bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electroanalytical Detection of n‐Butylamine at a Nickel/Carbon Nanotube Composite
Authors: Panchompoo Janjira , Aldous Leigh , Xiao Lei , Compton Richard G. 
Published: March 2010

AbstractWe report the electroanalytical detection of n‐butylamine at a nickel/carbon nanotube (Ni/CNT) composite. Scanning Electron Microscopy (SEM) characterisation of the composite demonstrated that it consisted of bulk nickel particles ca. 2 μm in diameter entangled in CNT bundles. The spontaneous formation of Ni(OH)2 was optimised, and comparison with a 3 mm nickel electrode showed that ca. 4 μg of the Ni/CNT composite cast on a 3 mm GC electrode possessed bulk nickel characteristics while also having higher activity and higher sensitivity towards the electrochemical detection of n‐butylamine. However, the Ni/CNT composite showed no response to ammonia, in contrast to the macro‐nickel‐electrode.
Polyethylenimine Functionalized with Dopamine: Characterization and Electrocatalytic Properties
Authors: Rubianes María D. , Strumia Miriam C. 
Published: March 2010

AbstractWe report the chemical modification of polyethylenimine (PEI) by functionalization with dopamine (Do) using glutaraldehyde (Glu) as a linker (PEI‐Glu‐Do). The new polymer was characterized by FT‐IR spectroscopy, by derivative UV‐vis spectroscopy, and Cyclic Voltammetry. The PEI‐Glu‐Do demonstrated to have an important electrocatalytic activity allowing an important decrease in the overvoltages for the oxidation of NADH and a significant increase in its oxidation peaks currents. Different ratios of PEI:Do were assayed and the best response was obtained using PEI : Glu : Do 1 : 2 : 1. The new polymer represents an interesting and promising alternative for the electrochemical quantification of NADH and other analytes of clinical interest, as well as for the development of new electrochemical (bio)sensors.
Development of a Glucose Biosensor Using Advanced Electrode Modified by Nanohybrid Composing Chemically Modified Graphene and Ionic Liquid
Authors: Yang Min Ho , Choi Bong Gill , Park HoSeok , Hong Won Hi , Lee Sang Yup , Park Tae Jung 
Published: March 2010

AbstractNanohybrids of chemically modified graphene (CMG) and ionic liquid (IL) were prepared by sonication to modify the electrode. The modified CMG‐IL electrodes showed a higher current and smaller peak‐to‐peak potential separation than a bare electrode due to the promoted electron transfer rate. Furthermore, the glucose oxidase (GOx) immobilized on the modified electrode displayed direct electron transfer rate and symmetrical redox potentials with a linear relationship at different scan rates. The fabricated GOx/CMG‐IL electrodes were developed selective glucose biosensor with respect to a sensitivity of 0.64 μA mM−1, detection limit of 0.376 mM, and response time of <5 s.
Microelectrode Sensing of Adenosine/Adenosine‐5′‐triphosphate with Fast‐Scan Cyclic Voltammetry
Authors: Xu Yida , Venton B. Jill 
Published: March 2010

AbstractAdenosine and adenosine‐5′‐triphosphate (ATP) are important extracellular signaling molecules. Here, we studied adenosine and ATP using fast‐scan cyclic voltammetry at carbon‐fiber microelectrodes. Although ATP and adenosine have similar oxidation potentials, ATP oxidation current was highly dependent on buffer pH and divalent cation concentrations but adenosine current was not. Therefore, they can be distinguished by adding a divalent cation chelator or calibrating electrodes at different pH values. The enzymatic degradation of adenosine by adenosine deaminase was monitored in a mixture of adenosine and ATP in presence of EDTA (ethylenediaminetetraacetate). This sensing method is promising for enzyme kinetics or in vitro studies.
Abrasive Stripping Voltammetric Studies of Lignin and Lignin Model Compounds
Authors: Chen Aicheng , Rogers Emma I. , Compton Richard G. 
Published: March 2010

AbstractLignin is potentially a major renewable, nonfossil source of aromatic and cyclohexyl compounds. In this study, we have investigated the abrasive stripping voltammetry of lignin and four lignin model compounds in the room temperature ionic liquids (RTILs) [C4mim][NTf2], [N6,2,2,2][NTf2] and [C4mim][OTf] (where [C4mim]+=1‐butyl‐3‐methylimidazolium, [N6,2,2,2]+=n‐hexyltriethylammonium, [NTf2]−=bis(trifluoromethanesulfonyl)imide and [OTf]− =trifluoromethanesulfonate) on a gold macrodisk and in 0.1 M H2SO4 and 0.1 M NaOH on a boron‐doped diamond (BDD) macroelectrode, with the hope of using the voltammetry to fingerprint the functional groups within the lignin molecule. The use of RTILs on metal electrodes, or either acidic or basic media in combination with BDD electrodes allows solvent systems with wide electrochemical potential windows, useful for studying voltammetry which may be difficult to observe in systems where early breakdown of the solvent occurs.
Graphene Based Electrochemical Sensors and Biosensors: A Review
Authors: Shao Yuyan , Wang Jun , Wu Hong , Liu Jun , Aksay Ilhan A. , Lin Yuehe 
Published: March 2010

AbstractGraphene, emerging as a true 2‐dimensional material, has received increasing attention due to its unique physicochemical properties (high surface area, excellent conductivity, high mechanical strength, and ease of functionalization and mass production). This article selectively reviews recent advances in graphene‐based electrochemical sensors and biosensors. In particular, graphene for direct electrochemistry of enzyme, its electrocatalytic activity toward small biomolecules (hydrogen peroxide, NADH, dopamine, etc.), and graphene‐based enzyme biosensors have been summarized in more detail; Graphene‐based DNA sensing and environmental analysis have been discussed. Future perspectives in this rapidly developing field are also discussed.
Bioaccumulation kinetics of organic xenobiotic pollutants in the freshwater invertebrateGammarus pulexmodeled with prediction intervals
Authors: Ashauer Roman , Caravatti Ivo , Hintermeister Anita , Escher Beate I. 
Published: March 2010

AbstractUptake and elimination rate constants, bioaccumulation factors, and elimination times in the freshwater arthropod Gammarus pulex were measured for 14 organic micropollutants covering a wide range of hydrophobicity (imidacloprid, aldicarb, ethylacrylate, 4,6-dinitro-o-cresol, carbofuran, malathion, 4-nitrobenzyl-chloride, 2,4-dichloroaniline, Sea-Nine, 2,4-dichlorophenol, diazinon, 2,4,5-trichlorophenol, 1,2,3-trichlorobenzene, and hexachlorobenzene; all 14C-labeled). The toxicokinetic parameters were determined by least-square fitting of a one-compartment first-order toxicokinetic model, followed by Markov Chain Monte Carlo parameter estimation. The parameter estimation methods used here account for decreasing aqueous concentrations during the exposure phase or increasing aqueous concentrations during the elimination phase of bioaccumulation experiments. It is not necessary to keep exposure concentrations constant or zero during uptake and elimination, respectively. Neither is it required to achieve steady state during the exposure phase; hence, tests can be shorter. Prediction intervals, which take the between-parameter correlation into account, were calculated for bioaccumulation factors and simulations of internal concentrations under variable exposure. The lipid content of Gammarus pulex was 1.3% of wet weight, consisting of 25% phospholipids and 75% triglycerides. Size-dependent bioaccumulation was observed for eight compounds, although the magnitudes of the relationships were too small to be of practical relevance. Elimination times ranged from 0.45 to 20 d, and bioaccumulation factors ranged from 1.7 to 4,449 L/kg. The identified compounds with unexpectedly long elimination times should be given priority in future studies investigating the biotransformation of these compounds. Environ. Toxicol. Chem. 2010;29:1625–1636. © 2010 SETAC
Unsteady shrinking sheet with mass transfer in a rotating fluid
Authors: Ali F. M. , Nazar R. , Arifin N. M. , Pop I. 
Published: March 2010

AbstractIn this paper, the problem of unsteady flow induced by a shrinking sheet with mass transfer in a rotating fluid is studied. The transformed boundary layer equations are solved numerically by an implicit finite‐difference scheme known as the Keller‐box method. The influence of rotation, unsteadiness and mass suction parameters on the reduced skin friction coefficients f″(0) and g′(0), as well as the lateral velocity and velocity profiles are presented and discussed in detail. Copyright © 2010 John Wiley & Sons, Ltd.
Novel finite particle formulations based on projection methodologies
Authors: Asprone D. , Auricchio F. , Reali A. 
Published: March 2010

AbstractParticle methods are among the most studied meshless approaches, with applications ranging from solid mechanics to fluid‐dynamics and thermo‐dynamics. The objective of the present paper is to analyze the behavior of finite particle formulations based on projection methodologies, investigating in particular how these approaches behave in approximating 1D and 2D second‐order problems. Moreover, the issue of choosing suitable projection functions is discussed and 1D and 2D numerical tests, showing the second‐order accuracy of the methods under investigation, are performed. Copyright © 2010 John Wiley & Sons, Ltd.
A unified sub‐cell force‐based discretization for cell‐centered Lagrangian hydrodynamics on polygonal grids
Authors: Maire Pierre‐Henri 
Published: March 2010

AbstractWe develop a general formalism to derive cell‐centered Lagrangian scheme, wherein numerical fluxes are expressed in terms of sub‐cell force. The general form of the sub‐cell force is obtained by requiring the scheme to satisfy a semi‐discrete entropy inequality. Sub‐cell force and nodal velocity are computed consistently with cell volume variation by means of a node‐centered solver, which results from total energy conservation. Numerical results demonstrate the accuracy and the robustness of this scheme. Copyright © 2010 John Wiley & Sons, Ltd.
Pregabalin in outpatient detoxification of subjects with mild‐to‐moderate alcohol withdrawal syndrome
Authors: Di Nicola M. , Martinotti G. , Tedeschi D. , Frustaci A. , Mazza M. , Sarchiapone M. , Pozzi G. , Bria P. , Janiri L. 
Published: March 2010

AbstractObjectiveIn this open, prospective study we aimed to investigate the efficacy, medical safety and practicability of pregabalin in outpatient detoxification of alcohol‐dependent patients with mild‐to‐moderate alcohol withdrawal syndrome (AWS). Craving reduction, improvement of psychiatric symptoms and quality of life were the secondary endpoints.MethodsForty alcohol dependent patients (DSM‐IV) were detoxified receiving 200–450 mg of pregabalin. Withdrawal (Clinical Institute Withdrawal Assessment for Alcohol (CIWA‐Ar)) and craving (Visual Analogue Scale (VAS); Obsessive and Compulsive Drinking Scale (OCDS)) rating scales were applied; psychiatric symptoms and quality of life were evaluated using the Symptom Check List‐90 Revised (SCL‐90‐R) and the QL‐Index, respectively. Relapsed and abstinent patients in the post‐detoxification evaluation have been compared.ResultsAlcohol withdrawal symptoms and craving for alcohol resulted significantly reduced (p < 0.001) over time after pregabalin treatment. Pregabalin also resulted in a favourable improvement in psychiatric symptoms and quality of life (p < 0.001).ConclusionsTo our knowledge, this is the first open, prospective study, about the possible use of pregabalin as an outpatient detoxification agent. These preliminary data show its efficacy and safety in the management of patients with mild‐to‐moderate AWS. Copyright © 2010 John Wiley & Sons, Ltd.
Switching stable patients with schizophrenia from depot and oral antipsychotics to long‐acting injectable risperidone: efficacy, quality of life and functional outcome
Authors: Lloyd Keith , Latif Muhammud A. , Simpson Steve , Shrestha Keshar L. 
Published: March 2010

AbstractObjectiveThis international, non‐randomised study evaluated maintained efficacy and safety of Risperidone Long‐Acting Injectable (RLAI) compared to previous medications. To investigate the possible effect of differences in national health care systems across Europe, the UK subset efficacy, health‐related quality of life (HRQoL) and functioning data are reported here.MethodsPatients with schizophrenia or other psychotic disorders, symptomatically stable on antipsychotic medication, received intramuscular injections of RLAI 25 mg (to a maximum of 50 mg) every 2 weeks for 6 months.ResultsOf 182 UK patients recruited; 79% had schizophrenia (87% paranoid), 21% other psychotic disorders; 96% had been hospitalised at sometime. Improvement in mean Positive and Negative Syndrome Scale (PANSS) total score was significant at 1 month through to endpoint (p = 0.0001). There were significant improvements in PANSS subscales (p ≤ 0.001), PANSS‐Marder subscores (p ≤ 0.0391), and in Clinical Global Impression‐Severity (p = 0.0001) at endpoint. Patients reported improvements in: HRQoL (p < 0.05), Global Assessment of Functioning (p = 0.0001) and patient satisfaction (p = 0.0001) at endpoint. The proportion of patients rating RLAI treatment as ‘very good’ increased from 10% to 40%. 55% (22/40) of patients hospitalised at baseline were discharged by endpoint.ConclusionSustained, significant improvement in symptom control, HRQoL and functioning was seen in stable patients treated with RLAI over 6 months following a switch from previous antipsychotic medications. Copyright © 2010 John Wiley & Sons, Ltd.
Prolactin fluctuation over the course of a day during treatments with three atypical antipsychotics in schizophrenic patients
Authors: Yasui‐Furukori Norio , Furukori Hanako , Sugawara Norio , Tsuchimine Shoko , Fujii Akira , Inoue Yoshimasa , Kaneko Sunao 
Published: March 2010

AbstractHyperprolactinemia is a frequent consequence of treatment with some antipsychotic agents. Although prolactin secretion varies over the course of a day and during psychological circumstances, there is little information in the literature regarding the time dependence of the prolactin response to antipsychotics. We evaluated prolactin levels in schizophrenic patients receiving risperidone (3 mg twice daily), olanzapine (10 mg twice daily), or perospirone (16 mg twice daily) for at least 4 weeks. The subjects were compared to matched healthy controls. Plasma sample collection for quantification of drug and prolactin levels was conducted before and 2, 4, 6, 8, and 12 h after the morning dosing. Prolactin concentrations before dosing during risperidone treatment were significantly higher than during treatment with olanzapine and perospirone in females. The daily fluctuation of prolactin concentration after perospirone treatment was larger than that observed after risperidone and olanzapine treatments. Areas under the plasma concentration‐time curves was greatest in subjects treated with risperidone, followed by perospirone and finally by olanzapine. These findings suggest that daily fluctuations in prolactin concentration after perospirone treatment are larger than following treatment with risperidone and olanzapine. The plasma concentration of prolactin during perospirone treatment therefore depends on the time of sampling. Copyright © 2010 John Wiley & Sons, Ltd.
Varenicline does not increase serum BDNF levels in patients with nicotine dependence
Authors: Umene‐Nakano Wakako , Yoshimura Reiji , Yoshii Chiharu , Hoshuyama Tsutomu , Hayashi Kenji , Hori Hikaru , Katsuki Asuka , Ikenouchi‐Sugita Atsuko , Nakamura Jun 
Published: March 2010

AbstractVarenicline, α4β2 nicotinic acetylcholine receptor (nAChR) partial agonist, is a new class of medications for treating nicotine dependence. As an α4β2 nAChR partial agonist, varenicline serves to reduce nicotine withdrawal symptoms, while high‐affinity binding of the agonist mitigates the reinforcing effects of smoking. In the present study, we compared serum brain‐derived neurotrophic factor (BDNF) levels of nicotine dependence and nonsmokers, and we investigated changes in serum BDNF levels after 8 weeks of treatment with varenicline. Patients met the DSM‐IV criteria for nicotine dependence. Both the Fagerström test for nicotine dependence (FTND) and the Tobacco Dependence Screener (TDS) were used. Serum BDNF levels and breath carbon monoxide (CO) levels were measured before and 8 weeks after varenicline treatment. Fourteen of 16 subjects (87.5%) stopped smoking within 12 weeks of varenicline treatment. Thirteen healthy nonsmokers who never had previously smoked were randomly selected as a control group. Serum BDNF levels of patients before treatment (4.8 ± 3.8 ng/ml) were significantly lower than those in the control group (12.4 ± 6.13 ng/ml). Serum BDNF levels had not increased from baseline (4.8 ± 3.8 ng/ml) to 8 weeks after varenicline treatment (3.0 ± 1.1 ng/ml) of patients. These results suggest that smoking might decrease serum BDNF levels and that treatment with varenicline for 8 weeks, combined with 12 weeks of not smoking, does not increase serum BDNF levels in smokers. Copyright © 2010 John Wiley & Sons, Ltd.
“Lechward K, Glaser W, Falkner S. ABCA4 is ubiquitously expressed in mouse tissues and forms high molecular weight complexes. J Cell Biochem. 2010 Jan 20. [Epub ahead of print]”
Authors: Lechward Katarzyna A. , Glaser Walter , Falkner Susanne 
Published: March 2010

AbstractABC transporters are the focus of extensive research attempts due to their natural ability of selective transport of huge variety of substances into and out of the cells. They are being a potential target for pharmacologists and drug designers as well as basic scientists. We were interested to study the expression patterns of mouse proteins which belong to the “A” family of ABCs as well as to analyze their protein–protein interactions. The most exciting finding came with the studies of ABCA4, which mRNA was distributed in several mouse tissues, including eyes, brain, heart, lungs, liver, and testis, and the corresponding protein was present in brain, heart, eyes, and testis. Previously, ABCA4 was described as retina‐specific transporter, therefore, we extended our research to clarify where ABCA4 is expressed on RNA level, where its protein is expressed and what are its interacting proteins, in tissues different then retina. By several techniques which utilized the protein‐specific antibody we proved that ABCA4 is not a retina‐specific ABC transporter and that we purified it from brain and testis as well as from eyes and the heart. Analysis of the co‐purifying proteins by mass‐spectrometry had shown that apart from ABCA4, ABCA1, and ABCC3 were present in cross‐linked fraction. We also identified map kinase 12 and jade1S protein as putative ABCA4 interacting proteins. J. Cell. Biochem. © 2010 Wiley‐Liss, Inc.
Factor structure of the treatment outcome package for children
Authors: Kraus David R. , Boswell James F. , Wright Aidan G.C. , Castonguay Louis G. , Pincus Aaron L. 
Published: March 2010

AbstractThe Treatment Outcome Package (TOP) is a behavioral health assessment and outcome battery with modules that assess a wide array of behavioral health symptoms and functioning, demographics, case‐mix variables, and treatment satisfaction. The TOP was developed to follow the design specifications set forth by the Core Battery Conference (Horowitz, Lambert, & Strupp, 1997), but also to ensure the battery's applicability to naturalistic treatment. The TOP also includes a child version that addresses recommendations recently voiced by several experts in youth assessment and treatment. The purpose of this manuscript is to document the process involved in developing the shortened version of the Child TOP. With large samples of diverse patients, 103 items were reduced to 48, with 13 stable and clinically useful subscales. The final confirmatory factor analysis of 7,267 patients replicated the model with excellent results. Although some of these subscales were similar to factors derived from the adult version of the TOP, others were specific to childhood disorders. In addition, the analyses demonstrated that the TOP is not restricted to manifestations of distress and impairment, but also captures a unique factor of childhood strengths (i.e., resiliency). © 2010 Wiley Periodicals, Inc. J Clin Psychol 66:1–14, 2010.
Calcium ion propagation in cultured keratinocytes and other cells in skin in response to hydraulic pressure stimulation
Authors: Goto Makiko , Ikeyama Kazuyuki , Tsutsumi Moe , Denda Sumiko , Denda Mitsuhiro 
Published: March 2010

AbstractWe have previously suggested that a variety of environmental factors might be first sensed by epidermal keratinocytes, which represent the frontier of the body. To further examine this idea, in the present study, we examined the intracellular calcium responses of cultured keratinocytes to external hydraulic pressure. First, we compared the responses of undifferentiated and differentiated keratinocytes with those of fibroblasts, vascular endothelial cells (VEC), and lymphatic endothelial cells. Elevation of intracellular calcium was observed after application of pressure to keratinocytes, fibroblasts, and VEC. The calcium propagation extended over a larger area and continued for a longer period of time in differentiated keratinocytes, as compared with the other cells. The response of the keratinocytes was dramatically reduced when the cells were incubated in medium without calcium. Application of a non‐selective transient receptor potential (TRP) channel blocker also attenuated the calcium response. These results suggest that differentiated keratinocytes are sensitive to external pressure and that TRP might be involved in the mechanism of their response. J. Cell. Physiol. 224:229–233, 2010 © 2010 Wiley‐Liss, Inc.
Expression of prothymosin alpha in meiotic and post‐meiotic germ cells during the first wave of rat spermatogenesis
Authors: Ferrara Diana , Izzo Gaia , Pariante Paolo , Donizetti Aldo , d'Istria Michela , Aniello Francesco , Minucci Sergio 
Published: March 2010

AbstractProthymosin alpha (PTMA) is a highly acidic small polypeptide, that is, widely distributed and conserved among mammals. Its possible involvement in male gametogenesis has been mentioned but not clarified yet; in particular, it has been suggested that, in non‐mammalian vertebrates, it could play a role during GC meiosis and differentiation. In the present work we investigated the possible association between PTMA and meiotic and post‐meiotic phases of mammalian spermatogenesis. Three different time points during postnatal development of rat testis were analyzed, that is, 27 dpp (completed meiosis), 35 dpp (occurring spermiogenesis), and 60 dpp (first wave of spermatogenesis definitely ended). RT‐PCR and Western blot analyses showed that the expression levels of both Ptma mRNA and corresponding protein decrease in total extracts from 27 to 60 dpp. The in situ hybridization localized the transcript in interstitial Leydig cells, peritubular myoid cells and, inside the tubules, in germ cells from pachytene spermatocytes to newly formed haploid spermatids. The immunohistochemistry analysis localized the protein in the same cell types at 27 dpp, while at 35 and 60 dpp the haploid cells remain the only germ cells that still express it. In particular, PTMA specific localization in the heads of spermatids and epididymal spermatozoa, associated with the acrosome system, supports for the first time the hypothesis of a direct function in male germ cells. J. Cell. Physiol. 224: 362–368, 2010. © 2010 Wiley‐Liss, Inc.