bluuebunny/crossref_metadata_2025_split huggingface dataset:
Examining the impact of maternal experiences of domestic violence on adolescent mental health disorders in India
Authors: Gourisankar Amritha , Ravi Preethi , Kalokhe Ameeta S. , Hall Rachel Waford , Vaidya Nilakshi , Sharma Eesha , Holla Bharath , Basu Debasish , Bharath Rose Dawn , Chakrabarti Amit , Desrivieres Sylvane , Hickman Matthew , Kartik Kamakshi , Ghattu Krishnaveni , Kalyanaraman Kumaran , Kuriyan Rebecca , Murthy Pratima , Orfanos Dimitri Papadopoulos , Purushottam Meera , Kurpad Sunita Simon , Singh Rajkumar Lenin , Singh Roshan Lourembam , Subodh Bhagyalakshmi Nanjayya , Toledano Mireille B , Benegal Vivek , Schumann Gunter , Kalyanram Kartik ,   cVEDA Consortium
Published: May 2024

AbstractBackgroundDomestic violence (DV) is experienced by one in three women in India and is linked to poor mental health outcomes. We hypothesize that maternal experiences of DV can have negative impacts on the mental health of their children. Previous studies have demonstrated this link in Western countries, however culturally specific manifestations of DV and mental health disorders and socio-cultural differences in parent-child relationships and home environments necessitate deeper understanding of the impacts of maternal experiences of DV on children in the Indian context.MethodsThis study presents a secondary analysis of data collected from a seven-center study in urban and rural India examining mental health disorders among adolescents aged 12-17 years and psychological, physical, and sexual abuse affecting their mothers. The Indian Family Violence and Control Scale (IFVCS) was used to examine experiences of DV among mothers and the Mini International Neuropsychiatric Interview–Kid (MINI-Kid) was used to examine mental health outcomes among adolescents. Multivariate analyses examined the associations between maternal DV and adolescent mental disorders.ResultsData from 2,784 adolescent-mother pairs were analyzed. In bivariate analyses, maternal experiences of physical, psychological, and sexual abuse were significantly associated with adolescent common mental disorders including anxiety and depression (p<0.05). After adjusting for adolescent gender, site, and education status in the multivariate analysis, physical, sexual, and any DV were significantly associated with adolescent anxiety disorders and common mental disorders. Physical abuse was significantly associated with adolescent depressive disorders.ConclusionsThese results suggest that exposure to maternal DV significantly impacts adolescent mental health in India and underscore the need to develop trauma-informed school programs and enhance DV prevention for women in India.
Nature-based allied health: current practice, challenges and opportunities
Authors: Stanhope Jessica , Foley Kristen , Butler Mary , Boddy Jennifer , Clanchy Kelly , George Emma , Roberts Rachel , Rothmore Paul , Salter Amy , Serocki Patricia , Thirumanickam Abirami , Weinstein Philip 
Published: May 2024

AbstractPurposeTo guide the effective use of nature-based approaches, we aimed to determine current practice, challenges and proposed solutions concerning the use of these approaches by allied health professionals (AHPs). We also investigated the signs, symptoms and conditions AHPs believe nature-based approaches may prevent and/or manage, as well as the perceived impact of the COVID-19 pandemic.Materials and methodsAllied health professionals who used or wanted to use in nature-based approaches in Australia were invited to complete an online questionnaire. Qualitative data were analysed through inductive coding and categorisation, while descriptive statistics were reported for the quantitative data.ResultsAllied health professionals indicated that exposure to nature could prevent and/or manage a range of physical and mental health, social and developmental outcomes. Perceived challenges were identified, related to the patients/clients, AHPs themselves, and external factors. Recommended solutions included increasing education for AHPs and the general public, advancing more research, implementing changes to governance, and legitimisation of nature-based approaches as part of allied health practice.ConclusionsIncreasing awareness and evidence of the use of nature-based approaches among the AHPs and across sectors – particularly with policy, education, funding, and health management– will support their legitimacy and potential benefit a range of populations.Implications for rehabilitationAllied health professionals reported that nature-based approaches may help to prevent and/or manage a range of physical, mental, social and developmental health outcomes.Challenges to implementing nature-based approaches included patient/ client, allied health professional, and external factors.To address these challenges, legitimisation of nature-based approaches is key, which may be driven predominantly by research and education.
Spatiotemporal transcriptomic maps of mouse intracerebral hemorrhage at single-cell resolution
Authors: Xiang Rong , Wang Junmin , Wang Mingyue , Chen Zhan , Tao Jin , Ding Ruoqi , Tu ZhenCheng , Wang Shaoshuai , Yang Tao , Chen Jing , Jia Zihan , Peng Qinfeng , Li Xueping , Zhang Xinru , Chen Shuai , Cheng Nannan , Zhao Mengke , Li Jiaxin , Xue Qidi , Jiang Chao , de Pablo Yolanda , Wilhelmsson Ulrika , Pekna Marcela , Pekny Milos , Mitsios Nicholas , Liu Chuanyu , Xu Xun , Fan Xiaochong , Mulder Jan , Chen Xuemei , Liu Longqi , Wang Jian 
Published: May 2024

SummaryIntracerebral hemorrhage (ICH) is a severe and widespread disease that results in high mortality and morbidity. Despite significant advances made in clinical and preclinical research, the prognosis of ICH remains poor due to an incomplete understanding of the complex pathological responses. To address this challenge, we generated single-cell resolution spatiotemporal transcriptomic maps of mouse brain 1, 3, 7, 14, and 28 days after ICH. Our analysis revealed the cellular constituents and their dynamic responses following ICH. We found changes in gene expression that are indicative of active phagocytosis and lipid processing in the lesion core and identified genes associated with brain repair at the lesion border. Persistent lipid accumulation culminated in the phenotypic transition of macrophages into foam cells. Furthermore, our results demonstrate that ICH could stimulate neural stem cells in the subventricular zone, thereby enhancing neurogenesis in this niche. This study provides a spatiotemporal molecular atlas of mouse ICH that advances the understanding of both local and global responses of brain cells to ICH and offers a valuable resource that can aid the development of novel therapies for this devastating condition.
Potassium homeostasis during disease progression of Alzheimer’s Disease
Authors: Samokhina Evgeniia , Mangat Armaan , Malladi Chandra S. , Gyengesi Erika , Morley John W. , Buskila Yossi 
Published: May 2024

AbstractAlzheimer’s disease (AD) is an age-dependent neurodegenerative disorder characterized by neuronal loss leading to dementia and ultimately death. Whilst the loss of neurons is central to the disease, it is becoming clear that glia, specifically astrocytes, contribute to the onset and progression of neurodegeneration. Astrocytic role in retaining ion homeostasis in the extracellular milieu is fundamental for multiple brain functions, including synaptic plasticity and neuronal excitability, which are compromised during AD and affect neuronal signalling. In this study, we have measured the astrocytic K+clearance rate in the hippocampus and somatosensory cortex of a mouse model for AD during disease progression. Our results establish that astrocytic [K+]oclearance in the hippocampus is reduced in symptomatic 5xFAD mice, and this decrease is region-specific. The decrease in the [K+]oclearance rate correlated with a significant reduction in the expression and conductivity of Kir4.1 channels and a decline in the number of primary connected astrocytes. Moreover, astrocytes in the hippocampus of symptomatic 5xFAD mice demonstrated increased reactivity which was accompanied by an increased excitability and altered spiking profile of nearby neurons. These findings indicate that the supportive function astrocytes typically provide to nearby neurons is diminished during disease progression, which affects the neuronal circuit signalling in this area and provides a potential explanation for the increased vulnerability of neurons in AD.
The Effects of Locus Coeruleus Optogenetic Stimulation on Global Spatiotemporal Patterns in Rats
Authors: Anumba Nmachi , Kelberman Michael A. , Pan Wenju , Marriott Alexia , Zhang Xiaodi , Xu Nan , Weinshenker David , Keilholz Shella 
Published: May 2024

ABSTRACTWhole-brain intrinsic activity as detected by resting-state fMRI can be summarized by three primary spatiotemporal patterns. These patterns have been shown to change with different brain states, especially arousal. The noradrenergic locus coeruleus (LC) is a key node in arousal circuits and has extensive projections throughout the brain, giving it neuromodulatory influence over the coordinated activity of structurally separated regions. In this study, we used optogenetic-fMRI in rats to investigate the impact of LC stimulation on the global signal and three primary spatiotemporal patterns. We report small, spatially specific changes in global signal distribution as a result of tonic LC stimulation, as well as regional changes in spatiotemporal patterns of activity at 5 Hz tonic and 15 Hz phasic stimulation. We also found that LC stimulation had little to no effect on the spatiotemporal patterns detected by complex principal component analysis. These results show that the effects of LC activity on the BOLD signal in rats may be small and regionally concentrated, as opposed to widespread and globally acting.
Top-down and bottom-up interactions rely on nested brain oscillations
Authors: Trajkovic Jelena , Veniero Domenica , Hanslmayr Simon , Palva Satu , Cruz Gabriela , Romei Vincenzo , Thut Gregor 
Published: May 2024

AbstractAdaptive visual processing is enabled through the dynamic interplay between top-down and bottom-up (feedback/feedforward) information exchange, presumably propagated through brain oscillations. Here we causally tested for the oscillatory mechanisms governing this interaction in the visual system. Using concurrent TMS-EEG, we emulated top-down signals by a single TMS-pulse over the Frontal Eye Field (right-FEF), while manipulating the strength of sensory input through the presentation of moving concentric gratings (compared to a control-TMS site). FEF-TMS without sensory input led to a top-down controlled occipital phase-realignment, alongside higher fronto-occipital phase-connectivity, in the alpha/beta-band. Sensory input in the absence of FEF-TMS increased occipital gamma activity. Crucially, testing the interaction between top-down and bottom-up processes (FEF-TMS during sensory input) revealed an increased nesting of the bottom-up gamma activity in the alpha/beta-band cycles. This establishes a causal link between phase-to-power coupling and top-down modulation of feedforward signals, providing novel mechanistic insights into how attention interacts with sensory input at the neural level, shaping rhythmic sampling.
FADS and semi-rational design modified T7 RNA polymerase reduced dsRNA production, with lower terminal transferase and RDRP activities
Authors: Tang Qiongwei , Zhu Sisi , Hu Nannan , Yin Sainan , Yang Yuhong , Teng Yigang , Song Dongliang , Liu Xiang 
Published: May 2024

AbstractT7 RNA polymerase (T7 RNAP) is the preferred tool forin vitrotranscription (IVT), it synthesizes mRNA while accompanied by the generation of dsRNA by-products. This undesirable dsRNA triggers immune stress responses, compromises therapeutic efficacy, and raises safety concerns. To evolve T7 RNAP for reduced dsRNA, we pursued two complementary strategies. Firstly, the FADS (fluorescence-activated droplet sorting) based on molecular beacons was used to screen random libraries with diversity exceeding 105. Secondly, we constructed several single-site saturated libraries to facilitate the transition of T7 RNAP from the initiation to the elongation conformation. These libraries were screened using the traditional microplate-based dual-probe screening technique. Both approaches identified two dominant variants: Mut1 (V214A) and Mut7 (F162S/A247T) from FADS, Mut11 (K180E) and Mut14 (A70Q) from saturated libraries. Furthermore, the combinatorial mutant Mut17 (A70Q/F162S/K180E), generated via DNA shuffling, exhibited significantly reduced dsRNA production compared to the wild-type under various conditions, ranging from 0.18% to 1.80%, with a minimum value of 0.5 pg/μg. Cell experiments confirmed that variants generated capped-mRNA with similar quality and quantity to the wild-type, while significantly reducing immune stress response in cells. These results indicate the compatibility and broad potential applications of these mutations. We then observed a close correlation between the production of dsRNA and the activities of T7 RNAP in terminal transferase and RDRP. Particularly, the terminal transferase activity appears to play a critical role in dsRNA generation. These findings align with the mechanism of dsRNA formation during IVT and provide new screening criteria for further evolution of T7 RNAP.
Microbiomes of three coral species in the Mexican Caribbean and their shifts associated with the Stony Coral Tissue Loss Disease
Authors: Arriaga-Piñón Zita P. , Aguayo-Leyva J. Eduardo , Álvarez-Filip Lorenzo , Banaszak Anastazia T. , Macedo Ma. Leopoldina Aguirre , Paz-García David A. , García-Maldonado José Q. 
Published: May 2024

AbstractStony Coral Tissue Loss Disease (SCTLD) has caused widespread coral mortality in the Caribbean Region. However, how the disease presence alters the microbiome community, their structure, composition, and metabolic functionality is still poorly understood. In this study, we characterized the microbial communities of the tissues of apparently healthy and diseased SCTLD colonies of the speciesSiderastrea siderea,Orbicella faveolata, andMontastraea cavernosato explore putative changes related to the presence of SCTLD.Gammaproteobacteria,Alphaproteobacteria, andBacteroidiawere the best represented classes in the healthy tissues of all coral species, and alpha diversity did not show significant differences among the species. The microbial community structure between coral species was significantly different (PERMANOVA: F=3.46, p=0.001), and enriched genera were detected for each species:VibrioandPhotobacteriuminS. siderea,Spirochaeta2andMarivivensinO. faveolataand SAR202_clade andNitrospirainM. cavernosa. Evidence of SCTLD in the microbial communities was more substantial inS. siderea, where differences in alpha diversity, beta diversity, and functional profiles were observed. InO. faveolata, differences were detected only in the community structure, whileM. cavernosasamples showed no significant difference. Several microbial groups were found to have enriched abundances in tissue from SCTLD lesions fromS. sidereaandO. faveolata, but no dominant bacterial group was detected. Our results contribute to understanding microbial diversity associated with three scleractinian coral species and the shifts in their microbiomes associated with SCTLD in the Mexican Caribbean.
Distinct release properties of glutamate/GABA co-transmission serve as a frequency-dependent filtering of supramammillary inputs
Authors: Hirai Himawari , Konno Kohtarou , Yamasaki Miwako , Watanabe Masahiko , Sakaba Takeshi , Hashimotodani Yuki 
Published: May 2024

AbstractGlutamate and GABA co-transmitting neurons exist in several brain regions; however, the mechanism by which these two neurotransmitters are co-released from the same synaptic terminals remains unclear. Here, we show that the supramammillary nucleus (SuM) to dentate granule cell synapses, which co-release glutamate and GABA, exhibit differences between glutamate and GABA release properties in paired-pulse ratio, Ca2+-sensitivity, presynaptic receptor modulation, and Ca2+channel-vesicle coupling configuration. Moreover, uniquantal synaptic responses show independent glutamatergic and GABAergic responses. Morphological analysis reveals that most SuM terminals form distinct glutamatergic and GABAergic synapses in proximity, each characterized by GluN1 and GABAAα1 labeling, respectively. Notably, glutamate/GABA co-transmission exhibits distinct short-term plasticities, with frequency-dependent depression of glutamate and frequency-independent stable depression of GABA. Our findings suggest that glutamate and GABA are co-released from different synaptic vesicles within the SuM terminals, and reveal that distinct transmission modes of glutamate/GABA co-release serve as frequency-dependent filters of SuM inputs.
A single septin from a polyextremotolerant yeast recapitulates many canonical functions of septin hetero-oligomers
Authors: Hamilton Grace E , Wadkovsky Katherine N , Gladfelter Amy S 
Published: May 2024

AbstractMorphological complexity and plasticity are hallmarks of polyextremotolerant fungi. Septins are conserved cytoskeletal proteins and key contributors to cell polarity and morphogenesis. They sense membrane curvature, coordinate cell division, and influence diffusion at the plasma membrane. Four septins homologs are conserved from yeasts to humans, the two systems in which septins have been studied most extensively. But there is also a fifth family of septin proteins that remain biochemically mysterious. Members of this family, known as Group 5 septins, appear in the genomes of filamentous fungi, and thus have been understudied due to their absence from ascomycete yeasts.Knufia petricolais an emerging model polyextremotolerant black fungus that can serve as a model system for understudied Group 5 septins. We have recombinantly expressed and biochemically characterizedKpAspE, a Group 5 septin fromK. petricola, demonstrating that this septin––by itselfin vitro–– recapitulates many of the functions of canonical septin hetero-octamers.KpAspE is an active GTPase that forms diverse homo-oligomers, senses membrane curvature, and interacts with the terminal subunit of canonical septin hetero-octamers. These findings raise the possibility that Group 5 septins govern the higher order structures formed by canonical septins, which inK. petricolacells form extended filaments. These findings provide insight into how septin hetero-oligomers evolved from ancient homomers and raise the possibility that Group 5 septins govern the higher order structures formed by canonical septins.Significance StatementSeptins are understudied cytoskeletal proteins. Here, we biochemically characterizedKpAspE, a novel Group 5 septin from a polyextremotolerant black fungus.KpAspE in isolation recapitulates many properties of canonical septin hetero-octamersin vitroand interacts with the Cdc11, the terminal subunit of those octamers.These findings provide insight into how ancient septins may have evolved and diversified from homopolymers and suggest that many of the septin functions were present in the ancestral protein.
Design principles for engineering bacteria to maximise chemical production from batch cultures
Authors: Mannan Ahmad A. , Darlington Alexander P.S. , Tanaka Reiko J. , Bates Declan G. 
Published: May 2024

AbstractBacteria can be engineered to manufacture chemicals, but it is unclear how to optimally engineer a single cell to maximise production performance from batch cultures. Moreover, the performance of engineered production pathways is affected by competition for the host’s native resources. Here, using a “host-aware” computational framework which captures competition for both metabolic and gene expression resources, we uncover design principles for engineering the expression of host and production enzymes in a cell to maximise volumetric productivity and yield from batch cultures. Our results suggest that selecting strains in the lab for maximum growth and product synthesis can achieve close to maximum culture productivity and yield, but the growth-synthesis trade-off fundamentally limits production performance. We show that engineering genetic circuits to switch cells to a high synthesis-low growth state after first growing to a large population can further improve performance. By analysing different circuit topologies, we show that optimal performance is achieved by circuits that inhibit host metabolism to redirect it to product synthesis. Our results should facilitate construction of microbial cell factories with high and efficient production capabilities.
A Scaffolding Element Rewires Local 3D Chromatin Architecture During Differentiation
Authors: Jerković Ivana , Di Stefano Marco , Reboul Hadrien , Szalay Michael F , Normanno Davide , Papadopoulos Giorgio L , Bantignies Frederic , Cavalli Giacomo 
Published: May 2024

1AbstractUpon differentiation chromatin rewires to reflect its new cellular identity and function. While it is widely known that this process involves cooperative changes in transcription, chromatin composition and 3D conformation, it is unclear what exactly drives these changes and how they influence one another. Here we used ESC-to-NPC differentiation to study rewiring at a 3 Mb large neuronalZfp608locus. During this process, this large chromatin domain splits in half right at theZfp608promoter, local chromatin gets littered with activating marks, compacts in 3D space andZfp608abounds in transcription. We investigated thecisandtranselements using capture Hi-C (cHi-C), extensive biophysical modelling, and 3-colour 3D-FISH with technical and analytical breakthroughs and found that transcription abundance modulates the contacts in the region as well as the insulation at the domain split. Furthermore, we found a genetic element we named scaffolding element, with a dual enhancer and architectural function that is essential for chromatin rewiring and loop formation at the NPC stage. The loss of this element disrupts the formation of all local NPC-loops irrespective if they are anchored in this element or not, highlighting the hierarchical relationship between elements that act as loop anchors. Furthermore, we uncovered that the scaffolding function, although driven by multiple mechanisms, can form loops independent of loop-extrusion and that other molecular attractions were necessary to form NPC-specific contacts in the region. Together, these results demonstrate that a hierarchy of genetic elements incisallows successful rewiring during differentiation and that multipletransacting elements contribute to make this rewiring efficient.