bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of microstructure and mechanical properties of biodegradable polymer blends of poly(L‐lactic acid) and poly(butylene succinate‐co‐ε‐caprolactone) with lysine triisocyanate
Authors: Vannaladsaysy Vilay , Todo Mitsugu , Jaafar Mariatti , Ahmad Zulkifli , Pasomsouk Korakanh 
Published: April 2010

AbstractEffect of lysine triisocyanate (LTI) as an additive on the microstructure and the mechanical properties of biodegradable polymer blends of poly(L‐lactic acid) (PLLA) and poly(butylene succinate‐co‐ε‐caprolactone) (PBSC) were investigated by using Fourier transform infrared (FTIR) spectroscopy, differential scanning calorimetry (DSC), field‐emission scanning electron microscope (FE‐SEM), and bending and mode I fracture testing techniques. It was found that the addition of LTI dramatically improves the phase‐separation morphology of the PLLA/PBSC blends. FTIR analysis suggested that the NCO groups of LTI were acted as compatibilizer by attributing secondary process between the two polymers PLLA and PBSC, resulting lower peak intensity of ACI samples, understood as secondary process is polar interaction and hydrophobic process. DSC and FE‐SEM results also supported such improvement of immiscibility between PLLA and PBSC. Macroscopic mechanical properties such as the bending fracture energy and the mode I fracture properties are also effectively improved by the LTI addition. Void formation is suppressed due to such morphological change in PLLA/PBSC/LTI blends, and, as a result, energy dissipation in the notch‐tip region of the blends becomes higher than in that of PLLA/PBSC where localized stress concentration due to voids tends to accelerate fracture initiation and, therefore, lowers the fracture energy. POLYM. ENG. SCI., 2010. © 2010 Society of Plastics Engineers
Effect of nanoparticles on the performance of thermally conductive epoxy adhesives
Authors: Fu Jifang , Shi Liyi , Zhang Dengsong , Zhong Qingdong , Chen Yi 
Published: April 2010

AbstractMicrosized or nanosized α‐alumina (Al2O3) and boron nitride (BN) were effectively treated by silanes or diisocyanate, and then filled into the epoxy to prepare thermally conductive adhesives. The effects of surface modification and particle size on the performance of thermally conductive epoxy adhesives were investigated. It was revealed that epoxy adhesives filled with nanosized particles performed higher thermal conductivity, electrical insulation, and mechanical strength than those filled with microsized ones. It was also indicated that surface modification of the particles was beneficial for improving thermal conductivity of the epoxy composites, which was due to the decrease of thermal contact resistance of the filler‐matrix through the improvement of the interface between filler and matrix by surface treatment. A synergic effect was found when epoxy adhesives were filled with combination of Al2O3 nanoparticles and microsized BN platelets, that is, the thermal conductivity was higher than that of any sole particles filled epoxy composites at a constant loading content. The heat conductive mechanism was proposed that conductive networks easily formed among nano‐Al2O3 particles and micro‐BN platelets and the thermal resistance decreased due to the contact between the nano‐Al2O3 and BN, which resulted in improving the thermal conductivity. POLYM. ENG. SCI., 50:1809–1819, 2010. © 2010 Society of Plastics Engineers
Rheology and morphology of polytrimethylene terephthalate/ethylene propylene diene monomer blends in the presence and absence of a reactive compatibilizer
Authors: Aravind Indose , Jose Seno , Ahn Kyung Hyun , Thomas Sabu 
Published: April 2010

AbstractThe dynamic rheological behavior and phase morphology of Polytrimethylene terephthalate (PTT) and ethylene propylene diene monomer (EPDM) uncompatibilized blends and those compatibilized with maleic anhydride grafted EPM (EPM‐g‐MA) were investigated. Effects of blend ratio and reactive compatibilization on the morphology and rheological properties of compatibilized and uncompatibilized blends have been analyzed. The viscosity ratio between the polymers was found to be sensitive to frequency which gave an indirect idea about the unstable morphology. The complex viscosity and dynamic modulus increased with increase in compatibilizer addition up to critical micelle concentration. Palierne and Choi‐Schowalter models were used to calculate the interfacial tension between the polymers. The interfacial tension decreased with the addition of compatibilizer up to CMC. It was also found that the minimum value of interfacial tension was found at CMC beyond that a levelling off is observed. The rheological properties of both compatibilized and uncompatibilized blends are found to be closely related to their phase morphology. POLYM. ENG. SCI., 50:1945–1955, 2010. © 2010 Society of Plastics Engineers
Preparation and characterization of hybrid thiol‐ene/epoxy UV–thermal dual‐cured systems
Authors: Sangermano Marco , Cerrone Monica , Colucci Giovanna , Roppolo Ignazio , Acosta Ortiz Ricardo 
Published: April 2010

AbstractHybrid thiol‐ene/epoxy coatings were prepared by combining thiol‐ene photo‐curable formulations with epoxy monomers, through a dual UV–thermal curing process. An increase in glass transition temperature and in storage modulus was observed for the hybrid thiol‐ene/epoxy coatings when compared with the pristine thiol‐ene UV‐cured system. Also, the bisphenol A moieties introduced into the hybrid networks during the dual‐curing process induced an increase in thermal stability of the cured materials. It has been demonstrated that the addition of epoxy monomer to the thiol‐ene photo‐curable system is a good strategy to follow in order to improve the final properties of thiol‐ene‐based coatings leading to a wide range of possible applications for the hybrid materials. Copyright © 2010 Society of Chemical Industry
Effect of various alumina nano‐fillers on the thermal and mechanical behaviour of low‐density polyethylene–Al2O3 composites
Authors: Malucelli Giulio , Palmero Paola , Ronchetti Silvia , Delmastro Alessandro , Montanaro Laura 
Published: April 2010

AbstractTwo different alumina powders were dispersed in low‐density polyethylene (LDPE) to evaluate if any role can be ascribed to the crystalline phase, size and morphology of the alumina filler. In particular a submicrometric α‐alumina and a nanocrystalline transition (γ/δ) alumina were added to the polymer at 5 wt% concentration, using a Brabender mixing unit. Both the neat inorganic fillers showed a good dispersibility in the polyolefin. The thermal and mechanical properties of the composites obtained were evaluated. As expected, a significant increase of the stiffness and abrasion resistance of LDPE was achieved in both cases. Furthermore, the composites showed a higher thermo‐oxidative stability with respect to neat LDPE. Independent of their crystalline phase, size and morphology, both fillers gave a similar enhancement of composite features. Copyright © 2010 Society of Chemical Industry
Response ofAlvinella pompejanato variable oxygen stress: A proteomic approach
Authors: Mary Jean , Rogniaux Hélène , Rees Jean‐François , Zal Franck 
Published: April 2010

AbstractAlvinella pompejanais one of the most emblematic species of the animal communities colonizing the deep‐sea hydrothermal vents of the East Pacific Rise. This extreme environment is characterized by high temporal variability of its physical–chemical parameters. Among these, the variation in concentration of available oxygen should lead to a specific physiological adaptive response of the animal. To evaluate the mechanisms of this response at a molecular level, a classical 2‐DE‐based proteomic approach has been implemented. After collection (Garrett‐18S and ‐17S vent sites) animals were reconditioned in a high‐pressure chamber pressurized at 260 bar and then the oxygen concentration was regulated to a constant value corresponding to hypoxia, normoxia or hyperoxia for 7 h. The soluble proteins from gills were then analyzed by 2‐DE. The protein content of spots showing specific changes following oxygen concentration variation was determined based on comparison of MS/MS sequence data with a recently establishedA. pompejanaESTs database. Fifteen proteins, belonging mainly to three families, cytoskeleton protein, enzymes of energetic metabolism and heat shock proteins, have been identified as potentially involved in the response to the change in oxygen concentration. The significance of the relatively small set of proteins modulated by oxygen variations is discussed in the context of a potential universal cellular response to stress.
Determination of OH Groups at Plasma Oxidised Poly(propylene) by TFAA Chemical Derivatisation XPS: An Inter‐laboratory Comparison
Authors: Gross Thomas , Pippig Falko , Merz Birgit , Merz Rolf , Vohrer Uwe , Mix Renate , Steffen Hartmut , Bremser Wolfram , Unger Wolfgang E. S. 
Published: April 2010

AbstractA first inter‐laboratory comparison was conducted to demonstrate and document the capability of interested laboratories to measure the fraction of COH species on a plasma oxidised poly(propylene) sample by using a chemical derivatisation XPS approach. This report presents the results from that inter‐laboratory comparison and includes the data received for the measured values and their associated standard deviations of laboratory means, at a 95% confidence level. The measurements were guided by a protocol developed within the group before and summarised shortly in the paper. Standard deviations that may characterise the state‐of‐the‐art for the nominally simple and rather often practised case of TFAA chemical derivatisation XPS of COH species on a plasma oxidised polyolefin surface are calculated following ISO 5725‐2:1994. The main conclusion is that the associated degree of equivalence reached by the participating laboratories in this comparison is still low. Further research to improve chemical derivatisation XPS protocols is mandatory.magnified image
Plasma proteome profiling reveals biomarker patterns associated with prognosis and therapy selection in glioblastoma multiforme patients
Authors: Carlsson Anders , Persson Oscar , Ingvarsson Johan , Widegren Bengt , Salford Leif , Borrebaeck Carl A. K. , Wingren Christer 
Published: April 2010

AbstractPurpose: Glioblastoma multiforme (GBM) is a frequent and aggressive type of primary brain tumor with a heterogeneous origin. GBM is highly therapy resistant and carries a dismal prognosis for the patient. The purpose of this discovery study was to define candidate plasma biomarker signatures for improved classification and novel means for selecting patients for refined individualized therapy.Experimental design: Here, we have for the first time investigated the applicability of large‐scale recombinant antibody‐based microarrays, targeting mainly immunoregulatory analytes, for sensitive and selective plasma protein profiling of GBM patients undergoing immunotherapy with autologous IFN‐γtransfected glioma cells.Results: This proof‐of‐concept study showed that candidate plasma protein signatures associated with GBM were outlined that could be used for GBM classification, monitoring the effects of the immunotherapy as well as for stratifying patients according to the beneficial effect of the adopted immunotherapy. Further, central key cytokines that could be utilized for optimization and/or refinement of the immunotherapeutic regime were indicated.Conclusions and clinical relevance: Candidate plasma proteins signatures associated with GBM was outlined, that could be used for GBM classification and for pre‐operatively stratifying patients according to the beneficial effect of the adopted immunotherapy.
Probing CP violation with kinematic reconstruction at the LHC
Authors: Rolbiecki K. , Moortgat‐Pick G. , Tattersall J. , Wienemann P. 
Published: April 2010

AbstractWe discuss the potential of observing effects of CP‐violation phases in squark decay chains at the LHC [1]. As the CP‐odd observable, we use the asymmetry composed by triple products of final state momenta. There are good prospects of observing these effects using the method of kinematic reconstruction for the final and intermediate state particles. We also discuss the main experimental factors and the expected sensitivity.
Mineralisation of the herbicide linuron by Variovorax sp. strain RA8 isolated from Japanese river sediment using an ecosystem model (microcosm)
Authors: Satsuma Koji 
Published: April 2010

AbstractBACKGROUND: Linuron is a globally used phenylurea herbicide, and a large number of studies have been made on the microbial degradation of the herbicide. However, to date, the few bacteria able individually to mineralise linuron have been isolated only from European agricultural soils. An attempt was made to isolate linuron‐mineralising bacteria from Japanese river sediment using a uniquely designed river ecosystem model (microcosm) treated with 14C‐ring‐labelled linuron (approximately 1 mg L−1).RESULTS: A linuron‐mineralising bacterium that inhabits river sediment was successfully isolated. The isolate belongs to the genera Variovorax and was designated as strain RA8. Strain RA8 gradually used linuron in basal salt medium (5.2 mg L−1) with slight growth. In 15 days, approximately 25% of 14C‐linuron was mineralised to 14CO2, with 3,4‐dichloroaniline as an intermediate. Conversely, in 100‐fold diluted R2A broth, strain RA8 rapidly mineralised 14C‐linuron (5.5 mg L−1) and more than 70% of the applied radioactivity was released as 14CO2 within 3 days, and a trace amount of 3,4‐dichloroaniline was detected. Additionally, the isolate also degraded monolinuron, metobromuron and chlorobromuron, but not diuron, monuron or isoproturon.CONCLUSION: Although strain RA8 can grow on linuron, some elements in the R2A broth seemed significantly to stimulate its growth and ability to degrade. The isolate strictly recognised the structural difference between N‐methoxy‐N‐methyl and N,N‐dimethyl substitution of various phenylurea herbicides. Copyright © 2010 Society of Chemical Industry
Protein immobilization on liposomes and lipid‐coated nanoparticles by protein trans‐splicing
Authors: Chu Nam Ky , Olschewski Diana , Seidel Ralf , Winklhofer Konstanze F. , Tatzelt Jörg , Engelhard Martin , Becker Christian F. W. 
Published: April 2010

AbstractA plethora of methods exist to link proteins to surfaces in order to generate functionalized materials. However, general tools that lead to functional immobilization of recombinantly expressed proteins on membranes such as liposomes or lipid‐coated nanoparticles are rare. Here we present an approach that takes advantage of a double‐palmitoylated peptide that mediates stable membrane anchoring in combination with protein trans‐splicing for efficient immobilization of recombinant proteins fused to split intein segments. Two different DnaE split inteins from Synechocystis and Nostoc punctiforme are tested and compared to immobilization via direct native chemical ligation using a protein thioester. Protein trans‐splicing proceeds at low protein concentrations and leads to functionalized vesicles and membrane‐coated silica nanoparticles. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd.
Study on structure and assembly of the third transmembrane domain of Slc11a1
Authors: Xiao Shuyan , Wang Yuxia , Yang Lei , Qi Haiyan , Wang Chunyu , Li Fei 
Published: April 2010

AbstractThe structure and self‐assembly of the peptide corresponding to the third transmembrane domain (TMD3) of Slc11a1 and its E139A mutant are studied in 1,1,1,3,3,3‐hexafluoro‐2‐propanol (HFIP) aqueous solution by NMR and CD experiments. Slc11a1 is an integral membrane protein with 12 putative TMDs and functions as a pH‐coupled divalent metal cation transporter. Glu139 of Slc11a1 is highly conserved within predicted TMD3 of the Slc11 protein family and function‐associated. Here, we provide the first direct experimental evidence for the structural features of two 24‐residue peptides corresponding to TMD3 of Slc11a1 and its E139A mutant in 60% HFIP‐d2 aqueous solution using CD and NMR spectroscopies. Our study shows that the membrane‐spanning peptide folds as a typical amphipathic α‐helix structure from Ile5 to Met20 with hydrophilic residues Glu12 (Glu139 in Slc11a1) and Asp19 lying on the same side of the helix. The substitution of Glu139 by an alanine residue has little effect on the structure of the peptide, but increases hydrophobicity and facilitates self‐assembly of the peptide. Although the wildtype peptide is monomeric in HFIP aqueous solution, the E139A mutant forms a dimer. The increase in hydrophobicity of the membrane‐spanning peptide and/or change in the interactions between transmembrane segments induced by E139A mutation may affect the metal ion transport of the protein. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd.
Surface photovoltage of Ag nanoparticles and Au chains on Si(111)
Authors: Sell Kristian , Barke Ingo , Polei Stefan , Schumann Christian , von Oeynhausen Viola , Meiwes‐Broer Karl‐Heinz 
Published: April 2010

AbstractWe present surface photovoltage (SPV) measurements of metal nanostructures on Si(111)‐(7 × 7) using scanning tunneling microscopy and spectroscopy (STM/STS). Quantitative results are obtained from spatially resolved I(V) spectra under laser illumination. The SPV data of Ag nanoparticles deposited on Si(111)‐(7 × 7) are compared to results of a Si(111) surface partially covered by the quasi one‐dimensional Si(111)‐(5 × 2)‐Au structure. On the latter we determine the SPV as a function of laser power at different locations. An accurate value for the Fermi‐level pinning position of Si(111)‐(5 × 2)‐Au atomic chains is obtained. Based on these results we establish and discuss a simple model for the band topology. It is confirmed by spatially resolved local work function measurements utilizing the image‐state derived field‐emission resonances.
Stability of disubstituted copper complexes in the gas phase analyzed by electrospray ionization mass spectrometry
Authors: Starke Ines , Kammer Stefan , Holdt Hans‐Jürgen , Kleinpeter Erich 
Published: April 2010

AbstractA series of nitrogen ligand (L)/copper complexes of the type [CuIL]+, [CuIIL(X)]+ and [CuIL2]+ (X = Cl–, BF, acac–, CH3COO– and SO3CF) was studied in the gas phase by electrospray ionization mass spectrometry. The following ligands (L) were employed: 1,12‐diazaperylene (dap), 1,1′‐bisisoquinoline (bis), 2,2′‐bipyridine (bpy), 1,10‐phenanthroline (phen), 2,11‐disubstituted 1,12‐diazaperylenes (dap), 3,3′‐disubstituted 1,1′‐ bisisoquinoline (bis), 5,8‐dimethoxy‐substituted diazaperylene (meodap), 6,6′‐ dimethoxy‐substituted bisisoquinoline (meobis) and 2,9‐dimethyl‐1,10‐phenanthroline (dmphen). Collision‐induced decomposition measurements were applied to evaluate the relative stabilities of the different copper complexes. The influence of the spatial arrangement of the ligands, of the type of substituents and of the counter ion of the copper salts employed for the complexation was examined. Correlations were found between the binding constants of the [ML2]+ complexes in solution and the relative stabilities of the analogous complexes in the gas phase. Furthermore, complexation with the ligands 2,11‐dialkylated 1,12‐diazaperylenes [alkyl = ethyl (dedap) and isopropyl (dipdap)] was studied in the solvents CH3OH and CH3CN. Copyright © 2010 John Wiley & Sons, Ltd.
Highly sensitive and positively charged precolumn derivatization reagent for amines and amino acids in liquid chromatography/electrospray ionization tandem mass spectrometry
Authors: Inagaki Shinsuke , Tano Yuma , Yamakata Yusuke , Higashi Tatsuya , Min Jun Zhe , Toyo'oka Toshimasa 
Published: April 2010

AbstractWe have developed a highly sensitive and positively charged precolumn derivatization reagent, (5‐N‐succinimidoxy‐5‐oxopentyl)triphenylphosphonium bromide (SPTPP), for amines and amino acids in liquid chromatography/electrospray ionization tandem mass spectrometry (LC/ESI‐MS/MS). The handling of the derivatization reaction is quite simple and the reagent reacts with the analytes rapidly and with high efficiency. The derivatized analytes were observed to form regular and intense product ions upon MS/MS analysis; thus, highly sensitive and selective detection was possible in the selected reaction monitoring (SRM) mode. The limits of detection of the SPTPP‐derivatized analytes were less than sub‐femtomole levels. The sensitivities of the derivatized analytes increased about 500‐fold compared to those of underivatized analytes. Since the hydrophobicities of the samples increased after their derivatization, the resolution of the analytes improved dramatically when a reversed‐phase system was used. The relative standard deviations of intra‐day and inter‐day variations were below 10.6% and 13.3%, respectively. The accuracy ranged between 86.6–113% and 83.4–113%, respectively. Furthermore, the developed reagent was used for the analysis of the neurotransmitter 4‐aminobutanoic acid (GABA) and oxidative stress markers such as oxidized, nitrated, and halogenated tyrosines in rat serum. Copyright © 2010 John Wiley & Sons, Ltd.
Fingerprinting of yogurt products by laser desorption spray post‐ionization mass spectrometry
Authors: Liu Jia , Qiu Bo , Luo Hai 
Published: April 2010

AbstractYogurt and related products have been directly analyzed using laser desorption spray post‐ionization mass spectrometry (LDSPI‐MS) in positive ion mode. Assignments are made for some of the abundant diagnostic peaks through LDSPI‐MS/MS analysis in comparison with authentic compounds. It is demonstrated that different yogurt products can be reliably differentiated according to their LDSPI‐MS spectra. Principal component analysis (PCA) is further used to clearly show the capability of LDSPI‐MS fingerprinting for rapid sorting of yogurt products. We believe that this sample‐preparation‐free technique can be a very useful product screening tool in the dairy industry because of its simplicity, reliability and high throughput. Copyright © 2010 John Wiley & Sons, Ltd.