bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of the possible role of prostaglandin F2α in laminitis induced in horses by nasogastric administration of black walnut heartwood extract
Authors: Noschka Erik , Moore James N. , Peroni John F. , Lewis Tristan H. , Lewis Stephen J. , Robertson Tom P. 
Published: February 2010

Abstract
Objective—To provide insights into the role of prostaglandin F2α (PGF2α) in the developmental stages of laminitis induced in horses by ingestion of black walnut heartwood extract (BWHE).
Sample Population—10 adult mixed-breed horses.
Procedures—Horses were separated into 2 groups and were euthanatized at 12 hours after placebo (water) administration (control horses) or after BWHE administration and development of Obel grade 1 laminitis. Blood samples were obtained to determine plasma PGF2α concentrations hourly for the first 4 hours and subsequently every 2 hours after substance administration. Laminar arteries and veins were isolated, and responses to increasing concentrations of PGF2α were measured before and after preincubation of blood vessels with prostanoid and thromboxane receptor antagonists SQ 29,548, SC-19220, and AH 6809.
Results—Plasma PGF2α concentrations increased in horses given BWHE; the WBC count decreased concurrently. In control horses, PGF2α was a potent contractile agonist for laminar veins but not for laminar arteries. In horses given BWHE, PGF2α was similarly selective for laminar veins; however, the magnitude of PGF2α-induced venoconstriction was less than that in control horses. After preincubation with SQ 29,548, laminar veins from control horses responded to PGF2α with a small degree of dilation, whereas laminar veins from horses given BWHE did not.
Conclusions and Clinical Relevance—PGF2α may play a role in the inflammatory and vascular dysfunction associated with the prodromal stages of laminitis. Prostanoids such as PGF2α may be viable targets for the prevention of acute laminitis in horses.
Evaluation of innervation of the mitral valves and the effects of myxomatous degeneration in dogs
Authors: Culshaw Geoff J. , French Anne T. , Han Richard I. , Black Alexander , Pearson Geoff T. , Corcoran Brendan M. 
Published: February 2010

Abstract
Objective—To map aspects of the innervation of the mitral valve complex and determine any association with the development or progression of myxomatous mitral valve disease (MMVD) in dogs.
Sample Population—Septal mitral valve leaflets from 11 dogs aged 6 months to > 10 years.
Procedures—Expression of protein gene product 9.5 (general neuronal marker), tyrosine hydroxylase (adrenergic innervation marker), vasoactive intestinal peptide (parasympathetic innervation marker), and calcitonin gene–related peptide (sensory innervation marker) was assessed by use of a standard immunohistochemical technique. Innervation was assessed qualitatively and semiquantitatively. Differences between valvular zones and between groups were analyzed statistically.
Results—MMVD was present in leaflets of all dogs ≥ 5 years of age. Innervation was confirmed in all leaflets but was markedly reduced in leaflets of dogs > 10 years of age. Innervation was most dense at the base of valves and mainly associated with the epimysial, perimysial, and endomysial layers of the muscle and blood vessels within the valve. Innervation was reduced within the middle zone of the valve and lacking at the free edge. Innervation was not identified at the tip of the leaflet, the free edge, or the chordae. Nerve fibers were mostly sympathetic, with the remainder being parasympathetic or sensory. Existence of MMVD did not alter the pattern or density of innervation.
Conclusions and Clinical Relevance—Mitral valve leaflets in the study dogs were innervated, with most of the nerve fibers associated with the myocardium in the valve base. Development of MMVD appeared to precede the reduction of innervation associated with advancing age.
Evaluation of the analgesic effect of intracameral lidocaine hydrochloride injection on intraoperative and postoperative pain in healthy dogs undergoing phacoemulsification
Authors: Park Shin Ae , Park Young Woo , Son Won Gyun , Kim Tae Hyun , Ahn Jae Sang , Ahn Jeong Taek , Kim Se Eun , Lee Inhyung , Seo Kangmoon 
Published: February 2010

Abstract
Objective—To evaluate the intraoperative and postoperative analgesic effects of intracameral lidocaine hydrochloride injection in dogs undergoing phacoemulsification.
Animals—12 healthy Beagles with healthy eyes.
Procedures—Dogs were randomly assigned to receive 1 of 2 intracameral injections: 2% lidocaine hydrochloride solution (0.3 mL) or an equivalent amount of balanced salt solution (BSS). All dogs were treated with acepromazine (0.05 mg/kg, IV) and cefazolin (30 mg/kg, IV), and tropicamide drops were topically applied to the eyes. Anesthesia was induced with propofol and maintained with isoflurane. The initial end-tidal isoflurane concentration was maintained at 1.2%. Heart rate, respiratory rate, arterial blood pressure, esophageal temperature, inspired and end-tidal isoflurane concentrations, and oxygen saturation were recorded every 5 minutes. The allocated agent was injected intracamerally after aspiration of the same volume of aqueous humor. Ten minutes after injection, phacoemulsification was performed. After surgery began, the isoflurane concentration was adjusted according to heart rate and mean arterial blood pressure. Pain scores were recorded before surgery and at 0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 6, 8, 16, and 24 hours after extubation.
Results—Isoflurane requirements were significantly higher in the BSS group than in the lidocaine group. Mean ± SD time to administration of supplementary analgesia was significantly shorter in the BSS group (1.4 ± 1.2 hours) than in the lidocaine group (4.9 ± 1.2 hours).
Conclusions and Clinical Relevance—Intracameral lidocaine injection had significant analgesic effects in dogs undergoing cataract surgery. Results of this study suggest the value of intracameral lidocaine injection as an analgesic for intraocular surgery in dogs.
Effect of treatment with a topical ophthalmic preparation of 1% nalbuphine solution on corneal sensitivity in clinically normal horses
Authors: Wotman Kathryn L. , Utter Mary E. 
Published: February 2010

Abstract
Objective—To assess the effect of treatment with a topical ophthalmic preparation of 1.2% nalbuphine solution on corneal sensitivity in clinically normal horses.
Animals—8 horses.
Procedures—Baseline corneal touch threshold (CTT) was measured (defined as the mean filament length [mm] at which a consistent blink response was elicited) for both eyes of each horse by use of a Cochet-Bonnet aesthesiometer. Subsequently, 0.2 mL of 1.2% nalbuphine solution was instilled in 1 randomly selected eye of each horse, and 0.2 mL of artificial tears solution was instilled in the contralateral eye (control treatment). For all 8 horses, CTT of each eye was measured within 1 minute following nalbuphine or artificial tears administration and every 15 minutes thereafter for 60 minutes. For 5 of the 8 horses, CTT was also measured in both eyes at 120 minutes. Changes in CTT values from baseline over time were assessed, as were differences between treated and control eyes.
Results—At any time point, corneal sensitivity following nalbuphine treatment did not differ significantly from control treatment findings. Mean CTTs for nalbuphine-treated and control eyes were 38.8 and 37.9 mm, respectively. In both groups, CTT was significantly lower than baseline value at 15, 45, 60, and 120 minutes. No tearing or redness developed in any eye treated with nalbuphine.
Conclusions and Clinical Relevance—Topical administration of ophthalmic 1% nalbuphine solution had no effect on corneal sensitivity in clinically normal horses. The topical ocular treatment was not associated with local irritation.
Effects of unfocused extracorporeal shock wave therapy on healing of wounds of the distal portion of the forelimb in horses
Authors: Silveira Andressa , Koenig Judith B. , Arroyo Luis G. , Trout Donald , Moens Noël M. M. , LaMarre Jonathan , Brooks Andrew 
Published: February 2010

Abstract
Objective—To determine effects of extracorporeal shock wave therapy (ESWT) on healing of wounds in the distal portion of the forelimb in horses.
Animals—6 horses.
Procedures—Five 6.25-cm2 superficial wounds were created over both third metacarpi of 6 horses. Forelimbs were randomly assigned to treatment (ESWT and bandage) or control (bandage only) groups. In treated limbs, each wound was treated with 625 shock wave pulses from an unfocused electrohydraulic shock wave generator. In control limbs, each wound received sham treatment. Wound appearance was recorded weekly as inflamed or healthy and scored for the amount of protruding granulation tissue. Standardized digital photographs were used to determine the area of neoepithelialization and absolute wound area. Biopsy was performed on 1 wound on each limb every week for 6 weeks to evaluate epithelialization, fibroplasia, neovascularization, and inflammation. Immunohistochemical staining for A smooth muscle actin was used to label myofibroblasts.
Results—Control wounds were 1.9 times as likely to appear inflamed, compared with treated wounds. Control wounds had significantly higher scores for exuberant granulation tissue. Treatment did not affect wound size or area of neoepithelialization. No significant difference was found for any of the histologic or immunohistochemical variables between groups.
Conclusions and Clinical Relevance—Treatment with ESWT did not accelerate healing of equine distal limb wounds, but treated wounds had less exuberant granulation tissue and appeared healthier than controls. Therefore, ESWT may be useful to prevent exuberant granulation tissue formation and chronic inflammation of such wounds, but further studies are necessary before recommending ESWT for clinical application.
Evaluation of a urine dipstick test for confirmation or exclusion of proteinuria in dogs
Authors: Zatelli Andrea , Paltrinieri Saverio , Nizi Francesca , Roura Xavier , Zini Eric 
Published: February 2010

Abstract
Objective—To evaluate a urine dipstick test as a possible replacement for urine protein-tocreatinine (UPC) ratio for identifying proteinuria in dogs.
Sample Population—507 urine samples from adult dogs.
Procedures—Urine dipstick, UPC ratio, specific gravity (USG), and sediment testing were performed on 507 samples. With UPC ratio as the reference criterion, diagnostic accuracy of the urine dipstick test was calculated for the entire data set and for urine samples grouped by USG (≤ 1.012 or > 1.012; < 1.030 or ≥ 1.030). A UPC ratio < 0.2 was used to indicate absence of proteinuria.
Results—The sensitivity of the urine dipstick test for detection of proteinuria was > 90% when 0 mg of protein/dL (a 0+ result) was used to indicate a negative test result, and the specificity ranged from 40% to 60%, depending on the USG. Sensitivity decreased to a range of 56% to 81% when 30 mg of protein/dL (a 1+ result) was used as the cutoff, depending on the USG, but the specificity increased to > 90%. The likelihood of correctly identifying nonproteinuric dogs was low when the USG was ≤ 1.012, particularly when samples with a 1+ result were considered negative.
Conclusions and Clinical Relevance—For dogs with a dipstick-test result of 1+ and USG ≤ 1.012, proteinuria should be assessed by use of the UPC ratio; dogs with a USG value > 1.012 are likely nonproteinuric. When used together, the urine dipstick test and USG measurement were reliable as a rapid alternative to UPC ratio determination in dogs in this study.
Efficacy of leflunomide for treatment of immune-mediated polyarthritis in dogs: 14 cases (2006–2008)
Authors: Colopy Sara A. , Baker Theresa A. , Muir Peter 
Published: February 2010

Abstract
Objective—To evaluate efficacy and adverse effects of leflunomide for the treatment of naturally occurring immune-mediated polyarthritis (IMPA) in dogs.
Design—Retrospective case series.
Animals—14 dogs with cytologically confirmed IMPA.
Procedures—Medical records were used to identify dogs with a diagnosis of IMPA that were treated with leflunomide. Signalment, radiographic findings, laboratory data, dosage of leflunomide, treatment duration, treatment response, and occurrence of adverse effects were determined from medical records.
Results—Mean ± SD initial dosage of leflunomide was 3.0 ± 0.5 mg/kg (1.4 ± 0.2 mg/lb) PO once daily. Treatment duration for the initial starting dosage ranged from 1 to 6 weeks. Of the 14 dogs treated with leflunomide, 8 had complete resolution of clinical signs of IMPA initially, 5 had partial response to treatment, and 1 had minimal response to treatment. Adverse effects from treatment with leflunomide were not observed during the treatment period.
Conclusions and Clinical Relevance—Oral administration of leflunomide was a safe and effective alternative to oral administration of corticosteroids for treatment of IMPA in dogs. On the basis of findings in this study, a starting dosage for leflunomide of 3 to 4 mg/kg (1.4 to 1.8 mg/lb) PO once daily for at least 6 weeks before making dose adjustments is recommended. Dose adjustments should be based on cytologic evaluation of synovial fluid and clinical signs of IMPA. Hematologic variables, serum biochemical analysis results, and clinical signs of IMPA should be monitored for evidence of adverse effects to treatment with leflunomide.
Management of mastitis and abscessation of mammary glands secondary to fibroadenomatous hyperplasia in a primiparturient cat
Authors: Burstyn Uri 
Published: February 2010

Abstract
Case Description—A 1-year-old sexually intact female domestic shorthair cat was evaluated because of an 8-week history of pronounced mammary gland hyperplasia that had progressed to mastitis and abscessation of the mammary glands since parturition 7 days earlier. The cat was anorectic, was febrile, and had signs of discomfort. Its kittens were weak and appeared to have difficulty nursing.
Clinical Findings—Physical examination revealed pyrexia, mastitis with abscessation in the 6 caudal mammary glands, skin ulceration over the nipples, and areas of skin necrosis over the abscessed mammary glands. A CBC revealed nonregenerative anemia and leukocytosis with a left shift (2.160 × 109 band cells/L) and toxic changes. Mastitis and incipient septicemia were considered the most likely causes. The history of mammary gland hyperplasia since the second week of pregnancy suggested a diagnosis of fibroadenomatous hyperplasia that predisposed the cat to subsequent mastitis.
Treatment and Outcome—Surgical drainage of the abscessed mammary glands, debridement of necrotic skin, and placement of a Penrose drain resulted in rapid improvement in clinical status. Broad-spectrum antimicrobial treatment (amoxicillin–clavulanic acid) was prescribed, and the cat was discharged from the hospital. Mastitis and fibroadenomatous mammary gland hyperplasia resolved rapidly afterward.
Clinical Relevance—Management of abscessed mammary glands through surgical drainage and drain placement is an option for treatment of cats with complications of fibroadenomatous hyperplasia. In the cat of this report, the treatment approach resulted in rapid resolution of mastitis, was less invasive than mastectomy, and avoided the potential complications of treatment with a progesterone-receptor antagonist.
Fecal egg counts after anthelmintic administration to aged horses and horses with pituitary pars intermedia dysfunction
Authors: McFarlane Dianne , Hale Grace M. , Johnson Eileen M. , Maxwell Lara K. 
Published: February 2010

Abstract
Objective—To determine effects of pituitary pars intermedia dysfunction (Cushing's disease) and age on fecal egg count and time to egg reappearance after anthelmintic treatment in horses residing in similar environments.
Design—Cross-sectional study.
Animals—29 healthy horses (4 to 35 years old) and 13 horses with PPID (13 to 33 years old).
Procedures—Fecal egg counts were performed by use of a modified Wisconsin flotation method at 2-week intervals before and after ivermectin treatment.
Results—Horses with PPID had higher fecal egg counts before and 8, 10, and 12 weeks after ivermectin treatment, compared with counts for site-matched healthy horses. There was no difference in the period for < 90% reduction in fecal egg counts between the 2 groups. Age did not affect fecal egg counts at any time point.
Conclusions and Clinical Relevance—For similar environmental conditions, horses with PPID were more likely to have higher fecal egg counts than were healthy horses. Therefore, horses with PPID may need to have a more aggressive parasite prevention program than do healthy horses. Age did not affect fecal egg counts or time to egg reappearance after anthelmintic treatment, which suggested age alone does not likely require special consideration when designing a parasite control program for adult horses.
Norm conditions for real-algebra isomorphisms between uniform algebras
Authors: Shindo Rumi 
Published: February 2010

Abstract
Let A and B be uniform algebras. Suppose that α ≠ 0 and A
1 ⊂ A. Let ρ, τ: A
1 → A and S, T: A
1 → B be mappings. Suppose that ρ(A
1), τ(A
1) and S(A
1), T(A
1) are closed under multiplications and contain expA and expB, respectively. If ‖S(f)T(g) − α‖∞ = ‖ρ(f)τ(g) − α‖∞ for all f, g ∈ A
1, S(e
1)−1 ∈ S(A
1) and S(e
1) ∈ T(A
1) for some e
1 ∈ A
1 with ρ(e
1) = 1, then there exists a real-algebra isomorphism $$
\tilde S
$$: A → B such that $$
\tilde S
$$(ρ(f)) = S(e
1)−1
S(f) for every f ∈ A
1. We also give some applications of this result.
On totally inert simple groups
Authors: Dixon Martyn , Evans Martin , Tortora Antonio 
Published: February 2010

Abstract
A subgroup H of a group G is inert if |H: H ∩ H
g| is finite for all g ∈ G and a group G is totally inert if every subgroup H of G is inert. We investigate the structure of minimal normal subgroups of totally inert groups and show that infinite locally graded simple groups cannot be totally inert.
Efficient representations of Green’s functions for some elliptic equations with piecewise-constant coefficients
Authors: Melnikov Yuri 
Published: February 2010

Abstract
Convenient for immediate computer implementation equivalents of Green’s functions are obtained for boundary-contact value problems posed for two-dimensional Laplace and Klein-Gordon equations on some regions filled in with piecewise homogeneous isotropic conductive materials. Dirichlet, Neumann and Robin conditions are allowed on the outer boundary of a simply-connected region, while conditions of ideal contact are assumed on interface lines. The objective in this study is to widen the range of effective applicability for the Green’s function version of the boundary integral equation method making the latter usable for equations with piecewise-constant coefficients.
Some properties of epimorphisms of Hilbert algebras
Authors: Buşneag Dumitru , Ghiţă Mircea 
Published: February 2010

Abstract
This paper represents a start in the study of epimorphisms in some categories of Hilbert algebras. Even if we give a complete characterization for such epimorphisms only for implication algebras, the following results will make possible the construction of some examples of epimorphisms which are not surjective functions. Also, we will show that the study of epimorphisms of Hilbert algebras is equivalent with the study of epimorphisms of Hertz algebras.
A procedure to compute prime filtration
Authors: Rauf Asia 
Published: February 2010

Abstract
Let K be a field, S = K[x
1, … x
n] be a polynomial ring in n variables over K and I ⊂ S be an ideal. We give a procedure to compute a prime filtration of S/I. We proceed as in the classical case by constructing an ascending chain of ideals of S starting from I and ending at S. The procedure of this paper is developed and has been implemented in the computer algebra system Singular.
Age and growth in a newly-established invasive population of topmouth gudgeon
Authors: Záhorská Eva , Kováč Vladmir , Katina Stanislav 
Published: February 2010

Abstract
Specimens of invasive topmouth gudgeon, Pseudorasbora parva, from Šúr Pond (Bratislava, Slovakia) were examined to assess age and growth, and to determine whether this recently-established invasive population employs a less specialized ontogenetic trajectory than the specialized form typical of native and/or long-time established populations. Samples were collected in October 2004 (n=143). Standard length (SL) ranged from 18.16 mm to 67.57 mm (mean 32.56 mm), and eviscerated body weight ranged from 0.10 to 5.02 g (mean 0.63 g). Scale caudal diameter ranged from 0.52 to 2.42 mm (mean 1.08 mm). SL at which the scales started to form was estimated to be 1.58 mm. The population was represented with 5 age groups, from 0+ to 4+. Relative to other populations for which comparable data are available the recently-established population of topmouth gudgeon was found to mature at smaller size and at a younger age compared to native and/or long-time established populations (all specimens bigger than 25.0 mm SL, and 94% of specimens from the age group I were already mature).
Far-infrared rays control prostate cancer cells in vitro and in vivo
Authors: Shima Hiroki , Yamamoto Shingo , Qiu Jun , Shincho Mayumi , Hirota Seiichi , Yoshikawa Yoshie , Yoshikawa Reigetsu , Hashimoto-Tamaoki Tomoko 
Published: February 2010

Abstract
We introduce a new effective method to control hormone-refractory prostate cancer cells using an activated rubber/resin form (RB), far-infrared ray (FIR) emitter, with or without sodium butyrate treatment (NaB). The growth of three human prostate cancer cell lines (Du145, PC-3 and LNCaP) was suppressed in vitro and vivo by FIR, and the cells were eradicated with FIR + 3 mM NaB. G1 arrest and apoptotic pathway proteins were induced by FIR with elevated expressions of apoptosis-related transcripts in cDNA microarray. RB reflects and radiates in the wavelengths of about 4 to 25 µm in the FIR that work to suppress the growth of human prostate cancer cells. Accordingly, this technique may be used as a new therapeutic treatment in hormone-refractory prostate cancer.
Phosphorylation is switch of L-type pyruvate kinase allostery
Authors: Faustova Ilona , Kuznetsov Aleksei , Juronen Erkki , Loog Mart , Järv Jaak 
Published: February 2010

Abstract
Among four pyruvate kinase isoenzymes, M1, M2, R and L, only M1 is considered as a nonallosteric enzyme. However, here we show that the non-phosphorylated L-type pyruvate kinase (L-PK) is also a non-allosteric enzyme with respect to its substrate phosphoenolpyruvate (PEP). The allosteric catalytic properties of L-PK are switched on through phosphorylation by cAMP-dependent protein kinase. The non-phosphorylated enzyme was produced by expressing the rat L-PK in E. coli, as the bacterium does not have mammalian-type protein kinases. The resulting tetrameric protein was phosphorylated with a stoichiometric ratio of one mole of phosphate per one L-PK monomer. Activity of the phosphorylated enzyme was allosterically regulated by PEP with the Hill coefficient n=2.5. It was observed that allostery was engaged by phosphorylation of the first subunit in the tetrameric enzyme, while further phosphorylation only modulated this effect. The discovered switching between non-allosteric and allosteric forms of L-PK and the possibility of modulating the allostery by phosphorylation are important for understanding of the interrelationship between allostery and the regulatory phosphorylation in general, and may have implication for further analysis of glycolysis regulation in the liver.