bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of capsazepine on [Ca2+]i in MDCK renal tubular cells
Authors: Tsai Jeng‐Yu , Kuo Chun‐Chi , Chou Chiang‐Ting , Chao David , Cheng He‐Hsiung , Wang Jue‐Long , Cheng Jin‐Shiung , Lin Ko‐Long , Huang Jong‐Khing , Chang Hong‐Tai , Jan Chung‐Ren 
Published: December 2010

AbstractThe current study explored whether capsazepine changed basal cytosolic free Ca2+ concentrations ([Ca2+]i) levels in suspended Madin Darby canine kidney (MDCK) cells cells by using fura‐2 as a Ca2+‐selective fluorescent dye. At concentrations of 10–200 µM, capsazepine increased [Ca2+]i in a concentration‐dependent manner. The Ca2+ signal was partially reduced by 40% by removing extracellular Ca2+. Capsazepine induced Mn2+ quench of fura‐2 fluorescence, indirectly implicating Ca2+ entry. Capsazepine‐induced Ca2+ influx was unchanged by L‐type Ca2+ entry inhibitors and protein kinase C modulators [phorbol 12‐myristate 13‐acetate (PMA) and GF109203X]. In Ca2+‐free medium, 100 µM capsazepine‐induced Ca2+ release was substantially suppressed by pretreatment with thapsigargin (an endoplasmic reticulum Ca2+ pump inhibitor). Pretreatment with capsazepine nearly abolished thapsigargin‐induced Ca2+ release. Inhibition of phospholipase C with U73122 did not change capsazepine‐induced [Ca2+]i rises. Collectively, in MDCK cells, capsazepine induced [Ca2+]i rises by causing phospholipase C‐independent Ca2+ release from the endoplasmic reticulum and Ca2+ influx via non‐L‐type Ca2+ channels. Drug Dev Res 72: 323–329, 2011. © 2010 Wiley‐Liss, Inc.
Temporally‐ and spatially regulated generation of distinct descendants by sonic hedgehog‐expressing progenitors in the forebrain
Authors: Wada Yukiko , Yamauchi Kenta , Murakami Fujio , Tanabe Yasuto 
Published: December 2010

AbstractThe generation of distinct neural subtypes depends on the activities of cell‐extrinsic and ‐intrinsic factors during the development of the vertebrate CNS. Previous studies have provided a molecular basis for how neural progenitors are patterned and generate distinct descendants that are spatially and temporally regulated by inductive signals secreted by polarized sources. However, it still remains unknown how the generation of neural descendants by progenitors located at polarized sources of inductive signals is controlled. Sonic hedgehog (Shh), which is expressed at the ventral midline in the forebrain, has been shown to play a critical role for the patterning and specification of distinct neural subtypes in the forebrain. Here, we analyzed the identities and distributions of Shh‐descendants generated at discrete time points in the forebrain by using a ShhcreERT2 mouse driver line in which a tamoxifen‐inducible Cre cassette was inserted into the Shh locus together with a Z/EG mouse reporter line. Our results showed that Shh‐expressing neural progenitors generated neuronal and glial descendants distributed throughout the telencephalon and diencephalon in a temporally distinct manner. Furthermore, our results showed that Shh‐progenitors are located at two spatially distinct sub‐domains that can be characterized by their temporally distinct patterns of Shh expression. These results suggest that temporally‐ and spatially controlled mechanisms that specify neural subtypes operate in the Shh‐expressing progenitor domain, and raise the possibility that the distinct temporal gradient of Shh activity might be responsible for the generation of distinct neural subtypes in the telencephalon. © 2010 Wiley Periodicals, Inc. Develop Neurobiol, 2012
A New Tris(phosphonomethyl) Monoacetic Acid Cyclam Derivative: Synthesis, Acid‐Base and Metal Complexation Studies
Authors: Lima Luís M. P. , Delgado Rita , Plutnar Jan , Hermann Petr , Kotek Jan 
Published: December 2010

AbstractA new cyclam‐based ligand, [4,8,11‐tris(phosphonomethyl)‐1,4,8,11‐tetraazacyclotetradec‐1‐yl]acetic acid (H7te3p1a) was synthesised and characterised. The potentiometricand 31P NMR spectroscopic titrations of the complexes inan aqueous solution at an ionic strength of 0.10 or0.50 mol dm–3 in [N(CH3)4]NO3, respectively, indicated that the ligand is highly basic. The stepwise protonations were dominated by extensive proton relocations and were influenced by the intramolecular hydrogen bonds. The thermodynamic stability constants for the complexes formed by H7te3p1a in the presence of metal ions were determined by potentiometric titrations. The Cu2+ complexes exhibited very high stability, those of Zn2+, Cd2+, Pb2+, La3+, Sm3+, Gd3+, Ho3+ and Lu3+ presented high stability while that of Ca2+ had lower stability, as expected. The ligand and its complexes behaviour is similar to that of the tetrakis(phosphonomethyl)cyclam derivative, H8tetp. The NMR spectroscopic studies of the Zn2+ and Cd2+ complexes pointed to the involvement of the three phosphonate moieties in the coordination of the latter ion, while several isomer species were generally present in solution for the Zn2+ complex. A major isomer was stabilised in solution upon heating. In this isomer only one of the methylphosphonate arms was coordinated to the metal centre and this isomer probably adopts a distorted square pyramidal geometry with the macrocyclic ring in the type I conformation.
Crystal Structures and Physical Properties of Three New Manganese‐Based Coordination Polymers withp‐Biphenyldicarboxylic Acid Linkers
Authors: Jørgensen Mads R. V. , Clausen Henrik F. , Christensen Mogens , Poulsen Rasmus D. , Overgaard Jacob , Iversen Bo B. 
Published: December 2010

AbstractSolvothermal synthesis with manganese nitrate,p‐biphenyldicarboxylic acid, and three different solvents resulted in three new metal–organic framework materials: Mn(C14H8O4)(C3H7NO)2/3(1), Mn2(C14H8O4)3/2(CHO2) (2), and Mn3(C14H8O4)3(C4H9NO)4(3). Two of the syntheses gave multiphase products, but one resulted in a phase‐pure product2. The crystal structures of1and3were solved by using conventional single‐crystal X‐ray diffraction, whereas the small crystal size of2made use of intense synchrotron radiation necessary. The crystal structure of1is composed of layers, which are only held together by van der Waals forces between the solvent of one layer and the framework of the adjacent layers, and the compound decomposes upon exposure to air. Structures2and3are 3D frameworks. Phase‐pure compound2does not contain the expected solvent diethylformamide, but formic acid residues. Measurements of magnetization and heat capacity data show that2is a metastable material with irreversible phase transitions at 6.5 or 4.5 K. In the metastable state,2is antiferromagnetic. Compound3has two types of pores along thebaxis, and solvent molecules occupy the channels. Minor disorder of the solvent molecule is observed in one of the channels.
Particle size distribution in natural water via density estimation
Authors: Jarnicka Jolanta 
Published: December 2010

AbstractIn this paper we use density estimation to investigate the particle size distribution in surface water and to extract the components of density functions obtained. This distribution helps to investigate quality of water with respect to its physical characterization. The analysis is conducted using the two‐step density estimation method. The first step of the method is based on parametric density estimation and uses a modification of the EM algorithm to a normal mixture model. In the second step we apply the generalized kernel density estimator, being the convex combination of the well‐known kernel density estimators.
Carer's experience and perceived effects of a skills based training programme for families of people with eating disorders: A qualitative study
Authors: Macdonald Pam , Murray Joanna , Goddard Elizabeth , Treasure Janet 
Published: December 2010

AbstractPurposeThe study examined the experience of carers of people with eating disorders after having participated in a skills‐based training programme that incorporated five psycho‐educational DVDs and book. Supplemental telephone coaching was also offered.MethodNineteen interview transcripts were analysed using Interpretative Phenomenological Analysis (IPA).Results(1) The transfer of specialist skills was highly valued in lessening carer stress and care‐giving difficulties and, in some instances, appeared to have a positive effect on both carer and sufferer, (2) caregivers provided useful suggestions for building upon the current intervention, (3) future programmes would benefit from stronger fidelity to the principles of motivational interviewing, stricter monitoring of coaches and increased participation in male carers.ConclusionThis study has confirmed that skills‐training can be beneficial in increasing carer self‐efficacy and reducing psychological distress, anxiety and burden. They can be a cost‐effective method of supporting carers who might otherwise struggle to find therapeutic family support. Copyright © 2010 John Wiley & Sons, Ltd and Eating Disorders Association.
Measuring self‐report obsessionality in anorexia nervosa: Maudsley obsessive–compulsive inventory (MOCI) or obsessive–compulsive inventory‐revised (OCI‐R)?
Authors: Roberts Marion , Lavender Anna , Tchanturia Kate 
Published: December 2010

AbstractSelf‐report measures are often used in research and clinical practise as they efficiently gather a large amount of information. With growing numbers of self‐report measures available to target single constructs, it is important to revisit one's choice of instrument to be sure that the most valid and reliable measure is employed. The Maudsley Obsessive–Compulsive Inventory (MOCI) and the Obsessive–Compulsive Inventory‐Revised (OCI‐R) were administered to 223 female participants: 30 inpatients with anorexia nervosa (AN), 62 community cases with AN, 69 community cases weight restored from AN and 62 healthy controls. Both measures distinguished between clinical and healthy groups; however, the OCI‐R showed superior internal reliability. Additionally, the OCI‐R measures six (to the MOCI's four) obsessive–compulsive constructs, and uses a more sensitive response format (likert scale vs. categorical). It is recommended that the OCI‐R be employed as the self‐report instrument of choice for assessing obsessive–compulsive pathology in those with AN. Copyright © 2010 John Wiley & Sons, Ltd and Eating Disorders Association.
Cognitive behavioural therapy for individuals with longstanding anorexia nervosa: Adaptations, clinician survival and system issues
Authors: Bamford Bryony H. , Mountford Victoria A. 
Published: December 2010

AbstractDespite recent advances in the treatment of anorexia nervosa, some individuals will progress to a severe and enduring illness, with associated physical, psychological and social consequences. Working with these patients, however, may leave clinicians feeling overwhelmed, risking difficulties in the therapeutic relationship including disengagement or despair. Cognitive behaviour therapy has shown some promise in the treatment of eating disorders, yet some features may not be appropriate for this group. In this paper, we outline the ways in which we have adapted cognitive behaviour therapy to best meet the complex and challenging needs of this group. We stress the importance of maintaining a reassuring, accepting and motivational approach in combination with clear goal setting and boundaries. Copyright © 2010 John Wiley & Sons, Ltd and Eating Disorders Association.
Heart rate variability as a measure of cardiac autonomic function in anorexia nervosa: A review of the literature
Authors: Mazurak Nazar , Enck Paul , Muth Eric , Teufel Martin , Zipfel Stephan 
Published: December 2010

AbstractObjectivePatients with anorexia nervosa (AN) exhibit a wide range of autonomic system disturbances; these patients have also high mortality risk due to cardio‐vascular complications. Origin and pathogenesis of such changes are not absolutely clear.MethodsRelevant publications were drawn from PUBMED using the keywords ‘anorexia nervosa’ AND ‘autonomic’. Fifty two abstracts were identified and screened for papers that measured the state of autonomic regulation by means of analysis of heart rate variability either during 24‐hour electrocardiography (ECG) monitoring or during a short‐term laboratory test. Studies selected were analysed for the number of patients included, the presence and quality of control groups, gender, age and body mass index (BMI) of patients, type of AN as well as methods used to determine heart rate variability (HRV).ResultsTwenty papers on HRV in patients with anorexia were identified and analysed, revealing three distinct positions regarding changes of autonomic nervous system (ANS) functions in patients with AN. The majority of papers identified parasympathetic/sympathetic imbalance with parasympathetic dominance and decreased sympathetic modulation; others could not replicate these findings, but instead described sympathetic dominance; finally a group of papers could not identify any autonomic differences in comparison to control samples. We conclude that in its current state of analysis HRV may not be suitable for routine assessment of ANS function in AN patients but rather remains a research tool. Copyright © 2010 John Wiley & Sons, Ltd and Eating Disorders Association.
Pyrethroid sorption to Sacramento River suspended solids and bed sediments
Authors: Fojut Tessa L , Young Thomas M 
Published: December 2010

Abstract
Sorption of pyrethroid insecticides to solid materials will typically dominate the fate and transport of these hydrophobic compounds in aquatic environments. Batch reactor isotherm experiments were performed with bifenthrin and λ-cyhalothrin with suspended material and bed sediment collected from the Sacramento River, California, USA. These batch reactor experiments were performed with low spiking concentrations and a long equilibration time (28 d) to be more relevant to environmental conditions. Sorption to suspended material and bed sediment was compared to examine the role of differential sorption between these phases in the environmental transport of pyrethroids. The equilibrium sorption data were fit to the Freundlich isotherm model and fit with r2 &gt; 0.87 for all experiments. Freundlich exponents ranged from 0.72 ± 0.19 to 1.07 ± 0.050, indicating sorption nonlinearity for some of the experimental conditions and linearity for others over the concentration range tested. The Freundlich capacity factors were larger for the suspended solids than for the bed sediments, and the suspended material had a higher specific surface area and higher organic carbon content compared to the bed sediment. Calculated organic carbon-normalized distribution coefficients were larger than those previously reported in the literature, by approximately an order of magnitude, and ranged from 106.16 to 106.68 at an equilibrium aqueous concentration of 0.1 µg/L. Higher than expected sorption of pyrethroids to the tested materials may be explained by sorption to black carbon and/or mineral surfaces. Environ. Toxicol. Chem. 2011; 30:787–792. © 2010 SETAC
Enhanced adsorption of humic acids on ordered mesoporous carbon compared with microporous activated carbon
Authors: Liu Fengling , Xu Zhaoyi , Wan Haiqin , Wan Yuqiu , Zheng Shourong , Zhu Dongqiang 
Published: December 2010

Abstract
Humic acids are ubiquitous in surface and underground waters and may pose potential risk to human health when present in drinking water sources. In this study, ordered mesoporous carbon was synthesized by means of a hard template method and further characterized by X-ray diffraction, N2 adsorption, transition electron microscopy, elemental analysis, and zeta-potential measurement. Batch experiments were conducted to evaluate adsorption of two humic acids from coal and soil, respectively, on the synthesized carbon. For comparison, a commercial microporous activated carbon and nonporous graphite were included as additional adsorbents; moreover, phenol was adopted as a small probe adsorbate. Pore size distribution characterization showed that the synthesized carbon had ordered mesoporous structure, whereas the activated carbon was composed mainly of micropores with a much broader pore size distribution. Accordingly, adsorption of the two humic acids was substantially lower on the activated carbon than on the synthesized carbon, because of the size-exclusion effect. In contrast, the synthesized carbon and activated carbon showed comparable adsorption for phenol when the size-exclusion effect was not in operation. Additionally, we verified by size-exclusion chromatography studies that the synthesized carbon exhibited greater adsorption for the large humic acid fraction than the activated carbon. The pH dependence of adsorption on the three carbonaceous adsorbents was also compared between the two test humic acids. The findings highlight the potential of using ordered mesoporous carbon as a superior adsorbent for the removal of humic acids. Environ. Toxicol. Chem. 2011; 30:793–800. © 2010 SETAC
Comparison of acute and chronic toxicity of silver nanoparticles and silver nitrate toDaphnia magna
Authors: Zhao Chun-Mei , Wang Wen-Xiong 
Published: December 2010

AbstractSilver nanoparticles (AgNP) are now widely used as antibacterial products, and their potential toxicities in aquatic organisms are a matter of increasing concern. In the present study, we conducted experiments to reveal the acute and chronic toxicities of AgNP and its bioaccumulation from both aqueous and dietary sources in a model freshwater cladoceran, Daphnia magna. No mortality was observed in 48-h acute toxicity testing when the daphnids were exposed up to 500 µg Ag/L as AgNP. The AgNP accumulation reached as high as 22.9 mg Ag/g dry weight at the highest AgNP concentration tested (500 µg/L). In contrast, D. magna was extremely sensitive to free Ag ion (Ag+, added as AgNO3), with a measured 48-h 50% lethal concentration of 2.51 µg/L. Thus, any AgNP potential acute toxicity may be caused by the release of Ag+ into the solution. During the 21-d chronic exposure, dietborne AgNO3 had the most significant influence on reproduction, whereas waterborne AgNP had the most significant inhibition on growth. Significant delay and decrease of reproduction in daphnids exposed to dietborne AgNO3 occurred at a dissolved Ag concentration of 0.1 µg/L added to the algae. Significant inhibitions of growth and reproduction were also found for the AgNP exposure, with the lowest observed effective concentration of 5 µg/L and 50 µg/L, respectively. Chronic effects of AgNP were probably caused by the low food quality of algae associated with AgNP and the low depuration of ingested AgNP. Environmental risk assessments of AgNP should therefore include tests on the chronic toxicity to aquatic organisms as well as the direct and indirect effects of AgNP resulting from the release of Ag+ into the environment. Environ. Toxicol. Chem. 2011; 30:885–892. © 2010 SETAC
Development of a polydimethylsiloxane film-based passive dosing method in the in vitro DR-CALUX® assay
Authors: Booij Petra , Lamoree Marja H , Leonards Pim E.G , Cenijn Peter H , Klamer Hans J.C , van Vliet L. Alexander , Åkerman Johan , Legler Juliette 
Published: December 2010

Abstract
In bioassays, exposure concentrations of test compounds are usually expressed as nominal concentrations. As a result of various processes, such as adsorption, degradation, or uptake, the actual freely dissolved concentration of the test compound may differ from the nominal concentration. The goal of the present study was to develop a method to dose passively the freely dissolved fraction of organic chemicals in an in vitro bioassay with adherent cells. To this end, a polydimethylsiloxane (PDMS) film-based method was developed for a reporter gene assay for dioxin-like compounds in a rat liver cell line. Polydimethylsiloxane films loaded with test compounds ensure that the concentration during exposure is in equilibrium and that the ratio between the concentration on the film and the concentration in medium is constant. Benzo[k]fluoranthene (BkF) was used as a model compound to develop the passive dosing method in transwell plates, which was further tested with a complex mixture, i.e., an extract prepared from a contaminated sediment. A higher dioxin-like activity was found when extracts were dosed by passive dosing with PDMS than when directly added to medium. Comparison with analysis of the concentration of BkF in medium shows that passive dosing of individual chemicals may not be necessary if freely dissolved concentrations are known. Use of PDMS for passive dosing of complex samples may represent a more realistic method for exposure in in vitro bioassays. Environ. Toxicol. Chem. 2011; 30:898–904. © 2010 SETAC
Distributional Kalman filters for Bayesian forecasting and closed form recurrences
Authors: Smith Jim Q. , Freeman G. 
Published: December 2010

AbstractOver the last 50 years there has been an enormous explosion in developing full distributional analogues of the Kalman filter. In this paper we explore how some of the second‐order processes discovered by Kalman have their analogues in Bayesian state space models. Many of the analogues in the lierature need to be calculated using numerical methods like Markov chain Monte Carlo so they retain, or even enhance, the descriptive power of the Kalman filter, but at the cost of reduced transparency. However, if the analogues are drawn properly, elegant recurrence relationships—like those of the Kalman filter—can still be developed that apply, at least, for one‐step‐ahead forecast distributions. In this paper we explore the variety of ways such models have been built, in particular with respect to graphical time series models. Copyright © 2010 John Wiley & Sons, Ltd.
A robust model of the convenience yield in the natural gas market
Authors: Volmer Thomas 
Published: December 2010

AbstractThis study advances the research on the convenience yield of natural gas. Econometric models confirm that air temperature is an important explanatory variable in addition to storage levels. Furthermore, an extended linear model shows that one has to account for a changing cost of physical storage in the spirit of Brennan (1958). Besides this, an alternative regime‐switching model for the convenience yield helps to put in perspective a prominent finding by Fama, and French (1987). That is, given binding capacity constraints for gas storage, the variance of the futures' basis will increase rather than decrease with the storage levels. Finally and most importantly, robustness tests demonstrate that the extended linear model produces the most viable forecasts and that these forecasts can help to amend the performance of reduced‐form models for the gas spot price. © 2010 Wiley Periodicals, Inc. Jrl Fut Mark
On approximating deep in‐the‐money Asian options under exponential Lévy processes
Authors: Tchuindjo Leonard 
Published: December 2010

AbstractThis note proposes a new approach of valuing deep in‐the‐money fixed strike and discretely monitoring arithmetic Asian options. This new approach prices Asian options whose underlying asset price evolves according to the exponential of a Lévy process as a weighted sum of European options. Numerical results from experimenting on three different types of Lévy processes—a diffusion process, a jump diffusion process, and a pure jump process—illustrate the accuracy of the approach. © 2010 Wiley Periodicals, Inc. Jrl Fut Mark