bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of Albumin Using CdS/SiO2 Core/shell Nanoparticles as Fluorescence Probes
Authors: Zhu Changqing , Liu Meigui , Wang Peng , Cao Ming , Cao Chun 
Published: September 2009

AbstractCdS quantum dots (QD) were capped with SiO2 via a microemulsion method for reducing the toxicity and imparting the biocompatibility of the CdS QD. The resulting CdS/SiO2 core/shell nanoparticles (NP) showed an improved water‐solubility and stability even in pH 4.0 acidic medium. Their fluorescence could be effectively enhanced in the presence of bovine serum albumin (BSA), due to the passivation effect of BSA on the surface of the NP. Furthermore, the concentration dependence of the fluorescence intensity obeys the Langmuir‐type binding isotherm. Thus a novel fluorescence enhancement method for the determination of BSA has been developed using the less‐toxic CdS/SiO2 core/shell NP as probes. Under optimal conditions, the linear range of calibration curve is 0.6–30 µg·mL−1, and the detection limit is 0.18 µg·mL−1. Compared with the water‐soluble CdS NP without SiO2 shell, the CdS/SiO2 core/shell NP exhibited slightly lower fluorescence response to BSA as well as other coexisting substances, such as heavy and transition metals, due to the inhibition of SiO2 shell. The proposed method was applied to the quantification of BSA in synthetic and serum samples with satisfactory results.
Cyclin D1 and D3 overexpression predicts malignant behavior in thyroid fine‐needle aspirates suspicious for Hurthle cell neoplasms
Authors: Troncone Giancarlo , Volante Marco , Iaccarino Antonino , Zeppa Pio , Cozzolino Immacolata , Malapelle Umberto , Palmieri Emiliano A. , Conzo Giovanni , Papotti Mauro , Palombini Lucio 
Published: September 2009

AbstractBACKGROUND:Thyroid fine‐needle aspiration (FNA) samples that feature a follicular‐patterned, monotonous Hurthle (oncocytic) cell population cannot be diagnosed reliably. The authors of this report recently identified cyclin D3 overexpression on histologic sections of Hurthle cell carcinoma. In this study, they assessed the diagnostic value of cyclin D3 immunohistochemistry added to routine cytology.METHODS:Fifty‐one FNA samples that were suspicious for Hurtle cell neoplasia and that had histologic follow‐up (19 malignant cases) were examined. Cyclin D3 expression levels were evaluated in cell block preparations and were compared with levels of the closely related cyclin D1 protein.RESULTS:Greater than 25% positive cells were used as the cutoff point, as suggested by previous studies. Cyclin D1 and cyclin D3 were highly specific (100% for both) and fairly accurate (75% and 92%, respectively) in distinguishing between benign and malignant oncocytic lesions; the positive predictive value (PPV) for each was 100%. However, both cyclins D1 and D3 had low sensitivity (32% and 79%, respectively) and low negative predictive value (NPV) (71% and 89%, respectively). In contrast, by adopting balanced receiver operating characteristic‐derived positive cutoff values, cyclin D1 (≥6.5%) and cyclin D3 (≥7.5%) were found to be highly sensitive (100% for both) and accurate (90% and 94%, respectively); and the NPV was 100% for both. In contrast, cyclins D1 and D3 had low specificity (84% and 91%, respectively) and a low PPV (79% and 86%, respectively); however, these values improved in samples that were positive for both cyclins (sensitivity, 100%; specificity, 94%; PPV, 90%; NPV, 100%; and accuracy, 96%).CONCLUSIONS:Cyclin D3 increased the suspicion of malignancy in indeterminate oncocytic lesions; its diagnostic performance depended on the cutoff point used and was enhanced further when combined with cyclin D1. Cancer (Cancer Cytopathol) 2009. © 2009 American Cancer Society.
Cytopathology of alveolar soft part sarcoma
Authors: Wakely Paul E. , McDermott James E. , Ali Syed Z. 
Published: September 2009

AbstractBACKGROUND:Alveolar soft part sarcoma (ASPS) rarely is subjected to cytopathologic evaluation. With the exception of some very small series, the literature is limited to case reports. The objectives of the current study were to evaluate the cytomorphology of 10 ASPS cases on fine‐needle aspiration (FNA) or imprint cytology, review the literature, and highlight potential diagnostic pitfalls.METHODS:The authors searched their files for all lesions that were signed out as ASPS or suspicious for ASPS and searched the surgical pathology files for any cases of ASPS that had corresponding cytology. FNA was performed using the standard technique. Scrape preparations were performed on tissues that were sent fresh for frozen section examination.RESULTS:Ten cases of ASPS were retrieved from 7 patients (male‐to‐female ratio, 4:3; mean age, 22 years). All had subsequent tissue confirmation. Six specimens were from primary lesions, 3 specimens were from metastases, and 1 intraoperative smear was from a previously aspirated primary mass in a newly diagnosed patient. Anatomic sites of involvement included extremities (5 specimens), trunk/pelvis (3 specimens), oral cavity (1 specimen), and lung (1 specimen). Eight specimens were aspirates, and 2 specimens were intraoperative smears. Nine cases were diagnosed correctly as ASPS/consistent with ASPS, and 1 specimen was diagnosed as “tumor, not otherwise specified”. Cytomorphology included variably cellular smears composed of large cells with an enormous amount of finely granular or vacuolated cytoplasm, markedly enlarged nuclei/nucleoli, and bare nuclei. The cytomorphology of some cases revealed a strong similarity to renal cell carcinoma, clear cell type.CONCLUSIONS:The results of the current study indicated that ASPS has cytomorphology that overlaps with several other neoplasms, including renal cell carcinoma. Nonetheless, the morphologic features, when combined with the clinical presentation, radiologic findings, and ancillary testing, may allow for a specific diagnosis. Cancer (Cancer Cytopathol) 2009. © 2009 American Cancer Society.
Ex Situ Prepared Antimony Film Electrode for Electrochemical Stripping Measurement of Heavy Metal Ions
Authors: Jovanovski Vasko , Hočevar Samo B. , Ogorevc Bozidar 
Published: September 2009

AbstractThe antimony film electrode (SbFE) was prepared ex situ for anodic and adsorptive stripping voltammetric measurement of selected heavy metal ions. The electrode revealed good linearity for Cd(II) and Pb(II) in a nondeaerated solution of 0.01 M HCl in the examined concentration range from 25 to 80 μg L−1 with limits of detection of 1.1 μg L−1 for Cd(II) and 0.3 μg L−1 for Pb(II) and an excellent reproducibility. The preplated SbFE was also preliminary tested for measuring low levels of Ni(II) using adsorptive stripping voltammetry exhibiting good linearity and sensitivity in combination with only a 30 s deposition step.
Factors and interactions controlling infiltration, runoff, and soil loss at the microscale in a patchy Mediterranean semiarid landscape
Authors: Mayor Ángeles G. , Bautista Susana , Bellot Juan 
Published: September 2009

AbstractIn semiarid ecosystems, the transfer of water, sediments, and nutrients from bare to vegetated areas is known to be crucial to ecosystem functioning. Rainfall simulation experiments were performed on bare‐soil and vegetated surfaces, on both wet and dry soils, in semiarid shrub‐steppe landscapes of SE Spain to investigate the spatial and temporal factors and interactions that control the fine‐scale variation in water infiltration, runoff and soil loss, and hence the water and sediment flows in these areas. Three types of shrub‐steppe landscapes varying in plant community and physiography, and four types of plant patches (oak shrub, subshrub, tussock grass, and short grass mixed with chamaephytes) were studied. Higher infiltration and lower runoff and soil loss were measured on vegetation patches than on bare soils, for both dry and wet conditions. The oak‐shrub patches produced no runoff, while the subshrub patches showed the highest runoff and soil loss. Despite these differences among patch types, the influence of vegetation patch type on the variables analysed was not significant. The response of bare soil surfaces clearly varied between landscape types, yet the differences were only relevant under dry soil conditions. Stone cover, particularly the cover of embedded stones, and crust cover, were the key explanatory variables for the hydrological behaviour of bare soils. The study documents quantitatively how bare soils and vegetation patches function as runoff sources and runoff sinks, respectively, for a wide range of soil moisture conditions, and illustrates that landscape‐type effects on bare‐soil runoff sources may also exert an important control on the site hydrology, while the role of the vegetation patch type is less important. The effects of the control factors are modulated by antecedent soil moisture, with dry soils showing the most contrasting soil water infiltration between landscapes and surface types. Copyright © 2009 John Wiley & Sons, Ltd.
Correlation between losses of IGH or its segments and deletions of 13q14 in t(11;14) (q13;q32) multiple myeloma
Authors: Trakhtenbrot Luba , Hardan Izhar , Koren‐Michowitz Maya , Oren Shirley , Yshoev Galina , Rechavi Gideon , Nagler Arnon , Amariglio Ninette 
Published: September 2009

AbstractMultiple myeloma (MM) is a malignancy of the plasma cells (PCs) characterized by a wide variety of genetic and chromosomal abnormalities. In recent years, major attention was drawn to the significance of chromosomal aberrations involving chromosome arm 13q and the IGH region on chromosome band 14q32 as a prognostic indicator in MM. In this study we applied a combined cell morphology and FISH method for the analysis of coexistence of t(11;14)(q13;q32) with deletions of the long arm of chromosome 13 (Δ13) in PCs from 51 MM patients using several probes for the 13q14, 11q13, and IGH regions. We found 15 different variants of the t(11;14) that are the consequence of different 11q13 breakpoints and various deletions of Variable (del IGH Var) or Constant (del IGH Const) IGH segments and also duplications and losses of the IGH gene on the normal nontranslocated chromosome 14 as well as IGH/Cyclin D1 (CCND1) fusion on der(14) and CCND1/IGH fusions on der(11). A strong association between Δ13 and specific variants of t(11;14) was found: variants with deletion of the IGH gene or its segments were found only in MM cases with deleted chromosome 13, while the common translocation t(11;14) was found only in the MM cases with normal chromosome arm 13q. In contrast, we did not find any association between Δ13 and deletions of the IGH gene or its segments in the MM patients with t(4;14)(p16;q32). © 2009 Wiley‐Liss, Inc.
Mathematics model for heat and mass transfer on an adsorption bed
Authors: Yang Peizhi 
Published: September 2009

AbstractIn a solid adsorption refrigeration system, the real cycle with non‐equilibrium adsorption is different from the ideal cycle with equilibrium adsorption. To investigate the heat and mass transfer process inside the adsorption bed, a model was established. Mathematical models were then solved by numerical method. The best cycle time of 24 minutes was ascertained through the strategy of SCP priority and paying attention to COP. The influence of the length of adsorption cell tube to the performance of refrigeration system is discussed. © 2009 Wiley Periodicals, Inc. Heat Trans Asian Res; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/htj.20271 Copyright © 2009 Wiley Periodicals, Inc.
Using GIS and a digital elevation model to assess the effectiveness of variable grade flow diversion terraces in reducing soil erosion in northwestern New Brunswick, Canada
Authors: Yang Qi , Zhao Zhengyong , Chow Thien Lien , Rees Herb W. , Bourque Charles P.‐A. , Meng Fan‐Rui 
Published: September 2009

AbstractFlow diversion terraces (FDT) are commonly used beneficial management practice (BMP) for soil conservation on sloped terrain susceptible to water erosion. A simple GIS‐based soil erosion model was designed to assess the effectiveness of the FDT system under different climatic, topographic, and soil conditions at a sub‐basin level. The model was used to estimate the soil conservation support practice factor (P‐factor), which inherently considered two major outcomes with its implementation, namely (1) reduced slope length, and (2) sediment deposition in terraced channels. A benchmark site, the agriculture‐dominated watershed in northwestern New Brunswick (NB), was selected to test the performance of the model and estimated P‐factors. The estimated P‐factors ranged from 0·38–1·0 for soil conservation planning objectives and ranged from 0·001 to 0·45 in sediment yield calculations for water‐quality assessment. The model estimated that the average annual sediment yield was 773 kg ha−1 yr −1 compared with a measured value of 641 kg ha−1 yr−1. The P‐factors estimated in this study were comparable with predicted values obtained with the revised universal soil loss equation (RUSLE2). The P‐factors from this study have the potential to be directly used as input in hydrological models, such as the soil and water assessment tool (SWAT), or in soil conservation planning where only conventional digital elevation models (DEMs) are available. Copyright © 2009 John Wiley & Sons, Ltd.
Using GIS for modelling the impact of current climate trend on the recharge area of the S. Susanna spring (central Apennines, Italy)
Authors: Spadoni M. , Brilli M. , Giustini F. , Petitta M. 
Published: September 2009

AbstractThough the S. Susanna spring system is one of the biggest water sources in the central Apennines, its hydrogeological dynamics have been scarcely investigated. This study tried to clarify some of the factors controlling the recharge/discharge processes of this spring by modelling the available climate series, water balance equations and new isotopic and quantitative data, using statistical and raster overlay functions embedded in a Geographic Information System (GIS). Oxygen and hydrogen isotopic data were recorded monthly over a 2‐year period at the spring itself and in eight rain gauges in Reatini Mountains. The effective infiltration rate was calculated using the Kennessey coefficients and the Turc equation. Finally, the recharge area was identified with the help of an expert evaluation procedure. Local δ18O and δD versus altitude regression curves were used to validate the digital recharge model by comparing their expected values with the values actually measured.Recharge process was framed within the perspective of the ongoing local climate trends. The current discharge rate of 4·1 m3·s−1 is significantly lower than the average value of 5·5 m3·s−1 measured up to the 1980s, confirming the fall in the recharge/discharge rate. The hydrogeological system shows a delayed response, due to an average groundwater residence time in the aquifer, which is estimated to be about 15/20 years on the basis of the offset between calculated and observed isotope data at the main spring. For this reason the system is presently not equilibrated and is gradually changing towards a final equilibrium discharge estimated in about 3·4 m3·s−1. Copyright © 2009 John Wiley & Sons, Ltd.
A high‐altitude temporary spring in a compartmentalized carbonate aquifer: the role of low‐permeability faults and karst conduits
Authors: Petrella Emma , Capuano Paolo , Carcione Massimiliano , Celico Fulvio 
Published: September 2009

AbstractThe aim of this research was to refine the actual conceptual model related to the activation of high‐altitude temporary springs within the carbonate Apennines in southern Italy. The research was carried out through geophysical, hydrogeological, hydrochemical and isotopic investigations at the Acqua dei Faggi experimental site during five hydrologic years.The research demonstrated that, in carbonate aquifers where low‐permeability faults cause the aquifer system to be compartmentalized, high‐altitude temporary springs may be recharged by groundwater. In such settings, neither surface water infiltration in karst systems nor perched temporary aquifers play a role of utmost importance. The rare (once or a few time a year) activation of such springs is due to the fact that groundwater unusually reach the threshold head that allows the spring to flow. The activation of the studied high‐altitude temporary spring also depended on relationships between a low‐permeability fault core and a karst system that locally interrupts the low‐permeability barrier. In fact, when the hydraulic head did not reach the karst system, the concentrated head loss within the fault core did not allow the spring to flow, because the groundwater entirely flowed through the fault towards the downgradient compartment. Copyright © 2009 John Wiley & Sons, Ltd.
Dynamics of deformation and water flow in heterogeneous porous media and its impact on soil properties
Authors: Wang Shih‐Jung , Hsu Kuo‐Chin 
Published: September 2009

AbstractThe interaction of geomechanics and flow within a soil body induces deformation and pore pressure change. Deformation may change hydrogeological and elastic properties, which alters the mechanical behaviour and results in non‐linearity. To investigate this interaction effect in a heterogeneous porous medium, a stochastic poroelastic model is proposed. Monte Carlo simulations are performed to determine the mean and uncertainty of the parameter changes, displacement, and change in pore water pressure. Hydraulic conductivity is treated as the only random variable in the coupled geomechanics‐flow system due to its large variation compared to other mechanical and hydrogeological properties in natural environments. The three considered non‐linear models for the interaction between parameters and deformation are those that consider (1) porosity and hydraulic conductivity; (2) porosity and Young's modulus; and (3) a combined effect that includes porosity, hydraulic conductivity, and Young's modulus. Boundary effects on the coupled system are also explored. The relationships between changes of porosity, hydraulic conductivity, and Young's modulus are analytically shown to be non‐linear. Among the considered parameters, the deformation effect induces the largest reduction in hydraulic conductivity. The deformation‐induced change in hydraulic conductivity shows the most significant effect on the mean and variance of the change in pore water pressure and displacement, while changes in Young's modulus have the least effect. When the deformation effect is considered, the superposition relationship does not exist in the mean displacement and mean change in pore water pressure for the three scenarios considered; it exists for the case without deformation effects. Deformation also causes a reduction in the effective hydraulic conductivity for the whole domain. The scenario that considers both loading and discharge boundaries has larger changes in hydrogeological and geo‐mechanical parameters than those in scenarios that consider loading and discharge boundaries separately. The results indicate that the interaction between deformation and changes in parameters has a profound effect on the poroelastic system. The effect of deformation should thus be considered in modelling and practice. Copyright © 2009 John Wiley & Sons, Ltd.
Hydrological modelling of Ethiopian catchments using limited data
Authors: Mekonnen Muluneh A. , Wörman Anders , Dargahi Bijan , Gebeyehu Admasu 
Published: September 2009

AbstractThe hydrological component of the soil and water assessment tool (SWAT) model is adapted for two Ethiopian catchments based on primary knowledge of the coherence spectrum between rainfall and stream flow data. Spectrum analysis using the available nearby climatic data is made to limit the temporal and spatial scales (inverse rate coefficients) subject to the calibration of compartmentalized runoff models. The exclusion of unwarranted time scales in the calibration implies that the model efficiency (r2 values) decrease only moderately between calibration and validation, and the optimization is focused on warranted problems. On the basis of the available data for the two Ethiopian catchments, the implication is that only periods longer than about 50 days can be reliably evaluated in the model. The model structure of SWAT for the surface runoff and groundwater flow response is modified to make the time scales consistent with the results of the spectrum analysis. An optimization algorithm is developed to constrain and combine the model parameters with the spectrum analysis results. Copyright © 2009 John Wiley & Sons, Ltd.
Spatial variations in throughfall in a Moso bamboo forest: sampling design for the estimates of stand‐scale throughfall
Authors: Shinohara Yoshinori , Onozawa Yuka , Chiwa Masaaki , Kume Tomonori , Komatsu Hikaru , Otsuki Kyoichi 
Published: September 2009

AbstractWe investigated the spatial and seasonal variations in throughfall (Tf) in relation to spatial and seasonal variations in canopy structure and gross rainfall (Rf) and assessed the impacts of the variations in Tf on stand‐scale Tf estimates. We observed the canopy structure expressed as the leaf area index (LAI) once a month and Tf once a week in 25 grids placed in a Moso bamboo (Phyllostachys pubescens) forest for 1 year. The mean LAI and spatial variation in LAI did have some seasonal variations. The spatial variations in Tf reduced with increasing Rf, and the relationship between the spatial variation and the Rf held throughout the year. These results indicate that the seasonal change in LAI had little impact on spatial variations in Tf, and that Rf is a critical factor determining the spatial variations in Tf at the study site. We evaluated potential errors in stand‐scale Tf estimates on the basis of measured Tf data using Monte Carlo sampling. The results showed that the error decreases greatly with increasing sample size when the sample size was less than ∼8, whereas it was near stable when the sample size was 8 or more, regardless of Rf. A sample size of eight results in less than 10% error for Tf estimates based on Student's t‐value analysis and would be satisfactory for interception loss estimates when considering errors included in Rf data. Copyright © 2009 John Wiley & Sons, Ltd.
The tale of transforming growth factor‐beta (TGFβ) signaling: A soigné enigma
Authors: Chaudhury Arindam , Howe Philip H. 
Published: September 2009

AbstractTransforming growth factor‐beta (TGFβ) is a secreted cytokine, which intricately controls a plethora of physiological and pathological processes during development and carcinogenesis. TGFβ exerts antiproliferative effects and functions as a tumor suppressor during early stages of tumorigenesis, whereas at later stages it functions as a tumor promoter aiding in metastatic progression through an autocrine TGFβ loop. Intricate knowledge of TGFβ signaling and its regulation are still evolving. In this review, we make an attempt to showcase the associated enigma of TGFβ signaling in its dual functional role as tumor suppressor and metastatic promoter during early and late stages of carcinogenesis, respectively. © 2009 IUBMB IUBMB Life, 61(10): 929–939, 2009