bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Novel Possible Strategy Based on Self‐Assembly Approach to Achieve Complete Periodontal Regeneration
Authors: Yang Zhen‐Hua , Jin Fang , Zhang Xiao‐Jun , Liu Xin , Zhang Yun‐Fei , Liu Jia‐Qiang , Duan Yin‐Zhong , Jin Yan 
Published: June 2010

AbstractLimitations of current regeneration modalities underscore the importance of restoring the three‐dimensional (3D) microenvironment of periodontal development, which is able to elicit the intrinsic capacity of mesenchymal stem cells to proceed to engage in a redevelopment‐like program. With increased attention for the potential therapeutic applications of periodontal ligament stem cells (PDLSCs) in periodontal regeneration, it has been proposed that bone marrow mesenchymal stem cells (BMMSCs) are very likely another cell source of physiological repair of periodontal tissues. With this in mind, enlightened from the research targeting the fabrication of laminar structures such as liver and kidney with heterotypic stratification of cell sheets, we proposed a novel possible strategy based on self‐assembly approach, which is akin to the physiological phenomenon that occurs during organogenesis, to enhance complete reconstruction of functional complex periodontium‐organ systems. We assumed that in this strategy, using the intrinsic capacity of monodispersed cells to self‐assemble into a microtissue such as a 3D spheroid, bilayered cell pellet constructs comprising calcified bone‐forming cell pellets (i.e., BMMSCs) and cementum/PDL‐forming cell pellets (i.e., PDLSCs) would be fabricated in vitro in a tissue‐mimicking way and then implanted into periodontal defects. We hypothesize that this novel strategy might open new options to reconstruct extended periodontal defects and then achieve the ultimate goal of predictable and complete regeneration of the periodontium.
Proinflammatory Cytokines, Hepatocyte Growth Factor and Adipokines in Peritoneal Dialysis Patients
Authors: Lee Chien‐Te , Ng Hwee‐Yeong , Hsu Chung‐Yao , Tsai Yu‐Che , Yang Yu‐Kun , Chen Te‐Chuan , Chiou Terry Ting‐Yu , Kuo Chien‐Chun , Lee Wen‐Chin , Hsu Kao‐Tai 
Published: June 2010

AbstractChronic inflammation is a well‐recognized complication in dialysis patients and a potential role of the adipose tissue as an important tissue of origin contributing to inflammation has been proposed. Stable peritoneal dialysis (PD) patients were enrolled to investigate the relationship between serum levels of proinflammatory cytokines and adipokines. Our results revealed that there was a strong association between high sensitivity C‐reactive protein and interleukin (IL)‐6 and tumor necrosis factor‐alpha (TNF‐α) but not with IL‐10 and IL‐18. IL‐6 correlated with TNF‐α, IL‐10, and IL‐18. No association was found between IL‐10 and IL‐18. Adiponectin was positively correlated with all proinflammatory cytokines, except IL‐10. No significant association was found between resistin and proinflammatory cytokines. Hepatocyte growth factor (HGF) was directly related to proinflammatory cytokines but not with adipokines. The presence of residual kidney function (RKF) affected IL‐6, TNF‐α, and HGF levels. The peritoneal transport property did not influence inflammatory cytokine and adipokine levels. In conclusion, there was a close relationship between proinflammatory cytokines and adipokines. HGF correlated with proinflammatory cytokines but not with adipokines. The PD‐related factors such as RKF, peritoneal property and dialysis glucose load affected levels of proinflammatory cytokines. Body mass index was an important determinant of leptin and adiponectin in PD patients.
Transcranial Magnetic Stimulation for Migraine: A Safety Review
Authors: Dodick David W. , Schembri Carol T. , Helmuth Michele , Aurora Sheena K. 
Published: June 2010

(Headache 2010;50:1153‐1163)Objective.— To review potential and theoretical safety concerns of transcranial magnetic stimulation (TMS), as obtained from studies of single‐pulse (sTMS) and repetitive TMS (rTMS) and to discuss safety concerns associated with sTMS in the context of its use as a migraine treatment.Methods.— The published literature was reviewed to identify adverse events that have been reported during the use of TMS; to assess its potential effects on brain tissue, the cardiovascular system, hormone levels, cognition and psychomotor tests, and hearing; to identify the risk of seizures associated with TMS; and to identify safety issues associated with its use in patients with attached or implanted electronic equipment or during pregnancy.Results.— Two decades of clinical experience with sTMS have shown it to be a low risk technique with promise in the diagnosis, monitoring, and treatment of neurological and psychiatric disease in adults. Tens of thousands of subjects have undergone TMS for diagnostic, investigative, and therapeutic intervention trial purposes with minimal adverse events or side effects. No discernable evidence exists to suggest that sTMS causes harm to humans. No changes in neurophysiological function have been reported with sTMS use.Conclusions.— The safety of sTMS in clinical practice, including as an acute migraine headache treatment, is supported by biological, empirical, and clinical trial evidence. Single‐pulse TMS may offer a safe nonpharmacologic, nonbehavioral therapeutic approach to the currently prescribed drugs for patients who suffer from migraine.
Headaches After Concussion in US Soldiers Returning From Iraq or Afghanistan
Authors: Theeler Brett J. , Flynn Frederick G. , Erickson Jay C. 
Published: June 2010

(Headache 2010;50:1262‐1272)Objectives.— To determine the prevalence, characteristics, impact, and treatment patterns of headaches after concussion in US Army soldiers returning from a deployment to Iraq or Afghanistan.Methods.— A cross‐sectional study was conducted with a cohort of soldiers undergoing postdeployment evaluation during a 5‐month period at the Madigan Traumatic Brain Injury Program at Ft. Lewis, WA. All soldiers screening positive for a deployment‐related concussion were given a 13‐item headache questionnaire.Results.— A total of 1033 (19.6%) of 5270 returning soldiers met criteria for a deployment‐related concussion. Among those with a concussion, 957 (97.8%) reported having headaches during the final 3 months of deployment. Posttraumatic headaches, defined as headaches beginning within 1 week after a concussion, were present in 361 (37%) soldiers. In total, 58% of posttraumatic headaches were classified as migraine. Posttraumatic headaches had a higher attack frequency than nontraumatic headaches, averaging 10 days per month. Chronic daily headache was present in 27% of soldiers with posttraumatic headache compared with 14% of soldiers with nontraumatic headache. Posttraumatic headaches interfered with duty performance in 37% of cases and caused more sick call visits compared with nontraumatic headache. In total, 78% of soldiers with posttraumatic headache used abortive medications, predominantly over‐the‐counter analgesics, and most perceived medication as effective.Conclusions.— More than 1 in 3 returning military troops who have sustained a deployment‐related concussion have headaches that meet criteria for posttraumatic headache. Migraine is the predominant headache phenotype precipitated by a concussion during military deployment. Compared with headaches not directly attributable to head trauma, posttraumatic headaches are associated with a higher frequency of headache attacks and an increased prevalence of chronic daily headache.
The Shared Genetics of Migraine and Anxious Depression
Authors: Ligthart Lannie , Nyholt Dale R. , Penninx Brenda W.J.H. , Boomsma Dorret I. 
Published: June 2010

(Headache 2010;50:1549‐1560)Objectives.— To investigate (1) whether shared genetic factors influence migraine and anxious depression; (2) whether the genetic architecture of migraine depends on anxious depression; (3) whether the association between migraine and anxious depression is causal.Background.— Migraine and anxious depression frequently occur together, but little is known about the mechanisms causing this association.Methods.— A twin study was conducted to model the genetic architecture of migraine and anxious depression and the covariance between them. Anxious depression was also added to the model as a moderator variable to examine whether anxious depression affects the genetic architecture of migraine. Causal models were explored with the co‐twin control method.Results.— Modest but significant phenotypic (rP = 0.28), genetic (rG = 0.30), and nonshared environmental (rE = 0.26) correlations were found between the 2 traits. Interestingly, the heritability of migraine depended on the level of anxious depression: the higher the anxious depression score, the lower the relative contribution of genetic factors to the individual differences in migraine susceptibility. The observed risk patterns in discordant twins are most consistent with a bidirectional causal relationship.Conclusions.— These findings confirm the genetic association between migraine and anxious depression and are consistent with a syndromic association between the 2 traits. This highlights the importance of taking comorbidity into account in genetic studies of migraine, especially in the context of selection for large‐scale genotyping efforts. Genetic studies may be most effective when migraine with and without comorbid anxious depression are treated as separate phenotypes.
A Survey of Neurologists on the Likeability of Headaches and Other Neurological Disorders
Authors: Evans Randolph W. , Evans Rochelle E. 
Published: June 2010

(Headache 2010;50:1126‐1129)Background.— A pilot survey of 94 neurologists attending a continuing medical education meeting was performed to assess whether neurologists like to treat headaches and other common disorders and evaluate their personal prevalence of the disorders.Methods.— Physicians were asked to respond to the following statement using a 5‐point Likert scale (from 1, strongly disagree to 5, strongly agree): “I like to treat patients with this disease or symptom.”Results.— The response rate was 46% with a mean age of 52.5 years. The respondents liked to treat migraine (mean = 4.32) similarly to carpal tunnel syndrome and Parkinson's disease. Cluster headaches (mean = 3.90) are less liked than migraine similar to epilepsy and multiple sclerosis and respondents are neutral to treating chronic daily headaches (mean = 3.02) similarly to insomnia and low back pain. The lifetime prevalence of migraine among respondents is 48% with those with and without migraine comparably liking to treat migraineurs.Conclusions.— Neurologists like to treat migraine more than cluster headaches and are neutral in treating chronic daily headaches.
Standard‐ versus high‐dose lenograstim in adults with hematologic malignancies for peripheral blood progenitor cell mobilization
Authors: Romeo Azzurra , Chierichini Anna , Spagnoli Alessandra , Vittori Mariangela , Vacca Michele , Gozzer Maria , Spadea Antonio , Anaclerico Barbara , Dessanti Maria Laura , D'Andrea Mariella , Toglia Giuseppe , Annino Luciana , Petti Maria Concetta , Mengarelli Andrea , Arcese William ,   on behalf of the Rome Transplant Network
Published: June 2010

BACKGROUND:The aim of this retrospective, multicenter study was to compare high‐ versus standard‐dose lenograstim after chemotherapy in collecting target dose of CD34+ peripheral blood progenitor cells (PBPCs) in adult candidates for autologous transplant.STUDY DESIGN AND METHODS:A total of 166 consecutive patients (28 acute leukemias [ALs], 77 lymphomas, 61 multiple myeloma [MM]) underwent 182 mobilization procedures. Only the first were analyzed. The CD34+ cell target was at least 2 × 106, 4 × 106, and 8 × 106/kg and lenograstim started on days +19, +1, and +5 from the end of chemotherapy for AL, lymphomas, and MM, respectively. Eighty‐seven and 79 patients, respectively, received 5 and 10 µg/kg/day lenograstim subcutaneously (sc). An analysis to evaluate factors predicting satisfactory procedures and outcome of transplants performed with first‐mobilization‐procedure PBPCs was conducted. Most patients received 6 mg of pegfilgrastim or 5 µg/kg/day lenograstim sc after transplant.RESULTS:In multivariate analysis, high‐dose lenograstim (p = 0.053) in MM and male sex (p = 0.028) were positive predictive factors for reaching cell target. Fludarabine negatively influenced stimulation length (p = 0.002). Apheresis, CD34+ cells mobilized and collected, blood volume processed, side effects, transplants performed, and engraftment time were similar between lenograstim cohorts. Pegfilgrastim versus lenograstim delayed platelet (PLT) recovery times (13 days vs. 11 days, p = 0.036).CONCLUSIONS:High‐dose lenograstim more efficiently mobilized MM patients requiring the highest PBPC target but did not influence transplants performed and engraftment time. Male patients mobilized more efficiently. Fludarabine negatively influenced stimulation length. Finally, pegfilgrastim seems to delay PLT recovery.
Modeling the risk of an emerging pathogen entering the Canadian blood supply
Authors: Kleinman Steven , Cameron Claire , Custer Brian , Busch Michael , Katz Louis , Kralj Boris , Matheson Ian , Murphy Ken , Preiksaitis Jutta , Devine Dana 
Published: June 2010

BACKGROUND: As part of its risk management process, Canadian Blood Services (CBS) constructed mathematical models of how newly emerging pathogens might affect blood transfusion recipients.STUDY DESIGN AND METHODS: CBS convened an expert panel including medical, health economics, analytical, risk management, and insurance professionals to examine multiple data sources. The model for emerging pathogen risk included separate modules to calculate the frequency and severity of infections from transfusion‐transmitted agents that could cause either acute transient or chronic persistent infection. Important model input variables were annual number of components transfused, the presumed incidence and prevalence of a new agent, the time interval of recipient risk, recipient age and sex, projected recipient survival, rate of secondary infection, pathogen‐induced morbidity, and the associated medical costs of such morbidity.RESULTS: In the 5‐year time frame considered in the model, it was estimated that approximately 3500 recipient infections (two‐SD range of 0 to 11,370 infections) could occur from an emerging pathogen that establishes a chronic infection in donors, with 60% of these due to red blood cell transfusion. The medical costs associated with recipient outcomes due to a catastrophic emerging pathogen could be lowered by 20% if an effective pathogen reduction method for either platelets or plasma were in place.CONCLUSION: This modeling exercise offers a framework for other blood services to construct similar models. It also provides a useful way to model the implementation of new blood safety interventions (e.g., pathogen reduction) on emerging pathogen risk.
National survey of hepatitis B virus (HBV) polymorphism in asymptomatic HBV blood donors from 1999 to 2007 in France
Authors: Servant‐Delmas Annabelle , Mercier Mélanie , El Ghouzzi Marie‐Hélène , Girault Annie , Bouchardeau Françoise , Pillonel Josiane , Laperche Syria 
Published: June 2010

BACKGROUND: Hepatitis B virus (HBV) diversity is characterized by eight genotypes correlated to eight hepatitis B surface antigen (HBsAg) subtypes, which differ in their geographical distribution.STUDY DESIGN AND METHODS: To establish virologic characteristics and the evolution of HBV diversity, we carried out a study over a 9‐year period in HBV‐infected French blood donors. HBsAg subtyping based on specific antibody method concerned 2901 donors, from whom 940 have been analyzed by an S‐gene sequencing to determine genotypes and S‐gene mutations.RESULTS: HBsAg subtypes were distributed as follows: ayw2, 34.4%; adw2, 25.7%; ayw1, 10.2%; ayw4, 14.9%; adr, 7.8%; ayw3, 6.4%; and adw4, 0.7%. Ayw4 (Genotype E) proportion increased over time in correlation with an increased proportion of subjects originated from sub‐Saharan Africa. The genotype observed with the highest proportion was D (43.0%), then A (26.2%), E (17.5%), B (6.5%), C (6.4%), and F (0.4%). Genotype B had the highest proportion of hepatitis B e antigen (39.2%) and the highest viral loads (VLs). Forty‐three (5.5%) isolates presented one (n = 35) or multiple (n = 8) amino acid envelope substitutions. Donors infected with mutated isolates had lowest VLs. rtA181T/sW172 stop mutation associated with resistance to nucleos(t)ide analogs was detected in two donors suggesting a transmission of these isolates.CONCLUSION: This extensive study shows that HBV genotype evolution is closely linked to the geographical origin of subjects and that the occurrence of viral envelope mutants is not an exceptional event in healthy HBV chronic carriers. Blood donors rarely recruited in HBV studies provide further relevant information on the characteristics of HBV diversity.
Effect of platelet additive solution on bacterial dynamics and their influence on platelet quality in stored platelet concentrates
Authors: Greco Carey A. , Zhang Jerry G. , Kalab Miloslav , Yi Qi‐Long , Ramirez‐Arcos Sandra M. , Gyongyossy‐Issa Maria I.C. 
Published: June 2010

BACKGROUND: Platelet additive solutions (PASs) are an alternative to plasma for the storage of platelet concentrates (PCs). However, little is known about the effect of PAS on the growth dynamics of contaminant bacteria. Conversely, there have been no studies on the influence of bacteria on platelet (PLT) quality indicators when suspended in PAS.STUDY DESIGN AND METHODS: Eight buffy coats were pooled, split, and processed into PCs suspended in either plasma or PAS (SSP+, MacoPharma). PCs were inoculated with 10 and 100 colony‐forming units (CFUs)/bag of either Serratia liquefaciens or Staphylococcus epidermidis. Bacterial growth was measured over 5 days by colony counts and bacterial biofilm formation was assayed by scanning electron microscopy and crystal violet staining. Concurrently, PLT markers were measured by an assay panel and flow cytometry.RESULTS: S. liquefaciens exhibited an apparent slower doubling time in plasma‐suspended PCs (plasma‐PCs). Biofilm formation by S. liquefaciens and S. epidermidis was significantly greater in PCs stored in plasma than in PAS. Although S. liquefaciens altered several PLT quality markers by Days 3 to 4 postinoculation in both PAS‐ and plasma‐PCs, S. epidermidis contamination did not produce measurable PLT changes.CONCLUSIONS: S. liquefaciens can be detected more quickly in PAS‐suspended PCs (PAS‐PCs) than in plasma‐PCs by colony counting. Furthermore, reduced biofilm formation by S. liquefaciens and S. epidermidis during storage in PAS‐PCs increases bacteria availability for sampling detection. Culture‐based detection remains the earliest indicator of bacterial presence in PAS‐PCs, while changes of PLT quality can herald S. liquefaciens contamination when in excess of 108 CFUs/mL.
Multiple pH measurement during storage may detect bacterially contaminated platelet concentrates
Authors: Barker Lynn M. , Nanassy Oliver Z. , Reed Michael W. , Geelhood Steven J. , Pfalzgraf Randy D. , Cangelosi Gerard A. , De Korte Dirk 
Published: June 2010

BACKGROUND: Bacterial contamination or platelet (PLT) metabolism can change the pH of stored PLT concentrates (PCs). Measurement of pH for quality control is currently done on a limited basis. An easy noninvasive method was developed to obtain sequential pH measurements over time, without risking contamination and/or consuming PCs.STUDY DESIGN AND METHODS: The objective was to measure pH profiles of bacterially contaminated PCs over 7 days of storage. Small‐volume PC storage bags with incorporated pH sensor were prepared and in vitro variables were tested using aliquots of PCs. The pH sensors were used to delineate trends associated with the deterioration of these PCs upon inoculation with 19 different bacterial strains and one yeast.RESULTS: Monitoring the pH trends in real time in a noninvasive fashion, most bacterial strains were detected within 24 to 72 hours after spiking into the bag. At the time of detection, bacterial concentrations had reached levels between 1 × 103 and 1 × 108 colony‐forming units/mL. Several strains had pH rebound after initial drop. Multiple noninvasive pH reads allowed bacterial detection whereas single pH reads could give false‐negative results.CONCLUSIONS: The noninvasive pH sensor facilitated the detection of most strains of bacterial contaminants within 3 days with no potential for sampling error.
Evaluation of partial sterility in mating performance and reproduction of the West Indian sweetpotato weevil, Euscepes postfasciatus
Authors: Kumano Norikuni , Kuriwada Takashi , Shiromoto Keiko , Haraguchi Dai , Kohama Tsuguo 
Published: June 2010

AbstractThe sterile insect technique (SIT) is based on population and behavioral ecology and is widely used to suppress or eradicate target pest insect populations. The effectiveness of SIT depends on the ability of the released sterile males to mate with and inseminate wild females. The use of gamma‐radiation to induce sterility is, however, associated with negative impacts not only on reproductive cells but also on somatic cells. Consequently, irradiation for sterilization diminishes mating performance over time. In this study, we evaluated the balance between the irradiation dose and both fertility and mating propensity in Euscepes postfasciatus (Fairmaire) (Coleoptera: Curculionidae) for 22 days following irradiation. The mating propensity of males irradiated with a 150‐Gy dose, as currently used to induce complete sterility of E. postfasciatus in the SIT program in Okinawa Prefecture, was equal to that of non‐irradiated weevils for up to 6 days, and the mating propensity of males irradiated with a dose of 125 Gy was equal to that of non‐irradiated weevils for twice this period (12 days). The fertilization ability of weevils irradiated with a dose of 125 Gy was reduced by 4.6% in males and 0.6% in females, compared to the potential fertilization ability. We also discuss the possibility of the application of partially sterilized insects in eradication programs.
Elastin in oral connective tissue modulates the keratinization of overlying epithelium
Authors: Hsieh Po‐Chen , Jin Ying‐Tai , Chang Chia‐Wen , Huang Chi‐Chou , Liao Shih‐Chung , Yuan Kuo 
Published: June 2010

Hsieh P‐C, Jin Y‐T, Chang C‐W, Huang C‐C, Liao S‐C, Yuan K. Elastin in oral connective tissue modulates the keratinization of overlying epithelium. J Clin Periodontol 2010; 37: 705‐711 doi: 10.1111/j.1600-051X.2010.01542.xAbstractAim: One of the most discernable differences between keratinized and non‐keratinized oral mucosas is the quantity of elastin they contain in the connective tissues. Whether elastin modulates the keratin expression of oral epithelial cells is unknown.Methods: Four specimens containing both keratinized and non‐keratinized mucosas were processed for immunohistochemical (IHC) stainings for elastin and four keratins. Six keratinized and non‐keratinized portions of oral mucosas were dissected and cultured on an organ culture system. Purified elastin and elastase were added separately to the media. After 14 days, the mucosas were examined for four keratin expressions. Cell cultures of keratinized and non‐keratinized gingival fibroblasts were established and tested for elastin expression. Oral mucosa equivalents were then engineered and tested for keratin expression.Results: Keratinized epithelium exclusively expressed keratin‐1 and ‐10 (K1/10), while non‐keratinized epithelium expressed keratin‐4 and ‐13 (K4/13). Only non‐keratinized fibroblasts expressed elastin in cell culture. Both the native and the engineered keratinized gingiva changed phenotypes and expressed K4/13 when treated with exogenous elastin. On the contrary, the native non‐keratinized mucosa started to express K1/10 when elastase eradicated inherent elastin.Conclusions: Our study demonstrated that the elastin in the oral connective tissue is important for the non‐keratinized phenotypes of overlaying epithelium.