bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysing the cost impact of failure rates for the next generation of offshore wind turbines
Authors: Donnelly Orla , Carroll James , Howland Michael 
Published: May 2024

AbstractOffshore wind turbines have rapidly scaled up in recent years, with plans to construct turbines up to 22 MW in the next decade. However, the operations and maintenance (O&M) requirements for these ‘next‐generation turbines’ remain largely unknown. In this study, the total O&M costs are calculated, using a bench‐marked O&M model, for a hypothetical 10 MW turbine scenario using two drive train configurations, based on known failure rates of smaller turbines. The O&M costs of the 10 MW turbines are compared with those of existing 3 MW turbines in two case studies: a North Sea wind farm and an East Coast US wind farm. Overall, direct drive 10 MW turbines performed better depending on the site's climate conditions. The study indicated that the two‐stage drive train configuration may be more suitable for the US site than the North Sea, depending on the turbine's failure rate. The US site benefited from increased availability due to more favourable weather windows, resulting in lower lost revenue for the two‐stage configuration despite high transport costs. The study found that the failure rate of 10 MW offshore wind turbines in the North Sea with a two‐stage gearbox can increase by as much as 30% compared to the 3 MW failure rates without increasing direct O&M costs. These findings are crucial for the offshore wind energy industry, particularly for OEMs, developers and maintenance providers, as they provide insights into the required reliability for next generation turbines to reduce O&M costs compared to existing 3 MW turbines.
A systematic review on microplastic contamination in marine Crustacea and Mollusca of Asia: Current scenario, concentration, characterization, polymeric risk assessment, and future Prospectives
Authors: Doshi Mahima , Rabari Vasantkumar , Patel Ashish , Yadav Virendra Kumar , Sahoo Dipak Kumar , Trivedi Jigneshkumar 
Published: May 2024

AbstractMicroplastics (MPs) pollution has wreaked havoc on biodiversity and food safety globally. The false ingestion of MPs causes harmful effects on organisms, resulting in a decline in biodiversity. The present review comprehended the current knowledge of MP contamination in Crustacea and Mollusca from 75 peer‐reviewed articles published in Asia between 2015 and 2023. A total of 79 species (27 Crustacea and 52 Mollusca) have been recorded to be contaminated with MPs. Out of the total 27 species of Crustacea, Metopograpsus quadridentatus (327.56 MPs/individual) and Balanus albicostatus (0.42 MPs/individual) showed the highest and lowest contamination, respectively. Out of the total 52 species of Mollusca, Dolabella auricularia (2325 MPs/individual) and Crassostrea gigas and Mytilus edulis (0.2 MPs/individual) showed the highest and lowest contamination, respectively. In terms of country‐wise MP contamination, China has the highest number of contaminated species in both phylums among Asia. Findings of pollution indices revealed a very high risk of MP contamination in all the countries. Fiber was reported predominantly in both groups. Blue and black‐colored MPs having <500 μm and <500 μm–1 mm size were found dominantly in Crustacea and Mollusca, respectively. Polypropylene was recorded as the dominant plastic polymer in both Crustacea and Mollusca. In essence, this review has provided a comprehensive insight into MP concentration in Crustacea and Mollusca of Asia, highlighting variations among species and geographic locations. This understanding is crucial for tackling urgent environmental challenges, safeguarding human health, and promoting global sustainability initiatives amid the escalating issue of plastic pollution.Practitioner Points

Microplastic pollution has created havoc on biodiversity and food safety.
A total of 27 and 52 species of crustaceans and Mollusca have been recorded to be contaminated with MPs.
Metopograpsus quadridentate and Dolabella auricularia have shown higher MPs contamination.
Polypropylene was recorded as the dominant plastic polymer in both crustacean and Mollusca.
Findings of pollution indices revealed a very high risk of MP contamination in all the countries.
New regulatory role of Znf1 in transcriptional control of pentose phosphate pathway and ATP synthesis for enhanced isobutanol and acid tolerance
Authors: Ali Syed Azhar , Songdech Pattanan , Samakkarn Wiwan , Duangphakdee Orawan , Soontorngun Nitnipa 
Published: May 2024

AbstractTo develop a cost‐effective microbial cell factory for the production of biofuels and biochemicals, an understanding of tolerant mechanisms is vital for the construction of robust host strains. Here, we characterized a new function of a key metabolic transcription factor named Znf1 and its involvement in stress response in Saccharomyces cerevisiae to enhance tolerance to advanced biofuel, isobutanol. RNA‐sequencing analysis of the wild‐type versus the znf1Δ deletion strains in glucose revealed a new role for transcription factor Znf1 in the pentose phosphate pathway (PPP) and energy generation. The gene expression analysis confirmed that isobutanol induces an adaptive cell response, resulting in activation of ATP1‐3 and COX6 expression. These genes were Znf1 targets that belong to the electron transport chain, important to produce ATPs. Znf1 also activated PPP genes, required for the generation of key amino acids, cellular metabolites, and maintenance of NADP/NADPH redox balance. In glucose, Znf1 also mediated the upregulation of valine biosynthetic genes of the Ehrlich pathway, namely ILV3, ILV5, and ARO10, associated with the generation of key intermediates for isobutanol production. Using S. cerevisiae knockout collection strains, cells with deleted transcriptional regulatory gene ZNF1 or its targets displayed hypersensitivity to isobutanol and acid inhibitors; in contrast, overexpression of ZNF1 enhanced cell survival. Thus, the transcription factor Znf1 functions in the maintenance of energy homeostasis and redox balance at various checkpoints of yeast metabolic pathways. It ensures the rapid unwiring of gene transcription in response to toxic products/by‐products generated during biofuel production. Importantly, we provide a new approach to enhance strain tolerance during the conversion of glucose to biofuels.
Synthesis and Structure of Heavy Alkali Metal Pentalenides
Authors: Sanderson Hugh J. , Banerjee Sumanta , Kaur Mandeep , Kennedy Alan R. , Kociok‐Köhn Gabriele , Hintermair Ulrich , Robertson Stuart D. 
Published: May 2024

AbstractThe solid‐state structures of the first rubidium and caesium pentalenides [Rb(THF)]2[Ph4Pn] and [Cs(THF)]2[Ph4Pn] have been determined by single crystal X‐ray diffraction. Both were found to be polymeric in the solid state through interactions of the cations with the phenyl substituents, in contrast to their lighter group 1 congeners which are monomeric for lithium and sodium, and THF‐bridged for potassium. Both [Rb(THF)]2[Ph4Pn] and [Cs(THF)]2[Ph4Pn] displayed increased η8 coordination, demonstrating a shift towards higher hapticities down the group as previously predicted computationally for the parent M2[Pn] complexes (M=group 1 metal). The solid‐state structures of the polydentate donor adducts [M(DME)x]2[Ph4Pn] (M=Li, x=1; M=Na, x=2) and [M(Me6TREN)]2[Ph4Pn] (M=K, Rb, Cs) were all monomeric and displayed increased metal‐carbon distances and decreased ring slippage values relative to the THF adducts.
Strengthening and precipitation hardening mechanisms of surface-mechanically treated 17-4PH stainless steel
Authors: Olugbade Temitope Olumide , Oladapo Bankole I. , Zhao Qi , Ting Tin Tin 
Published: May 2024

AbstractAchieving ultra-high strength without sacrificing too much ductility is the focus of attention in nanostructured materials. Here, the strengthening mechanism and property enhancement of surface-mechanically treated 17-4PH stainless steel (SS17-4PH) were investigated. Our findings show that a grain refinement and elongated lath-like martensitic grain (~ 50 nm thick) could be produced after surface treatment. The grain size remains in the nanoscale, and random crystallographic orientations with the presence of nanocrystallites characterize the nanocrystalline grains formed on the treated sample. This contributes to the property enhancement with a yield strength of about 901 MPa and a reduced elongation to failure of about 17%. The atom probe tomography (APT) characterization unveiled the emergence of high-density precipitate (Cu-rich) at the material surface, with a number density of about 2.6255 × 1024 m−3 and an average radius of 2.22 nm. Besides, the dislocation activities caused by SMAT result in the gradual breakdown of precipitates into smaller sizes and final dissolution in the matrix, increasing the number of nucleation sites and leading to more grain refinement processes. The grain boundary, dislocation densities, and the Cu-rich precipitate greatly influence the strengthening mechanism of surface-treated SS17-4PH.
Enhancing the expression of the unspecific peroxygenase in Komagataella phaffii through a combination strategy
Authors: Zhao Li-Xiang , Zou Shu-Ping , Shen Qi , Xue Ya-Ping , Zheng Yu-Guo 
Published: May 2024

Abstract
The unspecific peroxygenase (UPO) from Cyclocybe aegerita (AaeUPO) can selectively oxidize C–H bonds using hydrogen peroxide as an oxygen donor without cofactors, which has drawn significant industrial attention. Many studies have made efforts to enhance the overall activity of AaeUPO expressed in Komagataella phaffii by employing strategies such as enzyme-directed evolution, utilizing appropriate promoters, and screening secretion peptides. Building upon these previous studies, the objective of this study was to further enhance the expression of a mutant of AaeUPO with improved activity (PaDa-I) by increasing the gene copy number, co-expressing chaperones, and optimizing culture conditions. Our results demonstrated that a strain carrying approximately three copies of expression cassettes and co-expressing the protein disulfide isomerase showed an approximately 10.7-fold increase in volumetric enzyme activity, using the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as the substrate. After optimizing the culture conditions, the volumetric enzyme activity of this strain further increased by approximately 48.7%, reaching 117.3 U/mL. Additionally, the purified catalytic domain of PaDa-I displayed regioselective hydroxylation of R-2-phenoxypropionic acid. The results of this study may facilitate the industrial application of UPOs.

Key points
• The secretion of the catalytic domain of PaDa-I can be significantly enhanced through increasing gene copy numbers and co-expressing of protein disulfide isomerase.
• After optimizing the culture conditions, the volumetric enzyme activity can reach 117.3 U/mL, using the 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) as the substrate.
• The R-2-phenoxypropionic acid can undergo the specific hydroxylation reaction catalyzed by catalytic domain of PaDa-I, resulting in the formation of R-2-(4-hydroxyphenoxy)propionic acid.
New copula families and mixing properties
Authors: Longla Martial 
Published: May 2024

AbstractWe characterize absolutely continuous symmetric copulas with square integrable densities in this paper. This characterization is used to create new copula families, that are perturbations of the independence copula. The full study of mixing properties of Markov chains generated by these copula families is conducted. An extension that includes the Farlie–Gumbel–Morgenstern family of copulas is proposed. We propose some examples of copulas that generate non-mixing Markov chains, but whose convex combinations generate $$\psi $$
ψ
-mixing Markov chains. Some general results on $$\psi $$
ψ
-mixing are given. The Spearman’s correlation $$\rho _S$$

ρ
S

and Kendall’s $$\tau $$
τ
are provided for the created copula families. Some general remarks are provided for $$\rho _S$$

ρ
S

and $$\tau $$
τ
. A central limit theorem is provided for parameter estimators in one example. A simulation study is conducted to support derived asymptotic distributions for some examples.
Winter “warm Arctic-cold Eurasia” pattern and its statistical linkages to oceanic precursors during the era of satellite observations
Authors: Schlichtholz Pawel 
Published: May 2024

AbstractA striking recurrent feature of winter climate variability is the “warm Arctic-cold Eurasia” (WACE) pattern of opposite sign anomalies of surface air temperature (SAT) in the Barents Sea region and midlatitude Eurasia. Its origins and mechanisms are hotly debated, and its predictability remains unknown. This study investigates statistical relationships of the winter WACE dipole with concurrent anomalies of atmospheric circulation and oceanic precursors during the era of satellite observations. The results reveal a high potential for seasonal predictability of not only the WACE dipole but also several related indicators of winter climate variability, including the Arctic and Eurasian SAT anomalies. During subperiods of extreme covariability between the Arctic and Eurasian SATs around the early 1980s and late 2000s, most of the WACE variability is explained by ocean temperature and surface turbulent heat flux anomalies in the Barents Sea region during the preceding months. Anomalies in summer Atlantic water temperature (AWT) and autumnal sea surface temperature (SST) in this region explain about 70–80% of the variance of the following winter WACE variability during all events of strong Arctic-Eurasian SAT covariability. Analysis of SST variability in the Arctic-North Atlantic region suggests that the winter WACE link to the summer AWT anomalies reflects an atmospheric response to a large-scale surface reemergence of ocean temperature anomalies. However, this linkage had been robust only until the early 2000s. Since then, the winter WACE variability has been strongly related to autumnal SST anomalies in the Barents Sea region and the North Pacific.
Rise in complications of acute otitis media during and after the COVID-19 pandemic
Authors: Hollborn Hannes , Lachmann Christoph , Strüder Daniel , van Bonn Sara M. , Mlynski Robert , Schraven Sebastian P. 
Published: May 2024

Abstract
Purpose
After the lifting of nonpharmaceutical interventions (NPIs) during the COVID-19 pandemic, clinical observation showed an increase in complications of acute otitis, followed by a rise in the number of mastoidectomies performed. The aim of this study was to record the number of mastoidectomies performed before, during and after the COVID-19 pandemic as an indicator for complications of acute otitis media.

Methods
Data were collected from a tertiary hospital in a university setting, as well as from four major public health insurance companies in Germany. The data of 24,824,763 German citizens during a period from 2014 until 2023 were analyzed.

Results
According to the data, during the COVID-19 pandemic, the number of mastoidectomies performed dropped by 54% for children aged 0–6 and by 62% for children aged 7–18. For adults, there were 30% fewer mastoidectomies performed between 2020 and 2022. After the lifting of most NPI’s in the season from July 2022 to June 2023, there was a sharp increase in the number of mastoidectomies performed on patients of all ages.

Conclusions
During the COVID-19 pandemic, a decrease in the number of mastoidectomies performed was seen, suggesting a lower incidence of complicated acute otitis, most likely linked to the general decrease of upper airway infections due to NPI’s. In contrast, a sharp increase in the incidence of complicated otitis occurred after the hygiene measures were lifted. The current development causes a more frequent performance of mastoidectomies, thus entailing a change in the challenges for everyday clinical practice.
Transanal ileal pouch-anal anastomosis: A systematic review and meta-analysis of technical approaches and clinical outcomes
Authors: Stephens Ian J. B. , Byrnes Kevin G. , Burke John P. 
Published: May 2024

Abstract
Purpose
Transanal minimally invasive surgery has theoretical advantages for ileal pouch-anal anastomosis surgery. We performed a systematic review assessing technical approaches to transanal IPAA (Ta-IPAA) and meta-analysis comparing outcomes to transabdominal (abd-IPAA) approaches.

Methods
Three databases were searched for articles investigating Ta-IPAA outcomes. Primary outcome was anastomotic leak rate. Secondary outcomes included conversion rate, post operative morbidity, and length of stay (LoS). Staging, plane of dissection, anastomosis, extraction site, operative time, and functional outcomes were also assessed.

Results
Searches identified 13 studies with 404 unique Ta-IPAA and 563 abd-IPAA patients. Anastomotic leak rates were 6.3% and 8.4% (RD 0, 95% CI -0.066 to 0.065, p = 0.989) and conversion rates 2.5% and 12.5% (RD -0.106, 95% CI -0.155 to -0.057, p = 0.104) for Ta-IPAA and abd-IPAA. Average LoS was one day shorter (MD -1, 95% CI -1.876 to 0.302, p = 0.007). A three-stage approach was most common (47.6%), operative time was 261(± 60) mins, and total mesorectal excision and close rectal dissection were equally used (49.5% vs 50.5%). Functional outcomes were similar. Lack of randomised control trials, case-matched series, and significant study heterogeneity limited analysis, resulting in low to very low certainty of evidence.

Conclusions
Analysis demonstrated the feasibility and safety of Ta-IPAA with reduced LoS, trend towards less conversions, and comparable anastomotic leak rates and post operative morbidity. Though results are encouraging, they need to be interpreted with heterogeneity and selection bias in mind. Robust randomised clinical trials are warranted to adequately compare ta-IPAA to transabdominal approaches.
Fecal calprotectin and platelet count predict histologic disease activity in pediatric ulcerative colitis: results from a projection-predictive feature selection
Authors: Schiller B. , Wirthgen E. , Weber F. , Schiller S. , Radke M. , Claßen M. , Däbritz J. ,   CEDATA-GPGE Study Group, Buderus S. , Bufler P. , Däbritz J. , Dammann S. , de Laffolie J. , Friedt M. , Hauer A. , Keller K. M. , Krahl A. , Laaß M. , Lang T. , Posovszky C. , Rodeck B. , Trenkel S. 
Published: May 2024

AbstractEspecially for pediatric patients, proxies of mucosal inflammation are needed. The Pediatric Ulcerative Colitis Activity Index (PUCAI) has been established to predict clinical and endoscopic disease activity. However, histologic inflammation might persist. We applied a special variable selection technique to predict histologic healing in pediatric ulcerative colitis (UC) as parsimoniously (but still as precisely) as possible. The retrospective analysis included data from two study cohorts, comprising 91 visits from 59 pediatric patients with UC. A Bayesian ordinal regression model was used in combination with a projection-predictive feature selection (PPFS) to identify a minimal subset of clinical and laboratory parameters sufficient for the prediction of histologic disease activity. Following the PPFS, CEDATA-GPGE patient registry data were analyzed to investigate the relevance of the selected predictors in relation to PUCAI and Physician Global Assessment (PGA) in up to 6697 patient visits. Fecal calprotectin (FC) and platelet count were identified as the minimal subset of predictors sufficient for prediction of histologic disease activity in pediatric UC. FC and platelet count also appeared to be associated with increasing disease activity as measured by PUCAI and PGA in the CEDATA-GPGE registry. Based on the selected model, predictions can be performed with a Shiny web app. Conclusion: Our statistical approach constitutes a reproducible and objective tool to select a minimal subset of the most informative parameters to predict histologic inflammation in pediatric UC. A Shiny app shows how physicians may predict the histologic activity in a user-friendly way using FC and platelet count. To generalize the findings, further prospective studies will be needed.

What is Known:• Histologic healing is a major endpoint in the therapy of ulcerative colitis (UC).• The PUCAI score has been established to predict disease activity in pediatric UC but is not suitable for the prediction of histologic healing.

What is New:• Our Bayesian ordinal regression model in combination with a projection-predictive feature selection is a reproducible and objective tool to select the minimal subset of clinical and laboratory parameters to predict histologic inflammation in pediatric UC.• Histologic inflammation in pediatric UC can be non-invasively predicted based on the combination of fecal calprotectin levels and platelet count.
Functional similarity affects similarity in partner composition in flea-mammal networks
Authors: Krasnov Boris R. , Khokhlova Irina S. , Berrizbeitia M. Fernanda López , Matthee Sonja , Sanchez Juliana P. , van der Mescht Luther 
Published: May 2024

AbstractFunctional signal in an interaction network is a phenomenon in which species resembling each other in their traits interact with similar partners. We tested the functional signal concept in realm-specific and regional flea-host networks from four biogeographic realms and asked whether the species composition of (a) host spectra and (b) flea assemblages is similar between functionally similar flea and host species, respectively. Analogously to testing for phylogenetic signal, we applied Mantel tests to investigate the correlation between flea or host functional distances calculated from functional dendrograms and dissimilarities in sets of interacting partners. In all realm-specific networks, functionally similar fleas tended to exploit similar hosts often belonging to the same genus, whereas functionally similar hosts tended to harbour similar fleas, again often belonging to the same genus. The strength of realm-specific functional signals and the frequency of detecting a significant functional signal in the regional networks differed between realms. The frequency of detecting a significant functional signal in the regional networks correlated positively with the network size for fleas and with the number of hosts in a network for hosts. A functional signal in the regional networks was more frequently found for hosts than for fleas. We discuss the mechanisms behind the functional signal in both fleas and their hosts, relate geographic functional signal patterns to the historic biogeography of fleas and conclude that functional signals in the species composition of host spectra for fleas and of flea assemblages for hosts result from the interplay of evolutionary and ecological processes.