bluuebunny/crossref_metadata_2025_split huggingface dataset:
Use of Adsorptive Transfer Stripping Voltammetry for Analyzing Variations of Cytosine Methylation in DNA
Authors: Ioannou Andrea , Alexiadou Despina , Kouidou Sofia , Girousi Stella , Voulgaropoulos Anastasios 
Published: November 2009

AbstractThe electrochemical behavior and thermal stability of double stranded oligonucleotides containing 5‐methyl‐cytosine and 7‐deaza‐guanosine as nucleotide analogues, as well as of Jurkat genomic DNAs methylated to different degree were studied by ACV and SWV and by thernal denaturation analysis. ACV and SWV combined with thermal denaturation analysis of the natural and modified oligonucleotides gave information regarding the presence of methylation and the concomitant conformational changes. ACV and SWV of Jurkat DNA mixtures methylated to different degrees revealed a decrease of the peak heights with increasing methylation, indicating an increase of structural rigidity, in agreement with the thermal denaturation data. These results verify the, possibly local, conformational changes introduced by DNA methylation. The results obtained in all cases were reproducible.
Fabrication of a New Electrochemiluminescent Sensing Interface Based on Zinc Oxide Nanorod
Authors: Xu Meizhu , Zhao Rongrong , Wang Jian , Chen Guonan 
Published: November 2009

AbstractA new electrochemiluminescence (ECL) sensing interface was established based on the zinc oxide nanorod in this paper. Firstly, the zinc oxide (ZnO) nanorod was prepared on an indium tin oxide (ITO) electrode surface by the method of constant current cathodic electrodeposition, on which the Nafion film was then modified, and finally ruthenium(II) tris(bipyridine) (Ru(bpy)32+) was immobilized at the ZnO nanorod/Nafion composite‐modified electrode. The sensing interface shows well ECL behaviors and perfect stability after being constant temperature treatment at 80 °C. The composite electrode was characterized by EIS, SEM and XRD. The results showed that the good stability maybe related to the water content of Nafion film.
Disposable Carbon Electrodes for Liquid Chromatographic Detection of Catecholamines in Blood Plasma Samples
Authors: Perati Pranathi R. , Cheng Jun , Jandik Petr , Hanko Valoran P. 
Published: November 2009

AbstractThis paper describes a comparative evaluation of disposable carbon electrodes and conventional glassy carbon electrodes. The detection of catecholamines was demonstrated in processed blood plasma and in the presence of catecholamine metabolites. Calibration plots of norepinephrine, epinephrine, 3,4‐dihydroxybenzylamine and dopamine were linear over three to four orders of magnitude with detection limits of 0.50, 0.73, 1.06, and 1.13 pg, respectively. The relative standard deviation of peak areas was ±2.3% for norepinephrine and ±5.3% for epinephrine from 30 injections of a 10 ng/mL mixed standard. Spike recoveries for norepinephrine, epinephrine, and dopamine from human blood plasma were 86±6%, 81±5%, and 77±4%, respectively.
Selective Electrochemical Determination of Paracetamol Using Nanostructured Film of Functionalized Thiadiazole Modified Electrode
Authors: Kalimuthu Palraj , Abraham John S. 
Published: November 2009

AbstractThis paper describes the selective electrochemical determination of paracetamol (PA) in the presence of important interferent, ascorbic acid (AA) using an ultrathin electropolymerized film of 5‐amino‐1,3,4‐thiadiazole‐2‐thiol (p‐ATT) modified glassy carbon (GC) electrode in 0.20 M phosphate buffer solution (pH 7.20). Bare GC electrode failed to resolve the voltammetric signals of AA and PA in a mixture. On the other hand, the p‐ATT modified electrode not only separated the voltammetric signals of AA and PA but also enhanced their peak currents. We achieved the lowest detection limit of 0.34 nM (S/N=3) for PA at p‐ATT modified electrode.
Electrocatalytic Oxidation of Folic Acid on Carbon Paste Electrode Modified by Nickel Ions Dispersed into Poly(o‐anisidine) Film
Authors: Ojani Reza , Raoof Jahan‐Bakhsh , Zamani Saeed 
Published: November 2009

AbstractPoly(o‐anisidine) (POA) was formed by successive cyclic voltammetry in monomer solution containing sodium dodecyl sulfate (SDS) at the surface of carbon paste electrode. Then Ni(II) ions were incorporated to electrode by immersion of the polymeric modified electrode having amine group in 0.1 M Ni(II) ion solution. Cyclic voltammetric and chronoamperometric experiments were used for the electrochemical study of this modified electrode; a good redox behavior of Ni(OH)2/NiOOH couple at the surface of electrode can be observed. The capability of this modified electrode for catalytic oxidation of folic acid was demonstrated. The amount of α and surface coverage (Γ*) of the redox species and catalytic chemical reaction rate constant (k) for folic acid oxidation were calculated. The catalytic oxidation peak current of folic acid was linearly dependent on its concentration and a linear calibration curve was obtained in the range of 0.1 to 5 mM with a correlation coefficient of 0.9994. The limit of detection (3σ) was determined as 0.091 mM. This electrocatalytic oxidation was used as simple, selective and precise voltammetric method for determination of folic acid in pharmaceutical preparations.
Effect of repeated laser microsurgical operations on laryngeal cancer prognosis
Authors: Karatzanis Alexander D. , Waldfahrer Frank , Psychogios Georgios , Hornung Joachim , Zenk Johannes , Velegrakis George A. , Iro Heinrich 
Published: November 2009

AbstractBackgroundThe aim of this study was to evaluate whether repeated sessions of transoral CO2 laser microsurgery (TLM) aiming to achieve clear histologic margins carry a negative effect on the prognosis of laryngeal cancer.MethodsThis was a retrospective evaluation of 763 cases that underwent primary TLM treatment for laryngeal cancer. Cases were compared for overall survival and local control rates with respect to status of surgical margins and number of procedures necessary to achieve these margins.ResultsNo significant differences were noted among cases with negative surgical margins regardless of the number of procedures necessary to achieve these margins. On the other hand, positive margins at the end of surgical treatment carried significant negative prognostic effect.ConclusionThe prognostic effect of negative surgical margins in TLM for laryngeal cancer is significant regardless of the number of procedures required to obtain such margins. © 2009 Wiley Periodicals, Inc. Head Neck, 2010
Estrogen‐induced angiogenic factors derived from stromal and cancer cells are differently regulated by enterolactone and genistein in human breast cancer in vivo
Authors: Saarinen Niina M. , Abrahamsson Annelie , Dabrosin Charlotta 
Published: November 2009

AbstractAngiogenesis is a key in cancer progression and its regulators are released both by the tumor cells and the stroma. Dietary phytoestrogens, such as the lignan enterolactone (ENL) and the isoflavone genistein (GEN), may differently affect breast cancer growth. In this study, human breast cancer cells (MCF‐7) were established in mice creating a tumor with species‐specific cancer and stroma cells. Ovariectomized athymic mice supplemented with estradiol (E2) were fed basal AIN‐93G diet (BD) or BD supplemented with 100 mg/kg ENL, 100 mg/kg GEN or their combination (ENL+GEN). We show that ENL and ENL+GEN inhibited E2‐induced cancer growth and angiogenesis, whereas GEN alone did not. Microdialysis was used to sample extracellular proteins in tumors in vivo. ENL and ENL+GEN decreased both stroma‐ and cancer cell‐derived VEGF, whereas cancer cell‐derived PlGF increased. In subcutaneous Matrigel plugs in mice, ENL and ENL+GEN decreased E2‐induced endothelial cell infiltration, whereas GEN alone did not. In endothelial cells, ENL inhibited E2‐induced VEGFR‐2 expression, whereas GEN did not. These results suggest that ENL has potent effects on breast cancer growth, even in combination with GEN, by downregulating E2‐stimulated angiogenic factors derived both from the stroma and the cancer cells, whereas dietary GEN does not possess any antiestrogenic effects.
Changes and tracking of mammographic density in relation to Pike's model of breast tissue aging: a UK longitudinal study
Authors: McCormack Valerie A. , Perry Nicholas M. , Vinnicombe Sarah J. , dos Santos Silva Isabel 
Published: November 2009

AbstractPercent mammographic density (PMD) is a strong marker of breast cancer risk. It may be a correlate of the rate of breast tissue aging, as proposed by Pike to explain breast cancer age‐incidence. We examined longitudinal changes in PMD in 645 breast screening attendees in London, UK, in which each had between 2 and 5 screens spanning 3–12 years at ages 50–65 years and compare these to Pike's model. Within‐woman PMD declined during these ages, with a slowing rate of decline. Annual rates of decline were 1.4% (95% confidence interval: 1.2–1.6), 0.7% (0.6–0.9) and 0.1% (–0.2 to 0.4) at ages 50, 57 and 64. Dense area declined similarly, but the absolute magnitude of the rate of increase of nondense area was almost double that of dense area. PMD dropped by 2.4% (1.4–3.4) on menopausal transition and increased by 2.4% (1.4–3.5) with the use of hormone therapy. Higher body mass index, greater parity and being Afro‐Caribbean or South Asian ethnicities were associated with lower PMD, but did not affect rate of change of PMD at these ages. Within‐woman rank correlation of PMD was 0.80 for readings taken 9 years apart. Effects of menopause and parity and the lack of effect of menarche on age‐specific PMD at these ages are consistent with the predicted determinants in Pike's model. A high degree of tracking of PMD indicates that at ages 50–65 years high‐risk women could be identified by a single early screen at age older than 50.
C3, C5, and factor B bind to chitosan without complement activation
Authors: Marchand C. , Bachand J. , Périnêt J. , Baraghis E. , Lamarre M. , Rivard G. E. , De Crescenzo G. , Hoemann C. D. 
Published: November 2009

AbstractChitosan is a polycationic and biocompatible polysaccharide composed of glucosamine and N‐acetyl glucosamine that is chemotactic for neutrophils and stimulates wound repair through mechanisms that remain unclear. It was previously shown that chitosan depletes complement proteins from plasma, suggesting that chitosan activates complement. Complement activation leads to cleavage of C5 to produce C5a, a neutrophil chemotactic factor. Here, we tested the hypothesis that chitosan generates C5a in human whole blood, citrated plasma, and serum. C5a fragment appeared in coagulating whole blood, and mixtures of chitosan‐glycerol phosphate/whole blood, in parallel with platelet and thrombin activation. However, in plasma and serum, thrombin and chitosan‐GP failed to generate C5a, although native C3, C5, and factor B adsorbed noncovalently to insoluble chitosan particles incubated in citrated plasma, serum, EDTA‐serum and methylamine‐treated plasma. By surface plasmon resonance, pure C3 adsorbed to chitosan. The profile of serum factors associating with chitosan was consistent with a model in which anionic blood proteins with a pI lower than the pK0 6.78 of chitosan (the upper limit of chitosan pKa) associate electrostatically with cationic chitosan particles. Zymosan, a yeast ghost particle, activated complement in serum and citrated plasma, but not in EDTA‐serum or methylamine plasma, to generate fluid‐phase C5a, while C3b formed covalent cross‐links with zymosan‐associated proteins and became rapidly cleaved to iC3b, with factor Bb stably associated. These data demonstrate that chitosan is a nonreactive biomaterial that does not directly activate complement, and provide a novel basis for predicting anionic serum protein–chitosan interactions. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Interaction of endothelial cells with methacrylic terpolymer biomaterials
Authors: Heath Daniel E. , Cooper Stuart L. 
Published: November 2009

AbstractA biomaterial system with tunable mechanical properties was synthesized through random copolymerization of hexyl methacrylate, methyl methacrylate, and methacrylic acid. The composition, molecular weight, water absorption, glass transition temperature, and tensile properties of the polymer system were characterized, and the biocompatibility of the material was demonstrated by the ability of human umbilical vein endothelial cells to adhere, proliferate, and spread on the polymer surface. No difference was observed in the behavior of the endothelial cells on the materials regardless of material stiffness. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
In vitro biological response to a self‐adhesive resin cement under different curing strategies
Authors: Monteiro Gabriela Q. M. , Souza Fábio B. , Pedrosa Ricardo F. , Sales Germana C. F. , Castro Célia Maria M. B. , Fraga Simone N. , Galvão Bruno H. A. , Braz Rodivan 
Published: November 2009

AbstractSelf‐adhesive resin cements represents a new approach in indirect restorative dentistry, although little is known about its biological properties. The influence of the curing strategy on the biological response of a self‐adhesive resin cement (Rely‐X Unicem/3M ESPE) was evaluated through methyltetrazolium (MTT) and nitric oxide (NOx) assays. Cylindrical specimens (n = 48) were prepared and cured according to the following strategies: photoactivation with halogen light‐curing unit (LCU) Demetron/Kerr, LED LCU Radii/SDI, and chemical activation. A control group with culture medium and a group with lipopolysaccharide, employed for cells stimulation, were used for comparisons (n = 8). After 24 h of direct contact between specimens and rat alveolar macrophages, the biological response was evaluated. Statistical evaluation of the data was performed with Kruskal‐Wallis and Dunn's test (p = 0.05). The MTT test showed that the specimens cured by halogen light and chemical activation provided higher alterations on cell metabolism. For LED‐cured specimens, cell viability was compatible to the results observed in the control group. For the NOx assay, all curing methods were cytotoxic to rat macrophages. Rely‐X Unicem demonstrated different cytotoxic effects according to the curing strategy employed. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Assessing peri‐implant tissue infection prevention in a percutaneous model
Authors: Perry Emily L. , Beck J. Peter , Williams Dustin L. , Bloebaum Roy D. 
Published: November 2009

AbstractBackground: Infection remains the main challenge to percutaneous, intramedullary osseointegrated implant technology. The purpose of this investigation was to determine if a broad spectrum antimicrobial, Ceragenin™ (CSA‐13) could prevent pin track infections in a percutaneous tibial pin site in a sheep model. Methods: In 20 sheep, a smooth titanium alloy pin/implant was inserted percutaneously through the medial skin and both cortices of the proximal tibia. In 10 sheep, the pin/skin interface was treated with a CSA‐13‐embedded foam pad. Ten sheep served as controls receiving an untreated pad. At the end of 24 weeks, or if they presented with clinical signs of infection, the animals were euthanized. Histological stains were processed from soft tissue and bone, and bacterial cultures were taken from tissue, bone, and blood. In addition to clinical signs, sheep were considered infected if at least one tissue culture and/or histologically stained sample was positive. Results: Compared with the controls, CSA‐13 did not prevent pin track infection (p = 0.88). Large gaps around the pin indicated a lack of skin‐pin adhesion. Conclusions: In this application, CSA‐13 was not effective in preventing pin track infections. This study suggests that maintaining skin attachment, at the implant surface of osseointegrated implants, is essential as a primary barrier to infection. Local antimicrobial treatments should be considered a secondary barrier to bacterial invasion of the pin/skin interface and deeper tissues. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Reduction in postlaminectomy epidural adhesions in sheep using a fibrin sealant‐based medicated adhesion barrier
Authors: Richards Peter J. , Turner A. Simon , Gisler Serge M. , Kraft Susan , Nuss Katja , Mark Silke , Seim Howard B. , Schense Jason 
Published: November 2009

AbstractEpidural adhesion formation is believed to be a central governing factor in the prevalence of pain after spinal surgery and is regarded as being the primary instigator of neural tethering, leading to complications during revision surgery. In this study, we assess the effectiveness and safety of fibrin sealant supplemented with tributyrin, termed Medicated Adhesion Barrier (MAB), as an alternative means of reducing the incidence of posterior spinal epidural adhesion formation. Laminectomy defects in sheep were treated with MAB, fibrin sealant alone, ADCON®Gel, or remained untreated. At 12 weeks postoperatively, the extent of fibrosis and epidural adhesion formation was evaluated using magnetic resonance imaging (MRI), peel‐off testing, and histological examination. Initial invitro analysis revealed that tributyrin was retained in fibrin gel in a time‐dependent manner and was an effective inhibitor of fibroblast proliferation. Treatment of sheep with MAB significantly reduced both the prevalence (p < 0.05) and tenacity (p < 0.05) of epidural adhesions. The effectiveness of MAB in preventing epidural adhesions was found to be comparable with that of ADCON®Gel. No adverse events were reported after the use of MAB. The MAB preparation seems to be an effective resorbable barrier for the prevention of epidural adhesions. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Effects of a 36% tooth bleaching gel on zinc phosphate cement
Authors: Boston Daniel W. , Jefferies Steven R. 
Published: November 2009

AbstractPurpose: To assess the effect of a 36% hydrogen peroxide bleaching gel on the roughness, microhardness, indentation modulus, and micromorphology of zinc phosphate cement aged 24 h or 1 week, compared to cement not exposed to bleaching gel. Methods: Dynamic indentation was performed for microhardness and indentation modulus. Contact profilometry was performed for surface roughness. Scanning electron microscopy assessed micromorphology. Means for microhardness, indentation modulus, and roughness were analyzed for differences among groups (No. 1 = 24 h/control; No. 2 = 24 h/treatment; No. 3 = 1 week/control; No. 4 = 1 week/treatment). Results: Means (SD) for groups 1–4 respectively: Vickers Microhardness (HV): 34.2(8.7)a, 32.8(10.9)a, 24.8(5.0)b, 41.5(7.4)c; Indentation Modulus (EIT, GPa): 14.2(6.9)a,b, 10.6(3.6)a, 11.2(3.8)a, 14.7(2.8)b; and Surface Roughness (Ra, microns): 1.08(0.38)a, 1.21(0.55)a, 1.55(0.21)b, 1.52(0.29)b. Means labeled with the same superscript were not statistically significantly different. Scanning electron microscopy of groups 1 and 2 revealed similar details. Group 3 surfaces had plate‐like crystals not present on the group 4 surfaces. Conclusions: Bleaching gel exposure increased hardness and indentation modulus and caused limited changes in surface morphology, but the cement was not adversely affected by the bleaching protocol. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Different inflammatory responses induced by three LDL‐lowering apheresis columns
Authors: Hovland Anders , Hardersen Randolf , Sexton Joe , Mollnes Tom Eirik , Lappegård Knut Tore 
Published: November 2009

AbstractLow‐density lipoprotein (LDL) apheresis is well‐established in selected patients with uncontrolled LDL levels. As such treatment affects biomarkers important in atherosclerosis and acute coronary syndromes, we systematically compared the inflammatory response induced by three LDL apheresis columns. Three patients with heterozygous familial hypercholesterolemia participated in a cross‐over study with six consecutive treatments with three different LDL apheresis columns: DL‐75 (whole blood adsorption), LA‐15 (plasma adsorption), and EC‐50W (plasma filtration). Biochemical parameters and inflammatory biomarkers, including complement activation products and 27 cytokines, chemokines, and growth factors were measured before and after treatment. Complement was activated through the alternative pathway. The final end product sC5b‐9 increased significantly (P < 0.01) and equally with all devices, whereas the anaphylatoxins C3a and C5a were lower by use of the adsorption columns. Hs‐CRP was reduced by 77% (DL‐75), 72% (LA‐15), and 43% (EC‐50W). The cytokines were consistently either increased (IL‐1ra, IP‐10, MCP‐1), decreased (IFN‐γ, TNF‐α, RANTES, PDGF, VEGF), or hardly changed (including IL‐6, IL8, MIP‐1αβ) during treatment. The changes were in general less pronounced with the adsorption columns. All columns reduced LDL significantly and to the same extent. In conclusion, three LDL‐apheresis devices with equal cholesterol‐lowering effect differed significantly with respect to the inflammatory response. J. Clin. Apheresis, 2009. © 2009 Wiley‐Liss, Inc.
Direct cell–cell contact between periodontal ligament fibroblasts and osteoclast precursors synergistically increases the expression of genes related to osteoclastogenesis
Authors: Bloemen Veerle , Schoenmaker Ton , de Vries Teun J. , Everts Vincent 
Published: November 2009

AbstractThe formation of bone resorbing osteoclasts in vivo is orchestrated by cells of the osteoblast lineage such as periodontal ligament fibroblasts that provide the proper signals to osteoclast precursors. Although the requirement of cell–cell interactions is widely acknowledged, it is unknown whether these interactions influence the expression of genes required for osteoclastogenesis and the ultimate formation of osteoclasts. In the present study we investigated the effect of cell–cell interaction on the mRNA expression of adhesion molecules and molecules involved in osteoclast formation in cultures of peripheral blood mononuclear cells (PBMCs) and human primary periodontal ligament fibroblasts, both as solitary cultures and in co‐culture. We further analyzed the formation of multinucleated, tartrate resistant acid phosphatase (TRACP) positive cells and assessed their bone resorbing abilities. Interestingly, gene expression of intercellular adhesion molecule‐1 (ICAM‐1) and of osteoclastogenesis‐related genes (RANKL, RANK, TNF‐α, and IL‐1β) was highly up‐regulated in the co‐cultures compared to mono‐cultures and the 5–10‐fold up‐regulation reflected a synergistic increase due to direct cell–cell interaction. This induction strongly overpowered the effects of known osteoclastogenesis inducers 1,25(OH)2VitD3 and dexamethasone. In case of indirect cell–cell contact mRNA expression was not altered, indicating that heterotypic adhesion is required for the increase in gene expression. In addition, the number of osteoclast‐like cells that were formed in co‐culture with periodontal ligament fibroblasts was significantly augmented compared to mono‐cultures. Our data indicate that cell–cell adhesion between osteoclast precursors and periodontal ligament fibroblasts significantly modulates the cellular response which favors the expression of osteoclast differentiation genes and the ultimate formation of osteoclasts. J. Cell. Physiol. 222: 565–573, 2010. © 2009 Wiley‐Liss, Inc.