bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pre‐ and postnatal diagnosis and outcome of fetuses and neonates with esophageal atresia and tracheoesophageal fistula
Authors: de Jong E. M. , de Haan M. A. M , Gischler S. J. , Hop W. , Cohen‐Overbeek T. E. , Bax N. M. A. , de Klein A. , Tibboel D. , Grijseels E. W. M. 
Published: January 2010

AbstractObjectivesClinical symptoms and ultrasound signs during pregnancy could suggest the presence of esophageal atresia (EA). However, most often EA is diagnosed postnatally. The aim of our study is to evaluate the course and outcome for prenatally and postnatally diagnosed EA. In addition, we studied the outcome of isolated versus nonisolated EA.MethodsIn a retrospective data analysis, ultrasound characteristics, maternal and neonatal variables as well as clinical outcome were compared for fetuses/neonates with prenatal (n = 30) or postnatal (n = 49) diagnosis of EA. Clinical outcome in terms of morbidity and mortality of isolated EA was compared with that of EA complicated by chromosomal or structural anomalies.ResultsPrenatally diagnosed children were born 2 weeks earlier than postnatally diagnosed children (36.4 weeks vs 38.2 weeks; P = 0.02). The former had higher mortality rates (30 vs 12%; P = 0.05) and more associated anomalies (80 vs 59%; P = 0.04). In both subsets, there was a high morbidity rate in the survivors (not significant). Nonisolated EA was associated with greater occurrence of polyhydramnios (53 vs 27%; P = 0.04) and higher mortality rate (28 vs 0%; P = 0.002).ConclusionsMortality was significantly higher in prenatally diagnosed infants and in infants with additional congenital anomalies. Isolated EA is associated with good outcome. Copyright © 2010 John Wiley & Sons, Ltd.
Telehealth as adjunctive therapy in insulin pump treated patients: a pilot study
Authors: Everett J , Kerr D 
Published: February 2010

AbstractNew National Institute for Health and Clinical Excellence guidance is likely to increase the use of insulin pump therapy, and the challenge for diabetes teams is to maintain the initial improvement in HbA1c without extra resources. A telehealth system has been developed where both health professionals and patients can view downloaded pump and blood glucose data.A pilot study in patients with HbA1c >8%, using pump therapy for more than a year, demonstrated a mean reduction from 9.3% to 8.2% at 12 months after using the telehealth system. Patient satisfaction with the system reported more understanding, insight and control by viewing the data, as well as easy access to the health professional. This pilot study has demonstrated that, for some people, using a telehealth approach has resulted in improved diabetes control. Copyright © 2010 John Wiley & Sons.
Discovery of putative oocyte quality markers by comparative ExacTag proteomics
Authors: Powell Michael D. , Manandhar Gaurishankar , Spate Lee , Sutovsky Miriam , Zimmerman Shawn , Sachdev Shrikesh C. , Hannink Mark , Prather Randall S. , Sutovsky Peter 
Published: January 2010

AbstractPurpose: Identification of the biomarkers of oocyte quality, and developmental and reprogramming potential is of importance to assisted reproductive technology in humans and animals.Experimental design: PerkinElmer ExacTag™ Kit was used to label differentially proteins in pig oocyte extracts (oocyte proteome) and pig oocyte‐conditioned in vitro maturation media (oocyte secretome) obtained with high‐ and low‐quality oocytes.Results: We identified 16 major proteins in the oocyte proteome that were expressed differentially in high‐ versus low‐quality oocytes. More abundant proteins in the high‐quality oocyte proteome included kelch‐like ECH‐associated protein 1 (an adaptor for ubiquitin‐ligase CUL3), nuclear export factor CRM1 and ataxia‐telangiectasia mutated protein kinase. Dystrophin (DMD) was more abundant in low‐quality oocytes. In the secretome, we identified 110 proteins, including DMD and cystic fibrosis transmembrane conductance regulator, two proteins implicated in muscular dystrophy and cystic fibrosis, respectively. Monoubiquitin was identified in the low‐quality‐oocyte secretome.Conclusions and clinical implications: A direct, quantitative proteomic analysis of small oocyte protein samples can identify potential markers of oocyte quality without the need for a large amount of total protein. This approach will be applied to discovery of non‐invasive biomarkers of oocyte quality in assisted human reproduction and in large animal embryo transfer programs.
Comparative proteomic analysis of differentially expressed proteins in primary retinoblastoma tumors
Authors: Mallikarjuna Kandalam , Sundaram Curam Sreenivasacharlu , Sharma Yogendra , Deepa Perinkulam Ravi , Khetan Vikas , Gopal Lingam , Biswas Jyotirmay , Sharma Tarun , Krishnakumar Subramanian 
Published: January 2010

AbstractPurpose: To understand the disease mechanism and to identify the potential tumor markers that would help in therapeutics, comparative proteomic analysis of 29 retinoblastoma (RB) tumors was performed using 14 non‐neoplastic retinas (age ranged from 45 to 89 years) as control tissues.Experimental design: 2‐DE and MALDI‐TOF‐TOF MS/MS were used to identify differentially expressed proteins.Results: Twenty‐seven distinct differentially expressed proteins were identified, including 16 upregulated 11 downregulated proteins. Significantly, higher mRNA levels of apolipoprotein A1 (p<0.001), transferrin (TF; p<0.001), CRABP2 (p<0.001), α‐crystallin A (CRYAA; p<0.001) were observed in RBs when compared with normal retinas and hence are consistent with the proteomic data. Immunohistochemistry was also performed for selected proteins on paraffin RB blocks to confirm protein expression. RB with invasion showed significantly higher expression by 2‐DE‐MS/MS analysis of CRABP2 (p<0.001), peroxiredoxin 6 (p=0.025), apolipoprotein A1 (p<0.001), recoverin (p<0.001).Conclusions and clinical relevance: Thus, this study provides a dynamic protein profile of RB tumors, which could provide clues to study the mechanisms of RB oncogenesis and possibly be developed as potential biomarkers for prognosis and therapy.
Revisiting the Warburg effect in cancer cells with proteomics. The emergence of new approaches to diagnosis, prognosis and therapy
Authors: Scatena Roberto , Bottoni Patrizia , Pontoglio Alessandro , Giardina Bruno 
Published: January 2010

AbstractMost cancer cells exhibit elevated levels of glycolysis and this metabolic pathway seems to be related to a greater glucose uptake. This phenomenon, known as the Warburg effect, is considered one of the most fundamental metabolic alterations during malignant transformation. Originally, Warburg hypothesised that the aerobic glycolysis of cancer cells could be just an aspect of a more complex metabolic adaptation. However, this intriguing discovery was partially misinterpreted and disregarded over time. In recent years, the peculiarities of cancer cell metabolism have been re‐evaluated in light of new metabolic data that seem to confirm and to widen the original concept of the Warburg effect. In fact, biochemical, molecular, and, above all, proteomic studies on the multifaceted roles of glycolytic enzymes in cancer cells in general, and in cancer stem cells in particular, seem to suggest more complex functional adaptations. These adaptations result in significantly altered protein expression patterns, and they have fundamental implications for diagnosis, prognosis and therapy. Revisiting the Warburg effect in cancer cells with a proteomic approach could deepen our knowledge of cancer cell metabolism and of cancer cell biology in general. Moreover, by identifying useful diagnostic, prognostic and therapeutic targets, it could significantly impact clinical practice.
Customising an antibody leukocyte capture microarray for systemic lupus erythematosus: Beyond biomarker discovery
Authors: Ho Joshua W. K. , Lin Ming‐Wei , Adelstein Stephen , dos Remedios Cristobal G. 
Published: January 2010

AbstractSystemic lupus erythematosus (SLE) is a complex autoimmune disease that has heterogeneous clinical manifestation with diverse patterns of organ involvement, autoantibody profiles and varying degrees of severity of disease. Research and clinical experience indicate that different subtypes of SLE patients will likely benefit from more tailored treatment regimes, but we currently lack a fast and objective test with high enough sensitivity to enable us to perform such sub‐grouping for clinical use. In this article, we review how proteomic technologies could be used as such an objective test. In particular, we extensively review many leukocyte surface markers that are known to have an association with the pathogenesis of SLE, and we discuss how these markers can be used in the further development of a novel SLE‐specific antibody leukocyte capture microarray. In addition, we review some bioinformatics challenges and current methods for using the data generated by these cell‐capture microarrays in clinical use. In a broader context, we hope our experience in developing a disease specific cell‐capture microarray for clinical application can be a guide to other proteomic practitioners who intend to extend their technologies to develop clinical diagnostic and prognostic tests for complex diseases.
Small gold species supported on alumina. A computational study of α‐Al2O3(0001) and γ‐Al2O3(001) using an embedded‐cluster approach
Authors: Nasluzov Vladimir A. , Shulimovich Tatyana V. , Shor Aleksey M. , Bukhtiyarov Valery I. , Rösch Notker 
Published: January 2010

AbstractWe calculated the structures of and analyzed the bonding in adsorption complexes of small gold species Aun on α‐Al2O3(0001), n = 1–6, and γ‐Al2O3(001), n = 1–5. We applied a scalar‐relativistic gradient‐corrected density functional (DF) method to cluster models of the support that were embedded in an extended elastic polarizable environment (EPE). The shortest AuO distances, 204–211 pm, are consistent with extended X‐ray adsorption fine structure (EXAFS) data for gold clusters on alumina surfaces. The calculated total adsorption energies increase with cluster nuclearity, up to n = 4, but drop for larger adsorbed species. In the gas phase, these small gold clusters exhibit a planar structure which they keep, oriented parallel to the surface, as adsorbates on α‐Al2O3(0001). Unfavorable energy contributions result for larger clusters as their planar shape is notably distorted by the interaction with the support which amounts to 0.5–1.5 eV. On γ‐Al2O3(001), also the larger gold clusters retain their intrinsic planar structure as they adsorb oriented perpendicular to the surface. The corresponding adsorption energies are slightly smaller, 0.3–1.2 eV.
Characterisation and cleaning of oxide support materials for cavity ring‐down spectroscopy
Authors: Kartouzian Aras , Thämer Martin , Heiz Ulrich 
Published: January 2010

AbstractCavity ring‐down spectroscopy (CRDS) has been applied to characterise different oxide materials (amorphous silica, borosilicate and yttria stabilised zirconia YSZ) which are suitable to be used as support material for size‐selected metal clusters. The sensitivity of the spectroscopic method was improved by means of transversal mode matching and spatial filtering of the laser beam profile, reducing the relative error by 50%. The high sensitivity of CRDS allows the detection of trace amounts of impurities and defect sites in the samples, based on their absorption properties. In YSZ, traces of Nd have been detected. The optical quality of the substrates was determined qualitatively according to the measured optical losses. CRDS surface maps have been used to monitor the homogeneity of the support materials, and the influence of ion bombardment on the surface has been studied. It is shown that in the case of BK7® substrates, sputtering with low energy Ar+ ions could remove deposited gold clusters almost completely causing very low damage to the surface. These results were confirmed analytically.
Kinetic Monte Carlo study of self‐organization of low‐dimensional nanostructures on fcc (110) surfaces
Authors: Negulyaev Nikolay N. , Stepanyuk Oleg V. , Niebergall Larissa , Saletsky Alexander M. 
Published: January 2010

AbstractPerforming large‐scale atomic simulations by means of kinetic Monte Carlo method we study room temperature self‐organization of 3d magnetic atoms (Fe, Co) on fcc (110) surfaces (Pd(110), Cu(110)) in the sub‐monolayer regime. The energetics of various diffusion processes relevant for these systems is investigated based on first principles calculations. We reveal that surface‐confined atomic intermixing plays a significant role in the formation of nanostructures. Our results lead to the conclusion that the deposited species (Fe, Co) are captured into the topmost surface layer, while the ad‐layer structure consists mainly of the expelled substrate atoms (Pd, Cu). Our studies shed a light on recent experimental investigations on the metal‐on‐metal growth on fcc (110) surfaces.
Evolution of nanostructure with reaction time for ZnS synthesized by solvothermal process
Authors: Paul Gouri Sankar , Agarwal Pratima 
Published: January 2010

AbstractZnS nanostructures has been successfully synthesized from zinc acetate [(CH3COO)2Zn; 2H2O] and thiourea (NH2CSNH2) by solvothermal process with ethylenediamine (en) solvent at 180 °C for three different reaction times (1 h, 3 h and 5 h) respectively. Various techniques such as XRD, SEM and TEM are employed for structural characterization whereas the composition and chemical purity are determined using EDAX. Photoluminescence studies show broad emission peak around 360 nm corresponding to Zn2+ and S2‐ transitions. In addition, two small sharp peaks originating from vacancy and interstitial states are also observed. Electrical transport studies at room temperature and above predict thermally activated behavior typical of semiconductors (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Rough glass by 3d texture transfer for silicon thin film solar cells
Authors: Zhang Wendi , Bunte Eerke , Worbs Janine , Siekmann Hilde , Kirchhoff Joachim , Gordijn Aad , Hüpkes Jürgen 
Published: January 2010

AbstractTextured glass is prepared by ion beam treatment using wet chemically etched ZnO film as three dimensional etching mask. The shape and rms roughness of the textured glass can be adjusted by changing the initial ZnO thickness, wet‐chemical etching duration and ion beam etching parameters. The maximum rms roughness we achieved is 228 nm, with lateral feature size larger than 2 μm. The glass texturing process allows to study scattering already at the glass/TCO interface to enhance the refractive index step from rough material to silicon. First microcrystalline silicon thin film solar cells grown on the textured glass reveal certain light trapping effect with a short circuit current density up to 20 mA/cm2. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Ultrasharp Si nanowires produced by plasma‐enhanced chemical vapor deposition
Authors: Červenka J. , Ledinský M. , Stuchlíková H. , Stuchlík J. , Výborný Z. , Holovský J. , Hruška K. , Fejfar A. , Kočka J. 
Published: January 2010

AbstractConical silicon nanowires have been grown by gold nanoparticle catalyzed plasma‐enhanced chemical vapor deposition. This method produces Si nanowires with a very fast growth rate (1 μm/min) and unique sharpness (<10 nm). Raman spectroscopy has proved the presence of both crystalline and amorphous Si in the grown Si nanowire layer. The fast growth process of Si nanowires with dimensions below 10 nm holds promises in various applications in electronics, photovoltaics and atomic force microscopy.magnified image(© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Power factor measurements of bismuth telluride nanowires grown by pulsed electrodeposition
Authors: Lee Jongmin , Kim Yunseok , Cagnon Laurent , Gösele Ulrich , Lee Jaeyoung , Nielsch Kornelius 
Published: January 2010

AbstractThe power factors of bismuth telluride nanowires were determined. Large‐scale thermopower measurements of the nanowire arrays were performed under a constant thermal gradient. The local electrical resistance of individual nanowires inside alumina matrix was investigated at ambient conditions by a conductive tip attached to an atomic force microscope (AFM). In order to prevent oxide formation on the nanowire tips, an Au electrode segment was electrochemically deposited onto the nanowires. The optimized structural properties led to a significant improvement of the electrical conductivity of the nanowires from 0.053 to 0.169 × 106 (Ω m)–1 and their Seebeck coefficient also increased moderately from 46.6 μV/K to 55 μV/K. Based on our measurement approach, the determined maximum power factor is 476.3 μW/mK2, which is one of the highest reported values for electrodeposited Bi2Te3 nanowires. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Macroporous Si as an absorber for thin‐film solar cells
Authors: Brendel Rolf , Ernst Marco 
Published: January 2010

AbstractWe separate 15 μm to 32 μm thick macroporous Si films from n‐type crystalline Si wafers by electrochemical etching under back‐side illumination. These layers are surface passivated and have openings in the oxide, for local doping and contacting, over 1% of the cell area. We measure effective lifetimes within a range of 2 to 3 μs. With an analytical model for the effective lifetime in macroporous Si we find an average surface recombination velocity of 75 cm/s, dominantly caused by the contact openings. The measured optical absorption of a 26 μm thick layer allows for a maximum short circuit current density of 37 mA/cm2. The potential efficiency with the current surface passivation quality is 17%. The material loss for separating the macroporous absorber from the wafer is 5 μm. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Electronic properties of two‐dimensional hydrogenated and semihydrogenated hexagonal boron nitride sheets
Authors: Wang Yanli 
Published: January 2010

AbstractUsing first‐principles calculations, we investigate the electronic properties of two‐dimensional hydrogenated and semihydrogenated hexagonal boron nitride (BN) sheets. We find that the hydrogenation effects in BN sheets are quite different from those in the graphene sheets. The hydrogenization changes the band character of BN sheets, which causes the hydrogenated BN sheet having a smaller band gap than the pristine one. While for the semihydrogenated one, the stable B‐semihydro BN sheet is a ferromagnetic metal due to the unpaired 2pz electrons of N atoms. Our studies demonstrate that the electronic properties of BN sheets can be well tuned by the hydrogenation. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Bipolar resistive switching in amorphous titanium oxide thin film
Authors: Jeong Hu Young , Lee Jeong Yong , Ryu Min‐Ki , Choi Sung‐Yool 
Published: January 2010

AbstractUsing isothermal and temperature‐dependent electrical measurements, we investigated the resistive switching mechanism of amorphous titanium oxide thin films deposited by plasma enhanced atomic layer deposition (PEALD) between two aluminum electrodes. We found a bipolar resistive switching (BRS) behavior only in the high temperature region (>140 K) and two activation energies (0.055 eV and 0.13 eV) for the carrier transport in the ohmic current regime. We attribute this discrepancy to the change of the bulk TiO2 Fermi energy level (Ef) induced by the reversible movement of oxygen ions in the vicinity of the Al top electrode region. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Gradient‐doping of a conductive polymer film with a layer‐by‐layer approach
Authors: Kaune Gunar , Müller‐Buschbaum Peter 
Published: January 2010

AbstractBased on a layer‐by‐layer approach a graded doping profile is introduced in a thin film of the conductive polymer poly(N‐vinylcarbazole) (PVK) using 2,4,7‐trinitro‐9‐fluorenone (TNF) as dopant. Three PVK layers with different TNF concentrations are subsequently spin coated on top of each other and due to intermixing at the interfaces a smooth transition between the single layers is achieved. X‐ray reflectivity data of the doped film show a (pp–p–i)‐type profile. With the intrinsic region at the bottom and the highly doped region at the film surface the installed doping profile will have large application potential in organic solar cells.magnified image(© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
CuInSe2 low‐cost thin‐film solar cells made from commercial elemental metallic nanoparticles
Authors: Ungelenk Jan , Haug Veronika , Quintilla Aina , Ahlswede Erik 
Published: January 2010

AbstractWe present a method to prepare low‐cost CuInSe2 (CIS) solar cells using commercially available low‐grade elemental Cu and In nanoparticles without prior synthesis of CIS nanoparticle precursors. A simple non‐vacuum ink deposition method is used where the nanoparticles are dispersed in alcohol and deposited onto a Mo/glass substrate in ambient atmosphere. Depending on the reduction and selenization steps, a compact CIS top layer comprising grains up to a few hundred nano‐ meters in size was observed to have formed using SEM and XRD. Complete solar cells were prepared by conventional methods, leading to a conversion efficiency well above 3%. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Lithium storage in silicon
Authors: Neumann Gerold , Würsig Andreas 
Published: January 2010

AbstractThe Expert Opinion is written by a distinguished scientist and presents his personal view on important and relevant new results of research, highlighting their significance and putting the work into perspective for a broader audience. Please send comments to pss.rapid@wiley‐vch.de or to the author. The text by G. Neumann and A. Würsig refers to the Rapid Research Letter by H. Föll et al., published in this issue of Phys. Status Solidi RRL 4, 4–6 (2010). (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Hydrophilic and morphological aspects of films based on quaternary salts of chitosan for edible applications
Authors: de Britto Douglas , Assis Odilio B.G. 
Published: February 2010

AbstractAiming to improve the homogeneity of films based on chitosan to be applied as edible coating, we carried out some chemical modifications in the polymeric structure by reductive alkylation and quaternization reactions. The resulting derivatives had different hydrophilic behaviours and cast films were characterized by wetting, swelling and topographic measurements. Concerning wetting, all samples presented similar time dependence with receding water contact angles. The quaternized chitosans were the most hydrophilic derivatives, with lower values of contact angles when compared with the parent sample. These derivatives also exhibited a higher swelling tendency. The analysis shows that the relative amount of absorbed water increases according to the length of moiety added. In general, the cast films of chitosan showed good homogeneity, displaying low roughness and regular distribution over a polar surface. On the other hand, alkyl quaternary salts showed an irregular topography with undulation and mountain‐like formation due probably to agglomeration of polymeric chains. The final film morphology was dependent on the polymer solubility, as observed by microscopic techniques. Copyright © 2010 John Wiley & Sons, Ltd.