bluuebunny/crossref_metadata_2025_split huggingface dataset:
EBP50 is involved in the regulation of vascular smooth muscle cell migration and cytokinesis
Authors: Baeyens Nicolas , de Meester Carole , Yerna Xavier , Morel Nicole 
Published: May 2011

AbstractEzrin, Radixin, Moesin binding phosphoprotein 50 (EBP50) is a scaffold protein that possesses two PDZ interacting domains. We have shown that, in isolated artery stimulated with noradrenaline, EBP50 interacts with several elements of the cytoskeleton. However, the contribution of EBP50 to the organization of the cytoskeleton is unknown. We have used primary cultured vascular smooth muscle cells to investigate the involvement of EBP50 in the regulation of cell architecture, motility and cell cycle, and to identify its target proteins and subsequent action mechanism. The results showed that depletion of EBP50 by siRNA transfection induced changes in cell architecture and increased cell migration. The same phenotype was induced by inhibition of myosin IIa and this effect was not additive in cells depleted for EBP50. Moreover, a larger proportion of binucleated cells was observed after EBP50 depletion, indicating a defect in cytokinesis. The identification, after co‐immunoprecipitation, of a direct interaction of EBP50 with both tubulin and myosin IIa suggested that EBP50 could regulate cell migration and cytokinesis by linking myosin IIa fibers and microtubule network. Indeed, depletion of EBP50 also dismantled myosin IIa fibers and induced the formation of stable microtubules in lamellae expansions and Rac1 activation. This signaling cascade leads to the formation of lamellipodia, trailing tails and decrease of focal adhesion formation, triggering cell migration. J. Cell. Biochem. 112: 2574–2584, 2011. © 2011 Wiley‐Liss, Inc.
The structure, properties, and nature of HArF‐HOX (X = F, Cl, Br) complex: An ab initio study and an unusual short hydrogen bond
Authors: Li Qing‐Zhong , Zhao Jun‐Ling , Jing Bo , Li Ran , Li Wen‐Zuo , Cheng Jian‐Bo 
Published: May 2011

AbstractThe structure and properties (geometric, energetic, electronic, spectroscopic, and thermodynamic properties) of HArF‐HOX (X = F, Cl, Br) complex have been investigated at the MP2/aug‐cc‐pVTZ level. Three types of complexes are formed through a hydrogen bond or a halogen bond. The HArF‐HOX complex is the most stable, followed by the FArH‐OHX complex, and the HArF‐XOH complex is the most unstable. The binding distance in FArH‐OHX complex is very short (1.1–1.7 Å) and is smaller than that in HArF‐HOX complex. However, the interaction strength in the former is weaker than that in the latter. Thus, an unusual short hydrogen bond is present in FArH‐OHX complex. The associated H‐Ar bond exhibits a red shift, whereas the distant one gives a blue shift. A similar result is also found for the OH and OX bonds. The isotropic chemical shift is negative for the associated hydrogen atom but is positive for the associated halogen atom. However, a reverse result is found for the anisotropic chemical shift. The analyses of natural bond orbital and atoms in molecules have been performed for these complexes to understand the nature and properties of hydrogen and halogen bonds. © 2011 Wiley Periodicals, Inc. J Comput Chem, 2011
An expeditious one‐pot synthesis of substituted phenylazetidin‐2‐ones in the presence of zeolite
Authors: Pagadala Ramakanth , Meshram Jyotsna S. , Chopde Himani N. , Jetti Venkateshwarlu , Udayini V. 
Published: May 2011

AbstractIn this study, one‐pot rapid and efficient series of phenylazetidin‐2‐ones were synthesized from N,N‐dimethylaminobenzaldehyde, different substituted aromatic amines and phenylacetyl chloride in the presence of zeolite catalyst under microwave irradiation. We also reported schiff bases (1a–j) by classical and conventional microwave technique. The titled compounds are evaluated for their antimicrobial properties. The activities are due to CO, CN, linkages in 2‐azetidinones. All the compounds have shown comparable antibacterial activities. J. Heterocyclic Chem., (2011).
One‐step ring‐closure procedure for 4,5‐dihydro‐1,3‐thiazino[5,4‐b]indole derivatives with Lawesson's reagent. The fifth dihydro‐1,3‐thiazino[b]indole isomer
Authors: Csomós Péter , Fodor Lajos , Bernáth Gábor , Csámpai Antal , Sohár Pál 
Published: May 2011

AbstractWe report a convenient approach for the synthesis of a new ring system: 4,5‐dihydro‐1,3‐thiazino[5,4‐b]indoles. The procedure involves the use of Lawesson's reagent in the presence of silica to achieve the one‐step ring‐closure reactions of 2‐benzoylamino‐3‐hydroxymethylindole intermediates to furnish 4,5‐dihydro‐2‐aryl‐1,3‐thiazino[5,4‐b]indoles. 2‐Phenylimino‐1,3‐thiazino[5,4‐b]indoles were obtained via the corresponding 3‐phenylthiourea‐2‐carboxylic acid ester derivatives by chemoselective reduction of the ester group, followed by ring closure under acidic conditions. The structures of the novel products were elucidated by IR, 1H‐NMR, and 13C‐NMR spectroscopy, including 2D‐HMQC, 2D‐HMBC, and DEPT measurements. J. Heterocyclic Chem., (2011).
Regioselective synthesis and characterization of new 3‐aryl‐7‐trifluoromethyl‐[1,2,4]triazolo[4,3–a]pyrimidines
Authors: Zanatta Nilo , Amaral Simone S. , dos Santos Josiane M. , Fernandes Liana da S. , Bonacorso Helio G. , Martins Marcos A. P. 
Published: May 2011

AbstractThe regioselective synthesis and characterization of a new series of 3‐aryl‐7‐trifluoromethyl[1,2,4]triazolo[4,3–a]pyrimidines from the oxidative heterocyclization of 2‐(N′‐benzylidenehydrazino)‐4‐trifluoromethylpyrimidines with copper dichloride is described. J. Heterocyclic Chem., (2011).
One‐pot‐one‐step, microwave‐assisted Fischer indole synthesis
Authors: Creencia Evelyn Cuevas , Tsukamoto Masayuki , Horaguchi Takaaki 
Published: May 2011

AbstractThe Fischer indole synthesis was carried out using microwaves instead of conventional heating procedures. When the mixture of phenylhydrazine, cyclohexanone and zinc chloride was irradiated at 600 W for 3 min, 76% of 1,2,3,4‐tetrahydrocarbazole was obtained. However, when zinc chloride was replaced with p‐toluenesulfonic acid (p‐TSA), the reaction yielded 91% of 1,2,3,4‐tetrahydrocarbazole. Thus, a series of indoles were prepared using microwaves in the presence of p‐TSA catalyst. J. Heterocyclic Chem., (2011).
Quantitative assessment of cardiac output and left ventricular function by noninvasive phase‐contrast and cine MRI: Validation study with invasive pressure‐volume loop analysis in a swine model
Authors: Lin Hung‐Yu , Freed Darren , Lee Trevor W.R. , Arora Rakesh C. , Ali Ayyaz , Almoustadi Waiel , Xiang Bo , Wang Fei , Large Stephen , King Scott B. , Tomanek Boguslaw , Tian Ganghong 
Published: May 2011

AbstractPurpose:To validate noninvasive cardiac output measurements of phase‐contrast magnetic resonance imaging (PC‐MRI) and cine MRI using an invasive pressure‐volume (PV) loop technique on a swine model.Materials and Methods:We compared three methods for evaluating cardiac function at rest and under pharmaceutical low‐dose inotropic infusion conditions: 1) phase‐contrast MRI, 2) cine MRI, and 3) PV loop relationship. These measurements were made in 14 domestic pigs under rest conditions. Identical MRI acquisitions and PV loop analysis were performed on six pigs from the same group that received an infusion of dobutamine 2.5 μg/kg/min. Cardiac outputs from all measurements were analyzed and compared using linear regression and Bland–Altman analysis.Results:Noninvasive PC‐MRI and cine MRI did not show any significant differences compared to an invasive PV loop technique for measurement of cardiac output under both rest (PC‐MRI, cine MRI, and PV loop, 3.17 ± 0.45, 3.18 ± 0.61, 3.45 ± 0.41 L/min, respectively) and pharmaceutical low‐dose inotropic infusion conditions (PC‐MRI, cine MRI, and PV loop, 4.78 ± 0.53, 4.7 ± 0.6, 4.96 ± 0.48 L/min, respectively). Statistical analysis showed good agreement of cardiac output measurements at rest (R2 = 0.83) and under low‐dose inotropic infusion conditions (R2 = 0.74) using PC‐MRI and PV loop techniques. Cardiac output measurement using cine MRI and PV loop techniques also showed good agreement at rest (R2 = 0.85) and under low‐dose inotropic infusion conditions (R2 = 0.76). Furthermore, cardiac outputs determined with the three modalities showed good agreement over a wide range of heart rates (90–180 bpm).Conclusion:MRI can provide a reliable, noninvasive measurement of cardiac output that can be carried out without the complications that are inherent with current invasive procedures. J. Magn. Reson. Imaging 2011;. © 2011 Wiley‐Liss, Inc.
A highly sensitive LC‐MS/MS method capable of simultaneously quantitating celiprolol and atenolol in human plasma for a cassette cold‐microdosing study
Authors: Minamide Yoshiyuki , Osawa Yukio , Nishida Hiroshi , Igarashi Harue , Kudoh Shinobu 
Published: May 2011

AbstractA highly sensitive simultaneous quantitative method for a cassette cold‐microdosing study on celiprolol and atenolol was developed with liquid chromatography–tandem mass spectrometry. The method utilizes a combination of solid‐phase extraction (SPE) with strong cation exchange (SCX) cartridge columns and reversed‐phase chromatography with an ODS analytical column. SCX‐SPE cartridge columns (100 mg sorbent) were used for a selective extraction of celiprolol, atenolol and metoprolol (internal standard) from 500 μL of human plasma samples. Turbo‐ion spray at positive mode was employed for the ionization of the drug compounds. Quantitation was performed on a triple quadrupole mass spectrometer by selected reaction monitoring with the transitions of m/z 380 to m/z 251 for celiprolol and m/z 267 to m/z 145 for atenolol. Separation of analytes was achieved on an ODS column (100 mm length×2.1 mm id, 3 μm) by a gradient elution with 10 mM formic acid and methanol by varying their proportion at a flow rate of 0.2 mL/min. The method was validated in the range of 1–250 pg/mL for celiprolol and 2.5–250 pg/mL for atenolol and was successfully applied to the elucidation of pharmacokinetic profiling in a cold cassette microdosing study of the β‐blockers.
Rapid analysis of aldehydes by simultaneous microextraction and derivatization followed by GC‐MS
Authors: Ye Qing , Zheng Dagui , Liu Linhai , Hong Liming 
Published: May 2011

AbstractUltrasound‐assisted dispersive liquid–liquid microextraction (UDLLME) and simultaneous derivatization followed by GC‐MS was developed for the analysis of four aldehydes including acetaldehyde (ACE), propionaldehyde (PRO), butyraldehyde (BUT) and valeraldehyde (VAL) in water samples. In the proposed method, the aldehydes were derivatized with O‐2,3,4,5,6‐(pentafluorobenzyl)hydroxylamine (PFBHA) and extracted by UDLLME in aqueous solution simultaneously; finally, the derivatives were analyzed by GC‐MS. The experimental parameters were investigated and the method validations were studied. The optimal conditions were: aqueous sample of 5 mL, PFBHA of 50 μL, 1.0 mL ethanol (disperser solvent) containing 20 μL chlorobenzene (extraction solvent), ultrasound time of 2 min and centrifuging time of 3 min at 6000 rpm. The proposed method provided satisfactory precision (RSD 1.8–10.2%), wide linear range (0.8–160 μg/L), good linearity (R2 0.9983–0.9993), good relative recovery (85–105%) and low limit of detection (0.16–0.23 μg/L). The proposed method was successfully applied for the analysis of aldehydes in water samples. The experimental results showed that the proposed method was a very simple, rapid, low‐cost, sensitive and efficient analytical method for the determination of trace amount of aldehydes in water samples.
A novel RP‐HPLC method for simultaneous determination of potassium sorbate and sodium benzoate in soft drinks using C18‐bonded monolithic silica column
Authors: Can Nafiz O. , Arli Goksel , Lafci Yigit 
Published: May 2011

AbstractPotassium sorbate and sodium benzoate are food additives that are generally employed for prevention of food spoilage originating from bacteria, molds or yeasts. Although these compounds were generally recognized as safe due to their low risk of acute and chronic toxicity, they have limitations of usage to protect human health. Development and validation of a novel RP‐HPLC method, in which a C18‐bonded monolithic silica column was used as stationary phase to assay these compounds, is described for the first time. Aliquots of 10 μL of samples were injected into chromatograph and eluted using phosphate buffer (0.025 M, pH 2.0)–water–acetonitrile (50:45:5, v/v/v) solution, which was pumped at the rate of 3.0 mL/min. To sharpen the peaks, 10 mM octylamine was added to the mobile phase. Potassium sorbate and sodium benzoate were detected at about 12th and 14th min, respectively, and quantified at 230 nm using photodiode array detector. A total of 41 samples were prepared by simply filtering through 0.45 μm filters after sonication, and injected into the system without any pre‐treatment steps. Applicability of the method was demonstrated by performing total procedure on samples of different brands and types, and their compliance to official regulations was assessed.
Involvement of the transcription factor twist in phenotype alteration through epithelial–mesenchymal transition in lung cancer cells
Authors: Nakashima Harunori , Hashimoto Naozumi , Aoyama Daisuke , Kohnoh Takashi , Sakamoto Koji , Kusunose Masaaki , Imaizumi Kazuyoshi , Takeyama Yoshihiro , Sato Mitsuo , Kawabe Tsutomu , Hasegawa Yoshinori 
Published: May 2011

AbstractEpithelial–mesenchymal transition (EMT), which involves the persistent loss of epithelial markers and expression of mesenchymal markers, is assumed to have a critical role in not only tissue development during embryogenesis but also central mechanisms that enhance the invasive and metastatic ability of cancer cells. Twist has been identified to play an essential role in EMT‐mediated tumor invasion and metastasis. Although recent studies suggest that twist expression levels in tissue specimens of lung cancer might be associated with prognosis, the expression of twist in lung cancer cells itself and its effect have not been fully evaluated. Here, we evaluated twist expression and its effect on phenotype alteration in lung cancer cell lines. Twist expression varied among human lung cancer cell lines. The lung cancer cell lines with high twist expression also tended to show a high vimentin/E‐cadherin ratio, which was supported by a migration assay, in which high twist expression gave rise to high cell motility. Furthermore, in comparison to control cells, the lung cancer cells with ectopic expression of twist showed a significant phenotype alteration through EMT and an increasing ability to migrate in vitro, in part, due to a tenfold increase in matrix metalloproteinases activity and almost a 60% increase in modulation of focal adhesion kinase activity, although a contribution of microRNA appeared unlikely in our study. Our present analysis of twist expression in lung cancer provide clues to comprehensive understanding of the mechanisms, by which metastasis often develops in lung cancer. © 2011 Wiley Periodicals, Inc.
Quality of life in patients with treated and clinically stable Wilson's disease
Authors: Svetel Marina , Pekmezović Tatjana , Tomić Aleksandra , Kresojević Nikola , Potrebić Aleksandra , Ješić Rada , Kostić Vladimir S. 
Published: May 2011

AbstractHealth‐related quality of life (HRQoL) in Wilson's disease (WD) has not been extensively studied. Therefore, the purpose of this cross‐sectional study was to identify clinical and demographic factors influencing HRQoL in 60 treated, clinically stable patients with WD using a generic questionnaire, the Medical Outcomes Study Short‐Form 36‐Item Health Survey (SF‐36). The level of disability and grading of WD multisystemic manifestations were assessed by the Global Assessment Scale for WD (GAS for WD). The Mini Mental State Examination (MMSE) and the 21‐item Hamilton Depression Rating Scale (HDRS) scoring were also applied by the same trained interviewers. Lower scores on the SF‐36 domains were found in patients with neurological compared with those with a predominantly hepatic form of WD. The HRQoL of patients with WD and psychiatric symptoms was also lower than that of those without them. Finally, significant inverse correlations were obtained between the various SF‐36 domains and all the following: period of latency from the first symptoms/signs appearance and treatment initiation, MMSE and HDRS scores, and different domains of the GAS for WD. © 2011 Movement Disorder Society
Phenethyl isothiocyanate sensitizes human cervical cancer cells to apoptosis induced by cisplatin
Authors: Wang Xiantao , Govind Sudha , Sajankila Shyama P. , Mi Lixin , Roy Rabindra , Chung Fung‐Lung 
Published: May 2011

AbstractScope: Naturally‐occurring chemopreventive agent phenethyl isothiocyanate (PEITC), derived primarily from watercress, has been shown to inhibit cell growth and induce apoptosis in cancer cells. In this study, we examined the potential of PEITC in enhancing cisplatin‐induced apoptosis in cervical cancer cells and its mechanisms.Methods and results: HeLa cells were exposed to PEITC, cisplatin or both. Pretreatment of cells with PEITC strongly enhanced cisplatin‐induced cytotoxicity. PEITC activated the mitogen‐activated protein kinases, including c‐Jun N‐terminal kinase (JNK), extracellular signal‐related kinase (ERK), and p38. Caspase‐3 activity assay demonstrated that the synergistic induction of apoptosis was significantly attenuated by MEK1/2 inhibitor U0126, but not by JNK or p38 inhibitor, suggesting that ERK activation is responsible for the synergistic effect. We found that NF‐κB signaling pathway is not involved in the synergistic effect. Sulforaphane and benzyl isothiocyanate, two other members of the isothiocyanate family, also sensitize HeLa cells to apoptosis induced by cisplatin. Furthermore, we found that the synergistic effect was also observed in cervical cancer C33A and breast cancer MCF‐7 cells but not in normal mammary epithelial MCF‐10A cells. Finally, we demonstrated that Noxa induction was associated with apoptosis induced by PEITC plus cisplatin.Conclusion: Taken together, this study shows that PEITC can sensitize cancer cells to apoptosis induced by cisplatin and this effect is mediated through ERK activation, suggesting the potential of PEITC to be used as an adjuvant with cisplatin in combination therapeutic treatments.
Vertical handover (VHO) framework for future collaborative wireless networks
Authors: Dimitriou Nikos , Sarakis Lambros , Loukatos Dimitrios , Kormentzas George , Skianis Charalambos 
Published: May 2011

SUMMARYThe paper focuses on the concept of the vertical handover (VHO) among collaborative wireless networks, which is the necessary driver for seamless mobility and thus for the ability of the user to be always best connected. The paper discusses the required enabling architecture for distributed VHO decision making that is based on the principles of the IEEE 1900.4 standard and on the key media‐independent signaling procedure related to the IEEE 802.21 standard. The proposed framework combines the ‘lower‐layer’ and ‘higher layer’ intelligence in a unified and media‐independent way, to drive handover decisions based on rich terminal and network context information. The paper provides a detailed description of the VHO framework and of the key interactions between its key functional blocks. Additionally, the paper includes a series of validation results corresponding to an implementation of the presented framework. Furthermore, the paper includes a discussion comparing the capabilities offered by the proposed framework with those proposed by other similar proposals, which shows the potential benefits and tradeoffs of the introduced concepts. Copyright © 2011 John Wiley & Sons, Ltd.
NASCA‐HMBC, a New NMR Methodology for the Resolution of Severely Overlapping Signals: Application to the Study of Agathisflavone
Authors: Bayiha Ba Njock Gaétan , Bartholomeusz Trixie Ann , Foroozandeh Mohammadali , Pegnyemb Dieudonné Emmanuel , Christen Philippe , Jeannerat Damien 
Published: May 2011

ABSTRACTIntroductionStandard NMR 2D heteronuclear HMBC spectra have a low resolution in the indirect carbon dimension, making it very difficult to assign signals to individual carbons when their chemical shifts are < 0.3 ppm apart.ObjectiveTo establish spectral aliasing for HMBC experiments to improve the resolution in the carbon dimension without increasing the total experimental time and avoiding ambiguities in the observed chemical shifts.MethodologyThe NASCA‐HMBC (Non‐ambiguous Assignment by Superposition of Coupled Aliased HMBC) methodology combines a pair of HMBC spectra recorded with slightly different carbon windows to provide typically one order of magnitude increase in the resolution and unambiguous chemical shifts.ResultsThe application of this methodology to a biflavonoid found in Ouratea gilgiana resulted in spectra with a sufficiently high resolution to make the assignment straightforward and report, for the first time, the full assignment of agathisflavone.ConclusionThe methodology should find many applications in dimeric and oligomeric compounds such as peptides, carbohydrates, polyketides and other cases where signal clustering is expected. Copyright © 2011 John Wiley & Sons, Ltd.
Synthesis, structure and properties of polyimide–silica hybrid composites based on biphenyltetracarboxylic dianhydride
Authors: Jena Kishore K , Raju Kothapalli VSN , Rout Tapan K 
Published: May 2011

AbstractA simple and efficient way of synthesizing nanocomposite films using a dispersion technique is reported, with the resulting films having improved mechanical and thermal properties. Nano‐SiO2 was used in a biphenyltetracarboxylic dianhydride‐based poly(amic acid) precursor and found to be dispersed up to 7 wt% without any additives. The composites were cast to make 10 µm solid films to establish structure and property relationships between liquid and solid film. The structures of the liquid composite materials were studied using NMR and Fourier transform infrared spectroscopy. Solid film properties such as tensile strength, contact angles and thermal behaviour were evaluated for comparison. The properties of the composite films were found to be enhanced compared to polyimide film itself. Atomic force microscopy and macroscale mechanical measurements showed that composite films with more dipolar bonding interactions have higher elastic moduli and are more deformable. They yield higher adhesion energies, and therefore composite coatings offer greater adhesion. There was a limitation in the film formation beyond 5 wt% of silica. Copyright © 2011 Society of Chemical Industry
Synthesis of pyrene‐capped polystyrene for dispersion of pristine single‐walled carbon nanotubes
Authors: Yan Yehai , Yang Shaobei , Cui Jian , Jakisch Lothar , Pötschke Petra , Voit Brigitte 
Published: May 2011

AbstractPyrene‐capped polystyrene (PyPS) with various molecular weights (M̄n) was synthesized through the anionic polymerization method and characterized using UV, Fourier transform infrared and NMR spectroscopy and gel permeation chromatography. The polymers were then used for non‐covalent functionalization of pristine single‐walled carbon nanotubes (SWNTs). The functionalization efficiency was assessed by measuring the SWNT dispersibility in chloroform. In the presence of PyPS, the dispersibility can be as high as 372.5 mg L−1, and the dispersions containing more than 1.25 mg mL−1 of PyPS are very stable with no solid deposits observed after being centrifuged at 5000 rpm for 15 min. Once the PyPS concentration is converted to the molar concentration of the pyrene unit and the dispersibility redefined as nanotube content per molar pyrene unit, the renewed dispersibility is found to be independent of M̄n of PyPS within the detected M̄n range. For a certain PyPS polymer, however, both nanotube dispersibility and dispersion stability are strongly dependent on the PyPS concentration. These results suggest that PyPS may be used as an excellent dispersant for subsequent preparation of polystyrene/SWNT composites. Copyright © 2011 Society of Chemical Industry