bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of exogenous histone H1 on rat adipose‐derived stem cell proliferation, migration, and osteogenic differentiation in vitro
Authors: Hsu Li‐Wen , Goto Shigeru , Nakano Toshiaki , Chen Kuang‐Den , Wang Chih‐Chi , Lai Chia‐Yun , Hou Chiung‐Hui , Chang Yen‐Chen , Cheng Yu‐Fan , Chiu King‐Wah , Chen Chien‐Chih , Chen Shu‐Hui , Chen Chao‐Long 
Published: December 2011

AbstractAdipose‐derived stem cells (ASCs) are of great interest for the development of novel cell therapies due to their ease of isolation and expansion, immunosuppressive activity, and multilineage differentiation potential. However, the mechanisms underlying the therapeutic potential of ASCs remain to be elucidated. Others and we have shown that nuclear proteins such as histone H1 and high mobility group box 1 (HMGB1) play important roles in the maturation of dendritic cells (DCs). Furthermore, we previously demonstrated translocation of histone H1 from the nucleus to the cytoplasm and activation of mitogen‐activated protein kinases (MAPKs) in DCs. In the present study, we confirmed that histone H1 does not alter the immunophenotype and immunosuppression potential of ASCs, but that histone H1 enhanced wound healing and increased interleukin (IL)‐6 expression. Moreover, histone H1 treated‐ASCs showed up‐regulation of MAPKs extracellular‐regulated kinase 1/2 (ERK1/2) and sequential NF‐κB translocation. Finally, we found that culture in differentiation media supplemented with histone H1 enhanced ASC osteogenesis. In contrast, inhibition of histone H1 by small interfering RNA (siRNA) reduced osteogenic differentiation markers including ALP. These results suggest that histone H1 may be useful for induction of mesenchymal stem cells in tissue engineering and future potential ASC therapies. J. Cell. Physiol. 227: 3417–3425, 2012. © 2011 Wiley Periodicals, Inc.
Scriptaid effects on breast cancer cell lines
Authors: Giacinti L. , Giacinti C. , Gabellini C. , Rizzuto E. , Lopez M. , Giordano A. 
Published: December 2011

AbstractIn breast cancer tumor expression of estrogen receptors (ERs) is important as a marker of prognosis and mostly as a predictor of response to endocrine therapy. In fact, the loss of α‐ER expression leads to unresponsiveness to anti‐hormone treatment. In a significant fraction of breast cancers, this loss of expression is a result of epigenetic mechanisms, such as DNA methylation and histone deacetylation, within the α‐ER promoter. Previous studies have shown that pharmacologic inhibition of these mechanisms using the DNA methyltransferase inhibitor, 5‐aza‐2‐deoxycytidine (AZA), and the histone deacetylase (HDAC) inhibitor, Trichostatin A (TSA), results in expression of functional α‐ER mRNA and protein. Moreover, the activity of a novel HDAC inhibitor, Scriptaid, has been shown to induce inhibition of tumor growth in breast cancer and to cause re‐expression of functional α‐ER in α‐ER negative breast cancer cells. We sought to better characterize the effects of Scriptaid on cell growth, apoptosis, and α‐ER expression in α‐ER‐positive (MCF‐7), α‐ER‐negative (MDA‐MB‐231), and α‐ER‐negative/Her‐2 over‐expressing (SKBr‐3) human breast cancer cell lines. In all of these cell lines Scriptaid treatment resulted in significant growth inhibition and apoptosis, and RT‐PCR confirmed an increase of α‐ER mRNA transcript in MDA‐MB‐231 after 48 h of Scriptaid treatment. Furthermore, following treatment with Scriptaid, the formerly unresponsive MDA‐MB‐231 and SKBr‐3 breast cancer cells became responsive to tamoxifen. These results show that the HDAC inhibitor Scriptaid is able to sensitize tamoxifen hormone‐resistant breast cancer cells, and that Scriptaid or related HDAC inhibitors are candidates for further study in breast cancer. J. Cell. Physiol. 227: 3426–3433, 2012. © 2011 Wiley Periodicals, Inc.
The filler content of the dental composite resins and their influence on different properties
Authors: Rastelli Alessandra N.S. , Jacomassi Denis P. , Faloni Ana Paula S. , Queiroz Thallita P. , Rojas Seila S. , Bernardi Maria INÊs B. , Bagnato Vanderlei S. , Hernandes AntÔnio C. 
Published: December 2011

AbstractThe purpose of this study was to compare the inorganic content and morphology of one nanofilled and one nanohybrid composite with one universal microhybrid composite. The Vickers hardness, degree of conversion and scanning electron microscope of the materials light‐cured using LED unit were also investigated. One nanofilled (Filtek™ Supreme XT), one nanohybrid (TPH®3) and one universal microhybrid (Filtek™ Z‐250) composite resins at color A2 were used in this study. The samples were made in a metallic mould (4 mm in diameter and 2 mm in thickness). Their filler weight content was measured by thermogravimetric analysis (TG). The morphology of the filler particles was determined using scanning electron microscope equipped with a field emission gun (SEM‐FEG). Vickers hardness and degree of conversion using FT‐IR spectroscopy were measured. Filtek™ Z‐250 (microhybrid) composite resin shows higher degree of conversion and hardness than those of Filtek™ Supreme XT (nanofilled) and TPH®3 (nanohybrid) composites, respectively. The TPH3® (nanohybrid) composite exhibits by far the lowest mechanical property. Nanofilled composite resins show mechanical properties at least as good as those of universal hybrids and could thus be used for the same clinical indications as well as for anterior restorations due to their high aesthetic properties. Microsc. Res. Tech. 75:758–765, 2012. © 2011 Wiley Periodicals, Inc
Comparing different preparation methods to study human fibrin fibers and platelets using TEM
Authors: Buys Antoinette V. , Pretorius Etheresia 
Published: December 2011

AbstractFor the study of cellular ultrastructure, the sample needs to be stabilized by fixation, with the ultimate aim to preserve the native tissue organization and to protect the tissue against later stages of preparation. Chemical and freezing fixation are most used, and chemical fixation employs agents that permeate tissues and cells by diffusion and covalently bind with their major biochemical constituents to fix them. Most widely used chemical fixatives are aldehydes, e.g., formaldehyde and glutaraldehyde, which are noncoagulating, crosslinking agents. Cryofixation methods for ultrastructural studies are also popular, and high‐pressure freezing immobilizes all cell constituents and arrests biological activity by removing the thermal energy from the system. In the current research, we used platelet‐rich plasma (PRP) to study expansive fibrin fibers and platelet ultrastructure to compare the two fixation techniques. We also used thrombin and calcium chloride as a clotting agent to determine the technique most suitable for the formation of extensive fibrin networks. Chemically fixated fibrin fibers were more compact and condensed and also showed a banding pattern on longitudinal sections. High‐pressure frozen samples were more dispersed while platelets fixated showed better preserved cellular membranes and organelle structure. PRP coagulated by addition of CaCl2 showed blood platelets that are noticeably more activated compared with PRP; however, with thrombin, a sharp ultrastructure was seen. We conclude that PRP mixed with thrombin, and freeze substituted, is the most suitable method for the study of extensive fibrin fibers as well as platelets. Microsc. Res. Tech. 75:801–806, 2012. © 2011 Wiley Periodicals, Inc.
Morphological analysis of colon goblet cells and submucosa in type I diabetic rats submitted to physical training
Authors: Remedio Rafael Neodini , Castellar Alexandre , Barbosa Rodrigo Avelaira , Gomes Ricardo JosÉ , Caetano FlÁVio Henrique 
Published: December 2011

AbstractColon layers, especially the submucosa, as well as the secretion of goblet cells are extremely important for the functioning and transit of substances in this organ. However, the damages arising from type I diabetes and the effects of physical training, which plays crucial role in the treatment of this disease, are not yet known in these regions. To analyze the changes in colon submucosa and goblet cells of diabetic rats, as well as the effects of physical training, Wistar rats were divided into four groups: sedentary control, trained control, sedentary diabetic (SD), and trained diabetic (TD). The training protocol consisted of swimming for 60 min a day, 5 days per week, during 8 weeks. Colon samples were collected, processed, and evaluated by histochemical and ultrastructural techniques. Goblet and submucosa cells did not show alterations in shape, size, protein and carbohydrate content, in all treatment groups. Decreased amount of collagen fibers, however, was observed in the submucosa and lamina propria of SD rats, but this alteration was recovered in TDs. The ultrastructural analysis, in turn, revealed greater quantity of Golgi apparatus cisterns in SDs, distinctly than TDs, which showed improvement in this diabetic condition. Thus, physical training was responsible for the recovery of some important diabetic alterations, possibly improving the motility of substances in the large intestine. Nevertheless, it cannot be considered alone in the treatment of this disease, requiring the combined practice of other methods. Microsc. Res. Tech. 75:821–828, 2012. © 2011 Wiley Periodicals, Inc.
Toward a more realistic prediction of peri‐prosthetic micromotions
Authors: van der Ploeg Bas , Tarala Maria , Homminga Jasper , Janssen Dennis , Buma Pieter , Verdonschot Nico 
Published: December 2011

AbstractThe finite element (FE) method has become a common tool to evaluate peri‐prosthetic micromotions in cementless total hip arthroplasty. Often, only the peak joint load and a selected number of muscle loads are applied to determine micromotions. Furthermore, the applied external constraints are simplified (diaphyseal fixation), resulting in a non‐physiological situation. In this study, a scaled musculoskeletal model was used to extract a full set of muscle and hip joint loads occurring during a walking cycle. These loads were applied incrementally to an FE model to analyze micromotions. The relation between micromotions and external loads was investigated, and how micromotions during a full loading cycle compared to those calculated when applying a peak load only. Finally, the effect of external constraints was analyzed (full model vs. diaphyseal fixation and reduced number of muscle loads). Relatively large micromotions were found during the swing phase when the hip joint forces were relatively low. Maximal micromotions, however, did concur with the peak hip joint force. Applying only a peak joint force resulted in peak micromotions similar to those found when full walking cycle loads were applied. The magnitude and direction of the micromotions depended on the applied muscle loads, but not on external constraints. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:1147–1154, 2012
Flavokawain B, a kava chalcone, induces apoptosis in synovial sarcoma cell lines
Authors: Sakai Toshinori , Eskander Ramez N. , Guo Yi , Kim Kap Jung , Mefford Jason , Hopkins Justin , Bhatia Nitin N. , Zi Xiaolin , Hoang Bang H. 
Published: December 2011

AbstractSynovial sarcomas (SS) are soft tissue sarcomas with poor prognosis, displaying a lack of response to conventional cytotoxic chemotherapy. Although SS cell lines have moderate chemosensitivity to isofamide and doxorubicin therapy, the clinical prognosis is still poor. In this article, we showed that flavokawain B (FKB), a novel chalcone from kava extract, potently inhibits the growth of SS cell lines SYO‐I and HS‐SY‐II through induction of apoptosis. Treatment with FKB increased caspase 8, 9, and 3/7 activity compared to vehicle‐treated controls, indicating that both extrinsic and intrinsic apoptotic pathways were activated. Furthermore, FKB treatment of both cell lines resulted in increased mRNA and protein expression of death receptor‐5 and the mitochondrial pro‐apoptotic proteins Bim and Puma, while down‐regulating the expression of an inhibitor of apoptosis, survivin in a dose‐dependent manner. Our results suggest the natural compound FKB has a pro‐apoptotic effect on SS cell lines. FKB may be a new chemotherapeutic strategy for patients with SS and deserves further investigation as a potential agent in the treatment of this malignancy. © 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:1045–1050, 2012
Bayesian hierarchical model assessment of nest site and landscape effects on nest survival of aplomado falcons
Authors: Brown Jessi L. , Collopy Michael W. 
Published: December 2011

AbstractWe investigated potential effects of nest site and landscape scale factors, including anthropogenic disturbance and habitat patchiness, on the nesting success of a reintroduced population of northern aplomado falcons (Falco femoralis septentrionalis) in southern Texas. We monitored 62 nesting attempts during 2002–2004 in the Lower Rio Grande Valley. We developed hierarchical models describing daily nest survival rates (DSR) and compared the models using a Bayesian approach in R and WinBUGS. We considered possible effects of nest age, temporal trends, nest site variables, landscape structure, territory (a random effect), and 3 measures of anthropogenic disturbance: distance to paved road, proximity to power pole, and nocturnal light intensity. Whether evaluated by Deviance Information Criterion (DIC) scores or the models' overall posterior probabilities as estimated with a reversible jump Markov Chain Monte Carlo algorithm, none of our landscape or disturbance measures affected DSR. Rather, variation in DSR was best described by nest height, overhead cover, and nest source (artificial or natural). These nest site level factors may be manipulated by managers through provision of artificial nests. We recommend that artificial nests continue to be provided, as such nests are highly successful when located on moderately tall substrates, and they permit researchers to access nest contents for population monitoring. © 2011 The Wildlife Society.
Polyelectrolyte Complex Materials Consisting of Antibacterial and Cell‐Supporting Layers
Authors: Mat Amin Khairul Anuar , Gilmore Kerry J. , Matic Jake , Poon Stephen , Walker Mark J. , Wilson Mark R. , in het Panhuis Marc 
Published: December 2011

AbstractThe characterization of a polyelectrolyte complex material comprised of two biopolymers, a chitosan upper layer and a gellan gum under layer, is reported. It is shown that the upper layer of chitosan with incorporated levofloxacin displays an antibacterial activity, while the under layer of a gellan gum/TiO2 composite supports the growth of fibroblastic cells.magnified image
Controlled Cell Proliferation on an Electrochemically Engineered Collagen Scaffold
Authors: Gendron Robert , Kumar M. Ramesh , Paradis Helene , Martin Darryl , Ho Nhu , Gardiner Danielle , Merschrod S. Erika F. , Poduska Kristin M. 
Published: December 2011

AbstractTherapies for corneal disease and injury often rely on artificial implants, but integrating cells into synthetic corneal materials remains a significant challenge. The electrochemically formed collagen‐based matrix presented here is non‐toxic to cells and controls the proliferation in the corneal fibroblasts seeded onto it. Histology and biomolecular studies show a behavior similar to corneal stromal cells in a native corneal environment. Not only is this result an important first step toward developing a more realistic, multi‐component artificial cornea, but it also opens possibilities for using this matrix to control and contain the growth of cells in engineered tissues.magnified image
Internal erosion of chemically reinforced granular materials: a mathematical modeling approach
Authors: Khalil Tony , Saiyouri Nadia , Muresan Bogdan , Hicher Pierre‐Yves 
Published: December 2011

SUMMARYInternal erosion (IE) affects the stability of natural and reinforced materials by causing instability within their granular structure. The dislodgement and transport of eroded particles affect both the particulate concentration of eroding fluid and the pore network of eroded material. In this study, we examined these modifications using a transport model with a finite element code. First, IE tests on chemically reinforced sand columns were performed to obtain information about eroded material loss of mass, particulate concentration of effluent, porosity and permeability modifications, and existing IE stages. Second, based on experimental results, a mathematical one‐dimensional model has been formulated to monitor the evolution and spatial distribution of erodible solids, fluidized particles, porosity, permeability, and seepage stresses. The model consists of a set of coupled nonlinear differential equations solved in sequence. It provides valuable information about the extent and the dynamics of structural changes, which can be used to estimate an IE time for the hydraulic work to reach failure. Copyright © 2011 John Wiley & Sons, Ltd.
Japanese Older Adults' Perspectives on Resuming Daily Life During Hospitalization and After Returning Home
Authors: Bontje Peter , Asaba Eric , Tamura Yumi , Josephsson Staffan 
Published: December 2011

AbstractThroughout Japan, occupational therapy for older adults is available in an increasing array of institutional and community settings. However, there is a need for more knowledge of “how” older adults resume their daily lives particularly in the community. The aim of this qualitative research was to identify and describe how some older adults in Japan describe their experiences of resuming daily life during hospitalization and after returning home. Nine older adults with various physical impairments were interviewed. The open interviews were conducted 10 months to 5 years from the onset of their physical impairment. Data analysis on the basis of a constant comparative methodology resulted in three themes that characterize their experiences of resuming daily life: “doing the right thing”, “reconciling to dependence” and “becoming invigorated”.These themes may inform occupational therapists to take into consideration older clients' perspectives when assisting them to recapture the quality of their daily life according to their preferences. Adaptive change is promoted by assisting older clients to generate the energy they need by judiciously varying approaches appropriate to their goals and by helping older clients to make decisions that are “right” for them, which may fluctuate during processes of resuming daily life. Limitations of this study are found in the small sample size, and additional research may help to clarify how moral aspects, vigour (physical and mental energy/force) and other dimensions shape processes of resuming daily life as well as the influences of time. Copyright © 2011 John Wiley & Sons, Ltd.
Electro‐optical properties of polymer dispersed liquid crystal prepared by successively controlled living radical polymerization
Authors: Qin Ailin , Sun Zhen , Zhang Yuliang , Wang Yinghan 
Published: December 2011

AbstractPolymer dispersed liquid crystal (PDLC) films were obtained by successive controlled living radical polymerizations: starting polystyrene (M1) was obtained by reversible addition‐fragmentation polymerization (RAFT), M1 was converted to P‐chloromethylated polystyrene (M2) which was grafted with polystyrene branches by atom transfer radical polymerization (ATRP) to yield RAFT‐initiating graft polymer containing trithiocarbonate moieties in the backbone (M3, RAFT‐active grafted polystyrene), and then PDLC films were prepared by photo‐induced RAFT copolymerization of methyl acrylate with M3 in the presence of a nematic liquid crystal. The electro‐optical properties of the films were investigated for the purpose to apply them to optical devices. Experimental results showed that preferable properties could be acquired by controlling the amount of M3 and the liquid crystal E7 in the polymer matrix of PDLC films. POLYM. COMPOS., 2012. © 2011 Society of Plastics Engineers
Electrochemical investigations on the corrosion protection effect of poly(vinyl carbazole)‐silica hybrid sol–gel materials
Authors: Kuo Tai‐Hung , Weng Chang‐Jian , Chen Chi‐Lun , Chen Ya‐Lun , Chang Chi‐Hao , Yeh Jui‐Ming 
Published: December 2011

AbstractA series of sol–gel derived organic–inorganic hybrid coatings consisting of organic poly (vinyl carbazole) (PVK) and inorganic silica (SiO2), with 3‐(trimethoxysilyl)propyl methacrylate (MSMA) as coupling agent, were successfully synthesized. First of all, vinyl carbazole (VCz) monomers are copolymerized with MSMA by performing free‐radical polymerization reactions with AIBN as initiator. Subsequently, as‐prepared copolymer (i.e., sol–gel precursor) was further reacted with various feeding content of tetraethyl orthosilicate (TEOS) through organic acid (CSA)‐catalyzed sol–gel reaction to form a series of PVK‐silica hybrid (PSH) sol–gel materials. The as‐synthesized hybrid materials were subsequently characterized by Fourier‐Transformation infrared (FTIR) spectroscopy and solid‐state 29Si NMR. It should be noted that the PVK‐SiO2 hybrid (PSH) coating on cold‐rolled steel (CRS) electrode with low silica loading (e.g., 10 phr) was found to be superior in anticorrosion property over those of neat PVK based on a series of electrochemical measurements such as corrosion potential, polarization resistance, corrosion current, and electrochemical impedance spectroscopy in 3.5 wt% NaCl electrolyte. The better anticorrosion performance of PSH coatings as compared to that of neat polymer may probably be attributed to the stronger adhesion strength of PSH coatings on CRS electrode, which was further evidenced by Scotch tape test evaluation. Increase of adhesion strength of PSH coatings on CRS electrode may be associated with the formation of Fe–O–Si covalent bonds at the interface of PSH coating and CRS electrode based on the FTIR–RAS (reflection absorption spectroscopy) studies. POLYM. COMPOS., 2012. © 2011 Society of Plastics Engineers
The role of nanofillers on the degradation behavior of polylactic acid
Authors: Kontou Evagelia , Georgiopoulos Panayiotis , Niaounakis Michael 
Published: December 2011

AbstractThis study examines the effect of type and content of various nanofillers on the aging behavior of polylactic acid (PLA)/nanocomposites compared with that of pristine PLA, under specific environmental conditions, namely 80% relative humidity, 40°C temperature, and exposure time up to 6 months. Two different types of nanosized fillers (silica and montmorillonite, MMT) at three different weight fractions as well their mixtures were used for this purpose. The role of the various nanofillers on the aging of pristine PLA and its nanocomposites was investigated in terms of several experimental techniques including Scanning Electron Microscopy (SEM), Differential Scanning Calorimetry (DSC), Dynamic Mechanical Analysis (DMA), and tensile testing. All studied samples, except those of PLA/MMT/Si nanocomposites, exhibited the same trend in properties during aging. In particular, no significant changes are noted after 1 month of aging. The materials after 3 months of aging experienced a dramatic lowering of the stress–strain curve, whereas at 6 months a reverse effect was observed. Comparing the effect of nanofiller types on the degradation, one could conclude that PLA/MMT nanocomposites exhibit a more homogeneous degradation effect, having a higher impact on yield stress in comparison with silica. In contrast, silica has a greater effect in Young's modulus and strain at break, compared with MMT. The peculiar behavior of the PLA/MMT/Si nanocomposites during aging is explained by the hindering action of the mixture of the two nanofillers on the molecular reordering of the PLA chains in the amorphous region. POLYM. COMPOS., 2012. © 2011 Society of Plastics Engineers