bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antimicrobial photodynamic therapy with methylene blue and its derivatives in animal studies: Systematic review
Authors: Cardozo Ana Paula Martin , da Silva Daniela de Fátima Teixeira , Fernandes Kristianne Porta Santos , Ferreira Rita de Cassia , Lino‐dos‐Santos‐Franco Adriana , Rodrigues Maria Fernanda Setúbal Destro , Motta Lara Jansiski , Cecatto Rebeca Boltes 
Published: June 2024

AbstractBackgroundInfections are complications in the wound healing process, and their treatment can lead to antibiotic overuse and bacterial resistance. Antimicrobial photodynamic therapy (aPDT) is used to treat infectious diseases caused by fungi, viruses, or bacteria. Methylene blue (MB) and its derivatives are commonly used dyes in antimicrobial photodynamic therapy (aPDT–MB).MethodsThis study is a PRISMA systematic review of animal models used to discuss the usefulness and therapeutic parameters of aPDT–MB or its derivatives for treating infected skin wounds.ResultsAfter an extensive literature review, 13 controlled trials totaling 261 animals were selected to evaluate skin infection by leishmaniasis and cutaneous bacterial and fungal infections. All studies found results favoring the use of aPDT–MB. Great variability in parameters was found for radiant exposure from 12 to 360 J/cm2, MB diluted in saline solution or distilled water, irradiation time from 40 to 3600 s, irradiance most commonly at a maximum of 100 mW/cm2, and wavelength used mainly in the 630–670 nm range.ConclusionMB is a safe and promising agent used as a photosensitizer in aPDT for skin‐infected lesions. There is great variability in the parameters found. Comparisons concerning concentration, irradiation time, and light intensity need to be performed.
Sunproofing from within: A deep dive into oral photoprotection strategies in dermatology
Authors: Hartmann Dan , Valenzuela Fernando 
Published: June 2024

AbstractBackgroundPhotoprotection is the first measure in the prevention and treatment of the deleterious effects that sunlight can cause on the skin. It is well known that prolonged exposure to solar radiation leads to acute and chronic complications, such as erythema, accelerated skin aging, proinflammatory and procarcinogenic effects, and eye damage, among others.MethodsA better understanding of the molecules that can protect against ultraviolet radiation and their effects will lead to improvements in skin health.ResultsMost of these effects of the sunlight are modulated by oxidative stress and proinflammatory mechanisms, therefore, the supplementation of substances that can regulate and neutralize reactive oxygen species would be beneficial for skin protection. Current evidence indicates that systemic photoprotection should be used as an adjunctive measure to topical photoprotection.ConclusionOral photoprotectors are a promising option in improving protection against damage induced by UVR, as they contain active ingredients that increase the antioxidant effects of the body, complementing other photoprotection measures. We present a review of oral photoprotectors and their effects.
On the concept of ‘actively working at making a living’
Authors: Okrent Mark 
Published: June 2024

AbstractRichard Dawkins has suggested that the explananda of Darwinian explanations are the existence and character of entities that actively work at making a living. This paper discusses three issues related to this suggestion. First, it offers an account of what it is to actively work at making a living in terms of counterfactuals. Second, the paper articulates why it is that the existence and character of entities that actively work at making a living might require a different kind of explanation than those on offer in the sciences of physics and chemistry. Finally, it discusses why it is that it seems appropriate to use teleological locutions in the description of the behavior, structure, and metabolism of entities that actively work at making a living.
The flip side of the China syndrome: Local labor market effects in China
Authors: Ouyang Difei , Yuan Weidi 
Published: June 2024

AbstractUS–China trade is widely thought to have contributed significantly to the decline in US manufacturing employment, sometimes called the China syndrome. Flipping the point of view, we examine its impact on Chinese local labor markets between 2000 and 2010. In prefectures most exposed to export growth, there is a pronounced increase in manufacturing employment, primarily among young, low‐educated, rural people and more for blue‐collar workers. Beyond manufacturing, there is a decline in agricultural, yet an expansion in service employment, exhibiting substantial heterogeneity across demographics. The export‐driven structural transformation is profound and long‐lasting out to 2020. Among nonworking people, export growth primarily increases school attendance and the share of people relying on family support, suggesting an income effect.
“To Indulge the Tears of Women and Children”: Masculinity, Violence, and Mercy in the Conquest of the Caucasus
Authors: Campbell Ian W. 
Published: June 2024

AbstractThis article uses campaign reports and memoir literature to explore tsarist officers’ views of masculinity—both their own and that of their opponents—during the conquest of the Caucasus, focusing particularly on the Nicolaevan era. It frames conquest as a form of cultural exchange and argues that tsarist officers’ understandings of the gender order of both their own and combatant societies were a critical component of this cultural exchange. In particular, stereotypically feminine traits like mercy and gentleness were important to the cultural script of conquest in the Caucasus. To offer mercy implied the right to punish, a right which had to constantly be reasserted; the assertion of authority was deeply gendered and necessitated the subordination of local men. A case study of ritual humiliation during the conquest of the Caucasus illustrates how this worked in practice: disrupting the customary gender relations of a combatant society to remove opposing men from their authoritative role and installing an imperial officer at the top of the hierarchy.
Structural empowerment, personal initiative, and job satisfaction in service employees: Exploring the mediating role of psychological empowerment
Authors: Orgambídez Alejandro , Benítez Miryam , León‐Pérez Jose M. , Cantero‐Sánchez Francisco J. 
Published: June 2024

IntroductionThis study explores how empowering human resource management (HRM) practices based on structural empowerment (access to opportunities, resources, support, and information) affect both personal initiative and job satisfaction of service employees through individual‐level factors (psychological empowerment).MethodsWe conducted a cross‐sectional survey study and collected 439 valid responses from service employees in Spain. The hypotheses were tested using structural equation modeling (SEM) with confidence intervals based on 10,000 resamples (i.e., bootstrapping technique).ResultsOur results showed that psychological empowerment partially mediated the relationship between structural empowerment and job satisfaction. It also fully mediated the relationship between structural empowerment and personal initiative at work.ConclusionThese findings emphasize the importance of HRM practices that can empower employees as key determinants of job satisfaction and personal initiative at service companies. Furthermore, a structural empowerment approach is a valid theoretical framework for studying and understanding employees' affective evaluations of work and, more specifically, their personal initiative.
The existence of the world as an irrational and “rational” fact
Authors: Cimino Andrea 
Published: June 2024

AbstractThis article reconstructs and defends Husserl's argument for the indubitability of the existence of the world as grounded in ultimate principles. Responding to criticisms about the feasibility of a Husserlian‐informed metaphysical cosmology, it offers a systematic account that explores the question of the world's existence at three distinct levels (factual‐empirical, eidetic, and transcendental), leading to a threefold characterization of the world. First, the obviousness of the world's existence serves as our point of departure. The analysis then moves from a conception of the world as (i) an ontic factum and pregiven ground of all theoretical and practical endeavors in the natural attitude, to a priori ontological considerations of (ii) the essence of “world in general.” However, the insufficiency of both factual‐empirical and a priori investigations necessitates a further displacement of the analysis into the transcendental field of pure phenomena. In accordance with the fundamental principle of synthesis, the world is thus reconceived of as (iii) a phenomenological factum. In this context, the clarification of the existence of the world as both an irrational and a “rational” fact provides all the elements necessary to demonstrate the relative apodicticity and empirical indubitability of the world. This justifies our doxic certainty of the world's existence.
Multi‐scale mechanisms driving root regeneration: From regeneration competence to tissue repatterning
Authors: García‐Gómez Monica L. , ten Tusscher Kirsten 
Published: June 2024

SUMMARYPlants possess an outstanding capacity to regenerate enabling them to repair damages caused by suboptimal environmental conditions, biotic attacks, or mechanical damages impacting the survival of these sessile organisms. Although the extent of regeneration varies greatly between localized cell damage and whole organ recovery, the process of regeneration can be subdivided into a similar sequence of interlinked regulatory processes. That is, competence to regenerate, cell fate reprogramming, and the repatterning of the tissue. Here, using root tip regeneration as a paradigm system to study plant regeneration, we provide a synthesis of the molecular responses that underlie both regeneration competence and the repatterning of the root stump. Regarding regeneration competence, we discuss the role of wound signaling, hormone responses and synthesis, and rapid changes in gene expression observed in the cells close to the cut. Then, we consider how this rapid response is followed by the tissue repatterning phase, where cells experience cell fate changes in a spatial and temporal order to recreate the lost stem cell niche and columella. Lastly, we argue that a multi‐scale modeling approach is fundamental to uncovering the mechanisms underlying root regeneration, as it allows to integrate knowledge of cell‐level gene expression, cell‐to‐cell transport of hormones and transcription factors, and tissue‐level growth dynamics to reveal how the bi‐directional feedbacks between these processes enable self‐organized repatterning of the root apex.
The interplay of homology‐directed repair pathways in the repair of zebularine‐induced DNA–protein crosslinks in Arabidopsis
Authors: Dvořák Tomaštíková Eva , Vaculíková Jitka , Štenclová Veronika , Kaduchová Kateřina , Pobořilová Zuzana , Paleček Jan J. , Pecinka Ales 
Published: June 2024

SUMMARYDNA–protein crosslinks (DPCs) are highly toxic DNA lesions represented by proteins covalently bound to the DNA. Persisting DPCs interfere with fundamental genetic processes such as DNA replication and transcription. Cytidine analog zebularine (ZEB) has been shown to crosslink DNA METHYLTRANSFERASE1 (MET1). Recently, we uncovered a critical role of the SMC5/6‐mediated SUMOylation in the repair of DPCs. In an ongoing genetic screen, we identified two additional candidates, HYPERSENSITIVE TO ZEBULARINE 2 and 3, that were mapped to REGULATOR OF TELOMERE ELONGATION 1 (RTEL1) and polymerase TEBICHI (TEB), respectively. By monitoring the growth of hze2 and hze3 plants in response to zebularine, we show the importance of homologous recombination (HR) factor RTEL1 and microhomology‐mediated end‐joining (MMEJ) polymerase TEB in the repair of MET1‐DPCs. Moreover, genetic interaction and sensitivity assays showed the interdependency of SMC5/6 complex, HR, and MMEJ in the homology‐directed repair of MET1‐DPCs in Arabidopsis. Altogether, we provide evidence that MET1‐DPC repair in plants is more complex than originally expected.
A multicentric intermediate‐size B‐cell lymphoma with epitheliotropism in a Freiberger mare
Authors: Veiga Inês Berenguer , Affolter Verena K. , Moore Peter F. , Maia‐Nussbaumer Päivi de Jesus , Gerber Vincenz , Unger Lucia , Dettwiler Martina 
Published: June 2024

AbstractThis report describes a multicentric intermediate‐size B‐cell lymphoma with epitheliotropism in a Freiberger mare affecting multiple mucous membranes, skin and internal organs. The clonal neoplastic B‐cell population was accompanied by numerous reactive polyclonal small T cells. Differential diagnoses for these unusual findings are discussed.
Dissecting natural hybridisation in longhorned beetles through an integrative approach: Further proof of reticulate evolution in Dorcadionini (Cerambycidae, Lamiinae)
Authors: Caba Florina‐Georgiana , Dascălu Maria‐Magdalena 
Published: June 2024

AbstractThis work demonstrates for the first time using molecular markers alongside chromosomes and the intermediate phenotype that a specimen of Dorcadion is an accidental first‐generation hybrid. The analyses were based on two mitochondrial and five nuclear molecular markers. While analysing the putative parental taxa of the hybrid, it was revealed that even Dorcadion lugubre lugubre Kraatz is likely a hybridogenic taxon. Coincidentally, a new lead was discovered concerning the parental taxa of Dorcadion aethiops (Scopoli), already known as a hybridogenic species. This new evidence of hybridisation adds to the recent publications to strengthen the hypothesis that, in Dorcadionini, this phenomenon is relatively frequent, and speciation is often reticulate: New taxa can appear through introgressive hybridisation, explaining in part the extreme species richness of the group.
Validity of Synchronous Online Physical Education Amidst COVID-19 Pandemic
Authors: Mariano Janet S. , Macapagal Luisito S. 
Published: June 2024

Purpose: Various online physical education activities and methods have been applied to colleges in the Philippines during the COVID-19 confinement. However, much remains unknown about the effects of the physical activity given to students. This study aimed to analyze the validity of online synchronous physical education classes using a progressive cardio workout program among college students. Method: One hundred and ninety-four physical education students enrolled in morning class voluntarily participated. Baseline and posttest measures included resting heart rates, training heart rates, heart rate recovery (HRR), and the YMCA 3-min step test (YMCA 3MST). Results: The progressive cardio workout program over the 8 weeks significantly improved the training heart rate of the participants, X2F(7) = 475.397, p = .001. Also, there is a significant difference between pretest HRR (Mdn = 24) from posttest HRR (Mdn = 16), W = 10,863, p < .001. The pretest HRR was significantly higher than the posttest HRR, the rank–biserial correlation, rB = .586, suggests that this is a large effect size. Further, the pretest YMCA 3MST (Mdn = 120.5) was significantly different from the posttest YMCA 3MST (Mdn = 116), W = 10,946, p < .001, the rank–biserial correlation, rB = .286, suggests that this is a small to medium effect size. Conclusion: This study demonstrated that the 8-week progressive cardio workout program significantly improves the participants’ cardiorespiratory fitness based on the pre- and posttraining heart rate, HRR, and YMCA 3MST results.
Development of a vesicular stomatitis virus pseudotyped with herpes B virus glycoproteins and its application in a neutralizing antibody detection assay
Authors: Kinoshita Hitomi , Yamada Souichi , Ogawa Takuma , Nguyen Phu Hoang Anh , Harada Shizuko , Kawahara Madoka , Ishijima Keita , Maeda Ken , Ebihara Hideki , Fukushi Shuetsu 
Published: June 2024

ABSTRACT

Herpes B virus (BV) is a zoonotic virus and belongs to the genus
Simplexvius
, the same genus as human herpes simplex virus (HSV). BV typically establishes asymptomatic infection in its natural hosts, macaque monkeys. However, in humans, BV infection causes serious neurological diseases and death. As such, BV research can only be conducted in a high containment level facility (i.e., biosafety level [BSL] 4), and the mechanisms of BV entry have not been fully elucidated. In this study, we generated a pseudotyped vesicular stomatitis virus (VSV) expressing BV glycoproteins using G-complemented VSV∆G system, which we named VSV/BVpv. We found that four BV glycoproteins (i.e., gB, gD, gH, and gL) were required for the production of a high-titer VSV/BVpv. Moreover, VSV/BVpv cell entry was dependent on the binding of gD to its cellular receptor nectin-1. Pretreatment of Vero cells with endosomal acidification inhibitors did not affect the VSV/BVpv infection. The result indicated that VSV/BVpv entry occurred by direct fusion with the plasma membrane of Vero cells and suggested that the entry pathway was similar to that of native HSV. Furthermore, we developed a VSV/BVpv-based chemiluminescence reduction neutralization test (CRNT), which detected the neutralization antibodies against BV in macaque plasma samples with high sensitivity and specificity. Crucially, the VSV/BVpv generated in this study can be used under BSL-2 condition to study the initial entry process through gD–nectin-1 interaction and the direct fusion of BV with the plasma membrane of Vero cells.

IMPORTANCE

Herpes B virus (BV) is a highly pathogenic zoonotic virus against humans. BV belongs to the genus
Simplexvius
, the same genus as human herpes simplex virus (HSV). By contrast to HSV, cell entry mechanisms of BV are not fully understood. The research procedures to manipulate infectious BV should be conducted in biosafety level (BSL)-4 facilities. As pseudotyped viruses provide a safe viral entry model because of their inability to produce infectious progeny virus, we tried to generate a pseudotyped vesicular stomatitis virus bearing BV glycoproteins (VSV/BVpv) by modification of expression constructs of BV glycoproteins, and successfully obtained VSV/BVpv with a high titer. This study has provided novel information for constructing VSV/BVpv and its usefulness to study BV infection.
Risk factors and molecular epidemiology of intestinal colonization by carbapenem-resistant Gram-negative bacteria in patients with hematological diseases: a multicenter case‒control study
Authors: Hu Huangdu , Wang Yinping , Sun Jian , Wang Yuting , Zhou Junxin , Shi Qiucheng , Han Xinhong , Jiang Yan , Wu Depei , Huang Xiaojun , Yu Yunsong 
Published: June 2024

ABSTRACT

Patients with hematological diseases are considered to be at high risk for intestinal colonization by carbapenem-resistant Gram-negative bacteria (CR-GNB). However, the epidemiological data regarding risk factors and molecular characteristics of intestinal colonized CR-GNB isolates in this population are insufficient in China. A multicenter case‒control study involving 4,641 adult patients with hematological diseases from 92 hospitals across China was conducted. Following culture of collected rectal swabs, mass spectrometry and antimicrobial susceptibility tests were performed to identify GNB species and CR phenotype. Risk factors were assessed through retrospective clinical information. Whole-genome sequencing was used to analyze the molecular characteristics of CR-GNB isolates. This trial is registered with ClinicalTrials.gov as NCT05002582. Our results demonstrated that among 4,641 adult patients, 10.8% had intestinal colonization by CR-GNB. Of these, 8.1% were colonized by carbapenem-resistant
Enterobacterales
(CRE), 2.6% were colonized by carbapenem-resistant
Pseudomonas aeruginosa
(CRPA), and 0.3% were colonized by carbapenem-resistant
Acinetobacter baumannii
(CRAB). The risk factors for CR-GNB colonization include male gender, acute leukemia, hematopoietic stem cell transplantation, β-lactam antibiotic usage, and the presence of non-perianal infections within 1 week. Compared with CRPA-colonized patients, patients using carbapenems were more likely to be colonized with CRE. NDM was the predominant carbapenemase in colonized CRE. This study revealed a high CR-GNB intestinal colonization rate among adult patients with hematological diseases in China, with CRE being the predominant one. Notably, a significant proportion of CRE exhibited metallo-β-lactamase production, indicating a concerning trend. These findings emphasize the importance of active screening for CR-GNB colonization in patients with hematological diseases.

IMPORTANCE
Carbapenem-resistant Gram-negative bacteria (CR-GNB) has emerged as a significant threat to public health. Patients with hematological diseases are at high risk of CR-GNB infections due to their immunosuppressed state. CR-GNB colonization is an independent risk factor for subsequent infection. Understanding the risk factors and molecular characteristics of CR-GNB associated with intestinal colonization in patients with hematological diseases is crucial for empirical treatment, particularly in patients with febrile neutropenia. However, the epidemiology data are still insufficient, and our study aims to determine the intestinal colonization rate of CR-GNB, identify colonization risk factors, and analyze the molecular characteristics of colonized CR-GNB isolates.
Cohort profile: BioMD-Y (biopsychosocial factors of major depression in youth) – a biobank study on the molecular genetics and environmental factors of depression in children and adolescents in Munich
Authors: Scherff Aline Doreen , Feldmann Lisa , Piechaczek Charlotte , Pehl Verena , Wagenbüchler Petra , Wermuth Inga , Ghotbi Neda , Allgaier Antje-Kathrin , Freisleder Franz Joseph , Beins Eva C , Forstner Andreas J , Nöthen Markus M , Czamara Darina , Rex-Haffner Monika , Ising Marcus , Binder Elisabeth , Greimel Ellen , Schulte-Körne Gerd 
Published: June 2024

PurposeBioMD-Y is a comprehensive biobank study of children and adolescents with major depression (MD) and their healthy peers in Germany, collecting a host of both biological and psychosocial information from the participants and their parents with the aim of exploring genetic and environmental risk and protective factors for MD in children and adolescents.ParticipantsChildren and adolescents aged 8–18 years are recruited to either the clinical case group (MD, diagnosis of MD disorder) or the typically developing control group (absence of any psychiatric condition).Findings to dateTo date, four publications on both genetic and environmental risk and resilience factors (includingFKBP5, glucocorticoid receptor activation, polygenic risk scores, psychosocial and sociodemographic risk and resilience factors) have been published based on the BioMD-Y sample.Future plansData collection is currently scheduled to continue into 2026. Research questions will be further addressed using available measures.