bluuebunny/crossref_metadata_2025_split huggingface dataset:
Production and characterization of anti‐hypertensive and anti‐diabetic peptides from fermented sheep milk with anti‐inflammatory activity: in vitro and molecular docking studies
Authors: Pipaliya Rinkal , Basaiawmoit Bethsheba , Sakure Amar A. , Maurya Ruchika , Bishnoi Mahendra , Kondepudi Kanthi Kiran , Padhi Srichandan , Rai Amit Kumar , Liu Zhenbin , Sarkar Preetam , Hati Subrota 
Published: June 2024

AbstractBACKGROUNDThe present study aimed to evaluate the anti‐hypertensive and anti‐diabetic activities from biologically active peptides produced by fermented sheep milk with Lacticaseibacillus paracasei M11 (MG027695), as well as to purify and characterize the angiotensin‐converting enzyme (ACE) inhibitory and anti‐diabetic peptides produced from fermented sheep milk.RESULTSAfter 48 h of fermentation at 37 °C, sheep milk demonstrated significant changes in anti‐diabetic effects and ACE‐I effects, with inhibition percentages observed for ACE inhibition (76.32%), α‐amylase (70.13%), α‐glucosidase (70.11%) and lipase inhibition (68.22%). The highest level of peptides (9.77 mg mL–1) was produced by optimizing the growth conditions, which included an inoculation rate of 2.5% and a 48 h of incubation period. The comparison of molecular weight distributions among protein fractions was conducted through sodium dodecyl‐sulfate polyacrylamide gel electrophoresis analysis, whereas spots were separated using 2D gel electrophoresis according to both the molecular weight and pH. Peptide characterization with ultra‐filtration membranes at 3 and 10 kDa allowed the study to assess molecular weight‐based separation. Nitric oxide generated by lipopolysaccharide and the secretion of pro‐inflammatory cytokines in RAW 264.7 immune cells were both inhibited by sheep milk fermented with M11. Fourier‐transform infrared spectroscopy was employed to assess changes in functional groups after fermentation, providing insights into the structural changes occurring during fermentation.CONCLUSIONThe present study demonstrates that fermentation with L. paracasei (M11) led to significant changes in fermented sheep milk, enhancing its bioactive properties, notably in terms of ACE inhibition and anti‐diabetic activities, and the generation of peptides with bioactive properties has potential health benefits. © 2024 Society of Chemical Industry.
Optimal pursuit strategies for multi‐pursuer single‐evader games based on cooperative tracking
Authors: Zhou Yinglu , Li Yinya , Sheng Andong , Qi Guoqing , Cong Jinliang 
Published: June 2024

AbstractThis paper investigates a multi‐pursuer single‐evader nonzero‐sum differential game. Unlike traditional treatments, cooperative tracking is considered to design the optimal pursuit strategy by introducing a novel weighted topology and a scalar coupling gain. First, the distributed control strategy is designed based on the Hamilton–Jacobi–Isaacs (HJI) under the nonzero‐sum differential game. Then the modified LQR‐based cooperative tracking strategy is applied to achieve cooperativity among all the pursuers. The interception condition is also analyzed using a coupling gain based on the Laplacian matrix. Several illustrative examples under different scenarios are presented, and the results show that the pursuers with the proposed strategy can successfully intercept the evader.
Consensus recommendations for the use of novel antiretrovirals in persons with HIV who are heavily treatment‐experienced and/or have multidrug‐resistant HIV‐1: Endorsed by the American Academy of HIV Medicine, American College of Clinical Pharmacy
Authors: Cluck David B. , Chastain Daniel B. , Murray Milena , Durham Spencer H. , Chahine Elias B. , Derrick Caroline , Dumond Julie B. , Hester E. Kelly , Jeter Sarah B. , Johnson Melissa D. , Kilcrease Christin , Kufel Wesley D. , Kwong Jeffrey , Ladak Amber F. , Patel Nimish , Pérez Sarah E. , Poe Jonell B. , Bolch Charlotte , Thomas Ian , Asiago‐Reddy Elizabeth , Short William R. 
Published: June 2024

AbstractTreatment options are currently limited for persons with HIV‐1 (PWH) who are heavily treatment‐experienced and/or have multidrug‐resistant HIV‐1. Three agents have been approved by the U.S. Food and Drug Administration (FDA) since 2018, representing a significant advancement for this population: ibalizumab, fostemsavir, and lenacapavir. However, there is a paucity of recommendations endorsed by national and international guidelines describing the optimal use (e.g., selection and monitoring after initiation) of these novel antiretrovirals in this population. To address this gap, a modified Delphi technique was used to develop these consensus recommendations that establish a framework for initiating and managing ibalizumab, fostemsavir, or lenacapavir in PWH who are heavily treatment‐experienced and/or have multidrug‐resistant HIV‐1. In addition, future areas of research are also identified and discussed.
Discovery and biological profile of pyridachlometyl
Authors: Matsuzaki Yuichi , Kurahashi Makoto , Watanabe Satoshi , Kiguchi So , Harada Toshiyuki , Iwahashi Fukumatsu 
Published: June 2024

AbstractPyridachlometyl is a novel tubulin dynamics modulator fungicide developed by Sumitomo as a new agent designed to tackle fungicide resistance. Pyridachlometyl is being developed as a first‐in‐class molecule with an anti‐tubulin mode of action, the chemical structure of which is characterized by a unique tetrasubstituted pyridazine ring. The first commercial product ‘Fuseki flowable’ received initial registration in 2023 in Japan. The concepts of the discovery project, optimization of chemical structures, and biological profiles are reviewed herein. © 2024 The Author(s). Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Use of a fluoroscopy‐based robotic‐assisted total hip arthroplasty system resulted in greater improvements in hip‐specific outcome measures at one‐year compared to a CT‐based robotic‐assisted system
Authors: Ong Christian B. , Buchan Graham B. J. , Hecht Christian J. , Liu David , Petterwood Joshua , Kamath Atul F. 
Published: June 2024

AbstractBackgroundThe purpose of this study was to compare one‐year patient reported outcome measures between a novel fluoroscopy‐based robotic‐assisted (FL‐RTHA) system and an existing computerised tomography‐based robotic assisted (CT‐RTHA) system.MethodsA review of 85 consecutive FL‐RTHA and 125 consecutive CT‐RTHA was conducted. Outcomes included one‐year post‐operative Veterans RAND‐12 (VR‐12) Physical (PCS)/Mental (MCS), Hip Disability and Osteoarthritis Outcome (HOOS) Pain/Physical Function (PS)/Joint replacement, and University of California Los Angeles (UCLA) Activity scores.ResultsThe FL‐RTHA cohort had lower pre‐operative VR‐12 PCS, HOOS Pain, HOOS‐PS, HOOS‐JR, and UCLA Activity scores compared with patients in the CT‐RTHA cohort. The FL‐RTHA cohort reported greater improvements in HOOS‐PS scores (−41.54 vs. −36.55; p = 0.028) than the CT‐RTHA cohort. Both cohorts experienced similar rates of major post‐operative complications, and had similar radiographic outcomes.ConclusionsUse of the fluoroscopy‐based robotic system resulted in greater improvements in HOOS‐PS in one‐year relative to the CT‐based robotic technique.
Analysis of MiR-20b, MIR-197 markers for differentiation between forensic body fluids encountered in sexual assault cases
Authors: Alsaeed Shimaa Ahmed , Elrewieny Noha Maher , Eltokhy Rabab Abdulmoez Amin , Mohamed Mohamed Shokr , Khalil Wagdy K. B. , Shalby Aziza B. , Booles Hoda F. , Aboubakr Heba Mohamed 
Published: June 2024

AbstractIdentifying body fluids can be a critical clue that aids in reconstructing the crime scene. Semen and vaginal fluid identification is crucial, especially in cases of sexual assault. The majority of forensic studies focused on identifying normal body fluids and neglected the expression variation of semen in pathology. To differentiate between vaginal fluids, fertile and infertile semen samples (oligospermia and azoospermia) using miR 20b and miR197. A total of 48 body fluid samples, divided as 16 vaginal fluids, 16 fertile semen, and 16 infertile semen samples (8 with oligospermia and 8 with azoospermia), were collected, and the expression levels of miR-20b and miR-197 were detected by the SYBR Green real-time quantitative PCR technique. Our results showed significant different expression of these miRNAs in normal semen compared to vaginal and infertile semen. Moreover, we designed a model based on Fisher’s discriminant function to forecast the group affiliations of unidentified samples. With three novel equations, we were able to accurately distinguish between semen and vaginal fluid, fertile and infertile semen, and oligospermia and azoospermia semen samples with validation accuracy of 81.3%, 100%, and 100%, respectively. MiR-20b and miR-197 expression levels are efficient and appropriate markers to distinguish semen from vaginal fluid and to differentiate between fertile and infertile semen samples. However, the present study is a preliminary study based on clinical samples, and the potential role of these markers in differentiating real crime scene samples is still unknown, so we recommend further research to investigate these markers expression while using forensic samples.
CD4 nadir and neurocognitive trajectories in people living with HIV
Authors: Garabet Razmig , Dampier Will , Tillman Shinika , Malone Kim , Szep Zsofia , Althoff Amy , Pirrone Vanessa , Nonnemacher Michael R. , Wigdahl Brian , Schultheis Maria , Devlin Kathryn N. 
Published: June 2024

AbstractHuman immunodeficiency virus-associated neurocognitive disorders persist in the combination antiretroviral therapy era. CD4 nadir is a well-established predictor of cognition cross-sectionally, but its impact on longitudinal neurocognitive (NC) trajectories is unclear. The few studies on this topic examined trajectories of global cognition, rather than specific NC domains. The current study examined CD4 nadir in relation to domain-specific NC decline. 132 HIV + adults from the Temple/Drexel Comprehensive NeuroHIV Center, Clinical and Translational Research Support Core Cohort were administered comprehensive NC assessments longitudinally, with last visit occurring an average of 12 years after CD4 nadir. Linear mixed models were used to examine CD4 nadir in relation to longitudinal NC trajectories in three empirically identified NC domains: speed/executive function (S/EF), visuospatial memory (VM), and verbal fluency (VF). CD4 nadir was associated with change in VF (p = 0.020), but not with S/EF or VM. Specifically, those with CD4 nadir < 200 demonstrated increasing VF over time (p = .002), whereas those with CD4 nadir > 200 demonstrated stable VF (p = .568), though these differing trajectories may partly reflect regression to the mean or differential practice effect. CD4 dynamics over time were analyzed as potential mechanisms for the identified associations, with mixed findings. While low CD4 nadir has been associated with weaker neurocognition among people living with HIV, the results of this study suggest that low CD4 nadir is not associated with ongoing decline a decade later. Nadir-related deficits in VF may be stable or even improve over time, possibly reflecting the beneficial cognitive effects of long-term treatment and immune reconstitution.
Study on the dynamic constitutive model of coal at different depths in Pingdingshan mining area
Authors: Hao Haichun , Gao Mingzhong , Li Shengwei , Li Yexue , Zeng Gang 
Published: June 2024

AbstractThe environment of deep in-situ occurrence is complex, and secondary dynamic disasters occur frequently. Exploring the dynamic constitutive models of coal at different depths is the foundation for deep resource development, which can help promote the safe and efficient mining of coal resources at different depths and reveal the mechanism of sudden dynamic disasters. Dynamic mechanical testing of coal at different depths of the Pingdingshan mine (China) was carried out using a split Hopkinson pressure bar system with confining pressure. On the basis of statistical damage theory, dynamic constitutive models of coal at different depths were explored, and the theoretical models were compared and verified using the experimental results. An expression relating to the infinitesimal element strength of coal and loading rate was derived, and the parameters σ1dp, Ed, and ε1dp were determined. Inversion verification of the model showed that the experimental results agree well with the theoretical results prior to the peak stress. These research results are expected to be applied to the exploitation of deep resources, laying a foundation for the law of deep earth science and the construction of deep engineering.
Research on the early warning indicator system for public safety risks in county areas of the Yangtze River Delta region during urbanization process
Authors: Ke Yin 
Published: June 2024

AbstractChina’s intense urbanization has profoundly impacted county-level societies, leading to increased diversity and contradictions, and pushing these communities towards a risk society. This is especially pronounced in the Yangtze River Delta, a region of peak urbanization. The stability and progress of these local societies are vital for the region’s overall high-quality development. Yet, with ongoing urbanization, these areas face numerous challenges. To effectively tackle these issues, a robust system encompassing pre-evaluation, early warning, and emergency preparedness is essential. This study explores the relationships and structures among various risk factors using grounded theory analysis. Furthermore, it applies the Analytic Hierarchy Process (AHP) to develop a comprehensive risk early warning index system, significantly enhancing the response capabilities to public security challenges in the Yangtze River Delta’s county-level societies.
EU’s Medical Device Expert Panels: Analysis of Membership and Published Clinical Evaluation Consultation Procedure (CECP) Results
Authors: Watson Colleen , Richmond Frances J. 
Published: June 2024

Abstract
Background
The new EU Medical Device Regulation (MDR) places greater importance on the role of clinical evidence to establish safety and performance. Article 54 of the MDR calls for expert committees to independently review the scientific, technical, and clinical evidence supporting the market authorization of certain novel devices independently from the established process of Notified Body reviews. These experts provide a review and opinion that ultimately is taken into consideration alongside the information reviewed by the Notified Body during the review process. Four expert committees (General and Plastic Surgery and Dentistry; Orthopaedics, Traumatology, Rehabilitation, Rheumatology; Circulatory System; and Neurology) have published at least one Scientific Opinion (SO) under the Clinical Evaluation Consultation Procedure (CECP) in 2021–2022.

Methods
The four expert committees with published CECP opinions were reviewed to assess the academic backgrounds and professional expertise of each member with respect to clinical, technical, and biological domains on a 0–2 scale for each domain. A content review was conducted on the 10 CECP opinions published by these committees to assess their consistency with the goals and outcome expectations set by the MDR. The extent of content related to each of the clinical, technical, and biological domains was also assessed on a 0–2 scale.

Results
All committees were composed primarily by members with strong clinical expertise, but only a few had strong technical and biological expertise. Across committees, the average scores of members related to academic background and professional expertise both ranged from 1.64 to 2.00 in the clinical domain, but only 0–0.15 and 0.15–0.69, respectively, in the biological domain, and 0.12–0.55 and 0.23–0.73, respectively, in the technical domain. A content review for the 10 SOs showed that all opinions focused exclusively or primarily on the clinical evidence. Three contained a modest amount of additional text directed at technical/engineering issues and five at biological issues.

Conclusion
Expert committees are composed predominantly of expert clinical reviewers but have many fewer members with significant technical or biological expertise. This may limit the ability of the committees to evaluate the significant technical and biological risks that are often best understood by preclinical testing. Broadening the expertise across the committees may improve the depth of their benefit/risk critiques.
Cultural differences in teacher immediacy and classroom communication apprehension: an empirical study between Brazil and the United States
Authors: Parente Alves Neta Zeneida 
Published: June 2024

AbstractTeacher immediacy and classroom communication apprehension have been well researched in the United States. Instructional communication research in Brazil is limited, and several topics still demand investigation among the population of Brazil. This study extends current research on teacher immediacy and classroom communication apprehension to the Brazilian population. We should extend the analysis to the Brazilian context instead of relying on previous investigations from other regions as the basis for cross-cultural theorizing. A total of 221 students participated in the survey. This study reports four significant findings: (1) Brazilian students’ perceptions of teacher immediacy are greater than American students’ perceptions of teacher immediacy; (2) American students have higher levels of Classroom Communication Apprehension than Brazilian students; (3) Classroom communication apprehension is negatively correlated with American students’ perceptions of teacher immediacy; and (4) Classroom communication apprehension is not significantly correlated with Brazilian students’ perceptions of teacher immediacy. Highly immediate teachers will have less apprehensive students.
Predicting sales and cross-border e-commerce supply chain management using artificial neural networks and the Capuchin search algorithm
Authors: Xie Lun , Liu Jiaquan , Wang Wei 
Published: June 2024

AbstractE-commerce provides a large selection of goods for sale and purchase, which promotes regular transactions and commodity flows. Efficient distribution of goods and precise estimation of customer wants are essential for cost reduction. In order to improve supply chain efficiency in the context of cross-border e-commerce, this article combines machine learning approaches with the Internet of Things. The suggested approach consists of two main stages. Order prediction is done in the first step to determine how many orders each merchant is expected to get in the future. In the second phase, allocation operations are conducted and resources required for each retailer are supplied depending on their needs and inventory, taking into account each store’s inventory as well as the anticipated sales level. This suggested approach makes use of a weighted mixture of neural networks to anticipate sales orders. The Capuchin Search Algorithm (CapSA) is used in this weighted combination to concurrently enhance the learning and ensemble performance of models. This indicates that an effort is made to reduce the local error of the learning model at the model level via model weight adjustments and neural network configuration. To guarantee more accurate output from the ensemble model, the best weight for each individual component is found at the ensemble model level using the CapSA method. This method yields the ensemble model’s final output in the form of weighted averages by choosing suitable weight values. With a Root Mean Squared Error of 2.27, the suggested technique has successfully predicted sales based on the acquired findings, showing a minimum decrease of 2.4 in comparison to the comparing methodologies. Additionally, the suggested method’s strong performance is shown by the fact that it was able to minimize the Mean Absolute Percentage Error by 14.67 when compared to other comparison approaches.
Solubility of lumiracoxib in supercritical carbon dioxide
Authors: Albadran Firas H. , Abbood Nabeel K. , Al-Mayyahi Mohammad A. , Hosseini Seyednooroldin , Abed Mohammed S. 
Published: June 2024

AbstractThis study aims to use a static-based solubility method for measuring the solubility of lumiracoxib at a temperature of 308–338 K and pressure of 120–400 bar for the first time. The obtained solubility data for lumiracoxib is between 4.74 × 10−5 and 3.46 × 10−4 (mole fraction) for the studied ranges of pressure and temperature. The solubility values reveal that the lumiracoxib experiences a crossover pressure of about 160 bar. Moreover, the measured solubility data of these two drugs are correlated with density-based semi-empirical correlations namely Bartle et al., Mendez-Santiago-Teja, Kumar and Johnstone, Chrastil and modified Chrastil models with an average absolute relative deviation of 10.7%, 9.5%, 9.8%, 7.8%, and 8.7% respectively for lumiracoxib. According to these findings, it is obvious that all of the examined models are rather accurate and there is no superiority between these models for both examined drugs although the Chrastil model is slightly better in the overall view.
Stress-related transcriptomic changes associated with GFP transgene expression and active transgene silencing in plants
Authors: Kallemi Paraskevi , Verret Frederic , Andronis Christos , Ioannidis Nikolaos , Glampedakis Nikolaos , Kotzabasis Kiriakos , Kalantidis Kriton 
Published: June 2024

AbstractPlants respond to biotic and abiotic stress by activating and interacting with multiple defense pathways, allowing for an efficient global defense response. RNA silencing is a conserved mechanism of regulation of gene expression directed by small RNAs important in acquired plant immunity and especially virus and transgene repression. Several RNA silencing pathways in plants are crucial to control developmental processes and provide protection against abiotic and biotic stresses as well as invasive nucleic acids such as viruses and transposable elements. Various notable studies have shed light on the genes, small RNAs, and mechanisms involved in plant RNA silencing. However, published research on the potential interactions between RNA silencing and other plant stress responses is limited. In the present study, we tested the hypothesis that spreading and maintenance of systemic post-transcriptional gene silencing (PTGS) of a GFP transgene are associated with transcriptional changes that pertain to non-RNA silencing-based stress responses. To this end, we analyzed the structure and function of the photosynthetic apparatus and conducted whole transcriptome analysis in a transgenic line of Nicotiana benthamiana that spontaneously initiates transgene silencing, at different stages of systemic GFP-PTGS. In vivo analysis of chlorophyll a fluorescence yield and expression levels of key photosynthetic genes indicates that photosynthetic activity remains unaffected by systemic GFP-PTGS. However, transcriptomic analysis reveals that spreading and maintenance of GFP-PTGS are associated with transcriptional reprogramming of genes that are involved in abiotic stress responses and pattern- or effector-triggered immunity-based stress responses. These findings suggest that systemic PTGS may affect non-RNA-silencing-based defense pathways in N. benthamiana, providing new insights into the complex interplay between different plant stress responses.