bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hematopoietic stem cell transplantation in severe congenital neutropenia
Authors: Carlsson G. , Winiarski J. , Ljungman P. , Ringdén O. , Mattsson J. , Nordenskjöld M. , Touw I. , Henter J.‐I. , Palmblad J. , Fadeel B. , Hägglund H. 
Published: November 2010

AbstractBackgroundSevere congenital neutropenia (SCN) is an immunodeficiency characterized by disturbed myelopoiesis and an absolute neutrophil count (ANC) <0.5 × 109/L. SCN is also a premalignant condition; a significant proportion of patients develop myelodysplastic syndrome or leukemia (MDS/L). Allogeneic hematopoietic stem cell transplantation (HSCT) is the only curative treatment for SCN.ProcedureSince 2004, eight HSCT have been performed in seven patients at our center. The indications were transformation to MDS/L (n = 2), granulocyte colony‐stimulating factor receptor (CSF3R) mutation(s) (n = 2), granulocyte colony‐stimulating factor (G‐CSF) resistance (n = 2), and at the patient's own request (n = 1).ResultsThe mean age at transplantation was 13 years (2.8–28 years) (mean follow‐up 32 months, range 21–60). Three patients harbored ELANE mutations, three HAX1 mutations, and in one patient no causative mutation was identified. Two of the ELANE mutations were novel mutations. Three patients initially received myeloablative conditioning and four had reduced intensity conditioning (RIC). Three grafts were from HLA‐identical siblings, three from matched unrelated donors and two were cord blood units. Engraftment occurred in all patients. Two of seven (29%) patients died; both had MDS/L and both were among the three that underwent myeloablative conditioning. One patient has chronic GVHD 2 years post‐transplant.ConclusionsThe role of HSCT should be explored further in patients with SCN. In particular, the influence of the conditioning regime needs to be evaluated in a larger cohort of patients. Pediatr Blood Cancer 2011;56:444–451. © 2010 Wiley‐Liss, Inc.
Molecular determination of RHD zygosity:predicting risk of hemolytic disease of the fetus and newborn related to anti‐D
Authors: Pirelli Kevin J. , Pietz Bradley C. , Johnson Susan T. , Pinder Holly L. , Bellissimo Daniel B. 
Published: November 2010

AbstractObjectiveDevelopment of an accurate molecular method for paternal RHD zygosity to predict risk to a fetus for hemolytic disease of the fetus and newborn (HDFN) related to anti‐D.MethodsQuantitative fluorescence polymerase chain reaction (QF‐PCR) was used to detect RHD exons 5 and 7, using RHCE exon 7 as an internal control. The genotype and zygosity were determined from the peak area ratios of RHD exon 5 or 7 to RHCE exon 7. We tested 25 Caucasian and 25 African American (AA) samples whose zygosity was predicted from the Rh phenotype and an alternate molecular method. In addition, we tested 71 paternal samples from prenatal cases where fetal testing was performed.ResultsRHD/RHCE ratios clearly distinguished the RHD/D and RHD/d genotypes. RHD variants were recognized when RHD exon 5 copy number was discordant with exon 7. The molecular assay identified eight cases where the phenotype incorrectly assigned zygosity and we observed three false‐negatives in the hybrid Rhesus box assay. The prenatal results were consistent with the zygosity determined for the paternal samples in our study.ConclusionsThis QF‐PCR method accurately determines RHD zygosity in Caucasians and AAs and will help predict the risk that a fetus will inherit RHD. Copyright © 2010 John Wiley & Sons, Ltd.
Periconceptional cytomegalovirus infection: pregnancy outcome and rate of vertical transmission
Authors: Hadar Eran , Yogev Yariv , Melamed Nir , Chen Rony , Amir Jacob , Pardo Josef 
Published: November 2010

AbstractObjectiveTo evaluate pregnancy outcome and rate of vertical transmission in primary maternal periconceptional cytomegalovirus (CMV) infection.MethodsAll women serologically diagnosed with primary periconceptional CMV infection between 1999 and 2008 were included. Periconceptional infection was defined as primary maternal CMV infection occurring within 4 weeks prior to the last reported menstrual period and up to 3 weeks following the expected date of the missed menstrual period. Intrauterine infection was verified by PCR and shell vial culture of amniotic fluid at 22–24 weeks or neonatal urine culture within 48 h of birth.ResultsOf the 59 patients studied, 43 (73%) underwent diagnostic amniocentesis. Eleven of the 43 patients (25.5%) were positive for CMV contamination. Ten of the 11 patients (90%) elected to terminate pregnancy. Twelve women (20.3%) declined amniocentesis: of these 2 elected to undergo a first‐trimester termination of pregnancy and 10 gave birth to a live‐born infant. Six of the ten neonates were negative for CMV and two tested positive for urinary CMV; all eight were healthy on long‐term follow‐up, and two were lost to follow‐up. The remaining four women had a spontaneous first‐trimester abortion.ConclusionThe risk of transmission of periconceptional CMV infection is lower than previously reported. These data should be borne in mind when counseling affected couples. Copyright © 2010 John Wiley & Sons, Ltd.
Linkage of mother–baby pairs in the German Pharmacoepidemiological Research Database
Authors: Garbe Edeltraut , Suling Marc , Kloss Sebastian , Lindemann Christina , Schmid Ulrike 
Published: November 2010

AbstractPurposeAdministrative healthcare databases are increasingly being used to investigate potential drug risks in pregnancy. Our study aimed to develop an algorithm for linkage of mother–baby pairs (MBPs) in the German Pharmacoepidemiological Research Database (GePaRD) as a prerequisite for such studies.MethodsGePaRD contains sociodemographic data, drug dispensations, ambulatory, and hospital information on more than 14 million insurants from four German statutory health insurances (SHIs) covering all regions in Germany. Linkage was based on co‐insurance information of the newborn with the potential mother (direct linkage) or of both potential mother and newborn with the potential father (indirect linkage). Linkage is not possible if the baby is co‐insured with the father and the mother is self‐insured. Further information on birth or childbed was used to validate the potential mother as true mother in MBP. Descriptive comparisons between linked and unlinked mothers were conducted.ResultsOf 323 993 newborns identified between 2004 and 2006, 250 355 (77.3%) could be linked in MBP. Of those, 189 702 (75.8%) MBP were based on direct linkage. Mean age was similar in linked (31.1 years, standard deviation (SD = 5.4) and unlinked (31.8 years, SD = 5.5) mothers as was the proportion of caesarean sections in both groups (28.9% vs. 29.3%).ConclusionsThe developed algorithm permits linkage of a great number of newborns with their mothers and creates a potential data source for investigation of drug risks in pregnancy. Further validation studies are needed also including information on pregnancies not resulting in live births. Copyright © 2010 John Wiley & Sons, Ltd.
Demographic, medical, and behavioral characteristics associated with over the counter non‐steroidal anti‐inflammatory drug use in a population‐based cohort: results from the Multi‐Ethnic Study of Atherosclerosis
Authors: Delaney Joseph A.C. , Biggs Mary L. , Kronmal Richard A. , Psaty Bruce M. 
Published: November 2010

AbstractBackgroundThree types of non‐steroidal anti‐inflammatory drugs (NSAIDs) can be obtained both over the counter (OTC) and by prescription in the United States. OTC NSAID use is not recorded in prescription claims databases; this might lead to differential misclassification of NSAID exposure status in studies that use computerized pharmacy databases to study NSAID use.ObjectiveTo evaluate characteristics of OTC versus prescription NSAID users.MethodsThis analysis is set within the Multi‐Ethnic Study of Atherosclerosis (MESA) study; a prospective cohort study of 6814 adults from four ethnic groups (European descent, Asian, African‐American, and Hispanic) with a mean age of 62 years. The cohort was restricted to those who initiated NSAID use (aspirin, ibuprofen, or naproxen) during follow‐up. We compared information about age, sex, ethnicity, body mass index, smoking, diabetes, medication use, education, income, health insurance status, and exercise between groups.ResultsOTC NSAID use was prevalent at baseline (25% aspirin, 9% ibuprofen, and 2% naproxen). Compared to prescribed NSAID use, OTC NSAID use was lower for users of non‐European descent for all classes: aspirin (p < 0.0001), ibuprofen (p < 0.0001), and naproxen (p = 0.0094). For aspirin, differences were seen for male gender (relative risk (RR): 0.92; 95%confidence interval (CI): 0.86–0.98), use of lipid lowering drugs (RR: 0.88; 95%CI: 0.80–0.96), low income (RR: 0.89; 95%CI: 0.81–0.97), and participants one standard deviation above average in intentional exercise (RR: 1.03; 95%CI: 1.01–1.05).ConclusionsOTC NSAID use is prevalent in an older multi‐ethnic population and OTC users differ from prescription NSAID users. Caution should be exercised when using prescribed NSAIDs as a proxy for NSAID use. Copyright © 2010 John Wiley & Sons, Ltd.
Did the removal of the indication of nocturnal enuresis for intranasal desmopressin change prescribing practice?
Authors: Hoffmann Falk , Glaeske Gerd , Steuber Christian 
Published: November 2010

AbstractPurposeThe indication for the treatment of primary nocturnal enuresis was removed from all intranasal preparations of desmopressin in May 2007. Objective of this study was to examine whether and how fast this regulatory decision changed prescribing in affected children.MethodsWe analyzed claims data of the Gmünder ErsatzKasse (GEK) over the years 2004–2008. All children and adolescents aged 0–18 years who received at least one out‐patient diagnosis of urinary incontinence in the corresponding years were included. Our outcome of interest was the proportion of oral desmopressin and its change over time.ResultsA total of 6308 to 7207 children with a mean age of about 8 years were included annually (62–63% were male) and 14 746 packages of desmopressin were analysed (49.9% intranasal; 50.1% oral; 0.01% parenteral preparations). The proportion of patients using desmopressin decreased slightly from 13.9% in 2004 to 12.6% in 2008 (p for trend = 0.0131). Between January 2004 (39.1%) and December 2006 (41.3%), the proportion of oral forms was nearly constant and doubled after that within a few months to about 80%.ConclusionsImmediately after the removal of the indication for intranasal desmopressin, an increased prescribing of tablet forms in affected children was found in Germany. Copyright © 2010 John Wiley & Sons, Ltd.
Transport properties of p‐type compensated silicon at room temperature
Authors: Rougieux F.E. , Macdonald D. , Cuevas A. 
Published: November 2010

AbstractDeliberate dopant compensation has proven to be an effective way to control ingot resistivity, although the impact of compensation on carrier recombination and mobilities remains under investigation. This paper summarizes recent findings regarding the carrier transport properties of compensated silicon. The capacity of common mobility models to describe compensated silicon is reviewed and compared to experimental data. The observed reduction of both majority and minority carrier mobility due to dopant compensation is described in terms of the underlying scattering mechanisms. The related problem of conversion between resistivity and dopant density in compensated silicon is discussed, and published values of the Hall Factor in compensated silicon are reviewed. Copyright © 2010 John Wiley & Sons, Ltd.
Epitaxy‐free monocrystalline silicon thin film: first steps beyond proof‐of‐concept solar cells
Authors: Depauw V. , Qiu Y. , Van Nieuwenhuysen K. , Gordon I. , Poortmans J. 
Published: November 2010

AbstractThe “Epifree” process involves the lift‐off of a high‐quality monocrystalline film formed by reorganization upon annealing of cylindrical macropore arrays in silicon, and can thus provide high‐quality silicon films without resorting to costly epitaxy. The challenge of this new process lies in etching controlled and regular pores in silicon in a cost‐efficient way, and in developing a process compatible with the difficulty of handling a micron‐thin material. Proof‐of‐concept cells have previously been achieved and this paper presents the latest progress, with a first development of thicker films and the inclusion of rear‐side passivation. The energy‐conversion efficiency of 1‐µm‐thin Epifree cells was improved from 2.6 to 4.1% by depositing a stack of amorphous silicon (a‐Si) layers as rear‐side passivation. The increase in Voc was, however, limited and bound to a drop in Jsc. The choice of a‐Si was revealed to be unsuitable because of the thinness of the film and the presence of a full aluminum rear contact. The thinness of the film leads to a decrease in rear‐side reflectivity by the a‐Si absorption, and the aluminum, although not leading to crystallization, partly migrates inside the a‐Si stack upon anodic bonding as shown by TEM. These factors indicate that an alternative surface passivation should be developed. In parallel to process developments, the material was thickened by modifying the macropore array dimensions, leading to a 2.4‐µm‐thick material over 1 cm × 1 cm areas. The efficiency of the next cells is expected to increase with this thicker material. Copyright © 2010 John Wiley & Sons, Ltd.
High efficiency CIGS based solar cells with electrodeposited ZnO:Cl as transparent conducting oxide front contact
Authors: Rousset Jean , Saucedo Edgardo , Herz Konrad , Lincot Daniel 
Published: November 2010

AbstractIn this paper high efficiency (up to 15.8%) Mo/Cu(In,Ga)Se2/CdS/i‐ZnO solar cells terminated with an electrodeposited ZnO:Cl window layer are reported. The optoelectronic properties of these cells are comparable to reference cells terminated with standard sputtered ZnO:Al. The use of an electrochemical step, a low cost and easily up‐scalable method, in the fabrication process of this type of solar cell, opens the possibility to lower their production price and make them more competitive. The optimization involves a soft annealing treatment (T ≤ 150°C) under atmospheric conditions after the electrodeposition which improves the transparency of the ZnO:Cl layers in the infrared region, the short circuit current and the fill factor of the I–V curve. These changes are related to the increase of one order of magnitude (up to 5 × 102 Ω−1·cm−1) of the lateral conductivity of the electrochemical layer due to an improvement of the free carrier mobility. Copyright © 2010 John Wiley & Sons, Ltd.
Novel organotin‐containing shell‐cross‐linked knedel and core‐cross‐linked knedel: Synthesis and application in catalysis
Authors: Feng Gang , Jia Yongguang , Liu Lingyan , Chang Weixing , Li Jing 
Published: November 2010

AbstractA series of novel organotin‐containing core‐cross‐linked knedels and shell‐cross‐linked knedels were first synthesized facilely from poly(styrene)‐b‐poly(acrylate acid) nanoparticles in different selective solvents [Tetrahydrofuran (THF)/H2O or THF/n‐octane] by using organotin compound 1,3‐dichloro‐tetra‐n‐butyl‐distannoxane as a new cross‐linker. The formation of the 1‐chloro‐3‐carboxylato‐tetra‐n‐butyl‐distannoxane layer in our cross‐linking reaction was supported by Fourier transform infrared (FT‐IR) and inductive coupled plasma emission spectrometer (ICP) analysis of the resulting shell‐cross‐linked knedels and core‐cross‐linked knedels. Transmission electron microscopy (TEM) study showed the spherical morphology and the size of the core‐cross‐linked knedels and shell‐cross‐linked knedel. Especially, the layer structure of the core‐cross‐linked knedels was clearly displayed in TEM image. The increase of extent of cross‐linking lead to the increasing of diameter for the shell‐cross‐linked knedels, whereas there was no significant effect on the core‐cross‐linked knedels. Dynamic light scattering (DLS) measurements gave hydrodynamic diameters of the core‐cross‐linked knedels that were in agreement with the TEM diameters. Moreover, the wall thickness of the shell layer of the core‐cross‐linked knedels could be easily modified by varying the block copolymer composition. Notably, the organotin‐containing core‐cross‐linked knedel exhibited highly efficient catalytic activity for the aqueous esterification reaction under nearly neutral conditions. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2010
The mechanism of papain inhibition by peptidyl aldehydes
Authors: Shokhen Michael , Khazanov Netaly , Albeck Amnon 
Published: November 2010

AbstractVarious mechanisms for the reversible formation of a covalent tetrahedral complex (TC) between papain and peptidyl aldehyde inhibitors were simulated by DFT calculations, applying the quantum mechanical/self consistent reaction field (virtual solvent) [QM/SCRF(VS)] approach. Only one mechanism correlates with the experimental kinetic data. The His–Cys catalytic diad is in an N/SH protonation state in the noncovalent papain–aldehyde Michaelis complex. His159 functions as a general base catalyst, abstracting a proton from the Cys25, whereas the activated thiolate synchronously attacks the inhibitor's carbonyl group. The final product of papain inhibition is the protonated neutral form of the hemithioacetal TC(OH), in agreement with experimental data. The predicted activation barrier g = 5.2 kcal mol−1 is close to the experimental value of 6.9 kcal mol−1. An interpretation of the experimentally observed slow binding effect for peptidyl aldehyde inhibitors is presented. The calculated g is much lower than the rate determining activation barrier of hemithioacetal formation in water, g, in agreement with the concept that the preorganized electrostatic environment in the enzyme active site is the driving force of enzyme catalysis. We have rationalized the origin of the acidic and basic pKa's on the k2/KS versus pH bell‐shaped profile of papain inhibition by peptidyl aldehydes. Proteins 2011. © 2010 Wiley‐Liss, Inc.
Fingerprint‐based structure retrieval using electron density
Authors: Yin Shuangye , Dokholyan Nikolay V. 
Published: November 2010

AbstractWe present a computational approach that can quickly search a large protein structural database to identify structures that fit a given electron density, such as determined by cryo‐electron microscopy. We use geometric invariants (fingerprints) constructed using 3D Zernike moments to describe the electron density, and reduce the problem of fitting of the structure to the electron density to simple fingerprint comparison. Using this approach, we are able to screen the entire Protein Data Bank and identify structures that fit two experimental electron densities determined by cryo‐electron microscopy. Proteins 2011. © 2010 Wiley‐Liss, Inc.
Flexible Replication Technique for High‐Aspect‐Ratio Nanostructures
Authors: Szeghalmi Adriana , Sklarek Kornelia , Helgert Michael , Brunner Robert , Erfurth Wilfried , Gosele Ulrich , Knez Mato 
Published: November 2010

AbstractA flexible, nondestructive, and cost‐effective replication technique for nanostructures is presented. The advantages of the process are: 1) it allows for tailoring structural parameters of the replica (e.g., line width) nearly independent of the structural geometry of the master; 2) it allows for replication of high‐aspect‐ratio structures also in polymer materials from solution (especially noncurable polymers) such as polystyrene and polymethylmethacrylate; 3) it includes an easy separation process, thus preserving the master for repeated use. Linear grating replicas with line widths ranging from 88 to 300 nm are obtained using a single nanostructured master. Nanofibers and complex nanopatterned replicas are achievable. The presented technique and its flexibility show that atomic layer deposition is a unique tool for the preparation of high‐efficiency polarizer diffractive optics, photonics, electronics, and catalysts.
A Biodegradable and Biocompatible Regular Nanopattern for Large‐Scale Selective Cell Growth
Authors: Csaderova Lucia , Martines Elena , Seunarine Krishna , Gadegaard Nikolaj , Wilkinson Chris D. W. , Riehle Mathis O. 
Published: November 2010

AbstractA biodegradable substrate with a regular array of nanopillars fabricated by electron‐beam lithography and hot embossing is used to address the mechanisms of nanotopographical control of cell behavior. Two different cell lines cultured on the nanopillars show striking differences in cell coverage. These changes are topography‐ and cell‐dependent, and are not mediated by air bubbles trapped on the nanopattern. For the first time, a strong cell‐selective effect of the same nanotopography has been clearly demonstrated on a large area; while fibroblast proliferation is inhibited, endothelial cell spreading is visibly enhanced. The reduced fibroblast proliferation indicates that a reduction of available surface area induced by nanotopography might be the main factor affecting cell growth on nanopatterns. The results presented herein pave the way towards the development of permanent vascular replacements, where non‐adhesive, inert, surfaces will induce rapid in situ endothelialization to reduce thrombosis and occlusion.
Microfluidic Devices for Bioapplications
Authors: Yeo Leslie Y. , Chang Hsueh‐Chia , Chan Peggy P. Y. , Friend James R. 
Published: November 2010

AbstractHarnessing the ability to precisely and reproducibly actuate fluids and manipulate bioparticles such as DNA, cells, and molecules at the microscale, microfluidics is a powerful tool that is currently revolutionizing chemical and biological analysis by replicating laboratory bench‐top technology on a miniature chip‐scale device, thus allowing assays to be carried out at a fraction of the time and cost while affording portability and field‐use capability. Emerging from a decade of research and development in microfluidic technology are a wide range of promising laboratory and consumer biotechnological applications from microscale genetic and proteomic analysis kits, cell culture and manipulation platforms, biosensors, and pathogen detection systems to point‐of‐care diagnostic devices, high‐throughput combinatorial drug screening platforms, schemes for targeted drug delivery and advanced therapeutics, and novel biomaterials synthesis for tissue engineering. The developments associated with these technological advances along with their respective applications to date are reviewed from a broad perspective and possible future directions that could arise from the current state of the art are discussed.
Nanogeometry Matters: Unexpected Decrease of Capillary Adhesion Forces with Increasing Relative Humidity
Authors: Köber Mariana , Sahagún Enrique , García‐Mochales Pedro , Briones Fernando , Luna Monica , Sáenz Juan José 
Published: November 2010

AbstractThe sticking effect between hydrophilic surfaces occurring at increasing relative humidity (RH) is an everyday phenomenon with uncountable implications. Here experimental evidence is presented for a counterintuitive monotonous decrease of the capillary adhesion forces between hydrophilic surfaces with increasing RH for the whole humidity range. It is shown that this unexpected result is related to the actual shape of the asperity at the nanometer scale: a model based on macroscopic thermodynamics predicts this decrease in the adhesion force for a sharp object ending in an almost flat nanometer‐sized apex, in full agreement with experiments. This anomalous decrease is due to the fact that a significant growth of the liquid meniscus formed at the contact region with increasing humidity is hindered for this geometry. These results are relevant in the analysis of the dynamical behavior of nanomenisci. They could also have an outstanding value in technological applications, since the undesirable sticking effect between surfaces occurring at increasing RH could be avoided by controlling the shape of the surface asperities at the nanometric scale.