bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of thermophilic and mesophilic cultures blended with buffalo milk on camel milk cheese quality and proteolysis
Authors: Qadeer Zahida , Huma Nuzhat , Rehman Muhammad Adil , Ahmad Baila , Hafeez Hammad , Ali Umair , Shah Faiz‐ul‐Hassan , Tariq Muhammad Rizwan , Nayik Gulzar Ahmad , Ramniwas Seema , Salmen Saleh H. , Ansari Mohammad Javed 
Published: June 2024

SummaryCamel milk and its products are gaining higher demand due to their attractive nutritional and therapeutic profile. However, manufacturing of camel milk cheese (CMC) is a challenging task. This study aimed to compare the quality of camel milk cheese made using different starter cultures. Four groups CM (camel milk + mesophilic cultures), CT (camel milk + thermophilic cultures), CBM (camel milk + 10% buffalo milk using mesophilic cultures), and CBT (camel milk + 10% buffalo milk using thermophilic cultures) were created. The cheeses were stored for 60 days at 4 °C to assess their physicochemical, sensory, and proteolytic properties. Results indicated highest moisture content in CM (70.19%) as opposed to the lowest moisture in CBT (55.19%). In addition, CM and CBM revealed lower acidity (0.63% and 0.66%) in contrast with CT and CBT (0.78% and 0.83%, respectively). On the other hand, CBM and CBT exhibited higher contents of protein (21.04, 21.57%) and fat (17.65, 17.70%) during storage. All quality indices of cheese samples significantly declined (P < 0.01) during storage excluding acidity. Sensory analyses revealed that panellists highly preferred CBT treatment followed by CBM and CT. Furthermore, urea‐PAGE and RP‐HPLC were performed for the proteolytic study of cheese samples. The study revealed that the presence of buffalo milk and thermophilic cultures in cheese resulted in a decrease concentration of intact caseins (αs1, β‐, and κ‐caseins) as storage days passed. The degree of proteolysis was found to be higher in CBT compared to CBM, CT, and CM. Based on the results, it was concluded that the use of pasteurisation at 65 °C for 30 min, pH of 5.5, 0.06% CaCl2, and the incorporation of 10% buffalo milk using thermophilic cultures (Lactobaccilus bulgaricus and Streptococcus thermophilus) resulted in desirable quality characteristics.
The mediating effect of coping styles between emergency capacity and mental workload among clinical nurses: A cross‐sectional study
Authors: Jin Man , Qian Rong , Wang Jialin , Yuan Zhongqing , Long Juan , Zeng Li , Yang Huawu , Liao Dan , Xie Yunhui , Liu Xu 
Published: June 2024

AbstractAimsThis study aimed to examine the relationship between emergency capacity, coping styles, and mental workload among nurses.BackgroundEmergency capacity, coping styles, and mental workload are all variables associated with work. Identifying the relationship between these variables can facilitate administrators to implement tailored and effective intervention strategies to improve individual performance, quality of care, and medical safety.MethodsA quantitative cross‐sectional study was carried out to investigate 605 Chinese clinical nurses in seven tertiary hospitals by using personal information form, emergency capacity scale for nurses, simplified coping skill questionnaire, and the NASA‐Task Load Index.ResultsEmergency capacity and mental workload were found at moderate levels. The multiple linear regression model suggested that spinsterhood, no children, high workload, always anxiety or nervousness, and lower monthly income were the influencing factors of mental workload. Positive coping style was positively correlated with emergency capacity and negatively correlated with mental workload. Negative coping style was negatively related to emergency capacity and positively related to mental workload. Additionally, coping styles played a partial mediating role in the relationship between emergency capacity and mental workload through constructing a structural equation model, but the effects of positive coping style and negative coping style are opposite.ConclusionOur results showed that coping styles played a mediating role in the relationship between emergency capacity and mental workload. Managers can alleviate the mental workload of nurses by cultivating positive coping styles and improving emergency capacity.Implications for nursing and nursing policyMental workload of nurses deserves more attention in medical institutions. The results of our study provide evidence for improving employee health, promoting positive behaviors, and optimizing organizational management. Nursing managers should take feasible measures to fulfill nurses’ needs for emergency capacity and coping strategies to alleviate nurses' mental workload, so as to stimulate their intrinsic motivation and positive organizational behavior.
Investigation of theoretical infrared spectra on microwave dielectric properties of Re2O3 (Re = Ho, Er, Tm) ceramics
Authors: Ma Shu‐Yang , Xia Wang‐Suo , Shi Ming‐An , Wang Ying , Chen Lei‐Ming , Wu Hai‐Tao 
Published: June 2024

AbstractBased on the crystal structure data of Re2O3 (Re = Ho, Er, Tm) ceramics prepared by the solid‐phase reaction method, the theoretical infrared spectra were calculated via the first‐principles method and were first used to investigate the intrinsic influence of microwave dielectric properties. In addition, the phonon spectra, density of states, and electron localization function (ELF) were also calculated to analyze the dynamic stability and bonding properties. Based on the simple damped harmonic oscillator model, the resonant frequency of Re2O3 (Re = Ho, Er, Tm) ceramics in infrared spectrum varied in accordance with the ELF values because the restoring force constant of the elastic term was determined by the bonding properties. The intensity of the resonant peak was determined by the effective charge and decreased as the ELF increased. The full width at half maximum (FWHM) was dominated by the damping coefficient and had no obvious relationship with bond properties. For microwave dielectric properties, the dielectric constant of Re2O3 (Re = Ho, Er, Tm) ceramics was in accordance with the characteristic peak intensity of the theoretical infrared spectra, and the quality factor (Q × ƒ) was mainly determined by FWHM, corresponding to the damping in lattice vibration.
Proteomic analysis of exosomes derived from fatty hepatocytes of grass carp
Authors: Lu Ronghua , Yang Lulu , Jia Shenzong , Zhang Yuru , Xu Xinxin , Cao Xianglin , Sun Jian 
Published: June 2024

AbstractExosomes participate in intercellular communication by carrying proteins, messenger RNA, microRNAs, and non‐coding RNA. Fatty liver is a common phenomenon in farmed fish, but there has been little study of fatty hepatocytes‐derived exosomes. Here, we successfully isolated exosomes from hepatocytes of grass carp, named Exos (hepatocytes‐derived exosomes) and OA‐Exos (fatty hepatocytes‐derived exosomes), from which 617 differentially expressed proteins were identified using liquid chromatography tandem mass spectrometry. Of these, 320 proteins were promoted and 297 proteins were restrained, which were gathered in biological processes and cellular components (cellular processes, cells, and intracellular structures). The results of kyoto encyclopedia of genes and genomes (KEGG) analysis revealed that the differential expression proteins were gathered in “carbohydrate transport and metabolism”, “translation, ribosomal structure and biogenesis”, “posttranslational modification, protein turnover, chaperones”, and “intracellular trafficking, secretion, and vesicular transport”. In addition, five differentially expressed exosomal proteins were further confirmed by parallel reaction monitoring, including 2‐phospho‐D‐glycerate hydrolyase, cytochrome b5, fatty acid‐binding protein domain‐containing protein, metallothionein, and malate dehydrogenas, which were downregulated. These findings provided evidence that exosomes derived from fatty hepatocytes of grass carp may be biomarkers for the early diagnosis, treatment, and prevention of fatty liver in fishery development.
Bioisosteric analogs of MDMA: Improving the pharmacological profile?
Authors: Alberto‐Silva Ana Sofia , Hemmer Selina , Bock Hailey A. , da Silva Leticia Alves , Scott Kenneth R. , Kastner Nina , Bhatt Manan , Niello Marco , Jäntsch Kathrin , Kudlacek Oliver , Bossi Elena , Stockner Thomas , Meyer Markus R. , McCorvy John D. , Brandt Simon D. , Kavanagh Pierce , Sitte Harald H. 
Published: June 2024

Abstract3,4‐Methylenedioxymethamphetamine (MDMA, ‘ecstasy’) is re‐emerging in clinical settings as a candidate for the treatment of specific neuropsychiatric disorders (e.g. post‐traumatic stress disorder) in combination with psychotherapy. MDMA is a psychoactive drug, typically regarded as an empathogen or entactogen, which leads to transporter‐mediated monoamine release. Despite its therapeutic potential, MDMA can induce dose‐, individual‐, and context‐dependent untoward effects outside safe settings. In this study, we investigated whether three new methylenedioxy bioisosteres of MDMA improve its off‐target profile. In vitro methods included radiotracer assays, transporter electrophysiology, bioluminescence resonance energy transfer and fluorescence‐based assays, pooled human liver microsome/S9 fraction incubations, metabolic stability studies, isozyme mapping, and liquid chromatography coupled to high‐resolution mass spectrometry. In silico methods included molecular docking. Compared with MDMA, all three MDMA bioisosteres (ODMA, TDMA, and SeDMA) showed similar pharmacological activity at human serotonin, dopamine, and norepinephrine transporters (hSERT, hDAT, and hNET, respectively) but decreased agonist activity at 5‐HT2A/2B/2C receptors. Regarding their hepatic metabolism, they differed from MDMA, with N‐demethylation being the only metabolic route shared, and without forming phase II metabolites. In addition, TDMA showed an enhanced intrinsic clearance in comparison to its congeners. Additional screening for their interaction with human organic cation transporters (hOCTs) and plasma membrane monoamine transporter (hPMAT) revealed a weaker interaction of the MDMA analogs with hOCT1, hOCT2, and hPMAT. Our findings suggest that these new MDMA bioisosteres might constitute appealing therapeutic alternatives to MDMA, sparing the primary pharmacological activity at hSERT, hDAT, and hNET, but displaying a reduced activity at 5‐HT2A/2B/2C receptors and alternative hepatic metabolism. Whether these MDMA bioisosteres may pose lower risk alternatives to the clinically re‐emerging MDMA warrants further studies.image
Specialized medical care for people with intellectual disabilities: A retrospective cohort study in an outpatient ID practice
Authors: Breuer Marian E. J. , Naaldenberg Jenneken , Schalk Bianca W. M. , Heutmekers Marloes , Pelle Tim , Bakker‐van Gijssel Esther J. , Leusink Geraline L. 
Published: June 2024

AbstractPeople with intellectual disabilities (ID) experience complex medical care needs and high levels of multimorbidity. In mainstream healthcare, these needs might remain undetected leading to unmanaged health problems and avoidable deaths. In the Netherlands, general practitioners (GPs) can refer to specialized ID physicians when ID‐specific expertise is required. Little is known about the characteristics of specialized medical care for people with ID. This study explores the characteristics of specialized medical care for people with ID, including the interplay between medical‐, psychological‐, and context‐related problems. A retrospective cohort study using medical records of patients with ID who had visited the outpatient ID practice in Nijmegen, the Netherlands. Medical records (n = 128) were analyzed using descriptive statistics, focusing on (1) reasons for initial consultation, (2) health‐related problems identified during initial consultation, and (3) disciplines involved following initial consultation. Analyses were performed separately for patients who were referred by a medical professional and patients who visited the practice for proactive health checks related to the etiological diagnosis. Patients often initially visit the outpatient ID practice for one type of complaint, most often psychological. Diverse, multiple, and interconnected problems were identified during specialized medical ID consultation. A range of specialist professionals (n = 25) were involved by the ID physician. The health‐related problems of people with ID seen at the outpatient ID practice are diverse and interconnected, and originate from an interplay between medical‐, psychological‐, and context‐related problems. This complexity is not mirrored in the reasons for referring to the outpatient ID practice. It is essential to go beyond medical views and assess health complaints in an integrated way, including the way ID can influence all levels (physical, mental, contextual) of the experienced health issue within the context of everyday life.
Contents and effectiveness of patient‐ and family‐centred care interventions in adult intensive care units: A systematic review
Authors: Joo Youngshin , Jang Yeonsoo , Kwon Oh Young 
Published: June 2024

AbstractBackgroundThe need and values of patient‐ and family‐centred care (PFCC) have been globally increasing in the health care landscape. However, the concept of PFCC and the components in adult intensive care units (ICUs) remain wide‐ranging.AimTo elucidate the core concepts of PFCC interventions and evaluate the effects of the interventions in adult ICUs.Study DesignWe searched electronic databases (PubMed, Cochrane Central, CINAHL, EMBASE, PsycINFO, RISS, KMbase and KoreaMed) from inception to 20 June 2022, for all studies on PFCC interventions. Three authors independently conducted data screening and extraction. The core concepts and the effects of PFCC interventions in adult ICUs were examined. The effects of patient‐ and family‐centred care interventions in adult ICUs were examined. The quality of the included studies was evaluated using the Mixed Methods Appraisal Tool.ResultsOverall, 3507 records were identified, and 14 full‐text articles were assessed. Participants in the included studies were patients and/or their family members in adult ICUs. The main concepts of the studies were participation and information‐sharing. Only two studies used collaboration as the main concept of intervention. PFCC interventions have shown positive outcomes for patients, including increased satisfaction, improvement of patient health status and reduced incidence of complications. They have also been beneficial for families, leading to higher satisfaction levels and decreased anxiety. Additionally, these interventions have positively impacted health care providers by enhancing satisfaction and improving rounding efficiency. Moreover, they have influenced health care utilization by decreasing hospital costs and length of stay.ConclusionsThis review highlights the advantages of PFCC interventions for patients, families and health care providers in adult ICUs. Future research should focus on developing strategies to incorporate collaboration more comprehensively as a core concept in the implementation of PFCC interventions.Relevance to Clinical PracticeFuture research endeavours must prioritize collaborative efforts involving health care providers, patients and their families by deploying an array of strategies within the intensive care unit setting.
Production of grains with ultra‐low heavy metal accumulation by pyramiding novel Alleles of OsNramp5 and OsLsi2 in two‐line hybrid rice
Authors: Hu Shikai , Zhou Liang , Wang Jingxin , Mawia Amos Musyoki , Hui Suozhen , Xu Bo , Jiao Guiai , Sheng Zhonghua , Shao Gaoneng , Wei Xiangjin , Wang Ling , Xie Lihong , Zhao Fengli , Tang Shaoqing , Hu Peisong 
Published: June 2024

SummaryEnsuring rice yield and grain safety quality are vital for human health. In this study, we developed two‐line hybrid rice (TLHR) with ultra‐low grain cadmium (Cd) and arsenic (As) accumulation by pyramiding novel alleles of OsNramp5 and OsLsi2. We first generated low Cd accumulation restorer (R) lines by editing OsNramp5, OsLCD, and OsLCT1 in japonica and indica. After confirming that OsNramp5 was most efficient in reducing Cd, we edited this gene in C815S, a genic male sterile line (GMSL), and screened it for alleles with low Cd accumulation. Next, we generated R and GMSL lines with low As accumulation by editing OsLsi2 in a series of YK17 and C815S lines. When cultivated in soils that were heavily polluted with Cd and As, the edited R, GMSL, and TLHR plants showed significantly reduced heavy metal accumulation, while maintaining a relatively stable yield potential. This study provides an effective scheme for the safe production of grains in As‐ and/or Cd‐polluted paddy fields.
Harold Ickes: A New Dealer's opposition to the early Cold War
Authors: Shaffer Robert 
Published: June 2024

AbstractHarold Ickes, an important New Deal policy‐maker, was also, after he left his post as Interior Secretary, a vocal critic of President Harry Truman's Cold War policy. His criticisms – distributed first through a thrice‐weekly syndicated column and then in a weekly magazine column – most significantly identified United States naval and military policies in its Pacific territories as both unjust to their Indigenous inhabitants and as sources of global tensions. In particular, Ickes in the late 1940s challenged Americans to see their nation's dealings abroad as the rest of the world did: as too often militaristic, undemocratic, and hypocritical. While no apologist for Soviet policy, Ickes primarily framed postwar international relations through his opposition to militarism and fascism rather than through a Communist/non‐Communist dichotomy. As it broadens the pantheon of American Cold War critics, this study also contributes to recent scholarship emphasizing interconnections between US colonial control over its offshore territories and US foreign relations.
Relational properties: Definition, reduction, and states of affairs
Authors: Meinertsen Bo R. 
Published: June 2024

AbstractThis paper defines relational properties and argues for their reducibility in a, broadly speaking, Armstrongian framework of state of affairs ontology and truthmaking. While Armstrong's own characterisation and reduction of them arguably is the best one available in the literature of this framework, it suffers from two main problems. As will be shown, it neither defines relational properties very clearly (if at all), nor provides an adequate conception of their reduction. This paper attempts to remedy this situation in four steps. First, it introduces relational properties and why they matter in metaphysics in general. Second, by briefly comparing and contrasting them with extrinsic properties, and by briefly mapping them onto a tripartite division of relations, the particular kinds of relevant relational properties are identified. Third, it classifies relational properties and thereby makes possible an apt definition of them. Finally, using the notion of truthmaking, it outlines a more satisfactory case for their reduction.
Transient receptor potential mucolipin 1 circumvents oxidative stress in primary human melanocytes
Authors: Chen Yi , Xie Bo , Hu Yebei , Sun Jiayi , Xu Jinhui , Shen Yuqing , Zhu Yuqi , Song Xiuzu 
Published: June 2024

AbstractBackgroundTransient Receptor Potential Mucolipin 1 (TRPML1) serves as a pivotal reactive oxygen species (ROS) sensor in cells, which is implicated in the regulation of autophagy. However, its function in melanocyte autophagy under oxidative stress remains elusive.MethodsThe expression and ion channel function of TRPML1 were investigated using immunofluorescence and calcium imaging in primary human melanocytes (MCs). After activating TRPML1 with MLSA1 (TRPML1 agonist), autophagy‐related molecules were investigated via western blot. ROS level, apoptosis‐ and autophagy‐related molecules were investigated after pretreatment with MLSA1. After interference with TRPML1 expression, mitochondrial structures were visualized by electron microscopy with hydrogen peroxide (H2O2）treatment.ResultsTRPML1 was expressed and functionally active in primary human MCs, and its activation promotes elevated expression of LC3‐II and reduced apoptosis and ROS levels under oxidative stress. TRPML1 downregulation caused mitochondrial swelling and disruption of cristae structures under oxidative stress in primary human MCs.ConclusionsTRPML1 might mediate lysosomal autophagy in primary human MCs under oxidative stress, participating in mechanisms that maintain the oxidative and antioxidant systems in balance.
Identification of common mechanisms and biomarkers for dermatomyositis and atherosclerosis based on bioinformatics analysis
Authors: Ma Yirong , Lai Junyu , Wan Qiang , Chen Zhengtao , Sun Liqiang , Zhang Qinhe , Guan Chengyan , Li Qiming , Wu Jianguang 
Published: June 2024

AbstractBackgroundDermatomyositis (DM) manifests as an autoimmune and inflammatory condition, clinically characterized by subacute progressive proximal muscle weakness, rashes or both along with extramuscular manifestations. Literature indicates that DM shares common risk factors with atherosclerosis (AS), and they often co‐occur, yet the etiology and pathogenesis remain to be fully elucidated. This investigation aims to utilize bioinformatics methods to clarify the crucial genes and pathways that influence the pathophysiology of both DM and AS.MethodMicroarray datasets for DM (GSE128470, GSE1551, GSE143323) and AS (GSE100927, GSE28829, GSE43292) were retrieved from the Gene Expression Omnibus (GEO) database. The weighted gene co‐expression network analysis (WGCNA) was used to reveal their co‐expressed modules. Differentially expression genes (DEGs) were identified using the “limma” package in R software, and the functions of common DEGs were determined by functional enrichment analysis. A protein‐protein interaction (PPI) network was established using the STRING database, with central genes evaluated by the cytoHubba plugin, and validated through external datasets. Immune infiltration analysis of the hub genes was conducted using the CIBERSORT method, along with Gene Set Enrichment Analysis (GSEA). Finally, the NetworkAnalyst platform was employed to examine the transcription factors (TFs) responsible for regulating pivotal crosstalk genes.ResultsUtilizing WGCNA analysis, a total of 271 overlapping genes were pinpointed. Subsequent DEG analysis revealed 34 genes that are commonly found in both DM and AS, including 31 upregulated genes and 3 downregulated genes. The Degree Centrality algorithm was applied separately to the WGCNA and DEG collections to select the 15 genes with the highest connectivity, and crossing the two gene sets yielded 3 hub genes (PTPRC, TYROBP, CXCR4). Validation with external datasets showed their diagnostic value for DM and AS. Analysis of immune infiltration indicates that lymphocytes and macrophages are significantly associated with the pathogenesis of DM and AS. Moreover, GSEA analysis suggested that the shared genes are enriched in various receptor interactions and multiple cytokines and receptor signaling pathways. We coupled the 3 hub genes with their respective predicted genes, identifying a potential key TF, CBFB, which interacts with all 3 hub genes.ConclusionThis research utilized comprehensive bioinformatics techniques to explore the shared pathogenesis of DM and AS. The three key genes, including PTPRC, TYROBP, and CXCR4, are related to the pathogenesis of DM and AS. The central genes and their correlations with immune cells may serve as potential diagnostic and therapeutic targets.