bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of Immune Synapse by Altered Dendritic Cell Actin Distribution: A New Pathway of Mesenchymal Stem Cell Immune Regulation
Authors: Aldinucci Alessandra , Rizzetto Lisa , Pieri Laura , Nosi Daniele , Romagnoli Paolo , Biagioli Tiziana , Mazzanti Benedetta , Saccardi Riccardo , Beltrame Luca , Massacesi Luca , Cavalieri Duccio , Ballerini Clara 
Published: October 2010

AbstractImmune synapse formation between dendritic cells (DCs) and T cells is one of the key events in immune reaction. In immunogenic synapses, the presence of fully mature DCs is mandatory; consequently, the modulation of DC maturation may promote tolerance and represents a valuable therapeutic approach in autoimmune diseases. In the field of cell therapy, bone marrow mesenchymal stem cells (MSCs) have been extensively studied for their immunoregulatory properties, such as inhibiting DC immunogenicity during in vitro differentiation and ameliorating in vivo models of autoimmune diseases (e.g., experimental allergic encephalomyelitis). MSCs seem to play different roles with regard to DCs, depending on cell concentration, mechanism of stimulation, and accompanying immune cells. The aim of this work was to elucidate the immunogenic effects of MSC/DC interactions during DC activation (LPS stimulation or Ag loading). Human monocyte-derived DCs, bone marrow-derived MSCs, and circulating lymphocytes obtained from healthy donors, as well as the laboratory-generated influenza virus hemagglutinin-derived peptide, aa 306–318 peptide-specific T cell line were used for this study. We demonstrate that MSCs mediate inhibition of DC function only upon cell–cell contact. Despite no modification observed in cell phenotype or cytokine production, MSC-treated DCs were unable to form active immune synapses; they retained endocytic activity and podosome-like structures, typical of immature DCs. The transcriptional program induced by MSC–DC direct interaction supports at the molecular pathway level the phenotypical features observed, indicating the genes involved into contact-induced rearrangement of DC cytoskeleton.
Hydrogel-Delivered GM-CSF Overcomes Nonresponsiveness to Hepatitis B Vaccine through the Recruitment and Activation of Dendritic Cells
Authors: Chou Ho-Yuan , Lin Xi-Zhang , Pan Wen-Yu , Wu Ping-Yi , Chang Chia-Ming , Lin Tsai-Yu , Shen Hsin-Hsin , Tao Mi-Hua 
Published: October 2010

AbstractThe standard hepatitis B surface Ag (HBsAg) vaccine fails to induce anti-hepatitis B surface Abs in 5–10% of healthy subjects, a phenomenon known as HBsAg nonresponsiveness, which is closely related to HLA class II alleles and impaired Th cell responses to HBsAg in these subjects. We hypothesized that GM-CSF, a potent adjuvant in enhancing the Ag-presentation activity of APCs, might help to generate Th cell responses in nonresponders, subsequently providing help for B cells to produce anti-hepatitis B surface Abs. We used a thermosensitive biodegradable copolymer (hydrogel) system to codeliver HBsAg and GM-CSF to achieve maximal local cytokine activity at the injection site. In responder mouse strains, hydrogel-formulated HBsAg plus GM-CSF (Gel/HBs+GM) vaccine elicited much greater anti-hepatitis B surface Ab titers and Th cell proliferative responses than a commercial aluminum-formulated HBsAg vaccine or free HBsAg. The adjuvant effect of the Gel/HBs+GM vaccine was dependent upon the local release of GM-CSF. More importantly, the Gel/HBs+GM vaccine elicited high HBsAg-specific Ab titers and Th cell responses in B10.M mice, a mouse strain that does not respond to the current HBsAg vaccine because of its H-2 haplotype. Analysis of the draining lymph nodes of Gel/HBs+GM vaccine-treated mice revealed an elevated number of CD11c+ dendritic cells showing enhanced expression of MHC class II and a variety of costimulatory molecules. These results demonstrate that hydrogel-formulated GM-CSF might represent a simple and effective method to generate next-generation hepatitis B virus vaccines for inducing anti-hepatitis B surface Abs in nonresponders.
The Vibrant Soundbridge
Authors: Pusalkar Ashutosh G 
Published: October 2010

Abstract
Till about 15 years ago, the only choice of hearing improvement for moderate sensorineural hearing loss with severe speech discrimination defect was a hearing aid. It was only after Mr. Geoff Ball, an electronic engineer who was suffering from a similar defect, started thinking of an alternative to the conventional hearing aid that the Vibrant Soundbridge came into existence, and with the passage of time the indications for the use of the same have increased.
Men's memory for women's sexual‐interest and rejection cues
Authors: Treat Teresa A. , Viken Richard J. , Kruschke John K. , McFall Richard M. 
Published: October 2010

AbstractThe current work characterizes young men's memory for young women's heterosocially relevant affective cues (e.g. sexual interest and rejection) and examines characteristics of both the woman being perceived and the male perceiver as predictors of memory. Undergraduate men (n = 232) completed similarity‐ratings and recognition‐memory tasks with photos of undergraduate women who varied in attractiveness, provocativeness of clothing and expression of sexual interest. Participants also completed a control memory task and a measure relevant to the risk of exhibiting sexually aggressive behaviour, as well as indicating how many serious relationships they had experienced. Multilevel regression techniques revealed that (a) men's memory for women's sexual interest improved when women were sexually interested at encoding, attractive and dressed provocatively; (b) men who reported more frequent serious relationships showed better memory for women's sexual interest and (c) men at risk of exhibiting sexually aggressive behaviour showed worse memory for women's sexual interest. Copyright © 2010 John Wiley & Sons, Ltd.
High Degree of Optical Tunability of Self‐Assembled Photonic‐Plasmonic Crystals by Filling Fraction Modification
Authors: López‐García Martín , Galisteo‐López Juan F. , Blanco Álvaro , López Cefe , García‐Martín Antonio 
Published: October 2010

AbstractThe optical properties of two‐dimensional hybrid photonic‐plasmonic crystals are fine‐tuned by modifying the dielectric component of the system. The filling fraction of the dielectric component in monolayers of spheres deposited on gold substrates is controlled by means of oxygen‐plasma etching. Doing so enables spectral tuning of the optical modes of the system. Experiments are performed on both optically passive and active samples showing the possibility for strong modification of the emission properties of samples containing emitters distributed within the spheres. The change in sphere diameter needed to substantially modify the sample's optical response points to a potential use of these samples as sensors or tunable emitting devices if appropriate polymeric components are employed.
Brief Communication: Shape analysis of the MT 1 proximal articular surface in fossil hominins and shod and unshod Homo
Authors: Proctor Daniel J. 
Published: October 2010

AbstractAs a follow‐up study to Proctor et al. (Am J Phys Anthropol 135 (2008) 216–224), this study quantifies the first metatarsal proximal articular surface using three‐dimensional morphometrics to test for differences in articular surface shape between habitually shod and habitually unshod humans. In addition, differences in shape between Homo, Pan, Gorilla, and Hylobates are compared to the fossil hominin specimens A. L. 333‐54, Stw 562, Stw 573 (“Little Foot”), OH 8, SKX 5017, and SK 1813. No difference in surface shape was found between habitually shod and habitually unshod humans. There is a clear quantitative division in articular surface shape between humans and apes that is more pronounced than a previous study by Proctor et al. (Am J Phys Anthropol 135 (2008) 216–224), due to additional landmarks present in this study. The specimen OH 8 is indistinguishable from modern Homo. The fossils A. L. 333‐54, Stw 562, and Stw 573 are intermediate in shape between humans and apes. The specimens SKX 5017 and SK 1813 have a more apelike articular surface. When combined with other characteristics, this trait suggests that Paranthropus used a degree of abduction during locomotion that was much less than that in extant apes, but greater than that in Australopithecus, allowing for some small degree of grasping ability. Am J Phys Anthropol, 2010. © 2010 Wiley‐Liss, Inc.
Bayesian predictive travel time methodology for advanced traveller information system
Authors: Khan Ata M. 
Published: October 2010

AbstractTravellers can benefit from the availability of point‐to‐point driving time estimates on a real time basis for making travel decisions such as route choice at strategic locations (e.g. junctions of major routes). This paper reports a predictive travel time methodology that features a Bayesian approach to fusing and updating information for use in advanced traveller information system. The methodology addresses the issue that data captured in real time on travel conditions becomes obsolete and has archival value only unless it is used as an input to a predictive travel time method for updating the information. The need for fusing real time data with other factors that influence travel time is defined and the concept of predictive travel time is discussed. The methodological framework and its components are advanced and an example application is provided for illustrating the fusion of data captured by infrastructure‐based and mobile technology with model‐based predictions in order to produce expected travel times. Copyright © 2010 John Wiley & Sons, Ltd.
Probabilistische Bemessung von geklebten Anschlüssen im Holzbau
Authors: Tannert Thomas , Hehl Simon , Vallée Till 
Published: October 2010

AbstractKleben von tragenden Bauteilen ist im Holzbau auf wenige Anwendungsgebiete beschränkt. Es wird hauptsächlich zur Herstellung von Brettschichtholz oder zur Ertüchtigung bestehender Strukturen eingesetzt. Um Kleben als Alternative zu stiftförmigen mechanischen Verbindungsmitteln einzusetzen, muss die Bemessung geklebter Anschlüsse ermöglicht werden. Holzspezifische Faktoren wie Anisotropie, Streuung der mechanischen Eigenschaften und sprödes Versagen erschweren eine genaue Vorhersage. Aufgrund der auftretenden Spannungsspitzen ist ein deterministischer Spannungsnachweis nicht geeignet. Eine Alternative zur Bestimmung der Tragfähigkeit geklebter Anschlüsse bietet die in diesem Beitrag vorgestellte probabilis tische Methode, die es erlaubt, die Spannungsverteilungen in geklebten Verbindungen mit ihren Spannungsspitzen und die Streuungen der Festigkeitswerte des Werkstoffes zu erfassen. Im Rahmen der vorgestellten Arbeit wurden geklebte Doppel‐Laschenanschlüsse sowohl experimentell als auch numerisch betrachtet, wobei der Einfluss der überlappungslänge, der Klebschichtdicke und des Klebstoff‐E‐Moduls betrachtet wurde. Im Anschluss wurde ein probabilistisches Bemessungsverfahren angewendet und eine gute übereinstimmung zwischen experimentellen und theoretischen Traglasten erreicht. Das Verfahren kann unmittelbar für die Bemessung geklebter Anschlüsse angewandt werden.Probabilistic design of adhesively bonded timber joints. The use of adhesive bonding in structural timber engineering is limited to the production of laminated wood and the toughening of existing structures. To use adhesives as an alternative to mechanical fasteners, an accurate and reliable design method has to be developed. Characteristic material properties of wood such as anisotropy, large scattering of mechanical values and brittle failure complicate the capacity prediction of bonded joints. A deterministic approach is not able to deal with the stress peaks inside the joints. With the probabilistic concept, an alter native solution is presented, which allows for a strength prediction under consideration of the complex stress distributions and the stress peaks in the joint, as well as the scatterings of the mechanical properties of the material. Adhesively bonded double lap joints were investigated both experimentally and numerically under consideration of various parameters such as overlap length, adhesive layer thickness and type of adhesive. The probabilistic design method was applied and good agreement between experimental and theoretical failure loads was obtained. The proposed method has immediate actionable application for the dimensioning of adhesively bonded timber joints.
Orientation of paramecium swimming in a static magnetic field: Dependence on membrane lipid fluidity
Authors: Nakaoka Yasuo , Itoh Junya , Shimizu Kikuo 
Published: October 2010

AbstractWe studied the swimming orientation of the ciliated protozoan Paramecium aurelia in a static magnetic field (0.78 T). P. aurelia is a complex of species termed syngens, whose cell morphology appears similar on microscopic examination. In the magnetic field, the cells of some syngens gradually changed their swimming orientation so that they were swimming perpendicular or parallel to the magnetic field, although such sensitivity to magnetic fields differs between syngens. When the temperature of the cell suspension was raised, the magnetic sensitivity of the cells was decreased. On the other hand, when the cells were cultured beforehand at a high temperature, their magnetic sensitivity was increased. These results raise the possibility that membrane lipid fluidity, which is inversely proportional to the membrane lipid order, contributes to the magnetic orientation of syngens. In this study, measurements of membrane lipid fluidity obtained using fluorescence image analysis with the lipophilic dye, laurdan (6‐lauroyl‐2‐dimethylaminonaphtalene), showed that the degree of membrane lipid fluidity was correlated with the differences in magnetic orientation between syngens. That is, the syngens with decreased membrane fluidity showed an increased degree of magnetic orientation. Therefore, the membrane lipid order is a key factor in the magnetic orientation of Paramecium swimming. Bioelectromagnetics 32:66–72, 2011. © 2010 Wiley‐Liss, Inc.
Bifurcated aortic endograft limb occlusion managed with a novel method of isolated pharmacomechanical thrombectomy
Authors: Pappy Reji , Hanna Elias B. , Hennebry Thomas A. 
Published: October 2010

AbstractLimb occlusion of an aortic endograft is uncommon, however, it can result in permanent sequelae if management is delayed. We report the first case utilizing the Trellis device to achieve localized and prompt isolated pharmacomechanical thrombectomy (PMT) for the treatment of acute limb thrombosis of a bifurcated aortic endograft. © 2010 Wiley‐Liss, Inc.
A Computational Investigation on the Flow of Non‐Newtonian Polymers in Safety Valves
Authors: Moncalvo D. , Friedel L. , Jörgensen B. 
Published: October 2010

AbstractAt the moment, very little knowledge is available about the flow of shear‐thinning media in safety valves. In this paper, the flow of aqueous solutions of polyvinylpyrrolidone with a zero‐shear viscosity between 0.3 and 3 Pa s in a LESER Type 441 DN 25/40 safety valve with a lift of 2.2 mm for relieving pressures up to 6.5 bar is computed using ANSYS FLUENT 12. According to these calculations, the lowest values of the dynamic viscosity are reached in the slit between the seat and the disk due to the large local velocity gradients. A more moderate increment in the fluidity of the solutions is observed also in the recirculation zone outside the disk when the stream from the rear of the valve meets the one from the front. A preliminary study suggests that the experimental flows may be turbulent in the slit and then evolve into a laminar profile in the discharge pipe. For this reason, the calculations are carried out assuming laminar, turbulent and transitional flows. The predicted mass flow rates are close to each other and to the measured values, except at relieving pressures close to ambient. The major cause of deviation seems to be the entrainment of air microbubbles, whose elongation may be responsible for additional shearing in the solutions with large polymer weight.
Development and Monitoring of a Continuous Polyurethane Pilot Reactor
Authors: Nadjiadjim G. , Sheibat‐Othman N. , Févotte G. , Dupuy J. 
Published: October 2010

AbstractThe transition from batch to continuous polyurethane (PU) process is considered for process intensification. An integrated methodological and technological study is proposed. The development of the new process is based on modeling and product characteristics. In order to do so, the kinetics of the polyaddition of polycaprolactone diol (polyol) and 4,4'‐diphenylmethane diisocyanate were studied and identified by using offline measurement of the diisocyanate concentration. Some characteristics of the final polymer were also investigated, such as the heat capacity and viscosity that affect the behavior of the continuous process. These results were used to design a tubular reactor for continuous production of PU. Since online monitoring of intensified processes is primordial, in situ near‐infrared spectroscopy was used to predict the concentration of free diisocyanate in real time.
Recovery of Valuable Metals from Battery Scrap
Authors: Vest M. , Georgi‐Maschler T. , Friedrich B. , Weyhe R. 
Published: October 2010

AbstractEs wurden Recyclingkonzepte für Nickel‐Cadmium‐Batterien, Nickel‐Metallhydrid‐Batterien, Primär‐Gerätebatterien und Li‐Ionen‐Gerätebatterien entwickelt. Einige dieser Verfahren sind bereits industriell umgesetzt und als Best Available Technology (BAT) bewertet worden. In diesem Beitrag werden zunächst die entwickelten Verfahren und ihre Möglichkeiten zum Erreichen einer optimalen Recyclingeffizienz entsprechend der aktuellen nationalen und europäischen Gesetzgebung vorgestellt. Anschließend werden die Potenziale des aktuellen Forschungsprojektes zum Recycling von Li‐Ionen‐Batterien aus dem Fahrzeugsektor erörtert.
Protein tyrosine phosphatase activity is necessary for E‐cadherin‐activated Src signaling
Authors: McLachlan Robert W. , Yap Alpha S. 
Published: October 2010

AbstractCo‐operation between cadherin adhesion molecules and the cytoskeleton is a key aspect of tissue morphogenesis that is mediated by cortical signaling at adhesive junctions. One such signal is the non‐receptor tyrosine kinase, Src, which acts in several pathways at epithelial junctions, including E‐cadherin signaling itself. We now present two new insights into junctional Src signaling. Firstly, we report that upstream protein tyrosine phosphatase (PTP) activity is required to stimulate E‐cadherin‐activated Src signaling at junctions. Perturbing PTP activity with vanadate selectively reduced the activity of Src tyrosine kinases at junctions. Moreover, E‐cadherin homophilic ligation could not stimulate Src signaling in vanadate‐treated cells. Additionally, vanadate treatment phenocopied the effects of Src inhibition on the actin cytoskeleton, suggesting that PTP activity is required for the dynamic regulation of the actin cytoskeleton by cadherin‐activated Src signaling. Secondly, we identified a role for PTP‐activated Src signaling in supporting apical junctional tension by targeting non‐muscle myosin IIB. The linear shape of the apical junctions was lost in PTP‐ and Src‐inhibited cells, and inhibiting Src selectively affected the junctional localization of myosin IIB but not of myosin IIA. We conclude that PTP‐activated Src signaling is a possible upstream regulator of myosin IIB at the epithelial zonula adherens. © 2010 Wiley‐Liss, Inc.
Marijuana use and testicular germ cell tumors
Authors: Trabert Britton , Sigurdson Alice J. , Sweeney Anne M. , Strom Sara S. , McGlynn Katherine A. 
Published: October 2010

AbstractBACKGROUND:Since the early 1970s, the incidence of testicular germ cell tumors (TGCTs) in the United States has been increasing; however, potential environmental exposures accounting for this increase have not been identified. A previous study reported a significant association between frequent and long‐term current marijuana users and TGCT risk. The purpose of this study was to evaluate the relation between marijuana use and TGCTs in a hospital‐based case‐control study.METHODS:TGCT patients diagnosed between January 1990 and October 1996 (n = 187) and male friend controls (n = 148) were enrolled in the study. All participants were between the ages of 18 and 50 at the time of diagnosis and resided in Texas, Louisiana, Arkansas, or Oklahoma. Associations of marijuana use and TGCTs were estimated using unconditional logistic regression, adjusting for age, race, prior cryptorchidism, cigarette smoking, and alcohol intake.RESULTS:Overall, patients with TGCTs were more likely to be frequent marijuana users (daily or greater) compared with controls (odds ratio [OR], 2.2; 95% confidence interval [CI], 1.0‐5.1). Histological‐specific analyses revealed that patients with nonseminoma were significantly more likely than controls to be frequent users (OR, 3.1; 95% CI, 1.2‐8.2) and long‐term (≥10 years) users (OR, 2.4; 95% CI, 1.0‐6.1).CONCLUSIONS:The finding of an association between frequent marijuana use and TGCTs, particularly among men with nonseminoma, was consistent with the findings of a previous report. Additional studies of marijuana use and TGCTs are warranted, especially studies evaluating the role of endocannabinoid signaling and cannabinoid receptors in TGCTs. Cancer 2011. © 2010 American Cancer Society.
Effect of isomorphic atom substitution on the refractive index and oscillator parameters of TlInS2xSe2(1‐x) (0.25 ≤ x ≤ 1) layered mixed crystals
Authors: Gasanly N. M. 
Published: October 2010

AbstractThe optical properties of TlInS2xSe2(1‐x)mixed crystals (0.25 ≤ x ≤ 1) have been studied at room temperature through the transmittance and reflectivity measurements in the wavelength range of 400–1100 nm. The spectral dependence of the refractive index for all compositions of studied crystals were obtained. The dispersion of the refractive index is discussed in terms of the Wemple–DiDomenico single‐effective‐oscillator model. The compositional dependencies of refractive index dispersion parameters: oscillator energy, dispersion energy and zero‐frequency refractive index were revealed. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Dynamic Lkb1‐TORC1 signaling as a possible mechanism for regulating the endoderm‐intestine transition
Authors: Marshall Kathryn E. , Tomasini Amber J. , Makky Khadijah , N. Kumar Suresh , Mayer Alan N. 
Published: October 2010

AbstractThe intestinal epithelium arises from undifferentiated endoderm via a developmental program known as the endoderm‐intestine transition (EIT). Previously we found that the target of rapamycin complex 1 (TORC1) regulates intestinal growth and differentiation during the EIT in zebrafish. Here we address a possible role for the tumor‐suppressor kinase Lkb1 in regulating TORC1 in this context. We find that TORC1 activity is transiently upregulated during the EIT in both zebrafish and mouse. Concomitantly, Lkb1 becomes transiently localized to the nucleus, suggesting that these two phenomena may be linked. Morpholino‐mediated knockdown of lkb1 stimulated intestinal growth via upregulation of TORC1, and also induced precocious intestine‐specific gene expression in the zebrafish gut epithelium. Knockdown of tsc2, which acts downstream of lkb1, likewise induced early expression of intestine‐specific genes. These data suggest that programmed localization of Lkb1 could represent a novel mechanism for regulating the EIT during intestinal development in vertebrates. Developmental Dynamics 239:3000–3012, 2010. © 2010 Wiley‐Liss, Inc.