bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rhabdomyosarcoma in adolescents
Authors: Bisogno Gianni , Compostella Alessia , Ferrari Andrea , Pastore Guido , Cecchetto Giovanni , Garaventa Alberto , Indolfi Paolo , De Sio Luigi , Carli Modesto 
Published: July 2011

AbstractBACKGROUND:In many types of cancer, the survival rates are reported to be less favorable for adolescents compared with younger children. To investigate whether this is true for adolescents with rhabdomyosarcoma (RMS), the results obtained in patients enrolled in protocols run by the Italian Soft Tissue Sarcoma Committee (STSC) were analyzed.METHODS:From 1988 through 2005, 643 patients were registered (567 children ages birth‐14 years and 76 adolescents ages 15‐19 years) and treated in 4 STSC protocols. The number of patients enrolled was compared with the expected number calculated from incidence rates derived from the Italian network of cancer registries.RESULTS:Only 27% of the expected number of adolescents with RMS were enrolled in the STSC trials. Compared with children, adolescents were found to have a longer interval from initial symptoms to diagnosis (8 weeks vs 4.6 weeks), more alveolar RMS (47.4% vs 32.6%), lymph node infiltration (39.1% vs 23.3%), and metastases at the time of diagnosis (30.7% vs 17.8%). The 2 age groups received similar treatments. The 5‐year overall survival (OS) rate was 68.9% in children versus 57.2% in adolescents (P = .006), and the progression‐free survival (PFS) rate was 64.3% in children versus 48.1% in adolescents (P = .0237). On multivariate analysis, age, tumor site, lymph node involvement, and metastases were found to be significant prognostic factors for OS and PFS.CONCLUSIONS:Survival for adolescents with RMS enrolled in STSC protocols appears to be satisfactory. The higher prevalence of unfavorable tumor characteristics noted among adolescents seems to explain their worse outcome compared with children. However, the limited number of adolescents enrolled in STSC studies is worrisome, and cooperation with oncologists who treat adults needs to be improved. Cancer 2012;. © 2011 American Cancer Society.
Role of BRAFV600E mutation analysis and second cytologic review of fine‐needle aspiration for evaluating thyroid nodule
Authors: Kang Guhyun , Cho Eun Yoon , Shin Jung Hee , Chung Jae‐Hoon , Kim Jong Won , Oh Young Lyun 
Published: July 2011

AbstractBACKGROUND:Thyroid fine‐needle aspiration (FNA) is the primary diagnostic tool used for the evaluation of thyroid nodules. Although most aspirates provide diagnostic cytology, some are classified as indeterminate. The aim of this study was to determine whether the second review of FNA cytology can improve the diagnostic values and to assess the role of proto‐oncogene B‐Raf (BRAF) mutation testing in the diagnosis of papillary carcinoma (PC).METHODS:Thyroid aspirates from 1060 patients were submitted for cytologic evaluation and BRAFV600E mutation analysis. A second cytologic review was performed by 2 cytopathologists in light of the mutation status.RESULTS:Of the 313 patients who received surgery, 200 (63.9%) had been initially diagnosed as malignant by cytology. They were surgically confirmed as PCs, and the BRAFV600E mutation was detected in 82.5% of the cases. Ninety‐five of 102 cases (93.1%) with indeterminate cytology turned out to be malignant, and the mutation was present in 63.3% of PCs. The sensitivity, accuracy, and negative predictive value (NPV) of the second review were better than those of initial cytologic diagnosis (P <.001). The addition of the mutation analysis significantly increased the sensitivity, accuracy, and NPV for detecting PCs compared with those of cytology alone.CONCLUSIONS:Qualified cytologic diagnosis increases the effectiveness of FNA, forgoing the need for repeat biopsy or intraoperative frozen section evaluation. Preoperative BRAF mutation testing can supplement the routine cytology in the selection of thyroid nodules for surgery. Cancer (Cancer Cytopathol) 2012. © 2011 American Cancer Society.
Surface‐Plasmon‐Coupled Emission of Rhodamine 110 in a Silica Nanolayer
Authors: Rangełowa‐Jankowska Simeonika , Jankowski Dawid , Grobelna Beata , Gryczyński Ignacy , Gryczyński Zygmunt , Bogdanowicz Robert , Bojarski Piotr 
Published: July 2011

AbstractThe first observation of strong directional surface‐plasmon‐coupled emission (SPCE) of Rhodamine 110 in silica nanofilms deposited on silver nanolayers is reported. The preparation of the material is described in detail. The intensity of SPCE exceeds 10 times that of free space fluorescence and total linear light polarization in the SPCE ring is observed. A new experimental setup and an original data collection method is presented. Our material completely preserves its fluorescence properties for at least eight months.
Thymic but not splenic CD8+ DCs can efficiently cross‐prime T cells in the absence of licensing factors
Authors: Dresch Christiane , Ackermann Mathias , Vogt Bernd , de Andrade Pereira Bruna , Shortman Ken , Fraefel Cornel 
Published: July 2011

AbstractCross‐presentation is an important mechanism to elicit both immune defenses and tolerance. Although only a few DC subsets possess the machinery required for cross‐presentation, little is known about differences in cross‐presenting capabilities of DCs belonging to the same subpopulation but localized in different lymphoid organs. In this study, we demonstrate that steady‐state thymic CD8+ DCs can efficiently cross‐prime naïve CD8+ T cells in the absence of costimulation. Surprisingly, cross‐priming by splenic CD8+ DCs was dependent on licensing factors such as GM‐CSF. In the absence of GM‐CSF, antigen–MHC‐class‐I complexes were detected on thymic but not on splenic CD8+ DCs, indicating that the cross‐presentation capacity of the thymic subpopulation was higher. The observed cross‐priming differences between thymic and splenic CD8+ DCs did not correlate with differential antigen capture or costimulatory molecules found on the surface of DCs. Moreover, we did not detect overall impairment of antigen presentation, as peptide‐loaded splenic CD8+ DCs were able to induce CD8+ T‐cell proliferation. The observation that thymic CD8+ DCs are more efficient than splenic CD8+ DCs in T‐cell cross‐priming in the absence of licensing factors indicates that the requirements for efficient antigen presentation differ between these cells.
Bis(2‐methylbenzimidazolyl)amine‐Derived Copper Complexes and Their Antineoplastic Activity
Authors: Rodríguez Solano Laura A. , Aguiñiga Itzen , López Ortiz Manuel , Tiburcio Reynaldo , Luviano Axel , Regla Ignacio , Santiago‐Osorio Edelmiro , Ugalde‐Saldívar Víctor M. , Toscano Rubén A. , Castillo Ivan 
Published: July 2011

AbstractCopper(II) complexes (1–4) of tri‐ or tetradentate bis(2‐methylbenzimidazolyl)amine ligands (L1–L4) have been prepared and characterized by spectroscopic methods in solution, as well as in the solid state by X‐ray crystallography. The ligands act as tridentate donors towards the cupric ions through one central amine and two benzimidazole N atoms in the solid state; a water ligand and a bridging perchlorate group define the distorted octahedral environments of2and3. Complex4has square‐pyramidal coordination geometry, with an additional thioether donor attached to the central N atom in the axial position. Electrospray mass spectrometry characterized the complexes as monomeric in acetonitrile solution. Cyclic voltammetry studies established that amine N benzyl‐containing3has the highest half‐wave redox potential of all of these complexes at –0.08 V (vs. Fc+/Fc) for the Cu2+/Cu+couple. The complexes display dose‐dependent cytotoxicity against one human and four murine cancer cell lines. Complexes3and4have good antiproliferative activity against the human chronic leukemia cell line K562. Moreover, for3the proliferation of all malignant cell lines decreases at concentrations lower than the IC50for healthy bone marrow cells.
Simultaneous Determination of Pb and Cd Traces in Water Samples by Anodic Stripping Voltammetry Using a Modified GC Electrode
Authors: Stankovic Dalibor , Manojlovic Dragan , Roglic Goran , Kostic‐Rajacic Sladjana , Andjelkovic Ivan , Dojcinovic Biljana , Mutic Jelena 
Published: July 2011

AbstractThe determination of Pb and Cd with a Nafion‐modified glassy carbon electrode and Cu‐DPABA complex (Cu‐DPABA–NA/GCE; DPABA is methyl 3,5‐bis{bis‐[(pyridin‐2‐yl)methyl]amino}methyl‐benzoate) as an alternative electrode for anodic stripping voltammetry was described. Pb and Cd were accumulated in acetate buffer pH 4 at a potential of −1.4 V (vs. Ag/AgCl electrode) for 120 s followed by a DPASV scan from −1.2 to −0.2 V. Under optimum conditions the calibration curves were linear in the range of 4.8×10−9–5.0×10−5 and 5.0×10−9–5×10−5 mol L−1 for Pb and Cd, respectively. Detection limits were 1.8×10−9 and 1.2×10−9 mol L−1 for Pb and Cd, respectively. Different parameters and conditions, such as membrane ingredients, accumulation time, potential and pH value were optimized. A study of interfering substances was also performed. A significant increase in current was achieved at the modified electrode in comparison with the bare glassy carbon electrode. The validation of the proposed method was made by Pb and Cd determination in the certified reference material Groundwater CRM 610 (BCR, Community Bureau of Reference, Brussels, Belgium). The electrode was successfully applied for determination of Pb and Cd in river water with a high content of organic contaminants without any pretreatment.
Numerical prediction of turbulent flow structure generated by a synthetic cross‐jet into a turbulent boundary layer
Authors: Javadi Ardalan , El‐Askary Wageeh A. 
Published: July 2011

SUMMARYA detailed numerical study using large‐eddy simulation (LES) and unsteady Reynolds‐averaged Navier–Stokes (URANS) was undertaken to investigate physical processes that are engendered in the injection of a circular synthetic (zero‐net mass flux) jet in a zero pressure gradient turbulent boundary layer. A complementary study was carried out and was verified by comparisons with the available experimental data that were obtained at corresponding conditions with the aim of achieving an improved understanding of fluid dynamics of the studied processes. The computations were conducted by OpenFOAM C++, and the physical realism of the incoming turbulent boundary layer was secured by employing random field generation algorithm. The cavity was computed with a sinusoidal transpiration boundary condition on its floor. The results from URANS computation and LES were compared and described qualitatively and quantitatively. There is a particular interest for acquiring the turbulent structures from the present numerical data. The numerical methods can capture vortical structures including a hairpin (primary) vortex and secondary structures. However, the present computations confirmed that URANS and LES are capable of predicting current flow field with a more detailed structure presented by LES data as expected. Copyright © 2011 John Wiley & Sons, Ltd.
Coordination polymers based on building blocks of dimethyltin(IV) Chloride iso‐thiocyanate and dimethyltin(IV) di‐iso‐thiocyanate with pyrazine‐2‐carboxylate and 4, 4′‐bipyridine
Authors: Najafi Ezzatollah , Amini Mostafa M. , Sadeghi Omid , Khavasi Hamid Reza , Ng Seik Weng 
Published: July 2011

AbstractThe reaction of 4,4′‐bipy with dimethyltin(IV) chloride iso‐thiocyanate affords the one‐dimensional (1D) coordination polymer, [Me2Sn(NCS)Cl·(4,4′‐bipy)]n (1), whereas reaction of dimethyltin(IV) dichloride with sodium pyrazine‐2‐carboxylate in the presence of potassium iso‐thiocyanate affords the two‐dimensional (2D) coordination polymer, {[Me2Sn(C4H3N2COO)2]2 [Me2Sn(NCS)2]}n (2). Both coordination polymers were characterized by elemental analysis and infrared spectroscopy in addition to 1H and 13C NMR spectroscopy of the soluble coordination polymer (1). A single‐crystal structure determination showed that the asymmetric unit in 1 contains Me2Sn(NCS)Cl and 4,4′‐bipy moieties and a 1D infinite rigid chain structure forms through bridging of the 4,4′‐bipy ligand between tin atoms and the geometry around the tin atom is a distorted octahedral. Coordination polymer 2 contains two distinct tin atom geometrics in which one tin atom is seven coordinate, and the other is six coordinate. The two tin atom environments are best described as a pentagonal bipyramidal in the former and distorted octahedral in the latter where the carboxylate groups bridge the two tin atoms and construct a 2D‐coordination polymer. The 119Sn NMR spectroscopy indicates the octahedral geometry of 1 retains in solution. © 2011 Wiley Periodicals, Inc. Heteroatom Chem 22:699–706, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/.20736
Synthesis and use of borane and platinum(II) complexes of 3‐diphenylphosphino‐1‐ phenylphospholane (LuPhos)
Authors: Pietrusiewicz K. Michał , Flis Anna , Ujj Viktória , Körtvélyesi Tamás , Drahos László , Pongrácz Péter , Kollár László , Keglevich György 
Published: July 2011

Abstract3‐Diphenylphosphinoyl‐1‐phenylphospholane 1‐oxide (2) obtained by the Michael addition of diphenylphosphine oxide to the double‐bond of 1‐phenyl‐2‐phospholene 1‐oxide (1) was subjected to double deoxygenation to afford the corresponding bisphosphine (3, LuPhos) that was converted to bis(phosphine borane) 4 and to cis chelate platinum(II) complex 6. A mixed phosphine oxide–phosphine borane 5 was also prepared. Stereostructures of the bidentate P‐ligand 3 and the ring platinum(II) complex (6) were evaluated by quantum chemical calculations. Complex 6 used as a catalyst showed modest activity, but unusual regioselectivity in the hydroformylation of styrene and its 4‐substituted analogues. © 2011 Wiley Periodicals, Inc. Heteroatom Chem 22:730–736, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/hc.20741
The synthesis and tuberculostatic activity of benzenesulfonohydrazide derivatives
Authors: Wisterowicz Krystyna , Gobis Katarzyna , Foks Henryk , Augustynowicz‐Kopeć Ewa 
Published: July 2011

AbstractThe series of novel N′‐aminocarbonothioyl‐benzenesulfonohydrazides were synthesized in a reaction of benzenesulphonyl chlorides with various aminocarbothiohydrazides. The structures were confirmed by IR and NMR spectra as well as elemental analysis. All of the obtained compounds were screened in vitro for their tuberculostatic activity. Preliminary results indicated that some target compounds exhibited promising results, especially toward Mycobacterium tuberculosis (Mtb) resistant strain 210. © 2011 Wiley Periodicals, Inc. Heteroatom Chem 23:99–104, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/hc.20743
HDAC inhibition promotes cardiogenesis and the survival of embryonic stem cells through proteasome‐dependent pathway
Authors: Chen Hong P. , DeNicola Megan , Qin Xin , Zhao Yu , Zhang Ling , Long Xi L. , Zhuang Shougang , Liu Paul Y. , Zhao Ting C. 
Published: July 2011

AbstractHistone deacetylase (HDAC) inhibition plays a crucial role in mediating cardiogenesis and myocardial protection, whereas HDAC degradation has recently attracted attention in mediating the biological function of HDACs. However, it remains unknown whether HDAC inhibition modulates cardiogenesis and embryonic stem cell (ESC) survival through the proteasome pathway. Using the well‐established mouse ESC culture, we demonstrated that HDAC inhibitors, both trichostatin A (TSA,50 nmol/L) and sodium butyrate (NaB, 200 µmol/L) that causes the pronounced reduction of HDAC4 activity, decreased cell death and increased viability of ESCs in response to oxidant stress. HDAC inhibition reduced the cleaved caspases 3, 6, 9, PARP, and TUNEL positive ESCs, which were abrogated with MG132 (0.5 µmol/L), a specific proteasome inhibitor. Furthermore, HDAC inhibition stimulates the growth of embryoid bodies (EB), which are associated with a faster spontaneous rhythmic contraction. HDAC inhibition increases the up‐regulation of GATA4, MEF2C, Nkx2.5, cardiac actin, and α‐SMA mRNA and protein levels that were abrogated by MG132. TSA and NaB resulted in a significant increase in cardiac lineage commitments that were blocked by the proteasome inhibition. Notably, HDAC inhibitors led to noticeable HDAC4 degradation, which was effectively prevented by MG132. Luciferase assay demonstrates an activation of MEF2 cardiac transcriptional factor by HDAC inhibition, which was repressed by MG132, revealing that the degradation of HDAC4 allows for the activation of MEF2. Taken together, our study is the first to demonstrate that HDAC inhibition through proteasome pathway forms a novel signaling to determine the cardiac lineage commitment and elicits the survival pathway. J. Cell. Biochem. 112: 3246–3255, 2011. © 2011 Wiley Periodicals, Inc.
Transgenic expression of an altered angiotensin type I AT1 receptor resulting in marked modulation of vascular type I collagen
Authors: Yu Jun , Taylor Linda , Rich Celeste , Toselli Paul , Stone Philip , Green Daniel , Warburton Rod , Hill Nicholas , Goldstein Ronald , Polgar Peter 
Published: July 2011

AbstractThe angiotensin II (AngII) type I receptor (AT1) was modified by replacing its third intracellular loop and C‐terminal tail with the corresponding regions from the bradykinin B2 receptor. Transgenic mice were produced that overexpress this mutated receptor (AB3T). Considerably less collagen content in the intact aorta and in primary aortic smooth muscle cells (aSMCs) cultures was observed in the transgenic mice. On the other hand, elastin content remained unchanged as measured by Western blot, and insoluble amino acid quantitation. The contraction of isolated aortas also remained unaltered. The aSMCs derived from the transgenic mice showed a reduction in AngII responsive type I collagen production. In aSMCs from transgenic mice, the cascade of Akt to the mammalian target rapamycin (mTOR) to p70 S6 kinase (p70S6K) was not AngII activated, while in the aSMCs from wild‐type (WT) mice the cascade was AngII activated. Angiotensin activation of Smad2 and Stat3 was also reduced in the AB3T aSMCs. However, no change in the effect of transforming growth factor β (TGFβ) on type I collagen production was observed. Also, the activation of ERK and JNK and G‐protein linked signaling remained unaltered in response to AngII. Akt and PI3K activation inhibitors blocked AngII‐stimulated type I collagen expression in WT aSMCs, whereas ERK inhibitor had no such effect. Our results point to an Akt/mTOR/p70S6K regulation of collagen production by AngII with participation of Smad2 and Stat3 cascades in this process. J. Cell. Physiol. 227: 2013–2021, 2012. © 2011 Wiley Periodicals, Inc.
Green microalga Chlorella vulgaris as a potential feedstock for biodiesel
Authors: Mallick Nirupama , Mandal Shovon , Singh Amit Kumar , Bishai Moumita , Dash Archana 
Published: July 2011

AbstractBACKGROUND: A major bottleneck in microalgal biodiesel production is lipid content, which is often low in microalgal species. The present study examines Chlorella vulgaris as a potential feedstock for biodiesel by identifying and evaluating the relationships between the critical variables that enhance the lipid yield, and characterizes the biodiesel produced for various properties.RESULTS: Factors affecting lipid accumulation in a green microalga, Chlorella vulgaris were examined. Multifactor optimization raised the lipid pool to 55% dry cell weight against 9% control. When C. vulgaris cells pre‐grown in glucose (0.7%)‐supplemented medium were transferred to the optimized condition at the second stage, the lipid yield was boosted to 1974 mg L−1, a value almost 20‐fold higher than for the control. The transesterified C. vulgaris oil showed the presence of ∼82% saturated fatty acids, with palmitate and stearate as major components, thus highlighting the oxidative stability of C. vulgaris biodiesel. The fuel properties (density, viscosity, acid value, iodine value, calorific value, cetane index, ash and water contents) are comparable with the international (ASTM and EN) and Indian (IS) biodiesel standards.CONCLUSION: C. vulgaris biomass with 55% lipid content and adequate fuel properties is potentially a renewable feedstock for biodiesel. Copyright © 2011 Society of Chemical Industry
Comparison of diffusion‐weighted MRI and MR volumetry in the evaluation of early treatment outcomes after preoperative chemoradiotherapy for locally advanced rectal cancer
Authors: Kim Young Chul , Lim Joon Seok , Keum Ki Chang , Kim Kyung Ah , Myoung Sungmin , Shin Sang Joon , Kim Myeong‐Jin , Kim Nam Kyu , Suh Jinsuk , Kim Ki Whang 
Published: July 2011

AbstractPurpose:To compare diffusion‐weighted imaging (DWI) and magnetic resonance (MR) volumetry for predicting treatment outcomes of locally advanced rectal cancers with preoperative chemoradiotherapy (CRT).Materials and Methods:This prospective study was approved by our Institutional Review Board. Thirty‐four patients underwent three MR examinations: pre‐CRT (before CRT), early CRT (2 weeks after CRT initiation), and post‐CRT (before surgery). The tumor apparent diffusion coefficient (ADC), ADC increase rate, and volume reduction rate were compared between responders and nonresponders using three reference standards: downstaging, modified Response Evaluation Criteria in Solid Tumors (mRECIST), and tumor regression grade (TRG). For DWI and volumetry, differences between responders and nonresponders were assessed by receiver operating characteristic analysis.Results:The median early tumor volume reduction rate of responders, subgrouped by downstaging and mRECIST (47.97% and 53.97%, respectively), was significantly higher than that of nonresponders (20.94% and 20.36%; P = 0.0024 and 0.0001, respectively), but there were no significant differences in pre‐CRT ADC and early ADC increase rate using all references. When using the downstaging and mRECIST, the diagnostic performance of early tumor volume reduction rate (Az = 0.81 and 0.94, respectively) was higher than that of pre‐CRT ADC (Az = 0.55 and 0.62; P = 0.033 and 0.007) and early ADC increase rate (Az = 0.58 and 0.64; P = 0.055 and 0.01) for predicting the treatment outcome. For TRG, there were no significant differences between DWI and volumetry.Conclusion:Early tumor volume reduction rate at the second week after CRT initiation may be a better indicator than DWI based on the mean ADC measurements for predicting CRT treatment outcome. J. Magn. Reson. Imaging 2011;. © 2011 Wiley‐Liss, Inc.