bluuebunny/crossref_metadata_2025_split huggingface dataset:
Accelerometer-based sedentary time and physical activity from childhood through young adulthood with progressive cardiac changes: a 13-year longitudinal study
Authors: Agbaje Andrew O 
Published: May 2024

Abstract

Aims
Longitudinal evidence on the relationship of sedentary time (ST), light-intensity physical activity (LPA), and moderate-to-vigorous-intensity physical activity (MVPA) with changes in cardiac structure and function in the paediatric population is scarce. This evidence is clinically important due to the impact ST can have on the long-term prognosis of healthy young population in the lifetime continuum. This prospective observational study examined the relationships of cumulative ST, LPA, and MVPA from childhood with longitudinal changes in cardiac structure and function.

Methods and results
This is a secondary analysis from the Avon Longitudinal Study of Parents and Children, UK birth cohort of 1682 children aged 11 years. Participants who had at least one follow-up timepoints accelerometer-measured ST, LPA, and MVPA over a period of 13 years and repeated echocardiography-measured cardiac structure and function at ages 17- and 24-year clinic visit were included. Left ventricular mass indexed for height2.7 (LVMI2.7) and left ventricular (LV) diastolic function from mitral E/A ratio (LVDF) were computed. Among 1682 children (mean [SD] age, 11.75 [0.24] years; 1054 [62.7%] females), the cumulative one-min/day increase in ST from ages 11 to 24 years was associated with progressively increased LVMI2.7 {effect estimate 0.002 g/m2.7 [confidence interval (CI) 0.001–0.003], P &lt; 0.001}, irrespective of sex, obesity, and hypertensive status. Cumulative one-min/day increase in LPA was associated with a decreased LVMI2.7 (−0.005 g/m2.7 [−0.006 to −0.003], P &lt; 0.0001) but an increased LVDF. Cumulative one-minute/day increase in MVPA was associated with progressively increased LVMI2.7 (0.003 g/m2.7 [0.001–0.006], P = 0.015).

Conclusion
ST contributed +40% to the 7-year increase in cardiac mass, MVPA increased cardiac mass by +5%, but LPA reduced cardiac mass by −49%. Increased ST may have long-term pathologic effects on cardiac structure and function during growth from childhood through young adulthood; however, engaging in LPA may enhance cardiac health in the young population.
Exercise effects on maternal vascular health and blood pressure during pregnancy and postpartum: a systematic review and meta-analysis
Authors: Pongpanit Karan , Dayan Natalie , Janaudis-Ferreira Tania , Roig Marc , Spahija Jadranka , Bertagnolli Mariane 
Published: May 2024

Abstract

Aims
This systematic review aimed to assess the effects of exercise training during pregnancy and the postpartum period on maternal vascular health and blood pressure (BP).

Methods and results
The outcome of interest was pulse wave velocity (PWV), flow-mediated dilation (FMD), and BP from pregnancy to 1-year postpartum. Five databases, including Ovid MEDLINE, EMBASE, CINAHL, Web of Science, and Cochrane Library, were systematically searched from inception to August 2023. Studies of randomized controlled trials (RCTs) comparing the effects of prenatal or postpartum exercise to a non-exercise control group were included. The risk of bias and the certainty of evidence were assessed. Random-effects meta-analyses and sensitivity analyses were conducted. In total, 20 RCTs involving 1221 women were included. Exercise training, initiated from Week 8 during gestation or between 6 and 14 weeks after delivery, with the programme lasting for a minimum of 4 weeks up to 6 months, showed no significant impact on PWV and FMD. However, it resulted in a significant reduction in systolic BP (SBP) [mean difference (MD): −4.37 mmHg; 95% confidence interval (CI): −7.48 to −1.26; P = 0.006] and diastolic BP (DBP) (MD: −2.94 mmHg; 95% CI: −5.17 to −0.71; P = 0.01) with very low certainty. Subgroup analyses revealed consistent trends across different gestational stages, types of exercise, weekly exercise times, and training periods.

Conclusion
Exercise training during pregnancy and the postpartum period demonstrates a favourable effect on reducing maternal BP. However, further investigations with rigorous methodologies and larger sample sizes are needed to strengthen these conclusions.
Translational control of MPS1 links protein synthesis with the initiation of cell division and spindle pole body duplication in Saccharomyces cerevisiae
Authors: Blank Heidi M , Alonso Annabel , Fabritius Amy S , Valk Ervin , Loog Mart , Winey Mark , Polymenis Michael 
Published: May 2024

Abstract
Protein synthesis underpins cell growth and controls when cells commit to a new round of cell division at a point in late G1 of the cell cycle called Start. Passage through Start also coincides with the duplication of the microtubule-organizing centers, the yeast spindle pole bodies, which will form the 2 poles of the mitotic spindle that segregates the chromosomes in mitosis. The conserved Mps1p kinase governs the duplication of the spindle pole body (SPB) in Saccharomyces cerevisiae. Here, we show that the MPS1 transcript has a short upstream open reading frame (uORF) that represses the synthesis of Mps1p. Mutating the MPS1 uORF makes the cells smaller, accelerates the appearance of Mps1p in late G1, and promotes completion of Start. Monitoring the SPB in the cell cycle using structured illumination microscopy revealed that mutating the MPS1 uORF enabled cells to duplicate their SPB earlier at a smaller cell size. The accelerated Start of MPS1 uORF mutants depends on the G1 cyclin Cln3p and the transcriptional repressor Whi5p but not on the Cln1,2p G1 cyclins. These results identify growth inputs in mechanisms that control duplication of the microtubule-organizing center and implicate these processes in the coupling of cell growth with division.
Biography of Vitis genomics: recent advances and prospective
Authors: Wang Yi , Ding Kangyi , Li Huayang , Kuang Yangfu , Liang Zhenchang 
Published: May 2024

Abstract
The grape genome is the basis for grape studies and breeding, and is also important for grape industries. In the last two decades, more than 44 grape genomes have been sequenced. Based on these genomes, researchers have made substantial progress in understanding the mechanism of biotic and abiotic resistance, berry quality formation, and breeding strategies. In addition, this work has provided essential data for future pangenome analyses. Apart from de novo assembled genomes, more than six whole-genome sequencing projects have provided datasets comprising almost 5000 accessions. Based on these datasets, researchers have explored the domestication and origins of the grape and clarified the gene flow that occurred during its dispersed history. Moreover, genome-wide association studies and other methods have been used to identify more than 900 genes related to resistance, quality, and developmental phases of grape. These findings have benefited grape studies and provide some basis for smart genomic selection breeding. Moreover, the grape genome has played a great role in grape studies and the grape industry, and the importance of genomics will increase sharply in the future.
Is the African Vaccine Manufacturing Accelerator a decoupling mechanism?
Authors: Awosusi Abiodun E 
Published: May 2024

Abstract
This article explores how the African Vaccine Manufacturing Accelerator can support the sustainable production of vaccines in Africa. It highlights the value of the accelerator in relation to the Regional Vaccine Manufacturing Collaborative. The author proposes that this novel financing instrument should be well-designed and implemented in line with the targets of the Partnerships for African Vaccine Manufacturing. It should not be a decoupling tool to appease the institutional environment of the global vaccine market, but a sustainable demonstration of the goodwill and commitment of political and technical leaders to ensure equitable access to routine and epidemic-related vaccines in Africa.
Assessment of Preanalytical Cerebrospinal Fluid Handling and Storage Factors on Measurement of Aβ1-42, Aβ1-40, and pTau181 Using an Automated Chemiluminescent Platform
Authors: Ho Sara , Darrow Jacqueline , De Simone Francesca , Calabro Amanda , Gannon Sara , Esquivel Rianne , Thakker Parmi , Khingelova Kristina , Rao Aruna , Zhang Yifan , Moghekar Abhay 
Published: May 2024

Abstract

Background
Standardizing cerebrospinal fluid (CSF) laboratory protocols will improve the reliability and availability of clinical biomarker testing required for prescription of novel Alzheimer disease (AD) therapies. This study evaluated several preanalytical handling and storage factors common to β-amyloid1-42 (Aβ1-42), β-amyloid1-40 (Aβ1-40), and phosphorylated tau (pTau181) concentrations including storage at different temperatures, extended cap contact, various mixing methods, and multiple freeze-thaw cycles.

Methods
Aβ1-42, Aβ1-40, and pTau181 concentrations were measured using LUMIPULSE G1200 automated assays. Samples were collected in polypropylene tubes of various volumes. Sample cap-contact was evaluated by storing samples in upright and inverted positions at either 4°C for 1 week or −80°C for 1 month. To assess mixing methods, samples were freeze-thawed and mixed by inversion, vortex, horizontal roller, or unmixed prior to assay sampling. The impact of successive freeze-thaw cycles was assessed through freezing, thawing, and analyzing CSF samples.

Results
Short-term storage at 4°C did not affect Aβ1-42, Aβ1-40, or pTau181 measurements in any tube type. Tube cap contact affected Aβ1-42 in 2.5 mL tubes and pTau181 levels in 10 mL tubes. No difference was observed between mixing methods. After 4 freeze-thaw cycles, Aβ1-42 significantly decreased but Aβ1-40 remained unchanged. Utilizing the Aβ1-42/Aβ1-40 ratio, Aβ1-42 values normalized, maintaining ratio values within ±5% of baseline measurements.

Conclusions
Storage of CSF at 4°C for 1 week or −80°C for 1 month did not significantly affect Aβ1-42, Aβ1-40, pTau181, or associated ratio measurements. Tube cap-contact impacted pTau181 and pTau181/Aβ1-42 values in larger tubes. Mixing methods are equivalent. The Aβ1-42/Aβ1-40 ratio compensates for freeze-thaw variability up to 4 cycles.
Evaluating site-of-care-related racial disparities in kidney graft failure using a novel federated learning framework
Authors: Tong Jiayi , Shen Yishan , Xu Alice , He Xing , Luo Chongliang , Edmondson Mackenzie , Zhang Dazheng , Lu Yiwen , Yan Chao , Li Ruowang , Siegel Lianne , Sun Lichao , Shenkman Elizabeth A , Morton Sally C , Malin Bradley A , Bian Jiang , Asch David A , Chen Yong 
Published: May 2024

Abstract

Objectives
Racial disparities in kidney transplant access and posttransplant outcomes exist between non-Hispanic Black (NHB) and non-Hispanic White (NHW) patients in the United States, with the site of care being a key contributor. Using multi-site data to examine the effect of site of care on racial disparities, the key challenge is the dilemma in sharing patient-level data due to regulations for protecting patients’ privacy.

Materials and Methods
We developed a federated learning framework, named dGEM-disparity (decentralized algorithm for Generalized linear mixed Effect Model for disparity quantification). Consisting of 2 modules, dGEM-disparity first provides accurately estimated common effects and calibrated hospital-specific effects by requiring only aggregated data from each center and then adopts a counterfactual modeling approach to assess whether the graft failure rates differ if NHB patients had been admitted at transplant centers in the same distribution as NHW patients were admitted.

Results
Utilizing United States Renal Data System data from 39 043 adult patients across 73 transplant centers over 10 years, we found that if NHB patients had followed the distribution of NHW patients in admissions, there would be 38 fewer deaths or graft failures per 10 000 NHB patients (95% CI, 35-40) within 1 year of receiving a kidney transplant on average.

Discussion
The proposed framework facilitates efficient collaborations in clinical research networks. Additionally, the framework, by using counterfactual modeling to calculate the event rate, allows us to investigate contributions to racial disparities that may occur at the level of site of care.

Conclusions
Our framework is broadly applicable to other decentralized datasets and disparities research related to differential access to care. Ultimately, our proposed framework will advance equity in human health by identifying and addressing hospital-level racial disparities.
A role for miRNAs in the regulation of brown adipose tissue whitening in goats (Capra Hircus)
Authors: Zhao Le , Li Minhao , Xiao Min , Chen Meixi , Yang Haili , Zhao Yongju 
Published: May 2024

Abstract
A study of the mechanism of and metabolic regulation of brown adipose tissue (BAT) production is important for improving the survival rate of young animals. In the present study, we observed that perirenal adipose tissue in goats undergoes a rapid BAT whitening after birth. However, the underlying regulatory mechanism remains unknown. To address this further, we investigated the role of miRNAs in regulating the whitening process of BAT in goats. First, we identified the dynamic expression profiles of miRNAs during the whitening of BAT in Dazu black goat using RNA-seq. We identified a total of 1374 miRNAs, including 408 existing miRNAs, 693 known miRNAs, and 273 novel miRNAs. By analysis of the differentially expressed miRNAs (DE miRNAs), we found that 102 highly expressed miRNAs, including chi-miR-144-3p, chi-miR-144-5p, chi-miR-378-5p, chi-miR-136-3p, chi-miR-381, chi-miR-323b, chi-miR-1197-3p, chi-miR-411b-3p, and chi-miR-487a-3p, were enriched in BAT. In addition, 60 highly expressed miRNAs, including chi-miR-184, chi-miR-193a, chi-miR-193b-3p, chi-let-7c-5p, and chi-let-7e-5p, were enriched in white fat-like tissue. An analysis of miRNAs that were linearly downregulated (profile 0) or linearly upregulated (profile 19) over the D0—D28 period found that these DE miRNAs were mainly enriched in the Hippo signaling pathway, Cytokine-cytokine receptor interactions, and the TGF-beta signaling pathway. Furthermore, we confirmed that chi-let-7e-5p promotes the proliferation and differentiation of brown adipocytes. These results should facilitate a better understanding of the molecular regulation of miRNAs involved in BAT whitening in goats.
The Ten-Year Revolution
Authors: D’Alessandra Federica 
Published: May 2024

Abstract
This article reviews the evolution of the role of civil society documentation efforts in international justice proceedings since 2013 circa. It is written from my personal perspective by virtue of my involvement in a series of initiatives aimed to strengthen civil society’s (as well as other international justice actors’) documentation efforts through the collection of standards and best practices, which the essay also discusses. In particular, it reviews the process and challenges of setting forth the first comprehensive guidance and set of best practices for civil society documentation with the Public International Law and Policy Group’s ‘Handbook on Civil Society Documentation of Serious Human Rights Violations’. It argues that civil society documentation efforts have undergone a revolution within the field: from the margins to the very heart of international judicial proceedings upholding accountability for international crimes. This revolution was, in part, rendered possible by a changing documentation landscape and the proliferation of documentation resources and capabilities now readily available to the civil society. After reviewing the current state of play with respect to documentary efforts and documentation best practices, which I situate within the context of a broader evolution of the international justice ‘ecosystem’, the article addresses some ongoing challenges in documentation and areas that would benefit of further strengthening in the future.
Exploring the frontiers of therapeutic breadth of antifungal peptides: A new avenue in antifungal drugs
Authors: Ul Haq Ihtisham , Maryam Sajida , Shyntum Divine Y , Khan Taj A , Li Fan 
Published: May 2024

Abstract

The growing prevalence of fungal infections alongside rising resistance to antifungal drugs poses a significant challenge to public health safety. At the close of the 2000s, major pharmaceutical firms began to scale back on antimicrobial research due to repeated setbacks and diminished economic gains, leaving only smaller companies and research labs to pursue new antifungal solutions. Among various natural sources explored for novel antifungal compounds, antifungal peptides (AFPs) emerge as particularly promising. Despite their potential, AFPs receive less focus than their antibacterial counterparts. These peptides have been sourced extensively from nature, including plants, animals, insects, and especially bacteria and fungi. Furthermore, with advancements in recombinant biotechnology and computational biology, AFPs can also be synthesized in lab settings, facilitating peptide production. AFPs are noted for their wide-ranging efficacy, in vitro and in vivo safety, and ability to combat biofilms. They are distinguished by their high specificity, minimal toxicity to cells, and reduced likelihood of resistance development. This review aims to comprehensively cover AFPs, including their sources—both natural and synthetic—their antifungal and biofilm-fighting capabilities in laboratory and real-world settings, their action mechanisms, and the current status of AFP research.

One-Sentence Summary
This comprehensive review of AFPs will be helpful for further research in antifungal research.
T cell responses in immune-mediated IgA nephropathy
Authors: Xie Shimin , Sun Mengying , Zhang Xiaohan , Kan Chao , Shi Guojuan , Peng Weixiang , Guo Junli , Wu Dantong , Yin Zhinan , Yang Quanli , Zhang Rui 
Published: May 2024

Abstract
Immunoglobulin A nephropathy is a complex autoimmune disease with various underlying causes and significant clinical heterogeneity. There are large individual differences in its development, and the etiology and pathogenesis are still poorly understood. While it is known that immunobiological factors play a significant role in the pathophysiology of immunoglobulin A nephropathy, the specific nature of these factors has yet to be fully elucidated. Numerous investigations have verified that CD4+ and CD8+ T lymphocytes are involved in the immunopathogenesis of immunoglobulin A nephropathy. Furthermore, certain data also point to γδT cells’ involvement in the pathophysiology of immunoglobulin A nephropathy. By thoroughly examining the mechanisms of action of these T cells in the context of immunoglobulin A nephropathy, this review sheds light on the immunopathogenesis of the disease and its associated factors. The review is intended to provide reference value for the future research in this field and promising treatment clues for clinical patients.
Seasonal shifts in the habitat selection patterns of male American Marten (Martes americana) at a fine spatial scale
Authors: Viau Julie-Pier , Sigouin Daniel , St-Laurent Martin-Hugues 
Published: May 2024

Abstract
Old-growth forests harbor a large amount of complex structural features that result in a wide array of wildlife habitats. However, intensive forest management is gradually converting old-growth forest into younger, even-aged stands, reducing structural complexity and threatening the persistence of old-growth-dependent species. Maintaining elements of complex stand structure is critical to the conservation of old-growth forest specialists that use different habitat components at different periods of their annual cycle, and it requires a comprehensive understanding of seasonal variation in the habitat needs of these species. However, difficulties in observing free-ranging animals have sometimes limited our ability to assess such variations in habitat requirements, especially for small, elusive species. To address this, we used GPS telemetry collars to describe fine-scale habitat selection patterns of 6 male American Martens (Martes americana) during 2 contrasting periods of the year (snow-free, from mid-April to mid-November; snow-covered, from mid-November to mid-April), an objective formerly hard to achieve using conventional VHF telemetry. We used resource selection functions conducted at the fourth order of selection to compare habitat characteristics found at the sites used by martens (GPS locations, n = 100) to those found on an equal number of available sites (random points, n = 100) within each individual seasonal home range. We conducted vegetation surveys on these 200 sites to describe habitat and built candidate models representing different concurrent hypotheses. Our results showed that proxies of prey availability, predator avoidance, and thermal constraints were the primary factors influencing marten habitat selection during both periods, although their respective importance differed between periods. Martens selected sites with a high density of large-diameter snags (≥30·ha−1), high conifer canopy closure (≥53%), and a dense lateral cover (≥81%) during the snow-free period, but selected sites with a high volume of coarse woody debris (≥64 m3·ha−1) and high conifer canopy closure (≥48%) during the snow-covered period. Our results highlight the importance of contrasting seasonal changes in habitat selection patterns of small carnivores and may help maintain structural attributes in the landscape that are suitable for male American Martens.
Project Hephaistos – II. Dyson sphere candidates from Gaia DR3, 2MASS, and WISE
Authors: Suazo Matías , Zackrisson Erik , Mahto Priyatam K , Lundell Fabian , Nettelblad Carl , Korn Andreas J , Wright Jason T , Majumdar Suman 
Published: May 2024

ABSTRACT
The search for extraterrestrial intelligence is currently being pursued using multiple techniques and in different wavelength bands. Dyson spheres, megastructures that could be constructed by advanced civilizations to harness the radiation energy of their host stars, represent a potential technosignature, that in principle may be hiding in public data already collected as part of large astronomical surveys. In this study, we present a comprehensive search for partial Dyson spheres by analysing optical and infrared observations from Gaia, 2MASS, and WISE. We develop a pipeline that employs multiple filters to identify potential candidates and reject interlopers in a sample of five million objects, which incorporates a convolutional neural network to help identify confusion in WISE data. Finally, the pipeline identifies seven candidates deserving of further analysis. All of these objects are M-dwarfs, for which astrophysical phenomena cannot easily account for the observed infrared excess emission.