bluuebunny/crossref_metadata_2025_split huggingface dataset:
Utility of Intravascular Ultrasound Guidance in Patients Undergoing Percutaneous Coronary Intervention for Type C Lesions
Authors: WAKABAYASHI KOHEI , LINDSAY JOSEPH , LAYNEZ‐CARNICERO ANA , BEN‐DOR ITSIK , SARDI GABRIEL , TORGUSON REBECCA , XUE ZHENYI , SATLER LOWELL F. , PICHARD AUGUSTO D. , WAKSMAN RON 
Published: June 2012

Background:
Percutaneous coronary intervention (PCI) of complex lesions (i.e., American College of Cardiology/American Heart Association class type C) remains challenging and the outcome may be compromised. The use of intravascular ultrasound (IVUS) to guide PCI was suggested to improve outcome.
Methods:

A cohort of 1,984 patients who underwent PCI to type C lesions in our center from April 2000 to March 2010 was identified. Using propensity score matching with clinical and angiographic characteristics, we identified 637 patients who underwent IVUS guidance and 637 patients who had only angiographic guidance PCI. Major adverse cardiovascular events (MACE), a composite end‐point of all‐cause mortality, Q‐wave myocardial infarction and target lesion revascularization, were compared between the 2 groups.
Results:

After propensity score matching, baseline clinical and angiographic characteristics were well matched. Patients undergoing IVUS‐guided PCI had less predilatation and more postdilatation, and were treated more often with cutting balloon. Final diameter stenosis was significantly smaller in the IVUS‐guided group (3 ± 11% vs. 7 ± 19%, P < 0.001), resulting in higher angiographic success compared with the non‐IVUS‐guided group (97.9% vs. 94.8%, P < 0.001). The incidence of MACE was significantly lower in the IVUS‐guided group compared to the angiography‐guided group (11.0% vs. 15.6%, P = 0.017) as was cardiac death (1.9% vs. 4.4%, P = 0.010).
Conclusion:

IVUS‐guided PCI for complex type C lesions is associated with better outcome and should be considered for these lesions. (J Interven Cardiol 2012;25:452–459)
Vascular associated gene variants in patients with preeclampsia: results from the Danish National Birth Cohort
Authors: LYKKE JACOB A. , BARE LANCE A. , OLSEN JØRN , LAGIER ROBERT , TONG CARMEN , ARELLANO ANDRE , PAIDAS MICHAEL J. , LANGHOFF‐ROOS JENS 
Published: June 2012

AbstractObjective. Preeclampsia has been linked to subsequent vascular disease with many shared predisposing factors. We investigated the association between severe preeclampsia, and its subtypes, and specific vascular‐related polymorphisms. Design. The study was a retrospective nested case‐cohort design. Setting. Pregnant Danish women participating in the Danish National Birth Cohort. Population. 263 cases of severe preeclampsia and 1851 random controls were selected from the Danish National Birth Cohort. Methods. We validated all cases of severe preeclampsia and genotyped for 108 single nucleotide polymorphisms (SNPs) that were selected based on previous publications on the association with vascular disease. Logistic models were used for statistical analyses. Main outcome measures. Maternal polymorphisms in genomic models. Results. We found 17 of 108 SNPs associated with severe preeclampsia (p < 0.05). Women homozygous for the rs1799983 in NOS3 were 1.6‐fold [95% confidence interval (CI) 1.0–2.4] more likely to develop severe preeclampsia. Women homozygous for the rs1010 SNP in VAMP8 were twofold (95%CI 1.1–3.5) more likely to deliver preterm when preeclampsia was present. Women homozygous for the rs10811661 SNP were 2.1‐fold (95%CI 1.1–3.9) more likely to develop severe preeclampsia and 3.7‐fold (95%CI 1.1–12.4) more likely to deliver a small‐for‐gestational age child when preeclampsia was present. All associations are available as Supporting Information. Conclusion. We found several vascular‐associated SNPs linked to severe preeclampsia; however, most of these associations are probably by pure chance, which warrants replication and further translational research. To date, no specific SNP has yet proven valuable in a clinical setting in predicting preeclampsia.
Lipid rafts association and anti‐apoptotic function of prohibitin in ultraviolet B light‐irradiated HaCaT keratinocytes
Authors: Wu Qiong , Wu Shiyong 
Published: June 2012

AbstractUpon UVB irradiation, an alternation of major lipid raft components can lead to the recruitment/activation of rafts‐associated proteins and initiation of downstream apoptotic signalling pathways. We used two‐dimensional gel electrophoresis (2‐DE) to identify potential regulators of UVB‐induced apoptosis and mass spectrometry fingerprint analysis to identify proteins that are altered in the rafts after UVB irradiation. Our data show that levels of several proteins, including prohibitin (PHB), were changed in lipid rafts after UVB irradiation. We also demonstrate that while total PHB expression was not changed, the protein was enriched in lipid rafts after UVB irradiation. Reduced expression of PHB using siRNA knockdown resulted in an increase in cellular apoptosis after UVB irradiation. Based on these results, we propose that PHB protects keratinocytes from UVB‐induced apoptosis.
Ultraviolet B‐induced LGI3 secretion protects human keratinocytes
Authors: Lee Seung Hoon , Jeong Yun‐Mi , Kim So‐Young , Jeong Hyo‐Soon , Park Kyoung‐Chan , Baek Kwang Jin , Kwon Nyoun Soo , Yun Hye‐Young , Kim Dong‐Seok 
Published: June 2012

AbstractLeucine‐rich glioma inactivated 3 (LGI3) is known to be expressed mainly in the brain. However, the expression and physiological roles of LGI3 in skin cells remain unknown. In this study, it was found for the first time that LGI3 is expressed mostly by normal human keratinocytes. Furthermore, ELISA analysis showed that HaCaT human keratinocytes increased LGI3 secretion after exposure to ultraviolet B (UVB) in a time‐ and dose‐dependent manner. We next investigated the possible role of LGI3 in keratinocytes. LGI3 (50 ng/ml) increased survival of HaCaT cells by 20% after UVB irradiation (150 mJ/cm2). It was also found that LGI3 stimulates the phosphorylation of Akt, which is involved in the cell survival‐signalling cascade. Furthermore, LGI3 led to the phosphorylation of MDM2 and subsequent p53 degradation. Taken together, the data suggest that LGI3 may regulate p53 levels and that keratinocyte‐derived LGI3 may act as a novel cytokine for skin homoeostasis.
News on microenvironmental physioxia to revisit skin cell targeting approaches
Authors: Grillon Catherine , Matejuk Agata , Nadim Mahdi , Lamerant‐Fayel Nathalie , Kieda Claudine 
Published: June 2012

AbstractThe skin is a multifunctional organ and a first line of defense actively protecting from environmental stress caused by injury, microbial treat, UV irradiation and environmental toxins. Diverse cutaneous cell types together with extracellular matrix elements and factors create a dynamic scene for cellular communication crucial in vital processes such as wound healing, inflammation, angiogenesis, immune response. Direct functional success of skin equilibrium depends on its microenvironment settings and particularly the local oxygen tension. Indeed, skin entire milieu is characterized by and highly dependent on its low oxygen tension called physioxia as emphasized in this review. In the context of skin physioxia, we review and propose here new approaches to minimize age‐related changes in skin state and function. We particularly emphasize carbohydrate‐mediated interactions and new 3D models of engineered skin substitutes. We highlight newly emerged tools and targets including stem cells, miRNAs, matrix metalloproteinases, mitochondria and natural antioxidants that are promising in prevention of skin ageing and disease restraint. In the era of advanced dermatology, new attempts are bringing us closer to ‘well being’ perception.
The influence of surfactants and hydrolyzed proteins on keratinocytes viability and elasticity
Authors: Kobiela Tomasz , Lelen‐Kaminska Karolina , Stepulak Michal , Lekka Malgorzata , Malejczyk Magdalena , Arct Jacek , Majewski Slawomir 
Published: June 2012

Background/purposeThe knowledge how surfactants and hydrolyzed proteins influence the elastic properties of living epidermal keratinocytes is sparse. We demonstrate that the stiffness of cells measured by atomic force microscope (AFM) can be correlated with viability test.Methods and materialsThe effects of sodium lauryl sulphate (SLS) and hydrolyzed collagen (HK) of molecular weight 9 kDa were examined with respect to human keratinocytes viability and elasticity. MTT assay was applied to determine the survival fraction of keratinocytes treated with SLS and HK solutions of various molar ratios. The AFM measurements of the keratinocytes stiffness were carried out immediately after the exposure of cells to the SLS and HK, respectively.ResultsThe increase of the SLS concentration resulted in the decrease of cells proliferation and this effect was inhibited by addition of HK. The strongest inhibition was observed for the SLS:HK molar ratio equals to 2:1. AFM study shows decrease in the cell stiffness for cells treated with SLS. Fluorescence microscopy reveals remodeling of actin filaments of SLS‐treated cells. SLS:HK mixture treatment results in mechanical stiffness close to untreated cells.ConclusionThese results provide possible correlations between mechanical properties and viability of keratinocytes when the chemical stress occurs.
Understorey plant species show long‐range spatial patterns in forest patches according to distance‐to‐edge
Authors: Pellissier Vincent , Bergès Laurent , Nedeltcheva Théodora , Schmitt Marie‐Cécile , Avon Catherine , Cluzeau Catherine , Dupouey Jean‐Luc 
Published: June 2012

AbstractQuestionsHow does the presence of understorey plant species vary with distance‐to‐edge along very large periphery‐to‐interior and forest patch size gradients? Can forest core and periphery species profiles be identified? What life‐history traits can discriminate between forest core and forest periphery species?LocationTemperate forests in the northern half of France.MethodsLocal climate, soil, stand characteristics and landscape metrics were collected on 19 989 plots in 1801 forest patches using data from the French National Forest Inventory. Very large distance‐to‐edge (3–1096 m) and patch size gradients (327–100 000 ha) were explored. Four logistic regression models were compared to determine the response patterns of 214 species to distance‐to‐edge, while controlling for patch size and local habitat quality (soil, climate and stand). The maximum distance of correlation between species occurrence and distance‐to‐edge was assessed using response curve characteristics. The relationships between life‐history traits (habitat preference, preference for ancient forests, reproduction mode, dispersal mode, life form and autecology) and species profile according to distance‐to‐edge were tested.ResultsOf the 214 species analysed, 40 had a core profile and 38 a periphery profile. The maximum distance of correlation was on average 748 m. Core species were more often species reproducing both by seed and vegetatively, ancient forest species, anemochores, bryophytes, pteridophytes, hemicryptophytes and acidophiles, whereas peripheral species were more often species reproducing by seed only, endozoochores, phanerophytes, thermophiles, basophiles, nitrogen‐demanding and heliophiles.ConclusionsSignificant periphery‐to‐core patterns of distribution were detected over much larger ranges than hitherto recognized for common understorey plant species. Plant traits differentiated forest core from forest periphery species. This deep gradient cannot be solely explained by the usual edge‐related biotic and abiotic factors. We hypothesized that it was due to edge displacement following general reforestation since ca. 1830. This edge shift created recent forests with new habitats on former agricultural lands where dispersal‐limited core species had slowly expanded and forest edge species regressed at variable speeds. This long periphery‐to‐interior gradient of presence has important implications for forest plant species distribution, dynamics and conservation.
Changes in circulating adiponectin and metabolic hormone concentrations during periparturient and lactation periods in Holstein dairy cows
Authors: OHTANI Yoshihisa , TAKAHASHI Tatsuyuki , SATO Katsuyoshi , ARDIYANTI Astrid , SONG Sang‐Houn , SATO Reiichiro , ONDA Ken , WADA Yasunori , OBARA Yoshiaki , SUZUKI Keiichi , HAGINO Akihiko , ROH Sang‐gun , KATOH Kazuo 
Published: June 2012

ABSTRACTAlthough our previous report demonstrated that adiponectin and AdipoR1 gene expressions changed among different lactation stages in the bovine mammary gland, its in vivo kinetics remain unclear in ruminant animals. In this study, we investigated the changes in circulating concentrations of adiponectin, as well as other metabolic hormones and metabolites, (i) during the periparturient period and (ii) among different lactation stages, in Holstein dairy cows. In experiment 1, serum adiponectin concentrations increased after parturition. Serum insulin concentrations were lower in the postpartum than prepartum period, whereas serum growth hormone (GH) concentrations increased in the postpartum period. Serum nonesterified fatty acids (NEFA) levels were increased during the postpartum period and were dependent on the parity. In experiment 2, there was no significant difference in plasma adiponectin concentrations among lactational stages. Plasma insulin concentrations tended to be lower in early lactation while plasma GH levels tended to be higher. Plasma NEFA concentrations were significantly lower in mid‐ and late‐lactation stages than non‐lactation stages. These findings indicate that elevation of serum adiponectin might be involved in energy metabolism just around parturition, and might exert its action through regulation of receptor expression levels in target tissues in each lactational stage in Holstein dairy cows.
Factors associated with dry mouth in dependent Japanese elderly
Authors: Kakudate Naoki , Muramatsu Tsukasa , Endoh Mami , Satomura Kazuhito , Koseki Takeyoshi , Sato Yuji , Ito Kayoko , Ogasawara Tadashi , Nakamura Seiji , Kishimoto Etsuo , Kashiwazaki Haruhiko , Yamashita Yoshihisa , Uchiyama Kimio , Nishihara Tatsuji , Kiyohara Yutaka , Kakinoki Yasuaki 
Published: June 2012

ObjectivesTo identify factors associated with dry mouth.BackgroundDry mouth adversely affects oropharyngeal health, particularly in elderly, and can lead to pneumonia. A better understanding of the epidemiology of dry mouth is therefore important in improving treatment strategies and oral health in high‐risk elderly patients.MethodsWe conducted a cross‐sectional study involving 383 dependent Japanese elderly individuals (65–84 [n = 167] and ≥85 [n = 216] years) at eight long‐term care facilities and hospitals. Thirty‐four potential factors associated with dry mouth were examined by multiple logistic regression analysis. The primary outcome was dry mouth, as diagnosed by tongue dorsum moisture.ResultsWe identified that body mass index and severity of physical disability were identified as a potential factors associated with dry mouth in the super‐elderly (≥85 years) group, whereas severity of physical disability, outcome measurement time, high daily water consumption, mouth breathing, use of antidepressants and diuretics, and high frequency of daily brushing (≥2 times per day; Odds ratio: 5.56; 95% Confidence Interval: 1.52–20.00) were associated with dry mouth in the 65‐ to 84‐year‐old group.ConclusionTo our knowledge, this is the first study to identify a link between frequency of daily brushing and dry mouth and suggests that tooth brushing should be encouraged in high‐risk dependent Japanese elderly (65–84 years), particularly those taking antidepressants and/or diuretics.
The Critical Need for Academic Health Centers to Assess the Training, Support, and Career Development Requirements of Clinical Research Coordinators: Recommendations from the Clinical and Translational Science Award Research Coordinator Taskforce*
Authors: Speicher Lisa A. , Fromell Gregg , Avery Sue , Brassil Donna , Carlson Lori , Stevens Erika , Toms Michele 
Published: June 2012

AbstractClinical Research Coordinators (CRCs) are a vital component of the clinical research enterprise providing a pivotal role in human subject protection through the numerous activities and responsibilities assigned to them. In 2006, the National Institutes of Health’s National Center for Research resources (NCRR) implemented the Clinical and Translational Science Awards program (CTSA) to advance biomedical research. As a part of this endeavor, many workgroups were formed among the Consortium to support translational research. The Research Coordinator Taskforce was created as part of the Regulatory Knowledge group of the Clinical Research Innovation Key Function Committee, and focuses on enhancing CTSA capabilities to provide support and training for CRCs. In the spring of 2008, this taskforce conducted two surveys of the then 24 CTSA Consortium members to better understand the current expectations and responsibilities of research coordinators in addition to the mechanism for providing education, training, and support in order for CRCs to successfully meet the study responsibilities placed upon them. The results of these surveys are summarized in this article and provide context to the recommendations of the Research Coordinator Taskforce for institutional considerations, approaches, and best practices for providing education, training, and support the expanding role of CRCs in fulfilling their responsibilities delegated to them by investigators. Clin Trans Sci 2012; Volume 5: 470–475
Category count models for resource management
Authors: Fackler Paul L. 
Published: June 2012

Summary1.A category count model consists of a fixed set of objects (often sites) each of which is classified as one of a set of mutually exclusive categories. Additionally, the category membership of each object evolves over time as a Markov process. The evolution of the objects can be affected by choosing the number of objects in each category that receive alternative management actions.2.Category count models have been used for a variety of resource management applications, including conservation of endangered species, land management and the management of pest infestations.3.This paper provides for the first time a general framework for such models, briefly reviews existing applications that fit the general framework, discusses the non‐trivial problem of how transition probabilities can be computed as well as some of the challenges facing analysts using this framework.4.The framework is applied to an existing application (the management of habitat for a threatened species), demonstrating the importance of modelling the stochasticity inherent in the problem.
Estimating patterns and drivers of infection prevalence and intensity when detection is imperfect and sampling error occurs
Authors: Miller David A. W. , Talley Brooke L. , Lips Karen R. , Campbell Grant Evan H. 
Published: June 2012

Summary1. Epidemiological studies are crucial for understanding the distribution and dynamics of emerging infectious diseases. To accurately assess infection states in wild populations, researchers need to account for observational uncertainty. We focus on two sources of uncertainty when estimating epidemiological parameters: nondetection of infection in sampled individuals and sampling error when quantifying infection intensity for infected individuals.2. We developed new analytical methods to simultaneously estimate prevalence and the distribution of infection intensities based on repeated sampling of individuals in the wild. The methods are an extension of those used for occupancy estimation and address both sources of observation error. At the same time, we account for heterogeneity in detection probability that results from individual variation in infection intensity.3. We use two estimation approaches to account for detection. The first is to use the complete likelihood in a hierarchical Bayesian model and fit using Markov chain Monte Carlo sampling. The second is to estimate the detection relationship using a mark–recapture abundance estimator and use those results to calculate weighted estimates for prevalence and mean infection intensities.4. We use data from a field survey of Batrachochytrium dendrobatidis in Illinois amphibians to test these methods. We show that detection probability using quantitative PCR is strongly related to infection intensity, measured in zoospore equivalents. Sites in the study varied greatly in estimated prevalence and to a lesser extent in mean infection intensities of infected individuals. We did not find evidence of a relationship of snout‐vent‐length to infection intensity or prevalence. Naïve estimates of prevalence that do not account for detection were less than estimates for either of our methods, which yielded similar prevalence values for most sites.5. Uncertainty when assessing disease state is a characteristic of most diagnostic tests. The estimators presented here account for this uncertainty and thus can improve accuracy when assessing the relationship of ecological factors to prevalence and infection intensity.
Null model tests for niche conservatism, phylogenetic assortment and habitat filtering
Authors: Ulrich Werner , Piwczyński Marcin , Maestre Fernando T. , Gotelli Nicholas J. 
Published: June 2012

Summary1. Phylogenetic and trait analyses are powerful tools for disentangling the mechanisms underlying the structure of plant and animal communities, and their use has become prominent in the last decade. However, few studies have simultaneously incorporated data on species traits or phylogeny, environment, and species co‐occurrences. Therefore, the relative importance of these factors as drivers of community assembly is largely unknown.2. We introduce new and conceptually simple null model tests and appropriate metrics to disentangle the relationships between species co‐occurrence, traits or phylogeny and environmental factors not covered by available packages for phylogenetic analysis. We illustrate the methods with an extensive data set on understory plant assemblages sampled in three Polish forests.3. Benchmark testing indicates that the proposed methods have good error behaviour when tested against a variety of artificial matrix sets covering a wide range of observed patterns. Test results are largely independent of matrix size and matrix fill and have adequate power to detect even weak patterns of non‐randomness. The different metrics used are uncorrelated with one another and capture different, and often divergent, patterns expressed within the same matrix.4. Our case study revealed three distinct patterns in forest understory plant assemblages: (i) multiple patterns of species associations within meta‐communities might mask the influence of phylogeny and environmental variables on species occurrences, (ii) the strength of environmental and phylogenetic signals depend on the co‐occurrence pattern (segregated, aggregated, clumped) and might vary within a single meta‐community, and (iii) a random association of phylogeny and species co‐occurrence coupled with significant correlations between environmental factors and phylogeny might reveal species with traits that have passed through environmental filtering.