bluuebunny/crossref_metadata_2025_split huggingface dataset:
Vitamin B
12
-Mediated Restoration of Defective Anaerobic Growth Leads to Reduced Biofilm Formation in Pseudomonas aeruginosa
Authors: Lee Kang-Mu , Go Junhyeok , Yoon Mi Young , Park Yongjin , Kim Sang Cheol , Yong Dong Eun , Yoon Sang Sun 
Published: February 2012

ABSTRACT

Pseudomonas aeruginosa
undergoes cell elongation and forms robust biofilms during anaerobic respiratory growth using nitrate (NO
3
−
) as an alternative electron acceptor. Understanding the mechanism of cell shape change induced upon anaerobiosis is crucial to the development of effective treatments against
P. aeruginosa
biofilm infection. Here, we uncovered the molecular basis of anaerobiosis-triggered cell elongation and identified vitamin B
12
to be a molecule that can reinstate defective anaerobic growth of
P. aeruginosa
. The ratio of total cellular DNA content to protein content was significantly decreased in the PAO1 strain grown under anaerobic conditions, indicating that DNA replication is impaired during anaerobic growth. Anaerobic growth of PAO1 reached a higher cell density in the presence of vitamin B
12
, an essential coenzyme of class II ribonucleotide reductase. In addition, cell morphology returned to a normal rod shape and transcription of stress-response genes was downregulated under the same anaerobic growth conditions. These results suggest that vitamin B
12
, the production of which was suppressed during anaerobic growth, can restore cellular machineries for DNA replication and therefore facilitate better anaerobic growth of
P. aeruginosa
with normal cell division. Importantly, biofilm formation was substantially decreased when grown with vitamin B
12
, further demonstrating that anaerobiosis-induced cell elongation is responsible for robust biofilm formation. Taken together, our data reveal mechanistic details of a morphological change that naturally occurs during anaerobic growth of
P. aeruginosa
and illustrates the ability of vitamin B
12
to modulate the biofilm-forming capacity of
P. aeruginosa
under such condition.
Genome Sequence of Sphingomonas sp. Strain PAMC 26605, Isolated from Arctic Lichen (Ochrolechia sp.)
Authors: Shin Seung Chul , Ahn Do Hwan , Lee Jong Kyu , Kim Su Jin , Hong Soon Gyu , Kim Eun Hye , Park Hyun 
Published: February 2012

ABSTRACT

The endosymbiotic bacterium
Sphingomonas
sp. strain PAMC 26605 was isolated from Arctic lichens (
Ochrolechia
sp.) on the Svalbard Islands. Here we report the draft genome sequence of this strain, which could provide further insights into the symbiotic mechanism of lichens in extreme environments.
Genome Sequence of Streptomyces sp. Strain TOR3209, a Rhizosphere Microecology Regulator Isolated from Tomato Rhizosphere
Authors: Hu Dong , Li Xiaozhi , Chang Yueli , He Huan , Zhang Cuimian , Jia Nan , Li Hongtao , Wang Zhanwu 
Published: February 2012

ABSTRACT

Streptomyces
sp. strain TOR3209, isolated from tomato rhizosphere, can regulate the rhizosphere microecology of a variety of crops. Strain TOR3209 could improve plant systemic resistance and promote plant growth. Here, the genome sequence of strain TOR3209 is reported, providing the molecular biological basis of the regulation mechanism of rhizosphere microecology.
Draft Genome Sequence of Marine Streptomyces sp. Strain W007, Which Produces Angucyclinone Antibiotics with a Benz[a]anthracene Skeleton
Authors: Qin Song , Zhang Hongyu , Li Fuchao , Zhu Benwei , Zheng Huajun 
Published: February 2012

ABSTRACTA series of angucyclinone antibiotics have been isolated from marineStreptomycessp. strain W007 and identified. Here, a draft genome sequence ofStreptomycessp. W007 is presented. The genome contains an intact biosynthetic gene cluster for angucyclinone antibiotics, which provides insight into the combinatorial biosynthesis of angucyclinone antibiotics produced by marine streptomycetes.
Complete Genome Sequence of the Highly Hemolytic Strain Bacillus cereus F837/76
Authors: Auger Sandrine , Galleron Nathalie , Ségurens Béatrice , Dossat Carole , Bolotin Alexander , Wincker Patrick , Sorokin Alexei 
Published: February 2012

ABSTRACT

Highly hemolytic strain
Bacillus cereus
F837/76 was isolated in 1976 from a contaminated prostate wound. The complete nucleotide sequence of this strain reported here counts nearly 36,500 single-nucleotide differences from the closest sequenced strain,
Bacillus thuringiensis
Al Hakam. F827/76 also contains a 10-kb plasmid that was not detected in the Al Hakam strain.
Genome Sequence of the Soybean Symbiont Sinorhizobium fredii HH103
Authors: Weidner Stefan , Becker Anke , Bonilla Ildefonso , Jaenicke Sebastian , Lloret Javier , Margaret Isabel , Pühler Alfred , Ruiz-Sainz José E. , Schneiker-Bekel Susanne , Szczepanowski Rafael , Vinardell José María , Zehner Susanne , Göttfert Michael 
Published: February 2012

ABSTRACT

Sinorhizobium fredii
HH103 is a fast-growing rhizobial strain that is able to nodulate legumes that develop determinate nodules, e.g., soybean, and legumes that form nodules of the indeterminate type. Here we present the genome of HH103, which consists of one chromosome and five plasmids with a total size of 7.22 Mb.
Draft Genome Sequence of Clostridium sporogenes PA 3679, the Common Nontoxigenic Surrogate for Proteolytic Clostridium botulinum
Authors: Bradbury Mark , Greenfield Paul , Midgley David , Li Dongmei , Tran-Dinh Nai , Vriesekoop Frank , Brown Janelle L. 
Published: February 2012

ABSTRACT

Clostridium sporogenes
PA 3679 is widely used as a nontoxigenic surrogate for proteolytic strains of
Clostridium botulinum
in the derivation and validation of thermal processes in food. Here we report the draft assembly and annotation of the
C. sporogenes
PA 3679 genome. Preliminary analysis demonstrates a high degree of relatedness between
C. sporogenes
PA 3679 and sequenced strains of proteolytic
C. botulinum
.
Draft Genome Sequence of Pseudomonas psychrotolerans L19, Isolated from Copper Alloy Coins
Authors: Espírito Santo Christophe , Lin Yanbing , Hao Xiuli , Wei Gehong , Rensing Christopher , Grass Gregor 
Published: February 2012

ABSTRACT

We report the draft genome sequence of
Pseudomonas psychrotolerans
strain L19, isolated from a European 50-cent copper alloy coin. Multiple genes potentially involved in copper resistance were identified; however, it is unknown if these copper ion resistance determinants contribute to prolonged survival of this strain on dry metallic copper.
Genome Sequence of the Facultative Anaerobic Arsenite-Oxidizing and Nitrate-Reducing Bacterium Acidovorax sp. Strain NO1
Authors: Huang Yinyan , Li Hang , Rensing Christopher , Zhao Kai , Johnstone Laurel , Wang Gejiao 
Published: February 2012

ABSTRACT

Acidovorax
sp. strain NO1, isolated from gold mine soil, was shown to be a facultative anaerobic arsenite-oxidizing and nitrate-reducing bacterium. The reported draft genome predicts the presence of genes involved in arsenic metabolism, nitrate reduction, phosphate transport, and multiple metal resistances and indicates putative horizontal gene transfer events.
Genome Sequence of the Cycloprodigiosin-Producing Bacterial Strain Pseudoalteromonas rubra ATCC 29570
T
Authors: Xie Bin-Bin , Shu Yan-Li , Qin Qi-Long , Rong Jin-Cheng , Zhang Xi-Ying , Chen Xiu-Lan , Zhou Bai-Cheng , Zhang Yu-Zhong 
Published: February 2012

ABSTRACT

The cycloprodigiosin biosynthetic gene cluster has not been reported. We sequenced the genome of a cycloprodigiosin-producing bacterial strain,
Pseudoalteromonas rubra
ATCC 29570
T
. Analysis revealed a probable cycloprodigiosin biosynthetic cluster, providing a good model for the study of cycloprodigiosin synthesis and regulation.
Draft Genome Sequence of Vibrio fischeri SR5, a Strain Isolated from the Light Organ of the Mediterranean Squid Sepiola robusta
Authors: Gyllborg Mattias C. , Sahl Jason W. , Cronin David C. , Rasko David A. , Mandel Mark J. 
Published: February 2012

ABSTRACT

Here, we describe the draft genome sequence of
Vibrio fischeri
SR5, a squid symbiotic isolate from
Sepiola robusta
in the Mediterranean Sea. This 4.3-Mbp genome sequence represents the first
V. fischeri
genome from an
S. robusta
symbiont and the first from outside the Pacific Ocean.
Dendrochronological evidence of shrub growth suppression by trees in a subarctic lichen woodland
Authors: Boudreau Stéphane , Villeneuve-Simard Marie-Pascale 
Published: February 2012

Expansion of deciduous shrub species in open subarctic landscapes has been reported at several locations around the circumpolar region. However, few data are available to evaluate the response of shrub species in forested ecosystems. For this study, we conducted a dendrochronological analysis to compare the establishment and growth of Betula glandulosa Michx. individuals prior to and after an experimental removal of tree cover (1987) in a lichen woodland located at the boreal forest – tundra ecotone. Tree removal was followed by a B. glandulosa recruitment pulse. It is likely that the newly established seedlings benefited more from heavy trampling during tree removal, which destroyed the lichen cover, than from tree removal itself. A strong growth increase after tree removal suggests that the black spruce tree cover suppressed B. glandulosa growth prior to 1987. Moreover, while radial growth for the period prior to tree removal was not a function of the number of growing degree-days during the growing season, the same climatic variable explained 46% of the growth variability in the second period. These results suggest that the presence of a tree cover might limit the response of shrub species to climate change in forested ecosystems.
Aspects of German military geology and groundwater development in World War II
Authors: Willig D. , Häusler H. 
Published: February 2012

Abstract
A uniformed geological organization was re-created within the German Army by the start of World War II and developed to comprise 40 centres or teams by 1943. Many specialist geotechnical maps and reports prepared by these military geologists have survived the war as part of the Heringen Collection; some remains in the USA, but other parts are in Germany, notably within the archives of the Bundeswehr Geoinformation Office. German armed forces made use of about 400 geologists in total during the conflict, mostly in the Army. Many of their tasks involved groundwater studies, some including the preparation of groundwater prospect maps. Temporary water supplies were set up during mobile campaigns by planning efficient use or enhancement of existing civilian resources, supplemented by driving shallow ‘Abyssinian’ tube wells, for example, in Operation Sea Lion, the invasion of SE England planned for September 1940 but ultimately cancelled. Sustainable long-term supplies in militarily occupied territory were achieved by rigorous data collection and programmes of well drilling, spring capture or percolation gallery construction, one example being on the Channel Islands between 1940 and 1945. Geophysics sometimes aided the geological and borehole studies that guided deployment of well-drilling teams, for example, in 1941/1942, to support German and Italian forces operational in North Africa.
Building an efficient transcoding overlay for P2P streaming to heterogeneous devices
Authors: Liu Dongyu , Li Fei , Shen Bo , Chen Songqing 
Published: February 2012

With the increasing deployment of Internet P2P/overlay streaming systems, more and more clients use mobile devices, such as smart phones and PDAs, to access these Internet streaming services. Compared to wired desktops, mobile devices normally have a smaller screen size, a less color depth, and lower bandwidth and thus cannot correctly and effectively render and display the data streamed to desktops.
To address this problem, in this paper, we propose PAT (Peer-Assisted Transcoding) to enable effective online transcoding in P2P/overlay streaming. PAT has the following unique features. First, it leverages active peer cooperation without demanding infrastructure support such as transcoding servers. Second, as online transcoding is computationally intensive while the various devices used by participating clients may have limited computing power and related resources (e.g., battery, bandwidth), an additional overlay, called metadata overlay, is constructed to instantly share the intermediate transcoding result of a transcoding procedure with other transcoding nodes to minimize the total computing overhead in the system. The experimental results collected within a realistically simulated testbed show that by consuming 6% extra bandwidth, PAT could save up to 58% CPU cycles for online transcoding.
Biomarkers for EGFR-Antagonist Response: In the Genes and on the Genes!
Authors: Easwaran Hariharan , Baylin Stephen B. 
Published: February 2012

Abstract
Patients with non–small cell lung carcinoma containing epidermal growth factor receptor (EGFR)–activating mutations benefit from EGFR-antagonist therapy. EGFR-antagonist sensitivity is also correlated with epithelial–mesenchymal transition, which Walter and colleagues show to be marked by DNA-methylation changes. If validated, these markers could help identify patients with wild-type EGFR who will benefit from EGFR therapy. Clin Cancer Res; 18(8); 2121–3. ©2012 AACR.
microRNA expression signatures and parallels between monocyte subsets and atherosclerotic plaque in humans
Authors: Denecke Bernd , Rostalsky André , Hristov Mihail , Koeppel Thomas A. , Bidzhekov Kiril , Gan Lin , Zernecke Alma , Weber Christian 
Published: February 2012

SummarySmall non-coding microRNAs (miRNAs) have emerged to play critical roles in cardiovascular biology. Monocytes critically drive atherosclerotic lesion formation, and can be subdivided into a classical and non-classical subset. Here we scrutinised the miRNA signature of human classical and non-classical monocytes, and compared miRNA expression profiles of atherosclerotic plaques from human carotid arteries and healthy arteries. We identified miRNAs to be differentially regulated with a two-fold or higher difference between classical and non-classical monocyte subsets. Moreover, comparing miRNA expression in atherosclerotic plaques compared to healthy arteries, we observed several miRNAs to be aberrantly expressed, with the majority of miRNAs displaying a two-fold or higher increase in plaques and only few miRNAs being decreased. To elucidate similarities in miRNA signatures between monocyte subsets and atherosclerotic plaque, expression of miRNAs highly abundant in monocytes and plaque tissues were compared. Several miRNAs were found in atherosclerotic plaques but not in healthy vessels or either monocyte subset. However, we could identify miRNAs co-expressed in plaque tissue and classical monocytes (miR-99b, miR-152), or non-classical monocytes (miR-422a), or in both monocytes subsets. We thus unravelled candidate miRNAs, which may facilitate our understanding of monocyte recruitment and fate during atherosclerosis, and may serve as therapeutic targets for treating inflammatory vascular diseases.Note: The editorial process for this article was fully handled by Prof. G. Y. H. Lip, Editor-in-Chief.
The use of rivaroxaban for short- and long-term treatment of venous thromboembolism
Authors: Cohen Alexander , Dobromirski Mark 
Published: February 2012

SummaryVenous thromboembolism (VTE) is a major healthcare concern and affects more than 1.6 million individuals each year worldwide. Long-term complications include recurrent VTE, chronic thromboembolic pulmonary hypertension and post-thrombotic syndrome. Rivaroxaban is an oral, direct factor Xa inhibitor that has advantages over traditional VTE therapies, including minimal drug and food interactions and no requirement for routine coagulation monitoring. It is currently approved for VTE prevention in adult patients undergoing elective hip or knee replacement surgery. This review evaluates the potential clinical implications of the multicentre, randomised EINSTEIN studies (EINSTEIN DVT and EINSTEIN EXT), which investigated rivaroxaban for the treatment and prevention of recurrent VTE. In EINSTEIN DVT, rivaroxaban was non-inferior to the standard of care (enoxaparin plus a vitamin K antagonist) for recurrent VTE in patients with acute deep-vein thrombosis (DVT) without pulmonary embolism (PE). In EINSTEIN EXT, extended-duration rivaroxaban had superior efficacy to placebo in patients with confirmed DVT or PE who had received 6–12 months of prior VTE treatment. Rivaroxaban was associated with an acceptable safety profile in both studies. The net clinical benefit (efficacy and safety end-points combined) of rivaroxaban was significantly greater than its comparators. The EINSTEIN studies are the first demonstration that a single drug - rivaroxaban - can be effective for both the initial treatment of DVT and prevention of recurrent VTE. Moreover, the simple, once-daily oral administration of rivaroxaban could potentially improve adherence to extended-duration VTE treatment compared with the current standard of care in individuals with confirmed DVT or PE.
Velocity Error for Coherent Doppler Lidar with Pulse Accumulation*
Authors: Frehlich Rod 
Published: February 2012

AbstractThe random estimation or instrument error of coherent Doppler lidar velocity estimates with pulse accumulation (multiple lidar shots per velocity estimate) is determined with computer simulations for general conditions. The sampling errors for overlaid lidar tracks and tropospheric wind field conditions are also calculated for space-based operation. These results permit useful engineering analysis based on the total observation error of the velocity measurements.
Child Labour: Yesterday, Today & Tomorrow
Authors: Padhi P.K. 
Published: February 2012

“I am the child, All the world waits for my coming. All the earth watches with interest to see what I shall become. Civilization hangs in the balance. For what I am, the world of tomorrow will be. I am the child. You hold in your hand my destiny. You determine, largely, whether I shall succeed or fail, Give me, I pray you, these things that make for happiness. Train me, I beg you, that I may be a blessing to the world”. -Mamie Gene Cole Child is the future father and mother, future worker and future citizen of every country, either developed, under developed or developing. The future progress and prosperity of any country depends upon quality and talents of the young children. They are the property of the country and need special care and protection when they become healthy and efficient adults. Well developed children of today, educationally or physically, mean efficient and responsible citizens of tomorrow. Therefore, the problem of child labour in India and the need to protect their rights and status is a very important issue and deserves more attention and consideration by one and all. It is not only the problem of the individual members of society and industry but also of the whole nation. The issue of child labour should be considered as a part of the general problem of human welfare.
Emerging Need for New Breed of Entrepreneurs in India
Authors: Reddy N.R.K. 
Published: February 2012

Winds of Liberalisation blowing across the globe ushered in sweeping changes in every facet of business. Opening up of world economies brought in new opportunities in business. Collapse of command economies, volatile and violent change in business environment caused by quick obsolescence of technology posed new challenges in business. In order to face the fierce competition companies started restructuring their organisations by adapting new technologies to achieve higher productivity and competitiveness. The emergency of new economy brought in structural change in the organisations and its very functioning. This paper discussed the emerging need for new breed of entrepreneurs in the light of change and how emerging change becomes vital in the organisations for success and also discusses the impact of socio-economic and political environment of business and how the environment breeds new class of entrepreneurs.
The Emerging Trends and Practices of HRM
Authors: Mohan A. Chandra , Kumar G. Rajesh 
Published: February 2012

Now-a-day, business organizations are developing very rapidly based on the changing political strategies and market trends. Particularly after globalization, business organizations are facing highly competitive strength and demands due to economic, political, social and technological changes. The uncertainty in the environment and increase of competition have made business organizations to think along new ways of managing business. In order to make organizations more effective, work is restructured like the well-known concepts, QWL (Quality of Work Life), JIT (Just In Time), TQM (Total Quality Management), Team work and Cellular Products. These are introduced continuously based on the changing market trends with a view to fast delivering the goods and for better quality of products/services at convenient and competitive prices. Today, business organizations are restructuring and rethinking with various emerging trends and practices to look at current market trend and moving towards work intensification and flexibility which are ultimately affected by the employer and employee relationship. Temporary and contract jobs, and casual and part-time jobs are the common practice of new management styles. The new trends and practices will ultimately be useful to organizations for decreasing or increasing workforce in order to introduce changes. At the same time, the trend has changed the old concept of permanent employment up to retirement and long time employee and employer relationship. Even some of the advanced countries like USA, and Japan are unable to continue their popular permanent life-time employment due to new trends and practices. Many of the studies have also concluded that temporary employees are increased in many countries. Most of the organizations are providing training programmes for the employees in different levels for the purpose of increasing job knowledge to make them more effective, more productive and multi-skilled. Companies, like Port Trusts (Visakhapatanam unit) which had almost 10000 employees few years ago, recently have about 5000 employees only. Most of the business organizations are planning for right-sizing or down-sizing. In order to make more effective and productive based organizations on the trends and practices in the competitive market, now-a-days, business organizations are planning to practice outsourcing recruitment system, job responsibility sharing, employee referral system, flexible pay structure, flexible time management technique, contract and leasing employees, tele-commuting techniques, etc. All these new trends and practices are highly appreciated and result oriented.