bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patch test reaction on Ethiopian subjects with eczema
Authors: Bilcha Kassahun Desalegn , Shibeshi Dagnachew , Grangsjo Anders , Hiletework Mengistu 
Published: August 2009

AbstractBackground  Allergic contact dermatitis is a common condition with an incidence of 1–10% in the general population. An increasing number of allergens in the environment are responsible for the condition. These allergens can be identified using patch testing. Many countries have a standard series of common allergens used for patch testing. There is no standard series of allergens in Ethiopia, and our objective was to obtain baseline data for common allergens for future standardization.Methods  One hundred and eighty‐one subjects with eczema were patch tested using 17 selected allergens from Chemotechnique Diagnostics AB employing a standard procedure.Results  Positive patch test reactions were detected in more than 60% of subjects, the most common allergen being nickel, followed by fragrance mix and butylphenolformaldehyde. A higher incidence of positive reactions was seen in females.Conclusions  A high incidence of positive patch test reactions was identified in the study population, and the introduction of patch testing in Ethiopia is essential for the management of allergic contact dermatitis.
Amoxicillin–clavulanic acid‐induced linear immunoglobulin A bullous dermatosis: case report and review of the literature
Authors: Panasiti Vincenzo , Rossi Mariarita , Devirgiliis Valeria , Curzio Michela , Bottoni Ugo , Calvieri Stefano 
Published: August 2009

AbstractBackground Linear immunoglobulin A bullous dermatosis (LABD) is an autoimmune subepidermal blistering disease, rarely induced by drugs.Methods We describe a case of a 47‐year‐old man who developed a severe blistering eruption after therapy with amoxicillin–clavulanic acid.Results Histopathologic examination and direct immunofluorescence were consistent with a diagnosis of LABD. Therapy with dapsone controlled the disease but, after sun exposure, there was a worsening of the illness.Conclusion To our knowledge, this is the first case of amoxicillin–clavulanic acid‐induced LABD in an adult.
Cutaneous pili migrans: a case report and review of the literature
Authors: Luo Di‐Qing , Liu Jun‐Hua , Huang Ying‐Biao , He Ding‐Yang , Zhang Hai‐Yan 
Published: August 2009

AbstractBackground  Cutaneous pili migrans is a rare condition in which the hair shaft penetrates the superficial layer of the skin and produces a creeping eruption mimicking the lesion of cutaneous larva migrans.Methods  We report a 28‐year‐old Chinese man who presented with a painful and slow‐moving black, linear eruption on the left sole after walking on a waterlogged street wearing slippers without socks.Results  The lesion started at the anterior part of the foot near the external border, extending linearly along Langer's line towards the lateral side. It then turned in a U‐shaped direction and moved across the foot to the other side, where it made a right angle and moved along the internal side of the foot. The lesion was reminiscent of cutaneous larva migrans. Walking made the line move more quickly. Physical examination showed that there was a broken epidermis at the probable entrance and a black thin line at the advancing end of the lesion. The black line was demonstrated to be a hair shaft with a sharp head by a shallow incision of the skin and examination under a microscope. After removal of the hair, the pain disappeared and the lesion diminished immediately, leaving slight pigmentation.Conclusion  The broken epidermis may be one of the main reasons for the hair shaft entering the skin. The force of body motion caused movement, which was made easier by the sharp head of the hair shaft. Hair can produce an eruption reminiscent of cutaneous larva migrans.
Effect of progesterone on gonococci‐induced apoptosis and respiratory burst of human polymorphonuclear leukocytes in vitro
Authors: Chen Hongxiang , Wu Zhihong , Juan Li , Chen Rongyi , Yu Ying , Xu Li , Shuai Jun , Tu Ya‐Ting 
Published: August 2009

AbstractBackground  Progesterone may have clinical relevance in females with asymptomatic gonococcal infections.Aim  To investigate the regulatory effect of progesterone on apoptosis and oxidative burst activity of polymorphonuclear leukocytes (PMNs) challenged by Neisseria gonorrhoeae in vitro.Methods  Isolated PMNs were incubated with progesterone or staurosporine. Staurosporine was used as a positive control for our in vitro model. Expression levels of inhibitory apoptosis proteins (IAPs), cellular IAP2 (cIAP2), and X‐linked IAP (XIAP) were determined by real‐time polymerase chain reaction (PCR). In addition, PMN apoptosis at various time points (3, 8, 12, and 24 h) was assayed by flow cytometry. Luminol amplified chemiluminescence methods were used to quantify the oxidative burst function of PMNs challenged with N. gonorrhoeae ST2951.Results  cIAP2 was upregulated significantly in PMNs with progesterone treatment in 3 h, and XIAP was upregulated slightly compared with the medium + ST2951 group, whereas cIAP2 was downregulated in staurosporine‐challenged PMNs. In addition, we found that progesterone delayed the onset of apoptosis activity in N. gonorrhoeae ST2951‐challenged PMNs, notably at 12 h. No statistically significant changes in PMN oxidative burst activity were observed at 10 ng/mL of progesterone. Staurosporine enhanced the production of superoxide anion (respiratory burst) of human PMNs stimulated by N. gonorrhoeae.Conclusion  Progesterone plays an important regulatory role in the interaction of PMNs and N. gonorrhoeae. Delayed PMN apoptosis induced by progesterone presumably acts as a mechanism for N. gonorrhoeae to avoid the innate immune response and establish long‐term, low‐level infection in the female reproductive tract.
Parental anxiety before and after food challenges in children with suspected peanut and hazelnut allergy
Authors: Zijlstra Wieneke T. , Flinterman Annebeth E. , Soeters Lotte , Knulst André C. , Sinnema Gerben , L'Hoir Monique P. , Pasmans Suzanne G. 
Published: August 2009

Zijlstra WT, Flinterman AE, Soeters L, Knulst AC, Sinnema G, L’Hoir MP, Pasmans SG. Parental anxiety before and after food challenges in children with suspected peanut and hazelnut allergy. Pediatr Allergy Immunol 2010: 21: e439–e445. © 2009 John Wiley & Sons A/SAs ingestion of peanut and hazelnut by allergic children is potentially life threatening, parents of these children need to be vigilant about their child’s dietary intake. This may cause high levels of anxiety. To assess parental anxiety about food‐allergic reaction in their child (state anxiety) and their personal disposition to anxiety (trait anxiety). Parental anxiety was investigated again after food challenges. Fifty‐seven children (3–16 yr, mean age 7.2) with suspected peanut or hazelnut allergy (mean specific IgE 20.9) were evaluated by double‐blind, placebo‐controlled food challenge (DBPCFC). Thirty‐two children (56%) developed an allergic reaction. All parents completed the Spielberger State‐Trait Anxiety Inventory (STAI) prior to DBPCFC and 2 wk, 3 months and 1 yr thereafter. The mean anxiety scores on these moments were compared with each other and with general Dutch norms. The STAI was also investigated in a group that refused DBPCFC. Prior to DBPCFC, parents had high levels of state anxiety in contrast to a lower trait anxiety compared to the norm group. After DBPCFC, the state anxiety was significantly lower, regardless of a positive or negative outcome (p ≤ 0.05). The state anxiety was still significant lower after 1 yr (p ≤ 0.03). The trait anxiety remained unchanged in mothers and slightly decreased in fathers. The state anxiety in the group that refused DBPCFC was comparable to the challenge group, but the trait anxiety was significantly higher (p = 0.038). Parents of children with suspected peanut or hazelnut allergy show high levels of anxiety about a food‐allergic reaction. After DBPCFC, the anxiety was significantly lower, even in the group with a positive outcome.
Plasmapheresis treatment of antibody‐mediated rejection in an A2 donor to O pediatric liver transplant recipient
Authors: Martin T. , Schwartz J. , Demetris A. , Comstock J. , Lowichik A. , Book L. 
Published: August 2009

Martin T, Schwartz J, Demetris A, Comstock J, Lowichik A, Book L. Plasmapheresis treatment of antibody‐mediated rejection in an A2 donor to O pediatric liver transplant recipient. Pediatr Transplantation 2011: 15:E15–E18. © 2009 John Wiley & Sons A/S.Abstract:  It is safe to transplant kidneys from blood group A2 donors into O recipients if the latter have low titers of anti‐A antibodies. However, in liver transplantation, O and B recipients of A2 donor livers are not routinely screened for anti‐blood group antibodies because of the immuno‐absorptive capacity of the liver and the low incidence of antibody‐mediated rejection. Herein, we report a rare case of combined cell and antibody‐mediated rejection in a pediatric blood group O recipient of an A2 donor liver, and rescue of the allograft using PP and IVIG.
The efficiency of purifying selection in Mammals vs. Drosophila for metabolic genes
Authors: PETIT N. , BARBADILLA A. 
Published: August 2009

AbstractThe nearly neutral theory of molecular evolution states that the efficiency of natural selection depends on the effective population size. By using a wide range of multispecies data on nucleotide polymorphism, we have tried to ascertain whether there are any differences in the level of selective constraints of metabolic process genes between Mammals and Drosophila species. The results are consistent with a higher selective constraint in Drosophila than in Mammals, according to the expected under the nearly neutral model: purifying selection seems to be more efficient in species with a larger effective population size.
The effect of pathogens on selection against deleterious mutations in Drosophila melanogaster
Authors: YOUNG JADENE A. , YOURTH CHRISTOPHER P. , AGRAWAL ANEIL F. 
Published: August 2009

AbstractIn natural populations, fitness is reduced by both deleterious mutations and parasites. Few studies have examined interactions between these two factors, particularly at the level of individual genes. We examined how the presence of a bacterial pathogen, Pseudomonas aeruginosa, affected the selection against each of eight deleterious mutations in Drosophila melanogaster. We found that mutations tended to become more deleterious in the presence of disease. This increase in the average selection was primarily due to three genes with the remainder showing little evidence of change.
Scanning Electron Microscopic Study of the Lingual Papillae in the Arctic Fox (Alopex lagopus L., 1758)
Authors: Jackowiak H. , Godynicki S. , Skieresz‐Szewczyk K. , Trzcielińska‐Lorych J. 
Published: August 2009

SummaryThis study aims to show the distribution and the three‐dimensional structure of the lingual papillae in the arctic fox. The macro‐ and microscopic structure of the tongue and its lingual papillae was studied in 11 adult arctic foxes. Two types of mechanical papillae were distinguished on the dorsal surface of the tongue – filiform papillae and conical papillae. The gustatory papillae in the arctic fox are represented by fungiform, vallate and foliate papillae. The keratinized filiform papillae on the anterior part of tongue are composed of one big posterior process accompanied by 10–12 secondary anterior processes. The number of anterior processes of filiform papillae undergo a complete reduction within the area between the posterior part of the body of the tongue and area of the vallate papillae. The conical papillae cover the whole dorsal surface of the root of the tongue, including the lateral parts surrounding the area of the vallate papillae and the posterior part of the root. The size of the conical papillae increases towards the root of the tongue but their density decreases. In the arctic fox, there are three pairs of vallate papillae distributed on the plan of a triangle. The diameter of vallate papillae in each successive pair is bigger. The wall surrounding the body of the vallate papilla and its gustatory trench is composed of six to eight conical papillae joined at various degree. The foliate papillae on both margins of the tongue consist of seven to nine laminae.
A Landmark Analysis‐Based Approach to Age and Sex Classification of the Skull of the Mediterranean Monk Seal (Monachus monachus) (Hermann, 1779)
Authors: Brombin C. , Mo G. , Zotti A. , Giurisato M. , Salmaso L. , Cozzi B. 
Published: August 2009

SummaryThis work aimed at applying geometric morphometric analysis techniques to the skull of the Mediterranean monk seal (Monachus monachus, Hermann, 1779). Inferential analyses were performed using a non‐parameteric permutation framework based on a series of skulls of different age classes belonging to individuals of both sexes. Our goal was to establish whether a statistical approach based on osteometric measurements and surface analysis of photographs of the left lateral plane of the skull may lead to a different and scientifically sound method of age and sex classification in this critically endangered marine mammal. Our data indicate that non‐parametric combination methodology enables the researcher to give local assessment using a combination with domains. Developing geometric morphometric techniques in a non‐parametric permutation framework could be useful in solving high dimensional and small sample size problems as well as classification problems, including zoological classification of specimens within a specific population. The Mediterranean monk seal is believed to be the world’s rarest pinniped and one of the most endangered mammals of the world, with fewer than 600 individuals currently surviving. The use of shape analysis would allow new insights into the biological characteristics of the monk seal by simply extracting potentially new information on age and size from museal specimens.
Immunohistochemical Localization of the Progesterone and Oestrogen α Receptors in the Uterine Horns of the African Giant Rat (Cricetomys gambianus)
Authors: Madekurozwa M.‐C. , Oke B. O. , Akinloye A. K. 
Published: August 2009

SummaryThe present study investigated the immunolocalization of the progesterone and oestrogen α receptors in the uterine horns of the African giant rat during the oestrous cycle. The progesterone and oestrogen α receptors were demonstrated in various cellular constituents of the endometrium, myometrium and perimetrium. The intensity of progesterone and oestrogen α receptor immunostaining in the endometrial and myometrial layers of the uterine horns varied during the oestrous cycle. The intensity of oestrogen α receptor immunoreactivity in the luminal epithelium was high during pro‐oestrus, oestrus and dioestrus. Progesterone and oestrogen α receptor immunoreactivity in the endometrial epithelia was absent during metoestrus. Moderate to strong immunostaining for the progesterone and oestrogen α receptors was demonstrated in the myometrial smooth muscle cells during pro‐oestrus, oestrus and dioestrus. The intensity of progesterone and oestrogen α receptor immunostaining in the myometrial smooth muscle cells was low during metoestrus. Stromal cells in the perimetrium consistently expressed progesterone and oestrogen α receptor immunoreactivity throughout the oestrous cycle. The findings of the study indicate that in the giant rat the immunolocalization of the progesterone and oestrogen α receptors, in endometrial and myometrial regions of the uterine horns, varies during the oestrous cycle.
Increased Risk of Conception Failure in German Holstein Friesian Cows with Chronic Endometritis
Authors: Tsousis G , Sharifi AR , Hoedemaker M 
Published: August 2009

ContentsThe objective of this retrospective field study was to evaluate the effect of chronic clinical endometritis (CCE) on days open in German Holstein Friesian cows. Two hundred and sixty‐four cows diagnosed with CCE from day 14 to 42 postpartum were included in this study. A Cox’s proportional hazards regression model with stepwise forward selection for days open was used and the variables type of vaginal discharge, ovarian cysts and a marked loss of body condition score proved to be significant with hazard ratios of 1.5, 2.0 and 1.9 respectively. Although not remaining in the model, a weak positive effect of treatment for CCE and a negative effect of high milk production could also be found in the univariate analysis. These results suggest that the suppression of fertility of animals diagnosed with CCE varies according to the different symptoms, which could be used to support decisions regarding treatment. Management factors such as a marked loss in body condition and high milk production prolonged the number of days open of animals with CCE.
Structural empowerment, job satisfaction, and turnover intention of Chinese clinical nurses
Authors: Cai Chunfeng , Zhou Zongkui 
Published: August 2009

AbstractThe purpose of this study was to examine the levels of workplace structural empowerment perceived by Chinese clinical nurses, as well as to identify the relationship between nurses' perceptions of empowerment and job satisfaction, and turnover intention. A total of 189 staff nurses from two hospitals in central China completed a self‐administered questionnaire. The results indicated that these nurses perceived moderate levels of workplace empowerment. Structural empowerment and job satisfaction were found to be negatively related to turnover intention. These findings have important implications for administrators providing an effective work environment for clinical nurses.
Thai women's experience of intimate partner violence during the perinatal period: A case study analysis
Authors: Saito Amornrat Sricamsuk , Cooke Marie , Creedy Debra K. , Chaboyer Wendy 
Published: August 2009

AbstractThis exploratory descriptive study is part of a larger program that investigated the prevalence and frequency of different forms of intimate partner violence during pregnancy and the immediate post‐partum period among Thai women and the associated maternal and neonatal health outcomes. Two case studies are used to illustrate the different experiences of victims, as well as the barriers to seeking help and the strategies used to manage their circumstances. The study highlights the neglect of women's rights by police and community leaders, the attribution of blame to women, and their perceived sense of powerlessness and a lack of knowledge about the available resources.
Role conflict, role ambiguity, and burnout in nurses and physicians at a university hospital in Turkey
Authors: Tunc Tulin , Kutanis Rana Ozen 
Published: August 2009

AbstractIn many countries currently, health‐care professionals experience burnout in their professional life. This study explored the relationship between burnout, and role conflict and role ambiguity in nurses and physicians at a university hospital in Turkey. The data were collected by questionnaires that included sociodemographic variables, Maslach's Burnout Inventory (MBI), and Rizzo's Role Conflict and Role Ambiguity Scales. Two hundred and fifty one health‐care professionals (170 physicians and 81 nurses) responded to the survey. There was a strong positive correlation between the MBI and Rizzo's Role Conflict and Role Ambiguity Scales. The nurses showed significantly higher levels of role conflict, role ambiguity, and burnout compared to the physicians. A multiple regression analysis showed that role conflict and role ambiguity might help to explain the higher level of burnout experienced by the nurses compared to the physicians. Future research is needed to develop preventive measures for the burnout of health‐care professionals.
Abundance ofZetaproteobacteriawithin crustal fluids in back‐arc hydrothermal fields of the Southern Mariana Trough
Authors: Kato Shingo , Yanagawa Katsunori , Sunamura Michinari , Takano Yoshinori , Ishibashi Jun‐ichiro , Kakegawa Takeshi , Utsumi Motoo , Yamanaka Toshiro , Toki Tomohiro , Noguchi Takuroh , Kobayashi Kensei , Moroi Arimichi , Kimura Hiroyuki , Kawarabayasi Yutaka , Marumo Katsumi , Urabe Tetsuro , Yamagishi Akihiko 
Published: August 2009

SummaryTo extend knowledge of subseafloor microbial communities within the oceanic crust, the abundance, diversity and composition of microbial communities in crustal fluids at back‐arc hydrothermal fields of the Southern Mariana Trough (SMT) were investigated using culture‐independent molecular techniques based on 16S rRNA gene sequences. Seafloor drilling was carried out at two hydrothermal fields, on‐ and off‐ridge of the back‐arc spreading centre of the SMT. 16S rRNA gene clone libraries for bacterial and archaeal communities were constructed from the fluid samples collected from the boreholes. Phylotypes related toThiomicrospirain theGammaproteobacteria(putative sulfide‐oxidizers) andMariprofundusin theZetaproteobacteria(putative iron‐oxidizers) were recovered from the fluid samples. A number of unique archaeal phylotypes were also recovered. Fluorescencein situhybridization (FISH) analysis indicated the presence of active bacterial and archaeal populations in the fluids. TheZetaproteobacteriaaccounted for up to 32% of the total prokaryotic cell number as shown by FISH analysis using a specific probe designed in this study. Our results lead to the hypothesis that theZetaproteobacteriaplay a role in iron oxidation within the oceanic crust.
A distinct population of Saccharomyces cerevisiae in New Zealand: evidence for local dispersal by insects and human‐aided global dispersal in oak barrels
Authors: Goddard Matthew R. , Anfang Nicole , Tang Rongying , Gardner Richard C. , Jun Casey 
Published: August 2009

SummaryHumans have used S. cerevisiae to make alcoholic beverages for at least 5000 years and now this super‐model research organism is central to advances in our biological understanding. Current models for S. cerevisiae suggest that its population comprises distinct domesticated and natural groups as well as mosaic strains, but we generally know little of the forces which shape its population structure. In order to test the roles that ecology and geography play in shaping the S. cerevisiae species we examined nine variable microsatellite loci in 172 strains of S. cerevisiae isolated from two spontaneous grape juice ferments, soil, flowers, apiaries and bark in New Zealand. Bayesian analysis shows that the S. cerevisiae in NZ comprise a subdivided but interbreeding population that out‐crosses ∼20% of the time. Some strains contributing to spontaneous ferments cluster with NZ soil/bark isolates, but others cluster with isolates from French oak barrels. It seems some strains have been globally dispersed by humans in oak barrels while some are locally vectored by insects. These data suggest geography is more important than ecology in shaping S. cerevisiae's population structure.