bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accurate Classification of Non–Small Cell Lung Carcinoma Using a Novel MicroRNA-Based Approach
Authors: Bishop Justin A. , Benjamin Hila , Cholakh Hila , Chajut Ayelet , Clark Douglas P. , Westra William H. 
Published: January 2010

Abstract
Purpose: Advances in targeted lung cancer therapy now demand accurate classification of non–small cell lung cancer (NSCLC). MicroRNAs (miRNA) are recently discovered short, noncoding genes that play essential roles in tissue differentiation during normal development and tumorigenesis. For example, hsa-miR-205 is a miRNA that is highly expressed in lung squamous cell carcinomas (SqCC) but not in lung adenocarcinomas. The differential expression of miRNAs could be exploited to distinguish these tumor types.
Experimental Design: One hundred and two resected NSCLCs were classified as SqCC or adenocarcinoma based on their histologic features and immunohistochemical profiles. Corresponding preoperative biopsies/aspirates that had been originally diagnosed as poorly differentiated NSCLCs were available for 21 cases. A quantitative reverse transcription-PCR diagnostic assay that measures the expression level of hsa-miR-205 was used to classify the carcinomas as SqCC or adenocarcinoma based solely on expression levels. The two sets of diagnoses were compared.
Results: Using standard pathologic methods of classification (i.e., microscopy and immunohistochemistry), 52 resected lung carcinomas were classified as SqCCs and 50 as adenocarcinomas. There was 100% concordance between the diagnoses established by conventional and miRNA-based methods. MiRNA profiling also correctly classified 20 of the 21 preoperative biopsy specimens.
Conclusions: MiRNA profiling is a highly reliable strategy for classifying NSCLCs. Indeed, classification is consistently accurate even in small biopsies/aspirates of poorly differentiated tumors. Confirmation of its reliability across the full range of tumor grades and specimen types represents an important step toward broad application. Clin Cancer Res; 16(2); 610–9
The TRC8 Ubiquitin Ligase Is Sterol Regulated and Interacts with Lipid and Protein Biosynthetic Pathways
Authors: Lee Jason P. , Brauweiler Anne , Rudolph Michael , Hooper Joan E. , Drabkin Harry A. , Gemmill Robert M. 
Published: January 2010

Abstract
TRC8/RNF139 encodes an endoplasmic reticulum–resident E3 ubiquitin ligase that inhibits growth in a RING- and ubiquitylation-dependent manner. TRC8 also contains a predicted sterol-sensing domain. Here, we report that TRC8 protein levels are sterol responsive and that it binds and stimulates ubiquitylation of the endoplasmic reticulum anchor protein INSIG. Induction of TRC8 destabilized the precursor forms of the transcription factors SREBP-1 and SREBP-2. Loss of SREBP precursors was proteasome dependent, required a functional RING domain, occurred without generating processed nuclear forms, and suppressed SREBP target genes. TRC8 knockdown had opposite effects in sterol-deprived cells. In Drosophila, growth inhibition by DTrc8 was genetically suppressed by loss of specific Mprlp, Padlp N-terminal domain–containing proteins found in the COP9 signalosome and eIF3. DTrc8 genetically and physically interacted with two eIF3 subunits: eIF3f and eIF3h. Coimmunoprecipitation experiments confirmed these interactions in mammalian cells, and TRC8 overexpression suppressed polysome profiles. Moreover, high–molecular weight ubiquitylated proteins were observed in eIF3 immunoprecipitations from TRC8-overexpressing cells. Thus, TRC8 function may provide a regulatory link between the lipid and protein biosynthetic pathways. Mol Cancer Res; 8(1); 93–106
Cancer Chemopreventive Activity and Metabolism of Isoliquiritigenin, a Compound Found in Licorice
Authors: Cuendet Muriel , Guo Jian , Luo Yan , Chen Shaonong , Oteham Carol P. , Moon Richard C. , van Breemen Richard B. , Marler Laura E. , Pezzuto John M. 
Published: January 2010

Abstract
Isoliquiritigenin (2′,4′,4-trihydroxychalcone; ILG), a chalcone found in licorice root and many other plants, has shown potential chemopreventive activity through induction of phase II enzymes such as quinone reductase-1 in murine hepatoma cells. In this study, the in vivo metabolism of ILG was investigated in rats. In addition, ILG glucuronides and ILG-glutathione adducts were observed in human hepatocytes and in livers from rats treated with ILG. ILG glucuronides were detected in both plasma and rat liver tissues. In addition, in a full-term cancer chemoprevention study conducted with 7,12-dimethylbenz(a)anthracene–treated female Sprague-Dawley rats, dietary administration of ILG slightly increased tumor latency but had a negative effect on the incidence of mammary tumors starting at ∼65 days after 7,12-dimethylbenz(a)anthracene administration. Further, no significant induction of phase II enzymes was found in mammary glands, which is consistent with the low level of ILG observed in these tissues. However, ILG significantly induced quinone reductase-1 activity in the colon, and glutathione as well as glutathione S-transferase in the liver. Analysis of mRNA expression in tissues of rats treated with ILG supported these findings. These results suggest that ILG should be tested for chemopreventive efficacy in nonmammary models of cancer. Cancer Prev Res; 3(2); 221–32
Interactions of natural and sexual selection: damselfish prioritize brood defense with male–male competition or courtship
Authors: Leese Joseph , Snekser Jennifer , Itzkowitz Murray 
Published: January 2010

AbstractNatural and sexual selection often act in opposing directions, forcing individuals to prioritize activities necessary for survival with those required for reproduction. We examined the interaction of natural and sexual selection pressures on the behaviour of beaugregory damselfish, Stegastes leucostictus, by presenting territorial males with an egg predator and either a male or female conspecific, requiring males to prioritize brood defense with either courtship or intra-sexual competition. By measuring the time spent near stimuli, we examined the influence of several factors, including stimuli type, variation in predator number, and the presence of eggs in a nest site. Generally, males spent more time with conspecific stimuli than egg predators, suggesting that the sexual selection pressures of deterring rivals and attracting mates outweigh the natural selection pressure to engage in brood defense. This decision was affected by the sex of the conspecific presented, the presence of eggs in a male's territory, and a number of interactions of these factors, indicating that male damselfish consider multiple factors before investing in certain types and intensities of behaviours. Furthermore, observations of behaviours associated with courtship and intrasexual aggression show that a natural selection pressure impacts intra- and inter-sexually selected behaviours differently in this system.
Seeking Justice among the 'Outsiders': Christian Recourse to Non-Ecclesiastical Judicial Systems under Early Islam
Authors: Simonsohn Uriel 
Published: January 2010

AbstractThe phenomenon of Christian recourse to non-ecclesiastical judicial systems during the first few centuries following the Muslim conquest is at the core of this paper. This phenomenon not only alludes to a reality of legal diversity but also to the social heterogeneity that has characterized Near Eastern societies long before the Arab takeover. Through the adoption of the legal-anthropological paradigm of legal pluralism and the examination of West Syrian legal sources, the paper seeks to identify the social agenda of West Syrian ecclesiastical leaders. Much of the discussion revolves around the term 'outsiders', barrāyē, and attempts to delineate the term's various meanings within the legal discourse.
Tradition and the Formation of the 'Nestorian' Identity in Sixth- to Seventh-Century Iraq
Authors: Reinink Gerrit 
Published: January 2010

AbstractReligious identities in ancient Near Eastern Christianity were mainly and primarily defined along the lines of Christological positions held by the different Christian communities. This article discusses the origin, development, and propagation of the East Syrian 'Nestorian' Christology of the two natures and two hypostaseis in Christ. It is argued that the process of the formation of the East Syrian Christological identity took a relative long time due to the complex and pluriform cultural tradition in East Syrian Christianity by the end of the sixth century and the radically changing historical, political, and social conditions in late sixth- and early seventh-century Iraq.
Dominance and Diversity: Kingship, Ethnicity, and Christianity in Orthodox Ethiopia
Authors: Kaplan Steven 
Published: January 2010

AbstractThe purpose of this article is to survey the history of the Ethiopian Orthodox Church with an emphasis on several features which are of significance for comparison to Syriac Orthodox Christianity. Although it focuses primarily on the period from 1270 during which 'Ethiopian' was a national rather than ethnic identity, it shares several themes with other papers in this volume. After considering the manner in which Christianity reached Ethiopia and in particular the central role played by the royal court in the acceptance and consolidation of the Church, attention is given to the claims of successive Ethiopian rulers and ethnic groups to be 'Israelites', that is, descendants of biblical figures most notably King Solomon and the Queen of Sheba. The paper next considers the manner in which monastic movements, which emerged in the fourteenth and fifteenth centuries, were associated with ethnically based resistance to the expansion of the Christian kingdom. Other themes include the development of a tradition of biblical interpretation and Christological controversies. The paper concludes with a discussion of ongoing research concerning the Ethiopian diaspora which has developed in the period since the Marxist revolution of 1974.
Family Matters: Community, Ethnicity, and Multiculturalism
Authors: Hofstee Willem 
Published: January 2010

AbstractConcepts which play an important role in the theoretical framework of community studies, such as community, identity, nation and nation state, ethnicity, and multiculturalism, are being discussed and questioned. It is argued that the idea of family resemblances should form the basis of any comparison, and that the circumstances in which these concepts are being expressed should be studied first, instead of using a checklist in order to establish them.
Syriac Historiography and Identity Formation
Authors: Debié Muriel 
Published: January 2010

AbstractHistoriographical texts are here read as literary compositions of their time, providing us with various elements of the process of identity construction or reconstruction. The first West Syrian historical texts were produced in the sixth century, when the history of what would become the Syrian Orthodox Church began. An examination of contemporary sources and myths of origins shows that the ethnic origins of the Abgarid dynasty played no part in Syrian 'ethnogenesis', but that there existed a notion of Syro-Mesopotamian origins, closely related to a supposed homeland, that of Aram. An acknowledged common ancestry going back to the Chaldean and Assyrian Empires relies on a common language more than a common homeland or sovereign. Whereas the Assyrians came to personify the ever-hostile Persian neighbour, a sort of stereotypical enemy, the Hellenistic kings were perceived as having effected a synthesis of the double Syro-Mesopotamian and Greek culture. The Seleucid era, as adopted by the Edessans, thus remained in use regardless of the prevailing political powers and is an assertion of independence and a strong local identity marker, being a rejection of the local Antiochene as well as the imperial Byzantine eras. The Syrian Orthodox also developed an innovative method of writing the history of their separated Church, producing a new genre consisting of lengthy chronicles written in several parts or columns, in which political and ecclesiastical history were kept separate. This Syrian Orthodox method of writing history is the only truly distinctive Syrian Orthodox literary genre.
Iconography and Identity: Syrian Elements in the Art of Crusader Cyprus
Authors: Carr Annemarie Weyl 
Published: January 2010

AbstractThe murals of triumphal arch in the Church of the Panagia Phorbiotissa, Cyprus, painted in the late thirteenth century when Cyprus was a Crusader state, adopt an iconography paralleled not in Byzantium but in the Miaphysite churches of the Syrian and Egyptian mainland, and best analyzed in relation to Miaphysite liturgical exegesis. As such, they suggest three revisions to current ways of thinking about the roles of Cyprus and the mainland in shaping the art of the Crusader era: 1) rather than for a 'maniera cypria' or a 'maniera tripolitana', we must look for an intricate, two-way reciprocity; 2) it is a reciprocity not simply between Cyprus and the mainland Crusader states, but between Cyprus and the far larger terrain of Syrian and Egyptian eastern Christendom; and 3) it engages not only style but also iconography and content.
Art and Identity in an Amulet Roll from Fourteenth-Century Trebizond
Authors: Peers Glenn 
Published: January 2010

AbstractThis article examines a unique survival from the Middle Ages: an amulet roll, now divided between libraries in New York City and Chicago, which now measures approximately 5 m in width and 8–9 cm in width, which has Greek texts on the obverse and Arabic on the reverse, and a series of very fine illustrations on the Greek side. Analysis of the roll reveals that it originated in Trebizond in the second half of the fourteenth century, and the roll is therefore considered within the cultural and political context of that small but active Greek kingdom. The article pays particular attention to the text and representation of a rare figure, Evgenios of Trebizond, who is included among a series of saints and prophets in order to enact that saint's protection of the (evidently elite) patron of the roll. And through the series of texts and images about the letter and self-portrait of Christ, the Mandylion, the roll also stated the sacred destiny of Trebizond. The roll generated identity through its Greek Christian texts and images, and made clear the special role God had chosen for Trebizond.
The Development of a Chalcedonian Identity in Byzantium (451–553)
Authors: Price Richard 
Published: January 2010

AbstractThe Byzantine Church adopted a Chalcedonian identity only slowly. At first the majority even of Chalcedonians played down the significance of the council, claiming that it did little more than repeat the teaching of the Nicene Creed. Down to 518 committed Chalcedonians, strongly upholding the teaching of the council, were vocal, but few. It is with Justin I (518–527) and his nephew Justinian I (527–565) that State and Church came to insist on the council. Justinian's commitment to it has sometimes been doubted because of his repeated attempts to win back the non-Chalcedonians (Miaphysites) to the imperial Church by inviting them back without requiring subscription to the Chalcedonian Definition. He was motivated by a desire that even the Miaphysites would look to him as their patron, as required for the maintenance of the unity of the empire. But his theological writings make it clear that he was convinced of the truth of the teaching of Chalcedon. The age of Justinian thus saw the attainment of a truly Chalcedonian identity in the imperial Church. This was a matter of official doctrine. In the sphere of popular piety Chalcedon had less impact. The affirmation of Chalcedon shaped Byzantine communal identity less than the rejection of Chalcedon shaped that of Miaphysite Syria and Egypt.
Jejunal Feeding Tubes Can Be Efficiently and Independently Placed by Intensive Care Unit Teams
Authors: Welpe Pascal , Frutiger Adrian , Vanek Patrik , Kleger Gian‐Reto 
Published: January 2010

Background:Nutrition support is an important therapeutic measure in critically ill patients. Several studies have shown that the enteral route is preferable to the parenteral route. Insertion of a feeding tube beyond the ligament of Treitz combined with continuous gastric drainage will reduce regurgitation and probably also the rate of nosocomial pneumonia. This study was conducted to assess the safety, success rate, and time required to establish jejunal nutrition by the fluoroscopy‐guided technique in intensive care unit (ICU) patients.Methods:This was a prospective observational study in the ICUs of a 300‐bed and a 600‐bed community hospital. Indications were large gastric residuals during attempted gastric feeding, severe acute pancreatitis, or recurrent aspiration. Feeding tubes were introduced by the ICU staff at bedside under fluoroscopic guidance (a senior ICU physician and a resident or a registered ICU nurse). The correct jejunal position was documented by the application of a radiopaque contrast medium through the tube. After confirmation of the correct position, jejunal tube feeding was immediately started.Results:The insertion procedure in 38 patients lasted a median of 17 minutes. The median time from decision to place the tube until start of enteral feeding was 141 minutes. The success rate was 84.2%. No adverse events were observed.Conclusions:Fluoroscopic placement of a jejunal feeding tube at the bedside is fast, is safe, and has a high success rate when performed by well‐trained ICU staff. Using this method makes the ICU team more self‐sufficient when critically ill patients require enteral nutrition and no gastroenterologist is available.
CD137 agonist antibody prevents cancer recurrence: contribution of CD137 on both hematopoietic and nonhematopoietic cells
Authors: Narazaki Hidehiko , Zhu Yuwen , Luo Liqun , Zhu Gefeng , Chen Lieping 
Published: January 2010

AbstractAntigen-specific memory T cells (Tms) are essential in the immune surveillance of residual and metastatic tumors. Activation of Tms requires designing vaccines based on tumor rejection antigens, which are often not available to cancer patients. Therefore, it is desirable to have a general applicable approach to activate Tms without extensive knowledge of tumor antigens. Here, we report that activation of antigen-specific Tms could be achieved by the administration of agonistic anti-CD137 monoclonal antibody without additional tumor vaccination, leading to the prevention of recurrence and metastases after surgical resection of primary tumors in mouse models. By reconstitution with CD137-deficient Tms, we demonstrate that expression of CD137 on antigen-specific Tms is only partially required for the effect of anti-CD137 antibody. Other host cells, including those from hematopoietic and nonhematopoietic origins, are also important because ablation of CD137 from these cells partially but significantly eliminates antitumor effect of anti-CD137 antibody. Our findings implicate a potential new approach to prevent recurrence and metastases in cancer patients.
H4R3 methylation facilitates β-globin transcription by regulating histone acetyltransferase binding and H3 acetylation
Authors: Li Xingguo , Hu Xin , Patel Bhavita , Zhou Zhuo , Liang Shermi , Ybarra River , Qiu Yi , Felsenfeld Gary , Bungert Jörg , Huang Suming 
Published: January 2010

AbstractHistone modifications play an important role in the process of transcription. However, in contrast to lysine methylation, the role of arginine methylation in chromatin structure and transcription has been underexplored. The globin genes are regulated by a highly organized chromatin structure that juxtaposes the locus control region (LCR) with downstream globin genes. We report here that the targeted recruitment of asymmetric dimethyl H4R3 catalyzed by PRMT1 (protein arginine methyltransferase 1) facilitates histone H3 acetylation on Lys9/Lys14. Dimethyl H4R3 provides a binding surface for P300/CBP-associated factor (PCAF) and directly enhances histone H3 acetylation in vitro. We show that these active modifications are essential for efficient interactions between the LCR and the βmaj-promoter as well as transcription of the β-globin gene. Furthermore, knockdown (KD) of PRMT1 by RNA interference in erythroid progenitor cells prevents histone acetylation, enhancer and promoter interaction, and recruitment of transcription complexes to the active β-globin promoter. Reintroducing rat PRMT1 into the PRMT1 KD MEL cells rescues PRMT1 binding, β-globin transcription, and erythroid differentiation. Taken together, our data suggest that PRMT1-mediated dimethyl H4R3 facilitates histone acetylation and enhancer/promoter communications, which lead to the efficient recruitment of transcription preinitiation complexes to active promoters.
Donor and recipient chemokine receptor CCR5 genotype is associated with survival after bone marrow transplantation
Authors: McDermott David H. , Conway Susan E. , Wang Tao , Ricklefs Stacy M. , Agovi Manza A. , Porcella Stephen F. , Tran Huong Thi Bich , Milford Edgar , Spellman Stephen , Abdi Reza 
Published: January 2010

AbstractDespite continual improvement, morbidity and mortality after hematopoietic stem cell transplantation (HSCT) remain high. The importance of chemokines in HSCT lies in their regulation of immune responses that determine transplantation outcomes. We investigated the role of recipient and donor chemokine system gene polymorphisms by using a candidate gene approach on the incidence of graft-versus-host disease and posttransplantation outcomes in 1370 extensively human leukocyte antigen–matched, unrelated donor-recipient pairs by using multivariate Cox regression models. Our analysis identified that recipients homozygous for a common CCR5 haplotype (H1/H1) had better disease-free survival (DFS; P = .005) and overall survival (P = .021). When the same genotype of both the donor and recipient were considered in the models, a highly significant association with DFS and overall survival was noted (P < .001 and P = .007, respectively) with absolute differences in survival of up to 20% seen between the groups at 3 years after transplantation (50% DFS for pairs with recipient CCR5 H1/H1 vs 30% for pairs with donor CCR5 H1/H1). This finding suggests that donor and/or recipient CCR5 genotypes may be associated with HSCT outcome and suggests new diagnostic and therapeutic strategies for optimizing therapy.
Skin-draining lymph nodes contain dermis-derived CD103− dendritic cells that constitutively produce retinoic acid and induce Foxp3+ regulatory T cells
Authors: Guilliams Martin , Crozat Karine , Henri Sandrine , Tamoutounour Samira , Grenot Pierre , Devilard Elisabeth , de Bovis Béatrice , Alexopoulou Lena , Dalod Marc , Malissen Bernard 
Published: January 2010

Abstract
Small intestinal CD103+ dendritic cells (DCs) have the selective ability to promote de novo generation of regulatory T cells via the production of retinoic acid (RA). Considering that aldehyde dehydrogenase (ALDH) activity controls the production of RA, we used a flow cytometry–based assay to measure ALDH activity at the single-cell level and to perform a comprehensive analysis of the RA-producing DC populations present in lymphoid and nonlymphoid mouse tissues. RA-producing DCs were primarily of the tissue-derived, migratory DC subtype and can be readily found in the skin and in the lungs as well as in their corresponding draining lymph nodes. The RA-producing skin-derived DCs were capable of triggering the generation of regulatory T cells, a finding demonstrating that the presence of RA-producing, tolerogenic DCs is not restricted to the intestinal tract as previously thought. Unexpectedly, the production of RA by skin DCs was restricted to CD103− DCs, indicating that CD103 expression does not constitute a “universal” marker for RA-producing mouse DCs. Finally, Toll-like receptor (TLR) triggering or the presence of a commensal microflora was not essential for the induction of ALDH activity in the discrete ALDH+ DC subsets that characterize tissues constituting environmental interfaces.