bluuebunny/crossref_metadata_2025_split huggingface dataset:
Involvement of threonine 258 and serine 259 motif in amphetamine‐induced norepinephrine transporter endocytosis
Authors: Annamalai Balasubramaniam , Mannangatti Padmanabhan , Arapulisamy Obulakshmi , Ramamoorthy Sammanda , Jayanthi Lankupalle D. 
Published: July 2010

J. Neurochem.(2010)115, 23–35.Abstractd‐Amphetamine (AMPH) down‐regulates the norepinephrine transporter (NET), although the exact trafficking pathways altered and motifs involved are not known. Therefore, we examined the cellular and molecular mechanisms involved in AMPH‐induced NET regulation in human placental trophoblast cells expressing the wild‐type (WT)‐hNET and the hNET double mutant (DM)‐bearing protein kinase C (PKC)‐resistant T258A + S259A motif. NET function and surface expression were significantly reduced in cells expressing WT‐hNET but not in cells expressing hNET‐DM following AMPH treatment. AMPH inhibited plasma membrane recycling of both WT‐hNET and hNET‐DM. In contrast, AMPH stimulated endocytosis of WT‐hNET, and did not affect hNET‐DM endocytosis. Although PKC or calcium/calmodulin‐ dependent kinase‐II (CaMKII) inhibition or depletion of calcium failed to block AMPH‐mediated down‐regulation of WT‐hNET, NET‐specific blocker desipramine completely prevented AMPH‐induced down‐regulation. Furthermore, AMPH treatment had no effect on phospho‐CaMKII immunoreactivity. The inhibitory potency of AMPH was highest on hNET‐DM, intermediary on T258A and S259A single mutants and lowest on WT‐hNET. Single mutants exhibited partial resistance to AMPH‐mediated down‐regulation. AMPH accumulation was similar in cells expressing WT‐hNET or hNET‐DM. The results demonstrate that reduced plasma membrane insertion and enhanced endocytosis account for AMPH‐mediated NET down‐regulation, and provide the first evidence that T258/S259 motif is involved only in AMPH‐induced NET endocytosis that is desipramine‐sensitive, but PKC and CaMKII independent.
Can the patient perspective contribute to quality of nutritional care?
Authors: Holst Mette , Rasmussen Henrik H , Laursen Birgitte S 
Published: July 2010

Scand J Caring Sci; 2011; 25; 176–184 Can the patient perspective contribute to quality of nutritional care?Aim: Undernutrition has been seen in hospitalized patients at all times. Nurses have a central position in the nutritional care of the patient. Despite guidelines for nutritional practise and care, 20–55% of patients are still at risk of complications to insufficient nutrition intake. The aim of this study was to obtain knowledge of hospitalized patient’s experiences of being undernourished, to understand implications this might have to quality of nutritional nursing care.Methods: Qualitative interviews were undertaken in 12 hospitalized patients at severe nutritional risk.Findings: Pain, no appetite, bad taste and side effects to medication were among reasons for poor eating. Nurses practically did not address or question symptoms that could influence poor eating and were only to a low degree involved at patient initiative. The patients appeared to divide into two groups; One ‘Passive group’, characterized with fatigue, lack of concentration and short term memory, were found insusceptible to increase nutrition intake by motivation and guidance. The overall motivation in the other ‘Active’ group was the setting of and achievement of goals, which had to be clear, communicated and followed up by nurses and physicians. This group furthermore found self‐determination and active involvement determinant for a positive and fruitful cooperation between staff and themselves.Conclusions: Nurses were in progressive about clarifying why patients did not eat. Patients highly regarded nutrition registration when followed up, however, this was only practised to a low degree. Severely undernourished patients could be divided in two groups. The ‘Passive group’ should be attended to systematically. Artificial nutrition is often needed in this group. The ‘Active group’ should be cared for with individual, active involvement from nurses. Nurses should be able to distinct which nutritional care for which patient, and act upon it.
BIODIVERSITY RESEARCH: Genetic diversity in two introduced biofouling amphipods (Ampithoe valida & Jassa marmorata) along the Pacific North American coast: investigation into molecular identification and cryptic diversity
Authors: Pilgrim Erik M. , Darling John A. 
Published: July 2010

AbstractAim  We investigated patterns of genetic diversity among invasive populations of Ampithoe valida and Jassa marmorata from the Pacific North American coast to assess the accuracy of morphological identification and determine whether or not cryptic diversity and multiple introductions contribute to the contemporary distribution of these species in the region.Location  Native range: Atlantic North American coast; Invaded range: Pacific North American coast.Methods  We assessed indices of genetic diversity based on DNA sequence data from the mitochondrial cytochrome c oxidase subunit I (COI) gene, determined the distribution of COI haplotypes among populations in both the invasive and putative native ranges of A. valida and J. marmorata and reconstructed phylogenetic relationships among COI haplotypes using both maximum parsimony and Bayesian approaches.Results  Phylogenetic inference indicates that inaccurate species‐level identifications by morphological criteria are common among Jassa specimens. In addition, our data reveal the presence of three well supported but previously unrecognized clades of A. valida among specimens in the north‐eastern Pacific. Different species of Jassa and different genetic lineages of Ampithoe exhibit striking disparity in geographic distribution across the region as well as substantial differences in genetic diversity indices.Main conclusions  Molecular genetic methods greatly improve the accuracy and resolution of identifications for invasive benthic marine amphipods at the species level and below. Our data suggest that multiple cryptic introductions of Ampithoe have occurred in the north‐eastern Pacific and highlight uncertainty regarding the origin and invasion histories of both Jassa and Ampithoe species. Additional morphological and genetic analyses are necessary to clarify the taxonomy and native biogeography of both amphipod genera.
Torsade de pointes induced by ioxaglate intracoronary injection in patients with pre‐existent drug‐induced QT prolongation: case reports and review of literature
Authors: Andréjak Michel , Leborgne Laurent , Tribouilloy Christophe , Andréjak Marie‐thérèse , Caron Jacques 
Published: July 2010

AbstractWe report two cases of torsade de pointes directly related to intracoronary contrast media injection in patients without previous history of neither arrhythmia nor syncope but chronically treated with a drug prolonging ventricular repolarization. We discussed the effects of the contrast medium used on repolarization and concluded that three suggestions may be highlighted from the case reports presented and from the literature: (i) a QT prolongation should be systematically searched before coronary angiography; (ii) it seems important to correct QT prolongation when it results from a reversible cause (such as drug‐induced) before nonurgent coronary angiography; and (iii) if there is no reversible cause explaining QT prolongation, contrast media should be used cautiously in such patient and nonionic iso‐osmolar contrast media should be preferred.
Inferring Hidden Causal Structure
Authors: Kushnir Tamar , Gopnik Alison , Lucas Chris , Schulz Laura 
Published: July 2010

AbstractWe used a new method to assess how people can infer unobserved causal structure from patterns of observed events. Participants were taught to draw causal graphs, and then shown a pattern of associations and interventions on a novel causal system. Given minimal training and no feedback, participants in Experiment 1 used causal graph notation to spontaneously draw structures containing one observed cause, one unobserved common cause, and two unobserved independent causes, depending on the pattern of associations and interventions they saw. We replicated these findings with less‐informative training (Experiments 2 and 3) and a new apparatus (Experiment 3) to show that the pattern of data leads to hidden causal inferences across a range of prior constraints on causal knowledge.
The clinical and radiographic outcome of implants placed in the posterior maxilla with a guided flapless approach and immediately restored with a provisional rehabilitation: a randomized clinical trial
Authors: Van de Velde T. , Sennerby L. , De Bruyn H. 
Published: July 2010

AbstractObjectives: To evaluate and compare the outcome of dental implants placed using a flapless protocol and immediate loading with a conventional protocol and loading after 6 weeks.Materials and methods: Fourteen patients with bilateral maxillary edentulous areas were treated using Straumann SLA‐implants. Using a randomized split‐mouth design, implants were placed in one side of the maxilla using a stereolithographic surgical guide for flapless surgery and immediately loaded on temporary abutments with a bridge (test). Implants in the other side were placed using the conventional protocol and loaded after 6 weeks of healing (control). Clinical and radiographic evaluation of peri‐implant tissues was performed at time of implant surgery, and after 1 week, 6 weeks, 3, 6, 12 and 18 months.Results: A total of 70 implants were placed (36 test and 34 control). One implant (test) was lost after 3 months, resulting in a survival rate of 97.3% for the test implants and 100% for the control implants. Marginal bone levels were not statistically significantly different between the test and control implants but at baseline the marginal bone level was significantly lower compared to the other evaluation periods (P < 0.05). The mean bone level for test and control implants was 1.95 mm ± 0.70 and 1.93 mm ±0.42 after 18 months, respectively. There was a significant change in height of the attached mucosa at implants placed with a conventional flap between post‐operative and 1 week and between 1 week and 6 weeks. Statistically significant differences were found between the test side and the control side for opinion about speech, function, aesthetics, self‐confidence and overall appreciation the first 6 weeks.Conclusion: Implants can successfully integrate in the posterior maxilla using a flapless approach with immediate loading similar to a conventional protocol. The mucosal tissues around implants placed with a conventional flap changed significantly compared with flapless placed implants. To cite this article:
Van de Velde T, Sennerby L, De Bruyn H. The clinical and radiographic outcome of implants placed in the posterior maxilla with a guided flapless approach and immediately restored with a provisional rehabilitation: a randomized clinical trial. Clin. Oral Impl. Res. 21, 2010; 1223–1233. doi: 10.1111/j.1600‐0501.2009.01924.x
Spontaneous early exposure and marginal bone loss around conventionally and early‐placed submerged implants: a double‐blind study
Authors: Çehreli Murat Cavit , Kökat Ali Murat , Uysal Serdar , Akca Kivanc 
Published: July 2010

AbstractPurpose: The aim of this retrospective study was to compare the frequency of spontaneous early exposure of cover screws and marginal bone resorption in conventionally and early‐placed submerged implants before second‐stage surgery.Materials and methods: A total of 103 Nobel Biocare Branemark implants were conventionally (Group 1), or early‐placed (Group 2) in 46 consecutive patients following the two‐stage surgical protocol. Patients in both groups received oral hygiene training in self‐performed plaque control measures, including exposure of cover screws during healing. Spontaneous cover screw exposure (CSE) of each implant was recorded for both groups and scored from Class 0 (no perforation) to Class 4 (complete exposure). Plaque index scores were recorded and marginal bone‐level (MBL) changes were measured in radiographs before second‐stage surgery in a blind manner.Results: MBL in Group 2 was higher than Group 1 in patients with or without interim prosthesis (P<0.05). The use of interim prosthesis did not increase MBL in Group 1, but led to higher MBL in Group 2. The percentage of non‐exposed implants in Group 1 was higher than Group 2 (P=0.007, odds ratio=7). Group 1 implants had 11.5 times greater plaque index score 0 than those in Group 2 (P=0.031, odds ratio=11.5). The differences between MBL with regard to CSE scores 0 and 1–4 was significant for both sides in Group 2 and the mesial side in Group 1 (P<0.05). The difference between MBL with regard to plaque index scores 1–3 was similar in both groups (P>0.05).Conclusions: There is a direct relation between spontaneous early cover screw perforations with early crestal bone loss. Early‐placed implants experienced more spontaneous perforations and associated bone loss in comparison with conventionally placed submerged implants. The use of interim dentures may lead to more CSE and consequent MBL in the early‐placement protocol. To cite this article:
Çehreli MC, Kökat AM, Uysal S, Akca K. Spontaneous early exposure and marginal bone loss around conventionally and early‐placed submerged implants: a double‐blind study. Clin. Oral Impl. Res. 21, 2010; 1327–1333. doi: 10.1111/j.1600‐0501.2009.01952.x
Alumina‐reinforced zirconia implants: survival rate and fracture strength in a masticatory simulation trial
Authors: Kohal Ralf‐J. , Wolkewitz Martin , Mueller Constanze 
Published: July 2010

AbstractBackground: Alumina‐toughened zirconia (ATZ) is a possible alternative material to titanium for oral implants. No data are available on the fracture strength of ATZ oral implants.Purpose: The purpose of this study was to examine one‐piece implants made of ATZ ceramic under artificial loading conditions and to compare the fracture strength of these implants with implants fabricated from tetragonal zirconium dioxide poylcrystal (TZP)‐A.Materials and methods: A total of 72 implants, 48 ATZ implants (groups A and B) and 24 TZP‐A implants (group C), were investigated. A chamfer preparation at the implant heads was performed on all implants of groups B and C. Eight implants of each group underwent 1.2 or five million thermomechanical loading cycles in the chewing simulator (load value: 98 N). Further eight implants of each group were not cyclic loaded. Finally, the fracture strength of all implants was determined using a universal testing machine.Results: No implant fractured during loading in the chewing simulator. All implants were placed in the universal testing machine to evaluate fracture strength. The mean fracture strength values±standard deviations for the implants without artificial loading were 1734±165 N (ATZ, no preparation), 1220±85 N (ATZ, with preparation), and 578±49 N (TZP‐A, with preparation); 1489±190 N (ATZ, no preparation), 1064±121 N (ATZ, with preparation), and 607±57 N (TZP‐A, with preparation) with 1.2 million loading cycles; and 1358±187 N (ATZ, no preparation), 1098±97 N (ATZ, with preparation), and 516±45 N (TZP‐A, with preparation) with five million cycles. The ATZ implants showed significantly higher mean fracture strengths compared with the TZP‐A implants. Modification of the implant head using diamond burs and increased loading time also led to a significant decrease in fracture strength.Conclusions: The ATZ implants showed an increased mechanical stability compared with the TZP‐A. Modification of the implant head resulted in a decrease in fracture strength. However, within the limits of this in vitro investigation it can be concluded that ATZ implants will withstand functional loading over an estimated period of 20 years. To cite this article:
Kohal R‐J, Wolkewitz M, Mueller C. Alumina‐reinforced zirconia implants: survival rate and fracture strength in a masticatory simulation trial. Clin. Oral Impl. Res. 21, 2010; 1345–1352. doi: 10.1111/j.1600‐0501.2010.01954.x
This article has been retracted: A preliminary examination of the role of NFAT 3 in human skin, cultured keratocytes and dermal fibroblasts
Authors: Al‐Daraji Wael I. 
Published: July 2010

Retraction Retraction: The following article from Journal of Cutaneous Pathology, “A preliminary examination of the role of NFAT 3 in human skin, cultured keratocytes and dermal fibroblasts” by Wael I. Al‐Daraji, published online on 8 July, 2010 in Wiley Online Library (http://onlinelibrary.wiley.com), has been retracted by agreement between the journal Editor in Chief, Timothy McCalmont, and Blackwell Publishing Ltd. The retraction has been made as the manuscript was submitted for publication in breach of an agreement with the University of Newcastle upon Tyne due to the failure to provide appropriate acknowledgement of sources. Background: Ciclosporin A (CsA) is widely utilized for the treatment of inflammatory skin diseases such as psoriasis.The therapeutic effects of CsA are thought to be mediated via its immunosuppressive action on infiltrating lymphocytes in skin lesions. CsA and tacrolimus block T cell activation by inhibiting the phosphatase calcineurin and preventing translocation from the cytoplasm to the nucleus of the transcription factor Nuclear Factor of Activated T cells (NFAT). Methods: RT‐PCR and Western Analysis were used to investigate the presence of NFAT‐3 mRNA and protein in human keratocytes. Tissue culture of human keratocytes and immunostaining of cells on coverslips and confocal microscopy were used to assess the degree of nuclear localisation of NFAT‐3 in cultured cells. Keratome biopsies were taken from patients with psoriasis (lesional and non‐lesional skin) and normal skin and immunohistochemistry was used to assess the NFAT‐3 localisation in these biopsies using a well characterized anti‐NFAT‐3 antibody. Results: The NFAT‐3 mRNA and protein expression was demonstrated using RT‐PCR and Western blotting. The expression of NFAT‐3 in human keratocytes and response to different agonists provides perhaps a unique opportunity to examine the regulation, subcellular localization and kinetics of translocation of different NFATs in primary cultured human cells. As with NFAT 1, NFAT 2 and recently NFAT 5, differentiation‐promoting agents that increase intracellular calcium concentration induced nuclear translocation of NFAT‐3 in cultured keratocytes but with different kinetics. Conclusion: These data provide the first evidence of that NFAT‐3 is expressed in normal skin, psoriasis and that NFAT‐3 functionally active in human keratocytes and that nuclear translocation of NFAT‐3 in human skin cells has different kinetics than NFAT 1 suggesting that NFAT‐3 may play an important role in regulation of keratocytes proliferation and differentiation at a different stage. Inhibition of this pathway in human epidermal keratocytes many account, in part for the therapeutic effects of CsA and tacrolimus in skin disorders such as psoriasis.Al‐Daraji WI. A preliminary examination of the role of NFAT 3 in human skin, cultured keratocytes and dermal fibroblasts.
Missing C‐terminal filaggrin expression, NFkappaB activation and hyperproliferation identify the dog as a putative model to study epidermal dysfunction in atopic dermatitis
Authors: Chervet Ludovic , Galichet Arnaud , McLean W. H. Irwin , Chen Huijia , Suter Maja M. , Roosje Petra J. , Müller Eliane J. 
Published: July 2010

Please cite this paper as: Missing C‐terminal filaggrin expression, NFkappaB activation and hyperproliferation identify the dog as a putative model to study epidermal dysfunction in atopic dermatitis. Experimental Dermatology 2010; 19: e343–e346.Abstract:  Filaggrin loss‐of‐function mutations resulting in C‐terminal protein truncations are strong predisposing factors in human atopic dermatitis (AD). To assess the possibility of similar truncations in canine AD, an exclusion strategy was designed on 16 control and 18 AD dogs of various breeds. Comparative immunofluorescence microscopy was performed with an antibody raised against the canine filaggrin C‐terminus and a commercial N‐terminal antibody. Concurrent with human AD‐like features such as generalized NFKB activation and hyperproliferation, four distinctive filaggrin expression patterns were indentified in non‐lesional skin. It was found that 10/18 AD dogs exhibited an identical pattern for both antibodies with comparable (category I, 3/18) or reduced (category II, 7/18) expression to that of controls. In contrast, 4/18 dogs displayed aberrant large vesicles revealed by the C‐terminal but not the N‐terminal antibody (category III), while 4/18 showed a control‐like N‐terminal expression but lacked the C‐terminal protein (category IV). The missing C‐terminal filaggrin in category IV strongly points towards loss‐of function mutations in 4/18 (22%) of all AD dogs analysed.
The curious case of J113924.74+164144.0: a possible new group of galaxies at z = 0.069
Authors: Roy Nirupam , Sengupta Chandreyee , Kantharia N. G. 
Published: July 2010

Abstract
J113924.74+164144.0 is an interesting galaxy at z = 0.0693, i.e. DL∼ 305 Mpc, with tidal-tail-like extended optical features on both sides. There are two neighbouring galaxies, a spiral galaxy J113922.85+164136.3 which has a strikingly similar ‘tidal’ morphology and a faint galaxy J113923.58+164129.9. We report H i 21-cm observations of this field to search for signatures of possible interaction. Narrow H i emission is detected from J113924.74+164144.0, but J113922.85+164136.3 shows no detectable emission. The total H i mass detected in J113924.74+164144.0 is 7.7 × 109 M⊙. The H i emission from the galaxy is found to be extended and significantly offset from the optical position of the galaxy. We interpret this as signature of possible interaction with the neighbouring spiral galaxy. There is also a possible detection of H i emission from another nearby galaxy J113952.31+164531.8 at z = 0.0680 at a projected distance of 600 kpc, and with a total H i mass of 5.3 × 109 M⊙, suggesting that all these galaxies form a loose group at z ∼ 0.069.
Protective effect of single‐dose adjuvanted pandemic influenza vaccine in children
Authors: Van Buynder P. G. , Dhaliwal J. K. , Van Buynder J. L. , Couturier C. , Minville‐LeBlanc M. , Garceau R. , Tremblay F.‐W. 
Published: July 2010

Please cite this paper as: Van Buynder et al. (2010) Protective effect of single‐dose adjuvanted pandemic influenza vaccine in children. Influenza and Other Respiratory Viruses 4(4), 171–178.Background  During the first wave of A/California/7/2009(H1N1) influenza, high rates of hospitalization in children under 5 years were seen in many countries. Subsequent policies for vaccinating children varied in both type of vaccine and number of doses. In Canada, children 36 months to <10 years received a single dose of 0·25 ml of the GSK adjuvanted vaccine (Arepanrix™) equivalent to 1·9 μg HA. Children 6 months to 35 months received two doses as did those 36–119 months with chronic medical conditions.Method  We conducted a community‐based case–control vaccine effectiveness (VE) review of children under 10 years with influenza like illness who were tested for H1N1 infection at the central provincial laboratory. Laboratory‐confirmed influenza was the primary outcome, and vaccination status the primary exposure to assess VE after a single 0·25‐ml dose.Results  If vaccination was designated to be effective after 14 days, no vaccinated child had laboratory‐confirmed influenza compared to 38% of controls. The VE of 100% was statistically significant for children <10 years of age and <5 years considered separately. If vaccination was considered effective after 10 days, VE dropped to 96% overall but was statistically significant and over 90% in all age subgroups, including those under 36 months.Conclusions  A single 0·25‐ml dose of the GSK adjuvanted vaccine (Arepanrix™) protects children against laboratory‐confirmed pandemic influenza potentially avoiding any increased reactogenicity associated with second doses. Adjuvanted vaccines offer hope for improved seasonal vaccines in the future.