bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of highly informative cross‐species microsatellite panels for the Australian dugong (Dugong dugon) and Florida manatee (Trichechus manatus latirostris) including five novel primers
Authors: HUNTER MARGARET KELLOGG , BRODERICK DAMIEN , OVENDEN JENNIFER R. , TUCKER KIMBERLY PAUSE , BONDE ROBERT K. , MCGUIRE PETER M. , LANYON JANET M. 
Published: September 2009

AbstractThe Australian dugong (Dugong dugon) and Florida manatee (Trichechus manatus latirostris) are threatened species of aquatic mammals in the order Sirenia. Sirenian conservation and management actions would benefit from a more complete understanding of genetic diversity and population structure. Generally, species‐specific microsatellite markers are employed in conservation genetic studies; however, robust markers can be difficult and costly to isolate. To increase the number of available markers, dugong and manatee microsatellite primers were evaluated for cross‐species amplification. Furthermore, one manatee and four dugong novel primers are reported. After polymerase chain reaction optimization, 23 (92%) manatee primers successfully amplified dugong DNA, of which 11 (48%) were polymorphic. Of the 32 dugong primers tested, 27 (84%) yielded product in the manatee, of which 17 (63%) were polymorphic. Dugong and manatee primers were compared and the most informative markers were selected to create robust and informative marker‐panels for each species. These cross‐species microsatellite marker‐panels can be employed to assess other sirenian populations and can provide beneficial information for the protection and management of these unique mammals.
Models for Vortex-Induced Vibration of Cylinders Based on Measured Forces
Authors: Blevins Robert D. 
Published: September 2009

Abstract
This paper develops experimentally based nonlinear models for the vortex shedding forces on oscillating cylinders. The lift in-phase and out-of-phase with cylinder motion and mean drag are determined from experiments with cylinder amplitudes from 0.05 cm to 1.5 cm, and reduced velocities between 2 and 12. The results are reduced to a uniform grid, tabulated, and applied to prediction of resonant, nonresonant, and time history vortex-induced vibration. The results are reduced to a uniform grid, tabulated, and applied to prediction of resonant, nonresonant and time history vortex-induced vibration.
Clinical obligations and public health programmes: healthcare provider reasoning about managing the incidental results of newborn screening
Authors: Miller F A , Hayeems R Z , Bombard Y , Little J , Carroll J C , Wilson B , Allanson J , Paynter M , Bytautas J P , Christensen R , Chakraborty P 
Published: September 2009

Background:Expanded newborn screening generates incidental results, notably carrier results. Yet newborn screening programmes typically restrict parental choice regarding receipt of this non-health serving genetic information. Healthcare providers play a key role in educating families or caring for screened infants and have strong beliefs about the management of incidental results.Methods:To inform policy on disclosure of infant sickle cell disorder (SCD) carrier results, a mixed-methods study of healthcare providers was conducted in Ontario, Canada, to understand attitudes regarding result management using a cross-sectional survey (N  =  1615) and semistructured interviews (N  =  42).Results:Agreement to reasons favouring disclosure of SCD carrier results was high (65.1%–92.7%) and to reasons opposing disclosure was low (4.1%–18.1%). Genetics professionals expressed less support for arguments favouring disclosure (35.3%–78.8%), and more agreement with arguments opposing disclosure (15.7%–51.9%). A slim majority of genetics professionals (51.9%) agreed that a reason to avoid disclosure was the importance of allowing the child to decide to receive results. Qualitatively, there was a perceived “duty” to disclose, that if the clinician possessed the information, the clinician could not withhold it.Discussion:While a majority of respondents perceived a duty to disclose the incidental results of newborn screening, the policy implications of these attitudes are not obvious. In particular, policy must balance descriptive ethics (ie, what providers believe) and normative ethics (ie, what duty-based principles oblige), address dissenting opinion and consider the relevance of moral principles grounded in clinical obligations for public health initiatives.
Differential regulation of H+-ATPases in MDCK-C11 cells by aldosterone and vasopressin
Authors: Dos Santos Priscilla M.C. , Freitas Fabio P. , Mendes Jeane , Tararthuch Ana Lucia , Fernandez Ricardo 
Published: September 2009

The objective of the present work was to characterize the biochemical activity of the proton pumps present in the C11 clone of Madin–Darby canine kidney (MDCK) cells, akin to intercalated cells of the collecting duct, as well as to study their regulation by hormones like aldosterone and vasopressin. MDCK-C11 cells from passages 78 to 86 were utilized. The reaction to determine H+-ATPase activity was started by addition of cell homogenates to tubes contained the assay medium. The inorganic phosphate (Pi) released was determined by a colorimetric method modified from that described by Fiske and Subbarow. Changes in intracellular calcium concentration in the cells was determined using the Ca2+-sensing dye fluo-4 AM. Homogenates of MDCK-C11 cells present a bafilomycin-sensitive activity (vacuolar H+-ATPase), and a vanadate-sensitive activity (H+/K+-ATPase). The bafilomycin-sensitive activity showed a pH optimum of 6.12. ATPase activity was also stimulated in a dose-dependent fashion as K+concentration was increased between 0 and 50 mmol·L–1, with an apparent Kmfor the release of Piof 0.13 mmol·L–1and Vmaxof 22.01 nmol·mg–1·min–1. Incubation of cell monolayers with 10−8 mol·L–1aldosterone for 24 h significantly increased vacuolar H+-ATPase activity, an effect prevented by 10−5 mol·L–1spironolactone. Vacuolar H+-ATPase activity was also stimulated by 10−11 mol·L–1vasopressin, an effect prevented by a V1 receptor-specific antagonist. This dose of vasopressin determined a sustained rise of cytosolic ionized calcium. We conclude that (i) homogenates of MDCK-C11 cells present a bafilomycin-sensitive (H+-ATPase) activity and a vanadate-sensitive (H+/K+-ATPase) activity, and (ii) vacuolar H+-ATPase activity is activated by aldosterone through a genomic pathway and by vasopressin through V1 receptors.
Dietary fenugreek seed regresses preestablished cholesterol gallstones in mice
Authors: Reddy R.L.R. , Srinivasan K. 
Published: September 2009

An animal study was carried out to evaluate the influence of dietary fenugreek seeds on regression of preestablished cholesterol gallstones (CGS). CGS was induced by feeding a high-cholesterol diet for 10 weeks. After CGS induction, the animals were maintained for a further 10 weeks on experimental diets of high cholesterol, 6% fenugreek powder, 12% fenugreek powder, or basal control. Incidence of CGS and its severity were evaluated at the end of this feeding regimen. The incidence of CGS was significantly lowered as a result of dietary fenugreek seeds, the extent of regression being 61% and 64% in the low and high dose groups compared with 10% regression in the basal control group. The antilithogenic influence of dietary fenugreek was accompanied by significant reductions of more than 35% in serum cholesterol concentration. Hepatic cholesterol concentration was also profoundly lowered by dietary fenugreek, being 53%–63% lower than that of the basal control diet. Biliary cholesterol concentration was significantly lower as a result of dietary fenugreek during the post-CGS induction period, resulting in a decreased cholesterol:phospholipid ratio (0.44 and 0.40 compared with 0.79 in the basal control group). Biliary cholesterol : bile acid ratio was lowered by 67% and 73% upon feeding fenugreek, significantly lower than that in the basal control group. The cholesterol saturation index in the bile was also beneficially lowered by fenugreek treatment during the post-CGS induction period (the index was 0.90 and 0.42 compared with 1.86 in the basal control group). The present study provides evidence of the potency of hypolipidemic fenugreek seeds in regressing preestablished CGS, and this beneficial antilithogenic effect is attributable to its primary influence on cholesterol levels. This finding is significant in the context of evolving a dietary strategy to address CGS, which could help in preventing the incidence and regression of existing CGS and controlling possible recurrence.
Regulation of liver glucokinase activity in rats with fructose-induced insulin resistance and impaired glucose and lipid metabolism
Authors: Francini Flavio , Castro María C. , Gagliardino Juan J. , Massa María L. 
Published: September 2009

We evaluated the relative role of different regulatory mechanisms, particularly 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase (PFK2/FBPase-2), in liver glucokinase (GK) activity in intact animals with fructose-induced insulin resistance and impaired glucose and lipid metabolism. We measured blood glucose, triglyceride and insulin concentration, glucose tolerance, liver triglyceride content, GK activity, and GK and PFK2 protein and gene expression in fructose-rich diet (FRD) and control rats. After 3 weeks, FRD rats had significantly higher blood glucose, insulin and triglyceride levels, and liver triglyceride content, insulin resistance, and impaired glucose tolerance. FRD rats also had significantly higher GK activity in the cytosolic fraction (18.3 ± 0.35 vs. 11.27 ± 0.34 mU/mg protein). Differences in GK protein concentration (116% and 100%) were not significant, suggesting a potentially impaired GK translocation in FRD rats. Although GK transcription level was similar, PFK2 gene expression and protein concentration were 4- and 5-fold higher in the cytosolic fraction of FRD animals. PFK2 immunological blockage significantly decreased GK activity in control and FRD rats; in the latter, this blockage decreased GK activity to control levels. Results suggest that increased liver GK activity might participate in the adaptative response to fructose overload to maintain glucose/triglyceride homeostasis in intact animals. Under these conditions, PFK2 increase would be the main enhancer of GK activity.
Food availability in spring influences reproductive output in the seed-preying edible dormouse (Glis glis)
Authors: Kager T. , Fietz J. 
Published: September 2009

Edible dormice ( Glis glis (L., 1766)) display strong annual variation in their reproductive output that is closely related to resource availability, commonly measured through the quantity of seeds produced by their most important food provider, the European beech ( Fagus sylvatica L.). Dormouse mating takes place several weeks before beech seeds ripen, and to the present day it remains unclear how dormice achieve optimized reproductive output in reflection of the quantity of food available in the future. The first aim of this field study carried out over 13 years was thus to investigate the relationship between beech masting and reproductive performance in edible dormice in Germany. If food availability in spring influenced litter size, this would partially explain observed natural variability in offspring numbers. We thus chose an experimental approach and provided supplemental food to edible dormice in the field. Our results showed that numbers and proportions of reproductively active females, as well as litter sizes, between 1993 and 2005 were positively correlated with beech mast. Food supplementation positively affected litter size and litters of food-supplemented females were found to be larger than those of unsupplemented females. Food-supplemented mothers and their offspring gained body mass considerably faster during lactation and were heavier at the end of the lactation period compared with controls. However, juvenile body size, as well as its increase, did not differ between the two treatments. Our results suggest a link between edible dormice reproductive output and food availability after emergence from hibernation.
Intraspecific variation of temperature-induced effects on metamorphosis in the pool frog (Rana lessonae)
Authors: Orizaola Germán , Laurila Anssi 
Published: September 2009

Understanding the factors that affect the process of metamorphosis in species with complex life cycles, and in particular their variation within and among populations, has been rarely explored until recently. We examined the effects of temperature environment on several metamorphic characteristics in three populations of the pool frog ( Rana lessonae Camerano, 1882) by rearing individuals at two temperature environments (20 and 25 °C). Higher temperature shortened the metamorphic period and reduced the absolute mass loss, although there was no difference between the temperatures in the percentage of mass lost. No differences among the populations were detected, but there was significant intrapopulation variation both in the mean and in the plasticity for the duration of metamorphosis. These results indicate that several aspects of metamorphosis are plastic in amphibians, these traits may have considerable intrapopulation variation, and that temperature is a strong factor affecting the process of metamorphosis.
White-winged vampire bats (Diaemus youngi) exchange contact calls
Authors: Carter G. G. , Fenton M. B. , Faure P. A. 
Published: September 2009

Temporally precise vocal exchanges, termed “antiphonal calling”, might allow pair or group members to maintain social contact with greater efficiency than when calling independently. The white-winged vampire bat ( Diaemus youngi (Jentink, 1893)) is a group-living species that produces social calls in antiphonal exchanges. Because white-winged vampire bats can use social calls to discriminate conspecifics, we suspect that one function of these vocal exchanges is to allow group members to know who is where. Here, we tested the prediction that antiphonal calling by groups of white-winged vampire bats increases when the spatial positions of conspecifics change. We recorded social calls from groups of four individually caged bats in total darkness, with each bat located in the corner of a 4 m × 4 m room. During test trials, we shifted the spatial positions of caged bats to new positions. During control trials, caged bats were displaced an equal distance but were returned to their original positions. We found that both the number of social calls and the proportion of antiphonal exchanges were greater during test trials than during control trials. These results suggest that white-winged vampire bats use antiphonal exchanges of social calls to monitor the spatial positions of conspecifics.
Orientation and dispersal of hatchling Blanding’s turtles (Emydoidea blandingii) from experimental nests
Authors: Pappas M. J. , Congdon J. D. , Brecke B. J. , Capps J. D. 
Published: September 2009

We determined initial dispersal directions of 1052 naïve and 278 experienced hatchling Blanding’s turtles (Emydoidea blandingii (Holbrook, 1838)) in experimental arenas in a variety of settings. Dispersal of naïve hatchlings was nonrandom in 7 of 10 sites. All nonrandom dispersal patterns suggested hatchlings primarily used vision to orient toward dark far horizons, particularly those associated with riparian habitats. We found no evidence that hatchlings use positive geotaxis, olfaction, humidity gradients, or scent trailing of other individuals during dispersal. Despite the lack of relationships between the changing position of the sun and relationships between nest sites and wetlands, patterns of dispersal were different for hatchlings released in the morning and late afternoon at two sites. Comparisons of the dispersal of naïve and translocated experienced hatchlings (those with previous exposure to environmental cues) suggest that hatchlings develop a sun compass within 2 days of emergence from nests. Based on all nonrandom dispersals of hatchlings at arenas, the estimated maximum perception distance of hatchlings was 325 m. In some situations, forest succession, agriculture activities, and introduction of pine trees may increase risks faced by hatchlings dispersing from nests by reducing their ability to find wetlands.
Oncogenesis Caused by Loss of the SNF5 Tumor Suppressor Is Dependent on Activity of BRG1, the ATPase of the SWI/SNF Chromatin Remodeling Complex
Authors: Wang Xi , Sansam Courtney G. , Thom Christopher S. , Metzger Daniel , Evans Julia A. , Nguyen Phuong T.L. , Roberts Charles W.M. 
Published: September 2009

Abstract
Alterations in chromatin play an important role in oncogenic transformation, although the underlying mechanisms are often poorly understood. The SWI/SNF complex contributes to epigenetic regulation by using the energy of ATP hydrolysis to remodel chromatin and thus regulate transcription of target genes. SNF5, a core subunit of the SWI/SNF complex, is a potent tumor suppressor that is specifically inactivated in several types of human cancer. However, the mechanism by which SNF5 mutation leads to cancer and the role of SNF5 within the SWI/SNF complex remain largely unknown. It has been hypothesized that oncogenesis in the absence of SNF5 occurs due to a loss of function of the SWI/SNF complex. Here, we show, however, distinct effects for inactivation of Snf5 and the ATPase subunit Brg1 in primary cells. Further, using both human cell lines and mouse models, we show that cancer formation in the absence of SNF5 does not result from SWI/SNF inactivation but rather that oncogenesis is dependent on continued presence of BRG1. Collectively, our results show that cancer formation in the absence of SNF5 is dependent on the activity of the residual BRG1-containing SWI/SNF complex. These findings suggest that, much like the concept of oncogene addiction, targeted inhibition of SWI/SNF ATPase activity may be an effective therapeutic approach for aggressive SNF5-deficient human tumors. [Cancer Res 2009;69(20):8094–101]
Circulating Tumor Cells Are Transcriptionally Similar to the Primary Tumor in a Murine Prostate Model
Authors: Helzer Kimberly T. , Barnes Helen E. , Day Laura , Harvey Jeanne , Billings Paul R. , Forsyth Allyn 
Published: September 2009

Abstract
The abundance of circulating tumor cells (CTC) indicates patient prognosis. Molecular characterization of CTCs may add additional information about a patient's disease. However, currently available methods are limited by contamination with blood cells. We describe a study using a modified CTC-chip to capture CTCs from an orthotopic xenograft model. Using laser capture microscopy to collect CTCs from the chip, we compared transcripts from purified CTCs with those from primary and metastatic tissue. Transcriptional profiles showed strong concordance among primary, metastatic, and CTC sources. Moreover, cells captured on the chip were viable and could be expanded in culture. We conclude that the CTC-chip is a useful tool to further characterize animal models of cancer and that viable CTCs can be isolated and show transcriptional similarity to solid tumors. [Cancer Res 2009;69(19):7860–6]
Dual Phosphoinositide 3-Kinase/Mammalian Target of Rapamycin Blockade Is an Effective Radiosensitizing Strategy for the Treatment of Non–Small Cell Lung Cancer Harboring K-RAS Mutations
Authors: Konstantinidou Georgia , Bey Erik A. , Rabellino Andrea , Schuster Katja , Maira Michael S. , Gazdar Adi F. , Amici Augusto , Boothman David A. , Scaglioni Pier Paolo 
Published: September 2009

Abstract
Non–small cell lung cancer (NSCLC) is a leading cause of cancer-related death worldwide. NSCLC often harbors oncogenic K-RAS mutations that lead to the aberrant activation of several intracellular networks including the phosphoinositide 3-kinase (PI3K)/AKT/mammalian target of rapamycin (mTOR) signaling pathway. Oncogenic K-RAS predicts poor prognosis and resistance to treatment with ionizing radiation (IR). Oncogenic K-Ras expression in the respiratory epithelium is sufficient to initiate NSCLC tumorigenesis, which requires the catalytic subunit of PI3K. Thus, effective inhibition of the PI3K signaling should lead to significant antitumor effects. However, therapy with rapamycin analogues has yielded disappointing results due in part to compensatory up-regulation of AKT. We hypothesized that dual PI3K/mTOR blockade would overcome these limitations. We tested this hypothesis with BEZ235, a novel dual PI3K/mTOR inhibitor that has recently entered clinical development. We found that BEZ235 induces a striking antiproliferative effect both in transgenic mice with oncogenic K-RAS–induced NSCLC and in NSCLC cell lines expressing oncogenic K-RAS. We determined that treatment with BEZ235 was not sufficient to induce apoptosis. However, we found that dual PI3K/mTOR blockade effectively sensitizes NSCLC expressing oncogenic K-RAS to the proapoptotic effects of IR both in vitro and in vivo. We conclude that dual PI3K/mTOR blockade in combination with IR may benefit patients with NSCLC expressing oncogenic K-RAS. These findings may have general applicability in cancer therapy, because aberrant activation of PI3K occurs frequently in human cancer. [Cancer Res 2009;69(19):7644–52]
Induction of Prostatic Intraepithelial Neoplasia and Modulation of Androgen Receptor by ETS Variant 1/ETS-Related Protein 81
Authors: Shin Sook , Kim Tae-Dong , Jin Fang , van Deursen Jan M. , Dehm Scott M. , Tindall Donald J. , Grande Joseph P. , Munz Jan-Marie , Vasmatzis George , Janknecht Ralf 
Published: September 2009

Abstract
ETS variant 1 (ETV1), also known as ETS-related protein 81, is overexpressed in prostate tumors, but whether and how this transcription factor affects tumorigenesis has remained elusive. Here, we show that ETV1 is primarily overexpressed in the most aggressive human prostate tumors. Transgenic ETV1 mice developed prostatic intraepithelial neoplasia as well as hyperplasia/neoplasia in seminal vesicles. Moreover, ETV1 cooperated with the androgen receptor (AR) to bind to the prostate-specific antigen enhancer and stimulate gene transcription. Consistent with its ability to physically interact with AR, ETV1 rendered an ETV1 binding site–driven reporter androgen inducible, and, on the other hand, ETV1 superinduced transcription from an AR binding site on androgen stimulation. In conclusion, our study substantiates that ETV1 overexpression is an underlying cause in the development of prostate and possibly also seminal vesicle cancer. Its interaction with and activation of AR provides a molecular mechanism on how ETV1 exerts its deleterious function. Thus, inhibiting ETV1 or blocking its interaction with AR may represent novel strategies in prostate cancer therapy. [Cancer Res 2009;69(20):8102–10]