bluuebunny/crossref_metadata_2025_split huggingface dataset:
Learning curve and pitfalls of a laparoscopic score to describe peritoneal carcinosis in advanced ovarian cancer
Authors: FAGOTTI ANNA , VIZZIELLI GIUSEPPE , COSTANTINI BARBARA , LECCA ANTONELLA , GALLOTTA VALERIO , GAGLIARDI MARIA LUCIA , SCAMBIA GIOVANNI , FANFANI FRANCESCO 
Published: June 2011

AbstractObjective. To prospectively estimate the agreement between a fellow in training in gynecologic oncology and a senior surgeon performing a laparoscopic score to describe peritoneal carcinosis diffusion in patients with advanced ovarian cancer. Design: Single‐institutional non‐inferiority trial. Setting. University hospital tertiary care center. Population. Ninety consecutive patients with primary advanced ovarian cancer. Methods. The patients underwent staging‐laparoscopy by a fellow in gynecologic oncology and a senior surgeon, sequentially and blindly. Single laparoscopic parameters (omental cake, peritoneal and diaphragmatic carcinosis, mesenteric retraction, bowel stomach infiltration, superficial liver metastasis) and a comprehensive laparoscopic score (PIV) were assessed in each procedure and registered. Main outcome measures. No differences in the score discriminating performance for predicting optimal cytoreduction were observed between fellows’ and seniors’ evaluations. Results. The median number of staging laparoscopies performed by each fellow was 30 (range 28–32). The median score was 6 (0–10) for the fellows and 6 (0–14) for senior surgeons (p=ns). Results were superimposable in 57 of 90 patients (63.3%). Dividing the study period into two blocks, cases 1–45 and cases 46–90, differences were equally distributed over time (16.6 vs. 20%; p=0.9). The area under the curve of the receiver operating characteristic (ROC) curves for the score of fellows and seniors was 0.86 and 0.89, respectively (p=ns). Conclusions. The laparoscopic assessment of peritoneal cancer diffusion according to a laparoscopic score can reliably be carried out by a fellow in gynecologic oncology after 12 months’ experience without significant differences from a senior surgeon's assessment.
A pilot study to evaluate the success and survival rate of titanium–zirconium implants in partially edentulous patients: results after 24 months of follow‐up
Authors: Barter Stephen , Stone Paul , Brägger Urs 
Published: June 2011

AbstractObjectives: Implants made from a new titanium–zirconium (TiZr) alloy (Roxolid) have shown good osseointegration with no adverse effects in animal studies. This single‐cohort pilot study was performed to evaluate the performance and safe use of reduced‐diameter implants made from this new TiZr alloy for the first time in human subjects, in a prospective case‐controlled series.Methods: In two private specialist clinics, each of 22 patients received one 3.3 mm TiZr test implant with a Regular Neck Standard Plus design. The use of the new implant was restricted to the indications and protocol for the use of the existing 3.3 mm diameter regular‐neck implant made from Grade IV titanium. The test implants were splinted to a standard Grade IV titanium Regular Neck implant with a fixed dental prosthesis.Results: Twenty of 22 patients had a successful and surviving implant at the 2‐year follow‐up; one study implant was lost 80 days after placement due to infection spreading from an adjacent tooth and one patient did not complete the 2‐year assessment. The mean change in the functional bone level 2 years after loading was −0.33±0.54 mm (−0.32±0.61 mm and −0.34±0.63 mm mesial and distal, respectively). Patients had healthy peri‐implant soft tissues, as indicated by mean probing pocket depths ranging from 2.21 to 2.89 mm after 2 years.Conclusions: Within the limits of this pilot study, the performance of the new implant material was safe and reliable. The new implants meet established success and survival criteria after 2 years.To cite this article: Barter S, Stone P, Brägger U. A pilot study to evaluate the success and survival rate of titanium–zirconium implants in partially edentulous patients: results after 24 months of follow‐up. Clin. Oral Impl. Res. 23, 2012; 873–881. doi: 10.1111/j.1600‐0501.2011.02231.x
The c.273+11dup genetic change in the WAS gene is a functionally neutral polymorphism
Authors: Jeanson‐Leh Laurence , Charrier Sabine , Proust Alexis , Bilhou‐Nabéra Chrystele , Favier Rémi , Deswarte Caroline , Bordigoni Pierre , Galy Anne , Delaunay Jean 
Published: June 2011

AbstractSeveral pediatric patients showing symptoms consistent with the Wiskott–Aldrich syndrome (WAS) were referred to us and turned out to display the c.273+11dup change in the WAS gene. It consisted of the insertion of one C in an unusual tract of 7C near the intron 2 donor splicing site of the WAS gene. In the patients, non‐synonymous WAS mutations were found twice only and one mutation was elucidated in RUNX1. In the absence of a non‐synonymous mutation in the WAS gene, the c.273+11dup change affected neither the levels nor the sequence of WAS mRNA. In the presence of a non‐synonymous WAS mutation, the c.273+11dup alteration failed to worsen the expected phenotype. Minor splicing abnormalities concerning exon 10 were observed both in WAS patients, and in healthy individuals carrying or not carrying the c.273+11dup. The c.273+11dup change was encountered four times in 107 normal male and female controls (172 alleles tested: 2.3%), and eight times in a series of 248 male patients (248 alleles tested: 3.2%). We conclude that the presence of the additional C in the WAS gene is a functionally neutral polymorphism.
Exploring the role of a nonablative laser (1320 nm cooltouch laser) in skin photorejuvenation
Authors: Zhenxiao Zheng , Aie Xu , Yuzhi Jiang , Xiaodong Wei , Xianqiang Jin , Jing Shen , Han Zheng , Junhui Zheng , Xiaojun Zhang , Yanjun Zeng 
Published: June 2011

Background: Nonablative laser has been used for the treatment and prevention of skin aging for many years. Although the mechanism has not been elucidated, histological evaluation showed that the dermal fibroblasts and its collagen production should be the main target for this rejuvenation. In order to determine the effects of a 1320 nm nonablative laser on the human dermal fibroblasts, the two main secretion factors, basic fibroblast growth factor (bFGF) and transforming growth factor β1(TGF‐β1), in vitro were detected.Methods: The human dermal fibroblasts were cultured and irradiated with a 1320 nm laser at the dose of 15, 20, and 24 J/cm2 respectively. The number of fibroblasts was counted and the levels of bFGF and TGF‐β1 were detected by enzyme‐linked immunosorbent assay at the time points of 0, 24, 48, and 72 h after irradiation.Results: The results showed that both the number of fibroblasts and the secretion of bFGF increased after the irradiation at the dose of 20 and 24 J/cm2 (P<0.05) compared with that of the control cells. The bFGF secretion in the group exposed to 20 J/cm2 was more significant than that of 24 J/cm2, and the peak level was 24 h after irradiation. The level of TGF‐β1 secretion decreased after irradiation in a dose‐dependent manner (15 and 20 J/cm2, both P<0.05; 24 J/cm2, P<0.01), and reached a nadir at 24 h.Conclusion: Our results suggested that the 1320 nm nonablative laser accelerates the vitality of fibroblasts, promotes the secretion of bFGF, and inhibits TGF‐β1 secretion by fibroblasts.
DNA methylation in oral squamous cell carcinoma: molecular mechanisms and clinical implications
Authors: González‐Ramírez I , García‐Cuellar C , Sánchez‐Pérez Y , Granados‐García M 
Published: June 2011

Oral Diseases (2011) 17, 771–778DNA methylation is an important regulator of gene transcription, and its role in carcinogenesis has been a topic of considerable interest in the last few years. Of the all epigenetic modifications, methylation, which represses transcription of the promoter region of tumor suppressor genes leading to gene silencing, has been most extensively studied. Oral squamous cell carcinoma (OSCC) has long been known to be the endpoint of many genetic changes, not only genomic mutations but also abnormal epigenetic modifications, as such, promoter methylation, contribute to development of this tumors. Recent studies have shown that promoter methylation of tumor suppressor genes is an important factor in carcinogenesis of OSCC. Some of the main genes that frequently showed promoter methylation in OSCC are those that participate in diverse processes such as regulation of the cell cycle, DNA repair, proliferation, and apoptosis. The aim of this review is to assess the current state of knowledge regarding promoter methylation of diverse genes in OSCC.
Effects of HB‐EGF and epiregulin on wound healing of gingival cells in vitro
Authors: Kim JM , Bak EJ , Chang JY , Kim S‐T , Park W‐S , Yoo Y‐J , Cha J‐H 
Published: June 2011

Oral Diseases (2011) 17, 785–793Objective:  Gingival wound healing is important to periodontal disease and surgery. This in vitro study was conducted to assess the manner in which heparin‐binding epidermal growth factor‐like growth factor (HB‐EGF) and epiregulin cooperatively participate in the wound‐healing process in the gingival epithelial and fibroblast cells of the oral mucosa.Material and Methods:  Gingival epithelium and fibroblast were separated from gingival tissue biopsies and prepared to primary cultures. The changes in the mRNA expression were evaluated via real‐time PCR. The effects on cell proliferation, migration, and repopulation were evaluated in vitro.Results:  The different regulation of expressions of HB‐EGF, epiregulin, and epidermal growth factor receptors was observed over time and with different gingival cell types. HB‐EGF exerted a cell migration‐inducing effect on both epithelial and fibroblast cells, whereas epiregulin did not. Both growth factors functioned as mitogens for epithelial cell proliferation, but not for fibroblast proliferation. HB‐EGF strongly promoted epithelial cell repopulation and mildly promoted fibroblast repopulation, whereas epiregulin promoted only fibroblast repopulation.Conclusion:  These results indicated that both growth factors might function importantly in the wound‐healing process of human gingival tissue via the different regulation of the expression, cell migration, proliferation, and repopulation.
Olanzapine plus fluoxetine treatment alters mitochondrial respiratory chain activity in the rat brain
Authors: Agostinho Fabiano R. , Réus Gislaine Z. , Stringari Roberto B. , Ribeiro Karine F. , Ferreira Gabriela K. , Jeremias Isabela C. , Scaini Giselli , Rezin Gislaine T. , Streck Emílio L. , Quevedo João 
Published: June 2011

Agostinho FR, Réus GZ, Stringari RB, Ribeiro KF, Ferreira GK, Jeremias IC, Scaini G, Rezin GT, Streck EL, Quevedo J. Olanzapine plus fluoxetine treatment alters mitochondrial respiratory chain activity in the rat brain.Background:Evidence is emerging for the role of dysfunctional mitochondria in pathophysiology and treatment of mood disorders. In this study, we evaluated the effects of acute and chronic administration of fluoxetine (FLX), olanzapine (OLZ) and the combination of FLX/OLZ on mitochondrial respiratory chain activity in the rat brain.Methods:For acute treatment, Wistar rats received one single injection of OLZ (3 or 6 mg/kg) and/or FLX (12 or 25 mg/kg) and for chronic treatment, rats received daily injections of OLZ (3 or 6 mg/kg) and/or FLX (12 or 25 mg/kg) for 28 days and we evaluated the activity of mitochondrial respiratory chain complexes I, II, II–III and IV in prefrontal cortex, hippocampus and striatum.Results:Our results showed that both acute and chronic treatments with FLX and OLZ alone or in combination altered respiratory chain complexes activity in the rat brain, but in combination we observed larger alterations.Conclusions:Finally, these findings further support the hypothesis that metabolism energy could be involved in the treatment with antipsychotics and antidepressants in combination to mood disorders.
Depression is associated with lower circulating endothelial progenitor cells and increased inflammatory markers
Authors: Yang Lu , Ruan Lie-Min , Ye Hong-Hua , Cui Han-Bin , Mu Qi-Tian , Lou Yan-Ru , Ji Yun-Xin , Li Wan-Zhen , Sun Ding-He , Chen Xiao-Bei 
Published: June 2011

Yang L, Ruan L-M, Ye H-H, Cui H-B, Mu Q-T, Lou Y-R, Ji Y-X, Li W-Z, Sun D-H, Chen X-B. Depression is associated with lower circulating endothelial progenitor cells and increased inflammatory markers.Objective: To test the hypothesis that depression status in subjects without cardiovascular diseases (CVD) or diabetes is associated with depletion of circulating endothelial progenitor cells (EPCs) and impaired endothelial function.Method: Thirty depressive persons with the first episode of depression (case group) diagnosed according to Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition (DSM-IV) and 30 healthy people (control group) were investigated. The depression status was estimated using Hamilton Rating Scale of Depression from which the criteria of depression are determined to be >21 score. EPCs labeled with CD34-ECD, CD133-phycoerythrin and kinase insert domain receptor (KDR)-fluorescein isothiocyanate antibodies were counted by flow cytometry in the peripheral blood of patients and control subjects. Mononuclear cells that were positive for CD34/KDR, CD133/KDR and CD34/CD133/KDR within the lymphocyte population were characterised as different phenotypes of EPCs.Results: There were no significant differences in baseline clinical characteristics between patients and healthy individuals (all p > 0.05). However, patients with depression had significantly lower levels of circulating CD34+CD133+KDR+ EPCs (132.20 ± 17.27 vs. 225.93 ± 9.88, p = 0.000) and endothelial colony-forming units (26.40 ± 3.79 vs. 36.60 ± 2.88, p = 0.000) than that of healthy subjects. Furthermore, CD34+CD133+KDR+ EPCs had a negative correlation with tumour necrosis factor-α (Spearman's ρ = 0.433, p = 0.000) and interleukin-6 (Spearman's ρ = 0.441, p = 0.032).Conclusion: Our result shows that depression was associated with lower levels of circulating EPCs, which may contribute to the development of endothelial dysfunction and atherosclerosis.
Orthodontic and dentofacial orthopedic management of juvenile idiopathic arthritis: a systematic review of the literature
Authors: von Bremen J , Ruf S 
Published: June 2011

To cite this article:
von Bremen J, Ruf S: Orthodontic and dentofacial orthopedic management of juvenile idiopathic arthritis: a systematic review of the literature Orthod Craniofac Res 2011;14:107–115Structured AbstractAuthors –  von Bremen J, Ruf STo systematically review the literature published on orthodontic treatment principles in patients with juvenile idiopathic arthritis (JIA). Several electronic databases (PubMed, Medpilot, Web of Science, and DIMDI) and orthodontic and rheumatologic literature were systematically searched for studies published until May 2010. The articles were rated by two independent reviewers and included after three selection steps (title–abstract–full text). Articles had to be studies performed on ≥ 5 patients with a disease onset before the age of 16. The selection process resulted in the inclusion of three publications on dentofacial orthopedics and six on combined surgical orthodontic therapy. The three studies on dentofacial orthopedics aimed to improve the mandibular retrusion by means of removable functional appliances (activator). Whereas these orthodontic approaches comprised relatively large and homogeneous patient samples (14, 22, and 72 subjects, aged 6–16), the surgical studies were basically case series with a large age span of the patients (5–12 subjects, aged 10–44). In these surgical treatment approaches, orthodontics was limited to pre‐surgical leveling and post‐surgical finishing, while the skeletal discrepancy was treated surgically by a variety of techniques (costochondral grafts, bilateral sagittal spilt osteotomy, Le Fort I, and genioplasty). The treatment goals of both approaches were improvement of esthetics and function and/or pain reduction, and both approaches showed satisfactory results. Because of the heterogeneity of the subject material and the low level of evidence of the papers, it is difficult to draw any conclusions on the orthodontic/dentofacial orthopedic management of JIA. It appears as if removable functional appliances may be beneficial in adolescent patients with JIA.
Development of a condition‐specific measure to assess quality of life in patients with hypodontia
Authors: Akram AJ , Jerreat AS , Woodford J , Sandy JR , Ireland AJ 
Published: June 2011

To cite this article:
Akram AJ, Jerreat AS, Woodford J, Sandy JR, Ireland AJ: Development of a condition‐specific measure to assess quality of life in patients with hypodontia Orthod Craniofac Res 2011;14:160–167Structured AbstractAuthors –  Akram AJ, Jerreat AS, Woodford J, Sandy JR, Ireland AJObjectives –  To develop a measure to assess quality of life in patients with hypodontia.Setting and Sample Population –  Royal Devon and Exeter NHS Foundation Trust.Materials and Methods –  The study was divided into two parts. The first phase involved developing a patient‐specific questionnaire using 22 patients with hypodontia in five focus groups. The transcripts from these groups were analysed thematically, and emerging themes used to direct the remaining focus groups. When no new themes were identified, it was assumed that data saturation was reached and no further focus groups were organised. In the second phase of the study, a health‐related quality of life questionnaire was developed.Results –  Analysis of transcripts revealed four themes: treatment, effect on daily activities, appearance and other peoples’ reactions. These themes were incorporated into a questionnaire. The readability, ease of administration, face and content validity of the questionnaire were tested.Conclusions –  A new measure for assessment of quality of life in patients with hypodontia is presented.
Improved growth of preterm infants receiving mother’s own raw milk compared with pasteurized donor milk
Authors: Montjaux‐Régis N , Cristini C , Arnaud C , Glorieux I , Vanpee M , Casper C 
Published: June 2011

AbstractAim:  To determine whether growth, feeding tolerance and infectious events of preterm infants is related to the proportion of intake of mother’s own raw milk (maternal milk) versus pooled pasteurized banked breast milk (donor milk).Methods:  This is a prospective observational study of 55 premature infants born less than 32 weeks of gestational age admitted to the neonatal intensive care unit at the Children’s Hospital of Toulouse during two 6‐month periods from 2003 to 2005. Enrolled infants were exclusively on enteral feeds with maternal milk ± donor milk.Results:  Mean gestational age was 28.6 weeks (SD 1.5) and mean birth weight 1105 grams (SD 282). During the time of exclusively breast milk feeds, weight gain (g/kg/day) was correlated to the proportion of maternal milk consumed (p = 0.0048, r = 0.4). Necrotizing enterocolitis was inversely correlated to the amount of maternal milk. The amount of maternal milk did not impact on infectious events.Conclusion:  Mother’s own raw milk improves weight gain compared with donor milk in preterm infants. Lactation strategies should be sought that helps mothers to increase their milk production.
Continuous glucose monitoring system during physical exercise in adolescents with type 1 diabetes
Authors: Adolfsson P , Nilsson S , Lindblad B 
Published: June 2011

AbstractAim:  Continuous glucose monitoring system (CGMS) provides detailed information on glucose fluctuations. The aim was to establish whether CGMS could be used during physical exercise and whether it detects more episodes of hypoglycaemia and hyperglycaemia than frequent blood glucose measurements.Methods:  Adolescents with type 1 diabetes (12 girls and 47 boys) participated in three annual sports camps that lasted for 3–4 days and included different types of exercise: soccer, floorball + cross‐country skiing and golf. During the study, blood glucose values, mean 8.7 ± 3.3 per day, were obtained with Hemocue in parallel with the CGMS.Results:  Ninety‐eight per cent of the participants used the sensor at all times during the camps. Eighty‐seven per cent of the sensors gave adequate signals for 24 h and 66% for 48 h. Median durations of hypoglycaemia and hyperglycaemia were 1.7 h per day and 3.8 h per day, respectively. The CGMS identified significantly more episodes of hypoglycaemia (p < 0.005) and hyperglycaemia (p < 0.005) during the day and night than frequent blood glucose tests.Conclusion:  We demonstrate that, even during days that included episodic strenuous physical exercise, CGMS could provide useful information on glucose fluctuations during day and night, albeit with significant failure rates.
Decadal‐scale aspen changes: evidence in remote sensing and tree ring data
Authors: Sankey Temuulen Tsagaan 
Published: June 2011

Abstract
Question: (1) Which remote sensing classification most successfully identify aspen using multitemporal Landsat 5 TM images and airborne lidar data? (2) How has aspen distribution changed in southwestern Idaho? (3) Are topographic variables and conifer encroachment correlated with aspen changes?
Location: Reynolds Creek Experimental Watershed in southwestern Idaho, USA.
Methods: Multi‐temporal Landsat 5 TM and lidar data were used individually and fused together. The best classification model was compared with a 1965 aspen map and tree ring data. Conifer encroachment was examined via image‐based change detection and field mapping. Lidar‐derived topographic variables were correlated with aspen change patterns using quantile regression models.
Results: The best Landsat 5 TM classification was a normalized difference vegetation index (NDVI)‐based approach with 92% overall accuracy. The lidar classification of tree presence/absence performed with 100% overall accuracy. Fusing the lidar classification with various Landsat 5 TM classifications improved overall accuracies 3 to 6%. Among the fusion models, the NDVI‐lidar fusion performed best with 96% overall accuracy. Change detection indicated 69% decline in aspen cover, but 179% increase in aspen cover in other areas of the watershed. Conifers have completely replaced 17% of the aspen, while 93% of the remaining aspen stands have young Douglas‐fir and western juniper trees underneath the aspen canopy. Aspen significantly decreased (P‐values <0.05) with increasing elevation (up to 2150 m) and decreasing slope.
Conclusions: Landsat 5 TM data used with a NDVI‐based approach provide an accurate method to classify aspen distribution. Landsat 5 TM classifications can be further improved via fusion with lidar data. Aspen change patterns are spatially variable: while aspen is drastically declining in some parts of this watershed, aspen is increasing in other areas.