bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro folding of methionine‐arginine human lyspro‐proinsulin S‐sulfonate—Disulfide formation pathways and factors controlling yield
Authors: Chen Shuang , Adijanto Lawrence , Wang Nien‐Hwa Linda 
Published: April 2010

AbstractWe investigated the in vitro folding of an oxidized proinsulin (methionine‐arginine human lyspro‐proinsulin S‐sulfonate), using cysteine as a reducing agent at 5°C and high pH (10.5–11). Folding intermediates were detected and characterized by means of matrix‐assisted laser desorption ionization mass spectrometry (MALDI‐MS), reversed‐phase chromatography (RPC), size‐exclusion chromatography, and gel electrophoresis. The folding kinetics and yield depended on the protein and cysteine concentrations. RPC coupled with MALDI‐MS analyses indicated a sequential formation of intermediates with one, two, and three disulfide bonds. The MALDI‐MS analysis of Glu‐C digested, purified intermediates indicated that an intra‐A‐chain disulfide bond formed first among A6, A7, and A11. Various non‐native intra‐A (A20 with A6, A7, or A11), intra‐B (between B7 and B19), and inter‐A‐B disulfide bonds were observed in the intermediates with two disulfide bonds. The intermediates with three disulfide bonds had mainly the non‐native intra‐A and intra‐B bonds. At a cysteine‐to‐proinsulin‐SH ratio of 3.5, all intermediates with the non‐native disulfide bonds were converted to properly folded proinsulin via disulfide bond reshuffling, which was the slowest step. Aggregation via the formation of intermolecular disulfide bonds of early intermediates was the major cause of yield loss. At a higher cysteine‐to‐proinsulin‐SH ratio, some intermediates and folded MR‐KPB‐hPI were reduced to proteins with thiolate anions, which caused unfolding and even more yield loss than what resulted from aggregation of the early intermediates. Reducing protein concentration, while keeping an optimal cysteine‐to‐protein ratio, can improve folding yield significantly. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Human hematopoietic progenitor cells grow faster under rotational laminar flows
Authors: Murugappan G. , Carrillo‐Cocom L. M. , Johnson K. E. , González‐Barrón M. T. , Moreno‐Cuevas J. E. , Alvarez M. M. 
Published: April 2010

AbstractWe report significant and reproducible growth acceleration of human progenitor cells when exposed to rotational flow when compared with stationary conditions. Nonenriched CD34+ umbilical cord derived human hematopoietic progenitor cells were cultured in Petri dishes located at different radial distances with respect to the central axis of a rotating platform. Growth dynamics under 3 or 5 rpm agitation was compared against that observed under typical stationary conditions. Cells cultured at 3 or 5 rpm exhibited (a) the absence of a latency phase, (b) an increase in final cell concentrations by 54–58.5%, and (c) reduced doubling time in their exponential phase by 12–16% in comparison with stationary culture. Cells grown under rotational agitation were confirmed to remain CD34+ by PCR. These results document a significant positive effect of exposure to laminar flow fields on the growth of human hematopoietic progenitor cells. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Economic impact of total solids loading on enzymatic hydrolysis of dilute acid pretreated corn stover
Authors: Humbird David , Mohagheghi Ali , Dowe Nancy , Schell Daniel J. 
Published: April 2010

AbstractIn process integration studies of the biomass‐to‐ethanol conversion process, it is necessary to understand how cellulose conversion yields vary as a function of solids and enzyme loading and other key operating variables. The impact of solids loading on enzymatic cellulose hydrolysis of dilute acid pretreated corn stover slurry was determined using an experimental response surface design methodology. From the experimental work, an empirical correlation was obtained that expresses monomeric glucose yield from enzymatic cellulose hydrolysis as a function of solids loading, enzyme loading, and temperature. This correlation was used in a technoeconomic model to study the impact of solids loading on ethanol production economics. The empirical correlation was used to provide a more realistic assessment of process cost by accounting for changes in cellulose conversion yields at different solids and enzyme loadings as well as enzyme cost. As long as enzymatic cellulose conversion drops off at higher total solids loading (due to end‐product inhibition or other factors), there is an optimum value for the total solids loading that minimizes the ethanol production cost. The optimum total solids loading shifts to higher values as enzyme cost decreases. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Strategies for selecting recombinant CHO cell lines for cGMP manufacturing: Improving the efficiency of cell line generation
Authors: Porter Alison J. , Racher Andrew J. , Preziosi Richard , Dickson Alan J. 
Published: April 2010

AbstractTransfectants with a wide range of cellular phenotypes are obtained during the process of cell line generation. For the successful manufacture of a therapeutic protein, a means is required to identify a cell line with desirable growth and productivity characteristics from this phenotypically wide‐ranging transfectant population. This identification process is on the critical path for first‐in‐human studies. We have stringently examined a typical selection strategy used to isolate cell lines suitable for cGMP manufacturing. One‐hundred and seventy‐five transfectants were evaluated as they progressed through the different assessment stages of the selection strategy. High producing cell lines, suitable for cGMP manufacturing, were identified. However, our analyses showed that the frequency of isolation of the highest producing cell lines was low and that ranking positions were not consistent between each assessment stage, suggesting that there is potential to improve upon the strategy. Attempts to increase the frequency of isolation of the 10 highest producing cell lines, by in silico analysis of alternative selection strategies, were unsuccessful. We identified alternative strategies with similar predictive capabilities to the typical selection strategy. One alternate strategy required fewer cell lines to be progressed at the assessment stages but the stochastic nature of the models means that cell line numbers are likely to change between programs. In summary, our studies illuminate the potential for improvement to this and future selection strategies, based around use of assessments that are more informative or that reduce variance, paving the way to improved efficiency of generation of manufacturing cell lines. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Functional roles of PC‐PLC and Cdc20 in the cell cycle, proliferation, and apoptosis
Authors: Chen Zhiwei , Yu Yongfeng , Fu Da , Li Ziming , Niu Xiaoming , Liao Meilin , Lu Shun 
Published: April 2010

AbstractPhosphatidylcholine‐specific phospholipase C (PC‐PLC) is the major enzyme in the Phosphatidylcholine (PC) cycle and is involved in many long‐term cellular responses such as activation, proliferation, and differentiation events. Cell division cycle 20 homolog (Cdc20) is an essential cell‐cycle regulator required for the completion of mitosis. Our previous studies identified the interaction between PC‐PLC and Cdc20. Through the interaction, Cdc20 could mediate the degradation of PC‐PLC by Cdc20‐mediated ubiquitin proteasome pathway (UPP). In this study, we found that PC‐PLC might not be involved in cancer metastasis. Inhibition of PC‐PLC by D609 could cause cell proliferation inhibition and apoptosis inhibition in CBRH‐7919 cells. Inhibition of PC‐PLC could also influence the cell cycle by arresting the cells in G1 phase, and Cdc20 might be involved in these processes. Taken together, in this report, we provided new evidence for the functional roles of PC‐PLC and Cdc20 in the cell cycle, proliferation, and apoptosis in CBRH‐7919 cells. Copyright © 2010 John Wiley & Sons, Ltd.
ChemInform Abstract: Two Caesium Vanadium Hydrogenphosphates, with Tunnelled Structures: Cs2V2O3(PO4)(HPO4) and Cs2 [(VO)3(HPO4)4(H2O)] ·H2O.
Authors: Gautier Romain , Colin Jean‐Francois , Le Fur Eric 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Thermodynamic Modelling of the Mg—Ge—Si, Mg—Ge—Sn, Mg—Pb—Si and Mg—Pb—Sn Systems.
Authors: Jung In‐Ho , Kim Jina 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Photogeneration of Nitrosyl Linkage Isomers in Octahedrally Coordinated Platinum Complexes in the Red Spectral Range.
Authors: Schaniel Dominik , Woike Theo , Behrnd Norwid‐R. , Hauser Juerg , Kraemer Karl W. , Todorova Teodora , Delley Bernard 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Diastereomeric P*‐Mono‐ and P*, P*‐Bidentate Diamidophosphite Ligands Based on 1,4:3,6‐Dianhydro‐D‐mannitol in Asymmetric Metallocomplex Catalysis.
Authors: Lyubimov S. E. , et al. et al. 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Synthetic Protocol of trans‐Substituted Cyclopentenes via the Ring‐Opening Rearrangement of MCP Alkenyl Derivatives.
Authors: Tang Xiang‐Ying , Shi Min 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Reductive Dehalogenation of Aryl Bromides and Chlorides and Their Use as Aryl Blocking Groups.
Authors: Ramanathan Ahalya , Jimenez Leslie S. 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Rapid Reduction of Heteroaromatic Nitro Groups Using Catalytic Transfer Hydrogenation with Microwave Heating.
Authors: Quinn John F. , Bryant Cole E. , Golden Kathryn C. , Gregg Brian T. 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: CeCl3·7H2O as an Efficient Catalyst for One‐Pot Synthesis of β‐Amino Ketones by Three‐Component Mannich Reaction.
Authors: Dai Yan , Li Bin Dong , Quan Heng Dao , Lue Chun Xu 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Enzymatic Kinetic Resolution of Racemic Cyanohydrins via Enantioselective Acylation.
Authors: Xu Qing , Xie Yongli , Geng Xiaohong , Chen Peiran 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Ligand‐Enabled Reactivity and Selectivity in a Synthetically Versatile Aryl C—H Olefination.
Authors: Wang Dong‐Hui , Engle Keary M. , Shi Bing‐Feng , Yu Jin‐Quan 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Anodic Oxidation and Organocatalysis: Direct Regio‐ and Stereoselective Access to meta‐Substituted Anilines by α‐Arylation of Aldehydes.
Authors: Jensen Kim L. , Franke Patrick T. , Nielsen Lasse T. , Daasbjerg Kim , Joergensen Karl Anker 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Stereodefined 3‐Alkylideneoxindoles by Palladium‐Catalyzed Reactions of 2‐(Alkynyl)aryl Isocyanates with Thiols and Alcohols.
Authors: Miura Tomoya , Toyoshima Takeharu , Ito Yoshiteru , Murakami Masahiro 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Practical Method for the Synthesis of 2,3‐Disubstituted Indole Derivatives Promoted by β‐(Benzotriazol‐1‐yl)allylic O‐Stannyl Ketyl Radicals.
Authors: Kim Taehoon , Kim Kyongtae 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Unprecedented Rearrangement of 2‐(2‐Aminoethyl)‐1‐aryl‐3,4‐dihydropyrazino[1,2‐b]indazole‐2‐ium 6‐Oxides to 2,3‐Dihydro‐1H‐imidazo[1,2‐b]indazoles.
Authors: Koci Jan , Oliver Allen G. , Krchnak Viktor 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Microwave‐Controlled Preparation of Alkenyl‐(1H)‐1,2,4‐triazoles: First Heck Reaction on a (1H)‐1,2,4‐Triazole Moiety.
Authors: Diaz‐Ortiz Angel , Prieto Pilar , de Cozar Abel , Cebrian Cristina , Moreno Andres , de la Hoz Antonio 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Facile Synthesis of Substituted 5‐Amino‐ and 3‐Amino‐1,2,4‐thiadiazoles from a Common Precursor.
Authors: Wehn Paul M. , Harrington Paul E. , Eksterowicz John E. 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Novel aza‐Prins‐Friedel—Crafts Reaction for the Synthesis of 4‐Arylpiperidines.
Authors: Yadav J. S. , Reddy B. V. Subba , Ramesh K. , Kumar G. G. K. S. Narayana , Gree Rene 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Stereoselective Synthesis of 2‐Substituted 6‐[1‐(2,6‐Difluorophenyl)ethyl]‐5‐methylpyrimidin‐4(3H)‐ones.
Authors: Mai A. , Artico M. , Rotili D. , Novakov I. A. , Orlinson B. S. , Navrotskii M. B. , Eremiichuk A. S. , Gordeeva E. A. 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Substituted 3,4‐Dihydropyrimidin‐2(1H)‐ones and Pyrimidin‐2(1H)‐ones by the Biginelli Reaction with 3,5‐Di‐tert‐butyl‐4‐hydroxybenzaldehyde.
Authors: Sedova V. F. , Krivopalov V. P. , Shkurko O. P. 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of N‐Substituted Pyrrolo[1,2‐a][1,4]benzodiazepines.
Authors: Stroganova T. A. , Malikov I. V. 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Novel 5‐Phenylselenenyl Pyrimidine Analogues
Authors: Basha N. Jeelan , Reddy C. H. Upendar , Goudgaon N. M. 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Unusual Products of the Reactions of Verbenone and Verbenol with N‐Bromosuccinimide in the Presence of Water.
Authors: Ardashov O. V. , Il'ina I. V. , Korchagina D. V. , Volcho K. P. , Salakhutdinov N. F. 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Two Novel Neoclerodane Diterpenoids from Scutellaria barbata.
Authors: Lee Hanna , Kim YuJin , Choi Inho , Min Byung Sun , Shim Sang Hee 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: C—C Bond Formation on 5‐Position of Uridine Ring by Morita—Baylis—Hillman Type Reaction.
Authors: Monguchi Yasunari , Yasunaga Kanoko , Tsunoda Takashi , Ando Takayuki , Maegawa Tomohiro , Hirota Kosaku , Sajiki Hironao 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Total Synthesis of a Diarylheptanoid, Rhoiptelol B.
Authors: Reddy Chada Raji , Rao Nagavaram Narsimha , Srikanth Boinapally 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Neolignans from Piper kadsura and Their Antineuroinflammatory Activity.
Authors: Kim Ki Hyun , Choi Jung Wook , Ha Sang Keun , Kim Sun Yeou , Lee Kang Ro 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: 2,4‐Diaryl‐4,6,7,8‐tetrahydroquinazolin‐5(1H)‐one Derivatives as anti‐HBV Agents Targeting at Capsid Assembly.
Authors: Zhu Xuejun , Zhao Guoming , Zhou Xiaoping , Xu Xiaoqian , Xia Guangqiang , Zheng Zhibing , Wang Lili , Yang Xiaohong , Li Song 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Coumarins as Novel 17β‐Hydroxysteroid Dehydrogenase Type 3 Inhibitors for Potential Treatment of Prostate Cancer.
Authors: Harada Koichiro , Kubo Hideki , Tomigahara Yoshitaka , Nishioka Kazuhiko , Takahashi Junya , Momose Mio , Inoue Shinichi , Kojima Atsuyuki 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Unusual Framework Stabilization of Cu(II) and Cu(I) Ions in a Novel Copper‐Substituted Aluminophosphate with AEN Topology Prepared by One‐Pot Synthesis
Authors: Gabelica Z. , Fernandes A. , Ribeiro M. F. , Lourenco J. P. , Valange S. , Louati A. , Murphy D. M. 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.