bluuebunny/crossref_metadata_2025_split huggingface dataset:
Voltammetric, UV–Vis Spectrometric and Fluorescence Study of the Interaction of Ractopamine and DNA with the Aid of Multivariate Curve Resolution‐Alternating Least Squares
Authors: Ni Yongnian , Wang Yingxia , Kokot Serge 
Published: July 2010

AbstractInteraction of a steroid drug, Ractopamine (RAC), and DNA was investigated by electroanalysis‐differential pulse and cyclic voltammetry (DPV and CV), and UV‐vis and fluorescence spectroscopy. DPV showed that RAC intercalated with DNA, and CV indicated that the reaction mechanism of RAC and dsDNA involved irreversible oxidation with the loss of two H+ and a transfer of two electrons. Reaction binding parameters were obtained. Pure spectra of RAC, DNA and the RAC‐DNA complex, and their concentrations were extracted by multivariate curve resolution‐alternating least squares method (MCR‐ALS). Concentration profiles indicated quantitatively the course of the reaction.
Complete Elucidation of Electrode Reaction Mechanisms by Using Differential Pulse Polarography
Authors: Mellado José Miguel Rodríguez , Amaro Rafael Rodríguez 
Published: July 2010

AbstractBy exploring the different parameters of the technique, it is shown that Differential Pulse Polarography (DPP) can be used for the elucidation of the reaction mechanisms of the electrochemical processes (with the evident exception of the product and intermediate identification). So, the type of transport towards or from the electrode can be identified from the dependence of the intensities with the pulse amplitude, the electrochemical reaction order with respect to the electroactive species from the shape of the polarogram, the type of rate‐determining step from the dependence of the peak potentials on the pulse duration, the electrochemical reaction order with respect to other species, such as the H+ion, from the dependence of the peak potentials on their concentrations etc.
Use of a Carbon‐Ink Microelectrode Array for Signal Enhancement in Microchip Electrophoresis with Electrochemical Detection
Authors: Mecker Laura C. , Filla Laura A. , Martin R. Scott 
Published: July 2010

AbstractIn this communication, we demonstrate that a carbon ink microelectrode array, where the electrodes are held at the same potential, affords significant signal enhancement in microchip electrophoresis with amperometric detection. The ability to fabricate an array of carbon ink microelectrodes with a palladium decoupler was demonstrated and the resulting electrodes were integrated with a valving microchip design. The use of an 8 electrode array led to a significant improvement in the limits of detection at the expense of separation resolution due to the increased detection zone size. It is also shown that microdialysis sampling can be integrated with the microchip device and a multianalyte separation achieved.
Identifying causes for certain types of electrically initiated fires in residential circuits
Authors: Shea John J. 
Published: July 2010

AbstractWhile there are many circumstances that can lead to residential electrical fires, it is useful and practical to identify and document potential hazardous conditions and provide physical explanations of electrically initiated fires. Failure modes for a variety of parallel and series arc faults are described along with circuit breaker response, electrical data, photos, and video frames to explain the conditions that led to a hazardous condition. This work identifies some of the various conditions that can create overheated hazardous conditions and shows how wire insulation is commonly the first to ignite. Conditions explored include bundled extension cords under rugs, abused NM‐B at a load center entrance, broken wires and loose connections. Physical phenomena investigated include glowing connections, series and parallel arcing faults. Low and high current arc energies are discussed and compared to the combustible energy of volatile gases produced from wire insulation to illustrate the wide difference in the arc energy compared to chemical combustion energy from the decomposed PVC. Electrical wire insulation properties were also measured on various wire types to show the effects of thermal aging. Hardness of the wire insulation and cracking of the insulation was used as a measure of insulation lifetime at 140∘C. Copyright © 2010 John Wiley & Sons, Ltd.
Random aggregation with applications in high‐frequency finance
Authors: Tsay Ruey S. , Yeh Jin‐Huei 
Published: July 2010

AbstractIn this paper we consider properties of random aggregation in time series analysis. For application, we focus on the problem of estimating the high‐frequency beta of an asset return when the returns are subject to the effects of market microstructure. Specifically, we study the correlation between intraday log returns of two assets. Our investigation starts with the effect of non‐synchronous trading on intraday log returns when the underlying return series follows a stationary time series model. This is a random aggregation problem in time series analysis. We also study the effect of non‐synchronous trading on the covariance of two asset returns. To overcome the impact of non‐synchronous trading, we use Markov chain Monte Carlo methods to recover the underlying log return series based on the observed intraday data. We then define a high‐frequency beta based on the recovered log return series and propose an efficient method to estimate the measure. We apply the proposed analysis to many mid‐ or small‐cap stocks using the Trade and Quote Data of the New York Stock Exchange, and discuss implications of the results obtained. Copyright © 2010 John Wiley & Sons, Ltd.
Spinal cord injury induces differential expression of the profibrotic semaphorin 7A in the developing and mature glial scar
Authors: Kopp Marcel A. , Brommer Benedikt , Gatzemeier Nicole , Schwab Jan M. , Prüss Harald 
Published: July 2010

AbstractSemaphorin 7A (Sema7A) is involved in the formation of the central nervous system during development by operating axon guidance and neuronal migration. We investigated the expression of the TGFβ‐inducible Sema7A following spinal cord injury (SCI). After SCI, Sema7A+ cells accumulated specifically in lesion areas resulting in significantly enhanced Sema7A expression at the injury site (P < 0.0001). During the first days lesional Sema7A expression was confined to neurons, ballooned neurite fibers/retraction bulbs, and endothelial cells. At day 7, we observed Sema7A expression by components of the glial scar, such as reactive astrocytes and pronounced extracellular Sema7A deposition. In the direct perilesional rim, Sema7A+ astrocytes coexpressed the activation‐associated intermediate filament vimentin. In the injured spinal cord, numbers of Sema7A+ cells reached maximum levels at day 14. The restricted accumulation of Sema7A+ reactive astrocytes and Sema7A deposition in fibronectin+ extracellular matrix territories suggests a participation of the fibrostimulatory Sema7A in the developing and maturating scar following SCI. In addition, Sema7A appears to be marker a for astrocyte activation. © 2010 Wiley‐Liss, Inc.
Ultrasonographic screening for occult thyroid cancer
Authors: Yuen Anthony Po Wing , Ho Ambrose Chung Wai , Wong Birgitta Yee Hang 
Published: July 2010

AbstractBackground.It has been shown that occult thyroid carcinoma can be identified in about 10% of thyroid glands in autopsy. The purpose of this study was to evaluate the prevalence of asymptomatic thyroid carcinoma in the Hong Kong Chinese population by ultrasonographic screening.Methods.Volunteer subjects without symptoms of thyroid disease were recruited for ultrasonographic screening for thyroid cancer. A total of 1140 subjects were recruited in this study.Results.Thyroid nodules were found in 45% subjects (511 of 1140); the mean age was 48 years. Ultrasonographic‐guided aspiration cytology was performed in 258 subjects. Twenty‐six subjects (2.3%) had cytologic findings suspicious of malignancy. Fourteen subjects (1.2%) had pathologic diagnosis of thyroid cancer, including 13 papillary carcinomas and 1 follicular carcinoma.Conclusion.A high incidence of occult thyroid cancer could be identified on ultrasonographic screening. Subjects could benefit with early diagnosis with either early intervention or close monitoring. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Sustained NF‐kappaB activity in chronic lymphocytic leukemia is independent of genetic and epigenetic alterations in the TNFAIP3 (A20) locus
Authors: Frenzel Lukas P. , Claus Rainer , Plume Nadine , Schwamb Janine , Konermann Carolin , Pallasch Christian P. , Claasen Julia , Brinker Reinhild , Wollnik Bernd , Plass Christoph , Wendtner Clemens‐Martin 
Published: July 2010

AbstractInappropriate nuclear factor (NF) κB activity is one major hallmark of B‐cell malignancies and chronic lymphocytic leukemia (CLL). NFκB‐dependent genes are involved in antiapoptosis, cell proliferation and metastasis and are responsible for survival and proliferation of tumors. However, the mechanisms of NFκB activity in CLL still need to be elucidated. Previously, we identified translocations in a region on chromosome 6q that encodes tumor necrosis factor alpha‐induced protein 3, which is a key player in negative feedback loop regulation of NFκB. Inactivation of this ubiquitin‐editing enzyme is involved in immunopathologies and in tumorigenesis. Frequent mutations in the A20 locus—leading to sustained NFκB activity—could be shown to play a dominant role in development of different B‐cell malignancies. To check if A20 is involved in upregulation of NFκB activity in CLL, we sequenced Exons 2–9 of the A20 gene in 55 CLL DNA samples. Furthermore, we determined the methylation status of the promoter region in 63 CLL DNA samples and compared to 10 control DNAs of B cells from healthy donors. Contrary to reports from other B‐cell malignancies, the A20 region showed neither mutations nor aberrant DNA methylation. Moreover, its expression could be confirmed by immunoblotting and showing comparable results to healthy B cells. These results indicate that malignant development in CLL differs from most of other B‐cell malignancies, which show frequent inactivation of A20.
Bone material properties in actively bone-forming trabeculae in postmenopausal women with osteoporosis after three years of treatment with once-yearly Zoledronic acid
Authors: Gamsjaeger Sonja , Buchinger Birgit , Zwettler Elizabeth , Recker Robert , Black Dennis , Gasser Juerg A , Eriksen Erik F , Klaushofer Klaus , Paschalis Eleftherios P 
Published: July 2010

Abstract
Zoledronic acid (ZOL), a third-generation aminobisphosphonate, showed pronounced antifracture efficacy in a phase III clinical trial [Health Outcomes and Reduced Incidence with Zoledronic Acid Once Yearly—Pivotal Fracture Trial (HORIZON-PFT)] when administered yearly (5-mg infusions of ZOL), producing significant reductions in morphometric vertebral, clinical vertebral, hip, and nonvertebral fractures by 70%, 77%, 41%, and 25%, respectively, over a 3-year period. The purpose of this study was to analyze the biopsies obtained during the HORIZON clinical trial (152 patients, 82 ZOL and 70 placebo) by means of Raman microspectroscopy (a vibrational spectroscopic technique capable of analyzing undecalcified bone tissue with a spatial resolution of approximately 0.6 µm) to determine the effect of ZOL therapy on bone material properties (in particular mineral/matrix ratio, lamellar organization, carbonate and proteoglycan (based on spectral identification of glycosaminoglycan) content, and mineral maturity/crystallinity) at similar tissue age (based on the presence of tetracycline double labels). The results indicated that while ZOL administration increased the mineral/matrix ratio compared with placebo, it also resulted in mineral crystallites with a quality profile (based on carbonate content and maturity/crystallinity characteristics) of younger (with respect to tissue age) bone. Since the comparisons between ZOL- and placebo-treated patients were performed at similar tissue age at actively forming bone surfaces, these results suggest that ZOL may be exerting an effect on bone matrix formation in addition to its well-established antiresorptive effect, thereby contributing to its antifracture efficacy. © 2011 American Society for Bone and Mineral Research.
QM/MM method for metal–organic interfaces
Authors: Sushko Maria L. , Sushko Peter V. , Abarenkov Igor V. , Shluger Alexander L. 
Published: July 2010

AbstractWe present a QM/MM method for modeling metal/organic interfaces, which incorporates contributions from long‐range electron correlation, characteristic to metals and non‐bonded interactions in organic systems. This method can be used to study structurally irregular systems. We apply the method to model finite size domains of self‐assembled monolayers on the gold (111) surface and discuss the influence of boundary effects on the electrostatic and electronic properties of these systems. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
NUPACK: Analysis and design of nucleic acid systems
Authors: Zadeh Joseph N. , Steenberg Conrad D. , Bois Justin S. , Wolfe Brian R. , Pierce Marshall B. , Khan Asif R. , Dirks Robert M. , Pierce Niles A. 
Published: July 2010

AbstractThe Nucleic Acid Package (NUPACK) is a growing software suite for the analysis and design of nucleic acid systems. The NUPACK web server (http://www.nupack.org) currently enables:
Analysis: thermodynamic analysis of dilute solutions of interacting nucleic acid strands.
Design: sequence design for complexes of nucleic acid strands intended to adopt a target secondary structure at equilibrium.
Utilities: evaluation, display, and annotation of equilibrium properties of a complex of nucleic acid strands.
NUPACK algorithms are formulated in terms of nucleic acid secondary structure. In most cases, pseudoknots are excluded from the structural ensemble. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
Isomerization of stilbene using enforced geometry optimization
Authors: Baker Jon , Wolinski Krzysztof 
Published: July 2010

AbstractUsing our recently proposed quantum chemical model to simulate the effect of external forces acting on a molecule (Wolinski and Baker, Mol Phys 2009, 107, 2403), which we subsequently termed enforced geometry optimization (EGO), we investigate structural isomerism in C14H12, starting from cis‐stilbene. By applying an external force to pairs of carbon atoms, one from each “half” of the molecule, we have generated 10 different structural isomers. Each was characterized as a minimum by vibrational analysis. Not only can EGO generate potentially new, metastable isomers it can also provide good initial guesses for transition states connecting the starting and final structures, thus giving an estimate of the stability of the new isomers to rearrangement back to the starting material. In addition to the new isomers, we provide a full set of vibrational fundamentals for cis‐ and trans‐stilbene and 4a,4b‐dihydrophenanthrene. The agreement with experimental assignments is excellent, with mean average deviations for the stilbenes of 5.0 cm−1 or less. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
Synthesis and characterization of novel color chemosensors based on azo dyes for possible application in opioid pharmacology
Authors: Genady Afaf R. 
Published: July 2010

Abstract
magnified imageAn applicable strategy of chemical labeling of morphine (M) and 6‐acetyl morphine (6‐AM) using diazonium salts is described. M or 6‐AM was coupled with aryl diazoinum salts to give morphine azo dyes. The coupling of the diazotized 4,4′‐diaminostilbene with M or 6‐AM in ratio 1:2 gave stilbene‐based azo dyes containing two M or 6‐AM units, respectively. Diazotization of 5‐(p‐aminophenyl)‐10,15,20‐triphenylporphyrin and subsequent azo coupling of the diazoniun salt with M and with 6‐AM was our route to get highly fluorescent morphine dyes. The resulting dyes can exist in two possible tautomeric structures, azo and hydrazone, stabilized to a significant extent by intramolecular H‐bonding. It was shown that these dyes exists predominantly or exclusively in their hydrazone form. This conclusion is drawn from the lack of a distinct band in the 380–420 nm region of the absorption spectra (a region in which the long wavelength absorption band for the azo‐form is observed), together with results of NMR studies in deuterated DMSO. The tautomeric properties of the compounds were judged by density functional calculations at the B3LYP/6‐31G* and B3LYP/6‐31G** levels. The analysis of spiked compounds in human urine samples was studied by capillary electrophoresis (CE) with UV‐fluorescence photo‐diode array detectors. J. Heterocyclic Chem., (2010).
Synthesis of pyrano[3,4‐c]chromene skeleton via CuI‐mediated domino Knoevenagel hetero‐Diels‐Alder reaction
Authors: Mollazadeh Marjan , Khoshkholgh Malihe Javan , Balalaie Saeed , Rominger Frank , Bijanzadeh Hamid Reza 
Published: July 2010

Abstract
magnified image A new strategy involving domino Knoevenagel hetero‐Diels‐Alder reaction is described for the preparation of pyrano[3,4‐c]chromenes scaffold. Reaction of O‐propargylated salicylaldehyde with benzoylacetonitrile or Meldrum's acid in the presence of CuI and diammonium hydrogen phosphate affords pyrano[3,4‐c]chromenes with good yields and high bond‐forming efficiency. J. Heterocyclic Chem., (2010).
The mood stabilizer valproic acid improves defective neurite formation caused by charcot‐marie‐tooth disease‐associated mutant Rab7 through the JNK signaling pathway
Authors: Yamauchi Junji , Torii Tomohiro , Kusakawa Shinji , Sanbe Atsushi , Nakamura Kazuaki , Takashima Shou , Hamasaki Hajime , Kawaguchi Shogo , Miyamoto Yuki , Tanoue Akito 
Published: July 2010

AbstractCharcot‐Marie‐Tooth (CMT) disease is the most frequent peripheral neuropathy affecting the Schwann cells and neurons. CMT disease type 2 (CMT2) neuropathies are characterized by peripheral nerve aberrance. Four missense mutations of Rab7, a small GTPase of the Rab family involved in intracellular vesicular trafficking, are associated with the CMT2B phenotype. Despite a growing body of evidence concerning the gene structures responsible for genetically heterogenous CMT2B and other CMT2 neuropathies, little is known about the in vitro neuropathy model and how CMT2B‐associated mutation‐caused aberrant neuritogenesis is properly reversed. Here, we show that valproic acid (VPA), a classical mood‐stabilizing drug, improves defective neurite formation in N1E‐115 neuroblastoma cells regardless of which CMT2B‐associated Rab7 mutant protein is expressed. The effect is mediated by c‐Jun N‐terminal kinase (JNK) signaling, but not by deacetylase inhibition activity of VPA itself. Furthermore, VPA has similar effects in dorsal root ganglion (DRG) neurons expressing any of the four mutant Rab7 proteins. Thus, VPA has a previously unknown potential to improve defective neuritogenesis associated with CMT2B in vitro, indicating that JNK should be a potential therapeutic target for treatments aimed at improving neuritogenesis. © 2010 Wiley‐Liss, Inc.
ADHD in a representative sample of the Brazilian population: estimated prevalence and comparative adequacy of criteria between adolescents and adults according to the item response theory
Authors: Polanczyk Guilherme , Laranjeira Ronaldo , Zaleski Marcos , Pinsky Ilana , Caetano Raul , Rohde Luis Augusto 
Published: July 2010

AbstractBackground: Attention deficit hyperactivity disorder (ADHD) is a clinically significant disorder in adulthood, but current diagnostic criteria and instruments do not seem to adequately capture the complexity of the disorder in this developmental phase. Accordingly, there are limited data on the proportion of adults affected by the disorder, specially in developing countries.Method: We assessed a representative household sample of the Brazilian population for ADHD with the Adult ADHD Self‐report Scale (ASRS) Screener, and evaluated the instrument according to the Rasch model of item response theory.Results: The sample was comprised by 3007 individuals, and the overal prevalence of positive screeners for ADHD was 5.8% [95% confidence interval (CI), 4.8–7.0]. Rasch analyses revealed the misfitt of the overall sample to expectations of the model. The evaluation of the sample stratified by age revealed that data for adolescents showed a signficant fittnes to the model expectations, while items completed by adults were not adequated.Conclusions: The lack of fitness to the model for adult respondents challenges the possibility of a linear transformation of the ordinal data into interval measures and the utilization of parametric analyses of data. This result suggests that diagnostic criteria and instruments for adult ADHD must take into account a developmental perspective. Moreover, it calls for further evaluation of currently employed research methods in light of modern theories of psychometrics. Copyright © 2010 John Wiley & Sons, Ltd.
The effects of anticalcification treatments and hydration on the molecular dynamics of bovine pericardium collagen as revealed by 13C solid‐state NMR
Authors: deAzevedo E. R. , Ayrosa A. M. I. B. , Faria G. C. , Cervantes H. J. , Huster D. , Bonagamba T. J. , Pitombo R. N. M. , Rabbani S. R. 
Published: July 2010

AbstractThis article describes a solid‐state NMR (SSNMR) investigation of the influence of hydration and chemical cross‐linking on the molecular dynamics of the constituents of the bovine pericardium (BP) tissues and its relation to the mechanical properties of the tissue. Samples of natural phenetylamine‐diepoxide (DE)‐ and glutaraldehyde (GL)‐fixed BP were investigated by 13C cross‐polarization SSNMR to probe the dynamics of the collagen, and the results were correlated to the mechanical properties of the tissues, probed by dynamical mechanical analysis. For samples of natural BP, the NMR results show that the higher the hydration level the more pronounced the molecular dynamics of the collagen backbone and sidechains, decreasing the tissue's elastic modulus. In contrast, in DE‐ and GL‐treated samples, the collagen molecules are more rigid, and the hydration seems to be less effective in increasing the collagen molecular dynamics and reducing the mechanical strength of the samples. This is mostly attributed to the presence of cross‐links between the collagen plates, which renders the collagen mobility less dependent on the water absorption in chemically treated samples. Copyright © 2010 John Wiley & Sons, Ltd.
Structure elucidation and NMR assignments of two unusual monoterpene indole alkaloids from Psychotria stachyoides
Authors: Pimenta Antonia Torres Ávila , Braz‐Filho Raimundo , Delprete Piero Giuseppe , de Souza Elnatan Bezerra , Silveira Edilberto Rocha , Lima Mary Anne Sousa 
Published: July 2010

AbstractTwo unusual monoterpene indole alkaloids, stachyoside (1) and nor‐methyl‐23‐oxo‐correantoside (2), have been isolated from the aerial parts of Psychotria stachyoides. The structural elucidation of both compounds was performed by the aid of HRESIMS, FT‐IR, and 1D‐ and 2D‐NMR techniques including COSY, HSQC, HMBC, and NOESY. Copyright © 2010 John Wiley & Sons, Ltd.