bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Effect of Musculoskeletal Pain on Sexual Function in Middle-aged and Elderly European Men: Results from the European Male Ageing Study
Authors: TAJAR ABDELOUAHID , O’NEILL TERENCE W. , LEE DAVID M. , O’CONNOR DARYL B. , CORONA GIOVANNI , FINN JOSEPH D. , BARTFAI GYORGY , BOONEN STEVEN , CASANUEVA FELIPE F. , FORTI GIANNI , GIWERCMAN ALEKSANDER , HAN THANG S. , HUHTANIEMI ILPO T. , KULA KRZYSZTOF , LEAN MICHAEL E.J. , PENDLETON NEIL , PUNAB MARGUS , PURANDARE NITIN , SILMAN ALAN J. , VANDERSCHUEREN DIRK , WU FREDERICK C.W. , McBETH JOHN 
Published: December 2010

Objective.To determine whether musculoskeletal pain was associated with impaired sexual function in a population sample of middle-aged and older men.Methods.The European Male Ageing Study (EMAS), a multicenter population-based study of men aged 40–79 years, was used to investigate this hypothesis. A questionnaire asked about the presence and duration of musculoskeletal pain, allowing subjects to be classified into 1 of 3 groups: those reporting chronic widespread pain (CWP), those reporting pain but not CWP (“some pain”), and those with no pain. Subjects completed a sexual function questionnaire from which 3 domains were considered: overall sexual functioning (OSF), sexual functioning-related distress (SFD), and change in sexual functioning compared to 1 year ago (CSF).Results.A total of 3206 men [mean age 60 (SD 11) yrs] had complete data on pain status. Of these, 8.7% had CWP and 50.34% had “some pain.” Pain was associated with lower OSF, and higher SFD and CSF scores. After adjustment for putative confounding factors, the associations became non-significant with OSF and CSF but persisted for SFD. Associations between pain status and some items within the sexual functioning domains, including frequency of sexual intercourse, frequency of morning erections, sexual desire, and orgasm were also significant, although these associations varied by pain status.Conclusion.Musculoskeletal pain is associated with several aspects of sexual functioning. These relationships differ depending on the extent of the pain (chronic or not) and are also largely confounded by other health-related factors, primarily depression.
Décider en temps réel : une activité située et distribuée mais aussi disloquée
Authors: Grosjean Sylvie , Robichaud Daniel 
Published: December 2010

Dans cet article, nous analysons la manière dont des opérateurs d’urgence dans un centre de répartition des appels 911 prennent des décisions en temps réel. L’objectif poursuivi consiste en la description du processus de décision en train de se faire . Il s’agit alors de rendre compte de la génération de sens dans une situation où des individus parlent, confrontent leurs points de vue, mobilisent des objets, etc. C’est à partir d’un exemple concret que nous allons souligner les modes de pensée et d’action que construisent conjointement des individus amenés à travailler ensemble et devant prendre une décision en commun. Plus spécifiquement, nous montrons en quoi une décision est à la fois locale (inscrite dans l’ici et maintenant de l’interaction) et « disloquée ». C’est-à-dire comment des « actants hétérogènes » extérieurs à la scène interactionnelle (au local de l’interaction) y sont actualisés, présentifiés, et participent à l’interaction de telle sorte que ce qui s’y passe relève tout autant d’autres lieux dans le temps et dans l’espace que de l’ici et maintenant, d’où la dis location.
Sarcoid Reactions after Chemotherapy for Hodgkin's Lymphoma
Authors: Wirk Baldeep 
Published: December 2010

Introduction There is a reported association between sarcoidosis and malignancy. This is particularly true for lymphomas and is known as the sarcoidosis-lymphoma syndrome. Case report A 49 year old Caucasian female presented with mediastinal and axillary lymphadenopathy. An excisional axillary lymph node biopsy showed classical Hodgkin's lymphoma, nodular sclerosis subtype. She received six cycles of conventional chemotherapy achieving a complete remission with no evidence of any lymphadenopathy on restaging imaging. However, one month after completion of chemotherapy, she developed new onset of progressive mediastinal lymphadenopathy. A mediastinoscopy and biopsy was performed showing noncaseating granulomata and the patient was diagnosed with a sarcoid reaction. Conclusion Sarcoidosis and sarcoid reactions must be considered in the differential diagnosis when assessing patients with persistent or enlargening masses after chemotherapy treatment for Hodgkin's lymphoma, especially since this is associated with a better prognosis. A tissue biopsy is essential prior to starting chemotherapy for presumed relapsed malignancy or persistent disease so as to avoid inappropriate treatment.
Antibodies to Mutated Citrullinated Vimentin in Rheumatoid Arthritis: Diagnostic Value, Association with Radiological Damage and Axial Skeleton Affection
Authors: Mansour Howaida E. , Metwaly Khaled M. , Hassan Iman A. , Elshamy Hebat-Allah A. , Elbeblawy Moataz M.S. 
Published: December 2010

Background Early definitive diagnosis and effective treatment are mandatory in rheumatoid arthritis (RA) as it can halt the disease progression and subsequent joints destruction. Objective To investigate the diagnostic and prognostic value of anti-mutated citrullinated vimentin (anti-MCV) and its correlation with disease activity, peripheral and axial skeleton affection in RA patients. Patients and Methods A total of 123 patients with different rheumatic diseases were enrolled in a prospective-two year study at Ain Shams University hospital: 64 patients with RA and 59 patients with other rheumatic diseases as controls. RA patients were fulfilling the traditional and the new ACR/EULAR diagnostic criteria for RA. They have been followed up for two years. At baseline, all RA patients were subjected to: Clinical assessment of disease activity by taking full histories, general and local examination, measurement of 28 joint count of tender and swollen joints with calculation of disease activity score (DAS-28) for each patient. Complete blood count, erythrocytes sedimentation rate, C-reactive protein and rheumatoid factor titers were performed. Anti-MCV IgG immunoglobulins’ assay was performed at the study endpoint by ELISA. RA patients were then classified into; anti-MCV positive and anti-MCV negative groups for statistical comparison. Plain X-ray was performed on the peripheral joints and scored by the Simple Erosion Narrowing score (SEN-score). Magnetic Resonance Imaging (MRI) scans were carried out to 22 RA patients on cervical and lumbosacral regions. Results Anti-MCV antibodies were found to be of high sensitivity (79.6%) and specificity (96.6%) in diagnosing RA. The area under the curve was 0.893 at 95% confidence interval (CI), confers an odds ratio of 23.5. Anti-MCV positive RA patients had significantly higher DAS-28 and SEN-scores than anti-MCV negative patients; who were found to have more benign disease with lower incidence of erosions ( P < 0.05). MRI scans revealed that; 17/22 (77%) had cervical joints involvement while, 8 (36%) had lumbo-sacral joint lesions ( P < 0.05), both were correlated significantly with aggressive peripheral joint disease. Conclusion Anti-MCV antibodies are promising diagnostic and prognostic marker in RA, with high sensitivity and specificity. They may identify a subset of RA patients with aggressive early erosive disease. The axial skeleton—especially the cervical spine—could be affected in RA and this was correlated with aggressive peripheral joints’ disease. MRI scanning is a sensitive method for detecting axial skeleton involvement in RA, in attempt for better disease control and outcomes.
Pattern of Young and Old Onset Rheumatoid Arthritis (YORA and EORA) among a Group of Egyptian Patients with Rheumatoid Arthritis
Authors: El-Labban Abdou S. , Omar Hanaa A.S. Abo , El-Shereif Rawhya R. , Ali Fatma , El-Mansoury Tarek M. 
Published: December 2010

Objective Rheumatoid arthritis (RA) differs depending on the age of disease onset. The differences between EORA and YORA are important because they have clinical and therapeutic implications. Method 1185 patients were ranked after classification according to age at onset of the disease into YORA I (16–40 years), YORA II (41–60 years) and EORA >60 years. All patients groups were compared, based on disease duration, disease activity, severity parameters and drug history. Results YORA I included 298 patients, 28.85% were males, with mean age of 29.4 ± 6 years and disease duration 4 ± 3.3 y, YORA II included 539 patients, 33.77% males, age 49.7 ± 6.1 y and disease duration 6.5 ± 5.6 y. EORA included 348 RA patients 40.5% males, age 67.1 ± 6.6 y, disease duration 9.95 ± 7.2 y. Activity was increased in EORA compared to YORA I and YORA II, while severity decreased in EORA. ESR, CRP and degree of anemia were higher in EORA. RF titer was higher in YORA. Small joints of the hands and feet were more involved in YORA, while, large joints in EORA. Rheumatoid nodules were increased in YORA I than EORA P = 0.04. Polymyalgia rheumatica was exclusively present in EORA group 25 patients 7.2%. Methotrexate was used in both YORA and EORA, with a higher mean of dosage in YORA than EORA. Multiple DMARDs in EORA was 57.9%, and biologics in 0.8% was which was significantly lower compared with YORA I, 86.3% and 1.7%, with P = 0.001. Conclusion EORA has more active and less disabling and affects more males than YORA. The use of biologic therapy and combination DMARD therapy was less in EORA.
Valvular Aortic Stenosis: A Proteomic Insight
Authors: Gil-Dones Felix , Martin-Rojas Tatiana , Lopez-Almodovar Luis F. , De La Cuesta Fernando , Darde Veronica M. , Alvarez-Llamas Gloria , Juarez-Tosina Rocio , Barroso Gemma , Vivanco Fernando , Padial Luis R. , Barderas Maria G. 
Published: December 2010

Calcified aortic valve disease is a slowly progressive disorder that ranges from mild valve thickening with no obstruction of blood flow, known as aortic sclerosis, to severe calcification with impaired leaflet motion or aortic stenosis. In the present work we describe a rapid, reproducible and effective method to carry out proteomic analysis of stenotic human valves by conventional 2-DE and 2D-DIGE, minimizing the interference due to high calcium concentrations. Furthermore, the protocol permits the aortic stenosis proteome to be analysed, advancing our knowledge in this area. Until recently, aortic stenosis (AS) was considered a passive process secondary to calcium deposition in the aortic valves. However, it has recently been highlighted that the risk factors associated with the development of calcified AS in the elderly are similar to those of coronary artery disease. Furthermore, degenerative AS shares histological characteristics with atherosclerotic plaques, leading to the suggestion that calcified aortic valve disease is a chronic inflammatory process similar to atherosclerosis. Nevertheless, certain data does not fit with this theory making it necessary to further study this pathology. The aim of this study is to develop an effective protein extraction protocol for aortic stenosis valves such that proteomic analyses can be performed on these structures. In the present work we have defined a rapid, reproducible and effective method to extract proteins and that is compatible with 2-DE, 2D-DIGE and MS techniques. Defining the protein profile of this tissue is an important and challenging task that will help to understand the mechanisms of physiological/pathological processes in aortic stenosis valves.
The Liver and Right Atrium—Hepatic Cyst as a Cause of Arrhythmia
Authors: Ker James 
Published: December 2010

Simple hepatic cysts are a relatively common radiological finding. These cysts may be classified as parasitic and non-parasitic. They are usually asymptomatic, but may cause symptoms due to local compression. These compressive complications include: portal hypertension, edema due to caval compression, extrinsic gastric compression and duodenal compression with obstruction. However, no reports in the literature exist describing atrial compression by hepatic cysts. In this case report a simple hepatic cyst causing slight right atrial compression is described. This slight compression is the cause of atrial premature beats. It is proposed that simple hepatic cysts may be the cause of atrial premature beats.
Similar Results in Children with Asthma for Steady State Pharmacokinetic Parameters of Ciclesonide Inhaled with or without Spacer
Authors: Boss H. , Minic P. , Nave R. 
Published: December 2010

Background Ciclesonide is an inhaled corticosteroid administered by a metered dose inhaler (MDI) to treat bronchial asthma. After inhalation, the inactive ciclesonide is converted by esterases in the airways to active metabolite desisobutyryl-ciclesonide (des-CIC). Aim To compare the pharmacokinetic (PK) parameters of des-CIC in children after administration of therapeutic dose of ciclesonide with and without spacer (AeroChamber Plus™). Methods Open-label, 3 period, cross over, repeated dose, PK study in 37 children with mild to moderate stable asthma (age: 6–11 y; body weight: 20–53 kg). During each 7-day treatment period, ciclesonide was inhaled once in the morning: A) 160 μg MDI with spacer, B) 80 μg MDI with spacer, and C) 160 μg MDI without spacer. Serum PK parameters of ciclesonide and des-CIC were determined on Day 7 of each period. The primary PK parameters were the AUCτ and Cmax for des-CIC. Results Inhaling ciclesonide with spacer led to a dose proportional systemic exposure (AUCτ) of des-CIC (0.316 μg*h/L for 80 μg and 0.663 μg*h/L for 160 μg). The dose-normalized systemic exposure for des-CIC (based on AUCτ) was 27% higher after inhalation of ciclesonide 80 μg or 160 μg with spacer than without spacer; the corresponding Cmax values for des-CIC were, respectively, 63% and 55% higher with spacer. No clinically relevant abnormalities or adverse drug reactions were observed. Conclusions Inhalation of therapeutic ciclesonide dose with spacer led to a slight increase in the systemic exposure of des-CIC, which does not warrant dose adjustment.
The Nucleobase‐Cation‐Symport‐1 Family of Membrane Transport Proteins
Authors: Weyand Simone , Ma Pikyee , Saidijam Massoud , Baldwin Jocelyn , Beckstein Oliver , Jackson Scott , Suzuki Shun'ichi , Patching Simon G , Shimamura Tatsuro , Sansom Mark SP , Iwata So , Cameron Alexander D , Baldwin Stephen A , Henderson Peter JF 
Published: December 2010

AbstractThe evolutionary relationships of membrane transport proteins of the nucleobase‐cation‐symport (NCS‐1) family from bacteria, fungi, and plants are described. The reported substrates of the NCS‐1 family include nucleobases, hydantoins, and vitamins. Secondary active transport of substrate accompanied by a sodium ion, or possibly a proton, is the usual mechanism of energization. A strategy for the amplified expression, purification, and activity assays of bacterial members of the NCS‐1 family is described. Conditions are given for the production of diffracting crystals of one member, the Na+‐coupled transporter for aromatic hydantoins, ‘Mhp1’, fromMicrobacterium liquefaciens. The 3D structures of three forms of the Mhp1 protein are discussed in terms of one open‐outward, one substrate‐occluded, and one open‐inward conformation contributing to a molecular dynamics simulation of the alternating‐access model of membrane transport. The unexpected similarity of the protein fold of Mhp1 to those of transport proteins, hitherto thought to be from different evolutionary families, is discussed.
Peptidyl‐α‐Hydroxyglycine α‐Amidating Lyase (PAL)
Authors: Chufán Eduardo E , Eipper Betty , Mains Richard , Amzel Mario L 
Published: December 2010

AbstractMany peptide hormones and neuropeptides require an amidated carboxy terminal for full biological activity. This reaction is carried out in two steps: hydroxylation of a C‐terminal glycine and cleavage of the C N bond with the release of glyoxylate. The enzyme peptidyl‐α‐hydroxyglycine α‐amidating lyase (PAL; EC 4.3.2.5) catalyzes the second step of this reaction,N‐dealkylation of the peptidyl‐α‐hydroxyglycine to generate the α‐amidated peptide and glyoxylate. The X‐ray crystal structure of the PAL catalytic core (PALcc), alone and in complex with the nonpeptidic substrate α‐hydroxyhippuric acid, shows that PAL folds as a six‐bladed β‐propeller. The active site is formed by a Zn2+ion coordinated by three histidine residues; the substrate binds to this site with its α‐hydroxyl group coordinated to the Zn2+ion. A tyrosine residue (Tyr654) at the active site acts as the catalytic base for hydroxyl deprotonation, an unusual role for tyrosine residue. In the reaction mechanism, proposed on the basis of the structure and validated by biochemical analysis of site‐directed PALcc mutants, the substrate α‐hydroxyl that coordinates the catalytic Zn2+directly is deprotonated by Tyr654. The generation of the required tyrosinate is aided by an arginine residue that, together with the Zn2+, lowers the pKaof the catalytic tyrosine.
Mo–Se‐Containing Nicotinate Dehydrogenase
Authors: Dobbek Holger , Pierik Antonio J 
Published: December 2010

AbstractSe Mo‐containing nicotinate dehydrogenase (NDH) of the anaerobic soil bacteriumEubacterium barkericatalyzes the hydroxylation of nicotinate with water and NADP+to 6‐hydroxynicotinate, one proton, and NADPH. This reaction is the first step in the degradation of nicotinate to pyruvate and propionate in a unique fermentation pathway. NDH is constituted by four different subunits and contains a Mo‐bound pyranopterin cofactor linked to cytosine dinucleotide, two different [2Fe2S] centers, and flavin adenine dinucleotide to facilitate the transfer of a hydride ion on NADP+. NDH is a member of the family of molybdenum hydroxylases and shares several distinctive features with other members of this enzyme family, such as xanthine oxidoreductase, aldehyde oxidase, and the Mo‐containing carbon monoxide dehydrogenase. The reactivity of NDH critically depends on the presence of selenium in the active site, where it is bound as a labile nonselenocysteinyl selenium ligand to molybdenum. The role of the selenium ligand is likely to accelerate the formal hydride transfer from the substrate into the Mo Se bond, displaying a novel reactivity of selenium in nature.
d
‐ and
f
‐
B
lock Transition Metal Complexes with Bulky Allyl Ligands
Authors: Chmely Stephen C. , Hanusa Timothy P. 
Published: December 2010

Abstract

The allyl anion (C
3
H
5
−
) is often found in combination with other ligands in organometallic complexes. Its compact size, however, means that compounds in which it is the only or principal type of ligand are frequently coordinatively unsaturated and prone to decomposition. Sterically bulky substituents (e.g.,—SiMe
3
) placed on the allyl group will increase its effective size, often enhancing the thermal, and in some cases oxidative, stability of the associated metal complexes. Compounds of the d‐ and f‐block metals containing bulky allyl ligands display a wide range of monomeric, oligomeric, and polymeric structures. Some of these complexes have no counterparts with unsubstituted allyls, and others are active polymerization catalysts. Research in this area has markedly expanded the range of metals, structural types, and associated reactions that can be incorporated into metal–allyl chemistry.
Medicinal Inorganic Chemistry: Correcting Essential Metal‐Ion Deficiencies
Authors: Thompson Katherine H. 
Published: December 2010

AbstractEssential metal ions are those for which there is a known requirement for good health in the body. When dietary intake does not, or cannot, maintain adequate tissue stores of an essential trace or ultratrace metal ion, metal‐ion supplementation may be necessary. Simple salts of metal ions are often poorly tolerated, not well absorbed, or too rapidly excreted (or all three of these); therefore, appropriate ligand binding of the particular metal ion can significantly improve the success of supplementation efforts. The most common deficiency disorder, iron‐deficiency anemia, is now treated with a number of iron complexes, e.g., Ferrochel™ and ferrous gluconate, that are superior to simple iron salts such as ferrous sulfate; others, such as ferric maltol, are being proposed as useful substitutes. Marginal deficiencies of trace and ultratrace elements, such as zinc, copper, manganese, and chromium, are purported to have long‐term negative health effects, especially in aging or ill populations. Defining marginal deficiency is difficult, owing to lack of reliable and accurate biomarkers for the determination of trace element status.
Optical Tweezers
Authors: Greulich Karl Otto 
Published: December 2010

AbstractInfrared lasers focused with high numerical aperture into a microscope are used to exert and measure forces in the piconewton range. Simple light pressure and the more complex ‘gradient forces’ are exploited. Unlike any other micromanipulation tool, optical tweezers can, for example, be used to work in the interior of unopened living cells to support cell fusion, to stimulate specificity in the interaction of immune cells and to simulate heart infarction. Here, a variant of optical tweezers, erythrocyte‐mediated force application is particularly helpful. Other applications are in humanin vitrofertilisation, where hundreds of children owe their existence to optical tweezers, and blood pressure studies. Studies on infection of a single cell by a virus or bacterium and on the organisation, of one of the fundamental processes of life – the generation of motion (by the centrosome) – complement the wide field of applications of optical tweezers in the life sciences.Key Concepts:Light is focused with very high aperture into a microscope so that light pressure and gradient forces can be used to hold, manipulate and measure forces of microscopic objects.Optical tweezers can be added to most standard microscopes with only little effort.Infrared light is used, which has a large penetration depth in biological tissue. Thus, one can work in the interior of unopened objects.With red blood cells as handle, forces of optical tweezers can be fully exploited (erythrocyte‐mediated force application, EMFA).Optical tweezers can be used to establish contact between microsopic objects as well as to move particles and organelles in the interior of closed objects.The force transduction is gentle and thus also suitable for fragile targets.
tRNABiogenesis
Authors: Jackman Jane E 
Published: December 2010

AbstractDuring transferribonucleic acid(tRNA) biogenesis, tRNA molecules undergo extensive processing before they can fulfill their essential role as the adapter molecule in translation, bringing amino acids into the ribosome for protein synthesis. Many components of the tRNA processing machinery have been identified in a variety of organisms, and a comparison of these shows many common features. However, species‐specific features have also been identified, and these present interesting examples of alternative evolutionary pathways and suggest additional interactions between tRNA processing machinery and other cellular processes. An increasing number of mechanisms have been identified that serve to safeguard the tRNA population, either by repair or removal of damaged tRNA species. A picture emerges of a tightly controlled and complex process required for tRNA biogenesis.Key Concepts:tRNA molecules are heavily processed before their use in translation.Many aspects of tRNA splicing are conserved in multiple domains of life, but there are significant species‐specific differences.tRNA modification enzymes exhibit different mechanisms for recognition of specific tRNA species to be modified.Multiple quality control pathways exist that serve to repair and protect the cellular tRNA pool.