bluuebunny/crossref_metadata_2025_split huggingface dataset:
Olanzapine augmentation of milnacipran for stage 2 treatment‐resistant major depression: an open study
Authors: Boku Shuken , Inoue Takeshi , Honma Hiroshi , Matsubara Shigehiro , Nakagawa Shin , Koyama Tsukasa 
Published: June 2011

ObjectiveOlanzapine augmentation of fluoxetine, a selective serotonin reuptake inhibitor, is an effective augmentation therapy for treatment‐resistant depression (TRD). However, studies of olanzapine augmentation of other antidepressants are few. We investigated the efficacy and safety of olanzapine augmentation of milnacipran, a serotonin–norepinephrine reuptake inhibitor, for TRD.MethodsThis study covered patients with stage 2 TRD, defined by Thase and Rush. Olanzapine was added to milnacipran, and its dosage was adjusted according to each patient. Previous treatments were continued, but no new treatments were allowed. Response was measured using Hamilton Depression Rating Scale (HAMD) and Clinical Global Impression at weeks 0, 1, 2, 3, 4, and 8.ResultsEleven patients aged 53.2 ± 24.0 years received olanzapine at 5.0 ± 1.9 mg/day with milnacipran. HAMD and Clinical Global Impression scores improved significantly from baseline to endpoint. This improvement occurred in week 1. At endpoint, seven of 11 (64%) were responders on HAMD (≥50% reduction). Four patients (36%) discontinued the trial because of no efficacy. No severe adverse effect occurred.ConclusionsOlanzapine augmentation of milnacipran for stage 2 TRD might be effective and well tolerated. However, our study is open label and uncontrolled. Therefore, a double‐blind controlled trial is necessary to confirm our results. Copyright © 2011 John Wiley & Sons, Ltd.
ABO blood group and breast cancer incidence and survival
Authors: Gates Margaret A. , Xu Mousheng , Chen Wendy Y. , Kraft Peter , Hankinson Susan E. , Wolpin Brian M. 
Published: June 2011

AbstractABO blood type has been associated with risk and survival for several malignancies; however, data for an association with breast cancer are inconsistent. Our study population consisted of Nurses' Health Study participants with self‐reported serologic blood type and/or ABO genotype. Using Cox proportional hazards regression, we examined the association between serologic blood type and incident breast cancer among 67,697 women, including 3,107 cases. In addition, we examined the association with ABO genotype in a nested case‐control study of 1,138 invasive breast cancer cases and 1,090 matched controls. Finally, we evaluated the association between serologic blood type and survival among 2,036 participants with breast cancer. No clear association was seen between serologic blood type or ABO genotype and risk of total breast cancer, invasive breast cancer or breast cancer subtypes. Compared to women with blood type O, the age‐adjusted incidence rate ratios for serologic blood type and total breast cancer were 1.06 (95% CI, 0.98–1.15) for type A, 1.06 (95% CI, 0.93–1.22) for AB and 1.08 (95% CI, 0.96–1.20) for B. In genetic analyses, odds ratios for invasive breast cancer were 1.05 (95% CI, 0.87–1.27) for A/O, 1.21 (95% CI, 0.86–1.69) for A/A, 0.84 (95% CI, 0.56–1.26) for A/B, 0.84 (95% CI, 0.63–1.13) for B/O and 1.17 (95% CI, 0.35–3.86) for B/B, compared to O/O. No significant association was noted between blood type and overall or breast cancer‐specific mortality. Our results suggest no association between ABO blood group and breast cancer risk or survival.
Labor market entry and earnings dynamics: Bayesian inference using mixtures‐of‐experts Markov chain clustering
Authors: Frühwirth‐Schnatter Sylvia , Pamminger Christoph , Weber Andrea , Winter‐Ebmer Rudolf 
Published: June 2011

SUMMARYThis paper analyzes patterns in the earnings development of young labor market entrants over their life cycle. We identify four distinctly different types of transition patterns between discrete earnings states in a large administrative dataset. Further, we investigate the effects of labor market conditions at the time of entry on the probability of belonging to each transition type. To estimate our statistical model we use a model‐based clustering approach. The statistical challenge in our application comes from the difficulty in extending distance‐based clustering approaches to the problem of identifying groups of similar time series in a panel of discrete‐valued time series. We use Markov chain clustering, which is an approach for clustering discrete‐valued time series obtained by observing a categorical variable with several states. This method is based on finite mixtures of first‐order time‐homogeneous Markov chain models. In order to analyze group membership we present an extension to this approach by formulating a probabilistic model for the latent group indicators within the Bayesian classification rule using a multinomial logit model. Copyright © 2011 John Wiley & Sons, Ltd.
Finite‐sample comparison of alternative methods for estimating dynamic panel data models
Authors: Akay Alpaslan 
Published: June 2011

SUMMARYThe Wooldridge method is based on a simple and novel strategy to deal with the initial values problem in nonlinear dynamic random‐effects panel data models. The characteristic of the method makes it very attractive in empirical applications. However, its finite sample performance and robustness are not fully known as of yet. In this paper we investigate the performance and robustness of this method in comparison with an ideal case in which the initial values are known constants; the worst scenario is based on an exogenous initial values assumption, and the Heckman's reduced‐form approximation method, which is widely used in the literature. The dynamic random‐effects probit and Tobit (type I) models are used as working examples. Various designs of the Monte Carlo experiments and two further empirical illustrations are provided. The results suggest that the Wooldridge method works very well only for the panels of moderately long duration (longer than 5–8 periods). Heckman's reduced‐form approximation is suggested for short panels (shorter than 5 periods). It is also found that all the methods tend to perform equally well for panels of long duration (longer than 15–20 periods). Copyright © 2011 John Wiley & Sons, Ltd.
GPM6B regulates osteoblast function and induction of mineralization by controlling cytoskeleton and matrix vesicle release
Authors: Drabek Ksenija , van de Peppel Jeroen , Eijken Marco , van Leeuwen Johannes PTM 
Published: June 2011

Abstract
Neuronal membrane glycoprotein gene (GPM6B) encodes a membrane glycoprotein that belongs to the proteolipid protein family. We identified GPM6B as a gene that is strongly upregulated during osteoblast differentiation. To investigate the role of GPM6B in the process of bone formation, we silenced GPM6B expression during osteogenic differentiation of human mesenchymal stem cells (hMSCs). GPM6B silencing in hMSCs resulted in reduced alkaline phosphate (ALP) activity along with reduced mineralization of extracellular matrix (ECM). Microarray expression analysis of GPM6B-depleted osteogenic hMSCs revealed significant changes in genes involved in cytoskeleton organization and biogenesis. Immunocytochemistry results confirm changes in the distribution of actin filaments, as well as the shape and size of focal adhesions on GPM6B silencing. Moreover, we demonstrated that production and release of ALP-positive matrix vesicles (MVs) were reduced. In conclusion, we identified GPM6B as a novel regulator of osteoblast function and bone formation. This finding demonstrates the significance of cytoskeleton organization for MV production and subsequent mineralization. © 2011 American Society for Bone and Mineral Research
High levels of β-catenin signaling reduce osteogenic differentiation of stem cells in inflammatory microenvironments through inhibition of the noncanonical Wnt pathway
Authors: Liu Na , Shi Songtao , Deng Manjing , Tang Liang , Zhang Guangjing , Liu Ning , Ding Bofu , Liu Wenjia , Liu Yali , Shi Haigang , Liu Luchuan , Jin Yan 
Published: June 2011

AbstractPeriodontal ligament stem cells (PDLSCs), a new population of mesenchymal stem cells (MSCs), have been isolated from the periodontal ligament (PDL). The capacity of multipotency and self-renewal makes them an excellent cell source for bone regeneration and repair. However, their bone-regeneration ability could be awakened in inflammatory microenvironments, which may be the result of changes in their differentiation potential. Recently, genetic evidences has shown that the Wnt pathway plays an important role in bone homeostasis. In this study we have determined the specific role of β-catenin in osteogenic differentiation of PDLSCs obtained from inflammatory microenvironments (P-PDLSCs). The inflammatory microenvironment, while inhibiting osteogenic differentiation potential, promotes proliferation of MSCs. A higher the level of β-catenin in P-PDLSCs than in H-PDLSCs (PDLSCs obtained from a healthy microenvironment) resulted in the same disparity in canonical Wnt signaling pathway activation between each cell type. Here we show that activation of β-catenin suppresses the noncanonical Wnt/Ca2+ pathway, leading to increased proliferation but reduced osteogenic differentiation of P-PDLSCs. Downregulation of the levels of β-catenin by treatment with dickkopf-1 (DKK-1) leads to activation of the noncanonical Wnt/Ca2+ pathway, which, in turn, results in the promotion of osteogenic differentiation in P-PDLSCs. Interestingly, β-catenin can affect both the canonical Wnt/β-catenin pathway and the noncanonical Wnt/Ca2+ pathway. Our data indicate that β-catenin plays a central role in regulating osteogenic differentiation of MSCs in inflammatory microenvironments. Given the important role of Wnt signaling in osteogenic differentiation, it is possible that agents that can modify this pathway may be of value in bone regeneration by MSCs in chronic inflammatory microenvironments. © 2011 American Society for Bone and Mineral Research
A novel graphical representation of protein sequences and its application
Authors: Liao Bo , Liao Benyou , Lu Xinguo , Cao Zhi 
Published: June 2011

AbstractOn the basis of information on the evolution of the 20 amino acids and their physiochemical characteristics, we propose a new two‐dimensional (2D) graphical representation of protein sequences in this article. By this representation method, we use 2D data to represent three‐dimensional information constructed by the amino acids' evolution index, the class information of amino acid based on physiochemical characteristics, and the order of the amino acids appearing in the protein sequences. Then, using discrete Fourier transform, the sequence signals with different lengths can be transformed to the frequency domain, in which the sequences are with the same length. A new method is used to analyze the protein sequence similarity and to predict the protein structural class. The experiments indicate that our method is effective and useful. © 2011 Wiley Periodicals, Inc. J Comput Chem, 2011
Single electron densities: A new tool to analyze molecular wavefunctions
Authors: Lüchow Arne , Petz René 
Published: June 2011

AbstractA new partitioning scheme for the electron density of a many‐electron wavefunction into single electron densities is proposed. These densities are based on the most probable arrangement of the electrons in an atom or molecule. Therefore, they contain information about the electron‐electron interaction and, most notably, the Fermi hole due to the antisymmetry of the many‐electron wavefunction. The single electron densities overlap and can be combined to electron pair distributions close to the qualitative electron pairs that represent, for instance, the basis of the valence shell electron pair repulsion model. Single electron analyses are presented for the water, ethane, and ethene molecules. The effect of electron correlation on the single electron and pair densities is investigated for the water molecule.
Theoretical modulation of the color of light emitted by firefly oxyluciferin
Authors: Silva Luís Pinto Da , Da Silva Joaquim C. G. Esteves 
Published: June 2011

AbstractOne of the major mysteries regarding firefly bioluminescence is its pH‐dependent multicolor variation. At basic pH, the emission is on the yellow–green region, whereas at acid pH, the light emission is observed on the red region of the visible spectrum. Theoretical calculations using density functional theory, molecular mechanics, and semiempirical methods were made to investigate the effect exerted by intermolecular forces on light emission, and their modulation by polarity, and the differences in the conformation of the active site at basic and acid pH. Red emission is achieved by the weakening of the interactions of the emitter with ionic and hydrophobic molecules, by the polarization of the benzothiazole microenvironment, by ionization of the enzyme‐emitter complex and by changes of the hydrogen bond network. Arg220, Glu346, Ala350, Leu344 and adenosine‐5′‐monophosphate have blue‐shifting effects, while His247, Phe249, Gly341, Thr253, and Ile288 exert a redshifting one. © 2011 Wiley Periodicals, Inc. J Comput Chem, 2011
Brownian dynamics simulations on CPU and GPU with BD_BOX
Authors: Długosz Maciej , Zieliński Paweł , Trylska Joanna 
Published: June 2011

AbstractThere has been growing interest in simulating biological processes under in vivo conditions due to recent advances in experimental techniques dedicated to study single particle behavior in crowded environments. We have developed a software package, BD_BOX, for multiscale Brownian dynamics simulations. BD_BOX can simulate either single molecules or multicomponent systems of diverse, interacting molecular species using flexible, coarse‐grained bead models. BD_BOX is written in C and employs modern computer architectures and technologies; these include MPI for distributed‐memory architectures, OpenMP for shared‐memory platforms, NVIDIA CUDA framework for GPGPU, and SSE vectorization for CPU. © 2011 Wiley Periodicals, Inc. J Comput Chem, 2011
A systematic review of measures of therapeutic engagement in psychosocial and psychological treatment
Authors: Tetley Amanda , Jinks Mary , Huband Nick , Howells Kevin 
Published: June 2011

AbstractThis article reports a systematic review of engagement measures for psychosocial therapy. MEDLINE, EMBASE, and PsycINFO databases were searched to identify English‐language studies (published 1980 to February 2010) that reported on an instrument/rating scale to measure engagement in psychosocial treatment for mental health difficulties. Forty‐seven studies were identified, reporting information on 40 measures of treatment engagement. Although our findings suggest that therapeutic engagement appears to be considered an important construct to assess, they also reveal that there is little consensus in the definition of engagement employed. Few measures are generalizable across treatment settings and clinical populations, and limited information is reported on the indices of reliability and validity. It is concluded that further work is required to develop adequate measures of therapeutic engagement. © 2011 Wiley Periodicals, Inc. J Clin Psychol 67:1–15, 2011.
A simple and efficient synthesis of [2H10]deuterated bromfenvinphos by the Perkow reaction
Authors: Huras Bogumiła , Konopski Leszek , Zakrzewski Jerzy 
Published: June 2011

Abstract(E,Z)‐2‐bromo‐1‐(2,4‐dichlorophenyl)vinyl bis[ethyl‐2H5] phosphate ([2H10]bromfenvinphos), a regiospecifically deuterium‐labelled pesticide, was synthesized in two steps starting from [2H6]ethanol, phosphorus trichloride and 2,4‐dichlorophenacylidene bromide, and fully characterized. The deuterated biologically active bromfenvinphos is an important compound for the advancement of environmental degradation testing and some mass spectrometric studies. Copyright © 2011 John Wiley & Sons, Ltd.
Serine–phosphoric acid cluster ions studied by electrospray ionization and tandem mass spectrometry
Authors: Kong Xianglei 
Published: June 2011

AbstractMore than 310 kinds of cluster ions of SmPnHkk+ are observed in a single ESI mass spectrum of a mixed solution of serine and phosphoric acid. Some typical cluster ions are selected, activated by collision in a FT ICR cell, and the dissociation pathways were deduced in detail. For large singly protonated ions, the collisions cause the ejection of subunits of serine or phosphoric acid subsequently producing the ions of S2P4H11+, which can be further dissociated by the loss of phosphoric acid molecules in turn and form the protonated serine dimer and monomer. However, for the doubly protonated ions, the dissociation pathways change from the loss of a protonated serine dimer for the ions of S7P9H22+ to the neutral loss of H3PO4 for the ions of S7P12H22+ or the neutral loss of serine or H3PO4 for the larger clusters, indicating the effect of cluster sizes on the process of dissociation. The structure of S2P4H11+ is suggested based on B3LYP/6‐31G(d,p) calculations. The diversity and structural orderliness of the hetero‐cluster ions are mainly attributed to the network of hydrogen bonds inside the cluster ions and the extraordinary amphotericity of the components. Copyright © 2011 John Wiley & Sons, Ltd.
Presence of galactosylated core fucose on N‐glycans in the planaria Dugesia japonica
Authors: Paschinger Katharina , Razzazi‐Fazeli Ebrahim , Furukawa Kiyoshi , Wilson Iain B. H. 
Published: June 2011

AbstractPlanarial species are of especial interest to biologists due to the phenomenon of pluripotency and, in comparison to other developmental processes, it can be hypothesised that glycan–lectin interactions may play a role. In order to examine the N‐glycans of one of these organisms, Dugesia japonica, peptide:N‐glycosidase A was employed and the released glycans were subject to pyridylamination, HPLC and mass spectrometric analysis. A range of oligomannosidic glycans was observed with a trimethylated Man5GlcNAc2 structure being the dominant species. Three glycans were also observed to contain deoxyhexose; in particular, a glycan with the composition Hex4HexNAc2Fuc1Me2 was revealed by exoglycosidase digestion, in combination with MS/MS, to contain a galactosylated core α1,6‐fucose residue, whereas this core modification was found to be capped with a methylhexose residue in the case of a Hex5HexNAc2Fuc1Me3 structure. This is the first report of these types of structures in a platyhelminth and indicates that the ‘GalFuc’ modification of N‐glycans is not just restricted to molluscs and nematodes. Copyright © 2011 John Wiley & Sons, Ltd.
High spatial resolution imaging mass spectrometry and classical histology on a single tissue section
Authors: Deutskens Fabian , Yang Junhai , Caprioli Richard M. 
Published: June 2011

AbstractThe work presented in this report describes and demonstrates a protocol for protein imaging analysis of biological tissue using MALDI IMS where histological staining and MS analysis are performed on the same tissue section. Spatial image resolution is shown at 35 µm for sagittal sections of the cerebellum from rat brain. Copyright © 2011 John Wiley & Sons, Ltd.
Nationwide surveillance of 18 respiratory viruses in patients with influenza‐like illnesses: A pilot feasibility study in the French Sentinel Network
Authors: Falchi Alessandra , Turbelin Clément , Andreoletti Laurent , Arena Christophe , Blanchon Thierry , Bonmarin Isabelle , Hanslik Thomas , Leruez‐Ville Marianne , De Lamballerie Xavier , Carrat Fabrice 
Published: June 2011

AbstractThe aim of the present study was to test the feasibility of integrating the diagnosis of 18 respiratory viruses into clinical surveillance of influenza‐like illness using a PCR‐DNA microarray detection assay. The study took place in the French Sentinel Network, a nationwide surveillance network of General Practitioners (GPs) representative of French GPs in terms age, location, and type of practice (urban/rural). Three virological laboratories also participated in the study. The study was planned for 5 weeks from January 25, 2010 to February 27, 2010. A subset of 150 Sentinel GPs, located in mainland France, was enrolled to collect clinical data and nasopharyngeal samples from every first patient of the week having a medical visit for influenza‐like illness defined as a sudden fever of 39°C or more with respiratory symptoms and myalgia. Sixty‐three GPs (42%) collected 103 samples while 87 GPs (58%) did not. GPs did not differ with respect to their age, gender, urban/rural distribution, or years of inscription in the Sentinel Network. Patients included were of a similar age and had similar vaccination characteristics, but were more frequently men than influenza‐like illness patients reported to the network during the study period. Sixty‐one viruses were detected from 56 of 96 (58%) interpretable samples. The respiratory viruses detected most frequently were metapneumovirus and respiratory syncytial virus. This study showed that virological diagnosis of 18 respiratory viruses can be combined with surveillance of clinical influenza‐like illness in general practice. Although feasibility has not been demonstrated yet, it will be evaluated over the winter of 2010–2011. J. Med. Virol. 83:1451–1457, 2011. © 2011 Wiley‐Liss, Inc.
Evaluation of a black‐footed ferret resource utilization function model
Authors: Eads David A. , Millspaugh Joshua J. , Biggins Dean E. , Jachowski David S. , Livieri Travis M. 
Published: June 2011

AbstractResource utilization function (RUF) models permit evaluation of potential habitat for endangered species; ideally such models should be evaluated before use in management decision‐making. We evaluated the predictive capabilities of a previously developed black‐footed ferret (Mustela nigripes) RUF. Using the population‐level RUF, generated from ferret observations at an adjacent yet distinct colony, we predicted the distribution of ferrets within a black‐tailed prairie dog (Cynomys ludovicianus) colony in the Conata Basin, South Dakota, USA. We evaluated model performance, using data collected during post‐breeding spotlight surveys (2007–2008) by assessing model agreement via weighted compositional analysis and count‐metrics. Compositional analysis of home range use and colony‐level availability, and core area use and home range availability, demonstrated ferret selection of the predicted Very high and High occurrence categories in 2007 and 2008. Simple count‐metrics corroborated these findings and suggested selection of the Very high category in 2007 and the Very high and High categories in 2008. Collectively, these results suggested that the RUF was useful in predicting occurrence and intensity of space use of ferrets at our study site, the 2 objectives of the RUF. Application of this validated RUF would increase the resolution of habitat evaluations, permitting prediction of the distribution of ferrets within distinct colonies. Additional model evaluation at other sites, on other black‐tailed prairie dog colonies of varying resource configuration and size, would increase understanding of influences upon model performance and the general utility of the RUF. © 2011 The Wildlife Society.