bluuebunny/crossref_metadata_2025_split huggingface dataset:
Zur Frage der Erfassung und Charakterisierung der Dissoziationsformen von Brucella abortus*
Authors: Meyn A. , Schmid D. O. , Schrinner E. J. 
Published: September 2010

ZusammenfassungDer 1931 in der Brucellen‐Diagnostik von Alessandrini‐Sabatucci (1) und Pampana (144) eingeführte Trypaflavin‐Test stellt nach zahlreichen eigenen vergleichenden Untersuchungen die einzige Möglichkeit dar, rasch und zuverlässig reine S‐Formen von “Pseudo‐S‐Formen mit R‐Charakter” sowie von anderen übergangs‐ und Mischformen, deren Einordnung in das weite Brucella‐Phasenspektrum nicht immer möglich ist, abzugrenzen. Im Gegensatz zu allen übrigen Formen tritt bei reinen S‐Formen in einer Trypaflavin‐Lösung 1 : 1000 keine Spontanagglutination ein.SummaryContribution to the understanding and characterisation of the dissociation forms of Brucella abortusNumerous comparative studies carried out by the author have shown that trypaflavine test introduced by Alessandrini‐Sabatucci (1) and Pampana (144) in 1931 for the bacteriological diagnosis of Brucella abortus is the only one capable of differentiating rapidly and surely the pure S‐forms from the ‘pseudo‐S‐forms with R‐character’, as well as their intermediate and mixed forms, the classification of which in the broad Brucella phase spectrum is not always possible. Contrary to all other forms, the pure S‐forms in a trypaflavine solution (1 : 1000) do not give spontaneous agglutination.RésuméComment déceler et caractériser les formes de dissociation de Brucella abortusLe test à la trypaflavine, introduit en 1931 dans le diagnostic bactériologique des Brucella par Alessandrini‐Sabatucci et Pampana (1944) représente, comme l'ont montré les nombreuses recherches comparées entreprises par l'auteur, la seule possibilité de différencier rapidement et avec certitude les formes S‐pures des “pseudo‐formes S avec caractère R” ainsi que d'autres formes intermédiaires ou mélangées dont la classification dans le spectre étendu des Brucella n'est pas toujours possible. Contrairement toutes les autres formes, les formes S pures ne donnent aucune agglutination spontanée dans une solution de trypaflavine au 1/1000e.ResumenSobre la cuestión del englobamiento y caracterización de las formas de disociación de la Brucella abortusCon arreglo a múltiples estudios propios comparativos, la prueba de la tripaflavina, intriducida en 1931 en el diagnóstico brucelar por Alessandrini‐Sabatucci (1) y Pampana (1944), representa la única posibilidad de delimitar rápidamente y con seguridad las formas S puras de la “formas pseudo‐S con carácter R” y otras formas de transición y mixtas, cuya inclusión en el amplio espectro brucelar de fases no siempre resulta posible. En contraste con todas las formas restantes, en las formas lisas puras en solución de tripaflavina al 1 por mil no se produce la aglutinación espontánea.
Ein Teratom der Chiasmagegend beim Kaninchen
Authors: Kersting Günter , Hammersen Frithjof 
Published: September 2010

ZusammenfassungEin bei einem Kaninchen beobachteter Mißbildungstumor mit geweblichen Anteilen aller drei Keimblätter im Bereich des mediobasalen Stirnhirns ist Gegenstand der Darstellung. Neben der großen Seltenheit derartiger Teratome im Tierreich gewinnt der beschriebene Fall eine besondere Bedeutung durch die im Anschluß an eine Infektion mit dem Virus der westlichen Pferdeencephalitis aufgetretenen onkolytischen Alterationen.SummaryA teratoma in the region of the optic chiasma in the rabbitThis is a report of a teratoma with tissues derived from all three germ layers and situated in the medio‐basal region of the frontal lobe. Apart from the extreme rarity of such tumours in animals, the case assumes particular importance because the tumour showed areas of recent focal necrosis which may have been due to inoculation with the virus of Western equine encephalomyelitis. (The animal was being used to produce hyper‐immune serum.)RésuméUn dysembryome de la région du chiasma optique chez le lapinUne tumeur embryonale comprenant des tissus dérivés des trois feuillets a été trouvée dans la région médio‐basale du lobe frontal et est l'objet d'une description. Outre l'extrême rareté de tels dysembryomes dans le règne animal, le cas rapporté dans cet article prend une importance particulière du fait que cette tumeur présentait des alterations oncolytiques survenues après une infection par le virus ouest de l'encéphalite du cheval.ResumenUn teratome en la región quiásmica del conejoEl objeto de la descripción es un tumor deformante con partes hísticas pertenecientes a las tres hojas blastodérmicas en la zona del lóbulo frontal mediobasal de un conejo. Al lado de la enorme rareza de tales teratomas en el reino animal, el caso descrito tiene una importancia significativa por las alteraciones oncolíticas aparecidas a continuación de la infección con el virus de la encefalomielitis equina tipo occidental.
Hereditary angioedema: an update on available therapeutic options
Authors: Maurer Marcus , Magerl Markus 
Published: September 2010

SummaryThere is no cure for hereditary angioedema (HAE). Therapeutic approaches consist of symptomatic therapy for acute attacks, short‐term prophylaxis before surgery, and long‐term prophylaxis for those with frequent and severe attacks.In Germany, C1‐INH concentrate and icatibant are licensed for acute therapy. C1‐INH concentrate, which is obtained from human plasma, is administered intravenously to restore the deficient C1‐INH activity. This therapy, which has been available for decades, is effective and well‐tolerated. Batch documentation is required by German law. The synthetic decapeptide icatibant is administered subcutaneously. It competes with bradykinin, the responsible inducer of edema formation, for binding to the bradykinin B2 receptor. Icatibant is also effective and well‐tolerated, even on repeated administration. An additional human C1‐inhibitor, a recombinant human C1‐inhibitor and the recombinant inhibitor of kallikrein ecallantide are currently under development.There are no licensed treatment options available in Germany for long‐ and short‐term prophylaxis. Androgen derivatives are established in long‐term prophylaxis. However, they are associated with many adverse effects, some of which are severe. Many drug interactions also limit their use. They are contraindicated in pregnancy, lactation, for children and in cases of prostate cancer. Antifibrinolytics have fewer adverse effects but are also less effective than androgens. They are contraindicated in thromboembolic disease and impaired vision. If androgen therapy has too negative an effect on quality of life, it may be worth reducing the dose or discontinuing therapy entirely and treating attacks with acute therapy.
Identification of Potential Diagnostic Markers among
Burkholderia cenocepacia
and
B. multivorans
Supernatants
Authors: Mott Tiffany , Soler Michael , Grigsby Sierrah , Medley Ronald , Whitlock Gregory C. 
Published: September 2010

ABSTRACT

Patients with cystic fibrosis (CF) are susceptible to chronic respiratory infections with a number of bacterial pathogens. Among them, the
Burkholderia cepacia
complex (Bcc) bacteria, consisting of nine related species, have emerged as problematic CF pathogens due to their antibiotic resistance, incidence of nosocomial infection, and person-to-person transmission. Bcc organisms present the clinical microbiologist with a diagnostic dilemma due to the lack of phenotypic biochemical or growth-related characterization tests that reliably distinguish among these organisms. The complex taxonomy of the Bcc species colonizing the CF respiratory tract makes accurate identification problematic. Despite the clinical implications of Bcc identification, a clinical laboratory differentiation of species within the Bcc is lacking. Additionally, no commercial assays are available to further identify the Bcc species. In the current study, secretory proteins present in the cultured supernatants of
Burkholderia cenocepacia
and
Burkholderia multivorans
were analyzed by two-dimensional gel electrophoresis (2-DE), followed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). To assess differential expression, protein spots of
B. cenocepacia
and
B. multivorans
that were unique or displayed different intensities were chosen for MALDI-TOF MS analysis. In total, 341 protein spots were detected, of which 23 were unique to each species, demonstrating that potential diagnostic candidates between these two members of the Bcc exist.
Efficient Differentiation ofMycobacterium aviumComplex Species and Subspecies by Use of Five-Target Multiplex PCR
Authors: Shin Sung Jae , Lee Byung Soo , Koh Won-Jung , Manning Elizabeth J. B. , Anklam Kelly , Sreevatsan Srinand , Lambrecht Randall S. , Collins Michael T. 
Published: September 2010

ABSTRACTInfections caused by theMycobacterium aviumcomplex (MAC) are on the rise in both human and veterinary medicine. A means of effectively discriminating among closely related yet pathogenetically diverse members of the MAC would enable better diagnosis and treatment as well as further our understanding of the epidemiology of these pathogens. In this study, a five-target multiplex PCR designed to discriminate MAC organisms isolated from liquid culture media was developed. This MAC multiplex was designed to amplify a 16S rRNA gene target common to allMycobacteriumspecies, a chromosomal target called DT1 that is unique toM. aviumsubsp.aviumserotypes 2 and 3, toM. aviumsubsp.silvaticum, and toM. intracellulare, and three insertion sequences, IS900, IS901, and IS1311.The pattern of amplification results allowed determination of whether isolates were mycobacteria, whether they were members of the MAC, and whether they belonged to one of three major MAC subspecies,M. aviumsubsp.paratuberculosis,M. aviumsubsp.avium, andM. aviumsubsp.hominissuis. Analytical sensitivity was 10 fg ofM. aviumsubsp.paratuberculosisgenomic DNA, 5 to 10 fg ofM. aviumsubsp.aviumgenomic DNA, and 2 to 5 fg of DNA from other mycobacterial species. Identification accuracy of the MAC multiplex was evaluated by testing 53 bacterial reference strains consisting of 28 different mycobacterial species and 12 nonmycobacterial species. Identification accuracy in a clinical setting was evaluated for 223 clinical MAC isolates independently identified by other methods. Isolate identification agreement between the MAC multiplex and these comparison assays was 100%. The novel MAC multiplex is a rapid, reliable, and simple assay for discrimination of MAC species and subspecies in liquid culture media.
Chronic Shedders as Reservoir for Nosocomial Transmission of Norovirus
Authors: Sukhrie Faizel H. A. , Siebenga J. Joukje , Beersma Matthias F. C. , Koopmans Marion 
Published: September 2010

ABSTRACT
Norovirus (NoV) infection in immunocompromised patients may lead to prolonged norovirus shedding. Here, we demonstrate the involvement of three chronic shedders in hospital outbreaks. Combined epidemiological and molecular evidence suggests that in one case, NoV transmission occurred at least 17 days after the first diagnosis.
Immunization with Plant-Expressed Hemagglutinin Protects Chickens from Lethal Highly Pathogenic Avian Influenza Virus H5N1 Challenge Infection
Authors: Kalthoff Donata , Giritch Anatoli , Geisler Katharina , Bettmann Ulrike , Klimyuk Victor , Hehnen Hans-Robert , Gleba Yuri , Beer Martin 
Published: September 2010

ABSTRACT
Highly pathogenic avian influenza (HPAI) is a striking disease in susceptible poultry, which leads to severe economic losses. Inactivated vaccines are the most widely used vaccines in avian influenza virus (AIV) vaccination programs. However, these vaccines interfere with the serological detection of wild-type AIV infections in immunized populations. The use of vaccines that allow differentiation between infected and vaccinated animals (DIVA strategy) would stop current stamping-out policies. Therefore, novel vaccination strategies are needed to allow improved protection of animals and humans against HPAI virus (HPAIV) infection. The presented study analyzed for the first time the immunogenic capacity of plant-expressed full-length hemagglutinin (rHA0) of HPAIV H5N1 in several vaccine formulations within the highly relevant host species chicken. We were able to express plant-expressed rHA0 at high levels and could show that, when administered with potent adjuvants, it is highly immunogenic and can fully protect chicken against lethal challenge infection. Real-time reverse transcription (RT)-PCR and serological tests demonstrated only marginally increased virus replication in animals vaccinated with plant-derived rHA0 compared to animals immunized with an inactivated reference vaccine. In addition, the use of plant-expressed rHA0 also allowed an easy serological differentiation of vaccinated from AIV-infected animals based on antibodies against the influenza virus NP protein.
A Live Attenuated H7N7 Candidate Vaccine Virus Induces Neutralizing Antibody That Confers Protection from Challenge in Mice, Ferrets, and Monkeys
Authors: Min Ji-Young , Vogel Leatrice , Matsuoka Yumiko , Lu Bin , Swayne David , Jin Hong , Kemble George , Subbarao Kanta 
Published: September 2010

ABSTRACTA live attenuated H7N7 candidate vaccine virus was generated by reverse genetics using the modified hemagglutinin (HA) and neuraminidase (NA) genes of highly pathogenic (HP) A/Netherlands/219/03 (NL/03) (H7N7) wild-type (wt) virus and the six internal protein genes of the cold-adapted (ca) A/Ann Arbor/6/60ca(AAca) (H2N2) virus. The reassortant H7N7 NL/03cavaccine virus was temperature sensitive and attenuated in mice, ferrets, and African green monkeys (AGMs). Intranasal (i.n.) administration of a single dose of the H7N7 NL/03cavaccine virus fully protected mice from lethal challenge with homologous and heterologous H7 viruses from Eurasian and North American lineages. Two doses of the H7N7 NL/03cavaccine induced neutralizing antibodies in serum and provided complete protection from pulmonary replication of homologous and heterologous wild-type H7 challenge viruses in mice and ferrets. One dose of the H7N7 NL/03cavaccine elicited an antibody response in one of three AGMs that was completely protected from pulmonary replication of the homologous wild-type H7 challenge virus. The contribution of CD8+and/or CD4+T cells to the vaccine-induced protection of mice was evaluated by T-cell depletion; T lymphocytes were not essential for the vaccine-induced protection from lethal challenge with H7wtviruses. Additionally, passively transferred neutralizing antibody induced by the H7N7 NL/03cavirus protected mice from lethality following challenge with H7wtviruses. The safety, immunogenicity, and efficacy of the H7N7 NL/03cavaccine virus in mice, ferrets, and AGMs support the evaluation of this vaccine virus in phase I clinical trials.
AN ALGEBRAIC CHARACTERIZATION OF SIMPLE CLOSED CURVES ON SURFACES WITH BOUNDARY
Authors: CHAS MOIRA , KRONGOLD FABIANA 
Published: September 2010

We prove that a conjugacy class in the fundamental group of a surface with boundary is represented by a power of a simple curve if and only if the Goldman bracket of two different powers of this class, one of them larger than two, is zero. The main theorem actually counts self-intersection number of a primitive class by counting the number of terms of the Goldman bracket of two distinct powers, one of them larger than two.
A New General Iterative Method for a Finite Family of Nonexpansive Mappings in Hilbert Spaces
Authors: Singthong Urailuk , Suantai Suthep 
Published: September 2010

AbstractWe introduce a new general iterative method by using the "Equation missing"-mapping for finding a common fixed point of a finite family of nonexpansive mappings in the framework of Hilbert spaces. A strong convergence theorem of the purposed iterative method is established under some certain control conditions. Our results improve and extend the results announced by many others.
Pan-histone deacetylase inhibitor panobinostat depletes CXCR4 levels and signaling and exerts synergistic antimyeloid activity in combination with CXCR4 antagonists
Authors: Mandawat Aditya , Fiskus Warren , Buckley Kathleen M. , Robbins Kelly , Rao Rekha , Balusu Ramesh , Navenot Jean-Marc , Wang Zi-Xuan , Ustun Celalettin , Chong Daniel G. , Atadja Peter , Fujii Nobutaka , Peiper Stephen C. , Bhalla Kapil 
Published: September 2010

Abstract
Stromal cell derived factor-1 (SDF-1 or CXCL12) and its receptor CXCR4 are involved in the directional homing to the bone marrow niches and in peripheral mobilization of normal and transformed hematopoietic stem and myeloid progenitor cells. Elevated CXCR4 expression confers poor prognosis, whereas inhibition of CXCR4 signaling overcomes stroma-mediated chemoresistance in acute myeloid leukemia (AML). Here, we demonstrate that treatment with the pan-histone deacetylase inhibitor panobinostat (PS) depleted the mRNA and protein levels of CXCR4 in the cultured and primary AML cells. PS-induced acetylation of the heat shock protein (hsp) 90 reduced the chaperone association between CXCR4 and hsp90, directing CXCR4 to degradation by the 20S proteasome. PS treatment also depleted G protein–coupled receptor kinase 3, as well as attenuated the phosphorylation of AKT and ERK1/2 in AML cells, which was not affected by cotreatment with CXCL12. Compared with each agent alone, cotreatment with PS and CXCR4 antagonist AMD3100 or FC-131 synergistically induced apoptosis of cultured and primary AML cells. PS and FC-131 exerted more lethal effects on primary AML versus normal CD34+ bone marrow progenitor cells. These findings support the rationale to test the in vivo efficacy of PS in enhancing the lethal effects of CXCR4 antagonists against AML cells.
p21Waf1 expression is regulated by nuclear intermediate filament vimentin in neuroblastoma
Authors: Mergui Xénia , Puiffe Marie-Line , Valteau-Couanet Dominique , Lipinski Marc , Bénard Jean , Amor-Guéret Mounira 
Published: September 2010

Abstract

Background
Human neuroblastoma (NB) cell lines may present with either one of the so-called S-and N-subtypes. We have previously reported a strong correlation between protein expression levels of vimentin, an S-subtype marker, and the p21Waf1 cyclin-dependent kinase inhibitor. We here investigated whether this correlation extend to the mRNA level in NB cell lines as well as in patients' tumors. We also further explored the relationship between expression of vimentin and p21, by asking whether vimentin could regulate p21 expression.

Methods
Vimentin and p21 mRNA levels in NB cell lines as well as in patients' tumors (n = 77) were quantified using Q-PCR. Q-PCR data obtained from tumors of high risk NB patients (n = 40) were analyzed in relation with the overall survival using the Log-rank Kaplan-Meier estimation. siRNA-mediated depletion or overexpression of vimentin in highly or low expressing vimentin cell lines, respectively, followed by protein expression and promoter activation assays were used to assess the role of vimentin in modulating p21 expression.

Results
We extend the significant correlation between vimentin and p21 expression to the mRNA level in NB cell lines as well as in patients' tumors. Overall survival analysis from Q-PCR data obtained from tumors of high risk patients suggests that lower levels of p21 expression could be associated with a poorer outcome. Our data additionally indicate that the correlation observed between p21 and vimentin expression levels results from p21 transcriptional activity being regulated by vimentin. Indeed, downregulating vimentin resulted in a significant decrease in p21 mRNA and protein expression as well as in p21 promoter activity. Conversely, overexpressing vimentin triggered an increase in p21 promoter activity in cells with a nuclear expression of vimentin.

Conclusion
Our results suggest that p21 mRNA tumor expression level could represent a refined prognostic factor for high risk NB patients. Our data also show that vimentin regulates p21 transcription; this is the first demonstration of a gene regulating function for this type III-intermediate filament.
Normal Cortisol Response on Low-Dose Synacthen (1 μg) Test in Children with Prader Willi Syndrome
Authors: Nyunt Ohn , Cotterill Andrew M. , Archbold Sinead M. , Wu Joyce Y. , Leong Gary M. , Verge Charles F. , Crock Patricia A. , Ambler Geoffrey R. , Hofman Paul , Harris Mark 
Published: September 2010

Introduction: It has been postulated that central adrenal insufficiency (CAI), resulting from hypothalamic dysfunction, may contribute to the increased unexplained death rates in Prader Willi syndrome (PWS). A study using the overnight metyrapone test reported a 60% prevalence of CAI in children with PWS. We used a low-dose Synacthen test to screen for CAI in children with PWS.
Methods: We studied 41 children with genetic diagnosis of PWS [20 males; mean age, 7.68 (±5.23) yr] in five pediatric endocrinology centers in Australasia. All participants were randomly selected, and none had a history of Addisonian crisis. Ten of the cohort were receiving sex hormone therapy, 19 were receiving GH, and four were receiving T4. Their mean body mass index z-score was +1.48 (±1.68). Baseline morning ACTH and cortisol levels were measured, followed by iv administration of 1 μg Synacthen. Post-Synacthen cortisol levels were measured at 30 min, and a cortisol level above 500 nmol/liter was considered normal.
Results: The mean baseline ACTH and cortisol were 15 (±14) ng/liter and 223 (±116) nmol/liter, respectively. The mean 30-min plasma cortisol was 690 (±114) nmol/liter, and the average increase from baseline was 201%.
Conclusions: Our result suggests that CAI is rare in children with PWS.
Chemical characterization of tin-lead glazed ceramics from Aragon (Spain) by neutron activation analysis
Authors: Inanez Javier G. , Speakman R. J. , Buxeda i Garrigós Jaume , Glascock M. D. 
Published: September 2010

Abstract
Majolica pottery was the most characteristic tableware produced in Spain during the Medieval and Renaissance periods. A study of the three main production centers in the historical region of Aragon during Middle Ages and Renaissance was conducted on a set of 71 samples. The samples were analyzed by instrumental neutron activation analysis (INAA), and the resulting data were interpreted using an array of multivariate statistical procedures. Our results show a clear discrimination among different production centers allowing a reliable provenance attribution of ceramic sherds from the Aragonese workshops.
Analysis of municipal waste dump soil using a low power reactor: a study by instrumental neutron activation analysis
Authors: Waheed S. , Siddique N. 
Published: September 2010

Abstract
Soil samples from an open waste dump site and a background sample from a site located beside Kashmir Highway near sector H-11, Islamabad, were analyzed for 36 major, minor and trace elements. Suitable instrumental neutron activation analysis (INAA) methodology was developed through optimization of irradiation, cooling and counting protocols using a 27 kW low power miniature neutron source reactor (MNSR). Large variations in the composition of soil samples taken from the dump site were observed and it was found that most of the elements including Al, Ca, Ti, As, Br, Ce, Co, Cs, Ga, Hf, Mn, Nd, La, Sb, Sc, Sm, Sn, Ta, Tb Th, V, Yb, Zn, Eu, Se, and Zr, in the background soil lie in the concentration range of the dump soil sample. However, Fe, K, Mg, Na, Ba, Rb, Sr, U, Zn, Hg and Sb were found to be in higher amounts in the dump site samples. To validate the developed INAA methodology and to ensure the accuracy and precision of the results, IAEA Lake Sediment (SL-1) and IAEA Soil-7 (S-7) were analyzed as control materials.