bluuebunny/crossref_metadata_2025_split huggingface dataset:
Realising the future: forecasting with high‐frequency‐based volatility (HEAVY) models
Authors: Shephard Neil , Sheppard Kevin 
Published: January 2010

AbstractThis paper studies in some detail a class of high‐frequency‐based volatility (HEAVY) models. These models are direct models of daily asset return volatility based on realised measures constructed from high‐frequency data. Our analysis identifies that the models have momentum and mean reversion effects, and that they adjust quickly to structural breaks in the level of the volatility process. We study how to estimate the models and how they perform through the credit crunch, comparing their fit to more traditional GARCH models. We analyse a model‐based bootstrap which allows us to estimate the entire predictive distribution of returns. We also provide an analysis of missing data in the context of these models. Copyright © 2010 John Wiley & Sons, Ltd.
Interaction of Sedlin with PAM14
Authors: Liu Xiaoying , Wang Yunfei , Zhu Hengrui , Zhang Qinghui , Xing Xin , Wu Biao , Song Lihua , Fan Libin 
Published: January 2010

AbstractSedlin is an evolutionarily conserved and ubiquitously expressed protein that is encoded by the gene SEDL. Mutations in the latter are known to be causative for spondyloepiphyseal dysplasia tarda. However, the mechanism underlying this remains unclear. We have previously shown that Sedlin interacts with the intracellular chloride channel proteins CLIC1 and CLIC2 in the cytoplasm. In this report we show that Sedlin is also physically associated with protein associated with MRG 14 kDa (PAM14), a nuclear protein that interacts with the transcription factor MORF4‐related gene on chromosome 15 (MRG15). This was suggested by yeast two‐hybrid screening and was confirmed with GST pull‐down and immunoprecipitation assays. Moreover, we demonstrate that the C‐terminus of Sedlin and the N‐terminus of PAM14 are critical for their interaction. Together, these results suggest that nucleus‐localized Sedlin may play a role in regulation of transcriptional activities of the MRG family of transcription factors via binding to PAM14. J. Cell. Biochem. 109: 1129–1133, 2010. © 2010 Wiley‐Liss, Inc.
Apoptosis‐inducing effect of garcinol is mediated by NF‐κB signaling in breast cancer cells
Authors: Ahmad Aamir , Wang Zhiwei , Ali Raza , Maitah Ma'in Y. , Kong Dejuan , Banerjee Sanjeev , Padhye Subhash , Sarkar Fazlul H. 
Published: January 2010

AbstractGarcinol, obtained from Garcinia indica in tropical regions, is used for its numerous biological effects. Its anti‐cancer activity has been suggested but the mechanism of action has not been studied in‐detail, especially there is no report on its action against breast cancer cells. Here we tested our hypothesis that garcinol may act as an anti‐proliferative and apoptosis‐inducing agent against breast cancer cell lines. Using multiple techniques such as MTT, Histone‐DNA ELISA, Annexin V‐PI staining, Western blot for activated caspases and cleaved PARP, homogenous caspase‐3/7 fluorometric assay and EMSA, we investigated the mechanism of apoptosis‐inducing effect of garcinol in ER‐positive MCF‐7 and ER‐negative MDA‐MB‐231 cells. We found that garcinol exhibits dose‐dependent cancer cell‐specific growth inhibition in both the cell lines with a concomitant induction of apoptosis, and has no effect on non‐tumorigenic MCF‐10A cells. Our results suggested induction of caspase‐mediated apoptosis in highly metastatic MDA‐MB‐231 cells by garcinol. Down‐regulation of NF‐κB signaling pathway was observed to be the mechanism of apoptosis‐induction. Garcinol inhibited constitutive NF‐κB activity, which was consistent with down‐regulation of NF‐κB‐regulated genes. This is the first report on anti‐proliferative and apoptosis‐inducing action of garcinol against human breast cancer cells and the results suggest that this natural compound merits investigation as a potential chemo‐preventive/‐therapeutic agent, especially against breast cancer. J. Cell. Biochem. 109: 1134–1141, 2010. © 2010 Wiley‐Liss, Inc.
Distinct mechanosensitive Ca2+influx mechanisms in human primary synovial fibroblasts
Authors: Sakamoto Yuko , Ishijima Muneaki , Kaneko Haruka , Kurebayashi Nagomi , Ichikawa Naoki , Futami Ippei , Kurosawa Hisashi , Arikawa‐Hirasawa Eri 
Published: January 2010

AbstractSynovial cells are exposed to continually changing dynamic forces and are implicated in the maintenance of joint homeostasis. However, the mechanisms of synovial cell responses to mechanical stress are unclear. In this study, we investigated the difference between the mechanosensitive channels of human primary synovial fibroblasts (SFBs) and human primary dermal fibroblasts (DFBs) in response to mechanical stretch by uniaxial cyclic stretching and mechanical cell membrane deformation in vitro. Cyclic stretching induced orientation of SFBs and DFBs perpendicularly to the stretching direction. Furthermore, uniaxial stretching increased intracellular Ca2+levels in both cell types. The perpendicular orientation of DFBs was blocked by gadolinium (III) chloride (Gd3+, a mechanosensitive Ca2+channel blocker) or ruthenium red (RR, a nonselective Ca2+channel blocker). However, Gd3+did not block the stretch‐induced perpendicular orientation in SFBs, while RR inhibited this orientation. Similarly, Ca2+influx in DFBs induced by uniaxial stretching and membrane deformation was inhibited by Gd3+, RR, and GsMTx‐4 (another mechanosensitive Ca2+channel blocker), while only RR inhibited Ca2+influx in SFBs. Our results suggest that SFBs respond to mechanical stretch through mechanosensitive channels that are distinct from those of DFBs. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:859–864, 2010
Intraneural blood flow analysis during an intraoperative Phalen's test in carpal tunnel syndrome
Authors: Yayama Takafumi , Kobayashi Shigeru , Awara Kousuke , Takeno Kenichi , Miyazaki Tsuyoshi , Kubota Masafumi , Negoro Kohei , Baba Hisatoshi 
Published: January 2010

AbstractPhalen's test has been one of the most significant of clinical signs when making a clinical diagnosis of idiopathic carpal tunnel syndrome (CTS). However, it is unknown whether intraneural blood flow changes during Phalen's test in patients with CTS. In this study, an intraoperative Phalen's test was conducted in patients with CTS to observe the changes in intraneural blood flow using a laser Doppler flow meter. During Phalen's test, intraneural blood flow showed a sharp decrease, which lasted for 1 min. Intraneural blood flow decreased by 56.7%–100% (average, 78.0%) in the median nerve relative to the blood flow before the test. At 1 min after completing the test, intraneural blood flow returned to the baseline value. After carpal tunnel release, there was no marked decrease in intraneural blood flow. This study demonstrated that the blood flow in the median nerve is reduced when Phalen's test is performed in vivo. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1022–1025, 2010
Comparison of differential biomarkers of osteoarthritis with and without posttraumatic injury in the Hartley guinea pig model
Authors: Wei Lei , Fleming Braden C. , Sun Xiaojuan , Teeple Erin , Wu Wesley , Jay Gregory D. , Elsaid Khaled A. , Luo Junming , Machan Jason T. , Chen Qian 
Published: January 2010

AbstractThe objective was to compare biomarkers of articular cartilage metabolism in synovial fluid from Hartley guinea pig knees, with and without anterior cruciate ligament transection (ACLT), to establish whether detectable differences in biomarker levels exist between primary and secondary osteoarthritis (OA). Synovial fluid lavages and knees were obtained from 3‐month (control group) and 12‐month (primary OA group) animals. Another group of animals (posttraumatic OA group) underwent unilateral ACLT at 3 months, and samples were obtained 9 months postsurgery. Synovial fluid concentrations of stromal cell‐derived‐factor (SDF‐1), collagen fragments (C2C), proteoglycan (GAG), lubricin, matrix metalloproteinase‐13 (MMP‐13), and Interleukin‐1 (IL‐1β) were evaluated. Cartilage damage was assessed via histology. The highest concentrations of C2C and SDF‐1 in synovial fluid were found in the posttraumatic OA group, moderate concentrations were found in the primary OA group, and low concentrations in the control group. GAG release in synovial fluid was similar to C2C and SDF‐1. The lubricin concentrations were significantly lower in ACLT joints than either the control or 12‐month primary OA groups, but not between the control and primary OA groups. Higher levels of MMP‐13 and IL‐1β were detected in the joints of the posttraumatic OA group as compared to the control or primary OA groups. Histology revealed greatest OA damage in the posttraumatic OA group, followed by moderate and minimal damage in primary OA and control groups, respectively. This study indicates that the biomarkers and progression of OA may differ in the Hartley guinea pig models with and without posttraumatic OA. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:900–906, 2010
Biphasic effects of interleukin‐1β on osteoblast differentiation in vitro
Authors: Lin Fu‐Hsiumg , Chang Jessica B. , McGuire Michael H. , Yee John A. , Brigman Brian E. 
Published: January 2010

AbstractA rat calvarial cell model of osteoblast differentiation using the formation of bone nodules in vitro as an endpoint was used to assess the effects of IL‐1β on osteoblast differentiation. Short‐term treatment (2 days) with IL‐1β early in culture resulted in increased nodule number and size as well as calcium content in contrast to long‐term treatment (6 days) in cultures assessed at 10–12 days. This increase in bone formation was blocked by IL‐1 receptor antagonists. Short‐term treatment increased COX‐2, prostaglandin (PGE2), and iNOS production. Exogenous PGE2 with IL‐1β enhanced this effect. COX‐2 inhibitors, indomethacin and N‐39, blocked 50% of nodule formation. NO donor did not modify effects of IL‐1β, but iNOS inhibitor (1400W) partially blocked the effects. However, PGE2 and NO donors could not rescue the decreased nodule number resulting from long‐term IL‐1β treatment. The results of this study suggest a biphasic effect of IL‐1β on bone nodule formation activated by IL‐1β binding with IL‐1 receptors, and the anabolic effect of early short‐term treatment with IL‐1β is likely mediated by PGE without ruling out nitric oxide. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:958–964, 2010
Hydrogen bonding in the pyrimidine/ formamide system: a concentration‐dependent Raman and DFT study
Authors: Srivastava Sunil K. , Schlücker Sebastian , Alves Wagner A. 
Published: January 2010

AbstractHigh‐resolution Raman spectra of pyrimidine (PD) and formamide (FA) mixtures with different compositions recorded in the ring breathing region of PD (ν1 ∼ 991 cm−1) are presented. The dilution of PD with FA leads to the appearance of a new band at ν1′ ∼ 994 cm−1, which is assigned to hydrogen‐bonded PD:FA species. From a quantitative analysis of the concentration‐dependent Raman spectra, the average number of FA molecules in the first solvation sphere of PD is determined as being equal to 2. This value is supported by density functional theory (DFT) calculations: a symmetric 1:2 complex is the most stable species among various hydrogen‐bonded PD:FA clusters with stoichiometries ranging from 1:1 to 1:4. A qualitative explanation for the blue shift of the ν1 mode upon complexation is given. Additionally, we have observed not only similarities but also some differences with respect to the PD:water system. Copyright © 2010 John Wiley & Sons, Ltd.
Surface‐sensitive Raman microscopy with total internal reflection illumination
Authors: Michaels Chris A. 
Published: January 2010

AbstractA Raman microscope using a total internal reflection (TIR) annular illumination geometry through a ZnSe solid immersion lens (SIL) is described. Spectra of a thin‐film sample of the transparent organic conductor poly(3,4‐ethylenedioxythiophene) poly(styrenesulfonate) (PEDOT:PSS) on a polyethylene terephthalate (PET) substrate are presented and compared with those from a conventional confocal Raman configuration. These spectra demonstrate a significant increase in surface selectivity upon the use of TIR illumination, as the decay length of the evanescent excitation field limits the depth of sample probed in this configuration. Spectral interference from the underlying PET substrate layer is thus greatly reduced. An increase in surface selectivity is also demonstrated for spectra acquired through the SIL with uniform illumination. Raman images of a micropatterned PEDOT:PSS film acquired with TIR illumination are also reported. Enhanced lateral resolution is realized in this configuration because of the immersion effect of the SIL, and the sampling depth is limited to 150 nm by the choice of illumination geometry. This results in analysis volumes on the order of tens of femtoliters, nearly two orders of magnitude smaller than typically achieved in conventional confocal Raman microscopes. This approach yields Raman spectra and images with surface selectivity significantly greater than can be achieved in confocal Raman, and provides a valuable tool for the microanalysis of thin surface films. Published in 2010 by John Wiley & Sons, Ltd.
Knowledge strategy: Its relationship to environmental dynamism and complexity in product development
Authors: Revilla Elena , Prieto Isabel Mª , Prado Beatriz Rodriguez 
Published: January 2010

AbstractFocusing on product development, this study extends the understanding of the environment‐strategy framework and investigates the relative effect of two environmental variables, dynamism and complexity, on the product development knowledge strategy. Adopting a knowledge‐based view, and assuming that the strategy's locus is knowledge creation (exploration) and knowledge application (exploitation), the study suggests that the development of a knowledge strategy is a managerial strategic choice that is related to the environment. The results of a survey on product development managers generally indicate that exploration and exploitation must be combined according to environmental factors by generating the alternative knowledge strategies of ambidexterity or punctuated equilibrium. Particularly, the study finds that in environments characterized by high levels of both dynamism and complexity product development efforts pursue and reinforce both explorative and exploitative activities through a knowledge strategy of ambidexterity. Though not perfectly supported, punctuated equilibrium seems to be a more plausible knowledge strategy in environmental contexts where either dynamism or complexity prevails. Copyright © 2010 John Wiley & Sons, Ltd.
Prolonged vastus lateralis denervation after botulinum toxin type A injection
Authors: Dunne John W , Singer Barbara J , Silbert Peter L , Singer Kevin P 
Published: January 2010

AbstractIntramuscular injection of botulinum toxin (BoNT) produces reversible blockade of neuromuscular transmission. In animal experimental models, recovery begins within four weeks and is usually complete by twelve weeks. We present evidence of prolonged denervation following BoNT injection of the vastus lateralis (VL) muscle to correct quadriceps muscle imbalance in patients with chronic anterior knee pain. Needle electromyography data were obtained from 10 subjects who had received a single BoNT treatment 5 to 19 months earlier as part of a clinical trial. Insertional and spontaneous activity, recruitment, and motor unit action potentials were examined. Clear differences between the injected and non‐injected VL muscles, which correlated with the time since injection, were identified in all subjects. All 10 subjects studied with needle EMG showed evidence of persisting denervation in the BoNT‐A injected VL muscle beyond the period of neuromotor recovery expected from animal experimental studies. © 2010 Movement Disorder Society
A novel splice site mutation in the SPG7 gene causing widespread fiber damage in homozygous and heterozygous subjects
Authors: Warnecke Tobias , Duning Thomas , Schirmacher Anja , Mohammadi Siawoosh , Schwindt Wolfram , Lohmann Hubertus , Dziewas Rainer , Deppe Michael , Ringelstein E. Bernd , Young Peter 
Published: January 2010

AbstractHereditary spastic paraplegias (HSP) are genetically and clinically heterogeneous neurodegenerative disorders. The purpose of this study was to assess the genotype and phenotype in a family with a complicated form of autosomal recessive hereditary spastic paraplegia (ARHSP). Neurological and neuropsychological evaluation, neurophysiologic studies, fiberoptic endoscopic evaluation of swallowing (FEES), neuroimaging analysis including diffusion tensor imaging (DTI), and mutation analysis of SPG4 and SPG7 gene were performed. The index case (mother) was affected by an adult‐onset form of complicated ARHSP due to the homozygous splice site mutation c.1552+1 G>T in the SPG7 gene. This mutation leads to an abnormally spliced mRNA lacking exon 11. Additional clinical features were bilateral ptosis and subtle deficits in executive function. All three asymptomatic daughters carried the sequence variation c.1552+1 G>T in heterozygous state. DTI of the mother revealed disturbance of white matter (WM) integrity in the left frontal lobe, the left corticospinal tract and both sides of the brainstem. DTI of the daughters showed subtle WM alteration in the frontal corpus callosum. The novel mutation is the first splice site mutation found in the SPG7 gene. It removes part of the AAA domain of paraplegin protein, probably leading to a loss‐of‐function of the paraplegin‐AFG3L2 complex in the mitochondrial inner membrane. The pattern of WM damage in the homozygote index case may be specific for SPG7‐HSP. The detection of cerebral WM alterations in the corpus callosum of asymptomatic heterozygote carriers confirms this brain region as the most prominent and early location of fiber damage in ARHSP. © 2010 Movement Disorder Society
The effects of physical activity on psychogenic movement disorders
Authors: Dallocchio Carlo , Arbasino Carla , Klersy Catherine , Marchioni Enrico 
Published: January 2010

AbstractPsychogenic movement disorders (PMD) are a diagnostic fascinating challenge in both neurologic and psychiatric setting. Many factors influence response to treatment, but few treatment strategies are available. Physical activity proves to be effective in the treatment of depression, anxiety, and other psychiatric disorders, but the effects of regular walking exercise on patients with PMD have never been investigated in a single‐blind study. Sixteen outpatients [13 women; mean age 33.0 years (range 22–51)] with primarily mild‐to‐moderate PMD completed a thrice‐weekly, 12‐weeks mild walking program. Assessments included DSM‐IV interview, the Psychogenic Movement Disorder Rating Scale (PMDRS), Beck Anxiety Inventory (BAI), Hamilton Depression Scale (HDS), V02 Max, and body mass index (BMI). Changes in total score on the PMDRS were the primary endpoint. A comparison of all measures taken at study onset and after completing the exercise program indicates statistically significant improvements. We observed a relevant improvement in 10 of 16 patients (62%). The mean difference for the primary outcome (PMDRS total) corresponded to about 70%. Compliance was good, and there were no adverse effects. This study provides preliminary evidence for regular low‐medium intensity exercise as a safe, adequate, and pleasing intervention for PMD. Furthermore, well‐designed studies appear justified to confirm these findings. © 2010 Movement Disorder Society
Pramipexole combined with levodopa improves motor function but reduces dyskinesia in MPTP‐treated common marmosets
Authors: Tayarani‐Binazir Kayhan A. , Jackson Michael J. , Rose Sarah , Olanow C. Warren , Jenner Peter 
Published: January 2010

AbstractReduced expression of dyskinesia is observed in levodopa‐primed MPTP‐treated common marmosets when dopamine agonists are used to replace levodopa. We now investigate whether a combination of the D‐2/D‐3 agonist pramipexole and levodopa also reduces dyskinesia intensity while maintaining the reversal of motor disability. Drug naïve, non‐dyskinetic MPTP‐treated common marmosets were treated daily for up to 62 days with levodopa (12.5 mg/kg plus carbidopa 12.5 mg/kg p.o. BID) or pramipexole (0.04–0.3 mg/kg BID) producing equivalent reversal of motor disability and increases in locomotor activity. Levodopa alone resulted in marked dyskinesia induction but little or no dyskinesia resulted from the administration of pramipexole. From day 36, some animals were treated with a combination of levodopa (3.125–6.25 mg/kg plus carbidopa 12.5 mg/kg p.o. BID) and pramipexole (0.1–0.2 mg/kg p.o. SID). This improved motor disability to a greater extent than occurred with levodopa alone. Importantly, while dyskinesia was greater than that produced by pramipexole alone, the combination resulted in less intense dyskinesia than produced by levodopa alone. These results suggest that pramipexole could be administered with a reduced dose of levodopa to minimize dyskinesia in Parkinson's disease while maintaining therapeutic efficacy. © 2010 Movement Disorder Society
What features improve the accuracy of the clinical diagnosis of progressive supranuclear palsy‐parkinsonism (PSP‐P)?
Authors: Williams David R , Lees Andrew J 
Published: January 2010

AbstractProgressive supranuclear palsy‐parkinsonism (PSP‐P) is a primary tauopathy characterised by neurofibrillary degeneration, which is frequently mistaken for Parkinson's disease (PD), multiple system atrophy (MSA), and vascular parkinsonism (VP) at presentation. The aim of this study was to identify particular clinical features (green flags) that may be helpful in differentiating PSP‐P from these other disorders. We identified 37 patients with PSP‐P from 726 patients archived at the Queen Square Brain Bank. Using a retrospective case notes review the clinical features were compared between the PSP‐P group and Lewy body associated parkinsonism (PD, n = 444 and dementia with Lewy bodies (DLB), n = 46), MSA (n = 90), and VP (n = 19), using the χ2‐test for proportions for a two‐by‐two contingency table. The sensitivity, specificity, and positive predictive values (PPV) and negative predictive values (NPV) were calculated for individual clinical features. A specificity of >0.85 or a PPV of >0.85 were considered reliable discriminators. No clinical features were predictive of PSP‐P, but late drug induced dyskinesias (specificity 0.92, PPV 0.99), late autonomic dysfunction (specificity 0.94, PPV 0.99) and any visual hallucinations (specificity 0.94, PPV 0.99) were better in distinguishing PD and PSP‐P than predicted using operational diagnostic criteria for PD. PSP‐P shares many clinical features with PD and DLB, MSA and VP, but visual hallucinations, drug induced dyskinesias and autonomic dysfunction are very uncommon and may be helpful exclusion criteria. © 2010 Movement Disorder Society