bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ubiquitin–proteasome system components are upregulated during intestinal regeneration
Authors: Pasten Consuelo , Ortiz‐Pineda Pablo A. , García‐Arrarás José E. 
Published: September 2011

AbstractThe ubiquitin proteasome system (UPS) is the main proteolytic system of cells. Recent evidence suggests that the UPS plays a regulatory role in regeneration processes. Here, we explore the possibility that the UPS is involved during intestinal regeneration of the sea cucumber Holothuria glaberrima. These organisms can regenerate most of their digestive tract following a process of evisceration. Initially, we identified components of H. glaberrima UPS, including sequences for Rpn10, β3, and ubiquitin‐RPL40. Predicted proteins from the mRNA sequences showed high degree of conservation that ranged from 60% (Rpn10) to 98% (Ub‐RPL40). Microarrays and RT‐PCR experiments showed that these genes were upregulated during intestinal regeneration. In addition, we demonstrated expression of alpha 20S proteasome subunits and ubiquitinated proteins during intestinal regeneration and detected them in the epithelium and connective tissue of the regenerating intestine. Finally, the intestinal regeneration was altered in animals treated with MG132, a proteasome inhibitor. These findings support our contention that proteasomes are playing an important role during intestinal regeneration. genesis, 50:350–365, 2012. © 2011 Wiley Periodicals, Inc.
A recombinase‐mediated cassette exchange‐derived cyan fluorescent protein reporter allele for Pdx1
Authors: Potter Leah A. , Choi Eunyoung , Hipkens Susan B. , Wright Christopher V.E. , Magnuson Mark A. 
Published: September 2011

AbstractFluorescent protein (FP) reporter alleles are useful both for identifying and purifying specific cell populations in the mouse. Here, we report the generation of mouse embryonic stem cells that contain a pancreatic and duodenal homeobox 1 (Pdx1) loxed cassette acceptor (Pdx1LCA) allele and the use of recombinase‐mediated cassette exchange to derive mice that contain a Pdx1CFP (Cerulean) reporter allele. Mice with this allele exhibited cyan fluorescence within the previously well‐characterized Pdx1 expression domain in posterior foregut endoderm. Immunolabeling showed that endogenous Pdx1 was coexpressed with CFP at all time points examined. Furthermore, fluorescence‐activated cell sorting was used to isolate CFP‐positive cells from E11.5 and E18.5 embryonic tissues using both 405 and 445 nm lasers, although the latter resulted in a nearly 50‐fold increase in emission intensity. The Pdx1CFP allele will enable the isolation of specific foregut endoderm and pancreatic cell populations, both alone and in combination with other FP reporter alleles. genesis 50:384–392, 2012. © 2011 Wiley Periodicals, Inc.
Properties of Metal Complexes of a New Dioxadiaza Macrocycle Containing a Dibenzofuran Unit and Acetate Pendant Arms
Authors: Mateus Pedro , Li Feng , Esteves Catarina V. , Delgado Rita , Brandão Paula , Félix Vítor 
Published: September 2011

AbstractA new dioxadiaza macrocycle containing a rigid dibenzofuran group (DBF) and bearing two acetate pendant arms, ac2[17](DBF)N2O2, has been synthesized in good yield by condensation of the parent macrocycle with potassium bromoacetate in basic aqueous solution. The protonation constants of ac2[17](DBF)N2O2 and the stability constants of its complexes with Mn2+, Ni2+, Cu2+, Zn2+, Cd2+ and Pb2+ ions were determined at 298.2 K in aqueous solutions and at ionic strength 0.10 mol dm–3 in NMe4NO3. The stability constants revealed that ac2[17](DBF)N2O2 has a higher affinity for larger metal ions, Cd2+ and Pb2+, showing a clear preference for cadmium. Spectroscopic data in solution and X‐ray crystal structure determinations revealed that the higher affinity for Cd2+ and Pb2+ is due to the better fit of these metal ions into the macrocyclic cavity, derived from its ring size and rigidity and the involvement of all the donor atoms of the ligand in the coordination to these metal centres. Considering the negative effect of these heavy metal ions on human health and the environment, this study represents an important step in the development of selective chelators for these two pollutants.
Azide‐Bridged Copper(II) and Manganese(II) Compounds with a Zwitterionic Tetrazolate Ligand: Structures and Magnetic Properties
Authors: Li Xiu‐Bing , Ma Yu , Zhang Xiu‐Mei , Zhang Jian‐Yong , Gao En‐Qing 
Published: September 2011

AbstractTwo azide‐bridged coordination polymers, [Cu(mptz)(N3)2] (1) and [Mn(mptz)(N3)2H2O] (2), (mptz = N‐methyl‐4‐pyridinium tetrazolate), were synthesized and crystallographically and magnetically characterized. Compound 1 is a neutral 1D coordination polymer in which the CuII ions, which are in a square pyramidal surrounding, are linked by double end‐on azide bridges in an alternating basal–basal and basal–apical fashion. Compound 2 exhibits a 3D MnII–azide coordination framework with the (10,3)‐b net topology, in which the double end‐on–azide‐bridged dimanganese units are interlinked by single end‐to‐end azide bridges. The magnetic analyses indicated that 1 behaves magnetically as a quasi‐dimer with an S = 1 ground state due to a strong ferromagnetic interaction through the basal–basal azide bridges and a very weak antiferromagnetic coupling through the basal–apical azide bridges. Compound 2 exhibits 3D antiferromagnetic behaviors with TN = 24.0 K, where the end‐to‐end and end‐on azide bridges mediate the antiferromagnetic and ferromagnetic interactions, respectively.
Combination of plasma microRNAs with serum CA19‐9 for early detection of pancreatic cancer
Authors: Liu Jianqiang , Gao Jun , Du Yiqi , Li Zhaoshen , Ren Yan , Gu Junjun , Wang Xiaowei , Gong Yanfang , Wang Weiwei , Kong Xiangyu 
Published: September 2011

AbstractThis study was performed to identify plasma microRNAs (miRNAs) as diagnostic biomarkers for pancreatic cancer (PCa) and to assess their supplementary role with serum CA19‐9 in early identification of tumors. Plasma RNAs were extracted from 140 PCa patients, 111 chronic pancreatitis (CP) patients and 68 normal controls, and the relative abundances of seven miRNAs (miR‐16, 21, 155, 181a, 181b, 196a and 210) were measured using real‐time PCR. Their diagnostic utility for PCa and correlation with clinical characteristics were analyzed. All seven miRNAs were significantly aberrantly upregulated in the PCa group compared with both the CP and normal groups, between which only four miRNAs (miR‐155, 181a, 181b and 196a) were significantly different. Logistic modeling proved that only miR‐16 and miR‐196a possessed an independent role in discriminating PCa from normal and CP. Furthermore, after including serum CA19‐9 in the logistic model, the combination of miR‐16, miR‐196a and CA19‐9 was more effective for discriminating PCa from non‐PCa (normal+CP) (AUC‐ROC, 0.979; sensitivity, 92.0%; specificity, 95.6%), and for discriminating PCa from CP (AUC‐ROC, 0.956; sensitivity, 88.4%; specificity, 96.3%) compared with the miRNA panel (miR‐16+miR‐196a) or CA19‐9 alone. Most significantly, the combination was effective at identification of tumors in Stage 1 (85.2%). In conclusion, plasma miRNAs were effective for distinguishing PCa from non‐PCa (normal+CP). The combination of miR‐16, miR‐196a and CA19‐9 was more effective for PCa diagnosis, especially in early tumor screening.
Glucose‐activated RUNX2 phosphorylation promotes endothelial cell proliferation and an angiogenic phenotype
Authors: Pierce Adam D. , Anglin Ian E. , Vitolo Michele I. , Mochin Maria T. , Underwood Karen F. , Goldblum Simeon E. , Kommineni Sravya , Passaniti Antonino 
Published: September 2011

AbstractThe runt‐related protein‐2 (RUNX2) is a DNA‐binding transcription factor that regulates bone formation, tumor cell metastasis, endothelial cell (EC) proliferation, and angiogenesis. RUNX2 DNA binding is glucose and cell cycle regulated. We propose that glucose may activate RUNX2 through changes in post‐translational phosphorylation that are cell cycle‐specific and will regulate EC function. Glucose increased cell cycle progression in EC through both G2/M and G1 phases with entry into S‐phase occurring only in subconfluent cells. In the absence of nutrients and growth factors (starvation), subconfluent EC were delayed in G1 when RUNX2 expression was reduced. RUNX2 phosphorylation, activation of DNA binding, and pRb phosphorylation were stimulated by glucose and were necessary to promote cell cycle progression. Glucose increased RUNX2 localization at focal subnuclear sites, which co‐incided with RUNX2 occupancy of the cyclin‐dependent kinase (cdk) inhibitor p21Cip1 promoter, a gene normally repressed by RUNX2. Mutation of the RUNX2 cdk phosphorylation site in the C‐terminal domain (S451A.RUNX2) reduced RUNX2 phosphorylation and DNA binding. Expression of this cdk site mutant in EC inhibited glucose‐stimulated differentiation (in vitro tube formation), monolayer wound healing, and proliferation. These results define a novel relationship between glucose‐activated RUNX2 phosphorylation, cell cycle progression, and EC differentiation. These data suggest that inhibition of RUNX2 expression or DNA binding may be a useful strategy to inhibit EC proliferation in tumor angiogenesis. J. Cell. Biochem. 113: 282–292, 2012. © 2011 Wiley Periodicals, Inc.
Watching osteogenesis: Life monitoring of osteogenic differentiation using an osteocalcin reporter
Authors: Born Anne‐Kathrin , Lischer Stefanie , Maniura‐Weber Katharina 
Published: September 2011

AbstractHuman bone marrow‐derived mesenchymal stem cells have the potential to differentiate into several cell types such as osteoblasts, chondrocytes, and adipocytes. When cultured under appropriate medium conditions stem cells can be directed toward the osteoblast lineage in vitro. Progression of osteogenic differentiation is accompanied by changes in the expression pattern of several marker proteins including bone‐specific alkaline phosphatase (bALP), collagen I (Col I), and osteocalcin (OC) and can be analyzed by well‐established methods like immunohistochemical staining and quantitative RT‐PCR. Furthermore, expression of fluorescent protein driven by an osteogenesis promoter facilitates online monitoring of proceeding osteogenic differentiation in transiently transfected human bone marrow‐derived cells. In the present study we established a new double reporter gene construct comprising OC promoter‐driven expression of green fluorescent protein and constitutive expression of red fluorescent protein‐tagged histone H2B for transient transfection of primary human bone cells (HBCs). Osteogenic differentiation of transiently transfected cells was visualized by fluorescence microscopy. Immunohistochemical analysis and RT‐PCR confirmed the progression into the osteo‐specific lineage of transfected cells. Transfection efficiency was determined by fluorescence‐activated cell sorting (FACS). J. Cell. Biochem. 113: 313–321, 2012. © 2011 Wiley Periodicals, Inc.
Therapeutic potential of hyaluronan oligosaccharides for bone metastasis of breast cancer
Authors: Urakawa Hiroshi , Nishida Yoshihiro , Knudson Warren , Knudson Cheryl B. , Arai Eisuke , Kozawa Eiji , Futamura Naohisa , Wasa Junji , Ishiguro Naoki 
Published: September 2011

AbstractHyaluronan (HA) oligosaccharides were reported to have suppressive effects on various malignant tumors via disruption of receptor HA interactions. However, no studies have focused on the effects of HA oligosaccharides on bone metastasis of breast cancer. In this study, we clarified the effective size of HA oligosaccharides required to inhibit cell growth in the highly invasive breast cancer cell line, MDA‐MB‐231 cells. Based on the results of cell growth assay, we subsequently analyzed the effects of HA tetrasaccharides, HA decasaccharides, and high molecular weight HA on the other breast cancer cell behaviors in vitro and breast cancer bone metastasis in vivo. HA decasaccharides significantly inhibited cell growth, motility, and invasion, whereas tetrasaccharides did not. HAS2 mRNA expression was altered after the treatment with both tetrasaccharides and decasaccharides. Phosphorylation of Akt was suppressed after 1 h treatment with HA decasaccharides, and the effect was partially reversed by anti‐CD44 monoclonal antibody. In vivo, local application of HA decasaccharides inhibited the expansion of osteolytic lesions in tibia on soft X‐rays using mouse bone metastasis model of breast cancer. Histological analysis revealed HA accumulation in bone metastatic lesions was perturbed by decasaccharides. These results suggest that HA oligosaccharides suppressed progression of bone metastasis in breast cancer via interruption of endogenous HA–CD44 interaction, and as such, can be a novel therapeutic candidate to limit bone metastasis of breast cancer. © 2011 Orthopaedic Research Society. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:662–672, 2012
Seminal Plasma Hypersensitivity Reactions: An Updated Review
Authors: Sublett J. Wesley , Bernstein Jonathan A. 
Published: September 2011

AbstractSeminal plasma hypersensitivity manifests as a spectrum of systemic and/or localized clinical symptoms after exposure to specific protein components in seminal fluid. The prevalence of this disease is largely unknown, but it is believed to affect up to 40,000 women in the United States. Although no definitive risk factors have been confirmed, women with systemic reactions are frequently atopic. Prostate‐specific antigen is believed to be the major allergen involved in the disorder, but other proteins are likely involved. Interestingly, up to 40%–50% of both systemic and localized seminal plasma hypersensitivity cases can occur after first‐time intercourse. Diagnosis is based on clinical history. The gold standard for diagnosing seminal plasma hypersensitivity is prevention of symptoms with the use of a condom. Patients with seminal plasma hypersensitivity demonstrate positive prick skin test and/or serum‐specific immunoglobulin E to whole seminal fluid or fractionated seminal plasma proteins. Treatment of seminal plasma hypersensitivity involves either avoidance with the use of condoms, intravaginal graded challenge using dilutions of whole seminal fluid, or subcutaneous desensitization to relevant fractionated seminal plasma proteins obtained from the woman's sexual partner. In most cases, treatment using one or more of the above approaches has been very successful. Infertility has not been demonstrated to be directly related to seminal plasma hypersensitivity, although women with the condition frequently have difficulty conceiving due to their inability to have unprotected sexual intercourse. Mt Sinai J Med 78:803–809, 2011. © 2011 Mount Sinai School of Medicine
Allergic Rhinitis
Authors: Mucci Tania , Govindaraj Satish , Tversky Jody 
Published: September 2011

AbstractAllergic rhinitis is a costly disease associated with significant morbidity. It impacts the quality of life of millions of individuals, particularly in industrialized nations, and it is on the rise. Lost productivity and total healthcare expenditure exceeds several billion dollars annually in the United States, with an estimate of >$6 billion spent on prescription medications alone. It is also associated with asthma and other atopic conditions, sinusitis, otitis media, and sleep apnea. Primary care physicians should be well adept at recognizing and initiating empiric first‐line therapy for chronic rhinitis. Allergen avoidance, topical nasal steroids, and antihistamines may be sufficient for some patients. In most cases, referral to a board‐certified allergy specialist for skin testing and targeted management is indicated. It is essential to make sure that patients abstain from using antihistamines at least 1 week prior to reporting to the allergist for skin testing in order to avoid false‐negative results. Traditional subcutaneous allergen immunotherapy, when performed by an experienced allergist, affords relief in >75% of cases. The growing armament of treatment options for refractory allergic rhinitis includes oral and sublingual immunotherapy, recombinant allergens, conjugated DNA vaccines, and anti–immunoglobulin E monoclonal antibody. Mt Sinai J Med 78:634–644, 2011. © 2011 Mount Sinai School of Medicine
Urticaria and Angioedema
Authors: Axelrod Sara , Davis‐Lorton Mark 
Published: September 2011

AbstractUrticaria and angioedema are common disorders that can severely impair the quality of a patient's life and can be extremely difficult to treat. Symptoms can persist for years to decades. The causes of urticaria and angioedema are varied and may be immunologic, nonimmunologic, or idiopathic. This article reviews the literature and provides primary care physicians with up‐to‐date information of the epidemiology, basic pathophysiology, diagnosis, and management of this common and often debilitating condition. Additionally, clinical manifestations of acute and chronic urticaria, hereditary and acquired angioedema, as well as the physical urticarias will be discussed. Mt Sinai J Med 78:784–802, 2011. © 2011 Mount Sinai School of Medicine
Relationship of sense of coherence to stressful events, coping strategies, health status, and quality of life in women with breast cancer
Authors: Sarenmalm Elisabeth Kenne , Browall Maria , Persson L.‐O. , Fall‐Dickson J. , Gaston‐Johansson Fanny 
Published: September 2011

AbstractObjectiveTo test the hypothesis that Antonovsky's concept of sense of coherence (SOC) predicts stressful events, coping strategies, health status, and quality of life (QoL) in a cohort of postmenopausal women (n = 131) with newly diagnosed primary or recurrent breast cancer.MethodsRegression analyses of longitudinal data at baseline through 6 months following breast cancer diagnosis examined the relationships between SOC (13‐item version), daily assessment of coping with stressful events, health status, and QoL (EORTC QLQ‐30).ResultsThe findings support Antonovsky's concept of SOC. Women with strong SOC reported fewer stressful events and more days without stressful events. They used more coping strategies and more frequently used distraction, situation redefinition, direct action, and relaxation, but seldom religion, to cope with stressful events, and reported better health status and QoL. Women with weak SOC experienced more distress and used fewer coping strategies, and they more frequently used coping strategies such as catharsis and seeking social and spiritual support, but seldom acceptance of the situation. They reported worse health status and QoL, regardless of disease stage or treatment. The relationships between SOC and health status and QoL were linear.ConclusionsSense of coherence significantly predicts distress, number and type of coping strategies such as direct action and relaxation, health status, and QoL in women with breast cancer. Our data suggest that the SOC scale may be a useful screening tool to identify individuals particularly vulnerable to distress and unable to cope adequately. Assessing SOC strength may assist health care providers to provide individualized patient interventions. Copyright © 2011 John Wiley & Sons, Ltd.
2‐Lithiumamide‐2‐fluoro‐1,3‐diaza‐2,4‐disilacyclobutanes – Syntheses, Reactions, Structures
Authors: Kanaan Fatme , Schulzke Carola , Strüber Katharina , Klingebiel Uwe 
Published: September 2011

AbstractThe cyclodisilazane Me2Si(NCMe3)2SiF2 (1) reacts with lithium salts of ammonia (2), hydrazine (3) and amines (4–10) to give the stable compounds Me2Si(NCMe3)2SiFNHR (R = H (2), NH2 (3) CMe3 (4), C10H15 (5), 3,5‐C6H3Me2 (6), 2,4,6‐C6H2Me3 (7), 2,6‐C6H3(CHMe2)2 (8), C6F5 (9), Si(CMe3)2Ph (10)). Compounds 2–10 show no condensation reactions with formation of NH3, N2H4, or amines. Dilithiummethylenediamide reacts with 1 to give the ethylenediamine coupled ring system 11 or spirocyclic compound 12. Compounds 2, 3, 4 form the spirocyclic systems 13–15 in reactions with n‐C4H9Li. A partly hydrolysed lithium salt (16) was isolated as tetramer containing a central Li6O octahedron. The lithium salts of 5 (17), 9 (19) and 10 (20) were isolated as monomers (17, 19) or dimer, respectively, via an (LiN)‐four‐membered ring (20). Crystal structures of 3, 9, 13–17, 19 and 20 are reported.
Effects of Time on Ultrastructural Integrity of Parathyroid Tissue Before Cryopreservation
Authors: Barreira Carlos Eduardo Santa Ritta , Cernea Cláudio Roberto , Brandão Lenine Garcia , Custódio Melani Ribeiro , Caldini Elia Tamaso Espin Garcia , de Menezes Montenegro Fábio Luiz 
Published: September 2011

AbstractBackgroundCryopreservation of parathyroid tissue is used in the surgical treatment of secondary hyperparathyroidism. After surgical resection, the tissue is temporarily maintained in a cell culture solution until it arrives at the specialized laboratory where the cryopreservation process will take place. The present study evaluates the time that the human hyperplastic parathyroid gland tissue can wait before cryopreservation, based on parathyroid cell ultrastructural integrity.MethodsThis prospective study included 11 patients who underwent total parathyroidectomy with heterotopic autotransplantation and cryopreservation of parathyroid tissue fragments. Part of the tissue was kept in cell culture solution at 4°C. Five time periods between 2 and 24 h were defined, and parathyroid fragments were kept in the solution for that length of time. At the end of each period, the fragments were removed from the transport solution, fixed, and prepared for ultrathin sections.ResultsOf the 11 cases studied, 10 showed ultrastructural findings consistent with cellular viability in tissue fragments that remained in the transport solution up to 12 h. Electron microscopy revealed that cell adhesion and the integrity of plasma membranes, nuclei, and mitochondria were preserved in one case for up to 24 h. Changes in mitochondrial structure represented the most constant ultrastructural damage seen in the cases studied, in addition to the presence of edema and cell vacuoles.ConclusionsAnalysis of the ultrastructure of hyperplastic parathyroid gland tissue showed that ultrastructural integrity was in most cases properly maintained in fragments stored up to 12 h in a cell culture solution at 4°C.
Predictive Value of Baseline Neutrophil/Lymphocyte Ratio for T4 Disease in Wall‐Penetrating Gastric Cancer
Authors: Aizawa Masaki , Gotohda Naoto , Takahashi Shinichiro , Konishi Masaru , Kinoshita Taira 
Published: September 2011

AbstractBackgroundMultimodality therapy has been used in the management of gastric cancer associated with locoregional spread. However, the accurate clinical staging still remains to be established. The neutrophil/lymphocyte ratio (NLR) in the peripheral blood is reported to be an easily assessable prognostic factor in cancer patients. We evaluated the predictive significance of the NLR and other serological parameters in patients with wall‐penetrating gastric cancer.MethodsTwo hundred sixty‐two patients who underwent gastric cancer surgery between 2002 and 2005 were identified retrospectively. Wall penetration was defined as wall invasion deeper than the muscularis propria (≥T2). Blood data were collected from routinely performed blood examinations before treatment and were analyzed with respect to T stage, nodal status, and histological features. A high NLR was defined as less than 3.2 based on ROC curve analysis, and the predictive value of a high NLR for T4 cancer was evaluated.ResultsElevated levels of NLR (P = 0.004) and C‐reactive protein (CRP) (P = 0.017) and the decrease in lymphocyte count (Lym, P = 0.032) and serum hemoglobin (Hb, P < 0.001) were correlated with the T stage, but there was no meaningful correlation with either positive nodal status or histological differentiation. With respect to the predictive value for stage T4, an elevated NLR (OR = 2.206, 95% CI = 1.187–4.100; P = 0.012), decrease of Hb (OR = 1.875, 95% CI = 1.005–3.500; P = 0.048), and poorly differentiated histology (OR = 3.134, 95% CI = 1.593–6.167; P = 0.001) were identified as independent predictive factors.ConclusionOur findings suggest that the preoperative values of the NLR may be reliable for predicting T4 disease.