bluuebunny/crossref_metadata_2025_split huggingface dataset:
BRCA1/2 testing: uptake, phenocopies, and strategies to improve detection rates in initially negative families
Authors: Fischer C , Engel C , Sutter C , Zachariae S , Schmutzler R , Meindl A , Heidemann S , Grimm T , Goecke TO , Debatin I , Horn D , Wieacker P , Gadzicki D , Becker K , Schäfer D , Stock F , Voigtländer T ,   on behalf of the German Consortium for Hereditary Breast and Ovarian Cancer
Published: September 2011

Fischer C, Engel C, Sutter C, Zachariae S, Schmutzler R, Meindl A, Heidemann S, Grimm T, Goecke TO, Debatin I, Horn D, Wieacker P, Gadzicki D, Becker K, Schäfer D, Stock F, Voigtländer T, on behalf of the German Consortium for Hereditary Breast and Ovarian Cancer. BRCA1/2 testing: uptake, phenocopies, and strategies to improve detection rates in initially negative families.In families with clustering of breast and ovarian cancer, molecular testing of the major susceptibility genes BRCA1/2 helps to identify patients with disease mutations and healthy persons at high risk who can participate in targeted intervention programs. We investigated 5559 families from the German Consortium for Hereditary Breast and Ovarian Cancer included between 1997 and 2008 and treated under clinical routine conditions. In each family an index patient/person had been screened for deleterious mutations in BRCA1/2. Healthy relatives agreed to predictive testing in 888 of 1520 BRCA1/2 mutation‐positive families (58%). Of 2646 eligible unaffected first‐degree relatives 1143 decided to be tested (43%). In 325 families with BRCA1/2‐positive index patients one related BC/OC patient was tested and 39 (12.0%; 95% confidence interval: 8.7–16.0%) discrepant cases found. A second related individual was screened in 163 of 3388 (4.9%) families with BRCA1/2‐negative index patient and in eight families a BRCA1/2 mutation was found. In BRCA1/2 mutation‐positive families, BC/OC patients lacking the familial mutation have to be expected at a rather high rate. In families with BRCA1/2‐negative index patient we recommend a second screening if another patient with a high probability of carrying a BRCA1/2 mutation is available.
The effect of HLA disparity on clinical outcome after HLA‐haploidentical blood and marrow transplantation
Authors: Huo Ming‐Rui , Xu Lan‐Ping , D Li , Liu Dai‐Hong , Liu Kai‐Yan , Chen Huan , Han Wei , Chen Yu‐Hong , Y Wang , Wang Jing‐Zhi , Zhang Xiao‐Hui , Zhao Xiang‐Yu , Huang Xiao‐Jun 
Published: September 2011

Huo M‐R, Xu L‐P, Li D, Liu D‐H, Liu K‐Y, Chen H, Han W, Chen Y‐H, Wang Y, Wang J‐Z, Zhang X‐H, Zhao X‐Y, Huang X‐J. The effect of HLA disparity on clinical outcome after HLA‐haploidentical blood and marrow transplantation.  Clin Transplant 2011 DOI: 10.1111/j.1399‐0012.2011.01499.x.  © 2011 John Wiley & Sons A/S.Abstract:  The relative importance of various human leukocyte antigen (HLA) loci has not been established for unmanipulated HLA‐mismatched/haploidentical transplantation. To address this question, we analyzed the impact of HLA‐A, HLA‐B, HLA‐DRB1, HLA‐DRB3, HLA‐DRB4, and HLA‐DRB5 on the outcome of HLA‐haploidentical transplantation. Four hundred and eighty‐one donor–recipient pairs were fully typed before transplantation. In univariate analysis, HLA‐B mismatch not only demonstrated significant adverse effects on acute graft‐versus‐host disease (GVHD) and transplant‐related mortality but also was associated with reduced overall survival and leukemia‐free survival (LFS). In multivariate analysis, HLA‐B mismatch remained the independent risk factor for acute GVHD and transplant‐related mortality. The high risk of disease and the female donor were found to be significant factors for reduced overall survival and LFS. Furthermore, multiple mismatch of the HLA locus was found to have no synergistic adverse effect on outcomes. Our results suggest that prospective matching of patients and donors for HLA‐B antigen in the unshared HLA haplotype is warranted for HLA‐mismatched/haploidentical transplantation.
Impact of stable PGI2analog iloprost on early graft viability after liver transplantation: a pilot study
Authors: Bärthel Erik , Rauchfuß Falk , Hoyer Heike , Habrecht Olaf , Jandt Karin , Götz Max , Voigt Rigo , Heise Michael , Marx Gernot , Settmacher Utz 
Published: September 2011

Bärthel E, Rauchfuß F, Hoyer H, Habrecht O, Jandt K, Götz M, Voigt R, Heise M, Marx G, Settmacher U. Impact of stable PGI2analog iloprost on early graft viability after liver transplantation: a pilot study.  Clin Transplant 2012: 26: E38–E47.  © 2011 John Wiley & Sons A/S.Abstract: Background: Ischemia/reperfusion injury after liver transplantation (LT) may be associated with primary graft dysfunction (PDF) or non‐function. Prostaglandins were demonstrated to be beneficial in reducing ischemic injury by improving microcirculation and protecting endothelial cells. The aim of this study was to analyze the effect of the continuously administered prostaglandin I2analog iloprost on allograft function after LT.Methods: Eighty patients were prospectively randomized and assigned to two groups. Patients in the treatment group received iloprost for seven d after transplantation, and those in the control group did not. The primary end point was graft dysfunction.Results: The incidence of PDF was 20% (n = 8) in the control group and 5% (n = 2) in the treatment group, respectively (p = 0.087). Four patients in the control group underwent re‐transplantation for initial non‐function (INF). There was no evidence for INF in the treatment group. Iloprost was associated with improved allograft function. Clinical course and outcome were comparable.Conclusions: We suggest iloprost to be beneficial for early post‐transplant liver function. If the rate of PDF can be significantly reduced with this treatment concept, it should be analyzed in a larger number of patients (ISRCTN95672167).
Cryopreserved iliac artery allograft for primary arterial revascularization in adult liver transplantation
Authors: Mabrut Jean‐Yves , Abdullah Siraj Saadaldin , Rode Agnes , Bourgeot Jean‐Paul , Eljaafari Assia , Baulieux Jacques , Ducerf Christian 
Published: September 2011

Mabrut J‐Y, Abdullah SS, Rode A, Bourgeot J‐P, Eljaafari A, Baulieux J, Ducerf C. Cryopreserved iliac artery allograft for primary arterial revascularization in adult liver transplantation.  Clin Transplant 2012: 26: E12–E16.  © 2011 John Wiley & Sons A/S.Abstract:  Arterial allograft represents a material of choice for primary arterial revascularization in liver transplantation (LT) when interposition of a vascular conduit is required. In case of non‐availability of such graft, the use of cryopreserved vessels should be an interesting option. Three patients were grafted using a cryopreserved iliac artery allograft (CIAA) previously harvested and stored at −140°C in a tissue bank. An auxiliary partial LT was performed in one patient for acute liver failure. During follow‐up, an efficient regeneration of the native hemi‐liver was observed while atrophy of the auxiliary graft occurred, leading to functional portal vein and hepatic artery thrombosis at six and nine months, respectively. Two other patients presented with celiac trunk compression because of arcuate ligament without available arterial allograft in the donor. Late arterial thrombosis occurred at six months in one patient without impairment of graft function. The last patient was alive and symptom free 29 months after LT with a patent cryopreserved arterial conduit. Our preliminary results suggest that CIAA might represent an efficient solution as vessel interposition for primary arterial hepatic revascularization in LT setting when no other suitable graft is available. However, long‐term patency of CIAA remains questionable.
Extracorporeal life support as a bridge to high‐urgency heart transplantation
Authors: Barth Emeline , Durand Michel , Heylbroeck Christophe , Rossi‐Blancher Marine , Boignard Aude , Vanzetto Gerald , Albaladejo Pierre , Chavanon Olivier 
Published: September 2011

Barth E, Durand M, Heylbroeck C, Rossi‐Blancher M, Boignard A, Vanzetto G, Albaladejo P, Chavanon O. Extracorporeal life support as a bridge to high‐urgency heart transplantation.  Clin Transplant 2011 DOI: 10.1111/j.1399‐0012.2011.01525.x.  © 2011 John Wiley & Sons A/S.Abstract:  Extracorporeal life support (ECLS) represents an effective, emergent therapy for patients with end‐stage heart failure or cardiac arrest. However, ECLS is typically not used as a bridge to heart transplantation because of the limited duration of ECLS. In France, high‐urgency priority heart transplantation remains a possibility for transplant patients who are on ECLS. In this article, we present our experience with high‐urgency priority heart transplantation after ECLS. From July 2004 to December 2009, 242 patients underwent emergent ECLS. Heart transplantation was performed in eight of these patients. Time of ECLS was 6.3 ± 4.6 d. Before heart transplantation, all patients on ECLS had decreased organ dysfunctions and four were conscious. Despite frequent post‐operative complications, no death occurred during the first year after transplantation. In our experience, ECLS is a valid method of supporting patients awaiting high‐urgency heart transplantation and can be used as a short‐term bridge to heart transplantation.
Normal Feline Brain: Clinical Anatomy Using Magnetic Resonance Imaging
Authors: Mogicato G. , Conchou F. , Layssol‐Lamour C. , Raharison F. , Sautet J. 
Published: September 2011

With 6 figuresSummaryThe purpose of this study was to provide a clinical anatomy atlas of the feline brain using magnetic resonance imaging (MRI). Brains of twelve normal cats were imaged using a 1.5 T magnetic resonance unit and an inversion/recovery sequence (T1). Fourteen relevant MRI sections were chosen in transverse, dorsal, median and sagittal planes. Anatomic structures were identified and labelled using anatomical texts and Nomina Anatomica Veterinaria, sectioned specimen heads, and previously published articles. The MRI sections were stained according to the major embryological and anatomical subdivisions of the brain. The relevant anatomical structures seen on MRI will assist clinicians to better understand MR images and to relate this neuro‐anatomy to clinical signs.
Intact brown seaweed (Ascophyllum nodosum) in diets of weaned piglets: effects on performance, gut bacteria and morphology and plasma oxidative status
Authors: Michiels J. , Skrivanova E. , Missotten J. , Ovyn A. , Mrazek J. , De Smet S. , Dierick N. 
Published: September 2011

SummaryThe aim was to assess the effects of intact dried Ascophyllum nodosum seaweed on piglet performances, gut bacteria and function and plasma oxidative status. A total of 160 weaned piglets (21 days, 6.59 ± 0.91 kg) were allocated to four dietary treatments with eight pen replicates of five animals each for 28 days: a control diet; based on cereals, soybean meal and milk products, and three basal diets supplemented with either 2.5, 5.0 or 10.0 g dried seaweed per kg. At day 12/13 one piglet from each pen was sacrificed. Plasma samples were taken to determine parameters of oxidative status. Digesta were sampled for microbiological plate countings onto selective media and molecular analysis using PCR‐DGGE. Small intestinal tissue was taken for morphological and electro‐physiological determinations. Data were analysed by a linear model with treatment as fixed effect. A. nodosum supplementation had no effect on daily weight gain, nor did it alter feed conversion ratio. Plate countings failed to reveal differences among treatments. Dendograms prepared using PCR‐DGGE banding patterns did not indicate clustering of microbial profiles based on diet supplement. Plasma oxidative status and outcome of morphology and of electro‐physiological measurements from gut tissues were similar for all treatments. Thus, the addition of A. nodosum seaweed to well digestible diets did not enhance performances of piglets nor some gut health parameters and plasma oxidative status.
Recurrent selection for increased seed germination in sand bluestem (Andropogon hallii)
Authors: Springer Tim L. 
Published: September 2011

With 1 tableAbstractUnder field conditions, water is often inadequate for satisfactory seedling establishment (germination and emergence). This research was designed to improve the germination of sand bluestem (Andropogon hallii Hack.) populations ‘AB‐medium Syn‐0’ and ‘CD‐tall Syn‐0’ at low water potentials. Two cycles of recurrent selection were used to develop populations ‘AB‐medium Syn‐1’, ‘AB‐medium Syn‐2’, ‘CD‐tall Syn‐1’ and ‘CD‐tall Syn‐2’. Cycle 1 consisted of germinating 3500 open‐pollinated seeds of each Syn‐0 population in a −0.8 MPa D‐mannitol solution for seven days. All germinated seeds (approximately 250 of each population) were selected to create the Syn‐1 populations. Cycle 2 selection was similar to Cycle 1, except that Syn‐1 populations were used to create Syn‐2 populations. To test the effects of selection, four 50‐spikelet samples of the six populations were germinated at −0.8 MPa for seven days. Germination of Syn‐1 seeds was 6.7% higher than that of Syn‐0 seeds, and Syn‐2 seeds were 10.2% higher than that of Syn‐1 seeds. Thus, it was possible to select for increased seed germination of sand bluestem Syn‐0 populations at the water potential used herein.
Hypoadiponectinaemia in diabetes mellitus type 2: molecular mechanisms and clinical significance
Authors: Su Hui , Lau Wayne Bond , Ma Xin‐Liang 
Published: September 2011

Summary1. This review focuses on the regulatory mechanisms of adiponectin (APN) gene expression during physiologic conditions and both the clinical significance and underlying molecular mechanisms of hypoadiponectinaemia during pathologic conditions.2. Adiponectin is a versatile cardiovascular protective factor. It plays an important role in regulating insulin sensitivity and energy homeostasis, with anti‐inflammatory and anti‐atherosclerotic properties.3. Adiponectin gene expression is downregulated in both obesity and diabetes mellitus type 2. Hypoadiponectinaemia is an independent risk factor for coronary artery disease in type 2 diabetic patients.4. Exogenous supplementation of recombinant APN attenuates insulin resistance, improving metabolic disorders. Therefore, APN‐targeted pharmaceutical strategies increasing circulating APN levels may be therapeutic against type 2 diabetes.5. There is great value in elucidating the regulatory mechanisms of APN gene expression during physiologic and pathologic conditions. APN biosynthesis regulation includes transcriptional expression and post‐translational modification, oligomerization, and secretion. Under pathological conditions, including obesity and diabetes mellitus type 2, hypoxia, oxidative stress, and inflammation suppress APN mRNA levels and its secretion.
Effects of DA‐9701, a novel prokinetic agent, on gastric accommodation in conscious dogs
Authors: Kim Eun Ran , Min Byung‐Hoon , Lee Sung Ok , Lee Tae Ho , Son Miwon , Rhee Poong‐Lyul 
Published: September 2011

AbstractBackground and Aim:  DA‐9701, a novel prokinetic agent formulated with Pharbitis Semen and Corydalis Tuber, has strong prokinetic effects, and enhances gastric compliance in conscious dogs. In this study, the effects of DA‐9701 on gastric accommodation were studied in conscious dogs.Methods:  Beagle dogs with an implanted gastric cannula in the stomach were used in this study. After an overnight fast, the dogs received DA‐9701 orally, or served as a positive control that received sumatriptan or a negative control before ingestion of a meal. The basal and postprandial gastric volumes were monitored at a constant operating pressure using an electronic barostat. To investigate the long‐lasting effects on increased postprandial gastric volume, the area under the volume versus time curve (AUC) was calculated.Results:  DA‐9701 significantly increased the basal gastric volume compared to the negative controls (P < 0.05); the effects were comparable to sumatriptan. DA‐9701 and sumatriptan significantly increased gastric accommodation compared to the negative control (P < 0.05). In the negative control, the gastric volume reached the maximal volume 40 min after the meal, and then gradually decreased. However, with DA‐9701, the increased gastric volume remained significantly elevated for 60 min postprandially (P < 0.05). DA‐9701 significantly increased the value of AUC compared to the negative control; this was observed during both the early and late postprandial phases (P < 0.05).Conclusions:  A novel prokinetic agent, DA‐9701, improved gastric accommodation by increasing the postprandial gastric volume; these effects persisted for 60 min after a meal.
Effects of Meridian Massage on physical growth and infants' health as perceived by mothers
Authors: Cho Kyoul‐Ja , Ji Eun Sun , Lee Myung‐Hee 
Published: September 2011

AbstractBackground:  Meridian Massage is a traditional practice that manually stimulates the body's meridian system – the same network of vital energy channels used in acupuncture. The present study was to assess the effect of Meridian Massage on physical growth and infants' health as perceived by mothers.Methods:  A study was conducted in a community health center in Korea. A total of 169 healthy infant–mother dyads were assigned to the Meridian Massage group (n= 89) or the gentle touch massage group (n= 80), based on the mother's preference. All massages were conducted by the mothers for 15 min per session, one time daily over a course of 6 weeks. In each group, the infant's weights, heights, and the number of days with illness as perceived by mothers and related clinic visits were measured.Results:  Significant differences were observed in weight and height after 6 weeks between the Meridian group and the gentle touch massage group (P < 0.001). Infants in the Meridian Massage group showed a significantly different number of days with perceived clinic visits compared to those in the control group (P < 0.001).Conclusions:  Meridian Massage may facilitate physical growth and improve infants' health outcome as perceived by mothers. A randomized controlled trial is required to further explore the effects of Meridian Massage in early infancy.
Unexpected tumor progression after conization for carcinoma in situ of the uterine cervix
Authors: Omatsu Kohei , Takeshima Nobuhiro , Matoda Maki , Nomura Hidetaka , Umayahara Kenji , Sugiyama Yuko , Utsugi Kuniko , Tanaka Hiroko , Akiyama Futoshi , Takizawa Ken 
Published: September 2011

AbstractAim:  To determine the safety and usefulness of conization with an electrosurgical loop (the loop electrosurgical excision procedure [LEEP]) in young women with carcinoma in situ (CIS) of the uterine cervix.Methods:  A retrospective medical chart review of five patients with CIS initially treated with LEEP conization between 2002 and 2010 and who later experienced recurrence in the form of invasive or advanced disease was conducted.Results:  The median patient age at initial LEEP conization was 41 years (33–56 years). The margin status in conization specimens was positive in three of the five patients. The median time to detection of tumor progression was 50 months (17–58 months). Magnetic resonance imaging was effective in detecting tumor recurrence in all cases. In four patients, hysterectomy was performed to treat the recurrence; these four patients are currently alive and disease‐free. One patient with cervical lymph nodes and bone metastases was treated with chemotherapy alone; however, the patient died. In surgical specimens, tumor progression was observed extending toward the endometrium in all five patients and toward the vagina in two patients.Conclusion:  This study indicates that patients with CIS occasionally show unexpected tumor progression after conization and the progression could be life‐threatening. Recurrent disease often shows tumor progression toward the endometrium or vagina, leading to difficulties in its detection at periodic pelvic examinations.
Successfully treated case of epithelioid sarcoma of the vulva
Authors: Chiyoda Tatsuyuki , Ishikawa Mitsuya , Nakamura Masaru , Ogawa Mariko , Takamatsu Kiyoshi 
Published: September 2011

AbstractEpithelioid sarcoma of the vulva is an extremely rare and aggressive tumor. This tumor most commonly occurs on the labia majora in women of reproductive age. The molecular pathogenesis remains largely unknown. Only 20 cases of vulvar epithelioid sarcoma have been reported to date and more than half have had poor outcomes. We report a successfully treated case of vulvar epithelioid sarcoma in a 33‐year‐old woman. We performed a radical vulvectomy with flap reconstruction. Three years after surgery, the patient remains well, showing no evidence of recurrence. Early tissue diagnosis of vulvar epithelioid sarcoma is essential because this tumor can be clinically misdiagnosed as a benign lesion. Gynecologists should be aware of this rare tumor variant and carefully plan the treatment.
Birthweight placental weight ratio of appropriate‐for‐dates and light‐for‐dates infants in preterm delivery
Authors: Imada Shinya , Takagi Kimiyo , Kikuchi Akihiko , Ishikawa Kaori , Tamaru Shunsuke , Horikoshi Tsuguhiro , Ogiso Yoshifumi 
Published: September 2011

AbstractAim:  Although birthweight placental weight ratio (BPR) may be a promising indicator which reflects pathophysiology of fetal growth restriction (FGR), the standard of BPR changes throughout gestation in a Japanese population has not been established as far as we know. Therefore, we first examined BPR of appropriate‐for‐dates (AFD) infants in each gestational week in preterm deliveries. We then compared it with that in a group of light‐for‐dates (LFD) infants born from mothers with and without pregnancy‐induced hypertension (PIH).Material and Methods:  Placentas of a singleton pregnancy with 373 AFD and 110 LFD infants delivered from 22 to 36 weeks of gestation in our hospital during the period between September 2000 and December 2008 were included. We examined the placental weight and BPR of each gestational week in AFD and LFD groups. And the mean BPR and placental weight in the three groups (AFD: LFD with PIH: LFD without PIH) were compared according to gestational periods.Results:  The placental weight and BPR were significantly correlated to the gestational week both in AFD and LFD groups. We found that although the mean BPR in LFD‐PIH(−) group was significantly lower than those both in AFD group and in LFD‐PIH(+) group in 22–29 weeks, the mean BPR in 30–36 weeks was not statistically different among these three groups.Conclusion:  Our result in the AFD group may be useful as one of the standards of BPR changes throughout gestation in a Japanese population for future studies. We believe that BPR may be a clinically useful indicator which reflects pathophysiology of FGR.