bluuebunny/crossref_metadata_2025_split huggingface dataset:
Decoding intelligence via symmetry and asymmetry
Authors: Fu Jianjing , Hsiao Ching-an 
Published: May 2024

AbstractHumans use pictures to model the world. The structure of a picture maps to mind space to form a concept. When an internal structure matches the corresponding external structure, an observation functions. Whether effective or not, the observation is self-consistent. In epistemology, people often differ from each other in terms of whether a concept is probabilistic or certain. Based on the effect of the presented IG and pull anti algorithm, we attempt to provide a comprehensive answer to this problem. Using the characters of hidden structures, we explain the difference between the macro and micro levels and the same difference between semantics and probability. In addition, the importance of attention is highlighted through the combination of symmetry and asymmetry included and the mechanism of chaos and collapse revealed in the presented model. Because the subject is involved in the expression of the object, representationalism is not complete. However, people undoubtedly reach a consensus based on the objectivity of the representation. Finally, we suggest that emotions could be used to regulate cognition.
Bio-synthesis, purification and structural analysis of Cyclosporine-A produced by Tolypocladium inflatum with valorization of agro-industrial wastes
Authors: Falah Fereshteh , Samie Ali , Mortazavi Seyed Ali , Danesh Abolghasem , Yazdi Farideh Tabatabaei , Ramezani Mohammad 
Published: May 2024

AbstractCyclosporine A (CyA) holds significant importance as a strategic immunosuppressive drug for organ transplant patients. In this study, we aimed to produce pure and cost-effective Cyclosporine A (CyA) by fermenting a culture medium containing dairy sludge, using Tolypocladium inflatum PTCC 5253. Following the fermentation stage, ethyl acetate extraction and fast protein liquid chromatography were employed for sample purification. The initial evaluation of the effectiveness of CyA obtained from these processes was performed through bioassay, wherein the antimicrobial clear zone diameter was found to be larger compared to the sample obtained from the fermentation culture. The concentration of CyA was determined using high-performance liquid chromatography, yielding values of 334 mg/L, 456 mg/L, and 578 mg/L for the fermented, extracted, and purified samples, respectively. Further analysis utilizing liquid chromatography tandem mass spectrometry (LC/MS/MS) confirmed a purity of 91.9% and proper agreement with the standard sample based on the ion intensity of Z/m 1205. To validate the structure of CyA, nuclear magnetic resonance spectroscopy, Fourier-transform infrared (FT-IR), and Raman spectroscopy were employed. X-ray diffraction and differential scanning calorimetry analyses demonstrated that the purified CyA exhibited a crystal structure similar to the standard sample, characterized by two broad peaks at 2θ = 9° and 20°, and comparable glass transition temperatures (57–68 °C for the purified sample; 53–64 °C for the standard sample). Dynamic light scattering analysis confirmed a uniform particle size distribution in both the purified and standard samples. The zeta potentials of the purified and standard samples were determined to be − 25.8 ± 0.16 and − 23.63 ± 0.12 mV, respectively. Our results demonstrate that dairy sludge can serve as a suitable culture medium for the production of (CyA).
Research on provincial water resources carrying capacity and coordinated development in China based on combined weighting TOPSIS model
Authors: Zhu Qianying , Cao Yi 
Published: May 2024

AbstractWith the continuous development of the economy and society, along with the sustained population growth, the issue of water resources carrying capacity in China has attracted increasing attention. This paper constructs a model for evaluating the provincial water resources carrying capacity in China from four dimensions: water, economy, society, and ecology. Utilizing this model, we analyze the spatiotemporal variations in water resources carrying capacity among 31 provinces in China from 2005 to 2021. Additionally, we delve into the coupling coordination and influencing factors of water resources carrying capacity. The study reveals an overall increasing trend in China’s water resources carrying capacity index, with the ecological indicator exhibiting the most significant growth while the water resources sub-indicator lags behind. There are notable regional differences, with higher water resources carrying capacity observed in the eastern coastal areas and relatively lower capacity in the western regions. The ecological criterion becomes a core factor constraining water resources carrying capacity from 2005 to 2015, gradually giving way to the prominence of the social criterion since 2015. The coordination degree is relatively higher in the eastern regions, more scattered in the western regions, and relatively stable in the central regions. Based on the research findings, a series of recommendations are proposed, including strengthening environmental protection policies, optimizing water resources management mechanisms, improving water use efficiency, and promoting economic structural diversification. These suggestions aim to facilitate the sustainable development of water resources carrying capacity in China.
Pulsed electric field induces exocytosis and overexpression of MAGE antigens in melanoma
Authors: Szlasa Wojciech , Sauer Natalia , Baczyńska Dagmara , Ziętek Marcin , Haczkiewicz-Leśniak Katarzyna , Karpiński Paweł , Fleszar Mariusz , Fortuna Paulina , Kulus Michał J. , Piotrowska Aleksandra , Kmiecik Alicja , Barańska Agnieszka , Michel Olga , Novickij Vitalij , Tarek Mounir , Kasperkiewicz Paulina , Dzięgiel Piotr , Podhorska-Okołów Marzenna , Saczko Jolanta , Kulbacka Julita 
Published: May 2024

AbstractNanosecond pulsed electric field (nsPEF) has emerged as a promising approach for inducing cell death in melanoma, either as a standalone treatment or in combination with chemotherapeutics. However, to date, there has been a shortage of studies exploring the impact of nsPEF on the expression of cancer-specific molecules. In this investigation, we sought to assess the effects of nsPEF on melanoma-specific MAGE (Melanoma Antigen Gene Protein Family) expression. To achieve this, melanoma cells were exposed to nsPEF with parameters set at 8 kV/cm, 200 ns duration, 100 pulses, and a frequency of 10 kHz. We also aimed to comprehensively describe the consequences of this electric field on melanoma cells' invasion and proliferation potential. Our findings reveal that following exposure to nsPEF, melanoma cells release microvesicles containing MAGE antigens, leading to a simultaneous increase in the expression and mRNA content of membrane-associated antigens such as MAGE-A1. Notably, we observed an unexpected increase in the expression of PD-1 as well. While we did not observe significant differences in the cells' proliferation or invasion potential, a remarkable alteration in the cells' metabolomic and lipidomic profiles towards a less aggressive phenotype was evident. Furthermore, we validated these results using ex vivo tissue cultures and 3D melanoma culture models. Our study demonstrates that nsPEF can elevate the expression of membrane-associated proteins, including melanoma-specific antigens. The mechanism underlying the overexpression of MAGE antigens involves the initial release of microvesicles containing MAGE antigens, followed by a gradual increase in mRNA levels, ultimately resulting in elevated expression of MAGE antigens post-experiment. These findings shed light on a novel method for modulating cancer cells to overexpress cancer-specific molecules, thereby potentially enhancing their sensitivity to targeted anticancer therapy.
Flow regimes identification of air water counter current flow in vertical annulus using differential pressure signals and machine learning
Authors: Cao Feng , Dang Ruirong , Dang Bo , Zheng Huifeng , Ji Anzhao , Chen Zhanjun , Zhao Jiaxuan , Sun Zhimeng 
Published: May 2024

AbstractThis study investigates the gas–liquid two-phase counter-current flow through a vertical annulus, a phenomenon prevalent across numerous industrial fields. The presence of an inner pipe and varying degrees of eccentricity between the inner and outer pipes often blur the clear demarcation of flow regime boundaries. To address this, we designed a vertical annulus with adjustable eccentricity (outer and inner diameters of 125 mm and 75 mm, respectively). We conducted gas–liquid counter-current flow experiments under specific conditions: gas superficial velocity ranging from 0.06 to 5.04 m/s, liquid superficial velocity from 0.01 to 0.25 m/s, and five levels of eccentricity (e = 0, 0.25, 0.5, 0.75, 1). We collected differential pressure data at two distinct height distances (DP1: 50 mm and DP2: 1000 mm). We used vectors, composed of both the probability density functions (PDFs) of the differential pressure signals and the power spectral density (PSD) reduced via Principal Component Analysis, as features. Using the CFDP clustering algorithm—based on local density—we clustered the flow regimes of the experimental data, thereby achieving an objective and consistent identification of the flow regime of gas–liquid two-phase counter-current flow in a vertical annulus. Our analysis reveals that for DP1, the main differences in the PSD of various flow regimes occur within the 0.5–1 Hz range. Among the three flow regimes involved, the slug flow exhibits the highest power intensity, followed by the bubbly flow, with the churn flow having the least. In terms of differential pressure distribution, the bubbly and churn flows have a concentrated distribution, while the slug flow is more dispersed. For DP2, the PSD differences primarily exist within the 0.5–2 Hz range. The churn flow has the highest power intensity, followed by the slug flow, with the bubbly flow being the weakest. Here, the bubbly flow's differential pressure distribution is concentrated, while the slug and churn flows are more dispersed. Based on the results of the flow regime classification, we generated a flow regime map and analyzed the influence of annulus eccentricity on the flow regime. We found that in most cases, pipe eccentricity does not significantly affect the flow regime. However, in the transition region—such as the bubbly to slug flow transition zone—flows with medium eccentricity values (e = 0.5, 0.75) are more likely to transition to slug flow. We compared the visual recognition results of flow regimes with the clustering results. 4.04% of the total samples showed different results from visual recognition and clustering, primarily located in the flow regime transition area. Since visually distinguishing flow regimes in these areas is typically challenging, our methodology offers an objective classification approach for gas–liquid two-phase counter-current flow in a vertical annulus.
Blood perfusion with polymyxin B immobilized columns in patients with COVID-19 requiring oxygen therapy
Authors: Katagiri Daisuke , Tsukada Akinari , Izumi Shinyu , Shimizu Yosuke , Terada-Hirashima Junko , Uemura Yukari , Kusaba Yusaku , Takasaki Jin , Takoi Hiroyuki , Tamura-Nakano Miwa , Hojo Masayuki , Takano Hideki , Noiri Eisei , Abe Shinji , Azuma Arata , Sugiyama Haruhito 
Published: May 2024

AbstractExtracorporeal blood purification with polymyxin B immobilized fiber column direct hemoperfusion (PMX-DHP), is reported to be effective in treating COVID-19 pneumonitis with oxygen demand. This multicenter prospective study evaluated the efficacy and safety of PMX-DHP in oxygen-requiring patients with COVID-19 admitted between September 28, 2020, and March 31, 2022. The primary endpoint was the percentage of clinical improvement 15 days after treatment. The secondary endpoint was the percentage of worsened disease status. Data from the COVID-19 patient registry were used for the synthetic control group. The improvement rate on Day 15 did not differ between PMX-treated patients and controls; however, the deterioration rate was 0.38 times lower in the PMX-treated group, and the death rates on Day 29 were 0 and 11.1% in the PMX-treated and control groups, respectively. The PMX group showed a 0.73 times higher likelihood for reduced intensive care demand, as 16.7% of PMX-treated patients and 22.8% of controls worsened. After treatment blood oxygenation improved, urinary β2-microglobulin and liver-type fatty acid-binding protein showed significant decreases, and IL-6 decreased once during treatment but did not persist. In this study, PMX treatment effectively prevented the worsening of COVID-19 pathology, accompanied by improved oxygenation. PMX treatment to remove activated cells may effectively improve patient outcomes.
Construction of Vero cell-adapted rabies vaccine strain by five amino acid substitutions in HEP-Flury strain
Authors: Harada Michiko , Matsuu Aya , Park Eun-Sil , Inoue Yusuke , Uda Akihiko , Kaku Yoshihiro , Okutani Akiko , Posadas-Herrera Guillermo , Ishijima Keita , Inoue Satoshi , Maeda Ken 
Published: May 2024

AbstractRabies virus (RABV) causes fatal neurological disease. Pre-exposure prophylaxis (PrEP) and post-exposure prophylaxis (PEP) using inactivated-virus vaccines are the most effective measures to prevent rabies. In Japan, HEP-Flury, the viral strain, used as a human rabies vaccine, has historically been propagated in primary fibroblast cells derived from chicken embryos. In the present study, to reduce the cost and labor of vaccine production, we sought to adapt the original HEP-Flury (HEP) to Vero cells. HEP was repeatedly passaged in Vero cells to generate ten- (HEP-10V) and thirty-passaged (HEP-30V) strains. Both HEP-10V and HEP-30V grew significantly better than HEP in Vero cells, with virulence and antigenicity similar to HEP. Comparison of the complete genomes with HEP revealed three non-synonymous mutations in HEP-10V and four additional non-synonymous mutations in HEP-30V. Comparison among 18 recombinant HEP strains constructed by reverse genetics and vesicular stomatitis viruses pseudotyped with RABV glycoproteins indicated that the substitution P(L115H) in the phosphoprotein and G(S15R) in the glycoprotein improved viral propagation in HEP-10V, while in HEP-30V, G(V164E), G(L183P), and G(A286V) in the glycoprotein enhanced entry into Vero cells. The obtained recombinant RABV strain, rHEP-PG4 strain, with these five substitutions, is a strong candidate for production of human rabies vaccine.
Perception of experience influences altruism and perception of agency influences trust in human–machine interactions
Authors: Oudah Mayada , Makovi Kinga , Gray Kurt , Battu Balaraju , Rahwan Talal 
Published: May 2024

AbstractAs robots become increasingly integrated into social economic interactions, it becomes crucial to understand how people perceive a robot’s mind. It has been argued that minds are perceived along two dimensions: experience, i.e., the ability to feel, and agency, i.e., the ability to act and take responsibility for one’s actions. However, the influence of these perceived dimensions on human–machine interactions, particularly those involving altruism and trust, remains unknown. We hypothesize that the perception of experience influences altruism, while the perception of agency influences trust. To test these hypotheses, we pair participants with bot partners in a dictator game (to measure altruism) and a trust game (to measure trust) while varying the bots’ perceived experience and agency, either by manipulating the degree to which the bot resembles humans, or by manipulating the description of the bots’ ability to feel and exercise self-control. The results demonstrate that the money transferred in the dictator game is influenced by the perceived experience, while the money transferred in the trust game is influenced by the perceived agency, thereby confirming our hypotheses. More broadly, our findings support the specificity of the mind hypothesis: Perceptions of different dimensions of the mind lead to different kinds of social behavior.
Microbiome dysbiosis in patients with chronic endometritis and Clostridium tyrobutyricum ameliorates chronic endometritis in mice
Authors: Liu Jiujiu , Tang Xiaorong , Chen Lei , Zhang Yue , Gao Jinfang , Wang Aiming 
Published: May 2024

AbstractChronic endometritis is associated with the imbalance of female reproductive tract microbiota and pathogenic microbial infection. This study aimed to identify the specific changes in the endometrial microbiome in patients with endometritis and to explore how Clostridium tyrobutyricum (C.t) influences the progression of endometritis in mice for further elucidating endometritis pathogenesis. For this purpose, endometrial tissues from 100 participants were collected and divided into positive, weakly positive, and negative groups based on CD138 levels, while endometrial microbiome differences were detected and analyzed using 16S rRNA gene sequencing. Staphylococcus aureus (S. aureus)-induced endometritis mouse model was established, followed by treatment with C.t, and inflammatory response, epithelial barrier, and TLR4/NF-κB pathway were evaluated. Results showed that α- and β-diversity was significantly lower in the positive group compared with the weakly positive or negative groups, where the negative group had more unique operational taxonomic units. The abundance of Proteobacteria was found to be increased, while that of Actinobacteria, Firmicutes, and Bacteroidetes was found to be reduced in the positive group, while the area under the curve value was found to be 0.664. Furthermore, C.t treatment resulted in the alleviation of S. aureus-induced inflammatory response, epithelial barrier damage, and activation of the TLR4/NF-κB pathway in mice. Clinical samples analysis revealed that the diversity and abundance of microbiota were altered in patients with endometritis having positive CD138 levels, while mechanistic investigations revealed C.t alleviated S. aureus-induced endometritis by inactivating TLR4/NF-κB pathway. The findings of this study are envisaged to provide a diagnostic and therapeutic potential of microbiota in endometritis.
Laparoscopic distal gastrectomy skill evaluation from video: a new artificial intelligence-based instrument identification system
Authors: Matsumoto Shiro , Kawahira Hiroshi , Fukata Kyohei , Doi Yasunori , Kobayashi Nao , Hosoya Yoshinori , Sata Naohiro 
Published: May 2024

AbstractThe advent of Artificial Intelligence (AI)-based object detection technology has made identification of position coordinates of surgical instruments from videos possible. This study aimed to find kinematic differences by surgical skill level. An AI algorithm was developed to identify X and Y coordinates of surgical instrument tips accurately from video. Kinematic analysis including fluctuation analysis was performed on 18 laparoscopic distal gastrectomy videos from three expert and three novice surgeons (3 videos/surgeon, 11.6 h, 1,254,010 frames). Analysis showed the expert surgeon cohort moved more efficiently and regularly, with significantly less operation time and total travel distance. Instrument tip movement did not differ in velocity, acceleration, or jerk between skill levels. The evaluation index of fluctuation β was significantly higher in experts. ROC curve cutoff value at 1.4 determined sensitivity and specificity of 77.8% for experts and novices. Despite the small sample, this study suggests AI-based object detection with fluctuation analysis is promising because skill evaluation can be calculated in real time with potential for peri-operational evaluation.
Regulation of TSC2 lysosome translocation and mitochondrial turnover by TSC2 acetylation status
Authors: Marqués Patricia , Burillo Jesús , González-Blanco Carlos , Jiménez Beatriz , García Gema , García-Aguilar Ana , Iglesias-Fortes Sarai , Lockwood Ángela , Guillén Carlos 
Published: May 2024

AbstractSirtuin1 (SIRT1) activity decreases the tuberous sclerosis complex 2 (TSC2) lysine acetylation status, inhibiting the mechanistic target of rapamycin complex 1 (mTORC1) signalling and concomitantly, activating autophagy. This study analyzes the role of TSC2 acetylation levels in its translocation to the lysosome and the mitochondrial turnover in both mouse embryonic fibroblast (MEF) and in mouse insulinoma cells (MIN6) as a model of pancreatic β cells. Resveratrol (RESV), an activator of SIRT1 activity, promotes TSC2 deacetylation and its translocation to the lysosome, inhibiting mTORC1 activity. An improvement in mitochondrial turnover was also observed in cells treated with RESV, associated with an increase in the fissioned mitochondria, positive autophagic and mitophagic fluxes and an enhancement of mitochondrial biogenesis. This study proves that TSC2 in its deacetylated form is essential for regulating mTORC1 signalling and the maintenance of the mitochondrial quality control, which is involved in the homeostasis of pancreatic beta cells and prevents from several metabolic disorders such as Type 2 Diabetes Mellitus.
Complexities of peroxyacetyl nitrate photochemistry and its control strategies in contrasting environments in the Pearl River Delta region
Authors: Liu Tao , Wang Yu , Cai Huang , Wang Hao , Zhang Chunlin , Chen Jun , Dai Yan , Zhao Wenlong , Li Jiangyong , Gong Daocheng , Chen Duohong , Zhai Yuhong , Zhou Yan , Liao Tong , Wang Boguang 
Published: May 2024

AbstractPeroxyacetyl nitrate (PAN) is a hazardous secondary pollutant and a nitrogen reservoir in the troposphere, while comprehensive studies on its spatiotemporal distribution, chemical sources, and control strategies are limited. This study addressed these issues based on 1-year multi-site observations in the Pearl River Delta region, showing a pattern of higher PAN levels in the cold season, particularly in coastal areas. Moreover, in situ modeling indicated a net photochemical PAN formation in the warm season, while PAN was dominated by regional transport in the cold season (i.e., rural and coastal areas), releasing NO2 and PA radicals and promoting ozone (O3) formation. In addition, the feasibility of joint control of PAN and O3 was confirmed by their isopleth diagrams. C7–C9 aromatics and C4–C5 alkenes, largely from vehicle exhaust and solvent usage, were identified as the predominant contributors to PAN formation in this region. Overall, this study deepens our understanding of PAN chemistry and provides valuable insights into its control measures.