bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pelvic floor muscle training for urinary incontinence in older adults: a best practice implementation project
Authors: SIC Terese Kochuvilayil , Cherian Sunu Alice , Vargese Saritha Susan , McArthur Alexa , Lizarondo Lucylynn 
Published: May 2024

ABSTRACT

Objectives:
This project aimed to implement best practices for pelvic floor muscle training to manage urinary incontinence among older women in long-term care in Kerala, India.

Introduction:
Urinary incontinence is a prevalent and distressing condition that affects a significant proportion of older adults and is characterized by involuntary loss of urine, leading to social embarrassment, decreased quality of life, and increased health care costs. It is more prevalent in women and is associated with dementia, limited mobility, and other comorbidities in long-term care. Pelvic floor muscle training is a first-line treatment option for urinary incontinence in older adults, given its potential to improve quality of life and reduce health care costs.

Methods:
This project was based on the JBI Evidence Implementation Framework. A baseline audit was conducted to evaluate current practice against best practices. After identifying barriers and implementing strategies, follow-up audits were conducted after 3 and 6 months.

Results:
The baseline audit showed 0% compliance with all best practices. Barriers such as lack of knowledge and practice of pelvic floor exercises for urinary incontinence among participants and nurses; unknown cognitive status; and health emergencies were identified. Strategies including video-assisted training of pelvic floor muscle exercises, training calendars, and flip charts with instructions. The follow-up audits showed significant improvements in compliance.

Conclusions:
This project reduced urinary incontinence in the participants. Although two of the audit criteria did not reach 100% compliance by the end of 6 months, the stakeholders of the long-term care facility understood the importance of pelvic floor muscle training, which will be beneficial for future residents with urinary incontinence.

Spanish abstract:

http://links.lww.com/IJEBH/A211
Catenovulum adriaticum sp. nov., isolated from algae in the harbour of Susak, Croatia
Authors: Schoch Teresa , Reich Sebastian J. , Flaiz Maximilian , Stöferle Sophia , Riedel Christian U. , Dürre Peter 
Published: May 2024

The use of algae as feedstock for industrial purposes, such as in bioethanol production, is desirable. During a search for new agarolytic marine bacteria, a novel Gram-stain-negative, strictly aerobic, and agarolytic bacterium, designated as TS8T, was isolated from algae in the harbour of the island of Susak, Croatia. The cells were rod-shaped and motile. The G+C content of the sequenced genome was 38.6 mol%. Growth was observed at 11–37 °C, with 0.5–13 % (w/v) NaCl, and at pH 6.0–9.0. The main fatty acids were summed feature 3 (C16 : 1
ω6c and/or C16 : 1
ω7c), summed feature 8 (C18 : 1
ω7c and/or C18 : 1
ω6c), and C16 : 0. The main respiratory quinone was ubiquinone-8. The major polar lipids were phosphatidylethanolamine and phosphatidylglycerol. Analysis of 16S rRNA gene sequences indicated that the newly isolated strain belongs to the genus Catenovulum. Based on 16S rRNA gene sequence data, strain TS8T is closely related to Catenovulum sediminis D2T (95.7 %), Catenovulum agarivorans YM01T (95.0 %), and Catenovulum maritimum Q1T (93.2 %). Digital DNA–DNA hybridization values between TS8T and the other Catenovulum strains were below 25 %. Based on genotypic, phenotypic, and phylogenetic data, strain TS8T represents a new species of the genus Catenovulum, for which the name Catenovulum adriaticum sp. nov. is proposed. The type strain is TS8T (=DSM 114830T=NCIMB 15451T).
Transformation of meiotic drive into hybrid sterility in Drosophila
Authors: Bladen Jackson , Nam Hyuck-Jin , Phadnis Nitin 
Published: May 2024

AbstractHybrid male sterility is one of the fastest evolving intrinsic reproductive barriers between recently isolated populations. A leading explanation for the evolution of hybrid male sterility involves genomic conflicts with meiotic drivers in the male germline. There are, however, few examples directly linking meiotic drive to hybrid sterility. Here, we report that theSex-Ratiochromosome ofDrosophila pseudoobscura, which causes X-chromosome drive within the USA subspecies, causes near complete male sterility when moved into the genetic background of the Bogota subspecies. In addition, we show that this new form of sterility is genetically distinct from the sterility of F1 hybrid males in crosses between USA males and Bogota females. Our observations provide a tractable study system where non-cryptic drive within species is transformed into strong hybrid sterility between very young subspecies.
Ultrasound-actuated drug delivery with acoustic percolation switches
Authors: Abundo Maria Paulene , Tifrea Anna T. , Buss Marjorie T. , Barturen-Larrea Pierina , Jin Zhiyang , Malounda Dina , Shapiro Mikhail G. 
Published: May 2024

ABSTRACTDevices that can be remote-controlled under image guidance to precisely deliver biomedicines to sites of disease are a major goal of biomedical research. However, most existing externally triggered delivery systems are based on complex micromachines that are controlled with electromagnetic waves and require custom external instrumentation. Here we present a drug delivery platform comprising a simple protein-containing hydrogel that can be both imaged and triggered to release drugs at specific locations using widely available ultrasound imaging devices. This technology is based on the addition of air-filled protein nanostructures called gas vesicles (GVs) to hydrogel delivery vehicles. While intact, GVs sterically block the release of drug payloads and allow the vehicle to be imaged with ultrasound. An increase in ultrasound pressure causes the collapse of GVs within hydrogels present at the desired anatomical location, instantly creating percolation channels and triggering rapid drug release. Both the imaging and release are performed using a common diagnostic ultrasound probe. We implement this concept by establishing ultrasound-controlled drug diffusion and release from hydrogelsin vitroand demonstrating targeted image-guided protein deliveryin vivofollowing oral administration. We use this approach to deliver anti-inflammatory antibodies to treat gastrointestinal inflammation in a rat model of colitis. Targeted acoustic percolation switches (TAPS) open a conduit for local, image-guided drug delivery with a simple formulation and commonplace ultrasound equipment.
An integrated picture of the structural pathways controlling the heart performance
Authors: Morotti Ilaria , Caremani Marco , Marcello Matteo , Pertici Irene , Bianco Pasquale , Narayanan Theyencheri , Piazzesi Gabriella , Reconditi Massimo , Lombardi Vincenzo , Linari Marco 
Published: May 2024

ABSTRACTRegulation of heart function is attributed to a dual filament mechanism: (i) the Ca2+-dependent structural changes in the regulatory proteins of the thin, actin-containing filament making actin available for myosin motor attachment1, and (ii) the release of motors from their folded (OFF) state on the surface of the thick filament2allowing them to attach and pull the actin filament. Thick filament mechanosensing is thought to control the number of motors switching ON in relation to the systolic performance3, but its molecular basis is still unknown. Here high spatial resolution X-ray diffraction data from electrically paced rat trabeculae and papillary muscles call for a revision of the mechanosensing hypothesis and provide a molecular explanation of the modulation of heart performance also in light of the recent cryo-EM thick filament structure4, 5. We find that upon stimulation titin activation6triggers structural changes in the thick filament that switch motors ON throughout the filament within ∼½ the maximum systolic force. These structural changes also drive MyBP-C N-terminus to bind actin7promoting first motor attachments from the central 1/3 of the half-thick filament. Progression of attachments towards the periphery of half-thick filament with increase in systolic force is carried on by near-neighbour cooperative thin filament activation by attached motors8. The identification of the roles of MyBP-C, titin, thin and thick filaments in heart regulation enables their targeting for potential therapeutic interventions.
Activation of Piezo1 Inhibits Kidney Cystogenesis
Authors: Fan Qingfeng , Hadla Mohamad , Peterson Zack , Nelson Grace , Ye Hong , Wang Xiaofang , Mardirossian Jean Marc , Harris Peter C. , Alper Seth L. , Prakash Y.S. , Beyder Arthur , Torres Vicente E. , Chebib Fouad T. 
Published: May 2024

AbstractThe disruption of calcium signaling associated with polycystin deficiency has been proposed as the primary event underlying the increased abnormally patterned epithelial cell growth characteristic of Polycystic Kidney Disease. Calcium can be regulated through mechanotransduction, and the mechanosensitive cation channel Piezo1 has been implicated in sensing of intrarenal pressure and in urinary osmoregulation. However, a possible role for PIEZO1 in kidney cystogenesis remains undefined. We hypothesized that cystogenesis in ADPKD reflects altered mechanotransduction, suggesting activation of mechanosensitive cation channels as a therapeutic strategy for ADPKD. Here, we show that Yoda-1 activation of PIEZO1 increases intracellular Ca2+and reduces forskolin-induced cAMP levels in mIMCD3 cells. Yoda-1 reduced forskolin-induced IMCD cyst surface areain vitroand in mouse metanephrosex vivoin a dose-dependent manner. Knockout of polycystin-2 dampened the efficacy of PIEZO1 activation in reducing both cAMP levels and cyst surface area in IMCD3 cells. However, collecting duct-specificPiezo1knockout neither induced cystogenesis in wild-type mice nor affected cystogenesis in thePkd1RC/RCmodel of ADPKD. Our study suggests that polycystin-2 and PIEZO1 play a role in mechanotransduction during cystogenesisin vitro, andex vivo, but thatin vivocyst expansion may require inactivation or repression of additional suppressors of cystogenesis and/or growth. Our study provides a preliminary proof of concept for PIEZO1 activation as a possible component of combination chemotherapy to retard or halt cystogenesis and/or cyst growth.
Effect of a constant magnetic field on morphology and motility of cell with cytoskeleton-associated magnetic nanoparticles
Authors: Karavashkova Olga , Maltseva Alexandra , Minin Artem S , Demin Alexander , Tin Pavel , Aitova Aleria , Tsvelaya Valeria , Latypova Anastasiia Alfredovna , Zubarev Ilya 
Published: May 2024

1.AbstractCell motility, shape supporting, and intracellular signaling are followed by changes in cell morphology and cytoskeleton. The cell reaction and the reorganization of the cytoskeleton occurs in a single volume of the cytoplasm and affects all components of the cytoskeleton: intermediate filaments, microtubules and microfilaments. A promising way to manipulate cells is magnetic nanoparticles that control cellular physiology. This approach is called magnetogenetics and has found application in various fields of cell and molecular biology. Using a magnetic field, it is possible to non-invasively regulate biochemical processes, migration and changes in the morphology of cells with magnetic nanoparticles. Our work opens up new possibilities for spatial manipulation of individual cytoskeletal components in vitro and operates biochemical pathways associated with individual cytoskeletal components.
Parallel feedback processing for voluntary control: knowledge of spinal feedback in motor cortex
Authors: Guang Hui , Nashed Joseph Y. , Pruszynski J. Andrew , Kalidindi Hari Teja , Crevecoeur Frederic , Cross Kevin P. , Blohm Gunnar , Scott Stephen H. 
Published: May 2024

AbstractHow hierarchical feedback pathways coordinate goal-directed motor corrections remains unknown. We show that systematic increases in spinal reflex responses to applied loads lead to a reciprocal reduction in the neural response in primary motor cortex. We use a neural network model to highlight how knowledge on lower-level feedback processing (i.e. spinal reflexes) can be embedded into a network to generate goal-directed corrections through parallel feedback pathways.
Temporal prediction captures key differences between spiking excitatory and inhibitory V1 neurons
Authors: Taylor Luke , Zenke Friedemann , King Andrew J. , Harper Nicol S. 
Published: May 2024

AbstractNeurons in primary visual cortex (V1) respond to natural scenes with a sparse and irregular spike code that is carefully balanced by an interplay between excitatory and inhibitory neurons. These neuron classes differ in their spike statistics, tuning preferences, connectivity statistics and temporal dynamics. To date, no single computational principle has been able to account for these properties. We developed a recurrently connected spiking network of excitatory and inhibitory units trained for efficient temporal prediction of natural movie clips. We found that the model exhibited simple and complex cell-like tuning, V1-like spike statistics, and, notably, also captured key differences between excitatory and inhibitory V1 neurons. This suggests that these properties collectively serve to facilitate efficient prediction of the sensory future.
Molecular and clinical characterization of a founder mutation causing G6PC3 deficiency
Authors: Zhen Xin , Betti Michael J , Kars Meltem Ece , Patterson Andrew , Medina-Torres Edgar Alejandro , Mendoza Selma Cecilia Scheffler , Sánchez Diana Andrea Herrera , Lopez-Herrera Gabriela , Svyryd Yevgeniya , Mutchinick Osvaldo M. , Gamazon Eric , Rathmell Jeffrey C , Itan Yuval , Markle Janet , Romanillos Patricia O’Farrill , Lugo-Reyes Saul Oswaldo , Martinez-Barricarte Ruben 
Published: May 2024

ABSTRACTBackgroundG6PC3 deficiency is a rare genetic disorder that causes syndromic congenital neutropenia. It is driven by the intracellular accumulation of a metabolite named 1,5-anhydroglucitol-6-phosphate (1,5-AG6P) that inhibits glycolysis. Patients display heterogeneous extra-hematological manifestations, contributing to delayed diagnosis.ObjectiveTheG6PC3c.210delC variant has been identified in patients of Mexican origin. We set out to study the origin and functional consequence of this mutation. Furthermore, we sought to characterize the clinical phenotypes caused by it.MethodsUsing whole-genome sequencing data, we conducted haplotype analysis to estimate the age of this allele and traced its ancestral origin. We examined how this mutation affected G6PC3 protein expression and performed extracellular flux assays on patient-derived cells to characterize how this mutation impacts glycolysis. Finally, we compared the clinical presentations of patients with the c.210delC mutation relative to other G6PC3 deficient patients published to date.ResultsBased on the length of haplotypes shared amongst ten carriers of theG6PC3c.210delC mutation, we estimated that this variant originated in a common ancestor of indigenous American origin. The mutation causes a frameshift that introduces a premature stop codon, leading to a complete loss of G6PC3 protein expression. When treated with 1,5-anhydroglucitol (1,5-AG), the precursor to 1,5-AG6P, patient-derived cells exhibited markedly reduced engagement of glycolysis. Clinically, c.210delC carriers display all the clinical features of syndromic severe congenital neutropenia type 4 observed in prior reports of G6PC3 deficiency.ConclusionTheG6PC3c.210delC is a loss-of-function mutation that arose from a founder effect in the indigenous Mexican population. These findings may facilitate the diagnosis of additional patients in this geographical area. Moreover, thein vitro1,5-AG-dependent functional assay used in our study could be employed to assess the pathogenicity of additionalG6PC3variants.
Small molecule biomarkers predictive of Chagas disease progression
Authors: Liu Zongyuan , Vucetich Steffany , DeToy Kelly , Saucedo Gustavo Durán , Verastegui Manuela , Carballo-Jimenez Paula , Mercado-Saavedra Brandon N. , Tinajeros Freddy , Málaga-Machaca Edith S. , Marcus Rachel , Gilman Robert H. , Bowman Natalie M. , McCall Laura-Isobel 
Published: May 2024

AbstractChagas disease (CD) is a neglected tropical disease caused by the parasitic protozoanTrypanosoma cruzi. However, only 20% to 30% of infected individuals will progress to severe symptomatic cardiac manifestations. Current treatments are benznidazole and nifurtimox, which are poorly tolerated regimens. Developing a biomarker to determine the likelihood of patient progression would be helpful for doctors to optimize patient treatment strategies. Such a biomarker would also benefit drug discovery efforts and clinical trials. In this study, we combined untargeted and targeted metabolomics to compare serum samples fromT. cruzi-infected individuals who progressed to severe cardiac disease, versus infected individuals who remained at the same disease stage (non-progressors). We identified four unannotated biomarker candidates, which were validated in an independent cohort using both untargeted and targeted analysis techniques. Overall, our findings demonstrate that serum small molecules can predict CD progression, offering potential for clinical monitoring.
Outcomes and Risk Factors for Postoperative Vasoplegia Following Implant of Continuous-Flow Left Ventricular Assist Devices
Authors: Wright Jonathan C. , Wilbur Jameson G. , Modi Sujal , Abdel-Rasoul Mahmoud , Whitson Bryan A. , Ganapathi Asvin M. , Smith Sakima 
Published: May 2024

AbstractRefractory postoperative vasoplegia following cardiac surgery and left ventricular assist device (LVAD) implantation predicts poor outcomes. We aimed to investigate outcomes and predictors of postoperative vasoplegia. We retrospectively reviewed a single-center cohort of 190 patients who received LVADs from January 2015 through March 2022 at The Ohio State Wexner Medical Center. Primary outcomes included duration of ICU stay, development of right heart failure (RHF), and mortality. Secondary outcomes included pre-implant medications and post-implant blood products. Vasoplegia was defined as physician documentation of vasoplegia with patients requiring ≥1 intravenous vasopressors within 48-hours following LVAD implantation to maintain a mean arterial pressure >65 mmHg for >24 hours. Overall, 55 (29%) patients developed vasoplegia following LVAD implantation. Our sample was stratified into two cohorts: patients with vasoplegia and without vasoplegia. Baseline characteristics, LVAD indication, creatinine clearance, and comorbidities did not vary significantly between cohorts. Patients without vasoplegia were more likely to have prior implantable cardiac defibrillators (p = 0.02) and were more commonly prescribed pre-operative inotropes (p = 0.03). Blood-product administration within the first 48-hours postoperatively did not differ. While patients with vasoplegia required a longer ICU stay post-op (5 vs 7 days, p = 0.03), there were no significant differences in development of RHF (39% vs 44%), 30-day (7% vs 13%), 1-year (31% vs 29%), and 2-year mortality (39% vs 33%) in patients without and with vasoplegia, respectively. Overall, our vasoplegia rates were consistent with previous literature (29%) and vasoplegia did not confer inferior outcomes for mortality or developing RHF.
Cell-matrix force transmission regulates the loss of naïve pluripotency in mouse embryonic stem cells
Authors: Viswanadha Srivatsava , Gómez-González Manuel , Labouesse Celine , Venturini Valeria , Trepat Xavier , Chalut Kevin , Kechagia Zanetta , Roca-Cusachs Pere 
Published: May 2024

AbstractThe first step in the differentiation of mouse embryonic stem cells (mESCs) is the loss of naïve pluripotency. This step involves a major reshaping of cells from a rounded to a spread, adhesive phenotype. Whereas such reshaping is typically associated with increased force transmission to the extracellular matrix (ECM), the magnitude and role of cell-matrix forces in the loss of naïve pluripotency is unknown. Here, we show that cell-matrix forces increase during, and are required for, the loss of naïve pluripotency in mESCs. Using traction force microscopy, we show that mESCs progressively increase cell-traction forces and mechanotransduction markers as naïve pluripotency dissolves. Modulation of force transmission through myosin inhibition, substrate stiffness, or spatial differences within mESC colonies regulates the process. Increased force is triggered by GSK3-mediated signalling, and the effect of force is regulated in part by its transmission to the cell nucleus. Our work unveils a major role of cell-ECM forces in pluripotency dissolution, adding an important aspect to the interplay between biochemical and biophysical cues that drive this process.
Novel cell states arise in embryonic cells devoid of key reprogramming factors
Authors: Youlten Scott E. , Miao Liyun , Hoppe Caroline , Boswell Curtis W. , Musaev Damir , Abdelmessih Mario , Krishnaswamy Smita , Tornini Valerie A. , Giraldez Antonio J. 
Published: May 2024

SummaryThe capacity for embryonic cells to differentiate relies on a large-scale reprogramming of the oocyte and sperm nucleus into a transient totipotent state. In zebrafish, this reprogramming step is achieved by the pioneer factors Nanog, Pou5f3, and Sox19b (NPS). Yet, it remains unclear whether cells lacking this reprogramming step are directed towards wild type states or towards novel developmental canals in the Waddington landscape of embryonic development. Here we investigate the developmental fate of embryonic cells mutant for NPS by analyzing their single-cell gene expression profiles. We find that cells lacking the first developmental reprogramming steps can acquire distinct cell states. These states are manifested by gene expression modules that result from a failure of nuclear reprogramming, the persistence of the maternal program, and the activation of somatic compensatory programs. As a result, most mutant cells follow new developmental canals and acquire new mixed cell states in development. In contrast, a group of mutant cells acquire primordial germ cell-like states, suggesting that NPS-dependent reprogramming is dispensable for these cell states. Together, these results demonstrate that developmental reprogramming after fertilization is required to differentiate most canonical developmental programs, and loss of the transient totipotent state canalizes embryonic cells into new developmental statesin vivo.
A computational model for angular velocity integration in a locust heading circuit
Authors: Pabst Kathrin , Gkanias Evripidis , Webb Barbara , Homberg Uwe , Endres Dominik 
Published: May 2024

Accurate navigation often requires the maintenance of a robust internal estimate of heading relative to external surroundings. We propose a novel model for angular velocity integration to update the representation of heading in the central complex of the desert locust. In contrast to similar models proposed for the fruit fly, this circuit model uses a single 360° heading direction representation and is updated by neuromodulatory angular velocity inputs. Our computational model was implemented using steady-state firing rate neurons with dynamical synapses. The circuit connectivity was constrained by biological data and remaining degrees of freedom were optimised with a machine learning approach to yield physiologically plausible neuron activities. We demonstrate that the integration of heading and angular velocity in this circuit is robust to noise. The heading signal can be effectively used as input to an existing insect goal-directed steering circuit, adapted for outbound locomotion in a steady direction that resembles locust migration. Our study supports the possibility that similar computations for orientation may be implemented differently in the neural hardware of the fruit fly and the locust.