bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systematic Localization of the Arabidopsis Core Cell Cycle Proteins Reveals Novel Cell Division Complexes
Authors: Boruc Joanna , Mylle Evelien , Duda Maria , De Clercq Rebecca , Rombauts Stephane , Geelen Danny , Hilson Pierre , Inzeݩ Dirk , Van Damme Daniel , Russinova Eugenia 
Published: December 2009

AbstractCell division depends on the correct localization of the cyclin-dependent kinases that are regulated by phosphorylation, cyclin proteolysis, and protein-protein interactions. Although immunological assays can define cell cycle protein abundance and localization, they are not suitable for detecting the dynamic rearrangements of molecular components during cell division. Here, we applied an in vivo approach to trace the subcellular localization of 60 Arabidopsis (Arabidopsis thaliana) core cell cycle proteins fused to green fluorescent proteins during cell division in tobacco (Nicotiana tabacum) and Arabidopsis. Several cell cycle proteins showed a dynamic association with mitotic structures, such as condensed chromosomes and the preprophase band in both species, suggesting a strong conservation of targeting mechanisms. Furthermore, colocalized proteins were shown to bind in vivo, strengthening their localization-function connection. Thus, we identified unknown spatiotemporal territories where functional cell cycle protein interactions are most likely to occur.
Stimulation of Nonselective Amino Acid Export by Glutamine Dumper Proteins
Authors: Pratelli Reݩjane , Voll Lars M. , Horst Robin J. , Frommer Wolf B. , Pilot Guillaume 
Published: December 2009

Abstract
Phloem and xylem transport of amino acids involves two steps: export from one cell type to the apoplasm, and subsequent import into adjacent cells. High-affinity import is mediated by proton/amino acid cotransporters, while the mechanism of export remains unclear. Enhanced expression of the plant-specific type I membrane protein Glutamine Dumper1 (GDU1) has previously been shown to induce the secretion of glutamine from hydathodes and increased amino acid content in leaf apoplasm and xylem sap. In this work, tolerance to low concentrations of amino acids and transport analyses using radiolabeled amino acids demonstrate that net amino acid uptake is reduced in the glutamine-secreting GDU1 overexpressor gdu1-1D. The net uptake rate of phenylalanine decreased over time, and amino acid net efflux was increased in gdu1-1D compared with the wild type, indicating increased amino acid export from cells. Independence of the export from proton gradients and ATP suggests that overexpression of GDU1 affects a passive export system. Each of the seven Arabidopsis (Arabidopsis thaliana) GDU genes led to similar phenotypes, including increased efflux of a wide spectrum of amino acids. Differences in expression profiles and functional properties suggested that the GDU genes fulfill different roles in roots, vasculature, and reproductive organs. Taken together, the GDUs appear to stimulate amino acid export by activating nonselective amino acid facilitators.
The meaning of major depression in family life: the viewpoint of the ill parent
Authors: Ahlström Britt Hedman , Skärsäter Ingela , Danielson Ella 
Published: December 2009

Aim and objective. The aim was to elucidate the meaning of major depression in family life from the viewpoint of an ill parent.Background. Major depression according toDiagnostic and Statistical Manual of Mental Disordersis common and may appear repeatedly over several years, and affects family life. Depression in parents has a negative impact on family function and children’s health; however, studies regarding the deeper understanding of major depression in family life are lacking.Design. A qualitative explorative study using narrative interviews with eight parents who were identified with major depression.Methods. A phenomenological‐hermeneutic method of interpretation was used for analysing interview texts and included naïve understanding, a structural analysis where text was divided into meaning units, which were condensed and abstracted, and finally a comprehensive understanding.Result. Two themes were extracted: ‘to be afflicted in an almost unmanageable situation’ with sub‐themes ‘feeling hopelessly bad’, ‘being worthless’, ‘being unsatisfied’ and the theme ‘to reconcile oneself to the situation’ with sub‐themes ‘being active’, ‘being satisfied’ and ‘maintaining parenthood’.Conclusion. Comprehensive understanding revealed the parents’ simultaneous suffering and dignity in family life; suffering with serious lack of well‐being and health, destroyed self‐confidence and unhappiness, and dignity with strength, confidence and joy in children. The movement between suffering and dignity complicated family life. Dignity was threatened by the awareness that suffering in major depression was recurrent. Dignity had to be repeatedly restored for self and the family, and family dignity has to be restored before others outside the family circle.Relevance to clinical practice. A deeper understanding of the meaning of major depression in family life is helpful and for healthcare professionals to prevent individual and family suffering by assisting and preserving dignity.
Wise women: mentoring as relational learning in perinatal nursing practice
Authors: Ryan Annette , Goldberg Lisa , Evans Joan 
Published: December 2009

Aims and objectives. The focus of this paper is on one of four themes from a study exploring mentoring relationships between nurses in the intrapartum setting. The theme, relational learning, highlights how perinatal nurses engage with each other and engage with birthing women on a journey of learning in perinatal nursing practice.Background. Few studies have explored the contextual, lived experiences of informal mentoring relationships within nursing, particularly within perinatal nursing.Design. A qualitative feminist phenomenological study that considered a gender‐centred, embodied exploration of human lived experiences was conducted.Method. Five registered nurses practicing on a tertiary level labour and delivery unit in eastern Canada were purposefully recruited. Data were collected by phenomenological interviews, practice observations in the clinical setting and reflective journaling.Results. Four themes emerged through thematic analysis and researcher interpretation: the meaning of nurse‐to‐nurse mentoring,mentoring as relational learning, mentoring as embodied learning and a contextual understanding of nurse‐to‐nurse mentoring. Relational learning came to be understood through feminist phenomenological analysis, which revealed that expert perinatal nursing knowledge develops within positive mentoring relationships between perinatal nurses practicing with birthing women. This learning extends beyond tasks to a holistic understanding of clinical situations within specific health and social contexts. The mentor models positive perinatal nursing practices and creates a sense of enthusiasm that harnesses the raw passion new nurses often have for practice.Conclusion. The findings in this study aim to promote the understanding of the importance of relational, experiential learning for perinatal nurses’ professional development.Relevance to clinical practice. The results from this study will encourage nurses and nurse leaders to support mentoring by providing adequate resources and positive feedback for mentoring relationships. This will foster and sustain expert nurses to support novice nurses in perinatal practice. The findings also offer insight for perinatal practices beyond nursing, including midwifery.
Interprofessional education: the interface of nursing and social work
Authors: Chan Engle Angela , Chi Samantha Pang Mei , Ching Shirley , Lam Syrine KS 
Published: December 2009

Aims.  To examine the influence of interdisciplinary seminars on undergraduate nursing and social work students’ perceptions of their learning.Background.  Collaboration is considered to be important for health professionals in working towards good patient care, and interdisciplinary education is seen as one way of addressing this need for greater collaboration and team work. Today’s health professionals are dealing with an increasing number of older and chronically ill patients. The biopsychosocial dimensions inherent in such chronic illnesses bring about a closer working relationship between the nursing and social work professions to foster good patient care. No local research in Hong Kong, however, has looked specifically at how these two professions can develop their collaborative skills and qualities through interdisciplinary education.Design.  Mixed methods design.Method.  Data from questionnaires, videotape recordings of the sessions and follow‐up phone interviews were used for quantitative and qualitative analyses.Results.  The findings revealed three themes: an increased awareness of each other’s professional values and personal judgement, a recognition of each other’s disciplinary knowledge emphases and more, and an appreciation for, and learning about each other’s roles for future collaboration.Conclusions.  Whilst, it is usual to identify health professionals as non‐judgemental, it is also important to recognise the existence of their personal and professional values and beliefs that shape their decision‐making. Equally beneficial for students is their reported understanding of the other discipline’s emphasis on the physical or social aspects of care, and the interrelationships and complementary values that lead to students’ appreciation of each other’s roles and the possibility for their future collaboration in the holistic care of patients.Relevance to clinical practice.  The sharing of each other’s knowledge and their appreciation of the corresponding roles enhanced students’ decision‐making capacity and the extension of the holistic approach beyond one profession, which is essential for good patient care.
Family Strain Questionnaire – Short Form for nurses and general practitioners
Authors: Vidotto Giulio , Ferrario Silvia Rossi , Bond Trevor G , Zotti Anna Maria 
Published: December 2009

Aim.  Our paper presents an instrument for recognising the psychological problems of caregivers: the short form of a previously validated questionnaire, the Family Strain Questionnaire.Background.  Individuals caring for chronic patients frequently suffer from psychological problems, which are underestimated by medical professionals and, consequently, are not adequately treated. Therefore, clinical nurses, general practitioners and the members of home care teams have to recognise the psychological problems of caregivers. To do so, they need to quickly measure the problem’s severity quickly and track it over time, without a specific training in psychological diagnosis.Design.  Survey.Method.  Principal caregivers (n = 811) completed the Family Strain Questionnaire in its original form (semi‐structured interview plus 44 dichotomous items, administrable in about 20 minutes), aimed at investigating the subjective perception of emotional burden, problems in social involvement, the need for more information about the disease, satisfaction with family relationships and thoughts about death. Data were analysed using the Rasch model for the reduction of items. Thirty items were selected and reanalysed in a new sample of 40 caregivers.Results.  The thirty items selected showed very good fit statistics and maintained the diagnostic qualities of the original form. They thus compose the Family Strain Questionnaire ‐ Short Form that caregivers can complete in about five minutes. This brief measure can indicate the severity of stress and allows categorisation of caregivers by psychological risk, which is also represented by a graphic tool.Conclusion.  This study confirms that the Family Strain Questionnaire – Short Form offers an initial measure of caregivers’ psychological status, regardless of the patient’s disease and allows professionals to monitor it over time with a rapid and effective instrument.Relevance to clinical practice.  Clinical nurses, general practitioners and homecare teams should find the Family Strain Questionnaire – Short Form useful for assessing caregiving strain. The instrument is quick and easy to use and does not require any sophisticated psychological training.
An evidence‐based practice approach to improving nursing care of acute stroke in an Australian Emergency Department
Authors: Considine Julie , McGillivray Bree 
Published: December 2009

Aims.  The aim of this study was to improve the emergency nursing care of acute stroke by enhancing the use of evidence regarding prevention of early complications.Background.  Preventing complications in the first 24–48 hours decreases stroke‐related mortality. Many patients spend considerable part of the first 24 hours following stroke in the Emergency Department therefore emergency nurses play a key role in patient outcomes following stroke.Design.  A pre‐test/post‐test design was used and the study intervention was a guideline for Emergency Department nursing management of acute stroke.Methods.  The following outcomes were measured before and after guideline implementation: triage category, waiting time, Emergency Department length of stay, time to specialist assessment, assessment and monitoring of vital signs, temperature and blood glucose and venous‐thromboembolism and pressure injury risk assessment and interventions.Results.  There was significant improvement in triage decisions (21·4% increase in triage category 2, p = 0·009; 15·6% decrease in triage category 4, p = 0·048). Frequency of assessments of respiratory rate (p = 0·009), heart rate (p = 0·022), blood pressure (p = 0·032) and oxygen saturation (p = 0·001) increased. In terms of risk management, documentation of pressure area interventions increased by 28·8% (p = 0·006), documentation of nil orally status increased by 13·8% (ns), swallow assessment prior to oral intake increased by 41·3% (p = 0·003), speech pathology assessment in Emergency Department increased by 6·1% (ns) and there was 93·5 minute decrease in time to speech pathology assessment for admitted patients (ns).Relevance to clinical practice.  An evidence‐based guideline can improve emergency nursing care of acute stroke and optimise patient outcomes following stroke. As the continuum of stroke care begins in the Emergency Department, detailed recommendations for evidence‐based emergency nursing care should be included in all multidisciplinary guidelines for the management of acute stroke.
Assessing health‐related quality of life in patients with inflammatory bowel disease in Zhejiang, China
Authors: Zhou Yunxian , Ren Weihong , Irvine Elizabeth Jan , Yang Dagan 
Published: December 2009

Aims.  The aim of this study was to assess health‐related quality of life in patients with inflammatory bowel disease in Zhejiang, Mainland China.Background.  The incidence of inflammatory bowel disease in China is believed to be low but has been increasing in the past decade. The quality of life of Chinese patients with inflammatory bowel disease is unknown.Design.  A cross‐sectional study.Methods.  The study was conducted in 92 patients with inflammatory bowel disease in Zhejiang, China, 52 with ulcerative colitis and 40 with Crohn’s disease. Health‐related quality of life was measured by the Chinese version of the Inflammatory Bowel Disease Questionnaire and Short Form‐36, respectively. Disease activity was assessed by the Walmsley and Harvey–Bradshaw simple indices for ulcerative colitis and Crohn’s disease, respectively. Demographic and clinical variables were also recorded. Short Form‐36 data from the study sample were compared with a reference population of 1688 Chinese people residing in Hangzhou, Zhejiang, China.Results.  No significant health‐related quality of life differences were found between patients with ulcerative colitis and Crohn’s disease (p > 0·05). Pooled data showed that inflammatory bowel disease patients with active disease had significantly lower scores for all eight dimensions of Short Form‐36 compared to those in remission (p < 0·01); those with active disease scored significantly lower than population norms in all dimensions of Short Form‐36 except mental health (p < 0·05); whereas those in remission scored significantly lower than population norms in role physical (p < 0·01) and general health dimensions (p < 0·05). The regression analyses identified only disease activity index and employment status to explain variations in health‐related quality of life (p < 0·01).Conclusions.  Inflammatory bowel disease similarly impairs health‐related quality of life in patients with both ulcerative colitis and Crohn’s disease.Relevance to clinical practice.  The results suggest that any interventions that produce a stable clinical remission, whether medical or surgical, allowing patients to return to their usual work position can decrease the disease impact on their daily lives.
The effectiveness of multimedia learning education programs on knowledge, anxiety and pressure garment compliance in patients undergoing burns rehabilitation in Taiwan: an experimental study
Authors: Lo Shu‐Fen , Hayter Mark , Hsu Ma , Lin Su‐Er , Lin Shu‐In 
Published: December 2009

Aim.  This study examined the effectiveness of multimedia learning education programmes (MLEPs) on knowledge, anxiety and pressure garment compliance behaviour of burn patients.Background.  MLEPs are increasingly suggested as education methods that can be used by health professionals in daily practice. A systematic review of the effectiveness of computer‐based education demonstrates its acceptability to patients. However, few studies have empirically tested this approach.Design.  Experimental study.Methods.  An experimental study was conducted at a burn centre of a medical centre in Taipei, Taiwan. Participants were randomly assigned to either experimental (n = 28) or control groups (n = 30). Both groups were given pre‐tests and post‐tests to measure their knowledge, anxiety and pressure garment compliance behaviour.Results.  At two weeks, the results of this study showed that the MLEP had ‘statistically’ significantly improved patients’ burn knowledge, reduced individual anxiety and enhanced pressure garment compliance behaviour.Conclusion.  This controlled study demonstrated that a two week MLEP can be a useful intervention to improve care compliance and reduce anxiety in patients undergoing burns rehabilitation period.Relevance to clinical practice.  Burn injury patients undergoing rehabilitation are likely to benefit from multimedia learning education intervention programmes. More comprehensive health economic evaluation needs to be undertaken. Further studies exploring a longer term follow‐up are also required.
LLT1‐mediated Activation of IFN‐γ Production in Human Natural Killer Cells Involves ERK Signalling Pathway
Authors: Bambard N. D. , Mathew S. O. , Mathew P. A. 
Published: December 2009

AbstractNatural killer (NK) cell functions are regulated by a delicate balance of signals received through activating and inhibitory receptors expressed on the cell surface. Lectin‐like transcript‐1 (LLT1), expressed on a subpopulation of NK cells and other immune cells is a ligand for the NK cell inhibitory receptor, NKR‐P1A (CD161). Previous studies showed that cross‐linking surface LLT1 with a monoclonal antibody stimulated NK cell IFN‐γ secretion but had no effect on cytotoxicity. Here, we have examined the signalling pathways associated with LLT1‐stimulated IFN‐γ secretion. We ligated LLT1 on NK92 cells with CD161 on target cells and analysed IFN‐γ production in the presence of pharmacological inhibitors specific for various signalling mechanisms. These results indicate that LLT1 employs Src‐PTK, p38 and ERK signalling pathways, but not PKC, PI3K or calcineurin. Phosphorylation studies of the signalling adaptor molecules confirmed that the ERK signalling pathway is associated with LLT1‐mediated IFN‐γ production. LLT1 ligation is not associated with any change in detectable IFN‐γ mRNA levels suggesting that LLT1‐stimulated IFN‐γ production in NK cells may involve post‐transcriptional or translational events.
Cranial Muscles of the Sulawesi Babirusa (Babyrousa celebensis)
Authors: Kneepkens A. F. L. M. , Macdonald A. A. 
Published: December 2009

SummaryWith 12 figures and 1 tableThe detailed muscular anatomy of the head of the Sulawesi Babirusa (Babyrousa celebensis) is described for the first time. The results show that the muscular anatomy of the Babyrousa is very similar to the pig genus Sus, despite long geological separation from it. Some differences were noted: the M. parietoauricularis was more clearly separated into two parts in the Babirusa than in the domestic pig; the rostral fibres of the M. levator anguli oculi reached the infraorbital sinus in the Babirusa but only as far as the medial corner of the eye in the domestic pig; the M. palatinus of the Babirusa is paired and did not reach the Os palatinum, unlike its description in the domestic pig and the Platysma pars zygomatica originates from the fascia of the neck in the Babirusa, whereas that of the domestic pig originates largely or entirely from the scapula.
The use of intravenous immunoglobulin for treatment of dermatological conditions in Australia: A review
Authors: Smith Saxon D , Dennington Peta M , Cooper Alan 
Published: December 2009

ABSTRACTIntravenous immunoglobulin has been used in the treatment of various dermatological conditions, including toxic epidermal necrolysis, bullous pemphigoid and pemphigus vulgaris. From March 2008, the National Blood Authority has implemented the ‘Criteria for the clinical use of intravenous immunoglobulins in Australia’. The new criteria have formalized the eligibility requirements for several dermatological conditions. This may increase access to intravenous immunoglobulin for treatment for these conditions. However, there remain stringent eligibility criteria with which dermatologists need to be acquainted. In some conditions, dermatology review is mandated by these criteria while in other conditions with skin manifestations, referral to other specialists is required. The following article provides a summary of the salient points in relation to the clinical use of intravenous immunoglobulin in dermatology, as well as its production and supply in Australia.
How to get the most from the medical literature: Searching the medical literature effectively
Authors: WEBSTER ANGELA C , CROSS NICHOLAS B , MITCHELL RUTH , CRAIG JONATHAN C 
Published: December 2009

ABSTRACT:Different clinical questions are best answered using different study designs. This paper describes the best methods for finding relevant studies for well‐framed clinical questions. We focus on which database is best to search to answer your question, describe the structure of effective search strategies and explore ways to develop appropriate search terms. We illustrate these with sensitive and specific search strategies to answer different clinical questions arising from a hypothetical clinical scenario typical of a nephrologist's everyday practice.
HOUSING POLICY FOR THE ELDERLY: A POLICY TO BUILD BARRIER‐FREE RENTAL HOUSING
Authors: Hirono Keiko Nosse 
Published: December 2009

AbstractThere is a strong need for barrier‐free rental housing because of the rapidly aging population in Japan, where the share of barrier‐free rental housing is only 18.24% of total rental housing. We propose a policy to solve the problems associated with supplying barrier‐free rental housing. Our scheme involves a method of funding rental housing with a securities investor, as well as implementing property management and introducing rent subsidies. The plan entails funds being collected to construct barrier‐free rental housing. The housing will generate positive rental profits, which will, in turn, lead to the increase in the supply of barrier‐free rental housing. According to our plan, the low‐income elderly will also have access to barrier‐free rental housing.