bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nanostructured Membranes for Enzyme Catalysis and Green Synthesis of Nanoparticles
Authors: Smuleac Vasile , Varma Rajender , Baruwati Babita , Sikdar Subhas , Bhattacharyya Dibakar 
Published: November 2011

AbstractMacroporous membranes functionalized with ionizable macromolecules provide promising applications in high capacity toxic metal capture, nanoparticle syntheses, and catalysis. Our low‐pressure membrane approach has good reaction and separation selectivities, which are tunable by varying pH, ionic strength, or pressure. The sustainable green chemistry approach under ambient conditions and the evaluation of a reactive poly(acrylic acid) (PAA)‐modified polyvinylidene fluoride (PVDF) membrane is described. Two distinct membrane types were obtained through different methods: 1) a stacked membrane through layer‐by‐layer assembly for the incorporation of enzymes (catalase and glucose oxidase), providing tunable product yields and 2) Fe/Pd nanoparticles for degradation of pollutants, obtained through an in situ green synthesis. Bioreactor–nanodomain interactions and mixed matrix nanocomposite membranes provide remarkable versatility compared to conventional membranes.
Electrochemical Synthesis of Indium(0) Nanoparticles in Haloindate(III) Ionic Liquids
Authors: Estager Julien , Nockemann Peter , Seddon Kenneth R. , Srinivasan Geetha , Swadźba‐Kwaśny Małgorzata 
Published: November 2011

AbstractA synthetic route to indium(0) nanoparticles via an electrochemical reduction of haloindate(III) ionic liquids to indium(I), and its subsequent disproportionation to indium(0) and indium(III) in the bulk electrolyte, is described. In this sustainable method, the ionic liquid acts simultaneously as metal source, templating agent, and stabilising agent, with the electron as the only reducing agent. The nature of the ionic liquid cation is demonstrated to strongly affect the morphology and size distribution of the indium(0) nanoparticles.
Extracellular matrix remodeling accompanies axial muscle development and morphogenesis in the mouse
Authors: Deries Marianne , Gonçalves André B. , Vaz Raquel , Martins Gabriel G. , Rodrigues Gabriela , Thorsteinsdóttir Sólveig 
Published: November 2011

AbstractBackground: Skeletal myogenesis is extensively influenced by the surrounding environment. However, how the extracellular matrix (ECM) affects morphogenesis of muscles is not well understood. Results: We mapped the three‐dimensional (3D) organization of fibronectin, tenascin, and laminin by immunofluorescence during early epaxial myogenesis in mouse embryos. We define four stages of dermomyotome/myotome development and reveal the 3D organization of myogenic cells within their ECM during those stages. Fibronectin is abundant in all interstitial tissues, while tenascin is restricted to intersegmental borders. Bundles of fibronectin and tenascin also penetrate into the myotome, possibly promoting myocyte alignment. A laminin matrix delineates the dermomyotome and myotome and undergoes dynamic changes, correlating with key developmental events. Conclusion: Our observations cast new light on how myotomal cells interact with their environment and suggest that, as the segmented myotomes transform into the epaxial muscle masses, the laminin matrix disassembles and myocytes use the abundant fibronectin matrix to reach their final organization. Developmental Dynamics 241:350–364, 2012. © 2011 Wiley Periodicals, Inc.
Summary of ‘Probiotics for treating persistent diarrhoea in children’
Authors: Editorial Office Evidence‐Based Child Health 
Published: November 2011

AbstractThis is a summary of Cochrane review, published in this issue of EBCH, first published as: Bernaola Aponte G, BadaMancilla CA, Carreazo Pariasca NY, Rojas Galarza RA. Probiotics for treating persistent diarrhoea in children. Cochrane Database of Systematic Reviews 2010, Issue 11. Art. No.: CD007401. DOI: 10.1002/14651858.CD007401.pub2 Copyright © 2011 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. The Cochrane Collaboration
Summary of ‘Probiotics for treating acute infectious diarrhoea’
Authors: Editorial Office Evidence‐Based Child Health 
Published: November 2011

AbstractThis is a summary of Cochrane review, published in this issue of EBCH, first published as: Allen SJ, Martinez EG, Gregorio GV, Dans LF. Probiotics for treating acute infectious diarrhoea. Cochrane Database of Systematic Reviews Evidence‐Based Child Health Editorial Office2010, Issue 11. Art. No.: CD003048. DOI: 10.1002/14651858.CD003048.pub3. Copyright © 2011 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. The Cochrane Collaboration
Summary of ‘Anti‐pseudomonal beta‐lactams for the initial, empirical, treatment of febrile neutropenia: comparison of beta‐lactams’
Authors: Editorial Office Evidence‐Based Child Health 
Published: November 2011

AbstractThis is a summary of Cochrane review, published in this issue of EBCH, first published as: Paul M, Yahav D, Bivas A, Fraser A, Leibovici L. Anti‐pseudomonal beta‐lactams for the initial empirical treatment of febrile neutropenia: comparison of beta‐lactams. Cochrane Database of Systematic Reviews Evidence‐Based Child Health Editorial Office2010, Issue 11. Art. No.: CD005197. DOI: 10.1002/14651858.CD005197.pub3. Copyright © 2011 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. The Cochrane Collaboration
Copper‐Catalyzed Synthesis of 4‐Aryl‐1H‐1,2,3‐triazoles from 1,1‐Dibromoalkenes and Sodium Azide
Authors: Wang Xiaokun , Kuang Chunxiang , Yang Qing 
Published: November 2011

AbstractA new methodology for the Cu‐catalyzed synthesis of 1H‐1,2,3‐triazoles from 1,1‐dibromoalkenes and sodium azide is presented. Aryl dibromoolefins were efficiently converted into the corresponding 1,2,3‐triazoles. A comprehensive number of functional groups were compatible with this reaction. 1,2,3‐Triazoles were obtained in moderate to excellent yields.
Differences in Serum Zn Levels in Acutely Ill and Recovered Adolescents and Young Adults with Anorexia Nervosa – A Pilot Study
Authors: Zepf Florian Daniel , Sungurtekin Idil , Glass Franziska , Elstrodt Linda , Peetz Dirk , Hintereder Gudrun , Kratzsch Jürgen , Poustka Fritz , Herpertz‐Dahlmann Beate , Wöckel Lars 
Published: November 2011

AbstractPreliminary evidence suggests that changes in zinc (Zn) metabolism are associated with anorexia nervosa (AN). However, data are scarce regarding potential differences in serum Zn concentrations in adolescent and young adult patients with AN. It was the aim of the present pilot study to compare serum Zn concentrations between acutely ill and remitted adolescent and young adult female patients with AN and female controls. Zn concentrations were higher in remitted compared with acutely ill patients. Zn concentrations were also higher in remitted patients compared with controls, but there was no significant difference in Zn concentrations between acutely ill patients and controls. The present study provides preliminary evidence for differences in serum Zn status in recovered patients with AN. These differences are likely influenced by reported food preferences, in particular as regards Ca2+ and phosphorus‐containing foods. However, because of limited statistical power, future research involving larger samples is necessary. Copyright © 2011 John Wiley & Sons, Ltd and Eating Disorders Association.
Screening serum biomarkers for early primary hepatocellular carcinoma using a phage display technique
Authors: Zhang Zhejia , Xu Linyong , Wang Zhiming 
Published: November 2011

AbstractHepatocellular carcinoma (HCC) occurs mainly in chronically diseased livers following hepatitis B virus (HBV) or hepatitis C virus (HCV) infection. Early detection and diagnosis of HCC would be of great clinical benefit. In this study, we used a random phage display peptide library and sera from early‐stage primary HCC patients (n = 30) to screen potential serum biomarkers for early primary HCC. Age‐ and sex‐matched patients with HBV and/or HCV infection were used as controls. In the screening phase, 19 out of 20 randomly selected phage clones exhibited specific reaction with purified sera IgG from early primary HCC patients, among them 14 coming from the same phage clone with inserted peptidesequence RGWCRPLPKGEG (named HC1). In the validation phase, phage ELISA results showed that the positive reaction rate of the HC1 phage clone was 91.4% with the early HCC group (n = 70), significantly higher than that with the HBV infection group (20.0%) (n = 70), the HCV infection group (12.9%) (n = 70), the HBV + HCV infection group (24.3%) (n = 70), the cirrhosis group (17.1%) (n = 70), and the healthy control group (10.0%) (n = 70). In conclusion, the HC1 mimic peptide showed high diagnostic validity for early primary HCC, and thereby could be a candidate serum biomarker for early primary HCC. J. Clin. Lab. Anal. 25:402–408, 2011. © 2011 Wiley Periodicals, Inc.
Speckle reduction imaging of breast ultrasound does not improve the diagnostic performance of morphology‐based CAD System
Authors: Tseng Hsin‐Shun , Wu Hwa‐Koon , Chen Shou‐Tung , Kuo Shou‐Jen , Huang Yu‐Len , Chen Dar‐Ren 
Published: November 2011

AbstractPurpose.Speckle reduction imaging (SRI) is a newly developed technique in ultrasound examination. This study aimed to compare the diagnostic performance of SRI and non‐SRI breast ultrasound examinations by using a morphology‐based computer‐aided diagnostic system.Methods.One hundred ten patients with pathologically proven breast lesions were enrolled consecutively from April 2008 to October 2008. SRI and non‐SRI ultrasound images were both obtained at the same examination for each patient. The regions of interest were manually sketched by an experienced physician without histological information. Nineteen practical morphologic features from the extracted contour were calculated and a support vector machine classifier identified the breast tumor as benign or malignant. Conventional binomial receiver operating characteristics curve analysis was used to represent the diagnostic performance of both SRI and non‐SRI.Results.Between SRI and non‐SRI methods, there were no significant differences in the area under the receiver operating characteristics curve (Az value: 0.82 versus 0.81), the sensitivity (78.9% versus 84.2%), and the specificity (73.6% versus 70.8%).Conclusions.Based on the morphology study, the performance of breast ultrasound in characterizing the solid breast mass as benign or malignant was not significantly improved with SRI. © 2011 Wiley Periodicals, Inc. J Clin Ultrasound, 2011;
Hospice eligibility in patients who died in a tertiary care center
Authors: Freund Katherine , Weckmann Michelle T. , Casarett David J. , Swanson Kristi , Brooks Mary Kay , Broderick Ann 
Published: November 2011

AbstractBACKGROUND:Hospice is a service that patients, families, and physicians find beneficial, yet a majority of patients die without receiving hospice care. Little is known about how many hospitalized patients are hospice eligible at the time of hospitalization.METHODS:Retrospective chart review was used to examine all adult deaths (n = 688) at a tertiary care center during 2009. Charts were selected for full review if the death was nontraumatic and the patient had a hospital admission within 12 months of the terminal admission. The charts were examined for hospice eligibility based on medical criteria, evidence of a hospice discussion, and hospice enrollment.RESULTS:Two hundred nine patients had an admission in the year preceding the terminal admission and a nontraumatic death. Sixty percent were hospice eligible during the penultimate admission. Hospice discussions were documented in 14% of the hospice‐eligible patients. Patients who were hospice eligible had more subspecialty consults on the penultimate admission compared to those not hospice eligible (P = 0.016), as well as more overall hospitalizations in the 12 months preceding their terminal admission (P = 0.0003), and fewer days between their penultimate admission and death (P = 0.001).CONCLUSION:The majority of terminally ill inpatients did not have a documented discussion of hospice with their care provider. Educating physicians to recognize the stepwise decline of most illnesses and hospice admission criteria will facilitate a more informed decision‐making process for patients and their families. A consistent commitment to offer hospice earlier than the terminal admission would increase access to community or home‐based care, potentially increasing quality of life. Journal of Hospital Medicine 2011;. © 2011 Society of Hospital Medicine.
Time‐resolved fluorescence microscopy of hematoporphyrin‐derivative in cells
Authors: Docchio F. , Ramponi R. , Sacchi C.A. , Bottiroli G. , Freitas I. 
Published: November 2011

AbstractThis work presents measurements of time‐resolved fluorescence microscopy of hematoporphyrin‐derivative (HpD) in single cells of mice tissue (both tumor and normal cells), in HeLa cells, and in solution. The measurements were performed using a pulsed‐laser microfluorometer with high spatial and temporal resolution. In agreement with the results obtained with other techniques, it has been found that the tumor cells examined present an HpD uptake about five times higher than that of the normal cells of the corresponding tissue and that, within a cell, HpD becomes localized mainly in the cytoplasm. It has also been found that the fluorescence decay time is different in cells as compared with solution, and that the presence of HpD stabilizes cell auto‐fluorescence. These results are discussed.
The plasma scalpel: A new thermal knife
Authors: Glover J. L. , Bendick P. J. , Link W. J. , Plunkett R. J. 
Published: November 2011

AbstractA new thermal knife, the plasma scalpel, capable of simultaneous division of tissue and coagulation of blood vessels, is described. A high temperature argon gas plasma (unrelated to blood plasma) is created by passing the gas through a direct current arc, ionizing the gas and elevating its temperature to 3000°C. A small plasma cutting jet is formed by a nozzle at the tip of the handpiece. Liver resections and muscle transections performed in a canine model, and full thickness burn excisions in a pig skin model showed effective division of tissue and significantly less blood loss when compared to the steel scalpel. Wound healing studies of histologic comparisons and wound breaking strength were performed for steel, plasma, laser, and electrosurgical scalpel incisions in the skin of mice and rats. All thermal knife wounds showed localized tissue damage at the edges of the incision, but the events of healing began at the same time. Compared to the steel scalpel, there was a three to six day delay in the onset of healing, but healing occurred in the normal fashion and all thermal knife wounds reached the same healed breaking strength as the steel scalpel wounds. Clinically, the plasma scalpel has been used for 138 procedures in 96 patients. The majority of cases have been transection of muscle, hepatic resection or debridement, or soft tissue debridement. Muscle transection data for the plasma scalpel compared to the electrosurgical scalpel has shown virtually no blood loss with a shorter time to hemostasis for the plasma scalpel. The plasma scalpel has proved to be an effective thermal knife, capable of simultaneous division and coagulation. Further development work and proof of its efficacy in the clinical setting are necessary to establish the plasma scalpel and adequately demonstrate its proper role in surgery.
Synthesis of Polyester Nanoparticles in Miniemulsion Obtained by Radical Ring‐Opening of BMDO and Their Potential as Biodegradable Drug Carriers
Authors: Siebert Joerg Max , Baumann Daniela , Zeller Anke , Mailänder Volker , Landfester Katharina 
Published: November 2011

Abstract5,6‐Benzo‐2‐methylene‐1,3‐dioxepane (BMDO) is used to obtain degradable polymeric nanoparticles via a statistical free‐radical copolymerization with MMA and styrene in direct miniemulsion. The nanoparticles are analyzed by means of IR, NMR, DLS, SEM, and TEM. They show excellent cellular uptake and drug delivery properties. The cellular uptake into HeLa cells of particles resulting from copolymerization of BMDO with styrene is drastically enhanced compared to pure polystyrene. As a model drug system, paclitaxel is incorporated in PBMDO particles and its release and the effect on HeLa cells is studied and compared to commercial drug formulations. It is found that a drug delivery system based on PBMDO shows an excellent pharmacological effect.magnified image
Development of a Biodegradable, Temperature‐sensitive Dextran‐based Polymer as a Cell‐detaching Substrate
Authors: Sun Guoming , Kusuma Sravanti , Gerecht Sharon 
Published: November 2011

AbstractA biodegradable, temperature‐sensitive dextran‐allyl isocyanate‐ethylamine (TSDAIE) as a nonenzymatic cell detachment polymeric substrate for human endothelial progenitor cells (EPCs) is developed and examined. The lower critical solution temperature of TSDAIE is determined; its phase transition occurrs at 18 to 22 °C. For EPC culture, cell culture flasks are coated with TSDAIE and type I collagen. The TSDAIE coating enables EPC detachment when the culture is cooled to 4 °C. The concentration of TSDAIE affects EPC attachment, which is thereby used to optimize the concentration of TSDAIE for coating. At the determined optimal concentration, TSDAIE is found to be compatible for use in EPC culture as revealed by cell attachment, spreading, proliferation, and phenotype. Overall, biodegradable TSDAIE shows promise for applications that culture and expand EPCs including vascular regenerative medicine and tissue engineering.magnified image
Algebraic semantics for the (↔, ¬¬)‐fragment of IPC
Authors: Słomczyńska Katarzyna 
Published: November 2011

AbstractWe show that the variety of equivalential algebras with regularization gives the algebraic semantics for the (↔, ¬¬)‐fragment of intuitionistic propositional logic. We also prove that this fragment is hereditarily structurally complete.
JmjC‐domain containing histone demethylase 1B‐mediated p15Ink4b suppression promotes the proliferation of leukemic progenitor cells through modulation of cell cycle progression in acute myeloid leukemia
Authors: Nakamura Satoki , Tan Lin , Nagata Yasuyuki , Takemura Tomonari , Asahina Aya , Yokota Daisuke , Yagyu Tomohiro , Shibata Kiyoshi , Fujisawa Shinya , Ohnishi Kazunori 
Published: November 2011

AbstractThe histone demethylase JHDM1B has been implicated in cell cycle regulation and tumorigenesis. In addition, it has been reported that JHDM1B is highly expressed in various human tumors, including leukemias. However, it is not clearly understood how JHDM1B contributes to acute myeloid leukemia (AML) cell proliferation. In this study, we investigated the cellular and molecular function of JHDM1B in AML cells. In AML cell lines and AML‐derived ALDHhi (high aldehyde dehydrogenase activity)/CD34+ cells, the levels of JHDM1B mRNA were significantly higher than in normal ALDHhi/CD34+ cells. Reduction of JHDM1B expression in AML cells inhibited cell proliferation compared to control cells, through induction of G1 cell cycle arrest, an increase in the p15Ink4b mRNA and protein expression. JHDM1B mRNA was overexpressed in all 133 AML clinical specimens tested (n = 22, 57, 34, and 20 for M1, 2, 4, and 5 subtypes respectively). Compared to normal ALDHhi/CD34+ cells, JHDM1B gene expression was 1.57‐ to 1.87‐fold higher in AML‐derived ALDHhi/CD34+cells. Moreover, the JHDM1B protein was more strongly expressed in AML‐derived ALDHhi/CD34+ cells from compared to normal ALDHhi/CD34+ cells. In addition, depletion of JHDM1B reduced colony formation of AML‐derived ALDHhi/CD34+ cells due to induction of p15Ink4b expression through direct binding to p15Ink4b promoter and loss of demethylation of H3K36me2. In summary, we found that JHDM1B mRNA is predominantly expressed in AML‐derived ALDHhi/CD34+ cells, and that aberrant expression of JHDM1B induces AML cell proliferation through modulation of cell cycle progression. Thus, inhibition of JHDM1B expression represents an attractive target for AML therapy. © 2011 Wiley Periodicals, Inc.
Oligomycin A enhances apoptotic effect of TRAIL through CHOP‐mediated death receptor 5 expression
Authors: He Long , Jang Jae Hyuk , Choi Hyun Gil , Lee Sun Mi , Nan Mei Hua , Jeong Sook Jung , Dong Zigang , Kwon Yong Tae , Lee Kyung Sang , Lee Ki Won , Chung Jong Kyeong , Ahn Jong Seog , Kim Bo Yeon 
Published: November 2011

AbstractDevelopment of resistance to TNF‐related apoptosis‐inducing ligand (TRAIL) in tumor cells is one of the important problems in cancer treatment. Despite the previous report demonstrating that oligomycin suppressed TNF‐induced apoptosis, in our screening of small molecules enhancing cancer cell death to TRAIL, oligomycin A (OMA) was found to enhance TRAIL‐induced apoptosis in HeLa cells. CCAAT/enhancer‐binding protein homologous protein (CHOP) was found to directly bind to death receptor 5 (DR5) promoter through endoplasmic reticulum stress (ER‐stress) signaling and sensitize the cells to TRAIL. Among ER‐stress associated proteins, OMA triggered the inositol‐requiring enzyme 1 (IRE1) signaling pathway, leading to X‐binding protein 1 (XBP1) splicing, CHOP expression and DR5 upregulation. In contrast, small‐interfering RNA (siRNA) of CHOP reduced the number of apoptotic cells in response to the co‐treatment of TRAIL and OMA. Collectively, our data suggest that OMA enhances apoptotic death of cervical cancer cells to TRAIL through upregulation of CHOP‐mediated DR5 expression following ER‐stress. © 2011 Wiley Periodicals, Inc.
Androgen receptor signals regulate UDP‐glucuronosyltransferases in the urinary bladder: A potential mechanism of androgen‐induced bladder carcinogenesis
Authors: Izumi Koji , Zheng Yichun , Hsu Jong‐Wei , Chang Chawnshang , Miyamoto Hiroshi 
Published: November 2011

AbstractUDP‐glucuronosyltransferases (UGTs), major phase II drug metabolism enzymes, play an important role in urinary bladder cancer initiation by detoxifying carcinogens. We aimed to determine if androgens regulate UGT expression via the androgen receptor (AR) pathway in the bladder. Real‐time reverse transcription‐polymerase chain reaction and Western blot analyses were used to assess UGT1A levels in the normal urothelium SVHUC cell line stably expressed with AR and in bladder tissues from AR knockout (ARKO) and castrated male mice. Immunohistochemistry was also performed in radical cystectomy specimens. Dihydrotestosterone (DHT) treatment in SVHUC‐AR reduced mRNA expression of all the UGT1A subtypes (19–75% decrease), and hydroxyflutamide antagonized the DHT effects. In contrast, DHT showed only marginal effects on UGT1A expression in SVHUC‐Vector. Of note were higher expression levels of UGT1As in SVHUC‐Vector than in SVHUC‐AR. In ARKO mice, all the Ugt1a subtypes were up‐regulated, compared to wild‐type littermates. In wild‐type male mice, castration increased the expression of Ugt1a8, Ugt1a9, and Ugt1a10. Additionally, wild‐type female mice had higher levels of Ugt1a than wild‐type males. Immunohistochemical studies showed strong (3+) UGT1A staining in 11/24 (46%) cancer tissues, which was significantly lower than in corresponding benign tissues [17/18 (94%) cases (P = 0.0009)]. These results suggest that androgen‐mediated AR signals promote bladder carcinogenesis by down‐regulating the expression of UGTs in the bladder. © 2011 Wiley Periodicals, Inc.