bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of thermal processing on IgE reactivity to lentil and chickpea proteins
Authors: Cuadrado Carmen , Cabanillas Beatriz , Pedrosa Mercedes M. , Varela Alejandro , Guillamón Eva , Muzquiz Mercedes , Crespo Jesús F. , Rodriguez Julia , Burbano Carmen 
Published: October 2009

AbstractIn the last years, legume proteins are gaining importance as food ingredients because of their nutraceutical properties. However, legumes are also considered relevant in the development of food allergies through ingestion. Peanuts and soybeans are important food allergens in Western countries, while lentil and chickpea allergy are more relevant in the Mediterranean area. Information about the effects of thermal‐processing procedures at various temperatures and conditions is scarce; therefore, the effect of these procedures on legume allergenic properties is not defined so far. The SDS‐PAGE and IgE‐immunoblotting patterns of chickpeas and lentils were analyzed before and after boiling (up to 60 min) and autoclaving (1.2 and 2.6 atm, up to 30 min). The results indicated that some of these treatments reduce IgE binding to lentil and chickpea, the most important being harsh autoclaving. However, several extremely resistant immunoreactive proteins still remained in these legumes even after this extreme treatment.
Four selenoproteins, protein biosynthesis, and Wnt signalling are particularly sensitive to limited selenium intake in mouse colon
Authors: Kipp Anna , Banning Antje , van Schothorst Evert M. , Méplan Catherine , Schomburg Lutz , Evelo Chris , Coort Susan , Gaj Stan , Keijer Jaap , Hesketh John , Brigelius‐Flohé Regina 
Published: October 2009

AbstractSelenium is an essential micronutrient. Its recommended daily allowance is not attained by a significant proportion of the population in many countries and its intake has been suggested to affect colorectal carcinogenesis. Therefore, microarrays were used to determine how both selenoprotein and global gene expression patterns in the mouse colon were affected by marginal selenium deficiency comparable to variations in human dietary intakes. Two groups of 12 mice each were fed a selenium‐deficient (0.086 mg Se/kg) or a selenium‐adequate (0.15 mg Se/kg) diet. After 6 wk, plasma selenium level, liver, and colon glutathione peroxidase (GPx) activity in the deficient group was 12, 34, and 50%, respectively, of that of the adequate group. Differential gene expression was analysed with mouse 44K whole genome microarrays. Pathway analysis by GenMAPP identified the protein biosynthesis pathway as most significantly affected, followed by inflammation, Delta‐Notch and Wnt pathways. Selected gene expression changes were confirmed by quantitative real‐time PCR. GPx1 and the selenoproteins W, H, and M, responded significantly to selenium intake making them candidates as biomarkers for selenium status. Thus, feeding a marginal selenium‐deficient diet resulted in distinct changes in global gene expression in the mouse colon. Modulation of cancer‐related pathways may contribute to the higher susceptibility to colon carcinogenesis in low selenium status.
Variation in the human lipidome associated with coffee consumption as revealed by quantitative targeted metabolomics
Authors: Altmaier Elisabeth , Kastenmüller Gabi , Römisch‐Margl Werner , Thorand Barbara , Weinberger Klaus M. , Adamski Jerzy , Illig Thomas , Döring Angela , Suhre Karsten 
Published: October 2009

AbstractThe effect of coffee consumption on human health is still discussed controversially. Here, we report results from a metabolomics study of coffee consumption, where we measured 363 metabolites in blood serum of 284 male participants of the Cooperative Health Research in the Region of Augsburg study population, aged between 55 and 79 years. A statistical analysis of the association of metabolite concentrations and the number of cups of coffee consumed per day showed that coffee intake is positively associated with two classes of sphingomyelins, one containing a hydroxy‐group (SM(OH)) and the other having an additional carboxy‐group (SM(OH,COOH)). In contrast, long‐ and medium‐chain acylcarnitines were found to decrease with increasing coffee consumption. It is noteworthy that the concentration of total cholesterol also rises with an increased coffee intake in this study group. The association observed here between these hydroxylated and carboxylated sphingolipid species and coffee intake may be induced by changes in the cholesterol levels. Alternatively, these molecules may act as scavengers of oxidative species, which decrease with higher coffee intake. In summary, we demonstrate strong positive associations between coffee consumption and two classes of sphingomyelins and a negative association between coffee consumption and long‐ and medium‐chain acylcarnitines.
Optimizing assignment of Tomahawk cruise missile missions to firing units
Authors: Newman Alexandra M. , Rosenthal Richard E. , Salmerón Javier , Brown Gerald G. , Price Wilson , Rowe Anton , Fennemore Charles F. , Taft Robert L. 
Published: October 2009

AbstractThe Tomahawk land‐attack cruise missile can be launched from a ship or submarine, and can deliver its warhead precisely to a target at long range. There are several variants of the Tomahawk missile, each with specialized capabilities. For each Tomahawk Missile Sequence Number (MSN) task (i.e., mission), the Tomahawk missile variants can be ranked with respect to their ability and cost effectiveness to perform that task. A given land attack strike order may involve a large number of Tomahawk missiles and numerous Tomahawk launch platforms. Operational planners select, in real time, feasible launch platforms to execute Tomahawk taskings. The Tomahawk tasking in a strike order includes not only primary assignments but also backup assignments. On board each launch platform, the precise allocation of specific Tomahawk missiles to the Tomahawk MSN task assigned is optimized with a model described here. We help naval operational planners select, in real time, appropriate feasible launch platforms to fulfill the Tomahawk tasking in a strike order. © 2009 Wiley Periodicals, Inc. Naval Research Logistics 58: 281–294, 2011
On online bin packing with LIB constraints
Authors: Epstein Leah 
Published: October 2009

AbstractIn many applications of packing, the location of small items below large items, inside the packed boxes, is forbidden. We consider a variant of the classic online one‐dimensional bin packing, in which items allocated to each bin are packed there in the order of arrival, satisfying the condition above. This variant is called online bin packing problem with LIB (larger item in the bottom) constraints. We give an improved analysis of First Fit showing that its competitive ratio is at most
$ {5 \over 2} = 2.5$, and design a lower bound of 2 on the competitive ratio of any online algorithm. In addition, we study the competitive ratio of First Fit as a function of an upper bound $ {1 \over d} $
(where d is a positive integer) on the item sizes. Our upper bound on the competitive ratio of First Fit tends to 2 as d grows, whereas the lower bound of two holds for any value of d. Finally, we consider several natural and well known algorithms, namely, Best Fit, Worst Fit, Almost Worst Fit, and Harmonic, and show that none of them has a finite competitive ratio for the problem. © 2009 Wiley Periodicals, Inc. Naval Research Logistics, 2009
Methoxy poly(ethylene glycol)‐b‐poly(octadecanoic anhydride)‐b‐methoxy poly(ethylene glycol) amphiphilic triblock copolymer nanoparticles as delivery vehicles for paclitaxel
Authors: Xing Jinfeng , Deng Liandong , Xie Chaopeng , Xiao Li , Zhai Yinglei , Jin Fengmin , Li Yimei , Dong Anjie 
Published: October 2009

AbstractA series of amphiphilic triblock copolymers, methoxy poly(ethylene glycol)‐b‐poly(octadecanoic anhydride)‐b‐methoxy poly(ethylene glycol) (mPEG‐b‐POA‐b‐mPEG), were prepared via melt polycondensation of methoxy poly(ethylene glycol) (mPEG) and poly(octadecanoic anhydride) (POA). mPEG‐b‐POA‐b‐mPEG were characterized by FTIR, 1H‐NMR, GPC, DSC, and XRD. Drug‐loaded mPEG‐b‐POA‐b‐mPEG nanoparticles (NPs) with spherical morphology and narrow size polydispersity index were prepared by nanoprecipitation technique with paclitaxel as the model drug. In vitro release behaviors of drug‐loaded NPs present that the biphasic process and the release mechanism of each phase are zero order drug releases. According to this study, mPEG‐b‐POA‐b‐mPEG NPs could serve as suitable delivery agents for paclitaxel and other hydrophobic drugs. Copyright © 2009 John Wiley & Sons, Ltd.
Simultaneous extraction of nucleic acids and proteins from tissue specimens by ultracentrifugation: A protocol using the high‐salt protein fraction for quantitative proteome analysis
Authors: Grzendowski Michael , Riemenschneider Markus J. , Hawranke Eva , Stefanski Anja , Meyer Helmut E. , Reifenberger Guido , Stühler Kai 
Published: October 2009

AbstractComprehensive molecular profiling of human tumor tissue specimens at the DNA, mRNA and protein level is often obstructed by a limited amount of available material. Homogenization of frozen tissue samples in guanidine isothiocyanate followed by ultracentrifugation over cesium chloride allows the simultaneous extraction of high‐molecular weight DNA and RNA. Here, we present a protocol for quantitative proteome analysis using the high‐salt protein fraction obtained as supernatant after ultracentrifugation for nucleic acid extraction. We applied this method to extracts from primary human brain tumors and demonstrate its successful application for protein expression profiling in these tumors using 2‐D DIGE, MS and Western blotting.
Proteomic analysis of Arabidopsis halleri shoots in response to the heavy metals cadmium and zinc and rhizosphere microorganisms
Authors: Farinati Silvia , DalCorso Giovanni , Bona Elisa , Corbella Michela , Lampis Silvia , Cecconi Daniela , Polati Rita , Berta Graziella , Vallini Giovanni , Furini Antonella 
Published: October 2009

AbstractArabidopsis halleri has the rare ability to colonize heavy metal‐polluted sites and is an emerging model for research on adaptation and metal hyperaccumulation. The aim of this study was to analyze the effect of plant–microbe interaction on the accumulation of cadmium (Cd) and zinc (Zn) in shoots of an ecotype of A. halleri grown in heavy metal‐contaminated soil and to compare the shoot proteome of plants grown solely in the presence of Cd and Zn or in the presence of these two metals and the autochthonous soil rhizosphere‐derived microorganisms. The results of this analysis emphasized the role of plant–microbe interaction in shoot metal accumulation. Differences in protein expression pattern, identified by a proteomic approach involving 2‐DE and MS, indicated a general upregulation of photosynthesis‐related proteins in plants exposed to metals and to metals plus microorganisms, suggesting that metal accumulation in shoots is an energy‐demanding process. The analysis also showed that proteins involved in plant defense mechanisms were downregulated indicating that heavy metals accumulation in leaves supplies a protection system and highlights a cross‐talk between heavy metal signaling and defense signaling.
Proteomic analysis of the secretome of human umbilical vein endothelial cells using a combination of free‐flow electrophoresis and nanoflow LC‐MS/MS
Authors: Tunica David Guillen , Yin Xiaoke , Sidibe Anissa , Stegemann Christin , Nissum Mikkel , Zeng Lingfang , Brunet Merce , Mayr Manuel 
Published: October 2009

AbstractHuman umbilical vein endothelial cells are the most widely used in vitro model for endothelial cells. Their secreted proteins, however, have not been comprehensively analysed so far. In this study, we accomplished to map the secretome of human umbilical vein endothelial cells by combining free‐flow electrophoresis with nanoflow LC‐MS/MS. This comprehensive analysis provides a basis for future comparative studies of protein secretion by endothelial cells in response to cardiovascular risk factors and is available on our website http://www.vascular‐proteomics.com.
Can ultrasonic energy efficiently speed 18O‐labeling of proteins?
Authors: Carreira Ricardo J. , Lodeiro Carlos , Diniz Mario S. , Moura Isabel , Capelo José L. 
Published: October 2009

AbstractWe report in this work on the robustness of ultrasonic energy as a tool to speed the isotopic labeling of proteins using the 18O‐decoupling procedure. The first part of the decoupling procedure, comprising protein denaturation, reduction, alkylation and digestion, is done in 8 min under the effects of an ultrasonic field whilst the second part, the isotopic labeling, was assayed with and without the use of ultrasonic energy. Our results clearly demonstrate that the 18O‐isotopic labeling in a decoupling procedure cannot be accelerated using an ultrasonic field.
ExoCarta: A compendium of exosomal proteins and RNA
Authors: Mathivanan Suresh , Simpson Richard J. 
Published: October 2009

AbstractExosomes, membrane microvesicles (40–100 nm) secreted by most cell types, can be isolated in several ways while characterizing them is heavily based on electron microscopy and, most importantly, the identification of exosome marker proteins. Researchers rely on the identification of certain exosomal marker proteins including Alix, CD9 and CD63 to confirm the presence of exosomes in their preparations. An evolutionary‐conserved set of protein molecules have been identified in most exosomes studied to date. However, with the complexity of tissue/cell type‐specific proteins being incorporated in the exosomes, some of these so‐called exosomal markers are not always present in all the exosomes. The presence of tissue/cell type‐specific proteins in exosomes allows researchers to isolate them using immunoaffinity capture methods. A compendium for exosomal proteomes will aid researchers in identifying proteins that were more commonly found in various exosomes (exosome markers) and those that are specific to certain tissue/cell type‐derived exosomes. Here, we describe ExoCarta, a compendium for proteins and RNA molecules identified in exosomes. ExoCarta is first of its kind and the resource is freely available to the scientific community through the web (http://exocarta.ludwig.edu.au). We believe that this community resource will be of great biological importance for any future exosome analyses.
Hydrogels: From soft contact lenses and implants to self‐assembled nanomaterials
Authors: Kopecek Jindrich 
Published: October 2009

AbstractHydrogels were the first biomaterials designed for clinical use. Their discovery and applications as soft contact lenses and implants are presented. This early hydrogel research served as a foundation for the expansion of biomedical polymers research into new directions: design of stimuli sensitive hydrogels that abruptly change their properties upon application of an external stimulus (pH, temperature, solvent, electrical field, biorecognition) and hydrogels as carriers for the delivery of drugs, peptides, and proteins. Finally, pathways to self‐assembly of block and graft copolymers into hydrogels of precise 3D structures are introduced. © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 5929–5946, 2009
Star polymers with POSS via azide–alkyne click reaction
Authors: Gungor Eda , Bilir Cigdem , Durmaz Hakan , Hizal Gurkan , Tunca Umit 
Published: October 2009

AbstractAzidopropyl‐heptaisobutyl‐substituted polyhedral oligomeric silsesquioxane (POSS‐N3) was reacted with 1,1,1‐tris[4‐(2‐propynyloxy)phenyl]‐ethane (1) and poly(ethylene glycol) (PEG)‐b‐poly(methyl methacrylate) (PMMA) copolymer with alkyne at its center (PEG‐PMMA‐alkyne) affording the first time synthesis of 3‐arm star POSS and PEG‐PMMA‐POSS 3‐miktoarm star terpolymer, respectively, in the presence of CuBr/N,N,N′,N″,N″‐pentamethyldiethylenetriamine as catalyst and N,N‐dimethylformamide/tetrahydrofuran as solvent at room temperature. The precursors and the target star polymers were characterized comprehensively by 1H NMR, GPC, and DSC. © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 5947–5953, 2009
Poly(ethylene‐co‐1,4‐cyclohexylenedimethylene terephthalate) copolyesters obtained by ring opening polymerization
Authors: González‐Vidal Nathalie , Martínez De Ilarduya Antxon , Muñoz‐Guerra Sebastián 
Published: October 2009

AbstractCyclic oligomer fractions of ethylene terephthalate c(ET)n and 1,4‐cyclohexylenedimethylene terephthalate c(CT)n were obtained by cyclodepolymerization of their respective polyesters, the former containing around 80 mol % of trimer and the latter with around 70 mol % of trimer to pentamer. Mixtures of these fractions at selected compositions were subjected to ring opening copolymerization to give a series of poly(ethylene‐co‐cyclohexylenedimethylene terephthalate) copolyesters with ET/CT comonomer ratios ranging from 90/10 to 10/90. The copolyesters were characterized by GPC and NMR, and their thermal properties were evaluated by DSC and TGA. They had essentially the same composition as the feed from which they were produced and had an average‐weight molecular weights between 30,000 and 40,000 g/mol with polydispersities between 2 and 2.7. The distribution of the monomeric units in these copolyesters was essentially at random although it evolved to be a blocky microstructure as the contents in the two comonomers became more dissimilar. Their thermal behavior was the expected one for these types of copolyesters with crystallinity and heating stability decreasing with the content in CT units. © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 5954–5966, 2009
Supramolecular assembly of H‐bonded side‐chain polymers containing conjugated pyridyl H‐acceptor pendants and various low‐band‐gap H‐donor dyes bearing cyanoacrylic acid groups for organic solar cell applications
Authors: Liang Tzung‐Chi , Chiang I‐Hung , Yang Po‐Jen , Kekuda Dhananjay , CHU Chih‐Wei , Lin Hong‐Cheu 
Published: October 2009

AbstractNovel supramolecular side‐chain polymers were constructed by complexation of proton acceptor (H‐acceptor) polymers, i.e., side‐chain conjugated polymers P1–P2 containing pyridyl pendants, with low‐band‐gap proton donor (H‐donor) dyes S1–S4 (bearing terminal cyanoacrylic acids) in a proper molar ratio. Besides unique mesomorphic properties confirmed by DSC and XRD results, the H‐bonds of supramolecular side‐chain structures formed by pyridyl H‐acceptors and cyanoacrylic acid H‐donors were also confirmed by FTIR measurements. H‐donor dyes S1–S4 in solid films exhibited broad absorption peaks located in the range of 471–490 nm with optical band‐gaps of 1.99–2.14 eV. Furthermore, H‐bonded polymer complexes P1/S1–P1/S4 and P2/S1–P2/S4 exhibited broad absorption peaks in the range of 440–462 nm with optical band‐gaps of 2.11–2.25 eV. Under 100 mW/cm2 of AM 1.5 white‐light illumination, the bulk heterojunction polymer solar cell (PSC) devices containing an active layer of H‐bonded polymer complexes P1/S1–P1/S4 and P2/S1–P2/S4 (as electron donors) mixed with [6,6]‐phenyl C61 butyric acid methyl ester (i.e., PCBM, as an electron acceptor) in the weight ratio of 1:1 were investigated. The PSC device containing H‐bonded polymer complex P1/S3 mixed with PCBM (1:1 w/w) gave the best preliminary result with an overall power conversion efficiency (PCE) of 0.50%, a short‐circuit current of 3.17 mA/cm2, an open‐circuit voltage of 0.47 V, and a fill factor of 34%. © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 5998–6013, 2009
Synthesis, characterization, and properties of polyurethanes containing 1,4:3,6‐dianhydro‐D‐sorbitol
Authors: Lee Chi‐Han , Takagi Hideki , Okamoto Hirotaka , Kato Makoto , Usuki Arimitsu 
Published: October 2009

AbstractTwo‐ and three‐component polyurethanes containing 1,4:3,6‐dianhydro‐D‐sorbitol (isosorbide) derived from glucose were synthesized using n‐BuSn(O)OH·H2O as a catalyst, and the thermal properties (Tg, Td) of the polymers were investigated by differential scanning calorimetry and thermogravimetric analysis. We carried out molds for polyurethanes, the molds of polyurethanes were obtained. The dynamic mechanical analyzes showed that the storage modulus values of the three‐component polymers were constant to a higher temperature than those of the two‐component polymers. The storage moduli (E′), loss moduli (E″), and values of tan δ for the polymers were obtained. The rigidity of three‐component polymers was increased by the introduction of bisphenol A and diphenylmethane group to two‐component polymer. © 2009 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 47: 6025–6031, 2009
Effect of PPO‐g‐MA on structures and properties of PPO/PA6/short glass fiber composites
Authors: Li Bo , Zhang Yong , Bai Xin , Wang Shuai , Ji Jiliang 
Published: October 2009

AbstractPoly(2,6‐dimethyl‐1,4‐phenylene oxide)/polyamide 6 (PPO/PA6) blends were reactively compatibilized by maleic anhydride (MA) grafted PPO (PPO‐g‐MA) and reinforced by short glass fibers (SGF) via melt extrusion. An observation of the SGF‐polymer interface by scanning electronic microscope (SEM) together with etching techniques indicated that the PPO‐g‐MA played a decisive role in the adhesion of polymers to SGF. The rheological behavior was investigated by capillary rheometer, and the addition of PPO‐g‐MA, and SGF could increase the viscosity of the PPO/PA6 blends. The analysis of fiber orientation and distribution in the PPO/PA6/SGF composites showed PPO‐g‐MA favored to the random dispersion of SGF. The statistic analysis of SGF length showed that PPO‐g‐MA was helpful to maintain the fiber length during melt‐processing. For the composites at a given SGF content of 30 wt %, the addition of PPO‐g‐MA increased the tensile strength from 59.4 MPa to 97.1 MPa and increased SGF efficiency factor from 0.028 to 0.132. The experimental data were consistent with the theoretical predictions of the extension of Kelly‐Tyson model for tensile strength. The fracture toughness of the composites was investigated by single edge notch three‐point bending test. © 2009 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 47: 2188–2197, 2009
Isothermal crystallization behavior of exfoliated‐PP/IMMT nanocomposites via in situ polymerization
Authors: Liu Xin , He Aihua , Du Kai , Han Charles C. 
Published: October 2009

AbstractHighly exfoliated isotactic‐polypropylene/alkyl‐imidazolium modified montmorillonite (PP/IMMT) nanocomposites have been prepared via in situ intercalative polymerization. TEM and XRD results indicated that the obtained composites were highly exfoliated PP/IMMT nanocomposites and the average thickness of IMMT in PP matrix was less than 10 nm, and the distance between adjacent IMMT particles was in the range of 20–200 nm. The isothermal crystallization kinetics of highly exfoliated PP/IMMT nanocomposites were investigated by using differential scanning calorimeter(DSC) and polarized optical microscope (POM). The crystallization half‐time t1/2, crystallization peak time tmax, and the Avrami crystallization rate constant Kn showed that the nanosilicate layers accelerate the overall crystallization rate greatly due to the nucleation effect, and the crystallization rate was increased with the increase in MMT content. Meanwhile, the crystallinity of PP in nanocomposites decreased with the increase in clay content which indicated the PP chains were confined by the nanosilicate layers during the crystallization process. Although the well‐dispersed silicate layers did not have much influence on spherulites growth rate, the nucleation rate and the nuclei density increased significantly. Accordingly, the spherulite size decreased with the increase in MMT content. © 2009 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 47: 2215–2225, 2009