bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modified pulsed continuous arterial spin labeling for labeling of a single artery
Authors: Dai Weiying , Robson Philip M. , Shankaranarayanan Ajit , Alsop David C. 
Published: July 2010

AbstractImaging the contribution of different arterial vessels to the blood supply of the brain can potentially guide the treatment of vascular disease and other disorders. Previously available only with catheter angiography, vessel‐selective labeling of arteries has now been demonstrated with pulsed and continuous arterial spin labeling methods. Pulsed continuous labeling, which permits continuous labeling on standard scanner radiofrequency hardware, has been used to encode the contribution of different vessels to the blood supply of the brain. Vessel encoding requires a longer scan and a more complex reconstruction algorithm and may be more sensitive to fluctuations in flow, however. Here a method is presented for single‐artery selective labeling, in which a disk around the targeted vessel is labeled. Based on pulsed continuous labeling, this method is achieved by rotating the directions of added in‐plane gradients. Numerical simulations of the simplest strategy show good efficiency but poor suppression of labeling at large distances from the target vessel. Amplitude modulation of the rotating in‐plane gradients results in better suppression of distant vessels. In vivo results demonstrate highly selective labeling of individual vessels and a rapid falloff of the labeling with distance from the center of the labeling disk, in agreement with the simulations. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.
Quantitative magnetization transfer in in vivo healthy human skeletal muscle at 3 T
Authors: Sinclair Christopher D. J. , Samson Rebecca S. , Thomas David L. , Weiskopf Nikolaus , Lutti Antoine , Thornton John S. , Golay Xavier 
Published: July 2010

AbstractThe value of quantitative MR methods as potential biomarkers in neuromuscular disease is being increasingly recognized. Previous studies of the magnetization transfer ratio have demonstrated sensitivity to muscle disease. The aim of this work was to investigate quantitative magnetization transfer imaging of skeletal muscle in healthy subjects at 3 T to evaluate its potential use in pathological muscle. The lower limb of 10 subjects was imaged using a 3D fast low‐angle shot acquisition with variable magnetization transfer saturation pulse frequencies and amplitudes. The data were analyzed with an established quantitative two‐pool model of magnetization transfer. T1 and B1 amplitude of excitation radiofrequency field maps were acquired and used as inputs to the quantitative magnetization transfer model, allowing properties of the free and restricted proton pools in muscle to be evaluated in seven different muscles in a region of interest analysis. The average restricted pool T2 relaxation time was found to be 5.9 ± 0.2μs in the soleus muscle and the restricted proton pool fraction was 8 ± 1%. Quantitative magnetization transfer imaging of muscle offers potential new biomarkers in muscle disease within a clinically feasible scan time. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.
Facioscapulohumeral muscular dystrophy presenting with isolated axial myopathy and bent spine syndrome
Authors: Kottlors Michael , Kress Wolfram , Meng Gerhard , Glocker Franz X. 
Published: July 2010

AbstractSeveral subtypes of facioscapulohumeral muscular dystrophy (FSHD) with atypical clinical presentation have been described. We report a new, distinct phenotype with progressive bent spine syndrome solely affecting the paraspinal muscles. Magnetic resonance imaging study of the lumbar spine revealed marked atrophy of the paraspinal muscles. The diagnosis was confirmed by DNA testing, which revealed shortened restriction fragments of the D4Z4 repeat on haplotype A in connection with a positive family history. Muscle Nerve 42:273–275, 2010
Utility of electrodiagnostic testing in evaluating patients with lumbosacral radiculopathy: An evidence‐based review
Authors: Cho S. Charles , Ferrante Mark A. , Levin Kerry H. , Harmon Robert L. , So Yuen T. 
Published: July 2010

AbstractThis is an evidence‐based review of electrodiagnostic (EDX) testing of patients with suspected lumbosacral radiculopathy to determine its utility in diagnosis and prognosis. Literature searches were performed to identify articles applying EDX techniques to patients with suspected lumbosacral radiculopathy. From the 355 articles initially discovered, 119 articles describing nerve conduction studies, electromyography (EMG), or evoked potentials in adequate detail were reviewed further. Fifty‐three studies met inclusion criteria and were graded using predetermined criteria for classification of evidence for diagnostic studies. Two class II, 7 class III, and 34 class IV studies described the diagnostic use of EDX. One class II and three class III articles described H‐reflexes with acceptable statistical significance for use in the diagnosis and confirmation of suspected S1 lumbosacral radiculopathy. Two class II and two class III studies demonstrated a range of sensitivities for use of muscle paraspinal mapping. Two class II studies demonstrated the utility of peripheral myotomal limb electromyography in radiculopathies. Muscle Nerve 42:276–282, 2010
A ‘new’ palaeolithic dog from central Europe
Authors: Napierala Hannes , Uerpmann Hans‐Peter 
Published: July 2010

AbstractKesslerloch Cave in Switzerland is one of the major Magdalenian sites in Central Europe. During a recent reanalysis of the faunal remains, we identified a cranial fragment and teeth of the domestic dog. The large maxillar fragment was directly dated to 12.225±45 bp (KIA‐33350) or c. 14.100‐14.600 BP. The finds are metrically well below the natural variability of wolves from both Palaeolithic and recent times and even show slight morphological differences to the wild wolves from the site. We argue that the maxilla fragment must now be considered the earliest indisputable directly dated evidence of a domestic dog. Copyright © 2010 John Wiley & Sons, Ltd.
Mechanism of charge recombination and IPCE in ZnO dye‐sensitized solar cells having I−/I and Br−/Br redox couple
Authors: Rani Seema , Suri Poonam , Mehra Ram Mohan 
Published: July 2010

AbstractThe influence of intensity and wavelength variation on the solar cell parameters of two different ZnO‐based liquid state DSSCs named as Cell (A) ZnO/EosinY/LiI and Cell (B) ZnO/EosinY/LiBr was studied. It was found that Voc and Isc depend logarithmically and linearly on light flux, respectively, which indicates that light absorption and carrier diffusion do not limit the solar cell efficiency. The data was analyzed to ascertain the charge recombination mechanism between conduction band electrons and the electrolytes. The regeneration of dye due to I−/I3 and Br−/Br redox couple was examined by studying the wavelength dependence of IPCE. An estimation of series and shunt resistance is made using two methods: (i) different illumination method (ii) single I–V curve, for the two cells in order to understand the role of the electrolyte in controlling the solar cell parameters. Copyright © 2010 John Wiley & Sons, Ltd.
High efficiency screen printed bifacial solar cells on monocrystalline CZ silicon
Authors: Yang L. , Ye Q.H. , Ebong A. , Song W.T. , Zhang G.J. , Wang J.X. , Ma Y. 
Published: July 2010

AbstractWe present industrialized bifacial solar cells on large area (149 cm2) 2 cm CZ monocrystalline silicon wafers processed with industrially relevant techniques such as liquid source BBr3 and POCl3 open‐tube furnace diffusions, plasma enhanced chemical vapor deposition (PECVD) SiNx deposition, and screen printed contacts. The fundamental analysis of the paste using at boron‐diffused surface and the bifacial solar cell firing cycle has been investigated. The resulting solar cells have front and rear efficiencies of 16.6 and 12.8%, respectively. The ratio of the rear JSC to front JSC is 76.8%. It increases the bifacial power by 15.4% over a conventional solar cell at 20% of 1‐sun rear illumination, which equals to the power of a conventional solar cell with 19.2% efficiency. We also present a bifacial glass–glass photovoltaic (PV) module with 30 bifacial cells with the electrical characteristics. Copyright © 2010 John Wiley & Sons, Ltd.
A gel‐free quantitative proteomics approach to investigate temperature adaptation of the food‐borne pathogen Cronobacter turicensis 3032
Authors: Carranza Paula , Grunau Alexander , Schneider Thomas , Hartmann Isabel , Lehner Angelika , Stephan Roger , Gehrig Peter , Grossmann Jonas , Groebel Katrin , Hoelzle Ludwig E. , Eberl Leo , Riedel Kathrin 
Published: July 2010

AbstractThe opportunistic food‐borne pathogen Cronobacter sp. causes rare but significant illness in neonates and is capable to grow at a remarkably wide range of temperatures from 5.5 to 47°C. A gel‐free quantitative proteomics approach was employed to investigate the molecular basis of the Cronobacter sp. adaptation to heat and cold‐stress. To this end the model strain Cronobacter turicensis 3032 was grown at 25, 37, 44, and 47°C, and whole‐cell and secreted proteins were iTRAQ‐labelled and identified/quantified by 2‐D‐LC‐MALDI‐TOF/TOF‐MS. While 44°C caused only minor changes in C. turicensis growth rate and protein profile, 47°C affected the expression of about 20% of all 891 identified proteins and resulted in a reduced growth rate and rendered the strain non‐motile and filamentous. Among the heat‐induced proteins were heat shock factors, transcriptional and translational proteins, whereas proteins affecting cellular morphology, proteins involved in motility, central metabolism and energy production were down‐regulated. Notably, numerous potential virulence factors were found to be up‐regulated at higher temperatures, suggesting an elevated pathogenic potential of Cronobacter sp. under these growth conditions. Significant alterations in the protein expression profile and growth rate of C. turicensis exposed to 25°C indicate that at this temperature the organism is cold‐stressed. Up‐regulated gene products comprised cold‐shock, DNA‐binding and ribosomal proteins, factors that support protein folding and proteins opposing cold‐induced decrease in membrane fluidity, whereas down‐regulated proteins were mainly involved in central metabolism.
Towards a two‐dimensional proteomic reference map of Bradyrhizobium japonicum CPAC 15: Spotlighting “hypothetical proteins”
Authors: da Silva Batista Jesiane Stefânia , Torres Adalgisa Ribeiro , Hungria Mariangela 
Published: July 2010

AbstractThe economic and ecological importance of the symbiosis of soybean with Bradyrhizobium japonicum strains is significant in several countries, particularly Brazil; however, up to now, only one complete and a draft genome for this species are available. In this study, we have obtained a proteomic reference map of B. japonicum strain CPAC 15 (=SEMIA 5079) – used in commercial inoculants for application to soybean crops in Brazil – grown under in vitro conditions. CPAC 15 belongs to the same serogroup as strain USDA 123, and both are known as the soybean bradyrhizobial strains with highest competitive and saprophytic known so far. To increase the precision of the proteomic map, we compared whole‐cell 2‐D protein gel‐electrophoresis profiles of CPAC 15 and of two related strains. One‐hundred and seventy representative spots, selected from the three profiles, were analyzed by MS. In total, 148 spots were successfully identified as cytoplasmic and periplasmic proteins belonging to diverse metabolic pathways, several of them related to the saprophytic and competitive abilities of CPAC 15. We attributed probable functions to 26 hypothetical proteins, including those involved in polyhydroxybutyrate metabolism, β‐lactamase, stress responses and aromatic compound degradation, all with high probability of being related to the saprophytic ability of CPAC 15. In addition, by providing valuable information about expressed proteins in B. japonicum in vitro, our results emphasize the importance of accurate functional annotation of uncharacterized expressed proteins, improving considerably our understanding of the legume–rhizobia symbiosis.
Creation of an antibody‐based subcellular protein atlas
Authors: Lundberg Emma , Uhlén Mathias 
Published: July 2010

AbstractAn important part for understanding the complex machinery of living cells is to know the spatial distribution of proteins all the way from organ to organelle levels. An equally important part of proteomics is to map the subcellular distribution of all human proteins. Here, we discuss methodologies for systematic subcellular profiling with emphasis on the antibody‐based approach performed as a part of the Human Protein Atlas project. The considerations made when creating the subcellular protein atlas and critical parameters of this approach are discussed.
Functional genomic profiling of Aspergillus fumigatus biofilm reveals enhanced production of the mycotoxin gliotoxin
Authors: Bruns Sandra , Seidler Marc , Albrecht Daniela , Salvenmoser Stefanie , Remme Nicole , Hertweck Christian , Brakhage Axel A. , Kniemeyer Olaf , Müller Frank‐Michael C. 
Published: July 2010

AbstractThe opportunistic pathogenic mold Aspergillus fumigatus is an increasing cause of morbidity and mortality in immunocompromized and in part immunocompetent patients. A. fumigatus can grow in multicellular communities by the formation of a hyphal network encased in an extracellular matrix. Here, we describe the proteome and transcriptome of planktonic‐ and biofilm‐grown A. fumigatus mycelium after 24 and 48 h. A biofilm‐ and time‐dependent regulation of many proteins and genes of the primary metabolism indicates a developmental stage of the young biofilm at 24 h, which demands energy. At a matured biofilm phase, metabolic activity seems to be reduced. However, genes, which code for hydrophobins, and proteins involved in the biosynthesis of secondary metabolites were significantly upregulated. In particular, proteins of the gliotoxin secondary metabolite gene cluster were induced in biofilm cultures. This was confirmed by real‐time PCR and by detection of this immunologically active mycotoxin in culture supernatants using HPLC analysis. The enhanced production of gliotoxin by in vitro formed biofilms reported here may also play a significant role under in vivo conditions. It may confer A. fumigatus protection from the host immune system and also enable its survival and persistence in chronic lung infections such as aspergilloma.
Analysis conditions for proteomic profiling of mammalian tissue and cell extracts with antibody microarrays
Authors: Alhamdani Mohamed Saiel Saeed , Schröder Christoph , Hoheisel Jörg D. 
Published: July 2010

AbstractAntibody microarrays are a developing tool for global proteomic profiling. A protocol was established that permits robust analyses of protein extracts from mammalian tissues and cells rather than body fluids. The factors optimized were buffer composition for surface blocking, blocking duration, protein handling and processing, labeling parameters like type of dye, molar ratio of label versus protein, and dye removal, as well as incubation parameters such as duration, temperature, buffer, and sample agitation.
Nucleolar proteomics and viral infection
Authors: Hiscox Julian A. , Whitehouse Adrian , Matthews David A. 
Published: July 2010

AbstractRecent advances in proteomics have been combined with traditional methods for isolation of nucleoli from mammalian and plant cells. This approach has confirmed the growing body of data showing a wide role for the nucleolus in eukaryotic cell biology beyond ribosome generation into many areas of cell function from regulation of the cell cycle, modulation of the cell stress response to innate immune responses. This has been reflected in the growing body of evidence that viruses specifically target the nucleolus by sequestering cellular nucleolar proteins or by targeting viral proteins to the nucleolus in order to maximise viral replication. This review covers those key areas and looks at the latest approaches using high‐throughput quantitative proteomics of the nucleolus in virus infected cells to gain an insight into the role of this fascinating compartment in viral infection.
Evaluation of Plasma Modified Polycaprolactone Honeycomb Scaffolds by Human Mesenchymal Stem Cells Cultured in Vitamin D Differentiation Medium
Authors: Formosa Fabio , Sánchez‐Vaquero Vanessa , Rodríguez‐Navas Carmen , Muñoz‐Noval Álvaro , Tejera‐Sanchez Nuria , Silván Miguel Manso , García‐Ruiz Josefa Predestinación , Marletta Giovanni 
Published: July 2010

AbstractPolycaprolactone (PCL) scaffolds with a honeycomb (hc) microstructure have been prepared, modified at surface level by plasma processes and evaluated with human mesenchymal stem cells (hMSCs). Thin scaffolds were fabricated by self‐assembly of PCL in the presence of an amphiphilic copolymer under controlled humidity. The surface of the so formed PCLhc scaffolds was modified by Ar and ArO2plasma discharges and by plasma deposition of allylamine (ALA). Such modification induces composition changes and microstructure modifications as determined by Fourier transformed infrared spectroscopy and scanning electron microscopy. As a consequence of these treatments, the hydrophobic character of PCLhc scaffolds is rectified as derived from water contact angle measurements. The hMSC cytocompatibility of these scaffolds was initially assayed under proliferation conditions evaluating surface–cell interactions by fluorescence and confocal microscopy. Most relevant behaviour was observed for hMSCs cultured onto ALA modified scaffolds in view of the cytoskeleton polarization. These modified surfaces were further explored for differentiation of hMSCs in the presence of vitamin D, giving rise to secreting vesicles and increased extension of the cytoskeleton. These results are promising for the development of cell monolayer tissue regenerating platforms.magnified image
The Development of a New Synthesis Process for 3,3′‐Diamino‐4,4′‐azoxyfurazan (DAAF)
Authors: Francois Elizabeth G. , Chavez David E. , Sandstrom Mary M. 
Published: July 2010

AbstractProcess optimization studies were performed for the preparation of the high explosive 3,3′‐diamino‐4,4′‐azoxyfurazan (DAAF). These process studies were pursued to address issues such as problematic waste generation products, particle size, impurities, and manufacturability. This paper describes the original synthesis method and inherent issues. An optimization process was designed to investigate the issues with purity and manufacturability. Particle size effects were addressed by adding a recrystallization step to the synthesis. Ultimately, a complete solution to all observed issues was found with a new synthesis process, which now allows DAAF to be prepared without any impurities, with good particle size and without the need for recrystallization. Importantly, the new synthesis process can be performed in an environmentally friendly manner, with the production of non‐hazardous waste.
Green building project management: obstacles and solutions for sustainable development
Authors: Hwang Bon‐Gang , Tan Jac See 
Published: July 2010

ABSTRACTGreen building construction is earning a place in Singapore's construction industry, and, with augmenting cognizance of environmental issues and growing concern over climate change, sustainable construction is gradually being put forth globally. However, construction of green buildings in Singapore still encounters impediments, as there is a lack of proper project management framework for such projects. Based on survey and interview results from 31 industry experts, this study aims to identify common obstacles encountered during management of green construction projects, ultimately proposing some solutions to overcome the barriers. The findings from this study reveal that, although project cost is the paramount barrier among others in green building construction management, there is no paucity in sustainable knowledge in Singapore's construction industry. To deal with the cost related problem, the coverage of government incentives should be widened to include the usage of green products and technologies. Furthermore, a project management framework for green building construction should be developed to overcome the barriers, possibly promoting adoption of sustainable construction in future projects. Copyright © 2010 John Wiley & Sons, Ltd and ERP Environment.
Assessment of the ductus venosus, tricuspid blood flow and the nasal bone in second‐trimester screening for trisomy 21
Authors: Stressig R. , Kozlowski P. , Froehlich S. , Siegmann H. J. , Hammer R. , Blumenstock G. , Kagan K. O. 
Published: July 2010

AbstractObjectiveTo examine the prevalence of reversed a‐wave in the ductus venosus, tricuspid regurgitation and absent nasal bone, in a second‐trimester population undergoing amniocentesis, after exclusion of major fetal defects and to estimate the performance in screening for trisomy 21 based on maternal age and these markers in a general population.MethodsThis was a retrospective study involving pregnancies undergoing amniocentesis due to increased risk for trisomy 21, mainly because of advanced maternal age. Before the invasive procedure, an ultrasound examination was carried out to exclude major fetal defects and to examine the ductus venosus, tricuspid blood flow and the presence of the fetal nasal bone. Modeling techniques were used based on 20 000 euploid pregnancies and 20 000 pregnancies with trisomy 21 to assess the screening performance in a general population.ResultsThe study population consisted of 3613 euploid pregnancies and 35 cases with trisomy 21. In the euploid group, reversed flow in the ductus venosus, tricuspid regurgitation and an absent nasal bone was observed in 1.7%, 1.5% and 0.1% of cases, respectively. In the trisomic group, these markers were found in 14.3%, 11.4% and 14.3% of cases, respectively. For a 5% false‐positive rate, the detection rate in screening for trisomy 21, based on maternal age and either ductus venosus, tricuspid blood flow or nasal bone would be 33.8%, 32.4% or 31.4%, respectively. Screening by maternal age alone would detect 29.0% of the fetuses with trisomy 21. Receiver–operating characteristics curve analysis showed a slight but significant improvement in screening performance for trisomy 21 based on the inclusion of these markers.ConclusionSecond‐trimester ultrasound screening for trisomy 21 based on maternal age with additional assessment of the ductus venosus, tricuspid blood flow and the fetal nasal bone in otherwise normal‐appearing fetuses is only marginally better than is screening by maternal age alone. Copyright © 2011 ISUOG. Published by John Wiley & Sons, Ltd.