bluuebunny/crossref_metadata_2025_split huggingface dataset:
Population Genomic History of the Endangered Anatolian and Cyprian Mouflons in Relation to Worldwide Wild, Feral, and Domestic Sheep Lineages
Authors: Atağ Gözde , Kaptan Damla , Yüncü Eren , Başak Vural Kıvılcım , Mereu Paolo , Pirastru Monica , Barbato Mario , Leoni Giovanni Giuseppe , Güler Merve Nur , Er Tuğçe , Eker Elifnaz , Yazıcı Tunca Deniz , Kılıç Muhammed Sıddık , Altınışık Nefize Ezgi , Çelik Ecem Ayşe , Morell Miranda Pedro , Dehasque Marianne , Floridia Viviana , Götherström Anders , Bilgin Cemal Can , Togan İnci , Günther Torsten , Özer Füsun , Hadjisterkotis Eleftherios , Somel Mehmet 
Published: April 2024

Abstract
Once widespread in their homelands, the Anatolian mouflon (Ovis gmelini anatolica) and the Cyprian mouflon (Ovis gmelini ophion) were driven to near extinction during the 20th century and are currently listed as endangered populations by the International Union for Conservation of Nature. While the exact origins of these lineages remain unclear, they have been suggested to be close relatives of domestic sheep or remnants of proto-domestic sheep. Here, we study whole genome sequences of n = 5 Anatolian mouflons and n = 10 Cyprian mouflons in terms of population history and diversity, comparing them with eight other extant sheep lineages. We find reciprocal genetic affinity between Anatolian and Cyprian mouflons and domestic sheep, higher than all other studied wild sheep genomes, including the Iranian mouflon (O. gmelini). Studying diversity indices, we detect a considerable load of short runs of homozygosity blocks (&lt;2 Mb) in both Anatolian and Cyprian mouflons, reflecting small effective population size (Ne). Meanwhile, Ne and mutation load estimates are lower in Cyprian compared with Anatolian mouflons, suggesting the purging of recessive deleterious variants in Cyprian sheep under a small long-term Ne, possibly attributable to founder effects, island isolation, introgression from domestic lineages, or differences in their bottleneck dynamics. Expanding our analyses to worldwide wild and feral Ovis genomes, we observe varying viability metrics among different lineages and a limited consistency between viability metrics and International Union for Conservation of Nature conservation status. Factors such as recent inbreeding, introgression, and unique population dynamics may have contributed to the observed disparities.
Pain Lowers Subjective Survival Probabilities Among Middle-Aged and Older Adults
Authors: Fennell Gillian , Osuna Margarita , Ailshire Jennifer , Zajacova Anna 
Published: April 2024

Abstract

Objectives
Pain is a leading cause of disability and a limiting factor in individuals’ assessments of their own subjective health; however, its association with subjective longevity has yet to be explored. Subjective survival probabilities (SSPs), or one’s own perceived chances of living to a given age, can influence individuals’ behavior as they plan for their futures. This study assesses whether pain correlates to lower SSPs.

Methods
We use a repeated cross-section of the 2000–2018 waves of the Health and Retirement Study, a longitudinal and nationally representative survey of Americans aged 51 and older (N = 31,773).

Results
Fractional logit regressions indicate that, across all age groups, respondents with severe and/or interfering pain reported significantly lower SSPs than those with no pain (Marginal Effect [ME] = −0.03 to −0.06, p &lt; .05). Controlling for all covariates, mild or moderate noninterfering pain was only associated with a significant reduction in SSPs among the youngest group reporting their chances of living to age 75 (ME = −0.02, p &lt; .001). Descriptively and in the model results, respondents with mild or moderate noninterfering pain appeared to more closely resemble pain-free respondents than those with severe or interfering pain.

Discussion
These findings highlight the importance of pain on SSPs, and contribute to the growing evidence that pain interference is uniquely important in predicting meaningful health outcomes.
Socioeconomic and fiscal returns of expanded investment in immunization: a case for life-course vaccination in Colombia
Authors: Soto-Moreno Jose Alejandro , Coe Martha , Parellada Cintia , Tantri Anupama , Angarita-Contreras Maria Clara , Acosta Paula 
Published: April 2024

Abstract
Despite the health, societal, and economic benefits of immunization, many countries focus primarily on childhood immunizations and lack robust policies and sufficient resources for immunizations that can benefit populations across the life course. While the benefits of childhood vaccination are well documented, there is limited evidence on the financial and social return on investment that policymakers can use to inform decisions around administering a life-course immunization program. We developed a cost-benefit model from a societal perspective to evaluate the inclusion of 5 vaccines across the life course in Colombia's national immunization program. This model estimated a return of US$1.3 per US$1.0 invested in the first 2 decades, increasing to US$3.9 after 60 years. Primary benefits were productivity gains, followed by fiscal savings and household averted expenditure on health care. Furthermore, vulnerable households are predicted to receive 3.2 times greater income protection than formally employed households under a life-course immunization program. Consequently, there is a potential to reduce Colombia's income inequality and poverty rate by increasing access to immunization for all ages.
Aneurysmal artery supplying pulmonary sequestration: successful combined approach
Authors: Napolitano Antonio Giulio , Coviello Eleonora , Simonte Gioele , Vannucci Jacopo 
Published: April 2024

Abstract
A 62-year-old man was diagnosed with an asymptomatic intralobar pulmonary sequestration supplied by a short-neck proximal 57×25 mm aneurysmal artery originating from the mid-descending aorta. The patient underwent thoracic endovascular aneurysm repair; an aortic endograft was released to entirely restore the aberrant vessel. Then, pulmonary resection was performed. A triportal video-assisted left lower lobectomy was carried out. The thoracic endovascular aneurysm repair minimized the risk of bleeding and allowed a safe pulmonary resection with a minimally invasive approach.
Thermal and effective assessment of solar thermal air collector with roughened absorber surface: an analytical examination
Authors: Kumar Raj , Sethi Muneesh , Goel Varun , Ramis M K , AlSubih Majed , Islam Saiful , Al Ansari Mohammed Saleh , Lee Daeho , WogassoWodajo Anteneh 
Published: April 2024

Abstract
The current work analyses the thermal (ηth) and effective efficiency (${\eta}_{\mathrm{eff}}$) of a solar thermal air collector (STAC) that has an arc-shaped dimple as a roughness geometry on the absorber plate. Nusselt number (Nu) and friction factor (ff) were computed for roughness geometry during the testing, which was done on STAC. Additionally, for different roughness values, the correlations for Nu and ff were developed and further used in this study. The temperature rise parameter and a parametric design are used to assess these efficiencies. The influence of design variables on STAC performance is analyzed using a numerical model based on thermal and effective evaluations. During the investigation, parameters such as relative roughness height (e/Dh) varied from 0.021 to 0.036, relative roughness pitch (p/e) from 10 to 20, arc angle (α) from 45 to 60°, temperature rise parameter from 0.003 to 0.02 and Reynolds number (Re) from 3000 to 48 000 at a constant solar intensity (I = 1000 W/m2). The ηth and ${\eta}_{\mathrm{eff}}$ are observed to be 85% and 78%, respectively, at the optimum values of roughness parameters, i.e. e/Dh = 0.036, p/e = 10, and α = 60°. The curves have been plotted between each of the roughness parameters and Re in order to evaluate the best ηth and ${\eta}_{\mathrm{eff}}$ . The research emphasizes the usefulness of MATLAB for STAC analysis and optimization, roughness parameters of the suggested collector design, by integrating simulation and experimental data.
Good Words Are Worth Much, and Cost Little: Can Panel Maintenance Communication Be Effective in Increasing Survey Completion in a Volunteer Online Panel?
Authors: Kocar Sebastian 
Published: April 2024

Abstract
Online panels tend to be more affected by nonresponse bias than cross-sectional online and offline surveys due to an accumulating effect of attrition over time. Further, nonprobability panels that do not offer monetary rewards can be more sensitive to nonparticipation, but the existing research on panel maintenance strategies to increase survey completion in nonprobability/volunteer online panels is still scarce. To identify between-wave communications that could serve as cost-effective panel maintenance activities to increase survey completion, a survey experiment was carried out, and two solutions were empirically tested: sending survey results in a report (a long report or an infographic) and sending a prenotification before the following survey (a short or a longer prenotification). The results showed a small effect of sending an infographic, either with or without a short prenotification. Additionally, a positive effect was observed when emailing a short prenotification to those who completed the previous survey questionnaire, indicating a differential impact of between-wave communication. In summary, this study demonstrates how the survey completion rate can be increased in nonprobability online panels with non-monetary incentives, which can be incorporated into panel maintenance strategies for different types of panels.
Corticosterone Contributes to Diet-Induced Reprogramming of Post-Metamorphic Behavior in Spadefoot Toads
Authors: Shephard A M , Lagon S R , Jacobsen S , Millar K , Ledón-Rettig C C 
Published: April 2024

Synopsis
Stressful experiences in early life can have phenotypic effects that persist into, or manifest during, adulthood. In vertebrates, such carryover effects can be driven by stress-induced secretion of glucocorticoid hormones, such as corticosterone, which can lead to developmental reprogramming of hypothalamic-pituitary-adrenal/interrenal axis activity and behavior. Nutritional stress in the form of early life nutrient restriction is well known to modify later life behaviors and stress activity through corticosterone-related mechanisms. However, it is not known whether corticosterone is also mechanistically involved in carryover effects induced by a different form of nutritional variation: the use of alternate or entirely novel types of dietary resources. The plains spadefoot (Spea bombifrons) presents an excellent system for testing this question, since larvae of this species have evolved to use 2 alternate diet types: an ancestral detritus-based diet and a more novel diet of live shrimp. While previous work has shown that feeding on the novel shrimp diet influences juvenile (i.e., post-metamorphic) behavior and corticosterone levels, it is unclear whether these diet-induced carryover effects are mediated by diet-induced corticosterone itself. To test for the mechanistic role of corticosterone in diet-induced carryover effects, we experimentally treated S. bombifrons larvae with exogenous corticosterone and measured subsequent effects on juvenile behavior and corticosterone levels. We found that while shrimp-fed larvae had elevated corticosterone levels, treatment of larvae with corticosterone itself had effects on juvenile behavior that partially resembled those carryover effects induced by the shrimp diet, such as altered food seeking and higher locomotor activity. However, unlike carryover effects caused by the shrimp diet, larval corticosterone exposure did not affect juvenile corticosterone levels. Overall, our study shows that corticosterone-related mechanisms are likely involved in carryover effects induced by a novel diet, yet such diet-induced carryover effects are not driven by corticosterone alone.
Tundra wolf spider (Araneae: Lycosidae) abundance and phenology shift with distance from a northern highway
Authors: Gagnon Stéphanie , Scott Catherine E , Buddle Christopher M 
Published: April 2024

Abstract
Climate change is disproportionately impacting the North, and northern arthropods are particularly vulnerable to disturbances. The Dempster Highway is a gravel road that crosses the northern Yukon Territory in Canada, and wolf spiders (Araneae: Lycosidae) are the top arthropod predators on the tundra in this region. We investigated the effects of the Dempster Highway on subarctic tundra wolf spider abundance, phenology, and community composition by actively sampling 8 paired plots near (20 m) and far (200 m) from the road. Species richness did not differ with distance from the road, but total wolf spider abundance was much lower near the road, and this was driven by decreased abundance of the dominant tundra wolf spider species, Pardosa lapponica (Thorell, 1872). The overall proportion of females carrying egg sacs was higher near the road, suggesting advanced phenology in response to increased temperatures near the road. Together, these results indicate that the Dempster Highway impacts tundra wolf spider communities, which may have important consequences for food web dynamics and ecosystem function.
Les changements climatiques ont un impact plus important sur le Nord, et les arthropodes nordiques sont particulièrement vulnérables aux perturbations. La route Dempster est une route de gravier qui traverse le nord du territoire du Yukon au Canada, et les araignées-loups (Araneae: Lycosidae) sont d’importants prédateurs d’arthropodes de la toundra de cette région. Nous avons étudié les effets de la route Dempster sur l’abondance, la phénologie et la composition de la communauté des araignées-loups de la toundra subarctique en échantillonnant activement huit parcelles appariées proches (20 m) et éloignées (200 m) de la route. La richesse des espèces ne différait pas avec la distance par rapport à la route, mais l’abondance totale d’araignées-loups était inférieure près de la route, et cela peut être attribué à une diminution de l’abondance de l’espèce dominante d’araignées-loups de la toundra, Pardosa lapponica (Thorell, 1872). La proportion globale des femelles portant des cocons était plus élevée près de la route, suggérant une phénologie avancée en réponse à l’augmentation des températures près de la route. Ces résultats indiquent que, globalement, la route Dempster a un impact sur les communautés d’araignées-loups de la toundra, ce qui peut avoir des conséquences importantes sur la dynamique du réseau trophique et le fonctionnement de l’écosystème.
Nonelectroactive clostridium obtains extracellular electron transfer-capability after forming chimera with Geobacter
Authors: Liu Xing , Ye Yin , Yang Naiming , Cheng Chen , Rensing Christopher , Jin Chao , Nealson Kenneth H , Zhou Shungui 
Published: April 2024

Abstract
Extracellular electron transfer (EET) of microorganisms is a major driver of the microbial growth and metabolism, including reactions involved in the cycling of C, N, and Fe in anaerobic environments such as soils and sediments. Understanding the mechanisms of EET, as well as knowing which organisms are EET-capable (or can become so) is fundamental to electromicrobiology and geomicrobiology. In general, Gram-positive bacteria very seldomly perform EET due to their thick non-conductive cell wall. Here, we report that a Gram-positive Clostridium intestinale (C.i) attained EET-capability for ethanol metabolism only after forming chimera with electroactive Geobacter sulfurreducens (G.s). Mechanism analyses demonstrated that the EET was possible after the cell fusion of the two species was achieved. Under these conditions, the ethanol metabolism pathway of C.i was integrated by the EET pathway of G.s, by which achieved the oxidation of ethanol for the subsequent reduction of extracellular electron acceptors in the coculture. Our study displays a new approach to perform EET for Gram-positive bacteria via recruiting the EET pathway of an electroactive bacterium, which suggests a previously unanticipated prevalence of EET in the microbial world. These findings also provide new perspectives to understand the energetic coupling between bacterial species and the ecology of interspecies mutualisms.
Development of novel indole–quinoline hybrid molecules targeting bacterial proton motive force
Authors: Seo Jinbeom , Kim Ji-Hoon , Ko Nayoung , Kim Jihyeon , Moon Kyeongwon , Kim In Su , Lee Wonsik 
Published: April 2024

Abstract

Aims
This study aimed to develop an editable structural scaffold for improving drug development, including pharmacokinetics and pharmacodynamics of antibiotics by using synthetic compounds derived from a (hetero)aryl-quinoline hybrid scaffold.

Methods and results
In this study, 18 CF3-substituted (hetero)aryl-quinoline hybrid molecules were examined for their potential antibacterial activity against Staphylococcus aureus by determining minimal inhibitory concentrations. These 18 synthetic compounds represent modifications to key regions of the quinoline N-oxide scaffold, enabling us to conduct a structure-activity relationship analysis for antibacterial potency. Among the compounds, 3 m exhibited potency against with both methicillin resistant S. aureus strains, as well as other Gram-positive bacteria, including Enterococcus faecalis and Bacillus subtilis. We demonstrated that 3 m disrupted the bacterial proton motive force (PMF) through monitoring the PMF and conducting the molecular dynamics simulations. Furthermore, we show that this mechanism of action, disrupting PMF, is challenging for S. aureus to overcome. We also validated this PMF inhibition mechanism of 3 m in an Acinetobacter baumannii strain with weaken lipopolysaccharides. Additionally, in Gram-negative bacteria, we demonstrated that 3 m exhibited a synergistic effect with colistin that disrupts the outer membrane of Gram-negative bacteria.

Conclusions
Our approach to developing editable synthetic novel antibacterials underscores the utility of CF3-substituted (hetero)aryl-quinoline scaffold for designing compounds targeting the bacterial proton motive force, and for further drug development, including pharmacokinetics and pharmacodynamics.
Clinician perspectives on how situational context and augmented intelligence design features impact perceived usefulness of sepsis prediction scores embedded within a simulated electronic health record
Authors: Payne Velma L , Sattar Usman , Wright Melanie , Hill Elijah , Butler Jorie M , Macpherson Brekk , Jeppesen Amanda , Del Fiol Guilherme , Madaras-Kelly Karl 
Published: April 2024

Abstract

Objective
Obtain clinicians’ perspectives on early warning scores (EWS) use within context of clinical cases.

Material and Methods
We developed cases mimicking sepsis situations. De-identified data, synthesized physician notes, and EWS representing deterioration risk were displayed in a simulated EHR for analysis. Twelve clinicians participated in semi-structured interviews to ascertain perspectives across four domains: (1) Familiarity with and understanding of artificial intelligence (AI), prediction models and risk scores; (2) Clinical reasoning processes; (3) Impression and response to EWS; and (4) Interface design. Transcripts were coded and analyzed using content and thematic analysis.

Results
Analysis revealed clinicians have experience but limited AI and prediction/risk modeling understanding. Case assessments were primarily based on clinical data. EWS went unmentioned during initial case analysis; although when prompted to comment on it, they discussed it in subsequent cases. Clinicians were unsure how to interpret or apply the EWS, and desired evidence on its derivation and validation. Design recommendations centered around EWS display in multi-patient lists for triage, and EWS trends within the patient record. Themes included a “Trust but Verify” approach to AI and early warning information, dichotomy that EWS is helpful for triage yet has disproportional signal-to-high noise ratio, and action driven by clinical judgment, not the EWS.

Conclusions
Clinicians were unsure of how to apply EWS, acted on clinical data, desired score composition and validation information, and felt EWS was most useful when embedded in multi-patient views. Systems providing interactive visualization may facilitate EWS transparency and increase confidence in AI-generated information.
A specific and sensitive GC–MS-MS method for the quantitative determination of 2-phenoxyethanol and selected metabolites in human blood and urine
Authors: Jäger Thomas , Eckert Elisabeth , Leibold Edgar , Bader Michael 
Published: April 2024

Abstract
2-Phenoxyethanol (PhE) is widely used as a preservative in consumer products such as cosmetics as well as at the workplace as a component of metal-working fluids and hydraulic fluids. Therefore, both industry workers and consumers may potentially be exposed to PhE. An analytical method for the quantification of PhE and three selected metabolites, namely phenoxyacetic acid (PhAA), 4-hydroxyphenoxyacetic acid (4-OH-PhAA), and 4-hydroxyphenoxyethanol (4-OH-PhE), in human urine and blood was developed and validated. The sample preparation includes enzymatic hydrolysis of urine samples or protein precipitation of blood samples, followed by liquid–liquid extraction and silylation of the target analytes. Analyses of the extracts were carried out by gas chromatography with tandem mass spectrometry (GC–MS-MS). 3,4-Hydroxyphenoxyethanol, a probably minor PhE metabolite, could not be reliably analyzed due to its instability. The limits of quantification (LOQ) of the analytes ranged between 0.5 and 6.1 μg/L and 2.0 and 3.9 μg/L in urine and blood, respectively. The method was successfully applied to spot urine samples of 50 individuals without occupational exposure to PhE and additionally to blood samples from seven volunteers. In urine, PhAA and 4-OH-PhAA could be quantified in all analyzed samples, whereas 4-OH-PhE and unchanged PhE were found in 36% and 32% of the samples, respectively. In blood, PhAA was also found in every sample in levels above the LOQ, whereas PhE itself was detected in three of seven samples only. Neither 4-OH-PhAA nor 4-OH-PhE was found in any of the analyzed blood samples. The developed method promises to be a valuable tool for PhE monitoring of urine and blood samples and may also enable an advanced investigation of PhE biotransformation pathways in humans.