bluuebunny/crossref_metadata_2025_split huggingface dataset:
Astaxanthin decreased oxidative stress and inflammation and enhanced immune response in humans
Authors: Park Jean Soon , Chyun Jong Hee , Kim Yoo Kyung , Line Larry L , Chew Boon P 
Published: March 2010

AbstractBackgroundAstaxanthin modulates immune response, inhibits cancer cell growth, reduces bacterial load and gastric inflammation, and protects against UVA-induced oxidative stress inin vitroand rodent models. Similar clinical studies in humans are unavailable. Our objective is to study the action of dietary astaxanthin in modulating immune response, oxidative status and inflammation in young healthy adult female human subjects.MethodsParticipants (averaged 21.5 yr) received 0, 2, or 8 mg astaxanthin (n = 14/diet) daily for 8 wk in a randomized double-blind, placebo-controlled study. Immune response was assessed on wk 0, 4 and 8, and tuberculin test performed on wk 8.ResultsPlasma astaxanthin increased (P< 0.01) dose-dependently after 4 or 8 wk of supplementation. Astaxanthin decreased a DNA damage biomarker after 4 wk but did not affect lipid peroxidation. Plasma C-reactive protein concentration was lower (P < 0.05) on wk 8 in subjects given 2 mg astaxanthin. Dietary astaxanthin stimulated mitogen-induced lymphoproliferation, increased natural killer cell cytotoxic activity, and increased total T and B cell subpopulations, but did not influence populations of Thelper, Tcytotoxicor natural killer cells. A higher percentage of leukocytes expressed the LFA-1 marker in subjects given 2 mg astaxanthin on wk 8. Subjects fed 2 mg astaxanthin had a higher tuberculin response than unsupplemented subjects. There was no difference in TNF and IL-2 concentrations, but plasma IFN-γ and IL-6 increased on wk 8 in subjects given 8 mg astaxanthin.ConclusionTherefore, dietary astaxanthin decreases a DNA damage biomarker and acute phase protein, and enhances immune response in young healthy females.
Evaluation of a pig femoral head osteonecrosis model
Authors: Zhang Ping , Liang Yun , Kim Harry , Yokota Hiroki 
Published: March 2010

Abstract

Background
A major cause of osteonecrosis of the femoral head is interruption of a blood supply to the proximal femur. In order to evaluate blood circulation and pathogenetic alterations, a pig femoral head osteonecrosis model was examined to address whether ligature of the femoral neck (vasculature deprivation) induces a reduction of blood circulation in the femoral head, and whether transphyseal vessels exist for communications between the epiphysis and the metaphysis. We also tested the hypothesis that the vessels surrounding the femoral neck and the ligamentum teres represent the primary source of blood flow to the femoral head.

Methods
Avascular osteonecrosis of the femoral head was induced in Yorkshire pigs by transecting the ligamentum teres and placing two ligatures around the femoral neck. After heparinized saline infusion and microfil perfusion via the abdominal aorta, blood circulation in the femoral head was evaluated by optical and CT imaging.

Results
An angiogram of the microfil casted sample allowed identification of the major blood vessels to the proximal femur including the iliac, common femoral, superficial femoral, deep femoral and circumflex arteries. Optical imaging in the femoral neck showed that a microfil stained vessel network was visible in control sections but less noticeable in necrotic sections. CT images showed a lack of microfil staining in the epiphysis. Furthermore, no transphyseal vessels were observed to link the epiphysis to the metaphysis.

Conclusion
Optical and CT imaging analyses revealed that in this present pig model the ligatures around the femoral neck were the primary cause of induction of avascular osteonecrosis. Since the vessels surrounding the femoral neck are comprised of the branches of the medial and the lateral femoral circumflex vessels, together with the extracapsular arterial ring and the lateral epiphyseal arteries, augmentation of blood circulation in those arteries will improve pathogenetic alterations in the necrotic femoral head. Our pig model can be used for further femoral head osteonecrosis studies.
The effect of the combination of acids and tannin in diet on the performance and selected biochemical, haematological and antioxidant enzyme parameters in grower pigs
Authors: Štukelj Marina , Valenčak Zdravko , Krsnik Mladen , Svete Alenka Nemec 
Published: March 2010

AbstractBackgroundThe abolition of in-feed antibiotics or chemotherapeutics as growth promoters have stimulated the swine industry to look for alternatives such as organic acids, botanicals, probiotics and tannin. The objective of the present study was to compare the effects of a combination of acids and tannin with diet with organic acids and diet without growth promoters on the growth performance and selected biochemical, haematological and antioxidant enzyme parameters in grower pigs. Tannin is more natural and cheaper but possibly with the same effectiveness as organic acids with regard to growth performance.MethodsThirty-six 7 week old grower pigs, divided into three equal groups, were used in a three week feeding trial. Group I was fed basal diet, group II basal diet with added organic acids and group III basal diet with added organic and inorganic acids and tannin. Pigs were weighed before and after feeding and observed daily. Blood was collected before and after the feeding trial for the determination of selected biochemical, haematological and antioxidant enzyme parameters. One-way ANOVA was used to assess any diet related changes of all the parameters. Paired t-test was used to evaluate changes of blood parameters individually in each group of growers before and after feeding.ResultsNo clinical health problems related to diet were noted during the three week feeding trial. The average daily gain (ADG) and selected blood parameters were not affected by the addition to basal diet of either acids and tannin or of organic acids alone. Selected blood parameters remained within the reference range before and after the feeding trial, with the exception of total serum proteins that were below the lower value of reference range at both times. The significant changes (paired t-test) observed in individual groups before and after the feeding trial are related to the growth of pigs.ConclusionDiet with acids and tannin did not improve the growth performance of grower pigs but had no deleterious effects on selected blood parameters. The possibility of beneficial effects of adding acids and tannin in diets on growth performance over a longer period, however, could not be excluded.
Mural granulosa cell gene expression associated with oocyte developmental competence
Authors: Jiang Jin-Yi , Xiong Huiling , Cao Mingju , Xia Xuhua , Sirard Marc-Andre , Tsang Benjamin K 
Published: March 2010

Abstract

Background
Ovarian follicle development is a complex process. Paracrine interactions between somatic and germ cells are critical for normal follicular development and oocyte maturation. Studies have suggested that the health and function of the granulosa and cumulus cells may be reflective of the health status of the enclosed oocyte. The objective of the present study is to assess, using an in vivo immature rat model, gene expression profile in granulosa cells, which may be linked to the developmental competence of the oocyte. We hypothesized that expression of specific genes in granulosa cells may be correlated with the developmental competence of the oocyte.

Methods
Immature rats were injected with eCG and 24 h thereafter with anti-eCG antibody to induce follicular atresia or with pre-immune serum to stimulate follicle development. A high percentage (30-50%, normal developmental competence, NDC) of oocytes from eCG/pre-immune serum group developed to term after embryo transfer compared to those from eCG/anti-eCG (0%, poor developmental competence, PDC). Gene expression profiles of mural granulosa cells from the above oocyte-collected follicles were assessed by Affymetrix rat whole genome array.

Results
The result showed that twelve genes were up-regulated, while one gene was down-regulated more than 1.5 folds in the NDC group compared with those in the PDC group. Gene ontology classification showed that the up-regulated genes included lysyl oxidase (Lox) and nerve growth factor receptor associated protein 1 (Ngfrap1), which are important in the regulation of protein-lysine 6-oxidase activity, and in apoptosis induction, respectively. The down-regulated genes included glycoprotein-4-beta galactosyltransferase 2 (Ggbt2), which is involved in the regulation of extracellular matrix organization and biogenesis.

Conclusions
The data in the present study demonstrate a close association between specific gene expression in mural granulosa cells and the developmental competence of oocytes. This finding suggests that the most differentially expressed gene, lysyl oxidase, may be a candidate biomarker of oocyte health and useful for the selection of good quality oocytes for assisted reproduction.
Barriers to initiation of antiretroviral treatment in rural and urban areas of Zambia: a cross‐sectional study of cost, stigma, and perceptions about ART
Authors: Fox Matthew P , Mazimba Arthur , Seidenberg Phil , Crooks Denise , Sikateyo Bornwell , Rosen Sydney 
Published: March 2010

BackgroundWhile the number of HIV‐positive patients on antiretroviral therapy (ART) in resource‐limited settings has increased dramatically, some patients eligible for treatment do not initiate ART even when it is available to them. Understanding why patients opt out of care, or are unable to opt in, is important to achieving the goal of universal access.MethodsWe conducted a cross‐sectional survey among 400 patients on ART (those who were able to access care) and 400 patients accessing home‐based care (HBC), but who had not initiated ART (either they were not able to, or chose not to, access care) in two rural and two urban sites in Zambia to identify barriers to and facilitators of ART uptake.ResultsHBC patients were 50% more likely to report that it would be very difficult to get to the ART clinic than those on ART (RR: 1.48; 95% CI: 1.21‐1.82). Stigma was common in all areas, with 54% of HBC patients, but only 15% of ART patients, being afraid to go to the clinic (RR: 3.61; 95% CI: 3.12‐4.18). Cost barriers differed by location: urban HBC patients were three times more likely to report needing to pay to travel to the clinic than those on ART (RR: 2.84; 95% CI: 2.02‐3.98) and 10 times more likely to believe they would need to pay a fee at the clinic (RR: 9.50; 95% CI: 2.24‐40.3). In rural areas, HBC subjects were more likely to report needing to pay non‐transport costs to attend the clinic than those on ART (RR: 4.52; 95% CI: 1.91‐10.7). HBC patients were twice as likely as ART patients to report not having enough food to take ART being a concern (27% vs. 13%, RR: 2.03; 95% CI: 1.71‐2.41), regardless of location and gender.ConclusionsPatients in home‐based care for HIV/AIDS who never initiated ART perceived greater financial and logistical barriers to seeking HIV care and had more negative perceptions about the benefits of the treatment. Future efforts to expand access to antiretroviral care should consider ways to reduce these barriers in order to encourage more of those medically eligible for antiretrovirals to initiate care.
Effect of Glucagon-Like Peptide-1 on α- and β-Cell Function in C-Peptide-Negative Type 1 Diabetic Patients
Authors: Kielgast Urd , Asmar Meena , Madsbad Sten , Holst Jens J. 
Published: March 2010

Abstract
Context: The mechanism by which glucagon-like peptide-1 (GLP-1) suppresses glucagon secretion is uncertain, and it is not determined whether endogenous insulin is a necessary factor for this effect.
Objective: To characterize the α- and β-cell responses to GLP-1 in type 1 diabetic patients without residual β-cell function.
Methods: Nine type 1 diabetic patients, classified as C-peptide negative by a glucagon test, were clamped at plasma glucose of 20 mmol/liter for 90 min with arginine infusion at time 45 min and concomitant infusion of GLP-1 (1.2 pmol/kg · min) or saline.
Results: Infusion with GLP-1 increased C-peptide concentration just above the detection limit of 33 pmol/liter in one patient, but C-peptide remained immeasurable in all other patients. In the eight remaining patients, total area under the curve of glucagon was significantly decreased with GLP-1 compared with saline: 485 ± 72 vs. 760 ± 97 pmol/liter · min (P &lt; 0.001). In addition, GLP-1 decreased the arginine-stimulated glucagon release (incremental AUC of 103 ± 21 and 137 ± 16 pmol/liter · min, with GLP-1 and saline, respectively, P &lt; 0.05).
Conclusions: In type 1 diabetic patients without endogenous insulin secretion, GLP-1 decreases the glucagon secretion as well as the arginine-induced glucagon response during hyperglycemia. GLP-1 induced endogenous insulin secretion in one of nine type 1 diabetic patients previously classified as being without endogenous insulin secretion.
Regulation of ERRα Gene Expression by Estrogen Receptor Agonists and Antagonists in SKBR3 Breast Cancer Cells: Differential Molecular Mechanisms Mediated by G Protein-Coupled Receptor GPR30/GPER-1
Authors: Li Yin , Birnbaumer Lutz , Teng Christina T. 
Published: March 2010

AbstractIn selected tissues and cell lines, 17β-estradiol (E2) regulates the expression of estrogen-related receptor α (ERRα), a member of the orphan nuclear receptor family. This effect is thought to be mediated by the estrogen receptor α (ERα). However in the ERα- and ERβ-negative SKBR3 breast cancer cell line, physiological levels of E2 also stimulate ERRα expression. Here, we explored the molecular mechanism that mediates estrogen action in ER-negative breast cancer cells. We observed that E2, the ERα agonist, as well as the ERα antagonists ICI 182,780 and tamoxifen (TAM), a selective ER modulator, stimulate the transcriptional activity of the ERRα gene and increase the production of ERRα protein in SKBR3 cells. Moreover, the ERRα downstream target genes expression and cellular proliferation are also increased. We show further that the G protein-coupled receptor GPR30/GPER-1 (GPER-1) mediates these effects. The GPER-1 specific ligand G-1 mimics the actions of E2, ICI 182,780, and TAM on ERRα expression, and changing the levels of GPER-1 mRNA by overexpression or small interfering RNA knockdown affected the expression of ERRα accordingly. Utilizing inhibitors, we delineate a different downstream pathway for ER agonist and ER antagonist-triggered signaling through GPER-1. We also find differential histone acetylation and transcription factor recruitment at distinct nucleosomes of the ERRα promoter, depending on whether the cells are activated with E2 or with ER antagonists. These findings provide insight into the molecular mechanisms of GPER-1/ERRα-mediated signaling and may be relevant to what happens in breast cancer cells escaping inhibitory control by TAM.
FOXL2 Interacts with Steroidogenic Factor-1 (SF-1) and Represses SF-1-Induced CYP17 Transcription in Granulosa Cells
Authors: Park Mira , Shin Eunkyoung , Won Miae , Kim Jae-Hong , Go Hayoung , Kim Hyun-Lee , Ko Jeong-Jae , Lee Kangseok , Bae Jeehyeon 
Published: March 2010

Abstract
Mutations in FOXL2 are responsible for blepharophimosis-ptosis-epicanthus inversus syndrome (BPES) type I, in which affected women exhibit premature ovarian failure. FOXL2-null mice showed defects in granulosa cell development during folliculogenesis. We screened a rat ovarian yeast two-hybrid cDNA library to identify FOXL2-interacting proteins and found steroidogenic factor-1 (SF-1). Here, we show that human FOXL2 and SF-1 proteins interact in human granulosa cells and that FOXL2 negatively regulates the transcriptional activation of a steroidogenic enzyme, CYP17, by SF-1. Furthermore, FOXL2 mutants found in blepharophimosis-ptosis-epicanthus inversus syndrome type I patients lost the ability to repress CYP17 induction mediated by SF-1. Chromatin immunoprecipitation and EMSA results further revealed that FOXL2 inhibited the binding of SF-1 to the CYP17 promoter, whereas the FOXL2 mutants failed to block this interaction. Therefore, this study identifies a novel regulatory role for FOXL2 on a key steroidogenic enzyme and provides a possible mechanism by which mutations in FOXL2 disrupt normal ovarian follicle development.
Highly Porous and Stable Coordination Polymers as Water Sorption Materials
Authors: Akiyama George , Matsuda Ryotaro , Kitagawa Susumu 
Published: March 2010

Abstract
Three MIL-100-type porous coordination polymers were synthesized using different types of anions and their water sorption properties were investigated. All of the compounds adsorbed huge amounts of water (0.6 g g−1) at a moderate humidity (P/P0 &lt; 0.6), and one of the compounds showed the same adsorption property even after two thousand replicate water sorption tests. In addition, the pressure of water adsorption was found to be controlled by counter anions incorporated in the structure, which was attributed to the hydration energy of the anions.
Templateless Synthesis of Nanoporous Gold Sponge with Surface-enhanced Raman Scattering Activity
Authors: Ataee-Esfahani Hamed , Fukata Naoki , Yamauchi Yusuke 
Published: March 2010

Abstract
We demonstrate templateless synthesis of nanoporous gold (Au) sponge by chemical reduction of Au species in aqueous solution. The obtained Au nanosponges exhibit surface-enhanced Raman scattering activity.
Tactical and Operational Planning of Scheduled Maintenance for Per-Seat, On-Demand Air Transportation
Authors: Keysan Gizem , Nemhauser George L. , Savelsbergh Martin W. P. 
Published: March 2010

Advances in aviation technology including the development of relatively cheap, very light jets and the possibility of free-flight have led to the realization of a per-seat, on-demand air transportation business that operates without a published flight schedule. One of the decision problems this business faces is planning the scheduled maintenance that has to be done periodically for safety reasons. Careful planning of scheduled maintenance in this dynamic business environment is required to ensure safe and efficient operations. In this paper, we address both tactical and operational planning for scheduled maintenance of per-seat, on-demand air transportation. At the tactical level, we determine the daily maintenance capacities. As the fleet size grows over time, decisions pertaining to when and how much to increase maintenance capacity are made. At the operational level, we assign itineraries to jets and determine the jets to be maintained on a daily basis. We further embed operational maintenance planning into a framework that captures the interaction between maintenance decisions and daily flight scheduling.
Congestion Pricing for Schedule-Based Transit Networks
Authors: Hamdouch Younes , Lawphongpanich Siriphong 
Published: March 2010

In this paper, we develop models for adjusting or setting fares on a transit system to encourage passengers to choose travel strategies that lead to the least travel delay for the entire system. In our problem setting, these fares vary with time of day. Similar to the one used to reduce congestion on vehicular traffic networks, our goal is to adjust or set fares so that a user equilibrium solution under the new fares yields the least delay or is system optimal. On the other hand, pricing frameworks for traffic networks such as marginal cost pricing do not readily apply because the travel delay in transit systems involves factors different from those in vehicular traffic and cannot be expressed in closed functional forms. The models herein are schedule based and account for loading priorities and individual vehicle capacities explicitly. Differences among the proposed models are illustrated with a small network.
Contamination of the Norepinephrine Prodrug Droxidopa by Dihydroxyphenylacetaldehyde
Authors: Holmes Courtney , Whittaker Noel , Heredia-Moya Jorge , Goldstein David S 
Published: March 2010

AbstractBackground: l-Threo-3,4-dihydroxyphenylserine (L-DOPS, droxidopa) is a norepinephrine (NE) prodrug under development to treat orthostatic hypotension. 3,4-Dihydroxyphenylacetaldehyde (DOPAL), an endogenous catecholaldehyde produced by enzymatic oxidative deamination of dopamine, is toxic to catecholaminergic neurons. Based on the observation of increasing plasma DOPAL after oral administration of L-DOPS to a patient, we examined whether other subjects also had DOPAL in their plasma after droxidopa administration, and whether droxidopa is contaminated with DOPAL.Methods: Thirteen subjects took 400 mg droxidopa orally. We sampled venous blood at baseline and 1, 2, 3, 6, 24, and 48 h after drug administration and assayed L-DOPS, NE, and DOPAL by use of liquid chromatography with electrochemical detection (LC-ED). Droxidopa in acidic solution (20:80 mixture of 0.04 mol/L phosphoric acid:0.20 mol/L acetic acid) was vacuum centrifuged for 1 h at 30 °C and then assayed by LC-ED.Results: Droxidopa contained 0.01% DOPAL. At 6 h after droxidopa, all subjects had detectable DOPAL in plasma (1.89 nmol/L, P = 0.0001). Across the sampling times, plasma DOPAL correlated with plasma L-DOPS (r = 0.996). The mean increment in plasma DOPAL was more than 4 times that in plasma NE (0.39 nmol/L). In 2 patients with Parkinson disease and orthostatic hypotension, DOPAL was detected in plasma at baseline (0.12 nmol/L) and increased by about 70-fold after droxidopa. Vacuum concentration of droxidopa in the acid solution converted L-DOPS to DOPAL completely.Conclusions: Droxidopa is contaminated with DOPAL. After oral droxidopa administration, DOPAL is detected in plasma of humans. Droxidopa is susceptible to extensive nonenzymatic conversion to DOPAL.
Remembering the SIEV X: Who Cares for the Bodies of the Stateless, Lost at Sea?
Authors: Gibbings Beth 
Published: March 2010

Abstract
The SIEV X was a tiny fishing vessel traveling from Indonesia to Australia in 2001, carrying around four hundred people seeking asylum after fleeing from the warfare and persecution predominantly in Iraq and Afghanistan. Many were women and children trying to enter Australia to join fathers and husbands already granted refugee status but not allowed to bring in family members because of new Australian laws on “Temporary Protection Visas.” Of these, 353 drowned when the boat sank in international waters. The conservative Australian government denied responsibility, using the event in an election campaign to play on fears about illegal entry and border defense in the Islamophobic climate in the aftermath of 9/11. Yet many everyday Australians eventually became involved in a collaborative design process to create a memorial to those asylum seekers. This article discusses the debates around memorials for those lost at sea, and particularly for those who might be portrayed as enemies or “illegal immigrants” whose coming threatens national borders. It identifies the conditions under which the campaign to commemorate those who died on the SIEV X moved from being a minority interest to become a cause so widely supported by Australians across the country that the memorial was eventually erected in the heart of the national capital.