bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selective Two‐Step Labeling of Proteins with an Off/On Fluorescent Probe
Authors: Hirabayashi Kazuhisa , Hanaoka Kenjiro , Shimonishi Manabu , Terai Takuya , Komatsu Toru , Ueno Tasuku , Nagano Tetsuo 
Published: November 2011

AbstractWe present a novel design strategy for off/on fluorescent probes suitable for selective two‐step labeling of proteins. To validate this strategy, we designed and synthesized an off/on fluorescent probe, 1–Ni2+, which targets a cysteine‐modified hexahistidine (His) tag. The probe consists of dichlorofluorescein conjugated with nitrilotriacetic acid (NTA)‐Ni2+ as the His‐tag recognition site and a 2,4‐dinitrophenyl ether moiety, which quenches the probe’s fluorescence by photoinduced electron transfer (PeT) from the excited fluorophore to the 2,4‐dinitrophenyl ether (donor‐excited PeT; d‐PeT) and also has reactivity with cysteine. His‐tag recognition by the NTA–Ni2+ moiety is followed by removal of the 2,4‐dinitrophenyl ether quencher by proximity‐enhanced reaction with the cysteine residue of the modified tag; this results in a marked fluorescence increase. Addition of His‐tag peptide bearing a cysteine residue to aqueous probe solution resulted in about 20‐fold fluorescence increment within 10 min, which is the largest fluorescence enhancement so far obtained with a visible light‐excitable fluorescent probe for a His‐based peptide tag. Further, we successfully visualized CysHis6‐peptide tethered to microbeads without any washing step. The probe also showed a large fluorescence increment in the presence of His6Cys‐tagged enhanced blue fluorescent protein (EBFP), but not His6‐tagged EBFP. We consider this system is superior to large fluorescence tags (e.g., green fluorescent protein: 27 kDa), which can perturb protein folding, trafficking and function, and also to existing small tags, which generally show little fluorescence increase upon target recognition and therefore require a washout step. This strategy should also be applicable to other tags.
Virtuous Leaders: Assessing Character Strengths in the Workplace
Authors: Thun Balliett , Kevin Kelloway E. 
Published: November 2011

AbstractThree studies were conducted to build, test, and compare a model of character‐based leadership. The first qualitative study used the critical incident technique (Flanagan, 1949) and Peterson and Seligman's (2004) comprehensive theoretical framework of character strengths to develop and construct a scale. The second study tested the scale's psychometric properties. Exploratory factor analysis indicated that character‐based leadership is a three‐dimensional construct and demonstrates adequate content validity. The third study tested the new scale against organizational and personal outcome measures. The resulting structural model provided a strong fit. Relationships emerged between leader wisdom and employee affective commitment, leader humanity and employee wellbeing, employee organizational citizenship behaviours (OCBs), employee cognitive trust, and employee affective trust, and between leader temperance and employee trust. Copyright © 2011 ASAC. Published by John Wiley & Sons, Ltd.
CD40 engagement of CD4+CD40+ T cells in a neo‐self antigen disease model ablates CTLA‐4 expression and indirectly impacts tolerance
Authors: Carter Jessica , Vaitaitis Gisela M. , Waid Dan M. , Wagner David H. 
Published: November 2011

AbstractBiomarkers defining pathogenic effector T (Teff) cells slowly have been forthcoming and towards this we identified CD4+ T cells that express CD40 (CD4+CD40+) as pathogenic in the NOD type 1 diabetes (T1D) model. CD4+CD40+ T cells rapidly and efficiently transfer T1D to NOD.scid recipients. To study the origin of CD4+CD40+ T cells and disease pathogenesis, we employed a dual transgenic model expressing OVA323–339 peptide as a neo‐self antigen on islet β cells and medullary thymic epithelial cells (mTECs) and a transgenic TCR recognizing the OVA323–339 peptide. CD4+CD40+ T cells and Treg cells each recognizing the cognate neo‐antigen, rather than being deleted through central tolerance, drastically expanded in the thymus. In pancreatic lymph nodes of DO11.RIPmOVA mice, CD4+CD40+ T cells and Treg cells are expanded in number compared with DO11 mice and importantly, Treg cells remain functional throughout the disease process. When exposed to neo‐self antigen, CD4+CD40+ T cells do not express the auto‐regulatory CTLA‐4 molecule while naïve CD4+CD40+ T cells do. DO11.RIPmOVA mice develop autoimmune‐type diabetes. CD40 engagement has been shown to prevent CTLA‐4 expression and injecting anti‐CD40 in DO11.RIPmOVA mice significantly exacerbates disease. These data suggest a unique means by which CD4+CD40+ T cells thwart tolerance.
Genetic variants in human leukocyte antigen/DP-DQ influence both hepatitis B virus clearance and hepatocellular carcinoma development
Authors: Hu Lingmin , Zhai Xiangjun , Liu Jibin , Chu Minjie , Pan Shandong , Jiang Jie , Zhang Yixin , Wang Hua , Chen Jianguo , Shen Hongbing , Hu Zhibin 
Published: November 2011

Abstract

Recent genome-wide association studies showed that four single-nucleotide polymorphisms (SNPs) in human leukocyte antigen (

HLA

)-
DP

(rs3077and rs9277535) and

HLA-DQ

(rs2856718 and rs7453920) were associated with chronic hepatitis B virus (HBV) infection in Japanese populations. More than 75% of hepatocellular carcinoma (HCC) patients are attributable to persistent infection of hepatitis B virus (HBV), especially in China. We genotyped these four SNPs in 1,300 HBV-positive HCC patients, 1,344 persistent HBV carriers, and 1,344 persons with HBV natural clearance from Southeast China to further test the associations of

HLA-DP/DQ

variants and with risk of both HBV clearance and HCC development. Logistic regression analyses showed that

HLA-DQ

rs2856718 significantly decreased host HCC risk, whereas three SNPs were associated with HBV clearance (

HLA-DP

rs9277535 as well as

HLA-DQ

rs7453920 and rs2856718). In addition,

HLA-DP

rs3077 showed an approaching significant effect on susceptibility to HBV persistent infection and HCC development when considering multiple testing adjustments. Taken together, we report, for the first time, that genetic variants in the

HLA-DP

and

HLA-DQ

loci may be marker SNPs for risk of both HBV clearance and HCC development. (Hepatology 2011)
Sixty-five gene-based risk score classifier predicts overall survival in hepatocellular carcinoma
Authors: Kim Soo Mi , Leem Sun-Hee , Chu In-Sun , Park Yun-Yong , Kim Sang Cheol , Kim Sang-Bae , Park Eun Sung , Lim Jae Yun , Heo Jeonghoon , Kim Yoon Jun , Kim Dae-Ghon , Kaseb Ahmed , Park Young Nyun , Wang Xin Wei , Thorgeirsson Snorri S. , Lee Ju-Seog 
Published: November 2011

Abstract

Clinical application of the prognostic gene expression signature has been delayed due to the large number of genes and complexity of prediction algorithms. In the current study we aimed to develop an easy-to-use risk score with a limited number of genes that can robustly predict prognosis of patients with hepatocellular carcinoma (HCC). The risk score was developed using Cox coefficient values of 65 genes in the training set (n = 139) and its robustness was validated in test sets (n = 292). The risk score was a highly significant predictor of overall survival (OS) in the first test cohort (

P

= 5.6 × 10−5, n = 100) and the second test cohort (

P

= 5.0 × 10−5, n = 192). In multivariate analysis, the risk score was a significant risk factor among clinical variables examined together (hazard ratio [HR], 1.36; 95% confidence interval [CI], 1.13-1.64;

P

= 0.001 for OS).

Conclusion:

The risk score classifier we have developed can identify two clinically distinct HCC subtypes at early and late stages of the disease in a simple and highly reproducible manner across multiple datasets. (Hepatology 2011)
Evaporative losses from soils covered by physical and different types of biological soil crusts
Authors: Chamizo S. , Cantón Y. , Domingo F. , Belnap J. 
Published: November 2011

AbstractEvaporation of soil moisture is one of the most important processes affecting water availability in semiarid ecosystems. Biological soil crusts, which are widely distributed ground cover in these ecosystems, play a recognized role on water processes. Where they roughen surfaces, water residence time and thus infiltration can be greatly enhanced, whereas their ability to clog soil pores or cap the soil surface when wetted can greatly decrease infiltration rate, thus affecting evaporative losses. In this work, we compared evaporation in soils covered by physical crusts, biological crusts in different developmental stages and in the soils underlying the different biological crust types. Our results show that during the time of the highest evaporation (Day 1), there was no difference among any of the crust types or the soils underlying them. On Day 2, when soil moisture was moderately low (11%), evaporation was slightly higher in well‐developed biological soil crusts than in physical or poorly developed biological soil crusts. However, crust removal did not cause significant changes in evaporation compared with the respective soil crust type. These results suggest that the small differences we observed in evaporation among crust types could be caused by differences in the properties of the soil underneath the biological crusts. At low soil moisture (<6%), there was no difference in evaporation among crust types or the underlying soils. Water loss for the complete evaporative cycle (from saturation to dry soil) was similar in both crusted and scraped soils. Therefore, we conclude that for the specific crust and soil types tested, the presence or the type of biological soil crust did not greatly modify evaporation with respect to physical crusts or scraped soils. Copyright © 2011 John Wiley & Sons, Ltd.
Prenatal diagnosis of Jeune‐like syndromes with two‐dimensional and three‐dimensional sonography
Authors: Rahmani Rose , Sterling Cara L. , Bedford H. Melanie 
Published: November 2011

AbstractThe purpose of this article is to describe the use of three‐dimensional sonography as an adjuvant to two‐dimensional sonography facilitating an earlier and more definitive diagnosis of Jeune and Jeune‐like syndromes in the second trimester. We report two cases in which three‐dimensional sonography facilitated the diagnosis of these malformations. A diagnosis of Jeune syndrome was made in our first case. Our second case was found to be short‐rib polydactyly syndrome Type IV. Three‐dimensional skeletal survey visualized short ribs, short limbs, the presence of normal scapulae, and the absence of polydactyly in both cases. Three‐dimensional sonography can assist two‐dimensional sonography in providing a more accurate display of skeletal anomalies, limb abnormalities, and facial features. © 2011 Wiley Periodicals, Inc. J Clin Ultrasound, 2011
Polyacetylene‐Type Networks Prepared by Coordination Polymerization of Diethynylarenes: New Type of Microporous Organic Polymers
Authors: Hanková Vladimíra , Slováková Eva , Zedník Jiří , Vohlídal Jiří , Sivkova Radoslava , Balcar Hynek , Zukal Arnošt , Brus Jiří , Sedláček Jan 
Published: November 2011

AbstractMicroporous organic polymers (MOP) of a new type have been synthesised in high yields by a simple coordination polymerization of 1,3‐diethynylbenzene, 1,4‐diethynylbenzene and 4,4′‐diethynylbiphenyl catalysed by [Rh(cod)acac] and [Rh(nbd)acac] complexes. The new MOPs are non‐swellable polyacetylene‐type conjugated networks consisting of ethynylaryl‐substituted polyene main chains that are crosslinked by arylene linkers. Prepared MOP samples have a mole fraction of branching units (by 13C CP/MAS NMR) from 0.30 to 0.47 and exhibit the BET (Brunaer‐Emmett‐Teller) surface up to 809 m2 g−1 and hydrogen uptake up to 0.69 wt% (77 K, H2 pressure 750 torr).
Consideration to minimize losses in terahertz coplanar waveguide on indium phosphide
Authors: Grimault‐Jacquin Anne‐Sophie , Tissafi Bouchra , Perret Etienne , Aniel Frédéric 
Published: November 2011

AbstractThe aim of this study is to analyze the losses of coplanar waveguide (CPW) in the Terahertz (THz) frequency range. A numerical analysis of the origin of losses (conductor, dielectric and radiation) for different geometries of the THz CPW on an Indium Phosphide substrate is presented. Two air‐bridges have been created over the central metallization and they are linked to the two ground planes in order to select the fundamental CPW mode by strongly reducing the unwanted slotline mode. Three numerical codes (HFSS, CST and an indigenously developed code called MAXTRA3D) are used for CPW modeling. Several CPW shapes are investigated showing that ohmic losses are dramatic for small central metallization‐ground spacing while radiation losses are dominating for large spacing. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:213–219, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26448
Compact and printed, standalone wireless wide area network antenna incorporating a conductive wire at low cost
Authors: Lee Cheng‐Tse , Su Saou‐Wen 
Published: November 2011

AbstractA compact and printed multiband antenna incorporating a conductive wire for wireless wide area network (WWAN) operation is presented. The antenna was formed on a low‐cost, single‐layered dielectric substrate of thickness 1 mm with the dimensions 10 × 55 mm2. The antenna is a couple‐fed short‐circuited monopole in the shape of T, which consists of two resonant paths for two wide operating bands. Unlike the conventional WWAN antennas utilizing a large system ground plane as the antenna ground to excite the antenna, the proposed antenna has a very small ground portion with comparable size to the radiating monopole and yet can also cover the GSM850/900 and GSM1800/1900/UMTS bands by incorporating a simply wire. The design is especially suited to some e‐book reader applications, in which the supporting metal frame for the E‐ink display is not required. In this case, the proposed antenna with long, narrow structure can be placed in the space between the casing and the display with the wire routed within the device. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:109–113, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26452
Broadband microstrip element design of A DBDP shared‐aperture sar array
Authors: Kong Ling‐Bing , Zhong Shun‐Shi , Sun Zhu 
Published: November 2011

AbstractA new S‐band element design of S/L‐band dual‐band dual‐polarized shared‐aperture array antenna for synthetic aperture radar applications is introduced, where the stacked patch structure is used to achieved broadband characteristics and especially the unsymmetrical crossed‐slots are adopted to improve the isolation and cross‐polarization performances both of the antenna element. By using this design with the antiphase feeding technique applied in the array, the array isolation of better than −45 dB is measured over the bandwidth 16.0%, while the measured array cross‐polarization level in 3dB beam width of −30 dB for vertical polarization and that of −28 dB for horizontal polarization are achieved. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:133–136, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26464
Novel NURBS‐PO method for efficiently analyzing antenna around an electrically large platform
Authors: He Zhi‐Li , Huang Kai , Liang Chang‐Hong 
Published: November 2011

AbstractA novel physics optics (PO) method based on nonuniform rational B spline (NURBS) modeling is used to calculate the disturbed pattern of an antenna around an electrically large platform.Compared with the conventional NURBS‐PO method, in which the true scattered field of an antenna is replaced by the spherical wave, the new technique uses the accurate radiation field as the incident field and overcomes the limitation that the antenna must be located far away from the scatterer. In addition, the computational efficiency is significantly improved using interpolation of critical points. Two numerical examples are calculated. Compared with the results calculated by other algorithms, such as method of moments and conventional NURBS‐PO, the proposed method shows great capability to solve this particular problem precisely and efficiently. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:64–66, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26467
Microstrip antenna perturbed by a defected ground structure with a reconfigurable polarization capability
Authors: Pyo Seongmin , Sung Youngje 
Published: November 2011

AbstractIn this article, a novel square microstrip antenna with a reconfigurable polarization capability is proposed that utilizes a perturbing segment in a defected ground structure (DGS).To verify the perturbing method, DGSs were embedded into either the two diagonal or all four corners of an antenna. The reconfigurable characteristics are produced by independently controlling the states of the biased DGS PIN diodes. The simulated and measured results show good frequency agility and polarization diversity with remarkable radiation performances. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:58–61, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26485
Enhanced bandwidth small E‐Shaped monopole antenna for UWB applications with variable frequency band‐notch function
Authors: Karimi H. , Ghobadi Ch. , Nourinia J. , Ojaroudi M. 
Published: November 2011

AbstractIn this article, a novel printed monopole antenna for ultra wideband applications with variable frequency band‐notch characteristic is presented.The proposed antenna consists of an E‐shaped radiating patch with two L‐shaped arms and a T‐shaped arm which provides a wide usable fractional bandwidth of more than 130% (2.9–14 GHz). By inserting two modified L‐shaped arms with variable dimensions on the E‐shaped radiating patch frequency band‐stop performance is generated and also by inserting T‐shaped arm this performance is improved. We can control band‐stop performance characteristics such as band‐notch frequency and its bandwidth. The designed antenna has a small size of 10 × 17 mm2 while showing the band rejection performance in the frequency band of 5.01–5.98 GHz. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:267–271, 2012 View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26487
Ultra‐wideband small square monopole antenna with variable frequency notch band characteristics using an interdigital slot
Authors: Zolfagharian A. R. , Azarmanesh M. N. , Ojaroudi M. 
Published: November 2011

AbstractIn this article, a novel printed monopole antenna for ultra‐wideband applications with variable frequency band‐notch characteristic is presented.The proposed antenna consists of a square radiating patch with a modified interdigital slot and a ground plane with a pair of inverted L‐shaped sleeves which provides a wide usable fractional bandwidth of more than 130% (2.9–14.6 GHz). By cutting a modified interdigital slot with variable dimensions on the radiating patch frequency band‐stop performance is generated, and we can control its characteristics such as band‐notch frequency and its bandwidth. The designed antenna has a small size of 10 × 16 mm2 while showing the band rejection performance in the frequency band of 5.04–5.96 GHz. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26488
Power‐dependent conductivity of electrolyte aqueous solutions under microwave irradiation at 915 MHz: An experimental study
Authors: Xiaoqing Yang , Shang H. , Huacheng Zhu , Ke Li , Kama Huang 
Published: November 2011

AbstractThe reflection coefficients from a gold‐plated coaxial line probe submerged in different electrolyte aqueous solutions are measured under different microwave powers and constant temperature. Measurements were performed at 915 MHz. Furthermore, a GA‐based inverse calculation technique was formulated and used to determine the effective conductivity of the solution from the measured reflection coefficients. A new phenomenon was observed: the effective conductivity of electrolyte aqueous solutions can be directly influenced by microwave power. Any thermal effect was excluded using multiphysics calculation and temperature measurement during the experiment for sodium chloride aqueous solution. Therefore, it was concluded that nonthermal effect of sodium chloride aqueous solution under microwave radiation exists. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:222–225, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26500
A dual‐resonance injection‐locked frequency doubler in 0.18 μm CMOS technology
Authors: Jang Sheng‐Lyang , Chang Chia‐Wei , Huang Chong‐Wei , Hsue Ching‐Wen 
Published: November 2011

AbstractThis article proposes a dual‐resonance CMOS LC‐tank injection‐locked frequency doubler (ILFD) fabricated in the 0.18 μm CMOS process and describes the circuit design, operation principle, and measurement results of the ILFD. The ILFD circuit is composed of a dual‐resonance first‐harmonic injection‐locked oscillator with dual‐injection ports, a wide‐band frequency doubler, and a transformer balun. At the supply voltage of 0.7 V, the dc power consumption is 5.39 mW. At the incident power of 0 dBm, the ILFD has high/low operation range from the incident frequency 3.9/1.7 to 6.1/2 GHz to provide a dual‐band signal source with the frequency 7.8/3.4–12.2/4 GHz. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:193–196, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26501
Split augmented hammerstein model with neural network for a three‐stage doherty power amplifier
Authors: Lee Mun‐Woo , Kam Sang‐Ho , Jeong Yoon‐Ha 
Published: November 2011

AbstractThis article describes a split augmented Hammerstein (SAH) model with a neural network (NN) for the three‐stage Doherty power amplifier (DPA). The NN in the memoryless system is used to characterize the severe nonlinear changes in the magnitude and phase distortions. Several finite impulse response filters in the memory system are used to represent the memory effects of the DPA. The three‐stage DPA implemented by Si LDMOSFETs is used to validate the performance of the proposed model. For a 2‐FA wideband code division multiple access (WCDMA) signal with 10‐MHz carrier spacing, the experimental results show that the proposed model characterizes not only the inconsistent changes in the static nonlinearity, but also the memory effects of the three‐stage DPA. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:124–126, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26517
A discrete element approach to elastic properties of layered geomaterials
Authors: Buechler Scott R. , Berger John R. , Mustoe Graham G. W. 
Published: November 2011

SUMMARYDiscrete element methods (DEMs) are used for layered geomaterials to investigate the dependency of traditional engineering constants on material properties and loading conditions. Shear deformations and compression tests parallel and perpendicular to layering are conducted on samples of varying kerogen volume fractions, confining pressures, porosities, and layer geometries. The goal of this article is to develop a method to better characterize oil shale (a transversely isotropic layered geomaterial) while eliminating high experimental costs. The DEM simulations conducted in this study demonstrate strong dependencies of Young's modulus, Poisson's ratio, and shear modulus on kerogen volume fraction and porosity. Furthermore, a rule of thumb for layer thickness and particle resolution is proposed for simulation design. Results agree well with robust effective medium theories, solidify the ability of DEM to model the mechanical properties of layered heterogenous materials, and encourage the use of DEM to study more complicated layered media and material failure. Copyright © 2011 John Wiley & Sons, Ltd.