bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interval training suppresses nod-like receptor protein 3 inflammasome activation to improve cardiac function in myocardial infarction rats by hindering the activation of the transforming growth factor-β1 pathway
Authors: Wei Wei , Xie Ping , Wang Xuemei 
Published: May 2024

Abstract
Objective
Myocardial infarction (MI) -induced cardiac dysfunction can be attenuated by aerobic exercises. This study explored the mechanism of interval training (IT) regulating cardiac function in MI rats, providing some theoretical basis for clarifying MI pathogenesis and new ideas for clinically treating MI.

Methods
Rats were subjected to MI modeling, IT intervention, and treatments of the Transforming growth factor-β1 (TGF-β1) pathway or the nod-like receptor protein 3 (NLRP3) activators. Cardiac function and hemodynamic indicator alterations were observed. Myocardial pathological damage and fibrosis, reactive oxygen species (ROS) level, superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities, MDA content, inflammasome-associated protein levels, and inflammatory factor levels were assessed. The binding between TGF-β1 and receptor was detected.

Results
MI rats exhibited decreased left ventricle ejection fraction (LVEF), left ventricle fractional shortening (LVFS), left ventricular systolic pressure (LVSP), positive and negative derivates max/min (dP/dt max/min) and increased left ventricular end-systolic pressure (LVEDP), a large number of scar areas in myocardium, disordered cell arrangement and extensive fibrotic lesions, increased TGF-β1 and receptor binding, elevated ROS level and MDA content and weakened SOD, CAT and GSH-Px activities, and up-regulated NLRP3, apoptosis-associated speck-like protein containing a CARD (ASC) and cleaved-caspase-1 levels, while IT intervention caused ameliorated cardiac function. IT inactivated the TGF-β1 pathway to decrease oxidative stress in myocardial tissues of MI rats and inhibit NLRP3 inflammasome activation. Activating NLRP3 partially reversed IT-mediated improvement on cardiac function in MI rats.

Conclusion
IT diminished oxidative stress in myocardial tissues and suppressed NLRP3 inflammasome activation via inactivating the TGF-β1 pathway, thus improving the cardiac function of MI rats.
Evaluation of diethyl 4-(5-bromo-1H-indol-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate: synthesis, anti-corrosion potential, and biomedical applications
Authors: Ahamed F. M. Mashood , Padusha M. Syed Ali , Banu A. Mushira , Maitra Swastika , Alharbi Hanan M. , Kumarasamy Vinoth , Uti Daniel E. , Mohite Popat , Alexiou Athanasios , Ali Iftikhar 
Published: May 2024

AbstractThe pursuit of advanced multifunctional compounds has gained significant momentum in recent scientific endeavours. This study is dedicated to elucidating the synthesis, rigorous characterization, and multifaceted applications—encompassing anti-corrosion, antimicrobial, and antioxidant properties—of Diethyl 4-(5-bromo-1H-indol-3-yl)-2,6-dimethyl-1,4-dihydropyridine-3,5-dicarboxylate. The 1,4-dihydropyridine derivative was meticulously synthesized through a strategic reaction of ethyl acetoacetate, ammonium acetate, and 5-bromoindole-3-carboxaldehydein the ethanol medium at 60 C. Subsequent spectral validations were conducted using sophisticated techniques, namely FTIR, NMR, and Mass spectrometry, resulting in data that perfectly resonated with the hypothesized chemical structure of the compound. Its anti-corrosive potential was assessed on mild steel subjected to an aggressive acidic environment, employing comprehensive methodologies like gravimetric analysis, Tafel polarization, and EIS. Concurrently, its antimicrobial prowess was ascertained against a spectrum of bacterial and fungal pathogens viz., Staphylococcus aureus, Bacillus subtilis, Escherichia coli, Pseudomonas, Candida albicansandAspergillusniger, leveraging the disc diffusion method and using Gentamicin as a reference standard.The empirical results illustrated a substantial decrement in corrosion rates with ascending concentrations of the organic compound, achieving an apex of anti-corrosive efficacy at 81.89% for a concentration of 2 × 103 M. Furthermore, the compound outperformed Gentamicin in antimicrobial screenings, manifesting superior efficacy against all tested pathogens. The antioxidant potential, quantified using the DPPH free radical scavenging assay against ascorbic acid as a benchmark, was found to have an IC50 value of 113.964 ± 0.076 µg/ml.This comprehensive investigation accentuates the paramount potential of the synthesized dihydropyridine derivative in diverse domains—from industrial applications as a corrosion inhibitor to therapeutic avenues given its pronounced antimicrobial and antioxidant capabilities. The compelling results obtained pave the way for expansive research and development initiatives cantered around this multifaceted compound.
Graphical Abstract
A single subcutaneous dose of eprinomectin (Eprecis®) is effective against common gastrointestinal nematodes and lungworms in experimentally infected lactating goats
Authors: Beck Alexandra , Thomson Sarah , Reddick David , Brunner Rike , Campbell-Wilson Dana , Achard Damien , Isaka Naomi , Trotel Anne , Karembe Hamadi 
Published: May 2024

Abstract
Background
The health and productivity of dairy goats continue to be impacted by gastrointestinal nematodes (GIN) and lungworms (LW). Eprinomectin (EPN) is frequently selected for treatment because it is generally effective and does not require a milk withdrawal period. However, some factors, such as lactation, can have an impact on EPN pharmacokinetics and potentially its efficacy. To evaluate whether this can alter the efficacy of Eprecis® 2%, an eprinomectin injectable solution, a study was performed in lactating goats using the dose currently registered in cattle, sheep and goats (0.2 mg/kg).

Methods
This study was a blinded, randomized, controlled trial performed according to the VICH guidelines. Eighteen (18) worm-free lactating goats were included and experimentally challenged on day 28 with a mixed culture of infective gastrointestinal and lung nematode larvae (Haemonchus contortus, Trichostrongylus colubriformis, Teladorsagia circumcincta, Dictyocaulus filaria). At D-1, fecal samples were collected to confirm patent infection in all animals. On D0, the goats were randomly allocated into two groups of nine goats; group 1 was treated with Eprecis® 2% at 0.2 mg/kg BW by subcutaneous injection, while group 2 remained untreated. Fecal samples for egg counts were collected from all animals on days 3, 5, 7, 9, 11 and 14. On D14, all goats were killed, and the abomasum, small intestine and lungs were removed, processed and subsampled to record the number and species of worms.

Results
The treatment was well tolerated. After treatment, the arithmetic mean FEC decreased in the treated group and remained < 5 EPG until the end of the study, while the arithmetic mean FEC in the control group remained > 849.0 EPG. At D14, goats in the treated group had very limited or zero total worm counts, whereas all animals from the control group had a high worm burden. The measured efficacy was 100.0% against H. contortus and T. colubriformis, 99.9% against T. circumcincta and 98.0% against D. filaria.

Conclusions
Eprinomectin (Eprecis®, 20 mg/ml), administered at the label dose (0.2 mg/kg), is highly effective against gastrointestinal nematodes and lungworms in lactating goats.

Graphical abstract
Efficacy and safety study of targeted small-molecule drugs in the treatment of systemic lupus erythematosus
Authors: Wang Shiheng , Ning Wanling , Tang Hanqing , Mu Chaochao , Huang Xiaosong 
Published: May 2024

Abstract
Background
Targeted small-molecule drugs in the treatment of systemic lupus erythematosus (SLE) have attracted increasing attention from clinical investigators. However, there is still a lack of evidence on the difference in the efficacy and safety of different targeted small-molecule drugs. Therefore, this study was conducted to assess the efficacy and safety of different targeted small-molecule drugs for SLE.

Methods
Randomized controlled trials (RCTs) on targeted small-molecule drugs in the treatment of SLE in PubMed, Web of Science, Embase, and Cochrane Library were systematically searched as of April 25, 2023. Risk of bias assessment was performed for included studies using the Cochrane’s tool for evaluating the risk of bias. The primary outcome indicators were SRI-4 response, BICLA response, and adverse reaction. Because different doses and courses of treatment were used in the included studies, Bayesian network meta-regression was used to investigate the effect of different doses and courses of treatment on efficacy and safety.

Results
A total of 13 studies were included, involving 3,622 patients and 9 targeted small-molecule drugs. The results of network meta-analysis showed that, in terms of improving SRI-4, Deucravacitinib was significantly superior to that of Baricitinib (RR = 1.32, 95% CI (1.04, 1.68), P < 0.05). Deucravacitinib significantly outperformed the placebo in improving BICLA response (RR = 1.55, 95% CI (1.20, 2.02), P < 0.05). In terms of adverse reactions, targeted small-molecule drugs did not significantly increase the risk of adverse events as compared to placebo (P > 0.05).

Conclusion
Based on the evidence obtained in this study, the differences in the efficacy of targeted small-molecule drugs were statistically significant as compared to placebo, but the difference in the safety was not statistically significant. The dose and the course of treatment had little impact on the effect of targeted small-molecule drugs. Deucravacitinib could significantly improve BICLA response and SRI-4 response without significantly increasing the risk of AEs. Therefore, Deucravacitinib is very likely to be the best intervention measure. Due to the small number of included studies, more high-quality clinical evidence is needed to further verify the efficacy and safety of targeted small-molecule drugs for SLE.
Faecal shedding of SARS-CoV-2 from patients with asymptomatic and mild COVID-19 without gastrointestinal symptoms in Ghana
Authors: Aninagyei Enoch , Ayivor-Djanie Reuben , Gyamfi Jones , Owuani Theodore , Ameke Selassie Louis , Kpeli Grace Semabia , Agbogli Hubert Kwame , Essandoh Priscilla , Duedu Kwabena Obeng 
Published: May 2024

Abstract
Objective
In this study, we sought to determine whether faecal shedding occurs among SARS-COV-2 positive Ghanaians, as reported elsewhere. Hence we assayed for SARS-COV-2 in the stools of 48 SARS-COV-2 confirmed patients at the Ho Municipal Hospital in Ghana.

Results
Of the 48 COVID-19 patients, 45 (93.8%) had positive tests for SARS-CoV-2 faecal shedding. About 60% reported no respiratory symptoms, while only 2% (1 patient) reported gastrointestinal (GI) symptoms in the form of nausea. Other symptoms reported included headache (57.9%), weakness (57.9%), cough (52.6%), blocked/runny nose (47.4%), fever (31.6%), sore throat (31.6%), and shortness of breath (21.1%). One person complained of nausea (5.3%) Semi-quantitative comparison of the SARS COV-2 viral loads in matched respiratory and faecal samples using the cycle threshold (CT) values revealed no statistical differences. Furthermore, the duration between collection of respiratory and faecal samples did not have any direct influence on the differences in the CT values. This suggests that treatment and use of sewage for environmental surveillance of SARS COV-2 could be a potential public health countermeasure.
An atlas of spider development at single-cell resolution provides new insights into arthropod embryogenesis
Authors: Leite Daniel J. , Schönauer Anna , Blakeley Grace , Harper Amber , Garcia-Castro Helena , Baudouin-Gonzalez Luis , Wang Ruixun , Sarkis Naïra , Nikola Alexander Günther , Koka Venkata Sai Poojitha , Kenny Nathan J. , Turetzek Natascha , Pechmann Matthias , Solana Jordi , McGregor Alistair P. 
Published: May 2024

AbstractSpiders are a diverse order of chelicerates that diverged from other arthropods over 500 million years ago. Research on spider embryogenesis, particularly studies using the common house spiderParasteatoda tepidariorum, has made important contributions to understanding the evolution of animal development, including axis formation, segmentation, and patterning. However, we lack knowledge about the cells that build spider embryos, their gene expression profiles and fate. Single-cell transcriptomic analyses have been revolutionary in describing these complex landscapes of cellular genetics in a range of animals. Therefore, we carried out single-cell RNA sequencing ofP. tepidariorumembryos at stages 7, 8 and 9, which encompass the establishment and patterning of the body plan, and initial differentiation of many tissues and organs. We identified 20 cell clusters, from 18.5 k cells, which were marked by many developmental toolkit genes, as well as a plethora of genes not previously investigated. We found differences in the cell cycle transcriptional signatures, suggestive of different proliferation dynamics, which related to distinctions between endodermal and some mesodermal clusters, compared with ectodermal clusters. We identified many Hox genes as markers of cell clusters, and Hox gene ohnologs were often present in different clusters. This provided additional evidence of sub- and/or neo-functionalisation of these important developmental genes after the whole genome duplication in an arachnopulmonate ancestor (spiders, scorpions, and related orders). We also examined the spatial expression of marker genes for each cluster to generate a comprehensive cell atlas of these embryonic stages. This revealed new insights into the cellular basis and genetic regulation of head patterning, hematopoiesis, limb development, gut development, and posterior segmentation. This atlas will serve as a platform for future analysis of spider cell specification and fate, and studying the evolution of these processes among animals at cellular resolution.
Magnetotelluric sampling theorem
Authors: Utada Hisashi , Rung-Arunwan Tawat , Siripunvaraporn Weerachai 
Published: May 2024

AbstractWe consider a general case of a magnetotelluric (MT) study to reveal three-dimensional (3D) distribution of the electrical conductivity within the Earth based on measurements of electromagnetic (EM) fields by a two-dimensional (2D) array. Such an MT array observation can be regarded as a spatially discrete sampling of the MT responses (impedances), and each observation site can be regarded as a sampling point. This means that MT array measurements must follow the Nyquist–Shannon sampling theorem. This paper discusses how the sampling theorem is applied to MT array studies and what kind of consideration is required in the application on the basis of synthetic model calculations, with special attention to spatial resolutions. With an aid of the EM scattering theory and the sampling theorem, we can show that an observation array resolves some features of the MT impedance but does not others. We call the resolvable and unresolvable features the MT signal and noise, respectively. This study introduces the spatial Fourier transform of array MT data (impedances) which helps us investigating sampling effects of lateral heterogeneity from a different angle (in the wavenumber domain). Shallow heterogeneities cause a sharp spatial change of impedance elements near structural boundaries. High wavenumber Fourier components are required to describe such a feature, which means the site spacing must be sufficiently short to be able to resolve such features. Otherwise, a set of array MT data will suffer from aliasing, which is one of the typical causes of MT distortion (MT geologic noise). Conversely, a signal due to a deep-seated conductivity anomaly will have more reduced amplitude at higher wavenumbers, which means focused imaging of such an anomaly is generally difficult. Finally, it is suggested to properly consider the sampling theorem in an observation array design, so as to have best performance in resolving MT signals.
Graphical abstract
Eye-tracking-based analysis of pharmacists’ thought processes in the dispensing work: research related to the efficiency in dispensing based on right-brain thinking
Authors: Tsuji Toshikazu , Nagata Kenichiro , Tanaka Masayuki , Hasebe Shigeru , Yukita Takashi , Uchida Mayako , Suetsugu Kimitaka , Hirota Takeshi , Ieiri Ichiro 
Published: May 2024

Abstract
Background
Pharmacists should be aware of their thought processes in dispensing work, including differences in the dispensing complexities owing to different drug positions in the left, center, and right areas. Dispensing errors associated with “same-name drugs (a pair of drugs with the same name but a different ingredient quantity)” are prevalent and often negatively affect patients. In this study, using five pairs of comparative models, the gaze movements of pharmacists in dispensing work were analyzed using an eye-tracking method to elucidate their thought processes.

Methods
We prepared verification slides and displayed them on a prescription monitor and three drug rack monitors. The dispensing information (drug name, drug usage, location display, and total amount) was displayed on a prescription monitor. A total of 180 drugs including five target drugs were displayed on the three drug rack monitors. Total gaze points in the prescription area, those in the drug rack area, total vertical movements between the two areas, and time required to dispense drugs were measured as the four classifications Gaze 1, Gaze 2, Passage, and Time, respectively. First, we defined the two types of location displays as “numeral combination” and “color/symbol combination.” Next, we defined two pairs of models A1-A2 (numerals) and B1-B2 (color/symbol) to compare differences between the left and right areas. Moreover, three pairs of models C1-C2 (left), D1-D2 (center), and E1-E2 (right) were established to compare differences between “numeral combination” and “color/symbol combination.”

Results
Significant differences in the complexities of dispensing work were observed in Gaze 2, Passage, and Time between the models A1-A2 (A1<A2), in Gaze 2 between the models B1-B2 (B1>B2), and in Gaze 2 and Time between the models C1-C2, D1-D2, and E1-E2 (C1>C2, D1>D2, and E1>E2, respectively).

Conclusions
Using the current dispensing rules, pharmacists are not good at dispensing drugs located in the right area. An effective measure for reducing the dispensing complexity is to introduce visual information in the prescription content; the utilization of the right brain facilitates reducing the complexity in the right dispensing area.
Evaluating the impact of school-based influenza vaccination programme on absenteeism and outbreaks at schools in Hong Kong: a retrospective cohort study protocol
Authors: Miao Chuhan , Lu Qingyang , Wu Yuqian , He Jianxun 
Published: May 2024

Abstract
Introduction
Seasonal influenza causes annual school breaks and student absenteeism in Hong Kong schools and kindergartens. This proposal aims to conduct a retrospective cohort study to evaluate the impact of a school-based influenza vaccination (SIV) programme on absenteeism and outbreaks at schools in Hong Kong.

Methods
The study will compare schools that implemented the SIV programme with schools that did not. The data will be sourced from school records, encompassing absenteeism records, outbreak reports, and vaccination rates. We will recruit 1000 students from 381 schools and kindergartens in 18 districts of Hong Kong starting June 2024. The primary outcome measures will include absenteeism rates due to influenza and school influenza outbreaks. Secondary outcomes will consist of vaccination coverage rates and the impact of the SIV programme on hospitalisations due to influenza-like illness. A t-test will be conducted to compare the outcomes between schools with and without the SIV programme.

Ethics and dissemination
The school completed signing the participants’ informed consent form before reporting the data to us. Our study has been approved by the Hospital Authority Hong Kong West Cluster IRB Committee (IRB No: UW 17–111) and was a subtopic of the research “The estimated age-group specific influenza vaccine coverage rates in Hong Kong and the impact of the school outreach vaccination program”.

Trial registration
This study will be retrospectively registered.
Efficient and Flexible Long-Tail Recommendation Using Cosine Patterns
Authors: Wang Yaqiong , Wu Junjie , Wu Zhiang , Adomavicius Gediminas 
Published: May 2024

With the increasing use of recommender systems in various application domains, many algorithms have been proposed for improving the accuracy of recommendations. Among various dimensions of recommender systems performance, long-tail (niche) recommendation performance remains an important challenge in large part because of the popularity bias of many existing recommendation techniques. In this study, we propose CORE, a cosine pattern–based technique, for effective long-tail recommendation. Comprehensive experimental results compare the proposed approach with a wide variety of classic, widely used recommendation algorithms and demonstrate its practical benefits in accuracy, flexibility, and scalability in addition to the superior long-tail recommendation performance. 1 History: Accepted by Ramaswamy Ramesh, Area Editor for Data Science & Machine Learning. Funding: This work was supported by the National Natural Science Foundation of China [Grants 72031001, 72072091, 72242101]. Supplemental Material: The software that supports the findings of this study is available within the paper and its Supplemental Information ( https://pubsonline.informs.org/doi/suppl/10.1287/ijoc.2022.0194 ) as well as from the IJOC GitHub software repository ( https://github.com/INFORMSJoC/2022.0194 ). The complete IJOC Software and Data Repository is available at https://informsjoc.github.io/ .
pedagogia decolonial e a interculturalidade crítica para o/no ensino de português como língua adicional no contexto multilíngue e multicultural da Guiné-Bissau
Authors: Quessana M'bana Fidel , Valênia Martins Dias Anair 
Published: May 2024

Este artigo objetiva apresentar e propor reflexões sobre o ensino do português como língua adicional (PLA), à luz da pedagogia decolonial e da interculturalidade crítica, no contexto da diversidade linguística e cultural da Guiné-Bissau, como forma de contribuir com a melhoria da qualidade do ensino guineense e sua adequação ao perfil linguístico e cultural do alunado. Teoricamente, o trabalho está apoiado nos estudos da pedagogia decolonial e a interculturalidade crítica (WALSH, 2018; OLIVEIRA; CANDAU, 2010), entre outros. No que diz respeito ao ensino de língua portuguesa (ANTUNES, 2003; MENDES, 2011), reflete-se sobre o ensino contextualizado de gramática e da formação de professores no contexto intercultural e multilíngue. A metodologia da pesquisa baseou-se na abordagem qualitativa, tendo como procedimentos a pesquisa bibliográfica e documental. No bojo dessas reflexões, parece emergir que o ensino da língua adicional, numa perspectiva da pedagogia decolonial e da interculturalidade crítica, pode contribuir com a valorização da realidade cultural e linguística dos alunos, bem como com um ensino reflexivo-crítico que forma cidadãos aptos a responderem as demandas de uma sociedade local e globalizada, cuja dinâmica exige o desenvolvimento da competência e habilidade de falar, negociar e tomar decisões adequadas ao contexto no qual o sujeito está inserido.
Palavras-chave: Ensino da língua. Pedagogia decolonial. Interculturalidade crítica.