bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stock assessment and projections incorporating discard estimates in some years: an application to the hake stock in ICES Divisions VIIIc and IXa
Authors: Fernández C. , Cerviño S. , Pérez N. , Jardim E. 
Published: April 2010

Abstract
Fernández, C., Cerviño, S., Pérez, N., and Jardim, E. 2010. Stock assessment and projections incorporating discard estimates in some years: an application to the hake stock in ICES Divisions VIIIc and IXa. – ICES Journal of Marine Science, 67: 1185–1197. A Bayesian age-structured stock assessment model is developed to take into account available information on discards and to handle gaps in the time-series of discard estimates. The model incorporates mortality attributable to discarding, and appropriate assumptions about how this mortality may change over time are made. The result is a stock assessment that accounts for information on discards while, at the same time, producing a complete time-series of discard estimates. The method is applied to the hake stock in ICES Divisions VIIIc and IXa, for which the available data indicate that some 60% of the individuals caught are discarded. The stock is fished by Spain and Portugal, and for each country, there are discard estimates for recent years only. Moreover, the years for which Portuguese estimates are available are only a subset of those with Spanish estimates. Two runs of the model are performed; one assuming zero discards and another incorporating discards. When discards are incorporated, estimated recruitment and fishing mortality for young (discarded) ages increase, resulting in lower values of the biological reference points Fmax and F0.1 and, generally, more optimistic future stock trajectories under F-reduction scenarios.
Multilineage differentiation of dental follicle cells and the roles of Runx2 over‐expression in enhancing osteoblast/cementoblast‐related gene expression in dental follicle cells
Authors: Pan K. , Sun Q. , Zhang J. , Ge S. , Li S. , Zhao Y. , Yang P. 
Published: April 2010

AbstractObjectives:  Dental follicle cells (DFCs) provide the origin of periodontal tissues, and Runx2 is essential for bone formation and tooth development. In this study, pluripotency of DFCs was evaluated and effects of Runx2 on them were investigated.Materials and methods:  The DFCs were induced to differentiate towards osteoblasts, adipocytes or chondrocytes, and alizarin red staining, oil red O staining or alcian blue staining was performed to reveal the differentiated states. Bone marrow stromal cells (BMSCs) and primary mouse fibroblasts served as controls. DFCs were also infected with recombinant retroviruses encoding either full‐length Runx2 or mutant Runx2 without the VWRPY motif. Western blot analysis, real‐time real time RT‐PCR and in vitro mineralization assay were performed to evaluate the effects of full‐length Runx2 or mutant Runx2 on osteogenic/cementogenic differentiation of the cells.Results:  The above‐mentioned staining methods demonstrated that DFCs were successfully induced to differentiate towards osteoblasts, adipocytes or chondrocytes respectively, confirming the existence of pluripotent mesenchymal stem cells in dental follicle tissues. However, staining intensity in DFC cultures was weaker than in BMSC cultures. Real‐time PCR analysis indicated that mutant Runx2 induced a more pronounced increase in expression levels of OC, OPN, Col I and CP23 than full‐length Runx2. Mineralization assay also showed that mutant Runx2 increased mineralization nodule formation more prominently than full‐length Runx2.Conclusions:  Multipotent DFCs can be induced to differentiate towards osteoblasts, adipocytes or chondrocytes in vitro. Runx2 over‐expression up‐regulated expression levels of osteoblast/cementoblast‐related genes and in vitro enhanced osteogenic differentiation of DFCs. In addition, mutant Runx2‐induced changes in DFCs were more prominent than those induced by full‐length Runx2.
Cell proliferation and cell cycle alterations in oesophageal p53‐mutated cancer cells treated with cisplatin in combination with photodynamic therapy
Authors: Compagnin C. , Mognato M. , Celotti L. , Canti G. , Palumbo G. , Reddi E. 
Published: April 2010

AbstractObjectives:  The major goal of anti‐cancer therapies is selective destruction of tumour cells with minimum side effects on normal cells. Towards this aim, combination of different therapeutic modalities has been evaluated for improving control of neoplastic diseases and quality of life for the patient. Photodynamic therapy (PDT) is a procedure for treatment of various types of cancer, but its combination with other established treatments has not been evaluated in detail. We have used KYSE‐510 cells from a human oesophageal carcinoma as an in vitro model to investigate whether cisplatin (CDDP) could be combined with PDT to increase cell death with respect to single treatments.Materials and methods:  p53‐mutated KYSE‐510 cells were treated with CDDP alone or in combination with PDT. Analyses of cell viability, cell cycle progression and apoptosis induction were carried out at specific times after treatments.Results:  Decrease in cell viability, cell cycle arrest at the G2/M‐ and S‐phases boundary, and apoptosis induction were observed after single and combined treatments.Conclusions:  Our results show that low CDDP doses (0.25–1 μm) induce cell mortality and cell cycle perturbation, which were more evident when given in combination with PDT, but in contrast to work of other authors no synergistic activity was found. Apoptosis occurred via intrinsic pathways in treated cells, although it did not represent the predominant mode of cell death.
Isolation of therapeutically functional mouse bone marrow mesenchymal stem cells within 3 h by an effective single‐step plastic‐adherent method
Authors: Hsiao F. S.‐H. , Cheng C.‐C. , Peng S.‐Y. , Huang H.‐Y. , Lian W.‐S. , Jan M.‐L. , Fang Y.‐T. , Cheng E. C.‐H. , Lee K.‐H. , Cheng W. T.‐K. , Lin S.‐P. , Wu S.‐C. 
Published: April 2010

AbstractObjectives:  Isolation of mouse mesenchymal stem cells (mMSCs), by the approach of plastic adherence, has been difficult due to persistent contamination by haematopoietic cells (HCs); we have observed that this contamination was due to engagement between HCs and mMSCs. The HCs can be lifted together with the mMSCs despite their insensitivity to trypsin digestion. Herein, we provide a single‐step procedure to rapidly segregate mMSCs from HC contaminants using transient lower‐density plastic adherence (tLDA).Materials and methods:  The tLDA was performed by replating bone marrow adherent cells at lower density (1.25 × 104 cells/cm2) than usual, allowing for transient adherence of no more than 3 h, followed by trypsin digestion. tLDA‐isolated cells were evaluated by immunophenotyping, multi‐differentiation potentials, immunosuppressive properties, and therapeutic potential as demonstrated by symptoms of osteoporosis.Results:  The single‐step tLDA method can effectively eliminate the persistent HC contaminants; tLDA‐isolated cells were phenotypically equivalent to those reported as mMSCs. The isolated cells possessed classic tri‐lineage differentiation potential into osteogenic, adipogenic and chondrogenic lineages and had immunosuppressive properties. After intravenous transplantation, they migrated into the allogeneic bone marrow and rescued hosts from osteoporosis symptoms, demonstrating their therapeutic potential.Conclusions:  We have developed a simple and economical method that effectively isolates HC‐free, therapeutically functional mMSCs from bone marrow cell adherent cultures. These cells are suitable for various mechanistic and therapeutic studies in the mouse model.
Expansion of mouse sertoli cells on microcarriers
Authors: Shi B. , Zhang S. , Wang Y. , Zhuang Y. , Chu J. , Zhang S. , Shi X. , Bi J. , Guo M. 
Published: April 2010

AbstractBackground:  Sertoli cells (SCs) have been described as the ‘nurse cells’ of the testis whose primary function is to provide essential growth factors and create an appropriate environment for development of other cells [for example, germinal and nerve stem cells (NSCs), used here]. However, the greatest challenge at present is that it is difficult to obtain sufficient SCs of normal physiological function for cell transplantation and biological medicine, largely due to traditional static culture parameter difficult to be monitored and scaled up.Objective:  Operational stirred culture conditions for in vitro expansion and differentiation of SCs need to be optimized for large‐scale culture.Materials and methods:  In this study, the culturing process for primary SC expansion and maintaining lack of differentiation was optimized for the first time, by using microcarrier bead technology in spinner flask culture. Effects of various feeding/refreshing regimes, stirring speeds, seed inoculum levels of SCs, and concentrations of microcarrier used for expansion of mouse SCs were also explored. In addition, pH, osmotic pressure and metabolic variables including consumption rates of glucose, glutamine, amino acids, and formation rates of lactic acid and ammonia, were investigated in culture.Results:  After 6 days, maximal cell densities achieved were 4.6 × 106 cells/ml for Cytodex‐1 in DMEM/FBS compared to 4.8 × 105 cells/ml in static culture. Improved expansion was achieved using an inoculum of 1 × 105 cells/ml and microcarrier concentration of 3 mg/ml at stirring speed of 30 rpm. Results indicated that medium replacement (50% changed everyday) resulted in supply of nutrients and removal of waste products inhibiting cell growth, that lead to maintenance of cultures in steady state for several days. These conditions favoured preservation of SCs in the undifferentiated state and significantly increased their physiological activity and trophic function, which were assessed by co‐culturing with NSCs and immunostaining.Conclusion:  Data obtained in this study demonstrate the vast potential of this stirred culture system for efficient, reproducible and cost‐effective expansion of SCs in vitro. The system has advantages over static culture, which has major obstacles such as lower cell density, is time‐consuming and susceptible to contamination.
Offending behaviour in care: is children's residential care a ‘criminogenic’ environment?
Authors: Hayden Carol 
Published: April 2010

ABSTRACTThis paper focuses on offending behaviour and children in residential care. The paper considers whether children's residential care is a ‘criminogenic’ environment, i.e. whether this type of care environment helps to provide the conditions that produce crime or criminality. The paper draws on the findings from recently completed research on 10 children's homes in a large county local authority in England. This paper focuses on the patterns shown in trend data collected on problematic and offending data across these homes over a 7‐year period (2001–2007) and a 1‐year cohort study of 46 young people. Interviews with care staff and young people are used to contextualize these patterns. The data provide evidence of an environment where conflict and offending behaviour are common. It is argued that the residential care environment, particularly for older teenagers, often presents a set of risks that tend to reinforce offending behaviour and that this is in part due to its ‘last resort’ status.
Expression of CCR8 is increased in asthma
Authors: Mutalithas K. , Guillen C. , Raport C. , Kolbeck R. , Soler D. , Brightling C. E. , Pavord I. D. , Wardlaw A. J. 
Published: April 2010

SummaryBackground
Chemokines and their receptors could play key roles in the recruitment of T cells to the asthmatic lung. CCR8 is preferentially expressed on T‐helper type 2 cells, and is thought to play a role in the pathogenesis of human asthma.Objective
Determine the expression of CCR8 on T cells in blood, bronchoalveolar lavage (BAL) and bronchial mucosa from asthmatics and normal subjects.Methods
CCR8 expression in blood and BAL from asthma and normal subjects was studied using flow cytometry. CCR8 expression on IFN‐γ+ and IL‐4+/IL‐13+ blood and BAL T cells was studied following stimulation with Phorbol–Myristate–Acetate and Calcium Ionophore. Paraffin‐embedded bronchial biopsies were used to study CCR8 in bronchial epithelium.Results
The percentage of CD3+ cells expressing CCR8 in the blood was higher in asthmatics (4.7±0.4%) compared with normal subjects (3.0±0.4%; P<0.01). There was an approximately sixfold enrichment of CCR8 on IL‐4+/IL‐13+ cells compared with IFN‐γ+ T cells (P<0.001) in both asthmatic and normal subjects in both blood and BAL. Significantly more BAL T cells expressed CCR8 in asthmatic (8.6±0.8%) compared with normal subjects (3.9±0.7%) (P<0.01). In paired blood‐BAL samples from asthmatics, significantly more CCR8+CD3+ T cells were present in BAL (9.0±0.9%) than in blood (5.6±0.9%; P<0.05). There were more CCR8‐positive cells in bronchial biopsies from asthmatic (93±11 cells/mm2) compared with normal subjects (30±16 cells/mm2) (P<0.05). The ligand CCL1 was increased in the BAL of asthmatics compared with normal subjects (35±6 vs. 12.9±7 pg/mL; P<0.05).Conclusion
There may be a role for CCR8 in the recruitment of T cells to the lung in asthmatics. Cite this as: K. Mutalithas, C. Guillen, C. Raport, R. Kolbeck, D. Soler, C. E. Brightling, I. D. Pavord and A. J. Wardlaw, Clinical & Experimental Allergy, 2010 (40) 1175–1185.
Local administration of uridine suppresses the cardinal features of asthmatic airway inflammation
Authors: Müller T. , Grimm M. , De Vieira R. P. , Cicko S. , Dürk T. , Sorichter S. , Zissel G. , Idzko M. 
Published: April 2010

SummaryBackground
The immuno‐modulatory properties of nucleotides such as adenosine or inosine, have been described extensively. Recently, the nucleoside uridine and its analogue 4‐thiouridine have gained attention for their protective role in acute lung inflammation.Objective
In this study, we investigated the influence of uridine on asthmatic airway inflammation.Methods
We used the classical ovalbumin (OVA)–alum model, as well as a model of house dust mite‐(HDM)‐induced airway inflammation. The degree of inflammation was determined by bronchoalveolar lavage (BAL), histology, and measurement of bronchial hyperresponsiveness.Results
Intratracheal treatment of OVA‐sensitized animals with uridine before allergen challenge resulted in a reduction in total BAL cells and BAL eosinophils. This was accompanied by reduced tissue infiltration and diminished production of T helper type 2‐cytokines by mediastinal lymph node cells. Additionally, mice treated with uridine developed less bronchial hyperresponsiveness. Uridine was also effective in reducing airway inflammation in HDM‐induced asthma. The protective effects of uridine were independent of myeloid dendritic cell (mDC) function, because in vitro pre‐treatment of allergen‐pulsed DCs with uridine did not alter the degree of inflammation. However, uridine inhibited the release of pro‐inflammatory mediators in vivo and by cultured lung epithelial cells, suggesting an effect on lung structural cells.Conclusion
In summary, we were able to show that uridine inhibits the classical features of asthmatic airway inflammation. As uridine supplementation is well tolerated in humans, it might be a new therapeutic approach for the treatment of bronchial asthma. Cite this as: T. Müller, M. Grimm, R. P. de Vieira, S. Cicko, T. Dürk, S. Sorichter, G. Zissel and M. Idzko, Clinical & Experimental Allergy, 2010 (40) 1552–1560.
Low dose acetylsalicylic acid and shedding of microparticles in vivo in humans
Authors: Lubsczyk Barbara , Kollars Marietta , Hron Gregor , Kyrle Paul A , Weltermann Ansgar , Gartner Verena 
Published: April 2010

Eur J Clin Invest 2010; 40 (6): 477–482AbstractBackground  Microparticles (MPs) have procoagulant properties as shown in vitro and in animal models. Their role in haemostatic system activation at the site of a vascular injury in vivo in humans has not been studied.Material and methods  In a prospective randomized crossover study, 13 healthy volunteers were given 100 mg acetylsalicylic acid or placebo daily for 7 days. Number and cellular origin, expression of tissue factor (TF) and phosphatidylserine on MPs, and platelet and coagulation activation markers [β‐thromboglobulin (β‐TG), prothrombin fragment f1·2 (f1·2)] were measured in venous blood and in blood from a vascular injury (shed blood) by flow cytometry and immunoassays, respectively.Results  The number of MPs was significantly higher in shed blood than in venous blood. The majority of MPs were platelet derived. The expression of TF on MPs was low, but higher in shed blood than in venous blood. TF positive MPs were significantly higher in shed blood, which was due to an increase of MPs from platelets (PMPs). In shed blood, the number of TF expressing platelet‐derived MPs correlated with β‐TG, but not with f1·2. Low dose acetylsalicylic acid did not affect shedding of PMPs, neither in venous blood nor in shed blood.Conclusions  The release of PMPs locally at the site of platelet plug formation in humans indicates a possible role of MPs for haemostatic system activation in vivo. Low dose acetylsalicylic acid might not be strong enough to suppress shedding of PMPs in the microcirculation.
Transgenic overexpression of Abcb11 enhances biliary bile salt outputs, but does not affect cholesterol cholelithogenesis in mice
Authors: Wang Helen H. , Lammert Frank , Schmitz Anne , Wang David Q.‐H. 
Published: April 2010

Eur J Clin Invest 2010; 40 (6): 541–551AbstractBackground  Cholesterol gallstone disease is a complex genetic trait and induced by multiple but as yet unknown genes. A major Lith gene, Lith1 was first identified on chromosome 2 in gallstone‐susceptible C57L mice compared with resistant AKR mice. Abcb11, encoding the canalicular bile salt export pump in the hepatocyte, co‐localizes with the Lith1 QTL region and its hepatic expression is significantly higher in C57L mice than in AKR mice.Material and methods  To investigate whether Abcb11 influences cholesterol gallstone formation, we created an Abcb11 transgenic strain on the AKR genetic background and fed these mice with a lithogenic diet for 56 days.Result  We excluded functionally relevant polymorphisms of the Abcb11 gene and its promoter region between C57L and AKR mice. Overexpression of Abcb11 significantly promoted biliary bile salt secretion and increased circulating bile salt pool size and bile salt‐dependent bile flow rate. However, biliary cholesterol and phospholipid secretion, as well as gallbladder size and contractility were comparable in transgenic and wild‐type mice. At 56 days on the lithogenic diet, cholesterol saturation indexes of gallbladder biles and gallstone prevalence rates were essentially similar in these two groups of mice.Conclusion  Overexpression of Abcb11 augments biliary bile salt secretion, but does not affect cholelithogenesis in mice.
Quality of histopathological reporting on melanoma and influence of use of a synoptic template
Authors: Haydu Lauren E , Holt Phoebe E , Karim Rooshdiya Z , Madronio Christine M , Thompson John F , Armstrong Bruce K , Scolyer Richard A 
Published: April 2010

Haydu L E, Holt P E, Karim R Z, Madronio C M, Thompson J F, Armstrong B K & Scolyer R A (2010) Histopathology56, 768–774 Quality of histopathological reporting on melanoma and influence of use of a synoptic templateAims:  To evaluate the quality of histopathological reporting for melanoma in a whole population, to assess the influence on quality of the use of a synoptic template and thus to provide an evidence base to guide improvement in reporting melanoma pathology.Methods and results:  Histopathology reports of all primary invasive melanomas notified to the New South Wales Central Cancer Registry between October 2006 and October 2007 (n = 3784) were reviewed. A detailed audit of histopathology reports for consecutively diagnosed primary invasive melanoma over 6 months (n = 2082) was performed to assess the quality of each report based on compliance with the 2008 Clinical Practice Guidelines for the Management of Melanoma in Australia and New Zealand. Only half of the initial excision specimen reports included the essential components necessary to stage a melanoma patient according to the 2002 American Joint Committee on Cancer/International Union Against Cancer melanoma staging system. Report format was strongly correlated with completeness and validity of reporting: reports in a synoptic format, with or without a descriptive component, achieved the highest quality levels.Conclusions:  Even in a population with a high incidence of melanoma, concordance of pathology reports with current guidelines was comparatively low. Wider adoption of synoptic reporting is likely to increase report quality.
Identification of the nasal mucosa as a new target for leptin action
Authors: Taildeman Jasmien , Demetter Pieter , Rottiers Isabelle , Holtappels Gabriele , Bachert Claus , Cuvelier Claude A , Pérez‐Novo Claudina A 
Published: April 2010

Taildeman J, Demetter P, Rottiers I, Holtappels G, Bachert C, Cuvelier C A & Pérez‐Novo C A (2010) Histopathology 56, 789–798 Identification of the nasal mucosa as a new target for leptin actionAims:  Leptin is a key molecule in the regulation of energy homeostasis. Furthermore, several studies indicate that leptin also plays a pivotal role in immune and inflammatory responses. The aim of this study was to examine systemic and local nasal leptin and leptin receptor expression in patients with nasal polyposis and healthy controls.Methods and results:  Serum leptin and soluble leptin receptor levels were examined by enzyme‐linked immunosorbent assay (ELISA). The presence of leptin and leptin receptor mRNA was investigated using reverse transcriptase‐polymerase chain reaction (RT‐PCR), and tissue leptin and leptin receptor protein expression was analysed by immunohistochemistry and ELISA.Serum levels of biologically active leptin were significantly elevated in patients with nasal polyps compared with control subjects. These serum leptin levels were strongly correlated with the levels found in tissue in both study groups, although leptin was not significantly elevated in nasal polyp tissue. Using RT‐PCR, we showed that both leptin and its receptors were produced in nasal mucosa. Finally, immunohistochemistry showed that leptin and leptin receptor protein were expressed in several cells of the normal and inflamed nasal mucosa.Conclusions:  Leptin receptors and their biological ligand leptin are expressed in the nasal mucosa, suggesting a possible role in upper airway immunology.