bluuebunny/crossref_metadata_2025_split huggingface dataset:
Real-world insights and outcomes related to ciclosporin A 0.1% cationic emulsion for the long-term treatment of dry eye disease in Germany: Country-level sub-analysis of the PERSPECTIVE study
Authors: Lanzl Ines , Deuter Christoph M E , Lorenz Katrin , Geerling Gerd ,   on behalf of the PERSPECTIVE German study group
Published: May 2024

Abstract
Purpose
The PERSPECTIVE study was a real-world European, non-interventional, multicenter, observational study that evaluated the effectiveness, tolerability, and safety of ciclosporin A (CsA) 0.1% cationic emulsion (CE) in routine clinical practice as a treatment for adults with severe keratitis and dry eye disease (DED) that remained insufficiently controlled with artificial tears. This sub-analysis examined data from ophthalmology clinics in Germany to provide more precise insights into treatment patterns, outcomes, and clinical decision-making related to CsA 0.1% CE.

Methods
Study data were collected from adults starting CsA 0.1% CE (one drop in both eyes at bedtime) and followed up at Week 4, 12, and 24, and Month 12. The primary endpoint was mean change from baseline in corneal fluorescein staining (CFS) score (Oxford Grade Scale) at Month 12. Secondary endpoints examined the severity of ocular signs and symptoms, and adverse events (AEs).

Results
A total of 236 patients from 20 ophthalmology clinics in Germany participated in the PERSPECTIVE study (69.9% female; mean age 60.8 years). Following treatment with CsA 0.1% CE, patients experienced significant reductions in CFS score from Week 4, which were maintained through to Month 12 (P < 0.0001). From baseline, 81.6% of patients experienced an improvement in CFS score at Month 12. CsA 0.1% CE provided significant reductions in the severity of eyelid and conjunctival erythema at Month 12 compared with baseline (P < 0.001), as well as significant reductions in the severity of subjective ocular symptoms (all P ≤ 0.015). Safety data were consistent with the known safety profile of CsA 0.1% CE. Tolerability was rated as “satisfactory,” “good,” or “very good” by 97.2% of physicians and 95.7% of patients.

Conclusion
Outcomes in Germany were similar to those reported for the overall European study population and are indicative of the treatment results that ophthalmologists may expect to see with CsA 0.1% CE treatment in real-life clinical practice. Treatment with CsA 0.1% CE provided long-term improvements over 12 months and was generally well tolerated.
Allele-level visualization of transcription and chromatin by high-throughput imaging
Authors: Almansour Faisal , Keikhosravi Adib , Pegoraro Gianluca , Misteli Tom 
Published: May 2024

AbstractThe spatial arrangement of the genome within the nucleus is a pivotal aspect of cellular organization and function with implications for gene expression and regulation. While all genome organization features, such as loops, domains, and radial positioning, are nonrandom, they are characterized by a high degree of single-cell variability. Imaging approaches are ideally suited to visualize, measure, and study single-cell heterogeneity in genome organization. Here, we describe two methods for the detection of DNA and RNA of individual gene alleles by fluorescence in situ hybridization (FISH) in a high-throughput format. We have optimized combined DNA/RNA FISH approaches either using simultaneous or sequential detection of DNA and nascent RNA. These optimized DNA and RNA FISH protocols were implemented in a 384-well plate format alongside automated image and data analysis and enable accurate detection of individual gene alleles and their gene expression status across a large cell population. We successfully visualized MYC and EGFR DNA and nascent RNA with allele-level resolution in multiple cell types, and we determined the radial position of active and inactive MYC and EGFR alleles. These optimized DNA/RNA detection approaches are versatile and sensitive tools for mapping of chromatin features and gene activity at the single-allele level and at high throughput.
Quantitative assay to analyze neutralization and inhibition of authentic Middle East respiratory syndrome coronavirus
Authors: Müller-Kräuter Helena , Mezzacapo Jolanda , Klüver Michael , Baumgart Sara , Becker Dirk , Fathi Anahita , Pfeiffer Sebastian , Krähling Verena 
Published: May 2024

AbstractTo date, there is no licensed vaccine for Middle East respiratory syndrome coronavirus (MERS-CoV). Therefore, MERS-CoV is one of the diseases targeted by the Coalition for Epidemic Preparedness Innovations (CEPI) vaccine development programs and has been classified as a priority disease by the World Health Organization (WHO). An important measure of vaccine immunogenicity and antibody functionality is the detection of virus-neutralizing antibodies. We have developed and optimized a microneutralization assay (MNA) using authentic MERS-CoV and standardized automatic counting of virus foci. Compared to our standard virus neutralization assay, the MNA showed improved sensitivity when analyzing 30 human sera with good correlation of results (Spearman’s correlation coefficient r = 0.8917, p value < 0.0001). It is important to use standardized materials, such as the WHO international standard (IS) for anti-MERS-CoV immunoglobulin G, to compare the results from clinical trials worldwide. Therefore, in addition to the neutralizing titers (NT50 = 1384, NT80 = 384), we determined the IC50 and IC80 of WHO IS in our MNA to be 0.67 IU/ml and 2.6 IU/ml, respectively. Overall, the established MNA is well suited to reliably quantify vaccine-induced neutralizing antibodies with high sensitivity.
Prognosis of patients with advanced bile tract carcinoma: assessment using the modified-Gustave Roussy Immune Score (mGRIm-s) as a clinico-immunological tool
Authors: Ma Yue , Pan Yuting , Li Yue , Guan Huafang , Dai Guanghai 
Published: May 2024

Abstract
Background
The emergence of immune checkpoint inhibitors (ICIs) has enhanced survival outcomes for certain patients with advanced biliary tract carcinoma (BTC). Pinpointing those who would benefit most from immunotherapy remains elusive. We investigated the predictive value of the modified Gustave Roussy Immune Score (mGRIm-s) in BTC patients treated with ICIs.

Methods
Data from 110 patients at Chinese People's Liberation Army General Hospital, spanning September 2015 to April 2021, were analyzed. The median follow-up duration was 38.7 months as of December 2023. Risk factors included low albumin, high lactate dehydrogenase, and an elevated neutrophil–lymphocyte ratio. Patients were stratified into low (patients with no risk factors) and high (patients with at least one risk factor) mGRIm-s groups based on these factors.

Results
Survival outcomes post-immunotherapy favored the low mGRIm-s group, with significantly improved progression-free survival (PFS) and overall survival (OS) (8.50 months vs. 3.70 months and 21.60 months vs. 8.00 months). COX regression confirmed an elevated risk in the high mGRIm-s group. Subgroup analysis highlighted a notable survival advantage for low mGRIm-s patients receiving first-line immunotherapy.

Conclusions
This study underscores mGRIm-s's potential in predicting immunotherapy response in BTC, paving the way for more targeted approaches.
Planar Drawings with Few Slopes of Halin Graphs and Nested Pseudotrees
Authors: Chaplick Steven , Da Lozzo Giordano , Di Giacomo Emilio , Liotta Giuseppe , Montecchiani Fabrizio 
Published: May 2024

AbstractThe planar slope number$${{\,\textrm{psn}\,}}(G)$$

psn

(
G
)

of a planar graph G is the minimum number of edge slopes in a planar straight-line drawing of G. It is known that $${{\,\textrm{psn}\,}}(G) \in O(c^{\Delta })$$

psn

(
G
)

∈
O

(

c
Δ

)

for every planar graph G of maximum degree $$\Delta $$
Δ
. This upper bound has been improved to $$O(\Delta ^5)$$

O
(

Δ
5

)

if G has treewidth three, and to $$O(\Delta )$$

O
(
Δ
)

if G has treewidth two. In this paper we prove $${{\,\textrm{psn}\,}}(G) \le \max \{4,\Delta \}$$

psn

(
G
)
≤
max
{
4
,
Δ
}

when G is a Halin graph, and thus has treewidth three. Furthermore, we present the first polynomial upper bound on the planar slope number for a family of graphs having treewidth four. Namely we show that $$O(\Delta ^2)$$

O
(

Δ
2

)

slopes suffice for nested pseudotrees.
Climate cyclicity-controlled recurrent bottom-water ventilation events in the aftermath of the Toarcian Oceanic Anoxic Event: the Jenkyns Event
Authors: Schwark Lorenz , Ruebsam Wolfgang 
Published: May 2024

AbstractEnvironmental perturbations of the Toarcian Anoxic Event and its associated carbon isotope excursion (CIE) occurred in a cyclic fashion indicating an orbital control mechanism. Sedimentary strata of the E. elegantulum ammonite subzone in the Lorraine Sub-basin, Luxembourg, exhibit eight sedimentary cycles, most of which postdate the CIE, implying that its termination did not coincide with a full recovery from environmental stress. Sea-level and temperature fluctuations of the Toarcian crisis were linked to a cryosphere demise in the Northern Hemisphere, which modulated stadial versus interstadial phases on the orbital 100 ka eccentricity frequency band. Upon stadial phases, enhanced wind strength in combination with lowered sea level disrupted stratified shelf waters and shifted the storm wave base close to the sea floor. Ventilation of bottom waters interrupted accumulation of laminated and organic-rich black shales, but formed organic-lean and non-laminated gray claystones enriched in terrigenous wax lipids and spores provided via aeolian transport from the hinterland due to the enhanced wind strength. Wind systems are assumed to have been driven by high pressure differences between the cryosphere in the North of the Tethyan shelf and the warmer costal lowland. This distinguishes the atmospheric turbulences after the CIE from the postulated intensification of the cyclones that accompanied the rapid warming at the beginning of the CIE. The deep-water ventilation event following the CIE in the Lorraine Sub-basin was accompanied by a parallel evolution in the SW German Basin, indicating a supraregional driving mechanism in paleobathymetric sub-basins susceptible to lowering of storm wave base. In sub-basins with greater water depth on the NW-Tethyan shelf, post-CIE orbitally driven atmospheric turbulences had a minor or no effect on water column stratification and deposition of organic-rich laminated black shales persisted.
Graphical abstract
Topical antimicrobial treatment of mesh for the reduction of surgical site infections after hernia repair: a systematic review and meta-analysis
Authors: Bontekoning Nathan , Huizing Nathalie J. , Timmer Allard S. , Groenen Hannah , de Jonge Stijn W. , Boermeester Marja A. 
Published: May 2024

Abstract
Purpose
Use of mesh is essential in hernia repair. A common complication after hernia repair is surgical site infection (SSI), which poses a risk in spreading to the mesh, possibly causing mesh infection. Topical antimicrobial pretreatment of mesh may potentially reduce SSI risk in hernia repair and has shown promising results in in vitro and in vivo studies. Clinical evidence, however, is more important. This systematic review aims to provide an overview of available clinical evidence for antimicrobial pretreated mesh in hernia repair surgery to reduce SSI.

Methods
We report in accordance with PRISMA guidelines. CENTRAL, EMBASE, CINAHL and PubMed were searched up to October 2023 for studies that investigated the use of antimicrobial pretreated mesh on SSI incidence in adults undergoing hernia repair. The primary outcome was SSI incidence. We also collected data on pathogen involvement, hernia recurrence, and mesh infection. A meta-analysis on SSI risk and GRADE-assessment was performed of eligible studies.

Results
We identified 11 eligible studies (n = 2660 patients); 5 randomized trials and 6 cohort studies. Investigated interventions included pre-coated mesh, antibiotic carriers, mesh soaked or irrigated with antibiotic or antiseptic solution. Meta-analysis showed no significant reduction in SSI for antibiotic pretreated polypropylene mesh (RR 0.76 [95% CI 0.27; 2.09]; I2 50%).

Conclusion
Data on topical mesh pretreatment to reduce SSI risk after hernia repair is limited. Very low certainty evidence from randomized trials in hernia repair surgery shows no significant benefit for antibiotic mesh pretreatment for SSI reduction, but data are imprecise due to optimal information size not being met.
Diclofenac sodium adsorption in aqueous media by activated carbon obtained from einkorn (Triticum monococcum L.) husk
Authors: Kocabıyık Barış , Üner Osman , Geçgel Ünal 
Published: May 2024

AbstractThe uncontrolled release of waste diclofenac with low biodegradability is considered to be a potential threat for the environment and creatures. To find effective solution for this issue, this study reports the adsorption performance of diclofenac sodium salt (DCF) by using activated carbon (EHAC) obtained from einkorn (Triticum monococcum L.) husk in aqueous solution under various circumstances. It was found that DCF adsorption on EHAC was highly solution pH dependent, and DCF adsorption by EHAC decreased with increasing adsorption temperature. Equilibrium data showed that fitted isotherm model with the experiment results of DCF adsorption on EHAC followed the order of Langmuir > Temkin > Freundlich > Dubinin-Radushkevich. Adsorption capacity of EHAC for DCF adsorption in aqueous solution was calculated to be 147.06 mg/g at 25 °C. The adsorption kinetic of DCF adsorption on EHAC was determined to obey the pseudo-second-order kinetic model. By utilizing FTIR and pH data obtained from DCF adsorption on EHAC, DCF adsorption mechanisms with some interactions such as π-π stacking, electrostatic interactions, and hydrogen bonding were suggested at diverse pH values. Additionally, intraparticle diffusion model was applied to kinetic results to further recognize the kinetic mechanism of DCF adsorption on EHAC. Furthermore, thermodynamic parameters for DCF adsorption on EHAC were calculated and evaluated, in which DCF adsorption process by EHAC was determined to be exothermic, spontaneous, and feasible.
Increases in Awareness and Uptake of Dating Apps’ Sexual Health Features Among US Men Who Have Sex with Men, 2018 to 2021
Authors: Hecht Jennifer , Zlotorzynska Maria , Wohlfeiler Dan , Sanchez Travis H. 
Published: May 2024

AbstractDating apps are now used by the majority of MSM to meet sexual and romantic partners. While research has demonstrated an association between app use and greater number of sex partners and STIs, dating apps also pose an opportunity for intervention. By advocating for new and improved sexual health features on dating apps, Building Healthy Online Communities (BHOC) aims to increase communication about sexual health on the apps. As a follow-up to our previous paper assessing the uptake of sexual health-related profile options on dating apps through Emory’s annual survey of 10,000 MSM in the US, BHOC and Emory partnered to explore the change in uptake over time, again through their annual survey. Among survey participants in 2021, 85% reported using dating apps to meet a partner in the past year, and among this group, 93% reported awareness of sexual health features, up from 77% in 2018 (p < 0.0001). 71% of app users who were aware of features in 2021 reported using one or more sexual health feature, up from 61% in 2018 (p < 0.0001). BHOC will continue to advocate for increased uptake of these features, especially among subgroups with lower levels of uptake.
A survey on imbalanced learning: latest research, applications and future directions
Authors: Chen Wuxing , Yang Kaixiang , Yu Zhiwen , Shi Yifan , Chen C. L. Philip 
Published: May 2024

AbstractImbalanced learning constitutes one of the most formidable challenges within data mining and machine learning. Despite continuous research advancement over the past decades, learning from data with an imbalanced class distribution remains a compelling research area. Imbalanced class distributions commonly constrain the practical utility of machine learning and even deep learning models in tangible applications. Numerous recent studies have made substantial progress in the field of imbalanced learning, deepening our understanding of its nature while concurrently unearthing new challenges. Given the field’s rapid evolution, this paper aims to encapsulate the recent breakthroughs in imbalanced learning by providing an in-depth review of extant strategies to confront this issue. Unlike most surveys that primarily address classification tasks in machine learning, we also delve into techniques addressing regression tasks and facets of deep long-tail learning. Furthermore, we explore real-world applications of imbalanced learning, devising a broad spectrum of research applications from management science to engineering, and lastly, discuss newly-emerging issues and challenges necessitating further exploration in the realm of imbalanced learning.
Aquaculture and Aeromonas hydrophila: a complex interplay of environmental factors and virulence
Authors: Abdella Bahaa , Shokrak Nermeen M. , Abozahra Nourhan A. , Elshamy Yomna M. , Kadira Hossam I. , Mohamed Radi A. 
Published: May 2024

AbstractAquaculture faces significant challenges due to bacterial pathogens like Aeromonas hydrophila, which can severely impact production and fish health. Understanding the relationship between environmental factors, host susceptibility, and bacterial virulence is crucial for effectively managing and mitigating the risks associated with A. hydrophila in aquaculture systems. A. hydrophila, found ubiquitously in aquatic environments, possesses various virulence factors that enhance its pathogenicity. These factors are closely linked to environmental conditions, such as temperature and pH, which play pivotal roles in bacterial growth, survival, and pathogenic potential. Fluctuations in temperature and pH significantly influence A. hydrophila’s metabolic activity and growth rate, thereby modulating its virulence and overall pathogenicity. Ammonia, a byproduct of aquatic organism metabolism and organic matter decomposition, can accumulate to toxic levels in aquaculture settings, compromising fish health and immune function. Elevated ammonia concentrations worsen A. hydrophila infections by compromising host immunity and creating favorable conditions for bacterial proliferation. Oxygen concentration, host signals, and diet formulation are significantly impacting the susceptibility of aquatic organisms to infection. These factors are the most crucial in shaping the ecology, physiology, and pathogenicity of A. hydrophila in aquaculture. There is limited information on how these environmental factors modulate the virulence genes of such important fish pathogens. The knowledge of A. hydrophila virulence and its interplay with environmental factors paves the way for developing strategies to prevent and control diseases in aquaculture.
Preventing Sexual Harm in Nightlife Settings: A Scoping Review
Authors: Button Kira , Taylor Nicholas , Guala Tahnee , De Andrade Dominique , Coomber Kerri , Quigg Zara , Miller Peter 
Published: May 2024

AbstractSexual harm within nightlife settings is a pervasive global concern; however, little is known about the nature of available interventions. The current study aims to review the literature on the nature and effectiveness of nightlife-related sexual harm interventions. A systematic literature search of six databases was conducted to identify records that were published between 1970 and June 2023 and reported approaches that aimed to reduce or prevent nightlife-related sexual harm. Records were included if they theorized, discussed, or evaluated an intervention, prevention or response strategy; however, individual safety strategies were excluded. Results were categorized according to intervention type. Thirty-five peer-reviewed journal articles and 16 gray literature records were identified. The most common nightlife-related sexual harm intervention strategies covered by the literature included targeted policies, laws, and regulations, bystander interventions, and awareness-raising campaigns. Literature in the area is increasing, with the majority of the articles (77.1%) being published in the previous six years; however, there are very few interventions that have been critically evaluated (22.9%). Promising areas for intervention include targeted alcohol regulations (e.g., lockout policies), venue-level policies, and environmental interventions. However, an increase in rigorous evaluative practices is urgently required to ensure future interventions are based on sound theoretical work and empirical evidence.
Intergenerational Transmission of Social Anxiety: The Role of Parents’ Fear of Negative Child Evaluation and Their Self-Referent and Child-Referent Interpretation Biases
Authors: Dülger Melis , Van Bockstaele Bram , Majdandžić Mirjana , de Vente Wieke 
Published: May 2024

Abstract
Purpose
Parents’ fear of negative evaluation (FNE), fear of negative child evaluation (FNCE; parents’ fear that their child is evaluated negatively by others), and self- and child-referent negative interpretation biases have been proposed to play a role in the intergenerational transmission of social anxiety. In this cross-sectional study, we tested whether parents’ self-reported FNE and self-referent interpretation bias, as well as their FNCE and child-referent interpretation bias, statistically mediated the relationship between parent social anxiety and parent-reported child social anxiety.

Methods
A total of 179 parents of 13–16-year-old adolescents completed questionnaires concerning own social anxiety, their FNE, and FNCE and their child’s social anxiety. Parents’ self- and child-referent interpretation biases were measured using scenario completion and memory recognition tasks.

Results
Parents’ FNCE partially statistically mediated the relationship between parent and child social anxiety. Parents’ FNE and their self- and child-referent interpretation biases did, however, not statistically mediate this relationship.

Conclusions
Parents’ FNCE does, but their FNE and self- and child-referent interpretation biases do not seem to play a role in the intergenerational transmission of social anxiety. Hence, parents’ FNCE might be a promising target for clinical practice while designing therapies and interventions concerning child social anxiety.