bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genome-wide associations with longevity and reproductive traits in U.S. rangeland ewes
Authors: Smitchger Jamin A. , Taylor J. Bret , Mousel Michelle R. , Schaub Daniel , Thorne Jacob W. , Becker Gabrielle M. , Murdoch Brenda M. 
Published: May 2024

Introduction: Improving ewe longevity is an important breeding and management goal, as death loss and early culling of mature ewes are economic burdens in the sheep industry. Ewe longevity can be improved by selecting for positive reproductive outcomes. However, the breeding approaches for accomplishing this come with the challenge of recording a lifetime trait. Characterizing genetic factors underpinning ewe longevity and related traits could result in the development of genomic selection strategies to improve the stayability of sheep through early, informed selection of replacement ewes.Methods: Towards this aim, a genome-wide association study (GWAS) was performed to identify genetic markers associated with ewe longevity, reproductive, and production traits. Traits evaluated included longevity (i.e., length of time in the flock), parity and the lifetime number of lambs born, lambs born alive, lambs weaned, and weight of lambs weaned. Ewe records from previous studies were used. Specifically, Rambouillet (n = 480), Polypay (n = 404), Suffolk (n = 182), and Columbia (n = 64) breed ewes (N = 1,130) were analyzed against 503,617 SNPs in across-breed and within-breed GWAS conducted with the Bayesian-information and Linkage-disequilibrium Iteratively Nested Keyway (BLINK) model in R.Results: The across-breed GWAS identified 25 significant SNPs and the within-breed GWAS for Rambouillet, Polypay, and Suffolk ewes identified an additional 19 significant SNPs. The most significant markers were rs411309094 (13:22,467,143) associated with longevity in across-breed GWAS (p-value = 8.3E-13) and rs429525276 (2:148,398,336) associated with both longevity (p-value = 6.4E-15) and parity (p-value = 4.8E-15) in Rambouillet GWAS. Significant SNPs were identified within or in proximity (±50 kb) of genes with known or proposed roles in reproduction, dentition, and the immune system. These genes include ALPL, ANOS1, ARHGEF26, ASIC2, ASTN2, ATP8A2, CAMK2D, CEP89, DISC1, ITGB6, KCNH8, MBNL3, MINDY4, MTSS1, PLEKHA7, PRIM2, RNF43, ROBO2, SLCO1A2, TMEM266, TNFRSF21, and ZNF804B.Discussion: This study proposes multiple SNPs as candidates for use in selection indices and suggests genes for further research towards improving understanding of the genetic factors contributing to longevity, reproductive, and production traits of ewes.
Exploring effects of brief daily exposure to unfamiliar accent on listening performance and cognitive load
Authors: McLaughlin Drew J. , Baese-Berk Melissa M. , Van Engen Kristin J. 
Published: May 2024

IntroductionListeners rapidly “tune” to unfamiliar accented speech, and some evidence also suggests that they may improve over multiple days of exposure. The present study aimed to measure accommodation of unfamiliar second language- (L2-) accented speech over a consecutive 5-day period using both a measure of listening performance (speech recognition accuracy) and a measure of cognitive load (a dual-task paradigm).MethodsAll subjects completed a dual-task paradigm with L1 and L2 accent on Days 1 and 5, and were given brief exposure to either L1 (control group) or unfamiliar L2 (training groups) accent on Days 2–4. One training group was exposed to the L2 accent via a standard speech transcription task while the other was exposed to the L2 accent via a transcription task that included implicit feedback (i.e., showing the correct answer after each trial).ResultsAlthough overall improvement in listening performance and reduction in cognitive load were observed from Days 1 to 5, our results indicated neither a larger benefit for the L2 accent training groups compared to the control group nor a difference based on the implicit feedback manipulation.DiscussionWe conclude that the L2 accent trainings implemented in the present study did not successfully promote long-term learning benefits of a statistically meaningful magnitude, presenting our findings as a methodologically informative starting point for future research on this topic.
Enhancing stroke risk prediction in patients with transient ischemic attack: insights from a prospective cohort study implementing fast-track care
Authors: Barone Valentina , Foschi Matteo , Pavolucci Lucia , Rondelli Francesca , Rinaldi Rita , Nicodemo Marianna , D’Angelo Roberto , Favaretto Elisabetta , Brusi Carlotta , Cosmi Benilde , Degli Esposti Daniela , D’Addato Sergio , Bacchelli Stefano , Giostra Fabrizio , Pomata Daniela Paola , Spinardi Luca , Faccioli Luca , Faggioli Gianluca , Donti Andrea , Borghi Claudio , Cortelli Pietro , Guarino Maria ,   Bologna TIA Study Group
Published: May 2024

Background and aimsFast-track care have been proved to reduce the short-term risk of stroke after transient ischemic attack (TIA). We aimed to investigate stroke risk and to characterize short- and long-term stroke predictors in a large cohort of TIA patients undergoing fast-track management.MethodsProspective study, enrolling consecutive TIA patients admitted to a Northern Italy emergency department from August 2010 to December 2017. All patients underwent fast-track care within 24 h of admission. The primary outcome was defined as the first stroke recurrence at 90 days, 12 and 60 months after TIA. Stroke incidence with 95% confidence interval (CI) at each timepoint was calculated using Poisson regression. Predictors of stroke recurrence were evaluated with Cox regression analysis. The number needed to treat (NNT) of fast-track care in preventing 90-day stroke recurrence in respect to the estimates based on baseline ABCD2 score was also calculated.ResultsWe enrolled 1,035 patients (54.2% males). Stroke incidence was low throughout the follow-up with rates of 2.2% [95% CI 1.4–3.3%] at 90 days, 2.9% [95% CI 1.9–4.2%] at 12 months and 7.1% [95% CI 5.4–9.0%] at 60 months. Multiple TIA, speech disturbances and presence of ischemic lesion at neuroimaging predicted stroke recurrence at each timepoint. Male sex and increasing age predicted 90-day and 60-month stroke risk, respectively. Hypertension was associated with higher 12-month and 60-month stroke risk. No specific TIA etiology predicted higher stroke risk throughout the follow-up. The NNT for fast-track care in preventing 90-day stroke was 14.5 [95% CI 11.3–20.4] in the overall cohort and 6.8 [95% CI 4.6–13.5] in patients with baseline ABCD2 of 6 to 7.ConclusionOur findings support the effectiveness of fast-track care in preventing both short- and long-term stroke recurrence after TIA. Particular effort should be made to identify and monitor patients with baseline predictors of higher stroke risk, which may vary according to follow-up duration.
Causal association of sarcopenia with hepatocellular carcinoma risk in European population: a Mendelian randomization study
Authors: Cao Jiali , Huang Yumei , Zhu Mengpei , Wang Ziwen , Jin Ze , Xiong Zhifan 
Published: May 2024

BackgroundThe causal association of sarcopenia with the incidence risk of hepatocellular carcinoma (HCC) in the European population, and the potential mediating role of C-reactive protein (CRP), remains unclear. This study employed a bidirectional two-sample, two-step Mendelian randomization (MR) analysis to investigate the causality and identify the mediator.MethodsSummary statistics for HCC, CRP, and sarcopenia-related traits, including appendicular lean mass (ALM), hand grip strength (HGS), and walking pace (WP), were acquired from publicly available databases. We conducted bidirectional MR and Steiger tests of directionality to check the presence of reverse causality. Additionally, a two-step MR analysis was used to assess the mediating effect of CRP in the causality between sarcopenia and HCC. Tests for heterogeneity and horizontal pleiotropy were performed.ResultsAs ALM increases, the risk of HCC occurrence decreases [odds ratio (OR), 95% confidence interval (CI): 0.703, 0.524–0.943; P = 0.019]. And, genetically predicted low-HGS (OR, 95%CI: 2.287, 1.013–5.164; P = 0.047) was associated with an increased incidence risk of HCC, with no reverse causality. However, we found no evidence supporting a causality between WP and HCC. CRP was identified as the mediator of the causal effect of ALM and low-HGS on HCC, with corresponding mediating effects of 9.1% and 7.4%.ConclusionsThis MR study effectively demonstrates that lower ALM and low-HGS are linked to an elevated risk of HCC within the European population, and the causality was not bidirectional. Furthermore, CRP serves as a mediator in the associations. These findings may help mitigate HCC risk among individuals with sarcopenia.
Multislice helical computed tomography imaging diagnosis and surgical treatment of primary tracheal tumor in cardiothoracic surgery
Authors: Rui Jun , Lian Yu 
Published: May 2024

Objectiveit aimed to explore the value of multislice helical computed tomography (MSCT) in the diagnosis and surgical treatment of primary tracheal tumors.Methods64 patients with the primary tracheal tumor who were diagnosed in Wuxi Second People’s Hospital from March 2020 to March 2021 were selected as the research objects. MSCT imaging was performed on all patients, and suitable surgical methods. The pathological results were compared with original CT, CT virtual endoscopy (CTVE), and Comparisons were made using CT three-dimensional reconstruction images to evaluate the accuracy of MSCT diagnosis. Parameters such as postoperative complications and survival rates were observed to assess surgical effectiveness and safety.ResultsCompared with original CT images (70%, 72%, 70%), the diagnostic accuracy of VR images (80%, 80%, 80%), MPVR images (85%, 90%, 92%), and CTVE images (100%, 100%, 100%) was remarkably improved (P&lt;0.05). The three-year survival rate of patients with smooth muscle tumors, malignant tumors, salivary gland adenoma, papillary tumors, and inflammatory polyp was markedly lower than that of the one-year survival rate, with a significant difference (P&lt;0.05). The incidence of postoperative complications was 14.1%, with three cases resulting in complication-related deaths.Conclusionthe diagnostic accuracy of MSCT imaging of primary tracheal tumor was high. The diagnostic accuracy of CTVE was higher than that of VR and MPVR. Besides, surgical treatment of primary tracheal tumor had a substantial effect, with no serious postoperative complications.
Sophora flavescens-Astragalus mongholicus herb pair in the progression of hepatitis, cirrhosis, and hepatocellular carcinoma: a possible mechanisms and relevant therapeutic substances
Authors: Yang Xiao , Liang Chen , Shao Li , Cui Wenxuan , Ning Ruobing , Ke Fan , Wang Yue , Gao Peng , Yin Yidi , Li Qing 
Published: May 2024

BackgroundBoth Sophora flavescens (SF) and Astragalus mongholicus (AM) are known for their anti-inflammatory, antifibrotic, and anticancer activities. However, the efficacy, multi-target mechanisms, and therapeutic substances of SF-AM herb pair on the progression of hepatitis-cirrhosis-hepatocellular carcinoma hepatocellular carcinoma (HCC) remain unclear.PurposeTo investigate the efficacy, mechanisms, and potential therapeutic substances of SF-AM herb pair in the progression of hepatitis-cirrhosis-HCC.MethodsFirstly, diethylnitrosamine was used to establish the hepatitis-cirrhosis-HCC model. HE staining and non-targeted metabolomics were used to evaluate the efficacy of SF-AM herb pair. Subsequently, the absorbed components of SF-AM herb pair in the plasma of rats were determined through HPLC-Q-TOF-MS/MS analysis. Flow cytometry, Western blot, and qRT-PCR were then employed to assess CD4+ and CD8+ T lymphocytes, PI3K/Akt signaling pathway-related proteins, and their corresponding mRNAs. Simultaneously, the efficacy and mechanism of SF-AM herb pair on HCC were confirmed by in vitro experiments. Finally, Pearson correlation analysis was performed between pharmacodynamic indicators and in vivo components to identify the potential therapeutic substances of SF-AM herb pair.ResultsSF-AM herb pair can alleviate the pathological damage and reverse metabolic abnormalities in hepatitis, cirrhosis, and HCC rats, particularly during the hepatitis and cirrhosis stages. Pharmacological researches have demonstrated that SF-AM herb pair can increase the proportion of CD8+ T lymphocytes, inhibit the expression of PI3K, Akt, p-Akt, NF-κB p65, NF-κB pp65, and Bcl-2, as well as increase the expression of IκBα, Bax, and cleaved caspase-3. These findings suggest that SF-AM herb pair has the ability to enhance immunity, anti-inflammation and promote apoptosis. Cell experiments have shown that SF-AM herb pair can inhibit the proliferation of HepG2 cell and regulate the PI3K/Akt signaling pathway. Moreover, 23 absorbed prototypical components and 53 metabolites of SF-AM herb pair were identified at different stages of HCC rats. Pearson correlation analysis revealed that matrine, cytisine, wogonoside, and isoastragaloside are potential therapeutic substances in SF-AM herb pair for the prevention and treatment of hepatitis, cirrhosis, and HCC.ConclusionIn summary, this study revealed the efficacy, mechanisms, and potential therapeutic substances of SF-AM herb pair in the hepatitis-cirrhosis-HCC axis and provided a reference for its clinical application.
Therapeutic effects of vitamin D supplementation on COVID-19 aggravation: a systematic review and meta-analysis of randomized controlled trials
Authors: Yang Yiyuan , Sun Wanli , Yang Fan , Zhang Guoxia , Li Xinye , Sun Shipeng , Xing Yanwei 
Published: May 2024

BackgroundThe therapeutic effects of vitamin D supplementation on Coronavirus disease 2019 (COVID-19) aggravation remain controversial and inconclusive. To probe into this contentious issue, we performed the present meta-analysis of randomized controlled trials (RCTs).MethodsLiterature published up to June 2023 was retrieved from Cochrane Library, PubMed, Web of Science and Embase. RCTs assessing mortality, intensive care unit (ICU) admission, mechanical ventilation (MV), length of hospitalization (LOH), and inflammatory markers containing C-reactive protein (CRP), D-dimer, interleukin-6 (IL-6), lactate dehydrogenase (LDH) were included. 19 RCTs were involved in the analysis and were conducted subgroup analyses on the baseline COVID-19 severity and vitamin D administration.ResultsIn the severity subgroup, statistically significant effects in moderate to severe group were observed in ICU admission (OR 0.43, 95% CI 0.23, 0.80; p = 0.008), MV (OR 0.44, 95% CI 0.27, 0.72; p = 0.001) and LOH (SMD –0.49, 95% CI –0.92, −0.06; p = 0.027). In the administration subgroup, effects of ICU admission (OR 0.39, 95% CI 0.16, 0.97; p = 0.044), MV (OR 0.18, 95% CI 0.07, 0.46; p = 0.000) and LOH (SMD –0.50, 95% CI –0.96, −0.04; p = 0.034) were more pronounced in patients supplied with multiple-dose vitamin D than single-dose. Although the result of mortality showed no statistically significant effect, it indicated a reduced trend (OR 0.87, 95% CI 0.63, 1.12; p &gt; 0.05). The results of inflammatory markers reached no statistical differences.ConclusionThis meta-analysis revealed that moderate to severe COVID-19 patients supplied with multiple doses of vitamin D were less apt to need ICU admission, mechanical ventilation and have shorter hospital stays.
Evaluation of spine disorders using high contrast imaging of the cartilaginous endplate
Authors: Athertya Jiyo S. , Statum Sheronda , Chen Xiaojun , Du Kevin , Shin Soo Hyun , Jerban Saeed , Chung Christine B. , Chang Eric Y. , Ma Yajun 
Published: May 2024

Introduction: Many spine disorders are caused by disc degeneration or endplate defects. Because nutrients entering the avascular disc are channeled through the cartilaginous endplate (CEP), structural and compositional changes in the CEP may block this solute channel, thereby hindering disc cell function. Therefore, imaging the CEP region is important to improve the diagnostic accuracy of spine disorders.Methods: A clinically available T1-weighted and fat-suppressed spoiled gradient recalled-echo (FS-SPGR) sequence was optimized for high-contrast CEP imaging, which utilizes the short T1 property of the CEP. The FS-SPGR scans with and without breath-hold were performed for comparison on healthy subjects. Then, the FS-SPGR sequence which produced optimal image quality was employed for patient scans. In this study, seven asymptomatic volunteers and eight patients with lower back pain were recruited and scanned on a 3T whole-body MRI scanner. Clinical T2-weighted fast spin-echo (T2w-FSE) and T1-weighted FSE (T1w-FSE) sequences were also scanned for comparison.Results: For the asymptomatic volunteers, the FS-SPGR scans under free breathing conditions with NEX = 4 showed much higher contrast-to-noise ratio values between the CEP and bone marrow fat (BMF) (CNRCEP-BMF) (i.e., 7.8 ± 1.6) and between the CEP and nucleus pulposus (NP) (CNRCEP-NP) (i.e., 6.1 ± 1.2) compared to free breathing with NEX = 1 (CNRCEP-BMF: 4.0 ± 1.1 and CNRCEP-NP: 2.5 ± 0.9) and breath-hold condition with NEX = 1 (CNRCEP-BMF: 4.2 ± 1.3 and CNRCEP-NP: 2.8 ± 1.3). The CEP regions showed bright linear signals with high contrast in the T1-weighted FS-SPGR images in the controls, while irregularities of the CEP were found in the patients.Discussion: We have developed a T1-weighted 3D FS-SPGR sequence to image the CEP that is readily translatable to clinical settings. The proposed sequence can be used to highlight the CEP region and shows promise for the detection of intervertebral disc abnormalities.
The impact of social network use on adolescent depression: the chain mediation between self-objectification and body satisfaction
Authors: Tang Yiming , Xu Min , Tan Zhongwei , Liu Yong 
Published: May 2024

IntroductionAdolescents are in the transitional stage from childhood to adulthood, a critical period for individual physical and mental development. With the rapid development of the Internet, social networking has become an integral part of adolescents’ daily lives. However, the information that adolescents are exposed to on social networks is often processed and embellished, which may cause them to become physically dissatisfied and lead to emotional problems, such as depression. We investigated the chain-mediating effects of self-objectification and body satisfaction on the relationship between social network use and depression.MethodsWe utilized questionnaire data of 2025 adolescents from two secondary schools and one high school in China.ResultsOur results demonstrated that (1) there are obvious sex differences in the intensity of social network use and active and passive social network use among adolescents, with usage higher among girls than for boys; (2) self-objectification and body satisfaction play a mediating role in the relationship between the intensity of social network use and adolescent depression, as well as the presence of chain-mediating roles; and (3) self-objectification and body satisfaction play an intermediary role in the relationship between active and passive social network use and adolescent depression; there is further a chain intermediary role. The findings suggest that social network use affects adolescents’ depression through self-objectification and body satisfaction, which is not only manifested in the general use intensity of social networks but also in their active and passive use modes of social networks.ConclusionThis study provides theoretical support for the causes and mechanisms behind the influence of social network use on adolescent depression and has practical implications for the prevention and intervention of adolescent emotional problems.
Social anxiety mediates between victimization experiences and internet addiction among adolescents: results from propensity score matching
Authors: Wu Jianwei , Wang Hui , Li Xiang , Van Iat Kio , Xie Xuebin , Pang Ching Han 
Published: May 2024

BackgroundPrevious research has indicated that Victimization Experiences (VE) may be linked to a heightened likelihood of developing psychological symptoms and Internet Addiction (IA) among adolescents. However, the precise mechanism through which VE contributes to IA in adolescents remains uncertain. This study aimed to investigate whether Social Anxiety (SA) serves as a mediation between VE and IA, utilizing the framework of General Strain Theory.MethodsA cross-sectional survey among 11 middle schools or high schools in Macao was conducted from October to December 2022. Respondents in the victimized group and non-victimized group were 1:1 paired using Propensity Score Matching (PSM) to control the potential confounding factors.ResultsA total of 1,089 questionnaires were valid for analysis and 311 pairs were generated through PSM. Respondents in the victimized group reported significantly higher IA than those in non-victimized group (23.5% vs. 12.5%, p &lt; 0.001) after PSM treatment. Multivariate logistic regression analysis showed that VE (p = 0.015, OR = 1.750, 95% CI = 1.115 to 2.746, E-value = 2.90) and SA (p &lt; 0.001, OR = 1.052, 95% CI = 1.030 to 1.074, E-value = 1.29) were the predictors of IA. The model successfully classified 81.7% of cases overall (R2N = 0.133). Further analysis indicated that SA mediates between VE and IA (Z = 3.644, p &lt; 0.001).ConclusionThis study revealed the potential mediation effect of SA on the link between VE and IA. By acknowledging the mediating influence of SA, researchers and practitioners can develop more accurate and effective strategies to mitigate Internet Addiction among adolescents.
How expressive ties energize competitive performance in DanceSport dyads: unraveling the role of athlete engagement in an innovatively applied actor-partner interdependence mediation model
Authors: Liu Xiuxia , Liu Yang , Pan Dandan , Weng Xinghe 
Published: May 2024

ObjectivesThis study explores the significant impact of expressive ties (EI) between DanceSport couples on their competitive performance (CP). Utilizing a dyadic approach, we examined the performance achievement processes of DanceSport couples in relation to their EI.MethodsParticipants comprised 67 dyads of Chinese elite dancers aged between 16 and 30 years. The dyadic analysis was carried out using a structural equation model based on the actor-partner interdependence mediation model.ResultsWith regard to actor effects, both male (β  =  0.292, p =  0.012) and female (β  =  0.443, p &lt;  0.001) dancers’ perceived quality of EI had a positive correlation with CP. The males’ athlete engagement (AE) partially mediated the impact of EI on CP [indirect effect  =  0.144, SE  =  0.072, 95% confidence intervals (CI)  =  0.020, 0.283]. Regarding partner effects, females’ perceived EI quality positively influenced the male’s CP (β  =  0.26, p =  0.023) and mediated this association through the male’s AE [indirect effect  =  0.086, SE  =  0.041, 95% confidence intervals (CI)  =  0.003, 0.149]. Similarly, the females’ AE mediated the effect of males’ perceived EI quality on the females’ CP [indirect effect  =  0.152, SE  =  0.074, 95% confidence intervals (CI) =0.002, 0.256].ConclusionWe not only validated the propositions of the self-determination theory but also provided valuable insights to further enrich it. Our findings underscore that self-determination theory must account for individual gender characteristics.