bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nicotine Enhances the Antiapoptotic Function of Mcl-1 through Phosphorylation
Authors: Zhao Jinfeng , Xin Meiguo , Wang Ton , Zhang Yangde , Deng Xingming 
Published: November 2009

Abstract
Lung cancer has a strong etiologic association with cigarette smoking. Nicotine, a major component in tobacco smoke, functions as a survival agonist that inhibits apoptosis following various stresses. However, the mechanism of action remains elusive. Mcl-1, a major antiapoptotic protein of the Bcl2 family, is extensively expressed in both small cell and non–small cell lung cancer cells, suggesting that Mcl-1 may be a therapeutic target of patients with lung cancer. Here, we found that nicotine induces Mcl-1 phosphorylation through activation of extracellular signal-regulated kinase 1/2 in association with increased chemoresistance of human lung cancer cells. Since nicotine stimulates Mcl-1 phosphorylation and survival in cells expressing wild-type but has no such effects in cells expressing T163A Mcl-1 mutant, this indicates that nicotine induces Mcl-1 phosphorylation exclusively at the T163 site and that phosphorylation of Mcl-1 at T163 is required for nicotine-induced survival. Mechanistically, nicotine-induced Mcl-1 phosphorylation significantly enhances the half-life of Mcl-1, which renders Mcl-1 a long-term survival activity. Specific depletion of Mcl-1 by RNA interference blocks nicotine-stimulated survival and enhances apoptotic cell death. Thus, nicotine-enhanced survival of lung cancer cells may occur through activation of Mcl-1 by phosphorylation at T163 site, which may contribute to development of human lung cancer and/or chemoresistance. (Mol Cancer Res 2009;7(12):1954–61)
The Association and Nuclear Translocation of the PIAS3-STAT3 Complex Is Ligand and Time Dependent
Authors: Dabir Snehal , Kluge Amy , Dowlati Afshin 
Published: November 2009

Abstract
The epidermal growth factor (EGF) receptor activation of downstream signal transducers and activators of transcription 3 (STAT3) plays a crucial role in the pathogenesis of lung cancer. STAT3 transcriptional activity can be negatively regulated by protein inhibitor of activated STAT3 (PIAS3). We investigated the time-dependent PIAS3 shuffling and binding to STAT3 in an EGF-dependent model in lung cancer by using confocal microscopy, immunoprecipitation, luciferase reporter assay, and protein analysis of segregated cellular components. We also explored the role of phosphorylation at Tyr705 of STAT3 in the formation and intracellular shuffling of the PIAS3-STAT3 complex. In a growth factor–free state, PIAS3 was localized to the cytoplasm and unbound to STAT3 in both H520 and A549 cells. On exposure to EGF, we observed STAT3 phosphorylation and rapid formation of the PIAS3-STAT3 complex. Within 5 minutes, there was a progressive translocation of the complex to the nucleus, and by 10 minutes, PIAS3 was uniquely localized to the nuclear compartment. After 30 minutes, PIAS3 returned to the cytoplasm. Using site-directed mutagenesis, we substituted Tyr705 of STAT3 with a phenylalanine. Despite EGF stimulation, we observed a significant decrease in PIAS3-STAT3 binding and a significant reduction in nuclear translocation of PIAS3. Furthermore, there was a significant reduction in the capacity of PIAS3 to reduce STAT3-mediated gene transcription. In wild-type STAT3 cells, increasing concentrations of PIAS3 resulted in a proportional decrease in STAT3 phosphorylation. These data suggest an important role for the negative regulatory effect of PIAS3 on STAT3 in EGF-driven tumors. (Mol Cancer Res 2009;7(11):1854–60)
Analysis of the Relationship between Banded Orographic Convection and Atmospheric Properties Using Factorial Discriminant Analysis and Neural Networks
Authors: Godart A. , Leblois E. , Anquetin S. , Freychet N. 
Published: November 2009

AbstractThe relationship between banded orographic convection and atmospheric properties is investigated for a region in the south of France where the associated rainfall events are thought to represent a significant portion of the hydrologic input. The purpose is to develop a method capable of producing an extensive database of banded orographic convection rainfall events from atmospheric sounding data for this region where insufficient rain gauge data and little or no suitable radar or satellite data are available. Two statistical methods—discriminant factorial analysis (DFA) and neural networks (NNs)—are used to determine 16 so-called elaborated nonlinear variables that best identify rainfall events related to banded orographic convection from atmospheric soundings. The approach takes rainfall information into account indirectly because it “learns” from the results of a previous study that explored meteorological and available rainfall databases, even if incomplete. The new variables include wind shear, low-level moisture fluxes, and gradients of the potential temperature in the lower layers of the atmosphere, and they were used to create an extensive database of banded orographic convection events from the archive of atmospheric soundings. Results of numerical simulations using the nonhydrostatic mesoscale (Méso-NH) meteorological model validate this approach and offer interesting perspectives for the understanding of the physical processes associated with banded orographic convection. DFA proves to be useful to determine the most discriminant factors with a physical meaning. Neural networks provide better results, but they do not allow for physical interpretation. The best solution is therefore to use the two methods together.
Multiple Equilibria of the Hadley Circulation in an Intermediate-Complexity Axisymmetric Model
Authors: Bellon Gilles , Sobel Adam H. 
Published: November 2009

Abstract
A model of intermediate complexity based on quasi-equilibrium theory—a version of the Quasi-Equilibrium Tropical Circulation Model with a prognostic atmospheric boundary layer, as well as two free-tropospheric modes in momentum, and one each in moisture and temperature—is used in a zonally symmetric aquaplanet configuration to study the sensitivity of the Hadley circulation to the sea surface temperature (SST) latitudinal distribution. For equatorially symmetric SST forcing with large SST gradients in the tropics, the model simulates the classical double Hadley cell with one equatorial intertropical convergence zone (ITCZ). For small SST gradients in the tropics, the model exhibits multiple equilibria, with one equatorially symmetric equilibrium and two asymmetric equilibria (mirror images of each other) with an off-equatorial ITCZ.
Further investigation of the feedbacks at play in the model shows that the assumed vertical structure of temperature variations is crucial to the existence and stability of the asymmetric equilibria. The free-tropospheric moisture–convection feedback must also be sufficiently strong to sustain asymmetric equilibria. Both results suggest that the specific physics of a given climate model condition determine the existence of multiple equilibria via the resulting sensitivity of the convection to free-tropospheric humidity and the vertical structure of adiabatic heating. The symmetry-breaking mechanism and resulting multiple equilibria have their origin in the local multiple equilibria that can be described by a single-column model using the weak temperature gradient approximation.
An additional experiment using an SST latitudinal distribution with a relative minimum at the equator shows that the feedbacks controlling these multiple equilibria might be relevant to the double-ITCZ problem.
Long-Range Propagation of the Semidiurnal Internal Tide from the Hawaiian Ridge
Authors: Zhao Zhongxiang , Alford Matthew H. , MacKinnon Jennifer A. , Pinkel Rob 
Published: November 2009

Abstract
The northeastward progression of the semidiurnal internal tide from French Frigate Shoals (FFS), Hawaii, is studied with an array of six simultaneous profiling moorings spanning 25.5°–37.1°N (≈1400 km) and 13-yr-long Ocean Topography Experiment (TOPEX)/Poseidon (T/P) altimeter data processed by a new technique. The moorings have excellent temporal and vertical resolutions, while the altimeter offers broad spatial coverage of the surface manifestation of the internal tide’s coherent portion. Together these two approaches provide a unique view of the internal tide’s long-range propagation in a complex ocean environment. The moored observations reveal a rich, time-variable, and multimodal internal tide field, with higher-mode motions contributing significantly to velocity, displacement, and energy. In spite of these contributions, the coherent mode-1 internal tide dominates the northeastward energy flux, and is detectable in both moored and altimetric data over the entire array. Phase and group propagation measured independently from moorings and altimetry agree well with theoretical values. Sea surface height anomalies (SSHAs) measured from moorings and altimetry agree well in amplitude and phase until the northern end of the array, where phase differences arise presumably from refraction by mesoscale flows. Observed variations in SSHA, energy flux, and kinetic-to-potential energy ratio indicate an interference pattern resulting from superposed northeastward radiation from Hawaii and southeastward from the Aleutian Ridge. A simple model of two plane waves explains most of these features.
The Effects of Tides and Oscillatory Winds on the Subtidal Inner-Shelf Cross-Shelf Circulation
Authors: Castelao Renato , Chant Robert , Glenn Scott , Schofield Oscar 
Published: November 2009

Abstract
A two-dimensional numerical model is used to investigate the effects of tidal forcing and oscillatory winds on the subtidal cross-shelf circulation on the inner shelf. Bottom topography and initial stratification are representative of the South and Middle Atlantic Bights along the U.S. east coast. Results from simulations forced by upwelling winds and no tides are consistent with previous studies of inner-shelf circulation. The inclusion of tidal forcing leads to increased mixing, larger eddy viscosity coefficients, and reduced stratification over the shallow regions, effectively reducing the wind efficiency to drive cross-shelf currents on the inner shelf. Tidally averaged cross-shelf currents are weaker compared to when no tides are considered. There is an increase in the width of the region of surface wind-driven transport divergence, which changes the cross-shelf location where upwelling occurs. Lagrangian analyses indicate that tidal forcing substantially reduces the transport of offshore waters toward the coast and increases the residence time over the inner shelf by up to 70%. Fluctuating winds with zero mean lead to a rectification of the cross-shelf flow on the inner shelf, resulting in net upwelling. The rectification occurs because the cross-shelf transport is nonzero during upwelling wind forcing (since dense water is brought to the inner shelf maintaining the stratification), but is approximately zero during downwelling winds (since surface water is forced under near-bottom water, destroying the stratification). The rectification is more clearly observed when stratification is strong, when tidal forcing is weak or absent, and when the wind fluctuates at low frequency.
Annual Reversal of the Equatorial Intermediate Current in the Pacific: Observations and Model Diagnostics
Authors: Marin Frédéric , Kestenare Elodie , Delcroix Thierry , Durand Fabien , Cravatte Sophie , Eldin Gérard , Bourdallé-Badie Romain 
Published: November 2009

Abstract
A large reversal of zonal transport below the thermocline was observed over a period of 6 months in the western Pacific Ocean between 2°S and the equator [from 26.2 Sv (1 Sv ≡ 106 m3 s−1) eastward in October 1999 to 28.6 Sv westward in April 2000]. To document this reversal and assess its origin, an unprecedented collection of ADCP observations of zonal currents (2004–06), together with a realistic OGCM simulation of the tropical Pacific, was analyzed. The results of this study indicate that this reversal is the signature of intense annual variability in the subsurface zonal circulation at the equator, at the level of the Equatorial Intermediate Current (EIC) and the Lower Equatorial Intermediate Current (L-EIC). In this study, the EIC and the L-EIC are both shown to reverse seasonally to eastward currents in boreal spring (and winter for the L-EIC) over a large depth range extending from 300 m to at least 1200 m. The peak-to-peak amplitude of the annual cycle of subthermocline zonal currents at 165°E in the model is ∼30 cm s−1 at the depth of the EIC, and ∼20 cm s−1 at the depth of the L-EIC, corresponding to a mass transport change as large as ∼100 Sv for the annual cycle of near-equatorial zonal transport integrated between 2°S and 2°N and between 410- and 1340-m depths. Zonal circulations on both sides of the equator (roughly within 2° and 5.5° in latitude) partially compensate for the large transport variability. The main characteristics of the annual variability of middepth modeled currents and subsurface temperature (e.g., zonal and vertical phase velocities, meridional structure) are consistent, in the OGCM simulation, with the presence, beneath the thermocline, of a vertically propagating equatorial Rossby wave forced by the westward-propagating component of the annual equatorial zonal wind stress. Interannual modulation of the annual variability in subthermocline equatorial transport is discussed.
Bias Correction and Bayesian Model Averaging for Ensemble Forecasts of Surface Wind Direction
Authors: Bao Le , Gneiting Tilmann , Grimit Eric P. , Guttorp Peter , Raftery Adrian E. 
Published: November 2009

AbstractWind direction is an angular variable, as opposed to weather quantities such as temperature, quantitative precipitation, or wind speed, which are linear variables. Consequently, traditional model output statistics and ensemble postprocessing methods become ineffective, or do not apply at all. This paper proposes an effective bias correction technique for wind direction forecasts from numerical weather prediction models, which is based on a state-of-the-art circular–circular regression approach. To calibrate forecast ensembles, a Bayesian model averaging scheme for directional variables is introduced, where the component distributions are von Mises densities centered at the individually bias-corrected ensemble member forecasts. These techniques are applied to 48-h forecasts of surface wind direction over the Pacific Northwest, using the University of Washington mesoscale ensemble, where they yield consistent improvements in forecast performance.
Negotiating the Pathways Into Care in a Globalizing World: Help-Seeking Behaviour of Ultra-Orthodox Jewish Parents
Authors: Schnitzer Gila , Loots Gerrit , Escudero Valentin , Schechter Isaac 
Published: November 2009

Background: The study of the pathways into care as a social process subject to a wide range of influences is needed to build appropriate and effective mental health services for culturally diverse societies. Material: Grounded theory and situational analysis of 21 in-depth interviews explores the help-seeking behaviour of ultra-Orthodox Jewish parents: which help-seeking pathways parents follow and how they make the decision to consult regular services for their child. Discussion: Three help-seeking pathways are influenced by glob/calization dynamics and gender: parents draw on diverse parenting discourses and strategies, socio-religious frameworks and cultural realities. Conclusion: Strategies are suggested to enhance the accessibility of services.
Outcomes comparison of 5 coated cardiopulmonary bypass circuits versus an uncoated control group of patients undergoing cardiac surgery
Authors: Marcoux J. , Sohn N. , McNair E. , Rosin M. , Smith G. , Lim H. , Mycyk T. , Meng Q. 
Published: November 2009

Attenuated inflammatory response and decreased platelet activation have been claimed repeatedly when biocompatible circuits are used for cardiopulmonary bypass. We evaluated five Health Canada approved biocompatible circuit coatings (BCC) against an un-coated control group to determine their effectiveness in improving post-operative outcomes. Patients were assigned to the Control group or one of the 5 coated circuit groups: 40 Control; 33 Trillium; 32 Phisio; 34 Bioline; 33 X; and 11 GBS. Measured outcomes included: ventilator time; ICU time; post-operative chest tube drainage and transfusion volume; high sensitivity C-reactive protein (hsCRP); tau protein; and pre- and 72-hour post-operative anti-saccadic eye movement test comparisons. Results: 183 patients were enlisted into the study. One arm of the study (GBS) was abandoned after 11 patients on account of inconsistent pressure excursions within the oxygenator and the excessive consumption of platelets necessitating transfusion. Patients in the X-coated group had significantly longer ventilator and intensive care unit (ICU) time compared to the three remaining coated circuit study groups. Though not significant, patients in the X group also demonstrated the highest post-operative chest tube losses, the most platelet transfusions, the highest tau protein levels and the lowest post-operative anti-saccadic eye movement test (ASEMT) results compared to the three remaining coated groups. The patients in the Trillium, Bioline and Phisio groups showed an improvement in ventilator and ICU time relative to the Control group. The diabetic patients in the Trillium, Bioline and Phisio groups showed an improvement in bleeding relative to the diabetic patients in the Control group. Conclusion: We compared all 5 coated circuits approved for clinical use in Canada against an uncoated control circuit. Three of the 5 coated circuits (Trillium, Phisio and Bioline BCC) were found to improve ventilator and ICU time compared to Control. Further studies are indicated to validate these results and their impact upon approval criteria, purchasing choices and safe clinical practice, especially as applied to higher risk diabetic patients.
Fear of falling, fall-related self-efficacy, anxiety and depression in individuals with chronic obstructive pulmonary disease
Authors: Hellström Karin , Vahlberg Birgit , Urell Charlotte , Emtner Margareta 
Published: November 2009

Objective: To examine the risk and frequency of falls, prevalence of fear of falling and activity avoidance, the magnitude of fall-related self-efficacy, and anxiety and depression in patients with chronic obstructive pulmonary disease. Design and settings: A cross-sectional study in patients with a diagnosis of chronic obstructive pulmonary disease who visited a lung clinic at a university hospital in Sweden. Subjects: Successive inclusion from autumn 2005 to spring 2006 of 80 patients with a mean age of 65 years. Intervention: Nil. Main measures: A questionnaire about fall history and consequences of fall, The Falls Efficacy Scale, Swedish version, and the Hospital Anxiety and Depression Scale were used. Results: Twenty patients (25%) reported at least one fall in the last year and 29% displayed fear of falling. Odds ratio for falling was 4—5 times higher in patients with severe chronic obstructive pulmonary disease. Older patients, women and patients with previous falls had a higher rate of fear of falling, and those with fear of falling had lower fall-related self-efficacy, increased level of anxiety and depression, more activity avoidance and use of assistive device. Conclusion: The increased risk of falls in patients with chronic obstructive pulmonary disease warrants attention in order to reduce serious and adverse health consequences of falls.
The effect of eating utensil weight on functional arm movement in people with Parkinson’s disease: a controlled clinical trial
Authors: Ma Hui-Ing , Hwang Wen-Juh , Tsai Pei-Luen , Hsu Yung-Wen 
Published: November 2009

Objective: To investigate the effect of eating utensil weight on kinematic performance in people with Parkinson’s disease. Design: A counterbalanced repeated-measures design. Setting: A motor control laboratory in a university setting. Subjects: Eighteen adults with Parkinson’s disease and 18 age-matched controls. Experimental conditions: Each participant performed a food transfer task using spoons of three different weights: lightweight (35 g), control (85 g) and weighted (135 g). Kinematic variables of arm movement were derived and compared between conditions. Main measures: Kinematic variables of arm movement, including movement time, peak velocity and number of movement units. Results: Utensil weights significantly affected the movement kinematics of all participants. Both groups had fewer movement units in the lightweight condition (Parkinson’s disease group: 22.18, controls: 19.89) than in the weighted condition (Parkinson’s disease group: 22.68, controls: 21.36), suggesting smoother movement in the former condition. In addition, both groups had higher peak velocity in the lightweight than in the weighted condition. Conclusions: Our findings suggest that a lightweight utensil may facilitate smoother and higher-velocity arm movement than a weighted one in people with Parkinson’s disease.
Differential Effects of Oral β Blockade on Cardiovascular and Sympathetic Regulation
Authors: Beloka S.P. , Gouveia S. , Gujic M. , Naeije R. , Rocha A.P. , van de Borne P. 
Published: November 2009

In patients with hypertension, β blockade decreases muscle sympathetic nerve activity (MSNA; micrographic technique) expressed in burst frequency (burst/min) but does not affect MSNA expressed in burst incidence (burst/100 heart beats), because reductions in blood pressure (BP) upon each diastole continue to deactivate the arterial baroreceptors, but at a slower heart rate (HR). We studied the effects of oral β blockade on MSNA and baroreflex sensitivity (BRS) in normal participants. Bisoprolol (5 mg, 1 week) was administered in 10 healthy young adults, using a double-blind, placebo-controlled, randomized cross-over study design. The beat-to-beat mean RR interval (RR) and systolic blood pressure (SBP) series were analyzed by power spectral analysis and power computation over the very low frequency (VLF), low frequency, and high frequency (HF) bands. Baroreflex sensitivity was computed from SBP and RR cross-analysis, using time and frequency domain methods. Bisoprolol increased RR (P < .0005), decreased mean SBP and diastolic blood pressure values (P < .01), did not change the SBP and RR powers, except for RR power in VLF (P < .02) and SBP power in HF (P < .03). The MSNA variability (P > .13) and respiratory pattern (P = .84) did not change from placebo to bisoprolol condition. The bisoprolol-induced bradycardia was associated with higher burst/100 heart beats (P < .05) and bisoprolol did not affect burst/min (P = .80). Time domain BRS estimates were increased after bisoprolol (P < .05), while frequency domain ones did not change (P > .1). Oral bisoprolol induces differential effects on sympathetic burst frequency and incidence in normal participants. Peripheral sympathetic outflow over time is preserved as a result of an increased burst incidence, in the presence of a slower HR. Unchanged BP and HR and MSNA variability suggests that the larger burst incidence is not due to sympathetic activation.