bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of mammographic density with hormone receptors in invasive breast cancers: Results from a case‐only study
Authors: Heusinger Katharina , Jud Sebastian M. , Häberle Lothar , Hack Carolin C. , Adamietz Boris R. , Meier‐Meitinger Martina , Lux Michael P. , Wittenberg Thomas , Wagner Florian , Loehberg Christian R. , Uder Michael , Hartmann Arndt , Schulz‐Wendtland Rüdiger , Beckmann Matthias W. , Fasching Peter A. 
Published: March 2012

AbstractFor many breast cancer (BC) risk factors, there is growing evidence concerning molecular subtypes for which the risk factor is specific. With regard to mammographic density (MD), there are inconsistent data concerning its association with estrogen receptor (ER) and progesterone receptor (PR) expression. The aim of our study was to analyze the association between ER and PR expression and MD. In our case‐only study, data on BC risk factors, hormone receptor expression and MD were available for 2,410 patients with incident BC. MD was assessed as percent MD (PMD) using a semiautomated method by two readers for every patient. The association of ER/PR and PMD was studied with multifactorial analyses of covariance with PMD as the target variable and including well‐known factors that are also associated with MD, such as age, parity, use of hormone replacement therapy, and body mass index (BMI). In addition to the commonly known associations between PMD and age, parity, BMI and hormone replacement therapy, a significant inverse association was found between PMD and ER expression levels. Patients with ER‐negative tumors had an average PMD of 38%, whereas patients with high ER expression had a PMD of 35%. A statistical trend toward a positive association between PMD and PR expression was also seen. PMD appears to be inversely associated with ER expression and may correlate positively with PR expression. These effects were independent of other risk factors such as age, BMI, parity, and hormone replacement therapy, possibly suggesting other pathways that mediate this effect.
Variety in vegetable and fruit consumption and the risk of gastric and esophageal cancer in the European prospective investigation into cancer and nutrition
Authors: Jeurnink S.M. , Büchner F.L. , Bueno‐de‐Mesquita H.B. , Siersema P.D. , Boshuizen H.C. , Numans M.E. , Dahm C.C. , Overvad K. , Tjønneland A. , Roswall N. , Clavel‐Chapelon F. , Boutron‐Ruault M.C. , Morois S. , Kaaks R. , Teucher B. , Boeing H. , Buijsse B. , Trichopoulou A. , Benetou V. , Zylis D. , Palli D. , Sieri S. , Vineis P. , Tumino R. , Panico S. , Ocké M.C. , Peeters P.H.M. , Skeie G. , Brustad M. , Lund E. , Sánchez‐Cantalejo E. , Navarro C. , Amiano P. , Ardanaz E. , Ramón Quirós J. , Hallmans G. , Johansson I. , Lindkvist B. , Regnér S. , Khaw K.T. , Wareham N. , Key T.J. , Slimani N. , Norat T. , Vergnaud A.C. , Romaguera D. , Gonzalez C.A. 
Published: March 2012

AbstractDiets high in vegetables and fruits have been suggested to be inversely associated with risk of gastric cancer. However, the evidence of the effect of variety of consumption is limited. We therefore investigated whether consumption of a variety of vegetables and fruit is associated with gastric and esophageal cancer in the European Prospective Investigation into Cancer and Nutrition study. Data on food consumption and follow‐up on cancer incidence were available for 452,269 participants from 10 European countries. After a mean follow‐up of 8.4 years, 475 cases of gastric and esophageal adenocarcinomas (180 noncardia, 185 cardia, gastric esophageal junction and esophagus, 110 not specified) and 98 esophageal squamous cell carcinomas were observed. Diet Diversity Scores were used to quantify the variety in vegetable and fruit consumption. We used multivariable Cox proportional hazard models to calculate risk ratios. Independent from quantity of consumption, variety in the consumption of vegetables and fruit combined and of fruit consumption alone were statistically significantly inversely associated with the risk of esophageal squamous cell carcinoma (continuous hazard ratio per 2 products increment 0.88; 95% CI 0.79–0.97 and 0.76; 95% CI 0.62–0.94, respectively) with the latter particularly seen in ever smokers. Variety in vegetable and/or fruit consumption was not associated with risk of gastric and esophageal adenocarcinomas. Independent from quantity of consumption, more variety in vegetable and fruit consumption combined and in fruit consumption alone may decrease the risk of esophageal squamous cell carcinoma. However, residual confounding by lifestyle factors cannot be excluded.
CD99 is a novel prognostic stromal marker in non‐small cell lung cancer
Authors: Edlund Karolina , Lindskog Cecilia , Saito Akira , Berglund Anders , Pontén Fredrik , Göransson‐Kultima Hanna , Isaksson Anders , Jirström Karin , Planck Maria , Johansson Leif , Lambe Mats , Holmberg Lars , Nyberg Fredrik , Ekman Simon , Bergqvist Michael , Landelius Per , Lamberg Kristina , Botling Johan , Östman Arne , Micke Patrick 
Published: March 2012

AbstractThe complex interaction between cancer cells and the microenvironment plays an essential role in all stages of tumourigenesis. Despite the significance of this interplay, alterations in protein composition underlying tumour–stroma interactions are largely unknown. The aim of this study was to identify stromal proteins with clinical relevance in non‐small cell lung cancer (NSCLC). A list encompassing 203 stromal candidate genes was compiled based on gene expression array data and available literature. The protein expression of these genes in human NSCLC was screened using the Human Protein Atlas. Twelve proteins were selected that showed a differential stromal staining pattern (BGN, CD99, DCN, EMILIN1, FBN1, PDGFRB, PDLIM5, POSTN, SPARC, TAGLN, TNC and VCAN). The corresponding antibodies were applied on tissue microarrays, including 190 NSCLC samples, and stromal staining was correlated with clinical parameters. Higher stromal expression of CD99 was associated with better prognosis in the univariate (p = 0.037) and multivariate (p = 0.039) analysis. The association was independent from the proportion of tumour stroma, the fraction of inflammatory cells and clinical and pathological parameters like stage, performance status and tumour histology. The prognostic impact of stromal CD99 protein expression was confirmed in an independent cohort of 240 NSCLC patients (p = 0.008). Furthermore, double‐staining confocal fluorescence microscopy showed that CD99 was expressed in stromal lymphocytes as well as in cancer‐associated fibroblasts. Based on a comprehensive screening strategy the membrane protein CD99 was identified as a novel stromal factor with clinical relevance. The results support the concept that stromal properties have an important impact on tumour progression.
Regulation of the DLG tumor suppressor by β‐catenin
Authors: Subbaiah Vanitha Krishna , Narayan Nisha , Massimi Paola , Banks Lawrence 
Published: March 2012

AbstractThe discs‐large (DLG) tumor suppressor plays essential roles in regulating cell polarity and proliferation. It localizes at sites of cell–cell contact where it acts as a scaffold for multiple protein interactions, including with the adenomatous polyposis coli (APC) tumor suppressor, which in turn regulates β‐catenin. Furthermore, many tumor types including breast and colon have increased levels of β‐catenin activity with correspondingly low levels of DLG expression. Here we provide evidence of a direct functional link between these apparently separate phenomena. We show that overexpressed β‐catenin can enhance the turnover of DLG in a proteosome dependent manner. This effect is specific to DLG and is not seen with two other PDZ domain‐containing targets of β‐catenin, MAGI‐1 and Scribble. Furthermore, siRNA‐mediated ablation of endogenous β‐catenin expression also enhances DLG stability. β‐catenin‐induced degradation of DLG appears to be a consequence of a direct association between the two proteins and requires β‐catenin PDZ binding potential. In contrast, the enhanced turnover of DLG requires the unique N‐terminal sequences and its PDZ domains. Finally, we also show that the capacity of DLG to inhibit transformed cell growth in an oncogene cooperation assay is inhibited by β‐catenin. Taken together these studies suggest that one mechanism by which deregulated β‐catenin can contribute to tumorigenesis is through enhancing DLG degradation.
Indiscriminate behavior observed in the strange situation among institutionalized toddlers: Relations to caregiver report and to early family risk
Authors: Oliveira Paula S. , Soares Isabel , Martins Carla , Silva Joana R. , Marques Sofia , Baptista Joana , Lyons‐Ruth Karlen 
Published: March 2012

AbstractSocially disinhibited or indiscriminate behavior (IB) has traditionally been investigated using caregiver reports. More recently, an observational measure based on the Strange Situation Procedure (M. Ainsworth, M. Blehar, E. Waters, & S. Wall, 1978), the Rating of Infant and Stranger Engagement (RISE; C. Riley, A. Atlas‐Corbett, & K. Lyons‐Ruth, 2005), was validated in home‐reared at‐risk children. The present study aimed to validate the RISE in an institutionally reared sample using the caregiver report, to assess whether IB assessed with the RISE was elevated among the institutionalized children, and to explore potential risk factors associated with IB. The study was conducted among 74 institutionalized toddlers aged 11 to 30 months. Sociodemographic questionnaires were used to assess pre‐admission experiences, and aspects of institutional placement were coded from the children's files in the institution and staff's report. Institutionalized children displayed high frequencies of IB as assessed on the RISE, and this instrument was validated against caregiver report. Pre‐admission experiences of the institutionalized children in their biological families—namely, prenatal risk and maternal emotional neglect risk—predicted IB. Results suggest that the RISE is adequate to use among institutionally reared toddlers and point to aspects of the early familial environment that may be implicated in IB.
Regulatory disorders in early childhood: Correlates in child behavior, parent–child relationship, and parental mental health
Authors: Postert Christian , Averbeck‐Holocher Marlies , Achtergarde Sandra , Müller Jörg Michael , Furniss Tilman 
Published: March 2012

AbstractAccording to the guidelines published by the German Society of Child and Adolescent Psychiatry, Psychosomatics and Psychotherapy (2007), regulatory disorders of early childhood are characterized by a symptom triad, including (a) behavioral symptoms such as excessive crying, sleeping, or feeding problems; (b) a disturbed parent–child relationship; and (c) parental psychopathology. On the basis of a clinic–referred sample of 162 children, we examined whether children with and without regulatory symptoms differed in the quality of parent–child relationship and parental mental health, and how often the criteria of the symptom triad were fulfilled in the group of children with regulatory symptoms. In addition, emotional and behavior problems were compared in children with and without regulatory symptoms. Children with regulatory symptoms and children with other psychiatric symptoms did not differ with respect to child–parent relationship quality. However, parents of children with regulatory symptoms scored higher on the Symptom Checklist 90 Items‐Revised (G.H. Franke, 2002) than did the other parents. On the Child Behavior Checklist (T.M. Achenbach & L.A. Rescorla, 2000), children with regulatory symptoms tended to show more somatic problems, but they showed significantly less withdrawn behavior than did the other children. Of the 67 children with regulatory symptoms, only 11 (16.4%) fulfilled all three criteria of a regulatory disorder.
FORMULATION AND IMPLEMENTATION OF POLICIES TO DEAL WITH GROUNDWATER OVERUSE IN MOROCCO: WHICH SUPPORTING COALITIONS?
Authors: Faysse Nicolas , EL Amrani Mohamed , EL Aydi Soufiane , Lahlou Ahmed 
Published: March 2012

ABSTRACTThe Moroccan administration is showing increasing interest in designing policies to tackle groundwater overuse. In particular, it aims to set up aquifer management contracts for the main overused aquifers, which should bring together broad coalitions of actors around coordinated supply and demand policies. This article analyses to what extent the actors involved in groundwater use and management are coming together for the definition and implementation of policies to deal with groundwater overuse in four regions. A broad coalition has been built around the design of an aquifer management contract only in the Souss. Catchment management agencies and the Ministry of the Interior collaborate to control the drilling of boreholes in the Saiss, Souss and Berrchid regions. Large‐scale farmers in the Souss support the Ministry of Agriculture and the Souss Massa catchment management agency in developing more water resources. Small‐scale farmers were involved in none of these coalitions. The depth of the aquifer, the importance of the groundwater economy in each region, and the motivation and capacities of actors help explain the diversity in the coalitions that were created. While such elements help define the way coalition building may be supported in each case, in all cases particular human resources and skills will be needed to catalyse such coalitions, as will innovative ways to include small‐scale farmers. Copyright © 2012 John Wiley & Sons, Ltd.
Molecular and pharmacological evidence for a facilitatory functional role of pre‐synaptic GLUK2/3 kainate receptors on GABA release in rat trigeminal caudal nucleus
Authors: Samengo I. , Currò D. , Navarra P. , Barrese V. , Taglialatela M. , Martire M. 
Published: March 2012

AbstractBackgroundGamma‐aminobutyric acid (GABA) and glutamate (GLU) are involved in nociceptive signals processing in the trigeminal system. In this study, we investigated the influence of excitatory transmission on GABA release in nerve terminals isolated from the rat trigeminal caudal nucleus (TCN).MethodsWe utilize biochemical (superfused synaptosomes loaded with [3H]GABA) and morphological (immunofluorescence experiments with specific antibody) techniques.ResultsOur results show that GLU potentiates the release of [3H]GABA evoked by 9, 15 and 30 mM [K+]e; 15 mM [K+]e‐evoked [3H]GABA release was also reinforced by domoate and kainate (KA), two naturally occurring GLU‐receptor agonists. The enhancement of 15 mM [K+]e‐evoked [3H]GABA release produced by 100 μM KA was abolished by NBQX, a mixed AMPA/KA receptor antagonist, but was not affected by GYKI52466, a selective AMPA receptor antagonist. ATPA, a selective agonist for KA receptors containing the GLUK1 subunit, had no effect on depolarization‐induced [3H]GABA release, and UBP310, which selectively antagonizes these same receptors, failed to reverse the KA‐induced potentiation of 15 mM [K+]e‐evoked [3H]GABA release. The KA‐induced potentiation was also unaffected by concanavalin A (10 μM), a positive allosteric modulator of GLUK1‐ and GLUK2‐containing KA receptors.Immunofluorescence experiments revealed that GABAergic nerve terminals in the TCN differentially expressed GLUK subunits, with GLUK2/3‐positive terminals being twice more abundant than GLUK1‐containing synaptosomes.ConclusionsThese findings indicate that pre‐synaptic KA receptors facilitating GABA release from TCN nerve terminals mainly express GLUK2/GLUK3 subunits, supporting the notion that different types of KA receptors are involved in the various stages of pain transmission.
Engaging Preservice Teachers in Disciplinary Literacy Learning Through Writing
Authors: Pytash Kristine E. 
Published: March 2012

AbstractThe field of content area literacy instruction is shifting from a general understanding of literacy towards disciplinary literacy. Much of the work in the field of disciplinary literacy has focused on reading, while writing has often been overlooked. This article summarizes the findings of a qualitative case study of two preservice teachers as they experienced a unit of study during a Reading in the Content Areas course. Described in the article is how the unit of study as a writing assignment encompasses three aspects of disciplinary literacy: (1) discourses and practices in disciplinary learning and literacy, (2) identities and identifications in disciplinary learning and literacy, and (3) knowledge in disciplinary learning and literacy. Results found the unit of study enhanced preservice teachers’ knowledge of their discipline and writing instruction. The unit of study approach to writing instruction may offer literacy educators a way to engage preservice teachers in writing specific to their discipline.
NONPARAMETRIC ESTIMATION OF ENTRY COST IN FIRST‐PRICE PROCUREMENT AUCTIONS
Authors: Xu Pai 
Published: March 2012

SUMMARYIn this paper, I investigate Samuelson's low‐price auction model with entry costs. The model's equilibrium implies that the distribution of bids is truncated at the threshold for participation. I use the model to estimate the cost of participation in Michigan highway procurement auctions. The null hypothesis of zero entry costs is rejected. Using my empirical results, I then construct an estimate of the optimal auction, which employs regular policy tools such as entry fees. Finally, I demonstrate the savings that the Michigan government could have made on payments if optimal auctions had been employed. Copyright © 2012 John Wiley & Sons, Ltd.
Tamoxifen‐loaded thiolated alginate‐albumin nanoparticles as antitumoral drug delivery systems
Authors: Martínez A. , Benito‐Miguel M. , Iglesias I. , Teijón J. M. , Blanco M. D. 
Published: March 2012

AbstractNanoparticles based on disulfide bond reduced bovine serum albumin and thiolated alginate (alginate‐cysteine conjugate) have been prepared by coacervation method and have been loaded with tamoxifen (TMX). The TMX load into the nanoparticles was optimized (4–6 μg/mg NP) by freeze‐drying the systems before the loading procedure. Maximum TMX release (45–52%) took place between 2 and 25 h. Cytotoxicity of unloaded nanoparticles in MCF‐7 and HeLa cells was not observed, although a small decrease in viability took place at very high concentration. Cell uptake of nanoparticles occurred in both cell types and the presence of polysaccharide in the nanoparticle composition allowed a better interaction with cells. The administration of 10 μM TMX by TMX‐nanoparticles was effective in both cellular lines, and the effect of the drug‐loaded systems on MCF‐7 cell cycle showed the efficacy of the TMX‐loaded nanoparticles. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
Surface characteristics and primary bone marrow stromal cell response of a nanostructured strontium‐containing oxide layer produced on a microrough titanium surface
Authors: Park Jin‐Woo , Kim Youn‐Jeong , Jang Je‐Hee , Suh Jo‐Young 
Published: March 2012

AbstractStrontium (Sr) has been successfully used for the treatment of osteoporotic bone, increasing new bone formation while reducing bone resorption by stimulating proliferation and differentiation of osteoblastic cells and inhibiting osteoclast function. In this study, Sr‐incorporated Ti oxide layer was produced on clinically relevant osteoconductive implant surface, that is, a grit‐blasted microrough Ti surface, by a simple hydrothermal treatment with the expectation of utilizing the osteoblast response enhancement effect of Sr for the future applications as a more osteoconductive surface of the permanent load‐bearing endosseous implants, without altering the original microrough surface features of grit‐blasted Ti at the micron‐scale. This surface exhibits a hierarchical structure (i.e., a nanoscale surface architecture of the Sr‐incorporated Ti oxide layer (SrTiO3) imposed on micron‐scale rough Ti structure) and Sr ion release into physiological solution. In vitro experiments using primary mouse bone marrow stromal cells (BMSCs) revealed that the hydrothermally produced SrTiO3 coating promotes both the early and late cell response of BMSCs grown on a microrough Ti surface, with notably enhanced attachment, spreading, focal adhesion, alkaline phosphatase activity, and expression of critical integrins and osteoblastic phenotype genes. These results indicate that a hydrothermally produced SrTiO3 coating improves the osteoconductivity of the microrough Ti surface by enhancing both the early and late cell response of BMSCs. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
Reversal effects of pantoprazole on multidrug resistance in human gastric adenocarcinoma cells by down‐regulating the V‐ATPases/mTOR/HIF‐1α/P‐gp and MRP1 signaling pathway in vitro and in vivo
Authors: Chen Min , Huang Shu‐Ling , Zhang Xiao‐Qi , Zhang Bin , Zhu Hao , Yang Vincent W. , Zou Xiao‐Ping 
Published: March 2012

AbstractTo investigate reversal effects of pantoprazole (PPZ) on multidrug resistance (MDR) in human gastric adenocarcinoma cells in vivo and in vitro. Human gastric adenocarcinoma cell SGC7901 was cultured in RPMI‐1640 medium supplemented with 10% fetal bovine serum and antibiotics in a humidified 5% CO2 atmosphere at 37°C. Adriamycin (ADR)‐resistant cells were cultured with addition of 0.8 µg/ml of ADR maintaining MDR phenotype. ADR was used to calculate ADR releasing index; CCK‐8 Assay was performed to evaluate the cytotoxicity of anti‐tumor drugs; BCECF‐AM pH‐sensitive fluorescent probe was used to measure intracellular pH (pHi) value of cells, whereas pH value of medium was considered as extracellular pH (pHe) value; Western blotting and immunofluorescent staining analyses were employed to determine protein expressions and intracellular distributions of vacuolar H+‐ATPases (V‐ATPases), mTOR, HIF‐1α, P‐glycoprotein (P‐gp), and multidrug resistant protein 1 (MRP1); SGC7901 and SGC7901/ADR cells were inoculated in athymic nude mice. Thereafter, effects of ADR with or without PPZ pretreatment were compared by determining the tumor size and weight, apoptotic cells in tumor tissues were detected by TUNEL assay. At concentrations greater than 20 µg/ml, PPZ pretreatment reduced ADR releasing index and significantly enhanced intracellular ADR concentration of SGC7901 (P < 0.01). Similarly, PPZ pretreatment significantly decreased ADR releasing index of SGC7901/ADR dose‐dependently (P < 0.01). PPZ pretreatment also decreased cell viabilities of SGG7901 and SGC7901/ADR dose‐dependently. After 24‐h PPZ pretreatment, administration of chemotherapeutic agents demonstrated maximal cytotoxic effects on SGC7901 and SGC7901/ADR cells (P < 0.05). The resistance index in PPZ pretreatment group was significantly lower than that in non‐PPZ pretreatment group (3.71 vs. 14.80). PPZ at concentration >10 µg/ml significantly decreased pHi in SGC7901 and SGC7901/ADR cells and diminished or reversed transmembrane pH gradient (P < 0.05). PPZ pretreatment also significantly inhibited protein expressions of V‐ATPases, mTOR, HIF‐1α, P‐gp, and MRP1, and alter intracellular expressions in parent and ADR‐resistant cells (P < 0.05). In vivo experiments further confirmed that PPZ pretreatment could enhance anti‐tumor effects of ADR on xenografted tumor of nude mice and also improve the apoptotic index in xenografted tumor tissues. PPZ pretreatment enhances the cytotoxic effects of anti‐tumor drugs on SGC7901 and reverse MDR of SGC7901/ADR by downregulating the V‐ATPases/mTOR/HIF‐1α/P‐gp and MRP1 signaling pathway. J. Cell. Biochem. 113: 2474–2487, 2012. © 2012 Wiley Periodicals, Inc.