bluuebunny/crossref_metadata_2025_split huggingface dataset:
Contribution of microwave accelerated distillation in the extraction of the essential oil of Zygophyllum album L.
Authors: Tigrine‐Kordjani Nacéra , Meklati Brahim Youcef , Chemat Farid 
Published: September 2010

AbstractIntroduction – The aerial parts of Zygophyllum album L. are used in folk medicine as an antidiabetic agent and as a drug active against several pathologies. In this work we present the chemical composition of Algerian essential oils obtained by microwave accelerated distillation (MAD) extraction, a solventless method assisted by microwave.Objective – Under the same analytical conditions and using GC‐FID and GC‐MS, the chemical composition of the essential oil of Zygophyllum album L. extracted by MAD was compared with that achieved using hydrodistillation (HD).Methodology – The extracted compounds were hydrosoluble, and they were removed from the aqueous solution by a liquid extraction with an organic solvent.Results – Employing MAD (100°C, 30 min), the essential oil contained mainly oxygenated monoterpenes with major constituents: carvone and α‐terpineol. However, most of the compounds present in the hydrodistilled volatile fraction were not terpene species, with β‐damascenone as a major constituent.Conclusion – The MAD method appears to be more efficient than HD: after 30 min extraction time, the obtained yields (i.e. 0.002%) were comparable to those provided by HD after 3 h extraction. MAD seems to be more convenient since the volatile fraction is richer in oxygenated monoterpenes, species that are recognised for their olfactory value and their contribution to the fragrance of the essential oil. Copyright © 2010 John Wiley & Sons, Ltd.
Hemangioma in the newborn: increased incidence after chorionic villus sampling
Authors: Bauland Constantijn G. , Smit Jeroen M. , Bartelink Lidewij R. , Zondervan Hans A. , Spauwen Paul H. M. 
Published: September 2010

AbstractObjectivesThis study was designed to compare the effects of transcervical chorionic villus sampling (CVS) and amniocentesis on the prevalence of hemangiomas of infancy.MethodsThis is a cohort study of 250 consecutive assessable transabdominal amniocentesis procedures and 250 consecutive assessable transcervical CVS procedures performed between January and September 2002. Parents were asked to fill out a questionnaire regarding the presence of any type of skin lesions. Based on the responses to the questionnaire, children were invited to undergo a physical examination to confirm hemangiomas.ResultsQuestionnaires were returned in 78% of the CVS group (195/250) and in 72% of the amniocentesis group (180/250). Based on the responses in the questionnaire, 78 children in the CVS group and 42 in the amniocentesis group underwent a physical examination. One or more hemangiomas were present in 53 of 195 (27.2%) children in the CVS group versus 17 of 180 (9.4%) children in the amniocentesis group (odds ratio 3.6, 95% CI: 2.0–6.5). There was no difference in congenital abnormalities between the two groups.ConclusionTranscervical CVS is associated with a significantly increased prevalence of hemangiomas compared with amniocentesis. The clinical features of these hemangiomas do not differ from natural hemangiomas and complications of these hemangiomas are very rare. Copyright © 2010 John Wiley & Sons, Ltd.
Diagnostic yield by supplementing prenatal metaphase karyotyping with MLPA for microdeletion syndromes and subtelomere imbalances
Authors: Kjaergaard S. , Sundberg K. , Jørgensen F. S. , Rohde M. D. , Lind A. M. , Gerdes T. , Tabor A. , Kirchhoff M. 
Published: September 2010

AbstractObjectiveThe aim of the study was to retrospectively assess the relevance of using multiplex ligation‐dependent probe amplification (MLPA) for detection of selected microdeletion syndromes (22q11, Prader–Willi/Angelman, Miller–Dieker, Smith–Magenis, 1p‐, Williams), the reciprocal microduplication syndromes and imbalance at the subtelomere regions of chromosomes in a routine prenatal setting.MethodA total of 530 prenatal samples were analysed by commercial MLPA kits (SALSA P064, P036 and P069) in addition to rapid aneuploidy testing and G‐band karyotyping.ResultsAmong the prenatal samples with a normal metaphase karyotype, nine submicroscopic imbalances were detected: seven 22q11 deletions (Velocardiofacial/DiGeorge syndrome), one 15q11deletion (Prader–Willi syndrome) and one terminal deletion of the short arm of chromosome 4 (Wolf–Hirschhorn syndrome). All imbalances were found in amniocentesis (AC) taken due to fetal structural malformation and/or other ultrasound scan (US) detected abnormality. The diagnostic yield was 4.1% in the subgroup with structural malformation and 1.6% in the subgroup with other US abnormality.ConclusionThe data set substantiates that additional MLPA analyses for selected microdeletions and subtelomere imbalances are valuable in routine prenatal diagnostics, when a malformation(s) and/or other abnormalities are detected by US. In contrast, the additional MLPA analyses gave no diagnostic yield in case of increased nuchal translucency (NT). Copyright © 2010 John Wiley & Sons, Ltd.
Proteomics and Down syndrome screening: a validation study
Authors: Koster M. P. H. , Pennings J. L. A. , Imholz S. , Rodenburg W. , Visser G. H. A. , de Vries A. , Schielen P. C. J. I. 
Published: September 2010

AbstractObjectiveIn a previous discovery study, we identified seven potential screening markers for Down syndrome (DS). Here, we report on an extended study to validate the discriminative potential of these markers.MethodsConcentrations of the seven analytes were measured using bead‐based multiplexed immunoassays in maternal serum from 27 DS pregnancies and 27 matched controls. Control samples were matched to the cases by gestational age (exact day), maternal weight ( ± 5 kg), and maternal age ( ± 1 year) and by closest sample date. Prediction values were obtained for current screening markers [pregnancy‐associated plasma protein A (PAPP‐A), free beta human chorionic gonadotrophin (fβ‐hCG) and nuchal translucency (NT)] and seven markers identified before based on concentration fold ratios between DS and controls. Models were fitted based on data of the discovery study or this study and also tested on both datasets.ResultsA significantly higher fold ratio was only found for epidermal growth factor (EGF) (−1.96; p = 0.006). In the prediction model for the current dataset, EGF improved the detection rate (DR) of DS by 5.7% [at a fixed 5% false‐positive rate (FPR)] when added to the currently used screening markers.ConclusionsValidation of previously identified biomarkers only confirmed EGF for further consideration as a DS screening marker. This underlines the importance of validating biomarkers; in this study, limiting the range of plausible biomarkers to only one suitable biomarker. Copyright © 2010 John Wiley & Sons, Ltd.
Assessment of regional right ventricular longitudinal functions in fetus using velocity vector imaging technology
Authors: Pu Da‐rong , Zhou Qi‐chang , Zhang Ming , Peng Qing‐hai , Zeng Shi , Xu Gan‐qiong 
Published: September 2010

AbstractObjectivesThe velocity vector imaging (VVI) technique is useful to assess regional myocardial mechanics. The aim of this study was to evaluate the usefulness of this technology in assessing regional right ventricular longitudinal functions in the fetus and to establish a nomogram of the right ventricle (RV).MethodsWe studied 170 healthy fetuses that were divided into five groups based on gestational age. Dynamic digital images of four chambers were collected and analyzed off‐line. The longitudinal VVI parameters were calculated in the right free wall and ventricular septum, respectively.ResultsA total of 151 out of 170 fetuses (89%) were successfully analyzed using VVI, with good inter‐ and intra‐observer agreements. Normal values for velocity, strain, and strain rate were established. The tissue velocity gradually decreased from basal to apical segment (P < 0.05), whereas strain and strain rate remained stable. The tissue velocity increased with gestational age (P < 0.05), whereas strain and strain rate were stable (P > 0.05).ConclusionFetal myocardial velocity, strain, and strain rate measurements are easy to obtain and are reproducible. From mid‐to‐late gestation, the longitudinal tissue velocity of the RV increases with gestational age, whereas strain and strain rate remain stable. These results indicate that myocardial contractility is established in mid‐gestation and remains constant throughout gestation. Copyright © 2010 John Wiley & Sons, Ltd.
Fetal ocular measurements by MRI
Authors: Li Xiao Bing , Kasprian Gregor , Hodge Jacqueline C. , Jiang Xiao Li , Bettelheim Dieter , Brugger Peter C. , Prayer Daniela 
Published: September 2010

AbstractObjectiveTo present fetal magnetic resonance imaging (MRI) ocular measurement ranges by gestational age (GA) in normal and growth‐restricted fetuses.MethodsA total of 298 pregnant women from the 18th to the 39th week of gestation were imaged using MRI. Ocular measurements including binocular distance (BOD), interocular distance (IOD), transverse ocular diameter (OD) and anterior–posterior (AP) OD were measured. The curve estimation analyses for linear, logarithmic and quadratic models were performed. The ocular measurements of the fetuses with intrauterine growth restriction (IUGR) were compared with that of the normal fetuses.ResultsThe fetal eye resembles an ellipsoid with significantly longer OD and shorter AP (t= − 22.07,p< 0.001). The quadratic model was the best model in predicting growth of the fetal BOD, IOD, OD and AP. The ocular measurements of the fetuses with IUGR were significantly different from that of the normal fetuses (BOD:t= 3.58,p< 0.001; IOD:t= 5.73,p< 0.001; OD:t= 3.52,p< 0.001; AP:t= 2.19,p< 0.05).ConclusionFetal ocular growth can be readily assessed by fetal MRI. Using the normative data provided in this study, fetal ocular anomalies may be detected. Ocular size is frequently reduced in the condition of IUGR, with potential pathologic impact on postnatal vision. Copyright © 2010 John Wiley & Sons, Ltd.
Photovoltaics literature survey (no. 80)
Authors: Shrestha Santosh 
Published: September 2010

AbstractIn order to help keep readers up‐to‐date in the field each issue of Progress in Photovoltaics will contain a list of recently published journal articles most relevant to its aims and scope. This list is drawn from an extremely wide range of journals, including IEEE Transactions on Electron Devices, Journal of Applied Physics, Applied Physics Letters, Progress in Photovoltaics and Solar Energy Materials and Solar Cells. To assist the reader, the list is separated into broad categories, but please note that these classifications are by no means strict. Also note that inclusion in the list is not an endorsement of a paper's quality. If you have any suggestions please email Santosh Shrestha at s.shrestha@unsw.edu.au. magnified image
Evaluation of changes in serum protein profiles during neoadjuvant chemotherapy in HER2‐positive breast cancer using an LC‐MALDI‐TOF/MS procedure
Authors: Mazouni Chafika , Baggerly Keith , Hawke David , Tsavachidis Spyros , André Fabrice , Buzdar Aman U. , Martin Pierre‐Marie , Kobayashi Ryuji , Pusztai Lajos 
Published: September 2010

AbstractComparison of protein profiles of sera acquired before and after preoperative chemotherapy for breast cancer may reveal tumor markers that could be used to monitor tumor response. In this study, we analyzed pre‐ and post‐chemotherapy protein profiles of sera from 39 HER2‐postive breast cancer patients (n=78 samples) who received 6 months of preoperative chemotherapy using LC‐MALDI‐TOF/MS technology. We detected qualitative and quantitative differences in pair‐wise comparison of pre‐ and post chemotherapy samples that were different in patients who achieved pathological complete response (pCR, n=21) compared with those with residual disease (n=18). We identified 2329 and 3152 peaks as differentially expressed in the pre‐chemotherapy samples of the responders and non‐responders. Comparison of matching pre‐ and post‐chemotherapy samples identified 34 (32 decreased, two increased) and 304 peaks (157 decreased, 147 increased) that significantly changed (p<0.01, false discovery rate ≤20%) after treatment in responders and non‐responders, respectively. The top 11 most significantly altered peptide peaks with the greatest change in intensity were positively identified. These corresponded to eight proteins including α‐2‐macroglobulin, complement 3, hemopexin, and serum amyloid P in the responder group and chains C and A of apolipoprotein A‐I, hemopexin precursor, complement C, and amyloid P component in the non‐responding groups. All proteins decreased after therapy, except chain C apolipoprotein A and hemopexin precursor that increased. These results suggest that changes in serum protein levels occur in response to chemotherapy and these changes partly appear different in patients who are highly sensitive to chemotherapy compared with those with lesser response.
Tackling Quantitation: A Report on the Annual Spring Workshop of the HUPO‐PSI 28–30 March 2010, Seoul, South Korea
Authors: Orchard Sandra , Jones Andrew , Albar Juan‐Pablo , Cho Sang Yun , Kwon Kyung‐Hoon , Lee Cheolju , Hermjakob Henning 
Published: September 2010

AbstractThe annual Spring Workshop of the HUPO‐PSI took place in Korea, where the Mass Spectrometry and Protein Separations groups joined forces to tackle the issue of the consistent reporting of quantitative proteomic data generated by mass‐spectrometry‐based technologies. A preliminary mzQuantML schema was drafted which, when completed and tested, will complement the existing mzIdentML schema for reporting protein identifications. The Molecular Interactions group concentrated on the implementations of the PSICQUIC (PSI Common Query InterfaCe) service that allows users to simultaneously query interaction data across multiple participating resources. Work was also undertaken to update the MIAPE guidelines, in response to feedback from the editors of a number of proteomic journals.
Allyl sulfonium salt as a novel initiator for active cationic polymerization of epoxide by shooting with radicals species
Authors: Yilmaz Faruk , Sudo Atsushi , Endo Takeshi 
Published: September 2010

AbstractA rapid cationic polymerization of cyclohexene oxide that completed within a few minutes was achieved by a new initiation system that involves (1) a copper‐catalyzed reduction of benzoyl peroxide by an ascorbic acid derivative that generates free radicals and (2) capture of the radicals by allyl sulfonium salt having hexafluoroantimonate (SbF) as a counter anion, followed by fragmentation of sulfonium radical cation, from which a super acid HSbF6 was produced to initiate the rapid polymerization. The key factor in designing an efficient allyl sulfonium salt was attachment of an electron withdrawing ester group at the allyl group, of which ability to stabilize the formed radical can enhance the efficiency in trapping radicals by the allylic salt. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 4178–4183, 2010
Multistimuli‐responsive hyperbranched poly(ether amine)s
Authors: Yu Bing , Jiang Xuesong , Yin Guilin , Yin Jie 
Published: September 2010

AbstractMultistimuli‐responsive hyperbranched poly(ether amine)s (hPEAs) were successfully synthesized through nucleophilic addition/ring‐opening reaction of commercial diglycidyl ether and amine via one‐pot synthesis. In aqueous solution, these hPEAs exhibited very sharp response to temperature, pH, and ionic strength, with well‐tunable cloud point (CP). Through changing the poly(ethylene oxide) (PEO) chain content of hPEAs, pH, and ionic strength, the CP could be adjustable from 35 to 100 °C, and increased with the increasing of PEO content, the decreasing of pH and ionic strength. The CP of hPEAs aqueous solution presents a linear relationship to the PEO content in pH range from 6.6 to 8.0. Dynamic light scattering (DLS) investigation indicated that these hPEAs dispersed in aqueous solution to form the stable nanomicelles, whose aggregation can be controlled by temperature, pH, and ionic strength. Moreover, the obtained hPEAs contain reactive amino groups in periphery and hydroxyl groups inside, which can be further functionalized. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 48: 4252–4261, 2010
Rare earths/main group metal alkyls catalytic systems for the 1,4‐trans stereoselective coordinative chain transfer polymerization of isoprene
Authors: Valente Andreia , Zinck Philippe , Vitorino Marta J. , Mortreux A. , Visseaux M. 
Published: September 2010

AbstractA series of lanthanum and neodymium complexes comprising the half‐lanthanidocenes Cp*La(BH4)2(THF)2 (Cp* = C5Me5) (1) and Cp*Nd(BH4)2(THF)2 (2), the trisborohydrides La(BH4)3(THF)3 (3) and Nd(BH4)3(THF)3 (4), the trichlorides LaCl3(THF)3 (5) and NdCl3(THF)3 (6), the triisopropoxides La(OiPr)3 (7) and Nd(OiPr)3 (8), and the triaryloxide Nd(OC6H3‐tBu2‐2,6)3 (9) has been assessed for the chain transfer polymerization of isoprene. A transmetalation process is occurring efficiently with the borohydride complexes in the presence of magnesium dialkyl. A gradual decrease of the 1,4‐trans stereoselectivity of the reaction is observed at the benefit of 3,4‐selectivity with increasing quantities of magnesium dialkyl. This can be at least partially attributed to the growth of 3,4 polyisoprene units onto the magnesium atom. By combining dialkylmagnesium and trialkylaluminum, a 1,4‐trans stereospecific reversible coordinative chain transfer polymerization of isoprene is reached using the half‐lanthanocene Cp*La(BH4)2(THF)2. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2010
Synthesis and characterization of poly(isobutylene‐co‐isoprene)‐derived macro‐monomers
Authors: Xiao Shude , Parent J. Scott , Whitney Ralph A. , Knight Lisa K. 
Published: September 2010

AbstractAllylic halide displacement from brominated poly(isobutylene‐co‐isoprene) (BIIR) by carboxylate nucleophiles is used to prepare elastomer derivatives containing pendant polymerizable functionality. These solvent‐borne substitutions are conducted under homogeneous and phase‐transfer catalyzed reaction conditions to synthesize acrylate and vinylbenzoate esters in high yield. The resulting macro‐monomer derivatives are shown to crosslink efficiently with peroxide initiation to give high modulus, thermoset products that cannot otherwise be accessed from isobutylene‐rich elastomers. The extent of cure, as measured by the storage modulus of the vulcanizate, scales with RCH=CH2 content, and can be extended by co‐oligomerization of pendant unsaturation with that contained within multifunctional coagents. An alternate approach involving the introduction of pendant sulfonyl azide functionality is described, wherein thermal decomposition to nitrene intermediates supports an efficient crosslinking process. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2010
Orthogonally functionalizable copolymers based on a novel reactive carbonate monomer
Authors: Cengiz Nergiz , Kabadayioglu Halil , Sanyal Rana 
Published: September 2010

AbstractA novel N‐hydroxy succinimide‐based carbonate monomer that allows direct synthesis of polymers incorporating a reactive carbonate group in the side chain was synthesized. This new monomer was copolymerized with methyl methacrylate and poly(ethylene glycol) methylether methacrylate using free‐radical polymerization to obtain organo‐ and water‐soluble reactive copolymers. Copolymerization of the activated carbonate monomer with an azide‐containing monomer and N‐hydroxy succinimide‐containing activated ester monomer provided orthogonally functionalizable copolymers. The pendant reactive carbonate groups of the copolymers were functionalized with amines to obtain carbamates. Polymers capable of orthogonal functionalization could be selectively functionalized as desired using subsequent 1,3‐dipolar cycloaddition or amidation reactions. The novel monomer and the copolymers were characterized by 1H‐NMR, 13C‐NMR, and infrared spectroscopy. The efficient stepwise orthogonal functionalization of the copolymers were examined via 1H‐NMR spectroscopy. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2010
Multiarm star polymers with peripheral dendritic PMMA arms through Diels–Alder click reaction
Authors: Durmaz Hakan , Dag Aydan , Onen Ceyda , Gok Ozgul , Sanyal Amitav , Hizal Gurkan , Tunca Umit 
Published: September 2010

AbstractDendritic 2‐ and 4‐arm PMMA‐based star polymers with furan‐protected maleimide at their focal point, (PMMA)2n‐MI and (PMMA)4n‐MI were efficiently clicked with the peripheral anthracene functionalized multiarm star polymer, (α‐anthryl functionalized‐polystyrene)m‐poly(divinyl benzene) ((α‐anthryl‐PS)m‐polyDVB) through the Diels–Alder reaction resulting in corresponding multiarm star block copolymers: (PMMA)2n‐(PS)m‐polyDVB and (PMMA)4n‐(PS)m‐polyDVB, respectively. Molecular weights (Mw,TDGPC), hydrodynamic radius (Rh), and intrinsic viscosity (η) of the multiarm star polymers were determined using three‐detection GPC (TD‐GPC). The high efficiency of this methodology to obtain such sterically demanding macromolecular constructs was deduced using 1H‐NMR and UV–vis spectroscopy. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2010
Asymmetric amyloid fibril elongation: A new perspective on a symmetric world
Authors: Heldt Caryn L. , Zhang Shuqi , Belfort Georges 
Published: September 2010

AbstractAmyloids are insoluble, fibrous proteins formed through the aggregation of misfolded proteins. They accumulate in the tissue of individuals with degenerative diseases, such as Parkinson's and Alzheimer's. The purpose of this study was to determine whether fibril growth from an initial model fibril seed is unidirectional or bidirectional. The prevailing theory on amyloid formation is that a symmetric fibril elongates equally from both ends. This study provides evidence to the contrary; the process occurs predominately unidirectionally, demonstrating that amyloid fibrils may be asymmetric and propagate mostly in one direction. Alexa Fluor 568 labeled insulin fibrils were seeded into a native insulin solution and allowed to elongate at 65°C while the kinetics of fibril growth was monitored. The resulting elongated fibrils were labeled with thioflavin‐T, and the fluorescent images of the fibrils show that a majority of the elongated fibrils propagated along only one end of the seed, with the remaining labeled fibrils having bidirectional elongation or no elongation. Using a crystallographic model, we offer a structural explanation for asymmetric growth of the insulin fibrils. Thus, instead of the current view that fibrils grow symmetrically from both ends of the fibril, this is the first evidence that insulin amyloid fibrils formed in solution are asymmetric and appear to grow from only one end. Proteins 2010. © 2010 Wiley‐Liss, Inc.