bluuebunny/crossref_metadata_2025_split huggingface dataset:
A multiplexed bead assay for profiling glycosylation patterns on serum protein biomarkers of pancreatic cancer
Authors: Li Chen , Zolotarevsky Eugene , Thompson Ian , Anderson Michelle A. , Simeone Diane M. , Casper John M. , Mullenix Michael C. , Lubman David M. 
Published: July 2011

AbstractA multiplexed bead‐based immunoassay was developed to simultaneously profile glycosylation patterns of serum proteins to investigate their usefulness as biomarkers for pancreatic cancer. The multiplex assay utilized protein‐specific capture antibodies chemically coupled individually to beads labeled with specific amounts of fluorescent dye. Captured proteins were detected based on the extent and specific type of glycosylation as determined by successive binding of fluorescent lectin probes. Advantages to this technique include the fact that antibodies coupled to the beads had minimal nonspecific binding to the lectins ConA/SNA, avoiding the step of chemically blocking the antibody glycans and the bead assays were performed in a 96‐well filter plate enabling high‐throughput screening applications with improved reproducibility. The assay was tested with ConA and SNA lectins to examine the glycosylation patterns of α‐1‐β glycoprotein (A1BG) and serum amyloid p (SAP) component for use as potential biomarkers for the detection of pancreatic cancer based on the results from prior biomarker studies. The results showed that the SNA response on the captured A1BG protein could distinguish chronic pancreatitis samples from pancreatic cancer with a p‐value of 0.035 and for the SAP protein with SNA, a p‐value of 0.026 was found between the signal of normal controls and the pancreatic cancer samples. For the ConA response, a decline in the signal for both proteins in the serum samples was found to distinguish pancreatic cancer from normal controls and renal cell carnoma samples (A1BG, p<0.05; and SAP, p<0.0001).
A Practical Comparison of Two Types of Family Intervention: An Exploratory RCT of Family Day Workshops and Individual Family Work as a Supplement to Inpatient Care for Adults with Anorexia Nervosa
Authors: Whitney Jenna , Murphy Tara , Landau Sabine , Gavan Kay , Todd Gill , Whitaker Wendy , Treasure Janet 
Published: July 2011

AbstractBackgroundLittle is known about the outcome of involving families in the treatment of adults with anorexia nervosa. Carers of people with anorexia nervosa experience significant levels of distress. This may contribute to unhelpful behaviours which maintain the illness.AimsTo evaluate the effectiveness and acceptability of family workshops with educational and skills‐based components as compared to individual family work.MethodAn exploratory randomised controlled trial of two forms of family intervention was conducted for inpatients with anorexia nervosa (n = 48) and their family members on a specialised unit for adults.ResultsIn both groups, there was an improvement in patients' BMI and a reduction in carers' distress. There were no differences between groups.ConclusionPreliminary findings suggest that workshop‐based intervention with two families might be as effective as an individually focused family intervention. This may offer a more cost‐effective intervention for this treatment‐resistant group. Copyright © 2011 John Wiley & Sons, Ltd and Eating Disorders Association.
Family Work in Anorexia Nervosa: A Qualitative Study of Carers' Experiences of Two Methods of Family Intervention
Authors: Whitney Jenna , Currin Laura , Murray Joanna , Treasure Janet 
Published: July 2011

AbstractBackgroundQualitative methods can aid in the development of complex interventions.AimsQualitative methodology was used to examine treatment efficacy, carer satisfaction and process of change in carers of people with anorexia nervosa who participated in a randomised controlled trial (RCT) comparing individual family work (IFW) and multi‐(dual) family workshops.MethodTwenty‐three carers were interviewed. Themes were developed using Interpretive Phenomenological Analysis (IPA).ResultsCarers who worked with another family described this as beneficial. There was dissatisfaction about a lack of structure perceived in the IFW. Most carers felt the interventions helped them communicate, understand the illness and gain personal and familial insight. Although there was lack of consensus on the most helpful techniques, carers felt sessions should focus on individual relationships and practical guidance for challenging behaviour.ConclusionsCarers valued structured, skills‐based interventions. The inclusion of multiple families may reduce carer blame and isolation and facilitate skills‐based learning. Copyright © 2011 John Wiley & Sons, Ltd and Eating Disorders Association.
Application of the variational iteration method to nonlinear non‐Fourier conduction heat transfer equation with variable coefficient
Authors: Saedodin Seyfolah , Yaghoobi Hessameddin , Torabi Mohsen 
Published: July 2011

AbstractIn this paper, a variational iteration method (VIM) has been applied to nonlinear non‐Fourier conduction heat transfer equation with variable specific heat coefficient. The concept of the variational iteration method is introduced briefly for applying this method for problem solving. The proposed iterative scheme finds the solution without any discretization, linearization, or restrictive assumptions. The results of VIM as an analytical solution are then compared with those derived from the established numerical solution obtained by the fourth order Runge–Kutta method in order to verify the accuracy of the proposed method. The results reveal that the VIM is very effective and convenient in predicting the solution of such problems, and it is predicted that VIM can find a wide application in new engineering problems. © 2011 Wiley Periodicals, Inc. Heat Trans Asian Res; Published online in Wiley Online Library (wileyonlinelibrary.com/journal/htj). DOI 10.1002/htj.20362
Behavioral study of alumina nanoparticles in pool boiling heat transfer on a vertical surface
Authors: Hegde Ramakrishna N. , Reddy R.P. , Rao Srikanth S. 
Published: July 2011

AbstractExperiments were carried out to investigate the pool boiling of alumina‐water nanofluid at 0.1 g/l to 0.5 g/l of distilled water, and the nucleate pool boiling heat transfer of pure water and nanofluid at different mass concentrations were compared at and above the atmospheric pressure. At atmospheric pressure, different concentrations of nanofluids display different degrees of deterioration in boiling heat transfer. The effect of pressure and concentration of nanoparticles revealed significant enhancement in heat flux and deterioration in pool boiling. The heat transfer coefficient of 0.5 g/l alumina‐water nanofluid was compared with pure water and clearly indicates deterioration. At all pressures the heat transfer coefficients of the nanofluid were lower than those of pure water. Experimental observation revealed particles coating over the heater surface and subsequent SEM inspection of the heater surface showed nanoparticles coating on the surface forming a porous layer. To substantiate the nanoparticle deposition and its effect on heat flux, investigation was done by measuring the surface roughness of the heater surface before and after the experiment. While SEM images of the heater surface revealed nanoparticle deposition, surface roughness of the heater surface confirmed it. Based on the experimental investigations it can be concluded that an optimum thickness of nanoparticles coating favors an increase in heat flux. Higher surface temperature due to the presence of nanoparticles coating results in the deterioration of boiling heat transfer. © 2011 Wiley Periodicals, Inc. Heat Trans Asian Res; Published online in Wiley Online Library (wileyonlinelibrary.com/journal/htj). DOI 10.1002/htj.20365
Delineation of reservoirs using remote sensing and their storage estimate: an example of the Yellow River basin, China
Authors: Ran Lishan , Lu X. X. 
Published: July 2011

AbstractReservoirs are an integral component of water resources planning and management. Periodic and accurate assessment of the water storage change in reservoirs is an extraordinarily important aspect for better watershed management and water resources development. In view of the shortcomings of conventional approaches in locating reservoirs' spatial location and quantifying their storage, the remote sensing technique has several advantages, either for a single reservoir or for a group of reservoirs. The satellite‐based remote sensing data, encompassing spatial, spectral and temporal attributes, can provide high‐resolution synoptic and repetitive information with short time intervals on a large scale. Using remote sensing images in conjunction with Google Earth and field check of representative reservoirs, the spatial distribution of constructed reservoirs in the Yellow River basin was delineated, and their storage volume and the residence time of the stored water were estimated. The results showed that 2816 reservoirs were extracted from the images, accounting for 89·5% of the registered total. All large‐ and medium‐sized reservoirs were extracted while small reservoirs may not be extracted due to coarse resolution and cloud‐cover shadows. An empirical relationship between the extracted water surface area and the compiled storage capacity of representative reservoirs was developed. The water storage capacity was estimated to be 66·71 km3, about 92·7% of the total storage capacity reported by the authority. Furthermore, the basin was divided into 10 sub‐basins upon which the water's residence time was analysed. The water discharge in the basin has been greatly regulated. The residence time has surged to 3·97 years in recent years, ranking the Yellow River in the top three of the list in terms of residence time and flow regulation among large river systems in the world. It is expected that it will be further extended in future owing to decreasing water discharge and increasing reservoir storage capacity. Copyright © 2011 John Wiley & Sons, Ltd.
Epigenetic regulation of osteoclast differentiation: Possible involvement of Jmjd3 in the histone demethylation of Nfatc1
Authors: Yasui Tetsuro , Hirose Jun , Tsutsumi Shuichi , Nakamura Kozo , Aburatani Hiroyuki , Tanaka Sakae 
Published: July 2011

Abstract
Gene expression is controlled by epigenetic mechanisms such as histone acetylation and methylation, and recent studies have revealed that key developmental steps are regulated by the trimethylation of histone H3 lysine 4 (H3K4me3) and lysine 27 (H3K27me3). Using ChIP sequencing technology combined with real-time PCR, we here demonstrate that the H3K27me3 observed in the Nfatc1 gene in bone marrow–derived macrophages (BMMs) was markedly reduced in mature osteoclasts. Jumonji domain-containing 3 (Jmjd3), a H3K27 demethylase, was induced in bone marrow–derived macrophages and in the vicinity of the transcription start site (TSS) of nuclear factor–activated T cells (NFAT) c1 in response to receptor activator of nuclear factor-κB ligand (RANKL) stimulation. Gene silencing of the Jmjd3 gene by short hairpin RNA reduced demethylation of H3K27me3 at the TSS of Nfatc1 and suppressed RANKL-induced osteoclastogenesis. These results suggest that the demethylation of H3K27me3 in the Nfatc1 gene locus by Jmjd3 plays a critical role in RANKL-induced osteoclast differentiation. © 2011 American Society for Bone and Mineral Research
Counterpoise correction is not useful for short and Van der Waals distances but may be useful at long range
Authors: Sheng Xiao Wei , Mentel Lukasz , Gritsenko Oleg V. , Baerends Evert Jan 
Published: July 2011

AbstractThis article investigates the errors in supermolecule calculations for the helium dimer. In a full CI calculation, there are two errors. One is the basis set superposition error (BSSE), the other is the basis set convergence error (BSCE). Both of the errors arise from the incompleteness of the basis set. These two errors make opposite contributions to the interaction energies. The BSCE is by far the largest error in the short range and larger than (but much closer to) BSSE around the Van der Waals minimum. Only at the long range, the BSSE becomes the larger error. The BSCE and BSSE largely cancel each other over the Van der Waals well. Accordingly, it may be recommended to not include the BSSE for the calculation of the potential energy curve from short distance till well beyond the Van der Waals minimum, but it may be recommended to include the BSSE correction if an accurate tail behavior is required. Only if the calculation has used a very large basis set, one can refrain from including the counterpoise correction in the full potential range. These results are based on full CI calculations with the aug‐cc‐pVXZ (X = D, T, Q, 5) basis sets. © 2011 Wiley Periodicals, Inc. J Comput Chem 32: 2896–2901, 2011
Bioproduction of cis‐(1S,2R)‐indandiol, a chiral pharmaceutical intermediate, using a solid–liquid two‐phase partitioning bioreactor for enhanced removal of inhibitors
Authors: Dafoe Julian T S , Daugulis Andrew J 
Published: July 2011

AbstractBACKGROUND: A solid‐liquid two‐phase partitioning bioreactor (TPPB) was used in the biotransformation of indene to cis‐(1S,2R)‐indandiol by Pseudomonas putida 421‐5 (ATCC 55687). Metered substrate feeding in single‐phase operation, or delivery from an immiscible liquid, have previously been employed to regulate the exposure of the biocatalyst to inhibitory concentrations of the substrate. In contrast, the solid‐liquid platform provided in situ substrate addition (ISSA) as well as simultaneous it in situ product removal (ISPR) as a means of overcoming substrate and product toxicity. Three different modes of operation were compared for their effects on the performance of this biotransformation: single‐phase, fed‐batch operation was carried out as a benchmark in 2.75 L aqueous medium, and subsequently with the inclusion of either 700 g liquid silicone oil or 700 g solid polymer beads.RESULTS: Biphasic modes achieved a 3‐fold productivity improvement with respect to single‐phase (30 to 90 mg L−1 h−1), and solid‐liquid productivity was similar to liquid‐liquid operation while achieving more extensive removal of inhibitory compounds resulting in a slightly higher product titer (1.29 vs 1.16 g L−1). The operability of the reactor was improved by the phase stability of the solid polymer beads relative to immiscible organic solvents, preventing emulsion formation and facilitating analytics.CONCLUSION: Solid polymer beads replaced the immiscible liquid auxiliary phase for substrate delivery while performing simultaneous inhibitory molecule sequestration. Copyright © 2011 Society of Chemical Industry
Citric acid, biomass and cellular lipid production by Yarrowia lipolytica strains cultivated on olive mill wastewater‐based media
Authors: Sarris Dimitris , Galiotou‐Panayotou Maria , Koutinas Apostolis A. , Komaitis Michael , Papanikolaou Seraphim 
Published: July 2011

AbstractBACKGROUND: Olive mill wastewaters (OMWs) are an important residue and several physico‐chemical and/or biotechnological methods have been proposed for their treatment.RESULTS: The ability of three Yarrowia lipolytica strains to grow on and convert glucose‐enriched OMWs into added‐value compounds in carbon‐ and nitrogen‐limited shake‐flask cultures was assessed. Remarkable decolorization (up to 63%) and non‐negligible removal of phenolic compounds (up to 34%, w/w) occurred. In nitrogen‐limited cultures, the accumulation of cellular lipids was favored by OMW addition into the medium. In contrast, although remarkable quantities of citric acid (Cit) were produced in control experiments (cultures without OMW addition), in which Cit up to 18.9 g L−1 was produced with yield of Cit synthesized per sugar consumed ∼0.73 g g−1), adaptation of cultures to media supplemented with OMWs reduced the final Cit quantity and conversion yield values achieved. In OMW‐based media, the highest concentration of citric acid produced was 18.1 g L−1, with conversion yield ∼0.51 g g−1. In carbon‐limited cultures, despite the presence of inhibitory compounds in the medium (e.g. phenols), biomass production was enhanced with the addition of OMWs. The highest biomass concentration achieved was 12.7 g L−1, with biomass conversion yield per sugar consumed ∼0.45 g g−1. Fatty acid analysis of cellular lipid produced demonstrated that adaptation of all strains in OMW‐based media favored the synthesis of cellular lipids that contained increased concentrations of cellular oleic acid.CONCLUSIONS: The Y. lipolytica strains tested can be regarded as possible candidates for simultaneous OMWs remediation and production of added‐value compounds. Copyright © 2011 Society of Chemical Industry
Molecular determinants of disease in coxsackievirus B1 murine infection
Authors: Cifuente Javier O. , Ferrer María F. , de Giusti Carolina Jaquenod , Song Wen‐Chao , Romanowski Víctor , Hafenstein Susan L. , Gómez Ricardo M. 
Published: July 2011

AbstractTo understand better how different genomic regions may confer pathogenicity for the coxsackievirus B (CVB), two intratypic CVB1 variants, and a number of recombinant viruses were studied. Sequencing analysis showed 23 nucleotide changes between the parental non‐pathogenic CVB1N and the pathogenic CVB1Nm. Mutations present in CVB1Nm were more conserved than those in CVB1N when compared to other CVB sequences. Inoculation in C3H/HeJ mice showed that the P1 region is critical for pathogenicity in murine pancreas and heart. The molecular determinants of disease for these organs partially overlap. Several P1 region amino acid differences appear to be located in the decay‐accelerating factor (DAF) footprint CVBs. CVB1N and CVB1Nm interacted with human CAR, but only CVB1N seemed to interact with human DAF, as determined using soluble receptors in a plaque‐reduction assay. However, the murine homolog Daf‐1 did not interact with any virus assessed by hemagglutination. The results of this study suggest that an unknown receptor interaction with the virus play an important role in the pathogenicity of CVB1Nm. Further in vivo studies may clarify this issue. J. Med. Virol. 83:1571–1581, 2011. © 2011 Wiley‐Liss, Inc.
Case report: Detection of rotavirus RNA in the cerebrospinal fluid of a child with rotavirus gastroenteritis and meningism
Authors: Medici Maria Cristina , Abelli Laura Anna , Guerra Paola , Dodi Icilio , Dettori Giuseppe , Chezzi Carlo 
Published: July 2011

AbstractAlthough case reports have described detection of rotavirus (RV) in extraintestinal sites such as the liver, kidney, and central nervous system (CNS) of children with RV gastroenteritis, CNS localization in RV infection seems to be rare. RT‐PCR and nucleotide sequencing detected a G1P[8] strain in the stool and cerebrospinal fluid (CSF) samples of a patient with concurrent RV‐associated enteritis and CNS signs. Upon sequence analysis, the viruses detected in the CSF was identical to the virus detected in the stools. In the VP7‐ and VP4‐based phylogenetic dendograms the strain clustered within the G1‐Ic sub‐lineage and the P[8]‐III lineage. This study supports the hypothesis that RV infection was able to spread from the intestinal tract to the CNS, and likely played a role in the onset of neurological disease. J. Med. Virol. 83:1637–1640, 2011. © 2011 Wiley‐Liss, Inc.
On the existence of positive periodic solutions of systems of second order differential equations
Authors: Li Xiong , Zhang Ziheng 
Published: July 2011

AbstractIn this paper, we establish several criteria for the existence, multiplicity, nonexistence of positive periodic solutions of the following system

by combining some new properties of Green's function together with Krasnosel'skĭ fixed point theorem on the compression and expression of cones. © 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim
Health‐related quality‐of‐life scales in Parkinson's disease: Critique and recommendations
Authors: Martinez‐Martin Pablo , Jeukens‐Visser Martine , Lyons Kelly E. , Rodriguez‐Blazquez C. , Selai Caroline , Siderowf Andrew , Welsh Mickie , Poewe Werner , Rascol Oliver , Sampaio Cristina , Stebbins Glenn T. , Goetz Christopher G. , Schrag Anette 
Published: July 2011

AbstractHealth‐related quality of life is an important patient‐reported outcome used in intervention trials and for monitoring the consequences of health status on physical, mental, and social domains. Parkinson's disease is a complex disorder that strongly affects patients' quality of life. Several health‐related quality of life tools have been used in Parkinson's disease. A Movement Disorder Society Task Force was commissioned to rate the psychometric quality of available health‐related quality of life scales as applied to Parkinson's disease. Following the methodology adopted by previous work of the Movement Disorder Society Task Force, a review of generic and specific health‐related quality of life scales applied in studies on Parkinson's disease was completed. Considering the scales from 3 perspectives—use in Parkinson's disease, use by multiple research groups, and clinimetric properties—a final classification as “recommended,” “suggested,” or “listed” was applied to each reviewed instrument. Four generic scales (EuroQoL, Nottingham Health Profile, 36‐Item Short‐Form Health Survey, and Sickness Impact Profile) and 5 specific scales (39‐Item Parkinson's Disease Questionnaire, Parkinson's Disease Questionnaire Short Form, Parkinson's Disease Quality of Life Questionnaire, Parkinson's Impact Scale, and Scales for Outcomes in Parkinson's Disease–Psychosocial) reached the level of “recommended.” The 39‐item Parkinson's Disease Questionnaire is the most thoroughly tested and applied questionnaire. Three other generic measures (Quality of Life Questionnaire 15D, Schedule for the Evaluation of Individual Quality of Life‐Direct Weighting, and World Health Organization Quality of Life Assessment Short Version) and the specific Parkinson's Disease Quality of Life Scale are “suggested.” With a little additional effort in completing the stipulated requirements, they could reach the “recommended” level. At present there is a wide variety of health‐related quality of life measures for application in the Parkinson's disease setting, and the task force does not recommend the development of a new scale. Selection of the most appropriate instrument for a particular objective requires consideration of the characteristics of each scale and the goals of the assessment. © 2011 Movement Disorder Society
Arm tremor in cervical dystonia—Is it a manifestation of dystonia or essential tremor?
Authors: Schiebler Sarah , Schmidt Alexander , Zittel Simone , Bäumer Tobias , Gerloff Christian , Klein Christine , Münchau Alexander 
Published: July 2011

AbstractThe classification of arm tremor in cervical dystonia is a controversial issue. There have been many, at times passionate disputes in the movement disorder community about whether it should be classified as a manifestation of dystonia or essential tremor associated with dystonia. There are arguments in favor of both views. Settling the issue might be relevant to the understanding of the etiological, presumably genetic, background because phenomenological grouping is the starting point for genetic analyses. From this point of view, we outline this tremor debate and add some new clinical data. © 2011 Movement Disorder Society