bluuebunny/crossref_metadata_2025_split huggingface dataset:
Biogenesis of MiRNA‐195 and its role in biogenesis, the cell cycle, and apoptosis
Authors: He Jun‐Feng , Luo Yu‐Mei , Wan Xin‐Hong , Jiang Deqian 
Published: December 2011

AbstractmicroRNA‐195(miR‐195) is an important member of the micro‐15/16/195/424/497 family, and which is activated in multiple diseases, such as cancers, heart failure, and schizophrenia. Mir‐195 regulates a plethora of target proteins, which are involved in the cell cycle, apoptosis, proliferation. WEE1, CDK6, and Bcl‐2 are confirmed target genes of miR‐195 that are involved in miR‐195‐mediated cell‐cycle and apoptosis effects. However, the mechanism of miR‐195 action is not completely understood. This review summarizes recent the research progress regarding the roles of miR‐195 in the cell cycle and in apoptosis. © 2011 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:404–408 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20396
Proteomic investigation of 5‐fluorouracil resistance in a human hepatocellular carcinoma cell line
Authors: Tong Shi‐Wen , Yang Yi‐Xuan , Hu Huai‐Dong , An Xuan , Ye Feng , Hu Peng , Ren Hong , Li Sang‐Lin , Zhang Da‐Zhi 
Published: December 2011

AbstractMulti‐drug resistance (MDR) is a major obstacle towards a successful treatment of hepatocellular carcinoma (HCC). The mechanisms of MDR are intricate and have not been fully understood. Therefore, we employed a cell‐line model consisting of the 5‐fluorouracil (5‐FU) resistant BEL7402/5‐FU cell line and its parental BEL7402 cell line. Using relative and absolute quantification (iTRAQ)‐coupled 2D LC‐MS/MS, a successfully exploited high‐throughput proteomic technology, in total, 660 unique proteins were identified and 52 proteins showed to be differentially expressed in BEL7402/5‐FU compared with BEL7402. Several differentially expressed proteins were further validated by Western blot and real‐time quantitative RT‐PCR analysis. Furthermore, the association of MDR with ANXA3, one of the highly expressed proteins in BEL7402/5‐FU, was verified. Our study represents the first successful application of iTRAQ technology for MDR mechanisms analysis in HCC. Many of the differentially expressed proteins identified had not been linked to MDR in HCC before, which provide valuable information for further understanding of MDR. J. Cell. Biochem. 113: 1671–1680, 2012. © 2011 Wiley Periodicals, Inc.
Nature of the ring‐closure process along the rearrangement of octa‐1,3,5,7‐tetraene to cycloocta‐1,3,5‐triene from the perspective of the electron localization function and catastrophe theory
Authors: Andrés J. , Berski S. , Domingo L. R. , González‐Navarrete P. 
Published: December 2011

AbstractWe analyze the behavior of the energy profile of the ring‐closure process for the transformation of (3Z,5Z)‐octa‐1,3,5,7‐tetraene5to (1Z,3Z,5Z)‐cycloocta‐1,3,5‐triene6through a combination of electron localization function (ELF) and catastrophe theory (CT). From this analysis, concepts such as bond breaking/forming processes, formation/annihilation of lone pairs, and other electron pair rearrangements arise naturally through the reaction progress simply in terms of the different ways of pairing up the electrons. A relationship between the topology and the nature of the bond breaking/forming processes along this rearrangement is reported. The different domains of structural stability of the ELF occurring along the intrinsic reaction path have been identified. The reaction mechanism consists of six steps separated by fold and cusp catastrophes. The transition structure is observed in the third step,d(C1C8) = 2.342 Å, where all bonds have topological signature of single bonds (CC). The “new” C1C8 single bond is not formed in transition state and respective catastrophe of the ELF field (cusp) is localized in the last step,d(C1C8) ≈ 1.97 Å, where the two monosynaptic nonbonding basins V(C1) and V(C8) are joined into single disynaptic bonding basin V(C1,C8). The V(C1,C8) basin corresponds to classical picture of the C1C8 bond in the Lewis formula. In cycloocta‐1,3,5‐triene6the single C1C8 bond is characterized by relatively small basin population 1.72e, which is much smaller than other single bonds with 2.03 and 2.26e. © 2011 Wiley Periodicals, Inc. J Comput Chem, 2011
Treatment with sodium orthovanadate reduces blood–brain barrier disruption via phosphatase and tensin homolog deleted on chromosome 10 (PTEN) phosphorylation in experimental subarachnoid hemorrhage
Authors: Hasegawa Yu , Suzuki Hidenori , Altay Orhan , Chen Hank , Zhang John H. 
Published: December 2011

AbstractAttenuation of blood–brain barrier (BBB) disruption is one of the therapeutic candidates for treatment of subarachnoid hemorrhage (SAH). In this study, the protective effect of sodium orthovanadate (SOV) on BBB disruption was investigated in SAH using the endovascular perforation model. Fifty‐five rats were randomly assigned to sham‐operated, SAH treated with saline (as a vehicle), or 10 mg/kg SOV groups and were evaluated for neurofunction and Evans blue dye extravasation. The phosphorylation of phosphatase and tensin homolog deleted on chromosome 10 (PTEN) and mitogen‐activated protein kinase (MAPK) and the expression of matrix metalloproteinase‐9 (MMP‐9), occludin, and collagen‐IV were examined by Western blot analyses. Cell death among endothelial cells was revealed by immunofluorescence and terminal deoxynucleotidyl transferase‐mediated uridine 5′‐triphosphate‐biotin nick end‐labeling staining. SOV significantly improved neurofunction and reduced Evans blue dye extravasation in brains after SAH. SOV phosphorylated PTEN, decreased phospho‐JNK and MMP‐9, and preserved occludin expression. SOV also attenuated SAH‐induced capillary endothelial cell death. The current study showed that SOV was protective against BBB disruption after SAH, possibly via PTEN phosphorylation. © 2011 Wiley Periodicals, Inc.
Hypothyroidism reduces glutamate‐synaptic release by ouabain depolarization in rat CA3‐hippocampal region
Authors: Sánchez‐Huerta K.B. , Montes S. , Pérez‐Severiano F. , Alva‐Sánchez C. , Ríos C. , Pacheco‐Rosado J. 
Published: December 2011

AbstractThyroid hormones modulate the physiology of the hippocampus in humans, where glutamate plays an important role as neurotransmitter. The aim of this work was to study the effect of hypothyroidism on hippocampal glutamate extracellular levels, release, uptake, and synthesis. The effects of PDC (a glutamate transporter inhibitor) and ouabain (a Na+/K+‐ATPase inhibitor) infusion on microdialysate glutamate and aspartate levels of CA3 hippocampal region were evaluated. Animals were assigned to one of the following groups: hypothyroid group (Hyp), receiving methimazole (anantithyroid drug); replacement group (Hyp + T4), receiving antithyroid treatment plus thyroxine; and euthyroid control group (Eut). Dialysate fractions were collected every 15 min to determine basal glutamate levels for 1 hr. Then, PDC (10 mM) or ouabain (100 μM) was infused for 30 min. Results showed lower glutamate and aspartate basal levels in Hyp than in Eut groups. PDC infusion increased amino acids levels in all groups, whereas ouabain infusion increased glutamate and aspartate levels only in the Eut group. The infusion of tetrodotoxin (TTX; a voltage‐gated sodium channel inhibitor) prevented the glutamate increase in euthyroid rats. The Hyp + T4 group showed glutamate levels similar to those found in the Eut group. Additionally, glutaminase activity in hippocampus was lower in the Hyp group than in the Eut or Hyp + T4 group. Results suggest that high‐affinity glutamate transporters are not altered by hypothyroidism; however, decreased hypotyroidism reduced vesicular glutamate release in the CA3‐hippocampal region as a consequence of diminished glutamate synthesis. © 2011 Wiley Periodicals, Inc.
Molecular characterization of mulberry (Morus spp.) genotypes via RAPD and ISSR
Authors: İpek Muzaffer , Pirlak Lütfi , Kafkas Salih 
Published: December 2011

AbstractBACKGROUND: In recent years, DNA‐based markers have been used quite extensively because of their many advantages over the traditional morphological and biochemical markers. Many studies have shown that molecular markers are useful in delineating the genetic relationships among closely related mulberry genotypes and cultivars. Thus, in the present study, polymer chain reaction based DNA fingerprinting techniques were used to investigate the genetic relationships among mulberry genotypes growing in different agro‐climatic regions of Turkey.RESULTS: 20 RAPD primers generated a total of 173 bands, of which 157 (90.75%) were polymorphic. As for 11 ISSR primers, 124 bands (96.55%) were polymorphic in a total of 128. The similarity index for RAPD technique ranged between 0.24–0.98; 25İs203 with 25İs112 were found to be the closest genotypes, while 24Ke10 and 25İs123 were the most distant ones. According to the ISSR result, the genetic similarity index changed between 0.21–095; 25İs203 with 25İs112 genotypes were the closest, while 25İs08 and 01KaD2 were the most distant ones.CONCLUSION: The RAPD and ISSR markers were found to be promising for assessing genetic diversity in mulberry genotypes. Copyright © 2011 Society of Chemical Industry
Inhibitory activity of plumbagin produced by Drosera intermedia on food spoilage fungi
Authors: Grevenstuk Tomás , Gonçalves Sandra , Domingos Telma , Quintas Célia , van der Hooft Justin JJ , Vervoort Jacques , Romano Anabela 
Published: December 2011

AbstractBACKGROUND: The aim of this study was to investigate the growth‐inhibiting efficacy of Drosera intermedia extracts (water, methanol and n‐hexane) against four food spoilage yeasts and five filamentous fungi strains responsible for food deterioration and associated with mycotoxin production, in order to identify potential antimycotic agents.RESULTS: The n‐hexane extract showed a broad activity spectrum against all tested microorganisms, followed, in activity, by the methanol and water extracts. The major component of the n‐hexane extract was purified using a solid‐phase extraction column and identified as plumbagin. Results show that high‐purity plumbagin can be produced from D. intermedia cultures following a simple and effective isolation procedure. A sample of purified plumbagin was tested against the same panel of microorganisms and high growth‐inhibiting capacity was observed. Minimum inhibitory concentrations less than 2 µg mL−1 were obtained against the filamentous fungi. In the case of the species Aspergillus fumigatus, A. niger and A. flavus, activities comparable to miconazole were obtained.CONCLUSION: The results obtained provided evidence of the antimycotic activity of plumbagin, suggesting that D. intermedia could be the source of an interesting compound for the food industry as an alternative to preservatives. Copyright © 2011 Society of Chemical Industry
Operative management for recurrent and metastatic adrenocortical carcinoma
Authors: Datrice Nicole M. , Langan Russell C. , Ripley R. Taylor , Kemp Clinton D. , Steinberg Seth M. , Wood Bradford J. , Libutti Steven K. , Fojo Tito , Schrump David S. , Avital Itzhak 
Published: December 2011

AbstractObjectiveA review of all resections for recurrent or metastatic ACC was performed to identify patients who might benefit from a surgical approach, and to identify factors that might aid in prognosis among patients with metastatic disease.Summary Background DataAdrenocortical carcinoma (ACC) is a rare tumor, with frequent recurrences and metastases even after complete resection. Chemotherapy has limited efficacy, and surgical resection of metastatic ACC remains controversial.MethodsA retrospective review was performed of all patients who underwent surgical intervention for metastatic ACC in a single tertiary center from 1977 to 2009. All available clinicopathologic data were analyzed to determine potential factors associated with response to treatment and survival.ResultsFifty‐seven patients underwent 116 procedures for recurrent or metastatic disease. Twenty‐three resections were for liver metastases, 48 for pulmonary metastases, 22 for abdominal disease including local recurrences, and 13 were for metastases at other sites. Median and 5‐year survivals from time of first metastasectomy were 2.5 years, and 41%, respectively. The median survival of patients with DFI <12 months was 1.7 years, compared to 6.6 years for patients with DFI >12 months (P = 0.015). Median survival for right versus left‐sided primaries was 1.9 years versus 3.8 years (P = 0.03). Liver metastases were more common with right‐sided primaries (67% vs. 41%, P = 0.05). Chemotherapy had no impact on survival.ConclusionsResection of recurrent or metastatic ACC is safe, and may result in prolongation of survival in selected patients with DFI greater than 1 year. J. Surg. Oncol. 2012; 105:709–713. © 2011 Wiley Periodicals, Inc.
Calving rate, calf survival rate, and habitat selection of forest‐dwelling caribou in a highly managed landscape
Authors: Pinard Véronique , Dussault Christian , Ouellet Jean‐Pierre , Fortin Daniel , Courtois Réhaume 
Published: December 2011

AbstractLogging negatively affects the threatened forest‐dwelling caribou (Rangifer tarandus caribou) through its positive effects on large predator populations. As recruitment is a key component of caribou population growth rate, we assessed calving rates of females and calf survival rates during the most critical period for calf survival, the calving period. We also identified causes of calf mortality and investigated the influence of predation risk, food availability, and human disturbance on habitat selection of females during the calving period at both the home‐range and forest stand scales. We hypothesized that caribou should display habitat selection patterns to reduce predation risk at both scales. Using telemetry, we followed 22 females and their calves from 2004 to 2007 in a highly managed study area in Québec, Canada. Most females (78.5 ± 0.05 [SE]) gave birth each year, but only 46.3 ± 8.0% of the calves survived during the first 50 days following birth, and 57.3 ± 14.9% of them died from black bear (Ursus americanus) predation. At the home‐range scale, caribou selected calving areas located at upper slope positions and avoided high road density areas. Surprisingly, they also selected the forested habitat type having the lowest lateral cover (mixed and deciduous stands) while avoiding the highest cover (regenerating conifer stands). At the forest stand scale, caribou selected areas located at relatively high elevations and with a lower basal area of black spruce trees. The selection of upper slope positions likely favored spatial segregation between calving females and wolves (Canis lupus) but not black bear. Our results suggest that calving females used areas from which they could visually detect approaching predators. While wolf avoidance appeared to be effective in a highly managed landscape, caribou did not appear to have adjusted their predator avoidance strategy to the recent increase in black bear abundance, who have benefited from increased food abundance. This situation requires focused attention from wildlife managers as logging activities are progressing towards the north within the core of forest‐dwelling caribou range. © 2011 The Wildlife Society.
Learning From Nature: Synthesis and Characterization of Longitudinal Polymer Gradient Materials Inspired by Mussel Byssus Threads
Authors: Claussen Kai U. , Giesa Reiner , Scheibel Thomas , Schmidt Hans‐Werner 
Published: December 2011

AbstractMarine mussels use their threads for attachment to any substratum and these biopolymer gradient fibers show an excellent combination of stiff and soft mechanical properties. A straightforward approach for the preparation of macroscopic longitudinal polymer gradient materials on the centimeter scale based on a poly(dimethyl siloxane) system is presented. Compositional gradients are realized by using three syringe pumps feeding different prepolymers capable to undergo thermal cross‐linking. Within the gradient samples, the stiffness between the hard and soft part can be varied up to a factor of four. The gradients are analyzed by UV–Vis spectroscopy as well as compressive and tensile modulus testing.
A Facile Route to Reassemble Titania Nanoparticles Into Ordered Chain‐like Networks on Substrate
Authors: Cheng Ya‐Jun , Wolkenhauer Markus , Bumbu Gina‐Gabriela , Gutmann Jochen S. 
Published: December 2011

AbstractA facile route to reassemble titania nanoparticles within the titania‐block copolymer composite films has been developed. The titania nanoparticles templated by the amphiphilic block copolymer of poly(styrene)‐block‐poly (ethylene oxide) (PS‐b‐PEO) were frozen in the continuous PS matrix. Upon UV exposure, the PS matrix was partially degraded, allowing the titania nanoparticles to rearrange into chain‐like networks exhibiting a closer packing. The local structures of the Titania chain‐like networks were investigated by both AFM and SEM; the lateral structures and vertical structures of the films were studied by GISAXS and X‐ray reflectivity respectively. Both the image analysis and X‐ray scattering characterization prove the reassembly of the titania nanoparticles after UV exposure. The mechanism of the nanoparticle assembly is discussed.
Functionalized Nanoporous Thin Films From Photocleavable Block Copolymers
Authors: Schumers Jean‐Marc , Vlad Alexandru , Huynen Isabelle , Gohy Jean‐François , Fustin Charles‐Andre 
Published: December 2011

AbstractA polystyrene‐block‐poly(ethylene oxide) block copolymer bearing a photocleavable junction between the blocks is used to form nanoporous thin films with carboxylic acid functions homogeneously distributed on the pore walls. The presence of the carboxylic acid groups is evidenced by fluorescence spectroscopy after their reaction with a diazomethane functionalized fluorescent dye. In addition, the initial light‐responsive thin film, acting as a photoresist, can be easily patterned to selectively generate porosity in predetermined areas. In that way, fluorescent patterns can be obtained as evidenced by fluorescent microscopy.
On the role of particle breakage in the shear failure behavior of granular soils by DEM
Authors: Wang Jianfeng , Yan Haibin 
Published: December 2011

SUMMARYThis article presents a fundamental study on the role of particle breakage on the shear behavior of granular soils using the three‐dimensional (3‐D) discrete element method. The effects of particle breakage on the stress ratio, volumetric strain, plastic deformation, and shear failure behavior of dense crushable specimens undergoing plane strain shearing conditions are thoroughly investigated through a variety of micromechanical analyses and mechanism demonstrations. The simulation of a granular specimen is based on the effective modeling of realistic fracture behavior of single soil particles, which is demonstrated by the qualitative agreement between the results from platen compression simulations and those from physical laboratory tests.The simulation results show that the major effects of particle breakage include the reduction of volumetric dilation and peak stress ratio and more importantly the plastic deformation mechanisms and the shear failure modes vary as a function of soil crushability. Consistent macro‐ and micromechanical evidence demonstrates that shear banding and massive volumetric contraction depict the two end failure modes of a dense specimen, which is dominated by particle rearrangement–induced dilation and particle crushing–induced compression, respectively, with a more general case being the combination and competition of the two failure modes in the medium range of soil crushability and confining stress. However, it is further shown that a highly crushable specimen will eventually develop a shear band at a large strain because of the continuous decay of particle breakage. Copyright © 2011 John Wiley & Sons, Ltd.
Continuous non‐invasive measurement of bladder voiding pressure using an experimental constant low‐flow test
Authors: Clarkson Becky , Griffiths Clive , McArdle Frank , Pickard Robert , Drinnan Michael 
Published: December 2011

AbstractPurposeWe developed a prototype device using an automatically controlled inflatable penile cuff to measure bladder voiding pressure continuously and non‐invasively in men. The purpose of this study was to validate the pressure measurements made by this device by comparison to those during simultaneous invasive PFS.MethodsA device was developed to automatically modulate flow rate by controlling pressure in a penile cuff. Men undergoing conventional urodynamics studies were recruited to have an additional fill–void cycle whilst using the new device. We report on 40 men using a standardized protocol. Pressure measured by this device was compared with simultaneous vesical pressure by evaluating maximum pressure during voiding, and calculating the root mean square (RMS) difference between pcuff and pves.ResultsWe recruited 80 men of whom 18 were excluded, 22 were involved in initial development phase and 40 in the standard protocol. Mean (SD) difference between maxima of pcuff and pves (1.3 (15.6) cmH2O) was not significant. In 20 (50%) men, agreement between pcuff and pves throughout the void was moderate or good (RMS difference <20 cmH2O). Causes of unsuccessful measurements were identified.ConclusionsAlthough a prototype device, this technique appears to be promising for continuous non‐invasive bladder pressure measurement. From the results of this study a number of improvements have been recommended and implemented. The next generation of this technique will allow assessment of clinical application. Neurourol. Urodynam. 31:557–563, 2012. © 2011 Wiley Periodicals, Inc.
Self‐reported Knowledge, Attitudes and Behaviour towards Evidence‐based Practice of Occupational Therapy Students in Ireland
Authors: Stronge M , Cahill M 
Published: December 2011

AbstractIt is widely accepted that the occupational therapy profession needs to incorporate research findings into clinical practice so as to improve client outcomes. The purpose of this study was to investigate the knowledge and attitudes toward evidence‐based practice (EBP) of occupational therapy students in the Republic of Ireland. A validated questionnaire was used to survey the population of final‐year students from the four universities in Ireland in 2008. There was a response rate of 77% (n = 86) to the Knowledge, Attitude and Behaviour Questionnaire. All students reported that they had a clear understanding of EBP and were willing to practice EBP in the future. The majority (85%, n = 73) reported accessing evidence weekly or more often. Lack of time and fieldwork educators not practising EBP were important barriers for 31% (n = 27) and 27% (n = 23), respectively. Over half (55%, n = 47) reported difficulty in finding evidence. The internet (28%, n = 24) and textbooks (27%, n = 23) were the most popular sources of evidence. Limitations include the self‐report, cross‐sectional design. Future research could include longitudinal studies to understand the transfer of learning into clinical practice. Copyright © 2011 John Wiley & Sons, Ltd.
Novel and recurrent mutations of ITGA2B and ITGB3 genes in Korean patients with Glanzmann thrombasthenia
Authors: Park Kyoung‐Jin , Chung Hae‐Sun , Lee Ki‐O , Park In‐Ae , Kim Sun‐Hee , Kim Hee‐Jin 
Published: December 2011

AbstractGlanzmann thrombasthenia (GT) is an autosomal recessive bleeding disorder caused by defective glycoprotein, αIIb and β3, encoded by ITGA2B and ITGB3 genes, respectively. We herein describe four unrelated Korean patients with genetically confirmed GT. Two patients were homozygous for c.1913+5G>T (IVS11+5G>T) mutation of ITGB3 with a signature of founder effect. The other two patients were compound heterozygous for two mutations of ITGA2B: c.[2333A>C];[2975delA] (p.[Q778P];[E992Gfs*30]) and c.[1750C>T];[2333A>C] (p.[R584X];[Q778P]). The c.2975delA mutation was a novel frameshift mutation of ITGA2B. Although from a limited number of patients, these results suggests c.1913+5G>T of ITGB3 is a recurrent mutation in Korean patients with GT. Pediatr Blood Cancer 2012;59:335–338. © 2011 Wiley Periodicals, Inc.