bluuebunny/crossref_metadata_2025_split huggingface dataset:
Crystal structure of an S‐formylglutathione hydrolase from Pseudoalteromonas haloplanktis TAC125
Authors: Alterio Vincenzo , Aurilia Vincenzo , Romanelli Alessandra , Parracino Antonietta , Saviano Michele , D'Auria Sabato , De Simone Giuseppina 
Published: March 2010

AbstractS‐formylglutathione hydrolases (FGHs) constitute a family of ubiquitous enzymes which play a key role in formaldehyde detoxification both in prokaryotes and eukaryotes, catalyzing the hydrolysis of S‐formylglutathione to formic acid and glutathione. While a large number of functional studies have been reported on these enzymes, few structural studies have so far been carried out. In this article we report on the functional and structural characterization of PhEst, a FGH isolated from the psychrophilic bacterium Pseudoalteromonas haloplanktis. According to our functional studies, this enzyme is able to efficiently hydrolyze several thioester substrates with very small acyl moieties. By contrast, the enzyme shows no activity toward substrates with bulky acyl groups. These data are in line with structural studies which highlight for this enzyme a very narrow acyl‐binding pocket in a typical α/β‐hydrolase fold. PhEst represents the first cold‐adapted FGH structurally characterized to date; comparison with its mesophilic counterparts of known three‐dimensional structure allowed to obtain useful insights into molecular determinants responsible for the ability of this psychrophilic enzyme to work at low temperature. © 2010 Wiley Periodicals, Inc. Biopolymers 93: 669–677, 2010.This article was originally published online as an accepted preprint. The “Published Online” date corresponds to the preprint version. You can request a copy of the preprint by emailing the Biopolymers editorial office at biopolymers@wiley.com
Web laboratory for computer network
Authors: Jovanovic Nenad , Popovic Ranko , Markovic Suzana , Jovanovic Zoran 
Published: March 2010

AbstractCurrent technologies give us the ability to enhance and replace developmental classes with computer‐based resources, often called Web laboratories. Web laboratories have become a very useful support for practical aspects of teaching methods. This article presents a Web‐based laboratory, which makes learning of base principles of computer network possible. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 20: 493–502, 2012
ChemInform Abstract: Infrared Luminescence of Tm3+‐Doped Chalcohalide Glasses in GeS2—In2Se3—CsBr System.
Authors: Wang Wei , Xu Yinsheng , Shen Ce , Yan Qiqi , Zeng Huidan , Chen Guorong 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: La0.7Ca0.3MnO3 Perovskite Synthesized by Reactive Milling Method: The Effect of Particle Size on the Magnetic and Electrical Properties.
Authors: Manh D. H. , Phong P. T. , Thanh T. D. , Hong L. V. , Phuc N. X. 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Primary Amine‐Metal Lewis Acid Bifunctional Catalysts: The Application to Asymmetric Direct Aldol Reactions.
Authors: Xu Zhenghu , Daka Philias , Wang Hong 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Axial Chirality Control During Suzuki—Miyaura Cross‐Coupling Reactions: The tert‐Butylsulfinyl Group as an Efficient Chiral Auxiliary.
Authors: Colobert Francoise , Valdivia Victoria , Choppin Sabine , Leroux Frederic R. , Fernandez Inmaculada , Alvarez Eleuterio , Khiar Noureddine 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Chiral Phosphoric Acid Catalyzed Desymmetrization of meso‐Aziridines with Functionalized Mercaptans.
Authors: Larson Shawn E. , Baso Juan C. , Li Guilong , Antilla Jon C. 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The Au(I)‐Catalyzed Intramolecular Hydroarylation of Terminal Alkynes under Mild Conditions: Application to the Synthesis of 2H‐Chromenes, Coumarins, Benzofurans, and Dihydroquinolines.
Authors: Menon Rajeev S. , Findlay Alison D. , Bissember Alex C. , Banwell Martin G. 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Silver(I)‐Catalyzed Dual Activation of Propargylic Alcohol and Aziridine/Azetidine: Triggering Ring‐Opening and endo‐Selective Ring‐Closing in a Cascade.
Authors: Bera Milan , Roy Sujit 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Cupric Chloride‐Catalyzed Synthesis of Symmetrical Azo Compounds from Primary Aromatic Amines.
Authors: Zhang Ming , Zhang Rongli , Zhang Ai‐Qin , Li Xinfei , Liang Haihua 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Time‐Dependent Surface Plasmon Resonance Spectroscopy of Silver Nanoprisms in the Presence of Halide Ions
Authors: Hsu Ming‐Sheng , Cao Yi‐Wei , Wang Hsuang‐Wen , Pan Ya‐Sin , Lee Bo‐Hong , Huang Cheng‐Liang 
Published: March 2010

AbstractHerein, we find that the surface plasmon resonance (SPR) spectra of silver nanoprisms in the presence of halide ions change gradually with reaction time. The changes in the spectra correspond to the shape transformation of silver nanoprisms. There are threshold concentrations of halide ions that initiate the shape‐transformation reaction. The threshold concentrations for Cl−, Br−, and I− are about 3×10−4 M, 1×10−6 M, and 1.5×10−6 M, respectively. Any concentrations of the added halide ions above these thresholds can eventually etch the silver nanoprisms into nanodisks if the reaction time is long enough. The higher the concentration of the halide ions, the higher the etching rate will be. The kinetics of the shape transformation of the silver nanoprisms can be studied by recording their time‐dependent surface plasmon resonance (SPR) spectra on a commercial UV/Vis–NIR spectrometer. The peak positions of in‐plane dipole SPR bands of silver colloids in the presence of chloride and bromide ions can be fitted very well with the biexponential functions. We propose that the fast components of the biexponential behaviors should correlate to the truncating effect on the corners of silver nanoprisms, and the slow component should correlate to the redeposition of the truncated residues onto the basal plane of the nanoplates.
An efficient e‐mail protocol providing perfect forward secrecy for mobile devices
Authors: Chang Chin‐Chen , Lee Chia‐Yin , Chiu Yen‐Chang 
Published: March 2010

AbstractWith the explosive growth of Internet applications, e‐mail has become a popular medium for various types of data transmission. To ensure the confidentiality of an e‐mail, many secure e‐mail protocols have been proposed in recent years. In 2005, Sun et al. proposed an e‐mail protocol that claimed to have perfect forward secrecy. However, Dent pointed out some flaws of this protocol. According to Dent's comments, Kim et al. proposed two e‐mail protocols to overcome these weaknesses. In this article, we propose an efficient and secure e‐mail protocol that provides perfect forward secrecy. Our protocol is more suitable for mobile devices which have limited battery energy. Copyright © 2010 John Wiley & Sons, Ltd.
Frequent occurrence of high affinity T cells against MELOE‐1 makes this antigen an attractive target for melanoma immunotherapy
Authors: Godet Yann , Desfrançois Juliette , Vignard Virginie , Schadendorf Dirk , Khammari Amir , Dreno Brigitte , Jotereau Francine , Labarrière Nathalie 
Published: March 2010

AbstractWe recently showed that the infusion of tumor infiltrating lymphocytes specific for the MELOE‐1 antigen was associated with a prolonged relapse‐free survival for HLA‐A2+ melanoma patients who received tumor infiltrating lymphocytes therapy. Here, we characterized the MELOE‐1/A2‐specific T‐cell repertoire in healthy donors and melanoma patients to further support an immunotherapy targeting this epitope. Using tetramer enrichment followed by multicolor staining, we found that MELOE‐1‐specific T cells were present in the blood of healthy donors and patients at similar frequencies (around 1 in 1×105 CD8+ cells). These cells mainly displayed a naïve phenotype in 4/6 healthy donors and 3/6 patients, whereas high proportions of memory cells were observed in the remaining individuals of both groups. There was a recurrent usage of the Vα12.1 chain for 17/18 MELOE‐1‐specific T‐cell clones derived from healthy donors or patients, associated with diverse Vβ chains and V(D)J junctional sequences. All clones derived from melanoma patients (9/9) were reactive against the MELOE‐136–44 peptide and against HLA‐A2+ melanoma cell lines. This study documents the existence of a large TCR repertoire specific for the MELOE‐1/A2 epitope and its capacity to give rise to antitumor CTL that supports the development of immunotherapies targeting this epitope.
Silicon‐Assisted Direct Covalent Grafting on Metal Oxide Surfaces: Synthesis and Characterization of Carboxylate N,N′‐Ligands on TiO2
Authors: Arzoumanian Henri , Castellanos Nelson J. , Martínez Fernando O. , Páez‐Mozo Edgar A. , Ziarelli Fabio 
Published: March 2010

AbstractThe easy covalent bonding of an OH‐bearing molecule onto a metal oxide surface can be done by transesterifying a trimethylsilylated hydroxy function with the surface OH groups. This results in the grafting of the organic molecule directly on the matrix, accompanied by the formation of trimethylsilanol, which can easily be eliminated as volatile hexamethyldisiloxane and water. This was accomplished on a TiO2 matrix with three carboxylic acids: acetic, isonicotinic, and 2,2′‐bipyridyl‐4,4′‐dicarboxylic acids. The N,N′‐immobilized ligand was then used for a dioxidomolybdenum entity and tested as such at room temperature and under atmospheric pressure O2 oxidation of ethylbenzene. All intermediates and grafted species were fully characterized by 13CMAS NMR spectroscopy, and thermogravimetric and elemental analysis.
1D Chains Constructed from Oxido‐Centered [Mn3O] Units Exhibiting Single‐Chain Magnet Behavior
Authors: Song Xueyan , Yang PeiPei , Mei Xuelan , Li Licun , Liao Daizheng 
Published: March 2010

AbstractThe synthesis, crystal structure, and magnetic properties of two new MnIII single‐chain magnets, [Mn3O(L)3(HCOO)(CH3OH)4]·2CH3OH (1) and [Mn3O(L)3N3(C2H5OH)3(H2O)] (2) (H2L = 3‐tert‐butyl‐5‐methylsalicylaldoxime) are reported. Complexes 1 and 2 consist of a triangle of MnIII ions with an oxido‐center bridge and three 3‐tert‐butyl‐5‐methylsalicylaldoxime ligands that form a plane with the metal ions. Both complexes contain the same cores with the general formula [Mn3(μ3‐O)(L)3]+, which are further connected by formate (complex 1) or azide (complex 2) ligands into chain structures. Temperature‐dependent magnetic susceptibility studies indicate the presence of antiferromagnetic interactions between the MnIII ions in 1, whereas predominantly ferromagnetic interactions are observed in 2. Both complexes show slow relaxation of their magnetizations associated with single‐chain magnet behavior.
Titanacalixarenes in Homogeneous Catalysis: Synthesis, Conformation and Catalytic Activity in Ethylene Polymerisation (Eur. J. Inorg. Chem. 9/2010)
Authors: Espinas Jeff , Darbost Ulrich , Pelletier Jeremie , Jeanneau Erwan , Duchamp Christian , Bayard François , Boyron Olivier , Broyer Jean‐Pierre , Thivolle‐Cazat Jean , Basset Jean‐Marie , Taoufik Mostafa , Bonnamour Isabelle 
Published: March 2010

AbstractThe cover picture shows that improved titanium catalysts based on calixarene ligands can display high activity for the production of ultra‐high‐molecular‐weight polyethylene (UHMWPE). Both the coordination mode of the titanium and the conformation of the calixarene ligand have been found to be significant for the catalytic performance. Details are discussed in the article by M. Taoufik, I. Bonnamour et al. on p. 1349 ff.
3D Monodisperse Oligofluorenes with Non‐Conjugated Triphenylamine‐Based Cores: Synthesis and Optoelectronic Properties
Authors: Zhang Xiaomo , Quan Yiwu , Cui Zhe , Chen Qingmin , Ding Jianfu , Lu Jianping 
Published: March 2010

AbstractTwo 3D monodisperse oligofluorenes with non‐conjugated triphenylamine‐based cores have been synthesized byFriedel–Crafts copolycondensation reaction. The oligomers, PF3‐TPA and PF3‐TPA3, consist of three fluorene pentamer arms that are connected non‐conjugately through a triphenylamine (TPA) and 1,3,5‐tris(triphenylamino)benzene core (TPA3), respectively, at the 9‐position of the central fluorene of the pentafluorene arms. The coplanar structures ofthe cores and the linkages at the centre of the pentafluorene arms produced a 3D structure of oligomers. This specific structure efficiently retarded the crystallization tendencies of the pentafluorene arms and gave the materials completely amorphous morphological structures. Both oligomers emit deep‐blue fluorescence with high efficiencies in thin films (φpl–film = 67 % for PF3‐TPA3 and 86 % for PF3‐TPA). The introduction of triphenylamine units into the core promoted the hole‐injection ability while not obviously scarificing the electron‐injection ability of the oligomers. The multi‐layer devices ITO/PEDOT‐PSS/PF3‐TPA3 and PF3‐TPA/TPBI/LiF/Al were fabricated to investigate the electroluminescence (EL) properties of the two oligomers. Both oligomers showed a low turn‐on voltage of 4 V. The luminances reached 1946 cd/m2 at 7.5 V in the PF3‐TPA3 device and 1055 cd/m2 at 8 V in the PF3‐TPA device. The EL efficiencies at this luminance were 1.63 and 1.57 cd/A, respectively.
Electrooxidation of Glucose at Nanoporous Gold Surfaces: Structure Dependent Electrocatalysis and Its Application to Amperometric Detection
Authors: Seo Bora , Kim Jongwon 
Published: March 2010

AbstractElectrooxidation of glucose is investigated at nanoporous gold (NPG) with controlled surface structures by applying different deposition charges during the formation of AgAu layers. As the deposition charge increases, the NPG surfaces exhibit smaller ligament/pore structures and the electrocatalytic oxidation of glucose becomes more effective. Voltammetric responses of NPG suggest that the electrocatalytic oxidation arises from the enrichment of (110) or (100) surface orientation of gold with higher deposition charges. The electrooxidation of glucose is retained at NPG surfaces with higher deposition charges in the presence of Cl−, which suggests possible applications to the amperometric glucose detection in biological samples.
Measurement of intracellular accumulation of anthracyclines in cancerous cells by direct injection of cell lysate in MEKC/LIF detection
Authors: Mbuna Julius , Kaneta Takashi , Imasaka Totaro 
Published: March 2010

AbstractAnthracyclines are chemotherapeutic drugs that are broadly used in the treatment of various types of solid cancers and leukemia. Herein, we report on a novel analytical method for intracellular accumulation of anthracyclines using MEKC/LIF detection. An aqueous separation system permitted the injection of cell lysates directly into the capillary. The MEKC migrating solution was made up of borate buffer at pH 9.22 containing sodium taurodeoxycholate, (2‐hydroxypropyl)‐γ‐CD, and SDS. The anthracyclines, Doxorubicin (DOX) and epirubicin (EPI) were detected by LIF using a Nd:YAG laser (532 nm) or an argon ion laser (488 nm) for excitation. Two cell lines, human humerus tumor cells (RDES) and human lung tumor cells (A549), were treated with a mixture of the two anthracyclines for fixed periods of time, and then intracellular concentrations were determined by injecting cell lysates directly. Recovery values of 96.0–100.8% were obtained for DOX and EPI. Reproducibility quantified by RSD was less than 3.9% intraday and 6.7% interday at concentrations ranging between 50 and 500 nM. The uptake of EPI was found to be slightly less than that of DOX for A549, but higher levels of EPI were observed in RDES. Intracellular accumulation of anthracyclines was greater in RDES than in A549, but both types of cells excreted anthracyclines after 12 h. These results indicate that MEKC with an aqueous medium is useful for investigating intracellular uptake and accumulation of drugs, since cell lysates can be used directly with no pretreatment such as deproteination or solvent extraction of analytes.
Are there limits to collectivism? Culture and children's reasoning about lying to conceal a group transgression
Authors: Sweet Monica A. , Heyman Gail D. , Fu Genyue , Lee Kang 
Published: March 2010

AbstractThis study explored the effects of collectivism on lying to conceal a group transgression. Seven‐, 9‐, and 11‐year‐old US and Chinese children (N=374) were asked to evaluate stories in which protagonists either lied or told the truth about their group's transgression and were then asked about either the protagonist's motivations or justification for their own evaluations. Previous research suggests that children in collectivist societies such as China find lying for one's group to be more acceptable than do children from individualistic societies such as the United States. The current study provides evidence that this is not always the case: Chinese children in this study viewed lies told to conceal a group's transgressions less favourably than did US children. An examination of children's reasoning about protagonists' motivations for lying indicated that children in both countries focused on an impact to self when discussing motivations for protagonists to lie for their group. Overall, results suggest that children living in collectivist societies do not always focus on the needs of the group. Copyright © 2010 John Wiley & Sons, Ltd.
Metastasis suppressor function of NM23‐H1 requires its 3′‐5′ exonuclease activity
Authors: Zhang Qingbei , McCorkle Joseph R. , Novak Marian , Yang Mengmeng , Kaetzel David M. 
Published: March 2010

AbstractThe metastasis suppressor NM23‐H1 possesses 3 enzymatic activities in vitro, a nucleoside diphosphate kinase (NDPK), a protein histidine kinase and a more recently characterized 3′‐5′ exonuclease. Although the histidine kinase has been implicated in suppression of motility in breast carcinoma cell lines, potential relevance of the NDPK and 3′‐5′ exonuclease to metastasis suppressor function has not been addressed in detail. To this end, site‐directed mutagenesis and biochemical analyses of bacterially expressed mutant NM23‐H1 proteins have identified mutations that disrupt the 3′‐5′ exonuclease alone (Glu5 to Ala, or E5A), the NDPK and histidine kinase activities tandemly (Y52A, H118F) or all 3 activities simultaneously (K12Q). Although forced expression of NM23‐H1 potently suppressed spontaneous lung metastasis of subcutaneous tumor explants derived from the human melanoma cell line 1205LU, no significant metastasis suppressor activity was obtained with the exonuclease‐deficient variants E5A and K12Q. The H118F mutant, which lacked both the NDPK and histidine kinase while retaining the 3′‐5′ exonuclease, also exhibited compromised suppressor activity. In contrast, each mutant retained the ability to suppress motility and invasive characteristics of 1205LU cells in culture, indicating that the NM23‐H1 molecule possesses an additional activity(s) mediating these suppressor functions. These studies provide the first demonstration that the 3′‐5′ exonuclease activity of NM23‐H1 is necessary for metastasis suppressor function and further indicate cooperativity of the 3 enzymatic activities of the molecule on suppression of the metastatic process.
Rab4 interacts with the human P‐glycoprotein and modulates its surface expression in multidrug resistant K562 cells
Authors: Ferrándiz‐Huertas Clotilde , Fernández‐Carvajal Asia , Ferrer‐Montiel Antonio 
Published: March 2010

AbstractP‐glycoprotein (P‐gp) is a plasma membrane glycoprotein that has been signaled as a primary cause of multidrug resistance (MDR) in tumors. We performed a yeast 2‐hybrid screen using the C‐terminal domain of P‐gp and identified 2 small GTPases involved in vesicular trafficking, Rab4 and Rab14, which complex with P‐gp. The overexpression of GFP‐Rab4, either transiently or stably, but not of Rab14, in K562ADR cells decreased the presence of P‐gp in the cell surface. As a result, expression of this GTPase reduced the MDR phenotype of K562ADR cells, by augmenting the intracellular accumulation of daunomycin (DNM). This effect was mimicked by the constitutively active Rab4Q72L mutant, but not by the dominant negative Rab4S27N mutant. Rab4 regulated excocytotic P‐gp trafficking to the plasma membrane from intracellular compartments, and this modulation required the interaction of both proteins and the GTPase activity. Noteworthy, K562ADR cells exhibited a significant reduction of Rab4 levels, but not of other Rab GTPases, as compared with the sensitive parental cell line, suggesting that the development of the MDR phenotype in these cells involves upregulation of P‐gp and a concomitant downregulation of proteins that regulate its surface expression. Attenuation of endogenous Rab4 levels in K562ADR by RNA interference enhanced the expression of P‐gp in the cell surface, and reduced the uptake of DNM. Accordingly, these findings substantiate the notion that modulation of the temporal and spatial distribution of P‐gp in cancer cells may be a valid therapeutic strategy to alleviate the MDR phenotype, and signal to Rab4 as a potential target.