bluuebunny/crossref_metadata_2025_split huggingface dataset:
Leukocyte Immunoglobulin-Like Receptors Maintain Unique Antigen-Presenting Properties of Circulating Myeloid Dendritic Cells in HIV-1-Infected Elite Controllers
Authors: Huang Jinghe , Burke Patrick S. , Cung Thai Duong Hong , Pereyra Florencia , Toth Ildiko , Walker Bruce D. , Borges Luis , Lichterfeld Mathias , Yu Xu G. 
Published: July 2010

ABSTRACTElite controllers maintain undetectable levels of HIV-1 replication in the absence of antiretroviral therapy, but the correlates of immune protection in this patient population are ill defined. Here, we demonstrate that in comparison to patients with progressive HIV-1 infection or healthy persons not infected with HIV-1, elite controllers have circulating myeloid dendritic cells with significantly increased antigen-presenting properties, while their ability to secrete proinflammatory cytokines is substantially diminished. This unique functional profile is associated with a distinct surface expression pattern of immunomodulatoryleukocyte-immunoglobulin-likereceptors (LILR) and a strong and selective upregulation of LILRB1 and LILRB3. Blockade of these two receptors by monoclonal antibodies or short interfering RNA (siRNA) abrogated the specific antigen-presenting properties of dendritic cells, implying an important regulatory role of these molecules. These data reveal previously unrecognized innate components of immune protection against HIV-1 in elite controllers and offer novel perspectives for the manipulation of host immunity for the prevention and treatment of HIV-1 infection.
Evidence for Avian and Human Host Cell Factors That Affect the Activity of Influenza Virus Polymerase
Authors: Moncorgé Olivier , Mura Manuela , Barclay Wendy S. 
Published: July 2010

ABSTRACT
Typical avian influenza A viruses do not replicate efficiently in humans. The molecular basis of host range restriction and adaptation of avian influenza A viruses to a new host species is still not completely understood. Genetic determinants of host range adaptation have been found on the polymerase complex (PB1, PB2, and PA) as well as on the nucleoprotein (NP). These four viral proteins constitute the minimal set for transcription and replication of influenza viral RNA. It is widely documented that in human cells, avian-derived influenza A viral polymerase is poorly active, but despite extensive study, the reason for this blockade is not known. We monitored the activity of influenza A viral polymerases in heterokaryons formed between avian (DF1) and human (293T) cells. We have discovered that a positive factor present in avian cells enhances the activity of the avian influenza virus polymerase. We found no evidence for the existence of an inhibitory factor for avian virus polymerase in human cells, and we suggest, instead, that the restriction of avian influenza virus polymerases in human cells is the consequence of the absence or the low expression of a compatible positive cofactor. Finally, our results strongly suggest that the well-known adaptative mutation E627K on viral protein PB2 facilitates the ability of a human positive factor to enhance replication of influenza virus in human cells.
Varicella-Zoster Virus Immediate-Early Protein 62 Blocks Interferon Regulatory Factor 3 (IRF3) Phosphorylation at Key Serine Residues: a Novel Mechanism of IRF3 Inhibition among Herpesviruses
Authors: Sen Nandini , Sommer Marvin , Che Xibing , White Kris , Ruyechan William T. , Arvin Ann M. 
Published: July 2010

ABSTRACT
Varicella-zoster virus (VZV) is an alphaherpesvirus that is restricted to humans. VZV infection of differentiated cells within the host and establishment of latency likely require evasion of innate immunity and limited secretion of antiviral cytokines. Since interferons (IFNs) severely limit VZV replication, we examined the ability of VZV to modulate the induction of the type I IFN response in primary human embryonic lung fibroblasts (HELF). IFN-β production was not detected, and transcription of two interferon response factor 3 (IRF3)-dependent interferon-stimulated genes (ISGs), ISG54 and ISG56, in response to poly(I:C) stimulation was downregulated in VZV-infected HELF. Inhibition of IRF3 function did not require VZV replication; the viral immediate-early protein 62 (IE62) alone was sufficient to produce this effect. IE62 blocked TBK1-mediated IFN-β secretion and IRF3 function, as shown in an IFN-stimulated response element (ISRE)-luciferase reporter assay. However, IRF3 function was preserved if constitutively active IRF3 (IRF3-5D) was expressed in VZV-infected or IE62-transfected cells, indicating that VZV interferes with IRF3 phosphorylation. IE62-mediated inhibition was mapped to blocking phosphorylation of at least three serine residues on IRF3. However, IE62 binding to TBK1 or IRF3 was not detected and IE62 did not perturb TBK1-IRF3 complex formation. IE62-mediated inhibition of IRF3 function was maintained even if IE62 transactivator activity was disrupted. Thus, IE62 has two critical but discrete roles following VZV entry: to induce expression of VZV genes and to disarm the IFN-dependent antiviral defense through a novel mechanism that prevents IRF3 phosphorylation.
Annexin 2 Is Not Required for Human Immunodeficiency Virus Type 1 Particle Production but Plays a Cell Type-Dependent Role in Regulating Infectivity
Authors: Rai Tia , Mosoian Arevik , Resh Marilyn D. 
Published: July 2010

ABSTRACT

During assembly and budding of retroviruses, host cell proteins are incorporated into viral particles. Identification of virion-associated proteins may help pinpoint key cellular components required for virus production and function. The cellular protein annexin 2 (Anx2) is incorporated into HIV-1 particles, and knockdown of Anx2 has been reported to cause defects in Gag processing and infectivity of HIV-1 particles in macrophages. Here, we tested whether Anx2 was required for HIV-1 production in other cell types capable of producing HIV-1 virions. Endogenous Anx2 levels were knocked down by ∼98% using lentivirus encoding short hairpin RNAs (shRNAs) or small interfering RNAs (siRNAs) targeting Anx2. Under these conditions, there was no reduction in HIV-1 virus-like particle (VLP) production in either COS-1, 293T, or Jurkat T cells or primary human monocyte-derived macrophages (MDMs). Murine embryonic fibroblasts derived from Anx2
−/−
mice produced the same levels of VLPs as matched cells from wild-type mice. The calcium-mediated spike in VLP production still occurred in Anx2-depleted COS-1 cells, and there was no apparent alteration in the intracellular Gag localization. Overexpression of Anx2 in
trans
had no effect on Gag processing or VLP production. Neither Anx2 depletion nor Anx2 overexpression altered the infectivity of HIV-1 particles produced by COS-1 or 293T cells. However, supernatants containing virus from Anx2 siRNA-treated primary human MDMs exhibited decreased infectivity. These data indicate that Anx2 is not required for HIV-1 assembly or Gag processing but rather plays a cell type-dependent role in regulating production of infectious HIV-1 by macrophages.
Late-night salivary cortisol in normal subjects and in patients with Cushing’s syndrome
Authors: Jeyaraman K , Ammini A C , Nandita G , Dwivedi S N 
Published: July 2010

Abstract

Background
Late-night salivary cortisol is used as a screening test for Cushing’s syndrome (CS) in many European and American countries. However, its utility has not been studied in an Asian-Indian population.

Objective
To establish the reference range in Asian-Indians and to evaluate its usefulness in the diagnosis of CS.

Methods
Three groups of subjects were studied: normal subjects, patients with suspected CS, and patients with proven CS. All participants collected saliva at 23:00 h using a Salivette. Salivary cortisol was measured using an automated electrochemiluminescence assay.

Results
There were 56 normal subjects, 40 patients with suspected CS, and 30 with proven CS. Of the 40 with suspected CS, three were confirmed to have CS. The remaining 37 served as control patients. The 97.5th centile of the late-night salivary cortisol concentrations in normal subjects was 10.87 nmol/l. The mean±SD 23:00 h salivary cortisol concentration in control patients and those with confirmed CS was 3.21±2.36 nmol/l and 32.33±44.14 nmol/l, respectively. All the control patients and 30.3% (10/33) of patients with CS had a salivary cortisol concentration of &lt;10.87 nmol/l. With the use of a receiver operating characteristic curve, a cut-off of 4.55 nmol/l gave a sensitivity of 93.9% and specificity of 81.1%. However, as this cut-off is less than the functional sensitivity of the assay, it may not be clinically applicable.

Conclusions
The reference range for late-night salivary cortisol in our population was &lt;10.87 nmol/l. With this cut-off, the sensitivity was 69.2% and specificity 100%. Even though this automated electrochemiluminescence assay is easy and quick to use, its clinical utility in measuring the low salivary cortisol concentrations needs further investigation.
Feasibility and safety of outpatient radiofrequency catheter ablation procedures for atrial fibrillation
Authors: Haegeli Laurent M , Duru Firat , Lockwood Evan E , Lüscher Thomas F , Sterns Laurence D , Novak Paul G , Leather Richard A 
Published: July 2010

Abstract

Introduction
Percutaneous catheter ablation for atrial fibrillation (AF) is a procedure performed typically in an inpatient setting. The feasibility and safety of catheter ablation in patients with paroxysmal and persistent AF were evaluated on an outpatient basis.

Methods
230 AF ablation procedures were performed in 206 patients (74% male; mean age 56±9 years). Patients were admitted to the hospital outpatient facility in the morning for the AF ablation procedure on the same day. The ablation strategy consisted of wide area circumferential lines around both ipsilateral pulmonary veins. After monitoring in the outpatient service, patients were discharged on the same day, if they were clinically stable.

Results
Mean procedure time was 201±31 min. Major complications occurred in seven patients (3%). One patient (0.4%) suffered a minor stroke and six patients had pericardial tamponade requiring percutaneous drainage. Patients could be discharged on the same day following 205 (89%) procedures. Among the 148 patients whose clinical outcome was assessed at 6 months, 127 (86%) had a reduction of the total symptomatic AF episodes, compared to pre-ablation, with a complete lack of symptoms in 101 patients (68%).

Conclusion
Catheter ablation of AF on the day of admission is feasible and safe with a low risk of complications. The vast majority of the patients can be discharged on the same day.
ON THE QUANTUM COMPLEXITY OF EVALUATING THE TUTTE POLYNOMIAL
Authors: AHMADI HAMED , WOCJAN PAWEL 
Published: July 2010

We show that the problem of approximately evaluating the Tutte polynomial of triangular graphs at the points (q, 1/q) of the Tutte plane is BQP-complete for (most) roots of unity q. We also consider circular graphs and show that the problem of approximately evaluating the Tutte polynomial of these graphs at the point (e2πi/5, e-2πi/5) is DQC1-complete and at points [Formula: see text] for some integer k is in BQP. To show that these problems can be solved by a quantum computer, we rely on the relation of the Tutte polynomial of a planar G graph with the Jones and HOMFLY polynomial of the alternating link D(G) given by the medial graph of G. In the case of our graphs the corresponding links are equal to the plat and trace closures of braids. It is known how to evaluate the Jones and HOMFLY polynomial for closures of braids. To establish the hardness results, we use the property that the images of the generators of the braid group under the irreducible Jones–Wenzl representations of the Hecke algebra have finite order. We show that for each braid b we can efficiently construct a braid [Formula: see text] such that the evaluation of the Jones and HOMFLY polynomials of their closures at a fixed root of unity leads to the same value and that the closures of [Formula: see text] are alternating links.
High-quality single-shot capture of facial geometry
Authors: Beeler Thabo , Bickel Bernd , Beardsley Paul , Sumner Bob , Gross Markus 
Published: July 2010

This paper describes a passive stereo system for capturing the 3D geometry of a face in a single-shot under standard light sources. The system is low-cost and easy to deploy. Results are submillimeter accurate and commensurate with those from state-of-the-art systems based on active lighting, and the models meet the quality requirements of a demanding domain like the movie industry. Recovered models are shown for captures from both high-end cameras in a studio setting and from a consumer binocular-stereo camera, demonstrating scalability across a spectrum of camera deployments, and showing the potential for 3D face modeling to move beyond the professional arena and into the emerging consumer market in stereoscopic photography.
Our primary technical contribution is a modification of standard stereo refinement methods to capture pore-scale geometry, using a qualitative approach that produces visually realistic results. The second technical contribution is a calibration method suited to face capture systems. The systemic contribution includes multiple demonstrations of system robustness and quality. These include capture in a studio setup, capture off a consumer binocular-stereo camera, scanning of faces of varying gender and ethnicity and age, capture of highly-transient facial expression, and scanning a physical mask to provide ground-truth validation.
A radiative transfer framework for rendering materials with anisotropic structure
Authors: Jakob Wenzel , Arbree Adam , Moon Jonathan T. , Bala Kavita , Marschner Steve 
Published: July 2010

The radiative transfer framework that underlies all current rendering of volumes is limited to scattering media whose properties are invariant to rotation. Many systems allow for "anisotropic scattering," in the sense that scattered intensity depends on the scattering angle, but the standard equation assumes that the structure of the medium is isotropic. This limitation impedes physics-based rendering of volume models of cloth, hair, skin, and other important volumetric or translucent materials that do have anisotropic structure. This paper presents an end-to-end formulation of physics-based volume rendering of anisotropic scattering structures, allowing these materials to become full participants in global illumination simulations.
We begin with a generalized radiative transfer equation, derived from scattering by oriented non-spherical particles. Within this framework, we propose a new volume scattering model analogous to the well-known family of microfacet surface reflection models; we derive an anisotropic diffusion approximation, including the weak form required for finite element solution and a way to compute the diffusion matrix from the parameters of the scattering model; and we also derive a new anisotropic dipole BSSRDF for anisotropic translucent materials. We demonstrate results from Monte Carlo, finite element, and dipole simulations. All these contributions are readily implemented in existing rendering systems for volumes and translucent materials, and they all reduce to the standard practice in the isotropic case.
Star-contours for efficient hierarchical self-collision detection
Authors: Schvartzman Sara C. , Pérez Álvaro G. , Otaduy Miguel A. 
Published: July 2010

Collision detection is a problem that has often been addressed efficiently with the use of hierarchical culling data structures. In the subproblem of self-collision detection for triangle meshes, however, such hierarchical data structures lose much of their power, because triangles adjacent to each other cannot be distinguished from actually colliding ones unless individually tested. Shape regularity of surface patches, described in terms of orientation and contour conditions, was proposed long ago as a culling criterion for hierarchical self-collision detection. However, to date, algorithms based on shape regularity had to trade conservativeness for efficiency, because there was no known algorithm for efficiently performing 2D contour self-intersection tests.
In this paper, we introduce a star-contour criterion that is amenable to hierarchical computations. Together with a thorough analysis of the tree traversal process in hierarchical self-collision detection, it has led us to novel hierarchical data structures and algorithms for efficient yet conservative self-collision detection. We demonstrate the application of our algorithm to several example animations, and we show that it consistently outperforms other approaches.
Unstructured video-based rendering
Authors: Ballan Luca , Brostow Gabriel J. , Puwein Jens , Pollefeys Marc 
Published: July 2010

We present an algorithm designed for navigating around a performance that was filmed as a "casual" multi-view video collection: real-world footage captured on hand held cameras by a few audience members. The objective is to easily navigate in 3D, generating a video-based rendering (VBR) of a performance filmed with widely separated cameras. Casually filmed events are especially challenging because they yield footage with complicated backgrounds and camera motion. Such challenging conditions preclude the use of most algorithms that depend on correlation-based stereo or 3D shape-from-silhouettes.
Our algorithm builds on the concepts developed for the exploration of photo-collections of empty scenes. Interactive performer-specific view-interpolation is now possible through innovations in interactive rendering and offline-matting relating to i) modeling the foreground subject as video-sprites on billboards, ii) modeling the background geometry with adaptive view-dependent textures, and iii) view interpolation that follows a performer. The billboards are embedded in a simple but realistic reconstruction of the environment. The reconstructed environment provides very effective visual cues for spatial navigation as the user transitions between viewpoints. The prototype is tested on footage from several challenging events, and demonstrates the editorial utility of the whole system and the particular value of our new inter-billboard optimization.
Filament-based smoke with vortex shedding and variational reconnection
Authors: Weißmann Steffen , Pinkall Ulrich 
Published: July 2010

Simulating fluids based on vortex filaments is highly attractive for the creation of special effects because it gives artists full control over the simulation using familiar tools like curve editors or the scripted generation of new vortex filaments over time. Because filaments offer a very compact description of fluid flow, real time applications like games or virtual reality are also possible.
We present a complete model that includes moving obstacles with vortex shedding, all represented as filaments. Due to variational reconnection the long-time behavior of our method is excellent: Energy and momentum stay constant within reasonable bounds and computational complexity does not increase over time.
Feature-aligned T-meshes
Authors: Myles Ashish , Pietroni Nico , Kovacs Denis , Zorin Denis 
Published: July 2010

High-order and regularly sampled surface representations are more efficient and compact than general meshes and considerably simplify many geometric modeling and processing algorithms. A number of recent algorithms for conversion of arbitrary meshes to regularly sampled form (typically quadrangulation) aim to align the resulting mesh with feature lines of the geometry. While resulting in a substantial improvement in mesh quality, feature alignment makes it difficult to obtain coarse regular patch partitions of the mesh.
In this paper, we propose an approach to constructing patch layouts consisting of small numbers of quadrilateral patches while maintaining good feature alignment. To achieve this, we use quadrilateral T-meshes, for which the intersection of two faces may not be the whole edge or vertex, but a part of an edge. T-meshes offer more flexibility for reduction of the number of patches and vertices in a base domain while maintaining alignment with geometric features. At the same time, T-meshes retain many desirable features of quadrangulations, allowing construction of high-order representations, easy packing of regularly sampled geometric data into textures, as well as supporting different types of discretizations for physical simulation.
Superposition for fixed domains
Authors: Horbach Matthias , Weidenbach Christoph 
Published: July 2010

Superposition is an established decision procedure for a variety of first-order logic theories represented by sets of clauses. A satisfiable theory, saturated by superposition, implicitly defines a minimal term-generated model for the theory. Proving universal properties with respect to a saturated theory directly leads to a modification of the minimal model's term-generated domain, as new Skolem functions are introduced. For many applications, this is not desired.
Therefore, we propose the first superposition calculus that can explicitly represent existentially quantified variables and can thus compute with respect to a given domain. This calculus is sound and refutationally complete in the limit for a first-order fixed domain semantics. For saturated Horn theories and classes of positive formulas, we can even employ the calculus to prove properties of the minimal model itself, going beyond the scope of known superposition-based approaches.
Receptor Activator of NF-κB Ligand Enhances Breast Cancer–Induced Osteolytic Lesions through Upregulation of Extracellular Matrix Metalloproteinase Inducer/CD147
Authors: Rucci Nadia , Millimaggi Danilo , Mari Marianna , Del Fattore Andrea , Bologna Mauro , Teti Anna , Angelucci Adriano , Dolo Vincenza 
Published: July 2010

Abstract
Breast cancer shows a strong predilection to metastasize to bone. Cell surface glycoprotein extracellular matrix metalloproteinase inducer (EMMPRIN)/CD147 induces metalloproteinases (MMP) and vascular endothelial growth factor (VEGF), which may support osteoclastic activity and increased incidence of breast cancer bone metastases. In support of this hypothesis, we observed that MDA-MB-231 human breast tumor cells engineered to overexpress EMMPRIN strongly induced osteolytic lesions in immunodeficient mice, which was blunted by in vivo treatment with an EMMPRIN blocking antibody. Similarly, these cells exhibited increased expression of MMP-9 and VEGF relative to control cells. Treatment of MDA-MB-231 cells with the osteoclastogenic cytokine receptor activator of NF-κB ligand (RANKL) upregulated EMMPRIN expression with a parallel increase of MMP-9 and VEGF. Conditioned medium from osteoblasts similarly increased EMMPRIN, MMP-9, and VEGF expression in cells. Osteoblast treatment with the RANKL decoy receptor osteoprotegerin abolished this effect. EMMPRIN overexpression stimulated MDA-MB-231 cell invasion but not proliferation. Conversely, small interfering RNA–mediated knockdown of EMMPRIN downregulated MMP-9 and VEGF basal expression and RANKL-stimulated expression, and reduced cell invasion. Our results argue that EMMPRIN drives breast cancer–induced osteolytic lesions and that activation of the RANKL pathway increases EMMPRIN in osteotropic tumor cells, in turn enhancing tumor-induced bone resorption. Cancer Res; 70(15); 6150–60. ©2010 AACR.
Monitoring of Natural Killer Cell Immunotherapy Using Noninvasive Imaging Modalities
Authors: Jha Priyanka , Golovko Daniel , Bains Sukhmine , Hostetter Daniel , Meier Reinhard , Wendland Michael F. , Daldrup-Link Heike E. 
Published: July 2010

Abstract
Cancer immunotherapies can be guided by cellular imaging techniques, which can identify the presence or absence of immune cell accumulation in the tumor tissue in vivo and in real time. This review summarizes various new and evolving imaging techniques employed for tracking and monitoring of adoptive natural killer cell immunotherapies. Cancer Res; 70(15); 6109–13. ©2010 AACR.