bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adaptation to Swedish and further development of the ‘Consequences of Screening – Breast Cancer’ questionnaire: a multimethod study
Authors: Bolejko Anetta , Wann‐Hansson Christine , Zackrisson Sophia , Brodersen John , Hagell Peter 
Published: July 2012

Scand J Caring Sci; 2013; 27; 475–486

Adaptation to Swedish and further development of the ‘Consequences of Screening – Breast Cancer’ questionnaire: a multimethod study
Rationale:  Experiencing a false‐positive screening mammography can cause considerable psychosocial distress. The Consequences of Screening – Breast Cancer questionnaire (COS‐BC parts 1 and 2), recently developed in Denmark, is the only condition‐specific questionnaire for measuring short‐ and long‐term psychosocial consequences of false‐positive mammographic screening. Additional studies are needed to further test the COS‐BC before use across cultures. Furthermore, studies have suggested that the consequences of false‐positive screening results are partly common across cancer screening settings, although this hypothesis remains largely untested.Objectives:  This study (i) assesses content validity of a Swedish version of the COS‐BC, (ii) tests whether items expressing long‐term consequences of false‐positive lung cancer screening results are relevant in a breast cancer screening context and (iii) explores the usefulness of taking results from Rasch analyses of the source version as an aid in questionnaire translation and adaptation.Methods:  Following dual‐panel translation, content validity was assessed through qualitative interviews with representatives of the target population and the content validity index (CVI). Item locations and Rasch model fit of the source questionnaires were considered in the translation and assessment process.Results:  The COS‐BC items were generally found relevant and provided coverage of the target construct. Content validity was supported also for nine of 10 lung cancer screening items. Scale CVI values were ≥0.81. Previous Rasch data were useful in facilitating translation and assessing item content validity. The resulting Swedish version of the COS‐BC parts 1 and 2 consists of 34 and 23 items, respectively.Conclusion:  This study illustrates the value of methodological triangulation and use of data from previous Rasch analyses in questionnaire translation and adaptation. We found support for the hypothesis that consequences of false‐positive screening are common across cancer screening settings. Psychometric properties of the Swedish COS‐BC remain to be established.
Haptoglobin is present in albumin used as a replacement solution for plasma exchange
Authors: Cid Joan , Elena Montserrat , Diekmann Fritz , Lozano Miguel 
Published: July 2012

BACKGROUND: Albumin is frequently used as replacement solution when performing plasma exchanges (PEs) and there are no previous data about the content of haptoglobin. The objective was to study the content of haptoglobin in albumin solution and the effect of PE on the removal of haptoglobin, a plasma protein often used for monitoring hemolytic conditions treated with PE.STUDY DESIGN AND METHODS: Haptoglobin was measured in the 5% albumin replacement solution. It was also measured before and after performing 12 PEs using 5% albumin as a replacement solution on four patients. There were three patients with haptoglobin values within the reference range before starting PEs whereas one patient had low levels because of microangiopathic hemolytic anemia.RESULTS: The mean content of haptoglobin in the 5% albumin replacement solution was 0.157 ± 0.005 g/L. Predicted removal according to the one‐compartment model was 70% to 77%. Real removal (RR) of haptoglobin in patients with values within the reference range was 7% to 67%. The RR correlated with the value of haptoglobin before performing PE (r = 0.709; r2 = 0.502; p = 0.03). When haptoglobin levels were low before PE, the levels after the PE were those present in the albumin used as a replacement solution.CONCLUSION: Albumin solution used for PE contained haptoglobin and it explained in part the kinetics removal of plasma haptoglobin observed in the patients.
The new noninvasive occlusion spectroscopy hemoglobin measurement method: a reliable and easy anemia screening test for blood donors
Authors: Pinto Márcio , Barjas‐Castro Maria Lourdes , Nascimento Simone , Falconi Mônica Almeida , Zulli Roberto , Castro Vagner 
Published: July 2012

BACKGROUND: The tests used for anemia screening in blood donors are based on fingerstick samples, leading to discomfort and complaints. The aim of this study was to analyze the feasibility of occlusion spectroscopy method in blood banks and to compare the method with fingerstick hemoglobinometer and hemoglobin (Hb) determination on an automatic blood analyzer.STUDY DESIGN AND METHODS: The study enrolled 205 consecutive volunteer blood donors. Samples were collected by fingerstick and venous punction to determine Hb level by a Hemocue Hb201+ (Hb‐F) and automatic blood analyzer (Hb‐V) and compare to the noninvasive Hb determination by occlusion spectroscopy using NBM200 system (Hb‐NI). The percentage errors of Hb‐F and Hb‐NI of all donors as well as stratified by sex, weight, and age levels were compared to Hb‐V as reference values using Wilcoxon signed rank test.RESULTS: The results obtained with Hb‐F showed significant errors (p < 0.001) in the general group as well as when stratified by sex, weight, and age groups, above values obtained with Hb‐V. Hb‐NI showed significant errors only in females (p = 0.026) and weight level of 61 to 70 kg (p = 0.034), below Hb‐V values.CONCLUSIONS: Hb‐NI seems to be a good method in terms of precision and feasibility for anemia screening of blood donors as well as being much more comfortable for donors.
US public cord blood banking practices: recruitment, donation, and the timing of consent
Authors: Broder Sherri M. , Ponsaran Roselle S. , Goldenberg Aaron J. 
Published: July 2012

BACKGROUND: Cord blood has moved rapidly from an experimental stem cell source to an accepted and important source of hematopoietic stem cells. There has been no comprehensive assessment of US public cord blood banking practices since the Institute of Medicine study in 2005.STUDY DESIGN AND METHODS: Of 34 US public cord blood banks identified, 16 participated in our qualitative survey of public cord blood banking practices. Participants took part in in‐depth telephone interviews in which they were asked structured and open‐ended questions regarding recruitment, donation, and the informed consent process at these banks.RESULTS: Thirteen of 16 participants reported a variably high percentage of women who consented to public cord blood donation. Fifteen banks offered donor registration at the time of hospital admission for labor and delivery. Seven obtained full informed consent and medical history during early labor and eight conducted some form of phased consent and/or phased medical screening and history. Nine participants identified initial selection of the collection site location as the chief mode by which they recruited minority donors.CONCLUSION: Since 2005, more public banks offer cord blood donor registration at the time of admission for labor and delivery. That and the targeted location of cord blood collection sites are the main methods used to increase access to donation and HLA diversity of banked units. Currently, the ability to collect and process donations, rather than donor willingness, is the major barrier to public cord blood banking.
A study of the predonation hemoglobin and iron status among Hong Kong Chinese blood donors
Authors: Lee C.K. , Wong H.K. , Hong J. , Leung J.N.S. , Tsoi W.C. , Lin C.K. 
Published: July 2012

BACKGROUND: Predonation hemoglobin (PDH) is used to safeguard donors’ welfare, and low hemoglobin (Hb) is known to be the most frequent reason for donor deferral. A study was initiated to assess the PDH and iron status of blood donors in Hong Kong.STUDY DESIGN AND METHODS: This observational study was designed with four groups of whole blood donors invited (Group 1—eligible first time donors, Group 2—eligible repeat donors with zero or one donation in preceding 12 months, Group 3—eligible repeat donors with at least two donations in preceding 12 months, Group 4—repeat donors being deferred for low PDH). Predonation blood samples were obtained for blood counts and iron status. Mann‐Whitney test, Kruskal‐Wallis test, and chi‐square test for trend were applied for statistical analysis.RESULTS: A total of 836 donors were recruited, of which 35 were excluded because of hemoglobinopathy. An inverse relationship between serum ferritin level and number of donations in the preceding 12 months was observed in both sexes. Iron deficiency was significantly seen in 35.1% of male and 65.3% of female deferred donors. More importantly, up to 7.2, 5.8, and 29.5% of the female donors in Groups 1, 2, and 3 were found to be iron deficient despite having a high enough PDH.CONCLUSION: This is the first study to assess PDH and iron status in Chinese blood donors. Iron depletion is noted with increasing number of blood donations in the preceding 12 months. Advice on iron repletion is a necessary step for donor welfare and strategies should be developed to ensure that donors have adequate PDH.
Why do so many flour beetle copulations fail?
Authors: Tyler Frances , Tregenza Tom 
Published: July 2012

AbstractCopulations that fail to result in fertilisations are common across a broad range of species, and remain enigmatic given the inherent costs that are being paid by both partners. The determinants of failures are varied and can take effect before, during, or after copulating. In the red flour beetle, Tribolium castaneum (Herbst) (Coleoptera: Tenebrionidae), population estimates of copulations failing to result in fertilisations as high as 55% have been reported, suggesting that the causes of these failures play an important role in the mating system, or that the costs experienced by those determining failure, in this system likely the females, is low. Here, we show that failure is determined peri‐copulation; successful spermatophore transfer (indicated by change in mass of mating individuals) typically results in offspring production, suggesting that mechanisms of post‐copulatory female choice do not cause outright failure. The proportion of copulations that failed to result in fertilisations was apparently not influenced by the relatedness of mating pairs, the age, mating status, or mass of the female, or the interval she experienced between matings. This suggests that alternative adaptive explanations are responsible for the prevalence of copulations that fail to result in fertilisations in this species, or that the costs involved are sufficiently small, so that selection against such failures is weak.
Sclerotic fibroma (storiform collagenoma)‐like stroma in a fibroadenoma of axillary accessory breast tissue
Authors: Val‐Bernal José Fernando , González‐Vela María Carmen , De Grado Mauricio , Garijo María Francisca 
Published: July 2012

AbstractAccessory breast tissue is a subcutaneous remnant persisting after normal embryological development of the breast. It occurs most frequently in the axilla. Fibroadenomas in axillary breast tissue are rare. We report the case of a 29‐year‐old female patient who presented a fibroadenoma arising in the accessory breast tissue of the right axillary fossa. The neoplasm showed foci of sclerotic fibroma‐like stroma. The patient had no signs of Cowden's syndrome. To the best of our knowledge, a lesion of this kind has not been previously reported. This stromal change suggests an uncommon involutional phase of the fibroadenoma with production of sclerotic and hypocellular collagen. The lesion should be differentiated from extraneural perineuroma, from the changes in fibroadenomas in Cowden's syndrome, from sclerosing lobular hyperplasia (fibroadenomatoid mastopathy) and from pseudoangiomatous stromal hyperplasia.
Overexpression of OsPAP10a, A Root‐Associated Acid Phosphatase, Increased Extracellular Organic Phosphorus Utilization in Rice
Authors: Tian Jingluan , Wang Chuang , Zhang Qian , He Xiaowei , Whelan James , Shou Huixia 
Published: July 2012

AbstractPhosphorus (P) deficiency is a major limitation for plant growth and development. Among the wide set of responses to cope with low soil P, plants increase their level of intracellular and secreted acid phosphatases (APases), which helps to catalyze inorganic phosphate (Pi) hydrolysis from organo‐phosphates. In this study we characterized the rice (Oryza sativa) purple acid phosphatase 10a (OsPAP10a). OsPAP10a belongs to group Ia of purple acid phosphatases (PAPs), and clusters with the principal secreted PAPs in a variety of plant species including Arabidopsis. The transcript abundance of OsPAP10a is specifically induced by Pi deficiency and is controlled by OsPHR2, the central transcription factor controlling Pi homeostasis. In gel activity assays of root and shoot protein extracts, it was revealed that OsPAP10a is a major acid phosphatase isoform induced by Pi starvation. Constitutive overexpression of OsPAP10a results in a significant increase of phosphatase activity in both shoot and root protein extracts. In vivo root 5‐bromo‐4‐chloro‐3‐indolyl‐phosphate (BCIP) assays and activity measurements on external media showed that OsPAP10a is a root‐associated APase. Furthermore, overexpression of OsPAP10a significantly improved ATP hydrolysis and utilization compared with wild type plants. These results indicate that OsPAP10a can potentially be used for crop breeding to improve the efficiency of P use.
Knowledge and perspectives of Dutch home healthcare nurses regarding medication frequently used by older people
Authors: Sino Carolien G.M. , Munnik Aukje , Schuurmans Marieke J. 
Published: July 2012

sino c.g.m., munnik a. & schuurmans m.j. (2013) Knowledge and perspectives of Dutch home healthcare nurses regarding medication frequently used by older people. International Journal of Older People Nursing 8, 131–138  doi:10.1111/j.1748‐3743.2012.00336.xBackground.  Home healthcare nurses in their roles as caregivers, educators and administrators of medications are particularly well positioned to act on a preventive way to be alert of adverse drug reactions. However, knowledge about medication and a professional attitude is required.Aim.  To describe medication‐related knowledge and perspectives of Dutch home healthcare nurses regarding frequent used medication by older people.Method.  A cross‐sectional study was conducted among home healthcare nurses (n=146) in the Netherlands based on the ten most frequently used drugs by older people.Finding.  The mean score for total medication knowledge was 76.2% of the maximum score. Most home healthcare nurses (80.3%) felt responsible for improving older patients’ medication use. Three‐quarters of the home healthcare nurses agreed with the statement: “By taking appropriate action at the right time, I am able to prevent a medication‐related hospital admission”.Conclusion.  Although most home healthcare nurses felt responsible for their older patients’ proper medication use and agreed with the statement that they played a role in preventing older patients’ medication‐related hospital admissions, their knowledge regarding medications could be improved.Implications for practice.  Home healthcare nurses should profit as a professional from gaining more knowledge of medication frequently used by older people.
Epidemiology of influenza A (H1N1)pdm09‐associated deaths in the United States, September–October 2009
Authors: Regan Joanna , Fowlkes Ashley , Biggerstaff Matthew , Jhung Michael A. , Gindler Jacqueline , Kennedy Erin , Fields Victoria , Finelli Lyn ,   for the 2009 Pandemic Influenza A (H1N1) Fatal Case Series Investigation Team
Published: July 2012

Please cite this paper as: Regan et al. (2012) Epidemiology of influenza A (H1N1)pdm09‐associated deaths in the United States, September–October 2009. Influenza and Other Respiratory Viruses 6(601), e169–e177.Background  From April to July 2009, the United States experienced a wave of influenza A (H1N1)pdm09 virus (H1N1pdm09) infection. The majority of the deaths during that period occurred in persons <65 years of age with underlying medical conditions.Objective  To describe the epidemiology of H1N1pdm09‐associated deaths in the US during the fall of 2009.Methods  We collected demographic, medical history, and cause of death information on a nationally representative, stratified random sample of 323 H1N1pdm09‐associated deaths that occurred during September 1–October 31, 2009.Results  Data were available for 302/323 (93%) deaths. Most cases (74%) were 18–64 years of age and had ≥1 underlying medical condition (72%). Among cases aged <18 years, 16/43 (37%) had a chronic lung disease, and 15/43 (35%) a neurological disorder; among cases aged ≥18 years, 94/254 (37%) had a chronic lung disease and 84/254 (33%) had a metabolic disorder. The median number of days between symptom onset and death was six among children (range, 1–48) and 12 among adults (range, 0–109). Influenza antiviral agents were prescribed for 187/268 (70%) of cases, but only 48/153 (31%) received treatment within 2 days of illness onset.Conclusions  The characteristics of H1N1pdm09 deaths identified during the fall of 2009 were similar to those occurring April–July 2009. While most cases had conditions that were known to increase the risk for severe outcomes and were recommended to receive antiviral therapy, a minority of cases received antivirals early in the course of illness.
LGR5 is a Marker of Poor Prognosis in Glioblastoma and is Required for Survival of Brain Cancer Stem‐Like Cells
Authors: Nakata Susumu , Campos Benito , Bageritz Josephine , Lorenzo Bermejo Justo , Becker Natalia , Engel Felix , Acker Till , Momma Stefan , Herold‐Mende Christel , Lichter Peter , Radlwimmer Bernhard , Goidts Violaine 
Published: July 2012

AbstractIn various types of cancers including glioblastoma, accumulating evidence show the existence of cancer stem‐like cells (CSCs), characterized by stem cell marker expression, capability of differentiation and self‐renewal, and high potential for tumor propagation in vivo. LGR5, whose expression is positively regulated by the Wnt signaling pathway, is a stem cell marker in intestinal mucosa and hair follicle in the skin. As Wnt signaling is also involved in brain development, the function of LGR5 in the maintenance of brain CSCs is to be assessed. Our study showed that the LGR5 transcript level was increased in CSCs. Co‐immunofluorescence staining demonstrated the co‐localization of CD133‐ and LGR5‐positive cells in glioblastoma tissue sections. Functionally, silencing of LGR5 by lentiviral shRNA‐mediated knockdown induced apoptosis in brain CSCs. Moreover, LGR5 depletion led to a downregulation of L1 cell adhesion molecule expression. In line with an important function in glioma tumorigenesis, LGR5 expression increased with glioma progression and correlated with an adverse outcome. Our findings suggest that LGR5 plays a role in maintenance and/or survival of brain CSCs.
Soil carbon stocks and changes after oil palm introduction in the Brazilian Amazon
Authors: Frazão Leidivan A. , Paustian Keith , Pellegrino Cerri Carlos E. , Cerri Carlos C. 
Published: July 2012

AbstractAs oil palm has been considered one of the most favorable oilseeds for biodiesel production in Brazil, it is important to understand how cultivation of this perennial crop will affect the dynamics of soil organic carbon (SOC) in the long term. The aim of this study was to evaluate the changes in soil C stocks after the conversion of forest and pasture into oil palm production in the Amazon Region. Soil samples were collected in March 2008 and September 2009 in five areas: native forest (NARF), pasture cultivated for 55 years (PAST), and oil palm cultivated for 4 (OP‐4), 8 (OP‐8) and 25 years (OP‐25), respectively. Soils were sampled in March 2008 to evaluate the spatial variability of SOC and nitrogen (N) contents in relation to the spacing between trees. In September 2009, soils were sampled to evaluate the soil C stocks in the avenues (inter rows) and frond piles, and to compare the total C stocks with natural forest and pasture system. Soil C contents were 22–38% higher in the area nearest the oil palm base (0.6 m) than the average across the inter row (0–4.5 m from the tree), indicating that the increment in soil organic matter (SOM) must have been largely derived from root material. The soil C stocks under palm frond piles were 9–26% higher than in the inter rows, due to inputs of SOM by pruned palm fronds. The soil carbon stocks in oil palm areas, after adjustments for differences in bulk density and clay content across treatments, were 35–46% lower than pasture soil C stocks, but were 0–18% higher than the native forest soil C content. The results found here may be used to improve the life cycle assessment of biodiesel derived from palm oil.
Using ecosystem CO2 measurements to estimate the timing and magnitude of greenhouse gas mitigation potential of forest bioenergy
Authors: Bernier Pierre , Paré David 
Published: July 2012

AbstractForest bioenergy opportunities may be hindered by a long greenhouse gas (GHG) payback time. Estimating this payback time requires the quantification of forest‐atmosphere carbon exchanges, usually through process‐based simulation models. Such models are prone to large uncertainties, especially over long‐term carbon fluxes from dead organic matter pools. We propose the use of whole ecosystem field‐measured CO2 exchanges obtained from eddy covariance flux towers to assess the GHG mitigation potential of forest biomass projects as a way to implicitly integrate all field‐level CO2 fluxes and the inter‐annual variability in these fluxes. As an example, we perform the evaluation of a theoretical bioenergy project that uses tree stems as bioenergy feedstock and include multi‐year measurements of net ecosystem exchange (NEE) from forest harvest chronosequences in the boreal forest of Canada to estimate the time dynamics of ecosystem CO2 exchanges following harvesting. Results from this approach are consistent with previous results using process‐based models and suggest a multi‐decadal payback time for our project. The time for atmospheric carbon debt repayment of bioenergy projects is highly dependent on ecosystem‐level CO2 exchanges. The use of empirical NEE measurements may provide a direct evaluation of, or at least constraints on, the GHG mitigation potential of forest bioenergy projects.