bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sequencing analysis of anti mullerian hormone in polycystic ovarian syndrome and primary ovarian insufficiency
Authors: Ermanto Budi , Fadilah  , Bowolaksono Anom , Asmarinah  , Djuwantono Tono , Kekalih Aria , Maidarti Mila , Kusuma Wisnu Ananta , Wiweko Budi 
Published: May 2024

Link of Video Abstract: https://youtu.be/HvI9j8B9vqE

Introduction: Polycystic ovarian syndrome (PCOS) is the most common endocrine disorder in women of reproductive age and is the main cause of ovulation disorders. Primary ovarian insufficiency (POI) is a clinical syndrome characterized by loss of ovarian activity before the age of 40 years. All of these disorders lead to the risk of infertility. Therefore, this study aims to assess the AMH gene sequence and the effect of SNP on AMH levels and function.
Method: This research was a cross-sectional study. The sample was divided into three groups such as PCOS, POI, and control. This study used ELISA and PCR examination methods. Research data were analyzed using IBM SPSS version 22. The statistical analyses used were the ANOVA, Kruskal-Wallis, chi-square, and Spearman tests. A significant p-value was ≤ 0.05.
Results: There were 31 research subjects, 8 POI subjects, 16 PCOS subjects, and 7 control subjects. Overall 30 mutations were found, with two mutations in the gene promoter and 28 mutations in the structure of the AMH gene. Five mutations in the AMH gene structure were missense mutations. 14 SNPs were found in the POI group and 15 SNPs in the PCOS group. There was a significant difference in AMH AMH gene promoter mutation frequency distribution between the POI and PCOS groups at the mutation point 19:g.2249146T>G (p=0.007). In contrast, the mutation point 19:g.2249158T>A was not significantly different (p=0.536). There was no significant correlation between the number of promoter mutations and AMH gene structure with AMH levels in the POI, PCOS and control groups.
Conclusion: There were mutations in the promoter and AMH gene structure of the POI, PCOS, and control groups which caused differences in base sequences. However, in this study, the differences in mutations were not significantly different.
Giant cell tumor of the thoracal spine treated with decompression and posterior stabilization: a case report
Authors: Biakto Karya Triko , Jainal Arifin  , Budiharto Rheza Setiawan , Kennedy Dave 
Published: May 2024

Link of Video Abstract: https://youtu.be/9d-gkVfVFX8

Background: Giant cell tumors (GCT) are benign tumors that can also develop in the bone. GCTs commonly affect women aged 30-40 years. The spine is a rare site of GCTs, which accounts for 1.4-9.4% of primary spine tumors. Therefore, we aimed to present a case of spinal GCT in a young male adult, which we considered unique due to the unusual age and localization.
Case presentation: We reported an 18-year-old male admitted to Hasanuddin University General Hospital with complaints of paralysis in both lower limbs, lower back pain, loss of sensation in defecation, difficulty in urinating, and tenderness at the T12 level. There was a wound on the sacral region. The mass and osteolytic lesion in the T12 seen on CT scan and MRI, respectively, followed by a biopsy, confirmed the spinal GCT.
Conclusion: The management of spinal GCT requires precise and multidisciplinary treatment planning with a case-by-case approach in order to achieve total or partial tumor resection, neural decompression, and stabilization of the spine.
Phenotypic and genotypic of gram-positive coccus β-hemolysis bacteria isolates from tonsil and nasal of pig and antimicrobial susceptibility test against penicillin G and tetracycline
Authors: Pinatih Komang Januartha Putra , Astuti Komang Tri , Suardana I Wayan , Sukada I Made , Salasia Siti Isrina Oktavia , Supadmanaba I Gede Putu , Wihandani Desak Made 
Published: May 2024

Background: The nasal cavity of a pig serves as an entry point and a habitat for the colonization of commensal microbes and pathogenic bacteria such as bacterial zoonosis. This study aimed to identify the phenotypic and genotypic of Gram-positive β-hemolytic organisms collected from nasal and tonsil swabs of pigs that were collected from the abattoir surrounding outbreak area, complete with testing sensitivity test of the bacterial isolates to Penicillin G and Tetracycline antibiotics.
Material and Methods: Totaling 18 isolates consisting of 9 isolates from nasal and 9 isolates from tonsil of coccus β-Hemolysis bacteria originating from 60 samples were used. These isolates were then conventionally identified, then molecularly using 16S rRNA gene analysis. The sensitivity test was carried out by the Kirby Bauer method following the standards of the Clinical and Laboratory Standards Institute (CLSI).
Results: The results of the phenotypic identification (biochemical test) revealed that 9 out of 18 isolates were identified as Enterococcus spp. Furthermore, the confirmation of isolates by molecular analysis i.e. the 16S rRNA gene showed consistency with the biochemical test, and there was confirmed as Enterococcus faecium and Enterococcus faecalis. The sensitivity test showed as many as 9 isolates resistant to Penicillin G and Tetracycline, 5 isolates were resistant, 1 isolate was sensitive and 3 were intermediates. Moreover, the results of the sensitivity test to Penicillin G and Tetracycline, 5 out of 9 isolates showed resistance to more than one antibiotic.
Conclusion: The phenotypic and genotypic of Gram-Positive Coccus β-Hemolysis bacteria isolates from the tonsil and nasal of pigs showed as Enterococcus faecium and Enterococcus faecalis. The use of antibiotics such as Penicillin G and Tetracycline is not recommended for the treatment of this agent resulting in most of the agents being resistant.
Diagnostic accuracy test of quantitative pupillary light reflex as an indicator of increased intracranial pressure in traumatic brain injury patients: a cross-sectional study
Authors: Santosa Dhania A. , Kusuma Edward , Rehatta Nancy Margarita 
Published: May 2024

Introduction: The gold standard of intracranial pressure (ICP) measurement is an invasive procedure of inserting ICP monitor and one of the limitations of its surrogate, ultrasonography-based (US) optic nerve sheath diameter (ONSD) measurement is operator-dependent. Quantitative pupillary light reflex (PLR) measurement provides objective data promisingly able to predict ICP elevation. The aim of this study was to evaluate diagnostic accuracy of PLR as an indicator of ICP elevation in traumatic brain injury (TBI) patients compared to US-ONSD measurement.
Methods: This was a cross-sectional study involving moderate and severe TBI patients aged 18-65 years without any eye lesion, nor medication and illness that may influence PLR. Healthy volunteers were recruited as control group. ONSD and quantitative PLR measurements (baseline diameter, maximum constriction, constriction velocity, latency and amplitude) were assessed to both groups. The Student t-test or Mann-Whitney U-test was used to compare the ONSD and PLR between case and control group. Further analyses were done to determine the sensitivity, specificity and cut-off value of PLR compared to ONSD in case group.
Result: ONSD measurements or parameters were significantly different between case and control group (p<0.001), while all PLR parameters were significantly different (p<0.005) except for right eye constriction latency. The baseline diameter of the right eye had the highest accuracy with cut-off value of >3.9 mm (specificity of 100%, sensitivity of 50%). Other PLR components had variable in accuracy.
Conclusion: The baseline diameter of the right eye was the most accurate PLR component as an indicator of ICP elevation, represented by ONSD.
The effect of monosodium glutamate on the risk of oral cancer: a systematic review
Authors: Intan Suwandi Siti Nabillah , Anggraini Jamas Ari , Widyaputra Sunardhi 
Published: May 2024

Link of Video Abstract: https://youtu.be/Nk6RJxEL_FU

Introduction: Oral cancer is a malignancy, and most cases are diagnosed as oral squamous cell carcinoma. Oral cancer has a multifactorial etiology, one of which is the pattern of food intake. Monosodium glutamate has been widely used by the public as a flavor enhancer. Until now, the safety of MSG has been debated because several studies state that MSG can have both negative and positive effects, so its effect on the risk of developing oral cancer is still unclear. The aim of this study was to find out the effect of MSG on the risk of oral cancer.
Methods: An article search was performed through the Pubmed, ScienceDirect, EBSCOhost, Nature, Scopus, ResearchGate, and Semantic Scholar databases using predefined keywords, inclusion and exclusion criteria, and adapted to the PICO framework. Article writing refers to the PRISMA guidelines.
Results: A total of five selected articles were obtained. Three in vivo study articles describe the effect of MSG, which can cause genotoxicity, a decrease in the quality and quantity of DNA, and changes in the histological structure of the oral mucosa of experimental animals. One case-control study article explained that MSG had the potential to triple the risk of oral cancer, while one cohort study article described the effect of MSG in suppressing post-chemotherapy side effects in head and neck cancer patients.
Conclusion: MSG has the potential to increase the risk of oral cancer because it tends to be more of a carcinogen. It because MSG can induced and increased oxidative stress, which triggers genotoxicity in oral mucosa cells.
The correlation between oxidative stress markers and increasing intracranial pressure: a study of Malondialdehyde (MDA), Superoxide Dismutase (MnSOD), Nicotinamide Adenine Dinucleotide Phosphate Hydrogen (NADPH), and catalase
Authors: Sadewo Wismaji , Wanandi Septelia Inawati , Rustam Zuherman , Gunawan Kevin , Nugroho Setyo Widi 
Published: May 2024

Link of Video Abstract: https://youtu.be/nINFPi4uVow

Background: Abnormalities in the neurosurgical field concerning cranial structure are almost always followed by increased intracranial pressure (ICP), which causes cerebral hypoxia and disrupts the whole cellular system. This study analyzes the correlation between ICP changes and oxidative stress markers, in subjects with various anatomical pathology abnormalities who underwent surgery.
Methods: This was a cross sectional study of 24 subjects with ICP conducted in the Department of Neurosurgery, Cipto Mangunkusumo Hospital, Jakarta, from November to December 2009. In this study, ANOVA analysis has been done to measure the mean difference oxidative stress (MnSOD, NADPH, MDA, and catalase) in subjects with various changes in ICP who have undergone surgery and Spearman analysis to measure the correlation between each oxidative stress markers. Oxidative stress markers were measured from normal brain tissue taken intra-operatively, cerebrospinal fluid (CSF) taken while doing ventriculostomy, and peripheral blood taken from a central vein catheter (CVC).
Results: The results showed that MDA concentrations in blood were significantly correlated with MDA in brain tissue (P = 0.029) in all of the ICP groups, CSF MnSOD concentration and CSF NADPH concentrations correlated with brain tissue NAPDH (p= 0.000) in congenital group and CSF catalase concentration correlated with brain tissue catalase (p = 0.00) in congenital group.
Conclusion: Increased ICP caused by various pathological conditions causes changes in oxidative stress markers in brain cells, CSF and blood. Oxidative stress markers in blood and CSF are correlated strongly with the brain. It is suggested that it could be used to predict intracranial high pressure in all cases with intracranial abnormalities.
Remodeling in early myocardial infarction: alteration of extracellular matrix; Collagen-1, Collagen-3, α-SMA, and α-Actinin in Porcine heart model
Authors: Putra Muhammad Arza , Alwi Idrus , Soetisna Tri Wisesa , Gunanti  , Sandora Normalina , Busro Pribadi Wiranda , Supomo  , Fitria Nur Amalina , Kusuma Tyas Rahmah 
Published: May 2024

Link of Video Abstract: https://youtu.be/lThOW40upEc

Background: Myocardial infarction (MI) leads to remodeling due to altered cardiac structure and function. The study of MI histopathology in humans or large animals mimicking the human heart is limited. Using the porcine heart model, our study investigated the composition of myocardial extracellular matrix (ECM) in collagen-1, collagen-3, α-smooth muscle actin (α-SMA), and α-actinin before and after myocardial infarction.
Methods: This study used two groups of domestic pigs: the Infarct group (n=4) and the Sham group (n=4). MI was induced by permanent ligation of the proximal branch of the posterior left ventricular artery. Cardiac enzymes, electrocardiography, and echocardiography data were collected before and after ligation. Cardiac tissue was harvested from the infarcted area after 60 minutes of ligation and stained with hematoxylin-eosin and Movat's Pentachrome. Collagen-1, collagen-3, and α-SMA were identified with immunohistochemical labeling, and the labeled area was measured using ImageJ. Meanwhile, α-actinin was visualized using immunofluorescence.
Results: Expression of collagen-1, collagen-3, and α-SMA in the infarct group were significantly decreased after 60 minutes of infarction compared with those in the sham group (p<0.01). The α-actinin was fragmented and diminished in the infarct group.
Conclusion: Myocardial remodeling was detected 60 minutes after infarction with mild alteration in myocardial histoarchitecture and significant deterioration of ECM composition of collagen-1, collagen-3, α-SMA, and fragmented α-actinin fibers in the porcine heart model.
The effect of additional transcutaneous auricular vagus nerve stimulation on back muscle endurance of chronic low back pain
Authors: Kusumastuti Retno Nirmala , Andriana Raden Ayu Meisy , Prawitri Yudith Dian , Tinduh Damayanti , Sugianto Paulus , Melaniani Soenarnatalina 
Published: May 2024

Background: This study aims to investigate the effects of adding transcutaneous auricular vagus nerve stimulation (tVNS) to exercise therapy on back extensor muscle endurance of patients with mechanical chronic low back pain (CLBP).
Methods: A randomized controlled group study was conducted on 22 patients with mechanical CLBP aged 16-55. Participants were randomized into an exercise group (control) and an exercise with a tVNS group (experimental). The outcome was back extensor muscle endurance measured by the Biering Sorensen Test (BST), evaluated before and after 2 weeks of intervention
Results: There was a significant improvement in pre-post evaluation in the intervention group (before: 40.30± 31.20 and after: 66.49±45.87, p<0.05), with a large effect size (Cohen’s d=0.918). No difference in pre-post evaluation in the control group (before: 58.18±50.75; after: 66.82±38.56; p>0.05) with a small effect size (Cohen’s d=0.414). There was no significant difference between the delta BST of the two groups (p=0.418).
Conclusion: Our results suggest that no difference was found between the two groups. However, tVNS addition to exercise therapy showed beneficial effects potency on back muscle endurance of the experimental group in CLBP patients during a relatively short period in two weeks of intervention. Further research is needed to confirm the potential of tVNS therapy in CLBP.
The effect of demographic and clinical characteristics toward ocular surface squamous neoplasia (OSSN) recurrence after tumor excision surgery in Dr. Soetomo General Hospital, Indonesia: A literature review
Authors: Agung Bhakti Wiratama  , Fatmariyanti Susy , Loebis Rozalina 
Published: May 2024

Link of Video Abstract: https://www.youtube.com/watch?v=M4wrqsa21hw

An essential component of the ocular surface is the conjunctiva. The most common non-pigmented malignancy that can affect the conjunctiva is ocular surface squamous neoplasia (OSSN). Despite the vast investigations on this deadly organism, certain elements related to demographics, clinical traits, and recurrence remain unclear. The objective of this review was to explore OSSN in depth. OSSN is classified into several types based on its clinical morphology, such as nodular, plaque, and diffuse OSSN. Plaque types can be classified into gelatinous, leucoplakia, and papilliform. Histopathological classification of OSSN consists of benign, pre-invasive, and invasive types. The OSSN tumor stadium can be classified using the TNM criteria by the American Joint Committee on Cancer (AJCC). OSSN management can be divided into medical and surgical management based on classification and tumor stadium. Despite the advances in the treatment of OSSN, there are still variances and conflicting results in the recurrence after surgery, which certain demographic and clinical types of OSSN might cause. Further study is needed to confirm the significance of the recurrence of each demographic and clinical characteristic of each OSSN tumor.
Synchronous placental site trophoblastic tumor and choriocarcinoma
Authors: Indraprasta Birama Robby , Tjokroprawiro Brahmana Askandar 
Published: May 2024

Link of Video Abstract: https://youtu.be/bx7KdiTKiOQ

Introduction: Gestational trophoblastic neoplasia (GTN) comprises a series of malignancies characterized by abnormal proliferation of fetal trophoblastic tissue. GTN lesions refer to a class of malignant lesions that have distinct histopathologic features, including invasive hydatidiform moles, choriocarcinomas, placental site trophoblastic tumors (PSTT), and epithelioid trophoblastic tumor (ETT). Most often, they occur coexist separately, but there are also cases of mixed GTN with a combination of histologic PSTT, ETT, or choriocarcinoma. PSTT is a rare form of gestational trophoblastic disease (GTD). The percent incidence of this disease is 0.23% of total GTD and 1-2% of total GTN. The incidence rate of choriocarcinoma is also limited. Data collection on the incidence of mixed GTN has been more challenging due to the rarity of this disease.
Case Description: We report a case of a 26-year-old female patient with choriocarcinoma and PSTT. The patient’s beta-human chorionic gonadotropin (β-hCG) levels remained elevated despite treatment with single-agent chemotherapeutic regimen. She underwent a series of chemotherapy treatments and two surgeries were required. The histological examination of the uterine tumor revealed a choriocarcinoma whilst the hysterectomy thereafter showed a PSTT. After the hysterectomy the β-hCG level continued to rise. Due to chemotherapy resistance, EMACO (etoposide, methotrexate, actinomycin-D, cyclophosphamide, vincristine) was changed to EP-EMA (Etoposide, cisplatin, etoposide, methotrexate, actinomycin-D). After two administrations of EP-EMA, β-hCG levels normalized.
Conclusion: This is a rare case of mixed GTN of choriocarcinoma and PSTT. Mixed GTN should be considered when chemotherapy is not effective in the treatment of GTN. These cases need to be reported to improve the diagnosis and management of patients.
Clinical outcomes of Descemet’s stripping endothelial keratoplasty using a manual or automated procedure at Undaan Eye Hospital Surabaya, Indonesia: with one-year follow up
Authors: Afifah Azzahra , Dharmawidiarini Dini , Moenir Farida , Napitupulu Sahata P. H. 
Published: May 2024

Background: Endothelial keratoplasty (EK) is a selective lamellar corneal transplantation technique that replaces damaged endothelium while preserving the patient's corneal stroma. Donor graft preparation can be done using Descemet stripping endothelial keratoplasty (DSEK) or Descemet stripping automated endothelial keratoplasty (DSAEK). Research on EK in Indonesia is currently underreported, but it is crucial for developing strategies to treat corneal blindness in the future. This study aimed to present visual acuity, graft survival, and complications after DSEK or DSAEK at Undaan Eye Hospital Surabaya.
Methods: This study involved 17 eyes, treated with DSEK or DSAEK surgery between 2017 and 2022. Patients with other ocular diseases and incomplete medical records were excluded. Best corrected visual acuity (BCVA) was documented before the surgery, and at 1 day, 1, 3, 6 months, and 1 year after. Data in this study were analyzed using multiple techniques, including Wilcoxon, Kaplan-Meier survival, and bivariate logistic regression analysis.
Results: The postoperative mean BCVA significantly increased to 1.36 ± 0.57 logMAR (p=0.012) at 1 day, 1.02 ± 0.62 logMAR (p=0.002) at 1 month, 0.94 ± 0.66 logMAR (p=0.002) at 3 months, 0.72 ± 0.66 logMAR at 6 months (p=0.000), and 0.74 ± 0.74 logMAR (p=0.001) at 1-year compared to preoperative BCVA of 1.84 ± 0.39 logMAR. The graft could survive 100% within 1 day to 6 months and 76% within 1 year after surgery. Bivariate logistic regression showed donor origin had a statistically significant effect with p-value = 0.031 and odds ratio = 1.8, 95% confidence interval (CI) = 1.003 - 3.229. Corneal edema was the most common complication in the early and late postoperative periods.
Conclusion: DSEK or DSAEK surgery is safe, effective, and viable. It shows good visual outcomes and becomes the most used EK treatment for corneal endothelial dysfunction.
Kadar base excess (be) sebagai prediktor prognosis pada pasien multipel trauma di rumah sakit umum pusat Prof. Dr. I. G. N. G Ngoerah Bali
Authors: Purwanto Angga Prawira , Suwedagatha I Gede , Sudartana I Ketut 
Published: May 2024

Background: Multiple trauma is a condition where a person experiences severe injuries involving two or more organ structures or certain body regions. Based on the Injury Severity Score (ISS), a condition is classified as multiple trauma if it has a score of more than 16 points. In general, multiple trauma conditions cause damage to human tissue and are one of the main causes of death and disability in the world. Base excess BE is a predictor of mortality and can be a marker that can assess adequate resuscitation in multiple trauma patients. Hospitals that provide good care for trauma patients are associated with a good prognosis and low mortality
Method: This is an analytical observational study with a retrospective cohort design in multi-trauma patients at RSUP Prof. Dr. I.G.N.G Ngoerah.
Results: Multiple trauma patients with BE ≤-6 and BE >-6 were male patients with the number of patients being 29 and 23, respectively. The results of the analysis in the study showed that there was a significant relationship between the base excess (BE) variable and the incidence of patient mortality (p<0.001).
Conclusion: Base Excess (BE) in this study can be used as an indicator in determining the mortality of multiple trauma patients, so this examination should be applied in the emergency department of Prof. Hospital. Dr. I.G.N.G Ngoerah.

Latar belakang: Multipel trauma merupakan kondisi dimana seseorang mengalami cedera parah yang melibatkan dua atau lebih struktur organ maupun regio tubuh tertentu. Berdasarkan jumlah Injury Severity Score (ISS), kondisi digolongkan sebagai multipel trauma apabila memiliki skor lebih dari 16 poin. Secara umum, kondisi multiple trauma menimbulkan terjadinya kerusakan jaringan manusia dan merupakan salah satu penyebab utama kematian dan kecacatan di dunia. Base excess BE merupakan prediktor mortalitas dan dapat menjadi suatu marker yang dapat menilai resusitasi yang adekuat pada pasien multipel trauma. Rumah sakit yang memberikan pelayanan pasien trauma yang baik diasosiasikan dengan prognosis yang baik dan mortalitas yang rendah
Metodole: ini merupakan penelitian observasional analitik dengan rancangan yang digunakan kohort retrospektif pada pasien multitrauma di RSUP Prof. Dr. I.G.N.G Ngoerah.
Hasil: Didaptkan pasien multiple trauma dengan nilai BE ≤-6 dan BE >-6 merupakan pasien laki-laki dengan jumlah pasien berturut-turut adalah 29 orang dan 23 orang. Hasil analisis pada penelitian diperoleh adanya hubungan yang bermakna pada variabel base excess (BE) dengan kejadian mortalitas pasien (p<0,001).
Kesimpulan: Base Excess (BE) pada penelitian ini dapat dijadikan sebagai indikator dalam menentukan mortalitas pasien multiple trauma, maka sebaiknya pemeriksaan ini dapat diterapkan di instalasi gawat darurat RSUP Prof. Dr. I.G.N.G Ngoerah.