bluuebunny/crossref_metadata_2025_split huggingface dataset:
Water Vapor Measurements by Howard University Raman Lidar during the WAVES 2006 Campaign
Authors: Adam M. , Demoz B. B. , Venable D. D. , Joseph E. , Connell R. , Whiteman D. N. , Gambacorta A. , Wei J. , Shephard M. W. , Miloshevich L. M. , Barnet C. D. , Herman R. L. , Fitzgibbon J. 
Published: September 2009

Abstract
Water vapor mixing ratio retrieval using the Howard University Raman lidar is presented with emphasis on three aspects: (i) comparison of the lidar with collocated radiosondes and Raman lidar, (ii) investigation of the relationship between atmospheric state variables and the relative performance of the lidar and sonde (in particular, their poor agreement), and (iii) comparison with satellite-based measurements. The measurements were acquired during the Water Vapor Validation Experiment Sondes/Satellites 2006 campaign. Ensemble averaging of water vapor mixing ratio data from 10 nighttime comparisons with Vaisala RS92 radiosondes shows, on average, an agreement within ±10%, up to ∼8 km. A similar analysis of lidar-to-lidar data of over 700 profiles revealed an agreement to within 20% over the first 7 km (10% below 4 km). A grid analysis, defined in the temperature–relative humidity space, was developed to characterize the lidar–radiosonde agreement and quantitatively localizes regions of strong and weak correlations as a function of altitude, temperature, or relative humidity. Three main regions of weak correlation emerge: (i) regions of low relative humidity and low temperature, (ii) regions of moderate relative humidity at low temperatures, and (iii) regions of low relative humidity at moderate temperatures. Comparison of Atmospheric Infrared Sounder and Tropospheric Emission Sounder satellite retrievals of moisture with those of Howard University Raman lidar showed a general agreement in the trend, but the satellites miss details in atmospheric structure because of their low resolution. A relative difference of about ±20% is usually found between lidar and satellite measurements for the coincidences available.
On the Origin of Atmospheric Frontal Lines off the East Coast of Taiwan Observed on Spaceborne Synthetic Aperture Radar Images
Authors: Alpers Werner , Chen Jen-Ping , Pi Chia-Jung , Lin I-I. 
Published: September 2009

Abstract
Frontal lines having offshore distances typically between 40 and 80 km are often visible on synthetic aperture radar (SAR) images acquired over the east coast of Taiwan by the European Remote Sensing Satellites 1 and 2 (ERS-1 and ERS-2) and Envisat. In a previous paper the authors showed that they are of atmospheric and not of oceanic origin; however, in that paper they did not give a definite answer to the question of which physical mechanism causes them. In this paper the authors present simulations carried out with the fifth-generation Pennsylvania State University–National Center for Atmospheric Research Mesoscale Model, which shows that the frontal lines are associated with a quasi-stationary low-level convergence zone generated by the dynamic interaction of onshore airflow of the synoptic-scale wind with the coastal mountain range of the island of Taiwan. Reversed airflow collides with the onshore-flowing air leading to an uplift of air, which is often accompanied by the formation of bands of increased cloud density and of rainbands. The physical mechanism causing the generation of the frontal lines is similar to the one responsible for the formation of cloud bands off the Island of Hawaii as described by Smolarkiewicz et al. Four SAR images are shown, one acquired by ERS-2 and three by Envisat, showing frontal lines at the east coast of Taiwan caused by this generation mechanism. For these events the recirculation pattern, as well as the frontal (or convective) lines observed, were reproduced quite well with the meteorological model. So, it is argued that the observed frontal lines are not seaward boundaries of (classical) barrier jets or of katabatic wind fields, which have characteristics that are quite different from the flow patterns around the east coast of Taiwan as indicated by the SAR images.
Tornadoes without NWS Warning
Authors: Brotzge J. , Erickson S. 
Published: September 2009

Abstract
During a 5-yr period of study from 2000 to 2004, slightly more than 26% of all reported tornadoes across the United States occurred without an NWS warning being issued. This study examines some of the reasons behind why no warnings were issued with these tornadoes, and what climatological, storm classification, and sociological factors may have played a role in the lack of warnings. This dataset of tornado records was sorted by F scale, geographically by region and weather forecast office (WFO), hour of the day, month of the year, tornado pathlength, tornado-to-radar distance, county population density, and number of tornadoes by day and order of occurrence.
Results show that the tornadoes most likely to strike when the public is least likely to be aware were also those tornadoes with the greatest chance of not being warned. Singular tornado events (one tornado report per day within a WFO county warning area) and the first tornado report of the day were the most difficult scenarios on which to warn, with over half of all solitary tornado events not warned. Geographic areas that experienced a significant proportion of weak, solitary, and/or nocturnal tornadoes had a much higher ratio of missed warnings. In general, the stronger the tornado, as estimated from its F-scale rating and/or track length, the greater chance it was warned. However, the tornado distance from radar had a significant impact on tornado warning statistics. In addition, many weak tornadoes were not warned, and the overall ratio of missed tornado warnings to reported tornadoes actually increased over more densely populated regions, likely due to more complete postevent verification.
A new set of ESTs and cDNA clones from full-length and normalized libraries for gene discovery and functional characterization in citrus
Authors: Marques M Carmen , Alonso-Cantabrana Hugo , Forment Javier , Arribas Raquel , Alamar Santiago , Conejero Vicente , Perez-Amador Miguel A 
Published: September 2009

Abstract

Background
Interpretation of ever-increasing raw sequence information generated by modern genome sequencing technologies faces multiple challenges, such as gene function analysis and genome annotation. Indeed, nearly 40% of genes in plants encode proteins of unknown function. Functional characterization of these genes is one of the main challenges in modern biology. In this regard, the availability of full-length cDNA clones may fill in the gap created between sequence information and biological knowledge. Full-length cDNA clones facilitate functional analysis of the corresponding genes enabling manipulation of their expression in heterologous systems and the generation of a variety of tagged versions of the native protein. In addition, the development of full-length cDNA sequences has the power to improve the quality of genome annotation.

Results
We developed an integrated method to generate a new normalized EST collection enriched in full-length and rare transcripts of different citrus species from multiple tissues and developmental stages. We constructed a total of 15 cDNA libraries, from which we isolated 10,898 high-quality ESTs representing 6142 different genes. Percentages of redundancy and proportion of full-length clones range from 8 to 33, and 67 to 85, respectively, indicating good efficiency of the approach employed. The new EST collection adds 2113 new citrus ESTs, representing 1831 unigenes, to the collection of citrus genes available in the public databases. To facilitate functional analysis, cDNAs were introduced in a Gateway-based cloning vector for high-throughput functional analysis of genes in planta. Herein, we describe the technical methods used in the library construction, sequence analysis of clones and the overexpression of CitrSEP, a citrus homolog to the Arabidopsis SEP3 gene, in Arabidopsis as an example of a practical application of the engineered Gateway vector for functional analysis.

Conclusion
The new EST collection denotes an important step towards the identification of all genes in the citrus genome. Furthermore, public availability of the cDNA clones generated in this study, and not only their sequence, enables testing of the biological function of the genes represented in the collection. Expression of the citrus SEP3 homologue, CitrSEP, in Arabidopsis results in early flowering, along with other phenotypes resembling the over-expression of the Arabidopsis SEPALLATA genes. Our findings suggest that the members of the SEP gene family play similar roles in these quite distant plant species.
Antibody expressing pea seeds as fodder for prevention of gastrointestinal parasitic infections in chickens
Authors: Zimmermann Jana , Saalbach Isolde , Jahn Doreen , Giersberg Martin , Haehnel Sigrun , Wedel Julia , Macek Jeanette , Zoufal Karen , Glünder Gerhard , Falkenburg Dieter , Kipriyanov Sergey M 
Published: September 2009

AbstractBackgroundCoccidiosis caused by protozoans of genusEimeriais a chicken parasitic disease of great economical importance. Conventional disease control strategies depend on vaccination and prophylactic use of anticoccidial drugs. Alternative solution to prevent and treat coccidiosis could be provided by passive immunization using orally delivered neutralizing antibodies. We investigated the possibility to mitigate the parasitic infection by feeding poultry with antibody expressing transgenic crop seeds.ResultsUsing the phage display antibody library, we generated a panel of anti-EimeriascFv antibody fragments with high sporozoite-neutralizing activity. These antibodies were expressed either transiently in agrobacteria-infiltrated tobacco leaves or stably in seeds of transgenic pea plants. Comparison of the scFv antibodies purified either from tobacco leaves or from the pea seeds demonstrated no difference in their antigen-binding activity and molecular form compositions. Force-feeding experiments demonstrated that oral delivery of flour prepared from the transgenic pea seeds had higher parasite neutralizing activityin vivothan the purified antibody fragments isolated from tobacco. The pea seed content was found to protect antibodies against degradation by gastrointestinal proteases (>100-fold gain in stability).Ad libitumfeeding of chickens demonstrated that the transgenic seeds were well consumed and not shunned. Furthermore, feeding poultry with shred prepared from the antibody expressing pea seeds led to significant mitigation of infection caused both by high and low challenge doses ofEimeriaoocysts.ConclusionThe results suggest that our strategy offers a general approach to control parasitic infections in production animals using cost-effective antibody expression in crop seeds affordable for the animal health market.
Functional consequences of structural differences in stingray sensory systems. Part I: mechanosensory lateral line canals
Authors: Jordan Laura K. , Kajiura Stephen M. , Gordon Malcolm S. 
Published: September 2009

SUMMARY
Short range hydrodynamic and electrosensory signals are important during final stages of prey capture in elasmobranchs (sharks, skates and rays), and may be particularly useful for dorso-ventrally flattened batoids with mouths hidden from their eyes. In stingrays, both the lateral line canal and electrosensory systems are highly modified and complex with significant differences on ventral surfaces that relate to feeding ecology. This study tests functional hypotheses based on quantified differences in sensory system morphology of three stingray species, Urobatis halleri, Myliobatis californica and Pteroplatytrygon violacea. Part I investigates the mechanosensory lateral line canal system whereas part II focuses on the electrosensory system. Stingray lateral line canals include both pored and non-pored sections and differ in branching complexity and distribution. A greater proportion of pored canals and high pore numbers were predicted to correspond to increased response to water flow. Behavioral experiments were performed to compare responses of stingrays to weak water jets mimicking signals produced by potential prey at velocities of 10–20 cm s–1. Bat rays, M. californica, have the most complex and broadly distributed pored canal network and demonstrated both the highest response rate and greater response intensity to water jet signals. Results suggest that U. halleri and P. violacea may rely on additional sensory input, including tactile and visual cues, respectively, to initiate stronger feeding responses. These results suggest that stingray lateral line canal morphology can indicate detection capabilities through responsiveness to weak water jets.
Functional consequences of structural differences in stingray sensory systems. Part II: electrosensory system
Authors: Jordan Laura K. , Kajiura Stephen M. , Gordon Malcolm S. 
Published: September 2009

SUMMARY
Elasmobranch fishes (sharks, skates and rays) possess highly sensitive electrosensory systems, which enable them to detect weak electric fields such as those produced by potential prey organisms. Different species have unique electrosensory pore numbers, densities and distributions. Functional differences in detection capabilities resulting from these structural differences are largely unknown. Stingrays and other batoid fishes have eyes positioned on the opposite side of the body from the mouth. Furthermore, they often feed on buried prey, which can be located non-visually using the electrosensory system. In the present study we test functional predictions based on structural differences in three stingray species (Urobatis halleri, Pteroplatytrygon violacea and Myliobatis californica)with differing electrosensory system morphology. We compare detection capabilities based upon behavioral responses to dipole electric signals(5.3–9.6 μA). Species with greater ventral pore numbers and densities were predicted to demonstrate enhanced electrosensory capabilities. Electric field intensities at orientation were similar among these species, although they differed in response type and orientation pathway. Minimum voltage gradients eliciting feeding responses were well below 1 nVcm–1 for all species regardless of pore number and density.
Navigational abilities of adult and experienced homing pigeons deprived of olfactory or trigeminally mediated magnetic information
Authors: Gagliardo Anna , Ioalè Paolo , Savini Maria , Wild Martin 
Published: September 2009

SUMMARY
Anatomical evidence and conditioning experiments have suggested that magnetoreceptors innervated by the ophthalmic branch of the trigeminal nerve are located in the upper beak of homing pigeons. Following these findings it has been proposed that the trigeminally-mediated magnetorececeptors are able to detect magnetic field intensity, which might be useful for a position finding mechanism for pigeons homing from unfamiliar locations. Recent data have shown that, in inexperienced pigeons, section of the ophthalmic branch of the trigeminal nerve does not impair navigational abilities. Similarly, no impairment was observed if the trigeminal section was performed on young pigeons, before they have had the opportunity to learn a navigational map. By contrast, section of the olfactory nerve either in adult inexperienced pigeons or in young birds before map learning, disrupted their homing performance. Nevertheless, because a magnetic map mechanism requires training flights for learning the magnetic gradient of the territory around the loft, the question remains as to whether the navigational performance of adult experienced pigeons can be affected by lack of magnetic information. To answer this question we extensively group-trained adult pigeons and then surgically deprived them of either olfactory or trigeminally mediated magnetic information, prior to testing their navigational abilities. The birds deprived of trigeminally mediated magnetic information displayed similar navigational abilities as intact control pigeons, whereas the olfactory-deprived pigeons were dramatically impaired in homing. Our data show that even in trained adult pigeons, olfactory cues are needed for homing from unfamiliar locations and that the lack of magnetic information does not affect navigational abilities of experienced adult homing pigeons.
Endocrine mechanisms mediating temperature-induced reproductive behavior in red-sided garter snakes (Thamnophis sirtalis parietalis)
Authors: Lutterschmidt Deborah I. , Mason Robert T. 
Published: September 2009

SUMMARY
We investigated the mechanisms by which temperature induces seasonal reproductive behavior in red-sided garter snakes (Thamnophis sirtalis parietalis). Specifically, we addressed whether elevated temperatures during winter dormancy influence (1) diel melatonin and corticosterone rhythms; (2) sex steroid hormone and corticosterone profiles; and (3) the expression of reproductive behavior following emergence. Elevated hibernation temperatures (i.e. 10°C versus 5°C) significantly increased overall melatonin and decreased corticosterone concentrations of snakes. The temperature-induced differences in melatonin rhythms between the 5°C and 10°C treatment groups persisted even after both groups were again acclimated to 10°C, indicating that cold temperature exposure has a lasting influence on melatonin rhythms. Elevated hibernation temperatures also significantly altered androgen and corticosterone profiles of snakes,providing a potential mechanism to explain reported annual variation in steroid hormones. Although previous studies indicate that male red-sided garter snakes exhibit a dissociated reproductive strategy, we demonstrate the presence of intersexual variation in sex steroid hormone profiles, as estradiol concentrations of female snakes increased significantly prior to spring mating activity. Importantly, the percentage change in body mass did not differ significantly between snakes in the hibernation treatments,indicating that the observed changes in hormone profiles are indeed temperature induced and not simply an indirect result of significant changes in the energy balance of snakes. Finally, in males maintained at 10°C during winter dormancy the onset of courtship behavior following emergence was delayed. Our results suggest that environmental temperatures induce reproductive behavior, in part, via changes in diel melatonin and/or corticosterone rhythms in this seasonally breeding reptile.
Functional differences between two CRF-related diuretic hormone receptors in Drosophila
Authors: Hector Clare E. , Bretz Colin A. , Zhao Yan , Johnson Erik C. 
Published: September 2009

SUMMARY
In Drosophila, two related G-protein-coupled receptors are members of the corticotropin releasing factor (CRF) receptor subfamily. We have previously reported that one of these receptors, encoded by CG8422 is a functional receptor for a diuretic hormone, DH44. Here, we report that the other CRF receptor subfamily member, encoded by CG12370, is also a receptor for the DH44 neuropeptide. The lines of evidence to support this identification include increases in cAMP levels due to CG12370 receptor activation and the recruitment of β-arrestin–GFP to the plasma membrane in response to DH44 application. We compared these features of the receptors DH44-R2 (encoded by CG12370) and DH44-R1(encoded by CG8422) and found fundamental differences in signaling,association with the arrestins, and peptide sensitivity. We found that the sensitivity of DH44-R2 to the DH44 peptide is lower than that of DH44-R1, specifically an estimated EC50 of 7.98E-07 moll–1 for DH44 by DH44-R2 to an EC50of 5.12E-09 moll–1 by DH44-R1 and found that previous reports on the sensitivity of the tubule to DH44 is in agreement with our measurements of DH44-R2 sensitivity. We employed a specific RNAi construct to selectively knock-down DH44-R2 expression and this led to heightened sensitivity to osmotic challenges. The functional characterization of this diuretic hormone receptor in Drosophila demonstrates a high degree of conservation of CRF-like signaling.
Electrochemically Modulated Film Permeability: A Functional Film for Controlled Reagent Release
Authors: Sahni Himanshu , Villalba Maria Marti , Newton Laura A A , Leslie Ray , Davis James 
Published: September 2009

Abstract
The electrochemical transformation of an insoluble quinone layer has been investigated as the basis of a smart material and its applicability for reagent delivery applications has been assessed. The quinone is shown to form a protective layer whose permeability and susceptibility toward dissolution can be modulated through the imposition of an appropriate electrochemical trigger.
Syntheses and Photochromic Properties of Metacyclothiophenophan-1-enes
Authors: Takeshita Michinori , Jin-nouchi Hirotsugu , Ishikawa Jun 
Published: September 2009

Abstract
A small thia[3]metacyclo[2]thiophenophan-1-ene, cyclo-phane composed of a thiophene ring, a benzene ring, and their bridge, has been synthesized and displays photochromism, while a metacyclothiophenophan-1-ene bearing a long bridge was non-photochromic due to little conjugation between three double bonds, that was estimated by DFT calculations.
Oral Fluid Testing for Drugs of Abuse
Authors: Bosker Wendy M , Huestis Marilyn A 
Published: September 2009

AbstractBackground: Oral fluid (OF) is an exciting alternative matrix for monitoring drugs of abuse in workplace, clinical toxicology, criminal justice, and driving under the influence of drugs (DUID) programs. During the last 5 years, scientific and technological advances in OF collection, point-of-collection testing devices, and screening and confirmation methods were achieved. Guidelines were proposed for workplace OF testing by the Substance Abuse and Mental Health Services Administration, DUID testing by the European Union’s Driving under the Influence of Drugs, Alcohol and Medicines (DRUID) program, and standardization of DUID research. Although OF testing is now commonplace in many monitoring programs, the greatest current limitation is the scarcity of controlled drug administration studies available to guide interpretation.Content: This review outlines OF testing advantages and limitations, and the progress in OF that has occurred during the last 5 years in collection, screening, confirmation, and interpretation of cannabinoids, opioids, amphetamines, cocaine, and benzodiazepines. We examine controlled drug administration studies, immunoassay and chromatographic methods, collection devices, point-of-collection testing device performance, and recent applications of OF testing.Summary: Substance Abuse and Mental Health Services Administration approval of OF testing was delayed because questions about drug OF disposition were not yet resolved, and collection device performance and testing assays required improvement. Here, we document the many advances achieved in the use of OF. Additional research is needed to identify new biomarkers, determine drug detection windows, characterize OF adulteration techniques, and evaluate analyte stability. Nevertheless, there is no doubt that OF offers multiple advantages as an alternative matrix for drug monitoring and has an important role in DUID, treatment, workplace, and criminal justice programs.