bluuebunny/crossref_metadata_2025_split huggingface dataset:
A giant planet in orbit around a magnetic-braking hibernating cataclysmic variable
Authors: Qian S.-B. , Liao W.-P. , Zhu L.-Y. , Dai Z.-B. , Liu L. , He J.-J. , Zhao E.-G. , Li L.-J. 
Published: November 2009

Abstract
Detections of Jupiter-like giant planets in orbit around short-period white dwarf binaries should provide insight into the formation and evolution of circumbinary planets (planets orbiting both components of short-period binaries), as well as into the ultimate fate of planets and the late evolutionary stage of binary stars (e.g. the evolution of the common envelope). However, to date no planets have been detected as companions to such close binaries. Here, we report the discovery of a giant planet orbiting the only known hibernating cataclysmic variable (CV), QS Vir, with a period of 7.86 yr. We analysed the variations of the orbital period of the eclipsing white dwarf–red dwarf binary, and a very small-amplitude cyclic change is found to be superimposed on a long-term period decrease. The period oscillation has the smallest amplitude among close binary stars and can be plausibly interpreted as the light-travel time effect via the presence of a third body. We found that the tertiary component is a giant planet with a mass of ∼6.4 MJupiter at a distance of ∼4.2 astronomical units (au) from the binary. The continuous decrease is explained as angular momentum loss via magnetic braking which is driving the evolution of the hibernating CV into a normal cataclysmic binary.
Escherichia coli: Dominance of Red Light over Other Visible Light Sources in Establishing Viable but Nonculturable State
Authors: İdil Önder , Özkanca Reşit , Darcan Cihan , Flint Ken P. 
Published: November 2009

AbstractIn this study, the effect of UV‐A and different wavelengths of visible light irradiations combined with or without a photosensitizer (methylene blue, MB) on the establishment of viable but nonculturable (VBNC) state in Escherichia coli was investigated. Survival of the E. coli was investigated by measuring plate counts, respiring cell count (RCC), direct viable count (DVC) and total counts over a period of up to 72 h. The inhibition rates of various light sources in the presence or absence of MB on E. coli in seawater were ranked in the order UV‐A>red light>white light>blue light>green light (from greatest to least activation). E. coli survived for 10.2, 19.0, 21.3 and 24.04 h under exposure to red, white, blue and green light and for 6.8 h under exposure to UV‐A in the presence of MB according to t99. Although the VC declined to undetectable levels in a relatively short time, the RCC showed that some cells were still capable of respiration and, therefore, are assumed to have entered the VBNC phase. This is the first time that red light has been shown to have a stronger effect on E. coli survival and VBNC than white, green and blue light in seawater environment.
New Discoveries From the Bilingual Brain and Mind Across the Life Span: Implications for Education
Authors: Petitto Laura‐Ann 
Published: November 2009

ABSTRACTWe discuss the fruits of educational neuroscience research from our laboratory and show how the typical maturational timing milestones in bilingual language acquisition provide educators with a tool for differentiating a bilingual child experiencing language and reading delay versus deviance. Further, early schooling in two languages simultaneously affords young bilingual children a reading advantage and may also ameliorate the negative effect of low socioeconomic status on literacy. Using powerful brain imaging technology, functional Near Infrared Spectroscopy, we provide a first‐time look into the developing brains of bilingual as comapred to monolingual children. We show unequivocally that the age of first bilingual exposure is a vital predictor of bilingual language and reading mastery. Accounts that promote later dual language and reading instruction, or those that assert human brain development is unrelated to bilingual language mastery, are not supported by the present findings. We discuss the implications for education, teachers, and developmental brain sciences.
A 30‐Minute Physical Education Program Improves Students' Executive Attention
Authors: Kubesch Sabine , Walk Laura , Spitzer Manfred , Kammer Thomas , Lainburg Alyona , Heim Rüdiger , Hille Katrin 
Published: November 2009

ABSTRACTPhysical activity is not only beneficial to physical health but also to cognitive functions. In particular, executive functions that are closely related to learning achievement can be improved by acute and recurring physical activity. We examined the effects of a single 30‐min physical education program in contrast to a 5‐min movement break on working memory, cognitive flexibility, and inhibition of attention and behavioral tendencies of eighty‐one 13‐ to 14‐year‐old students in grade 7 in Germany. Results indicate that the maintenance of on‐task attention in the face of distraction was improved by an aerobic endurance exercise‐based physical education program but not by a short aerobic movement break. This suggests that the duration of a school sports program is decisive for improving students' executive attention.
Perineurioma of the mandibular dental nerve: a case report and review of the literature
Authors: Vencio E.F. , Cheim Jr A.P. , Alencar R.C. , Alencar W.M. , Dias Filho A.A. 
Published: November 2009

AbstractPerineurioma is an uncommon benign tumour of the nerve sheath composed exclusively of well‐differentiated perineurial cells. It usually affects the limbs or trunk; intraoral lesions are uncommon. A rare case of perineurioma is described involving the mandibular dental nerve in a 59‐year‐old female, microscopically characterised by a combined pattern of intraneural and soft tissue perineurioma. The patient was referred for treatment of a lesion in the mandible. Panoramic radiography revealed a well‐delimited osteolytic lesion with radiopaque halo in an edentulous region of the left mandible. Occlusal view showed no expansion of cortical bone. A surgical procedure for incisional biopsy disclosed a connection between the tumour and the alveolar inferior nerve. Grossly, an enlargement of the affected nerve was described. Microscopic examination showed tumour cells arranged in pseudo‐onion bulb structures, with scattered collagen bundles, and areas with a whorled (storiform) arrangement. Many concentric whorls of spindle cells exhibiting thin, elongated eosinophic processes were seen. Immunohistochemical study exhibited strong positivity for epithelial membrane antigen. We report a rare case of perineurioma affecting the mandibular dental nerve showing both intraneural and soft tissue microscopic patterns and discuss morphological aspects for differential diagnosis.
Peripheral keratocystic odontogenic tumour: a case report
Authors: Jinbu Y. , Ueno Y. , Obi Y. , Ikeda K. , Kusama M. , Tsukinoki K. 
Published: November 2009

AbstractPeripheral keratocystic odontogenic tumour is a rare disease occurring in the gingiva and displaying histopathological characteristics similar to keratocystic odontogenic tumour. The patient was a 63‐year‐old man complaining of gingival swelling in the mandibular premolar region. A raised mass lesion 15 mm in diameter covered with normal epithelium was observed on the buccal gingiva of the left mandibular premolar region. Panoramic radiography and computed tomography excluded intraosseous cyst or tumour. Needle aspiration examination revealed paste‐like, pale yellow contents. The raised lesion was surgically resected. Histopathologically, the cyst wall comprised squamous epithelium with parakeratosis. Peripheral keratocystic odontogenic tumour was diagnosed.
p53 mutations in keratocystic odontogenic tumour
Authors: Kuroyanagi N. , Machida J. , Sakuma H. , Miyabe S. , Hashimoto O. , Yokoi M. , Warnakulasuriya S. , Nagao T. , Shimozato K. 
Published: November 2009

AbstractAim:  The abrogation of the function for the tumour suppressor gene p53 has been suggested as one of the most common genetic alterations in neoplasia. We evaluated p53 mutations, clinico‐pathological and immunohistochemical findings in patients with sporadic keratocystic odontogenic tumours (KCOTs).Material and methods:  A total of 32 patients with KCOT treated surgically were enrolled in this study. We performed mutation analysis of p53 using archival formalin‐fixed paraffin‐embedded tissue. The immunohistological technique was employed to identify TP53 expression. P53 mutations in exons 5–8 were evaluated by direct sequencing. These results were compared with the characteristics of each tumour.Results:  Forty‐four percent of the cysts showed overexpression of TP53. P53 mutations were detected in 10 cases (31.3%) of 32 KCOTs; two cases within exon 5, two cases within exon 8, five cases within exon 7 and one case in both exons 5 and 7. Five missense mutations (R248G, D247K, Q136R, V272M, V143A) and four silence mutations (138A, 158R, 247D, 279G,) were detected. P53 mutation was detected in two (50%) out of four recurrence cases and eight (28.5%) out of 28 non‐recurrence cases (P = 0.572). R248G mutation was the most common in KCOTs (15.6%). We did not detect any remarkable associations of these cases carrying p53 mutations to clinico‐pathological and immunohistochemical characteristics.Conclusion:  The results implied that the p53 gene alterations are involved in the development of KCOT.
Valuing and Caring
Authors: SEIDMAN JEFFREY 
Published: November 2009

AbstractWhat is it to “value” something, in the semi‐technical sense of the term that Gary Watson establishes? I argue that valuing something consists incaringabout it. Caring involves not only emotional dispositions of the sort that Agnieszka Jaworska has elaborated, but also a distinctivecognitivedisposition – namely, a (defeasible) disposition to believe the object cared about to be a source of agent‐relative reasons for action and for emotion. Understood in this way, an agent's carings have a stronger claim to “speak for” her as her values than do other attitudes that have been proposed for this role. In particular, an agent's carings establish more robust psychological continuities and cross‐temporal connections than do self‐governing policies of the sort that Michael Bratman has described; and they forge diachronic coherence not just in her deliberation and action, as self‐governing policies do, but also in her cognitive and emotional life. An agent's carings thus help to constitute her identity as a temporally persistingsubject. Self‐governing policies are at bestersatz‐values, which an agent may choose to adopt when she finds that her proper values – her cares – leave her course underdetermined.
Pelvic Autonomic Dysfunction without Tetraparesis: A Sequel of Rubella‐related Acute Longitudinal Myelitis
Authors: TAKAHASHI Osamu , SAKAKIBARA Ryuji , KISHI Masahiko , OGAWA Emina , TSUNOYAMA Kuniko , SUGIYAMA Megumi , TOMARU Takanobu , UCHIYAMA Tomoyuki , YAMANISHI Tomonori 
Published: November 2009

Objectives: Pelvic autonomic dysfunction without tetraparesis can be a sequel of acute longitudinal myelitis.Methods: A 25‐year‐old woman had suffered from pelvic dysfunction (urinary retention and severe constipation) without tetraparesis for 5 months as a sequel of rubella‐related acute longitudinal myelitis at the spinal segments of C2 to T9. She still had brisk deep tendon reflexes and extensor plantar responses. We performed autonomic function tests in the patient.Results: Pelvic autonomic function tests revealed acontractile detrusor and unrelaxing sphincter, suggesting the shock phase of a suprasacral spinal cord lesion; as well as loss of voluntary sphincter contraction, prolonged colonic transit time, decreased spontaneous phasic contractions, and paradoxical sphincter contraction on defecation, suggesting a suprasacral cord lesion.Conclusion: These findings indicated that the lateral medullary reticulospinal tracts just inside the pyramidal tract, for example, the descending pathways to the bladder and bowel, and the adjacent corticospinal tract to the sphincter, were selectively affected in our case.
Molecular Detection of Leishmania infantum in Wild Rodents (Rattus norvegicus) in Greece
Authors: Papadogiannakis E. , Spanakos G. , Kontos V. , Menounos P. G. , Tegos N. , Vakalis N. 
Published: November 2009

Summary Leishmania infantum and Leishmania tropica are the species responsible for visceral leishmaniasis and cutaneous leishmaniasis respectively. In Greece, both diseases are endemic. The dog is considered the main reservoir of L. infantum, whereas the role of other animals for both L. infantun and L. tropica is unknown. Spleens from wild Rattus norvegicus, live trapped in Greece, were examined for the presence of Leishmania parasites by PCR. Out of 16 samples examined, only one was found positive for L. infantum with scant amount of parasitic DNA present. This is the first documented case of detection of L. infantum in R. norvegicus in Greece. The results of this preliminary study indicate that R. norvegicus is unlikely to be a reservoir for Leishmania parasites in Greece.
Comparison of Three Antigenic Extracts of
Eurotium amstelodami
in Serological Diagnosis of Farmer's Lung Disease
Authors: Roussel Sandrine , Reboux Gabriel , Rognon Bénédicte , Monod Michel , Grenouillet Frédéric , Quadroni Manfredo , Fellrath Jean-Marc , Aubert John-David , Dalphin Jean-Charles , Millon Laurence 
Published: November 2009

ABSTRACT

In France and Finland, farmer's lung disease (FLD), a hypersensitivity pneumonitis common in agricultural areas, is mainly caused by
Eurotium
species. The presence of antibodies in patients' serum is an important criterion for diagnosis. Our study aimed to improve the serological diagnosis of FLD by using common fungal particles that pollute the farm environment as antigens. Fungal particles of the
Eurotium
species were observed in handled hay. A strain of
Eurotium amstelodami
was grown
in vitro
using selected culture media; and antigen extracts from sexual (ascospores), asexual (conidia), and vegetative (hyphae) forms were made. Antigens were tested by enzyme-linked immunosorbent assay (ELISA), which was used to test for immunoglobulin G antibodies from the sera of 17 FLD patients, 40 healthy exposed farmers, and 20 nonexposed controls. The antigens were compared by receiver operating characteristic analysis, and a threshold was then established. The ascospores contained in asci enclosed within cleistothecia were present in 38% of the hay blades observed; conidial heads of aspergillus were less prevalent. The same protocol was followed to make the three antigen extracts. A comparison of the results for FLD patients and exposed controls showed the area under the curve to be 0.850 for the ascospore antigen, 0.731 for the conidia, and 0.690 for the hyphae. The cutoffs that we determined, with the standard deviation for measures being taken into account, showed 67% for sensitivity and 92% for specificity with the ascospore antigen. In conclusion, the serological diagnosis of FLD by ELISA was improved by the adjunction of ascospore antigen.
Highly Penicillin-Resistant Multidrug-Resistant Pneumococcus-Like Strains Colonizing Children in Oeiras, Portugal: Genomic Characteristics and Implications for Surveillance
Authors: Simões Alexandra S. , Sá-Leão Raquel , Eleveld Marc J. , Tavares Débora A. , Carriço João A. , Bootsma Hester J. , Hermans Peter W. M. 
Published: November 2009

ABSTRACT

While performing surveillance studies in Oeiras, Portugal, designed to describe the impact of pneumococcal conjugate vaccine on colonization, we observed an increase from 0.7% in 2003 to 5% in 2006 in the prevalence of penicillin resistance (MIC of 2 to 6 mg/liter) among presumptively identified pneumococcal isolates. Although 15 of the 22 penicillin-resistant isolates obtained in 2006 were optochin resistant, they were bile soluble and thus considered to be bona fide pneumococci. This study aimed to clarify the nature of these isolates by using a combination of phenotypic and genotypic approaches that included routine strategies for pneumococcal identification, multilocus sequence analysis (MLSA), and comparative genomic hybridization (CGH). By MLSA, all isolates were classified as “streptococci of the mitis group” that, however, were distinct from typical
Streptococcus pneumoniae
or
Streptococcus mitis
. A single isolate was identified as
Streptococcus pseudopneumoniae
. CGH confirmed these findings and further indicated that a considerable part of the proposed pneumococcal core genome is conserved in these isolates, including several pneumococcal virulence genes (e.g.,
pavA
,
spxB
,
cbpE
, and
cbpD
). These results suggest that among pneumococci and closely related streptococci, universal unique phenotypic and genetic properties that could aid species identification are virtually impossible to define. In pneumococcal colonization studies, when atypical strains are found, MLSA and CGH are informative tools that can be used to complement routine tests. In our study, after correct identification of the penicillin-resistant true pneumococci, we found that penicillin resistance levels among pneumococci remained stable from 2003 to 2006.
Determining Cystic Fibrosis-Affected Lung Microbiology: Comparison of Spontaneous and Serially Induced Sputum Samples by Use of Terminal Restriction Fragment Length Polymorphism Profiling
Authors: Rogers Geraint B. , Skelton Stuart , Serisier David J. , van der Gast Christopher J. , Bruce Kenneth D. 
Published: November 2009

ABSTRACT
Sampling of the lower airways of the adult cystic fibrosis (CF) lung has received insufficient detailed consideration, with the importance of sampling strategies for bacteriological outcome not known. Although spontaneously expectorated sputum (SES) samples are often used for diagnostic bacteriological analysis, induced sputum (IS) methods have advantages. This study examined whether significant differences in bacterial content were detected when using a culture-independent, molecular profiling technique to analyze SES or IS samples. Moreover, this work examined what trends relating to bacterial species distributions and reproducibility were found in sequentially induced sputum samples and what implications this has for pathogen detection. Terminal restriction fragment length polymorphism (T-RFLP) analysis was performed on a SES sample and 4 subsequent IS samples taken at 5-min intervals from 10 clinically stable, adult CF patients. This was repeated over 3 sampling days, with variability between samples, induction periods, and sampling days determined. A diverse range of bacterial species, including potentially novel pathogens, was found. No significant difference in bacterial content was observed for either SES or serial IS samples. On average, the analysis of a single sample from any time point resolved ∼58% of total bacterial diversity achieved by analysis of an SES sample and 4 subsequent IS samples. The reliance on analysis of a single respiratory sample was not sufficient for the detection of recognized CF pathogens in all instances. Close correlation between T-RFLP and microbiological data in the detection of key species indicates the importance of these findings in routine diagnostics for the detection of recognized and novel CF pathogens.
Detection of Occult
Scedosporium
Species in Respiratory Tract Specimens from Patients with Cystic Fibrosis by Use of Selective Media
Authors: Blyth C. C. , Harun A. , Middleton P. G. , Sleiman S. , Lee O. , Sorrell T. C. , Meyer W. , Chen S. C. A. 
Published: November 2009

ABSTRACT

Respiratory samples from cystic fibrosis outpatients were cultured on Sabouraud's dextrose agar (SABD) containing antibiotics, Mycosel, and
Scedosporium
-selective medium (SceSel+). Thirty-two (14.7%) of 218 specimens from 11/69 (15.9%) patients yielded a
Scedosporium
sp., most frequently
Scedosporium aurantiacum
(17/218).
Scedosporium
was recovered on SceSel+, Mycosel, and SABD from 90.6%, 50.0%, and 46.9% of the specimens tested, respectively.
Commercial DNA Probes for Mycobacteria Incorrectly Identify a Number of Less Frequently Encountered Species
Authors: Tortoli Enrico , Pecorari Monica , Fabio Giuliana , Messinò Massimino , Fabio Anna 
Published: November 2009

ABSTRACTAlthough commercially available DNA probes for identification of mycobacteria have been investigated with large numbers of strains, nothing is known about the ability of these probes to identify less frequently encountered species. We analyzed, with INNO LiPA MYCOBACTERIA (Innogenetics) and with GenoType Mycobacterium (Hein), 317 strains, belonging to 136 species, 61 of which had never been assayed before. INNO LiPA misidentified 20 taxa, the majority of which cross-reacted with the probes specific forMycobacterium fortuitumand theMycobacterium avium-Mycobacterium intracellulare-Mycobacterium scrofulaceumgroup. GenoType misidentified 28 taxa, most of which cross-reacted withM. intracellulareandM. fortuitumprobes; furthermore, eight species were not recognized as members of the genusMycobacterium. Among 54 strains investigated with AccuProbe (Gen-Probe), cross-reactions were detected for nine species, with the probes aiming at theM. aviumcomplex being most involved in cross-reactions.
Internal Transcribed Spacer Region Sequence Heterogeneity in
Rhizopus microsporus
: Implications for Molecular Diagnosis in Clinical Microbiology Laboratories
Authors: Woo Patrick C. Y. , Leung Shui-Yee , To Kelvin K. W. , Chan Jasper F. W. , Ngan Antonio H. Y. , Cheng Vincent C. C. , Lau Susanna K. P. , Yuen Kwok-Yung 
Published: November 2009

ABSTRACT

Although internal transcribed spacer region (ITS) sequence heterogeneity has been reported in a few fungal species, it has very rarely been reported in pathogenic fungi and has never been described in
Mucorales
, causes of the highly fatal mucormycosis. In a recent outbreak investigation of intestinal mucormycosis due to
Rhizopus microsporus
infection in patients with hematological malignancies, PCR of the ITS of four of the 28
R. microsporus
strains, P11, P12, D3-1, and D4-1, showed thick bands at about 700 bp. Direct sequencing of the purified bands showed frequent double peaks along all of the sequence traces and occasional triple peaks for P12, D3-1, and D4-1. The thick bands of the four
R. microsporus
strains were purified and cloned. Sequencing of 10 clones for each strain revealed two different ITS sequences for P11 and three different ITS sequences for P12, D3-1, and D4-1. Variations in ITS sequence among the different ribosomal DNA (rDNA) operons in the same strain were observed in only ITS1 and ITS2 and not the 5.8S rDNA region. One copy of P11, P12, and D4-1, respectively, and one copy of P11, P12, D3-1, and D4-1, respectively, showed identical sequences. This represents the first evidence of ITS sequence heterogeneity in
Mucorales
. ITS sequence heterogeneity is an obstacle to molecular identification and genotyping of fungi in clinical microbiology laboratories. When thick bands and double peaks are observed during PCR sequencing of a gene target, such a strain should be sent to reference laboratories proficient in molecular technologies for further identification and/or genotyping.