bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ankyrins are essential for synaptic integrity of photoreceptors in the mouse outer retina
Authors: Perez Ross M , Campbell Jay , Goswami-Sewell Debalina , Venkatraman Rajashree , Gomez Cesiah C , Bagnetto Caitlin , Lee Audrey , Mattos Marlon F , Hoon Mrinalini , Zuniga-Sanchez Elizabeth 
Published: February 2025

ABSTRACTThe mammalian visual system consists of two distinct pathways: rod- and cone-driven vision. The rod pathway is responsible for dim light vision whereas the cone pathway mediates daylight vision and color perception. The distinct processing of visual information begins at the first synapse of rod and cone photoreceptors. The unique composition and organization of the rod and cone synapse is what allows information to be parsed into the different visual pathways. Although this is a critical process for vision, little is known about the key molecules responsible for establishing and maintaining the distinct synaptic architecture of the rod and cone synapse. In the present study, we uncovered a new role for Ankyrins in maintaining the synaptic integrity of the rod and cone synapse. Loss of Ankyrin-B and Ankyrin-G results in connectivity defects between photoreceptors and their synaptic partners. Ultrastructure analysis of the rod and cone synapse revealed impaired synaptic innervation, abnormal terminal morphology, and disruption of synaptic connections. Consistent with these findings, functional studies revealed impairedin vivoretinal responses in animals with loss of Ankyrin-B and Ankyrin-G. Taken together, our data supports a new role for Ankyrins in maintaining synaptic integrity and organization of photoreceptor synapses in the mouse outer retina.SIGNFICANCE STATEMENTThe first synapse in the outer retina begins to process visual information into two distinct pathways. This is largely attributed to the different composition and organization of the rod and cone synapse. Although the structural integrity of the rod and cone synapse is critical for normal vision, little is known about the key molecules responsible for maintaining the unique structure of the different photoreceptor synapses. In this study, we demonstrate a new function for the cytoskeletal scaffolding proteins, Ankryin-B and Ankyrin-G in the mouse outer retina. We found Ankyrin-B and Ankyrin-G are both required for proper retinal connectivity, where loss of these molecules leads to synaptic defects and impaired retinal responses.
The minor antennae of photosystem II contribute to qH-energy dissipation inArabidopsis
Authors: Bru Pierrick , Crepin Aurélie , Provot Yolande , Guardini Zeno , Bassi Roberto , Dall’Osto Luca , Malnoë Alizée 
Published: February 2025

AbstractPhotosynthesis is a biological process that converts light energy into chemical energy. Excessive light can damage the photosynthetic machinery, so plants have evolved photoprotective mechanisms such as non-photochemical quenching (NPQ). Among the NPQ mechanisms, qH is a form of sustained quenching, dependent on LIPOCALIN IN THE PLASTID (LCNP) and repressed by SUPPRESSOR OF QUENCHING 1 (SOQ1), protecting against abiotic stress. Recently, we showed inArabidopsis thalianathat qH can occur in the major light-harvesting complexes (Lhcb1, Lhcb2, Lhcb3) but independently of any specific major antenna. Interestingly, in mutants with little or no accumulation of major antennae (koLHCII, lhcb1, cpsrp43), qH can still be induced. Here, we show that the minor antennae can be quenched by qH and remain quenched once isolated. To investigate the role of minor antennae in qH, we combined thesoq1mutant, which displays high qH, with mutations in each minor antenna type (Lhcb4, Lhcb5, or Lhcb6), or with a mutant lacking all minor antennae. None are strictly required for qH to occur. Still, the absence of Lhcb6 decreases qH induction likely due to an indirect effect from the slower electron transport rate and/or a different macro-organization of photosynthetic complexes in the thylakoids. Overall, this work demonstrates that the minor antennae are a secondary target for qH and could serve as an additional safety valve for photoprotective energy dissipation during prolonged stress.
Stochastic model for the turbulent ocean heat flux under Arctic sea ice
Authors: Toppaladoddi S. , Wells A. J. 
Published: February 2025

The physics of planetary climate features a variety of complex systems that are challenging to model as they feature turbulent flows. A key example is the heat flux from the upper ocean to the underside of sea ice which provides a key contribution to the evolution of the Arctic sea ice cover. Here, we develop a model of the turbulent ice-ocean heat flux using coupled ordinary stochastic differential equations to model fluctuations in the vertical velocity and temperature in the Arctic mixed layer. All the parameters in the model are determined from observational data. A detailed comparison between the model results and measurements made during the Surface Heat Budget of the Arctic Ocean (SHEBA) project reveals that the model is able to capture the probability density functions (PDFs) of velocity, temperature, and heat flux fluctuations. Furthermore, we show that the temperature in the upper layer of the Arctic Ocean can be treated as a passive scalar during the whole year of SHEBA measurements. The stochastic model developed here provides a computationally inexpensive way to compute an observationally consistent PDF of this heat flux and has implications for its parametrization in regional and global climate models.

Published by the American Physical Society
2025
Sustainable development practices in healthcare: a systematic review
Authors: Del Bene Luca , Leoni Giulia , Vermiglio Carlo , Zarone Vincenzo 
Published: February 2025

PurposeHealthcare organizations are rethinking their practices in response to the framework provided by the sustainable development goals (SDGs). The purpose of this paper is to explore whether, how and why sustainable development practices have been implemented in the healthcare sector.Design/methodology/approachThe study is based on a systematic literature review, carried out using Scopus and Web of Science databases. The papers were analyzed according to the five activity-based theoretical framework categories: purpose, subject, community, object and practices.FindingsResults reveal a lack of research regarding the integration of sustainable development and SDGs into managerial systems or functions. Moreover, they identify key areas of interest within sustainable development and healthcare practices, offering guidance for future research and strategies to better align healthcare with the SDGs.Originality/valueTo the best of the authors’ knowledge, this is the first paper that aims to summarize the scientific literature on this topic through the lens of the activity-based theoretical framework, allowing for an analysis of the literature that investigates how sustainable development practices shape organizational life.
Interplay of provider and customer factors for servitization success: a transaction cost theory approach
Authors: Lexutt Eva 
Published: February 2025

PurposeThis study examine how provider- and customer-related factors interact to influence servitization success. It adopts the transaction cost theory along with a configurational approach and hypothesizes that different configurations of five key conditions—service offering, specific investments, perceived customer opportunism, willingness for integration and demand uncertainty—can lead to servitization success or failure.Design/methodology/approachThe study applies fuzzy-set Qualitative Comparative Analysis (fsQCA) to a sample of 143 German manufacturers, addressing the complex causalities involved in servitization success.FindingsThe analysis identifies six sufficient configurations for servitization success and five for servitization failure. The findings reveal that servitization can succeed through various types of service offerings. While opportunism does not hinder success, the decision to offer an extensive service portfolio is influenced by anticipated opportunism and complex customer needs. Specific investments function primarily as drivers for success, particularly when combined with a limited service offering and complex customer needs. However, these investments can increase transaction costs when linked to an extensive service portfolio. Though not essential, customer integration emerges as a relevant success factor, acting as a safeguard against opportunism.Practical implicationsServitization can be successful even with opportunism. Developing methods to assess customers’ readiness for integration can mitigate opportunistic behavior and foster successful servitization.Originality/valueThis study advances servitization research by addressing the often-overlooked interplay between provider- and customer-related factors. Applying the transaction cost theory and a cutting-edge fsQCA, it contributes to the theoretical and methodological plurality of the field.
Efficacy of granulocyte and monocyte adsorptive apheresis on skin and joint manifestations of palmoplantar pustulosis with pustulotic arthro‐osteitis: A multicentric, prospective, observational study
Authors: Abe Namiko , Higashi Yuko , Tateishi Chiharu , Kanekura Takuro , Tsuruta Daisuke , Kobayashi Tomoko , Okubo Yukari 
Published: February 2025

AbstractGranulocyte and monocyte adsorptive apheresis (GMA) selectively removes activated granulocytes and monocytes from the peripheral blood. In 2012, GMA was approved in Japan as a treatment for generalized pustular psoriasis and localized pustular psoriasis or palmoplantar pustulosis (PPP). Limited evidence from case reports and monocentric studies suggested that GMA is an effective treatment for skin and joint symptoms of PPP with pustulotic arthro‐osteitis (PAO). The present, prospective, observational study was performed at three dermatology departments in Japan to evaluate the efficacy and safety of GMA in patients with PPP with PAO. Between April 2017 and December 2020, two male and 12 female patients with PPP and PAO were enrolled. Their mean age, mean duration of skin manifestations, and mean duration of PAO symptoms was 52.8 years, 51.2 months, and 44.9 months, respectively. GMA was applied weekly over five sessions. The skin and joint symptoms were assessed at baseline, post‐GMA, and at the 3‐month follow‐up. A total of 12 patients completed five GMA sessions, and two patients discontinued the treatment because of adverse events. Thus, 12 patients were finally assessed post‐GMA, and 10 patients were assessed at the 3‐month follow‐up. The assessment of GMA efficacy demonstrated that the skin symptoms had remarkably improved and improved in 33.3% (4/12) and 70% (7/10) of the patients post‐GMA and at the 3‐month follow‐up, respectively. Furthermore, the joint symptoms had remarkably improved in 66.7% (8/12) and 60% (6/10) of the patients post‐GMA and at the 3‐month follow‐up, respectively. These results suggest that GMA is effective in treating the skin and joint symptoms of PPP with PAO.
What Alternative Do I Have?: Syrian Immigrant Entrepreneurs and Interest‐Based Financing in Norway
Authors: Tobin Sarah A. 
Published: February 2025

ABSTRACTSyrian refugee entrepreneurs in Norway navigate a financing and support system that they find largely exclusive and unaccepting when it comes to launching and operating a new business. Using a case study of a Syrian Muslim immigrant entrepreneur and a lecture on the topic by a religious leader in a major mosque in Oslo, this paper utilizes a theoretical framework on moral economies and entrepreneurship to argue that there is a “mismatch” between the interests and needs of Syrian entrepreneurs for financial and social support, with the extremely limited offerings available in Norway. This leaves the entrepreneurs challenged to navigate the financial and moral issues on their own. The paper explores four key policy implications along the way that would enhance the possibilities for Muslim immigrant entrepreneurs to access and utilize financial and social support for opening and running a business in Norway. The paper concludes by linking the financial exclusion experienced by the Syrian entrepreneurs to their broader experiences attempting to integrate and become a positive part of Norwegian society through entrepreneurship, which is challenged in this context of exclusion.
Aligning Sustainable Finance and Fintech to Promote an Integrated Approach to Refugee Finance
Authors: Aassouli Dalal , Hajian Atieh , Asutay Mehmet , Jureidini Rajai Ray 
Published: February 2025

ABSTRACTThe paper aims to assess the global landscape of refugee finance with an overview of global refugee‐related financing mechanisms and instruments. Despite the growing refugee population worldwide, most refugee financing mechanisms are characterized by their post hoc, grant‐based, small‐scale, short‐term, and unpredictable nature. This study will analyze the quantum of refugee finance supplied by different donors and intermediary types, the instruments through which they deploy refugee finance, and to where this financing is directed. It will then evaluate their effectiveness in addressing refugee financial inclusion and empowerment. The paper finally suggests alternative instruments and financing mechanisms leveraging Islamic finance, sustainable finance, and fintech. Two key mechanisms are proposed: a Refugee Empowerment Sukuk and a blended finance structure adopting a results‐based financing approach. The study recommends developing a proactive approach using event‐based financing with early warning triggers, leveraging fintech solutions for traceability and efficiency, and scaling up result‐based financing in social impact and humanitarian financing.
Reduced Alcohol Consumption Following Ablation of Cholinergic Interneurons in the Nucleus Accumbens of Wistar Rats
Authors: Loftén Anna , Cadeddu Davide , Danielsson Klara , Stomberg Rosita , Adermark Louise , Söderpalm Bo , Ericson Mia 
Published: February 2025

ABSTRACTAlcohol use disorder is a severe mental health condition causing medical consequences and preterm death. Alcohol activates the mesolimbic dopamine system leading to an increase of extracellular dopamine (DA) in the nucleus accumbens, an event that is associated with the reinforcing effects of alcohol. Cholinergic interneurons (CIN) are important modulators of accumbal DA signalling, and depletion of accumbal CIN attenuates the alcohol‐induced increase in extracellular DA. The aim of this study was to explore the functional role of accumbal CIN in alcohol‐related behaviour. To this end, ablation of CIN was induced by local administration of anticholine acetyltransferase‐saporin bilaterally into the nucleus accumbens of male Wistar rats. Alcohol consumption in ablated and sham‐treated rats was studied using a two‐bottle‐choice intermittent alcohol consumption paradigm. Rats with depleted CIN consumed significantly less alcohol than sham‐treated controls. No differences in sucrose preference, motor activity, water intake or weight gain were noted between treatment groups, suggesting that the ablation selectively affected alcohol‐related behaviour. In conclusion, this study further supports a role for accumbal CIN in regulating alcohol‐consummatory behaviour.
First application of three‐dimensional light sheet fluorescence microscopy to human testicular tumors: New perspectives in histopathology
Authors: Pinkert‐Leetsch Diana , Kareem Ahmad , Merz Simon F. , Teckentrup Marc , Fichtner Alexander , Bremmer Felix , Missbach‐Guentner Jeannine 
Published: February 2025

AbstractBackgroundTesticular tumors are among the most frequently diagnosed cancers in young men. The consequences of this diagnosis are orchiectomies, severely restricting fertility. For these young patients, a comprehensive diagnostics would be desirable, achieving a refined diagnosis and improved therapeutic patient stratification.ObjectiveThe aim of this study was to use three‐dimensional (3D) light sheet fluorescence microscopy (LSFM) to analyze a complete testicular tumor punch at subcellular resolution—allowing a detailed diagnostic assessment of the entire punch.Materials and methodsTissue punches (3 and 5 mm diameter) were taken from paraffin blocks of four miscellaneous testicular tumors. After deparaffinization and clearing using benzoic acid/benzyl benzoate, a label‐free LSFM autofluorescence imaging was performed. In addition, TO‐PRO‐3 nuclear stain was applied to several punches. After the scan, the samples were embedded in paraffin again and physically sectioned for conventional planar histology.ResultsBased on the specific autofluorescence, not only the general morphology of the tumor tissue was identified in LSFM datasets, but also diagnostic features like infiltrations, papillary and pagetoid tumor cell formations, germ cell neoplasia in situ and azoospermia. Subcellular characteristics such as vacuolated cytoplasm and pleomorphic nuclei could be detected at maximum magnification. After nuclear staining, virtual H&E sections were reconstructed from the LSFM data and tomographically visualized across the entire punch. Subsequent histology and immunohistochemistry after LSFM analyses is possible.DiscussionLSFM analysis of testicular tumors enables the detailed 2D/3D analysis of an entire tumor punch for assessment of relevant tumor characteristics due to its intrinsic fluorescence or with specific nuclear staining.ConclusionLSFM provides the technical basis for the analyses of complete testicular tumor biopsies, thus maximizing the spatial morphological and anatomical information. The subcellular 3D imaging of the tumor has the potential to identify new cancer imaging biomarkers that have additional diagnostic and prognostic value for patients.
Evaluation of a virtual multidisciplinary tracheostomy meeting for ward‐based patients
Authors: Tan Hannah B. , Yii Matthew , Prasad Jessica 
Published: February 2025

AbstractBackgroundTracheostomy is frequently performed in an intensive care unit setting to progress the care of intubated patients. A multidisciplinary team follows up tracheostomised patients once transferred to the ward. A virtual tracheostomy multidisciplinary meeting (MDM) was established with the aim to standardize care for these patients.MethodsA retrospective review of ward‐based patients discussed in a Tracheostomy MDM was performed at The Alfred Hospital, a tertiary hospital providing a state‐wide trauma service. Two groups were compared; (i) Outreach group comprising patients who were managed prior to the establishment of the Tracheostomy MDM on the 26 July 2021; (ii) Virtual Tracheostomy MDM (V‐MDM) group who were managed after the establishment of the Virtual Tracheostomy MDM. Primary outcome was decannulation time from ICU discharge.ResultsThere were 65 patients in the Outreach group over 134 weeks. There were 44 patients in the V‐MDM group over 94 weeks. There was a significant difference between groups in the median time to decannulation from ICU discharge (P = 0.007) and the overall time to decannulation (P = 0.029) with the V‐MDM group requiring a longer period with tracheostomy prior to decannulation. There were no significant differences in the overall hospital length of stay (LOS) or rate of adverse events.ConclusionImplementing a supplemental Tracheostomy MDM in a virtual format may not improve time to decannulation and can possibly delay decannulation compared to an established tracheostomy team led by ICU clinicians. Virtual Tracheostomy MDM may have a role in managing patients were a non‐virtual ICU‐led team is not feasible.
FGFR2/3 Gene Alterations and Clinical Outcomes in Advanced/Metastatic Urothelial Cancer in Japan: MONSTAR‐SCREEN Database Study
Authors: Matsubara Nobuaki , Osawa Takahiro , Abe Takashige , Oya Mototsugu , Nishimoto Koshiro , Iwahori Toshiyuki , Tsuchiya Hiroaki , Murota Maiko , Yoshida Masaki , Tatematsu Yohei , Nakano Yosuke , Eto Masatoshi , Nonomura Norio 
Published: February 2025

ABSTRACTAdvanced/metastatic urothelial cancer (a/m UC) still has a poor prognosis despite the recent medical advances. Recent studies demonstrated that fibroblast growth factor receptor (FGFR) gene alterations (GAs) may be driver genes for UC; however, the proportion of UC genetic panel testing in Japan remains low. We clarified the proportion of patients with FGFR2/3 GAs, treatment patterns, and clinical outcomes in a/m UC patients in Japan. This study was a descriptive epidemiological study using the MONSTAR‐SCREEN database, and 138 patients with a/m UC were evaluated. The primary endpoint was the proportion of patients with FGFR2/3 GAs. The secondary endpoints included treatment patterns, clinical outcomes, genomic status before and after treatment, etc. The proportion of FGFR GA‐positive patients in a/m UC was 11.9%. The most common FGFR mutation variant and fusion gene were S249C (4.4%) and FGFR3‐TACC3 fusion (3.7%), respectively. Fifty‐one patients were tested two or more times; a few changes were observed in the FGFR GA status, regardless of the treatment regimen. Co‐occurrence association was observed in FGFR1 with TET2, and in FGFR3 with CHEK2 or MLL2. During the first‐, second‐, and third‐line treatment, median progression‐free survival (PFS) of GA‐positive patients was 7.3, 2.9, and 6.2 months, while for GA‐negative patients, 6.9, 3.1, and 6.9 months, respectively. This study revealed that one in eight a/m UC patients had FGFR2/3 GAs, and a few changes were observed in FGFR GA status before and after treatment. Genetic testing will be beneficial for the selection of appropriate treatments after a diagnosis of a/m UC.
Affiliate Stigma and Its Predictors in Parents of Children With Autism in the Early Post‐diagnosis Phase
Authors: Wei Yao , Ji Yan , Chen Miaoying , Deng Tao , Liu Shaofei , Dai Yushen , Zhang Lifeng 
Published: February 2025

ABSTRACTBackgroundAffiliated stigma refers to internalized stigma among family members of stigmatized individuals. Affiliate stigma experienced by parents of children with autism plays a vital role in both the child's rehabilitation and the parents' well‐being, whereas the affiliate stigma and its possible predictors need to be further examined.AimsThe study aims to describe the characteristics of affiliate stigma experienced by parents of children with autism in the early post‐diagnosis period and to explore its predictors.MethodsThe study was conducted from September 2020 to March 2022 in Guangzhou, China. Participants included 116 parents of children newly diagnosed with autism who completed baseline self‐report measures of affiliate stigma (Affiliate Stigma Scale [ASS]), coping style (Simplified Coping Style Questionnaire [SCSQ]) and social support (Social Support Rating Scale [SSRS]) at the initial diagnosis (T1) and 1‐year follow‐up (T2). Autism features were assessed by parents using the Autism Behavior Checklist at T1. Generalized estimating equations were used to identify the key predictors of affiliate stigma.ResultsScores for parental affiliate stigma were 43.4 ± 13.7 at the initial diagnosis and 44.7 ± 15.9 one year later. Parents of children with siblings or co‐occurring conditions experienced higher levels of affiliate stigma, and negative coping styles and lower levels of subjective support predicted higher affiliate stigma during the first year after autism diagnosis.ConclusionsParents experienced moderate and stable affiliate stigma during the first year after their children's autism diagnosis, especially those with children who have siblings or co‐occurring conditions and thus need additional support. Interventions should be focused on reducing negative coping and providing more subjective social support.