bluuebunny/crossref_metadata_2025_split huggingface dataset:
Embryo‐fetal erythroid megaloblasts in the human coelomic cavity
Authors: Renda Maria Concetta , Giambona Antonino , Fecarotta Emanuela , Leto Filippo , Makrydimas George , Renda Disma , Damiani Gianfranca , Jakil Maria Cristina , Picciotto Francesco , Piazza Angela , Valtieri Mauro , Maggio Aurelio 
Published: June 2010

AbstractThe coelomic cavity is part of the extraembryonic mesoderm, surrounding amniotic cavity, embryo, and yolk sac in the early gestation. It is now believed to represent an important transfer interface and a reservoir of nutrients for the embryo. Coelocentesis by ultrasound‐guided transvaginal puncture offers an easier access to the early human embryo, from 28 days post‐fertilization. However, despite some studies about its biochemical composition being reported, our knowledge about the presence of cellular elements and their quality in this compartment are still limited. Here we studied human coelomic fluids sampled from 6.6 (48 days) to 10 weeks of gestation, demonstrating the presence of functional embryonic erythroid precursors, that is, megaloblasts in the coelomic cavity. The ease of access of the coelomic cavity could allow the development of novel strategies for diagnostic or therapeutic purposes by ultrasound imaging and ultrasound‐guided puncture. J. Cell. Physiol. 225: 385–389, 2010. © 2010 Wiley‐Liss, Inc.
The effect of cell density on the maturation and contractile ability of muscle derived cells in a 3D tissue‐engineered skeletal muscle model and determination of the cellular and mechanical stimuli required for the synthesis of a postural phenotype
Authors: Mudera V. , Smith A.S.T. , Brady M.A. , Lewis M.P. 
Published: June 2010

AbstractThe successful engineering of a truly biomimetic model of skeletal muscle could have a significant impact on a number of biomedical disciplines. Although a variety of techniques are currently being developed, there is, as of yet, no widely available and easily reproducible culture system for the synthesis of 3D artificial muscle tissues. In attempting to generate such a model it is essential to optimise any protocol in order to generate a tissue that best represents the in vivo environment. Since the maturation of muscle derived cells in culture is critically dependent on density, a major factor to be addressed in the development of these models is the ideal concentration at which to seed cells in order to generate an optimal response. In studying the effect of cell density on the performance of cells in an established 3D collagen based model of skeletal muscle, we demonstrate that an optimum density does exist in terms of peak force generation and myogenic gene expression data. Greater densities however, lead to the formation of a more physiologically relevant tissue with a phenotype characteristic of slow, postural muscle. J. Cell. Physiol. 225: 646–653, 2010. © 2010 Wiley‐Liss, Inc.
Rural out‐migration and economic development at origin: A review of the evidence
Authors: Mendola Mariapia 
Published: June 2010

AbstractA major feature of the current ‘mass migration’ process is its strong linkages to countries of origin. Migrants belong to spatially extended families and play a crucial role in shaping economic development in home regions. This paper reviews the wide‐ranging socio‐economic literature on the nexus between labor migration, both domestic and international, and economic development at origin, with a special focus on out‐migration from poor rural regions of developing countries. We disentangle direct effects on migrant‐sending households from spillover effects on the rest of the economy, highlighting some key knowledge gaps and policy concerns related to the complex and intimate relation between rural labour mobility and economic behaviour of people left behind. Copyright © 2010 John Wiley & Sons, Ltd.
The weekend matters: Relationships between stress recovery and affective experiences
Authors: Fritz Charlotte , Sonnentag Sabine , Spector Paul E. , McInroe Jennifer A. 
Published: June 2010

AbstractNon‐work experiences during the weekend provide opportunities to recover from work demands and to replenish lost resources. This longitudinal study examined how specific recovery experiences during the weekend (relaxation, mastery, control, and detachment), as well as non‐work hassles, were associated with specific positive and negative affective states during the following workweek. Participants (N = 229) completed surveys before the weekend, during the weekend, and during the following workweek. Hierarchical regression analyses revealed that after controlling for affective states the previous week, recovery experiences during the weekend significantly explained variance in affective states at the end of the weekend and during the following workweek. Suggestions for future research include a closer examination of the role of individual differences, self‐regulation, and specific work demands in employee stress recovery. Copyright © 2010 John Wiley & Sons, Ltd.
Who and what is fair matters: A multi‐foci social exchange model of creativity
Authors: Khazanchi Shalini , Masterson Suzanne S. 
Published: June 2010

AbstractTo better understand the role of fairness in creativity, we developed and tested a multi‐foci social exchange model of creativity. Specifically, we examined the effect of both the organization and supervisor as distinct sources of interpersonal and informational justice types on creativity through the mediating mechanisms of trust and social exchange relationships. The data were collected from 205 employees and their supervisors at a large chemical engineering plant in India. Structural equation modeling was used to test the model. Results show that both interpersonal and informational justice types influence creativity via different mechanisms but both justice types were not equally important for employee creativity. Copyright © 2010 John Wiley & Sons, Ltd.
Genetic underpinnings of survey response
Authors: Thompson Lori Foster , Zhang Zhen , Arvey Richard D. 
Published: June 2010

AbstractThis study investigates the influence of genetic factors on survey response behavior. A pool of 558 male and 500 female twin pairs from the Minnesota Twin Registry (MTR) was asked to complete a paper‐and‐pencil survey of leadership activities. We used quantitative genetics techniques to estimate the genetic, shared environmental, and nonshared environmental effects on people's compliance with the request for survey participation. Results indicated that genetic influences explained 45% of the variance in survey response behavior for both women and men, with little shared environmental effects. Similar estimates were obtained after we partialled out potential confounds including twin closeness, age, and education. The results have important implications for response rates and nonresponse bias in survey‐based research. Copyright © 2010 John Wiley & Sons, Ltd.
The integrated WRF/urban modelling system: development, evaluation, and applications to urban environmental problems
Authors: Chen Fei , Kusaka Hiroyuki , Bornstein Robert , Ching Jason , Grimmond C. S. B. , Grossman‐Clarke Susanne , Loridan Thomas , Manning Kevin W. , Martilli Alberto , Miao Shiguang , Sailor David , Salamanca Francisco P. , Taha Haider , Tewari Mukul , Wang Xuemei , Wyszogrodzki Andrzej A. , Zhang Chaolin 
Published: June 2010

AbstractTo bridge the gaps between traditional mesoscale modelling and microscale modelling, the National Center for Atmospheric Research, in collaboration with other agencies and research groups, has developed an integrated urban modelling system coupled to the weather research and forecasting (WRF) model as a community tool to address urban environmental issues. The core of this WRF/urban modelling system consists of the following: (1) three methods with different degrees of freedom to parameterize urban surface processes, ranging from a simple bulk parameterization to a sophisticated multi‐layer urban canopy model with an indoor–outdoor exchange sub‐model that directly interacts with the atmospheric boundary layer, (2) coupling to fine‐scale computational fluid dynamic Reynolds‐averaged Navier–Stokes and Large‐Eddy simulation models for transport and dispersion (T&D) applications, (3) procedures to incorporate high‐resolution urban land use, building morphology, and anthropogenic heating data using the National Urban Database and Access Portal Tool (NUDAPT), and (4) an urbanized high‐resolution land data assimilation system. This paper provides an overview of this modelling system; addresses the daunting challenges of initializing the coupled WRF/urban model and of specifying the potentially vast number of parameters required to execute the WRF/urban model; explores the model sensitivity to these urban parameters; and evaluates the ability of WRF/urban to capture urban heat islands, complex boundary‐layer structures aloft, and urban plume T&D for several major metropolitan regions. Recent applications of this modelling system illustrate its promising utility, as a regional climate‐modelling tool, to investigate impacts of future urbanization on regional meteorological conditions and on air quality under future climate change scenarios. Copyright © 2010 Royal Meteorological Society
Quantitative determination of fluoxetine in human serum by high performance thin layer chromatography
Authors: Mennickent Sigrid , Fierro Ricardo , Vega Mario , De Diego Marta , Godoy C. Gloria 
Published: June 2010

AbstractA high performance thin layer chromatographic method was developed and validated for the quantification of fluoxetine in human serum. Fluoxetine was extracted by liquid–liquid extraction method with diethyl ether as extraction solvent. Imipramine was used as internal standard. The chromatographic separation was achieved on precoated silica gel F 254 high performance thin layer chromatographic plates using a mixture of toluene/acetic acid glacial (4:5 v/v) as mobile phase. 4‐Dimethylamino‐azobenzene‐4‐sulphonyl chloride was used as derivatization reagent. Densitometric detection was done at 272 nm. The method was linear between 12.5 and 87.5 ng/spot, corresponding to 0.05 and 0.35 ng/μL of fluoxetine in human serum after extraction process and applying 25 μL to the chromatographic plates. The method correlation coefficient was 0.999. The intra‐assay and inter‐assay precisions, expressed as the RSD, were in the range of 0.70–2.01% (n=3) and 0.81–3.90% (n=9), respectively. The LOD was 0.23 ng, and the LOQ was 0.70 ng. The method proved be accurate, with a recovery between 94.75 and 98.95%, with a RSD not higher than 3.61% and was selective for the active principle tested. This method was successfully applied to quantify fluoxetine in patient serum samples. In conclusion, the method is useful for quantitative determination of fluoxetine in human serum.
Enzymatic transformation of platycosides and one‐step separation of platycodin D by high‐speed countercurrent chromatography
Authors: Ha In Jin , Ha Young Wan , Kang Minseok , Lee Jongsung , Park Deokhoon , Kim Yeong Shik 
Published: June 2010

AbstractPlatycosides, the saponins found in the roots of Platycodon grandiflorum (Platycodi Radix), are typically composed of oleanane triterpenes with two side chains. In platycosides, platycodin D, a glucose unit at C‐3, is a major component, which has several pharmacological activities. Because of the high demand for this compound, we attempted to enzymatically convert platycodin D3 and platycoside E, having two and three glucose units at C‐3, respectively, into platycodin D. In this study, we tested the ability of several glycosidases to transform platycosides, or more specifically, the ability to transform platycoside E and platycodin D3 into platycodin D. To obtain pure platycodin D on a preparative scale, high‐speed countercurrent chromatography with a solvent system of ethyl acetate/n‐butanol/water (1.2:1:2, v/v/v) was used for the separation of the enzymatically transformed product. Approximately 39.4 mg of platycodin D (99.8% purity) was obtained from 200 mg of the product in a one‐step separation. The results strongly support the advantage of enzymatic transformation of the platycosides for the efficient enrichment of platycodin D in the complicated extract of the medicinal plant.
Investigation of ractopamine‐imprinted polymer for dispersive solid‐phase extraction of trace β‐agonists in pig tissues
Authors: Hu Yuling , Liu Ruijin , Li Yuanwen , Li Gongke 
Published: June 2010

AbstractRactopamine, as an alternative β‐agonist to clenbuterol, is more and more used as leanness‐enhancing agent in the swine industry. This work presents a new molecularly imprinted polymer (MIP) using ractopamine as template for dispersive solid‐phase extraction of trace ractopamine and the structural related β‐agonists in animal tissues. The binding properties and selectivity of MIP were investigated. High selectivity in polar environment was found, since the extraction capacity of ractopamine with the MIP was 4.5‐fold as much as that with the non‐imprinted polymer in acetonitrile. Cross‐selectivity investigation indicates that the MIP preferentially binds the template and then the structural analogues according to their molecular similarity. Thermodynamic and kinetic investigation was performed to interpret the specific adsorption and molecular recognition of the MIP for ractopamine. Standard free energy, standard enthalpy, and standard entropy were determined. Related information suggested that adsorption of ractopamine onto MIP was an exothermic, spontaneous process. The MIP can be applied as dispersive solid‐phase extraction material for enrichment of ractopamine, isoxsuprine, fenoterol and clenbuterol in complex samples before HPLC analysis. The method revealed detection limits of 0.20–0.90 μg/L, recoveries of 83.8–115.2 and 85.2–110.2% for the spiked pig muscle and pig liver, respectively, with the RSD from 2.5 to 8.8%.
Optimization of microwave‐assisted extraction of protopine and allocryptopine from stems of Macleaya cordata (Willd) R. Br. using response surface methodology
Authors: Zhong Ming , Huang Ke‐Long , Zeng Jian‐Guo , Li Shuang , She Jin‐Ming , Li Guiyin , Zhang Li 
Published: June 2010

AbstractThe purpose of the research was to investigate the multiple response optimizations for the extraction of protopine and allocryptopine from the stems of Macleaya cordata (Willd) R. Br. by using microwave‐assisted extraction (MAE). A three‐level, three‐factor Box–Behnken design of response surface methodology was used to develop response model, and desirability function was employed to optimize the effects of main extraction parameters. Three variables, ethanol concentration (20–80%, v/v), extraction temperature (30–70°C) and solvent/solid ratio (10:1 to 30:1, mL/g), were investigated in this study. The results showed that the optimum parameters of MAE were ethanol concentration of 45.2 % (v/v), extraction temperature of 54.7°C and solvent/solid ratio of 20.4:1 (mL/g). Under these conditions, the extraction yields of protopine and allocryptopine were 89.4 and 102.0%, respectively, and the extracta sicca yield was 12.5%. The combination use of response surface methodology, Box‐Behnken design and the appropriate desirability function could provide an insight into a lab‐scale MAE process, and help to develop procedures for commercial production of active ingredients from medical plants.
A rapid method with ultra‐high‐performance liquid chromatography–tandem mass spectrometry for simultaneous determination of five type B trichothecenes in traditional Chinese medicines
Authors: Han Zheng , Liu Xuesong , Ren Yiping , Luan Lianjun , Wu Yongjiang 
Published: June 2010

AbstractA speedy and selective ultra‐HPLC‐MS/MS method for simultaneous determination of deoxynivalenol (DON), 3‐acetyldeoxynivalenol (3‐ADON), 15‐ADON, nivalenol and fusarenon X in traditional Chinese medicines (TCMs) was developed. The method was based on one‐step sample cleanup using reliable homemade cleanup cartridges. A linear gradient mobile‐phase system, consisting of water containing 0.2% aqueous ammonia and acetonitrile/methanol (90:10, v/v) at a flow rate of 0.4 mL/min, and an Acquity UPLC HSS T3 column (100 mm×2.1 mm, 1.8 μm) were employed to obtain the best resolution of the target analytes. [13C15]–DON was used as the internal standard to accomplish as accurate as possible quantitation. The established method was further validated by determining the linearity (R2≥0.9990), sensitivity (LOQ, 0.29–0.99 μg/kg), recovery (88.5–119.5%) and precision (RSD≤15.8%). It was shown to be a suitable method for simultaneous determination of DON, 3‐ADON, 15‐ADON, nivalenol and fusarenon X in various TCM matrices. The utility and practical impact of the method was demonstrated using different TCM samples.
Ultra‐performance LC MS/MS method for quantification of clopidogrel active metabolite
Authors: Delavenne Xavier , Basset Thierry , Zufferey Paul , Malouk Nora , Laporte Silvy , Mismetti Patrick 
Published: June 2010

AbstractA sensible ultra‐performance LC–MS method was developed and validated for the quantification of clopidogrel active metabolite in human plasma, with clopidogrel D4 as internal standard. Plasma pretreatment involved a one‐step protein precipitation with acetonitrile. The separation was performed by reverse‐phase chromatography on a C8 column. The method was linear over the concentration range of 1–150 ng/mL. The intra‐ and inter‐day precision values were below 17% and accuracy was from 1.7 to 7.5% at all quality control levels. The lower LOQ was 0.8 ng/mL. Sample analysis time was reduced to 5 min including sample preparation (limited to protein precipitation). The present method was successfully applied to a clopidogrel active metabolite pharmacokinetic study following oral administration to healthy volunteers.
Determination of trace organophosphorus pesticides in water samples with TiO2 nanotubes cartridge prior to GC‐flame photometric detection
Authors: Huang Yunrui , Zhou Qingxiang , Xiao Junping , Xie Guohong 
Published: June 2010

AbstractThis article described a new method for the sensitive determination of organophosphorus pesticides in water samples using SPE in combination with GC‐flame photometric detection. In the procedure of method development, TiO2 nanotubes were used as SPE adsorbents for the enrichment of organophosphorus pesticides from water samples. Several factors, such as eluent and its volume, sample pH, sample volume, sample flow rate, and concentration of humic acid, were optimized. Under the optimal conditions, the proposed method had good linear ranges as 0.1–40 μg/L for each of them, LOD of 0.11, 0.014, and 0.0025 μg/L, and LOQs of 0.37, 0.047, and 0.0083 μg/L for chlorpyrifos, phorate, and methyl parathion, respectively. The proposed method was validated with real environmental water samples and the spiked recoveries were over the range of 86.5–115.1%. All these results indicated that TiO2 nanotubes, as a new SPE adsorbent, would be used widespread for the preconcentraiton and determination of environmental pollutants in the future.
Capillary isotachophoresis for the analysis of ionic liquid entities
Authors: Markowska Aleksandra , Stepnowski Piotr 
Published: June 2010

AbstractSimple, selective and sensitive isotachophoretic methods for the analysis of ionic liquid (IL) compartments were developed in this study. A leading electrolyte containing 10 mM L‐histidine + 10 mM histidine hydrochloride and a terminating electrolyte containing 5 mM glutamic acid + 5 mM L‐histidine were selected to separate nitrate(V), chlorate(V), hexafluorophosphate, dicyanimide, trifluoromethanesulfonate, phosphate(V) and bis(trifluoromethanesulfonyl)imide in anionic mode. In contrast, seven short‐chain alkylimidazolium, alkylpyrrolidinium, alkylpyridinium and non‐chromophoric tetraalkylammonium and tetraalkylphosphonium IL cations were separated with 10 mM potassium hydroxide + 10 mM acetic acid as leading electrolyte, and 10 mM β‐alanine + 10 mM acetate as terminating electrolyte. Both methods were optimized and validated with good analytical performance parameters. LOD was about 3–5 μM, and the repeatability lay in the range of 1.06–5.59%. These methods were evaluated for their applicability to the analysis of soil samples and freshwater contaminated with ILs. In light of hitherto the absence of reports on the determination of non‐chromophoric IL cations, this study delivers for the first time a universal method enabling analysis of these species. Moreover, as there is still significant lack of methodologies of IL anion analysis, the obtained results offer an interesting alternative in that matter.
Neural stimulation with optical radiation
Authors: Richter C.‐P. , Matic A.I. , Wells J.D. , Jansen E.D. , Walsh J.T. 
Published: June 2010

AbstractThis paper reviews the existing research on infrared neural stimulation, a means of artificially stimulating neurons that has been proposed as an alternative to electrical stimulation. Infrared neural stimulation (INS) is defined as the direct induction of an evoked potential in response to a transient targeted deposition of optical energy. The foremost advantage of using optical radiation for neural stimulation is its spatial resolution. Exogenously applied or trans‐genetically synthesized fluorophores are not used to achieve stimulation. Here, current work on INS is presented for motor nerves, sensory nerves, central nervous system, and in vitro preparations. A discussion follows addressing the mechanism of INS and its potential use in neuroprostheses. A brief review of neural depolarization involving other optical methods is also presented. Topics covered include optical stimulation concurrent with electrical stimulation, optical stimulation using exogenous fluorophores, and optical stimulation by transgenic induction of light‐gated ion channels.
Severe polyuria and polydipsia in hyponatremic‐hypertensive syndrome associated with Wilms tumor
Authors: D'Angelo Paolo , Catania Serena , Zirilli Giusy , Collini Paola , Tropia Serena , Perotti Daniela , Terenziani Monica , Spreafico Filippo 
Published: June 2010

AbstractThe combination of hyponatremia and renovascular hypertension is known as hyponatremic‐hypertensive syndrome (HHS) and so rarely described in children but associated with various kinds of occlusions of the renal artery. We describe two children who presented HHS with severe hypokalemia, polyuria, and polydipsia associated with Wilms tumor, which required treatment with an angiotensin‐converting enzyme inhibitor before nephrectomy. All HHS signs and symptoms resolved only following surgical resection of the tumor, allowing chemotherapy to be given. Pediatr Blood Cancer. 2010;55:566–569. © 2010 Wiley‐Liss, Inc.