bluuebunny/crossref_metadata_2025_split huggingface dataset:
High‐dose chemotherapy in relapsed or refractory Hodgkin lymphoma patients: a reappraisal of prognostic factors
Authors: Cocorocchio E , Peccatori F , Vanazzi A , Piperno G , Calabrese L , Botteri E , Travaini L , Preda L , Martinelli G 
Published: April 2012

AbstractHigh‐dose chemotherapy (HDCT) has a consolidated role in the treatment of patients with refractory or relapsed Hodgkin lymphoma (HL). We report clinical results of 97 HL patients who underwent HDCT for refractory (62 patients) or relapsed (35 patients) diseases in Istituto Europeo di Oncologia, from 1995 to 2009. Treatment included high‐dose carmustine, etoposide, cytarabine and melphalan in 84 patients and high‐dose idarubicin and melphalan in 13 patients with subsequent peripheral hemopoietic stem cells transplant. Outcomes were evaluated in terms of progression‐free survival (PFS) and overall survival (OS). In order to identify prognostic factors for outcome, a multivariate analysis for age, sex, disease status (refractory/relapsed), disease stage, B symptoms, presence of extranodal involvement, bulky disease, elevated lactate dehydrogenase, number of previous chemotherapy lines, remission status before transplant, 18F‐fluoro‐deoxy‐d‐glucose positron emission tomography (18FDG‐PET) status before and after transplant was done. A clinical response was achieved in 91% of patients, with complete remissions in 76/97 patients. With a median follow‐up of 45 months (range 1–164 months), 5‐year PFS and OS were 64% and 71%, respectively. Remission status after induction therapy, 18F‐fluoro‐deoxy‐d‐glucose positron emission tomography status before and after transplant were the most important prognostic factors for PFS and OS in univariate or multivariate analyses. HDCT is able to induce a high remission rate and a prolonged PFS in more than 50% of the patients with refractory and relapsed HL. Copyright © 2012 John Wiley & Sons, Ltd.
The 19S proteasome subunit Rpn7 stabilizes DNA damage foci upon genotoxic insult
Authors: Tsolou Avgi , Nelson Glyn , Trachana Varvara , Chondrogianni Niki , Saretzki Gabriele , von Zglinicki Thomas , Gonos Efstathios S. 
Published: April 2012

AbstractThe DNA damage response (DDR) orchestrates the recruitment of repair proteins at sites of damage and arrests cell‐cycle progression until completion of repair. Upon irreparable damage, DNA damage foci persist (long‐lived foci) and this is believed to induce cellular senescence. The resolution of DNA damage foci has previously been shown to depend on proteasomal degradation and various proteasome subunits have been implicated in the DDR. In this study, we aimed to analyze the possible distinct roles of individual proteasome subunits in the DDR. We show that specific 19S subunits respond to DNA damage by increased protein levels and nuclear translocation. Importantly, two 19S subunits, Rpn7 and Rpn11, colocalize with DNA damage foci over their whole lifespan. Although silencing of Rpn11 does not affect foci stability and lifespan, silencing of Rpn7 promotes faster resolution of DNA damage foci following genotoxic insult. For the first time, we provide evidence that Rpn7 silencing specifically decreases the frequencies of long‐lived DNA damage foci without, however, affecting the repair rate of short‐lived foci. Therefore, we propose that interaction of Rpn7 with DDR foci in situ mediates the protection of DNA damage foci from premature resolution. We suggest that this interaction is involved in enabling cellular senescence following genotoxic insult. 2012 IUBMB IUBMB Life, 2012
Cytoplasmatic compartmentalization by Bcr‐Abl promotes TET2 loss‐of‐function in chronic myeloid leukemia
Authors: Mancini Manuela , Veljkovic Nevena , Leo Elisa , Aluigi Michela , Borsi Enrica , Galloni Chiara , Iacobucci Ilaria , Barbieri Enza , Santucci Maria Alessandra 
Published: April 2012

AbstractThe loss‐of‐function of ten–eleven‐translocation (TET) 2, a Fe2+‐oxoglutarate‐dependent dioxygenase catalyzing 5 methyl cytosine (5mC) conversion into 5‐hydroxymethylcytosine (5hmC), contributes to the hematopoietic transformation in vivo. The aim of our study was to elucidate its role in the phenotype of chronic myeloid leukemia (CML), a myeloproliferative disease caused by the Bcr‐Abl rearranged gene. We first confirmed TET2 interaction with the Bcr‐Abl protein predicted by a Fourier‐based bioinformatic method. Such interaction led to TET2 cytoplasmatic compartmentalization in a complex tethered by the fusion protein tyrosine kinase (TK) and encompassing the Forkhead box O3a (FoxO3a) transcription factor. We then focused the impact of TET2 loss‐of‐function on epigenetic transcriptional regulation of Bcl2‐interacting mediator (BIM), a pro‐apoptotic protein transcriptionally regulated by FoxO3a. BIM downregulation is a critical component of CML progenitor extended survival and is also involved in the disease resistance to imatinib (IM). Here we reported that TET2 release from Bcr‐Abl protein following TK inhibition in response to IM triggers a chain of events including TET2 nuclear translocation, re‐activation of its enzymatic function at 5mC and recruitment at the BIM promoter followed by BIM transcriptional induction. 5hmC increment following TET2 re‐activation was associated with the reduction of histone H3 tri‐methylation at lysine 9 (H3K9me3), which may contribute with DNA de‐methylation reported elsewhere to recast a permissive epigenetic “landscape” for FoxO3a transcriptional activity. J. Cell. Biochem. 113: 2765–2774, 2012. © 2012 Wiley Periodicals, Inc.
Synthesis, Characterization, Catalytic and Antibacterial Activity of Two Series of Dinuclear Ruthenium Sulphoxide Complexes Using 5,5¢‐Methylenebis(pyridine) as Bis‐chelating Bridging Ligand
Authors: Shukla Satyendra N. , Gaur Pratiksha , Prasad Mamta , Prasad Mahender , Mehrotra Ripul , Mathews Sheela 
Published: April 2012

AbstractThe reaction of a new heterocyclic bidentate N containing spacer, (ligand) 5,5′‐methylenebis(pyridine) with ruthenium sulphoxide precursors resulted, dinuclear complexes. We herein report three formulations; [{cis,fac‐RuCl2(so)3}2(μ‐mbp)].3so; [{trans,mer‐RuCl2(so)32}2(μ‐mbp)].3so and [{trans‐RuCl4(so)}2(μ‐mbp)]2−[X]2+; where so = dimethyl‐sulfoxide/tetramethylenesulfoxide; mbp = 5,5′‐methylenebis(pyridine) and [X]+ = [(dmso)2H]+, Na+ or [(tmso)H]+. These complexes were characterized on the basis of elemental analyses, molar conductance measurement, magnetic susceptibility, FT‐IR, 1H‐NMR, 13C{1H}‐NMR, electronic spectroscopy and FAB‐Mass spectrometry. Catalytic activity of these complexes has been investigated in hydrolysis of benzonitrile. All the complexes exhibit good antibacterial activity against gram‐negative bacteria Escherichia coli in comparison to Chloramphenicol.
Profile of Parkia speciosa Hassk Metabolites Extracted with SFE using FTIR‐PCA Method
Authors: Rahman Nik Norulaini Nik Abd. , Zhari Salman , Sarker Md. Zaidul Islam , Ferdosh Sahena , Yunus Mohd Azizi Che , Kadir Mohd Omar Abd 
Published: April 2012

AbstractA rapid identification, classification and discrimination tool, using Fourier Transform Infrared (FTIR) spectroscopy combined with Principal Component Analysis (PCA), was developed and applied to determine the profile of the Supercritical Fluid Extraction (SFE) of Parkia speciosa seeds under various temperature and pressure conditions (313, 323, 333, 343, 353 and 363 K and 20.68, 27.58, 34.47, 41.37, 48.26, and 55.16 MPa). The separation and identification of the compounds was carried out by Gas Chromatography coupled with Time of Flight Mass Spectrometry (GC/TOF‐MS). This technique has made it possible to detect the variability obtained under different SFE conditions and the separation of different chemical compounds in P. speciosa seeds. The FTIR‐PCA results were verified by GC/TOF‐MS, and the FTIR‐PCA method successfully identified the unsaturated carboxylic acids with the highest percentage area under the different conditions.
Trace Determination of Rhodamine B and Rhodamine 6G Dyes in Aqueous Samples by Solid‐phase Extraction and High‐performance Liquid Chromatography Coupled with Fluorescence Detection
Authors: Chiang Tsung‐Ling , Wang Yu‐Chen , Ding Wang‐Hsien 
Published: April 2012

AbstractA simple and reliable method was developed to detect two basic synthetic dyes, rhodamine B (RB) and rhodamine 6G (R6G), in wastewater and surface water samples by high performance liquid chromatography with fluorescence detection (HPLC‐FLD). These dyes have been reported to be both mutagenic and carcinogenic in various organisms. The contents of these two dyes in water samples were extracted by Oasis HLB solid‐phase extraction (HLB‐SPE), and were then determined by an isocratic HPLC using an Atlantis® T3‐C18 column. Water samples at various pH conditions and the compositions of eluents for SPE were evaluated. The results indicate that the proposed method is precise and sensitive in analyzing these two basic synthetic dyes, and the limits of quantitation were 1.5 ng/L for RB and 0.3 ng/L for R6G in 100 mL of water samples. The recovery of analytes in spiked surface water and municipal wastewater treatment plant (WWTP) effluent samples ranged from 61 to 90% with the precision (RSD) ranging from 2 to 12%. The concentrations of analytes were detected in various water samples ranging from 0.7 to 81 ng/L.
DNA Binding and Cleavage Activity of cis‐Cobalt Aromatic Oxime Complexes and Its Pyridine/imidazole Adducts
Authors: Surendrababu M. S. , Reddy K. Hussain 
Published: April 2012

Abstractcis‐Cobalt complexes with salicycaldoxime(SAO), (Z)‐1‐(2‐hydroxyphenyl)ethanonoxime (HEO), (Z)‐1‐(2,5‐dihydroxyphenyl)ethanonoxime (DEO), (Z)‐1‐(2,5‐dihydroxyphenyl)(phenyl)methanonoxime (DPO) and their adducts with pyridine (Py) and imidazole (Im) were synthesized and characterized by elemental analysis, magnetic susceptibility, UV‐Vis and IR spectra. The electrochemical studies were carried by cyclic voltammeter, the peak potential separation and formal potential of complexes were independent of sweep rate or scan rate (ν) indicating a quasi reversible one‐electron redox process. Absorption studies and thermal denature studies revealed that each of these octahedral complexes is an avid binder of calf thymus DNA. The apparent binding constants for mixed ligand complexes are in order of ∼103‐103 M−1. Based on the data obtained in the DNA binding studies a partial intercalative mode of binding is suggested for these complexes. The nucleolytic cleavage activity of parent complexes and their pyridine adduct were carried out on double stranded pBR322 circular plasmid DNA by using a gel electrophoresis experiment in the presence and absence of oxidant (H2O2). All the metal complexes show enhanced cleavage activity in presence of oxidant. The hydrolytic cleavage of DNA of Co(DEO)2 and Co(DPO)2 is evidenced from the control experiments showing discernable cleavage inhibition in the presence of the hydroxyl radical inhibitor DMSO and EDTA.
Some Reactions of gem‐Dibromocyclopropanes and Metal Carbonyls
Authors: Lin Shaw‐Tao , Lee Chuan‐Chen , Ding Mei‐Fang , Liang David W. , Jeng An‐Ting 
Published: April 2012

AbstractA series of gem‐dibromocyclopropanes were treated with various metal complexes. Among the metal complexes, Ru(CO)2(PPh3)3, Ru(CO)3(PPh3)2, and Mo(CO)6 were able to remove a bromine atom from 1,1‐dibromo‐2‐phenylcyclopropanes (1) to yield a series of corresponding of 1‐bromo‐2‐phenylcyclopropanes (2). Upon the treatment of 1 with Cr(CO)6 in DMSO, a series of allenes were obtained in good yields. The correlation between the rate of formation of allenes and the substituents on the benzene gives a negative coefficient which suggests the dibromocyclopropanes possesses as an electrophile toward to Cr(CO)6. In the presence of Cr(CO)6, gem‐dibromobicyclo[n,1,0]alkanes (4) in DMF or DMSO solution underwent the cleavage of carbon‐bromine bond followed by ring‐expansion and coupling reaction to form bicycloalkenes 7.
Catalyst Free Indirect Friedländer Synthesis of Substituted Quinolines from Alcohols in PEG‐400
Authors: Wu Fang‐Wen , Hou Rei‐Sheu , Wang Huey‐Min , Kang Iou‐Jiun , Chen Ling‐Ching 
Published: April 2012

AbstractThe synthesis of substituted quinolines can be easily and greenly accomplished by the direct reaction between the corresponding aminoalcohol and ketone using PEG‐400 as reaction medium in the presence of a base, without any transition‐metal catalyst.
Electrochemical Behaviors of Baicalin at an Electrochemically Activated Glassy Carbon Electrode and Its Determination in Human Blood Serum
Authors: Wang Fei , Lv Ming‐Xiu , Lu Kui , Gao Lin , Liu Jian 
Published: April 2012

AbstractElectrochemical behavior of baicalin (BA) at an electrochemically activated glassy carbon electrode (GCE) had been investigated in Britton‐Robinson (B‐R) buffer solution (pH = 2.87) by cyclic voltammetry (CV) and square wave voltammetry (SWV). The experimental results suggested that the electrode exhibited an electrocatalytic activity toward the redox of BA. The electron transfer coefficient (α) and the standard rate constant (ks) of BA at the electrochemically activated glassy carbon electrode were calculated. The reaction mechanism was proposed and discussed in this work. Under the selected conditions, the reduction peak current was linearly dependent on the concentration of BA in the range of 5.0 × 10−8 to 3.0 × 10−6 mol L−1 (r = 0.9990), with a detection limit of 2.0 × 10−8 mol L−1. The relative standard deviation (R.S.D.) for five times successful determination of 1.0 × 10−6 mol L−1 baicalin were 2.9%. The proposed method was also successfully applied for the determination of BA in human blood serum.
Two New 4‐Hydroxyisoflavanes from the Root of Codonopsis cordifolioidea and Their Anti‐virus Activities
Authors: Gao Xuemei , Mu Huaixue , Li Xuesen , Yang Guangyu , Li Ganpeng , Hu Qiufen 
Published: April 2012

AbstractTwo new 4‐hydroxyisoflavanes, cordifoliflavanes A and B (1 and 2), were isolated from the roots of Codonopsis cordifolioidea. Their structures were elucidated by spectroscopic methods, including extensive 1D‐ and 2D‐NMR techniques. Compounds 1 and 2 were tested for their anti‐HIV‐1 activities and anti‐tobacco mosaic virus activities. The results showed that compounds 1 and 2 have modest anti‐HIV‐1 activities and anti‐tobacco mosaic virus activities, respectively.
Evaluation of VOC emissions control and opacity removal using a biological sequential treatment system for forest products applications
Authors: Karre Anand , Jones Kim , Boswell Jim , Paca Jan 
Published: April 2012

AbstractBACKGROUND: The emissions at the Stimson Lumber Company plant, Gaston, Oregon, USA, were treated with a pilot scale biological system to remove low concentrations of volatile organic compounds (VOCs) (principally pinene, formaldehyde and methanol) emitted from the wet process hard board manufacturing section of the plant. The objective was to demonstrate the effectiveness of the system for optimal removal of VOCs and reduction of opacity (blue haze) of emissions from the wet press.RESULTS: Analysis of the air samples collected from the bio‐treatment system showed a removal efficiency of 90% for total VOCs. The optimization experiments demonstrated that the VOC removal rates improved as the gas flow rate and VOC loading rates increased, and at higher recycle water flow rates, which suggested additional treatment capacity for the biofiltration unit when operated at 40 s empty bed retention time. The opacity of emissions measured using both the US EPA Method‐9 and Wager 6500 opacity meter showed similar trends.CONCLUSIONS: The biological system demonstrated efficient removal of mixtures of forest products VOC emissions with elimination capacities in the range 8–10 gVOC m−3 h−1. The opacity reduction and VOC removal data were correlated demonstrating that inline opacity measurements may be useful for biosystem performance monitoring and troubleshooting. Copyright © 2012 Society of Chemical Industry
Application of the aquatic fungus Phoma sp. (DSM22425) in bioreactors for the treatment of textile dye model effluents
Authors: Junghanns Charles , Neumann Jan Felix , Schlosser Dietmar 
Published: April 2012

AbstractBACKGROUND: Textile dyes are hardly removed from effluents by conventional wastewater treatment but can be degraded by a number of physicochemical processes, which nevertheless have specific limitations. The development of bioremediation processes may provide a viable alternative.RESULTS: Resting cells of three aquatic fungal strains, Alternaria sp. (Tt‐S1), Coniothyrium sp. (Kl‐S5) and Phoma sp. (DSM22425), were evaluated for their ability to decolorize model effluents of different dye application classes. Phoma sp., which decolorized all four applied model wastewaters (MWW), was immobilized on a polyester‐based fleece material originally designed for use as cooker‐hood filters, and applied in internal loop airlift and bubble column reactors in repeated batch mode under sterile and non‐sterile conditions. Depending on the applied MWW and bioreactor setup, a decolorization of more than 90% was achieved in three consecutive treatment cycles and total operating time of 16 days. In an upscaled reactor (10 L), Phoma sp. decolorized MWW containing acid dyes by 61%. Chemical oxygen demand of the MWW was reduced by 36%.CONCLUSION: The results presented underline the potential of aquatic fungi for the development of textile dyeing effluent treatment processes. Copyright © 2012 Society of Chemical Industry
Acyclic 4‐Choosability of Planar Graphs with No 4‐ and 5‐Cycles
Authors: Borodin Oleg V. , Ivanova Anna O. 
Published: April 2012

AbstractEvery planar graph is known to be acyclically 7‐choosable and is conjectured to be acyclically 5‐choosable (O. V. Borodin, D. G. Fon‐Der‐Flaass, A. V. Kostochka, E. Sopena, J Graph Theory 40 (2002), 83–90). This conjecture if proved would imply both Borodin's (Discrete Math 25 (1979), 211–236) acyclic 5‐color theorem and Thomassen's (J Combin Theory Ser B 62 (1994), 180–181) 5‐choosability theorem. However, as yet it has been verified only for several restricted classes of graphs. Some sufficient conditions are also obtained for a planar graph to be acyclically 4‐ and 3‐choosable. In particular, the acyclic 4‐choosability was proved for the following planar graphs: without 3‐, 4‐, and 5‐cycles (M. Montassier, P. Ochem, and A. Raspaud, J Graph Theory 51 (2006), 281–300), without 4‐, 5‐, and 6‐cycles, or without 4‐, 5‐, and 7‐cycles, or without 4‐, 5‐, and intersecting 3‐cycles (M. Montassier, A. Raspaud, W. Wang, Topics Discrete Math (2006), 473–491), and neither 4‐ and 5‐cycles nor 8‐cycles having a triangular chord (M. Chen and A. Raspaud, Discrete Math. 310(15–16) (2010), 2113–2118). The purpose of this paper is to strengthen these results by proving that each planar graph without 4‐ and 5‐cycles is acyclically 4‐choosable.
Impact of hyponatremia and the syndrome of inappropriate antidiuresis on mortality in elderly patients with aspiration pneumonia
Authors: Miyashita Jun , Shimada Toshihiko , Hunter Alan J. , Kamiya Toru 
Published: April 2012

AbstractBACKGROUND:Hyponatremia is associated with pneumonia, and aspiration pneumonia is common in the elderly, however, there has been no study of hyponatremia in this population.OBJECTIVE:To determine the impact of hyponatremia on mortality in elderly patients with aspiration pneumonia, while focusing on the comparison between those with and without the syndrome of inappropriate antidiuresis (SIAD).DESIGN:Retrospective review of existing database and medical records.SETTING:A community teaching hospital in Japan.PATIENTS:Two hundred and twenty‐one elderly patients hospitalized with aspiration pneumonia.MEASUREMENTS:Multivariate logistical regression models were used to compare 30‐day and in‐hospital mortality, in patients with hyponatremia of various severities and etiologies, with that in patients with normal serum sodium concentrations.RESULTS:Sixty‐five (29%) of 221 patients had hyponatremia. Of these 62 (95%) had hypotonic hyponatremia, which were further assessed as having hypovolemic (39 [63%]), hypervolemic (3 [5%]), and euvolemic (20 [32%]) hyponatremia. Of the 20 euvolemic patients, 14 (70%) had SIAD. Both moderate and severe hypotonic hyponatremia were significantly associated with increased in‐hospital mortality (odds ratio [OR] 6.05, 95% confidence interval [CI] 1.46–25.0, and OR 5.65, 95% CI 1.14–28.1, respectively). Hyponatremia due to SIAD was significantly associated with both increased 30‐day mortality (OR 7.40, 95% CI 1.73–31.7) and increased in‐hospital mortality (OR 22.3, 95% CI 4.26–117). In contrast, hypovolemic hyponatremia was not significantly associated with increased mortality.CONCLUSIONS:Hyponatremia due to SIAD was strongly associated with increased mortality in elderly patients with aspiration pneumonia, whereas hypovolemic hyponatremia was not associated with increased mortality. Journal of Hospital Medicine 2012;. © 2012 Society of Hospital Medicine
Predictors of medication adherence postdischarge: The impact of patient age, insurance status, and prior adherence
Authors: Cohen Marya J. , Shaykevich Shimon , Cawthon Courtney , Kripalani Sunil , Paasche‐Orlow Michael K. , Schnipper Jeffrey L. 
Published: April 2012

AbstractBACKGROUND:Optimizing postdischarge medication adherence is a target for avoiding adverse events. Nevertheless, few studies have focused on predictors of postdischarge medication adherence.METHODS:The Pharmacist Intervention for Low Literacy in Cardiovascular Disease (PILL‐CVD) study used counseling and follow‐up to improve postdischarge medication safety. In this secondary data analysis, we analyzed predictors of self‐reported medication adherence after discharge. Based on an interview at 30‐days postdischarge, an adherence score was calculated as the mean adherence in the previous week of all regularly scheduled medications. Multivariable linear regression was used to determine the independent predictors of postdischarge adherence.RESULTS:The mean age of the 646 included patients was 61.2 years, and they were prescribed an average of 8 daily medications. The mean postdischarge adherence score was 95% (standard deviation [SD] = 10.2%). For every 10‐year increase in age, there was a 1% absolute increase in postdischarge adherence (95% confidence interval [CI] 0.4% to 2.0%). Compared to patients with private insurance, patients with Medicaid were 4.5% less adherent (95% CI −7.6% to −1.4%). For every 1‐point increase in baseline medication adherence score, as measured by the 4‐item Morisky score, there was a 1.6% absolute increase in postdischarge medication adherence (95% CI 0.8% to 2.4%). Surprisingly, health literacy was not an independent predictor of postdischarge adherence.CONCLUSIONS:In patients hospitalized for cardiovascular disease, predictors of lower medication adherence postdischarge included younger age, Medicaid insurance, and baseline nonadherence. These factors can help predict patients who may benefit from further interventions. Journal of Hospital Medicine 2012;. © 2012 Society of Hospital Medicine