bluuebunny/crossref_metadata_2025_split huggingface dataset:
Visual impairment and blindness in rural central India: the Central India Eye and Medical Study
Authors: Nangia Vinay , Jonas Jost B. , Gupta Rajesh , Khare Anshu , Sinha Ajit 
Published: June 2012

Abstract.Purpose:  The aim of the study was to investigate prevalence of visual impairment in rural central India.Methods:  The population‐based Central India Eye and Medical Study included 4711 subjects with an age of 30+ years. Presenting visual acuity (PRVA) and best‐corrected visual acuity (BCVA) were recorded. Visual impairment and blindness were defined using the World Health Organization (WHO) standard and United States (US) standard.Results:  On the basis of PRVA and using WHO and US standards, 1049 [22%; 95% confidence interval (CI): 21.1, 23.5] subjects and 1290 (27%; 95% CI: 26.1, 28.7) subjects, respectively, were visually impaired, and 35 (0.7%; 95% CI: 0.5, 1.0) subjects and 116 (2.5%; 95% CI: 2.0, 2.9) subjects, respectively, were blind. The corresponding age‐standardized prevalence figures were 17%, 21%, 0.5% and 2%, respectively. Using best‐correcting glasses could eliminate PRVA‐visual impairment/blindness in 729 subjects (67% of all subjects with visual impairment/blindness). On the basis of BCVA and using WHO and US standards, 333 (7%; 95% CI: 6.3, 7.8) subjects and 473 (10%; 95% CI: 9.2, 10.9) subjects, respectively, had visual impairment, and 22 (0.5%; 95% CI: 0.3, 0.7) and 31 (0.7%; 95% CI: 0.4, 0.9) subjects, respectively, were blind. Corresponding age‐standardized prevalence figures were 5%, 8%, 0.4% and 0.5%, respectively. Causes for BCVA‐visual impairment/blindness were cataract (75%), postoperative posterior capsular opacification (4%), surgical complications (2%), corneal opacifications (2%), age‐related macular degeneration (2%), other macular diseases (1%), and glaucoma (1%).Conclusions:  Age‐standardized prevalence of PRVA‐visual impairment/blindness (WHO definition) in the adult population of rural central India was 17%. Most frequent cause was undercorrected refractive error. Supply of correct glasses is the most efficient way to improve vision in the rural central India.
Merging the Profession: A Historical Perspective on Accounting Association Mergers in Canada
Authors: Richardson Alan J. , Kilfoyle Eksa 
Published: June 2012

AbstractThe history of creation of new professional accounting associations, mergers and proposed mergers among professional associations in Canada is reviewed through the lens of institutional theory to identify the conditions associated with major changes in the structure of the profession. Five waves of merger activity are identified between 1880 and 2010. These waves of mergers: (1) created isomorphism between the structure of accounting associations and the political structure of Canada, (2) differentiated associations to reflect the division of labour among accountants (primarily the separation of public and management accounting), (3) differentiated associations to reflect different educational paths into accounting (broadly conceived), and (4) consolidated the profession to facilitate the regulation of public accounting by the state. Contemporary issues affecting the structure of the profession are also identified including the globalization of accounting credentials. The historical waves of merger activity are argued to be an enactment of state and market institutional logics. These factors are compared with justifications/rationales and proposals for the merger of the accounting profession under the Chartered Professional Accountant, CPA, designation. The merger proposal would create a hybrid organization that retains within it historical institutional patterns. Issues concerning the stability of hybrid organizations in the literature are identified.
popdemo: an R package for population demography using projection matrix analysis
Authors: Stott Iain , Hodgson David J. , Townley Stuart 
Published: June 2012

Summary1. Effective population management requires accurate predictions of future population dynamics and how they may be manipulated to achieve management goals.2. The R package popdemo provides software tools for novel analytical methods that aim to enhance the predictive power of basic population projection matrix models. These include indices of transient population dynamics and transfer function analyses.3. We use a case study to demonstrate the use and importance of these methods for population management and briefly discuss their potential application outside population ecology.
Drug–drug interactions in the medical intensive care unit: an assessment of frequency, severity and the medications involved
Authors: Smithburger Pamela L , Kane-Gill Sandra L , Seybert Amy L 
Published: June 2012

Abstract

Objectives
Mortality and morbidity are increased in patients experiencing drug–drug interactions (DDIs). Critically ill patients are at an increased risk of adverse events from DDIs due to the large number of medications that they take and their changes in organ function. Currently, there is a lack of literature describing DDIs in the intensive care unit (ICU). The purpose of this study is to evaluate frequency, severity and drug combinations involved in DDIs occurring in a medical ICU (MICU).

Methods
This was a 4-week, prospective, observational study that was conducted in the MICU of an academic medical centre. Lexi-Interact and Micromedex interaction databases were utilized daily to screen patients’ medication profiles for DDIs, and severity was assessed using each database's severity rating scale.

Key findings
Of 240 patient medication profiles evaluated, 457 DDIs were identified. The rate of DDIs was 190.4 DDIs/100 patient days with 297 of these interactions being unique drug pairs. About 25% (114/457) were considered major DDIs. The most commonly involved medications were antihypertensive medications (106/457) and anticoagulants/antiplatelet agents (80/457).

Conclusions
DDIs occur frequently in the MICU. Severity and drug combinations related to DDIs in the MICU differ from DDIs published in other ICU settings. When developing a DDI alerting system, patient characteristics and location should be considered.
Complete Genome Sequence of Phototrophic Betaproteobacterium Rubrivivax gelatinosus IL144
Authors: Nagashima Sakiko , Kamimura Akiko , Shimizu Takayuki , Nakamura-Isaki Sanae , Aono Eiji , Sakamoto Koji , Ichikawa Natsuko , Nakazawa Hidekazu , Sekine Mitsuo , Yamazaki Shuji , Fujita Nobuyuki , Shimada Keizo , Hanada Satoshi , Nagashima Kenji V. P. 
Published: June 2012

ABSTRACT

Rubrivivax gelatinosus
is a facultative photoheterotrophic betaproteobacterium living in freshwater ponds, sewage ditches, activated sludge, and food processing wastewater. There have not been many studies on photosynthetic betaproteobacteria. Here we announce the complete genome sequence of the best-studied phototrophic betaproteobacterium,
R. gelatinosus
IL-144 (NBRC 100245).
Draft Genome Sequence of the Nitrophenol-Degrading Actinomycete Rhodococcus imtechensis RKJ300
Authors: Vikram Surendra , Kumar Shailesh , Subramanian Srikrishna , Raghava Gajendra Pal Singh 
Published: June 2012

ABSTRACT

We report the 8.231-Mb genome sequence of
Rhodococcus imtechensis
RKJ300, isolated from pesticide-contaminated soil in Punjab, India. The genome sequence of the strain RKJ300 will be helpful in exploring the molecular pathways involved in the degradation of nitrophenols.
The safety of anticoagulation in patients with intracranial aneurysms
Authors: Tarlov Nicholas , Norbash Alexander M , Nguyen Thanh N 
Published: June 2012

Background and purposeLittle is known about the safety of long-term anticoagulation in patients with unruptured saccular intracranial aneurysms, although case reports of anticoagulation in patients with fusiform and saccular aneurysms have been published. The goal of this study was to estimate the rate of subarachnoid hemorrhage (SAH) due to aneurysm rupture in patients with intracranial saccular aneurysms receiving therapeutic doses of anticoagulation.MethodsThis was a retrospective case series of patients with intradural aneurysms receiving therapeutic doses of anticoagulation (defined as a dose that achieves a target INR >2.0 or the equivalent dose of another anticoagulant). The primary outcome was SAH due to aneurysm rupture while receiving anticoagulation treatment. The rate of SAH due to aneurysm rupture was calculated among patients with aneurysms documented on imaging.Results42 patients with 48 intradural aneurysms on anticoagulation were identified. Their mean age was 67 years (range 40–83) and 28 (67%) were women. The mean aneurysm size was 5.1±3.6 mm and 40 (83%) aneurysms were in the anterior circulation. The mean±SD follow-up was 495±749 days per patient. Twelve patients had their aneurysms coiled, clipped or surgically wrapped before the period of anticoagulation. Three patients had clipping or coiling of their aneurysms during the period of anticoagulation. Six patients had a history of aneurysm rupture prior to anticoagulation, of which two were treated by coiling and four by clipping. Among the 48 aneurysms, 32 were never coiled or clipped prior to anticoagulation. No anticoagulated patients with known aneurysms developed SAH during 57 patient-years of follow-up. The patients with untreated aneurysms had 31 patient-years of follow-up and those who underwent clipping, coiling or surgical wrapping of their aneurysms had 26 patient-years of follow-up while receiving anticoagulation (for a combined total of 57 years of follow up).ConclusionsThe risk of aneurysm rupture is not increased in patients receiving systemic anticoagulation. However, these results should be interpreted with caution given the small sample size in this study and the need for a prospective study to confirm these findings.
Levels of mortality in hens by end of lay on farm and in transit to slaughter in Great Britain
Authors: Weeks C. A. , Brown S. N. , Richards G. J. , Wilkins L. J. , Knowles T. G. 
Published: June 2012

The average mortality for end of lay hens dead on arrival (DOA) was 0.27 per cent (median 0.15 per cent) in a survey of 13.3 million hens transported during 2009. A statistical model of the data indicated main risk factors for DOA to be slaughter plant, distance travelled and external air temperature, with longer journeys and low external air temperatures increasing the risk. Other highly significant risk factors (P < 0.001) related to the condition of the birds on farm, where an increased risk of DOA was positively associated with poor feather cover, lower body weight, cumulative mortality of the flock and poor health (indicated by a high proportion of the load rejected at the plant for traumatic injury and disease state). However, the data indicate that by taking risk factors into consideration it is possible to transport hens up to 960 km with low losses in temperate conditions. Mean levels of on‐farm mortality, during the laying period, for a total of 1486 flocks were significantly lower in cages (5.39 per cent) than in barn (8.55 per cent), free‐range (9.52 per cent) or organic flocks (8.68 per cent) according to producer records a median of seven days before depopulation, with considerable variation between flocks in all systems.
Preparation and application of copper hydroxide (oxide) / multiwalled carbon nanotubes nanocomposite-modified glassy carbon electrode as a nitrite sensor
Authors: Liu Youqin , Tian Xiuying , Yan Yun , Xu Yuehua 
Published: June 2012

Copper hydroxide (oxide) / multiwalled carbon nanotubes nanocomposite-modified glassy carbon electrode (Cuhydroxide/oxide/MWCNTs/GCE) was successfully prepared by a novel film plating / cyclic voltammetry (CV) method, which exhibited marked synergistic catalytic effects on the electro-oxidation of NO2–. The preparation process, impedance behavior, and surface morphology of Cuhydroxide/oxide/MWCNTs/GCE were investigated by CV, electrochemical impedance spectroscopy, and scanning electron microscopy (SEM), respectively. The optimum preparation and test conditions for the electrode to have high catalytic activity were acquired. The linear range between the anodic current (Δia, µA) and NO2– concentration (c, mmol L−1) was 1.0 × 10−4 to 14.0 mmol L−1, and the corresponding calibration curve was Δia = −0.1504 + 57.954c (R = 0.9990, n = 32) with a detection limit of 0.03 µmol L−1 (S/N = 3). The Cuhydroxide/oxide/MWCNTs/GCE prepared in the optimal conditions showed good stability, high selectivity, low detection limit, and quick response (<0.2 s) to NO2– in phosphate buffer (pH 5.29) at 0.78 V, which has been successfully applied to the determination of NO2– in pickled vegetables with satisfactory results.
Effect of some surfactants on the chemiluminescent reactions of bis(2,4,6-trichlorophenyl)oxalate and bis(2-nitrophenyl)oxalate with hydrogen peroxide
Authors: Dantoni Patrícia , Rodrigues Ana Clara B. , Matsuda Margareth Mie N. , Coichev Nina 
Published: June 2012

The chemiluminescent reactions of bis(2,4,6-trichlorophenyl)oxalate (TCPO) and bis(2-nitrophenyl)oxalate (2-NPO) with hydrogen peroxide in acetonitrile/water micellar systems (anionic, cationic, and non-ionic) and γ-cyclodextrin were studied in the presence of fluoranthene or 9,10-diphenylanthracene, imidazole, and two buffer solutions, HTRIS+/TRIS and H2PO4–/HPO42–. The relative chemiluminenscence (CL) intensity is higher in the presence of the cationic (DDAB, CTAC, DODAC, and OTAC), anionic (SDS), and non-ionic (Tween 80) surfactants. In the presence of some non-ionic surfactants (Brij 35, Brij 76, and Tween 20), the CL intensity was partially quenched compared with the reaction with no surfactant. The sensitivity for hydrogen peroxide determination in the range 0.01 × 10−4 to 1.0 × 10−4 mol L–1, considering the slope of the calibration curves (maximum peak height of CL vs. concentration), improved with the introduction of DDAH, CTAB, and SDS in HTRIS+/TRIS buffer.
Highly diastereoselective Mannich-type reaction of titanium enolate derived from 2′-hydroxypropiophenone
Authors: Deng Guisheng , Mo Honghua , Luo Jing , Zou Jingyuan 
Published: June 2012

Mannich-type addition of the titanium enolate derived from 2′-hydroxypropiophenone to N-tosylimines provided the corresponding β-aminoketones in good to excellent yields (60%–90%) and with >99 : <1 anti diastereoselectivity. A model for synthesis of anti β-aminoketone as a predominant isomer based on the titanium enolate has been developed. High anti selectivity is not independent of the electron-donating and electron-withdrawing groups on the phenyl ring of the N-tosylimines. Noteworthy is the observation that the influence of a second Lewis acid on the Mannich-type reaction based on the titanium enolate is opposite to that obtained from other titanium enolates reported. A reasonable mechanism has been proposed to explain the high anti diastereoselectivity and the effect of a second Lewis acid on yield.
Markers of the APC/β-Catenin Signaling Pathway as Potential Treatable, Preneoplastic Biomarkers of Risk for Colorectal Neoplasms
Authors: Ahearn Thomas U. , Shaukat Aasma , Flanders W. Dana , Seabrook March E. , Bostick Roberd M. 
Published: June 2012

AbstractBackground: Malfunctioning of the adenomatous polyposis coli (APC)/β-catenin signaling pathway is both an early and common event in sporadic colorectal cancer. To assess the potential of APC/β-catenin signaling pathway markers as treatable, preneoplastic biomarkers of risk for colorectal neoplasms, we conducted a pilot colonoscopy-based case–control study (51 cases and 154 controls) of incident, sporadic colorectal adenoma.Methods: We evaluated APC, β-catenin, and E-cadherin expression in normal mucosa from the rectum and ascending and sigmoid colon using automated immunohistochemical and quantitative image analysis. Diet, lifestyle, and medical history were assessed with validated questionnaires.Results: In the normal rectal mucosa, the ratio of the proportion of APC expression in the upper 40% of crypts with total β-catenin expression (APC/β-catenin score) was 14.3% greater in controls than in cases [P = 0.02; OR, 0.40; 95% confidence interval (CI), 0.14–1.14]. Compared with controls, in cases, APC expression was 3.2% lower, β-catenin expression was 3.0% higher, and E-cadherin expression was 0.7% lower; however, none of these differences were statistically significant. The APC/β-catenin score statistically significantly differed according to categories of plausible risk factors for colorectal cancer [e.g., it was 17.7% higher among those with 25(OH) vitamin D3 concentrations ≥ 27 ng/mL].Conclusions: These preliminary data suggest that the combined expression of APC and β-catenin in the normal rectal mucosa may be associated with risk for incident, sporadic colorectal neoplasms, as well as with modifiable risk factors for colorectal neoplasms.Impact: Our results may help advance the development of treatable, preneoplastic biomarkers of risk for colorectal neoplasms. Cancer Epidemiol Biomarkers Prev; 21(6); 969–79. ©2012 AACR.
MEDI0639: A Novel Therapeutic Antibody Targeting Dll4 Modulates Endothelial Cell Function and Angiogenesis In Vivo
Authors: Jenkins David W. , Ross Sarah , Veldman-Jones Margaret , Foltz Ian N. , Clavette Brandon C. , Manchulenko Kathy , Eberlein Cath , Kendrew Jane , Petteruti Philip , Cho Song , Damschroder Melissa , Peng Li , Baker Dawn , Smith Neil R. , Weir Hazel M. , Blakey David C. , Bedian Vahe , Barry Simon T. 
Published: June 2012

Abstract
The Notch signaling pathway has been implicated in cell fate determination and differentiation in many tissues. Accumulating evidence points toward a pivotal role in blood vessel formation, and the importance of the Delta-like ligand (Dll) 4-Notch1 ligand–receptor interaction has been shown in both physiological and tumor angiogenesis. Disruption of this interaction leads to a reduction in tumor growth as a result of an increase in nonfunctional vasculature leading to poor perfusion of the tumor. MEDI0639 is an investigational human therapeutic antibody that targets Dll4 to inhibit the interaction between Dll4 and Notch1. The antibody cross-reacts to cynomolgus monkey but not mouse species orthologues. In vitro MEDI0639 inhibits the binding of Notch1 to Dll4, interacting via a novel epitope that has not been previously described. Binding to this epitope translates into MEDI0639 reversing Notch1-mediated suppression of human umbilical vein endothelial cell growth in vitro. MEDI0639 administration resulted in stimulation of tubule formation in a three-dimensional (3D) endothelial cell outgrowth assay, a phenotype driven by disruption of the Dll4-Notch signaling axis. In contrast, in a two-dimensional endothelial cell–fibroblast coculture model, MEDI0639 is a potent inhibitor of tubule formation. In vivo, MEDI0639 shows activity in a human endothelial cell angiogenesis assay promoting human vessel formation and reducing the number of vessels with smooth muscle actin-positive mural cells coverage. Collectively, the data show that MEDI0639 is a potent modulator of Dll4-Notch signaling pathway. Mol Cancer Ther; 11(8); 1650–60. ©2012 AACR.
GSK-3 Promotes Cell Survival, Growth, and PAX3 Levels in Human Melanoma Cells
Authors: Kubic Jennifer D. , Mascarenhas Joseph B. , Iizuka Takumi , Wolfgeher Don , Lang Deborah 
Published: June 2012

Abstract
GSK-3 is a serine/threonine kinase involved in a diverse range of cellular processes. GSK-3 exists in two isoforms, GSK-3α and GSK-3β, which possess some functional redundancy but also play distinct roles depending on developmental and cellular context. In this article, we found that GSK-3 actively promoted cell growth and survival in melanoma cells, and blocking this activity with small-molecule inhibitor SB216763 or gene-specific siRNA decreased proliferation, increased apoptosis, and altered cellular morphology. These alterations coincided with loss of PAX3, a transcription factor implicated in proliferation, survival, and migration of developing melanoblasts. We further found that PAX3 directly interacted with and was phosphorylated in vitro on a number of residues by GSK-3β. In melanoma cells, direct inhibition of PAX3 lead to cellular changes that paralleled the response to GSK-3 inhibition. Maintenance of PAX3 expression protected melanoma cells from the anti-tumor effects of SB216763. These data support a model wherein GSK-3 regulates proliferation and morphology of melanoma through phosphorylation and increased levels of PAX3. Mol Cancer Res; 10(8); 1065–76. ©2012 AACR.
A Novel 19q13 Nucleolar Zinc Finger Protein Suppresses Tumor Cell Growth through Inhibiting Ribosome Biogenesis and Inducing Apoptosis but Is Frequently Silenced in Multiple Carcinomas
Authors: Cheng Yingduan , Liang Pei , Geng Hua , Wang Zhaohui , Li Lili , Cheng Suk Hang , Ying Jianming , Su Xianwei , Ng Ka Man , Ng Margaret H.L. , Mok Tony S.K. , Chan Anthony T.C. , Tao Qian 
Published: June 2012

Abstract
Epigenetic disruption of tumor suppressor genes is frequently involved in tumorigenesis. We identified a novel 19q13 KRAB domain-containing zinc finger protein, ZNF545/ZFP82, broadly expressed in normal tissues but downregulated in multiple tumor cell lines. The ZNF545 promoter contains a CpG island, which is frequently methylated in cell lines. The transcriptional silencing of ZNF545 could be reversed by pharmacologic or genetic demethylation, indicating direct epigenetic silencing. ZNF545 was also frequently methylated in multiple primary tumors of nasopharyngeal, esophageal, lung, gastric, colon, and breast, but rarely in normal epithelial tissues and paired normal tissues. ZNF545 is located in the nucleus and mainly sequestered in nucleoli, functioning as a repressor. ZNF545 is able to repress NF-κB and AP-1 signaling pathways, whereas ectopic expression of ZNF545 in silenced tumor cells significantly inhibited their growth and induced apoptosis. Functional studies showed that ZNF545 was involved in ribosome biogenesis through inhibiting the activity of rDNA promoter and decreasing cellular protein translation efficiency. Thus, we identified ZNF545 as a novel tumor suppressor inducing tumor cell apoptosis, repressing ribosome biogenesis and target gene transcription. The tumor-specific methylation of ZNF545 could be an epigenetic biomarker for cancer diagnosis. Mol Cancer Res; 10(7); 925–36. ©2012 AACR.
Dietary Methyl Donor Depletion Protects Against Intestinal Tumorigenesis in Apc
Min/+ Mice
Authors: Kadaveru Krishna , Protiva Petr , Greenspan Emily J. , Kim Young-In , Rosenberg Daniel W. 
Published: June 2012

Abstract
Despite recent population data, the influence of dietary folate supplementation on colon cancer risk remains controversial. This study examines the effects of folate deficiency, in combination with choline, methionine, and vitamin B12 depletion, on intestinal tumorigenesis in ApcMin/+ mice. Methyl donor sufficient (MDS) and deficient (MDD) diets were started at five or 10 weeks of age and tumors evaluated at 16 weeks. MDD suppressed intestinal tumor formation in ApcMin/+ mice (∼80%) when started at five weeks of age. The protective effect was lost when MDD was initiated at 10 weeks of age, indicating an important time dependency on cancer suppression. Concomitant with cancer protection, MDD restricted body weight gain. Therefore, a second study was conducted in which MDS was given ad libitum or pair-fed with MDD. Although small intestinal tumors were reduced 54% in pair-fed MDS mice, MDD caused a further reduction (96%). In colon, although MDD did not affect tumor numbers, tumor size was reduced. Gene expression profiling of normal-appearing colonic mucosa after 11 weeks on MDD identified a total of 493 significantly downregulated genes relative to the MDS group. Pathway analysis placed many of these genes within general categories of inflammatory signaling and cell-cycle regulation, consistent with recently published human data obtained during folate depletion. Further studies are warranted to investigate the complex interplay of methyl donor status and cancer protection in high-risk populations. Cancer Prev Res; 5(7); 911–20. ©2012 AACR.