bluuebunny/crossref_metadata_2025_split huggingface dataset:
Behavioral comorbidities treatment by fecal microbiota transplantation in canine epilepsy: a pilot study of a novel therapeutic approach
Authors: Watanangura Antja , Meller Sebastian , Farhat Nareed , Suchodolski Jan S. , Pilla Rachel , Khattab Mohammad R. , Lopes Bruna C. , Bathen-Nöthen Andrea , Fischer Andrea , Busch-Hahn Kathrin , Flieshardt Cornelia , Gramer Martina , Richter Franziska , Zamansky Anna , Volk Holger A. 
Published: June 2024

IntroductionAnxiety and cognitive dysfunction are frequent, difficult to treat and burdensome comorbidities in human and canine epilepsy. Fecal microbiota transplantation (FMT) has been shown to modulate behavior in rodent models by altering the gastrointestinal microbiota (GIM). This study aims to investigate the beneficial effects of FMT on behavioral comorbidities in a canine translational model of epilepsy.MethodsNine dogs with drug-resistant epilepsy (DRE) and behavioral comorbidities were recruited. The fecal donor had epilepsy with unremarkable behavior, which exhibited a complete response to phenobarbital, resulting in it being seizure-free long term. FMTs were performed three times, two weeks apart, and the dogs had follow-up visits at three and six months after FMTs. Comprehensive behavioral analysis, including formerly validated questionnaires and behavioral tests for attention deficit hyperactivity disorder (ADHD)- and fear- and anxiety-like behavior, as well as cognitive dysfunction, were conducted, followed by objective computational analysis. Blood samples were taken for the analysis of antiseizure drug (ASD) concentrations, hematology, and biochemistry. Urine neurotransmitter concentrations were measured. Fecal samples were subjected to analysis using shallow DNA shotgun sequencing, real-time polymerase chain reaction (qPCR)-based Dysbiosis Index (DI) assessment, and short-chain fatty acid (SCFA) quantification.ResultsFollowing FMT, the patients showed improvement in ADHD-like behavior, fear- and anxiety-like behavior, and quality of life. The excitatory neurotransmitters aspartate and glutamate were decreased, while the inhibitory neurotransmitter gamma-aminobutyric acid (GABA) and GABA/glutamate ratio were increased compared to baseline. Only minor taxonomic changes were observed, with a decrease in Firmicutes and a Blautia_A species, while a Ruminococcus species increased. Functional gene analysis, SCFA concentration, blood parameters, and ASD concentrations remained unchanged.DiscussionBehavioral comorbidities in canine IE could be alleviated by FMT. This study highlights FMT’s potential as a novel approach to improving behavioral comorbidities and enhancing the quality of life in canine patients with epilepsy.
HIV latency potential may be influenced by intra-subtype genetic differences in the viral long-terminal repeat
Authors: Doolabh Deelan Sudhir , Selhorst Philippe , Williamson Carolyn , Chopera Denis , Abrahams Melissa-Rose 
Published: June 2024

IntroductionElucidation of mechanisms that drive HIV latency is essential to identifying cure strategies. While host mechanisms associated with viral persistence on antiretroviral therapy (ART) have been well studied, less is known about the viral properties that influence latency. The viral promoter element, the 5’ long terminal repeat (LTR), has been shown to affect the number of latently infected cells shortly after infection. Here we investigated the role of subtype C LTR genotypic variation on the establishment of latency in a dual reporter HIV-1 infection model.MethodsThe LTR U3 and R regions from 11 women with acute/early subtype C HIV infection were cloned into the dual reporter pRGH plasmid. Latency potential was calculated based on the expression of fluorescent reporter genes in Jurkat E6–1 cells measured by flow cytometry as the proportion of latent (mCherry +ve cells)/proportion of active (eGFP +ve mCherry +ve cells) infection. Reversal of latency was performed using PMA/Ionomycin stimulation 24 hours before fixing of cells. LTR transcriptional capacity, in the presence and absence of a heterologous subtype C Tat, was measured for the same LTRs cloned into a pGL4.10 luciferase expression vector following transfection of HEK293T cells.ResultsThe majority of proviruses were latent at day 8 post-infection, yet the proportion of latently infected cells varied significantly across participants. We observed a median latent:active infection ratio of 1.79 (range 0.86–2.83) across LTRs with the hierarchy of latency potential remaining consistent across repeat experiments. The median latent:active infection ratio decreased by a median of 3-fold following PMA/Ionomycin stimulation to 0.55 (range 0.46–0.78) indicating that a proportion of latently infected cells could produce viral proteins upon activation. Latency potential did not correlate with LTR transcriptional capacity.ConclusionsWe found intra-subtype level differences in the latency potential of LTRs from South African women independent of their transcriptional capacity, suggesting that HIV-1 LTRs have intrinsic properties that influence the proportion of latently infected cells shortly after infection. The inability to reactivate viral expression in all latently infected cells supports the complex nature of mechanisms driving latency and the need for continued advancements in methods used to study these mechanisms.
Avoidable Mortality in Korea 1997–2001: Temporal Trend and its Contribution to All-cause Mortality
Authors: Jeong Yoolwon , Seo Sunghyo 
Published: June 2024

ObjectiveThis study analyzed the mortality trends from avoidable causes in Korea from 1997 to 2021, to estimate its contribution to the overall mortality in different subgroups, including. Gender, age, and cause of disease.MethodsThe all-cause and avoidable mortality were presented as a time series plot and average annual percent change. Trend of avoidable mortality was also analyzed by subgroups, disease causes and the percentage attributed to each causes.ResultsThe decline in avoidable mortality accounted for 82.6% of all-cause mortality reduction. Preventable mortality showed a more pronounced decline than treatable mortality, explaining 72.3% of the avoidable mortality reduction. In 1997–2001, avoidable death occurred in 72.2% (537,024 cases) of all-cause deaths, which declined to 60.0% (342,979 cases) in 2017–2021. The contribution of avoidable mortality in the decline of all-cause morality was greater in males (83.6%) than in females (79.3%).ConclusionThe decline in avoidable mortality and its contribution to the all-cause mortality reduction implies general improvement of the population health in Korea. Nevertheless, the heterogenous trend within different subgroups warrants more equitable design and implementation of health services and policies.
Views, Attitudes and Challenges When Supporting a Family Member in Their Decision to Travel to Switzerland to Receive Aid-In-Dying
Authors: Sperling Daniel 
Published: June 2024

ObjectivesExploring the experiences, perceptions and meanings of family members and close friends of Israeli individuals who sought aid-in-dying outside Israel.MethodsUsing the phenomenological-interpretive approach, a qualitative research design was employed, based on ten in-depth semi-structured interviews with Israelis who had provided support for a relative who embarked on suicide tourism.ResultsThe following five themes emerged from interviews: (1) facilitators for supporting an individual requesting suicide tourism; (2) choosing death and actively making the decision to die; (3) the meaning of traveling to die; (4) offering support throughout the process; and (5) facilitating procedures after death.ConclusionThe participants spoke of the active role that they played in their relative’s suicide-tourism journey. They conveyed conflicting emotions and values regarding the decision at hand, the ability to say goodbye thanks to their pre-planned death, helping to reduce their suffering and burden, and dealing with the challenge of disclosing the deceased’s plans, before and after the act, as well as their own involvement in the process. Relatives of suicide-tourism patients should receive professional support during and following this difficult process.
Passion Fruit Cultivation: An Approach to Internal Leaf Anatomy
Authors: Manzani Lisboa Lucas Aparecido , Cavichioli José Carlos , Ferreira Thiago de Souza , da Silva Aldeir , de Figueiredo Paulo Alexandre Monteiro 
Published: June 2024

Some species of the genus Passiflora have leaf morphological adaptations that grow to influence the development of the plant in producing areas. Hence, the objective of this work is to quantify and characterize the leaf anatomy of passion fruit species distributed in the South American region, which can become an important strategy in the selection of species more adapted to the environment where they will be grown. This work evaluates the abaxial and adaxial cuticle thickness (ABCT and ADCT), abaxial and adaxial epidermis thickness (ABET and ADET), xylem diameter (XD), phloem diameter (PD), and thickness of the palisade parenchyma (TPP), of the species Passiflora quadrangularis L., Passiflora foetida L., Passiflora edulis Sims, Passiflora gibertii N.E Brown, Passiflora coccinea Aubl, Passiflora alata Curtis, Passiflora tenuifila Killip, Passiflora caerulea L., and Passiflora cincinnata Mast. Passion fruit species present differences in leaf anatomy, which may influence the plant’s development. The species Passiflora quadrangularis L. showed a greater thickness of cuticles, epidermis, conducting vessels, and palisade parenchyma. The species Passiflora edulis has higher density and stomatal functionality. All Passiflora species formed druses on their leaves.
Functional assessment of microcirculation in the injection site tissues when using local anaesthesia
Authors: Anisimova E. N. , Anisimova N. U. , Orekhova I. V. , Pershina L. V. 
Published: June 2024

Relevance. In modern dentistry, the primary criteria for successful anaesthesia are the effectiveness and safety of the anaesthetic used. These factors depend on the type of local anaesthetic and the method of its administration in the maxillofacial tissues. Studying the impact of locally anaesthetizing solutions administered by different injection techniques on the periodontal tissues is possible by recording changes in microcirculation using the LDF method.Purpose. To dynamically record the baseline microcirculation indicator in the tissues at the injection site when using various methods and means of local anaesthesia.Materials and methods. The study of changes in the microcirculation of periodontal tissues was conducted among 105 patients: 67 women and 38 men aged 25 to 56 years without periodontal pathology and comorbidities, using injection methods of anaesthesia (infiltration and modified periodontal anaesthesia) with 2% and 4% solutions of articaine with varying concentrations of vasoconstrictor. Changes in the microcirculation index were recorded at the injection site: at the root apex projection during infiltration and in the gingival papilla region during MPA, with an exposure time during LDF registration of 5 minutes. To maintain the fiber optic probe, an individual key made of C-silicone with an additional corrective layer was used for repeat positioning. The most informative indicator of the study was the magnitude of the average blood perfusion flow (M) in the time interval of registration, with its changes reflected in the study results.Results. Analysis of the dynamics of microcirculation indicators in the injection site tissues when using various methods of administering locally anaesthetizing drugs based on 2% and 4% solutions of articaine without vasoconstrictor and with epinephrine at concentrations of 1:200,000 and 1:100,000 allowed the determination of tissue ischemia depth at the injection site and the rate of blood flow restoration after anaesthesia.Conclusion. Changes in microcirculation indicators in the tissues at the injection site of the local anaesthetic depend on the concentration of the local anaesthetic and vasoconstrictor, the method of administration used, and the volume of the drug administered.
Investigation of the ultrastructure of dental pulp odontoblasts in a patient following a COVID-19 viral infection
Authors: Pugacheva A. A. , Orekhova L. Yu. , Oksas N. S. , Paramonova N. M. , Kudryavtseva T. V. 
Published: June 2024

Relevance. Recent studies on odontoblast ultrastructure have indicated that these cells possess immune functions. Unfortunately, both domestic and international sources lack transmission electron microscopy research data that characterise the ultrastructure of odontoblasts. This gap in the literature limits the expansion of knowledge about these specific cells of the human dental pulp and their practical applications. An analysis of existing literature and our own research suggests that the organs of the oral cavity may become targets for the coronavirus. Odontoblasts, as specialized cells of the dental pulp, perform multiple functions. Their primary role is in dentinogenesis, but equally important is their participation in immune functions, evidenced by the presence of pattern recognition receptors (PRRs) on their surface. These include Toll-like receptors (TLRs) and nucleotide-binding oligomerization domain-like receptors (NOD-like receptors, NLRs), some of which can mount an immune response to the entry of SARS-CoV-2 into the cell.Materials and methods. This study involved a comprehensive clinical-laboratory investigation of the pulp from an intact tooth 2.8, extracted for orthodontic reasons from a patient who had recovered from a COVID-19 infection a few weeks prior to the tooth extirpation. The ultrastructure of the dental pulp was examined using transmission electron microscopy.Results. In the ultrathin sections of the pulp specimens from the extirpated tooth, specific odontoblast cells with clearly traceable ultrastructure were identified. The electron micrographs visualized features characteristic of viral entry into the cell: initial apoptotic changes in the nucleus of the odontoblast, the presence of clathrin-coated vesicles in the cytoplasmic membrane of the odontoblast process. Additionally, clathrin-coated invaginations of the cytolemma in the terminal parts of the odontoblasts were observed.Conclusion. The electron micrographs revealed ultrastructural changes indicative of a viral infection, suggesting the involvement of odontoblasts, equipped with the TLR3 and TLR4 system, in the antiviral defence against SARS-CoV-2.
Síndrome de Conn: presentación de un caso
Authors: Umaña Venegas Alice , García Quirós Christian 
Published: June 2024

Se estudia el caso de una paciente femenina de 33 años, conocida hipertensa desde los 17 años, con reciente tratamiento antihipertensivo, con historia de dos gestas sin antecedente de preeclamsia, quien es remitida de la consulta de medicina familiar y comunitaria al tercer nivel de atención por hipertensión resistente a tratamiento y quien refiriendo cefalea ocasional, edema periférico matutino y palpitaciones autolimitadas, así como una hipokalemia leve e intermitente, por lo cual la paciente se encontraba en tratamiento con 4 medicamentos antihipertensivos. Debido al alto indice de sospecha clínica de hiperaldosteronismo primario se realizó un cribado con la relación aldosterona / renina, el cual se reportó de 29.2 en reposo y 36.7 de pie, asociado a una tomografía axial computarizada de abdomen en el que se reportó una imagen nodular en la glándula suprarrenal izquierda, siendo altamente sugestiva de un adenoma productor de aldosterona. Se decide ingresar y realizar adrenalectomia izquierda, cuya biopsia reportó una masa en la glándula suprarrenal de color amarillo y aspecto heterogéneo, concordante con un adenoma cortical izquierdo productor de aldosterona.
Uso del implante anticonceptivo subdérmico en adolescentes: revisión bibliográfica.
Authors: Ureña Fernández Ashley Melania 
Published: June 2024

Mundialmente se ha establecido el embarazo no deseado en la adolescencia como un problema de salud pública, situación que no es ajena a la población costarricense. Diferentes métodos anticonceptivos han sido implementados con el objetivo de disminuir esta incidencia. El objetivo principal del presente artículo radica en la realización de un análisis sistemático de información basado en evidencia científica, para determinar elementos de importancia relacionados con el uso del implante subdérmico como método anticonceptivo dirigido a la población adolescente sexualmente activa. La literatura utilizada consiste en artículos científicos extraídos de bases de datos confiables, libros de texto, circulares institucionales y registros sanitarios de Costa Rica.
El análisis efectuado permite situar el implante anticonceptivo subdérmico como método de elección de primera línea para la planificación familiar en adolescentes, tal y como lo respaldan el Colegio Americano de Ginecología y Obstetricia y la Academia Americana de Pediatría. La responsabilidad ineludible que le corresponde al médico de conocer la información existente sobre este método anticonceptivo y trasladarla de manera adecuada a la población adolescente sexualmente activa, puede determinar la elección de los implantes subdérmicos sobre otras alternativas terapéuticas y la adherencia durante todo el periodo recomendado. La posibilidad de aumentar el periodo de uso a más de tres años podría no solamente disminuir las molestias asociadas con la colocación y retiro del dispositivo, sino que además implicaría una posible disminución en términos de inversión económica para las instituciones de salud pública, lo que fundamenta la realización de estudios clínicos para determinar la viabilidad.
Actualización del tema: abordaje de la migraña en atención primaria
Authors: Quirós Pérez Jackeline , Herdoiza Morales Cristian Andres , Segnini Miranda Jorge Andres 
Published: June 2024

La migraña es una patología de carácter neurológico frecuente, donde interaccionan diferentes estructuras anatómicas con múltiples mecanismos fisiopatológicos que involucran la activación de la vía trigémino-vascular y que conllevan la aparición de 5 fases. Conocerlas resulta de gran importancia, ya que con ello se pueden explorar opciones terapéuticas. Existen ciertos criterios diagnósticos en el momento de evaluar al paciente con migraña, los cuales permiten un adecuado diagnóstico y posteriormente abordaje.
Las estrategias para el manejo óptimo de la migraña abarcan medidas no farmacológicas y farmacológicas. El tratamiento farmacológico agudo debe iniciarse de forma temprana, siguiendo una pauta personalizada y escalada en el manejo del dolor. El tratamiento preventivo representa un reto para los facultativos, dado que se suelen utilizar fármacos que originalmente se crearon con otro enfoque terapéutico, entre ellos se pueden encontrar antihipertensivos como los beta bloqueadores, antidepresivos tricíclicos y antiepilépticos. Recientemente, han florecido nuevas terapias innovadoras en el manejo de la migraña, como los anticuerpos monoclonales dirigidos hacia CGRP. Medicamentos como erenumab, fremanezmab, galcanezumab y eptinezumab han surgido como opciones en la prevención de episodios migrañosos específicamente en esta vía bioquímica.
Sistem Pendukung Keputusan Pemilihan Cafe di Kota Padang Menggunakan Metode Weighted Product
Authors: Aulia Aulia Putri , Putri Yani  , Roki Juliska  , Ami Anggraini Samudra  
Published: June 2024

Kota Padang merupakan salah satu kota tujuan wisata yang sangat popular di Sumatera Barat. Di Kota Padang terdapat begitu banyak tempat wisata, mulai dari pantai, gunung, pasar tradional, pasar modern, museum dan tempat lainnya yang menarik perhatian wisatawan untuk berkunjung. Dalam satu tahun terakhir, peningkatan wisatawan di Kota Padang mengalami kenaikan hingga 100%, sehingga ini meningkatkan peluang usaha yang baik bagi masyarakat Kota Padang khususnya yang berada pada titik lokasi wisata. Masyarakat yang melihat peluang ini berlomba-lomba mendirikan cafe. Banyaknya variasi cafe ini akan berbanding lurus dengan berbagai macam cafe dengan keberagaman penilaiannya, dengan ini pengunjung akan dibuat bingung dengan pilihan café yang ada di Kota Padang. Maka dari itu dibutuhkan sebuah Sistem Pendukung Keputusan yang dapat dapat membantu pengambilan keputusan melalui penggunaan data dan model-model keputusan untuk dapat memecahkan masalah pengunjung. Didalam penelitian digunakan 10 sampel cafe yang diuji dan menghasilkan cafe terbaik. Hasil dari penelitian ini terbukti bahwa metode weighted product dapat digunakan dalam proses pemilihan cafe terbaik di Kota Padang.
Metode Analytical Hierarchy Process
Authors: Ega Mahrifah  , Ami Anggraini Samudra  , Reni Handani  , Beni Mulya  , Randa Dwi Saputra5  
Published: June 2024

Telah dikembangkan sistem baru yang diimplementasikan dalam sebuah sistem penunjang keputusan. Sistem ini digunakan untuk membantu mempermudah pengolahan data dalam pemilihan tas. Seluruh pendataan yang berhubungan dalam pemilihan tas pada harga, model, kualitas. Metode yang digunakan untuk proses pemilihan tas tersebut menggunakan AHP (Analytic Hierarchi Process). Tahapan dalam metode AHP diawali proses pendefinisian masalah, pembuatan struktur hierarki yang diawali dengan tujuan umum, dilanjutkan dengan kriteria-kriteria dan alternatif- alternatif pilihan, Membuat matrik perbandingan berpasangan, Menghitung eigen vector dari setiap matriks perbandingan berpasangan, Menguji konsistensi hirarki.
Pengembangan Sistem Informasi Badan Usaha Milik Desa(Bumdes) Maju Rahayu Desa Jatinrunggo Menggunakan Odoo Berbasis Web Mobile
Authors: Hevi Alvina  , Sadam Rinto  , Silvi Meilani  , Hendri Saputra  , Kustiyono  
Published: June 2024

Abstrak
Village-Owned Enterprises (BUMDes) in Jatirunggo Village, Pringapus District, Semarang Regency are still manual. Some people do not know that there are Bumdes activities in Jatirunggo Village so planning needs to be done. An application that uses Internet services can be used by the public to obtain information and save time, so there is a need for a website application in Jatirunggo Village. The author designs and designs a mobile web-based Village Owned Enterprise information system using a website builder in the form of Odoo because it is considered very effective in designing information systems. Apart from that, the author also uses a computer along with supporting village information data obtained from BUMDes administrators. With the village's mobile web-based company information system, it is hoped that this will make it easier to obtain information and improve internal business marketing services in Jatirunggo Village which are managed by BUMDes.

Keywords: Information system; BUMDes; WebMobile

Abstrak
Badan Usaha Milik Desa (BUMDes) di Desa Jatirunggo Kecamatan Pringapus Kabupaten Semarang masih bersifat manual. Sebagian masyarakat belum mengetahui bahwa terdapat kegiatan Bumdes di Desa Jatirunggo sehingga perlu dilakukan perencanaan. Sebuah aplikasi yang menggunakan layanan Internet dapat digunakan masyarakat untuk memperoleh informasi dan dapat menghemat waktu, sehingga diperlukan adanya aplikasi website di Desa Jatirunggo. Penulis merancang dan merancang sistem informasi Badan Usaha Milik Desa berbasis mobile web dengan menggunakan website bulder berupa Odoo karena dianggap sangat efektif dalam perancangan sistem informasi, Selain itu penulis juga menggunakan computer berserta data-data pendukung informasi desa yang didapatkan dari pengurus BUMDes. Dengan adanya sistem informasi perusahaan berbasis mobile web milik desa Diharapkan hal ini dapat mempermudah mendapatkan informasi dan meningkatkan pelayanan internal Pemasaran usaha yang ada di Desa Jatirunggo yang dikelola oleh BUMDes.

Kata Kunci: Sistem informasi; BUMDes; Web Mobile