bluuebunny/crossref_metadata_2025_split huggingface dataset:
Co-application of titanium dioxide and hydroxyapatite nanoparticles modulated chromium and salinity stress via modifying physio-biochemical attributes in Solidago Canadensis L.
Authors: Nafchi Masomeh Alimohammadi , Kachoie Mehrdad Ataie , Ghodrati Leila 
Published: June 2024

Abstract

Climate change and human activity have led to an increase in salinity levels and the toxicity of chromium (Cr). One promising approach to modifying these stressors in plants is to use effective nanoparticles (NPs). It is unclear which well-known NPs are most effective in controlling salinity and Cr, as well as which one of salinity and Cr is more dangerous for plants. Although titanium dioxide nanoparticles (TiO2 NPs) have been used recently to decrease salt and Cr toxicity, it is unclear how TiO2 NPs compare to and function in conjunction with hydroxyapatite nanoparticles (HAP NPs). The objective of the current study was to determine the effects of foliar-applied TiO2 NPs (15 mg L− 1) and HAP NPs (250 mg L− 1) separately and in combination on growth, photosynthesis, water content, lipid peroxidation, antioxidant capacity, phenolic content, and essential oils (EOs) of Solidago Canadensis L. under salinity (100 mM NaCl) and Cr toxicity (100 mg kg− 1 soil). Salinity was more deleterious than Cr by decreasing plant weight, chlorophyll (Chl), relative water content (RWC), EO yield, and increasing malondialdehyde (MDA), electrolyte leakage (EL), superoxide dismutase (SOD) activity, and catalase (CAT) activity. The co-application of TiO2 and HAP NPs proved to be more successful. This was evidenced by the increased shoot weight (36%), root weight (29%), Chl a + b (23%), RWC (15%), total phenolic content (TPC, 34%), total flavonoid content (TFC, 28%), and EO yield (56%), as well as the decreased MDA (21%), EL (11%), SOD (22%) and CAT activity (38%) in salt-exposed plants. The results demonstrated the effective strategy of combining these NPs to modify abiotic stress.
Characterization of polystyrene-degrading bacteria isolated from plastic processing plants
Authors: Torkar Karmen Godič , Šunta Urška , Fink Rok , Modic Martina 
Published: June 2024

Abstract

In this study, the occurrence of the most common types of bacteria and fungi on the surfaces of production lines, raw materials and finished products in plastics processing plants was investigated. In addition, the ability of selected bacterial strains to form biofilms and to degrade PS surfaces was determined. The surfaces examined mainly contained aerobic mesophilic bacteria (42.0% of the samples), followed by moulds (30.0%) and representatives of the genera Bacillus (28.3%) and Staphylococcus (19.9%). Growth on the solid medium with polystyrene was confirmed in 63 (63.4%) of the 96 selected strains, but only 3 (3.0%) of them were able to form a clear zone around the colonies. The highest weight loss of polystyrene was observed in the presence of Bacillus strains. The degree of degradation after 30 and 60 days ranged from 0.12–0.78% and from 0.21–2.15%, respectively. The number of living cells in biofilms on surfaces was lowest for the Gram-negative strains Burkholderia and Moraxella and highest for most Bacillus and some Micrococcus strains. Fourier transform infrared spectroscopy revealed differences in the ratios of vibrational bands after exposure to bacterial strains, although no oxygen-containing functional groups were observed on the surface of polystyrene samples. The average contact angle values of polystyrene surfaces exposed to the tested bacterial strains decreased from 80.55 ± 0.39° to 73.64 ± 0.45° after 60 days of incubation. Some isolated bacterial strains from plastic production plants show the potential for more intensive degradation of such materials, which could have an impact on the quality and shelf life of plastic products.
Autoencoder-Based Data Clustering for Identifying Anomalous Repetitive Hand Movements, and Behavioral Transition Patterns in Children
Authors: Wedasingha Nushara Deshith , Samarasinghe Pradeepa , Senevirathe Lasantha , Papandrea Michela , Puiatti Alessandro 
Published: June 2024

Abstract

The analysis of repetitive hand movements and behavioral transition patterns holds particular significance in detecting atypical behaviors in early child development. Early recognition of these behaviors holds immense promise for timely interventions, which can profoundly impact a child's well-being and future prospects. However, the scarcity of specialized medical professionals and limited facilities has made detecting these behaviors and unique patterns challenging using traditional manual methods. This highlights the necessity for automated tools to identify anomalous repetitive hand movements and behavioral transition patterns in children. Our study aimed to develop an automated model for the early identification of anomalous repetitive hand movements and the detection of unique behavioral patterns. Utilizing autoencoders, self-similarity matrices, and unsupervised clustering algorithms, we analyzed skeleton and image-based features, repetition count, and frequency of repetitive child hand movements. This approach aimed to distinguish between typical and atypical repetitive hand movements of varying speeds, addressing data limitations through dimension reduction. Additionally, we aimed to categorize behaviors into clusters beyond binary classification. Through experimentation on three datasets (HMW, SSBD, ASD), our model effectively differentiated between typical and atypical hand movements, providing insights into behavioral transitional patterns. This aids the medical community in understanding the evolving behaviors in children. In conclusion, our research addresses the need for early detection of atypical behaviors through an automated model capable of discerning repetitive hand movement patterns. This innovation contributes to early intervention strategies for neurological conditions.
De novo transcriptome assembly and discovery of drought-responsive genes in eastern white spruce (Picea glauca)
Authors: Ribeyre Zoé , Depardieu Claire , Prunier Julien , Pelletier Gervais , Parent Geneviève J. , Mackay John , Droit Arnaud , Bousquet Jean , Nolet Philippe , Messier Christian 
Published: June 2024

Abstract

Background
Forests face an escalating threat from the increasing frequency of extreme drought events driven by climate change. To address this challenge, it is crucial to understand how widely distributed species of economic or ecological importance may respond to drought stress. Here, we used RNA-sequencing to investigate transcriptome responses at increasing levels of water stress in white spruce (Picea glauca (Moench) Voss), distributed across North America. We began by generating a transcriptome assembly emphasizing short-term drought stress at different developmental stages. We also analyzed differential gene expression at four time points over 22 days in a controlled drought stress experiment involving 2-year-old plants and three genetically unrelated clones.
Results
De novo transcriptome assembly and gene expression analysis revealed a total of 33,287 transcripts (18,934 annotated unique genes), with 4,425 unique drought-responsive genes. Many transcripts that had predicted functions associated with photosynthesis, cell wall organization, and water transport were down-regulated under drought conditions, while transcripts linked to abscisic acid response and defense response were up-regulated. Our study highlights a previously uncharacterized effect of drought stress on lipid metabolism genes in conifers and significant changes in the expression of several transcription factors, suggesting a regulatory response potentially linked to drought response or acclimation.
Conclusion
Our research represents a fundamental step in unraveling the molecular mechanisms underlying short-term drought responses in white spruce seedlings. In addition, it provides a valuable source of new genetic data that could contribute to genetic selection strategies aimed at enhancing the drought resistance and resilience of white spruce to changing climates.
Integration of hydro-chemical analysis and GIS for assessment of drinking water quality and health risks of filtration plants in Rawalpindi, Pakistan
Authors: Hussain Mushtaq , Farooqi Abida 
Published: June 2024

Abstract

The present study is about vast domain of water quality analysis, encompassing a comprehensive exploration of physical, chemical parameters and heavy metals i.e., Cadmium and lead, which determined the quality and composition of drinking water of 45 WASA filtration plants in Rawalpindi. All the physical parameters EC, TDS, salinity, and pH were calculated on site through field kits and chemical and ionic parameters were analyzed in the laboratory using various techniques of titrations and spectrophotometry. Water quality index was determined using all the parameters. Health risk assessment of heavy metals was done and Health Index for both children and adults were determined following the standard protocol of USEPA. For social and economic evaluation of plants interview was hold with the institute and a questionnaire survey was done to know the responses of public. Spatial distribution, variations of different parameters was shown in spatial maps made in ArcGIS by ESRI. Results showed elevated levels of lead and cadmium above the standards of WHO suggesting 21 % of plants are risky for public health. WQI results revealed that 40% of samples were of excellent quality and 13% showed very poor quality. Results of Ionic trends indicated high levels of Ca2+, HCO3- Mg2+ and Cl-. Cations show Ca2+ >Mg2+>Na+ while anions show HCO3->Cl->SO42->NO3>PO43- trend. From social survey it was found that there is contradiction between public perception and WASA operation. With overuse of water and growing population groundwater table is going down therefore water is supply twice a day for 4 hours. The findings illuminate the importance of continuous observance and systematic management of water systems. The study’s recommendations encompass a holistic monitoring approach, tailored treatment strategies, community empowerment, infrastructure upgrades, regulatory oversight, research-driven innovation, and interdisciplinary collaboration. Implementation of these strategies promises transformative outcomes, securing the purity and availability of water resources for a healthier and more prosperous future. In our role as custodians of this invaluable asset, it is our shared responsibility to safeguard its sanctity and sustain its vitality.
WITHDRAWN: Investigation of the anti-cancer drugs imatinib and thalidomide was conducted using analytical spectroscopy and molecular docking techniques
Authors: Ramana P. Venkata , Krishna Y. Rama , Mouli K. Chandra 
Published: June 2024

Abstract

Cancer stands as one of the most devastating illnesses in contemporary society, leading to a considerable number of fatalities annually. Effectively managing the disease has been a challenge, partly due to the diverse variants of the disease prevalent in different parts of the world. Despite these challenges, scientific advancements have led to the development of various drugs and diagnostic techniques tailored for specific cancers, offering partial solutions in the quest for a cure. The ongoing exploration of cancer's medical ramifications remains a captivating and vital area of interest, even in light of the extensive efforts expended in scientific research over the years. In this significant study, the research focuses on exploring specific vibrational patterns of Imatinib and Thalidomide through standard FT-IR spectroscopic studies and molecular docking computations. The investigation successfully pinpointed precise atomic-level interactions between the anti-cancer agent Imatinib and the target proteins, namely Tyrosine Kinase Sh2 Domain and Tyrosine-Protein Kinase ABL1, and the cancerous drug Thalidomide and Cereblon Isoform 4 protein. To understand the molecule's bioactivity and the transfer of charges between its outermost orbitals, the UV-Vis spectra of the drugs were scrutinized. Quantum mechanical energy-wavelength conversions were employed to assess the appropriate energy gaps. Moreover, the molecular docking analyses involving Imatinib and Thalidomide and the corresponding respective binding proteins provided crucial insights, including binding affinity, RMSD (Root Mean Square Deviation), types of interactions established as well as the unique pathways that the agent and receptors have developed. The revelations in comprehending the behaviour of anticancer agents represent invaluable contributions to the advancement of our understanding in the field. These findings not only enhance the efficacy of existing treatments but also play a pivotal role in steering the development of pioneering anticancer drugs. The significance of such discoveries cannot be overstated, as they contribute substantially to the ongoing progress in cancer research, offering promising avenues for the improvement of therapeutic interventions and the eventual development of more effective and targeted anticancer medications.
Successful salvage therapy of Ruxolitinib on interstitial pneumonia after long COVID or post COVID-19 syndrome with follicular lymphoma: two-case report and literature review
Authors: Zhu Tingting , Li Xin , Gao Shuquan , Cui Rui , Wang Jia , Deng Qi 
Published: June 2024

Abstract

Immunocompromised caused by B lymphocyte deficiency and hypogammaglobulinemia after anti-CD19 Chimeric antigen receptor (CAR) T cell therapy for patients with relapsed/refractory (R/R) follicular lymphoma (FL) are high risks of severe COVID-19 infection. In our study, two patients with refractory FL had persistence of COVID-19 infection after their anti-CD19-CAR T cell therapy. They were diagnosed with Post COVID-19 syndrome or Long COVID-19 with interstitial inflammation and persistent hypoxemia. They received Molnupiravir and/or Paxlovid, methylprednisolone therapy when their interleukin (IL)-6 was is at a high level. There was no response in interstitial inflammation, persistent hypoxemia and persistent positive expression of SARS-CoV-2 to the therapy above, but the level of IL-6 was decreased after these therapies. These two patients subsequently received low-dose of Ruxolitinib (5mg, twice a day) as a salvage therapy in combination with a gradually reduced dosage of methylprednisolone. One to two months of Ruxolitinib therapy, the persistent hypoxemia was relieved and the interstitial inflammation was significantly absorbed. At the same time, the SARS-CoV-2 detection was found to be negative. Even if SARS-CoV-2 was positive again, the interstitial pneumonia did not progress again and the symptoms such as dyspnea did not develop again. Ruxolitinib might be a safe and effective alternative salvage therapy for COVID-19 infection patients with interstitial inflammation and persistent hypoxemia who had no response to corticosteroid therapy.
Effect of Domestic Cooking on Total and Individual Carotenoid Profiles of Potato
Authors: Xu Jian , Zeng Fankui 
Published: June 2024

Abstract

Total and individual carotenoid concentrations were determined in raw potato tubers of 6 varieties including Kexin No. 1, Xisen No. 6, Atlantic, Xisen No. 5, Shepody, and Favorita. The relationship between color CIE L*a*b* and total carotenoid concentration, and the effect of domestic thermal treatments on the total and individual carotenoid concentrations were also investigated. The total carotenoid content in the 6 potato varieties ranged from 96.48 to 875.08 µg/100 g FW. The main carotenoids were lutein, zeaxanthin, violaxanthin, and antheraxanthin. The specific composition depended on the variety, β-carotene was not detected in all tested six potato varieties. The color value of coordinate b* was positively and significantly correlated with total carotenoid content. Boiling had the smallest effect on the decrease of total and individual carotenoids, while microwave treatment resulted with 52.67% in the highest loss of the total carotenoid in Favorita. As for the individual carotenoids, violaxanthin followed by antheraxanthin were the most heavily affected by cooking. All four thermal treatments, baking, boiling, frying and microwave decreased the carotenoid contents, boiling is recommended as a preferred method associated the lowest loss rate of total and individual carotenoids.
Epidemiology Forecasting Analysis of Dengue Cases with Seasonal Autoregressive Integrated Moving Average in Davao City, Philippines
Authors: Plaza George Carr , Servillon Rvin John , Alempang Hasmin , Boquia Ynan Bab Shariff , Gonzales Kaecey Airen , Japitan Kyoko , Parba Shaina Grace , Hinay Alfredo 
Published: June 2024

Abstract

Dengue is a mosquito-borne illness and an important public health problem in the Philippines, with the highest number of dengue cases in Southeast Asia. The Philippine government has created a dengue surveillance map to track illness transmission; however, model-based methods for forecasting and detecting dengue have not been imposed and are significantly sought-after throughout the country. In this study, a suitable time-based model for predicting dengue cases was used. Using the seasonal autoregressive integrated moving average (SARIMA) model, this study developed a forecasting model to predict dengue cases in Davao City, Philippines from to 2020–2025 based on data gathered from the Department of Health Region XI from years 2013–2019. The root mean square error (RMSE) and mean absolute percentage error (MAPE) were used as criteria for choosing an accurate forecasting model, and the QGIS version 3.16. was used for mapping. The results showed a monthly variation in the spike of dengue cases, peaking during July and August in both past and forecasted cases. The combined forecast model was found to be the best-fitting model for predicting future dengue incidences in the administrative districts of Davao City for the years 2020–2025.
A Comprehensive Risk Analysis of Physicochemical Parameters in Sharda Canal's Surface Water, Uttar Pradesh, India
Authors: Verma Abhishek , Patel Prabhat Kumar , Singh Asit 
Published: June 2024

Abstract

The objective of the research was to assess the state of the Sharda Canal, a critical irrigation and household water source in Uttar Pradesh, India. More specifically, the analysis applied the Water Quality Index methodology. A total of five survey sites were selected along the 258.80 km of the Sharda Kheri Branch, chosen based on the land use type and potential source of pollution. The analysis examined chemical concentration and dissolved solids in terms of pH, DO, COD, BOD, Nitrates, Sulphates, TDS, TSS, hardness, alkalinity, calcium, and magnesium as the most relevant parameters. WQI was calculated using the weighted arithmetic index method. The results show a significant difference in the pollutant load between the locations. The water quality index at the Sharda Barrage in Lakhimpur Kheri was 110.60, and at the Bakshi ka talab distributary in Lucknow, the water quality index was 124.92. The obtained high values of TDS, COD, sulfates, and nitrates reflect the probable existence of contaminants that could cause catastrophic impacts on the regional water quality and aquatic ecology. In conclusion, the results of this evaluation draw clear lines and demonstrate a genuine integrated action is warranted to curb the quick decay of water quality in the Sharda Canal. As a result, the WQI, an integrated physicochemical appraisal tool, has been used to achieve a concise understanding of the water quality variables at work in the Sharda Canal. Possible measures to improve the situation may involve expanding industrial and agricultural regulations and practices, enhancing treatment plants’ efficiency, raising public awareness, and decreasing pollution sources.
Bispectral index monitoring offers promise as an adjunct to ciprofol sedation in patients undergoing painless gastrointestinal endoscopy
Authors: Cao Xinyu , Xu Miaomiao , Zhou Jiejie , Xiong Jingwei , Zhang Jie , Zhang Lidong , Hui Kangli 
Published: June 2024

Abstract

Background: This study was devised to assess the efficacy and utility of bispectral index (BIS) monitoring as an adjunct to ciprofol-based sedation in patients undergoing gastrointestinal endoscopy, based on the hypothesis that BIS monitoring would reduce ciprofol consumption and shorten the time required for recovery.
Methods: A total of 140 patients scheduled to undergo painless gastrointestinal endoscopy procedures were enrolled in this study. Patients were intraoperatively randomized into BIS or control groups. The dose of ciprofol required was the primary study endpoint, while secondary endpoints included intraoperative SpO2 changes, induction time, the duration of endoscopic examination, time to awakening, time to post-anesthesia care unit (PACU) discharge, adverse event incidence, and satisfaction levels for both patients and endoscopists.
Results: The demographic characteristics of both enrolled cohorts were comparable. A significantly lower level of ciprofol consumption was observed for the BIS group relative to the control group (62.27±14.38 mg vs. 66.92±11.66 mg, respectively, P=0.037). The BIS group also presented with a shorter time to awakening (P<0.001) and PACU discharge (P<0.001), together with greater endoscopist satisfaction relative to the control group (P=0.029). The incidence of unfavorable outcomes was similar in both groups (P >0.05 for all).
Conclusions: These results show that BIS monitoring, when employed as an adjunct to outpatient ciprofol sedation, was associated with a significantly lower level of ciprofol consumption during gastrointestinal endoscopy procedures, together with reductions in the time to patient awakening and PACU discharge. This monitoring strategy may also lead to greater satisfaction among endoscopists.
Trial registration: This trial has been submitted to the Chinese Clinical Trial Registry (registration number: ChiCTR2300069868, registration date: 28/03/2023).
Navigating the new normal: rethinking conservation strategies in the climate-impacted and highly invaded eastern Mediterranean
Authors: Katsanevakis Stelios , Belmaker Jonathan , Rilov Gil , Yeruham Erez , Konstantinidis Andreas , Papazekou Maria , Giakoumi Sylvaine , Mazaris Antonios D. 
Published: June 2024

Abstract

We aimed to assess the social-ecological roles of marine alien species in the eastern Mediterranean Sea – a global hotspot of bioinvasions and local extinctions – and their potential contribution to reaching Targets 1, 3, 10, and 11 of the Kunming-Montreal Global Biodiversity Framework (GBF). We employed a comprehensive methodology, incorporating a literature review, fish surveys, and a consumer behavior questionnaire. Furthermore, we applied spatial conservation prioritization to identify reef fish conservation areas, both with and without considering marine alien species. Our findings reveal the dual role of alien species, with both adverse and beneficial effects on biodiversity, ecosystem functions, and services. Based on our analyses, we advocate for a paradigm shift in conservation, moving beyond the traditional focus solely on native biodiversity to include alien species as contributors to ecosystem functionality and services, especially in regions where native biodiversity is rapidly declining due to ocean warming. Our results highlight the importance of integrating alien species considerations into conservation planning to achieve GBF targets 1 and 3, emphasizing their potential to maintain and enhance ecosystem services, such as food provision through fisheries (GBF Targets 10 and 11), amidst rapid climate change. We present a framework for incorporating both the positive and negative aspects of alien species into Systematic Conservation Planning to guide conservation investments efficiently. By adopting a pragmatic, holistic approach to conservation and recognizing the diverse impacts of alien species, decision-makers could more effectively achieve GBF Targets 1, 3, 10, and 11 in the eastern Mediterranean.