bluuebunny/crossref_metadata_2025_split huggingface dataset:
Skeletal changes mimicking intrathoracic disease on chest radiographs
Authors: Van Gelderen WFC 
Published: November 2009

SummaryVarious chest radiographs are illustrated to demonstrate features where bony changes may mimic intrathoracic disease. To confirm the skeletal origin and nature, further conventional radiographs often suffice, and the need for CT or scintigraphy may therefore be obviated. At the time of presentation for radiography of the chest, further pertinent clinical details can be obtained from the patient by the department staff, as required. All previous radiographs and radiological reports should be readily available. In order to add to the educational value of the 13 cases illustrated, all are presented in quiz format, with the answers and further images included in the text.
Locally invasive nodular lymphoid hyperplasia: Radiological findings in a case of pulmonary and sinus disease
Authors: Siemienowicz M , Simkin P , Straub A , House R 
Published: November 2009

SummaryNodular lymphoid hyperplasia is a rare, benign, lymphoproliferative disease usually found in the gastrointestinal tract. It has never been reported in the sinuses. We present an unusual case of pulmonary nodular lymphoid hyperplasia with locally invasive sinus involvement and describe the CT, MRI and positron emission tomography findings.
Photodynamic therapy for chronic central serous chorioretinopathy
Authors: Ruiz‐Moreno José M. , Lugo Francisco L. , Armadá Felix , Silva Rufino , Montero Javier A. , Arevalo J. Fernando , Arias Luis , Gómez‐Ulla Francisco 
Published: November 2009

Abstract.Purpose:  This study aimed to evaluate the efficacy of photodynamic therapy (PDT) in treating chronic central serous chorioretinopathy (CSC).Methods:  We describe a non‐randomized, multicentre, interventional case series. A total of 82 eyes of 72 patients with chronic CSC were treated by conventional PDT. LogMAR best corrected visual acuity (BCVA) (ETDRS charts) and central foveal thickness (CFT) measured by optical coherence tomography before and after PDT, number of PDT treatments and complications were used as outcome indicators.Results:  Mean follow‐up was 12 ± 10 months and mean age was 46 ± 10 years. Mean logMAR BCVA changed from 0.53 (standard deviation [SD] 0.43) before PDT to 0.38 (SD 0.41) at 3 months and 0.48 (SD 0.50) at 6 months (p < 0.0001 and p = 0.007, respectively, Student’s t‐test for paired data). Mean BCVA at the end of follow‐up was 0.37 (SD 0.45; p < 0.0001 from baseline). Macular detachment was resolved and subretinal fluid (SRF) disappeared in all cases. Central foveal thickness decreased from 325 μm (SD 95), to 229 μm (SD 70) at 1 month after PDT, 206 μm (SD 68) at 3 months, and 202 μm (SD 76) at 6 months (all p < 0.0001, Student’s t‐test for paired data). No cases developed severe visual loss or complications derived from PDT. Reactive retinal pigment epithelium hypertrophy appeared in nine cases after PDT.Conclusions:  Photodynamic therapy with verteporfin may be useful in chronic CSC for improving BCVA and reducing SRF and CFT. Randomized studies with longer follow‐up are needed to assess the real role of this treatment in chronic CSC.
Detection and characterisation of β-globin gene cluster deletions in Chinese using multiplex ligation-dependent probe amplification
Authors: So C C , So A C Y , Chan A Y Y , Tsang S T Y , Ma E S K , Chan L C 
Published: November 2009

Background:Deletions in the β-globin cluster causing thalassaemia and hereditary persistence of fetal haemoglobin (HPFH) are uncommon and difficult to detect. Data in Chinese are very scarce.Aims:To use a recently available technique to investigate the frequencies and nature of β-globin cluster deletions in Chinese.Methods:106 subjects with phenotypes of thalassaemia or HPFH and suspected to have deletions in the β-globin cluster were studied. A commercially available kit employing multiplex ligation-dependent probe amplification (MLPA) was used to screen for deletions. Gap PCR and direct nucleotide sequencing were used to characterise deletions detected.Results:17 deletions in the β-globin cluster were found in 17 patients: 8 of Chinese (Aγδβ)0thalassaemia, 7 of Southeast Asian (Vietnamese) deletion and 2 of Thai (Aγδβ)0thalassaemia. The only type of deletion detected in δβ-thalassaemia was Chinese (Aγδβ)0thalassaemia. The deletional form of HPFH was rarely seen in only 1 case of Thai (Aγδβ)0thalassaemia. Deletions presenting as β-thalassaemia trait and raised HbF were all of the Southeast Asian (Vietnamese) deletion type. When these deletions were co-inherited with classical β-thalassaemia mutations in compound heterozygous states, the phenotypes could be very variable.Conclusions:In the Chinese population, there are only relatively few types of deletions seen in the β-globin cluster. MLPA is a fast and effective way of screening for these deletions. Characterisation of these deletions allows the development of simpler and more specific PCR-based tests for routine diagnostic use. Accurate prediction of phenotype is not always feasible. The molecular defects in many cases of HPFH still await discovery.
CD24 shows early upregulation and nuclear expression but is not a prognostic marker in colorectal cancer
Authors: Ahmed M A H , Al-Attar A , Kim J , Watson N F S , Scholefield J H , Durrant L G , Ilyas M 
Published: November 2009

Background and aims:The putative stem cell marker CD24 is a small, heavily glycosylated, cell surface molecule which was originally associated with tumour metastasis. Recently it has been reported to be upregulated and of prognostic importance in colorectal tumours. The study aims to study the prognostic value of CD24 in a large series of colorectal cancer (CRC).Methods:CD24 protein expression was examined by immunohistochemistry. A total of 10 whole tissue sections (WTS) of adenoma and 345 CRCs arranged as tissue microarrays (TMAs) were evaluated. For comparison with non-neoplastic tissue, 10 WTS containing tumour with associated non-neoplastic tissue were also studied.Results:None of the samples of normal tissue (adjacent to tumour) showed CD24 expression. In the tumours, CD24 expression was seen on the luminal surface of the cells, within the cytoplasm and, unexpectedly, also within the nucleus. Positive immunostaining was seen in 9/10 (90%) adenomas and 313/345 (91%) of CRCs. Weak statistical associations were found between CD24 expression and some clinicopathological features. In contrast to other published studies, however, the analysis did not show any association between CD24 expression and poor prognosis—if anything it was found that loss of CD24 expression appeared to be more related to poor outcome.Conclusion:Upregulation of CD24 is an early and common event during the development of CRC and it may be expressed in any cellular compartment, including the nucleus. CD24 is not, however, a good prognostic marker in CRC.
The Detection of Early Atherosclerosis in Healthy Male Relatives of Men with Peripheral Arterial Disease: a Feasibility Study
Authors: Wolstenhulme Stephen , Ricci Stefano , West Robert M , Anthony Evans J , Julian A Scott D 
Published: November 2009

Aim: A feasibility study to attempt to determine if conventional ultrasound and multi-gate ultrasound could differentiate between men with peripheral arterial disease (PAD), healthy first-degree male relatives (FDRs) of men with PAD, and age/sex matched controls in identifying early atherosclerosis. Methods: three groups were studied using conventional ultrasound: first, four men with premature PAD (≤65 years); second, four healthy first-degree relatives; third, seven male controls with no known family history or evidence of premature cardiovascular disease. Primary outcome measures were plaque and intima-media thickness of the common carotid artery, flow mediated vasodilatation of the brachial artery, and lower extremity arterial plaque. Multigate Doppler ultrasound blood velocity profile measurements were obtained for three controls, but this series of measurements was discontinued due to technical difficulties. Statistics: mean (standard deviation) and analysis of variance (ANOVA) ( p<0·05) Results: Conventional ultrasound demonstrated lower extremity arterial lesions in all four of the patients with PAD. One of their first-degree relatives was found to have a non-significant lesion. No significant carotid artery lesions were found in any of the patients, but males with PAD had significantly greater carotid artery intima-media thickness than a combined group of first-degree relatives and controls (0·75±0·06 mm, versus 0·56±0·07 mm; p≤0·01). The mean (standard deviation) flow mediated vasodilation per cent change of the brachial artery was for males with PAD, 3·49±4·15; relatives, 6·17±2·53; and control subjects, 3·53±6·63. Results from the multigate system were inconclusive. Conclusion: Carotid artery intima-media thickness seemed able to differentiate between patients with PAD and the other two groups. This measurement could be used in future case-control family PAD studies and possibly eventually as a screening tool. Flow mediated dilatation per cent change showed a wide variation in measurements and was not able to differentiate between the three groups. For researchers considering future studies, it is worth noting that recruitment figures for this pilot study were much lower than quoted in previous reports.
High-throughput single nucleotide polymorphism genotyping using nanofluidic Dynamic Arrays
Authors: Wang Jun , Lin Min , Crenshaw Andrew , Hutchinson Amy , Hicks Belynda , Yeager Meredith , Berndt Sonja , Huang Wen-Yi , Hayes Richard B , Chanock Stephen J , Jones Robert C , Ramakrishnan Ramesh 
Published: November 2009

Abstract

Background
Single nucleotide polymorphisms (SNPs) have emerged as the genetic marker of choice for mapping disease loci and candidate gene association studies, because of their high density and relatively even distribution in the human genomes. There is a need for systems allowing medium multiplexing (ten to hundreds of SNPs) with high throughput, which can efficiently and cost-effectively generate genotypes for a very large sample set (thousands of individuals). Methods that are flexible, fast, accurate and cost-effective are urgently needed. This is also important for those who work on high throughput genotyping in non-model systems where off-the-shelf assays are not available and a flexible platform is needed.

Results
We demonstrate the use of a nanofluidic Integrated Fluidic Circuit (IFC) - based genotyping system for medium-throughput multiplexing known as the Dynamic Array, by genotyping 994 individual human DNA samples on 47 different SNP assays, using nanoliter volumes of reagents. Call rates of greater than 99.5% and call accuracies of greater than 99.8% were achieved from our study, which demonstrates that this is a formidable genotyping platform. The experimental set up is very simple, with a time-to-result for each sample of about 3 hours.

Conclusion
Our results demonstrate that the Dynamic Array is an excellent genotyping system for medium-throughput multiplexing (30-300 SNPs), which is simple to use and combines rapid throughput with excellent call rates, high concordance and low cost. The exceptional call rates and call accuracy obtained may be of particular interest to those working on validation and replication of genome- wide- association (GWA) studies.
Go contributes to olfactory reception in Drosophila melanogaster
Authors: Chatterjee Abhishek , Roman Gregg , Hardin Paul E 
Published: November 2009

Abstract

Background
Seven-transmembrane receptors typically mediate olfactory signal transduction by coupling to G-proteins. Although insect odorant receptors have seven transmembrane domains like G-protein coupled receptors, they have an inverted membrane topology and function as ligand-gated cation channels. Consequently, the involvement of cyclic nucleotides and G proteins in insect odor reception is controversial. Since the heterotrimeric Goα subunit is expressed in Drosophila olfactory receptor neurons, we reasoned that Go acts together with insect odorant receptor cation channels to mediate odor-induced physiological responses.

Results
To test whether Go dependent signaling is involved in mediating olfactory responses in Drosophila, we analyzed electroantennogram and single-sensillum recording from flies that conditionally express pertussis toxin, a specific inhibitor of Go in Drosophila. Pertussis toxin expression in olfactory receptor neurons reversibly reduced the amplitude and hastened the termination of electroantennogram responses induced by ethyl acetate. The frequency of odor-induced spike firing from individual sensory neurons was also reduced by pertussis toxin. These results demonstrate that Go signaling is involved in increasing sensitivity of olfactory physiology in Drosophila. The effect of pertussis toxin was independent of odorant identity and intensity, indicating a generalized involvement of Go in olfactory reception.

Conclusion
These results demonstrate that Go is required for maximal physiological responses to multiple odorants in Drosophila, and suggest that OR channel function and G-protein signaling are required for optimal physiological responses to odors.
Enhanced visual fields in hammerhead sharks
Authors: McComb D. M. , Tricas T. C. , Kajiura S. M. 
Published: November 2009

SUMMARYSeveral factors that influence the evolution of the unusual head morphology of hammerhead sharks (family Sphyrnidae) are proposed but few are empirically tested. In this study we tested the ‘enhanced binocular field’ hypothesis (that proposes enhanced frontal binocularity) by comparison of the visual fields of three hammerhead species: the bonnethead shark, Sphyrna tiburo, the scalloped hammerhead shark, Sphyrna lewini, and the winghead shark, Eusphyra blochii, with that of two carcharhinid species: the lemon shark, Negaprion brevirostris, and the blacknose shark, Carcharhinus acronotus. Additionally, eye rotation and head yaw were quantified to determine if species compensate for large blind areas anterior to the head. The winghead shark possessed the largest anterior binocular overlap (48 deg.) and was nearly four times larger than that of the lemon (10 deg.) and blacknose (11 deg.) sharks. The binocular overlap in the scalloped hammerhead sharks (34 deg.) was greater than the bonnethead sharks (13 deg.) and carcharhinid species; however, the bonnethead shark did not differ from the carcharhinids. These results indicate that binocular overlap has increased with lateral head expansion in hammerhead sharks. The hammerhead species did not demonstrate greater eye rotation in the anterior or posterior direction. However, both the scalloped hammerhead and bonnethead sharks exhibited greater head yaw during swimming (16.9 deg. and 15.6 deg., respectively) than the lemon (15.1 deg.) and blacknose (15.0 deg.) sharks, indicating a behavioral compensation for the anterior blind area. This study illustrates the larger binocular overlap in hammerhead species relative to their carcharhinid sister taxa and is consistent with the ‘enhanced binocular field’ hypothesis.
Acoustical mimicry in a predatory social parasite of ants
Authors: Barbero F. , Bonelli S. , Thomas J. A. , Balletto E. , Schönrogge K. 
Published: November 2009

SUMMARYRapid, effective communication between colony members is a key attribute that enables ants to live in dominant, fiercely protected societies. Their signals, however, may be mimicked by other insects that coexist as commensals with ants or interact with them as mutualists or social parasites. We consider the role of acoustics in ant communication and its exploitation by social parasites. Social parasitism has been studied mainly in the butterfly genus Maculinea, the final instar larvae of which are host-specific parasites of Myrmica ants, preying either on ant grubs (predatory Maculinea) or being fed by trophallaxis (cuckoo Maculinea). We found similar significant differences between the stridulations of model queen and worker ant castes in both Myrmica sabuleti and Myrmica scabrinodis to that previously reported for Myrmica schencki. However, the sounds made by queens of all three Myrmica species were indistinguishable, and among workers, stridulations did not differ significantly in two of three species-pairs tested. Sounds recorded from the predatory caterpillars and pupae of Maculinea arion had similar or closer patterns to the acoustics of their host Myrmica sabuleti than those previously reported for the cuckoo Maculinea rebeli and its host Myrmica schencki, even though Maculinea rebeli caterpillars live more intimately with their host. We conclude that chemical mimicry enables Maculinea larvae to be accepted as colony members by worker ants, but that caterpillars and pupae of both predatory and cuckoo butterflies employ acoustical mimicry of queen ant calls to elevate their status towards the highest attainable position within their host's social hierarchy.
The next step in cicada audition: measuring pico-mechanics in the cicada's ear
Authors: Windmill J. F. C. , Sueur J. , Robert D. 
Published: November 2009

SUMMARYFemale cicadas use sound when they select a mate from a chorus of singing males. The cicada has a tympanal ear; and the tympanal membrane, and constituent tympanal ridge, act as both acousto-mechanical transducers and frequency filters. The tympanal ridge is physically connected to a large number of mechanoreceptor neurons via a cuticular extension known as the tympanal apodeme. Using microscanning laser Doppler vibrometry, we measured for the first time the in vivo vibrations of the apodeme of female Cicadatra atra in response to the motion of the tympanum driven by sound. These measurements reveal that the nanoscale motion of the tympanal membrane is over a magnitude greater than that of the apodeme. Furthermore, the apodeme acts as an additional mechanical frequency filter, enhancing that of the tympanal ridge, narrowing the frequency band of vibration at the mechanoreceptor neurons to that of the male calling song. This study enhances our understanding of the mechanical link between the external ear of the cicada and its sensory cells.
Survival and DNA degradation in anhydrobiotic tardigrades
Authors: Rebecchi L. , Cesari M. , Altiero T. , Frigieri A. , Guidetti R. 
Published: November 2009

SUMMARY
Anhydrobiosis is a highly stable state of suspended animation in an organism due to its desiccation, which is followed by recovery after rehydration. Changes occurring during drying could damage molecules, including DNA. Using the anhydrobiotic tardigrade Paramacrobiotus richtersi as a model organism, we have evaluated the effects of environmental factors, such as temperature and air humidity level (RH), on the survival of desiccated animals and on the degradation of their DNA. Tardigrades naturally desiccated in leaf litter and tardigrades experimentally desiccated on blotting paper were considered. Replicates were kept at 37°C and at different levels of RH for 21 days. RH values and temperature, as well as time of exposure to these environmental factors, have a negative effect on tardigrade survival and on the time required by animals to recover active life after desiccation. DNA damages (revealed as single strand breaks) occurred only in desiccated tardigrades kept for a long time at high RH values. These results indicate that during the anhydrobiotic state, damages take place and accumulate with time. Two hypotheses can be formulated to explain the results: (i) oxidative damages occur in desiccated specimens of P. richtersi, and (ii) high temperatures and high RH values change the state of the disaccharide trehalose, reducing its protective role.
Testing the role of sensory systems in the migratory heading of a songbird
Authors: Holland R. A. , Thorup K. , Gagliardo A. , Bisson I. A. , Knecht E. , Mizrahi D. , Wikelski M. 
Published: November 2009

SUMMARYThe identification of the sensory cues and mechanisms by which migratory birds are able to reach the same breeding and wintering grounds year after year has eluded biologists despite more than 50 years of intensive study. While a number of environmental cues have been proposed to play a role in the navigation of birds, arguments still persist about which cues are essential for the experience based navigation shown by adult migrants. To date, few studies have tested the sensory basis of navigational cues used during actual migration in the wild: mainly laboratory based studies or homing during the non-migratory season have been used to investigate this behaviour. Here we tested the role of olfactory and magnetic cues in the migration of the catbird (Dumetella carolinensis) by radio tracking the migration of birds with sensory manipulations during their actual migratory flights. Our data suggest that adult birds treated with zinc sulphate to produce anosmia were unable to show the same orientation as control adults, and instead reverted to a direction similar to that shown by juveniles making their first migration. The magnetic manipulation had no effect on the orientation of either adults or juveniles. These results allow us to propose that the olfactory sense may play a role in experience based migration in adult catbirds. While the olfactory sense has been shown to play a role in the homing of pigeons and other birds, this is the first time it has been implicated in migratory orientation.
Sea turtles compensate deflection of heading at the sea surface during directional travel
Authors: Narazaki T. , Sato K. , Abernathy K. J. , Marshall G. J. , Miyazaki N. 
Published: November 2009

SUMMARYAir-breathing marine animals, including sea turtles, utilise two fundamentally different environments (i.e. sea surface and underwater) during migration. Many satellite telemetry studies have shown travel paths at relatively large spatio-temporal scales, discussing the orientation and navigation mechanisms that guide turtles. However, as travel paths obtained by satellite telemetry only reflect movements at the surface, little is known about movements and orientation ability underwater. In this study, to assess orientation ability both at the surface and underwater, fine-scale 3-D movements of free-ranging loggerhead turtles Caretta caretta were reconstructed by using multi-sensor data loggers. Video systems (‘Crittercam’) were also used to record the behaviour of the turtles and the visual information surrounding the turtles. During August and October in 2006 and 2007, eight turtles were released from Otsuchi Bay, Japan (39°20′30N, 141°56′00E), and a total of 118 h of 3-D movements were reconstructed. Turtles maintained highly straight-line courses (straightness index &gt;0.95) during 41% of the total duration (i.e. ‘travelling periods’). During travelling periods, turtles swam continuously, maintaining unidirectional heading throughout dives whereas turtles changed heading remarkably at the surface. Despite highly directional movements during dives, travel direction tended to shift by the end of dives lasting 10 minutes or more. Such deflections seemed to be compensated during subsequent surfacing periods because there was a negative relationship between changes in travel direction arising during dives and subsequent surfacing periods. Therefore, remarkable changes in heading at the surface could be interpreted as direction-searching behaviour. Our results suggested that turtles undertaking directional travel were more dependent on directional information that was reliable at the surface.