bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of exercise and environmental complexity on deficits in trace and contextual fear conditioning produced by neonatal alcohol exposure in rats
Authors: Schreiber W.B. , St. Cyr S.A. , Jablonski S.A. , Hunt P.S. , Klintsova A.Y. , Stanton M.E. 
Published: May 2012

AbstractIn rodents, voluntary exercise and environmental complexity increases hippocampal neurogenesis and reverses spatial learning and long‐term potentiation deficits in animals prenatally exposed to alcohol. The present experiment extended these findings to neonatal alcohol exposure and to delay, trace, and contextual fear conditioning. Rats were administered either 5.25 g/kg/day alcohol via gastric intubation or received sham‐intubations (SI) between Postnatal Day (PD) 4 and 9 followed by either free access to a running wheel on PD 30–41 and housing in a complex environment on PD 42–72 (wheel‐running plus environmental complexity; WREC) or conventional social housing (SHSH) from PD 30 to 72. Adult rats (PD 80 ± 5) received 5 trials/day of a 10‐s flashing‐light conditioned stimulus (CS) paired with .8 mA footshock either immediately (delay conditioning) or after a 10‐s trace interval (trace conditioning) for 2 days. Neonatal alcohol exposure impaired context and trace conditioning, but not short‐delay conditioning. The WREC intervention did not reverse these deficits, despite increasing context‐related freezing in ethanol‐exposed and SI animals. © 2012 Wiley Periodicals, Inc. Dev Psychobiol 55: 483–495, 2013
Gold Nanoparticles Modified Electrode for the Determination of an Antihypertensive Drug
Authors: Atta Nada F. , Galal Ahmed , Azab Shereen M. 
Published: May 2012

AbstractThe nature of binding between Terazosin (TR) and gold nanoparticles (Au‐Nps) is investigated using UV‐vis and fluorescence spectroscopies, cyclic voltammetry, SEM, and EIS. The results suggest that Au‐Nps are effective carriers for TR. An electrochemical sensor for TR is introduced using Au‐Nps electrodeposited on carbon paste electrode. The effect of parameters including pH and scan rate on the response was investigated. A linear range from 8.0×10−9 to 5.4×10−5 mol L−1 with correlation coefficient of 0.9995 and detection limit of 1.2×10−10 mol L−1 was obtained. This sensor was used for determining TR spiked in urine, and excellent recovery results are achieved.
Towards the development of molecular markers for apple volatiles
Authors: Ulrich Detlef , Dunemann Frank 
Published: May 2012

ABSTRACTImproving flavour quality of apple varieties is an important but complex breeding goal. Flavour is among the key factors of apple fruit quality but in spite of the analytical and biochemical knowledge about volatiles little is known about the genetic and molecular basis of apple aroma. The aim of this paper is to give an overview about the complex analytical investigations on metabolite patterns, quantitative trait locus mapping and development of a first functional marker for acetate esters with high sensory impact based on single nucleotide polymorphisms within a candidate gene encoding a key enzyme involved in the last step of ester biosynthesis (alcohol acyl‐transferase, MdAAT1). Copyright © 2012 John Wiley & Sons, Ltd.
GABAA receptor modulation by the volatile fractions of Sideritis species used as ‘Greek’ or ‘Turkish’ mountain tea
Authors: Kessler Artur , Villmann Carmen , Sahin‐Nadeem Hilal , Pischetsrieder Monika , Buettner Andrea 
Published: May 2012

ABSTRACTSideritis spp. is a member of the Labiateae family, used in traditional folk medicine and as a calming tea preparation. Dichloromethane extracts of the aerial parts of four Sideritis species were prepared, and the volatile fractions were separated via solvent‐assisted flavour evaporation distillation. In vitro electrophysiological techniques were used to investigate the physiological effects of these aroma extracts on ionotropic γ‐aminobutyric acid receptors (GABAA) in comparison to extracts of Lavandula spp. (lavender) obtained by the same approach. The plant extracts of Sideritis spp. and Lavandula spp. increased the maximal current responses gated by the agonist GABA, both in whole cell patch clamp recordings as well as in two electrode voltage clamp assays. Thereby, the volatile fractions caused a dose‐dependent enhancement of GABAergic currents. Differences in activity between the various species were probably due to variations in odorant composition, either on a qualitative or quantitative basis. Thus, the plant material contains volatile organic compounds, which are able to modulate a GABA‐mediated response and thereby possibly contribute to a sedative effect in vivo. Copyright © 2012 John Wiley & Sons, Ltd.
Aloe‐emodin inhibits adipocyte differentiation and maturation during in vitro human mesenchymal stem cell adipogenesis
Authors: Subash‐Babu Pandurangan , Alshatwi Ali A. 
Published: May 2012

AbstractIn this study, we examined the effects of Aloe‐emodin (AE) on the inhibition of adipocyte differentiation during 3‐isobutyl‐1‐methylxanthine (IBMX)‐induced adipocyte differentiation in human mesenchymal stem cells (hMSCs). AE treatment (5, 10, and 20 µM) of preadipocyte cells resulted in a significant (p < 0.05) decrease in glycerol phosphate dehydrogenase and triglyceride levels as well as an increase in lactate dehydrogenase activity and attenuated lipid accumulation compared with untreated differentiated adipocytes. Using quantitative reverse transcription polymerase chain reaction, we studied the mRNA expression levels of resistin, adiponectin, aP2, lipoprotein lipase, PPARγ, and tumor necrosis factor‐α in hMSCs undergoing adipocyte differentiation; treatment with AE decreased the expression of these adipogenic genes and decreased adipocyte differentiation. In addition, AE suppresses the differentiation of hMSCs into adipocytes by downregulating PPARγ and C/EBPα expressions. AE significantly inhibited hMSCs proliferation and preadipocyte differentiation within the first 2 days of treatment, indicating that the antiadipogenic effect. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:291–300, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21415
On Lusin's area integrals and g‐functions in certain Dunkl and Laguerre settings
Authors: Szarek Tomasz Z. 
Published: May 2012

AbstractWe investigate $g$‐functions and Lusin's area type integrals related to certain multi‐dimensional Dunkl and Laguerre settings. We prove that the considered square functions are bounded on the weighted Lp, 1<p<∞, and from L1 into the weak L1.
On some bilinear problems on weighted Hardy‐Sobolev spaces
Authors: Cascante C. , Ortega J. M. 
Published: May 2012

AbstractIn this paper we study the bilinear problem of characterizing the positive Borel measures μ on Sn, satisfying

where \documentclass{article}\usepackage{amssymb}\begin{document}\pagestyle{empty}$H_s^2(w)$\end{document} and \documentclass{article}\usepackage{amssymb}\begin{document}\pagestyle{empty}$H_t^2(w)$\end{document} are weighted Hardy‐Sobolev spaces, under adequate conditions on the weight w.
MicroRNA profiling of carcinogen‐induced rat colon tumors and the influence of dietary spinach
Authors: Parasramka Mansi A. , Dashwood W. Mohaiza , Wang Rong , Abdelli Amir , Bailey George S. , Williams David E. , Ho Emily , Dashwood Roderick H. 
Published: May 2012

ScopeMicroRNA (miRNA) profiles are altered in chronic conditions such as cardiovascular disease, diabetes, neurological disorders, and cancer. A systems biology approach was used to examine, for the first time, miRNAs altered in rat colon tumors induced by 2‐amino‐1‐methyl‐6‐phenylimidazo[4,5‐b]pyridine (PhIP), a heterocyclic amine carcinogen from cooked meat.Methods and resultsAmong the most highly dysregulated miRNAs were those belonging to the let‐7 family. Subsequent computational modeling and target validation identified c‐Myc and miRNA‐binding proteins Lin28A/Lin28B (Lin28) as key players, along with Sox2, Nanog, and Oct‐3/4. These targets of altered miRNAs in colon cancers have been implicated in tumor recurrence and reduced patient survival, in addition to their role as pluripotency factors. In parallel with these findings, the tumor‐suppressive effects of dietary spinach given postinitiation correlated with elevated levels of let‐7 family members and partial normalization of c‐myc, Sox2, Nanog, Oct‐3/4, HmgA2, Dnmt3b, and P53 expression.ConclusionWe conclude that the let‐7/c‐Myc/Lin28 axis is dysregulated in heterocyclic amine‐induced colon carcinogenesis, and that the tumor suppressive effects of dietary spinach are associated with partial normalization of this pathway.
Feasibility and benefits of a school‐based academic and counseling program for older school‐age students with asthma
Authors: Kintner Eileen , Cook Gwendolyn , Allen April , Meeder Linda , Bumpus Janell , Lewis Kimberly 
Published: May 2012

AbstractAn asthma education program for older school‐age students with asthma was developed in collaboration with school personnel, healthcare professionals, and community partners. Feasibility and benefits were evaluated using a single‐group, prospective, quasi‐experimental design. The convenience sample, consisting of 28 grade 6 and 7 students with asthma, had 50% males, a diverse racial background, and a wide range of incomes. Feasibility and benefits were demonstrated by comparing pre‐intervention to 1‐ and 12‐month post‐intervention evaluation of students who completed more than 70% of the 10 sessions. Statistically significant improvements in cognitive, behavioral, psychosocial, and quality of life outcomes were seen from pre‐intervention to 1‐month post‐intervention (all p < .03). Severity of asthma significantly improved from 1‐ to 12‐month post‐intervention (p = .04). © 2012 Wiley Periodicals, Inc. Res Nurs Health 35:507–517, 2012
Risk factors associated with the need for a tracheostomy in extremely low birth weight infants
Authors: Viswanathan Sreekanth , Mathew Ajith , Worth Anne , Mhanna Maroun J. 
Published: May 2012

AbstractIn an attempt to determine the risk factors associated with the need for a tracheostomy in extremely low birth weight (ELBW) infants, a retrospective, case control study was conducted (each infant with a tracheostomy [case] was matched to two controls). Medical records were reviewed for patients' characteristics, risk factors for tracheostomy and outcome. During the study period (June 1996 to Dec 2010), 934 ELBW infants were admitted to our institution and nine infants had a tracheostomy and were matched to 18 controls. There were no differences in birth weight (BW) and gestation age (GA) between cases and controls (828.1 ± 136.2 g vs. 822.0 ± 140.9 g [P = 0.91] and 26.6 ± 1.8 weeks vs. 26.5 ± 1.6 weeks [P = 0.88], respectively). In comparison to their controls, infants with a tracheostomy had a higher rate of intubation (median 13 [11–15] vs. 3 [2–5], P ≤ 0.001), a higher rate of total intubation attempts (median 18 [13–21] vs. 5.5 [3–7], P = 0.001), and more days of mechanical ventilation prior to their tracheostomy (mean 100.7 ± 27.7 vs. 29.2 ± 19.8 days [P < 0.001]). Also infants with a tracheostomy had a higher rate of non‐congenital upper airway obstruction (55% [5/9] vs. 0% [0/18]; P = 0.001), a higher rate of chronic lung disease (100% [9/9] vs. 5% [1/18]; P < 0.001) and a higher mortality (44% [4/9] vs. 0% [0/18]; P = 0.007) than their controls. In conclusion, chronic lung disease, multiple intubations and intubation attempts, duration of mechanical ventilation, and non‐congenital upper airway obstruction are risk factors associated with tracheostomies in ELBW infants. Pediatr Pulmonol. 2013; 48:146–150. © 2012 Wiley Periodicals, Inc.
Fludarabine nucleoside induces accumulations of p53, p63 and p73 in the nuclei of human B‐lymphoid cell lines, with cytosolic and mitochondrial increases in p53
Authors: Almazi Juhura G. , Mactier Swetlana , Best O. Giles , Crossett Ben , Mulligan Stephen P. , Christopherson Richard I. 
Published: May 2012

PurposeHuman Raji cells treated with fludarabine nucleoside (2‐FaraA, 3 μM) undergo apoptosis with accumulation of p53 in the nuclei as multiple phosphorylated isoforms and C‐terminal truncated derivatives. Changes induced by 2‐FaraA in the levels of p53, p63 and p73 in the nuclear, cytosolic and mitochondrial fractions have been determined in four human B‐lymphoid cell lines that are TP53‐functional (Raji and IM9) and TP53‐mutated (MEC1 and U266).Experimental designThe B‐lymphoid cell lines were treated with 2‐FaraA (3 μM, 24 h, 48 h) and viability determined. Protein extracts of subcellular fractions from 2‐FaraA‐treated cells were analysed by 1D and 2D electrophoresis; multiple phosphorylated isoforms and truncated derivatives were identified by Western blots for p53, p63 and p73.Resultsp53 and p63 were present in all three fractions, while p73 was only detected in nuclei. After treatment with 2‐FaraA, nuclear p53, p63 and p73 accumulated as multiple phosphorylated isoforms and truncated derivatives. The association of p63 with mitochondria in human cells is novel.Conclusions and clinical relevanceComprehensive information on the subcellular distributions and responses of p53, p63 and p73 to 2‐FaraA provides additional insight into mechanisms for induction of apoptosis in the treatment of B‐lymphoproliferative disorders with fludarabine.
Strain‐induced ripples in graphene nanoribbons with clamped edges
Authors: Baimova Julia A. , Dmitriev Sergey V. , Zhou Kun 
Published: May 2012

AbstractMolecular dynamics is employed to study the mechanical behavior of graphene nanoribbons with clamped edges under in‐plane strain. Buckling of nanoribbons results in the appearance of periodic ripples whose orientation, wavelength, and amplitude can be controlled by varying strain components and nanoribbon width. This study shows a way of controlling physical properties of nanoribbons by introducing strain‐induced ripples. magnified imageExample of ripples in strained graphene nanoribbon with clamped edges.
A resampling approach for interval‐valued data regression
Authors: Ahn Jeongyoun , Peng Muliang , Park Cheolwoo , Jeon Yongho 
Published: May 2012

AbstractWe consider interval‐valued data that frequently appear with advanced technologies in current data collection processes. Interval‐valued data refer to the data that are observed as ranges instead of single values. In the last decade, several approaches to the regression analysis of interval‐valued data have been introduced, but little work has been done on relevant statistical inferences concerning the regression model. In this paper, we propose a new approach to fit a linear regression model to interval‐valued data using a resampling idea. A key advantage is that it enables one to make inferences on the model such as the overall model significance test and individual coefficient test. We demonstrate the proposed approach using simulated and real data examples, and also compare its performance with those of existing methods. © 2012 Wiley Periodicals, Inc. Statistical Analysis and Data Mining, 2012
Mechanism of Cellular Uptake of Graphene Oxide Studied by Surface‐Enhanced Raman Spectroscopy
Authors: Huang Jie , Zong Cheng , Shen He , Liu Min , Chen Biao , Ren Bin , Zhang Zhijun 
Published: May 2012

AbstractThe last few years have witnessed rapid development of biological and medical applications of graphene oxide (GO), such as drug/gene delivery, biosensing, and bioimaging. However, little is known about the cellular uptake mechanism and pathway of GO. In this work, surface‐enhanced Raman scattering (SERS) spectroscopy is employed to investigate the cellular internalization of GO loaded with Au nanoparticles (NPs) by Ca Ski cells. The presence of Au NPs on the surface of GO enables detection of enhanced intrinsic Raman signals of GO inside the cell. The SERS results reveal that GO is distributed inhomogeneously inside the cell. Furthermore, internalization of Au‐GO into Ca Ski cells is mainly via clathrin‐mediated endocytosis, and is an energy‐dependent process.
Rational Design and Nanofabrication of Gecko‐Inspired Fibrillar Adhesives
Authors: Hu Shihao , Xia Zhenhai 
Published: May 2012

AbstractGecko feet integrate many intriguing functions such as strong adhesion, easy detachment, and self‐cleaning. Mimicking gecko toe pad structure leads to the development of new types of fibrillar adhesives useful for various applications. In this Concept article, in addition to the design of adhesive mimics by replicating gecko geometric features, we show a new trend of rational design by adding other physical, chemical, and biological principles on to the geometric merits, for enhancing robustness, responsive control, and durability. Current challenges and future directions are highlighted in the design and nanofabrication of biomimetic fibrillar adhesives.
Fluorine‐Free Mould Powders for Billet Casting – Technological Parameters and Industrial Tests
Authors: Klug Jeferson L. , Silva Daniel R. , Freitas Suzana L. , Pereira Márcia M. S. M. , Heck Nestor C. , Vilela Antônio C. F. , Jung Detlef 
Published: May 2012

AbstractThere is a clear trend in industry to eliminate fluorine from metallurgical slags. Regarding the continuous casting process, fluorine in mould powders is undesirable due to the following reasons: (i) evolves easily from slags producing health‐injurious gaseous substances like hydrofluoric acid, (ii) creates problems for storage and utilization of solid waste, and (iii) causes machinery corrosion. A collaboration among universities, mould powder supplier, and steel producer was established, aiming for the development of a new F‐free mould powder for billet casting. Firstly, technological parameters for a particular composition were determined: melting characteristics, viscosity, melting behavior, and crystallization tendency. Afterwards, industrial trials were performed in a Brazilian steelworks, doing some evaluations during the continuous casting process, such as ΔT between water cooling inlet and outlet, slag pool thickness, melting performance, powder consumption, Al2O3 pick‐up, and effect on SEN erosion. Comparable surface quality for the billets was obtained when using the F‐free mould powder, since the amount of scrap produced during the rolling process was not critical.
Occupancy dynamics of breeding crawfish frogs in southeastern Indiana
Authors: Williams Perry J. , Robb Joseph R. , Karns Daryl R. 
Published: May 2012

AbstractWe studied the occupancy dynamics of breeding crawfish frogs (Lithobates areolatus) at 45 randomly selected grasslands across 208 km2 at Big Oaks National Wildlife Refuge, in southeastern Indiana, USA, during March 2010 and 2011. We developed a suite of hypotheses explaining the relationship between occupancy, detection, and environmental covariates. We fit our hypotheses using multiseason occupancy models, and compared them in an information‐theoretic, model‐selection framework. Our top model suggested that the detection probability had a positive, linear relationship with time, temperature, and the amount of rain 24 hours before the survey, and had a quadratic relationship with date, which peaked on 19 March. Our top model supported our hypothesis that occupancy probability was positively correlated with grassland size; larger grasslands were more likely to be occupied by crawfish frogs. Based on our results, we recommend that managers conserve large tracts of grasslands near breeding sites. We recommend that in southeastern Indiana crawfish frog‐breeding surveys be conducted in mid‐ to late‐March, and that each call point be surveyed for 15 minutes. We provide a model to increase the precision of detection probability estimates for call surveys that target calling crawfish frogs. Published 2012. This article is a U.S. Government work and is in the public domain in the USA.
Creatine metabolism in urea cycle defects
Authors: Boenzi Sara , Pastore Anna , Martinelli Diego , Goffredo Bianca Maria , Boiani Arianna , Rizzo Cristiano , Dionisi‐Vici Carlo 
Published: May 2012

AbstractCreatine (Cr) and phosphocreatine play an essential role in energy storage and transmission. Maintenance of creatine pool is provided by the diet and by de novo synthesis, which utilizes arginine, glycine and s‐adenosylmethionine as substrates. Three primary Cr deficiencies exists: arginine:glycine amidinotransferase deficiency, guanidinoacetate methyltransferase deficiency and the defect of Cr transporter SLC6A8. Secondary Cr deficiency is characteristic of ornithine‐aminotransferase deficiency, whereas non‐uniform Cr abnormalities have anecdotally been reported in patients with urea cycle defects (UCDs), a disease category related to arginine metabolism in which Cr must be acquired by de novo synthesis because of low dietary intake. To evaluate the relationships between ureagenesis and Cr synthesis, we systematically measured plasma Cr in a large series of UCD patients (i.e., OTC, ASS, ASL deficiencies, HHH syndrome and lysinuric protein intolerance). Plasma Cr concentrations in UCDs followed two different trends: patients with OTC and ASS deficiencies and HHH syndrome presented a significant Cr decrease, whereas in ASL deficiency and lysinuric protein intolerance Cr levels were significantly increased (23.5 vs. 82.6 μmol/L; p < 0.0001). This trend distribution appears to be regulated upon cellular arginine availability, highlighting its crucial role for both ureagenesis and Cr synthesis. Although decreased Cr contributes to the neurological symptoms in primary Cr deficiencies, still remains to be explored if an altered Cr metabolism may participate to CNS dysfunction also in patients with UCDs. Since arginine in most UCDs becomes a semi‐essential aminoacid, measuring plasma Cr concentrations might be of help to optimize the dose of arginine substitution.
Functional and electrophysiological characterization of four non‐truncating mutations responsible for creatine transporter (SLC6A8) deficiency syndrome
Authors: Valayannopoulos Vassili , Bakouh Naziha , Mazzuca Michel , Nonnenmacher Luc , Hubert Laurence , Makaci Fatna‐Léa , Chabli Allel , Salomons Gajja S. , Mellot‐Draznieks Caroline , Brulé Emilie , de Lonlay Pascale , Toulhoat Hervé , Munnich Arnold , Planelles Gabrielle , de Keyzer Yves 
Published: May 2012

AbstractIntellectual disability coupled with epilepsy are clinical hallmarks of the creatine (Cr) transporter deficiency syndrome resulting from mutations in the SLC6A8 gene. So far characterization of pathogenic mutations of SLC6A8 has been limited to Cr uptake. The aim of our study was to characterize the electrogenic and pharmacological properties of non truncating SLC6A8 mutations identified in patients presenting variable clinical severity. Electrophysiological and pharmacological properties of four mutants (including two novel ones) were studied in X. laevis oocyte expression system. Creatine uptake was assessed with [14C]‐Cr in X. laevis and patients’ fibroblasts. Subcellular localization was determined by immunofluorescence and western blot. All mutants were properly targeted to the plasma membrane in both systems. Mutations led to the complete loss of both electrogenic and transport activities in X. laevis and Cr uptake in patients’ fibroblasts. Among the Cr analogs tested, guanidinopropionate induced an electrogenic activity with the normal SLC6A8 transporter similar to creatine whereas a phosphocreatine derivative, PCr‐Mg‐CPLX, resulted in partial activity. SLC6A8 mutants displayed no electrogenic activity with all Cr analogs tested in X. laevis oocytes. Although the mutations altered various domains of SLC6A8 Cr uptake and electrogenic properties were completely inhibited and could not be dissociated. Besides the metabolic functions of Cr, the loss of SLC6A8 electrogenic activity, demonstrated here for the first time, may also play a role in the altered brain functions of the patients.