bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discovery of biomarkers in the psoriasis through machine learning and dynamic immune infiltration in three types of skin lesions
Authors: Zhou Xiao , Zhou Han , Luo Xin , Wu Rui-Fang 
Published: May 2024

IntroductionPsoriasis is a chronic skin disease characterized by unique scaling plaques. However, during the acute phase, psoriatic lesions exhibit eczematous changes, making them difficult to distinguish from atopic dermatitis, which poses challenges for the selection of biological agents. This study aimed to identify potential diagnostic genes in psoriatic lesions and investigate their clinical significance.MethodsGSE182740 datasets from the GEO database were analyzed for differential analysis; machine learning algorithms (SVM-RFE and LASSO regression models) are used to screen for diagnostic markers; CIBERSORTx is used to determine the dynamic changes of 22 different immune cell components in normal skin lesions, psoriatic non-lesional skin, and psoriatic lesional skin, as well as the expression of the diagnostic genes in 10 major immune cells, and real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemistry are used to validate results.ResultsWe obtained 580 differentially expressed genes (DEGs) in the skin lesion and non-lesion of psoriasis patients, 813 DEGs in mixed patients between non-lesions and lesions, and 96 DEGs in the skin lesion and non-lesion of atopic dermatitis, respectively. Then 144 specific DEGs in psoriasis via a Veen diagram were identified. Ultimately, UGGT1, CCNE1, MMP9 and ARHGEF28 are identified for potential diagnostic genes from these 144 specific DEGs. The value of the selected diagnostic genes was verified by receiver operating characteristic (ROC) curves with expanded samples. The the area under the ROC curve (AUC) exceeded 0.7 for the four diagnosis genes. RT-qPCR results showed that compared to normal human epidermis, the expression of UGGT1, CCNE1, and MMP9 was significantly increased in patients with psoriasis, while ARHGEF28 expression was significantly decreased. Notably, the results of CIBERSORTx showed that CCNE1 was highly expressed in CD4+ T cells and neutrophils, ARHGEF28 was also expressed in mast cells. Additionally, CCNE1 was strongly correlated with IL-17/CXCL8/9/10 and CCL20. Immunohistochemical results showed increased nuclear expression of CCNE1 in psoriatic epidermal cells relative to normal.ConclusionBased on the performance of the four genes in ROC curves and their expression in immune cells from patients with psoriasis, we suggest that CCNE1 possess higher diagnostic value.
Case report: Donor-derived CLL-1 chimeric antigen receptor T-cell therapy for relapsed/refractory acute myeloid leukemia bridging to allogeneic hematopoietic stem cell transplantation after remission
Authors: Miao Xiaojuan , Shuai Yanrong , Han Ying , Zhang Nan , Liu Yilan , Yao Hao , Wang Xiao , He Guangcui , Chen Dan , Fan Fangyi , Chang Alex H. , Su Yi , Yi Hai 
Published: May 2024

BackgroundExplore the efficacy and safety of donor-derived CLL-1 chimeric antigen receptor T-cell therapy (CAR-T) for relapsed/refractory acute myeloid leukemia (R/R AML) bridging to allogeneic hematopoietic stem cell transplantation (allo-HSCT) after remission.Case presentationAn adult R/R AML patient received an infusion of donor-derived CLL-1 CAR-T cells, and the conditioning regimen bridging to allo-HSCT was started immediately after remission on day 11 after CAR-T therapy upon transplantation. Then, routine post-HSCT monitoring of blood counts, bone marrow (BM) morphology, flow cytometry, graft-versus-host disease (GVHD) manifestations, and chimerism status were performed.ResultAfter CAR-T therapy, cytokine release syndrome was grade 1. On day 11 after CAR-T therapy, the BM morphology reached complete remission (CR), and the conditioning regimen bridging to allo-HSCT started. Leukocyte engraftment, complete donor chimerism, and platelet engraftment were observed on days +18, +23, and +26 post-allo-HSCT, respectively. The BM morphology showed CR and flow cytometry turned negative on day +23. The patient is currently at 4 months post-allo-HSCT with BM morphology CR, negative flow cytometry, complete donor chimerism, and no extramedullary relapse/GVHD.ConclusionDonor-derived CLL-1 CAR-T is an effective and safe therapy for R/R AML, and immediate bridging to allo-HSCT after remission may better improve the long-term prognosis of R/R AML.
Geriatric nutritional risk index and controller nutritional status score before metastatic first-line chemotherapy predict survival in patients over 70 years of age with metastatic bladder cancer
Authors: Balçık Onur Yazdan , Demir Bilgin , Ilhan Yusuf , Akagündüz Baran 
Published: May 2024

IntroductionSeveral prognostic factors have been identified in patients with metastatic bladder cancer (BC). As it is known, older adult patients are prone to nutritional deficiency. The knowledge about nutrition and impact on survival in older patients with metastatic bladder cancer is missing. It is necessary to specifically examine this population. Because timely interventions can make a positive impact on this patients population. This retrospective study aimed to evaluate the prognostic effect of the Geriatric Nutritional Risk Index (GNRI), Controller Nutritional Status (CONUT) score and Prognostic Nutritional Index (PNI) before first-line chemotherapy in the metastatic stage in patients with metastatic bladder cancer over 70.Participants and methodsPatients over 70 with pathologically confirmed denovo metastatic or recurrent metastatic bladder cancer were included in the study. Patients with infections diagnosed at the time of diagnosis, autoimmune diseases or history of steroid use were excluded. Since our population consists of a specific age group with a specific cancer, we found a new cut-off value by performing ROC analysis to ensure optimal sensitivity and specificity in terms of progression. Low GNRI value was related with poor nutritional status. Low PNI value was related with poor nutritional status and high CONUT score was related with poor nutritional status. Factors predicting overall survival (OS) and Progression-Free Survival (PFS) were assessed using both univariate and multivariate Cox proportional hazards analyses.Results106 patients were included in the study and the average age was 75.5 years. In the GNRI-Low group, PFS was significantly shorter than that in the GNRI-High group [HR (95% CI) = 57.1 (12.8–255.5), (p &lt; 0.001)]. Among those with a low-CONUT score, PFS was found to be longer than that in the high-CONUT group [HR (95% CI) = 1.7 (1.0–3.0), (p = 0.039)]. The median PFS of the PNI-Low group wasn’t significantly shorter than that of the PNI-High group [HR (95% CI) = 1.8 (0.5–6.2), (p = 0.359)].ConclusionOur study suggests that the GNRI and CONUT scores are useful for predicting survival in patients over 70 years of age with BC.
Repeat biopsy versus initial biopsy in terms of complication risk factors and clinical outcomes for patients with non-small cell lung cancer: a comparative study of 113 CT-guided needle biopsy of lung lesions
Authors: Wang Yangyang , Zhang Yongyuan , Ren Nana , Li Fangting , Lu Lin , Zhao Xin , Zhou Zhigang , Gao Mengyu , Wang Meng 
Published: May 2024

ObjectivesThe safety and feasibility of repeat biopsy after systemic treatment for non-small cell lung cancer have received extensive attention in recent years. The purpose of this research was to compare complication rates between initial biopsy and rebiopsy in non-small cell lung cancer patients with progressive disease and to assess complication risk factors and clinical results after rebiopsy.MethodsThe study included 113 patients initially diagnosed with non-small cell lung cancer who underwent lung biopsy at initial biopsy and rebiopsy after progression while on epidermal growth factor receptor tyrosine kinase inhibitors (EGFR-TKIs) and/or chemotherapy from January 2018 to December 2021. We compared the incidence of complications between the initial biopsy and rebiopsy and analyzed the predictors factors that influenced complications in patients who underwent rebiopsy.ResultsThe successful rate of rebiopsy was 88.5% (100/113). With the exception of two cases where lung adenocarcinoma changed into small cell lung cancer with gefitinib treatment, 98 individuals retained their initial pathological type. The secondary EGFR T790M mutation accounts for 55.6% of acquired resistance. The total number of patients with complications in initial biopsy was 25 (22.1%) and 37 (32.7%) in the rebiopsy. The incidence of pulmonary hemorrhage increased from 7.1% at the initial biopsy to 10.6% at rebiopsy, while the incidence of pneumothorax increased from 14.2% to 20.4%. Compared with the initial biopsy, the incidence of overall complications, parenchymal hemorrhage, and pneumothorax increased by 10.6%, 3.5%, and 6.2%, respectively. In all four evaluations (pneumorrhagia, pneumothorax, pleural reaction, and overall complication), there were no significant differences between the rebiopsy and initial biopsy (all p &gt; 0.05). The multivariate logistic regression analysis suggested that male sex (odds ratio [OR] = 5.064, p = 0.001), tumor size ≤ 2 cm (OR = 3.367, p = 0.013), EGFR-TKIs with chemotherapy (OR = 3.633, p =0.023), and transfissural approach (OR = 7.583, p = 0.026) were independent risk factors for overall complication after rebiopsy.ConclusionCompared with the initial biopsy, the complication rates displayed a slight, but not significant, elevation in rebiopsy. Male sex, tumor size ≤ 2 cm, transfissural approach, and EGFR-TKIs combined with chemotherapy were independent risk factors for rebiopsy complications.
The effect of physical activity on anxiety symptoms among children and adolescents with mental health disorders: a research brief
Authors: Anker Ella Aase , Bøe Sture Svanhild E. , Hystad Sigurd William , Kodal Arne 
Published: May 2024

BackgroundAnxiety is prevalent among children and adolescents (termed youths), and leads to reduced quality of life, disability, loss of education and reduced life-span. Physical activity has shown promising effects on symptoms of anxiety in adult populations, and an increasing amount of research has also demonstrated some effect in youth. However, physical activity is not widely used in youth mental health care, and research is very limited.Methods/designThis single arm, pre-post study explores the effect of a manualized physical activity-based 14-session intervention termed Confident, Active and Happy Youth. Participants are youth attending specialized mental health care (N=51, M age = 13.4, SD = 2.2). Changes in anxiety symptoms are examined using mixed models with residual maximum likelihood (REML). The potential effect of anxiety subtype differences, participant age, comorbidity, and time spent in out-patient care are explored.ResultsYouths did not report any effect on anxiety symptoms after participation in CAHY, however, their parents report a significant reduction in youth’s anxiety symptoms in general (b = -0.11, 95%, CI: -0.21 to -0.01) and specifically for social phobia (b = -0.19, 95% CI: -0.35 to -0.03). Age and comorbidity showed no significant effect on anxiety symptoms post treatment. Prior treatment time in youth mental health care demonstrated inconclusive results.ConclusionThe study finds initial evidence of symptomatic change in a clinical population of youth’s receiving a physical activity-based intervention. Our research provides preliminary support for physical activity as a supplementary treatment method for mental health disorders among youths.Clinical Trial Registrationclinicaltrials.gov, NCT05049759
Serum levels of neurotensin, pannexin-1, and sestrin-2 and the correlations with sleep quality or/and cognitive function in the patients with chronic insomnia disorder
Authors: Su Ai-Xi , Ma Zi-Jie , Li Zong-Yin , Li Xue-Yan , Xia Lan , Ge Yi-Jun , Chen Gui-Hai 
Published: May 2024

ObjectivesTo examine serum concentrations of neurotensin, pannexin-1 and sestrin-2, and their correlations with subjective and objective sleep quality and cognitive function in the patients with chronic insomnia disorder (CID).MethodsSixty-five CID patients were enrolled continuously and fifty-six good sleepers in the same period were served as healthy controls (HCs). Serum levels of neurotensin, pannexin-1 and sestrin-2 were measured by enzyme-linked immunosorbent assays. Sleep quality was assessed with the Pittsburgh Sleep Quality Index (PSQI) and polysomnography, and mood was evaluated by 17-item Hamilton Depression Rating Scale. General cognitive function was assessed with the Chinese-Beijing Version of Montreal Cognitive Assessment and spatial memory was evaluated by Blue Velvet Arena Test (BVAT).ResultsRelative to the HCs, the CID sufferers had higher levels of neurotensin (t=5.210, p&lt;0.001) and pannexin-1 (Z=−4.169, p&lt;0.001), and lower level of sestrin-2 (Z=−2.438, p=0.015). In terms of objective sleep measures, pannexin-1 was positively associated with total sleep time (r=0.562, p=0.002) and sleep efficiency (r=0.588, p=0.001), and negatively with wake time after sleep onset (r=−0.590, p=0.001) and wake time (r=−0.590, p=0.001); sestrin-2 was positively associated with percentage of rapid eye movement sleep (r=0.442, p=0.016) and negatively with non-rapid eye movement sleep stage 2 in the percentage (r=−0.394, p=0.034). Adjusted for sex, age and HAMD, pannexin-1 was still associated with the above objective sleep measures, but sestrin-2 was only negatively with wake time (r=−0.446, p=0.022). However, these biomarkers showed no significant correlations with subjective sleep quality (PSQI score). Serum concentrations of neurotensin and pannexin-1 were positively associated with the mean erroneous distance in the BVAT. Adjusted for sex, age and depression, neurotensin was negatively associated with MoCA score (r=−0.257, p=0.044), pannexin-1 was positively associated with the mean erroneous distance in the BVAT (r=0.270, p=0.033).ConclusionsThe CID patients had increased neurotensin and pannexin-1 and decreased sestrin-2 in the serum levels, indicating neuron dysfunction, which could be related to poor sleep quality and cognitive dysfunction measured objectively.
“Swipe & slice”: decoding digital struggles with non-suicidal self-injuries among youngsters
Authors: Orsolini Laura , Reina Salvatore , Longo Giulio , Volpe Umberto 
Published: May 2024

IntroductionNonsuicidal-self-injury (NSSI)-related content recently emerged on social networking sites (SNS), despite its relationship with NSSI conducts is still unclear.MethodsHence, the current population-based cross-sectional study investigated the interplay between SNS use, NSSI content engagement, risky social media challenges (RSMCs), in a sample of 404 young adults (aged 18–24), focusing on the influence of problematic social media use (PSMU) and fear of missing out (FoMO).ResultsAround 51.5% of the samplewas engaged inNSSI-related contents on SNS, being mostly females (p&lt;0.001), younger (p=0.005), transgender people and nonbinary people (p=0.030) and those who displayed higher PSMU (p&lt;0.001) or FoMO (p=0.031). Around 66.2% of the sample currently practice NSSIs, predominantly among females (p&lt;0.001), those using BeReal (p=0.012), actively looking for NSSIrelated contents on SNS (p&lt;0.001) to be part of a group (p=0.0025) or learn how to practice NSSI (p=0.025). PSMU (p&lt;0.001) and FoMO (p&lt;0.001) emerged as significant predictors of NSSI content engagement, particularly among active seekers. NSSI conducts were significantly predicted by FoMO (p&lt;0.001) and Snapchat (p=0.044), while negatively predicted by male sex (p&lt;0.001), higher educational level (p=0.019) and age at which NSSI-related contents were firstly looked for (p=0.028).DiscussionThese findings underline the need to implement preventivepolicies and targeted interventions to monitor NSSI-related contents on SNS, the impact of PSMU and FoMO on NSSI, particularly among youngsters.
Resource endowment and sustainable soil fertility management strategies in maize farming systems in northern Benin
Authors: Tovihoudji Pierre G. , Sossa Elvire Line , Egah Janvier , Agbangba Emile C. , Akponikpè P. B. Irenikatché , Yabi Jacob A. 
Published: May 2024

IntroductionManaging soil fertility remains one of the major concerns of smallholder farmers in Sub-Saharan Africa (SSA). In order to understand the adoption potential of recommended practices to smallholders, this study aimed to characterize the resource availability, allocation and use patterns, and the soil fertility management practices in the maize farming system in northern Benin.MethodsA survey was conducted with 262 households randomly selected across three municipalities from three agro-ecological zones. Focus groups discussions and individual interviews were conducted with the household heads using an interview guide and a structured questionnaire respectively. The main data collected included (a) the socio-economic characteristics of households, (b) the main farm type and their characteristics and (c) the current soil fertility management strategies and the rationale behind.Results and discussionThe main soil fertility practices included the use of mineral fertilizer, manure, and crop residues application to plots. As a general trend, the rates of fertilizers applied by farmers [on average 132 ± 8.5 and 59 ± 5.8 kg ha−1 of Nitrogen-Phosphorus-Potassium (NPK) and urea] were below those recommended or required to ensure optimal maize yields. Five farm types corresponding to different resource endowments or wealth classes were identified. The scarce resource farms owned by the majority of poorer farmers use only small amounts of manure and fertilizer in their fields. Qualitative analysis indicated that farms have different constraints and opportunities to adopt newly proposed soil fertility management recommendations.ConclusionExtension services and decision makers must target specific interventions for appropriation and sustainability of technologies to resource mostly the smallholder farmers.
The clinical application of V-Y advanced flap pedicled with freestyle perforator flap for repairing small range defects in the anterior knee region
Authors: Qian Da , Jin Lijie , Huang Guoxin , Dai Ping , Li Dong , Lu Hui , Xu Ming , Wang Ke , Zhong Xian , Xu Xiaochen , Zhang Jianchao , Yu Bin 
Published: May 2024

IntroductionThis study aims to investigate the clinical efficacy of V-Y advanced flap pedicled with freestyle perforator flap for repairing small range defects in the anterior knee region.Methods8 patients with skin and soft tissue defect/necrosis in the anterior knee area admitted to the Changshu No.1 People's Hospital from January 2021 to January 2022 were selected, with a defect range of 4 cm × 3 cm–9 cm × 6 cm, designed a V-Y advanced flap pedicled with freestyle perforator flap to repair the wound in the anterior knee area. Adjust the size and position of the flap according to the number and position of perforating branches found during the surgery, with a cutting area of 6 cm × 5 cm–14 cm × 10 cm and the supply area was directly pulled and sutured.Results4 patients were repaired by flaps pedicled with 2 perforating branches, 2 patients were repaired by flaps pedicled with 1 perforating branch and 2 patients were repaired by flaps pedicled with 3 perforating branches. 4 patients were repaired by flaps pedicled with 2 perforating branches, 2 patients were repaired by flaps pedicled with 1 perforating branch and 2 patients were repaired by flaps pedicled with 3 perforating branches. All flaps survived and following up for 6-15 months, the blood supply, appearance, and color of the flap were satisfactory, and the functions of knee joint flexion and extension were well preserved.DiscussionThe V-Y advancement flap pedicled with freestyle perforator flap has the advantages of reliable blood supply, simple surgical operation, texture and thickness similar to the skin of the anterior knee area, and direct suture of the donor area. It is a perforator flap with good repair effect for small scale defects in the anterior knee area.
Impact of the War in Ukraine on the Ability of Children to Recognize Basic Emotions
Authors: Loshenko Oleksandra , Palíšek Petr , Straka Ondřej , Jabůrek Michal , Portešová Šárka , Ševčíková Anna 
Published: May 2024

ObjectivesThis study assessed emotion recognition skills in school-age children in wartime conditions in Ukraine.MethodsAn online survey based on the concept of basic emotions was administrated to a sample of 419 schoolchildren from Ukraine and a control group of 310 schoolchildren from the Czech Republic, aged 8 to 12.ResultsThere is no difference in judging the intensity of anger and fear by Ukrainian children, compared with the control group. There is no evidence that the emotions of anger, fear, and sadness were better recognized in the Ukrainian group. Children from Ukraine were better at recognizing positive emotions than Czech children.ConclusionIncreased risks of threats and wartime experience do not impair the accuracy of identification of emotions like fear or the assessment of intensity of basic emotions by children who experience war in Ukraine. Still, it is important to continue studying the long-term consequences of military conflicts in order to deepen the understanding of their impact on human mental functioning.
Blockchain Technology: Revolutionizing Transactions in the Digital Age
Authors: Bennet Daniel , Maria Lily , Putri Ayu Sanjaya Yulia , Rahmania Az Zahra Achani 
Published: May 2024

Blockchain technology emerges as a transformative innovation reshaping traditional transactional processes, eliminating intermediaries, enhancing security, and improving trust through its decentralized and transparent ledger system. However, challenges such as scalability and regulatory concerns hinder widespread adoption. Concrete examples and statistics from research enhance clarity regarding Blockchain's impacts, elaborating on how these challenges directly affect the adoption of Blockchain Technology in digital transactions. By analyzing case studies and current trends, this research underscores the efficiency and reliability of Blockchain implementation compared to traditional methods, advocating for widespread adoption to foster transparency, efficiency, and trust across diverse sectors. It emphasizes the necessity for organizations to adapt to stay competitive in the increasingly complex digital landscape, suggesting that a better understanding of Blockchain's impacts and challenges will aid organizations in taking strategic steps towards its long-term adoption.
Optical and electrical evaluation methods of plasma-induced damage in InP substrates
Authors: Goya Takahiro , Urabe Keiichiro , Eriguchi Koji 
Published: May 2024

Abstract
Indium phosphide (InP) has been focused on as one of the emerging materials that can be implemented in advanced semiconductor devices. We proposed optical and electrical characterization methods to evaluate plasma-induced physical damage (PPD)—ion bombardment damage—to InP substrates. By introducing a native oxide phase in an interfacial layer, we proposed an optical model of the damaged structure applicable for in-line monitoring by spectroscopic ellipsometry. Gas species dependence was obtained, which suggested that the H2 plasma exposure formed a thicker damaged layer than Ar. Impedance spectroscopy (IS) under various biases (V
b) was implemented to reveal the nature of damaged structures. Capacitive and conductive components assigned by the IS were confirmed to depend on incident species from plasma, indicating the difference in the energy profile of created defects. The presented methods are useful to characterize and control PPD in designing future high-performance InP-based devices.