bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endoscopic ultrasound‐guided paracentesis of ascitic fluid
Authors: Wardeh Rooba , Lee John G. , Gu Mai 
Published: November 2010

AbstractBACKGROUND:Endoscopic ultrasound (EUS)‐guided fine needle aspiration (FNA) has been widely used for the diagnosis of primary and metastatic gastrointestinal (GI) and non‐GI malignancies. Few studies have been published to evaluate the accuracy and the cytologic features of EUS‐guided paracentesis in the diagnosis and staging of malignant neoplasms.METHODS:All EUS‐guided paracenteses of ascitic fluid performed at the University of California Irvine Medical Center (UCIMC) from January 2003 to February 2006 were retrospectively retrieved. Corresponding EUS findings, cytology and histology slides, and follow‐up information were reviewed.RESULTS:One hundred one (101) cases were found. Two smears were submitted in 11 cases because of the scanty amount of fluid aspirated. In the remaining cases, 5 mL or less of fluid were aspirated in 56 patients, and, of 9 who had prior computed tomography (CT), ascitic fluid was not seen in 6. The cytologic diagnoses were as follows: 17 were positive for adenocarcinoma, 1 positive for metastatic small‐cell carcinoma of the lung, 1 positive for diffuse large‐cell lymphoma, 3 suspicious for adenocarcinoma, 1 suspicious for plasmacytoma, 4 atypical epithelial cells, and 74 negative. Cell block was available in 80 cases and immunohistochemical stains were performed in 71 cases to confirm the diagnosis. Six patients had peritoneal biopsy. The sensitivity, specificity, positive and negative predictive values, and diagnostic accuracy were 80%, 100%, 100%, 95%, and 96%, respectively.CONCLUSIONS:EUS‐guided paracentesis is a valuable aid in the cytologic diagnosis of malignant ascites. It is particularly useful when no abnormality is identified by CT. Cancer (Cancer Cytopathol) 2010;. © 2010 American Cancer Society.
Cellular features of psoriatic skin: Imaging and quantification using in vivo reflectance confocal microscopy
Authors: Wolberink E. A. W. , van Erp P. E. J. , Teussink M. M. , van de Kerkhof P. C. M. , Gerritsen M. J. P. 
Published: November 2010

AbstractBackground:In vivo reflectance confocal microscopy (RCM) is a novel, exciting imaging technique. It provides images of cell‐and tissue structures and dynamics in situ, in real time, without the need for ex vivo tissue samples. RCM visualizes the superficial part of human skin up to a depth of 250 μm. In psoriasis, an erythematosquamous skin disease, we evaluated well known histological features of stable psoriasis vulgaris (PP) with RCM. RCM images were correlated to morphological and cell biological findings in routine HE and immunohistochemical stained histology with CD3 and antifilaggrin antibodies.Methods:Lesional and nonlesional skin of eight patients with PP were evaluated with RCM, after which 4‐mm punch biopsies were taken and cut vertically in two equal parts. One part was processed in the conventional vertical way, the other horizontally (en face) for optimal correlation to RCM images. We evaluated and quantificated nine histopathological features of psoriasis: parakeratosis, epidermal and dermal inflammatory infiltrate, diminished or absent stratum granulosum, epidermal thickening, thinning of the suprapapillary epidermal plate, increased height of the papillary dermis, increase in number of dermal papillae and increase in number and volume of papillary capillaries.Results:Quantification and evaluation of cell biological and histological features of PP with RCM correlated highly to evaluation in HE, CD3 and filaggrin‐stained histology.Conclusions:RCM is a novel technique which can be used for real time, cytometric evaluation and quantification of PP features. RCM might be suited equally for cytometric evaluation of other superficial tissues. © 2010 International Clinical Cytometry Society
Activation of the nuclear receptor PPARα regulates lipid metabolism in foetal liver from diabetic rats: implications in diabetes‐induced foetal overgrowth
Authors: Martínez Nora , White Verónica , Kurtz Melisa , Higa Romina , Capobianco Evangelina , Jawerbaum Alicia 
Published: November 2010

AbstractBackgroundPeroxisome proliferator‐activated receptor α (PPARα) is a crucial regulator of liver lipid metabolism. As maternal diabetes impairs foetal lipid metabolism and growth, we aimed to determine whether PPARα activation regulates lipid metabolism in the foetal liver from diabetic rats as well as foetal weight and foetal liver weight.MethodsDiabetes was induced by neonatal streptozotocin administration (90 mg/kg). For ex vivo studies, livers from 21‐day‐old foetuses from control and diabetic rats were explanted and incubated in the presence of PPARα agonists (clofibrate and leukotriene B4) for further evaluation of lipid levels (by thin layer chromatography and densitometry), de novo lipid synthesis (by 14C‐acetate incorporation) and lipid peroxidation (by thiobarbituric reactive substances evaluation). For in vivo studies, foetuses were injected through the uterine wall with leukotriene B4 on days 19, 20 and 21 of gestation. On day 21 of gestation, foetal liver concentrations of lipids and lipoperoxides were evaluated.ResultsFoetuses from diabetic rats showed increased body weight and liver weight, as well as accumulation of triglycerides and cholesteryl esters, increased de novo lipid synthesis and lipid peroxidation in the liver when compared to controls. Ex vivo studies showed that PPARα ligands reduced both the concentrations and synthesis of the lipid species studied and lipid peroxidation in the foetal liver from diabetic rats. In vivo experiments showed that leukotriene B4 reduced the concentrations of triglycerides, cholesteryl esters and phospholipids, as well as lipid peroxidation, foetal weight and foetal liver weight in diabetic rats.ConclusionsPPARα activation regulates the impaired foetal liver lipid metabolism, prevents hepatomegaly and reduces foetal overgrowth induced by maternal diabetes. Copyright © 2010 John Wiley & Sons, Ltd.
Effects of the flaxseed oil on the fatty acid composition of tilapia heads
Authors: Aguiar Ana Carolina , Cottica Solange Maria , Boroski Marcela , Sargi Sheisa Cyléia , do Prado Ivanor Nunes , Bonafé Elton Guntendorfer , França Polyana Batoqui , de Souza Nilson Evelázio , Visentainer Jesuí Vergílio 
Published: November 2010

AbstractThe objective of this study was to evaluate the effects of adding flaxseed oil (FO) to feed on the incorporation of n‐3 PUFA in tilapia heads. Tilapia were given diets with increasing levels of FO (0.00, 1.25, 2.50, 3.75 and 5.00% for treatments A, B, C, D and E, respectively), as a source of LNA for 150 days. The proximate composition of the heads indicated high nutritional value and 40 FA (fatty acids) common to all treatments were identified in total lipids. Intake of LNA caused storage of LNA and sequential desaturation‐elongation to eicosapentaenoic acid (EPA) and DHA. With increasing levels of FO in the diet, the content of LNA in tilapia heads increased (1.7 and 14.0% for diets A and E, respectively), as well as the contents of EPA (0.1 and 0.9% for diets A and E, respectively) and DHA (0.5 and 1.8% for diets A and E, respectively). Adding FO to tilapia feed markedly increased the total content of n‐3 PUFA (3.0 and 21.1% for diets A and E, respectively), decreased the total content of n‐6 PUFA (41.3 and 24.9% for diets A and E, respectively), and consequently resulted in a decrease in the n‐6/n‐3 ratio (13.8 and 1.2 for diets A and E, respectively). Therefore, feeding tilapia with FO is a good way of valorizing this part of the fish by creating a valuable nutritional food source.
Determination of optical purity and absolute configuration of natural 12‐hydroxystearic acid by the 1H NMR anisotropy method
Authors: Murakami Satoshi , Satou Kazuhiro , Kijima Taturo , Watanabe Masataka , Izumi Taeko 
Published: November 2010

AbstractThe enantiopure of (R)‐(−) MαNPA was allowed to react with racemic 18‐(tert‐butyldimethylsilyloxy)‐5‐octadecayne‐7‐ol which was derived from dodecane‐1,12‐diol, yielding diastereomeric esters mixture. These racemic esters were easily separated by HPLC on silica‐gel. The absolute configurations of the first‐eluted diastereomeric esters from the separated esters were determined using 1H NMR anisotropy method. Analysis on 1H NMR spectra and HPLC elution time of the synthesized esters and those of MαNP ester derived from natural methyl‐12‐hydroxystealate showed that the absolute configuration of natural 12‐hydroxystearic acid was R form, and the enantiomeric excess was over 99%.
Four Novel Diterpenoids from Crinipellis sp. 113
Authors: Li Yao‐Yao , Shen Yue‐Mao 
Published: November 2010

AbstractFour novel diterpenoids, namely (4β)‐4,4‐O‐dihydrocrinipellin A (1), (4β,8α)‐4,4‐O,8,8‐O‐tetrahydrocrinipellin B (2), crinipellin C (3), and crinipellin D (4), along with three known ones, (3β,4β)‐3,3‐C,4,4‐O‐tetrahydrocrinipellin A (5), (4β)‐4,4‐O‐dihydrocrinipellin B (6), and phlebiakauranol alcohol, were isolated from the fungal strain Crinipellis sp. 113. Their structures were elucidated by spectroscopic analyses, including 1D‐ and 2D‐NMR experiments, and by HR‐Q‐TOF mass spectrometry. Antitumor and antibacterial assays with the novel compounds 1–4 were carried out, showing moderate activities against HeLa cells and no effects on the growth of tested bacteria or yeast.
One‐Pot Synthesis of Functionalized 2H‐Chromene (=2H‐1‐Benzopyran) Derivatives via a Three‐Component Reaction between a CH‐Acid, a Dialkyl Acetylenedicarboxylate, and Methyl Chloroglyoxylate or Benzyl Carbonochloridate Mediated by Triphenylphosphine
Authors: Bayat Mohammad , Shiraz Nader Zabarjad , Asayesh Soheila Shafei 
Published: November 2010

AbstractAn effective route to functionalized 2H‐chromene (=2H‐1‐benzopyran) derivatives 4 is described (Scheme 1). This involves the reaction of a 1,1‐diactivated alkene, resulting from the reaction of dimedone (=5,5‐dimethylcyclohexane‐1,3‐dione; 1a) with methyl chloroglyoxylate (ClC(O)COOMe), benzyl carbonochloridate (ClC(O)OCH2Ph) or 3,5‐dinitrobenzoyl chloride (3,5‐(NO2)2C6H3C(O)Cl), and a dialkyl acetylenedicarboxylate (=dialkyl but‐2‐ynedioate) in the presence of Ph3P which undergo intramolecular Wittig reaction to produce 2H‐chromene derivatives (Scheme 1).
A Novel Trypsin‐Catalyzed Three‐Component Mannich Reaction
Authors: Chai She‐Jie , Lai Yi‐Feng , Zheng Hui , Zhang Peng‐Fei 
Published: November 2010

AbstractWe found that the trypsin from hog pancreas displayed high activity to promote three‐component Mannich reaction of aromatic aldehydes, aromatic amines, and acetone with moderate‐to‐excellent yields. The reaction tolerates a great range of substrates and extends the application of trypsin in organic synthesis.
Phenolic Compounds from the Fresh Leaves of Eucalyptus maideni
Authors: Tian Li‐Wen , Yang Chong‐Ren , Zhang Ying‐Jun 
Published: November 2010

AbstractFour new phenolic compounds, 7‐O‐methylcatechin 5‐O‐β‐D‐glucopyranoside (1), 6‐O‐feruloyl‐D‐glucopyranose (2), demethylpiperitol 4‐O‐β‐D‐glucopyranoside (3), and 2‐episesaminol 2‐O‐β‐D‐glucopyranoside (4) were isolated from the fresh leaves of Eucalyptus maideni, together with six hydrolyzable tannins, 5–10, a flavonol glycoside, 11, three simple phenolics, 12–14, a monoterpene glucoside, 15, and a rosenoside, 16. Their structures were determined on the basis of detailed spectroscopic analysis, acidic hydrolysis, and enzymatic hydrolysis. The known compounds 10 and 13 were obtained from the genus Eucalyptus for the first time.
Presenegenin Glycosides from Securidaca welwitschii
Authors: Timité Gaoussou , Mitaine‐Offer Anne‐Claire , Miyamoto Tomofumi , Tanaka Chiaki , Paululat Thomas , Delaude Clément , Lacaille‐Dubois Marie‐Aleth 
Published: November 2010

AbstractThe five new presenegenin glycosides 1–5 were isolated from Securidaca welwitschii, together with one known sucrose diester. Compounds 1–4 were obtained as pairs of inseparable (E)/(Z)‐isomers of a 3,4‐dimethoxycinnamoyl derivative, i.e., 1/2 and 3/4. Their structures were elucidated mainly by 2D‐NMR techniques and mass spectrometry as 3‐O‐(β‐D‐glucopyranosyl)presenegenin 28‐{O‐β‐D‐xylopyranosyl‐(1→4)‐O‐α‐L‐rhamnopyranosyl‐(1→2)‐O‐[β‐D‐glucopyranosyl‐(1→3)]‐4‐O‐[(E)‐3,4‐dimethoxycinnamoyl]‐β‐D‐fucopyranosyl} ester (1) and its (Z)‐isomer 2, 3‐O‐(β‐D‐glucopyranosyl)presenegenin 28‐{O‐β‐D‐galactopyranosyl‐(1→4)‐O‐β‐D‐xylopyranosyl‐(1→4)‐O‐3‐O‐acetyl‐α‐L‐rhamnopyranosyl‐(1→2)‐O‐[β‐D‐glucopyranosyl‐(1→3)]‐4‐O‐[(E)‐3,4‐dimethoxycinnamoyl]‐β‐D‐fucopyranosyl} ester (3) and its (Z)‐isomer 4, and 3‐O‐(β‐D‐glucopyranosyl)presenegenin 28‐[O‐β‐D‐galactopyranosyl‐(1→3)‐O‐β‐D‐xylopyranosyl‐(1→4)‐O‐α‐L‐rhamnopyranosyl‐(1→2)‐β‐D‐fucopyranosyl] ester (5) (presenegenin=(2β,3β,4α)‐2,3,27‐trihydroxyolean‐12‐ene‐23,28‐dioic acid).
Synthesis, Physical Properties, and Cytotoxicity of Nitroxyl–Aziridine Hybrid
Authors: Kumamoto Takuya , Suzuki Kazuya , Kim Sang‐kook , Hoshino Katsuyoshi , Takahashi Masahiro , Sato Hiromi , Iwata Hiroki , Ueno Koichi , Fukuzumi Masahiro , Ishikawa Tsutomu 
Published: November 2010

AbstractNitroxyl–aziridine hybrid, a candidate for a magnetic resonance imaging (MRI) probe and an anticancer drug, was synthesized by aziridine formation reaction of nitroxyl‐introduced aldehyde and guanidinium ylide in good diastereoselectivity. The relative configuration at aziridine C(2)C(3) bond of the major diastereoisomer was determined to be cis by X‐ray crystallographic analysis. Application of chiral guanidinium ylide resulted in the formation of the corresponding optically active aziridine in 84% ee. Reversible one‐electron redox potential and quantitative spin yield of the hybrid were observed in cyclic voltammogram and electron spin resonance, respectively. However, cytotoxicity of the hybrid against cancer cell lines used was not observed.
Eleven New Triterpenes from Eurycorymbus cavaleriei
Authors: Cheng Lin , Shen Li‐Ming , Zhang Min , Li Ning , Li Xian , Ma Zhong‐Jun , Qu Hai‐Bin 
Published: November 2010

AbstractEleven new triterpenes, cavalerols A–K (1–11, resp.), ten of which were derivatives of hopane and one was derivative of dammarane, were isolated from the twigs of Eurycorymbus cavaleriei. Their structures were elucidated by spectroscopic methods including 2D‐NMR analysis. Cavalerol D (4) and cavalerol F (6), cavalerol B (2), and cavalerol G (7) were two pairs of isomers, and silver ion was introduced for their differentiation by multi‐stage tandem mass spectrometry. And nine compounds, except 5 and 10, were tested for quinone reductase (QR) induction activities in vitro, and results showed that compounds 2, 4, and 7 exhibited moderate induction activities with CD values of 8.62, 9.13, and 2.56 μg/ml, respectively, and compounds 6 and 8 showed cytotoxicity against hepa 1c1c7 cell line with IC50 values of no more than 1 μg/ml.
Sesquiterpenes and Other Metabolites from the Marine Red Alga Laurencia composita (Rhodomelaceae)
Authors: Ji Nai‐Yun , Li Xiao‐Ming , Wang Bin‐Gui 
Published: November 2010

AbstractTwo new halogenated sesquiterpenes, (8β)‐10‐bromo‐3‐chloro‐2,7‐epoxychamigr‐9‐en‐8‐ol (1) and 2‐bromo‐3‐chlorobisabola‐7(14),11‐diene‐6,10‐diol (3), and one new phytol‐derived diterpene, 2,3‐epoxyphytyl acetate (4), along with cis‐ and trans‐1‐methylcyclohexane‐1,4‐diol (5 and 6) which were isolated from a natural source for the first time but have been previously synthesized, were isolated from the marine red alga Laurencia composita and characterized. In addition, a known sesquiterpene, pacifenediol (2), and the known furanone derivative 7 were also identified. Their structures were established by NMR and mass spectroscopic methods.
Chemical Constituents of Phyllanthus reticulatus
Authors: Lan Ming‐Sheng , Ma Jian‐Xiong , Tan Chang‐Heng , Wei Song , Zhu Da‐Yuan 
Published: November 2010

AbstractA new purine derivative, 3‐(3‐methylbut‐2‐en‐1‐yl)isoguanine (1), and a new cleistanthane‐type diterpenoid glucoside, 19‐hydroxyspruceanol 19‐O‐β‐D‐glucopyranoside (2), together with eight known compounds were isolated from the whole plant of Phyllanthus reticulatus. The structures were elucidated by chemical and spectroscopic methods.
Safety of intranasal Bevacizumab (avastin) treatment in patients with hereditary hemorrhagic telangiectasia‐associated epistaxis
Authors: Chen Sonia , Karnezis Tom , Davidson Terence M. 
Published: November 2010

AbstractObjectives/Hypothesis:Assess for complications of intranasal Bevacizumab application in patients with hereditary hemorrhagic telangiectasia (HHT)‐associated epistaxis.Study Design:Retrospective chart review.Methods:In 58 patients presenting with recurrent HHT epistaxis, Bevacizumab was applied intranasally either as a submucosal injection or as a topical spray between October 2006 and June 2010. In many of the injected patients, the potassium titanyl phosphate (KTP) laser was used adjunctively for vessel photocoagulation. A phone interview was performed in July 2010 to assess for treatment complications.Results:Of the 58 treated patients 52 were contacted. Patient surveys were performed 1.5 to 46 months following their initial Bevacizumab treatment. Within the treatment population, five patients had sustained a septal perforation. Notably, these patients were treated early in the study period at which time the cartilaginous septum was often both injected and lasered. Subsequently, the treatment protocol was changed and the cartilaginous septum was neither lasered nor injected. After these changes were made no additional septal perforations were identified. No other adverse events were associated with intranasal Bevacizumab treatment.Conclusions:Bevacizumab applied as either a submucosal injection or as a topical nasal spray, with or without application of the KTP laser, is a safe treatment regimen. Still, when Bevacizumab injections are performed, the cartilaginous nasal septum should be avoided as patients may develop septal perforations. Laryngoscope, 2011
Long‐range 1H15N heteronuclear shift correlation across wide F1 spectral windows
Authors: Martin Gary E. , Hilton Bruce D. , Moskau Detlef , Freytag Nicolas , Kessler Klemens , Colson Kim 
Published: November 2010

AbstractLong‐range 1H15N heteronuclear shift correlation experiments at natural abundance are becoming more routinely utilized in the characterization of unknown chemical structures from a diverse range of sources including natural products and pharmaceuticals. Apart from the inherent challenges of the low gyromagnetic ratio and natural abundance of 15N, investigators are also occasionally hampered by having to deal with the wide spectral range inherent to various nitrogen functional groups, which can exceed 500 ppm. Earlier triple resonance cryoprobe designs typically provided 90° 15N pulses in the range of 35–40 µs, which did not allow the uniform excitation of wide F1 spectral ranges for 1H15N GHMBC spectra. We report the results obtained with a newly designed Bruker 600 MHz triple resonance TCI Micro CryoProbe™ using methyl orange as a model compound, in which the 15N resonances are separated by > 450 ppm. Copyright © 2010 John Wiley & Sons, Ltd.
Educational and vocational achievement among long‐term survivors of adolescent cancer in Germany
Authors: Dieluweit Ute , Debatin Klaus‐Michael , Grabow Desiree , Kaatsch Peter , Peter Richard , Seitz Diana C.M. , Goldbeck Lutz 
Published: November 2010

AbstractBackgroundAdolescence involves graduating from school and preparing one's professional career. The accomplishment of these tasks may be hampered by the experience of cancer. This study investigates the educational and professional achievements of German long‐term survivors of adolescent cancer.ProcedureAdult survivors of cancer during adolescence (n = 820, age at onset between 15 and 18 years; M = 15.8, SD = 0.9 years; age at follow‐up: M = 30.4, SD = 6.0 years) were recruited through the German Childhood Cancer Registry. They completed self‐reports with standard items on their educational and vocational level and their current occupational situation. Outcomes were compared to an age‐matched sample from the general population (German Socio‐Economic Panel, n = 820, age: M = 30.4, SD = 6.7). Risk factors for educational and vocational underachievement were identified by subgroup analyses.ResultsCompared to peers from the general population, survivors of cancer during adolescence achieved higher educational and vocational levels. A higher proportion of survivors was employed; however, survivors were significantly older when starting their first occupation. Subgroup analyses revealed that neuropsychological late effects were associated with reduced rates of graduation from university and of employment among the survivors. No such effect of neuro‐cognitive late effects occurred for high school graduation.ConclusionsMost German survivors of cancer during adolescence participate in school and vocational life without major difficulties. Problems particularly arise for survivors with neuropsychological sequelae. Further research should investigate whether these results can be attributed to the German support system for pediatric cancer patients or to sample effects. Pediatr Blood Cancer 2011;56:432–438. © 2010 Wiley‐Liss, Inc.
Survival by race among children with extracranial solid tumors in the United States between 1985 and 2005
Authors: Johnson Kelsey A. , Aplenc Richard , Bagatell Rochelle 
Published: November 2010

AbstractBackgroundThe National Cancer Institute's Surveillance, Epidemiology, and End Results (SEER) database was used to compare survival rates by race among children with common extracranial solid tumors between 1985 and 2005.ProcedureDiagnosis and outcome data were obtained from SEER. Five‐year survival probabilities were calculated using the Kaplan–Meier method. Survival hazard ratios were calculated using the Cox proportional hazards model. Survival probabilities were compared among Whites, Blacks, American Indians/Alaskan Natives, and Asians/Pacific Islanders.ResultsFive‐year survival probabilities were higher for White children than for children belonging to other racial groups (77% vs. 71% for Blacks, 72% for American Indian/Alaskan Native, 72% for Asian/Pacific Islander). Male non‐White children had worse 5‐year survival than male White children while there were no differences in survival among females across racial groups. There was no difference when survival probabilities for Hispanic and non‐Hispanic children were compared. Overall, Black children had a higher risk of death compared to White children (1.31, P < 0.05). Black children had a higher risk of death from germ cell tumors, hepatoblastoma and non‐rhabdomyosarcoma soft tissue sarcomas. Asian/Pacific Islander children also had a higher risk of death overall (1.34, P < 0.05) and a higher risk of death from germ cell tumors, hepatocellular carcinoma, neuroblastoma, and Wilms tumor compared to White children.ConclusionsMale children from minority groups have poorer survival from extracranial solid malignancies than White male children. Future efforts should be directed at understanding the causes of these differences and at developing practical clinical interventions to eliminate them. Pediatr Blood Cancer 2011;56:425–431. © 2010 Wiley‐Liss, Inc.