bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular characterization, function, tissue differential expression, and single-nucleotide polymorphism of buffalo TP53 gene
Authors: Huang Lige , Sheng Dan , Fan Xinyang , Gao Ruixia , Miao Yongwang 
Published: May 2024

Abstract. TP53 has been shown to be involved in lactation in cattle. However, the role of TP53 in buffalo lactation remains unknown. To this end, we isolated and identified the complete coding sequence (CDS) of the TP53 gene from the buffalo mammary gland and further analyzed its molecular characteristics, function, tissue differential expression, and single-nucleotide polymorphism (SNP). A transcript of this gene was cloned with a CDS length of 1161 bp, encoding a protein consisting of 386 amino acid residues. Bioinformatics analysis showed that buffalo TP53 CDS and the physicochemical characteristics, conserved domains, structure, and function of its encoded protein are highly similar to those of other species in Bovidae. The buffalo TP53 protein contains an N-terminal activation domain, a DNA-binding domain, and a tetrameric domain, and it plays a functional role in the nucleus. TP53 was found to express in all 11 detected buffalo tissues, and its expression in the heart, kidney, brain, muscle, and rumen during lactation was significantly higher than that during non-lactation (p<0.05), while in the liver, lung, and mammary gland, its expression was the opposite (p<0.05). Interference experiments in buffalo mammary epithelial cells (BuMECs) showed that TP53 inhibits the expression of genes related to milk protein and milk fat synthesis through the PI3K–AKT–mTOR pathway. A synonymous nucleotide substitution (c.204C > T) was found in the TP53 CDS of river buffalo, which is the CC homozygote in swamp buffalo. The results indicate that the TP53 gene is involved in buffalo lactation by negatively regulating the synthesis of milk protein and milk fat.
MARKET INDICATORS OF CHARUTINHO (Hemiodus spp.) PICKLE
Authors: Santos Junior Luiz Monteiro dos , Almeida Bruno Kaleb dos Santos , Santos Paulo Roberto Brasil , Atayde Hérlon Mota 
Published: May 2024

Fresh samples of charutinho (Hemiodus spp.) were used to prepare four fish pickles formulations: F1 = 55v4s, F2 = 55v5s, F3 = 45v4s e F4 = 45v5s), which differ in the percentual content of alcohol vinegar and common iodized salt, indicated by numbers before the letters “v” and “s” of formulations code. They were analyzed by 78 untrained judges as following indicators and hedonic scale: acceptability (seven points), purchase intention (five points), and consumption frequency (mixed scale). Mostly, the judges accepted the formulations with minor vinegar content (F3 and F4). Of them, that with more salt content had the highest average. This same formulation has the best notes as purchase intention and consumption frequency. The charutinho pickle showed promising results by market indicators. The formulation with minor vinegar and more salt is better for entrepreneurial initiatives.
Effects of high dose of long-term ethanol exposure on oxidative damage of spleen in experimental rat model
Authors: Das Subir 
Published: May 2024

Background: Alcoholic beverages have been used in human societies at least since the beginning of recorded history. Research has demonstrated sufficiently to our understanding of the relation of drinking to specific disorders, and has shown that the relation between alcohol consumption and health outcomes is complex and multidimensional.
Methods: In this study we had demonstrated the role of oxidative stress in long-term ethanol induced spleen damage in 16-18 week-old male albino rats of Wistar strain weighing 200- 220 g.
Results: Ethanol exposure (1.6 g ethanol/ kg body wt/ day for 12 weeks) caused significant decrease in reduced glutathione (GSH) concentration and activities of superoxide dismutase and glutathione reductase; while significant increase in thiobarbituric acid reactive substances (TBARS) content and glutathione s-transferase activity in spleen homogenate.
Conclusion: Our study revealed that higher doses of ethanol (1.6 g/ kg body weight/ day) for a long period caused significant oxidative stress in the spleen. Intermediates of oxygen reduction may be associated with the development of ethanol induced organ damage.
YAPON SAFORASI DARAXTI VA LUPIN O‘SIMLIKLARINING ARALASHMASI TARKIBIDAGI MODDALARNING ANTIOKSIDANTLIK FAOLLIGINI ANIQLASH
Authors: Asqarov Ibrohimjon Rahmonovich , Mamatqulova Surayyoxon Abdusamatovna , Abdujabborova Charosxon Sanjarbek qizi 
Published: May 2024

Ushbu maqolada O‘zbekistonda uchraydigan Yapon saforasi (lat. Styphnolóbium japónicum) daraxti va Lupin (lat. Lupus) o‘simligi tarkibidagi biologik faol moddalarning kimyoviy tarkibi, shifobaxshlik xususiyatlari xamda xalq tabobatida, zamonaviy tibbiyotda qo‘llanilishi va biologik faolligini aniqlash maqsadida adrenalinning in vitro sharoitida autooksidlanish metodi yordamida mazkur o‘simlikning antioksidantlik faolligi aniqlandi. Antioksidantlik faolligini aniqlashdan maqsad.bu ikki o’simlikning aralashmasidan biologic faol oziq ovqat qo’shilmasi yaratish va amaliyotga tadbiq etish.
A práxis pedagógica na formação humana: um olhar a partir da perspectiva do projeto político pedagógico de escolas do campo e dos seus educadores (as)
Authors: Oliveira Filho Luis Moreira de , Lima Filipe Augusto Xavier , Vasconcelos John Weyne Maia , Batista José Wandsson do Nascimento , Ribeiro Luís Távora Furtado , Santana Joyce Gosta Gomes de , Cerqueira Gilberto Santos 
Published: May 2024

Este trabalho pretende realizar estudos sobre a práxis pedagógica na formação humana a partir da perspectiva de professores de escolas do campo de ensino médio. A pesquisa foi orientada pela abordagem qualitativa, tipologia exploratória descritiva, considerando o método materialismo histórico dialético. A aproximação com a realidade deu-se através de questionários semiestruturados, via google forms e pesquisa documental, ou seja, projeto político pedagógico de quatro escolas do campo de ensino médio. Os sujeitos da pesquisa foram professores de escolas do campo de tempo integral. A pergunta norteadora se deu da seguinte forma: o que revelam as falas sobre educação e práxis para a formação humana? Considerou-se a escola como espaços de lutas, formação mais ampla dos sujeitos e a sua organização como classe trabalhadora e como o trabalho pedagógico articula-se com a agroecologia.
Atividade larvicida do extrato etanólico das folhas frescas de Croton cordiifolius sobre Aedes aegypti e análise toxicológica em Mus musculus
Authors: Silva Marcilene Souza da , Araújo Alex Michel Silva , Silva Lidiane Quérolin Macena da , Barros Suelen Cristina Lourenço de , Cruz Rômulo Carlos Dantas da , Oliveira Marcela Albuquerque de , Arruda Marília Gabriela Muniz , Oliveira Maria Eduarda de Farias Albuquerque , Santos Patryck Érmerson Monteiro dos , Paiva Patrícia Maria Guedes , Souza Ivone Antonia de 
Published: May 2024

As plantas são compostas de substâncias bioativas que permitem a defesa contra insetos, dessa forma, se tornam importantes para produção de inseticidas naturais, os quais podem atuar no controle de mosquitos vetores de doenças, a exemplo de Aedes aegypti Linnaeus, 1762. No entanto, para estabelecer uma segurança na utilização de extratos botânicos, para formulação de inseticidas naturais, é necessário realizar estudos toxicológicos agudos utilizando a matéria-prima. Assim, o objetivo dessa pesquisa será avaliar a atividade inseticida do extrato etanólico bruto das folhas Croton cordiifolius sobre larvas de Aedes aegypti e toxicidade aguda em camundongos Mus musculus Linnaeus, 1758. O ensaio larvicida foi realizado de acordo com metodologia preconizada pela Organização Mundial da Saúde (WHO, 2005). Para a realização desses ensaios foram utilizadas 20 larvas de terceiro instar por repetição, as quais foram expostas a diferentes concentrações do extrato (14; 10; 7; 3,5; 1,35; e 0,87 mg mL-1). A toxicidade aguda seguiu o protocolo proposto pela OECD (2001), iniciando com a dose de 2000 mg mL-1 e administrada por via oral nos camundongos. O extrato etanólico de C. cordiifolius demonstrou atividade inseticida, causando 85% e 100% de mortalidade larval nas concentrações de 10 e 14 mg mL-1 respectivamente, após 48 horas de exposição. A toxicidade aguda teve uma DL50 estimada de ≥ 2000 mg kg-1, que foi classificada como categoria 5 (baixa de toxicidade). A partir dos conhecimentos gerados pode-se constatar o potencial larvicida e uma baixa toxicidade em mamíferos quando expostos ao extrato etanólico C. cordiifolius, demonstrando uma possível indicação de forma segura do uso do extrato vegetal para formulação de inseticidas naturais.
Gestão Social: os impactos da pandemia de COVID-19 na cidade de Vitória da Conquista sob a ótica do Cadastro Único (CadÚnico)
Authors: Oliveira Joabe Silva , Fagundes Marcus Vinicius Carvalho 
Published: May 2024

O presente artigo visa realizar uma análise comparativa dos impactos sociais gerados pela pandemia de Covid-19 no Município de Vitória da Conquista à luz dos dados do Cadastro Único – CadÚnico. Especificamente, busca comparar indicadores sociais do período pré-pandemia de Covid-19 (ano de 2019) com o período da pandemia de Covid-19 (anos de 2020 e 2021). Para tanto, é realizada uma pesquisa bibliográfica de abordagem descritiva e quali-quantitativa a partir de dados e informações do CadÚnico disponibilizadas na plataforma VIS DATA 3 beta do Ministério da Cidadania. O CadÚnico é um dos vários indicadores substanciais utilizados como instrumentos de medição da gestão pública, sobretudo, da Política de Assistência Social, as quais, desde a Constituição de 1988, busca, por meio de benefícios e serviços, afiançar os direitos sociais da população. Como principais resultados da pesquisa, constata-se, no período analisado, um agravamento das questões sociais no município de Vitória da Conquista, em razão do aumento de 35,39% das pessoas na condição social de “extrema pobreza”, além de um crescimento de 33,34% na quantidade de cidadãos inscritos no CadÚnico.
Propagação vegetativa por miniestaquia de Mimosa Tenuiflora (Mart.) Benth
Authors: Souza Cleyton dos Santos , Machado Evandro Luiz Mendonça , Titon Miranda , Silveira Gleydson Vinicius dos Santos , Botrel Rejane Tavares , Paiva Neto Emidio Estevam de , Gonzales Duberli Elera , Silva Maria Janaina Nascimento , Araújo Poliana Coqueiro Dias 
Published: May 2024

Objetivo do estudo foi verificar a possibilidade de propagação vegetativa por miniestacas de Mimosa tenuiflora, a necessidade de aplicação de ácido indolbutírico (AIB), o efeito da posição de remoção da miniestaca e determinar o tempo de enraizamento. A pesquisa foi realizada com indivíduos da espécie localizados principalmente na região semiárida do nordeste do Brasil. As sementes das árvores foram coletadas em quatro municípios do Estado do Rio Grande do Norte, com predomínio de solos Rezina e Cambissolo Eutrófico, com baixa profundidade, fertilidade e capacidade de retenção de água. A produtividade das miniestacas foi verificada a partir de um minijardim seminal. O enraizamento adventício da parte aérea foi testado utilizando-se diferentes concentrações de AIB e duas posições de remoção do propágulo. A dinâmica do enraizamento adventício foi estudada para determinar o momento em que ocorrem as alterações morfológicas responsáveis pela formação radicular. Os Principais Resultados encontrados demostram que a espécie tem boa capacidade de rebrota, tendo produzido em média 9,45 brotações por planta aos 75 dias. Há enraizamento adventício por miniestaca, independente do uso de AIB e da posição das miniestacas. O enraizamento das miniestacas inicia-se entre 5 e 10 dias após a estaca e foi concluído aos 25 dias, com enraizamento médio acima de 78%. A propagação vegetativa por miniestacas é viável. O uso de IBA não é necessário para enraizar as mini-estaca contudo, há melhores taxas de enraizamento com miniestacas intermediárias utilizando concentração de AIB entre 2000 e 6000 mg. L-1
Vitamina D y perfil lipídico en adultos de la ciudad de Babahoyo, año 2023
Authors: Chang-Tejada Lisseth Margarita , Zambrano-Macías Coralia 
Published: May 2024

La deficiencia de vitamina D se ha convertido en una condición de alta prevalencia mundial y se ha asociado con enfermedades cardiometabólicas incluyendo las dislipidemias. El objetivo fue analizar vitamina D y el perfil lipídico en adultos de la ciudad de Babahoyo, año 2023. Se realizó un estudio con diseño descriptivo-no experimental, de tipo analítico, transversal y retrospectivo, de enfoque cuantitativo. La muestra fue censal de 150 pacientes adultos atendidos durante el año 2023 en un laboratorio clínico privado de la Ciudad de Babahoyo, seleccionados bajo criterios de inclusión y exclusión. El protocolo fue aprobado por un Comité de Ética para Investigación en Seres Humanos. Se identificó 27,3% de déficit, 31,3% con insuficiencia y 41,3% con suficiencia de vitamina D en la población estudiada, mientras que el 66,0% presentó alteración de los componentes del perfil lipídico, a expensas de hipercolesterolemia, hipertrigliceridemia, niveles altos de colesterol de las lipoproteínas de baja densidad (LDL-C) y de concentraciones bajas de colesterol de las lipoproteínas de alta densidad (HDL-C), en su mayoría como dislipidemias mixtas. Se encontró una significativa y alta asociación entre la deficiencia de vitamina D con las alteraciones en el perfil de lípidos de los adultos de la ciudad de Babahoyo en el año 2023. Se aporta una evidencia más al importante papel que tiene la vitamina D en el metabolismo lipídico y se sientan las bases para el diseño de acciones de intervención en pro de la prevención del riesgo cardiometabólico en la población ecuatoriana.
Prevalence and Antibiotic Sensitivity Profile of Pseudomonas aeruginosa Isolated from Patients with Otitis Media in Kirkuk City
Authors: Hasan Golsan , Shakoor Obaid2 Siham , Mahmood Jassim3 Wafa 
Published: May 2024

Background: Otitis media (OM) is middle ear inflammation, it is a prevalent health care disorder that affects people of all ages and both genders. Pseudomonas aeruginosa (P. aeruginosa) is the most prevalent pathogen bacteria that causes the disease and has a high level of antibiotic resistance. The P. aeruginosa possess virulence characteristics which cause damages to the middle ear mucosa.Antibiotic resistance constitutes the main threat to people's health. Objective: The objective of the current study is to evaluate the frequency of P. aeruginosa isolated from Otitis media patients and investigate their antibiotic susceptibility pattern. Materials and Methods: total of 235 ear swabs were collected from otitis media patients who visited a specialized outpatient center in Kirkuk City and the ENT consultation facility at Kirkuk Hospitals. Antibiotic susceptibility testing by the VITEK 2 AST card and bacterial isolation and identification were both done. Results: The results of this study reveal that out of 235 ear swabs collected from patients with otitis media showed 22/235 (9.4 %) isolation rate for P. aeruginosa. And among the most effective antibiotics against the most of the detected pathogenic bacteria were Amikacin (95.4%), followed by Ceftazidime/ Avibactam, Piperacillin /Tazobactam, and Ceftolozane/ Tazobactam each with (91%), Colistin (86%), Imipenemand and Meropenem (81.8%, 86.4%) respectively, Ceftazidime, Cefeime, Levofloxacin, and Azreonam each with ( 77.3%), Ciprofloxacin and Ofloxacin both (72.7%) with Gentamicin (68.2%). Conclusion: The bacteria P. aeruginosa was most common prevalent isolate in the ear swabs, and this bacteria is multidrugs resistance (MDR). Additionally, the most efficient antibiotic against P. aeruginosa isolates responsible for otitis media was Amikacin.
The Correlational Study of the Quality of Students Enrolled in the University and Employability of Graduates from Selected Universities in South-Western Uganda
Authors: Milton Nuwabimpa 
Published: May 2024

The employability of university graduates is a concern for higher education stakeholders globally and Uganda is also concerned due to high unemployment rates post-graduation. Not much has been written about the relationship between quality of students enrolled in the university and their employability of graduates from selected Universities in South Western Uganda. This study aims to establish this relationship in selected universities in south western Uganda.
A correlational study collected data from 512 participants, including graduates, employers, university lecturers, administrators, and officers from quality assurance directorate of national council for Higher Education. Data on students discipline, grades, performance and employability were collected. SPSS and Structural equation modelling (SEM) version 21 was used to analyse associations between predictor and outcome variables.
The findings derived from conducting this research clearly demonstrate the presence of a significant statistical association between the quality of students enrolled in the university and employability of graduates at R= 0.589, R squared change 0.347, P =0.000
This suggests that by ensuring the quality of students enrolled in the University through discipline, students’ grades and performance increases the likelihood of producing graduates who are employable. It is very important that stakeholders in higher education make it a priority to keep a high standard of students and their moral fibre and this may facilitate their integration into the workforce.
Caso Clínico: Hydrops Fetal No Inmune
Authors: González González Luis Alberto , Zamora Chávez Zaida Selene , Mora Rivera Katya Miroslava , Jimenez Ahumada Maria Elizabeth , Tsuyuko Shiguematsu Maria 
Published: May 2024

Introducción: Se define hydrops fetalis como la presencia de 2 o más colecciones anormales de líquido en el feto, con evidencia mediante ecografía, 9, 11. Estas colecciones de líquido se presentan como ascitis, derrame pleural, derrame pericárdico y edema cutáneo generalizado (con espesor de piel >5 mm), 10, 12. Hay dos categorías de hydrops fetalis: inmune y no inmune. El hydrops fetalis no inmune es específicamente de casos sin aloinmunización con glóbulos rojos, 9. Actualmente se ha repostado que la incidencia de hydrops fetalis inmune a disminuido en los últimos años de manera significativa. El 90% de los casos de hydrops fetalis son de tipo no inmune. Presentación del caso: Se presenta el caso de embarazada con mal control prenatal con grupo sanguíneo O Rh negativo, no isoinmunizada, donde se decidió interrupción del embarazo por pérdida del bienestar fetal; al nacimiento se inició abordaje para hydrops fetalis no inmune. Conclusiones: Revisión del tema mediante discusión del abordaje diagnóstico y terapéutico actual.
Histoire et scandales chez saint Augustin
Authors: Haddad Joachim 
Published: May 2024

Saint Augustin n’est pas un auteur indifférent aux malheurs de son temps, comme le sac de Rome, qui apparaissent comme scandaleux et font douter bon nombre de ses fidèles. Ne témoignent-ils pas d’un abandon de Dieu, voire de son impuissance ? Augustin reprend, dans ses sermons, la notion de scandale afin de montrer qu’elle n’est pas adaptée pour définir ces événements malheureux. Il développe une réflexion sur l’histoire qui lui permet de donner un sens à ces apparents scandales, en les incluant dans l’histoire du salut sous la forme de l’épreuve. Cela suppose, pour le chrétien qui les subit, une herméneutique particulière.
An Economical, Green Pathway to Biaryls: Palladium Nanoparticles Catalyzed Ullmann Reaction in Ionic Liquid/ Supercritical Carbon Dioxide System
Authors: Cheng Jinsheng , Tang Longhua , Xu Jingying 
Published: December 2010

AbstractIn this paper, an economical, green pathway involving the palladium nanoparticles (Pd NPs) catalyzed reductive Ullmann reaction of an aryl chloride to afford a biaryl with high conversion and selectivity in an ionic liquid (IL)/supercritical carbon dioxide (ScCO2) system was developed. The combination of IL and ScCO2 provides superior advantages in product separation, catalyst recycling and reuse of reaction media over traditional organic solvents. Further investigations showed that the Brønsted‐acidic imidazolium IL {e.g., (1‐butyl‐3‐(sulfobutyl)‐imidazolium) hydrogen sulfate, [bmim(HSO3C4)][HSO4]}, can replace the traditional active hydrogen donor readily with much enhanced product separation efficiency, the use of IL also led to an obvious improved stability of the Pd NPs, which was very helpful for catalyst recycling. Carbon dioxide, a naturally abundant, non‐flammable, relatively non‐toxic, economical and recyclable “greenhouse” gas, was found to significantly promote the selectivity of the Pd NPs‐catalyzed aluminium‐induced reductive Ullmann reaction of aryl chlorides. Investigations showed that the Pd NPs catalyst and IL can be recycled for at least five runs, indicating the economic viability of this process.
Improved Synthesis of 1,2‐Bis(trimethylsilyl)benzenes using Rieke‐Magnesium or the Entrainment Method
Authors: Lorbach Andreas , Reus Christian , Bolte Michael , Lerner Hans‐Wolfram , Wagner Matthias 
Published: December 2010

Abstract1,2‐Bis(trimethylsilyl)benzene is the key starting material for the synthesis of efficient benzyne precursors and certain luminescent π‐conjugated materials. We now report that it can be conveniently prepared in tetrahydrofuran from 1,2‐dibromobenzene, chlorotrimethylsilane, and either Rieke‐magnesium (MgR) or magnesium turnings in the presence of 1,2‐dibromoethane as an entrainer (Mge). The most important advantages of these new protocols over the currently best‐established procedure (1,2‐dichlorobenzene, chlorotrimethylsilane, magnesium turnings, hexamethylphosphoramide) lie in the milder reaction conditions (MgR: 0 °C, 2 h; Mge: room temperature, 30 min vs. 100 °C, 2 days) and in the fact that the cancerogenic solvent hexamethylphosphoramide is avoided. Moreover, the improved protocols are also applicable for the high‐yield synthesis of 1,2,4,5‐tetrakis(trimethylsilyl)benzene, 4‐fluoro‐1,2‐bis(trimethylsilyl)benzene, 4‐chloro‐1,2‐bis(trimethylsilyl)benzene, and 4,5‐dichloro‐1,2‐bis(trimethylsilyl)benzene.
Stereoselective Tandem Epoxidation–Alcoholysis/Hydrolysis of Glycals with Molybdenum Catalysts
Authors: Marín Irene , Matheu M. Isabel , Díaz Yolanda , Castillón Sergio 
Published: December 2010

AbstractMolybdenum catalysts are efficient and selective catalysts for the tandem epoxidation/alcoholysis or epoxidation/hydrolysis of glucal and galactal derivatives. In glucal derivatives the selectivity is mainly controlled by the allylic substituent at position 3 of the glycal, obtaining in general the products derived from the initial formation of the α‐epoxide (gluco) when this hydroxy group is protected, while products derived from the β‐epoxide (manno) are mainly obtained when it is unprotected. In galactal derivatives the estereoselectivity is always high to give the α‐epoxide (galacto) and independent of the protecting groups.
Ruthenium Pincer‐Catalyzed Acylation of Alcohols Using Esters with Liberation of Hydrogen under Neutral Conditions
Authors: Gnanaprakasam Boopathy , Ben‐David Yehoshoa , Milstein David 
Published: December 2010

AbstractAcylation of secondary alcohols using non‐activated esters, in particular symmetrical esters (such as ethyl acetate), is achieved under neutral conditions with the liberation of molecular hydrogen. This unprecedented, environmentally benign reaction is homogenously catalyzed by a dearomatized ruthenium pincer PNN complex.
Highly Diastereoselective Indium‐Mediated Allenylation of N‐tert‐Butanesulfinyl Imino Ester: Efficient Synthesis of Optically Active α‐Allenylglycines
Authors: Jin Shen‐Shuang , Xu Ming‐Hua 
Published: December 2010

AbstractAn efficient and practical asymmetric synthesis of highly enantiomerically enriched α‐allenylglycines by room temperature indium‐mediated allenylation of chiral N‐tert‐butanesulfinyl imino esters with propargylic halides is described. The synthetic utilities of the approach were demonstrated by the rapid and convenient preparation of challenging cis‐substituted proline derivatives.