bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reinforcement of tendon attachment to bioactive porous titanium by BMP‐2‐induced ectopic bone formation
Authors: So Kazutaka , Takemoto Mitsuru , Fujibayashi Shunsuke , Neo Masashi , Kokubo Tadashi , Nakamura Takashi 
Published: November 2009

AbstractAchieving a firm attachment between a tendon and a metal remains a major challenge in orthopedic surgery. In this study, we developed a simple model for evaluating the strength of this attachment using bioactive porous titanium, and confirmed whether bone morphogenic protein‐2 (BMP‐2) can be a help in achieving a firm attachment by ectopic bone formation. Rectangular plate‐shaped implants were soak‐loaded with BMP‐2 (B+ group) and were implanted within the patellar tendon of a rabbit. Implants without BMP‐2 (B− group) were used as controls, and they were harvested at 4 and 8 weeks postoperation for mechanical tests and for histological and histomorphometric study. The pull‐out failure load of the B+ group was significantly higher than that of the B− group and new bone was more prevalent within the pores and around the implants in the B+ group than in the B− group. The model used in this study was feasible for evaluating the tendon–metal attachment, and a combination of bioactive porous titanium and BMP‐2 was found to attach firmly to the tendon. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Pectin‐coated titanium implants are well‐tolerated in vivo
Authors: Kokkonen Hanna , Niiranen Helena , Schols Henk A. , Morra Marco , Stenbäck Frej , Tuukkanen Juha 
Published: November 2009

AbstractMultiform coated titanium implants are widely used in orthopedic and dental surgery. In this study, we have investigated the reactivity of pectin‐coated titanium samples implanted under the latissimus dorsi—muscle fascia of rats. Samples were coated with two enzyme treated apple pectins; modified hairy regions (MHR‐A and MHR‐B) that differed in chemical structure. Aminated (AMI) and uncoated titanium (Ti) served as controls. The thicknesses of the peri‐implant fibrous tissue capsules formed 1 or 3 weeks after implantation were measured as indicative of possible inflammatory reactions toward the biomaterials. After 1 week, the MHR‐B implant was surrounded by a thicker fibrous capsule (42.9 μm) than any of the other sample types: MHR‐A (33.2 μm), AMI (32.5 μm), and Ti (32.3 μm), the last one being the only statistically significant difference. After 3 weeks, however, this difference disappeared; the capsule thicknesses around MHR‐B and Ti implants had decreased to the values found for AMI and MHR‐A. Additionally, the capsule formation represents merely a stromal rather than an inflammatory reaction, as indicated by the absence of activated macrophages or foreign body giant cells in the capsules. These results indicate for the first time the in vivo tolerability of covalently linked pectins, and suggest the feasibility of pectin‐coated bone and dental implants for clinical use. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
The effect of receptor protein tyrosine phosphatase kappa on the change of cell adhesion and proliferation induced by N‐acetylglucosaminyltransferase V
Authors: Wang Can , Li Zengxia , Yang Zhaohua , Zhao Hongbo , Yang Yong , Chen Kangli , Cai Xiumei , Wang Liying , Shi Yinghong , Qiu Shuangjian , Fan Jia , Zha Xiliang 
Published: November 2009

AbstractN‐acetylglucosaminyltransferase V (GnT‐V) has been reported to be positively associated with tumor progression, but its mechanism still remains unknown. In the present study, we found that GnT‐V overexpression not only changed the glycosylation of receptor protein tyrosine phosphatase kappa (RPTPκ) but also decreased its protein level. Moreover, GnT‐V overexpression decreased cell calcium‐independent adhesion and increased the tyrosine phosphorylation level of β‐catenin, in which RPTPκ played an important role. Since RPTPκ has an RXKR motif, which is a favored cleavage site for furin, we used furin inhibitor to further explore the effect of RPTPκ on the change of cell adhesion and β‐catenin signaling induced by GnT‐V. Our results showed that preventing RPTPκ cleavage rescued the above effects of GnT‐V, suggesting that furin cleavage could be one of the factors for RPTPκ to regulate cell adhesion and β‐catenin signaling in GnT‐V overexpression cell lines. In addition, the increased tyrosine phosphorylation level of β‐catenin was associated with the increased nuclear level of β‐catenin and downstream signaling molecules such as c‐myc and cyclin D1 that were associated with cell proliferation. Our results suggest that GnT‐V could decrease human hepatoma SMMC‐7721 cell adhesion and promote cell proliferation partially through RPTPκ. J. Cell. Biochem. 109: 113–123, 2010. © 2009 Wiley‐Liss, Inc.
Nuclear redistribution of TCERG1 is required for its ability to inhibit the transcriptional and anti‐proliferative activities of C/EBPα
Authors: Banman Shanna L. , McFie Pamela J. , Wilson Heather L. , Roesler William J. 
Published: November 2009

AbstractTranscription elongation regulator 1 (TCERG1) is an inhibitor of transcriptional elongation, and interacts with transcription and splicing factors, suggesting that it assists in coupling and coordinating these two processes. Recently we showed that TCERG1 possesses an additional activity, that being to repress the transactivation and anti‐proliferative activities of the transcription factor CCAAT/Enhancer Binding Protein α (C/EBPα). In the present study, we provide evidence that TCERG1 functions as an inhibitor of C/EBPα rather than a transcriptional co‐repressor. This conclusion was based on reporter gene experiments demonstrating that TCERG1 was able to reverse not only C/EBPα‐mediated transactivation of promoter activity, but also C/EBPα‐mediated transrepression of a promoter which is inhibited by C/EBPα. These observations, along with our previous findings that TCERG1 inhibits cellular proliferation conferred by C/EBPα, support the relabeling of TCERG1 as an inhibitor C/EBPα. Using mutants of TCERG1, we showed that the inhibitory activity lies in the amino terminal region. Because C/EBPα and TCERG1 have been shown to occupy different subnuclear compartments, we examined whether nuclear relocalization of either protein was involved in the inhibition of C/EBPα by TCERG1. Using confocal microscopy, we showed that TCERG1 localizes to nuclear speckles in the absence of C/EBPα. However, when co‐expressed with C/EBPα, TCERG1 localizes to pericentromeric sites where C/EBPα resides. Nuclear redistribution of TCERG1 is required for its inhibitory activity, since mutants that did not display nuclear relocalization also lacked C/EBPα‐inhibitory activity. We propose that TCERG1 inhibits C/EBPα activity by keeping it retained in inactive, pericentromeric heterochromatin. J. Cell. Biochem. 109: 140–151, 2010. © 2009 Wiley‐Liss, Inc.
Protein kinases A and C and phosphatidylinositol 3 kinase regulate glycogen synthase kinase‐3A serine 21 phosphorylation in boar spermatozoa
Authors: Bragado Maria J. , Aparicio Ines M. , Gil Maria C. , Garcia‐Marin Luis J. 
Published: November 2009

AbstractThe cAMP‐dependent protein kinase (PKA), protein kinase C (PKC) and phosphatidylinositol 3‐kinase (PI3K) pathways control most relevant functions in male germ cells including motility. Recently we demonstrated that phosphorylation state of glycogen synthase kinase‐3α (GSK3A) is also a key event in the control of boar spermatozoa motility. However, the upstream regulators of GSK3A serine phosphorylation (inhibition) in male germ cells remain largely unknown. This work investigates the involvement of PKA, PKC and PI3K pathways in GSK3A phosphorylation in boar spermatozoa. A capacitating medium (TCM) or the phosphodiesterase‐resistant cell permeable cAMP analogue 8Br‐cAMP cause a significant increase in Ser21 GSK3A phosphorylation associated with a simultaneous significant increase in boar spermatozoa motility. These effects are blocked after preincubation of spermatozoa with PKA inhibitor H89 or PKC inhibitor Ro‐32‐0432. The PI3K inhibitor LY294002 increases both spermatozoa motility parameters and the basal GSK3A phosphorylation, but does not affect either TCM‐ or 8Br‐cAMP‐stimulated GSK3A phosphorylation. PI3K inhibition effects are mediated by an increase in intracellular cAMP levels in boar spermatozoa and are suppressed by PKA inhibitor H89. In summary, we demonstrate that PKA, PKC and PI3K pathways crosstalk in porcine male germ cells to crucially regulate GSK3A phosphorylation which subsequently controls cell motility. In addition, our results suggest that PI3K is upstream of PKA which lies upstream of PKC in this regulatory cascade(s). Our findings contribute to elucidate the molecular mechanisms underlying the regulation of one of the most relevant male germ cell functions, motility. J. Cell. Biochem. 109: 65–73, 2010. © 2009 Wiley‐Liss, Inc.
Effects of sodium butyrate on the differentiation of pancreatic and hepatic progenitor cells from mouse embryonic stem cells
Authors: Ren Meng , Yan Li , Shang Chang‐Zhen , Cao Jun , Lu Li‐Hong , Min Jun , Cheng Hua 
Published: November 2009

AbstractRecently significant progress has been made in differentiating embryonic stem (ES) cells toward pancreatic cells. However, little is known about the generation and identification of pancreatic progenitor cells from ES cells. Here we explored the influence of sodium butyrate on pancreatic progenitor differentiation, and investigated the different effects of sodium butyrate on pancreatic and hepatic progenitor formation. Our results indicated that different concentration and exposure time of sodium butyrate led to different differentiating trends of ES cells. A relatively lower concentration of sodium butyrate with shorter exposure time induced more pancreatic progenitor cell formation. When stimulated by a higher concentration and longer exposure time of sodium butyrate, ES cells differentiated toward hepatic progenitor cells rather than pancreatic progenitor cells. These progenitor cells could further mature into pancreatic and hepatic cells with the supplement of exogenous inducing factors. The resulting pancreatic cells expressed specific markers such as insulin and C‐peptide, and were capable of insulin secretion in response to glucose stimulation. The differentiated hepatocytes were characterized by the expression of a number of liver‐associated genes and proteins, and had the capability of glycogen storage. Thus, the current study demonstrated that sodium butyrate played different roles in inducing ES cells toward pancreatic or hepatic progenitor cells. These progenitor cells could be further induced into mature pancreatic cells and hepatocytes. This finding may facilitate the understanding of pancreatic and hepatic cell differentiation from ES cells, and provide a potential source of transplantable cells for cell‐replacement therapies. J. Cell. Biochem. 109: 236–244, 2010. © 2009 Wiley‐Liss, Inc.
Heterochrony in the germ ring closure and tail bud formation in embryonic development of rainbow trout (Oncorhynchus mykiss)
Authors: Finch Emma , Cruz Carlos , Sloman Katherine A. , Kudoh Tetsuhiro 
Published: November 2009

AbstractDue to their large yolk size, salmonid embryos take a longer time for epiboly movements and germ ring closure compared with most other teleost species. Here we analyzed the germ ring closure, tail bud formation and development of the notochord and somites in rainbow trout using live embryo imaging and in situ hybridization with the rt‐ntl probe. Rt‐ntl is expressed in the germ ring (blastula, gastrula and somitogenesis stage), notochord, tail bud and somites (somitogenesis stage). When epiboly covers half the yolk, a tail bud‐like structure is formed and somitogenesis starts. By the time epiboly is completed, the yolk covered and the germ ring closed, the embryo has already reached the 20 somite stage. Therefore, the timing of germ ring closure and tail bud formation is reversed in trout embryos compared with zebrafish and other small model fish embryos (heterochrony). Based on this result, we re‐examined the definition of tail bud formation. J. Exp. Zool. (Mol. Dev. Evol.) 314B:187–195, 2010. © 2009 Wiley‐Liss, Inc.
Characterization of major degradation products of an adenosine A2A receptor antagonist under stressed conditions by LC‐MS and FT tandem MS analysis
Authors: Zhang Li‐Kang , Pramanik Birendra N. 
Published: November 2009

AbstractParkinson's disease (PD) is a very serious neurological disorder, and current methods of treatment fail to achieve long‐term control. SCH 420814 is a potent, selective and orally active adenosine A2A receptor antagonist discovered by Schering‐Plough. Stability testing provides evidence of the quality of a bulk drug when exposed to the influence of environmental factors. Understanding the drug degradation profiles is critical to the safety and potency assessment of the drug candidate for clinical trials. As a result, identification of degradation products has taken an important role in drug development process. In this study, a rapid and sensitive method was developed for the structural determination of the degradation products of SCH 420814 formed under different forced conditions. The study utilizes a combination of liquid chromatography–tandem‐mass spectrometry (LC‐MS/MS) and Fourier Transform (FT) MS techniques to obtain complementary information for structure elucidation of the unknowns. This combination approach has significant impact on degradation product identification. A total of ten degradation products of SCH 420814 were characterized using the developed method. Copyright © 2009 John Wiley & Sons, Ltd.
EPR Analysis of n‐Butyl Acrylate Radical Polymerization
Authors: Barth Johannes , Buback Michael , Hesse Pascal , Sergeeva Tatiana 
Published: November 2009

AbstractVia electron paramagnetic resonance (EPR) spectroscopy, concentrations of secondary propagating radicals (SPRs) and tertiary mid‐chain radicals (MCRs) in n‐butyl acrylate solution polymerization were measured. The EPR spectrum is dominated by the 4‐line spectrum of SPRs at −50 °C and by the 7‐line spectrum of MCRs at +70 °C. At intermediate temperatures, a third spectral component is seen, which is assigned to an MCR species with restricted rotational mobility. The MCR components are produced by 1,5‐hydrogen shift (backbiting) of SPRs. The measured ratio of MCRs to SPRs allows for estimating the rate coefficient k for monomer addition to a mid‐chain radical. For 70 °C, k is obtained to be 65.5 L · mol−1 · s−1.magnified image
Dextran‐based coating system for the immobilization of cell adhesion promoting molecules on titanium surfaces
Authors: Dubs M. , Weisser J. , Linke R. , Pfuch A. , Imhof D. , Schnabelrauch M. 
Published: November 2009

AbstractImmobilization of adhesive peptides interacting with cellular integrin receptors onto metallic implant surfaces represents a promising approach to improve osseointegration of implants into the surrounding tissue. In the present study, a functional dextran‐based coating system consisting of an amino titanate adhesion promoter with dendritic structure and a carboxymethyl dextran was established to bind an RGD‐containing adhesive peptide via a selective coupling methodology onto titanium surfaces. The three‐step reaction procedure was characterized by X‐ray photoelectron spectroscopy. In cell adhesion experiments it could be demonstrated that dextran coatings containing immobilized RGD promote attachment and spreading of fibroblast and pre‐osteoblastic cells compared to native as well as CMD‐coated titanium surfaces without RGD. The direct attachment of the RGD sequence to the metal surface via the amino titanate adhesion promoter did not increase pre‐osteoblastic cell spreading, whereas coupling of RGD to the polymeric carboxy­methyl dextran layer slightly enhanced spreading of the cells.
A sequential auction‐bargaining procurement model
Authors: Huh Woonghee Tim , Park Kun Soo 
Published: November 2009

AbstractOne of the most important decisions that a firm faces in managing its supply chain is a procurement decision: selecting suitable suppliers among many potential competing sellers and reducing the purchase cost. While both auctions and bargaining have been extensively studied in the literature, the research that combines auctions and bargaining is limited. In this article, we consider a combined auction‐bargaining model in a setting where a single buyer procures an indivisible good from one of many competing sellers. The procurement model that we analyze is a sequential model consisting of the auction phase followed by the bargaining phase. In the auction phase, the sellers submit bids, and the seller with the lowest bid is selected as the winning bidder. In the bargaining phase, the buyer audits the cost of the winning seller and then negotiates with him to determine the final price. For this auction‐bargaining model, we find a symmetric equilibrium bidding strategy for the sellers in a closed form, which is simple to understand and closely related to the classical results in the auction and bargaining literature. We also show that the auction‐bargaining model generates at least as much profit to the buyer as the standard auction or sequential bargaining model. © 2009 Wiley Periodicals, Inc. Naval Research Logistics, 2010
Method for numerical integration of rapidly oscillating functions in diffraction theory
Authors: Mikš Antonín , Novák Jiří , Novák Pavel 
Published: November 2009

AbstractThe quadrature of general, highly oscillatory integrals is a relatively complicated computational problem that occurs in a wide range of practical applications, e.g. in physics, chemistry, and image analysis. It is often necessary to use a high number of nodal points with classical quadrature formulas in order to achieve a required accuracy of numerical integration of rapidly oscillating functions. However, an increase in integration points leads to a larger computational time. Our work describes and analyses a method for numerical integration of rapidly oscillating functions, which enables to obtain the required accuracy with a smaller number of nodal points than classical integration rules and with a relatively simple integration formula. The proposed method is generally suitable for integration of rapidly oscillating functions in various applications. The method is demonstrated in examples of calculation of the diffraction integral in optics, where the integrand is often a rapidly oscillatory function. The results can be readily adapted to similar problems from other fields. Copyright © 2009 John Wiley & Sons, Ltd.
Controlled synthesis of responsive hydrogel nanostructures via microcontact printing and ATRP
Authors: Chirra Hariharasudhan D. , Biswal Dipti , Hilt J. Zach 
Published: November 2009

AbstractSurfaces that are spatially functionalized with intelligent hydrogels, especially at the micro‐ and nanoscale, are of high interest in the diagnostic and therapeutic fields. Conventional methods of the semiconductor industry have been successfully employed for the patterning of hydrogels for various applications, but methods for fabricating precise 3 D patterns of hydrogels at the micro‐ and nanoscale over material surfaces remain limited. Herein, microcontact printing (µCP) followed by atom transfer radical polymerization (ATRP) was applied as a platform to synthesize temperature responsive poly(N‐isopropylacrylamide) hydrogels with varied network structures (e.g. different molecular weight crosslinkers) over gold surfaces. The XY control of the hydrogels was achieved using µCP, and the Z (thickness) control was achieved using ATRP. The controlled growth and the responsive behavior of hydrogels to temperature stimuli were characterized using Fourier transform infrared (FTIR) spectroscopy and atomic force microscopy (AFM). The results demonstrate that this platform allows for the controlled growth of hydrogel nanostructures using the controlled ATRP mechanism. It is also shown that the molecular weight of the crosslinker affects the rate of hydrogel growth. These PNIPAAm‐based crosslinked hydrogel patterns were also demonstrated to have a temperature‐dependent swelling response. Using this technique, it is possible to synthesize responsive hydrogel patterns over various surfaces for potential applications in the biomedical field. Copyright © 2009 John Wiley & Sons, Ltd.
Plasma protein C is a useful clinical marker for hepatic veno‐occlusive disease (VOD) in stem cell transplantation
Authors: Iguchi Akihiro , Kobayashi Ryoji , Kaneda Makoto , Kobayashi Kunihiko 
Published: November 2009

AbstractBackgroundHepatic veno‐occlusive disease (VOD) is one of the most serious complications in stem cell transplantation (SCT). Although plasma protein C activity decreases in VOD after SCT, the timeframe of plasma protein C activity decreases during SCT is not known.ProcedureWe examined levels of plasma protein C serially during the course of SCT to determine the critical level and risk factors for VOD.ResultsOf 151 children who received SCT, 12 of them (7.9%) developed VOD. The mean minimum protein C activity in patients with VOD was significantly lower compared to that in patients without VOD (P < 0.0001). Receiver operating characteristic curve analysis revealed that the critical plasma protein C activity (cut‐off point) for VOD was identified to be 34.5% with high sensitivity (100%) and specificity (83.3%), and the reduction of plasma protein C below the cut‐off level (day +6.50 ± 2.43) was observed mostly prior to the onset of VOD (day +7.33 ± 2.64). The patients receiving melphalan in conditioning were found to be at high risk for VOD (P = 0.003). Among the melphalan containing regimens, melphalan + carboplatin + etoposide was a significant risk factor for depression of plasma protein C (P = 0.037).ConclusionPlasma protein C level was a useful parameter of VOD after SCT, and activity below 34.5% was critical for VOD. The use of melphalan in conditioning causes a high risk for VOD. Pediatr Blood Cancer 2010;54:437–443. © 2009 Wiley‐Liss, Inc.
Congenital rubella pneumonitis complicated by Pneumocystis jiroveci infection with positive long term respiratory outcome: A case report and literature review
Authors: Sanchez M.O. , Chang A.B. 
Published: November 2009

AbstractRubella remains to be a significant illness in the developing countries because of limited access to immunizations. In congenital rubella syndrome, lung involvement becomes evident within the few months of life, as a manifestation of the “late onset rubella syndrome.” The lungs and other organs become involved secondary to immunopathologic mechanisms and immunodeficiency predisposes affected patients to opportunistic pathogens. We report the clinical, respiratory and immunologic data of a young boy who developed rubella pneumonitis and concomitant infection with Pneumocystis jiroveci. Despite the complicated clinical course, the child survived. At follow‐up he has a normal pulmonary examination, mild hyperinflation only on his chest radiograph, normal immunology and normal respiratory reactance and resistance. Pediatr Pulmonol. 2009; 44:1235–1239. © 2009 Wiley‐Liss, Inc.