bluuebunny/crossref_metadata_2025_split huggingface dataset:
A framework for a brain-derived nosology of psychiatric disorders
Authors: Lett Tristram A. , Vaidya Nilakshi , Jia Tianye , Polemiti Elli , Banaschewski Tobias , Bokde Arun L.W. , Flor Herta , Grigis Antoine , Garavan Hugh , Gowland Penny , Heinz Andreas , Brüh Rüdiger , Martinot Jean-Luc , Martinot Marie-Laure Paillère , Artiges Eric , Nees Frauke , Orfanos Dimitri Papadopoulos , Lemaitre Herve , Paus Tomáš , Poustka Luise , Stringaris Argyris , Waller Lea , Zhang Zuo , Robinson Lauren , Winterer Jeanne , Zhang Yuning , King Sinead , Smolka Michael N. , Whelan Robert , Schmidt Ulrike , Sinclair Julia , Walter Henrik , Feng Jianfeng , Robbins Trevor W. , Desrivières Sylvane , Marquand Andre , Schumann Gunter ,   IMAGEN Consortium,   environMENTAL Consortium
Published: May 2024

AbstractCurrent psychiatric diagnoses are not defined by neurobiological measures which hinders the development of therapies targeting mechanisms underlying mental illness1,2. Research confined to diagnostic boundaries yields heterogeneous biological results, whereas transdiagnostic studies often investigate individual symptoms in isolation. There is currently no paradigm available to comprehensively investigate the relationship between different clinical symptoms, individual disorders, and the underlying neurobiological mechanisms. Here, we propose a framework that groups clinical symptoms derived from ICD-10/DSM-V according to shared brain mechanisms defined by brain structure, function, and connectivity. The reassembly of existing ICD-10/DSM-5 symptoms reveal six cross-diagnostic psychopathology scores related to mania symptoms, depressive symptoms, anxiety symptoms, stress symptoms, eating pathology, and fear symptoms. They were consistently associated with multimodal neuroimaging components in the training sample of young adults aged 23, the independent test sample aged 23, participants aged 14 and 19 years, and in psychiatric patients. The identification of symptom groups of mental illness robustly defined by precisely characterized brain mechanisms enables the development of a psychiatric nosology based upon quantifiable neurobiological measures. As the identified symptom groups align well with existing diagnostic categories, our framework is directly applicable to clinical research and patient care.
Nanopore sequencing reveals that DNA replication compartmentalisation dictates genome stability and instability inTrypanosoma brucei
Authors: Krasiļņikova Marija , Marques Catarina A. , Briggs Emma M. , Lapsley Craig , Hamilton Graham , Beraldi Dario , Crouch Kathryn , McCulloch Richard 
Published: May 2024

AbstractThe genome ofTrypanosoma bruceiis structurally complex. Eleven megabase-sized chromosomes each comprise a transcribed core flanked by silent subtelomeres, housing thousands ofVariant Surface Glycoprotein(VSG) genes. Additionally, silentVSGsare also found on hundreds of sub-megabase chromosomes that harbour 177 bp repeats of unknown function, and multipleVSGtranscription sites localise to the telomeres of both chromosome types. DNA replication dynamics have been described in the megabase chromosome cores but not in the subtelomeres or sub-megabase chromosomes, and targeted early replication of the single activeVSGtranscription site is unexplained. Here, using Nanopore assembly, we mapped DNA replication across this compartmentalised genome. We show that subtelomeres display a paucity of replication initiation events relative to the core, correlating with increased instability in the silentVSGarchive. In addition, early replication of the activeVSGtranscription site is shown to originate from the telomere, likely causing targetedVSGrecombination. Lastly, we demonstrate that the 177 bp repeats act as widespread, conserved DNA replication origins, explaining mitotic stability of the abundant small chromosomes and early DNA replication of megabase chromosome centromeres. Compartmentalized DNA replication dynamics therefore explains howT. bruceibalances stable genome transmission with localised instability driving immune evasion.
PEG-mCherry interactions beyond classical macromolecular crowding
Authors: Haas-Neill Liam , Joron Khalil , Lerner Eitan , Rauscher Sarah 
Published: May 2024

AbstractThe dense cellular environment influences bio-macromolecular structure, dynamics, interactions and function. Despite advancements in understanding protein-crowder interactions, predicting their precise effects on protein structure and function remains challenging. Here, we elucidate the effects of PEG-induced crowding on the fluorescent protein mCherry using molecular dynamics simulations and fluorescence-based experiments. We identify and characterize specific PEG-induced structural and dynamical changes in mCherry. Importantly, we find interactions in which PEG molecules wrap around specific surface-exposed residues in a binding mode previously observed in protein crystal structures. Fluorescence correlation spectroscopy experiments capture PEG-induced changes, including aggregation, suggesting a potential role for the specific PEG-mCherry interactions identified in simulations. Additionally, mCherry fluorescence lifetimes are influenced by PEG and not by the bulkier crowder dextran or by another linear polymer, polyvinyl alcohol, highlighting the importance of crowder-protein soft interactions. This work augments our understanding of macromolecular crowding effects on protein structure and dynamics.
Ionizing radiation has negligible effects on the age, telomere length, and corticosterone levels of Chornobyl tree frogs
Authors: Burraco Pablo , Gabor Caitlin , Bryant Amanda , Gardette Vanessa , Lengagne Thierry , Bonzom Jean-Marc , Orizaola Germán 
Published: May 2024

AbstractPollutants, such as ionizing radiation, released at high levels by human activities can shape ecological and evolutionary processes. The accident occurred at Chornobyl nuclear power plant (Ukraine, April 1986) contaminated a large extension of territory after the deposition of radioactive material. Beyond the immediate negative impact caused by the accident, it is still under debate whether the chronic exposure to the radiation levels currently present in the area signifies a serious threat for organisms. One hypothesis suggests that current levels of radiation may cause unobservable damage in the short-term, but have long-term effects such as decreases in longevity. Here, we investigate through a field-based approach, whether current levels of radiation in Chornobyl negatively impact the age of a semi-aquatic vertebrate, the Eastern tree frogHyla orientalis. We also explore whether radiation induces changes in an ageing marker, telomere length, or in the stress hormone corticosterone. We found no effect of total individual absorbed radiation (including both external and internal exposure) on frog age (n = 197 individuals sampled in three consecutive years). We also did not find any relationship between individual absorbed radiation and telomere length, but a negative relationship between individual absorbed radiation and corticosterone levels. Our results suggest that radiation levels currently experienced by tree frogs in Chornobyl may not be high enough to cause severe chronic damage to semi-aquatic vertebrates such as this species. This is the first study addressing age and stress hormones in Chornobyl wildlife, and thus future research will confirm if these results can be extended to other taxa.
Probiotic Lactobacilli activate Formyl-Peptide Receptor 2
Authors: Dorothee Kretschmer , Ralf Rosenstein , Ahmed Elsherbini , Bernhard Krismer , O’Toole Paul W. , David Gerlach , Andreas Peschel 
Published: May 2024

AbstractChanges in the composition of the human microbiota can negatively impact human health. Probiotic bacteria like many lactobacilli help prevent or repair dysbiosis but it is largely unclear which molecules of these bacteria mediate the probiotic effects. Given the extensive crosstalk between the immune system and microbiome members, we investigated whether lactobacilli activate the formyl-peptide receptor 2 (FPR2), a pattern recognition receptor that is expressed on the surface of intestinal epithelial cells and known to promote wound healing and immune homeostasis.Probiotic strains ofLacticaseibacillus paracasei, Lactiplantibacillus plantarum, andLacticaseibacillus rhamnosuswere isolated from probiotic compounds and sequenced. Calcium influx experiments in FPR1 or FPR2 overexpressing HL60 cells, and primary human neutrophils, along with pharmacological inhibition of FPR2, revealed that culture filtrates of the isolated lactobacilli strongly activate FPR2, promote killing of the methicillin resistantS. aureusUSA300 and induce neutrophil chemotaxis. Pretreatment of culture filtrates with proteinase K reduced FPR2 activity, indicating that the FPR2 ligands are peptides. In silico analysis of the amphipathic properties of the signal peptides of lactic acid bacteria identified selected signal peptides ofL. plantarumwith the ability to predominantly activate FPR2in vitro. Thereby, via targeted activation of FPR2, peptides released by some lactobacilli are likely to positively influence the outcome of inflammatory gut diseases and could be used to treat inflammatory diseases.
Functional plasticity of HCO3-uptake and CO2fixation inCupriavidus necatorH16
Authors: Panich Justin , Toppari Emili , Tejedor-Sanz Sara , Fong Bonnie , Dugan Eli , Chen Yan , Petzold Christopher J. , Zhao Zhiying , Yoshikuni Yasuo , Savage David F. , Singer Steven W. 
Published: May 2024

AbstractUptake and fixation of CO2are central to strategies for CO2-based biomanufacturing.Cupriavidus necatorH16 has emerged as a promising industrial host for this purpose. Despite its prominence, the ability to engineerC. necatorinorganic carbon uptake and fixation is underexplored. Here, we test the role of endogenous and heterologous genes onC. necatorinorganic carbon metabolism. Deletion of one of the four carbonic anhydrases inC. necator, β-carbonic anhydrasecan, had the most deleterious effect onC. necatorautotrophic growth. Replacement of this native uptake system with several classes of dissolved inorganic carbon (DIC) transporters fromCyanobacteriaand chemolithoautotrophic bacteria recovered autotrophic growth and supported higher cell densities compared to wild-type (WT)C. necatorin saturating CO2in batch culture. Several heterologous strains withHalothiobacillus neopolitanusDAB2 (hnDAB2) expressed from the chromosome in combination with diverse rubisco homologs grew in CO2equally or better than the wild-type strain. Our experiments suggest that the primary role of Can carbonic anhydrase during autotrophic growth is for bicarbonate accumulation to support anaplerotic metabolism, and an array of DIC transporters can complement this function. This work demonstrates flexibility in HCO3-uptake and CO2fixation inC. necator, providing new pathways for CO2-based biomanufacturing.Graphical abstract
Female leadership in sports coaching: a case analysis of Chinese coaches through the ability–motivation–opportunity framework
Authors: Gao Qiuling , Zhuang Xiaolin , Li Zijie , Wang Yan 
Published: May 2024

Purpose
This study aims to investigate how the role of female leadership works in sports coaching. Drawing key insights from the ability–motivation–opportunity (AMO) framework of human resource management, the authors explore the underlying mechanisms of female leadership and the role female coaches play in enabling athletes to develop their self-initiative through AMO enhancement.

Design/methodology/approach
The sample in this study is made up of 11female coaches from eight teams in China selected using purposive sampling. Data was collected and analyzed on various aspects of female leadership.

Findings
Analysis of interviews and secondary data show that the mechanisms and enablers related to AMO enhancing practices stimulated by female coaching leadership can increase team performance.

Research limitations/implications
This study opens new avenues for sports science research and strengthens the theoretical and practical understanding of the intentions and mechanisms female coaches implement as leaders in sports coaching.

Originality/value
First, this study contributes to the AMO framework by helping form new theoretical insights based on the understanding of female sports coaching mechanisms. Second, this study provides novel insights into female leadership literature by investigating the mechanisms of female leadership in the context of sports exercise. Third, this study also contributes to the body of research on sports coaching in Asia, especially that of Chinese women in such leadership roles.
Patterns and trends of university-industry research collaboration in Ghana between 2011 and 2020
Authors: Danquah Monica Mensah , Onyancha Omwoyo Bosire , Avuglah Bright Kwaku 
Published: May 2024

Purpose
The ranking of universities and other research-intensive institutions in global ranking systems is based on numerous indicators, including number of articles with external collaboration, number of articles with international collaboration, number of articles with industry collaboration as well as co-patents with industry. The purpose of this paper is to examine university–industry research collaboration in Ghana, with the aim of exploring the relationship between the research output collaborations in the top four universities in Ghana and industry across different geographical scales.

Design/methodology/approach
This study’s data was obtained from the SciVal database, which drawn its data from the Scopus bibliographic and citation database. The bibliographic and citation data were extracted using a search of the publications affiliated to the University of Ghana, for the period 2011–2020.

Findings
Key findings demonstrate a constant rise in the number of research publications by the selected universities over time. Research collaboration intensity in the selected universities in terms of co-authored publications was higher as compared to single-authored publications. University–industry research co-authorships were, however, lower when compared to university–university research co-authorships. The university–industry research co-authorships occurred mostly with Europe, Asia-Pacific and North American-based institutions as opposed to African-based institutions. In Ghana, four industry-based institutions were engaged in intensive research with the selected universities.

Originality/value
This study demonstrates that, for each selected university, it is possible to measure the performance of individual universities in both intra-regional and international collaboration. Such results may be useful in informing policy as well as merit-based public funding of universities in Ghana.
Facilitating corporate sustainability integration: innovation in family firms
Authors: Nguyen Linh H. , Kanbach Dominik K. , Kraus Sascha 
Published: May 2024

PurposeThe purpose of the study is to understand the relationship between family-driven innovation and the incorporation of corporate sustainability in German family firms.Design/methodology/approachThe study conducted 26 interviews with 22 German family firms. Thematic analysis was undertaken on the collected data resulting in five major themes.FindingsThe study identified five main themes of corporate sustainability-oriented innovation in family firms, which include measuring corporate sustainability performances, building corporate sustainability-oriented infrastructure, stabilizing/optimizing operations, enhancing operational flexibility/independence and knowledge management and development. The study also provides an activity-based guide for family firms to use innovation to achieve corporate sustainability goals and present the findings’ implications for policymakers.Originality/valueThe present study is the first study to empirically investigate the relationship between family-driven innovation and the incorporation of corporate sustainability at each of the corporate sustainability maturity levels.
Real‐world efficacy and safety of BRAF‐targeted therapy for patients with advanced melanoma: A single‐center retrospective study in Japan
Authors: Nakano Eiji , Takahashi Akira , Ogata Dai , Namikawa Kenjiro , Yamazaki Naoya 
Published: May 2024

AbstractMost clinical trials investigating targeted therapies for patients harboring BRAF V600 mutations have included mostly White patients, and data for Asian patients are scarce. Although there are several retrospective studies in Japanese patients, they have investigated only the dabrafenib + trametinib regimen, and have had a short follow‐up period. We conducted a single‐center retrospective study to update previous studies and compare the outcomes with those in White patients. We analyzed 89 patients who received dabrafenib + trametinib or encorafenib + binimetinib, including 11 who received both treatment regimens. The overall response rate was 79.8%, with complete response in 25 patients (28.1%) and partial response in 45 patients (51.7%). The median progression‐free survival was 13.7 months, and the median overall survival was 32.9 months. The 3‐year progression‐free and overall survival rates were 31.8% and 47.9%, respectively. Although the two regimens showed no significant differences in efficacy, their safety profiles differed, as reported in clinical trials. Therefore, the most frequent adverse event associated with the dabrafenib + trametinib regimen was pyrexia (61.3%) and that of encorafenib + binimetinib was blurred vision (32.0%). Switching directly to another targeted therapy after progressive disease showed no clinical response; however, rechallenge followed by immune checkpoint inhibitor therapy showed a certain response. As a prognostic factor, performance status was associated with progression‐free survival, and performance status, serum lactate dehydrogenase level, and dose interruption were associated with overall survival in the multivariate analysis. Real‐world data on targeted therapy for patients with melanoma in Japan suggest that both dabrafenib + trametinib and encorafenib + binimetinib show similar efficacy and safety in Asian and White patients.
Predicting regional cumulative effects of future development on coastal ecosystems to support Indigenous governance
Authors: Tulloch Vivitskaia J. D. , Adams Megan , Finn Riley , Bourbonnais Mathieu , Avery‐Gomm Stephanie , Penn Briony , Martin Tara G. 
Published: May 2024

Abstract

To achieve better biodiversity outcomes and match local governance capacity, cumulative effects assessment frameworks that combine Indigenous and western knowledge to predict future development impacts on biodiversity are needed.

We developed a spatial future‐focused model informed by inclusive elicitation and strategic foresight to assess the regional cumulative effects of development on ecosystem health across the land and sea. We collaborated with three First Nations on the Central Coast of British Columbia, Canada, enabling Indigenous priorities, knowledge and values to drive the process, from the choice of priority ecosystem components (including salmon, herring, seabirds and bears), to identifying future development scenarios (based on forestry, energy/mining, tourism and salmon aquaculture sectors). Bayesian networks were populated with empirical data and expert judgement elicited from knowledge holders to predict the cumulative effects of current and future pressures on species and ecosystems.

Under current conditions, the lowest probability of persistence was predicted for Pacific salmon (37%), followed by Pacific herring (43%). Under future conditions, the greatest declines in species health were associated with the intense development of mining, tourism and forestry, with up to a 54% decline from the current baseline health estimates predicted for Marbled Murrelets and old‐growth forest.

Future outcomes for overall ecosystem health were predicted to be worst in scenarios with high future forestry activities (>60% decline in some areas). The continuation or development of all four industries resulted in an 8% decline overall in ecosystem health across the Central Coast. In contrast, predicted ecosystem health in the tourism economy scenario increased up to 15% in some marine areas, primarily driven by the removal of salmon aquaculture and forestry activities.

Synthesis and applications. Our study demonstrates an inclusive, regional approach to assessing the cumulative effects of future development on coastal species. The novel participatory tools and predictive framework draw upon and interweave multiple forms of knowledge, enabling Indigenous values to drive the process, and appropriately integrate Indigenous knowledge into regional cumulative effects assessment. Our interactive web application provides First Nations partners access to all outputs, supporting Indigenous‐led governance and in situ ecosystem‐based management of their lands and water.
Another look at energy consumption and environmental sustainability target through the lens of the load capacity factor: Accessing evidence from MINT economies
Authors: Bekun Festus Victor , Uzuner Gizem , Meo Muhammad Saeed , Yadav Ashutosh 
Published: May 2024

AbstractThe relationship between energy utilization and the environment is crucial in an era of environmental concerns by global economies and rising energy consumption. Emerging economies such as Mexico, Indonesia, Nigeria, and Turkey (hereafter, MINT) face complex trade‐offs between economic growth and environmental sustainability. Strengthening this study are the UN Sustainable Development Goals prepositions on access to clean and alternative energy, decent economic growth, responsible production and consumption and climate action (UN‐SDGs‐7, 8, 12, and 13). The present study examines the environmental Kuznets curve (EKC) hypothesis for MINT economies within the framework of the load capacity factor (LCF). The article leverages panel econometrics to operationalize the relationship between study variables. Empirical findings show that the present study fails to confirm the presence of EKC. Thus, it implies that the MINT economies are at their first stage of accelerated economic growth which might result in an augmented ecological footprint and exert pressure on natural resources, as indicated by the observed negative outcome. Furthermore, there is a positive and significant relationship between renewable energy consumption (RENENG) and LCF. It implies that a 1% increase in RENENG leads to an increase in LCF of 0.70%. These outcomes indicate that the level of RENENG in MINT economies is not sufficient to mitigate climate change issues. Thus, from a policy perspective, there is a need for change in the MINT nations' energy portfolio mix, such as the need to switch from conventional energy sources (fossil fuels) to renewable energy sources, including solar, wind, photovoltaic and hydropower, which usually have a smaller negative impact on the environment. Furthermore, there is a need for investment in new and green energy technologies in the countries investigated to arrive at a clean and better ecosystem as desired. More insight is outlined in the concluding section.