bluuebunny/crossref_metadata_2025_split huggingface dataset:
Micro‐CT‐based bone ceramic scaffolding and its performance after seeding with mesenchymal stem cells for repair of load‐bearing bone defect in canine femoral head
Authors: Peng Jiang , Wen Cunyi , Wang Aiyuan , Wang Yu , Xu Wenjing , Zhao Bin , Zhang Li , Lu Shibi , Qin Ling , Guo Quanyi , Dong Limin , Tian Jiemo 
Published: January 2011

AbstractOsteonecrosis of the femoral head is a debilitating and painful orthopedic condition characterized by joint collapse. Salvage of the femoral head is highly desirable to preserve the contour and mechanical properties and prevent joint collapse. This study aimed to develop a new tissue‐engineering approach for treatment of large bone defect in femoral head, that is, after osteonecrosis. The biphasic calcium phosphate (BCP) ceramic scaffolds were fabricated by a 3D gel‐lamination technique based on micro‐computed tomography (micro‐CT) images of the cancellous bone microarchitecture of femoral heads. After seeding with autologous bone marrow‐derived mesenchymal stem cells (BMSCs) in vitro, the cell‐scaffold composite was implanted into a bone defect surgically induced in canine femoral head via trapdoor procedure, which was a common procedure for treatment of osteonecrosis. A total of 24 adult dogs were randomly divided into three groups (n = 8 each) for implantation of the BCP scaffold with or without with BMSCs, and also the autologous bone chips for comparisons. All animals were sacrificed at 30 weeks postoperatively and processed for radiological and histological evaluations. The contour of the femoral head was well preserved with implantation of BCP scaffolds with or without BMSCs, whereas joint collapse was found after treatment with autologous bone chips. The osteointegration and new bone formation was significantly greater with BCP scaffold implantation with than without BMSC seeding and showed greater strength and compressive modulus in the repair site. Micro‐CT‐based bone ceramic scaffolds seeding with BMSC might be a promising way to repair bone defects in the femoral head. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.
Work hours, retirement, and supportive relations among older adults
Authors: Nahum‐Shani Inbal , Bamberger Peter A. 
Published: January 2011

AbstractWhile the literature widely acknowledges the importance of social support to the health, well‐being, and performance of older adults, little is known about the way in which occupational conditions affect older employees' access to social support over time and whether these effects are maintained after retirement. Accordingly, in the current study we examine the degree to which work hours have longer term effects on the amount and type of support older individuals receive from intimate coworkers, family, and non‐work friends, and whether these effects are attenuated or intensified for those who retire. Longitudinal data were collected from a random sample of members of nine unions, six months prior to their retirement eligibility (T1) and approximately one year after Time 1 (T2). Our findings indicate that while retirement attenuates the positive association between Time 1 work hours and subsequent coworkers' support as well as the negative relationship between Time 1 work hours and subsequent non‐work friends support, retirement fails to attenuate the negative effect of Time 1 work hours on subsequent family support. Policy implications are discussed. Copyright © 2010 John Wiley & Sons, Ltd.
Vitamin B6 suppresses serine protease inhibitor 3 expression in the colon of rats and in TNF‐α‐stimulated HT‐29 cells
Authors: Yanaka Noriyuki , Ohata Tomomi , Toya Keigo , Kanda Mayumi , Hirata Atsuko , Kato Norihisa 
Published: January 2011

AbstractScope: Previous reports in the areas of animal studies and, recently epidemiology, have linked anti‐tumorigenic and anti‐inflammatory effects to dietary vitamin B6. This study investigated the molecular mechanism of these effects of vitamin B6.Methods and results: DNA microarray analysis was used to obtain information on changes in colon gene expression from vitamin B6 (pyridoxine) repletion in vitamin B6‐deficient rats. Pyridoxine supplementation down‐regulated the inflammatory molecule, serine protease inhibitor clade A member 3 (SPI‐3) mRNA expression in the colon. This study also showed that tumor necrosis factor α (TNF‐α) induced SPI‐3 mRNA expression in HT‐29 human colon cancer cells, and vitamin B6 (pyridoxal hydrochloride) pretreatment of HT‐29 cells inhibited TNF ‐induced mRNA expression of SPI‐3. Vitamin B6 inhibited TNF‐α‐induced NF‐κB activation via suppression of IκBα degradation in HT‐29 cells. HT‐29 cells stably expressing epitope‐tagged ubiquitin were generated and vitamin B6 pretreatment was shown to inhibit ubiquitination of the IkB protein in response to TNF‐α‐i.Conclusion: Vitamin B6 suppressed SPI‐3 expression in the colon of rats and in TNF‐α‐stimulated HT‐29 cells. Further, this study showed a possible role of vitamin B6 in the regulation of protein ubiquitination.
Lunasin induces apoptosis and modifies the expression of genes associated with extracellular matrix and cell adhesion in human metastatic colon cancer cells
Authors: Dia Vermont P. , de Mejia Elvira Gonzalez 
Published: January 2011

AbstractScope: Lunasin is an arginine‐glycine‐aspartic acid (RGD) cancer preventive peptide. The objective was to evaluate the potential of lunasin to induce apoptosis in human colon cancer cells and their oxaliplatin‐resistant (OxR) variants, and its effect on the expression of human extracellular matrix and adhesion genes.Methods and results: Various human colon cancer cell lines which underwent metastasis were evaluated in vitro using cell flow cytometry and fluorescence microscopy. Lunasin cytotoxicity to different colon cancer cells correlated with the expression of α5b1 integrin, being most potent to KM12L4 cells (IC50 = 13 μM). Lunasin arrested cell cycle at G2/M phase with concomitant increase in the expression of cyclin‐dependent kinase inhibitors p21 and p27. Lunasin (5–25 μM) activated the apoptotic mitochondrial pathway as evidenced by changes in the expressions of Bcl‐2, Bax, nuclear clusterin, cytochrome c and caspase‐3 in KM12L4 and KM12L4‐OxR. Lunasin increased the activity of initiator caspase‐9 leading to the activation of caspase‐3 and also modified the expression of human extracellular matrix and adhesion genes, downregulating integrin α5, SELE, MMP10, integrin β2 and COL6A1 by 5.01‐, 6.53‐, 7.71‐, 8.19‐ and 10.10‐fold, respectively, while upregulating COL12A1 by 11.61‐fold.Conclusion: Lunasin can be used in cases where resistance to chemotherapy developed.
Naturstoff‐Lego
Authors: Sundermann Uschi , Kushnir Susanna , Schulz Frank 
Published: December 2010

AbstractDie biosynthetische Modifikation von Polyketiden könnte einen Zugang zu großen Substanzbibliotheken dieser Naturstoffklasse öffnen. Mehrere Derivate natürlicher Polyketide entstanden so, wenn auch bisher zumeist nur in analytischen Mengen.
Fault detection for linear stochastic systems with sensor stuck faults
Authors: Li Xiao‐Jian , Yang Guang‐Hong 
Published: January 2011

SUMMARYThis paperaddresses the fault detection (FD) problem for Itô‐type linear stochastic systems with a class of sensor stuck faults and reference inputs. Considering all the possible sensor stuck faults, the stochastic systems are modeled via multi‐models, i.e. fault‐free model and faulty models. The main contributions are that a novel FD scheme in stochastic setting is proposed that is valid for arbitrary small sensor stuck faults including outage cases (the stuck values are zero). In addition, convex conditions for characterizing fault sensitivity performance and disturbance attenuation performance in stochastic sense are presented and these conditions include some previous results on deterministic systems as a special case. Finally, two examples are used to demonstrate the method and its effectiveness. Copyright © 2011 John Wiley & Sons, Ltd.
P‐selectin glycoprotein ligand‐1 modulates immune inflammatory responses in the enteric lamina propria
Authors: Nuñez‐Andrade Norman , Lamana Amalia , Sancho David , Gisbert Javier P , Gonzalez‐Amaro Roberto , Sanchez‐Madrid Francisco , Urzainqui Ana 
Published: January 2011

AbstractP‐selectin glycoprotein ligand‐1 (PSGL‐1), a leukocyte adhesion receptor that interacts with selectins, induces a tolerogenic programme in bone marrow‐derived dendritic cells (DCs), which in turn promotes the generation of T regulatory (Treg) lymphocytes. In the present study, we have used a mouse model of dextran sulphate sodium (DSS)‐induced colitis and studied the characteristics of the inflammatory cell infiltrate in the lamina propria (LP), mesenteric lymph nodes (mLNs) and Peyer's patches (PPs) to assess the possible role of PSGL‐1 in the modulation of the enteric immune response. We have found that untreated PSGL‐1‐deficient mice showed an altered proportion of innate and adaptive immune cells in mLNs and PPs as well as an activated phenotype of macrophages and DCs in the colonic LP that mainly produced pro‐inflammatory cytokines. Administration of an anti‐PSGL‐1 antibody also reduced the total numbers of macrophages, DCs and B cells in the colonic LP, and induced a lower expression of MHC‐II by DCs and macrophages. After DSS treatment, PSGL‐1−/− mice developed colitis earlier and with higher severity than wild‐type (WT) mice. Accordingly, the colonic LP of these animals showed an enhanced number of Th1 and Th17 lymphocytes, with enhanced synthesis of IL‐1α, IL‐6 and IL‐22, and increased activation of LP macrophages. Together, our data indicate that PSGL‐1 has a relevant homeostatic role in the gut‐associated lymphoid tissue under steady‐state conditions, and that this adhesion receptor is able to down‐regulate the inflammatory phenomenon in DSS‐induced colitis. Copyright © 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Maternal thyroid function at 11–13 weeks of gestation in fetal trisomies 21 and 18
Authors: Ashoor Ghalia , Maiz Nerea , Cuckle Howard , Jawdat Firas , Nicolaides Kypros H 
Published: January 2011

AbstractObjectiveTo examine the association between maternal serum levels of thyroid stimulating hormone (TSH) and free β‐human chorionic gonadotrophin (free β‐hCG) in trisomy 21, trisomy 18 and euploid pregnancies at 11–13 weeks and investigate the potential value of TSH in first‐trimester screening for aneuploidies.MethodsMaternal serum TSH and free β‐hCG levels at 11–13 weeks in 25 trisomy 21 and 25 trisomy 18 pregnancies were compared with levels in 3592 unaffected pregnancies. Only women with no history of thyroid disease and negative for antithyroid antibodies were included.ResultsSerum TSH in the trisomy 21 pregnancies was lower [0.76 multiples of the normal median (MoM), interquartile range (IQR) 0.46–1.09 MoM] and in trisomy 18 it was higher (1.25 MoM, IQR 0.88–1.98 MoM) than in unaffected pregnancies (1.01 MoM, IQR 0.61–1.51 MoM). There were significant associations between TSH and free β‐hCG in the unaffected pregnancies (r = − 0.214, p < 0.0001), but not in those with trisomy 21 (r = − 0.157, p = 0.452) or trisomy 18 (r = − 0.176, p = 0.401).ConclusionshCG, rather than TSH, may be the primary thyrotropic factor in early pregnancy. Measurement of TSH does not improve the performance of screening for trisomies 21 and 18 provided by nuchal translucency, free β‐hCG and pregnancy‐associated plasma protein‐A. Copyright © 2011 John Wiley & Sons, Ltd.
Prediction of early, intermediate and late pre‐eclampsia from maternal factors, biophysical and biochemical markers at 11–13 weeks
Authors: Akolekar Ranjit , Syngelaki Argyro , Sarquis Rita , Zvanca Mona , Nicolaides Kypros H. 
Published: January 2011

AbstractObjectiveTo develop models for prediction of pre‐eclampsia (PE) based on maternal factors and biophysical and biochemical markers at 11–13 weeks' gestation.MethodsScreening study of singleton pregnancies at 11–13 weeks including 752 (2.2%) that subsequently developed PE and 32 850 that were unaffected by PE. Models were developed for the prediction of early PE, requiring delivery before 34 weeks, intermediate PE with delivery at 34–37 weeks and late PE delivering after 37 weeks. The data used for the models were firstly, maternal characteristics and history, uterine artery pulsatility index, mean arterial pressure and serum pregnancy‐associated plasma protein‐A obtained from the screening study and secondly, maternal serum or plasma concentration of placental growth factor, placental protein‐13, inhibin‐A, activin‐A, soluble endoglin, pentraxin‐3 and P‐selectin obtained from case‐control studies.ResultsIn screening for PE by maternal factors only at a fixed false positive rate of 5%, the estimated detection rates were 33.0% for early PE, 27.8% for intermediate PE and 24.5% for late PE. The respective detection rates in screening by a combination of maternal factors, biophysical and biochemical markers were 91.0, 79.4 and 60.9%.ConclusionsEffective prediction of PE can be achieved at 11–13 weeks' gestation. Copyright © 2011 John Wiley & Sons, Ltd.
Efficient light trapping in silicon solar cells by ultrafast‐laser‐induced self‐assembled micro/nano structures
Authors: Nayak Barada K. , Iyengar Vikram V. , Gupta Mool C. 
Published: January 2011

AbstractA novel ultrafast laser processing technique is used to create self‐assembled micro/nano structures on a silicon surface for efficient light trapping. Under appropriate experimental conditions, light reflection (including scattering) of the Si surface has been reduced to less than 3% for the entire solar spectrum and the material appears completely black to the naked eye. A post‐chemical cleaning is applied to remove laser‐redeposited material and induced defects. Optical, morphological, and structural characterizations have been carried out on as‐laser‐treated and post‐chemically cleaned surfaces. Finally, we report for the first time the total efficiency of over 14% and high external quantum efficiency (EQE) results on photovoltaic devices fabricated on the ultrafast‐laser‐induced micro/nano structured silicon wafer, which can be further improved upon process optimization. Copyright © 2011 John Wiley & Sons, Ltd.
New world record efficiency for Cu(In,Ga)Se2 thin‐film solar cells beyond 20%
Authors: Jackson Philip , Hariskos Dimitrios , Lotter Erwin , Paetel Stefan , Wuerz Roland , Menner Richard , Wischmann Wiltraud , Powalla Michael 
Published: January 2011

AbstractIn this contribution, we present a new certified world record efficiency of 20.1 and 20.3% for Cu(In,Ga)Se2 thin‐film solar cells. We analyse the characteristics of solar cells on such a performance level and demonstrate a high degree of reproducibility. Copyright © 2011 John Wiley & Sons, Ltd.
Analysis of degradation mechanisms of crystalline silicon PV modules after 12 years of operation in Southern Europe
Authors: Sánchez‐Friera Paula , Piliougine Michel , Peláez Javier , Carretero Jesús , Sidrach de Cardona Mariano 
Published: January 2011

AbstractThe long‐term reliability of photovoltaic modules is crucial to ensure the technical and economic viability of PV as a successful energy source. The analysis of degradation mechanisms of PV modules is key to ensure current lifetimes exceeding 25 years. This paper presents the results of the investigations carried out on the degradation mechanisms of a crystalline silicon PV installation of 2 kWp after 12 years of exposure in Málaga, Spain. The analysis was conducted by visual inspection, infrared thermography and electrical performance evaluation. By visual inspection, the most relevant defects in the modules were identified and ranked according to their frequency. The electrical performance was assessed by comparing the characteristic parameters of the individual modules, obtained by outdoor measurements at the start and end of the exposure period. The correlation of the visual defects and the shifts in the electrical parameters was analysed. The results presented show that glass weathering, delamination at the cell‐EVA interface and oxidation of the antireflective coating and the cell metallization grid were the most frequently occurring defects found. The total peak power loss, including the initial light induced degradation, was 11.5%, which corresponded almost totally to a loss in short‐circuit current. Copyright © 2011 John Wiley & Sons, Ltd.
Upper limits to absorption enhancement in thick solar cells using diffraction gratings
Authors: Mellor A. , Tobías I. , Martí A. , Mendes M.J. , Luque A. 
Published: January 2011

AbstractThe application of diffraction gratings to solar cells is a promising approach to superseding the light trapping limits of conventional Lambertian structures. In this paper a mathematical formalism is derived for calculating the absorption that can be expected in a solar cell equipped with a diffraction grating, which can be applied to any lattice geometry and grating profile. Furthermore, the formalism is used to calculate the upper limit of total absorption that can theoretically be achieved using a diffraction grating. The derived formalism and limits are valid when the solar cell thickness is greater than the coherence length of the illuminating solar spectrum. Comparison is made to the upper limit achievable using an angularly selective Rugate filter, which is also calculated. Both limits are found to be considerably higher than the Lambertian limit within the range of sunlight concentration factors practically employed in photovoltaic systems (1–1000×). The upper limit of absorption using the diffraction grating is shown to be equal to the thermodynamic limit for all absorbances and concentration factors. The limit for the Rugate filter is generally lower, but tends to the thermodynamic limit for lower cell absorbances. Copyright © 2011 John Wiley & Sons, Ltd.
Synthesis, Structure, and Physical Properties of a Barium Complex with 5‐Sulfoisophthalic Acid Sodium Salt
Authors: Wu Gang , Yin Fun‐Jun , Wei Hao , Liu Zhen‐Feng , Wang Xiao‐Feng 
Published: January 2011

AbstractThe complex [Ba3(sip)2(H2O)9]·H2O (1) (NaH2sip = 5‐sulfoisophthalic acid sodium) was synthesized and characterized by single‐crystal X‐ray diffraction. Structural determination reveals that the asymmetric unit in 1 contains two crystallographically independent BaII atoms. The Ba1 atom is eight‐coordinate with distorted monocapped pentagonal bipyramidal arrangement, whereas the Ba2 atom is ten‐coordinated with bicapped tetragonal prismatic arrangement. The two carboxylate groups of sip3– adopt different coordination modes, μ2‐η1:η1‐bridging, and μ2‐η2:η1‐bridging. The sulfonate group coordinates to three different BaII atoms in a tridentate μ3 mode to generate a ladder‐like one‐dimensional chain. The chains are connected by μ2‐η1:η1‐bridging carboxylate groups to form a wave‐like two‐dimensional network, which are further linked by sip3– anions to generate a three‐dimensional structure. The thermal stability and luminescence properties of complex 1 were also investigated.
Anti-metabolic syndrome and immunostimulant activities of Egyptian fenugreek seeds in diabetic/obese and immunosuppressive rat models
Authors: Ramadan Gamal , El-Beih Nadia M. , Abd El-Kareem Hanaa F. 
Published: January 2011

Preliminary trials have suggested possible hypoglycaemic, hypolipidaemic and immunomodulatory properties of the fenugreek plant. Here, we evaluated and compared the efficacy of Egyptian fenugreek seed powder (FSP, 0·5 and 1·0 g/kg body weight) in alleviating the experimentally induced metabolic syndrome (in type 1 diabetic and obese rat models) and experimentally induced immunosuppression and delay in burn-healing (in cyclophosphamide (CP)-treated rats). FSP significantly alleviated (P < 0·05–0·001) most signs of the metabolic syndrome resulting from experimentally induced type 1 diabetes and obesity by 40–76 and 56–78 %, respectively, including hyperglycaemia, hyperlipidaemia, elevation in atherogenic indices, impairment of liver functions, severe changes in body weight and oxidative stress. Besides, FSP (especially the high dose) completely modulated the immunosuppressive activity of CP including leucopenia (resulting from neutropenia and lymphopenia), decrease in weights and cellularity of lymphoid organs, serum γ-globulin level, delayed type of hypersensitivity response and delay in the skin-burning healing process. FSP decreased the immunosuppressive activity of CP by 57–108 %. These beneficial effects of FSP were dose dependent in most cases, and FSP doses used here were considered safe in general. FSP was more efficient in alleviating the signs of the metabolic syndrome in the obese animals (over 9 %) than in the type 1 diabetic animals. Moreover, the immunostimulant activity of fenugreek seeds exceeded their anti-metabolic syndrome activity by 15–24 %. In conclusion, fenugreek seeds may be useful not only as a dietary adjunct for the control of the metabolic syndrome in diabetic/obese patients, but also as an immunostimulant in immunocompromised patients such as those under chemotherapeutic interventions.
Iron deficiency up-regulates iron absorption from ferrous sulphate but not ferric pyrophosphate and consequently food fortification with ferrous sulphate has relatively greater efficacy in iron-deficient individuals
Authors: Zimmermann Michael B. , Biebinger Ralf , Egli Ines , Zeder Christophe , Hurrell Richard F. 
Published: January 2011

Fe absorption from water-soluble forms of Fe is inversely proportional to Fe status in humans. Whether this is true for poorly soluble Fe compounds is uncertain. Our objectives were therefore (1) to compare the up-regulation of Fe absorption at low Fe status from ferrous sulphate (FS) and ferric pyrophosphate (FPP) and (2) to compare the efficacy of FS with FPP in a fortification trial to increase body Fe stores in Fe-deficient childrenv. Fe-sufficient children. Using stable isotopes in test meals in young women (n49) selected for low and high Fe status, we compared the absorption of FPP with FS. We analysed data from previous efficacy trials in children (n258) to determine whether Fe status at baseline predicted response to FSv. FPP as salt fortificants. Plasma ferritin was a strong negative predictor of Fe bioavailability from FS (P < 0·0001) but not from FPP. In the efficacy trials, body Fe at baseline was a negative predictor of the change in body Fe for both FPP and FS, but the effect was significantly greater with FS (P < 0·01). Because Fe deficiency up-regulates Fe absorption from FS but not from FPP, food fortification with FS may have relatively greater impact in Fe-deficient children. Thus, more soluble Fe compounds not only demonstrate better overall absorption and can be used at lower fortification levels, but they also have the added advantage that, because their absorption is up-regulated in Fe deficiency, they innately ‘target’ Fe-deficient individuals in a population.