bluuebunny/crossref_metadata_2025_split huggingface dataset:
Flow injection chemiluminescence determination of 6‐mercaptopurine based on a new system of potassium permanganate–thioacetamide–sodium hexametaphosphate
Authors: Wang Lun , Ling Bo , Chen Hongqi , Liang Ani , Qian Binbin , Fu Jie 
Published: September 2009

AbstractA novel chemiluminescence method for the determination of 6‐mercaptopurine was established based on 6‐mercaptopurine inhibition of the chemiluminescence emission of potassium permanganate–thioacetamide–sodium hexametaphosphate system. The peak height was proportional to log 6‐mercaptopurine concentration in the range 7.0 × 10−10 to 1.0 × 10−7 g/mL and the detection limit was 1.9 × 10−11 g/mL (S/N = 3). The relative standard deviation was 1.5% for the determination of 8.0 × 10−8 g/mL 6‐mercaptopurine (n = 11). The proposed sensor was successfully applied to the analysis of 6‐mercaptopurine in human serum samples. Copyright © 2009 John Wiley & Sons, Ltd.
A DIGE approach for the assessment of differential expression of the CHO proteome under sodium butyrate addition: Effect of Bcl‐xL overexpression
Authors: Baik Jong Youn , Lee Gyun Min 
Published: September 2009

AbstractBcl‐xL, a member of the Bcl‐2 family, is known to inhibit apoptosis of recombinant Chinese hamster ovary (rCHO) cells induced by the addition of sodium butyrate (NaBu), which is used for the elevated expression of recombinant protein. In order to understand the intracellular effects of Bcl‐xL overexpression on CHO cells treated with NaBu, changes to the proteome caused by controlled Bcl‐xL expression in rCHO cells producing erythropoietin (EPO) in the presence of 3 mM NaBu were evaluated using two‐dimensional differential in‐gel electrophoresis (2D‐DIGE) and MS analysis. The consequences of Bcl‐xL overexpression were not limited to the apoptotic signaling pathway. Out of eight proteins regulated significantly by Bcl‐xL overexpression in 3 mM NaBu addition culture, four proteins were related to cell survival (Iq motif‐containing GTPase‐activating protein 1), cell proliferation (dihydrolipoamide‐S‐acetyltransferase, guanine nucleotide binding protein alpha interacting 2), and repair of DNA damage (BRCA and CDKN1A interacting protein). Taken together, a DIGE approach reveals that overexpression of Bcl‐xL not only inhibits apoptosis in the presence of NaBu but also affects cell proliferation and survival in various aspects. Biotechnol. Bioeng. 2010; 105: 358–367. © 2009 Wiley Periodicals, Inc.
Rapid production of recombinant human IgG With improved ADCC effector function in a transient expression system
Authors: van Berkel Patrick H.C. , Gerritsen Jolanda , van Voskuilen Edwin , Perdok Gerrard , Vink Tom , van de Winkel Jan G.J. , Parren Paul W.H.I. 
Published: September 2009

AbstractRapid production of recombinant human IgG with improved antibody dependent cell‐mediated cytotoxicity (ADCC) effector function is presented. The technique employs transient expression of IgG in suspension growing HEK‐293F cells in the presence of the glycosidase inhibitor kifunensine. The procedure takes ∼7 days, provided that expression plasmids encoding the IgG of interest are available. Kifunensine inhibits the N‐linked glycosylation pathway of HEK‐293F cells in the endoplasmatic reticulum, resulting in IgG with oligomannose type glycans lacking core‐fucose. IgG1 transiently produced in kifunensine‐ treated HEK‐293F cells has improved affinity for the FcγRIIIA molecule as measured in an ELISA based assay, and almost eightfold enhanced ADCC using primary peripheral blood mononuclear effector cells. Biotechnol. Bioeng. 2010; 105: 350–357. © 2009 Wiley Periodicals, Inc.
Pathways to psychiatric care in European prison systems
Authors: Dressing Harald , Salize Hans‐Joachim 
Published: September 2009

AbstractThe aims of this study were to describe and analyse the concepts of provision of mental health services for prison inmates in 24 countries in the European Union and the EFTA. Data were gathered by means of a structured questionnaire that was completed by national experts in the participating countries. This article stresses the different organizational models of mental health care for inmates, different legal standards for screening their mental health status and different pathways to psychiatric care and aftercare. The study revealed serious shortcomings. Even the most rudimentary health reporting standards for mental health care in prison are lacking almost everywhere in Europe. Psychiatric screening and assessment procedures at prison entry and during imprisonment differ substantially and do not fulfil recognized quality standards. In many countries, the appointment of inadequately trained staff to perform such screenings increases considerably the risk that mental disorders or psychiatric needs of the inmates will remain undetected. Furthermore, the pathways to care in the case of an acute psychotic episode differ significantly, since referral to prison hospitals, medical prison wards, forensic hospitals, or general psychiatric hospital are used in various combinations depending on different national legal regulations and on the availability of services or other regional circumstances. Therefore, the collaborating experts place the quality of European prison mental health care into serious question. Copyright © 2009 John Wiley & Sons, Ltd.
Conducting research in corrections: challenges and solutions
Authors: Wakai Sara , Shelton Deborah , Trestman Robert L. , Kesten Karen 
Published: September 2009

AbstractResearch in correctional settings has progressed from the exploitation of a vulnerable population in the years prior to 1978 to its current overly protective and restrictive state. With the considerable growth in the numbers of inmates with medical and mental health care needs, developing effective interventions to serve this population is paramount. There now appear to be signs of interest in and support for research with inmates by correctional agencies, academia, and health care organizations. Innes (2003) has articulated the following strategies for implementing research in prison: (1) gather stakeholders; (2) include one or more experienced research collaborators; (3) consider obtaining technical assistance from a university or the National Institute of Corrections (NIC); (4) define potential opportunities/interests that support the institution's mission; (5) develop a relationship with an IRB; (6) pilot a small proposal that is of interest and potential value to the organization. The authors illustrate the implementation of a study in a correctional system that uses these strategies. Copyright © 2009 John Wiley & Sons, Ltd.
Passage of Trojan Peptoids into Plant Cells
Authors: Eggenberger Kai , Birtalan Esther , Schröder Tina , Bräse Stefan , Nick Peter 
Published: September 2009

AbstractEfficient drug delivery is essential for many therapeutic applications. In this context, Trojan peptoids have attracted attention as powerful tools to deliver bioactive molecules into living cells. Certain cell‐penetrating peptides, peptide mimetics, and peptoids have been shown to be endowed with a transport function and the structural features of this function have been characterized. However, most of the research has been done by using mammalian cell cultures as model organisms and the actual cellular mechanism of membrane passage has not been elucidated. Plant cells, which are encased in a cellulosic cell wall and differ in membrane composition, represent an alternative experimental system to address this issue, but so far, have attracted only little attention for both peptide‐ and peptoid‐based carrier systems. Moreover, efficient delivery of nonproteinaceous bioactive macromolecules into living plant cells could complement genetic engineering in biotechnological applications, such as metabolic engineering and molecular farming. In the present study, we investigated carrier peptoids with or without guanidinium side chains with regard to their uptake into plant cells, the cellular mechanism of uptake, and intracellular localization. We can show that in contrast to polyamine peptoids (polylysine‐like) fluorescently labeled polyguanidine peptoids (polyarginine‐like) enter rapidly into tobacco BY‐2 cells without affecting the viability of these cells. A quantitative comparison of this uptake with endocytosis of fluorescently labeled dextranes indicates that the main uptake of the guanidinium peptoids occurs between 30–60 min after the start of incubation and clearly precedes endocytosis. Dual visualization with the endosomal marker FM4‐64 shows that the intracellular guanidinium peptoid is distinct from endocytotic vesicles. Once the polyguanidine peptoids have entered the cell, they associate with actin filaments and microtubules. By pharmacological manipulation of the cytoskeleton we tested whether the association with the cytoskeleton is necessary for uptake, and observed that the actin inhibitor latrunculin B as well as the microtubule inhibitor oryzalin impaired uptake and intracellular spread of the guanidinium carrier to a certain extent. These findings are discussed with respect to the potential mechanisms of uptake and with respect to the potential of Trojan peptoids as tools for metabolic engineering in plant biotechnology.
Cooperative Effect of a Classical and a Weak Hydrogen Bond for the Metal‐Induced Construction of a Self‐Assembled β‐Turn Mimic
Authors: Laungani Andy C. , Keller Manfred , Slattery John M. , Krossing Ingo , Breit Bernhard 
Published: September 2009

AbstractA novel metal‐induced template for the self‐assembly of two independent phosphane ligands by means of unprecedented multiple noncovalent interactions (classical hydrogen bond, weak hydrogen bond, metal coordination, π‐stacking interaction) was developed and investigated. Our results address the importance and capability of weak hydrogen bonds (WHBs) as important attractive interactions in self‐assembling processes based on molecular recognition. Together with a classical hydrogen bond, WHBs may serve as promoters for the specific self‐assembly of complementary monomeric phosphane ligands into supramolecular hybrid structures. The formation of an intermolecular CH⋅⋅⋅N hydrogen bond and its persistence in the solid state and in solution was studied by X‐ray crystal analysis, mass spectrometry and NMR spectroscopy analysis. Further evidence was demonstrated by DFT calculations, which gave specific geometric parameters for the proposed conformations and allowed us to estimate the energy involved in the hydrogen bonds that are responsible for the molecular recognition process. The presented template can be regarded as a new type of self‐assembled β‐turn mimic or supramolecular pseudo amino acid for the nucleation of β‐sheet structures when attached to oligopeptides.
The Implications of (2S,4S)‐Hydroxyproline 4‐O‐Glycosylation for Prolyl Amide Isomerization
Authors: Owens Neil W. , Lee Adrian , Marat Kirk , Schweizer Frank 
Published: September 2009

AbstractThe conformations of peptides and proteins are often influenced by glycans O‐linked to serine (Ser) or threonine (Thr). (2S,4R)‐4‐Hydroxyproline (Hyp), together with L‐proline (Pro), are interesting targets for O‐glycosylation because they have a unique influence on peptide and protein conformation. In previous work we found that glycosylation of Hyp does not affect the N‐terminal amide trans/cis ratios (Ktrans/cis) or the rates of amide isomerization in model amides. The stereoisomer of Hyp—(2S,4S)‐4‐hydroxyproline (hyp)—is rarely found in nature, and has a different influence both on the conformation of the pyrrolidine ring and on Ktrans/cis. Glycans attached to hyp would be expected to be projected from the opposite face of the prolyl side chain relative to Hyp; the impact this would have on Ktrans/cis was unknown. Measurements of 3J coupling constants indicate that the glycan has little impact on the Cγ‐endo conformation produced by hyp. As a result, it was found that the D‐galactose residue extending from a Cγ‐endo pucker affects both Ktrans/cis and the rate of isomerization, which is not found to occur when it is projected from a Cγ‐exo pucker; this reflects the different environments delineated by the proline side chain. The enthalpic contributions to the stabilization of the trans amide isomer may be due to disruption of intramolecular interactions present in hyp; the change in enthalpy is balanced by a decrease in entropy incurred upon glycosylation. Because the different stereoisomers—Hyp and hyp—project the O‐linked carbohydrates in opposite spatial orientations, these glycosylated amino acids may be useful for understanding of how the projection of a glycan from the peptide or protein backbone exerts its influence.
Highly Emissive Diborylphenylene‐Containing Bis(phenylethynyl)benzenes: Structure–Photophysical Property Correlations and Fluoride Ion Sensing
Authors: Zhao Cui‐Hua , Sakuda Eri , Wakamiya Atsushi , Yamaguchi Shigehiro 
Published: September 2009

AbstractA series of 2,5‐bis(dimesitylboryl)‐1,4‐bis(arylethynyl)benzenes 1–6 that contain various p‐substituents on the terminal benzene rings, including NPh2 (1), OMe (2), Me (3), H (4), CF3 (5), and CN (6) groups, were synthesized, and the effects of the p‐substituents on the absorption and fluorescence properties were investigated both in solution and in the solid state. Linear relationships were obtained not only between the Hammett σp+ constants of the p‐substituents and the absorption and fluorescence maxima, quantum yields, and excited‐state dynamics parameters in solution, but also between the σp+ constants and the fluorescence quantum yields in the solid state. An important finding extracted from these results is that the suppressed fluorescence quenching in the solid state is a common feature for the present laterally boryl‐substituted π‐conjugated skeletons. Hence, the diborylphenylene can serve as a useful core unit to develop highly emissive organic solids. In fact, most of the derivatives showed more intense emission in the solid state than in solution. In addition to these studies, the titration experiment of 1 by the addition of nBu4NF was conducted, which showed the stepwise bindings of two fluoride ions with high association constants as well as a drastic change in the fluorescence spectra, while constantly maintaining high quantum yields (0.61–0.76), irrespective of the binding modes. This result also demonstrated the potential utility of the present molecules as an efficient fluorescent fluoride ion sensor.
Two Regioisomers of Endohedral Pyrrolidinodimetallofullerenes M2@Ih‐C80(CH2)2NTrt (M=La, Ce; Trt=trityl): Control of Metal Atom Positions by Addition Positions
Authors: Yamada Michio , Okamura Mayuko , Sato Satoru , Someya Chika I. , Mizorogi Naomi , Tsuchiya Takahiro , Akasaka Takeshi , Kato Tatsuhisa , Nagase Shigeru 
Published: September 2009

AbstractThe two regioisomers of endohedral pyrrolidinodimetallofullerenes M2@Ih‐C80(CH2)2NTrt (M=La, Ce; Trt=trityl) were synthesized, isolated, and characterized. X‐ray crystallographic analyses of [6,6]‐La2@Ih‐C80(CH2)2NTrt and [6,6]‐Ce2@Ih‐C80(CH2)2NTrt revealed that the encapsulated metal atoms are located at the slantwise positions on the mirror plane that parallels the pyrrolidine ring. Paramagnetic NMR analyses of [6,6]‐ and [5,6]‐Ce2@Ih‐C80(CH2)2NTrt were also carried out to clarify the metal positions. As for the [6,6]‐adduct, the metal positions obtained by paramagnetic NMR analysis agree well with the X‐ray structure. In contrast, paramagnetic NMR analysis of the [5,6]‐adduct showed that the two Ce atoms are collinear with the pyrrolidine ring. We also compared the observed paramagnetic effects of the pyrrolidinodimetallofullerenes with those of other cerium‐encapsulating fullerene derivatives such as bis‐silylated Ce2@Ih‐C80 and a carbene adduct of Ce2@Ih‐C80. We found that the metal positions can be explained by the electrostatic potential maps of the corresponding [6,6]‐ and [5,6]‐adducts of [Ih‐C80(CH2)2NTrt]6−. These findings clearly show that metal positions inside fullerene cages can be controlled by means of the addition positions of the addends. In addition, the radical anions of the pyrrolidinodimetallofullerenes were prepared by bulk controlled‐potential electrolysis and characterized by X‐band EPR spectral study.
2,2′‐Bipyrimidine as Efficient Sensitizer of the Solid‐State Luminescence of Lanthanide and Uranyl Ions from Visible to Near‐Infrared
Authors: Zucchi Gaël , Maury Olivier , Thuéry Pierre , Gumy Frédéric , Bünzli Jean‐Claude G. , Ephritikhine Michel 
Published: September 2009

AbstractTreatment of Ln(NO3)3⋅nH2O with 1 or 2 equiv 2,2′‐bipyrimidine (BPM) in dry THF readily afforded the monometallic complexes [Ln(NO3)3(bpm)2] (Ln=Eu, Gd, Dy, Tm) or [Ln(NO3)3(bpm)2]⋅THF (Ln=Eu, Tb, Er, Yb) after recrystallization from MeOH or THF, respectively. Reactions with nitrate salts of the larger lanthanide ions (Ln=Ce, Nd, Sm) yielded one of two distinct monometallic complexes, depending on the recrystallization solvent: [Ln(NO3)3(bpm)2]⋅THF (Ln=Nd, Sm) from THF, or [Ln(NO3)3(bpm)(MeOH)2]⋅MeOH (Ln=Ce, Nd, Sm) from MeOH. Treatment of UO2(NO3)2⋅6H2O with 1 equiv BPM in THF afforded the monoadduct [UO2(NO3)2(bpm)] after recrystallization from MeOH. The complexes were characterized by their crystal structure. Solid‐state luminescence measurements on these monometallic complexes showed that BPM is an efficient sensitizer of the luminescence of both the lanthanide and the uranyl ions emitting visible light, as well as of the YbIIIion emitting in the near‐IR. For Tb, Dy, Eu, and Yb complexes, energy transfer was quite efficient, resulting in quantum yields of 80.0, 5.1, 70.0, and 0.8 %, respectively. All these complexes in the solid state were stable in air.
Synthesis and Base Pairing Properties of 1′,5′‐Anhydro‐L‐Hexitol Nucleic Acids (L‐HNA)
Authors: D'Alonzo Daniele , Van Aerschot Arthur , Guaragna Annalisa , Palumbo Giovanni , Schepers Guy , Capone Stefania , Rozenski Jef , Herdewijn Piet 
Published: September 2009

AbstractOligonucleotides composed of 1′,5′‐anhydro‐arabino‐hexitol nucleosides belonging to the L series (L‐HNA) were prepared and preliminarily studied as a novel potential base‐pairing system. Synthesis of enantiopure L‐hexitol nucleotide monomers equipped with a 2′‐(N6‐benzoyladenin‐9‐yl) or a 2′‐(thymin‐1‐yl) moiety was carried out by a de novo approach based on a domino reaction as key step. The L oligonucleotide analogues were evaluated in duplex formation with natural complements as well as with unnatural sugar‐modified oligonucleotides. In many cases stable homo‐ and heterochiral associations were found. Besides Tm measurements, detection of heterochiral complexes was unambiguously confirmed by LC‐MS studies. Interestingly, circular dichroism measurements of the most stable duplexes suggested that L‐HNA form left‐handed helices with both D and L oligonucleotides.
Structural Model for the Binding Sites of Allosterically Potentiating Ligands on Nicotinic Acetylcholine Receptors
Authors: Luttmann Edgar , Ludwig Jürgen , Höffle‐Maas Anja , Samochocki Marek , Maelicke Alfred , Fels Gregor 
Published: September 2009

AbstractCurrent treatments of Alzheimer's disease include the allosteric potentiation of nicotinic acetylcholine receptor (nAChR) response. The location of the binding site for allosteric potentiating ligands (APLs) within the receptor is not yet fully understood. Based on homology models for the ligand binding domain of human α7, human α4β2, and chicken α7 receptors, as well as blind docking experiments with galanthamine, physostigmine, codeine, and 5HT, we identified T197 as an essential element of the APL binding site at the outer surface of the ligand binding domain (LBD) of nAChR. We also found the previously known galanthamine binding site in the region of K123 at the inside of the receptor funnel, which, however, was shown to not be part of the APL site. Our results are verified by site‐directed mutagenesis and electrophysiological experiments, and suggest that APL and ACh bind to different sites on nicotinic receptors and that allosteric potentiation may arise from a direct interplay between both these sites.
Detection of hematogenous bone metastasis in cervical cancer
Authors: Liu Feng‐Yuan , Yen Tzu‐Chen , Chen Min‐Yu , Lai Chyong‐Huey , Chang Ting‐Chang , Chou Hung‐Hsueh , Hong Ji‐Hong , Chen Yu‐Ruei , Ng Koon‐Kwan 
Published: September 2009

AbstractBACKGROUND:In this large‐scale, retrospective study, the authors evaluated the diagnostic performances of computed tomography (CT), magnetic resonance (MR) imaging, and 18F‐fluorodeoxyglucose‐positron emission tomography (18F‐FDG–PET) in detecting hematogenous bone metastasis in patients with cervical cancer. The associated risk factors also were analyzed.METHODS:Patients with invasive cervical cancer who had both 18F‐FDG–PET studies and CT or MR imaging studies were selected. Patients who had either International Federation of Gynecology and Obstetrics (FIGO) stage III/IV disease or positive lymph node metastasis at the time of primary staging and patients who had suspected recurrent disease were included in the analyses. The diagnostic performances of PET was compared with the performance of CT and MR imaging by using the area under the receiver‐operating‐characteristic curve (AUC). Both univariate and multivariate analyses were applied to assess the risk factors for hematogenous bone metastasis at primary staging.RESULTS:PET was more sensitive than CT (P = .004) and was more specific than MR imaging (P = .04). The diagnostic performance of PET was significantly superior to the performance CT (AUC, 0.964 vs 0.662; P < .001) and MR (AUC, 0.966 vs 0.833; P = .033). Both FIGO stage and the extent of lymph node metastases were associated with hematogenous bone metastasis in univariate analysis. However, the extent of lymph node metastases was the only significant risk factor in multivariate analysis (P = .025).CONCLUSIONS:The current study demonstrated the superiority of 18F‐FDG–PET over CT and MR imaging for detecting hematogenous bone metastasis in patients with advanced cervical cancer. Hematogenous bone metastasis in cervical cancer was associated with the extent of lymph node metastases rather than with FIGO stage. Cancer 2009. © 2009 American Cancer Society.
Interactive direct volume rendering on desktop multicore processors
Authors: Wang Qin , JaJa Joseph 
Published: September 2009

AbstractWe present a new multithreaded implementation for the computationally demanding direct volume rendering (DVR) of volumetric data sets on desktop multicore processors using ray casting. The new implementation achieves interactive rendering of very large volumes, even on high resolution screens. Our implementation is based on a new algorithm that combines an object‐order traversal of the volumetric data followed by a focused ray casting. Using a very compact data structure, our method starts with a quick association of data subcubes with fine‐grain screen tiles appearing along the viewing direction in front‐to‐back order. The next stage uses very limited ray casting on the generated sets of subcubes while skipping empty or transparent space and applying early ray termination in an effective way. Our multithreaded implementation makes use of new dynamic techniques to ensure effective memory management and load balancing. Our software enables a user to interactively explore large data sets through DVR while arbitrarily specifying a 2D transfer function. We test our system on a wide variety of well‐known volumetric data sets on a two‐processor Clovertown platform, each consisting of a Quad‐Core 1.86 GHz Intel Xeon Processor. Our experimental tests demonstrate DVR at interactive rates for the largest data sets that can fit in the main memory on our platform. These tests also indicate a high degree of scalability, excellent load balancing, and efficient memory management across the data sets used. Copyright © 2009 John Wiley & Sons, Ltd.