bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of genes involved in scent and color production in Rosa Damascena Mill
Authors: Kiani Hoda Sadat , Noudehi Manijeh Sabokdast , Shokrpour Majid , Zargar Meisam , Naghavi Mohammad Reza 
Published: June 2024

Abstract

Rosa damascena Mill., commonly known as the King Flower, is a fragrant and important species of the Rosaceae family. It is widely used in the perfumery and pharmaceutical industries. The scent and color of the flowers are significant characteristics of this ornamental plant. This study aimed to investigate the relative expression of MYB1, CCD1, FLS, PAL, CER1, GT1, ANS and PAR genes under two growth stages (S1 and S2) in two accessions. The CCD1 gene pathway is highly correlated with the biosynthesis of volatile compounds. The results showed that the overexpression of MYB1, one of the important transcription factors in the production of fragrance and color, in the pink accession of sample S2 increased the expression of PAR, PAL, FLS, RhGT1, CCD1, ANS, CER1, and GGPPS. The methyl jasmonate stimulant had a positive and cumulative effect on gene expression in most genes, such as FLS in ACC.26 of the S2 sample, RhGT1, MYB1, CCD1, PAR, ANS, CER1, and PALin ACC.1. To further study, a comprehensive analysis was performed to evaluate the relationship between the principal volatile compounds and colors. Our data suggest that the rose with pink flowers had a higher accumulation content of flavonoids and anthocyanin. To separate essential oil compounds, GC/MS analysis identified 26 compounds in four samples. The highest amount of geraniol, one of the main components of damask rose, was found in the dark-pink flower, 23.54%, under the influence of the methyl jasmonate hormone.
Development of an Ultrasound-Based Clinical Decision Rule to Rule-out Diverticulitis
Authors: Selame Lauren Ann , Shokoohi Hamid , Loesche Michael 
Published: June 2024

Abstract

The concern for diverticulitis often leads to the use of computed tomography (CT) scans for diagnosis. We aim to develop an ultrasound-based clinical decision rule (CDR) to confidently rule-out the disease without requiring a CT scan. We analyzed data from a prospective study of adult emergency department (ED) patients with suspected diverticulitis who underwent both bedside ultrasound (US) and CT. Patient history, physical examination, laboratory findings, and US results were used to create a CDR via a recursive partitioning model designed to prioritize sensitivity, with a loss matrix that heavily penalized false negatives. We calculated test characteristics for the CDR (TICS-Rule) and assessed the potential reduction in CT scans and ED length of stay. Data from 149 patients (84 female; mean age 58 ± 16) were used to develop the TICS-Rule. The final model integrates US diagnosis of simple and complicated diverticulitis with variables of heart rate, age, history of diverticulosis, vomiting, and leukocytosis. Negative US findings and a heart rate below 100 effectively excluded diverticulitis. The sensitivity increased from 54.5% (32.2–75.6) in the US alone to 100% (84.6–100%) for complicated diverticulitis in the model. The TICS-Rule missed no cases of complicated diverticulitis but one case of simple diverticulitis. The median time from ED greeting to US interpretation was 103 minutes (IQR 62–169), compared to 285 minutes (IQR 229–372) for CT. The TICS-Rule, developed using patient history, labs, and US findings, effectively excludes diverticulitis without the need for CT scans. With high sensitivity and reduced time compared to CT, it offers a promising approach to enhancing clinical decision-making, leading to CT and ED length of stay reductions.
Associations among authoritative leadership, organizational climate, job embeddedness and voice behavior in clinical nurses: a cross-sectional study in China
Authors: Luo Yixin , Chen Jianing , Zhang Yiqiu , Lu Guangli , Chen Chaoran 
Published: June 2024

Abstract

Introduction: The voice behavior of clinical nurses significantly influences the development of the healthcare industry. Understanding the factors influencing clinical nurses' voice behavior can help enhance the effectiveness of leadership styles across different cultures, promote a positive organizational climate, and increase employees' sense of job embeddedness, thereby encouraging more voice behavior. Therefore, this study examines the relationships between authoritative leadership, organizational climate, job embeddedness, and voice behavior.
Aim: The aim of this study was to examine the chain mediating effects of organizational climate and job embeddedness on the relationship between authoritativeleadership and clinical nurses’ voice behavior in China.
Methods: A cross-sectional survey study. We employed a convenient sampling method to recruit 809 clinical nurses from 5 comprehensive hospitals in Henan Province, China from October to November 2023.The scale questionnaires were used to assess, SPSS 25.0 was used for data analysis, and AMOS 26.0 software was used to examine the relationship between variables.
Results: The results showed that authoritative leadership and voice behavior were significantly negatively correlated (r = - 0.18, P＜0.01), and organizational climateand job embeddedness were positively correlated with voice behavior (r = 0.27, P＜0.01; r = 0.40, P＜0.01). Organizational climate and job embeddedness played a chain mediating role in the relationship between authoritative leadership and voice behavior of clinical nurses, accounting for 31.41% of the total effect.
Conclusions: Authoritative leadership has a negative impact on the clinical nurses’ voice behavior. More voice behavior may be taken by choosing appropriate leadership styles, such as recognizing potential drawbacks of authoritative leadership. Meanwhile, organizational climate and job embeddedness acted as a chain mediator between authoritative leadership and clinical nurses’ voice behavior. Nursing managers should focus on developing a positive organizational climate, enhancing employees' sense of job embeddedness, and promoting positive voice behavior among clinical nurses.
Blue Carbon in a Sub-Antarctic Marine Protected Area: Current and Future Perspectives
Authors: Bergagna Lucía , Lovrich Gustavo , Riccialdelli Luciana , Sahade Ricardo 
Published: June 2024

Abstract

Carbon fixation, storage, and eventual sequestration by marine ecosystems are known as “blue carbon”. This carbon uptake by the oceanic biological pump reduces atmospheric carbon dioxide (CO2) and is a major negative feedback mechanism to climate change. Benthic assemblages and their related Nature Contributions to People in Namuncurá – Burdwood Bank I and II (BB), two offshore sub-Antarctic Marine Protected Areas (MPAs), are the conservation values of these MPAs. Here, we show that the C reservoirs of these MPAs can be greater than those of their Antarctic counterparts, which, together with their extension, highlights their relevance. Organic and inorganic carbon were measured in the sediments and macrozoobenthic assemblages. More carbon was stored in the sediments than in the macrobenthic organisms, and the inorganic fraction largely exceeded the organic fraction. Most carbon assessments have focused only on the organic fraction, probably due to the complexity of processes involved in CaCO3 deposition, which starts releasing CO2. We compare various approaches for incorporating carbonates into carbon estimation and underscore the need to consider them because of their high abundance. Additionally, this work highlights the importance of sub-Antarctic benthic ecosystems as nature-based solutions to climate change.
Home-based urinary HPV self-sampling for the detection of cervical cancer precursor lesions: attitudes and preferences from Belgian females participating in the CASUS study
Authors: Hendrickx Jhana O. , Keer Severien Van , Donders Gilbert , Weyers Steven , Doyen Jean , Beyers Koen C.L. , Rios-Cortes Alejandra , Meers Nette , Téblick Laura , Vankerckhoven Vanessa V.J. 
Published: June 2024

Abstract

Background
Cervical cancer (CC) is the fourth most common cancer globally in females, caused by oncogenic infections with high-risk human papillomavirus (hrHPV) strains. Successful CC screening programs strongly depend on the participation rate of the target populationNevertheless, it remains challenging to reach under screened populations, including those with an increased CC risk. The CASUS study aimed to develop a complete CC screening solution based on first-void urine (FVU) self-sampling. Here we report on the usability perceptions and preferences from females that participated in the CASUS study by collecting FVU, also referred to as first-catch urine, as a liquid biopsy.
Methods
Females self-collected FVU samples at home the day before colposcopy using the Colli-Pee® UCM FV-5010, a FVU collection device prefilled with 3 mL of UCM preservative, collecting a total volume of 10mL. Afterwards, they completed a questionnaire expressing their usability perceptions and preferences regarding the device.
Results
A total of 332 females (26-70y) were enrolled in the CASUS study of which 210 completed the questionnaire. Overall, 66.6% of females preferred FVU self-sampling over a physician taken cervical sample (PTS) (32.9%) for their next CC screening. Out of 159 women who reported prior experience with a urine cup, 79.2% () expressed a preference for using the Colli-Pee® UCM FV-5010, while 20.8% favored the traditional urine cup. Additionally, 96.6% () of females found Colli-Pee® UCM FV-5010 easy to use and 97.1% would use the device again. A total of 208 valid System Usability Score (SUS) scores were received with an average of 86.17 ± 1.03 Standard Error of Mean (SEM).
Conclusion
The results of this study show that the majority of females in this referral cohort would prefer to self-collect a FVU sample at-home over a PTS for their next CC screening. Moreover, Colli-Pee® UCM FV-5010 was considered an easy-to-use and well-accepted self-sampling device for CC screening in a Belgian colposcopy referral population. From a future perspective, these results highlight the possibility of home-based FVU self-sampling as a liquid biopsy in CC screening where under screened populations could be approached more easily.
Trial registration:
The CASUS study was registered in ClinicalTrials.gov (identifier: NCT04530201).
Spike-in enhanced phosphoproteomics uncovers synergistic signaling responses to MEK inhibition in colon cancer cells
Authors: Selbach Matthias , Bentum Mirjam van , Klinger Bertram , Sieber Anja , Lehmann Nadine , Haji Mohamed , Naghiloo Sheyda , Niquet Sylvia , Mertins Philipp , Blüthgen Nils 
Published: June 2024

Abstract

Targeted kinase inhibitors are a cornerstone of cancer therapy, but their success is often hindered by the complexity of cellular signaling networks that can lead to resistance. Overcoming this challenge necessitates a deep understanding of cellular signaling responses. While standard global phosphoproteomics offers extensive insights, lengthy processing times, the complexity of data interpretation, and frequent omission of crucial phosphorylation sites limit its utility. Here, we combine data-independent acquisition (DIA) with spike-in of synthetic heavy stable isotope-labeled phosphopeptides to facilitate the targeted detection of particularly informative phosphorylation sites. Our spike-in enhanced detection in DIA (SPIED-DIA) approach integrates the improved sensitivity of spike-in-based targeted detection with the discovery potential of global phosphoproteomics into a simple workflow. We employed this method to investigate synergistic signaling responses in colorectal cancer cell lines following MEK inhibition. Our findings highlight that combining MEK inhibition with growth factor stimulation synergistically activates JNK signaling in HCT116 cells. This synergy emphasizes the therapeutic potential of concurrently targeting MEK and JNK pathways, as evidenced by the significantly impaired growth of HCT116 cells when treated with both inhibitors. Our results demonstrate that SPIED-DIA effectively identifies synergistic signaling responses in colorectal cancer cells, presenting a valuable tool for uncovering new therapeutic targets and strategies in cancer treatment.
Brazilian version of the “Primary Sjögren’s Syndrome - Quality of Life questionnaire (PSS-QoL)”: translation, cross-cultural adaptation and validation
Authors: Miyamoto Samira Tatiyama , Serrano Érica Vieira , Gianórdoli Ana Paula Espíndula , Altoé Lara Betini , Noronha Bianca Domingos , Santos Pedro Henrique Alves dos , Pedrini Ana Paula Truhlar , Silva Nicole Reis Souza da , Favarato Letícia Fonseca , Alochio Luíza Vallory , Tomé Weider Andrade , Lackner Angelika , Valim Valéria 
Published: June 2024

Abstract

Background
People with disabilities frequently experience barriers in seeking healthcare that lead to poorer health outcomes compared to people without disabilities. To overcome this, it is important to assess the accessibility of primary health facilities – broadly defined to include a disability-inclusive service provision – so as to document present status and identify areas for improvement. We aimed to identify, adapt and pilot test an appropriate tool to assess the accessibility of primary health facilities in Luuka District, Uganda.
Methods
We conducted a rapid literature review to identify appropriate tools, selecting the Disability Awareness Checklist (DAC) on account of its relative brevity and development as a sensitization and action tool. We undertook three rounds of adaptation, working together with youth researchers (aged 18 – 35) with disabilities who then underwent 2 days of training as DAC facilitators. The adapted tool comprised 71 indicators across four domains and 12 sub-domains. We also developed a structured feedback form for facilitators to complete with healthcare workers. We calculated mean accessibility scores overall, per domain and per sub-domain, and categorised feedback form suggestions by type and presumed investment level. We pilot-tested the adapted tool in 5 primary health facilities in one sub-district of Luuka, nested within a pilot healthcare worker training on disability.
Results
The average overall facility accessibility score was 19.7% (range 12.3 – 28.8). Facility scores were highest in the universal design and accessibility domain (29.7%, 22.6 – 41.9), followed by reasonable accommodation (21.3%, 6.7 – 33.3). Average scores for capacity of facility staff (9.3%, 6.7 – 20.0), and linkages to other services were lower (5.0%, 0 – 25.0). Within the feedback forms, there were on average 21 suggestions (range 14 – 26) per facility. Most commonly, these were categorised as minor structural changes (20% of suggestions), with a third categorised as no (2%) or low (33%) cost, and the majority (40%) medium cost.
Conclusions
Overall accessibility scores were low, with many opportunities for low-cost improvement at the facility level. We did not identify any issues with the implementation of the tool, suggesting few further adaptations are required for its future use in this setting.
The relationship between experienced benefits and harms of using complementary and integrative health approaches and intention to maintain their use in Finland
Authors: Pyykkönen Maija , Ahola Salla , Aarva Pauliina , Pasanen Matti , Helin Kaija 
Published: June 2024

Abstract

Background
The estimated prevalence of the use of complementary and integrative health (CIH) approaches varies between 24–71% in adult populations worldwide. Little is known about the intention to maintain their use. This study aimed to examine whether the experienced benefits and harms of CIH use, and self-rated health (SRH) relate to the intention to use CIH in the future in current users.
Methods
A total of 1652 Finnish CIH users completed an online-survey in December 2022, out of which 58.8% intended to use CIH in the future. Binary logistic regression analyses were used to examine the relationship between the intention to use CIH, experienced benefits and harms of CIH use and SRH.
Results
Having experienced at least one type of benefit from CIH use compared to no experienced benefits was related to the intention to use CIH (OR = 19.3, 95%CI = 11.05 to 33.80). Conversely, having experienced at least one harm from CIH use compared to no harms was inversely related to the intention to use CIH (OR = 0.27, 95%CI = 0.20 to 0.35). Easing of pain or related ailment was found to be the most strongly related benefit to the intention to use CIH. SRH was not found to be statistically significantly related to the intention to use CIH.
Conclusions
This study highlights the importance of the benefits and harms CIH users experience on the continuation of CIH use. The intention to maintain use relates to everyday effectiveness of CIH experienced by users.
Machine Learning-Based Mortality Prediction of 90-Day Discharge in Acute Coronary Syndrome Patients
Authors: Zhang Xinyi , Zhao Zhongxing , Guo Xiaoyan , Lin Jiandong , Lin Mingrui , Deng Feng 
Published: June 2024

Abstract

Background
This study aims to develop and validate a novel mortality prediction model to forecast the 90-day mortality risk for patients with ACS (Acute Coronary Syndrome) after discharge.
Methods
We selected 1359 patients from the Medical Information Mart for Intensive Care IV (MIMIC-IV) database as our study cohort and collected 32 clinical indicators within the first 24 hours of their admission. By randomly assigning these patients to a training group and a validation group (with a ratio of 0.65:0.35), we used Least Absolute Shrinkage and Selection Operator (LASSO) regression and bidirectional stepwise logistic regression to identify 7 key variables. Based on these variables, we constructed a mortality prediction model. To evaluate the model's accuracy and reliability, we plotted the Receiver Operating Characteristic (ROC) curve, calculated the Area Under the Curve (AUC), sensitivity, and specificity, and performed calibration analysis, including plotting calibration curves, calculating Brier scores, and conducting Hosmer-Lemeshow goodness-of-fit tests. Additionally, through Decision Curve Analysis (DCA) and comparison with current clinical scoring systems, we further assessed the clinical utility of our model.
Results
Age, SOFA (Sepsis-related Organ Failure Assessment), APS III (Acute Physiology Score III), AG(Anion Gap), RR(Respiratory rate), INR(International normalized ratio), and BUN(Bun urea nitrogen) were identified as independent predictors of 90-day mortality risk. The model demonstrated good diagnostic performance in both the training and validation groups, with AUC values of 0.842 and 0.855, respectively. The Hosmer-Lemeshow test results indicated a good fit for both datasets, with P-values of 0.1626 and 0.4008. The Brier scores were 0.107 for the training set and 0.103 for the validation set, indicating the model's good predictive performance. Compared to existing scoring systems (SOFA, APSIII), DCA showed that our model could provide a higher net benefit in clinical applications.
Conclusion
We identified seven clinical indicators including age, SOFA, APSIII, AG, RR, INR, and BUN as independent prognostic factors for predicting the 90-day all-cause mortality in patients with ACS after discharge. This model can assist ICU physicians to quickly make preliminary clinical decisions for ACS patients in clinical practice.
A mixed methods study of the challenges and prospects of utilizing telemedicine in the delivery of healthcare to Nigerian children
Authors: Peter Igoche David , Danjuma-Karau Kuyet Jemimah , Omokhuale Ejemeirele M. , Yakubu Joel Cherima 
Published: June 2024

Abstract

Background Despite its immense potential, telemedicine has been underutilized for the paediatric population in Nigeria despite unacceptable mortality indices in newborns and children aged less than 5 years. This study identifies the barriers, prospects and benefits of telemedicine utilization to achieve the second target of the third Sustainable Development Goals. Methods The convergent parallel approach with a mixed methods design was used in this study. Interviewer-administered electronic questionnaires were used to obtain data from 57 and 50 mothers in an urban and a rural healthcare facility, respectively, in Abuja, Nigeria. Audio-recorded semistructured in-depth interviews lasting up to 20 minutes were conducted with key informants, including a paediatrician, an ICT expert and a matron. The qualitative data were analysed via an inductive thematic analysis approach. Results Telemedicine awareness was significantly greater among urban respondents than among rural respondents (p < 0.000). Perceptions of telemedicine as inferior to physical consultation, lack of awareness and cost of service, and resource constraints were identified as barriers. Respondents who were unaware of telemedicine were 0.27 times less willing to pay for the services (p = 0.017). Themes generated include resource constraints, standard operating procedures and possible advantages. Telemedicine was found to be beneficial in terms of patient convenience and physical workspace decongestion. Conclusion Public insights into the applicability of telemedicine to newborn and child care and the availability of resources will enhance its utilization, with attendant benefits.
Synthesis, physicochemical and antimicrobial properties of novel imidazolium based ionic liquids
Authors: Tokoyi Vuyolwethu , Pillay Charlene , Kabane Bakusele , Sabiu Saheed , Deenadayalu Nirmala 
Published: June 2024

Abstract

Most of the research groups are now focusing on deep eutectic solvents due to the lack of biodegradation of ionic liquids (ILs), these liquid salts first emerged as promising and alternative “green” solvents for volatile organic solvents. Ionic liquids have a wide variety of applications with interesting and appealing properties because of various cation and anion combinations that expand their application either as antimicrobial agents, lubricant, and additives. The essence and importance of ILs is continuously being evaluated especially for the industrial applications and some ILs have not yet been synthesized or metathesized, and with their properties not fully evaluated. Herein, a series of novel imidazolium based ILs were synthesized using the metathesis method and characterized to determine their acidity, moisture, and thermal stability. Their physicochemical properties showed that [1-allyl-3-methylimidazolium] [TFO] is the most thermally stable compound according to the DSC/TGA analysis, and Karl Fisher analysis was used to determine the content of water. The acidity levels of the ILs determined by using a Hammett’s Acidity function revealed Ho values in the range of 3.9861 to 4.2857. The photocatalytic degradation process revealed that 1-allyl-3-methylimidazolium salicylate [Amim] [SaL] was still present after 72 hours. Microbial susceptibility testing of the ILs against selected Gram-positive and Gram-negative bacteria using agar well diffusion method revealed diverse degrees of susceptibility of the test organisms, with Pseudomonas aeruginosa ATCC BAA 1744 and Escherichia coli ATCC 11775 being the least and most sensitive to all the treatments, respectively.