bluuebunny/crossref_metadata_2025_split huggingface dataset:
Onychomatricoma
Authors: Durrant Michael N. , Palla Beth A. , Binder Scott W. 
Published: October 2011

Onychomatricoma (OM) is a fibroepithelial tumor of nail matrix that occurs in the digits of both the hands and feet. This was first reported by Baran and Kint. They initially described 3 cases, all of which demonstrated a filamentous tumor of matrix tissue that resulted in a thickened funnel-shaped nail. Although apparently benign, it is subject to recurrence, and long-term follow-up is recommended because it is not known whether there is a conversion to malignancy. Even though this neoplasm was first described more than 18 years ago, there remains a dearth of case reports (currently fewer than 50) in the literature. This is a single case report and literature review. Levels of Evidence: Therapeutic, Level IV
Mechanisms of antigen presentation to T cells in murine graft-versus-host disease: cross-presentation and the appearance of cross-presentation
Authors: Wang Xiaojian , Li Hongmei , Matte-Martone Catherine , Cui Weiguo , Li Ning , Tan Hung Sheng , Roopenian Derry , Shlomchik Warren D. 
Published: October 2011

AbstractRecipient antigen-presenting cells (APCs) initiate GVHD by directly presenting host minor histocompatibility antigens (miHAs) to donor CD8 cells. However, later after transplantation, host APCs are replaced by donor APCs, and if pathogenic CD8 cells continue to require APC stimulation, then donor APCs must cross-present host miHAs. Consistent with this, CD8-mediated GVHD is reduced when donor APCs are MHC class I−. To study cross-presentation, we used hosts that express defined MHC class I Kb-restricted miHAs, crossed to Kb-deficient backgrounds, such that these antigens cannot be directly presented. Cross-priming was surprisingly efficient, whether antigen was restricted to the hematopoietic or nonhematopoietic compartments. Cross-primed CD8 cells were cytolytic and produced IFN-γ. CD8 cells were exclusively primed by donor CD11c+ cells, and optimal cross-priming required that they are stimulated by both type I IFNs and CD40L. In studying which donor APCs acquire host miHAs, we made the surprising discovery that there was a large-scale transfer of transmembrane proteins from irradiated hosts, including MHC class I–peptide complexes, to donor cells, including dendritic cells. Donor dendritic cells that acquired host MHC class I–peptide complexes were potent stimulators of peptide-specific T cells. These studies identify new therapeutic targets for GVHD treatment and a novel mechanism whereby donor APCs prime host-reactive T cells.
Comparative transcriptome sequencing of germline and somatic tissues of the Ascaris suum gonad
Authors: Ma Xuan , Zhu Yingjie , Li Chunfang , Shang Yunlong , Meng Fanjing , Chen Shilin , Miao Long 
Published: October 2011

Abstract

Background

Ascaris suum (large roundworm of pigs) is a parasitic nematode that causes substantial losses to the meat industry. This nematode is suitable for biochemical studies because, unlike C. elegans, homogeneous tissue samples can be obtained by dissection. It has large sperm, produced in great numbers that permit biochemical studies of sperm motility. Widespread study of A. suum would be facilitated by more comprehensive genome resources and, to this end, we have produced a gonad transcriptome of A. suum.

Results
Two 454 pyrosequencing runs generated 572,982 and 588,651 reads for germline (TES) and somatic (VAS) tissues of the A. suum gonad, respectively. 86% of the high-quality (HQ) reads were assembled into 9,955 contigs and 69,791 HQ reads remained as singletons. 2.4 million bp of unique sequences were obtained with a coverage that reached 16.1-fold. 4,877 contigs and 14,339 singletons were annotated according to the C. elegans protein and the Kyoto Encyclopedia of Genes and Genomes (KEGG) protein databases. Comparison of TES and VAS transcriptomes demonstrated that genes participating in DNA replication, RNA transcription and ubiquitin-proteasome pathways are expressed at significantly higher levels in TES tissues than in VAS tissues. Comparison of the A. suum TES transcriptome with the C. elegans microarray dataset identified 165 A. suum germline-enriched genes (83% are spermatogenesis-enriched). Many of these genes encode serine/threonine kinases and phosphatases (KPs) as well as tyrosine KPs. Immunoblot analysis further suggested a critical role of phosphorylation in both testis development and spermatogenesis. A total of 2,681 A. suum genes were identified to have associated RNAi phenotypes in C. elegans, the majority of which display embryonic lethality, slow growth, larval arrest or sterility.

Conclusions
Using deep sequencing technology, this study has produced a gonad transcriptome of A. suum. By comparison with C. elegans datasets, we identified sets of genes associated with spermatogenesis and gonad development in A. suum. The newly identified genes encoding KPs may help determine signaling pathways that operate during spermatogenesis. A large portion of A. suum gonadal genes have related RNAi phenotypes in C. elegans and, thus, might be RNAi targets for parasite control.
Mutations in WDR62 gene in Pakistani families with autosomal recessive primary microcephaly
Authors: Kousar Rizwana , Hassan Muhammad Jawad , Khan Bushra , Basit Sulman , Mahmood Saqib , Mir Asif , Ahmad Wasim , Ansar Muhammad 
Published: October 2011

Abstract

Background
Autosomal recessive primary microcephaly is a disorder of neurogenic mitosis that causes reduction in brain size. It is a rare heterogeneous condition with seven causative genes reported to date. Mutations in WD repeat protein 62 are associated with autosomal recessive primary microcephaly with cortical malformations. This study was initiated to screen WDR62 mutations in four consanguineous Pakistani families with autosomal recessive primary microcephaly.

Methods
As part of a large study to detect the genetic basis of primary microcephaly in Pakistan, homozygosity mapping and DNA sequencing was used to explore the genetic basis of autosomal recessive primary microcephaly in four families.

Results
Four out of 100 families recruited in the study revealed linkage to the MCPH2 locus on chromosome 19, which harbor WDR62 gene. DNA sequencing in these MCPH2 linked families result in the identification of a novel nonsense mutation (p.Q648X) and three previously known mutations.

Conclusion
Our data indicate that WDR62 mutations cause about 4% of autosomal recessive primary microcephaly in Pakistan.
Testicular function and physical outcome in young adult males diagnosed with idiopathic 46 XY disorders of sex development during childhood
Authors: Blanc Thomas , Ayedi Ahmed , El-Ghoneimi Alaa , Abdoul Hendy , Aigrain Yves , Paris Françoise , Sultan Charles , Carel Jean-Claude , Léger Juliane 
Published: October 2011

ObjectiveThere are few studies of outcome in male patients with undefined 46 XY disorder of sex development (DSD). We aimed to assess testicular function and clinical characteristics after puberty in men with idiopathic 46 XY DSD.DesignWe conducted a University Hospital-based observational follow-up study.MethodsNineteen patients with severe hypospadias associated with other signs of defective virilization, such as microphallus, cryptorchidism, and/or bifid scrotum, who were initially managed during childhood between 1988 and 1994, were evaluated at a median age of 17.6 (16.3; 17.8) years. Outcome measures included clinical findings and serum testosterone, FSH, LH, and inhibin B concentrations.ResultsTesticular function was normal in only five (26%) patients. Impaired testicular function was observed in 14 (74%) patients and was partial (n=6; 32%) or total (n=8; 42%), requiring testosterone treatment for the initial (n=2) or secondary (n=6) induction of puberty. Undescended testis (unilateral n=3, bilateral n=2) was found and surgically managed only in the 14 patients with testicular impairment. Testosterone treatment in early childhood greatly increased penis length in all patients, but persistent microphallus following surgical treatment was observed at the end of puberty in most patients, with no difference between patients with and without testicular dysfunction (penis length of 68 (60; 75) vs 65 (60; 65) mm; P=0.42). Half the patients presented an adult height more than 5 cm below their target height.ConclusionMen diagnosed with idiopathic 46 XY DSD during childhood are at high risk of testicular insufficiency and persistent micropenis, and this should be taken into account during the follow-up.
Preliminary Report: Vitamin D Deficiency in Advanced Cancer Patients with Symptoms of Fatigue or Anorexia
Authors: Dev Rony , Del Fabbro Egidio , Schwartz Gary G. , Hui David , Palla Shana L. , Gutierrez Noah , Bruera Eduardo 
Published: October 2011

AbstractBackground.Vitamin D deficiency in noncancer patients is associated with symptoms of fatigue, muscle weakness, and depression. These symptoms are common among advanced cancer patients. We investigated the prevalence of low serum vitamin D levels in cancer patients with fatigue or poor appetite and their association with symptom burden and other correctable endocrine abnormalities.Methods.This was a retrospective review of 100 consecutive cancer patients with appetite or fatigue scores of ≥4 of 10 referred to a supportive care clinic. We investigated serum levels of 25(OH) vitamin D, cortisol, thyroid-stimulating hormone, and bioavailable testosterone. Symptoms were measured by the Edmonton Symptom Assessment Scale. Serum 25(OH) vitamin D &lt;20 ng/mL was considered deficient; ≥20 ng/mL and &lt;30 ng/mL were considered insufficient.Results.Patients were predominantly male (68%) and white (66%), with a median age of 60 years (range, 27–91 years). Gastrointestinal (30%) and lung (22%) cancers were predominant. Forty-seven patients (47%) were vitamin D deficient and 70 (70%) were insufficient. Thirteen of 70 patients (19%) with vitamin D insufficiency were on supplementation. Vitamin D deficiency was more common among nonwhites (82% versus 36%) and females. No significant association was found between vitamin D and symptoms. Hypogonadic males had a significantly lower mean 25(OH) vitamin D level than eugonadic males.Conclusions.Low vitamin D levels were highly prevalent among advanced cancer patients with cachexia or fatigue. Vitamin D deficiency was more frequent among nonwhite and female patients. Vitamin D levels were also significantly lower in male patients with hypogonadism.
Cartilage thickness of the trochlea of the talus, with emphasis on sites predisposed to osteochondrosis dissecans, in clinically normal juvenile and adult dogs
Authors: Brunnberg Mathias M. , Engelke Elisabeth , Gielen Ingrid M. , van Bree Henri J. , Hoffmann Jan E. , Brunnberg Leo , Waibl Helmut R. 
Published: October 2011

Abstract
Objective—To evaluate cartilage thickness of the talus (especially at sites predisposed to osteochondrosis dissecans [OCD]) in growing and adult dogs not affected with OCD.
Sample—Tarsocrural joints from cadavers of 34 juvenile (approx 3 months old) and 10 adult dogs.
Procedures—Tarsal cartilage thickness was examined via a stereophotography microscopic system. Articular cartilage thickness was determined at 11 locations on longitudinal slices of the trochlear ridges and the sulcus between the ridges and at 2 locations in the cochlea tibiae. Cartilage thickness was measured at the proximal, proximodorsal, dorsal, and distal aspects of the trochlear ridges; proximodorsal, dorsal, and distal aspects of the trochlear sulcus; and craniolateral and caudomedial aspects of the cochlea tibiae. Differences within a joint and between sexes were evaluated.
Results—Mean cartilage thickness decreased from proximal to distal in juvenile (lateral trochlear ridge, 1.52 to 0.41 mm; medial trochlear ridge, 1.10 to 0.40 mm) and from proximal to dorsal in adult (lateral trochlear ridge, 0.41 to 0.34 mm; medial trochlear ridge, 0.33 to 0.23 mm) dogs. Cartilage was thickest at the proximal aspect of the lateral trochlear ridge in both groups. Differences in proximodorsal, dorsal, and distal aspects of the ridges were not evident.
Conclusions and Clinical Relevance—Healthy tarsocrural joints did not have thicker cartilage in sites predisposed to development of OCD. Evaluation of affected tarsocrural joints is necessary to exclude influences of cartilage thickness. These data are useful as a reference for distribution of cartilage thickness of the trochlea of the talus in dogs.
Cystic ectopic lingual thyroid tissue in a male cat
Authors: Reed Travis P. , Brisson Brigitte A. , Schutt Leah K. 
Published: October 2011

Abstract
Case Description—A 6-year-old neutered male cat was examined because of a 4-week history of abnormal sounds while drinking and a previously noted mass at the base of the tongue.
Clinical Findings—Oral examination revealed a 1-cm-diameter midline cystic mass on the dorsal aspect of the base of the tongue at the junction of the rostral two-thirds and caudal third of the tongue. Complete blood count and serum biochemical analysis revealed no clinically relevant abnormalities, and serum total thyroxine and free thyroxine (determined by equilibrium dialysis) concentrations were within the reference range.
Treatment and Outcome—The fluid in the cystic mass was aspirated, and the remaining deflated mass was marsupialized. Histologic and immunohistochemical examination of sections of the excised mass revealed ectopic thyroid tissue. The cat recovered uneventfully from the surgery, clinical signs resolved, and the cat remained euthyroid with no recurrence of the mass as of 8 months after surgery.
Clinical Relevance—This is the first known reported case of ectopic lingual thyroid tissue in a male cat. In humans, the most common site of ectopic thyroid tissue is at the base of the tongue and the condition is disproportionately found in females, compared with males. In humans with ectopic lingual thyroid tissue, the patient often lacks any other functional thyroid tissue. However, the cat of this report remained euthyroid after mass resection.
Intralesional injection of insulin-like growth factor-I for treatment of superficial digital flexor tendonitis in Thoroughbred racehorses: 40 cases (2000–2004)
Authors: Witte Thomas H. , Yeager Amy E. , Nixon Alan J. 
Published: October 2011

Abstract
Objective—To evaluate outcome after intralesional injection of insulin-like growth factor-I (IGF-I) for treatment of superficial digital flexor (SDF) tendonitis in Thoroughbred racehorses.
Design—Retrospective case series.
Animals—40 Thoroughbred racehorses.
Procedures—Medical records of racehorses with SDF tendonitis treated within 13 weeks after injury by intralesional injection of IGF-I (25 or 50 μg every other day for 4 or 5 treatments) were reviewed. Outcome was determined via analysis of race records, owner follow-up, and examination.
Results—Mean age of the horses was 3.1 years (range, 2 to 7 years), and time from injury to treatment ranged from 8 to 90 days. Mean ± SD approximate lesion length on admission was 15.6 ± 6.0 cm, and mean percentage cross-sectional area of the tendon affected was 26 ± 18%. Twenty-six of the 40 horses underwent desmotomy of the accessory ligament of the SDF tendon. Echolucency was reduced in 23 of 26 horses by the end of the treatment period. Twenty-one of 34 (62%) horses for which race data were available raced at least once after treatment, including 10 (30%) horses that raced between 1 and 4 times and 11 horses (32%) that raced ≥ 5 times. Thirteen of 28 (46%) horses had a recurrence of tendonitis or developed tendonitis elsewhere.
Conclusions and Clinical Relevance—Results suggested that in Thoroughbred racehorses with SDF tendonitis, intralesional injection of IGF-I led to a decrease in ultrasonographic lesion severity, but treated horses had only a moderate prognosis for return to racing.
Complex patterns of autopolyploid evolution in alfalfa and allies (Medicago sativa; Leguminosae)
Authors: Havananda Tee , Brummer E. Charles , Doyle Jeff J. 
Published: October 2011

• Premise of the study: Although there is growing evidence that autopolyploidy is a widespread and important evolutionary phenomenon, it has received less attention than allopolyploidy. Medicago sativa comprises several diploid and autopolyploid taxa, including autotetraploid cultivated alfalfa, and affords an opportunity to elucidate the evolutionary history of a morphologically and genetically complex autopolyploid system.• Methods: Phylogenies and haplotype networks were constructed from two chloroplast noncoding regions (rpl20‐rps12 and trnS‐trnG spacers) across seven diploid and polyploid infraspecific taxa of M. sativa and five additional closely related Medicago species, and genetic differentiation was estimated.• Key results: The two most prominent M. sativa autopolyploids have contrasting evolutionary histories. Chloroplast data support a simple autopolyploid origin of subsp. sativa (alfalfa) from diploid subsp. caerulea, from which it is distinguishable in several quantitative characters. In contrast, morphologically identical diploid and autopolyploid cytotypes of subsp. falcata were found to possess very different chloroplast haplotypes, suggesting past introgression from M. prostrata into the polyploid. Despite the presence of hybrids between tetraploid subspecies falcata and sativa, there was little evidence of introgression of chloroplast genomes from either subspecies into the other.• Conclusions: Autopolyploid evolution in M. sativa is complicated and has followed very different paths in different subspecific taxa. The potential exists for gene flow in virtually all combinations of subspecies both within and between ploidies, yet despite the existence of hybrids, morphologically and genetically distinctive subspecies persist.
Biogeographic disjunction between eastern Asia and North America in the Adiantum pedatum complex (Pteridaceae)
Authors: Lu Jin‐Mei , Li De‐Zhu , Lutz Sue , Soejima Akiko , Yi Tingshuang , Wen Jun 
Published: October 2011

• Premise of the study: Biogeographic analyses of ferns with an eastern Asian–North American disjunction are few. The Adiantum pedatum complex has such a disjunct distribution. The monophyly of the complex needs to be tested and diversification history of the four species needs to be reconstructed.• Methods: Plastid (atpA, atpB, rbcL, trnL‐F, and rps4‐trnS) sequences of 100 accessions representing the biogeographic diversity of Adiantum were analyzed with parsimony and Bayesian inference. Biogeography of the Adiantum pedatum complex was inferred using programs DIVA and LAGRANGE. Divergence times of clades were estimated with the program BEAST.• Key results: The A. pedatum complex is monophyletic and sister to the eastern Asian A. edentulum. Accessions of A. pedatum do not form a clade; instead three subgroups are recognizable. The clade of A. aleuticum and A. viridimontanum is nested within A. pedatum. The Asian A. myriosorum is sister to the A. pedatum‐A. aleuticum clade. Both DIVA and LAGRANGE analyses suggest an eastern Asian origin of the A. pedatum complex. The age of the crown A. pedatum complex is dated to be at 4.27 (2.24–6.57) million years ago.• Conclusions: The currently recognized eastern Asian–North American disjunct species A. pedatum needs to be segregated into three species, corresponding to populations in eastern North America, China, and Japan. The eastern Asian–North American disjunction in the complex is inferred to be the result of two intercontinental migrations, one from eastern Asia into North America in the late Tertiary and the other from North America back to eastern Asia in the Pleistocene.
Should Anti-citrullinated Protein Antibody and Rheumatoid Factor Status Be Reassessed During the First Year of Followup in Recent-Onset Arthritis? A Longitudinal Study
Authors: MJAAVATTEN MARIA D. , van der HEIJDE DÉSIRÉE M. , UHLIG TILL , HAUGEN ANNE J. , NYGAARD HALVOR , BJØRNEBOE OLAV , KVIEN TORE K. 
Published: October 2011

Objective.Presence and levels of antibodies contribute to the classification of rheumatoid arthritis. We investigated the longitudinal course of anti-citrullinated protein antibodies (ACPA) and immunoglobin M (IgM) rheumatoid factor (RF) during the first year after arthritis onset in patients with very short disease duration.Methods.Patients (aged 18–75 years) with ≥ 1 swollen joint of ≤ 16 weeks’ duration had assessments of ACPA (2nd generation anti-cyclic citrullinated peptide antibodies, anti-CCP2) and IgM RF at inclusion and after 3, 6, and 12 months. Frequencies of seroconversions (negative to positive and vice versa) and changes in antibody levels during followup were determined.Results.A total of 281 early arthritis patients (median duration of joint swelling 32 days, 14.2% ACPA positives, 12.8% IgM RF positives) with 978 longitudinally collected serum samples were included. Only 5 patients (1.8%) negative for both antibodies at baseline turned antibody-positive during followup, while 9 antibody-positive patients (3.2%) turned antibody-negative. ACPA was more stable than RF regarding both status and levels.Conclusion.Antibody status (ACPA/RF) is a stable phenotype in very early arthritis, as seroconversion was only found in 5% of patients. Repeated measurement of ACPA or RF during the first year after onset of arthritis does not offer major additional information.
L'homme aux prises avec le sexuel génital
Authors: Schwering Karl-Leo 
Published: October 2011

Les catégories du féminin et du masculin se construisent au fil de la vie, et sont loin d’être constituées au sortir de l’adolescence. Le texte s’intéresse tout particulièrement aux manifestations sexuelles du masculin génital. Il s’appuie largement sur les thèses de J. Schaeffer consacrant le principe d’une sexualité de jouissance opposée à une sexualité de satisfaction . La première emprunterait au modèle de l’organisation anale et déboucherait sur une pratique sexuelle s’appuyant avant tout sur les pulsions partielles et les objets correspondants (idéalisation du seul pénis érigé, par exemple). La sexualité de jouissance dépasserait ce modèle en se prêtant pleinement à une pulsion constante débouchant sur une pratique sexuelle réussissant à la fois l’intégration des objets partiels (le « pénis libidinal » exaltant tous les autres objets investis au cœur de l’acte sexuel), autant que l’élaboration des fantasmes liés aux imagos parentales et à la scène primitive. Un cas clinique est proposé afin d’illustrer ce « travail du masculin » toujours en construction.
A New View: Tele–Intensive Care Unit Competencies
Authors: Goran Susan F. 
Published: October 2011

BackgroundMany hospitals have well-planned nursing competency assessment programs, but these are meant to measure competency in traditional bedside roles, not in tele–intensive care unit (tele-ICU) nurses practicing remotely.ObjectiveTo determine whether current tele-ICU programs have a formal competency assessment program and to determine when and how competency of tele-ICU nurses is assessed.MethodA 20-question survey was provided to a convenience sample of the 44 known tele-ICU programs nationally.ResultsOf the surveys distributed, 75% were completed and returned. A formal competency assessment policy for assessing nurses’ competency at the time of hire, during orientation, and ongoing was in place at the workplaces of 85% of respondents. The most common methods for competency validation were performance appraisal and observation, although peer review and self-assessment also were used. Respondents identified the following competencies as the highest priorities for defining tele-ICU nurse practice: effective listening, prioritization, collaboration, and effective use of tele-ICU application tools.ConclusionAlthough awaiting development of professional practice standards, many tele-ICU programs currently measure the competence of tele-ICU nurses through competency programs.