bluuebunny/crossref_metadata_2025_split huggingface dataset:
METHYL CELLULOSE ENHANCE GELATIN MEMBRANE AS GUIDANCE CHANNELS FOR PERIPHERAL NERVE REGENERATION
Authors: Chang Kuo-Chi , Tang Tzu-Piao , Hsu Chung-King , Soung Hung-Sheng , Wang Mao-Hsien 
Published: January 2012

Background: The methyl cellulose could successfully be transferred into 2,3-dialdehyde cellulose by using sodium periodate as an oxidant. 2,3-dialdehyde cellulose can cross-link gelatin without adding any other cross-linking agent. We hope the dialdehyde cellulose-gelatin membrane could enhance the physical properties of gelatin membrane by using glutaraldehyde as a cross-linker. Methods: The methyl cellulose could be successfully transferred into 2,3-dialdehyde cellulose by using sodium periodate as an oxidant. The biological stability of 2,3-dialdehyde cellulose was improved by cross-linking with gelatin. To estimate the properties of the DAC-Gel membrane with different conditions, the methyl cellulose was oxidized by different concentration of sodium periodate. After oxidized, the dialdehyde cellulose with different degree of oxidation was fabricated. FTIR could further confirm the formation of aldehyde group and the quantification of aldehyde group showed that the concentration of sodium periodate was the dominant factor to the degree of oxidation. Assessment of cross-linking degree helped us to observe the reaction between aldehyde group and amine group. The degradation test was estimated by two methods to confirm the accuracy of experiment. The hydrophilic/hydrophobic surface after cross-linking was evaluated by water contact angle test. The wettability of membrane influenced the growth of PC-12 cell. After the physical properties of membrane were established, the bio-compatibility was measured by two different methods. One of the methods based on ISO 10993. The other method is that cell seeding on the membrane. The bio-compatibility was evaluated by WST-1 and LDH. Result: Methyl cellulose was successfully converted into 2,3-dialdehyde cellulose by sodium periodate and the concentration of oxidant was the most important factor to the formation of aldehyde group. The formation of aldehyde group was directly proportional to the concentration of oxidant. Basic assessment of cross-linking degree showed that steric hindrance could be the factor to hinder cross-linking. The 2,3-dialdehyde cellulose/gelatin membrane could prolong the degradation time in 37°C. According to water contact test, the dialdehyde cellulose/gelatin membrane showed a more hydrophilic than gelatin membrane using glutaraldehyde as a cross-linker and the hydrophilic surface provided a better place for the growth of PC-12 cell. The dialdehyde cellulose/gelatin membrane using glycine to block the residual aldehyde group showed a low cytotoxicity. Conclusion: This study indicates that 2,3-dialdehyde cellulose/gelatin membrane could prolong the degradation time in 37°C and the 2,3-dialdehyde cellulose/gelatin membrane provide a better place for the growth of PC-12.
Human NK Cells Induce Neutrophil Apoptosis via an NKp46- and Fas-Dependent Mechanism
Authors: Thorén Fredrik B. , Riise Rebecca E. , Ousbäck Jenny , Della Chiesa Mariella , Alsterholm Mikael , Marcenaro Emanuela , Pesce Silvia , Prato Carola , Cantoni Claudia , Bylund Johan , Moretta Lorenzo , Moretta Alessandro 
Published: January 2012

Abstract
Polymorphonuclear neutrophils (PMN) are potent inflammatory effector cells essential to host defense, but at the same time they may cause significant tissue damage. Thus, timely induction of neutrophil apoptosis is crucial to avoid tissue damage and induce resolution of inflammation. NK cells have been reported to influence innate and adaptive immune responses by multiple mechanisms including cytotoxicity against other immune cells. In this study, we analyzed the effect of the interaction between NK cells and neutrophils. Coculture experiments revealed that human NK cells could trigger caspase-dependent neutrophil apoptosis in vitro. This event was dependent on cell–cell contact, and experiments using blocking Abs indicated that the effect was mediated by the activating NK cell receptor NKp46 and the Fas pathway. CD56-depleted lymphocytes had minimal effects on neutrophil survival, suggesting that the ability to induce neutrophil apoptosis is specific to NK cells. Our findings provide evidence that NK cells may accelerate neutrophil apoptosis, and that this interaction may be involved in the resolution of acute inflammation.
Totally Implantable Central Venous Access Ports in Patients with Cystic Fibrosis: A Multicenter Prospective Cohort Study
Authors: Dal Molin Alberto , Di Massimo Dino Stefano , Braggion Cesare , Bisogni Sofia , Rizzi Elena , D'Orazio Ciro , Di Toppa Maria Vittoria , Alghisi Federico , Cristadoro Simona , Carnovale Vincenzo , Festa Gabriella , Rampini Serena , Colombo Carla , Oneta Anna , Furnari Maria Lucia , Calamia Maria Antonietta , Zunino Maria Luisa , Tuccio Giuseppe , Spadea Vincenzo , Messore Barbara , Grosso Bianca , Festini Filippo 
Published: January 2012

Purpose The aim of this study was to assess the incidence of late onset complications of totally implantable venous access devices (TIVAD) in patients with cystic fibrosis (CF) and to investigate possible associations between the rate of complications and different policies of TIVAD management. Methods A multicenter prospective cohort study was performed in 11 Italian CF Centers. Patients with CF and a TIVAD were recruited and followed-up. Results The study commenced on May 2008 and ended on September 2010. Eighty subjects were studied (77.5% women - mean age 27.2 years). Eighteen late complications of ports were observed (22.5%; incidence 0.96 per 1000 days of observation): three lumen occlusions, seven catheter-related infections, three port-related venous thrombosis, in addition to five other complications. A statistically significant association was found between the onset of catheter-related infection and the presence of CF-related diabetes (CFRD) (P=.0064) Conclusions Our data suggest that TIVADs represent a safe and effective device for the intermittent IV administration of drugs in people with CF. However, people with CFRD have a higher risk of developing TIVAD-related infection.
Retrograde popliteal access as bail‐out strategy for challenging occlusions of the superficial femoral artery: A multicenter registry
Authors: Sangiorgi Giuseppe , Lauria Giulia , Airoldi Flavio , Godino Cosmo , Politi Luigi , Colombo Antonio , Clerici Giacomo , Modena Maria Grazia , Biondi‐Zoccai Giuseppe 
Published: January 2012

AbstractBackground: The concomitant use of femoral and popliteal accesses has been recommended for challenging superficial femoral artery (SFA) occlusions, but no comprehensive comparison of this approach to a strategy of femoral access only is available. We thus aimed to appraise the risk‐benefit balance of retrograde popliteal access as bail‐out strategy for SFA occlusions. Methods: Consecutive patients with symptomatic SFA occlusion and undergoing percutaneous revascularization were enrolled. We distinguished patients in whom retrograde popliteal access was required as bail‐out strategy versus those not requiring such access. The primary end‐point was procedural success. Results: A total of 130 patients (152 limbs) were included, with 23 patients (25 limbs) requiring retrograde popliteal access. Occlusion length was 20.6 ± 8.8 cm in those requiring popliteal access versus 18.5 ± 8.5 cm in those without popliteal access, with TASC C/D lesions in 23 (92%) versus 106 (83%). Procedural success was achieved in 92 out of 107 patients (86.0%) treated with a standard approach and 22 out of 23 patients (95.7%) treated with retrograde popliteal access (total 114 out of 130 [87.7%]) and 112 out of 127 limbs (88.2%) and 24 out of 25 limbs (96.0%), respectively (total 136 out of 152 [89.5%]). No significant increase in early or long‐term adverse events was associated with retrograde popliteal access. Conclusions: Whenever standard access sites do not enable successful recanalization of SFA occlusions, retrograde popliteal access can be safely and effectively envisioned as bail‐out strategy. © 2012 Wiley Periodicals, Inc.
Transient kinetics of n‐butane partial oxidation at elevated pressure
Authors: Shekari Ali , Patience Gregory S. 
Published: January 2012

AbstractA transient Mars‐van Krevelen type kinetic model was developed for n‐butane partial oxidation over vanadyl pyrophosphate (VPP) catalyst. The model validity was verified over a relatively wide range of redox feed compositions as well as higher reactor pressure (410 kPa). Oxygen and n‐butane conversion increased with higher pressure while maleic anhydride (MA) selectivity decreased by as much as 20%. However, the overall MA yield was enhanced by up to 30%. High pressure maintains the catalyst in a higher oxidation state (as long as there is sufficient oxygen in the gas phase) and as a consequence, the catalytic activity is improved together with MA yield. High pressure also affects the redox reaction rates and activation energies. © 2012 Canadian Society for Chemical Engineering
A nonlinear filtered‐x prediction error method algorithm for digital predistortion in digital subscriber line systems
Authors: Abd‐Elrady Emad , Gan Li , Kubin Gernot 
Published: January 2012

SUMMARYDigital predistortion of nonlinear systems is an important topic in many practical applications. This paper considers direct predistortion of a Volterra system by connecting in tandem an adaptive Volterra predistorter. The coefficients of the predistorter can be recursively estimated using the nonlinear filtered‐x least mean squares (NFxLMS) algorithm. In this paper, the prediction error method (PEM) is used to derive a novel nonlinear filtered‐x PEM (NFxPEM) algorithm. A simulation study on Volterra systems shows that the NFxPEM algorithm more significantly suppresses spectral regrowth and converges much faster than the NFxLMS algorithm. Also, the NFxPEM algorithm is used in this paper to design more efficient digital predistorter—as compared with the NFxLMS algorithm—for digital subscriber line systems. Copyright © 2012 John Wiley & Sons, Ltd.
Summary of ‘Mass media interventions for preventing smoking in young people’
Authors: Editorial Office Evidence‐Based Child Health 
Published: January 2012

AbstractThis is a summary of Cochrane review, published in this issue of EBCH, first published as: Brinn MP, Carson KV, Esterman AJ, Chang AB, Smith BJ. Mass media interventions for preventing smoking in young people. Cochrane Database of Systematic Reviews 2010, Issue 11. Art. No.: CD001006. DOI: 10.1002/ 14651858.CD001006.pub2. Copyright © 2011 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. The Cochrane Collaboration
Summary of ‘Adjusting the pH of lidocaine for reducing pain on injection’
Authors: Editorial Office Evidence‐Based Child Health 
Published: January 2012

AbstractThis is a summary of Cochrane review, published in this issue of EBCH, first published as: Cepeda MS, Tzortzopoulou A, Thackrey M, Hudcova J, Arora Gandhi P, Schumann R. Adjusting the pH of lidocaine for reducing pain on injection. Cochrane Database of Systematic Reviews 2010, Issue 12. Art. No.: CD006581. DOI: 10.1002/14651858.CD006581.pub2. Copyright © 2011 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. The Cochrane Collaboration
Cochrane Review: Care delivery and self‐management strategies for children with epilepsy
Authors: Lindsay Bruce , Bradley Peter M 
Published: January 2012

AbstractBackgroundEpilepsy care for children has been criticised for its lack of impact. Various service models and strategies have been developed in response to perceived inadequacies in care provision for children and their families.ObjectivesWe set out to compare the effectiveness of specialist or dedicated teams or individuals in the care of children with epilepsy with usual care services.Search methodsWe searched the Cochrane Central Register of Controlled Trials (CENTRAL) (The Cochrane Library February issue, 2010), MEDLINE (1950 to March 2010), EMBASE (1988 to May 2006*), PsycINFO (1806 to March 2010) and CINAHL (1982 to March 2010).*Please note that as we currently do not have access to EMBASE, we have been unable to update this aspect of our searching.Selection criteriaWe included randomised controlled trials, controlled or matched trials, cohort studies or other prospective studies with a control group, or time series studies.Data collection and analysisEach review author independently selected studies, extracted data and assessed the quality of included studies.Main resultsFour trials and five reports are included in this review. They report on four different education and counselling programmes for children, children and parents, or teenagers and parents. Each programme showed some benefits for the well‐being of children with epilepsy, but each trial had methodological flaws and no single programme was evaluated by more than one study.Authors' conclusionsWhile each of the programmes in this review showed some benefit to children with epilepsy their impacts were extremely variable. No programme showed benefits across the full range of outcomes. No study appears to have demonstrated any detrimental effects but the evidence in favour of any single programme is insufficient to make it possible to recommend one programme rather than another. More trials, carried out by independent research teams, are needed.Plain Language SummaryCare delivery and self‐management strategies for children with epilepsyEvidence for the best ways to care for children with epilepsy is still unclear.This review compared four education‐ or counselling‐based interventions for children with epilepsy. One intervention was aimed solely at children, two were aimed at children and their parents, the final intervention was aimed at teenagers and their parents. Each of the interventions appeared to improve some of the outcomes studied, but no intervention improved all of the outcomes that were measured. The studies also had problems with their methods, which makes their results less reliable. While none of the interventions caused any harm, their impact was limited and we cannot recommend any single intervention as being the best one for children with epilepsy.
Summary of ‘Nonsteroidal anti‐inflammatory drugs and perioperative bleeding in paediatric tonsillectomy’
Authors: Editorial Office Evidence‐Based Child Health 
Published: January 2012

AbstractThis is a summary of Cochrane review, published in this issue of EBCH, first published as: Cardwell ME, Siviter G, Smith AF. Nonsteroidal anti‐inflammatory drugs and perioperative bleeding in paediatric tonsillectomy. Cochrane Database of Systematic Reviews 2005, Issue 2. Art. No.: CD003591. DOI: 10.1002/14 651 858.CD003591.pub2. Copyright © 2011 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. The Cochrane Collaboration
Asymptotic performance of amplify‐and‐forward cooperative diversity networks with the Nth best relay over Rician fading channels
Authors: Chu Shao‐I , Chang Hsin‐Chiu , Lee Hung‐Peng 
Published: January 2012

ABSTRACTThis paper analyzes and derives the asymptotic symbol error rate expression of amplify‐and‐forward cooperative communications with the Nth best relay over independent and non‐identical Rician fading channels. Rician fading takes the line‐of‐sight effects into account and often exists in microcellular mobile and wireless mesh networks. In practice, the Nth best relay is selected when the best relay is unavailable because of fairness and load balancing issues. Performance analysis reveals that the diversity gain of the Nth best‐relay scheme equals R–N+2, while the best relay and all‐participate schemes achieve the diversity gain of R+1, where R is the number of relays. Simulation and numerical evaluation show the tightness of the asymptotic symbol error rate expression and the accuracy of our theoretical analysis. Copyright © 2012 John Wiley & Sons, Ltd.
Seeing touch and pain in a stranger modulates the cortical responses elicited by somatosensory but not auditory stimulation
Authors: Valentini Elia , Liang Meng , Aglioti Salvatore Maria , Iannetti Gian Domenico 
Published: January 2012

AbstractViewing other's pain inhibits the excitability of the motor cortex and also modulates the neural activity elicited by a concomitantly delivered nociceptive somatosensory stimulus. As the neural activity elicited by a transient nociceptive stimulus largely reflects non nociceptive‐specific, multimodal neural processes, here we tested, for the first time, whether the observation of other's pain preferentially affects the brain responses elicited by nociceptive stimulation, or instead similarly modulates those elicited by stimuli belonging to a different sensory modality. Using 58‐channel electroencephalography (EEG), we recorded the cortical responses elicited by laser and auditory stimulation during the observation of videoclips showing either noxious or non‐noxious stimulation of a stranger's hand. We found that the observation of other's pain modulated the cortical activity consisting in an event‐related desynchronization in the β band (β ERD), and elicited by nociceptive laser stimuli, but not by auditory stimuli. Using three different source analysis approaches, we provide converging evidence that such modulation affected neural activity in the contralateral primary sensorimotor cortex. The magnitude of this modulation correlated well with a subjective measure of similarity between the model's hand and the onlooker's representation of the hand. Altogether, these findings demonstrate that the observation of other's pain modulates, in a somatosensory‐specific fashion, the cortical responses elicited by nociceptive stimuli in the sensorimotor cortex contralateral to the stimulated hand. Hum Brain Mapp, 2012. © 2012 Wiley Periodicals, Inc.
Three‐dimensional epithelial and mesenchymal cell co‐cultures form early tooth epithelium invagination‐like structures: Expression patterns of relevant molecules
Authors: Xiao Li , Tsutsui Takeki 
Published: January 2012

AbstractEpithelium invagination is the key feature of early tooth development. In this study, we built a three‐dimensional (3D) model to represent epithelium invagination‐like structure by tissue engineering. Human normal oral epithelial cells (OECs) and dental pulp stem cells (DPSCs) were co‐cultivated for 2–7 weeks on matrigel or collagen gel to form epithelial and mesenchymal tissues. The histological change and gene expression were analyzed by HE staining, immunostaining, and quantitative real‐time RT‐PCR (qRT‐PCR). After 4 weeks of cultivation, OECs‐formed epithelium invaginated into DPSCs‐derived mesenchyme on both matrigel and collagen gel. OEC–DPSC co‐cultures on matrigel showed typical invagination of epithelial cells and condensation of the underlying mesenchymal cells. Epithelial invagination‐related molecules, CD44 and E‐cadherin, and mesenchymal condensation involved molecules, N‐cadherin and Msx1 expressed at a high level in the tissue model, suggesting the epithelial invagination is functional. However, when OECs and DPSCs were co‐cultivated on collagen gel; the invaginated epithelium was transformed to several epithelial colonies inside the mesenchyme after long culture period. When DPSCs were co‐cultivated with immortalized human OECs NDUSD‐1, all of the above‐mentioned features were not presented. Immunohistological staining and qRT‐PCR analysis showed that p75, BMP2, Shh, Wnt10b, E‐cadherin, N‐cadherin, Msx1, and Pax9 are involved in initiating epithelium invagination and epithelial–mesenchymal interaction in the 3D OEC–DPSC co‐cultures. Our results suggest that co‐cultivated OECs and DPSCs on matrigel under certain conditions can build an epithelium invagination‐like model. This model might be explored as a potential research tool for epithelial–mesenchymal interaction and tooth regeneration. J. Cell. Biochem. 113: 1875–1885, 2012. © 2012 Wiley Periodicals, Inc.
Effects of medium calcium, and agents affecting cytoskeletal function, on cellular volume and morphology in liver tissue in vitro
Authors: Russo Matteo A. , Morgante Emanuela , Tafani Marco , van Rossum George D.V. 
Published: January 2012

AbstractThe possible role of an exocytotic, vesicular mechanism in cellular volume regulation under iso‐osmotic conditions has been studied in slices of rat liver. The effects of incubation conditions and agents affecting the actin cytoskeleton were examined for changes of water, ionic composition, and ultrastructure. Slices were pre‐incubated at 1°C in an iso‐osmotic buffered medium to induce swelling. Upon restoration to 37°C in the same medium, tissue lost water. The Na+–K+ adenosine triphosphatase (ATPase) inhibitor ouabain inhibited water extrusion of about 50%, an effect that was accompanied by the formation of characteristic vesicles in the cytoplasmic region between the Golgi apparatus and the bile canaliculi. Water extrusion in the presence of ouabain was partially inhibited by trifluoroperazine and completely inhibited when the medium was free of Ca2+. In the presence of ouabain, brefeldin A caused a small reduction of water extrusion, whereas phalloidin and cytochalasins A, D, or E caused a marked inhibition. In these conditions there was a marked increase in size and number of cytoplasmic vesicles and a more widespread distribution of them within the cells, lacking the more specific orientation to the Golgi and canalicular regions that was seen in the presence of ouabain alone. Water extrusion was inhibited by phalloidin and cytochalasins in the absence of ouabain. In conclusion, our results are consistent with the hypothesis that iso‐osmotic expulsion of water from hepatocytes can proceed partly through an accumulation of water in cytoplasmic vesicles, followed by exocytosis. This mechanism does not depend on Na+–K+ ATPase activity. J. Cell. Biochem. 113: 1915–1925, 2012. © 2012 Wiley Periodicals, Inc.
Removal of oxytetracycline (OTC) in a synthetic pharmaceutical wastewater by sequential anaerobic multichamber bed reactor (AMCBR)/completely stirred tank reactor (CSTR) system: biodegradation and inhibition kinetics
Authors: Sponza Delia Teresa , Çelebi Hakan 
Published: January 2012

AbstractBACKGROUND: The antibiotics in industrial and munipical wastewaters could not be removed effectively in conventional anaerobic and aerobic biological treatment plants. Few studies have been performed to investigate the biodegradation and inhibition kinetics of oxytetracycline (OTC) on methanogens and total volatile fatty acids (TVFA).RESULTS: A high rate anaerobic multichamber bed reactor (AMCBR) was effective in removing the molasses‐chemical oxygen demand (COD), and the OTC antibiotic with yields as high as 96% at an influent OTC loading rate of 133.33 gOTC m−3 day−1 at a hydraulic retention time (HRT) of 2.25 days. Increasing the OTC loading rates from 22.22 gOTC m−3 day−1 to 133.33 gOTC m−3 day−1 improved both hydrolysis and specific utilization of molasses‐COD. The inhibition constants of TVFA (KI−TVFA−meth) and OTC (KI−OTC−meth) on methanogens decreased at OTC loadings > 133.33 gOTC m−3 day−1. The direct effect of OTC loadings > 133.3 gOTC m−3 day−1 on acidogens and methanogens was evaluated using the Haldane inhibition kinetic.CONCLUSION: OTC antibiotic was effectively removed in a sequential AMCBR/completely stirred tank reactor (CSTR). The Haldane inhibition constant (KID) decreased significantly at high OTC loads indicating the increase in toxicity. Copyright © 2012 Society of Chemical Industry