bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhancing iron content and growth of cucumber seedlings with MgFe-LDHs under low-temperature stress
Authors: Wu Hongyang , Wan Xiaoyang , Niu Jiefei , Cao Yidan , Wang Shufang , Zhang Yu , Guo Yayu , Xu Huimin , Xue Xian , Yao Jun , Zhu Cuifang , Li Yang , Li Qiang , Lu Tao , Yu Hongjun , Jiang Weijie 
Published: May 2024

AbstractThe development of cost-effective and eco-friendly fertilizers is crucial for enhancing iron (Fe) uptake in crops and can help alleviate dietary Fe deficiencies, especially in populations with limited access to meat. This study focused on the application of MgFe-layered double hydroxide nanoparticles (MgFe-LDHs) as a potential solution. We successfully synthesized and characterized MgFe-LDHs and observed that 1–10 mg/L MgFe-LDHs improved cucumber seed germination and water uptake. Notably, the application of 10 mg/L MgFe-LDHs to roots significantly increased the seedling emergence rate and growth under low-temperature stress. The application of 10 mg/L MgFe-LDHs during sowing increased the root length, lateral root number, root fresh weight, aboveground fresh weight, and hypocotyl length under low-temperature stress. A comprehensive analysis integrating plant physiology, nutrition, and transcriptomics suggested that MgFe-LDHs improve cold tolerance by upregulating SA to stimulate CsFAD3 expression, elevating GA3 levels for enhanced nitrogen metabolism and protein synthesis, and reducing levels of ABA and JA to support seedling emergence rate and growth, along with increasing the expression and activity of peroxidase genes. SEM and FTIR further confirmed the adsorption of MgFe-LDHs onto the root hairs in the mature zone of the root apex. Remarkably, MgFe-LDHs application led to a 46% increase (p < 0.05) in the Fe content within cucumber seedlings, a phenomenon not observed with comparable iron salt solutions, suggesting that the nanocrystalline nature of MgFe-LDHs enhances their absorption efficiency in plants. Additionally, MgFe-LDHs significantly increased the nitrogen (N) content of the seedlings by 12% (p < 0.05), promoting nitrogen fixation in the cucumber seedlings. These results pave the way for the development and use of LDH-based Fe fertilizers.
Graphical Abstract
Decentralised same day test and treatment of hepatitis C levering existing peer support networks among men who inject drugs: feasibility and effectiveness
Authors: Rajkumar Nalinikanta , Khumukcham Lokeshwar Singh , Thangjam Dhabali , Singh Surender , Khwairakpam Giten , Shilton Sonjelle , Goel Amit 
Published: May 2024

Abstract
Background
Prevalence of hepatitis C virus (HCV) infection among people who inject drugs in the state of Manipur, India, is 43%; however, access to care is poor. We piloted a Community-led and comprehensive hepatitis care model that included same-day HCV treatment at drug treatment centres.

Methods
Screening was conducted through venipuncture samples collected by community peer PWID, using HCV antibody (HCV Ab) rapid screening and hepatitis B virus (HBV) surface antigen (HBsAg) rapid diagnostic tests. Reactive HCV Ab samples were tested for HCV RNA using near point-of-care Truenat® HCV on Truelab® Quattro. Eligible HCV RNA-positive participants were treated on the same day using direct-acting antivirals and followed for sustained virologic response (SVR). HBsAg-negative participants received rapid HBV vaccination regimen while those positive for HBsAg were tested for DNA and referred for treatment.

Results
Between November 2021 and August 2022, 643 individuals were approached and 503 consented and were screened. All screened were males with history of injection drug use, and a median age of 27 years (IQR 23–32). Of the 241 (47.9%) HCV Ab reactive all underwent RNA testing and 156 (64.7%) were RNA detectable. Of those with viraemia, 155 (99.4%) were initiated on treatment with 153 (98.1%) on same day, with 2 (1.2%) HBsAg positive and waiting for HBV DNA results. Among those 153, median time from HCV Ab screening to treatment was 6 h 38 min (IQR 5 h 42 min–8 h 23 min). In total 155 (100%) completed HCV treatment, of those 148 (95.5%) completed SVR testing and 130 (87.8%) achieved SVR12. 27 (5%) participants were HBsAg-positive, 3 (11.1%) were also living with HCV viraemia; 443 (97.6%) were eligible for vaccination and 436 (98.4%) received all 3 vaccine doses.

Conclusion
Community-led hepatitis care incorporating same day “test and treat” for HCV was feasible and effective. HBV screening identified a large proportion who were unvaccinated. Peer support extended resulted in ensuring compliance to care and treatment cascade and completing all the three doses of HBV vaccination. As the screening, diagnostics infrastructure and vaccine are available in most countries with national viral hepatitis programs also in place, our model can be adapted or replicated to progress towards global elimination targets.
Fibroblast growth factor pathway promotes glycolysis by activating LDHA and suppressing LDHB in a STAT1-dependent manner in prostate cancer
Authors: Ye Yongkang , Yang Fukan , Gu Zhanhao , Li Wenxuan , Yuan Yinjiao , Liu Shaoqian , Zhou Le , Han Bo , Zheng Ruinian , Cao Zhengguo 
Published: May 2024

Abstract
Background
The initiation of fibroblast growth factor 1 (FGF1) expression coincident with the decrease of FGF2 expression is a well-documented event in prostate cancer (PCa) progression. Lactate dehydrogenase A (LDHA) and LDHB are essential metabolic products that promote tumor growth. However, the relationship between FGF1/FGF2 and LDHA/B-mediated glycolysis in PCa progression is not reported. Thus, we aimed to explore whether FGF1/2 could regulate LDHA and LDHB to promote glycolysis and explored the involved signaling pathway in PCa progression.

Methods
In vitro studies used RT‒qPCR, Western blot, CCK-8 assays, and flow cytometry to analyze gene and protein expression, cell viability, apoptosis, and cell cycle in PCa cell lines. Glycolysis was assessed by measuring glucose consumption, lactate production, and extracellular acidification rate (ECAR). For in vivo studies, a xenograft mouse model of PCa was established and treated with an FGF pathway inhibitor, and tumor growth was monitored.

Results
FGF1, FGF2, and LDHA were expressed at high levels in PCa cells, while LDHB expression was low. FGF1/2 positively modulated LDHA and negatively modulated LDHB in PCa cells. The depletion of FGF1, FGF2, or LDHA reduced cell proliferation, induced cell cycle arrest, and inhibited glycolysis. LDHB overexpression showed similar inhibitory effect on PCa cells. Mechanistically, we found that FGF1/2 positively regulated STAT1 and STAT1 transcriptionally activated LDHA expression while suppressed LDHB expression. Furthermore, the treatment of an FGF pathway inhibitor suppressed PCa tumor growth in mice.

Conclusion
The FGF pathway facilitates glycolysis by activating LDHA and suppressing LDHB in a STAT1-dependent manner in PCa.
NSUN2 affects diabetic retinopathy progression by regulating MUC1 expression through RNA m5C methylation
Authors: Wang Runze , Xue Wei , Kan Feifei , Zhang Huiying , Wang Di , Wang Lei , Wang Jianwen 
Published: May 2024

Abstract
Background
Diabetic retinopathy (DR) is the leading cause of blinding eye disease among working adults and is primarily attributed to the excessive proliferation of microvessels, which leads to vitreous hemorrhage and retinal traction, thereby significantly impairing patient vision. NSUN2-mediated RNA m5C methylation is implicated in various diseases, and in this investigation, we focused on elucidating the impact of NSUN2 on the regulation of the expression of the downstream gene MUC1, specifically through RNA m5C methylation, on the progression of DR.

Method
Utilizing Microarray analysis, we examined patient vitreous fluid to pinpoint potential therapeutic targets for DR. Differential expression of NSUN2 was validated through qRT-PCR, Western blot, and immunofluorescence in human tissue, animal tissue, and cell model of DR. The relationship between NSUN2 and DR was explored in vitro and in vivo through gene knockdown and overexpression. Various techniques, such as MeRIP-qPCR and dot blot, were applied to reveal the downstream targets and mechanism of action of NSUN2.

Results
The levels of both NSUN2 and RNA m5C methylation were significantly elevated in the DR model. Knockdown of NSUN2 mitigated DR lesion formation both in vitro and in vivo. Mechanistically, NSUN2 promoted MUC1 expression by binding to the RNA m5C reader ALYREF. Knockdown of ALYREF resulted in DR lesion alterations similar to those observed with NSUN2 knockdown. Moreover, MUC1 overexpression successfully reversed a series of DR alterations induced by NSUN2 silencing.

Conclusions
NSUN2 regulates the expression of MUC1 through ALYREF-mediated RNA m5C methylation, thereby regulating the progression of DR and providing a new option for the treatment of DR in the future.
Systematic review of the economic evaluation model of assisted reproductive technology
Authors: Si Yuxin , Tan Tao , Pu Kexue 
Published: May 2024

Abstract
Background
With the increasing demand for fertility services, it is urgent to select the most cost-effective assisted reproductive technology (ART) treatment plan and include it in medical insurance. Economic evaluation reports are an important reference for medical insurance negotiation. The aim of this study is to systematically evaluate the economic evaluation research of ART, analyze the existing shortcomings, and provide a reference for the economic evaluation of ART.

Methods
PubMed, EMbase, Web of Science, Cochrane Library and ScienceDirect databases were searched for relevant articles on the economic evaluation of ART. These articles were screened, and their quality was evaluated based on the Comprehensive Health Economics Evaluation Report Standard (CHEERS 2022), and the data on the basic characteristics, model characteristics and other aspects of the included studies were summarized.

Results
One hundred and two related articles were obtained in the preliminary search, but based on the inclusion criteria, 12 studies were used for the analysis, of which nine used the decision tree model. The model parameters were mainly derived from published literature and included retrospective clinical data of patients. Only two studies included direct non-medical and indirect costs in the cost measurement. Live birth rate was used as an outcome indicator in half of the studies.

Conclusion
Suggesting the setting of the threshold range in the field of fertility should be actively discussed, and the monetary value of each live birth is assumed to be in a certain range when the WTP threshold for fertility is uncertain. The range of the parameter sources should be expanded. Direct non-medical and indirect costs should be included in the calculation of costs, and the analysis should be carried out from the perspective of the whole society. In the evaluation of clinical effect, the effectiveness and safety indexes should be selected for a comprehensive evaluation, thereby making the evaluation more comprehensive and reliable. At least subgroup analysis based on age stratification should be considered in the relevant economic evaluation.
Experimental and Numerical Evaluation for Hybrid Reinforced T-Beam with Different Ratios of Recycled Rubberized Concrete
Authors: El-Salakawy Tarik S. , Gamal Amr A. , Sayed Mohamed Essam 
Published: May 2024

AbstractThe use of hybrid GFRP and steel bars as main reinforcement increases the flexural capacity of T-section concrete beams and reduces ductility. Adding recycled rubber to the concrete mix would further enhance the ductility of the hybrid system. Evaluation of the concrete's flexural capacity and ductility is the main goal of the current investigation using normal concrete (NC) and rubberized recycled concrete (RRC). Eight T-beams have been experimentally investigated in this research, two beams were reinforced with steel bars and GFRP bars with zero percentage of crumb rubber (C.R). The remaining beams were reinforced with different combinations of GFRP and steel bars with rubberized concrete mixes with partial substitution of sand with recycled crumb rubber by (0%, 7.5%, 10%, and 12.5% replacements by volume) particle size 1.0 to 2.0 mm. The ductility index for the tested hybrid rubberized T-beams (HRTB) BRH1, BRH3a, BRH5, BRH2, BRH4, and BRH6, were higher than BH1 and BH2 by 28.2%, 35.47%, 65.38%, 23.76%, 30.04%, and 56.95% indicating that increasing the percentage of C.R. has a direct effect on increasing the ductility index. The ultimate failure load for the tested HRTB BRH1, BRH3a, and BRH5, decreased by 11.68%, 14.29%, and 17.47% compared to the hybrid T-beam BH1. The energy dissipation decreased for HRTB BRH1, BRH3a, BRH5, BRH2, BRH4 and BRH6 by 7.88%, 12.36%, 17.17%, 8.12%, 12.96%, and 18.28 compared to hybrid T-beams BH1 and BH2. This indicates that the existence of the very weak C.R. was not able to dissipate the energy properly within the concrete matrix. Good agreement was found between the numerical model and experimental results in terms of crack pattern, ultimate loads and deflections.
Application of machine learning and resistivity measurements for 3D apparent geological modeling in the Yilan plain, Taiwan, at the SW Tip of the Okinawa trough
Authors: Chang Ping-Yu , Puntu Jordi Mahardika , Lin Ding-Jiun , Amania Haiyina Hasbia , Chen Wen-Shan , Lin Andrew Tien-shun 
Published: May 2024

AbstractThis study presents a pioneering investigation into the complex Holocene paleo-morphologies of the Yilan Plain, located at the southwestern edge of the Okinawa Trough. We employed a novel approach that synergized resistivity measurements with machine learning techniques to unlock valuable insights into the geological history, sedimentary patterns, and seismic activity of this dynamic region. Our methodology involved the creation of an Apparent Geological Model (AGM) through the interpolation of inverted resistivity data and the application of supervised machine learning algorithms. Classification criteria, derived from the relationship between resistivity values and sediment types found in nearby boreholes, were developed using the random forest machine-learning method. The resultant 3D resistivity model was transformed into a clay-sand-gravel model, offering a comprehensive depiction of sediment distribution within the Yilan Plain. Notably, our findings revealed distinct sedimentary patterns. Gravel-dominated regions, characterized by resistivity values above 140 Ohm-m, were identified alongside areas dominated by sand and clay sediments. The Carbon-14 dating ages in the sand sediments exhibited remarkable consistency, shedding light on the depositional history of the region. Furthermore, our research unveiled a previously unknown phenomenon of rapid subsidence in the Yilan Plain. Through meticulous analysis and correction for sea-level changes, we estimated an average subsidence rate of approximately 8.5 mm/year. This subsidence was punctuated by abrupt events around 6000–7000 years BP and 2000–3000 years BP, associated with a sudden increase. These events suggested a potential link to prehistoric seismic activity, with variable subsidence rates between episodes hinting at recurrent active seismic periods every 4000–5000 years. In conclusion, our multidisciplinary approach has provided unprecedented insights into the Holocene paleo-morphologies of the Yilan Plain. By combining resistivity measurements, machine learning, and geological analysis, we have enriched our understanding of the region's geological history, sedimentary dynamics, and seismic behavior. These findings not only contribute to the knowledge of Yilan’s past but also offer vital data for future environmental and geological studies in similarly dynamic regions.
Reinforcement of pimobendan with guideline-directed medical therapy may reduce the rehospitalization rates in patients with heart failure: retrospective cohort study
Authors: Iwade Yoshiki , Kubota Yoshiaki , Hayashi Daisuke , Nishino Takuya , Watanabe Yukihiro , Kato Katsuhito , Tara Shuhei , Ise Yuya , Asai Kuniya 
Published: May 2024

Abstract
Background
Pimobendan reportedly improves the subjective symptoms of heart failure. However, evidence of improved prognosis is lacking. This study aimed to determine whether reinforcing guideline-directed medical therapy (GDMT) improved rehospitalization rates for worsening heart failure in patients administered pimobendan.

Methods
A total of 175 patients with heart failure who were urgently admitted to our hospital for worsening heart failure and who received pimobendan between January 2015 and February 2022 were included. Of the 175 patients, 44 were excluded because of in-hospital death at the time of pimobendan induction. The remaining 131 patients were divided into two groups, the reduced ejection fraction (rEF) (n = 93) and non-rEF (n = 38) groups, and further divided into the GDMT-reinforced and non-reinforced groups.

Results
In patients with rEF, the rate of rehospitalization for heart failure was significantly lower in the GDMT-reinforced group than in the non-reinforced group (log-rank test, P = .04). However, the same trend was not observed in the non-rEF group.

Conclusions
Reinforcing GDMT may reduce the heart failure rehospitalization rate in patients with pimobendan administration and rEF. However, multicenter collaborative research is needed.

Trial registration
IRB Approval by the Nippon Medical School Hospital Ethics Committee B-2021-433 (April 10, 2023).
Development and implementation of carbon accounting models and standardization platforms in public institutions
Authors: Bai Yan , Zhang Rui , Yu Bo , Zhang Lan , Guan Jinxin , Ma Yuexin 
Published: May 2024

AbstractPublic institutions, emblematic of public infrastructure, exhibit extensive reach and operational scope, positioning them as vanguards in China’s dual carbon initiatives and serving as exemplars. Electricity and natural gas predominantly fuel the operations of public institutions. Notably, the fixed commute routes and consistent procurement patterns of office personnel yield a standardized energy consumption profile within these entities. Researching carbon emissions related to commuting and evaluating procurement strategies for reducing carbon footprints in public institutions demonstrate a precision-tailored approach. This paper, through an analysis of the energy consumption characteristics, utilization structure of public institutions, and the commuting behaviors and procurement practices of office personnel, establishes a bespoke carbon accounting model specifically designed for public institutions, seamlessly embedded within a comprehensive platform. By providing fundamental methodological frameworks and advanced technological foundations for carbon accounting in public institutions across China, this work propels the nation’s efforts towards carbon peak and ultimately carbon neutrality.
Left ventricular function assessment in Kawasaki disease by two-dimensional global longitudinal systolic strain with automated function imaging
Authors: Moghadam Ehsan Aghaei , Taraz Maryam , Zeinaloo Aliakbar , Majnoon Mohammad Taghi , Zanjani Keyhan Sayadpour , Gorgi Mojtaba 
Published: May 2024

Abstract
Background
Kawasaki disease is an acute febrile vasculitis of childhood mainly affecting children under 4 years of age. In the acute stage of the disease, heart function decreases and gradually returns to normal after treatment. However, subendocardial involvement may persist, which cannot be assessed by M-mode echocardiography. Strain echocardiography is a recently developed technique to assess subendocardial involvement of myocardial deformation. We aimed to study the stratified strain of left ventricular function in a Kawasaki patient at least 6 months after the acute stage of the disease with special conditions for entering the study using two-dimensional speckle-tracking imaging. Between September 2020 and October 2022, 27 healthy children and 27 children with a history of Kawasaki disease more than 6 months ago were evaluated using two-dimensional global longitudinal peak systolic strain with automated function imaging technology.

Results
The mean age of patients was 5.6 years. With M-mode echocardiography, ejection fraction of each group was in the normal range. Mean (± standard deviation) global longitudinal peak strain in four-chamber view of girls with Kawasaki disease was − 23.74 ± 2.77, and that in boys with Kawasaki disease was − 20.93 ± 2.06 (P value = 0.008). GLPS (global longitudinal peak strain) was compared as an overall average and as in a separate segment, which showed significant difference in two comparisons. In our study, a decrease in the function of some cardiac segments is reported. Global longitudinal peak strain in four-chamber view was significantly lower in boys. Comparing different segments, a difference in global left ventricular long-axis strain was found between the two groups. On the other hand, there was a major difference between the two groups in the basal inferolateral, basal anterolateral, and mid-inferolateral, which receives blood from Left Circumflex artery.

Conclusion
Using stain echocardiography to detect continued subendocardial involvement in asymptomatic children with a history of Kawasaki disease for a better understanding of the condition, effective management and follow-up is recommended.
Digital addiction in children with end-stage kidney disease on regular hemodialysis: a cross-sectional study
Authors: Ayoub Doaa Riad , Ramadan Yasmin , Afifi Dina Youssri , Kamel Reham Mohamed 
Published: May 2024

Abstract
Background
Chronic kidney disease has a detrimental impact on the overall quality of life (QoL) in children. The regular visits to doctors, prolonged reliance on medication, frequent hospital stays, and changes in their physical appearance increase their vulnerability to developing psychiatric problems and hinder their daily activities and social interactions, resulting in isolation, poor peer relations, and low self-esteem. These factors may compel these children to depend on digital media to find connections, regulate emotions, and cope with their illnesses. The study aims to determine the prevalence of electronic addiction among minors undergoing regular hemodialysis for end-stage kidney disease (ESKD) while simultaneously assessing their parental styles and psychological adaptation.
A total of 60 subjects were enrolled in the study and divided into two groups. Group A: Thirty patients undergoing regular hemodialysis for ESKD. Group B: Thirty healthy subjects of matching age and sex. All the recruited participants were subjected to semi-structured clinical interviews according to our institute’s sheets, Child Behavior Checklist (CBCL), Digital Addiction Scale for Children (DASC), Parent Authority Questionnaire (PAQ), and Psychological Adaptation Scale.

Results
Patients patients had a significantly higher prevalence of digital addiction compared to healthy subjects, with an average score of (87.00 ± 21.23) in dialysis patients against (53.00 ± 25.20) in normal participants, demonstrating significant differences (p< 0.001). There was a significant correlation between the duration of dialysis treatment and the prevalence of digital addiction (r = -0.415, p-value = 0.023). A significant inverse correlation was found between digital addiction and the emotional subtype of the psychological adaption rating (r= -0.375, p= 0.041).

Conclusion
The findings revealed that dialysis patients have a higher incidence of digital addiction. Prolonged dialysis duration was significantly associated with increased digital addiction among patients.