bluuebunny/crossref_metadata_2025_split huggingface dataset:
UV‐Rearranged PIM‐1 Polymeric Membranes for Advanced Hydrogen Purification and Production
Authors: Li Fu Yun , Xiao Youchang , Ong Yee Kang , Chung Tai‐Shung 
Published: July 2012

AbstractPolymers of intrinsic microporosity (PIM‐1) have been known for their super high permeability but average selectivity for medium‐size gas pairs. They have unimpressive selectivity for H2 and CO2 separation (i.e., α (H2/CO2) = 0.6). For the first time, we have discovered that ultraviolet (UV)‐rearranged polymers of PIM‐1 membranes can be used for H2/CO2 separation with far superior separation performance to others in literatures. The PIM‐1 membrane after UV radiation for 4 hours shows H2 permeability of 452 barrer with H2/CO2 selectivity of 7.3. Experimental data and molecular simulation reveal that the polymer chains of PIM‐1 undergo 1,2‐migration reaction and transform to close‐to‐planar like rearranged structure after UV radiation. As a result, the UV‐irradiated PIM‐1 membrane shows considerable drops in both fractional free volume (FFV) and size of micro‐pores. Positron annihilation lifetime (PAL) results have confirmed the chemical and structural changes, suggesting the FFV and pore size drops are mainly ascribed to the destructed spiro‐carbon centre during UV radiation. Sorption and x‐ray diffractor (XRD) analyses indicate that the impressive H2/CO2 selectivity arises from the significantly enhanced diffusivity selectivity induced by UV radiation, followed by molecular rearrangement, conformation change and chain packing.
Role of autologous hematopoietic stem cell transplantation according to the NPM1/FLT3‐ITD molecular status for cytogenetically normal AML patients: A GOELAMS study
Authors: Guièze Romain , Cornillet‐Lefebvre Pascale , Lioure Bruno , Blanchet Odile , Pigneux Arnaud , Recher Christian , Bonmati Caroline , Fegueux Nathalie , Bulabois Claude‐Eric , Bouscary Didier , Vey Norbert , Delain Martine , Turlure Pascal , Himberlin Chantal , Harousseau Jean‐Luc , Dreyfus Francois , Béné Marie C. , Ifrah Norbert , Chevallier Patrice ,   on behalf of the GOELAMS
Published: July 2012

AbstractThe choice of postremission therapy for acute myeloid leukemia (AML) patients is now based on the blasts' cytogenetic and molecular profile. However, the potential benefit of autologous hematopoietic stem cell transplantation (auto‐HSCT) according to the NPM1/FLT3‐ITD status has been poorly studied in AML patients with a normal karyotype (NK). Therefore, we evaluated the NPM1/FLT3‐ITD molecular status in 135 NK‐AML patients treated by allogeneic HSCT (allo‐HSCT), auto‐HSCT, or chemotherapy as consolidation therapy within the GOELAMS LAM‐2001 trial. In univariate analyzes, 4‐year leukemia‐free survival (LFS) and overall survival (OS) were significantly higher for NPM1+/FLT3‐ITD− patients compared with patients presenting another molecular profile (61 vs. 43% and 72 vs. 48%, P = 0.02 and P = 0.01, respectively). In the NPM1+/FLT3‐ITD− subgroup, there was no benefit for allo‐HSCT or auto‐HSCT vs. chemotherapy (4‐year LFS: 71, 56, and 60%; 4‐year OS: 73, 71, and 60%, respectively; P = NS). For patients with other NPM1/FLT3‐ITD molecular profiles, allo‐HSCT was found to be the best consolidation therapy, whereas auto‐HSCT was associated with a better outcome when compared with chemotherapy (allo‐HSCT‐, auto‐HSCT‐, and chemotherapy‐related 4‐year LFS: 68, 44, and 36%, P = 0.004; 4‐year OS: 68, 52, and 29%, respectively, P = 0.02). Our study indicates that allo‐HSCT and auto‐HSCT provide similar outcomes compared with chemotherapy as consolidation for NPM1+/FLT3‐ITD− NK‐AML patients. For NK‐AML patients with an adverse molecular profile, auto‐HSCT could represent an alternative therapeutic approach when no human leukocyte antigen–matched allogeneic donor is available. Am. J. Hematol. 2012. © 2012 Wiley Periodicals, Inc.
N‐methyl‐D‐aspartate receptor antibodies in herpes simplex encephalitis
Authors: Prüss Harald , Finke Carsten , Höltje Markus , Hofmann Joerg , Klingbeil Christine , Probst Christian , Borowski Kathrin , Ahnert‐Hilger Gudrun , Harms Lutz , Schwab Jan M. , Ploner Christoph J. , Komorowski Lars , Stoecker Winfried , Dalmau Josep , Wandinger Klaus‐Peter 
Published: July 2012

AbstractObjective:To determine the presence and kinetics of antibodies against synaptic proteins in patients with herpes simplex virus encephalitis (HSE).Methods:Retrospective analysis of 44 patients with polymerase chain reaction‐proven HSE for the presence of a large panel of onconeuronal and synaptic receptor antibodies. The effect of patients' serum was studied in cultures of primary mouse hippocampal neurons.Results:N‐Methyl‐D‐aspartate receptor (NMDAR) antibodies of the immunoglobulin (Ig) subtypes IgA, IgG, or IgM were detected in 13 of 44 patients (30%) in the course of HSE, suggesting secondary autoimmune mechanisms. NMDAR antibodies were often present at hospital admission, but in some patients developed after the first week of HSE. Antibody‐positive sera resulted in downregulation of synaptic marker proteins in hippocampal neurons.Interpretation:Some patients with HSE develop IgA, IgG, or IgM autoantibodies against NMDAR. Sera from these patients alter the density of neuronal synaptic markers, suggesting a potential pathogenic disease‐modifying effect. These findings have implications for the understanding of autoimmunity in infectious diseases, and prospective studies should reveal whether the subgroup of patients with HSE and NMDAR antibodies may benefit from immunotherapy. ANN NEUROL 2012;72:902–911
Real‐time diagnosing polymer processing in injection molding using ultrasound
Authors: Zhao Lijuan , Lai Yu , Pei Chen , Jen Cheng‐Kui , Wu Kuo‐Ding 
Published: July 2012

AbstractUltrasonic diagnosing technique with a new high‐temperature ultrasonic transducer is developed to real‐time diagnose polymer processing and its morphology changes in injection molding processing. Compared with the previous researches, the new technique can provide more and accurate information. In this study, ultrasound diagnosis shows that longitudinal wave can real‐time characterize the data of the injection process and polymer morphology changes, including melt flow arrival time, the part ejection time, filling and packing stages, polymer solidification process, and the morphology changes during polymer crystallization. Shear waves can real‐time diagnose Young's and shear storage modulus, anisotropy property of polymer in injection molding. During our research, real‐time ultrasonic diagnosis shows that the storage modulus along the vertical direction is larger than that of the parallel to the melt flow direction under our setup injection conditions. Scanning electron microscopy and dynamic mechanical analysis measurements present that it is because the crystalline lamellas of HDPE are parallel arrangement and grow in a vertical to melt flow direction owing to injection shear force under a certain injection conditions. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Development and preparation of high‐performance thermoplastic vulcanizates based on blends of natural rubber and thermoplastic polyurethanes
Authors: Kalkornsurapranee Ekwipoo , Nakason Charoen , Kummerlöwe Claudia , Vennemann Norbert 
Published: July 2012

AbstractNatural rubber (NR)/thermoplastic polyurethane (TPU) blends were prepared with a dynamic vulcanization technique. The main objective was to prepare high‐performance thermoplastic vulcanizates (TPVs) based on natural rubber [thermoplastic natural rubber (TPNR)] with low hardness values and improved thermal and mechanical properties. In this study, the preparation of dynamically cured NR/TPU blends was carried out systematically, and the final properties were compared to those of typical commercial TPVs. The influence of sulfur‐cured systems, accelerator types, process parameters, and rubber types were investigated. The developed NR/TPU‐based TPNRs showed better tensile strengths and thermal properties than commercial TPVs with similar hardnesses. Moreover, the epoxidized natural rubber (ENR)/TPU‐based TPNRs exhibited a low compression set. The incorporation of NR into the TPU reduced the Shore A hardness to about 60 in comparison to a value of about 85 for the neat TPU; the blends also showed improved oil resistance (oil swelling = 12%) in comparison to commercial TPVs with similar hardnesses. The experimental results indicate that high‐performance TPNRs with low hardness values could be prepared with the proper conditions developed in this study. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
The electrospun polyamide 6 nanofiber membranes used as high efficiency filter materials: Filtration potential, thermal treatment, and their continuous production
Authors: Guibo Yin , Qing Zhao , Yahong Zhao , Yin Yuan , Yumin Yang 
Published: July 2012

AbstractIn this article, polyamide (PA) 6 was dissolved in 98 wt % formic acids with a concentration of 13 wt %, and then the electrospun PA 6 nanofiber membranes were prepared. The filtration potential of the nanofiber membranes were firstly analyzed based on the scanning electron microscope images and pore size measurements, and then the effects of thermal treatment on the dimensional stability were studied. Subsequently, the continuous production process based on the above experiments and the deposition areas of the nanofibers on the collecting meshes was designed. Finally, the mechanical properties and the filtration performances of the electrospun PA 6 nanofiber membranes were assessed. The results showed the membranes with a thickness of 71 μm (electrospinning for 15 min) had good filtration potential for the microparticles with 0.3 μm diameter. After being treated by tension and relaxation heat setting, the membranes demonstrated excellent dimensional stability, the breaking tenacity, and the elongation reached 4.71 ± 1.66 MPa and (69.97 ± 6.56)%, respectively. The permeability decreased with the increase of the membranes thickness. However, the permeability of membranes with (72.9 ± 1.04) μm thickness still maintained 516 L/m2 s, and the actual filtration efficiency of 0.3 μm particles reached 99.98%. The above results showed that the membranes fabricated by the continuous production process could meet the demands of high efficiency filtration and provide a reference for the industrial use of electrospun nanofiber membranes. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
An investigation into surface modification of polyethylene films for hydrophilicity enhancement by catalytic ozonation
Authors: Mastan Erlita , Wu Jiangning , Doan Huu 
Published: July 2012

AbstractFor a conventional polyethylene film, ozonation was conducted to increase its surface hydrophilicity. Copper sulfate was found effective as a catalyst in aqueous ozonation to increase the generation of surface peroxides significantly, which is the essential step for hydrophilicity enhancement. The percentage increase in peroxide generation could range from 41% to 58% by aqueous catalytic ozonation over its noncatalyzed gaseous counterpart. The optimum concentration of copper sulfate was found to be 0.05 g/L. After adding the catalyst, the length of ozonation could be significantly reduced so that the bulk mechanical strength of the films, represented by tensile strength, could be well preserved after ozonation. A novel kinetic model for the aqueous ozonation was proposed. This model was developed by combing the reaction mechanism of gaseous ozonation of polyethylene films with that of ozone self‐decomposition in water. The peroxide generation also increased with the applied ozone dose. The surface morphology and contact angle of the film were all examined before and after ozonation. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Poly(vinylidene fluoride)/halloysite nanotubes nanocomposites: The structures, properties, and tensile fracture behaviors
Authors: Tang Xue‐Gang , Hou Meng , Zou Jin , Truss Rowan 
Published: July 2012

AbstractPoly(vinylidene fluoride)/halloysite nanotubes (HNTs) nanocomposites with good dispersion were prepared. The effect of HNTs on the crystalline structure, dynamic rheological properties, dynamic mechanical properties, and tensile properties was investigated. Results showed that at low concentration, the effect of HNTs on the modulus was minor. However, at high concentration, improvement of the modulus can be observed. The tensile fracture behaviors were investigated, which showed that the addition of HNTs changed the fracture mode. When the concentration of HNTs was 20 phr, a fracture mode change from microductility/brittle to brittle/fibrillation occurred. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Evaluation of a nanocomposite hydrogel for water shut‐off in enhanced oil recovery applications: Design, synthesis, and characterization
Authors: Tongwa Paul , Nygaard Runar , Bai Baojun 
Published: July 2012

AbstractThis work involves the synthesis of a nanocomposite hydrogel from just polymer and clay without the use of conventional organic crosslinkers. Conventional hydrogel design usually involves a multicomponent reaction that incorporates monomer (or polymer), initiator, and an organic crosslinker. However, because of the many limitations, setbacks, and inconsistencies involved with organic crosslinkers, authors herein present a nanocomposite hydrogel that incorporates polymer and clay only. It was found that these hydrogels show surprising mechanical toughness, tensile moduli, and tensile strengths. Study of gel behavior reveal physical interaction between polymer and clay, due in part to adsorption of polymer chains onto clay surface and ionic interactions between anionic carboxylate groups of polymer chains and positive clay surface. X‐ray diffraction patterns and Scanning Electron Microscopy revealed the formation of intercalated and exfoliated clay morphology. Increase in clay concentration and gel strength had a direct proportionality. The effect of clay concentration on hydrogel decomposition temperature was also reported by thermogravimetric analysis. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Novel epoxidized hyperbranched poly(phenylene oxide): Synthesis and application as a modifier for diglycidyl ether of bisphenol A
Authors: Lv Jianyong , Meng Yan , He Lifan , Qiu Teng , Li Xiaoyu , Wang Haiqiao 
Published: July 2012

AbstractA novel epoxidized hyperbranched poly(phenylene oxide) (EHPPO) is designed and synthesized successfully. The structure of EHPPO is characterized by Fourier transform infrared spectra‐ and quantitative 13C nuclear magnetic resonance spectrum. The synthesized EHPPO is added into diglycidyl ether of bisphenol A as a modifier in different ratios to form hybrids and cured by an anhydride curing agent. Effects of EHPPO addition on the properties of the cured hybrids are investigated. Thermal mechanical analysis results suggest that addition of EHPPO can increase the free volume of the cured hybrid materials. Dynamic mechanical analysis characterizations show that the crosslinking density increases with the increase in EHPPO content. Furthermore, addition of EHPPO results in an improvement in thermal and mechanical properties. The toughening mechanism is also discussed. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
T lymphocytes and muscle condition act like seeds and soil in a murine polymyositis model
Authors: Okiyama Naoko , Sugihara Takahiko , Oida Takatoku , Ohata Junko , Yokozeki Hiroo , Miyasaka Nobuyuki , Kohsaka Hitoshi 
Published: July 2012

AbstractObjectiveIt has been reported that polymyositis (PM) is driven by CD8+ cytotoxic T lymphocytes. The C protein–induced myositis (CIM) model we have established is similar to PM in pathology except that it undergoes spontaneous remission. We undertook the present study to delineate the roles of innate and acquired immunity in myositis.MethodsC57BL/6 mice were immunized with recombinant C protein fragments together with Freund's complete adjuvant (CFA) and Toll‐like receptor (TLR) ligands at hind leg footpads and tail bases. CIM mediated by adoptive transfer of T cells to naive mice was treated with cytokine antagonists.ResultsSecond immunization with C protein fragments revealed no induction of tolerance. Injection of CFA and TLR ligands at the hind leg footpads reinduced myositis in the same legs. Interestingly, initial myositis was observed only in the CFA‐treated forelegs. Transfer of C protein fragment–specific T cells from mice with CIM induced myositis in CFA‐ and TLR ligand–treated legs of recipient mice. CFA treatment resulted in the recruitment of macrophages producing inflammatory cytokines. Induction of myositis was inhibited by blocking interleukin‐1 receptor or tumor necrosis factor α.ConclusionMyositis development requires activation of autoaggressive T cells and conditioning of muscle tissue. CIM regression is due to attenuation of local CFA‐induced immune activation. These results are in accordance with a “seed and soil” model of disease development and might offer clues to decipher clinical aspects of PM.
Chlorine abstraction reaction from chloropentafluorobenzene by the CF3 radical
Authors: Nieto J.D. , Herrera O.S. , Lane S.I. , Oexler E.V. 
Published: July 2012

AbstractThe temperature dependence of the rate constant for the gas phase abstraction reaction of chlorine by CF3 from chloropentafluorobenzene was determined in the temperature range 573‐653 K by the competitive method using the recombination of CF3 radicals as reference reaction. Taking a value of 1.53·1013 cm3 mol−1 s−1 for the recombination rate constant, kcl=(6.76±1.26) 1012 exp [−(6339±103) K/T) cm3 mol−1 s−1.The Arrhenius parameters were calculated by the Bond Strength‐Bond Length method (BSBL) and good agreement was obtained with the experimental values.
Solvent influence on the electroacoustic excitation of sound in electrolytes
Authors: Tankovsky N. , Bärner K. 
Published: July 2012

AbstractThe influence of different mixed solvents on the efficiency of sound excitation in electrolytes by means of an a.c. electric field is studied experimentally. The ability of the solvent molecules to associate in structures and to solvatate the ions proves to be important for the electroacoustic mechanisms and their dynamical characteristics.
Fabrication of gelatin nanofibrous scaffolds using ethanol/phosphate buffer saline as a benign solvent
Authors: Zha Zhengbao , Teng Weibing , Markle Valerie , Dai Zhifei , Wu Xiaoyi 
Published: July 2012

AbstractElectrospinning of natural polymer nanofibers useful for biomedical applications often requires the use of cytotoxic organic solvents. In this study, gelatin nanofibers are electrospun from phosphate buffer saline/ethanol binary mixtures as a benign solvent at ambient temperature. The influences of ionic strength, ethanol concentration, and gelatin concentration on the electrospinnability of gelatin solutions and the fiber microarchitectures are analyzed. The electrospun scaffolds retain their morphologies during vapor‐phase crosslinking with glutaraldehyde in ethanol and the subsequent removal of salts contained in the nanofibers via water rinsing. When fully hydrated, the mechanically preconditioned scaffolds display a Young's modulus of 25.5 ± 5.3 kPa, tensile strength of 55.5 ± 13.9 kPa, deformability of 160 ± 15%, and resilience of 89.9 ± 1.8%. When cultured on the gelatin scaffolds, 3T3 fibroblasts displayed spindle‐like morphology, similar to the cell's normal morphology in a 3D extracellular matrix. © 2012 Wiley Periodicals, Inc. Biopolymers 97:1026–1036, 2012.
Structure–activity studies of lGnRH‐III through rational amino acid substitution and NMR conformational studies
Authors: Pappa Eleni V. , Zompra Aikaterini A. , Diamantopoulou Zoi , Spyranti Zinovia , Pairas George , Lamari Fotini N. , Katsoris Panagiotis , Spyroulias George A. , Cordopatis Paul 
Published: July 2012

AbstractLamprey gonadotropin‐releasing hormone type III (lGnRH‐III) is an isoform of GnRH isolated from the sea lamprey (Petromyzon marinus) with negligible endocrine activity in mammalian systems. Data concerning the superior direct anticancer activity of lGnRH‐III have been published, raising questions on the structure–activity relationship. We synthesized 21 lGnRH‐III analogs with rational amino acid substitutions and studied their effect on PC3 and LNCaP prostate cancer cell proliferation. Our results question the importance of the acidic charge of Asp6 for the antiproliferative activity and indicate the significance of the stereochemistry of Trp in positions 3 and 7. Furthermore, conjugation of an acetyl‐group to the side chain of Lys8 or side chain cyclization of amino acids 1‐8 increased the antiproliferative activity of lGnRH‐III demonstrating that the proposed salt bridge between Asp6 and Lys8 is not crucial. Conformational studies of lGnRH‐III were performed through NMR spectroscopy, and the solution structure of GnRH‐I was solved. In solution, lGnRH‐III adopts an extended backbone conformation in contrast to the well‐defined β‐turn conformation of GnRH‐I. © 2012 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 98: 525–534, 2012.
The protein arginine deiminases: Structure, function, inhibition, and disease
Authors: Bicker Kevin L. , Thompson Paul R. 
Published: July 2012

AbstractThe post‐translational modification of histones has significant effects on overall chromatin function. One such modification is citrullination, which is catalyzed by the protein arginine deiminases (PADs), a unique family of enzymes that catalyzes the hydrolysis of peptidyl‐arginine to form peptidyl‐citrulline on histones, fibrinogen, and other biologically relevant proteins. Overexpression and/or increased PAD activity is observed in several diseases, including rheumatoid arthritis, Alzheimer's disease, multiple sclerosis, lupus, Parkinson's disease, and cancer. This review discusses the important structural and mechanistic characteristics of the PADs, as well as recent investigations into the role of the PADs in increasing disease severity in RA and colitis and the importance of PAD activity in mediating neutrophil extracellular trap formation through chromatin decondensation. Lastly, efforts to develop PAD inhibitors with excellent potency, selectivity and in vivo efficacy are discussed, highlighting the most promising inhibitors. © 2012 Wiley Periodicals, Inc. Biopolymers 99: 155–163, 2013.
Quantitative analysis of enzymatic fractionation of multiple substrate mixtures
Authors: Kaki Shiva Shanker , Adlercreutz Patrick 
Published: July 2012

AbstractThe enzymatic conversion of mixtures of multiple substrates was studied quantitatively, based on established methodology used for the enzymatic kinetic resolution of racemic mixtures, involving the use of competitive factors: ratios of specificity constants (kcat/KM) of substrate pairs. The competitive factors of the substrates were defined in relation to a reference substrate. These competitive factors were used to predict the composition of the reaction mixture as a function of the degree of conversion of the reaction. The methodology was evaluated using three different lipases to hydrolyze a model mixture of four fatty acid methyl esters and for the esterification of a mixture of the same fatty acids in free form with ethanol. In most cases, the competitive factors determined from the initial phase of the reactions predicted the product composition during the rest of the reaction very well. The slowest reacting fatty acid was erucic acid (both in free form and as methyl ester), which was thus enriched in the remaining substrate fraction, while the other fatty acids: lauric acid, palmitic acid and oleic acid were converted faster. Simulations of the compositions of reaction mixtures with different values of the competitive factors were carried out to provide an overview of what could be achieved using enzymatic enrichment. Possible applications include reactions involving homologous substrates and mixtures of multiple isomers. The analysis presented provides guidelines that can be useful in the screening and development of enzymes for enzymatic enrichment applications. Biotechnol. Bioeng. 2013; 110: 78–86. © 2012 Wiley Periodicals, Inc.