bluuebunny/crossref_metadata_2025_split huggingface dataset:
Maxillary sinus augmentation via the bone lid technique: A prospective, radiographic case series
Authors: Izzetti Rossana , Cinquini Chiara , Nisi Marco , Baldi Niccolò , Graziani Filippo , Barone Antonio 
Published: April 2024

AbstractBackgroundMaxillary sinus augmentation is one of the most performed procedures to increase the bone quantity of the atrophic maxilla to allow implant placement. The aim of the present case series was to describe a surgical protocol to perform maxillary sinus augmentation with the “bone lid technique,” and its outcomes in a cohort of patients eligible for the procedure.MethodsAfter the initial clinical evaluation, a cone‐beam computed tomography (CBCT) examination was performed for preoperative assessment. Patients were then scheduled for surgical intervention. At 6–9 months follow‐up, patients underwent a second CBCT scan to evaluate bone height following bone graft and to schedule implant placement.ResultsA total of 11 patients were enrolled in the study with a total of 13 sinus lift procedures. Membrane perforation was registered in 4 cases (30.76%). Mean surgical time was 67.69 min (SD 6.51). Postoperative period was uneventful in all patients, in the absence of complications. The mean graft volume increase was 2.46 cm3 (SD 0.85), and the mean height increase was 14.27 mm (SD 3.18). Mean membrane thickness was 1.40 mm (SD 0.75). In all the 4 cases with sinus membrane perforation, the membrane had a thickness lower than 1 mm.ConclusionsThe present study highlights that the maxillary sinus augmentation with bone lid repositioning could provide repeatable results in terms of bone height increase. The technique appears reliable both in terms of bone gain and absence of complications.Key points
The bone lid technique for maxillary sinus augmentation provides repeatable results in terms of bone height increase. The favorable clinical outcomes can be related to an enhancement of bone formation due to the unique osteoconductive and osteoinductive properties of autogenous bone, along with a reduction of soft tissue ingrowth.
Complications were not observed in any of the patients following the surgical procedures.
The risk of Schneiderian membrane perforation is inversely proportional to membrane thickness; the thinner the membrane is, the higher the risk to perforate it.
Correcting mucogingival deformities for pescatarian patients: A clinical case study
Authors: Jung Hyung Jae , Karimbux Nadeem , Dragan Irina F. 
Published: April 2024

ABSTRACTAimThis clinical case study is to highlight the improvement of periodontal health of mandibular canines using a soft tissue alternative of fish origin, a piscine graft.MethodsA 37‐year‐old female patient was referred to a periodontal practice for evaluation of mucogingival deformities around teeth #22 and #27 that were also diagnosed with recession, lack of keratinized tissue (KT), and bilateral high frenum attachment. Multiple soft tissue treatment options were presented to the patient, including autogenous, allograft, or xenograftporcine or piscine.ResultsGiven the patient's dietary preference, piscine option was preferred. The procedures were completed one at a time, first #22 and later #27, using the standard of care procedures for correcting mucogingival deformities using soft tissue alternatives. Post‐surgical visits were scheduled at regular intervals (2, 4, 12, 24, 52 weeks) to evaluate the clinical outcomes. Healing was uneventful and clinical outcomes reveal correction of the mucogingival deformities. The amount of KT at the 52 weeks healing time, measured using an intraoral scanner was 2.12 mm on #22 and 1.78 mm on #27.ConclusionWithin this clinical case's scope, piscine xenograft demonstrates to be a safe and effective soft tissue alternative to correct mucogingival deformities, increasing the KT width and achieving recession coverage. In addition, integration of patient's preference may lead to increased case acceptance and patient compliance.Key pointsWhat new information is this case providing?

The use of a soft tissue alternative of piscine origin that was selected based on the patient's preference to correct bilateral combined mucogingival deformities (recession, lack of KT, and aberrant frenum attachment) around teeth.What is a key step to integrating this soft tissue alternative in clinical practice?

The pre‐hydration of the soft tissue alternative is preferred, compared to other soft tissue alternatives that might not require hydration (xenograft bovine origin).What are the limitations to success in this case?

Confirming with the patient no pre‐existing fish allergies.
Oleanane‐Type Triterpene Glycosides from Camellia phanii and Their α‐Glucosidase Inhibitory Activity
Authors: Lan Hoang Thi Tuyet , Xuan Vu Thi , Anh Bui Thi Mai , Mai Nguyen Thi , Dung Nguyen Viet , Tai Bui Huu , Hang Nguyen Thi Minh , Thao Vu Mai , Nhiem Nguyen Xuan 
Published: April 2024

AbstractThree new oleanane‐type triterpene saponins, named camphanosides A–C (1–3), along with five known compounds, chikusetsusaponin IVa (4), spinasaponin A 28‐O‐glucoside (5), (−)‐epicatechin (6), (−)‐epicatechin 3‐O‐gallate (7), and (−)‐epigallocatechin 3‐O‐gallate (8) were isolated from the leaves Camellia phanii Hakoda & Ninh. Their structures were established by 1D and 2D‐NMR and mass spectral analysis and chemical methods. Moreover, compounds 1–5 were also evaluated for α‐glucosidase inhibitory activity. Compounds 1–3 exhibited moderate α‐glucosidase inhibitory activity with IC50 values of 230.7±18.0, 251.4±22.7, and 421.4±25.6 μM, respectively.
Critical roles of long noncoding RNA H19 in cancer
Authors: Darmadi Darmadi , Chugaeva Uliana Y. , Saleh Raed Obaid , Hjazi Ahmed , Saleem Hiba Muwafaq , Ghildiyal Pallavi , Alwaily Enas R. , Alawadi Ahmed , Alnajar Mohammed Jawad , Ihsan Ali 
Published: April 2024

AbstractLong noncoding RNAs (lncRNAs) are a category of noncoding RNAs characterized by their length, often exceeding 200 nucleotides. There is a growing body of data that indicate the significant involvement of lncRNAs in a wide range of disorders, including cancer. lncRNA H19 was among the initial lncRNAs to be identified and is transcribed from the H19 gene. The H19 lncRNA exhibits significant upregulation in a diverse range of human malignancies, such as breast, colorectal, pancreatic, glioma, and gastric cancer. Moreover, the overexpression of H19 is frequently associated with a worse prognosis among individuals diagnosed with cancer. H19 has been shown to have a role in facilitating several cellular processes, including cell proliferation, invasion, migration, epithelial–mesenchymal transition, metastasis, and apoptosis. This article summarizes the aberrant upregulation of H19 in human malignancies, indicating promising avenues for future investigations on cancer diagnostics and therapeutic interventions.
Mesoporous Pt/N−TiO2/Ti Photocatalytic Plate for Highly Efficient Hydrogen Production
Authors: Hua Zhe , Yang Liu , Li Jing , Wang Junchao , Chen Lixin , Tan Liyuan , Guo Jiaming , Yao Jinlei , Liu Baodan 
Published: April 2024

AbstractMonolithic plate‐type photocatalyst with excellent light absorption, abundant active sites and strong mechanical adhesion is the growing trend of photocatalysis application, as referring to a photovoltaic panel. In this study, we report a large‐scale Pt/N−TiO2/Ti photocatalytic plate with granular and mesoporous structure, which shows a superior photocatalytic performance and adhesion than nanowires and nanosheets photocatalysts. The outstanding photocatalytic performance of Pt/N−TiO2/Ti is attributed to the large exposed surface area, enhanced light absorption, high separation efficiency of photogenerated carriers and excellent surface activity induced by single Pt atom cocatalyst. The best Pt/N−TiO2/Ti sample could exhibit an outstanding photocatalytic hydrogen production activity of 72547.9 μmol ⋅ h−1 ⋅ g−1, and a high apparent quantum efficiency of 47.07 % under a 350 nm light irradiation. It can be anticipated that the proposed method and insights would pave a solid way on the development and application of superior TiO2 based photocatalytic plate.
Self‐Supported Electrocatalyst for Seawater Splitting
Authors: Enkhbayar Enkhtuvshin , Gaur Ashish , Jang Jin Uk , Nayak Arpan Kumar , Na Kyeong‐Han , Choi Won‐Youl , Han HyukSu 
Published: April 2024

AbstractOver the past decades, tremendous effort has been made to enhance the water‐splitting via fabricating eco‐friendly electrocatalyst with increased conductivity, and large number of accessible active sites in lab scale. However, the development of earth abundant efficient electrocatalyst with superior activity for ‐seawater‐splitting remains a great challenge for the researchers. In this regard, self‐supported catalysts are found to be the most promising candidates, they have the features of increased loading, superior adhesion, rapid mass and charge transfer, and easy wettability for large scale hydrogen production via electrochemical seawater splitting. This review investigates different fabrication processes for the self‐supported catalyst, emphasizing their distinct characteristics that contribute to improved activity. Furthermore, we provided a detailed elucidation of the procedure and characteristics of seawater splitting, emphasizing the most recent progress in the creation of self‐supportive catalyst for the hydrogen evolution reaction (HER), oxygen evolution reaction (OER), and bifunctional activity. We have also examined the current barriers and potential prospects for advancing the utilization of self‐supported catalysts in the process of seawater splitting.
Solvent‐Free Mechanosynthesis of Oligopeptides by Coupling Peptide Segments of Different Lengths – Elucidating the Role of Cesium Carbonate in Ball Mill Processes
Authors: Wróblewska Aneta , Bak‐Sypien Irena I. , Paluch Piotr , Wielgus Ewelina , Zając Justyna , Jeziorna Agata , Kaźmierski Sławomir , Potrzebowski Marek J. 
Published: April 2024

AbstractWe report an idea for the synthesis of oligopeptides using a solvent‐free ball milling approach. Our concept is inspired by block play, in which it is possible to construct different objects using segments (blocks) of different sizes and lengths. We prove that by having a library of short peptides and employing the ball mill mechanosynthesis (BMMS) method, peptides can be easily coupled to form different oligopeptides with the desired functional and biological properties. Optimizing the BMMS process we found that the best yields we obtained when TBTU and cesium carbonate were used as reagents. The role of Cs2CO3 in the coupling mechanism was followed on each stage of synthesis by 1H, 13C and 133Cs NMR employing Magic Angle Spinning (MAS) techniques. It was found that cesium carbonate acts not only as a base but is also responsible for the activation of substrates and intermediates. The unique information about the BMMS mechanism is based on the analysis of 2D NMR data. The power of BMMS is proved by the example of different peptide combinations, 2+2, 3+2, 4+2, 5+2 and 4+4. The tetra‐, penta‐, hexa‐, hepta‐ and octapeptides obtained under this project were fully characterized by MS and NMR techniques.
High Pressure Promotes Binding of the Allosteric Inhibitor Zn2+‐Cyclen in Crystals of Activated H‐Ras
Authors: Girard Eric , Lopes Pedro , Spoerner Michael , Dhaussy Anne‐Claire , Prangé Thierry , Kalbitzer Hans Robert , Colloc'h Nathalie 
Published: April 2024

AbstractIn this work, we experimentally investigate the potency of high pressure to drive a protein toward an excited state where an inhibitor targeted for this state can bind. Ras proteins are small GTPases cycling between active GTP‐bound and inactive GDP‐bound states. Various states of GTP‐bound Ras in active conformation coexist in solution, amongst them, state 2 which binds to effectors, and state 1, weakly populated at ambient conditions, which has a low affinity for effectors. Zn2+‐cyclen is an allosteric inhibitor of Ras protein, designed to bind specifically to the state 1. In H‐Ras(wt).Mg2+.GppNHp crystals soaked with Zn2+‐cyclen, no binding could be observed, as expected in the state 2 conformation which is the dominant state at ambient pressure. Interestingly, Zn2+‐cyclen binding is observed at 500 MPa pressure, close to the nucleotide, in Ras protein that is driven by pressure to a state 1 conformer. The unknown binding mode of Zn2+‐cyclen to H‐Ras can thus be fully characterized in atomic details. As a more general conjunction from our study, high pressure x‐ray crystallography turns out to be a powerful method to induce transitions allowing drug binding in proteins that are in low‐populated conformations at ambient conditions, enabling the design of specific inhibitors.
Improving Fatigue Resistance and Autonomous Switching of pH Responsive Hydrazones by Pulses of a Chemical Fuel
Authors: Maity Pankaj , Pradhan Harekrushna , Das Asesh , Dalapati Monotosh , Samanta Dipak 
Published: April 2024

AbstractThe chemically triggered reversible switching of pH‐responsive hydrazones involves rotary motion‐induced configurational changes, serving as a prototype for constructing an array of molecular machines. Typically, the configurational isomerization of such switches into two distinct forms (E/Z) occurs through the alteration of the pH the medium, achieved by successive additions of acid and base stimuli. However, this process results in intermittent operation due to the concomitant accumulation of salt after each cycle, limiting switching performance to only a few cycles (5–6). In this context, we introduce a novel strategy for the autonomous E/Z isomerization of hydrazones in acetonitrile using pulses of trichloroacetic acid as a chemical fuel. The use of this transient acid enabled reversible switching of hydrazones even after 50 cycles without causing significant fatigue. To test the broad viability of the fuel, a series of ortho/para‐substituted hydrazones were synthesized and their switching performance was investigated. The analysis of kinetic data showed a strong dependency of switching operations including the lifetime of transient state, on the electronic properties of substituents. Finally, a distinct color change from yellow to orange due to reversible switching of the para‐methoxy substituted hydrazone was employed for the creation of rewritable messages on commercially available paper.
A Single Terminal [NiII−OH] Catalyst for Direct Julia‐Type Olefination and α‐Alkylation Involving Sulfones and Alcohols
Authors: Pandey Prabhakar K. , Patra Moumita , Ranjan Prabodh , Kumar Pal Nilay , Choudhary Sanjay , Bera Jitendra K. 
Published: April 2024

AbstractA terminal [NiII−OH] complex 1, supported by triflamide‐functionalized NHC ligands, showed divergent reactivity for the reaction of sulfone with alcohol, contingent on base concentration, temperature, and time. Julia‐type olefination of alcohols with sulfones was achieved using one equiv. of base, whereas lowering base loading to 0.5 equiv. afforded α‐alkylated sulfones. Besides excellent substrate scope and selectivity, biologically active stilbene derivatives DMU‐212, pinosylvin, resveratrol, and piceatannol were synthesized in high yield under Julia‐type olefination conditions. An extensive array of controlled experiments and DFT calculations provide valuable insight on the reaction pathway.
High‐Intensity Circular Dichroism of Head‐To‐Tail Regioregular Poly(1,4‐Phenylene)s in the Aggregated State
Authors: Tanaka Rikuya , Yamaoka Seiha , Ikeda Shuichi , Okano Kentaro , Horie Masaki , Minami Hideto , Suzuki Nozomu , Mori Atsunori 
Published: April 2024

AbstractCircular dichroism (CD) studies on poly(1,4‐phenylene)s bearing a chiral side chain in the aggregated conditions were carried out. Little CD was observed in a solution form, while addition of a poor solvent into the polyphenylene solution induced aggregation and a strong CD was observed, accordingly. Applying the controlled degree of polymerization (DP) of poly(1,4‐phenylene) in the use of bidentate diphosphine Chiraphos as a ligand for the nickel catalyst, the relationship of DP with CD strength was studied to reveal to show the highest CD at the DP=84 (gabs=ca. 2×10−2). It was also found that the related aggregation was observed in good solvent 1,2‐dichloroethane upon standing the solution at 4 °C for 3–23 days to observe gabs=ca. 10−1. Studies on the substituent effect of poly(1,4‐phenylene) suggested that CD behaviors were dependent on the type of non‐chiral substituent on the aromatic ring as well as the side‐chain chirality.
Solution‐State Structure of a Long‐Loop G‐Quadruplex Formed Within Promoters of Plasmodium falciparum B var Genes
Authors: Juribašić Kulcsár Marina , Gabelica Valérie , Plavec Janez 
Published: April 2024

AbstractWe report the high‐resolution NMR solution‐state structure of an intramolecular G‐quadruplex with a diagonal loop of ten nucleotides. The G‐quadruplex is formed by a 34‐nt DNA sequence, d[CAG3T2A2G3TATA2CT3AG4T2AG3T2], named UpsB‐Q‐1. This sequence is found within promoters of the var genes of Plasmodium falciparum, which play a key role in malaria pathogenesis and evasion of the immune system. The [3+1]‐hybrid G‐quadruplex formed under physiologically relevant conditions exhibits a unique equilibrium between two structures, both stabilized by base stacking and non‐canonical hydrogen bonding. Unique equilibrium of the two closely related 3D structures originates from a North‐South repuckering of deoxyribose moiety of residue T27 in the lateral loop. Besides the 12 guanines involved in three G‐quartets, most residues in loop regions are involved in interactions at both G‐quartet‐loop interfaces.
Linking of Chemical Reactions and Silica Nanoparticle Contact Using Synthetic Helical Molecules
Authors: Zhang Sheng , Bao Ming , Arisawa Mieko , Yamaguchi Masahiko 
Published: April 2024

Comprehensive SummaryBiological cells exhibit diverse phenomena induced through linking of chemical reactions of molecules and solid surface contact. It is then a significant topic in the field of chemistry to study phenomena induced through this linking using synthetic systems, which can promote our understanding of biological phenomena and can be applied to the development of novel functions. Silica nanoparticles (SNPs), which are synthetic inorganic materials, are attractive for such purposes, because of their following characteristics: they can adsorb large amounts of molecules on their surfaces, they can aggregate through contact between SNPs as well as contact between molecules and SNPs, and the molecules can be easily removed from solutions by precipitation. The contact of SNP surfaces with molecules then affects chemical reactions of molecules and also behaviors of SNPs. This article describes systems derived from synthetic helical molecules and SNPs, which exhibit notable phenomena including selective adsorption and molecular recognition, equilibrium shift, step kinetics with induction period, precipitation with flow and sweeping, and disaggregation and desorption by sonication, in which the high affinity of helical molecules with SNP surfaces plays important roles. Mechanistic models that explain the phenomena are provided. Possible applications are also discussed, including the separation of molecules, capture of intermediates, the storage and release of molecules, equilibrium shift, clocking, and the translation of mechanical stimulations into chemical reactions.
Key Scientists
Prediction of the essential intermolecular contacts for side‐binding of VASP on F‐actin
Authors: Aydin Fikret , Katkar Harshwardhan H. , Morganthaler Alisha , Harker Alyssa J. , Kovar David R. , Voth Gregory A. 
Published: April 2024

AbstractVasodilator‐stimulated phosphoprotein (VASP) family proteins play a crucial role in mediating the actin network architecture in the cytoskeleton. The Ena/VASP homology 2 (EVH2) domain in each of the four identical arms of the tetrameric VASP consists of a loading poly‐Pro region, a G‐actin‐binding domain (GAB), and an F‐actin‐binding domain (FAB). Together, the poly‐Pro, GAB, and FAB domains allow VASP to bind to sides of actin filaments in a bundle, and recruit profilin–G‐actin to processively elongate the filaments. The atomic resolution structure of the ternary complex, consisting of the loading poly‐Pro region and GAB domain of VASP with profilin–actin, has been solved over a decade ago; however, a detailed structure of the FAB‐F‐actin complex has not been resolved to date. Experimental insights, based on homology of the FAB domain with the C region of WASP, have been used to hypothesize that the FAB domain binds to the cleft between subdomains 1 and 3 of F‐actin. Here, in order to develop our understanding of the VASP–actin complex, we first augment known structural information about the GAB domain binding to actin with the missing FAB domain‐actin structure, which we predict using homology modeling and docking simulations. In earlier work, we used mutagenesis and kinetic modeling to study the role of domain‐level binding–unbinding kinetics of Ena/VASP on actin filaments in a bundle, specifically on the side of actin filaments. We further look at the nature of the side‐binding of the FAB domain of VASP at the atomistic level using our predicted structure, and tabulate effective mutation sites on the FAB domain that would disrupt the VASP–actin complex. We test the binding affinity of Ena with mutated FAB domain using total internal reflection fluorescence microscopy experiments. The binding affinity of VASP is affected significantly for the mutant, providing additional support for our predicted structure.
Lysophosphatidic Acid Receptor 1 (LPA1) Antagonists as Potential Migrastatics for Triple Negative Breast Cancer
Authors: Liu Wenjie , Mousa Amr A. K. , Hopkins Austin M. , Wu Yin Fang , Thu Kelsie L. , Campbell Michael , Lees Simon J. , Ramachandran Rithwik , Hou Jinqiang 
Published: April 2024

AbstractMetastasis is responsible for about 90 % of cancer deaths. Anti‐metastatic drugs, termed as migrastatics, offer a distinctive therapeutic approach to address cancer migration and invasion. However, therapeutic exploitation of metastasis‐specific targets remains limited, and the effective prevention and suppression of metastatic cancer continue to be elusive. Lysophosphatidic acid receptor 1 (LPA1) is activated by an endogenous lipid molecule LPA, leading to a diverse array of cellular activities. Previous studies have shown that the LPA/LPA1 axis supports the progression of metastasis for many types of cancer. In this study, we report the synthesis and biological evaluation of fluorine‐containing triazole derivatives as potent LPA1 antagonists, offering potential as migrastatic drugs for triple negative breast cancer (TNBC). In particular, compound 12 f, the most potent and highly selective in this series with an IC50 value of 16.0 nM in the cAMP assay and 18.4 nM in the calcium mobilization assay, inhibited cell survival, migration, and invasion in the TNBC cell line. Interestingly, the compound did not induce apoptosis in TNBC cells and demonstrated no cytotoxic effects. These results highlight the potential of LPA1 as a migrastatic target. Consequently, the LPA1 antagonists developed in this study hold promise as potential migrastatic candidates for TNBC.