bluuebunny/crossref_metadata_2025_split huggingface dataset:
Opposing function of MYBBP1A in proliferation and migration of head and neck squamous cell carcinoma cells
Authors: Acuña Sanhueza Gustavo A , Faller Leonie , George Babitha , Koffler Jennifer , Misetic Vinko , Flechtenmacher Christa , Dyckhoff Gerhard , Plinkert Peter P , Angel Peter , Simon Christian , Hess Jochen 
Published: February 2012

AbstractBackgroundHead and neck squamous cell carcinoma (HNSCC) is one of the most prevalent and lethal cancers worldwide and mortality mostly results from loco-regional recurrence and metastasis. Despite its significance, our knowledge on molecular, cellular and environmental mechanisms that drive disease pathogenesis remains largely elusive, and there are limited therapeutic options, with only negligible clinical benefit.MethodsWe applied global gene expression profiling with samples derived from a recently established mouse model for oral cancer recurrence and identified a list of genes with differential expression between primary and recurrent tumors.ResultsOne differentially expressed gene codes for Myb-binding protein 1a (MYBBP1A), which is known as a transcriptional co-regulator that physically interacts with nuclear transcription factors, such as NFκB and p53. We confirmed significantly reduced MYBBP1A protein levels on tissue sections of recurrent mouse tumors compared to primary tumors by immunohistochemistry, and found aberrant MYBBP1A protein levels also in tumor samples of HNSCC patients. Interestingly, silencing of MYBBP1A expression in murine SCC7 and in human HNSCC cell lines elicited increased migration but decreased cell growth.ConclusionWe provide experimental evidence that MYBBP1A is an important molecular switch in the regulation of tumor cell proliferation versus migration in HNSCC and it will be a major challenge for the future to proof the concept whether regulation MYBBP1A expression and/or function could serve as a novel option for anti-cancer therapy.
Societal and individual burden of illness among fibromyalgia patients in France: Association between disease severity and OMERACT core domains
Authors: Perrot Serge , Schaefer Caroline , Knight Tyler , Hufstader Meghan , Chandran Arthi B , Zlateva Gergana 
Published: February 2012

Abstract

Background
Patients with fibromyalgia (FM) report widespread pain, fatigue, and other functional limitations. This study aimed to provide an assessment of the burden of illness associated with FM in France and its association with disease severity and core domains as defined by Outcome Measures in Rheumatology Clinical Trials (OMERACT) for FM.

Methods
This cross-sectional, observational study recruited patients with a prior diagnosis of FM from 18 community-based physician offices in France. Patients completed questions about FM impact (Fibromyalgia-Impact Questionnaire [FIQ]), core symptoms (defined by OMERACT), health-related quality of life (EQ-5D), current overall health status (rated on a scale from 0 to 100), productivity, treatment satisfaction, and out-of-pocket expenses related to FM. Site staff recorded patients' treatment and health resource use based on medical record review. Costs were extrapolated from 4-week patient-reported data and 3-month clinical case report form data and calculated in 2008 Euros using a societal perspective. Tests of significance used the Kruskal-Wallis test or Fisher's Exact test where P < 0.05 was considered significant.

Results
Eighty-eight patients (mean 55.2 y; female:male 74:14) were recruited. The majority of patients (84.1%) were prescribed medications for FM. Patients mainly described medications as a little/not at all effective (40.0%) or somewhat effective (52.9%). Current Overall Health rating was 52.9 (± 17.8) and FIQ total score was 54.8 (± 17.3). FIQ total score was used to define FM severity, and 17 patients scored 0- < 39 (mild FM), 33 patients 39- < 59 (moderate FM), and 38 scored 59-100 (severe FM). As FM severity level worsened, patients had poorer overall health status and perceived their prescription medications to be less effective. Average cost/FM patient was higher for severe (€10,087) vs. moderate (€6,633) or mild FM (€5,473); however, the difference was not significant.

Conclusions
In a sample of 88 patients with FM from France, we found that FM poses a substantial economic and human burden on patients and society. FM severity level was significantly associated with patients' health status and core symptom domains.
Sendai virus-based liposomes enable targeted cytosolic delivery of nanoparticles in brain tumor-derived cells
Authors: Dudu Veronica , Rotari Veronica , Vazquez Maribel 
Published: February 2012

ABSTRACTBackgroundNanotechnology-based bioassays that detect the presence and/or absence of a combination of cell markers are increasingly used to identify stem or progenitor cells, assess cell heterogeneity, and evaluate tumor malignancy and/or chemoresistance. Delivery methods that enable nanoparticles to rapidly detect emerging, intracellular markers within cell clusters of biopsies will greatly aid in tumor characterization, analysis of functional state and development of treatment regimens.ResultsExperiments utilized the Sendai virus to achievein vitro, cytosolic delivery of Quantum dots in cells cultured from Human brain tumors. Using fluorescence microscopy and Transmission Electron Microscopy,in vitroexperiments illustrated that these virus-based liposomes decreased the amount of non-specifically endocytosed nanoparticles by 50% in the Human glioblastoma and medulloblastoma samples studied. Significantly, virus-based liposome delivery also facilitated targeted binding of Quantum dots to cytosolic Epidermal Growth Factor Receptor within cultured cells, focal to the early detection and characterization of malignant brain tumors.ConclusionsThese findings are the first to utilize the Sendai virus to achieve cytosolic, targeted intracellular binding of Qdots within Human brain tumor cells. The results are significant to the continued applicability of nanoparticles used for the molecular labeling of cancer cells to determine tumor heterogeneity, grade, and chemotherapeutic resistivity.
Strong, conductive carbon nanotube fibers as efficient hole collectors
Authors: Jia Yi , Li Xiao , Li Peixu , Wang Kunlin , Cao Anyuan , Wei Jinquan , Zhu Hongwei , Wu Dehai 
Published: February 2012

Abstract
We present the photovoltaic properties of heterojunctions made from single-walled carbon nanotube (SWNT) fibers and n-type silicon wafers. The use of the opaque SWNT fiber allows photo-generated holes to transport along the axis direction of the fiber. The heterojunction solar cells show conversion efficiencies of up to 3.1% (actual) and 10.6% (nominal) at AM1.5 condition. In addition, the use of strong, environmentally benign carbon nanotube fibers provides excellent structural stability of the photovoltaic devices.
Expression analysis of LIM gene family in poplar, toward an updated phylogenetic classification
Authors: Arnaud Dominique , Déjardin Annabelle , Leplé Jean-Charles , Lesage-Descauses Marie-Claude , Boizot Nathalie , Villar Marc , Bénédetti Hélène , Pilate Gilles 
Published: February 2012

AbstractBackgroundPlant LIM domain proteins may act as transcriptional activators of lignin biosynthesis and/or as actin binding and bundling proteins. PlantLIMgenes have evolved in phylogenetic subgroups differing in their expression profiles: in the whole plant or specifically in pollen. However, several poplarPtLIMgenes belong to uncharacterized monophyletic subgroups and the expression patterns of theLIMgene family in a woody plant have not been studied.FindingsIn this work, the expression pattern of the twelve duplicated poplarPtLIMgenes has been investigated by semi quantitative RT-PCR in different vegetative and reproductive tissues. As in other plant species, poplarPtLIMgenes were widely expressed in the tree or in particular tissues. Especially,PtXLIM1a, PtXLIM1bandPtWLIM1bgenes were preferentially expressed in the secondary xylem, suggesting a specific function in wood formation. Moreover, the expression of these genes and of thePtPLIM2agene was increased in tension wood. Western-blot analysis confirmed the preferential expression of PtXLIM1a protein during xylem differentiation and tension wood formation. Genes classified within the pollen specific PLIM2 and PLIM2-like subgroups were all strongly expressed in pollen but also in cottony hairs. Interestingly, pairs of duplicatedPtLIMgenes exhibited different expression patterns indicating subfunctionalisations in specific tissues.ConclusionsThe strong expression of severalLIMgenes in cottony hairs and germinating pollen, as well as in xylem fibers suggests an involvement of plant LIM domain proteins in the control of cell expansion. Comparisons of expression profiles of poplarLIMgenes with the published functions of closely related plantLIMgenes suggest conserved functions in the areas of lignin biosynthesis, pollen tube growth and mechanical stress response. Based on these results, we propose a novel nomenclature of poplar LIM domain proteins.
In-Silico screening of Pleconaril and its novel substituted derivatives with Neuraminidase of H1N1 Influenza strain
Authors: Hussain Basha Syed , Prasad R Nalini 
Published: February 2012

AbstractBackgroundNeuraminidase (NA) is a prominent surface antigen of Influenza viruses, which helps in release of viruses from the host cells after replication. Anti influenza drugs such as Oseltamivir target a highly conserved active site of NA, which comprises of 8 functional residues (R118, D151, R152, R224, E276, R292, R371 and Y406) to restrict viral release from host cells, thus inhibiting its ability to cleave sialic acid residues on the cell membrane. Reports on the emergence of Oseltamivir resistant strains of H1N1 Influenza virus necessitated a search for alternative drug candidates. Pleconaril is a novel antiviral drug being developed by Schering-Plough to treat Picornaviridae infections, and is in its late clinical trials stage. Since, Pleconaril was designed to bind the highly conserved hydrophobic binding site on VP1 protein of Picorna viruses, the ability of Pleconaril and its novel substituted derivatives to bind highly conserved hydrophobic active site of H1N1 Neuraminidase, targeting which oseltamivir has been designed was investigated.Result310 novel substituted variants of Pleconaril were designed using Chemsketch software and docked into the highly conserved active site of NA using arguslab software. 198 out of 310 Pleconaril variants analyzed for docking with NA active site were proven effective, based on their free binding energy.ConclusionPleconaril variants with F, Cl, Br, CH3, OH and aromatic ring substitutions were shown to be effective alternatives to Oseltamivir as anti influenza drugs.
Classification of the height and flexibility of the medial longitudinal arch of the foot
Authors: Nilsson Mette Kjærgaard , Friis Rikke , Michaelsen Maria Skjoldahl , Jakobsen Patrick Abildgaard , Nielsen Rasmus Oestergaard 
Published: February 2012

AbstractBackgroundThe risk of developing injuries during standing work may vary between persons with different foot types. High arched and low arched feet, as well as rigid and flexible feet, are considered to have different injury profiles, while those with normal arches may sustain fewer injuries. However, the cut‐off values for maximum values (subtalar position during weight‐bearing) and range of motion (ROM) values (difference between subtalar neutral and subtalar resting position in a weight‐bearing condition) for the medial longitudinal arch (MLA) are largely unknown. The purpose of this study was to identify cut‐off values for maximum values and ROM of the MLA of the foot during static tests and to identify factors influencing foot posture.MethodsThe participants consisted of 254 volunteers from Central and Northern Denmark (198 m/56 f; age 39.0 ± 11.7 years; BMI 27.3 ± 4.7 kg/m2). Navicular height (NH), longitudinal arch angle (LAA) and Feiss line (FL) were measured for either the left or the right foot in a subtalar neutral position and subtalar resting position. Maximum values and ROM were calculated for each test. The 95% and 68% prediction intervals were used as cut‐off limits. Multiple regression analysis was used to detect influencing factors on foot posture.ResultsThe 68% cut‐off values for maximum MLA values and MLA ROM for NH were 3.6 to 5.5 cm and 0.6 to 1.8 cm, respectively, without taking into account the influence of other variables. Normal maximum LAA values were between 131 and 152° and normal LAA ROM was between ‐1 and 13°. Normal maximum FL values were between ‐2.6 and ‐1.2 cm and normal FL ROM was between ‐0.1 and 0.9 cm. Results from the multivariate linear regression revealed an association between foot size with FL, LAA, and navicular drop.ConclusionsThe cut‐off values presented in this study can be used to categorize people performing standing work into groups of different foot arch types. The results of this study are important for investigating a possible link between arch height and arch movement and the development of injuries.
A clinician‐nurse model to reduce early mortality and increase clinic retention among high‐risk HIV‐infected patients initiating combination antiretroviral treatment
Authors: Braitstein Paula , Siika Abraham , Hogan Joseph , Kosgei Rose , Sang Edwin , Sidle John , Wools‐Kaloustian Kara , Keter Alfred , Mamlin Joseph , Kimaiyo Sylvester 
Published: February 2012

BackgroundIn resource‐poor settings, mortality is at its highest during the first 3 months after combination antiretroviral treatment (cART) initiation. A clear predictor of mortality during this period is having a low CD4 count at the time of treatment initiation. The objective of this study was to evaluate the effect on survival and clinic retention of a nurse‐based rapid assessment clinic for high‐risk individuals initiating cART in a resource‐constrained setting.MethodsThe USAID‐AMPATH Partnership has enrolled more than 140,000 patients at 25 clinics throughout western Kenya. High Risk Express Care (HREC) provides weekly or bi‐weekly rapid contacts with nurses for individuals initiating cART with CD4 counts of ≤100 cells/mm3. All HIV‐infected individuals aged 14 years or older initiating cART with CD4 counts of ≤100 cells/mm3 were eligible for enrolment into HREC and for analysis. Adjusted hazard ratios (AHRs) control for potential confounding using propensity score methods.ResultsBetween March 2007 and March 2009, 4,958 patients initiated cART with CD4 counts of ≤100 cells/mm3. After adjusting for age, sex, CD4 count, use of cotrimoxazole, treatment for tuberculosis, travel time to clinic and type of clinic, individuals in HREC had reduced mortality (AHR: 0.59; 95% confidence interval: 0.45‐0.77), and reduced loss to follow up (AHR: 0.62; 95% CI: 0.55‐0.70) compared with individuals in routine care. Overall, patients in HREC were much more likely to be alive and in care after a median of nearly 11 months of follow up (AHR: 0.62; 95% CI: 0.57‐0.67).ConclusionsFrequent monitoring by dedicated nurses in the early months of cART can significantly reduce mortality and loss to follow up among high‐risk patients initiating treatment in resource‐constrained settings.
Increased expression of hypoxia-inducible factor-1α and metallothionein in varicocele and varicose veins
Authors: Lee J-D , Lai C-H , Yang W-K , Lee T-H 
Published: February 2012

Objective The increased blood stasis and venous volume pressure causing tissue hypoxia are observed in both varicocele and varicose veins. Metallothionein (MT), a metal-binding protein, protects against cell apoptosis under hypoxic stress. It also plays an important role in collateral flow recovery and angiogenesis. We studied the distribution of hypoxia-inducible factor-1α (HIF-1α) and MT in varicocele and varicose veins. Methods The study specimens consisted of 1 cm venous segments that were obtained from 12 male patients during vascular stripping surgery for varicose veins and 1 cm of internal spermatic vein (ISV) obtained from 12 patients during left varicocele repair. The control samples of 1 cm ISV were obtained from 10 male patients who underwent left inguinal herniorrhaphy. All vascular specimens were analysed for HIF-1α and MT expression by immunoblotting, immunohistochemical (IHC) staining and confocal microscopy. Data were analysed using one-way analysis of variance with Tukey's comparison test. Results In both venous diseases, the increased expression of HIF-1α and MT compared with the control group ( P < 0.05) and most of the proteins distributed over smooth muscle layers were detected by IHC staining; HIF-1α and MT in the muscle layer with co-localization, and MT overexpression especially located in the endothelium of both venous diseases under confocal microscopy. Conclusions Our results revealed the higher expression of HIF-1α and MT in varicocele and varicose veins than in the control group; MT overexpression in the muscle layer of both diseased vessels and especially located in the endothelium under confocal microscopy. MT has the function to protect vascular cells from apoptosis under hypoxia. Thus, this MT function may cause a decreased vascular cell apoptosis and then contribute to the dilated and thickened walls of varicocele and varicose veins.
Reflux patterns and risk factors of primary varicose veins’ clinical severity
Authors: García-Gimeno M , Rodríguez-Camarero S , Tagarro-Villalba S , Ramalle-Gomara E , García J A Ajona , Arranz M A González , García D López , González-González E , Puerta C Vaquero 
Published: February 2012

Objectives Primarily, to determine the association between the clinical severity of primary varicose veins and different reflux patterns in an anatomic and haemodynamic clinical study using duplex ultrasonography (DU). Secondly, to analyse the association of clinical severity with other aspects, such as risk factors for chronic venous insufficiency (CVI) and other concurrent diseases. Method A total of 2036 limbs were evaluated using DU. Clinical status was characterized by the CEAP (clinical, aetiological, anatomical and pathological elements) classification. The degree of clinical severity was grouped into two categories, mild to moderate CVI (C1–C3) and severe CVI, characterized by the presence of skin changes (C4–C6). We analysed the association of the different reflux patterns with CEAP status. Results Saphenofemoral junction (SFJ) reflux of the great saphenous vein (GSV) was associated with the most severe form of the disease (odds ratio [OR] = 2.96; confidence interval [CI] 95%: 2.2–3.8), whereas competent SFJ of the GSV with reflux from proximal veins (OR = 2; CI 95%: 1.4 –2.7) and the pure non-saphenous reflux (OR = 4.1; CI 95%: 1.8–9.0) were associated with mild to moderate CVI. Obesity increased the frequency of severe CVI 2.7 times (OR = 2.7; CI 95%: 1.6–4.6); being a woman also increased the frequency of more severe disease 1.3 times (OR = 1.3; CI 95%: 1.0–1.7). Conclusion Anatomical and haemodynamic studies by DU are postulated as a useful diagnostic tool that allow, by identifying the pattern of venous reflux of varicose pathology, characterization of the probable association to CVI clinical severity.
A Mixed Valent Thioantimonate(III,V): [SbS3]3– and [SbS4]3– as bidentate ligands: Solvothermal synthesis and crystal structure of {Mn(dien)2}2[MnSb2S7]
Authors: Herzberg Nicola , Näther Christian , Bensch Wolfgang 
Published: February 2012

Abstract
The new compound {Mn(dien)2}2[MnSb2S7] (dien = diethylenetriamine) was obtained under solvothermal conditions and crystallizes in the orthorhombic space group Pbca. The main structural motif is the [MnSb2S7]4– anion being constructed by a [MnS4]6– tetrahedron, a [SbIIIS3]3– and a [SbVS4]3– anion. Each thioantimonate anion shares one edge with the Mn2+ centered cation generating the [MnSb2S7]4– unit. The two independent Mn2+ cations in the two {Mn(dien)2}2+ complexes are in a distorted octahedral environment of two tridentate acting dien molecules. One cation displays the λδ–λλ-conformation and the other the δλ–λδ-conformation. Several intermolecular S···H—N bonding interactions are found between the cations and the anions.
Crystal structure determination of Hägg carbide, χ-Fe5C2 by first-principles calculations and Rietveld refinement
Authors: Leineweber Andreas , Shang Shunli , Liu Zi-Kui , Widenmeyer Marc , Niewa Rainer 
Published: February 2012

Abstract
X-ray powder-diffraction data recorded using different wave lengths as well as neutron powder diffraction data on Hägg carbide, χ-Fe5C2, were evaluated by Rietveld or Pawley refinements, respectively. Likewise, employing different starting models, first-principles calculations using density functional theory (DFT) involving structure optimisation with respect to energy were performed for χ-Fe5C2. The results from diffraction and DFT imply a crystal structure having a monoclinic C2/c symmetry with a quite regular (monocapped) trigonal-prismatic coordination of C by Fe atoms. The anisotropy of the microstrain broadening observed in the powder-diffraction patterns agrees with the anisotropy of the reciprocal Young’s module obtained from elastic constants calculated by DFT. The anisotropic microstrain broadening can to some degree, be modelled allowing for a triclinic distortion of the metric of χ-Fe5C2 (deviation of the lattice angle γ from 90°) involving reflection spitting, which mimics the hkl-dependently broadened reflections. This distortion corresponds to the most compliant shear direction of the monoclinic χ-Fe5C2. The anisotropic microstrain broadening results from microstress induced e.g. by anisotropic thermal expansion inducing misfit between the grains, in association with the intrinsic anisotropic elastic compliance of χ-Fe5C2. This anisotropic microstrain broadening was likely the origin of previous proposals of triclinic P-1 space-group symmetry for the crystal structure of χ-Fe5C2, which is rejected in the present work.
The use of periodic graphs for representing and analysing crystal structures
Authors: Klein Hans-Joachim 
Published: February 2012

Abstract
Several graph forms are discussed which are suited for modelling crystal structures at different levels of detail. Graphs with nodes representing atoms or special views of coordination polyhedra and edges representing bonds or different forms of connections between polyhedra are considered. Space-group symmetries are used to fold infinite graphs to finite representations which allow to develop algorithms for computing properties of structures effectively. Some applications are presented which demonstrate the usage of the graph forms.