bluuebunny/crossref_metadata_2025_split huggingface dataset:
CARM1 inhibitor TP064 attenuates endothelial cell dysfunction via inhibits inflammatory response in vitro model of subarachnoid hemorrhage
Authors: Zhang Qingtao , Zhang Ping , Liang Yidan , Yang Qiang , Xu Lei , Deng Yongbing , Cui Min , Zhou Weiduo , Sun Chao , Liu Liu , Wu Min 
Published: May 2024

Abstract

Cerebral endothelial cell dysfunction plays a critical role in the pathophysiology of vascular injury subsequent to subarachnoid hemorrhage (SAH), yet the precise molecular mechanism remains largely speculative. Inflammation stands out as a pivotal contributor to an unfavorable prognosis post-SAH, with nuclear factor-κB (NF-κB) pathways being initiated and ultimately leading to inflammation activation and pro-inflammatory cytokine release following SAH. In this study, we explored the impact of the Coactivator-associated arginine methyltransferase 1 (CARM1) inhibitor TP-064 on inflammation using an in vitro SAH model. Exposure of endothelial cells to TP-064 resulted in a significant reduction in CAMR1 and NF-κB expression upon hemoglobin exposure. Similarly, endothelial cells treated with TP-064 following hemoglobin incubation exhibited decreased expression levels of intercellular adhesion molecule-1 (ICAM1), myeloperoxidase (MPO), and cytokine production including interleukin-1β (IL-1β), interleukin-12 (IL-12), tumor necrosis factor-α (TNF-α) in response to hemoglobin exposure. Moreover, subsequent investigations demonstrated that CARM1 transcriptionally regulates NF-κB via methylation. Additionally, TP-064 notably mitigated endothelial dysfunction. Collectively, our findings identify TP-064 as a CARM1 inhibitor targeting inflammation and neutrophil infiltration, offering new insights into therapeutic strategies for addressing endothelial cell dysfunction following SAH.
Domesticated cannabinoid synthases amid a wild mosaic cannabis pangenome
Authors: Michael Todd , Lynch Ryan , Padgitt-Cobb Lillian , Garfinkel Andrea , Knaus Brian , Hartwick Nolan , Allsing Nicholas , Alyward Anthony , Mamerto Allen , Kitony Justine , Colt Kelly , Murray Emily , Duong Tiffany , Trippe Aaron , Crawford Seth , Vining Kelly 
Published: May 2024

Abstract

Cannabis sativa is a globally significant seed-oil, fiber, and drug-producing plant species. However, a century of prohibition has severely restricted legal breeding and germplasm resource development, leaving potential hemp-based nutritional and fiber applications unrealized. Existing cultivars are highly heterozygous and lack competitiveness in the overall fiber and grain markets, relegating hemp to less than 200,000 hectares globally1. The relaxation of drug laws in recent decades has generated widespread interest in expanding and reincorporating cannabis into agricultural systems, but progress has been impeded by the limited understanding of genomics and breeding potential. No studies to date have examined the genomic diversity and evolution of cannabis populations using haplotype-resolved, chromosome-scale assemblies from publicly available germplasm. Here we present a cannabis pangenome, constructed with 181 new and 12 previously released genomes from a total of 156 biological samples from both male (XY) and female (XX) plants, including 42 trio phased and 36 haplotype-resolved, chromosome-scale assemblies. We discovered widespread regions of the cannabis pangenome that are surprisingly diverse for a single species, with high levels of genetic and structural variation, and propose a novel population structure and hybridization history. Conversely, the cannabinoid synthase genes contain very low levels of diversity, despite being embedded within a variable region containing multiple pseudogenized paralogs and distinct transposable element arrangements. Additionally, we identified variants of acyl-lipid thioesterase (ALT) genes2 that are associated with fatty acid chain length variation and the production of the rare cannabinoids, tetrahydrocannabinol varin (THCV) and cannabidiol varin (CBDV). We conclude the Cannabis sativa gene pool has only been partially characterized, and that the existence of wild relatives in Asia remains likely, while its potential as a crop species remains largely unrealized.
WITHDRAWN: Cancer epidemiology in Pakistan
Authors: Rashid Muhammad Usman , Badar Farhana , Mahmood Shahid 
Published: May 2024

Abstract

Introduction: This research study provides valuable insights into the distribution of malignancies among different population groups, age groups, genders, and geographical regions.
Aim: This study aims to identify differences in cancer cases between adults and pediatric populations, analyze behavior distribution by gender and age group, and assess the prevalence of specific cancer types across different demographics. Methods: The study analyzed data from Shaukat Khanum Memorial Cancer Hospital and Research Center (SKMCH&RC) in 2022, examining cancer cases in adults and pediatric patients, behavior distribution, and analytical proportions. It assessed malignancies by gender and age group, identified top 10 prevalent cancers.
Results: The study found that among adult patients, 87.93% had malignant cancers, while in pediatric patients, it was 11.99%. Benign and uncertain cases were prevalent in adults (97.78%), with pediatric cases at 2.21%. Punjab and Khyber Pakhtunkhwa had high cancer rates compared to other provinces. The top 10 cancers included breast, colon, rectum & anus, Hodgkin lymphoma, lip & oral cavity, esophagus, prostate, non-Hodgkin's lymphoma, leukemia, kidney, and stomach cancer. Most cases were local or regional (23% and 44.3%, respectively), while distant cases were 20.5%, and 11.2% were unknown.
Conclusion: This research study provides insights into cancer distribution, guiding prevention and treatment strategies. It highlights the importance of targeted prevention, early detection, and accurate staging. Valuable for policymakers, health professionals, and researchers, advancing cancer control and patient outcomes.
Bridging Post Cholecystectomy CBD Transections through a Novel Drain loop Technique – How I do it !
Authors: Garg Parul , Sharma Malay 
Published: May 2024

Abstract

Complete transection of the common bile duct (CBD) is a complication of Cholecystectomy which can be difficult to repair by surgery and management by Endoscopic Retrograde Cholangiography is also difficult due to failure of negotiation of wire across the transected ends of the CBD. Patients usually present in a state of sepsis with bile leak and continuous output of bile from the surgical drains. After failure of ERCP, percutaneous approach can be tried by interventional Radiologist, however passing a guide wire across cut ends of CBD is difficult even by routine percutaneous approach. We describe a novel technique which we used in three patients of CBD transection to bridge the wire across the transected ends of CBD facilitating the placement of plastic stents across the duct transection. Our technique helps in rescuing patients from a state of sepsis and morbidity and can be an option to avoid the often-difficult surgery in cases of interruption of biliary tree. If required surgery can be performed later in elective conditions when patient’s general condition and technical availability of surgical expertise is feasible. “Level of Evidence: Level 5, Mechanism based reasoning.”
A spatially resolved human glioblastoma atlas reveals distinct cellular and molecular patterns of anatomical niches
Authors: Shah Nameeta , Park Hyun Jung , Sonpatki Pranali , Schroeder Brett , Han Kyung Yeon , Kim Hye Jin , Chowdhury Tamrin , Hwang Jong Ha , Nam Sun Mo , Byun Yoon Hwan , Kang Ho , Lee Joo Ho , Lee Soon-Tae , Won Jae-Kyung , Kim Tae Min , Choi Seung Hong , Ku Ja-Lok , Lee Sungyoung , Yun Hongseok , Park Sung-Hye , Park Chul-Kee , Park Woong-Yang 
Published: May 2024

Abstract

Glioblastoma (GBM) is a dynamic ecosystem with high plasticity where the complex interplay between different cellular components contributes to disease progression. Although single-cell RNA (scRNA)-seq has revealed remarkable cellular heterogeneity of GBM, the spatial organization of its diverse cell types is currently lacking. Here we present a comprehensive dataset of 115,914 spatial transcriptomes across 32 tissue sections of genotyped glioma samples. We present spatial maps of 56 fine-grained cellular components, including previously unrecognized oligodendrocyte subtypes and their interactive networks within each anatomical niche. Additionally, we utilized Xenium in situ technology to generate subcellular resolution spatial transcriptomic data, enhancing our understanding of GBM at a granular level. Our data provides novel insights into the cellular architecture of GBM. This valuable and openly shared resource will help develop new and effective combinatorial therapies to target multiple niches simultaneously to improve patient outcomes.
Exploding drops on lubricated surfaces
Authors: Lin Marcus , Zhang Peng , Daniel Dan 
Published: May 2024

Abstract

Traditionally, investigations of Coulomb explosions have focused on charged microdrops levitated using quadrupole electric fields, i.e., a Paul trap. In a surprising twist, our work introduces a simple method to observe Coulomb explosions, with no drop levitation and no external electric field. Instead, we generate a charged water drop using a conventional micropipette, which we then deposit on a plastic petri dish lubricated with a thin oil film. As the droplet evaporates, its radius shrinks until it reaches the Rayleigh limit at which point we observe multiple, highly periodic Coulomb explosions (> 60 events over 30 mins)—the first time Coulomb explosions have been reported for a sessile drop on a surface. Each event produces a finely ejected liquid jet, which disintegrate into microdroplets explosively within microseconds. Intriguingly, the application of a small electric field ∼ 103 V m−1, just larger than the atmospheric electric field, aligns the jetting direction. Our Coulomb explosions span diverse length scales (from micron to millimetres) and time scales (from microseconds to minutes), with potentially wide-ranging applications from nanoscopic material fabrication to electrospray ionization.
Investigating the Effects of Dampness and Water seepage on Structural Integrity of Buildings: A Case Study of Ransom Kuti Hall, University of Ibadan, Ibadan, Nigeria
Authors: Ojedele Jubril , Akinboade Temiloluwa , Ajagbe Abdulwasiu 
Published: May 2024

Abstract

Dampness and water seepage are among the leading moisture problems that are encountered in building structures, particularly public buildings such as the University of Ibadan hostel buildings, and these water problems in turn affect the structural integrity of the buildings. The aim of this study was to investigate the effects of water seepage and dampness on the structural integrity of buildings in Ransom Kuti Hall of Residence, University of Ibadan. This study was carried out by investigating the locations affected by water seepage and damping through visual assessments to determine the causes and effects of water seepage and damping on buildings. Consequently, the strength test uses the rebound hammer to obtain the residual strength of the affected structural elements in the buildings. The results showed that dampness and water seepage problems were caused by different factors, such as rainfall and ponding, faults in the plumbing units within the buildings, and sometimes, poor construction materials. This study identified a number of building defects that occurred due to dampness and water seepage problems, such as spalling, paint peeling and algae growth. Furthermore, the results of the strength test showed that there was an average percentage loss in strength of 8.86% in the area affected by dampness, 25.47% in the area affected by water seepage and 40.18% in the area that underwent spalling. These problems can be concluded to be serious causes of building defects. Ransome Kuti hall of residence can be categorized as an old building structure that has been in operation for more than 65 years, and these findings provide an interesting view of dampness and water seepage problems that affect the hostel functional performance.
Crystalography and the behavior of optical band gap in transition metal-doped (Al+Mn) ZnO nanopowder
Authors: Harsono Heru , Istiroyah  , Pamungkas Mauludi Ariesto , Febia Vonilu , Gaol Arivicki Lumban 
Published: May 2024

ZnO and Zn(0.97-x)Al0.03MnxO (x=0.00 (AlMnZ0), 0.04 (AlMnZ4), 0.05 (AlMnZ5) and 0.06 (AlMnZ6)) nanopowders that have been synthesized in powder form by using coprecipitation method at powder-based low temperature are zinc chloride (Sigma Aldrich), manganese (II) chloride tetrahydrate (Sigma Aldrich), and aluminum chloride hexahydrate (Sigma Aldrich).
The powder of zinc chloride, aluminum chloride hexahydrate and manganese (II) chloride are dissolved in 0.5 M HCl, then a precipitating agent is added in the form of NaOH 5 M base into the solution by slowly dropping NaOH 5 M solution into the precursor until a pH of 10 is reached which causes the clear and transparent solution turns into milky white.
In order for the reaction to run perfectly, the stirring process is carried out in a hot plate stirrer for 90 minutes at a temperature of 85 oC. After the solution is formed, it is washed with distilled water repeatedly until there is no impurities and it is filtered with filter paper to separate the precipitate from the solution. X-ray Diffraction (XRD) and Ultra Violet Visible (UV-VIS) Spectroscopy have been used to characterize ZnO nanopowder products through (Al+Mn) doping. The results show that AlMnZ0, AlMnZ4, AlMnz5 and AlMnZ6 nanopowder has the form of a hexagonal wurtzite crystal phase with a crystal size of ~38.03881–47.57561 nm. The optical band gap of ZnO nanopowder can be increased by atomic doping (Al+Mn). The increase in the optical band gap of AlMnZ0, AlMnZ4, AlMnZ5 and AlMnZ6 nanopowder is related to the Burstein-Moss effect, that is when (Al+Mn) atoms are successfully incorporated into ZnO nanopowders, there is a vacancy of the oxygen atom in the host crystal
Improvement of chainsaw sounds identification in the forest environment using maximum ratio combining and classification algorithme
Authors: Gnamele N’tcho Assoukpou Jean , Youan Bi Tra Jean Claude , Famien Adjoua Moise Landry 
Published: May 2024

To better combat the devastation of the protected forests in Côte d’Ivoire, a study was conducted to create a technique for detecting the acoustic signals produced by chainsaws deployed to fell trees in these areas. To improve the recognition rate of chainsaw sounds in a forest environment and increase the detection range of the recognition system, we are implementing the maximum ratio combining (MRC) technique on a microphone array.
Therefore, the employment of an identification system is compared using one (01) microphone against the outcomes obtained by adopting system with three (03), six (06), and twelve (12) microphones. The use of MRC is then contrasted with an alternative recombining approach, referred to as simple summation (SS). The SS is characterized by the mere addition of signals acquired by the network in the frequency domain.
The MRC was employed on various microphone arrangements, accounting for varying degrees of attenuation experienced by chainsaw sounds. The K-Nearest Neighbors, in combination with Mel Frequency Cepstral Coefficients (MFCC), was employed to detect chainsaw sounds within the 16 kHz central frequency octave band.
MRC applied to microphone arrays provided superior outcomes than simple summation. The enhancement in terms of classification rate ranged from [18; 51], favouring MRC. Moreover, it extended the chainsaw detection range from 520 m (using one microphone) to 1210 m (using a 12-microphone array).
Taking into account the criteria for selecting an optimum microphone array, including classification rate, number of microphone nodes, information processing time and detection range, the six-microphone array was chosen as the best configuration. This configuration boasts a theoretical detection range of 1040 meters
Application of digital simulation tool in designing of injection mold for sustainable manufacturing
Authors: Trinh Van-Long 
Published: May 2024

Gate location (GL) is one of the most important criteria in the injection molding technology to produce a good quality product. The best GL will bring many benefits to the manufacturing process such as reducing cycle time, minimizing defects of the product, and decreasing capital cost. In this study, an automatic gate location analysis tool (AGLA) is introduced to investigate the best gate location for designing the injection mold. EasyFill Advanced software was used to conduct experiments on analyzing the filling process of melting plastic material into the mold cavity. Acrylonitrile butadiene styrene (ABS) is the polymer material that is used in the study to produce the plastic product. The gate locations are located in different positions to analyze the product characteristics that will occur during the injection molding process such as weld lines, short shot, warpage, shrinkage, and air traps. Three simulation cases were set up and conducted including case 1 with the suggestion from EasyFill software, case 2 with the gate location located on the middle point of the long edge side, and case 3 with the gate location located on the middle point of the short edge side. The warpage defects are recorded with a minimum value of 0.464 mm belonging to case 1 smaller than that of 0.481 mm in case 2 and 0.482 mm in case 3, respectively. The method is a good solution for engineers in designing and developing a new mold for injection molding manufacturing
Recent Advancement of Nanotheranostics in Cancer Applications
Authors: Parveen Suphiya , Abira R , Paikray Safal , Sahoo Liza , Tripathy Nigam Sekahr , Dilnawaz Fahima 
Published: May 2024

Abstract:
The field of nanomedicine shows promising implications in the concurrent delivery of
therapeutic and diagnostic (theranostics) compounds in a single platform. Nanotheranostics is incredibly
promising since it offers simultaneous non-invasive disease detection and treatment together
with the exciting ability to track drug release and distribution in real-time, thereby forecasting
and evaluating the efficacy of the therapy. The cancer theranostic approach improves the cancer
prognosis safely and effectively. Common classes of nanoscale biomaterials, including magnetic
nanoparticles, quantum dots, upconversion nanoparticles, mesoporous silica nanoparticles, carbon-
based nanoparticles, and organic dye-based nanoparticles, have demonstrated enormous potential
for theranostic activity. The need for improved disease detection and enhanced chemotherapeutic
treatments, together with realistic considerations for clinically translatable nanomaterials will
be key driving factors for theranostic agent research shortly. The developments of precision theranostic
nanomaterials are employed in imaging systems like, MRI, PET, and SPECT with multifunctional
ability. In this review, different nanoparticles/nanomaterials that are used/developed for
theranostic activity are discussed.
The innovative methodology for teaching and learningabout sexuality care in a population of young Mexicans
Authors: Hernández Ortega Yolanda , Rico González María de Lourdes , García Maria de Lourdes , Varela Elena 
Published: May 2024

Introduction. Sex education in Mexico focuses on biological aspects, with traditional teaching-learning strategies, which do not encourage reflection on learning that impacts the care of sexuality, so it is urgent to propose a change so that the student becomes a subject and not an object of your learning. Objective. To report the experiences in developing the innovative methodology through the Maguerez Arch for the teaching and learning ofthe Care of the sexuality of the University Emerging Adult. Method. Qualitative study based on the Arco de Maguerez problematization methodology, developed on 12 Nursing students from 18 to 25 who attended 12 program sessions: “Taking care of my sexuality.” The reports were analyzed by content analysis and technique of the speech. Results. Ac-cording to the five moments of the Maguerez Arch: 1) Observation of reality: ignorance of contraceptive methods and sexually transmitted infections, 2) Identify the key points: An approach to sexuality to the biological aspect and non-invasive care it is part of their sexual life 3) Theorizing the problem: topics to be developed by the students: sexual education, communication, self-esteem, and empowerment, 4) Solution hypothesis: the care and knowledge of their sexuality if it is a priority in their life, 5 ) Reality application: they recognize the right to live sexuality with responsibility and care. Conclusions. The methodology was innovative, motivating, and significant, allowing the student to make conscious decisions regarding the care of their sexuality.
Investigating Factors Shaping Sunnah-Based Product Consumption among University Students
Authors: Anuar Annurizal , Othman Abdul Kadir , Maon Siti Noorsuriani , Abdullah Mohd Zulkifli , Dzulkipli Mohd Redhuan , Mat Hasan Noor Zaihan 
Published: May 2024

Health problems have increased because of modern lives, especially in young people because of poor nutrition and improper eating habits. Inspired by Islamic teachings, especially the Quran and the Sunnah of the Prophet Muhammad (PBUH), this study attempts to investigate the factors impacting Sunnah-based product consumption among Muslim college students. Data were gathered by utilizing a correlational research approach and questionnaires given to 476 Muslim university students. According to the study, 96.6% of respondents were aware of Sunnah-based products, and a sizable majority (93.9%) favored them. Childhood exposure to such products (44.3%) points to familial or cultural impacts. The frequency of consumption varied, though, with 41.8% consuming Sunnah-based goods on occasion. Taste concerns were negligible (12.0%) and accessibility issues (23.9%) emerged as the main obstacles to consumption, followed by high pricing (56.5%). Positive sentiments notwithstanding, real-world barriers impede widespread adoption. These results highlight how critical it is to remove obstacles preventing Muslim youth from using Sunnah-based products. Interventions can be designed to encourage healthy eating habits in line with Islamic teachings by knowing the factors influencing their decisions. This will ultimately improve the general health and well-being of Muslim communities.
Helping Your Students Overcome Cramming Using an Adapted Version of W. Edwards Deming’s Plan-Do-Study-Act (PDSA) Cycle
Authors: Montague Norma R. , Brewer Peter C. , Reid Lauren C. , Kohlmeyer James M. 
Published: May 2024

ABSTRACT
An obstacle accounting educators face in facilitating learning effectiveness is the perennial student studying strategy—the dreaded “cramming cycle.” We present a novel approach to help resolve this issue, based on W. Edwards Deming’s Plan-Do-Study-Act (PDSA) cycle. This learning strategy is intended to reduce the need for cramming by providing a systematic approach to engage students in active learning, reflection, and continuous improvement processes. Following our implementation of this approach in undergraduate and graduate managerial accounting courses, our observations indicate the PDSA method increases student preparation for class, enhances participation during class, and boosts retention of material. We provide implementation guidelines that are easily adaptable to on-ground, online, and hybrid course delivery modes.