bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mobile Brennstoffzellen‐Batterie‐Hybrid‐Anwendungen
Authors: Riegel B. 
Published: May 2009

AbstractDie Brennstoffzelle in Kombination mit verschiedenen Hochleistungsbatterien ist eine gute Möglichkeit, die chemische Energie, die im Wasserstoff gebunden ist, für die Anwendung im Material‐Handling‐Bereich nutzbar zu machen. Ein derartiges System gestattet erstmalig eine echte Trennung zwischen der Bereitstellung von Energie in Form einer Brennstoffzelle zur Versorgung der Grundlastanforderung und von Leistung in Form einer Batterie zur Abdeckung hoher Lastspitzen und effizienter Nutzung von Rekuperationsenergien. Außerdem hat es den Vorteil, dass Mehrschichteinsätze gefahren werden können, ohne das Energieversorgungssystem wechseln zu müssen. Im Rahmen des von der Landesregierung Nordrhein‐Westfalen geförderten Projektes BBH‐MH „Brennstoffzellen‐Batterie‐Hybrid für Material Handling”︁ wurden drei Prototypen für den Einsatz in einem Klasse‐I‐ und zwei Klasse‐II‐Fahrzeugen durch die Firma HOPPECKE konzipiert, entworfen und erfolgreich in Betrieb genommen. Hierbei handelt es sich um ein 80‐V‐System für einen Gegengewichtsgabelstapler vom Typ Still R60‐25iL und zwei 48‐V‐Systeme für den Vertikalkommissionierer Still EK12i sowie den Schubmaststapler Still FM‐X20.
Neue keramische Werkstoffe für Hochtemperaturbrennstoffzellen
Authors: Stelter M. , Megel S. , Marschallek F. , Mosch S. 
Published: May 2009

AbstractKeramische Hochtemperaturbrennstoffzellen (SOFC) müssen bei Temperaturen von ca. 850 °C betrieben werden, um technisch attraktive elektrische Wirkungsgrade von über 50 % zu erzielen. Die erhöhten Anforderungen an die Konstruktions‐ und Aktivmaterialien im System lassen sich durch innovative Keramiklösungen erfüllen. Beispiele werden in dieser Arbeit vorgestellt.
Stand der Technik von Polymer‐Elektrolyt‐Membran‐Brennstoffzellen – ein Überblick
Authors: Heinzel A. 
Published: May 2009

AbstractWenn regenerativ erzeugter Strom, biogene Energieträger und Wasserstoff die fossilen Energieträger nach und nach ablösen, ist eine der wichtigsten Zukunftstechnologien die effiziente Wandlung von Wasserstoff in elektrische Energie und Wärme. Mittlerweile sind in verschiedensten Anwendungen Polymer‐Elektrolyt‐Membran (PEM)‐Brennstoffzellensysteme in der Erprobung. Dank gezielter und kontinuierlicher staatlicher Förderung kann es gelingen, diese neue umweltfreundliche Technologie auch in Konkurrenz zu existierenden Techniken im Markt zu etablieren. Die Arbeit gibt einen Überblick über die Entwicklung der Brennstoffzellentechnologie.
Cover Picture: Discovery of Riociguat (BAY 63‐2521): A Potent, Oral Stimulator of Soluble Guanylate Cyclase for the Treatment of Pulmonary Hypertension (ChemMedChem 5/2009)
Authors: Mittendorf Joachim , Weigand Stefan , Alonso‐Alija Cristina , Bischoff Erwin , Feurer Achim , Gerisch Michael , Kern Armin , Knorr Andreas , Lang Dieter , Muenter Klaus , Radtke Martin , Schirok Hartmut , Schlemmer Karl‐Heinz , Stahl Elke , Straub Alexander , Wunder Frank , Stasch Johannes‐Peter 
Published: May 2009

AbstractThe cover picture shows a schematic representation of soluble guanylate cyclase (sGC). This key signal‐transduction enzyme is typically found as a heterodimer, consisting of a larger α‐subunit and a smaller haeme‐binding β‐subunit. It is activated by the ubiquitous messenger nitric oxide and catalyzes the conversion of guanosine‐5′‐triphosphate (GTP) into the second messenger cyclic guanosine‐3′,5′‐monophosphate (cGMP). The depicted compound is riociguat, which was identified as a potent, oral sGC stimulator and is currently being investigated in phase III clinical trials for the treatment of pulmonary hypertension. For more details, see the Full Paper by J. Mittendorf et al. on p. 853 ff.magnified image
Inside Cover: Homology Model Adjustment and Ligand Screening with a Pseudoreceptor of the Human Histamine H4 Receptor (ChemMedChem 5/2009)
Authors: Tanrikulu Yusuf , Proschak Ewgenij , Werner Tim , Geppert Tim , Todoroff Nickolay , Klenner Alexander , Kottke Tim , Sander Kerstin , Schneider Erich , Seifert Roland , Stark Holger , Clark Timothy , Schneider Gisbert 
Published: May 2009

AbstractThe inside cover picture shows the predicted histamine H4 receptor (H4R) binding pocket showing the conformation that best fits a H4R pseudoreceptor. Potential ligand–receptor interactions are shown as spheres, where the sphere size correlates with their computed relative importance: H‐bond acceptor (red) and donor (blue), and aromatic (green) pseudoatoms. Combining MD studies with pseudoreceptor matching yields preferred ligand binding sites in GPCR models. For details, see the Full Paper by G. Schneider et al. on p. 820 ff.magnified image
Cover Picture: Local Modulation of the Redox State of p‐Nitrothiophenol Self‐Assembled Monolayers Using the Direct Mode of Scanning Electrochemical Microscopy (ChemPhysChem 7/2009)
Authors: Schwamborn Stefanie , Stoica Leonard , Neugebauer Sebastian , Reda Torsten , Schmidt Hanns‐Ludwig , Schuhmann Wolfgang 
Published: May 2009

AbstractThe cover picture illustrates the principle of molecular writing using direct‐mode scanning electrochemical microscopy (SECM). The redox state of the end groups of a p‐nitrothiophenol self‐assembled monolayer (SAM) is selectively reduced either to the hydroxylamine/nitoso redox couple or to the amino group. Besides the writing procedure, W. Schuhmann et al. demonstrate on p. 1066 that SECM can also be utilized for visualization or reading of the generated microstructure in feedback mode using hexacyanoferrate as redox mediator.magnified image
Cre/lox recombination in the lower urinary tract
Authors: Wang Yinqiu , Tripathi Piyush , Guo Qiusha , Coussens Matthew , Ma Liang , Chen Feng 
Published: May 2009

AbstractTbx18 is a T‐Box transcription factor that has specific expression and indispensible function in the lower urinary tract. Here, we report the generation and characterization of a bacterial artificial chromosome (BAC) transgene expressing Cre under the control of Tbx18 regulatory elements. When crossed to the ROSA26R‐lacZ reporter mice, the Tbx18‐Cre transgene mediates loxP recombination in the mesenchymal derivatives in the lower urinary tract, especially in the smooth muscle cells (SMCs) and the stromal cells. There is no expression of this transgene in the urothelium or in the kidney. This Tbx18‐Cre transgene recapitulates the endogenous Tbx18 expression in the urinary system and can be used for the study of the development, physiology, and diseases in the urinary tract. Its additional expression in the epicardium, limb, vibrissae, and other structures would be useful for studies in the relevant fields. genesis 47:409–413, 2009. © 2009 Wiley‐Liss, Inc.
Synthesis and Characterization of Semiconductive Dichloridobis(thianthrene)gold(1+) Tetrachloridoaurate(1–)
Authors: Tjahjanto Rachmat Triandi , Beck Johannes 
Published: May 2009

AbstractA new (thianthrene)gold(III) complex has been synthesized in liquid SO2 as the solvent from thianthrene (TA) andAuCl3. [AuCl2(TA)2][AuCl4] [triclinic, P$\bar {1}$, a = 9.9832(2) Å, b = 10.3404(2) Å, c = 15.0798(4) Å, α = 75.038(1)°, β = 81.610(1)°, γ = 68.409(1)°, V = 1396.15(5) Å3, Z = 2] has a salt‐like structure consisting of [AuCl2(TA)2]+ and [AuCl4]– ions, both with square‐planar coordinated gold atoms of oxidation state +3. In the cation, two bent TA molecules are coordinated to Au each through one sulfur atom. The title compound is thermally stable up to 425 K and is semiconducting with a conductivity reaching 25 mSm–1 at 380 K and a low activation energy of 0.43 eV. A model for the charge transport along the stacked cationic complexes is discussed. When dissolved in chloroform [AuCl2(TA)2][AuCl4] is converted into the already known uncharged, mononuclear complex [AuCl3(TA)], which shows that a polymerization isomerism exists between the two forms.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Five‐Membered Cyclic Nitronates in C–C Coupling with 1‐(tert‐Butyldimethylsilyloxy)‐1‐methoxyethylene
Authors: Smirnov Vladimir O. , Sidorenkov Alexander S. , Khomutova Yulia A. , Ioffe Sema L. , Tartakovsky Vladimir A. 
Published: May 2009

AbstractFive‐membered cyclic nitronates 3 undergo TBSOTf‐catalysed C–C coupling reactions with silyl ketene acetal 4 to give good to excellent yields of 3,3‐disubstituted N‐(OTBS)‐isoxazolidines 5, which could not be obtained by previously known procedures. The problems of diastereoselectivity of the C–C coupling reactions are discussed. The transformations of the nitroso acetals 5 upon action of AcFOH/methanol or some reducing agents (H2/NiRa, LAH, Al/Hg) have been examined.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Evaluation of 6‐APA as a New Organocatalyst for a Direct Cross‐Aldol Reaction
Authors: Emer Enrico , Galletti Paola , Giacomini Daria 
Published: May 2009

Abstract6‐Aminopenicillanic acid (6‐APA) and two of its derivatives, 6‐APA benzyl ester (6‐APA‐OBn) and Penicillin G (PenG), have been evaluated as catalysts for use in direct cross‐aldol reactions in different solvents and mixtures. The effects of catalyst loading, reaction time, pH and temperature on the yield and stereoselectivity have been studied. 6‐APA proved to be an effective catalyst in terms of yield for the reaction between cyclohexanone and p‐nitrobenzaldehyde, especially in neat conditions. The stereoselectivity of the reaction was conditioned by the presence of two competing mechanisms: one an imine–enamine pathway and the other a Brønsted acid catalysis. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Phosphane‐Free Palladium‐Catalyzed Carbonylative Suzuki Coupling Reaction of Aryl and Heteroaryl Iodides
Authors: Tambade Pawan J. , Patil Yogesh P. , Panda Anil G. , Bhanage Bhalchandra M. 
Published: May 2009

AbstractThe carbonylative cross‐coupling reaction of an arylboronic acid with an aryl iodide and a heteroaryl iodide to give unsymmetrical biaryl ketones was carried out by using Pd(tmhd)2/Pd(OAc)2 as a phosphane‐free catalyst. Various aryl and heteroaryl iodides with different arylboronic acid derivatives provided good to excellent yields of the desired products under optimized reaction conditions.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Detection of C Reactive Protein (CRP) in Serum by an Electrochemical Aptamer‐Based Sandwich Assay
Authors: Centi Sonia , Bonel Sanmartin Laura , Tombelli Sara , Palchetti Ilaria , Mascini Marco 
Published: May 2009

AbstractA disposable electrochemical assay involving magnetic particles and carbon‐based screen‐printed electrodes (SPCEs) was developed for the detection of C Reactive Protein (CRP). CRP is a plasma protein and is among the most expressed proteins in acute phase inflammation cases, being a known biomarker for inflammatory states. The assay was based on a sandwich format in which a RNA aptamer was coupled to a monoclonal antibody and alkaline phosphatase (AP) was used as enzymatic label. After the sandwich assay, the modified magnetic beads were captured by a magnet on the surface of a graphite working electrode and the electrochemical detection was thus achieved through the addition of the AP substrate (α‐naphthyl‐phosphate) and α‐naphthol produced during the enzymatic reaction was detected using differential pulse voltammetry (DPV). The parameters influencing the different steps of the assay were optimized in order to reach the best sensitivity and specificity. With the optimized conditions, the assay was applied to the analysis of CRP free serum and serum samples.
Aptamer‐Based Electrochemical Sensor for Label‐Free Recognition and Detection of Cancer Cells
Authors: Pan Chunfeng , Guo Manli , Nie Zhou , Xiao Xilin , Yao Shouzhuo 
Published: May 2009

AbstractAn electrochemical biosensor was developed for the label‐free and selective detection of leukemia cells based on aptamer‐modified gold electrode using electrochemical impedance spectroscopy (EIS) technique. The thiol‐terminated aptamer (sgc8c) selected for CCRF‐CEM acute leukemia cells was self‐assembled onto the gold electrode surface as recognition probe, which was characterized by cyclic voltammetry (CV) and EIS using Fe(CN)63−/4− as a redox probe. The surface density of aptamers was determined by chronocoulometric method using a redox cation of Ru(NH3)63+. Upon incubation of the aptamer‐modified electrode with CCRF‐CEM cells, the electron‐transfer resistance (Ret) of Fe(CN)63−/4− on the sensor surface increased substantially. Only a small Ret change of the sensor to the control negative cell line RAJI was observed, indicating the excellent selectivity of the sensor. The selective capture of CCRF‐CEM cells on the sensor surface was also confirmed by fluorescence microscopy. A linear relationship between Ret and the logarithmic value of CCRF‐CEM cells concentration was found in the range of 1×104 to 1×107 cells/mL, with a detection limit of 6×103 cells/mL. This work provided a simple, convenient, low‐cost and label‐free method for early leukemia diagnosis.
Upregulation of plakophilin‐2 and its acquisition to adherens junctions identifies a novel molecular ensemble of cell–cell‐attachment characteristic for transformed mesenchymal cells
Authors: Rickelt Steffen , Winter‐Simanowski Stefanie , Noffz Edeltraut , Kuhn Caecilia , Franke Werner Wilhelm 
Published: May 2009

AbstractIn contrast to the desmosome‐containing epithelial and carcinoma cells, normal and malignantly transformed cells derived from mesenchymal tissues and tumors are connected only by adherens junctions (AJs) containing N‐cadherins and/or cadherin‐11, anchored in a cytoplasmic plaque assembled by α‐ and β‐catenin, plakoglobin, proteins p120 and p0071. Here, we report that the AJs of many malignantly transformed cell lines are characterized by the additional presence of plakophilin‐2 (Pkp2), a protein hitherto known only as a major component of desmosomal plaques, i.e., AJs of epithelia and carcinomatous cells. This massive acquisition of Pkp2 and its integration into AJ plaques of a large number of transformed cell lines is demonstrated with biochemical and immunolocalization techniques. Upregulation of Pkp2 and its integration into AJs has also been noted in some soft tissue tumors insitu and some highly proliferative colonies of cultured mesenchymal stem cells. As Pkp2 has recently been identified as a functionally important major regulatory organizer in AJs and related junctions in epithelial cells and cardiomyocytes, we hypothesize that the integration of Pkp2 into AJs of “soft tissue tumor” cells also can serve functions in the upregulation of proliferation, the promotion of malignant growth in general as well as the close‐packing of diverse kinds of cells and the metastatic behavior of such tumors. We propose to examine its presence in transformed mesenchymal cells and related tumors and to use it as an additional diagnostic criterion. © 2009 UICC
Cooperative interaction between protein inhibitor of activated signal transducer and activator of transcription‐3 with epidermal growth factor receptor blockade in lung cancer
Authors: Kluge Amy , Dabir Snehal , Kern Jeffrey , Nethery David , Halmos Balazs , Ma Patrick , Dowlati Afshin 
Published: May 2009

AbstractEpidermal Growth Factor Receptor (EGFR) targeting in nonsmall cell lung cancer (NSCLC) is an established treatment modality; however, it only benefits a minority of patients. STAT3 (signal transducer and activator of transcription‐3) plays an important role in the oncogenic signal transduction pathway of NSCLC. Inhibition of STAT3 results in NSCLC growth inhibition and apoptosis. We have previously shown that combined inhibition of EGFR and STAT3 by small molecules resulted in improved therapeutic efficacy as compared with blocking EGFR alone. However, the STAT3 protein has a number of endogenous negative regulators including PIAS3 (Protein Inhibitor of Activated STAT3). In this study, we investigated for the first time the role of PIAS3 in modulating oncogenic EGFR‐STAT3 signaling pathway in lung cancer and the anti‐proliferative effect of using PIAS3 in conjunction with EGFR blockade in NSCLC. We demonstrate that PIAS3 is expressed in variable degrees in all NSCLC cells. EGF and IL‐6 stimulation resulted in the association of PIAS3 with STAT3. The PIAS3/STAT3 complex then bound the STAT3 DNA binding sequence resulting in STAT3 regulated gene expression. Over‐expression of PIAS3, using a PIAS3 expression construct, decreases STAT3 transcriptional activity. Furthermore, over‐expression of PIAS3 consistently decreased proliferation. EGFR blockade and PIAS3 over‐expression in combination had significantly greater anti‐proliferative effects as compared with either EGFR blockade or PIAS3 over‐expression alone. In conclusion, PIAS3 is expressed in NSCLC cell lines and its over‐expression decreased STAT3 transcriptional activity, decreased proliferation of NSCLC cells and when used in conjunction with EGFR inhibitors, increased the anti‐proliferative effects. © 2009 UICC
Dietary glucosinolate intake and risk of prostate cancer in the EPIC‐Heidelberg cohort study
Authors: Steinbrecher Astrid , Nimptsch Katharina , Hüsing Anika , Rohrmann Sabine , Linseisen Jakob 
Published: May 2009

AbstractGlucosinolates (GLS) are secondary plant metabolites occurring in cruciferous vegetables. Their biologically active break‐down products show cancer preventive properties in animal and cell studies. So far, epidemiologic studies, using consumption of cruciferous vegetables as proxy for GLS intake, yielded inconsistent results. Here, we evaluated the association between dietary intake of GLS in comparison with consumption data of GLS‐containing foods and the risk of prostate cancer. The study population comprised 11,405 male participants of the prospective EPIC‐Heidelberg cohort study. During a mean follow‐up time of 9.4 years, 328 incident cases of prostate cancer occurred. At recruitment, habitual food consumption was assessed by a validated food frequency questionnaire, and intake of individual GLS was estimated by means of a newly compiled database on food content of GLS. Adjusted hazard ratios (HR) for prostate cancer were calculated using the Cox proportional hazard model. Median daily intake of total GLS was 7.9 mg/day (interquartile range 5.1–11.9 mg/day). The risk of prostate cancer decreased significantly over quartiles of total GLS intake (multivariate HR [4th vs. 1st quartile] 0.68, 95% CI 0.48–0.97, ptrend 0.03). Associations with GLS‐containing food intake were weaker. Among GLS subgroups, aliphatic GLS showed the strongest inverse association with cancer risk. Analyses stratified by tumor stage and grade gave hint to inverse associations for localized and low‐grade cancers. This study shows an inverse association between dietary intake of GLS and the risk of prostate cancer. Because this is the first prospective study using individual GLS intake data, confirmation in other studies is warranted. © 2009 UICC
Down‐regulation of HLA Class I and NKG2D ligands through a concerted action of MAPK and DNA methyltransferases in colorectal cancer cells
Authors: Sers Christine , Kuner Ruprecht , Falk Christine S. , Lund Per , Sueltmann Holger , Braun Monika , Buness Andreas , Ruschhaupt Markus , Conrad Janine , Mang‐Fatehi Shila , Stelniec Iwona , Krapfenbauer Ulf , Poustka Annemarie , Schäfer Reinhold 
Published: May 2009

AbstractMost malignant features of cancer cells are triggered by activated oncogenes and the loss of tumor suppressors due to mutation or epigenetic inactivation. It is still unclear, to what extend the escape of emerging cancer cells from recognition and elimination by the immune system is determined by similar mechanisms. We compared the transcriptomes of HCT116 colorectal cancer cells deficient in DNA methyltransferases (DNMTs) and of cells, in which the RAS pathway as the major growth‐promoting signaling system is blocked by inhibition of MAPK. We identified the MHC Class I genes HLA‐A1/A2 and the ULBP2 gene encoding 1 of the 8 known ligands of the activating NK receptor NKG2D among a cluster of immune genes up‐regulated under the conditions of both DNMT‐deficiency and MEK‐inhibition. Bisulphite sequencing analyses of HCT116 with DNMT deficiency or after MEK‐inhibition showed that de‐methylation of the ULPB2 promoter correlated with its enhanced surface expression. The HLA‐A promoters were not methylated indicating that components of the HLA assembly machinery were also suppressed in DNMT‐deficient and MEK‐inhibited cells. Increased HLA‐A2 surface expression was correlated with enhanced recognition and lysis by A2‐specific CTL. On the contrary, elevated ULBP2 expression was not reflected by enhanced recognition and lysis by NK cells. Cosuppression of HLA Class I and NKG2D ligands and genes encoding peptide transporters or proteasomal genes mediates a strong functional link between RAS activation, DNMT activity and disruption of the antigen presenting system controlling immune recognition in colorectal cancer cells. © 2009 UICC
Mesenchymal stem cell modification of endothelial matrix regulates their vascular differentiation
Authors: Lozito Thomas P. , Taboas Juan M. , Kuo Catherine K. , Tuan Rocky S. 
Published: May 2009

AbstractMesenchymal stem cells (MSCs) respond to a variety of differentiation signal provided by their local environments. A large portion of these signals originate from the extracellular matrix (ECM). At the same time, MSCs secrete various matrix‐altering agents, including proteases, that alter ECM‐encoded differentiation signals. Here we investigated the interactions between MSC and ECM produced by endothelial cells (EC‐matrix), focusing not only on the differentiation signals provided by EC‐matrix, but also on MSC‐alteration of these signals and the resultant affects on MSC differentiation. MSCs were cultured on EC‐matrix modified in one of three distinct ways. First, MSCs cultured on native EC‐matrix underwent endothelial cell (EC) differentiation early during the culture period and smooth muscle cell (SMC) differentiation at later time points. Second, MSCs cultured on crosslinked EC‐matrix, which is resistant to MSC modification, differentiated towards an EC lineage only. Third, MSCs cultured on EC‐matrix pre‐modified by MSCs underwent SMC‐differentiation only. These MSC‐induced matrix alterations were found to deplete the factors responsible for EC‐differentiation, yet activate the SMC‐differentiation factors. In conclusion, our results demonstrate that the EC‐matrix contains factors that support MSC differentiation into both ECs and SMCs, and that these factors are modified by MSC‐secreted agents. By analyzing the framework by which EC‐matrix regulates differentiation in MSCs, we have uncovered evidence of a feedback system in which MSCs are able to alter the very matrix signals acting upon them. J. Cell. Biochem. 107: 706–713, 2009. Published 2009 Wiley‐Liss, Inc.