bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transgene copy number distribution profiles in recombinant CHO cell lines revealed by single cell analyses
Authors: He Luhong , Winterrowd Christal , Kadura Ibrahim , Frye Christopher 
Published: January 2012

AbstractClonally derived recombinant cell lines are highly desired to achieve consistent production of recombinant biotherapeutics. Despite repeated rounds of cloning by limiting dilution or single cell cloning, the resulting cell lines have often been observed to diverge, becoming a heterogeneous population and losing productivity over long‐term sub‐culturing. To understand the underlying molecular mechanisms, we developed quantitative polymerase chain reaction (qPCR) assays for the analysis of transgene copy number distribution in single recombinant cells isolated from Chinese hamster ovary (CHO) cell lines. Single cells were obtained by fluorescence activated cell sorting (FACS) technology and lysed directly in 96‐well plates. qPCR assays were then applied to analyze the quantity and distribution of transgenes in those single cells. Results revealed multiple types of transgene copy number distribution profiles from those clonally derived CHO cell lines. The cell lines that maintained productivity over time displayed relatively constant and homogeneous transgene copy number distributions; while most of those cell lines exhibiting a loss of productivity over time showed varying degrees of transgene copy number heterogeneity and distribution drift with passaging. Some cell lines showed the existence of a significant portion of cells lacking the transgenes (referred to as negative cells in this study) and the percentage of those negative cells increased with subsequent generations. Criteria based on transgene copy number distribution profiles were developed to assess cell line suitability for clinical applications, which include (i) percentage of negative cells; (ii) standard deviation of qPCR threshold cycle (Ct) value, a measure of population heterogeneity; (iii) mean Ct changes during aging, a measure of population drift. By implementing these criteria, undesirable cell lines were eliminated for further clinical and commercial applications. Biotechnol. Bioeng. 2012; 109:1713–1722. © 2012 Wiley Periodicals, Inc.
Phidippides Cardiomyopathy: A Review and Case Illustration
Authors: Trivax Justin E. , McCullough Peter A. 
Published: January 2012

AbstractPhidippides was a Greek messenger who experienced sudden death after running more than 175 miles in two days. In today's world, marathon running and other endurance sports are becoming more popular and raising concern about sudden deaths at these events. Once etiologies such has hypertrophic cardiomyopathy, anomalous coronary arteries, and coronary atherosclerosis have been excluded, there is now an additional consideration termed Phidippides cardiomyopathy. Because endurance sports call for a sustained increase in cardiac output for several hours, the heart is put into a state of volume overload. It has been shown that approximately one‐third of marathon runners experience dilation of the right atrium and ventricle, have elevations of cardiac troponin and natriuretic peptides, and in a smaller fraction later develop small patches of cardiac fibrosis that are the likely substrate for ventricular tachyarrhythmias and sudden death. Cardiac magnetic resonance imaging is emerging as the diagnostic test of choice for this condition. This review and case report summarizes the key features of this newly appreciated disorder. © 2012 Wiley Periodicals, Inc.The authors have no funding, financial relationships, or conflicts of interest to disclose.
Triple negative breast cancer is associated with an increased risk of residual invasive carcinoma after lumpectomy
Authors: Sioshansi Shirin , Ehdaivand Shahrzad , Cramer Christina , Lomme Michele M. , Price Lori Lyn , Wazer David E. 
Published: January 2012

AbstractBACKGROUND:To assess the potential mechanisms that may underlie increased local failure in triple negative (TN) breast cancers, an analysis was performed of the risk of residual carcinoma after lumpectomy with correlation to pathologic factors, including molecular phenotype.METHODS:A review of pathologic specimens was performed for women with invasive breast cancer treated with lumpectomy followed by reexcision. Data were collected on age; tumor size, grade, and nodal stage; estrogen receptor, progesterone receptor, and human endothelial growth factor receptor 2 (Her2); extensive intraductal component; lymphovascular invasion; margins; and reexcision findings. Univariate and multivariate logistic regression analyses were performed to evaluate for associations between pathologic features of the lumpectomy specimen and reexcision findings. Molecular phenotypes were defined by conventionally used immunohistochemical pattern.RESULTS:Data were collected on 369 patients with breast cancer. The median age was 57 years, median tumor size was 1.5 cm, 36% had positive margins, 32% had positive lymph nodes, 73.5% had the luminal A subtype, 9.5% had the luminal B subtype, 4.5% were Her2‐enriched, and 12.5% were TN. Overall, 32% of patients had invasive cancer in their reexcision specimens, and 51% of those with the TN subtype had residual invasive disease on reexcision compared with 30% to 31% for other subtypes. On univariate analysis, age, tumor size, margin status, lymphovascular invasion, nodal status, and TN subtype were associated with elevated risk of residual invasive cancer. On multivariate analysis using a forward stepwise model, TN subtype maintained significance, with an odds ratio of 3.28 (P = .002).CONCLUSION:TN subtype has a statistically significant association with an increased risk of residual tumor. This suggests the putative increase in the risk of local failure in TN patients may be related to increased residual tumor burden. Cancer 2012. � 2012 American Cancer Society.
Platinum(IV)‐Mediated Nitrile–Amidoxime Coupling Reactions: Insights into the Mechanism for the Generation of 1,2,4‐Oxadiazoles
Authors: Bolotin Dmitrii S. , Bokach Nadezhda A. , Haukka Matti , Kukushkin Vadim Yu. 
Published: January 2012

AbstractThe nucleophilic addition of amidoximes R′C(NH2)NOH (4: R′=Me, 5: CH2Ph, 6: Ph) to coordinated nitriles in the platinum(IV) complexes trans‐[PtCl4(RCN)2] (1: R=Et, 2: Ph, 3: NMe2) proceeds in a 1:1 molar ratio and leads to the monoaddition products [PtCl4(RCN){HNC(R)ONC(R′)NH2}] (7: R/R′: Et/CH2Ph, 8: Et/Ph, 9: NMe2/CH2Ph, 10: NMe2/Ph). Meanwhile, if the nucleophilic addition proceeds in a 2:1 molar ratio the reaction gives the bisaddition species [PtCl4{HNC(R)ONC(R′)NH2}2] (11: R/R′=Et/Me, 12: Et/CH2Ph, 13: Et/Ph, 14: Ph/Ph, 15: NMe2/Me, 16: NMe2/CH2Ph, 17: NMe2/Ph). All complexes 7–17 bear nitrogen‐bound O‐iminoacylated amidoxime groups. The addition of one equivalent of the corresponding amidoxime to each of 7–10 leads to 12, 13, 16, and 17, respectively. Complex [PtCl4(NCNMe2){HNC(NMe2)ONC(Ph)NH2}] (10), when dissolved in MeNO2, gave mer‐[PtCl3{HNC(NMe2)ONC(Ph)NHC(NMe2)NH}] (18) with the newly formed tridentate ligand derived from an unexpected coupling between two Me2NCN ligands and the N and the O centers of the amidoxime. The O‐imidoylamidoxime compounds R′C(NH2)NOCR(NH) (19–25) were liberated from the corresponding complexes 11–17 by treatment with excess NaCN and these metal‐free species were characterized by 1H and 13C{1H} NMR spectroscopy. The conversion of 19–25 into the 3,5‐substituted 1,2,4‐oxadiazole compounds OaNC(R′)NCb(R)(a–b) (26: R/R′=Me/Et, 27: PhCH2/Et, 28: Ph/Et, 29: Ph/Ph, 30: Me/NMe2, 31: PhCH2/NMe2, 32: Ph/NMe2) occurs at room temperature and the cyclization is promoted by strong acceptor substituents R′.
PAPR reduction scheme with selective tone reservation for OFDM signals
Authors: Chen Chun‐Chyuan , Wu Jiun‐Ming 
Published: January 2012

SUMMARYIn this paper, we propose a peak‐to‐average power ratio (PAPR) reduction scheme with selective tone reservation. In this scheme, the preset peak reduction tone (PRT) set would be divided into M PRT subsets that can generate M time‐domain kernels. The differences of the slopes for the phase responses of the M time‐domain kernels would be considered to design the M PRT subsets in reducing the PAPR of the OFDM transmitted signal. In addition, we can further enhance the PAPR reduction performance by interactively selecting the most appropriate one among the M time‐domain kernels for each OFDM symbol. Simulation results show that the proposed scheme can achieve better PAPR reduction performance than the conventional tone reservation scheme with the same preset PRT set. In comparison with the conventional tone reservation scheme, our PAPR reduction scheme need not send any side information and increase a little bit hardware complexity to improve the PAPR reduction performance. Copyright © 2012 John Wiley & Sons, Ltd.
Identification of putative retinoic acid target genes downstream of mesenchymal Tbx1 during inner ear development
Authors: Monks Dennis C. , Morrow Bernice E. 
Published: January 2012

AbstractBackground: The T‐box transcription factor Tbx1 is expressed in the otic vesicle and surrounding mesoderm of the periotic mesenchyme (POM) during inner ear development. Mesenchymal Tbx1 is essential for inner ear development, with conditional mutants displaying defects in both the auditory and vestibular systems. We have previously reported that mesodermal Tbx1 loss of function mutants (Mest‐KO) have reduced expression of retinoic acid (RA) metabolic genes, Cyp26a1 and Cyp26c1, in the POM, consistent with other studies showing an increase in mesodermal RA reporter expression in Tbx1−/− embryos. However, putative RA effector genes whose expression is altered downstream of increased otic mesenchymal‐epithelial RA signaling have remained elusive. Results: Here we report the identification of 18 retinoic acid responsive genes altered in Mest‐KO conditional mutants by microarray gene profiling. Nine were chosen for biological validation including quantitative RT‐PCR and in situ hybridization (Otor, Mia, Col2a1, Clu, Adm, Myt1, Dlx3, Itgb3, and Itga2b). Conclusion: Here study provides a series of newly identified RA effector genes for inner ear development downstream of mesenchymal Tbx1 that may contribute to the inner ear phenotype observed in Tbx1 loss of function mouse models. Developmental Dynamics 241:563–573, 2012. © 2012 Wiley Periodicals, Inc.
Organocuprate‐Initiated Domino Michael–Intramolecular Aldol Reaction – Application to the Formation of Ring B of the Aglycon of Landomycins
Authors: Bugaut Xavier , Roulland Emmanuel 
Published: January 2012

AbstractAn innovative access to a β,β‐disubstituted Morita–Baylis–Hillman motif, which is a potential intermediate en route to the synthesis of the aglycon of landomycins, is presented. It consists of a finely optimized organocuprate‐initiated domino Michael–intramolecular aldol reaction. The use of TMSCl and DMAP as additives proved essential to ensure the success of this reaction sequence.
International round‐robin study on the Ames fluctuation test
Authors: Reifferscheid G. , Maes H.M. , Allner B. , Badurova J. , Belkin S. , Bluhm K. , Brauer F. , Bressling J. , Domeneghetti S. , Elad T. , Flückiger‐Isler S. , Grummt H.J. , Gürtler R. , Hecht A. , Heringa M.B. , Hollert H. , Huber S. , Kramer M. , Magdeburg A. , Ratte H.T. , Sauerborn‐Klobucar R. , Sokolowski A. , Soldan P. , Smital T. , Stalter D. , Venier P. , Ziemann Chr. , Zipperle J. , Buchinger S. 
Published: January 2012

AbstractAn international round‐robin study on the Ames fluctuation test [ISO 11350, 2012], a microplate version of the classic plate‐incorporation method for the detection of mutagenicity in water, wastewater and chemicals was performed by 18 laboratories from seven countries. Such a round‐robin study is a precondition for both the finalization of the ISO standardization process and a possible regulatory implementation in water legislation. The laboratories tested four water samples (spiked/nonspiked) and two chemical mixtures with and without supplementation of a S9‐mix. Validity criteria (acceptable spontaneous and positive control‐induced mutation counts) were fulfilled by 92–100%, depending on the test conditions. A two‐step method for statistical evaluation of the test results is proposed and assessed in terms of specificity and sensitivity. The data were first subjected to powerful analysis of variance (ANOVA) after an arcsine‐square‐root transformation to detect significant differences between the test samples and the negative control (NC). A threshold (TH) value based on a pooled NC was then calculated to exclude false positive test results. Statistically, positive effects observed by the William's test were considered negative, if the mean of all replicates of a sample did not exceed the calculated TH. By making use of this approach, the overall test sensitivity was 100%, and the test specificity ranged from 80 to 100%. Environ. Mol. Mutagen. 2012. © 2012 Wiley Periodicals, Inc.
Catholic Counselor's Book Shelf
Authors: Sullivan Daniel C. 
Published: January 2012

REPORT WRITING IN PSYCHOLOGY AND PSYCHIATRY: Jack T. Huber, New YorkJUNIOR HIGH SCHOOL GUIDANCE: Mauritz Johnson, William E. Busacker, Fred Q. Bowman, and Brothers, New YorkEMOTIONAL PROBLEMS OF THE STUDENT: Graham B. Blaine, Jr., Charles C. McArthur, et al
Catholic Counselor's Book Shelf
Authors: Daniel C. 
Published: January 2012

PSYCHOLOGY AND RELIGION: Three Joint Symposia from the ACPA‐APA Meetings of 1957, 1958, 1959. American Catholic Psychological Association, Fordham University, New YorkSelected Papers from the ACPA Meetings of 1957, 1958, 1959. New York: American Catholic Psychological AssociationTO LIVE IS CHRIST: Robert W. Gleason, S. J. Sheed and WardTHE INFORMATION SERVICE IN GUIDANCE: Willa Norris, Franklin R. Zeran, Raymond N. Hatch, Rand McNallyWORKING WITH SUPERIOR STUDENTS: THEORIES AND PRACTICES: Bruce Shertzer, Ed.VOCATIONAL MATURITY OF NINTH‐GRADE BOYS: Donald E. Super, Phoebe L. Over‐street, et. al., Monograph 2.
Catholic Counselor's Bookshelf
Authors: DONDERO AUSTIN 
Published: January 2012

GUIDANCE POLICY AND PRACTICE Richard H. MathewsonIT'S YOUR FUTURE. James J. Cribbin, Ph.D.; Reverend William J. McMahon, M.S.; Brother Philip Harris, O.S.F., Ph.D.GUIDING TODAY'S YOUTH. Los Angeles County Public Schools
Catholic Counselor's Bookshelf
Authors: Dondero Brother Austin 
Published: January 2012

Appraising Vocational Fitness By Means Of Psychological Tests. Donald E. Super and John O. Crites. New York: Harper, 1962Psychological Counseling of Adolesscents. Raymond J. Steimel, Editor, Washington: Catholic University of America Press, 1962.Christian Marriage, Rev. Thomas E. Langer, Milwaukee: The Bruce Publishing Company, 1962.
Women's Interests—Fact or Fiction?
Authors: Harmon Lenore W. 
Published: January 2012

The data used in this study were made available through the Center for Interest Measurement Research and the Student Counseling Bureau, University of Minnesota. Computer analysis was provided by the Numerical Analysis Center, University of Minnesota.While attempting to increase the number of A and B+ scores on the women's SVIB by varying the composition of the criterion group on marital status, it was found that the desired effect was achieved by using a different item selection and weighting system from Strong's original one and not by varying the marital status of the criterion group. The experimental scoring keys developed in the study also acted selectively on original scores of B and B—, raising or lowering them, and generally reducing their number. These findings went beyond the original hypotheses and intent of the study and suggested an explanation for the apparent heterogeneity of women's interests and the resulting difficulty in measuring them.
The ethics of creative growth
Authors: KELLY EUGENE W. 
Published: January 2012

Explicitly ethical discussions among guidance and personnel professionals generally involve intraprofessional concerns and the specifying of minimal standards of professional behavior.The author of this article proposes that wider, more challenging, ethical issues should not be treated as special topics within these professions but should be considered crucial parts of the mainstream of ethical thought and practice. This shift would help to make critical moral problems the professional concern of all guidance and personnel workers, not the specialty of only a few.
Re:Views
Authors: Weinrach Stephen G. 
Published: January 2012

WORK, WORK, WORK: Project WERC: Why Not Explore Rewarding Careers? Produced by The South Carolina Educational Television Station, Columbia, 1971, and distributed by APGA.WORK, WORK, WORK: Career Development: Theory and Research, John J. Pietrofesa and Howard Splete.WORK, WORK, WORK: Facilitating Career Development, Strategies for Counselors. Robert C. Reardon and Harman D. Burck, editors.WORK, WORK, WORK: Occupational Information: Where to Get It in Career Education. Robert Hoppock.WORK, WORK, WORK: Career Wheels, produced by Education‐Career Systems and distributed by APGA.WORK, WORK, WORK: The World of Work: A Guide to Choosing a Career. Walter Dean Myers.WORK, WORK, WORK: Finding Your First Job. Carl McDaniels.WORK, WORK, WORK: How To Complete Job Application Forms. C. Todd Strohmenger.WORK, WORK, WORK: Health at Work. Ward Gardner and Peter Taylor.WORK, WORK, WORK: THE MANPOWER CONNECTION—Education and Work. Eli Ginzberg.THEORIES, TOOLS, AND TECHNIQUES: Personality: Theory Assessment and Research. Lawrence A. Pervin.THEORIES, TOOLS, AND TECHNIQUES: Group Techniques for Program Planning: A Guide to Nominal Group and Delphi Processes. Andre L Delbecq, Andrew H. Van de Ven, and David H. Gustafson.THEORIES, TOOLS, AND TECHNIQUES: Interpersonal Conflict Resolution. Alan C. Filley.THEORIES, TOOLS, AND TECHNIQUES: Psychoanalysis and Feminism. Juliet Mitchell.THEORIES, TOOLS, AND TECHNIQUES: The Behavioral Treatment of Sexual Problems: Volume 2, Intensive Therapy. Jack A. Annon.THEORIES, TOOLS, AND TECHNIQUES: When Schools Care: Creative Use of Groups in Secondary Schools. Irving H. Berkovitz, editor.THEORIES, TOOLS, AND TECHNIQUES: Personal and Interpersonal Appraisal Techniques for: Counselors, Teachers, Students. M.A. Kiley.THEORIES, TOOLS, AND TECHNIQUES: The Healing Journey: New Approaches to Consciousness. Claudio Naranjo.THEORIES, TOOLS, AND TECHNIQUES: Kinesics and Context: Essays on Body Motion Communication. Ray L. Birdwhistell.
Re: Views
Authors: Weinrach Stephen G. 
Published: January 2012

Human Resource Development Videotape Series—Life Skills Carkhuff, Robert R., AmherstVideotapes: Helping Model ModuleAttending Skills ModuleResponding Skills ModulePersonalizing Skills ModuleInitiating Skills ModuleThe Case of JerryThe Case of JaneThe Case of MannyRobert R. Carkhuff. The Art of HelpingRobert R. Carkhuff, and Richard M. Pierce. Trainer's Guide: The Art of Helping.Behavioral Counseling (8 16mm color films)Hosford, Ray E.Films: Identifying the ProblemObserving and Recording BehaviorCounseling Techniques: Social ModelingCounseling Techniques: Desensitization——Part IFormulating the Counseling GoalCounseling Techniques: Reinforcement ProceduresCounseling Techniques: Assertive TrainingCounseling Techniques: Self‐as‐a ModelRay E. Hosford, and Louis A. J. M. deVisser. Behavioral Approaches to Counseling: An IntroductionSystematic Videotraining for Beginning Helpers Allen E. Ivey and Norma B.Basic Attending Skills (2 1/2 hours videotape)Basic Influencing Skills (2 1/2 hours, videotape)Microcounseling: An Introduction (1 hour videotape),Rental includes permission to make a copy on one's own equipment.Norman Kagan. Interpersonal Process Recall: A Method of Influencing Human Interaction.Films: Unit 1 Elements of Facilitating CommunicationUnit 2 Affect Simulation, Film 1Film 2Unit 3 Interviewer Recall New Client, Film A‐1On‐going Therapy, Film A‐2College Resident AdvisorCurriculum Director, Film A‐4Unit 4 Inquirer Role and FunctionUnit 5 Client Recall A Mother, Film B‐1Junior High Student, Film B‐2A Couple, Film B‐3Unit 6 Mutual RecallMother and SocialWorker, Film C‐1Physician and Patient, Film C‐2Dating Couple, Film C‐3Health Team, Film C‐4Mother and Two Children, Film C‐5Intact Family, Film C‐6Classroom Team, Film C‐7Unit 7 IPR Theory—DiscussionNorman Kagan, Interpersonal Process Recall: A Method of Influencing Human Interaction
Re: Views
Authors: Weinrach Stephen G. 
Published: January 2012

Career Decisions Game. Tony Plaskanos and Sam Savitz. Paoli, Pa.: Instructo/McGraw‐Hill, 1976. $10.95.People on the Job. Betty Medsger. Posters. Oakland, Calif.: Mind Openers, 1977. $12 for set of 20.Say It with Puppets. Dayton, Ohio: Pflaum, 1975. $18.95.Mental Retardation and Human Sexuality. Winifred Kempton, Ruth Faden, and Joseph Lascalzo. 20‐minute filmstrip and record. Philadelphia: Planned Parenthood Association of Southeastern Pennsylvania. $25.00.Lifeline. Schools Council Moral Education Curriculum Program (United Kingdom). Kit—Flash cards and user's guide. Niles, Ill.: Argus Communications, 1975. $47.50 for kit, or parts may be purchased separately: Sensitivity Cards, $10.00; Consequence Cards, $10.00; Points of Review Cards, $10.00; Proving the Rule booklets (set of five), $7.50; What Would You Have Done? booklets (set of six), $6.50; Learning to Care: Teacher's Guide, $10.00.A Dialogue on Education and the Control of Human Behavior: Carl R. Rogers and B. F. Skinner. Gerald A. Gladstein, Editor. Six‐cassette album. New York: Jeffrey Norton, 1976. $48.00.Death and Dying: Closing the Circle. Robert Jay Lifton, Consultant. Film strips and cassettes or LP records. New York: Guidance Associates, 1975. Three parts, approximately 16 minutes each. (Series expanded to five parts in 1977; first three are reviewed.) $64.50.Talking Library of Occupational Information for the Blind. Benjamin J. Pumo, Editor. Cassettes. Detroit: Greater Detroit Society for the Blind, 1976. $85.00 plus $4.00 for handling.The Job Game. Cassettes and workbooks. New York: Employment Training Corp., 1975. $149.00.LOOK (Learning Occupational Objectives Kit). Moravia: Chronicle Guidance, 1975. $149.00.Business of Your Own. Three cassettes and illustrated books. Carson, Calif.: Educational Insights, 1976. $150.00.Expo 10—Exploring Career Interest. Judith L. Anderson, Patricia Schildknecht, and John Steuber. Print and nonprint games. Chicago: Science Research Associates. 1975. $385.33 list; $289.00 school price.The World of Work. Cassettes, student‐record booklets, and leader guides. New York: Educational Design, Educational Resources Div., 1969–75. $535.50.A Career for Tomorrow. Audiovideo cartridges. Columbus, Ga.: American Learning Systems, 1975–76. 60 cartridges, 15 to 20 minutes each. $4,600.00 for cartridges; $325.00 to $398.00 for projector.
Re:Views
Authors: Weinrach Stephen G. 
Published: January 2012

Human Development: Child development: An introduction. Robert F. Biehler.Human Development: Concepts and Theories of Human Development. Richard M. Lerner.Human Development: The Psychology of Adolescence. John E. HorrocksHuman Development: Emotional Problems of Adolescents. J. Roswell Gallagher and Herbert I. Harris (3rd ed.).Human Development: CIRCUS, Form A. Reading, Mass.Human Development: From Childhood to Adolescence. Maria Montessori.Human Development: Child Adolescent Psychology (2nd ed.). Gene R. Medinnus and Ronald C. Johnson.Human Development: Developmental Education and Other Emerging Alternatives In Secondary Guidance Programs. G. Dean Miller, Editor.New Instructional Materials: Practical Counseling in the Schools. Gary S. Belkin.New Instructional Materials: Counseling Theory and Process. James C. Hansen, Richard R. Stevic and Richard W. Warner, Jr. (2nd ed.).New Instructional Materials: Ethical Standards Casebook. Robert Callis, Editor (2nd ed.).New Instructional Materials: Fundamentals of Guidance. Bruce Shertzer and Shelly C. Stone (3rd ed.).New Instructional Materials: Guidance in Today's Schools. Donald G. Mortensen and Alan M. Schmuller. (3rd ed.).New Instructional Materials: All Kinds of Weather Friend. Dwight Webb.