bluuebunny/crossref_metadata_2025_split huggingface dataset:
Utilizing collagen membranes for guided tissue regeneration‐based root coverage
Authors: Wang Hom‐Lay , Modarressi Marmar , Fu Jia‐Hui 
Published: April 2012

AbstractGingival recession is a common clinical problem that can result in hypersensitivity, pain, root caries and esthetic concerns. Conventional soft tissue procedures for root coverage require an additional surgical site, thereby causing additional trauma and donor site morbidity. In addition, the grafted tissues heal by repair, with formation of long junctional epithelium with some connective tissue attachment. Guided tissue regeneration‐based root coverage was thus developed in an attempt to overcome these limitations while providing comparable clinical results. This paper addresses the biologic foundation of guided tissue regeneration‐based root coverage, and describes the indications and contraindications for this technique, as well as the factors that influence outcomes. The step‐by‐step clinical techniques utilizing collagen membranes are also described. In comparison with conventional soft tissue procedures, the benefits of guided tissue regeneration‐based root coverage procedures include new attachment formation, elimination of donor site morbidity, less chair‐time, and unlimited availability and uniform thickness of the product. Collagen membranes, in particular, benefit from product biocompatibility with the host, while promoting chemotaxis, hemostasis, and exchange of gas and nutrients. Such characteristics lead to better wound healing by promoting primary wound coverage, angiogenesis, space creation and maintenance, and clot stability. In conclusion, collagen membranes are a reliable alternative for use in root coverage procedures.
Clinical utility of stem cells for periodontal regeneration
Authors: Hynes Kim , Menicanin Danijela , Gronthos Stan , Bartold P. Mark 
Published: April 2012

AbstractThe aim of this review is to discuss the clinical utility of stem cells in periodontal regeneration by reviewing relevant literature that assesses the periodontal‐regenerative potential of stem cells. We considered and described the main stem cell populations that have been utilized with regard to periodontal regeneration, including bone marrow‐derived mesenchymal stem cells and the main dental‐derived mesenchymal stem cell populations: periodontal ligament stem cells, dental pulp stem cells, stem cells from human exfoliated deciduous teeth, stem cells from apical papilla and dental follicle precursor cells. Research into the use of stem cells for tissue regeneration has the potential to significantly influence periodontal treatment strategies in the future.
Critical issues in clinical periodontal research
Authors: Preshaw Philip M. 
Published: April 2012

AbstractAs periodontal researchers and clinicians, we are challenged every day to make decisions relating to the clinical management of our patients and about how best to conduct clinical periodontal research. This volume of Periodontology 2000 addresses some of the critical issues in contemporary clinical periodontics and periodontal research that are of direct relevance to clinicians, researchers, teachers and students. The 11 review articles in this volume of Periodontology 2000 focus on aspects of periodontal research methodology and clinical periodontology. In terms of research methodology, the articles aim to inform the reader on topics relating to randomized controlled trials in periodontal research, evidence‐based dentistry, calibration of clinical examiners and statistics relevant to periodontal research. The clinical periodontology articles address issues relating to decisions on retaining periodontally compromised teeth or replacing them with implants, periodontal management in the patient with osteoporosis, surgical approaches for root coverage and the emerging science of advanced regenerative technologies, including the use of stem cells, for periodontal regeneration. It is hoped that these critical reviews will address many of the dilemmas that confront us on a regular basis and provide practical guidance to those engaged in both clinical periodontology and clinical periodontal research.
The Interaction Between Importin‐α and Nup153 Promotes Importin‐α/β‐Mediated Nuclear Import
Authors: Ogawa Yutaka , Miyamoto Yoichi , Oka Masahiro , Yoneda Yoshihiro 
Published: April 2012

AbstractNuclear transport is mediated by transport factors, including the importin β family members. The directionality of nuclear transport is governed by the asymmetrical distribution of the small GTPase Ran. Of note, importin α/β‐mediated import of classical nuclear localization signal (cNLS) – containing cargo is more efficient than other Ran‐dependent import pathways that do not require importin α. In this study, we characterized the role of importin α in nuclear transport by examining import efficiencies of cNLS‐cargo/importin α/β complexes. We first depleted digitonin‐permeabilized semi‐intact cells of endogenous importin α and used the cells to show that the interaction between importin α and Nup153 – a component of the nuclear pore complex (NPC) – is essential for efficient import of importin β‐binding domain containing substrates, but not other cargoes that directly bind to importin β. Moreover, we found that the binding of importin α to Nup153 facilitates cNLS‐mediated import, and demonstrated that importin α in import complexes and cargo‐free importin α prebound to Nup153 promote efficient import of cNLS‐containing proteins. This is the first in vitro study showing that in conjunction with Nup153, importin α contributes to directionally biased exit of cNLS‐containing cargo to the nuclear side of NPCs.
Treatment protocol for denture stomatitis, prior to anatomical molding
Authors: Milton Rocha Gusmão João , Pereira Renato Piai 
Published: April 2012

doi: 10.1111/j.1741‐2358.2012.00661.x

Treatment protocol for denture stomatitis, prior to anatomical molding
Background:  Microorganisms of the genus Candida have been recovered from complete dentures made of acrylic resin, with high numbers of colony forming units and species diversity, which can act as infectious agents causing chronic atrophic candidiasis (denture stomatitis).Objective:  The objective of this paper is present a treatment protocol for chronic atrophic candidiasis (denture stomatitis).Materials and Methods:  The work describes three cases of totally edentulous patients presenting palate stomatitis who were submitted for treatment associating denture rebasing with chemically‐activated acrylic resin, night immersion in 2.5% sodium hypochlorite and use of topical antifungals for two weeks.Results:  In all cases, remission of the inflammatory process occurred.Conclusion:  The proposed treatment protocol proved to be to be effective.
The Role of Left Superior Parietal Lobe in Male Sexual Behavior: Dynamics of Distinct Components Revealed by fMRI
Authors: Cera Nicoletta , Di Pierro Ezio D. , Sepede Gianna , Gambi Francesco , Perrucci Mauro Gianni , Merla Arcangelo , Tartaro Armando , Del Gratta Cosimo , Galatioto Paradiso Giuseppe , Vicentini Carlo , Romani Gian Luca , Ferretti Antonio 
Published: April 2012

ABSTRACT

Introduction
Despite the interest for the brain correlates of male sexual arousal, few studies investigated neural mechanisms underlying psychogenic erectile dysfunction (ED). Although these studies showed several brain regions active in ED patients during visual erotic stimulation, the dynamics of inhibition of sexual response is still unclear.

Aim
This study investigated the dynamics of brain regions involved in the psychogenic ED.

Methods
Functional magnetic resonance imaging (fMRI) and simultaneous penile tumescence (PT) were used to study brain activity evoked in 17 outpatients with psychogenic ED and 19 healthy controls during visual erotic stimulation. Patterns of brain activation related to different phases of sexual response in the two groups were compared.

Main Outcome Measures
Simultaneous recording of blood oxygen level-dependent fMRI responses and PT during visual erotic stimulation.

Results
During visual erotic stimuli, a larger activation was observed for the patient group in the left superior parietal lobe, ventromedial prefrontal cortex, and posterior cingulate cortex, whereas the control group showed larger activation in the right middle insula and dorsal anterior cingulate cortex and hippocampus. Moreover, the left superior parietal lobe showed a larger activation in patients than controls especially during the later stage of sexual response.

Conclusions
Our results suggest that, among regions more active in patient group, the left superior parietal lobe plays a crucial role in inhibition of sexual response. Previous studies showed that left superior parietal lobe is involved in monitoring of internal body representation. The larger activation of this region in patients during later stages of sexual response suggests a high monitoring of the internal body representation, possibly affecting the behavioral response. These findings provide insight on brain mechanisms involved in psychogenic ED.
Sleep duration and overweight/obesity in children: Review and implications for pediatric nursing
Authors: Liu Jianghong , Zhang Angelina , Li Linda 
Published: April 2012

AbstractPurpose.  The purpose of this study was to review evidence from the last 5 years (2006–2011) regarding a relationship between sleep duration and childhood overweight/obesity.Conclusions.  Among the 25 studies selected from PubMed and Web of Knowledge databases, all indicated significant associations between short sleep duration and childhood overweight/obesity. Studies explored a range of pediatric populations, methodologies, and potential contributing factors.Practice Implications.  Childhood sleep duration may be a modifiable risk factor in preventing obesity. In addition to identifying and assessing patients' sleep habits, nurses play important roles as educators regarding the importance of adequate sleep and promoting it in children.
PASTEURIZED BLUEBERRY (VACCINIUM CORYMBOSUM) JUICE INHIBITS GROWTH OF BACTERIAL PATHOGENS IN MILK BUT ALLOWS SURVIVAL OF PROBIOTIC BACTERIA
Authors: BISWAS DEBABRATA , WIDEMAN NATHAN E. , O'BRYAN CORLISS A. , MUTHAIYAN ARUNACHALAM , LINGBECK JODY M. , CRANDALL PHILIP G. , RICKE STEVEN C. 
Published: April 2012

ABSTRACTCompounds found in berries exhibit a wide range of effects including antimicrobial activities. In this study, the antimicrobial properties of blueberry juice against the foodborne pathogens Salmonella Typhimurium, Campylobacter jejuni, Listeria monocytogenes and enterohemorrhagic Escherichia coli O157:H7 were investigated. Inhibition was measured in 100% blueberry juice or a 1:1 (v/v) mixture of milk and blueberry juice with growth in 100% milk used as a control. The growth of all four pathogens was reduced below the detection level (100 cfu/mL) by blueberry juice, and reduced by 4–7 log cfu/mL in a mixture of milk and blueberry juice. The effects of blueberry juice on probiotic bacteria Lactobacillus bulgaricus and Bifidibacterium bifidis were also investigated. The growth was less in a 1:1 (v/v) mixture of blueberry juice and skim milk as compared with milk alone, but these probiotic organisms were able to survive and grow. These data demonstrate the potential of blueberry juice for use as an all‐natural antimicrobial.PRACTICAL APPLICATIONSFoodborne pathogens sicken millions in the U.S.A. each year. The research reported here indicates that juice from blueberries can inhibit the growth of these foodborne bacteria. In addition, the blueberry juice did not substantially inhibit the growth of beneficial bacteria such as those found in yogurt.
EFFECTS OF DIFFERENT CULTIVATION CONDITIONS ON STAPHYLOCOCCUS AUREUS BIOFILM FORMATION AND DIVERSITY OF ADHESIN GENES
Authors: TANG JUNNI , CHEN JUAN , LIU JI , ZHANG RONG , YANG RONGSHENG , CHEN LIANGHONG 
Published: April 2012

ABSTRACTStaphylococcus aureus isolates from foodborne outbreaks were screened for the presence of icaAD, icaBC, clfB, clfA, fnbA, fnbB, ebpS, eno, fib, sasG, sasC, bbp, bap and cna genes that encode microbial surface components recognizing adhesive matrix molecules. All isolates were positive for icaAD, icaBC, fnbA, fib, sasC and cna, and negative for clfA, bbp and bap. The diversity of clfB, fnbB, sasG, eno and ebpS genes was found to exist. It was remarkable that only biofilm‐formation strains were positive for clfB. Furthermore, the effects of the NaCl concentration; glucose, sucrose, lactose and mannitol concentration; pH value; and growth media (Luria broth, basic medium, tryptic soy broth, 7.5% sodium chloride broth and common broth) on S. aureus biofilm formation were compared. Basic medium was proved in favor of biofilm formation. 7.5% sodium chloride broth went against biofilm formation. In detail, different saccharides could effectively promote biofilm formation. Contrarily, the biofilm formation was continuously decreased with increasing NaCl concentration. The effect of pH values on biofilm formation was significant. The rise or drop in pH value (≥12 or ≤4) was directly involved in the decrease in the biofilm formation. Taken together, the biofilm formation of S. aureus influenced by multiple factors and different adhesin genes, which may contribute to biofilm phenotype, possesses diversity.PRACTICAL APPLICATIONSStaphylococcus aureus biofilm formation presents a serious clinical problem. Environmental factor and cultivation conditions contribute a lot to the biofilm development in many S. aureus clinical isolates. Comparing the effect of different cultivation conditions on S. aureus biofilm formation and detecting the presence of different adhesive matrix molecule genes might help clarify the diversity of biofilm formation in foodborne outbreak strains. Applying appropriate sodium chloride solution and proper pH values could be an available way for controlling biofilm formation in S. aureus.
CHANGES IN LISTERIA MONOCYTOGENES INDUCED BY ORIGANUM VULGARE L. and ROSMARINUS OFFICINALIS L. ESSENTIAL OILS ALONE AND COMBINED AT SUBINHIBITORY AMOUNTS
Authors: DE AZERÊDO GEÍZA ALVES , DE FIGUEIREDO REGINA CELIA BRESSAN QUEIROZ , DE SOUZA EVANDRO LEITE , STAMFORD TÂNIA LÚCIA MONTENEGRO 
Published: April 2012

ABSTRACTThis study aimed to evaluate the antibacterial activities of the essential oils from Origanum vulgare L. and Rosmarinus officinalis L., both singly and in combination at subinhibitory concentrations against Listeria monocytogenes, and to investigate the possible mechanisms underlying these activities. Used singly or in a mixture, the essential oils led to a significant decrease in cell viability during 24 h of exposure. Decreased glucose consumption and the loss of cellular material by L. monocytogenes were found immediately after addition of the essential oils alone and in combination, and continued for up to 360 min. Analysis of cells exposed to the essential oils using electron microscopy revealed drastic ultrastructural changes occurring over time, including the changes in cell wall structure, rupture of plasma membranes, shrinking of the cytoplasmic content and leakage of intracellular material. O. vulgare L. and R. officinalis L. essential oils combined at subinhibitory concentrations could be rationally applied to inhibit the growth of L. monocytogenes in food products.PRACTICAL APPLICATIONSL. monocytogenes is a foodborne pathogen of particular concern in ready‐to‐eat products, such as minimally processed vegetables, because of its ability to survive and grow at refrigeration temperatures. The special risk posed by L. monocytogenes for the consumption of minimally processed vegetables has led to investigations on the development of novel technologies to control this contamination. In this context, plant essential oils have received particular interest for their potential to control L. monocytogenes in these foods. The findings of our study clearly indicate that the essential oil of oregano and rosemary, singly or in combination at subinhibitory concentrations, caused critical damages in L. monocytogenes cells. These essential oils combined at subinhibitory concentrations could be rationally applied to control the survival of L. monocytogenes in foods.
EFFECT OF STORAGE AT 4 AND 10C ON THE GROWTH OF LISTERIA MONOCYTOGENES IN AND ON QUESO FRESCO*
Authors: LEGGETT L.N. , TOMASULA P.M. , VAN HEKKEN D.L. , PORTO‐FETT A.C.S. , SHOYER B. , RENYE J.A. , LUCHANSKY J.B. , FARKYE N. 
Published: April 2012

ABSTRACTA five‐strain rifampicin‐resistant Listeria monocytogenes cocktail (ca. 3.0 log10 cfu/g) was introduced as a postpasteurization contaminant in queso fresco (QF) that was manufactured using a commercial procedure. L. monocytogenes was either inoculated into (IN) the curds before forming the cheese block or onto (ON) slices (52–66 g), individually vacuum‐packed and stored at 4 and 10C. Growth was monitored for up to 35 days. Gompertz analyses showed small differences in lag time because of temperature, but growth rate and generation time were faster at 10C than at 4C. After 20 days for both the IN and the ON treatments, the maximum population density was 7.80 ± 0.17, regardless of the storage temperature. These results indicate that QF manufacture must be conducted using Good Manufacturing Practices and under hygienic conditions, and that the use of antimicrobials and/or postprocessing interventions is necessary to prevent the presence and growth of L. monocytogenes.PRACTICAL APPLICATIONSQueso fresco (QF) is a popular, rennet‐set, Hispanic‐style fresh cheese made from pasteurized milk known for its crumbly texture and nonmelting properties. However, its high moisture content, near neutral pH and moderate salt content provide the ideal conditions for growth of various spoilage and pathogenic bacteria, notably Listeria monocytogenes. Past studies have examined the growth of L. monocytogenes on QF but were typically conducted using retail cheese of unknown manufacturing, handling and storage procedures. In this study, the growth of L. monocytogenes was monitored in and on QF made according to a commercial procedure used in the U.S.A. These data and a primary model that was created from the data can be used as the basis for food safety assessments and process and formulation improvements, or to monitor the effectiveness of antimicrobials and process interventions in preventing the presence and growth of L. monocytogenes associated with QF.
USE OF GRAM‐NEGATIVE MEDIUM AND IMMUNOMAGNETIC SEPARATIVE METHOD FOLLOWED BY MULTIPLEX POLYMERASE CHAIN REACTION FOR THE DETECTION OF ENTEROHEMORRHAGIC ESCHERICHIA COLI AND SALMONELLA SPP. WITH GREAT CELL COUNT DIFFERENCE IN FOOD SAMPLES
Authors: TSAI CHENG‐CHIH , HSIH HSIEN‐YEE , TSAI CHENG‐HSIEN , TSEN HAU‐YANG 
Published: April 2012

ABSTRACTContamination of different bacterial species with great cell count difference in food samples may affect the efficacy of multiplex polymerase chain reaction (mPCR) detection of these organisms. The purpose of this study was to improve the efficiency of mPCR detection of two pathogenic bacterial species, i.e., Salmonella and Shiga‐like toxin I Escherichia coli (SLTI EC), with great cell count difference in food samples. Without the enrichment step, when the cell count ratio of these two species was higher than 103, the bacteria presenting the lower number might go undetectable. For beef and milk samples with great ratios of SLTI EC/Salmonella, such as 103–105/100–101, if gram‐negative (GN) broth was used for the pre‐enrichment step, both organisms were detectable by mPCR. However, for samples with great ratios of Salmonella/SLTI EC, after enrichment with GN broth, an immunomagnetic separation (IMS) step using equimolar mixture of anti‐Salmonella and anti‐enterohemorrhagic Escherichia coli immunobeads had to be performed prior to mPCR to allow obvious results for both organisms. Therefore, although GN is a selective medium for both Salmonella and E. coli, depending on the ratios of the two target organisms in food samples, an IMS step may be required to allow the simultaneous PCR detection of both organisms.PRACTICAL APPLICATIONSAlthough multiplex polymerase chain reaction (mPCR) method allows the simultaneous detection of different target organisms, if the ratios of the cell counts for these target organisms were great, the PCR results for target organisms with lower counts will be obscured. Using gram‐negative broth for pre‐enrichment followed by mPCR or pre‐enrichment and immunomagnetic separation (IMS) followed by mPCR, we were able to simultaneously detect enterohemorrhagic Escherichia coli and Salmonella in food samples even the cell count ratios of these two organisms were great. Similar approach could be used for the simultaneous detection of two or more bacterial species in different samples.
Endocannabinoids modulate non‐adrenergic, non‐cholinergic inhibitory neurotransmission in strips from the mouse gastric fundus
Authors: Garella R. , Baccari M. C. 
Published: April 2012

AbstractAimTo investigate the effects of endocannabinoids on non‐adrenergic, non‐cholinergic (NANC) relaxant responses in gastric strips from mice.MethodsGastric longitudinal strips from the fundus region were mounted in organ baths for isometric recording.ResultsIn carbachol‐precontracted strips, electrical field stimulation (EFS) elicited tetrodotoxin (TTX)‐sensitive fast nitrergic relaxant responses that were followed, at the highest stimulation frequency, by sustained relaxations. The latter were abolished by α‐chymotrypsin. Anandamide caused a TTX‐sensitive relaxation that was abolished by α‐chymotrypsin but unaffected by the nitric oxide (NO) synthesis inhibitor, Nω‐nitro‐L‐arginine (L‐NNA). Anandamide reduced the amplitude of EFS‐induced fast relaxations, whereas increased that of sustained ones. Relaxation to the nicotinic receptor agonist dimethylphenyl piperazinium iodide (DMPP) was decreased in amplitude by either anandamide or L‐NNA, whereas, surprisingly, it was increased by α‐chymotrypsin and abolished by L‐NNA plus α‐chymotrypsin. Relaxation to vasoactive intestinal polypeptide (VIP) was not influenced by anandamide or L‐NNA and was abolished by α‐chymotrypsin. Following VIP desensitization, fast relaxant responses to EFS were reduced and the sustained ones abolished. The CB1 receptor antagonist AM251 increased, only at the highest stimulation frequency, the amplitude of the EFS‐induced fast relaxation and reduced the sustained one. AM251 increased the response to DMPP and abolished that to anandamide. The CB2 receptor antagonist AM630 had no effects.ConclusionThese results indicate that endocannabinoids modulate, via prejunctional CB1 receptors, the NANC peptidergic neurotransmission that, in turn, affects the nitrergic one.