bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of arachidonic acid in ischemic heart disease under different comorbidities: risk or protection?
Authors: Li Chengjia , Chen Huijun 
Published: June 2024

AbstractIn a translational study involving animal models and human subjects, Lv et al. demonstrate that arachidonic acid (AA) exhibits cardioprotective effects in diabetic myocardial ischemia, suggesting a departure from its known role in promoting ferroptosis—a form of cell death characterized by iron-dependent lipid peroxidation. However, the study does not address how underlying diabetic conditions might influence the metabolic pathways of AA, which are critical for fully understanding its impact on heart disease. Diabetes can significantly alter lipid metabolism, which in turn might affect the enzymatic processes involved in AA’s metabolism, leading to different outcomes in the disease process. Further examination of the role of diabetes in modulating AA’s effects could enhance the understanding of its protective mechanism in ischemic conditions. This could also lead to more targeted and effective therapeutic strategies for managing myocardial ischemia in diabetic patients, such as optimizing AA levels to prevent heart damage while avoiding exacerbating factors like ferroptosis.
Assessment of inbreeding depression on morphometric traits among North Indian population cohorts
Authors: Fatma Rafat , Chauhan Waseem , Afzal Mohammad 
Published: June 2024

Abstract
Aim
Inbreeding is thought to affect the morphometric parameters leading to lower health status among the progeny. The present study was aimed to investigate the repercussions of inbreeding on anthropometric traits, namely height, weight and body mass index (BMI).

Subjects and methods
The survey was conducted in two North Indian cities and total 813 individuals were randomly recruited from inbred and outbred families. The morphometric parameters of the subjects were measured using standard methods, BMI was calculated and categorized into underweight, normal weight and overweight. Family pedigrees were drawn and degree of inbreeding in terms of the inbreeding coefficient (F) was calculated.

Results
A significant decline in morphometric measures was observed among inbred individuals as compared to outbred ones. The mean differences (95% CI) were found significant for various inbred and first cousin categories as compared to outbred subjects (p < 0.05). We found the increased frequency of underweight individuals corresponding to the degree of inbreeding for different types of inbred categories (p = 2.086 × 10−9) and also for different subtypes of first cousin unions corresponding to their sex-linked inbreeding coefficients (p = 5.2 × 10−5). The regression slope and correlation coefficient revealed a fitness decline and depression in anthropometric measures (p < 0.05) with the increase in ‘F’ for all inbred groups and first cousin categories.

Conclusions
The present research confirms the adverse effects of inbreeding on morphometric parameters among inbred subjects. It has novelty in shedding light on the hitherto unreported differences in the consequences of inbreeding among different types of first cousin unions.
Changes in glucose metabolism, C-reactive protein, and liver enzymes following intake of NAD + precursor supplementation: a systematic review and meta‐regression analysis
Authors: Sohouli Mohammad Hassan , Tavakoli Sogand , Reis Marcela Gomes , Hekmatdoost Azita , Guimarães Nathalia Sernizon 
Published: June 2024

Abstract
Background
There are contradictory effects regarding the effect of NAD + precursor on glucose metabolism and liver enzymes. In order to obtain a better viewpoint from them, this study aimed to comprehensively investigate the effects of NAD + precursor supplementation on glucose metabolism, C-reactive protein (CRP), and liver enzymes.

Methods
PubMed/MEDLINE, Web of Science, SCOPUS, and Embase databases were searched using standard keywords to identify all controlled trials investigating the glucose metabolism, CRP, and liver enzymes effects of NAD + precursor. Pooled weighted mean difference (WMD) and 95% confidence intervals (95% CI) were achieved by random-effects model analysis for the best estimation of outcomes.

Results
Forty-five articles with 9256 participants’ were included in this article. The pooled findings showed that NAD + precursor supplementation had a significant increase in glucose (WMD: 2.17 mg/dL, 95% CI: 0.68, 3.66, P = 0.004) and HbA1c (WMD: 0.11, 95% CI: 0.06, 0.16, P < 0.001) as well as a significant decrease in CRP (WMD: -0.93 mg/l, 95% CI -1.47 to -0.40, P < 0.001) compared with control group, and was not statistically significant with respect to insulin and homeostasis model assessment of insulin resistance (HOMA-IR). However, we found no systemic changes in aspartate transaminase (AST), alanine transaminase (ALT), or alkaline phosphatase (ALP) levels after NAD + precursor supplementation. The results of the subgroup analysis showed that the intake of NAD + precursor during the intervention of more than 12 weeks caused a greater increase in the glucose level. Furthermore, Nicotinic acid supplementation (NA) causes a greater increase in glucose and HbA1c levels than nicotinamide (NE) supplementation.

Conclusions
Overall, these findings suggest that NAD + precursor supplementation might have an increase effect on glucose metabolism as well as a decrease in CRP.
Anti-acetylated-tau immunotherapy is neuroprotective in tauopathy and brain injury
Authors: Parra Bravo Celeste , Krukowski Karen , Barker Sarah , Wang Chao , Li Yaqiao , Fan Li , Vázquez-Rosa Edwin , Shin Min-Kyoo , Wong Man Ying , McCullough Louise D. , Kitagawa Ryan S. , Choi H. Alex , Cacace Angela , Sinha Subhash C. , Pieper Andrew A. , Rosi Susanna , Chen Xu , Gan Li 
Published: June 2024

Abstract
Background
Tau is aberrantly acetylated in various neurodegenerative conditions, including Alzheimer’s disease, frontotemporal lobar degeneration (FTLD), and traumatic brain injury (TBI). Previously, we reported that reducing acetylated tau by pharmacologically inhibiting p300-mediated tau acetylation at lysine 174 reduces tau pathology and improves cognitive function in animal models.

Methods
We investigated the therapeutic efficacy of two different antibodies that specifically target acetylated lysine 174 on tau (ac-tauK174). We treated PS19 mice, which harbor the P301S tauopathy mutation that causes FTLD, with anti-ac-tauK174 and measured effects on tau pathology, neurodegeneration, and neurobehavioral outcomes. Furthermore, PS19 mice received treatment post-TBI to evaluate the ability of the immunotherapy to prevent TBI-induced exacerbation of tauopathy phenotypes. Ac-tauK174 measurements in human plasma following TBI were also collected to establish a link between trauma and acetylated tau levels, and single nuclei RNA-sequencing of post-TBI brain tissues from treated mice provided insights into the molecular mechanisms underlying the observed treatment effects.

Results
Anti-ac-tauK174 treatment mitigates neurobehavioral impairment and reduces tau pathology in PS19 mice. Ac-tauK174 increases significantly in human plasma 24 h after TBI, and anti-ac-tauK174 treatment of PS19 mice blocked TBI-induced neurodegeneration and preserved memory functions. Anti-ac-tauK174 treatment rescues alterations of microglial and oligodendrocyte transcriptomic states following TBI in PS19 mice.

Conclusions
The ability of anti-ac-tauK174 treatment to rescue neurobehavioral impairment, reduce tau pathology, and rescue glial responses demonstrates that targeting tau acetylation at K174 is a promising neuroprotective therapeutic approach to human tauopathies resulting from TBI or genetic disease.
Individualized dynamic frailty-tailored therapy (DynaFiT) in elderly patients with newly diagnosed multiple myeloma: a prospective study
Authors: Zhang Yingjie , Liang Xinyue , Xu Weiling , Yi Xingcheng , Hu Rui , Ma Xintian , Yan Yurong , Zhang Nan , Wang Jingxuan , Sun Xiaoxiao , Zhu Yufeng , Tian Mengru , Lan Maozhuo , Long Mengtuan , Dai Yun , Jin Fengyan 
Published: June 2024

AbstractIt remains a substantial challenge to balance treatment efficacy and toxicity in geriatric patients with multiple myeloma (MM), primarily due to the dynamic nature of frailty. Here, we conducted a prospective study to evaluate the feasibility and benefits of dynamic frailty-tailored therapy (DynaFiT) in elderly patients. Patients with newly diagnosed MM (aged ≥ 65 years) received eight induction cycles of bortezomib, lenalidomide, and dexamethasone (daratumumab was recommended for frail patients), with treatment intensity adjusted according to longitudinal changes in the frailty category (IMWG-FI) at each cycle. Of 90 patients, 33 (37%), 16 (18%), and 41 (45%) were fit, intermediate fit, and frail at baseline, respectively. Of 75 patients who had geriatric assessment at least twice, 28 (37%) experienced frailty category changes at least once. At analysis, 15/26 (58%) frail patients improved (27% became fit and 31% became intermediate fit), 4/15 (27%) intermediate fit patients either improved or deteriorated (two for each), and 6/30 (20%) fit patients deteriorated. During induction, 34/90 (38%) patients discontinued treatment, including 10/33 (30%) fit, 4/16 (25%) intermediate fit, and 20/41 (49%) frail; 14/40 (35%) frail patients discontinued treatment within the first two cycles, mainly because of non-hematologic toxicity (mostly infections). For fit, intermediate-fit, and frail patients, the overall response rate was 100%, 93%, and 73%, respectively; one-year overall survival was 90%, 75%, and 54%, respectively. Therefore, the individualized DynaFiT is feasible and promising for heterogeneous elderly patients.
Silver diamine fluoride for the management of dental caries in children in primary dental care: protocol for a feasibility study
Authors: Timms Laura , Rodd Helen , Deery Chris , Brocklehurst Paul , Marshman Zoe 
Published: June 2024

Abstract
Background
Dental caries remains a significant problem in England, affecting 11% of 3-year-olds and 23% of 5-year-olds. While current approaches have been extensively investigated, their ability to (1) control pain and infection; (2) prevent hospital admissions, and (3) be implemented within the National Health Service (NHS) contractual arrangements, remains unsatisfactory. Silver diamine fluoride (SDF) is an alternative, non-invasive approach that has proven efficacy in arresting caries progression in primary teeth, principally from studies conducted outside of Europe. Its use in primary dental care in the UK is limited, despite the acknowledged need.
The clinical and cost-effectiveness of SDF has not been compared to usual care in the UK. Before a pragmatic randomised controlled trial (RCT) can be conducted to compare SDF to usual care for caries management in young children, there are several uncertainties that require investigation. This study aims to establish whether such an RCT is feasible.

Methods
This mixed-method parallel design study is a feasibility study with an embedded process evaluation, to compare SDF with usual treatment in primary dental care in the UK. It will be individually randomised, with 13 dentists and therapists, in 8 different dental primary care sites with a sample size of 80 child participants aged 1–8 years old. The aim will be to recruit ten participants per site with equal arm allocation. Follow-up will be for 1 year. The study will inform whether an RCT is feasible by resolving several key uncertainties. The acceptability and implementation of SDF and the research processes will be explored. Patient and Public Involvement and Engagement representatives will be involved throughout recruitment and retention strategies, participant documentation, analysis, engagement and dissemination.

Discussion
The ability to conduct an RCT will be evaluated. If feasible, this RCT has the potential to evaluate the effectiveness of a non-invasive approach for the management of untreated caries in young children. A feasibility study also offers the opportunity to consider factors associated with the implementation of SDF at an early stage through a process evaluation that will inform the definitive trial and an implementation strategy for SDF by identifying relevant barriers and facilitators.

Trial registration
ClinicalTrials.gov identifier: NCT06092151. Date: 19/10/2023.
Gender differences in survival across the ages of life: an introduction
Authors: Zarulli Virginia , Salinari Giambattista 
Published: June 2024

AbstractThis thematic series explores the complex nature of the survival gap between genders across the lifespan. It highlights how, although women generally have a longer life expectancy than men globally, the complexity of this advantage challenges simplistic explanations. The text emphasizes several areas of interest, such as mortality differences between genders at different life stages, the aging process, and epidemiological differences between the sexes. Additionally, it discusses the role of biological and socio-behavioral factors in explaining the female survival advantage and how this gap has evolved and been influenced by historical, behavioral, and lifestyle changes. With six compelling articles from diverse disciplinary angles, this series underscores the interdisciplinary approach essential for unraveling the intricacies of gender differences in survival across the lifespan.
Comparing machine learning algorithms to predict vegetation fire detections in Pakistan
Authors: Shahzad Fahad , Mehmood Kaleem , Hussain Khadim , Haidar Ijlal , Anees Shoaib Ahmad , Muhammad Sultan , Ali Jamshid , Adnan Muhammad , Wang Zhichao , Feng Zhongke 
Published: June 2024

AbstractVegetation fires have major impacts on the ecosystem and present a significant threat to human life. Vegetation fires consists of forest fires, cropland fires, and other vegetation fires in this study. Currently, there is a limited amount of research on the long-term prediction of vegetation fires in Pakistan. The exact effect of every factor on the frequency of vegetation fires remains unclear when using standard analysis. This research utilized the high proficiency of machine learning algorithms to combine data from several sources, including the MODIS Global Fire Atlas dataset, topographic, climatic conditions, and different vegetation types acquired between 2001 and 2022. We tested many algorithms and ultimately chose four models for formal data processing. Their selection was based on their performance metrics, such as accuracy, computational efficiency, and preliminary test results. The model’s logistic regression, a random forest, a support vector machine, and an eXtreme Gradient Boosting were used to identify and select the nine key factors of forest and cropland fires and, in the case of other vegetation, seven key factors that cause a fire in Pakistan. The findings indicated that the vegetation fire prediction models achieved prediction accuracies ranging from 78.7 to 87.5% for forest fires, 70.4 to 84.0% for cropland fires, and 66.6 to 83.1% for other vegetation. Additionally, the area under the curve (AUC) values ranged from 83.6 to 93.4% in forest fires, 72.6 to 90.6% in cropland fires, and 74.2 to 90.7% in other vegetation. The random forest model had the highest accuracy rate of 87.5% in forest fires, 84.0% in cropland fires, and 83.1% in other vegetation and also the highest AUC value of 93.4% in forest fires, 90.6% in cropland fires, and 90.7% in other vegetation, proving to be the most optimal performance model. The models provided predictive insights into specific conditions and regional susceptibilities to fire occurrences, adding significant value beyond the initial MODIS detection data. The maps generated to analyze Pakistan’s vegetation fire risk showed the geographical distribution of areas with high, moderate, and low vegetation fire risks, highlighting predictive risk assessments rather than historical fire detections.
High-precision services of BeiDou navigation satellite system (BDS): current state, achievements, and future directions
Authors: GAO Weiguang , Zhou Wei , Tang Chengpan , Li Xingxing , Yuan Yongqiang , Hu Xiaogong 
Published: June 2024

AbstractWith the rapid development of technologies such as autonomous driving and robotic navigation, the demand for accurate and reliable positioning results with BDS has increased. The current status and future development of BDS high precision services are reviewed. The operational PPP-B2b of BDS-3 achieves positioning accuracy at decimeter-level within 14 min and has the drawbacks in regional coverage and long convergence time compared with the international counterparts, such as CLAS of QZSS and HAS of Galileo. A development frame for multi-layer BDS high-precision services is proposed by considering its construction cost, compatibility of user terminal, operating maintenance burden, and service level. The global high-precision service with the improved orbit and clock accuracy enhanced with the inter-satellite link is taken as the most fundamental layer. By incorporating inter-satellite link observations, the orbit errors URE and clock errors are significantly reduced to approximately 0.05 m and 0.16 ns, respectively, which will expand PPP service to global. Based on this, the regional PPP-AR and PPP-RTK services with improved convergence performance are taken as the second layer. With PPP-AR, the convergence time drops to 10 min. With PPP-RTK, the convergence time would be further shortened to less than 3 min. The LEO enhanced PPP service is taken as the third layer which overcomes both drawbacks of the long convergence time of the inter-satellite link enhanced service (the first layer) and the regional coverage of the PPP-AR/PPP-RTK services (the second layer). The simulation results show that by introducing a LEO constellation of 288 satellites, the LEO enhanced PPP can achieve positioning accuracy better than 5 cm within approximately 1 min. In addition, the integration of LEO constellation and PPP-RTK is further proposed to enable instantaneous convergence. The implementation challenges are also presented.
Novel triplex nucleic acid lateral flow immunoassay for rapid detection of Nipah virus, Middle East respiratory syndrome coronavirus and Reston ebolavirus
Authors: Chingtham Santhalembi , Kulkarni Diwakar D. , Sivaraman Sumi , Mishra Anamika , Pateriya Atul K. , Singh Vijendra Pal , Raut Ashwin Ashok 
Published: June 2024

AbstractWe report the development of a triplex nucleic acid lateral flow immunoassay (NALFIA) for the detection of the genomes of Nipah virus (NiV), Middle East respiratory syndrome coronavirus (MERS-CoV) and Reston ebolavirus (REBOV), which are intended for screening bats as well as other hosts and reservoirs of these three viruses. Our triplex NALFIA is a two-step assay format: the target nucleic acid in the sample is first amplified using tagged primers, and the tagged dsDNA amplicons are captured by antibodies immobilized on the NALFIA device, resulting in signal development from the binding of a streptavidin-colloidal gold conjugate to a biotin tag on the captured amplicons. Triplex amplification of the N gene of NiV, the UpE gene of MERS-CoV, and the Vp40 gene of REBOV was optimized, and three compatible combinations of hapten labels and antibodies were identified for end point detection. The lowest RNA copy numbers detected by the triplex NALFIA were 8.21e4 for the NiV N target, 7.09e1 for the MERS-CoV UpE target, and 1.83e4 for the REBOV Vp40 target. Using simulated samples, the sensitivity and specificity for MERS-CoV and REBOV targets were estimated to be 100%, while the sensitivity and specificity for the NiV target were 91% and 93.3%, respectively. The compliance rate between triplex NALFIA and real-time RT‒PCR was 92% for the NiV N target and 100% for the MERS-CoV UpE and REBOV Vp40 targets.
Testosterone and Erythrocyte Lifespan
Authors: McMartin Melissa C , Savkovic Sasha , Romano Adelina , Lim Sarina , Muir Christopher A , Jayadev Veena , Conway Ann J , Seccombe Leigh , Handelsman David J 
Published: June 2024

Abstract

Context
Endogenous and exogenous androgens increase circulating erythrocytes and hemoglobin but their effects on erythrocyte lifespan is not known.

Objective
To investigate androgen effects on immature and mature erythrocyte lifespan in humans and mice using novel nonradioactive minimally invasive methods.

Design
Human erythrocyte lifespan was estimated using alveolar carbon monoxide concentration and blood hemoglobin in Levitt's formula in hypogonadal or transgender men before and up to 18 weeks after commencing testosterone (T) treatment. Erythrocyte lifespan was estimated in androgen receptor knockout and wild-type mice after T or DHT treatment of intact females or orchidectomized males using in vivo biotin labelling of erythrocyte surface epitopes for reticulocytes (Ter119+CD71+) and 2 markers of erythrocytes (CD45–, Ter119+CD71–) monitoring their blood disappearance rate by flow cytometry.

Results
Before treatment, hypogonadal and transgender men had marked reduction in erythrocyte lifespan compared with controls. T treatment increased erythrocyte lifespan at 6 weeks but returned to pretreatment levels at 18 weeks, whereas serum T and blood hemoglobin were increased by T treatment remaining elevated at 18 weeks. In mice, T and DHT treatment had higher erythrocyte (but not reticulocyte) lifespan but neither orchidectomy nor androgen receptor inactivation significantly influenced erythrocyte or reticulocyte lifespan.

Conclusion
We conclude that hypogonadal men have reduced erythrocyte lifespan and acute androgen-induced increase in circulating erythrocyte lifespan may contribute to the well-known erythropoietic effects of androgens, but longer term effects require further investigation to determine how much they contribute to androgen-induced increases in circulating hemoglobin.
Preliminary Notes toward a Destituent Art
Authors: Petrossiants Andreas 
Published: June 2024

Abstract
This article argues that, by looking to the generalization of a certain kind of artistic or creative character in paradigms of work, there is a view onto a different kind of relationship between culture and militant action and fugitivity. Here, the term destituent art is proposed to sketch out a form of cultural production that does or might lay waste not only to institutional targets of critique but also to the structurally immanent belief in institutional reform that underlies the aesthetics of that critique. It is an invitation to reconsider some key myths that structure art discourse (even if they appear as objects of critique) and an appeal to read that discursivity as not just productive but potentially destituent. To do so, the author reads operaismo's rereading of Marx against Marxism alongside theorizations of destituent insurrection through the subject of the artist. Some recent social movements in New York City targeting art institutions are analyzed to consider this connection. Like Colectivo Situaciones’ discussion of destituent insurrection as a grammar that unifies surplus populations through their expressions of refusal (the riot, the strike, the commune) that are simultaneously modes of reorganizing forms of life, a destituent art would not signal a turn to a productive pessimism—that all creative work is already subsumed—but that the cultivation of destituent power actualizes and is actualized by the aesthetic production of places of action.