bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design of Artificial C-Peptides as Potential Anti-HIV-1 Inhibitors Based
on 6-HB Formation Mechanism
Authors: Luo Hui , Zhao Yan , Ma Yuheng , Liang Guodong , Ga Lu , Meng Zhao 
Published: June 2024

Background:
The six-helix bundle (6-HB) is a core structure formed during the membrane
fusion process of viruses with the Class I envelope proteins. Peptide inhibitors, including
the marketed Enfuvirtide, blocking the membrane fusion to exert inhibitory activity were designed
based on the heptads repeat interactions in 6-HB. However, the drawbacks of Enfuvirtide, such as
drug resistance and short half-life in vivo, have been confirmed in clinical applications. Therefore,
novel design strategies are pivotal in the development of next-generation peptide-based fusion inhibitors.

Objective:
The de novo design of α-helical peptides against MERS-CoV and IAVs has successfully
expedited the development of fusion inhibitors. The reported sequences were completely nonhomologous
with natural peptides, which can provide some inspirations for the antiviral design
against other pathogenic viruses with class I fusion proteins. Here, we design a series of artificial
C-peptides based on the similar mechanism of 6-HB formation and general rules of heptads repeat
interaction.

Methods:
The inhibitory activity of peptides against HIV-1 was assessed by HIV-1 Env-mediated
cell-cell fusion assays. Interaction between artificial C-peptides and target peptides was evaluated
by circular dichroism, polyacrylamide gel electrophoresis, size-exclusion chromatography, and
sedimentation velocity analysis. Molecular docking studies were performed by using Schrödinger
molecular modelling software.

Results:
The best-performing artificial C-peptide, 1SR, was highly active against HIV-1 env-mediated
cell-cell fusion. 1SR binds to the gp41 NHR region, assembling polymer to prevent endogenous
6-HB formation.

Conclusion:
We have found an artificial C-lipopeptide lead compound with inhibitory activity
against HIV-1. Also, this paper enriched both N- and C-teminal heptads repeat interaction rules in
6-HB and provided an effective idea for next-generation peptide-based fusion inhibitors against
HIV-1.
Structural Properties of Rat Intestinal Fatty Acid-Binding Protein with its
Dynamics: Insights into Intrinsic Disorder
Authors: Balli Oyku Irem , Caglayan Sule Irem , Uverksy Vladimir N. , Coskuner-Weber Orkid 
Published: June 2024

Background:
The rat intestinal fatty acid-binding protein (I-FABP) is expressed in the
small intestine and is involved in the absorption and transport of dietary fatty acids. It is used as a
marker for intestinal injury and is associated with various gastrointestinal disorders. I-FABP has
been studied extensively using conventional experimental and computational techniques. However,
the detection of intrinsically disordered regions requires the application of special sampling
molecular dynamics simulations along with certain bioinformatics because conventional computational
and experimental studies face challenges in identifying the features of intrinsic disorder.

Methods:
Replica exchange molecular dynamics simulations were conducted along with bioinformatics
studies to gain deeper insights into the structural properties of I-FABP. Specifically, the Cα
and Hα chemical shift values werecalculated, and the findings were compared to the experiments.
Furthermore, secondary and tertiary structure properties were also calculated, and the protein was
clustered using k-means clustering. The end-to-end distance and radius of gyration values were reported
for the protein in an aqueous solution medium. In addition, its disorder tendency was
studied using various bioinformatics tools.

Results and Conclusion:
It was reported that I-FABP is a flexible protein with regions that demonstrate
intrinsic disorder characteristics. This flexibility and intrinsic disorder characteristics of IFABP
may be related to its nature in ligand binding processes.
Current Insights in Murine Models for Breast Cancer: Present, Past and Future
Authors: Jain Mansi , Goel Anjana 
Published: June 2024

:
Breast cancer is an intricate disease that is increasing at a fast pace, and numerous heterogeneities
within it further make it difficult to investigate. We have always used animal models to understand cancer
pathology and create an in vivo microenvironment that closely resembles human cancer. They are considered
an indispensable part of any clinical investigation regarding cancer. Animal models have a high potency in
identifying the relevant biomarkers and genetic pathways involved in the course of disease prognosis. Researchers
have previously explored a variety of organisms, including Drosophila melanogaster, zebrafish, and
guinea pigs, to analyse breast cancer, but murine models have proven the most comprehensive due to their homologous
nature with human chromosomes, easy availability, simple gene editing, and high adaptability. The
available models have their pros and cons, and it depends on the researcher to select the one most relevant to
their research question. Chemically induced models are cost-effective and simple to create. Transplantation
models such as allografts and xenografts can mimic the human breast cancer environment reliably. Genetically
engineered mouse models (GEMMs) help to underpin the genetic alterations involved and test novel immunotherapies.
Virus-mediated models and gene knockout models have also provided new findings regarding
breast cancer progression and metastasis. These mouse models have also enabled the visualization of breast
cancer metastases. It is also imperative to consider the cost-effectiveness of these models. Despite loopholes,
mouse models have evolved and are required for disease analysis.
Synthesis and Biological Evaluation of Gastroprotective Polymer Conjugates of
Aceclofenac
Authors: Mishra Monika , Chawla Viney , Chawla Pooja A. 
Published: June 2024

:
Nonsteroidal anti-inflammatory drugs account for a sizeable fraction of the drugs
prescribed worldwide. If consumed for a long time, they can cause ulcers and lifethreatening
side effects. Since the acidic group present in NSAIDs is mainly responsible for
these side effects, the scientists try to synthesize polymer drug conjugates that avoid these
side effects but still retain the potency of the parent drug. Macromolecular drug conjugates
of aceclofenac were prepared by employing pectin, β cyclodextrin, deacetylated chitin (chitosan)
and albumin (egg and bovine serum). The prepared conjugates were characterized
and tested for their anti-inflammatory, antinociceptive and antiulcerogenic activity. Further
experimentation was undertaken to analyze the behavior of compounds and their stability in
hydrolytic conditions. Test compound A1, a pectin conjugate of aceclofenac, exhibited significant
antinociceptive and anti-inflammatory activity with a significant decrease in ulcer index (4.45±0.24) as
against aceclofenac (8.61±0.40) or vehicle (12.39±0.44) treated group. A novel and safer polymer drug conjugate
of aceclofenac has been synthesized and evaluated.
Harnessing Bioprinting Technologies for Diabetic Wound Regeneration
Authors: Dhankhar Sanchit , Garg Nitika , Chauhan Samrat , Saini Monika , Singh Thakur Gurjeet 
Published: June 2024

Abstract:
A chronic metabolic condition, diabetes mellitus (DM), is becoming more common
all over the globe. Diabetic complications include diabetic foot ulcers (DFUs). Between fifteen
and twenty-five percent of people with diabetes will experience DFU at some point in
their lives. Prolonged hospital stays and amputations are common outcomes of DFUs due to
the absence of targeted therapy and appropriate wound dressings. Specialized DFU wound
care is expected to be in high demand due to the anticipated increase in the prevalence of DM.
Therefore, there is a strong need to enhance and create more effective wound dressings and
therapies that are unique to DFU. Bioengineered tissues, individualised prostheses, and implants
are just a few examples of how 3D bioprinting has revolutionised healthcare in the past
decade. This review delves into the difficulties of wound management and explores how 3D
bioprinting could improve existing treatment approaches and biomanufacturing composite 3D
human skin substitutes as an alternative to skin grafting. To alleviate the healthcare burden
caused by the rising incidence of DM, it will be crucial to co-develop 3D bioprinting technologies
with new therapeutic techniques to address the unique pathophysiological problems of
DFU in the future.
Evaluation of Novel HLM Peptide Activity and Toxicity against Planktonic and Biofilm Bacteria: Comparison to Standard Antibiotics
Authors: Masadeh Majed M. , Alshogran Haneen , Alsaggar Mohammad , Sabi Salsabeel H. , Al Momany Enaam M. , Masadeh Majd M. , Alrabadi Nasr , Alzoubi Karem H. 
Published: June 2024

Background:
Antibiotic resistance is one of the main concerns of public health, and the
whole world is trying to overcome such a challenge by finding novel therapeutic modalities and
approaches. This study has applied the sequence hybridization approach to the original sequence
of two cathelicidin natural parent peptides (BMAP-28 and LL-37) to design a novel HLM peptide
with broad antimicrobial activity.

Methods:
The physicochemical characteristics of the newly designed peptide were determined. As
well, the new peptide’s antimicrobial activity (Minimum Inhibitory Concentration (MIC), Minimum
Bacterial Eradication Concentration (MBEC), and antibiofilm activity) was tested on two
control (Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922) and two resistant
(Methicillin-resistant Staphylococcus aureus (MRSA) ATCC BAA41, New Delhi metallo-beta-
lactamase-1 Escherichia coli ATCC BAA-2452) bacterial strains. Furthermore, synergistic
studies have been applied to HLM-hybridized peptides with five conventional antibiotics by
checkerboard assays. Also, the toxicity of HLM-hybridized peptide was studied on Vero cell lines
to obtain the IC50 value. Besides the percentage of hemolysis action, the peptide was tested in
freshly heparinized blood.

Results:
The MIC values for the HLM peptide were obtained as 20, 10, 20, and 20 μM, respectively.
Also, the results showed no hemolysis action, with low to slightly moderate toxicity action
against mammalian cells, with an IC50 value of 10.06. The Biomatik corporate labs, where HLM
was manufactured, determined the stability results of the product by Mass Spectrophotometry (MS)
and High-performance Liquid Chromatography (HPLC) methods. The HLM-hybridized peptide
exhibited a range of synergistic to additive antimicrobial activities upon combination with
five commercially available different antibiotics. It has demonstrated the biofilm-killing effects in
the same concentration required to eradicate the control strains.

Conclusion:
The results indicated that HLM-hybridized peptide displayed a broad-spectrum activity
toward different bacterial strains in planktonic and biofilm forms. It showed synergistic or additive
antimicrobial activity upon combining with commercially available different antibiotics.
Downregulation of Tnf-α and Cat Expression in a Wistar Rat Diabetic Model during Diabetes Onset
Authors: Arcia Catherine Giovanna Costas , Encinas Jessica Freitas Araujo , Raimundo Joyce Regina Santos , de Gois Katharyna Cardoso , da Costa Aguiar Alves Beatriz , Perez Matheus Moreira , Gascon Thais Moura , Fonseca Fernando Luiz Affonso , da Veiga Glaucia Luciano 
Published: June 2024

Introduction:
Diabetes Mellitus (DM) is a metabolic disorder characterized by persistent
hyperglycemia and/or insulin resistance. If left uncontrolled, it can lead to a combination of
cardiac and renal alterations known as cardiorenal syndrome. Additionally, oxidative stress and
inflammation contribute to tissue damage, thereby reducing the life expectancy of individuals
with diabetes.

Aim:
The aim of this study was to identify early molecular markers associated with cardiorenal
syndrome, oxidative stress, and inflammation, and to investigate their correlation with the duration
of exposure to DM.

Methods:
An experimental DM model was employed using Wistar rats. The rats were divided
into four groups: diabetic rats at 7 days (DM7), diabetic rats at 30 days (DM30), control sham at
7 days (CS7), and control sham at 30 days (CS30). Blood and brain tissue from the brainstem region
were collected at 7 and 30 days after confirming DM induction. Gene expression analysis of
Bnp, Anp, Cat, Gpx, Sod, Tnf-α, and Il-6 was performed.

Results:
The analysis revealed lower expression values of Cat in the brainstem tissue of the
DM7 group compared to the NDS7 group. Moreover, diabetic animals exhibited statistically
lower levels of Tnf-α in their peripheral blood compared to the control animals.

Conclusion:
This study concluded that DM alters the oxidative balance in the brainstem after 7
days of DM induction, resulting in lower Cat expression levels. Although some genes did not
show statistical differences after 30 days of DM induction, other genes exhibited no expression
values, indicating possible gene silencing. The study identified an imbalance in the studied
pathways and concluded that the organism undergoes a compensatory state in response to the initial
metabolic alterations caused by DM.
Cardiology and Neurophysiological Stimulation of Internet Gaming
Disorders: A Systematic Review
Authors: Selvam Immaculate Joy 
Published: June 2024

Background:
Internet Gaming Disorder (IGD) is recognized as a mental health condition associated with excessive video gaming, leading to functional impairments. The inclusion of
IGD in the DSM-5 has underscored the importance of comprehensively understanding its physiological and psychological effects.

Objective:
This systematic review aims to analyze and synthesize existing literature on the cardiophysiological and neurophysiological activities of individuals diagnosed with IGD, with a focus on identifying patterns, trends, and implications for clinical practice and future research.

Methods:
A systematic search was conducted in PubMed and Scopus databases to identify relevant studies published up to 2023. The search strategy included terms related to IGD, cardiophysiology, neurophysiology, and relevant measurement techniques. Inclusion criteria encompassed peer-reviewed research articles and clinical trials examining cardiophysiological (e.g.,
heart rate variability, blood pressure) and neurophysiological (e.g., brain imaging, electroencephalography) parameters in individuals with IGD. Exclusion criteria were applied to ensure
methodological rigor and relevance to the research question.

Results:
The initial search yielded 1320 papers related to IGD, of which twenty studies met the
eligibility criteria and were included in the review. Data extraction and synthesis focused on key
cardiophysiological and neurophysiological outcomes observed in individuals with IGD compared to healthy controls. Findings revealed decreased Heart Rate Variability (HRV), increased
sympathetic activity, and executive control deficits in IGD individuals based on Electrocardiogram (ECG) recordings and cognitive assessments. Neuroimaging studies demonstrated heightened brain activation in the lateral and prefrontal cortex, altered reward processing, and impulse
control mechanisms among IGD subjects. Gender-specific differences were noted, with males
exhibiting distinct thalamic activation striatum and decreased Regional Homogeneity (ReHo) in
the right Posterior Cingulate (rPCC) compared to females.

Discussion:
The synthesized evidence indicates a complex interplay between excessive gaming
and cardiophysiological/neurophysiological changes, highlighting the need for multidimensional
assessments in diagnosing and managing IGD. Implications for clinical practice include early
detection using ECG, EEG, and advanced neuroimaging techniques, as well as personalized interventions tailored to individual characteristics and gender-specific differences.

Conclusion:
This systematic review provides a comprehensive overview of the cardiophysiological and neurophysiological activities associated with Internet Gaming Disorder. The findings
underscore the need for further research to elucidate underlying mechanisms, develop standardized diagnostic protocols, and optimize targeted interventions for individuals with IGD.
Synchronized Assessment of Lobeglitazone Sulfate and Metformin Hydrochloride in Tablet by Robust, High-performance Thin-layer Chromatographic Method
Authors: Sen Dhanya B. , Baldha Krunal , Sen Ashim K. , Maheshwari Rajesh A. , Zanwar Aarti S. , K. P. Greeshma , Pradhan Prasanna K. 
Published: June 2024

Background:
A combination of fixed-doses containing 0.5 mg lobeglitazone sulfate
and 500 mg metformin hydrochloride has demonstrated efficacy in enhancing glycemic control in
diabetes.

Aims:
The projected work aimed to establish and validate a high-performance thin-layer chromatographic
methodology for the quantification of both drugs in tablet formulations.

Objectives:
The task involves creating and validating a method in accordance with ICH guidelines
to quantify two particular drugs in tablet formulations accurately.

Methods:
The high-performance thin-layer chromatographic analysis utilized aluminum plates layered
with silica gel 60F254, and the solvent system consisted of acetonitrile, 1 M ammonium acetate
(methanol), toluene, and triethyl amine (1.5:2.5:4:0.2 v/v/v/v), followed by densitometric scanning
at 237 nm.

Results:
The methodology exhibited linearity in the range of 100-1500 ng/band for lobeglitazone
sulfate and 1000-15000 ng/band for metformin hydrochloride, with correlation coefficients of
0.9991 and 0.9992, correspondingly. Exceptional sensitivity was observed, with detection limits
of 8.17 ng/band for lobeglitazone sulfate and 271.34 ng/band for metformin hydrochloride, along
with quantification limits of 24.75 ng/band for lobeglitazone sulfate and 822.24 ng/band for metformin
hydrochloride. The method demonstrated precision (% relative standard deviation of peak
area <2) and accuracy (recovery between 96 and 103%).

Conclusion:
The suggested methodology is fit for the concurrent quantification of both drugs in
tablet formulations, making it applicable for routine quality control assessments in laboratories.
Treatment of Tislelizumab-Induced Toxic Epidermal Necrolysis and Agranulocytosis: A Case Report and Literature Review
Authors: Zhou Yanshi , Xue Honghao , Lu Chenghua , Zhang Yemin , Wu Qingyuan , Zhang Jun , Xie Shiyun , Xu Xiangqian , Guo Xiaoyan 
Published: June 2024

Background:
Non-small Cell Lung Cancer (NSCLC) makes up about 85% of lung
cancer cases, mainly adenocarcinoma and squamous cell carcinoma. Recently, PD-1 inhibitors
have become crucial in NSCLC treatment, significantly enhancing survival for some. However,
side effects, like skin reactions and hematotoxicity, limit their use, with drug-induced TEN and
immunotherapy-induced agranulocytosis as severe adverse effects.

Case Presentation:
Herein, we have reported the case of a 75-year-old male diagnosed with
metastatic Lung Squamous cell Carcinoma (LUSC) in the left lung. He received first-line treatment
with one cycle of tislelizumab in combination with nab-paclitaxel and carboplatin, after
which he developed Toxic Epidermal Necrolysis (TEN) and granulocytopenia. To address these
two serious immune-related Adverse Events (irAEs), the patient was administered methylprednisolone
in combination with gamma globulin for TEN and dexamethasone in combination with
G-CSF for agranulocytosis. Antibiotics were also administered according to the patient’s medication
regimen. After treatment, the patient recovered and was discharged from the hospital. It
was also noted that the lung tumor condition improved.

Conclusion:
Effective management of severe immune-related side effects from tislelizumab, including
TEN and agranulocytosis, can be partly achieved through steroids, gamma globulin, GCSF,
and antibiotics. This strategy not only alleviates these adverse effects, but also potentially
improves tumor conditions, highlighting the crucial role of vigilant monitoring and management
in immunotherapy.
Comparación de los niveles de inteligencia emocional y creatividad en niños escolarizados y educados en casa homeschoolers
Authors: Melo Erika 
Published: June 2024

Introducción: La demanda del mundo actual requiere individuos que sean capaces de adaptarse, relacionarse y encontrar soluciones precisas para las diversas circunstancias sin importar su origen o el estilo de aprendizaje al que hayan tenido acceso. Por esto, la inteligencia emocional y la creatividad desempeñan un papel relevante en el éxito de los individuos. Objetivo: Comparar el nivel de desarrollo de la inteligencia emocional y de la creatividad en niños y niñas con edades entre 8 y 10 años de dos grupos (escolarizados: 35, y homeschoolers: 9). Metodología: Se analizan los resultados obtenidos por los dos grupos de niños aplicando la escala TMMS-24 y el test de pensamiento creativo de Torrance, adaptado para el idioma español. Resultados: Se observa que los niños escolarizados obtienen mayor nivel de creatividad y los niños homeschoolers mayor nivel de inteligencia emocional. Conclusiones: Tanto los niños escolarizados como los homeschoolers tienen posibilidades de desarrollar la inteligencia emocional y la creatividad en los ambientes educativos. Es necesario que los adultos que acompañan los procesos promuevan más conscientemente actividades y herramientas direccionadas a estas áreas del desarrollo infantil.
Study on the Construction Path of Master Studios for Vocational Employment and Entrepreneurship Guidance
Authors: Shi Xia , Li Hongyu 
Published: June 2024

In the process of cultivating highly skilled technical talents in higher vocational colleges, employment and entrepreneurship guidance plays a crucial role. However, there are still problems such as insufficient resources and unsatisfactory guidance effects in the current vocational colleges’ employment and entrepreneurship guidance. To solve these problems, this study proposes the construction of master studios for employment and entrepreneurship guidance and discusses their construction paths in detail. Through literature analysis, questionnaires, and interviews, this paper analyzes the current status and existing problems of employment and entrepreneurship guidance in vocational colleges, explores the concept, role, and necessity, and feasibility of master studios. The research results show that master studios can significantly improve students’ employment and entrepreneurship abilities and promote the teaching quality of vocational colleges. Based on this, this paper proposes a systematic construction path for master studios, including goal setting, organizational structure design, operation mode, resource allocation and guarantee, and the cultivation and development of the master team. Finally, by analyzing successful cases, the experience and insights of master studio construction are summarized to provide theoretical basis and practical guidance for the construction of master studios for employment and entrepreneurship guidance in vocational colleges.