bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel experimental model of MetALD in male mice recapitulates key features of severe alcohol-associated hepatitis
Authors: Babuta Mrigya , Morel Caroline , de Carvalho Ribeiro Marcelle , Datta Aditi Ashish , Calenda Charles , Copeland Christopher , Nasser Imad , Szabo Gyongyi 
Published: June 2024

Background:
The recent increase in the incidence of alcohol-associated hepatitis (AH) coincides with the obesity epidemic in the United States. However, current mouse models do not fully replicate the combined insults of obesity, metabolic dysfunction–associated steatohepatitis, and alcohol. The aim of this study was to develop a new mouse model that recapitulates the robust inflammatory and fibrotic phenotype characteristic of human MetALD.

Methods:
Eight- to 10-week-old male C57BL/6 mice were fed chow or high fat-cholesterol-sugar diet (metabolic dysfunction–associated steatohepatitis diet) and in each group, some received alcohol in drinking water (ad libitum) and weekly alcohol binges (EtOH) for 3 months. The liver was assessed for features of AH.

Results:
MetALD mice displayed increased liver damage indicated by highly elevated ALT and bilirubin levels compared to all other groups. Liver steatosis was significantly greater in the MetALD mice compared to all other experimental groups. The inflammatory phenotype of MetALD was also recapitulated, including increased IL-6 and IL-1β protein levels as well as increased CD68+ macrophages and Ly6G+ neutrophils in the liver. Sirius red staining and expression of collagen 1, alpha-smooth muscle actin indicated advanced fibrosis in the livers of MetALD mice. In addition, indicators of epithelial-to-mesenchymal transition markers were increased in MetALD mice compared to all other groups. Furthermore, we found increased ductular reaction, dysregulated hedgehog signaling, and decreased liver synthetic functions, consistent with severe AH.

Conclusions:
Alcohol administration in mice combined with metabolic dysfunction–associated steatohepatitis diet recapitulates key characteristics of human AH including liver damage, steatosis, robust systemic inflammation, and liver immune cell infiltration. This model results in advanced liver fibrosis, ductular reaction, decreased synthetic function, and hepatocyte dedifferentiation, suggesting a robust model of MetALD in mice.
Examining the Attitudes of Hypertension Patients Toward Using Complementary Treatments
Authors: Ilter Sümeyra Mihrap , Ovayolu Özlem 
Published: June 2024

The present study was conducted in a cross-sectional and descriptive design to examine the attitudes of hypertension patients toward using complementary treatments. The study was conducted with 173 patients who had the diagnosis of hypertension who applied to the Cardiology Clinics of a University Health Practice and Research Hospital between 15.12.2021 and 10.03.2022. Before starting the study, permission was obtained from the ethics committee, institution, and patients. The data of the study were collected through face-to-face interviews, a questionnaire, and the Attitude Scale Toward Using Complementary Treatments (ASUCT). The score to be obtained from the scale varies between 0 and 39 and a high score indicates a positive attitude toward complementary treatments. One-Sample Kolmogorov-Smirnov test was used to evaluate the homogeneity of the data. The Student t, Mann-Whitney U test, correlation, and ANOVA tests were also used to evaluate the data, and P < .05 was considered statistically significant. It was found that 55.0% of the patients were women, 31.2% were between the ages of 44 to 56, 63.0% had other chronic diseases, and 68.2% preferred herbal applications in the management of hypertension. It was also found that the mean ASUCT score of hypertensive individuals was 21.13 ± 2.99, and there was a statistically significant relationship between the mean ASUCT score of the patients and their gender, education level, and reasons for applying to herbal therapy (P < .05). Patients frequently preferred the consumption of pomegranate syrup (43.4%), garlic (36.4%), and quince leaf tea (30.8%) as non-drug applications in the management of hypertension. It was found that the attitudes of the hypertension patients toward using complementary treatments were at a “moderate” level and there was a relationship between their level of attitude toward using complementary treatments and gender, education, and the reasons for applying herbal therapy. In this respect, it is recommended to evaluate the attitudes of hypertension patients toward using complementary treatments, to learn the practices they prefer for disease management through patient-health care professional interaction, and to support patients for hypertension management.
Prognosticating Return-To-Play Time Following a Hamstring Strain Injury Using Early Flexibility Asymmetry and Musculoskeletal Ultrasound Imaging Outcomes: An Exploratory Study Among Canadian University Football Players
Authors: Gendron Patrick , Lamontagne Martin , Fournier-Farley Camille , Gagnon Dany H. 
Published: June 2024

Objective:
Identify key flexibility and point-of-care musculoskeletal ultrasound (POCUS) measures for prognosticating return-to-play (RTP) following a first hamstring strain injury (HSI) and informing the clinical decision–making process.

Design:
Exploratory prospective cohort study.

Setting:
Sport medicine and rehabilitation clinic of a Canadian university.

Participants:
One hundred and sixty-seven elite Canadian university football athletes followed over 5 seasons.

Interventions:
Clinical and POCUS measures collected within 7 days after HSI and preseason clinical measures.

Main Outcome Measures:
Active knee extension (AKE) and Straight Leg Raise (SLR) to quantify hamstring flexibility, POCUS-related outcomes to characterize tissue alteration, and RTP until full sport resumption were documented (categorized as Early [1-40 days] or Late [>40 days] RTP).

Results:
A total of 19 and 14 athletes were included in the Early RTP (mean RTP = 28.84 ± 8.62 days) and Late RTP groups (mean 51.93 ± 10.54 days), respectively, after having been diagnosed with a first HSI. For the clinical results, height and a greater flexibility asymmetry measure with the AKE or SLR when compared with both ipsilateral preseason and acute contralateral values significantly increases the chance of facing a long delay before returning to play (ie, RTP). For the POCUS-related results, the Peetrons severity score, extent of the longitudinal fibrillary alteration, and novel score lead to similar results.

Conclusions:
Early hamstring flexibility asymmetry following acute HSI, particularly the AKE, along with some POCUS-related measures are valuable in prognosticating late RTP following among Canadian university football athletes.
Safety and Efficacy of Cryotherapy for the Treatment of Renal Angiomyolipoma Related to Tuberous Sclerosis Complex: The Cryo-TSC Study
Authors: Kashiwagi Eiji , Hatano Takashi , Shimizu Kanichiro , Takamoto Atsushi , Tomita Koji , Osawa Takahiro , Sakuhara Yusuke , Hongo Fumiya , Hirota Tatsuya , Ushijima Yasuhiro , Asayama Yoshiki , Goto Shunsuke , Miki Jun , Matsukawa Akihiro , Kiyomi Fumiaki , Egawa Shin , Nasu Yasutomo , Shinohara Nobuo , Ukimura Osamu , Nakano Shuji , Arai Yasuaki , Eto Masatoshi 
Published: June 2024

Purpose:
Tuberous sclerosis complex (TSC) is a rare autosomal dominant inherited disease, in which approximately 60% to 80% of patients have bilateral angiomyolipoma (AML). There is a need for repeatable local treatments of TSC-AML. Cryotherapy is an option for renal tumors; however, its safety and efficacy for vascular tumors, such as TSC-AML, are unclear. The aim of this study was to establish the safety and efficacy of cryotherapy for TSC-AML.

Materials and Methods:
This was an open-label, single-arm, prospective phase II study to evaluate the safety and efficacy of cryotherapy for TSC-AML of ≤ 4 cm. Cryotherapy was performed using the CryoHit device with a percutaneous approach. Safety was assessed 1, 2, 6, and 9 months after cryotherapy, and efficacy was assessed 3 and 9 months after cryotherapy. The efficacy of cryotherapy was evaluated using the modified RECIST or RECIST criteria by independent central review. The primary end point was the disease control rate, and secondary end points were the overall response rate, safety, renal function, quality of life, and whether additional treatment was required.

Results:
The disease control rate was 100%, and the overall response rate was 93.3%. After 9 months of follow-up, the median estimated glomerular filtration rate decreased slightly (from 89.2 mL/min/1.73 m2 to 79.4 mL/min/1.73 m2). Quality of life was stable, and no additional treatment was needed. Three Grade 3 adverse events (hematuria, chronic kidney disease, and elevated aspartate aminotransferase) were observed in 3 patients, respectively, but no additional treatments were required.

Conclusions:
Cryotherapy for TSC-AML can be performed safely and efficiently. Cryotherapy can be a new therapeutic option for TSC-AML.

Trial Registration Number:
jRCTs072200039.
Therapeutic approaches for pulmonary hypertension in patients with chronic kidney disease
Authors: Tuttle Marcelle , Sarnak Mark J. , Navaneethan Sankar D. 
Published: June 2024

Purpose of review
Pulmonary hypertension is a common comorbidity in patients with chronic kidney disease (CKD), but therapeutic options are limited. We discuss the epidemiology of pulmonary hypertension in patients with CKD and review therapies for pulmonary hypertension with a focus on emerging treatments for pulmonary arterial hypertension (PAH).

Recent findings
The definition of pulmonary hypertension has been updated to a lower threshold of mean pulmonary artery pressures of more than 20 mmHg, potentially leading to more patients with CKD to qualify for the diagnosis of pulmonary hypertension. Endothelin receptor antagonists, a class of medications, which demonstrated efficacy in patients with PAH, have been shown to slow progression of CKD, but their efficacy in lowering pulmonary artery pressures and their effects on reducing cardiovascular mortality in this population remains unproven. Sotatercept, a novel activin signaling inhibitor, which was previously studied in dialysis patients has been shown to increase exercise capacity in patients with PAH. These studies may lead to new specific therapies for pulmonary hypertension in patients with CKD.

Summary
Pulmonary hypertension is common in patients with CKD. Although our understanding of factors leading to pulmonary hypertension in this population have evolved, evidence supporting disease-specific therapy in CKD is limited arguing for larger, long-term studies.
Sexual Behavior Among Emerging Adults in Africa: A Systematic Review and Meta-Analysis
Authors: Chea Stevenson K. , Kagonya Vincent A. , Abdullahi Osman , Abubakar Amina A. , Abbeddou Souheila , Michielsen Kristien , Hassan Amin S. 
Published: June 2024

Background
Estimates on sexual behavior (SB) among emerging adults (EmA) is varied in literature, which presents a challenge when designing targeted interventions. We aimed to summarize literature on prevalence and risk factors of SB among EmA in Africa.

Methods
A search for studies published in PubMed, Embase, and Psych Info by March 2023 was done. Studies involving EmA (18–25 years), conducted in Africa, and reporting 1 or more of 7 SBs were reviewed. Pooled prevalence estimates were summarized using forest plots. Heterogeneity in SB was explored. Risk factors were synthesized using a modified socioecological model.

Results
Overall, 143 studies were analyzed. Noncondom use had the highest pooled prevalence (47% [95% confidence interval {CI}, 42–51]), followed by study-defined SB (37% [95% CI, 25%–50%]) and concurrency (37% [95% CI, 21%–54%]), multiple sex partners (31% [95% CI, 25%–37%]), younger age at sexual debut (26% [95% CI, 20%–32%]), age-disparate relationships (24% [95% CI, 17%–32%]), and transactional sex (19% [95% CI, 13%–26%]). Heterogeneity was partially explained by sex, with female participants having higher pooled prevalence estimates compared with their male counterparts. In 4 of the 7 outcomes, alcohol/drug use was the most common risk factor.

Conclusions
Sexual behavior was common among EmA and differentially higher in emerging female adults. Noncondom use had the highest pooled prevalence, which may contribute to the transmission of HIV and other sexually transmitted infections. Interventions targeting emerging female adults and alcohol/drug use may reduce SB, which may in turn mitigate transmission of HIV and other sexually transmitted infections among EmA in Africa.
The effect of stretching exercises before orthotic treatment on the immediate in-orthosis correction of the patients with adolescent idiopathic scoliosis: A pilot study
Authors: Luo Chang Liang , Wu Hui Dong , Beygi Babak Hassan , Liu Shan , Zou Yi Ying , Shang Lin Jing , Wong Man Sang 
Published: June 2024

Background:
Stretching exercise is generally used for improving flexibility. However, its application to promote orthotic treatment for patients with adolescent idiopathic scoliosis (AIS) remains unknown.

Objective:
This study was to explore the effect of pre-orthosis stretching exercises on spinal flexibility and initial in-orthosis correction for the patients with AIS.

Study Design:
A pilot-controlled study.

Methods:
An experimental group (EG) of 13 subjects (10 girls and 3 boys) with AIS allocating to self-stretching exercises and a control group (CG) of 19 AIS subjects (14 girls and 5 boys) with no stretching before orthosis fitting were recruited. The spinal flexibility of the EG was evaluated with an ultrasound imaging system and physical measurements. The initial in-orthosis correction rates between the 2 groups were compared with the independent t test, and the correlation analysis between the spinal flexibility measured from ultrasound images and physical measurement was performed with the Pearson correlation test.

Results:
The initial Cobb angle of EG and CG were 25.70° ± 7.30° and 28.09° ± 5.58°, respectively. No significant difference was observed between the initial in-orthosis Cobb angle of EG (11.13° ± 6.80°) and CG (15.65° ± 9.10°) (p = 0.06). However, the spinal flexibility after stretching exercises was improved (p < 0.001), and the spinal flexibility changes measured with ultrasound and physical forward-bending method were significantly correlated (r = 0.57, p < 0.05).

Conclusion:
Stretching exercises before orthotic treatment could improve the spinal flexibility but did not cause a better in-orthosis correction. A study with a larger sample size and longer follow-up period should be conducted to investigate the long-term effect of stretching exercises.
Negative Pressure Wound Therapy for Chronic Wounds
Authors: Li Wenbo , Zheng Jianghong 
Published: June 2024

Abstract
Chronic wounds, including those caused by venous and arterial insufficiency, diabetic complications, and pressure-induced ulcers, pose significant treatment challenges. Negative pressure wound therapy has been increasingly used for managing these wounds. This treatment aims to promote wound healing, prepare the wound bed for further surgical intervention, minimize the risk of infection, and potentially shorten the time to wound healing. Considering variances in techniques applied in different regions globally, there is an emerging need to comprehensively evaluate the effectiveness of negative pressure wound therapy on chronic wounds. Unfortunately, detailed descriptions of the techniques applied to achieve negative pressure are often lacking in existing literature abstracts, posing challenges for direct comparisons. This review aims to analyze the application of negative pressure wound therapy in the treatment of chronic wounds, summarize its advantages and disadvantages, and further explore the potential value and future research direction of negative pressure wound therapy in the repair of chronic wounds.
Global and Latest Hotspots of Female Genital Plastic Surgery in the Past 20 Years
Authors: Li Tian-Hao , Li Zi-Ming , Yu Nan-Ze , Huang Jiu-Zuo , Long Xiao 
Published: June 2024

Background
An increasing number of surgical and nonsurgical interventions are available in the field of female genital plastic surgery. The rate of female genital plastic surgery has increased by nearly 220 percent over the past 5 years. Despite several studies on the topic, no relevant bibliometric analysis has been conducted.

Methods
We searched the Web of Science Core Collection for articles related to female genital plastic surgery. CiteSpace 6.1.R2 (Drexel University, USA) and VOSviewer 1.6.10.0 (Leiden University, the Netherlands) were used, and national distribution, institutions, journals, authors, and key words were analyzed and calculated.

Results
From 2003 to 2022, 1299 papers in the field of female genital plastic surgery were retrieved. There were more articles produced in the United States, and there were also two institutions in the Netherlands that were highly productive. A wide and close relationship has been established between researchers and institutions conducting female genital plastic surgery. Professor Bouman MB published the most articles on female genital plastic surgery in the Journal of Sexual Medicine. Female genital plastic surgery dominated the top 10 references with the highest local citation score. There were four clusters of key words with the most citations, and the most recently trending key words were “vaginal agenesis,” “transgender,” and “congenital adrenal hyperplasia.”

Conclusions
The purpose of this article is to provide a summary of the current research status focusing on female genital plastic surgery. It is hoped that more efforts will be made to promote the development of female genital plastic surgery in the future.
A Case of Complex Giant Rhinophyma Treated With Cosmetic Surgery
Authors: Hou Yikang , Zhu Ming , Chen Qiang , Tang Jieying , Zhang Shihong , Wang Chuan , Chen Lujia , Liao Xueyin , Li Weiwei , Yang Jianmin 
Published: June 2024

Abstract
Rhinophyma leads to severe facial deformities and significant social pressure for patients. Patients often seek medical intervention due to cosmetic defects and functional impairments, such as nasal congestion and airway collapse. Currently, there are numerous treatment modalities for rhinophyma, each with distinct advantages and disadvantages, leading to a lack of consensus in nasal vegetation management. Severe thickening in the nasal area can obstruct breathing through external nasal valve blockage, necessitating appropriate management for relief. This article presents a case study involving severe rhinophyma with respiratory obstruction that was successfully treated using incomplete resection followed by reconstruction to restore normal nasal contour. This not only achieved an upright position for nasal columella but also improved nasal contour to achieve normal appearance levels while completely relieving respiratory tract obstruction and enhancing patients' ventilation function. This method is easily performed without requiring additional expensive equipment, making it economically feasible even in ordinary medical centers while enabling patients to achieve a high quality of life.
Precision Drug Repurposing
Authors: Chen Shuwei , Zeng Junhao , Saad Mariam , Lineaweaver William C. , Chen Zhiwei , Pan Yuyan 
Published: June 2024

Background
Hyperpigmentation is a skin disorder characterized by a localized darkening of the skin due to increased melanin production. When patients fail first line topical treatments, secondary treatments such as chemical peels and lasers are offered. However, these interventions are not devoid of risks and are associated with postinflammatory hyperpigmentation. In the quest for novel therapeutic potentials, this study aims to investigate computational methods in the identification of new targeted therapies in the treatment of hyperpigmentation.

Methods
We used a comprehensive approach, which integrated text mining, interpreting gene lists through enrichment analysis and integration of diverse biological information (GeneCodis), protein-protein association networks and functional enrichment analyses (STRING), and plug-in network centrality parameters (Cytoscape) to pinpoint genes closely associated with hyperpigmentation. Subsequently, analysis of drug-gene interactions to identify potential drugs (Cortellis) was utilized to select drugs targeting these identified genes. Lastly, we used Deep Learning Based Drug Repurposing Toolkit (DeepPurpose) to conduct drug-target interaction predictions to ultimately identify candidate drugs with the most promising binding affinities.

Results
Thirty-four hyperpigmentation-related genes were identified by text mining. Eight key genes were highlighted by utilizing GeneCodis, STRING, Cytoscape, gene enrichment, and protein-protein interaction analysis. Thirty-five drugs targeting hyperpigmentation-associated genes were identified by Cortellis, and 29 drugs, including 16 M2PK1 inhibitors, 11 KRAS inhibitors, and 2 BRAF inhibitors were recommended by DeepPurpose.

Conclusions
The study highlights the promise of advanced computational methodology for identifying potential treatments for hyperpigmentation.
Prevalence of errors in lab-made plasmids across the globe
Authors: Bai Xingjian , Hong Jack F. , Yu Shan , Hu David Y. , Chen Amy Y. , Rich Constance A. , Shi Silk J. , Xu Sandy Y. , Croucher Daniel M. , Müssar Kristofer J. , Meng Daniel W. , Chen Jane L. , Lahn Bruce T. 
Published: June 2024

AbstractPlasmids are indispensable in life sciences research and therapeutics development. Currently, most labs custom-build their plasmids. As yet, no systematic data on the quality of lab-made plasmids exist. Here, we report a broad survey of plasmids from hundreds of academic and industrial labs worldwide. We show that nearly half of them contained design and/or sequence errors. For transfer plasmids used in making AAV vectors, which are widely used in gene therapy, about 40% carried mutations in ITR regions due to their inherent instability, which is influenced by flanking GC content. We also list genes difficult to clone into plasmid or package into virus due to their toxicity. Our finding raises serious concerns over the trustworthiness of lab-made plasmids, which parallels the underappreciated mycoplasma contamination and misidentified mammalian cell lines reported previously, and highlights the need for community-wide standards to uphold the quality of this ubiquitous reagent in research and medicine.