bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improving the blood compatibility of material surfaces via biomolecule‐immobilized mussel‐inspired coatings
Authors: Wei Qiang , Li Beijia , Yi Nan , Su Baihai , Yin Zehua , Zhang Fulong , Li Jie , Zhao Changsheng 
Published: October 2010

AbstractIn this article, we presented a general protocol to prepare biomolecule‐immobilized mussel‐inspired polydopamine (PDA) coatings to improve the blood compatibility of broad ranges of material surfaces. It needs only a simple immersion of substrates in dopamine solution at alkaline pH to form mussel‐inspired PDA coating, and then immersing the PDA coated substrates into biomolecule solution to conjugate biomolecules. XPS, water contact angle analysis, and protein assay confirmed that biomolecules could be successfully coated on several material surfaces, including nylon, cellulose, and polyethersulfone membrane surfaces. For the protein fouling resistance, the bovine serum albumin (BSA) modified surfaces were more effective than the amino acid modified surfaces. And the platelet adhesion on the BSA‐modified material surfaces was obviously depressed. These results indicated that the blood compatibility of the surfaces was improved by the biomacromolecule‐immobilized mussel‐inspired coating which might be considered as a universal coating to modify a wide variety of materials. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Frameshift mutations in dentin phosphoprotein and dependence of dentin disease phenotype on mutation location
Authors: Nieminen Pekka , Papagiannoulis-Lascarides Lisa , Waltimo-Siren Janna , Ollila Päivi , Karjalainen Sara , Arte Sirpa , Veerkamp Jaap , Tallon Walton Victoria , Chimenos Küstner Eduard , Siltanen Tarja , Holappa Heidi , Lukinmaa Pirjo-Liisa , Alaluusua Satu 
Published: October 2010

Abstract
We describe results from a mutational analysis of the region of the dentin sialophosphoprotein (DSPP) gene encoding dentin phosphoprotein (DPP) in 12 families with dominantly inherited dentin diseases. In eight families (five mutations in the N-terminal third of DPP), the clinical and radiologic features were uniform and compatible with dentin dysplasia type II (DD-II) with major clinical signs in the deciduous dentition. In the other families (four mutations in the more C-terminal part), the permanent teeth also were affected, and the diseases could be classified as variants of dentinogenesis imperfecta. Attrition was not prominent, but periapical infections were common. Discoloring with varying intensity was evident, and pulps and root canals were obliterated in the permanent dentition. All mutations caused a frameshift that replaced the Ser-Ser-Asx repeat by a code for a hydrophobic downstream sequence of approximately original length. We conclude that frameshift mutations in DSPP explain a significant part of dentin diseases. Furthermore, we propose that the location of the mutation is reflected in the phenotypic features as a gradient from DD-II to more severe disease that does not conform to the classic definitions of DI-II. © 2011 American Society for Bone and Mineral Research.
Electronic and vibrational linear and nonlinear polarizabilities of Li@C60 and [Li@C60]+
Authors: Reis H. , Loboda O. , Avramopoulos A. , Papadopoulos M. G. , Kirtman B. , Luis J. M. , Zaleśny R. 
Published: October 2010

AbstractElectronic and vibrational nuclear relaxation (NR) contributions to the dipole (hyper)polarizabilities of the endohedral fullerene Li@C60 and its monovalent cation [Li@C60]+ are calculated at the (U)B3LYP level. Many results are new, while others differ significantly from those reported previously using more approximate methods. The properties are compared with those of the corresponding hypothetical noninteracting systems with a valence electron transferred from Li to the cage. Whereas the NR contribution to the static linear polarizabilities is small in comparison with the corresponding electronic property, the opposite is true for the static hyperpolarizabilities. A relatively small, but non‐negligible, NR contribution to the dc‐Pockels effect is obtained in the infinite frequency approximation. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2011
Crystal molecular dynamics simulations to speed up MM/PB(GB)SA evaluation of binding free energies of di‐mannose deoxy analogs with P51G‐m4‐Cyanovirin‐N
Authors: Vorontsov Ivan I. , Miyashita Osamu 
Published: October 2010

AbstractComplexes of two Cyanovirin‐N (CVN) mutants, m4‐CVN and P51G‐m4‐CVN, with deoxy di‐mannose analogs were employed as models to generate conformational ensembles using explicit water Molecular Dynamics (MD) simulations in solution and in crystal environment. The results were utilized for evaluation of binding free energies with the molecular mechanics Poisson‐Boltzmann (or Generalized Born) surface area, MM/PB(GB)SA, methods. The calculations provided the ranking of deoxy di‐mannose ligands affinity in agreement with available qualitative experimental evidences. This confirms the importance of the hydrogen‐bond network between di‐mannose 3′‐ and 4′‐hydroxyl groups and the protein binding site BM as a basis of the CVN activity as an effective HIV fusion inhibitor. Comparison of binding free energies averaged over snapshots from the solution and crystal simulations showed high promises in the use of the crystal matrix for acceleration of the conformational ensemble generation, the most time consuming step in MM/PB(GB)SA approach. Correlation between energy values based on solution versus crystal ensembles is 0.95 for both MM/PBSA and MM/GBSA methods. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2011
Activated carbons prepared from biogas residue: characterization and methylene blue adsorption capacity
Authors: Yuan Xianzheng , Shi Xiaoshuang , Zeng Shujuan , Wei Yueli 
Published: October 2010

AbstractBACKGROUND: In China, some biogas residue, which cannot be utilized by microbes in the anaerobic process, has been used as fertilizer. More has been deposited in biogas plants or on land around the plants. This has an effect on the environmental protection of the biogas plant, especially if the high lignin content in the biogas residue is not handled properly.RESULTS: In this study biogas residue has been used for the preparation of activated carbons by phosphoric acid activation. Textural characterization and feasibility of employing the prepared activated carbon to remove methylene blue (MB) from aqueous solution were investigated. The results show that the activated carbons have high surface area (1950 m2 g−1) and pore volume (1.232 cm3 g−1). Equilibrium data were best described by the Langmuir isotherm model, with a maximum monolayer adsorption capacity of 344.83 mg g−1 at 25 °C. Among the kinetic models studied, the pseudo‐first‐order model was found to be the most applicable to describe the adsorption of MB.CONCLUSIONS: The adsorption performance of activated carbons prepared from biogas residues (BR‐AC) was comparable with that of commercial material and other adsorbents reported in earlier studies and presents a high value added application for biogas residues. Copyright © 2010 Society of Chemical Industry
The diagnosis of anomalous origin of the left coronary artery from the pulmonary artery by echocardiography: Case report
Authors: Huang He , Zhang Ju‐qian , Li Chen , Rao Li , Feng Yuan , Dian Ke , Tang Hong 
Published: October 2010

AbstractThe anomalous origin of the left coronary artery from the pulmonary artery is a rare congenital heart disease that affects 1 of 300,000 live births. We present the case of an 18‐year‐old female presenting with chest pain and dyspnea after vigorous exercise, and whose two‐dimensional echocardiogram initially displayed a “normal connection” between the left coronary artery with the aortic sinus of Valsalva. Using a systematic diagnostic echocardiography approach, we rightfully diagnosed it as anomalous origin of the left coronary artery from the pulmonary artery. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound, 2011
Effects of ultramorphological changes on adhesion to lased dentin—Scanning electron microscopy and transmission electron microscopy analysis
Authors: Moretto Simone G. , Azambuja Nilton , Arana‐Chavez Victor E. , Reis Andre F. , Giannini Marcelo , Eduardo Carlos de P. , De Freitas Patricia M. 
Published: October 2010

AbstractDentin irradiation with erbium lasers has been reported to alter the composite resin bond to this treated surface. There is still a lack of studies reporting the effect of erbium lasers on dentin organic content and elucidating how laser treatment could interfere in the quality of the resin–dentin interface. This study aimed to evaluate the effect of erbium laser irradiation on dentin morphology and microtensile bond strength (μTBS) of an adhesive to dentin. Seventy‐two dentin disks were divided into nine groups (n = 8): G1‐Control (600‐grit SiC paper); Er:YAG groups: G2‐ 250 mJ/4 Hz; G3‐ 200 mJ/4 Hz; G4‐ 180 mJ/10 Hz; G5‐ 160 mJ/10 Hz; Er,Cr:YSGG groups: G6‐ 2 W/20 Hz; G7‐ 2.5 W/20 Hz; G8‐ 3 W/20 Hz; G9‐ 4 W/20 Hz. Specimens were processed for cross‐sectional analysis by scanning electron microscopy (SEM) (n = 3), transmission electron microscopy (TEM) (n = 2), and adhesive interface (n = 3). Forty‐five dentin samples (n = 5) were restored and submitted to μTBS testing. ANOVA (α = 5%) revealed that G1 presented the highest μTBS values and irradiated groups did not differ from each other. TEM micrographs showed a superficial layer of denatured collagen fibrils. For SEM micrographs, it was possible to verify the laser effects extending to dentin subsurface presenting a rough aspect. Cross‐sectional dentin micrographs of this hybridized surface revealed a pattern of modified tags with ringlike structures around it. This in vitro study showed that erbium laser irradiation interacts with the dental hard tissue resulting in a specific morphological pattern of dentin and collagen fibrils that negatively affected the bond strength to composite resin. Microsc. Res. Tech., 2011. © 2010 Wiley‐Liss, Inc.
Secular and seasonal variations of winter monsoon weather patterns in Japan since the early 20th century
Authors: Hirano Junpei , Matsumoto Jun 
Published: October 2010

AbstractWinter weather conditions in Japan are characterized by a clear contrast between the regions on the Japan Sea side and those on the Pacific Ocean side of the Japanese Islands. Weather conditions in the Japan Sea side are characterized by snowfall or rainfall under winter monsoon outbreak conditions. However, the Pacific Ocean side has fine weather because the central ridges of mountains block snow clouds coming from the Japan Sea. This particular weather pattern can easily be recognized from the daily precipitation distributions. In this study, we used the daily precipitation data recorded by the Japan Meteorological Agency since 1901 to define winter monsoon weather pattern based on the contrast in the spatial distribution of daily precipitation between the regions on the Japan Sea side and those on the Pacific Ocean side. On the basis of seasonal changes in the occurrence frequencies of winter monsoon weather pattern, we delimited the beginning and ending pentads of the winter monsoon season for each year. We aimed to clarify the characteristics of long‐term variations in the lengths of the winter monsoon season and in the seasonal march of winter weather conditions in Japan since the early 20th century. The results revealed a significant decreasing trend in the lengths of the winter monsoon season since the early 20th century. We also found that the occurrence frequencies of winter monsoon weather pattern have been decreasing significantly since the early 20th century. In addition to the long‐term decreasing trend, we detected an abrupt decrease in the occurrence frequencies of winter monsoon weather pattern in the mid‐1980s, which suggests a weakening of the winter monsoon after the mid‐1980s. Copyright © 2010 Royal Meteorological Society
Differences in patellofemoral kinematics between weight‐bearing and non‐weight‐bearing conditions in patients with patellofemoral pain
Authors: Draper Christine E. , Besier Thor F. , Fredericson Michael , Santos Juan M. , Beaupre Gary S. , Delp Scott L. , Gold Garry E. 
Published: October 2010

AbstractPatellar maltracking is thought to be one source of patellofemoral pain. Measurements of patellar tracking are frequently obtained during non‐weight‐bearing knee extension; however, pain typically arises during highly loaded activities, such as squatting, stair climbing, and running. It is unclear whether patellofemoral joint kinematics during lightly loaded tasks replicate patellofemoral joint motion during weight‐bearing activities. The purpose of this study was to: evaluate differences between upright, weight‐bearing and supine, non‐weight‐bearing joint kinematics in patients with patellofemoral pain; and evaluate whether the kinematics in subjects with maltracking respond differently to weight‐bearing than those in nonmaltrackers. We used real‐time magnetic resonance imaging to visualize the patellofemoral joint during dynamic knee extension from 30° to 0° of knee flexion during two conditions: upright, weight‐bearing and supine, non‐weight‐bearing. We compared patellofemoral kinematics measured from the images. The patella translated more laterally during the supine task compared to the weight‐bearing task for knee flexion angles between 0° and 5° (p = 0.001). The kinematics of the maltrackers responded differently to joint loading than those of the non‐maltrackers. In subjects with excessive lateral patellar translation, the patella translated more laterally during upright, weight‐bearing knee extension for knee flexion angles between 25° and 30° (p = 0.001). However, in subjects with normal patellar translation, the patella translated more laterally during supine, non‐weight‐bearing knee extension near full extension (p = 0.001). These results suggest that patellofemoral kinematics measured during supine, unloaded tasks do not accurately represent the joint motion during weight‐bearing activities. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:312–317, 2011
A rat model of massive rotator cuff tears
Authors: Liu Xuhui , Manzano Givenchy , Kim Hubert T. , Feeley Brian T. 
Published: October 2010

AbstractRotator cuff tears (RCTs) are the most common tendon injury seen in orthopedic patients. Massive RCT does not heal spontaneously and results in poor clinical outcomes. Muscle atrophy and fatty infiltration in rotator cuff muscles are major complications of chronic massive RCT and are thought to be the key factors responsible for the failure of attempted massive RCT repair. However, the pathophysiology of rotator cuff muscle atrophy and fat infiltration remains largely unknown, and no small animal model has been shown to reproduce the histologic and molecular changes seen in massive RCT. In this article, we report a novel rat massive RCT model, in which significant and consistent muscle atrophy and fat infiltration were observed in the rotator cuff muscles after rotator cuff tendon transection and denervation. The supraspinatus and infraspinatus muscle lost 25.4% and 28.9% of their wet weight 2 weeks after complete tendon transection, respectively. Six weeks after surgery, the average wet weight of supraspinatus and infraspinatus muscles decreased 13.2% and 28.3%, respectively. Significant fat infiltration was only observed in infraspinatus 6 weeks after tendon transection. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:588–595, 2011
Resolution of multicomponent mixture of amino acids using environmentally benign eluents: A green chromatographic approach
Authors: Mohammad Ali , Haq Nazrul , Siddiq Asma 
Published: October 2010

AbstractA new green TLC has been used for identifying and monitoring the migration behavior of amino acids through silica and kieselguhr static flat bed in contact of n‐butyl alcohol, ethyl acetate or ethylene glycol and their mixtures. From the point of view of chromatographic performance, a mixture of n‐butyl alcohol–70% aqueous ethylene glycol–ethyl acetate ratio 5:3:2 by volume proves to be more efficient than the individual components for separation of amino acids from their binary, ternary and quaternary mixtures and the chromatographic parameters like ΔRF, separation factor (α) and resolution (RS) for the separation were calculated. Effect of the presence of foreign substances such as metal cations, anions, vitamins and pesticides as impurities in the sample on the separation was also examined. Effect of substitution of butanol by various alcohols has been examined to assess the impact of hydrophobicity of alcohols on the separation of amino acids. The limits of detection for tyrosine, tryptophan, alanine, isoleucine, methionine and serine were found to be 0.10 μg/spot, whereas for lysine it is 0.05 μg/spot. Application of the selected TLC system for the identification and separation of amino acids present in drugs/pharmaceuticals has been performed.
Characterization of synthetic copolymers by interaction polymer chromatography: Separation by microstructure
Authors: Brun Yefim , Foster Peggy 
Published: October 2010

AbstractGradient elution of synthetic polymers has been studied both theoretically and experimentally using normal and reversed‐phase HPLC systems. An accurate equation describing the gradient elution of polymer‐homologous series in the context of continuous random‐flight model of a flexible polymer chain interacting with attractive surface of the porous material has been derived and experimentally verified against a series of narrow polystyrene standards. Both the theory and the experiment predict the existence of molar mass‐independent gradient elution at critical point of adsorption (CPA). The extension of the theory to synthetic copolymers predicts the existence of the CPA for statistical copolymers and describes its dependence on chemical composition and microstructure (blockiness) of the polymer chains. One of the important theoretical conclusions is that blockiness always increases the retention, so that blockier polymer chains elute later than their more random counterparts with the same chemical composition. This prediction has been confirmed experimentally using block and statistical styrene‐methylmethacrylate copolymers. Block copolymers do not have CPA and always elute between critical points of the corresponding homopolymers. The retention depends on the polymer molar mass and increases with the length of the blocks from a stronger absorbing monomer. These findings provide theoretical and experimental bases for separation of statistical and block copolymers by chemical composition and microstructure of polymer chains.
A review of clinical diagnostic applications of liquid chromatography–tandem mass spectrometry
Authors: Shushan Bori 
Published: October 2010

AbstractLiquid chromatography coupled with tandem mass spectrometry (LC/MS/MS) technology is emerging as a complementary method to traditional methodology used for clinical applications. Enhanced specificity and high‐throughput capabilities are providing significant benefits to clinical diagnostic laboratories conducting routine analyses. This technology is expected to expand rapidly as scientists focus on more complicated challenges that can be solved efficiently by adding LC/MS/MS to their arsenal of techniques. © 2010 Wiley Periodicals, Inc. Mass Spec Rev 29:930–944, 2010
Mass spectrometry‐based clinical proteomics: Head‐and‐neck cancer biomarkers and drug‐targets discovery
Authors: Matta Ajay , Ralhan Ranju , DeSouza Leroi V. , Siu K.W. Michael 
Published: October 2010

AbstractMass spectrometry (MS)‐based proteomics is a rapidly developing technology for both qualitative and quantitative analyses of proteins, and investigations into protein posttranslational modifications, subcellular localization, and interactions. Recent advancements in MS have made tremendous impact on the throughput and comprehensiveness of cancer proteomics, paving the way to unraveling deregulated cellular pathway networks in human malignancies. In turn, this knowledge is rapidly being translated into the discovery of novel potential cancer markers (PCMs) and targets for molecular therapeutics. Head‐and‐neck cancer is one of the most morbid human malignancies with an overall poor prognosis and severely compromised quality of life. Early detection and novel therapeutic strategies are urgently needed for more effective disease management. The characterizations of protein profiles of head‐and‐neck cancers and non‐malignant tissues, with unprecedented sensitivity and precision, are providing technology platforms for identification of novel PCMs and drug targets. Importantly, low‐abundance proteins are being identified and characterized, not only from the tumor tissues, but also from bodily fluids (plasma, saliva, and urine) in a high‐throughput and unbiased manner. This review is a critical appraisal of recent advances in MS‐based proteomic technologies and platforms for facilitating the discovery of biomarkers and novel drug targets in head‐and‐neck cancer. A major challenge in the discovery and verification of these cancer biomarkers is the typically limited availability of well‐characterized and adequately stored clinical samples in tumor and sera banks, collected using recommended procedures, and with detailed information on clinical, pathological parameters, and follow‐up. Most biomarker discovery studies use limited number of clinical samples and verification of cancer markers in large number of samples is beyond the scope of a single laboratory. The validation of these potential markers in large sample cohorts in multicentric studies is needed for their translation from the bench to the bedside. © 2010 Wiley Periodicals, Inc. Mass Spec Rev 29:945–961, 2010
The pre‐ovulatory luteinizing hormone surge is followed by down‐regulation of CYP19A1, HSD3B1, and CYP17A1 and chromatin condensation of the corresponding promoters in bovine follicles
Authors: Nimz Marika , Spitschak Marion , Fürbass Rainer , Vanselow Jens 
Published: October 2010

AbstractThe pre‐ovulatory luteinizing hormone (LH) surge induces an extensive molecular, physiological, and morphological reorganization of the bovine follicle. This study was designed to elucidate if chromatin modulation is involved in the LH‐induced gene regulation. Granulosa and theca of well‐characterized large bovine follicles were isolated before and after the LH surge. CYP19A1, HSD3B1, and CYP17A1 transcripts, which encode key enzymes of steroid hormone biosynthesis, were quantified by real‐time PCR (qPCR) and the degree of chromatin condensation was determined by DNase I protection assays. After LH, granulosa‐specific CYP19A1 and theca‐specific CYP17A1 transcripts were almost completely down‐regulated. Also, the abundance of HSD3B1 transcripts was reduced. The promoter chromatin of HSD3B1 and particularly of CYP19A1 was significantly less accessible to DNAse I in both cell types after LH, whereas the chromatin accessibility of the CYP17A1 promoter changed only in the theca. Correlation analysis revealed partly, highly significant negative correlations between transcript abundance and protection from DNase I digestion of the corresponding chromatin. The data strongly suggest that LH induces cell type‐ and gene‐specific chromatin condensation in the pre‐ovulatory bovine follicle. This epigenetic mechanism might be involved in the pre‐ovulatory down‐regulation of genes. Mol. Reprod. Dev. 77:1040–1048, 2010. © 2010 Wiley‐Liss, Inc.