bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytoplasmic overexpression with membrane accentuation of stratifin in intrahepatic mass‐forming cholangiocarcinoma after hepatectomy: Correlation with clinicopathologic features and patient survival
Authors: Yeh Chun‐Nan , Pang See‐Tong , Jung Shih‐Ming , Chen Tsung‐Wen , Jan Yi‐Yin , Chen Miin‐Fu 
Published: October 2010

AbstractPurposeCholangiocarcinoma (CCA) is a lethal malignancy that afflicts thousands of patients worldwide. Stratifin has been shown to participate in mediating G2 arrest in several cancers, and cells that express stratifin could contribute to the chemo‐ and radioresistance of cancers and poor prognosis. However, the clinical impact of stratifin on clinicopathological features of mass forming (MF)‐CCA is still unclear.MethodsSeventy‐eight patients with MF‐CCA who had undergone hepatectomy were selected for an immunohistochemical study of stratifin. Sixteen clinicopathological variables were used for the survival analyses.ResultsSeventy‐eight MF‐CCA patients (36 men and 42 women) were studied. Cytoplasmic immunostaining with membrane prominence was found in 52.6% (41/78) of patients with MF‐CCA after hepatectomy; this was significantly associated with elevated carcinoembryonic antigen (CEA) levels. During the median follow‐up duration of 13.6 months, the 5‐year overall survival (OS) rate was 14.9%. Univariate analysis showed that an absence of clinical symptoms, better nutritional status, lower alkaline phosphatase, smaller tumor, negative lymph node metastasis, negative stratifin staining, and curative hepatic resection were associated with favorable OS rate for MF‐CCA patients after hepatectomy. Multivariate Cox proportional hazard analysis showed that the absence of clinical symptoms, negative lymph node metastasis, and curative hepatectomy independently predicted MF‐CCA patients with favorable OS rate after hepatectomy.ConclusionsOverexpression of stratifin was significantly associated with elevated CEA levels in patients with MF‐CCA. The favorable OS for MF‐CCA patients depends on the absence of clinical symptoms, negative lymph node metastasis, and curative hepatectomy. J. Surg. Oncol. 2010;102:608–614. © 2010 Wiley‐Liss, Inc.
Surgical management in metastatic gastrointestinal stromal tumor (GIST) patients after imatinib mesylate treatment
Authors: Yeh Chun‐Nan , Chen Tsung‐Wen , Tseng Jeng‐Hwei , Liu Yu‐Yin , Wang Shang‐Yu , Tsai Chun‐Yi , Chiang Kun‐Chun , Hwang Tsann‐Long , Jan Yi‐Yin , Chen Miin‐Fu 
Published: October 2010

AbstractPurposeImatinib mesylate (IM) demonstrates substantial efficacy in most patients with metastatic gastrointestinal stromal tumors (GISTs). However, progression of GIST eventually develops and emerges as a challenge. To assess the role of surgery in the multidisciplinary management of GISTs, we studied the surgical outcomes in GIST patients receiving IM.Materials and MethodsBetween 2001 and May 2009, 161 metastatic GIST patients received IM. Among them, 35 patients undergoing 38 surgeries were investigated. Patients were categorized based on extent of disease before surgery (responsive or stable disease (PR, SD), local progression (LP), and generalized progression (GP)). Each tumor was investigated for genetic alteration before and after surgery.ResultsDisease status before surgery was significantly associated with surgical result. Gross tumor clearance was achieved in 42.9% of patients with responsive disease, but only 4.8% of those with focal resistance and 0% of those with disease progression (P = 0.022). GIST patients with PR, SD, and LP had significant better 2‐year progression‐free survival and overall survival than those with GP. Secondary mutations tended to be found more frequently in GIST patients with LP after surgery than those with response (10/21 (47.6%) vs. 2/14 (14.3%); P = 0.07), indicating that surgery may prevent potential development of secondary mutation in GIST patients with response. Secondary kit mutations were also found more frequently with primary exon 11 mutation than those with exon 9 mutation (38.7% vs. 16.7%; P = 0.394).ConclusionsSurgery may benefit selected GIST patients with PR, SD, and LP, especially for patients with LP because patients with LP had comparable survival to that of patients with responsive lesion. Surgery may prevent potential development of secondary mutations in selected patients with response after IM treatment. Secondary kit mutation was found more frequently in GIST patients with a primary kit exon 11 mutation than those with a primary kit exon 9 mutation. J. Surg. Oncol. 2010;102:599–603. © 2010 Wiley‐Liss, Inc.
The extent of lymph node dissection for gastric cancer: A critical appraisal
Authors: de Bree Eelco , Charalampakis Vasilis , Melissas John , Tsiftsis Dimitris D. 
Published: October 2010

AbstractThe extent of lymphadenectomy during therapeutic gastrectomy for gastric cancer remains a protracted and controversial issue. While traditionally extended lymphadenectomy is performed in Eastern Asia, limited lymphadenectomy is advocated by most western surgeons. Two large western randomized trials, meta‐analyses and a recent systematic review were unable to demonstrate overall benefit from extended lymphadenectomy. In this review, the currently available data on this topic are critically evaluated, while ongoing studies and future perspective are discussed. J. Surg. Oncol. 2010;102:552–562. © 2010 Wiley‐Liss, Inc.
Florid cutaneous papillomatosis
Authors: Janniger Edmund J. , Schwartz Robert A. 
Published: October 2010

AbstractFlorid cutaneous papillomatosis is an obligate paraneoplastic syndrome. Originally described by Schwartz and Burgess, and also known as the Schwartz–Burgess syndrome, it is a widespread eruption resembling viral warts yet with no evidence of human papilloma virus infection. We review this entity and its implications. J. Surg. Oncol. 2010;102:709–712. © 2010 Wiley‐Liss, Inc.
Novel influenza a (H1N1) viral infection in pediatric patients with sickle‐cell disease
Authors: Jacobs James E. , Quirolo Keith , Vichinsky Elliott 
Published: October 2010

AbstractBackgroundThe 2009 novel influenza A (H1N1) pandemic has had profound public health implications all over the world. The majority of patients infected with the novel strain have recovered uneventfully. However, certain populations have been defined who appear to be at increased risk of complications due to H1N1 infections. This review summarizes the clinical course of five patients with sickle cell, four of whom had confirmed H1N1 infection, and one whom had a presumed H1N1 infection.ProcedureThe clinical presentation, hospital course, and treatment of five pediatric patients with sickle‐cell disease and H1N1 infection were reviewed retrospectively.ResultsIn this case series, our patients experienced complications such as the acute chest syndrome, acute marrow suppression of red cell production, pain crisis, and hematuria.ConclusionsIn this population, who are at increased risk for bacterial superinfection as well as complications from the influenza virus itself, vigilance toward diagnosis and aggressive treatment will continue to be important as long as the novel virus is in circulation. Pediatr Blood Cancer. 2010;56:95–98. © 2010 Wiley‐Liss, Inc.
TEL/AML1‐positive patients lacking TEL exon 5 resemble canonical TEL/AML1 cases
Authors: Zaliova Marketa , Meyer Claus , Cario Gunnar , Vaskova Martina , Marschalek Rolf , Stary Jan , Zuna Jan , Trka Jan 
Published: October 2010

AbstractBackgroundThe TEL/AML1 fusion gene which represents the most frequent genetic abnormality in childhood ALL, usually results from genomic breakpoints in TEL intron 5 and AML1 intron 1 or 2. At the protein level, the helix–loop–helix domain and exon 5‐coded central region of TEL are typically fused to almost entire AML1 including DNA‐binding domain.ProcedureWe identified two ALL patients with genomic breakpoints within TEL intron 4 resulting in variant TEL/AML1 fusion lacking the TEL exon 5‐coded central region. This region was supposed to play an important role in TEL/AML1 function, particularly in TEL/AML1‐mediated transcriptional repression of AML1 targets. We aimed at investigating the impact of the loss of this region on disease behavior and TEL/AML1 function. We compared clinical and biological characteristics, treatment response, and outcome of the variant versus classical TEL/AML1 cases, analyzed genome wide gene expression profiles and performed reporter gene assay.ResultsNo distinct differences between variant and classical TEL/AML1 cases were observed including gene expression profiling and detailed immunophenotyping. By using reporter gene assay, we showed that the loss of the central region does not influence the TEL/AML1‐mediated transcriptional repression.ConclusionsThe deletion of the central region did not affect the TEL/AML1‐specific phenotype; we did not find any relevant differences in clinical and biological features when variant versus classical TEL/AML1‐positive cases were compared. Thus, our data does not support hypothesis that the central region of TEL is indispensable for TEL/AML1 driven leukemogenesis. Pediatr Blood Cancer 2011;56:217–225. © 2010 Wiley‐Liss, Inc.
2009 pandemic influenza a (H1N1) virus infection in pediatric oncology and hematopoietic stem cell transplantation patients
Authors: Cost Carrye , Brock Evangeline , Adams‐Huet Beverley , Siegel Jane D. , Ardura Monica I. 
Published: October 2010

AbstractBackgroundPediatric oncology and hematopoietic stem cell transplantation (HSCT) patients are at high risk for influenza infection and its associated complications. Little is known about infection with novel 2009 influenza A (H1N1) in this population.ProcedureProspective laboratory surveillance identified all children with positive influenza test results from 4/27/09–12/5/09. 2009 H1N1 infection was confirmed by PCR subtyping; cases in which subtyping was not performed were considered probable. Medical records of all pediatric oncology and HSCT cases were reviewed.ResultsThirty children with cancer or HSCT had laboratory‐confirmed influenza A. Patients with ALL (18), CNS tumors (4), CML (1), Ewing sarcoma (1), Hodgkin lymphoma (1), LCH (1), severe aplastic anemia (1), and HSCT (3), had confirmed (5) and probable (25) H1N1 by rapid (22; 73%), DFA (4; 13%), or RVP (4; 13%) assays. Most frequent presenting signs and symptoms were fever (93%; median 38.6°C), cough (97%), and rhinorrhea (83%). Ten patients required hospitalization for a median of 5 days, most commonly for fever and neutropenia (8). Imaging demonstrated lower respiratory tract involvement in three patients. There were no concomitant bacteremias; one patient had rhinovirus co‐infection. Three patients required ICU care; 1 developed ARDS, multi‐organ failure, and died after 5 days. Chemotherapy was delayed in five patients. Oseltamivir was administered to 28 patients; 1 patient developed an oseltamivir‐resistant strain and was treated with zanamivir.Conclusions2009 influenza A H1N1 infection in children with cancer and HSCT is mild in most patients, but can lead to serious complications. Pediatr Blood Cancer. 2010;56:127–133. © 2010 Wiley‐Liss, Inc.
Outcome of second allogenic stem cell transplantation in pediatric patients with non‐malignant hematological and immune deficiency disorders
Authors: Ayas Mouhab , Al‐Jefri Abdullah , Eldali Abdelmoneim , Al‐Seraihi Amal , Al‐Mahr Mohammed , Al‐Ghonaium Abdulaziz , Al‐Ahmari Ali , Al‐Muhsen Saleh , Al‐Mousa Hamoud , Al‐Dhekri Hasan , Al‐Saud Bandar , El‐Solh Hassan 
Published: October 2010

AbstractBackgroundSecond stem cell transplantation (SCT) is usually associated with high morbidity and mortality and the data on its outcome in pediatric patients with non‐malignant disorders are scarce.Patients and MethodsWe present 30 children with non‐malignant conditions who underwent second SCT at our institution for graft failure after the first SCT; 20 had a non‐malignant hematological disorder and 10 had an immune deficiency disorder. Median age at the second SCT was 6.1 years (range, 0.4–13 years) and median time from the first SCT to the second SCT was 6.2 months (range, 1.2–96 months).ResultsTwenty patients (70%) engrafted; severe acute GVHD developed in four patients (13%), and chronic GVHD developed in two patients of those at risk (10%). Thirteen deaths occurred and nine were considered treatment related. The 5‐year overall (OS) and event free survival (EFS) for all patients were 53% and 47% respectively. The interval between the two transplants seemed to affect the outcome; patients who had the second SCT ≥6 months from the first SCT had better survival; the 5‐year OS for the two groups (<6 months and ≥6 months) respectively were 30% and 74% (P = 0.004), and the 5‐year EFS were 27% and 66% (P = 0.004). The underlying disease did not affect the outcome nor did the use of radiation in the conditioning regimen for the second SCT.ConclusionsSecond SCT for graft failure should be considered for children with non‐malignant hematological and immune deficiency disorders. Pediatr Blood Cancer 2011;56:289–293. © 2010 Wiley‐Liss, Inc.
Simultaneous tumors: Acute myeloid leukemia infiltrating mediastinal ganglioneuroblastoma
Authors: Di Cataldo Andrea , Mazzocco Katia , Magro Gaetano , Mirabile Elena , Nigro Luca Lo , Defferrari Raffaella , Tonini Gian Paolo 
Published: October 2010

AbstractWe report the case of a child presenting with concurrent thoracic ganglioneuroblastoma and acute myeloid leukemia. The peculiarity was the close relation between the two tumors with the latter infiltrating the former one. Histological and genomic studies indicate the different clonal origins of the malignancies in the patient, and we hypothesized that GNB and AML developed independently. Our observation suggests that in patients with more than one tumor, though discovered at different times, one neoplasm is not always secondary. Pediatr Blood Cancer 2011;56:298–300. © 2010 Wiley‐Liss, Inc.
Pediatric histiocytic sarcoma clonally related to precursor B‐cell acute lymphoblastic leukemia with homozygous deletion of CDKN2A encoding p16INK4A
Authors: Kumar Riten , Khan Shakila P. , Joshi Divya‐Devi , Shaw Gene R. , Ketterling Rhett P. , Feldman Andrew L. 
Published: October 2010

AbstractHistiocytic sarcoma (HS) is a rare malignancy of tissue histiocytes with a dismal prognosis. We report a 4‐year‐old male who developed HS during maintenance chemotherapy for precursor B‐cell acute lymphoblastic leukemia (pre‐B ALL). Both tumors showed identical clonal immunoglobulin and T‐cell receptor gene re‐arrangement patterns, as well as homozygous deletion of the CDKN2A gene encoding p16INK4A. These data suggest a clonal relationship between the two neoplasms despite their distinct lineages. Since CDKN2A deletion predisposes to development of HS in experimental models, the cytogenetic features of the patient's pre‐B ALL may have predisposed to this change in lineage. Pediatr Blood Cancer 2011;56:307–310. © 2010 Wiley‐Liss, Inc.
A “self‐pinning” adhesive based on responsive surface wrinkles
Authors: Chan Edwin P. , Karp Jeffrey M. , Langer Robert S. 
Published: October 2010

AbstractSurface wrinkles are interesting since they form spontaneously into well‐defined patterns. The mechanism of formation is well‐studied and is associated with the development of a critical compressive stress that induces the elastic instability. In this work, we demonstrate surface wrinkles that dynamically change in response to a stimulus can improve interfacial adhesion with a hydrogel surface through the dynamic evolution of the wrinkle morphology. We observe that this control is related to the local pinning of the crack separation pathway facilitated by the surface wrinkles during debonding, which is dependent on the contact time with the hydrogel. © 2010 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys, 2010
Characterization of sugar juice heat exchanger tube deposit
Authors: East C. P. , Doherty W. O. S. , Fellows C. M. , Yu H. 
Published: October 2010

AbstractFouling of heat exchanger surfaces during sugar manufacture reduces productivity and increases energy demand. This study characterizes a deposit (including its internal structure) formed in a sugar factory's evaporator unit using a variety of X‐ray diffraction and scanning electron microscopy techniques (including X‐ray powder diffraction, X‐ray fluorescence, elemental mapping with energy dispersive spectroscopy and backscattered electron imaging). Calcium sulfate dihydrate, calcium oxalate mono‐ and dihydrate, hydroxyapatite, amorphous silica and organic matter are present in the deposit. The composition of the deposit (which contains three layers) varies along the height of the tube. There are noticeable differences in the composition and porosity among the layers in the deposit. A porous structure consisting of a mixture of amorphous silica, calcium oxalate dihydrate and organic matter is attached to the surface of the deposit in contact with sugar juice, while a denser morphology of amorphous silica and hydroxyapatite is attached to the tube wall. Elemental mapping identifies an association between Si, Al, Mg, Fe and O, suggesting the presence of a silicate compound as a minor component in the deposit. An attempt is made to rationalize the formation of the observed phases. Copyright © 2010 John Wiley & Sons, Ltd.
Electrical, structural and morphological characteristics of rapidly annealed Ni/Pd Schottky rectifiers on n‐type GaN
Authors: Siva Pratap Reddy M. , Rajagopal Reddy V. , Jyothi I. , Choi Chel‐Jong 
Published: October 2010

AbstractSchottky rectifiers are fabricated on n‐type GaN using Ni/Pd metallization scheme and its characteristics have been investigated by current‐voltage (I‐V), Capacitance‐Voltage (C‐V), X‐Ray Diffraction (XRD) and SIMS measurements as a function of annealing temperature. The calculated Schottky barrier height of the as‐deposited contact was found to be 0.60 eV (I‐V), 0.71 eV (C‐V) with an ideality factor of 1.44. However, the barrier height slightly increases after annealing at 300, 400 and 500 °C. On the basis of the experimental results, a high‐quality Schottky contact with barrier height and ideality factor of 0.81 eV (I‐V), 0.88 eV (C‐V) and 1.13 respectively, can be obtained after annealing at 600 °C for 1 min in a nitrogen atmosphere. Further, after annealing at 700 °C, it is found that the barrier height slightly decreased to 0.74 eV (I‐V) and 0.85 eV (C‐V). From the above observations, one can note that Ni/Pd Schottky contact exhibits excellent electrical properties after a rapid thermal annealing at 600 °C. According to the SIMS and XRD analysis, the formation of gallide phases at the Ni/Pd/n‐GaN interface could be the reason of the barrier height increase at elevated annealing temperatures. The Atomic Force Microscopy (AFM) results show that the overall surface morphology of Ni/Pd Schottky contacts on n‐GaN is fairly smooth. The above observations reveal that Ni/Pd Schottky metallization scheme was a good choice for the fabrication of high‐temperature and high‐power device applications. Copyright © 2010 John Wiley & Sons, Ltd.
SIMS depth profiling of semiconductor interfaces: Experimental study of depth resolution function
Authors: Kudriavtsev Yu. , Gallardo S. , Koudriavtseva O. , Escobosa A. , Sanchez–R V. M. , Avendaño M. , Asomoza R. , Lopez‐Lopez M. 
Published: October 2010

AbstractReconstruction of original element distribution at semiconductor interfaces using experimental SIMS profiles encounters considerable difficulties because of the matrix effect, sputtering rate change at the interface, and also a sputtering‐induced broadening of original distributions. We performed a detailed depth profiling analysis of the Al step‐function distribution in GaAs/AlxGa1−xAs heterostructures by using Cs+ primary ion beam sputtering and CsM+ cluster ion monitoring (where M is the element of interest) to suppress the matrix effect. The experimental Depth Resolution Function (DRF) was obtained by differentiation of the Al step‐function profile and compared with the ‘reference’ DRF found from depth profiling of an Al delta layer. The difference between two experimental DRFs was explained by the sputtering rate change during the interface profiling. We experimentally studied the sputtering rate dependence on the AlxGa1−xAs layer composition and applied it for a reconstruction of the DRF found by differentiating the Al step‐function distribution: the ‘reconstructed’ and ‘reference’ DRFs were found to be in good agreement. This confirmed the correctness of the treatment elaborated. Copyright © 2010 John Wiley & Sons, Ltd.
Efficient and rapid exact gene replacement without selection
Authors: Cross Frederick R. , Pecani Kresti 
Published: October 2010

AbstractWe describe a highly efficient method for exact gene replacement in budding yeast. Induction of rapid and efficient recombination in an entire cell population results in at least 50% of the recombinants undergoing a switch of the endogenous copy to a specific mutated allele, with no remaining markers or remnant of foreign DNA, without selection. To accomplish this, a partial copy of the replacement allele, followed by an HO cut site, is installed adjacent to the wild‐type locus, in a GAL‐HO MATa‐inc background. HO induction results in near‐quantitative site cleavage and recombination/gene conversion, resulting in either regeneration of wild‐type or switch of the endogenous allele to the mutant, with accompanying deletion of intervening marker sequences, yielding an exact replacement. Eliminating the need for selection (over days) of rare recombinants removes concerns about second‐site suppressor mutations and also allows direct phenotypic analysis, even of lethal gene replacements, without the need of a method to make the lethality conditional or to employ regulated promoters of unknown strength compared to the endogenous promoter. To test this method, we tried two known lethal gene replacements, substituting the non‐essential CDH1 gene with a dominantly lethal version mutated for its Cdk phosphorylation sites and substituting the essential CDC28 gene with two recessively lethal versions, one containing an early stop codon and another inactivating Cdc28 kinase activity. We also tested a gene replacement of unknown phenotypic consequences: replacing the non‐essential CLB3 B‐type cyclin with a version lacking its destruction box. Copyright © 2010 John Wiley & Sons, Ltd.
Synthesis and Self‐Assembly Behavior of Comb‐Like Surfactant Polymethyl Methacrylate‐g‐Methoxy Polyethylene Glycol
Authors: Sun Lian‐lian , Du Zhi‐ping , Wang Wan‐xu , Liu Ying 
Published: October 2010

AbstractComb‐like surfactant of polymethyl methacrylate‐g‐methoxy polyethylene glycol (PMMA‐g‐MPEG) with well‐defined structure was synthesized and characterized by FT‐IR, 1H NMR and gel permeation chromatography. Its surface activity and aggregation behavior in aqueous solution were investigated by surface tension measurement, dye encapsulation experiment, transmission electron microscopy (TEM) and dynamic light scattering (DLS). The CMC and surface tension at the CMC of PMMA‐g‐MPEG were 0.2 g/L and 47 mN/m, respectively. DLS and TEM analysis revealed that PMMA‐g‐MPEG could self‐assemble into spherical micelles with the average hydrodynamic diameters in the range from 20 to 300 nm in aqueous solution.