bluuebunny/crossref_metadata_2025_split huggingface dataset:
A MAGE‐C2 antigenic peptide processed by the immunoproteasome is recognized by cytolytic T cells isolated from a melanoma patient after successful immunotherapy
Authors: Ma Wenbin , Vigneron Nathalie , Chapiro Jacques , Stroobant Vincent , Germeau Catherine , Boon Thierry , Coulie Pierre G. , Van den Eynde Benoît J. 
Published: January 2011

AbstractWe have pursued our analysis of a melanoma patient who showed almost complete tumor regression following vaccination with MAGE‐A1 and MAGE‐A3 antigens. We previously described high frequencies of tumor‐specific CTL precursors in blood samples collected after but also before vaccination. A set of CTL clones were derived that recognized antigens different from those of the vaccine. Two of these antigens were peptides encoded by another MAGE gene, MAGE‐C2. Here we describe the antigen recognized by another tumor‐specific CTL clone. It proved to be a third antigenic peptide encoded by gene MAGE‐C2, ASSTLYLVF. It is presented by HLA‐B57 molecules and proteasome‐dependent. Tumor cells exposed to interferon‐gamma (IFN‐γ) were better recognized by the anti‐MAGE‐C242‐50 CTL clone. This mainly resulted from a better processing of the peptide by the immunoproteasome as compared to the standard proteasome. Mass spectrometric analyses showed that the latter destroyed the antigenic peptide by cleaving between two internal hydrophobic residues. Despite its higher “chymotryptic‐like” (posthydrophobic) activity, the immunoproteasome did not cleave at this position, in line with the suggestion that hydrophobic residues immediately downstream from a cleavage site impair cleavage by the immunoproteasome. We previously reported that one of the other MAGE‐C2 peptides recognized by CTL from this patient was also better processed by the immunoproteasome. Together, these results support the notion that the tumor regression of this patient was mediated by an antitumor response shaped by IFN‐γ and dominated by CTL directed against peptides that are better produced by the immunoproteasome, such as the MAGE‐C2 peptides.
The association between Parkinson's disease and melanoma
Authors: Pan Tianhong , Li Xinqun , Jankovic Joseph 
Published: January 2011

AbstractParkinson's disease (PD) is a neurodegenerative disorder characterized by a loss of melanin‐positive, dopaminergic neurons in the substantia nigra. Although there is convincing epidemiologic evidence of a negative association between PD and most cancers, a notable exception to this is that melanoma, a malignant tumor of melanin‐producing cells in skin, occurs with higher‐than‐expected frequency among subjects with PD and that melanoma patients are more likely to have PD. A clear biological explanation for this epidemiological observation is lacking. Here, we present a comprehensive review of published literature exploring the association between PD and melanoma. On the basis of published findings, we conclude that (i) changes in pigmentation including melanin synthesis and/or melanin synthesis enzymes, such as tyrosinase and tyrosine hydroxylase, play important roles in altered vulnerability for both PD and melanoma; (ii) changes of PD‐related genes such asParkin,LRRK2and α‐synucleinmay increase the risk of melanoma; (iii) changes in some low‐penetrance genes such as cytochrome p450 debrisoquine hydroxylase locus, glutathioneS‐transferase M1 and vitamin D receptor could increase the risk for both PD and melanoma and (iv) impaired autophagy in both PD and melanoma could also explain the association between PD and melanoma. Future studies are required to address whether altered pigmentation, PD‐ or melanoma‐related gene changes and/or changes in autophagy function induce oncogenesis or apoptosis. From a clinical point of view, early diagnosis of melanoma in PD patients is critical and can be enhanced by periodic dermatological surveillance, including skin biopsies.
Neonatal UVB exposure accelerates melanoma growth and enhances distant metastases in Hgf‐Cdk4R24C C57BL/6 mice
Authors: Gaffal Evelyn , Landsberg Jennifer , Bald Tobias , Sporleder Alexander , Kohlmeyer Judith , Tüting Thomas 
Published: January 2011

AbstractGenetically engineered mouse models offer new opportunities to experimentally investigate the impact of UV on melanoma pathogenesis. Here we irradiated a cohort of newborn 15 Hgf‐Cdk4R24C mice on the pigmented C57BL/6 background with one erythemogenic dose of 6 kJ/m2 UVB and compared the development of nevi and melanoma with a cohort of 30 untreated Hgf‐Cdk4R24C mice. Neonatal UVB exposure decreased the latency and accelerated the growth of primary melanomas resulting in a significantly decreased time from melanoma onset to melanoma‐related death (61 days vs. 96 days). Interestingly, we did not observe differences in the development of melanocytic nevi. Histopathological investigations revealed that UVB irradiation shifted the spectrum of melanomas toward a more aggressive phenotype with increased tumor cell proliferation, invasive growth and enhanced angiogenesis. Accordingly, we observed distal melanoma metastases in the lungs more frequently in the UV‐irradiated than in the untreated cohort of Hgf‐Cdk4R24C mice (73% vs. 47%). UVB‐induced melanomas only contained very few infiltrating immune cells and expressed very low levels of proinflammatory chemokines. Taken together, our results demonstrate that neonatal UVB exposure promoted the early appearance of rapidly enlarging primary melanomas in Hgf‐Cdk4R24C C57BL/6 mice which showed enhanced invasive and metastatic behaviour without a persistent tumor‐associated inflammatory response. The preferential impact of UVB irradiation on the progression of melanoma without an effect on the development of nevi supports the hypothesis that the molecular targets of UVB are involved in bypassing the proliferative arrest of transformed melanocytes without alerting a cellular immune response.
Kinesin 18A expression: Clinical relevance to colorectal cancer progression
Authors: Nagahara Makoto , Nishida Naohiro , Iwatsuki Masaaki , Ishimaru Shinya , Mimori Koshi , Tanaka Fumiaki , Nakagawa Tsuyoshi , Sato Takanobu , Sugihara Kenichi , Hoon Dave S.B. , Mori Masaki 
Published: January 2011

AbstractKif18A, a member of the kinesin superfamily of molecular motor proteins, is a microtubule depolymerase and a key regulator of chromosome congregation. Kif18A's role in cancer progression has not been well defined. Our hypothesis is that Kif18A has a role in the progression of colorectal cancer (CRC). To investigate this expression of Kif18A, mRNA was assessed by quantitative real‐time PCR in 113 operative specimens of primary CRC. Kif18A was overexpressed and significantly (p < 0.0001) higher in CRC than in normal colon tissue. Kif18A overexpression in CRC significantly correlated with clinicopathologic factors such as tumor stage (p < 0.0001), lymphatic invasion (p = 0.001), lymph node metastasis (p = 0.01), venous invasion (p = 0.002) and peritoneal dissemination (p = 0.02), suggesting that it has a key role in CRC progression. In multivariate analysis, high Kif18A expression had independent significance for poorer overall survival after resection of CRC (p = 0.037). To demonstrate Kif18A's role in CRC progression, we performed translational and in situ studies. Using in vitro studies on CRC cell lines, we evaluated Kif18A's role in proliferation, migration and invasion. CRC cells transfected with Kif18A cDNA demonstrated significant enhanced migration (p < 0.01) and invasion (p = 0.018) compared to mock‐transfected cells. When Kif18A was targeted with specific small interfering RNA, CRC cells had significantly reduced proliferation (p < 0.01), migration (p < 0.01) and invasion (p < 0.05). The in vitro and translational studies demonstrated that Kif18A expression is related to events of metastasis and is a significant factor for CRC progression.
Chromatin‐modifying drugs induce miRNA‐153 expression to suppress Irs‐2 in glioblastoma cell lines
Authors: Xu Jianzhen , Liao Xuemei , Lu Na , Liu Wenming , Wong Chi‐Wai 
Published: January 2011

AbstractMicroRNAs (miRNAs) are noncoding RNAs that regulate gene expression by inhibiting translation or by promoting mRNA degradation. Previously, we established that microRNA‐153 (miR‐153) induces apoptosis by downregulating B‐cell lymphoma 2 (Bcl‐2) and myeloid cell leukemia sequence 1 (Mcl‐1) protein expression levels in glioblastoma cell line DBTRG‐05MG. In our study, we show that ectopic expression of miR‐153 also inhibits the protein kinase B (PKB/Akt) pathway via reducing the protein level of insulin receptor substrate‐2 (Irs‐2). Moreover, simultaneous treatment with the chromatin‐modifying drugs 4‐phenylbutyric acid and 5‐aza‐2′‐deoxycytidine induces miR‐153 expression to suppress Irs‐2, Bcl‐2 and Mcl‐1 expressions, thus downregulating the survival but upregulating the apoptotic pathways, implying that tumor suppressor miR‐153 is a dual life and death regulator.
Estimating Engel curves under unit and item nonresponse
Authors: De Luca Giuseppe , Peracchi Franco 
Published: January 2011

SUMMARYThis paper estimates food Engel curves using data from the first wave of the Survey on Health, Aging and Retirement in Europe (SHARE). Our statistical model simultaneously takes into account selectivity due to unit and item nonresponse, endogeneity problems, and issues related to flexible specification of the relationship of interest. We estimate both parametric and semiparametric specifications of the model. The parametric specification assumes that the unobservables in the model follow a multivariate Gaussian distribution, while the semiparametric specification avoids distributional assumptions about the unobservables. Copyright © 2011 John Wiley & Sons, Ltd.
Angiogenesis and osteogenesis enhanced by bFGFex vivogene therapy for bone tissue engineering in reconstruction of calvarial defects
Authors: Qu Dan , Li Jihua , Li Yubao , Gao Ying , Zuo Yi , Hsu Yuchun , Hu Jing 
Published: January 2011

AbstractReconstruction of bone defects by tissue engineered substitutes requires coordinated coupling between osteogenesis and angiogenesis. Basic fibroblast growth factor (bFGF or FGF‐2) is a protein which acts actively in osteogenesis and angiogenesis during skeletal healing and development. It is hypothesized that BMSCs transfected with bFGF can directly stimulate regeneration of vascular tissue, and subsequently enhance osseous formation and remodeling after implantation of the tissue engineered bone. This study was designed to examine the impact of bFGF‐BMSCs, seeded on nano‐hydroxyapatite/polyamide66 (n‐HA/PA66) composite scaffold, to enhance angiogenesis and osteogenesis in a calvarial critical‐sized defect model in rats. To investigate the vascularization and bone formation of tissue engineered bone, the substrate was removed and processed for immunohistochemical, scanning electron microscopic examinations (SEM), reverse transcriptase‐polymerase chain reaction (RT‐PCR), dual energy X‐ray absorptiometry (DEXA), microvessels counting, and new bone volume assay. The results demonstrate that bFGF mediatedex vivogene transfer based on BMSCs can accelerate vascularization and bone regeneration on these composite scaffolds. The n‐HA/PA66 scaffold combined with the bFGF‐BMSCs may mimic the natural process of osteogenesis during repair of defect by tissue engineered bone. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
Predicting hematopoietic stem cell mobilization failure in patients with multiple myeloma: A simple method using day 1 CD34+ cell yield
Authors: Duong Hien K. , Bolwell Brian J. , Rybicki Lisa , Koo Anna , Hsi Eric D. , Figueroa Priscilla , Dean Robert , Pohlman Brad , Kalaycio Matt , Andresen Steven , Sobecks Ronald , Copelan Edward 
Published: January 2011

AbstractEarly and reliable prediction of the likelihood of achieving adequate stem cell collection for autologous stem cell transplantation (ASCT) in patients with multiple myeloma (MM) would improve collection efficiency, prevent unnecessary aphereses, and permit appropriate treatment alterations. No previous study has reported a threshold CD34+ cell collection quantity on Day 1 or 2 of leukapheresis that could predict successful stem cell collection. We performed a retrospective analysis of all MM patients undergoing first attempt of stem cell collection at our institution from 2001 through 2008. Recursive partitioning analysis was used to identify Day 1 or Day 1+2 CD34+ collection quantity that predicted failure to reach target ≥2 × 106 CD34+ cells/kg within five days of collection. Totally, 172 patients were included in the analysis. Patients underwent mobilization with G‐CSF or G‐CSF+ chemotherapy. 23 of 172 patients (13.4%) failed to collect sufficient (≥2 × 106 CD34+ cells/kg) CD34+ cells after five days of apheresis: 22 of 29 who collected ≤0.70 × 106 CD34+ cells/kg and 1 of 143 who collected >0.70 × 106 CD34+ cells/kg (75.9% vs. 0.7%, P < 0.001) on Day 1. Collection failure occurred in 23 of 30 patients who collected ≤1.54 × 106 CD34+ cells/kg and 0 of 142 who collected >1.54 × 106 CD34+ cells/kg (76.7% vs. 0%, P < 0.001) on Days 1 + 2. Day 1 CD34+ cell collection quantity identifies patients unlikely to achieve adequate collection for ASCT. Patients who collect ≤0.70 × 106 CD34+ cells/kg on day 1 could be considered for treatment modifications to improve CD34+ collection, such as early administration of plerixafor or large volume apheresis. J. Clin. Apheresis, 2011. © 2011 Wiley‐Liss, Inc.
Utilization of mixed monosaccharides for power generation in microbial fuel cells
Authors: Catal Tunc , Fan Yanzhen , Li Kaichang , Bermek Hakan , Liu Hong 
Published: January 2011

AbstractBACKGROUND: The utilization of mixed monosaccharides commonly found in the hydrolysates of lignocellulosic biomass was evaluated for power generation in single chamber air cathode mediator‐less microbial fuel cells.RESULTS: A similar voltage generation pattern was observed for all the MFCs with different monosaccharide combinations and an external resistance of 1000 Ω. However, the different monosaccharide utilization rates ranging from 212 mg L−1 h−1 to 389 mg L−1 h−1 indicate the presence of preferential utilization of different monosaccharides. Three volatile fatty acids (VFAs), including acetic, propionic and butyric acids were detected as the main intermediates, which were generated mainly through a fermentation process.CONCLUSION: VFAs produced from initial monosaccharides contributed to a significant portion of the total electricity generated, and the fermentation process outcompeted the electricity generation process when a mixed bacterial culture was used. Copyright © 2011 Society of Chemical Industry
Performance of a batch two‐chambered microbial fuel cell operated at different anode potentials
Authors: Wang Xin , Feng Yujie , Liu Jia , Lee He , Ren Nanqi 
Published: January 2011

AbstractBACKGROUND: For a microbial fuel cell (MFC), the anode potential is an important electrochemical parameter for the growth, electron distribution and electrical activity of exoelectrogenic bacteria.RESULTS: The performance of a batch‐flow two‐chambered MFC was investigated operated at anode potentials of − 400, − 200, 0, + 200 and + 400 mV versus Ag/AgCl reference electrode. Glucose was used as substrate and ferricyanide as cathodic electron acceptor. At potentials from − 400 to + 200 mV, increasing maximum current outputs were obtained, ranging from 6.0 to 8.6 mA. However, the maximum current output decreased to 6.9 mA when the anode potential was further increased to + 400 mV. More positive potentials resulted in higher Coulombic efficiencies (CEs), with values increased from 64% (−400 mV) to 88% (+400 mV). Electrons deposited as H2 and CH4 resulted in lower CEs at more negative potentials (−400, − 200 and 0 mV). When + 400 mV was applied to the anode, the growth yield dropped from 0.25 ± 0.05 to 0.11 mol biomass mol−1 COD (chemical oxygen demand) with inhibition of electrical activity.CONCLUSIONS: The most positive anode potential reduced growth yield and inhibited bacterial growth, but electrical activity increased for potentials ranged from − 400 to + 200 mV. When the potential was increased to + 400 mV, growth yield was limited, affecting MFC performance. Copyright © 2011 Society of Chemical Industry
Paulownia as a raw material for the production of pulp by soda–anthraquinone cooking with or without previous autohydrolysis
Authors: García Juan Carlos , Zamudio Minerva Ana Maria , Pérez Antonio , De Alva Hugo Eduardo , López Francisco 
Published: January 2011

AbstractBACKGROUND: A central composite experimental design and multiple regression were used to model the production of pulp and paper sheets from a Paulownia fortunei × tormentosa × elongata trihybrid clone. Also, the same experiments were carried out with the solid phase after autohydrolysis of the raw material.RESULTS: The results were compared with those obtained from the solid phase remaining after autohydrolysis of the raw material with a view to optimizing the use of hemicelluloses and cellulose by fractionation. Pulp and paper sheets from the trihybrid clone surpass those from other Paulownia species such as P. fortunei and P. elongata in physical properties such as tensile index (11.6–49.1 Nm g−1), burst index (0.23–2.41 MPa m2 kg−1), tear index (0.71–3.03 mN m2 g−1) and intrinsic viscosity (427–958 cm3 g−1).CONCLUSIONS: The solid phase obtained by autohydrolysis of the Paulownia trihybrid provides pulp and paper sheets comparable in strength‐related properties with those of pulp directly produced from the raw material, but using substantially milder operating conditions and with the added advantage that the autohydrolysis treatment provides highly valorizable liquor. Copyright © 2011 Society of Chemical Industry
Cell therapy for fibrotic interstitial pulmonary disease: Experimental study
Authors: Cabral Rosa M. , BRANCO ÉRIKA , Rizzo Márcia Dos S. , Ferreira Guilherme J. , Gregores Guilherme B. , Samoto Vivian Y. , Stopiglia Ângelo J. , Maiorka Paulo C. , Fioretto Emerson T. , Capelozzi Vera L. , Borges João B. , Gomes Susimeire , Beraldo Marcelo A. , Carvalho Carlos R. R. , Miglino Maria A. 
Published: January 2011

AbstractParte superior do formulário Digite um texto ou endereço de um site ou traduza um documento. The aim of this study is to evaluate the histological changes in lung parenchyma of pigs affected by interstitial lung disease induced after the infusion of bone marrow mononuclear cells (BMMCs). Ten female swines were submitted to pulmonary fibrosis induced by a single dose of intratracheal bleomicine sulfate. Animals were arranged into two groups: Group 1: induced‐disease control and Group 2: cell therapy using BMMCs. Both groups were clinically evaluated for 180 days. High‐resolution computed tomography (HRCT) was performed at 90 and 180 days. BMMC sampling was performed in cell therapy group at 90 days. Euthanasia was performed, and samples were collected for histology and immunohistochemistry. The 90‐days HRCT demonstrated typical interstitial lesions in pulmonary parenchyma similarly to human disease. The 180‐days HRCT in Group 1 demonstrated advanced stages of the disease when compared with Group 2. Immunohistochemistry analysis suggests the presence of pre‐existent vessels and neoformed vessels as well as predominant young cells in the injured parenchyma of Group 2. Immunohistochemistry analysis suggests that cell therapy would promote a reconstructive response. Histology and HRCT analysis suggest a positive application of swine as a model for a bleomicine inducing of fibrotic interstitial pulmonary disease. Microsc. Res. Tech., 2011. © 2011 Wiley‐Liss, Inc.
Nazism, philosophy, and academic accountability: The real controversy surrounding Emmanuel Faye's Heidegger
Authors: Gilbert‐Walsh James 
Published: January 2011

AbstractIn this essay review, I argue that Emmanuel Faye's Heidegger: The Introduction of Nazism into Philosophy is, as a work of scholarship, a disappointment: Though Faye claims to demonstrate important connections between Heidegger's National Socialist commitments and his philosophical work, Faye offers the reader close, careful analysis of neither. In short, the book fails to deliver on its promise. But I also argue that the wave of attention Faye's book has attracted since its English translation appeared is symptomatic of a broad set of problems plaguing contemporary Anglophone philosophy. © 2011 Wiley Periodicals, Inc.
Rapid idea of located defects on grounding systems
Authors: Moussa Lefouili , Kerroum Kamel , El Khamlichi Drissi Khalil 
Published: January 2011

AbstractThis study gives an original way to have a rapid idea of the position of the located defects on grounding systems. For determining electromagnetic fields' radiation, we use an easy method, based on analytical formula, transmission line theory, and modified images theory, instead of complex method of Summerfield's integrals. The cartography of the electromagnetic fields radiated at the soil—air interface, by energized grounding systems (electrode or grid), can be used to detect and localize a defect on these systems. This model is aimed at helping in electromagnetic compatibility and lightning protection studies. Copyright © 2011 John Wiley & Sons, Ltd.
Figures‐of‐merit genetic extraction for InGaAs lasers, SiGe low‐noise amplifiers, and ZnSe/Ge/GaAs HBTs
Authors: Tseng H. C. , Horng J. B. , Hu C. 
Published: January 2011

AbstractIn this paper, we have successfully developed an intellectual parameter‐extraction methodology on the basis of a genetic algorithm (GA), involving the efficient search‐space separation and local‐minima‐convergence prevention schemes. Via an evolutionary simulation tool complemented with appropriate analytic equations, the enhanced approach has been applied to determine the significant figures‐of‐merit (FoMs), including internal quantum efficiency (ηi) as well as transparency current density (Jtr) of semiconductor lasers, minimum noise figure (NFmin) as well as associated available gain (GA,assoc) of low‐noise amplifiers (LNAs), and DC as well as AC characteristics of heterojunction bipolar transistors (HBTs). For the first time, demonstrated FoM‐extraction results, which coincide well with the actually measured data, for state‐of‐the‐art InGaAs quantum‐well lasers, advanced SiGe LNAs, and abrupt ZnSe/Ge/GaAs HBTs are simultaneously presented to validate this multi‐parameter analysis and robust optimization. Copyright © 2011 John Wiley & Sons, Ltd.