bluuebunny/crossref_metadata_2025_split huggingface dataset:
SH2‐domain mutations in STAT3 in hyper‐IgE syndrome patients result in impairment of IL‐10 function
Authors: Giacomelli Mauro , Tamassia Nicola , Moratto Daniele , Bertolini Patrizia , Ricci Giampaolo , Bertulli Cristina , Plebani Alessandro , Cassatella Marco , Bazzoni Flavia , Badolato Raffaele 
Published: July 2011

AbstractAutosomal‐dominant hyper‐IgE syndrome (AD‐HIES) is a primary immunodeficiency caused by STAT3 mutations. This inherited condition is characterized by eczema, staphylococcal cold abscesses and recurrent pulmonary infections. Given that STAT3 is involved in IL‐10 signaling, we examined the immunoregulatory role of IL‐10 in inflammation by studying the effects of IL‐10 on monocytes, neutrophils and monocyte‐derived DCs from HIES subjects. Analysis of gene expression in PBMCs and neutrophils isolated from HIES patients and stimulated with LPS in the presence of IL‐10 showed reduced expression of IL1RN, which encodes IL‐1 receptor antagonist (IL‐1ra), and SOCS3 mRNA but increased CXCL8 mRNA expression. Moreover, secretion of the anti‐inflammatory protein IL‐1ra was reduced in AD‐HIES patients. DCs from HIES patients secreted higher levels of TNF‐α, IL‐6 and, to a lesser extent, IL‐12 when these cells were cultured in the presence of IL‐10. These results suggest that IL‐10 activity is affected in myeloid cells (e.g. monocytes, DCs) of HIES patients. Impairment of IL‐10 signaling in patients with AD‐HIES might result in an altered balance between pro‐inflammatory and anti‐inflammatory signals and might lead to persistent inflammation and delayed healing after infections.
Copper‐Catalyzed Domino One‐Pot Synthesis of 2‐(Arylselanyl)arylcyanamides
Authors: Ramana Tamminana , Punniyamurthy Tharmalingam 
Published: July 2011

AbstractDomino C–Se cross‐coupling of 2‐(iodoaryl)selenoureas with aryl iodides has been accomplished in the presence of a copper(I)–1,1‐phenanthroline complex at moderate temperature. The reactions involve intra‐ and intermolecular C–Se cross‐coupling to give the substituted 2‐(arylselanyl)arylcyanamides. Aryl iodides having electron‐donating and ‐withdrawing substituents are compatible, affording the targetselanyl ethers in high yield.
Controls of microalgal biomass and lipid production in municipal wastewater‐fed bioreactors
Authors: Sturm Belinda S.M. , Peltier Edward , Smith Val , deNoyelles Frank 
Published: July 2011

AbstractThe use of wastewater as a nutrient source for algal growth as a biodiesel feedstock could increase the viability of this process and reduce wastewater discharge of nitrogen‐ and phosphorus‐bearing compounds. In this study, four algal bioreactors were fed continuously with municipal wastewater effluent over a 6‐month period; these were monitored weekly to determine algal biomass and lipid production, nutrient removal, and carbon and nutrient availability. Algal biomass production ranged from 0.78 to 15.9 g dry weight m−2 d−1 over the experimental period. Algal reactors removed 19% of dissolved nitrogen and 43% of dissolved phosphorus from wastewater effluent. A stoichiometric analysis of particulates revealed that algal biomass had far lower C:P ratios (67:1) and N:P ratios (6:1), but higher C:N ratios (17:1), than Redfield values. These data suggest that the algae produced were rich in phosphorus, and that nitrogen was likely to be growth‐limiting in these systems. Nonetheless, numerous studies suggest that algal biomass can be enhanced by providing CO2 in excess of normal atmospheric concentrations, and that algal biofuel production can contribute to CO2 sequestration efforts. To optimize nutrient removal in wastewater‐fed algal reactors, and to determine the potential for CO2 sequestration, future reactor engineering research should continue to incorporate the expertise of ecological stoichiometry. © 2011 American Institute of Chemical Engineers Environ Prog, 2012
Motivation‐focused Treatment for Eating Disorders: A Sequential Trial of Enhanced Cognitive Behaviour Therapy with and without Preceding Motivation‐Focused Therapy
Authors: Allen Karina L. , Fursland Anthea , Raykos Bronwyn , Steele Anna , Watson Hunna , Byrne Susan M. 
Published: July 2011

AbstractObjectiveTo evaluate the effectiveness of a motivation‐focused intervention prior to individual cognitive behavioural eating disorder treatment.MethodEnhanced cognitive–behavioural therapy (CBT‐E) in its usual form was compared with CBT‐E preceded by four sessions of motivation‐focused therapy (MFT) (MFT + CBT‐E). Participants were adult outpatients seen at a specialist eating disorder clinic in Western Australia, who met criteria for a Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition eating disorder. A sequential trial of CBT‐E as usual (n = 43) and MFT + CBT‐E (n = 52) was conducted over a 40‐month period.ResultsThe MFT phase was associated with significant increases in readiness to change. There were no significant between‐group differences in treatment completion rates, and treatment completers in both conditions reported comparable reductions in eating disorder symptoms over time.ConclusionIn this sample, MFT + CBT‐E was not associated with superior treatment outcome when compared with CBT‐E as usual. Copyright © 2011 John Wiley & Sons, Ltd and Eating Disorders Association.
The Meaning of Food (MOF): The Development of a New Measurement Tool
Authors: Ogden Jane , Liakopoulou Efi , Antilliou George , Gough Gary 
Published: July 2011

AbstractThis paper aimed to develop a reliable measurement tool to evaluate the meanings of food that could be used in both practice and research and to examine possible gender differences. A new meaning of food questionnaire (MOF) was refined across two studies (study 1, n = 451 and study 2, n = 170). The final questionnaire consisted of 25 items and 8 reliable subscales: food and sex, emotional regulation, treat, guilt, social interaction, control over life, control over food, family. The new Meaning of Food (MOF) questionnaire could be used in both research and clinical practice to profile patients and explore predictors of eating behaviour. Copyright © 2011 John Wiley & Sons, Ltd and Eating Disorders Association.
Effect of dynamic load on heat transfer characteristic of a two‐phase pipe boiling flow
Authors: Yao Qiuping , Song Baoyin , Zhao Mei 
Published: July 2011

AbstractAn experimental investigation was performed to obtain the flow and heat transfer characteristics of a single‐phase water flow and a two‐phase pipe boiling water flow under dynamic load in the present work. By analyzing the fluid resistance, effective heat, flow pattern, and heat transfer coefficient of the experimental data, the effects of dynamic load on the flow and heat transfer characteristics of single‐phase water and two‐phase boiling water flow were investigated. The results show that the dynamic load significantly influences the flow characteristic and boiling heat transfer of the two‐phase pipe flow. It will enhance the fluid resistance and heat dissipation toward the ambient environment, and reduce the heat transferred to the two‐phase fluid. The impact mixing flow caused by the dynamic load breaks the uniform and varying principle of the wall temperatures. As a result of that, the greater the dynamic load, the lower the wall inner bottom temperature and the higher the wall inner top temperature in a certain extent. © 2011 Wiley Periodicals, Inc. Heat Trans Asian Res; Published online in Wiley Online Library (wileyonlinelibrary.com/journal/htj). DOI 10.1002/htj.20378
Mechanisms of FGFR3 actions in endocrine resistant breast cancer
Authors: Tomlinson D.C. , Knowles M.A. , Speirs V. 
Published: July 2011

AbstractAlthough endocrine therapy has dramatically improved the treatment of breast cancer therapeutic resistance and tumour recurrence occurs, even in estrogen receptor (ER) positive cases. Identifying and understanding the molecular mechanisms which underpin endocrine resistance is therefore important if future therapeutic strategies are to be developed. Members of the fibroblast growth factor (FGF) and fibroblast growth factor receptor (FGFR) families have been implicated in breast cancer development and progression. Our results demonstrate that culture of michigan cancer foundation ‐ 1 (MCF)7 cells with FGF1 results in reduced sensitivity to tamoxifen in vitro. Furthermore, our tissue microarray expression data demonstrates that FGFR3 expression is increased in tamoxifen resistant breast tumours. To confirm that activation of FGFR3 reduced sensitivity to tamoxifen we used an inducible activation system and a constitutively active mutant of FGFR3 expressed in MCF7 cells. Activation of FGFR3 reduced sensitivity to tamoxifen and Fulvestrant but did not lead to phosphorylation of ER demonstrating that FGFR3 does not feedback to modulate ER activity. FGFR3 activation in MCF7 cells stimulated activation of the mitogen‐activated protein kinase (MAPK) and phosphoinositide 3‐kinase (PI3K) signalling pathways, both of which have been implicated in tamoxifen resistance in breast cancer. Furthermore, our data indicates that activation of phospholipase C gamma is a key‐signalling event regulating MAPK and PI3K activation and that its activation reduces sensitivity to tamoxifen. Therefore, we hypothesise that FGFRs could play an integral part, not only in breast cancer development but also in resistance to endocrine‐therapy.
Luteolin induces apoptosis in multidrug resistant cancer cells without affecting the drug transporter function: Involvement of cell line‐specific apoptotic mechanisms
Authors: Rao Prema S. , Satelli Arun , Moridani Majid , Jenkins Marjorie , Rao U. Subrahmanyeswara 
Published: July 2011

AbstractBioflavonoids are of considerable interest to human health as these serve as antioxidant and anticancer agents. Although epidemiological and experimental studies suggest that luteolin, a natural bioflavonoid, exhibits chemopreventive properties, its effectiveness as an antiproliferative agent against multidrug resistant (MDR) cancers is unclear. Thus, we assessed the antiproliferative effects of luteolin and associated molecular mechanisms using two MDR cancer cell lines that express high levels of P‐glycoprotein and ABCG2. In this article, we demonstrate that luteolin induces apoptosis in P‐glycoprotein‐ and ABCG2‐expressing MDR cancer cells without affecting the transport functions of these drug transporters. Analysis of various proliferative signaling pathways indicated that luteolin‐induced apoptosis involves reactive oxygen species generation, DNA damage, activation of ATR → Chk2 → p53 signaling pathway, inhibition of NF‐kB signaling pathway, activation of p38 pathway and depletion of antiapoptotic proteins. Importantly, use of luteolin in these analyses also identified specific molecular characteristics of NCI‐ADR/RES and MCF‐7/MitoR cells that highlight their different tissue origins. These results suggest that luteolin possesses therapeutic potential to control the proliferation of MDR cancers without affecting the physiological function of drug transporters in the body tissues.
Toward standardizing and reporting colorectal cancer screening indicators on an international level: The international colorectal cancer screening network
Authors: Benson Victoria S. , Atkin Wendy S. , Green Jane , Nadel Marion R. , Patnick Julietta , Smith Robert A. , Villain Patricia ,   on behalf of the International Colorectal Cancer Screening Network
Published: July 2011

AbstractThe International Colorectal Cancer Screening Network was established in 2003 to promote best practice in the delivery of organized colorectal cancer screening programs. To facilitate evaluation of such programs, we defined a set of universally applicable colorectal cancer screening measures and indicators. To test the feasibility of data collection, we requested data on these variables and basic program characteristics from 26 organized full programs and 9 pilot programs in 24 countries. The size of the target population for each program varied considerably from a few thousand to 36 million. The majority of programs used fecal occult blood tests for primary screening, with more using guaiac than immunochemical tests. There was wide variation in the ability of screening programs to report the requested measures and in the values reported. In general, pilot programs were more likely to provide screening measure values than were full programs. As expected, detection rates for polyps and neoplasia were substantially higher in programs screening with endoscopy than in those using fecal occult blood tests. It is hoped that the screening measures and indicators, once revised in the light of this survey, will be adopted and used by existing programs and those in the early planning stages, allowing international comparison with the goal of improved colorectal cancer screening quality.
Progress against cancer in the Netherlands since the late 1980s: An epidemiological evaluation
Authors: Karim‐Kos Henrike E. , Kiemeney Lambertus A.L.M. , Louwman Marieke W.J. , Coebergh Jan Willem W. , de Vries Esther 
Published: July 2011

AbstractProgress against cancer through prevention and treatment is often measured by survival statistics only instead of analyzing trends in incidence, survival and mortality simultaneously because of interactive influences. This study combines these parameters of major cancers to provide an overview of the progress achieved in the Netherlands since 1989 and to establish in which areas action is needed. The population‐based Netherlands Cancer Registry and Statistics Netherlands provided incidence, 5‐year relative survival and mortality of 23 major cancer types. Incidence, survival and mortality changes were calculated as the estimated annual percentage change. Optimal progress was defined as decreasing incidence and/or improving survival accompanied by declining mortality, and deterioration as increasing incidence and/or deteriorating survival accompanied by increasing mortality rates. Optimal progress was observed in 12 of 19 cancer types among males: laryngeal, lung, stomach, gallbladder, colon, rectal, bladder, prostate and thyroid cancer, leukemia, Hodgkin and non‐Hodgkin lymphoma. Among females, optimal progress was observed in 12 of 21 cancers: stomach, gallbladder, colon, rectal, breast, cervical, uterus, ovarian and thyroid cancer, leukemia, Hodgkin and non‐Hodgkin lymphoma. Deterioration occurred in three cancer types among males: skin melanoma, esophageal and kidney cancer, and among females six cancer types: skin melanoma, oral cavity, pharyngeal, esophageal, pancreatic and lung cancer. Our conceptual framework limits misinterpretations from separate trends and generates a more balanced discussion on progress.
Polymerizable superoxide dismutase mimetic protects cells encapsulated in poly(ethylene glycol) hydrogels from reactive oxygen species‐mediated damage
Authors: Hume Patrick S. , Anseth Kristi S. 
Published: July 2011

AbstractA polymerizable superoxide dismutase mimetic (SODm) was incorporated into poly(ethylene glycol) (PEG) hydrogels to protect encapsulated cells from superoxide‐mediated damage. Superoxide and other small reactive oxygen species (ROS) can cause oxidative damage to donor tissue encapsulated within size exclusion barrier materials. To enzymatically breakdown ROS within biomaterial cell encapsulation systems, Mn(III) Tetrakis[1‐(3‐acryloxy‐propyl)‐4‐pyridyl] porphyrin (MnTTPyP‐acryl), a polymerizable manganese metalloporphyrin SOD mimetic, was photopolymerized with PEG diacrylate (PEGDA) to create functional gels. In unmodified PEG hydrogels, a significant reduction in metabolic activity was observed when encapsulated Min6 β‐cells were challenged with chemically generated superoxide. Cells encapsulated within MnTPPyP‐co‐PEG hydrogels, however, demonstrated greatly improved metabolic activity following various superoxide challenges. Further, cells were encapsulated and cultured for 10 days within MnTPPyP‐co‐PEG hydrogels and challenged with superoxide on days 4, 6, and 8. At the conclusion of this study, cells in blank PEG hydrogels had no observable metabolic activity but when encapsulated in MnTPPyP‐functionalized hydrogels, cells retained 60 ± 5% of the metabolic activity compared to untreated controls. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 99A: 29–37, 2011.
Covalent stabilization of alginate hydrogel beads via Staudinger ligation: Assessment of poly(ethylene glycol) and alginate cross‐linkers
Authors: Gattás‐Asfura Kerim M. , Fraker Christopher A. , Stabler Cherie L. 
Published: July 2011

AbstractCellular encapsulation within alginate hydrogel capsules has broad applications in tissue engineering. In seeking to improve the inherent instability of ionically cross‐linked alginate hydrogels, we previously demonstrated the covalent stabilization of Ba2+ cross‐linked alginate‐azide beads via chemoselective Staudinger ligation using a 1‐methyl‐2‐diphenylphosphino‐terephthalate (MDT) terminated poly(ethylene glycol) (PEG) linker. In this study, we functionalized variant PEG, linear and branched, and alginate polymers with MDT groups to evaluate the effect of size, structural design, number of functional groups, and charge on the resulting hydrogel bead. All cross‐linkers resulted in enhanced covalent stabilization of alginate beads, with significant decreases in swelling and resistance to dissolution via Ba2+ chelation. The MDT‐functionalized alginate resulted in the most stable and homogeneous bead, with the most restrictive permeability even after EDTA exposure. Co‐encapsulation of MIN6 cells within the cross‐linked alginate hydrogel beads resulted in minimal effects on viability, whereas the degree of proliferation following culture varied with cross‐linker type. Altogether, the results illustrate that manipulating the cross‐linker structural design permits flexibility in resulting alginate beads characteristics. Covalent stabilization of alginate hydrogel beads with these chemoselective alginate and PEG‐based cross‐linkers provides a unique platform for cellular encapsulation. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 99A: 47–57, 2011.
Reconsideration on hydrogen bond strengthening or cleavage of photoexcited coumarin 102 in aqueous solvent: A DFT/TDDFT study
Authors: Miao Chuang , Shi Ying 
Published: July 2011

AbstractIn this work, the excited‐state hydrogen bonding dynamics of photoexcited coumarin 102 in aqueous solvent is reconsidered. The electronically excited states of the hydrogen bonded complexes formed by coumarin 102 (C102) chromophore and the hydrogen donating water solvent have been investigated using the time‐dependent density functional theory method. Two intermolecular hydrogen bonds between C102 and water molecules are considered. The previous works (Wells et al., J Phys Chem A 2008, 112, 2511) have proposed that one intermolecular hydrogen bond would be strengthened and the other one would be cleaved upon photoexcitation to the electronically excited states. However, our theoretical calculations have demonstrated that both the two intermolecular hydrogen bonds between C102 solute and H2O solvent molecules are significantly strengthened in electronically excited states by comparison with those in ground state. Hence, we have confirmed again that intermolecular hydrogen bonds between C102 chromophore and aqueous solvents are strengthened not cleaved upon electronic excitation, which is in accordance with Zhao's works. © 2011 Wiley Periodicals, Inc. J Comput Chem, 2011
The Use and Efficacy of Empathy in Police Interviews with Suspects of Sexual Offences
Authors: Oxburgh Gavin , Ost James 
Published: July 2011

AbstractMany researchers have argued that the use of empathy in police interviews is beneficial to the rapport building process, with some arguing that its use may actually increase the number of admissions from specific cohorts of suspected offenders. Many police training protocols and guidelines also suggest that officers should use empathy during investigative interviews, yet no distinct definition is provided. This paper will provide a review of the current literature in the area and will discuss the meaning of empathy and its effectiveness during police interviews with those suspected of committing sexual offences. Copyright © 2011 John Wiley & Sons, Ltd.
Interviewing Suspects of Crime: The Impact of PEACE Training, Supervision and the Presence of a Legal Advisor
Authors: Clarke Colin , Milne Rebecca , Bull Ray 
Published: July 2011

AbstractIn 1992, the police service of England and Wales adopted PEACE as a framework for interviewing suspects. This study was to evaluate the impact of PEACE interview training, workplace supervision and the presence of a legal advisor on the performance of police officers' interviewing suspects. One hundred seventy‐four real‐life interviews with suspects were obtained from six police forces in England and Wales. Officers trained and untrained in PEACE, and from police forces that did or did not have an interview supervision policy conducted the interviews. Interviews in this sample were generally of average standard. Whilst PEACE‐trained officers conducted longer interviews, there were no other statistical differences dependent on training. Nor were there any statistical differences dependent on the presence of a legal advisor. A workplace supervision policy was found to be related to performance, particularly during the engage and explain phases of the interview. Supervision also appeared to provide some safeguards for suspects who did not have a legal advisor. Because the performance of PEACE‐trained officers did not differ from those who had not received this training, further improvement in training is needed, especially regarding a number of communication skills. It is argued that such improvement would be facilitated by a more consistent approach to interviewer training and to the effective supervision of interviews. Copyright © 2011 John Wiley & Sons, Ltd.
Changes and function of circulating endothelial progenitor cells in patients with cerebral aneurysm
Authors: Wei Huijie , Mao Qun , Liu Li , Xu Yong , Chen Jieli , Jiang Rongcai , Yin Long , Fan Yimu , Chopp Michael , Dong Jingfei , Zhang Jianning 
Published: July 2011

AbstractEndothelial dysfunction is a trigger for the formation of cerebral aneurysm (CA). The circulating endothelial progenitor cell (EPC) plays an important role in postnatal vasculogenesis and reduction of endothelial injury. In this study, we tested the hypothesis that decreased number and impaired function of circulating EPCs correlate with CA formation in patients. Blood circulating EPCs were identified by flow cytometry. The level of plasma vascular endothelial growth factor (VEGF) was measured by ELISA. Circulating EPCs from patients (n = 27) were cultured in vitro, and the function of EPCs was evaluated by cell migration and senescence‐associated β‐galactosidase activity. The number of circulating EPCs was significantly decreased in both unruptured and ruptured CA patients compared with healthy control subjects. Impaired migratory capacity and elevated cellular senescence of cultured EPCs were observed in patients with CA (ruptured and unruptured). The percentages of EPC senescence in patients with CAs were significantly and negatively correlated with the number of circulating EPCs. In addition, there were higher levels of plasma VEGF in CA patients compared with healthy control subjects. Our results show that the numbers and functions of circulating EPCs are reduced in patients with CAs. These findings suggest that the decreased number and impaired function of circulating EPCs in CA patients may contribute to the pathophysiological process of aneurysm formation. © 2011 Wiley‐Liss, Inc.