bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coexpression of high‐voltage‐activated ion channels Kv3.4 and Cav1.2 in pioneer axons during pathfinding in the developing rat forebrain
Authors: Huang Chia‐Yi , Chu Dachen , Hwang Wei‐Chao , Tsaur Meei‐Ling 
Published: April 2012

AbstractPrecise axon pathfinding is crucial for establishment of the initial neuronal network during development. Pioneer axons navigate without the help of preexisting axons and pave the way for follower axons that project later. Voltage‐gated ion channels make up the intrinsic electrical activity of pioneer axons and regulate axon pathfinding. To elucidate which channel molecules are present in pioneer axons, immunohistochemical analysis was performed to examine 14 voltage‐gated ion channels (Kv1.1–Kv1.3, Kv3.1–Kv3.4, Kv4.3, Cav1.2, Cav1.3, Cav2.2, Nav1.2, Nav1.6, and Nav1.9) in nine axonal tracts in the developing rat forebrain, including the optic nerve, corpus callosum, corticofugal fibers, thalamocortical axons, lateral olfactory tract, hippocamposeptal projection, anterior commissure, hippocampal commissure, and medial longitudinal fasciculus. We found A‐type K+ channel Kv3.4 in both pioneer axons and early follower axons and L‐type Ca2+ channel Cav1.2 in pioneer axons and early and late follower axons. Spatially, Kv3.4 and Cav1.2 were colocalized with markers of pioneer neurons and pioneer axons, such as deleted in colorectal cancer (DCC), in most fiber tracts examined. Temporally, Kv3.4 and Cav1.2 were expressed abundantly in most fiber tracts during axon pathfinding but were downregulated beginning in synaptogenesis. By contrast, delayed rectifier Kv channels (e.g., Kv1.1) and Nav channels (e.g., Nav1.2) were absent from these fiber tracts (except for the corpus callosum) during pathfinding of pioneer axons. These data suggest that Kv3.4 and Cav1.2, two high‐voltage‐activated ion channels, may act together to control Ca2+‐dependent electrical activity of pioneer axons and play important roles during axon pathfinding. J. Comp. Neurol. 520:3650–3672, 2012. © 2012 Wiley Periodicals, Inc.
Extended survival of SH‐SY5Y cells following overexpression of Lys67Glu neuroglobin is associated with stabilization of ΔψM
Authors: Skommer Joanna , Brittain Thomas 
Published: April 2012

AbstractOverwhelming evidence indicates that a high level of expression of the protein neuroglobin protects neurons in vitro, in animal models, and in humans, against cell death associated with hypoxic and amyloid insult. We have previously showed that neuroglobin protects neuronal cells from the mitochondrial pathway of apoptosis induced by the BH3 mimetic, by preventing cytochrome c‐triggered activation of caspase 9. Here, using cell and molecular biology approaches, we generated a particular neuroglobin mutant, Lys67Glu, overexpression of which confers a significant protection from the BH3 mimetic (TW‐37)‐induced apoptosis in human neuroblastoma SH‐SY5Y cells. The cumulative inhibition of caspase 9 activation is significantly enhanced in Lys67Glu neuroglobin‐expressing cells, as compared to wild‐type neuroglobin expressing cells. A multiparameter flow cytometry analysis of TW‐37‐treated cells revealed that inhibition of caspase 9 activity by Lys67Glu neuroglobin is associated with the preservation of the mitochondrial transmembrane potential (ΔψM), as well as a decreased rate of cytochrome crelease from the mitochondria. © 2012 International Society for Advancement of Cytometry
Standardizing minimal residual disease by flow cytometry for precursor B lineage acute lymphoblastic leukemia in a developing country
Authors: Patkar Nikhil , Alex Ansu Abu , B. Bargavi , Ahmed Rayaz , Abraham Aby , George Biju , Vishwabandya Auro , Srivastava Alok , Mathews Vikram 
Published: April 2012

AbstractBackground:In addition to standard risk criteria at diagnosis, minimal residual disease (MRD) following initiation of therapy is a well‐recognized risk factor to predict relapse. Literature from developing countries addressing therapeutic or laboratory practices related to MRD, is largely lacking. In a first paper from India, we describe our experience in establishing a flow cytometry‐based MRD assay for precursor B lineage ALL (BCP‐ALL) with emphasis on the assay standardization and cost.Methods:Normal templates for B cell development were established in 10 control patients using CD45, CD11a, CD38, CD20, CD10, CD19, CD58, CD34, CD123, and CD22. BCP‐ALL samples (n = 42) were characterized at diagnosis to identify a suitable marker for follow‐up during mid (D+21) and end of induction (D+33). Both, multiparametric immunophenotyping and single marker detection of LAIP were used for data analysis.Results:In 95.2% of BCP‐ALL at least two informative markers could be obtained when a minimum of four cocktail combinations were used. The combination CD20, CD10, CD45, and CD19 was the most useful (71.4%) followed by combinations containing CD38 (66.7%), CD22 (57.1%), CD11a (52.4%), and CD58 (33.3%). Using our approach, 60 and 47% of patients had detectable MRD at mid and end induction time points, respectively.Conclusion:We have described a relatively cost effective MRD panel which is applicable to over 90% of patients. We hope that this data would encourage more centers in India and other resource constrained health delivery systems to develop MRD assays. © 2012 International Clinical Cytometry Society
Optic tectum morphogenesis: A step‐by‐step model based on the temporal–spatial organization of the cell proliferation. Significance of deterministic and stochastic components subsumed in the spatial organization
Authors: Rapacioli Melina , Duarte Santiago , Rodríguez Celín Alejandra , Fiore Luciano , Teruel Luisa , Scicolone Gabriel , Sánchez Viviana , Flores Vladimir 
Published: April 2012

AbstractBackground: Cell proliferation plays an important morphogenetic role. This work analyzes the temporal–spatial organization of cell proliferation as an attempt to understand its contribution to the chick optic tectum (OT) morphogenesis. Results: A morphogenetic model based on space‐dependent differences in cell proliferation is presented. Step1: a medial zone of high mitotic density (mZHMD) appears at the caudal zone. Step2: the mZHMD expands cephalically forming the dorsal curvature and then duplicates into two bilateral ZHMDs (bZHMD). Step3: the bZHMDs move toward the central region of each hemitectum. Step4: the planar expansion of both bZHMD and a relative decrement in the dorsal midline growth produces a dorsal medial groove separating the tectal hemispheres. Step5: a relative caudal displacement of the bZHMDs produces the OT caudal curvature. Numerical sequences derived from records of mitotic cells spatial coordinates, analyzed as stochastic point processes, show that they correspond to 1/f(β) processes. The spatial organization subsumes deterministic and stochastic components. Conclusions: The deterministic component describes the presence of a long‐range influence that installs an asymmetric distribution of cell proliferation, i.e., an asymmetrically located ZHMD that print space‐dependent differences onto the tectal corticogenesis. The stochastic component reveals short‐range anti‐correlations reflecting spatial clusterization and synchronization between neighboring cells. Developmental Dynamics 241:1043–1061, 2012. © 2012 Wiley Periodicals, Inc.
Biphasic wnt8a expression is achieved through interactions of multiple regulatory inputs
Authors: Narayanan Anand , Lekven Arne C. 
Published: April 2012

AbstractBackground: Vertebrate axis development depends upon wnt8a transcription in a dynamic pool of mesoderm progenitors at the posterior pole of the gastrulating embryo. The transcriptional mechanisms controlling wnt8a expression are not understood, but previous studies identified two phases of wnt8a expression in zebrafish: Nodal‐dependent activation during early gastrulation (phase I) and No tail (Ntl)‐dependent regulation from mid gastrula stages (phase II). Results: We identified two upstream cis‐regulatory regions, proximal and distal, each of which possesses a promoter. The proximal regulatory region contains a margin‐specific enhancer that is required for both the Nodal and Ntl responses. Phase I expression requires Nodal activation of the margin enhancer in combination with the transcription factor Zbtb4 and the distal regulatory region. Phase II expression requires Ntl regulation of the margin enhancer in the context of the proximal regulatory region. An additional mechanism is required to ensure the transition from phase I to phase II regulation. Analysis of stickleback wnt8a suggests this mechanism of regulation may be conserved. Conclusions: The seemingly simple wnt8a expression pattern reflects complex interactions of multiple regulatory inputs. Developmental Dynamics 241:1062–1075, 2012. © 2012 Wiley Periodicals, Inc.
Retracted: Human factors of knowledge sharing intention among taiwanese enterprises: a preliminary study
Authors: Tseng Chun‐Pin , Chang Min‐Li , Chen Cheng‐Wu 
Published: April 2012

AbstractKnowledge management (KM) is very important in the business world of today. The Taiwanese government has recognized the importance of KM in helping small and medium enterprises (SMEs) to innovate through both their internal core competencies and external resources. They have the KM promotion project to combine resources from the information service industry and the academic sector in order to assist SMEs, and have been attempting to introduce the process of KM since 1993. The motivation, opportunity, and ability (MOA) framework has been applied to study how this MOA availability drives knowledge sharing in large enterprises. This study investigates the factors or barriers that influence the intention of knowledge sharing in Taiwanese SMEs through the MOA framework. It should help business managers identify the motivational elements that can encourage investment in it, and then propose pragmatic suggestions for introducing KM initiatives in order to reinvigorate the numbers of KM‐implementing SMEs in Taiwan. © 2012 Wiley Periodicals, Inc.
V-ATPase subunit ATP6AP1 (Ac45) regulates osteoclast differentiation, extracellular acidification, lysosomal trafficking, and protease exocytosis in osteoclast-mediated bone resorption
Authors: Yang De-Qin , Feng Shengmei , Chen Wei , Zhao Haibo , Paulson Christie , Li Yi-Ping 
Published: April 2012

Abstract
Lysosomal trafficking and protease exocytosis in osteoclasts are essential for ruffled border formation and bone resorption. Yet the mechanism underlying lysosomal trafficking and the related process of exocytosis remains largely unknown. We found ATP6ap1 (Ac45), an accessory subunit of vacuolar-type H+-ATPases (V-ATPases), to be highly induced by receptor activator for nuclear factor kappa B ligand (RANKL) in osteoclast differentiation. Ac45 knockdown osteoclasts formed normal actin rings, but had severely impaired extracellular acidification and bone resorption. Ac45 knockdown significantly reduced osteoclast formation. The decrease in the number of osteoclasts does not result from abnormal apoptosis; rather, it results from decreased osteoclast precursor cell proliferation and fusion, which may be partially due to the downregulation of extracellular signal-regulated kinase (ERK) phosphorylation and FBJ osteosarcoma oncogene (c-fos), nuclear factor of activated T-cells, cytoplasmic 1 (NFATc1), and “transmembrane 7 superfamily member 4” (Tm7sf4) expression. Notably, Ac45 knockdown osteoclasts exhibited impaired lysosomal trafficking and exocytosis, as indicated by the absence of lysosomal trafficking to the ruffled border and a lack of cathepsin K exocytosis into the resorption lacuna. Our data revealed that the impaired exocytosis is specifically due to Ac45 deficiency, and not the general consequence of a defective V-ATPase. Together, our results demonstrate the essential role of Ac45 in osteoclast-mediated extracellular acidification and protease exocytosis, as well as the ability of Ac45 to guide lysosomal intracellular trafficking to the ruffled border, potentially through its interaction with the small guanosine-5′-triphosphatase (GTPase) Rab7. Our work indicates that Ac45 may be a novel therapeutic target for osteolytic disease. © 2012 American Society for Bone and Mineral Research.
Determination of the rate constants of the reactions of NO3 radical with CF3I and I(2P3/2)
Authors: Nakano Yukio , Sadamori Kengo , Ishiwata Takashi 
Published: April 2012

AbstractThe rate constant of the reaction of NO3 radical with CF3I was determined by a relative rate method using a Fourier transform infrared (FTIR) spectrometer smog chamber with a long path length gas cell. As a result, the upper limit of the rate constant was determined to be <2.2 × 10−18 cm3 molecule−1 s−1 in 100 Torr of total pressure at 298 K, which suggests that CF3I emitted into the atmosphere accumulates during night. The rate constant of the reaction of NO3 with I(2P3/2) was also measured using time‐resolved cavity ring‐down spectroscopy. The rate constant showed no pressure dependence in the range of 100–500 Torr of total pressure at 298 K and was determined to be (3.9 ± 1.0) × 10−11 cm3 molecule−1 s−1. The present result is different from the previous ones and is about half of that reported most recently. From this rate constant, it is likely that the reaction of NO3 radical with I(2P3/2) does not have a much influence on the loss of I(2P3/2) and the formation cycle of IO radical in the atmosphere. © 2012 Wiley Periodicals, Inc. Int J Chem Kinet 44: 649–660, 2012
Molecular characterization of a cancer‐related single nucleotide polymorphism in the pro‐inflammatory interleukin‐1B gene
Authors: Landvik N.E. , Tekpli X. , Anmarkrud K.H. , Haugen A. , Zienolddiny S. 
Published: April 2012

AbstractInterleukin‐1β is a key pro‐inflammatory cytokine that has been associated with chronic inflammation and inflammation‐related cancer initiation and progression. There are inter‐individual differences in IL1B expression which may be due to single nucleotide polymorphisms (SNPs) in the regulatory regions of the gene. We have previously shown that a SNP located in the promoter of the IL1B gene (the IL1B T‐31C SNP) was associated with lung cancer risk. Interestingly, the presence of the C allele was also associated with reduced IL1B expression in normal lung tissue of lung cancer patients. In the present study, we found that differential binding patterns of nuclear proteins to oligonucleotide probes containing the IL1B ‐31C allele compared to those with the T allele were due to specific binding of the transcription factor Yin Yang 1 (YY1). We further found evidence that specific recruitment of YY1 to the ‐31C region of the IL1B promoter regulated IL1B gene expression using siRNA directed towards YY1. The results indicate that the presence of a C allele at the ‐31 position may lead to decreased expression of the IL1B gene due to a specific binding of YY1 in lung epithelial cells. Our study provides functional significance of allelic variation at a single locus in the IL1B promoter and contributes to understanding the regulation of IL1B in inflammation‐related carcinogenesis. © 2012 Wiley Periodicals, Inc.
An oocyte‐preferential histone mRNA stem‐loop‐binding protein like is expressed in several mammalian species
Authors: Thelie Aurore , Pascal Geraldine , Angulo Leslie , Perreau Christine , Papillier Pascal , Dalbies‐Tran Rozenn 
Published: April 2012

AbstractDuring early embryo development, chromatin packaging is sustained by histones of maternal origin. Most histone messenger RNAs are not polyadenylated, but rather end in an evolutionarily conserved stem‐loop that controls RNA processing, nucleocytoplasmic transport, stability, and translation via interactions with a specific protein named stem‐loop‐binding protein (SLBP). In mouse oocytes, mSLBP is synthesized abundantly during maturation and activates histone translation. In Xenopus, xSLBP is present in stage‐VI oocytes, but histone mRNA is protected from premature translation by the oocyte‐specific Xenopus SLBP2 (xSLBP2) protein; during maturation xSLBP2 replacement by xSLBP results in histone synthesis. Here, we report the first experimental evidence and characterization of a mammalian SLBP2 ortholog. Bovine bSLBP and bSLBP2 display distinct expression patterns throughout oocyte maturation and pre‐implantation embryo development. From the immature oocyte to the morula, bSLBP2 is concentrated in the nucleus, while it is homogeneously distributed throughout the cytoplasm in mature oocytes. A putative SLBP2 gene is conserved in the genome of several mammalian species, and the corresponding transcripts were detected in rat, dog, horse, and pig oocytes. By contrast, a pseudogene is found in mouse, human, and rabbit. Altogether, our data suggest that the availability of histones in oocytes is regulated by an alternative mechanism in bovine and other species as compared to mouse and frog. Mol. Reprod. Dev. 79:380–391, 2012. © 2012 Wiley Periodicals, Inc.
Dyspnea in a patient with raynaud's phenomenon: The uncovering of interstitial lung disease
Authors: Coates Anne , Meehan Richard , Milla Carlos 
Published: April 2012

AbstractInterstitial lung disease (ILD) can develop in patients with connective tissue disease (CTD) in the context of progressive multiorgan involvement, but ILD can also be the predominant manifestation of active CTD. A high index of suspicion for CTD in patients presenting with pulmonary disease might facilitate timely, accurate diagnosis and management. Pediatr Pulmonol. 2012. 47:926–927. © 2012 Wiley Periodicals, Inc.
Differences in the Kinematics of Restrained and Unrestrained Conditions of Opening for Two Sizes of Glass Jar
Authors: Bush Tamara Reid , Bix Laura , Bello Nora , Fair Joseph 
Published: April 2012

BackgroundOpening glass jars has been noted to be particularly problematic for consumers, leading to many studies related to the opening process. However, most studies place restrictions on jar opening by forcing the jar to remain on the counter or by requiring a specific hand on the closure.ObjectiveThe objectives of this research were (i) to evaluate the effect of restrained and unrestrained conditions on kinematics of jar opening, (ii) to evaluate the effects of jar diameter on kinematics and (iii) to evaluate how user characteristics (e.g. hand size) are associated with kinematics.MethodsKinematic data were collected from 21 participants during two opening conditions using jars with two diameters. Condition 1 restrained the participant by requiring that the jar maintain contact with the counter during the opening procedure. Condition 2 was termed unrestrained and allowed the user to open the jar with no requirements on jar or hand location.ResultsWhen restrained to a counter, the closure hand produced significantly more rotation compared with the closure hand in the unrestrained condition. Differences were not found in the opening motions when comparing jar diameters. However, hand breadth and length were related to the magnitude of rotation of the closure hand.ConclusionsPlacing restrictions on the participant, the orientation or location of the jar aids data analysis but may yield results that are not indicative of the user's actual jar opening process. Impositions on user positioning should be carefully considered in studies investigating the opening process of glass jars. Copyright © 2012 John Wiley & Sons, Ltd.
Self‐consistent field treatment and analytical energy gradient of local response dispersion method
Authors: Ikabata Yasuhiro , Sato Takeshi , Nakai Hiromi 
Published: April 2012

AbstractThis study presents the self‐consistent field (SCF) treatment of the local response dispersion (LRD) method. The implementation of SCF involves the modification of the Kohn–Sham Fock matrix by adding the dispersion potential. The derivatives of atomic pseudo‐polarizabilities with respect to the density variables, which are required for evaluating the dispersion potential, are efficiently updated in the SCF procedure. Analytical energy gradient of the LRD method is also developed based on the SCF treatment. Numerical assessments of the present treatment clarified that the SCF effect brings about minor changes in both energy and electronic structure. The computational time, and number of SCF iterations, are essentially unaffected by moving from a non‐self‐consistent implementation to a self‐consistent one. For the geometry optimizations for weakly interacting systems, the inclusion of the LRD energy gradients is shown to be essential for accurately demonstrating the intermolecular geometric parameters. © 2012 Wiley Periodicals, Inc.
On exponential stability conditions of linear neutral stochastic differential systems with time‐varying delay
Authors: Cong S. 
Published: April 2012

SUMMARYWe consider a class of neutral stochastic systems with time‐varying delay and study the exponential stability in the mean square sense. We derive sufficient stability conditions via applying Lyapunov functional method along with some practical techniques. Firstly, in computing the constructed Lyapunov functional, we make use of some basic rules of Itô calculus to reduce the conservatism produced by noise because it, in principle, plays a negative role for preserving stability in the mean square sense. Also, it is an important observation that, using some slack matrices, we can create convex conditions to accommodate the computation to time‐varying delay. In the sequel, we use a perturbation approach to estimate the decay rate of state and come to the conclusion of stability. Finally, we include an example to demonstrate the effectiveness of the method. Copyright © 2012 John Wiley & Sons, Ltd.
Distribution of the number of spanning regular subgraphs in random graphs
Authors: Gao Pu 
Published: April 2012

AbstractIn this paper, we examine the moments of the number of d ‐factors in
\documentclass{article}\usepackage{mathrsfs, amsmath, amssymb}\pagestyle{empty}\begin{document}\begin{align*}\mathcal{ G}(n,p)\end{align*} \end{document}
for all p and d satisfying d3 = o(p2n). We also determine the limiting distribution of the number of d ‐factors inside this range with further restriction that
\documentclass{article}\usepackage{mathrsfs, amsmath, amssymb}\pagestyle{empty}\begin{document}\begin{align*}(1-p)\sqrt{dn}\to\infty\end{align*} \end{document}
as n →∞.© 2012 Wiley Periodicals, Inc. Random Struct. Alg., 2013
A deep subaqueous fan depositional model for the Palaeoarchaean (3.46 Ga) Marble Bar Cherts, Warrawoona Group, Western Australia
Authors: OLIVIER NICOLAS , DROMART GILLES , COLTICE NICOLAS , FLAMENT NICOLAS , REY PATRICE , SAUVESTRE RÉMI 
Published: April 2012

AbstractThe 3.46 Ga Marble Bar Chert Member of the East Pilbara Craton, Western Australia, is one of the earliest and best-preserved sedimentary successions on Earth. Here, we interpret the finely laminated thin-bedded cherts, mixed conglomeratic beds, chert breccia beds and chert folded beds of the Marble Bar Chert Member as the product of low-density turbidity currents, high-density turbidity currents, mass transport complexes and slumps, respectively. Integrated into a channel-levee depositional model, the Marble Bar Chert Member constitutes the oldest documented deep-sea fan on Earth, with thin-bedded cherts, breccia beds and slumps composing the outer levee facies tracts, and scours and conglomeratic beds representing the channel systems.