bluuebunny/crossref_metadata_2025_split huggingface dataset:
A learning‐based method for optimal dynamic privileged parking permit policy
Authors: Yuan Yun , Li Yitong , Li Xin , Wang Xin 
Published: May 2024

AbstractThe privileged permit service can be provided as an alternative to the conventional meter and reserved services in the off‐street parking lots. In view of the unbalanced demand and the simplistic off‐street parking lot management, this paper proposes a novel parking management problem for setting up and withdrawing the temporary permit‐only policy. To optimize the access rule regarding uncertainty demand on the time of day and the utilization of the parking lot, a deep Q‐learning (DQL) method is proposed to address the uncertainty and dimensionality in the framework of deep reinforcement learning (DRL). To replicate real‐world demand pattern for training deep Q network, a short‐term parking demand model is presented by integrating the long‐short term memory neural network and multivariant Gaussian process. A case study is performed on urban parking lots on university campus. The numerical experiments of a rule‐based strategy, a tabular Q‐learning (TQL) method, and the proposed DQL method are conducted to justify the effectiveness of the proposed method. The proposed method outperforms the static (s, S) inventory policy by 65% and TQL with linear Q‐value estimation by 15% in the total revenue. The sensitivity analyses show the DQL method is capable to handle capacity‐reduced, demand‐increased, and special‐event scenarios while the comparable strategy underperforms the proposed method
Fasciculations detected by muscle ultrasonography in the early stage of amyotrophic lateral sclerosis: An autopsy case
Authors: Moriyoshi Hideyuki , Riku Yuichi , Ito Yasuhiro , Akagi Akio , Sone Jun , Miyahara Hiroaki , Katsuno Masahisa , Yoshida Mari , Iwasaki Yasushi 
Published: May 2024

AbstractWe describe an autopsied case of amyotrophic lateral sclerosis, wherein fasciculations were detected by muscle ultrasonography in the early stage. The myotome areas with fasciculations corresponded to areas of severe pathological lesions. Our results demonstrated that these fasciculations indicate excitability and severity of affected lower motor neurons and significantly contribute to diagnosis.
Thrombectomy and clot analysis‐guided anti‐coagulant/platelet therapy for acute ischemic stroke in a pregnancy complicated with primary antiphospholipid syndrome: A case report
Authors: Nakamura Takashi , Ueno Tatsuya , Haga Rie , Arai Akira , Iwamura Masatoshi , Tomiyama Masahiko 
Published: May 2024

AbstractA 35‐year‐old woman with antiphospholipid syndrome, with 29‐week pregnancy, developed left hemiparesis. Diffusion‐weighted imaging showed a low Alberta Stroke Program Early Computed Tomography (ASPECT) score. The right internal carotid artery occlusion was recanalized by mechanical thrombectomy (MT) and a pathologically white thrombus was confirmed. Clot analysis‐guided anti‐coagulant/platelet therapy was continued, and the patient delivered at 38 weeks and was discharged with a modified Rankin Scale score of 1. We conclude that MT should be considered, even in pregnancy with antiphospholipid syndrome and low ASPECT scores.
Multiomics and biotechnologies for understanding and influencing cadmium accumulation and stress response in plants
Authors: Yu Yan , Alseekh Saleh , Zhu Zonghe , Zhou Kejin , Fernie Alisdair R. 
Published: May 2024

SummaryCadmium (Cd) is one of the most toxic heavy metals faced by plants and, additionally, via the food chain, threatens human health. It is principally dispersed through agro‐ecosystems via anthropogenic activities and geogenic sources. Given its high mobility and persistence, Cd, although not required, can be readily assimilated by plants thereby posing a threat to plant growth and productivity as well as animal and human health. Thus, breeding crop plants in which the edible parts contain low to zero Cd as safe food stuffs and harvesting shoots of high Cd‐containing plants as a route for decontaminating soils are vital strategies to cope with this problem. Recently, multiomics approaches have been employed to considerably enhance our understanding of the mechanisms underlying (i) Cd toxicity, (ii) Cd accumulation, (iii) Cd detoxification and (iv) Cd acquisition tolerance in plants. This information can be deployed in the development of the biotechnological tools for developing plants with modulated Cd tolerance and detoxification to safeguard cellular and genetic integrity as well as to minimize food chain contamination. The aim of this review is to provide a current update about the mechanisms involved in Cd uptake by plants and the recent developments in the area of multiomics approach in terms of Cd stress responses, as well as in the development of Cd tolerant and low Cd accumulating crops.
Come together: Does network management make a difference for collaborative implementation performance in the context of sudden policy growth?
Authors: Hadorn Susanne , Sager Fritz 
Published: May 2024

AbstractCooperative forms of policy implementation bear the promise of being an answer to the policy delivery challenge resulting from policy growth, with the quality of network management often rated as a key success factor. The positive relationship between network management and performance in networks, however, is primarily supported by theoretical reasoning rather than empirical evidence. The present study empirically investigates this relationship in the context of rapid policy growth resulting from a change in the governance structure in the field of smoking prevention in Switzerland. The results of the analyzed 13 Swiss smoking prevention networks and the 187 associated projects show that network management improves the performance of new policy projects by facilitating access to implementing partners and target groups, but has no impact on output delivery in existing interventions. The study shows that networks, if actively managed, can be a means to ensure adequate enforcement in the context of increasing numbers of new policies.
CD59 gene: 143 haplotypes of 22,718 nucleotides length by computational phasing in 113 individuals from different ethnicities
Authors: Srivastava Kshitij , Yin Qinan , Makuria Addisalem Taye , Rios Maria , Gebremedhin Amha , Flegel Willy Albert 
Published: May 2024

AbstractBackgroundCD59 deficiency due to rare germline variants in theCD59gene causes disabilities, ischemic strokes, neuropathy, and hemolysis. CD59 deficiency due to common somatic variants in thePIG‐Agene in hematopoietic stem cells causes paroxysmal nocturnal hemoglobinuria. The ISBT database lists one nonsense and three missense germline variants that are associated with the CD59‐null phenotype. To analyze the genetic diversity of theCD59gene, we determined long‐rangeCD59haplotypes among individuals from different ethnicities.MethodsWe determined a 22.7 kb genomic fragment of theCD59gene in 113 individuals using next‐generation sequencing (NGS), which covered the whole NM_203330.2 mRNA transcript of 7796 base pairs. Samples came from an FDA reference repository and our Ethiopia study cohorts. The raw genotype data were computationally phased into individual haplotype sequences.ResultsNucleotide sequencing of theCD59gene of 226 chromosomes identified 216 positions with single nucleotide variants. Only three haplotypes were observed in homozygous form, which allowed us to assign them unambiguously as experimentally verifiedCD59haplotypes. They were also the most frequent haplotypes among both cohorts. An additional 140 haplotypes were imputed computationally.DiscussionWe provided a large set of haplotypes and proposed three verified long‐rangeCD59reference sequences, based on a population approach, using a generalizable rationale for our choice. Correct long‐range haplotypes are useful as template sequences for allele calling in high‐throughput NGS and precision medicine approaches, thus enhancing the reliability of clinical diagnostics. Long‐range haplotypes can also be used to evaluate the influence of genetic variation on the risk of transfusion reactions or diseases.
A novel transcoronal surgical technique for the treatment of cystic lesions of the distal phalanx: A cadaveric study
Authors: Frietman Sjoerd K. , van Proosdij Rick , Rijkenhuizen Astrid B. M. , van Veggel Elisabeth 
Published: May 2024

AbstractObjectiveTo assess the feasibility of a novel transcoronal approach for the treatment of axial type 3 cysts of the palmar/plantar aspect of the distal phalanx (P3).Study designEx vivo, experimental study.Sample populationTen cadaveric specimens, four forelimbs and six hindlimbs.MethodsAll cadaveric specimens underwent radiographically guided drilling, followed by cortical screw placement. The cartilage was macroscopically assessed after disarticulation of the distal interphalangeal joint. The entry point and trajectory were evaluated with computed tomography. Results were categorized as: axial, near‐axial and abaxial. The minimal distance from the outer margin of the drill hole and the proximal border of the subchondral bone plate was measured using frontal computed tomographic slices.ResultsEight of the 10 screws were in an axial to near‐axial position and nine followed the desired axial to near‐axial trajectory. One screw was inserted axially but continued in an abaxial trajectory. Iatrogenic damage to the joint cartilage, flexor cortex and solar canal of P3 was not observed. All cortical screws were placed close to the proximal subchondral bone‐plate of P3.ConclusionThe desired axial/near‐axial drilling and screw placement, under radiographic guidance, was achieved in nine out of 10 specimens.Clinical significanceThis novel technique seems promising for the treatment of inaccessible P3 cysts. Additional studies are required to investigate its feasibility in clinical cases, and the long‐term outcome following transcystic screw placement of type 3 P3 cysts.
The Peptidoglycan Recognition Protein 1 confers immune evasive properties on pancreatic cancer stem cells
Authors: López-Gil Juan Carlos , García-Silva Susana , Ruiz-Cañas Laura , Navarro Diego , Palencia-Campos Adrián , Giráldez-Trujillo Antonio , Earl Julie , Dorado Jorge , Gómez-López Gonzalo , Monfort-Vengut Ana , Alcalá Sonia , Gaida Matthias M , García-Mulero Sandra , Cabezas-Sáinz Pablo , Batres-Ramos Sandra , Barreto Emma , Sánchez-Tomero Patricia , Vallespinós Mireia , Ambler Leah , Lin Meng-Lay , Aicher Alexandra , García García de Paredes Ana , de la Pinta Carolina , Sanjuanbenito Alfonso , Ruz-Caracuel Ignacio , Rodríguez-Garrote Mercedes , Guerra Carmen , Carrato Alfredo , de Cárcer Guillermo , Sánchez Laura , Nombela-Arrieta César , Espinet Elisa , Sanchez-Arevalo Lobo Víctor Javier , Heeschen Christopher , Sainz Bruno 
Published: May 2024

Objective
Pancreatic ductal adenocarcinoma (PDAC) has limited therapeutic options, particularly with immune checkpoint inhibitors. Highly chemoresistant ‘stem-like’ cells, known as cancer stem cells (CSCs), are implicated in PDAC aggressiveness. Thus, comprehending how this subset of cells evades the immune system is crucial for advancing novel therapies.

Design

We used the KPC mouse model (

LSL-Kras
G12D/+
; LSL-Trp53
R172H/+
; Pdx-1-Cre

) and primary tumour cell lines to investigate putative CSC populations. Transcriptomic analyses were conducted to pinpoint new genes involved in immune evasion. Overexpressing and knockout cell lines were established with lentiviral vectors. Subsequent
in vitro
coculture assays,
in vivo
mouse and zebrafish tumorigenesis studies, and
in silico
database approaches were performed.

Results
Using the KPC mouse model, we functionally confirmed a population of cells marked by EpCAM, Sca-1 and CD133 as authentic CSCs and investigated their transcriptional profile. Immune evasion signatures/genes, notably the gene peptidoglycan recognition protein 1 (PGLYRP1), were significantly overexpressed in these CSCs. Modulating PGLYRP1 impacted CSC immune evasion, affecting their resistance to macrophage-mediated and T-cell-mediated killing and their tumourigenesis in immunocompetent mice. Mechanistically, tumour necrosis factor alpha (TNFα)-regulated PGLYRP1 expression interferes with the immune tumour microenvironment (TME) landscape, promoting myeloid cell-derived immunosuppression and activated T-cell death. Importantly, these findings were not only replicated in human models, but clinically, secreted PGLYRP1 levels were significantly elevated in patients with PDAC.

Conclusions
This study establishes PGLYRP1 as a novel CSC-associated marker crucial for immune evasion, particularly against macrophage phagocytosis and T-cell killing, presenting it as a promising target for PDAC immunotherapy.
Targeting MYC effector functions in pancreatic cancer by inhibiting the ATPase RUVBL1/2
Authors: Vogt Markus , Dudvarski Stankovic Nevenka , Cruz Garcia Yiliam , Hofstetter Julia , Schneider Katharina , Kuybu Filiz , Hauck Theresa , Adhikari Bikash , Hamann Anton , Rocca Yamila , Grysczyk Lara , Martin Benedikt , Gebhardt-Wolf Anneli , Wiegering Armin , Diefenbacher Markus , Gasteiger Georg , Knapp Stefan , Saur Dieter , Eilers Martin , Rosenfeldt Mathias , Erhard Florian , Vos Seychelle M , Wolf Elmar 
Published: May 2024

Objective
The hallmark oncogene MYC drives the progression of most tumours, but direct inhibition of MYC by a small-molecule drug has not reached clinical testing. MYC is a transcription factor that depends on several binding partners to function. We therefore explored the possibility of targeting MYC via its interactome in pancreatic ductal adenocarcinoma (PDAC).

Design
To identify the most suitable targets among all MYC binding partners, we constructed a targeted shRNA library and performed screens in cultured PDAC cells and tumours in mice.

Results
Unexpectedly, many MYC binding partners were found to be important for cultured PDAC cells but dispensable in vivo. However, some were also essential for tumours in their natural environment and, among these, the ATPases RUVBL1 and RUVBL2 ranked first. Degradation of RUVBL1 by the auxin-degron system led to the arrest of cultured PDAC cells but not untransformed cells and to complete tumour regression in mice, which was preceded by immune cell infiltration. Mechanistically, RUVBL1 was required for MYC to establish oncogenic and immunoevasive gene expression identifying the RUVBL1/2 complex as a druggable vulnerability in MYC-driven cancer.

Conclusion
One implication of our study is that PDAC cell dependencies are strongly influenced by the environment, so genetic screens should be performed in vitro and in vivo. Moreover, the auxin-degron system can be applied in a PDAC model, allowing target validation in living mice. Finally, by revealing the nuclear functions of the RUVBL1/2 complex, our study presents a pharmaceutical strategy to render pancreatic cancers potentially susceptible to immunotherapy.
Developmental sources of crash risk in young drivers
Authors: Arnett J J 
Published: May 2024

Objective:To outline various sources of crash risk among young drivers that are developmental (age based) factors.Methods and Results:First, a distinction is made between adolescence (ages 10–18) and emerging adulthood (ages 18–25) in order to shed light on the reasons for especially high crash rates among 16–17 year old drivers relative to 18–25 year olds. Then various developmental sources of risk in adolescence are described, including the power of friends, the optimistic bias, and adolescent emotionality. The reasons for especially high crash rates among young males are discussed, with an emphasis on how American ideas about manhood promote driving risks. Finally, a cross national comparison between adolescents in the United States and Denmark shows how developmental risks interact with driving policies.Conclusions:The high crash rates of adolescents relative to emerging adults and of emerging adults relative to older drivers can be explained in part by developmental factors.
Receptiveness to monkeypox vaccines and public health communication strategies among gay, bisexual and other men who have sex with men in Singapore: cross-sectional quantitative and qualitative insights
Authors: Chan Zach Yung Shen , Chong Shao Yuan , Niaupari Steph , Harrison-Quintana Jack , Lim Jue Tao , Dickens Borame , Kularathne Yudara , Wong Chen Seong , Tan Rayner Kay Jin 
Published: May 2024

ObjectivesMonkeypox (mpox) was declared as a global health emergency by the WHO, with most reported cases disproportionately involving gay, bisexual and other men who have sex with men (GBMSM). This study explored the willingness of Singaporean GBMSM to receive mpox vaccines and engage in changes to sexual behaviour, and analysed the factors influencing both decisions.MethodsAn online cross-sectional study was disseminated through community groups and Grindr from September to October 2022 among GBMSM in Singapore, where we surveyed respondents’ demographics, stigma associated with mpox, perceived risks of sexually transmitted infections (STIs) and changes to sexual behaviours in response to mpox. Descriptive statistics and multivariable linear and logistics regression analyses, as well as thematic analysis of data, were also conducted.Results237 GBMSM community members responded to the survey, with the majority being receptive to vaccine and 67.5% indicating changes to sexual behaviour in view of rising mpox infections. Predictors of vaccine receptivity among GBMSM included self-perceived mpox risk (adjusted OR (aOR)=0.44, 95% CI 0.07, 0.82) and self-perceived STI risk (aOR=0.39, 95% CI 0.03, 0.76). Predictors for changes to sexual behaviour included self-perceived mpox stigma (aOR=1.17, 95% CI 1.08, 1.27), self-perceived mpox risk (aOR=1.22, 95% CI 1.03,1.44), age (aOR=0.96, 95% CI 0.93, 1.00) and race (aOR=0.31, 95% CI 0.10, 0.93). In the thematic analysis, respondents raised concerns about vaccine effectiveness, side effects, cost and privacy.ConclusionsOur findings suggest that the rise in mpox infections have prompted changes to GBMSM’s sexual practices. In general, GBMSM are willing to receive the mpox vaccine but are concerned about the physical and social consequences of uptake. These concerns should be addressed when vaccines are released.
Revascularisation patterns and characteristics after erythropoietin pretreatment and multiple burr holes in patients who had acute stroke with perfusion impairment
Authors: Lee Seong-Joon , Park So Young , Park Geun Hwa , Lee Jin Soo , Lim Yong Cheol , Hong Ji Man 
Published: May 2024

BackgroundTransdural collaterals, originating mainly from the extracalvarial superficial temporal artery and intracalvarial middle meningeal artery via the external carotid artery (ECA), have been observed after revascularisation surgery. However, the origin of these collaterals in patients with stroke with perfusion insufficiency is not yet known. Therefore, we studied the revascularisation patterns and characteristics based on the origin of these collaterals.MethodsWe employed erythropoietin pretreatment and performed multiple burr holes under local anaesthesia to achieve transdural revascularisation in patients with acute stroke with perfusion insufficiency. After 6 months, we reassessed the transfemoral cerebral angiography to evaluate the revascularisation patterns. The collaterals were categorised into intracalvarial ECA-dominant (originating from the middle meningeal artery), extracalvarial ECA-dominant (originating from the superficial temporal or occipital artery) and balanced groups. We compared various imaging parameters among these groups.ResultsOverall, 87 patients with 103 treated hemispheres were involved. Among them, 57.3% were classified as intracalvarial ECA-dominant, 20.4% as extracalvarial ECA-dominant and 22.3% as balanced. Most of the hemispheres with intracalvarial or extracalvarial collaterals (vs balanced collaterals) showed successful revascularisation (78/80 (97.5%) vs 12/23 (52.1%)), p<0.001). In ultrasonographic haemodynamic changes according to revascularisation pattern, only the intracalvarial ECA-dominant revascularisation was significantly associated with specific changes in ECA blood flow, leading to the conversion to a low-resistance ECA Doppler sonography waveform.ConclusionsOur findings suggest that intracalvarial ECA-dominant revascularisation plays a crucial role in the formation of transdural collaterals following combined therapy. These distinct changes in ECA haemodynamics can be non-invasively identified through bedside ultrasound studies.
Up Close: Asian University for Women
Authors: Ahmad Kamal 
Published: May 2024

Abstract
At a time when equalizing measures like affirmative action are being challenged, a women's university is uplifting the most neglected and defenseless populations in Bangladesh. Yet the Asian University for Women (AUW) faces additional challenges in providing excellent higher education. This essay discusses how AUW also confronts mounting pernicious state control of education by transforming state-university relationships, and how, despite resurfacing nationalism and parochialism, it advocates for regional collaboration in its student body. In a conflict-ridden world, it marshals political, financial, and diplomatic prowess to provide a liberating pathway to those marooned in conflict. While fostering equality through its undergraduate and graduate programs, AUW has raised an age-old question concerning a university's function in an unjust, violence-prone, and divided world. Its answer: the best institutions embrace disadvantaged members of society through education aimed at emancipating those under the yoke of oppression.
Global Education without Walls: A Multidisciplinary Investigation of University Learning in Online Environments across Disciplines
Authors: Zlatkin-Troitschanskaia Olga 
Published: May 2024

Abstract
Increasingly, students use the internet for self-directed learning in higher education, requiring them to develop skills to determine which information is reliable and accurate. Although the need to understand, evaluate, and promote such skills is crucial, little is known about the students’ search for and use of online sources (and the key influences of those sources) in higher education. Current research indicates both that students need specific skills to successfully engage and learn with online materials, and that university practitioners need to rethink their curricula and instructional approaches for online teaching in the age of ChatGPT and other AI-supported tools. Interdisciplinary theoretical and empirical methods can help us gain a deeper understanding of how students develop the various skills required for successful online learning, and how we can support them across domains.