bluuebunny/crossref_metadata_2025_split huggingface dataset:
Arachidonate 5 lipoxygenase expression in papillary thyroid carcinoma promotes invasion via MMP‐9 induction
Authors: Kummer Nicolas T. , Nowicki Theodore S. , Azzi Jean P. , Reyes Ismael , Iacob Codrin , Xie Suqing , Swati Ismatun , Darzynkiewicz Zbigniew , Gotlinger Katherine H. , Suslina Nina , Schantz Stimson , Tiwari Raj K. , Geliebter Jan 
Published: January 2012

AbstractArachidonate 5‐lipoxygenase (ALOX5) expression and activity has been implicated in tumor pathogenesis, yet its role in papillary thyroid carcinoma (PTC) has not been characterized. ALOX5 protein and mRNA were upregulated in PTC compared to matched, normal thyroid tissue, and ALOX5 expression correlated with invasive tumor histopathology. Evidence suggests that PTC invasion is mediated through the induction of matrix metalloproteinases (MMPs) that can degrade and remodel the extracellular matrix (ECM). A correlation between MMP‐9 and ALOX5 protein expression was established by immunohistochemical analysis of PTC and normal thyroid tissues using a tissue array. Transfection of ALOX5 into a PTC cell line (BCPAP) increased MMP‐9 secretion and cell invasion across an ECM barrier. The ALOX5 product, 5(S)‐hydroxyeicosatetraenoic acid also increased MMP‐9 protein expression by BCPAP in a dose‐dependent manner. Inhibitors of MMP‐9 and ALOX5 reversed ALOX5‐enhanced invasion. Here we describe a new role for ALOX5 as a mediator of invasion via MMP‐9 induction; this ALOX5/MMP9 pathway represents a new avenue in the search for functional biomarkers and/or potential therapeutic targets for aggressive PTC. J. Cell. Biochem. 113: 1998–2008, 2012. © 2012 Wiley Periodicals, Inc.
Osteoclastogenesis is negatively regulated by D‐serine produced by osteoblasts
Authors: Takarada Takeshi , Takarada‐Iemata Mika , Takahata Yoshifumi , Yamada Daisuke , Yamamoto Tomomi , Nakamura Yukari , Hinoi Eiichi , Yoneda Yukio 
Published: January 2012

AbstractWe have shown the functional expression by chondrocytes of serine racemase (SR) which is responsible for the synthesis of D‐serine (Ser) from L‐Ser in cartilage. In this study, we evaluated the possible functional expression of SR by bone‐forming osteoblasts and bone‐resorbing osteoclasts. Expression of SR mRNA was seen in osteoblasts localized at the cancellous bone surface in neonatal rat tibial sections and in cultured rat calvarial osteoblasts endowed to release D‐Ser into extracellular medium, but not in cultured osteoclasts differentiated from murine bone marrow progenitor cells. Sustained exposure to D‐Ser failed to significantly affect alkaline phosphatase activity and Ca2+ accumulation in cultured osteoblasts, but significantly inhibited differentiation and maturation in a concentration‐dependent manner at a concentration range of 0.1–1 mM without affecting cellular survival in cultured osteoclasts. By contrast, L‐Ser promoted osteoclastic differentiation in a manner sensitive to the inhibition by D‐Ser. Matured osteoclasts expressed mRNA for the amino acid transporter B0,+ (ATB0,+) and the system alanine, serine, and cysteine amino acid transporter‐2 (ASCT2), which are individually capable of similarly incorporating extracellular L‐ and D‐Ser. Knockdown of these transporters by siRNA prevented both the promotion by L‐Ser and the inhibition by D‐Ser of osteoclastic differentiation in pre‐osteoclastic RAW264.7 cells. These results suggest that D‐Ser may play a pivotal role in osteoclastogenesis through a mechanism related to the incorporation mediated by both ATB0,+ and ASCT2 of serine enantiomers in osteoclasts after the synthesis and subsequent release from adjacent osteoblasts. J. Cell. Physiol. 227: 3477–3487, 2012. © 2012 Wiley Periodicals, Inc.
Biological effects of the plant‐derived polyphenol resveratrol in human articular cartilage and chondrosarcoma cells
Authors: Im Hee‐Jeong , Li Xin , Chen Di , Yan Dongyao , Kim Jaesung , Ellman Michael B. , Stein Gary S. , Cole Brian , KC Ranjan , Cs‐Szabo Gabriella , van Wijnen Andre J. 
Published: January 2012

AbstractThe natural phytoestrogen resveratrol (RSV) may have therapeutic potential for arthritic conditions. RSV is chondroprotective for articular cartilage in rabbit models for arthritis, but its biological effects on human articular cartilage and chondrosarcoma cells are unknown. Effects of RSV on human articular cartilage homeostasis were studied by assessing production of matrix‐degrading enzymes (MMP‐13, ADAMTS4, and ADAMTS5), as well as proteoglycan production and synthesis. The counteractions of RSV against catabolic factors (e.g., FGF‐2 or IL‐1β) were examined by in vitro and ex vivo using monolayer, three‐dimensional alginate beads and cartilage explants cultures, respectively. RSV improves cell viability of articular chondrocytes and effectively antagonizes cartilage‐degrading protease production that was initiated by catabolic and/or anti‐anabolic cytokines in human articular chondrocytes. RSV significantly also enhances BMP7‐promoted proteoglycan synthesis as assessed by 35S‐sulfate incorporation. Protein–DNA interaction arrays suggest that RSV inhibits the activation of transcription factors involved in inflammation and cartilage catabolic signaling pathways, including direct downstream regulators of MAPK (e.g., AP‐1, PEA3) and NFκB. RSV selectively compromises survival of human chondrosarcoma cells, but not primary articular chondrocytes, revealing cell‐specific activity of RSV on non‐tumorigenic versus tumor‐derived cells. We propose that RSV exerts its chondroprotective functions, in part, by deactivating p53‐induced apoptosis in human primary chondrocytes, but not human chondrosarcoma. Our findings suggest that RSV has potential as a unique biologic treatment for both prevention and treatment of cartilage degenerative diseases. J. Cell. Physiol. 227: 3488–3497, 2012. © 2012 Wiley Periodicals, Inc.
The morphological findings of malleus and incus in a case of Marfan's syndrome
Authors: Evcimik Muhammed Fatih , Ozkurt Fazil Emre , Karavus Ahmet , Sapci Tarik 
Published: January 2012

AbstractIn Marfan's syndrome, which is a connective tissue disorder of hereditary origin, collagenous tissue development and bone synthesis are generally altered in addition to the occurrence of many systemic deformities. External ear, stapes, and vestibular aqueduct pathologies are reported as some of the otological deformities. However, the malleus and incus pathologies of the ear are not reported in any study. In the morphometric measurements carried out, the distance between the malleus head and manubrium was found to be 9.8 mm. When similar measurements were carried out for the incus, the distance between the upper part of the incus body and tip of the long process was found to be 6.9 mm. All of these measurements were detected to be greater than in normal people. It can be concluded from this study that in Marfan's syndrome, in addition to the musculoskeletal alterations particularly in bone synthesis, the ossicular chain in the middle ear is also affected.
Radiological pitfalls with laryngopharyngeal injectables
Authors: Shuaib Stefan W. , Potter Andrew A. , Ginsberg Lawrence E. , Kupferman Michael E. 
Published: January 2012

AbstractThe aim of this report was to describe the computed tomography features of injected carboxymethylcellulose and how it can be mistaken for tumor recurrence. Carboxymethylcellulose is a low‐density injectable indicated for short‐term vocal fold augmentation. When used in patients with head and neck cancer, differentiating this agent from a neoplastic recurrence can be difficult on imaging. The imaging findings of five patients who received carboxymethylcellulose augmentation are presented to illustrate a potential pitfall of image interpretation. When injectable carboxymethylcellulose is used in cancer patients, knowledge of the injection sites, material used, and procedural history can avoid misinterpretation of monitory imaging.
Genetic characterization of vocal fold lesions: Leukoplakia and Carcinoma
Authors: Bartlett Rebecca S. , Heckman W. Wesley , Isenberg Jason , Thibeault Susan L. , Dailey Seth H. 
Published: January 2012

AbstractObjectives/Hypothesis:Malignant transformation of laryngeal keratosis has been reported in a substantial subset of patients, yet reliable criteria for predicting patients most at risk have yet to be determined. Current methods for determining dysplasia ratings are susceptible to errors in biopsy sampling and interpretation. An understanding of the genetic underpinnings of the progression of vocal fold tumorigenesis may contribute to the creation of reliable and predictive diagnostic criteria. We hypothesized that genetic expression markers distinguish patients with keratotic noncancerous vocal fold lesions from invasive carcinoma.Study Design:Observational cross‐sectional study.Methods:Real‐time polymerase chain reaction (RT‐PCR) was used to compare expression of 84 cancer pathway genes of patients following histologic diagnosis of nondysplastic keratotic epithelium (ND) (n = 7), dysplastic keratotic epithelium (DYS) (n = 3), and invasive carcinoma (CA) (n = 7). All patients had a clinical diagnosis of leukoplakia, and biopsies were obtained from true vocal fold tissue.Results:Four genes (IGF‐1, EPDR1, MMP‐2, S100A4) were significantly upregulated in DYS over the ND group. Seven genes were significantly upregulated in CA over the DYS group, and 31 genes were significantly upregulated in CA over the ND group (P < .02). The expression of matrix metalloproteinases (MMP‐1, MMP‐2, MMP‐9) was found to statistically differentiate the groups (P < .02) and suggested disease progression associated with extracellular matrix degradation and angiogenesis promotion.Conclusions:With these preliminary array data, we demonstrate the feasibility of using RT‐PCR to identify distinct genetic expression between diagnostic groups. Characterization of genetic changes marking the progression of vocal fold tumorigenesis may lead to robust diagnostic criteria in the future.
Protecting the oral mucosa in patients with oral tongue squamous cell carcinoma treated postoperatively with intensity‐modulated radiotherapy: A randomized study
Authors: Wang Zhong‐He , Zhang Shi‐Zhou , Zhang Zhi‐Yuan , Zhang Chen‐Ping , Hu Hai‐Sheng , Tu Wen‐Yong , Kirwan Jessica , Mendenhall William M. 
Published: January 2012

AbstractObjectives/Hypothesis:Is the severity of acute oral mucositis in patients who receive postoperative intensity‐modulated radiotherapy (PO‐IMRT) for oral tongue squamous cell carcinoma (SCC) reduced by sparing the oral mucosa outside of the planning target volume (PTV)?Study Design:Prospective, randomized trial.Methods:Forty‐eight patients with oral tongue SCC who received PO‐IMRT at our institution were randomized to two groups: the oral‐sparing (OR‐SP) group and oral‐unsparing (OR‐USP) group. For the OR‐SP group (n = 24), the oral mucosa outside of the PTV was spared. Furthermore, the mucosa including the bilateral cheeks, upper lip, and lower lip was defined as the united site and given <32 Gy. For the OR‐USP group (n = 24), none of the oral mucosa was protected. The severity of clinical acute mucositis in each patient was assessed weekly during PO‐IMRT until completely healed. Oral mucositis was graded according to the National Cancer Institute Common Terminology Criteria for Adverse Events version 3.0. Dosimetry and therapeutic measures related to acute mucositis between the two groups were compared.Results:During PO‐IMRT, no patient experienced grade 4+ acute mucositis in any oral site. Compared to the OR‐USP group, there was less grade 2 and 3 mucositis in the united site of the OR‐SP group (0% and 25% vs. 45.8% and 54.2%, respectively; P = .000). Also, the mean dose to the united site was significantly lower with OR‐SP compared to OR‐USP (41.8 ± 7.4 Gy vs. 58.8 ± 2.2 Gy; P = .000). The OR‐SP group was associated with significant reductions in the use of analgesics (P = .043) and intravenous antibiotics (P = .039). No recurrences were detected in the vicinity of the spared oral mucosa (the united site) during a median follow‐up time of 30 months.Conclusions:OR‐SP PO‐IMRT for patients with oral tongue SCC resulted in a significant decrease in the severity of acute mucositis and improved quality of life. The sparing of the oral mucosa outside of the PTV is safe and does not compromise oncologic outcomes.
Relaxation‐corrected bootstrap algebraic multigrid (rBAMG)
Authors: Brezina M. , Ketelsen C. , Manteuffel T. , McCormick S. , Park M. , Ruge J. 
Published: January 2012

SUMMARYBootstrap algebraic multigrid (BAMG) is a multigrid‐based solver for matrix equations of the form Ax = b. Its aim is to automatically determine the interpolation weights used in algebraic multigrid by locally fitting a set of test vectors that have been relaxed as solutions to the corresponding homogeneous equation, Ax = 0. This paper studies an improved form of BAMG, called relaxation‐corrected bootstrap algebraic multigrid (rBAMG), that involves adding scaled residuals of the test vectors to the least‐squares equations.The basic rBAMG scheme was introduced in an earlier paper [1] and analyzed on a simple model problem. The purpose of the current paper is to further develop this algorithm by incorporating several new critical components and to systematically study its performance on an interesting model problem from quantum chromodynamics. Whereas the earlier paper introduced a new least‐squares principle involving the residuals of the test vectors, a simple extrapolation scheme is developed here to accurately estimate the convergence factors of the evolving algebraic multigrid solver. Such a capability is essential to the effective development of a fast solver, and the approach introduced here is shown numerically to be much more effective than the conventional approach of just observing successive error reduction factors. Another component of the setup process developed here is an adaptive cycling process. This component assesses the effectiveness of the V‐cycle constructed in the initial rBAMG phase by applying it to the homogeneous equation. When poor convergence is observed, the set of test vectors is enhanced with the resulting error, enabling the subsequent least‐squares fit of interpolation to produce an improved V‐cycle. A related component is the scaling and recombination Ritz process that targets the so‐called weak approximation property in an attempt to reveal the important elements of these evolving error and test vector spaces.The aim of the numerical study documented here is to provide insight into the various design choices that arise in the development of an rBAMG algorithm. With this in mind, the results for quantum chromodynamics focus on the behavior of rBAMG in terms of the number of initial test vectors used, the number of relaxation sweeps applied to them, and the size of the target matrices. Copyright © 2012 John Wiley & Sons, Ltd.
Seven years of experience with high‐intensity focused ultrasound for prostate cancer: Advantages and limitations
Authors: Sung Hyun Hwan , Jeong Byong Chang , Seo Seong Il , Jeon Seong Soo , Choi Han‐Yong , Lee Hyun Moo 
Published: January 2012

AbstractBACKGROUNDThe aim of this study was to evaluate oncologic outcomes and adverse events for patients with prostate cancer after treatment by high‐intensity focused ultrasound (HIFU).METHODSBetween February 2004 and August 2010, patients with prostate cancer who underwent HIFU treatment were reviewed about oncologic outcomes and complications. Biochemical recurrence (BCR) was defined as prostate‐specific antigen (PSA) nadir plus 1.2 ng/ml. Kaplan–Meier analysis was performed to evaluate BCR‐ and disease progression‐free survival according to risk stratification. Predictors for BCR and disease progression were identified using the Cox‐proportional hazard method.RESULTSThe overall BCR rate was 59.5%, and median time to BCR was 13.8 months. The 5‐year BCR‐free survival rates of the low‐, intermediate‐, and high‐risk groups were 66.3, 40.2, and 21.0% (P = 0.001), respectively, and the 5‐year disease progression‐free survival rates were 73.5, 46.0, and 29.2%, respectively (P = 0.008). Multivariate analysis showed that risk stratification, PSA nadir, and time to PSA nadir were significant predictors of BCR and disease progression. In the first 3 months post‐op, 11 patients (8.7%) had complications. There were no patients who required blood transfusions or who had wound problems, stroke, deep vein thrombosis, or bowel dysfunction.CONCLUSIONSHIFU treatment does not provide effective oncologic outcomes even in low risk patients with prostate cancer as well as in the intermediate or high risk groups. Therefore, patients selected to undergo HIFU treatment for prostate cancer must be very carefully chosen. On the other hand, HIFU treatment for prostate cancer had a very low rate of complications. Prostate 72:1399–1406, 2012. © 2012 Wiley Periodicals, Inc.
The binding affinities of proteins interacting with the PDZ domain of PICK1
Authors: Bolia Ashini , Gerek Z. Nevin , Keskin Ozlem , Banu Ozkan Sefika , Dev Kumlesh K. 
Published: January 2012

AbstractProtein interacting with C kinase (PICK1) is well conserved throughout evolution and plays a critical role in synaptic plasticity by regulating the trafficking and posttranslational modification of its interacting proteins. PICK1 contains a single PSD95/DlgA/Zo‐1 (PDZ) protein–protein interaction domain, which is promiscuous and shown to interact with over 60 proteins, most of which play roles in neuronal function. Several reports have suggested the role of PICK1 in disorders such as epilepsy, pain, brain trauma and stroke, drug abuse and dependence, schizophrenia and psychosis. Importantly, lead compounds that block PICK1 interactions are also now becoming available. Here, a new modeling approach was developed to investigate binding affinities of PDZ interactions. Using these methods, the binding affinities of all major PICK1 interacting proteins are reported and the effects of PICK1 mutations on these interactions are described. These modeling methods have important implications in defining the binding properties of proteins interacting with PICK1 as well as the general structural requirements of PDZ interactions. The study also provides modeling methods to support in the drug design of ligands for PDZ domains, which may further aid in development of the family of PDZ domains as a drug target. Proteins 2012;. © 2012 Wiley Periodicals, Inc.
Navigating the language demands of an inquiry‐based science performance assessment: Classroom challenges and opportunities for English learners
Authors: Lyon Edward G. , Bunch George C. , Shaw Jerome M. 
Published: January 2012

AbstractScience performance assessments (SPAs) are designed to elicit a wider range of scientific knowledge and abilities than ordinarily measured by more traditional paper‐and‐pencil tests. To engage in SPAs and thus demonstrate abilities such as scientific inquiry, students must interact with various participants and communicate in a variety of ways. Using an analytical framework that draws on functional and interactional views of language, we analyzed video footage of one SPA, The World Down Under, enacted in three fifth‐grade classrooms. The video analysis revealed a wide variety of language demands that students encountered during the SPA, including a range of participant structures (whole class, small group, and individual), communicative modes (interpreting language, presenting language, and engaging interpersonally), and 18 different texts. Moreover, the language demands differed by classroom. A closer look at two video episodes illustrated how this more expansive communicative context afforded two English Learners (ELs) with opportunities to navigate the language demands of the assessment. However, the same language demands could present challenges for ELs with lower levels of English proficiency. The findings contribute to science education by bringing attention to the nature and role of language in SPAs, which has only begun to be described in the literature. © 2012 Wiley Periodicals, Inc. Sci Ed 96:631–651, 2012
An on‐site digital investigation methodology for data leak case
Authors: Lee Seokhee , Lee Keungi , Park Jong Hyuk , Lee Sangjin 
Published: January 2012

ABSTRACTThe ever growing storage device capacity poses a severe limit to the standard digital forensics collection procedures based on duplicating the original storage device by creating a bit‐by‐bit copy. Such a traditional procedure is followed, even if the goal of the investigation is to find a limited quantity of digital objects to support or refute an investigative hypothesis related to a precise case category. Therefore, in this paper, we propose a new methodology to deal with data leak cases, by applying an intelligent collection paradigm, a fast analysis approach that reduces investigation time. Copyright © 2012 John Wiley & Sons, Ltd.
Local modes in vibration–rotation spectroscopy
Authors: Jensen Per 
Published: January 2012

AbstractThe present paper is concerned with the description of highly excited rotational and/or vibrational states of molecules in terms of localized vibrations or local modes. Local mode effects are most common in molecules with multiple equivalent H–X bonds, which give rise to equivalent H–X local mode stretching vibrations, and the theory is outlined for the simplest such case, that of an H2X molecule. In the local mode picture of molecular vibration, experimentally observed, initially unexpected near‐degeneracies of vibrational states at high vibrational excitation, and of rotation–vibration states at high rotational excitation, can be explained in a relatively straightforward manner. The local mode theory predicts relations between the conventional rotation–vibration parameters whose values are determined in least‐squares fittings to observed rotation–vibration transition frequencies or wavenumbers. It should be emphasized, however, that such relations are valid only for particular forms of the effective rotation–vibration Hamiltonian used in the spectral analysis. We illustrate the theory with examples of experimental spectroscopic work where local mode effects play an important role in the interpretation of the experimental findings. The fact that local mode vibrations not only cause clustering of highly excited vibrational energy levels, but also of highly rotationally excited rotation–vibration energy levels, has been understood fairly recently. We outline the theoretical background for this phenomenon and relate it to the existing experimental work. © 2012 John Wiley & Sons, Ltd.This article is categorized under:

Theoretical and Physical Chemistry > Spectroscopy
Ab initio calculation of magnetic circular dichroism
Authors: Kjærgaard Thomas , Coriani Sonia , Ruud Kenneth 
Published: January 2012

AbstractWe discuss the ab initio calculation of all terms contributing to a magnetic circular dichroism (MCD) spectrum—i.e., A, B, and C terms—and give an overview of the most recent methods that have been introduced for calculating these different contributions. Among them, we highlight the use of the complex polarization propagator approach because it may give direct access to the A and B terms of MCD. We also briefly discuss how ab initio calculations for analyzing experimental MCD spectra can be used to gain insight into the electronic excited states of molecules. © 2012 John Wiley & Sons, Ltd.This article is categorized under:

Electronic Structure Theory > Ab Initio Electronic Structure Methods