bluuebunny/crossref_metadata_2025_split huggingface dataset:
From diagnosis to health: a cross‐cultural interview study with immigrants from Somalia
Authors: Wallin Anne‐Marie , Ahlström Gerd 
Published: January 2010

Scand J Caring Sci; 2010; 24; 357–365

From diagnosis to health: a cross‐cultural interview study with immigrants from Somalia Objective:  Being diagnosed as having a chronic disease gives rise to emotions. Beliefs about health are culturally constructed and affect people’s decisions regarding treatment. No studies have been reported that focus on the health beliefs of immigrants of Somalian origin with diabetes and how these people experiences the diagnosis. Therefore the aim of the present study was to investigate how immigrants from Somalia living in Sweden experienced receiving the diagnosis and describe their beliefs about health.Method:  The sample consisted of 19 adults with diabetes born in Somalia and now living in Sweden who were interviewed with the aid of an interpreter. The interviews were subjected to qualitative content analysis.Results:  From the analysis of what the participants said about their experiences of the diagnosis there emerged three themes: ‘Existential brooding’, ‘Avoiding the diagnosis’ and ‘Accepting what is fated’. Three themes also emerged from the analysis of what they said about beliefs about health: ‘Health as absence of disease’, ‘Health as general well‐being’ and ‘Fated by a higher power’. A major finding was that women when they communicated their experiences regarding the diagnosis and health beliefs made more use of supernatural beliefs than men did. The participants, irrespective of gender, did not immediately respond with shock or other strong emotion when they received the diagnosis.Conclusions:  The study provides health‐care staff with knowledge concerning a minority group’s experiences of being diagnosed as having diabetes and their beliefs about health. The findings indicate that men and women differ in how they experiences the diagnosis and how they described their health beliefs. The quality improvement of health education and nursing for patients with diabetes calls for consideration of the variation of beliefs related to cultural background and gender.
Relevance of conserved lysine and arginine residues in transmembrane helices for the transport activity of organic anion transporting polypeptide 1B3
Authors: Glaeser H , Mandery K , Sticht H , Fromm MF , König J 
Published: January 2010

Background and purpose: Organic anion transporting polypeptide 1B3 (OATP1B3) (SLCO1B3) mediates the uptake of endogenous substrates (e.g. estrone‐3‐sulphate) and drugs (e.g. pravastatin) from blood into hepatocytes. Structure‐based modelling of OATP1B3 suggested that a pore with a positive electrostatic potential contributes to the transport mechanism. Therefore, we investigated the role of conserved positively charged amino acids for OATP1B3‐mediated uptake of sulphobromophthalein (BSP) and pravastatin.Experimental approach: Residues Lys28, Lys41 and Arg580 in OATP1B3 were substituted by alanine, arginine, glutamine, glycine or lysine. Using immunofluorescence, immunoblot analysis and cellular uptake assays, the effect of these mutations on protein expression and transport activity was investigated.Key results: Immunofluorescence revealed that all mutants were localized in the plasma membrane with partial intracellular retention of the Arg580>Ala and Arg580>Lys mutants. Lys41>Ala, Lys41>Gln, Lys41>Gly, Arg580>Gly and Arg580>Lys showed significantly reduced transport for BSP and pravastatin. Kinetic analyses of BSP transport revealed a significant reduction ofVmaxnormalized to cell surface protein expression for Lys41>Ala (wild type: 190 ± 8, Lys41>Ala:16 ± 4 pmol (mg protein)−1 min−1,P< 0.001), whereasVmaxof Lys41>Arg and Arg580>Lys (103 ± 8 and 123 ± 14 pmol (mg protein)−1 min−1,P> 0.05) did not change significantly. This suggests that the positive charges at positions 41 and 580 are important for transport activity of BSP. Structural modelling indicated that the positively charged side chain of Lys41 is flexible within the pore. The orientation of Arg580 is defined by adjacent residues Glu74 and Asn77, which was confirmed by kinetic analysis of Glu74>Ala.Conclusions and implications: We demonstrated that the conserved positively charged amino acids Lys41 and Arg580 are pivotal to the transport activity of OATP1B3.
The Abrasive Effect of a Porcelain and a Nickel–Chromium Alloy on the Wear of Human Enamel and the Influence of a Carbonated Beverage on the Rate of Wear
Authors: Joshi Nikhil , Patil Narendra P. , Patil Sanjayagouda B. 
Published: January 2010

Abstract Purpose: This study was conducted to determine the abrasive effect of a porcelain and an Ni–Cr alloy on the wear of human enamel, and the influence of a carbonated beverage on the rate of wear. Materials and Methods: Tooth specimens were prepared by embedding 48 freshly extracted mandibular first premolars in acrylic. Twenty‐four of these specimens were abraded against Ni–Cr, and the remaining 24 against porcelain in artificial saliva and carbonated beverage media, respectively (n = 12), on a specially designed abrasive testing machine at a constant load of 40 N with 6 mm amplitude for 15,000 cycles. The cusp heights of the tooth specimens were measured both before and after abrasion using a profile projector. The abraded cast specimens were subjected to profilometry for computing the surface roughness; the abrading media was subjected to atomic absorption spectrophotometry for analyzing Ni and Cr ion levels. Data obtained were statistically analyzed. Results: Porcelain specimens in a medium of carbonated beverage caused the highest wear of tooth specimens. The lowest wear of tooth specimens was Ni–Cr specimens in artificial saliva medium. Carbonated beverage caused significantly higher wear of tooth specimens when abraded against Ni–Cr and porcelain specimens than did artificial saliva. The mean quantitative surface roughness of porcelain specimens was significantly higher than that of Ni–Cr specimens, irrespective of the medium in which abrasion testing was conducted. There was no statistically significant difference between the concentrations of Ni ions released in artificial saliva and carbonated beverage media. Also, there was no statistically significant difference between the concentrations of Cr ions released in artificial saliva and carbonated beverage media. Conclusions: The wear of human enamel was significantly higher in the presence of carbonated beverage than artificial saliva and against porcelain when compared with Ni–Cr. The surface roughness of porcelain in the presence of carbonated beverage was found to be highest, and the release of Ni and Cr was not affected by carbonated beverage.
The potential impact of selective donor deferrals based on estimated blood volume on vasovagal reactions and donor deferral rates
Authors: Rios Jorge A. , Fang Junyong , Tu Yongling , Wright David J. , Spencer Bryan , Hillyer Christopher D. , Hillyer Krista L. , Eder Anne F. , Benjamin Richard J. 
Published: January 2010

BACKGROUND: Whole blood donation in the United States is restricted in volume to 10.5 mL/kg or less in an effort to prevent hypovolemic reactions, but still may exceed more than 15% of a donor's estimated blood volume (EBV). We analyzed the association of EBV with prefaint and systemic vasovagal reactions (SVRs) among whole blood donors and the potential impact of an EBV‐based deferral policy.STUDY DESIGN AND METHODS: Independent predictors for prefaint reactions and SVRs were assessed by multivariate logistic regression analysis on 591,177 unique donors participating in the Retrovirus Epidemiology Donor Study‐II study.RESULTS: Young age (16 years old odds ratio [OR], 3.70; 95% confidence interval [CI], 2.78‐4.94), low EBV (<3.5 L OR, 3.30; 95% CI, 2.57‐4.23), and first‐time donation status (OR, 2.33; 95% CI, 2.03‐2.67) were the strongest predictors for SVRs, with similar trends seen for prefaint reactions. Sex, height, race, blood center, and donation site were weakly associated predictors. A total of 5.6% of all donors had an EBV of less than 3.5 L and experienced 12.5% of all prefaint reactions and 14.5% of SVRs. The highest reaction rates were seen in donors less than 23 years old with an EBV of less than 3.5 L who comprised 2.7% of all donors, who were mostly female (99.9%), and who experienced 8.8% of prefaint reactions and 11.0% of SVRs.CONCLUSION: Young age, low EBV, and first‐time donation status are the major correlates of prefaint reactions and SVRs, suggesting that high school and college donors are at particular risk. Deferral of donors with low EBV who are less than 23 years old may offer a rational approach to protecting donors at greater risk of reactions without jeopardizing the adequacy of the blood supply.
Development and validation of a SYBR Green I–based real‐time polymerase chain reaction method for detection of haptoglobin gene deletion in clinical materials
Authors: Soejima Mikiko , Tsuchiya Yuji , Egashira Kouichi , Kawano Hiroyuki , Sagawa Kimitaka , Koda Yoshiro 
Published: January 2010

BACKGROUND: Anhaptoglobinemic patients run the risk of severe anaphylactic transfusion reaction because they produce serum haptoglobin (Hp) antibodies. Being homozygous for the Hp gene deletion (HPdel) is the only known cause of congenital anhaptoglobinemia, and clinical diagnosis of HPdel before transfusion is important to prevent anaphylactic shock. We recently developed a 5′‐nuclease (TaqMan) real‐time polymerase chain reaction (PCR) method.STUDY DESIGN AND METHODS: A SYBR Green I–based duplex real‐time PCR assay using two forward primers and a common reverse primer followed by melting curve analysis was developed to determine HPdel zygosity in a single tube. In addition, to obviate initial DNA extraction, we examined serially diluted blood samples as PCR templates.RESULTS: Allelic discrimination of HPdel yielded optimal results at blood sample dilutions of 1:64 to 1:1024. The results from 2231 blood samples were fully concordant with those obtained by the TaqMan‐based real‐time PCR method.CONCLUSION: The detection rate of the HPdel allele by the SYBR Green I–based method is comparable with that using the TaqMan‐based method. This method is readily applicable due to its low initial cost and analyzability using economical real‐time PCR machines and is suitable for high‐throughput analysis as an alternative method for allelic discrimination of HPdel.
Long‐term, retrospective evaluation (implant and patient‐centred outcome) of the two‐implants‐supported overdenture in the mandible. Part 1: survival rate
Authors: Vercruyssen M. , Marcelis K. , Coucke W. , Naert I. , Quirynen M. 
Published: January 2010

AbstractObjective: This retrospective analysis evaluated the long‐term outcome of two implants supporting an overdenture in the mandible, as well as the significance of some confounding factors (smoking, implant length, bone quality).Material and methods: All mandibular overdenture cases (n=495) treated during the past 25 years in our centre (with ≥5 years loading of the implants) were included in this study. General information (medical history, implant data, report on surgery) was retrieved from the patient's file. A large number of patients (n=248) were willing to visit the clinic for an additional follow‐up visit. For the others, information on implant survival was collected by phone (n=121), or contact was impossible (57 had died, three were hospitalized and 66 could not be reached). In the latter group, information was used, up to their last visit to the clinic. An implant was considered as surviving if it was still in function in the mouth, without clear adverse effects (pain, swelling, mobility). A failure was defined as early if it occurred within the window, insertion‐final prosthesis placement; afterwards, it was considered as late.Results: Most of the inserted implants (Brånemark type) were of the turned (machined) type (95.5%), the remainder was anodized (TiUnite). The anchoring system was either a bar (86.3%), ball attachments (11.7%) or magnets (1.6%), and only some patients changed from one to the other (0.4%). Kaplan–Meier analyses showed a survival rate of 95.5% after 20 years of loading. Factors that influenced the outcome included smoking (90% rate for smokers) and the surgical protocol (reduced survival rate for one‐stage‐placed implants). Implant length and bone quality had no impact.Conclusions: These results fully support the two‐implant overdenture concept in the mandible even in the long run. To cite this article:
Vercruyssen M, Marcelis K, Coucke W, Naert I, Quirynen M. Long‐term, retrospective evaluation (implant and patient‐centred outcome) of the two‐implants‐supported overdenture in the mandible. Part 1: survival rate. Clin. Oral Impl. Res. 21, 2010; 357–365. doi: 10.1111/j.1600‐0501.2009.01849.x
Immediate rehabilitation of the edentulous jaws with full fixed prostheses supported by four implants: interim results of a single cohort prospective study
Authors: Agliardi Enrico , Panigatti Stefano , Clericò Matteo , Villa Cristina , Malò Paulo 
Published: January 2010

AbstractObjectives: The purpose of this study was to prospectively evaluate the clinical and radiographic outcomes of immediately loaded full‐arch fixed prostheses supported by a combination of axially and non‐axially positioned implants in a large cohort of patients with completely edentulous jaws, up to 5 years of function.Materials and methods: One hundred and seventy‐three edentulous patients (80 males and 93 females) were enrolled according to specific selection criteria. Each patient received a full‐arch fixed prosthesis supported by two distal tilted implants and two anterior axially placed implants. The provisional functional acrylic prosthesis was delivered the same day as surgery in all cases. All cases were finalized 4–6 months later. The patients were scheduled for follow‐up at 6 and 12 months of function, and annually up to 5 years. At each follow‐up plaque and bleeding score was assessed and radiographic evaluation of marginal bone level was performed.Results: The overall follow‐up range was 4–59 months. A total of 154 immediately loaded prostheses (61 in the maxilla and 93 in the mandible) were in function for at least 1 year and were considered for the analysis. Four axially placed implants failed in the maxilla and one tilted implant in the mandible, all within 6 months of loading. No further implant failure occurred to date. Implant survival at 1 year was 98.36% and 99.73% for the maxilla and the mandible, respectively. Marginal bone loss at 1 year averaged 0.9±0.7 mm in the maxilla (204 implants) and 1.2±0.9 mm in the mandible (292 implants). No difference was found in marginal bone loss between axial and tilted implants. Plaque and bleeding scores progressively improved from 6 to 12 months. Fracture of the acrylic prosthesis occurred in 14% of total cases.Conclusions: The present preliminary results from a relatively large sample size suggest that the present technique can be considered a viable treatment option for the immediate rehabilitation of both mandible and maxilla. To cite this article:
Agliardi E, Panigatti S, Clericò M, Villa C, Malò P. Immediate rehabilitation of the edentulous jaws with full fixed prostheses supported by four implants: interim results of a single cohort prospective study. Clin. Oral Impl. Res. 21, 2010; 459–465. doi: 10.1111/j.1600‐0501.2009.01852.x
Immunoexpression of Cbfa‐1/Runx2 and VEGF in sinus lift procedures using bone substitutes in rabbits
Authors: De Souza Nunes Leandro Soeiro , De Oliveira Renato Victor , Holgado Leandro Andrade , Nary Filho Hugo , Ribeiro Daniel Araki , Matsumoto Mariza Akemi 
Published: January 2010

AbstractObjectives: To analyze and compare the expression of core binding factor‐1 (Cbfa‐1)/Runx2 and vascular endothelium growth factor (VEGF) in sinus lift procedures using bovine hydroxyapatite (HA) and β‐tricalcium phosphate (β‐TCP).Material and Methods: Twenty‐four male rabbits that had undergone bilateral sinus lift procedures were divided into three groups, according to the sinus filling material: Group 1: autogenous bone graft; Group 2: bovine HA; and Group 3: β‐TCP. All groups were sacrificed after 7, 14, 30 and 60 days, for microscopic, histomorphometry and immunohistochemistry analysis.Results: Microscopic analysis showed a similar bone repair pattern between the tested groups. New bone formation, soft and medular tissue, remaining material or particulate bone graft area were obtained by histomorphometric analysis. After 14 days, statistically significant differences in new bone formation were found between Group 1 (27.76±7.8) and Groups 2 (14.22±3.2) and 3 (11.1±7.7). After 30 days, statistically significant differences (P<0.05) were detected in bone formation between Groups 1 (31.39±36.5) and 2 (14.13±3.2). The last period showed improved bone formation in Group 2. Also, Group 2 showed higher Cbfa‐1/Runx2 immunoexpression when compared with Group 3. No remarkable differences were observed in VEGF immunoexpression among groups.Conclusion: Taken together, both biomaterials allowed bone tissue growth in a conductive pattern and did not interfere with bone remodeling in the late period, with a slight improvement in bone tissue formation when using HA, confirmed by marked expression of Cbfa‐1 at initial periods. To cite this article:
Nunes LSS, De Oliveira RV, Holgado LA, Nary Filho H, Ribeiro DA, Matsumoto MA. Immunoexpression of Cbfa‐1/Runx2 in sinus lift procedures using bone substitutes in rabbits. Clin. Oral Impl. Res. 21, 2010; 584–590. doi: 10.1111/j.1600‐0501.2009.01858.x
Screening for MPL mutations in essential thrombocythemia and primary myelofibrosis: normal Mpl expression and absence of constitutive STAT3 and STAT5 activation in MPLW515L‐positive platelets
Authors: Glembotsky Ana C. , Korin Laura , Lev Paola R. , Chazarreta Carlos D. , Marta Rosana F. , Molinas Felisa C. , Heller Paula G. 
Published: January 2010

AbstractObjectives:  To evaluate the frequency of MPL W515L, W515K and S505N mutations in essential thrombocythemia (ET) and primary myelofibrosis (PMF) and to determine whether MPLW515L leads to impaired Mpl expression, constitutive STAT3 and STAT5 activation and enhanced response to thrombopoietin (TPO).Methods:  Mutation detection was performed by allele‐specific PCR and sequencing. Platelet Mpl expression was evaluated by flow cytometry, immunoblotting and real‐time RT‐PCR. Activation of STAT3 and STAT5 before and after stimulation with increasing concentrations of TPO was studied by immunoblotting. Plasma TPO was measured by ELISA.Results:  MPLW515L was detected in 1 of 100 patients with ET and 1 of 11 with PMF. Platelets from the PMF patient showed 100% mutant allele, which was <50% in platelets from the ET patient, who also showed the mutation in granulocytes, monocytes and B cells. Mpl surface and total protein expression were normal, and TPO levels were mildly increased in the MPLW515L‐positive ET patient, while MPL transcripts did not differ from controls in both MPLW515L‐positive patients. Constitutive STAT3 and STAT5 phosphorylation was absent and dose response to TPO‐induced phosphorylation was not enhanced.Conclusions:  The low frequency of MPL mutations in this cohort is in agreement with previous studies. The finding of normal Mpl levels in MPLW515L‐positive platelets indicates this mutation does not lead to dysregulated Mpl expression, as frequently shown for myeloproliferative neoplasms. The lack of spontaneous STAT3 and STAT5 activation and the normal response to TPO is unexpected as MPLW515L leads to constitutive receptor activation and hypersensitivity to TPO in experimental models.
Increased expression of MAP2 inhibits melanoma cell proliferation, invasion and tumor growth in vitro and in vivo
Authors: Song Zhiqi , He Chun‐Di , Sun Changkai , Xu Yanni , Jin Xin , Zhang Yi , Xiao Ting , Wang Yakun , Lu Ping , Jiang Yi , Wei Huachen , Chen Hong‐Duo 
Published: January 2010

Please cite this paper as: Increased expression of MAP2 inhibits melanoma cell proliferation, invasion and tumor growth in vitro and in vivo. Experimental Dermatology 2010; 19: 958–964.Abstract:  Malignant melanoma (MM) is characterized by aggressive metastasis and high mortality rate. Microtubule‐associated proteins 2 (MAP2) is expressed abundantly in majority of melanocytic nevi and primary melanomas, but absent in metastatic melanomas. To determine whether MAP2 correlates with tumor progression of MM, we investigated the effects of MAP2 inhibition on the biological behaviour of metastatic melanoma in vitro and in vivo. Our results demonstrated that adenovirus‐mediated MAP2 induced apoptotic cell death and cell cycle arrest in metastatic human and mouse melanoma cell lines in vitro, and substantially inhibited the growth of melanomas in nude mice in vivo. In addition, intracellular expression of MAP2 was found to induce the morphologic alteration, suppress the migration and invasion and affect the assembly, stabilization and bundling of microtubules in melanoma cells. This is the first study that MAP2 expression significantly inhibits the growth of MM in vivo. Our results suggest that MAP2 may serve as a promising molecular target for therapy and chemoprevention of MM in humans.
Photodynamic therapy with topical methyl‐ and hexylaminolevulinate for prophylaxis and treatment of UV‐induced SCC in hairless mice
Authors: Togsverd‐Bo Katrine , Lerche Catharina M. , Poulsen Thomas , Wulf Hans Christian , Hædersdal Merete 
Published: January 2010

Please cite this paper as: Photodynamic therapy with topical methyl‐ and hexylaminolevulinate for prophylaxis and treatment of UV‐induced SCC in hairless mice. Experimental Dermatology 2010; 19: e166–e172.Abstract  Background:  Hexyl aminolevulinate (HAL) is a long‐chained 5‐aminolevulinic acid‐ester that has been proposed as a novel photosensitizing agent to methyl aminolevulinate (MAL) in topical photodynamic therapy (PDT). The more lipophilic HAL, may improve treatment outcome for non‐melanoma skin cancer. Objective: To compare the prophylactic and therapeutic effects of HAL‐ and MAL‐PDT for ultraviolet‐induced squamous cell carcinomas (SCCs) in hairless mice.Methods:  Mice (n = 249) were irradiated with solar UV‐radiation (UVR) until SCC occurred. Before any skin changes developed, two prophylactic PDT treatments were given, using creams of HAL (2%, 6%, 20%) or MAL (20%) followed by illumination (632 nm, Aktilite, Photocure). Two therapeutic PDT‐treatments were given by randomization to the first developed SCC of 1 mm. Primary end‐points were time to first SCC of 1 mm and complete SCC clearance. Secondary end‐points were time to SCC‐recurrence, PpIX fluorescence and skin reactions to PDT.Results:  The median time to first SCC was significantly longer for mice treated with prophylactic HAL‐PDT (2%, 6% and 20% HAL, 264 days) and MAL‐PDT (20% MAL, 269 days) than mice exposed to UVR (186 days) and UVR + placebo‐PDT (199 days) (P < 0.0001). The therapeutic efficacy of HAL‐ and MAL‐PDT showed cure rates of 23–61.5% (P = 0.11). Similar PpIX fluorescence intensity and severity of clinical reactions were seen for HAL‐ and MAL‐groups, although mice developed more intense hyper‐pigmentation when treated with 20% MAL‐PDT compared with 2% HAL‐PDT.Conclusions:  PDT with HAL (2%, 6% and 20%) and MAL (20%) is equally effective to prevent and treat UV‐induced SCC in hairless mice.