bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hybrid Capture™ Method for Detection of Human Papillomavirus DNA in Clinical Specimens: A Tool for Clinical Management of Equivocal Pap Smears and for Population Screening
Authors: Lörincz Attila T. 
Published: August 2010

Abstract and Key WordsHigh‐ and intermediate‐risk types of human papillomavirus (HPV) in concert with cofactors are responsible for over 90% of cervical cancers world‐wide. While the Pap smear is a valuable cancer prevention tool, its subjective nature leaves it error prone. As a result, HPV DNA testing appears to be a needed adjunct to the Pap smear. Hybrid Capture is a simple DNA test with high specificity and sensitivity which appears useful for clinical management of equivocal Pap tests and shows promise for mass screening.
Detection of Herpes Simplex DNA in Semen and Menstrual Blood of Individuals Attending an Infertility Clinic*
Authors: Borai Nadia El , Inoue Masato , Lefèvre Christophe , Naumova Elena N. , Sato Bunzo , Yamamura Masaichi 
Published: August 2010

AbstractObjective: To determine a possible link between herpes simplex virus 1 (HSV) and infertility.Method: A specifically designed polymerase chain reaction with nested primers, was developed and used to test for HSV in 153 men and 20 women attending an infertility clinic.Results: HSV DNA was detected in 37 (24%) out of 153 semen samples and in 11 (55%) out of 20 menstrual blood samples. However, HSV DNA (0%) was not detected in the semen of 16 males with children.A significant association between the evidence for infertility and an HSV positive test was observed in men (Fisher's exact test, p = 0.024), and a stronger effect was found in females after failed in vitro fertilization (Fisher's exact test p = 0.0086).Conclusion: This is the first report of the detection of herpes simplex virus DNA in semen and menstrual blood.Encouraging preliminary results justify antiviral therapy in case of a positive test.
Pregnancy and Delivery in Patients with von Willebrand's Disease
Authors: Ito Masazumi , Yoshimura Kouichi , Toyoda Nagayasu , Wada Hideo 
Published: August 2010

AbstractObjective: To explore the obstetrical problems that are encountered in women with von Willebrand's disease (vWD).Methods: Case histories of 6 women whose pregnancies were complicated by vWD were reviewed retrospectively over an 11‐year period. The disease had been diagnosed before pregnancy in only 2 cases. The 6 women had a total of 10 deliveries, 3 induced abortions, and 1 spontaneous abortion.Results: Although all 6 women had Type‐I vWD, their laboratory data and bleeding symptoms varied. The 10 full‐term deliveries were all vaginal. Heat‐treated concentrated Factor‐VIII preparations were administered in 2 cases, in both of which abortions were induced during the 2nd trimester. Genital bleeding persisted in the second case, so that the administration of Factor VIII was repeated, and the patient underwent uterine curettage on the 12th day after abortion. Desmopressin was administered before the early induction of abortion in 1 case. The patient with persistent genital bleeding after spontaneous abortion received Factor VIII for 5 consecutive days.Conclusion: Bleeding problems occurred about 1 week after delivery or at the time of induced abortion in the 6 women with vWD. Thus, such patients require special care during pregnancy.
Prenatal Diagnosis and Management of Urethral Obstruction
Authors: Rani Reddi , Cameron Alan , Munro Fraser 
Published: August 2010

AbstractEighteen cases of bladder outlet obstruction were diagnosed at Queen Mother's Haspital, Glasgow, over a period of 6 years. Commonest cause was posterior urethral valve in 11 cases (61%). Oligohydramnios was present in 78% of cases with a mortality of 67% mainly due to pulmonary hypoplasia. The 6 surviving infants (33%) underwent early postnatal surgery. Two of these have chronic renal failure on follow‐up.
A Case of Uterine Choriocarcinoma with Spontaneous Rupture Twenty‐Three Years following the Antecedent Pregnancy
Authors: Okamoto Tomomitsu , Nomura Seiji , Nakanishi Toru , Yamada Satoru , Tomoda Yutaka 
Published: August 2010

AbstractA 53‐year‐old woman went into shock with a spontaneous uterine rupture due to choriocarcinoma that occurred 23 years after an elective abortion in the first trimester, and she underwent an emergency hysterectomy. After 4 courses of uneventful postoperative chemotherapy, she suffered from severe pneumonia. Fortunately, however, she recovered and has remained in complete remission at 2.5 years follow‐up. This is a case of uterine choriocarcinoma with a spontaneous rupture after the longest latent period yet reported.
Ultrasonographic Diagnosis of Fetal Cholelithiasis
Authors: Nishi Takenori 
Published: August 2010

AbstractA case of opacity in the fetal gallbladder was diagnosed by ultrasound. The lesion resolved spontaneously in the postnatal period. Careful follow‐up ultrasound examinations are necessary for fetal cholelithiasis in the pre‐ and postnatal periods, and conservative management is mandatory.
Factors Influencing an Ultrasound‐Estimated Bone Mass in Postmenopausal Women
Authors: Ino Yasushi , Mizuno Kimio , Suzuki Akihiko , Tamakoshi Akiko , Kikkawa Fumitaka , Tomoda Yutaka 
Published: August 2010

AbstractObjective: There are various methods for determining bone‐mineral density for diagnosing osteoporosis. The most accurate method among them is dual‐energy X‐ray absorptiometry (DEXA), and the simplest one utilizes an ultrasound bone densitometer. We investigated factors influencing the correlation between bone‐density values determined by dual‐energy X‐ray absorptiometry and by the use of an ultrasound bone densitometer.Methods: Sixty‐seven postmenopausal Japanese women aged from 31 to 68 years old were enrolled in the study. Bone‐mineral densities (BMDs) of the lumbar spine (L2–4) measured by DEXA and broadband ultrasound attenuation (BUA) of the right os calcaneus measured by an ultrasound bone densitometer were subjected to statistical analysis.Results: Multivariate analysis indicated that the patient's exercise history was useful — following the number of years since menopause and body weight — for determining lumbar spine bone‐mineral density. The influence of hormone replacement therapy (HRT) on bone mass in postmenopausal women is also considered to be marked, but the results of the present multivariate analysis showed almost no influence on BUA or the L2–4 BMD.Conclusions: The patient's exercise history should be taken into consideration when lumbar spine bone‐mineral density is estimated using an ultrasound bone densitometer instead of DEXA.
A Phenotypically Normal Liveborn Male after Prenatal Diagnosis of Trisomy 20 Mosaicism
Authors: Hayashi Satoshi , Miharu Norio , He Hu , Honda Hiroshi , Samura Osamu , Nakaoka Yoshiharu , Ohama Koso 
Published: August 2010

AbstractWe report on a case of prenatally diagnosed trisomy 20 mosaicism. Conventional cytogenetic analysis and fluorescence in situ hybridization (FISH) using a chromosome 20 specific probe were performed on the lymphocytes and extra‐embryonic tissues after birth. All of them revealed normal karyotypes. The baby is developing normally at the age of 2 years.
Evaluation of Patients with Active Pulmonary Tuberculosis for Genital Involvement
Authors: Aka Nurettin , Vural E. Zeynep Tuzcular 
Published: August 2010

AbstractFifty‐seven women hospitalized for active pulmonary tuberculosis were evaluated for genital involvement using menstrual blood culture, curettage and hystero‐salpingography. Seven patients (12.3%) were diagnosed as genital tuberculosis. Infertility was present in 71.4% of our patients.
Rapid Polymerase Chain Reaction Analysis of St14 (DXS52) VNTR: Carrier Detection of Hemophilia A
Authors: Yang Young Ho , Song Kyung Soon , Kim In Kyu , Cha Dong Hyun 
Published: August 2010

AbstractObjective: To determine the frequency of St14 VNTR allele in Koreans as a marker of the hemophilia A and to evaluate the efficacy of this marker for carrier detection of hemophilia AMethods: PCR amplified RFLP analysis of St14 VNTR was done in 312 X‐chrombsomes of 122 unrelated Korean males and 95 females and the same method was applied to carrier detection in the 2 hemophilia A families.Results: There were 13 alleles of different sizes of St14 VNTR locus appeared in 312 X‐chromosomes of unrelated Koreans.For carrier detection of hemophilia A, in the family A, the mother showed 1390/1330 bp alleles and the father showed 700 bp allele. The affected son has inherited 1390 bp allele from his mother. The daughter at risk showed 1330/700 bp alleles. In family B, the mother showed 1280/700 bp alleles and the stepfather showed 1390 bp allele. The affected son has inherited 1280 bp allele. The daughter at risk showed 1390/700 bp alleles. And so the daughters of the 2 families were not carriers for hemophilia A.Conclusion: PCR analysis of St14 VNTR was a useful tool for carrier detection of hemophilia A.
Secular Rates of Twinning in Asia: Recent Observations and Review of Literature*
Authors: Shek Yuhang , Huang Amy , Shek Yuanfu , Keith Louis 
Published: August 2010

AbstractObjective: To study the rates of twinning in Asian countries using the most recent data available.Method: A Medline search was performed and all primary and secondary references obtained.Results: Data was obtained from 5 Asian countries from reports published between 1983 and 1993. Total twinning rates varied.Conclusion: Twinning among Asians is much more variable than had been reported previously. Further documentation in this area is indicated.
A Study of Tubo‐Ovarian and Ovarian Abscesses, with a Focus on Cases with Endometrioma
Authors: Kubota Takeyoshi , Ishi Kazuhisa , Takeuchi Hisaya 
Published: August 2010

AbstractObjective: To determine the incidence and causes of endometrioma‐associated tubo‐ovarian abscesses (TOAs) and ovarian abscesses.Methods: The medical records of 6,557 gynecologic inpatients were reviewed, and the data were analyzed using the median test and χ2 test.Results: The incidence of TOAs was 2.3% (5/218) in patients with endometrioma, and 0.2% (11/6,339) in patients without endometrioma (p = 0.0001). Among the TOA cases (n = 16), no significant differences in age, parity, history of pelvic surgery, or isolated organisms were observed between the subgroups with (n = 5) and without endometrioma (n = 11). There were only 2 cases of ovarian abscess, and both were associated with endometrioma. The causes of the abscesses in the 7 cases with endometrioma were contamination during surgery (1 case), contamination during a transvaginal endometrioma aspiration (1 case), and ascending infection (1 case), and unknown in 4 cases.Conclusions: The presence of endometrioma is a risk factor for the development of a TOA or an ovarian abscess.
Dengue Hemorrhagic Fever during Pregnancy: Antepartum, Intrapartum and Postpartum Management
Authors: Bunyavejchevin Suvit , Tanawattanacharoen Somchai , Taechakraichana Nimit , Thisyakorn Usa , Tannirandorn Yuen , Limpaphayom Kobchitt 
Published: August 2010

AbstractDengue hemorrhagic fever is a common tropical disease in Thailand that nowadays has an increasing incidence during adulthood.We managed three cases of dengue hemorrhagic fever during pregnancy which developed during the antepartum, intrapartum and postpartum periods. We diagnosed dengue hemorrhagic fever during pregnancy with clinical pictures of fever, hemoconcentration and thrombocytopenia with serological proof in all cases. All cases were treated conservatively except for the second one, in which cesarean delivery was inevitable due to severe preeclampsia with unfavorable cervix. All patients and their babies were in good condition before discharge.With increasing incidence during adulthood, more cases of dengue hemorrhagic fever in pregnancy can be found. Conservative treatment should be conducted in all cases. We believe that this is the first case report of intrapartum dengue hemorrhagic fever during pregnancy.
The Immunotherapy during in vitro Fertilization and Embryo Transfer Cycles in Infertile Patients with Endometriosis
Authors: Kim Chung‐Hoon , Chae Hee‐Dong , Kang Byung‐Moon , Chang Yoon Seok , Mok Jung‐Eun 
Published: August 2010

AbstractObjective: To investigate if the immunotherapy with corticosteroids would improve the pregnancy rate in infertile patients with endometriosis who undergo in vitro fertilization and embryo transfer (IVF‐ET).Methods: Forty‐two infertile patients with endometriosis plus tubal factor and 87 pure tubal infertility patients who underwent IVF‐ET in our unit were allocated randomly to the corticosteroid treatment group and the control group.Results: The prevalence of autoantibodies (antinuclear antibody, lupus anticoagulant, anticardiolipin antibody, rheumatoid factor) was elevated significantly in patients with endometriosis plus tubal factor compared with pure tubal infertility patients (38.1% vs 2.3%). Twenty‐one patients with endometriosis plus tubal factor underwent 54 cycles of IVF‐ET, receiving corticosteroids. Forty‐three patients with pure tubal factor underwent 81 cycles of IVF‐ET, receiving corticosteroids. Twenty‐one patients with endometriosis plus tubal factor who underwent 57 cycles of IVF‐ET and 44 patients with pure tubal factor who underwent 84 cycles of IVF‐ET served as controls, not receiving corticosteroids. In patients with endometriosis plus tubal factor, there was a significantly higher clinical pregnancy rate per cycle in the treatment group, with 42.6% (23/54) compared with 22.8% (13/57) in the control group but no differences between 2 groups in spontaneous abortion rate (21.7% vs 15.4%) and multiple pregnancy rate (17.4% vs 15.4%). In patients with pure tubal infertility, there were no significant differences between the treatment group and control group in clinical pregnancy rate (40.7% vs 34.5%), spontaneous abortion rate (12.1% vs 10.3%) or multiple pregnancy rate (18.2% vs 10.3%). In the endometriosis plus tubal infertility group with autoantibodies, the clinical pregnancy rate per cycle was significantly higher in the treatment group at 40.9% compared with 14.8% in the control group. In endometriosis plus tubal infertility group without autoantibodies, there was no significant difference between 2 groups with respect to the clinical pregnancy rate per cycle (43.8% vs 30.0%).Conclusions: This study suggests that immunotherapy with corticosteroids could improve the clinical pregnancy rate in endometriosis patients undergoing IVF‐ET and may be more effective in patients with positive autoantibodies.
Integrin Adhesion Molecules in the Endometrial Glandular Epithelium in Patients with Endometriosis or Adenomyosis
Authors: Ota Hirotaka , Tanaka Toshinobu 
Published: August 2010

AbstractObjective: To assess the role of β1 ‐integrin and E‐cadherin molecules in the eutopic glandular epithelium in patients with endometriosis or adenomyosis.Study Design: Twenty‐four patients with endometriosis, and 22 patients with adenomyosis diagnosed histologically were selected as subjects. The controls consisted of 29 fertile women. Eutopic endometria were obtained by curettage or immediately after the operation. The samples were immunohistochemically examined for the expression of very late activation antigen‐2 (VLA‐2), VLA‐3, VLA‐4, VLA‐5, VLA‐6, and E‐cadherin.Results: The expression of each VLA molecule and E‐cadherin except VLA‐4, VLA‐5, and VLA‐6 was significantly increased throughout the menstrual cycle in endometria in both the endometriosis and adenomyosis groups. In contrast, the expression of VLA‐4 in the adenomyosis group was significantly reduced in the secretory phase.Conclusion: Altered expression of β1‐integrins and E‐cadherin was observed throughout the menstrual cycle in patients with endometriosis and adenomyosis, suggesting the defective microenvironment of the endometrium.
Cancer of the Cervix in Pregnancy: A 31‐Year Experience at the Philippine General Hospital
Authors: Manuel‐Limson Genara A. , Ladines‐Llave Cecilia A. , Sotto Luciano S. J. , Manalo Augusto M. 
Published: August 2010

AbstractObjective: To determine whether pregnancy, manner of delivery, the type and timing of therapy would affect the course of cervical cancer.Methods: Records of cervical cancer cases at the Philippine General Hospital from 1961 to 1990 were reviewed.Results: Survival rate (SR) for cancer of the cervix in pregnancy was not different from that of the non‐pregnant. Those who delivered abdominally and treated by surgery posted a higher SR compared to those delivered vaginally and treated by radiotherapy. Majority of those delivered abdominally or had surgery were in Stage I while majority of those delivered vaginally or had radiotherapy were in the late stages. Nodal metastasis was associated with poorer prognosis.Conclusions: Survival rates were not affected by pregnancy nor by the manner of delivery, the mode and timing of treatment, but were still largely determined by the stage and by the presence of nodal metastasis.
Maternal Perception of Sound Provoked Fetal Movement for Antepartum Assessment of Fetal Well‐being
Authors: Chittacharoen A. , Herabutya Y. , Tungsagonwattana S. , Suthutvoravut S. 
Published: August 2010

AbstractObjective: To assess the reliability and application of maternal perception of sound provoked fetal movement for antepartum assessment of fetal well‐being.Methods: Six hundred singleton high risk pregnant women of at least 32 weeks gestation were studied on 684 occasions. The response was compared with a nonstress test (NST) performed before a 3 second vibroacoustic stimulation with an electronic artificial larynx. The result of maternal perception of sound provoked fetal movement was compared to the NST and the perinatal outcome using sensitivity, specificity, negative predictive value, positive predictive value, and accuracy.Results: On 684 occasions, a positive response to sound stimulation, recorded as a fetal movement by the mother, occurred on 642 occasions (93.9%) and was accompanied by a reactive NST on 630 occasions; giving a specificity of 97.1% and a negative predictive value of 98.1%. Results of maternal perception of sound provoked fetal movement and NST, performed within a week of delivery, in 560 women were compared with perinatal outcome. The maternal perception of sound provoked fetal movement test had better sensitivity (64.7%) for poor perinatal outcome than the NST (58.8%). Both tests were not different in specificity (96.3% vs 96.5%), positive predictive value (35.5% vs 34.5%), negative predictive value (98.9% vs 98.7%), and accuracy (95.4% vs 95.4%).Conclusion: The findings suggested that maternal perception of sound provoked fetal movement may be an effective screening test to identify fetal compromise in antepartum period. This test is a simple and inexpensive test for evaluating fetal well‐being in primary health care setting.
Ovarian Pregnancy Diagnosed in the Third Trimester: A Case Report
Authors: Seki Hiroyuki , Kuromaki Ken‐ichi , Takeda Satoru , Kinoshita Katsuyuki 
Published: August 2010

AbstractWe observed a patient with a primary ovarian pregnancy complicated by severe preeclampsia and a presumed fetal anomaly. Diagnosis was made at cesarean section at 30 weeks of gestation. Etoposide, given intravenously at a dose of 100 mg daily for 5 days, prevented massive bleeding during the procedure that was subsequently performed to separate the placenta.
Decoupled leaf and stem economics in rain forest trees
Authors: Baraloto Christopher , Timothy Paine C. E. , Poorter Lourens , Beauchene Jacques , Bonal Damien , Domenach Anne‐Marie , Hérault Bruno , Patiño Sandra , Roggy Jean‐Christophe , Chave Jerome 
Published: August 2010

Ecology Letters (2010) 13: 1338–1347AbstractCross‐species analyses of plant functional traits have shed light on factors contributing to differences in performance and distribution, but to date most studies have focused on either leaves or stems. We extend these tissue‐specific analyses of functional strategy towards a whole‐plant approach by integrating data on functional traits for 13 448 leaves and wood tissues from 4672 trees representing 668 species of Neotropical trees. Strong correlations amongst traits previously defined as the leaf economics spectrum reflect a tradeoff between investments in productive leaves with rapid turnover vs. costly physical leaf structure with a long revenue stream. A second axis of variation, the ‘stem economics spectrum’, defines a similar tradeoff at the stem level: dense wood vs. high wood water content and thick bark. Most importantly, these two axes are orthogonal, suggesting that tradeoffs operate independently at the leaf and at the stem levels. By simplifying the multivariate ecological strategies of tropical trees into positions along these two spectra, our results provide a basis to improve global vegetation models predicting responses of tropical forests to global change.
Influence of caspases 8 and 9 gene promoter polymorphism on prostate cancer susceptibility and early development of hormone refractory prostate cancer
Authors: Kesarwani Pravin , Mandal Raju K. , Maheshwari Ruchir , Mittal Rama Devi 
Published: August 2010

What’s known on the subject? and What does the study add? Earlier, role of caspases in cancer initiation and progression was documented. However, our paper is the first to find association of caspase gene polymorphism in PCa progression (CASP 8 ‐652) and metastasis (CASP 9‐1263 G). Moreover, our paper shows that the gene polymorphisms in the promoter region of the gene can play a major role in development of hormone refectory prostate cancer (CASP 8‐652). Results from this pilot study may be revaluated, and may be applicable in clinical practice. The PCa subjects with presence of CASP 8 ‐652 (‐/‐) allele may not be good subjects for hormone therapy, therefore these patients can be given any other alternative therapy.OBJECTIVE

To investigate, by genotyping CASP8 (−652 6N del/ins) and CASP9 (−1263 A > G; −293 19N del/ins), whether inactivation of apoptosis by genetic polymorphism of caspases 8 and 9 play an integral role in the mechanism of cancer development.
To investigate the role of these polymorphisms in susceptibility to early development of hormone refractory prostate cancer.

PATIENTS AND METHODS

The study included 175 histologically confirmed cases of prostate cancer and 198 age and ethnicity matched healthy controls.
CASP9−1263 A > G polymorphism was genotyped using the polymerase chain reaction–restriction fragment length polymorphism method.
CASP9−293 del/ins and CASP8−652 del/ins polymorphisms were genotyped and the deletion pattern analysed by polyacrylamide gel electrophoresis.

RESULTS

Our results demonstrated that presence of CASP9−1263 G allele was associated with reduced risk for prostate cancer (odds ratio 0.6, 95%CI 0.39–0.92, P= 0.02). Other variant CASP9 was not associated with prostate cancer risk.
Coincidentally, the presence of CASP9−1263 G allele was associated with increased risk for progression of prostate cancer to bone metastasis (odds ratio −2.28, 95%CI 1.14–4.53, P= 0.02).
CASP8–652 (+/−) genotype was associated with increased hazard for early development of hormone refractory prostate cancer (hazard ratio 2.44, 95%CI 1.2–5.85, P= 0.045).

CONCLUSION

Our results support the hypothesis that variants of CASP9 may influence the susceptibility to prostate cancer and its progression to bone metastasis.
CASP8 polymorphism may influence the progression of prostate cancer disease to a hormone refractory state.