bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transcriptional activity of TAp63 promoter is regulated by c‐jun
Authors: Yao Jeng‐Yuan , Pao Chia‐Chu , Chen Jan‐Kan 
Published: July 2010

AbstractThe transcription factor p63 belongs to the p53 protein family and plays an important role in epithelial development. Recent studies showed that p63 is over‐expressed in some human squamous cell carcinomas of the head and neck, suggesting a role in carcinogenesis. The p63 gene contains two promoters and alternative promoter usage generates two groups of proteins with (TAp63) or without (ΔNp63) the transactivation domain. Although the roles of TAp63 in epithelial development have been described in numerous recent studies, the regulation of its expression has not been elucidated. In this study, we showed that the transcriptional activity of the TAp63 promoter and TAp63 protein level were both up‐regulated by an increased c‐jun activity in Hep3B human hepatocellular carcinoma cell. Moreover, the elevated TAp63 expression was coincided with an increased binding of c‐jun to the TAp63 promoter. Point mutation of the sp1 binding site within the TAp63 promoter region attenuated the effect of c‐jun on TAp63 expression. Knockdown of TAp63 expression by shRNA led to increased proliferation of Hep3B cell compared to that of the mock cell, suggesting a growth suppressive effect of TAp63. J. Cell. Physiol. 225: 898–904, 2010. © 2010 Wiley‐Liss, Inc.
Ectopia cordis in a fetus with mosaic trisomy 16
Authors: Arnaoutoglou Christos , Meditskou Soultana , Keivanidou Anastasia , Manthou Marilena , Anesidis Nikolaos , Assimakopoulos Efstratios , Athanasiadis Apostolos , Kumar Sailesh 
Published: July 2010

AbstractEctopia cordis and mosaic trisomy 16 are two rare fetal anomalies, which have not been reported in association. We report a case of an isolated ectopia cordis at 11+3 weeks. Subsequent embryological examination confirmed thoracic ectopia cordis with normal heart structure and array comparative genomic hybridization of fetal tissue detected trisomy 16 mosaicism. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound 38:386‐388, 2010
Seasonal changes of myostatin expression and its relation to body Mass acclimation in the Djungarian hamster, Phodopus sungorus
Authors: Braulke L.J. , Heldmaier G. , Berriel Diaz M. , Rozman J. , Exner C. 
Published: July 2010

AbstractThe Djungarian hamster is an animal that is prominent for distinct seasonal adaptations. Cued by shortening day length in autumn they spontaneously exhibit reductions in food intake, body mass (BM), fat mass and also in lean mass (LM). The mechanisms behind the seasonal regulation of body composition are only partly resolved. Although most studies focused on the participation of body fat in seasonal body weight regulation, we addressed the influence of LM, moreover of muscle mass (MM) on seasonal BM changes. Therefore, we analyzed body composition, MM and the expression of myostatin, a hormone negatively regulating muscle growth and differentiation, in Djungarian hamsters in response to naturally changing photoperiod in winter compared to long photoperiod (LP). Winter‐acclimated hamsters upregulated myostatin mRNA when compared with hamsters adapted to natural and artificial LP, whereas MM remained unchanged when compared with natural LP. Moreover, in natural short photoperiod, individual myostatin expression levels were negatively correlated with MM. These results suggest that myostatin is under seasonal control in order to regulate MM and hence contributes to the overall LM and therefore BM changes in seasonal mammals. J. Exp. Zool. 313A:548–556, 2010. © 2010 Wiley‐Liss, Inc.
Characterization and phylogenetic analysis of vitellogenin coding sequences in the Antarctic fish Trematomus bernacchii
Authors: Barucca Marco , Biscotti Maria Assunta , Forconi Mariko , Regoli Francesco , Olmo Ettore , Canapa Adriana 
Published: July 2010

AbstractVitellogenin is the yolk protein precursor. Multiple vitellogenins identified in several teleosts have been attributed different roles in the control of egg buoyancy and in early embryonic vs. late larval nutrition. In this study, the cDNA encoding VtgAa was characterized in the Antarctic fish Trematomus bernacchii (suborder Notothenioidei). The sequence contains 4,964 nucleotides and encodes 1,629 amino acids of the precursor molecule. To gain insights into the evolution of vitellogenin in Antarctic fishes, we identified the partial sequence of vtgAb, and vtgAa and vtgAb partial sequences of five other notothenioids. The phylogenetic analysis highlighted a close correlation between the Vtg amino acid sequences of the six Antarctic species and VtgAa and VtgAb of other perciforms. Finally, analysis of the ratio of vtgAa to vtgAb expression, evaluated in T. bernacchii by real‐time PCR, showed a considerably greater expression of vtgAa in different periods of austral summer. J. Exp. Zool. (Mol. Dev. Evol.) 314B:645–652, 2010. © 2010 Wiley‐Liss, Inc.
Comparative punting kinematics and pelvic fin musculature of benthic batoids
Authors: Macesic Laura J. , Kajiura Stephen M. 
Published: July 2010

AbstractAlthough the majority of batoid elasmobranchs, skates and rays, are benthically associated, benthic locomotion has been largely overlooked in this group. Only skates have been previously described to perform a form of benthic locomotion termed “punting.” While keeping the rest of the body motionless, the skate's pelvic fins are planted into the substrate and then retracted caudally, which thrusts the body forward. In this study, we demonstrate that this form of locomotion is not confined to the skates, but is found across a range of phylogenetically and morphologically diverse batoid species. However, only the clearnose skate, Raja eglanteria, and the lesser electric ray, Narcine brasiliensis, performed “true punting,” in which only the pelvic fins were engaged. The yellow stingray, Urobatis jamaicensis, and the Atlantic stingray, Dasyatis sabina, performed “augmented punting,” in which pectoral fin movement was also used to generate thrust. Despite this supplemental use of pectoral fins, the augmented punters failed to exceed the punting capabilities of the true punters. The urobatid and the true punters all punted approximately half their disc length per punt, whereas the dasyatid punted a significantly shorter distance. The skate punted significantly faster than the other species. Examination of the pelvic fin musculature revealed more specialized muscles in the true punters than in the augmented punters. This concordance of musculature with punting ability provides predictive power regarding the punting kinematics of other elasmobranchs based upon gross muscular examinations. In contrast to previous assumptions, our results suggest that benthic locomotion is widespread among batoids. J. Morphol., 2010. © 2010 Wiley‐Liss, Inc.
Halving imaging time of whole brain diffusion spectrum imaging and diffusion tractography using simultaneous image refocusing in EPI
Authors: Reese Timothy G. , Benner Thomas , Wang Ruopeng , Feinberg David A. , Wedeen Van J. 
Published: July 2010

AbstractPurposeTo increase the efficiency of densely encoded diffusion imaging of the brain, such as diffusion spectrum imaging (DSI), we time‐multiplex multiple slices within the same readout using simultaneous image refocusing echo‐planar imaging (SIR‐EPI).Materials and MethodsInefficiency in total scan time results from the long time of diffusion encoding gradient pulses which must be repeated for each and every image. We present a highly efficient multiplexing method, simultaneous image refocusing (SIR), for reducing the total scan time of diffusion imaging by nearly one‐half. SIR DSI is performed in 10 minutes rather than 21 minutes, acceptable for routine clinical application.ResultsTwo identical studies were completed, comparing conventional single‐slice EPI DSI and SIR‐EPI DSI, showing equal signal‐to‐noise ratio (SNR) and contrast and small differences in registration, likely due to typical subject motion. Comparison of DSI and DTI tractographs showed matching quality and detection of white matter tracts.ConclusionThe net reduction to nearly half the number of diffusion encoding gradient pulses in SIR‐EPI significantly reduces acquisition times of DSI and DTI. J. Magn. Reson. Imaging 2009;29:517–522. © 2009 Wiley‐Liss, Inc.
Agglutinine in marinen Braunalgen
Authors: Wagner M. , Wagner Barbara 
Published: July 2010

AbstractExtracts from the brown algaeFucus vesiculosus, F. serratus, andHalidrys siliquosaagglutinated human erythrocytes of groups A, B, 0, and AB as well as erythrocytes from some animal species, ascites tumour cells from mice, and streptococci of many groups of theLANCEFIELDsystem. The extract ofChorda filumdid not show haemagglutination but agglutinated ascites tumour cells and streptococci of a few groups.
Eisenoxydierende, stäbchenförmige Bakterien III. Quantitative Untersuchungen zum Effekt von Eisen auf das Wachstum
Authors: Kullmann K.‐H. , Schweisfurth R. 
Published: July 2010

AbstractWachstum mit Oxalat und Citrat konnte bei 2 der von SCHULZE u. SCHWEINSFURTH (1971) isolierten Stämme durch Eisen gefördert werden, wobei ein Fe(II)‐Zusatz einen stärkeren Effekt zeigte als ein Zusatz von Fe(III). Bei Stamm 31 konnte der positive Effekt durch Eisen auf ein physiologisch günstiges tieferes pH zurückgeführt werden, das sich bei der Bildung von Fe(III)‐hydroxid einstellt. St. 68 dagegen zeigte auch bei konstantem pH einen positiven Wachstumseffekt von Eisen. Mikroaerophile Bedingungen im Kulturmedium mit Eisen hemmten das Wachstum.
Regulative influence ofo‐aminobenzoic acid on the biosynthesis of nourseothricin in cultures ofStreptomyces nourseiJA 3890b III. Change of redox state of nicotinamide‐adenine‐dinucleotides in the presence of aminobenzoic acids
Authors: Gräfe U. , Bocker H. , Reinhardt G. , Thrum H. 
Published: July 2010

Abstracto‐Aminobenzoic acid (OABA, anthranilic acid) and related compounds which are known to stimulate the biosynthesis of streptothricin‐type antibiotic nourseothricin byStreptomyces nourseiJA 3890b were found to increase strongly the NADH/NAD+ ratio in growing mycelium of this strain suggesting that these effectors are capable of interfering with the function of the respiratory chain. In parallel, a complex shift of metabolism was induced shown by simultaneous alteration of mycelial activities of alanine dehydrogenase, glutamine synthetase, and glutamate dehydrogenase. These changes may be responsible for the observed delay of amino acid catabolism and may improve the precursor supply of the secondary metabolism.
Effects of some inorganic elements on nitrogen‐fixation in blue‐green algae and some ecological aspects of pollution
Authors: Henriksson L. E. , Dasilva E. J. 
Published: July 2010

AbstractNitrogen‐fixation by two species of Nostoc, one of them a lichen phycobiont, was generally stimulated by low concentrations of arsenic, cadmium, nickel, lead, palladium, and zinc. Higher concentrations (0.025 to 0.125 ppm) of arsenic, nickel, and palladium were also stimulatory; however, higher concentrations of cadmium, lead, and zinc tended to inhibit fixation. With the exception of palladium and zinc at low concentrations these six tested elements tended to inhibit nitrogen‐fixation in Chlorogloea fritschii and Westiellopsis sp.
Rhizosphere fungi of five plants in Egypt
Authors: El‐Hissy F. T. , Abdel‐Hafez S. I. , Abdel‐Kader M. I. 
Published: July 2010

Abstract75 species which belong to 25 genera were collected during this investigation. All of these fungi were previously isolated from Egyptian soils and other sources. The total count and the number of genera and species in the rhizosphere soil of the 5 plants were regularly higher than in non‐rhizosphere soil.The composition of the rhizosphere fungus flora was considerably influenced by the type and the age of the plant. The most prevalent fungi in the rhizosphere of the test plants after 45, 90, and 150 days were as follows: in Helianthus annuus, Stachybotrys atra and Aspergillus niger; in Chrysanthemum coronarium, Cladosporium herbarum, A. sydowii and Penicillium funiculosum; in Nigella sativa, Fusarium moniliforme and A. sydowii; in Datura innoxia, A. fumigatus and A. terreus; in Hyoscyamus muticus, C. herbarum and A. sydowii; and in soil, P. funiculosum, A. terreus and A. niger. The counts of total fungi and most of the common fungal species, except in the case of Datura innoxia, reached their maxima after 90 days and sharply declined after 150 days.
Gallertlager einer besonderen Mikroorganismengesellschaft an verbautem Grubenholz
Authors: Kraepelin G. , Passern D. 
Published: July 2010

AbstractConspicuous gelatinous deposits found on permanetly drenched pitwood planks in an old ore mine were analyzed microscopically. They contained a very characteristic microbial community consisting primarily of bacteria producing various kinds of slime capsules, stalks, hyphae or other appendages. The high degree of morphological diversity and the remarkable enrichment of differentiated cell types may reflelct selective advantages realized under the continous pressure imposed by such an unusual biotope.
Schutz zellfremder DNA vor wirtskontrollierter Restriktion in Bakterienzellen. II. Schutz des Plasmids pSF2124 durch die ocr+ Genfunktion der Bakteriophagen T3 und T7
Authors: Reuter Monika , Krüger D. H. , Scholz D. , Rosenthal H. A. 
Published: July 2010

AbstractWhen restriction‐active Escherichia coli cells (rm) are transformed with the pSF2124 plasmid, a common vector in experimental gene transfer, the efficiency of transformation (e.o.t.) is lowered by 2 orders of magnitude compared with restriction‐negative (rm or rm) recipient cells due to restriction of the pSF2124 DNA by endoR.EcoP1.Preinfection of rm cells with UV‐inactivated ocr+ phages (T3, T7, T3sam−) still able to express their early genes protects the plasmid DNA against restriction by endoR.EcoP1: The e.o.t. of rm recipient cells with pSF2124 attains the same high value as that of r−m− cells. The specific role of the ocr+ gene function was demonstrated by the use of ocr− mutants (T3/R7, T7/ D111): Preinfection with such phage mutants does not increase the e.o.t. of rm cells. An unspecific e.o.t. alteration of restriction‐negative (r−m−) recipient cells by ocr+ or ocr− phages was excluded.The ocr− gene function can be exploited to protect pSF2124 against DNA restriction. The recipient cells survive the process of phage preinfection and transformation and stably replicate themselves as well as the plasmid DNA.
Untersuchungen zur Frage einer Laugung von Erzen mit heterotrophen Mikroorganismen–Entwicklung eines Screening‐Verfahrens
Authors: Klages D. , Meyer I. , Schwartz W. , Näveke R. 
Published: July 2010

AbstractBesides leaching of sulfidic ores with thiobacilli, a second way of microbial leaching is tested with carbon‐heterotrophic fungi and bacteria for those types of ores, where thiobacilli fail to work. The active agents are metabolic products, f. i. organic acids, which are released into the medium producing water soluble compounds with heavy metalsA screening method is described for recognizing active strains in samples of soil, metal‐containing sites, mining heaps and other biocoenoses, using the dilution method in plates with different media. The critical signs of supposed leaching activity are halo formations around growing colonies in turbid agar media with suspended particles of Ca carbonate or Ca phosphates of low solubility, and in a second group of experiments with insoluble compounds of heavy metals, f. i. oxides. The leaching activity was tested with metal compounds suspended in liquid media and inoculated with active strains of the screening program. First results were reached with oxides of copper (CuO), uranium (U3O8) and an uranium‐containing phosphorite.
Leukaemomycin‐geblockte Mutanten des Streptomyces griseus und ihre Pigmente
Authors: Wagner Christina , Stengel Cornelia , Eritt Inge , Schumann G. , Fleck W. F. 
Published: July 2010

AbstractIn a continued search for leukaemomycin‐blocked mutants of three leukaemomycin‐producing strains IMET JA 3933, IMET JA 5142 and IMET JA 5570 of Streptomyces griseus, 32 mutants producing aerial mycelium and spores were detected. Furthermore, in all mutants cosynthetic capability has been observed. This report describes characterization of leukaemomycin‐blocked mutants obtained by mutagenic treatment experiments using NTG and combined UV‐/X‐rays. According to the biosynthetic capability for anthracyclinones or other pigments the mutants could be divided into six classes. The first class contains 14 leukaemomycin‐blocked mutants unable to synthesize anthracyclinones. Besides two classes of mutants (12)synthesizing well‐known anthracyclinones as epsilon‐rhodomycinone, 7‐deoxy‐epsilon‐rhodomycinone, 11‐deoxy‐derivatives of daunomycinone, three new classes of mutants (6) synthesizing reddish‐brown, brown and blue‐violet pigments on solid media with structures not elucidated as yet, will be described.
Rubredoxin‐Reductase in Rohextrakten von Acinetobacter calcoaceticus in Abhängigkeit von C‐Quelle und Wachstumsphase
Authors: Claus R. , Kleber H.‐P. 
Published: July 2010

AbstractBy immunization of rabbits with discelectrophoretically purified rubredoxin‐reductase from Ac. calcoaceticus an antiserum against this enzyme was prepared. The antiserum was monospecifical to a diaphoretic activity, which had a RF‐value identical with the rubredoxin‐reductase‐RF value.It has been shown by discelectrophoresis, immunoelectrophoresis, OUCHTERLONY technique and kinetic investigations that crude extracts of bacteria grown on C16, malate, succinate or acetate contain rubredoxin‐reductase.By the LAURELL technique we demonstrated that the amount of the enzyme is nearly independent of the carbon source. There are only differences during the different growth phases.
Isolation of membrane‐associated folded chromosomes from Anacystis nidulans
Authors: Ellinger A. , Dworsky P. , Weisshäupl V. 
Published: July 2010

AbstractParticles containing folded DNA were isolated from the blue‐green alga Anacystis nidulans. The structure of these particles is vesicle‐like and similar to that of membrane‐associated nuclear bodies which had been isolated form Escherichia coli under comparable conditions. The sedimentation constant is between 8000 and 9000 Svedbergs. The DNA is inside the particles and is attached to the thylakoid membranes.
Anthracyclic products fromStreptomyces erythromogenes nov. sp.Biotransformation of daunomycin (Dn) by an acellular preparation and synergism between Dn and some known antibiotics
Authors: Abdallah Nadia M. , Devys M. , Barbier M. 
Published: July 2010

AbstractThe filtrate broth as well as mycelium of the new strainStreptomyces erythrochromogenes nov. sp.isolated from Saudi Arabian soil, produce the antitumor antibiotic daunomycin1and two anthracyclic derivatives: 7‐deoxy 13‐dihydrodaunomycinone2and 7‐deoxy daunomycinone4. The biotransformation of1to2and4by an acellular enzyme preparation from the strain was found to be NADPH and/or NADH dependent.Mixtures of daunomycin1with chloramphenicol or penicillin showed superior antimicrobial activities againstBacillus subtilisICC strain, than the individual antibiotics.
Anwendung der Dielektrophorese für die präparative Trennung thermotoleranter Hefezellen
Authors: Krause G. , Schade W. , Glaser R. , Gräger B. 
Published: July 2010

AbstractDielectrophoresis as a new method for preparative separation of cells is being presented. This method responds to differences in the polarization of cells, which correlate with a number of complex physiological properties of cells. It is shown that yeast cells of different thermotolerant properties can be differentiated by dielectrophoresis. Moreover, the application of this method permitted to separate cells with a high selectivity, which produce more biomass at a temperature of 40°C than the initial population.Investigations on the ability of cells to survive in an inhomogeneous a. c. field have shown that the number of dead cells at frequencies of 105 cps and 106 cps and at field strengths (on the surface of the central electrode) below 2.4 × 105 V/m is small. This fact should be considered when picking out suitable separation conditions.
Lysogenie und lysogene Konversion bei methylotrophen Bakterien II. Lysogene Konversion bei fakultativ methanolassimilierenden Acetobacter‐Stämmen
Authors: Wünsche L. , Kiesel B. , Fischer H. 
Published: July 2010

AbstractThe lysogenic state of the methylotrophic strain Acetobacter MB 58/1 is completely demonstrated by curing and lysogenization experiments. During these investigations we found that some phenotypic characteristics are modified by the presence or loss of the prophage MO 1. It could be shown that changes of the serological behaviour, the adsorption of the phages and the sensitivity against oxytetracycline are caused by lysogenic conversion. The phenotypic alterations of the bacterial cells induced by the phage genome are the result of modifications of the lipopolysaccharide structures on the cell surface. In the case of oxytetracycline resistance, interactions between the modified lipopolysaccharide structures and specific transport proteins of the cell membranes must be assumed.
Modification by genetic changes of the pleiotropic interference of butyrolactone‐type autoregulators with differentiation of Streptomyces griseus
Authors: Gräfe U. , Eritt I. , Reinhardt G. , Krebs D. , Fleck W. F. 
Published: July 2010

AbstractTwo series of aerial‐mycelium‐negative (Amy−), anthracycline‐nonproducing (Ant−) mutants were obtained from ancestral Amy+Ant+ strains of S. griseus: a) derivatives represented by the met− strain 39 which could not differentiate although they were still producing both the butyrolactone‐type autoregulator 1 and NADP‐glycohydrolase, and b) mutants whose incapability to form spores and anthracycline pigments was apparently caused by the loss of autoregulator production. These latter mutants responded to the addition of 1 or the naturally occurring dihydro derivative 2 with complete or at least partial reconstitution of differentiation‐associated functions. All of the b)‐type mutant strains exhibited similar biochemical alterations in the presence of 1 or 2 regardless of the presence of additional genetic changes in the primary metabolism. Two mutants, however, displayed an altered pattern of secondary product formation. In submerged cultures the major biochemical changes observed in presence of 1 (or 2) were an increase of the lipid level in the mycelium, an alteration of the lipid composition, and a stimulation of neutral proteinase production. All of the blocked autoregulator‐negative mutants were discernible from the ancestral strains and strain 39 by their lack of NADP‐glycohydrolase production. This suggested the existance of a common genetic locus or a common pleiotropic regulator gene controling both gene functions. Present ideas concerning the role of butyrolactone‐type autoregulator 1 as a pleiotropic effector molecule interacting with development of S. griseus are summarized in a hypothetical scheme.
Genetic analysis of sexual hybrids and protoplast fusion products of the yeast Saccharomycopsis lipolytica
Authors: Kurischko Cornelia , Spata Liselotte 
Published: July 2010

AbstractProtoplast fusion of genetically marked Saccharomycopsis lipolytica strains was carried out succesfully. Mitotic segregation was induced to prove the presence of parental markers in fusion products. Methylbenzimidazol‐2‐yl‐carbamate has been shown to induce efficiently mitotic segregation in this yeast. The segregation pattern of genetic markers in a sexual hybrid was compared to that of a parasexual hybrid.