bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blood purification therapy in chronic renal failure and its impact on renal index, serological index, and inflammatory factors
Authors: Butt Batool , Mushtaq Adnan , Hameed Fatima Abdul , Abbasi Muhammad Sajid Rafique , Tariq Maham , Akbar Amna , Jadoon Sarosh Khan , Tasneem Sabahat , Ahmad Mumtaz , Zeb Anam , Alvi Sarosh 
Published: May 2024

Introduction:
This study aimed to explore the clinical effects of blood purification therapy in patients with chronic renal disease, measured by renal function index and inflammation.

Methodology:
Data were collected from a tertiary care hospital in Pakistan between June 2022 and September 2023. Eighty-four patients undergoing maintenance hemodialysis for chronic renal failure were retrospectively included in this cohort.

Results:
Age, sex, BMI, course of disease, primary disease, and educational level were not related to the response to blood purification treatment. Blood purification therapy positively affected renal function, serological indices, and inflammatory factors (P<0.05).

Conclusion:
Blood purification therapy can improve toxin clearance and renal function and reduce inflammation. Therefore, the authors can conclude that this is an effective therapy for our population.
Countering Calcium Blooming With Personalized Contrast Media Injection Protocols
Authors: McDermott Michael C. , Sartoretti Thomas , Stammen Lion , Martens Bibi , Jost Gregor , Pietsch Hubertus , Gutjahr Ralf , Schmidt Bernhard , Flohr Thomas G. , Alkadhi Hatem , Wildberger Joachim E. 
Published: May 2024

Objective
Photon-counting detector computed tomography (PCD-CT) enables spectral data acquisition of CT angiographies allowing for reconstruction of virtual monoenergetic images (VMIs) in routine practice. Specifically, it has potential to reduce the blooming artifacts associated with densely calcified plaques. However, calcium blooming and iodine attenuation are inversely affected by energy level (keV) of the VMIs, creating a challenge for contrast media (CM) injection protocol optimization. A pragmatic and simple rule for calcium-dependent CM injection protocols is investigated and proposed for VMI-based coronary CT angiography with PCD-CT.

Materials and Methods
A physiological circulation phantom with coronary vessels including calcified lesions (maximum CT value >700 HU) with a 50% diameter stenosis was injected into at iodine delivery rates (IDRs) of 0.3, 0.5, 0.7, 1.0, 1.5, 2.0, 2.5, and 3.0 g I/s. Images were acquired using a first-generation dual-source PCD-CT and reconstructed at various VMI levels (between 45 and 190 keV). Iodine attenuation in the coronaries was measured at each IDR for each keV, and blooming artifacts from the calcified lesions were assessed including stenosis grading error (as % overestimation vs true lumen). The IDR to achieve 300 HU at each VMI level was then calculated and compared with stenosis grading accuracy to establish a general rule for CM injection protocols.

Results
Plaque blooming artifacts and intraluminal iodine attenuation decreased with increasing keV. Fixed windowing (representing absolute worst case) resulted in stenosis overestimation from 77% ± 4% at 45 keV to 5% ± 2% at 190 keV, whereas optimized windowing resulted in overestimation from 29% ± 3% at 45 keV to 4% ± 1% at 190 keV. The required IDR to achieve 300 HU showed a strong linear correlation to VMI energy (R
2 = 0.98). Comparison of this linear plot versus stenosis grading error and blooming artifact demonstrated that multipliers of 1, 2, and 3 times the reference IDR for theoretical clinical regimes of no, moderate, and severe calcification density, respectively, can be proposed as a general rule.

Conclusions
This study provides a proof-of-concept in an anthropomorphic phantom for a simple pragmatic adaptation of CM injection protocols in coronary CT angiography with PCD-CT. The 1-2-3 rule demonstrates the potential for reducing the effects of calcium blooming artifacts on overall image quality.
Surgical Treatment Strategies for Residual or Recurrent Intracranial Aneurysms Following Endovascular Embolization
Authors: Zhao Haijun , Gao Kaiming , Shi Minggang , Shang Yanguo , Tong Xiaoguang 
Published: May 2024

Objective:
To explore how to effectively manage the residual or recurrent intracranial aneurysms after embolization.

Methods:
The authors retrospectively reviewed our experience of endovascular interventional therapy, surgical clipping, and cerebrovascular bypass surgery in the treatment of residual or recurrent aneurysms after embolization at the authors’ institution from 2018 to 2022.

Results:
The Glasgow Outcome Scale of 28 patients after the procedure and at discharge showed that 24 recovered well, 3 had severe disability, and 1 died. During the 24-month follow-up, 26 had a good recovery, 1 suffered from disability, and 1 died. Two cases of aneurysm recurrence were detected, and both were treated through endovascular therapy. Among them, 1 case underwent a repeat endovascular embolization, and 1 case was switched to surgical clipping. No residual aneurysms were observed in the remaining patients who underwent bypass surgery, and their bypass grafts were all patent.

Conclusion:
Based on the clinical status of patients, aneurysmal characteristics, surgical risk, and possibility of rerupture of aneurysms, an individualized strategy was proposed for residual or recurrent aneurysms after embolization. The use of endovascular interventional therapy or surgical clipping can be safely and effectively managed, and cerebrovascular bypass surgery can effectively manage complex aneurysms.
Validating the Supraorbital Notch-Nasion-Supraorbital Notch Angle as a Marker for Metopic Craniosynostosis Severity
Authors: Romeo Dominic J. , Becerra Santiago Lopez , Barrero Carlos E. , Massenburg Benjamin B. , Du Steven , Ng Jinggang J. , Wu Meagan , Swanson Jordan W. , Taylor Jesse A. , Bartlett Scott P. 
Published: May 2024

Objective:
Metopic craniosynostosis (MCS) can be difficult to differentiate from metopic ridge (MR) or normal frontal morphology. This study assess whether the supraorbital notch-nasion-supraorbital notch (SNS) angle can help identify MCS.

Methods:
Records of 212 patients with preoperative three-dimensional computed tomography scans were examined. The SNS angles, surgeon craniofacial dysmorphology rankings, and CranioRate metopic severity scores (MSSs) were compared with the Spearman rank correlation coefficient. Receiver operating characteristic (ROC) curves with Youden J-statistic and cross-validation of regression models assessed the ability of these measures to predict surgery.

Results:
A total of 212 patients were included, consisting of 78 MCS, 37 MR, and 97 controls. Both the mean SNS angle (MCS: 111.7 ± 10.7 degrees, MR: 126.0 ± 8.2 degrees, controls: 130.7 ± 8.8 degrees P < 0.001) and MSS (MCS: 5.9 ± 2.0, MR: 1.4 ± 1.9, controls: 0.2 ± 1.9, P < 0.001) were different among the cohorts. The mean SNS angle (111.5 ± 10.7 versus 129.1 ± 8.8, P < 0.001) was lower in those who had surgery and CranioRate score (5.9 ± 2.1 versus 0.8 ± 2.2, P < 0.001) was higher in those who underwent surgery. SNS angles were positively correlated with surgeon craniofacial dysmorphology rankings (r = 0.41, P < 0.05) and CranioRate MSS (r = 0.54, P < 0.05). The ROC curve requiring high sensitivity revealed an SNS angle of 124.8 degrees predicted surgery with a sensitivity of 88.7% and a specificity of 71.3%. A ROC curve using the CranioRate MCC values ≥3.19 predicted surgery with 88.7% sensitivity and 94.7% specificity.

Conclusion:
Orbital dysmorphology in patients with MCS is well captured by the supraorbital-nasion angle. Both the SNS angle and CranioRate MSS scores accurately predict surgical intervention.
Characterizing Vitamin B12 Deficiency in Neurology Outpatients: A Retrospective Observational Study
Authors: Zhou Li , Bai Xue , Wu Bowen , Tan Yongjun , Li Mengxia , Yang Qin 
Published: May 2024

Objectives
Clinical manifestations of vitamin B12 deficiency are varied and may result in missed or delayed diagnosis. This investigation explores the diverse clinical manifestations and demographic characteristics of vitamin B12 deficiency in neurology outpatients, aiming to enhance timely diagnosis and outcomes.

Methods
The severity of vitamin B12 deficiency was classified as absolute (≤150 pg/mL) or borderline deficiency (150–300 pg/mL). We conducted a retrospective analysis of 165 outpatients with vitamin B12 deficiency at the department of neurology between May 2020 and May 2021.

Result
Absolute vitamin B12 deficiency was found in 23.0% of the patients. The most common age range was 50–60 years, the most common cause was vegetarianism, and the most common symptom was headache. Epileptiform symptoms were more likely to occur in younger patients (<20 years old) with vitamin B12 deficiency, whereas psychiatric symptoms were more likely to occur in older patients (>70 years old). Vegetarians, salivation, and nonmegaloblastic anemia were more obvious in patients with absolute vitamin B12 deficiency, whereas headaches often showed borderline B12 deficiency.

Conclusions
The clinical characteristics of vitamin B12 deficiency are complex and nonspecific. The diagnosis should be based on multiple factors.
Building an implementation strategy for community-based rehabilitation for mental health in Colombia
Authors: Agudelo-Hernández Felipe , Rojas-Andrade Rodrigo , Giraldo Alvarez Ana Belén 
Published: May 2024

ABSTRACT

Introduction:
Scientific evidence indicates that the community-based rehabilitation (CBR) model is recommended for recovery from mental disorders. However, this approach encounters barriers and often lacks implementation strategies.

Aim:
The aim of this study was to create a strategy for the implementation of CBR for mental health in Colombia through the identification of barriers and facilitators, together with the expected outcomes, from the perspective of mental health decision-makers in Colombia.

Methods:
This study adopts a qualitative descriptive approach, using focus group data collection methods and thematic analysis to code and analyze the data.

Results:
A total of 208 individuals participated in the study, including mental health decision-makers and health care professionals. Intersectoral collaboration, contextualization, financial resources, and community commitment and autonomy were identified as barriers and facilitators. The element that was considered a priority for successful implementation was the contextualization of strategies.

Conclusions:
CBR needs to be strengthened through implementation science if these strategies are to be successfully developed and implemented in various contexts.

Spanish abstract:

http://links.lww.com/IJEBH/A210
Microbiota of healthy dogs demonstrate a significant decrease in richness and changes in specific bacterial groups in response to supplementation with resistant starch, but not psyllium or methylcellulose, in a randomized cross-over trial
Authors: Salavati Schmitz Silke , Salgado Jorge Perez-Accino , Glendinning Laura 
Published: May 2024

Graphical abstract
Microbiota of healthy dogs demonstrate significant changes in specific bacterial groups in response to supplementation with resistant starch (but not psyllium or methylcellulose) in this randomized cross-over trial.
Enterovirga aerilata sp. nov. and Knoellia koreensis sp. nov., isolated from an automobile air conditioning system
Authors: Lee Hyosun , Chaudhary Dhiraj Kumar , Kim Dong-Uk 
Published: May 2024

Two strictly aerobic and rod-shaped bacteria, labelled as DB1703T and DB2414ST, were obtained from an automobile air conditioning system. Strain DB1703T was Gram-stain-negative, while strain DB2414ST was Gram-stain-positive. Both strains were catalase-positive and oxidase-negative. Strains DB1703T and DB2414ST were able to grow at 18–42 °C. Strain DB1703T grew within a NaCl range of 0–3 % and a pH range of 6.0–8.0; while strain DB2414ST grew at 0–1 % and pH 6.5–8.5. The phylogenetic and 16S rRNA gene sequence analysis indicated that strains DB1703T and DB2414ST belonged to the genera Enterovirga and Knoellia, respectively. Strain DB1703T showed the closest phylogenetic similarity to Enterovirga rhinocerotis YIM 100770T (94.8 %), whereas strain DB2414ST was most closely related to Knoellia remsis ATCC BAA-1496T (97.7 %). The genome sizes of strains DB1703T and DB2414ST were 4 652 148 and 4 282 418 bp, respectively, with DNA G+C contents of 68.8 and 70.5 mol%, respectively. Chemotaxonomic data showed Q-10 as the sole ubiquinone in DB1703T and ML-8 (H4) in DB2414ST. The predominant cellular fatty acid in DB1703T was summed feature 8 (C18 : 1
ω7c and/or C18 : 1
ω6c), whereas iso-C16 : 0, C17 : 1
ω8c, and iso-C15 : 0 were dominant in DB2414ST. Overall, the polyphasic taxonomic comparisons showed that strains DB1703T and DB2414ST were distinct from their closest taxa and represent novel species within the genera Enterovirga and Knoellia, respectively. Accordingly, we propose the names Enterovirga aerilata sp. nov., with the type strain DB1703T (=KCTC 72724T=NBRC 114759T), and Knoellia koreensis sp. nov., with the type strain DB2414ST (=KCTC 49355T=NBRC 114620T).
Lutimonas zeaxanthinifaciens sp. nov., a zeaxanthin-producing marine bacterium isolated from coastal sediment
Authors: Jung Yoon-Hee , Kwon Yong Min , Chung Dawoon , Yu Woon-Jong , Choi Grace , Kim Tae Wan , Bae Seung Seob 
Published: May 2024

A Gram-stain-negative, yellow-pigmented, strictly aerobic, non-flagellated, motile by gliding, rod-shaped bacterium, designated strain YSD2104T, was isolated from a coastal sediment sample collected from the southeastern part of the Yellow Sea. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that strain YSD2104T was closely related to three type strains, Lutimonas vermicola IMCC1616T (97.4 %), Lutimonas saemankumensis SMK-142T (96.9 %), and Lutimonas halocynthiae RSS3-C1T (96.8 %). Strain YSD2104T has a single circular chromosome of 3.54 Mbp with a DNA G+C content of 38.3 mol%. The average nucleotide identity and digital DNA–DNA hybridization values between strain YSD2104T and the three type strains (L. vermicola IMCC1616 T, L. saemankumensis SMK-142T, and L. halocynthiae RSS3-C1T) were 74.0, 86.2 and 73.6 %, and 17.9, 30.3 and 17.8 %, respectively. Growth was observed at 20–30 °C (optimum, 30 °C), at pH 6.5–8.5 (optimum, pH 7.0), and with NaCl concentrations of 1.5–3.5 % (optimum, 2.5 %). The major carotenoid was zeaxanthin, and flexirubin-type pigment was not produced. The major respiratory quinone was menaquinone-6. The major fatty acids (>10 %) were iso-C15 : 0, iso-C15 : 1 G, iso-C17 : 0 3-OH, summed feature 3 (C16 : 1
ω6c and/or C16 : 1
ω7c), and summed feature 9 (iso-C17 : 1
ω9c and/or 10-methyl C16 : 0). The major polar lipids were phosphatidylethanolamine, one unidentified aminophospholipid, two unidentified aminolipids, and eight unidentified lipids. Conclusively, based on this polyphasic approach, we classified strain YSD2104T (=KCTC 102008T=JCM 36287T) as representing a novel species of the genus Lutimonas and proposed the name Lutimonas zeaxanthinifaciens sp. nov.
Epithelial NSD2 maintains FMOs-mediated taurine biosynthesis to prevent intestinal barrier disruption
Authors: Xu Yue , Ma Chunxiao , Wang Ziyi , Feng Wenxin , Rao Hanyu , Zhang Wei , Liu Ningyuan , Aji Rebiguli , Meng Xiangjun , Gao Wei-Qiang , Li Li 
Published: May 2024

AbstractInflammatory bowel disease (IBD) poses a significant challenge due to its intricate pathogenesis. NSD2, a histone methyltransferase responsible for dimethylating histone 3 at lysine 36, is associated with transcriptional activation. However, the precise role of NSD2 in IBD remains unexplored. In this study, we discovered a downregulation of NSD2 in both the intestinal epithelial cells (IECs) of patients and the IBD mouse model. Deficiency of NSD2 in mouse IECs aggravated epithelial barrier disruption and inflammatory response in IBD. Mechanically, NSD2 loss downregulated H3K36me2 and FMO (taurine-synthesis enzyme) mRNA in IECs, resulting in decreased taurine biosynthesis in IECs. Importantly, supplementation with taurine significantly attenuated the symptoms of NSD2 deficiency-induced IBD. These data demonstrate that NSD2 plays a pivotal role in maintaining FMOs-mediated taurine biosynthesis to prevent intestinal inflammation. Our findings also underscore the importance of NSD2-H3K36me2-mediated taurine biosynthesis in maintaining intestinal mucosal barrier homeostasis.
Design, synthesis and cellular characterization of a new class of IPMK kinase inhibitors
Authors: Zhou Yubai , Chapagain Pratima , Desmarini Desmarini , Uredi Dilipkumar , Rameh Lucia E. , Djordjevic Julianne T. , Blind Raymond D. , Wang Xiaodong 
Published: May 2024

ABSTRACTMany genetic studies have established the kinase activity of inositol phosphate multikinase (IPMK) is required for the synthesis of higher-order inositol phosphate signaling molecules, the regulation of gene expression and control of the cell cycle. These genetic studies await orthogonal validation by specific IPMK inhibitors, but no such inhibitors have been synthesized. Here, we report complete chemical synthesis, cellular characterization, structure-activity relationships and rodent pharmacokinetics of a novel series of highly potent IPMK inhibitors. The first-generation compound1(UNC7437) decreased cellular proliferation and tritiated inositol phosphate levels in metabolically labeled human U251-MG glioblastoma cells. Compound1also regulated the transcriptome of these cells, selectively regulating genes that are enriched in cancer, inflammatory and viral infection pathways. Further optimization of compound 1 eventually led to compound15(UNC9750), which showed improved potency and pharmacokinetics in rodents. Compound15specifically inhibited cellular accumulation of InsP5, a direct product of IPMK kinase activity, while having no effect on InsP6levels, revealing a novel metabolic signature detected for the first time by rapid chemical attenuation of cellular IPMK activity. These studies designed, optimized and synthesized a new series of IPMK inhibitors, which reduces glioblastoma cell growth, induces a novel InsP5metabolic signature, and reveals novel aspects inositol phosphate cellular metabolism and signaling.
Modulatory Effects of IFN-γ and IL-22 on Inflammatory Signaling and Cellular Responses in Intestinal Epithelial Cells
Authors: Johnson Asia , Denning Timothy L. 
Published: May 2024

AbstractInflammatory bowel disease (IBD) continues to affect millions worldwide, with an increasing prevalence that highlights the urgent need for deeper understanding of its underlying immune mechanisms. The cytokine interactions, especially those mediated by cells from the TH1 and TH17 lymphocyte subsets, are crucial in orchestrating the immune landscape of IBD. TH1 cells are well known for producing TNF-α and IFNγ, which have been extensively studied for their roles in conjunction with each other within the context of IBD. TH17 cells secrete IL-22 and IL-17, with existing studies primarily focusing on IL-22’s interaction with IL-17 rather than its interplay with other cytokines such as IFNγ. Our study focuses on the co-stimulatory effects of IL-22 and IFNγ using organoids derived from mouse small intestines to model epithelial interactions. We found that IFNγ interferes with the capacity of IL-22 to up-regulate antimicrobial peptides, which is essential in mucosal defense. Additionally, higher concentrations of IL-22 enhance IFNγ’s ability to stimulate TNF-α gene expression and CXCL10 protein production, indicating a dose-dependent relationship. This co-stimulation also led to an increased rate of cell death, influenced partly by TNF-α. These findings suggest that IL-22, typically seen as an anti-inflammatory agent, can assume a pro-inflammatory role when combined with IFNγ, complicating its effects on epithelial cells. This study highlights the need to consider specific cytokine interactions in developing more effective IBD treatments.
Oviposition and vertical dispersal ofAedesspecies Meigen 1818 (Diptera: Culicidae) at different heights and seasonal periods in an urban forest fragment in Manaus, Amazonas, Brazil
Authors: Ribeiro da Silva William , Arcos Adriano Nobre , da Silva Ferreira Francisco Augusto , Soares-da-Silva Joelma , Pontes Grafe Oliveira , Navarro da Silva Mário Antônio , Roque Rosemary Aparecida , Zequi João Antonio Cyrino 
Published: May 2024

ABSTRACTMosquitoes of the genusAedesstand out for their high susceptibility to several groups of arboviruses, especially those that cause dengue fever, Zika, and Chikungunya fever. However, aspects related to the vertical distribution of species in large urban centers are still poorly understood, therefore, this study aims to evaluate the dispersal and oviposition ofAedesat different height levels and seasonal periods. The study was developed in a tower with six floors located in an urban forest fragment, measuring 15.13 meters (m) high and 3.20 meters at the base. The following height ranges were considered: ground: 0 m; 1.20 m; 2.50 m; 3.60m; 4.90 m; 6m; 7.30m; 8.40m; 9.70 m; and 10.8 m. Three ovitraps were installed on each floor, separated by a distance of 1.50 m, totaling 30 for each sampling period. The ovitrap positivity index (OPI) and egg density index (EDI) were evaluated in order to monitorAedespopulations in different height ranges and also in different seasonal periods. The data demonstrated that lower heights show a greater abundance ofAedeseggs, however, this variable did not prove to be a limiting factor for mosquito colonization at the other heights evaluated. Furthermore, climatic factors, such as relative humidity have a positive influence (p<0.05) on the average number of eggs in the urban area of Manaus, especially during the dry period. These findings demonstrate that the vertical growth of urban centers can act positively tin increasing the density ofAedesand can influence the incidence of dengue and other arboviruses.
Pooled genetic screens identify breast cancer risk genes involved in evasion from T cell-mediated killing
Authors: Shi Wei , Luo Yi , Burrows Jacqueline M. , Black Debra , Civitarese Andrew , Perlaza-Jimenez Laura , Zhang Ping , Manning Murray , Tuano Natasha , Rentería Miguel E. , Xiao Christos , Tey Siok-Keen , Rosenbluh Joseph , Smith Corey , Chenevix-Trench Georgia , Beesley Jonathan 
Published: May 2024

AbstractGenome-wide association studies have identified more than 220 loci associated with breast cancer susceptibility. A major challenge is now to identify the effector genes with plausible functions in the context of breast cancer risk. We have previously performed pooled CRISPR screens to identify target genes at risk loci that drive cancer hallmarks including proliferation or modulating DNA damage response. We now extend these screens to identify genes involved in response to cytotoxic T lymphocyte (CTL) killing. We performed knockout and inhibition screens to identify genes that affect the response of the MCF7 human breast cancer cell line to CTL killing in anin vitroco-culture system. We identified 33 candidate risk genes associated with resistance or sensitisation to T cell-mediated killing. Using single gene perturbation, we showed that deletion of candidate risk genesIRF1, ATF7IP, CCDC170andCASP8induced resistance, while ablation ofCFLAR, CREBBP, andPRMT7sensitized cells to CTL killing. We used reporter assays to show that the risk-associated alleles at rs736801 and rs3769821 reduced transactivation of theIRF1andCASP8promoters, respectively. We showed that pharmacological inhibition of PRMT7 rendered breast cells sensitive to CTL killing andPRMT7levels were negatively correlated with CD8+ infiltration and patient survival in luminal A breast cancer patient cohorts. Our results demonstrate that phenotypic pooled CRISPR screens are a useful approach for high throughput functional follow-up of GWAS findings, identifying genes which alter immune responses to breast cancer which offer opportunities to enhance immunotherapy.