bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synthesis and in vitro antimicrobial activity of new 3-(2-morpholinoquinolin-3-yl) substituted acrylonitrile and propanenitrile derivatives
Authors: Makawana Jigar , Patel Manish , Patel Ranjan 
Published: June 2011

AbstractA series of new 3-(2-morpholinoquinolin-3-yl)acrylonitrile derivatives (IVa-IVf) has been synthesised by the base-catalysed condensation reaction of 2-morpholinoqionoline-3-carboxaldehydes (IIa-IIc) and 2-cyanomethylbenzimidazoles (IIIa-IIIb). Subsequent regiospecific reduction of the C—C double bond in acrylonitrile moiety afforded 3-(2-morpholinoquinolin-3-yl)propanenitrile derivatives (Va-Vf). All the compounds synthesised were subjected to in vitro antimicrobial screening against some representatives of bacteria and fungi. The majority of the compounds were found to be active against Gram-positive bacteria Bacillus subtilis and Clostridium tetani as well as against the fungal pathogen Candida albicans.
Intercalation of basic amino acids into layered zirconium proline-N-methylphosphonate phosphate
Authors: Zeng Ren-Quan , Fu Xiang-Kai , Yang Xin-Bin 
Published: June 2011

AbstractIntercalation of basic amino acids into layered zirconium proline-N-methylphosphonate phosphate (α-ZPMP) was investigated at room temperature. Three kinds of host-guest compounds were prepared and characterised by elemental analysis, inductively coupled plasma analysis (ICP), Fourier transform infrared spectrum (FT-IR), Raman spectrum, X-ray powder diffraction (XRD) and thermoanalysis. The interaction of amino acid guests with P-OH of α-ZPMP host was documented by FT-IR and Raman spectra. In addition, the XRD patterns indicated that l-arginine or l-lysine were intercalated into the interlayer galleries of α-ZPMP host; the interlayer distances of the Larginine and l-lysine intercalation compounds were expanded from 1.520 nm to 2.218 nm and 2.207 nm, respectively. l-arginine and l-lysine would be arranged as a mono-molecule layer in different orientations. The interlayer distance of l-histidine (d = 1.522 nm) was similar to that of α-ZPMP host (d = 1.520 nm), l-histidine might be adsorbed on the outer surface of the α-ZPMP host. Thermoanalysis showed that the intercalated l-arginine and l-lysine were removed at 110–305°C or 150–250°C, respectively, the adsorbed l-histidine was released at a temperature of up to 320°C.
Analysis of streptolydigin degradation and conversion in cultural supernatants of Streptomyces lydicus AS 4.2501
Authors: Li Liangzhi , Fu Jiaolong , Qiu Yexian , Wang Jinlian 
Published: June 2011

AbstractA variety of structural analogues of streptolydigin exists in the cultural supernatants of Streptomyces lydicus AS 4.2501. The degradation of streptolydigin in cultural supernatants with different pH values kept at 25°C in a thermostatic bath was investigated using LC-MS/MS detection. Analysis of the alkaline supernatants (pH 9.50) provides evidence of degradation and conversion between streptolydigin and its structural analogues. Interestingly, a new streptolydigin analogue was detected by LC-MS and photo-diode array (PDA) detection in the process of alkaline degradation. After 48 h in a thermostatic bath, the degradation of streptolydigin and its two analogues at pH 9.50 approached pseudo-first order kinetics. Comparatively, the degradation of streptolydigin was much more rapid in the cultural supernatants with pH 3.05, only requiring 2 hours. Qualitative analysis of the degradation products by LC-MS/MS and PDA indicated that hydrolysis of the epoxy ether bond and acid amide bond was the major mechanism of degradation in acidic cultural supernatants. Two degradation products in the acid supernatant were assumed.
Terahertz detectors and focal plane arrays
Authors: Rogalski A. , Sizov F. 
Published: June 2011

AbstractTerahertz (THz) technology is one of emerging technologies that will change our life. A lot of attractive applications in security, medicine, biology, astronomy, and non-destructive materials testing have been demonstrated already. However, the realization of THz emitters and receivers is a challenge because the frequencies are too high for conventional electronics and the photon energies are too small for classical optics. As a result, THz radiation is resistant to the techniques commonly employed in these well established neighbouring bands.In the paper, issues associated with the development and exploitation of THz radiation detectors and focal plane arrays are discussed. Historical impressive progress in THz detector sensitivity in a period of more than half century is analyzed. More attention is put on the basic physical phenomena and the recent progress in both direct and heterodyne detectors. After short description of general classification of THz detectors, more details concern Schottky barrier diodes, pair braking detectors, hot electron mixers and field-effect transistor detectors, where links between THz devices and modern technologies such as micromachining are underlined. Also, the operational conditions of THz detectors and their upper performance limits are reviewed. Finally, recent advances in novel nanoelectronic materials and technologies are described. It is expected that applications of nanoscale materials and devices will open the door for further performance improvement in THz detectors.
Phase maps of polycrystalline human biological fluids networks: statistical, correlation, and fractal analysis
Authors: Ushenko Y. 
Published: June 2011

AbstractThe complex statistical and fractal analysis of phase properties, inherent to birefringence networks of liquid crystals consisting of optically-thin layers, prepared from synovial fluid taken from human joints, is performed in this work. Within the framework of a statistical approach, the authors have investigated values and ranges for changes of statistical moments of the 1-st to the 4-th orders that characterize coordinate distributions for phase shifts between orthogonal components of amplitudes inherent to laser radiation, transformed by synovial fluid layers, for human joints with various pathologies. The correlation criteria for differentiation of phase maps, describing pathologically changed liquid-crystal networks, have been ascertained. In the framework of the fractal approach, dimensions of self-similar coordinate phase distributions as well as features of transformation of logarithmic dependences for power spectra of these distributions for various types of human joint pathologies are determined.
Detection of brain micrometastases by high-resolution stereotactic magnetic resonance imaging and its impact on the timing of and risk for distant recurrences
Authors: Hanssens Patrick , Karlsson Bengt , Yeo Tseng Tsai , Chou Ning , Beute Guus 
Published: June 2011

Object
The aim of this study was to assess the order of micrometastases that can be detected with high-resolution MR imaging at the time of Gamma Knife surgery (GKS), and to estimate the impact this has on the time until and incidence of distant recurrences.

Methods
A consecutive series of 835 patients with brain metastases treated with GKS in a 7-year period, excluding patients in whom earlier brain metastases were treated with other modalities, were retrospectively analyzed. In all patients GKS was based on high Gd–dose (0.3 mmol/kg), high-resolution stereotactic MR imaging. These images were compared with the standard pretreatment MR images, and the difference in number of metastases found was analyzed. The distant recurrence rate following GKS was compared with that found in a prospective randomized study (Aoyama et al.) comparing radiosurgery to radiosurgery plus prophylactic whole-brain radiation therapy.

Results
New tumors were diagnosed in 40% (95% CI 36%–43%) of all patients as well as in the majority of patients with multiple lesions found on the diagnostic scan. The more tumors there were on the diagnostic scan, the higher the likelihood of detecting additional lesions with high-resolution imaging. It was calculated that approximately 50% of the micrometastases present at the time of GKS could be diagnosed with high-resolution imaging, which decreased the incidence of and delayed the time for the development of distant recurrences.

Conclusions
Additional brain metastases can be diagnosed in 40% of patients by using high-resolution imaging. Thus, radiosurgical treatments based on high-resolution stereotactic MR imaging decrease the incidence of and lengthen the time to distant recurrences.
Computed tomography–based morphometric analysis of the human occipital condyle for occipital condyle–cervical fusion
Authors: Le Tien V. , Dakwar Elias , Hann Shannon , Effio Euclides , Baaj Ali A. , Martinez Carlos , Vale Fernando L. , Uribe Juan S. 
Published: June 2011

Object
Occipital condyle screws serve as an alternative fixation point in occipital-cervical fusion. Their placement requires a thorough understanding of the anatomy of the occipital condyles and associated structures. This study is a CT-based morphometric analysis of occipital condyles as related to occipital condyle–cervical fusion.

Methods
A total of 170 patients were examined with CT scans of the craniocervical junction at a single institution, for a total of 340 occipital condyles, between March 6, 2006, and July 30, 2006. All CT scans were negative for traumatic, degenerative, and neoplastic pathological entities. Condylar anteroposterior (AP) length, transverse width, height, projected screw angle, and projected screw lengths were measured on an EBW Portal 2.5 CT Viewer Workstation (Philips Electronics).
The longest axis in the AP orientation of the occipital condyle was accepted as the length. The transverse width was a line perpendicular to the midpoint of the long axis. The height was measured in the coronal projection that had the thickest craniocaudal portion of the condyle. The screw trajectory started 5 mm lateral to the medial edge of the condyle and a line was directed anteromedially in the longest axis. The angle was measured relative to the sagittal midline. The screw length was measured from the outer cortex of the posterior wall to the outer cortex of the anterior wall.

Results
The mean ± SD values for occipital condyle measurements were as follows: AP length was 22.38 ± 2.19 mm (range 14.7–27.6 mm); width was 11.18 ± 1.44 mm (range 7.4–19.0 mm); height was 9.92 ± 1.30 mm (range 5.1–14.3 mm); screw angle was 20.30° ± 4.89° (range 8.0°–34.0°); and screw length was 20.30 ± 2.24 mm (range 13.0–27.6 mm).

Conclusions
These measurements correlate with previous cadaveric and radiographic studies of the occipital condyle, and emphasize the role of preoperative planning for the feasibility of placement of an occipital condyle screw.
The Potential Role of Interferon-regulatory Factor 7 Among Taiwanese Patients with Systemic Lupus Erythematosus
Authors: LIN LI-HSIN , LING PIN , LIU MING-FEI 
Published: June 2011

Objective.Type I interferons (IFN), especially IFN-α, have been proposed to underlie the pathogenesis of systemic lupus erythematosus (SLE). Members of the IFN regulatory factor (IRF) family, which regulate IFN expression, have been implicated as risk factors for SLE. Our aims were to investigate the expression of IRF7 and its correlation with disease activity and to explore the association in Taiwanese patients between 2 genetic single-nucleotide polymorphisms (SNP) of IRF7 and SLE.Methods.IRF7 messenger RNA (mRNA) levels were measured in peripheral blood mononuclear cells by real-time reverse transcription polymerase chain reaction in 51 adult patients with SLE and 65 age-matched and sex-matched controls. Their serum IFN-α levels were determined by ELISA and the clinical manifestations were recorded at the same time. Two IRF7 SNP, rs1061501 and rs1061502, were examined by genotyping across 92 patients with SLE and 92 age and sex-matched healthy control subjects.Results.Compared with controls, the expression of IRF7 mRNA was significantly increased in patients with SLE and was positively correlated with both the serum level of IFN-α and lupus disease activity. The distribution of SNP rs1061501 by genotype (CC, CT, and TT) and by allele (C, T) was significantly different between the SLE and the control group (p = 0.028 for genotype and p = 0.009 for allele). There were no significant differences for SNP rs1061502.Conclusion.The results suggest that dysregulation of IRF7 might mediate an excessive production of IFN-α, which then exerts a crucial effect on the pathogenesis of human SLE. The IRF7 SNP rs1061501 TT genotype and T allele are enriched in Taiwanese patients with SLE and thus would seem to be associated with an increased risk of developing SLE.
Effectiveness of Interference Screw Fixation in Ulnar Collateral Ligament Reconstruction
Authors: Nissen Carl W. 
Published: June 2011

Ulnar collateral ligament disruptions of the elbow are increasingly common for athletes involved in overhead sports. One newer reconstructive technique combines the use of a biotenodesis screw for ulnar fixation with the docking procedure for humeral fixation referred to as the DANE procedure. Biomechanical evaluations have determined that the combined procedure has properties similar to previous methods, but few comparative clinical studies have been performed. The current study compares the DANE procedure to a traditional method of ulnar collateral ligament reconstruction with a medium range follow-up.
Twelve ulnar collateral ligament reconstructions were observed for a minimum of 21 months. Six reconstructions were performed using bone tunnels on the ulna. The other 6 used a biotenodesis interference screw on the ulna. Both groups used the docking procedure on the humerus. Identical rehabilitation phases were followed by both groups. Attention was paid not only to the time to return to play and the level able to be returned to but also to interoperative differences, including tourniquet time.
We concluded that the DANE reconstruction is an appropriate and effective method. Results are similar to those achieved with a traditional reconstructive method. The study also demonstrates that tourniquet time and, therefore, operative time is less for the DANE procedure.
Acute Aortic Dissection or Ruptured Aortic Aneurysm Associated with Back Pain and Paraplegia
Authors: Yoshioka Katsuhito , Toribatake Yasumitsu , Kawahara Norio , Tomita Katsuro 
Published: June 2011

Acute aortic dissection and a ruptured aortic aneurysm are both catastrophic events that usually present suddenly. Although these conditions are relatively uncommon compared to other diseases treated by the orthopedists as a primary practitioner, they are of primary importance. The purpose of this study was to investigate the key points that differentiate these conditions from spinal disease.
A review of 50 patients with aortic dissection (n=40) or a ruptured aortic aneurysm (n=10) was performed to determine the manifestations (eg, back pain and paraplegia). We also evaluated the predisposing factor and blood pressure on admission and reviewed clinical imaging (radiographs, computed tomography [CT]) retrospectively.
Sudden severe isolated back pain was observed in 18 (45.0%) of 40 patients, and 31 (77.5%) of 40 patients had at least some back pain in aortic dissection, while 1 patient had sudden paraplegia with a ruptured aortic aneurysm. Hypertension was the most predisposing factor and was present in 29 (58.0%) of 50 patients. On admission, hypertension was present in 26 (56.5%) of 46 patients, and hypotension was present in 14 (30.4%) of 46 patients. In all cases, the correct diagnosis was made based on CT.
For a patient with an abrupt onset of severe back pain, acute aortic dissection and a ruptured aortic aneurysm should be considered in the differential diagnosis from spinal disease. The most reliable tool for imaging diagnosis was CT.
Peroxisome Proliferator-Activated Receptor γ-Regulated Cathepsin D Is Required for Lipid Antigen Presentation by Dendritic Cells
Authors: Nakken Britt , Varga Tamas , Szatmari Istvan , Szeles Lajos , Gyongyosi Adrienn , Illarionov Petr A. , Dezso Balazs , Gogolak Peter , Rajnavolgyi Eva , Nagy Laszlo 
Published: June 2011

Abstract
It is well established that dendritic cells (DCs) take up, process, and present lipid Ags in complex with CD1d molecules to invariant NKT cells. The lipid-activated transcription factor, peroxisome proliferator-activated receptor γ (PPARγ), has previously been shown to regulate CD1d expression in human monocyte-derived DCs, providing a link between lipid metabolism and lipid Ag presentation. We report that PPARγ regulates the expression of a lysosomal protease, cathepsin D (CatD), in human monocyte-derived DCs. Inhibition of CatD specifically reduced the expansion of invariant NKT cells and furthermore resulted in decreased maturation of saposins, a group of lipid transfer proteins required for lysosomal lipid Ag processing and loading. These results reveal a novel mechanism of lipid Ag presentation and identify CatD as a key component of this machinery and firmly place PPARγ as the transcriptional regulator linking lipid metabolism and lipid Ag processing.
Human Metapneumovirus Glycoprotein G Inhibits TLR4-Dependent Signaling in Monocyte-Derived Dendritic Cells
Authors: Kolli Deepthi , Bao Xiaoyong , Liu Tianshuang , Hong Chao , Wang Tian , Garofalo Roberto P. , Casola Antonella 
Published: June 2011

Abstract
Human metapneumovirus (hMPV) is a major cause of upper and lower respiratory infections in children and adults. Recent work from our group demonstrated that hMPV G glycoprotein is an important virulence factor, responsible for inhibiting innate immune responses in airway epithelial cells. Myeloid dendritic cells (DCs) are potent APCs and play a major role in initiating and modulating the innate and adaptive immune responses. In this study, we found that TLR4 plays a major role in hMPV-induced activation of monocyte-derived DCs (moDCs), as downregulation of its expression by small interfering RNA significantly blocked hMPV-induced chemokine and type I IFN expression. Similar results were found in bone marrow-derived DCs from TLR4-deficient mice. moDCs infected with a virus lacking G protein expression produced higher levels of cytokines and chemokines compared with cells infected with wild-type virus, suggesting that G protein plays an inhibitory role in viral-induced cellular responses. Specifically, G protein affects TLR4-dependent signaling, as infection of moDCs with recombinant hMPV lacking G protein inhibited LPS-induced production of cytokine and chemokines significantly less than did wild-type virus, and treatment of moDCs with purified G protein resulted in a similar inhibition of LPS-dependent signaling. Our results demonstrate that hMPV G protein plays an important role in inhibiting host innate immune responses, likely affecting adaptive responses too.
Migrational Guidance of Neutrophils Is Mechanotransduced via High-Affinity LFA-1 and Calcium Flux
Authors: Dixit Neha , Yamayoshi Itsukyo , Nazarian Ari , Simon Scott I. 
Published: June 2011

Abstract
Acute inflammation triggers the innate immune response of neutrophils that efficiently traffic from the bloodstream to concentrate at high numbers at the site of tissue infection or wounding. A gatekeeper in this process is activation of β2 integrins, which form bond clusters with ICAM-1 on the endothelial surface. These bond clusters serve dual functions of providing adhesive strength to anchor neutrophils under the shear forces of blood flow and directional guidance for cell polarization and subsequent transmigration on inflamed endothelium. We hypothesized that shear forces transmitted through high-affinity LFA-1 facilitates the cooperation with the calcium release-activated channel Orai1 in directing localized cytoskeletal activation and directed migration. By using vascular mimetic microfluidic channels, we observed neutrophil arrest on a substrate of either ICAM-1 or allosteric Abs that stabilize a high- or low-affinity conformation of LFA-1. Neutrophils captured via low-affinity LFA-1 did not exhibit intracellular calcium flux, F-actin polymerization, cell polarization, or directional migration under shear flow. In contrast, high-affinity LFA-1 provided orientation along a uropod–pseudopod axis that required calcium flux through Orai1. We demonstrate how the shear stress of blood flow can transduce distinct outside–in signals at focal sites of high-affinity LFA-1 that provide contact-mediated guidance for neutrophil emigration.
Sleep after Vaccination Boosts Immunological Memory
Authors: Lange Tanja , Dimitrov Stoyan , Bollinger Thomas , Diekelmann Susanne , Born Jan 
Published: June 2011

Abstract
Sleep regulates immune functions. We asked whether sleep can influence immunological memory formation. Twenty-seven healthy men were vaccinated against hepatitis A three times, at weeks 0, 8, and 16 with conditions of sleep versus wakefulness in the following night. Sleep was recorded polysomnographically, and hormone levels were assessed throughout the night. Vaccination-induced Th cell and Ab responses were repeatedly monitored for 1 y. Compared with the wake condition, sleep after vaccination doubled the frequency of Ag-specific Th cells and increased the fraction of Th1 cytokine-producing cells in this population. Moreover, sleep markedly increased Ag-specific IgG1. The effects were followed up for 1 y and were associated with high sleep slow-wave activity during the postvaccination night as well as with accompanying levels of immunoregulatory hormones (i.e., increased growth hormone and prolactin but decreased cortisol release). Our findings provide novel evidence that sleep promotes human Th1 immune responses, implicating a critical role for slow-wave sleep in this process. The proinflammatory milieu induced during this sleep stage apparently acts as adjuvant that facilitates the transfer of antigenic information from APCs to Ag-specific Th cells. Like the nervous system, the immune system takes advantage of the offline conditions during sleep to foster adaptive immune responses resulting in improved immunological memory.
Salmonella Synthesizing 1-Monophosphorylated Lipopolysaccharide Exhibits Low Endotoxic Activity while Retaining Its Immunogenicity
Authors: Kong Qingke , Six David A. , Roland Kenneth L. , Liu Qing , Gu Lillian , Reynolds C. Michael , Wang Xiaoyuan , Raetz Christian R. H. , Curtiss Roy 
Published: June 2011

Abstract
The development of safe live, attenuated Salmonella vaccines may be facilitated by detoxification of its LPS. Recent characterization of the lipid A 1-phosphatase, LpxE, from Francisella tularensis allowed us to construct recombinant, plasmid-free strains of Salmonella that produce predominantly 1-dephosphorylated lipid A, similar to the adjuvant approved for human use. Complete lipid A 1-dephosphorylation was also confirmed under low pH, low Mg2+ culture conditions, which induce lipid A modifications. lpxE expression in Salmonella reduced its virulence in mice by five orders of magnitude. Moreover, mice inoculated with these detoxified strains were protected against wild-type challenge. Candidate Salmonella vaccine strains synthesizing pneumococcal surface protein A (PspA) were also confirmed to possess nearly complete lipid A 1-dephosphorylation. After inoculation by the LpxE/PspA strains, mice produced robust levels of anti-PspA Abs and showed significantly improved survival against challenge with wild-type Streptococcus pneumoniae WU2 compared with vector-only–immunized mice, validating Salmonella synthesizing 1-dephosphorylated lipid A as an Ag-delivery system.