bluuebunny/crossref_metadata_2025_split huggingface dataset:
Trends of contact allergy to fragrance mix I and Myroxylon pereirae among Danish eczema patients tested between 1985 and 2007*
Authors: Thyssen Jacob P. , Carlsen Berit Christina , Menné Torkil , Johansen Jeanne Duus 
Published: August 2010

Background:  Fragrance contact allergy has for long been recognized as an important health issue. In Denmark, the frequency of fragrance mix (FM) I contact allergy increased between 1985–1986 and 1997–1998 among male and female dermatitis patients.Objectives:  To investigate the development of FM I and Myroxylon pereirae (MP) contact allergy between 1985 and 2007 with an emphasis on recent years.Methods:  A retrospective analysis of all patch test data from our database was performed. Comparison of sensitivity rates was made using a chi‐squared test for trend. Logistic regression analyses were used to test for associations.Results:  Of 16 173 patients, 7.2% were sensitized to FM I and 4% to MP. FM I contact allergy was associated with female sex [odds ratio (OR) = 1.52; 95% confidence interval (CI) = 1.33–1.74] and age between 41 and 60 years (OR = 3.20; 95% CI = 1.98–5.21). Significant declines of FM I and MP reactions among women but not men were observed between 1999 and 2007.Conclusions:  Although the frequency of FM I contact allergy has decreased in Denmark in recent years, it is still high. Furthermore, fragrance contact allergy is caused by other important allergens not included in this analysis. Allergic contact reactions to the ingredients of FM I remain a problem in European consumers.
Clinical scoring and biophysical evaluation of nasolabial skin barrier damage caused by rhinorrhea
Authors: Houben Evi , Adam Ralf , Hachem Jean‐Pierre , Roseeuw Diane , Rogiers Vera , De Paepe Kristien 
Published: August 2010

Background:  An acute viral cold is a very common illness and is characterized by sneezing and a runny nose. Because of rhinorrhea and frequent use of handkerchiefs, the skin around the nose feels uncomfortably dry and flaky.Objectives/Methods:  To evaluate the nasolabial skin barrier impairment, 14 female volunteers with a common cold were recruited. Visually assessed clinical scoring and/or biophysical measurements – including transepidermal water loss, stratum corneum hydration, skin colour, squamometry, skin pH, and a skin surface lipid profile analysis – were carried out at the start of the cold, a second time when the severity of the cold symptoms was maximal, and finally when the volunteers felt healthy again and stopped using handkerchiefs.Results and Conclusions:  Transepidermal water loss assessments showed significantly higher measurements on the maximum outcome of the nasal cold compared with the time‐point when the symptoms of the cold had disappeared. This was in accordance with skin colour chroma a* measurements and the visually assessed skin erythema and scaliness scores, indicating that the superficial nasolabial skin barrier was inferior at the maximum of a nasal cold in comparison with the skin condition when volunteers were fully recovered.
Screening for acrylate/methacrylate allergy in the baseline series: our experience in Sweden and Singapore
Authors: Goon Anthony Teik‐Jin , Bruze Magnus , Zimerson Erik , Goh Chee‐Leok , Soo‐Quee Koh David , Isaksson Marléne 
Published: August 2010

Background:  No studies to specifically determine the prevalence of contact allergy to acrylates/methacrylates in patch tested populations have been published.Objectives:  To determine the prevalence of acrylate/methacrylate allergy in all patients tested to the baseline patch test series.Methods:  Five acrylate/methacrylate allergens (2‐hydroxyethyl methacrylate, methyl methacrylate, ethylene glycol dimethacrylate, triethylene glycol diacrylate, and 2‐hydroxypropyl acrylate) were included in the baseline series for at least 2 years in Malmö and Singapore.Results:  Thirty‐eight patients in total had reacted to acrylate/methacrylate allergens in the baseline series during the study period in both populations. In Malmö, there were 26 (1.4%) patients with positive patch tests to acrylate/methacrylate allergens, 14 of whom had relevant reactions. In Singapore, there were 12 (1.0%) patients with positive patch tests to acrylate/methacrylate allergens, but only 1 had relevant reactions. If we had not added acrylate/methacrylate allergens to the baseline series, we would not have patch tested 13/26 (50%) of the positive reactors in Malmö and 11/12 (92%) of the positive reactors in Singapore. The overall proportion of missed positive reactors would have been 24/38 (63%).Conclusions:  The prevalence of acrylate/methacrylate allergy in our patch tested dermatitis populations is 1.4% in Malmö and 1.0% in Singapore.
Effect of occlusal (mechanical) stimulus on bone remodelling in rat mandibular condyle
Authors: Gazit D. , Ehrlich J. , Kohen Y. , Bab I. 
Published: August 2010

Gazit D, Ehrlich J, Kohen Y, Bab I. Effect of occlusal (mechanical) stimulus on bone remodelling in rat mandibular condyle. J Oral Pathol 1987: 16: 395–398.Mechanical load influences the remodelling of skeletal tissues. In the mandibular condyle, occlusal alterations and the consequent mechanical stimulus induce changes in chondrocytes and cartilage mineralization. In the present study we quantified in the mandibular condyle the effect of occlusal interference on remodelling of the subchondral bone. Computerized histomorphometry after 5–21‐day exposure to the influence of a unilateral occlusal splint revealed an increased rate of trabecular remodelling, consisting of enhancement in osteoblast and osteoclast numbers and activities. The bone formation parameters reached their high values on Days 5 or 9 and remained stable thereafter. Bone resorption showed a gradual increase throughout the experimental period. These results further characterize the temporomandibular joint reaction to occlusal alterations. It is suggested that the present increase in bone turnover together with the known enhancement in chondrogenesis are part of a process of functional adaptation in response to mechanical stimulus.
Chemical and physical oral findings in a case of anorexia nervosa and bulimia
Authors: Jensen Ø. E. , Featherstone J. D. B. , Stege P. 
Published: August 2010

Jensen ØE, Featherstone JDB, Stege P. Chemical and physical oral findings in a case of anorexia nervosa and bulimia. J Oral Pathol 1987: 16: 399–402.The deleterious effect on enamel of continual acid challenge from regurgitation of gastric contents in bulimic subjects is well documented. No previous reports, however, have been published on chemical and physical measurements of teeth from such subjects, or on the importance of fluoride in maintaining their dentition. Teeth were obtained from a deceased anorexic and bulimic patient. Shortly before death this patient had saliva analyses and in vivo enamel biopsies at our clinic, before and after a 3‐week regimen of daily rinsing with 0.05% NaF. After 4 years of daily regurgitation there was an almost normal thickness of the enamel surfaces typically “at risk”, i.e. the palatal surfaces of the maxillary anterior teeth, with normal hardness measurements 10μm away from the outer surface. SEM micrographs showed an irregular topography, with crystalline deposits rich in calcium, phosphate and fluoride. These surfaces were more reactive to uptake of additional fluoride when given as a daily rinse, than the more protected, and, therefore, presumed unaffected surfaces. Our findings suggest that frequent and meticulous oral hygiene measures can substantially minimize the erosive effect on tooth enamel of gastric contents in bulimic patients, especially if fluoride products are used regularly.
Rheumatoid nodule in the oral mucosa. A case report with immunohistochemical study
Authors: Skov B. G. 
Published: August 2010

Skov, BG. Rheumatoid nodule in the oral mucosa. J Oral Pathol 1987: 16: 403–405.A case of rheumatoid nodule in the buccal mucosa is reported. Histologically, 3 of zones characteristic rheumatoid nodules were recognized: a central necrotic area, an intermediate zone with palisading mesenchymal cells and an outer layer composed of chronic inflammatory cells. By immunohistochemical studies, ferritin was found in some of the cells in the intermediate zone, suggesting that these cells may be of histiocytic origin. Granuloma annulare and necrobiosis lipoidica may be differential diagnoses.
Peroxidases: past and present
Authors: Paul K.G. 
Published: August 2010

Paul KG. Peroxidases: past and present. J Oral Pathol 1987: 16: 409–411.The history of peroxidases spans nearly two centuries. Our knowledge has developed from early phenomenological observations of the colored products of peroxidase‐catalyzed reactions, to our present understanding of many of the steps in the complex peroxidation reaction mechanism. Peroxidases are ubiquitous in plant and animal tissues and occur in diverse structural forms. Collectively, they are able to catalyze the hydroperoxide oxidation of many different kinds of organic and inorganic compounds. In spite of the great diversity of structures and functions, mechanisms of heme‐containing peroxidases have several common features: (i) the transfer of the oxidizing equivalents of the hydroperoxidase to the enzyme to form Compound I, (ii) the reduction of Compound I by the transfer of electrons from donor molecules, (iii) the inactivation of Compound I by excess hydroperoxide. Rate constants for these and other steps in the peroxidation mechanism, as well as redox potentials, have been reported for many peroxidases. The molecular basis for the donor specificity of peroxidases has not yet been elucidated. Today, much interest is directed towards the biological functions of peroxidases and their reaction products.
Recrudescent herpes simplex infection mimicking primary herpetic gingivostomatitis
Authors: Christie S. N. , McCaughey C. , Marley J. J. , Coyle P. V. , Scott D. A. , Lamey P.‐J. 
Published: August 2010

Christie SN, McCaughey C, Marley J J, Coyle PV, Scott DA, Lamey P‐J: Recrudescent herpes simplex infection mimicking primary herpetic gingivostomatitis.We report six cases of recrudescent intraoral herpes simplex infection clinically indistinguishable from primary herpetic gingivostomatitis. All infections occurred in healthy children or young adults. Serological analysis demonstrated herpes simplex virus (HSV)‐specific IgG at initial presentation, indicating that the infection was not a primary infection. Convalescent sera exhibited HSV‐specific IgM and a rising HSV‐specific IgG titre. These findings demonstrate that the initial clinical diagnosis of primary herpetic gingivostomatitis was erroneous and that what was actually being observed was widespread recrudescent intraoral herpes simplex infection.
Expression of heat shock proteins in squamous cell carcinoma of the tongue: an immunohistochemical study
Authors: Ito Takaaki , Kawabe Ryoichi , Kurasono Yukiko , Hara Masamichi , Kitamura Hitoshi , Fujita Kiyohide , Kanisawa Masayoshi 
Published: August 2010

Ito T, Kawabe R, Kurasono Y, Hara M, Kitamura H, Fujita K, Kanisawa M: Expression of heat shock proteins in squamous cell carcinoma of the tongue: an immunohistochemical study. J Oral Pathol Med 1998; 27: 18–22. © Munksgaard, 1998.Twenty‐four specimens of squamous cell carcinoma of the tongue were immuno‐stained for heat shock proteins (HSPs) to reveal differences in stainability among normal epithelium, dysplasia and carcinoma and to clarify the prognostic significance of HSPs in comparison with survival period, clinical stage, lymph node metastasis, histological grade, and p53 immunostaining. Normal epithelium was positively stained in the suprabasal layer for HSP60 and HSP70, but was negative for HSP27 and HSP90. Dysplastic lesions were positive for HSP27, HSP70 and HSP90, but stained variously for HSP60. In squamous cell carcinoma, the cytoplasm of suprabasal tumor cells was often positive for HSP27 and HSP90 (18/24, 17/24, respectively). Although HSP immunohistochemistry has revealed changes in HSP expression during tumorigenesis of squamous epithelium of the tongue, there was no correlation between HSP staining and survival period, stage, lymph node metastasis, histological grade or p53 immunostaining.
Experimental odontogenic cysts induced byin vitro4‐nitroquinoline 1‐oxide (4NQO) treatment of F344 rat incisor tooth germs
Authors: Miura Yoshihiro , Ozaki Hiroki S. , Li Tie‐Jun , Uemura Masanori , Kitano Motoo 
Published: August 2010

Miura Y, Ozaki HS, Li T‐J, Uemura M, Kitano M: Experimental odontogenic cysts induced byin vitro4‐nitroquinoline 1‐oxide (4NQO) treatment of F344 rat incisor tooth germs. J Oral Pathol Med 1998; 27: 53–8. © Munksgaard, 1998.This study was designed to establish an experimental animal model for elucidating the early stages of odontogenic cysts and tumors. It involves thein vitrotreatment of tooth germs with 4‐nitroquinoline 1‐oxide (4NQO) at the early bell stage and their subsequent transplantation into the kidney subcapsular space. While all tooth germ transplants of the control group not exposed to the carcinogen showed continued tooth development with no pathological lesions, 21 of 23 4NQO‐treated tooth germs developed into similar appearing keratinized cysts with or without associated tooth structures. The remaining two transplants failed to develop cysts and formed only a tooth. The present experimental procedure was effective in inducing keratinized cystic lesions that exhibit some similarities to human odontogenic keratocysts or primordial cysts.
Assessment of growth potential by MIB‐1 immunohistochemistry in ameloblastic fibroma and related lesions of the jaws compared with ameloblastic fibrosarcoma
Authors: Sano Kazuo , Yoshida Shin‐ichi , Ninomiya Hidenori , Ikeda Hisazumi , Ueno Kei , Sekine Joji , Iwamoto Hiroaki , Uehara Masataka , Inokuchi Tsugio 
Published: August 2010

Sano K, Yoshida S, Ninomiya H, Ikeda H, Ueno K, Sekine J, Iwamoto H, Uehara M, Inokuchi T: Assessment of growth potential by MIB‐1 immunohistochemistry in ameloblastic fibroma and related lesions of the jaws compared with ameloblastic fibrosarcoma. J Oral Pathol Med 1998; 27: 59–63. © Munksgaard, 1998.Specimens from two ameloblastic fibromas (including one recurrent case), two ameloblastic fibro‐odontomas, and one ameloblastic fibrosarcoma were subjected to investigation by MIB‐1 immunohistochemistry in order to elucidate the growth potential of these tumors. MIB‐1 labeling indices in the epithelial component of these tumors ranged from 2.9 to 7.5%, whereas those in the mesenchymal component ranged from 1.5 to 13.5%. Of these, labeling indices in the mesenchymal component of the recurrent ameloblastic fibroma and ameloblastic fibrosarcoma were quite high. These findings suggest that evaluation of growth potential in ameloblastic fibroma and related lesions could be of help in understanding tumor aggressiveness and in selecting appropriate surgical procedures.
Histological effects and predictive biomarkers of TPP induction chemotherapy for oral carcinoma
Authors: Hamakawa Hiroyuki , Bao Yang , Takarada Manabu , Tanioka Hiroaki 
Published: August 2010

Hamakawa H, Bao Y, Takarada M, Tanioka H: Histological effects and predictive biomarkers of TPP induction chemotherapy for oral carcinoma. J Oral Pathol Med 1998; 27: 87–94. © Munksgaard, 1998.The effects of an induction chemotherapy with THP‐adriamycin, cisplatin, and peplomycin (TPP) were studied in 32 patients with operable oral cancer. The histological evaluation according to the Shimozato‐Oboshi classification was Grade (G) IV in ten cases (31.3%), GUI in one case, and Glib in four cases. Induction of apoptosis and differentiation‐inducing effects, hyperkeratinization or bone formation, were observed in some cases. The overall clinical response rate and histological response rate were 63% and 47%, respectively. Grade III was obtained in seven metastatic lymph nodes of three patients. The expressions of PCNA, p53, and AgNORs before and after chemotherapy were studied. The prechemotherapeutic PCNA positive cell index (PI) of the highly responsive tumors (GUI, IV) was significantly lower than that of the poorly responsive tumors (G0‐Ilb) (P < 0.01). Similar results were obtained in the evaluation of p53 PI (P < 0.05), suggesting that PCNA and p53 are useful biomarkers for predicting the efficacy of TPP chemotherapy.
Immunocompetent cells in amalgam‐associated oral lichenoid contact lesions
Authors: Laine Juhani , Konttinen Yrjö T. , Beliaev Nikita , Happonen Risto‐Pekka 
Published: August 2010

ABSTRACTInflammatory cells in amalgam‐associated, oral lichenoid contact lesions (OLL) were studied in 19 patients by immunocytochemistry using monoclonal antibodies. Ten of the patients displayed allergic patch test (PT) reactions to several mercury compounds and nine were negative. The immunocytochemical quantification showed a uniform composition of the inflammatory mononuclear cells in the two study groups. The number of HLA‐D/DR‐positive dendritic cells (P<0.001) and CD1a‐positive Langerhans cells (P=0.035) was significantly lower in the PTnegative than PT‐positive patients. HLA‐D/DR expression on keratinocytes varied from negative to full thickness staining of the epithelium. HLA‐D/DR expression in the full thickness of epithelium (3) or through the basal and spinous cell layers (2) was seen in 5 of 8 PT‐positive patients, whereas none of the PT‐negative patients had this staining pattern (P=0.045). These patients also showed a good clinical response after amalgam removal. Consequently, OLL may represent a true delayed hypersensitivity reaction with a trans‐epithelial route of entrance of the metal haptens released from dental restorative materials.
Oral manifestations of HIV infection in a group of predominantly ethnic Chinese
Authors: Tsang P. C. S. , Samaranayake L. P. 
Published: August 2010

ABSTRACTA total of 32 HIV‐infected, predominantly ethnic Chinese individuals from Hong Kong were examined for oral mucosal lesions over a period of 1 year. The commonest oral lesion found was minor aphthous ulceration (27.4%), while xerostomia (17.8%), ulceration NOS (not otherwise specified; 12.3%), hairy leukoplakia (11.0%) and erythematous candidiasis (6.9%) were less frequent; Kaposi's sarcoma was notable for its absence. When the relationship between the number of oral lesions with age, risk group, medication taken, CDC staging and CD4+ count of the study group was investigated, a significantly higher number of oral lesions was associated with use of AZT, homosexuals and CDC stage IV; in contrast, a smaller number of lesions was found in those on antiparasitics and multivitamins (all P<0.05). When compared with studies from other parts of the world, the frequency of oral lesions appeared to be less common in the current study group. However, due to a lack of similar studies from the Asian region, especially in ethnic Chinese, it is not clear whether this difference could be attributed to racial, social or geographic factors.
K‐Ras gene status and expression of Ras/mitogen‐activated protein kinase (MAPK) signaling molecules in ameloblastomas
Authors: Kumamoto Hiroyuki , Takahashi Nobuhiro , Ooya Kiyoshi 
Published: August 2010

Background:  To clarify the roles of rat sarcoma (Ras)/mitogen‐activated protein kinase (MAPK) signaling pathway in oncogenesis and cytodifferentiation of odontogenic tumors, K‐Ras gene status and expression of Ras, Raf1, MAPK/extracellular signal‐regulated kinase (ERK) kinase (MEK)1, and ERK1/2 proteins were analyzed in ameloblastomas as well as in tooth germs.Methods:  Paraffin sections of 10 tooth germs and 46 benign and 6 malignant ameloblastomas were examined immunohistochemically for the expression of K‐Ras, Raf1, MEK1, and ERK1/2. Frozen tissue samples of 22 benign ameloblastomas and 1 malignant (metastasizing) ameloblastoma were analyzed by direct DNA sequencing to detect K‐Ras gene alteration.Results:  Immunohistochemical reactivity for K‐Ras, Raf1, MEK1, and ERK1/2 was detected in both normal and neoplastic odontogenic epithelium, and these molecules were reactive chiefly with odontogenic epithelial cells neighboring the basement membrane. Plexiform ameloblastomas showed slightly stronger expression of these Ras/MAPK signaling molecules than follicular ameloblastomas. Keratinizing cells and granular cells showed decreased reactivity for the signaling molecules. Basal cell ameloblastomas showed slightly stronger reactivity for the signaling molecules than did the other subtypes. K‐Ras immunoreactivity in malignant ameloblastomas was lower than that in dental lamina of tooth germs. Direct DNA sequencing showed a GGT to GCT point mutation at codon 12 of K‐Ras gene in one ameloblastoma.Conclusion:  Expression of K‐Ras, Raf1, MEK1, and ERK1/2 in tooth germs and ameloblastomas suggests that Ras/MAPK signaling pathway functions to regulate cell proliferation and differentiation in both normal and neoplastic odontogenic epithelium. K‐Ras gene status implied that K‐Ras mutations might play a minor role in oncogenesis of odontogenic epithelium.
Correlation between functional genotypes in the matrix metalloproteinases‐1 promoter and risk of oral squamous cell carcinomas
Authors: Lin Shu‐Chun , Chung Ming‐Yi , Huang Jen‐Wu , Shieh Tzong‐Ming , Liu Chung‐Ji , Chang Kuo‐Wei 
Published: August 2010

Background:  Oral squamous cell carcinoma (OSCC) and oral submucous fibrosis (OSF), which are highly associated with areca use, are prevalent in most Asian countries. Matrix metalloproteinases (MMPs) are superfamily of metal‐dependent proteolytic enzymes, mediating the degradation of extracellular matrix. Insertion/deletion (−1607 2G→1G) polymorphism has been described in the promoter region of the human matrix metalloproteinases‐1 (MMP‐1) genes, which cause an alteration in the transcriptional activity. This genotype is associated with risks of cancer genesis and metastasis. In this paper, we studied the relationship between such genotype and areca‐associated oral diseases.Methods:  Genomic DNA from the blood of OSCC (n = 121), OSF (n = 58) cases and controls (n = 147) were amplified by polymerase chain reaction (PCR)‐based genotyping. The OSCC were further grouped into buccal squamous cell carcinoma (BSCC) and non‐buccal suqmaous cell carcinoma (NBSCC), in accord with the site of involvement. The significance of the differences was assessed by Fisher's exact test.Results:  The 2G genotype in MMP‐1 promoter was observed with a higher frequency in both OSCC (0.69, P = 0.06) and NBSCC (0.76, P = 0.03) cases compared with controls (0.63), with an odds ratio of 2.17 and 4.58, respectively. This genotype was not related to the risk of OSF. No other clinicopathologic parameter was associated with the genotypes in OSCC cases.Conclusion:  The results showed that 2G genotype in MMP‐1 promoter was associated with the risk of OSCC.
Quantitative analysis of argyrophilic nucleolar organizer regions and epidermal growth factor receptor in ameloblastomas
Authors: Payeras M. R. , Sant'Ana Filho M. , Lauxen I. S. , Barbachan J. J. D. 
Published: August 2010

Objective:  The aim of this study was to evaluate the proliferation activity by means of the quantification of the argyrophilic nucleolar organizer regions (AgNORs) and the patterns of expression of the epidermal growth factor receptor (EGFR) in ameloblastomas.Method:  The methods of evaluation included the H/E stain for the morphologic analysis, the silver impregnation technique for quantification of the AgNORs and the immunohistochemical stain with anti‐EGFR antibody in 11 cases of ameloblastoma.Results:  The results did not show a significant statistical difference as per quantification of the AgNORs. The expression of the EGFR on the epithelial islands of ameloblastoma was not uniform, and the location of the expression was also variable. The predominant expression was that of cytoplasm and the islands with an expression of membrane only were rare and generally smaller in size.Conclusion:  The tumor presents an irregular growth. Smaller islands are associated with a higher proliferation activity and therefore could be responsible for tumor infiltration.