bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modified Arthroscopic Repair of a Posterior Cruciate Ligament Tibial Avulsion Fracture Improves IKDC and Lysholm Score Compared to Open Reduction
Authors: Li Xingxing , Ma Qiming , Zheng Quan , Dou Qiangbing , Zhou Liang , Sun Liangye , Shao Song , Wang Qiwei 
Published: April 2024

Abstract

Purpose: The purpose of this study was to analyse the difference between arthroscopic fixation and open reduction internal fixation (ORIF) of posterior cruciate ligament (PCL) tibial avulsion fractures.
Methods: This retrospective study analysed patients with an acute PCL tibial avulsion fracture who underwent surgical treatment at our hospital and follow-up for at least 24 months. Variables based on sex, age, Meyers-McKeever type, surgical method, meniscus tear, external fixation, labour or sports, Lysholm knee score, IKDC score, and KT-1000 value were also recorded. Multifactor unconditional logistic regression and Student’s t test with 1:1 propensity score matching (PSM) to remove confounding factors were used for analysis.
Results: Sixty-five cases achieved knee function graded as “good” or better, and 9 cases not. Single-factor analysis indicated that Meyers-McKeever type (c2=4.669, P=0.031) and surgical approach (c2=9.428, P=0.002) are related to functional outcomes. Multifactorial logistic regression analysis further confirmed that Meyers-McKeever typing (OR=10.763, P=0.036, [95% CI, 1.174-98.693]) and surgical approach (OR=9.274, P=0.008, [95% CI, 1.794-47.934]) are independent risk factors affecting prognosis. In addition, PSM verified significant differences in the Lysholm score (t=3.195, P=0.006), IKDC score (t=4.703, P=0.000) and A-KT/H-KT (t=2.859, P=0.012). However, the affected-side KT-1000 value (A-KT, mm, t=1.225, P=0.239) and healthy-side KT-1000 value (H-KT, mm, t=1.436, P=0.172) did not significantly differ between the two groups. The proportions of cases in which the Lysholm score, IKDC and A-KT/H-KT exceeded the minimal clinically important difference (MCID) were 62.5% (20/32), 62.5% (20/32) and 93.75% (30/32), respectively.
Conclusion: Compared with ORIF, an arthroscopic approach for PCL tibial avulsion fractures achieves better results.
Level of evidence: Retrospective cohort study; Level II.
Laparoscopic repeat liver resection for recurrent hepatocellular carcinoma: when should we start it ?
Authors: Chen YiChan , Soong Ruey-Shyang , Chiang Po-Hsing , Chai Shion Wei , Chien Chih-Ying 
Published: April 2024

Abstract

Introduction:
Hepatocellular carcinoma (HCC) prevails in Taiwan, primarily attributed to the high incidence of hepatitis B and hepatitis C infections with high recurrent rates of 50-70% within five years after initial treatment. When confronted with recurrent HCC, treatment options include salvage liver transplantation, trans-arterial chemo-embolization (TACE), re-hepatectomy, and radio-frequency ablation (RFA). Notably, repeat hepatectomy exhibits superior oncological outcomes compared to alternative approaches. While laparoscopic liver resection (LLR) has demonstrated safety and feasibility in primary HCC resection, the persistence of intra-hepatic recurrence necessitates effective interventions. However, repeat liver resection posed challenges, including adhesions from previous surgeries, limited access to recurrent tumors, altered liver structure post-regeneration, difficulties in obtaining hilar control, and compromised liver reserve. The suggestion of laparoscopic approach for recurrent HCC typically based on the surgeons’ experience and confidence.
Patients and methods:
We retrospectively review our collective experience spanning January 2009 to December 2021, encompassing 57 patients with recurrent HCC. Among them, 37 patients underwent laparoscopic approaches, while 20 patients opted for traditional procedures.
Results:
Notably, both groups exhibited similar operative times and perioperative outcomes, with significantly reduced hospital stays observed in the laparoscopic cohort (median: 5 vs 7, p<0.001). The introduction of laparoscopic techniques also sparked a strategy shifting in our surgical approach to recurrent HCC.
Conclusion:
Our manuscript aims to delineate a stepwise approach for navigating the challenges inherent in repeat operations, elucidating techniques for peritoneal cavity entry, meticulous adhesiolysis, effective Pringle maneuver application, tumor identification, and pertinent insights into perioperative outcomes. Under the stepwise approach, laparoscopic repeat liver resection can be performed safely and effectively with low incidence of conversion in a mature surgical team. This treatment choice should be offered to the patient in a mature surgical team of minimal invasive liver resection.
Platelet-to-lymphocyte ratio as a prognostic marker for advanced hepatocellular carcinoma treated with immune checkpoint inhibitors: Meta-analysis
Authors: Zhao Tong , Aimaijiang Gulizere , Zhang Yu , Zheng Yi 
Published: April 2024

Abstract

Background: The issue of platelet-to-lymphocyte ratio (PLR) may serve as proxy marker for the effectiveness of immune checkpoint inhibitors (ICI) in treating hepatocellular carcinoma (HCC) remains unresolved. This meta-analysis aims to investigate and provide insights into this issue.
Methods: We looked up potentially related articles in the Cochrane Library, Web of Science, PubMed and Embase up to December 2022. The correlation of PLR with progression free surviva(PFS) and overall survival（OS）were evaluated by Hazard ratios(HRS) and 95% confidence intervals(CIS). The heterogeneity of the different PLR groups and their potential predictive value in OS and PFS was measured through subgroup analysis.
Results: Ten studies were included in our study, which involving 1590 patients with advanced HCC who were received treatment with ICIs. In summary, the meta-analysis demonstrated that a significant correlation between high PLR and adverse OS,as well as PFS. The combination of these findings suggests that high PLR may adversely affect the effectiveness of cancer patients treated with ICIs. Patients who had a high PLR had a significant reduction in OS and PFS compared to a population with a low PLR. Sensitivity analysis showed similar results.
Conclusion: Overall, the advanced HCC patients with low PLR who were treated with ICIs, had better OS and PFS. PLR can predict the outcome of treatment with ICIs in patients with advanced HCC.
Cryopreservation and transplantation of spermatogonia stem cells in piracanjuba Brycon orbignyanus (Characiformes: Characidae), an endangered fish species
Authors: López Lucia Suárez , Monzani Paulo Sérgio , Carvalho Gabriella Braga , Silva Diógenes Henrique Siqueira , Vianna Norberto Castro , Yasui George Shigueki , Senhorini José Augusto 
Published: April 2024

Abstract

Piracanjuba (Brycon orbignyanus) is an endangered fish species from the Neotropical region. The establishment of a cryobank using spermatogonial stem cells (SSCs) and subsequent production of a germline chimera is thus a promising strategy for such species. In the present work, procedures for the isolation and cryopreservation of piracanjuba SSCs and subsequent transplantation into sterile recipients were established. The piracanjuba SSCs were obtained by Percoll density gradient centrifugation and differential plating. SSC fractions were evaluated by relative ddx4 expression, alkaline phosphatase activity, and light microscopy. SSC cryopreservation was performed using five cryoprotectants at three different concentrations. The mix of the cells from the 20% and 30% Percoll density gradients showed 58.35 ± 0.03% purity of SSCs. The purity of SSCs increased to 66.00 ± 0.01% after differential plating. The relative ddx4 expression was 3.5 times higher in cells from the Percoll density gradient centrifugation than in the gonad and cells after differential plating. Propanediol (1M) was the most effective cryoprotector evaluated (P = 1.000), showing 90.75 ± 1.85% cell viability. Freshly isolated and cryopreserved cells from the Percoll density gradient centrifugation were transplanted into a sterile male adult triploid hybrid with germ cell-less gonads. SSCs were observed in the germinal epithelium of the testes of recipients 20 days after transplantation. The results are promising for obtaining functional germline chimeras in Neotropical fish. Consequently, the procedures established here can be applied in future actions for the conservation and reconstitution of the piracanjuba in case of extinction.
Arabidopsis AGB1 participates in salinity response through bZIP17-mediated unfolded protein response
Authors: Cho Yueh 
Published: April 2024

Abstract

Background
Plant heterotrimeric G proteins respond to various environmental stresses, including high salinity. It is known that Gβ subunit AGB1 functions in maintaining local and systemic Na+/K+ homeostasis to accommodate ionic toxicity under salt stress. However, whether AGB1 contributes to regulating gene expression for seedling’s survival under high salinity remains unclear.
Results
We showed that AGB1-Venus localized to nuclei when facing excessive salt, and the induction of a set of bZIP17-dependent salt stress-responsive genes was reduced in the agb1mutant. We confirmed both genetic and physical interactions of AGB1 and bZIP17 in plant salinity response by comparing salt responses in the single and double mutants of agb1 and bzip17 and by BiFC assay, respectively. In addition, we show that AGB1 depletion decreases nuclei-localization of transgenic mRFP-bZIP17 under salt stress, as shown in s1p s2p double mutant in the Agrobacteria-mediated transient mRFP-bZIP17 expression in young seedlings.
Conclusions
Our results indicate that AGB1 functions in S1P and/or S2P-mediated proteolytic processing of bZIP17 under salt stress to regulate the induction of salinity-responsive gene expression.
Comparative Analysis of Intra-canal Photodynamic Therapy With Different Photosensitizers on Periapical Healing
Authors: ARI Reyhan Ece , AYDIN Uğur 
Published: April 2024

Abstract

Objectives: Various additional methods, such as photodynamic therapy (PDT), have been used to optimize the success of root canal treatment. This in vivo study aims to evaluate the effect of PDT with different photosensitizers on healing.
Materials and Methods: Seventy single canalled teeth with periapical lesions of 53 patients were included. After biomechanical preparation, they were randomly divided into four groups: control, methylene blue, curcumin, and erythrosine. The photosensitizers were ultrasonically activated for 1 minute after 3 minutes of pre-irradiation time (PIT). Afterward, they were irradiated with a diode laser for 1 minute. Radiographs at baseline, 1st, 3rd, 6th, 9th and 12th months were taken. Post-operative pain was recorded according to the VAS. The lesion dimensions were measured.
Results: Unlike the control group, there was a significant improvement between the baseline to the 1st month and the 1st month to the 3rd month in the PDT groups. At the twelfth month, mean lesion sizes decreased by 86% in the erythrosine and control groups, while 91% reduction was observed in the methylene blue and curcumin groups.
Conclusion: All lesions healed, but teeth treated with PDT healed faster in the first three months. According to the present results, PDT is an appropriate adjunct to conventional root canal treatment.
Clinical Relevance: The present study is the first in-vivo study including erythrosine-B and natural product such as curcumin in intra-canal photodynamic therapy. It will provide a valuable contribution in canal disinfection concept as an adjunct to routine irrigation.
Social Environment Related to Opportunities for Physical Activity within Different types of Built Environments: A Scoping Review
Authors: Høyer-Kruse Jens , Schmidt Eva Berthelsen , Hansen Anne Faber , Pedersen Marlene Rosager Lund 
Published: April 2024

Abstract

Background
The association between social and built environments plays a crucial role in influencing physical activity levels. However, a thorough understanding of their combined impact remains unclear. This scoping review seeks to clarify the interplay between social environments and opportunities for physical activity within different built environments, with a particular focus on the implications of socioeconomic status and urban planning on physical activity participation.
Methods
We conducted a systematic literature search across several databases to identify studies exploring the associations between social factors, built environment characteristics, and physical activity levels. The inclusion criteria were studies published in English between 2000 and 2022, encompassing urban, suburban, and rural contexts. Thematic analysis was employed to categorise studies based on the specific aspects of the built environment they investigated (walkability, cycling infrastructure, parks and open spaces, and sports facilities) and the social determinants they examined.
Results
A total of 72 studies were included in the review, illustrating a multifaceted relationship between access to physical activity opportunities and social determinants such as socioeconomic status, community engagement, and urban design. The findings highlighted that individuals from higher socioeconomic backgrounds typically enjoyed better access to a broad range of physical activity infrastructures than those from lower socioeconomic backgrounds, who encountered greater access barriers. Effective urban planning was identified as crucial in providing expanded physical activity opportunities, notably through more pedestrian-friendly environments, comprehensive cycling infrastructure, and accessible green spaces and sports facilities.
Conclusions
This review emphasises the significant impact of socioeconomic status and urban planning on access to physical activity opportunities. This underscores the necessity for urban planning policies to adopt an inclusive approach, considering the varied needs of different population groups to ensure equitable access to physical activity resources. Such strategies are crucial for public health initiatives aimed at enhancing physical activity levels across diverse community sectors, offering a potential avenue to alleviate health disparities associated with inactivity.
Epidemiology of Motor Vehicle Accident-Associated Ocular Trauma
Authors: Bhatnagar Nishay V. , Uppuluri Aditya , Bhagat Neelakshi , Langer Paul D. 
Published: April 2024

Abstract

Purpose
The objective is to investigate trends in cases of motor vehicle accident-associated (MVA-associated) ocular trauma in which the patient was the driver of the motor vehicle.
Methods
The study utilizes data from the 2007–2014 National Trauma Databank (NTDB), a national trauma registry. Status as the driver of the motor vehicle was identified using E-Codes from the International Classification of Diseases, Ninth Revision, Clinical Modification (ICD-9-CM). Trauma diagnoses were identified using D-Codes from the ICD-9-CM. Statistics were performed using IBM SPSS Version 23.
Results
We identified 49,660 cases of ocular trauma secondary to an MVA. Men comprised 68.6% (34,057) of cases. Majority of injuries (55.2%) occurred in the 18-40-year age group. Orbital floor fractures (OFFs) were the most commonly observed ocular injury, occurring in 17,647 (35.5%) cases and was highest in the 41-60-year age group (37.4%). There were 2,787 cases of open globe injury (OGI) with the highest proportion of cases in the 81 + age group (7.1%). OGIs were seen in 3.0% of cases with OFFs vs. 7.1% in those without. The mortality rate was 4.3%; the rate was highest in the 81 + age group (13.5%) and lowest in the 18–40 age group (3.3%).
Conclusion
Men and young adults comprised the majority of cases of MVA-associated ocular trauma. OFFs were seen in approximately one-third of cases of ocular trauma. OGIs were less commonly observed when a concurrent OFF was observed. Though the overall mortality was 4.3%, there was significant variation by age group.
Stigma and glume characteristics synergistically determine the stigma exsertion rate in thermo-photo sensitive genic male sterile wheat
Authors: Li Hongsheng , Yang Zhonghui , Li Shaoxiang , Elafnah Ahmed , Abdelkhalik Sedhom , Tang Xiong , Yin Jian , Ding Mingliang , Liu Kun , Yang Mujun , Wang Xiue 
Published: April 2024

Abstract

Background: Wheat hybrids have been widely demonstrated to have remarkable heterosis or hybrid vigor in increasing yield potential and stability since the 1960s. However, commercial application of hybrid wheat is still hindered by higher seed costs mostly resulting from lower yield in hybrid seed production. Stigma exsertion has been verified as a decisive factor in increasing rice's hybrid seed yield but has been nearly ignored in hybrid wheat breeding and seed production.
Results: In this study, four thermo-photo sensitive genic male sterile lines K41S, K64S, K66S and K68S with different stigma exsertion rate (SER) were used to compare their differences in floral architecture relative to stigma exsertion over two growing seasons. K41S and K64S exhibited relatively higher SER at 21.87% and 22.81%, respectively. No exserted stigma was observed in K66S, and K68S had only 0.82% SER. The study found that stigma length, glume width and the length-width ratio of the glume were significantly correlated with SER, with the correlation coefficients of 0.46, -0.46 and 0.60, respectively. Other stigma features such as branch angle, stretch width and hairbrush length, as well as glume length, also had a weakly positive correlation with SER (r = 0.09-0.27). For K41S and K64S, the SER was significantly affected by the differences in stigma branch angle and stigma stretch width among florets. A cross-pollination survey showed that the outcrossing ability of florets with exserted stigma was about 3 times as many as that of florets with non-exserted stigma. As a result, the stigma exserted florets that accounted for 21.87% and 22.81% of the total florets in K41S and K64S, produced 46.80% and 48.53% of the total cross-pollinated seeds in both sterile lines.
Conclusions: These findings suggest that the SER in wheat sterile line is synergistically determined by specific stigma and glume characteristics. Longer stigma combined with slender glume appears to be the basic floral feature of stigma exsertion. It is expected that breeding and utilizing sterile lines with higher SER would be a promising solution to cost-effective hybrid wheat seed production.
The effects of dietary intervention and MAF supplementation on cognitive function in elderly users of outpatient rehabilitation
Authors: Uchiyama-Tanaka Yoko , Yamakage Hajime , Inui Toshio 
Published: April 2024

Abstract

Backgroud: Age, genetic and environmental factors are noted to contribute to dementia risk. Neuroplasticity, protection from degeneration and cell death, and early intervention are desirable for preventing dementia. The linkage between neurons and microglia has been a research focus. In this study, we examined the effects of dietary modification (a reduction in advanced glycation end products [AGEs]) and macrophage activating factor (MAF; a macrophage regulator) supplementation on cognitive function in elderly participants undergoing rehabilitation.
Methods: Forty-three rehabilitation facility users aged over 60 years were randomly divided into three groups: a control group receiving placebo, a group receiving dietary guidance, and a group receiving dietary guidance and MAF supplementation. The amyloid-β40/42 ratio, dietary AGE intake, plasma AGE levels, dietary calorie intake and MCI screen as cognitive functions of participants were evaluated.
Results: Four participants withdrew from the study. MCI screen scores significantly improved in MAF supplementation group especially after 6 months. Dietary modulation was also effective for improving cognitive function after 12 months compared to placebo. Only control group significantly increased plasma AGE and worsened, dietary modulation and MAF supplementation group showed no change after 12 months.
Conclusions: MAF supplementation is effect for improvement of cognitive function especially after 6 months in elderly people with rehabilitation. Dietary modulation was also effective for cognitive function after 12 months compared to control group. Dietary guidance in day-care center rehabilitation was difficult due to conflicts with family members and the use of facilities. However, enthusiastic users showed improvement in cognitive function. In addition to exercise and dietary improvement, social involvement was found to be important again.
Trial registration: UMIN 000050155, 2023/02/14
Perturbation of periodic spot-generation balance leads to diversified pigmentation patterning of harlequin Phalaenopsis orchids: in silico prediction
Authors: Lu Ti-Wen , Chen Wen-Huei , Chen Pao-Yang , Shu Yu-Chen , Chen Hong-Hwa 
Published: April 2024

Abstract

Harlequin Phalaenopsis orchids are known for unprecedented clownish dark reddish-purple patches. The unreproducible diversified pigmentation patterning of harlequin flowers is an obstacle to commercialization. Insertion of a retrotransposon HORT1 in the promoter of a transcription activator for spotted pigmentation, PeMYB11, explains the patterning diversity. Nonetheless, in the absence of solo-LTR of HORT1, a transcriptional enhancer of PeMYB11, purple patches can still be generated in the presence of full-length HORT1, a transcriptional silencer of PeMYB11. Intriguingly, PeMYBx, a repressor for anthocyanin accumulation, is highly enriched in the purple region. Meanwhile, miR858 significantly expresses in the white region of harlequin flowers. To fill the knowledge gap, we refined the biological model for generating anthocyanin spots in Phalaenopsis, and then mathematically modeled the interactions with additional considerations of full-length HORT1 and the solo-LTR of HORT1, and altered amount of miR858. Our simulation suggests that not only the solo-LTR of HORT1 but also the reduced amount of miR858 take charge of the generation of purple patches but in an antagonistic manner. Our work extends the current understanding of how HORT1 and miR858 are involved in regulating the pigmentation patterning of harlequin Phalaenopsis and provides an experimental framework for future research.