bluuebunny/crossref_metadata_2025_split huggingface dataset:
IFN-αβ and Self-MHC Divert CD8 T Cells into a Distinct Differentiation Pathway Characterized by Rapid Acquisition of Effector Functions
Authors: Marshall Heather D. , Prince Amanda L. , Berg Leslie J. , Welsh Raymond M. 
Published: July 2010

AbstractNonvirus-specific bystander CD8 T cells bathe in an inflammatory environment during viral infections. To determine whether bystander CD8 T cells are affected by these environments, we examined P14, HY, and OT-I TCR transgenic CD8 T cells sensitized in vivo by IFN-αβ–inducing viral infections or by polyinosinic:polycytidylic acid. These sensitized cells rapidly exerted effector functions, such as IFN-γ production and degranulation, on contact with their high-affinity cognate Ag. Sensitization required self-MHC I and indirect effects of IFN-αβ, which together upregulated the T-box transcription factor Eomesodermin, potentially enabling the T cells to rapidly transcribe CTL effector genes and behave like memory cells rather than naive T cells. IL-12, IL-15, IL-18, and IFN-γ were not individually required for sensitization to produce IFN-γ, but IL-15 was required for upregulation of granzyme B. These experiments indicate that naive CD8 T cells receive signals from self-MHC and IFN-αβ and that, by this process, CD8 T cell responses to viral infection can undergo distinct differentiation pathways, depending on the timing of Ag encounter during the virus-induced IFN response.
Synthesis and characterization of PNIPAM/PS core/shell particles
Authors: Zhang Li , Daniels Eric S. , Dimonie Victoria L. , Klein Andrew 
Published: June 2010

AbstractCrosslinked, monodisperse PNIPAM particles were synthesized by precipitation polymerization. The particle size was measured by dynamic light scattering (DLS), capillary hydrodynamic fractionation (CHDF), and transmission electron microscopy (TEM). Two different polymerization methods were used to prepare PNIPAM/PS core/shell particles, both above and below the volume phase transition temperature (VPPT) using either a semibatch or seeded semibatch polymerization process. In both processes, uniform “raspberry” structures were obtained in which polystyrene formed small domains on the surface of the PNIPAM particles. The resulting core and shell structure was confirmed by temperature‐dependent particle size and density gradient experiments. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010
Flocculation performance of modified chitosan in an aqueous suspension
Authors: Ali Sk Akbar , Pal Sagar , Singh Ram Prakash 
Published: June 2010

AbstractA cationic moiety, N‐(3‐chloro‐2‐hydroxypropyl)trimethyl ammonium chloride (CHPTAC), was incorporated onto chitosan in an aqueous alkaline solution. Thus, modified chitosan was prepared. A series of modified chitosans with different molecular weights and charge densities were synthesized through the alteration of the molar ratio of CHPTAC to chitosan in the reaction mixture. The synthesized modified chitosans were thereafter characterized by a variety of physicochemical characterization techniques to confirm that modification did take place. Furthermore, the feasibility of applying modified chitosans as flocculants was assessed, and they were compared with the native chitosan in model colloidal suspensions of kaolin and iron‐ore powder. The results indicated that the unmodified chitosan itself was a good flocculating agent. The flocculation performance of the chitosan could be altered by the incorporation of the CHPTAC moiety. Studies showed that not all the modified chitosans had superior flocculation performance versus the native chitosan. Among the different grades, the modified chitosan with a moderate molecular weight and a moderate charge density showed the best flocculation performance in both model suspensions. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010
Conservation genetics of the endangered depressed river mussel, Pseudanodonta complanata, using amplified fragment length polymorphism (AFLP) markers
Authors: Skidmore Richard , Leach Christopher , Hoffman Joe , Amos William , Aldridge David 
Published: June 2010

Abstract
Genetic analysis is increasingly recognized as a key tool for understanding demography, and is particularly useful for describing patterns of gene flow between putative populations. Most effort has been directed towards vertebrate systems, where any one study often benefits from marker development in related species. The greater diversity of invertebrate taxa presents a challenge, but amplified fragment length polymorphism (AFLP) markers offer a solution, yielding high levels of polymorphism and no prior knowledge of a species' genome.
AFLP markers have been used to analyse an unusual metapopulation of an invertebrate, the endangered freshwater mussel, Pseudanodonta complanata, sampled from river systems across the UK. This was done to assess the extent to which individual river systems were genetically isolated from one another.
The results show patterns of weak genetic differentiation across the UK, with one hydrologically isolated population in the south west showing clear genetic differentiation from the rest of the country. However, the UK population as a whole exhibits significant isolation by distance, particularly when one population subject to fish stocking is removed, this population probably being seeded with mussel glochidia larvae which use fish as vectors. Genetic estimates of inbreeding reveal a complicated pattern in which inbreeding peaks at intermediate densities. High‐density populations may be genetically diverse due to their size, while the lowest density populations may represent transient groups of emigrants from other, larger populations.
The findings show that limited gene flow does exist between some but not all river systems. The isolation of the south‐west population indicates that dispersal is variable and should not be assumed to be present. Waterways that remain hydrologically isolated may require special attention in conservation programmes as they can harbour genetically distinct populations. The balance between river management activities and conservation priorities therefore needs careful consideration. Copyright © 2010 John Wiley & Sons, Ltd.
Design and use of peptide‐based antibodies decreasing superoxide production by mitochondrial complex I and complex II
Authors: Kang Patrick T. , Yun June , Kaumaya Pravin P. T. , Chen Yeong‐Renn 
Published: June 2010

AbstractMitochondria are the major source of reactive oxygen species. Both complex I and complex II mediate O production in mitochondria and host reactive protein thiols. To explore the functions of the specific domains involved in the redox modifications of complexes I and II, various peptide‐based antibodies were generated against these complexes, and their inhibitory effects were subsequently measured. The redox domains involved in S‐glutathionylation and nitration, as well as the binding motif of the iron–sulfur cluster (N1a) of the complexes I and II were utilized to design B‐cell epitopes for generating antibodies. The effect of antibody binding on enzyme‐mediated O generation was measured by EPR spin trapping. Binding of either antibody AbGSCA206 or AbGSCB367 against glutathione (GS)‐binding domain to complex I inhibit its O generation, but does not affect electron transfer efficiency. Binding of antibody (Ab24N1a) against the binding motif of N1a to complex I modestly suppresses both O generation and electron transfer efficiency. Binding of either antibody Ab75 or Ab24 against nonredox domain decreases electron leakage for O production. In complex II, binding of antibody AbGSC90 against GS‐binding domain to complex II marginally decreases both O generation and electron transfer activity. Binding of antibody AbY142 to complex II against the nitrated domain modestly inhibits electron leakage, but does not affect the electron transfer activity of complex II. In conclusion, mediation of O generation by complexes I and II can be regulated by specific redox and nonredox domains. © 2010 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 96: 207–221, 2011.
Preparation of connexin43‐integrated giant Liposomes by a baculovirus expression–liposome fusion method
Authors: Kamiya Koki , Tsumoto Kanta , Arakawa Satoko , Shimizu Shigeomi , Morita Ikuo , Yoshimura Tetsuro , Akiyoshi Kazunari 
Published: June 2010

AbstractConnexin‐43 (Cx43) containing giant liposomes (GL) were prepared by a baculovirus expression–liposome fusion method. Recombinant budded viruses expressing Cx43 were prepared and then fused with GLs containing DOPG/DOPC at pH 4.5. Connexon formation on the GL membrane was observed by transmission electron microscope. Hydrophilic fluorescent dye transfers were observed through a Cx43‐mediated pathway not only between Sf9 (Spodoptera frugiperda) cells with Cx43 but also from giant Cx43 liposomes to Cx43‐expressing U2OS cells (human osteosarcoma cell). The functional connexin‐containing liposome is expected to be useful for cellular cytosolic delivery systems. The original orientation and function of Cx43 was maintained after integration into the liposomes. The liposome fusion method will create new opportunities as a tool for analysis of channel membrane proteins. Biotechnol. Bioeng. 2010;107: 836–843. © 2010 Wiley Periodicals, Inc.
The influence of oxygen supply on metabolism of neural cells cultured on a gas‐permeable PTFE foil
Authors: Mauth Corinna , Pavlica Sanja , Deiwick Andrea , Steffen Anja , Bader Augustinus 
Published: June 2010

AbstractThe influence of oxygen on neural stem cell proliferation, differentiation, and apoptosis is of great interest for regenerative therapies in neurodegenerative disorders, such as Parkinson's disease. These oxygen depending mechanisms have to been considered for the optimization of neural cell culture conditions. In this study, we used a cell culture system with an oxygen‐permeable polytetrafluorethylene (PTFE) foil to investigate the effect of oxygen on metabolism and survival of neural cell lines in vitro. Human glial astrocytoma‐derived cells (GOS‐3) and rat pheochromacytoma cells (PC12) were cultured on the gas‐permeable PTFE foil as well as a conventional non oxygen‐permeable cell culture substrate at various oxygen concentrations. Analyses of metabolic activity, gene expression of apoptotic grade, and dopamine synthesis were performed. Under low oxygen partial pressure (2%, 5%) the anaerobic metabolism and apoptotic rate of cultured cells is diminished on PTFE foil when compared with the conventional culture dishes. In contrast, under higher oxygen atmosphere (21%) the number of apoptotic cells on the PTFE foil was enhanced. This culture model demonstrates a suitable model for the improvement of oxygen dependent metabolism under low oxygen conditions as well as for induction of oxidative stress by high oxygen atmosphere without supplementation of neurotoxins. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Technologies and methods in virtual campus for improving learning process
Authors: Kybartaite A. , Nousiainen J. , Malmivuo J. 
Published: June 2010

AbstractThis paper presents technologies and methods that have been used when developing a virtual campus for Biomedical Engineering. Modern educational technologies have been applied to produce video lectures for personal computers, iPods, media phones, and to integrate virtual communication system. It became possible to break traditional classroom boundaries and to develop a worldwide, low‐cost, modern technology‐based virtual campus. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 21: 185–192, 2013
Practices of advanced programming: Tradition versus innovation
Authors: Fernández Leiva Antonio J. , Civila Salas Amparo C. 
Published: June 2010

AbstractThis paper compares two different approaches for teaching complex programming concepts in lab sessions. The first approach is based on the widely held assumption that the programming knowledge and skills which the student obtains are predominantly acquired from classroom activities undertaken at the instigation of the teacher. This automatically implies that the student must attend a minimum number of lab sessions. The second approach is to motivate student learning via a full‐term activity based on the implementation of computer games from scratch. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 21: 237–244, 2013
The ICT skills gap in Spain: Industry expectations versus university preparation
Authors: Llorens Ariadna , Llinàs‐Audet Xavier , Ras Antoni , Chiaramonte Luís 
Published: June 2010

AbstractThis article distinguishes which generic skills are the key to enable newly graduated engineers, basically computer and telecommunication engineers, to enter the information and communication technology (ICT) sector, satisfying industry demand in Spain. It also demonstrates significant differences between the engineering degrees (at graduate level) and the engineering master degrees (at post‐graduate level) related to skills needs. Furthermore, this work establishes the fact that there is a gap between the expectations of the ICT sector and the computer‐based engineering curricula and telecommunication engineering curricula the graduated receives, and where this gap appears in relation to generic competences or skills. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 21: 256–264, 2013
ChemInform Abstract: Synthesis of Novel Restricted Diamines: 2‐(1‐Aminocycloalkan‐1‐yl)ethylamines.
Authors: SUZUKI T. , IMANISHI N. , ITAHANA H. , WATANUKI S. , OHTA M. , MASE T. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: K6Sn23Bi2 (I) and K6Sn25 (II) — Two Phases with Chiral Clathrate Structure and Their Reactivity Towards Ethylenediamine.
Authors: FAESSLER T. F. , KRONSEDER C. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Anellation to the Quinazoline Ring. Preparation and Utilization of 3‐Quinazolylcarboxylates in the Syntheses of Fused Systems.
Authors: SPIRKOVA K. , STANKOVSKY S. , ZAHRADNIK M. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Hydrodimerization of Cyclic α,β Unsaturated Ketones Promoted by Samarium Iodide.
Authors: CABRERA A. , ROSAS N. , SHARMA P. , LE LAGADEC R. , VELASCO L. , SALMON M. , ARIAS J. L. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Potent HIV Protease Inhibitors Incorporating High‐Affinity P2‐Ligands and (R)‐(Hydroxyethylamino)sulfonamide Isostere.
Authors: GHOSH A. K. , ET AL. ET AL. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Dihydropyrancarboxamides Related to Zanamivir: A New Series of Inhibitors of Influenza Virus Sialidases. Part 1. Discovery, Synthesis, Biological Activity, and Structure—Activity Relationships of 4‐Guanidino‐ and 4‐Amino‐4H‐pyran‐6‐carboxamides.
Authors: SMITH P. W. , ET AL. ET AL. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Thieno[b]quinolizidines and Hofmann Elimination on Methiodides Derivatives.
Authors: BERKES D. , NETCHITAILO P. , MOREL J. , DECROIX B. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: α‐(Arylthio)imidoyl Radicals: [3 + 2] Radical Annulation of Aryl Isothiocyanates with 2‐Cyano‐Substituted Aryl Radicals.
Authors: LEARDINI R. , NANNI D. , PARESCHI P. , TUNDO A. , ZANARDI G. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthetic Transformation of Abietic Acid. Part 1. Addition of Dienophiles.
Authors: HASLINGER E. , HOFNER D. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Polysaccharide‐Based Chiral LC Columns
Authors: YASHIMA E. , YAMAMOTO C. , OKAMOTO Y. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Pentatomic Cyclic Antagonists and Muscarinic Receptors: A 30‐Year Review
Authors: ANGELI P. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Crystal Structure of Bi2Te2O7.
Authors: MERCURIO D. , CHAMPARDNAUD‐MESJARD J. C. , GOUBY I. , FRIT B. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Crystal Structures of Phenyltrimethylammonium Salts of Tetrabromoselenate(II) Bromide, [C6H5(CH3)3N]2 [SeBr4]× [C6H5(CH3)3N]Br and a Mixed Tetra(bromo/chloro)selenate(II).
Authors: HAUGE S. , JANICKIS V. , MAROEY K. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and Crystal Structures of Phenyltrimethylammonium Salts of Hexabromotellurate(IV), [C6H5(CH3)3N]2 [TeBr6], and catena‐poly [(Di‐μ‐bromobis{tetrabromotellurate(IV)})‐μ‐bromine], [C6H5(CH3)3N]2n [Te2Br10×Br2]n.
Authors: HAUGE S. , MAROY K. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Axially Chiral Cyclopentadienyl Ligands: Stereoselective Synthesis of 1‐Substituted‐9‐(1′‐naphthyl)fluorenes and Retention of Axial Chirality in the Fluorenyl Carbanions.
Authors: BAKER R. W. , FOULKES M. A. , TAYLOR J. A. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Carbon—Carbon Bond Cleavage of α‐Substituted Benzoins by Retro‐Benzoin Condensation; a New Method of Synthesizing Ketones.
Authors: MIYASHITA A. , SUZUKI Y. , OKUMURA Y. , IWAMOTO K. , HIGASHINO T. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The Preparation of Heterocyclic Appended Vinylogous Iminium Salts and Their Application to the Regioselective Preparation of Biheterocyclic Systems.
Authors: GUPTON J. T. , PETRICH S. A. , HICKS F. A. , WILKINSON D. R. , VARGAS M. , HOSEIN K. N. , SIKORSKI J. A. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Mild Method for Introducing Iodine Monofluoride into Alkenes and Iodination of Aromatics Using Xenon Difluoride.
Authors: SHELLHAMER D. F. , JONES B. C. , PETTUS B. J. , PETTUS T. L. , STRINGER J. M. , HEASLEY V. L. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of 1,2‐Amino Alcohols by Ti(IV)‐Catalyzed Photohydroxymethylation of Chiral Aldimines.
Authors: GRIESBECK A. G. , BUHR S. , LEX J. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: One‐Step Synthesis and Pharmacological Activity of New (N‐Substituted)amino‐spiroalkan‐dione Derivatives.
Authors: BONSIGNORE L. , LOY G. , COTTIGLIA F. , CALIGNANO A. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.