bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exercise training in obese older adults prevents increase in bone turnover and attenuates decrease in hip bone mineral density induced by weight loss despite decline in bone-active hormones
Authors: Shah Krupa , Armamento-Villareal Reina , Parimi Nehu , Chode Suresh , Sinacore David R , Hilton Tiffany N , Napoli Nicola , Qualls Clifford , Villareal Dennis T 
Published: July 2011

Abstract
Weight loss therapy to improve health in obese older adults is controversial because it causes further bone loss. Therefore, it is recommended that weight loss therapy should include an intervention such as exercise training (ET) to minimize bone loss. The purpose of this study was to determine the independent and combined effects of weight loss and ET on bone metabolism in relation to bone mineral density (BMD) in obese older adults. One-hundred-seven older (age &gt;65 years) obese (body mass index [BMI] ≥30 kg/m2) adults were randomly assigned to a control group, diet group, exercise group, and diet-exercise group for 1 year. Body weight decreased in the diet (−9.6%) and diet-exercise (−9.4%) groups, not in the exercise (−1%) and control (−0.2%) groups (between-group p &lt; 0.001). However, despite comparable weight loss, bone loss at the total hip was relatively less in the diet-exercise group (−1.1%) than in the diet group (−2.6%), whereas BMD increased in the exercise group (1.5%) (between-group p &lt; 0.001). Serum C-terminal telopeptide (CTX) and osteocalcin concentrations increased in the diet group (31% and 24%, respectively), whereas they decreased in the exercise group (−13% and −15%, respectively) (between-group p &lt; 0.001). In contrast, similar to the control group, serum CTX and osteocalcin concentrations did not change in the diet-exercise group. Serum procollagen propeptide concentrations decreased in the exercise group (−15%) compared with the diet group (9%) (p = 0.04). Serum leptin and estradiol concentrations decreased in the diet (−25% and −15%, respectively) and diet-exercise (−38% and −13%, respectively) groups, not in the exercise and control groups (between-group p = 0.001). Multivariate analyses revealed that changes in lean body mass (β = 0.33), serum osteocalcin (β = −0.24), and one-repetition maximum (1-RM) strength (β = 0.23) were independent predictors of changes in hip BMD (all p &lt; 0.05). In conclusion, the addition of ET to weight loss therapy among obese older adults prevents weight loss–induced increase in bone turnover and attenuates weight loss–induced reduction in hip BMD despite weight loss–induced decrease in bone-active hormones. © 2011 American Society for Bone and Mineral Research
Is greater lumbar vertebral BMD associated with more disk degeneration? A study using µCT and discography
Authors: Wang Yue , Boyd Steven K , Battié Michele C , Yasui Yutaka , Videman Tapio 
Published: July 2011

Abstract
It is well documented that osteoarthritis is associated with greater BMD in peripheral extremities. Yet the relationship between vertebral BMD and disk degeneration (DD) remains controversial in the lumbar spine, which may be due largely to the inadequacies of BMD and DD measures. Aiming to clarify the association between vertebral BMD and adjacent DD, we studied 137 cadaveric lumbar vertebrae and 209 corresponding intervertebral disks from the spines of 48 white men aged 21 to 64 years. DD was evaluated using discography. The vertebrae were scanned using a micro–computed tomography (µCT) system to obtain volumetric BMD for the whole vertebra, the vertebral body, the vertebral body excluding osteophytes, and the vertebral body excluding osteophytes and endplates. A random effects model was used to examine the association between the different definitions of vertebral BMD and adjacent DD. No significant association was found between the BMD of the whole vertebra and adjacent DD. However, when the posterior elements were excluded, there was a significant association between greater vertebral body BMD and more severe degeneration in the disk cranial to the vertebra. This association remained after further excluding osteophytes and endplates from the vertebral body BMD measurements. Also, a trend of greater BMD of the vertebral body associated with more adjacent DD was evident. These results clarify the association between vertebral BMD and DD and specifically indicate that it is higher BMD of the vertebral body, not the entire vertebra, that is associated with more severe adjacent DD. This association may be obscured by the posterior elements and is not confounded by osteophytes and endplate sclerosis. © 2011 American Society for Bone and Mineral Research
Mucosal healing of esophageal involvement of Crohn's disease with granulocyte/monocyte adsorption
Authors: Moribata Kosaku , Kato Jun , Iimura Sae , Yoshida Satoshi , Shingaki Naoki , Ueda Kazuki , Deguchi Hisanobu , Inoue Izumi , Maekita Takao , Iguchi Mikitaka , Tamai Hideyuki , Ichinose Masao 
Published: July 2011

AbstractWe report a case of a 16‐year‐old male who suffered from Crohn's disease (CD) with esophageal involvement, showing remarkable improvement with granulocyte/monocyte adsorption (GMA). The patient had been diagnosed as ileocolic CD and was treated with 5‐aminosalicylate. He was admitted to our hospital with symptoms of fever, diarrhea, and odynophagia. Endoscopic examinations revealed that the exacerbation of ileocolic ulcers, and advent of ulcers in esophagus. Because of the patient's refusal to receive corticosteroids, immunomodulators, or biologics, he underwent GMA twice a week. After 10 sessions of GMA, he entered remission with significant decrease in clinical activity. In addition, endoscopic examinations showed remarkable improvement of ileocolic ulcers and disappearance of esophageal lesions. No adverse events were observed. GMA could be effective for manifestations of CD in gastrointestinal tract other than ileum or colon. J. Clin. Apheresis, 2011. © 2011 Wiley‐Liss, Inc.
Outcomes of previously healthy pediatric patients with fulminant sepsis‐induced multisystem organ failure receiving therapeutic plasma exchange
Authors: Qu Lirong , Kiss Joseph E. , Dargo Gina , Carcillo Joseph A. 
Published: July 2011

AbstractBackground: Fulminant sepsis‐induced multisystem organ failure (MSOF) in pediatric patients carries substantial morbidity and mortality. Therapeutic plasma exchange (TPE) has been reported to be beneficial in sepsis‐induced MSOF. We evaluated the outcomes of previously healthy children with fulminant sepsis‐induced MSOF receiving TPE. Materials and Methods: Previously healthy pediatric ICU patients who underwent TPE for MSOF due to fulminant bacterial sepsis were retrospectively reviewed. Eleven patients (three females and eight males) with age ranging 8 months to 14 years were identified (eight meningococcemia and three other infections). All patients received daily TPE with fresh frozen plasma (FFP) as replacement fluid. Organ failure index (OFI—maximum score = 6) was assessed daily for 7 days. Results: A median of 4 TPE (1–14) were performed. Improvements in organ function and platelet count occurred in most patients with 2–4 TPE treatments. All 10 patients who were alive had reduced OFI to 2 by day 7 of initial TPE and were all fully recovered (survival rate = 10/11, 91%). The only death occurred in a patient who died the same day after his first TPE treatment, which was initiated 24 h after development of MSOF. The 10 survivors underwent early initiation of TPE (median 5.3 h) after the onset of MSOF. Conclusions: > TPE may contribute to a better outcome in previously healthy pediatric patients with fulminant sepsis‐induced MSOF, especially if instituted early in the course of multiorgan failure. J. Clin. Apheresis, 2011. © 2011 Wiley‐Liss, Inc.
Urinary κ and λ immunoglobulin light chains in normoalbuminuric type 2 diabetes mellitus patients
Authors: Hassan S.B. , Hanna M.O.F. 
Published: July 2011

AbstractBackground and Aim: Kidney disease is one of the major chronic microvascular complications of diabetes. Tubular involvement may precede glomerular involvement and the appearance of microalbuminuria. The aim of the study was to evaluate quantitatively immunoglobulin light chains (IgLCs), kappa and lambda excretion in the urine of patients with type 2 diabetes mellitus with normoalbuminuria and with microalbuminuria compared to control group. Results: Urinary IgLCs levels of the control group were significantly lower than diabetic patients with normoalbuminuria and diabetic patients with albuminuria. IgLCs were significantly associated with the duration of disease and negatively with estimated glomerular filtration rate. Conclusion: Type 2 diabetic patients can have significantly raised concentrations of urinary IgLCs before microalbuminuria or renal disease occurs. Further investigations are recommended to assess LC evaluation in the early management of diabetic renal disease. J. Clin. Lab. Anal. 25:229–232, 2011. © 2011 Wiley‐Liss, Inc.
TGF‐β1 serum concentration as a complementary diagnostic biomarker of lung cancer: establishment of a cut‐point value
Authors: González‐Santiago Ana E. , Mendoza‐Topete Luz A. , Sánchez‐Llamas Francisco , Troyo‐Sanromán Rogelio , Gurrola‐Díaz Carmen M. 
Published: July 2011

AbstractLung cancer is a malignant disease with increasing mortality rates. Cytokines play a role in normal cell growth regulation and differentiation and are also implicated in malignant disease. Among these cytokines, Transforming Growth Factor β type 1 (TGF‐β1) acts as a tumor promoter in malignant cells. Several clinical studies have found high levels of TGF‐β1 in various cancer types. The aim of this study was to establish a TGF‐β1 cut‐off point as a complementary diagnostic tool in lung cancer detection. Therefore, 72 clinically well‐characterized individuals were studied, 41 lung cancer patients and 31 healthy subjects. Serum TGF‐β1 concentration was measured by an enzyme‐linked immunosorbent assay (ELISA). We compared statistically the serum TGF‐β1 concentration between both groups with analysis of variance, linear regression and receiver operating curve analysis. We observed that lung cancer patients produced higher TGF‐β1 levels than healthy individuals (37,225±9,436 vs. 28,416±9,324 pg/ml, P<0.001). The cut‐point diagnostic value was 30,500 pg/ml with 80.5% sensitivity, 64.5% specificity and odds ratio: 7.5, 95% CI: 2.6–21.8. Conclusions: We found significantly higher TGF‐β1 levels in lung cancer patients than in healthy individuals. We propose the measurement of serum TGF‐β1 levels as a complementary diagnostic test in lung cancer detection. J. Clin. Lab. Anal. 25:238–243, 2011. © 2011 Wiley‐Liss, Inc.
Clinical significance of simultaneous determination of serum tryptophan and tyrosine in patients with lung cancer
Authors: Ren Ya‐Ping , Tang Ai‐Guo , Zhou Qian‐Xuan , Xiang Zhong‐Yuan 
Published: July 2011

AbstractBackground: To explore the clinical significance of serum tyrosine (Tyr) and tryptophan (Trp) in patients with lung cancer, we used a simple and efficient method of high‐performance liquid chromatography with fluorescence detection (HPLC‐FD) that simultaneously measured serum Trp and Tyr contents. Methods: The concentrations of Tyr and Trp were measured simultaneously by HPLC‐FD in the sera of 80 patients with lung cancer and 120 healthy controls. Results: Trp concentrations were significantly lower in patients with lung cancer than in healthy controls (39.26±5.44 vs. 49.93±5.43 µmol/l, respectively; P<0.01), whereas in Tyr concentrations there were no differences with healthy controls (65.38±7.94 vs.66.40±8.55 µmol/l, respectively; P>0.05). In addition, patients in the adenocarcinoma group had significantly lower Trp and Tyr concentrations than those in squamous cell carcinoma group. There was no difference between the early stage and advanced stage of lung cancer. Conclusions: Determination of serum Trp and Tyr concentrations can be employed to assist the diagnosis of the histotypes of lung cancer and tumor stage. Tyr and Trp as indexes on the lung cancer diagnostic sensitivity, specificity were 54.9, 62.9% and 82.4, 92.1%, Trp is an important and special index for lung cancer diagnosis of which the specificity of diagnosis of lung cancer is more than 92%. J. Clin. Lab. Anal. 25:246–250, 2011. © 2011 Wiley‐Liss, Inc.
Ischemia‐modified albumin as a biochemical marker in children with neuroblastoma and soft tissue sarcomas
Authors: Stachowicz‐Stencel Teresa , Synakiewicz Anna , Owczarzak Anna , Sliwińska Aleksandra , Aleksandrowicz‐Wrona Ewa , Lysiak‐Szydowska Wiesawa , Balcerska Anna 
Published: July 2011

AbstractIn this study, the levels of ischemia‐modified albumin (IMA) in pediatric oncology patients with soft tissue sarcomas (STSs) and neuroblastoma (NB) were analyzed. To date, there have been no studies concerning IMA in these groups of patients. Ninety‐nine children with STSs and NB were analyzed from 2006 to 2009, and 30 healthy children were also enrolled in the study. IMA levels were measured throughout treatment in all patients. The levels of IMA in all cancer patients (mean 116.8±39.3 U/ml), in patients with STSs (mean 119.8±27.5 U/ml), and in patients with NB (mean 114.6±36.6 U/ml) were significantly higher than in the control patients (mean 87.3±38.3 U/ml; P=0.0013, 0.0066, and 0.0164, respectively). IMA levels increased before and during the treatment compared with levels in the controls. The determination of IMA levels in pediatric oncology patients with poor prognoses from STSs and NB may play an important role in predicting response to therapy and overall outcome. J. Clin. Lab. Anal. 25:255–258, 2011. © 2011 Wiley‐Liss, Inc.
Evolving methods for single nucleotide polymorphism detection: Factor V Leiden mutation detection
Authors: Oh Herin , Smith Cassandra L. 
Published: July 2011

AbstractBackground: The many techniques used to diagnose the Factor V Leiden (FVL) mutation, the most common hereditary hypercoagulation disorder in Eurasians, and the most frequently requested genetic test reflect the evolving strategies in protein and DNA diagnosis. Methods: Here, molecular methods to diagnose the FVL mutation are discussed. Results: Protein‐based detection assays include the conventional functional activated protein C resistance coagulation test and the recently reported antibody‐mediated sensor detection; and DNA‐based assays include approaches that use electrophoretic fractionation e.g., restriction fragment length polymorphism, denaturing gradient gel electrophoresis, and single‐stranded conformational PCR analysis, DNA hybridization (e.g., microarrays), DNA polymerase‐based assays, e.g., extension reactions, fluorescence polarization template‐directed dye‐terminator incorporation, PCR assays (e.g., amplification‐refractory mutation system, melting curve analysis using real‐time quantitative PCR, and helicase‐dependent amplification), DNA sequencing (e.g., direct sequencing, pyrosequencing), cleavase‐based Invader assay and ligase‐based assays (e.g., oligonucleotide ligation assay and ligase‐mediated rolling circle amplification). Conclusion: The method chosen by a laboratory to diagnose FVL not only depends on the available technical expertise and equipment, but also the type, variety, and extent of other genetic disorders being diagnosed. J. Clin. Lab. Anal. 25:259–288, 2011. © 2011 Wiley‐Liss, Inc.
Correlation of Chlamydia and Chlamydophila spp. IgG and IgM antibodies by microimmunofluorescence with antigen detection methods
Authors: She Rosemary C. , Welch Ryan , Wilson Andrew R. , Davis David , Litwin Christine M. 
Published: July 2011

AbstractCorrelation of serologic titers for Chlamydia trachomatis with other tests has been based on direct fluorescence antibody (DFA) testing and culture, but not on nucleic acid‐based tests that are used for screening. We retrospectively reviewed the specificity of antibodies against C. trachomatis, Chlamydia psittaci, and Chlamydophila pneumoniae by microimmunofluorescence (MIF) when compared with DFA, culture, nucleic acid probe, and transcription‐mediated amplification. Over a 6‐year period, 226 cases had both MIF and one of these other methods performed for comparison. Agreement between C. trachomatis antigen or nucleic acid detection and MIF results was 87% (197/226). C. trachomatis serology had a negative predictive value of 98%, and 10.6% of cases were positive by serology and negative by antigen testing. Of the 13 patients who had a positive C. trachomatis antigen or nucleic acid test result, 9 had IgG and/or IgM titers highest against C. trachomatis, 3 had IgG titers highest against C. pneumoniae, and 1 had undetectable titers for the three chlamydial species. Twenty‐five patients had positive IgG and/or IgM titers to C. trachomatis but negative antigen test results. Serologic testing can increase the sensitivity of detecting C. trachomatis infections. J. Clin. Lab. Anal. 25:305–308, 2011. © 2011 Wiley‐Liss, Inc.
Design and synthesis of some novel quinoline derivatives as anticancer and radiosensitizing agents targeting VEGFR tyrosine kinase
Authors: Ghorab Mostafa M. , Ragab Fatma A. , Heiba Helmy I. , Ghorab Walid M. 
Published: July 2011

AbstractQuinoline derivatives posses many types of biological activities and have been reported to show significant anticancer activity. There is a variety of mechanisms for the anticancer activity and the most distinguished mechanism is the inhibition of vascular epithelial growth factor receptor tyrosine kinase (VEGFRTK). Novel quinoline derivatives 6, 7a, 7b, 7c, 8, 9, 10, 11, 12 and pyrimido[4,5‐b]quinoline derivatives 16, 17, 18, 19, 20 are reported herein. All the newly synthesized compounds were evaluated for their in vitro anticancer activity against human breast cancer cell line (MCF7) in which VEGFR is highly expressed. Compounds 6 and 7 with IC50 values of 8.5 μM and 21.9 μM were the most active compounds and exhibited cytotoxic activities higher than that of the reference drug doxorubicin (IC50= 32.02 μM). The most active compounds 6 and 7 were further evaluated for their ability to enhance the cell killing effect of γ‐radiation. J. Heterocyclic Chem., (2011).
A 1900‐year paleohurricane record from Wassaw Island, Georgia, USA
Authors: Kiage Lawrence M , Deocampo Daniel , Mccloskey Terrence A. , Bianchette Thomas A. , Hursey Melissa 
Published: July 2011

AbstractWe present here the first paleotempestology record from Wassaw Island on the Georgia Bight, located in the south Atlantic coast of the United States. In the historical period, the Georgia Bight has experienced less frequent hits by hurricanes than other locations along the US Gulf and Atlantic coasts. The 1900‐year record inferred from the overwash sand layers from a back‐barrier marsh on Wassaw Island suggests that the island was directly struck by major storms on nine occasions during this interval. The Wassaw Island record shows alternating regimes, with periods of increased activity from >2000 a BP until ∼1100 a BP, and ∼100 a BP until the present, sandwiching a quiet period from ∼1100 to 250 a BP. Storm deposits from the most recent active period are perhaps amalgamated, indicating that site‐specific conditions may result in event undercounting and suggests that the relative thickness of sand layers in the sedimentary record is not always indicative of hurricane strength. Copyright © 2011 John Wiley & Sons, Ltd.
Monolithic media for applications in affinity chromatography
Authors: Sproß Jens , Sinz Andrea 
Published: July 2011

AbstractAffinity chromatography presents a highly versatile analytical tool, which relies on exploiting highly specific interactions between molecules and their ligands. This review covers the most recent literature on the application of monoliths as stationary phases for various affinity‐based chromatographic applications. Different affinity approaches as well as separations using molecularly imprinted monoliths are discussed. Hybrid stationary phases created by embedding of particles or nanoparticles into a monolithic stationary phase are also considered in this review article. The ease of preparation of monoliths and the multitude of functionalization techniques, which have matured during the past years, make monoliths interesting for an increasing number of biochemical and medical applications.
Transparent polyimide nanocomposite films: Thermo‐optical properties, morphology, and gas permeability
Authors: Min Ungki , Kim Jeong‐Cheol , Chang Jin‐Hae 
Published: July 2011

AbstractPoly(amic acid) (PAA) hybrids with organically modified montmorillonite (Cloisite 30B) were synthesized from N,N′‐dimethylacetamide (DMAc) solution. These hybrids were heated at various temperatures, yielding 64‐ to 68‐μm thick films of polyimide (PI)/Cloisite 30B hybrids with various clay contents (0–1.5 wt%). The intercalation of PI chains among the organoclay particles was examined using wide‐angle X‐ray diffraction (XRD) and electron microscopy (TEM) techniques. The thermo‐optical properties were measured by differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and ultraviolet‐visible (UV–vis) spectrometry. The optimum thermal and oxygen barrier properties were observed for the hybrids containing 1.0 wt% Cloisite 30B; these properties were degraded gradually by further increases in the clay content. The presence of only 1.0 wt% Cloisite 30B in a PI hybrid film was found to result in an 83% reduction in the rate of O2 permeability with respect to that of the pure PI film. The PI hybrid films were found to exhibit excellent optical transparency and to be almost colorless. However, the transparency of the hybrid films decreased slightly with increasing organoclay content. POLYM. ENG. SCI., 2011. © 2011 Society of Plastics Engineers
Characterization of well‐defined poly(ethylene glycol) hydrogels prepared by thiol‐ene chemistry
Authors: Yang Ting , Long Hui , Malkoch Michael , Kristofer Gamstedt E. , Berglund Lars , Hult Anders 
Published: July 2011

AbstractConsidering the large number of applications for hydrogels, a better understanding of the relation between molecular structure and mechanical properties for well‐defined hydrogel is essential. A new library has been compiled of poly(ethylene glycol) polymers (PEG) of different length end functionalized with diallyl, dithiol, and dimethacrylate, and crosslinked with complementary trifunctional crosslinkers. In this study, the hydrogels were initially analyzed by FT‐Raman and NMR to study the conversion ratio of the functional groups. The effects of solvent type, solid content concentration, curing time and length of the PEG chains on the final leaching, swelling and tensile properties of the hydrogels were studied. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011
RAFT kinetics revisited: Revival of the RAFT debate
Authors: Junker Tanja 
Published: July 2011

AbstractRecently, two electron spin resonance (ESR)‐based methods for the determination of addition and fragmentation rate coefficients in dithiobenzoate‐mediated reversible addition fragmentation transfer (RAFT) polymerization were introduced, one being based on a spin‐trapping method and the other on single‐laser pulse initiation in conjunction with ESR detection at microsecond time resolution. For the RAFT‐intermediate radical fragmentation rate, coefficient data differing by six orders of magnitude were obtained, which cannot be explained by the usual model dependencies, that is the so‐called cross‐termination versus stable intermediate model. Even under consideration of fast cross‐termination in both cases, the large difference persists. Both the experimental designs are thus critically reviewed to identify potential error sources and to explain the vast difference in the individual results. Both techniques appear to be robust and only small interferences could be identified. Finally, recommendations for the refinement of the individual techniques are given to achieve a consistent kinetic picture of the underpinning reaction equilibria. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011