bluuebunny/crossref_metadata_2025_split huggingface dataset:
Successful percutaneous retrieval of methyl methacrylate orthopedic cement embolism from the pulmonary artery
Authors: Bose Rahul , Choi James W. 
Published: February 2010

AbstractVertebroplasty cement embolization into the venous system has long been recognized as a potential complication, but the true incidence of systemic embolization is unknown. Clinical presentations range from patients who are asymptomatic or have incidental findings on imaging to massive pulmonary embolism resulting in death. Optimal treatment is controversial and the natural history is unknown. We present the case of an 85‐year‐old female undergoing combined laminectomy and vertebroplasty with subsequent pulmonary embolism of the cement which was successfully retrieved from a percutaneous approach. © 2010 Wiley‐Liss, Inc.
Reproducibility of near‐infrared spectroscopy for the detection of lipid core coronary plaques and observed changes after coronary stent implantation
Authors: Garcia Bobby A. , Wood Frances , Cipher Daisha , Banerjee Subhash , Brilakis Emmanouil S. 
Published: February 2010

AbstractObjectives: To evaluate the reproducibility of catheter‐based intracoronary near‐infrared spectroscopy (NIRS) for the detection of lipid core coronary plaques (LCPs) and to examine stenting‐induced changes in NIRS findings. Background: The in‐vivo reproducibility of coronary NIRS findings and their changes after stenting have not previously been characterized. Methods: NIRS assessment using an automated pullback catheter was performed in duplicate in 36 vessels in 31 patients. The reproducibility of the Lipid Core Burden Index (LCBI) and the presence and number of LCPs was assessed. The changes in LCBI after stenting were also assessed in 25 vessels in 22 patients. Results: LCBI of the first and second pullback was 64 ± 43 and 70 ± 62, respectively, with excellent correlation (Spearman's rho 0.927, intraclass correlation coefficient 0.925). Depending on LCP definition, mean LCP length, and median LCP number ranged from 2.44 to 17.25 mm, and from 0 to 2, respectively per artery studied. High correlation was observed between the two pullbacks for total LCP length (depending on the LCP definition used, the Spearman's rho and the intraclass correlation coefficient ranged from 0.72 to 0.89, and from 0.76 to 0.91, respectively) and for LCP number (depending on the LCP definition used, the Spearman's rho and the intraclass correlation coefficient ranged from 0.70 to 0.87, and from 0.67 to 0.88, respectively). The mean LCBI decreased by 40% from 78 ± 47 to 48 ± 38 after stenting (P < 0.001). Conclusion: The LCBI and LCP length NIRS measurements have high reproducibility. LCBI significantly decreases after coronary stenting. © 2010 Wiley‐Liss, Inc.
The Construction of Open GdIII Metal–Organic Frameworks Based on Methanetriacetic Acid: New Objects with an Old Ligand
Authors: Cañadillas‐Delgado Laura , Martín Tomás , Fabelo Oscar , Pasán Jorge , Delgado Fernando S. , Lloret Francesc , Julve Miguel , Ruiz‐Pérez Catalina 
Published: February 2010

AbstractThe preparation, X‐ray crystallography and magnetic investigation of the first examples of methanetriacetate (mta)‐containing lanthanide(III) complexes of formulae [Gd(mta)(H2O)3]n⋅4 n H2O (1) [Gd(mta)(H2O)3]n⋅2 n H2O (2) and [Gd2(mta)2(H2O)2]n⋅2 n H2O (3) are described herein. This tripodal ligand promotes the formation of 63 networks; thus 1 consists of a honeycomb structure, whereas in 2 two of these layers are condensed to form a rare five‐connected two‐dimensional (4862) network. Compound 3 can be seen as an aggregation of 63 layers leading to a three‐dimensional (6,6)‐connected binodal (41263)(4966)‐nia net, in which the gadolinium(III) ions and the mta ligands act as octahedral and as trigonal prismatic nodes, respectively. The magnetic properties of 1–3 were investigated in the temperature range 1.9–300 K. A close fit to the Curie law (1) and weak either antiferro‐ [J=−0.0063(1) cm−1 (2)] or ferromagnetic [J=+0.0264(6) cm−1 (3)] interactions between the GdIII ions are observed; the different exchange pathways involved [extended tris‐bidentate mta (1) and μ‐O(1);κ2O(1),O(2) (2 and 3) plus single syn–syn carboxylate‐mta (3)] accounting for these magnetic features. The nature and magnitude of the magnetic interactions, between the GdIII ions in 1–3, agree with the small amount of data existing in the literature for these kind of bridges.
Peripheral Arterial Tonometry for Risk Stratification in Men With Coronary Artery Disease
Authors: Heffernan Kevin S. , Karas Richard H. , Patvardhan Eshan A. , Jafri Haseeb , Kuvin Jeffrey T. 
Published: February 2010

AbstractBackgroundCoronary artery disease (CAD) risk is not fully revealed by traditional risk factors. Identification of a simple, noninvasive tool that allows for detection of high‐risk CAD patients and can be applied in large populations and clinical settings would prove valuable.HypothesisWe sought to test the hypothesis that peripheral arterial tonometry (PAT) would be associated with residual risk in men with CAD.MethodsIn this study, finger PAT was used to measure pulse wave amplitude (PWA) during reactive hyperemia (RH) and taken as a measure of microvascular endothelial function in 42 men with stable CAD and well controlled low‐density lipoprotein cholesterol (LDL‐C) levels. Plasma levels of high‐sensitivity C‐reactive protein (hs‐CRP) and lipoprotein‐associated phospholipase A2 (Lp‐PLA2) were measured and used to reclassify men into high‐risk (elevated hs‐CRP and Lp‐PLA2), moderate‐risk (either elevated hs‐CRP or Lp‐PLA2), or low‐risk (low hs‐CRP and Lp‐PLA2) groups.ResultsPWA‐RH was significantly lower in the high‐risk group (1.3 ± 0.04) compared to the moderate‐risk (1.6 ± 0.07, P < 0.05) and low‐risk (2.0 ± 0.1, P < 0.05) groups. According to binary logistic regression, PWA‐RH was a significant predictor of high‐risk status among men with CAD (P < 0.05).ConclusionMeasurement of peripheral microvascular endothelial function with PAT may be able to distinguish high‐risk men from moderate‐ and low‐risk men with stable CAD and well‐controlled LDL‐C levels and thus aid in residual risk stratification in this at risk cohort. Copyright © 2010 Wiley Periodicals, Inc.
A Left Hemiblock Improves Cardiac Resynchronization Therapy Outcomes in Patients With a Right Bundle Branch Block
Authors: Chandra Ramesh , Zolty Ronald , Palma Eugen 
Published: February 2010

AbstractBackgroundThe current recommendation for cardiac resynchronization therapy (CRT) in congestive heart failure (CHF) patients is based on QRS duration, not on QRS morphology.HypothesisThis study was performed to compare the effects of CRT in CHF patients with pure RBBB vs those with a coexisting left hemiblock (LHB).Methods and Patient PopulationA total of 271 consecutive patients who underwent CRT at Montefiore Medical Center were analyzed. Baseline ECGs were analyzed by 2 reviewers for RBBB and further classified into those with a coexisting LHB. Response to CRT was defined to be, at ≥ 6 months after CRT, either an improvement in ejection fraction (EF) of at least 5%, or an improvement in New York Heart Association (NYHA) CHF class. A total of 44 patients were identified: 18 had pure RBBB and 26 had a coexisting LHB. The 2 groups were similar in respect to baseline characteristics (P > 0.05).ResultsOnly 4 out of 18 patients with pure RBBB compared to 18 out of 26 with LHB (P = 0.005) had an improvement in EF ≥ 5%. The mean EF was − 1% in the pure RBBB group, but + 5.4% in those with LHB (P = 0.0031). Improvement in NYHA class was seen in 0 out of 18 with pure RBBB vs 7 out of 26 patients with LHB (P = 0.03).ConclusionIf patients with RBBB also had LHB, their response to CRT was significantly better than if they had RBBB alone. Copyright © 2010 Wiley Periodicals, Inc.
H2 Conversion in the Presence of O2 as Performed by the Membrane‐Bound [NiFe]‐Hydrogenase of Ralstonia eutropha
Authors: Lenz Oliver , Ludwig Marcus , Schubert Torsten , Bürstel Ingmar , Ganskow Stefanie , Goris Tobias , Schwarze Alexander , Friedrich Bärbel 
Published: February 2010

Abstract[NiFe]‐hydrogenases catalyze the oxidation of H2 to protons and electrons. This reversible reaction is based on a complex interplay of metal cofactors including the Ni–Fe active site and several [Fe–S] clusters. H2 catalysis of most [NiFe]‐hydrogenases is sensitive to dioxygen. However, some bacteria contain hydrogenases that activate H2 even in the presence of O2. There is now compelling evidence that O2 affects hydrogenase on three levels: 1) H2 catalysis, 2) hydrogenase maturation, and 3) H2‐mediated signal transduction. Herein, we summarize the genetic, biochemical, electrochemical, and spectroscopic properties related to the O2 tolerance of hydrogenases resident in the facultative chemolithoautotroph Ralstonia eutropha H16. A focus is given to the membrane‐bound [NiFe]‐hydogenase, which currently represents the best‐characterized member of O2‐tolerant hydrogenases.
CD122+CD8+ Treg suppress vaccine‐induced antitumor immune responses in lymphodepleted mice
Authors: Wang Li‐Xin , Li Yuhuan , Yang Guojun , Pang Pui‐yi , Haley Dan , Walker Edwin B. , Urba Walter J. , Hu Hong‐Ming 
Published: February 2010

AbstractLymphodeleption prior to adoptive transfer of tumor‐specific T cells greatly improves the clinical efficacy of adoptive T‐cell therapy for patients with advanced melanoma, and increases the therapeutic efficacy of cancer vaccines in animal models. Lymphodepletion reduces competition between lymphocytes, and thus creates “space” for enhanced expansion and survival of tumor‐specific T cells. Within the lymphodepleted host, Ag‐specific T cells still need to compete with other lymphocytes that undergo lymphopenia‐driven proliferation. Herein, we describe the relative capacity of naïve T cells, Treg, and NK cells to undergo lymphopenia‐driven proliferation. We found that the major population that underwent lymphopenia‐driven proliferation was the CD122+ memory‐like T‐cell population (CD122+CD8+ Treg), and these cells competed with Ag‐driven proliferation of melanoma‐specific T cells. Removal of CD122+CD8+ Treg resulted in a greater expansion of tumor‐specific T cells and tumor infiltration of functional effector/memory T cells. Our results demonstrate the lymphopenia‐driven proliferation of CD122+CD8+ Treg in reconstituted lymphodepleted mice limited the antitumor efficacy of DC vaccination in conjunction with adoptive transfer of tumor‐specific T cells.
Regulatory role of leukocyte common antigen‐related molecule (LAR) in thymocyte differentiation
Authors: Kondo Sachiko , Kishi Hiroyuki , Muraguchi Atsushi 
Published: February 2010

AbstractThe strength of interaction between the antigenic peptide‐loaded MHC (MHC/p) and the TCR determines T‐cell fate in the thymus. A high avidity interaction between the TCR and the MHC/p induces apoptosis of self‐reactive T cells (negative selection), whereas a moderate avidity interaction rescues thymocytes from apoptosis and permits further differentiation to mature T cells (positive selection). Leukocyte common antigen‐related molecule (LAR), a receptor‐like protein tyrosine phosphatase, is expressed on immature thymocytes, but its role in thymocyte differentiation has not yet been fully elucidated. We analyzed LAR‐deficient mice and demonstrated that LAR deficiency affected the differentiation and expansion of immature thymocytes as well as positive and negative selection. Furthermore, LAR deficiency resulted in a lower Ca2+ response. The results indicate that LAR is an important modulator of TCR signaling that controls thymocyte differentiation.
Synthesis of Phosphanyl Sulfoximines Through Phospha‐Michael Reaction of Alkenyl Sulfoximines and Their Evaluation as Chiral Bidentate 1,5‐N,P Ligands for Palladium in Asymmetric Allylic Alkylation
Authors: Lemasson Fabien , Gais Hans‐Joachim , Runsink Jan , Raabe Gerhard 
Published: February 2010

AbstractThe intermolecular phospha‐Michael reaction of cyclic and acyclic alkenyl sulfoximines proceeds readily and yields the corresponding phosphanyl sulfoximines in good yield. The asymmetric induction provided by sulfoximine group in C–P bond formation is apparently only low. The configuration of three phosphanyl sulfoximine–boranes has been determined by X‐ray crystal structure analysis. The ability of the phosphanyl sulfoximines to act as ligand in Pd‐catalyzed asymmetric allylic alkylation was studied with pairs of diastereomeric cyclic and acyclic derivatives carrying different substituents at the N atom. This study showed that the substituent at the N atom and both the chirality of the backbone and sulfoximine group play a crucial role in determining the enantioselectivity of the allylic alkylation. The Pd‐catalyzed reaction of the racemic 1,3‐diphenylallyl acetate with the malonate anion in the presence of a cyclic N‐benzyl‐substituted phosphanyl sulfoximine gives the corresponding malonate with 97 % ee in 98 % yield. The similar alkylation with dialkyl‐substituted allylic acetates proceeds only with medium enantioselectivity. The bidentate 1,5‐N,P‐coordination of the Pd atom by the phosphanyl sulfoximine was confirmed by NMR experiments of a π‐1,3‐diphenylallyl‐PdII complex containing a cyclohexyl phosphanyl sulfoximine as ligand. The cyclohexane ring of the free cyclic phosphanyl sulfoximines adopts in solution and in the crystal a conformation in which the sulfoximine and phosphanyl group are both in a pseudo axial position. The coordination of the ligand to the Pd atom causes an inversion of the cyclohexane ring, which places the two groups in equatorial position.
Generation of Nucleophilic Chromium Acetylides from gem‐Trichloroalkanes and Chromium Chloride: Synthesis of Propargyl Alcohols
Authors: Kashinath Dhurke , Tisserand Steve , Puli Narender , Falck John R. , Baati Rachid 
Published: February 2010

AbstractNucleophilic mixed chromium(II) and chromium(III) acetylides are generated from the smooth reduction of primary 1,1,1‐trichloroalkanes with chromium(II) chloride in the presence of an excess amount of triethylamine at room temperature. These species arise from chromium(III) vinylidene carbenoids. It has been demonstrated that uncommon low‐valent CrII acetylides are formed by C–H insertion of CrIICl2 into terminal alkynes, formed in situ through the Fritsch–Buttenberg–Wiechell (FBW) rearrangement, whereas CrIII acetylides are concomitantly generated by HCl elimination from the chromium(III) vinylidene carbenoid. Both divergent pathways result, overall, in the formation of nucleophilic acetylides. In situ trapping with electrophilic aldehydes afforded propargyl alcohols. Furthermore, deuteration experiments and the use of deuterium labeled 1,1,1‐trichloroalkane substrates demonstrated the prevalence of low‐valent CrII acetylides, potentially useful, yet highly elusive synthetic intermediates.
NPA motifs play a key role in plasma membrane targeting of aquaporin‐4
Authors: Guan Xin‐Gang , Su Wei‐Heng , Yi Fei , Zhang Di , Hao Feng , Zhang Hong‐Guo , Liu Yue‐Jiao , Feng Xue‐Chao , Ma Tong‐Hui 
Published: February 2010

AbstractThe two highly conserved NPA motifs (asparagine–proline–alanine, NPA) are the most important structural domains that play a crucial role in water‐selective permeation in aquaporin water channels. However, the functions of NPA motifs in aquaporin (AQP) biogenesis remain largely unknown. Few AQP members with variations in NPA motifs such as AQP11 and AQP12 do not express in the plasma membrane, suggesting an important role of NPA motifs in AQP plasma membrane targeting. In this study, we examined the role of the two NPA motifs in AQP4 plasma membrane targeting by mutagenesis. We constructed a series of AQP4 mutants with NPA deletions or single amino acid substitutions in AQP4‐M1 and AQP4‐M23 isoforms and analyzed their expression patterns in transiently transfected FRT and COS‐7 cells. Western blot analysis showed similar protein bands of all the AQP4 mutants and the wild‐type AQP4. AQP4 immunofluorescence indicated that deletion of one or both NPA motifs resulted in defective plasma membrane targeting, with apparent retention in endoplasmic reticulum (ER). The A99T mutant mimicking AQP12 results in ER retention, whereas the A99C mutant mimicking AQP11 expresses normally in plasma membrane. Furthermore, the AQP4‐M1 but not the M23 isoform with P98A substitution in the first NPA motif can target to the plasma membrane, indicating an interaction of N‐terminal sequence of AQP4‐M1 with the first NPA motif. These results suggest that NPA motifs play a key role in plasma membrane expression of AQP4 but are not involved in AQP4 protein synthesis and degradation. The NPA motifs may interact with other structural domains in the regulation of membrane trafficking during aquaporin biogenesis. © 2010 IUBMB IUBMB Life, 62(3): 222–226, 2010
Urinary levels, tissue concentrations and lung inflammation after nose‐only exposure to three different chemical forms of beryllium
Authors: Muller Caroline , Mazer Bruce , Salehi Fariba , Audusseau Séverine , Chevalier Gaston , Truchon Ginette , Larivière Pierre , Paquette Vincent , Lambert Jean , Zayed Joseph 
Published: February 2010

AbstractThis study aimed to determine the toxicity and toxicokinetic of three Be chemical species A total of 120 mice (four groups of 30) were nose‐only exposed. The first group was used as a control while the three others were exposed to 250 μg m−3 of fine particles of three different Be species (Be metal, Be–F; Be oxide, BeO–F; Be aluminium, BeAl–F). Exposure lasted over three consecutive weeks, five days per week and 6 h per day. Blood and several tissues were collected one week after exposure. Urines were collected before the beginning of exposure, at the end of every week of exposure and one week after exposure. Results showed that urine concentrations were different from one Be species to another and that excretion continued after the end of exposure. Except for BeO–F, where Be urine concentrations were stable during the three weeks of exposure, concentrations of Be–F and BeAl–F reached a peak after the first week. According to particle size, BeO–F obtained the highest theoretical pulmonary deposition rate, which partially led to the highest Be lung concentration. This group also presented the lowest urine concentration but that did not lead to more severe lung inflammation. Moreover, even if BeAl–F obtained the lowest percentage theoretical pulmonary deposition, it showed the highest Be urinary concentration, the lowest Be lung concentration and the lowest lung toxicity. In this specific case, a high Be concentration in urine did not reflect a high exposure or a severe toxic effect. Copyright © 2010 John Wiley & Sons, Ltd.
3D collagen scaffolds coated with multiwalled carbon nanotubes: Initial cell attachment to internal surface
Authors: Hirata Eri , Uo Motohiro , Nodasaka Yoshinobu , Takita Hiroko , Ushijima Natsumi , Akasaka Tsukasa , Watari Fumio , Yokoyama Atsuro 
Published: February 2010

AbstractThe cell adhesion in a multiwalled carbon nanotube‐coated collagen sponge (MWCNT‐coated sponge) was investigated. Immediately after seeding, the cells adhered to the inner surface of the MWCNT‐coated sponge and a significantly larger number of cells were observed there than for a pure collagen sponge used as control. On the MWCNT‐coated sponge, the cells appeared favorable adhesion and spread in the early stages in the center part of the sponge which cells rarely attached without MWCNT‐coating. It was suggested that the physical structure of MWCNTs was effective for initial adhesion of cells from the result of serum‐free culture. MWCNT‐coating makes the material a suitable three‐dimensional scaffold for cell culturing, as opposed to other scaffold systems where such an effect is not seen. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Microindentation for in vivo measurement of bone tissue mechanical properties in humans
Authors: Diez-Perez Adolfo , Güerri Roberto , Nogues Xavier , Cáceres Enric , Peña Maria Jesus , Mellibovsky Leonardo , Randall Connor , Bridges Daniel , Weaver James C , Proctor Alexander , Brimer Davis , Koester Kurt J , Ritchie Robert O , Hansma Paul K 
Published: February 2010

Abstract
Bone tissue mechanical properties are deemed a key component of bone strength, but their assessment requires invasive procedures. Here we validate a new instrument, a reference point indentation (RPI) instrument, for measuring these tissue properties in vivo. The RPI instrument performs bone microindentation testing (BMT) by inserting a probe assembly through the skin covering the tibia and, after displacing periosteum, applying 20 indentation cycles at 2 Hz each with a maximum force of 11 N. We assessed 27 women with osteoporosis-related fractures and 8 controls of comparable ages. Measured total indentation distance (46.0 ± 14 versus 31.7 ± 3.3 µm, p = .008) and indentation distance increase (18.1 ± 5.6 versus 12.3 ± 2.9 µm, p = .008) were significantly greater in fracture patients than in controls. Areas under the receiver operating characteristic (ROC) curve for the two measurements were 93.1% (95% confidence interval [CI] 83.1–100) and 90.3% (95% CI 73.2–100), respectively. Interobserver coefficient of variation ranged from 8.7% to 15.5%, and the procedure was well tolerated. In a separate study of cadaveric human bone samples (n = 5), crack growth toughness and indentation distance increase correlated (r = –0.9036, p = .018), and scanning electron microscope images of cracks induced by indentation and by experimental fractures were similar. We conclude that BMT, by inducing microscopic fractures, directly measures bone mechanical properties at the tissue level. The technique is feasible for use in clinics with good reproducibility. It discriminates precisely between patients with and without fragility fracture and may provide clinicians and researchers with a direct in vivo measurement of bone tissue resistance to fracture. © 2010 American Society for Bone and Mineral Research
GADD45α mediates arsenite‐induced cell apoptotic effect in human hepatoma cells via JNKs/AP‐1‐dependent pathway
Authors: Gao Ming , Dong Wen , Hu Meiru , Yu Ming , Guo Liang , Qian Lu , Guo Ning , Song Lun 
Published: February 2010

AbstractArsenite (As(III)), an effective chemotherapeutic agent for the acute promyelocytic leukemia (APL) and multiple myeloma (MM), might be also a promise for the therapy of other cancers, including the solid tumors. However, the molecular bases of arsenite‐induced cytotoxicity in the tumor cells have not been fully defined. In this study, we have disclosed that arsenite effectively induces the apoptotic response in the HepG2 human hepatoma cells by triggering GADD45α induction and the subsequent activation of JNKs/AP‐1 cell death pathway. However, signaling events relating to GADD45α/JNKs/AP‐1 pathway activation have not been observed in HL7702 human diploid hepatic cells under the same arsenite exposure condition. Our results thus have illustrated the selective pro‐apoptotic role of arsenite in the hepatoma cells by activating GADD45α‐dependent cell death pathway whereas with little effect on the normal hepatic cells. The approaches to up‐regulate GADD45α levels might be helpful in improving the chemotherapeutic action of arsenite on certain solid tumors including hepatoma. J. Cell. Biochem. 109: 1264–1273, 2010. © 2010 Wiley‐Liss, Inc.
A simple synthesis of pyrroles catalyzed by acidic resin under solvent‐free condition
Authors: Yuan Shizhen , Li Zhen , Xu Ling 
Published: February 2010

Abstract
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A simple and effective Paal‐Knorr condensation of 2, 5‐hexanedione with most amines has been carried out at room temperature under solvent‐free condition. The pyrroles were obtained in high yields and in short reaction times. J. Heterocyclic Chem., (2010).