bluuebunny/crossref_metadata_2025_split huggingface dataset:
Revitalisasi permainan tradisional sebagai pengembangan Desa Wisata Muncar
Authors: Rahesti Nonik , Pamungkas Hernandiaz Putra Sri , Irawan Fajar Awang , Bagas Muhammad Khoirudin 
Published: June 2024

Penelitian ini bertujuan untuk mengetahui cara mahasiswa Unnes Giat 6 dalam merevitalisasi permainan tradisional yang ada di Desa Muncar. Penelitian ini menggunakan metode kualitatif, menggunakan data yang diperoleh melalui observasi, wawancara, dan dokumentasi. Responden dalam penelitian ini yaitu perangkat desa dan masyarakat Desa Muncar. Hasil penelitian menunjukan bahwa mahsiswa KKN Unnes Giat 6 memilih dalam merevitalisasi permainan tradisional dengan memberikan peralatan tradisional yang dibuat sendiri bersama bantuan masyarakat Desa Muncar. Simpulan dan saran dalam penelitian ini yaitu penyediaan media permainan tradisional yang dilakukan dalam hal revitalisasi dikatakan berhasil di beberapa dusun di Desa Muncar. Dengan ragam permainan tradisional yang disediakan dengan tujuan dapat melestarikan budaya permainan tradisional di Desa Muncar dapat diterima dengan baik oleh masyarakat khususnya anak-anak Desa Muncar. Dukungan Pemerintah Desa sangat diperlukan untuk pelestarian dan perkembanga dalam menggerakkan masyarakat untuk terus melestarikan budaya permainan tradisional Revitalization of games traditional games as a development of Muncar Tourism VillageThis study aims to find out how Unnes Giat 6 students revitalize traditional games in Muncar Village. This study used qualitative methods, using data obtained through observation, interviews, and documentation. Respondents in this study were village officials and the people of Muncar Village. The results showed that KKN Unnes Giat 6 students chose to revitalize traditional games by providing traditional equipment made by themselves with the help of the Muncar Village community. The conclusions and suggestions in this study are that the provision of traditional game media carried out in terms of revitalization is said to be successful in several hamlets in Muncar Village. With a variety of traditional games provided with the aim of preserving the culture of traditional games in Muncar Village, it can be well received by the community, especially the children of Muncar Village. Village Government support is needed for preservation and development in mobilizing the community to continue to preserve the culture of traditional games.
Contextual Acceptance of COVID-19 Mitigation Mobile Apps in the United States: Mixed Methods Survey Study on Postpandemic Data Privacy
Authors: Feng Yuanyuan , Stenger Brad , Zhang Shikun 
Published: June 2024

Background
The COVID-19 pandemic gave rise to countless user-facing mobile apps to help fight the pandemic (“COVID-19 mitigation apps”). These apps have been at the center of data privacy discussions because they collect, use, and even retain sensitive personal data from their users (eg, medical records and location data). The US government ended its COVID-19 emergency declaration in May 2023, marking a unique time to comprehensively investigate how data privacy impacted people’s acceptance of various COVID-19 mitigation apps deployed throughout the pandemic.

Objective
This research aims to provide insights into health data privacy regarding COVID-19 mitigation apps and policy recommendations for future deployment of public health mobile apps through the lens of data privacy. This research explores people’s contextual acceptance of different types of COVID-19 mitigation apps by applying the privacy framework of contextual integrity. Specifically, this research seeks to identify the factors that impact people’s acceptance of data sharing and data retention practices in various social contexts.

Methods
A mixed methods web-based survey study was conducted by recruiting a simple US representative sample (N=674) on Prolific in February 2023. The survey includes a total of 60 vignette scenarios representing realistic social contexts that COVID-19 mitigation apps could be used. Each survey respondent answered questions about their acceptance of 10 randomly selected scenarios. Three contextual integrity parameters (attribute, recipient, and transmission principle) and respondents’ basic demographics are controlled as independent variables. Regression analysis was performed to determine the factors impacting people’s acceptance of initial data sharing and data retention practices via these apps. Qualitative data from the survey were analyzed to support the statistical results.

Results
Many contextual integrity parameter values, pairwise combinations of contextual integrity parameter values, and some demographic features of respondents have a significant impact on their acceptance of using COVID-19 mitigation apps in various social contexts. Respondents’ acceptance of data retention practices diverged from their acceptance of initial data sharing practices in some scenarios.

Conclusions
This study showed that people’s acceptance of using various COVID-19 mitigation apps depends on specific social contexts, including the type of data (attribute), the recipients of the data (recipient), and the purpose of data use (transmission principle). Such acceptance may differ between the initial data sharing and data retention practices, even in the same context. Study findings generated rich implications for future pandemic mitigation apps and the broader public health mobile apps regarding data privacy and deployment considerations.
Bioactivity and Metabolites Compounds of Medicinal Plants Endophytic Fungi in Indonesia
Authors: Sukmawaty Eka , Karim Abdul , Dwyana Zarsawaty , Natsir Hasnah , Karim Harningsih , Ahmad Ahyar 
Published: June 2024

Indonesia is rich in diversity of medicinal plants, vital in traditional medicine and the pharmaceutical industry. However, overharvesting, along with population growth, land use changes, deforestation, and climate change, endanger these plants. This review investigates the potential of endophytic endosymbiont as an alternative. These endosymbionts can synthesise bioactive compounds similar to those found in medicinal plants. This study compiled data from various sources on endophytic fungi and their bioactivity. The review aims to categorise Indonesian medicinal plants, to identify their associated endophytic fungi from different plant parts, and to assess their bioactivity. The results revealed numerous medicinal plant families and a variety of endophytic fungi isolated from fruits, leaves, twigs, bark, roots, and rhizomes. These fungi exhibited bioactivities, including antioxidant, anticancer, antidiabetic, and antimicrobial effects, with metabolites such as alkaloids, flavonoids, peptides, phenols, polyketides, quinones, steroids, and terpenoids. Fusarium and Colletotrichum were the most common endophytic fungi found. Notably, the biological activity was consistent among endophytic fungi from various host organs, but variations were observed according to the host's geographical origin. This suggests that Indonesia's diverse geography influences metabolite production and activity. However, the same host plant may harbour different species in distinct organs. These findings indicate that endophytic fungi within medicinal plants represent a promising source of bioactive compounds for future Indonesian medicine production. Future research should explore metabolite compounds and bioactivity across different geographical regions.
Species Composition and Inshore Migration of the Tropical Glass Eels (<i>Anguilla</i> spp.) Recruiting to the Jali River, Purworejo Regency
Authors: Indrawati Ayuningtyas , Ibrahim Putri Sapira , Yalindua Fione Yukita , Kurniawan Wanwan , Aisyah Siti , Anggoro Sutrisno , Saputra Suradi Wijaya 
Published: June 2024

Purworejo Regency is located in the south coast of Java where rivers flow into the Indian Ocean. The Jali River in Purworejo becomes a migration route for eels where juvenile eels (glass eels) move from the ocean to freshwater passing through the estuary. During migration, glass eels will make efforts to survive, one of which is to manage their osmotic pressure (osmolarity). With regards to those, a study on the upstream migration of glass eels in Jali River was carried out. Glass eels samplings were conducted using hand-held lift nets at the new moon phase, from 20:00 pm to 4:00 am. Sampling stations included the estuary, a branch river, and below the dam. The osmolarity of both glass eels and media (river water) were measured. Furthermore, the chemical and physical parameters of the waters as the natural habitat for glass eels were also measured. Glass eel samples were then identified to species level. The results showed that glass eels entering the Jali River consisted of Anguilla bicolor bicolor and Anguilla nebulosa nebulosa with the former being the most common. Total length of glass eels ranged between 44.10 to 69.70 mm with total weights 0.08 to 0.54 g. Glass eel migration has a close relation with surface current and tide. The osmotic work level (OWL) of glass eels approaches 0.34 mOsm/l H2O which is close to iso-osmotic. This happened at salinity 0 o/oo so that the glass eels migrate to fresh water which has a lower salinity to achieve iso-osmotic condition.
Evaluation of Temperature Stress Under Different Hydroponic Systems on Growth and Saponin Content of <i>Talinum paniculatum</i> Gaertn. Cuttings
Authors: Yachya Arif , Kristanti Alfinda Novi , Manuhara Yosephine Sri Wulan 
Published: June 2024

Increases in the temperature of nutrient solutions have restricted the use of hydroponic cultivation in the tropics, predominantly due to plant stress. This study aimed to evaluate the effects of temperature stress under different hydroponic systems on the growth and saponin content of Talinum paniculatum cuttings. Three hydroponic systems, i.e., deep flow technique (DFT), nutrient film technique (NFT), and aeroponic, were tested. The temperature of the nutrient solution was set for each system, i.e., under ambient temperature (UAT) and with controlled temperature (WCT) at 26° C. The cultivation period was 60 days. The result showed peroxidation activity and proline accumulation for the adventitious roots of T. paniculatum cuttings with UAT and WCT, alongside various levels of plasma membrane damage. Levels of Malondialdehyde (MDA) and proline were analyzed by spectrophotometer. Membrane damage was analyzed with Evans blue dye. The results indicated that the levels of MDA and proline accumulation under the three hydroponic systems were higher for the WCT than for the UAT treatment. In contrast, vegetative growth was higher in UAT than in WCT. The saponin content of the adventitious root correlated with the MDA level. Saponin production was triggered by oxidative stress during cultivation, while the adventitious roots had a higher saponin content in all three hydroponic systems with the WCT treatment compared to the UAT treatment. Among the systems, aeroponic was superior for biomass and saponin. Root growth was promoted in the nutrient solution under ambient temperature whereas the production of saponins was stimulated under the controlled temperature. In the aeroponic system, root biomass values of 1.17 and 0.478 g dry weight were obtained under ambient and controlled temperatures, respectively. The total saponin contents differed slightly, namely 189.83 and 195.61 mg/g, respectively.
Intraspecific SSR Marker Screening for Detection of <i>Dendrobium crumenatum</i> Mutants Generated from <i>In Vitro</i> Gamma Irradiation
Authors: Dwiyani Rindang , Sanjaya I Putu Wahyu , Yuswanti Hestin , Darmawati Ida Ayu Putri , Suada I Ketut , Manullang Prila Kartika , Fitriani Yuyun 
Published: June 2024

Determination of D. crumenatum mutant obtained from in vitro mutation breeding needs a long time due to its long-life cycle. SSR molecular markers can be used for early mutant detection. Specific SSR markers developed in D. crumenatum are not yet available. Alternative published SSR markers were developed from D. catenatum. The aims of this study are to screen the most informative SSR markers generated from D. catenatum tested in irradiated D. crumenatum population and to determine the gamma irradiation dose resulting the most mutants. Ten SSR markers were randomly selected and tested in 25 individuals of D. crumenatum plantlets irradiated with several doses (0, 5, 10, 15, and 20 Gy; 5 plantlets each dose). The result showed 7 of 10 primers were polymorphic and the other three were monomorphic. All seven polymorphic primers can be used to identified intraspecific variation in the D. crumenatum mutant population. Markers dnsr28 and dnsr98 were the most informative, with the highest polymorphic information content (PIC) value of 0.5. Irradiation D. crumenatum protocorms using 10-15 gray doses were detected as the highest mutant percentage obtained up to 100% in the sample tested. This resulting marker information can be used to screen wider mutant population to decrease the non-mutant individuals in the population for maintenance and cost efficiency. The 10-15 Gy can be used as reference doses for gamma irradiation in 3 months old D. crumenatum protocorm materials.
1-OR: The Improvement Effects of Glucokinase Loss-of-Function Mutation on Lipid Metabolism
Authors: XIE TING , XIE ZIYAN , LIU JING , XIAO XINHUA 
Published: June 2024

Glucokinase-maturity onset diabetes of the young (GCK-MODY), resulting from GCK loss-of-function mutation, is a unique form of diabetes characterized by mild hyperglycemia, favorable lipid profiles, and a low incidence of microvascular and macrovascular complications despite prolonged hyperglycemia. While GCK plays a crucial role in glucose and lipid metabolism, the impact of GCK mutation on lipid metabolism remains elusive. In this study, we aimed to investigate the mechanism underlying the improved lipid metabolism, particularly in liver and adipose tissue, using a mouse model. CRISPR/Cas9 methods was used to generate a heterozygous loss-of-function mutant model by knocking out the GCK gene in C57BL6/J mice. The GCK+/- mice exhibited elevated fasting blood glucose, HDL-c, and ApoA, resembling the phenotype observed in GCK-MODY patients. Metabolic cage analysis revealed a reduced respiratory exchange ratio and increased heat production in GCK+/- mice, suggesting a preference for fat burning over carbohydrate burning compared to WT mice. Additionally, GCK+/- mice showed higher liver and kidney weight (%), but a reduced fat mass (%). Transmission electron microscopy analysis of hepatic tissue showed increased sizes and decreased total volume of lipid droplets (LDs) in GCK+/- mice. Meanwhile, lipidomics analysis of liver revealed significantly reduced levels of TG and DG in the GCK+/- mice. Proteomic data found that several key regulators for LDs lipolysis were reduced in the liver of GCK+/- mice. Immunohistochemistry studies indicated reduced F4/80-positive cells in the liver of GCK+/- mice, suggesting improved hepatic inflammation under normal diet conditions. These findings suggest that partial inactivation of GCK enhances lipid consumption and utilization in the liver and adipose tissue probably through altering LDs formation and lipolysis, providing new insights into the treatment of diabetes and its cardiovascular complications by targeting GCK as a therapeutic target.

Disclosure
T. Xie: None. Z. Xie: None. J. Liu: None. X. Xiao: None.

Funding
National Natural Science Foundation of China (82100901)
1003-P: A New Machine-Learning Model and Expanded Dataset for a Noninvasive BGM
Authors: KLYVE DOMINIC , ANDERSON JAMES H. , CURRIE KAPTAIN , BUI CONNOR , KARIM FAZLE , SOMERS VIREND K. 
Published: June 2024

Introduction: Continuous glucose monitoring (CGM) is essential in diabetes management, but global adoption is hindered due to economic costs and discomfort. A non-invasive, cost-effective, and accurate CGM would support the patient population and increase adoption. This study evaluates the accuracy of a multi-frequency RF sensor for non-invasive blood glucose (BG) monitoring in people with prediabetes and Type 2 diabetes using venous blood as a reference.
Methods: Using a sensor that records data from several thousand radio frequencies (RF), participants’ forearms were scanned during an Oral Glucose Tolerance Test. From 22 participants, 1,430 venous blood samples were collected using a peripheral intravenous catheter. Using the RF data, a CatBoost machine learning (ML) model was built on 80% of these values to estimate BG as a dependent variable. This model was applied to a held-out test dataset and a Mean Absolute Relative Difference (MARD) was calculated.
Results: The CatBoost model returned an overall MARD of 11.8% ± 1.5% on the test dataset. It performed similarly on normoglycemic (12.1% ± 1.8%) and hyperglycemic (11.0% ± 2.3%) ranges. Notably, 100% of predictions fell in Risk Grade A or B in a SEG analysis.
Conclusion: The ML techniques applied to data collected by this RF sensor hold promise for the non-invasive measurement of BG. Ongoing studies will include expanding the participant population and continuing model refinement.

Disclosure
D. Klyve: Consultant; Know Labs, Inc., Zetroz, Inc. J.H. Anderson: Other Relationship; Eli Lilly and Company. Employee; Know Labs Inc. Board Member; Vocare. Employee; PTS Diagnostics. K. Currie: Employee; Know Labs. C. Bui: Employee; Know Labs. F. Karim: Employee; Know Labs. V.K. Somers: Consultant; Know Labs. Research Support; National Institutes of Health. Other Relationship; American Heart Association, American Society of Preventive Cardiology. Consultant; Eli Lilly and Company. Employee; Mayo Clinic. Other Relationship; Medtronic. Research Support; Cambridge Medical.
101-OR: Revelations in Regulation—Sodium/Potassium ATPase Subunit Control of Beta-Cell Cation Handling and Insulin Secretion
Authors: DICKERSON MATTHEW , DADI PRASANNA , JACOBSON DAVID 
Published: June 2024

Background and Aims: Na+/K+ ATPases (NKAs) control ß-cell Ca2+ influx and insulin secretion by integrating the signal strength of stimulatory (Gs) and inhibitory (Gi) G protein-coupled receptors (GPCRs). However, there is a significant gap in our understanding of how specific NKA subunits contribute to ß-cell function. Therefore, due to the importance of NKA-mediated cation handling to islet function, we set out to provide a clear understanding of the roles of NKA subunits in tuning β-cell Ca2+ handling and hormone secretion.
Materials and Methods: Electrophysiology, fluorescence imaging, and hormone secretion assays were employed to study NKA subunit regulation of ß-cell function. A transgenic mouse model with ß-cell knockout of NKAß1 (ßNKAß1KO) was used to determine the importance of this subunit. Human pseudoislets were generated with ß-cell-restricted NKAß1 knockdown (ßNKAß1KD) to investigate the function of this subunit in human islets.
Results: NKAß1 KO increased ß-celI Na+ by 45±1% (P&lt;0.001) and diminished SST-induced NKA currents by 37±6% (P&lt;0.05). Glucose-stimulated Ca2+ influx into ßNKAß1KO islets was delayed by 143±28 seconds (P&lt;0.01) and Ca2+ plateau fraction reduced by 30±3% (P&lt;0.01). ßNKAß1KO mice displayed glucose intolerance (36±17% AUC increase; P&lt;0.5). Glucose-stimulated insulin secretion (GSIS; 9 mM glucose (G)) was decreased by 19±2% from ßNKAß1KO mouse islets (P&lt;0.05) and by 36±11% from human ßNKAß1KD islets (11G; P&lt;0.05). Gi signaling-mediated ßNKAß1KO islet Ca2+ decreases were amplified by 30±7% (P&lt;0.01) and 46±9% (P&lt;0.01) for SSTRs and αADRs respectively. Islet Ca2+ increases due to activation of Gs-coupled GLP1Rs were decreased by 23±6% (P&lt;0.05).
Conclusion: NKAß1 serves a critical role in maintaining the driving force for Na+ entry into ß-cells as well as by shaping the capacity of Gi- and Gs-GPCRs to tune NKA function. Together these findings establish NKAß1 as a key regulator of mouse and human ß-cell function.

Disclosure
M. Dickerson: None. P. Dadi: None. D. Jacobson: None.

Funding
Vanderbilt (ITED T32DK101003R01 DK097392 R01 DK115620)
1011-P: Characterization of Nailfold Capillaries in Patients with Diabetic Ketoacidosis
Authors: XU LAN , HAN YUMENG 
Published: June 2024

Introduction: To evaluate the characteristics of nailfold capillaries in patients with diabetic ketoacidosis (DKA) and to explore the changes in nailfold microcirculation before and after treatment of DKA.
Methods: Following propensity score matching, a total of 34 patients with DKA and 34 patients with diabetes not suffering from DKA were analyzed. Nailfold capillaroscopy (NFC) was applied to photograph and assess the state of the nailfold microcirculation. The arterial blood gas and characterization of nailfold capillaries of patients with DKA were monitored before and after therapy.
Results: Ramified capillary, bizarre capillary and NFC score were significantly higher in the DKA group than the non-DKA group (all P &lt; 0.001). After rehydration and insulin infusion, the density, mean diameter and NFC score were significantly reduced (all P &lt; 0.001). Density correlated positively with blood ketones (r = 0.463, P &lt; 0.001) and negatively with pH (r = -0.368, P = 0.004). NFC score exhibited a positive correlation with anion gap (r = 0.395, P = 0.001) and a negative correlation with pH (r = -0.496, P &lt; 0.001) and actual bicarbonate (r = -0.438, P = 0.001). The area under curve of the regression model including density, diameter and NFC score was 0.839 (P &lt; 0.001).
Conclusion: Nailfold capillaries characteristics vary with DKA, which could enable dynamic monitoring of the therapeutic effects of DKA rehydration.

Disclosure
L. Xu: None. Y. Han: None.
1029-P: Analysis of Raw Data from Freestyle Libre Provides Additional Insights into Diabetic Complication Risk
Authors: HEALD ADRIAN H. , STEDMAN MICHAEL , HABTE-ASRES HELLENA H. , PAISLEY ANGELA N. , WARNER-LEVY JOHN J. , BELSTON LLEYTON , ROBINSON ADAM , JUDE EDWARD B. , GIBSON JOHN M. , FORBES ANGUS 
Published: June 2024

Introduction: Numerous datapoints are collected each day by the FreeStyle Libre system®. We here investigated how further insights can be gained by analysing raw glucose monitoring data.
Methods: Over 18 months, 15minute glucose values were downloaded. Based on these BG values supplementary metrics were calculated including HbA1c. Cluster analysis was used to highlight how these variables add to our understanding of complication risk in T1D and how oral medication impacts these metrics.
Results: for 90 individuals (44 males) were analysed over 18months. Mean age was 42.6(sd 12.7) years; mean duration of diabetes 18.4(sd 11.8) years. Mean BMI 27.8(sd 5.5)kg/m2. Analysis highlighted that average daily variation in BG values was only 20% related to HbA1c. Percentage results above 18mmol/L (top 5% of distribution) increased exponentially above 54mmol/mol HbA1c(7.1%). A greater % of glucose &gt;18mM (Figure) was associated with development of sight threatening retinopathy vs minimal change/no retinopathy (p=0.005) with no relation to glucose variability over 24 hours. Individuals in the lowest tertile of HbA1C at &lt;51.7mmol/mol(%) showed the lowest variation in 24hour glucose profile with less variability in those taking adjunctive oral agents.
Conclusion: Above an HbA1c of 54mmol/mol(7.1%) there is an exponential increase in %glucose &gt;18mM which relates to increased risk of sight threatening retinopathy.

Disclosure
A.H. Heald: None. M. Stedman: None. H.H. Habte-Asres: None. A.N. Paisley: None. J.J. Warner-Levy: None. L. Belston: None. A. Robinson: None. E.B. Jude: Speaker's Bureau; AstraZeneca. Research Support; Novo Nordisk. Speaker's Bureau; Lilly Diabetes. Research Support; Abbott. Speaker's Bureau; Sanofi. J.M. Gibson: None. A. Forbes: None.