bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamic cellular translocation of caldendrin is facilitated by the Ca2+‐myristoyl switch of recoverin
Authors: Fries Ramona , Reddy Pasham Parameshwar , Mikhaylova Marina , Haverkamp Silke , Wei Tao , Müller Michael , Kreutz Michael R. , Koch Karl‐Wilhelm 
Published: March 2010

J. Neurochem. (2010) 113, 1150–1162.AbstractCaldendrin and recoverin are Ca2+‐sensor proteins operating in neuronal systems. In a search for novel binding partners of recoverin, we employed an affinity column and identified caldendrin as a possible interaction partner. Caldendrin and recoverin co‐localized in the retina in a subset of bipolar cells and in the pineal gland as revealed by immunofluorescence studies. The binding process was controlled by Ca2+ as revealed by pull‐down assays, and surface plasmon resonance studies. Importantly, caldendrin existed as a Ca2+‐independent homodimer whereas a complex of recoverin and caldendrin formed with low to moderate affinity in the presence of Ca2+. Co‐transfection of COS‐7 cells with plasmids harboring the gene for fluorescently labeled recoverin and caldendrin was used to study the cellular distribution by time‐lapse fluorescence microscopy. Apparently, the increase of intracellular Ca2+ facilitates the translocation of caldendrin to intracellular membranes, which is under control of complex formation with recoverin.
Change in posture control after recent knee anterior cruciate ligament reconstruction?
Authors: Dauty Marc , Collon Sylvie , Dubois Charles 
Published: March 2010

SummaryThe aim of this study was to compare statical postures of a knee anterior cruciate ligament reconstruction (ACLR) population with a healthy control population. Thirty‐five patients (age 25·5 ± 5·8 years) were compared at 15 days after an anterior cruciate ligament reconstruction with 35 healthy, age and sex‐matched subjects. Bilateral and unilateral postures were studied according to various stances, knee extension and 20 degrees knee flexion with opened and closed eyes, using a stabilometric platform. A comparison with the non‐ACLR limb and the healthy limbs of the control population was carried out. The ACLR subjects present with the following: (i) a significant change in two‐legged stance, i.e. distances covered by the centre of pressure projection are significantly increased; (ii) a postural alteration during the ACLR one‐legged stance with knee extension and opened eyes in comparison with the non‐ACLR limb; (iii) an incapacity for certain ACLR subjects to perform one‐legged stance on the non‐ACLR limb when there is no visual compensation. Only 11·4% (95% CI: 0·9–21·9%) and 42·8% (95% CI: 26·3–59·3%) of ACLR subjects are capable of maintaining correctly a one‐legged stance posture with closed eyes on both sides (knee extension and flexion, respectively). The identification of the ACLR knee limb is possible from the one‐legged stance postural test in knee extension and opened eyes condition. Because of a change in two‐legged balance and of the incapacity for certain ACLR subjects to maintain one‐legged stance with closed eyes, a central origin explaining the abnormalities of postural control is suggested.
Fast track surgery: A clinical audit
Authors: CARTER Jonathan , SZABO Rebecca , SIM Wee Wee , PATHER Selvan , PHILP Shannon , NATTRESS Kath , COTTERELL Stephen , PATEL Pinki , DALRYMPLE Chris 
Published: March 2010

Background:  Fast track surgery is a concept that utilises a variety of techniques to reduce the surgical stress response, allowing a shortened length of stay, improved outcomes and decreased time to full recovery.Aims:  To evaluate a peri‐operative Fast Track Surgical Protocol (FTSP) in patients referred for abdominal surgery.Methods:  All patients undergoing a laparotomy over a 12‐month period were entered prospectively on a clinical database. Data were retrospectively analysed.Results:  Over the study period, 72 patients underwent a laparotomy. Average patient age was 54 years and average weight and BMI were 67.2 kg and 26 respectively. Sixty three (88%) patients had a vertical midline incision (VMI). There were no intraoperative blood transfusions. The median length of stay (LOS) was 3.0 days. Thirty eight patients (53%) were discharged on or before post op day 3, seven (10%) of whom were discharged on postoperative day 2. On stepwise regression analysis, the following were found to be independently associated with reduced LOS: able to tolerate early enteral nutrition, good performance status, use of COX inhibitor and transverse incision. In comparison with colleagues at the SGOG not undertaking FTS for their patients, the authors’ LOS was lower and the RANZCOG modified Quality Indicators (QI’s) did not demonstrate excess morbidity.Conclusions:  Patients undergoing fast track surgery can be discharged from hospital with a reduced LOS, without an increased readmission rate and with comparative outcomes to non‐fast tracked patients.
Evaluating therapeutic effect in symptoms of moderate‐to‐severe premenstrual syndrome with Vitex agnus castus (BNO 1095) in Chinese women
Authors: MA Linlin , LIN Shouqing , CHEN Rong , ZHANG Ying , CHEN Fengling , WANG Xiuli 
Published: March 2010

Objectives:  To assess therapeutic effect of an extract of Vitex agnus castus (VAC, BNO 1095) in premenstrual syndrome (PMS) in Chinese women.Design:  It was a prospective, randomised, double‐blind, placebo‐controlled study carried out in China. Eligible patients were treated with VAC extract or placebo for three cycles. Symptoms were documented with PMS diary (PMSD), a daily rating scale with 17 items. Main efficacy variable was the reduction percentage of 17 symptom score documented in PMSD during the luteal phase of the third treatment cycle.Results:  A total of 67 patients were enrolled and randomly assigned to VAC group or placebo group. Of these, 64 patients completed the study (31 vs. 33). All the 17 symptoms showed a significantly greater improvement with VAC than placebo (P < 0.05) except lower abdominal cramping (P > 0.05).Conclusion:  Vitex agnus castus is more effective than placebo in the treatment of moderate‐to‐severe PMS in Chinese women, especially in symptoms of negative effect and insomnia.
Cue Integration With Categories: Weighting Acoustic Cues in Speech Using Unsupervised Learning and Distributional Statistics
Authors: Toscano Joseph C. , McMurray Bob 
Published: March 2010

AbstractDuring speech perception, listeners make judgments about the phonological category of sounds by taking advantage of multiple acoustic cues for each phonological contrast. Perceptual experiments have shown that listeners weight these cues differently. How do listeners weight and combine acoustic cues to arrive at an overall estimate of the category for a speech sound? Here, we present several simulations using a mixture of Gaussians models that learn cue weights and combine cues on the basis of their distributional statistics. We show that a cue‐weighting metric in which cues receive weight as a function of their reliability at distinguishing phonological categories provides a good fit to the perceptual data obtained from human listeners, but only when these weights emerge through the dynamics of learning. These results suggest that cue weights can be readily extracted from the speech signal through unsupervised learning processes.
Space and Time in the Child’s Mind: Evidence for a Cross‐Dimensional Asymmetry
Authors: Casasanto Daniel , Fotakopoulou Olga , Boroditsky Lera 
Published: March 2010

AbstractWhat is the relationship between space and time in the human mind? Studies in adults show an asymmetric relationship between mental representations of these basic dimensions of experience: Representations of time depend on space more than representations of space depend on time. Here we investigated the relationship between space and time in the developing mind. Native Greek‐speaking children watched movies of two animals traveling along parallel paths for different distances or durations and judged the spatial and temporal aspects of these events (e.g., Which animal went for a longer distance, or a longer time?). Results showed a reliable cross‐dimensional asymmetry. For the same stimuli, spatial information influenced temporal judgments more than temporal information influenced spatial judgments. This pattern was robust to variations in the age of the participants and the type of linguistic framing used to elicit responses. This finding demonstrates a continuity between space‐time representations in children and adults, and informs theories of analog magnitude representation.
Maternal Death Following Cardiopulmonary Collapse After Delivery: Amniotic Fluid Embolism or Septic Shock Due to Intrauterine Infection?
Authors: Romero Roberto , Kadar Nicholas , Vaisbuch Edi , Hassan Sonia S. 
Published: March 2010

Citation Romero R, Kadar N, Vaisbuch E, Hassan SS. Maternal death following cardiopulmonary collapse after delivery: amniotic fluid embolism or septic shock due to intrauterine infection? Am J Reprod Immunol 2010; 64: 113–125Problem  The amniotic fluid embolism (AFE) syndrome is a catastrophic complication of pregnancy frequently associated with maternal death. The causes and mechanisms of disease responsible for this syndrome remain elusive.Method of study  We report two cases of maternal deaths attributed to AFE: (1) one woman presented with spontaneous labor at term, developed intrapartum fever, and after delivery had sudden cardiovascular collapse and disseminated intravascular coagulation (DIC), leading to death; (2) another woman presented with preterm labor and foul‐smelling amniotic fluid, underwent a Cesarean section for fetal distress, and also had postpartum cardiovascular collapse and DIC, leading to death.Results  Of major importance is that in both cases, the maternal plasma concentration of tumor necrosis factor‐α at the time of admission to the hospital and when patients had no clinical evidence of infection was in the lethal range (a lethal range is considered to be above 0.1 ng/mL).Conclusion  We propose that subclinical intraamniotic infection may be a cause of postpartum cardiovascular collapse and DIC and resemble AFE. Thus, some patients with the clinical diagnosis of AFE may have infection/systemic inflammation as a mechanism of disease. These observations have implications for the understanding of the mechanisms of disease of patients who develop cardiovascular collapse and DIC, frequently attributed to AFE. It may be possible to identify a subset of patients who have biochemical and immunological evidence of systemic inflammation at the time of admission, and before a catastrophic event occurs.
Isolation, polymorphism and expression study of two distinct major histocompatibility complex class II B genes from half‐smooth tongue sole (Cynoglossus semilaevis)
Authors: Li C. , Yu Y. , Sun Y. , Li S. , Zhong Q. , Wang X. , Wang Z. , Qi J. , Zhang Q. 
Published: March 2010

SummaryMajor histocompatibility complex (MHC) class II antigens are important in vertebrate immune system, which present peptides to CD4+ T cells. In the present study, cDNAs encoding MHC class II B gene were isolated from the cDNA library of half‐smooth tongue sole (Cynoglossus semilaevis), and the full length cDNA sequences were got by rapid amplification of cDNA ends polymerase chain reaction. The polymorphism of its open reading frame, 3′ untranslated region and intron 1 was studied. Nineteen class II B alleles were identified from nine individuals and clustered into two groups, designated as Cyse‐DAB and Cyse‐DBB. The deduced amino acid sequences among Cyse‐DAB and Cyse‐DBB alleles shared identities from 94.0% to 99.6% and 92.4% to 99.6%, respectively, while the identities between Cyse‐DAB and Cyse‐DBB genes varied from 85.1% to 92.0%. Three Cyse‐DAB alleles and one Cyse‐DBB allele were observed in each of two individuals, and three Cyse‐DBB alleles and one Cyse‐DAB allele in another individual, which indicated at least two loci existed in each gene. Two different 3′ UTR sequences were identified and one belonged to Cyse‐DAB, the other belonged to Cyse‐DBB. Both five Cyse‐DAB and five Cyse‐DBB intron 1 sequences were identified from genomic sequences, among which two sequences of each gene were identified in a single individual, which suggested the existence of at least two Cyse‐DAB and two Cyse‐DBB loci. Both the two genes’‐specific tissue and developmental stage expression were studied by reverse‐transcription polymerase chain reaction, which showed that the two genes had similar expression patterns in tissue study with high expression in spleen and kidney, low expression in liver, gill and ovary, moderate expression in brain, heart, intestine and testis. While in developmental analysis, Cyse‐DBB had higher expression than Cyse‐DAB in early developmental stages, which indicated that the two genes might have different functions in those stages. Therefore, in half‐smooth tongue sole, two different MHC class II B genes exist and could differentiate from each other whether by sequence analysis or by developmental stage expression study.
Influenza and respiratory disease surveillance: the US military’s global laboratory‐based network
Authors: Jeremy Sueker J. , Blazes David L. , Johns Matthew C. , Blair Patrick J. , Sjoberg Paul A. , Tjaden Jeffrey A. , Montgomery Joel M. , Pavlin Julie A. , Schnabel David C. , Eick Angelia A. , Tobias Steven , Quintana Miguel , Vest Kelly G. , Burke Ronald L. , Lindler Luther E. , Mansfield Jay L. , Erickson Ralph Loren , Russell Kevin L. , Sanchez Jose L. ,   for the DoD Influenza Working Group
Published: March 2010

Please cite this paper as: Jeremy Sueker et al. (2010) Influenza and respiratory disease surveillance: the US military’s global laboratory‐based network. Influenza and Other Respiratory Viruses 4(3), 155–161.The US Department of Defense influenza surveillance system now spans nearly 500 sites in 75 countries, including active duty US military and dependent populations as well as host‐country civilian and military personnel. This system represents a major part of the US Government’s contributions to the World Health Organization’s Global Influenza Surveillance Network and addresses Presidential Directive NSTC‐7 to expand global surveillance, training, research and response to emerging infectious disease threats. Since 2006, the system has expanded significantly in response to rising pandemic influenza concerns. The expanded system has played a critical role in the detection and monitoring of ongoing H5N1 outbreaks worldwide as well as in the initial detection of, and response to, the current (H1N1) 2009 influenza pandemic. This article describes the system, details its contributions and the critical gaps that it is filling, and discusses future plans.
What type of hedgerows do Brown hairstreak (Thecla betulaeL.) butterflies prefer? Implications for European agricultural landscape conservation
Authors: MERCKX THOMAS , BERWAERTS KOEN 
Published: March 2010

Abstract. 1. Agricultural intensification is the main driver of global biodiversity loss. Agri‐environment schemes (AES) are policy tools to counter this, but they need to be made more effective.2. Here, we focus on the resource quality of hedgerows and woodland edges, which are widespread elements of most agricultural landscapes in Europe. We analyse a set of structural factors and assess their relative importance for the Brown hairstreak butterfly. This species suffered severe declines because of agricultural intensification that may be indicative of changes for other widespread insect species that use hedgerows as resources.3. Egg‐deposition preferences were assessed by comparing egg densities among hostplant sections in two study landscapes. All sections were systematically searched during four consecutive years, resulting in 745 observed eggs.4. We demonstrate that the ground plan outline and aspect of landscape elements, the relative position within landscape elements, and the amount of young hostplant growth are particularly relevant in explaining observed egg densities, and we link their importance with the butterfly’s behavioural biology.5. Our study provides evidence that management focused on providing ample young growth, and transforming the landscape element ground plan outline from linear to a scalloped pattern, would benefit ectothermic species by providing more sheltered micro‐climates when they use these structural resources for breeding, feeding and moving through typically exposed agricultural landscapes. We believe that integrating such management options within general AES would translate into effective, large‐scale conservation measures for Brown hairstreaks and other species alike.
Patch connectivity and sand dynamics affect dispersal‐related morphology of the blue‐winged grasshopperOedipoda caerulescensin coastal grey dunes
Authors: HEIDINGER INA MONIKA MARGRET , HEIN SILKE , BONTE DRIES 
Published: March 2010

Abstract. 1. Anthropogenic land use leads to increasing habitat fragmentation and habitat loss for many species. Exchange of individuals between and recolonisation of habitat patches is essential for the stability and long‐term survival of populations in fragmented landscapes. Inter‐patch dispersal depends on the dispersal ability of species and on landscape structure.2. In this study, we analysed the influence of patch size, patch connectivity and sand dynamics on thorax‐ and wing length as proxies for dispersal ability of the blue‐winged grasshopperOedipoda caerulescensin coastal grey dunes.3. Our study revealed that sand dynamics and patch configuration had clear though sex‐specific effects on dispersal‐related morphology. Males were smaller and had shorter wings if patches became larger and less connected. In addition, both sexes were larger in habitat patches with high sand dynamics compared to those in patches with lower dynamics. Investments in wing length were only larger in connected populations when sand dynamics were low, indicating that both landscape and patch‐related environmental factors are of importance.4. The observed effect of landscape characteristics on morphology agrees with predictions from theoretical models on the evolution of dispersal in metapopulations. However, as effects appear to be less pronounced in females, it is possible that selection pressures act differently on both sexes. An alternative explanation, however, would be that the observed patterns could be due to sex‐specific phenotypic plasticity. The presented results add to the evidence that dispersal‐related morphology varies according to expectations on dispersal evolution in recently structured populations at small spatial scales.
Problem Department
Authors: Willerding Margaret F. 
Published: March 2010

This department aims to provide problems of varying degrees of difficulty which will interest anyone engaged in the study of mathematics.All readers are invited to propose problems and to solve problems here proposed. Drawings to illustrate the problems should be well done in India ink. Problems and solutions will be credited to their authors. Each solution or proposed problem sent the Editor should have the author's name introducing the problem or solution as on the following pages.The Editor of the Department desires to serve her readers by making it interesting and helpful to them. Address suggestions and problems to Margaret F. Willerding, San Diego State College, San Diego, Calif., 92115.
Problem Department
Authors: Willerding Margaret F. 
Published: March 2010

This department aims to provide problems of varying degrees of difficulty which will interest anyone engaged in the study of mathematics.All readers are invited to propose problems and to solve problems here proposed. Drawings to illustrate the problems should be well done in India ink. Problems and solutions will be credited to their authors. Each solution or proposed problem sent the Editor should have the author's name introducing the problem or solution as on the following pages.The Editor of the Department desires to serve her readers by making it interesting and helpful to them. Address suggestions and problems to Margaret F. Willerding, Mathematics Dept., California State University, San Diego, Calif. 92115.
Diagnostic investigation and surgical management of an oesophageal mural inclusion cyst in a pony yearling
Authors: Woodford N. S. , Randle T. J. L. , McCluskie L. K. 
Published: March 2010

SummaryThe purpose of this paper is to provide a concise illustrative description of the diagnostic techniques and surgical treatment of an oesophageal mural inclusion cyst in a pony.
Laryngeal paralysis secondary to lead toxicosis
Authors: Allen K. J. 
Published: March 2010

SummaryA 13‐year‐old Irish Draught cross gelding was referred for abnormal respiratory noise at low level exercise. Bilateral laryngeal paralysis was observed during the resting endoscopic examination. Blood samples revealed lead toxicosis and subsequent soil analysis confirmed high lead levels. Treatment with the lead chelating agent calcium disodium ethylenediamine tetraacetic acid was successful in rapidly reducing blood lead levels; however, complications were observed during this treatment. Despite bilateral laryngeal paralysis, the horse maintained a good quality of life and following placement of a tracheostomy tube was able to continue ridden exercise. Two years after treatment right sided laryngeal function appeared normal; however, agrade 5(of 5) left laryngeal hemiplegia was still present. Prolonged time periods are required for improvements in peripheral neuropathy subsequent to lead toxicosis to occur.
Phosphorylation of MgrA and Its Effect on Expression of the NorA and NorB Efflux Pumps of
Staphylococcus aureus
Authors: Truong-Bolduc Que Chi , Hooper David C. 
Published: March 2010

ABSTRACT

MgrA is a global regulator in
Staphylococcus aureus
that controls the expression of diverse genes encoding virulence factors and multidrug resistance (MDR) efflux transporters. We identified
pknB
, which encodes the (Ser/Thr) kinase PknB, in the
S. aureus
genome. PknB was able to autophosphorylate as well as phosphorylate purified MgrA. We demonstrated that
rsbU
, which encodes a Ser/Thr phosphatase and is involved in the activation of the SigB regulon, was able to dephosphorylate MgrA-P but not PknB-P. Serines 110 and 113 of MgrA were found to be phosphorylated, and Ala substitutions at these positions resulted in reductions in the level of phosphorylation of MgrA. DNA gel shift binding assays using
norA
and
norB
promoters showed that MgrA-P was able to bind the
norB
promoter but not the
norA
promoter, a pattern which was the reverse of that for unphosphorylated MgrA. The double mutant MgrA
S110A-S113A
bound to the
norA
promoter but not the
norB
promoter. The double mutant led to a 2-fold decrease in
norA
transcripts and a 2-fold decrease in the MICs of norfloxacin and ciprofloxacin in strain RN6390. Thus, phosphorylation of MgrA results in loss of binding to the
norA
promoter, but with a gain of the ability to bind the
norB
promoter. Loss of the ability to phosphorylate MgrA by Ala substitution resulted in increased repression of
norA
expression and in reductions in susceptibilities to NorA substrates.