bluuebunny/crossref_metadata_2025_split huggingface dataset:
PELATIHAN PEMBUATAN MULTIMEDIA INTERAKTIF BAGI KELOMPOK SIM PKB GURU SD
Authors: Febriandi Riduan , Aswarliansyah Aswarliansyah 
Published: May 2024

Pelatihan Pembuatan Multimedia Interaktif untuk pembelajaran daring bagi kelompok Sistem Informasi Manajemen untuk Pengembangan Keprofesian yang Berkelanjutan (SIM PKB) Guru SD kecamatan Muara Beliti adalah strategi yang tepat untuk mewujudkan tujuan pendidikan pada masa pembelajaran daring. Multimedia Interaktif adalah suatu kumpulan dari beberapa media seperti teks, gambar, audio, video, dan animasi yang bersifat interaktif atau yang dapat dioperasikan oleh pengguna sehingga pengguna dapat memilih apa yang diinginkan untuk proses selanjutnya. Dengan menggunakan multimedia interaktif siswa akan lebih mudah memahami materi yang diberikan oleh guru, dan guru jadi lebih efesien dan efektif memberikan pelajaran kepada siswa SD. Setelah dilakukan pengabdian Pelatihan Pembuatan Multimedia Interaktif untuk pembelajaran daring dalam meningkatkan keterampilan mengajar guru SD se Kecamatan Muara Beliti, akan diperoleh luaran sebuah multimedia interaktif yang dapat digunakan oleh beberapa sekolah dan beberapa pelajaran secara daring dalam menerapkan pemebelajaran tematik di tingkat SD. Untuk mendapatkan hasil yang maksimal pengabdian dilakukan secara berkala dengan melakukan pemetaan jumlah guru, sarana dan prasarana yang sesuai, dan waktu pelaksanaan dilakukan 2 kali pelatihan atau 2 minggu. Setelah pelatihan selesai akan dilakukan evaluasi berupa pendampingan sehingga para guru yang mengikuti pelatihan dapat membuat bahan ajar masing-masing.
PENDAMPINGAN PEMBUATAN PERANGKAT PEMBELAJARAN KURIKULUM MERDEKA DAN REDESIGN RUANG PERPUSTAKAAN DI SD NURUL MUTTAQIN KABUPATEN MALANG
Authors: Rawanoko Endrise Septina , Oktaviansyah Aulia Rahman , Y.P Hariyono Saputra 
Published: May 2024

Guru merupakan seorang tenaga pendidik yang memiliki tugas mendidik, mengajar, membimbing, memberikan penilaian dan melakukan evaluasi pada peserta didik. Setiap pendidik pada satuan pendidikan berkewajiban menyusun RPP secara lengkap agar meningkatkan hasil proses belajaran mengajar. Kegiatan ini melibatkan kerjasama dengan sekolah mitra. Terdapat satu sekolah yang terlibat dalam kegiatan ini, yaitu SD Islam Nurul Muttaqin Kabupaten Malang. Untuk menyukseskan kemitraan ini, guru yang akan menjadi peserta pelatihan perlu mendapatkan penguatan konsep materi Kurikulum Merdeka, dan penyusunan RPP. Metode pelaksanaan kegiatan ini yaitu workshop pelatihan, pendampingan dan impelementasi Penyusunan RPP Kurikulum Merdeka. Pada akhir kegiatan ini, guru akan mengimplementasikan RPP yang telah disusun sesuai kelasnya. Dalam pendesiminasian RPP ini, tim akan mendampingi dan melakukan observasi. Dari Hasil observasi ini yang akan dijadikan bahan Refleksi Bersama. Dapat disimpulkan bahwa guru telah menerapkan Kurikulum Merdeka dengan baik. Hal ini dapat diketahui dari RPP yang dirancang dan kegiatan pembelajaran. Pada permasalahan mitra kedua yaitu terkait ruang perpustakaan maka tim akan membuatkan design perpustakaan yang mumpuni pada peserta didik tingkat sekolah dasar.
The role of tolvaptan add-on therapy in patients with acute heart failure: a systematic review and network meta-analysis
Authors: Pratama Vireza , Budiono Jordan , Thobari Jarir At , Widyantoro Bambang , Anggraeni Vita Yanti , Dinarti Lucia Kris 
Published: May 2024

BackgroundSeveral conflicting reviews have concluded that the use of loop diuretics is associated with poorer clinical and safety outcomes. Therefore, this study aimed to investigate the efficacy and safety of tolvaptan as an adjunct to conventional diuretic therapy in patients with acute heart failure (AHF).MethodsA comprehensive search was conducted on PubMed, Embase, ProQuest, EBSCO, and Cochrane Library until 24 May 2023 to identify randomized controlled trials that compared the effects of tolvaptan with conventional therapy and placebo in patients with AHF. The quality assessment of the included trials was conducted using the Cochrane risk of bias. A network meta-analysis (NMA) was conducted to examine the dosage effect of tolvaptan.ResultA total of 17 studies with 18 reports, involving 10,039 patients, were selected. The tolvaptan add-on therapy significantly alleviated dyspnea [24 h: RR 1.16 (1.04, 1.29), 48 h: RR 1.18 (1.04, 1.33)], reduced body weight within 48 h [Asian group, MD −0.93 (−1.48, −0.38); non-Asian group, MD −2.76 (−2.88, −2.65)], reduced edema [RR 1.08 (1.02, 1.15)], increased serum sodium [non-Asian group, MD 3.40 (3.02, 3.78)], and resulted in a change in serum creatinine [MD −0.10 (−0.18, −0.01)]. No significant differences were observed in mortality and rehospitalization. The NMA suggested that an intermediate dosage (15 mg/day) might offer the best efficacy in reducing dyspnea within 24 h, reducing edema, increasing serum sodium, and lowering the incidence of worsening renal function (WRF).ConclusionIn conclusion, the meta-analysis showed that tolvaptan contributed to the short-term alleviation of congestive symptoms, elevated sodium levels, and a lower incidence of WRF. However, no significant benefits were observed in long-term symptoms, rehospitalization rates, and mortality. An intermediate dosage of tolvaptan might be considered the optimal choice for various clinical outcomes.Systematic Review Registrationhttps://www.crd.york.ac.uk/, PROSPERO (CRD42023420288).
Comparative analysis and implementation of DC microgrid systems versus AC microgrid performance
Authors: Memon Danish Asad , Ali Khawaja Haider , Memon Abdul Aziz , Ansari Jamshed Ahmed , Badar Jahangeer , Alharbi Mohammed , Banatwala Ali Zain , Kumar Manoj 
Published: May 2024

DC power systems have emerged as a cost-effective solution for electric power generation and transmission, challenging the dominance of AC distribution systems. However, a comprehensive efficiency comparison between DC and AC microgrids remains understudied. This study seeks to explore and conduct a thorough survey on development and designing of DC microgrids to address this gap. Firstly, a comprehensive literature review comparing the efficiencies of AC and DC microgrids has been presented. The analysis highlights the superior efficiency of DC distribution systems over AC systems, supported by detailed advantages. Secondly, hardware implementation has been performed to directly compare the efficiency of DC versus AC systems. Research validity and application are further improved by the hardware prototype’s scalability, which in simulation allows for a thorough assessment of system stability over a range of scenarios from four to six terminals. Test results from the built hardware prototype demonstrate an astounding 15% increase in efficiency using the DC system compared to the AC system, demonstrating its potential for improved performance in real-world scenarios. In simulation results, the designed DC microgrid demonstrates stable voltages of 500V under steady state operation and rapid recovery within 80 ms under both symmetrical and asymmetrical faults has been observed. The research being investigated utilizes hardware implementation and simulation to provide useful insights into the efficiency and stability of DC microgrids in comparison to AC systems. These results are important for developing robust power distribution networks in modern energy environments, promoting sustainability and dependability in infrastructure growth.
Effect of clinical whole exome sequencing in aetiological investigation and reproductive risk prediction for a couple with monogenic inherited diseases
Authors: Wang Yanan , Chai Yuqiong , Wang Jieqiong , Gao Mingya , Zang Weiwei , Chang Yujie 
Published: May 2024

ObjectiveTo determine the genetic causes of monogenic inherited diseases in a couple using clinical whole exome sequencing (WES) and advise on their reproductive choices.MethodsWES was applied to a couple seeking reproductive advice, the female with short stature and the male with congenital cataracts.Results(1) The woman exhibited a 13.8 Kb heterozygous deletion at chrX: 591590–605428 (hg19). This region corresponds to exons 2–6 of the short-stature homeobox-containing (SHOX) gene (NM000451). Associated diseases involving the SHOX gene range from severe Leri–Weill dyschondrosteosis to mild nonspecific short stature. Meanwhile, further validation using a quantitative reverse transcription polymerase chain reaction assay confirmed the heterozygous deletion of the SHOX gene in the proband, as well as other family members with similar clinical characteristics (the proband’s mother, aunt, and cousin). Multiple pathogenic reports of this variant have been included in the HGMD database. Per the American College of Medical Genetics and Genomics (ACMG) classification criteria, this deletion is classified as pathogenic. (2) For the male patient, a heterozygous variant was detected in the CRYBB3 gene: NM004076: c.226G&gt;A (p.Gly76R). Variants in the CRYBB3 gene can cause Cataract 22 (OMIM: 609741). At present, this variant locus is not included in databases such as the gnomAD, while both SIFT and PolyPhen2 deem this locus ‘damaging’. Moreover, further validation by Sanger sequencing confirmed that the variant was inherited from the male patient’s mother, who also had cataracts. According to ACMG standards and guidelines, the c.226G&gt;A (p.Gly76Arg) variant in the CRYBB3 gene is classified as having ‘uncertain significance’.ConclusionWES identified pathogenic variants in both individuals, suggesting a 25% chance of a healthy child naturally. Third-generation assisted reproductive techniques are recommended to minimize the risk of affected offspring.
Status and long-term changes of coral reefs around Zanzibar
Authors: Ussi Ali M. , Mohammed Mohammed S. , Rashid Rashid J. , Sheikh Mohammed A. , Staehr Peter A. , Muhando Christopher A. , Yahya Saleh , Dahl Karsten 
Published: May 2024

IntroductionCoral reefs as key ecosystems in Zanzibar are exposed to several anthropogenic and natural stressors.MethodsThe benthic composition and coverage of coral reefs were investigated on three data sets involving ten coral reefs monitored from 1992 to 2016. Firstly, we investigated differences in the reef composition using data from seven reefs in 2015. Secondly, we analyzed communities on three distinctive reefs (2010 to 2012) to understand the importance of seasons and reef zones (slope, crest and flat) on species abundance. Finally, we investigated long-term changes (1992 to 2016) of five reefs.ResultsBranching Porites and Acropora, and soft coral order Corallimorpharia, characterized sheltered reef communities. Soft corals and algal species characterized the reef communities exposed to strong hydrodynamic conditions, which also displayed greater cover of rocks and rubbles. The average dissimilarity between reefs ranged between 60% and 75%. The seasonal changes in community structure for reefs near Stone Town were mostly associated with soft coral Corallimorpharia. Indeed, the bare rock and algae distinguished the northern exposed reef from more sheltered reefs. Acropora was a key genus for the sheltered Chumbe reef, which explained between 14% and 18% of the dissimilarities among the three reefs. Hard corals covered between 40% and 70% in most years, with severe declines following El Niño events in 1998 and 2016. The dominating genus Acropora showed a strong decline from the late 1990s’ with signs of recovery at remote reefs compared to reefs closer to human residence.DiscussionOur results highlight the importance of seasonality and spatial differences, reflecting differences in human impact and physical exposure and significant long-term changes in coral communities. Continued monitoring of reef health is essential to evaluate the success of ongoing management to sustain the reef services.
Production and characterization of rhamnolipid biosurfactant from thermophilic Geobacillus stearothermophilus bacterium isolated from Uhud mountain
Authors: Albasri Hibah M. , Almohammadi Asmaa A. , Alhhazmi Areej , Bukhari Duaa A. , Waznah Moayad S. , Mawad Asmaa M. M. 
Published: May 2024

IntroductionBiosurfactants have been given considerable attention as they are potential candidates for several biotechnological applications.Materials and methodsIn this study, a promising thermophilic biosurfactant-producing HA-2 was isolated from the volcanic and arid region of Uhud mountain, Madinah, Saudi Arabia. It was identified using 16S rRNA gene sequence analysis. The biosurfactant production ability was screened using different methods such as the drop collapse test, oil spreading test, hemolytic activity test, CTAB test, and emulsification index. The ability of rhamnolipid production by the tested strain was confirmed by the polymerase chain reaction (PCR) of rhlAB. The affinity of thermophilic HA-2 to hydrophobic substrates was also investigated. Optimization of biosurfactant production was conducted. The biological activities of produced surfactant were investigated.Results and discussionThe isolated HA-1 was identified as Geobacillus stearothermophilus strain OR911984. It could utilize waste sunflower frying oil (WSFF) oil as a low-cost carbon source. It showed high emulsification activity (52 ± 0.0%) and positive results toward other biosurfactant screening tests. The strain showed high cell adhesion to hexane with 41.2% cell surface hydrophobicity. Fourier-transform infrared (FTIR) spectra indicated the presence of hydrophobic chains that comprise lipids, sugars, and hydrophilic glycolipid components. The optimization results showed the optimal factors included potato peel as a carbon source with 68.8% emulsification activity, yeast extract as a nitrogen source with 60% emulsification activity, a pH of 9 (56.6%), and a temperature of 50° (72%). The kinetics showed that optimum biosurfactant production (572.4 mg/L) was recorded at 5 days of incubation. The produced rhamnolipid biosurfactant showed high antimicrobial activity against some human and plant pathogenic bacterial and fungal isolates and high antioxidant activity (90.4%). In addition, it enhanced wheat (Triticum aestivum) growth, with the greatest enhancement obtained with the 5% concentration. Therefore, thermophilic G. stearothermophilus is a promising rhamnolipid biosurfactant producer that utilizes many organic wastes. The produced biosurfactant could be applied as a promising emulsifier, antimicrobial, antioxidant, and plant growth promoter.
Investigating the antimicrobial and anti-inflammatory effects of Lactobacillus and Bifidobacterium spp. on cariogenic and periodontitis pathogens
Authors: Mahdizade Ari Marzie , Mirkalantari Shiva , Darban-Sarokhalil Davood , Darbandi Atieh , Razavi Shabnam , Talebi Malihe 
Published: May 2024

BackgroundThe use of probiotics is emerging as an innovative approach to managing oral health issues and mediating the immune system. The current study assessed the in vitro impacts of non-orally isolated probiotics on periodontitis and tooth decay pathogens.MethodsBriefly, the persistence of probiotics in exposure to oral cavity enzymes, hydrogen peroxide, and saliva samples was examined. It was also investigated the biofilm formation and aggregation ability of probiotics, the adherence of probiotics in human gingival fibroblast cell (HGFC) lines and molar teeth samples, and the potential of probiotics to co-aggregate with oral pathogens. Additionally, the current study evaluated the effects of live probiotics on virulence gene expression, biofilm production of main oral pathogens, and changes in inflammation markers.ResultsThe probiotics remained alive when exposed to enzymes in the oral cavity, hydrogen peroxide, and saliva at baseline, 1, 3, and 5 h after incubation at 37°C (p-value &lt;0.05). Probiotics demonstrated to produce biofilm and aggregation, as well as adherence to HGFCs and maxillary molars (p-value &gt;0.05). They showed significant co-aggregation with oral pathogens, which were recorded as 65.57% for B. bifidum 1001 with S. mutans, 50.06% for B. bifidum 1005 with P. gingivalis, 35.6% for L. plantarum 156 with F. nucleatum, and 18.7% for B. longum 1044 with A. actinomycetemcomitans after 8 h of incubation. A balance between pro-inflammatory and anti-inflammatory cytokines, along with inhibition of biofilm formation and changes in virulence gene transcripts, were observed. However, most of these changes were not statistically significant (p-value &gt;0.05).ConclusionThis study demonstrated the direct link between adhesiveness, aggregation, and biofilm formation with probiotic antibacterial activity. In addition to the careful selection of suitable probiotic strains, the concentration and origin of probiotic isolates should be considered.
The causal role of gut microbiota in susceptibility of Long COVID: a Mendelian randomization study
Authors: Li Zuming , Xia Qinghua , Feng Jieni , Chen Xueru , Wang Yushi , Ren Xiaolei , Wu Siyi , Yang Rongyuan , Li Jiqiang , Liu Yuntao , Lu Yue , Chen Jiankun 
Published: May 2024

BackgroundLong COVID is a major challenge facing the public. Gut microbiota is closely related to Long COVID. However, the causal effects between gut microbiota and Long COVID remains unclear.MethodsUsing summary statistics from Genome-Wide Association Studies (GWAS), Mendelian randomization (MR) analyses were performed to investigate the relationship between gut microbiota and Long COVID. The primary statistical method employed was Inverse Variance Weighted (IVW). Sensitivity analyses were then conducted to evaluate the reliability of the findings and account for potential confounding variables. Finally, a reverse MR analysis was conducted to examine potential associations between Long COVID and genetically predicted gut microbiota compositions.ResultsThere were 2 positive and 1 negative causal effect between gut microbiota and Long COVID. Meta-analysis results show that genus Parasutterella (OR = 1.145, 95%CI = 1.035 ∼ 1.266, P = 0.008) and genus Oscillospira (OR = 1.425, 95%CI = 1.235 ∼ 1.645, P &lt; 0.001) significantly increased the risk of Long COVID. And genus Eisenbergiella (OR = 0.861, 95%CI = 0.785 ∼ 0.943, P = 0.001) significantly decreased the risk of Long COVID. Neither the pleiotropy nor the heterogeneity was observed. Reverse causal effect does not hold.ConclusionOur research has provided genetic evidence that establishes multiple causal relationships between the gut microbiota and Long COVID, supporting the role of the gut microbiota in Long COVID. It is possible that different taxa play a role in the development of Long COVID. The causal relationships identified in this study require further investigation.
A deep learning method for classification of HNSCC and HPV patients using single-cell transcriptomics
Authors: Jarwal Akanksha , Dhall Anjali , Arora Akanksha , Patiyal Sumeet , Srivastava Aman , Raghava Gajendra P. S. 
Published: May 2024

BackgroundHead and Neck Squamous Cell Carcinoma (HNSCC) is the seventh most highly prevalent cancer type worldwide. Early detection of HNSCC is one of the important challenges in managing the treatment of the cancer patients. Existing techniques for detecting HNSCC are costly, expensive, and invasive in nature.MethodsIn this study, we aimed to address this issue by developing classification models using machine learning and deep learning techniques, focusing on single-cell transcriptomics to distinguish between HNSCC and normal samples. Furthermore, we built models to classify HNSCC samples into HPV-positive (HPV+) and HPV-negative (HPV−) categories. In this study, we have used GSE181919 dataset, we have extracted 20 primary cancer (HNSCC) samples, and 9 normal tissues samples. The primary cancer samples contained 13 HPV− and 7 HPV+ samples. The models developed in this study have been trained on 80% of the dataset and validated on the remaining 20%. To develop an efficient model, we performed feature selection using mRMR method to shortlist a small number of genes from a plethora of genes. We also performed Gene Ontology (GO) enrichment analysis on the 100 shortlisted genes.ResultsArtificial Neural Network based model trained on 100 genes outperformed the other classifiers with an AUROC of 0.91 for HNSCC classification for the validation set. The same algorithm achieved an AUROC of 0.83 for the classification of HPV+ and HPV− patients on the validation set. In GO enrichment analysis, it was found that most genes were involved in binding and catalytic activities.ConclusionA software package has been developed in Python which allows users to identify HNSCC in patients along with their HPV status. It is available at https://webs.iiitd.edu.in/raghava/hnscpred/.
Wrist-ankle acupuncture for primary dysmenorrhea: a randomized controlled trial evaluating the efficacy of an analgesic strap
Authors: Zhai Shujie , Wang Chenmiao , Ruan Yi , Liu Yue , Ma Rui , Fang Fanfu , Zhou Qinghui 
Published: May 2024

BackgroundDrawing on the principles of wrist-ankle acupuncture (WAA), our research team has developed a portable device for WAA point compression, termed the acupressure wrist-ankle strap (AWA). The current study aims to evaluate the efficacy of the AWA in alleviating pain associated with primary dysmenorrhea.MethodsA single-blind, randomized clinical trial was conducted from April 1, 2019, to December 31, 2019. 78 participants with primary dysmenorrhea were recruited from Shanghai University of Traditional Chinese Medicine. All participants were treated on the first day of menstruation for 30 min. Participants in the AWA group used the AWA, the internal side of which is equipped with a tip compression component, while participants in the non-acupressure wrist-ankle acupuncture(NAWA)group used the NAWA, with the inside tip pressing parts removed. The main outcome was the difference in visual analogue scale (VAS) score between baseline and 30 minutes after randomization.ResultsA total of 78 participants aged 18 to 30 years were included in the intention-to-treat analyses. The VAS scores (mean [standard deviation]) in the AWA group were significantly lower than those in the NAWA group at each time point of intervention (5 minutes: 95% CI, [−1.27 to −0.68], p &lt; 0.001; 10 minutes: 95% CI, [−2.34 to −1.51], p &lt; 0.001; 30 minutes: 95% CI, [−3.74 to −2.72], p &lt; 0.001). In the AWA group, 16 participants reported “obvious relief” of dysmenorrhea pain while 23 did not; the average onset time of analgesia they reported were (21.50 ± 3.65) min, while no subjects in NAWA group reported obvious pain relief. The pain threshold (mean [standard deviation]) at SP9 of both sides in AWA group decreased significantly after intervention that in NAWA group (Left: 95% CI, [−5.02 to −1.81], p &lt; 0.001; Right: 95% CI, [−7.67 to −4.24], p &lt; 0.001). There was no significant change in the temperature at CV4 in either group (95% CI, [−0.63 to −0.66], p = 0.970).ConclusionThis trial substantiates our hypothesis that the AWA provides immediate analgesic effects. The AWA represents an effective and safe non-invasive physical therapy option, which patients can self-administer to alleviate abdominal pain