bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adaptaciones curriculares para la inclusión de estudiantes con discapacidad a la carrera de Pedagogía de la Actividad Física y Deporte
Authors: Toledo Cueva Mauro , Velarde Bermeo Carlos Estuardo , Loaiza Dávila Lenin Esteban , Giceya de la Caridad Maqueira Caraballo 
Published: May 2024

Introducción. La presencia de estudiantes con discapacidad en la Educación Superior constituye uno de los principales retos de los sistemas educativos actuales, siendo necesario el establecimiento de manifiestos pertinentes que establezcan políticas y estatutos de carácter inclusivo, sin excepción de carrera alguna como es el caso de la Pedagogía de la Actividad Física y Deporte. Objetivo. Indagar las adaptaciones curriculares que promueven la inclusión de estudiantes con diferentes discapacidades en la Carrera de Pedagogía de la Actividad Física y Deporte en el contexto ecuatoriano. Metodología. Diseño de investigación: enfoque mixto de investigación, en su planteamiento cuantitativo diseño no experimental, descriptivo, de campo y de corte trasversal. En su planteamiento cualitativo se planteó un diseño fenomenológico. Población y muestra: 39 docentes de la Carrera de Pedagogía de la Actividad Física y Deporte de 18 universidades del Ecuador. Técnicas e instrumentos: Se aplicó la técnica cuantitativa de la encuesta y como instrumento un cuestionario auto administrado sobre los tipos de discapacidad y las adaptaciones curriculares que aplican los docentes en la carrera de Pedagogía de la Actividad Física y Deporte. En el desarrollo del planteamiento cualitativo se aplicó entrevistas en profundidad a un estudiante representante de cada tipo de discapacidad, abordando los siguientes tópicos a través de una pregunta abierta. Tratamiento estadístico de los datos: Paquete estadístico SPSS versión 25, desarrollando un análisis de valores medios y desviaciones estándares para las variables de origen cuantitativo y un análisis de frecuencias y porcentajes para las variables de origen cualitativo. Resultados. Se determinaron las adaptaciones curriculares aplicadas por los docentes en relación con la discapacidad física, visual e intelectual. Categorías generales, axiales y ejecutivas que determinaron una teoría emergente en base a las percepciones y experiencias comunicadas por parte de los sujetos intervenciones en el proceso de investigación. Conclusión. Se han realizado avances significativos en adaptaciones curriculares para estudiantes con discapacidades en la carrera de Pedagogía de la Actividad Física y Deporte, existen brechas notables en términos de inclusión efectiva y sensibilización de la comunidad educativa. Área de estudio general: Educación. Área de estudio específica: Educación Física.
Exploring the diversity of cancer-associated fibroblasts: insights into mechanisms of drug resistance
Authors: Kazakova Anastasia N. , Lukina Maria M. , Anufrieva Ksenia S. , Bekbaeva Irina V. , Ivanova Olga M. , Shnaider Polina V. , Slonov Andrey , Arapidi Georgij P. , Shender Victoria O. 
Published: May 2024

Introduction: Among the various stromal cell types within the tumor microenvironment, cancer-associated fibroblasts (CAFs) emerge as the predominant constituent, exhibiting a diverse array of oncogenic functions not intrinsic to normal fibroblasts. Their involvement spans across all stages of tumorigenesis, encompassing initiation, progression, and metastasis. Current understanding posits the coexistence of distinct subpopulations of CAFs within the tumor microenvironment across a spectrum of solid tumors, showcasing both pro- and antitumor activities. Recent advancements in single-cell transcriptomics have revolutionized our ability to meticulously dissect the heterogeneity inherent to CAF populations. Furthermore, accumulating evidence underscores the pivotal role of CAFs in conferring therapeutic resistance to tumors against various drug modalities. Consequently, efforts are underway to develop pharmacological agents specifically targeting CAFs.Methods: This review embarks on a comprehensive analysis, consolidating data from 36 independent single-cell RNA sequencing investigations spanning 17 distinct human malignant tumor types.Results: Our exploration centers on elucidating CAF population markers, discerning their prognostic relevance, delineating their functional contributions, and elucidating the underlying mechanisms orchestrating chemoresistance.Discussion: Finally, we deliberate on the therapeutic potential of harnessing CAFs as promising targets for intervention strategies in clinical oncology.
Assessment of bidirectional relationships between hypothyroidism and endometrial cancer: a two-sample Mendelian randomization study
Authors: Wang Bolin , Luo Yuxi , Liu Tianxin , Xu Shengnan , Pei Jinli , Liu Jie , Yu Jinming 
Published: May 2024

ObjectiveHypothyroidism, characterized by reduced thyroid hormone levels, and endometrial cancer, a prevalent gynecological malignancy, have been suggested to have a potential association in previous observational studies. However, the causal relationship between them remains uncertain. This study aimed to investigate the causal relationship between hypothyroidism and endometrial cancer using a bilateral Mendelian randomization approach.MethodsA bidirectional two-sample Mendelian randomization study was conducted using summary statistics from genome-wide association studies to identify genetic variants associated with hypothyroidism and endometrial cancer. The inverse variance weighting method was used as the main analysis, and sensitivity analyses were conducted to validate the MR results.ResultsThe results of our analysis did not support a causal effect of hypothyroidism (OR: 0.93, p=0.08) or autoimmune hypothyroidism (OR: 0.98, p=0.39) on endometrial cancer risk. In the reverse MR analysis, we did not find a significant causal effect of endometrial cancer on hypothyroidism (OR: 0.96, p=0.75) or autoimmune hypothyroidism (OR: 0.92, p=0.50). Based on subgroup analysis by pathological subtypes of endometrial cancer, the above findings were further substantiated (all p-value &gt;0.05).ConclusionsOur Mendelian randomization analysis suggests a lack of causal association between hypothyroidism and endometrial cancer. To gain a deeper understanding of this association, it is essential to conduct large-scale randomized controlled trials in the future to validate our findings.
Diagnostic value of FNAC combined with BRAFV600E mutation detection in Hashimoto’s thyroiditis complicated with papillary thyroid carcinoma
Authors: Fu Jingyao , Yin Xiangdang , Wang Xiaochun , Xiao Siqi , Wu Xianji , Duan Chengcheng , Yu Wenxi , Zhang Guang 
Published: May 2024

BackgroundThis study aimed to analyze the effect of preoperative fine needle aspiration cytology (FNAC) combined with BRAFV600E mutation detection as compared to that of fine needle aspiration cytology alone on the diagnostic performance of papillary thyroid carcinoma (PTC) combined with Hashimoto’s thyroiditis (HT).MethodPatients with thyroid nodules in Hashimoto’s thyroiditis, who underwent fine-needle aspiration cytology examination and BRAFV600E mutation detection in the puncture eluate at the outpatient clinic, were selected. Finally, 122 patients received surgical treatment and were included in the study. We used postoperative pathological results as the gold standard. Accordingly, we compared the sensitivity, specificity and accuracy of preoperative FNAC alone and FNAC combined with BRAFV600E mutation detection in for the diagnosis of PTC combined with HT.ResultsFor PTC patients with HT, the sensitivity of FNAC diagnosis was 93.69%, the specificity was 90.90% and the accuracy was 93.44%. However, the sensitivity, specificity and accuracy of FNAC combined with BRAFV600E mutation detection were 97.30%, 90.90% and 96.72%, respectively. Therefore, combined detection can improve the sensitivity and accuracy of diagnosis (p&lt;0.05).ConclusionFNAC combined with eluent BRAFV600E mutation detection can improve the sensitivity and accuracy of diagnosis of PTC in the background of HT.
Nanotechnology boosts the production of clean energy via nanoparticle addition in anaerobic digestion
Authors: Castro J. Cunha de , Resende E. , Taveira Igor , Enrich-Prast A. , Abreu F. 
Published: May 2024

Biogas production via anaerobic digestion is an established and robust technology that produces energy and recycles nutrients. Several biotechnological attempts have been applied to this process to increase biogas production, including adding nanoparticles, but several discrepancies have been reported. To elucidate the contradictory results, we performed a literature review followed by a meta-analysis to evaluate the effect of adding natural nanoparticles to biogas sludge. Our results showed that adding nanoparticles can increase biogas production by up to two orders of magnitude. Considering that, we attribute these results to variability in the nanoparticles applied, leading to less reliable, consistent, and even contradictory results. We observed that the magnetite nanoparticles are the most tested ones with the most promising positive effects. In addition, we observed that concentrations of nanoparticles higher than 100 mg/L can have adverse effects, with an overall decrease in biogas production. The findings in this study highlight the need for a proper characterization of the nanomaterials type and concentration applied to the process to understand the interactions and effects on the microbial communities and dynamics that lead to an overall increase or decrease in biogas yield.
Counting dense object of multiple types based on feature enhancement
Authors: Fu Qiyan , Min Weidong , Sheng Weixiang , Peng Chunjiang 
Published: May 2024

IntroductionAccurately counting the number of dense objects in an image, such as pedestrians or vehicles, is a challenging and practical task. The existing density map regression methods based on CNN are mainly used to count a class of dense objects in a single scene. However, in complex traffic scenes, objects such as vehicles and pedestrians usually exist at the same time, and multiple classes of dense objects need to be counted simultaneously.MethodsTo solve the above issues, we propose a new multiple types of dense object counting method based on feature enhancement, which can enhance the features of dense counting objects in complex traffic scenes to realize the classification and regression counting of dense vehicles and people. The counting model consists of the regression subnet and the classification subnet. The regression subnet is primarily used to generate two-channel predicted density maps, mainly including the initial feature layer and the feature enhancement layer, in which the feature enhancement layer can enhance the classification features and regression counting features of dense objects in complex traffic scenes. The classification subnet mainly supervises classifying dense vehicles and people into two feature channels to assist the regression counting task of the regression subnets.ResultsOur method is compared on VisDrone+ datasets, ApolloScape+ datasets, and UAVDT+ datasets. The experimental results show that the method counts two kinds of dense objects simultaneously and outputs a high-quality two-channel predicted density map. The counting performance is better than the state-of-the-art counting network in dense people and vehicle counting.DiscussionIn future work, we will further improve the feature extraction ability of the model in complex traffic scenes to classify and count a variety of dense objects such as cars, pedestrians, and non-motor vehicles.
Integrative analysis of transcriptomics and metabolomics reveals the protective effect and mechanism of salidroside on testicular ischemia-reperfusion injury
Authors: Jiang Ya Ping , Liu Bao Gui , Dang Yi , Liu Lin Jie , Pang Yang , Bai Xiao Dong , Sun Feng , Kang Tian Hong , Zhao Zheng Hang 
Published: May 2024

Testicular torsion is a critical urologic condition for which testicular detorsion surgery is considered irreplaceable as well as the golden method of reversal. However, the surgical treatment is equivalent to a blood reperfusion process, and no specific drugs are available to treat blood reperfusion injuries. Salidroside (SAL) is one of the main effective substances in rhodiola, which has been shown to have antioxidant and antiapoptosis activities. This study was designed to determine whether SAL exerted a protective effect on testicular ischemia-reperfusion (I/R) injury. In this study, the I/R injury model of the testes and reoxygenation (OGD/R) model were used for verification, and SAL was administered at doses of 100 mg/kg and 0.05 mmol/L, respectively. After the experiments, the testicular tissue and TM4 Sertoli cells were collected for histopathologic and biochemical analyses. The results revealed that SAL improves the structure of testicular tissue and regulates the oxidation–antioxidation system. To further understand the molecular mechanisms of SAL in treating testicular I/R injuries, transcriptomics and metabonomics analyses were integrated. The results show that the Nfr2/HO-1/GPX4/ferroptosis signaling pathway is enriched significantly, indicating that it may be the main regulatory pathway for SAL in the treatment of testicular I/R injuries. Thereafter, transfection with Nrf2 plasmid–liposome was used to reverse verify that the Nfr2/HO-1/GPX4/ferroptosis signaling pathway was the main pathway for SAL anti-testicular I/R injury treatment. Thus, it is suggested that SAL can protect against testicular I/R injuries by regulating the Nfr2/HO-1/GPX4 signaling pathway to inhibit ferroptosis and that SAL may be a potential drug for the treatment of testicular I/R injuries.
Nitrogen migration and transformation characteristics of the soil in karst areas under the combined application of oxalic acid and urea inhibitors
Authors: Jiafeng Wang , Qiuliang Cai 
Published: May 2024

ObjectiveWe investigated the horizontal migration and transformation of nitrogen in soil with oxalic acid and inhibitors (e.g., nitrification inhibitors, DMPP, urease inhibitors, and NBPT) under different soil water contents to provide a basis for the efficient utilization of nitrogen fertilizer in agricultural production in karst areas.MethodsFour nitrogen fertilizers (e.g., ammonium bicarbonate, ammonium sulfate, ammonium chloride, and urea) were applied separately and combined with oxalic acid, DMPP, and NBPT. The ammonium and nitrate nitrogen contents in the different soil layers were measured. The soil columns were cultured through an indoor soil column simulation at water content levels of 30%, 40%, and flooded (50%) for 30 days.ResultsAmmonium bicarbonate with inhibitors increased soil NH4+-N content by 15.42–21.12%. Ammonium sulfate with oxalic acid or NBPT increased soil NH4+-N content by 27.56–52.25% at 30% and 40% moisture content treatments, compared to ammonium sulfate alone. Urea with DMPP application significantly increased soil NH4+-N content by 11.93–14.87% at 40% water content and flooded conditions. In all treatments, the NH4+-N content in the soil treated with 30% water content of ammonium chloride with oxalic acid was the highest. The NH4+-N content showed a decreasing trend with an increase in the water content. The NO3−-N content in soil treated with ammonium bicarbonate and DMPP was higher than that treated with other nitrogen fertilizers at 30% moisture. The NO3−-N content decreased with increased water content. Under all treatments, ammonium chloride with oxalic acid had the highest percentage of soil NH4+-N and soil soluble inorganic nitrogen at 30% water content, with 55.29% and 55.97%, respectively.ConclusionAmong the nitrogen fertilizer treatments, the soil NH4+-N content increased in ammonium bicarbonate with DMPP or NBPT, ammonium sulfate with oxalic acid or NBPT, and urea with DMPP. The four nitrogen fertilizers with DMPP increased the soil NO3−-N content. Nitrogen fertilizer combined with oxalic acid and inhibitors could effectively improve the effective use of nitrogen fertilizer.
Multimorbidity patterns and trajectories in young and middle-aged adults: a large-scale population-based cohort study
Authors: Ioakeim-Skoufa Ignatios , González-Rubio Francisca , Aza-Pascual-Salcedo Mercedes , Laguna-Berna Clara , Poblador-Plou Beatriz , Vicente-Romero Jorge , Coelho Helena , Santos-Mejías Alejandro , Prados-Torres Alexandra , Moreno-Juste Aida , Gimeno-Miguel Antonio 
Published: May 2024

IntroductionThe presence of multiple chronic conditions, also referred to as multimorbidity, is a common finding in adults. Epidemiologic research can help identify groups of individuals with similar clinical profiles who could benefit from similar interventions. Many cross-sectional studies have revealed the existence of different multimorbidity patterns. Most of these studies were focused on the older population. However, multimorbidity patterns begin to form at a young age and can evolve over time following distinct multimorbidity trajectories with different impact on health. In this study, we aimed to identify multimorbidity patterns and trajectories in adults 18–65 years old.MethodsWe conducted a retrospective longitudinal epidemiologic study in the EpiChron Cohort, which includes all inhabitants of Aragón (Spain) registered as users of the Spanish National Health System, linking, at the patient level, information from electronic health records from both primary and specialised care. We included all 293,923 patients 18–65 years old with multimorbidity in 2011. We used cluster analysis at baseline (2011) and in 2015 and 2019 to identify multimorbidity patterns at four and eight years of follow-up, and we then created alluvial plots to visualise multimorbidity trajectories. We performed age- and sex-adjusted logistic regression analysis to study the association of each pattern with four- and eight-year mortality.ResultsWe identified three multimorbidity patterns at baseline, named dyslipidaemia &amp; endocrine-metabolic, hypertension &amp; obesity, and unspecific. The hypertension &amp; obesity pattern, found in one out of every four patients was associated with a higher likelihood of four- and eight-year mortality (age- and sex-adjusted odds ratio 1.11 and 1.16, respectively) compared to the unspecific pattern. Baseline patterns evolved into different patterns during the follow-up.DiscussionWell-known preventable cardiovascular risk factors were key elements in most patterns, highlighting the role of hypertension and obesity as risk factors for higher mortality. Two out of every three patients had a cardiovascular profile with chronic conditions like diabetes and obesity that are linked to low-grade systemic chronic inflammation. More studies are encouraged to better characterise the relatively large portion of the population with an unspecific disease pattern and to help design and implement effective and comprehensive strategies towards healthier ageing.
Breast Cancer Awareness and Screening Perceptions of Women in Yerevan, Armenia
Authors: Tupper Haley , Ghukasyan Razmik , Bayburtyan Armine , Hovhannisyan Marine , Shekherdimian Shant 
Published: May 2024

Objectives:Breast cancer is the leading cause of female cancer mortality in Armenia. The government is considering covering breast cancer screening, but prevailing attitudes towards it are unknown. This cross-sectional study assessed Armenian women’s awareness and perceptions of breast cancer screening.Methods:We administered a validated telephone survey to women ages 35–65 registered in Yerevan’s polyclinic system between 2019–2021, assessing sociodemographic characteristics, breast cancer exposure and screening attitudes, using an adapted Champion’s Health Belief Model Scale (CHBMS). We analyzed the association, unadjusted and adjusted, between sociodemographic characteristics, screening exposure, and CHBMS scores.Results:170 women completed surveys. Most (82.9%) were aware of screening, 48.5% knew someone with breast cancer, but only 42.5% had undergone screening, predominantly without their physician’s recommendation (63.2%). Despite elevated awareness, 76.2% had never discussed screening with their provider. Barriers included cost and mistreatment concerns. Education consistently predicted prior screening and most CHBMS scores.Conclusion:Armenian women are highly exposed to breast cancer, but knowledge and prior screening primarily emanate from non-physician sources. Results highlighted the influence of education, patient-provider relationships, and healthcare costs, underscoring the importance of multi-level interventions.
Rumi and Pasteurized Kareish Cheeses Are a Source of β-Lactam-Resistant Salmonella in the Nile Delta Region of Egypt: Insights into Their Incidence, AMR Pattern, Genotypic Determinants of Virulence and β-Lactam Resistance
Authors: Elzhraa Fatma , Al-Ashmawy Maha , El-Sherbini Mohammed , El-Sebaey Ahmed M. , Mohácsi-Farkas Csilla , Kiskó Gabriella , Belák Ágnes 
Published: May 2024

The spread of superbugs in dairy products can jeopardize global public health. To date, information on the incidence rates of virulent and β-lactams-resistant (BLR) Salmonella in cheeses from rural areas of Egypt has been lacking. Biochemical, serological, antibiotic susceptibility, and multiplex PCR (M-PCR) tests were performed to identify and characterize Salmonella isolates. In this study, 44 (15.71%) Salmonella isolates of eight different serotypes were recovered from 280 samples of Rumi and pasteurized Kariesh cheeses across the Nile Delta region of Egypt. The most predominant serotypes were S. Typhimurium, S. Enteritidis, and S. Infantis. The virulence genes (invA, stn, and hilA) were identified in all isolates. However, spvC was only detected in S. Typhimurium. The highest resistance was developed against Erythromycin and Clindamycin (90.91%), followed by Ceftazidime and Cephalothin (84.09%). Meropenem and colistin were the most effective antibiotics. A high proportion (79.55%) of multi-drug resistance (MDR) isolates carried narrow spectrum (NS), extended-spectrum (ES), and AmpC-BLR genes. The blaOXA-1, blaOXA-2, blaTEM-1, blaCTX-M, blaCMY-1, and blaCMY-2 BLR genes were positive in 37.04%, 29.63%, 25.93%, 14.81%, 37.04%, and 3.70% of isolates, respectively. In conclusion, a high prevalence of virulence and BLR genes harboring Salmonella strains in Egyptian cheeses is considered a great threat to public health.