bluuebunny/crossref_metadata_2025_split huggingface dataset:
Y‐27632 Suppresses the Growth and Migration of Oral Squamous Cell Carcinoma, but Upregulates Autophagy by Suppressing mTOR Effectors
Authors: Wen Jie , Sun Yunhan , Ma Li , Zu Tingjian , Wang Na , Zhang Tianqi , Liang Jin , Zhang Yulei , Lu Haoyang , Wu Yihua , Zhang Shizhou 
Published: February 2025

ABSTRACTBackgroundThe Rho‐associated protein kinase (ROCK) inhibitor Y‐27632 is a potential immunotherapeutic agent for cancer treatment. Y‐27632 blocks the growth and migration of oral squamous cell carcinoma (OSCC) CAL‐27 cells. However, detailed studies on the underlying mechanisms have not yet been reported.MethodsWe investigated the effects of Y‐27632 on the proliferation, migration, and invasion of OSCC cells (CAL‐27, SCC‐4, and SCC‐9) using the Cell Counting Kit‐8 assay, ethynyl‐2′‐deoxyuridine staining, cell scratch, and transwell assay in vitro. Next, ROCK1/2 was knocked down using siRNA to confirm that the effects of Y‐27632 were mediated by the inhibition of ROCK activity. A xenograft mouse model was used to verify the effects of Y‐27632 in vivo. The mechanisms underlying Y‐27632‐induced tumor suppression were detected using western blotting and qRT‐PCR.ResultsOur data demonstrated that Y‐27632 potently inhibited OSCC cells (CAL‐27, SCC‐4, and SCC‐9) by inhibiting ROCK activity. In vivo assays confirmed that Y‐27632 suppressed OSCC growth by reducing cell proliferation. Biochemical assays demonstrated that Y‐27632 inactivated the AKT pathway, and treatment with SC79, an AKT activator, rescued the cell growth and migration inhibition elicited by Y‐27632. Further investigation revealed that Y‐27632 enhanced autophagy by suppressing the AKT/mTOR pathway.ConclusionOur study demonstrated that Y‐27632 significantly suppressed the growth and migration of OSCC cells and upregulated autophagy via the AKT/mTOR pathway, thus providing a potential therapeutic drug for patients with OSCC.
Chewing with Co‐Cr or PEEK denture frameworks: A randomized crossover clinical study
Authors: Oliveira Pedro Henrique Bastos de , Ferreira Guilherme Fantini , Gama Lorena Tavares , Carletti Talita Malini , Rodrigues Garcia Renata Cunha Matheus 
Published: February 2025

AbstractPurposeTo compare masticatory function and patient satisfaction with removable partial dentures (RPDs) with cobalt‐chromium (Co‐Cr) and injected polyetheretherketone (PEEK) frameworks, respectively.Materials and MethodsTwelve volunteers (7 women, 5 men; mean age, 56.6 years) with complete maxillary edentulism and partial mandibular edentulism (Kennedy Class I) first received new RPDs with PEEK (experimental group) or Co‐Cr (control group) frameworks and used them for 2 months. Masticatory performance (MP) was evaluated using sieves and Optocal, with results expressed as the mean particle size (X50); maximum bite force (MBF) was measured with a force transducer and pressure sensors; and self‐reported satisfaction was assessed using a visual analog scale. After the initial evaluation, a 1‐week washout period was followed by group crossover and reassessment after an additional 2 months. Data were analyzed using independent‐samples t‐tests (α = 5%).ResultsX50 values were lower for RPDs with Co‐Cr frameworks than for those with PEEK frameworks (p = 0.007), indicating better MP. The MBF did not differ significantly between RPD framework types. Participants’ satisfaction, in terms of prosthesis stability (p = 0.02) and chewing ability (p < 0.001), was greater for Co‐Cr RPDs. Most participants preferred to continue using Co‐Cr frameworks.ConclusionsRPDs with injected PEEK frameworks were associated with reduced MP and patient satisfaction relative to Co‐Cr frameworks.
Evaluation of different technologies used for extraoral surface data acquisition for 3D facial scanning
Authors: Abdelhakim Nada A. , Salazar‐Gamarra Rodrigo , Segaan Lucette G. , Soliman Ingy S. 
Published: February 2025

AbstractPurposeThis study evaluated the accuracy (trueness and precision) of facial scanners using different technologies and the reliability of the texture maps produced.Materials and MethodsA volunteer was scanned using nine scanners: Cloner, Artec MHT, Revopoint Mini, Revopoint POP 2, Vectra H2, EXAscan, Scaniverse app, iPhone 13 Pro MAX, and Nikon Z 7II Camera. Eighty scans were compared to a reference model (Vectra XT). Root mean square and standard deviation of the dimensional discrepancies were analyzed using ANOVA and Tukey post‐hoc test. Acquisition times were assessed using Kruskal–Wallis test followed by Mann–Whitney U‐test. Two expert assessments on texture map realism were averaged and compared to the reference using one sample t‐test. The two experts' intraclass correlation coefficient (ICC) was calculated.ResultsEight scanners fully captured the three‐dimensional facial geometry. Overall trueness and precision of the facial scanners were significantly different (p < 0.001). Seven scanners showed clinically acceptable scanning accuracy of less than or equal to 2 mm. Cloner displayed the lowest deviation (0.61 ± 0.08 mm), fastest acquisition time (0.40 ± 0.00 s), and was highly reliable (<1 mm). The texture map of the Vectra H2 had the highest mean score of 8.50 and was the most realistic. The ICC between the experts was 0.78 indicating good interexaminer reliability.ConclusionsThe more economical facial scanners within each scanning technology (iPhone 13 Pro MAX, Cloner, Revopoint MINI, and Scaniverse app) showed clinically acceptable accuracy with realistic texture maps for facial scanning and therefore could be eligible substitutes for professional higher‐cost scanners.
Does Industry Agglomeration Attract Productive Firms? The Role of Product Markets in Adverse Selection
Authors: Belderbos René , Fukao Kyoji , Ikeuchi Kenta , Kim Young Gak , Kwon Hyeog Ug 
Published: February 2025

ABSTRACTThe literature has produced mixed findings on the relationship between industry agglomeration and firm‐level productivity where it concerns the self‐selection of productive firms into locations characterized by different levels of industry agglomeration. We argue that the nature of this self‐selection crucially depends on whether incumbent and entrant firms compete on the same market. Adverse selection of less productive firms into a location only dominates if knowledge spillovers in agglomerated locations are harmful to productive entrants: when the entrant and local incumbents target the same (domestic) product market and the entrant risks losing market share and profits. We find evidence for this notion in analysis of location decisions for new plants at the fine‐grained geographical level in Japan by firms with known productivity records in the industry (multi‐plant firms). We conclude that sorting processes do occur, but that the nature of these processes can only be uncovered in analysis that considers competition on product markets and accurate measures of firm heterogeneity in productivity.
Inheritance of Genetic Resistance to Anthracnose in Lima Beans: Analysis and Implications for Breeding
Authors: de Brito Marilha Vieira , Torres Kathully Karolaine Brito , Sousa João Vitor Morais , França Giovana Bezerra , Costa Marcones Ferreira , da Costa Guilherme Alexandre Luz , Ferreira Gerson Nascimento Costa , da Silva Verônica Brito , de Sá Gisele Holanda , Lopes Ângela Celis de Almeida , de Melo Maruzanete Pereira , Gomes Regina Lucia Ferreira 
Published: February 2025

ABSTRACTLima bean (Phaseolus lunatus L.) is a crop of notable agricultural importance. However, its production is severely affected by anthracnose, a disease caused by the fungus Colletotrichum truncatum. This study aimed to investigate the genetic inheritance of anthracnose resistance in lima beans to support breeding efforts. Segregating populations (F1 and F2) derived from crosses between resistant and susceptible genotypes were used. All plants with their first pair of developed leaves were inoculated with a conidia suspension of the CT4 isolate of C. truncatum (106 conidia/mL) to study their inheritance. Phenotypic data were collected and analysed to identify inheritance patterns and resistance loci. According to the chi‐square (χ2) test, the segregating ratio of 1:0 (resistant:susceptible) was accepted for the F1 generation, and the ratio of 3:1 (resistant:susceptible) was accepted in the F2 generation. These results indicate that resistance to C. truncatum in lima beans is conditioned by a single gene, showing evidence of dominant monogenic inheritance. The results offer pathways to develop resistant cultivars, improving crop productivity and sustainability.
Information‐Knowledge‐Attitude‐Practice Model in Non‐Suicidal Self‐Injury Behaviours of Adolescence and Young Adults With Major Depressive Disorder: Randomised Controlled Trials
Authors: Tan Liangliang , Lin Zhengdi , Zhang Yuhua , Sun Nan , Ding Qiu , Feng HaiXia , Jiang Wenhao , Yuan Yonggui 
Published: February 2025

ABSTRACTIntroductionNon‐suicidal self‐injury (NSSI) is considered a cathartic approach to reducing negative emotions; repeated occurrences increase the risk of suicide. Applying an information‐knowledge‐attitude‐practice (IKAP) model might help patients establish effective and sustainable ways to express negative emotions and cope with difficulties, reducing NSSI.AimWe explored an IKAP‐based intervention on NSSI among adolescents and young adult patients with major depressive disorder (MDD).MethodsA total of 120 adolescent and young adult patients with MDD who reported NSSI were recruited from January to December 2021. Using stratified randomisation, the participants were randomly divided into a balanced control group (usual treatment) and intervention group (IKAP intervention). The Cognitive Emotion Scale, NSSI, and handling of dangerous items were assessed pre‐ and post‐intervention.ResultsPost‐intervention, the intervention group had significantly lower NSSI, were more likely to give up dangerous items, and indicated better cognitive‐emotional regulation than the control group (all p < 0.05).DiscussionThe IKAP model significantly improved NSSI‐related outcomes.Implication for PracticeThe IKAP model could improve patients' cognitive‐emotional regulation more than traditional health education, enabling patients to participate more actively in disease management, learn problem‐solving skills, reduce negative emotions, actively cooperate with ward safety management practices, and reduce NSSI.Trial RegistrationChiCTR registration number: ChiCTR2100050317
Three reference genomes for freshwater diatom ecology and evolution
Authors: Roberts Wade R. , Alverson Andrew J. 
Published: February 2025

AbstractDiatoms are an important component of marine and freshwater ecosystems. Although the majority of described diatom species live in freshwater systems, genome sequencing efforts have focused primarily on marine species. Genomic resources for freshwater species have the potential to improve our understanding of diatom ecology and evolution, particularly in the context of major environmental shifts. We used long‐ and short‐read sequencing platforms to assemble reference genomes for three freshwater diatom species, all in the order Thalalassiosirales, which are abundant in the plankton of oceans, lakes, reservoirs, and rivers worldwide. We targeted three species that cover the breadth of phylogenetic diversity in the cyclostephanoid clade of Thalassiosirales: Cyclostephanos tholiformis (JALLPB020000000), Discostella pseudostelligera (JALLBG020000000), and Praestephanos triporus (JALLAZ020000000). The reference genome for D. pseudostelligera was considerably smaller (39 Mb) than those of both P. triporus (73 Mb) and C. tholiformis (177 Mb). Long‐read sequencing allowed for the assembly of scaffold‐level genomes, including regions rich in repetitive DNA. Compared to short‐read assemblies, long‐read assemblies increased the contig N50 length as much as 37‐fold and reduced the number of contigs by more than 88%. Transcriptome‐guided annotation of the protein‐coding genes identified between 10,000 and 12,000 genes. This work provides further demonstration of the value of long‐read sequencing and provides novel genomic resources for understanding the ecology and evolution of freshwater diatoms.
Justice and Contracts as Double‐Edged Swords: Collaborative Product Innovation in Hub‐and‐Spoke Supply Chain Networks
Authors: Liu Gordon , Chen Yantai , Ko Wai Wai 
Published: February 2025

ABSTRACTThis study explores how inter‐organizational justice, and formal contracts influence new product development (NPD) collaboration in supply chain networks. Challenging traditional transaction cost economics (TCE), the research focuses on collaborative NPD in hub‐and‐spoke supply chain structures. Data from 183 Chinese suppliers and 22 executive interviews reveal unexpected patterns in NPD collaboration. Procedural justice exhibits an inverted U‐shaped relationship with NPD collaboration, linking higher fairness to improved collaboration up to a point, beyond which further increases may associate with diminishing returns. In contrast, distributive justice shows a U‐shaped relationship with NPD collaboration, where higher equity initially relates to reduced collaboration but later correlates with renewed engagement. Notably, formal contracts amplify the negative interactions between these justice dimensions. This contradicts the conventional view of their complementary roles. These findings contribute to theoretical advancements by illustrating how inter‐organizational justice mechanisms function differently in complex network structures compared to simple dyadic relationships. Careful calibration of inter‐organizational justice dimensions and formal contracts proves essential for fostering productive NPD collaboration. These governance insights offer directions for enhancing supply chain relationship management.
Co‐production in sleep research: A scoping review of current practices and future directions
Authors: Gale Emma Louise , Manrai Raahat , Caddick Lorna , Murray Aja , Whalley Heather C. , Smith Daniel , Gardani Maria 
Published: February 2025

SummarySleep is essential for mental and physical health, and research in the field has substantially expanded over the past 50 years. Co‐production methodology has been increasingly used within health and social care research, and refers to collaboration between researchers, policy makers, community partners and wider stakeholders. The aim of this scoping review was to detail the use of co‐production methods within sleep research. A review of the existing literature was conducted using seven databases following PRISMA‐ScR guidelines. Search terms included objective and subjective sleep outcomes, and the use of co‐production research methodologies. Sixteen studies were included in the final review: 10 studies used solely qualitative co‐production methods to inform intervention design and development (sleep as a primary outcome [n = 5] and as a secondary outcome [n = 5]), and six studies used co‐production methodologies to establish sleep as a priority outcome for future research. Most studies used consultation approaches to design interventions (n = 8), instead of using co‐design teams (n = 2). Two studies focusing on intervention development recruiting participants from clinical populations with poor sleep, other studies recruited from those with other underlying conditions or a healthy population. The most common limitations of the included studies were small sample size, researcher driven topics/domains for the PAR components, under‐representative samples and COVID‐19 pressures. Future sleep research should consider the use of co‐production methodologies from the study conceptualisation, through to the design, development and implementation of research to further benefit the intended research population.
A cross‐sectional study of the association between sleep disturbance profiles, unmet mental health or substance use needs, and presenteeism among United States activity‐duty service members using the 2018 health‐related behaviours survey (HRBS)
Authors: Russell Teresa L. , Singer Darrell E. , Werner J. Kent , Mancuso James D. , Ahmed Anwar E. 
Published: February 2025

SummaryInadequate sleep, unmet mental health or substance use needs (unmet needs), and presenteeism are prevalent among military populations. This study aimed to cross‐sectionally determine the association between sleep disturbance profiles, unmet needs, and presenteeism in US active‐duty service members, both separately and combined. Data were collected from the 2018 Health‐Related Behaviours Survey. The response rate was 9.6%. Presenteeism was collected as the number of days (0–30) then collapsed for analysis. Latent class analysis (LCA) was used to classify service members into sleep disturbance profiles. Odds ratios and confidence intervals (CIs) were estimated by binary and ordinal logistic models. Approximately 21% of the 17,166 service members reported at least one presentee day (95% CI: 19.8%–21.8%). Persistent presenteeism was 13.6% (95% CI: 12.7–14.4%). Four sleep disturbance profiles were identified by LCA: (1) high sleep disturbance (reported in 22.5%), (2) short sleep duration (26%), (3) trouble sleeping (6.9%), and (4) none to slight sleep disturbance (reference, 44.6%). Female sex, being separated/divorced/widowed, short sleep duration, trouble sleeping, high sleep disturbance, unmet needs, and both unmet needs and inadequate sleep together were associated with higher odds of high presenteeism levels and persistent presenteeism. Bachelor's or higher educated, 25–34‐year‐old, Hispanic/Latinx, Officer, Air Force, and Coast Guard service members were associated with lower odds of high presenteeism levels and persistent presenteeism. Despite the decreasing trends between 2015 and 2018, the high prevalence of presenteeism presents a significant burden on work productivity and readiness that behavioural modification may alter.
Promoting vocabulary learning during sleep at home using closed‐loop targeted memory reactivation
Authors: Salfi Federico , D'Atri Aurora , Arnone Benedetto , Corigliano Domenico , Amicucci Giulia , Viselli Lorenzo , Naccarato Federica , Festucci Fabiana , Tempesta Daniela , Ferrara Michele 
Published: February 2025

SummaryTargeted memory reactivation represents an established technique for promoting sleep‐dependent memory consolidation in laboratory studies. This investigation aimed to test the potentiality of a wearable electroencephalography‐based closed‐loop targeted memory reactivation system to boost vocabulary learning in home settings. In the evening, 24 adults (23.58 years ± 3.36 years, 19 females) were asked to learn the Italian translation of 40 pseudowords (test session). Subsequently, participants slept at their home wearing an electroencephalography headband (Dreem 2), and half of the pseudowords were acoustically re‐presented (cued) following the real‐time detection of slow waves. After the awakening, memory recall of the Italian translations was retested. The stimulation effect was evaluated by comparing the test–retest variations in memory accuracy between cued and uncued pseudowords. Moreover, we assessed the event‐related potentials and spectral perturbations induced by the cued stimuli during sleep, comparing the electrophysiological correlates of correctly translated pseudowords with incorrectly translated ones at the retest session. Closed‐loop targeted memory reactivation increased the translation accuracy for cued pseudowords (+8.6%), while no significant test–retest variation for uncued items was observed (−4.6%). Time–frequency analysis indicated a spectral power increase in the spindle frequency band coinciding with the second positive peak of the sound‐elicited slow wave as the correlate of successful morning recall. This study extended the targeted memory reactivation effectiveness in enhancing vocabulary learning to an ecological home environment, providing further support to the role of spindle activity in the targeted memory reactivation effect. A wearable closed‐loop targeted memory reactivation system could represent a memory‐enhancement tool in real‐world settings by promoting the hallmark sleep electroencephalographic rhythms of memory consolidation.
Plasma and Urine Pharmacokinetics of Long‐Acting Injectable Omeprazole Following Intramuscular Administrations to Healthy Thoroughbred Horses
Authors: Harding Caitlin , Viljanto Marjaana , Hincks Pamela , Habershon‐Butcher Jocelyn , Paine Stuart W. 
Published: February 2025

ABSTRACTOmeprazole is a gastric acid secretion inhibitor used as an effective anti‐ulcer drug. Based on oral administration studies, its International Screening Limit (ISL) was established in plasma and urine at 1 ng/mL with a Detection Time (DT) of 48 h. A novel formulation of injectable omeprazole has since been released, and therefore, a pharmacokinetic study was performed to assess the DT above the ISL against current advice. Six Thoroughbred horses were given four repeated weekly intramuscular administrations of omeprazole (4 mg/kg). Plasma and urine omeprazole concentrations were measured by liquid chromatography–tandem mass spectrometry. Based on the current plasma and urine ISL (1 ng/mL), the DT for this long‐acting omeprazole formulation administered at 4 mg/kg once per week is greater than 384 h (16 days) in both plasma and urine. Thus realistically, despite the appeal of giving an injection once per week rather than oral medication daily over a long period of time, this would make treatment for horses in training with the long‐acting product challenging within the rules of racing. It would therefore most likely be used for horses outside of training, and the oral formulation would still be legitimately used during training.
Disruption and irruption shape genetic variation and population structure of the common rock‐rat in north‐western Australia
Authors: Wale C. H. , McConnell S. , van Leeuwen S. , Cowan M. A. , Spencer P. B. S. , How R. A. , Schmitt L. H. 
Published: February 2025

AbstractEndemic rodents constitute 19% of Australian terrestrial mammal species and this proportion is higher in arid zones and the north. We report substantial genetic diversity and population structure in the common rock‐rat, a saxicoline murid whose range extends across northern Australia and into the continent's interior. Samples of 686 individuals from 68 locations, encompassing the western half of the species' range, provide one of the most geographically and numerically extensive genetic studies of an Australian rodent. The Great Sandy Desert, a prominent feature over the last 0.5 my bp acts as a significant barrier to gene flow. Continental islands, formed during the past 10 000 years following the end of the most recent Pleistocene glacial maximum, have populations with lower heterozygosity and marked differentiation from each other and the adjacent mainland. On the mainland, despite considerable differentiation between locations, there is only weak evidence for isolation by distance and where it occurs it is associated with drainage basins. In the Pilbara, analyses of genetic structure within localized sub‐locations, just a few kilometres apart, indicate considerable variation between them (FST) and inbreeding within (FIS). We interpret this in the context of cyclonic and other extreme rainfall events that occur episodically, leading to boom‐bust population cycles. Populations decline into refugia of isolated rock patches during extended periods of poor resources with consequent low genetic diversity (He) and large differentiation from others. Populations that irrupt after major episodic rainfall increases resource states, have a more extensive distribution with greater heterozygosity because of gene flow between previously isolated refugia and less differentiation between the locations. These observations have conservation significance for threatened congeneric species and other Australian arid rodents by demonstrating, in this common exemplar, the reduced evolutionary potential imposed by both transient isolation due to climatic variation and longer term disruptions by geographic barriers.