bluuebunny/crossref_metadata_2025_split huggingface dataset:
The crystal structure of Haloferax volcanii proliferating cell nuclear antigen reveals unique surface charge characteristics due to halophilic adaptation
Authors: Winter Jody A , Christofi Panayiotis , Morroll Shaun , Bunting Karen A 
Published: August 2009

Abstract

Background
The high intracellular salt concentration required to maintain a halophilic lifestyle poses challenges to haloarchaeal proteins that must stay soluble, stable and functional in this extreme environment. Proliferating cell nuclear antigen (PCNA) is a fundamental protein involved in maintaining genome integrity, with roles in both DNA replication and repair. To investigate the halophilic adaptation of such a key protein we have crystallised and solved the structure of Haloferax volcanii PCNA (Hv PCNA) to a resolution of 2.0 Å.

Results
The overall architecture of Hv PCNA is very similar to other known PCNAs, which are highly structurally conserved. Three commonly observed adaptations in halophilic proteins are higher surface acidity, bound ions and increased numbers of intermolecular ion pairs (in oligomeric proteins). Hv PCNA possesses the former two adaptations but not the latter, despite functioning as a homotrimer. Strikingly, the positive surface charge considered key to PCNA's role as a sliding clamp is dramatically reduced in the halophilic protein. Instead, bound cations within the solvation shell of Hv PCNA may permit sliding along negatively charged DNA by reducing electrostatic repulsion effects.

Conclusion
The extent to which individual proteins adapt to halophilic conditions varies, presumably due to their diverse characteristics and roles within the cell. The number of ion pairs observed in the Hv PCNA monomer-monomer interface was unexpectedly low. This may reflect the fact that the trimer is intrinsically stable over a wide range of salt concentrations and therefore additional modifications for trimer maintenance in high salt conditions are not required. Halophilic proteins frequently bind anions and cations and in Hv PCNA cation binding may compensate for the remarkable reduction in positive charge in the pore region, to facilitate functional interactions with DNA. In this way, Hv PCNA may harness its environment as opposed to simply surviving in extreme halophilic conditions.
Modulation of cytokines and transcription factors (T-Bet and GATA3) in CD4 enriched cervical cells of Chlamydia trachomatis infected fertile and infertile women upon stimulation with chlamydial inclusion membrane proteins B and C
Authors: Gupta Rishein , Vardhan Harsh , Srivastava Pragya , Salhan Sudha , Mittal Aruna 
Published: August 2009

AbstractBackgroundChlamydial Inclusion membrane proteins (Incs), are involved in biochemical interactions with host cells and infecting Chlamydiae. We have previously reported the role of two Chlamydia trachomatis (CT) Incs, namely IncB and IncC in generating host immunity in CT infected women. Emerging data shows involvement of Inc stimulated CD4 positive T cells in aiding host immunity in infected fertile and infertile women through the secretion of interferon gamma. However the lack of data on the intra-cytokine interplay to these Incs in infected cell milieu prompted us to investigate further.MethodsA total of 14 CT-positive fertile, 18 CT-positive infertile women and 25 uninfected controls were enrolled in this study. CD8 depleted, CD4 enriched cervical cells were isolated and upon stimulation with IncB and IncC, modulation of cytokines (Interleukin (IL)-1 Beta, IL-4, IL-5, IL-6, IL-10, Interferon-gamma, IL-12, IL-23, Tumor Necrosis Factor-alpha and Granulocyte macrophage colony-stimulating factor (GM-CSF) and T cell lineage regulating transcription factors T-Bet and GATA3 was determined by real-time reverse-transcriptase (RT)-PCR and ELISA.ResultsSignificant higher expression (P < 0.05) of Interferon-gamma, IL-12, IL-23 and GM-CSF were found in Inc-stimulated CD4 enriched cervical cells of CT-positive fertile women and contrastingly high IL-1 Beta, IL-4, IL-5, IL-6 and IL-10 levels were found in CT-positive infertile women. Positive correlation (P < 0.05) was found between Interferon-gamma and T-Bet levels in CT-positive fertile women and IL-4 mRNA and GATA3 levels in CT-positive infertile patients upon IncB and IncC stimulation.ConclusionOverall our data shows that CT IncB and IncC are able to upregulate expression of cytokines, namely interferon-gamma, IL-12, IL-23 and GM-CSF in CT-positive fertile women while expression of IL-1 Beta, IL-4, IL-5, IL-6 and IL-10 were upregulated in CT-positive infertile women. Our study also suggests that Incs are able to modulate expression of T cell lineage determinants indicating their involvement in regulation of immune cells.
Comparison of the Performances of Wild Anastrepha obliqua (Diptera: Tephritidae) Individuals Proceeding From Different Hosts
Authors: Medeiros-Santana Luanda , Zucoloto Fernando S 
Published: August 2009

Abstract
Feeding during the insect immature phase is extremely important for an adequate performance in adult life. The nutritional value of the ingested diet during the immature phase directly affects the percentage of emergence, the duration of the immature phase, and the size of the emerging adults. The objectives of this study were to compare the performance of adult Anastrepha obliqua (Macquart) (Diptera: Tephritidae) females, the pupae per kg of fruit, and the percentage of emergence of flies that spent the larval phase in three fruit species (Mangifera indica L., Spondias lutea L., and S. venulosa L.), taking into account the amount of nutrients in the fruit. Weekly collections were carried out during the fructification period of the hosts. Adult size, longevity, egg production, ingestion of artificial diets by adult females, number of pupae per fruit and pupae per kg of fruit, and percentage of adult emergence were determined. The results showed that S. venulosa was the main A. obliqua host and the most infested among the studied fruit. The adult performance did not vary among the studied hosts; however, the percentage of emergence did. The amount of nutrients in the fruit did not affect the parameters that were analyzed. It is possible that the physical characteristics of the fruit were more important to wild A. obliqua females in their choice for an oviposition site than the amount of nutrients per se. Furthermore, the fruit surface/volume ratio also was involved in the infestation index.
Can Marketing Resources Contribute to Company Performance?
Authors: Milfelner Borut , Gabrijan Vladimir , Snoj Boris 
Published: August 2009

Can Marketing Resources Contribute to Company Performance?
This study investigates the relationships between market orientation, innovation resources, reputational resources, customer related capabilities and distribution-based assets, as well as their impact on both market and financial performance. The results indicate that market orientation is indirectly related to a company's market and financial performance through the four other marketing resources. Reputational resources have a positive impact on loyalty, market share and sales volume, while the impact of innovation resources on the market share and sales volume is more indirect and through customer loyalty. While customer-related capabilities significantly impact customer loyalty, their impact on the market share and sales volume can not be confirmed. On the other hand, the distribution-based assets are only weakly related to loyalty, the market share and the sales volume. The general findings indicate that selected marketing resources impact financial performance indirectly through the creation of customer loyalty and directly through the market share and sales volume.
The Job Satisfaction of Detectives Investigating Economic Crime in the Police Directorates of Ljubljana-Maribor and Some Reasons for their Fluctuation
Authors: Gorenak Vinko , Željko Božidar 
Published: August 2009

The Job Satisfaction of Detectives Investigating Economic Crime in the Police Directorates of Ljubljana-Maribor and Some Reasons for their Fluctuation
An average person spends almost a third of his life at work. It is therefore very important that the person is satisfied with their job. We could define job satisfaction as a factor with an important influence on increased quality in both professional and private life. The dissatisfaction of employees with their jobs is usually followed by fluctuation. This can be very damaging to an organization since employees are one of the most important resources.
This article presents the results of various surveys on the satisfaction of police officers and detectives with their work, on fluctuation as a phenomenon and some of the reasons for it.
In the empirical part of this article, the authors present a study done among the economic crime detectives working in the police directorates of Ljubljana and Maribor. The study showed that economic crime detectives are somewhat satisfied with their work but are still willing to change their jobs and leave the police for a better salary. The main factor for dissatisfaction is the low pay for their performance.
The Management of Complaints in Slovene Public Administration
Authors: Stare Janez , Gruden Barbara , Kovač Polonca 
Published: August 2009

The Management of Complaints in Slovene Public Administration
The Slovene public administration is part of the broader social system, therefore it must be responsive and proactive. The instrument of complaint in the administrative procedure, and wider in the context of the entire administrative management, is very helpful to public administration for tracking social changes and should therefore be seen as a form of constructive criticism. On the basis of user complaints regarding its services or the parties to the administrative procedure, the public administration must learn permanently and, as learning organisation, must incorporate its findings into future practice. The starting point of this contribution is the problems of the complaint in the narrow sense - customer dissatisfaction with the functioning of the public administration or arising from the fact that one of the characteristics of the administrative procedure or other services is the direct contact between the customer and the provider of the service. With this approach, the public administration will develop part of an integral system of quality and excellence that underlines the meaning of satisfaction of (all) the users of public services.
The Role of Information for Recognising Business Opportunities
Authors: Skerbinjek Andreja 
Published: August 2009

The Role of Information for Recognising Business Opportunities
Managers need a lot of knowledge and information to make decisions and recognize business opportunities. They can acquire this knowledge and information in different ways. Accounting information, particularly information relating to the creditworthiness of business partners and competitors, is important. Such information, which can contain non-accounting information, is often derived from annual reports. The fastest and cheapest way of accessing information is via the Internet. Because searching for information about different businesses on their Web sites can be time consuming, a quicker, more efficient option is to access this information on databases that contain useful information relating to the majority of businesses in the country. In this paper, we discuss the importance of knowledge and information for recognizing business opportunities. We also analyze the appropriateness of accounting information available from five of the most widely used databases concerning Slovene and Croatian businesses, for estimating the creditworthiness of businesses.
Organisational Effectiveness and Customer Satisfaction
Authors: Ambrož Milan , Praprotnik Martina 
Published: August 2009

Organisational Effectiveness and Customer Satisfaction
This paper presents a test of the relationship between organizational culture as a crucial indicator of organizational effectiveness and customer satisfaction using service-unit data from two health resorts. Ensuring survival of the service organisation in the long run requires adaptations which are oriented towards achieving maximum customer satisfaction. This study intended to unveil the effect organisational factors have on customer service orientation from the customer and employee point of view within a two health resort service setting. The finding suggests that when trying to predict the comparative degree which organisational effectiveness factors have in satisfying customers' needs, performance, adaptability and mission can be of the highest importance. Some effects like performance were uniform for employees and customers, while others varied depending on the organisation and the customer or employee group. Furthermore, findings suggest that service performance and organisation mission of the service organisation predict customer satisfaction based on established and proven health services. In this context there is no room for innovation, despite the fact that employees and customers do not share similar views about the impact of organisational effectiveness. Developing an effective service organisation can provide a competitive advantage to the organisation. Critical for the success of the service organisation is that organisational agents have a clear view of the existing organisation effectiveness and a clear view of the customer expectations in this area.
E-education between Pedagogical and Didactic Theory and Practice
Authors: Rebolj Vanda 
Published: August 2009

E-education between Pedagogical and Didactic Theory and Practice
Education systems confront new requirements to become more efficient and contribute more to the economic success of society. At the same time there are new challenges for the education systems to perform their tasks using advanced technology. In capacity, information technology considerably surpasses the current learning technology. Supported by Internet it creates an electronic parallel to the standard school and learning. Unfortunately there is only sporadic evidence that supported by information technology, learning and education system as a whole can be considerably improved. In spite of that, we believe there is no way back and e-education is not just a temporary whim. Even though we do not know exactly who should perform what and to which scope. The passiveness of pedagogues, the lack of empirical research and the occurring expansion of the market for e-education products without their quality assessment is not of advantage to this progress. The spread of e-education production without considering the professional opinion of pedagogues, who beside their practical work must also engage in developing their own pedagogical theory, might not benefit the existing education achievements. On the other hand, didactics can by analogy be applied also in the web environment, consequently creating the related assessment mechanisms of the electronic education elements.
Organisational Culture as Organisational Identity — Between the Public and the Private
Authors: Erčulj Justina 
Published: August 2009

Organisational Culture as Organisational Identity — Between the Public and the Private
The author discusses the notion of organisational culture and relates it to the notion of organisational identity. Culture as a group identity involves elements of stability, homogeneity and integrity but this can be very much altered through post-modern understanding of relationships between localities and their contexts. The research that was conducted in two primary schools in Slovenia is based on the symbolic notion of organisational culture. We explored what meanings are assigned to schools in public documents, during rituals and in teachers' and headteachers'narratives. The findings indicate the co-existence of two cultures and hence of two identities, ‘the public’ and ‘the private’, the former being closer to the corporate identity and the latter to multiple identities. The managers should be aware of both and should use the in-between space for on-going discussions and negotiations.
Managers and Leaders in Organizations of a Post-transition Economy
Authors: Prevodnik Mojca , Biloslavo Roberto 
Published: August 2009

Managers and Leaders in Organizations of a Post-transition Economy
Managers and leaders are different figures within an organization but are also alike. During different stages of the organizational life cycle we sometimes need more manager-oriented people and at other times more leader-oriented people. However most of the time we need them both in order to achieve a balance within the organization. Slovenia is a post-transition economy, facing new challenges and opportunities therefore the need for leader-oriented people is expected to be huge. Nevertheless our research showed that in Slovenian profit and non-profit organizations, upper management positions have been occupied by manager-oriented people but at the same time a balance exists between the two roles. We also found out that managers and leaders perceive the success of their organisations in quite a similar way. An adapted Hickman's questionnaire together with a content analysis of subordinates' comments about how they perceive their organization was used to ascertain the differences between them and their superiors. By knowing how employees perceive their managers and how the latter perceive themselves and their organizations we can develop better approaches to management development in post-transition economies.
The Salary System in the Private Sector in the Republic Slovenia
Authors: Korpič-Horvat Etelka , Tičar Bojan , Bohinc Rado 
Published: August 2009

The Salary System in the Private Sector in the Republic Slovenia
In the article the authors discuss the salary system in the private sector in the Republic of Slovenia. They present relevant legislation dealing with remuneration, in particular the Employment Relations Act, which regulates individual employment relations and in a separate chapter regulates remuneration. Remuneration includes a salary and other additional payments. The authors treat all the components of a salary, i.e. the basic salary, extra payments, the part of the salary based on job performance, and the payment for positive business performance. Other additional payments are payments in kind or in money, in securities, or profit-related pay, i.e. the participation of employees in profit sharing. The authors also treat other income of employees laid down in the Employment Relations Act and collective agreements as well as the reimbursement of expenses related to work. The authors also focus on the obligatory content of an employment contract with reference to remuneration. Finally, they address open issues regarding types of other additional payments and possible solutions for determining individual remuneration instruments in collective agreements and in employment contracts.
The Centrohelid Heliozoans (Protista, Centrohelida) of Algal Mats from the Water Body in Ukrainian Polissya
Authors: Gaponova L. 
Published: August 2009

The Centrohelid Heliozoans (Protista, Centrohelida) of Algal Mats from the Water Body in Ukrainian PolissyaPterocystis foliacea (Du#rrschmidt, 1985), Raineriophrys erinaceoides (Petersen et Hansen, 1960) and Acanthocystis dresscheri Siemensma et Roijackers, 1988 were observed in algal mats from the pond. All the three species are redescribed.
Four New Species of Mites of The Genus Bakerdania (Acari, Heterostigmata, Pygmephoroidea) From Crimea
Authors: Khaustov A. 
Published: August 2009

Four New Species of Mites of The Genus Bakerdania (Acari, Heterostigmata, Pygmephoroidea) From Crimea
Four new species of mites of the genus Bakerdania Sasa, 1961: B. cavernicola Khaustov, sp. n., B. taurica Khaustov, sp. n., B. brevicaudata Khaustov, sp. n., and B. yaltaensis Khaustov, sp. n. are described from soils in Crimea.
The Community of Strongylids (Nematoda, Strongylida) of Working Donkeys (Equus Asinus) in Ukraine
Authors: Kuzmina T. , Kuzmin Yu. 
Published: August 2009

The Community of Strongylids (Nematoda, Strongylida) of Working Donkeys (Equus Asinus) in UkraineThe aim of our work was to study the species composition of the strongylid community of donkeys and to explore the influence of anthelmintic treatments on the community structure. Strongylid nematodes were collected by the diagnostic deworming technique from 33 donkeys from the riding school "Chudo-oslik" (Crimea) (25), from Kharkiv (3) and Kyiv zoos (2) and Kyiv riding schools (3). Seventeen species were found in donkeys studied: 16 species of Cyathostominae and 1 of Strongylinae. Between 2 and 7 species were found per donkey (average of 4.2 ± 2.8). Cyathostomum tetracanthum, C. catinatum, Cylicocyclus nassatus, Cylicostephanus goldi and C. longibursatus) dominated in the community; they were found in 80-100% animals studied and comprised 91.7% of the total number of strongylids collected. Two species C. tetracanthum and Cylicocyclus auriculatus were found to be specific for donkeys. The results obtained showed a reduction of the species richness of the strongylid community in donkeys from riding schools and zoos caused by lack of grazing and by regular anthelmintic treatments.
A Review of Palearctic Species of the Genus Pseuderimerus (Hymenoptera, Torymidae) with a Description of One New Species From Iran
Authors: Zerova M. , Seryogina L. 
Published: August 2009

A Review of Palearctic Species of the Genus Pseuderimerus (Hymenoptera, Torymidae) with a Description of One New Species From Iran
The Genus Pseuderimerus includes 9 species in the World fauna, six of them are Palaearctic. Pseuderimerus irani Zerova et Seryogina, sp. n. is described. A key to all palaearctic species is given. The holotype and paratypes of the new species are deposited in the collection of the Institute of Zoology, National Academy of Sciences of Ukraine (Kyiv).
Rhabdias Pearsoni SP. N. (Nematoda, Rhabdiasidae) From Keelback, Tropidonophis Mairii (Reptilia, Colubridae) In Australia
Authors: Kuzmin Yu. , Tkach V. 
Published: August 2009

Rhabdias PearsoniSP. N. (Nematoda, Rhabdiasidae) From Keelback,Tropidonophis Mairii(Reptilia, Colubridae) In AustraliaRhabdias pearsoniKuzmin et Tkach, sp. n. is described based on specimens found in the lung of the keelbackTropidonophis mairii(Gray, 1841) from southern Queensland, Australia. The new species is most similar morphologically toRhabdias horigutiiYamaguti, 1943 andRhabdias vibakariKuzmin, 1999 with all three species lacking buccal capsule. The new species differs fromR. horigutiiby the smaller circumoral lips and papillae, and shorter tail.R. pearsonisp. n. differs fromR. vibakariby position of the nerve ring (inR. vibakari, it is closer to the anterior end than in the new species) and shorter tail. AustralianR. pearsonisp. n. is geographically isolated fromR. horigutiiand R. vibakari, both occurring in Eastern Asia.
Synonymy of the Genera Pseudobakerdania and Ultrasiteroptes (Acari, Heterostigmata, Pygmephoridae) with First Record and Redescription of Ultrasiteroptes Adamisi From Ukraine
Authors: Khaustov A. 
Published: August 2009

Synonymy of the GeneraPseudobakerdaniaandUltrasiteroptes(Acari, Heterostigmata, Pygmephoridae) with First Record and Redescription ofUltrasiteroptesAdamisi From UkraineThe genus namePseudobakerdaniaKaliszewski, 1988 is shown as junior subjective synonym of genusUltrasiteroptesLivshits, Mitrofanov et Sharonov, 1986.Ultrasiteroptes adamisi(Mahunka, 1968) comb. n. is redescribed and recorded for the first time from Ukraine.