bluuebunny/crossref_metadata_2025_split huggingface dataset:
Learned trustworthiness does not have the same influence on implicit responses measured via fast periodic visual stimulation as face trustworthiness
Authors: Verosky Sara C. , Nishiura Hannah , O'Brien Lucie , Liu Huanting , Aggadi Nada 
Published: May 2024

AbstractPast research has demonstrated that it is possible to detect implicit responses to face trustworthiness using fast periodic visual stimulation (FPVS). Because people readily retrieve affective associations with faces, the current study investigated whether learned trustworthiness would yield similar responses to face trustworthiness as measured via FPVS. After learning to associate faces with untrustworthy or trustworthy behaviors, participants completed three separate tasks while electroencephalography (EEG) was recorded. In each of these tasks, participants viewed oddball sequences of faces where a single base face was presented repeatedly at a rate of 6 Hz and oddball faces with different identities were presented every fifth face (6 Hz/5 = 1.2 Hz). Providing evidence of learning, the oddball response at 1.2 Hz and its harmonics was stronger for the learned faces compared to novel faces over bilateral occipitotemporal cortex and beyond. In addition, reproducing previous findings with face trustworthiness, we observed a stronger response at 1.2 Hz and its harmonics for sequences with less trustworthy‐looking versus trustworthy‐looking oddball faces over bilateral occipitotemporal cortex and other sites. However, contrary to our predictions, we did not observe a significant influence of learned trustworthiness on the oddball response. These data indicate that impressions based on learning are treated differently than impressions based on appearance, and they raise questions about the types of design and stimuli that yield responses that are measurable via FPVS.
The rise in cross‐national marriages and the emergent inequalities in East and Southeast Asia
Authors: Lu Shuya , Yeung Wei‐Jun Jean 
Published: May 2024

AbstractThis paper reviews the trends and driving forces for the relatively recent growth in cross‐national marriages in East and Southeast Asia with a specific focus on the experiences of female marriage migrants from Southeast Asia. It explicates the various forms of inequality faced by the low‐income marriage migrant women including gender power dynamics within the household, socioeconomic inequalities, integration into the destination society, and intergenerational transfer of inequalities. We acknowledge that the inequality experienced by marriage migrant women results from a complex web of intersecting social‐cultural, political, and economic forces occurring in Asia in the past three decades. As a result of rising female educational attainment and the practice of marriage hypergamy in Asia, an increasing number of low socioeconomic‐status men from developed countries seek marriage partners abroad from lower‐income and less‐developed countries. Upon entering the cross‐national marriage market, many low‐income migrant women face multiple disadvantages in the host country due to inadequate socioeconomic support, language barriers, social stigma on foreign brides, and a lack of legal status. These inequalities exacerbate their initial disadvantages to perpetuate a vicious cycle of intergenerational disadvantages for their offspring. We conclude with a critique of extant literature and identify promising future research directions.
Enhancing genome‐wide populus trait prediction through deep convolutional neural networks
Authors: Duan Huaichuan , Dai Xiangwei , Shi Quanshan , Cheng Yan , Ge Yutong , Chang Shan , Liu Wei , Wang Feng , Shi Hubing , Hu Jianping 
Published: May 2024

SUMMARYAs a promising model, genome‐based plant breeding has greatly promoted the improvement of agronomic traits. Traditional methods typically adopt linear regression models with clear assumptions, neither obtaining the linkage between phenotype and genotype nor providing good ideas for modification. Nonlinear models are well characterized in capturing complex nonadditive effects, filling this gap under traditional methods. Taking populus as the research object, this paper constructs a deep learning method, DCNGP, which can effectively predict the traits including 65 phenotypes. The method was trained on three datasets, and compared with other four classic models—Bayesian ridge regression (BRR), Elastic Net, support vector regression, and dualCNN. The results show that DCNGP has five typical advantages in performance: strong prediction ability on multiple experimental datasets; the incorporation of batch normalization layers and Early‐Stopping technology enhancing the generalization capabilities and prediction stability on test data; learning potent features from the data and thus circumventing the tedious steps of manual production; the introduction of a Gaussian Noise layer enhancing predictive capabilities in the case of inherent uncertainties or perturbations; fewer hyperparameters aiding to reduce tuning time across datasets and improve auto‐search efficiency. In this way, DCNGP shows powerful predictive ability from genotype to phenotype, which provide an important theoretical reference for building more robust populus breeding programs.
Rhamnogalacturonan lyase 1 (RGL1), as a suppressor of E3 ubiquitin ligase Arabidopsis thaliana ring zinc finger 1 (AtRZF1), is involved in dehydration response to mediate proline synthesis and pectin rhamnogalacturonan‐I composition
Authors: Min Ji‐Hee , Park Cho‐Rong , Gong Ying , Chung Moon‐Soo , Nam Seung‐Hee , Yun Hye Sup , Kim Cheol Soo 
Published: May 2024

SUMMARYProline metabolism plays a crucial role in both environmental stress responses and plant growth. However, the specific mechanism by which proline contributes to abiotic stress processes remains to be elucidated. In this study, we utilized atrzf1 (Arabidopsis thaliana ring zinc finger 1) as a parental line for T‐DNA tagging mutagenesis and identified a suppressor mutant of atrzf1, designated proline content alterative 31 (pca31). The pca31 mutant suppressed the insensitivity of atrzf1 to dehydration stress during early seedling growth. Using Thermal Asymmetric Interlaced‐PCR, we found that the T‐DNA of pca31 was inserted into the promoter region of the At2g22620 gene, which encodes the cell wall enzyme rhamnogalacturonan lyase 1 (RGL1). Enzymatic assays indicated that RGL1 exhibited rhamnogalacturonan lyase activity, influencing cell wall pectin composition. The decrease in RGL1 gene expression suppressed the transcriptomic perturbation of the atrzf1 mutant. Silencing of the RGL1 gene in atrzf1 resulted in a sensitive phenotype similar to pca31 under osmotic stress conditions. Treatment with mannitol, salt, hydrogen peroxide, and abscisic acid induced RGL1 expression. Furthermore, we uncovered that RGL1 plays a role in modulating root growth and vascular tissue development. Molecular, physiological, and genetic experiments revealed that the positive modulation of RGL1 during abiotic stress was linked to the AtRZF1 pathway. Taken together, these findings establish that pca31 acts as a suppressor of atrzf1 in abiotic stress responses through proline and cell wall metabolisms.
Role of NaV1.7 in postganglionic sympathetic nerve function in human and guinea‐pig arteries
Authors: Kim Joyce S. , Meeker Sonya , Ru Fei , Tran Minh , Zabka Tanja S. , Hackos David , Undem Bradley J. 
Published: May 2024

AbstractNaV1.7 plays a crucial role in inducing and conducting action potentials in pain‐transducing sensory nociceptor fibres, suggesting that NaV1.7 blockers could be effective non‐opioid analgesics. While SCN9A is expressed in both sensory and autonomic neurons, its functional role in the autonomic system remains less established. Our single neuron rt‐PCR analysis revealed that 82% of sympathetic neurons isolated from guinea‐pig stellate ganglia expressed NaV1.7 mRNA, with NaV1.3 being the only other tetrodotoxin‐sensitive channel expressed in approximately 50% of neurons. We investigated the role of NaV1.7 in conducting action potentials in postganglionic sympathetic nerves and in the sympathetic adrenergic contractions of blood vessels using selective NaV1.7 inhibitors. Two highly selective NaV1.7 blockers, GNE8493 and PF 05089771, significantly inhibited postganglionic compound action potentials by approximately 70% (P < 0.01), with residual activity being blocked by the NaV1.3 inhibitor, ICA 121431. Electrical field stimulation (EFS) induced rapid contractions in guinea‐pig isolated aorta, pulmonary arteries, and human isolated pulmonary arteries via stimulation of intrinsic nerves, which were inhibited by prazosin or the NaV1 blocker tetrodotoxin. Our results demonstrated that blocking NaV1.7 with GNE8493, PF 05089771, or ST2262 abolished or strongly inhibited sympathetic adrenergic responses in guinea‐pigs and human vascular smooth muscle. These findings support the hypothesis that pharmacologically inhibiting NaV1.7 could potentially reduce sympathetic and parasympathetic function in specific vascular beds and airways.
imageKey points
82% of sympathetic neurons isolated from the stellate ganglion predominantly express NaV1.7 mRNA.
NaV1.7 blockers inhibit action potential conduction in postganglionic sympathetic nerves.
NaV1.7 blockade substantially inhibits sympathetic nerve‐mediated adrenergic contractions in human and guinea‐pig blood vessels.
Pharmacologically blocking NaV1.7 profoundly affects sympathetic and parasympathetic responses in addition to sensory fibres, prompting exploration into the broader physiological consequences of NaV1.7 mutations on autonomic nerve activity.
Seven draft genome sequences of
Streptococcus canis
strains, revealing reduced penicillin-G susceptibility
Authors: Kawara Yuya , Goto Mieko , Maeda Takahiro , Yoshida Haruno , Tsuyuki Yuzo , Takahashi Takashi 
Published: May 2024

ABSTRACT

We report seven draft genome sequences of
Streptococcus canis
strains revealing reduced penicillin-G susceptibility. The genomes measured 2.054–2.385 Mbp, with G+C contents of 38.8%–39.6%. Amino acid substitutions in penicillin-binding proteins were characterized as compared with those of NCTC 12191(T) genome sequence (GenBank accession number
NZ_LR134293.1
).
Lack of abundant core virome in
Culex
mosquitoes from a temperate climate region despite a mosquito species-specific virome
Authors: De Coninck Lander , Soto Alina , Wang Lanjiao , De Wolf Katrien , Smitz Nathalie , Deblauwe Isra , Mbigha Donfack Karelle Celes , Müller Ruth , Delang Leen , Matthijnssens Jelle 
Published: May 2024

ABSTRACT

In arthropod-associated microbial communities, insect-specific viruses (ISVs) are prevalent yet understudied due to limited infectivity outside their natural hosts. However, ISVs might play a crucial role in regulating mosquito populations and influencing arthropod-borne virus transmission. Some studies have indicated a core virome in mosquitoes consisting of mostly ISVs. Employing single mosquito metagenomics, we comprehensively profiled the virome of native and invasive mosquito species in Belgium. This approach allowed for accurate host species determination, prevalence assessment of viruses and
Wolbachia
, and the identification of novel viruses. Contrary to our expectations, no abundant core virome was observed in
Culex
mosquitoes from Belgium. In that regard, we caution against rigidly defining mosquito core viromes and encourage nuanced interpretations of other studies. Nonetheless, our study identified 45 viruses of which 28 were novel, enriching our understanding of the mosquito virome and ISVs. We showed that the mosquito virome in this study is species-specific and less dependent on the location where mosquitoes from the same species reside. In addition, because
Wolbachia
has previously been observed to influence arbovirus transmission, we report the prevalence of
Wolbachia
in Belgian mosquitoes and the detection of several
Wolbachia
mobile genetic elements. The observed prevalence ranged from 83% to 92% in members from the
Culex pipiens
complex.

IMPORTANCE

Culex pipiens
mosquitoes are important vectors for arboviruses like West Nile virus and Usutu virus. Virome studies on individual
Culex pipiens
, and on individual mosquitoes in general, have been lacking. To mitigate this, we sequenced the virome of 190 individual
Culex
and 8 individual
Aedes japonicus
mosquitoes. We report the lack of a core virome in these mosquitoes from Belgium and caution the interpretation of other studies in this light. The discovery of new viruses in this study will aid our comprehension of insect-specific viruses and the mosquito virome in general in relation to mosquito physiology and mosquito population dynamics.
Multi-omics strategy reveals potential role of antimicrobial resistance and virulence factor genes responsible for Simmental diarrheic calves caused by
Escherichia coli
Authors: Shi Zhihai , Lan Yali , Wang Yazhou , Yan Xiangzhou , Ma Xiaoya , Hassan Faiz-ul , Rushdi Hossam E. , Xu Zhaoxue , Wang Wenjia , Deng Tingxian 
Published: May 2024

ABSTRACT

Escherichia coli
(
E. coli
) is reported to be an important pathogen associated with calf diarrhea. Antibiotic resistance genes (ARGs) and virulence factor genes (VFGs) pose a considerable threat to both animal and human health. However, little is known about the characterization of ARGs and VFGs presented in the gut microbiota of diarrheic calves caused by
E. coli
. In this study, we used multi-omics strategy to analyze the ARG and VFG profiles of Simmental calves with diarrhea caused by
E. coli
K99. We found that gut bacterial composition and their microbiome metabolic functions varied greatly in diarrheic calves compared to healthy calves. In total, 175 ARGs were identified, and diarrheal calves showed a significantly higher diversity and abundance of ARGs than healthy calves. Simmental calves with diarrhea showed higher association of VFGs with pili function, curli assembly, and ferrienterobactin transport of
E. coli
. Co-occurrence patterns based on Pearson correlation analysis revealed that
E. coli
had a highly significant (
P
< 0.0001) correlation coefficient (>0.8) with 16 ARGs and 7 VFGs. Metabolomics analysis showed that differentially expressed metabolites in Simmental calves with diarrhea displayed a high correlation with the aforementioned ARGs and VFGs. Phylotype analysis of
E. coli
genomes showed that the predominant phylogroup B1 in diarrheic Simmental calves was associated with 10 ARGs and 3 VFGs. These findings provide an overview of the diversity and abundance of the gut microbiota in diarrheic calves caused by
E. coli
and pave the way for further studies on the mechanisms of antibiotic resistance and virulence in the calves affected with diarrhea.

IMPORTANCE

Simmental is a well-recognized beef cattle breed worldwide. They also suffer significant economic losses due to diarrhea. In this study, fecal metagenomic analysis was applied to characterize the antibiotic resistance gene (ARG) and virulence factor gene (VFG) profiles of diarrheic Simmental calves. We identified key ARGs and VFGs correlated with
Escherichia coli
isolated from Simmental calves. Additionally, metabolomics analysis showed that differentially expressed metabolites in Simmental calves with diarrhea displayed a high correlation with the aforementioned ARGs and VFGs. Our findings provide an insight into the diversity and abundance of the gut microbiota in diarrheic calves caused by
Escherichia coli
and pave the way for further studies on the mechanisms of antibiotic resistance and virulence in the diarrheal calves from cattle hosts.
Proteomics analysis of PK-15 cells infected with porcine parvovirus and the effect of PCBP1 on PPV replication
Authors: Wang Linqing , Song Yue , Xu Menglong , Zhang Chi , Zhang Limeng , Xia Lu , Wei Zhanyong 
Published: May 2024

ABSTRACT

Porcine parvovirus (PPV) is one of the most important pathogens that cause reproductive failure in pigs. However, the pathogenesis of PPV infection remains unclear. Proteomics is a powerful tool to understand the interaction between virus and host cells. In the present study, we analyzed the proteomics of PPV-infected PK-15 cells. A total of 32 and 345 proteins were differentially expressed at the early and replication stages, respectively. Subsequent gene ontology annotation and Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed these differentially expressed proteins were significantly enriched in pathways including toll-like receptor signaling pathway, tumor necrosis factor signaling pathway, and viral carcinogenesis. The expression of poly (rC) binding protein 1 (PCBP1) was observed to decrease after PPV infection. Overexpressed or silenced PCBP1 expression inhibited or promoted PPV infection. Our studies established a foundation for further exploration of the multiplication mechanism of PPV.

IMPORTANCE
Porcine parvovirus (PPV) is a cause of reproductive failure in the swine industry. Our knowledge of PPV remains limited, and there is no effective treatment for PPV infection. Proteomics of PPV-infected PK-15 cells was conducted to identify differentially expressed proteins at 6 hours post-infection (hpi) and 36 hpi. Gene ontology and Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that various pathways participate in PPV infection. Poly (rC) binding protein 1 was confirmed to inhibit PPV replication, which provided potential targets for anti-PPV infection. Our findings improve the understanding of PPV infection and pave the way for future research in this area.
Early Oligocene record of an “iceberg alley” in the Weddell Sea from quartz sand microtextural analysis at ODP Site 696
Authors: Hojnacki Victoria , Passchier Sandra 
Published: May 2024

Abstract
The greenhouse to icehouse transition at the Eocene-Oligocene boundary (34 Ma) marked the appearance of continental-scale glaciation in Antarctica. The material recovered from Ocean Drilling Program Site 696 is the only record spanning this major climatic shift in the Weddell Sea region. Using scanning electron microscopy, quartz microtextures in 13 samples across the Eocene-Oligocene transition were analyzed to understand the degree of glacial modification and the transportation history of the &gt;150 µm material. The quartz grains were visually grouped, characterized, and interpreted by grain outline, relief, and surface microtextures. Glacial textures are present throughout the entire interval (34.4–33.2 Ma), with the proportion of iceberg-rafted grains in each sample decreasing into the early Oligocene (33.6 Ma), accompanied by an increase in the frequency of eolian and sea-ice-rafted grains. Mass accumulation rates reveal that the flux of iceberg-rafted debris increased coincident with the flux of eolian and sea-ice-rafted grains following 33.6 Ma, suggesting a strong coupling between land-ice development and high-latitude atmospheric processes. When compared with other Antarctic climate proxy data sets, the intensification of ice rafting at Site 696 occurred after ice-sheet inception in East Antarctica. The prominent influx of terrigenous material after 33.6 Ma points to strengthened glacial conditions accompanied by major changes in the environment of the Weddell Sea region, supporting the idea of a high-latitude role in climate perturbations, in agreement with interpretations of other global proxies.
Relationship of early participation in physical activities to persistent post-concussive symptoms following acute paediatricpediatric concussion
Authors: Zemek Roger , Grool Anne M , Aglipay Mary , Momoli Franco , Meehan William P , Freedman Stephen B , Yeates Keith Owen , Gravel Jocelyn , Gagnon Isabelle , Boutis Kathy , Meeuwisse Willem , Barrowman Nick , Osmondon Martin H ,   on behalf of the Pediatric Emergency Research Canada (PERC) Concussion Team
Published: May 2024

Trial RegistrationClinicaltrials.gov Indentifier:NCT01873287.ObjectiveTo investigate if early physical activity (7-days post-injury) was associated with lower risk of persistent post-concussive symptoms (PPCS) compared to guideline-recommended conservative rest.DesignProspective, multicenter, cohort study.SettingNine tertiary Canadian Paediatric EDs.Participants2413 children (61% male) aged 5-18 years with acute concussion (<48 hours) with inclusion/exclusion criteria per Predicting Persistent Post-concussive Problems in Paediatrics (5P) study.Assessment of risk factorsParticipation in physical activities, post-concussive symptoms, and concussion modifiers were assessed using standardised questionnaires at baseline, 7- and 28-days post-injury.Outcome measuresPPCS (primary outcome) was defined as ≥3 new or worsening symptoms on Post-Concussion Symptom Inventory at 28-days post-enrolment. The relationship between early physical activity and PPCS was examined using regression, 1:1 propensity-matched, and inverse probability of treatment weighting (IPTW) analyses.ResultsPPCS occurred in 678/2413 (30.1%) participants. Most children (n=1677, 70%) participated in physical activity at one-week of which only n=528 (31%) were symptom-free. Early physical activity was associated with reduced likelihood of PPCS [regression: OR=0.42, (95% CI: 0.35–0.51)]. Early activity remained associated with reduced PPCS risk when matched 1:1 for propensity score [n=1290, OR=0.56, (95% CI: 0.44–0.71)], and using IPTW [n=2235, OR=0.62, (95% CI: 0.51–0.76)]. Sensitivity analyses (replacement of baseline symptoms with one-week symptoms) maintained significant associations [regression: OR=0.42, (95% CI: 0.35–0.51); propensity matching: OR=0.75, (95% CI: 0.60–0.94); and IPTW: OR=0.69, (95% CI: 0.57–0.85)].ConclusionsPhysical activity within 7-days post-injury was associated with lower risk for PPCS in youth recovering from acute concussion. RCTs are urgently needed to confirm these findings and to determine the optimal timing of the initiation of physical activity.Competing interestsNo competing interests to disclose. This study was supported by Canadian Institutes of Health Research (CIHR) Operating Grant MOP:#126197; CIHR-Ontario Neurotrauma Foundation Mild Traumatic Brain Injury Team Grant TM1:#127047; and CIHR planning grant MRP: #119829.None.