bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effective use of plerixafor as a real‐time rescue strategy for patients poorly mobilizing autologous CD34+ cells
Authors: Gopal Ajay K. , Karami Mehdi , Mayor JoAl , Macebeo Mylene , Linenberger Michael , Bensinger William I. , Holmberg Leona 
Published: February 2012

AbstractPlerixafor enhances CD34+ cell mobilization, however, its optimal use is unknown. We hypothesized that plerixafor could “rescue” patients in the midst of mobilization when factors indicated a poor CD34+ yield. Of 295 consecutive autologous peripheral blood mobilization attempts at our center, 39 (13%) used plerixafor as rescue strategy due to a CD34+ cell concentration <10/μl (median 5.95/μl, n = 30), low CD34+ cell yield from prior apheresis day (median 1.06 × 106 CD34+ cells/kg, n = 7), or other (n = 2). Patients received a median of one plerixafor dose (range: 1–4). Thirty‐four (87%) collected =2 × 10 6 CD34+ cells/kg and 26 (67%) collected =4 × 10 6 CD34+ cells/kg. Median collections for lymphoma (n = 24) and myeloma (n = 15) patients were 4.1 × 106 and 8.3 × 106 CD34/kg, respectively. A single dose of plerixafor was associated with an increase in the mean peripheral blood CD34+ concentration of 17.2 cells/μl (P < 0.001) and mean increased CD34+ cell yield following a single apheresis of 5.11 × 106/kg (P < 0.03). A real‐time rescue use of plerixafor is feasible and may allow targeted use of this agent. J. Clin. Apheresis, 2012. © 2012 Wiley Periodicals, Inc.
Current status of additive solutions for platelets
Authors: Alhumaidan Hiba , Sweeney Joseph 
Published: February 2012

AbstractThe storage of platelets in additive solution (PAS) had lagged behind red cell concentrates, especially in North America. The partial or complete removal of anticoagulated plasma and storage of platelet concentrates in AS presents many advantages. The PAS can be formulated to optimize aerobic metabolism or decrease platelet activation, thus abrogating the platelet storage lesion and potentially improving in vivo viability. Plasma removal has been shown to reduce allergic reactions and the plasma harvested could contribute to the available plasma pool for transfusion or fractionation. PAS coupled to pathogen reduction technology results in a platelet product of equivalent hemostatic efficacy to conventionally stored platelets. Given the above, the likely future direction of platelet storage will be in new generation designer PAS with an extended shelf life and a superior safety profile to plasma stored platelets. J. Clin. Apheresis, 2012. © 2012 Wiley Periodicals, Inc.
Effects of nonionic micellar aggregates on the electron transfer reaction between l‐glutamic acid and gold(III) complexes
Authors: Sen Pratik K. , Gani Nasimul , Midya Jayanta K. , Pal Biswajit 
Published: February 2012

AbstractThe effect of nonionic micelles of Triton X‐100 on the oxidative decarboxylation of l‐glutamic acid by chloroaurate(III) complexes has been investigated in acetate buffer medium. The reaction is first order with respect to Au(III), but a complex order with respect to glutamate. H+ ion has both accelerating and retarding effects in the pH range 3.72–4.80, whereas a Cl− ion has an inhibiting effect in the range 0.02–0.56 mol dm−3. Under the experimental conditions, AuCl−4 and AuCl3(OH)− are the predominant and effective oxidizing species, whereas the zwitterion (H2A) and mononegative anion (HA−) are the predominant reducing species of the amino acid. The reaction involves a one‐step two‐electron transfer process and passes through the intermediate formation of iminic cation. In the presence of surfactant, the reaction passes through a maximum and it appears to follow Berezin's model, where both the oxidant and the substrate are partitioned between the aqueous and the micellar phase and then react. The binding constants between the reactants and the surfactant have been evaluated at different temperatures. Compensation between substrate–water interaction and substrate–micelle interaction plays an important role in such redox reactions in the presence of a surfactant. © 2012 Wiley Periodicals, Inc. Int J Chem Kinet 44: 482–493, 2012
A functional variation in the hypocretin neuropeptide precursor gene may be associated with obstructive sleep apnea syndrome in Japan
Authors: Ahmed Wael A. , Tsutsumi Makiko , Nakata Seiichi , Mori Terumi , Nishimura Yoichi , Fujisawa Toshiyuki , Kato Ichiro , Nakashima Mayuki , Kurahashi Hiroki , Suzuki Kenji 
Published: February 2012

AbstractObjectives/Hypothesis:To evaluate the association of hypocretin neuropeptide precursor gene (HCRT) variations with obstructive sleep apnea syndrome (OSAS) in a cohort of Japanese patients and to further evaluate whether the significant HCRT variations have potential functional consequences on HCRT expression.Study Design:Case‐control genetic association study.Methods:We studied the genetic variations within the HCRT gene. The study population consisted of 100 OSAS patients and 100 control subjects. The HCRT gene was amplified by polymerase chain reaction in all study subjects followed by direct sequencing and analysis of sequencing data.Results:Two genetic variations within the HCRT intron, IVS1+16T>C (rs9902709) and IVS1−69G>C, were identified with significant differences between patients and controls (P < .05). A reporter gene assay using HeLa cells showed that the construct containing the C allele of the rs9902709 variation had significantly higher luciferase activity compared with the construct containing the T allele (P = .002). Furthermore, enzyme immunoassay revealed that subjects with T/C and C/C genotypes for rs9902709 had 1.4‐fold and 1.5‐fold increases in sera levels of orexin‐A, respectively.Conclusions:Our genetic association study, followed by functional and quantitative phenotyping assays, demonstrated a functional locus within the HCRT gene, which may act to increase HCRT expression and lead to a protective effect against the development of OSAS. Laryngoscope, 2012
Radiofrequency volumetric tissue reduction of the inferior turbinate in a sheep model
Authors: Kakarala Kiran , Faquin William C. , Cunningham Michael J. 
Published: February 2012

AbstractObjectives/Hypothesis:To validate the sheep model for endoscopic nasal surgery, and to utilize this model to compare the immediate and early postoperative histopathologic effects of four electrosurgical turbinate reduction techniques.Study Design:Pilot comparative study of electrosurgical devices for inferior turbinate reduction using a sheep model.Methods:Three radiofrequency devices (one monopolar and two bipolar) were compared to monopolar electrosurgery in a sheep model of inferior turbinate reduction. Procedures were performed according to device manufacturer guidelines using standard endoscopic instruments. Normative acoustic rhinometry data were obtained for the sheep model. Histopathologic analysis of turbinate specimens was performed at postoperative day 0 and 21.Results:Turbinate reduction was performed on seven sheep, one procedure on each side, yielding 14 turbinate specimens. Acoustic rhinometry was validated in the sheep model and demonstrated increased nasal volumes following decongestion and turbinate reduction. Submucosal destruction of glands and venous sinusoids and replacement with fibrosis were demonstrated as a common mechanism of action for all four electrosurgical devices. Epithelial disruption was seen with all devices on postoperative day 0. Squamous metaplasia and normal respiratory epithelial regeneration were documented variably between devices at postoperative day 21.Conclusions:The sheep model is useful for study of both the anatomic and histopathologic effects of endonasal procedures. Standard endoscopic instruments and acoustic rhinometry can be used in this model with reproducible results. In this pilot animal study, radiofrequency devices for inferior turbinate reduction demonstrated greater preservation of normal nasal mucosal respiratory epithelium when compared to monopolar electrosurgery. Laryngoscope, 2012
Topical corticosteroids applied with a squirt system are more effective than a nasal spray for steroid‐dependent olfactory impairment
Authors: Shu Chih‐Hung , Lee Po‐Lei , Shiao An‐Suey , Chen Kee‐Tak , Lan Ming‐Ying 
Published: February 2012

AbstractObjectives/Hypothesis:Oral corticosteroids may restore conductive olfactory dysfunction that has been defined as steroid‐dependent olfactory loss, but the effect may be temporary. This study was designed to evaluate whether applying topical corticosteroids with a squirt system was more effective than using a nasal spray to maintain olfactory improvement following oral corticosteroids.Study Design:Prospective randomized trial enrolling 32 patients.Methods:Patients were enrolled if they had suffered from olfactory dysfunction for more than 3 months, and if their composite scores of odor threshold, discrimination, and identification scores in Sniffin' Sticks olfactory tests increased by more than six points after 1 week of oral corticosteroid treatment. A total of 32 patients were enrolled and randomized into two groups. All patients were treated with topical corticosteroids for 2 months using either the spray or squirt system, respectively.Results:Both measured and self‐rated olfactory functions after 1 and 2 months of topical corticosteroid treatment were better in the squirt group than in the spray group. However, 2 months of topical corticosteroid treatment with the squirt system only partially maintained olfactory improvement.Conclusions:The application of topical corticosteroids with a squirt system was more effective than with a spray in maintaining olfactory improvement with oral corticosteroid treatment. Nevertheless, it only partially maintained the improvement so that topical corticosteroid treatment using a squirt system needs to be combined with intervals of short‐term oral corticosteroids to treat steroid‐dependent olfactory loss while avoiding the side effects of long‐term oral corticosteroid use. Laryngoscope, 2012
Can fractional lasers enhance transdermal absorption of topical lidocaine in an in vivo animal model?
Authors: Oni Georgette , Brown Spencer A. , Kenkel Jeffrey M. 
Published: February 2012

AbstractBackground and ObjectiveIt has been shown in vitro that pretreatment of skin with fractional lasers enhances transdermal delivery of drugs. The aim of this study is to demonstrate in vivo firstly that laser enhances transdermal drug absorption and secondly that this can be manipulated by altering laser settings.Study Design/Materials and MethodsFour pigs were used in the IACUC approved animal study. On day 0, 5 g of 4% topical lidocaine was applied under occlusion for 60 minutes to a 400 cm2 area on the abdomen. Blood was drawn at 0, 60, 90, 120, 180, and 240 minutes. On day 7, the Er:YAG laser was used at 500, 250, 50, and 25 µm ablative depth, respectively, over a 400 cm2 area on the abdomen. Five grams of 4% topical lidocaine was applied immediately with occlusion for 60 minutes, and then removed. Blood was drawn at 0, 60, 90, 120, 180, and 240 minutes. The serum was extracted and analyzed for lidocaine and its metabolite monoethylglycinexylidide (MEGX).ResultsSerum levels of lidocaine and MEGX were undetectable in untreated skin. Following laser treatment both lidocaine and MEGX were detectable. Peak levels of lidocaine were significantly higher (P = 0.0002) at 250 µm (0.62 mg/L), compared to 500 µm (0.45 mg/L), 50 µm (0.48 mg/L), and 25 µm (0.3 mg/L). Peak levels of MEGX were significantly higher (P ≤ 0.0001) at 250 µm (0.048 mg/L), compared to 500 µm (0.018 mg/L), 50 µm (0.036 mg/L), and 25 µm (0.0144 mg/L).ConclusionsThis study demonstrates that laser pretreatment significantly increases absorption of topical lidocaine so that it is detectable in the blood and that manipulating laser settings can affect drug absorption. Future work will look at translating this effect into clinical benefit. Lasers Surg. Med. 44:168–174, 2012. © 2012 Wiley Periodicals, Inc.
Immunohistochemistry of angiogenesis mediators before and after pulsed dye laser treatment of angiomas
Authors: Laquer Vivian T. , Dao Belinda M. , Pavlis Janelle M. , Nguyen Amy N. , Chen Tina S. , Harris Ronald M. , Rugg Elizabeth L. , Kelly Kristen M. 
Published: February 2012

AbstractBackground and ObjectiveTissue effects of vascular lesion laser treatment are incompletely understood. Injury caused by pulsed dye laser (PDL) treatment may result in altered expression of mediators associated with angiogenesis.Materials and MethodsEight human subjects had one angioma treated with PDL (7 mm, 1.5 millisecond pulse duration, 9 J/cm2, cryogen spray cooling of 30 millisecond with a 30 millisecond delay). One week later, three biopsies were taken: normal skin, untreated angioma, angioma post‐PDL. Tissue was frozen and sections processed for immunohistochemistry staining of vascular endothelial growth factor (VEGF), basic fibroblast growth factor (bFGF), matrix metalloproteinase 9 (MMP‐9), and angiopoietin 2 (ANG‐2). Images were graded in a blinded fashion by a board certified dermatopathologist.ResultsThere were no clear trends in VEGF expression in the epidermis, dermis, or endothelial cells. As compared to normal skin, angiomas demonstrated the following: bFGF was decreased in the epidermis; MMP‐9 was decreased or unchanged in the epidermis and increased in the endothelial cells; ANG‐2 was increased in the endothelial cells. When comparing normal skin to angiomas + PDL, bFGF was decreased in the epidermis and increased in the dermis; MMP‐9 was decreased or unchanged in the epidermis; ANG‐2 was again increased in the endothelial cells. Comparison of staining in angioma to angioma + PDL samples revealed increased dermal bFGF expression.ConclusionAlterations in angiogenesis mediators were noted after PDL. Angiogenesis mediator changes associated with PDL treatment differed from those previously reported for incisional biopsies. This pilot study can guide future work on laser‐induced alterations in vascular lesions and such information may ultimately be used to optimize treatment outcomes. Lasers Surg. Med. 44:205–210, 2012. © 2012 Wiley Periodicals, Inc.
“Ermutigung brauchten wir beide nicht”
Authors: Zbikowski Frauke 
Published: February 2012

AbstractMarek Checinski und Alexander Kulesza sind theoretische Chemiker. Aus ihrem Wissen über die Dichtefunktionaltheorie haben sie eine Dienstleistung gemacht und berechnen für Industriekunden die Eigenschaften von Katalysatoren und die Verläufe katalytischer Prozesse.
Vom energetischen Imperativ zur nachhaltigen Chemie
Authors: Reschetilowski Wladimir 
Published: February 2012

AbstractDass der Mensch durch seine produktive Tätigkeit maßlos Güter anhäuft und so Gefahr läuft, bei einer Übernutzung der natürlichen Ressourcen sich selbst seiner Lebensgrundlage zu entziehen, befürchtete schon Wilhelm Ostwald vor 100 Jahren. Seine Antwort darauf ist heute noch immer aktuell: “Vergeude keine Energie, verwerte sie!”
Analytik in der Industrie
Authors: Sippel Ann‐Kathrin 
Published: February 2012

AbstractDie erste Frühjahrsschule industrielle Analytik gab Studenten höherer Semester einen Einblick in die analytische Chemie, wie sie die chemische und pharmazeutische Industrie einsetzt. Den theoretischen Teil ergänzte ein Industriepraktikum.
Two methods for pore network of porous media
Authors: Gao Shengyan , Meegoda Jay N. , Hu Liming 
Published: February 2012

SUMMARYTwo approaches of generating pore networks of porous media are presented to capture the pore fabric. The first methodology extracted pore structure from a computer simulated packing of spheres. The modified Delaunay tessellation was used to describe the porous media, and modified Nelder–Mead method in conjunction with three pore‐merging algorithms was used to generate the pore size and coordination number distributions of the randomly packed spheres. The Biconical Abscissa Asymmetric CONcentric bond was used to describe the connection between two adjacent voids. This algorithm was validated by predicting pore structure of a cubic array of spheres of equal radius with known pore sizes, throat sizes and coordination number distributions. The predicted distributions of pore structure agreed well with the measured. Then, the algorithm was used to predict pore structure and permeability of randomly packed spherical particles, and predicted permeability values were compared with published experimental data. The results showed that the predicted permeability values were in good agreement with those measured, confirming the proposed algorithm can capture the main flow paths of packed beds. The second methodology generated an equivalent pore network of porous media, of which the centers of voids were located in a regular lattice with constant pore center distance. However, this network allowed for matching both main geometrical and topological characteristics of the porous media. A comparison of the two approaches suggested that the second approach can also be used as a predictive tool to quantitatively study the microscopic properties of flow through porous media. Copyright © 2012 John Wiley & Sons, Ltd.
SERK2v2: A new second‐order stabilized explicit Runge‐Kutta method for stiff problems
Authors: Kleefeld B. , Martín‐Vaquero J. 
Published: February 2012

AbstractTraditionally, explicit numerical algorithms have not been used with stiff ordinary differential equations (ODEs) due to their stability. Implicit schemes are usually very expensive when used to solve systems of ODEs with very large dimension. Stabilized Runge‐Kutta methods (also called Runge–Kutta–Chebyshev methods) were proposed to try to avoid these difficulties. The Runge–Kutta methods are explicit methods with extended stability domains, usually along the negative real axis. They can easily be applied to large problem classes with low memory demand, they do not require algebra routines or the solution of large and complicated systems of nonlinear equations, and they are especially suited for discretizations using the method of lines of two and three dimensional parabolic partial differential equations. In Martín‐Vaquero and Janssen [Comput Phys Commun 180 (2009), 1802–1810], we showed that previous codes based on stabilized Runge–Kutta algorithms have some difficulties in solving problems with very large eigenvalues and we derived a new code, SERK2, based on sixth‐order polynomials. Here, we develop a new method based on second‐order polynomials with up to 250 stages and good stability properties. These methods are efficient numerical integrators of very stiff ODEs. Numerical experiments with both smooth and nonsmooth data support the efficiency and accuracy of the new algorithms when compared to other well‐known second‐order methods such as RKC and ROCK2. © 2012 Wiley Periodicals, Inc. Numer Methods Partial Differential Eq 2013
The Impact of Federal Preemption of State Antipredatory Lending Laws on the Foreclosure Crisis
Authors: Ding Lei , Quercia Roberto G. , Reid Carolina K. , White Alan M. 
Published: February 2012

AbstractState antipredatory lending laws (APLs) are designed to protect borrowers against predatory lending that can increase the risk of default and deplete the home equity held by borrowers. Federal regulators instituted preemption that limited the scope and reach of state antipredatory lending regulations for certain lenders. Based on the variation in state laws and the variation in the regulatory environment among lenders, this paper identifies the effects of federal preemption of state APLs on the quality of mortgages originated by preempted lenders. The results provide evidence of a relatively higher increase in default risk among loans exempted from strong state antipredatory laws. These results are most robust among refinance mortgages with adjustable interest rates—a large and highly dynamic market in the period of analysis. The findings provide initial evidence that preemption of state mortgage lending regulations may result in an increase in mortgage default risk, thus limiting consumer protection in the residential mortgage market.
An osteosarcoma zebrafish model implicates Mmp‐19 and Ets‐1 as well as reduced host immune response in angiogenesis and migration
Authors: Mohseny Alexander B , Xiao Wei , Carvalho Ralph , Spaink Herman P , Hogendoorn Pancras CW , Cleton‐Jansen Anne‐Marie 
Published: February 2012

AbstractAbout 40% of osteosarcoma patients die of metastases. Novel strategies to improve treatment of metastatic patients require a better understanding of the processes involved, like angiogenesis, migration, and the immune response. However, the rarity of osteosarcoma and its heterogeneity make this neoplasm difficult to study. Recently we reported malignant transformation of mouse mesenchymal stem cells (MSCs) which formed osteosarcoma upon transplantation into mice. Here we studied these cells in zebrafish embryos and found that transformed MSCs induced angiogenesis and migrated through the bodies of the embryos, but this was never observed with non‐transformed normal MSCs (progenitors of the transformed MSCs). Whole genome expression analysis of both the cells and the host showed that angiogenesis and migration‐related genes matrix metalloproteinase 19 (Mmp‐19) and erythroblastosis virus E26 oncogene homologue 1 (Ets‐1) were overexpressed in transformed MSCs compared to normal MSCs. Investigating the host response, embryos injected with transformed MSCs showed decreased expression of immune response‐related genes, especially major histocompatibility complex class 1 (mhc1ze), as compared to embryos injected with normal MSCs. These findings contribute to the identification of genetic events involved in angiogenesis, migration, and host response providing targets as well as an appropriate model for high‐throughput drug screens. Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Variable response to propranolol treatment of kaposiform hemangioendothelioma, tufted angioma, and Kasabach–Merritt phenomenon
Authors: Chiu Yvonne E. , Drolet Beth A. , Blei Francine , Carcao Manuel , Fangusaro Jason , Kelly Michael E. , Krol Alfons , Lofgren Sabra , Mancini Anthony J. , Metry Denise W. , Recht Michael , Silverman Robert A. , Tom Wynnis L. , Pope Elena 
Published: February 2012

AbstractPropranolol is a non‐selective beta‐adrenergic antagonist successfully used in a case of kaposiform hemangioendothelioma (KHE) associated with Kasabach–Merritt phenomenon (KMP). We report 11 patients treated with propranolol for KHE and the related variant tufted angioma (TA), six of whom also had KMP. The varied responses to treatment, with only 36% responding in our series, demonstrate the need for further study of this medication before routine use for these indications. Pediatr Blood Cancer 2012; 59: 934–938. © 2012 Wiley Periodicals, Inc.
Retracted: Direct to consumer Internet advertising of statins: an assessment of safety
Authors: Williams Bethan , Brown David 
Published: February 2012

ABSTRACTThe following article from Pharmacoepidemiology and Drug Safety, “Direct to consumer Internet advertising of statins: an assessment of safety”, by Bethan Williams and David Brown, published online on 2nd February 2012 on Wiley Online Library (wileyonlinelibrary.com) and in Volume 21, Issue 4, pages 352–365, April 2012, has been retracted by agreement between the authors, the journal Editor‐in‐Chief, and John Wiley & Sons Ltd. The retraction has been agreed due to the inability to verify the accuracy of the data in Appendix 1.