bluuebunny/crossref_metadata_2025_split huggingface dataset:
One‐tube HLA‐B27/B2708 typing by flow cytometry using two “Anti‐HLA‐B27” monoclonal antibody reagents
Authors: Darke Chris , Coates Ernest 
Published: August 2009

AbstractBackground: Flow cytometry‐based methods are widely used to detect the ankylosing spondylitis‐associated HLA‐B27/B2708 antigens. However, the generally used “HLA‐B27” monoclonal antibodies (moabs) cross‐react with many HLA specificities, including the common HLA‐B7 antigen. Thus, using two “B27” moabs is highly recommended.Methods: The assay used two “HLA‐B27” reagents, FITC and PE conjugated, respectively and a PE‐Cy5 anti‐CD3 antibody. Assay verification used 51 reference subjects possessing B*2705, B*2702, and B*2708 and a range of cross‐reactive HLA antigens. A total of 1,006 consecutive patients' samples, referred for “HLA‐B27 typing”, were assayed alongside our standard flow cytometry method. A further 12 low frequency HLA‐B*27 specificities were tested. Samples reacting with one “B27” moab only were B*27 allele typed by PCR using sequence‐specific primers.Results: All patient B27/B2708 positives (28.3%) were identified by our one‐tube method which detected B*2705, B*2702, B*2708, and 8/12 other B*27 specificities. It was unaffected by HLA‐B7 and other cross‐reactive antigens but required a minor adjustment, a reduction in the volume of one of the “B27” moabs used, to avoid detecting a minority of HLA‐B57 subjects.Conclusions: Our one‐tube B27/B2708 assay is simple, robust, uses two “B27” moabs for typing precision and security, does not suffer from interference by HLA‐B7 or other cross‐reactive antigens and has the obvious advantage of using a single tube per typing. © 2009 Clinical Cytometry Society
Chondroid syringoma diagnosed by fine needle aspiration cytology
Authors: Kumar Bipin 
Published: August 2009

AbstractChondroid syringoma is a rare benign skin adnexal tumor of eccrine/apocrine origin affecting commonly the head and neck region. It used to be previously called as mixed tumor of skin because of the presence of both the epithelial and mesenchymal components. There are only few case reports describing the fine needle aspiration cytologic features of chondroid syringoma for diagnosis.We studied a 20‐year‐old male case from Taplejung district of Nepal came to Otorhinolaryngology out patient department with the complaints of painless, progressively enlarging swelling on the dorsum of nose. On examination, 2.0 × 2.0 cm, firm, non‐tender swelling was seen on the right side of dorsum of nose. Overlying skin was normal, and the swelling was fixed to the skin but freely mobile over underlying structure. A clinical diagnosis of dermoid cyst was entertained, and the case was subjected to FNAC. FNA yielded mucoid material which on microscopy showed clusters of round cells with moderate to abundant cytoplasm embedded in chondromyxoid ground substance. The nuclei were monomorphic, centrally to eccentrically located and had fine chromatin. On the basis of these cytologic features, a diagnosis of chondroid syringoma was made.We concluded that Chondroid syringoma may be considered as a rare differential diagnosis in the swelling of head and neck region and the diagnosis can be easily confirmed or ruled out by means of fine needle aspiration cytology. Diagn. Cytopathol. 2010. © 2009 Wiley‐Liss, Inc.
Preparation of thyroid FNA material for routine cytology and BRAF testing: A validation study
Authors: Troncone Giancarlo , Cozzolino Immacolata , Fedele Maria , Malapelle Umberto , Palombini Lucio 
Published: August 2009

AbstractV600E BRAF mutation is emerging as an independent marker of papillary thyroid carcinoma aggressive behavior. Papillary thyroid carcinomas harboring this mutation should be extensively resected. However, this requires an unquestionable cytological diagnosis of malignancy. Thus, cytological specimens should be properly handled to provide both morphological and molecular information. Here, we assessed whether our method of preparation of fine‐needle aspiration material is suitable for both tests. A series of 128, routinely performed, fine‐needle aspirations was analyzed. Each nodule was punctured three times. A representative Diff‐Quik smear prepared from the first two passages was evaluated onsite. When microscopy was diagnostic (n = 44), the third needle pass was dedicated to harvest material for BRAF testing; in the remaining cases (n = 84), additional direct smears for cytology were prepared and the remaining material in the needle plus the needle rinsing was collected for BRAF testing. Cellularity was adequate in 126/128 (98%) cases. Cytological diagnoses were inadequate (2%), benign (85%), follicular lesion of undetermined significance (5%), follicular neoplasms (2%), suspicious for malignancy (2%), and malignant (4%). Higher average of extracted DNA concentration was observed in the dedicated pass group (25.9 vs 7.95 ng/μl). However, the rate of successful exon 15 BRAF amplification was similar with (43/44; 97.7%) or without (79/84; 94%) the dedicated pass. Thus, our protocol is suitable for both tests. Whenever necessary BRAF testing may also be performed on the residual samples of thyroid nodules, without interfering with routine cytology. Diagn. Cytopathol. 2010. © 2009 Wiley‐Liss, Inc.
A Macrocyclic Bistriazene and its Complexes with Divalent Metal Ions
Authors: Beck Johannes , Hörner Manfredo , Dittmann Gertrud 
Published: August 2009

AbstractThe diazotisation of 2,2′‐diaminotolane with one equivalent of HNO2 yields the macrocyclic bis(tolanetriazene) (tolaneN3H)2 in moderate yield. As shown by the crystal structure determination, the molecule is essentially planar. After deprotonation, the central cavity is suitable for complexation of transition metal ions. In pyridine solution, the anion (tolaneN3)22– is formed, which reacts with the acetates of Co2+ and Ni2+ to the dark red coloured complexes [M((tolaneN3)2)(py)2]. The metal ions are located in the centre of the macrocycles, coordinated by four N atoms of the two triazenide groups. Two pyridine ligands occupy the axial positions, giving the central atoms a distorted octahedral coordination environment. In the temperature range between 30 and 300 K, both complexes show the expected magnetic properties of magnetically isolated ions with nearly fulfilled Curie behaviour and μeff = 3.40 for NiII and 5.19 for CoII, which is in the high spin state.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Scandium Aminopyridinates: Synthesis, Structure and Isoprene Polymerization
Authors: Döring Christian , Kretschmer Winfried P. , Bauer Tobias , Kempe Rhett 
Published: August 2009

AbstractAlkane elimination reactions of [Sc(CH2SiMe3)3(thf)2] or [Sc(CH2Ph)3(thf)3] with aminopyridines (1a = (2,6‐diisopropylphenyl)‐[6‐(2,4,6‐triisopropylphenyl)pyridin‐2‐yl]amine,1b = [6‐(2,4,6‐triisopropylphenyl)pyridin‐2‐yl]‐(2,4,6‐trimethylphenyl)amine, 1c = (2,6‐diisopropylphenyl)‐[6‐(2,6‐dimethylphenyl)pyridin‐2‐yl]amine) led to selective formation of dialkyl complexes of scandium stabilized by one aminopyridinato (Ap) ligand. The reaction of these compounds with anilinium borate leads to the elimination of one of the two alkyl functions and affords organoscandium cations. The amine elimination reaction of [Sc{N(SiHMe2)2}3(thf)] with the aminopyridine 1a yields the corresponding mono(aminopyridinato) complex. Single‐crystal X‐ray analyses were carried out for [Ap*Sc(CH2Ph)2(thf)] (3a), [Ap*Sc(CH2Ph)(thf)3][B(C6H5)4] (4a) and [Ap*Sc{N(SiHMe2)2}2] (6a) (Ap*‐H = 1a). The aminopyridinato‐stabilized dialkylscandium complexes [ApScR2(thf)] (R = CH2SiMe3, CH2Ph) are initiators for controlled 3,4‐selective isoprene polymerization after activation with perfluorinated tetraphenyl borates. Variation of the polymerization temperature as well as the addition of different alkylaluminium compounds influence the microstructure of the obtained polymer. Bis(dimethylsilyl)amides of scandium polymerize isoprene in the presence of anilinium borate and alkylaluminium compounds with high cis‐1,4‐selectivity. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
An Unusual Anion–π Interaction in an ;rido Organometallic Assembly: Synthesis, First Crystal Structure, and Computational Study (Eur. J. Inorg. Chem. 25/2009)
Authors: Moussa Jamal , Gandon Vincent , Rager Marie Noelle , Malacria Max , Chamoreau Lise‐Marie , Amouri Hani 
Published: August 2009

AbstractThe cover picture shows the molecular structure of the neutral mononuclear iridium complex Cp*Ir(η6‐C6H2O4)(BF2)2 exhibiting an anion–π interaction between CF3SO3 and metallated pentamethylcyclopentadienyl “η‐Cp*Ir”. Computational analysis (see optimized structure) shows that this type of noncovalent interaction can be better described as a combination of anion–π and CH···anion hydrogen‐bonding interactions. Both contributions work in harmony. Details are discussed in the Short Communication by H. Amouri et al. on p. 3703 ff. The background depicts the famous réverbère “street lamp” of Paris, which resembles the iridium complex holding the anion. The lamp shown in the image is located on Île Saint‐Louis, often considered the most romantic part of Paris. The cover picture was designed by H. Amouri.
Power and behavioral approach orientation in existing power relations and the mediating effect of income
Authors: Lammers Joris , Stoker Janka I. , Stapel Diederik A. 
Published: August 2009

AbstractA large number of authors have observed that the experience of power increases behavioral approach tendencies. There are however some important unresolved problems. Predominantly, the literature relies on lab manipulations, priming, and student populations. This has resulted in low face validity. Also, it is unclear what process underlies this effect. A large‐scale survey (N = 3082) reliably measures power among real low‐ and high‐power employees in existing organizations and finds strong support for the effect of power on behavioral approach. Consistent with expectations, this effect is mediated by increased access to resources. We also discuss findings that suggest the shape of this power‐approach effect might be quadratic. Copyright © 2009 John Wiley & Sons, Ltd.
Incorporating uncertainty in gully erosion calculations using the random forests modelling approach
Authors: Kuhnert Petra M. , Henderson Anne‐Kinsey , Bartley Rebecca , Herr Alexander 
Published: August 2009

AbstractGully erosion represents one of the greatest threats to agricultural watersheds around the world and this is exacerbated with continual changes to land practices and changing climatic condition at a temporal scale. Catchment based models which include gully erosion have received increased attention in recent years due to the impact of gully erosion on downstream water quality. These types of models take gully erosion calculated via a deterministic model at a particular spatial location, along with other measures of erosion to provide an end‐of‐catchment load. Although seen as a major contributor to erosion in this type of modelling framework, the error surrounding the gully erosion calculation is ignored in the sediment model. We propose a methodology that investigates the error in the gully erosion model. The methodology uses Random Forests to predict gully density and its associated prediction error across a catchment using environmental variables, which are then incorporated into the gully erosion model. We demonstrate the approach using the Burdekin catchment in Queensland, Australia, where only a very small proportion of gullies (∼0.88%) have been mapped. Our results cast doubt in the predictive ability of models of sediment transportation that use gully erosion where the error is estimated high. Copyright © 2009 John Wiley & Sons, Ltd.
Price discovery in electronic foreign exchange markets: The sterling/dollar market
Authors: Poskitt Russell 
Published: August 2009

AbstractThis study finds that GLOBEX has a marginally lower Hasbrouck, J. (1995) information share than Reuters D3000 in the electronic sterling/dollar foreign exchange market when returns are computed from high frequency data on either midquotes or transaction prices. However, GLOBEX's information share declines sharply when returns are computed from a mixture of GLOBEX transaction prices and Reuters D3000 midquotes. This helps explain why prior studies using this latter methodology report relatively low information shares for GLOBEX in the yen/dollar market. Variations in GLOBEX's information share on an intraday basis can be explained by variations in relative liquidity, spreads and price volatility. © 2009 Wiley Periodicals, Inc. Jrl Fut Mark 30:590–606, 2010
Construction of a 18F‐FDG PET normative database of Japanese healthy elderly subjects and its application to demented and mild cognitive impairment patients
Authors: Iseki Eizo , Murayama Norio , Yamamoto Ryoko , Fujishiro Hiroshige , Suzuki Masaru , Kawano Masato , Miki Shuya , Sato Kiyoshi 
Published: August 2009

AbstractObjectiveTo construct a 18F‐FDG PET normative database of Japanese healthy elderly subjects and to apply it to demented and mild cognitive impairment (MCI) patients.MethodsSeventy‐seven Japanese normal volunteers from 41 to 84 years of age (36 males and 41 females) who underwent clinical, neuropsychological, and MRI examinations were selected. In these subjects, 18F‐FDG PET/CT scans were performed, 18F‐FDG PET images were analyzed using the 3D‐SSP program, and a normative database for cerebral glucose metabolism was constructed. Then, 18F‐FDG PET images from 14 demented and MCI patients were evaluated based on the normative database.ResultsThe 77 healthy elderly subjects were divided into three groups according to their age. In these subjects, the difference in glucose metabolism between males and females was minimal in contrast, glucose metabolism showed a weak reciprocal correlation with aging in several cerebral regions. The 3D‐SSP images of 14 demented and MCI patients based on the age‐matched 18F‐FDG PET normative database showed decreased patterns of glucose metabolism similar to those of previous studies on dementia diseases and MCI.ConclusionsAn age‐matched normative database can be applied to the evaluation of single subjects, and the application of a mixed database of males and females is viable. Normative databases are useful for detecting dementia diseases and their MCI. Copyright © 2009 John Wiley & Sons, Ltd.
General health status and vascular disorders as correlates of late‐life depressive symptoms in a national survey sample
Authors: Stewart Robert , Hirani Vasant 
Published: August 2009

AbstractObjectivesTo investigate the associations between vascular disease, vascular risk factors and depressive symptoms in a national survey sample, the extent to which these associations are accounted for by general health status and the extent to which the association between depressive symptoms and worse general health is accounted for by level of vascular risk.MethodsData were analysed from the Health Survey for England 2005: a nationally representative cross‐sectional population survey comprising 4269 adults aged ≥ 65 living in private households. Data collected included depressive symptoms (10‐item Geriatric Depression Scale), self‐reported general health and vascular disease/risk factors, resting blood pressure and lipid profile.ResultsCase level depressive symptoms were associated with reported previous stroke, ischaemic heart disease and diabetes, as well as with current smoking. These associations were attenuated substantially when adjusted for general health status. On the other hand, the association between worse subjective health and depressive symptoms was not altered following adjustment for vascular disease/risk status.ConclusionWorse general health appears to account for a large part of associations between cardiovascular disorders and depression, although this may represent ‘over‐adjustment’. Cardiovascular disease/risk does not appear to account for much of the association between worse general health and depression. Copyright © 2009 John Wiley & Sons, Ltd.
Smoking and drinking in relation to depressive symptoms among persons with oral cancer or oral epithelial dysplasia
Authors: Morse Douglas E. , Psoter Walter J. , Baek Linda S. , Eisenberg Ellen , Cohen Donald , Cleveland Deborah , Mohit‐Tabatabai Mirseyed , Reisine Susan 
Published: August 2009

AbstractBackground.We examined whether smoking or drinking during or before the diagnosis‐year of oral cancer or oral epithelial dysplasia (OED) was related to “subsequent depression” measured months after the oral diagnosis.Methods.Incident cases of oral cancer or OED were identified via 3 oral pathology laboratories. A telephone‐administered questionnaire included questions on smoking/drinking history through the diagnosis‐year and measured depressive symptoms using the Center for Epidemiologic Studies‐Depression Scale (CES‐D); scores of 16+ indicated clinical depression. “Subsequent depression” was defined as a CES‐D score of 16+, measured at the time of assessment several months after the diagnosis of oral cancer or OED.Results.Patients who smoked during their diagnosis‐year had twice the odds of subsequent depression relative to former/never smokers. Diagnosis‐year (vs never/former) drinking was not associated with depression; however, average alcohol consumption of >1.5 drinks/week was negatively associated with subsequent depression for both diagnosis‐year and ex‐drinkers (past reported drinking) even among heavy drinkers.Conclusion.Our findings suggest that subsequent depression is positively associated with diagnosis‐year smoking and negatively associated with alcohol consumption of >1.5 drinks/week among both diagnosis‐year and ex‐drinkers. © 2009 Wiley Periodicals, Inc. Head Neck, 2010
Abnormal medial temporal activity for bound information during working memory maintenance in patients with schizophrenia
Authors: Luck David , Danion Jean‐Marie , Marrer Corrine , Pham Bich‐Tuy , Gounot Daniel , Foucher Jack 
Published: August 2009

AbstractAlterations of binding in long‐term memory in schizophrenia are well established and occur as a result of aberrant activity in the medial temporal lobe (MTL). In working memory (WM), such a deficit is less clear and the pathophysiological bases remain unstudied. Seventeen patients with schizophrenia and 17 matched healthy controls performed a WM binding task while undergoing functional magnetic resonance imaging. Binding was assessed by contrasting two conditions comprising an equal amount of verbal and spatial information (i.e., three letters and three spatial locations), but differing in the absence or presence of a link between them. In healthy controls, MTL activation was observed for encoding and maintenance of bound information but not for its retrieval. Between‐group comparisons revealed that patients with schizophrenia showed MTL hypoactivation during the maintenance phase only. In addition, BOLD signals correlated with behavioral performance in controls but not in patients with schizophrenia. Our results confirm the major role that the MTL plays in the pathophysiology of schizophrenia. Short‐term and long‐term relational memory deficits in schizophrenia may share common cognitive and functional pathological bases. Our results provide additional information about the episodic buffer that represents an integrative interface between WM and long‐term memory. © 2009 Wiley‐Liss, Inc.
Cannabis and anxiety: a critical review of the evidence
Authors: Crippa José Alexandre , Zuardi Antonio Waldo , Martín‐Santos Rocio , Bhattacharyya Sagnik , Atakan Zerrin , McGuire Philip , Fusar‐Poli Paolo 
Published: August 2009

AbstractBackgroundAnxiety reactions and panic attacks are the acute symptoms most frequently associated with cannabis use. Understanding the relationship between cannabis and anxiety may clarify the mechanism of action of cannabis and the pathophysiology of anxiety. Aims of the present study were to review the nature of the relationship between cannabis use and anxiety, as well as the possible clinical, diagnostic and causal implications.MethodSystematic review of the Medline, PsycLIT and EMBASE literature.ResultsFrequent cannabis users consistently have a high prevalence of anxiety disorders and patients with anxiety disorders have relatively high rates of cannabis use. However, it is unclear if cannabis use increases the risk of developing long‐lasting anxiety disorders. Many hypotheses have been proposed in an attempt to explain these relationships, including neurobiological, environmental and social influences.ConclusionsThe precise relationship between cannabis use and anxiety has yet to be established. Research is needed to fully clarify the mechanisms of such the association. Copyright © 2009 John Wiley & Sons, Ltd.
Automated laser guidance of neuronal growth cones using a spatial light modulator
Authors: Carnegie David J. , Čižmár Tomáš , Baumgartl Jörg , Gunn‐Moore Frank J. , Dholakia Kishan 
Published: August 2009

AbstractThe growth cone of a developing neuron can be guided using a focused infra‐red (IR) laser beam [1]. In previous setups this process has required a significant amount of user intervention to adjust continuously the laser beam to guide the growing neuron. Previously, a system using an acousto‐optical deflector (AOD) has been developed to steer the beam [2]. However, to enhance the controllability of this system, here we demonstrate the use of a computer controlled spatial light modulator (SLM) to steer and manipulate the shape of a laser beam for use in guided neuronal growth. This new experimental setup paves the way to enable a comprehensive investigation into beam shaping effects on neuronal growth and we show neuronal growth initiated by a Bessel light mode. This is a robust platform to explore the biochemistry of this novel phenomenon. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Effects of recombinant neurotoxins on single Na+ channels in isolated rat hippocampal neurons
Authors: Xiang Hui , Wang Lei , Cui Jihong , Du Jianyang , Wang Ke , Xu Anlong 
Published: August 2009

AbstractFour recombinant neurotoxins Hk2a, Hk7a, Hk8a, Hk16a, originally from a sea anemone species Anthopleura sp., were obtained by fusion expression of their genes in Escherichia coli. These neurotoxins were composed of 47 amino acid residues, among which the differences were found at positions 14, 22, 25, and 37, respectively. The effects of the four neurotoxins on single‐channel current of sodium in rat hippocampal neurons were studied by cell‐attached patch clamp. Each neurotoxin 2 μM could modulate the sodium channel by prolonging the opening dwell time and increasing the open probability, but did not change the amplitude of sodium channel currents. Based on the studies of the structure–function relationship, we found that Hk7a displayed the biggest increase of the open probability because His14 (from Arg14) makes its structure seem more compact in comparison with the other three toxins and Ap‐A. Phe25 (Hk8a, Hk16a), which varied from Ala25 (Hk2a, Hk7a), showed that phenyl group might interfere with other key amino acid residue to decrease the activity of toxins. Arg37 (from His37) in Hk8a contributed to decrease of open probability. In our work, it was shown that these important amino acid sites might provide a reliable proof for the future pharmaceutical design. © 2009 Wiley Periodicals, Inc. J Biochem Mol Toxicol 23:244–255, 2009; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20285