bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum concentrations of legacy, alternative, and precursor per- and polyfluoroalkyl substances: a descriptive analysis of adult female participants in the MIREC-ENDO study
Authors: Borghese M. M. , Ward A. , MacPherson S. , Manz K. E. , Atlas E. , Fisher M. , Arbuckle T. E. , Braun J. M. , Bouchard M. F. , Ashley-Martin J. 
Published: June 2024

Abstract
Background
Several legacy and emerging per- and polyfluoroalkyl substances (PFAS) have been regulated around the world. There is growing concern over the proliferation of alternative PFAS, as well as PFAS precursors. Biomonitoring data for PFAS are critical for assessing exposure and human health risk.

Methods
We collected serum samples from 289 adult female participants in a 2018–2021 follow-up study of the Maternal-Infant Research on Environmental Chemicals (MIREC) Canadian pregnancy cohort. Samples were analyzed for 40 PFAS using ultra-performance liquid chromatography–tandem mass spectrometry. For those compounds with > 50% detection, as well as the sum of these compounds, we describe serum concentrations and patterns of exposure according to sociodemographic and obstetrical history characteristics.

Results
17 out of 40 PFAS were detected in > 50% of samples with 7 of these detected in > 97% of samples. Median [95th percentile] concentrations (µg/L) were highest for PFOS (1.62 [4.56]), PFOA (0.69 [1.52]), PFNA (0.38 [0.81]), and PFHxS (0.33 [0.92]). Geometric mean concentrations of PFOA and PFHxS were approximately 2-fold lower among those with more children (≥ 3 vs. 1), greater number of children breastfed (≥ 3 vs. ≤ 1), longer lifetime duration of breastfeeding (> 4 years vs. ≤ 9 months), and shorter time since last pregnancy (≤ 4 years vs. > 8 years). We observed similar patterns for PFOS, PFHpS, and the sum of 17 PFAS, though the differences between groups were smaller. Concentrations of PFOA were higher among “White” participants, while concentrations of N-MeFOSE, N-EtFOSE, 7:3 FTCA, and 4:2 FTS were slightly higher among participants reporting a race or ethnicity other than “White”. Concentrations of legacy, alternative, and precursor PFAS were generally similar across levels of age, education, household income, body mass index, and menopausal status.

Conclusions
We report the first Canadian biomonitoring data for several alternative and precursor PFAS. Our findings suggest that exposure to PFAS, including several emerging alternatives, may be widespread. Our results are consistent with previous studies showing that pregnancy and breastfeeding are excretion pathways for PFAS.
Cell surface CD55 traffics to the nucleus leading to cisplatin resistance and stemness by inducing PRC2 and H3K27 trimethylation on chromatin in ovarian cancer
Authors: Bharti Rashmi , Dey Goutam , Khan Debjit , Myers Alex , Huffman Olivia G. , Saygin Caner , Braley Chad , Richards Elliott , Sangwan Naseer , Willard Belinda , Lathia Justin D. , Fox Paul L. , Lin Feng , Jha Babal Kant , Brown J. Mark , Yu Jennifer S. , Dwidar Mohammed , Joehlin-Price Amy , Vargas Roberto , Michener Chad M. , Longworth Michelle S. , Reizes Ofer 
Published: June 2024

Abstract
Background
Platinum resistance is the primary cause of poor survival in ovarian cancer (OC) patients. Targeted therapies and biomarkers of chemoresistance are critical for the treatment of OC patients. Our previous studies identified cell surface CD55, a member of the complement regulatory proteins, drives chemoresistance and maintenance of cancer stem cells (CSCs). CSCs are implicated in tumor recurrence and metastasis in multiple cancers.

Methods
Protein localization assays including immunofluorescence and subcellular fractionation were used to identify CD55 at the cell surface and nucleus of cancer cells. Protein half-life determinations were used to compare cell surface and nuclear CD55 stability. CD55 deletion mutants were generated and introduced into cancer cells to identify the nuclear trafficking code, cisplatin sensitivity, and stem cell frequency that were assayed using in vitro and in vivo models. Detection of CD55 binding proteins was analyzed by immunoprecipitation followed by mass spectrometry. Target pathways activated by CD55 were identified by RNA sequencing.

Results
CD55 localizes to the nucleus of a subset of OC specimens, ascites from chemoresistant patients, and enriched in chemoresistant OC cells. We determined that nuclear CD55 is glycosylated and derived from the cell surface pool of CD55. Nuclear localization is driven by a trafficking code containing the serine/threonine (S/T) domain of CD55. Nuclear CD55 is necessary for cisplatin resistance, stemness, and cell proliferation in OC cells. CD55 S/T domain is necessary for nuclear entry and inducing chemoresistance to cisplatin in both in vitro and in vivo models. Deletion of the CD55 S/T domain is sufficient to sensitize chemoresistant OC cells to cisplatin. In the nucleus, CD55 binds and attenuates the epigenetic regulator and tumor suppressor ZMYND8 with a parallel increase in H3K27 trimethylation and members of the Polycomb Repressive Complex 2.

Conclusions
For the first time, we show CD55 localizes to the nucleus in OC and promotes CSC and chemoresistance. Our studies identify a therapeutic mechanism for treating platinum resistant ovarian cancer by blocking CD55 nuclear entry.
Tumor-specific enhanced NIR-II photoacoustic imaging via photothermal and low-pH coactivated AuNR@PNIPAM-VAA nanogel
Authors: Sun Xiaodong , Li Yujie , Liu Xiaowan , Cui Dandan , Shi Yujiao , Huang Guojia 
Published: June 2024

Abstract
Background
Properly designed second near-infrared (NIR-II) nanoplatform that is responsive tumor microenvironment can intelligently distinguish between normal and cancerous tissues to achieve better targeting efficiency. Conventional photoacoustic nanoprobes are always “on”, and tumor microenvironment-responsive nanoprobe can minimize the influence of endogenous chromophore background signals. Therefore, the development of nanoprobe that can respond to internal tumor microenvironment and external stimulus shows great application potential for the photoacoustic diagnosis of tumor.

Results
In this work, a low-pH-triggered thermal-responsive volume phase transition nanogel gold nanorod@poly(n-isopropylacrylamide)-vinyl acetic acid (AuNR@PNIPAM-VAA) was constructed for photoacoustic detection of tumor. Via an external near-infrared photothermal switch, the absorption of AuNR@PNIPAM-VAA nanogel in the tumor microenvironment can be dynamically regulated, so that AuNR@PNIPAM-VAA nanogel produces switchable photoacoustic signals in the NIR-II window for tumor-specific enhanced photoacoustic imaging. In vitro results show that at pH 5.8, the absorption and photoacoustic signal amplitude of AuNR@PNIPAM-VAA nanogel in NIR-II increases up obviously after photothermal modulating, while they remain slightly change at pH 7.4. Quantitative calculation presents that photoacoustic signal amplitude of AuNR@PNIPAM-VAA nanogel at 1064 nm has ~ 1.6 folds enhancement as temperature increases from 37.5 °C to 45 °C in simulative tumor microenvironment. In vivo results show that the prepared AuNR@PNIPAM-VAA nanogel can achieve enhanced NIR-II photoacoustic imaging for selective tumor detection through dynamically responding to thermal field, which can be precisely controlled by external light.

Conclusions
This work will offer a viable strategy for the tumor-specific photoacoustic imaging using NIR light to regulate the thermal field and target the low pH tumor microenvironment, which is expected to realize accurate and dynamic monitoring of tumor diagnosis and treatment.
A low-calorie meal replacement improves body composition and metabolic parameters in shift workers with overweight and obesity: a randomized, controlled, parallel group trial
Authors: Sooriyaarachchi Piumika , Jayawardena Ranil , Pavey Toby , King Neil A. 
Published: June 2024

Abstract
Background
Shift work has been identified as a risk factor for several chronic health conditions including obesity. This study evaluated the impact of a low-calorie meal replacement (MR) as a dinner substitute on body composition and metabolic parameters in shift workers with overweight and obesity.

Methods
An 8-week parallel, randomized controlled trial was conducted on overweight and obese shift workers in a large hospital. An intervention group (IG) (n = 25) was provided with a low-calorie MR shake (∼200 kcal) as a replacement for dinner, every day for 8 weeks, while the control group (CG) (n = 25) continued their habitual diet. Anthropometric measurements, body composition, biochemical, and lifestyle data were assessed at the first and last visits. Analyses were done per protocol (PP) and by intention to treat (ITT).

Results
Over the study duration, both groups displayed moderate changes in anthropometric measurements and body composition, although these were not statistically significant according to the PP analysis. In the ITT analysis, apart from the hip circumference (HC), all other anthropometric parameters demonstrated significant group and time interactions, suggesting the advantageous effects of the meal replacement over the study period (P < 0.05). HDL and VLDL cholesterol measures showed significant main effects, influenced by both group (P = 0.031) and time (P = 0.050) respectively. The most pronounced dietary shift in the IG was a reduction in carbohydrate consumption and an increase in protein intake. Throughout the study, the meal replacement was well-tolerated, with no adverse events reported.

Conclusions
The meal replacement dietary intervention appears to offer beneficial health effects over time. Extended research is crucial to understand the broader implications of meal replacements across diverse populations.

Trial registration
Australian New Zealand Clinical Trials Registry (ANZCTR): ACTRN12622000231741. Registered on 09 February 2022. https://www.anzctr.org.au/ACTRN12622000231741.aspx.
Exploration of new space elicits phosphorylation of GluA1(Ser831) and S6K and expression of Arc in the hippocampus in vivo as in long-term potentiation
Authors: Cagnetta Roberta , Lacaille Jean-Claude , Sonenberg Nahum 
Published: June 2024

AbstractThe brain responds to experience through modulation of synaptic transmission, that is synaptic plasticity. An increase in the strength of synaptic transmission is manifested as long-term potentiation (LTP), while a decrease in the strength of synaptic transmission is expressed as long-term depression (LTD). Most of the studies of synaptic plasticity have been carried out by induction via electrophysiological stimulation. It is largely unknown in which behavioural tasks such synaptic plasticity occurs. Moreover, some stimuli can induce both LTP and LTD, thus making it difficult to separately study the different forms of synaptic plasticity. Two studies have shown that an aversive memory task – inhibitory avoidance learning and contextual fear conditioning – physiologically and selectively induce LTP and an LTP-like molecular change, respectively, in the hippocampus in vivo. Here, we show that a non-aversive behavioural task – exploration of new space – physiologically and selectively elicits a biochemical change in the hippocampus that is a hallmark of LTP. Specifically, we found that exploration of new space induces an increase in the phosphorylation of GluA1(Ser831), without affecting the phosphorylation of GluA1(Ser845), which are biomarkers of early-LTP and not NMDAR-mediated LTD. We also show that exploration of new space engenders the phosphorylation of the translational regulator S6K and the expression of Arc, which are features of electrophysiologically-induced late-LTP in the hippocampus. Therefore, our results show that exploration of new space is a novel non-aversive behavioural paradigm that elicits molecular changes in vivo that are analogous to those occurring during early- and late-LTP, but not during NMDAR-mediated LTD.
Convergence of Fibonacci–Ishikawa iteration procedure for monotone asymptotically nonexpansive mappings
Authors: Alam Khairul Habib , Rohen Yumnam , Saleem Naeem , Aphane Maggie , Rzzaque Asima 
Published: June 2024

AbstractIn uniformly convex Banach spaces, we study within this research Fibonacci–Ishikawa iteration for monotone asymptotically nonexpansive mappings. In addition to demonstrating strong convergence, we establish weak convergence result of the Fibonacci–Ishikawa sequence that generalizes many results in the literature. If the norm of the space is monotone, our consequent result demonstrates the convergence type to the weak limit of the sequence of minimizing sequence of a function. One of our results characterizes a family of Banach spaces that meet the weak Opial condition. Finally, using our iterative procedure, we approximate the solution of the Caputo-type nonlinear fractional differential equation.
Chemical stimulation of geothermal reservoirs using retarded acid systems: current developments and potential directions
Authors: Grifka Jasmin , Licha Tobias , Heinze Thomas 
Published: June 2024

AbstractStimulation techniques to enhance fluid pathways are an important tool to make geothermal projects economically feasible. So far, hydraulic stimulation is used almost exclusively for reservoir-wide improvement of the permeability, but induced seismicity poses a challenge. Chemical stimulation on the other hand has been limited to the close vicinity of the borehole and has barely been considered for the creation of enhanced geothermal reservoirs. However, retardation mechanisms reducing the chemical reaction rate can be used to increase the radius of the chemical stimulation thus enabling a reservoir-wide enhancement of fluid pathways. In this work, we review the technologies of retardation mechanisms for chemical stimulation in geothermal systems and identify five groups of retardation techniques: (i) causing impaired mobility of the acid, e.g., by gelling agents; (ii) causing an impaired dissociation, e.g., by the in-situ generation of the reactive compounds; (iii) blocking the mineral surface area, e.g., by alternating injections of pad fluids and acids; (iv) reducing the reaction rate constant, e.g., by cooling; and (v) changing the chemical equilibrium through chelating agents. We found that most applications are currently based on the use of impaired dissociation, but present research focuses on the development and application of chelating agents. Most of these retardation techniques are adopted from the hydrocarbon industry, but there are several techniques that have not been applied in the geothermal context so far for various reasons. We identify a distinctive lack of in-depth descriptions of the retardation techniques in various studies—mostly to protect intellectual property. However, in the light of public concern regarding fracking techniques and to independently assess potential environmental hazards, scientific examination of proposed techniques is indispensable.
A comprehensive review of deep borehole heat exchangers (DBHEs): subsurface modelling studies and applications
Authors: Kolo Isa , Brown Christopher S. , Nibbs William , Cai Wanlong , Falcone Gioia , Nagel Thomas , Chen Chaofan 
Published: June 2024

AbstractDeep borehole heat exchangers (DBHEs) with depths exceeding 500 m have been researched comprehensively in the literature, focusing on both applications and subsurface modelling. This review focuses on conventional (vertical) DBHEs and provides a critical literature survey to analyse (i) methodologies for modelling; (ii) results from heat extraction modelling; (iii) results from modelling deep borehole thermal energy storage; (iv) results from heating and cooling models; and (v) real case studies. Numerical models generally compare well to analytical models whilst maintaining more flexibility, but often with increased computational resources. Whilst in-situ geological parameters cannot be readily modified without resorting to well stimulation techniques (e.g. hydraulic or chemical stimulation), engineering system parameters (such as mass flow rate of the heat transfer fluid) can be optimised to increase thermal yield and overall system performance, and minimise pressure drops. In this active research area, gaps remain, such as limited detailed studies into the effects of geological heterogeneity on heat extraction. Other less studied areas include: DBHE arrays, boundary conditions and modes of operation. A small number of studies have been conducted to investigate the potential for deep borehole thermal energy storage (BTES) and an overview of storage efficiency metrics is provided herein to bring consistency to the reporting of thermal energy storage performance of such systems. The modifications required to accommodate cooling loads are also presented. Finally, the active field of DBHE research is generating a growing number of case studies, particularly in areas with low-cost drilling supply chains or abandoned hydrocarbon or geothermal wells suitable for repurposing. Existing and planned projects are thus presented for conventional (vertical) DBHEs. Despite growing interest in this area of research, further work is needed to explore DBHE systems for cooling and thermal energy storage.
Nail fold capillaroscopy as a potential tool to evaluate breast tumor
Authors: Kim Minsuk 
Published: June 2024

AbstractIt is necessary to verify whether nail fold capillaroscopy can be utilized for the early detection of breast cancer. To establish this technology, an animal model was developed, utilizing mice for nail fold observations. Nail fold capillaroscopy revealed a human-like anatomical pattern and facilitated the observation of cellular movement within blood vessels. Injection of MCF-7 or mammary fibroblasts in mice allowed the observation of cellular vibrations using motion microscopy from nail fold. We have named this technology ‘capillary cell motion microscopy.’ Intriguingly, we were able to identify distinct cellular vibrations in the MCF-7 group. Moreover, evaluating its effectiveness in mice with chemically induced cancer revealed higher sensitivity (81%-85%) compared to conventional methods (45%-68%). Capillary cell motion microscopy, operating at 0.5–1.5 Hz, provided clear distinction of tumor cells and demonstrated potential applicability in human subjects. While condition adjustments may be necessary, this method holds promise for noninvasive breast cancer detection through nail fold observations.
Detection of bladder tumor cells using motion features in urine
Authors: Kim Minsuk 
Published: June 2024

AbstractUrinary exfoliated tumor cells have emerged as promising biomarkers for predicting, diagnosing, and guiding therapy in bladder cancer. Several methodologies based on biological and physical differences between normal cells and malignant tumor cells have been developed over the past few years. However, these methods still did not have sufficient sensitivity or specificity. In this study, a remote analysis protocol was devised utilizing motion microscopy. This technique amplifies vibrations within a recorded video by re-rendering motions, thereby generating highly magnified visuals. This approach aims to detect dynamic motions that may not be perceptible to the human eye under normal observation. Remarkably, motion microscopy unveiled discernible fluctuations surrounding bladder malignant tumor cells, which we referred to herein as cellular trail. The cellular trails were predominantly evident at around 1 Hz in amplified video images, with a velocity of 22 μm/s. Moreover, cellular trails were observed regardless of whether they were in a non-Newtonian or Newtonian fluid environment. Significantly, this phenomenon was distinguishable even in urine samples. In conclusion, we suggest motion microscopy as an innovative approach for detecting urinary malignant tumors with potential clinical utility as a complementary tool to cytology.
Evaluating a grant development public involvement funding scheme: a qualitative document analysis
Authors: Foster Alexis , Caunt Sharon , Schofield Holly , Glerum–Brooks Karen , Begum Samina , Gleeson Phil , Prestwich Graham , Baird Wendy 
Published: June 2024

Abstract
Background
Undertaking Patient and Public Involvement (PPI) when developing health and social care research grant applications is critical. However, researchers may not have any funding to undertake PPI when developing grants. In response, the National Institute for Health and Care Research- Research Design Service for Yorkshire and the Humber in the United Kingdom, provided Public Involvement Fund Awards of up to £600 to fund PPI activity when researchers were developing grant applications. Researchers provided post-activity reports about how they utilised the Public Involvement Fund. These reports were analysed with the aim of evaluating the usefulness of the Public Involvement Fund and to provide learning about supporting researchers to undertake PPI when developing grants.

Methods
The project was a qualitative document analysis of 55 reports. Initially a researcher coded four reports and three Public Contributors provided feedback. Researchers coded the remaining reports and identified key findings. A workshop was held with the three Public Contributors to develop the findings.

Results
Researchers accessing the Public Involvement Fund award were generally early career researchers or clinicians who did not have other sources of funding for pre-grant PPI input. Researchers felt the award was useful in enabling them to conduct PPI, which strengthened their grant applications. Some researchers found that the award limit of £600 and guidance encouraging expenditure within three months, made it difficult to undertake PPI throughout the full grant development process. Instead, the majority of researchers consulted Public Contributors on one or two occasions. Researchers struggled to recruit diverse members or run group sessions due to the time pressures of grant deadlines. Researchers wanted training on undertaking PPI alongside the financial support.

Conclusions
Researchers, especially early career researchers found having a Public Involvement Fund award instrumental in enabling them to undertake PPI when developing grant applications. It would be beneficial for similar schemes to be widely available. Schemes need to provide sufficient funding to enable meaningful PPI and allow researchers to hold the award for long enough to facilitate involvement during the whole grant development process. Researchers continue to need training on undertaking PPI.