bluuebunny/crossref_metadata_2025_split huggingface dataset:
Altered counts and mitochondrial mass of peripheral blood leucocytes in patients with chronic hepatitis B virus infection
Authors: Zhou Ruo‐Ran , Song Ya‐Hui , Xu Cheng‐Yu , Zhang Ying‐Ying , Wu Xiang‐Wei , Zhang Lu , Luo Xi‐Ni , Zhao Han , Liu Ming‐Ming , Xu Jun‐Chi , Wang Lin , Chen Zu‐Tao , Han Qing‐Zhen 
Published: June 2024

AbstractHepatitis B virus (HBV) damages liver cells through abnormal immune responses. Mitochondrial metabolism is necessary for effector functions of white blood cells (WBCs). The aim was to investigate the altered counts and mitochondrial mass (MM) of WBCs by two novel indicators of mitochondrial mass, MM and percentage of low mitochondrial membrane potential, MMPlow%, due to chronic HBV infection. The counts of lymphocytes, neutrophils and monocytes in the HBV infection group were in decline, especially for lymphocyte (p = 0.034) and monocyte counts (p = 0.003). The degraded MM (p = 0.003) and MMPlow% (p = 0.002) of lymphocytes and MM (p = 0.005) of monocytes suggested mitochondrial dysfunction of WBCs. HBV DNA within WBCs showed an extensive effect on mitochondria metabolic potential of lymphocytes, neutrophils and monocytes indicated by MM; hepatitis B e antigen was associated with instant mitochondrial energy supply indicated by MMPlow% of neutrophils; hepatitis B surface antigen, antiviral therapy by nucleos(t)ide analogues and prolonged infection were also vital factors contributing to WBC alterations. Moreover, degraded neutrophils and monocytes could be used to monitor immune responses reflecting chronic liver fibrosis and inflammatory damage. In conclusion, MM combined with cell counts of WBCs could profoundly reflect WBC alterations for monitoring chronic HBV infection. Moreover, HBV DNA within WBCs may be a vital factor in injuring mitochondria metabolic potential.
Whole‐genome analysis reveals phylogenetic and demographic history of Eurasian perch
Authors: Lichman Vitalii , Ozerov Mikhail , López María‐Eugenia , Noreikiene Kristina , Kahar Siim , Pukk Lilian , Burimski Oksana , Gross Riho , Vasemägi Anti 
Published: June 2024

AbstractThe contemporary diversity and distribution of species are shaped by their evolutionary and ecological history. This can be deciphered with the help of phylogenetic and demographic analysis methods, ideally combining and supplementing information from mitochondrial and nuclear genomes. In this study, we investigated the demographic history of Eurasian perch (Perca fluviatilis), a highly adaptable teleost with a distribution range across Eurasia. We combined whole‐genome resequencing data with available genomic resources to analyse the phylogeny, phylogeography, and demographic history of P. fluviatilis populations from Europe and Siberia. We identified five highly diverged evolutionary mtDNA lineages, three of which show a strong signal of admixture in the Baltic Sea region. The estimated mean divergence time between these lineages ranged from 0.24 to 1.42 million years. Based on nuclear genomes, two distinct demographic trajectories were observed in European and Siberian samples reflecting contrasting demographic histories ca. 30,000–100,000 years before the present. A comparison of mtDNA and nuclear DNA evolutionary trees and AMOVA revealed concordances, as well as incongruences, between the two types of data, most likely reflecting recent postglacial colonization and hybridization events. Overall, our findings demonstrate the power and usefulness of genome‐wide information for delineating historical processes that have shaped the genome of P. fluviatilis. We also highlight the added value of data‐mining existing transcriptomic resources to complement novel sequence data, helping to shed light on putative glacial refugia and postglacial recolonization routes.
Detection of AFB1 in corn by MXene paper‐based unlabeled aptasensor
Authors: Wang Chengquan , Gu Chengdong , Rong yanna , Zhao Xin , Qian Lu , Liu Mengting , Huang Xingyi , Qian Jing 
Published: June 2024

AbstractIn this work, a paper‐based electrochemical aptamer sensor was developed for the detection of aflatoxin B1 (AFB1) using a combination of MXene–Ti3C2Tx and nucleic acid aptamers. The prepared single‐layer or few‐layer MXene suspension is suction‐filtered onto MXene paper, which is cut to prepare MXene electrodes. To accomplish AFB1 specific detection, an amino‐labeled AFB1 aptamer is mounted on the surface of the carboxy‐functionalized MXene electrode. When AFB1 is present, it particularly binds to the aptamer to form a 3D structure, reducing the efficiency of electron transmission on the sensor surface. The difference in impedance signal change at the electrode/electrolyte interface is used to quantify AFB1. The results indicated that the detection range is 0.05–100 ng/mL, the detection limit is 0.04 ng/mL, and the recovery rate of AFB1 in corn samples is 97.8%–111.52% with the optimal detection conditions. The MXene paper‐based label‐free aptasensor is versatile and can detect different targets by simply swapping out the aptamers of different targets. The sensor also has a wide range of applications in food analysis and environmental testing.Practical applicationsA paper‐based electrochemical aptamer sensor was developed to detect aflatoxin B1 using a combination of MXene–Ti3C2Tx and nucleic acid aptamers.The design is based on the preparation of MXene electrodes by pumping and filtering monolayer or multilayer MXene suspensions onto MXene paper and cutting.The MXene paper‐based label‐free aptamer sensor was designed to be versatile, allowing the detection of different targets by simply replacing the aptamer with one from a different targets.
Advancements and 4E + Q performance analyses in solar drying for maize kernels preservation: A comprehensive review
Authors: Suherman Suherman , Asy‐Syaqiq Muhammad Anas , Hadiyanto Hadiyanto , Djaeni Mohamad , Prasetiyono Bambang Waluyo Hadi Eko 
Published: June 2024

AbstractThe world's population is projected to increase, impacting a 60% rise in food production demand by 2050, including the demand for maize. Currently, maize production is estimated to reach 1510 million tons in 2023, necessitating suitable methods for preserving maize kernels to maintain quality and reduce post‐harvest agricultural losses. Although drying methods present a viable solution to address this issue, the majority of farmers in Indonesia still use conventional drying methods, such as open sun drying, which has several drawbacks. Hence, solar drying systems aim to overcome these limitations by harnessing renewable, clean, sustainable, and eco‐friendly energy to support the sustainable development goals (SDGs). This research comprehensively reviews the progress of solar drying technology, providing insights into its working principles, innovative designs, and operational modes across various types of solar dryers. The 4E + Q analysis provides information regarding the performance of solar drying systems, including energy analysis to determine the quantity of energy utilized and exergy analysis to demonstrate irreversibility in thermodynamic processes. Additionally, this analysis highlights environmental impacts such as CO2 emissions and mitigation efforts, while considering economic aspects through lifecycle assessment and payback period methods. The quality assessment of dried maize kernels according to the standards set by the Indonesian National Standard (SNI) is conducted, including proximate analysis, aflatoxin levels, color measurements, and internal cracking analysis.Practical applicationsDrying technology is essential for the food preservation process, enabling the maintenance of product quality and extending storage life by reducing the moisture content to eliminate the activities of microbial agents. The application of solar drying technology harnesses solar radiation as the primary energy source, making it well‐suited for crops like paddy, maize, cassava, etc. The development of solar dryers continues through modifications and the addition of auxiliary systems to achieve more efficient, economical, and sustainable technologies. The hope is that in the future, these technologies can be adopted by farmers on an industrial scale due to low operational costs, ease of operation, and controllable operating conditions. The methodological approach is necessary to evaluate the performance of solar dryers and the quality of dried products through a 4E + Q analysis, it provides information on energy consumption, exergy flow, economic considerations, environmental aspects, and quality assessments against national quality standards (SNI).
Green approaches for biofilm eradication: Enhancing cleaning efficiency
Authors: Mechmechani Samah , Jha Piyush Kumar , Karam Layal , Dallagi Heni 
Published: June 2024

AbstractCleaning up biocontamination from surfaces is a critical aspect of maintaining a hygienic environment. Traditional cleaning methods often fall short when it comes to eliminating persistent biofilms and resilient bacteria. In recent years, alternative approaches utilizing antibiofilm enzymes, bacteriophages, essential oils (EOs), antibacterial peptides, and biosurfactants have emerged as promising strategies to combat biocontamination. This article explores the efficiency of these agents in targeting and eradicating biofilms, highlighting their mechanisms of action and potential applications. By leveraging the unique properties of these materials, we can enhance cleaning practices and improve public health outcomes by effectively eliminating biocontamination from various surfaces.
Antibiotics for delirium in older adults with pyuria or bacteriuria: A systematic review
Authors: Stall Nathan M. , Kandel Christopher , Reppas‐Rindlisbacher Christina , Quinn Kieran L. , Wiesenfeld Lesley , MacFadden Derek R. , Johnstone Jennie , Fralick Michael 
Published: June 2024

AbstractBackgroundIt is unclear whether antibiotics impact delirium outcomes in older adults with pyuria or bacteriuria in the absence of systemic signs of infection or genitourinary symptoms.MethodsWe registered our systematic review protocol with PROSPERO (CRD42023418091). We searched the Medline and Embase databases from inception until April 2023 for studies investigating the impact of antimicrobial treatment on the duration and severity of delirium in older adults (≥60 years) with pyuria (white blood cells detected on urinalysis or dipstick) or bacteriuria (bacteria growing on urine culture) and without systemic signs of infection (temperature > 37.9C [>100.2F] or 1.5C [2.4F] increase above baseline temperature, and/or hemodynamic instability) or genitourinary symptoms (acute dysuria or new/worsening urinary symptoms). Two reviewers independently screened search results, abstracted data, and appraised the risk of bias. Full‐text randomized controlled trials (RCTs) and observational study designs were included without restriction on study language, duration, or year of publication.ResultsWe screened 984 citations and included 4 studies comprising 652 older adults (mean age was 84.6 years and 63.5% were women). The four studies were published between 1996 and 2022, and included one RCT, two prospective observational cohort studies, and one retrospective chart review. None of the four studies demonstrated a significant effect of antibiotics on delirium outcomes, with two studies reported a worsening of outcomes among adults who received antibiotics. The three observational studies included had a moderate or serious overall risk of bias, while the one RCT had a high overall risk of bias.ConclusionsOur systematic review found no evidence that treatment with antibiotics is associated with improved delirium outcomes in older adults with pyuria or bacteriuria and without systemic signs of infection or genitourinary symptoms. Overall, the evidence was limited, largely observational, and had substantial risk of bias.
Physical pain among family caregivers to older adults: A scoping review of the literature
Authors: Turner Shelbie G. , Pillemer Karl , Demetres Michelle , Heaney Kathleen , Joshi Sama , Luebke Matthew , Messay Gelila , Reid M. Carrington 
Published: June 2024

AbstractBackground and ObjectivesScholarship on the health of family caregivers to older adults continues to expand. Although existing research suggests that many family caregivers experience pain, which impacts their ability to perform caregiving tasks and is associated with care recipients' unmet needs, the scope of research on family caregivers' pain remains poorly characterized. We conducted a scoping review of research on pain among family caregivers to older adults to characterize existing evidence and identify knowledge gaps.MethodsWe searched multiple databases spanning from January 2012 to July 2023, identified eligible studies using predefined inclusion/exclusion criteria, and extracted key data (e.g., study design/methodology, pain measurement, caregiver pain type, and major findings).ResultsWe identified 46 eligible studies conducted in the United States (n = 19) and internationally (n = 27). Studies often focused on caregivers for older adults with specific health conditions, such as cancer (n = 11), dementia (n = 8), or stroke (n = 3). The most commonly employed pain measure was a single‐item dichotomous question about pain (n = 16), followed by a visual numeric or visual analog scale (n = 11). Nine studies (five randomized controlled trials) reported on five caregiver pain management interventions, including yoga/exercise programs and caregiver education programs.DiscussionExisting research on family caregivers' pain offers an important foundation. However, more robust research designs are necessary. We identify possibilities for future studies in addition to opportunities for systematic investigations to support the family caregivers being relied upon to care for the increasing number of older adults.
DNA methylation controlling abscisic acid catabolism responds to light to mediate strawberry fruit ripening
Authors: Sun Yunfan , Yang Xiaofang , Wu Rongrong , Lv Shouzheng , Li Yunduan , Jia Haoran , Yang Yuying , Li Baijun , Chen Wenbo , Allan Andrew C. , Jiang Guihua , Shi Yan‐Na , Chen Kunsong 
Published: June 2024

AbstractPhytohormones, epigenetic regulation and environmental factors regulate fruit ripening but their interplay during strawberry fruit ripening remains to be determined. In this study, bagged strawberry fruit exhibited delayed ripening compared with fruit grown in normal light, correlating with reduced abscisic acid (ABA) accumulation. Transcription of the key ABA catabolism gene, ABA 8′‐hydroxylase FaCYP707A4, was induced in bagged fruit. With light exclusion whole genome DNA methylation levels were up‐regulated, corresponding to a delayed ripening process, while DNA methylation levels in the promoter of FaCYP707A4 were suppressed, correlating with increases in transcript and decreased ABA content. Experiments indicated FaCRY1, a blue light receptor repressed in bagged fruit and FaAGO4, a key protein involved in RNA‐directed DNA methylation, could bind to the promoter of FaCYP707A4. The interaction between FaCRY1 and FaAGO4, and an increased enrichment of FaAGO4 directed to the FaCYP707A4 promoter in fruit grown under light suggests FaCRY1 may influence FaAGO4 to modulate the DNA methylation status of the FaCYP707A4 promoter. Furthermore, transient overexpression of FaCRY1, or an increase in FaCRY1 transcription by blue light treatment, increases the methylation level of the FaCYP707A4 promoter, while transient RNA interference of FaCRY1 displayed opposite phenotypes. These findings reveal a mechanism by which DNA methylation influences ABA catabolism, and participates in light‐mediated strawberry ripening.
Hydrous shear zones are sites of melt transfer in the lower arc crust: A case study from Fiordland, New Zealand
Authors: Chatterjee Aditi , Daczko Nathan R. , Dey Joyjit , Piazolo Sandra 
Published: June 2024

AbstractRecent studies of the Cretaceous lower arc crust exposed in Fiordland, New Zealand, conclude that shear zones are sites of melt migration and mass transfer through the deep crust. Here, we investigate the 4–10 km‐wide George Sound Shear Zone, which cuts the Western Fiordland Orthogneiss, comprising two main rock types: two‐pyroxene gneiss and hornblende gneiss. Previous studies infer a predominantly igneous origin for the two types of gneiss. However, this study finds that melt‐rock interaction within the George Sound Shear Zone formed the hornblende gneiss from the precursor two‐pyroxene gneiss. Petrographic analyses of samples collected in transects across the shear zone show hydration reaction textures ranging from rims of hornblende + quartz around pyroxene grains to complete replacement of pyroxene grains. Plagioclase is recrystallized and partially replaced by clinozoisite. Additionally, biotite mode increases towards the higher strain rocks in the shear zone. Backscatter images and polarized light microscopy show microstructures indicative of former melt‐present deformation, including (a) interconnected mineral films of quartz and K‐feldspar along grain boundaries, (b) grains that terminate with low apparent dihedral angles, (c) interstitial grains, with some (d) undulose extinction in plagioclase and (e) serrated grain boundaries. In addition, zircon microstructures are consistent with Zr mobility, further supporting the former presence of melt; geochemical data show enrichment of Zr in the hornblende gneiss as compared to the two‐pyroxene gneiss. From the above observations, it is inferred that a felsic to intermediate hydrous melt migrated through the George Sound Shear Zone reacting with the host two‐pyroxene gneiss of the Western Fiordland Orthogneiss. Melt migration along grain boundaries was deformation assisted, (i) causing hydration of pyroxene to hornblende + quartz, and plagioclase to clinozoisite, (ii) increasing proportions of biotite within the shear zone and (iii) causing depletion of Cr + Ni and Zr enrichment in the hydrated rock. Our interpretation is supported by published observations of pegmatite dyke swarms that intruded into the George Sound Shear Zone, the P‐T conditions of deformation and characterization of microstructures that contrast sharply with those typically found in mylonitic rocks formed under solid‐state metamorphic conditions. Our results confirm that hydrous shear zones within otherwise anhydrous country rock are retrogressive and may represent evidence of melt migration through zones of deformation.
Compliance analysis of oral nutritional supplements in hospitalized postsurgical patients with colorectal cancer: A prospective study
Authors: Chen Yilin , Xian Xuemei , Zhu Chenping , Huang Binbin , Kuang Yuanyuan , Xu Didi 
Published: June 2024

AbstractPurposeThere are few reports on compliance with oral nutritional supplements (ONS) after surgery in hospitalized patients. This study aimed to investigate the compliance with ONS and its influencing factors after surgery in colorectal cancer patients.MethodsSTROBE checklist was used during the preparation of this paper. A total of 103 postoperative colorectal cancer patients were selected from November 2020 to April 2021 from the Department of Anorectal Surgery in our institution, which is a tertiary hospital in Hangzhou, China. The compliance with ONS was recorded by a self‐record sheet, and sociodemographic and disease‐related information of these patients was gathered through a self‐designed questionnaire.ResultsThe overall ONS compliance rate of patients with colorectal cancer was 57.6%. Results of univariate analysis revealed that ONS compliance rate was related to sex, admission body mass index, abdominal distension, attitude towards ONS, and satisfaction with ONS. Multi‐factor analysis showed that age, sex, and perceived benefits of nutrition therapy independently influenced the ONS compliance rate.ConclusionsThe postoperative ONS compliance rate of colorectal cancer patients needs to be further improved. Healthcare professionals should pay more attention to the postoperative ONS compliance and acknowledging the influence of age, gender, and attitudes towards ONS on ONS compliance. Notably, patients' perspectives towards ONS play a crucial and modifiable role in determining ONS compliance. Nurses ought to assist patients in cultivating a positive attitude towards ONS.Relevance to Clinical PracticeDosage form and delivery method are also influence factors that deserves further exploration in the future. Future research endeavours should endeavour to craft tailored, meticulous nutritional intervention strategies tailored to the diverse factors that influence ONS compliance, ultimately leading to enhanced ONS adherence. Our findings could serve as valuable evidence for the development of strategies aimed at enhancing ONS usage practices.
Does combining oro‐facial manual therapy with bruxism neuroscience education affect pain and function in cases of awake bruxism? A pilot study
Authors: von Piekartz Harry , Bleiss Sonja , Herzer Sebastian , Hall Toby , Ballenberger Nicolaus 
Published: June 2024

AbstractBackgroundAlthough awake bruxism is associated with temporomandibular disorder (TMD) as well as head and neck pain, the effects of physical therapy and bruxism education to address these factors have not been investigated.ObjectiveThe aim of this study was to evaluate the effects of oro‐facial manual therapy and bruxism neuroscience education (BNE) on awake bruxism over a 3‐week period with an open‐ended follow‐up questionnaire after 3 months.MethodsSubjects (n = 28) were randomly allocated to one of two groups, an intervention group and a control group. Data regarding disability, function and pain were collected pre‐ and post‐assessment, with all measures administered in a single‐blind fashion. Participants in both groups received six treatment sessions during this period. In addition to manual therapy, participants were provided with information on the neurophysiological mechanisms of bruxism and contributing factors. Individual behavioural guidelines and daily exercises were determined in consultation with the therapist. An introduction to a bruxism specific app (Brux.App) was also provided, which all participants used as an adjunct to their treatment.ResultsThe intervention group demonstrated notable improvement as indicated by their scores in the Neck Disability Index (NDI) (p = .008), Pain Disability Index (PDI) (p = .007) and Jaw Disability List (JDL) (p = .03). Furthermore, clinical assessments of the temporomandibular joint (TMJ) revealed a significant progress in terms of mouth opening (p = .03) and lateral jaw movement (laterotrusion) (p = .03). The mechanical pain threshold (PTT) of both the masseter (p = .02) and temporalis muscle (p = .05) also showed significant improvement. At 3‐month follow‐up, the questionnaire revealed that the majority of the intervention group (13/15, 87%) reported a benefit from the treatment.ConclusionThe reduction in pain and disability together with improvement in function and increased coping suggest a potential modification of awake bruxism through specialised musculoskeletal intervention and BNE tailored to the individual patient.
Interpretation in reverse: Remixing the Three Kingdoms
Authors: Wang Yiwen 
Published: June 2024

AbstractThis article investigates online fan video remixes that combine footage from the televisual adaptation of the Chinese classic The Romance of Three Kingdoms, set in ancient China, with soundtracks from the American science fiction film Inception (2010) and the magical fantasy Pirates of the Caribbean (2011). Analyzing the audio‐visual aesthetics of these videos, I propose a reversal of Henry Jenkins's “additive comprehension” framework, which suggests that fan works aim to interpret and complete the original story‐verse. Instead, I argue these videos reverse the process of interpretation through temporal–spatial transportation and corporeal transfiguration with false continuity editing, acousmatic aesthetics, and gravitational disorientation.