bluuebunny/crossref_metadata_2025_split huggingface dataset:
The SK3/KCa2.3 potassium channel is a new cellular target for edelfosine
Authors: Potier M , Chantome A , Joulin V , Girault A , Roger S , Besson P , Jourdan M‐L , LeGuennec J‐Y , Bougnoux P , Vandier C 
Published: October 2010

BACKGROUND AND PURPOSE The 1‐O‐octadecyl‐2‐O‐methyl‐sn‐glycero‐3‐phosphocholine (edelfosine) is an ether‐linked phospholipid with promising anti‐cancer properties but some side effects that preclude its full clinical therapeutic exploitation. We hypothesized that this lipid could interact with plasma membrane ion channels and modulate their function.EXPERIMENTAL APPROACH Using cell migration‐proliferation assays, patch clamp, spectrofluorimetry and 125I‐Apamin binding experiments, we studied the effects of edelfosine on the migration of breast cancer MDA‐MB‐435s cells, mediated by the small conductance Ca2+‐activated K+ channel, SK3/KCa2.3.KEY RESULTS Edelfosine (1 µM) caused plasma membrane depolarization by substantially inhibiting activity of SK3/KCa2.3 channels, which we had previously demonstrated to play an important role in cancer cell migration. Edelfosine did not inhibit 125I‐Apamin binding to this SKCa channel; rather, it reduced the calcium sensitivity of SK3/KCa2.3 channel and dramatically decreased intracellular Ca2+ concentration, probably by insertion in the plasma membrane, as suggested by proteinase K experiments. Edelfosine reduced cell migration to the same extent as known SKCa channel blockers. In contrast, K+ channel openers prevented edelfosine‐induced anti‐migratory effects. SK3 protein knockdown decreased cell migration and totally abolished the effect of edelfosine on MDA‐MB‐435s cell migration. In contrast, transient expression of SK3/KCa2.3 protein in a SK3/KCa2.3‐deficient cell line increased cell migration and made these cells responsive to edelfosine.CONCLUSIONS AND IMPLICATIONS Our data clearly establish edelfosine as an inhibitor of cancer cell migration by acting on SK3/KCa2.3 channels and provide insights into the future development of a new class of migration‐targeted, anti‐cancer agents.
Off‐farm work, technical efficiency, and rice production risk in Taiwan
Authors: Chang Hung‐Hao , Wen Fang‐I 
Published: October 2010

AbstractThis article investigates the differences in yield production, production efficiency, and yield risk for farmers both with and without off‐farm work. Using a nationwide survey of rice farmers in Taiwan, we estimate two stochastic production frontier models that accommodate technical inefficiency and production risk simultaneously for farmers both with and without off‐farm work. The stochastic dominance criterion is then applied to compare the differences in the distributions of the estimated technical efficiency and yield risk between groups. The empirical results indicate that these two groups of farmers use resources in different ways, and off‐farm work is not necessarily associated with lower technical efficiency. For farmers in the lower percentiles of the efficiency distribution, those with off‐farm work are more efficient than their counterparts without off‐farm work. In addition, farmers with off‐farm work face higher production risk and this result is robust for the entire distribution.
Repeat cerclage prolongs pregnancy in women with prolapsed membranes
Authors: SONG JI‐EUN , LEE KEUN‐YOUNG , JUN HYUN‐AH 
Published: October 2010

ABSTRACTWe evaluated repeat cerclage in women with prolapsed membranes. Twenty‐two women with bulging membranes after primary cerclage were offered repeat cerclage; 11 chose a repeat cerclage and 11 chose bed rest. The median gestational age at delivery, birthweight and survival rates were significantly higher in the repeat cerclage group compared to the bed rest group (mean 26.8 weeks vs. 21.7 weeks, p= 0.04, mean birthweight 1180 g vs. 491 g, p= 0.01 odds ratio for survival 22.0, 95% CI, 2.1–236). Early repeat cerclage under antibiotic cover may be beneficial in women with bulging membranes after a prior failing cerclage attempt.
Surgical‐site infections and postoperative complications: agreement between the Danish Gynecological Cancer Database and a randomized clinical trial
Authors: ANTONSEN SOFIE L. , MEYHOFF CHRISTIAN S. , LUNDVALL LENE , HØGDALL CLAUS 
Published: October 2010

ABSTRACT Objective. Surgical‐site infections are serious complications and thorough follow‐up is important for accurate surveillance. We aimed to compare the frequency of complications recorded in a clinical quality database with those noted in a randomized clinical trial with follow‐up visits. Design. Evaluation study. Setting. Danish Gynecological Cancer Database (DGCD) and the Danish multicenter trial on perioperative oxygen and surgical‐site infections (PROXI). Sample. Paired data from 222 patients who participated in the PROXI trial taking place at Copenhagen University Hospital, Rigshospitalet between November 2006 and October 2008 and data from the DGCD. Methods. Outcomes within 30 days from the trial and the database were compared and levels of agreements were calculated with kappa‐statistics. Main Outcome Measures. Primary outcome was surgical‐site infection. Other outcomes included re‐operation, urinary tract infection, pneumonia and sepsis. Results. Surgical‐site infection was found in 21 of 222 patients (9.5%) in the PROXI trial versus 6 of 222 patients (2.7%) in the DGCD (p < 0.01, kappa 0.42). Twelve of 15 superficial and three of six deep or organ‐space surgical‐site infections were registered in the PROXI trial, but not in the DGCD. Agreements between secondary outcomes were very varying (kappa‐value 0.77 for re‐operation, 0.37 for urinary tract infections, 0.19 for sepsis and 0.18 for pneumonia). Conclusions. The randomized trial reported significantly more surgical‐site infections than the clinical database. The DGCD reported only 50% of the deep and organ‐space infections, and hence, the low‐overall agreement indicates a need for more careful database registration.
Eclampsia is more prevalent during the winter season in Sweden
Authors: RYLANDER ÅSA , LINDQVIST PELLE G. 
Published: October 2010

ABSTRACTHypertensive pregnancy conditions have shown seasonal variations and have been related to low levels of vitamin D. In Sweden, the hours of sunlight per day are few in winter. We hypothesized that eclampsia would be more common in the winter season. We performed a population‐based cross‐sectional study of all singleton deliveries in Sweden during a 5‐year period (n= 482,759 women). All parturients with eclampsia were included as cases and those without formed control group. The incidence of eclampsia was nearly doubled during winter season (odds ratio 1.9, 95% confidence interval 1.4–2.6) as compared to other seasons. The incidence of eclampsia during the period studied (1990–1994) was four in 10,000 pregnancies. We found that eclampsia was twice as common in winter and we introduce the alternative hypothesis that lack of sunlight exposure increases women's susceptibility to eclampsia, possibly due to vitamin D insufficiency.
Factors related to elevated vaginal pH in the first trimester of pregnancy
Authors: ZODZIKA JANA , REZEBERGA DACE , JERMAKOVA IRINA , VASINA OLGA , VEDMEDOVSKA NATALIJA , DONDERS GILBERT 
Published: October 2010

ABSTRACT Objective. To assess different bacterial and epidemiological factors associations with increased vaginal pH in the pregnant women population during the first trimester. Design. A cross‐sectional, observational study. Setting. Three outpatient clinics in Riga. Population. From July 2009 until January 2010, 139 unselected consecutive pregnant women at the first prenatal visit. Methods. Pregnant women were submitted to an interview, vaginal examination and vaginal specimen collection for pH measurement and native microscopy. Main outcome measures. Vaginal pH ≥4.5 was considered as elevated. Abnormal bacterial microflora was classified according to Donders. Results. Elevated vaginal pH was significantly associated with bacterial vaginosis (p < 0.001), aerobic vaginitis (p < 0.001) and mixed aerobic vaginitis and bacterial vaginosis flora (p < 0.001) and presence of sperm cells in the smears (p= 0.024). Most cases with sperm were associated with abnormal vaginal flora. Normal lactobacillary morphotypes were more often found in the pH ≤4.4 group (p < 0.001), while leptosomic and short types were found more frequently with increased pH. Conclusions. Elevated vaginal pH is associated with different types of abnormal vaginal flora and the presence of sperm cells.
The severity of clinical manifestations in preeclampsia correlates with the amount of placental infarction
Authors: VINNARS MARIE‐THERESE , NASIELL JOSEFINE , GHAZI SAM , WESTGREN MAGNUS , PAPADOGIANNAKIS NIKOS 
Published: October 2010

ABSTRACT Objective. To correlate placental histopathology, in particular ischemic changes, with the clinical severity of preeclampsia. Design. A blinded retrospective study. Setting. One Swedish hospital. Sample. One hundred and fifty‐seven women with severe (n= 116) or mild (n= 41) preeclampsia and 157 normotensive women matched according to gestational‐age. Methods: One senior pathologist, blinded to clinical data and group, examined all histological slides. In the statistical analyses, adjustment for gestational week was done when appropriate. Main outcome measures. Placental histopathological findings. Results: Amount of infarction increased with the severity of preeclampsia (p < 0.001). Infarction involving ≥5% of the placental tissue was seen in 39.7% of severe preeclampsia, 17.1% of mild preeclampsia and 5.1% of non‐preeclampsia. When comparing placentas in severe preeclampsia, mild preeclampsia and non‐preeclampsia, there was an increase in the presence of any infarction (80.2%, 61.0%, vs. 20.4%). Also, there was a difference in the presence of decidual arteriopathy (35.3%, 22.0%, vs. 3.8%) and accelerated villous maturation (71.6%, 53.3%, vs. 12.6%). We found no difference in intervillous thrombosis, abruption placenta or placental weight in relation to gestational week. Conclusions. In pregnancies with mild or severe preeclampsia, a large proportion of the placentas had histological signs of pathology, in particular signs of ischemia. The pathology was similar, but more pronounced in severe compared to mild preeclampsia, suggesting mild and severe preeclampsia to have similar underlying etiology.
Prevalence of anemia and micronutrient deficiencies in early pregnancy in rural Bangladesh, the MINIMat trial
Authors: LINDSTRÖM EMMA , HOSSAIN MOHAMMAD B. , LÖNNERDAL BO , RAQIB RUBHANA , EL ARIFEEN SHAMS , EKSTRÖM EVA‐CHARLOTTE 
Published: October 2010

ABSTRACT Objective. To describe the prevalence of anemia and micronutrient deficiencies as well as their determinants in early pregnancy. Design. Baseline data from a population‐based randomized intervention trial. Setting. The study was conducted in Matlab, a sub‐district in rural Bangladesh from 1 January to 31 December 2002. Population. Pregnant women (n= 740) were enrolled in approximately week 14 in pregnancy. Methods. Data were collected using questionnaires, physical examinations and laboratory analyses of blood samples for concentrations of hemoglobin, ferritin, zinc, folate and vitamin B‐12. Main Outcome Measures. Covariates associated with anemia and micronutrient deficiencies in bivariate analyses were evaluated in multivariate logistic regression models adjusting for potential confounders. Results. Anemia was present in 28% of the women, 55% were zinc deficient, 46% were vitamin B‐12 deficient and 18% were folate deficient. Anemia was not associated with iron deficiency but rather with vitamin B‐12 deficiency. Infestation with Ascaris was highly prevalent (67%) and associated with both folate and vitamin B‐12 deficiency. Anemia and micronutrient deficiencies all varied significantly with season. Conclusions. The high prevalences of zinc and vitamin B‐12 deficiencies in early pregnancy are a concern, as it could lead to adverse pregnancy outcomes and increased health risks for both mother and child. The prevalence of iron deficiency was low, but as this was during early pregnancy, the women might develop iron deficiency and consequently iron deficiency anemia as the pregnancy progresses.
A region within the C‐terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross‐linkers and mass spectrometry
Authors: Redeker Virginie , Bonnefoy Jonathan , Le Caer Jean‐Pierre , Pemberton Samantha , Laprévote Olivier , Melki Ronald 
Published: October 2010

The propagation of yeast prion phenotypes is highly dependent on molecular chaperones. We previously demonstrated that the molecular chaperone Ssa1p sequesters Ure2p in high molecular weight, assembly incompetent oligomeric species. We also determined the affinity of Ssa1p for Ure2p, and its globular domain. To map the Ure2p–Ssa1p interface, we have used chemical cross‐linkers and MS. We demonstrate that Ure2p and Ssa1p form a 1 : 1 complex. An analytical strategy combining in‐gel digestion of cross‐linked protein complexes, and both MS and MS/MS analysis of proteolytic peptides, allowed us to identify a number of peptides that were modified because they are exposed to the solvent. A difference in the exposure to the solvent of a single lysine residue, lysine 339 of Ure2p, was detected upon Ure2p–Ssa1p complex formation. These observations strongly suggest that lysine 339 and its flanking amino acid stretches are involved in the interaction between Ure2p and Ssa1p. They also reveal that the Ure2p amino‐acid stretch spanning residues 327–339 plays a central role in the assembly into fibrils.Structured digital abstract
MINT‐8044534: Ure2p (uniprotkb:Q8NJQ9) and Ure2p (uniprotkb:Q8NJQ9) bind (MI:0407) by cross‐linking study (MI:0030)
MINT‐8044522: Ssa1p (uniprotkb:C8Z3H3) and Ssa1p (uniprotkb:C8Z3H3) bind (MI:0407) by cross‐linking study (MI:0030)
MINT‐8043971, MINT‐8043985, MINT‐8044494, MINT‐8044548: Ure2p (uniprotkb:Q8NJQ9) and Ssa1p (uniprotkb:C8Z3H3) bind (MI:0407) by cross‐linking study (MI:0030)
Predicting the incidence of planet and debris discs as a function of stellar mass
Authors: Greaves J. S. 
Published: October 2010

Abstract
The mass of solids in a young circumstellar disc may be the key factor in its efficiency in building planetesimals and planetary cores, and dust observed around young T Tauri and Herbig Ae stars can be used as a proxy for this initial solid content. The dust-mass distributions are taken from recent millimetre-wavelength data and fitted using survival analysis to take into account upper limits, and threshold disc masses for building planets and belts of comets are estimated. Amongst A stars, 20 per cent gas giant and 55 per cent debris disc systems are predicted, in good agreement with observations. For M stars, the predicted and observed planet frequencies agree at ∼2–3 per cent, and this low incidence is explained by a lack of massive discs. However, debris is predicted around ≈14 per cent of M stars, while only ∼2 per cent such systems have so far been found. This suggests that deeper searches such as with Herschel and SCUBA-2 may find a cold disc population previously missed around these low-luminosity stars. Also, an estimate of the efficiency of building millimetre-detected dust into planetary cores suggests that about one-third of M stars could host an Earth-mass planet – but as the dust is spread over large disc areas, such planets may orbit far from the star.
Women's decision making during the menopausal transition
Authors: Theroux Rosemary 
Published: October 2010

Abstract Purpose: To describe women's experiences during decision making about hormonal and nonhormonal therapies during the menopausal transition. Data Sources: Transcripts from 21 semi‐structured audio taped interviews with seven peri‐ and postmenopausal women who had a recently visited a nurse practitioner (NP) and were making a decision about menopausal management. Conclusions: Decision making was a nonlinear process in which women considered available options, weighed benefits and risks and likely outcomes. Reevaluation of the decision was ongoing. Both internal and external conditions influenced their decisions. Media reports of findings from the Women's Health Initiative study may have influenced some women's perceptions of the risk of using hormones for symptom relief. Implications for Practice: Women described caring and empowering consultations with the NPs. They appreciated provision of information, adequate time spent at the visit, and decision support. NPs have a critical role to play in providing women with current research findings about hormone therapy and alternatives for symptom relief, and assisting women with understanding risks and benefits of each possible choice. Both individual and group approaches for decision support should be available to women. The approach of a collaborative partnership in decision making is a model that is congruent with nursing practice.
Emancipatory Actions Displayed by Multi‐Ethnic Women: “Regaining Control of My Health Care”
Authors: Alexander Ivy M. 
Published: October 2010

Abstract Purpose: Despite the recognized importance of patient involvement in primary care interactions, little information describing women's needs and expectations for these interactions is available. This participatory action study was based in Critical Action Theory and designed to describe any emancipatory interests that surfaced when eight ethnically diverse women examined their interactions with primary care nurse practitioners (PCNPs) over the course of five successive focus group meetings. Data Sources: Focus group meeting transcripts, field notes, interaction notations, seating maps, and first impression summaries. Conclusions: Participants wanted to learn how to “stand up” for themselves in primary care interactions. They believed this could be accomplished by developing a positive sense of self‐esteem. Ultimately, they identified the right way to “talk back” to clinicians and created a method for regaining control of their own health care and maintaining equality in interactions with primary care clinicians. Implications for Practice: Nurse practitioners working in the primary setting are especially well situated to support self‐management and foster patient participation by women as they live with chronic disease, engage in health promotion activities, and deal with common symptomatic problems for themselves and their families.
Fiber's impact on high‐sensitivity C‐reactive protein levels in cardiovascular disease
Authors: Butcher Jennifer L. , Beckstrand Renea L. 
Published: October 2010

Abstract Purpose: To determine if increased dietary or supplemental intake of fiber slows or prevents inflammation as evidenced by high‐sensitivity C‐reactive protein (hs‐CRP) values. Data sources: CINAHL, Medline, Health Source, Nursing/Academic Edition, and the Cochrane Library. Conclusions: Evidence from this review suggested significant associations between fiber consumption and decreased risk for cardiovascular (CV) disease as evidenced by a decrease in hs‐CRP levels. Six of the seven articles reviewed showed statistically significant decreases in hs‐CRP levels as dietary fiber was increased. Implications for practice: Fiber in the diet may play a strong role in CV health as evidenced by six clinical trials completed using amount of fiber intake in relation to inflammation, particularly hs‐CRP levels. Patients need to be educated to adhere to a high fiber diet, either by dietary or supplemental means, using the recommended 25–30 g of fiber per day.
Oral 5‐aminosalicylic acid therapy for mild‐to‐moderate ulcerative colitis
Authors: Olmstead Jill 
Published: October 2010

Abstract Purpose: 5‐aminosalicylic acid (5‐ASA) is the recommended first‐line treatment for patients with ulcerative colitis (UC). These patients frequently fail to receive the full benefit from this treatment, often as a result of nonadherence to complex dosing regimens. The purpose of this review is to update nurse practitioners (NPs) on the available 5‐ASA formulations for patients with UC and to explore the role of NPs in the effective use of these drugs in disease management. Data sources: Pubmed and recent conference abstracts were searched for studies that examined either 5‐ASA therapy in UC or the role of NPs in their treatment. Conclusions: Patients frequently fail to adhere to their 5‐ASA treatment regimens, perhaps owing to a lack of understanding about their disease, or a lack of awareness of their medical management options. The unique relationship between patients and NPs allow barriers to treatment success to be identified and overcome. Implications for practice: For patients with UC, NPs are often a primary point of contact, and are therefore ideally placed to take steps to positively influence and change patient behavior.
Temperature dependence of haemoglobin–oxygen affinity in heterothermic vertebrates: mechanisms and biological significance
Authors: Weber R. E. , Campbell K. L. 
Published: October 2010

AbstractAs demonstrated by August Krogh et al. a century ago, the oxygen‐binding reaction of vertebrate haemoglobin is cooperative (described by sigmoid O2 equilibrium curves) and modulated by CO2 and protons (lowered pH) that – in conjunction with later discovered allosteric effectors (chloride, lactate and organic phosphate anions) – enhance O2 unloading from blood in relatively acidic and oxygen‐poor tissues. Based on the exothermic nature of the oxygenation of the haem groups, haemoglobin–O2 affinity also decreases with rising temperature. This thermal sensitivity favours oxygen unloading in warm working muscles, but may become detrimental in regionally heterothermic animals, for example in cold‐tolerant birds and mammals and warm‐bodied fish, where it may perturb the balance between O2 unloading and O2 requirement in organs with substantially different temperatures than at the respiratory organs and thus commonly is reduced or obliterated. Given that the oxygenation of haemoglobin is linked with the endothermic release of allosteric effectors, increased effector interaction is an effective strategy that is widely exploited to achieve adaptive reductions in the temperature dependence of blood‐O2 affinity. The molecular mechanisms implicated in heterothermic vertebrates from different taxonomic groups reveal remarkable variability, both as regards the effectors implicated (protons in tunas, organic phosphates in sharks and billfish, chloride ions in ruminants and chloride and phosphate anions in the extinct woolly mammoth, etc.) and binding sites for the same effectors, indicating multiple evolutionary origins, but convergent physiological functionality (reductions in temperature dependence of O2‐binding affinity that safeguard tissue O2 supply).