bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the International Study Group of Pancreatic Surgery definition of delayed gastric emptying after pancreatoduodenectomy in a high-volume centre
Authors: Welsch T , Borm M , Degrate L , Hinz U , Büchler M W , Wente M N 
Published: April 2010

Abstract

Background
Delayed gastric emptying (DGE) is a common complication after pancreatoduodenectomy. The International Study Group of Pancreatic Surgery (ISGPS) definition of DGE has not been evaluated and validated in a high-volume centre.

Methods
Complete data sets including assessment of gastric emptying were identified from a database of patients undergoing pancreatoduodenectomy between 2001 and 2008. Factors associated with DGE (grades A, B and C) were assessed by univariable and multivariable analyses.

Results
DGE occurred in 340 (44·5 per cent) of 764 patients. Median hospital stay was significantly prolonged in patients with DGE: 13, 21 and 40 days for grades A, B and C respectively versus 11 days for patients without DGE. DGE was associated with prolonged intensive care unit (ICU) admission (at least 2 days): 20·6, 28·6 and 61·8 per cent of those with grades A, B and C respectively versus 9·4 per cent of patients without DGE. Factors independently influencing DGE grade A were female sex, preoperative heart failure and major complications (grade III–V). Validation of the DGE definition revealed that DGE grades A and B were associated with interventional treatment in 20·1 and 44·4 per cent of patients.

Conclusion
The ISGPS DGE definition is feasible and applicable in patients with an uneventful postoperative course. Major postoperative complications and ICU treatment, however, might limit its usefulness. The identified risk factors for DGE are not amenable to perioperative improvement.
Marking the axilla with radioactive iodine seeds (MARI procedure) may reduce the need for axillary dissection after neoadjuvant chemotherapy for breast cancer
Authors: Straver M E , Loo C E , Alderliesten T , Rutgers E J T , Vrancken Peeters M T F D 
Published: April 2010

Abstract

Background
An important benefit of neoadjuvant chemotherapy is the increased potential for breast-conserving surgery. At present the response of axillary lymph node metastases to chemotherapy is not easily assessed, rendering axilla-conserving treatment difficult. The aim was to assess a new surgical method for evaluating the axillary response to chemotherapy.

Methods
Before neoadjuvant chemotherapy, proven tumour-positive axillary lymph nodes were localized using ultrasound-guided insertion of iodine-125-labelled (I-125) seeds. After neoadjuvant chemotherapy, the marked lymph nodes were removed selectively with the use of a γ probe. A complete axillary lymph node clearance was carried out to determine whether the pathological response in the marked node was indicative of that in the other lymph nodes.

Results
Tumour-positive axillary lymph nodes were localized successfully with I-125 seeds in 15 patients. The marked lymph node was detected and removed selectively after neoadjuvant chemotherapy in all patients. The pathological response to chemotherapy in the marked lymph node was indicative of the overall response in other removed lymph nodes.

Conclusion
This study showed that marking and selectively removing metastatic lymph nodes after neoadjuvant chemotherapy was feasible. The tumour response in the marked lymph node may be used to tailor further axillary treatment, making axilla-conserving surgery a possibility.
Value of indocyanine green clearance of the future liver remnant in predicting outcome after resection for biliary cancer
Authors: Yokoyama Y , Nishio H , Ebata T , Igami T , Sugawara G , Nagino M 
Published: April 2010

Abstract

Background
It is difficult to predict hepatic functional reserve accurately before major hepatectomy. The aim of this study was to analyse the usefulness of the future liver remnant plasma clearance rate of indocyanine green (ICGK-F, calculated as plasma clearance rate of indocyanine green (ICGK) × proportion of the future liver remnant) in predicting death after major hepatectomy.

Methods
Data on ICGK and ICGK-F were collected prospectively and analysed retrospectively for 274 patients who underwent right hepatectomy, right trisectionectomy or left trisectionectomy for biliary cancer between 1991 and 2008. The mortality rate and incidence of postoperative complications were analysed. Patients were separated into two groups according to year of operation (85 patients operated on between 1991 and 2000; 189 from 2001 to 2008).

Results
In multiple logistic regression analyses, an ICGK-F less than 0·05 had the strongest impact on the incidence of postoperative mortality (odds ratio 8·06; P &lt; 0·001). The postoperative mortality rate was significantly lower in the later period (P &lt; 0·001). In patients with an ICGK-F value between 0·040 and 0·049, the mortality rate in the early period was 30 per cent, whereas it was only 8 per cent in the later period.

Conclusion
An ICGK-F of 0·05 is a useful cut-off value for predicting mortality and morbidity. With careful perioperative patient management in an experienced institution, this cut-off value can be lowered further.
A quantitative method to evaluate mesenchymal stem cell lipofection using real‐time PCR
Authors: Ribeiro S.C. , Mendes R. , Madeira C. , Monteiro G.A. , da Silva C.L. , Cabral J.M.S. 
Published: April 2010

AbstractGenetic modification of human mesenchymal stem cells (MSC) is a powerful tool to improve the therapeutic utility of these cells and to increase the knowledge on their regulation mechanisms. In this context, strong efforts have been made recently to develop efficient nonviral gene delivery systems. Although several studies addressed this question most of them use the end product of a reporter gene instead of the DNA uptake quantification to test the transfection efficiency. In this study, we established a method based on quantitative real‐time PCR (RT‐PCR) to determine the intracellular plasmid DNA copy number in human MSC after lipofection. The procedure requires neither specific cell lysis nor DNA purification. The influence of cell number on the RT‐PCR sensitivity was evaluated. The method showed good reproducibility, high sensitivity, and a wide linear range of 75–2.5 × 106 plasmid DNA copies per cell. RT‐PCR results were then compared with the percentage of transfected cells assessed by flow cytometry analysis, which showed that flow cytometry‐based results are not always proportional to plasmid cellular uptake determined by RT‐PCR. This work contributed for the establishment of a rapid quantitative assay to determine intracellular plasmid DNA in stem cells, which will be extremely beneficial for the optimization of gene delivery strategies. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Pathoanatomy of posterior ankle impingement in ballet dancers
Authors: Russell Jeffrey A. , Kruse David W. , Koutedakis Yiannis , Mcewan Islay M. , Wyon Matthew A. 
Published: April 2010

AbstractDance is a high performance athletic activity that leads to great numbers of injuries, particularly in the ankle region. One reason for this is the extreme range of ankle motion required of dancers, especially females in classical ballet where the en pointe and demi‐pointe positions are common. These positions of maximal plantar flexion produce excessive force on the posterior ankle and may result in impingement, pain, and disability. Os trigonum and protruding lateral talar process are two common and well‐documented morphological variations associated with posterior ankle impingement in ballet dancers. Other less well‐known conditions, of both bony and soft tissue origins, can also elicit symptoms. This article reviews the anatomical causes of posterior ankle impingement that commonly affect ballet dancers with a view to equipping healthcare professionals for improved effectiveness in diagnosing and treating this pathology in a unique type of athlete. Clin. Anat. 23:613–621, 2010. © 2010 Wiley‐Liss, Inc.
Development of an industrial boiler virtual‐lab for control education using Modelica
Authors: Martin‐Villalba Carla , Urquia Alfonso , Dormido Sebastian 
Published: April 2010

AbstractThe use of the VirtualLabBuilder Modelica library and the Dymola modeling environment facilitates the implementation of virtual‐labs with elaborated user interfaces, and based on large and complex Modelica models. This implementation methodology is applied to develop an industrial boiler virtual‐lab for process control education. VirtualLabBuilder is freely available at www.euclides.dia.uned.es. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 21: 36–45, 2013
Disulfide chromophore and its optical activity
Authors: Maloň Petr , Bednárová Lucie , Straka Michal , Krejčí Lucie , Kumprecht Lukáš , Kraus Tomáš , Kubáňová Markéta , Baumruk Vladimír 
Published: April 2010

AbstractThe compounds I–IV derived from α‐D‐cyclodextrin moiety by bridging and/or interconnecting with various patterns of disulfide bonds were chosen as models for the spectroscopic study of conformation of the disulfide bridge. The energy gap between the disulfide and cyclodextrin's electronic transitions allows us to investigate absorption and electronic circular dichroism spectra without disturbing spectral overlaps with amides or aromatic amino acids in peptides or proteins. Raman optical activity (ROA) spectra were measured and the bands due to SS and CS stretching motion identified. Comparison with the quantum mechanical calculations of simple models indicates that sense of disulfide twist follows sign of the measured SS ROA band. Chirality 2010. © 2010 Wiley‐Liss, Inc.
Electronic and vibrational signatures of peptide helical structures: A tribute to Anton Mario Tamburro
Authors: Formaggio Fernando , Toniolo Claudio 
Published: April 2010

AbstractOur efforts on the synthesis of peptides with well‐characterized secondary structures, combined with detailed spectroscopic investigations, most of them performed in collaboration with internationally recognized experts, have allowed us to publish the electronic (electronic circular dichroism) and vibrational (FTIR absorption, vibrational circular dichroism, Raman, and Raman optical activity) signatures of the poorly studied peptide 310‐helix (and the related β‐bend ribbon spiral conformation as well) in comparison with those already known for the classical α‐helix. Chirality 2010. © 2010 Wiley‐Liss, Inc.
Der Einfluss von Kupfer (II) und Zink (II) auf die Bildung technischer Carbonatinkrustationen („Kesselstein”︁)
Authors: Zeppenfeld K. 
Published: April 2010

AbstractDer Einfluss von Cu2+‐ und Zn2+‐Ionen auf den Prozess der CaCO3‐Keimkristallbildung wurde in stark kalkhaltigem Wasser untersucht. Die Untersuchungen zeigen, dass Kupfer‐Ionen die Keimkristallbildung und die sich anschließende Kalkabscheidung wesentlich stärker verzögern als Zink‐Ionen. Wichtigster Grund hierfür ist die Bildung von ZnCO3‐Partikeln, die zu einer stark beschleunigten heterogenen CaCO3‐Keimkristallbildung führen.
Differences in disease presentation, management techniques, treatment outcome, and toxicities in African‐American women with early stage breast cancer treated with breast‐conserving therapy
Authors: Vicini Frank , Jones Pamela , Rivers Aeisha , Wallace Michelle , Mitchell Chris , Kestin Larry , Jaiyesimi Ishmael , Dekhne Nayana , Martinez Alvaro 
Published: April 2010

AbstractBACKGROUND:Data on patients who received breast‐conserving therapy (BCT) for early stage breast cancer were examined to detect differences in disease presentation, management techniques, long‐term treatment outcomes, and toxicities based on race.METHODS:Six hundred ninety‐nine women with breast cancer (39 African‐American [AA] women and 660 Caucasian [C] women) who received BCT were analyzed on race, clinical and pathologic characteristics at presentation, management techniques, treatment‐related toxicities, recurrence, and survival. The median follow‐up was 12.2 years.RESULTS:At diagnosis, AA women were younger (aged <50 years, 49% vs 29%; P = .002), had larger tumors (mean, 17.0 mm vs 13.9 mm; P = .032), had more estrogen receptor‐negative tumors (56% vs 18%; P < .001), and higher nuclear grade tumors (grade 3, 52% vs 29%; P = .006). Compared with C women, AA women more frequently received adjuvant chemotherapy (59% vs 19%; P < .001) and lymph node irradiation (26% vs 13%; P = .033). No other significant treatment differences were observed. After treatment, AA women experienced more breast pain (P = .001), more arm edema (P = .046), and less excellent cosmetic results (P = .008), but there were no statistically significant differences in local recurrence (P = .232), distant metastasis (P = .263), overall survival (P = .131), or cause‐specific survival (P = .092) based on race.CONCLUSIONS:The current results suggested that AA women present with larger and more aggressive breast tumors and, as a result, more frequently received adjuvant chemotherapy and lymph node irradiation. Small differences in treatment‐related toxicities and cosmesis were observed, but no differences in efficacy were identified. Cancer 2010. © 2010 American Cancer Society.
Acute and late onset cognitive dysfunction associated with chemotherapy in women with breast cancer
Authors: Wefel Jeffrey S. , Saleeba Angele K. , Buzdar Aman U. , Meyers Christina A. 
Published: April 2010

AbstractBACKGROUND:Growing evidence supports cognitive dysfunction associated with standard dose chemotherapy in breast cancer survivors. We determined the incidence, nature, and chronicity of cognitive dysfunction in a prospective longitudinal randomized phase 3 treatment trial for patients with T1‐3, N0‐1, M0 breast cancer receiving 5‐fluorouracil, doxorubicin, and cyclophosphamide with or without paclitaxel.METHODS:Forty‐two patients underwent a neuropsychological evaluation including measures of cognition, mood, and quality of life. Patients were scheduled to be assessed before chemotherapy, during and shortly after chemotherapy, and 1 year after completion of chemotherapy.RESULTS:Before chemotherapy, 21% (9 of 42) evidenced cognitive dysfunction. In the acute interval, 65% (24 of 37) demonstrated cognitive decline. At the long‐term evaluation, 61% (17 of 28) evidenced cognitive decline after cessation of treatment. Within this group of patients, 71% (12 of 17) evidenced continuous decline from the acute interval, and, notably, 29% (5 of 17) evidenced new delayed cognitive decline. Cognitive decline was most common in the domains of learning and memory, executive function, and processing speed. Cognitive decline was not associated with mood or other measured clinical or demographic characteristics, but late decline may be associated with baseline level of performance.CONCLUSIONS:Standard dose systemic chemotherapy is associated with decline in cognitive function during and shortly after completion of chemotherapy. In addition, delayed cognitive dysfunction occurred in a large proportion of patients. These findings are consistent with a developing body of translational animal research demonstrating both acute and delayed structural brain changes as well as functional changes associated with common chemotherapeutic agents such as 5‐flouorouracil. Cancer 2010. © 2010 American Cancer Society.
Evaluation of an 8‐color flow cytometric reference method for white blood cell differential enumeration
Authors: Cherian Sindhu , Levin Greg , Lo Wing Yin , Mauck Megan , Kuhn Donald , Lee Crystol , Wood Brent L. 
Published: April 2010

AbstractBackground:Manual microscopy is the current reference method for white blood cell (WBC) differential counts. However, manual counts are time and labor intensive, difficult in patients with low WBC counts, and can misclassify cells having difficult morphology. We investigated an 8‐color, single‐tube, lyse no‐wash flow cytometric method to perform an extended 8‐part differential as a potential replacement reference method for WBC differential enumeration.Methods:Whole blood was stained using a panel of antibodies including CD45APC‐Cy7, CD16+CD19FITC, CD33+CD64PE‐Cy5, CD123PE, HLA‐DRPE‐Cy7, CD34+CD117APC, and CD38A594 with the membrane permeant DNA binding dye Hoechst 34580 to generate an 8‐part differential (lymphocytes, granulocytes, monocytes, eosinophils, basophils, immature granulocytes, blasts, and nucleated RBCs) using TruCount beads to generate absolute counts for all populations. Manual and instrument differentials were generated for 300 blood samples ranging from normal to complex. Results were compared with the flow cytometric differential.Results:The flow cytometric WBC and differential correlated well with the Sysmex XE2100 hematology analyzer and gave comparable results to the manual differential. Areas of greatest discordance included enumeration of populations present at low numbers and misclassification of cells with unusual morphology by the manual method. This study describes a novel single‐tube flow cytometric method for performing a WBC count and 8‐part differential that performs well with both normal and difficult patient samples. These findings confirm the results of prior studies supporting the use of a flow cytometric differential as an improved reference method for the WBC differential and extend prior efforts by allowing positive identification of most cell populations. © 2010 International Clinical Cytometry Society
Probiotic Escherichia coli Nissle 1917 activates DC and prevents house dust mite allergy through a TLR4‐dependent pathway
Authors: Adam Emmanuelle , Delbrassinne Laurence , Bouillot Céline , Reynders Virginie , Mailleux Anne‐Catherine , Muraille Eric , Jacquet Alain 
Published: April 2010

AbstractExperimental animal and human studies have demonstrated that probiotic strains have beneficial effects on allergy. Here we report that the probiotic Escherichia coli Nissle 1917 strain (EcN) is able to activate DC, as shown by important cytokine synthesis together with up‐regulation of membrane expression of CD40, CD80 and CD86. This EcN‐induced DC activation was strictly dependent on the TLR4 signaling pathway and was also associated with stimulation of NF‐κB and MAPK. We next investigated the prophylactic potential of i.n. co‐administration of EcN with a recombinant form of Der p 1 (ProDer p 1) in a murine model of mite allergy. I.n. vaccinations with EcN plus ProDer p 1 prevented the subsequent allergic response following Der p 1 sensitization and airway challenge with aerosolized mite extracts through the induction of an allergen‐specific IgG2a response, the prevention of specific IgE production and a strong reduction of IL‐5 secretion by allergen‐restimulated splenocytes. EcN alone or in combination with ProDer p 1 inhibited the development of airway eosinophilia and neutrophilia. This in vivo protective effect of EcN was, in part, mediated by TLR4 signaling. Our results suggest that EcN represents an efficient adjuvant to prevent allergic responses.
Generation of robust CD8+ T‐cell responses against subdominant epitopes in conserved regions of HIV‐1 by repertoire mining with mimotopes
Authors: Schaubert Keri L. , Price David A. , Salkowitz Janelle R. , Sewell Andrew K. , Sidney John , Asher Tedi E. , Blondelle Sylvie E. , Adams Sharon , Marincola Francesco M. , Joseph Aviva , Sette Alessandro , Douek Daniel C. , Ayyavoo Velpandi , Storkus Walter , Leung Ming‐Ying , Ng Hwee L. , Yang Otto O. , Goldstein Harris , Wilson Darcy B. , Kan‐Mitchell June 
Published: April 2010

AbstractHLA‐A*0201‐restricted virus‐specific CD8+ CTL do not appear to control HIV effectively in vivo. To enhance the immunogenicity of a highly conserved subdominant epitope, TV9 (TLNAWVKVV, p24 Gag19–27), mimotopes were designed by screening a large combinatorial nonapeptide library with TV9‐specific CTL primed in vitro from healthy donors. A mimic peptide with a low binding affinity to HLA‐A*0201, TV9p6 (KINAWIKVV), was studied further. Parallel cultures of in vitro‐primed CTL showed that TV9p6 consistently activated cross‐reactive and equally functional CTL as measured by cytotoxicity, cytokine production and suppression of HIV replication in vitro. Comparison of TCRB gene usage between CTL primed from the same donors with TV9 or TV9p6 revealed a degree of clonal overlap in some cases and an example of a conserved TCRB sequence encoded distinctly at the nucleotide level between individuals (a “public” TCR); however, in the main, distinct clonotypes were recruited by each peptide antigen. These findings indicate that mimotopes can mobilize functional cross‐reactive clonotypes that are less readily recruited from the naïve T‐cell pool by the corresponding WT epitope. Mimotope‐induced repertoire diversification could potentially override subdominance under certain circumstances and enhance vaccine‐induced responses to conserved but poorly immunogenic determinants within the HIV proteome.
Multiple roles of ERCC1‐XPF in mammalian interstrand crosslink repair
Authors: Rahn Jennifer J. , Adair Gerald M. , Nairn Rodney S. 
Published: April 2010

AbstractDNA interstrand crosslinks (ICLs) are among the most deleterious cytotoxic lesions encountered by cells, mainly due to the covalent linkage these lesions create between the two strands of DNA which effectively blocks replication and transcription. Although ICL repair in mammalian cells is not fully understood, processing of these lesions is thought to begin by “unhooking” at the site of the damaged base accompanied by the generation of a double strand break and ultimately repair through translesion synthesis and homologous recombination. A key player in this repair process is the heterodimeric protein complex ERCC1‐XPF. Although some models of ICL repair restrict ERCC1‐XPF activity to the unhooking step, recent data suggest that this protein complex acts in additional downstream steps. Here, we review the evidence implicating ERCC1‐XPF in multiple steps of ICL repair. Environ. Mol. Mutagen., 2010. © 2010 Wiley‐Liss, Inc.
Numerical modelling of aeolian erosion over rough surfaces
Authors: Turpin C. , Badr T. , Harion J.‐L. 
Published: April 2010

AbstractThe presence of non‐erodible roughness elements on erodible surfaces has the effect of absorbing part of the wind shear stress and thus protecting the erodible surface from wind erosion. This paper examines the shear stress distribution over roughness arrays of varying density, representing the progress of erosion on a bed of erodible and non‐erodible particles. Three‐dimensional numerical simulations, simulating wind flow over a bed of particles covered by roughness elements, were conducted in order to investigate the effect of roughness elements on the shear stress near the surface. The results of these simulations confirm that the erosion of soil by wind is strongly attenuated by the presence of roughness elements on the surface and depends on the geometric properties of the roughness elements. Based on the new numerical results obtained, a refinement of existing theoretical approaches is developed to describe the dependence of the friction velocity upon roughness frontal area and real exposed cover rate. The new formulation proposed will allow a more accurate evaluation of shear stress partitioning as a function of topographic changes. Copyright © 2010 John Wiley & Sons, Ltd.