bluuebunny/crossref_metadata_2025_split huggingface dataset:
Numerical Study of Horizontal Shear Instability Waves along Narrow Cold Frontal Rainbands
Authors: Kawashima Masayuki 
Published: December 2010

Abstract
The effects of variations in low-level ambient vertical shear and horizontal shear on the alongfront variability of narrow cold frontal rainbands (NCFRs) that propagate into neutral and slightly unstable environments are investigated through a series of idealized cloud-resolving simulations.
In cases initialized with slightly unstable sounding and weak ambient cross-frontal vertical shears, core-gap structures of precipitation along NCFRs occur that are associated with wavelike disturbances that derive their kinetic energy mainly from the mean local vertical shear and buoyancy. However, over a wide range of environmental conditions, core-gap structures of precipitation occur because of the development of a horizontal shear instability (HSI) wave along the NCFRs.
The growth rate and amplitude of the HSI wave decrease significantly as the vertical shear of the ambient cross-front wind is reduced. These decreases are a consequence of the enhancement of the low-level local vertical shear immediately behind the leading edge. The strong local vertical shear acts to damp the vorticity edge wave on the cold air side of the shear zone, thereby suppressing the growth of the HSI wave through the interaction of the two vorticity edge waves. It is also noted that the initial wavelength of the HSI wave increases markedly with increasing horizontal shear. The local vertical shear around the leading edge is shown to damp long HSI waves more strongly than short waves, and the horizontal shear dependency of the wavelength is explained by the decrease in the magnitude of the vertical shear relative to that of the horizontal shear.
Vegetation Dynamics within the North American Monsoon Region
Authors: Forzieri Giovanni , Castelli Fabio , Vivoni Enrique R. 
Published: December 2010

Abstract
The North American monsoon (NAM) leads to a large increase in summer rainfall and a seasonal change in vegetation in the southwestern United States and northwestern Mexico. Understanding the interactions between NAM rainfall and vegetation dynamics is essential for improved climate and hydrologic prediction. In this work, the authors analyze long-term vegetation dynamics over the North American Monsoon Experiment (NAME) tier I domain (20°–35°N, 105°–115°W) using normalized difference vegetation index (NDVI) semimonthly composites at 8-km resolution from 1982 to 2006. The authors derive ecoregions with similar vegetation dynamics using principal component analysis and cluster identification. Based on ecoregion and pixel-scale analyses, this study quantifies the seasonal and interannual vegetation variations, their dependence on geographic position and terrain attributes, and the presence of long-term trends through a set of phenological vegetation metrics. Results reveal that seasonal biomass productivity, as captured by the time-integrated NDVI (TINDVI), is an excellent means to synthesize vegetation dynamics. High TINDVI occurs for ecosystems with a short period of intense greening tuned to the NAM or with a prolonged period of moderate greenness continuing after the NAM. These cases represent different plant strategies (deciduous versus evergreen) that can be adjusted along spatial gradients to cope with seasonal water availability. Long-term trends in TINDVI may also indicate changing conditions favoring ecosystems that intensively use NAM rainfall for rapid productivity, as opposed to delayed and moderate greening. A persistence of these trends could potentially result in the spatial reorganization of ecosystems in the NAM region.
Sensitivity of the Aerosol Indirect Effect to Subgrid Variability in the Cloud Parameterization of the GFDL Atmosphere General Circulation Model AM3
Authors: Golaz Jean-Christophe , Salzmann Marc , Donner Leo J. , Horowitz Larry W. , Ming Yi , Zhao Ming 
Published: December 2010

AbstractThe recently developed GFDL Atmospheric Model version 3 (AM3), an atmospheric general circulation model (GCM), incorporates a prognostic treatment of cloud drop number to simulate the aerosol indirect effect. Since cloud drop activation depends on cloud-scale vertical velocities, which are not reproduced in present-day GCMs, additional assumptions on the subgrid variability are required to implement a local activation parameterization into a GCM.This paper describes the subgrid activation assumptions in AM3 and explores sensitivities by constructing alternate configurations. These alternate model configurations exhibit only small differences in their present-day climatology. However, the total anthropogenic radiative flux perturbation (RFP) between present-day and preindustrial conditions varies by ±50% from the reference, because of a large difference in the magnitude of the aerosol indirect effect. The spread in RFP does not originate directly from the subgrid assumptions but indirectly through the cloud retuning necessary to maintain a realistic radiation balance. In particular, the paper shows a linear correlation between the choice of autoconversion threshold radius and the RFP.Climate sensitivity changes only minimally between the reference and alternate configurations. If implemented in a fully coupled model, these alternate configurations would therefore likely produce substantially different warming from preindustrial to present day.
Observations of a Large-Amplitude Internal Wave Train and Its Reflection off a Steep Slope
Authors: Bourgault Daniel , Janes David C. , Galbraith Peter S. 
Published: December 2010

Abstract
Remote and in situ field observations documenting the reflection of a normally incident, short, and large-amplitude internal wave train off a steep slope are presented and interpreted with the help of the Dubreil–Jacotin–Long theory. Of the seven remotely observed waves that composed the incoming wave train, five were observed to reflect. It is estimated that the incoming wave train carried Ei = (24 ± 4) × 104 J m−1 to the boundary. The reflection coefficient, defined as the ratio of reflected to incoming wave train energies, is estimated to be R = 0.5 ± 0.2. This is about 0.4 lower than parameterizations in the literature, which are based on reflections of single solitary waves, would suggest. It is also shown that the characteristics of the wave-boundary situation observed in the field are outside the parameter space examined in previous laboratory and numerical experiments on internal solitary wave reflectance. This casts doubts on extrapolating current laboratory-based knowledge to fjord-like systems and calls for more research on internal solitary wave reflectance.
Effect of Decadal Kuroshio Extension Jet and Eddy Variability on the Modification of North Pacific Intermediate Water
Authors: Qiu Bo , Chen Shuiming 
Published: December 2010

Abstract
Salinity modifications in the North Pacific Intermediate Water (NPIW) core layer of 26.7–26.8 σθ in the western North Pacific Ocean are investigated using temperature–salinity data from available profiling float and hydrographic measurements in 2002–09. During 2002–05, when the Kuroshio Extension (KE) jet was intense and zonally elongated, coherent positive salinity anomalies appeared along the inflow KE jet southeast of Japan and in the downstream Mixed Water region east of 152°E. Broad-scale negative salinity anomalies were detected south of the KE jet and in the upstream Mixed Water region west of 152°E. The signs of these observed salinity anomalies were reversed in 2006–09, when the KE jet transitioned to a weakened and zonally contracted dynamic state. By adopting an isopycnal advection–diffusion model and conducting model runs with the time-dependent advective field inferred from the eddy-resolving satellite altimeter sea surface height data, it is found that the observed salinity anomalies are oscillatory in nature and are determined not only by the decadally varying KE jet itself but also by mesoscale eddy signals that modulate temporally and longitudinally along the path of the KE jet.
Deregulation of Aiolos expression in chronic lymphocytic leukemia is associated with epigenetic modifications
Authors: Billot Katy , Soeur Jérémie , Chereau Fanny , Arrouss Issam , Merle-Béral Hélène , Huang Meng-Er , Mazier Dominique , Baud Véronique , Rebollo Angelita 
Published: December 2010

Abstract
Chronic lymphocytic leukemia (CLL) is characterized by a clonal accumulation of mature neoplastic B cells that are resistant to apoptosis. Aiolos, a member of the Ikaros family of zinc-finger transcription factors, plays an important role in the control of mature B lymphocyte differentiation and maturation. In this study, we showed that Aiolos expression is up-regulated in B-CLL cells. This overexpression does not implicate isoform imbalance or disturb Aiolos subcellular localization. The chromatin status at the Aiolos promoter in CLL is defined by the demethylation of DNA and an enrichment of euchromatin associated histone markers, such as the dimethylation of the lysine 4 on histone H3. These epigenetic modifications should allow its upstream effectors, such as nuclear factor-κB, constitutively activated in CLL, to gain access to promoter, resulting up-regulation of Aiolos. To determine the consequences of Aiolos deregulation in CLL, we analyzed the effects of Aiolos overexpression or down-regulation on apoptosis. Aiolos is involved in cell survival by regulating the expression of some Bcl-2 family members. Our results strongly suggest that Aiolos deregulation by epigenetic modifications may be a hallmark of CLL.
Cocaine-mediated induction of platelet-derived growth factor: implication for increased vascular permeability
Authors: Yao Honghong , Duan Ming , Buch Shilpa 
Published: December 2010

Abstract
Neuroinflammation associated with advanced HIV-1 infection is often exacerbated in cocaine-abusing, HIV-infected patients. The underlying mechanisms could, in part, be attributed to the increased impairment of blood brain barrier integrity in the presence of cocaine. Platelet-derived growth factor (PDGF) has been implicated in several pathologic conditions, specifically attributable to its potent mitogenic effects. Its modulation by drug abuse, however, has received very little attention. In the present study, we demonstrated cocaine-mediated induction of PDGF-BB in human brain microvascular endothelial cells through the binding to its cognate σ receptor. Furthermore, this effect was mediated, with subsequent activation of mitogen-activated protein kinases and Egr-1 pathways, culminating ultimately into increased expression of PDGF-BB. Cocaine exposure resulted in increased permeability of the endothelial barrier, and this effect was abrogated in mice exposed to PDGF-BB neutralizing antibody, thus underscoring its role as a vascular permeant. In vivo relevance of these findings was further corroborated in cocaine-treated mice that were administered neutralizing antibody specific for PDGF-BB as well as in Egr-1−/− mice. Understanding the regulation of PDGF-BB expression may provide insights into the development of potential therapeutic targets for neuroinflammation associated with HIV infection and drug abuse.
Lipid droplets as ubiquitous fat storage organelles in C. elegans
Authors: Zhang Shaobing O , Trimble Rhonda , Guo Fengli , Mak Ho Yi 
Published: December 2010

Abstract

Background
Lipid droplets are a class of eukaryotic cell organelles for storage of neutral fat such as triacylglycerol (TAG) and cholesterol ester (CE). We and others have recently reported that lysosome-related organelles (LROs) are not fat storage structures in the nematode C. elegans. We also reported the formation of enlarged lipid droplets in a class of peroxisomal fatty acid β-oxidation mutants. In the present study, we seek to provide further evidence on the organelle nature and biophysical properties of fat storage structures in wild-type and mutant C. elegans.

Results
In this study, we provide biochemical, histological and ultrastructural evidence of lipid droplets in wild-type and mutant C. elegans that lack lysosome related organelles (LROs). The formation of lipid droplets and the targeting of BODIPY fatty acid analogs to lipid droplets in live animals are not dependent on lysosomal trafficking or peroxisome dysfunction. However, the targeting of Nile Red to lipid droplets in live animals occurs only in mutants with defective peroxisomes. Nile Red labelled-lipid droplets are characterized by a fluorescence emission spectrum distinct from that of Nile Red labelled-LROs. Moreover, we show that the recently developed post-fix Nile Red staining method labels lipid droplets exclusively.

Conclusions
Our results demonstrate lipid droplets as ubiquitous fat storage organelles and provide a unified explanation for previous studies on fat labelling methods in C. elegans. These results have important applications to the studies of fat storage and lipid droplet regulation in the powerful genetic system, C. elegans.
Increased levels of the calcification marker Matrix Gla Protein and the inflammatory markers YKL-40 and CRP in patients with type 2 diabetes and ischemic heart disease
Authors: Thomsen Stine B , Rathcke Camilla N , Zerahn Bo , Vestergaard Henrik 
Published: December 2010

Abstract

Objective and design
Low grade inflammation is of pathogenic importance in atherosclerosis and in the development of cardiovascular disease (CVD) and type 2 diabetes (T2D). Matrix GLA protein (MGP), an inhibitor of medial calcification of arteries, is increased in patients with atherosclerosis. In the present study levels of markers of calcification (MGP) and inflammation (YKL-40, hsCRP) were evaluated in patients with T2 D and/or ischemic heart disease (IHD).

Materials and methods
The study population consisted of 1) patients with T2D (n = 45); 2) patients with IHD (n = 37); patients with both T2D and IHD (n = 20) and 4) healthy controls (n = 20). Biochemical parameters were measured in venous blood samples.

Results
Levels of MGP, YKL-40 and hsCRP were increased in patients with IHD and/or T2D (p < 0.0001) and patients with T2D and IHD had higher MGP levels (p < 0.001). In multiple linear regression analyses MGP was associated with patient category (r = 0.36, p < 0.001), and HDL-cholesterol levels (r = 0.29, p < 0.001) adjusting for the significant covariates.

Conclusions
In patients with T2D and/or IHD we found increased levels of plasma MGP indicative of a progressing calcification process. This process is paralleled by increased levels of YKL-40 and hsCRP, which most likely reflect the concomitant low grade inflammatory state in these patients
Use of cervicovaginal fluid for the identification of biomarkers for pathologies of the female genital tract
Authors: Zegels Geert , Van Raemdonck Geert AA , Tjalma Wiebren AA , Van Ostade Xaveer WM 
Published: December 2010

AbstractCervicovaginal fluid has an important function in the homeostasis and immunity of the lower female genital tract. Analysis of the cervicovaginal fluid proteome may therefore yield important information about the pathogenesis of numerous gynecological pathologies. Additionally, cervicovaginal fluid has great potential as a source of biomarkers for these conditions.This review provides a detailed discussion about the human cervicovaginal proteome and the proteomics studies performed to characterize this biological fluid. Furthermore, infection-correlated pathological conditions of the female genital tract are discussed for which cervicovaginal fluid has been used in order to identify potential biomarkers. Recent years, numerous studies have analyzed cervicovaginal fluid samples utilizing antibody-based technologies, such as ELISA or Western blotting, to identify biomarkers for preterm birth, premature preterm rupture of membranes, bacterial vaginosis and cervical cancer. The present article will discuss the importance of proteomic technologies as alternative techniques to gain additional meaningful information about these conditions. In addition, the review focuses on recent proteomic studies on cervicovaginal fluid samples for the identification of potential biomarkers. We conclude that the use of proteomic technology for analysis of human cervicovaginal fluid samples is promising and may lead to the discovery of new biomarkers which can improve disease prevention and therapy development.
Expression of MDR1, HIF-1α and MRP1 in sacral chordoma and chordoma cell line CM-319
Authors: Ji Zhengang , Long Hua , Hu Yunsheng , Qiu Xiuchun , Chen Xiang , Li Zhao , Fan Degang , Ma Baoan , Fan Qingyu 
Published: December 2010

AbstractBackgroundChordoma was a typically slow-growing tumor. The therapeutic approach to chordoma had traditionally relied mainly on surgical therapy. And the main reason for therapeutic failure was resistance to chemotherapy and radiotherapy. However the refractory mechanism was not clear. The aim of this study was to investigate the expression of three genes (MDR1,HIF-1αandMRP1) associated with resistance to chemotherapy and radiotherapy in chordoma and chordoma cell line CM-319.Materials and methodsUsing immunohistochemical techniques, the expression of MDR1, HIF-1α and MRP1 was investigated in 50 chordoma specimen. Using RT-PCR and Western blot, the expression of MDR1, HIF-1α and MRP1 was investigated in chordoma and chordoma cell line CM-319.ResultsExpression of MDR1, HIF-1α and MRP1 was observed in 10%, 80% and 74% of all cases, respectively. Expression of MRP1 was correlated with HIF-1α. On the other hand, expression of MDR1 was not correlated with the expression of HIF-1α or MRP1. The expression of HIF-1α and MRP1 was observed, but MDR1 was not observed in chordoma and CM-319.ConclusionExpression of HIF-1α and MRP1 was observed in most chordoma specimen and CM-319 cell line; expression of HIF-1α correlated with MRP1. HIF-1α and MRP1 may play a role in the multidrug resistance of chordoma to chemotherapy.
Ambient temperature shapes reproductive output during pregnancy and lactation in the common vole (Microtus arvalis): a test of the heat dissipation limit theory
Authors: Simons Mirre J. P. , Reimert Inonge , van der Vinne Vincent , Hambly Catherine , Vaanholt Lobke M. , Speakman John R. , Gerkema Menno P. 
Published: December 2010

SUMMARYThe heat dissipation limit theory suggests that heat generated during metabolism limits energy intake and, thus, reproductive output. Experiments in laboratory strains of mice and rats, and also domestic livestock generally support this theory. Selection for many generations in the laboratory and in livestock has increased litter size or productivity in these animals. To test the wider validity of the heat dissipation limit theory, we studied common voles (Microtus arvalis), which have small litter sizes by comparison with mice and rats, and regular addition of wild-caught individuals of this species to our laboratory colony ensures a natural genetic background. A crossover design of ambient temperatures (21 and 30°C) during pregnancy and lactation was used. High ambient temperature during lactation decreased milk production, slowing pup growth. The effect on pup growth was amplified when ambient temperature was also high during pregnancy. Shaving fur off dams at 30°C resulted in faster growth of pups; however, no significant increase in food intake and or milk production was detected. With increasing litter size (natural and enlarged), asymptotic food intake during lactation levelled off in the largest litters at both 21 and 30°C. Interestingly, the effects of lactation temperature on pup growth where also observed at smaller litter sizes. This suggests that vole dams trade-off costs associated with hyperthermia during lactation with the yield from investment in pup growth. Moreover, pup survival was higher at 30°C, despite lower growth, probably owing to thermoregulatory benefits. It remains to be seen how the balance is established between the negative effect of high ambient temperature on maternal milk production and pup growth (and/or future reproduction of the dam) and the positive effect of high temperatures on pup survival. This balance ultimately determines the effect of different ambient temperatures on reproductive success.
Whistling in caterpillars (Amorpha juglandis, Bombycoidea): sound-producing mechanism and function
Authors: Bura Veronica L. , Rohwer Vanya G. , Martin Paul R. , Yack Jayne E. 
Published: December 2010

SUMMARYCaterpillar defenses have been researched extensively, and, although most studies focus on visually communicated signals, little is known about the role that sounds play in defense. We report on whistling, a novel form of sound production for caterpillars and rare for insects in general. The North American walnut sphinx (Amorpha juglandis) produces whistle ‘trains’ ranging from 44 to 2060 ms in duration and comprising one to eight whistles. Sounds were categorized into three types: broadband, pure whistles and multi-harmonic plus broadband, with mean dominant frequencies at 15 kHz, 9 kHz and 22 kHz, respectively. The mechanism of sound production was determined by selectively obstructing abdominal spiracles, monitoring air flow at different spiracles using a laser vibrometer and recording body movements associated with sound production using high-speed video. Contractions of the anterior body segments always accompanied sound production, forcing air through a pair of enlarged spiracles on the eighth abdominal segment. We tested the hypothesis that sounds function in defense using simulated attacks with blunt forceps and natural attacks with an avian predator – the yellow warbler (Dendroica petechia). In simulated attacks, 94% of caterpillars responded with whistle trains that were frequently accompanied by directed thrashing but no obvious chemical defense. In predator trials, all birds readily attacked the caterpillar, eliciting whistle trains each time. Birds responded to whistling by hesitating, jumping back or diving away from the sound source. We conclude that caterpillar whistles are defensive and propose that they function specifically as acoustic ‘eye spots’ to startle predators.
Reproductive regulators in decapod crustaceans: an overview
Authors: Nagaraju Ganji Purna Chandra 
Published: December 2010

Summary
Control of reproductive development in crustaceans requires neuropeptides, ecdysone and methyl farnesoate (MF). A major source of neuropeptides is the X-organ–sinus gland (XO–SG) complex located in the eyestalk ganglia of crustaceans. The other regulatory factors (either peptides or neuromodulators) are produced in the brain and thoracic ganglia (TG). Two other regulatory non-peptide compounds, the steroid ecdysone and the sesquiterpene MF, are produced by the Y-organs and the mandibular organs, respectively. In the current review, I have tried to recapitulate recent studies on the role of gonadal regulatory factors in regulating crustacean reproduction.