bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dose of 3‐methylcholanthrene enhances vitamin C accumulation and mRNA expression of its transporter in the liver of ODS rats and in HepG2 cells
Authors: Sone Yasuko , Ueta Etsuko , Sannomaru Yasuko , Miyake Noriko , Sone Hirohito , Otsuka Yuzuru , Kondo Kazuo , Kurata Tadao , Suzuki Emiko 
Published: July 2011

AbstractPolycyclic aromatic hydrocarbon (PAH) compounds including 3‐methylcholanthrene induce harmful reactive intermediates and reactive oxygen species. This study reports the effect of 3‐methylcholanthrene on the accumulation of vitamin C and the expression of vitamin C transporters. ODS rats were given l‐ascorbic acid daily and intraperitoneal injections of 10 mg 3‐methylcholanthrene in total. On day 10, vitamin C concentrations and the expression of vitamin C transporter in the tissues were measured. As a result, the levels of sodium‐dependent vitamin C transporter (SVCTs) 1 and the l‐ascorbic acid concentration in 3‐methylcholanthrene‐treated livers and hepatocytes have increased significantly. However, the content of vitamin C in the urine and TBARS in the liver have not changed. These results suggest that the administration of 3‐methylcholanthrene elevates the requirement for vitamin C via (SVCTs) 1 due to xenobitics‐metabolizing, such as the induction of cytochrome P450 family. © 2011 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:369–376 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20403
A journal for our challenging, changing times: an editorial vision for the next five years of the Journal of Clinical Psychology
Authors: R. Elliott Timothy 
Published: July 2011

AbstractThe Journal of Clinical Psychology now features articles accepted by the new editorial team that will direct the journal over the next 5 years. Timothy R. Elliott serves as editor‐in‐chief and James Overholser is the senior associate editor. Associate editors are Linda Castillo, Kathleen Chwalisz, Stephanie Felgoise, and Bruce Rybarczyk. This editorial presents the editorial vision for the journal over the next 5 years, and presents changes in journal content. © 2011 Wiley Periodicals, Inc. J Clin Psychol 67:1–7, 2011.
Noninvasive detection of coronary artery wall thickening with age in healthy subjects using high resolution MRI with beat‐to‐beat respiratory motion correction
Authors: Scott Andrew D. , Keegan Jennifer , Mohiaddin Raad H. , Firmin David N. 
Published: July 2011

AbstractPurpose:To demonstrate coronary artery wall thickening with age in a small healthy cohort using a highly efficient, reliable, and reproducible high‐resolution MR technique.Materials and Methods:A 3D cross‐sectional MR vessel wall images (0.7 × 0.7 × 3 mm resolution) with retrospective beat‐to‐beat respiratory motion correction (B2B‐RMC) were obtained in the proximal right coronary artery of 21 healthy subjects (age, 22–62 years) with no known cardiovascular disease. Lumen and outer wall (lumen + vessel wall) areas were measured in one central slice from each subject and average wall thickness and wall area/outer wall area ratio (W/OW) calculated.Results:Imaging was successful in 18 (86%) subjects with average respiratory efficiency 99.3 ± 1.7%. Coronary vessel wall thickness and W/OW significantly correlate with subject age, increasing by 0.088 mm and 0.031 per decade respectively (R = 0.53, P = 0.024 and R = 0.48, P = 0.046). No relationship was found between lumen area and vessel wall thickness (P = NS), but outer wall area increased significantly with vessel wall thickness at 19 mm2 per mm (P = 0.046). This is consistent with outward vessel wall remodeling.Conclusion:Despite the small size of our healthy cohort, using high‐resolution MR imaging and B2B‐RMC, we have demonstrated increasing coronary vessel wall thickness and W/OW with age. The results obtained are consistent with outward vessel wall remodeling. J. Magn. Reson. Imaging 2011;. © 2011 Wiley‐Liss, Inc.
Determination of ricin by nano liquid chromatography/mass spectrometry after extraction using lactose‐immobilized monolithic silica spin column
Authors: Kanamori‐Kataoka Mieko , Kato Haruhito , Uzawa Hirotaka , Ohta Shigenori , Takei Yoshiyuki , Furuno Masahiro , Seto Yasuo 
Published: July 2011

AbstractRicin is a glycosylated proteinous toxin that is registered as toxic substance by Chemical Weapons convention. Current detection methods can result in false negatives and/or positives, and their criteria are not based on the identification of the protein amino acid sequences. In this study, lactose‐immobilized monolithic silica extraction followed by tryptic digestion and liquid chromatography/mass spectrometry (LC/MS) was developed as a method for rapid and accurate determination of ricin. Lactose, which was immobilized on monolithic silica, was used as a capture ligand for ricin extraction from the sample solution, and the silica was supported in a disk‐packed spin column. Recovery of ricin was more than 40%. After extraction, the extract was digested with trypsin and analyzed by LC/MS. The accurate masses of molecular ions and MS/MS spectra of the separated peptide peaks were measured by Fourier transform–MS and linear iontrap–MS, respectively. Six peptides, which were derived from the ricin A‐(m/z 537.8, 448.8 and 586.8) and B‐chains (m/z 701.3, 647.8 and 616.8), were chosen as marker peptides for the identification of ricin. Among these marker peptides, two peptides were ricin‐specific. This method was applied to the determination of ricin from crude samples. The monolithic silica extraction removed most contaminant peaks from the total ion chromatogram of the sample, and the six marker peptides were clearly detected by LC/MS. It takes about 5 h for detection and identification of more than 8 ng/ml of ricin through the whole handling, and this procedure will be able to deal with the terrorism using chemical weapon. Copyright © 2011 John Wiley & Sons, Ltd.
Maslinic acid, a natural inhibitor of glycogen phosphorylase, reduces cerebral ischemic injury in hyperglycemic rats by GLT‐1 up‐regulation
Authors: Guan Teng , Qian Yisong , Tang Xuzhen , Huang Menghao , Huang Longfei , Li Yunman , Sun Hongbin 
Published: July 2011

AbstractMaslinic acid (MA), a natural triterpene from Olea europaea L., is a well‐known inhibitor of glycogen phosphorylase and elicits multiple biological activities. The purpose of this study was to evaluate the effects of MA on focal cerebral ischemia in hyperglycemic rats. Adult rats were made hyperglycemic by intraperitoneal injection of streptozotocin and were given MA (50 mg/kg or 5 mg/kg) intragastrically for 14 consecutive days. Transient middle cerebral artery occlusion/reperfusion was then induced by a suture insertion technique. Results showed that diabetic rats pretreated with high‐dose MA had lower blood glucose levels, but both doses reduced infarct volumes and improved neurological scores. Less glutamate overflow was also observed in MA‐treated rats after 2 hr of ischemia followed by 24 hr and 72 hr reperfusion. In addition, MA treatment enhanced the glial glutamate transporter GLT‐1 expression at the protein and mRNA levels. However, the injection of dihydrokainate, a GLT‐1 glutamate transporter inhibitor, reversed the effect of MA. Previous studies have shown that suppression of glutamate uptake via nuclear factor‐κB (NF‐κB) activation is an important contributory factor in ischemia‐triggered glutamate excitotoxicity, and inhibition of NF‐κB could prevent ischemic suppression of glutamate uptake and GLT‐1 expression. In the present study, we showed that MA pretreatment attenuated ischemia‐induced translocation of NF‐κB p65 subunit to the nucleus. In conclusion, these findings demonstrate that, in addition to showing promising antidiabetic properties, MA has a direct beneficial effect in cerebral ischemic injury, which may be correlated with the promotion of glutamate clearance by NF‐κB‐mediated GLT‐1 up‐regulation. © 2011 Wiley‐Liss, Inc.
Identification and quantification of full‐length BK channel variants in the developing mouse cochlea
Authors: Sakai Yoshihisa , Harvey Margaret , Sokolowski Bernd 
Published: July 2011

AbstractMaxi‐K+ (BK) channel diversity is attributed to alternative splicing in the kcnma1 gene. The resultant variants manifest themselves in different cell types, tissues, and functions, such as excitation, metabolism, and signaling. Immunoelectron microscopy revealed immunogold particle labeling of BK in apical and basal regions of inner and outer hair cells, respectively. Additional labeling occurs in Deiters' cells and the inner mitochondrial membrane. Identification of full‐length sequences reveals 27 BK variants from embryonic and postnatal mouse inner ear, per classification by tail motif, VYR, DEC, and ERL, and by exon usage. Three predicted start codons are found encoding MAN, MSS, and MDA, of which MDA shows the greatest expression through all stages in development, whereas MAN is undetectable. Complex splice sites occur between exons 9 and 10 and between 21 and 23. Spliced‐in/out exons between 8 and 10 reveal a short fragment composed of exons 8 + 10, detectable on postnatal day (PD) 14 and PD30, and a longer fragment composed of exons 8 + 9 + 10 that is upregulated on embryonic day (ED) 14. Spliced‐in exons 22 or 23 are expressed on ED14 but decrease over time; however, exon 22 increases again on PD34. Using tail‐specific primers, qRT‐PCR from ED14, PD4, ‐14, and ‐30 shows that BK‐VYR and ‐ERL dominate expression on ED14, whereas DEC dominates after birth in all cochlear regions. The localization of BK and the changes in expression of its exons and tail types, by alternative splicing during development, may contribute to cochlear organization, acquisition of hearing, and intracellular signaling. © 2011 Wiley‐Liss, Inc.
Adamts5 (aggrecanase‐2) is widely expressed in the mouse musculoskeletal system and is induced in specific regions of knee joint explants by inflammatory cytokines
Authors: Wylie James D. , Ho Jason C. , Singh Shweta , McCulloch Daniel R. , Apte Suneel S. 
Published: July 2011

AbstractADAMTS5 (aggrecanase‐2) is an extracellular matrix‐degrading protease implicated in cartilage destruction in arthritis. Our goals were to determine expression sites of Adamts5 in the murine musculoskeletal system and in an ex vivo joint inflammation model. In mice with an intragenic LacZ reporter controlled by the Adamts5 promoter, β‐galactosidase staining was used to identify Adamts5 expressing cells. Mice expressing one wild‐type Adamts5 allele were used to determine distribution of Adamts5 mRNA, cleaved aggrecan and versican, and the ADAMTS5 activating enzymes furin and PACE4. Quantitative RT‐PCR and immunoblotting were used to validate the immunohistochemistry results. Adamts5 was expressed in mouse synovium, tenosynovium, bone marrow sinusoids, tendons, ligaments, ligament insertions, periosteal cells, and bone vasculature. In knee joint explants treated with IL‐1α and TNFα, Adamts5 expression was induced in tenocytes, synovium, and in patellar, but not femoral or tibial articular cartilage. In contrast, increased proteoglycan breakdown in tibial and femoral articular cartilage was associated with increased immunohistochemical staining of PACE4 and furin. These studies identify diverse cell types in the musculoskeletal system that express Adamts5. They also suggest that Adamts5 induction in joint components other than cartilage, and its post‐translational activation by PACE4 and/or furin may be important in the pathophysiology of arthritis. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:226–233, 2012
Salt effects on Origanum majorana fatty acid and essential oil composition
Authors: Baâtour Olfa , Kaddour Rym , Mahmoudi Hela , Tarchoun Imen , Bettaieb Iness , Nasri Nawel , Mrah Sabah , Hamdaoui Ghaith , Lachaâl Mokhtar , Marzouk Brahim 
Published: July 2011

AbstractBACKGROUND: The effects of salt on the essential oil yield and fatty acid composition of aerial parts of two marjoram varieties were investigated. Plants with 6 leaves were treated with NaCl (75mM).RESULTS: Salt treatment led to a reduction in aerial part growth. Salinity increased the fatty acid content more significantly in Tunisian variety (TV) than in Canadian variety (CV). CV showed an increase in double‐bond index (DBI) and a decrease in malondialdehyde content under salt stress, while the opposite was observed in TV. The DBI was mainly affected by a strong reduction in oleic and linoleic acids in TV, whereas a strong stimulation of linoleic acid in CV was observed. Salt decreased and increased the essential oil yield in TV and CV respectively. The main constituents of the essential oil of TV were trans‐hydrate sabinene and terpinen‐4‐ol, which showed a significant decrease under salt stress. In contrast, the main constituents of the essential oil of CV were sabinene and trans‐hydrate sabinene, which showed a significant decrease and increase respectively under salt stress.CONCLUSION: Marjoram oil is a rich source of many compounds such as essential oils and fatty acids, but the distribution of these compounds differed significantly between the two varieties studied. Copyright © 2011 Society of Chemical Industry
Sensory profiles of breast meat from broilers reared in an organic niche production system and conventional standard broilers
Authors: Horsted Klaus , Allesen‐Holm Bodil H , Hermansen John E , Kongsted Anne G 
Published: July 2011

AbstractBACKGROUND: Breast meat from broilers produced in very different production systems may vary considerable in sensory profile, which may affect consumer interests. In this study the aim was to evaluate differences in the sensory profiles of breast meat from five broiler products: two conventional standard products (A and B) and three organic niche genotypes (I657, L40 and K8) reared in an apple orchard.RESULTS: Thirteen out of 22 sensory attributes differed significantly between the products. The aroma attributes ‘chicken’, ‘bouillon’ and ‘fat’ scored highest and the ‘iron/liver’ aroma lowest for the niche products. The meat was more ‘tender’, ‘short’ and ‘crumbly’ and less ‘hard’ and ‘stringy’ in the standard products than in one or more of the niche products. Product ‘I 657’ was less ‘juicy’ than the rest. Products ‘I 657’ and ‘L 40’ were more ‘cohesive’ and tasted more ‘sourish’ and less of ‘sweet/maize’ than the standard products. The ‘overall liking’ score was significantly higher for the ‘K 8’ product than for the ‘Standard A’ and ‘L 40’ products. The ‘overall liking’ score was significantly correlated with the scores for aroma and taste of ‘chicken’, ‘umami/bouillon’, ‘iron/liver’ and ‘fat’ aroma.CONCLUSION: The sensory profiles differed particularly between conventional standard broilers and organic niche broilers, although differences were also found between breeds. The present study indicates that aroma and taste attributes were more important for the assessors than meat ‘tenderness’ for the overall liking of broiler meat. Copyright © 2011 Society of Chemical Industry
Diversity of lactic acid bacteria in sian‐sianzih (fermented clams), a traditional fermented food in Taiwan
Authors: Chen Yi‐sheng , Wu Hui‐chung , Li Ya‐han , Leong Kun‐hon , Pua Xiao‐hui , Weng Ming‐kai , Yanagida Fujitoshi 
Published: July 2011

AbstractBACKGROUND: Sian‐sianzih (fermented clams) is a popular traditional fermented food in Taiwan. The lactic acid bacteria (LAB) microflora in sian‐sianzih have not been studied in detail. In this study, LAB from sian‐sianzih were isolated, characterized and identified.RESULTS: A total of 186 cultures of LAB were isolated from seven sian‐sianzih samples and 29 cultures were isolated from its main raw substrate: clams. The identification results revealed up to 11 distinct bacterial species belonging to five genera in sian‐sianzih, and three species belonging to two genera in clams. The most common bacterial genera in sian‐sianzih were Lactobacillus and Weissella, followed by Leuconostoc, Pediococcus and Lactococcus. A regional similarity in LAB, with differences in diversity, was observed in the current study. On the other hand, Lactococcus lactis subsp. lactis was the most common species found in raw clam samples. The results also suggested that greater LAB diversity could be observed in wild clams than in cultured ones. Furthermore, antibacterial activities of the isolates were determined, and one Weisella hellenica strain showed inhibitory activity against the indicator strain Lactobacilluas sakei JCM 1157T. A sensory assessment of seven sian‐sianzih samples was also performed and the results indicated that diversity of LAB has a great effect on its aroma and taste formation.CONCLUSION: The results demonstrate that various LAB species are distributed in sian‐sianzih and have a great effect on the flavor of sian‐sianzih. Copyright © 2011 Society of Chemical Industry
Monolithic poly(butyl methacrylate–ethylene dimethacrylate–methacrylic acid) column for capillary electrochromatography
Authors: Ohyama Kaname , Horiguchi Daisuke , Kishikawa Naoya , Kuroda Naotaka 
Published: July 2011

AbstractA butyl methacrylate–ethylene dimethacrylate–methacrylic acid (MAA) monolithic column was prepared for capillary electrochromatography. The effect of MAA weight percentage on the EOF mobility and column efficiency was studied. BMA–EDMA–MAA monolith with higher content of MAA provided higher EOF mobility as well as higher efficiency. The effect of mobile‐phase composition and buffer pH was also investigated. The monolithic columns exhibited a good repeatability and reproducibility of column preparation with relative standard deviation values below 16% in the studied chromatographic parameters.
Separation and sweeping of metal ions with EDTA in CZE‐ESI‐MS
Authors: Quirino Joselito P. , Haddad Paul R. 
Published: July 2011

AbstractEthylenediaminetetraacetic acid (EDTA) in the background electrolyte (BGE) of capillary zone electrophoresis coupled to an electrospray ionization mass spectrometer is presented as an approach for the determination of metal ions. Significant signals for the metal–ligand complexes were observed even when EDTA was continually eluted from the capillary during the entire electrophoretic run. The signal‐to‐noise ratio was improved by the addition of ammonia to the sheath liquid and by using an acquisition m/z range above the m/z of EDTA. The LODs for the test metal ions (i.e. calcium(II), manganese(II), and zinc(II)) with conventional injection were around 1–2 mg/L with corrected peak areas that are linear from 8 to 100 mg/L. The presence of EDTA in the BGE was critical not only for the separation but also for sweeping via complexation as an on‐line sample concentration technique. The peak height of the test metal ions was improved at least tenfold with sweeping via EDTA complexation and yielded LODs in the μg/L range.
Use of tube radial distribution of ternary mixed carrier solvents for introduction of absorption reagent for metal ion separation and online detection into capillary
Authors: Fujinaga Satoshi , Jinno Naoya , Hashimoto Masahiko , Tsukagoshi Kazuhiko 
Published: July 2011

AbstractWhen ternary mixed solvents consisting of water‐hydrophilic/hydrophobic organic solvents are fed into a micro‐space under laminar flow conditions, the solvent molecules are radially distributed in the micro‐space. The specific fluidic behavior of the solvents is called the “tube radial distribution phenomenon (TRDP)”. A novel capillary chromatography method was developed based on the TRDP that creates the inner major and outer minor phases in a tube, where the outer phase acts as a pseudo‐stationary phase. This is called “tube radial distribution chromatography (TRDC)”. In this study, Chrome Azurol S as an absorption reagent was introduced into the TRDC system for metal ion separation and online detection. The fused‐silica capillary tube (75 μm id and 110 cm length) and water–acetonitrile–ethyl acetate mixture (3:8:4 volume ratio) including 20 mM Chrome Azurol S as a carrier solution were used. Metal ions, i.e. Co(II), Cu(II), Ni(II), Al(III), and Fe(III), as models were injected into the present TRDC system. Characteristic individual absorption characteristics and elution times were obtained as the result of complex formation between the metal ions and Chrome Azurol S in the water–acetonitrile–ethyl acetate mixture solution. The elution times of the metal ions were examined based on their absorption behavior; Co(II), Ni(II), Al(III), Fe(III), and Cu(II) were eluted in this order over the elution times of 4.7–6.8 min. The elution orders were determined from the molar ratios of metal ion to Chrome Azurol S and Irving–Williams series for bivalent metal ions.
Size separation and size determination of liposomes
Authors: Sakai‐Kato Kumiko , Ota Shigenori , Hyodo Kenji , Ishihara Hiroshi , Kikuchi Hiroshi , Kawanishi Toru 
Published: July 2011

AbstractWe developed a method for separating liposomes by size and determining their average diameters. Liposomes with different average diameters were separated on a monolithic silica capillary column, and the size of the liposomes corresponding to each peak was determined online with a dynamic light scattering detector coupled to the capillary liquid chromatography system. The calculated diameters for the separated liposomes were similar to the diameter values measured in batch mode. We demonstrate that this combination of a monolithic capillary column and light scattering detection could be used for size separation of liposomes and could provide more details about average diameters than batch‐mode analysis.
Simultaneous concentration enrichment and electrophoretic separation of weak acids on a microchip, using in situ photopolymerized carboxylate‐type polyacrylamide gels as the permselective preconcentrator
Authors: Yamamoto Sachio , Watanabe Yuki , Nishida Noriaki , Suzuki Shigeo 
Published: July 2011

AbstractA method for the simultaneous concentration and separation of weak acids using an acidic polyacrylamide gel, fabricated in the microfluidic channel of a commercial poly(methyl methacrylate)‐made microchip, is reported. This approach is based on simple photochemical copolymerization for the fabrication of a permselective preconcentrator. The intersection of the poly(methyl methacrylate)‐made microchip was filled with a gel solution comprising acrylamide, N,N′‐methylene‐bis‐acrylamide, and 2‐acrylamidoglycolic acid, with riboflavin as a photocatalytic initiator. In situ polymerization, near the cross of the sample outlet channel, was performed by irradiation with an argon ion laser beam that is also used as the light source for fluorimetric detection. The electrokinetic properties, combined with electrostatic repulsion between sample components and the anionic groups on the polyacrylamide gel, enable the entrapment and concentration of weak acids at the interface of the cathodic side of the gel plug. This method displays concentration factors of up to 105 within 3 min. The effectiveness of the ionic preconcentrator was demonstrated by the sensitive analysis of fluorescein isothiocyanate‐labeled amino acids.