bluuebunny/crossref_metadata_2025_split huggingface dataset:
Significance of nitrosative stress and glycoxidation products in the diagnosis of COVID-19
Authors: Wolszczak-Biedrzycka Blanka , Dorf Justyna , Matowicka-Karna Joanna , Wojewódzka-Żeleźniakowicz Marzena , Żukowski Piotr , Zalewska Anna , Maciejczyk Mateusz 
Published: April 2024

AbstractNitrosative stress promotes protein glycoxidation, and both processes can occur during an infection with the SARS-CoV-2 virus. Therefore, the aim of this study was to assess selected nitrosative stress parameters and protein glycoxidation products in COVID-19 patients and convalescents relative to healthy subjects, including in reference to the severity of COVID-19 symptoms. The diagnostic utility of nitrosative stress and protein glycoxidation biomarkers was also evaluated in COVID-19 patients. The study involved 218 patients with COVID-19, 69 convalescents, and 48 healthy subjects. Nitrosative stress parameters (NO, S-nitrosothiols, nitrotyrosine) and protein glycoxidation products (tryptophan, kynurenine, N-formylkynurenine, dityrosine, AGEs) were measured in the blood plasma or serum with the use of colorimetric/fluorometric methods. The levels of NO (p = 0.0480), S-nitrosothiols (p = 0.0004), nitrotyrosine (p = 0.0175), kynurenine (p < 0.0001), N-formylkynurenine (p < 0.0001), dityrosine (p < 0.0001), and AGEs (p < 0.0001) were significantly higher, whereas tryptophan fluorescence was significantly (p < 0.0001) lower in COVID-19 patients than in the control group. Significant differences in the analyzed parameters were observed in different stages of COVID-19. In turn, the concentrations of kynurenine (p < 0.0001), N-formylkynurenine (p < 0.0001), dityrosine (p < 0.0001), and AGEs (p < 0.0001) were significantly higher, whereas tryptophan levels were significantly (p < 0.0001) lower in convalescents than in healthy controls. The ROC analysis revealed that protein glycoxidation products can be useful for diagnosing infections with the SARS-CoV-2 virus because they differentiate COVID-19 patients (KN: sensitivity—91.20%, specificity—92.00%; NFK: sensitivity—92.37%, specificity—92.00%; AGEs: sensitivity—99,02%, specificity—100%) and convalescents (KN: sensitivity—82.22%, specificity—84.00%; NFK: sensitivity—82,86%, specificity—86,00%; DT: sensitivity—100%, specificity—100%; AGE: sensitivity—100%, specificity—100%) from healthy subjects with high sensitivity and specificity. Nitrosative stress and protein glycoxidation are intensified both during and after an infection with the SARS-CoV-2 virus. The levels of redox biomarkers fluctuate in different stages of the disease. Circulating biomarkers of nitrosative stress/protein glycoxidation have potential diagnostic utility in both COVID-19 patients and convalescents.
A method for managing scientific research project resource conflicts and predicting risks using BP neural networks
Authors: Dong Xuying , Qiu Wanlin 
Published: April 2024

AbstractThis study begins by considering the resource-sharing characteristics of scientific research projects to address the issues of resource misalignment and conflict in scientific research project management. It comprehensively evaluates the tangible and intangible resources required during project execution and establishes a resource conflict risk index system. Subsequently, a resource conflict risk management model for scientific research projects is developed using Back Propagation (BP) neural networks. This model incorporates the Dropout regularization technique to enhance the generalization capacity of the BP neural network. Leveraging the BP neural network’s non-linear fitting capabilities, it captures the intricate relationship between project resource demand and supply. Additionally, the model employs self-learning to continuously adapt to new scenarios based on historical data, enabling more precise resource conflict risk assessments. Finally, the model’s performance is analyzed. The results reveal that risks in scientific research project management primarily fall into six categories: material, equipment, personnel, financial, time, and organizational factors. This study’s model algorithm exhibits the highest accuracy in predicting time-related risks, achieving 97.21%, surpassing convolutional neural network algorithms. Furthermore, the Root Mean Squared Error of the model algorithm remains stable at approximately 0.03, regardless of the number of hidden layer neurons, demonstrating excellent fitting capabilities. The developed BP neural network risk prediction framework in this study, while not directly influencing resource utilization efficiency or mitigating resource conflicts, aims to offer robust data support for research project managers when making decisions on resource allocation. The framework provides valuable insights through sensitivity analysis of organizational risks and other factors, with their relative importance reaching up to 20%. Further research should focus on defining specific strategies for various risk factors to effectively enhance resource utilization efficiency and manage resource conflicts.
Intranasal oxytocin suppresses seizure-like behaviors in a mouse model of NGLY1 deficiency
Authors: Makita Yukimasa , Asahina Makoto , Fujinawa Reiko , Yukitake Hiroshi , Suzuki Tadashi 
Published: April 2024

AbstractNGLY1 deficiency is a genetic disease caused by biallelic mutations of the Ngly1 gene. Although epileptic seizure is one of the most severe symptoms in patients with NGLY1 deficiency, preclinical studies have not been conducted due to the lack of animal models for epileptic seizures in NGLY1 deficiency. Here, we observed the behaviors of male and female Ngly1−/− mice by video monitoring and found that these mice exhibit spontaneous seizure-like behaviors. Gene expression analyses and enzyme immunoassay revealed significant decreases in oxytocin, a well-known neuropeptide, in the hypothalamus of Ngly1−/− mice. Seizure-like behaviors in Ngly1−/− mice were transiently suppressed by a single intranasal administration of oxytocin. These findings suggest the therapeutic potential of oxytocin for epileptic seizure in patients with NGLY1 deficiency and contribute to the clarification of the disease mechanism.
Revealing the mechanisms of semantic satiation with deep learning models
Authors: Zhang Xinyu , Lian Jing , Yu Zhaofei , Tang Huajin , Liang Dong , Liu Jizhao , Liu Jian K. 
Published: April 2024

AbstractThe phenomenon of semantic satiation, which refers to the loss of meaning of a word or phrase after being repeated many times, is a well-known psychological phenomenon. However, the microscopic neural computational principles responsible for these mechanisms remain unknown. In this study, we use a deep learning model of continuous coupled neural networks to investigate the mechanism underlying semantic satiation and precisely describe this process with neuronal components. Our results suggest that, from a mesoscopic perspective, semantic satiation may be a bottom-up process. Unlike existing macroscopic psychological studies that suggest that semantic satiation is a top-down process, our simulations use a similar experimental paradigm as classical psychology experiments and observe similar results. Satiation of semantic objectives, similar to the learning process of our network model used for object recognition, relies on continuous learning and switching between objects. The underlying neural coupling strengthens or weakens satiation. Taken together, both neural and network mechanisms play a role in controlling semantic satiation.
Single-cell T-cell receptor repertoire profiling in dogs
Authors: Hoang My H. , Skidmore Zachary L. , Rindt Hans , Chu Shirley , Fisk Bryan , Foltz Jennifer A. , Fronick Catrina , Fulton Robert , Zhou Mingyi , Bivens Nathan J. , Reinero Carol N. , Fehniger Todd A. , Griffith Malachi , Bryan Jeffrey N. , Griffith Obi L. 
Published: April 2024

AbstractSpontaneous cancers in companion dogs are robust models of human disease. Tracking tumor-specific immune responses in these models requires reagents to perform species-specific single cell T cell receptor sequencing (scTCRseq). scTCRseq and integration with scRNA data have not been demonstrated on companion dogs with cancer. Here, five healthy dogs, two dogs with T cell lymphoma and four dogs with melanoma are selected to demonstrate applicability of scTCRseq in a cancer immunotherapy setting. Single-cell suspensions of PBMCs or lymph node aspirates are profiled using scRNA and dog-specific scTCRseq primers. In total, 77,809 V(D)J-expressing cells are detected, with an average of 3498 (348 - 5,971) unique clonotypes identified per sample. In total, 29/34, 40/40, 22/22 and 9/9 known functional TRAV, TRAJ, TRBV and TRBJ gene segments are observed respectively. Pseudogene or otherwise defective gene segments are also detected supporting re-annotation of several as functional. Healthy dogs exhibit highly diverse repertoires, T cell lymphomas exhibit clonal repertoires, and vaccine-treated melanoma dogs are dominated by a small number of highly abundant clonotypes. scRNA libraries define large clusters of V(D)J-expressing CD8+ and CD4 + T cells. Dominant clonotypes observed in melanoma PBMCs are predominantly CD8 + T cells, with activated phenotypes, suggesting possible anti-tumor T cell populations.
Disulfiram treatment suppresses antibody-producing reactions by inhibiting macrophage activation and B cell pyrimidine metabolism
Authors: Chen Weili , Toda Etsuko , Takeuchi Kazuhiro , Sawa Yurika , Wakamatsu Kyoko , Kuwahara Naomi , Ishikawa Arimi , Igarashi Yuri , Terasaki Mika , Kunugi Shinobu , Terasaki Yasuhiro , Yamada Kazuhiko , Terashima Yuya , Shimizu Akira 
Published: April 2024

AbstractAntibody responses, involving B cells, CD4 + T cells, and macrophages, are implicated in autoimmune diseases and organ transplant rejection. We have previously shown that inhibiting FROUNT with disulfiram (DSF) suppresses macrophage activation and migration, effectively treating inflammatory diseases. In this study, we investigated the effectiveness of DSF in antibody-producing reactions. Using a heart transplantation mouse model with antibody-mediated rejection, we administered anti-CD8 antibody to exclude cellular rejection. DSF directly inhibited B cell responses in vitro and significantly reduced plasma donor-specific antibodies and graft antibody deposition in vivo, resulting in prolonged survival of the heart graft. DSF also mediated various effects, including decreased macrophage infiltration and increased Foxp3+ regulatory T-cells in the grafts. Additionally, DSF inhibited pyrimidine metabolism-related gene expression induced by B-cell stimulation. These findings demonstrate that DSF modulates antibody production in the immune response complexity by regulating B-cell and macrophage responses.
Urban flood risk management needs nature-based solutions: a coupled social-ecological system perspective
Authors: Zhou Kejing , Kong Fanhua , Yin Haiwei , Destouni Georgia , Meadows Michael E. , Andersson Erik , Chen Liding , Chen Bin , Li Zhenya , Su Jie 
Published: April 2024

AbstractA growing number of Nature-based Solutions (NbS) has been advocated for urban flood risk management (FRM). However, whether NbS for FRM (NbS-FRM) achieves both social and ecological co-benefits remains largely unknown. We here propose and use a conceptual framework with a coupled social-ecological perspective to explore and identify such “win-win” potential in NbS-FRM. Through a scoping-review we find that ecological FRM measures are unevenly distributed around the world, and those solely targeting flood mitigation may have unintended negative consequences for society and ecosystems. In elaborating this framework with evidence from the reviewed studies, we find that NbS-FRM has the potential to provide both social and ecological co-benefits, with remaining gaps including a lack of resilience thinking, inadequate consideration of environmental changes, and limited collaborative efforts to manage trade-offs. The proposed framework shows how to move forward to leverage NbS for equitable and sustainable FRM with improved human well-being and ecosystem health.
A checkpoint function for Nup98 in nuclear pore formation suggested by novel inhibitory nanobodies
Authors: Solà Colom Mireia , Fu Zhenglin , Gunkel Philip , Güttler Thomas , Trakhanov Sergei , Srinivasan Vasundara , Gregor Kathrin , Pleiner Tino , Görlich Dirk 
Published: April 2024

AbstractNuclear pore complex (NPC) biogenesis is a still enigmatic example of protein self-assembly. We now introduce several cross-reacting anti-Nup nanobodies for imaging intact nuclear pore complexes from frog to human. We also report a simplified assay that directly tracks postmitotic NPC assembly with added fluorophore-labeled anti-Nup nanobodies. During interphase, NPCs are inserted into a pre-existing nuclear envelope. Monitoring this process is challenging because newly assembled NPCs are indistinguishable from pre-existing ones. We overcame this problem by inserting Xenopus-derived NPCs into human nuclear envelopes and using frog-specific anti-Nup nanobodies for detection. We further asked whether anti-Nup nanobodies could serve as NPC assembly inhibitors. Using a selection strategy against conserved epitopes, we obtained anti-Nup93, Nup98, and Nup155 nanobodies that block Nup–Nup interfaces and arrest NPC assembly. We solved structures of nanobody-target complexes and identified roles for the Nup93 α-solenoid domain in recruiting Nup358 and the Nup214·88·62 complex, as well as for Nup155 and the Nup98 autoproteolytic domain in NPC scaffold assembly. The latter suggests a checkpoint linking pore formation to the assembly of the Nup98-dominated permeability barrier.
Ago2/CAV1 interaction potentiates metastasis via controlling Ago2 localization and miRNA action
Authors: Lin Meng-Chieh , Kuo Wen-Hung , Chen Shih-Yin , Hsu Jing-Ya , Lu Li-Yu , Wang Chen-Chi , Chen Yi-Ju , Tsai Jia-Shiuan , Li Hua-Jung 
Published: April 2024

AbstractAgo2 differentially regulates oncogenic and tumor-suppressive miRNAs in cancer cells. This discrepancy suggests a secondary event regulating Ago2/miRNA action in a context-dependent manner. We show here that a positive charge of Ago2 K212, that is preserved by SIR2-mediated Ago2 deacetylation in cancer cells, is responsible for the direct interaction between Ago2 and Caveolin-1 (CAV1). Through this interaction, CAV1 sequesters Ago2 on the plasma membranes and regulates miRNA-mediated translational repression in a compartment-dependent manner. Ago2/CAV1 interaction plays a role in miRNA-mediated mRNA suppression and in miRNA release via extracellular vesicles (EVs) from tumors into the circulation, which can be used as a biomarker of tumor progression. Increased Ago2/CAV1 interaction with tumor progression promotes aggressive cancer behaviors, including metastasis. Ago2/CAV1 interaction acts as a secondary event in miRNA-mediated suppression and increases the complexity of miRNA actions in cancer.
Teleaudiology Services in Australia: A National Survey of Hearing Health Care Consumers Amid the COVID-19 Pandemic
Authors: Kelsall-Foreman India , Bacusmo Eloise Anne Z. , Barr Caitlin , Vitkovic Jessica , Campbell Elissa , Coles Tony , Paton Mark , Penno Kathryn , Bennett Rebecca J. 
Published: April 2024

Purpose:
The aim of this study was to investigate the perspectives of Australia-based hearing health care consumers regarding the (a) provision and utilization of teleaudiology services, (b) experiences with teleaudiology, and (c) barriers and enablers to future teleaudiology use.

Method:

A national prospective self-report online survey was completed between April and October 2020, amid COVID-19. Data were analyzed using descriptive statistics (closed-answer items) and content analysis (open-text responses). A total of 381 participants (
M
age
= 72.07 years ± 10.08, 142 females) were recruited from different states and territories of Australia.

Results:
Despite positive outcomes reported by those who undertook teleaudiology appointments during COVID-19, results indicate low-consumer teleaudiology uptake. It can be inferred that consumers were not aware of teleaudiology as an appointment option, clinicians/clinic staff had not informed and/or supported teleaudiology as an option, and biases existed that prevented teleaudiology being more widely adopted. It is unclear whether consumers who were eligible for government subsidies understood that teleaudiology appointments were reimbursed through government funding. Barriers to future teleaudiology uptake were largely related to concerns regarding confidentiality and privacy.

Conclusion:
Low consumer uptake of teleaudiology appointments appears to be driven by consumer preference for in-person services, which appears to be driven by lack of knowledge regarding the availability and effectiveness of teleaudiology.

Supplemental Material:

https://doi.org/10.23641/asha.25619808
Pedagogical Approaches to Cultural and Linguistic Diversity in Communication Sciences and Disorders Programs: A Nationwide Survey
Authors: Higby Eve , Castillo Maria Maria , Aranda Alexia , Nicholas Katrina , Datta Hia 
Published: April 2024

Purpose:
This study investigates how instructors in communication sciences and disorders (CSD) programs teach about cultural and linguistic diversity (CLD) in general-content courses as well as those dedicated to CLD content with the goal of identifying ways of improving training of preservice clinicians to provide culturally responsive service delivery.

Method:
A survey was sent to 4,192 instructors in CSD programs at 295 institutions in the United States and Puerto Rico, of which 565 responded. The survey contained choice response questions and open-ended questions.

Results:
Curricular infusion as the sole strategy of CLD training has decreased since a similar survey was published by Stockman et al. (2008), while the number of programs that offer courses dedicated to CLD or use both strategies has increased. Dedicated CLD courses offer broader training in CLD issues and are considered more effective at preparing students to work with CLD populations. A number of challenges were identified, such as a lack of available resources or time to cover CLD issues.

Conclusions:
A combination of curricular infusion and dedicated CLD coursework is recommended to ensure sufficient training in CLD issues. The development of additional resources is needed to better support instructors in both general-content and CLD-dedicated courses. Finally, CLD training should engage students in challenging unjust systems and harmful ideologies and not just celebrate multiculturalism in order to provide culturally responsive service to all clients.

Supplemental Material:

https://doi.org/10.23641/asha.25587651
Physical properties of the southwest outflow streamer in the starburst galaxy NGC 253 with ALCHEMI
Authors: Bao Min , Harada Nanase , Kohno Kotaro , Yoshimura Yuki , Egusa Fumi , Nishimura Yuri , Tanaka Kunihiko , Nakanishi Kouichiro , Martín Sergio , Mangum Jeffrey G. , Sakamoto Kazushi , Muller Sébastien , Bouvier Mathilde , Colzi Laura , Emig Kimberly L. , Meier David S. , Henkel Christian , Humire Pedro , Huang Ko-Yun , Rivilla Víctor M. , van der Werf Paul , Viti Serena 
Published: April 2024

Aims. The physical properties of galactic molecular outflows are important as they could constrain outflow formation mechanisms. In this work, we study the properties of the southwest (SW) outflow streamer including gas kinematics, optical depth, dense gas fraction, and shock strength through molecular emission in the central molecular zone of the starburst galaxy NGC 253.
Methods. We imaged the molecular emission in NGC 253 at a spatial resolution of 1.6″(∼27 pc at D ∼ 3.5 Mpc) based on data from the ALMA Comprehensive High-resolution Extragalactic Molecular Inventory (ALCHEMI) large program. We traced the velocity and velocity dispersion of molecular gas with the CO(1–0) line and studied the molecular spectra in the region of the SW streamer, the brightest CO streamer in NGC 253. We constrained the optical depth of the CO emission with the CO/13CO(1–0) ratio, the dense gas fraction with the HCN/CO(1–0), H13CN/13CO(1–0) and N2H+/13CO(1–0) ratios, as well as the shock strength with the SiO(2–1)/13CO(1–0) and CH3OH(2k–1k)/13CO(1–0) ratios.
Results. The CO/13CO(1–0) integrated intensity ratio is ∼21 in the SW streamer region, which approximates the C/13C isotopic abundance ratio. The higher integrated intensity ratio compared to the disk can be attributed to the optically thinner environment of CO(1–0) emission inside the SW streamer. The HCN/CO(1–0) and SiO(2–1)/13CO(1–0) integrated intensity ratios both approach ∼0.2 in three giant molecular clouds (GMCs) at the base of the outflow streamers, which implies a higher dense gas fraction and strength of fast shocks in those GMCs than in the disk, while the HCN/CO(1–0) integrated intensity ratio is moderate in the SW streamer region. The contours of those two integrated intensity ratios are extended in the directions of outflow streamers, which connect the enhanced dense gas fraction and shock strength with molecular outflow. Moreover, the molecular gas with an enhanced dense gas fraction and shock strength located at the base of the SW streamer shares the same velocity as the outflow.
Conclusions. The enhanced dense gas fraction and shock strength at the base of the outflow streamers suggest that star formation inside the GMCs can trigger shocks and further drive the molecular outflow. The increased CO/13CO(1–0) integrated intensity ratio coupled with the moderate HCN/CO(1–0) integrated intensity ratio in the SW streamer region are consistent with the picture that the gas velocity gradient inside the streamer may decrease the optical depth of CO(1–0) emission, as well as the dense gas fraction in the extended streamer region.
Phase angle dependence of the dust cross section in a cometary coma
Authors: Keiser F. , Markkanen J. , Agarwal J. 
Published: April 2024

Context. Rosetta/OSIRIS took optical measurements of the intensity of scattered light from the coma of 67P/Churyumov-Gerasimenko over a wide range of phase angles. These data have been used to measure the phase angle dependent radiance profile of the dust coma.
Aims. We want to provide information about the column area densities of the dust coma as seen from Rosetta. This information in combination with the measured OSIRIS phase function can then be used to determine the scattering phase function of the dust particles.
Methods. We use a simple numerical model to calculate the dust density in the coma. For this we neglect all forces but solar gravitation and radiation pressure. As this cannot describe particles close to the surface of the comet, we assume starting conditions at a sufficient distance. We evaluate the column area density as observed from Rosetta/OSIRIS and compare the results for different spacecraft positions, dust sizes and surface activity distributions.
Results. We find the phase angle dependence of the column area density to be largely independent of particle size and spacecraft positions. The determining factor is the activity distribution across the surface, especially the activity on the night side. For models with no night side activity, we find the column area density at high phase angles to be roughly two orders of magnitude larger than at low phase angles.
Conclusions. The radiance profile measured from inside a cometary coma results from the combined effects of a phase angle dependent column area density and the scattering phase function. The radiance profile is therefore strongly dependent on the surface activity distribution, and - unless the dust emission is isotropic - any attempt to infer particle properties (as expressed through the scattering phase function) from such data must take into account and de-bias for this spatial variation of the dust column area density.