bluuebunny/crossref_metadata_2025_split huggingface dataset:
Direct Electroanalysis of p‐Nitrophenol (PNP) in Estuarine and Surface Waters by a High Sensitive Type C/p‐NiTSPc Coating Carbon Fiber Microelectrode (CFME)
Authors: Pontié M. , Sikpo L. , Thouand G. , Lahan R. , Tapsoba I. , Mallet R. , Feng T. 
Published: November 2010

AbstractWe report for the first time on the elaboration of a remarkable sensitivity type C/p‐NiTSPc carbon fiber microelectrode (CFME) for p‐nitrophenol (PNP) pollutant. We observed the best performances using square wave voltammetry (SWV) technique in the place of differential pulse voltammetry (DPV). Also the combination of p‐NiTSPc optimized cycles of electrodeposition and accumulation time, helped us to increase remarkably the sensor slope (320 times) and the limit of detection (LOD) (1000 times), with a final LOD of 0.1 µg/L, the current regulatory limit for drinkable waters. Furthermore the recoveries of the standards added are close to 100 % indicating that the method has some suitability. The proposed voltammetric procedure might be applied for organophosphorus pesticides and their by‐products with a remarkable sensing in natural polluted waters.
A shift approach for the dynamic response of rigid‐plastic systems
Authors: Voyagaki Elia , Mylonakis George , Psycharis Ioannis N. 
Published: November 2010

AbstractA shift approach is presented for evaluating and interpreting the response of rigid‐perfectly plastic single‐degree‐of‐freedom systems to dynamic loading. Scaling laws for such systems are, as the term suggests, multiplicative in nature, relating peak dynamic response to products of key problem parameters such as linear spectral coordinates, force reduction coefficient and peak values of the excitation and its time derivatives. Contrary to classical laws, the proposed approach is additive, imposing a shift in the ordinates and the abscissa of the excitation function by means of a set of parameters uniquely related to the yielding resistance of the system. The dynamic response is then obtained by integrating the modified excitation function in a linear‐like manner within a particular yielding branch, for the nonlinearity is incorporated into the forcing term. The mathematical validity of the approach is demonstrated analytically and its importance is highlighted for systems with symmetric yielding resistance subjected to near‐fault earthquake motions. The modified excitation function may be discontinuous between different yielding branches and relates uniquely to the development of plastic deformation. It is thereby referred to as Plastic Input Motion (PIM). It is shown that the ordinates and the duration of this function may be significantly (yet not necessarily) smaller than those of the original ground motion depending on yield strength. The relationship of the proposed approach to the existing methods and parameters of earthquake engineering such as Newmark's sliding block and relative ground acceleration, is discussed. Copyright © 2010 John Wiley & Sons, Ltd.
Increased adult hippocampal neurogenesis and abnormal migration of adult‐born granule neurons is associated with hippocampal‐specific cognitive deficits in phospholipase C‐β1 knockout mice
Authors: Manning Elizabeth E. , Ransome Mark I. , Burrows Emma L. , Hannan Anthony J. 
Published: November 2010

AbstractSchizophrenia is a devastating psychiatric illness with a complex pathophysiology. We have recently documented schizophrenia‐like endophenotypes in phospholipase C‐β1 knockout (PLC‐β1−/−) mice, including deficits in prepulse inhibition, hyperlocomotion, and cognitive impairments. PLC‐β1 signals via multiple G‐protein coupled receptor pathways implicated in neural cellular plasticity; however, adult neurogenesis has yet to be explored in this knockout model. In this study, weemployed PLC‐β1−/−mice to elucidate possible correlates between aberrant adult hippocampal neurogenesis (AHN) and schizophrenia‐like behaviors. Using stereology and bromodeoxyuridine (BrdU) immunohistochemistry we demonstrated a significant increase in the density of adult‐generated cells in the granule cell layer (GCL) of adult PLC‐β1−/−mice compared with wild‐type littermates. Cellular phenotype analysis using confocal microscopy revealed these cells to be mature granule neurons expressing NeuN and calbindin. Increased neuronal survival occurred concomitant with reduced caspase‐3(+) cells in the GCL of PLC‐β1−/−mice. Stereological analysis of Ki67(+) cells in the subgranular zone suggested that neural precursor proliferation is unchanged in PLC‐β1−/−mice. We further showed aberrant migration of mature granule neurons within the GCL of adult PLC‐β1−/−mice with excessive adult‐generated mature neurons residing in the middle and outer GCL. PLC‐β1−/−mice exhibited specific behavioral deficits in location recognition, a measure of hippocampal‐dependent memory, but not novel object recognition. Overall, we have shown that PLC‐β1−/−mice have a threefold increase in net AHN, and have provided further evidence to suggest a specific deficit in hippocampal‐dependent cognition. We propose that abnormal cellular plasticity in these mice may contribute to their schizophrenia‐like behavioral endophenotypes. © 2010 Wiley Periodicals, Inc.
Multiple uses of basement membrane‐like matrix (BME/Matrigel) in vitro and in vivo with cancer cells
Authors: Benton Gabriel , Kleinman Hynda K. , George Jay , Arnaoutova Irina 
Published: November 2010

AbstractSignificant advances in our understanding of cancer cell behavior, growth, and metastasis have been facilitated by studies using a basement membrane‐like extracellular matrix extract, also known as Matrigel. The basement membrane is a thin extracellular matrix that is found in normal tissues and contacts epithelial and endothelial cells, smooth muscle, fat, Schwann cells, etc. It is composed of mainly laminin‐111, collagen IV, heparan sulfate proteoglycan, entactin/nidogen, and various growth factors (fibroblast growth factor, transforming growth factor beta, epidermal growth factor, etc.). Most tumors of epithelial origin produce significant amounts of basement membrane matrix and interact with it particularly during metastasis. Cancer cells metastasize via degradation of the vessel basement membrane matrix to extravasate into the blood stream and colonize distant sites. This review will focus on the interaction of cancer cells and cancer stem cells with the basement membrane‐like matrix and the various uses of this interaction to accelerate tumor growth in vivo and to develop in vitro assays for invasion, morphology, and dormancy. Such assays and methods have advanced our understanding of the process of cancer progression, the genes and pathways that are involved, the potential of various therapeutic agents, the effects of neighboring cells, and the role of stem cells.
Functional role of inositol‐1,4,5‐trisphosphate‐3‐kinase‐A for motility of malignant transformed cells
Authors: Windhorst Sabine , Kalinina Tatyana , Schmid Katharina , Blechner Christine , Kriebitzsch Neele , Hinsch Robin , Chang Lydia , Herich Lena , Schumacher Udo , Mayr Georg W. 
Published: November 2010

AbstractCell migration is one of the hallmarks of metastatic disease and thus identification of migration promoting proteins is crucial for the understanding of metastasis formation. Here we show that the neuron‐specific, F‐actin bundling inositol‐1,4,5‐trisphosphate‐3‐kinase‐A (ITPKA) is ectopically expressed in tumor cells and critically involved in migration. Down‐regulation of ITPKA expression in transformed cell‐lines with ectopic expression of ITPKA significantly decreased migration and the number of linear and branched cell protrusion. Conversely, up‐regulation of ITPKA in tumor cell lines with low endogenous ITPKA expression increased migration and formation of cell processes. In vitro, ITPKA alone induced the formation of linear actin filaments, whereas ITPKA mediated formation of branched protrusions seems to result from interaction between ITPKA and the F‐actin cross‐linking protein filamin C. Based on these actin‐modulating and migration‐promoting effects of ITPKA we examined its expression in clinical samples of different tumor entities, starting with the analysis of multiple tumor tissue arrays. As in lung adenocarcinoma specimens, the highest ITPKA expression rate was found, this tumor entity was examined in more detail. ITPKA was expressed early in adenocarcinoma progression (pN0) and was largely maintained in invasive and metastatic tumor cell populations (pN1/2, lymph node metastases). Together with our result that high expression of ITPKA increases motility of tumor cells we conclude that the observed expression of ITPKA early in tumor development increases the metastatic potential of lung adenocarcinoma cells. Therefore, we suggest that ITPKA may be a promising therapeutic molecular target for anti metastatic therapy of lung cancer.
YB‐1 dependent virotherapy in combination with temozolomide as a multimodal therapy approach to eradicate malignant glioma
Authors: Holzmüller Regina , Mantwill Klaus , Haczek Cornelia , Rognoni Emanuel , Anton Martina , Kasajima Atsuko , Weichert Wilko , Treue Denise , Lage Hermann , Schuster Tibor , Schlegel Jürgen , Gänsbacher Bernd , Holm Per S. 
Published: November 2010

AbstractThe human Y‐box binding protein 1 (YB‐1) is known to be a promising target for cancer therapy. We have demonstrated that YB‐1 plays an important role in the adenoviral life cycle by regulating the adenoviral E2‐gene expression. Thus, we studied the oncolytic effect of the recombinant adenovirus Ad‐Delo3‐RGD, in which the transactivation domain CR3 of the E1A protein is ablated to enable viral replication only in YB‐1 positive cancer cells. In vitro Southern Blot analysis and cytopathic effect assays demonstrate high anti‐glioma potency, which was significantly increased in combination with temozolomide (TMZ), daunorubicin and cisplatin. Since vascular endothelial growth factor (VEGF) is thought to promote the hypervascular phenotype of primary, malignant brain tumors, we also tested Ad‐Delo3‐RGD in regard to the inhibition of VEGF expression. Indeed, we found that Ad‐Delo3‐RGD induced VEGF down regulation, which was even amplified under hypoxic conditions. Tumor‐bearing nudemice treated with the YB‐1 dependent oncolytic adenovirus showed significantly smaller tumors than untreated controls. Furthermore, combination therapy with TMZ led to a regression in all treated animals with complete tumor regression in 33 % of analyzed mice, which was verified by bioluminescence imaging and histological studies. In addition, histopathological evaluation revealed enhanced apoptosis and a reduction in tumor vessel formation, indicating that Ad‐Delo3‐RGD has an anti‐angiogenic effect in addition to its oncolytic capacity in vivo. Hence, our results demonstrate that the combination therapy of YB‐1 dependent virotherapy and TMZ is effective in a xenograft glioma mouse model and might be useful in a YB‐1 based clinical setting.
Effect of trisodium citrate on electrolytic deposition of hydroxyapatite coatings
Authors: Lin Dong‐Yang , Wang Xiao‐Xiang , Jiang Yong 
Published: November 2010

AbstractHydroxyapatite coatings were deposited on the titanium substrate by using various content Na3Cit as an organic modifier in electrolytes. The influence of the Na3Cit on the sizes of the HA crystals during electrolytic deposition was investigated under different molar ratio of Na3Cit to calcium ions. The experimental results showed that the size of HA crystal was well controlled by the addition of Na3Cit. When the molar ratio of Na3Cit to calcium ions was equal or greater than five, the obtained HA coating consisted of nanosized crystals. The HA nanoparticles were precipitated without a precursor phase when the molar ratio of Na3Cit to calcium ions was five; otherwise, the CaP coating experienced a phase conversion from octacalcium phosphate (OCP) to HA during the ELD. The effect of pH on saturation indexes with respect to hydroxyapatite, OCP, and dicalcium dihydrogen phosphate of these electrolytes was calculated by a computer program PHREEQC. The calculation showed the nucleation of OCP as the precursor during the deposition could be inhibited with increasing Na3Cit content in the electrolytes. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.
Biocompatibility and bioactivity enhancement of Ce stabilized ZrO2 doped HA coatings by controlled porosity change of Al2O3 substrates
Authors: Sima Felix , Ristoscu Carmen , Caiteanu Diana , Mihailescu Cristian N. , Stefan Nicolaie , Mihailescu Ion N. , Prodan Gabriel , Ciupina Victor , Palcevskis Eriks , Krastins Janis , Sima Livia E. , Petrescu Stefana M. 
Published: November 2010

AbstractAl2O3 substrates with controlled porosity were manufactured from nanosized powders obtained by plasma processing. It was observed that when increasing the sintering temperature the overall porosity was decreasing, but the pores got larger. In a second step, Ce stabilized ZrO2 doped hydroxyapatite coatings were pulsed laser deposited onto the Al2O3 substrates. It was shown that the surface morphology, consisting of aggregates and particulates in micrometric range, was altered by the substrate porosity and interface properties, respectively. TEM studies evidenced that Ce stabilized ZrO2 doped HA particulates ranged from 10 to 50 nm, strongly depending on the Al2O3 porosity. The coatings consisted of HA nanocrystals embedded in an amorphous matrix quite similar to the bone structure. These findings were congruent with the increased biocompatibility and bioactivity of these layers confirmed by enhanced growing and proliferation of human mesenchymal stem cells. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.
Reconstitution of human MCF‐7 breast cancer cells with caspase‐3 does not sensitize them to action of CDK inhibitors
Authors: Węsierska‐Gądek Józefa , Hackl Stefan , Zulehner Nora , Maurer Margarita , Komina Oxana 
Published: November 2010

AbstractHuman MCF‐7 breast cancer cells are resistant to pro‐apoptotic stimuli due to caspase‐3 inactivation. On the other hand, they should be sensitive to agents like selective pharmacological inhibitors of cyclin‐dependent kinases (CDKs) that (re)activate p53 tumor suppressor protein because they harbor intact p53 pathways. In this study we examined whether reconstitution of caspase‐3 in MCF‐7 cells sensitizes them to inhibitors of CDKs, by analyzing the effects of roscovitine (ROSC) and olomoucine (OLO), two closely related selective pharmacological CDK inhibitors, on both mother MCF‐7 cells and a secondary mutant line, MCF‐7.3.28 that stably expresses human caspase‐3. The results show that ROSC is, as expected, much more potent than OLO. Surprisingly; however, ROSC and OLO reduced proliferation of parental MCF‐7 cells more strongly than caspase‐3‐proficient counterparts. Both inhibitors arrest human breast cancer cells at the G2‐phase of the cell cycle. Analysis of cell‐cycle regulators by immunoblotting revealed that ROSC strongly induces p53 protein activity by inducing its phosphorylation at Ser46 in the MCF‐7 cells lacking caspase‐3, but not in caspase‐3‐proficient cells. Furthermore, reconstitution of caspase‐3 in MCF‐7 cells neither elevates the mitochondrial apoptosis rate nor significantly increases caspases activity upon ROSC treatment. However, the stabilization of p53 in response to DNA damaging agents is the same in both caspase negative and positive MCF‐7 cells. Cytotoxic agents induce caspase‐3‐dependent apoptosis in caspase‐3‐proficient cells. These results indicate that reconstitution of MCF‐7 cancer cells with caspase‐3 sensitize them to the action of DNA damaging agents but not to ATP‐like pharmacological inhibitors of CDKs. J. Cell. Biochem. 112: 277–288, 2011. © 2010 Wiley‐Liss, Inc.
Cognitive triad as mediator in the hopelessness model? a three‐wave longitudinal study
Authors: Pössel Patrick , Thomas S. Denise 
Published: November 2010

AbstractSeveral authors proposed that all elements of Beck's cognitive triad (1976) mediate the associations between inference style as described in the hopelessness model (Abramson, Alloy, & Metalsky, 1989) and depressive symptoms. Results of a 3‐wave longitudinal study indicate only a partial mediation model with all elements of the cognitive triad being associated with all inference styles, with depressive symptoms fitting the data best. Controlling for direct and indirect effects, no individual element of the cognitive triad mediates the association between inference styles and depressive symptoms. The partial mediation model is not stable across sex or clinical vs subclinical samples. In general, the data supports the integration of all three elements of the cognitive triad into the hopelessness model. © 2010 Wiley Periodicals, Inc. J Clin Psychol 00:1–7, 2010.
‘Youthscapes’ and escapes in rural Africa: Education, mobility and livelihood trajectories for young people in Eastern Cape, South Africa
Authors: Porter Gina , Hampshire Kate , Mashiri Mac , Dube Sipho , Maponya Goodhope 
Published: November 2010

AbstractThis paper draws attention to the significance of mobility in shaping the educational and livelihood trajectories of rural young people in sub‐Saharan Africa, with particular reference to a case study in Eastern Cape, South Africa. Young rural people commonly face both economic and political exclusions. As our case study illustrates, in the context of work demands, restricted basic education and poverty, the potential for escape, whether to secondary education or city jobs, is limited and in some respects highly gendered. We draw principally on ethnographic material from interviews with people aged 9–18 years, their parents, teachers and other key informants. Copyright © 2010 John Wiley & Sons, Ltd.
Phenolic compounds in seed oils
Authors: Van Hoed Vera 
Published: November 2010

AbstractTraditionally, phenolic compounds are known to be abundant in fruits, vegetables and cereals. Recently, however, their presence in seed oils has been discovered and this offers interesting nutritional and economical possibilities. The nutritional benefit arises from the high levels of polar antioxidants in crude seed oils, as it is known that these are antioxidants offering health benefits. Economical benefit results when seeds traditionally considered as a waste stream become the source of high value vegetable oils. The main identified phenolic compounds in those oils are discussed, as well as the varying levels of total phenolic compounds as determined by the Total Phenol Content (TPC) assay and by HPLC methods. While not all compounds are yet identified, and further study is needed on this subject, it is clear that the phenolic compounds in seed oils contribute to their oxidative stability and to their nutritional importance. The available data show that phenolic compounds are not only abundant in fruits, cereals and vegetables, but seed oils are also good sources of a variety of these antioxidants, in particular phenolic acids. This gives possibilities for the use of otherwise waste streams, such as fruit seeds, as sources of high value oils, with interesting nutritional properties, including essential fatty acids and antioxidants.
Microbial production of rare unsaturated fatty acids and alcohols
Authors: Nagao Toshihiro , Shimada Yuji 
Published: November 2010

AbstractAeromonas hydrophila N‐6, isolated from a soil sample, converted vegetable oils to several rare unsaturated fatty acids and alcohols accumulated inside the cells as a wax ester form. A. hydrophila N‐6 effectively decreased fatty acid chain lengths, and converted rapeseed, safflower and linseed oils into 7‐16:1 and 5‐14:1 fatty acids, 7,10‐16:2 and 5,8‐14:2 fatty acids, and 7,10,13‐16:3 fatty acids, respectively. Furthermore, A. hydrophila N‐6 reduced the resulting fatty acids to rare unsaturated fatty alcohols, such as 7‐16:1, 5‐14:1, 9,12‐18:2, 7,10‐16:2, 9,12,15‐18:3 and 7,10,13‐16:3. Such unsaturated fatty acids and alcohols are rarely found in natural oils. Because decreasing fatty acid carbon chain lengths from the carboxyl end and reducing unsaturated fatty acids to unsaturated fatty alcohols in industrially applicable scale are both difficult reactions to accomplish by chemical means, we suggest that A. hydrophila N‐6 may facilitate the introduction of new bioprocesses for producing rare unsaturated fatty acids and alcohols, especially fatty alcohols with more than two double bonds.
Can blood cholesterol levels be too low?
Authors: Stanley John 
Published: November 2010

AbstractLike glucose, cholesterol is an essential cellular nutrient and like glucose, blood cholesterol levels need to be kept within limits. The deleterious effects of hypercholesterolaemia on the risk of cardiovascular disease are well established and have resulted in the widespread prescription of statins to lower blood cholesterol levels and hence lower the risk of developing the disease. However, the possible deleterious effects of hypocholesterolaemia on mental health are less well studied and while there is enough evidence to cause concern, there is not enough evidence to be sure that these deleterious effects really exist. More research is urgently needed and in particular studies which investigate the possibility that LDL cholesterol and HDL cholesterol have different effects on mental health as they do on cardiovascular health.
Countably Categorical Structures with n‐Degenerate Algebraic Closure
Authors: Vassiliev Evgueni V. 
Published: November 2010

AbstractWe study the class of ω‐categorical structures with n‐degenerate algebraic closure for some n ε ω, which includes ω‐categorical structures with distributive lattice of algebraically closed subsets (see [4]), and in particular those with degenerate (trivial) algebraic closure. We focus on the models of ω‐categorical universal theories, absolutely ubiquitous structures, and ω‐categorical structures generated by an indiscernible set. The assumption of n‐degeneracy implies total categoricity for the first class, stability for the second, and ω‐stability for the third.
On the Consistency of a Positive Theory
Authors: Esser Olivier 
Published: November 2010

AbstractIn positive theories, we have an axiom scheme of comprehension for positive formulas. We study here the “generalized positive” theory GPK∞+. Natural models of this theory are hyperuniverses. The author has shown in [2] that GPK∞+ interprets the Kelley Morse class theory. Here we prove that GPK∞+ + ACWF (ACWF being a form of the axiom of choice allowing to choose elements in well‐founded sets) and the Kelley‐Morse class theory with the axiom of global choice and the axiom “On is ramifiable” are mutually interpretable. This shows that GPK∞+ + ACWF is a “strong” theory since “On is ramifiable” implies the existence of a proper class of inaccessible cardinals.