bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discrimination of probiotic Lactobacillus strains for poultry by repetitive sequenced‐based PCR fingerprinting
Authors: Lee Chin Mei , Sieo Chin Chin , Cheah Yoke‐Kqueen , Abdullah Norhani , Ho Yin Wan 
Published: September 2011

AbstractBACKGROUND: Four repetitive element sequence‐based polymerase chain reaction (rep‐PCR) methods, namely repetitive extragenic palindromic PCR (REP‐PCR), enterobacterial repetitive intergenic consensus PCR (ERIC‐PCR), polytrinucleotide (GTG)5‐PCR and BOX‐PCR, were evaluated for the molecular differentiation of 12 probiotic Lactobacillus strains previously isolated from the gastrointestinal tract of chickens and used as a multistrain probiotic. This study represents the first analysis of the comparative efficacy of these four rep‐PCR methods and their combination (composite rep‐PCR) in the molecular typing of Lactobacillus strains based on a discriminatory index (D).RESULTS: Species‐specific and strain‐specific profiles were observed from rep‐PCR. From the numerical analysis of composite rep‐PCR, BOX‐PCR, (GTG)5‐PCR, REP‐PCR and ERIC‐PCR, D values of 0.9118, 0.9044, 0.8897, 0.8750 and 0.8529 respectively were obtained. Composite rep‐PCR analysis was the most discriminative method, with eight Lactobacillus strains, namely L. brevis ATCC 14869T, L. reuteri C 10, L. reuteri ATCC 23272T, L. gallinarum ATCC 33199T, L. salivarius ATCC 11741T, L. salivarius I 24, L. panis JCM 11053T and L. panis C 17, being differentiated at the strain level.CONCLUSION: Composite rep‐PCR analysis is potentially a useful fingerprinting method to discriminate probiotic Lactobacillus strains isolated from the gastrointestinal tract of chickens. Copyright © 2011 Society of Chemical Industry
Phenolic characterisation of red wines from different grape varieties cultivated in Mendoza province (Argentina)
Authors: Fanzone Martín , Zamora Fernando , Jofré Viviana , Assof Mariela , Gómez‐Cordovés Carmen , Peña‐Neira Álvaro 
Published: September 2011

AbstractBACKGROUND: Knowledge of the chemical composition of wine and its association with the grape variety/cultivar is of paramount importance in oenology and a necessary tool for marketing. Phenolic compounds are very important quality parameters of wines because of their impact on colour, taste and health properties. The aim of the present work was to study and describe the non‐flavonoid and flavonoid composition of wines from the principal red grape varieties cultivated in Mendoza (Argentina).RESULTS: Sixty phenolic compounds, including phenolic acids/derivatives, stilbenes, anthocyanins, flavanols, flavonols and dihydroflavonols, were identified and quantified using high‐performance liquid chromatography with diode array detection coupled with electrospray ionisation mass spectrometry (HPLC‐DAD/ESI‐MS). Marked quantitative differences could be seen in the phenolic profile among varieties, especially in stilbenes, acylated anthocyanins and other flavonoids.CONCLUSION: The polyphenolic content of Malbec wines was higher compared with the other red varieties. Dihydroflavonols represent a significant finding from the chemotaxonomic point of view, especially for Malbec variety. This is the first report on the individual phenolic composition of red wines from Mendoza (Argentina) and suggests that anthocyanins, flavanols and phenolic acids exert a great influence on cultivar‐based differentiation. Copyright © 2011 Society of Chemical Industry
Micromachining using high‐power picosecond lasers
Authors: Engelhardt Uli , Hildenhagen Jens , Dickmann Klaus 
Published: September 2011

AbstractFor the micromachining of temperature‐sensitive and brittle‐rigid components ultra short pulsed lasers are being used more and more. Due to the short pulse durations in the femto‐ and pico second range such systems with only a few Watt of average power have high pulsed peak powers of an order of 10 MW. This allows machining a wide range of materials including transparent materials via non‐linear absorption, enabled by the high intensity in the focus of radiation. The intensity can reach values of several TW/cm2. This leads to the immediate evaporation of the material, ideally, without melt and burr.
MAKING LAND MANAGEMENT MORE SUSTAINABLE: EXPERIENCE IMPLEMENTING A NEW METHODOLOGICAL FRAMEWORK IN BOTSWANA
Authors: Perkins J. , Reed M. , Akanyang L. , Atlhopheng J. , Chanda R. , Magole L. , Mphinyane W. , Mulale K. , Sebego R. , Fleskens L. , Irvine B. , Kirkby M. 
Published: September 2011

ABSTRACTIt is increasingly recognised that tackling land degradation through more sustainable land management depends on incorporating multiple perspectives by using a variety of methods at multiple scales, including the perspectives of those who manage and/or use the land. This paper reports experience implementing a previously proposed methodological framework that is designed to facilitate knowledge sharing between researchers and stakeholders about land degradation severity and extent, and sustainable land management options. Empirical findings are presented from the Botswana site of the EU‐funded Desertification Mitigation and Remediation of Land project. The paper reflects upon the challenges and benefits of the proposed framework and identifies a number of benefits, notably related to insights arising from the integration of local and scientific knowledge, and the ownership of the sustainable land management strategies that emerged from the process. However, implementing the framework was not without challenges, and levels of poverty and formal education may limit the implementation of the framework in some developing world contexts. Copyright © 2011 John Wiley & Sons, Ltd.
3D THz metamaterials from micro/nanomanufacturing
Authors: Moser H.O. , Rockstuhl C. 
Published: September 2011

AbstractMetamaterials are engineered composite materials offering unprecedented control of wave propagation. Despite their complexity, effective properties can frequently be extracted by conceptualizing them as homogeneous and isotropic media with dispersive electric permittivity and magnetic permeability. For an ideal isotropic medium, strong dispersion in these properties causes wave and field vectors to form a left‐handed (E,H,k)‐frame involving backward waves, and offering control of quantities like the refractive index which may become negative. Experimental evidence exists from microwaves to the visible. Applications include sub‐wavelength‐resolution imaging, invisibility cloaking, plasmonics‐based lasers, metananocircuits, and omnidirectional absorbers. As the engineered sub‐structures must be smaller than their design wavelength, micro/nanomanufacturing is exploited from primary pattern generation over lithography to templating and molecular beam epitaxy. 3D metamaterials have been made by stacking of layers, multilayer structuring, and 3D primary pattern generation. Theory shows that full properties may build up over one or a very few layers.
A high‐fat diet increases angiogenesis, solid tumor growth, and lung metastasis of CT26 colon cancer cells in obesity‐resistant BALB/c mice
Authors: Park Heesook , Kim Minhee , Kwon Gyoo Taik , Lim Do Young , Yu Rina , Sung Mi‐Kyung , Lee Ki Won , Daily James W. , Park Jung Han Yoon 
Published: September 2011

AbstractWe evaluated whether high‐fat diet (HFD), in the absence of increased calorie intake, increases colon cancer growth and metastasis. Four‐week‐old male BALB/c mice were fed on an HFD (60 kcal% fat) or control diet (10 kcal% fat) for 16 wk, after which CT26 colon cancer cells were subcutaneously injected into the right flank. Solid tumor growth and the number and volume of tumor nodules in the lung were increased markedly in the HFD group with only a slight increase in body weight (5.9%). HFD feeding increased tumor tissue levels of Ki67, cyclin A, cyclin D1, CDK2, Bcl‐xL, and Bcl‐2; reduced p53 levels and TUNEL‐positive apoptotic cells; increased the levels of CD45, CD68, CD31, VEGF, P‐VEGF receptor‐2, iNOS, and COX‐2 as well as hemoglobin content; and increased the levels of HIF‐1α, P‐STAT3‐Y705, P‐STAT3‐S727, P‐IκB‐α, P‐p65, p65, P‐c‐Jun, P‐Akt, P‐ERK1/2, P‐p38, and P‐SAPK/JNK. HFD feeding increased the serum levels of EGF, insulin, IGF‐I, IFN‐γ, leptin, RANTES, MCP‐1, IL‐1ra, and SDF‐1α and media conditioned by epididymal fat tissue explants from HFD‐fed mice caused an increase in microvessel outgrowth from the mouse aorta and tube formation of human umbilical vein endothelial cells. These results indicate that the chronic consumption of an HFD increases colon cancer cell proliferation, tumor angiogenesis, and lung metastasis in mice in the absence of discernible weight gain. HFD feeding increases the levels of growth factors which activate transcription factors, thereby inducing the expression of many genes involved in the stimulation of inflammation, angiogenesis, and cellular proliferation. © 2011 Wiley Periodicals, Inc.
Basal ganglia atrophy in prodromal Huntington's disease is detectable over one year using automated segmentation
Authors: Majid D.S. Adnan , Aron Adam R. , Thompson Wesley , Sheldon Sarah , Hamza Samar , Stoffers Diederick , Holland Dominic , Goldstein Jody , Corey‐Bloom Jody , Dale Anders M. 
Published: September 2011

AbstractFuture clinical trials of neuroprotection in prodromal Huntington's (known as preHD) will require sensitive in vivo imaging biomarkers to track disease progression over the shortest period. Since basal ganglia atrophy is the most prominent structural characteristic of Huntington's pathology, systematic assessment of longitudinal subcortical atrophy holds great potential for future biomarker development. We studied 36 preHD and 22 age‐matched controls using a novel method to quantify regional change from T1‐weighted structural images acquired 1 year apart. We assessed cross‐sectional volume differences and longitudinal volumetric change in 7 subcortical structures—the accumbens, amygdala, caudate, hippocampus, pallidum, putamen, and thalamus. At baseline, accumbens, caudate, pallidum, and putamen volumes were reduced in preHD versus controls (all P < .01). Longitudinally, atrophy was greater in preHD than controls in the caudate, pallidum, and putamen (all P < .01). Each structure showed a large between‐group effect size, especially the pallidum where Cohen's d was 1.21. Using pallidal atrophy as a biomarker, we estimate that a hypothetical 1‐year neuroprotection study would require only 35 preHD per arm to detect a 50% slowing in atrophy and only 138 preHD per arm to detect a 25% slowing in atrophy. The effect sizes calculated for preHD basal ganglia atrophy over 1 year are some of the largest reported to date. Consequently, this translates to strikingly small sample size estimates that will greatly facilitate any future neuroprotection study. This underscores the utility of this automatic image segmentation and longitudinal nonlinear registration method for upcoming studies of preHD and other neurodegenerative disorders. © 2011 Movement Disorder Society
Validation of the MDS‐UPDRS Part I for nonmotor symptoms in Parkinson's disease
Authors: Gallagher David A. , Goetz Christopher G. , Stebbins Glenn , Lees Andrew J. , Schrag Anette 
Published: September 2011

AbstractThe UPDRS has been the main outcome measure in studies of PD. Modifications have been made to improve scale properties and represent the breadth of manifestations of PD, particularly nonmotor symptoms (NMS), resulting in the Movement Disorder Society's revision of the UPDRS (MDS‐UPDRS). This study was undertaken to determine the validity of MDS‐UPDRS Part I (nonmotor experiences of daily living). The MDS‐UPDRS and a number of validated scales for the NMS in PD were used in 94 patients with PD from Hoehn and Yahr stage I to V. We assessed reliability, floor and ceiling effects, and correlations with validated scales for the nonmotor symptoms of PD. MDS‐UPDRS Part I showed high internal consistency (Cronbach's alpha: 0.85), small floor and ceiling effects (2% floor and 0% ceiling effect), and good concurrent validity (correlation with the original UPDRS Part I: r = 0.81, P < 0.001). The standardized z‐score of the MDS‐UPDRS Part I score demonstrated high convergent validity with the composite z‐score of nonmotor scales (r = 0.89, P < 0.0001), and the two subscores based on the original factor analysis of Part I also had high correlations with the composite z‐scores of corresponding nonmotor scales (depression, anxiety, apathy factor score: r = 0.72, P < 0.0001; other nonmotor features factor score: r = 0.87, P < 0.0001). Our data demonstrate that the MDS‐UPDRS Part I total score has a strong relationship with a composite score of validated scales for the nonmotor aspects of PD. © 2011 Movement Disorder Society
Neuropsychological correlates of theory of mind in patients with early Parkinson's disease
Authors: Santangelo Gabriella , Vitale Carmine , Trojano Luigi , Errico Domenico , Amboni Marianna , Barbarulo Anna Maria , Grossi Dario , Barone Paolo 
Published: September 2011

AbstractThe theory of mind is the ability to attribute mental states to oneself and others and to understand that others have beliefs, desires and intentions different from one's own. The aim of the study was to explore the neuropsychological correlates of theory of mind in patients affected by early Parkinson's disease (PD). Thirty‐three PD patients and 33 age‐, sex‐, and education‐matched control subjects underwent the Frontal Assessment Battery, as well as tasks assessing “cognitive” and “affective” theory of mind, and memory abilities; questionnaires evaluating behavioral disorders and quality of life were also administrated. Although the 2 groups did not differ on neuropsychological tasks, PD patients' performance on tasks assessing cognitive and affective theory of mind was significantly worse than controls. Moreover, PD patients had more behavioral disorders and worse quality of life than controls. After covarying for behavioral and quality of life scores, the differences between patients and controls on theory of mind tasks remained significant. “Cognitive” theory of mind was associated with Frontal Assessment Battery score and 2 domains of quality of life scale, whereas “affective” theory of mind scores correlated only with behavioral scales such as the Frontal Behavioral Inventory and Apathy Evaluation Scale. The results demonstrate that both affective and cognitive aspects of theory of mind are simultaneously impaired in early PD and suggest that deficits in the 2 subcomponents of theory of mind may be linked to dysfunction of different frontosubcortical circuitries in early PD. © 2011 Movement Disorder Society
Effects of protonation and co‐anion on the 1H and 13C chemical shifts of 2r,6c‐ diphenylpiperidin‐4‐ones: evidence for the formation of ion pair
Authors: Vimalraj V. , Pandiarajan K. 
Published: September 2011

Abstract1H and 13C NMR spectra have been recorded for 2r,6c‐diphenylpiperidin‐4‐one (1a), 3t‐alkyl‐2r,6c‐diphenylpiperidin‐4‐ones 1b‐d, 3t‐alkyl‐2r,6c‐diphenyl‐4‐oxopiperidinium nitrates 2b and 2d, 3t‐alkyl‐2r,6c‐diphenylpiperidin‐4‐one hydrochlorides 3a‐c and 3t‐methyl‐2r,6c‐diphenyl‐4‐oxopiperidinium picrate 4b in DMSO‐d6. For 1b, 2b and 3b, 1H and 13C NMR spectra have been recorded in CH3OD also. For 1b, 1d, 2b, 2d, 3b and 4b, 2D spectra have also been recorded. In DMSO‐d6 the protons of the piperidine ring and the ortho protons of the phenyl groups are markedly deshielded due to protonation. Protonation shields all the carbons of the piperidine ring and the ipso carbons of the phenyl groups markedly but deshields the other aromatic carbons slightly. The deshieldings on H‐3a, H‐5a and the ortho protons of the phenyl groups are less in the nitrate and picrate than in the corresponding hydrochloride. The effects on 13C chemical shifts are not influenced by the co‐anion. These observations suggest that the nitrates and pictrate exist as ion pairs in DMSO‐d6 and the nitrate and picrate ions shield, H‐3a, H‐5a and the ortho protons by magnetic anistropic effect. In CH3OD for 2b and 3b in addition to the ion‐pair containing free ions two ion‐pairs containing solvated ions are also present. The effects of protonation in the ion pairs containing the solvated ions are significantly different from those in the ion‐pair containing free ions. Copyright © 2011 John Wiley & Sons, Ltd.
Maximum delay computation for interdomain path selection
Authors: Amigo Isabel , Vaton Sandrine , Chonavel Thierry , Larroca Federico 
Published: September 2011

SUMMARYOne important problem when deploying interdomain path selection is advertising metrics that hold for a long period of time. In this paper we propose a method to aid interdomain path selection mechanisms in that sense. We present a means of computing a bound on the end‐to‐end delay of traversing a domain considering that the traffic varies within a given uncertainty set. This provides a robust and a verifiable quality of service value for traversing the autonomous system (AS), without revealing confidential information. Consequently, the bound can be safely conceived as a metric to be announced by each AS in the process of interdomain path selection. We show how the maximum delay value is obtained for an interdomain bandwidth demand and we propose an exact method and a numerical approximation method for computing it, neither of which rely on a complex monitoring infrastructure. Simulations with real data that illustrate the problem and validate our results are also presented. Copyright © 2011 John Wiley & Sons, Ltd.
The correlation between lung volume and liver herniation measurements by fetal MRI in isolated congenital diaphragmatic hernia: a systematic review and meta‐analysis of observational studies
Authors: Mayer Steffi , Klaritsch Philipp , Petersen Scott , Done Elisa , Sandaite Inga , Till Holger , Claus Filip , Deprest Jan A. 
Published: September 2011

ObjectiveWe conducted a meta‐analysis to assess the correlation of lung volume and liver position measured by magnetic resonance imaging (MRI) with survival until discharge in fetuses with isolated congenital diaphragmatic hernia (CDH).MethodSystematic searches of MEDLINE and EMBASE from 1 January 1980 to 10 December 2010 were performed. Studies correlating total fetal lung volumes (TFLV, observed/expected (O/E) TFLV) and/or liver position by fetal MRI to survival in expectantly managed fetuses with CDH were included. Data on the side of the defect, position of the liver, TFLV, O/E TFLV, gestational age (GA) at MRI, GA and weight at birth were collected. Odds ratio (OR) for dichotomous data, mean differences (MD) or standardized mean differences (SMD) for continuous variables were determined using RevMan 5.0 software.ResultsNineteen studies (n = 602 fetuses) were included. Survival was associated with left‐sided defects (OR 2.52; p = 0.01), “liver down” (OR 0.18; p < 0.00001), a higher TFLV (MD 9.63; p < 0.00001) and O/E TFLV (SMD 0.98; p < 0.00001) as well as higher birth weight (MD 146.60; p = 0.04). GA at MRI (MD 0.70) and GA at birth (MD 0.33) were not correlated with survival.ConclusionsMRI measurements of fetal lung volumes, liver position and side of the defect correlate well with neonatal survival in fetuses with isolated CDH. Copyright © 2011 John Wiley & Sons, Ltd.
Preparation and properties of polyimide solvent‐resistant nanofiltration membrane obtained by a two‐step method
Authors: Fang Bing , Pan Kai , Meng Qinghan , Cao Bing 
Published: September 2011

AbstractThis study investigated the preparation of polyimide solvent‐resistant nanofiltration membranes by a two‐step method (casting the membrane first and then crosslinking by the thermal imidization method). The influences of polymer concentration, thickness of membranes, temperature of the imidization, phase inversion time and thermal imidization procedure were studied. The membranes with the highest rejection rate of Fast Green FCF (molecular weight 808.86 g mol−1) were prepared in the following conditions: polymer concentration 13 wt%, phase inversion time 1 h, membrane thickness 150 µm and thermal imidization procedure 200 °C for 2.5 h, 250 °C for 2 h and 300 °C for 2 h in a vacuum environment; the heating rate was 5 °C min−1. The membrane was stable in most of the solvents tested and the fluxes of some common solvents were equal to or higher than a number of commercial solvent‐resistant nanofiltration membranes. A much higher rejection of dyes in water than in methanol was observed in the filtration experiments and a new way to explain it was developed. Copyright © 2011 Society of Chemical Industry
Effect of aminobisphosphonated copolymer on the thermal stability and flammability of poly(methyl methacrylate)
Authors: Vahabi Hossein , Longuet Claire , Ferry Laurent , David Ghislain , Robin Jean‐Jacques , Lopez‐Cuesta José‐Marie 
Published: September 2011

AbstractMethyl methacrylate was copolymerized with propyl N,N‐tetramethylbis(phosphonate)‐bis(methylene)aminemethyl methacrylate (MAC3NP2). The thermal degradation and flammability of this modified poly(methyl methacrylate) (PMMA) were compared with those of pure PMMA using thermogravimetric analysis (TGA) and pyrolysis combustion flow calorimetry (PCFC). The morphology of char was investigated using scanning electron microscopy and the yield of phosphorus using energy‐dispersive X‐ray analysis. The gases evolved during degradation in TGA were analysed using Fourier transform infrared spectroscopy. The total heat release and heat release capacity of the reactively modified PMMA are reduced, as compared to pure PMMA. The modified PMMA presents a better thermal stability (above 290 °C) than pure PMMA and leads to an important char formation. A comparison among TGA, PCFC and the amount of phosphorus in the condensed phase gives useful information about the role of phosphorus in the flame retardancy of the copolymer. The result reveals the effect of phosphorus not only in the condensed phase but also in the vapour phase. Copyright © 2011 Society of Chemical Industry
Retracted: Cypermethrin exposure leads to regulation of proteins expression involved in neoplastic transformation in mouse skin
Authors: George Jasmine , Srivastava Amit Kumar , Singh Richa , Shukla Yogeshwer 
Published: September 2011

AbstractCypermethrin, a synthetic pyrethroid insecticide is shown to exert carcinogenic effects in rodents; however, its underlying mechanism remains elusive. Here, we showed the effect of cypermethrin on protein expression involved in neoplastic transformation in mouse skin. Comparative protein expression profiles between untreated control and cypermethrin‐treated mouse skin were explored using 2‐DE. A total of 27 spots that were statistically significant (p<0.05) and differentially expressed in response to cypermethrin exposure were identified by MALDI‐TOF/TOF and LC‐MS/MS. Among them, six up‐regulated proteins (carbonic anhydrase 3 (Ca 3), Hsp‐27, S100A6, galectin‐7, S100A9, S100A11) and one down‐regulated protein (superoxide dismutase [Cu‐Zn] (Sod 1)) are associated with cancer‐related key processes. These selected dysregulated proteins were further validated in 2‐DE gels of mouse skin treated with known tumorigens (benzo‐[a]‐pyrene, 12‐O‐tetradecanoyl‐phorbol‐13‐acetate and mezerein), respectively. Comparative studies showed that Ca 3, S100A6, S100A9, S100A11 and Sod 1 are specific for stages of development and progression of tumors whereas Hsp‐27 and galectin‐7 are specific for tumor promotion stage by cypermethrin in mouse skin. Furthermore, these chosen proteins confirmed by Western blotting and immunofluorescence staining were consistent with changes in 2‐DE check. This proteomic investigation for the first time provides key proteins that will contribute in understanding the mechanism behind cypermethrin‐induced neoplastic transformation.