bluuebunny/crossref_metadata_2025_split huggingface dataset:
Induced overexpression of protein kinase D1 stimulates mitogenic signaling in human pancreatic carcinoma PANC‐1 cells
Authors: Kisfalvi Krisztina , Hurd Cliff , Guha Sushovan , Rozengurt Enrique 
Published: January 2010

AbstractNeurotensin (NT) stimulates protein kinase D1 (PKD1), extracellular signal regulated kinase (ERK), c‐Jun N‐terminal Kinase (JNK), and DNA synthesis in the human pancreatic adenocarcinoma cell line PANC‐1. To determine the effect of PKD1 overexpression on these biological responses, we generated inducible stable PANC‐1 clones that express wild‐type (WT) or kinase‐dead (K618N) forms of PKD1 in response to the ecdysone analog ponasterone‐A (PonA). NT potently stimulated c‐Jun Ser63 phosphorylation in both wild type and clonal derivatives of PANC‐1 cells. PonA‐induced expression of WT, but not K618N PKD1, rapidly blocked NT‐mediated c‐Jun Ser63 phosphorylation either at the level of or upstream of MKK4, a dual‐specificity kinase that leads to JNK activation. This is the first demonstration that PKD1 suppresses NT‐induced JNK/cJun activation in PANC‐1 cells. In contrast, PKD1 overexpression markedly increased the duration of NT‐induced ERK activation in these cells. The reciprocal influence of PKD1 signaling on pro‐mitogenicERK and pro‐apopotic JNK/c‐Jun pathways prompted us to examine whether PKD1 overexpression promotes DNA synthesis and proliferation of PANC‐1 cells. Our results show that PKD1 overexpression increased DNA synthesis and cell numbers of PANC‐1 cells cultured in regular dishes or in polyhydroxyethylmethacrylate [Poly‐(HEMA)]‐coated dishes to eliminate cell adhesion (anchorage‐independent growth). Furthermore, PKD1 overexpression markedly enhanced DNA synthesis induced by NT (1–10 nM). These results indicate that PKD1 mediates mitogenic signaling in PANC‐1 and suggests that this enzyme could be a novel target for the development of therapeutic drugs that restrict the proliferation of these cells. J. Cell. Physiol. 223: 309–316, 2010. © 2010 Wiley‐Liss, Inc.
DNA stable pentaploid H1 (ES) cells obtained from an octaploid cell induced from tetraploid cells polyploidized using demecolcine
Authors: Fujikawa‐Yamamoto Kohzaburo , Luo Xianwen , Miyagoshi Minoru , Yamagishi Hiroko 
Published: January 2010

AbstractPentaploid H1 (ES) cells (5H1 cells) were accidentally obtained through one‐cell cloning of octaploid H1 (ES) cells (8H1 cells) that were established from tetraploid H1 (ES) cells (4H1 cells) polyploidized using demecolcine. The number of chromosomes of 5H1 cells was 100, unlike the 40 of diploid H1 (ES) cells (2H1 cells), 80 of 4H1, and 160 of 8H1 cells. The durations of G1, S, and G2/M phases of 5H1 cells were 3, 7, and 6 h, respectively, almost the same as those of 2H1, 4H1, and 8H1 cells. The cell volume of 5H1 cells was half of that of 8H1 cells, suggesting that 5H1 cells were created through abnormal cell divisions of 8H1 cells. The morphology of growing 5H1 cells was a spherical cluster similar to that of 2H1 cells and differing from the flagstone‐like shape of 4H1 and 8H1 cells. Pentaploid solid tumors were formed from 5H1 cells after interperitoneal injection into the mouse abdomen, and they contained endodermal, mesodermal, and ectodermal cells as well as undifferentiated cells, suggesting both that the DNA content of 5H1 cells was retained during tumor formation and that the 5H1 cells were pluripotent. The DNA content of 5H1 cells was stable in long‐term culturing as 2H1 cells, meaning that 5H1 and 2H1 cells shared similarities in DNA structure. The excellent stability of the DNA content of 5H1 cells was explained using a hypothesis for the DNA structure of polyploid cells because the pairing of homologous chromosomes in 5H1 cells is spatially forbidden. J. Cell. Physiol. 223: 369–375, 2010. © 2010 Wiley‐Liss, Inc.
Highly efficient gene transfer system using a laminin–DNA–apatite composite layer
Authors: Oyane Ayako , Tsurushima Hideo , Ito Atsuo 
Published: January 2010

AbstractBackgroundWe have recently developed a safe and efficient gene transfer system using a laminin–DNA–apatite composite layer. The objectives of the present study were to fully characterize and optimize the laminin–DNA–apatite composite layer in relation to the efficiency of gene transfer and to demonstrate the feasibility of the composite layer in the induction of cell differentiation.MethodsThe laminin–DNA–apatite composite layer was prepared under various conditions. The efficiency of gene transfer on the resulting composite layer was evaluated using luciferase and ß‐galactosidase gene expression assay systems. A laminin–DNA–apatite composite layer, prepared under the optimized condition using a plasmid including cDNA of nerve growth factor (NGF), was then applied to the neuron‐like differentiation of PC12 cells.ResultsThe laminin content of the laminin–DNA–apatite composite layer was found to be a dominant factor improving the efficiency of gene transfer rather than the DNA content. The cell adhesion property of laminin in the composite layer should be responsible for the improvement in efficiency of gene transfer because the immobilization of albumin without the cell adhesion property in a DNA–apatite composite layer had no effect on the efficiency of gene transfer. A laminin–DNA–apatite composite layer, prepared under the optimized condition using a plasmid including cDNA of NGF, successfully induced the neuron‐like differentiation of PC12 cells.ConclusionsThe present gene transfer system, with the potential to control cell differentiation and having features of safety and relatively high and controllable efficiency, would be a useful tool for tissue engineering applications and the production of transfection microarrays. Copyright © 2010 John Wiley & Sons, Ltd.
Infinite families of crossing‐critical graphs with prescribed average degree and crossing number
Authors: Bokal Drago 
Published: January 2010

AbstractŠiráň constructed infinite families of k‐crossing‐critical graphs for every k⩾3 and Kochol constructed such families of simple graphs for every k⩾2. Richter and Thomassen argued that, for any given k⩾1 and r⩾6, there are only finitely many simple k‐crossing‐critical graphs with minimum degree r. Salazar observed that the same argument implies such a conclusion for simple k‐crossing‐critical graphs of prescribed average degree r>6. He established the existence of infinite families of simple k‐crossing‐critical graphs with any prescribed rational average degree r∈[4, 6) for infinitely many k and asked about their existence for r∈(3, 4). The question was partially settled by Pinontoan and Richter, who answered
it positively for \documentclass{article}\usepackage{amssymb}\usepackage{amsbsy}\usepackage[mathscr]{euscript}\footskip=0pc\pagestyle{empty}\begin{document}$r\in(3\frac{1}{2},4)$\end{document}.The present contribution uses two new constructions of crossing‐critical simple graphs along with the one developed by Pinontoan and Richter to unify these results and to answer Salazar's question by the following statement: there exist infinite families of simple k‐crossing‐critical graphs with any prescribed average degree r∈(3, 6), for any k greater than some lower bound Nr. Moreover, a universal lower bound NI on k applies for rational numbers in any closed interval I⊂(3, 6). © 2010 Wiley Periodicals, Inc. J Graph Theory 65: 139–162, 2010
Simple and accurate approaches to implement the complex trans‐conductance suited for time‐domain simulators for small‐signal and large‐signal table‐based models
Authors: Homayouni Seyed Majid , Schreurs Dominique , Nauwelaers Bart 
Published: January 2010

AbstractThis paper focuses on the implementation of table‐based models of high‐frequency transistors for time‐domain simulators at microwave and mm‐wave frequencies. In this frequency range, the channel is not capable of responding to the excitation instantaneously therefore, a delay‐time exists between the channel response and the channel excitation. This delay is represented by a complex trans‐conductance in terms of circuit elements. The high‐frequency models of transistors are required to have the implementation of complex trans‐conductance, where the complex part accounts mathematically for the delay‐time between the channel response and the channel excitation. This paper presents simple and accurate approaches to incorporate the complex trans‐conductance in both small‐signal and large‐signal table‐based models for time‐domain simulators (MOS‐AK International Meeting. Eindhoven, Netherlands, April 2008). Implementation approach for each model, small‐signal and large‐signal, is presented in separated sections. In the first step, the delay is realized by the introduction of an ideal transmission line between the channel excitation and the channel response. As transmission lines are not generally suitable for time‐domain simulations, a lumped element equivalent network is introduced in the second step. The latter approach is fully compatible with time‐domain simulators but frequency limitation, determined by the delay‐time value itself, is introduced. Then the implementation of the complex trans‐conductance in large‐signal model is introduced. In terms of large‐signal behavior, delay‐time is important to achieve a non‐quasi static model. Yet again there is limitation in terms of the frequency range that is determined by the delay value itself. The methodology is illustrated on the small‐signal and the large‐signal equivalent circuit of a Multi‐Fin MOSFET transistor. Simulations are carried out by Cadence Spectre and Agilent ADS simulators, and comparisons are carried out between the simulation results and the measurements. Copyright © 2010 John Wiley & Sons, Ltd.
Molecular investigation of a gene encoding organic solvent‐tolerant alkaline protease from Pseudomonas aeruginosa strain K
Authors: Abd. Rahman Raja Noor Zaliha Raja , Geok Lee Poh , Wong Chee Fah , Basri Mahiran , Salleh Abu Bakar 
Published: January 2010

AbstractA gene encoding an organic solvent‐stable protease was amplified from Pseudomonas aeruginosa strain K by polymerase chain reaction using consensus primers based on multiple sequence alignment of alkaline and metalloprotease genes from Pseudomonas species. The gene, which consisted of 1440 bp nucleotides and deduced 479 amino acid residues, was successfully expressed in pGEX‐4T‐1 expression system in the presence of 1.0 mM IPTG, after an incubation of 6 h at 37 °C. Under these conditions, the recombinant strain K protease was, subsequently, released into the periplasm of E. coli BL21 (DE3) with an optimum proteolytic activity detected at 1.0112 U/ml. To date, this is the first reported expression of alkaline protease (aprA) with such remarkable property in Escherichia coli. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Optimization of media components for laccase production by litter dwelling fungal isolate Fusarium incarnatum LD‐3
Authors: Chhaya Urvish , Gupte Akshaya 
Published: January 2010

AbstractLaccase production by solid state fermentation (SSF) using an indigenously isolated litter dwelling fungus Fusarium incarnatum LD‐3 was optimized. Fourteen medium components were screened by the initial screening method of Plackett‐Burman. Each of the components was screened on the basis of ‘p’ (probability value) which was above 95% confidence level. Ortho‐dianisidine, thiamine HCl and CuSO4 · 5 H2O were identified as significant components for laccase production. The Central Composite Design response surface methodology was then applied to further optimize the laccase production. The optimal concentration of these three medium components for higher laccase production were (g/l): CuSO4 · 5 H2O, 0.01; thiamine HCl, 0.0136 and ortho‐dianisidine, 0.388 mM served as an inducer. Wheat straw, 5.0 g was used as a solid substrate. Using this statistical optimization method the laccase production was found to increase from 40 U/g to 650 U/g of wheat straw, which was sixteen times higher than non optimized medium. This is the first report on statistical optimization of laccase production from Fusarium incarnatum LD‐3. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
GC – MS analysis reveals production of 2 – Phenylethanol from Aspergillus niger endophytic in rose
Authors: Wani Masood Ahmed , Sanjana Kaul , Kumar Dhar Manoj , Lal Dhar Kanahya 
Published: January 2010

AbstractEndophytes include all organisms that during a variable period of their life, colonize the living internal tissues of their hosts without causing detectable symptoms. Several fungal endophytes have been isolated from a variety of plant species which have proved themselves as a rich source of secondary metabolites. The reported natural products from endophytes include antibiotics, immunosuppresants, anticancer compounds, antioxidant agents, etc. For the first time Rosa damacaena (rose) has been explored for its endophytes. The rose oil industry is the major identified deligence for its application in perfumery, flavouring, ointments, and pharmaceuticals including various herbal products. During the present investigation fungal endophytes were isolated from Rosa damacaena. A total of fifty four isolates were isolated out of which sixteen isolates were screened for the production of secondary metabolites. GCMS analysis reveals the production of 2‐phenylethanol by one of the isolates JUBT 3M which was identified as Aspergillus niger. This is the first report of production of 2‐phenylethanol from endophytic A. niger. 2‐phenylethanol is an important constituent of rose oil constituting about 4.06% of rose oil. Presence of 2‐phenylethanol indicates that the endophyte of rose may duplicate the biosynthesis of phenyl propanoids by rose plant. Besides this, the other commercial applications of phenylethanol include its use in antiseptics, disinfectants, anti‐microbials and preservative in pharmaceuticals. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Development of a breast reconstruction satisfaction questionnaire (BRECON): Dimensionality and clinical importance of breast symptoms, donor site issues, patient expectations, and relationships
Authors: Temple Claire L.F. , Cook E. Francis , Ross Douglas C. , Bettger‐Hahn Margo , MacDermid Joy 
Published: January 2010

AbstractObjectivesTo develop a valid, reliable and responsive, self‐administered questionnaire to assess women's satisfaction with breast reconstruction.MethodsItem generation: Three sources for item inventory were utilized: focus groups, expert panel, and literature review.Item reduction: Item impact scores were derived from patients and experts each ranking the importance and frequency of each item. Correlation between patient and expert scores was calculated. The highest impact questions were maintained.ResultsFour focus groups comprising 20 women generated 515 items, 10 experts developed 171 items, and literature review produced 227 items. These 913 potential items were reduced to 183 by combining redundancy. The 183 items underwent formal reduction by assessing importance and frequency of each item. Thirty‐two of 40 reconstructed women and 19 of 19 experts responded to the mail‐out. Seventy‐seven items of the women's top 100 also made the experts' top 100 list. Intraclass correlation between patients and experts was 0.71 [0.62 0.77], indicating “good” but not “excellent” agreement, reinforcing the importance of patient involvement in questionnaire development. Women rated abdominal donor site issues higher than experts, and experts rated breast softness and symmetry higher than women.ConclusionsA 100‐item pilot questionnaire for breast reconstruction satisfaction was developed for psychometric testing. J. Surg. Oncol. 2010; 101:209–216. © 2010 Wiley‐Liss, Inc.
Forms of the Pasch axiom in ordered geometry
Authors: Pambuccian Victor 
Published: January 2010

AbstractWe prove that, in the framework of ordered geometry, the inner form of the Pasch axiom (IP) does not imply its outer form (OP). We also show that OP can be properly split into IP and the weak Pasch axiom (WP) (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
On pseudolinearity and generic pairs
Authors: Vassiliev Evgueni 
Published: January 2010

AbstractWe continue the study of the connection between the “geometric” properties of SU ‐rank 1 structures and the properties of “generic” pairs of such structures, started in [8]. In particular, we show that the SU‐rank of the (complete) theory of generic pairs of models of an SU ‐rank 1 theory T can only take values 1 (if and only if T is trivial), 2 (if and only if T is linear) or ω, generalizing the corresponding results for a strongly minimal T in [3]. We also use pairs to derive the implication from pseudolinearity to linearity for ω ‐categorical SU ‐rank 1 structures, established in [7], from the conjecture that an ω ‐categorical supersimple theory has finite SU ‐rank, and find a condition on generic pairs, equivalent to pseudolinearity in the general case (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
A Łoś type theorem for linear metric formulas
Authors: Bagheri Seyed‐Mohammad 
Published: January 2010

AbstractWe define an ultraproduct of metric structures based on a maximal probability charge and prove a variant of Łoś theorem for linear metric formulas. We also consider iterated ultraproducts (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Tallness and level by level equivalence and inequivalence
Authors: Apter Arthur W. 
Published: January 2010

AbstractWe construct two models containing exactly one supercompact cardinal in which all non‐supercompact measurable cardinals are strictly taller than they are either strongly compact or supercompact. In the first of these models, level by level equivalence between strong compactness and supercompactness holds. In the other, level by level inequivalence between strong compactness and supercompactness holds. Each universe has only one strongly compact cardinal and contains relatively few large cardinals (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Loss of syndecan‐1 is associated with malignant conversion in skin carcinogenesis
Authors: Stepp Mary Ann , Pal‐Ghosh Sonali , Tadvalkar Gauri , Rajjoub Lamise , Jurjus Rosalyn A. , Gerdes Michael , Ryscavage Andrew , Cataisson Christophe , Shukla Anjali , Yuspa Stuart H. 
Published: January 2010

AbstractSyndecan‐1 (sdc‐1) is a cell surface proteoglycan that mediates the interaction of cells with their matrix, influencing attachment, migration, and response to growth factors. In keratinocytes, loss of sdc‐1 delays wound healing, reduces migration, and increases Transforming growth factor β (TGFβ) 1 expression. In this study we show that sdc‐1 expression is significantly reduced in basal cell, squamous cell, and metastatic human skin cancers compared to normal human skin. In experimental mouse skin tumor induction, compared to wildtype (wt) BALB/c mice, papilloma formation in sdc‐1 null mice was reduced by 50% and the percent of papillomas converting to squamous cell carcinoma (SCC) was enhanced. sdc‐1 expression on wt mouse papillomas decreased as they converted to SCC. Furthermore, papillomas forming on sdc‐1 null mice expressed suprabasal α3 and β4 integrins; suprabasal β4 integrin is a marker of a high risk for progression. While the proliferative response to phorbol‐12‐myristate‐13‐acetate (TPA) did not differ among the genotypes, sdc‐1 null mice had an enhanced inflammatory response and retained higher levels of total TGFβ1 within their skin after TPA treatment. sdc‐1 null keratinocytes, transduced in vitro by oncogenic rasHa, expressed higher levels of β4 integrin and had enhanced pSmad2 signaling and reduced senescence when compared to wt rasHa‐transduced keratinocytes. When rasHa‐transduced cells of both genotypes were grafted onto nude mice, null tumors converted to SCC with higher frequency confirming the skin painting experiments. These data indicate that sdc‐1 is important both early in the development of skin tumors and in progression of skin cancers suggesting that reduced expression of sdc‐1 could be a useful marker for progression in neoplastic skin lesions. © 2010 Wiley‐Liss, Inc.
Leucine attenuates skeletal muscle wasting via inhibition of ubiquitin ligases
Authors: Baptista Igor L. , Leal Marcelo L. , Artioli Guilherme G. , Aoki Marcelo S. , Fiamoncini Jarlei , Turri Antonio O. , Curi Rui , Miyabara Elen H. , Moriscot Anselmo S. 
Published: January 2010

AbstractThe aim of this study was to assess the effect of leucine supplementation on elements of the ubiquitin–proteasome system (UPS) in rat skeletal muscle during immobilization. This effect was evaluated by submitting the animals to a leucine supplementation protocol during hindlimb immobilization, after which different parameters were determined, including: muscle mass; cross‐sectional area (CSA); gene expression of E3 ligases/deubiquitinating enzymes; content of ubiquitinated proteins; and rate of protein synthesis. Our results show that leucine supplementation attenuates soleus muscle mass loss driven by immobilization. In addition, the marked decrease in the CSA in soleus muscle type I fibers, but not type II fibers, induced by immobilization was minimized by leucine feeding. Interestingly, leucine supplementation severely minimized the early transient increase in E3 ligase [muscle ring finger 1 (MuRF1) and muscle atrophy F‐box (MAFbx)/atrogin‐1] gene expression observed during immobilization. The reduced peak of E3 ligase gene expression was paralleled by a decreased content of ubiquitinated proteins during leucine feeding. The protein synthesis rate decreased by immobilization and was not affected by leucine supplementation. Our results strongly suggest that leucine supplementation attenuates muscle wasting induced by immobilization via minimizing gene expression of E3 ligases, which consequently could downregulate UPS‐driven protein degradation. It is notable that leucine supplementation does not restore decreased protein synthesis driven by immobilization. Muscle Nerve, 2010
L1 radiculopathy mimicking meralgia paresthetica: A case report
Authors: Yang Seung Nam , Kim Dong Hwee 
Published: January 2010

AbstractL1 radiculopathy is very rare and difficult to diagnose with needle electromyography. A patient presented with pain and hypesthesia on the anterolateral aspect of the left thigh. Nerve conduction studies and needle electromyography were normal, except for the quadratus lumborum and iliopsoas muscles, which showed abnormal spontaneous activity and polyphasic motor unit potentials with reduced recruitment patterns. Magnetic resonance imaging of the lumbar spine showed disc extrusion of the L1–2 intervertebral space with upward migration. This case demonstrates the usefulness of examination of the quadratus lumborum in the diagnosis of L1 radiculopathy. Muscle Nerve 41: 566–568, 2010