bluuebunny/crossref_metadata_2025_split huggingface dataset:
Flow cytometry-based peripheral blood analysis as an easily friendly tool for prognostic monitoring of acute ischemic stroke: a multicenter study
Authors: Lu Kang , Ni Wanmao , Yue Juanqing , Cheng Yongran , Du Jing , Li Yanchun , Tong Xiangmin , Chen Guo-Bo , Wang Ying 
Published: May 2024

Background and objectiveAcute ischemic stroke (AIS) is a leading cause of mortality, severe neurological and long-term disability world-wide. Blood-based indicators may provide valuable information on identified prognostic factors. However, currently, there is still a lack of peripheral blood indicators for the prognosis of AIS. We aimed to identify the most promising prognostic indicators and establish prognostic models for AIS.Methods484 subjects enrolled from four centers were analyzed immunophenotypic indicators of peripheral blood by flow cytometry. Least absolute shrinkage and selection operator (LASSO) regression was applied to minimize the potential collinearity and over-fitting of variables measured from the same subject and over-fitting of variables. Univariate and multivariable Cox survival analysis of differences between and within cohorts was performed by log-rank test. The areas under the receiving operating characteristic (ROC) curves were used to evaluate the selection accuracy of immunophenotypic indicators in identifying AIS subjects with survival risk. The prognostic model was constructed using a multivariate Cox model, consisting of 402 subjects as a training cohort and 82 subjects as a testing cohort.ResultsIn the prospective study, 7 immunophenotypic indicators of distinct significance were screened out of 72 peripheral blood immunophenotypic indicators by LASSO. In multivariate cox regression, CTL (%) [HR: 1.18, 95% CI: 1.03–1.33], monocytes/μl [HR: 1.13, 95% CI: 1.05–1.21], non-classical monocytes/μl [HR: 1.09, 95% CI: 1.02–1.16] and CD56high NK cells/μl [HR: 1.13, 95% CI: 1.05–1.21] were detected to decrease the survival probability of AIS, while Tregs/μl [HR:0.97, 95% CI: 0.95–0.99, p=0.004], BM/μl [HR:0.90, 95% CI: 0.85–0.95, p=0.023] and CD16+NK cells/μl [HR:0.93, 95% CI: 0.88–0.98, p=0.034] may have the protective effect. As for indicators’ discriminative ability, the AUC for CD56highNK cells/μl attained the highest of 0.912. In stratification analysis, the survival probability for AIS subjects with a higher level of Tregs/μl, BM/μl, CD16+NK cells/μl, or lower levels of CD56highNK cells/μl, CTL (%), non-classical monocytes/μl, Monocytes/μl were more likely to survive after AIS. The multivariate Cox model showed an area under the curve (AUC) of 0.805, 0.781 and 0.819 and 0.961, 0.924 and 0.982 in the training and testing cohort, respectively.ConclusionOur study identified 7 immunophenotypic indicators in peripheral blood may have great clinical significance in monitoring the prognosis of AIS and provide a convenient and valuable predictive model for AIS.
Not seeing the forest for the trees: combination of path integration and landmark cues in human virtual navigation
Authors: Scherer Jonas , Müller Martin M. , Unterbrink Patrick , Meier Sina , Egelhaaf Martin , Bertrand Olivier J. N. , Boeddeker Norbert 
Published: May 2024

IntroductionIn order to successfully move from place to place, our brain often combines sensory inputs from various sources by dynamically weighting spatial cues according to their reliability and relevance for a given task. Two of the most important cues in navigation are the spatial arrangement of landmarks in the environment, and the continuous path integration of travelled distances and changes in direction. Several studies have shown that Bayesian integration of cues provides a good explanation for navigation in environments dominated by small numbers of easily identifiable landmarks. However, it remains largely unclear how cues are combined in more complex environments.MethodsTo investigate how humans process and combine landmarks and path integration in complex environments, we conducted a series of triangle completion experiments in virtual reality, in which we varied the number of landmarks from an open steppe to a dense forest, thus going beyond the spatially simple environments that have been studied in the past. We analysed spatial behaviour at both the population and individual level with linear regression models and developed a computational model, based on maximum likelihood estimation (MLE), to infer the underlying combination of cues.ResultsOverall homing performance was optimal in an environment containing three landmarks arranged around the goal location. With more than three landmarks, individual differences between participants in the use of cues are striking. For some, the addition of landmarks does not worsen their performance, whereas for others it seems to impair their use of landmark information.DiscussionIt appears that navigation success in complex environments depends on the ability to identify the correct clearing around the goal location, suggesting that some participants may not be able to see the forest for the trees.
Single-channel seizure detection with clinical confirmation of seizure locations using CHB-MIT dataset
Authors: Chung Yoon Gi , Cho Anna , Kim Hunmin , Kim Ki Joong 
Published: May 2024

IntroductionLong-term electroencephalography (EEG) monitoring is advised to patients with refractory epilepsy who have a failure of anti-seizure medication and therapy. However, its real-life application is limited mainly due to the use of multiple EEG channels. We proposed a patient-specific deep learning-based single-channel seizure detection approach using the long-term scalp EEG recordings of the Children’s Hospital Boston-Massachusetts Institute of Technology (CHB-MIT) dataset, in conjunction with neurologists’ confirmation of spatial seizure characteristics of individual patients.MethodsWe constructed 18-, 4-, and single-channel seizure detectors for 13 patients. Neurologists selected a specific channel among four channels, two close to the behind-the-ear and two at the forehead for each patient, after reviewing the patient’s distinctive seizure locations with seizure re-annotation.ResultsOur multi- and single-channel detectors achieved an average sensitivity of 97.05–100%, false alarm rate of 0.22–0.40/h, and latency of 2.1–3.4 s for identification of seizures in continuous EEG recordings. The results demonstrated that seizure detection performance of our single-channel approach was comparable to that of our multi-channel ones.DiscussionWe suggest that our single-channel approach in conjunction with clinical designation of the most prominent seizure locations has a high potential for wearable seizure detection on long-term EEG recordings for patients with refractory epilepsy.
Identification of crucial inflammaging related risk factors in multiple sclerosis
Authors: Xu Mengchu , Wang Huize , Ren Siwei , Wang Bing , Yang Wenyan , Lv Ling , Sha Xianzheng , Li Wenya , Wang Yin 
Published: May 2024

BackgroundMultiple sclerosis (MS) is an immune-mediated disease characterized by inflammatory demyelinating lesions in the central nervous system. Studies have shown that the inflammation is vital to both the onset and progression of MS, where aging plays a key role in it. However, the potential mechanisms on how aging-related inflammation (inflammaging) promotes MS have not been fully understood. Therefore, there is an urgent need to integrate the underlying mechanisms between inflammaging and MS, where meaningful prediction models are needed.MethodsFirst, both aging and disease models were developed using machine learning methods, respectively. Then, an integrated inflammaging model was used to identify relative risk factors, by identifying essential “aging-inflammation-disease” triples. Finally, a series of bioinformatics analyses (including network analysis, enrichment analysis, sensitivity analysis, and pan-cancer analysis) were further used to explore the potential mechanisms between inflammaging and MS.ResultsA series of risk factors were identified, such as the protein homeostasis, cellular homeostasis, neurodevelopment and energy metabolism. The inflammaging indices were further validated in different cancer types. Therefore, various risk factors were integrated, and even both the theories of inflammaging and immunosenescence were further confirmed.ConclusionIn conclusion, our study systematically investigated the potential relationships between inflammaging and MS through a series of computational approaches, and could present a novel thought for other aging-related diseases.
Prognostic factors in uterine adenosarcoma: subanalysis of the SARCUT study
Authors: Mancari Rosanna , Yusuf Yildirim , Macuks Ronalds , Achimas-Cadariu Patriciu , Piek Jurgen Martinus , Sperduti Isabella , Corrado Giacomo , Vizza Enrico , Zapardiel Ignacio 
Published: May 2024

ObjectiveThe purpose of the study was to analyse the role of prognostic factors on the risk of recurrence and overall survival of patients with uterine adenosarcoma.MethodsA retrospective international multicentre study involving 46 centres collected 32 cases of uterine adenosarcoma, and these cases were included in the present subanalysis. Clinical and demographic features and tumour characteristics were gathered, as well as information on treatment and relapse. Disease-free and overall survival were analysed.ResultsThe 5-year disease-free survival (DFS) was 85.3% and the 5-year overall survival (OS) rate was 89.5%. The risk factors significantly associated with overall survival were age (HR 1.09, 95% CI 1.03–1.15; p = 0.004) and FIGO stage II–III (HR 17.75, 95% CI 2.87–109.93; p = 0.002). Patients who experienced early relapse (within 12 months) had a tumour size &gt;30 mm and advanced stage. The majority of recurred cases were treated with radiotherapy or surgery and obtained a good response rate.ConclusionThe most significant prognostic factors in uterine adenosarcoma were age and FIGO stage and, indirectly, tumour size at diagnosis. The use of secondary surgery and/or radiotherapy could help in prolonging the disease-free status of the patients.
Association of PD-L1 expression and clinical outcomes in ROS1 - rearranged advanced non-small cell lung cancer treated with crizotinib
Authors: Zhang Huixian , Zhang Ziheng , Yan Ningning , Li Xingya 
Published: May 2024

BackgroundProgrammed cell death ligand 1 (PD-L1) is more readily expressed in ROS proto-oncogene 1 (ROS1) rearranged non-small cell lung cancer (NSCLC) compared to NSCLC cases lacking driver gene mutations. Prior research has established a link between PD-L1 expression and reduced effectiveness of EGFR or ALK inhibitors in EGFR or ALK-positive NSCLC. Nonetheless, the relationship between initial PD-L1 levels and the clinical impact of first-line crizotinib therapy in ROS1-rearranged NSCLC is still uncertain.MethodsFrom January 2016 to December 2021, a total of 246 patients with ROS1 positive tumors were collected. Out of these, 82 patients with advanced ROS1-rearranged NSCLC, who were treated with crizotinib as their initial therapy, were selected for the study. The study aimed primarily to evaluate the objective response rate (ORR) and progression-free survival (PFS), and secondarily to assess disease control rate (DCR) and overall survival (OS).ResultsOf the 82 advanced ROS1-rearranged NSCLC patients, 38 exhibited PD-L1 positivity, subdivided into 11 with high and 27 with low expression levels, while the remaining 44 showed no PD-L1 expression. The ORR for all included patients was 80.5%. No statistically significant variance in ORR was observed among ROS1-rearranged NSCLC patients across differing PD-L1 expression statuses. However, there was a statistically significant difference in DCR between PD-L1 negative group (100%) and high expression group (90.9%) (p=0.04). The median PFS spanned 26.4 months for the PD-L1 negative group, 16.6 for the low expression group, and 13.7 for the high expression group (p=0.001). Additionally, a notable statistical disparity was also observed in median PFS between the PD-L1 negative and positive groups (p=0.02). For the entire study population, the median OS was 53.0 months (95% CI 43.8 - 62.2). In the PD-L1-negative group, the median OS reached 57.2 months, compared to 53.0 months in the PD-L1-positive group, a difference lacking statistical significance (p=0.43).ConclusionsOur results suggest that for ROS1-positive NSCLC patients receiving crizotinib as first-line therapy, PD-L1 expression may serve as a negative prognostic marker for PFS rather than OS.
Investigating Sulforaphane’s anti-virulence and anti-quorum sensing properties against Pseudomonas aeruginosa
Authors: Bendary Mahmoud M. , Ali Mohamed A. M. , Abdel Halim Alyaa S. , Boufahja Fehmi , Chaudhary Anis Ahmad , Elkelish Amr , Soliman Rania H. M. , Hegazy Wael A. H. 
Published: May 2024

BackgroundP. aeruginosa, a significant bacterium, can cause severe illness and resistance to antibiotics. Quorum sensing (QS) systems regulate virulence factors production. Targeting QS could reduce bacteria pathogenicity and prevent antibiotic resistance. Cruciferous vegetables contain sulforaphane, known for its anti-inflammatory, antioxidant, anticancer, and antimicrobial properties.AimWe aimed to examine the inhibitory influences of sulforaphane, at a sub-inhibitory concentration (¼ minimum inhibitory concentration, MIC), on virulence and QS in P. aeruginosa.Materials and methodsThe sulforaphane’s anti-virulence actions at sub-inhibitory concentrations were explored in vitro and in vivo. A sub-MIC concentration of sulforaphane was combined with anti-pseudomonal drugs, and the results of this combination were assessed. The virtual affinity of sulforaphane for the receptors of QS was studied, and its effect on the expression of QS genes was quantified.ResultsSulforaphane significantly decreased the biofilm formation, motility, ability to withstand oxidative stress, and the synthesis of virulence extracellular enzymes such as proteases, hemolysins, and elastase, as well as other virulence factors like pyocyanin. In addition, sulforaphane lessened the severity of P. aeruginosa infection in mice. Sulforaphane reduced the antipseudomonal antibiotics’ MICs when used together, resulting in synergistic effects. The observed anti-virulence impacts were attributed to the ability of sulforaphane to inhibit QS via suppressing the QS genes’ expression.ConclusionSulforaphane shows promise as a potent anti-virulence and anti-QS agent that can be used alongside conventional antimicrobials to manage severe infections effectively. Furthermore, this study paves the way for further investigation of sulforaphane and similar structures as pharmacophores for anti-QS candidates.
The impacts associated with having ADHD: an umbrella review
Authors: French Blandine , Nalbant Gamze , Wright Hannah , Sayal Kapil , Daley David , Groom Madeleine J. , Cassidy Sarah , Hall Charlotte L. 
Published: May 2024

IntroductionAttention Deficit Hyperactivity Disorder (ADHD) affects up to 5% of the population and is characterised by symptoms of impulsivity, hyperactivity and inattention. These symptoms are significantly impairing and carry additional risks for children and adults with ADHD, including negative mental health (e.g. depression), physical health (e.g. obesity) and societal outcomes (e.g. imprisonment, divorce). Very few studies have attempted to synthesise these risks in one publication due to the breadth of evidence published on the adverse outcomes of ADHD.MethodsAn umbrella review was performed to identify reviews (systematic, meta-analysis and narrative) that investigate the risks arising from having ADHD. We conducted a narrative synthesis of the findings and conducted a quality review of the included publications.ResultsUpon searching five databases, 16,675 records were identified. Of these, 125 reviews met the criteria for inclusion. A narrative synthesis of these findings highlighted three key domains of risks associated with ADHD: mental health, physical health, social and lifestyle. Most reviews were of good and moderate quality.DiscussionThis review highlights the many risks associated with having ADHD, beyond its three key symptom domains and the impact of the condition on daily functioning.RegistrationInternational Prospective Register of Systematic Reviews (PROSPERO CRD42023404073).
The Probiotic Strain Lactobacillus acidophilus CL1285 Reduces Fat Deposition and Oxidative Stress and Increases Lifespan in Caenorhabditis elegans
Authors: Bouasker Samir , Nodland Sonja , Millette Mathieu 
Published: May 2024

Caenorhabditis elegans was recently shown to be a powerful model for studying and identifying probiotics with specific functions. Lactobacillus acidophilus CL1285, Lacticaseibacillus casei LBC80R, and Lacticaseibacillus rhamnosus CLR2, which are three bacteria that were marketed by Bio-K+, were evaluated using the nematode C. elegans to study fat accumulation, lifespan, and resistance to oxidative stress. Although the general effects of probiotics in terms of protection against oxidative stress were highlighted, the CL1285 strain had an interesting and specific feature, namely its ability to prevent fat accumulation in nematodes; this effect was verified by both the Oil Red and Nile Red methods. This observed phenotype requires daf-16 and is affected by glucose levels. In addition, in a daf-16- and glucose-dependent manner, CL1285 extended the lifespan of C. elegans; this effect was unique to CL1285 and not found in the other L. acidophilus subtypes in this study. Our findings indicate that L. acidophilus CL1285 impacts fat/glucose metabolism in C. elegans and provides a basis to further study this probiotic, which could have potential health benefits in humans and/or in mammals.
An InGaAs-based Fin-EHBTFET with a heterogate and barrier layer for high performance
Authors: Liu Hu , Li Peifeng , Zhou Xiaoyu , Wang Pengyu , Li Yubin , Pan Lei , Zhang Wenting 
Published: May 2024

Abstract
This paper proposes a fin electron-hole bilayer tunneling FET with a heterogate and an InAlAs barrier layer (HBF-EHBTFET). The heterogate can suppress off-state leakage caused by point tunneling, while the InAlAs barrier layer prevents source–drain direct tunneling, significantly reducing the off-state current (I
off). P-type Gaussian doping can not only solve the problem of the inability to generate a hole layer during device fabrication, but also reduce the turn-on voltage of line-tunneling, ultimately increasing on-state current and reducing average subthreshold swing (SS
avg). By optimizing parameters of the heterogate and InAlAs barrier layer, HBF-EHBTFET can obtain I
off of 2.37 × 10−16 A μm−1, SS
avg of 17.97 mV dec−1, a cutoff frequency (f
T) of 13.2 GHz, and a gain bandwidth product (GBW) of 1.58 GHz. Compared with traditional EHBTFET, HBF-EHBTFET exhibits a reduction in I
off by four orders of magnitude, a decrease in SS
avg by 65.27%, and an increase in f
T and GBW by 78.59% and 93.62%, respectively.
Development of Tm-doped CaHfO3 single-crystal-scintillators for application to integrated-type detectors
Authors: Endo Yusuke , Ichiba Kensei , Nakauchi Daisuke , Kato Takumi , Kawaguchi Noriaki , Yanagida Takayuki 
Published: May 2024

Abstract
Photoluminescence and scintillation properties of Tm:CaHfO3 single crystals were investigated. From the results of photoluminescence properties, all the Tm:CaHfO3 samples showed emission bands due to the 4f–4f transitions of Tm3+, and the 1.0% Tm:CaHfO3 sample showed the highest quantum yield among all the samples upon excitation at 360 nm. The afterglow levels at 20 ms after X-ray irradiation for 2 ms were approximately 12 000 ppm. Pulse height spectra using 137Cs as γ-ray source revealed the 1.0% Tm:CaHfO3 sample showed the highest light yield of 1250 photons MeV−1 among all the samples.
Petroleum Potential Prospectivity of Muglad Basin, South Sudan: A Case of Source Rocks Characterisation in the Drilled Kaikang West-1 Well
Authors: Juach Panom Bol , Joseph Simiyu Wanjala , Gach Gatluok Koang , Rop Bernard Kipsang 
Published: May 2024

This study focuses on Prospectivity of Petroleum Potential of Muglad basin, South Sudan, with the help of petroleum source rocks characterisation in the drilled Kaikang West-1 Well based on the organic matter quantity, quality, and the thermal maturation generation capability of the organic matter disseminated in the analysed rock samples. Muglad basin is a major petroliferous Western and Central African Rifts System (WCARS) member, with estimated reserves of 2,053 mm bbl. Muglad Basin comprises thick sandstones of Aradeiba and Bentiu Formations, considered to be the main petroleum prospectivity targets, with trapping mechanism being structural faults and lithological anticlines. The organic matter contents were determined directly from laboratory analyses of the source rock samples with the help of seismic and gamma ray profiles, total organic carbon (TOC wt %), maturity indices (OI, HI and PI), temperature maximum (Tmax °C), porosities and other sedimentological parameters. This study involved a series of analytical geochemistry and petro-physical studies to ascertain a number of effective source rock samples from the well cores which were then analysed in terms of TOC, OI, PI, HI, S2, S1 and Tmax to determine oil/gas prone samples (resource areas). These were then distinguished from strata with very high organic matter samples as well as very high TOC to help identify for possible source rock characterizations within the lithology pertaining to the well in terms of potential source-reservoir-seals combinations. The Rock-Eval pyrolysis data were useful in assessing and evaluating the type of organic matter, thermal maturity, and the generation capability of source rocks for hydrocarbon exploration rationale. The analyses revealed that some strata, within the sampled well data, have high hydrocarbon generation potential with the existence of commercial hydrocarbon production
Exploring Ethnomathematics on The Batik Patterns of Jember In Mathematics Concept
Authors: Rizqoh Zidna , Anas Aswar , Putra Eric Dwi 
Published: May 2024

This study aims to identify and describe mathematical concepts and describe the results of ethnomathematics exploration contained in Jember batik motifs. The type of research used in this study is qualitative research with an ethnographic approach. The research instruments used were observation, tests, and interviews with batik artisans. The results of this study indicate that the combination of coffee bean and tobacco leaf motifs has applied mathematical concepts of geometry. In the motif of coffee beans and tobacco leaves from the Nhora Pangetu hand-written batik production house, there are mathematical concepts of curves, points, circles, and the concept of dilation. The motif of coffee beans and tobacco leaves from the Gangsar Ngaidin batik production house contains mathematical concepts of reflection (mirroring), congruence, and flat shapes of circles, triangles, and rhombuses.