bluuebunny/crossref_metadata_2025_split huggingface dataset:
Correspondence between hysteroscopic and histologic findings in women with chronic endometritis
Authors: CICINELLI ETTORE , TINELLI RAFFAELE , LEPERA ACHIROPITA , PINTO VINCENZO , FUCCI MARIAROSA , RESTA LEONARDO 
Published: July 2010

AbstractObjective. To estimate if findings in women with chronic endometritis (CE) at fluid hysteroscopy correlate with the severity of inflammation at histology and to assess the possibility to score the severity of inflammation by fluid hysteroscopy. Design. Controlled clinical study. Setting. University hospital. Population. A total of 211 outpatient women with a hysteroscopic diagnosis of CE and 30 control women without any such signs. Methods. All patients underwent endometrial biopsy by means of a 3‐mm Novak's curette connected to a 20‐ml syringe without any anesthesia. Main outcome measures. Hysteroscopic records were reviewed and compared with histologic findings by using an arbitrary inflammation score (Grades 0–2, no inflammation to severe infiltration, nodules and glands disruption, respectively). Results. A significant correlation between focal hyperemia and isolated micropolyps with Grade 1 inflammation and between generalized hyperemia, presence of diffuse micropolyps and polypoid endometrium with Grade 2 inflammation was found. Hysteroscopic and histologic grading showed good agreement (kappa index = 0.62). Conclusions. In women affected by CE the severity of histological alterations may be reliably evaluated by fluid hysteroscopy. This information may be clinically useful not only for giving a prognosis and as a basis for interpretation of patients' complaints, but also for monitoring treatment.
Health-Care Staff Attitudes Towards Self-Harm Patients
Authors: Gibb Sheree J. , Beautrais Annette L. , Surgenor Lois J. 
Published: July 2010

Objective: To examine attitudes towards self-harm patients and need for training about self-harm amongst health-care staff in Christchurch, New Zealand. Methods: Health-care staff from a general and a psychiatric hospital completed a questionnaire about their attitudes towards self-harm patients and their need for training about self-harm. Results: A total of 195 staff members completed the questionnaire (response rate 64.4%). Overall, health-care staff had both positive and negative attitudes towards self-harm patients. Staff believed that their contact was helpful to self-harm patients, that they were patient and understanding, and were optimistic about patients’ outcomes. However, staff did not feel confident working with self-harm patients and believed that their training in this area was inadequate. Attitudes were not significantly associated with age, gender, or experience. However, more negative attitudes were significantly associated with higher levels of burnout (through high emotional exhaustion (p <0.0002) and low personal accomplishment (p <0.003)). Staff comments indicated that their greatest difficulties working with self-harm patients included repetitive self-harm, frustrating and difficult patient behaviour, communication difficulties, and time pressure. Staff suggestions for improvement included more training, provision of a handbook or guidelines, and greater flexibility with patient allocations. Conclusions: Overall, health-care staff had positive attitudes towards self-harm patients, and a strong desire to help such patients. However, staff did not feel confident working with self-harm patients and had a strong desire for additional training in this area. Additional staff training in working with self-harm patients could have the potential to increase staff confidence and attitudes and enhance patient care.
Help-Seeking for Substance Use, Anxiety and Affective Disorders Among Young People: Results from the 2007 Australian National Survey of Mental Health and Wellbeing
Authors: Reavley Nicola J. , Cvetkovski Stefan , Jorm Anthony F. , Lubman Dan I. 
Published: July 2010

Objective: To provide a more detailed analysis of the relationship between type and severity of mental disorders (substance use, anxiety and affective disorders) and help-seeking in those aged 16–24 years compared to those aged 25–44 and 45–85 years. Method: Data from the National Survey of Mental Health and Wellbeing (NSMHWB) which was conducted in 2007. The survey sample comprised residents of private dwellings across Australia aged 16–85 years. Results: More than one in four Australians aged 16–24 years experienced a mental disorder in the preceding 12 months. This compared to one in five in those aged 16–85 years. Fewer than one in four 16–24-year-olds with a 12-month mental disorder accessed health services in a 12-month period compared with just over one in three of those aged 16–85. The gap in help-seeking was primarily related to higher rates of substance use disorders and low help-seeking associated with these, particularly in young men. Conclusions: Findings from the 2007 NSMHWB show that the gap in help-seeking in young people with mental health problems is largely due to high rates of substance use disorders and the low rates of help-seeking associated with these. In order to address this gap there is a need for better coordination and integration of mental health and alcohol and drug services within primary care settings. Population health approaches that tackle erroneous beliefs about alcohol and related harms and improve overall mental health literacy are also needed.
Cognitive Behavioural Group Treatment for Chinese Parents with Children with Developmental Disabilities in Melbourne, Australia: An Efficacy Study
Authors: Wong Fu Keung Daniel , Poon Ada 
Published: July 2010

Objectives: This study attempted to test the efficacy of a culturally attuned cognitive behavioural therapy (CBT) group for Chinese parents with children with developmental disabilities at risk of developing mental health problems in Melbourne, Australia. It was hypothesized that the participants in the experimental group would have less parenting stress and fewer dysfunctional attitudes, rules, and values, and better mental health and quality of life than the participants in the control group post-test. Method: A total of 58 participants were randomly assigned into CBT and waiting list control groups. While ANCOVAs were used to compare the differences in General Health Questionnaires-12 (GHQ-12), Parenting Stress Index- Parent Domain (PSI-PD), Quality of Life Enjoyment and Satisfaction Questionnnaire-18 (Q-LES-Q-18) and Dysfunctional Attitude Scale (DAS) between participants of the experiemental and control groups, effect size statistics were performed to measure the magnitude of changes in the above instruments at post treatment. Results: After ten weeks of treatment, the participants in the CBT group showed significant improvement in GHQ-12, Parenting Stress Index (PSI)-Parent Domain and Q-LES-Q-18 scores, but not in DAS scores. The effect size statistics revealed large differences in GHQ-12, PSI-Parent Domain and Q-LES-Q-18 scores between the participants in the experimental and control groups at post-treatment. When a GHQ score of 4 or greater was used as the recommended cut-off score, about 89% and 10% of the participants in the experimental and control groups, respectively, were classified as not at-risk cases at post-treatment. Conclusion: The initial findings suggest that a culturally attuned CBT group may help Chinese parents with children with developmental disabilities to reduce their parenting stress and improve their general mental health and quality of life.
IL-3 Inhibits Human Osteoclastogenesis and Bone Resorption through Downregulation of c-Fms and Diverts the Cells to Dendritic Cell Lineage
Authors: Gupta Navita , Barhanpurkar Amruta P. , Tomar Geetanjali B. , Srivastava Rupesh K. , Kour Supinder , Pote Satish T. , Mishra Gyan C. , Wani Mohan R. 
Published: July 2010

Abstract
IL-3 is an important cytokine that regulates hematopoiesis and functions as a link between the immune and the hematopoietic system. In this study, we investigated the role and mechanism of IL-3 action on human osteoclast formation and bone resorption using PBMCs. PBMCs differentiate into functional osteoclasts in the presence of M-CSF and receptor activator of NF-κB ligand as evaluated by 23c6 expression and bone resorption. We found that IL-3 dose-dependently inhibited formation of 23c6-positive osteoclasts, bone resorption and C-terminal telopeptide of type I collagen, a collagen degradation product. The inhibitory effect of IL-3 on bone resorption was irreversible. To investigate the mechanism of IL-3 action, we analyzed the effect of IL-3 on the receptor activator of NF-κB and c-Fms receptors and c-Fos, PU.1, NFAT cytoplasmic 1, and RelB transcription factors essential for osteoclastogenesis. IL-3 significantly inhibited c-Fms and downregulated both PU.1 and c-Fos at both mRNA and protein level. Furthermore, IL-3–treated cells showed increased expression of dendritic cell markers CD1a and CD80 and decreased expression of monocyte/macrophage marker CD14. Interestingly, IL-3 inhibited formation of human osteoclasts derived from blood monocytes and bone marrow cells of osteoporotic individuals. Thus, IL-3 may have therapeutic potential as an antiosteolytic agent in treatment of osteoporosis.
Pan–CC Chemokine Neutralization Restricts Splenocyte Egress and Reduces Inflammation in a Model of Arthritis
Authors: Buatois Vanessa , Fagète Séverine , Magistrelli Giovanni , Chatel Laurence , Fischer Nicolas , Kosco-Vilbois Marie H. , Ferlin Walter G. 
Published: July 2010

Abstract
Chemokines are key regulators of leukocyte trafficking and play a crucial role under homeostatic and inflammatory conditions. Because chemokines are involved in multiple pathologies, they represent an attractive class of therapeutic targets. However, because of the redundancy of this system, neutralizing a single chemokine may be insufficient to achieve therapeutic benefit. Our strategy was to use a Fc-fusion recombinant protein form of the poxvirus-derived viral CC chemokine inhibitor protein (vCCI-Fc) that has the ability to specifically bind to multiple CC chemokines and neutralize their activity. In this study, we demonstrate first that, in vivo, vCCI-Fc prevents CC chemokine-dependent migration of macrophages into inflamed tissue of carageenan-challenged mice. We next studied this effect of inhibiting CC chemokine activity in a model more relevant to human disease, collagen-induced arthritis. Mice receiving vCCI-Fc revealed a striking retention of splenocytes, including activated and IFN-γ–secreting CD4+ and CD8+ T cells, that was associated with a concomitant decrease of cells in the draining lymph nodes. These phenomena resulted in a significant decrease in the incidence of disease and a reduction in clinical score, joint inflammation, and cartilage destruction as compared with mice receiving isotype control. Taken together, these results define a role for CC chemokines in the control of disease, as interfering with their function leads to a previously unappreciated role of controlling inflammatory cell trafficking in and out of secondary lymphoid organs.
Type 1 Diabetes in BioBreeding Rats Is Critically Linked to an Imbalance between Th17 and Regulatory T Cells and an Altered TCR Repertoire
Authors: van den Brandt Jens , Fischer Henrike J. , Walter Lutz , Hünig Thomas , Klöting Ingrid , Reichardt Holger M. 
Published: July 2010

Abstract
Diabetes-prone BioBreeding (DP-BB) rats spontaneously develop type 1 diabetes mellitus (T1DM) on grounds of their MHC haplotype RT1u and a point mutation in the Gimap5 gene. In this study, we report that DP-BB rats exhibit an increasingly severe imbalance, in particular between Th17 and regulatory T (Treg) cells, within the first months of age. This can be assigned to an excess in effector T cells because neither the percentage nor the function of the Treg cells is compromised. Flow cytometric analysis of Vβ segment usage and CDR3 spectratyping further suggest that the disturbed repertoire of peripheral T cells may also contribute to the development of T1DM in DP-BB rats. Importantly, expansion of Treg cells in vivo by means of a CD28 superagonistic Ab as well as adoptive transfer of Treg cells efficiently interferes with the development of T1DM in DP-BB rats, whereas treatment with conventional Th cells does not afford protection. Using a newly generated strain of enhanced GFP transgenic rats, we could further demonstrate that the transferred Treg cells persist in the recipient rats for several months and partially correct the imbalance between Th17 and Treg cells. Thus, our data support the hypothesis that unchecked effector T cell action and a disturbed T cell repertoire contribute to the development of T1DM in DP-BB rats, which may also have implications for a better understanding of the human disease.
Dendritic Cells Continue To Capture and Present Antigens after Maturation In Vivo
Authors: Drutman Scott B. , Trombetta E. Sergio 
Published: July 2010

Abstract
Dendritic cell (DC) maturation is critical for the regulation of T cell responses. The downregulation of endocytosis on maturation is considered a key adaptation that dissociates prior Ag capture by DCs from subsequent T cell engagement. To study the dynamics of Ag capture and presentation in situ, we studied the capacity for Ag uptake by DCs matured in their natural tissue environment. We found that after maturation in vivo, mouse DCs retained a robust capacity to capture soluble Ags. Furthermore, Ags internalized by mature DCs were efficiently presented on MHC class II and cross-presented on MHC class I. These results suggest that under inflammatory conditions, mature DCs may contribute to T cell stimulation without exclusively relying on prior exposure to Ags as immature DC precursors.
Graded Attenuation of TCR Signaling Elicits Distinct Autoimmune Diseases by Altering Thymic T Cell Selection and Regulatory T Cell Function
Authors: Tanaka Satoshi , Maeda Shinji , Hashimoto Motomu , Fujimori Chihiro , Ito Yoshinaga , Teradaira Shin , Hirota Keiji , Yoshitomi Hiroyuki , Katakai Tomoya , Shimizu Akira , Nomura Takashi , Sakaguchi Noriko , Sakaguchi Shimon 
Published: July 2010

Abstract
Mice with a mutation of the ζ-associated protein of 70 kDa gene (skg mutation) are genetically prone to develop autoimmune arthritis, depending on the environment. In a set of mice with the mutation, the amount of ζ-associated protein of 70 kDa protein as well as its tyrosine phosphorylation upon TCR stimulation decreased from +/+, skg/+, skg/skg, to skg/− mice in a stepwise manner. The reduction resulted in graded alterations of thymic positive and negative selection of self-reactive T cells and Foxp3+ natural regulatory T cells (Tregs) and their respective functions. Consequently, skg/− mice spontaneously developed autoimmune arthritis even in a microbially clean environment, whereas skg/skg mice required stimulation through innate immunity for disease manifestation. After Treg depletion, organ-specific autoimmune diseases, especially autoimmune gastritis, predominantly developed in +/+, at a lesser incidence in skg/+, but not in skg/skg BALB/c mice, which suffered from other autoimmune diseases, especially autoimmune arthritis. In correlation with this change, gastritis-mediating TCR transgenic T cells were positively selected in +/+, less in skg/+, but not in skg/skg BALB/c mice. Similarly, on the genetic background of diabetes-prone NOD mice, diabetes spontaneously developed in +/+, at a lesser incidence in skg/+, but not in skg/skg mice, which instead succumbed to arthritis. Thus, the graded attenuation of TCR signaling alters the repertoire and the function of autoimmune T cells and natural Tregs in a progressive manner. It also changes the dependency of disease development on environmental stimuli. These findings collectively provide a model of how genetic anomaly of T cell signaling contributes to the development of autoimmune disease.
New methodologies for biofilms control in food industry
Authors: Čapla Jozef , Zajác Peter , Vietoris Vladimí­r , Bajzí­k Pavol 
Published: July 2010

The complete removal of biofilms on food equipment surfaces is essential to ensure food safety and quality. However, cells in biofilms exhibit greater resistance against the action of sanitizers and other antimicrobial agents compared to their free living counterparts, making them much more difficult to remove. They can be a significant source of post - processing contamination and could potentially harbor pathogens in food processing platns. The biotechnology sector is just beginning to tackle the problem of biofilms by developing antimicrobial agents with novel mechanisms of action. Some studies seek to prevent biofilm formation, others aim to develop antimicrobial agents to treat existing biofilms, and still others are trying to disrupt the polymeric ties that bind the biofilms together.
The consumption of acidophylus drinks and yogurts in selected population of pupils in years 2004 and 2008
Authors: Habánová Marta , Lorková Marta , Kopčeková Jana 
Published: July 2010

The aim of this paper was to compare the frequency of consumption and the amount of consumed acidophylus drinks and yogurts at 427 respondents from various schools at the age between 12 and 26 years in two monitored years. 223 males and 204 females participaded at the survey. The questionaire again confirmed low level of yogurts and acidophylus drinks consumption. The consumption of white yogurts was confirmed only by 2 % of questioned pupils. Fruit yogurts are more popular among pupils and it is necessary to say that we registred the increase of fruit yogurts consumption in year 2008, mainly in cream fruit yogurts. The daily consumption of these increased with male population from 14.8 % in year 2004 to 26.2 % in year 2008. The significant increase of consumtption occured also with female population of half-fat yogurts and the similar trend was also niticed in low-fat fruit yogurts, mainly within the female population. The consumption of clabber, acidophylus drinks and buttermilk, according to our survey in year 2008 went up in comparison with year 2004 in male population by 8.3 % and with female population by 9.5 %. Based on the monitored frequency of consumption a few times per week we detected growing trend in yogurt and acidophylus beverages, which we evaluate as the positive sing.
Quality of hemp seed oil depending on its obtaining
Authors: Poustkova Ivana , Babička Luboš , Kouřimská Lenka , Siegrová Gabriela , Staruch Ladislav 
Published: July 2010

Hemp (Cannabis sativa L.) is probably one of the oldest field crops used in nutrition, but also for the production of fibres for clothes, ropes or canvas. Cannabis sativa is one of the most spread species of cannabis which belongs to family Cannabinaceae. The seeds are important part of cannabis sativa, which contains high part of lipids and proteins. It provides also valuable essential fatty acids, carbohydrates, fiber, vitamins and minerals. Due to low content of THC is possible to produce valuable oil from seeds, which is used in cosmetic and food industry.
The aim of this work was to evaluate composition of hemp seeds from one harvest, observe and compare quality of parameters both cold pressed hemp seed oil and hemp seed oil by CO2 extraction.
Both oils are comparable in composition of fatty acids which follow from results of analyses. Also contents of sterols and moisture are similar in both oils. The saponification value is similar in both oils, conformable to as a iodine value. Also were found dissimilarities in colours, phospholipides, unsaponifiable matter, acid value and peroxide value. The cold pressed hemp seed oil contained lower values of unsaponifiable matter, colours and higher concentration of phospholipides and lower acid value. It is caused by influence of CO2. The oxidation stability of cold pressed hemp seed oil was four times higher than oil by CO2 extraction.
Determination of flavonoids content in coloured peas (Pisum sativum L.) In relation to cultivar’s dependence and storage duration under natural conditions
Authors: Timoracká Mária , Vollmannová Alena 
Published: July 2010

Our attention is being given especially to four representative flavonoids: kaempherol, apigenin, genistein and daidzein. These flavonoids were studied in the seeds of colored varieties of dry pea. Isocratic HPLC analysis with DAD detection after acid hydrolysis of samples was performed. The differences of flavonoid contents in individual pea varieties were not significant. The determined values of flavonoids in green peas were: daidzein 1,746-2,688 mg.kg-1, genistein 0,412-0,706 mg.kg-1, kaempherol 0,621-1,484 mg.kg-1, apigenin 0,261-0,479 mg.kg-1. Yellow varieties of pea contained between 0,375-0,779 mg.kg-1 daidzein, 0,115-0,158 mg.kg-1 genistein, kaempherol 0,742-1,314 mg.kg-1, apigenin 0,462-0,698 mg.kg-1. Also the changes in content of chosen flavonoids in pea in the dependence on variety in dried legumes stored under natural conditions were surveyed. From the results it came out that in interval 7 month the content of all observed flavonoids declined in dry material (in some cases even to non-detectable levels).
Aligning Single‐Walled Carbon Nanotubes By Means Of Langmuir–Blodgett Film Deposition: Optical, Morphological, and Photo‐electrochemical Studies
Authors: Giancane Gabriele , Ruland Andrés , Sgobba Vito , Manno Daniela , Serra Antonio , Farinola Gianluca M. , Omar Omar Hassan , Guldi Dirk M. , Valli Ludovico 
Published: July 2010

AbstractAn alkoxy‐substituted poly(phenylene thiophene) is used in order to suspend single‐walled carbon nanotubes in an organic solvent. The suspension is spread on the air–water interface of a Langmuir trough and the floating film is characterized by means of Brewster angle microscopy and UV‐visible reflection spectroscopy and the compression isotherm is recorded. The polymer/carbon‐nanotube blend is transferred onto different substrates using the Langmuir–Blodgett technique. AFM measurements indicate the formation of globular structures for the samples transferred at low surface‐pressure values and a tubular morphology for high‐pressure‐deposited samples. AFM analysis is repeated on a sample exposed to soft X‐rays for about 5 h and a highly organized structure of bundles of carbon nanotubes rises up. Samples with different numbers of layers are transferred onto ITO substrates by means of the Langmuir–Blodgett method and are tested as photocathodes in a photo‐electrochemical cell. A Voc of 0.18 V, an Isc of 85.8 mA, FF of 40.0%, and η of (6.23 × 10−3)% are obtained.
Entanglement in fermionic chains with interface defects
Authors: Eisler V. , Peschel I. 
Published: July 2010

AbstractWe study the ground‐state entanglement of two halves of a critical transverse Ising chain, separated by an interface defect. From the relation to a two‐dimensional Ising model with a defect line we obtain an exact expression for the continuously varying effective central charge which governs the asymptotic behaviour of the entanglement entropy. The result is relevant also for other fermionic chains.