bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of Wnt‐5A in interleukin‐1β–induced matrix metalloproteinase expression in rabbit temporomandibular joint condylar chondrocytes
Authors: Ge Xianpeng , Ma Xuchen , Meng Juanhong , Zhang Chenguang , Ma Kangtao , Zhou Chunyan 
Published: August 2009

AbstractObjectiveTo determine the possible involvement and regulatory mechanisms of Wnt‐5A signaling in interleukin‐1β (IL‐1β)–induced increase in matrix metalloproteinase 1 (MMP‐1), MMP‐3, MMP‐9, and MMP‐13 expression in temporomandibular joint (TMJ) condylar chondrocytes.MethodsPrimary rabbit condylar chondrocytes were treated with IL‐1β, purified Wnt‐5A protein, or both and transfected with Wnt‐5A expression vector. Expression of Wnt‐5A, MMP‐1, MMP‐3, MMP‐9, MMP‐13, and type II collagen, as well as cell morphologic changes, were examined. To explore the mechanisms of action of Wnt‐5A, the accumulation and nuclear translocation of β‐catenin, the transcription activity of the β‐catenin–Tcf/Lef complex, phosphorylated JNK, phosphorylated ERK, and phosphorylated p38 were analyzed. SP600125, a JNK inhibitor, was used to investigate the role of the JNK pathway in Wnt‐5A induction of MMP‐1, MMP‐3, MMP‐9, and MMP‐13.ResultsTreatment of rabbit condylar chondrocytes with IL‐1β up‐regulated Wnt‐5A expression. Purified Wnt‐5A protein and transfection with Wnt‐5A expression vector promoted the expression of MMP‐1, MMP‐3, MMP‐9, and MMP‐13. Wnt‐5A did not cause accumulation and nuclear translocation of β‐catenin or activation of the β‐catenin‐Tcf/Lef transcription complex. Instead, Wnt‐5A activated JNK, and an inhibitor of JNK blocked the Wnt‐5A–induced up‐regulated expression of MMPs.ConclusionThese findings indicate that IL‐1β up‐regulates Wnt‐5A, and the activation of Wnt‐5A signaling induces the expression of MMP‐1, MMP‐3, MMP‐9, and MMP‐13 via the JNK signaling pathway in rabbit TMJ condylar chondrocytes. Blockage of JNK signaling impairs the Wnt‐5A–induced up‐regulation of MMPs. Thus, Wnt‐5A may be associated with cartilage destruction by promoting the expression of MMPs.
Dendritic cells from spondylarthritis‐prone HLA–B27–transgenic rats display altered cytoskeletal dynamics, class II major histocompatibility complex expression, and viability
Authors: Dhaenens Maarten , Fert Ingrid , Glatigny Simon , Haerinck Saskia , Poulain Cécile , Donnadieu Emmanuel , Hacquard‐Bouder Cécile , André Claudine , Elewaut Dirk , Deforce Dieter , Breban Maxime 
Published: August 2009

AbstractObjectiveSpondylarthritis (SpA) is characterized by spinal and peripheral joint inflammation, frequently combined with extraarticular manifestations. Despite the well‐established association of SpA with the class I major histocompatibility complex (MHC) allele HLA–B27, there are still different, parallel hypotheses on the relationship between HLA–B27 and disease mechanisms. The present study was undertaken to investigate several characteristics of mature dendritic cells (DCs), which are believed to be essential for triggering disease in a model of SpA in HLA–B27–transgenic rats.MethodsWe combined different whole‐proteome approaches (2‐dimensional polyacrylamide gel electrophoresis and iTRAQ) to define the most aberrant molecular processes occurring in spleen DCs. Videomicroscopy and flow cytometry were used to confirm both cytoskeletal and class II MHC expression deficiencies.ResultsOur proteome studies provided evidence of up‐regulation of proteins involved in class I MHC loading, and unfolded protein response, along with a striking down‐regulation of several cytoskeleton‐reorganizing proteins. The latter result was corroborated by findings of deficient motility, altered morphology, and decreased immunologic synapse formation. Furthermore, class II MHC surface expression was reduced in DCs from B27‐transgenic rats, and this could be linked to differences in class II MHC–induced apoptotic sensitivity. Finally, we found reduced viability of the CD103+CD4− DC subpopulation, which likely exerts tolerogenic function.ConclusionTaken together, our findings have different important implications regarding the physiology of B27‐transgenic rat DCs, which have a putative role in spontaneous disease in these rats. In particular, the reduced motility and viability of putatively tolerogenic CD4+ DCs could play an important role in initiating the inflammatory process, resulting in SpA.
Adiponectin is a mediator of the inverse association of adiposity with radiographic damage in rheumatoid arthritis
Authors: Giles Jon T. , Allison Matthew , Bingham Clifton O. , Scott William M. , Bathon Joan M. 
Published: August 2009

AbstractObjectiveRecent reports have suggested that increasing adiposity may protect against radiographic damage in rheumatoid arthritis (RA). We explored the role of serum adipokines (adiponectin, resistin, and leptin) in mediating this association.MethodsPatients with RA underwent total‐body dual x‐ray absorptiometry for measurement of total and regional body fat and lean mass, abdominal computed tomography for measurement of visceral fat area, and radiographs of the hands and feet scored according to the modified Sharp/van der Heijde (SHS) method. Serum levels of adipokines were measured and cross‐sectional associations with radiographic damage were explored, adjusting for pertinent confounders. The associations of measures of adiposity with radiographic damage were explored with the introduction of adipokines into multivariable modeling as potential mediators.ResultsAmong the 197 patients studied, adiponectin demonstrated a strong association with radiographic damage, with the log SHS score increasing by 0.40 units for each log unit increase in adiponectin (P= 0.001) after adjusting for pertinent predictors of radiographic damage. Adiponectin independently accounted for 6.1% of the explainable variability in SHS score, a proportion comparable with rheumatoid factor, and greater than HLA–DRB1 shared epitope alleles or C‐reactive protein levels. Resistin and leptin were not associated with radiographic damage in adjusted models. An inverse association between visceral fat area and radiographic damage was attenuated when adiponectin was modeled as a mediator. The association of adiponectin with radiographic damage was stronger in patients with longer disease duration.ConclusionAdiponectin may represent a mechanistic link between low adiposity and increased radiographic damage in RA. Adiponectin modulation may represent a novel strategy for attenuating articular damage.
Mathematical modeling of the circadian rhythm of key neuroendocrine–immune system players in rheumatoid arthritis: A systems biology approach
Authors: Meyer‐Hermann Michael , Figge Marc Thilo , Straub Rainer H. 
Published: August 2009

AbstractObjectiveHealthy subjects and patients with rheumatoid arthritis (RA) exhibit circadian rhythms of the neuroendocrine–immune system. Understanding circadian dynamics is complex due to the nonlinear behavior of the neuroendocrine–immune network. This study was undertaken to seek and test a mathematical model for studying this network.MethodsWe established a quantitative computational model to simulate nonlinear interactions between key factors in the neuroendocrine–immune system, such as plasma tumor necrosis factor (TNF), plasma cortisol (and adrenal cholesterol store), and plasma noradrenaline (NA) (and presynaptic NA store).ResultsThe model was nicely fitted with measured reference data on healthy subjects and RA patients. Although the individual circadian pacemakers of cortisol, NA, and TNF were installed without a phase shift, the relative phase shift between these factors evolved as a consequence of the modeled network interactions. Combined long‐term and short‐term TNF increase (the “RA model”) increased cortisol plasma levels for only a few days, and cholesterol stores started to become markedly depleted. This nicely demonstrated the phenomenon of inadequate cortisol secretion relative to plasma TNF levels, as a consequence of adrenal deficiency. Using the RA model, treatment with glucocorticoids between midnight and 2:00 AM was found to have the strongest inhibitory effect on TNF secretion, which supports recent studies on RA therapy. Long‐term reduction of TNF levels by simulation of anti‐TNF therapy normalized cholesterol stores under “RA” conditions.ConclusionThese first in silico studies of the neuroendocrine–immune system in rheumatology demonstrate that computational biology in medicine, making use of large collections of experimental data, supports understanding of the pathophysiology of complex nonlinear systems.
Psychophysical and functional imaging evidence supporting the presence of central sensitization in a cohort of osteoarthritis patients
Authors: Gwilym Stephen E. , Keltner John R. , Warnaby Catherine E. , Carr Andrew J. , Chizh Boris , Chessell Iain , Tracey Irene 
Published: August 2009

AbstractObjectiveThe groin pain experienced by patients with hip osteoarthritis (OA) is often accompanied by areas of referred pain and changes in skin sensitivity. We aimed to identify the supraspinal influences that underlie these clinical manifestations that we consider indicative of possible central sensitization.MethodsTwenty patients with hip OA awaiting joint replacement and displaying signs of referred pain were recruited into the study, together with age‐matched controls. All subjects completed pain psychology questionnaires and underwent quantitative sensory testing (QST) in their area of referred pain. Twelve of 20 patients and their age‐ and sex‐matched controls underwent functional magnetic resonance imaging (MRI) while the areas of referred pain were stimulated using cold stimuli (12°C) and punctate stimuli (256 mN). The remaining 8 of 20 patients underwent punctate stimulation only.ResultsPatients were found to have significantly lower threshold perception to punctate stimuli and were hyperalgesic to the noxious punctate stimulus in their areas of referred pain. Functional brain imaging illustrated significantly greater activation in the brainstem of OA patients in response to punctate stimulation of their referred pain areas compared with healthy controls, and the magnitude of this activation positively correlated with the extent of neuropathic‐like elements to the patient's pain, as indicated by the PainDETECT score.DiscussionUsing psychophysical (QST) and brain imaging methods (functional MRI), we have identified increased activity with the periaqueductal grey matter associated with stimulation of the skin in referred pain areas of patients with hip OA. This offers a central target for analgesia aimed at improving the treatment of this largely peripheral disease.
Maternal caffeine consumption and risk of neural tube defects
Authors: Schmidt Rebecca J. , Romitti Paul A. , Burns Trudy L. , Browne Marilyn L. , Druschel Charlotte M. , Olney Richard S. 
Published: August 2009

AbstractBACKGROUND:Animal studies demonstrate teratogenic effects of caffeine, whereas human studies are inconclusive.METHODS:Associations between maternal caffeine consumption and neural tube defects (NTDs) by type of NTD (anencephaly, spina bifida, or encephalocele) were examined using data from the National Birth Defects Prevention Study (NBDPS). Total average daily caffeine from coffee, tea, soda, and chocolate consumption during the year before pregnancy was estimated for 768 mothers of infants with NTDs and 4143 mothers of infants without birth defects who gave birth during 1997 through 2002. Periconceptional use of caffeine‐containing medications was evaluated separately. Adjusted odds ratios (OR) and 95% confidence intervals (CI) associated with consumption of total caffeine and each caffeine source were estimated from logistic regression models.RESULTS:Positive associations were observed between spina bifida and total caffeine consumption (OR 1.4; 95% CI: 1.1–1.9) and each caffeine source except caffeinated tea, which showed a negative association with spina bifida (OR 0.7; 95% CI: 0.6–0.9). Associations with modestly increased risk of NTDs and encephalocele were also observed. The association between caffeine consumption and anencephaly differed by maternal race/ethnicity. No dose effects were found.CONCLUSIONS:Additional studies should confirm whether women who consume caffeine are at increased risk for pregnancies complicated by NTDs. Birth Defects Research (Part A) 2009. © 2009 Wiley‐Liss, Inc.
Generation of a syngeneic mouse model to study the intraperitoneal dissemination of ovarian cancer with in vivo luciferase imaging
Authors: Toyoshima Masafumi , Tanaka Yoshinori , Matumoto Mitsuyo , Yamazaki Miyuki , Nagase Satoru , Sugamura Kazuo , Yaegashi Nobuo 
Published: August 2009

AbstractIn order to facilitate the discovery and investigation of anti‐cancer therapeutics under physiological conditions, we have engineered the ovarian cancer cell line, HM‐1/luc, in mice. This cell stably expresses firefly luciferase and produces light that can be detected using an in vivo imaging system (IVIS). Parental HM‐1 cells cause severe carcinomatous peritonitis to B6C3F1 mice, but not to C57BL6 mice. Established HM‐1/luc cells showed pathologically similar findings to HM‐1 cells. HM‐1/luc cells were injected into the peritoneal cavity of B6C3F1 mice and IVIS 2000 was conducted weekly after inoculation to monitor intra‐peritoneal tumor growth. The mice were divided into three groups: non‐CDDP‐treated (control) and CDDP‐treated (0.2 and 0.4 mg). A disease‐suppressive effect of the CDDP was reflected by the significantly prolonged survival of the CDDP‐treated mice (control 23 ± 1.9 days, CDDP 0.2 mg 29.6 ± 2.9 days; p < 0.05); the total photon and area of flux were decreased. The optical imaging of intraperitoneal tumors via in vivo bioluminescence is effective for noninvasive monitoring and semi‐quantitative analysis. Our syngeneic mouse model has the relevant clinical features of ovarian cancer, which makes it a useful model for developing new ovarian cancer therapies. Copyright © 2009 John Wiley & Sons, Ltd.
Intense visible multicolored luminescence from lanthanide ion‐pair codoped NaGdF4 nanocrystals
Authors: Li Feng , Li Nan , Wang Meng , Xu Shukun , Zhang Hongyan 
Published: August 2009

AbstractLanthanide ion‐pair (Eu3+/Tb3+, Dy3+/Tb3+, Sm3+/Tb3+ and Eu3+/Dy3+) codoped NaGdF4 nanocrystals using Ce3+ as the sensitizer were prepared via the polyol method. The nanocrystals with different codoped lanthanide ion‐pairs retain their individual optical properties and the combined spectra can be detected using single‐wavelength excitation at about 251 nm. The combined spectra intensity ratios can be adjusted through control of the doping ions molar ratios. Excited with a UV lamp at 254 nm, the as‐prepared nanocrystals in aqueous solution emit intense visible emissions of different colors. The nanocrystals were coated with SiO2, to make them biocompatible. Copyright © 2009 John Wiley & Sons, Ltd.
A JNK inhibitor SP600125 induces defective cytokinesis and enlargement in P19 embryonal carcinoma cells
Authors: Nakaya Kohei , Ooishi Ryo , Funaba Masayuki , Murakami Masaru 
Published: August 2009

AbstractWhile analyzing the role of c‐Jun NH2‐terminal kinase (JNK) in neurogenesis in P19 embryonal carcinoma cells, we noticed that treatment with SP600125, a JNK inhibitor, increased the cell size markedly. SP600125‐induced enlargement of P19 cells was time‐ and dose‐dependent. The increased cell size in response to SP600125 was also detected in B6mt‐1 embryonic stem cells. SP600125 treatment inhibited cell growth and increased DNA contents, indicating the inhibition of cell proliferation resulting from endoreduplication. Concurrently, the gene expression of p21, a regulator of G2/M arrest as well as G1 arrest, was increased in cells treated with SP600125. The increased cell size in response to SP600125 was detected even in P19 cells treated with colcemide, an inhibitor of cell cycle progression at the metaphase. The present study suggests that treatment with SP600125 progresses the cell cycle, skipping cytokinesis in P19 cells. Copyright © 2009 John Wiley & Sons, Ltd.
Changes of glycogen content in liver, skeletal muscle, and heart from fasted rats
Authors: Kokubun Eduardo , Hirabara Sandro M. , Fiamoncini Jarlei , Curi Rui , Haebisch Horst 
Published: August 2009

AbstractGlycogen content of white and red skeletal muscles, cardiac muscle, and liver was investigated in conditions where changes in plasma levels of non‐esterified fatty acids (NEFA) occur. The experiments were performed in fed and 12 and 48 h‐fasted rats. The animals were also submitted to swimming for 10 and 30 min. Glycogen content was also investigated in both pharmacologically induced low plasma NEFA levels fasted rats and pharmacologically induced high plasma NEFA levels fed rats. The participation of Akt and glycogen synthase kinase‐3 (GSK‐3) in the changes observed was investigated. Plasma levels of NEFA, glucose, and insulin were determined in all conditions. Fasting increased plasma NEFA levels and reduced glycogen content in the liver and skeletal muscles. However, an increase of glycogen content was observed in the heart under this condition. Akt and GSK‐3 phosphorylation was reduced during fasting in the liver and skeletal muscles but it remained unchanged in the heart. Our results suggest that in conditions of increased plasma NEFA levels, changes in insulin‐stimulated phosphorylation of Akt and GSK‐3 and glycogen content vary differently in liver, skeletal muscles, and heart. Akt and GSK‐3 phosphorylation and glycogen content are decreased in liver and skeletal muscles, but in the heart it remain unchanged (Akt and GSK‐3 phosphorylation) or increased (glycogen content) due to consistent increase of plasma NEFA levels. Copyright © 2009 John Wiley & Sons, Ltd.
A Descriptive Approach to Gibbs Fundamental Equation and Equilibrium Conditions in Reacting Multi‐component Systems based on the First and Second Law of Thermodynamics
Authors: Klein H. 
Published: August 2009

AbstractThe Gibbs fundamental equation for multi‐component systems can be derived directly from the first and second law of thermodynamics. This approach has been outlined already by Haase [1], but the approach adopted in that work required the assumption that, besides dissipation of mechanical work, chemical reactions contribute to a generation of entropy within the system. In this paper, a derivation is presented, which shows that the expression for the entropy generation arises automatically without any further assumptions. It is shown that the correct driving forces for chemical reactions can be obtained over the whole composition range only if the adequate caloric equation of state for the molar entropy is used for the components in the mixture. Two chemical reactions of industrial importance are presented to show this impact over the entire range of educt and product compositions. Furthermore, it is shown that the different approaches based on molar free enthalpy of reaction as the driving force of chemical reactions, minimum Gibbs free enthalpy and functional equations based on equilibrium constants, are inherently similar since all of them reflect more or less the second law of thermodynamics.
Effect of a Magnetic Field on a Micropolar Fluid Flow in the Vicinity of an Axisymmetric Stagnation Point on a Circular Cylinder
Authors: Abdel‐Rahman G. M. 
Published: August 2009

AbstractThe effect of a magnetic field on a micropolar fluid flow in the vicinity of an axisymmetric stagnation point on a circular cylinder is studied numerically. The governing conservation equations of continuity, momentum and angular momentum are partial differential equations which are transformed into a system of ordinary differential equations by using the usual similarity transformations. The resulting system of coupled non‐linear ordinary differential equations is solved numerically by using the shooting method. The numerical results indicate the velocity, angular velocity and pressure distributions for different parameters of the problem including Reynolds number, magnetic parameter and dimensionless material properties, etc. In addition, the effect of the pertinent parameters on the local skin friction coefficient and the couple stress are discussed numerically and illustrated graphically.
Chemodivergent Metathesis of Dienynes Catalyzed by Ruthenium–Indenylidene Complexes: An Experimental and Computational Study
Authors: Clavier Hervé , Correa Andrea , Escudero‐Adán Eduardo C. , Benet‐Buchholz Jordi , Cavallo Luigi , Nolan Steven P. 
Published: August 2009

AbstractA study on the enyne metathesis reaction leading to the formation cyclic compounds using ruthenium–indenylidene complexes is presented. Several 1,11‐dien‐6‐ynes have been subjected to ruthenium metathesis cyclization by using ruthenium–indenylidene complexes bearing various phosphine and N‐heterocyclic carbene (NHC) ligands. Interestingly, for some substrates chemodivergent metathesis occurs and is a function of the catalyst employed. This led us to investigate the competing “ene‐then‐yne” or “yne‐then‐ene” reaction pathways apparently at play in these systems using both experimental observations and DFT calculations. Experimental and computational studies were found in good agreement and permit to conclude that for phosphine‐containing catalysts, the “ene‐then‐yne” pathway is exclusively adopted. On the other hand, for catalysts bearing NHC ligands, both pathways are possible.
ChemInform Abstract: Electronic Structure of Cadmium Selenogallate CdGa2Se4 as Studied Using ab initio Calculations and X‐Ray Photoelectron Spectroscopy.
Authors: Lavrentyev A. A. , Gabrelian B. V. , Nikiforov I. Ya. , Parasyuk O. V. , Khyzhun O. Yu. 
Published: August 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Technetium(IV) Halides Predicted from First‐Principles.
Authors: Weck Philippe F. , Kim Eunja , Poineau Frederic , Rodriguez Efrain E. , Sattelberger Alfred P. , Czerwinski Kenneth R. 
Published: August 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: First Mixed Alkaline Uranyl Molybdates: Synthesis and Crytal Structures of CsNa3 [(UO2)4O4(Mo2O8)] and Cs2Na8 [(UO2)8O8(Mo5O20)].
Authors: Nazarchuk Evgeny V. , Siidra Oleg I. , Krivovichev Sergey V. , Malcherek Thomas , Depmeier Wulf 
Published: August 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Strontium Aluminum Antimony Oxide with Kagome Layers: Sr1.5+xSb5+2‐xAl5+xO14 (x ≈ 0.21).
Authors: Letrouit A. , Boudin S. , Retoux R. 
Published: August 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: High‐Temperature Synchrotron X‐Ray Diffraction Study of LaMn7O12.
Authors: Okamoto H. , Karppinen M. , Yamauchi H. , Fjellvaag H. 
Published: August 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.