bluuebunny/crossref_metadata_2025_split huggingface dataset:
Associations of histone deacetylase‐2 and histone deacetylase‐3 genes with schizophrenia in a Chinese population
Authors: Han Hongzhi , Yu Yaqin , Shi Jieping , Yao Yan , Li Wenjun , Kong Ning , Wu Yanhua , Wang Chong , Wang Shibin , Meng Xiangfei , Kou Changgui 
Published: July 2012

AbstractIntroductionTo explore the association between histone deacetylase‐2 (HDAC2) and histone deacetylase‐3 (HDAC3) gene polymorphisms and schizophrenia.MethodsA total of 208 family trios consisting of fathers, mothers and affected offspring with schizophrenia were recruited as our subjects. Four tag SNPs on HDAC2 (rs10499080, rs6568819, rs2499618 and rs13204445) and two tag SNPs on HDAC3 (rs11741808, rs2530223) genes were selected. The Mass ARRAY Assay Design software (Sequenom) was used to design amplification and allele specific extension primers. The Hardy‐Weinberg equilibrium (HWE) for genotypic distributions was tested using the chi‐square goodness‐of‐fit test. Allelic association for a single tag SNP was analyzed by using family‐based association tests including the haplotype‐based haplotype relative risk (HHRR) test and the transmission disequilibrium test (TDT).ResultsThe genotypic distributions of HDAC2 SNPs rs6568819, rs2499618 and rs13204445 and HDAC3 SNPs rs11741808 and rs2530223 were all in Hardy‐Weinberg equilibrium (P > 0.05). HHRR analysis revealed no associations between the SNPs and schizophrenia (P > 0.05). In addition, the TDT did not show any significant associations between HDAC2 and HDAC3 SNPs and schizophrenia (P > 0.05).DiscussionHDAC2 and HDAC3 might not be associated with schizophrenia in the Chinese population.
Lipid profile is associated with the incidence of cognitive dysfunction in viral cirrhotic patients: A data‐mining analysis
Authors: Taniguchi Eitaro , Kawaguchi Takumi , Sakata Masahiro , Itou Minoru , Oriishi Tetsuharu , Sata Michio 
Published: July 2012

Aim:  Cognitive dysfunction (CD) is frequently observed in cirrhotic patients. However, the biochemical profiles associated with CD remain unclear. We investigated the biochemical profiles associated with the incidence of CD in cirrhotic patients by using multivariate analyses, including a decision‐tree algorithm.Methods:  In this study, 27 viral cirrhotic patients were enrolled. All subjects underwent neuropsychiatric tests; two or more abnormal results were defined as CD. A logistic regression model was used for multivariate stepwise analysis. A decision‐tree algorithm was constructed, and the categorical differences based on the decision‐tree model were analyzed by χ2‐tests.Results:  Multivariate stepwise analysis showed the levels of total bilirubin, triglycerides and free fatty acids (FFA) as independent bioparameters associated with the incidence of CD in cirrhotic patients. The decision‐tree algorithm showed that among patients with FFA of 514 mEq/L or more, 77.8% had CD. Meanwhile, among patients with FFA of less than 514 mEq/L and triglycerides of 106 mg/dL or more, 20.0% had CD. The sensitivity, specificity and accuracy for the incidence of CD using the lipid profile (FFA >514 mEq/L or triglycerides <106 mg/dL) were 85.7% (12/14), 61.5% (8/13) and 74.1% (20/27), respectively.Conclusion:  The levels of total bilirubin, FFA and triglycerides are independently associated with the incidence of CD in cirrhotic patients. In addition, a decision‐tree algorithm revealed that FFA of more than 514 mEq/L or triglycerides of less than 106 mg/dL is a profile associated with the incidence of CD. Thus, this lipid profile could be a possible screening bioparameter for CD in cirrhotic patients.
Museums of Malawi Case Study: Tackling HIV/AIDS by Creating Awareness of Rights and Cultural Practices
Authors: Gondwe Mike 
Published: July 2012

Abstract  The HIV/AIDS epidemic has greatly impacted the lives of many Malawians. In 2007, one million people in a population of 13 million were infected with the HIV virus. There are 91,000 cases of children living with HIV and over 550,000 orphans who lost their parents to this disease (UNAIDS 2008 Report on Global AIDS Epidemic). At the Museums of Malawi, many staff have died of HIV/AIDS‐related illnesses. The gravity of this matter prompted the Museums of Malawi to join hands with other stakeholders in a concerted effort to address the HIV/AIDS pandemic. The Museums of Malawi has found that the most significant reasons for HIV transmission are specific cultural beliefs and practices that abuse people’s rights, and that are as dangerous as the disease itself. The museum created activities that look at these beliefs in order to achieve behavioral change among youth and adults during the program.
Physical phantom studies of helical cone‐beam CT with exact reconstruction
Authors: Tan Jun , Li H. Harold , Klein Eric , Li Hua , Parikh Parag , Yang Deshan 
Published: July 2012

Purpose:Onboard cone‐beam computed tomography (CBCT) connected to radiotherapy linear accelerators suffers CT number consistency and uniformity problems in addition to limited longitudinal coverage. Such problems have prevented CBCT from being fully utilized in many quantitative applications including tumor response evaluation and daily radiation dose computation. This paper presents a feasibility study on the helical CBCT scan with exact reconstruction that could be a potential solution.Methods:A Varian TrueBeam treatment machine was programmed in the research mode to accomplish helical scans that required synchronized gantry circular rotation and couch table linear motion. Two physical phantoms were scanned in both 360° and 720° helical trajectories. A Katsevich exact reconstruction algorithm was implemented and tested with digital phantom simulations. It was further optimized to account for mechanical instabilities of both gantry rotation and couch table motion from the physical phantom measurements. Preprocessing was employed to correct photon scattering, beam hardening, and bowtie filtration. The reconstructed images were compared to those reconstructed from the FDK approximate reconstruction algorithm using the same phantom projections. Comparisons have also been made with the clinical circular CBCT images and the diagnostic helical CT images of the same physical phantoms.Results:Satisfactory reconstruction results were obtained for the Katsevich algorithm in digital phantom study. Physical phantom results demonstrated that a 360° helical scan could provide up to 19 cm longitudinal coverage, which could be increased to 54 cm with a 720° helical scan. Image spatial resolution and soft tissue contrast were sufficient. The Q‐value, which combined the spatial frequency response (modulation transfer function) and the image noise, was calculated, and suggested that the Katsevich algorithm was superior to the FDK algorithm.Conclusions:A helical CBCT scan is useful to extend the longitudinal coverage. The Katsevich exact reconstruction algorithm could provide additional advantages in image qualities over the traditional FDK approximate algorithm. The combination of helical CBCT scan with exact reconstruction was proved feasible and would render CBCT more useful in image‐guided radiation therapy.
Migration of the Swine Influenza Virus δ-Cluster Hemagglutinin N-Linked Glycosylation Site from N142 to N144 Results in Loss of Antibody Cross-Reactivity
Authors: Hause Ben M. , Stine Douglas L. , Sheng Zizhang , Wang Zhao , Chakravarty Suvobrata , Simonson Randy R. , Li Feng 
Published: July 2012

ABSTRACT
Routine antigenic characterization of swine influenza virus isolates in a high-throughput serum neutralization (HTSN) assay found that approximately 20% of isolates were not neutralized by a panel of reference antisera. Genetic analysis revealed that nearly all of the neutralization-resistant isolates possessed a seasonal human-lineage hemagglutinin (HA; δ cluster). Subsequent sequencing analysis of full-length HA identified a conserved N144 residue present only in neutralization-resistant strains. N144 lies in a predicted N-linked glycosylation consensus sequence, i.e., N-X-S/T (where X is any amino acid except for proline). Interestingly, neutralization-sensitive viruses all had predicted N-linked glycosylation sites at N137 or N142, with threonine (T) occupying position 144 of HA. Consistent with the HTSN assay, hemagglutination inhibition (HI) and serum neutralization (SN) assays demonstrated that migration of the potential N-linked glycosylation site from N137 or N142 to N144 resulted in a >8-fold decrease in titers. These results were further confirmed in a reverse genetics system where syngeneic viruses varying only by predicted N-glycosylation sites at either N142 or N144 exhibited distinct antigenic characteristics like those observed in field isolates. Molecular modeling of the hemagglutinin protein containing N142 or N144 in complex with a neutralizing antibody suggested that N144-induced potential glycosylation may sterically hinder access of antibodies to the hemagglutinin head domain, allowing viruses to escape neutralization. Since N-linked glycosylation at these sites has been implicated in genetic and antigenic evolution of human influenza A viruses, we conclude that the relocation of the hemagglutinin N-linked glycosylation site from N142 to N144 renders swine influenza virus δ-cluster viruses resistant to antibody-mediated neutralization.
Draft Genome Sequence of Herbaspirillum lusitanum P6-12, an Endophyte Isolated from Root Nodules of Phaseolus vulgaris
Authors: Weiss Vinícius Almir , Faoro Helisson , Tadra-Sfeir Michelle Zibbetti , Raittz Roberto Tadeu , de Souza Emanuel Maltempi , Monteiro Rose Adele , Cardoso Rodrigo Luis Alves , Wassem Roseli , Chubatsu Leda Satie , Huergo Luciano Fernandes , Müller-Santos Marcelo , Steffens Maria Berenice Reynaud , Rigo Liu Un , Pedrosa Fábio de Oliveira , Cruz Leonardo Magalhães 
Published: July 2012

ABSTRACT

Herbaspirillum lusitanum
strain P6-12 (DSM 17154) is, so far, the only species of
Herbaspirillum
isolated from plant root nodules. Here we report a draft genome sequence of this organism.
Genome Sequence of Mycobacterium massiliense M18, Isolated from a Lymph Node Biopsy Specimen
Authors: Ngeow Yun Fong , Wong Yan Ling , Tan Joon Liang , Arumugam Ramitha , Wong Guat Jah , Ong Chia Sui , Ng Kee Peng , Choo Siew Woh 
Published: July 2012

ABSTRACT

Mycobacterium massiliense
is a rapidly growing mycobacterial species. The pathogenicity of this subspecies is not well known. We report here the annotated genome sequence of
M. massiliense
strain M18, which was isolated from a lymph node biopsy specimen from a Malaysian patient suspected of having tuberculous cervical lymphadenitis.
Draft Genome Sequence of the Sulfur-Oxidizing Bacterium “Candidatus Sulfurovum sediminum” AR, Which Belongs to the Epsilonproteobacteria
Authors: Park Soo-Je , Ghai Rohit , Martín-Cuadrado Ana-Belén , Rodríguez-Valera Francisco , Jung Man-Young , Kim Jong-Geol , Rhee Sung-Keun 
Published: July 2012

ABSTRACT

Sulfur-oxidizing bacteria are common microorganisms in a variety of sulfide-rich environments. They play important roles in the global sulfur cycle on earth. Here, we present a high-quality draft genome sequence of a sulfur-oxidizing bacterium, “
Candidatus
Sulfurovum sediminum” strain AR, which belongs to the class
Epsilonproteobacteria
and dominated an enrichment culture from a marine sediment collected off Svalbard, within the Arctic Circle. Its genome contains genes for sulfur oxidation and carbon fixation. The size of the draft genome is 2.12 Mb, and the G+C content is 39.4%.
Draft Genome Sequence of Arctic Marine Bacterium Pseudoalteromonas issachenkonii PAMC 22718
Authors: Park Ha Ju , Shin Seung Chul , Kim Dockyu 
Published: July 2012

ABSTRACT

The psychrotolerant
Pseudoalteromonas issachenkonii
PAMC 22718 was isolated for its higher chitinase and protease activities from cold seawater in the Kara Sea, Arctic. Here, we present the draft genome sequence of PAMC 22718 to provide further information for the ecological function of the genus
Pseudoalteromonas
in a cold marine environment.
Genome Sequences of Two Thermophilic Bacillus licheniformis Strains, Efficient Producers of Platform Chemical 2,3-Butanediol
Authors: Li Lixiang , Su Fei , Wang Yu , Zhang Lijie , Liu Cuicui , Li Jingwen , Ma Cuiqing , Xu Ping 
Published: July 2012

ABSTRACT

Both
Bacillus licheniformis
strains 10-1-A and 5-2-D are efficient producers of 2,3-butanediol. Here we present 4.3-Mb and 4.2-Mb assemblies of their genomes. The key genes for the regulation and metabolism of 2,3-butanediol production were annotated, which may provide further insights into the molecular mechanism for the production of 2,3-butanediol with high yield and productivity.
Disk Diffusion and MIC Quality Control Ranges for BC-3205 and BC-3781, Two Novel Pleuromutilin Antibiotics
Authors: Ross James E. , Sader Helio S. , Ivezic-Schoenfeld Zrinka , Paukner Susanne , Jones Ronald N. 
Published: July 2012

ABSTRACT

MIC and disk diffusion quality control (QC) ranges were established for two new pleuromutilin antimicrobials (BC-3205 and BC-3781) in an eight-laboratory study performed according to Clinical and Laboratory Standards Institute M23-A3 guidelines.
Staphylococcus aureus
ATCC 29213 and 25923,
Streptococcus pneumoniae
ATCC 49619, and
Haemophilus influenzae
ATCC 4927 strains were evaluated. The proposed QC ranges would aid clinical laboratories in testing these compounds following their development for treatment of respiratory and cutaneous infections.
Analysis of Borderline Oxacillin-Resistant Staphylococcus aureus (BORSA) Strains Isolated in Tunisia
Authors: Maalej Senda Mezghani , Rhimi Faouzia Mahjoubi , Fines Marguerite , Mnif Basma , Leclercq Roland , Hammami Adnene 
Published: July 2012

ABSTRACT

Twenty-three strains of
Staphylococcus aureus
with borderline resistance to oxacillin were studied. These strains were not detected by the cefoxitin test, tests for penicillin-binding protein 2a (PBP2a),
mecA
, and
mecA
LGA251
were negative, and the strains were genetically unrelated. To detect all strains resistant to oxacillin, laboratories should routinely test for both cefoxitin and oxacillin.
Targeting both rs12979860 and rs8099917 Polymorphisms with a Single-Tube High-Resolution Melting Assay for
IL28B
Genotyping
Authors: Poon Kok Siong , Ho Sherry Sze Yee , Tang Julian Wei-Tze , Chua Cui Wen , Chiu Lily , Koay Evelyn Siew-Chuan 
Published: July 2012

ABSTRACT

A rapid, duplex, high-resolution melting interleukin-28B gene (
IL28B
) genotyping assay, targeting both rs12979860 and rs8099917 polymorphisms, was developed and validated using 30 DNA samples from healthy volunteers. A linkage study on 300 healthy Singaporeans showed variable haplotypes. When the assay was applied to plasma DNA from 50 hepatitis C virus genotype-1 (HCV-1)-infected patients, five compound heterozygous types were detected.
Moloney Leukemia Virus Type 10 Inhibits Reverse Transcription and Retrotransposition of Intracisternal A Particles
Authors: Lu Chunye , Luo Zeping , Jäger Stefanie , Krogan Nevan J. , Peterlin B. Matija 
Published: July 2012

ABSTRACT
Moloney leukemia virus type 10 protein (MOV10) is an RNA helicase that is induced by type I interferon. It inhibits HIV replication at several steps of its replicative cycle. Of interest, MOV10 is a component of mRNA processing (P) bodies, which inhibit retrotransposition (RTP) of intracisternal A particles (IAP). In this report, we studied the effects of MOV10 on IAP RTP and its dependence on P bodies. Indeed, MOV10 inhibited IAP RTP. It decreased significantly not only the products of reverse transcriptase but also its endogenous activity. MOV10 also associated with IAP RNA. Furthermore, although it was found in IAP virus-like particles, it did not affect their incorporation of IAP RNA, primer tRNAPhe (phenylalanine tRNA), or IAP Gag. Concerning P bodies, the exogenously expressed MOV10 had no effect on their size and number, and the inhibition of IAP RTP persisted despite the depletion of their RCK subunit. Thus, by interfering with reverse transcription, MOV10 inhibits IAP RTP, and this inhibition is independent of P bodies.
Construction of a Gammaretrovirus with a Novel Tropism and Wild-Type Replication Kinetics Capable of Using Human APJ as Entry Receptor
Authors: Bahrami Shervin , Pagh Kristina , Ejegod Ditte , Duch Mogens , Tolstrup Martin , Pedersen Finn Skou 
Published: July 2012

ABSTRACT
We have constructed a replication-competent gammaretrovirus (SL3-AP) capable of using the human G-protein-coupled receptor hAPJ as its entry receptor. The envelope protein of the virus was made by insertion of the 13-amino-acid peptide ligand for hAPJ, flanked by linker sequences, into one of the variable loops of the receptor binding domain of SL3-2, a murine leukemia virus (MLV) that uses the xenotropic-polytropic virus receptor Xpr1 and which has a host range limited to murine cells. This envelope protein can utilize hAPJ as well as murine Xpr1 for entry into host cells with equal efficiencies. In addition, the SL3-AP virus replicates in cells expressing either of its receptors, hAPJ and murine Xpr1, and causes resistance to superinfection and downregulation of hAPJ in infected cells. Thus, SL3-AP is the first example of a retargeted replication-competent retrovirus, with replication characteristics and receptor interference properties similar to those of natural isolates.
Posterior reversible encephalopathy syndrome postautologous peripheral stem cell transplantation for multiple myeloma
Authors: Zaman Shahriar Raj 
Published: July 2012

This is a case of a 56-year-old lady with recent autologous peripheral stem cell transplantation for multiple myeloma. She was presented with a 48 h history of worsening headache, drowsiness, nausea/vomiting and some peripheral neurological symptoms. She developed status epilepticus, was intubated and transferred to intensive care unit. After full investigation with a CT head, CT cerebral angiogram, MRI brain and cerebral angiogram, she was diagnosed with posterior reversible encephalopathy syndrome (PRES) with the help of expert opinion, based on the clinical and radiological evidence. The MRI showed bilateral occipital signal changes suggestive of PRES. She was managed with nimodipine, phenytoin and clonazepam with good effect. Eventually extubated, she made good progress on the ward with no further seizure episodes. Functionally she did not appear to have any evidence of residual damage from the PRES. This case discusses PRES on a background of a haematological malignancy and its clinical features.
Mapping of thecss(chloroplast splicing suppressor) gene(s) to a recombinationally suppressed region of chromosome III inChlamydomonas reinhardtii
Authors: Luo Liming , Lee Jaesung , Herrin David L. 
Published: July 2012

In previous work, three suppressors of defective group I introns (7151, 71N1, 7120) were isolated from a mutant of Chlamydomonas reinhardtii that had a splicing-deficient chloroplast large subunit (LSU) rRNA intron. Genetic analysis indicated that the 7151 and 71N1 suppressor mutations each involved single nuclear loci, and that the 7151 mutation was dominant. Here we present genetic evidence that the 7120 suppressor also involves a single nuclear locus and that the mutation is dominant in vegetative diploids. Moreover, we have employed crosses with the S1D2 strain and molecular markers to map the 7120 and 71N1 suppressors. Based on an analysis of 800 progeny from 7120 × S1D2, the 7120 suppressor is located in a region of ∼400 kb on chromosome III that is devoid of recombination. The ∼400-kb region contains at least 72 genes, about one-third of which (i.e., 22) are predicted to be organelle targeted. Similar analysis of 71N1 × S1D2 using 400 progeny also pointed to the recombination-deficient region of chromosome III, raising the possibility that these mutations could affect the same gene. These efforts lay the foundation for identifying the css (chloroplast splicing suppressor) gene(s), which promotes splicing of multiple chloroplast group I introns.
Nonlinear Dynamics of Two Western Boundary Currents Colliding at a Gap
Authors: Wang Zheng , Yuan Dongliang 
Published: July 2012

Abstract
The nonlinear collision of two western boundary currents (WBCs) of equal transport at a gap of the western boundary is studied using a 1.5-layer reduced-gravity quasigeostrophic ocean model. It is found that, when the gap (of width 2a) is narrow, a ≤ 7.3LM (LM the Munk thickness), neither of the WBCs can penetrate into the western basin because of the restriction of the viscous force. When 7.3LM &lt; a &lt; 9.0LM, both WBCs penetrate into the western basin for small transport and choke for large transport. When 9.0LM ≤ a ≤ 9.6LM, the two WBCs penetrate for small transport, choke for intermediate transport, and shed eddies periodically for large transport. When a &gt; 9.6LM, no steady choking state is found. Instead, the WBCs have only two equilibrium states: the penetrating and the periodic eddy shedding states. A Hopf bifurcation is found for a &gt; 9.0LM. The Reynolds number (Re) of the Hopf bifurcation is sensitive to the magnitude of γ(a/LM) and the baroclinic deformation radius, being small for larger γ or deformation radius. In addition, a reverse Hopf bifurcations is identified in the decreased Re experiments, occurring at a smaller Re than that of the Hopf bifurcation. The Re of the reverse Hopf bifurcation is not sensitive to the magnitude of the baroclinic deformation radius.
Hysteresis behavior of the WBCs is found for a &gt; 9.0LM, because of the existence of the Hopf and reverse Hopf bifurcations. In between them, steady penetrating or choking states coexist with eddy-shedding states. The steady states are found to be sensitive to perturbations of relative vorticity and can transit to periodic eddy-shedding states at the forcing of a mesoscale eddy.
Addiction to c-MYC in multiple myeloma
Authors: Holien Toril , Våtsveen Thea Kristin , Hella Hanne , Waage Anders , Sundan Anders 
Published: July 2012

Abstract
In multiple myeloma, c-MYC is activated and contributes to the malignant phenotype. Targeting MYC by short hairpin RNA induced cell death in myeloma cell lines; however, cell lines are generated from samples taken in advanced stages of the disease and may not reflect patient cells adequately. In this study, we used the selective small molecule inhibitor of MYC-MAX heterodimerization, 10058-F4, on myeloma cell lines as well as primary myeloma cells, and we show that inhibition of c-MYC activity efficiently induces myeloma cell death. Moreover, in cocultures of cell lines with bone marrow stromal cells from myeloma patients, the inhibitor still induces apoptosis. Our results provide further evidence that myeloma cells are addicted to c-MYC activity and that c-MYC is a promising therapeutic target in multiple myeloma.