bluuebunny/crossref_metadata_2025_split huggingface dataset:
Brief communication: Y‐chromosome haplogroup analysis indicates that Chinese Tuvans share distinctive affinity with Siberian Tuvans
Authors: Chen Zheng , Zhang Yongke , Fan An , Zhang Yanan , Wu Yanping , Zhao Qianjun , Zhou Yong , Zhou Canlin , Bawudong M. , Mao Xinmin , Ma Yuehui , Yang Lingyun , Ding Yuanling , Wang Xueqin , Rao Shaoqi 
Published: January 2011

AbstractTuvans are mainly distributed in Siberia (the Republic of Tuva), Mongolia, and China. The genetic origin of Chinese Tuvans remains controversial. The Tuvans in China were classified as Mongolians in the early 1950s by the National Ethnic Affairs Commission of China, but they defined themselves as a separate group. To resolve this dispute and determine their genetic relationships with the peoples in Central Asia, we randomly selected 150 male subjects from the Tuvans in the Altai region of Xinjiang Uygur Autonomous Region in China. Fourteen Y chromosomal markers were genotyped using the RFLP method or direct sequencing. These haplogroup data were combined with public data for 15 populations in South Siberia and Central Asia. Tuvans in both China and the Republic of Tuva had the highest frequencies of haplogroups K‐M9 and Q‐M242. Principal component analysis demonstrated that the Tuvans in China were of a distinct cluster, separated from their neighbors, the Mongolians and Kazakhs, which finding was consistent with the Analysis of Molecular Variances. Further population tree analysis revealed that Tuvans were on a far‐separated cluster from their neighbors. Based on these results, we propose that the Tuvans (in both China and the Republic of Tuva) constitute a group distinct from Mongolians and from other Central Asia populations. However, the genetic results might be the consequence of some evolutionary forces like genetic drift and founder effect, and do not necessarily reflect their ultimate origin. Am J Phys Anthropol, 2011. © 2011 Wiley‐Liss, Inc.
Genetic structure of the paternal lineage of the Roma People
Authors: Pamjav Horolma , Zalán Andrea , Béres Judit , Nagy Melinda , Chang Yuet Meng 
Published: January 2011

AbstractAccording to written sources, Roma (Romanies, Gypsies) arrived in the Balkans around 1,000 years ago from India and have subsequently spread through several parts of Europe. Genetic data, particularly from the Y chromosome, have supported this model, and can potentially refine it. We now provide an analysis of Y‐chromosomal markers from five Roma and two non‐Roma populations (N = 787) in order to investigate the genetic relatedness of the Roma population groups to one another, and to gain further understanding of their likely Indian origins, the genetic contribution of non‐Roma males to the Roma populations, and the early history of their splits and migrations in Europe. The two main sources of the Roma paternal gene pool were identified as South Asian and European. The reduced diversity and expansion of H1a‐M82 lineages in all Roma groups imply shared descent from a single paternal ancestor in the Indian subcontinent. The Roma paternal gene pool also contains a specific subset of E1b1b1a‐M78 and J2a2‐M67 lineages, implying admixture during early settlement in the Balkans and the subsequent influx into the Carpathian Basin. Additional admixture, evident in the low and moderate frequencies of typical European haplogroups I1‐M253, I2a‐P37.2, I2b‐M223, R1b1‐P25, and R1a1‐M198, has occurred in a more population‐specific manner. Am J Phys Anthropol, 2011. © 2011 Wiley‐Liss, Inc.
Periodontal disease in a Portuguese identified skeletal sample from the late nineteenth and early Twentieth Centuries
Authors: Wasterlain Sofia N. , Cunha Eugénia , Hillson Simon 
Published: January 2011

AbstractPeriodontal status was investigated in 600 adult dentitions belonging to the identified osteological collections curated at the University of Coimbra, Portugal. These collections date to a point temporally intermediate between the large epidemiological studies of the 20th century and archaeological collections that antedate the 19th century. The aim of this study is to compare periodontal data derived from contemporary samples with statistics compiled from epidemiological studies to determine if factors such as age‐at‐death, sex, and tooth type are essential or not to account for in future archaeological studies of periodontitis. Periodontal disease status was assessed based on the textural and architectural variations of the interdental septum and the extent of bone loss. Overall, the frequency of periodontitis within the Coimbra collections is 73.8%. Men were more susceptible to periodontal disease than women. Gingivitis was widespread in the younger age groups. Destructive periodontitis was observed early in adulthood, rising steadily with age. The most susceptible sites to periodontal breakdown were located in the posterior region of the upper jaw. Some variation in reported frequencies of periodontitis was found in epidemiological reports, which might result from variation in methods for identifying the pathology, differences in the age composition of the samples examined, variation in oral hygiene and/or diet, or some other factors. Regarding the pattern of distribution of periodontal disease, Coimbra results were similar to comparable modern epidemiological surveys, making clear the importance of considering sex, age, and oral distribution of periodontitis in future archaeological studies. Am J Phys Anthropol, 2011. © 2011 Wiley‐Liss, Inc.
Challenging views on the peopling history of East Asia: The story according to HLA markers
Authors: Di Da , Sanchez‐Mazas Alicia 
Published: January 2011

AbstractThe peopling of East Asia by the first modern humans is strongly debated from a genetic point of view. A north‐south genetic differentiation observed in this geographic area suggests different hypotheses on the origin of Northern East Asian (NEA) and Southern East Asian (SEA) populations. In this study, the highly polymorphic HLA markers were used to investigate East Asian genetic diversity. Our database covers a total of about 127,000 individuals belonging to 84 distinct Asian populations tested for HLA‐A, ‐B, ‐C, ‐DPB1, and/or ‐DRB1 alleles. Many Chinese populations are represented, which have been sampled in the last 30 years but rarely taken into account in international research due to their data published in Chinese. By using different statistical methods, we found a significant correlation between genetics and geography and relevant genetic clines in East Asia. Additionally, HLA alleles appear to be unevenly distributed: some alleles observed in NEA populations are widespread at the global level, while some alleles observed in SEA populations are virtually unique in Asia. The HLA genetic variation in East Asia is also characterized by a decrease of diversity from north to south, although a reverse pattern appears when one only focuses on alleles restricted to Asia. These results reflect a more complex migration history than that illustrated by the “southern‐origin” hypothesis, as genetic contribution of ancient human migrations through a northern route has probably been quite substantial. We thus suggest a new overlapping model where northward and southward opposite migrations occurring at different periods overlapped. Am J Phys Anthropol, 2011. © 2011 Wiley‐Liss, Inc.
Ring‐opening polymerization of trimethylenecarbonate by the bridged diphenoxo ytterbium (II) complex
Authors: Zhao B. , Hu X. L. , Lu C. R. 
Published: January 2011

AbstractA novel ytterbium (II) complex 2 supporting by a bridged bisphenolate ligand H2LOC4H7 (L = C4H7OCH2N(CH2‐2‐OC6H2‐3,5‐Bu)2) with a tetrahydrofuran donor on side‐arm was synthesized in high yield and characterized by elementary analysis, IR, 1H‐NMR, and 13C‐NMR. Ring‐opening polymerization of 1,3‐trimethylenecarbonate (TMC) was carried out using complex 2 and a known complex [Me2NCH2CH2N(CH2‐2‐O‐3,5‐C6H2(But)2)2]Yb 1 as the initiators, respectively. It was found that both complexes 1 and 2 can alone initiate the ring‐opening polymerization of TMC, and complex 1 showed higher activity than complex 2. The activity of both complexes 1 and 2 was found to be higher than that of monodentate phenoxo ytterbium (II) complex (2,6‐Bu‐C6H3O)2Yb(THF)3. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Quantitative Three‐Dimensional Analysis of Embryonic Chick Morphogenesis Via Microcomputed Tomography
Authors: Kim Jun Sup , Min Jouha , Recknagel Andrew K. , Riccio Mark , Butcher Jonathan T. 
Published: January 2011

AbstractMicro‐computed tomographic imaging of a stage HH36 chick embryo. Signal intensity was pseudocolored to identify skeletal, vascular, and soft tissue components. See Kim et al., on page 1, in this issue.
In Situ Raman and Pulse Reaction Study on the Partial Oxidation of Methane to Synthesis Gas over a Pt/Al2O3 Catalyst
Authors: Wang Mei‐Liu , Zheng Hao‐Zhuan , Li Jian‐Mei , Weng Wei‐Zheng , Xia Wen‐Sheng , Huang Chuan‐Jing , Wan Hui‐Lin 
Published: January 2011

AbstractCatalytic partial oxidation of methane (POM) to synthesis gas (syngas) over Pt/Al2O3 was investigated by in situ microprobe Raman and pulse reaction methods with attention focused on the mechanism of syngas formation in the oxidation zone (i.e., the catalyst zone in which O2 was still available in the reaction feed). It was found that the amount of platinum oxide in the catalyst under POM conditions was below the detection level of Raman spectroscopy. Raman bands of carbon species that originated from methane dissociation were detected at the entrance of the catalyst bed under working conditions. The results of the pulse reaction study on POM as well as steam and CO2 reforming of methane at 700 °C with a contact time of less than 1 ms over the catalyst suggest that pyrolysis of methane on reduced platinum sites followed by coupling of two surface hydrogen atoms to H2 and partial oxidation of surface carbon species to CO are the major reactions responsible for syngas formation in the oxidation zone. Under the experimental conditions, steam and CO2 reforming of methane play only a minor role in syngas formation in the same reaction zone. The contribution of the last two reactions increases with increasing contact time.
Facile One‐Step Catalytic Grafting of N‐Hydroxysuccinimidyl‐Ester‐Functionalized Methallylsilane onto Silica for Enzyme Immobilization
Authors: Jung Un‐Young , Park Jung‐Woo , Han Eun‐Hee , Kang Sin‐Gun , Lee Sera , Jun Chul‐Ho 
Published: January 2011

AbstractA one‐step grafting method of NHS‐ester group is described for the immobilization of enzymes onto a silica surface at room temperature. Pure NHS‐ester functionalized methallylsilane was prepared as a grafting reagent and showed remarkable grafting efficiency with acid catalysts at room temperature. As a result, mesoporous silica balls bearing a covalently immobilized enzyme could be obtained with a high loading rate compared to conventional multistep grafting methods. This one‐step procedure offers the advantages of precise measurement of the high loading efficiency of the NHS‐ester group, and the exclusive formation of only the NHS‐ester group, which reduces the presence of unreacted functionalities.
Optical mapping of DNA: Single‐molecule‐based methods for mapping genomes
Authors: Neely Robert K. , Deen Jochem , Hofkens Johan 
Published: January 2011

AbstractThe technologies associated with DNA sequencing are rapidly evolving. Indeed, single‐molecule DNA sequencing strategies are cheaper and faster than ever before. Despite this progress, every sequencing platform to date relies on reading the genome in small, abstract fragments, typically of less than 1000 bases in length. The overarching aim of the optical map is to complement the information derived from DNA sequencing by providing long‐range context on which these short sequence reads can be built. This is typically done using an enzyme to target and modify at short DNA sequences of, say, six bases in length throughout the genome. By accurately placing these short pieces of sequence on long genomic DNA fragments, up to several millions of bases in length, a scaffold for sequence assembly can be obtained. This review focuses on three enzymatic approaches to optical mapping. Optical mapping was first developed using restriction enzymes to sequence‐specifically cleave DNA that is immobilized on a surface. More recently, nicking enzymes have found application in the sequence‐specific fluorescent labeling of DNA for optical mapping. Such covalent modification allows the DNA to be imaged in solution, and this, in combination with developing nanofluidic technologies, is enabling new high‐throughput approaches to mapping. And, finally, this review will discuss the recent development of mapping with subdiffraction‐limit precision using methyltransferase enzymes to label the DNA with an ultrahigh density. © 2011 Wiley Periodicals, Inc. Biopolymers 95: 298–311, 2011.
Neuronal Differentiation of C17.2 Neural Stem Cells Induced by a Natural Flavonoid, Baicalin
Authors: Li Ming , Tsang Kam‐Sze , Choi Sze‐Ting , Li Karen , Shaw Pang‐Chui , Lau Kwok‐Fai 
Published: January 2011

AbstractNatural medicinal materials are a significant resource for the identification of compounds with specific biological properties. In this study, we employed multipotent C17.2 neural stem cells as a model for screening natural compounds that possess neural differentiation‐inducing properties. We show here for the first time that, out of the 67 compounds tested, the flavonoid baicalin is a potent differentiation‐inducing agent. Baicalin increased the number of cells bearing extended neurites and the expression levels of a number of neuronal markers. Importantly, baicalin promoted the expression of several key neurogenic transcriptional factors. Moreover, we demonstrated that baicalin enhanced the phosphorylation/activation of Erk1/2. Inhibition of Erk1/2 activation by the MEK inhibitor U0126 attenuated the neuronal differentiation‐inducing effect of baicalin. Taken together, our findings suggest that baicalin induces neuronal differentiation of C17.2 neural stem cells and that this is mediated by activation of Erk1/2. Our work lays the foundation for exploring baicalin for the promotion of neural regeneration after injury or disease.
Successful primary percutaneous coronary intervention in the first trimester of pregnancy
Authors: Babic Zdravko , Gabric Ivo Darko , Pintaric Hrvoje 
Published: January 2011

AbstractA 28‐year‐old patient, medical nurse, in 10th week of her second pregnancy suffered ventricular fibrillation just after entering the waiting room of the emergency department. After she was successfully defibrillated, electrocardiography revealed a large acute anteroseptolateral ST elevation myocardial infarction. Urgent coronarography was done (premedication with 300 mg of aspirin and 600 mg of clopidogrel) with 90 min door‐to‐balloon time. Proximal left anterior descending occlusion was found, primary percutaneous coronary intervention was done using Amazonia CroCo 3.0/12 bare‐metal stent, and Thrombolysis in Myocardial Infarction III flow was achieved. During the procedure, the patient was wrapped in lead apron. Because of postresuscitational agitation, procedure was done in intravenous anesthesia. The revealed risk factors were smoking and hypercholesterolemia. PAI‐1 gene 4G/4G genotype and Apo E gene E2/E4 genotype were also found. Estimated X‐ray dosage that fetus received during the procedure was 0.45 mSv, which is less than the upper safe limit in pregnancy. All drugs given to our patient (clopidogrel, aspirin, ivabradine, bisoprolol, anesthetics, low‐molecular‐weight heparin, and unfractionated heparin) have B or C Food and Drug Administration Pregnancy Category. Fetal ultrasonography showed normal fetal growth, and, after consultation with our team, the patient decided to maintain the pregnancy. Before discharge echocardiography showed left ventricle of normal size with anteroseptolateral hypokinesia, small apical aneurysm, left ventricular ejection fraction of 40–45%, and diastolic dysfunction grade II, without pulmonary hypertension. At the 36th week of pregnancy, the patient was hospitalized and closely monitored; clopidogrel and aspirin were discontinued, and low‐molecular‐weight heparin was administered. She gave birth to a normal boy by vaginal delivery with epidural anesthesia and without any complication. © 2010 Wiley‐Liss, Inc.
Sustained long‐term benefit of patent foramen ovale closure on migraine
Authors: Trabattoni Daniela , Fabbiocchi Franco , Montorsi Piero , Galli Stefano , Teruzzi Giovanni , Grancini Luca , Gatto Pamela , Bartorelli Antonio L. 
Published: January 2011

AbstractObjectives: This single‐center, observational, prospective study evaluated the impact of patent foramen ovale (PFO) closure on migraine attacks over time. Background: PFO closure may reduce the frequency and severity of migraine headaches in patients with significant right‐to‐left shunts. Methods: Between May 2000 and September 2009, 305 consecutive patients (mean age, 43 ± 12 years; 54.5% women) with a prior embolic cerebrovascular event underwent PFO closure with the Amplatzer PFO occluder for recurrence prevention. All patients had right‐to‐left shunts; the shunts were associated with migraine symptoms in 77 (25%), either alone (n = 64, 83%) or with aura (n = 13, 17%). Septal aneurysm was present in 15 (19.5%) migraine patients, and 43 (56%) had a previous transient brain ischemic attack. All migraine patients had a computed tomography scan or magnetic resonance imaging, indicating a previous brain ischemic lesion. All 305 patients underwent transthoracic echocardiography with clinical follow‐up at 24 hr, at 3, 6, and 12 months, and then yearly. Results: An acute migraine attack occurred 24–48 hr after PFO closure in 28 (36.4%) of 77 patients. There was a significant reduction (>50%) in the number and intensity of attacks in 46 (60.5%) of 77 patients at the 3‐month follow‐up. At the 12‐month follow‐up, migraine had ceased in 23 (46%) patients, and 20 (40%) had a reduction in the migraine recurrence rate and disabling symptoms. These results were maintained at follow‐up (mean, 28 ± 27 months). There was overall improvement in migraine in 89% of the treated patients. Conclusions: Percutaneous PFO closure in migraineurs may provide beneficial mid‐term and long‐term results, with significant reduction in the intensity and frequency of headache symptoms. © 2010 Wiley‐Liss, Inc.
Coronary obstruction following transcatheter aortic valve‐in‐valve implantation for failed surgical bioprostheses
Authors: Gurvitch Ronen , Cheung Anson , Bedogni Francesco , Webb John G. 
Published: January 2011

AbstractTranscatheter aortic valve implantation (TAVI) for failed surgical bioprostheses, or “valve‐in‐valve” implantation, is a therapeutic option for high‐risk patients. While coronary occlusion during TAVI for native aortic stenosis has been described, in the setting of valve‐in‐valve implantation the bioprsthetic posts may be protective against this complication. We describe the first two cases of coronary occlusion following valve‐in‐valve therapy, both occurring during treatment of degenerated Mitroflow bioprostheses. Aortic root anatomy, coronary ostial position, and the specifics of the bioprosthetic valve type need to be considered in assessing and preventing this rare complication. © 2011 Wiley‐Liss, Inc.
PFO and paradoxical embolism producing events other than stroke
Authors: Dao Catherine N. , Tobis Jonathan M. 
Published: January 2011

AbstractBackground: A patent foramen ovale (PFO) is a risk factor for cerebral events such as cryptogenic stroke, transient ischemic attacks, and migraine headaches. Far less commonly, PFO is associated with non‐cerebral, paradoxical systemic embolic events such as myocardial infarction (MI), renal infarct, and limb ischemia. This report details the incidence of systemic paradoxical emboli at our institution. Methods: 416 patients were referred for evaluation of PFO related conditions from 2001 to 2009. Clinical history and medical records of the patients were reviewed for incidence of cryptogenic stroke, transient ischemic attack (TIA), migraine headache, arterial desaturation, and noncerebral systemic embolism. Results: As the primary presenting symptom, 219 patients had a diagnosis of cryptogenic stroke, 38 patients had migraine headaches, and 80 patients had transient neurologic deficits consistent with a TIA or complex headache. Twelve patients (2.9% of the total population) presented with a presumptive diagnosis of systemic embolism. Eight of these patients had acute MI diagnosed by elevated cardiac biomarkers, electrocardiogram changes, and/or imaging evidence of a left ventricular wall motion abnormality, without evidence of obstructive coronary disease on angiography. Four patients had evidence of peripheral embolism to a systemic artery, including the popliteal artery, ophthalmic artery, and brachial artery. PFO closure was performed in 197 patients (47.4% of the total population), including eight patients in the systemic embolism group. All closure procedures were successful. Conclusion: Although most paradoxical emboli travel to the brain, noncerebral paradoxical embolism is also associated with PFO. In addition to embolism of thrombus, there may be paradoxical passage of vasoactive chemicals that induce intense coronary spasm and myocardial infarction. Diagnosis is often challenging, given the lack of definitive criteria and the need to exclude other potential etiologies. © 2011 Wiley‐Liss, Inc.
Radial access site inflammatory reaction to a recently available hydrophilic coated sheath
Authors: Swaminathan Rajesh V. , Wong S. Chiu 
Published: January 2011

AbstractA sterile inflammatory reaction at the radial access site has been described in the literature as an adverse local reaction to Cook hydrophilic coated sheaths during transradial catheterization. To date, this reaction has not been observed with non‐Cook hydrophilic sheaths. Here, we describe two cases of such a reaction with Glidesheaths™ at our institution. © 2011 Wiley‐Liss, Inc.
Pulmonary perfusion scans following transcatheter patent ductus arteriosus closure using the Amplatzer devices
Authors: Kharouf Rami , Heitschmidt Mary , Hijazi Ziyad M. 
Published: January 2011

AbstractIntroduction: Obstruction to flow in the left pulmonary artery (LPA) is a well‐known complication after transcatheter device closure of patent ductus arteriosus (PDA). This complication has been studied for different devices using lung perfusion radionuclide scintigraphy (LPRS), but not for Amplatzer devices. This study was performed to evaluate the effect of such devices on lung perfusion using LPRS. Methods: This is a retrospective study that looked at all patients who had PDA closure using different Amplatzer devices at our center between July 1999 and January 2007. All patients underwent LPRS within 24 hr of the procedure. We compared LPRS with other hemodynamic data obtained by cardiac catheterization and echocardiography. Results are presented as mean ± SD or median and ranges. Results: A total of 70 patients had PDA closure using an Amplatzer device; median age was 1.8 years (4 months to 75 years) and median weight was 12 kg (5–112 Kg). Nine patients had associated cardiac anomalies. Sixty eight patients had available LPRS. The mean percent of left lung perfusion (LLP) was 42.7% (±6.7%). Excluding patients with pre‐existing LPA stenosis, 17% had abnormally decreased LLP. On hemodynamic measurements, 62 patients had available direct pressure measurements following PDA closure. None had significant increase. No correlation was found with echocardiographic data. Conclusion: PDA closure with Amplatzer family of devices is associated with a relatively significant risk of decreased perfusion to the left lung, mostly mild abnormalities. Comparison with catheterization and echocardiographic measurements showed lack of correlation with LPRS findings.© 2010 Wiley‐Liss, Inc.