bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of platinum-resistance related small GTPase binding signatures to predict the prognosis of ovarian cancer by machine learning and integrated bioinformatic analyses
Authors: Zhong Ya-jun , Zhu Yi-lin , Wang Shi-qi , Wang Yuan-rong , Bu Lan-ying , Zhao Rui-heng , Zhou Ying , Kong Wei-yu , Zhou Hong 
Published: May 2024

Abstract

Background
High grade serous ovarian cancer (HGSOC) had high lethality due to its high relapse rate and acquired drug resistance. The tumor immune microenvironment (TIME) of HGSOC was heterogeneous, and mostly immune cold. We aimed to build the bridge between platinum-resistance related signatures and patient overall survival (OS).
Methods
The RNA sequencing data from GSE160626 was used for extraction of platinum-resistance related genes. The TCGA-OV cohort were fitted into 101 kinds of machine learning methods, and the validation cohort included GSE9899, GSE63885 and GSE26193. Numerous methods including the Cindex, receiver operating characteristic curve (ROC), univariate and multivariate Cox regression, and the decision curve analysis (DCA) were applied to detect the performances of platinum-resistance related risk score (PRRS) and a PRRS based nomogram. The single-cell RNA sequencing data and Spatial Transcriptomics data were used to determine the risky cell types correlated with our PRRS.
Results
Based on platinum-resistance related genes, we conducted consensus clustering and defined a platinum-resistance resembling cluster, which had significantly shorter OS. And with DEGs related to small GTPase between two clusters, we established a PRRS and a PRRS based nomogram, which had excellent performances in predicting OS of serous ovarian cancer patients. We further determined SPP1 + M2-like Macrophages were risky factors correlated with the PRRS, and determined ABCA1 and NDRG1 as the hub genes related to patient OS.
Conclusion
Small GTPase was a dominant feature of platinum-resistance resembling clusters. PRRS had terrific predicting value and correlated with SPP1 + M2-like Macrophages.
Minocycline alleviates microglial ferroptosis by inhibiting HO-1 during cerebral ischaemia reperfusion injury
Authors: Wang Lin , Wang Yao , Wu Mengyue , Jin Xing , Chen Yifei , Guo Zhenhuan , Meng Xiaowen , Zhang Jianyou , Ji Fuhai 
Published: May 2024

Abstract

Objective
Ischaemic stroke is a leading cause of death and disability in individuals worldwide. Cerebral ischaemia reperfusion injury (CIRI) usually leads to severe secondary injury and complications following reperfusion therapy. Microglia play critical roles in the inflammatory reaction of CIRI. However, less attention has been given to microglial death in this process. Our study aimed to explore microglial death in CIRI and the effect and mechanism of minocycline treatment on microglia
Methods
A middle cerebral artery occlusion (MCAO) model was applied to induce CIRI in rats. At 0 h, 24 h and 48 h postoperation, 45 mg/kg minocycline was intraperitoneally injected into the rats. Neurological deficit scoring, 2,3,5-triphenyltetrazolium chloride (TTC) staining, activated microglia and mitochondrial structure were observed and checked at 72 h after reperfusion. Moreover, an in vitro model of oxygen-glucose deprivation/reperfusion (OGD/R) model was established. BV-2 cells were treated with either various pharmacological inhibitors of cell death or minocycline. Cell viability, lipid peroxidation, mitochondrial structure and functioning, and labile Fe2+ and ferroptosis-associated gene/proteins levels were measured. Hemin was used for further validation after transcriptome analysis.
Results
In the MCAO and OGD/R models, ferroptosis was identified as a major form of microglial death. Minocycline inhibited microglial ferroptosis by reducing HO-1 expression. In addition, minocycline improved mitochondrial membrane potential, mitochondrial structures and microglial survival in vivo. Minocycline also decreased labile Fe2+ levels, lipid peroxidation, and ferritin heavy chain (FTH) expression and improved mitochondrial structure and functioning in vitro. HO-1 overexpression counteracted the protective effect of minocycline.
Conclusion
Ferroptosis is a major form of microglial death in CIRI. The mechanism of the protective role of minocycline in CIRI is partly dependent on its ability to effectively ameliorate microglial ferroptosis by reducing HO-1 expression. Therefore, targeting microglial ferroptosis is a promising treatment for CIRI.
Study on the anchorage mechanism and roof stability control technology of bolts in fractured or weak surrounding rock
Authors: Wang Jiyu , Zheng Xigui , Guo Xiaowei , Xin Wei , Wang Yonghui , Li Tao , Liu Longhe 
Published: May 2024

Abstract

The effect of bolt support in mining roadways with fractured or weak surrounding rock is poor, and the roof easily loses stability. Studying the support control technology of bolts on fractured surrounding rock is necessary. Based on the theories of pressure arches and combined arch support, the bolt anchorage and anchorage effect angle are proposed a model is established, and the optimal bolt anchorage is calculated. The influence of related factors on the bolt anchorage and the scope of bolt action are analysed via numerical simulation and experimental methods. The pretightening force of the bolts and the spacing between the rows of bolts are positively correlated and negatively correlated with the bolt anchorage, respectively. The compression zone conforms to the reinforced arch state when the bolt end is anchored. The experimental work shows that the maximum spacing a of the anchor rods and the length L of the anchor rods in the surrounding rock satisfy 4.28a < 2L < 5a. The support of fractured surrounding rock is discussed, and the concept of roof fall prevention and control with an increasing or constant pretightening force, high surface strength and reasonable support density as the core principles is proposed. A field test of the Youzhong Coal Mine shows that the corresponding support effect is good and that this work provides a new method for roof support of fractured surrounding rock.
LncRNA LINC00160 facilitates tumor progression via miR-629-3p/RAB13 axis in glioma
Authors: Jun Wu , Tian Da , Zhang Xuelei , Du Qinghua 
Published: May 2024

Abstract

Background: Glioma is a kind of malignant brain tumor with high mortality. LncRNA is involved in the regulation of glioma development. The relationship between lncRNA and glioma progression needs further study. Whether LINC00160 is involved in the progression of glioma remains unclear. The purpose of this study was to investigate the role and mechanism of LINC00160 in glioma.
Methods: Tumor tissues were collected and human cell lines were purchased. RNA was extracted using Trizol reagent and analyzed through qRT-PCR. The survival rate was examined according to LINC00160 expression. siRNAs were used to knock down LINC00160 in tumor cells. miRDB and TargetScan were used to predict RNA interactions. RNA interaction was verified by luciferase reporter assay. Cell growth was evaluated using CCK8, colony formation, and transwell assay respectively. TCGA data was used to analyze RAB13 expression.
Results: The expression of LINC00160 was increased in glioma. High expression of LINC00160 was associated with poor prognosis. LINC00160 knockdown suppressed tumor proliferation, migration and invasion. LINC00160 sponges miR-629-3p to inhibit its binding to RAB13 mRNA, resulting in RAB13 upregulation. RAB13 overexpression reversed the inhibitory effects of LINC00160 knockdown on proliferation, migration and invasion.
Conclusion:Our findings support that LINC00160 exerts oncogenic roles in glioma progression through miR-629-3p/RAB13 axis.
Sorting of mesoporous silica derivatives by random optical fields
Authors: Sadri Mohammad Hadi , Jamali Ramin , Khan Asif Jamal , Rehman Fozia , Moradi Ali-Reza 
Published: May 2024

Abstract

Mesoporous silica particles are promising candidates for drug delivery applications. In this paper, we first synthesize meso-porous silica MCM-41 and its derivative MCM-41GA with anchored glutaraldehyde bridges, and characterize them using a variety of techniques, including nitrogen adsorption/desorption, X-ray diffraction, NMR spectroscopy, scanning electron microscopy, and thermogravimetric analysis. Then, we employ random optical fields to sort mesoporous silica particles. Random optical fields by containing local intensity gradients throughout a wide range of field of view provide an elegant, easy-to-implement, and low-cost variant of multiple optical tweezers, which is known as speckle tweezers (ST). ST, similar to multiple optical tweezers, for manipulation tasks, such as trapping, sorting, and guiding of collection of micro and sub-micro objects in several disciplines including statistical physics, chemistry, microfluidics and material science. We show that ST can restrict, sieve, and sort MCM-41 and MCM-41GA particles. The different interaction of mesoporous silica variations with the applied ST may be attributed to the pre-applied modification and the differences in the porosity structure and distribution. Therefore, the results provide insight into the textural and chemical characteristics of mesoporous materials, contributing to a deeper understanding of their potential applications.
TREM2 inhibits rTp47-induced inflammasome activation and pyroptosis via targeting NLRP3
Authors: Weng Wenjia , Chang Linlin , Li Zhen , Bai Ruojing , Li Juan , Zhang Ming , Huang Xiaojie , Gao Yanqing 
Published: May 2024

Abstract

Background: Microglia surface receptors, particularly TREM2 (triggering receptor expressed on myeloid cells 2), play a crucial role as sensors for pathogen entry and can trigger robust immune responses. However, the specific role of TREM2 in regulating microglia's response to Treponema pallidum (T. pallidum) and its underlying functional mechanisms remain unclear. We aim to investigate the role of TREM2 in the inflammation caused by recombinant T. pallidum membrane protein Tp47 infection in microglia and to elucidate the associated mechanisms.
Methods: The effect of rTp47 on TREM2 expression in HMO6 cells and its mechanism were evaluated using Flow cytometry, Western Blot, and Quantitative Real-Time PCR. The effect of TREM2 on the pyroptosis of HMO6 cells was evaluated by ELISA using generated CRISPR/Cas9-Mediated Knockout (KO) Cell Line. The interaction between TREM2 and NLRP3 on HMO6 pyroptosis was assessed by co-immunoprecipitation. The levels of soluble TREM2 (sTREM2), inflammatory cytokines, and pyroptosis were measured in the cerebrospinal fluid of 19 neurosyphilis and 13 non-neurosyphilis patients by ELISA.
Results: rTp47 promoted the expression of TREM2. The pyroptosis rate of HMO6 cells did not change with increasing rTp47 concentration, but TREM2 knockout (KO) augmented rTp47-induced NLRP3/caspase-1 inflammasome activation and increased the expression of pro-inflammatory cytokine IL-1β. Furthermore, we found TREM2 inhibits HMO6 cells pyroptosis by interacting with NLRP3. In addition, increased levels of CSF sTREM2 and IL-10 were observed in neurosyphilis patients compared to non-neurosyphilis patients, while TGF-β levels were significantly lower. Conclusion: rTP47 can inhibit the activation of NLRP3 inflammasome by up-regulating the expression of TREM2, so as to prevent the pyroptosis of HMO6 cells.
Design and Development of a Three-Winding Coupled- Inductor DC-DC Converter Topology with SwitchStress
Authors: S Sanjay , G Ashok kumar. , gopal Renu , B Sowmidharan 
Published: May 2024

Abstract

This proposed paper presents a DC-DC boost converter topology for low input and high output voltage applications, such as photovoltaic systems, fuel cell systems, high-intensity discharge lamp (HID), and electric vehicles. The suggested configuration consists of a three-winding coupled-inductor, a single switch and two hybrid voltage multiplier cells. Furthermore, two independent hybrid voltage multiplier cells are in parallel when the single switch S is turned on, and they are in series when the switch S is turned off. So the advantages of the proposed converter structure are summarized as follows: a) A coupled inductor with three windings is introduced in the presented converter structure.The two secondary windings of the coupled inductor are respectively used to form a hybrid multiplier cell on the one hand, on the other hand, it increases the control freedom of the voltage gain, enhances the utility rate of magnetic core and power density, and reduces the stress of power components to provide a stable constant dc output voltage. b) The two hybrid multiplier cells can absorb synchronously the energy of stray inductance, which not only reduces the current stress of corresponding diodes, but also greatly alleviates the spike voltage of the main switch, which improves the efficiency. c) The two hybrid multiplier cells are connected in series to supply power energy for the load, so the voltage gain is extended greatly due to this particular structure.
Presence of Cutaneous Signs of Insulin Resistance with Central Obesity (CO-CSIR) in Asian Indians is a sensitive physical sign of Metabolic Syndrome (MetS)
Authors: Gora Anamika , Tiwari Pradeep , Saxena Aditya , Mandia Rajendra , Gupta Shalu , Lamoria Ravinder Kumar , Mathur Sandeep Kumar 
Published: May 2024

Abstract

Aim and objectives: To investigate cutaneous sign of insulin resistance, acanthosis nigricans (AN) and acrochordon (AC) in individual of central obesity (CO-CSIR) as a physical sign for prediction of metabolic syndrome (MetS) and the underlying adipose tissue pathology and the consequent pathophysiological trait in Asian Indians.
Methods: Design: Single center cross sectional study. Study subjects: 371 (aged 51.7±12.4; M: F ratio 210:161). Following parameters were investigated: Physical signs: cutaneous signs of insulin resistance, BMI, WC, HC, WHR, blood pressure. Biochemical parameters: FBG, lipid profile, HbA1c, HOMA-β. HOMA-IR. Radiological parameters: Abdominal visceral, subcutaneous and ectopic liver fat by MRI. Molecular Parameters: Genome wide transcription profile of adipose tissue biopsies in 85 individuals undergoing surgery for other indications.
Results: AN, AC and both were present respectively 50(13.3%), 27(7.2%) and 75(20.2%) individuals and they absent 216 individuals. Presence of AN and AC were associated with significantly higher BMI (6.4 X10-5), W:H ratio (0.04), WC (9.5 X 10-7), HOMA-IR (0.0002), glucose (1.11 x 10-10) and prevalence of T2D (100%) and MetS (83%). AC as compared to AN was associated with more ectopic fat and higher IR.CO-CSIR was found to be the best physical sign of MetS (94.8% sensitivity,57.5 % specificity, 86.4 precision with 95.1F1 score). MetS negative CO-CSIR individuals show high IR, ectopic fat deposition, hyperglycemia and prevalence of T2D.
Conclusion: CO-CSIR a promising physical sign of MetS and the underlying adipose tissue driven dysmetabolism in Asian Indians.
Capacity Assessment of the National Medicines Regulatory Authority in a Low -income Country
Authors: Thomas Fawzi , Abiri Onome T. , Conteh Thomas A. , Awodele Olufunsho 
Published: May 2024

Abstract

Background: Access to medical products of the required efficacy, quality and safety is essential for everyone’s health and wellbeing. To achieve this milestone, every country needs a robust and strong performing National Regulatory Authority (NRA) that is independent and outcome oriented. With the help of the World Health Organization (WHO), the global benchmarking tool is the gold standard used to assess the regulatory capacity of NRAs.
Objectives: This study assessed the capacity of the National Medicines Regulatory Authority in Sierra Leone to perform its regulatory functions.
Methods: This descriptive cross-sectional study used both qualitative and quantitative approaches. A self-administered questionnaire was used for the quantitative approach, and the qualitative aspect consisted of a desk review looking at key regulatory documents such as laws, regulations, policies, guidelines, standard operating procedures and reports. The data collection tool used was the WHO global benchmarking tool (GBT) for “Evaluation of National Regulatory System of Medical Product Version VI.
Results: The majority of the participants had a postgraduate degree (60%), and 72% had over 10 years of experience working at the NRA. Out of 251 sub-indicators assessed, 85 (34%) sub-indicators were fully implemented. Of the eight (8) functions assessed, sub-indicators related to clinical trial oversight and vigilance were the most common, with 67% and 62%, respectively. Of the 9 indicators assessed, 79% of the sub-indicators that are related to quality and risk management were implemented. The results of this study showed that PBSL operates at maturity level 1. The absence of laws and regulations that give PBSL the mandate to perform its regulatory functions was a major challenge even though other indicators were met. The study reported other challenges toward effective functioning, including but not limited to a lack of sufficient staff, weak enforcement of the sale of medicines and a poorly equipped quality control laboratory.
A newly isolated high-yield siderophores from Trichoderma viride XZ11-1 with high antifungal activity against Fusarium oxysporum and growth-promoting ability in bananas
Authors: Cui HaiYang , Cheng Qifeng , Jing Tao , Chen Yufeng , Li XiaoJuan , Zhang Miaoyi , Qi Dengfeng , Feng Junting , Vafadar Farinaz , Wei Yongzan , Li kai , Zhao Yankun , Zhou Dengbo , Wang Wei , Xie Jianghui 
Published: May 2024

Abstract

Background: Fusarium wilt of banana, a soil-borne disease caused by Fusarium oxysporum (f.sp.) cubense, particularly the Tropical Race 4 (Foc TR4), severely hinders the progress of the banana industry. Utilizing antagonistic microorganisms to suppress pathogen growth and enhance banana production is seen as a promising strategy for disease management.
Results: Strain XZ11-1, exhibiting the highest siderophore production ability, was isolated from the Qixianling Tropical Rainforest in Hainan province, China. Through a combination of colony morphology, physiological and biochemical analysis, as well as a dual-gene analysis of ITS4 and TEF1, the strain was identified as Trichoderma viride. Following optimization of fermentation conditions, strain XZ11-1 produced siderophores with a relative content of 79.45%, identified as both hydroxamate siderophores and carboxylate-type siderophores. Additionally, strain XZ11-1 demonstrated potent antifungal activity against 10 phytopathogenic fungi. Co-cultivation experiments revealed that under iron-limiting conditions, siderophores were the primary factor inhibiting the growth of Foc TR4. Notably, the siderophores produced by strain XZ11-1 exhibited a stimulating effect on maize seed germination and effectively colonized the roots of banana plants. Pot experiments further demonstrated that Trichoderma viride XZ11-1 could suppress the growth of Foc TR4 by competing for iron in the environment through siderophore mediation. Moreover, it could enhance iron absorption by banana plants by secreting siderophores to bind with Fe3+ in the environment.
Conclusions: Therefore, the high-yield siderophores produced by Trichoderma viride XZ11-1 represent a valuable microbial resource for controlling plant pathogenic fungi and promoting plant growth.
Prevalence, Classifications and Factors Associated With Sexual Dysfunction among Adult Cancer Patients on Chemotherapy at Uganda Cancer Institute, Mbarara
Authors: Oenga Joel Nyanchiri , Tamukong Robert , Isiiko John , Gibu Sibali Gidimali , Yadesa Tadele Mekuriya , Kule Gidion 
Published: May 2024

Abstract

Background: Sexual dysfunction is one of the social challenges that devastate many people among them cancer patients, but which is considered non-medical and often disregarded. Chemotherapeutics used among cancer patients are potential risk factors for development of SD and such understanding of these risk factors can lead to numerous interventions to bypass their effects on sexual life.
Objective: The goal of this study was to determine the prevalence, classifications and factors associated with sexual dysfunctions among cancer patients on chemotherapy.
Methods: A cross-sectional study was conducted among 214 cancer patients at the Mbarara Regional Referral Hospital in south western Uganda for a period of 3 months from August to October 2023. A systematic sampling technique was employed in the study; a questionnaire was used to collect patient data. Standardized female sexual function index and international index of erectile function tools were used to classify types of sexual dysfunctions among women and men respectively. Sexual dysfunction associated factors were analyzed by logistic regression using stata version 17.
Results: Two hundred and fourteen participants were enrolled 127 males and 87 females with a median age of 50 years; overall 90 (42.06%) of the patients, 68 (54.33 %) of males and 21 (24.14%) of females) experienced at least one domain of sexual dysfunction. At presentation majority of men 43 (33.86%) reported overall sexual dissatisfaction, while majority of the female 15 (18.39%) patients reported decreased sexual desire others being reduced arousal and vaginal pain. The multivariate logistic regression identified the independent risk factors for sexual dysfunctions: female gender (aOR 3.99, 95% CI 1.93-8.25; p value =0.001), Gastro intestinal cancers (aOR 3.46, 95% CI 1.34-8.93; p value =0.010) and anthracyclines (aOR 4.26, 95% CI 1.02, 17.76; p value=0.047) were statistically significant independent risk factors of sexual dysfunctions.
Conclusions: Our findings suggest that there is a high prevalence of sexual dysfunctions among cancer patients at the Mbarara Regional Referral Hospital. In male patients, the overall sexual dissatisfaction is the most prevalent while decreased sexual desire is prevalent in female. Routine screening of sexual functions should be encouraged among all patients receiving chemotherapies. Female patients, those diagnosed with gastro-intestinal cancers, and those on regimens containing anthracyclines should be more closely monitored for sexual dysfunction.