of transcription 3 (p‐STAT3), a transcription factor that regulates survivin expression; and COX‐2‐ or EP2‐deficiency significantly reduced their induction. Topical application of the COX‐2 inhibitor, celecoxib, also reduced UVB‐induced survivin levels. To further investigate the roles of PGE2 and EP2 in the regulation of survivin, indomethacin was used to inhibit UVB‐induced endogenous PG production. UVB‐induced survivin levels were reduced by indomethacin, and PGE2 and the EP2 agonist, butaprost, partially restored survivin levels. The epidermal growth factor receptor (EGFR) is a downstream effector of EP2 and EGFR inhibition (AG1478) significantly reduced UVB activation of STAT3 and survivin levels. UVB‐induced epidermal apoptosis in COX‐2−/− mice was reduced by butaprost and EGFR inhibition blocked butaprost's protective effects. Furthermore, butaprost in the absence of UVB exposure time‐dependently increased p‐EGFR, p‐STAT3, and survivin levels in naïve mouse skin, whereas the EP4 agonist, PGE1 alcohol, did not significantly increase p‐STAT3 or survivin levels. These data suggest that COX‐2‐generated PGE2 regulates survivin expression in mouse skin, in part, via an EP2‐mediated EGFR/STAT3 pathway. Therefore, targeting the EP2/survivin pathway may provide a strategy for the chemoprevention/chemotherapy of skin cancer. © 2011 Wiley‐Liss, Inc.
Loss of imprinting and abnormal expression of the insulin‐like growth factor 2 gene in gastric cancer
Authors: Zuo Qing‐Song , Yan Ronglin , Feng Dian‐Xu , Zhao Ronghua , Chen Chao , Jiang Yi‐Ming , Cruz‐Correa Marcia , Casson Alan G. , Kang Xiang‐Dong , Han Feng , Chen Teng 
Published: January 2011

AbstractThis study examined the frequency of loss of imprinting (LOI) and expression of the insulin‐like growth factor 2 (IGF2) gene, and their relationship to selected clinical and pathological factors, in a well defined series of 90 Chinese patients with gastric cancer (GC) and 90 matched patients (controls) diagnosed with nonmalignant conditions. Using peripheral blood and gastric tissue samples, polymerase chain reaction‐based assays and restriction endonuclease (Apa I) digestion revealed 33 GC patients and 21 controls to be Apa I informative. LOI of IGF2 was positive in 48.5% (16/33) of primary GC tumor tissues, in 21.2% (7/33) of histologically normal adjacent gastric mucosa (AM) and in 12.1% (4/33) of distant gastric mucosa (DM), and in 15.2% (5/33) of peripheral blood lymphocytes (PBLs). The prevalence of IGF2 LOI in PBL was not statistically different between GC patients (5/33, 15.2%) and control subjects (2/21, 9.5%), P = 0.69. Although patients who were found to have LOI of IGF2 were more likely to have advanced stage gastric tumors (P = 0.04), no statistically significant differences in survival were found based on imprinting status. IGF2 LOI was associated with an increased expression of IGF2 level in both tumors (P < 0.01) and blood (P < 0.01). The results of this study implicate IGF2 LOI in the molecular pathogenesis of GC, most likely through increased IGF2 expression. Although the precise molecular mechanisms by which LOI of IGF2 increases GC risk require further study, LOI of IGF2 may be a potentially important clinical epigenetic marker to identify individuals at increased risk for gastric malignancy. Mol. Carcinog. © 2011 Wiley‐Liss, Inc.
Association of cyclin D1 gene polymorphisms with risk of esophageal squamous cell carcinoma in Kashmir Valley—A high risk area
Authors: Hussain Showket , M. Yuvaraj , Thakur Nisha , Salam Irfana , Singh Neha , Mir Mohammad Muzaffar , Bhat Mohammad Akbar , Siddiqi Mushtaq A. , Das Bhudev C. , Bharadwaj Mausumi 
Published: January 2011

AbstractInvestigation of potential association of SNPs (G870A, rs9344; G1722C, rs678653) of cyclin D1 gene (CCND1) with susceptibility to esophageal squamous cell carcinoma (ESCC) in Kashmir valley (India). The study included 302 subjects comprising 151 ESCC cases and 151 controls. PCR‐RFLP and direct sequencing were employed for genotyping. The G870A polymorphism, the individuals carrying GA + AA genotype was having 2.80‐fold increased risk for development of ESCC (OR 2.8, 95% CI = 1.77–4.4; P = 0.0001) compared to GG genotype. Further a significantly higher risk was observed in individuals who consume >3 cups per day of salted tea (OR = 5.1; 95% CI = 1.6–16.7; P = 0.0016) and had smoking habits (OR = 6.3; 95% CI = 2.9–13.9; P = 0.0005). We also demonstrate for the first time in CCND1 1722 locus, the CC genotype was strongly associated with increased risk of developing ESCC (OR = 2.58; 95% CI = 1.61–4.15; P = 0.0001). In addition, the frequency of polymorphic C allele was also found to be higher in cases (OR = 1.92; 95% CI = 1.37–2.69; P = 0.0002). There appears to be an influence of CCND1 G870A/G1772C genotypes on genetic susceptibility to ESCC. Mol. Carcinog. ©2011 Wiley‐Liss, Inc.
Promoter hypermethylation of fibulin 1 gene is associated with tumor progression in hepatocellular carcinoma
Authors: Kanda Mitsuro , Nomoto Shuji , Okamura Yukiyasu , Hayashi Masamichi , Hishida Mitsuhiro , Fujii Tsutomu , Nishikawa Yoko , Sugimoto Hiroyuki , Takeda Shin , Nakao Akimasa 
Published: January 2011

AbstractHepatocellular carcinoma (HCC) is one of the most common cancers world‐wide but the molecular mechanisms that underlie hepatocarcinogenesis are not fully determined. On the same surgical sample with HCC, we performed microarray‐based gene expression profiling and karyotype analysis using a single nucleotide polymorphism (SNP) array. In addition, quantitative real‐time reverse transcription polymerase chain reaction (PCR), methylation specific PCR (MSP) and immunohistochemical staining were conducted using specimens from 48 patients with HCC. Gene expression profiling showed the expression of fibulin 1 (FBLN1), located on 22q13, to be decreased in tumor tissue. Karyotype analysis revealed no loss of heterozygosity (LOH) since deletions were not detected in 22q, and one of the SNPs on 22q13 showed AB genotype in both cancerous tissue and in corresponding noncancerous tissue, indicating retention of heterozygosity. Quantitative real‐time PCR showed FBLN1 mRNA levels in cancerous tissues to be significantly decreased compared with that in corresponding noncancerous tissues. The immunohistochemical staining results were consistent with both gene expression profiling and quantitative PCR data. Twenty‐four out of 48 HCCs gave a positive result in MSP. Moreover, promoter hypermethylation of FBLN1 was significantly associated with advanced stage HCC, multiple tumors and increased tumor size. Our results indicated that FBLN1 is a novel candidate of tumor suppressor gene and that promoter hypermethylation of FBLN1 is associated with tumor progression in HCC. © 2011 Wiley‐Liss, Inc.