bluuebunny/crossref_metadata_2025_split huggingface dataset:
30 years of youth system of care lessons learned – a qualitative study of Hawaiʻi’s partnership with the Substance Abuse and Mental Health Services Administration
Authors: Okamura Kelsie H. , Jackson David , Ching Danielle L. Carreira , Suh Da Eun , Hartsock Tia L. R. , Hee Puanani J. , Shimabukuro Scott K. 
Published: May 2024

Abstract
Background
The Hawaiʻi State Department of Health, Child and Adolescent Mental Health Division (CAMHD) has maintained a longstanding partnership with Substance Abuse and Mental Health Services Administration (SAMHSA) to enhance capacity and quality of community-based mental health services. The current study explored CAMHD’s history of SAMHSA system of care (SOC) awards and identified common themes, lessons learned, and recommendations for future funding.

Methods
Employing a two-phase qualitative approach, the study first conducted content analysis on seven final project reports, identifying themes and lessons learned based on SOC values and principles. Subsequently, interviews were conducted with 11 system leaders in grant projects and SOC award projects within the state. All data from project reports and interview transcripts were independently coded and analyzed using rapid qualitative analysis techniques.

Results
Content validation and interview coding unveiled two content themes, interagency collaboration and youth and family voice, as areas that required long-term and consistent efforts across multiple projects. In addition, two general process themes, connection and continuity, emerged as essential approaches to system improvement work. The first emphasizes the importance of fostering connections in family, community, and culture, as well as within workforce members and child-serving agencies. The second highlights the importance of nurturing continuity throughout the system, from interagency collaboration to individual treatment.

Conclusions
The study provides deeper understanding of system of care evaluations, offering guidance to enhance and innovate youth mental health systems. The findings suggest that aligning state policies with federal guidelines and implementing longer funding mechanisms may alleviate administrative burdens.
Enhancing surgical outcomes in elderly gastric cancer patients: the role of comprehensive preoperative assessment and support
Authors: Ushimaru Yuki , Nagano Shinnosuke , Kawabata Ryohei , Nishikawa Kazuhiro , Takeoka Tomohira , Kitagawa Akihiro , Ohara Nobuyoshi , Tomihara Hideo , Maeda Sakae , Imasato Mitsunobu , Noura Shingo , Miyamoto Atsushi 
Published: May 2024

Abstract
Background
As the prevalence of gastric cancer rises in aging populations, managing surgical risks and comorbidities in elderly patients presents a unique challenge. The Comprehensive Preoperative Assessment and Support (CPAS) program, through comprehensive preoperative assessments, aims to mitigate surgical stress and improve outcomes by enhancing patient awareness and preparation. This study investigates the efficacy of a CPAS program, incorporating frailty and sarcopenia evaluations, to improve short-term outcomes in elderly gastric cancer patients.

Methods
A retrospective analysis was conducted on 127 patients aged 75 or older who underwent surgery with CPAS between 2018 and August 2023, compared to 170 historical controls from 2012 to 2017. Propensity score matching balanced both groups based on age-adjusted Charlson Comorbidity Index and surgical details. The primary focus was on the impact of CPAS elements such as rehabilitation, nutrition, psychological support, oral frailty, and social support on short-term surgical outcomes.

Results
Among 83 matched pairs, the CPAS group, despite 40.4% of patients in the CPAS group and 21.2% in the control group had an ASA-PS score of 3 or higher (P < 0.001), demonstrated significantly reduced blood loss (100 ml vs. 190 ml, P = 0.026) and lower incidence of serious complications (19.3% vs. 33.7%, P = 0.034), especially in infections and respiratory issues. Sarcopenia was identified in 38.6% of CPAS patients who received tailored support. Additionally, the median postoperative hospital stay was notably shorter in the CPAS group (10 days vs. 15 days, P < 0.001), with no in-hospital deaths. These results suggest that personalized preoperative care effectively mitigates operative stress and postoperative complications.

Conclusion
Implementing CPAS significantly enhances surgical safety and reduces complication rates in elderly gastric cancer patients, emphasizing the critical role of personalized preoperative care in surgical oncology for this demographic.
FruitPhenoBox – a device for rapid and automated fruit phenotyping of small sample sizes
Authors: Kirchgessner Norbert , Hodel Marius , Studer Bruno , Patocchi Andrea , Broggini Giovanni A. L. 
Published: May 2024

Abstract
Background
Fruit appearance of apple (Malus domestica Borkh.) is accession-specific and one of the main criteria for consumer choice. Consequently, fruit appearance is an important selection criterion in the breeding of new cultivars. It is also used for the description of older varieties or landraces. In commercial apple production, sorting devices are used to classify large numbers of fruit from a few cultivars. In contrast, the description of fruit from germplasm collections or breeding programs is based on only a few fruit from many accessions and is mostly performed visually by pomology experts. Such visual ratings are laborious, often difficult to compare and remain subjective.

Results
Here we report on a morphometric device, the FruitPhenoBox, for automated fruit weighing and appearance description using computer-based analysis of five images per fruit. Recording of approximately 100 fruit from each of 15 apple cultivars using the FruitPhenoBox was rapid, with an average handling and recording time of less than eleven seconds per fruit. Comparison of fruit images from the 15 apple cultivars identified significant differences in shape index, fruit width, height and weight. Fruit shape was characteristic for each cultivar, while fruit color showed larger variation within sample sets. Assessing a subset of 20 randomly selected fruit per cultivar, fruit height, width and weight were described with a relative margin of error of 2.6%, 2.2%, and 6.2%, respectively, calculated from the mean value of all available fruit.

Conclusions
The FruitPhenoBox allows for the rapid and consistent description of fruit appearance from individual apple accessions. By relating the relative margin of error for fruit width, height and weight description with different sample sizes, it was possible to determine an appropriate fruit sample size to efficiently and accurately describe the recorded traits. Therefore, the FruitPhenoBox is a useful tool for breeding and the description of apple germplasm collections.
Region-specific activation in the accumbens nucleus by itch with modified scratch efficacy in mice – a model-free multivariate analysis
Authors: Inokuchi-Sakata Sanae , Narita Ryo , Takahashi Yukari , Ishiuji Yozo , Asahina Akihiko , Kato Fusao 
Published: May 2024

AbstractItch is a protective/defensive function with divalent motivational drives. Itch itself elicits an unpleasant experience, which triggers the urge to scratch, relieving the itchiness. Still, it can also result in dissatisfaction when the scratch is too intense and painful or unsatisfactory due to insufficient scratch effect. Therefore, it is likely that the balance between the unpleasantness/pleasure and satisfaction/unsatisfaction associated with itch sensation and scratching behavior is determined by complex brain mechanisms. The physiological/pathological mechanisms underlying this balance remain largely elusive. To address this issue, we targeted the "reward center" of the brain, the nucleus accumbens (NAc), in which itch-responsive neurons have been found in rodents. We examined how neurons in the NAc are activated or suppressed during histamine-induced scratching behaviors in mice. The mice received an intradermal injection of histamine or saline at the neck, and the scratching number was analyzed by recording the movement of the bilateral hind limbs for about 45 min after injection. To experimentally manipulate the scratch efficacy in these histamine models, we compared histamine's behavioral and neuronal effects between mice with intact and clipped nails on the hind paws. As expected, the clipping of the hind limb nail increased the number of scratches after the histamine injection. In the brains of mice exhibiting scratching behaviors, we analyzed the expression of the c-fos gene (Fos) as a readout of an immediate activation of neurons during itch/scratch and dopamine receptors (Drd1 and Drd2) using multiplex single-molecule fluorescence in situ hybridization (RNAscope) in the NAc and surrounding structures. We performed a model-free analysis of gene expression in geometrically divided NAc subregions without assuming the conventional core–shell divisions. The results indicated that even within the NAc, multiple subregions responded differentially to various itch/scratch conditions. We also found different clusters with neurons showing similar or opposite changes in Fos expression and the correlation between scratch number and Fos expression in different itch/scratch conditions. These regional differences and clusters would provide a basis for the complex role of the NAc and surrounding structures in encoding the outcomes of scratching behavior and itchy sensations.
Critical reappraisal of short-acting bronchodilators for pediatric respiratory diseases
Authors: Licari Amelia , Manti Sara , Mastellone Francesco , Miraglia Del Giudice Michele , Marseglia Gian Luigi , Tosca Maria Angela ,   Asthma and Personalized Therapies Committee of the Italian Society of Pediatric Allergy & Immunology (SIAIP), Andrenacci Beatrice , Capristo Carlo , Cutrera Renato , Di Cicco Maria Elisa , Fierro Vincenzo , Leone Maddalena , Naso Matteo , Pezone Ilaria , Trincianti Chiara 
Published: May 2024

AbstractShort-acting bronchodilators are a class of medications commonly used to treat asthma, chronic obstructive pulmonary disease, and other respiratory conditions. The use of these medications has evolved over time as we have gained a better understanding of their effectiveness and safety in the pediatric population. This comprehensive review synthesizes the current understanding of short-acting β2-agonists and short-acting anticholinergics in children. It addresses indications, contraindications, safety considerations, and highlights areas where further research is needed to guide the most effective use of short-acting bronchodilators.
Dyspnea is severe and associated with a higher intubation rate in de novo acute hypoxemic respiratory failure
Authors: Demoule Alexandre , Baptiste Amandine , Thille Arnaud W. , Similowski Thomas , Ragot Stephanie , Prat Gwénael , Mercat Alain , Girault Christophe , Carteaux Guillaume , Boulain Thierry , Perbet Sébastien , Decavèle Maxens , Belin Lisa , Frat Jean-Pierre ,   from the REVA Network (Research Network in Mechanical Ventilation)
Published: May 2024

Abstract

Background
Dyspnea is a key symptom of de novo acute hypoxemic respiratory failure. This study explores dyspnea and its association with intubation and mortality in this population.

Methods
This was a secondary analysis of a multicenter, randomized, controlled trial. Dyspnea was quantified by a visual analog scale (dyspnea-VAS) from zero to 100 mm. Dyspnea was measured in 259 of the 310 patients included. Factors associated with intubation were assessed with a competing risks model taking into account ICU discharge. The Cox model was used to evaluate factors associated with 90-day mortality.

Results
At baseline (randomization in the parent trial), median dyspnea-VAS was 46 (interquartile range, 16–65) mm and was ≥ 40 mm in 146 patients (56%). The intubation rate was 45%. Baseline variables independently associated with intubation were moderate (dyspnea-VAS 40–64 mm) and severe (dyspnea-VAS ≥ 65 mm) dyspnea at baseline (sHR 1.96 and 2.61, p = 0.023), systolic arterial pressure (sHR 2.56, p < 0.001), heart rate (sHR 1.94, p = 0.02) and PaO2/FiO2 (sHR 0.34, p = 0.028). 90-day mortality was 20%. The cumulative probability of survival was lower in patients with baseline dyspnea-VAS ≥ 40 mm (logrank test, p = 0.049). Variables independently associated with mortality were SAPS 2 ≥ 25 (p < 0.001), moderate-to-severe dyspnea at baseline (p = 0.073), PaO2/FiO2 (p = 0.118), and treatment arm (p = 0.046).

Conclusions
In patients admitted to the ICU for de novo acute hypoxemic respiratory failure, dyspnea is associated with a higher risk of intubation and with a higher mortality.

Trial registration: clinicaltrials.gov Identifier # NCT 01320384.
Identification of the RSX interactome in a marsupial shows functional coherence with the Xist interactome during X inactivation
Authors: McIntyre Kim L. , Waters Shafagh A. , Zhong Ling , Hart-Smith Gene , Raftery Mark , Chew Zahra A. , Patel Hardip R. , Graves Jennifer A. Marshall , Waters Paul D. 
Published: May 2024

AbstractThe marsupial specific RSX lncRNA is the functional analogue of the eutherian specific XIST, which coordinates X chromosome inactivation. We characterized the RSX interactome in a marsupial representative (the opossum Monodelphis domestica), identifying 135 proteins, of which 54 had orthologues in the XIST interactome. Both interactomes were enriched for biological pathways related to RNA processing, regulation of translation, and epigenetic transcriptional silencing. This represents a remarkable example showcasing the functional coherence of independently evolved lncRNAs in distantly related mammalian lineages.
Agrin-Lrp4 pathway in hippocampal astrocytes restrains development of temporal lobe epilepsy through adenosine signaling
Authors: Liu Zi-Yang , Li Yuan-Quan , Wang Die-Lin , Wang Ying , Qiu Wan-Ting , Qiu Yu-Yang , Zhang He-Lin , You Qiang-Long , Liu Shi-min , Liang Qiu-Ni , Wu Er-Jian , Hu Bing-Jie , Sun Xiang-Dong 
Published: May 2024

AbstractBackgroundHuman patients often experience an episode of serious seizure activity, such as status epilepticus (SE), prior to the onset of temporal lobe epilepsy (TLE), suggesting that SE can trigger the development of epilepsy. Yet, the underlying mechanisms are not fully understood. The low-density lipoprotein receptor related protein (Lrp4), a receptor for proteoglycan-agrin, has been indicated to modulate seizure susceptibility. However, whether agrin-Lrp4 pathway also plays a role in the development of SE-induced TLE is not clear.MethodsLrp4f/fmice were crossed withhGFAP-CreandNex-Cremice to generate brain conditional Lrp4 knockout mice (hGFAP-Lrp4−/−) and pyramidal neuron specific knockout mice (Nex-Lrp4−/−). Lrp4 was specifically knocked down in hippocampal astrocytes by injecting AAV virus carrying hGFAP-Cre into the hippocampus. The effects of agrin-Lrp4 pathway on the development of SE-induced TLE were evaluated on the chronic seizure model generated by injecting kainic acid (KA) into the amygdala. The spontaneous recurrent seizures (SRS) in mice were video monitored.ResultsWe found thatLrp4deletion from the brain but not from the pyramidal neurons elevated the seizure threshold and reduced SRS numbers, with no change in the stage or duration of SRS. More importantly, knockdown of Lrp4 in the hippocampal astrocytes after SE induction decreased SRS numbers. In accord, direct injection of agrin into the lateral ventricle of control mice but not mice withLrp4deletion in hippocampal astrocytes also increased the SRS numbers. These results indicate a promoting effect of agrin-Lrp4 signaling in hippocampal astrocytes on the development of SE-induced TLE. Last, we observed that knockdown of Lrp4 in hippocampal astrocytes increased the extracellular adenosine levels in the hippocampus 2 weeks after SE induction. Blockade of adenosine A1 receptor in the hippocampus by DPCPX after SE induction diminished the effects of Lrp4 on the development of SE-induced TLE.ConclusionThese results demonstrate a promoting role of agrin-Lrp4 signaling in hippocampal astrocytes in the development of SE-induced development of epilepsy through elevating adenosine levels. Targeting agrin-Lrp4 signaling may serve as a potential therapeutic intervention strategy to treat TLE.
Information and communication technology in SMEs: a systematic literature review
Authors: Yuwono Tri , Suroso Agus , Novandari Weni 
Published: May 2024

AbstractThe adoption of information and communication technology (ICT) in small- and medium-sized firms (SMEs) is steadily increasing. However, the implementation of ICT in SMEs is hindered by constraints, such as limited financial resources, lack of ICT knowledge, and reluctance to change. The objective of this study is to identify and analyze prevalent patterns, obstacles and influences that impact the adoption of ICT in SMEs in developing nations. In addition, this study aims to provide recommendations on the most effective methods to optimize the utilization of ICT in these contexts. This review is conducted using a sample of 25 publications that have been published and are available in the Scopus database. The selection process focuses on works that have received significant citations and have been published between 2014 and 2023. The PRISMA statement form was utilized to elucidate the comprehensive procedure of selecting and accepting pertinent publications. This research makes two distinct contributions. The writers begin by doing a thorough literature analysis to examine the progress of ICT in the past decade. They next emphasize noteworthy research discoveries in this field. Furthermore, the authors give a comprehensive analysis of the ICT literature by quantifying yearly publications and identifying SMEs that primarily utilize ICT, academic journals that publish pertinent articles, and ICT-related publications across various fields and areas in developing nations. The study also discloses the papers, journals, and authors that have received the highest number of citations. The author's final argument in the paper is that, despite the extensive research conducted in the field of ICT, there is a necessity to customize strategies and policies that can support SMEs in the ICT sector, particularly in developing countries.
Sgrgan: sketch-guided restoration for traditional Chinese landscape paintings
Authors: Hu Qiyao , Huang Weilu , Luo Yinyin , Cao Rui , Peng Xianlin , Peng Jinye , Fan Jianping 
Published: May 2024

Abstract
Image restoration is a prominent field of research in computer vision. Restoring broken paintings, especially ancient Chinese artworks, is a significant challenge for current restoration models. The difficulty lies in realistically reinstating the intricate and delicate textures inherent in the original pieces. This process requires preserving the unique style and artistic characteristics of the ancient Chinese paintings. To enhance the effectiveness of restoring and preserving traditional Chinese paintings, this paper presents a framework called Sketch-Guided Restoration Generative Adversarial Network, termd SGRGAN. The framework employs sketch images as structural priors, providing essential information for the restoration process. Additionally, a novel Focal block is proposed to enhance the fusion and interaction of textural and structural elements. It is noteworthy that a BiSCCFormer block, incorporating a Bi-level routing attention mechanism, is devised to comprehensively grasp the structural and semantic details of the image, including its contours and layout. Extensive experiments and ablation studies on MaskCLP and Mural datasets demonstrate the superiority of the proposed method over previous state-of-the-art methods. Specifically, the model demonstrates outstanding visual fidelity, particularly in the restoration of landscape paintings. This further underscores its efficacy and universality in the realm of cultural heritage preservation and restoration.
Exploring the potential of nanomaterials (NMs) as diagnostic tools and disease resistance for crop pathogens
Authors: Jabran Muhammad , Ali Muhammad Amjad , Muzammil Saima , Zahoor Adil , Ali Faizan , Hussain Sarfaraz , Muhae-Ud-Din Ghulam , Ijaz Munazza , Gao Li 
Published: May 2024

AbstractFood crops are attacked by microbial pathogens and insect pests, leading to significant yield reductions and economic losses. Conventional disease diagnosis and management approaches often fail to provide rapid and eco-friendly solutions. In the current situation, nanomaterials (NMs) serve a valuable role in both managing emerging pathogens and monitoring overall plant health. Nanotechnology has transformed the biotechnology industry including agriculture with specific applications such as nano-fungicides, nano-bactericides, and nano-pesticides. This review focuses on the use of various nanomaterials, including inorganic materials such as Ag, ZnO, CuO, and CeO, as well as carbon-based nanoparticles, nanotubes, nanowires, and nano-capsules. The application of NMs holds the potential to address various challenges in food security through novel applications like advanced nano-biosensors for rapid pathogen detection and targeted disease management strategies. This includes the potential to minimize reliance on chemical inputs and contribute to more sustainable agricultural practices. Nanomaterials (NMs) promise to deliver plant hormones and signaling molecules to plants, enhancing resistance inducers against major crop pathogens. NMs against newly arising pathogens through reactive oxygen generation, membrane damage, and biochemical interference are also reviewed. However, challenges regarding the stability, toxicity, and environmental impacts of NMs are discussed, along with recommendations on green synthesis and functionalization approaches. This article aims to investigate the role of nanomaterials (NMs) in managing emerging pathogens and monitoring overall crop health offering an insightful outlook for future generations. Further biosafety aspects and larger-scale validation of NM-based applications could enable their commercialization for improving global food security.
Graphical Abstract
Connexin channels and hemichannels are modulated differently by charge reversal at residues forming the intracellular pocket
Authors: Villanelo Felipe , Minogue Peter J. , Maripillán Jaime , Reyna-Jeldes Mauricio , Jensen-Flores Joaquin , García Isaac E. , Beyer Eric C. , Pérez-Acle Tomás , Berthoud Viviana M. , Martínez Agustín D. 
Published: May 2024

Abstract
Background
Members of the β-subfamily of connexins contain an intracellular pocket surrounded by amino acid residues from the four transmembrane helices. The presence of this pocket has not previously been investigated in members of the α-, γ-, δ-, and ε-subfamilies. We studied connexin50 (Cx50) as a representative of the α-subfamily, because its structure has been determined and mutations of Cx50 are among the most common genetic causes of congenital cataracts.

Methods
To investigate the presence and function of the intracellular pocket in Cx50 we used molecular dynamics simulation, site-directed mutagenesis, gap junction tracer intercellular transfer, and hemichannel activity detected by electrophysiology and by permeation of charged molecules.

Results
Employing molecular dynamics, we determined the presence of the intracellular pocket in Cx50 hemichannels and identified the amino acids participating in its formation. We utilized site-directed mutagenesis to alter a salt-bridge interaction that supports the intracellular pocket and occurs between two residues highly conserved in the connexin family, R33 and E162. Substitution of opposite charges at either position decreased formation of gap junctional plaques and cell–cell communication and modestly reduced hemichannel currents. Simultaneous charge reversal at these positions produced plaque-forming non-functional gap junction channels with highly active hemichannels.

Conclusions
These results show that interactions within the intracellular pocket influence both gap junction channel and hemichannel functions. Disruption of these interactions may be responsible for diseases associated with mutations at these positions.