bluuebunny/crossref_metadata_2025_split huggingface dataset:
The longitudinal nonparametric test as a new tool to explore gene‐gene and gene‐time effects in cohorts
Authors: Malzahn D. , Schillert A. , Müller M. , Bickeböller H. 
Published: June 2010

AbstractCurrent approaches for analysis of longitudinal genetic epidemiological data of quantitative traits are typically restricted to normality assumptions of the trait. We introduce the longitudinal nonparametric test (LNPT) for cohorts with quantitative follow‐up data to test for overall main effects of genes and for gene‐gene and gene‐time interactions. The LNPT is a rank procedure and does not depend on normality assumptions of the trait. We demonstrate by simulations that the LNPT is powerful, keeps the type‐1 error level, and has very good small sample size behavior. For phenotypes with normal residuals, loss of power compared to parametric approaches (linear mixed models) was small for the quite general scenarios, which we simulated. For phenotypes with non‐normal residuals, gain in power by the LNPT can be substantial. In contrast to parametric approaches, the LNPT is invariant with respect to monotone transformations of the trait. It is mathematically valid for arbitrary trait distribution. Genet. Epidemiol. 34: 469‒478, 2010. © 2010 Wiley‐Liss, Inc.
BMI1 silencing enhances docetaxel activity and impairs antioxidant response in prostate cancer
Authors: Crea Francesco , Duhagon Serrat Maria A. , Hurt Elaine M. , Thomas Suneetha B. , Danesi Romano , Farrar William L. 
Published: June 2010

AbstractThe BMI1 oncogene promotes prostate cancer (PC) progression. High B‐cell‐specific Moloney murine leukemia virus integration site 1 (BMI1) expression predicts poor prognosis in PC patients. Recent evidence suggests that BMI1 may also play a role in docetaxel chemoresistance. However, mechanisms and clinical significance of BMI1‐related chemoresistance have not been investigated. For this purpose, BMI1 was silenced in 2 PC cell lines (LNCaP and DU 145). Cell proliferation and apoptosis after docetaxel treatment were measured. Guanine oxidation was assessed by in‐cell western. Global gene expression analysis was performed on BMI1 silenced cells. Oncomine database was used to compare in vitro data with gene expression in PC samples. BMI1 silencing had no effect on cell proliferation but significantly enhanced docetaxel‐induced antitumor activity. Gene expression analysis demonstrated that BMI1 silencing downregulates a set of antioxidant genes. Docetaxel treatment increased guanine oxidation, whereas the antioxidant N‐acetyl cysteine rescued docetaxel‐induced cell death. Examination of clinical datasets revealed a positive correlation of BMI1 and antioxidant gene expression. BMI1‐controlled antioxidant genes were predictive of poor prognosis in PC patients. In conclusion, BMI1 enhances antioxidant response, thereby allowing PC survival after docetaxel‐based chemotherapy. BMI1‐controlled antioxidant genes are overexpressed in aggressive PC and should be tested as predictors of chemotherapy failure.
Skeletal microstructural abnormalities in postmenopausal women with chronic obstructive pulmonary disease
Authors: Kulak Carolina AM , Borba Victoria C , Jorgetti Vanda , dos Reis Luciene M , Liu Xiaowei S , Kimmel Donald B , Kulak Jaime , Rabelo Leda M , Zhou Hua , Guo X Edward , Bilezikian John P , Boguszewski Cesar L , Dempster David W 
Published: June 2010

Abstract
Chronic obstructive pulmonary disease (COPD) is associated with osteoporosis and fragility fractures. The objectives of this study were to assess static and dynamic indices of cancellous and cortical bone structure in postmenopausal women with COPD. Twenty women with COPD who had not received chronic oral glucocorticoids underwent bone biopsies after double tetracycline labeling. Biopsies were analyzed by histomorphometry and µCT and compared with age-matched controls. Distribution of the patients according to the Global Initiative for Chronic Obstructive Lung Disease (GOLD) was: Type I (15%), Type II (40%), Type III (30%), and Type IV (15%). Mean (±SD) cancellous bone volume (15.20 ± 5.91 versus 21.34 ± 5.53%, p = .01), trabecular number (1.31  ±  0.26 versus 1.77 ± 0.51/mm, p = .003), and trabecular thickness (141 ± 23 versus 174 ± 36 µm, p = .006) were lower in patients than in controls. Connectivity density was lower in COPD (5.56 ± 2.78 versus 7.94 ± 3.08/mm, p = .04), and correlated negatively with smoking (r = −0.67; p = .0005). Trabecular separation (785 ± 183 versus 614 ± 136 µm, p = .01) and cortical porosity (4.11 ± 1.02 versus 2.32 ± 0.94 voids/mm2; p &lt; .0001) were higher in COPD while cortical width (458 ± 214 versus 762 ± 240 µm; p &lt; .0001) was lower. Dynamic parameters showed significantly lower mineral apposition rate in COPD (0.56 ± 0.16 versus 0.66 ± 0.12 µm/day; p = .01). Patients with more severe disease, GOLD III and IV, presented lower bone formation rate than GOLD I and II (0.028 ± 0.009 versus 0.016 + 0.011 µm3/µm2/day; p = 04). This is the first evaluation of bone microstructure and remodeling in COPD. The skeletal abnormalities seen in cancellous and cortical bone provide an explanation for the high prevalence of vertebral fractures in this disease. © 2010 American Society for Bone and Mineral Research
Nonablative neonatal bone marrow transplantation rapidly reverses severe murine osteopetrosis despite low-level engraftment and lack of selective expansion of the osteoclastic lineage
Authors: Flores Carmen , de Vries Teun J , Moscatelli Ilana , Askmyr Maria , Schoenmaker Ton , Langenbach Geerling EJ , Ehinger Mats , Everts Vincent , Richter Johan 
Published: June 2010

Abstract
Infantile malignant osteopetrosis (IMO) is caused by lack of functional osteoclasts leading to skeletal abnormalities, blindness owing to compression of the optic nerves, bone marrow (BM) failure, and early death. In most patients, TCIRG1, a proton pump subunit essential for bone resorption, is mutated. oc/oc mice represent a model for IMO owing to a deletion in Tcirg1 and die around 4 weeks of age. To determine if hematopoietic stem cell transplantation without prior conditioning can reverse osteopetrosis, neonatal mice were transplanted intravenously with lineage-depleted BM cells. More than 85% of oc/oc mice transplanted with 5 × 106 cells survived long term with an engraftment of 3% to 5% in peripheral blood (PB). At 3 weeks, engraftment in the BM was 1% to 2%, but the cellularity had increased 60-fold compared with untreated oc/oc mice, and RANKL and macrophage colony-stimulating factor (M-CSF) expression in the BM was normalized. Histopathology and micro–computed tomography revealed almost complete reversal of osteopetrosis after 4 weeks. In vitro studies showed that bone resorption by osteoclasts from transplanted oc/oc mice was 14% of transplanted controls, and immunofluorescence microscopy revealed that resorption was mainly associated with osteoclasts of donor origin. Lineage analysis of BM, PB, and spleen did not provide any evidence for selective recruitment of cells to the osteoclastic lineage. The vision also was preserved in transplanted oc/oc mice, as determined by a visual tracking drum test. In summary, nonablative neonatal transplantation leading to engraftment of only a small fraction of normal cells rapidly reverses severe osteopetrosis in the oc/oc mouse model. © 2010 American Society for Bone and Mineral Research.
HNF1α and CDX2 transcriptional factors bind to cadherin‐17 (CDH17) gene promoter and modulate its expression in hepatocellular carcinoma
Authors: Zhu Rui , Wong Kwong‐Fai , Lee Nikki P.Y. , Lee Kai‐Fai , Luk John M.C. 
Published: June 2010

AbstractCadherin‐17 (CDH17) belongs to the cell adhesion cadherin family with a prominent role in tumorigenesis. It is highly expressed in human hepatocellular carcinoma (HCC) and is proposed to be a biomarker and therapeutic molecule for liver malignancy. The present study aims to identify the transcription factors which interact and regulate CDH17 promoter activity that might contribute to the up‐regulation of CDH17 gene in human HCC. A 1‐kb upstream sequence of CDH17 gene was cloned and the promoter activity was studied by luciferase reporter assay. By bioinformatics analysis, deletion and mutation assays, and chromatin immunoprecipitation studies, we identified hepatic nuclear factor 1α (HNF1α) and caudal‐related homeobox 2 (CDX2) binding sites at the proximal promoter region which modulate the CDH17 promoter activities in two HCC cell lines (Hep3B and MHCC97L). A consistent down‐regulation of CDH17 and the two transcriptional activators (HNF1α and CDX2) expression was found in the liver of mouse during development, as well as in human liver cancer cells with less metastatic potential. Suppression of HNF1α and CDX2 expression by small interfering RNA (siRNA) significantly down‐regulated expressions of CDH17 and its downstream target cyclin D1 and the viability of HCC cells in vitro. In summary, we identified the minimal promoter region of CDH17 that is regulated by HNF1α and CDX2 transcriptional factors. The present findings enhance our understanding on the regulatory mechanisms of CDH17 oncogene in HCC, and may shed new insights into targeting CDH17 expression as potential therapeutic intervention for cancer treatment. J. Cell. Biochem. 111: 618–626, 2010. © 2010 Wiley‐Liss, Inc.
Inward rectifier potassium channels in the HL‐1 cardiomyocyte‐derived cell line
Authors: Goldoni Dana , Zhao YouYou , Green Brian D. , McDermott Barbara J. , Collins Anthony 
Published: June 2010

AbstractHL‐1 is a line of immortalized cells of cardiomyocyte origin that are a useful complement to native cardiomyocytes in studies of cardiac gene regulation. Several types of ion channel have been identified in these cells, but not the physiologically important inward rectifier K+ channels. Our aim was to identify and characterize inward rectifier K+ channels in HL‐1 cells. External Ba2+ (100 µM) inhibited 44 ± 0.05% (mean ± s.e.m., n = 11) of inward current in whole‐cell patch‐clamp recordings. The reversal potential of the Ba2+‐sensitive current shifted with external [K+] as expected for K+‐selective channels. The slope conductance of the inward Ba2+‐sensitive current increased with external [K+]. The apparent Kd for Ba2+ was voltage dependent, ranging from 15 µM at −150 mV to 148 µM at −75 mV in 120 mM external K+. This current was insensitive to 10 µM glybenclamide. A component of whole‐cell current was sensitive to 150 µM 4,4′‐diisothiocyanatostilbene‐2,2′‐disulfonic acid (DIDS), although it did not correspond to the Ba2+‐sensitive component. The effect of external 1 mM Cs+ was similar to that of Ba2+. Polymerase chain reaction using HL‐1 cDNA as template and primers specific for the cardiac inward rectifier Kir2.1 produced a fragment of the expected size that was confirmed to be Kir2.1 by DNA sequencing. In conclusion, HL‐1 cells express a current that is characteristic of cardiac inward rectifier K+ channels, and express Kir2.1 mRNA. This cell line may have use as a system for studying inward rectifier gene regulation in a cardiomyocyte phenotype. J. Cell. Physiol. 225: 751–756, 2010. © 2010 Wiley‐Liss, Inc.
The nuclear genes Mtfr1 and Dufd1 regulate mitochondrial dynamic and cellular respiration
Authors: Monticone Massimiliano , Panfoli Isabella , Ravera Silvia , Puglisi Rossella , Jiang Ming‐Ming , Morello Roy , Candiani Simona , Tonachini Laura , Biticchi Roberta , Fabiano Andrea , Cancedda Ranieri , Boitani Carla , Castagnola Patrizio 
Published: June 2010

AbstractDufd1 (DUF729 domain containing 1) is related to Mtfr1 (mitochondrial fission regulator 1), a gene involved in the regulation of antioxidant activity in the mouse testis. The present study was undertaken to better understand their role in regulating mitochondrial architecture and function in the mouse. We show that Dufd1 is expressed as a 2 kb mRNA and has a more specific tissue pattern compared to Mtfr1, with highest level of expression in testes, lower level in spleen, and negligible levels in other organs and/or tissues. In the male gonad, Dufd1 mRNA expression increases during postnatal development, similarly to Mtfr1. In situ hybridization and real‐time PCR analyses show that Dufd1 is expressed in the seminiferous tubules by middle‐late pachytene spermatocytes and spermatids. In transfected cells, the Dufd1‐tagged protein is located in mitochondria, associated with the tips of mitochondrial tubules and to tubules constrictions, and induces mitochondrial fission although with a lesser efficiency than Mtfr1. We also found that both endogenous Dufd1 and Mtfr1 proteins are associated with membrane‐enriched subcellular fractions, including mitochondria. Inhibition of Mtfr1 and/or Dufd1 expression, in a testicular germ cells line, severely impairs O2 consumption and indicates that both genes are required for mitochondrial respiration. Accordingly, analysis of testes mitochondria from Mtfr1‐deficient mice reveals severely reduced O2 consumption and ATP synthesis compared to wt animals. These data show that, in murine testis, Dufd1 and Mtfr1 have redundant functions related to mitochondrial physiology and represent genes with a potential role in testicular function. J. Cell. Physiol. 225: 767–776, 2010. © 2010 Wiley‐Liss, Inc.
The first synthesis of 3‐hydroxy‐2‐(polyfluoroalkyl)chromones and their ammonium salts. 3‐hydroxychromone in the Mannich reaction
Authors: Irgashev Roman A. , Sosnovskikh Vyacheslav Ya. , Sokovnina Anna A. , Röschenthaler Gerd‐Volker 
Published: June 2010

Abstract
magnified image 3‐Hydroxy‐2‐(polyfluoroalkyl)chromones were obtained in good yields via the nitrozation reaction of 2‐(polyfluoroalkyl)chroman‐4‐ones with isopropyl nitrite in the presence of hydrochloric acid. Treatment of 3‐acetoxy‐2‐(trifluoromethyl)chromone with primary amines and hydrazine gave the corresponding ammonium salts. Reaction of 3‐hydroxychromone, prepared by this method, with formaldehyde and α‐aminoacids has been studied. J. Heterocyclic Chem., (2010).
One‐pot synthesis of 2,3‐dihydro‐2‐thioxoquinazolin‐4(1H)‐ones from nitro‐compounds with the aid of tin(II) chloride
Authors: Wang Man‐Man , Dou Guo‐Lan , Shi Da‐Qing 
Published: June 2010

Abstractmagnified imageA facile synthetic method using SnCl2·2H2O system to promote the novel reductive cyclization of 2‐nitrobenzamides and isothiocyanates is described. Sequentially, a series of 2,3‐dihydro‐2‐thioxoquinazolin‐4(1H)‐ones were synthesized in good yields. J. Heterocyclic Chem., (2010).
One‐pot synthesis of new spiro[cyclopropane‐1,3′‐[3H]indol]‐2′(1′H)‐ones from 3‐phenacylideneoxindoles
Authors: Shaabanzadeh Masoud , Khabari Faranak 
Published: June 2010

Abstract
magnified image
In a one‐pot procedure, the 3‐phenacylideneoxindoles 1a, 1b, 1c, 1d were reacted with hydrazine and then in situ with lead(IV) acetate and new diastereoisomers of spiro[cyclopropane‐1,3′‐[3H]indol]‐2′(1′H)‐ones were prepared. Compounds 1a, 1b, 1c, 1d underwent a highly diastereoselective cyclopropanation leading to diastereoisomers 2a, 2b, 2c, 2d. These new compounds containing both 2‐oxindole and cyclopropane moieties may be valuable in medicinal chemistry. J. Heterocyclic Chem., (2010).
Sustaining relationships between communities and local health systems: Two Iranian villages
Authors: Sheikhattari Payam , Burke Jessica G. , O'Keefe Anne Marie , Bazargan‐Hejazi Shahrzad 
Published: June 2010

AbstractThe ability of health systems to positively affect the health and well‐being of communities will be limited if the systems fail to address the crucial role and dynamic nature of communities and ignore key community members and existing institutions. Without a custom for input or an infrastructure for collaboration between individual and institutional community assets and their local health systems, health interventions can lead to failure, duplication or interference with existing health‐related activities. This paper examines the roles of community leaders and institutions in two remote Iranian villages and makes recommendations for improving and sustaining participatory relationships between local communities and their health systems. Copyright © 2010 John Wiley & Sons, Ltd.
Analysis of gene expression changes associated with long‐lasting synaptic enhancement in hippocampal slice cultures after repetitive exposures to glutamate
Authors: Kawaai Katsuhiro , Tominaga‐Yoshino Keiko , Urakubo Tomoyoshi , Taniguchi Naoko , Kondoh Yasumitsu , Tashiro Hideo , Ogura Akihiko , Tashiro Tomoko 
Published: June 2010

AbstractWe have previously shown that repetitive exposures to glutamate (100 μM, 3 min, three times at 24‐hr intervals) induced a long‐lasting synaptic enhancement accompanied by synaptogenesis in rat hippocampal slice cultures, a phenomenon termed RISE (for repetitive LTP‐induced synaptic enhancement). To investigate the molecular mechanisms underlying RISE, we first analyzed the time course of gene expression changes between 4 hr and 12 days after repetitive stimulation using an original oligonucleotide microarray: “synaptoarray.” The results demonstrated that changes in the expression of synapse‐related genes were induced in two time phases, an early phase of 24–96 hr and a late phase of 6–12 days after the third stimulation. Comprehensive screening at 48 hr after the third stimulation using commercially available high‐density microarrays provided candidate genes responsible for RISE. From real‐time PCR analysis of these and related genes, two categories of genes were identified, 1) genes previously reported to be induced by physiological as well as epileptic activity (bdnf, grm5, rgs2, syt4, ania4/carp/dclk) and 2) genes involved in cofilin‐based regulation of actin filament dynamics (ywhaz, ssh1l, pak4, limk1, cfl). In the first category, synaptotagmin 4 showed a third stimulation‐specific up‐regulation also at the protein level. Five genes in the second category were coordinately up‐regulated by the second stimulation, resulting in a decrease in cofilin phosphorylation and an enhancement of actin filament dynamics. In contrast, after the third stimulation, they were differentially regulated to increase cofilin phosphorylation and enhance actin polymerization, which may be a key step leading to the establishment of RISE. © 2010 Wiley‐Liss, Inc.
Composition and antioxidant activity of Thymus vulgaris volatiles: Comparison between supercritical fluid extraction and hydrodistillation
Authors: Grosso Clara , Figueiredo Ana Cristina , Burillo Jesus , Mainar Ana M. , Urieta José S. , Barroso José G. , Coelho José A. , Palavra António M. F. 
Published: June 2010

AbstractSupercritical fluid extraction (SFE) of the volatile oil from Thymus vulgaris L. aerial flowering parts was performed under different conditions of pressure, temperature, mean particle size and CO2 flow rate and the correspondent yield and composition were compared with those of the essential oil isolated by hydrodistillation (HD). Both the oils were analyzed by GC and GC‐MS and 52 components were identified. The main volatile components obtained were p‐cymene (10.0–42.6% for SFE and 28.9–34.8% for HD), γ‐terpinene (0.8–6.9% for SFE and 5.1–7.0% for HD), linalool (2.3–5.3% for SFE and 2.8–3.1% for HD), thymol (19.5–40.8% for SFE and 35.4–41.6% for HD), and carvacrol (1.4–3.1% for SFE and 2.6–3.1% for HD). The main difference was found to be the relative percentage of thymoquinone (not found in the essential oil) and carvacryl methyl ether (1.0–1.2% for HD versus t−0.4 for SFE) which can explain the higher antioxidant activity, assessed by Rancimat test, of the SFE volatiles when compared with HD. Thymoquinone is considered a strong antioxidant compound.
Corrosion behaviour and biocompatibility of a new dental noble AuPdAgTi alloy
Authors: Raducanu D. , Cimpean A. , Vasilescu E. , Cojocaru V. D. , Cinca I. , Drob P. , Ivanescu S. 
Published: June 2010

AbstractThe aims of the study were to investigate the electrochemical response of a new dental Au‐20Pd‐10Ag‐5Ti alloy in artificial Carter‐Brugirard saliva and to assess its biocompatibility using gingival fibroblast culture. Electrochemical techniques of potentiodynamic and linear polarization were used. Also, the open circuit potentials and the corresponding open circuit potential gradients were monitored for 5000 exposure hours. The gingival fibroblast response to Au‐20Pd‐10Ag‐5Ti alloy was estimated by LDH‐cytotoxicity, MTT cell proliferation assays, the assessment of substrate‐dependent changes in extracellular fibronectin network, cell morphology and actin cytoskeleton organization. The cyclic potentiodynamic curves of Au‐20Pd‐10Ag‐5Ti alloy exhibited a passive behaviour with transpassive dissolution of silver, without pitting corrosion. Open circuit potentials were finally stable at noble values. Open circuit potential gradients have low values that could not generate galvanic or local corrosion. The new Au‐20Pd‐10Ag‐5Ti alloy did not promote cell damage and alterations in cell adhesion and morphological features, exhibiting a good biocompatibility.
Developmental and benign movement disorders in childhood
Authors: Bonnet Cecilia , Roubertie Agathe , Doummar Diane , Bahi‐Buisson Nadia , Cochen de Cock Valérie , Roze Emmanuel 
Published: June 2010

AbstractDevelopmental and benign movement disorders are a group of movement disorders with onset in the neonatal period, infancy, or childhood. They are characterized by the absence of associated neurological manifestations and by their favorable outcome, although developmental abnormalities can be occasionally observed. Knowledge of the clinical, neurophysiological, and pathogenetic aspects of these disorders is poor. Based on a comprehensive review of the literature and our practical experience, this article summarizes current knowledge in this area. We pay special attention to the recognition and management of these movement disorders in children. © 2010 Movement Disorder Society
Psychogenic paralysis and recovery after motor cortex transcranial magnetic stimulation
Authors: Chastan Nathalie , Parain Dominique 
Published: June 2010

AbstractPsychogenic paralysis presents a real treatment challenge. Despite psychotherapy, physiotherapy, antidepressants, acupuncture, or hypnosis, the outcome is not always satisfactory with persistent symptoms after long‐term follow‐up. We conducted a retrospective study to assess clinical features and to propose an alternative treatment based on repetitive transcranial magnetic stimulation (rTMS). Seventy patients (44 F/26 M, mean age: 24.7 ± 16.6 years) experienced paraparesis (57%), monoparesis (37%), tetraparesis (3%), or hemiparesis (3%). A precipitating event was observed in 42 patients, primarily as a psychosocial event or a physical injury. An average of 30 stimuli over the motor cortex contralateral to the corresponding paralysis was delivered at low frequency with a circular coil. The rTMS was effective in 89% of cases, with a significantly better outcome for acute rather than chronic symptoms. In conclusion, motor cortex rTMS seem to be very effective in patients with psychogenic paralysis and could be considered a useful therapeutic option. © 2010 Movement Disorder Society