bluuebunny/crossref_metadata_2025_split huggingface dataset:
Indigo production by Pseudomonas sp. J26, a marine naphthalene‐degrading strain
Authors: Mercadal Juan Pablo Riva , Isaac Paula , Siñeriz Faustino , Ferrero Marcela Alejandra 
Published: May 2010

AbstractA technique developed to determine naphthalene dioxygenase (NDO) activity was optimized and used to study the biotransformation of indole to indigo by Pseudomonas sp. J26 whole cells. The maximum production of indigo was achieved at 25 °C using 2.5 mM indole when J26 was grown in the complex medium JPP, while indole concentrations higher than 4 mM proved toxic for cells. The maximum rate of indigo production was 0.56 nmol min–1 mg dry biomass–1, obtaining 75.5 μM of indigo after 8 h of incubation, while a maximal concentration (138.1 μM) of indigo was obtained after 20 h. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Purification and characterization of novel extracellular cholesterol esterase from Acinetobacter sp.
Authors: Du Liangjun , Huo Ying , Ge Fanglan , YU Jiajun , Li Wei , Cheng Guiying , Yong Bin , Zeng Lihuang , Huang Min 
Published: May 2010

AbstractCHE4‐1, a bacterial strain that belongs to the genus Acinetobacter and expresses high level of inducible extracellular cholesterol esterase (CHE), was isolated from feces of carnivore Panthera pardus var. The cholesterol esterase of the strain CHE4‐1 was purified by ultrafiltration followed with DEAE‐Sepharose FF chromatography and Phenyl‐Sepharose CL‐4B chromatography, and then by Sephadex G‐50 gel filtration. Different from other known microbial cholesterol esterase, the purified CHE from CHE4‐1 strain is a monomer with molecular weight of 6.5 kD and has high activity to both long‐chain and short‐chain cholesterol ester. Enzymatic activity was enhanced in the presence of metal ion Ca2+, Zn2+ and boracic acid, and was not significantly affected by several detergents including sodium cholate, Triton X100 and Tween‐80. The enzyme was found to be stable during long‐term aqueous storage at 4 °C, indicating its potential as a clinical diagnostic reagent. To the best of our knowledge, this is the first report regarding purification and characterization of CHE from Acinetobacter sp. The results demonstrated that this particular CHE is a novel cholesterol esterase. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Diversity of nitrogenase (nifH) genes pool in soybean field soil after continuous and rotational cropping
Authors: Xiao C. H. , Tang H. , Pu L. J. , Sun D. M. , Ma J. Z. , Yu M. , Duan R. S. 
Published: May 2010

AbstractDiazotrophs diversity in soybean is a topic requiring thorough investigation since the previous researches have focused on only rice, forest, grass, water, etc. In this research, iron‐only nitrogenase nifH gene was as genetic marker. PCR‐RFLP was used to investigate the difference of diazotrophs community diversity in the soil from the continuous cropping (CC) (the 5‐yr tilling of soybean) and the rotational cropping (RC) (soybean‐corn) soils in the northeast of China. A total of 36 isolates were genetically characterized. Most of the isolates closely related to Azospirillum and Azotobacter. Eighty‐six unique nifH gene sequences were obtained by cloning of the respective PCR products in two soil samples. It was found that the diversity of nifH genes in CC changed obviously compared with RC. Phylogenetic analysis indicated that most of the clones clustered together in a high homogeneity with some sequence retrieved from environmental representatives. The sequence diversity of nifH genes was high and the members of the Alphaproteobacteria were predominant in both samples. The experimental study also revealed the two non‐proteobacterial diazotrophs, firmicutes and euryarchaeota. Through this study, it can be assumed that different tillage perhaps affected the nifH gene‐containing population diversity. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Cell division in magnetotactic bacteria splits magnetosome chain in half
Authors: Staniland Sarah S. , Moisescu Cristina , Benning Liane G. 
Published: May 2010

AbstractCell division in magnetotactic bacteria has attracted much interest, speculation and hypothesis with respect to the biomineralised chains of magnetic iron‐oxide particles known as magnetosomes. Here we report direct Transmission Electron Microscopy (TEM) evidence that division occurs at a central point of the cell and the chain, cleaving the magnetosome chain in two. Additionally, the new magnetosome chain relocates rapidly to the centre of the daughter cell and the number of magnetosomes is directly proportional to the cell length, even during the division part of the cell cycle. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Candida species extracellular alcohols: production and effect in sessile cells
Authors: Martins Margarida , Henriques Mariana , Azeredo Joana , Rocha Sílvia M. , Coimbra Manuel A. , Oliveira Rosário 
Published: May 2010

AbstractCell‐cell signaling alcohol molecules were recently identified in Candida albicans and Candida dubliniensis supernatants. To date, it is not known whether these molecules are produced by other Candida species and their role in biofilm formation is not fully clarified. Herein, Candida parapsilosis and Candida tropicalis extracellular alcohols production by planktonic cultures was analyzed by headspace‐solid‐phase microextraction and gas chromatography‐mass spectrometry. Both these Candida species extracellular media contained E,E ‐farnesol, 1‐dodecanol, 2‐phenylethanol, and isoamyl alcohol but not E ‐nerolidol, as produced by C. albicans and C. dubliniensis. Moreover, the ability of these compounds to regulate C. albicans, C. dubliniensis, C. parapsilosis, and C. tropicalis sessile cells was assessed by adding the alcohols after 3 h of adhesion and 48 h of biofilm formation. After 24 h, biofilms were analyzed in terms of cellular mitochondrial activity and total biomass. E,E ‐Farnesol affected C. albicans and C. dubliniensis. E ‐Nerolidol and 1‐dodecanol elicited C. parapsilosis and C. tropicalis changes in further biofilm development. C. tropicalis was affected by 2‐phenylethanol and isoamyl alcohol triggered changes in C. albicans, C. dubliniensis, and C. tropicalis sessile cells. The results demonstrated that almost all of these alcohols are produced by these Candida species and also evidence the complexity of biofilm formation. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Effect of carbon source on pyrimidine biosynthesis in Pseudomonas oryzihabitans
Authors: West Thomas P. 
Published: May 2010

AbstractThe effect of carbon source on the regulation of pyrimidine biosynthesis in the opportunistic human pathogen Pseudomonas oryzihabitans was studied at the level of enzyme synthesis. Although pyrimidine supplementation of glucose‐grown Ps. oryzihabitans cells produced a slight but statistically significant effect on the de novo pyrimidine biosynthetic pathway enzyme activities, catabolite repression of the enzyme activities by glucose appeared to be occurring. Pyrimidine limitation experiments undertaken using an orotidine 5'‐monophosphate decarboxylase mutant strain grown on glucose indicated that repression of enzyme synthesis by pyrimidines was occurring. Following pyrimidine limitation of the mutant strain cells, dihydroorotase and dihydroorotate dehydrogenase activities were found to about double while aspartate transcarbamoylase and orotate phosphoribosyltransferase activities were slightly elevated compared to their activities in the mutant strain cells grown on excess uracil. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Tacit knowledge and emotional intelligence: the ‘intangible’ values of SMEs
Authors: Piperopoulos Panagiotis 
Published: May 2010

AbstractIn the present economic downturn, with financial assets that may be heavily devalued, the real value of the firm might come in the form of strategies for unleashing the creative and innovative power of the human mind?—?i.e., the development of an emotionally intelligent workforce.
Internalizing and externalizing classes in posttraumatic stress disorder: A latent class analysis
Authors: Forbes David , Elhai Jon D. , Miller Mark W. , Creamer Mark 
Published: May 2010

AbstractUsing latent class analysis (LCA) the typology of personality profiles of veterans with posttraumatic stress disorder (PTSD) was examined based on internalizing/externalizing dimensions of psychopathology. Latent class analysis on Minnesota Multiphasic Personality Inventory‐2 (MMPI‐2) Personality Psychopathology‐5 (PSY‐5) scale data from 299 Australian combat veterans with PTSD supported the model, identifying an optimal 4‐class solution, with PTSD externalizing class defined by aggressiveness and disconstraint, high and moderate internalizing classes differentiated on the extent of elevations in introversion and negative emotionality and elevation of psychoticism in the high internalizing class and a simple PTSD class with normal range scores. The model was validated using external self‐report and psychiatric‐interview‐derived diagnoses. A second exploratory LCA using broader comorbidity indicators (MMPI‐2 Restructured Clinical scales) demonstrated some support for, although limitations in, using nonpersonality measures to identify these classes directly.
Diagnostic accuracy of the trauma screening questionnaire after the 2005 London bombings
Authors: Brewin Chris R. , Fuchkan Nika , Huntley Zoe , Scragg Peter 
Published: May 2010

AbstractThe diagnostic accuracy of screening instruments in the immediate aftermath of disaster is unknown. Following the 2005 London bombings a 2‐year outreach program identified and screened affected persons. In 331 persons the accuracy of the Trauma Screening Questionnaire (TSQ) was compared to a DSM‐IV (Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition; American Psychiatric Association, 1994) diagnosis of posttraumatic stress disorder. The sensitivity of the TSQ was comparable with previous studies, but specificity was substantially lower. Specificity was lowest immediately after the bombings and rose steadily over the next 18 months. Specificity was also lower in non‐White individuals, who had elevated scores on the TSQ. The data are consistent with previous research showing elevated distress in ethnic minorities following trauma, and suggest limitations on the performance of screening instruments.
Rifaximin for the treatment of recurrent Clostridium difficile infection after liver transplantation: A case series
Authors: Neff Guy , Zacharias Victoria , Kaiser Tiffany E. , Gaddis Amy , Kemmer Nyingi 
Published: May 2010

Abstract
Previous data have suggested that the nonsystemic antibiotic rifaximin may be effective for the treatment of Clostridium difficile infection (CDI). This single-center retrospective study evaluated the efficacy of rifaximin for the treatment of CDI refractory to standard treatments in patients who had received liver transplants. Among 205 patients who had received liver transplants between July 2001 and December 2007, 3 patients with a confirmed diagnosis of C. difficile experienced recurrent diarrhea even though they received standard therapy. Patient 1, a 56-year-old male, patient 2, a 62-year-old male, and patient 3, a 73-year-old female, developed CDIs 190, 318, and 2310 days after transplantation, respectively. All patients experienced symptom recurrences after oral metronidazole therapy (250 mg 3 times daily for either 14 or 28 days) and after oral vancomycin therapy (125 mg 4 times daily for 14 days). Long-term vancomycin treatment (ie, 28 days) was required for patients 1 and 2. Vancomycin was discontinued in patient 3 because of increased creatinine levels. Oral rifaximin (400 mg 3 times daily) was initiated immediately after discontinuation of vancomycin therapy. Within 36 to 48 hours of the initiation of rifaximin treatment, diarrheal symptoms were resolved in all patients. After completing a 28-day course of rifaximin, patient 1 remained symptom-free during 185 days of follow-up, and patient 2 remained symptom-free during 250 days of follow-up. Patient 3 reported no symptoms within 155 days after the completion of rifaximin treatment. These findings suggest that rifaximin may be effective for the treatment of recurrent CDI and may provide a therapeutic option for CDI in immunocompromised patients. Liver Transpl 16:960-963, 2010. © 2010 AASLD.
Multi‐method research on consumer–brand associations: Comparing free associations, storytelling, and collages
Authors: Koll Oliver , von Wallpach Sylvia , Kreuzer Maria 
Published: May 2010

AbstractWhat consumers know and think consciously and unconsciously about a brand influences their attitudes and behaviors toward the brand and ultimately brand success. Therefore, keeping track of what consumers know is advisable. This article considers the value of using three approaches to assess brand knowledge: free association technique, storytelling, and collage‐creation. Each method is suitable for tapping and reproducing different aspects of brand knowledge. The empirical study combines the three methods in an explorative setting to retrieve consumer brand knowledge regarding a major sports brand. The study compares knowledge that each method elicits and provides brand management with recommendations how to decide when to use each method and whether to employ one or more of these methods. © 2010 Wiley Periodicals, Inc.
Creating visual narrative art for decoding stories that consumers and brands tell
Authors: Megehee Carol M. , Woodside Arch G. 
Published: May 2010

AbstractCreating visual narrative art (VNA) of stories that consumers and brands tell achieves several objectives. First, creating VNA revises and deepens sense making of the meaning of events in the story and what the complete story implies about oneself and others. Second, creating VNA surfaces unconscious thinking of the protagonist and other actors in the story as well as the storyteller (recognizing that in many presentations of stories an actor in the story is also the story teller); unconscious thinking in stories relating to consumer and brand experiences reflect one or more archetype (Jung, 1916/1968) fulfillments by the protagonist and the storyteller; given that almost all authors agree on a distinction between processes that are unconscious, rapid, automatic, and high capacity (System 1 processing) and those that are conscious, slow, and deliberative (System 2 processing; see Evans, 2008), VNA enables and enriches processing, particularly relating to System 1 processing—enabling more emotional versus rational processing. Third, creating VNA of stories is inherently and uniquely fulfilling/pleasurable/healing for the artist; using visual media allows artists to express emotions of the protagonist and/or audience member, to vent anger, or to report bliss about events and outcomes that words alone cannot communicate; VNA provides a tangible, emotional, and holistic (gestalt) experience that is uniquely satisfying and does so in a form that many audience members enjoy over and over again. This article elaborates on the rationales for its central proposition, briefly reviews relevant literature on VNA, and illustrates one mode of VNA for the complementary stories told by a consumer and brand. © 2010 Wiley Periodicals, Inc.
Brand consumption and narrative of the self
Authors: Schembri Sharon , Merrilees Bill , Kristiansen Stine 
Published: May 2010

AbstractThis study investigates how consumers use brands to construct their self. Focusing on the consumer's experience of brands, the study interprets consumer narratives on how brand consumption contributes to the construction of the self. The findings demonstrate that consumers use brands in different ways: symbolic, iconic, and indexical. Apart from the symbolism of brands used to construct the self, consumers also use brands that resemble something in an iconic manner. Additionally, consumers use brands that have a factual connection to something in an indexical way to construct the self. Given these findings, this paper therefore contributes to both theory and practice. Theoretically, the findings support semiotic theory and the relationships between the object, the sign, and the interpretant. More practically, this work shows that recognition of the experiential meaning of brands informs marketers and brand managers on how to effectively market brands. © 2010 Wiley Periodicals, Inc.
Cognitive and functional decline in Huntington's disease: Dementia criteria revisited
Authors: Peavy Guerry M. , Jacobson Mark W. , Goldstein Jody L. , Hamilton Joanne M. , Kane Amy , Gamst Anthony C. , Lessig Stephanie L. , Lee J. C. , Corey‐Bloom Jody 
Published: May 2010

AbstractThe importance of designating criteria for diagnosing dementia lies in its implications for clinical treatment, research, caregiving, and decision‐making. Dementia diagnosis in Huntington's disease (HD) is often based on criteria developed for Alzheimer's disease requiring memory loss. However, it is likely that other cognitive deficits contribute to functional impairment in HD before memory declines. The goal is to identify cognitive deficits that contribute to functional impairment to support dementia criteria that reflect HD neuropathology. Eighty‐four HD mutation‐positive subjects completed neuropsychological tests and the Unified Huntington's Disease Rating Scale Functional Independence Scale (FIS). Functional impairment was defined as 80 or below on the FIS. Speed of processing, initiation, and attention measures accounted for 70.0% of the variance in FIS ratings (linear regression) and correctly classified 91.7% of subjects as functionally impaired or intact (logistic regression). Measures of memory, motor impairment except dysarthria, neuroleptic use, and depressed mood did not improve prediction. A definition of HD dementia that includes cognitive impairment in at least two areas of cognition but does not require a memory deficit, in the context of impaired functional abilities and a deteriorating course, more accurately reflects HD neuropathology and could lead to improved research methods and patient care. © 2010 Movement Disorder Society
Does the historical literature on encephalitis lethargica support a simple (direct) relationship with postencephalitic Parkinsonism?
Authors: Vilensky Joel A. , Gilman Sid , McCall Sherman 
Published: May 2010

AbstractThis article and the subsequent one suggest that the currently accepted view of a simplistic (direct) relationship between encephalitis lethargica (EL) and postencephalitic Parkinsonism (PEP) is based on a incomplete evaluation of the epidemic period literature. In this article we provide a detailed analysis of the literature from the period that demonstrates that Parkinsonism was not initially part of acute EL symptomatology, that PEP was not typically the prevailing type of chronic EL and that oculogyric crises were never part of acute EL symptomatology and not initially associated with PEP. The second paper uses these finding, and also examines the clinical justifications for concluding that all patients with PEP had prior acute episodes of EL, to reevaluate the presumed direct etiologic relationship between EL and PEP. © 2010 Movement Disorder Society
REM sleep behavior disorder is not linked to postural instability and gait dysfunction in Parkinson
Authors: Benninger David H. , Michel Jan , Waldvogel Daniel , Candia Victor , Poryazova Rositsa , van Hedel Hubertus J.A. , Bassetti Claudio L. 
Published: May 2010

AbstractTo evaluate a potential association of REM‐sleep behavior disorder (RBD) with gait and postural impairment in Parkinson's disease (PD). Gait difficulties and postural impairment are frequent in PD and are a major cause of disability. Animal studies indicate a key role of the pedunculopontine nucleus (PPN) in gait, postural control, and REM sleep, and also in the pathophysiology of RBD. In humans, such an association has not been investigated. Twenty‐six patients with mild‐to‐moderate PD (13 with polysomnography confirmed and 13 with excluded RBD), and 20 age‐matched healthy controls were prospectively investigated. Gait assessment on a treadmill, and static and dynamic posturography were performed. PD patients with RBD do not differ from those without RBD in gait and postural control. Greater severity of PD or prevalence of gait and postural disturbances in the presence of RBD were not found. RBD was not associated with any particular motor phenotype. We found no association of RBD with gait disturbances and postural impairment. Human gait and postural control and RBD appear to depend upon different neuronal circuits. © 2010 Movement Disorder Society
Multishot PROPELLER for high‐field preclinical MRI
Authors: Pandit Prachi , Qi Yi , Story Jennifer , King Kevin F. , Johnson G. Allan 
Published: May 2010

AbstractWith the development of numerous mouse models of cancer, there is a tremendous need for an appropriate imaging technique to study the disease evolution. High‐field T2‐weighted imaging using PROPELLER (Periodically Rotated Overlapping ParallEL Lines with Enhanced Reconstruction) MRI meets this need. The two‐shot PROPELLER technique presented here provides (a) high spatial resolution, (b) high contrast resolution, and (c) rapid and noninvasive imaging, which enables high‐throughput, longitudinal studies in free‐breathing mice. Unique data collection and reconstruction makes this method robust against motion artifacts. The two‐shot modification introduced here retains more high‐frequency information and provides higher signal‐to‐noise ratio than conventional single‐shot PROPELLER, making this sequence feasible at high fields, where signal loss is rapid. Results are shown in a liver metastases model to demonstrate the utility of this technique in one of the more challenging regions of the mouse, which is the abdomen. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.
Real‐time single‐heartbeat fast strain‐encoded imaging of right ventricular regional function: Normal versus chronic pulmonary hypertension
Authors: Shehata Monda L. , Basha Tamer A. , Tantawy Wahid H. , Lima Joao A. , Vogel‐Claussen Jens , Bluemke David A. , Hassoun Paul M. , Osman Nael F. 
Published: May 2010

AbstractPatients with pulmonary hypertension and suspected right ventricular (RV) dysfunction often have dyspnea at rest, making reliable assessment of RV function using traditional breath‐holding methods difficult to perform. Using single‐heartbeat fast strain encoding (Fast‐SENC) imaging, peak systolic RV circumferential and longitudinal strains were measured in 11 healthy volunteers and 11 pulmonary hypertension patients. Fast‐SENC RV longitudinal strain and circumferential strain measurements were compared to conventional SENC and MR tagging, respectively. Fast‐SENC circumferential and longitudinal RV shortening correlated closely with SENC measurements (r = 0.86, r = 0.90, P < 0.001 for all). Circumferential strain, by conventional tagging, showed moderate correlation with Fast‐SENC in pulmonary hypertension patients only (r = 0.5, P = 0.003). A nonuniform pattern of RV circumferential shortening was depicted in both groups. Peak systolic circumferential strain was significantly reduced at the basal RV in pulmonary hypertension patients (−18.06 ± 3.3 versus −21.9 ± 1.9, P < 0.01) compared to normal individuals, while peak systolic longitudinal strain was significantly reduced at all levels (P < 0.01 for all). Fast‐SENC is a feasible and reliable technique for rapid quantification of RV regional function in a single‐heartbeat acquisition. Information derived from Fast‐SENC allows characterization of RV regional function in normal individuals and in pulmonary hypertension patients. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.