bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hepatosplenic T‐cell lymphoma is a distinct rare entity: Diagnosis by fine‐needle aspiration cytology
Authors: Kumar Rajiv , Dey Pranab , Das Ashim , Sachdeva Manupdesh Singh , Varma, Subhash 
Published: November 2010

AbstractIn this brief report, we have described the fine‐needle aspiration cytology (FNAC) of a hepatosplenic T‐cell lymphoma (HSTCL) in a 60‐year‐old female patient. The patient complained of fever and heaviness of left upper abdomen. Ultrasound‐guided FNAC of the spleen showed immature lymphoid cells, and a diagnosis of primary non‐Hodgkin lymphoma of spleen was offered. Subsequently, the patient underwent splenectomy and wedge biopsy of liver that confirmed the cytological diagnosis. The lymphoma cells were positive for CD3 immunostain. Primary hepatosplenic T‐cell lymphoma is a rare entity and may often pose diagnostic problems on FNAC. Diagn. Cytopathol. 2010. © 2010 Wiley‐Liss, Inc.
Xenopus er71 is involved in vascular development
Authors: Neuhaus Herbert , Müller Frank , Hollemann Thomas 
Published: November 2010

AbstractVasculogenesis and hematopoiesis are closely linked in developing vertebrates. Recently, the existence of a common progenitor of these two tissues, the hemangioblast, has been demonstrated in different organisms. In Xenopus early vascular and hematopoietic cells differentiate in a region called the anterior ventral blood island (aVBI). Differentiating cells from this region migrate out to form embryonic blood and part of the vascular structures of the early frog embryo. A number of members of the ETS family of transcription factors are expressed in endothelial cells and some of them play important roles at various stages of vascular development. The loss of ER71 function in mice led to a complete loss of blood and vascular structures. Similarly, knock down of the zebrafish homolog of er71, etsrp, greatly affected development of vascular structures and myeloid cells. We have identified the Xenopus ortholog of er71 and could show that er71 function in Xenopus is required for vasculogenesis, but not for the development of hematopoietic cells. Developmental Dynamics 239:3436–3445, 2010. © 2010 Wiley‐Liss, Inc.
Hydrologic, abiotic and biotic interactions: plant density, windspeed, leaf size and groundwater all affect oak water use efficiency
Authors: Law Darin J. , Finch Deborah M. 
Published: November 2010

AbstractPlant water use in drylands can be complex due to variation in hydrologic, abiotic and biotic factors, particularly near ephemeral or intermittent streams. Plant use of groundwater may be important but is usually uncertain. Disturbances like fire contribute to complex spatiotemporal heterogeneity. Improved understanding of how such hydrologic, abiotic, and biotic factors affect plant water use is needed not only to address management issues related to land use but also due to climate change, particularly for drylands projected to become warmer and drier such as much of the southwestern USA. Here we focus on the interactive roles of hydrologic, abiotic, and biotic factors in determining plant water use of Gambel oak in ephemeral and/or intermittent riparian areas of ponderosa pine forest in central New Mexico, USA along a fire disturbance gradient with varying oak densities. More specifically, the purpose of this research is to determine: (1) depth to and frequency of groundwater associated with intermittent and ephemeral streams, (2) isotopic indications of whether or not groundwater is used by plants, and (3) plant water use (conductance, transpiration, and water use efficiency) interactions with groundwater. Depth to groundwater, which could be as shallow as 1 m or less, increased with plant density but plant water use efficiency decreased. Photosynthesis:transpiration ratios maximizing water use efficiency for a given successional stage appeared to be also affected by windspeed and leaf size, highlighting interactive effects of hydrologic, abiotic and biotic affects—a finding that may be ecohydrologically relevant for other dryland riparian systems. Published 2010. This article is a US Government work and is in the public domain in the USA.
Shoulder function in patients undergoing selective neck dissection with or without radiation and chemotherapy
Authors: Watkins Jeremy P. , Williams Glenn B. , Mascioli Anthony A. , Wan Jim Y. , Samant Sandeep 
Published: November 2010

AbstractBackground.Shoulder function can be negatively affected during treatment of upper aerodigestive tract carcinoma. The purpose of this study was to investigate the impact of selective neck dissection alone or in combination with radiotherapy and/or chemotherapy.Methods.We conducted an observational study in adults who underwent selective neck dissection for the treatment of upper aerodigestive tract cancer who were >6 months from completion of their therapy. Recurrent disease and use of flap reconstruction were part of the exclusion criteria. A modified Constant's score was used to evaluate shoulder function.Results.Thirty‐four patients (42 selective neck dissections) met the inclusion criterion. Operated shoulder function was significantly (p < .001) decreased as compared to the nonoperated shoulder. There was no statistical difference in the total Constant's score, in the subjective or objective scores among selective neck dissection only, in the selective neck dissection plus radiation therapy, or in the selective neck dissection plus chemoradiation therapy.Conclusion.Selective neck dissection can have a negative effect on shoulder function despite spinal accessory nerve preservation. Adjuvant therapy does not contribute additional detriment to shoulder function. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
BUBR1 expression in oral squamous cell carcinoma and its relationship to tumor stage and survival
Authors: Rizzardi Clara , Torelli Lucio , Barresi Elena , Schneider Manuela , Canzonieri Vincenzo , Biasotto Matteo , Di Lenarda Roberto , Melato Mauro 
Published: November 2010

AbstractBackground.Defects in the mitotic spindle checkpoint have been proposed to contribute to the chromosomal instability observed in human cancers, including oral squamous cell carcinoma (OSCC). BUBR1 is a key component of the spindle checkpoint, whose role in oral carcinogenesis still needs to be clarified.Methods.We have analyzed the expression of BUBR1 in 49 cases of OSCC by immunohistochemistry and compared the findings with clinicopathologic parameters, proliferative activity, and DNA ploidy.Results.BUBR1 was overexpressed in 11 cases (22.4%). Tumors with overexpression of BUBR1 were associated with a less advanced pathologic stage (p = .05) and showed longer survival periods (p = .38) but shorter recurrence‐free survival periods (p = .13) than those without it.Conclusions.Our data imply the possibility that BUBR1 may be involved in the progression of OSCC, and suggest that BUBR1 may be a promising prognostic marker in patients with OSCC. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Activation of mitogen‐activated protein kinase extracellular signal‐related kinase in head and neck squamous cell carcinomas after irradiation as part of a rescue mechanism
Authors: Affolter Annette , Fruth Kai , Brochhausen Christoph , Schmidtmann Irene , Mann Wolf J. , Brieger Jürgen 
Published: November 2010

AbstractBackground.Irradiation plays a pivotal role in head and neck squamous cell carcinoma (HNSCC) treatment. However, especially recurrent tumors frequently show increased radioresistance. We analyzed irradiation‐stimulated mitogen‐activated protein kinase (MAPK) signaling pathways to define cellular rescue mechanisms.Methods.Irradiated HNSCC cells were screened for MAPK activation and results were confirmed and refined by functional analyses. Extracellular signal‐regulated kinase (ERK) inhibitor U0126 application enabled us to specify postradiogenic cellular responses. Vascular endothelial growth factor (VEGF) levels were analyzed additionally.Results.We observed a pronounced and time‐dependent ERK stimulation. Pathway inhibition resulted in decreased radioresistance. Likewise, we found a decrease of VEGF release after inhibitor treatment. ERK activation was confirmed in xenotransplants showing elevated postradiogenic phospho‐ERK (pERK) and VEGF levels.Conclusions.Our data give evidence for induction of ERK and successive VEGF release in HNSCC during radiotherapy, which might be partially explained by autoregulated cytoprotection maintained by pERK and potentially VEGF. In conclusion, targeting the ERK–VEGF axis might enhance the efficiency of radiotherapy. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Salivary gland carcinomas in children and adolescents: A population‐based study, with comparison to adult cases
Authors: Sultan Iyad , Rodriguez–Galindo Carlos , Al‐Sharabati Sereen , Guzzo Marco , Casanova Michela , Ferrari Andrea 
Published: November 2010

AbstractBackground.Salivary gland carcinomas are rare malignancies, particularly in young individuals in whom only scanty data are available from published studies.Methods.We searched the SEER database (1973–2006) for patients with a reported diagnosis of salivary gland carcinoma; children/adolescents (<20 years old) were compared with adults.Results.We identified 263 children/adolescents (58% girls) and 12,571 adults (43% women). The most common histology was mucoepidermoid carcinoma in both groups, but the percentages of other histologies were different. Children/adolescents had more favorable features with most tumors being localized, with no extension to adjacent tissues or lymphatic spread (76% vs 50% in adults, p < .001). Also most tumors were well differentiated or moderately differentiated (88% vs 49% in adults, p < .001). The 5‐year overall survival for children/adolescents was 95% ± 1.5%, compared with 59% ± 0.5% for adults (p < .001).Conclusion.When compared with adults, salivary gland carcinomas in children/adolescents are less advanced, and have more favorable features and better outcome. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
A novel non‐CB1/TRPV1 endocannabinoid‐mediated mechanism depresses excitatory synapses on hippocampal CA1 interneurons
Authors: Edwards Jeffrey G. , Gibson Helen E. , Jensen Tyron , Nugent Fereshteh , Walther Curtis , Blickenstaff Jacob , Kauer Julie A. 
Published: November 2010

AbstractEndocannabinoids (eCBs) mediate various forms of synaptic plasticity at excitatory and inhibitory synapses in the brain. The eCB anandamide binds to several receptors including the transient receptor potential vanilloid 1 (TRPV1) and cannabinoid receptor 1 (CB1). We recently identified that TRPV1 is required for long‐term depression at excitatory synapses on CA1 hippocampal stratum radiatum interneurons. Here we performed whole‐cell patch clamp recordings from CA1 stratum radiatum interneurons in rat brain slices to investigate the effect of the eCB anandamide on excitatory synapses as well as the involvement of Group I metabotropic glutamate receptors (mGluRs), which have been reported to produce eCBs endogenously. Application of the nonhydrolysable anandamide analog R‐methanandamide depressed excitatory transmission to CA1 stratum radiatum interneurons by ∼50%. The Group I mGluR agonist DHPG also depressed excitatory glutamatergic transmission onto interneurons to a similar degree, and this depression was blocked by the mGluR5 antagonist MPEP (10 μM) but not by the mGluR1 antagonist CPCCOEt (50 μM). Interestingly, however, neither DHPG‐mediated nor R‐methanandamide‐mediated depression was blocked by the TRPV1 antagonist capsazepine (10 μM), the CB1 antagonist AM‐251 (2 μM) or a combination of both, suggesting the presence of a novel eCB receptor or anandamide target at excitatory hippocampal synapses. DHPG also occluded R‐methanandamide depression, suggesting the possibility that the two drugs elicit synaptic depression via a shared signaling mechanism. Collectively, this study illustrates a novel CB1/TRPV1‐independent eCB pathway present in the hippocampus that mediates depression at excitatory synapses on CA1 stratum radiatum interneurons. © 2010 Wiley Periodicals, Inc.
Right‐hemispheric dominance of spatial memory in split‐brain mice
Authors: Shinohara Yoshiaki , Hosoya Aki , Yamasaki Nobuyuki , Ahmed Hassan , Hattori Satoko , Eguchi Megumi , Yamaguchi Shun , Miyakawa Tsuyoshi , Hirase Hajime , Shigemoto Ryuichi 
Published: November 2010

AbstractLeft‐right asymmetry of human brain function has been known for a century, although much of molecular and cellular basis of brain laterality remains to be elusive. Recent studies suggest that hippocampal CA3‐CA1 excitatory synapses are asymmetrically arranged, however, the functional implication of the asymmetrical circuitry has not been studied at the behavioral level. In order to address the left‐right asymmetry of hippocampal function in behaving mice, we analyzed the performance of “split‐brain” mice in the Barnes maze. The “split‐brain” mice received ventral hippocampal commissure and corpus callosum transection in addition to deprivation of visual input from one eye. In such mice, the hippocampus in the side of visual deprivation receives sensory‐driven input. Better spatial task performance was achieved by the mice which were forced to use the right hippocampus than those which were forced to use the left hippocampus. In two‐choice spatial maze, forced usage of left hippocampus resulted in a comparable performance to the right counterpart, suggesting that both hippocampal hemispheres are capable of conducting spatial learning. Therefore, the results obtained from the Barnes maze suggest that the usage of the right hippocampus improves the accuracy of spatial memory. Performance of non‐spatial yet hippocampus‐dependent tasks (e.g. fear conditioning) was not influenced by the laterality of the hippocampus. © 2010 Wiley Periodicals, Inc.
Magnitude and processes of bank erosion at a small stream in Denmark
Authors: Veihe A. , Jensen N. H. , Schiøtz I. G. , Nielsen S. L. 
Published: November 2010

AbstractRiver banks are important sources of sediment and phosphorus to fluvial systems, and the erosion processes operating on the banks are complex and change over time. This study explores the magnitude of bank erosion on a cohesive streambank within a small channelized stream and studies the various types of erosion processes taking place. Repeat field surveys of erosion pin plots were carried out during a 4‐year period and observations were supplemented by continuous monitoring of volumetric soil water content, soil temperature, ground water level and exposure of a PEEP sensor. Bank erosion rates (17·6–30·1 mm year−1) and total P content on the banks were relatively high, which makes the bank an important source of sediment and phosphorus to the stream, and it was estimated that 0·27 kg Ptot year−1 ha−1 may potentially be supplied to the stream from the banks. Yearly pin erosion rates exceeding 5 cm year−1 were mainly found at the lower parts of the bank and were associated with fluvial erosion. Negative erosion pin readings were widespread with a net advance of the bank during the monitoring period mainly attributed to subaerial processes and bank failure. It was found that dry periods characterized by low soil water content and freeze–thaw cycles during winter triggered bank failures. The great spatial variability, in combination with the temporal interaction of processes operating at different scales, requires new tools such as 3‐D topographical surveying to better capture bank erosion rates. An understanding of the processes governing bank erosion is required for riparian management using vegetational measures as root size and structure play different roles when it comes to controlling bank erosion processes. Copyright © 2010 John Wiley & Sons, Ltd.
Long‐living nanobubbles of dissolved gas in aqueous solutions of salts and erythrocyte suspensions
Authors: Bunkin Nikolai F. , Ninham Barry W. , Ignatiev Pavel S. , Kozlov Valery A. , Shkirin Alexey V. , Starosvetskij Artem V. 
Published: November 2010

AbstractResults of experiments combining laser modulation interference microscopy and Mueller matrix scatterometry show that macroscopic scatterers of light are present in liquids free of external solid impurities. Experimental data on distilled water and aqueous NaCl solutions of various concentrations as well as physiological saline solution are reported. The experimental data can be interpreted by using a model of micron‐scale clusters composed of polydisperse air nanobubbles having effective radii of 70–100 nm. Their concentration increases with the growth of ionic content. We hypothesize that under certain conditions those clusters of nanobubbles can affect the erythrocyte structure. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
EBV interferes with the sensitivity of Burkitt lymphoma Akata cells to etoposide
Authors: Lima Raquel T. , Seca Hugo , Soares Paula , Nascimento Maria São José , Vasconcelos M. Helena 
Published: November 2010

AbstractBurkitt lymphoma (BL) commonly exhibits Epstein–Barr virus (EBV) positivity associated with latent chronic infection. Models of acute EBV infection have been associated with cellular resistance to apoptosis. However, the effect of latent long‐term EBV infection on apoptosis induced by drugs is not well defined. To determine this, we have studied the response of the Akata EBV+ cell line (type I latency) to etoposide, before and after downregulating EBV gene expression. We observed that downregulating EBV nuclear antigen‐1 (EBNA‐1) expression with siRNAs reverted cellular sensitivity to etoposide. In accordance with this finding, Akata EBV+ cells showed increased sensitivity to etoposide, when compared to the Akata EBV− cells. We also observed that Akata EBV+ cells presented increased apoptosis levels and decreased Bcl‐xL mRNA and protein levels, when compared to the Akata EBV− cells. In addition, Akata EBV+ cells contained less endoplasmic reticulum (ER) than EBV− cells. Finally, downregulation of EBV with EBNA‐1 siRNAs caused an increase in the expression of Bcl‐xL indicating that EBV is responsible for the differences found between the Akata EBV+ and EBV− cell lines. J. Cell. Biochem. 112: 200–210, 2011. © 2010 Wiley‐Liss, Inc.
COUP‐TFII switches responses of venous endothelium to atherosclerotic factors through controlling the profile of various inherent genes expression
Authors: Wu Xiaojing , Zou Yunzeng , Liang Yanyan , Zhou Qi , Gong Hui , Sun Aijun , Yuan Lingyan , Wang Keqiang , Ge Junbo 
Published: November 2010

AbstractEndothelial cells of arteries (AEC) have a strikingly greater responsiveness to atherosclerosis factors than venous endothelial cells (VEC). However, the reasons for this phenomenon remain unclear. Chicken ovalbumin upstream promoter‐transcription factor II (COUP‐TFII) plays an important role in regulating embryonic arterial‐venous differentiation. We therefore investigate whether COUP‐TFII is related to this different susceptibility between AEC and VEC. It is first confirmed that COUP‐TFII is expressed in VEC but not in AEC in the adult. Using a siRNA strategy, we identified the expression of Jagged1 and Notch1 in cultured human VEC, which usually exist only in AEC, after knocking down of COUP‐TFII. To further elucidate the role of COUP‐TFII, we performed DNA microarrays in VEC transfected with the siRNA of COUP‐TFII and subsequently stimulated with angiotensin II (AngII) and compared the expression profiles of 112 genes involved in various atherosclerosis‐related pathways. The results indicated that expression of atherogenic genes was significantly upregulated after AngII stimulation in VEC transfected with COUP‐TFII siRNA. Moreover, in vitro cell functional assay showed that knockdown of COUP‐TFII in VEC increased not only basal but also AngII‐induced cell adhesions. These results demonstrate that COUP‐TFII suppresses the susceptibility of VEC to atherosclerosis through controlling the expression of various atherosclerosis‐related molecules. J. Cell. Biochem. 112: 256–264, 2011. © 2010 Wiley‐Liss, Inc.
FPTB, a novel CA‐4 derivative, induces cell apoptosis of human chondrosarcoma cells through mitochondrial dysfunction and endoplasmic reticulum stress pathways
Authors: Liu Ju‐Fang , Fong Yi‐Chin , Chang Kai‐Wei , Kuo Sheng‐Chu , Chang Chih‐Shiang , Tang Chih‐Hsin 
Published: November 2010

AbstractChondrosarcoma is a malignant primary bone tumor that responds poorly to both chemotherapy and radiation therapy. The aim of this study was to elucidate the mechanism of the novel Combretastatin A‐4 derivative, 2‐(furanyl)‐5‐(pyrrolidinyl)‐1‐(3,4,5‐trimethoxybenzyl)benzoimidazole (FPTB)‐induced human chondrosarcoma cells apoptosis. FPTB induced cell apoptosis in human chondrosarcoma cell line but not primary chondrocytes. FPTB induced up‐regulation of Bax and Bak, down‐regulation of Bcl‐2 and Bcl‐XL and dysfunction of mitochondria in chondrosarcoma. FPTB also triggered endoplasmic reticulum (ER) stress, as indicated by changes in cytosol‐calcium levels. We found that FPTB increased glucose‐regulated proteins (GRP)78 but not GRP94 expression. In addition, treatment of cells with FPTB induced calpain expression and activity. Transfection of cells with GRP78 or calpain siRNA reduced FPTB‐mediated cell apoptosis. Therefore, FPTB‐induced apoptosis in chondrosarcoma cells through the mitochondria dysfunction and involves caspase‐9 and caspase‐3‐mediated mechanism. FPTB also induced cell death mediated by increasing ER stress, GPR78 activation, and Ca2+ release, which subsequently triggers calpain, caspase‐12 and caspase‐3 activity, resulting in apoptosis. J. Cell. Biochem. 112: 453–462, 2011. © 2010 Wiley‐Liss, Inc.
The role of asporin in mineralization of human dental pulp stem cells
Authors: Lee Eun‐Hyang , Park Hye‐Jeong , Jeong Ji‐Hyun , Kim Young‐Jin , Cha Du‐Won , Kwon Dae‐Keun , Lee Sang‐Han , Cho Je‐Yoel 
Published: November 2010

AbstractHuman adult dental pulp stem cells (hDPSCs) are a unique precursor population isolated from postnatal dental pulp and have the ability to regenerate a reparative dentin‐like complex. In this study, we investigated the role of Asporin in hDPSCs, which was identified as a matrix protein in our previous dentin proteomic analysis. We isolated a clonogenic, highly proliferative population of cells from adult human dental pulp. These isolated hDPSCs were confirmed by fluorescence activated cell sorting (FACS) using stem cell‐specific markers and have shown multilineage differentiation potential. The localization of Asporin was identified by immunohistochemistry in the globular calcification region in the junction of predentin and dentin. The gene and protein expression levels of Asporin were enhanced at the early stage of and then reduced during the late stage of differentiation of hDPSCs in mineralization media. ASPN knock‐down using a lentiviral system suppressed the mineralization of hDPSCs. These results suggest that ASPN plays positive roles in the mineralization of hDPSCs and predentin to dentin. J. Cell. Physiol. 226: 1676–1682, 2011. © 2010 Wiley‐Liss, Inc.
PARTNERSHIP AND INTER‐DEPENDENCE IN AID CHAINS: SOCIAL PATHOLOGY OR SOCIAL HEALTH?
Authors: Morse Stephen , McNamara Nora 
Published: November 2010

AbstractThis paper explores partnership in the Catholic Church‐aid chain. The focus for the research was the wielding of power in relationships and the notion that agencies involved in a relationship will be motivated to represent this as a partnership even if it is far from being so (a ‘social pathology’). The aid chain selected for the research comprised a number of dioceses in Nigeria and two of the major International Catholic Church‐based donors. In the Catholic Church‐based aid chain there is the time and determination to work matters out, which suggests inter‐dependency between all involved and avoidance of ‘social pathology’. Copyright © 2010 John Wiley & Sons, Ltd.