bluuebunny/crossref_metadata_2025_split huggingface dataset:
Myogenic dedifferentiation involves a p53-dependent reorganization of PLK4 localization during centrosome regeneration
Authors: Subramanian Elaiyaraja , Brito Gonçalo , Kumar Anoop , Kirkham Matthew , Simon András 
Published: June 2024

AbstractMultinucleated skeletal muscle cells are stably withdrawn from the cell cycle in most vertebrates. Muscle dedifferentiation is however naturally occurring during limb regeneration in newts and can be artificially induced in mammalian myotubes. Dedifferentiation involves fragmentation of myofibers and myotubes into mononucleate cells which subsequently reenter the cell cycle, and give rise to proliferative progeny. Here we addressed the dynamics of centrosomes, which are key organelles for cell proliferation during myogenic differentiation and dedifferentiation. We show that, in contrast to their mammalian counterparts, newt muscle cells retain centrosomes during differentiation and demonstrate that regeneration of centrosomes in dedifferentiated mouse muscle cells depends on inhibition of the tumor suppressor p53. We also find that regulation of the subcellular localization of Polo-Like Kinase 4 rather than its expression level is a hallmark of myogenic differentiation and dedifferentiation, identifying a novel cellular process underlying the plasticity of the differentiated state.
The spatial layout of antagonistic brain regions is explicable based on geometric principles
Authors: Leech Robert , Braga Rodrigo M , Haydock David , Vowles Nicholas , Jefferies Elizabeth , Bernhardt Boris , Turkheimer Federico , Alberti Francesco , Margulies Daniel , Sherwood Oliver , Jones Emily JH , Smallwood Jonathan , Váša František 
Published: June 2024

AbstractBrain activity emerges in a dynamic landscape of regional increases and decreases that span the cortex. Increases in activity during a cognitive task are often assumed to reflect the processing of task-relevant information, while reductions can be interpreted as suppression of irrelevant activity to facilitate task goals. Here, we explore the relationship between task-induced increases and decreases in activity from a geometric perspective. Using a technique known as kriging, developed in earth sciences, we examined whether the spatial organisation of brain regions showing positive activity could be predicted based on the spatial layout of regions showing activity decreases (and vice versa). Consistent with this hypothesis we established the spatial distribution of regions showing reductions in activity could predict (i) regions showing task-relevant increases in activity in both groups of humans and single individuals; (ii) patterns of neural activity captured by calcium imaging in mice; and, (iii) showed a high degree of generalisability across task contexts. Our analysis, therefore, establishes that antagonistic relationships between brain regions are topographically determined, a spatial analog for the well documented anti-correlation between brain systems over time.Significance StatementIt is well documented that brain activity changes in response to the demands of different situations, although what gives rise to the observed cortical activity patterns remains poorly understood. Using analytic tools from earth sciences, we examined whether the landscape of regional changes in activity emerge from a set of common topographical causes. Using only regions showing decreases in activity, we could predict the landscape of regions showing increases in activity using fMRI in humans and calcium imaging in mice. Our results suggest topographical principles determine the landscape of peaks and valleys in brain activity -- a spatial analog for the well documented anti-correlation between sets of brain regions over time.
Lack of an atypical PDR transporter generates an immunogenicCryptococcus neoformansstrain that drives a dysregulated and lethal immune response in murine lungs
Authors: Winski Christopher J. , Stuckey Peter V. , Marrufo Armando M. , Ross Robbi L. , Agyei Georgina , Chapman Sarah , Santiago-Tirado Felipe H. 
Published: June 2024

ABSTRACTCryptococcus neoformansis an opportunistic fungal pathogen responsible for >150,000 deaths every year with a mortality rate as high as 81%. This high medical burden is due, in part, to an incomplete understanding of its pathogenesis. In a previous study, we identified a cryptococcal atypical pleiotropic drug resistance (PDR) transporter,PDR6, that regulated antifungal resistance and host interactions. Here, we follow-up on the role ofPDR6in cryptococcal virulence.In vivo, mice infected with thepdr6Δ strain display altered symptomatology and disease progression. Specifically, we observed a significant increase in the innate immune cell populations in thepdr6Δ-infected mice when compared to their WT-infected littermates. Furthermore, quantification of pulmonary cytokines/chemokines revealed a robust increase of pro-inflammatory cytokines in mice infected with thepdr6Δ mutant strain. Whereas antifungal treatment ofpdr6Δ-infected animals did not affect survival, treatment with a corticosteroid significantly extended survival, highlighting the importance of a balanced/controlled host immune response. We determined that the hyper-inflammatory immune response occurs, in part, because the loss of the Pdr6 transporter indirectly alters the cryptococcal cell wall architecture and results in the increased exposure of chitin, β-glucan, and other cryptococcal-specific pathogen associated molecular patterns. Taken together, this study provides clinical insights regarding cryptococcal pathogenesis while also providing additional functions of PDR-type ATP-binding cassette (ABC) transporters in pathogenic fungi.IMPORTANCEYeasts of theCryptococcusgenus, especiallyC. neoformans, can cause disease with unacceptably high mortality. This is due to delays in diagnostics, ineffective treatments, and an incomplete understanding of the interactions between this fungus and our immune system. In this study, we expand our knowledge of the biological function of thePDR6gene, particularly its effect on modulating the host’s immune response. Normally,C. neoformans’s infections are characterized by an anti-inflammatory response that is unable to control the yeast. In the absence ofPDR6, the response to the infection is a dysregulated pro-inflammatory response that initially controls the fungi but eventually results in death of the host due to too much tissue damage. This is due, in part, to an altered fungal surface. Given the dual role ofPDR6in modulating antifungal sensitivity and immune responses, this work provides important insights that may lead to new or improved therapeutics.
In-vitroresensitization of multidrug resistant clinical isolates ofEnterococcus faeciumandfaecalisthrough phage-antibiotic synergy
Authors: Ghatbale Pooja , Sah Govind Prasad , Dunham Sage , Khong Ethan , Blanc Alisha , Monsibais Alisha , Garcia Andrew , Schooley Robert T. , Cobián Güemes Ana G. , Whiteson Katrine , Pride David T. 
Published: June 2024

AbstractBacteriophages are an increasingly attractive option for the treatment of antibiotic resistant infections, but their efficacy is difficult to discern due to confounding effects of antibiotics. Phages are generally delivered in conjunction with antibiotics, and thus, when patients improve it’s unclear whether the phages, antibiotics or both are responsible. This question is particularly relevant for enterococcus infections, as limited data suggest phages might restore antibiotic efficacy against resistant strains. Enterococci can develop high-level resistance to vancomycin, a primary treatment. We assessed clinical and laboratory isolates ofEnterococcus faeciumandEnterococcus faecalisto determine whether we could observe synergistic interactions between phages and antibiotics. We identified synergy between multiple phages and antibiotics including linezolid, ampicillin, and vancomycin. Notably, antibiotic susceptibility did not predict synergistic interactions with phages. Vancomycin resistant isolates (n=6) were eradicated by the vancomycin-phage combination as effectively as vancomycin susceptible isolates (n=2). Transcriptome analysis revealed significant gene expression changes under antibiotic-phage conditions, especially for linezolid and vancomycin, with upregulated genes involved in nucleotide and protein biosynthesis and downregulated stress response and prophage-related genes. While our results do not conclusively determine the etiology of the observed synergistic interactions between antibiotics and phages, they do confirm and build upon previous research that observed these synergistic interactions. Our work highlights how using phages can restore the effectiveness of vancomycin against resistant isolates. This finding provides a promising, although unexpected, strategy for moving forward with phage treatments for Vancomycin Resistant Enterococcus infections.
Cluster social capital’s influence on regional public brands in specialty agriculture: a value co-creation perspective
Authors: Liu Yizhi , Fu Yi , Liang Zihan , Liu Yu 
Published: June 2024

PurposeThis study aims to explore the collaborative value creation process in the context of regional public brands within the specialty agricultural products domain. It examines the role of cluster social capital in facilitating stakeholder interactions and cooperation, thereby enhancing the value of these brands.Design/methodology/approachThe study adopts a value co-creation theoretical framework to construct a relational model that links cluster social capital, brand interaction, stakeholder perceived value, and the value of regional public brands. An empirical analysis is conducted using a sample of 404 valid responses to test this model and assess the influence of cluster social capital on brand value co-creation.FindingsThe findings reveal that cluster social capital significantly impacts stakeholder participation in value co-creation activities, thereby playing a crucial role in enhancing the value of regional public brands. The study also finds that brand interactions significantly affect stakeholder perceived value and the overall brand value, with perceived value acting as a partial mediator in these relationships.Originality/valueThis research contributes to the understanding of value co-creation in regional public brands, particularly in the agricultural sector. It provides new insights into how cluster social capital influences the value creation process, offering valuable implications for policymakers and practitioners working to develop and promote regional public brands in the specialty agricultural products market.
The nexus between FDI and human development: non-linear evidence from South Asia
Authors: Dash Ranjan , Gupta Deepa , Mishra Aditi 
Published: June 2024

PurposeHuman development is critical for fostering economic growth and development. Given the importance of human development, this study examines the asymmetric impact of Foreign Direct Investment (FDI) on human development by decomposing total FDI into positive and negative shocks in five South Asian countries from 1990 to 2021.Design/methodology/approachThe study uses the panel Non-linear Autoregressive Distributive Lag model (NARDL) to examine asymmetric long and short-run effects of FDI. Further, the direction of causality between HDI and FDI is examined using the recently developed (Joudis et al., 2021) panel granger non-causality test.FindingsThe positive and negative FDI shocks positively impact HDI, but positive shocks have a higher effect than negative shocks in the long run. The Wald Test rejects the long-run symmetric effect, confirming the asymmetric relationship between FDI and human development. More importantly, causality results reveal the FDI-led HDI and HDI-led FDI development in South Asia.Practical implicationsFDI should be encouraged by formulating a well-tailored policy intervention. The development policies should be interlinked with FDI policies. Absorptive capacities such as infrastructure facilities, a threshold level of human capital, and institutions should be strengthened to attract higher FDI into high-tech sectors.Originality/valueUnlike the previous empirical studies, this study provides asymmetric evidence between FDI and human development in South Asia.Peer reviewThe peer review history for this article is available at: https://publons.com/publon/10.1108/IJSE-05-2023-0380.
Examining the interplay between managerial ties, dynamic capabilities and innovation climate in driving balanced and combined ambidextrous innovation
Authors: Alhammadi Bader , Khalid Khalizani , Ahmad Syed Zamberi , Davidson Ross 
Published: June 2024

Purpose
This paper aims to adopt the dynamic capabilities view to investigate the relationship between managerial ties (i.e. business and political ties), dynamic capabilities and innovation climate on ambidextrous innovation (i.e. balanced and combined ambidextrous innovation), in the renewable and sustainable energy context. It also examines the mediating effects of dynamic capabilities between managerial ties and ambidextrous innovation (i.e. balanced and combined ambidextrous innovation), and moderating effects between dynamic capabilities and ambidextrous innovation relationships.

Design/methodology/approach
Multilevel analyses conducted using AMOS 26 on 288 employees working in 47 UAE energy firms.

Findings
Results found that business ties influences balanced and combined ambidextrous innovation indirectly, whereas political ties only impact combined ambidextrous innovation indirectly through dynamic capabilities. Dynamic capabilities insignificantly mediated managerial ties–ambidextrous innovation and political ties–balanced ambidextrous innovation relationships, with stronger indirect effect on combined than on the balanced dimension. Findings also indicate that innovation climate is the crucial moderator between dynamic compatibilities and ambidextrous innovation, as well as balanced and combined ambidextrous innovation, with stronger effect on balanced dimension than the combined.

Originality/value
This study addresses recent calls by highlighting the role of dynamic capabilities, an important yet underexplored organizational capabilities in the innovation and ambidexterity literature. Also, this study advances insight into how balanced and combined exploration–exploitation innovation and dynamic capabilities are connected and enhances the understanding into how organizational factors stimulate dynamic capabilities leading to superior innovation.
“Having just the right answer is almost as worthless as not having an answer’’: conceptualizing the information needs of undergraduate engineers
Authors: Dodson Samuel 
Published: June 2024

PurposeThis paper explores the information needs and behaviors of undergraduate engineers.Design/methodology/approachThe paper reports on a qualitative study employing semi-structured interviews with 18 students.FindingsThe study identified the types of information needs undergraduate engineers encounter while working on problem solving tasks and the strategies they use to resolve these needs. The findings reveal that students often encounter difficulties due to a lack of procedural knowledge rather than conceptual gaps or misunderstandings. Students look for step-by-step solutions to address their information needs and become more efficient problem-solvers. However, most instructors do not provide answers or solutions, leaving students uncertain about their progress and unable to correct their mistakes. Consequently, students seek information from their peers, including step-by-step solutions and access to previous course materials. They use file-sharing and instant messaging platforms like Google Drive and Facebook Messenger as covert means of seeking help, sharing solutions and engaging in coursework-related discussions.Originality/valueThe findings enrich the theory of information needs by delineating between conceptual and procedural information needs. These findings also underscore the significant role that classmates and friends play as sources of information. The study offers implications for conceptual development of information needs, and for instructors to provide solutions and support sharing between peers on official platforms.
Exploring the motivations behind artificial intelligence adoption for building resilient supply chains: a systematic literature review and future research agenda
Authors: Vishwakarma Laxmi Pandit , Singh Rajesh Kr , Mishra Ruchi , Venkatesh Mani 
Published: June 2024

PurposeThe study aims to synthesize existing knowledge and proposes a research framework for building a resilient supply chain (SC) through artificial intelligence (AI) technology. It also identifies existing literature gaps and paves the way for a future research agenda.Design/methodology/approachA systematic literature review has been carried out to identify the peer-reviewed articles from Scopus and Web of Science databases. Then, the selected articles published between 2012 and 2023 are analyzed using descriptive and thematic analysis methods to unearth research gaps and offer new research directions.FindingsDescriptive and thematic analysis reveals the overall development of literature on the role of AI for supply chain resilience (SCR). Based on the findings of the thematic analysis, the motivation, application, capability and outcome (MACO) framework has been developed and propositions have been proposed. Several future research directions have also been suggested in terms of theory, context and methodology (TCM).Practical implicationsThe study provides a fresh perspective on the integration of AI technology within the realm of SCR. The developed MACO framework serves as a practical tool for supply chain management (SCM) professionals, offering a nuanced understanding of AI's applications across various functional areas to streamline operations, minimize waste and optimize resource utilization, thereby helping them in strategic planning.Originality/valueThis study contributes to the literature on the role of AI for building SCR by uncovering gaps, offering research directions and developing propositions for future research directions.
Exploring the uncharted influence of family social capital in entrepreneurial ecosystems
Authors: Appleton Samuel Wayne , Pavlou Christodoulos 
Published: June 2024

PurposeThe purpose of this research is to develop theory, thereby attending to the existing knowledge gap regarding the impact of family firms on entrepreneurial ecosystems (EEs). Reducing such a gap is both timely and relevant given the ubiquity of family firms across the globe and the lack of theoretical development at the intersection of EE and family firm literatures. By employing social capital theory in a propositional theorizing approach, this article presents unique propositions that enrich current understanding of the EE phenomenon.Design/methodology/approachOur method adopts a three-step propositional theorizing approach. The first step outlines our conceptualization, drawing on social capital theory and identifying multiple levels of analysis pertaining to EEs and family firms. The second step precisely identifies the constructs used for the theorization process, drawing upon relevant literature. The third step involves proposition building, which produces our findings.FindingsAs a result of our propositional theorizing method, we developed 10 theoretical propositions to explain interactions between family members, nonfamily entrepreneurs, family firms and new ventures in the EE, thereby focusing on the social elements of the EE and reducing its conceptual complexity while extending the explanatory power of family social capital in the EE.Research limitations/implicationsDespite being increasingly relevant in research, policy and practice discourse, EEs remain under theorized. By theorizing in this context, we provide explanations of the mechanisms to explain social interactions between family members, nonfamily entrepreneurs, family firms and new ventures and how such interactions are likely to provide better access to the untapped resources in the EE. Furthermore, our theorization also identifies underexplored research areas paving the way for future scholars.Practical implicationsThis article is relevant to practitioners and policymakers interested in creating balanced, inclusive and effective EE policies and interventions. Our theorization generates insights that complement a bottom-up approach where the state assumes a facilitating role for actors such as family firms to positively impact their EE. This research is both timely and necessary because, if unaddressed, it will lead to ineffective and potentially exclusionary policies and EE interventions.Originality/valueWe contribute to the literature by synthesizing the two domains and thereby advancing knowledge at the intersection of EE and family firm literatures. We strengthen the link between two burgeoning research areas through a propositional theorizing mode of theory development. Under the assumptions of a grand theory, social capital theory, we highlight the benefits that derive from social interactions in the EE between family firms and other EE actors.
Making Knowledge Claims from Qualitative Interviews: A Typology of Epistemological Modes
Authors: Whittle Andrea , Reissner Stefanie 
Published: June 2024

AbstractQualitative interviewing is the most common qualitative research method in management studies. However, researchers using this method tend to use a distinct ‘packages’ of practices, each of which is underpinned by a distinct onto‐epistemological paradigm. In this paper, we contribute to the understanding of how paradigms influence research by examining how researchers make an ‘epistemological leap’ from their interview data to a claim to know something about a phenomenon outside of the interview situation. Using illustrative examples from published management research, we develop a typology of five epistemological modes that differ according to how far researchers ‘leap’ and what they ‘leap’ to when making knowledge claims from interview data. We conclude by outlining the implications of our typology for those involved in conducting, teaching and evaluating qualitative interview research.
Clinical validation of the nursing outcome “Swallowing status: pharyngeal phase” in patients with laryngeal cancer
Authors: Duan Ruirui , Ding Yongxia , Tian Yanzhi , Yang Huixian , Xu Jing 
Published: June 2024

AbstractAims and objectivesThis study aimed to clinically validate the nursing outcome “Swallowing status: pharyngeal phase” (1013).MethodsA two‐stage study was designed: (1) Chinese translation and cultural adaptation and (2) clinical validation. Internal consistency and interrater reliability tests were performed on 285 patients with laryngeal cancer, and an additional 130 patients were randomly selected from the 285 patients as an independent sample. Criterion‐related validity tests were performed using the standardized swallowing assessment (SSA). Nursing outcome sensitivity was detected by scoring two time points.ResultsThe Cronbach's alpha coefficients were 0.951 for the nursing outcome and 0.942–0.965 for each indicator. The interclass correlation coefficient (ICC) values for each indicator ranged from 0.73 to 0.929. The scores of the nursing outcome were negatively correlated with the SSA scores (r = −0.555, p < 0.01). With the exception of two indicators, there was a significant difference (p < 0.05) between the total scores of the scale and its 11 indicator scores for the two time points. The results indicated that the nursing outcome “Swallowing status: pharyngeal phase” (1013) exhibited satisfactory psychometric properties and high sensitivity to change.ConclusionsThe nursing outcome “Swallowing status: pharyngeal phase” (1013) demonstrated good reliability, validity, and sensitivity in patients with laryngeal cancer.Implications for nursing practiceThe nursing outcome “Swallowing status: pharyngeal phase” (1013) can be used to assess swallowing function in patients with laryngeal cancer and provide guidance for the development of rehabilitation intervention plans and nursing care.