bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ingrid Arnet Connidis. Family Ties and Aging. Toronto: Butterworths, 1989, pp. 130.
Authors: Kivett Vira R. 
Published: December 2010

RÉSUMÉFamily Ties and Aging incorpore et résume quelques unes des plus récentes études de recherche portant sur les liens de famille verticaux et horizontaux à partir d'une perspective occidentale. Le texte est soigneusement rédigé, succinct et offre une bonne perspective démographique des adultes canadiens âgés. L'auteur se reporte et incorpore dans son texte plusieurs enquêtes canadiennes d'envergure portant sur des personnes âgées dans des contextes familiaux. Le livre de Connidis ressemble quelque peu à certains textes américains moins récents sur la famille d'âge mûr. Cependant, il se distingue car l'auteur se concentre sur la personne plutôt que sur la famille et s'organise autour de liens de famille verticaux et horizontaux plutôt qu'autour de la situation de famille. Ce livre s'adresse à une grande variété de personnes, à partir du simple lecteur jusqu'au praticien, à l'enseignant et au chercheur. Chacun pourra y trouver une part de valeur plus ou moins utile. Les sujets sont variés, sinon approfondis et touchent la plupart des questions importantes concernant les relations familiales chez les personnes âgées. Une comparaison entre les données canadiennes et américaines met en relief certaines ressemblances. L'auteur cependant ne s'étend pas sur la variabilité et la théorie ethnique et raciale. Ce petit livre intéressera sûrement les étudiants inscrits à des cours de brève durée ou à des séminaires sur les relations familiales d'âge mûr.
Brachial plexus injury as an unusual complication after aortic stent implantation
Authors: Sabiniewicz Robert , Ereciński Jan , Zipser Mariusz 
Published: December 2010

AbstractAorta catheterisation can serve both diagnostic and therapeutic purposes in patients with aortic coarctation, especially when non-invasive evaluation cannot define severity of the abnormality. We report a case of bilateral brachial plexus injury due to standard positioning of the patient’s arms during non-complicated aorta catheterisation and aortic stent implantation under general anaesthesia. In discussion, we consider our patient’s predisposing factors, the understanding of which may be helpful in avoiding this complication in the future.
Mucolipidosis type IV and the mucolipins
Authors: Bach Gideon , Zeevi David A. , Frumkin Ayala , Kogot-Levin Aviram 
Published: December 2010

MLIV (mucolipidosis type IV) is a neurodegenerative lysosomal storage disorder caused by mutations in MCOLN1, a gene that encodes TRPML1 (mucolipin-1), a member of the TRPML (transient receptor potential mucolipin) cation channels. Two additional homologues are TRPML2 and TRPML3 comprising the TRPML subgroup in the TRP superfamily. The three proteins play apparently key roles along the endocytosis process, and thus their cellular localization varies among the different group members. Thus TRPML1 is localized exclusively to late endosomes and lysosomes, TRPML2 is primarily located in the recycling clathrin-independent GPI (glycosylphosphatidylinositol)-anchored proteins and early endosomes, and TRPML3 is primarily located in early endosomes. Apparently, all three proteins' main physiological function underlies Ca2+ channelling, regulating the endocytosis process. Recent findings also indicate that the three TRPML proteins form heteromeric complexes at least in some of their cellular content. The physiological role of these complexes in lysosomal function remains to be elucidated, as well as their effect on the pathophysiology of MLIV. Another open question is whether any one of the TRPMLs bears additional function in channel activity
Induction of chromosome malsegregation by nanomaterials
Authors: Gonzalez Laetitia , Decordier Ilse , Kirsch-Volders Micheline 
Published: December 2010

NMs (nanomaterials), defined as materials with at least one dimension smaller than 100 nm, are able to induce genotoxic effects. One of the hypotheses of the mode-of-action in which they exert their genotoxic potential is to mechanically interfere with subcellular structures, in particular the microtubules. In the present paper, we review studies exploring interactions between NMs and tubulin; therefore a PubMed literature search was performed. From this search 12 studies, applying both acellular and cellular assays, were retrieved and are summarized according to endpoint and particle type. These studies show that there are interactions between different types of NMs and tubulins in both acellular and cellular systems. For several types of NMs, the multi-walled carbon nanotubes, amorphous SiO2, TiO2 and CoCr, an induction of aneuploidy was observed in vitro. There is, therefore, a critical need to assess the capacity of NMs to interfere with the cytoskeleton, and in particular the tubulins. This might require definition of relevant dosimetry, adaptations of some testing protocols, possibly development of new methodologies and studies on a larger size-range of NMs.
Group Intervention for Adolescents With Chronic Acquired Brain Injury: The Future Zone
Authors: Hickey Ellen M. , Saunders Jennifer N. 
Published: December 2010

Introduction: Acquired brain injury (ABI) is a common cause of social cognition deficits and psychosocial challenges in adolescents. A group intervention program was designed to support teens with chronic ABI.
Method: Quantitative and qualitative analyses were conducted to examine participants' self-knowledge and satisfaction with the program.
Results: Results revealed that participants and parents valued increased self-knowledge and an understanding of brain injuries, the focus on friendships and individual strengths, and feelings of togetherness and not being judged.
Discussion: Support for strength-based, project-oriented, group intervention programs for adolescents with ABI and directions for future research are discussed.
Europe 1992
Authors: Leitner Gerhard 
Published: November 2010

ZUSAMMENFASSUNG
Europa 1992: eine sprachliche Perspektive
Die Europaische Gemeinschaft praktiziert seit ihrer Griindung im Jahre 1952 eine föderalistische Sprachformel. Der Druck hin zu einer (mehr) zentralistischen, hegemonialen Praxis ist allerdings sehr stark. Abgesehen von den enormen Kosten, die die fôderalistische Formel nach sich zieht, ist sie nicht voll im Einklang mit den Erziehungssystemen, d.h. Mitgliedstaaten, den Einstellungen der öffentlichkeit und den großeren internationalen Zusammenhängen. Und dennoch scheint es keine Alternative innerhalb einer föderalistischen europ äischen Union zu geben, die im Jahre 1992 verwirklicht werden soil.
Eine föderalistische Sprachformel hat daher eine grofie Uberlebenschance, wenn sie modifiziert, vielleicht eingeschrankt wird, so daß sie funktioneller und fur die Erfordernisse der weltweiten Kommunikation offener ist. Trotz ihrer Schwâchen spiegelt sie das mosaikhafte kulturelle Erbe Europas in adäquaterer Weise wider. Sie ist auch für andere Regionen der Welt und für internationale Institutionen, die eine zentralistische Politik verfolgen, von Interesse, vor allem dann, wenn die Kosten ihrer Umsetzung in die Praxis vermindert werden können.
RESUMO
Europo 1992: lingva perspektivo
De kiam gi fondigis en 1952 la Eûropa Komunumo praktikas federacian lingvoaplikon. Tamen ekzistas tre forta premo al (pli) centrisma, hegemonia praktiko. Ec senkonsidere de giaj altaj kostoj la federacia lingvoapliko malkongruas kun la eduksistemoj en la statoj-membroj, la sinteno en la civitanaro, kaj la pli vasta internacia konteksto. Malgraû ci cio sajnas ne ekzisti alia solvo en la kadro de federacia eûropa unio, kiun oni planas realigi en 1992.
Pro tio federacia lingvoapliko havas bonan sancon pluvivi, se oni modifas au eble limigas gin tiel ke |i farigu pli funkcüva kaj pli taûga por tutmonda komunikado. Malgraû siaj malavantagoj tia solvo adekvate spegulas la mozaikecan kulturheredajon de Eûropo. Gi estas interesa ankaû por aliaj partoj de la mondo kaj por intemaciaj organizajoj kiuj aplikas centrisman politikon, precipe se oni sukcesos redukti la kostojn de gia realigo.
One Thousand Endoscopic Skull Base Surgical Procedures Demystifying the Infection Potential: Incidence and Description of Postoperative Meningitis and Brain Abscesses
Authors: Kono Yuriko , Prevedello Daniel M. , Snyderman Carl H. , Gardner Paul A. , Kassam Amin B. , Carrau Ricardo L. , Byers Karin E. 
Published: December 2010

Background.Endonasal endoscopic skull base surgery (ESBS) is perceived as having a high risk of infection because it is performed through the sinuses, which are not sterile.Objective.To identify the bacteriological characteristics, incidence, mortality, and risk factors for intracranial infection after ESBS.Methods.A retrospective analysis of the first 1,000 ESBS procedures performed at the University of Pittsburgh Medical Center from 1998 to 2008.Results.In 18 cases (1.8%), the patient developed meningitis. In 2 cases, the patient died within 2 months after surgery, of noninfectious causes. In 11 cases, cerebrospinal fluid (CSF) cultures had positive results. There were no predominant pathogens. Male sex (odds ratio [OR], 3.97 [95% confidence interval {CI}, 1.21-13.03]; P = .02), history of a craniotomy or endonasal surgery (OR, 4.77 [95% CI, 1.68-13.56];P = .003), surgerywith higher levels of complexity (OR, 6.60 [95% CI, 1.77-24.70];P = .005), the presence of an external ventricular drain or ventriculoperitoneal shunt at the time of surgery (OR, 6.38 [95% CI, 1.07-38.09]; P = .04), and postoperative CSF leak (OR, 12.99 [95% CI, 4.24-39.82]; P<.001) were risk factors for infection.Conclusion.The incidence of infection of 1.8% in ESBS is comparable to that in open craniotomy. The most important risk factor was a postoperative CSF leak. All patients recovered from their infection.
High-quality annotation of promoter regions for 913 bacterial genomes
Authors: Rangannan Vetriselvi , Bansal Manju 
Published: December 2010

Abstract
Motivation: The number of bacterial genomes being sequenced is increasing very rapidly and hence, it is crucial to have procedures for rapid and reliable annotation of their functional elements such as promoter regions, which control the expression of each gene or each transcription unit of the genome. The present work addresses this requirement and presents a generic method applicable across organisms.
Results: Relative stability of the DNA double helical sequences has been used to discriminate promoter regions from non-promoter regions. Based on the difference in stability between neighboring regions, an algorithm has been implemented to predict promoter regions on a large scale over 913 microbial genome sequences. The average free energy values for the promoter regions as well as their downstream regions are found to differ, depending on their GC content. Threshold values to identify promoter regions have been derived using sequences flanking a subset of translation start sites from all microbial genomes and then used to predict promoters over the complete genome sequences. An average recall value of 72% (which indicates the percentage of protein and RNA coding genes with predicted promoter regions assigned to them) and precision of 56% is achieved over the 913 microbial genome dataset.
Availability: The binary executable for ‘PromPredict’ algorithm (implemented in PERL and supported on Linux and MS Windows) and the predicted promoter data for all 913 microbial genomes are available at http://nucleix.mbu.iisc.ernet.in/prombase/.
Contact: mb@mbu.iisc.ernet.in
Supplementary information: Supplementary data are available at Bioinformatics online.
Flapjack—graphical genotype visualization
Authors: Milne Iain , Shaw Paul , Stephen Gordon , Bayer Micha , Cardle Linda , Thomas William T. B. , Flavell Andrew J. , Marshall David 
Published: December 2010

Abstract
Summary: New software tools for graphical genotyping are required that can routinely handle the large data volumes generated by the high-throughput single-nucleotide polymorphism (SNP) platforms, genotyping-by-sequencing and other comparable genotyping technologies. Flapjack has been developed to facilitate analysis of these data, providing real time rendering with rapid navigation and comparisons between lines, markers and chromosomes, with visualization, sorting and querying based on associated data, such as phenotypes, quantitative trait loci or other mappable features.
Availability: Flapjack is freely available for Microsoft Windows, Mac OS X, Linux and Solaris, and can be downloaded from http://bioinf.scri.ac.uk/flapjack
Contact: flapjack@scri.ac.uk
HiTEC: accurate error correction in high-throughput sequencing data
Authors: Ilie Lucian , Fazayeli Farideh , Ilie Silvana 
Published: November 2010

Abstract
Motivation: High-throughput sequencing technologies produce very large amounts of data and sequencing errors constitute one of the major problems in analyzing such data. Current algorithms for correcting these errors are not very accurate and do not automatically adapt to the given data.
Results: We present HiTEC, an algorithm that provides a highly accurate, robust and fully automated method to correct reads produced by high-throughput sequencing methods. Our approach provides significantly higher accuracy than previous methods. It is time and space efficient and works very well for all read lengths, genome sizes and coverage levels.
Availability: The source code of HiTEC is freely available at www.csd.uwo.ca/~ilie/HiTEC/.
Contact: ilie@csd.uwo.ca
Defolliculation of Xenopus Oocytes
Authors: Sive Hazel L. , Grainger Robert M. , Harland Richard M. 
Published: December 2010

INTRODUCTIONIt is possible to microinject Xenopus oocytes that are still contained within their ovarian follicles, but most researchers find it more convenient to work with defolliculated oocytes. Defolliculation can be carried out enzymatically by treatment with collagenase, or it can be performed manually. Enzymatic treatment is recommended for preparation of large numbers of oocytes (more than 1000); however, this treatment causes complications because it often damages the quality of the oocytes. The manual procedure, which requires some practice, is recommended for experiments requiring only a few hundred oocytes. This protocol details both the enzymatic and manual procedures for defolliculation of Xenopus oocytes.
An Agar Mount for Observation of Caenorhabditis elegans Embryos
Authors: Walston Timothy , Hardin Jeff 
Published: December 2010

INTRODUCTIONThe Caenorhabditis elegans embryo is particularly amenable to microscopy and embryological studies because of its short developmental time, transparent shell, and nonpigmented cells. The agar mount described in this protocol is an easy way to prepare live C. elegans embryos for microscopic visualization. The mount slightly embeds the embryo in agar to hold it in place. The mount also slightly compresses the embryo to provide consistent orientation such that every embryo will be positioned with either its right side or its left side facing the objective. Other techniques can result in random orientations that complicate analysis and make identification of individual blastomeres more challenging.
Laser Killing of Blastomeres in Caenorhabditis elegans
Authors: Walston Timothy , Hardin Jeff 
Published: December 2010

INTRODUCTIONThe Caenorhabditis elegans embryo is particularly amenable to microscopy and embryological studies because of its short developmental time, transparent shell, and nonpigmented cells. Within the embryo, contacts between cells often establish the polarization of neighboring cells. Blastomere isolation and recombination experiments have led to a wealth of understanding of the events in the four-cell C. elegans embryo. However, identifying individual blastomeres after isolation at stages past the four-cell stage is limited. In addition, removal of blastomeres from their native surroundings can interfere with many cell contacts besides the contacts of interest. An alternative approach for studying cell interactions within the C. elegans embryo is to use laser ablation of individual cells. Laser ablation can be used to kill one of two cells in contact with each other to understand what happens when a cell no longer signals to its neighbor. Additionally, killing a cell that is between two cells that will eventually contact each other can result in the corpse of the cell forming a steric barrier between the cells, preventing the contact. This protocol describes laser ablation of embryos mounted on an agar mount.
Molecular Evolution and Selection Patterns of Plant F-Box Proteins with C-Terminal Kelch Repeats
Authors: Schumann Nadine , Navarro-Quezada Aura , Ullrich Kristian , Kuhl Carsten , Quint Marcel 
Published: December 2010

Abstract
The F-box protein superfamily represents one of the largest families in the plant kingdom. F-box proteins phylogenetically organize into numerous subfamilies characterized by their carboxyl (C)-terminal protein-protein interaction domain. Among the largest F-box protein subfamilies in plant genomes are those with C-terminal kelch repeats. In this study, we analyzed the phylogeny and evolution of F-box kelch proteins/genes (FBKs) in seven completely sequenced land plant genomes including a bryophyte, a lycophyte, monocots, and eudicots. While absent in prokaryotes, F-box kelch proteins are widespread in eukaryotes. Nonplant eukaryotes usually contain only a single FBK gene. In land plant genomes, however, FBKs expanded dramatically. Arabidopsis thaliana, for example, contains at least 103 F-box genes with well-conserved C-terminal kelch repeats. The construction of a phylogenetic tree based on the full-length amino acid sequences of the FBKs that we identified in the seven species enabled us to classify FBK genes into unstable/stable/superstable categories. In contrast to superstable genes, which are conserved across all seven species, kelch domains of unstable genes, which are defined as lineage specific, showed strong signatures of positive selection, indicating adaptational potential. We found evidence for conserved protein features such as binding affinities toward A. thaliana SKP1-like adaptor proteins and subcellular localization among closely related FBKs. Pseudogenization seems to occur only rarely, but differential transcriptional regulation of close relatives may result in subfunctionalization.
Physiological Effects of the Synthetic Strigolactone Analog GR24 on Root System Architecture in Arabidopsis: Another Belowground Role for Strigolactones?
Authors: Ruyter-Spira Carolien , Kohlen Wouter , Charnikhova Tatsiana , van Zeijl Arjan , van Bezouwen Laura , de Ruijter Norbert , Cardoso Catarina , Lopez-Raez Juan Antonio , Matusova Radoslava , Bours Ralph , Verstappen Francel , Bouwmeester Harro 
Published: December 2010

AbstractIn this study, the role of the recently identified class of phytohormones, strigolactones, in shaping root architecture was addressed. Primary root lengths of strigolactone-deficient and -insensitive Arabidopsis (Arabidopsis thaliana) plants were shorter than those of wild-type plants. This was accompanied by a reduction in meristem cell number, which could be rescued by application of the synthetic strigolactone analog GR24 in all genotypes except in the strigolactone-insensitive mutant. Upon GR24 treatment, cells in the transition zone showed a gradual increase in cell length, resulting in a vague transition point and an increase in transition zone size. PIN1/3/7-green fluorescent protein intensities in provascular tissue of the primary root tip were decreased, whereas PIN3-green fluorescent protein intensity in the columella was not affected. During phosphate-sufficient conditions, GR24 application to the roots suppressed lateral root primordial development and lateral root forming potential, leading to a reduction in lateral root density. Moreover, auxin levels in leaf tissue were reduced. When auxin levels were increased by exogenous application of naphthylacetic acid, GR24 application had a stimulatory effect on lateral root development instead. Similarly, under phosphate-limiting conditions, endogenous strigolactones present in wild-type plants stimulated a more rapid outgrowth of lateral root primordia when compared with strigolactone-deficient mutants. These results suggest that strigolactones are able to modulate local auxin levels and that the net result of strigolactone action is dependent on the auxin status of the plant. We postulate that the tightly balanced auxin-strigolactone interaction is the basis for the mechanism of the regulation of the plants’ root-to-shoot ratio.
A Domain-Centric Analysis of Oomycete Plant Pathogen Genomes Reveals Unique Protein Organization
Authors: Seidl Michael F. , Van den Ackerveken Guido , Govers Francine , Snel Berend 
Published: December 2010

Abstract
Oomycetes comprise a diverse group of organisms that morphologically resemble fungi but belong to the stramenopile lineage within the supergroup of chromalveolates. Recent studies have shown that plant pathogenic oomycetes have expanded gene families that are possibly linked to their pathogenic lifestyle. We analyzed the protein domain organization of 67 eukaryotic species including four oomycete and five fungal plant pathogens. We detected 246 expanded domains in fungal and oomycete plant pathogens. The analysis of genes differentially expressed during infection revealed a significant enrichment of genes encoding expanded domains as well as signal peptides linking a substantial part of these genes to pathogenicity. Overrepresentation and clustering of domain abundance profiles revealed domains that might have important roles in host-pathogen interactions but, as yet, have not been linked to pathogenicity. The number of distinct domain combinations (bigrams) in oomycetes was significantly higher than in fungi. We identified 773 oomycete-specific bigrams, with the majority composed of domains common to eukaryotes. The analyses enabled us to link domain content to biological processes such as host-pathogen interaction, nutrient uptake, or suppression and elicitation of plant immune responses. Taken together, this study represents a comprehensive overview of the domain repertoire of fungal and oomycete plant pathogens and points to novel features like domain expansion and species-specific bigram types that could, at least partially, explain why oomycetes are such remarkable plant pathogens.