bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid diagnosis of rinderpest by Countercurrent Immuno‐electro‐osmo‐phoresis in Nigeria
Authors: Durojaiye O. A. 
Published: August 2010

SummaryNineteen samples of tissues obtained at autopsy in recent suspected outbreaks of rinderpest in Nigeria were tested by the Ouchterlony gel diffusion and countercurrent immuno‐electro‐osmo‐phoresis (CIEOP) techniques. Rinderpest virus antigen was detected by CIEOP in 11 samples (within 30 minutes) and by the Ouchterlony technique in 8 of the samples.ZusammenfassungSchnell‐Diagnose der Rinderpest durch gegenläufige Immun‐Elektroosmophorese in NigeriaNeunzehn bei der Autopsie gewonnene Gewebeproben nach einigen fraglichen, in letzter Zeit aufgetretenen Ausbrüchen von Rinderpest in Nigeria wurden mit der Ouchter‐lony‐Geldiffusions‐Technik und der gegenläufigen Immun‐Elektroosmophorese (CIEOP) untersucht. Rinderpestvirus‐Antigen konnte mit der CIEOP in elf Proben nachgewiesen werden (und zwar innerhalb 30 Minuten), mit der Ouchterlony‐Technik in acht der Proben.ResumenEl diagnóstico rápido de la peste bovina mediante inmunoelectroosmoforesis de contracorriente en NigeriaDiecinueve muestras de tejidos obtenidas durante las autopsias tras algunos brotes sospechosos recientes de peste bovina en Nigeria fueron examinadas con la técnica de Ouchterlony de difusión en gelosa y la de inmunoelectroosmoforesis de contracorriente (CIEOP). Antígeno de virus peste bovina se pudo poner en evidencia con la CIEOP en once muestras (en el espacio de 30 minutos), mientras que con la técnica de Ouchterlony en ocho muestras.
Isolation of Acinetobacter calcoaceticus from a Cow with Mastitis
Authors: Rahman H. , Baxi K. K. 
Published: August 2010

SummaryA case of clinical mastitis in a cow with Acinetobacter calcoaceticus was recorded. The isolate was sensitive to neomycin, chloramphenicol, erythromycin and streptomycin, and of resistant to penicillin, tetracycline and nitrofurantoin.
Vergleichende Untersuchungen zur Anwendung eines Enzyme‐linked‐Immunosorbent‐Assay (ELISA) zum Antikörpernachweis gegen den Erreger der Aviären Encephalomyelitis*
Authors: Richter R. , Kösters J. , Kuhavanta‐Kalkosol S. 
Published: August 2010

ZusammenfassungAuf Grundlage eines teilgereinigten und konzentrierten AEV‐Antigens aus infiziertem embryonalen Gehirn wurde ein ELISA entwickelt. In vergleichenden Untersuchungen mit AGP, VN und EET wurde festgestellt, daß der ELISA eine genügend hohe Sensitivität besitzt, um quantitative Aussagen machen zu können. Die Methode ist empfindlicher als der AGP. Der ELISA ist für die routinemäßige Untersuchung von Hühnerseren auf Antikörper gegen AEV geeignet. Die Lagerfähigkeit beschichteter vorbereiteter ELISA‐Platten ist auf 4 Wochen bei + 4°C bzw. 5 Wochen bei −20 °C beschränkt.SummaryComparative studies on the use of an Enzyme‐linked Irnmunosorbent Assay (ELISA) for the Dethction of Antibodies against the Avian Encephalomyelitis‐Virus (AEV)The present work was concerned with assessing an ELISA on the basis of a partially purified and concentrated AEV‐antigen from infected embryonic brain tissue.In comparison with the agar‐gel‐precipitin‐test (AGP), virus neutralization test and embryo susceptibility test it was shown that the ELISA is sensitive enough to ensure a quantitative result. The method is more sensitive than AGP. The ELISA is suitable for the routine diagnosis of AEV‐antibodies in chicken sera.The storage of antigen‐covered prepared ELISA plates is possible for 4 weeks at 4 °C and for 5 weeks at −20 °C.RésuméRecherches comparées sur l'utilisation d'un Enzyme‐linked‐immunosorbent‐Assay (ELISA) pour la mise en évidence d'anticorps contre l'agent de l'encéphalomyélite aviaire Un test ELISA a été développé sur la base d'un antigéne AEV partiellement purifié et concentré à partir d'un cerveau embryonnaire infecté. Dans des recherches comparées avec AGP, VN et EET, il a été établi que l'ELISA posséde une sensibilité suffisamment élevée pour pouvoir donner des résultats quantitatifs. La méthode est plus sensible que AGP. ELISA peut être utilisé dans les examens de routine pour la recherche des anticorps contre AEV dans des sérums de volaille. La durée de conservation des plaques ELISA préparées est limitée à 4 semaines à + 4°C et à 5 semaines à — 20°C.ResumenEstudios comparativos sobre la aplicación de un ensayo inmunosorbente ligado a la enzima (ELISA) para la puesta en evidencia de anticuerpos frente al agente etiológico de la encefalomielitis aviarSe desarrolló un ELISA sobre la base de un antígeno semipurificado y concentrado de AEV procedente de cerebro embrionario infectado. En investigaciones comparadas con AGP, VN y EET se comprobó que el ELISA posee una sensitividad elevada suficiente para poder hacer aseveraciones cuantitativas. Este método es más sensible que el de la AGP. El ELISA resulta apropiado para el análisis rutinario de sueros sanguíneos de gallina en cuanto a anticuerpos frente a AEV. Se halla limitado a 4 semanas a ± 4 °C o a 5 semanas a −20 °C, resp. El tiempo de almacenamiento de placas ELISA preparadas por estratos.
The existence of three different viral agents in a tumour bearing European eel (Anguilla anguilla)
Authors: Ahne W. , Thomsen I. 
Published: August 2010

SummaryThree different viral agents were isolated from a tumour bearing eel. Two of the agents were identified as IPNV‐subtype Ab and as Rhabdovirus anguilla subtype EVX. The third syncytia‐forming agent has not as yet been classified.ZusammenfassungDas Vorkommen 3 verschiedener Viren bei einem Aal mit StomatopapillomVon einem tumortragenden Aal konnten 3 verschiedene Viren isoliert werden. 2 der Erreger wurden als IPNV‐Subtyp Ab und als Rhabdovirus anguilla‐Subtyp EVX identifiziert. Das 3.syncytienbildende Agens konnte bisher noch nicht klassifiziert werden.RésuméPrésence de trois virus différents dans un stomatopapillome chez une anguilleTrois virus différents ont pu être isolés chez une anguille atteinte d'une tumeur. Deux virus ont été identifiés comme IPNV — sous‐type Ab et comme Rhabdovirus anguilla, sous‐type EVX. Le troisième agent, syncytia‐formateur, n'a pas pu être encore classé.ResumenLa existencia de tres agentes virales diferentes en una anguila europea (Anguilla anguilla) con estomatopapilomaDe una anguila, que era portadora de un tumor, se pudieron aislar 3 virus diferentes. 2 de estos agentes fueron identificados como IPNV‐subtipo Ab y como Rhabdovirus anguilla — subtipo EVX. El tercer agente, sinciciógeno, no pudo ser clasificado hasta la fecha.
The Effect of Sex Hormones on Total Immunoglobulin Level in the Serum of Moulting Hens
Authors: Bobek S. , Mika M. , Niezgoda J. , Pierzchala K. 
Published: August 2010

SummaryOestradiol given separately increased the concentration of immunoglobulin in the blood plasma of moulting hens. Progesterone decreased the level of Ig, while testosterone had no effect, but both hormones potentiated the effect of oestradiol.ZusammenfassungDer Einfluß von Geschlechtshormonen auf die Serum‐Immunglobulinspiegel in Hühnern während der MauserDie gesonderte Gabe von Oestradiol erhöhte die Immunglobulinkonzentration im Plama von Hühnern während der Mauser. Progesteron wirkte depressiv auf die Ig‐Spiegel, Testosteron war ohne Effekt. Bei gleichzeitiger Verabreichung potenzierten beide jedoch den Effekt von Oestradiol.
Inhibición del crecimiento de poblaciones bacterianas mediante beta glucósidos extraídos de Myzodendron punctulatum B y S3
Authors: García‐Quintana H. G. , Barrera D. , Reyes A. , Polette M. 
Published: August 2010

ResumenSe analiza el efecto antibacteriano de tres nuevos beta glucósidos extraídos de M. punctulatum B y S sobre 24 clones bacterianos pertenecientes a seis especies diferentes. El product 04(4‐hidroxi‐3‐O‐glucosil fenil)‐z‐butanona es el que posee mayor efecto inhibito‐rio. Por su parte St. aureus resulta la especie más sensible. Se continúan los estudios tendientes a incrementar la actividad inhibitoria de estas sustancias.ZusammenfassungDie hemmende Wirkung sekundärer Metaboliten von Myzodendron punctulatum B und S auf das Wachstum verschiedener BakterienstämmeEs wurde die letale oder hemmende Wirkung von drei neuen sekundären Metaboliten, isoliert von Myzodendron punctulatum B und S, an 24 Bakterienstämmen aus 6 verschiedenen Gruppen untersucht. Es zeigte sich, daß der Metabolit 4‐(4‐hydroxi‐3‐0‐glucosyl phenyl)‐2‐butanone die größte hemmende Wirkung aufwies. Dieser Effekt wurde an 8 Stämmen beobachtet. Bei den Bakterienstämmen reagierte Staphylococcus aureus am empfindlichsten. Es werden weitere Untersuchungen durchgeführt werden, um die hemmende Wirkung dieser Substanzen zu erhöhen.SummaryInhibitory effect on bacterial growth by beta glucosids obtained from Myzodendron punctulatum B and SThe lethal or inhibitory effect of three new secondary metabolites obtained from Myzodendron punctulatum B and S was studied on 24 bacterial strains of six different species. It was concluded that the substance with more inhibitory activity was the metabolite 4‐(4‐hydroxi‐3‐O‐glucosyl phenyl)‐2‐butanone as it was active on eight strains. On the other hand Staphylococcus aureus was found to be the most sensitive species. Further studies are being carried out to increase the inhibitory activity of these substances.RésuméEffet inhibiteur des métabolites secondaires de Myzodendron punctulatum B et S sur la croissance de différentes souches bactériennesL'effet létal ou inhibiteur de trois nouveaux métabolites secondaires isolés de Myzodendron punctulatum B et S a été examiné sur 24 souches de bactiries de 6 groupes différents. Le metabolite 4‐(4‐hydroxi‐3‐0‐glucosyl phenyl)‐2‐butanone a présenté le plus grand effet inhibiteur. Cet effet a été observé sur 8 souches. Staphylococcus aureus a été le plus sensible parmi les souches bactériennes. D'autres recherches ont été entreprises pour augmenter I'effet inhibiteur de ces substances.
Association of DRD4 polymorphism with severity of oppositional defiant disorder, separation anxiety disorder and repetitive behaviors in children with autism spectrum disorder
Authors: Gadow Kenneth D. , DeVincent Carla J. , Olvet Doreen M. , Pisarevskaya Victoria , Hatchwell Eli 
Published: August 2010

AbstractThe objective was to examine whether a common polymorphism in the dopamine D4 receptor gene (DRD4) might be a potential biomarker for behavioral variation within the autism spectrum disorder clinical phenotype. Children (N = 66) were evaluated with a validated mother‐ and teacher‐completed DSM‐IV‐referenced rating scale. Partial eta‐squared (ηp2) was used to gauge the magnitude of group differences: 0.01−0.06 = small, 0.06−0.14 = moderate and > 0.14 = large. Children who were 7‐repeat allele carriers had more severe oppositional defiant disorder behaviors according to mothers’ (ηp2 = 0.10) and teachers’ (ηp2 = 0.06) ratings than noncarriers, but the latter was marginally significant (P = 0.07). Children who were 7‐repeat allele carriers also obtained more severe maternal ratings of tics (ηp2 = 0.07) and obsessions–compulsions (ηp2 = 0.08). Findings for maternal ratings of separation anxiety were marginally significant (P = 0.08, ηp2 = 0.05). Analyses of combined DRD4 and dopamine transporter gene (DAT1) genotypes approached significance (P = 0.05) for teachers’ ratings of oppositional behavior and mothers’ ratings of tics. DRD4 allelic variation may be a prognostic biomarker for challenging behaviors in children with autism spectrum disorder, but these exploratory findings remain tentative pending replication with larger independent samples.
Immunohistochemical analysis of the progression of flat and papillary preneoplastic lesions in intrahepatic cholangiocarcinogenesis in hepatolithiasis
Authors: Itatsu Keita , Zen Yoh , Ohira Shusaku , Ishikawa Akira , Sato Yasunori , Harada Kenichi , Ikeda Hiroko , Sasaki Motoko , Nimura Yuji , Nakanuma Yasuni 
Published: August 2010

AbstractBackground: Two types of precursor lesions, flat‐type ‘biliary intraepithelial neoplasia (BilIN)’ and papillary‐type ‘intraductal papillary neoplasm of the bile duct (IPNB)’, are proposed in the tumorigenesis of intrahepatic cholangiocarcinoma (ICC) in hepatolithiasis.Methods: In this study, the participation of cancer‐related molecules in the progression of these two precursor lesions was examined, using 64 hepatolithiatic livers with BilIN lesions (45 livers) and IPNB lesions (19 livers) and 10 hepatolithiatic livers without neoplastic lesions as a control. The expression of E‐cadherin, β‐catenin, matrix metalloproteinase‐7 (MMP‐7), membrane type 1‐MMP (MT1‐MMP), cyclin D1 and c‐myc was immunohistochemically examined.Results: The membranous expression of β‐catenin decreased along with the progression in both BilIN and IPNB lineages. Membranous expression of E‐cadherin was significantly decreased in invasive ICC with BilIN and IPNB in comparison with non‐invasive counterparts. MMP‐7 and MT1‐MMP were commonly expressed in invasive ICC with BilIN (100%), while non‐invasive lesions (BilIN‐1, ‐2, ‐3) and the IPNB lineage were only occasionally and weakly positive for these molecules. Cyclin D1 and c‐myc, target molecules of Wnt signalling, were frequently positive in the IPNB lineage (65 and 54% respectively), and interestingly nuclear β‐catenin staining, reflecting activation of Wnt signalling, was observed only in the IPNB lineage (22%) (P<0.05).Conclusions: Decreased membranous expression of β‐catenin and E‐cadherin is an early event in the tumorigenesis of both BilIN and IPNB lineages. The expression of MMP‐7 and MT1‐MMP was closely associated with invasive growth of the BilIN lineage. The Wnt signalling pathway may play an important role in the tumorigenesis of the IPNB lineage.
Intra‐operative ultrasound for detection of liver metastases from colorectal cancer
Authors: Mazzoni Gianluca , Napoli Alessandro , Mandetta Saverio , Miccini Michelangelo , Cassini Diletta , Gregori Matteo , Colace Lidia , Tocchi Adriano 
Published: August 2010

AbstractObjective: The aim of this study was to evaluate the accuracy of intra‐operative ultrasound (IOUS) imaging in detecting liver secondaries at the time of primary colorectal surgery and to evaluate the impact of IOUS on patient management.Methods: Data from 167 patients with primary colorectal cancer who were admitted for elective surgery between January 1995 and December 2003 were prospectively evaluated and analysed. All patients underwent pre‐operative abdominal ultrasonography (US) and computed tomography (CT), as well as IOUS. The final diagnosis of liver metastases was made by means of histological examination of either biopsy or surgical specimens. The sensitivities of pre‐operative US and CT were compared with the sensitivity of IOUS, referred to histology. Changes in surgical management owing to IOUS findings were noted.Results: IOUS supplied additional information in the case of 31 patients. In 28 of these patients, this information had a major impact on the intra‐operative strategy, in that the procedure was altered.Conclusions: IOUS is safe, simple to perform and more accurate than pre‐operative imaging. It reduces the number of patients subjected to superfluous surgery. The use of IOUS is therefore encouraged during colorectal cancer surgery.
Non‐transplant surgical approach to liver‐based hereditary haemorrhagic telangiectasia: a first report
Authors: Orlando Giuseppe , Sabbà Carlo , Carino Nicola De Liguori , Scelzo Chiara , Tariciotti Laura , Bonanni Luigi , Angelo Maurizio D' , Baiocchi Leonardo , Lerut Jan , Tisone Giuseppe 
Published: August 2010

AbstractA 55‐year‐old woman with hereditary haemorrhagic telangiectasia (HHT) underwent a left lateral liver bisegmentectomy (removal of segments 2 and 3) for hepatic‐based arteriovenous malformations. This lesion determined a progressive fatigue and invalidating effort dyspnoea. The postoperative course was uneventful and the patient is currently doing well at 4 years after surgery. To our knowledge, this is the first case of hepatic‐based HHT treated with liver resection. This anecdotal report should promote the evaluation of this approach in order to define its role in the treatment of liver involvement in this rare disease.
Methylprednisolone injection via the portal vein suppresses inflammation in acute liver failure induced in rats by lipopolysaccharide and d‐galactosamine
Authors: Higuchi Nobito , Kato Masaki , Kotoh Kazuhiro , Kohjima Motoyuki , Aishima Shinichi , Nakamuta Makoto , Fukui Yoshinori , Takayanagi Ryoichi , Enjoji Munechika 
Published: August 2010

AbstractBackground: We have reported that hepatic arterial steroid injection is an effective therapy to rescue patients from fulminant or severe acute hepatic failure. We speculate that a high concentration of steroid suppresses inflammatory processes in the liver directly by restraining activated inflammatory cells, including macrophages. To analyse the detailed mechanism, steroid injection via the portal vein was performed in an experimental model of liver damage.Methods: Rats subjected to lipopolysaccharide and d‐galactosamine injection were treated with a methylprednisolone injection via the tail vein or the portal vein. The survival rate, serum levels of inflammatory cytokines and apoptotic cell counts in the liver were analysed.Results: The survival rate was significantly improved by steroid injection, especially via the portal vein. Serum values of alanine aminotransferase, tumor necrosis factor‐α and interferon‐γ were reduced in the treated groups, especially the group given portal venous injections. Apoptotic cell counts in the liver were significantly lower in the group injected with steroid via the portal vein.Conclusion: In the model rats, high concentrations of steroid in the liver acted on inflammatory cells and suppressed inflammatory cytokines and liver cell death. The mechanism is suggested to be the same for arterial steroid injection therapy in patients with acute hepatic failure.
Acute hepatitis in a patient with familial Mediterranean fever
Authors: Migita Kiyoshi , Abiru Seigo , Tanaka Mototsugu , Ito Masahiro , Miyashita Taichiro , Maeda Yumi , Koga Tomohiro , Nakamura Minoru , Komori Atsumasa , Yatsuhashi Hiroshi , Ida Hiroaki , Eguchi Katsumi , Hirayama Kenji , Yasunami Michio , Ishibashi Hiromi 
Published: August 2010

AbstractFamilial Mediterranean fever (FMF) is a hereditary syndrome characterized by recurrent episodes of fever and serositis. In this report, we describe a Japanese patient with FMF and Sjögren's syndrome, in whom acute elevations of transaminase occurred. The histological findings from the liver biopsy specimens demonstrated a nonspecific hepatitis, with liver cell necrosis and interlobular inflammatory cell invasion, without the presence of interface hepatitis or bile duct injury. This case underscores the possibility that MEFV mutations contribute to hepatic inflammation, as seen in this case, by way of an alteration of the pyrin function.
Connective tissue growth factor, steatosis and fibrosis in patients with chronic hepatitis C
Authors: Hora Caroline , Negro Francesco , Leandro Giacchino , Oneta Carl M. , Rubbia‐Brandt Laura , Muellhaupt Beat , Helbling Beat , Malinverni Raffaele , Gonvers Jean‐Jacques , Dufour Jean‐François ,   the Swiss Hepatitis C Cohort Study Group
Published: August 2010

AbstractBackground/Aim: Both steatosis and insulin resistance have been linked to accelerated fibrosis in chronic hepatitis C. Connective tissue growth factor (CTGF) plays a major role in extracellular matrix production in fibrotic disorders including cirrhosis, and its expression is stimulated in vitro by insulin and glucose. We hypothesized that CTGF may link steatosis, insulin resistance and fibrosis.Methods: We included 153 chronic hepatitis C patients enrolled in the Swiss Hepatitis C Cohort Study and for whom a liver biopsy and plasma samples were available. CTGF expression was assessed quantitatively by immunohistochemistry. In 94 patients (57 with genotypes non‐3), plasma levels of glucose, insulin and leptin were also measured. CTGF synthesis was investigated by immunoblotting on LX‐2 stellate cells.Results: Connective tissue growth factor expression was higher in patients with steatosis (P=0.039) and in patients with fibrosis (P=0.008) than those without these features. CTGF levels were neither associated with insulinaemia or with glycaemia, nor with inflammation. By multiple regression analysis, CTGF levels were independently associated with steatosis, a past history of alcohol abuse, plasma leptin and HCV RNA levels; when only patients with genotypes non‐3 were considered, CTGF levels were independently associated with a past history of alcohol abuse, plasma leptin levels and steatosis. Leptin stimulated CTGF synthesis in LX‐2 cells.Conclusions: In patients with chronic hepatitis C and steatosis, CTGF may promote fibrosis independently of inflammation. CTGF may link steatosis and fibrosis via increased leptin levels.
Changes in serum hepatitis B e antigen (HBeAg) levels associated with the emergence of YMDD mutants in HBeAg non‐seroconverted patients during lamivudine therapy
Authors: Yen Yi‐Hao , Lu Sheng‐Nan , Chen Chien‐Hung , Wang Jing‐Houng , Wu Chun‐Mei , Hung Chao‐Hung , Tseng Po‐lin , Hu Tsung‐Hui , Changchien Chi‐Sin , Lee Chuan‐Mo 
Published: August 2010

AbstractBackground: To elucidate the associations between the changing patterns of hepatitis B e antigen (HBeAg) levels and the emergence of tyrosine–methionine–aspartate–aspartate (YMDD) mutants in HBeAg non‐seroconverted patients undergoing lamivudine therapy.Methods: This study analysed 76 HBeAg‐positive naïve chronic hepatitis B patients treated with lamivudine. The median duration of therapy was 52 weeks. The YMDD mutants were detected in 35 patients. The changing patterns of HBeAg levels were categorized into three groups: Descending, Descending–Ascending and Fluctuation. HBeAg breakthrough was defined as progressive HBeAg decreasing to <10% of pretreatment levels, followed by increases exceeding 50 S/Co [the ratio of the sample (S) to the cut‐off (Co)] above nadir levels.Results: Of 76 patients, the sensitivity and specificity for predicting YMDD mutants by the Descending–Ascending pattern were 66 and 100% respectively. Of 17 patients with YMDD mutants in the Descending–Ascending group, hepatitis B virus (HBV) DNA first increased, followed by increased HBeAg levels and finally by biochemical breakthrough. The median intervals between virological breakthrough and HBeAg breakthrough, between HBeAg breakthrough and biochemical breakthrough and between virological breakthrough and biochemical breakthrough were 4, 24 and 33 weeks respectively.Conclusions: Serial HBeAg levels are useful in predicting YMDD mutant emergence in HBeAg non‐seroconverted patients during lamivudine therapy.