bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using stable isotopes to track frugivory in migratory passerines
Authors: Gagnon Chantal , Hobson Keith A. 
Published: November 2009

Several species of North American migratory songbirds undergo seasonal diet shifts from insects to fruits, but this phenomenon is poorly quantified. Measurement of naturally occurring stable isotopes of carbon (δ13C) and nitrogen (δ15N) are linked to sources of diets and trophic level, respectively. We used stable carbon (δ13C) and nitrogen (δ15N) isotope analyses of blood and claw tissues of 16 species of migratory songbirds to evaluate the timing and extent of frugivory over different periods. Species differed considerably in their tissue δ15N values, but we found poor isotopic segregation of species according to our a priori classifications as insectivores or omnivores. Season accounted for considerable variance in tissue δ15N values. However, only American Robin ( Turdus migratorius L., 1766), Northern Oriole ( Icterus galbula (L., 1758)), Gray Catbird ( Dumetella carolinensis (L., 1766)), Least Flycatcher ( Empidonax minimus (W.M. Baird and S.F. Baird, 1843)), and Warbling Vireo ( Vireo gilvus (Vieillot, 1808)) showed expected decrease in winter-grown tissue δ15N values compared with those grown in late summer. This indicates either that our a priori guild associations were incorrect and (or) that using stable isotopes to track frugivory at continental scales is problematic. We recommend that the isotope technique be used to track frugivory only in well-constrained systems where food-web δ15N follows reliable and understood trophic enrichment patterns.
Foods and nutritional components of diets of black bear in Rocky Mountain National Park, Colorado
Authors: Baldwin Roger A. , Bender Louis C. 
Published: November 2009

We used scat analysis to determine diets and relative nutritional values of diets for black bears ( Ursus americanus Pallas, 1780) in Rocky Mountain National Park, Colorado, from 2003 to 2006, and compared foods consumed and nutritional components to identify important sources of fecal gross energy (GE), crude fat (CF), and fecal nitrogen (FN) in annual and seasonal diets. Patterns of use of food classes followed typical seasonal patterns for bears, although use of animal matter was among the highest reported (>49% annually). Use of animal matter increased after spring, although crude protein levels in bear diets were always >25%. GE was typically lowest for grasses and other herbaceous plants and highest for ants and ungulates; FN was strongly positively related to most animal sources, but negatively correlated with vegetative matter; and CF showed the strongest positive relationship with ungulates and berries, with the latter likely influenced by the presence of seeds. Compared with historic data (1984–1991), contemporary diets included substantially greater prevalence of anthropogenic foods, which likely contributed to increases in size, condition, and productivity of the contemporary bear population. Management strategies are needed to increase quantity and quality of natural foods while minimizing dependence on anthropogenic sources.
Seasonal detection rates of river otters (Lontra canadensis) using bridge-site and random-site surveys
Authors: Crimmins S. M. , Roberts N. M. , Hamilton D. A. , Mynsberge A. R. 
Published: November 2009

Randomization of survey sites is generally desired because of its unbiased approach, but is often abandoned because of logistical constraints. This is true for river otters ( Lontra canadensis (Schreber, 1777)), with bridges commonly determining survey locations. We conducted seasonal sign surveys for river otters on two rivers in southern Missouri, USA, using randomized survey points and fixed bridge-crossing points in 2001–2003. Otter sign was more likely to be detected at randomized sites than at bridge sites in summer (P < 0.0001), with sign being detected on 68% of visits to random sites (n = 348) and on 40% of visits to bridge sites (n = 60). Scat abundance was higher (P = 0.0001) at random sites (8.82 ± 0.6, mean ± SE) than at bridge sites (3.96 ± 1.0) during the summer. Similar but nonsignificant trends were found during the winter. Detection probabilities were significantly higher at random sites than at bridge sites in both seasons. Our results indicate that surveys of bridge sites for river otters may yield inaccurate results for distribution and relative abundance, particularly if conducted during the summer.
Forming foci of transmission: the effects of resource utilization, species interaction, and parasitism on molluscan movement
Authors: Gray T. T. , Detwiler J. T. , Minchella D. J. 
Published: November 2009

Animal aggregation to environmental cues provides opportunities for parasite transmission between individual hosts of the same or different species. Better characterization of host behavioral responses to environmental stimuli in the absence and presence of parasites will improve our understanding of how foci of transmission form. The behavioral response patterns of two co-occurring freshwater snail species ( Lymnaea elodes (Say, 1821) and Helisoma trivolvis (Say, 1817) (= Planorbella trivolvis (Say, 1817))) were assessed in response to three environmental stimuli (crayfish (genus Orconectes Cope, 1872) carrion, vegetation, or temperature gradient). Experiments were conducted with single species and species interactions. In addition, parasitized L. elodes were included in a single-species experiment and a species-interaction experiment. Snail species differed in the direction and magnitude of their responses to the environmental stimuli. Species interactions did not affect the responses to two of the stimuli for either species; however, interspecific interactions affected the response to high temperature in both species. Behavioral responses were altered in the presence of parasites for both the infected and uninfected hosts, suggesting parasitism is an important biotic factor in animal movement. This experimental study indicates co-occurring species respond to environmental factors in different ways. Furthermore, species interactions and parasitism within a guild can have strong effects on animal movement and potentially on parasite transmission.
Tapeworm (Hymenolepis diminuta) infection in flour beetles (Tribolium confusum): does it cause a trade-off between host fecundity and egg size?
Authors: Shostak Allen W. 
Published: November 2009

Inter- and intra-specific comparisons commonly reveal an inverse relationship between fecundity and offspring size. Many animals also vary egg size in response to environmental conditions. Infection of flour beetles Tribolium confusum Jaquelin Du Val, 1868 with the rat tapeworm Hymenolepis diminuta (Rudolphi, 1819) causes a major reduction in host fecundity. This study tested if this fecundity reduction was associated with changes in host egg size. Age-matched beetles were either fasted and then exposed to parasites, fasted only, or neither, and egg production and egg length determined for 5 weeks postexposure. Control beetles that were neither fasted nor exposed to parasites had steady egg production, but produced smaller eggs as they aged. Beetles that were fasted only, produced fewer but larger eggs for 1–2 weeks after the fast ended. Then, fecundity and egg size returned to control levels. Infected beetles also produced fewer, larger eggs for 1–2 weeks, but at levels indistinguishable from beetles that had been fasted only. After 2 weeks, while fecundity of infected beetles remained low, egg size became similar to noninfected hosts. Beetles appeared to trade-off fecundity and egg size in response to reduced feeding, but not to the presumed nutritional stress of parasitic infection.
Irrelevant Singletons in Visual Search Do Not Capture Attention but Can Produce Nonspatial Filtering Costs
Authors: Wykowska Agnieszka , Schubö Anna 
Published: November 2009

Abstract
It is not clear how salient distractors affect visual processing. The debate concerning the issue of whether irrelevant salient items capture spatial attention [e.g., Theeuwes, J., Atchley, P., & Kramer, A. F. On the time course of top–down and bottom–up control of visual attention. In S. Monsell & J. Driver (Eds.), Attention and performance XVIII: Control of cognitive performance (pp. 105–124). Cambridge, MA: MIT Press, 2000] or produce only nonspatial interference in the form of, for example, filtering costs [Folk, Ch. L., & Remington, R. Top–down modulation of preattentive processing: Testing the recovery account of contingent capture. Visual Cognition, 14, 445–465, 2006] has not yet been settled. The present ERP study examined deployment of attention in visual search displays that contained an additional irrelevant singleton. Display-locked N2pc showed that attention was allocated to the target and not to the irrelevant singleton. However, the onset of the N2pc to the target was delayed when the irrelevant singleton was presented in the opposite hemifield relative to the same hemifield. Thus, although attention was successfully focused on the target, the irrelevant singleton produced some interference resulting in a delayed allocation of attention to the target. A subsequent probe discrimination task allowed for locking ERPs to probe onsets and investigating the dynamics of sensory gain control for probes appearing at relevant (target) or irrelevant (singleton distractor) positions. Probe-locked P1 showed sensory gain for probes positioned at the target location but no such effect for irrelevant singletons in the additional singleton condition. Taken together, the present data support the claim that irrelevant singletons do not capture attention. If they produce any interference, it is rather due to nonspatial filtering costs.
Neural Changes Associated with Nonspeech Auditory Category Learning Parallel Those of Speech Category Acquisition
Authors: Liu Ran , Holt Lori L. 
Published: November 2009

Abstract
Native language experience plays a critical role in shaping speech categorization, but the exact mechanisms by which it does so are not well understood. Investigating category learning of nonspeech sounds with which listeners have no prior experience allows their experience to be systematically controlled in a way that is impossible to achieve by studying natural speech acquisition, and it provides a means of probing the boundaries and constraints that general auditory perception and cognition bring to the task of speech category learning. In this study, we used a multimodal, video-game-based implicit learning paradigm to train participants to categorize acoustically complex, nonlinguistic sounds. Mismatch negativity (MMN) responses to the nonspeech stimuli were collected before and after training, and changes in MMN resulting from the nonspeech category learning closely resemble patterns of change typically observed during speech category learning. This suggests that the often-observed “specialized” neural responses to speech sounds may result, at least in part, from the expertise we develop with speech categories through experience rather than from properties unique to speech (e.g., linguistic or vocal tract gestural information). Furthermore, particular characteristics of the training paradigm may inform our understanding of mechanisms that support natural speech acquisition.
Hyporesponsive Reward Anticipation in the Basal Ganglia following Severe Institutional Deprivation Early in Life
Authors: Mehta Mitul A. , Gore-Langton Emma , Golembo Nicole , Colvert Emma , Williams Steven C. R. , Sonuga-Barke Edmund 
Published: November 2009

AbstractSevere deprivation in the first few years of life is associated with multiple difficulties in cognition and behavior. However, the brain basis for these difficulties is poorly understood. Structural and functional neuroimaging studies have implicated limbic system structures as dysfunctional, and one functional imaging study in a heterogeneous group of maltreated individuals has confirmed the presence of abnormalities in the basal ganglia. Based on these studies and known dopaminergic abnormalities from studies in experimental animals using social isolation, we used a task of monetary reward anticipation to examine the functional integrity of brain regions previously shown to be implicated in reward processing. Our sample included a group of adolescents (n = 12) who had experienced global deprivation early in their lives in Romania prior to adoption into UK families. In contrast to a nonadopted comparison group (n = 11), the adoptees did not recruit the striatum during reward anticipation despite comparable performance accuracy and latency. These results show, for the first time, an association between early institutional deprivation and brain reward systems in humans and highlight potential neural vulnerabilities resulting from such exposures.
Sound Categorization and Conceptual Priming for Nonlinguistic and Linguistic Sounds
Authors: Aramaki Mitsuko , Marie Céline , Kronland-Martinet Richard , Ystad Sølvi , Besson Mireille 
Published: November 2009

Abstract
The aim of these experiments was to compare conceptual priming for linguistic and for a homogeneous class of nonlinguistic sounds, impact sounds, by using both behavioral (percentage errors and RTs) and electrophysiological measures (ERPs). Experiment 1 aimed at studying the neural basis of impact sound categorization by creating typical and ambiguous sounds from different material categories (wood, metal, and glass). Ambiguous sounds were associated with slower RTs and larger N280, smaller P350/P550 components, and larger negative slow wave than typical impact sounds. Thus, ambiguous sounds were more difficult to categorize than typical sounds. A category membership task was used in Experiment 2. Typical sounds were followed by sounds from the same or from a different category or by ambiguous sounds. Words were followed by words, pseudowords, or nonwords. Error rate was highest for ambiguous sounds and for pseudowords and both elicited larger N400-like components than same typical sounds and words. Moreover, both different typical sounds and nonwords elicited P300 components. These results are discussed in terms of similar conceptual priming effects for nonlinguistic and linguistic stimuli.
Characterisation of microRNA expression in post-natal mouse mammary gland development
Authors: Avril-Sassen Stefanie , Goldstein Leonard D , Stingl John , Blenkiron Cherie , Le Quesne John , Spiteri Inmaculada , Karagavriilidou Konstantina , Watson Christine J , Tavaré Simon , Miska Eric A , Caldas Carlos 
Published: November 2009

Abstract

Background
The differential expression pattern of microRNAs (miRNAs) during mammary gland development might provide insights into their role in regulating the homeostasis of the mammary epithelium. Our aim was to analyse these regulatory functions by deriving a comprehensive tissue-specific combined miRNA and mRNA expression profile of post-natal mouse mammary gland development.
We measured the expression of 318 individual murine miRNAs by bead-based flow-cytometric profiling of whole mouse mammary glands throughout a 16-point developmental time course, including juvenile, puberty, mature virgin, gestation, lactation, and involution stages. In parallel whole-genome mRNA expression data were obtained.

Results
One third (n = 102) of all murine miRNAs analysed were detected during mammary gland development. MicroRNAs were represented in seven temporally co-expressed clusters, which were enriched for both miRNAs belonging to the same family and breast cancer-associated miRNAs. Global miRNA and mRNA expression was significantly reduced during lactation and the early stages of involution after weaning. For most detected miRNA families we did not observe systematic changes in the expression of predicted targets. For miRNA families whose targets did show changes, we observed inverse patterns of miRNA and target expression. The data sets are made publicly available and the combined expression profiles represent an important community resource for mammary gland biology research.

Conclusion
MicroRNAs were expressed in likely co-regulated clusters during mammary gland development. Breast cancer-associated miRNAs were significantly enriched in these clusters. The mechanism and functional consequences of this miRNA co-regulation provide new avenues for research into mammary gland biology and generate candidates for functional validation.
Partial loss of heterozygosity events at the mutated gene in tumors from MLH1/MSH2 large genomic rearrangement carriers
Authors: Zavodna Katarina , Krivulcik Tomas , Bujalkova Maria Gerykova , Slamka Tomas , Martinicky David , Ilencikova Denisa , Bartosova Zdena 
Published: November 2009

Abstract

Background
Depending on the population studied, large genomic rearrangements (LGRs) of the mismatch repair (MMR) genes constitute various proportions of the germline mutations that predispose to hereditary non-polyposis colorectal cancer (HNPCC). It has been reported that loss of heterozygosity (LOH) at the LGR region occurs through a gene conversion mechanism in tumors from MLH1/MSH2 deletion carriers; however, the converted tracts were delineated only by extragenic microsatellite markers. We sought to determine the frequency of LGRs in Slovak HNPCC patients and to study LOH in tumors from LGR carriers at the LGR region, as well as at other heterozygous markers within the gene to more precisely define conversion tracts.

Methods
The main MMR genes responsible for HNPCC, MLH1, MSH2, MSH6, and PMS2, were analyzed by MLPA (multiplex ligation-dependent probe amplification) in a total of 37 unrelated HNPCC-suspected patients whose MLH1/MSH2 genes gave negative results in previous sequencing experiments. An LOH study was performed on six tumors from LGR carriers by combining MLPA to assess LOH at LGR regions and sequencing to examine LOH at 28 SNP markers from the MLH1 and MSH2 genes.

Results
We found six rearrangements in the MSH2 gene (five deletions and dup5-6), and one aberration in the MLH1 gene (del5-6). The MSH2 deletions were of three types (del1, del1-3, del1-7). We detected LOH at the LGR region in the single MLH1 case, which was determined in a previous study to be LOH-negative in the intragenic D3S1611 marker. Three tumors displayed LOH of at least one SNP marker, including two cases that were LOH-negative at the LGR region.

Conclusion
LGRs accounted for 25% of germline MMR mutations identified in 28 Slovakian HNPCC families. A high frequency of LGRs among the MSH2 mutations provides a rationale for a MLPA screening of the Slovakian HNPCC families prior scanning by DNA sequencing. LOH at part of the informative loci confined to the MLH1 or MSH2 gene (heterozygous LGR region, SNP, or microsatellite) is a novel finding and can be regarded as a partial LOH. The conversion begins within the gene, and the details of conversion tracts are discussed for each case.
The Int7G24A variant of transforming growth factor-beta receptor type I is a risk factor for colorectal cancer in the male Spanish population: a case-control study
Authors: Castillejo Adela , Mata-Balaguer Trinidad , Guarinos Carla , Castillejo María-Isabel , Martínez-Cantó Ana , Barberá Víctor-Manuel , Montenegro Paola , Ochoa Enrique , Lázaro Rafael , Guillén-Ponce Carmen , Carrato Alfredo , Soto José-Luís 
Published: November 2009

Abstract

Background
The Int7G24A variant of transforming growth factor-beta receptor type I (TGFBR1) has been shown to increase the risk for kidney, ovarian, bladder, lung and breast cancers. Its role in colorectal cancer (CRC) has not been established. The aims of this study were to assess the association of TGFBR1*Int7G24A variant with CRC occurrence, patient age, gender, tumour location and stage.

Methods
We performed a case-control study with 504 cases of sporadic CRC; and 504 non-cancerous age, gender and ethnically matched controls. Genotyping analysis was performed using allelic discrimination assay by real time PCR.

Results
The Int7G24A variant was associated with increased CRC incidence in an additive model of inheritance (P for trend = 0.005). No significant differences were found between Int7G24A genotypes and tumour location or stage. Interestingly, the association of the Int7G24A variant with CRC risk was significant in men (odds ratio 4.10 with 95% confidence intervals 1.41-11.85 for homozygous individuals; P for trend = 0.00023), but not in women. We also observed an increase in susceptibility to CRC for individuals aged less than 70 years.

Conclusion
Our data suggest that the Int7G24A variant represents a risk factor for CRC in the male Spanish population.
Quantifying and resolving multiple vector transformants in S. cerevisiae plasmid libraries
Authors: Scanlon Thomas C , Gray Elizabeth C , Griswold Karl E 
Published: November 2009

Abstract

Background
In addition to providing the molecular machinery for transcription and translation, recombinant microbial expression hosts maintain the critical genotype-phenotype link that is essential for high throughput screening and recovery of proteins encoded by plasmid libraries. It is known that Escherichia coli cells can be simultaneously transformed with multiple unique plasmids and thusly complicate recombinant library screening experiments. As a result of their potential to yield misleading results, bacterial multiple vector transformants have been thoroughly characterized in previous model studies. In contrast to bacterial systems, there is little quantitative information available regarding multiple vector transformants in yeast. Saccharomyces cerevisiae is the most widely used eukaryotic platform for cell surface display, combinatorial protein engineering, and other recombinant library screens. In order to characterize the extent and nature of multiple vector transformants in this important host, plasmid-born gene libraries constructed by yeast homologous recombination were analyzed by DNA sequencing.

Results
It was found that up to 90% of clones in yeast homologous recombination libraries may be multiple vector transformants, that on average these clones bear four or more unique mutant genes, and that these multiple vector cells persist as a significant proportion of library populations for greater than 24 hours during liquid outgrowth. Both vector concentration and vector to insert ratio influenced the library proportion of multiple vector transformants, but their population frequency was independent of transformation efficiency. Interestingly, the average number of plasmids born by multiple vector transformants did not vary with their library population proportion.

Conclusion
These results highlight the potential for multiple vector transformants to dominate yeast libraries constructed by homologous recombination. The previously unrecognized prevalence and persistence of multiply transformed yeast cells have important implications for yeast library screens. The quantitative information described herein should increase awareness of this issue, and the rapid sequencing approach developed for these studies should be widely useful for identifying multiple vector transformants and avoiding complications associated with cells that have acquired more than one unique plasmid.