bluuebunny/crossref_metadata_2025_split huggingface dataset:
Penetration depth method—novel real‐time strategy for evaluating femoroacetabular impingement
Authors: Arbabi Ehsan , Chegini Salman , Boulic Ronan , Tannast Moritz , Ferguson Stephen J. , Thalmann Daniel 
Published: January 2010

AbstractWe introduce a new method for computerized real‐time evaluation of femoroacetabular impingement (FAI). In contrast to previously presented stress analyses, this method is based on two types of predictions of penetration depths for two rotating bodies: curvilinear and radial penetration depth. This intuitive method allows the analysis of both bony and soft tissue structures (such as cartilage and acetabular labrum) in real time. Characteristic penetration depth patterns were found for different subtypes of FAI, such as cam and pincer pathologies. In addition, correlation between the penetration depths (estimated by applying this method) and the existing contact stresses (estimated by applying the finite element method) of various hip morphologies were found. A strong correlation with predicted stress values existed, with a mean correlation coefficient of 0.91 for the curvilinear and 0.80 for the radial penetration method. The results show that the penetration depth method is a promising, fast, and accurate method for quantification and diagnosis of FAI. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:880–886, 2010
Changes in knee adduction moment, pain, and functionality with a variable‐stiffness walking shoe after 6 months
Authors: Erhart Jennifer C. , Mündermann Annegret , Elspas Barbara , Giori Nicholas J. , Andriacchi Thomas P. 
Published: January 2010

AbstractThis study tested the effects of variable‐stiffness shoes on knee adduction moment, pain, and function in subjects with symptoms of medial compartment knee osteoarthritis over 6 months. Patients were randomly and blindly assigned to a variable‐stiffness intervention or constant‐stiffness control shoe. The Western Ontario and McMaster Universities (WOMAC) score served as the primary outcome measure. Joint loading, the secondary outcome measure, was assessed using the external knee adduction moment. Peak external knee adduction moment, total WOMAC, and WOMAC pain scores were assessed at baseline and after 6 months. The total WOMAC and WOMAC pain scores for the intervention group were reduced from baseline to 6 months (p = 0.017 and p = 0.002, respectively), with no significant reductions for the control group. There was no difference between groups in magnitude of the reduction in total WOMAC (p = 0.50) or WOMAC pain scores (p = 0.31). The proportion of patients achieving a clinically important improvement in pain was greater in the intervention group than in the control group (p = 0.012). The variable‐stiffness shoes reduced the peak knee adduction moment (−6.6% vs. control, p < 0.001) in the 34 intervention subjects at 6 months. The adduction moment reduction significantly improved (p = 0.03) from the baseline reduction. The constant‐stiffness control shoe increased the peak knee adduction moment (+6.3% vs. personal, p = 0.004) in the 26 control subjects at 6 months. The results of this study showed that wearing the variable‐stiffness shoe lowered the adduction moment, reduced pain, and improved functionality after 6 months of wear. The lower adduction moment associated with wearing this shoe may slow the rate of progression of osteoarthritis after long‐term use. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:873–879, 2010
Scaffold‐free tissue‐engineered cartilage implants for laryngotracheal reconstruction
Authors: Gilpin David A. , Weidenbecher Mark S. , Dennis James E. 
Published: January 2010

AbstractObjectives/Hypothesis:Donor site morbidity, including pneumothorax, can be a considerable problem when harvesting cartilage grafts for laryngotracheal reconstruction (LTR). Tissue engineered cartilage may offer a solution to this problem. This study investigated the feasibility of using autologous chondrocytes to tissue‐engineer scaffold‐free cartilage grafts for LTR in rabbits to avoid degradation that often arises from an inflammatory reaction to scaffold carrier matrix.Study Design:Animal study.Methods:Auricular cartilage was harvested from seven New Zealand white rabbits, the chondrocytes expanded and loaded onto a custom‐made bioreactor for 7 to 8 weeks to fabricate autologous scaffold‐free cartilage sheets. The sheets were cut to size and used for LTR, and the rabbits were sacrificed 4, 8, and 12 weeks after the LTR and prepared for histology.Results:None of the seven rabbits showed signs of respiratory distress. A smooth, noninflammatory scar was visible intraluminally; the remainder of the tracheal lumen was unremarkable. Histologically, the grafts showed no signs of degradation or inflammatory reaction, were covered with mucosal epithelium, but did show signs of mechanical failure at the implantation site.Conclusions:These results show that autologous chondrocytes can be used to fabricate an implantable sheet of cartilage that retains a cartilage phenotype, becomes integrated, and does not produce a significant inflammatory reaction. These findings suggest that with the design of stronger implants, these implants can be successfully used as a graft for LTR. Laryngoscope, 2010
Ambulatory sinus and nasal surgery in the United States: Demographics and perioperative outcomes
Authors: Bhattacharyya Neil 
Published: January 2010

AbstractObjectives/Hypothesis:Determine the prevalence of ambulatory sinonasal surgical procedures and perioperative outcomes in the United States.Methods:The National Survey of Ambulatory Surgery 2006 release was examined, extracting all cases of nasal or sinus surgery. Sinonasal surgical procedures were grouped according to septoplasty and/or turbinate surgery, sinus surgery, facial plastic surgery, and other procedures. Overall and group population‐based sinonasal procedure prevalences were estimated from the sample. Frequency of general anesthesia, discharge status, and unexpected emergency room visit estimates were obtained. Perioperative complication rates for cardiac arrest, malignant hyperthermia, postoperative emesis, and blood transfusion were determined as well.Results:Overall, an estimated 600,000 patients underwent ambulatory sinonasal procedures in 2006 with a mean age of 40.4 years and 53.7% male predominance. On a per patient basis, there were 260,000 septoplasties, 257,000 sinus cases, 134,000 plastics cases, and 103,000 other cases. Facial plastics procedures were less likely to use general anesthesia (72.8%) than the overall cohort (90.7%, P < .001). The vast majority of patients were discharged to home with an unexpected admission rate of 2.65%. Among the sample, there were no cases of cardiac arrest, malignant hyperthermia, or blood transfusion. The postprocedure emesis rate was 1.3%, not significantly different among procedure groups (P = .692). Only 0.1% of patients visited the emergency room postprocedure.Conclusions:Approximately 600,000 ambulatory sinonasal cases are performed each year in the United States. Complication rates and unexpected postprocedure mission rates are quite low indicating the safety and effectiveness of the ambulatory setting for sinonasal surgery. Laryngoscope, 2010
Parotidotomy approach for a midcheek mass: A new surgical strategy
Authors: Jung Young Ho , Hah J. Hun , Sung Myung‐Whun , Kim Kwang Hyun 
Published: January 2010

AbstractObjectives/Hypothesis:To report the feasibility and the results of a new surgical strategy for midcheek masses we called the parotidotomy approach.Study Design:Retrospective consecutive case series.Methods:Characteristics, surgical outcome, and cosmetic result of cases managed using the parotidotomy approach were collected by retrospectively reviewing medical records. The standard superficial parotidectomy approach was modified to dissect facial nerve branches selectively to obtain a symmetric facial contour. The parotid gland was fully bisected along the course of the zygomatic and buccal branches of the facial nerve to provide access to the midcheek mass. The bisected parotid gland was repositioned after mass excision.Results:Seven patients (3 males and 4 females) were included in this study. The parotidotomy approach was accomplished in two cases with a malignant tumor (one acinic cell carcinoma, one low‐grade mucoepidermoid carcinoma), four with a benign tumor (two pleomorphic adenoma, one basal cell adenoma, one facial nerve schwannoma), and in one case with a chronic inflammatory lesion (chronic sialadenitis). In no case was facial nerve paralysis or Frey's syndrome noticed after this approach. For the two malignant tumors, there was no evidence of recurrence or metastasis at 2‐year and 2.5‐year follow‐ups. Six of the seven patients were fully satisfied with the cosmetic results of surgery. The remaining patient had a mild sunken deformity of the midcheek where the mass had been located.Conclusions:To manage midcheek masses we modified the standard parotidectomy approach. This new surgical strategy, which we named the parotidotomy approach, provides surgically safe and cosmetically excellent results for midcheek masses. Laryngoscope, 2010
Blends of Oppositely Charged PEG–PPG–PEG Copolymers Displaying Improved Physical Thermogelling Properties
Authors: Lee Seung Yong , Lee Yuhan , Chae Su Young , Park Tae Gwan , Ahn Cheol‐Hee 
Published: January 2010

AbstractOppositely charged F127 copolymers with a substitution ratio >95% were synthesized by end‐group modification. Single succinic anhydride end‐capped F127 (F127‐SA) or triple (F127‐Tris‐SA) carboxylic acid groups were introduced to the distal ends via esterification. F127 was also end‐capped with two types of amines, triethylenetetramine (F127‐TETA) and ethylene diamine (F127‐EDA). Oppositely charged copolymers were solution‐blended to produce co‐micellized nanostructures at the optimized blend compositions, as determined by ξ‐potential measurement and the composition dependence of the sol–gel phase‐transition temperature. F127 exhibited the approximate storage modulus of 40 000 Pa, while the 1:1 w/w F127‐SA/F127‐TETA and 1:3 w/w F127‐Tris‐SA/F127‐EDA blend displayed a modulus of 90 000 and 170 000 Pa, respectively. Hydrogels from negatively charged F127‐SA or positively charged F127‐TETA homopolymer showed the lower storage modulus. The rheological properties of the thermogelling polymers were enhanced by simple modification of the distal ends of F127 copolymers as well as by the additional ionic interactions between oppositely charged micelles.magnified image
Swelling and Viscoelastic Characterisation of pH‐Responsive Chitosan Hydrogels for Targeted Drug Delivery
Authors: Jahren Susannah L. , Butler Michael F. , Adams Sarah , Cameron Ruth E. 
Published: January 2010

AbstractChitosan hydrogels have been investigated as potential vehicles for targeted drug delivery. The gels were permanently crosslinked with conventional crosslinking agent, glutaraldehyde and the naturally occurring non‐toxic crosslinking agent genipin in both the monomer and polymerised states. The swelling behaviour of all the gels was highly pH dependent. The gels with the lowest crosslink density not only had an increased rate of diffusion but also an increased total release. It is shown that by altering the type and degree of crosslinking in a chitosan hydrogel it is possible to change the swelling and elastic characteristics of the material, which could be of potential interest as an environment responsive drug delivery matrix.magnified image
EPR/Spin‐trapping study of free radical intermediates in the photolysis of trifluoromethyl ketones with initiators
Authors: Rosa Esmeralda , Guerrero Angel , Bosch Mª Pilar , Julià Luis 
Published: January 2010

AbstractPhotolysis of trifluoromethyl ketones (TFMKs) 1a–1e versus the non‐fluorinated ketones 2a–2b in the presence of radical initiators by electron paramagnetic resonance spectroscopy has been studied for the first time. The transient radicals generated after irradiation of the ketones were identified by trapping with 2‐methyl‐2‐nitrosopropane (MNP) and 2,4,6‐tri‐tert‐butylnitrosobenzene (TTBNB) as spin traps. TTBNB is a powerful, particularly useful spin trap in these kinds of processes producing anilino and nitroxyl spin adducts due to the ambivalent reactivity on the N and O atoms. In the presence of t‐butylperoxide, short‐chain TFMKs, such as 1,1,1‐trifluoroacetone (1d) and hexafluoroacetone (1e), give rise to detection of the elusive trifluoromethyl radical. In contrast, long‐chain TFMKs did not provide clues to prove formation of the trifluoromethyl radical but instead to radicals derived by abstraction of one α‐methylene proton to the carbonyl. Although TFMKs are quite stable to photodegradation in the absence of initiator, methyl ketone 2b and phenyl ketone 3 produce radicals resulting from abstraction of a γ‐hydrogen to the carbonyl group. Copyright © 2010 John Wiley & Sons, Ltd.
jTraqX: A free, platform independent tool for isobaric tag quantitation at the protein level
Authors: Muth Thilo , Keller Daniela , Puetz Stephanie Michaela , Martens Lennart , Sickmann Albert , Boehm Andreas M. 
Published: January 2010

AbstractMany proteomic studies focus on quantitative aspects, using different stable isotope labeling techniques that require specialized software to analyze the generated data. Here we present jTraqX, an easy‐to‐use tool for processing and visualizing protein quantification data. jTraqX is platform independent and is compatible with all available 4‐plex isobaric tags. jTraqX can be freely downloaded at http://sourceforge.net/projects/protms.
MS‐driven protease substrate degradomics
Authors: Impens Francis , Colaert Niklaas , Helsens Kenny , Plasman Kim , Van Damme Petra , Vandekerckhove Joël , Gevaert Kris 
Published: January 2010

AbstractProteolytic processing has recently received increased attention in the field of signal propagation and cellular differentiation. Because of its irreversible nature, protein cleavage has been associated with committed steps in cell function. One aspect of protease biology that boomed the past few years is the detailed characterization of protease substrates by both shotgun as well as targeted MS‐driven proteomics techniques. The most promising techniques are discussed in this review and we further elaborate on the bioinformatics challenges that accompany mainly qualitative, MS‐driven protease substrate degradome studies.
Defining topological features of membrane proteins by nanoelectrospray ionisation mass spectrometry
Authors: Jones Lynsey N. , Baldwin Stephen A. , Henderson Peter J. F. , Ashcroft Alison E. 
Published: January 2010

AbstractThe D‐galactose‐H+symport protein, GalP, ofEscherichia coliis the bacterial homologue of the human glucose transport protein, GLUT1. Here we demonstrate that mass spectrometry can be used to map modification by covalently bound reagents, and also to detect structural changes in the GalP protein that occur upon substrate binding. The small thiol‐group‐specific reagentN‐ethylmaleimide (NEM) was used to modify the cysteine residues in GalP(His)6both alone and in the presence of D‐glucose, a known substrate. Employing a mixture of proteolysis and thermal degradation methods, the three cysteine residues were found to undergo sequential reactions with NEM, with Cys374 being modified first, followed by Cys389 and finally Cys19, thus indicating their different accessibilities within the three‐dimensional structure of the protein. Prior binding of the substrate D‐glucose to the protein protected Cys19 and Cys374 against NEM modification, but not Cys389. Cys374 had been expected to be shielded by D‐glucose binding while Cys389 had been expected to be unaffected, consistent with their proposed respective locations in the vicinity of, and distant from, the sugar binding site. However, the inaccessibility of Cys19 was unexpected and suggests a structural change in the protein promoted by D‐glucose binding which changes the proximity of Cys19 with respect to the D‐glucose‐binding site. Copyright © 2010 John Wiley & Sons, Ltd.
Glycogen storage disease type IV: novel mutations and molecular characterization of a heterogeneous disorder
Authors: Li Sing‐Chung , Chen Chiao‐Ming , Goldstein Jennifer L. , Wu Jer‐Yuarn , Lemyre Emmanuelle , Burrow Thomas Andrew , Kang Peter B. , Chen Yuan‐Tsong , Bali Deeksha S. 
Published: January 2010

AbstractGlycogen storage disease type IV (GSD IV; Andersen disease) is caused by a deficiency of glycogen branching enzyme (GBE), leading to excessive deposition of structurally abnormal, amylopectin‐like glycogen in affected tissues. The accumulated glycogen lacks multiple branch points and thus has longer outer branches and poor solubility, causing irreversible tissue and organ damage. Although classic GSD IV presents with early onset of hepatosplenomegaly with progressive liver cirrhosis, GSD IV exhibits extensive clinical heterogeneity with respect to age at onset and variability in pattern and extent of organ and tissue involvement. With the advent of cloning and determination of the genomic structure of the human GBE gene (GBE1), molecular analysis and characterization of underlying disease‐causing mutations is now possible. A variety of disease‐causing mutations have been identified in the GBE1 gene in GSD IV patients, many of whom presented with diverse clinical phenotypes. Detailed biochemical and genetic analyses of three unrelated patients suspected to have GSD IV are presented here. Two novel missense mutations (p.Met495Thr and p.Pro552Leu) and a novel 1‐bp deletion mutation (c.1999delA) were identified. A variety of mutations in GBE1 have been previously reported, including missense and nonsense mutations, nucleotide deletions and insertions, and donor and acceptor splice‐site mutations. Mutation analysis is useful in confirming the diagnosis of GSD IV—especially when higher residual GBE enzyme activity levels are seen and enzyme analysis is not definitive—and allows for further determination of potential genotype/phenotype correlations in this disease.
Surgical synovectomy of the knee in young haemophiliacs: Long-term results of a monocentric series of 23 patients
Authors: Rampal Virginie , Odent T. , Torchet M. F. , Rothschild C. , Elie C. , Glorion C. , Padovani J. P. 
Published: January 2010

Background In haemophiliacs, synovectomy is indicated for recurrent joint bleedings, despite medical treatment. Method We report a series of 23 surgical synovectomies of the knee with a median follow-up of 8.8 years. The median age of patients at surgery was 13.5 years. Clinical and radiological evaluations were made according to the Petrini and the Pettersson scores, at 1 and 5 years after surgery, and at the last follow-up. Wilcoxon and Spearman's tests were used for the statistical analysis. Result The Petrini score improved at 1 and 5 years ( P < 0.001). Nine patients have 20 years of follow-up and a stable result. In more than half of the knees, no episode of recurrent bleeding occurred. The effect of surgery on the range of motion (ROM) was moderate and mobilisation under anaesthesia did not improve it significantly. There was a progressive worsening of the radiological score, but no correlation between clinical and radiological score was noticed ( ρ = 0.08, P = 0.77). Conclusion Complete synovectomy gives good long-term results in term of bleeding recurrence and overall function.
Point-of-care ultrasound diagnosis of SCFE
Authors: Stone Michael B. , Barrett Chameeka 
Published: January 2010

Abstract

Introduction
A 13-year-old boy presented to the emergency department with left knee pain after a fall. Initial radiographs were unremarkable, but the child returned to the emergency department 6 weeks later with persistent symptoms.

Materials and methods
A bedside sonogram of the left hip performed by the treating emergency physician demonstrated a widened epiphyseal plate and an associated effusion, consisted with a slipped capital femoral epiphysis (SCFE). Repeat radiographs confirmed the diagnosis.

Conclusion
The ultrasound features and test characteristics for the detection of SCFE are reviewed.
Sublingual misoprostol for first trimester termination of pregnancy
Authors: Fekih Myriam , Fathallah Khadija , Regaya Lassad Ben , Bouguizane Sassi , Chaieb Anouar , Bibi Mohamed , Khairi Hedi 
Published: January 2010

AbstractObjectivesTo compare blood loss, efficiency, and acceptability of repeated doses of sublingual misoprostol with the standard Tunisian regimen of mifepristone–misoprostol for first trimester medical abortion.MethodsA prospective randomized trial of 252 healthy pregnant women requesting medical abortion in the first trimester (up to 56 days). Participants were randomized to receive 200 mg of oral mifepristone followed by 400 µg of oral misoprostol (group 1) or 800 µg of sublingual misoprostol repeated every 4 hours for up to a maximum of 3 doses (group 2). Primary outcome was blood loss assessed by decrease in hematocrit.ResultsMean decrease in hematocrit at follow‐up was significantly greater in group 1 than in group 2 (3.65% ± 1.18% vs 2.69% ± 1.89%, respectively; P = 0.02). There was no difference in efficiency rates between groups 1 and 2 (94.5% vs 92.1%; P = 0.7). Comparable proportions of women experiencing at least one adverse effect after misoprostol administration were reported in groups 1 and 2 (81.7% vs 79.4%; P = 0.75).ConclusionCompared with the most widely used regimen in Tunisia (mifepristone–misoprostol), sublingual misoprostol alone induces less blood loss (although not clinically significant); it is less expensive and offers reduced interval time to expulsion.
Multi-access antenna for an opportunistic radio mobile communication of fourth generation
Authors: El Hajj Walid , Gallée François , Person Christian 
Published: January 2010

A new model of two-access reconfigurable antennas for future mobile communication systems is presented in this article. This structure is based on a slot antenna with two separated access ports, isolated and matched at 1 and 2 GHz, respectively. The novelty of this element lies in the fact that first a filtering structure is integrated in the antenna, and then any additional switching or frequency path selecting components that would induce losses and noise degradation is suppressed. Such flexible structures are assumed to be used in a future opportunistic radio, incorporating special features of “spectrum sensing”. The concept of the antenna illustrated by simulation and measurement is presented.
Activity profiles for marine sponge-associated bacteria obtained by 16S rRNA vs 16S rRNA gene comparisons
Authors: Kamke Janine , Taylor Michael W , Schmitt Susanne 
Published: January 2010

Abstract
The phylogenetic diversity of microorganisms in marine sponges is becoming increasingly well described, yet relatively little is known about the activities of these symbionts. Given the seemingly favourable environment provided to microbes by their sponge hosts, as indicated by the extraordinarily high abundance of sponge symbionts, we hypothesized that the majority of sponge-associated bacteria are active in situ. To test this hypothesis we compared, for the first time in sponges, 16S rRNA gene- vs 16S rRNA-derived bacterial community profiles to gain insights into symbiont composition and activity, respectively. Clone libraries revealed a highly diverse bacterial community in Ancorina alata, and a much lower diversity in Polymastia sp., which were identified by electron microscopy as a high- and a low-microbial abundance sponge, respectively. Substantial overlap between DNA and RNA libraries was evident at both phylum and phylotype levels, indicating in situ activity for a large fraction of sponge-associated bacteria. This active fraction included uncultivated, sponge-specific lineages within, for example, Actinobacteria, Chloroflexi and Gemmatimonadetes. This study shows the potential of RNA vs DNA comparisons based on the 16S rRNA gene to provide insights into the activity of sponge-associated microorganisms.