bluuebunny/crossref_metadata_2025_split huggingface dataset:
A single infusion of zoledronic acid produces sustained remissions in paget disease: Data to 6.5 years
Authors: Reid Ian R , Lyles Kenneth , Su Guoqin , Brown Jacques P , Walsh John P , del Pino-Montes Javier , Miller Paul D , Fraser William D , Cafoncelli Susan , Bucci-Rechtweg Christina , Hosking David J 
Published: June 2011

Abstract
Two trials have shown that a single 5-mg infusion of zoledronic acid achieves much higher response rates in Paget disease of bone than risedronate. The duration of this effect is unknown. We have conducted an open follow-up of responders from the two trials (152 originally treated with zoledronic acid, 115 with risedronate) out to 6.5 years without further intervention. Endpoints were times to relapse (ie, return of serum total alkaline phosphatase activity to within 20% of the pretreatment value) or loss of response (response = normalization of alkaline phosphatase or 75% or greater reduction in its excess). Bone turnover markers were lower in the zoledronic acid group throughout follow-up, with mean alkaline phosphatase (ALP) remaining within the reference range in these patients, whereas the mean in the risedronate group was above normal from 1 year. Relapse rates were substantially greater in the risedronate group (23 of 115, 20%) than in those treated with zoledronic acid (1 of 152, 0.7%, p &lt; .001), and loss of response occurred in 19 (12.5%) zoledronic acid patients compared with 71 (62%) risedronate patients (p &lt; .0001). Risk ratios for relapse and loss of response in zoledronic acid patients were 0.02 [95% confidence interval (CI) 0.00–0.18] and 0.12 (95% CI 0.07–0.19), respectively. Changes from baseline in quality of life, assessed using SF-36 scores, were more positive in the zoledronic acid group across the follow-up period (p = .01). Bone markers at 6 months were predictive of response duration. These data demonstrate an unprecedented duration of remission of Paget disease following treatment with zoledronic acid, accompanied by an improved quality of life. © 2011 American Society for Bone and Mineral Research
Aromatase is expressed and active in the rainbow trout oocyte during final oocyte maturation
Authors: Gohin Maella , Bodinier Pascal , Fostier Alexis , Chesnel Franck , Bobe Julien 
Published: June 2011

AbstractWhile it is generally well accepted that the ovarian follicular sites of estradiol‐17β (E2) synthesis are restricted to somatic cells, the possible contribution of the germinal compartment has received little or no attention in teleosts. In order to demonstrate the expression of ovarian aromatase in the oocyte, cyp19a1a mRNA was studied in ovarian follicles by in situ hybridization. In addition, the expression of cyp19a1a was studied in both somatic and germinal compartments of the ovarian follicle in rainbow trout (Oncorhynchus mykiss) during final oocyte maturation (i.e., maturational competence acquisition and subsequent meiosis resumption) by real‐time PCR. The enzymatic activity of ovarian aromatase was also studied in both somatic and germinal compartments of the ovarian follicle. Finally, E2 levels were monitored in follicle‐enclosed oocytes throughout the pre‐ovulatory period. We were able to demonstrate a significant ovarian aromatase expression and activity in the late vitellogenic oocyte. Furthermore, a dramatic decrease in aromatase expression and activity occurs in the oocyte during late oogenesis, concomitantly with the trend observed in surrounding follicular layers. We also report an unexpected increase of E2 levels in the oocyte during the pre‐ovulatory period. To our knowledge, these observations are reported for the first time in any teleost species. Together, our data support the hypothesis of the participation of the germinal compartment in follicular estrogen synthesis and a biological role of E2 during oocyte and/or early embryo development. Mol. Reprod. Dev. 78:510–518, 2011. © 2011 Wiley‐Liss, Inc.
Improved in vitro development of cloned bovine embryos using S‐adenosylhomocysteine, a non‐toxic epigenetic modifying reagent
Authors: Jafari Shahram , Hosseini Morteza S. , Hajian Mahdi , Forouzanfar Mohsen , Jafarpour Farnoosh , Abedi Parvaneh , Ostadhosseini Somayyeh , Abbasi Hassan , Gourabi Hamid , Shahverdi Abdol H. , Dizaj Ahmad Vosough Taghi , Anjomshoaa Maryam , Haron Wahid , Noorshariza Noor , Yakub Halimhatoon , Nasr‐Esfahani Mohammad H. 
Published: June 2011

AbstractIn this study, fibroblast cells were stably transfected with mouse POU5F1 promoter‐driven enhanced green fluorescent protein (EGFP) to investigate the effect of S‐adenosylhomocysteine (SAH), the reversible non‐toxic inhibitor of DNA‐methyltransferases (DNMTs), at different intervals post‐fusion on in vitro development of cloned bovine embryos. Treatment with SAH for 12 hr resulted in 54.6 ± 7.7% blastocyst production, which was significantly greater than in vitro fertilized embryos (IVF: 37.2 ± 2.7%), cloned embryos treated with SAH for 72 hr (31.0 ± 7.6%), and control cloned embryos (34.6 ± 3.6%). The fluorescence intensities of the EGFP‐POU5F1 reporter gene at all intervals of SAH treatment, except of 72 hr, were significantly higher than control somatic cell nuclear transfers (SCNT) embryos. The intensity of DNA‐methylation in cloned embryos treated with SAH for 48 hr was similar to that of IVF embryos, and was significantly lower than the other SCNT groups. The levels of H3K9 acetylation in all SCNT groups were significantly lower than IVF embryos. Real‐time PCR analysis of gene expression revealed significantly higher expression of POU5F1 in cloned versus IVF blastocysts. Neither embryo production method (SCNT vs. IVF) nor the SAH treatment interval affected expression of the BCL2 gene. Cloned embryos at all intervals of SAH treatment, except for 24 hr, had significantly increased VEGF transcript compared to IVF and control SCNT embryos. It was suggested that the time interval of DNMT inhibition may have important consequences on different in vitro features of bovine SCNT, and the improving effects of DNMT inhibition on developmental competency of cloned embryos are restricted to a specific period of time preceding de novo methylation. Mol. Reprod. Dev. 78:576–584, 2011. © 2011 Wiley‐Liss, Inc.
Attachment style and schema as predictors of social functioning in youth with borderline features
Authors: Hulbert Carol A. , Jennings Tarni C. , Jackson Henry J. , Chanen Andrew M. 
Published: June 2011

ABSTRACTTheorized associations between adverse attachment experience, maladaptive core schema (EMS) and the interpersonal dysfunction seen as characteristic of borderline personality disorder were investigated in two groups of outpatient youth aged between 15 and 25 years. Patients with sub‐syndromal (meeting at least 3 DSM‐IV criteria) and full syndrome BPD (n = 30) and patients with major depressive disorder completed self‐report measures of adult attachment style, EMS and interpersonal functioning. Results for both groups indicated a level of disadvantage for each of the domains assessed. Contrary to prediction, mean scores for the two groups did not differ for anxious or avoidant attachment style or for 13 or the 15 EMS assessed. BPD participants scored at a significantly higher level for the Mistrust/abuse and Entitlement schema. Consistent with results reported for adult samples, the BDP group acknowledged a predominantly anxious attachment style, a wide range of EMS and poor interpersonal functioning. Hierarchical multiple regression analyses undertaken for each EMS domain in turn indicated that an anxious attachment style and endorsement of the Social Isolation EMS were the strongest predictors of social functioning. Against, prediction, there was limited evidence supporting the specificity of theorized relationships between BPD diagnosis and attachment style and prominence of Disconnection and Rejection domain EMS. Copyright © 2011 John Wiley & Sons, Ltd.
Evaluation and optimization of IgY Spin Column technology in the depletion of abundant proteins from human serum
Authors: Corrigan Laura , Jefferies Caroline , Clive Lee Thomas , Daly Jacqueline 
Published: June 2011

AbstractSerum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1‐D SDS‐PAGE and 2‐DE revealed a suboptimal performance of the IgY spin columns. However, modification of the manufacturer's protocol, subjecting samples to two rounds of depletion, improved the number of proteins resolved by 2‐DE. With alteration of the manufacturer protocol, the Seppro® IgY14 spin column can produce depleted serum with an increased number of spots resolved by 2‐DE compared to untreated serum.
Immune proteomics of Staphylococcus aureus
Authors: Bröker Barbara M. , van Belkum Alex 
Published: June 2011

AbstractImmune proteomics is an increasingly powerful tool for the investigation of the adaptive immune response to natural encounters between micro‐organisms and their hosts. The versatile species Staphylococcus aureus serves to illustrate how these techniques can be employed to appreciate the complexity and diversity of the host–pathogen interactions in unprecedented detail and completeness. Such knowledge is important for the development of effective vaccines as well as informative diagnostic and novel therapeutic tools. From high‐resolution immune proteome studies, general rules underlying the human adaptive immune response to S. aureus colonization and infection are beginning to emerge against a background of extreme diversity: S. aureus carriers develop immune memory for their colonizing strain, but even non‐carriers are frequently exposed to S. aureus, resulting in specific antibodies. During bacterial invasion, immune‐competent individuals rapidly mount an antibody response to a large panel of S. aureus antigens. However, every patient starts from a personal baseline antibody profile reflecting his or her history of encounters with S. aureus.
Site‐specific analysis of bacterial phosphoproteomes
Authors: Macek Boris , Mijakovic Ivan 
Published: June 2011

AbstractProtein phosphorylation on serine, threonine and tyrosine is established as an important regulatory modification in bacteria. A growing number of studies employing mass spectrometry‐based proteomics report large protein phosphorylation datasets, providing precise evidence for in‐vivo phosphorylation that is especially suitable for functional follow‐up. Here, we provide an overview of the strategies currently used in bacterial phosphoproteomics, with an emphasis on gel‐free proteomics and approaches that enable global detection of phosphorylation sites in bacterial proteins. The proteomics technology has matured sufficiently to permit routine characterization of phosphoproteomes and phosphopeptides with high sensitivity; we argue that the next challenge in the field will be the large‐scale detection of protein kinase and phosphatase substrates and their integration into regulatory networks of the bacterial cell.
Structural analysis of the N‐terminal fragment of the antiangiogenic protein endostatin: A molecular dynamics study
Authors: Monteiro Torres Pedro Henrique , Limaverde Soares Costa Sousa Gabriel , Pascutti Pedro Geraldo 
Published: June 2011

AbstractEndostatin is a potent antiangiogenic protein derived from the noncollagenous domain 1 (NC1) of collagen XVIII. The mechanism by which endostatin exerts its antiangiogenic effect is still incompletely understood. It has been shown that the 27 amino acid N‐terminal fragment of murine endostatin has antitumor, antimigration, and antipermeability activities comparable to the full soluble protein. To understand how this peptide can exert such elaborate function, we performed structural analysis using molecular dynamics to evaluate the behavior of this fragment in aqueous environment. Here, we show that the N‐terminal peptide of murine endostatin is able to assume a well‐defined structure, folding into a zinc‐dependent β‐hairpin conformation. Analyzing the folding mechanism, we were able to understand why the N‐terminal peptide of human endostatin with the same length failed to acquire a stable conformation. Conversely, we were able to predict the successful folding of the R4Q mutant and of a shorter form of the human peptide with 25 residues. Finally, we show that the β‐hairpin conformation assumed by the zinc‐bound peptide of murine endostatin has a high structural similarity with fragments of another family of angiogenesis inhibitors: the integrin‐binding portion of the NC1 domain of collagen IV. Indeed, our docking simulations show that arresten, canstatin, and the endostatin peptide bind to the same spot of αVβ3 integrin, suggesting similar interactions via a common binding site on this receptor. Proteins 2011;. © 2011 Wiley‐Liss, Inc.
On the cut‐off phenomenon for the transitivity of randomly generated subgroups
Authors: Galligo André , Miclo Laurent 
Published: June 2011

AbstractConsider K ≥ 2 independent copies of the random walk on the symmetric group SN starting from the identity and generated by the products of either independent uniform transpositions or independent uniform neighbor transpositions. At any time
\documentclass{article} \usepackage{amsmath,amsfonts}\pagestyle{empty}\begin{document} $n\in \mathbb{N}$ \end{document}, let Gn be the subgroup of SN generated by the K positions of the chains. In the uniform transposition model, we prove that there is a cut‐off phenomenon at time N ln(N)/(2K) for the non‐existence of fixed point of Gn and for the transitivity of Gn, thus showing that these properties occur before the chains have reached equilibrium. In the uniform neighbor transposition model, a transition for the non‐existence of a fixed point of Gn appears at time of order \documentclass{article} \usepackage{amsmath,amsfonts}\pagestyle{empty}\begin{document} $N^{1+\frac{2}{K}}$ \end{document}
(at least for K ≥ 3), but there is no cut‐off phenomenon. In the latter model, we recover a cut‐off phenomenon for the non‐existence of a fixed point at a time proportional to N by allowing the number K to be proportional to ln(N). The main tools of the proofs are spectral analysis and coupling techniques. © 2011 Wiley Periodicals, Inc. Random Struct. Alg., 2012
Expression pattern of Mical‐1 in the temporal neocortex of patients with intractable temporal epilepsy and pilocarpine‐induced rat model
Authors: Luo Jing , Xu Yali , Zhu Qiong , Zhao Fenghua , Zhang Ying , Peng Xi , Wang Wei , Wang Xuefeng 
Published: June 2011

AbstractMical‐1 is a novel F‐actin‐disassembly factor that is critical in actin reorganization. It provides a molecular conduit through which actin reorganizes‐a hallmark of cell morphological changes, including axon navigation. However, whether Mical‐1 is involved in the epileptogenesis remains unknown. Here, we investigate Mical‐1 expression pattern in patients with intractable temporal lobe epilepsy (TLE) and pilocarpine‐induced rat model. We used double‐labeled immunoflurescence, immunohistochemistry, and Western blotting to assess the location and expression of Mical‐1 in temporal neocortex of patients with intractable TLE, and the expression pattern of Mical‐1 at different time point in the hippocampus and temporal lobe cortex of the pilocarpine‐induced rat model. Double‐labeled immunofluorescence showed that Mical‐1 was coexpressed with neuron‐specific enolase (NSE) in the cytoplasm of neurons in temporal neocortex of patients with TLE and hippocampus of rat model. Faint and scattered immunoreactivity for Mical‐1 in the neuron of temporal neocortex in TLE group, but strong immunoreactivity for Mical‐1 was shown in control subjects. To quantitatively evaluate the Mical‐1 immunoreactivity, we measured the mean optical density (OD) of Mical‐1. In the hippocampus of pilocarpine‐induced rat model, the OD values transient increased at 6 h after seizure then decreased from 1 day to 14 days, and returned to a subnormal level at 60 days. The lowest level of Mical‐1 expression occurred at 14 days after seizure in the hippocampus. In the temporal lobe cortex of rat model, the OD values decreased at all time point after kindling compared to the normal group. Furthermore, our Western blot analysis confirmed these expression patterns of Mical‐1 from latent stage to chronic stage. Our results indicate that in patients with TLE and pilocarpine‐induced rat model, the expression of Mical‐1 were followed a downtrend from the latent stage to chronic stage after seizure evoke. Thus, as an effect factor participated in F‐actin disassemble, Mical‐1 may associate with inner pathophysiological modulation in epilepsy. Synapse, 2011. © 2011 Wiley‐Liss, Inc.
Wellness as Fairness
Authors: Prilleltensky Isaac 
Published: June 2011

AbstractI argue that distinct conditions of justice lead to diverse wellness outcomes through a series of psychosocial processes. Optimal conditions of justice, suboptimal conditions of justice, vulnerable conditions of injustice, and persisting conditions of injustice lead to thriving, coping, confronting, and suffering, respectively. The processes that mediate between optimal conditions of justice and thriving include the promotion of responsive conditions, the prevention of threats, individual pursuit, and avoidance of comparisons. The mechanisms that mediate between suboptimal conditions of justice and coping include resilience, adaptation, compensation, and downward comparisons. Critical experiences, critical consciousness, critical action, and righteous comparisons mediate between vulnerable conditions of injustice and confrontation with the system. Oppression, internalization, helplessness, and upward comparisons mediate between persisting conditions of injustice and suffering. These psychosocial processes operate within and across personal, interpersonal, organizational and community contexts. Different types of justice are hypothesized to influence well‐being within each context. Intrapersonal injustice operates at the personal level, whereas distributive, procedural, relational, and developmental justice impact interpersonal well‐being. At the organizational level, distributive, procedural, relational and informational justice influence well‐being. Finally, at the community level, distributive, procedural, retributive, and cultural justice support community wellness. Data from a variety of sources support the suggested connections between justice and well‐being.
Emergency ultrasound diagnosis of a left atrial thrombus and diffuse emboli
Authors: Datta Anita , Kapoor Monica , Pearl-Davis Michelle , Lema Penelope Chun 
Published: June 2011

Abstract

Introduction
A 71-year-old female with a history of atrial fibrillation presented to the emergency department (ED) for abdominal pain. A computed tomography (CT) scan in ED showed an abdominal aortic thrombus and diffuse emboli suspicious for a cardiac source.

Methods
A bedside ultrasound was performed which confirmed the presence of a dilated left atrium with thrombus. The patient was admitted to the hospital and eventually placed on anticoagulation.

Conclusions
Atrial fibrillation is a common cause of atrial thrombus. Intracardiac thrombus can be diagnosed by bedside echocardiography in the ED. Emboli can occur as a complication of thrombus in the atrium. Management may include anticoagulation or surgical intervention.
Brief report: Gum chewing affects standardized math scores in adolescents
Authors: Johnston Craig A. , Tyler Chermaine , Stansberry Sandra A. , Moreno Jennette P. , Foreyt John P. 
Published: June 2011

ABSTRACTGum chewing has been shown to improve cognitive performance in adults; however, gum chewing has not been evaluated in children. This study examined the effects of gum chewing on standardized test scores and class grades of eighth grade math students. Math classes were randomized to a gum chewing (GC) condition that provided students with gum during class and testing, or a control condition with no gum (NGC). Participants included 108 students. The math sections of the Texas Assessment of Knowledge and Skills (TAKS) and the Woodcock Johnson III Tests of Achievement (WJ‐III), and math class grades were used to assess academic performance. Students in the gum chewing condition improved standardized test scores and maintained higher grades in math class compared to those in the no‐gum chewing condition. These results are encouraging as gum chewing may be a cost‐effective and easily implemented method to increase student performance.Trial Registration: NCT00792116.
Effects of partial rootzone drying and rootstock vigour on dry matter partitioning of apple trees (Malus domesticacvar Pink Lady)
Authors: LO BIANCO R. , TALLUTO G. , FARINA V. 
Published: June 2011

SUMMARYThe effects of partial rootzone drying (PRD) and rootstock vigour on dry matter accumulation and partitioning among leaves, shoots, fruits, frame and roots of apple trees (Malus domesticaBorkh. cvar Pink Lady) were investigated in 2005 near Caltavuturo, in Sicily. In a first field trial, trees on MM.106 rootstock were subjected to: conventional irrigation (CI), maintaining soil moisture above 0·80 of field capacity; PRD irrigation, where alternating sides of the rootzone received 0·50 of the CI irrigation water; and continuous deficit irrigation (DI), where 0·50 of the CI water was equally applied to both sides of the rootzone. In a second trial, trees on M.9 or MM.106 were subjected to CI and PRD irrigation. In trial 1, dry matter accumulation was markedly reduced by DI irrigation and to a lesser extent by PRD; PRD trees partitioned 20% less to leaves, 31% less to fruits and 24% more to woody components than CI trees; DI trees partitioned 14% less to current shoots and 18% more to fruits than CI and had the highest fruit:leaf ratio. In trial 2, there was no interaction between rootstock and irrigation treatments. MM.106 induced greater leaf, shoot, frame and root dry weights (DWs) than M.9, resulting in more vegetative growth and larger trees. PRD reduced leaf, shoot, frame and fruit DWs, while root DWs were similar to CI, and thus PRD trees were 18% smaller than CI trees. Neither rootstock nor irrigation affected dry matter partitioning among organs or root:canopy ratio, whereas PRD trees or trees on MM.106 showed better water use efficiency than CI and M.9, respectively. The results show that PRD trees did not activate drought tolerance strategies in terms of dry matter allocation that could improve acquisition of water resources, regardless of rootstock. PRD irrigation increased above-ground dry matter partitioning towards woody components at the expense of leaves and fruits.