bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating methods for genome sequencing of Chlamydia trachomatis and other sexually transmitted bacteria directly from clinical swabs
Authors: Büttner Karina Andrea , Bregy Vera , Wegner Fanny , Purushothaman Srinithi , Imkamp Frank , Roloff Handschin Tim , Puolakkainen Mirja H. , Hiltunen-Back Eija , Braun Domnique , Kisakesen Ibrahim , Schreiber Andreas , Entrocassi Andrea Carolina , Gallo Vaulet María Lucía , López Aquino Deysi , Svidler López Laura , La Rosa Luciana , Egli Adrian , Rodríguez Fermepin Marcelo , Seth-Smith Helena MB ,   On behalf of the ESCMID Study Group for Mycoplasma and Chlamydia infections (ESGMAC)*
Published: February 2025

Graphical Abstract
Using target enrichment, RNA baits designed around a panel of bacterial sexually transmitted infections were used to fish target DNA from the clinical sample, leading to complete bacterial genome sequences.
Emergency Department Patients’ Perspectives on Being Offered HIV Pre-Exposure Prophylaxis (PrEP) Services in an Urban ED
Authors: Solnick Rachel E , Gonzalez-Argoti Tatiana , Bauman Laurie J. , Rael Christine Tagliaferri , Mantell Joanne E. , Calderon Yvonne , Cowan Ethan , Hoffman Susie 
Published: February 2025

ABSTRACTHIV pre-exposure prophylaxis (PrEP) is underutilized in the United States. Emergency Departments (EDs) can be strategic locations for initiating PrEP; however, knowledge concerning patients’ receptivity to ED PrEP programs is limited. This study explores ED patients’ perspectives on PrEP service delivery and their preferences for implementation. Semi-structured qualitative interviews were conducted with 15 potentially PrEP-eligible ED patients to examine their receptiveness to PrEP services, preferences for delivery methods, and logistical considerations. Most participants were open to learning about PrEP in the ED, provided it did not delay care, occur during distress, or compromise privacy. Universal PrEP education was viewed as reducing stigma and increasing awareness, while targeted screening was seen as efficient. Participants strongly preferred receiving information in person rather than via videos or pamphlets. Concerns included ensuring ED staff expertise and maintaining privacy during PrEP-related discussions. Regarding same-day PrEP versus prescriptions or referrals, opinions varied, with participants valuing flexibility and linkage to care. This first qualitative study of ED patients’ perspectives on PrEP services highlights general receptiveness, with key concerns about privacy, expertise, and wait times. Patient-centered approaches, including integrating PrEP services into ED workflows, offering flexible initiation options, and providing privacy, can support the feasibility of ED-based PrEP programs.
Seasonal changes in coat colour and sexual size dimorphism in a subtropical ungulate
Authors: Perroux Tania A. , McElligott Alan G. , Hodgson George M. W. , Flay Kate J. 
Published: February 2025

ABSTRACTPhenotypes reflect how organisms adapt to their environments. Hong Kong (HK) feral cattle, a crossbreed ofBos taurus taurusandBos taurus indicus, present an opportunity to study these adaptations in one of the very few global cattle populations not directly controlled by humans. These cattle are free-ranging since their release from farms in the 1970’s. HK has a subtropical climate, characterized by high humidity and temperatures during the wet season, and scarce precipitation during the dry season. We studied seasonal coat colour changes in HK feral cattle, and sexual dimorphism in body size and horn length. We provide the first evidence of seasonal changes in coat colour in cattle, with paler coats being more common in the wet season, while darker coats prevailed in the dry season. These seasonal changes were influenced by temperature, humidity, solar radiation, and body condition. We found that males were larger and had longer horns than females. Our results show a male-biased sex dimorphism in the HK feral cattle. Additionally, our findings suggest that thermoregulation costs drive colouration in these cattle. The phenotypic plasticity we demonstrate in these subtropical feral cattle improves our knowledge of the adaptations of ungulates to their habitat.
Learning From the Past: Medical School Experiences Predict Meaning in Work and Imposter Phenomenon Among Residents
Authors: Carpenter Kelsey M. , Falco Christopher M. , Menezes Sabrina , Diaz Jessica B. B. , Wells Jessica C. , Guldner Gregory , Siegel Jason T. 
Published: February 2025

AbstractIntroductionEndeavors to bolster resident well-being may benefit from a greater understanding of how associated psychological states are shaped across various stages of medical education. To this end, the current study aimed to determine if perceived psychological safety and exposure to negative aspects of the hidden curriculum during medical school predicted meaning in work and imposter phenomenon among medical residents transitioning into their new programs.MethodsAll incoming residents at a multi-institutional hospital organization were administered a survey link via email as part of a larger set of studies. Participants in this specific study (n= 237) completed measures assessing their perceptions of both positive and negative medical school experiences (i.e., psychological safety and hidden curriculum exposure) as well as their current feelings about themselves and their work (i.e., meaning in work and imposter phenomenon). Data collection took place during the summer of 2022.ResultsPerceptions of psychological safety during the last two years of medical school were positively related to meaning in work and negatively related to imposter phenomenon among incoming residents. Perceptions of exposure to negative aspects of the hidden curriculum predicted imposter phenomenon, but no link was observed between hidden curriculum exposure and meaning in work.DiscussionFeelings related to previous learning environments (i.e., medical school) may still be relevant during the transition into residency. Next steps include exploring potential causal linkages between these variables as well as expanding the scope and operationalization of psychological states and experiences considered across time points during the medical training journey.
Developing an anatomically valid segmentation protocol for anterior regions of the medial temporal lobe for neurodegenerative diseases
Authors: Sadeghpour Niyousha , Lim Sydney A. , Wuestefeld Anika , Denning Amanda E. , Ittyerah Ranjit , Trotman Winifred , Chung Eunice , Sadaghiani Shokufeh , Prabhakaran Karthik , Bedard Madigan L. , Ohm Daniel T. , Artacho-Pérula Emilio , Iñiguez de Onzoño Martin Maria Mercedes , Muñoz Monica , Molina Romero Francisco Javier , Delgado González José Carlos , Jiménez María del Mar Arroyo , Rabal Maria del Pilar Marcos , Insausti Serrano Ana María , González Noemí Vilaseca , Sánchez Sandra Cebada , de la Rosa Prieto Carlos , Insausti Ricardo , McMillan Corey , Lee Edward B. , Detre John A. , Das Sandhitsu R. , Xie Long , Tisdall M. Dylan , Irwin David J. , Wolk David A. , Yushkevich Paul A. , Wisse Laura EM. 
Published: February 2025

AbstractBackgroundThe anterior portion of the medial temporal lobe (MTL) is one of the first regions targeted by pathology in sporadic Alzheimer’s disease (AD) and Limbic-predominant Age-related TDP-43 Encephalopathy (LATE) indicating a potential for metrics from this region to serve as imaging biomarkers. Leveraging a unique post-mortem dataset of histology and magnetic resonance imaging (MRI) scans we aimed to 1) develop an anatomically valid segmentation protocol for anterior entorhinal cortex (ERC), Brodmann Area (BA) 35, and BA36 for in vivo 3 tesla (T) MRI and 2) incorporate this protocol in an automated approach.MethodsWe included 20 cases (61-97 years old, 50% females) with and without neurodegenerative diseases (11 vs. 9 cases) to ensure generalizability of the developed protocol. Digitized MTL Nissl-stained coronal histology sections from these cases were annotated and registered to same-subject post-mortem MRI. The protocol was developed by determining the location of histological borders of the MTL cortices in relation to anatomical landmarks. Subsequently the protocol was applied to 15 cases twice, with a 2-week interval, to assess intra-rater reliability with the Dice Similarity Index (DSI). Thereafter it was implemented in our in- house Automatic Segmentation of Hippocampal Subfields (ASHS)-T1 approach and evaluated with DSIs.ResultsThe anterior histological border distances of ERC, BA35 and BA36 were evaluated with respect to various anatomical landmarks and the distance relative to the beginning of the hippocampus was chosen. To formulate segmentation rules, we examined the histological sections for the location of borders in relationship to anatomical landmarks in the coronal sections. The DSI for the anterior MTL cortices for the intra-rater reliability was 0.85-0.88 and for the ASHS-T1 against the manual segmentation was 0.62-0.65.DiscussionWe developed a reliable segmentation protocol and incorporated it in an automated approach. Given the vulnerability of the anterior MTL cortices to tau deposition in AD and LATE, the updated approach is expected to improve imaging biomarkers for these diseases.
Variation in exposure in neighborhoods of Dhaka, Bangladesh across different environmental pathways: The influence of human behavior on fecal exposure in urban environments
Authors: Amin Nuhu , Raj Suraja , Green Jamie , Ali Shahjahan , Haque Sabrina , Wang Yuke , Mairinger Wolfgang , Ahmed Tanvir , Joseph George , Rahman Mahbubur , Moe Christine L. 
Published: February 2025

AbstractBackgroundPoor sanitation and fecal sludge management contribute to fecal contamination in Dhaka’s urban environment. An exposure assessment through nine-environmental pathways was conducted using the SaniPath tool to understand the risk of exposure to fecal contamination.MethodsData collection took place from April 2017-January 2018 in ten neighborhoods: four low-income, four high-income, and two transient/floating neighborhoods. A total of 900 environmental samples were analyzed using the IDEXX QuantiTray/2000 method with IDEXX-Colilert-24®media for the most probable number (MPN) ofE. coli. Additionally, 823 household surveys, 28 community surveys, and 35 school surveys on exposure-related behaviors were conducted. Bayesian methods were used to estimate monthlyE. coliexposure levels and population exposure percentages for each environmental pathway in the neighborhoods.ResultsFindings revealed that children (aged 5–12 years) in low-income and floating neighborhoods had more frequent contact with most environmental pathways (at least one-contact per-week or month), except surface water, compared to children in high-income neighborhoods. Dominant exposure pathways varied by neighborhood and socioeconomic status. Children experienced higher estimated monthly fecal exposure doses than adults, primarily through ingestion of contaminated municipal water (all community average=59.2%) and contact with open drains (52.3%) and surface waters (29.0%). Adults were mainly exposed via contaminated municipal water (54.2%), produce (87.0%), and street food (64.5%), which were frequently consumed.ConclusionsThese results highlight significant risks of fecal exposure across diverse urban settings in Dhaka. Integrated, multisectoral, and sustainable approaches are critical. Behavior change interventions targeting children and caregivers can further mitigate these risks and ensuring long-term public health improvements.
Association between Remnant Cholesterol and All-Cause and Cardiovascular Mortality Risk in Chronic Kidney Disease Patients Over 60: A Cohort Study Based on NHANES 2001-2018
Authors: YAO Jiechun , Lin Chun , Xu Qingbo , Li Guode , Lin Yan 
Published: February 2025

AbstractObjectiveChronic kidney disease (CKD) patients face significant mortality risks, and remnant cholesterol (RC) may be a critical prognostic indicator. This study aimed to explore the association between RC and all-cause and cardiovascular mortality risks in CKD patients.MethodsThis prospective cohort study utilized data from the National Health and Nutrition Examination Survey (NHANES) 2001-2018, including 1,606 CKD patients aged over 60. Cox proportional hazards models were employed to assess the association between RC and all-cause and cardiovascular mortality, with multi-model adjustments and subgroup analyses.ResultsDuring 93.9 months of follow-up, 932 all-cause mortality events (58.0%) and 283 cardiovascular mortality events (17.6%) were observed. Each logarithmic unit increase in RC was associated with a significant 41% reduction in all-cause mortality risk (adjusted HR: 0.59, 95% CI: 0.40-0.87, P=0.0079). Subgroup analyses revealed significantly reduced all-cause mortality risks in women (HR=0.71), hypertensive patients (HR=0.75), and type 2 diabetes patients (HR=0.66).ConclusionIn CKD patients, RC is significantly associated with reduced all-cause mortality risk, providing a new perspective for individualized mortality risk assessment.
Efficacy of optimized multichannel vs conventional tDCS for major depression treatment: A randomized controlled trial
Authors: Salehinejad Mohammad Ali , Abdi Marzieh , Dadashi Mohsen , Hallajian Amir-Homayun , Sharifi Kiomars , Khadem Ali , Salvador Ricardo , Ruffini Giulio , Nitsche Michael A. 
Published: February 2025

AbstractIntroductionTranscranial direct current stimulation (tDCS) has been proposed for treating depression. While it has advantages such as affordability, home-use application and fewer side effects its clinical efficacy is not yet established. This randomized, sham-controlled trial (NCT06165445) compares efficacy of an optimized multichannel protocol vs conventional and sham tDCS for depression treatment.Methods and MaterialsSixty patients with major depression received 30 sessions of conventional, optimized multichannel, or sham tDCS in parallel groups. In the multichannel optimized tDCS, electrical current was delivered via 7 small electrodes (1.65 mA max current per electrode, 4.0 mA total injected current per polarity) while in the conventional (2 mA max current per electrode, 2.0 mA total injected current per polarity) and sham tDCS large anodal and cathodal electrodes were used. Intervention efficacy and treatment response were evaluated before treatment and after the 10th, 20th, and 30th sessions, 1- and 3-months post-intervention. Cognitive functions and brain connectivity were also assessed.ResultsBoth active tDCS groups showed a significant reduction in depressive symptoms compared to the sham group, with the multichannel protocol demonstrating effects as early as the 10th session and achieving greater symptom alleviation than conventional tDCS. A response rate of 72.7% is observed, based on at least a 50% improvement in depressive scores from baseline at 1 month (Hedge’s g=2.80) and 3 months (Hedge’s g=2.31) post-treatment. Furthermore, the multichannel protocol improved cognitive functions and enhanced cerebral functional connectivity markers of depression pathophysiology.DiscussionThe optimized multichannel tDCS showed high efficacy for treating depression.
Dissociating the incubation of appetitive and consummatory behavior in a model of oral cocaine self-administration
Authors: Namba Mark D. , Goldberg Samuel L. , Side Christine M. , Yadlapalli Abhiram , Curran-Alfaro Christina M. , Kress Devin , Fogarty Melissa F. , Mohr Amanda L.A. , Barker Jacqueline M. 
Published: February 2025

AbstractCocaine use disorder remains a persistent public health dilemma that currently lacks effective treatment strategies. One key impediment to successful treatment outcomes is increased drug craving that occurs over the course of abstinence and subsequent relapse to drug use. This phenomenon, known as the incubation of drug craving, has been modeled extensively in rodent models of intravenous drug self-administration. As commonly implemented, the design of intravenous self-administration preclinical studies precludes disentangling appetitive and consummatory behaviors as drug seeking (appetitive) and taking (consummatory) is simultaneous. Here, we employed a model of oral cocaine self-administration to interrogate the incubation of drug vs nondrug craving, where the route of administration is identical between reinforcers and appetitive and consummatory behaviors are dissociable. Oral self-administration of cocaine produced detectable levels of cocaine and its metabolite, benzoylecgonine, within the blood and brain, and blood and brain levels of both substrates correlated with cocaine consumption. When tested for seeking-(lever pressing) and taking-related (magazine head entries) behavior after 1 or 21 days of forced abstinence from cocaine or saccharin, we observed incubation of lever pressing among cocaine-administering mice and incubation of magazine entries among saccharin-administering mice. These behavioral changes were accompanied by reduced expression of the glial glutamate transporter GLT-1 within the nucleus accumbens (NAc) of cocaine self-administering mice, regardless of abstinence. Altogether, these results underscore the utility of this model of cocaine self-administration, highlight the conserved nature of incubated cocaine seeking across routes of administration, and demonstrate the dissociable neurobehavioral sequelae of the incubation of reward seeking across reinforcer types.
Non-canonical internalization mechanisms of mGlu receptors
Authors: Cimadevila M , Liu J , Maurel D , Brabet I , Hoscar M , Drube J , Hoffmann C , Inoue A , Rondard P , Lafon PA , Prézeau L , Pin JP 
Published: February 2025

AbstractCell surface density of G protein-coupled receptors (GPCRs) is tightly regulated through constitutive and agonist-induced internalization. Whereas the mechanisms of constitutive internalization remain elusive, agonist-induced internalization is accepted to involve receptor phosphorylation by GPCR kinases (GRKs), β-arrestin binding and AP2 recruitment, targeting receptors to clathrin-coated pits. Dimeric class C metabotropic glutamate (mGlu1 to 8) receptors regulate synaptic transmission but their internalization process is ambiguous. Here, we used diffusion-enhanced energy transfer (DERET) to decipher their internalization kinetics. We showed that all mGlu receptors are constitutively internalized. However, only mGlu1, 5 and 3 homodimers are agonist-induced internalized, that require neither GRKs, nor β-arrestins. In contrast, the constitutive internalization involves only β-arrestins. This systematic study further illustrates how different class C receptors are relative to most other GPCRs, revealing non-canonical internalization mechanisms. These insights in mGlu receptor dynamics will help promoting the therapeutic action of drugs targeting mGlu receptors.
Indigenous peoples, environmental accountability and the semantic meaning of resource extraction firm disclosures
Authors: Liu Minqi , Taylor-Neu Kieran , Saxton Gregory D. , Neu Dean , Rahaman Abu S. , Everett Jeff 
Published: February 2025

PurposeThe study aims to explore how Indigenous peoples and their concerns become “entextualized” within the environmental disclosures of resource extraction firms.Design/methodology/approachA mixed-methods content analysis of 11,850 annual information forms filed by resource extraction firms with Canadian security regulators between 1997 and 2023 is conducted. FinBERT transformer encodings, agglomerative hierarchical clustering and computer-assisted techniques are combined with inductive analyses.FindingsThe findings show that, although Indigenous peoples and their concerns have become a more important element in environmental disclosures, dominant semantic meanings tend to view Indigenous people as impediments. At the same time, the entextualizations of Indigenous peoples and their concerns sometimes escape these dominant frames. Big firms appear to be no more likely to exhibit leadership or substantively take Indigenous peoples and their concerns into account than smaller firms.Originality/valueThe study offers a longitudinal perspective on how the environment and Indigenous peoples are portrayed in corporate disclosures. The study emphasizes the need to view environmental accountability as inextricably intertwined with accountability to Indigenous peoples and also illustrates the importance of identifying the semantic meanings that are being communicated. We propose that analyzing how and why specific semantic meanings about Indigenous peoples and their concerns become entextualized provides activists and policy-makers with a starting point for improved disclosure practices and, hopefully, better resource extraction practices.
Food safety knowledge among adult consumers in Bangladesh: a COVID-19 comparative study
Authors: Ishra Rakia , Sharif Saif , Soar Jeffrey , Khanam Rasheda 
Published: February 2025

PurposeSince the COVID-19 pandemic had a significant impact on all facets of civilisation around the world, including food safety, this study aimed to determine and compare the pre-COVID and post-COVID food safety knowledge of Bangladeshi consumers and their association with sociodemographic variables.Design/methodology/approachThe current study performed a cross-sectional survey of 503 consumers who prepared food at least 2–4 times per week through a validated questionnaire, selected through convenience sampling from two metropolitan cities and two rural districts in Bangladesh. The non-parametric Wilcoxon signed-rank test, Mann–Whitney U tests, Kruskal–Wallis H and binary logistic regression were used for statistical analysis.FindingsAlthough an increased percentage of consumers showed a better level of overall food safety knowledge in the post-pandemic (38.2%) than the pre-pandemic (28.8%), there is a low level of awareness of the appropriate duration of handwashing, foodborne pathogens, consuming raw milk or eggs, safe storage and temperature control of food. University graduates, families with few children and urban consumers had a good understanding of food safety both pre- and post-pandemic.Originality/valueThis study compares consumers’ pre- and post-COVID-19 food safety knowledge previously unknown in Bangladesh. The findings have significantly contributed to existing food safety knowledge in Bangladesh to adopt policies and structure training programmes for consumers in the country.
As lonely as the moon? When and how health beliefs of COVID-19 affect mental health, life satisfaction and performance
Authors: Ayala Yarid , Tordera Nuria , Karaeminogullari Aysegul , Bayona Jaime Andrés 
Published: February 2025

PurposeDrawing on the health belief model and affective events theory, the main objectives of this study are to: (1) analyze which health beliefs about COVID-19 (probability, seriousness and worry) trigger feelings of social isolation; (2) investigate whether psychological capital buffers the escalation of social isolation and (3) analyze the role of the feelings of social isolation as a mechanism that yields drawbacks on mental health, life satisfaction and performance.Design/methodology/approachData came from two waves of online surveys administered to 678 employees of a private university in Mexico.FindingsResults of structural equation modeling showed that beliefs of worry of getting COVID-19 trigger social isolation feelings, which, in turn, yields drawbacks on mental health, life satisfaction and performance (i.e. task, creative and organizational citizenship behavior). Moreover, psychological capital buffers the increases in feelings of social isolation generated by beliefs of the COVID-19 severity.Practical implicationsThis study provides insightful recommendations for handling future events that might imply social restrictions as a measure of contagion containment.Originality/valueWe contribute to Affective Events literature by linking it to the health belief model. A main criticism of affective events theory is its exogenous blindness and lack of attention to how affect reactions are triggered at work. We address this limitation by bridging health belief model and affective events theory to show what specific health beliefs of COVID-19 produce affects and behavioral reactions in employees.