bluuebunny/crossref_metadata_2025_split huggingface dataset:
P42 Cutaneous reactions from non‐steroidal anti‐inflammatory drugs: follow‐up of 4 years
Authors: Galindo Esther Cuerda , Buján J Goday , Jorge B Fernández , Lozano J Rodríguez , Fonseca E 
Published: August 2010

Introduction:  Cutaneous reactions due to use of non‐steroidal anti‐inflammatory drugs (NSAID) are frequently described. In the Contact Unit from the Department of Dermatology of Juan Canalejo Hospital, we compiled all cases studied during 4 years (from 2000 to 2003) to analyze the incidence, frequency and type of reactions derived from NSAIDs use.Material and methods:  We used a NAIDs battery (Aristegui lab.Ò) on 55 patients with clinical suspicion of adverse reaction from NSAIDs. We performed epicutaneous study on 17 and additional photoallergic study, after UVA irradiation (from 5 to 7,5 J/cm2), on 38 cases. In some cases we patched the drug used by patient as is and/or the active substance provided by the laboratory if it was not included on NSAIDs battery. Readings were done following ICDRG criteria at 48 and 96 hours on patch tests and 24 and 72 hours on photopatch tests. Healthy controls were patched.Results:  22 patients showed positive test: 15 cases were diagnosed as photoallergic dermatitis (etofenamate 5; dexketoprofen 4; ketoprofen 3; piroxicam 2; diclophenac 2 and aceclophenac 1). Five cases were explained as allergic contact dermatitis (etofenamate 2; fepradinol 2 and phenylbutazone 1) and two cases of fixed drug eruption from piroxicam were found.Conclusion:  In this study we observed that cutaneous reactions due to ketoprofen and piroxicam are still high. It is important to note the high incidence of allergic and photoallergic reactions from dexketoprofen, a recently introduced NSAID, reporting always a cross reaction between ketoprofen and dexketoprofen.
P55 Nordic Occupational Skin Questionnaire (NOSQ‐2002)
Authors: Flyvholm Mari‐Ann , Susitaival P , Meding B , Kanerva L , Lindberg M , Svensson Å , Ólafsson JH 
Published: August 2010

Questionnaire‐tools for surveying occupational skin diseases and exposure are needed for comparable epidemiological research, workplace assessments, and evaluation of workplace interventions. The Nordic Occupational Skin Questionnaire Group supported by the Nordic Council of Ministers has developed a standardized questionnaire‐tool for surveys on work‐related skin diseases and skin exposures to environmental factors.Nordic Occupational Skin Questionnaire (NOSQ‐2002) includes two questionnaires designed for separate purposes. NOSQ‐2002/short is a 4‐page questionnaire for screening skin problems at workplaces. NOSQ‐2002/long is an in‐depth survey tool for research purposes. The questionnaire covers occupational history, atopic symptoms, self‐reported hand or forearm eczema, exacerbating factors, consequences and life impact of dermatoses, self‐reported contact urticaria on hands or forearms, skin symptoms, skin tests, exposures, and protective glove use. For the time being, NOSQ‐2002 is available in English, Danish, Swedish, Finnish and Icelandic. Further translations are welcomed.The NOSQ‐2002 report includes a review of pertinent literature on questionnaire methods for skin disease studies. Questions on work and exposure can be tailored to specific populations or occupational groups, according to the instructions and recommendations given in NOSQ‐2002/INFO version of the questionnaire.The NOSQ‐2002 questionnaire files can be downloaded from http://www.ami.dk/NOSQ. The Nordic Council of Ministers has the copyright to the NOSQ‐2002 questionnaires. Use of the questionnaires is free of charge. The NOSQ‐2002 questionnaires and their present and possible future translations cannot be used commercially.
P67 Hand eczema, skin exposure and glove use in dental technicians
Authors: Meding Birgitta , Hosseiny S , Wrangsjö K , Andersson E , Hagberg S , Wass K , Torén K , Brisman J 
Published: August 2010

Aims:  To estimate the incidence of hand eczema in dental technicians and to elucidate occupational risk factors.Methods:  This was a retrospective cohort study among former dental technician students (n = 2139). Controls comprised population controls (n = 2288) with similar distribution of age and sex as the dental technicians. A mail questionnaire asked for occurrence of hand eczema including age of onset, occupational exposure and use of protective gloves. The response rate was 58%(1210 dental technicians and 1316 controls answered the questionnaire).Results:  In dental technicians the incidence of hand eczema was 7.1 cases/1000 person years in males and 10.8 in females during acrylate‐exposed time. In controls the incidence of hand eczema was 3.1 in males and 3.7 in females. The relative risk (RR) for males was 2.3 (95% CI 1.6–3.4) and for females 2.9 (95% CI 2.1–4.0). 48% of the dental technicians and 30% of the controls reported more than 10 hand‐washings/day (p < 0.001). 80% of the dental technicians reported skin exposure to uncured acrylates, 14% daily. 87% had skin contact with grinding dust from acrylates, 42% daily. 38% used protective gloves when handling uncured acrylates, males 29%, females 48%(p < 0.001). 58% did not know for how long the normally used gloves protected the skin against uncured acrylates.Conclusions:  The risk of hand eczema is more than doubled in dental technicians. The work involves frequent and unprotected exposure to acrylates and wet work. Education regarding skin protection is important.
FS01.1 The use of azo dyes in the European Union – legislation review
Authors: Azenha Angelo 
Published: August 2010

The aim of this paper is to review the European legislation concerning the use of textile dyes.It is known that different dyes of different classes can be sensitizing and cause allergic contact dermatitis for both textile workers and consumers.The European Union has created restraining directives regarding the manufacture and distribution of potentially dangerous products, such as aromatic amines. A specific scientific committee of EU stated that the carcinogenic capacity of some azo dyes is related to chemical releasing of aromatic amines (groups MAK III; A1; A2).In this paper, focus will be centred on the latest European Union Directives in this matter and the prohibition of some azo dyes in textile industry.
FS01.3 Disperse (yes), orange (yes), 3 (no): what do we test in textile dye dermatitis?
Authors: Le Coz Christophe J , Jelen G , Goossens A , Vigan M , Ducombs G , Bircher A , Giordano‐Labadie F , Pons‐Guiraud A , Milpied‐Homsi  , Castelain M , Tennstedt D , Bourrain J‐L , Bernard G 
Published: August 2010

Introduction:  Patients sensitized to para‐phenylenediamine (PPD) have a high degree of patch test reactivity to Disperse Orange 3 (DO3), and a lesser one to Disperse Red 1 and Red 17. Two successive patients positive to PPD, Disperse Red 1 and 17, negative to DO3 were real eye‐openers for our considerations about purity of our current allergen DO3.Materials and methods:  We realized comparative thin‐layer chromatography (TLC), with DO3 from Chemotechnique®(DO3‐Chem) and Trolab®(both extracted from petrolatum), and “pure” DO3 from two chemical providers. TLC clearly indicated that DO3‐Chem was not DO3. HPLC analysis with pure DO3 from Chemotechnique® and comparison of structures by NMR with samples of DO3, revealed that DO3‐Chem was Disperse Orange 31 (DO31). In addition, signals through the GERDA network allowed the collection of test materials and observations. Among other members, only 2 used DO3‐Chem (from 2 different batches) that was DO31 too, according to TLC Results: According to their data, they observed no or a lower reactivity to DO3 than expected (4 patients DO3‐Chem + among 23 PPD+ e.g.). Finally, the error was proved to be due to the provider of the dye to Chemotechnique®, who likely deleted the 1 of Disperse Orange 31 on his packaging.Discussion:  Chemical structure of DO31 indicates a possible in vivo hydrolysis into nitroaniline and a second compound, a substituted PPD derivative that clearly does not frequently react in PPD positive patients. Like drugs, patch tests are submitted to post‐commercialization controls. In addition to allergens providers who should enhance their quality controls, dermato‐allergologists have to be vigilant, and must active networks when they observe a rare bird.
FS01.8 Substance‐specific modulation of TNF‐Alpha and IL‐1Beta in human monocytes by para‐Phenylenediamine and not by its autoxidation product Bandrowski’s Base
Authors: Hamidizadeh Nasrin , Breuer S , Merk HF , Bloemeke B 
Published: August 2010

Para‐phenylenediamine (PPD), an arylamine dye, is a strong allergen causing allergic contact dermatitis. Cytokines such as TNF‐α and IL‐1beta are key mediators in the initiation of this reaction. Both cytokines are predominantly produced by stimulated monocytes and macroghages. We investigated the responses of PPD and Bandrowski's base (BB), an autoxidation product of PPD in human monocytes. We isolated monocytes from healthy volunteers and incubated them with the allergens. TNF‐α and IL‐1beta mRNA expression and protein levels were estimated after 45 min, 2 h, 4 h and 24 h after allergen contact. IL‐1beta and TNF‐alpha were measured in cell culture supernatants by ELISA (n = 7) and mRNA expression was determined by real‐time RT‐PCR. We found that PPD reduced TNF‐α protein secretion by 20–69.9%(n = 6). Further, IL‐1beta levels were decreased by 44–98%. The same tendency was found studying IL‐1beta and TNF‐α mRNA steady state levels (n = 3; 1 h incubation). These effects were substance‐specific and not found for PPD derivatives nor for the autoxidation product BB. These findings suggest that PPD may specifically modify immune responses by directly infering with the cellular proinflammatory cytokine network.This study was supported by the EU.
FS02.7 Rates of persistent itchy nodules after fourth dose of DT vaccines
Authors: Netterlid Eva , Bruze M , Hindsén M , Isaksson M , Olin P 
Published: August 2010

Background:  Studies in Gothenburg, Sweden, reported unexpectedly high rates, 0.35–1.66%, of late occurring itching nodules at the site of injection of aluminium containing vaccines. Among 455 children with itching nodules after vaccination, 352 had a positive epicutaneous test to aluminium. When switching to a new booster diphtheria‐tetanus vaccine in Sweden these findings warranted a comparative study of local itching nodules that had remained at least 2 months after injection, and of aluminium contact allergy.Methods:  A prospective cluster randomised study was done in 25,232 10‐year‐olds. Participating schools in each municipality were randomised in a 1/1 ratio to use the old diphtheria‐tetanus toxoid (DT) vaccines, Duplex® or the new vaccine, diTeBooster®. Parental reports 6 months after vaccination were obtained for 22,365 (88%) pupils in 851 schools. Patch testing with aluminium chloride‐hexahydrate was performed in 9 children with an itching nodule, 6 children only pruritus and 17 children without any itching nodule after the fourth dose of DT vaccine.Results:  We identified 3–6 children per 10000 with a local itching nodule persisting for at least 2 months. There were no significant differences between the vaccine groups. Contact allergy to aluminium was not detected.Conclusion:  Our findings support the use of the vaccine presently available in the Swedish vaccination program. Continued surveillance of persistent itching nodules after vaccination with different aluminium containing DT and diphtheria‐tetanus‐pertussis toxoid (DTP) vaccines is however warranted.
Adenosine Receptor A2a is Differentially Expressed in CD4+ T Lymphocytes of Asthmatic and Healthy Individuals
Authors: Holopainen P. , Karagiannidis C. , Rückert B. , Hense G. , Schmidt‐Weber C. , Blaser K. 
Published: August 2010

The endogenous nucleoside adenosine is released in excess during inflammation or other metabolic stress and is generally known to deliver tissue protective anti‐inflammatory effects. Adenosine acts via four adenosine receptors of which the A2a receptor is the predominant form in T cells. Adenosine levels are elevated in asthmatic lung, and adenosine can directly induce mast cell degranulation and bronchoconstriction in these patients. Instead, the role of anti‐inflammatory mechanisms of adenosine on T cells in asthma is unclear.Aim:  To study the A2a receptor expression in peripheral blood CD4+ T cells in asthmatic and healthy individuals using flow cytometric and quantitative real‐time PCR methods.Results:  Unstimulated CD4+ cells of asthmatic patients expressed significantly lower levels (P < 0.001) of A2a receptor in protein level (mean percentage of cells positive ± SEM: 76.8 ± 1.2, n = 6) compared to healthy individuals (90.4% ± 1.9, n = 4). Double staining for CD69 expression showed that stimulation of CD4+ cells decreased A2a expression in both groups but indicated that the detected lower levels of A2a in unstimulated cells of asthmatics was not due to preactivation in these patients. Surprisingly, A2a mRNA expression in unstimulated CD4+ cells was significantly higher (P < 0.05) in asthmatics (n = 28) compared to healthy controls (n = 7). The expression did not correlate with serum total IgE levels.Conclusions:  Asthmatic individuals express less A2a adenosine receptor on their peripheral CD4+ T cells. The higher mRNA levels instead may point to a negative feedback regulation in the receptor expression. The role of possibly decreased adenosine‐mediated anti‐inflammatory effects in asthma pathogenesis require further studies on this T‐cell mediated disease.
Expression of Human Collectins in Colorectal Carcinoma
Authors: Siassi M. , Hohenberger W. , Croner R. 
Published: August 2010

Introduction:  The human collectins, mannan‐binding lectin (MBL), surfactant protein‐A (SP‐A) and surfactant‐protein‐D (SP‐D) play a central role in the innate immune system. Immunological responses to malignant transformation of epithelial cells gained increasing interest recently. A former study could demonstrate binding of MBL to certain colorectal carcinoma (CRC) cell lines in vitro. We therefore examined the expression of human collectins in normal colon mucosa and in colorectal carcinomas.Materials and methods:  Colon samples from 20 CRC patients and 10 normal mucosa samples were collected immediately after surgery. The tissue was microdissected and RNA isolated (Qiagen, Rneasy‐Kit). Gene expression profiles were analysed using Gene‐chips (Affymetrix, HG‐U133). We analysed the data for the expression of MBL, its associated serine proteases mannan‐binding lectin‐associated serine protease 1/2 (MASP 1/2), SP‐A and SP‐D. The signal intensity of the genes of interest was compared using the Mann–Whitney U‐test.Results:  The expression of human collectins in normal human colon mucosa was generally low. Only the expression of SP‐A and MASP‐2 reached the noise threshold of 250 signals. These genes were significantly downregulated in CRC specimens. The expression of the other proteins showed no difference in normal mucosa and CRC.Conclusion:  As demonstrated before, the expression of human collectins in normal colon was low in this study. Only SP‐A showed a significant expression in normal mucosa which was downregulated in CRC. As the absolute signal level was below the noise threshold, these results have to be interpreted with caution and require confirmation by direct measurenment of the proteins. Our results suggest that there is no major role for the human collectins in colorectal cancer.
Acute Effects of Barbiturates in Parkinson's Disease
Authors: Sturfelt Gunnar , Schelin Sonny 
Published: August 2010

Parkinsonism has by tradition been treated with sedatives, e.g. barbiturates. The intention has then been to reduce the stress‐related component in extrapyramidal symptoms. On the basis of our own clinical observations we have proposed an effect of barbiturates that seems to be specific pharmacologically.We have noticed that pentymal sodium has visibly improved the symptoms of morbus Parkinson (MP). Our first observation of this effect was made at the Psychiatric Department when narco‐analysing a patient with psychoneurosis also suffering from MP. We could reproduce the same effect in other patients with this disease without any signs of psychoneurosis. On the basis of this observation we have made a minor pilot study.Pentymal sodium has been administered slowly i.v. in doses of 0.2‐0.3 g according to body weight. During the initial hypnotic phase a moderate reduction of the tremor seems to be correlated to the sedative effect. After 1–2 hours when the patients are less sedated, a reduction of tremor, brady‐kinesia and rigidity appears. The effect has usually lasted for 12–15 hours. The symptoms have been estimated according to Webster's score (4) before and after the administration.
Ergonovine Testing in Patients with Exertional Angina
Authors: NORDLANDER ROLF , ORINIUS ERIK 
Published: August 2010

ABSTRACT. Nordlander R, Orinius E (Division of Cardiology, Department of Internal Medicine, Thoracic Clinics, Karolinska Hospital, Stockholm, Sweden). Ergonovine testing in patients with exertional angina. Acta Med Scand 1987; 221:47–52.Injection of ergonovine has been suggested as a diagnostic test in patients with suspicion of a vasospastic component in the pathophysiology of angina pectoris. However, a thorough case history has been considered by others to give the same information regarding the anginal mechanism. Therefore a bedside ergonovine test (0.075–0.675 mg i.v.) was performed in 21 consecutive patients with effort angina in order to study the relation between the outcome of the test and the case history concerning angina at rest. A coronary angiography was performed in all cases and showed significant stenoses in 17 patients and normal coronary arteries in 4. Eight patients had angina only during effort, 11 had angina both during effort and at rest and 2 patients had atypical chest pain. Ten of the 11 patients with concomitant rest angina developed chest pain at the ergonovine test and 9 coexisting ECG changes. However, these effects were about as common among the 8 patients without angina at rest: 7 developed chest pain and 4 also ECG changes. The two patients with atypical chest pain had normal coronary angiograms and in these patients ergonovine provoked the same atypical chest pain but no ECG changes. Thus, a concomitant angina at rest is a common finding among patients with severe effort angina. However, the response to ergonovine was as common in the group with only effort angina as in the group with concomitant rest angina, indicating the limited value of this test in patients with severe angina.
The Anatomy of the Head of the New Zealand Frog, Leiopelma
Authors: Stephenson Elsie M. 
Published: August 2010

Summary.An attempt has been made to present a full account of the anatomy of the head (exclusive of the inner ear) of Leiopelma. Material from two species, Leiopelma archeyi and Leiopelma hochstetteri, has been studied and the head of the latter is here described for the first time.As far as possible, comparison has been made with the head of Ascaphus, the only other genus included in the same primitive anuran family.Specific differences between Leiopelma archeyi and Leiopelma hochstetteri are described and listed.A possible homology is suggested between the processus lingularis of Anura and Seydel's palatal process of Urodela.Particular emphasis is placed on the controversial topic of the basal connection between the quadrate and the neurocranium. An historical account of previous ideas on this subject is included, followed by a discussion based on the view that the ‘basal’ or ‘pseudobasal’ process of Leiopelma, together with the ledge of cartilage forming the floor of the hyomandibular canal, are of hyoid origin.
The growth‐stages of the pouch‐young of the Native Cat (Dasyurus viverrinus) together with observations on the anatomy of the new‐born young.*
Authors: Hill J. P. , Hill W. C. Osman 
Published: August 2010

Summary.The biology of the pouch‐young (mammary foetus) is considered in a graded series of stages of Dasyurus commencing with the new‐born, still unattached young and ending with the fully developed animal at the time it leaves the maternal pouch (at the age of 4 to 4 months) and capable of fending for itself. The series has been divided for convenience into seventeen stages, labelled A‐P, and the growth, external appearances and eruption of teeth has been fully considered.The problem of the migration of new‐born young to the pouch, their attachment to the nipples, and the mode of lactation and its duration have been considered, together with some comment of the supposed mammary compressor action of the cremaster muscles.The internal structure of the new‐born Dasyurus has been described in detail, special emphasis being laid on the precocious development of certain structures which are considered necessary adaptational machinery for the insurance of survival of the young in its unusual environment and more particularly those features required for the sole purpose of ensuring its safe arrival in the pouch, its becoming attached to a nipple and the certitude of its receipt of milk without hindrance to its respiration.Notable at birth are (i) the relatively advanced state of the fore‐limbs, (ii) the presence of deciduous claws upon the manual digits and their later replacement by definitive claws, (iii) the presence of the oral shield as a specialization upon the muzzle, (iv) the unique cervical swelling, (v) special features of the tongue and larynx, with the intranasal epiglottis (vi) the reptilian state of the lungs, (vii) the advanced condition of the stomach and duodenum compared with the rest of the gut, (viii) the state of development of brain and sense organs, the olfactory parts being especially forward in development, together with those parts of the nervous system necessary for controlling the movements of the fore‐limb, sucking and respiratory movements, (ix) the indifferent state of the gonads.Throughout comparison has been made with the new‐born of other marsupials, so far as these are known, and features common to them all or peculiar to Dasyurus have been pointed out.
The larger arthrodiran fishes from the area of the Burrinjuck Dam, N.S.W.
Authors: WHITE ERROL I. 
Published: August 2010

In this paper are described the seven larger arthrodiran fishes from the Lower Devonian strata of the Burrinjuck area in New South Wales. The first is an interesting new palae‐acanthaspid, but the six other fishes are euarthrodires remarkable not only for individual peculiarities but for a mixture of characters usually attributed to two or more of the main lines of arthrodiran evolution. They may be considered as early essays in the development of arthrodires, but they also suggest that the main groups were not descended one from another, as for instance the brachythoracids are often said to have evolved from the arcto‐lepids, but from a more distant common ancestor.The development of the apron is demonstrated and the nature of the periophthalrnic capsules in relation to sclerotic plates is discussed.