bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stem cell research in Asia: A critical view
Authors: Sipp Douglas 
Published: April 2009

AbstractStem cell research stands as a high‐priority field in many countries across the Asia‐Pacific region, and the past decade has seen remarkable investment into facilities and programs intended to increase competitiveness in the drive to find clinical applications. In the years roughly framed by Korean cloner Woo‐Suk Hwang's meteoric ascent and fall, speculation was rampant that Asia was poised to overtake the West in this field of science. But that potential remains unfulfilled. In this article, I will look at some of the deficits in infrastructure and governance that underlie the East–West stem cell gap, and suggest a number of measures that might be taken to remedy them. J. Cell. Biochem. 107: 853–856, 2009. © 2009 Wiley‐Liss, Inc.
Evidence for direct effects of prolactin on human osteoblasts: Inhibition of cell growth and mineralization
Authors: Seriwatanachai Dutmanee , Krishnamra Nateetip , van Leeuwen J.P.T.M. 
Published: April 2009

AbstractHyperprolactinemia is one of the risk factor of decrease in bone mass which has been believed to be mediated by hypogonadism. However, the presence of prolactin receptor in human osteosarcoma cell line and primary bone cell culture from mouse calvariae supported the hypothesis of a direct prolactin (PRL) action on bone cells. Therefore, the aim of this study was to investigate the role of PRL and its signal transduction pathway in the regulation of bone metabolism via osteoblast differentiation. Human pre‐osteoblasts (SV‐HFO) that differentiate in a 3‐week period from proliferating pre‐osteoblasts (days 2–7) to extracellular matrix producing cells (days 7–14) which is eventually mineralized (days 14–21) were used. Concentration of PRL mimicked a lactating period (100 ng/ml) was used to incubate SV‐HFO for 21 days in osteogenic medium. Human prolactin receptor mRNA and protein are expressed in SV‐HFO. PRL significantly decreased osteoblast number (DNA content) which was due to a decrease in proliferation. PRL increased osteogenic markers, RUNX2 and ALP in early stage of osteoblast differentiation while decreasing it later suggesting a bi‐directional effect. Calcium measurement and Alizarin red staining showed a reduction of mineralization by PRL while having neither an effect on osteoblast activity nor RANKL/OPG mRNA ratio. We also demonstrated that PRL action on mineralization was not via PI‐3 kinase pathway. The present study provides evidence of a direct effect of prolactin on osteoblast differentiation and in vitro mineralization. J. Cell. Biochem. 107: 677–685, 2009. © 2009 Wiley‐Liss, Inc.
Contact inhibition revisited
Authors: Heckman Carol A. 
Published: April 2009

AbstractContact inhibition of cell movement was originally defined in the 1950s as a way of interpreting studies that were ethological and statistical in nature. Research done in succeeding decades provided a more detailed study of the initial contact and its consequences for the cell. The behavior called contact inhibition is characterized by the cessation of ruffling and forward movement in the lamellipodium of the cell making the contact. A new ruffling membrane then arises elsewhere on the cell perimeter. A comparison between the contact behavior described in the early literature and that of the nerve growth cone, described recently by Steketee and Tosney, suggests that filopodia mediate the sensing function in both cases. Since transformed cells have fewer filopodia than normal cells, the contact behavior may decline in direct response to the degraded function of filopodia. This new “filopodia focal signal transduction” hypothesis of contact inhibition elevates the filopodia sensing function and the cessation of lamellipodial advance to the highest importance as phenomena underlying the altered behavior of cancer cells. J. Cell. Physiol. 220: 574–575, 2009. © 2009 Wiley‐Liss, Inc.
NESCA: A new NEMO/IKKgamma and TRAF6 interacting protein
Authors: Napolitano Gennaro , Mirra Serena , Monfregola Jlenia , Lavorgna Alfonso , Leonardi Antonio , Ursini Matilde Valeria 
Published: April 2009

AbstractNEMO/IKKγ is the essential regulatory subunit of the IkB Kinase (IKK) complex, required for the activation of Nuclear Factor kB (NF‐kB) in many physiological processes such as inflammation, immunity, apoptosis, or development. NEMO works at a converging point of the NF‐kB pathway as it interacts with upstream signaling molecules to orchestrate its activation. Here we report on the identification of a novel NEMO‐interacting protein, NESCA, an adapter molecule previously shown to be involved in the NGF‐pathway via the TrkA receptor. We demonstrated that NESCA and NEMO interact by their N‐terminal region. Beside to NEMO, we revealed that NESCA directly associates to the E3 ubiquitin ligase TRAF6, which in turn catalyzes NESCA polyubiquitination. Finally, we demonstrated that NESCA overexpression strongly inhibits TRAF6‐mediated polyubiquitination of NEMO. In summary, our results highlight that NESCA represents a novel missing link in the NEMO‐mediated NF‐kB activation pathway. J. Cell. Physiol. 220: 410–417, 2009. © 2009 Wiley‐Liss, Inc.
Proteasome inhibitors impair RANKL‐induced NF‐κB activity in osteoclast‐like cells via disruption of p62, TRAF6, CYLD, and IκBα signaling cascades
Authors: Ang Estabelle , Pavlos Nathan J. , Rea Sarah L. , Qi Ming , Chai Tracy , Walsh John P. , Ratajczak Thomas , Zheng Ming H. , Xu Jiake 
Published: April 2009

AbstractProteasome inhibitors represent a promising therapy for the treatment of relapsed and/or refractory multiple myeloma, a disease that is concomitant with osteolysis and enhanced osteoclast formation. While blockade of the proteosome pathway has been recently shown to influence osteoclast formation and function, the precise molecular cascade underlying these effects is presently unclear. Here, we provide evidence that proteasome inhibitors directly impair osteoclast formation and function via the disruption of key RANK‐mediated signaling cascades. Disruption of the proteosome pathway using selective inhibitors (MG‐132, MG‐115, and epoxomicin) resulted in the accumulation of p62 and CYLD, and altered the subcellular targeting and distribution of p62 and TRAF6 in osteoclast‐like cells. Proteosome inhibition also blocked RANKL‐induced NF‐κB activation, IκBα degradation and nuclear translocation of p65. The disruption in RANK‐signaling correlated dose‐dependently with an impairment in osteoclastogenesis, with relative potency epoxomicin > MG‐132 > MG‐115 based on equimolar concentrations. In addition, these inhibitors were found to impact osteoclastic microtubule organization and attenuate bone resorption. Based on these data we propose that deregulation of key RANK‐mediated signaling cascades (p62, TRAF6, CYLD, and IκBα) underscores proteasome‐mediated inhibition of osteolytic bone conditions. J. Cell. Physiol. 220: 450–459, 2009. © 2009 Wiley‐Liss, Inc.
Rainbow faces in edge‐colored plane graphs
Authors: Jendrol' Stanislav , Miškuf Jozef , Soták Roman , Škrabul'áková Erika 
Published: April 2009

AbstractA face of an edge‐colored plane graph is called rainbow if the number of colors used on its edges is equal to its size. The maximum number of colors used in an edge coloring of a connected plane graph Gwith no rainbow face is called the edge‐rainbowness of G. In this paper we prove that the edge‐rainbowness of Gequals the maximum number of edges of a connected bridge face factor H of G, where a bridge face factor H of a plane graph Gis a spanning subgraph H of Gin which every face is incident with a bridge and the interior of any one face f∈F(G) is a subset of the interior of some face f′∈F(H). We also show upper and lower bounds on the edge‐rainbowness of graphs based on edge connectivity, girth of the dual graphs, and other basic graph invariants. Moreover, we present infinite classes of graphs where these equalities are attained. © 2009 Wiley Periodicals, Inc. J Graph Theory 62: 84–99, 2009
Spanning even subgraphs of 3‐edge‐connected graphs
Authors: Jackson Bill , Yoshimoto Kiyoshi 
Published: April 2009

AbstractBy Petersen's theorem, a bridgeless cubic graph has a 2‐factor. H. Fleischner extended this result to bridgeless graphs of minimum degree at least three by showing that every such graph has a spanning even subgraph. Our main result is that, under the stronger hypothesis of 3‐edge‐connectivity, we can find a spanning even subgraph in which every component has at least five vertices. We show that this is in some sense best possible by constructing an infinite family of 3‐edge‐connected graphs in which every spanning even subgraph has a 5‐cycle as a component. © 2009 Wiley Periodicals, Inc. J Graph Theory 62: 37–47, 2009
The complexity of the matching‐cut problem for planar graphs and other graph classes
Authors: Bonsma Paul 
Published: April 2009

AbstractThe Matching‐Cut problem is the problem to decide whether a graph has an edge cut that is also a matching. Previously this problem was studied under the name of the Decomposable Graph Recognition problem, and proved to be
${\cal{NP}}$‐complete when restricted to graphs with maximum degree four. In this paper it is shown that the problem remains
${\cal{NP}}$‐complete for planar graphs with maximum degree four, answering a question by Patrignani and Pizzonia. It is also shown that the problem is
${\cal{NP}}$‐complete for planar graphs with girth five. The reduction is from planar graph 3‐colorability and differs from earlier reductions. In addition, for certain graph classes polynomial time algorithms to find matching‐cuts are described. These classes include claw‐free graphs, co‐graphs, and graphs with fixed bounded tree‐width or clique‐width. © 2009 Wiley Periodicals, Inc. J Graph Theory 62: 109–126, 2009
On uniqueness of a general factorization of graph properties
Authors: Drgas‐Burchardt Ewa 
Published: April 2009

AbstractA graph property is any class of simple graphs, which is closed under isomorphisms. Let H be a given graph on vertices v1, …, vn. For graph properties
𝒫1, …, 𝒫n, we denote by H[𝒫1, …, 𝒫n] the class of those (𝒫1, …, 𝒫n) ‐partitionable graphs G, with a corresponding vertex partition (V1, …, Vn), for which an edge {xi, xj} with xi∈Vi and xj∈Vj implies the existence of the edge {vi, vj} in the graph H. The problem of the unique description of a graph property 𝒫 in the form H[𝒫1, …, 𝒫n] is investigated for 𝒫, 𝒫1, …, 𝒫n being from the class La of all graph properties closed under taking disjoint unions and subgraphs. The unique factorization theorems obtained in the paper generalize known results of this type bringing together ∘ ‐reducibility over La and ∨ ‐reducibility in the lattice (La, ⊆). There is also offered a new insight into the modular decomposition tree for a graph. © 2009 Wiley Periodicals, Inc. J Graph Theory 62: 48–64, 2009
A local tournament contains a vertex whose out‐arcs are pseudo‐girth‐pancyclic
Authors: Meng Wei , Li Shengjia , Guo Yubao , Xu Gaokui 
Published: April 2009

AbstractAn arc leaving a vertex x in a digraph is called an out‐arc of x. Thomassen (J Combin Theory Ser B 28 (1980), 142–163) proved that every strong tournament contains a vertex whose every out‐arc is contained in a Hamiltonian cycle. In 2000, Yao et al. (Discrete Appl Math 99 (2000), 245–249) improved the result of Thomassen and confirmed that every strong tournament contains a vertex whose out‐arcs are pancyclic. In this article, we extend the result of Yao et al. to local tournaments and obtain a best possible result in some sense. © 2009 Wiley Periodicals, Inc. J Graph Theory 62: 346–361, 2009
Regioselective synthesis and bioactivity of new 5‐amino‐6‐arylamino‐pyrazolo[3,4‐d]‐pyrimidin‐4(5H)‐one derivatives
Authors: Wang Hong‐Qing , Zhou Wei‐Ping , Wang Yu‐Yuan , Lin Can‐Rong , Liu Zhao‐Jie 
Published: April 2009

Abstract
magnified imageA novel approach to regioselective synthesis of new 5‐amino‐6‐arylamino‐1H‐pyrazolo[3,4‐d]pyrimidin‐4(5H)‐one 5 derivatives via a tandem aza‐wittig and annulation reaction of iminophosphorance 2, aromatic isocyanates and hydrazine in 69.6–94.7% isolated yields is reported. The compound 5 reacted with triethyl orthoformate to give compound 6 in good yield (65.8–82.8%). Their structure was clearly confirmed by spectroscopy data (IR, 1H NMR, MS, elemental analysis) and the results of preliminary bioassay indicated that compounds 5 and 6 possess high antifungal activity against Botrytis cinerea Pers and Sclerotinia sclerotiorum, and compound 5h showed 100, 96.4, and 90.2% inhibitory rate to Botrytis cinerea Pers, Pyricularia oryzae, and Sclerotinia sclerotiorum at the concentration of 50 mg/L. The antifungal activities of compound 6 were generally higher than those of compound 5. J. Heterocyclic Chem., (2009).
Bis(enaminones): Key intermediates for novel α,ω‐bis(pyrazolylphenoxy), bis(pyranylphenoxy), and bis(benzo[b]furanylphenoxy) alkanes
Authors: Abbas Ashraf A. 
Published: April 2009

Abstract
magnified imageNew bis(enaminone) derivatives, 5a, 5b and 9a, 9b, were prepared in good yields. Their synthetic utilities as key intermediates for the synthesis of novel bis(pyrazole) 12a ,12b, bis(pyrane) 17a ,17b, and bis(benzo[b]furan) 20a, 20b, 20c, 20d derivatives were also investigated. J. Heterocyclic Chem., 46, 340 (2009).
Synthesis and antimicrobial activity of novel analogs of trifenagrel
Authors: Nagarapu Lingaiah , Aneesa  , Satyender Apuri , Chandana G. , Bantu Rajaskaker 
Published: April 2009

Abstractmagnified imageNovel analogs of trifenagrel were synthesized by using inexpensive and reusable phosphotungstic acid, H3[PW12O40] (3 mol %) catalyst under classical heating. Two of the newly synthesized triaryl imidazoles exhibited moderate antibacterial activity. J. Heterocyclic Chem.,46, 195 (2009).
Preparation of 2H‐spiro[benzo[d]isothiazole‐3,3′‐pyrazole]‐1, 1‐dioxide‐2′(4′H)‐carboxylates from dilithiated C(α),N‐carboalkoxyhydrazones and methyl 2‐(aminosulfonyl)benzoate
Authors: Dawsey Anna C. , Potter Chandra , Knight John D. , Kennedy Zachary C. , Smith Ellyn A. , Acevedo‐Jake Amanda M. , Puciaty Andrew J. , Metz Clyde R. , Beam Charles F. , Pennington William T. , VanDerveer Donald G. 
Published: April 2009

Abstract
magnified imageA variety of substituted spiro(benzoisothiazole‐pyrazoles) have been prepared by the condensation of dilithiated C(α),N‐carboalkoxyhydrazones with lithiated methyl 2‐(aminosulfonyl)benzoate followed by the cyclization of intermediates with acetic anhydride, which also resulted in spiro N‐acetylated products when carbomethoxyhydrazones or carboethoxyhydrazones were used, and spiro NH products when carbo‐tert‐butoxyhydrazones were used. J. Heterocyclic Chem., 46, 231 (2009).
A new synthetic route to 2H‐benzo[g]chromene‐5,10‐diones involving ring closing metathesis
Authors: Song Young Seok , Lee Kee‐Jung 
Published: April 2009

Abstract
magnified imageA simple synthesis of several 2H‐benzo[g]chromene‐5,10‐diones via the ring closing metathesis reaction of prerequisite bisolefins, prepared from the α‐vinylnaphthoquinones, is described. J. Heterocyclic Chem., 46, 207 (2009).
An efficient synthesis of pyrimido[4,5‐b]quinoline derivativesviathree‐component reaction in aqueous media
Authors: Shi Da‐Qing , Niu Li‐Hui , Yao Hao , Jiang Hong 
Published: April 2009

Abstractmagnified imageA series of pyrimido[4,5‐b]quinoline derivatives was synthesized by three‐component reaction of 6‐aminopyrimidine, aromatic aldehydes and 1,3‐cyclohexanedione or 5,5‐dimethyl‐1,3‐cyclohexanedione in aqueous media in the presence of triethylbenzylammonium chloride. This protocol has the advantages of higher yields, lower lost, easy work‐up, and environmentally friendly procedure. J. Heterocyclic Chem.,46, 237 (2009).
Facile, microwave‐assisted parallel syntheses of N‐substituted 1,4‐dihydropyridines of biological interest
Authors: Kumar B. R. Prashantha , Masih Pankaj , Lukose Chris Raju , Abraham Nimmy , Priya D. , Xavier Rinu Mary , Saji Kesiya , Adhikary Laxmi 
Published: April 2009

Abstract
magnified imageA solventless parallel synthesis of some novel, N‐substituted dihydropyridines by the application of microwaves is reported. The synthetic methodology adopted to synthesize the titled compounds and their antioxidant activity is described. J. Heterocyclic Chem., 46, 336 (2009).
Microwave‐assisted, solvent‐free, parallel syntheses and elucidation of reaction mechanism for the formation of some novel tetraaryl imidazoles of biological interest
Authors: Kumar B. R. Prashantha , Sharma Gyanendra Kumar , Srinath S. , Noor Mohamed , Suresh B. , Srinivasa B. R. 
Published: April 2009

Abstract
The microwave assisted, solvent free, parallel syntheses of title compounds is described in this protocol. Twelve new tetraaryl imidazoles, which are incorporated with the chemotherapeutic pharmacophores, have been synthesized by adopting one pot multicomponent reaction. Attempt has been made to investigate the mechanism behind the formation of tetraaryl imidazoles by product identification method. The synthesized compounds were analyzed by physical and analytical data. The synthesized compounds were evaluated for their antibacterial, antitubercular, and short‐term anticancer activity. Compound 13 was found to be the candidate compound to investigate further for its potential anticancer activity. J. Heterocyclic Chem., 46, 278 (2009).
Symmetrical diamides based on 2,6‐bis(methoxycarbonyl)pyridine: Syntheses and metal ion binding studies
Authors: Napitupulu Mery , Griggs Brendan L. , Luo Shi‐Xia , Turner Peter , Maeder Marcel , Lawrance Geoffrey A. 
Published: April 2009

Abstract
magnified imageSymmetrically‐armed molecules based on a 2,6‐diamidopyridine core, 2,6‐bis[N‐(1′‐piperidinylethyl)carbamyl]pyridine (1), 2,6‐bis[N‐(1′‐piperazinylethyl)carbamyl]pyridine (2), 2,6‐bis[N‐2′‐(5′′‐nitropyridine)‐1′‐azapropyl)carbamyl]pyridine (3), 2,6‐bis[N‐(3′‐hydroxypropyl)carbamyl]pyridine (4), 2,6‐bis[N‐(5′‐hydroxy‐3′‐azapentyl)carbamyl]pyridine (5), 2,6‐bis[N‐2′‐oxo‐2′,3′‐dihydropyrimidin‐4′‐yl)carbamyl]‐pyridine (6) as well as the thioamide analogue 2,6‐bis[(S‐2′‐pyridinyl)carbothiyl]pyridine (7) have been prepared and characterized. An X‐ray crystal structure of 1 confirms its formation. These molecules are potentially multidentate ligands for metal ions, and complexation has been probed through spectroscopic characterization, particularly by electrospray ionization mass spectrometry, and isolation of some first‐row transition metal complexes. The ligands, with potentially coordinating arms extending from each amide, dominantly form simple 1:1 M:L complexes. They show no tendency toward polynuclear helicate formation. J. Heterocyclic Chem., 46, 243 (2009).
Reaction of 2‐[(2‐Oxo‐2‐arylethyl)anilino]‐1‐aryl‐1‐ethanones with o‐phenylenediamine: Formation of quinoxalines
Authors: Ravindran Gurusamy , Muthusubramanian Shanmugam , Perumal Subbu 
Published: April 2009

Abstract
magnified imageUnexpected quinoxalines (3) have been obtained from a one‐pot reaction of 2‐[(2‐oxo‐2‐arylethyl)anilino]‐1‐aryl‐1‐ethanones (1) with o‐phenylenediamine (2) in the presence of a catalytic amount of p‐toluene sulfonic acid. The reaction presumably involves a tandem carbonyl addition—eliminative cyclization—air oxidation sequence. J. Heterocyclic Chem., 46, 332 (2009).
Investigation on the reactions of o‐hydroxybenzyl alcohols with vinyl ethers under acidic and/or thermal conditions
Authors: Kumbaraci Volkan , Ergunes Duygu , Midilli Melike , Begen Seckin , Talinli Naciye 
Published: April 2009

Abstract
magnified imageThe reaction of vinyl ethers with o‐hydroxybenzyl alcohols under different reaction conditions was investigated. The aim of this attempt was to find out whether the protection reactions or the hetero Diels–Alder reaction of quinone methide in situ generated from o‐hydroxybenzyl alcohol is more likely to occur. o‐hydroxybenzyl alcohols can give hetero Diels–Alder reactions with dihydro‐2H‐pyran at high temperatures but only when used with acid catalysts. At room temperature, even in the presence of acid catalyst, reactions yielded regular protection products. However, butyl vinyl ether and 4‐methoxy‐3‐butenone could not give intermolecular cycloaddition reactions under the acidic conditions, because both decomposed to the new products with acids. Hetero‐Diels–Alder products obtained only under thermal conditions but in low yields. J. Heterocyclic Chem., 46, 226 (2009).
A simple and novel eco‐friendly process for the synthesis of cyclic dithiocarbonates from epoxides and carbon disulfide in water
Authors: Halimehjani Azim Ziyaei , Ebrahimi Forogh , Azizi Najmedin , Saidi Mohammad R. 
Published: April 2009

Abstract
magnified imageThe reaction of oxiranes with carbon disulfide for preparation of cyclic dithiocarbonates was carried out in water under catalytic amount of an organic base such as dimethylaminopyridine or triethylamine. The reaction conditions are simple and give high yields of desired products. J. Heterocyclic Chem., 46, 347 (2009).
A novel method for the synthesis of 1(2H)‐phthalazinones using silica‐supported perchloric acid
Authors: Heravi Majid M. , Baghernejad Bita , Oskooie Hossein A. 
Published: April 2009

Abstract
magnified imageA simple and efficient synthesis of 1(2H)‐phthalazinone derivatives was achieved via reaction of phthalaldehydic acid and various phenyl hydrazines in acetonitrile using HClO4‐SiO2 as a catalyst in very good yields. J. Heterocyclic Chem., 46, 351 (2009).
Synthesis of pyrone carbaldehydes, pyrone sulfonium ylides and related epoxides
Authors: Shahrisa Aziz , Saraei Mahnaz 
Published: April 2009

Abstract
magnified imageHexaminium salts of 4‐pyrones 3a, 3b were synthesized by treatment of 2‐(4‐bromomethylphenyl)‐6‐methyl‐4H‐pyran‐4‐one 2a and 2‐(4‐bromomethylphenyl)‐6‐phenyl‐4H‐pyran‐4‐one 2b with hexamethyl‐enetetramine in chloroform in 71 and 84% yields respectively. Hydrolysis of 3a and 3b in EtOH:H2O produced the corresponding aldehydes 4a and 4b in 43 and 58% yields respectively. The reaction of bromopyrones 2b and 2c with dimethylsulfide in MeOH: CH2Cl2 afforded the corresponding sulfonium salts 5b and 5c in 66 and 65% yields respectively. Treatment of 5b and 5c with arene carbaldehydes such as (Ar = p‐ClC6H4, o‐ClC6H4, p‐NO2C6H4, o‐NO2C6H4, p‐FC6H4, 2‐naphthyl, p‐MeOC6H4, C6H5CHCH, p‐MeC6H4, C6H5 and 4‐(4‐oxo‐6‐phenyl‐4H‐pyran‐2‐yl)‐benzaldehyde 4b) in the presence of sodium hydroxide in CH3CN:H2O afforded eleven trans‐epoxides in 61–93% yields. J. Heterocyclic Chem., 46, 268 (2009).
New dihydro‐1,2,4‐triazolo[1,5‐a]pyrimidines based on arylidene derivatives of 5‐acetylbarbituric and dehydroacetic acids
Authors: Rudenko Roman V. , Komykhov Sergey A. , Musatov Vladimir I. , Desenko Sergey M. 
Published: April 2009

Abstract
magnified imageThe reaction of 3‐amino‐1,2,4‐triazole (1) with arylidene‐5‐acetyl barbituric acid (2b, 2c) or dehydroacetic acid (2a) by refluxing in butanol leads to the formation of dihydro‐1,2,4‐triazolo[1,5‐a]pyrimidines 3a, 3b, 3c. J. Heterocyclic Chem., 46, 285 (2009).
Isolated limb infusion: A review
Authors: Kroon Hidde M. , Thompson John F. 
Published: April 2009

AbstractIsolated limb perfusion is the preferred treatment option for locally advanced melanoma and sarcoma confined to a limb. This treatment results in high response rates with a satisfying duration of response in both tumours. A drawback of isolated limb perfusion, however, is the invasive and complex character of the procedure.Isolated limb infusion has been designed as a minimally invasive alternative to isolated limb perfusion. Treatment results of this simple technique, reported by various centres worldwide, show comparable response rates for melanoma and sarcoma. Therefore isolated limb infusion may replace isolated limb perfusion in the future as the preferred treatment option for these locally advanced limb tumours. J. Surg. Oncol. 2009;100:169–177. © 2009 Wiley‐Liss, Inc.