bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Follicular Fluid on Intracellular Free Calcium Levels in Human Spermatozoa
Authors: Fukui Keisuke , Takeda Yasunari , Yano Juri , Kaura Rumiko , Kitagawa Hiroyuki , Suginami Hirosi , Matsuura Shumpei , Oka Keizo 
Published: August 2010

AbstractIn this study, we demonstrate the effect of follicular fluid (FF) on free cytosolic calcium ion ([Ca2+]i) in human spermatozoa, using a fluorescent indicator “fura 2,” and we evaluate the factors that increase [Ca2+]i in FF. A significant and immediate [Ca2+]i increase (656.3 ± 148.6 nM) was elicited by intact FF. The increase in [Ca2+]i elicited by lipid‐stripped FF was only 50% (322 ± 68.7 nM) of that elicited by intact FF. However, 82% of the [Ca2+]i increase elicited by intact FF was observed when lipid‐stripped FF was supplemented with progesterone, but not when it was supplemented with estradiol. Also, progesterone at the concentrations of 1 ng/ml to 1 μg/ml caused a significant increase in [Ca2+]i by itself, but estradiol produced a small effect, ranging from 3% to 9% of the effect produced by progesterone. These results indicate that progesterone plays a major role in the lipid fraction in FF to elicit the entry of calcium into human spermatozoa.
Detection and Identification of Amines in Bacterial Vaginosis
Authors: Kubota Takeyoshi , Sakae Utsuno , Takeuchi Hisaya , Usui Mitsugu 
Published: August 2010

AbstractFor the purpose of determining an objective indicator of bacterial vaginosis (BV), we tried to detect and to identify amines in the vaginal fluid of patients by using the dansyl method (to detect amine in a solution of water). Bacteria isolated from these patients were also analyzed.In BV (n: 32) 7 kinds of amines were detected; and the positive rate of detection of the dansyl method was 90.6%. No specific bacteria was found in BV.The positive rate was 80.0% for the patients with trichomonas vaginitis (n: 5).The positive rate of detection was 20.0% in the candida group (n: 5), and 28.6% in the control group (n: 14). Those amines detected in other than BV‐related vaginitis are presumed not to vaporize. For purposes of diagnosis, it would be better to detect vaporized amines instead of detecting amines dissolved in vaginal fluid.
Bone Mineral Density in Premenopausal Anovulatory Women
Authors: Park Ki H. , Song Chan H. 
Published: August 2010

AbstractTo examine the impact of amenorrhea on bone mineral density in women of reproductive age, we performed a cross sectional study in 190 amenorrheic women, comprising 113 premature ovarian failure, 27 primary hypothalamic amenorrhea, 38 hyperprolactinemia and 12 Sheehan's syndrome.Measurement of bone mineral density was carried out using dual photon absorptiometry at four sites: femur neck, ward's triangle, trochanter and spine (L2–4) and we compared the result with that of 163 normal‐cycle control women with age matched.Bone mineral densities was significantly low in patients with primary hypothalamic amenorrhea and premature ovarian failure at all four sites. In those with hyperprolactinemia a decrease in bone mineral density was noted at femur neck and spine while those with Sheehan's syndrome is associated with decreased bone density at femur neck, ward's triangle and trochanter. The degree of bone loss and the affected sites seems differ depending on the type of amenorrhea. In patients with primary hypothalamic amenorrhea, significant bone loss was already noted at all four sites by age 20. The decrement in bone density continued rapidly during the early twenties up to age 25 and then slowed after age 25. In those with premature ovarian failure with secondary amenorrhea there were no decrease in bone mineral density within the first year after onset of amenorrhea, however, in the subsequent 2 years, the reduction in BMD was rapid and thereafter the reduction slowed remarkably. The BMD in this study positively correlated with the body mass index and negatively with the phosphorus intake.
Practical Management of Postmenopausal Women with Low Bone Mineral Density
Authors: Taga Michiyoshi 
Published: August 2010

AbstractFor the management of postmenopausal women with low bone mineral density (BMD), the gynecologist, as a primary care physician, should play a central role in preventing osteoporotic fracture by educating them for elimination of risk factors and for healthy life‐style and by providing an appropriate drug therapy. Because the risk of fracture usually increases after BMD decreases as much as 20–30% of peak bone mass, it is practically reasonable to set this level of BMD as a criteria for the initiation of management. As for a drug treatment, it is better to combine estrogen, the first choice drug, with progestogens to counteract any neoplastic effect of unopposed estrogen on the endometrium. Bisphosphonates and calcitonin, which are inhibitors of bone resorption, provide an alternative in prevention and treatment of postmenopausal bone loss in case of contraindication for estrogen or in those women who are unwilling to take estrogen.
Detection of Lymph Node Metastasis in Ovarian Carcinoma and Uterine Corpus Carcinoma by Preoperative Computerized Tomography or Magnetic Resonance Imaging
Authors: Sugiyama Toru , Nishida Takashi , Ushijima Kimio , Sato Norio , Kataoka Akio , Imaishi Kiyohisa , Fujiyoshi Keizo , Yakushiji Michiaki 
Published: August 2010

AbstractObjective: Preoperative CT or MRI findings were compared with the results of staging laparotomy to evaluate the accuracy of CT or MRI for detecting pelvic and para‐aortic lymph‐node metastases.Methods: In evaluating CTs in 95 ovarian carcinomas, we examined plain and contrast images made in 1‐ to 1.5‐cm‐thick slices from the pubis to the xiphoid process. Lymph nodes 1.5 cm or larger were considered to be positive. MRIs of 60 uterine corpus carcinomas utilized T1‐weighted contrast, T2‐weighted, and short‐ inversion time inversion‐recovery (STIR) images.Results: CT had a sensitivity of 60.9% and a specificity of 93.1%. The positive and negative predictive values were 73.7% and 88.2%, respectively. The diagnostic accuracy of CT for detecting para‐aortic lymph‐node metastases exceeded that for pelvic node metastases. The results of MRI indicated that the T1‐weighted image and STIR image were the most accurate in identifying metastatic nodes.Conclusions: These results indicate that the most practical approach might be to search for enlarged lymph nodes by CT, and to follow‐up with MRI when CT scans are questionable.
Filtration of Human Seminal Specimens Using LeukoSorb Leukocyte‐Retention Medium
Authors: Fujii Shunsaku , Sagara Morio , Ikeda Shiro , Kagiya Akifumi , Sato Shigemi , Saito Yoshiharu 
Published: August 2010

AbstractObjective: To compare LeukoSorb filtration with Percoll centrifugation in preparing sperm.Methods: Each of 26 human seminal specimens were analyzed before and after two preparation methods.Results: In normospermic specimens (n = 11), LeukoSorb produced higher results than Percoll did with regard to mean percentage of motility (72.7% vs 59.2%), total swollen sperm in a hypo‐osmotic swelling test (84.8% vs 59.0%), (g)‐type swollen sperm (37.8% vs 23.4%) and tail abnormality (20.4% vs 10.1%). In asthenospermic specimens (n = 15), LeukoSorb produced higher results than Percoll did with regard to mean percentage of motile sperm recovery (22.2% vs 10.1%), motility (46.9% vs 23.2%), viability (75.6% vs 54.7%), total swollen sperm (84.8% vs 59.0%) and tail abnormality (42.2% vs 24.3%), although the mean percentage of head abnormality was lower with LeukoSorb than with Percoll (30.6% vs 40.2%). In pyospermic specimens (n = 10), the mean concentration of round cells after LeukoSorb decreased significantly more than with Percoll (p = 0.029). The filtration time correlated with the total sperm concentration.Conclusion: LeukoSorb filtration is useful for preparing asthenospermic and pyospermic seminal specimens.
Primary Thrombocythaemia Presenting as Postpartum Haemorrhage: A Case Report
Authors: Rashid Zainul , Hamidah N.H. , Othman Ainoon , Cheong S.K. , Fairuz A.K.A. , Adeeb Nafisah 
Published: August 2010

AbstractA young primigravida presented with postpartum haemorrhage with no apparent cause following a low forceps delivery. She was extremely pale with gross hepato‐splenomegaly. Hysterectomy was performed following three episodes of disseminated intravascular coagulation. Investigations revealed an extremely high platelet count with poor aggregatory function. A diagnosis of primary thrombocythaemia was made.
Adenomyosis in a Rudimentary Uterine Horn with Intrauterine Fetal Death: A Case Report
Authors: Numa Fumitaka , Ogata Hidenobu , Murakami Akihiro , Sase Masakatsu , Nakamura Yasuhiko , Takasugi Nobuyoshi , Kato Hiroshi 
Published: August 2010

AbstractA case of pregnancy in a rudimentary uterine horn complicated with adenomyosis resulting in intrauterine fetal death without rupture is reported. Although ultrasonographic and hysteroscopic examinations are useful, laparoscopy or laparotomy should be initiated promptly in a case where a pregnancy with a rudimentary horn is suspected.
Maternal Mortality Rate in the Obstetric Unit of University Kebangsaan Malaysia: 1981–1990
Authors: Abdullah Ashar , Mahmood Jemilah H. , Adeeb Nafisah 
Published: August 2010

AbstractThis paper analyses maternal mortality as seen in the Obstetric Unit of the University Kebangsaan Malaysia. During the 10 year study period, the maternal mortality rate was 74 / 100,000 total births. Women who were non‐booked, aged above 40 years, gradmultiparous and of India ethnicity were at the highest risk of maternal death. The commonest causes of death were hemorrhage, hypertension, embolism and sepsis. Post‐mortem examinations were performed in only 8.2% of women who died.
Mini‐Catheter Used for Bladder Drainage Following Stress‐Incontinence Surgery and the Factors Relating to Drainage Failure
Authors: Su Tsung‐Hsien , Yang Jenn‐Ming , Chen Chie‐Pein , Wei Hsiao‐Jui , Hwu Yuh‐Ming , Yang Yuh‐Cheng 
Published: August 2010

AbstractObjectives: To determine the most effective length for the mini‐catheter and to discuss factors relating to drainage failure.Methods: Prospective study of a No. 8 silastic feeding tube for bladder drainage omits the necessity of additional catheterization for measuring residual urine. This two‐part study in which 146 patients took part, first, determined the suitable length for insertions for the perforated portion and the imperforated portion; then, gauged the effectiveness of the mini‐catheter with desired proportions.Results: Drainage is better when the imperforated portion is at least 5 cm in length (p = 0.0129). The length of the perforated portion, on the other hand, has less impact of the resultant drainage provided that its length does not exceed 5 cm. Beyond this length, an obstruction often occurs. There were 2 obstructions (1.3%) induced by blood clot as well as 9 patients (5.8%) who suffered from one, or more, complications attributed to the mini‐catheter for reasons other than the length of insertion.Conclusions: When care is taken in creating a mini‐catheter of the proper proportions, it offers many advantages and very few complications to gynecologists and patients alike.
Papillary Serous Carcinoma of the Peritoneum
Authors: Chew S. , Tham K. F. , Lim F. K. , Ratnam S. S. 
Published: August 2010

AbstractObjective: To describe the clinical features of papillary serous carcinoma of the peritoneum followed by a short review of the literature.Methods: From January 1986 to May 1994, six patients with papillary serous carcinoma of the peritoneum were treated at our institution. Their presenting features, sites of disease at the time of staging laparotomy, subsequent treatment and follow‐up were reviewed.Results: The mean age was 53.3 years (range 46–59). The most common presenting features were abdominal pain, distention and the presence of ascites. Common sites of disease at the time of laparotomy were the peritoneal and subdiaphragmatic surfaces, omentum, serosa of bowel and superficial involvement of the ovaries. Patients were treated with cytoreductive surgery and platinum — based chemotherapy.Conclusion: Although experience is scant and more data is needed, for the time being, management for primary peritoneal carcinoma is as for ovarian cancer.
Investigation on Breastfeeding and Lactational Amenorrhoeic Women in Mountainous Areas of Pengxian County, Southwest China
Authors: Cui Nian , Yang Xiaofeng , Tang Guanghua , Li Minxiang , Zhang Zhiyong 
Published: August 2010

AbstractObjective: To determine the distributions of the duration of postpartum breastfeeding, amenorrhoea, and contraceptive use, a community‐based epidemiological investigation was carried out in Pengxian County, Southwest China in March 1992.Methods: 1,040 women who had given live births during the reference period were interviewed. A current‐status life table analysis was used.Results: At 1 month postpartum, 100% infants were breastfed, at 6 months 95%, and at 12 months 82%. The level of amenorrhoea at 1 month postpartum was 90%, at 3 months 74%, at 6 months 62%, and at 12 months 36%. Contraceptive use was initiated at the second month postpartum with 19% of users, at 6 months 48%, and at 12 months 91%.Conclusion: The results indicate that at population level advising women to begin using contraceptives in 3 months postpartum is fairly reasonable, and the non‐medicated IUD is recommended to be the first choice for the lactating women.
The Role of Superoxide Dismutase in the Human Ovary and Fallopian Tube*
Authors: Tamate Kenichi , Sengoku Kazuo , Ishikawa Mutsuo 
Published: August 2010

AbstractObjectives: To investigate the localization of manganese superoxide dismutase (Mn‐SOD) and copper‐zinc superoxide dismutase (Cu, Zn‐SOD) in the human ovary and fallopian tube, and to examine the role of superoxide radicals and SODs in the human ovulatory process.Methods: Using immunohistochemical methods, we studied the localization of SODs in 22 human ovaries, in 18 fallopian tubes, and in aspirated granulosa cells. We measured, by enzyme‐linked immunosorbent assay, the concentrations of SODs in follicular fluid taken from 94 IVF patients.Results: Mn‐SOD was found in granulosa, in theca and luteal cells, and in fallopian tubes. Cu, Zn‐SOD was localized in theca and luteal cells.The concentration of Cu, Zn‐SOD in follicular fluid in the high‐progesterone group (11.3 + 4.2 ng/ml) was significantly less than in the low‐progesterone group (24.5 + 19.5) (p < 0.05).Conclusion: Mn‐SOD and Cu, Zn‐SOD have different localizations and actions in human ovaries and fallopian tubes. The superoxide radical‐SOD system might play an important role in ovulation and in the luteal function of the human ovary.
The Value of Doppler Study of the Umbilical Artery in Predicting Perinatal Outcome in Pre‐Eclamptic Patients
Authors: Rocca Mohamed M. , Said Mohamed S. , Khamis Mohamed Y. , Ghanem Ibrahim A. , Karkour Tarek A. 
Published: August 2010

AbstractObjective: To test the value of routine Doppler study of the umbilical artery to predict the perinatal outcome in pre‐eclamptic patients.Methods: Fetal wellbeing was assessed in 113 women with pre‐eclampsia by weekly performed biophysical profile score (BPP) and Doppler study of the umbilical artery starting early in the third trimester till delivery.Results: The last Doppler result before delivery was normal in 82 cases and abnormal in 31 cases. The group with abnormal Doppler had significantly higher blood pressure and serum uric acid level, and significantly lower platelet count than the group with normal Doppler. The incidence of IUGR, low Apgar score at 5 minutes, perinatal deaths and operative delivery for fetal distress was significantly higher in the group with abnormal Doppler.Conclusions: The abnormal Doppler result usually precedes the appearance of abnormal BPP. The abnormal Doppler result helps to identify the fetus at risk that needs further surveillance.
Progression of Vulvar Intraepithelial Neoplasia III to Invasive Carcinoma in a Young Woman
Authors: Tan A. L. , Jones R. W. , White J. E. T. 
Published: August 2010

AbstractThis case documents the progression of untreated vulvar intraepithelial neoplasia to invasion in a young woman over a four and a half year period. Conflicting views with regards to the natural history of VIN III are discussed.
Mid‐Second Trimester Organic Acids in Severe Open Neural Tube Defects
Authors: Legge Michael 
Published: August 2010

AbstractObjective: To investigate amnioic fluid organic concentrations at 16 weeks gestation from normal fetuses and those affected by severe neural tube defects.Methods: Amniotic fluid collected from normal, anencephalic and severe open spina bifida fetuses was analysed for upto 24 organic acids by gas liquid chromatography.Results: Significant increase in organic acids similar to those observed in defects of phenylalanine and tyrosine metabolism were identified when compared with the normal fetuses. Significant differences in organic acid concentrations were also identified within the two neural tube defect groups.Conclusion: A relationship between phenylalanine and tyrosine metabolism, closure rate of the neural tube and folate metabolism is proposed.
Thyroid Hormone in Hyperemesis Gravidarum
Authors: Tareen Asal Khan , Baseer Abdul , Jaffry Hassan Fatima , Shafiq Muhammad 
Published: August 2010

AbstractObjective: Several studies have suggested that nausea and vomiting in early pregnancy are related to the levels of thyroid hormones and human chorionic gonadotropin (hCG). To ascertain this relationship, we investigated 60 pregnant women (30 subjects each with morning sickness and hyperemesis gravidarum) and compared them with 30 control subjects (by enzyme immunoassay method).Methods: Serum T3, T4 and TSH were determined in all the subjects while serum hCG was assayed in pregnant women only. Group comparison was done by applying Student's t‐test and the relationship between various parameters was evaluated by calculating coefficient of correlation, “r''.Results: Serum T4 and hCG levels were significantly increased in hyperemesis gravidarum while TSH demonstrated a significant decline in the same group. Correlation analysis showed a direct relationship between serum T4 and hCG and an inverse relationship between serum TSH and hCG in pregnancy with morning sickness.Conclusion: Our results are suggestive of the involvement of these variables in the pathogenesis of morning sickness and hyperemesis gravidarum not only because their levels were significantly altered but the extent of increase or decrease in their level correlated well with the severity of symptoms in the study subjects.
A Series of 1000 Consecutive Out‐patient Diagnostic Hysteroscopies
Authors: Cooper M. J. W. , Broadbent J. A. M. , Molnár B. G. , Richardson R. , Magos A. L. 
Published: August 2010

AbstractObjective: To review the success of out‐patient diagnostic hysteroscopy.Method: Retrospective review of 1000 consecutive out‐patient hysteroscopies.Results: Hysteroscopy was successfully performed in 96%. Cervical dilatation was required in 15.0% and local anaesthesia was administered in 31.4%; 77.3% of those requiring cervical dilatation received local anaesthesia. Intrauterine pathology was noted in 49.3%. The procedure failed in 40 (4%) patients for the following reasons: pain or anxiety in 23, cervical stenosis in 11, equipment failure in 4, and extreme uterine retroversion and inadvertent false cavity formation in one case each.Conclusion: Out‐patient diagnostic hysteroscopy is a safe, well tolerated and successful investigation procedure in the majority of patients and should be the procedure of choice for suspected intrauterine pathology.
A Case of True Hermaphroditism with Androgen Insensitivity
Authors: Takeuchi Hiroyuki , Hatta Mariko , Fukuda Masaru , Mitsuhashi Naoki , Kuwabara Yoshinori 
Published: August 2010

AbstractA phenotypically normal woman with a 46, XY karyotype was found to have a testis, an ovotestis, bilateral rudimentary uteri, and bilateral fallopian tubes. The endocrinological and pathological aspects of these findings are discussed.
Combination Treatment with Cisplatin and Schizophyllan for 7,12‐dimethylbenz(a)anthracene‐Induced Rat Ovarian Adenocarcinoma
Authors: Sugiyama Toru , Nishida Takashi , Kumagai Seisuke , Imaishi Kiyohisa , Ushijima Kimio , Kataoka Akio , Yakushiji Michiaki 
Published: August 2010

AbstractObjective: Experimental chemotherapy was conducted to investigate the combined effect of Schizophyllan (SPG) and Cisplatin (CDDP).Methods: Rats with 7,12‐dimethylbenz(a)anthracene (DMBA)‐induced ovarian adenocarcinoma received SPG and/or CDDP, were observed for the anti‐tumor effect of the drugs and were subjected to immunohistochemical study.Results: 1Tumor reduction was enhanced by the combined use of SPG and CDDP; 2The survival rate of rats given SPG alone was significantly higher than that of the control rats, and treatment with SPG combined with CDDP tended to improve the survival rate; 3Immunohistochemically, an infiltrative increase in cytotoxic T‐lymphocytes (CTL) was induced, although the development of helper T‐cells and macrophages was immunohistochemically weak at the tumor site.Conclusion: The administration of SPG enhanced the anti‐tumor effect of CDDP.
Successful Pregnancy after Intracytoplasmic Injection of a Single Testicular Spermatozoon into a Human Oocyte: A Case Report
Authors: Suganuma Nobuhiko , Asada Yoshimasa , Yamamoto Masanori , Moriwaki Takayuki , Kitagawa Takeshi , Tomoda Yutaka , Mishima Atsushi , Miyake Koji 
Published: August 2010

AbstractTesticular sperm was retrieved from a man with an epididymal obstruction, and an intracytoplasmic sperm injection was made into his wife's oocytes. In 4 mature oocytes treated, 2 fertilized eggs were obtained, and a clinical pregnancy was established with embryo transfers. Our experience demonstrated the possibility of fertilization and conception with testicular sperm.
A True Hermaphrodite with Bilateral Ovotestes: A Case Report
Authors: Sadi Al Muktafi , Toda Takayoshi , Kiyuna Masaya 
Published: August 2010

AbstractA 20‐month‐old phenotypically male child with ambiguous external genitalia came to this hospital. Chromosomal and DNA analyses revealed 46/XX karyotype and absence of SRY gene, respectively. Two gonadal masses in the labioscrotal folds histologically showed both ovarian and testicular tissues. Clinicopathological features of the patient suggested true hermaphroditism with bilateral ovotestes.
Changes in Glycoconjugate‐Lectin Binding Characteristics of in vitro and in vivo Mouse Blastocysts
Authors: Legge Michael 
Published: August 2010

AbstractObjective: To investigate the influence of embryo culture, on blastocysts cultured from the zygote stage, on blastocyst cell surface glycoconjugates as an indicator of cellular metabolism.Method: Eleven fluorescein isothiocyanate (FITC)‐labelled lectins, which recognize different oligosaccharide structures, were applied to zona pellucida intact and zona pellucida free blastocysts which had developed either in vivo or in vitro. The differential binding patterns for the zona pellucida, trophectoderm and inner cell mass were determined using fluorescence microscopy.Results: With the exception of wheat germ agglutinin (WGA) and Limax flavus agglutinin (LFA) the remaining nine lectins bound less intensely to the zona pellucida of the in vitro blastocysts compared with the in vivo blastocysts. The trophectoderm from both groups bound seven lectins with equal intensity, of the remaining four Lotus tetragonolbus agglutinin (LTA), Bandeiraea simplicifolia 4 (BSB4), Phaseolus vulgaris agglutinin P (PHA‐P) and Limulus polyphemus agglutinin (LPA) demonstrated differential binding. The inner cell mass from the two blastocyst groups bound only three lectins identically, peanut agglutinin (PNA), Bandeiraea simplicifolia 4 (BSB4) and Dolichos biflorus agglutinin (DBA).Conclusion: Oviductal glycoproteins contribute to the zona pellucida glycoprotein content during early development, however embryo culture from the zygote stage may not provide adequate substrates for normal inner cell mass glycoconjugate biosynthesis.