bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endoscopic injection sclerotherapy for esophageal varices associated with concomintant portal venous thrombus of hepatocellular carcinoma
Authors: Ohta Masayuki , Hashizume Makoto , Tomikawa Morimasa , Kamakura Tatsuro , Akazawa Kouhei , Ueno Kiichiro , Yamaga Hirohiko , Kitano Seigo , Tanoue Kazuo , Matsumata Takashi , Sugimachi Keizo 
Published: June 2010

AbstractBetween 1983 and 1994, we treated 51 patients with esophageal varices and portal trunk and main branch invasion of hepatocellular carcinoma, using endoscopic injection sclerotherapy. Variceal bleeding was controlled in 28 of 29 patients (96.6%), esophageal varices were completely eradicated in 28 (54.9%), and only 2 of 28 (7.1%) bled from small, dilated, venous vessels after eradication. The cumulative nonbleeding rate at 3 years was 87.5%. Death caused by hepatocellular carcinoma accounted for 89.4% of the patients, whereas the rate of bleeding from esophageal varices was 4.3%. Variables significantly associated with the duration of survival were Okuda's clinical stage, alpha‐fetoprotein, eradication of esophageal varices by sclerotherapy, and treatment of hepatocellular carcinoma, as determined in a univariate analysis. Multivariate analysis showed that eradication of esophageal varices by sclerotherapy, Okuda's clinical stage, and age were independent factors which significantly influenced survival time. We propose that complete eradication of esophageal varices and close follow‐up using endoscopy may lead to a reduction in bleeding from esophageal varices, and hence may reduce mortality rates related to this bleeding.
Concomitant sclerosing mesenteritis and inflammatory pseudotumor simulating gastric lymphoma or linitis plastica
Authors: Gincherman Yevgeniy , Langer Jille E. , Miller Leonard 
Published: June 2010

AbstractBoth inflammatory pseudotumours and sclerosing (retractile) mesenteritis are uncommon conditions of unknown origin. A number of authors have reported patients presenting with complaints that were attributed to one or the other of these entities. In our review of the literature, we were unable to find a single case report of mixed abdominal findings that could be explained by the presence of both conditions. We report a case of the patient presenting with abdominal mass that was identified as having features of both sclerosing mesenteritis and inflammatory pseudotumor on pathologic diagnosis.
Spindle cell tuor of the portal vein
Authors: Madariaga Juan R. , Nalesnik Michael A. , Iwatsuki Shunzaburo , Starzl Thomas E. , Fung John J. 
Published: June 2010

AbstractThe authors describe the first reported case of spindle cell tumor of the portal vein (SCTPV) surgically excised in a 28‐year‐old woman. The tumor had caused portal vein obstruction with extensive collaterals. A side‐to‐side mesocaval shunt was created to reduce portal hypertension. Six months later the SCTPV could be excised. Radiation therapy (4,500 cGY) was given 2 months later. Although the tumor recurred in the liver 4 years later, the patient is alive and free of symptoms 6 years after the initial diagnosis. © 1995 Wiley‐Liss, Inc.
Application of nanofiber‐packed SPE for determination of salivary‐free cortisol using fluorescence precolumn derivatization and HPLC detection
Authors: Chen Li‐Qin , Kang Xue‐Jun , Sun Jing , Deng Jian‐Jun , Gu Zhong‐Ze , Lu Zu‐Hong 
Published: June 2010

AbstractSalivary cortisol has emerged as an easy‐to‐collect biologic marker of stress in many researches. In this study, we present a method for the determination of salivary‐free cortisol using HPLC method with fluorescence precolumn derivatization, which is based on a novel extraction from the strongly acidic medium (fluorescent derivatives of cortisol in sulfuric acid medium) by electrospun polystyrene nanofibers packed SPE. For high‐throughput sample extraction, an array pretreatment device based on nanofibers packed SPE micro‐column was designed. The LOD of cortisol was 0.01 μg/L (S/N=3). The RSDs (n=6) for all analytes were below 8.0%, and the recoveries were 110, 102.4, and 99.4% (n=3) for saliva spiked with 0.1, 10, and 20 μg/L of cortisol, respectively. The proposed method was then successfully applied in the determination of free cortisol in human saliva. The salivary cortisol concentrations in the real samples ranged from 0.22 to 7.45 μg/L. The nanofiber‐packed SPE overcame the low extraction recovery and bad clean‐up effect of the conventional methods, and increased the sensitivity and selectivity of the method.
Denaturing capillary electrophoresis for automated detection of L858R mutation in exon 21 of the epidermal growth factor receptor gene in prediction of the outcome of lung cancer therapy
Authors: Benesova Lucie , Pesek Milos , Belsanova Barbora , Sekerka Patrik , Minarik Marek 
Published: June 2010

AbstractThe presence of activating mutations within the tyrosine kinase domain of the epidermal growth factor receptor gene has been attributed to a positive response to biological therapy of lung cancer by small‐molecular tyrosine kinase inhibitors, gefitinib and erlotinib. Among the two most significant mutation types are deletions in exon 19 and a single point substitution in exon 21 (termed L858R). The exon 19 deletions can readily be examined by fragment analysis, due to the characteristic length difference between the normal and mutated PCR product. Analysis of the L858R point mutation, however, presents a greater challenge. The current paper is aimed at developing a sensitive, yet simple, low‐cost mutation detection assay directed at the L858R mutation using a method based on CE of heteroduplexes under partial denaturing conditions. We perform optimization of separation conditions on different commercial instruments including ones equipped with 8, 16 and 96 capillaries. We present normalized migration reproducibility in the range from 1 (8 and 16) to 5% (96) RSD. A reliable distinction of the R836R silent polymorphism from a potential presence of the L858R mutation is also demonstrated. In its implementation, the presented assay is just another application running on a conventional CE platform without the need of dedicated instrumentation.
HPLC‐PAD‐atmospheric pressure chemical ionization‐MS metabolite profiling of cytotoxic carotenoids from the echinoderm Marthasterias glacialis (spiny sea‐star)
Authors: Ferreres Federico , Pereira David M. , Gil‐Izquierdo Angel , Valentão Patrícia , Botelho João , Mouga Teresa , Andrade Paula B. 
Published: June 2010

AbstractAn HPLC‐PAD‐atmospheric pressure chemical ionization‐MS metabolite profiling analysis was conducted on the marine echinoderm Marthasterias glacialis (spiny sea‐star). Bio‐guided purification of the methanolic extract led to the isolation of several carotenoids, namely zeaxanthin, astaxanthin and lutein. These compounds were characterized using both UV–Vis characteristics and MS spectra interpretation. No previous works addressed the MS analysis of carotenoids present in this organism. The purified carotenoid fraction displayed a strong cell proliferation inhibition against rat basophilic leukemia RBL‐2H3 (IC25=268 μg/mL) cancer cell line. Against healthy V79 (rat lung fibroblasts (IC25=411 μg/mL)) cell line, however, toxicity was lower, as it is desired for anti‐cancer molecules. This study suggests that M. glacialis may constitute a good source of bioactive compounds that can be used as lead compounds for the pharmaceutical industry.
Efficient isolation and purification of five products from microbial biotransformation of cinobufagin by high‐speed counter‐current chromatography
Authors: Ma Xiao‐Chi , Xin Xiulan , Zhang Bao‐jing , Deng Sha , Yao Ji‐hong , Wang Chang‐yuan , Cui Jian , Tian Yan , Liu Ke‐xin 
Published: June 2010

AbstractAn efficient separation method of using high‐speed counter‐current chromatography was successfully established to directly purify cytotoxic transformed products of cinobufagin byCordyceps militaris. The two‐phase solvent system composed ofn‐hexane–ethyl acetate–methanol–water (4:6:3:4, v/v) was used in high‐speed counter‐current chromatography. A total of 9 mg of 4β,12α‐dihydroxyl‐cinobufagin (1), 15 mg of 12β‐hydroxyl‐cinobufagin (2), 8 mg of 5β‐hydroxyl‐cinobufagin (3), 12 mg of deacetylcinobufagin (4) and 6 mg of 3‐keto‐cinobufagin (5) were obtained in a one‐step separation from 400 mg of the crude extract with purity of 98.7, 97.2, 90.6, 99.1 and 99.4%, respectively, as determined by HPLC. Their chemical structures were identified on the basis of1H‐NMR and13C‐NMR technology. All products (1–5) showed the potent activities against human carcinoma cervicis (Hela) and malignant melanoma (A375) cellsin vitro.
A technique for detecting GABA in the human brain with PRESS localization and optimized refocusing spectral editing radiofrequency pulses
Authors: Keltner John R. , Wald Lawrence L. , Christensen James D. , Maas Luis C. , Moore Constance M. , Cohen Bruce M. , Renshaw Perry F. 
Published: June 2010

AbstractA proton magnetic resonance spectral editing technique is presented that uses PRESS excitation to achieve spatially localized measurements of brain gamma‐aminobutyric acid (GABA). The homonuclear difference spectroscopy technique employs a frequency selective inversion pulse to suppress the creatine resonance at 3.0 ppm. The timing of this pulse is optimized to maximize the suppression of creatine by minimizing the effect of the editing pulse on the 3.0 ppm resonances. The PRESS excitation achieves three dimensional spatial localization in a single acquisition making it less sensitive to patient motion than multiple acquisition techniques. The performance and utility of this technique were evaluated by phantom experiments and by in vivo measurements of brain GABA concentration in 10 normal subjects.
Comparison of k‐space sampling schemes for multidimensional MR spectroscopic imaging
Authors: Hugg James W. , Maudsley Andrew A. , Weiner Michael W. , Matson Gerald B. 
Published: June 2010

AbstractFor clinical 31P MR spectroscopic imaging (MRSI) studies, where signal averaging is necessary, some improvement of sensitivity and spatial response function may be achieved by acquiring data over a spherical k‐space volume and varying the number of averages acquired in proportion to the desired spatial filter. Eight different k‐space sampling schemes are compared through simulations that provide graphs of the spatial response functions (SRF), and tabulations of voxel volumes, relative signal‐to‐noise ratios (SNR), and relative data collection efficiencies (SNR per unit volume over the same time). All schemes were based on practical experiments, each of which could be implemented in the same length of time. The results show that in comparison with cubic k‐space sampling with the same number of signal averages at each point, spherical and acquisition‐weighted k‐space sampling can be used to achieve reduced Gibbs ringing along the principal axes directions, and thus reduced contamination from adjacent tissue in these directions, without degradation of voxel volume or SNR.
Power efficient on‐resonance saturation pulses for magnetization transfer in magnetic resonance imaging
Authors: Mao Jintong , Ballinger J. Ray 
Published: June 2010

AbstractA family of new on‐resonance saturation pulses for magnetization transfer in MRI is proposed. These pulses can be represented as a product of a shaped function and a cosine function. The shaped function can have many different forms, one of which is a Gaussian function. The experimental results on a 1.0 T whole body scanner show that the new on‐resonance pulses are more efficient for magnetization transfer than either on‐resonance binomial sequence pulses or offresonance Gaussian pulses at the same power level.
Practical implementation of single‐voxel double‐quantum editing on a whole‐body NMR spectrometer: Localized monitoring of lactate in the human leg during and after exercise
Authors: Jouvensal Laurence , Carlier Pierre G. , Bloch Gilles 
Published: June 2010

AbstractThe classical double‐quantum editing sequence 90°x‐τ−180°y‐τ−90°x‐t1−90°x‐τ−180°y‐τ‐AQ (τ = 1/4J) was rendered volume selective, by making slice selective the first 90° pulse and the two 180° pulses. Using simple rules to ensure optimum radio frequency phase coherence, this single‐voxel editing sequence, reminiscent of a basic PRESS localization technique, was implemented on a whole‐body 3 T spectrometer, and in vitro editing of lactate methyl protons was demonstrated without any significant loss in intrinsic sensitivity. The effectiveness of the proposed approach in vivo was also illustrated through the localized monitoring of lactate in the human leg during and after exercise.
Reduction of errors in ASL cerebral perfusion and arterial transit time maps using image de‐noising
Authors: Wells Jack A. , Thomas David L. , King Martin D. , Connelly Alan , Lythgoe Mark F. , Calamante Fernando 
Published: June 2010

AbstractIn this work, the performance of image de‐noising techniques for reducing errors in arterial spin labeling cerebral blood flow and arterial transit time estimates is investigated. Simulations were used to show that the established arterial spin labeling cerebral blood flow quantification method exhibits the bias behavior common to nonlinear model estimates, and as a result, the reduction of random errors using image de‐noising can improve accuracy. To assess the effect on precision, multiple arterial spin labeling data sets acquired from the rat brain were processed using a variety of common de‐noising methods (Wiener filter, anisotropic diffusion filter, gaussian filter, wavelet decomposition, and independent component analyses). The various de‐noising schemes were also applied to human arterial spin labeling data to assess the possible extent of structure degradation due to excessive spatial smoothing. The animal experiments and simulated data show that noise reduction methods can suppress both random and systematic errors, improving both the precision and accuracy of cerebral blood flow measurements and the precision of transit time maps. A number of these methods (and particularly independent component analysis) were shown to achieve this aim without compromising image contrast. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.
Three‐dimensional ultrasound in evaluating the fetus
Authors: Dückelmann Anna M. , Kalache Karim D. 
Published: June 2010

AbstractIn recent years three‐dimensional (3D) ultrasound has made a place in clinical practice and has become a major field of research in obstetrics. In this article we will review the diagnostic performance of the most widely used 3D ultrasound applications in the assessment of fetal anomalies, explain the technique to gain correct 3D images and offer some practical advice for their efficient use. Examples are given to demonstrate the applicability and vividness of 3D in daily routine. Copyright © 2010 John Wiley & Sons, Ltd.
NMR and crystallographic structures of the FK506 binding domain of human malarial parasite Plasmodium vivax FKBP35
Authors: Alag Reema , Qureshi Insaf A. , Bharatham Nagakumar , Shin Joon , Lescar Julien , Yoon Ho Sup 
Published: June 2010

AbstractThe emergence of drug‐resistant malaria parasites is the major threat to effective malaria control, prompting a search for novel compounds with mechanisms of action that are different from the traditionally used drugs. The immunosuppressive drug FK506 shows an antimalarial activity. The mechanism of the drug action involves the molecular interaction with the parasite target proteins PfFKBP35 and PvFKBP35, which are novel FK506 binding protein family (FKBP) members from Plasmodium falciparum and Plasmodium vivax, respectively. Currently, molecular mechanisms of the FKBP family proteins in the parasites still remain elusive. To understand their functions, here we have determined the structures of the FK506 binding domain of Plasmodium vivax (PvFKBD) in unliganded form by NMR spectroscopy and in complex with FK506 by X‐ray crystallography. We found out that PvFKBP35 exhibits a canonical FKBD fold and shares kinetic profiles similar to those of PfFKBP35, the homologous protein in P. falciparum, indicating that the parasite FKBP family members play similar biological roles in their life cycles. Despite the similarity, differences were observed in the ligand binding modes between PvFKBD and HsFKBP12, a human FKBP homolog, which could provide insightful information into designing selective antimalarial drug against the parasites.
Interaction of an ionic complementary peptide with a hydrophobic graphite surface
Authors: Sheng Yuebiao , Wang Wei , Chen P. 
Published: June 2010

AbstractProtein adsorption on a surface plays an important role in biomaterial science and medicine. It is strongly related to the interaction between the protein residues and the surface. Here we report all‐atom molecular dynamics simulations of the adsorption of an ionic complementary peptide, EAK16‐II, to the hydrophobic highly ordered pyrolytic graphite surface. We find that, the hydrophobic interaction is the main force to govern the adsorption, and the peptide interchain electrostatic interaction affects the adsorption rate. Under neutral pH condition, the interchain electrostatic attraction facilitates the adsorption, whereas under acidic and basic conditions, because of the protonation and deprotonation of glutamic acid and lysine residues, respectively, the resulting electrostatic repulsion slows down the adsorption. We also found that under basic condition, during the adsorption peptide Chain II will be up against a choice to adsorb to the surface through the hydrophobic interaction or to form a temporary hydrophobic core with the deposited peptide Chain I. These results provide a basis for understanding some of the fundamental interactions governing peptide adsorption on the surface, which can shed new light on novel applications, such as the design of implant devices and drug delivery materials.
The Total Chemical Synthesis of Monocyte Chemotactic Protein‐1 (MCP‐1)
Authors: Brown Angus R. , Covington Maryanne , Newton Robert C. , Ramage Robert , Welch Patricia 
Published: June 2010

AbstractThe affinity‐based Nα‐amino protecting group tetrabenzo [a,c,g,i]fluorenyl‐17‐methoxycarbonyl (Tbfmoc) has been utilized as a hydrophobic probe to allow the simple, quick and highly effective isolation of a 76 residue cysteine‐containing protein (MCP‐1). The base‐labile Tbfmoc group can be removed under very mild conditions, which preserve the thiol‐con taining protein in the reduced state. Oxidative folding was then used to furnish the biologically active β‐chemokine MCP‐1.
310‐Helices, Helix Screw Sense and Screw Sense Reversal in the Dehydro‐peptide Boc‐Val‐ΔPhe‐Gly‐ΔPhe‐Val‐OMe
Authors: Tuzi Angela , Rosaria Ciajolo M. , Picone Delia , Crescenzi Orlando , Temussi Pier Andrea , Fissi Adriano , Pieroni Osvaldo 
Published: June 2010

AbstractThe pentapeptide Boc‐Val‐ΔPhe‐Gly‐ΔPhe‐Val‐OMe, containing two dehydro‐phenylalanine (ΔPhe) residues, has been synthesized and its structure investigated. In the crystalline state, the molecule adopts a right‐handed 310‐helical conformation stabilized by two intramolecular hydrogen bonds between CO of Val1 and NH of ΔPhe4, and between CO of ΔPhe2 and NH of Val5, respectively. NMR measurements are consistent with the presence of 310‐helical structures also in acetonitrile and dimethylsulphoxide solution: the distances between backbone protons estimated from NOE connectivities are in overall agreement with those observed in the solid state; the chemical shifts of the amide protons show the smaller temperature coefficients for the NHs that in solid state are involved in intramolecular hydrogen bonds. The CD spectra in acetonitrile, chloroform, methanol and dimethylsulphoxide display exciton couplets of bands corresponding to the ΔPhe electronic transition at 280nm; the sign of the bands is consistent with the presence of helical structures having a prevalent left‐handed screw sense. Addition of 1,1,1,3,3,3‐hexafluoro‐ propan‐2‐ol gives rise to the gradual appearance of a couplet of opposite sign, suggesting the helix reversal from left‐handed sense to right‐handed sense. The conformational behaviour is discussed on the basis of the specific sequence of the peptide.
Excitonic precursor states in ultrafast pump–probe spectroscopies of surface bands
Authors: Gumhalter Branko , Lazić Predrag , Došlić Nadja 
Published: June 2010

AbstractWe discuss the formation and evolution of transient coherent excitonic states induced by ultrashort laser irradiation of metal surfaces supporting the surface and image potential bands (typically the low index surfaces of Cu and Ag). These states, which evolve into the image potential states in the course of screening of primary optically excited electron–hole pair, may play the role of early intermediate states in pump–probe spectroscopies of surfaces (e.g. two‐photon‐photoemission or sum‐frequency generation) if the formation of image charge density proceeds on the time scale of the order of or longer than the pump–probe pulse duration and delay. In this regime a pump–probe experiment may yield information on the characteristics of such states rather than the states in relaxed image potential bands. Time scales of the various stages of these processes are estimated using an exactly solvable model of surface screening.
Influence of the Mn concentration on the electromechanical response d33 of Mn‐doped ZnO films
Authors: Luo J. T. , Zhu X. Y. , Chen G. , Zeng F. , Pan F. 
Published: June 2010

AbstractThe influence of Mn concentration on the electromechanical response d33 of Mn‐doped ZnO films has been investigated. It is found that d33 decreases slightly with the increase of Mn concentration until 4.8 at%, and then increases rapidly with Mn concentration increasing further. The maximum d33 value of ∼86 pC/N is obtained in Zn0.913Mn0.087O films. When the Mn concentration exceeds 8.7 at%, d33 turns to decrease. We suggest that Mn valence and ionic size in Mn‐doped ZnO films play important roles in the electromechanical response. The improved piezoelectric properties could make Mn‐doped ZnO a promising candidate for piezoelectric devices. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Neural network‐based adaptive dynamic surface control of uncertain nonlinear pure‐feedback systems
Authors: Wang Dan 
Published: June 2010

AbstractIn this paper, by incorporating the dynamic surface control technique into a neural network‐based adaptive control design framework, we have developed a backstepping‐based control design for a class of nonlinear systems in pure‐feedback form with arbitrary uncertainty. The circular design problem which may exist in pure‐feedback systems is overcome. In addition, our development is able to eliminate the problem of ‘explosion of complexity’ inherent in the existing backstepping‐based methods. A stability analysis is given, which shows that our control law can guarantee the semi‐global uniformly ultimate boundedness of the solution of the closed‐loop system, and makes the tracking error arbitrarily small. Moreover, the proposed control design scheme can also be directly applied to the strict‐feedback nonlinear systems with arbitrary uncertainty. Copyright © 2010 John Wiley & Sons, Ltd.