bluuebunny/crossref_metadata_2025_split huggingface dataset:
An auction‐based MAC protocol for cognitive radio networks
Authors: Song Hua , Lin Xiao‐La 
Published: October 2011

SUMMARYCognitive radio and dynamic spectrum sharing systems use innovative spectrum management techniques that allow different systems to share the same frequency band to utilize the radio spectrum in an efficient way. In this paper, we propose a novel cognitive media access control protocol for cognitive radio networks under the property‐rights model, in which secondary users are divided into several nonoverlapping groups, and each group uses the proposed auction algorithm to bid for required channels from the auctioneer appointed by primary users. Simulations indicate that our proposed media access control protocol can effectively utilize spectrum resources, achieve high system efficiency, and guarantee the fairness of channel allocation among groups. Copyright © 2011 John Wiley & Sons, Ltd.
Hindered submicron mobility and long‐term storage of presynaptic dense‐core granules revealed by single‐particle tracking
Authors: Scalettar B.A. , Jacobs C. , Fulwiler A. , Prahl L. , Simon A. , Hilken L. , Lochner J.E. 
Published: October 2011

AbstractDense‐core granules (DCGs) are organelles found in neuroendocrine cells and neurons that house, transport, and release a number of important peptides and proteins. In neurons, DCG cargo can include the secreted neuromodulatory proteins tissue plasminogen activator (tPA) and/or brain‐derived neurotrophic factor (BDNF), which play a key role in modulating synaptic efficacy in the hippocampus. This function has spurred interest in DCGs that localize to synaptic contacts between hippocampal neurons, and several studies recently have established that DCGs localize to, and undergo regulated exocytosis from, postsynaptic sites. To complement this work, we have studied presynaptically localized DCGs in hippocampal neurons, which are much more poorly understood than their postsynaptic analogs. Moreover, to enhance relevance, we visualized DCGs via fluorescence labeling of exogenous and endogenous tPA and BDNF. Using single‐particle tracking, we determined trajectories of more than 150 presynaptically localized DCGs. These trajectories reveal that mobility of DCGs in presynaptic boutons is highly hindered and that storage is long‐lived. We also computed mean‐squared displacement curves, which can be used to elucidate mechanisms of transport. Over shorter time windows, most curves are linear, demonstrating that DCG transport in boutons is driven predominantly by diffusion. The remaining curves plateau with time, consistent with motion constrained by a submicron‐sized corral. These results have relevance to recent models of presynaptic organization and to recent hypotheses about DCG cargo function. The results also provide estimates for transit times to the presynaptic plasma membrane that are consistent with measured times for onset of neurotrophin release from synaptically localized DCGs. © 2011 Wiley Periodicals, Inc. Develop Neurobiol, 2012
Primary cilia and Gli3 activity regulate cerebral cortical size
Authors: Wilson Sandra L. , Wilson John P. , Wang Chengbing , Wang Baolin , McConnell Susan K. 
Published: October 2011

AbstractDuring neural development patterning, neurogenesis, and overall growth are highly regulated and coordinated between different brain regions. Here, we show that primary cilia and the regulation of Gli activity are necessary for the normal expansion of the cerebral cortex. We show that loss of Kif3a, an important functional component of primary cilia, leads to the degeneration of primary cilia, marked overgrowth of the cortex, and altered cell cycle kinetics within cortical progenitors. The G1 phase of the cell cycle is shortened through a mechanism likely involving reduced Gli3 activity and a resulting increase in expression of cyclin D1 and Fgf15. The defects in Gli3 activity alone are sufficient to accelerate cell cycle kinetics and cause the molecular changes seen in brains that lack cilia. Finally, we show that levels of full‐length and repressor Gli3 proteins are tightly regulated during normal development and correlate with changes in expression of two known Shh‐target genes, CyclinD1 and Fgf15, and with the normal lengthening of the cell cycle during corticogenesis. These data suggest that Gli3 activity is regulated through the primary cilium to control cell cycle length in the cortex and thus determine cortical size. © 2011 Wiley Periodicals, Inc. Develop Neurobiol, 2012
Generation of Rab‐based transgenic lines for in vivo studies of endosome biology in zebrafish
Authors: Clark Brian S. , Winter Mark , Cohen Andrew R. , Link Brian A. 
Published: October 2011

AbstractThe Rab family of small GTPases function as molecular switches regulating membrane and protein trafficking. Individual Rab isoforms define and are required for specific endosomal compartments. To facilitate in vivo investigation of specific Rab proteins, and endosome biology in general, we have generated transgenic zebrafish lines to mark and manipulate Rab proteins. We also developed software to track and quantify endosome dynamics within time‐lapse movies. The established transgenic lines ubiquitously express EGFP fusions of Rab5c (early endosomes), Rab11a (recycling endosomes), and Rab7 (late endosomes) to study localization and dynamics during development. Additionally, we generated UAS‐based transgenic lines expressing constitutive active (CA) and dominant‐negative (DN) versions for each of these Rab proteins. Predicted localization and functional consequences for each line were verified through a variety of assays, including lipophilic dye uptake and Crumbs2a localization. In summary, we have established a toolset for in vivo analyses of endosome dynamics and functions. Developmental Dynamics 240:2452–2465, 2011. © 2011 Wiley Periodicals, Inc.
Deficient CCR7 signaling promotes TH2 polarization and B‐cell activation in vivo
Authors: Moschovakis G. L. , Bubke Anja , Dittrich‐Breiholz Oliver , Braun Asolina , Prinz Immo , Kremmer Elisabeth , Förster Reinhold 
Published: October 2011

AbstractThe chemokine receptor CCR7 has a central role in regulating homing and positioning of T cells and DCs to lymph nodes (LNs) and participates in T‐cell development and activation. In this study, we addressed the role of CCR7 signaling in TH2 polarization and B‐cell activation. We provide evidence that the lack of CCR7 drives the capacity of naïve CD4+T cells to polarize toward TH2 cells. This propensity contributes to a lymph node environment in CCR7‐deficent mice characterized by increased expression of IL‐4 and increased frequency of TH2 cells. We show that elevated IL‐4 levels lead to B‐cell activation characterized by up‐regulated expression of MHC class II, CD23 and CD86. Activated B cells are in turn highly efficient in presenting antigen to CD4+T cells and thus potentially contribute to the TH2 microenvironment. Taken together, our results support the idea of a CCR7‐dependent patterning of TH2 responses, with absent CCR7 signaling favoring TH2 polarization, dislocation of T helper cells into the B‐cell follicles and, as a consequence, B‐cell activation.
DOCK8 is essential for T‐cell survival and the maintenance of CD8+ T‐cell memory
Authors: Lambe Teresa , Crawford Greg , Johnson Andy L. , Crockford Tanya L. , Bouriez‐Jones Tiphaine , Smyth Aisling M. , Pham Trung H. M. , Zhang Qian , Freeman Alexandra F. , Cyster Jason G. , Su Helen C. , Cornall Richard J. 
Published: October 2011

AbstractDeficiency in the guanine nucleotide exchange factor dedicator of cytokinesis 8 (DOCK8) causes a human immunodeficiency syndrome associated with recurrent sinopulmonary and viral infections. We have recently identified a DOCK8‐deficient mouse strain, carrying an ethylnitrosourea‐induced splice‐site mutation that shows a failure to mature a humoral immune response due to the loss of germinal centre B cells. In this study, we turned to T‐cell immunity to investigate further the human immunodeficiency syndrome and its association with decreased peripheral CD4+ and CD8+ T cells. Characterisation of the DOCK8‐deficient mouse revealed T‐cell lymphopenia, with increased T‐cell turnover and decreased survival. Egress of mature CD4+ thymocytes was reduced with increased migration of these cells to the chemokine CXCL12. However, despite the two‐fold reduction in peripheral naïve T cells, the DOCK8‐deficient mice generated a normal primary CD8+ immune response and were able to survive acute influenza virus infection. The limiting effect of DOCK8 was in the normal survival of CD8+ memory T cells after infection. These findings help to explain why DOCK8‐deficient patients are susceptible to recurrent infections and provide new insights into how T‐cell memory is sustained.
Influence of Zataria multiflora Boiss. essential oil on oxidative stability of sunflower oil
Authors: Hashemi Mohammad Bagher , Niakousari Mehrdad , Saharkhiz Mohammad Jamal , Eskandari Mohammad Hadi 
Published: October 2011

AbstractIn this study, the influence of the application of 0.025%, 0.05% and 0.075% of Zataria multiflora Boiss. essential oil (EO) on oxidative stability of sunflower oil was examined and the EO was compared to butylated hydroxyanisole (BHA) and butylated hydroxytoluene (BHT) during storage at 37°C and 47°C. The main components of EO were identified as carvacrol (45.6%), p‐cymene (18.1%) and thymol (16.3%). Peroxide value (PV), anisidine value (AnV) and thiobarbituric acid (TBA) value measurement in sunflower oil showed that all concentrations of EO had a lower antioxidant effect in comparison to BHA and BHT. Samples supplemented with EO concentration of 0.075% were the most stable during storage at both temperatures (p<0.05). Furthermore, Totox value, antioxidant activity (AA), stabilization factor (F) and antioxidant power (AOP) determination confirmed efficacy of this EO as antioxidant in sunflower oil. EO also was able to reduce the stable 2,2‐diphenyl‐1‐picrylhydrazyl free radical (DPPH.) with a 50% inhibition concentration (IC50) of 34.3 ± 0.8 µg/mL. The results indicate that EO could be used as a natural antioxidant in oils for food uses.
Self‐judgment and reputation monitoring as a function of the fundamental dimensions, temporal perspective, and culture
Authors: Ybarra Oscar , Park Hyekyung , Stanik Christine , Lee David Seungjae 
Published: October 2011

AbstractSocial acceptance and the development of one's competencies and status are fundamental aspects of the human experience, but the former (communion) should take precedence over the latter (agency) in self‐judgment. Study 1 results indicated that (i) people across two cultures judged themselves as possessing higher communion than agency characteristics; (ii) communion self‐judgments were more consistent across temporal perspective; and (iii) level of self‐enhancement across cultures was similar for communion but different for agency. In Study 2, people across culture reported being more troubled and demonstrated a greater desire to repair their reputation when they imagined others perceived them as lacking in communion compared with agency. These findings support the idea that social life pressures people to view themselves as possessing communion traits and to ensure that others have this perception as well. Copyright © 2011 John Wiley & Sons, Ltd.
Perceptions of non‐target confronters in response to racist and heterosexist remarks
Authors: Dickter Cheryl L. , Kittel Julie A. , Gyurovski Ivo I. 
Published: October 2011

AbstractResearch has established that targets who express disagreement with prejudicial comments directed toward their social group may be viewed negatively by those they confront or by members of social outgroups. Less research has examined how non‐target individuals who confront prejudicial remarks are perceived. The current studies were designed to examine how non‐targets who confronted racist (Study 1) and heterosexist (Study 2) comments would be perceived as a function of the level of offensiveness of the comment and the confrontation style used. The studies also examined whether confronting behavior would affect perceptions of the individual who made the prejudicial comment. Undergraduate participants read vignettes depicting a situation with a high or low offensive prejudicial comment in which a non‐target individual confronted assertively, unassertively, or not at all. Participants provided judgments of both individuals. Results indicated that non‐targets who confronted highly prejudicial comments either assertively or unassertively were liked and respected more than those who failed to confront. Additionally, commenters who were assertively confronted were respected less than commenters who were not. These findings suggest that non‐targets may be especially effective in confronting prejudicial comments, as they do not suffer the same negative consequences as targets who confront. Copyright © 2011 John Wiley & Sons, Ltd.
Surface modification of poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) copolymer films for promoting interaction with bladder urothelial cells
Authors: García‐García José M. , López Laura , París Rodrigo , Núñez‐López María Teresa , Quijada‐Garrido Isabel , de la Peña Zarzuelo Enrique , Garrido Leoncio 
Published: October 2011

AbstractOften bladder dysfunction and diseases lead to therapeutic interventions that require partial or complete replacement of damaged tissue. For this reason, the development of biomaterials to repair the bladder by promoting the adhesion and growth of urothelial cells is of interest. With this aim, a modified copolyester of biocompatible and biodegradable poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) [P(HB‐co‐HV)] was used as scaffold for porcine urothelial cell culture. In addition to good biocompatibility, the surface of P(HB‐co‐HV) substrates was modified to provide both, higher hydrophilicity and a better interaction with urothelial cells. Chemical treatments with ethylenediamine (ED) and sodium hydroxide (NaOH) led to substrate surfaces with decreasing hydrophobicity and provided functional groups that enable the grafting of bioactive molecules, such as a laminin derived YIGSR sequence. Physico‐chemical properties of modified substrates were studied and compared with those of the pristine P(HB‐co‐HV). Urothelial cell morphology on treated substrates was studied. The results showed that focal attachment and cell‐related properties were improved for peptide grafted polymer compared with both, the unmodified and functionalized copolyester. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.
Mediation of the migration of endothelial cells and fibroblasts on polyurethane nanocomposites by the activation of integrin‐focal adhesion kinase signaling
Authors: Hung Huey‐Shan , Chu Mei‐Yun , Lin Chien‐Hsun , Wu Chia‐Ching , Hsu Shan‐hui 
Published: October 2011

AbstractModel surfaces of polyurethane‐gold nanocomposites (PU‐Au) were used to examine cell behavior on nanophase‐segregated materials. Previously we showed that endothelial cell (EC) migration on these materials was modulated by the PI3K/Akt/eNOS pathway. The present study, investigated the expressions of alpha5/beta3 (α5β3) integrin, focal adhesion kinase (FAK), and other downstream signal molecules such as the Rho family and matrix metalloproteinases 2 (MMP‐2) induced by the materials in two different cells, that is bovine arterial endothelial cells (BAEC) and human skin fibroblasts (HSF). Both cells proliferated better on the more phase‐separated PU‐Au 43.5 ppm than on the less phase‐separated controls (PU and PU‐Au 174 ppm). On PU‐Au 43.5 ppm, BAEC compared to HSF had denser actin fibers and were more extended. BAEC became rounded with Y‐27632 treatment and shrunk with LY294002 treatment. Treatment by inhibitors only caused slight changes in HSF. The migration distance of BAEC on PU‐Au 43.5 ppm was greater than that of HSF, and was significantly reduced by LY294002 or Y‐27632 but not SU‐1498. The expressions of p‐FAK, p‐RhoA, p‐Rac/Cdc42, MMP2, and α5β3 integrin induced by PU‐Au 43.5 ppm were more pronounced in BAEC versus HSF. Further enhancement in MMP2 and α5β3 integrin expressions by FAK‐GFP transfection was more remarkable for cells on PU‐Au 43.5 ppm. Our findings suggested that the integrin α5β3/FAK pathway may be induced by nanophase‐separated materials in both ECs and fibroblasts to promote their proliferation/migration, while the crosstalk between the PI3K/Akt/eNOS pathway and FAK/Rho‐GTPase activation may account for the greater effect in ECs than in fibroblasts. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.
Bone mineral density loss in relation to the final menstrual period in a multiethnic cohort: Results from the Study of Women's Health Across the Nation (SWAN)
Authors: Greendale Gail A , Sowers MaryFran , Han Weijuan , Huang Mei-Hua , Finkelstein Joel S , Crandall Carolyn J , Lee Jennifer S , Karlamangla Arun S 
Published: October 2011

Abstract
The objective of this study was to describe the time of onset and offset of bone mineral density (BMD) loss relative to the date of the final menstrual period (FMP); the rate and amount of BMD decline during the 5 years before and the 5 years after the FMP; and the independent associations between age at FMP, body mass index (BMI), and race/ethnicity with rates of BMD loss during this time interval. The sample included 242 African American, 384 white, 117 Chinese, and 119 Japanese women, pre- or early perimenopausal at baseline, who had experienced their FMP and for whom an FMP date could be determined. Loess-smoothed curves showed that BMD loss began 1 year before the FMP and decelerated (but did not cease) 2 years after the FMP, at both the lumbar spine (LS) and femoral neck (FN) sites. Piecewise, linear, mixed-effects regression models demonstrated that during the 10-year observation period, at each bone site, the rates and cumulative amounts of bone loss were greatest from 1 year before through 2 years after the FMP, termed the transmenopause. Postmenopausal loss rates, those occurring between 2 and 5 years after the FMP, were less than those observed during transmenopause. Cumulative, 10-year LS BMD loss was 10.6%; 7.38% was lost during the transmenopause. Cumulative FN loss was 9.1%; 5.8% was lost during the transmenopause. Greater BMI and African American heritage were related to slower loss rates, whereas the opposite was true of Japanese and Chinese ancestry. © 2012 American Society for Bone and Mineral Research
Retracted: Pro‐inflammatory cytokines induce odontogenic differentiation of dental pulp‐derived stem cells
Authors: Yang Xuechao , Zhang Siyuan , Pang Xin , Fan Mingwen 
Published: October 2011

AbstractRetraction: The following article from Journal of Cellular Biochemistry, “Pro‐inflammatory cytokines induce odontogenic differentiation of dental pulp‐derived stem cells,” by Xuechao Yang, Siyuan Zhang, Xin Pang, and Mingwen Fan, published online on January 5, 2012 in Wiley Online Library (http://onlinelibrary.wiley.com), has been retracted by agreement between the authors, the journal Editor in Chief, Gary S. Stein and Wiley Periodicals, Inc. The retraction has been agreed due to overlap with the article published in Journal of Tissue Engineering and Regenerative Medicine, “Retracted: Effects of pro‐inflammatory cytokines on mineralization potential of rat dental pulp stem cells,” by Xuechao Yang, X. Frank Walboomers, Zhuan Bian, John A. Jansen, and Mingwen Fan published online on July 11, 2011.
Dynamic alterations in integrin α4 expression by hypoxia are involved in trophoblast invasion during early implantation
Authors: Na Kyu‐Hwan , Lee Hyun‐Jung , Choi Jong‐Ho , Eun Jung‐Woo , Nam Suk‐Woo , Yoon Tae‐Ki , Kim Gi‐Jin 
Published: October 2011

AbstractImplantation of the blastocyst into the maternal endometrium is mediated by a population of well‐differentiated primary cells of the placenta known as trophoblasts, which grow in an invasive and destructive fashion similar to tumor cells. Interactions between the endometrium and trophoblasts are regulated by a coordinated interplay of extracellular matrix (ECM) proteins secreted by the invading extravillous trophoblasts. Integrins act as adhesion receptors and mediate both cell–ECM and cell–cell interactions. However, the correlation between integrin expression and trophoblast invasion under hypoxia is unclear. Here, we analyzed the expression of integrins in HTR‐8/SVneo trophoblast cells exposed to hypoxic conditions in order to demonstrate an association between invasion activity and integrin expression in trophoblasts. Trophoblasts were examined by microarray analysis, RT‐PCR, western blotting, and zymography after 1% hypoxic treatment, and cell invasion was estimated. The dynamic expression of integrins and human matrix metalloproteinases (MMPs) was observed under hypoxic conditions. The invasiveness of trophoblasts cultured under 1% hypoxic conditions was significantly greater than that of trophoblasts cultured under normoxic conditions through alterations in MMP‐2 and ‐9 (P < 0.05). Notably, integrin α4 expression during early hypoxia was negatively regulated by hypoxia‐inducible factor‐1alpha (HIF‐1alpha) expression in trophoblasts. The downregulation of integrin α4 expression by siRNA treatment controlled trophoblast invasion activity (P < 0.05). Taken together, we suggest that dynamic changes in integrins, including those in integrin α4 expression by hypoxia, play a regulatory role in trophoblast invasion. These findings expand our understanding of the potential roles of integrin α4 in implantation. J. Cell. Biochem. 113: 685–694, 2012. © 2011 Wiley Periodicals, Inc.
Role of gap junctions in chronic pain
Authors: Wu Ann , Green Colin R. , Rupenthal Ilva D. , Moalem‐Taylor Gila 
Published: October 2011

AbstractGap junctions are specialized transmembrane channels that allow rapid electrical signalling and direct intercellular communication for maintenance and coordination of normal cellular activities and homeostasis. Although gap junction channels in the nervous system mediate intercellular coupling between glial cells and between neurons, they also contribute to the spread of secondary damage and inflammation under pathological conditions. There is now evidence of the involvement of gap junctions in chronic pain caused by nervous system damage or tissue inflammation. In this Mini‐Review, we highlight recent studies demonstrating the dynamic plasticity of gap junctions in response to nervous system injury and the effects of gap junction blockade on neuronal survival and modulation of pain in animal models of neuropathic and inflammatory pain. The involvement of dorsal root ganglia and spinal cord gap junctions in mediating chronic pain and the potential for targeting connexins as a novel modality for the treatment of intractable pain syndromes arising from nervous system injury and disorders are discussed. © 2011 Wiley Periodicals, Inc.