bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characteristics of fermentation and bioavailability of isoflavones in Korean soybean paste (doenjang) with application of Bacillus sp. KH‐15
Authors: Choi Yang Mun , Kim Yoon Sook , Ra Kyung Soo , Suh Hyung Joo 
Published: July 2010

SummaryWe attempted to increase the fibrinolytic activity and aglycone contents of isoflavone in doenjang prepared with Bacillus sp. KH‐15. An initial rapid increase of viable cells was observed in the first 30 days followed by a gradual decrease to 1.78 × 108 CFU g−1 wet weight. The amino type nitrogen sharply was increased after 15 days, and showed the highest level (788.7 mg%) after 90 days. The total nitrogen was increased from 2.21% to 2.52% of final content after 90 days. Soluble carbohydrate was increased to 32.9 mg g−1 for 60 days, and after 60 days, the content was slowly decreased to 29.5 mg g−1. A rapid increase in fibrinolytic and caseinolytic activities were observed in the first 30 and 45 days of fermentation followed by a gradual decrease to 2.54 and 144.5 U g−1, respectively. β‐Glucosidase activity also showed a similar change pattern of the caseinolytic and fibrinoytic activities. Daidzein and genistein were increased to the maximum level of 764.5 and 561.4 mg kg−1 at day 30, respectively. At day 90, the contents of daidzein and genistein were 661.5 and 524.9 mg kg−1, respectively. The rats were fed a diet supplemented with either doenjang powder prepared with Bacillus sp. KH‐15 (D‐1 group) or commercial doenjang powder (D‐2 group) for 40 days. The level of serum total isoflavones in the D‐1 group was significantly higher than that in the D‐2 group.
Cohort variation, climate effects and population dynamics in a short‐lived lizard
Authors: Le Galliard Jean François , Marquis Olivier , Massot Manuel 
Published: July 2010

Summary 1. Demographic theory and empirical studies indicate that cohort variation in demographic traits has substantial effects on population dynamics of long‐lived vertebrates but cohort effects have been poorly investigated in short‐lived species. 2. Cohort effects were quantified in the common lizard (Zootoca vivipara Jacquin 1787), a short‐lived ectothermic vertebrate, for body size, reproductive traits and age‐specific survival with mark–recapture data collected from 1989 to 2005 in two wetlands. We assessed cohort variation and covariation in demographic traits, tested the immediate and delayed effects of climate conditions (temperature and rainfall), and predicted consequences for population growth. 3. Most demographic traits exhibited cohort variation, but this variation was stronger for juvenile growth and survival, sub‐adult survival and breeding phenology than for other traits. 4. Cohort variation was partly explained by a web of immediate and delayed effects of climate conditions. Rainfall and temperature influenced distinct life‐history traits and the periods of gestation and early juvenile life were critical stages for climate effects. 5. Cohort covariation between demographic traits was usually weak, apart from a negative correlation between juvenile and sub‐adult body growth suggesting compensatory responses. An age‐structured population model shows that cohort variation influences population growth mainly through direct numerical effects of survival variation early in life. 6. An understanding of cohort effects is necessary to predict critical life stages and climatic determinants of population dynamics, and therefore demographic responses to future climate warming.
Inosine 5′‐monophosphate dehydrogenase 1 haplotypes and association with mycophenolate mofetil gastrointestinal intolerance in pediatric heart transplant patients
Authors: Ohmann Erin L. , Burckart Gilbert J. , Chen Yan , Pravica Vera , Brooks Maria M. , Zeevi Adriana , Webber Steven A. 
Published: July 2010

Ohmann EL, Burckart GJ, Chen Y, Pravica V, Brooks MM, Zeevi A, Webber SA. Inosine 5′‐monophosphate dehydrogenase 1 haplotypes and association with mycophenolate mofetil gastrointestinal intolerance in pediatric heart transplant patients. Pediatr Transplantation 2010: 14: 891–895. © 2010 John Wiley & Sons A/S.Abstract:  MMF, the most commonly used adjuvant immunosuppressant in pediatric heart transplantation, has frequent GI adverse events. SNPs in inosine 5′‐monophosphate dehydrogenase I (IMPDH1) may contribute to MMF GI intolerance. Phased haplotypes may have more utility than individual SNPs in candidate gene association studies for complex traits. This study defined common IMPDH1 haplotypes and investigated whether these haplotypes influence MMF GI intolerance in 59 pediatric heart recipients. Genotypes were assessed by Taqman analysis of IMPDH1 rs2288553, rs2288549, rs2278293, rs2278294, and rs2228075, and haplotypes were inferred using Arlequin 3.01 software. GI intolerance was defined as diarrhea, vomiting, nausea, or abdominal pain requiring MMF dose holding for > 48 h or MMF discontinuation. GI intolerance occurred in 21 patients (35.6%). Ten IMPDH1 haplotypes were identified in this population. In univariable analyses, one haplotype was strongly associated with MMF GI intolerance with 59.1% of carriers of this haplotype experiencing MMF GI intolerance compared to 21.6% of non‐carriers (p = 0.005). In this study, we identify a common IMPDH1 haplotype associated with MMF GI intolerance in a population of pediatric heart transplant patients. This haplotype of interest did not demonstrate stronger association with MMF GI intolerance than an individual IMPDH1 SNP.
Inhaled iloprost to control pulmonary artery hypertension in patients undergoing mitral valve surgery: a prospective, randomized‐controlled trial
Authors: REX S. , SCHAELTE G. , METZELDER S. , FLIER S. , DE WAAL E. E. C. , AUTSCHBACH R. , ROSSAINT R. , BUHRE W. 
Published: July 2010

Background: Pulmonary hypertension (PHT) is common in patients undergoing mitral valve surgery and is an independent risk factor for the development of acute right ventricular (RV) failure. Inhaled iloprost was shown to improve RV function and decrease RV afterload in patients with primary PHT. However, no randomized‐controlled trials on the intraoperative use of iloprost in cardiac surgical patients are available. We therefore compared the effects of inhaled iloprost vs. intravenous standard therapy in cardiac surgical patients with chronic PHT.Methods: Twenty patients with chronic PHT undergoing mitral valve repair were randomized to receive inhaled iloprost (25 μg) or intravenous nitroglycerine. Iloprost was administered during weaning from cardiopulmonary bypass (CPB). Systemic and pulmonary haemodynamics were assessed with pulmonary artery catheterization and transoesophageal echocardiography. Milrinone and/or inhaled nitric oxide were available as rescue medication in case of failure to wean from CPB.Results: Inhaled iloprost selectively decreased the pulmonary vascular resistance index after weaning from CPB (208 ± 108 vs. 422 ± 62 dyn·s/cm5/m2, P<0.05), increased the RV‐ejection fraction (29 ± 3% vs. 22 ± 5%, P<0.05), improved the stroke volume index (27 ± 7 vs. 18 ± 6 ml/m2, P<0.05) and reduced the transpulmonary gradient (10 ± 4 vs. 16 ± 3 mmHg, P<0.05). In all patients receiving inhaled iloprost, weaning from CPB was successful during the first attempt. In contrast, three patients in the control group required re‐institution of CPB and had to be weaned from CPB using rescue medication.Conclusions: In patients with pre‐existing PHT undergoing mitral valve surgery, inhaled iloprost is superior to intravenous nitrogylycerine by acting as a selective pulmonary vasodilator, reducing RV afterload and moderately improving RV‐pump performance.
Resolution of the nirD Locus for Heme d1, Synthesis of Cytochrome cd1, (Respiratory Nitrite Reductase) from Pseudomonas Stutzeri
Authors: Palmedo Gabriele , Seither Peter , Körner Heinz , Matthews Jeffrey C. , Burkhalter Robert S. , Timkovich Russell , Zumft Walter G. 
Published: July 2010

The genetic organization of the nirD locus of Pseudomonas stutzeri ZoBell, necessary for a catalytically active cytochrome cd1, (EC 1.9.3.2), was determined. The locus comprises the unidirectionally transcribed open reading frames nirFDLGH, downstream of nirMC of the nir gene cluster, and immediately upstream of the norCB operon encoding nitric oxide (NO) reductase (EC 1.7.99.7). Notable sequence relatedness was found between NirF and cytochrome cd1, (NirS), within NirDLGH, and between NirM and NirC, suggesting several gene duplication events in this region. The derived NirF protein (391 amino acids, Mr 43137) has 23.8% identity (51.1 % overall similarity) with NirS, but lacks the N‐terminal heme‐c ‐binding domain of NirS.Insertional mutagenesis of the five open reading frames resulted in the loss of respiratory nitrite reductase activity in vivo and in vitro. Mutant strains, when induced with nitrate for denitrification, synthesized a periplasmic cytochrome cd1 lacking heme d1 The defect was caused by the inability of the cell to synthesize heme d1 The nirD locus is proposed to encode a multimeric and multifunctional enzyme complex involved in the synthesis of heme d1.Mutations in nirFDLGH lowered substantially the expression level of norCB. Nir‐ mutants, unable to generate NO in vivo, provide indirect evidence for an NO sensor and an inducer role of NO for its cognate reductase.
Heterosis and combining ability for resistance to powdery mildew in adult melon plants
Authors: Shashikumar Kadanapuradadoddi T. , Pitchaimuthu Mottaiyan , Kumar Doddegowda P. , Rawal Ram D. 
Published: July 2010

With 4 tables AbstractTo estimate the heterosis, general combining ability (GCA) and specific combining ability (SCA) for resistance to powdery mildew, 30 F1 hybrids developed in a 6 × 5 line × tester mating design were evaluated both under the greenhouse and field conditions. Predominance of non‐additive variance over additive variance in the inheritance of resistance to powdery mildew was detected. Inbred lines IIHR 681, IIHR 352, IIHR 190, RM 43, IIHR 121 and IIHR 122 demonstrated consistently high and negative GCA effects. There was a strong correlation between GCA effect and mean per cent disease index (PDI) of parent that would permit the use of the mean performance of the parental lines to predict the value of a resulting hybrid. Six hybrids, that is, Arka Jeet × IIHR 122, Punjab Sunehri × IIHR 190, RM 43 × IIHR 121, IIHR 681 × IIHR 616, IIHR 352 × IIHR 616 and IIHR 352 × IIHR 718 which involved one or both the parents with good GCA effects exhibited low mean PDI with negative mid‐parent heterosis and negative SCA effects.
Construct validity and reliability of the Practice Environment Scale of the Nursing Work Index for Queensland nurses
Authors: Parker Deborah , Tuckett Anthony , Eley Robert , Hegney Desley 
Published: July 2010

Parker D, Tuckett A, Eley R, Hegney D.International Journal of Nursing Practice2010;16: 352–358 Construct validity and reliability of the Practice Environment Scale of the Nursing Work Index for Queensland nursesThis article reports on construct validity and reliability of 30 items of the Practice Environment Scale of the Nursing Work Index (PES‐NWI). Australia, like other countries, is experiencing a shortage of nurses; a multifactor approach to retention of nurses is required. One significant factor that has received increasing attention in the last decade, particularly in the USA, is the nursing practice environment. The reliability of the 30 items of the PES‐NWI was assessed by Cronbach's alpha and factor analysis was performed using principal component analysis. The PES‐NWI was completed by nurses working in the aged‐care, private and public sectors in Queensland, Australia. A total of 3000 surveys were distributed to a random sample of members of the Queensland Nurses Union. Of these, 1192 surveys were returned, a response rate of 40%. The PES‐NWI was shown to be reliable demonstrating internal consistency with a Cronbach's alpha of the total scale of 0.948. The 30 items loaded onto five factors explaining 57.7% of the variance. The items across the factors differed slightly from those reported by the original author of the PES‐NWI. This study indicates that the PES‐NWI has construct validity and reliability in the Australian setting for nurses.
Self‐compassion and emotional intelligence in nurses
Authors: Heffernan Mary , Quinn Griffin Mary T , McNulty Sister Rita , Fitzpatrick Joyce J 
Published: July 2010

Heffernan M, Quinn Griffin MT, McNulty SR, Fitzpatrick JJ. International Journal of Nursing Practice 2010; 16: 366–373 Self‐compassion and emotional intelligence in nursesNurses often provide care for patients and families who are suffering and where emotions are heightened. Compassion is an essential component of the care that nurses provide. Emotions play an important role in the relationship and communication between nurses, patients and families. Self‐compassion is the ability to be compassionate to oneself, without this ability nurses might not be prepared to be compassionate to patients. Emotionally intelligent persons perceive themselves as confident, better able to understand, control and manage their emotions. The purpose of this descriptive, correlational study was to examine the relationship between self‐compassion and emotional intelligence. Participants were 135 nurses. The setting for this study was a health system with hospitals located in Queens, Nassau and Suffolk counties of New York, USA. Three of the hospitals in the study are located in Queens and/or the Queens/Nassau border. Queens is the most culturally diverse community in the USA. The patients served, as well as the nursing staff, are reflective of this cultural and religious diversity. Results indicated a positive correlation between self‐compassion and emotional intelligence (r = 0.55). Recommendations for future research include: exploration of self‐compassion and emotional intelligence in nurses, and identification of the benefits of enhancing self‐compassion and emotional intelligence in nurses.
Going home better not worse: Older adults' views on physical function during hospitalization
Authors: Boltz Marie , Capezuti Elizabeth , Shabbat Nina , Hall Kimberly 
Published: July 2010

Boltz M, Capezuti E, Shabbat N, Hall K. International Journal of Nursing Practice 2010; 16: 381–388 Going home better not worse: Older adults' views on physical function during hospitalizationFunctional decline is a common complication for hospitalized older adults. Illuminating the factors that influence the physical function of hospitalized older adults is critical in order to develop effective interventions to prevent avoidable loss of function. Twenty‐four older adults in three senior centres located in metropolitan New York City, who had recent experience with hospitalization, participated in focus groups to discover these factors. An exploratory qualitative design was used. Participants defined physical function as the ability to be mobile and resume the enactment of their roles, routines and relationships. Participants also believed that hospitalization should improve physical function. They described staff and system supports of, as well as the challenges to physical function in the hospital setting. The findings provide evidence for developing education programmes as well as new models of nursing care aimed at preventing functional decline.
Predictors of obesity in school‐aged Jordanian adolescents
Authors: Manal Ibrahim Al‐Kloub , Mousa Ali Al‐Hassan , Erika Sivarajan Froelicher 
Published: July 2010

Manal Ibrahim A‐K, Mousa Ali A‐H, Erika Sivarajan F. International Journal of Nursing Practice 2010; 16: 397–405 Predictors of obesity in school‐aged Jordanian adolescentsThis cross‐sectional study aimed to estimate the frequency of overweight and obesity in adolescents as defined by the International Obesity Task Force, and to estimate the effect of sociodemographic and health behaviours (eating habits and physical activity) that predict obesity. A stratified (by gender) random sample of 518 adolescents, aged 15 or 16 years was obtained from eight public schools in Amman. In this sample 17.5% were overweight and 9.6% were obese. The predictors of obesity and overweight (excess weight) were: (i) fathers attained primary and secondary education; (ii) total monthly family income ≥ 300 (JD); (iii) working mothers; (iv) family size ≤ 6; and (v) having obese parents. Eating a low quality diet (chips, candy) was a significant dietary predictor of excess weight. The family variables found to be important predictors along with a low quality diet suggest that family interventions would be necessary in the control of adolescent excess weight.
Some reflections on Curtis Prairie and the genesis of ecological restoration
Authors: Jordan William R. 
Published: July 2010

Summary  This article draws from the transcript of a talk given to Australian audience in Perth directly following the SERI conference in Western Australia 2009. It discusses Bill Jordan’s role in the genesis of the term ‘restoration ecology’ and the discipline of ‘ecological restoration’ whilst working at the University of Wisconsin‐Madison Arboretum.In 1977, Bill, a plant physiologist by training, was engaged by the Arboretum to develop a public outreach program. He felt that the Arboretum had a special story to tell as it was the site of the Depression‐era creation of a collection of restored ecological communities, which has since gained recognition as one of the earliest systematic attempts at what Dr Jordan and his colleague George Lubick have called ‘allocentric’– that is, other‐centered – ecological restoration. The centrepiece of that project, a 24‐ha prairie restoration on former cropping land and pasture now called the John T. Curtis Prairie dates back to an effort undertaken by a small group of scientists, including botanist Norman Fassett, ecologist Theodore Sperry and pioneering conservationist Aldo Leopold, and is now recognised internationally as a kind of locus classicus of this distinctive form of land management.Thinking hard about the meaning of this unique restoration project and its relationship to the University and to environmentalism, Bill and his colleague Keith Wendt coined the term ‘restoration ecology’ to describe the process of using restoration to field test ecological hypotheses. They also started the journal ‘Restoration & Management Notes’ (now ‘Ecological Restoration’) which, under Bill’s editorship, provided an influential early forum for the emerging discipline of ecological restoration.
Fourier transform infrared spectroscopy as a metabolite fingerprinting tool for monitoring the phenotypic changes in complex bacterial communities capable of degrading phenol
Authors: Wharfe Emma S. , Jarvis Roger M. , Winder Catherine L. , Whiteley Andrew S. , Goodacre Royston 
Published: July 2010

SummaryThe coking process produces great volumes of wastewater contaminated with pollutants such as cyanides, sulfides and phenolics. Chemical and physical remediation of this wastewater removes the majority of these pollutants; however, these processes do not remove phenol and thiocyanate. The removal of these compounds has been effected during bioremediation with activated sludge containing a complex microbial community. In this investigation we acquired activated sludge from an industrial bioreactor capable of degrading phenol. The sludge was incubated in our laboratory and monitored for its ability to degrade phenol over a 48 h period. Multiple samples were taken across the time‐course and analysed by Fourier transform infrared (FT‐IR) spectroscopy. FT‐IR was used as a whole‐organism fingerprinting approach to monitor biochemical changes in the bacterial cells during the degradation of phenol. We also investigated the ability of the activated sludge to degrade phenol following extended periods (2–131 days) of storage in the absence of phenol. A reduction was observed in the ability of the microbial community to degrade phenol and this was accompanied by a detectable biochemical change in the FT‐IR fingerprint related to cellular phenotype of the microbial community. In the absence of phenol a decrease in thiocyanate vibrations was observed, reflecting the ability of these communities to degrade this substrate. Actively degrading communities showed an additional new band in their FT‐IR spectra that could be attributed to phenol degradation products from the ortho‐ and meta‐cleavage of the aromatic ring. This study demonstrates that FT‐IR spectroscopy when combined with chemometric analysis is a very powerful high throughput screening approach for assessing the metabolic capability of complex microbial communities.
Denitrification at pH 4 by a soil‐derived Rhodanobacter‐dominated community
Authors: Van Den Heuvel R. N. , Van Der Biezen E. , Jetten M. S. M. , Hefting M. M. , Kartal B. 
Published: July 2010

SummarySoil denitrification is a major source of nitrous oxide emission that causes ozone depletion and global warming. Low soil pH influences the relative amount of N2O produced and consumed by denitrification. Furthermore, denitrification is strongly inhibited in pure cultures of denitrifying microorganisms below pH 5. Soils, however, have been shown to denitrify at pH values as low as pH 3. Here we used a continuous bioreactor to investigate the possibility of significant denitrification at low pH under controlled conditions with soil microorganisms and naturally available electron donors. Significant NO3‐ and N2O reduction were observed for 3 months without the addition of any external electron donor. Batch incubations with the enriched biomass showed that low pH as well as low electron donor availability promoted the relative abundance of N2O as denitrification end‐product. Molecular analysis of the enriched biomass revealed that a Rhodanobacter‐like bacterium dominated the community in 16S rRNA gene libraries as well as in FISH microscopy during the highest denitrification activity in the reactor. We conclude that denitrification at pH 4 with natural electron donors is possible and that a Rhodanobacter species may be one of the microorganisms involved in acidic denitrification in soils.
Transgenic poplar expressing Arabidopsis NDPK2 enhances growth as well as oxidative stress tolerance
Authors: Kim Yun‐Hee , Kim Myoung Duck , Choi Young Im , Park Sung‐Chul , Yun Dae‐Jin , Noh Eun Woon , Lee Haeng‐Soon , Kwak Sang‐Soo 
Published: July 2010

SummaryNucleoside diphosphate kinase 2 (NDPK2) is known to regulate the expression of antioxidant genes in plants. Previously, we reported that overexpression of Arabidopsis NDPK2 (AtNDPK2) under the control of an oxidative stress‐inducible SWPA2 promoter in transgenic potato and sweetpotato plants enhanced tolerance to various abiotic stresses. In this study, transgenic poplar (Populus alba × Poplus glandulosa) expressing the AtNDPK2 gene under the control of a SWPA2 promoter (referred to as SN) was generated to develop plants with enhanced tolerance to oxidative stress. The level of AtNDPK2 expression and NDPK activity in SN plants following methyl viologen (MV) treatment was positively correlated with the plant’s tolerance to MV‐mediated oxidative stress. We also observed that antioxidant enzyme activities such as ascorbate peroxidase, catalase and peroxidase were increased in MV‐treated leaf discs of SN plants. The growth of SN plants was substantially increased under field conditions including increased branch number and stem diameter. SN plants exhibited higher transcript levels of the auxin‐response genes IAA2 and IAA5. These results suggest that enhanced AtNDPK2 expression affects oxidative stress tolerance leading to improved plant growth in transgenic poplar.