bluuebunny/crossref_metadata_2025_split huggingface dataset:
Crush Stenting With Drug‐Eluting Stents: Relevance of Coronary Bifurcation Lesion Location on Angiographic and Clinical Outcomes
Authors: Chen Shao‐Liang , Zhang Jun‐jie , Ye Fei , Liu Zhi‐zhong , Zhu Zhong‐sheng , Lin Song , Tian Nai‐liang , Fang Wei‐yi , Chen Yun‐dai , Sun Xue‐wen , Wei Meng , Shan Shou‐jie , Kan Jing , Qian Jun , Yang Song , Yuan Zeng‐bai , Kwan Tak W. , Hu Da‐Yi 
Published: September 2010

AbstractBackgroundData on the relevance of the location of coronary bifurcation lesions treated by crush stenting with outcomes were limited.HypothesisWe hypothesized that the location of the bifurcation lesion correlated with clinical outcome.MethodA total of 212 patients with 230 true bifurcation lesions treated by crush stenting with drug‐eluting stents (DES) were assessed prospectively. Surveillance quantitative angiographies were indexed at 8 months after procedure. Primary endpoint was major adverse cardiac events (MACE), defined as cardiac death, myocardial infarction, and target lesion revascularization (TLR).ResultsPatients in the distal right coronary artery (RCAd) group were characterized by higher proportions of prior myocardial infarction and very tortuous lesions. However, lesions in the RCAd group, compared to those of other groups, had the lowest late lumen loss, with resultant lowest incidence of MACE at a mean follow‐up of 268±35 days. Independent predictors of MACE included unsatisfied kissing (KUS; hazard ratio [HR]: 12.14, 95% confidence interval [CI]: 4.01–12.10, P = .001) and non‐RCA lesion (HR: 20.69, 95% CI: 5.05–22.38, P = .001), while those of TLR were KUS (HR: 10.21, 95% CI: 0.01–0.34, P = .002), bifurcation angle (HR: 4.728, 95% CI: 2.541–4.109, P = .001), and non‐RCA lesion (HR: 16.05, 95%CI: 1.01–4.83, P = .001).ConclusionsClassical crush stenting with drug‐eluting stents is associated with significantly better outcomes in RCAd. Quality of kissing inflation is mandatory to improve outcome. Copyright © 2009 Wiley Periodicals, Inc.
State‐of‐the‐art process for the safe synthesis of anticancer drugs
Authors: Ammoscato Vince , Wickenheiser Rebecca , Henderson John , Munk Stephen 
Published: September 2010

AbstractMost cancer drugs are quite potent and represent a potential hazard to chemists conducting their synthesis. Chemical development teams must therefore integrate proper handing techniques for such compounds into their development plans. We describe the synthesis of the drug busulfan using barrier isolation technology. These systems are a general solution for the safe handling of many potent drugs, including those used to treat cancer. Drug Dev Res 71: 439–444, 2010. © 2010 Wiley‐Liss, Inc.
Synthesis of a Chiral Palladacycle and Its Application in Asymmetric Hydrophosphanation Reactions (Eur. J. Inorg. Chem. 28/2010)
Authors: Ding Yi , Zhang Yi , Li Yongxin , Pullarkat Sumod A. , Andrews Paul , Leung Pak‐Hing 
Published: September 2010

AbstractThe cover picture shows a novel chiral palladacycle, which was readily prepared from p‐xylene and then resolved through the separation of its (S)‐prolinate diastereomeric derivatives. The catalytic ability of the newly synthesized palladacycle was demonstrated in the preparation of a new diester‐substituted diphosphane ligand by an asymmetric hydrophosphanation reaction, which proceeded with excellent selectivity. Details are discussed in the article by P.‐H. Leung et al. on p. 4427 ff. The key molecule is depicted as superimposed over the image of the Chinese Heritage Centre building at the Nanyang Technological University, Singapore, and is meant to represent the contributions of Asian authors/readers to the European Journal of Inorganic Chemistry.
Lab‐on‐a‐chip device for continuous particle and cell separation based on electrical properties via alternating current dielectrophoresis
Authors: Çetin Barbaros , Li Dongqing 
Published: September 2010

AbstractA novel alternating current‐dielectrophoresis microfluidic chip was developed to separate particles and cells continuously by their electric properties. The flow is induced by pressure gradient. A pair of simple, 3‐D electrodes was used to achieve a localized nonuniform electric field. Dielectrophoretic force is generated in the transverse direction to the flow by inserting the electrodes along the channel side walls. The localized electric field is important to reduce the Joule heating and any adverse effects of electrical field on biological cells. Latex particles of different sizes and white blood cells (8–12 μm) were manipulated successfully and the separation of 10 μm latex particles and white blood cells based on their different electrical properties was demonstrated.
“Click” chemistry‐based surface modification of poly(dimethylsiloxane) for protein separation in a microfluidic chip
Authors: Zhang Zhaowei , Feng Xiaojun , Xu Fei , Liu Xin , Liu Bi‐Feng 
Published: September 2010

Abstract“Click” chemistry‐based surface modification strategy was developed for PDMS microchips to enhance separation performance for both amino acids and proteins. Alkyne‐PEG was synthesized by a conventional procedure and then “click” grafted to azido‐PDMS. FTIR absorption by attenuated total reflection and contact angle measurements proved efficient grafting of alkyne‐PEG onto PDMS surface. Manifest EOF regulation and stability of PEG‐functionalized PDMS microchips were illustrated via EOF measurements and protein adsorption investigations. The stability of nonspecific protein adsorption resistance property was investigated up to 30 days. Separation of fluorescence‐labeled amino acids and proteins was further demonstrated with high repeatability and reproducibility. Comparison of protein separation using PDMS microchips before and after surface modification suggested greatly improved electrophoretic performance of the PEG‐functionalized PDMS microchips. We expect the “click” chemistry‐based surface modification method to have wide applications in microseparation of proteins with long‐term surface stability.
Simultaneous effects of heat and mass transfer on time‐dependent flow over a stretching surface
Authors: Hayat T. , Awais M. 
Published: September 2010

AbstractThis article deals with the combined effects of heat and mass transfer on the two‐dimensional time‐dependent flow of a second grade fluid bounded by a stretching surface. The present analysis takes into consideration the Soret and Dufour's effects in the presence of a chemical reaction. The governing equations for velocity, concentration, and temperature fields are derived. Using the homotopy analysis method, the solution of outcoming equations are computed. Graphical results for velocity, temperature, and concentration fields are reported and analyzed. Copyright © 2010 John Wiley & Sons, Ltd.
Detailed descriptions of routes traveled, but not map‐like knowledge, correlates with tests of hippocampal function in older adults
Authors: Hirshhorn Marnie , Newman Leorra , Moscovitch Morris 
Published: September 2010

AbstractWe examined hippocampal contribution to remote spatial memory in older adults by correlating their performance on tests sensitive to hippocampal damage with their description of routes they traversed many times or only once, and with their map‐like knowledge of downtown Toronto. We found that performance on table‐top tests of spatial location (Smith and Milner (1981) Neuropsychologia 19:781–793) and on paired‐associate learning, and the number of Internal Details on the Autobiographical Interview (Levine et al., (2002) Psychol Aging 17:677–689), all correlated significantly with the number and type of perceptual details used in describing routes one has traversed, but not with map‐like knowledge of Toronto. No significant correlations were found with performance on tests of frontal function (WCST, phonemic fluency, and backward digit span). We conclude that the hippocampus is implicated in vivid re‐experiencing of a familiar route, but not with map‐like knowledge of a large‐scale environment. These findings are interpreted as consistent with Multiple Trace Theory's prediction that it is the degree of detail of a retrieved memory that is crucially dependent on the hippocampus. © 2010 Wiley Periodicals, Inc.
Effects of Prandtl number on three‐dimensional mixed convection in a horizontal square duct with heated and cooled side walls
Authors: Toriyama Koji , Ichimiya Koichi 
Published: September 2010

AbstractWe examined the effects of Prandtl number on three‐dimensional mixed convection in a horizontal square duct with heated and cooled side walls numerically. Non‐dimensional governing equations were solved for Re = 100, Pr = 0.1–10, and Ri = 36.44 by the SIMPLE method. The numerical results show that the swirl flow was generated along the flow direction, and its pitch lengthened with the increase of Pr. We also examined the strength of swirl flow using the swirl number, S, and we discuss heat transfer behavior as it corresponded to the flow. Heat transfer was promoted by the swirl flow with all Pr, and the optimum value existed within these Pr. © 2010 Wiley Periodicals, Inc. Heat Trans Asian Res; Published online in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/htj.20319
Zinc deficiency activates S100A8 inflammation in the absence of COX‐2 and promotes murine oral‐esophageal tumor progression
Authors: Wan Shao‐Gui , Taccioli Cristian , Jiang Yubao , Chen Hongping , Smalley Karl J. , Huang Kun , Liu Xiu‐Ping , Farber John L. , Croce Carlo M. , Fong Louise Y. Y. 
Published: September 2010

AbstractZinc (Zn)‐deficiency (ZD) is implicated in the pathogenesis of human oral‐esophageal cancers. Previously, we showed that in ZD mice genetic deletion of cyclooxygenase‐2 (Cox‐2) enhances N‐nitrosomethylbenzylamine‐induced forestomach carcinogenesis. By contrast, Cox‐2 deletion offers protection in Zn‐sufficient (ZS) mice. We hypothesize that ZD activates pathways insensitive to COX‐2 inhibition, thereby promoting carcinogenesis. This hypothesis is tested in a Cox‐2−/− mouse tongue cancer model that mimics pharmacologic blockade of COX‐2 by firstly examining transcriptome profiles of forestomach mucosa from Cox‐2−/− and wild‐type mice on a ZD vs. ZS diet, and secondly investigating the roles of identified markers in mouse forestomach/tongue preneoplasia and carcinomas. In Cox‐2−/− mice exposed to the tongue carcinogen 4‐nitroquinoline 1‐oxide, dietary ZD elicited tongue/esophagus/forestomach carcinomas that were prevented by ZS. The precancerous ZD:Cox‐2−/−vs. ZS:Cox‐2−/− forestomach had an inflammatory signature with upregulation of the proinflammation genes S100a8 and S100a9. Bioinformatics analysis revealed overrepresentation of inflammation processes comprising S100a8/a9 and an nuclear factor (NF)‐κB network with connectivity to S100A8. Immunohistochemistry revealed co‐overexpression of S100A8, its heterodimeric partner S100A9, the receptor for advanced glycation end‐products (RAGE), NF‐κB p65, and cyclin D1, in ZD:Cox‐2−/− forestomach/tongue preneoplasia and carcinomas, evidence for the activation of a RAGE‐S100A8/A9 inflammatory pathway. Accumulation of p53 in these carcinomas indicated activation of additional inflammatory pathways. Zn‐replenishment in ZD:Cox‐2−/−mice reversed the inflammation and inhibited carcinogenesis. Thus, ZD activates alternative inflammation‐associated cancer pathways that fuel tumor progression and bypass the antitumor effect of Cox‐2 ablation. These findings have important clinical implications, as combination cancer therapy that includes Zn may improve efficacy.
Characterization of metastatic breast cancer patients with nondetectable circulating tumor cells
Authors: Mego Michal , De Giorgi Ugo , Dawood Shahenaah , Wang Xuemei , Valero Vicente , Andreopoulou Eleni , Handy Beverly , Ueno Naoto T. , Reuben James M. , Cristofanilli Massimo 
Published: September 2010

AbstractCirculating tumor cells (CTC) are an independent prognostic factor in metastatic breast cancer patients (MBC). However, CTC are undetectable in one third of patients. The aim of this study was to assess the prognostic factors in MBC patients without detectable CTC. This retrospective study included 292 MBC patients evaluated between January 2004 and December 2007. CTC were enumerated before patients started a new line of treatment using the CellSearch™. Overall survival (OS) was calculated from the date of CTC measurement and estimated by the Kaplan‐Meier product limit method. CTC were not detected in 35.96% patients, whereas 40.75% patients had CTC ≥ 5. Undetectable CTC status was positively correlated with presence of brain metastasis (OR: 6.17, 95%CI = 2.14–17.79; p = 0.001), and inversely correlated with bone metastasis (OR: 0.47; 95%CI = 0.27–0.80; p = 0.01). In multivariate analysis, hormone receptors, number of metastatic sites and lines of therapy were independent prognostic factors for OS in patients without detectable CTC. Patients without detectable CTC before starting of a new line of therapy comprise a heterogeneous group with substantially different prognosis. We showed that some important metastatic disease characteristics are predictive of undetectable CTC status in MBC.
Transient arrest in a quiescent state allows ovarian cancer cells to survive suboptimal growth conditions and is mediated by both Mirk/dyrk1b and p130/Rb2
Authors: Hu Jing , Nakhla Hassan , Friedman Eileen 
Published: September 2010

AbstractSome ovarian cancer cells in vivo are in a reversible quiescent state where they can contribute to cancer spread under favorable growth conditions. The serine/threonine kinase Mirk/dyrk1B was expressed in each of seven ovarian cancer cell lines and in 21 of 28 resected human ovarian cancers, and upregulated in 60% of the cancers. Some ovarian cancer cells were found in a G0 quiescent state, with the highest fraction in a line with an amplified Mirk gene. Suboptimal culture conditions increased the G0 fraction in SKOV3 and TOV21G, but not OVCAR4 cultures. Less than half as many OVCAR4 cells survived under suboptimal culture conditions as shown by total cell numbers, dye exclusion viability studies, and assay of cleaved apoptotic marker proteins. G0 arrest in TOV21G and SKOV3 cells led to increased levels of Mirk, the CDK inhibitor p27, p130/Rb2, and p130/Rb2 complexed with E2F4. The G0 arrest was transient, and cells exited G0 when fresh nutrients were supplied. Depletion of p130/Rb2 reduced the G0 fraction, increased cell sensitivity to serum‐free culture and to cisplatin, and reduced Mirk levels. Mirk contributed to G0 arrest by destabilization of cyclin D1. In TOV21G cells, but not in normal diploid fibroblasts, Mirk depletion led to increased apoptosis and loss of viability. Because Mirk is expressed at low levels in most normal adult tissues, the elevated Mirk protein levels in ovarian cancers may present a novel therapeutic target, in particular for quiescent tumor cells which are difficult to eradicate by conventional therapies targeting dividing cells.
MYBL2, a link between proliferation and differentiation in maturing colon epithelial cells
Authors: Papetti Michael , Augenlicht Leonard H. 
Published: September 2010

AbstractMultiple signals, controlling both proliferation and differentiation, must be integrated in the reprogramming of intestinal epithelial cells during maturation along the crypt‐luminal axis. The v‐myb family member Mybl2, a molecule implicated in the development and maintenance of the stem cell phenotype, has been suggested to play an important role in proliferation and differentiation of several cell types and is a gene we have found is commonly regulated in several systems of colon cell maturation both in vitro and in vivo. Here we show that siRNA silencing of Mybl2 in proliferating Caco‐2 cells increases expression of the cell‐cycle regulators cdk2, cyclin D2, and c‐myc and decreases expression of cdc25B and cyclin B2 with a consequent 10% increase of cells in G2/M and a complementary 10% decrease in G1. Mybl2 occupies sequences upstream of transcriptional start sites of cyclin D2, c‐myc, cyclin B2, and cdc25B and regulates reporter activity driven by upstream regions of cdk2, cyclin D2, and c‐myc. These data suggest that Mybl2 plays a subtle but key role in linking specific aspects of cell‐cycle progression with generation of signals for differentiation and may therefore be fundamental in commitment of intestinal epithelial cells to differentiation pathways during their maturation. J. Cell. Physiol. 226: 785–791, 2011. © 2010 Wiley‐Liss, Inc.
T cell suppression by osteoclasts in vitro
Authors: Grassi Francesco , Manferdini Cristina , Cattini Luca , Piacentini Anna , Gabusi Elena , Facchini Andrea , Lisignoli Gina 
Published: September 2010

AbstractT cells are critical regulators of osteoclast differentiation and function in bone, but whether osteoclasts can, in turn, regulate T cell homing, and response to stimuli is unclear. To investigate whether osteoclasts are immune competent cells, the expression of HLA Class II and costimulatory receptors was evaluated by RT‐PCR and immunohistochemistry by comparing osteoclast precursors and mature osteoclasts. T‐cell‐attracting chemokines were measured in the supernatants of confluent cultures of osteoclasts and compared with mesenchymal stromal cells and osteoblasts. T cell proliferation, cytokine production, and apoptosis were assayed in co‐cultures with osteoclasts in the presence or absence of mitogenic stimuli. To define the mechanism of action of osteoclasts, cytokine‐blocking experiments were performed. Our findings revealed that mature osteoclasts constitutively expressed Class II HLA in the membrane and upregulate the expression of CD40 and CD80 during differentiation. Osteoclasts secreted high levels of most T cell chemoattractants and effectively retained T cells in adhesion assays. Moreover, the osteoclasts potently blunted T cell response to PHA and CD3/CD28 stimulation, thus inhibiting proliferation, suppressing T cell TNFα and IFNγ production and decreasing T cell apoptosis by a mostly cell‐contact independent mechanism. In conclusion, osteoclasts are immune‐competent cells which can retain T cells and suppress in vitro T cell response to proliferative stimuli. J. Cell. Physiol. 226: 982–990, 2011. © 2010 Wiley‐Liss, Inc.
Comparative genomics of NAD(P) biosynthesis and novel antibiotic drug targets
Authors: Bi Jicai , Wang Honghai , Xie Jianping 
Published: September 2010

AbstractNAD(P) is an indispensable cofactor for all organisms and its biosynthetic pathways are proposed as promising novel antibiotics targets against pathogens such as Mycobacterium tuberculosis. Six NAD(P) biosynthetic pathways were reconstructed by comparative genomics: de novo pathway (Asp), de novo pathway (Try), NmR pathway I (RNK‐dependent), NmR pathway II (RNK‐independent), Niacin salvage, and Niacin recycling. Three enzymes pivotal to the key reactions of NAD(P) biosynthesis are shared by almost all organisms, that is, NMN/NaMN adenylyltransferase (NMN/NaMNAT), NAD synthetase (NADS), and NAD kinase (NADK). They might serve as ideal broad spectrum antibiotic targets. Studies in M. tuberculosis have in part tested such hypothesis. Three regulatory factors NadR, NiaR, and NrtR, which regulate NAD biosynthesis, have been identified. M. tuberculosis NAD(P) metabolism and regulation thereof, potential drug targets and drug development are summarized in this paper. J. Cell. Physiol. 226: 331–340, 2011. © 2010 Wiley‐Liss, Inc.
Voltage‐gated K+ channels play a role in cAMP‐stimulated neuritogenesis in mouse neuroblastoma N2A cells
Authors: Leung Yuk‐Man , Huang Chien‐Fang , Chao Chia‐Chia , Lu Dah‐Yuu , Kuo Chang‐Shin , Cheng Tzu‐Hurng , Chang Li‐Yun , Chou Chun‐Hsiao 
Published: September 2010

AbstractNeuritogenesis is essential in establishing the neuronal circuitry. An important intracellular signal causing neuritogenesis is cAMP. In this report, we showed that an increase in intracellular cAMP stimulated neuritogenesis in neuroblastoma N2A cells via a PKA‐dependent pathway. Two voltage‐gated K+ (Kv) channel blockers, 4‐aminopyridine (4‐AP) and tetraethylammonium (TEA), inhibited cAMP‐stimulated neuritogenesis in N2A cells in a concentration‐dependent manner that remarkably matched their ability to inhibit Kv currents in these cells. Consistently, siRNA knock down of Kv1.1, Kv1.4, and Kv2.1 expression reduced Kv currents and inhibited cAMP‐stimulated neuritogenesis. Kv1.1, Kv1.4, and Kv2.1 channels were expressed in the cell bodies and neurites as shown by immunohistochemistry. Microfluorimetric imaging of intracellular [K+] demonstrated that [K+] in neurites was lower than that in the cell body. We also showed that cAMP‐stimulated neuritogenesis may not involve voltage‐gated Ca2+ or Na+ channels. Taken together, the results suggest a role of Kv channels and enhanced K+ efflux in cAMP/PKA‐stimulated neuritogenesis in N2A cells. J. Cell. Physiol. 226: 1090–1098, 2011. © 2010 Wiley‐Liss, Inc.
Accumulation of protein carbonyls within cerebellar astrocytes in murine experimental autoimmune encephalomyelitis
Authors: Zheng Jianzheng , Bizzozero Oscar A. 
Published: September 2010

AbstractRecent work from our laboratory has implicated protein carbonylation in the pathophysiology of multiple sclerosis (MS) and experimental autoimmune encephalomyelitis (EAE). The present study was designed to determine the changes in protein carbonylation during disease progression and to identify the target cells and modified proteins in the cerebellum of EAE animals, prepared by active immunization of C57/BL6 mice with MOG35–55 peptide. In this model, protein carbonylation was maximal at the peak of the disease (acute phase), to decrease thereafter (chronic phase). Double‐immunofluorescence microscopy of affected cerebella showed that carbonyls accumulate in white matter astrocytes and to a lesser extent in microglia/macrophages, in both the acute and the chronic phase. Surprisingly, T cells, oligodendrocytes, and neurons were barely stained. By 2D oxyblot and mass spectrometry, β‐actin, β‐tubulin, GFAP, and HSC‐71 were identified as the major targets of carbonylation throughout the disease. Using a pull‐down/Western blot method, we found a significant increase in the proportion of carbonylated β‐actin, β‐tubulin, and GFAP in the chronic phase but not in the acute phase. These results suggest that as disease progresses from the inflammatory to the neurodegenerative phase there may be an inappropriate removal of oxidized cytoskeletal proteins. Additionally, the extensive accumulation of carbonylated GFAP in the chronic phase of EAE may be responsible for the abnormal shape of astrocytes observed at this stage. © 2010 Wiley‐Liss, Inc.
Homozygous inactivation of theLGI1gene results in hypomyelination in the peripheral and central nervous systems
Authors: Silva Jeane , Sharma Suash , Hughes Bernard , Yu Y. Eugene , Cowell John K. 
Published: September 2010

AbstractMutations in theLGI1gene in humans predispose to the development of autosomal dominant partial epilepsy with auditory features (ADPEAF). Homozygous inactivation of theLgi1gene in mice results in an epilepsy phenotype characterized by clonic seizures within 2–3 weeks after birth. Before onset of seizures, the 2–3‐week‐old null mutant mice show poor locomotor activity and neuromuscular strength. EM analysis of the sciatic nerve demonstrates impaired myelination of axons in the peripheral nervous system. Although heterozygous mutant mice do not show any locomotor phenotypes, they also demonstrate an intermediate level of hypomyelination compared with the wild‐type mice. Hypomyelination was also observed in the central nervous system, which, although relatively mild, was still significantly different from that of the wild‐type mice. These data suggest a role for LGI1 in the myelination functions of Schwann cells and oligodendrocytes. © 2010 Wiley‐Liss, Inc.