bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic Markers of Acute Suppurative Lung Disease
Authors: Fetlam D. L. , Chumachenko A. G. , Vyazmina M. D. , Moroz V. V. , Kuzovlev A. N. , Pisarev V. M. 
Published: April 2024

The mortality rate among patients with acute suppurative lung diseases (ASLD) in the ICU reaches 30%. Early, pathogenetically relevant biomarkers are needed to ensure personiﬁcation and better eﬃcacy of ASLD treatment. Numeric variations in the counts of immune system cells in patient’s blood can be viewed as such candidate biomarkers.The aim of the study. Identiﬁcation of potential markers predicting ASLD outcome after community-acquired pneumonia and COVID-19.Materials and methods. The study included 216 in-hospital patients aged 18-87 with ASLD after community-acquired pneumonia with (N=81) and without (N=135) COVID-19 history.Results. Patients survival after COVID-19 was linked to lymphocyte count on Day 1 of hospital stay (hazard ratio, HR=5.9 95%CI 0.9–37.4; P=0.0188, log-rank test). In patients who had not have COVID-19, a diﬀerence in survival was associated with lymphocyte (HR=2.9 95%CI 1.0–8.4; P=0.0184, log-rank test; N=135), and monocyte counts (HR=2.7 95% CI 0.8–9.5; P=0.0196, log-rank test) on Day 1 of hospital stay. Patients’ survival after COVID-19 infection depended on SII (systemic immune-inﬂammation index. HR=9.3 95%CI 1.7–49.8; P=0.0124, log-rank test; N=81, SIRI (systemic inﬂammatory response index, HR=7.2 95%CI 1.4–36.6; P=0.0339, log-rank test; N=81) and NLR (neutrophil-to-lymphocyte ratio, HR=9.6 95%CI 1.8–52.0; P=0.0108; log-rank test; N=81) values on Day 1 of hospital stay. In patients who did not have COVID-19 SII values had no inﬂuence on survival.Conclusion. The lymphocyte count makes it possible to predict outcomes of pleural empyema, regardless of patient’s history of COVID-19, i. e. a decrease in the lymphocyte count below 1.2×10⁹ in 1 L is associated with fatal outcome. Monocyte count carries prognostic information for cases of pleural empyema without previous COVID-19 infection. As for the relative indicators, SIRI, SII and NLR values measured on Day 1 in the hospital were predictors of ASLD outcome only in patients after COVID-19 infection, i. e., higher values were associated with increased risk of death, with NLR index being the most informative. Overall severity of illness above 10 scores by CIRS was associated with an unfavorable ASLD outcome, regardless of patient’s history of COVID-19.
Diagnosis and Intensive Care in Children’s Diabetic Acidosis: an Interdisciplinary Viewpoint
Authors: Aleksandrovich Yu. S. , Prometnoy D. V. , Petryaykina E. E. , Kiyaev A. V. , Peterkova V. A. , Kopylov V. V. , Muratov P. A. , Brezgin F. N. , Stepanenko S. M. , Lazukin A. V. , Pshenisnov K. V. , Alyokhina A. A. 
Published: April 2024

Diabetic ketoacidosis (DKA) is the main cause of death and disability in children with type I diabetes mellitus (T1DM). Children’s mortality from T1DM reaches 1% in developed countries and 13% in developing countries. The main cause of death in DKA is cerebral edema, clinical manifestations of which develop in 0.5–0.9% of children with DKA, while mortality riches 24%.Objective. Developing recommendations to prevent life-threatening complications of children with DKA using analysis of literature data and consolidated opinion of experts on the issues of intensive care in children with T1DM.Materials and methods. We analyzed and discussed studies in diagnosis and treatment of DKA in children with type 1 diabetes and 1200 literature sources since January 1970, published in Russian peer-reviewed scientiﬁc journals and international publications presented in the online repository Medline (Pubmed). The search for publications was carried out using the keywords: «children», «DKA», «DM1», «dehydration», «cerebral edema».Results. We considered issues of epidemiology, pathogenesis, clinical manifestations, diagnosis, intensive care for DKA, as well as clinical and diagnosis, treatment, prevention of cerebral edema issues in children. Limitations of the study were the small number of modern studies with a high level of evidence (randomized controlled trials, meta-analyses) over the past 5 years on DKA in children.Conclusion. Taking into account the national and international experience, joint recommendations on a consensus format were developed and formulated for the diagnosis of DKA, its leading complications and treatment recommendations for children with T1DM and DKA. Timely and accurate diagnosis of DKA, intensive therapy options based on proven therapeutic eﬃcacy, laboratory and clinical monitoring are warranted to interrupt the DKA pathogenesis, prevent the development of life-threatening conditions, and improve treatment outcomes for children with DKA.
Bond of textile-reinforced belite calcium sulfoaluminate cement mortar to concrete substrate
Authors: Cholostiakow Szymon , Ren Zhili , Skyrianou Ioanna , Koutas Lampros , Papakonstantinou Christos , Bescher Eric , Hanein Theodore 
Published: April 2024

AbstractThe fast aging of existing building stock requires effective and sustainable strengthening solutions. Textile-reinforced mortars (TRM) have already proved to be very effective as well as versatile retrofitting solutions for reinforced concrete and masonry structures. TRMs can enhance the load bearing capacity of reinforced concrete structures; however, current TRM systems are based on standard Portland cement-based binders, which largely contribute to global human-induced CO2 emissions. This work, for the first time, explores the use of belite calcium sulfoaluminate (BCSA) binder for carbon textile reinforcement through a cross-disciplinary study combining structural engineering and materials science. An experimental study was carried out on concrete block members with externally bonded strips of carbon textile-reinforced mortars, similar to a typical TRM retrofitting system for concrete beams. The textiles were embedded in an ordinary Portland cement-based (OPC) binder or in a BCSA-based binder to compare the bond behaviour to the concrete substrate. The tests revealed a superior bond between the BCSA mortar and the concrete, as well as outstanding adhesion to the textiles achieved using the BCSA binder, with performance levels largely surpassing those measured in their counterparts that used the OPC-based binder. Scanning Electron Microscopy, X-ray diffraction, and thermogravimetric analyses were used to understand this behaviour difference and it was concluded that the ettringite phase is responsible for the enhanced performance in the studied system. The results of this study suggest that BCSA binders have the potential to be a more effective and “greener” alternative to the standard binders based on Portland cement in TRM strengthening applications.
Long Non-coding RNA NEAT1, NOD-Like Receptor Family Protein 3 Inflammasome, and Acute Kidney Injury
Authors: Xue Rui , Yiu Wai Han , Chan Kam Wa , Lok Sarah W.Y. , Zou Yixin , Ma Jingyuan , Li Hongyu , Chan Loretta Y.Y. , Huang Xiao Ru , Lai Kar Neng , Lan Hui Yao , Tang Sydney C.W. 
Published: April 2024

Key Points

Long non-coding RNA (lncRNA) nuclear-enriched abundant transcript 1 (NEAT1) was upregulated in human and murine AKI. It returned to baseline after recovery in humans. Its knockdown preserved kidney function in animals.
In vitro, LPS upregulated NEAT1 by TLR4/NF-κB signaling and caused its translocation into the cytoplasm where it activated nucleotide oligomerization domain-like receptor family protein 3 by binding receptor of activated protein C kinase 1.

Background
AKI is common in hospitalized patients and is associated with high mortality. Inflammation plays a key role in the pathophysiology of AKI. Long non-coding RNAs (lncRNAs) are increasingly recognized as regulators of the inflammatory and immune response, but its role in AKI remains unclear.

Methods
We explored the role of lncRNA nuclear-enriched abundant transcript 1 (NEAT1) in (1) a cross-sectional and longitudinal cohort of AKI in humans, (2) three murine models of septic and aseptic AKI, and (3) cultured C1.1 mouse kidney tubular cells.

Results
In humans, hospitalized patients with AKI (N=66) demonstrated significantly higher lncRNA NEAT1 levels in urinary sediment cells and buffy coat versus control participants (N=152) from a primary care clinic; among six kidney transplant recipients, NEAT1 levels were the highest immediately after transplant surgery, followed by a prompt decline to normal levels in parallel with recovery of kidney function. In mice with AKI induced by sepsis (by LPS injection or cecal ligation and puncture) and renal ischemia-reperfusion, kidney tubular Neat1 was increased versus sham-operated mice. Knockdown of Neat1 in the kidney using short hairpin RNA preserved kidney function and suppressed overexpression of the AKI biomarker neutrophil gelatinase-associated lipocalin, leukocyte infiltration, and both intrarenal and systemic inflammatory cytokines IL-6, CCL-2, and IL-1β. In LPS-treated C1.1 cells, Neat1 was overexpressed by TLR4/NF-κB signaling and translocated from the cell nucleus into the cytoplasm where it promoted activation of nucleotide oligomerization domain-like receptor family protein 3 inflammasomes by binding with the scaffold protein receptor of activated protein C kinase 1. Silencing Neat1 ameliorated LPS-induced cell inflammation, whereas its overexpression upregulated IL-6 and CCL-2 expression even without LPS stimulation.

Conclusions
Our findings demonstrate a pathogenic role of NEAT1 induction in human and mice during AKI with alleviation of kidney injury in three experimental models of septic and aseptic AKI after knockdown of Neat1. LPS/TLR4-induced Neat1 overexpression in tubular epithelial cells increased the inflammatory response by binding with the scaffold protein, receptor of activated protein C kinase 1, to activate nucleotide oligomerization domain-like receptor family protein 3 inflammasomes.
Sero-prevalence and associated factors of hepatitis B virus infection among antenatal women at booking in a tertiary hospital
Authors: Esther Ijeoma Nonye-Enyidah , O. A. Altraide Basil , Solomon Enyidah Nonyenim , Awopola Jumbo I. , Mkpe Abbey 
Published: April 2024

Background: Hepatitis B virus (HBV) infection is a global public health problem affecting about two billion people worldwide. About 95% of the infection is acquired during the perinatal period. Though studies have been carried out on these in different parts of Nigeria, information regarding the prevalence and risk factors of HBV infection in antenatal clinic attendees in south-south Nigeria is scanty. We determined the sero-prevalence of hepatitis B surface antigen (HBsAg) and the associated factors among pregnant women in south-south, Nigeria.
Methods: A retrospective study carried out at the antenatal clinic of Rivers State university teaching hospital (RSUTH) between 1st January, 2015 and 31st December, 2020. At booking visit, 9,990 women were tested for HBsAg. Data was analyzed using the IBM SPSS version 23.0. P<0.05 was considered statistically significant.
Results: A total of 9,990 women were screened for HBV infection. Prevalence of HBV infection was 160 (1.6%). Age group 30-39 years had most HBV infection, 103 (64.4%). Most who were positive for HBsAg were married 158 (98.7%), traders 45 (28.1%), para 2-4, 65 (40.6%); in their second trimester, 78 (48.8%) and with tertiary level of education 111 (69.3%). Associated factors of HBV infection with statistically significant difference in proportion of HBsAg positives and negatives include gestational age, educational status, occupation, presence of HIV and VDRL.
Conclusions: Although the study reveals a low prevalence of HBV infection, continued routine screening for HBV infection is highly recommended.
A green approach to fingerprint enhancement: The potential of alternanthera dentata leaf powder
Authors: Srivastava Anshika , Verma Priyanka , Mukherjee Debhjit , Moza Bhavika , Saha Annesha , Ujjainia Poorvi , Dhondiyal Priyanka , Jain Pragati 
Published: April 2024

The field of forensic science necessitates the utilization of efficient techniques for the enhancement of latent fingerprints. Environmental issues are frequently raised when traditional approaches are employed in this context. This study introduces an innovative and ecologically responsible solution to this challenge in the form of Alternanthera Dentata (AD) leaf powder. AD is renowned for its striking and vivid deep purple leaves, and it unveils remarkable properties as a natural pH indicator. Its distinctive characteristic lies in the ability to undergo a vivid transformation from a rich crimson hue to a greenish-yellow tint, responding to alterations in pH levels, a phenomenon attributed to its anthocyanin content. The research demonstrates the versatility of AD leaf powder across diverse surfaces and its distinctive ability to indicate pH levels. The use of methanol for AD leaf powder extraction enhances the sensitivity of anthocyanins to pH fluctuations. The innovative approach not only proves the efficacy of AD leaf powder in enhancing fingerprint clarity but also emphasizes its eco-friendliness and cost-effectiveness. This study establishes Alternanthera Dentata (AD) leaf powder as a promising and sustainable solution for latent fingerprint enhancement in forensic science. The compatibility of AD with various surfaces, coupled with its natural pH indicator properties, positions it as a valuable resource. The incorporation of methanol further boosts sensitivity, highlighting the eco-friendly and cost-effective nature of this innovation. By embracing environmental sustainability principles, this research contributes to a greener and more responsible future for forensic science applications.
Rozwój sektora energii geotermalnej UE. Wykorzystanie potencjału pomp ciepła
Authors:   Instytut Nafty i Gazu – Państwowy Instytut Badawczy, Ciechanowska Maria 
Published: April 2024

W artykule przedstawiono stan wykorzystania energii geotermalnej w wybranych krajach UE w dwóch kierunkach: w procesie produkcji energii elektrycznej oraz do wytwarzania ciepła. W Polsce udział zainstalowanej mocy energii elektrycznej pochodzącej z energii geotermalnej wynosi zaledwie 0,27% mocy pozyskanej ze wszystkich źródeł OZE, zaś pozyskanie ciepła jest odpowiednio na poziomie 26,03%. Zwrócono uwagę, że zasoby geotermalne są użytkowane na różne sposoby i z różną intensywnością. W globalnym wykorzystaniu tej energii pierwsze miejsce zajmują geotermalne pompy ciepła. O ich wysokim potencjale wdrożeniowym decydują między innymi skala zastosowań i pozytywne opinie dotychczasowych użytkowników, dostępność rozwiązań technicznych w tym zakresie na całym rynku wewnętrznym UE, bogata oferta ze strony producentów pomp i instalatorów, często wzbogacona o możliwość współpracy z systemami fotowoltaicznymi czy magazynowaniem ciepła, a także możliwość uzyskania wsparcia finansowego inwestycji. Dopiero drugie miejsce w aspekcie wykorzystania energii geotermalnej zajmuje ciepłownictwo sieciowe. W tym przypadku koszty przedsięwzięcia niezwykle wzrastają w związku z koniecznością wykonania sieci otworów wiertniczych czy ze względu na ryzyko, jakie niesie ze sobą proces poszukiwań horyzontów geologicznych o pożądanych parametrach hydrotechnicznych i do tego w perspektywicznym interwale czasowym. Zaprezentowano wieloletni program rozwoju wykorzystania zasobów geotermalnych w Polsce na lata 2022–2040, a w pewnych obszarach do roku 2050, opublikowany przez Ministerstwo Klimatu i Środowiska. W ramach realizacji tego programu ustanowiono między innymi projekt pod nazwą „Udostępnienie wód termalnych w Polsce” – dotyczący odwiercenia 15 otworów dla poszukiwania i rozpoznania wyżej wymienionych wód w najbardziej perspektywicznych rejonach kraju pod względem możliwości eksploatacji źródeł geotermii głębokiej. Przedstawiono priorytetowe programy dotacyjne ukierunkowane na rozwój ogrzewnictwa indywidualnego, cieszące się w Polsce bardzo dużym zainteresowaniem. W programach takich jak „Mój Prąd”, „Moje Ciepło”, „Czyste Powietrze” istnieje możliwość uzyskania dofinansowania ze środków unijnych, między innymi do instalowanych pomp ciepła. Omówiono prace badawcze zrealizowane w Instytucie Nafty i Gazu – Państwowym Instytucie Badawczym, między innymi w zakresie możliwości wykorzystania sczerpanych złóż węglowodorów dla celów geotermalnych, biorąc pod uwagę możliwość zastosowania CO2 jako nośnika energii geotermalnej w procesie jego sekwestracji. Zaprezentowano zagadnienia tworzenia numerycznych modeli obiektów geotermalnych, doboru odpowiednich receptur płuczek wiertniczych, zaczynów cementowych czy płynów eksploatacyjnych przy uwzględnieniu podwyższonych temperatur i ciśnień.
Is it possible to anticipate complications after reconstructive surgery in patients with trophic disorders on the background of peripheral atherosclerosis in combination with diabetes mellitus?
Authors: Glushkov N. I. , Fadeeva E. A. , Puzdryak P. D. , Resnyanskaya E. D. , Kamenskaya A. E. , Gaifullin A. R. , Antonenko A. I. , Ivanov M. A. , Zhdanovich K. V. 
Published: April 2024

Background. Complications after revascularization in individuals with peripheral atherosclerosis and diabetes mellitus can lead to negative results of surgical interventions against the background of critical ischemia. Objective. The aim of the study was to identify predisposing circumstances for the development of certain complications after reconstruction in patients with critical ischemia and diabetes mellitus. Materials and methods. A prospective study of the results of reconstructive interventions was carried out in 78 patients with trophic disorders on the background of peripheral atherosclerosis and diabetes mellitus. Complications were detected in 25 cases (the main group), 53 patients had uncomplicated course of the postoperative period. The influence of concomitant diseases, peculiarities of surgical treatment on the occurrence of adverse outcomes was analyzed. Results. Manifestations of heart failure increase the likelihood of arterial thrombosis (p < 0.05). Signs of hepatic dysfunction increase the frequency of PE in the perioperative period (p < 0.05). Adequate correction of hypergly-cemia reduces the risk of stroke. Conclusion. The data on the circumstances predisposing for the development of complications make it possible to prepare more carefully for reconstruction against the background of critical ischemia in people with peripheral atherosclerosis and diabetes mellitus and minimize the number of adverse outcomes of intervention.
Typomorphic and Technological Characteristics of Quartz of the Mica-Pegmatite Formation on the Example of the Berkutinskaya Vein (South Urals)
Authors: Korekina M.A. , Savichev A.N. , Ankusheva N.N. , Pankrushina E.A. , Artemyev D.A. 
Published: April 2024

Abstract
—Understanding the origin of quartz veins of the mica-pegmatite formation is of great importance for deciphering the emplacement conditions for the of commercially prospective quartz targets. On the example of the Berkutinskaya vein, which is the typical metamorphogenic quartz deposit of the mica-pegmatite formation of the South Urals, we study the source and origin of the mineral-forming fluid, temperatures of quartz formation, the structure of intergranular boundaries, their morphometric feature, the fractal dimension of quartz grains and commercial characteristics of quartz grit. An integrated approach to the study of the typomorphic features of quartz allowed us to assess their impact on the technological characteristics of quartz concentrates. Comparison of data on the content of impurity elements in the Berkutinskaya quartz vein with quartz of the vein No. 175 of OOO Russian Quartz and IOTA-std (Sibelco) showed that the Berkutinskaya quartz vein is promising for commercial development.
Pangenome-based design of strain-specific primers allows the specific monitoring of engraftment in different habitats
Authors: Lebeer Sarah , Eilers Tom , Delanghe Lize , De Boeck Ilke , Rillaer Tim Van , Malderen Joke Van , Bakelants Sam , Beeck Wannes Van , Wittouck Stijn 
Published: April 2024

Abstract

Lactic acid bacteria (LAB), including many well-known beneficial bacteria, have seen a rise in the number of applications with specific strains across various areas, including live biotherapeutic products (LBPs). The most extensively researched strains belong to the Lactobacillaceae. Assessing the survival and persistence of specific strains in different niches is still an important challenge, while selective monitoring techniques are often lacking at strain level. Here, we show a robust pangenome-based approach for detecting singletons, which can be used to develop strain-specific primers. We developed selective and specific primers for six strains across different LAB species. The primers for the widely-used probiotic L. rhamnosus GG and L. plantarum WCFS1 were validated in in vivo studies and showed that these strains can persist in and on other habitats such as the human skin, upper respiratory tract and fermented vegetables. In conclusion, the selection of unique genes derived from the pangenome of a species resulted in a specific and sensitive method based on qPCR to detect and monitor strains in different habitats. This approach can be readily extended to other bacterial strains on other families for any type applications in research and industry.
Aedes aegypti mosquitoes (Diptera: Culicidae) can discriminate by blood sugar: new insights into comorbidity of dengue and diabetes
Authors: bursalı fatma 
Published: April 2024

Abstract

Purpose Aedes aegypti (L.,1762) is a primary vector of arboviral infections like dengue, yellow fever, Zika. Female mosquitoes are influenced by various physical and chemical cues from host when blood feeding, e.g., they find some individuals with certain blood types or certain conditions more attractive than others. This study determined whether Ae. aegypti shows a preference when offered blood from a patient with diabetes mellitus (DM), an endocrine disorder associated with abnormal glucose metabolism, compared to healthy blood from non-DMs
Methods In the dual feeding experiments, forty newly emerged female mosquitoes were provided with two blood feeding systems with blood from a non-diabetic (healthy) and diabetic patient using artificial feeders. Blood from 12 diabetic and 12 non-diabetic patients was matched by ABO blood type (e.g., diabetic type O blood was compared with non-diabetic type O blood). The number of mosquitoes that landed and fed from each membrane was counted every 2 minutes for thirty minutes.
Results Ae. aegypti species significantly preferred for blood from non-diabetic individuals (50-65% among the different blood type groups) compared to blood from diabetic individuals. Using multiplex allele-specific PCR it was also determined that, Ae. aegypti significantly preferred the O blood group regardless of blood sugar level compared to others.
Conclusion Ae. aegypti has less preference for diabetic blood to non-diabetics. Regardless people affected with this condition need to take preventive measures to reduce mosquito bites as they tend to have weaker immune systems and can experience more severe cases of dengue.