bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimization and Characterization of Cementitious Composites Combining Maximum Amounts of Waste Glass Powder and Treated Glass Aggregates
Authors: Mezaouri Sarra , Kameche Zine El-Abidine , Siad Hocine , Lachemi Mohamed , Sahmaran Mustafa , Houmadi Youcef 
Published: May 2024

AbstractThis work investigates the combined use of waste glass aggregates (GA) and glass powder (GP) in cementitious mortars. For this reason, the optimized incorporation of GA by natural aggregates (NA) replacements was first studied after applying a surface roughening method with hydrofluoric acid. The compressive strength results were utilized to select the best mixture with GA. Then, different GP contents were added by cements substitutions to the optimized GA-based mortar. A control mortar without GA and GP amounts was also casted as a reference for comparison. The detailed mechanical, physical and durability properties of the resulted mixtures with combined GA and GP were assessed by considering the compressive and flexural strengths, ultra-sonic pulse velocity, alkali-silica reaction (ASR), rapid chloride permeability test (RCPT), magnesium sulphate attack and sulfuric acid resistance. The microstructure of different optimized (GA + GP)-combinations was characterized by scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDS)in order to analyse the interfacial transition zone (ITZ) between glass materials and the surrounding matrix. The results showed that the optimized composition with 75% GA and 25% GP was shown with high compacity and durability characteristics due to the increased GA/matrix ITZ and the formation of C–(N,K)–S–H products with C–S–H.
A survey of ictal physical examination during VEEG monitoring in a tertiary epilepsy center
Authors: Li Yinping , Hu Xiaoying , Zhang Shufang , Chen Jiani 
Published: May 2024

Abstract
Background
Ictal examination based on video-based electroencephalography (EEG) is crucial for locating and lateralizing seizures. In this study, we aimed to evaluate the quality of ictal examination in the Comprehensive Epilepsy Center of West China Hospital, Sichuan University, in order to provide information for quality improvement in daily clinical practice.

Methods
Video recordings of 100 patients with epilepsy were retrospectively reviewed. The performance of the ictal examination was independently reviewed by two epileptologists using an ictal examination protocol.

Results
In this retrospective analysis, 589 seizure episodes from 100 patients with epilepsy were reviewed. The ages of the patients ranged from 3 to 77 years, with a mean age of 25.8 ± 12.8 years. Among the 589 seizure episodes, a majority (93.7%) were focal seizures. For 226 (38.4%) seizures, the medical staff arrived at the bedside. Among them, 153 (153/226, 64.7%) seizure episodes, the medical staff arrival at the bedside within 30 s of onset, and 120 (120/226, 53.1%) seizures were tested by the medical staff. The compliance rates for "safety” and "visibility" reached 80% or higher while “naming”, “retelling”, and “memory testing” only reach less than 3%.

Conclusions
Our survey identified the main problems in ictal assessments. It is challenging to complete a standardized examination for new trainees at Epilepsy Monitoring Units. Regularly strengthening training in ictal examination and understanding of semiology may improve patients’ examination ability. However, further study of the implementation of training is necessary.
Therapeutic potential of gelatine methacrylate hydrogels loaded with macrophage-derived exosomes for accelerating angiogenesis and cutaneous wound healing
Authors: Liu Jiajun , Chen Fuying , Tian Luoqiang , Wu Jinjie , Liu Keting , Wan Qiwen , Yuan Bo , Zhu Xiangdong , Chen Xuening , Zhang Xingdong 
Published: May 2024

AbstractExtensive studies demonstrate that macrophage response plays an important role in regulating angiogenesis via a paracrine way, which is crucial for skin wound repair. This study isolated and characterized nanosized exosomes from differently polarized macrophages (MΦ), including M0 (naïve), M1 (pro-inflammatory), and M2 (anti-inflammatory) macrophages, and further assessed their impacts on angiogenesis and skin regeneration. Our results indicated that compared to M0 and M1 counterparts, M2 macrophage-derived exosomes (M2-Exos) exhibited a pronounced ability to promote angiogenic ability of of human umbilical vein endothelial cells (HUVECs) by enhancing expression of angiogenic genes and proteins, increasing cell migration, and improving tubulogenesis. Bioinformatics analyses suggested that the distinct angiogenic potentials of three MΦ-Exos might be attributed to the differentially expressed angiogenesis-related miRNAs and their target genes such as Stat3, Smad 2, and Smad4. Moreover, these isolated MΦ-Exos were integrated with gelatine methacrylate (GelMA) hydrogels to achieve the sustained delivery at murine full-thickness cutaneous wound sites. In vivo results showed that Gel/M2-Exos significantly augmented angiogenesis, accelerated re-epithelialization, promoted collagen maturity, thereby promoting wound healing. In contrary, Gel/M1-Exos showed the opposite effects. Our findings provided compelling evidence that the polarization status of macrophages significantly affected angiogenesis and wound healing via the miRNA cargos of their derived exosomes. Moreover, this study opens a new avenue for developing nano-scale, cell-free exosome-based therapies in treating cutaneous wounds.
Graphical abstract
Prevalence of GIT symptoms in patients of COVID 19 and role of rectal PCR in detecting COVID 19 with GIT symptoms
Authors: Ahmed Ahmed Elmetwally , Awad Emad Ahmed , Mohamed Manal Sabry , Abdelrady Abdelrhman Haggag , Ismail Moamen Abdelfadil , El-Gaaly Sonya Ahmed Ali 
Published: May 2024

Abstract
Background
In December 2019, a cluster of patients with pneumonia of undetermined etiology was recognized in Wuhan, Hubei, China. Subsequently, a novel coronavirus (Severe Acute Respiratory Distress Syndrome- related Coronavirus) (SARS-CoV-2) was identified from lower respiratory tract samples obtained from affected patients. The clinical manifestation of Coronavirus disease 2019 (COVID 19) is broad and ranges from asymptomatic and mild upper respiratory tract symptoms to severe illnesses with multi-organ failure and death. Furthermore, it is challenging to predict the clinical course or determine patients at risk of deterioration.

Aim of the work
The aim of our study is to assess prevalence of gastrointestinal tract (GIT) symptoms in COVID 19 infected patients and to assess significance of rectal PCR in detecting COVID 19 patients with gastrointestinal symptoms.

Patients and methods
This study was conducted on 100 adult COVID 19 patients recently diagnosed by polymerase chain reaction (PCR). All patients were submitted to clinical examination, laboratory testing for Erythrocyte Sedimentation Rate (ESR), C-Reactive Protein,(CRP), Complete Blood Count (CBC), and D-dimer. Radiological investigations in the form of Computed Tomography Chest were reported by radiologist for all patients (High resolution computed tomography). Nasopharyngeal, oropharyngeal and rectal swabs were collected for COVID-19 (PCR) test. All patients received COVID-19 treatment according to protocols of World Health Organization (WHO) and Ministry of Health and Population, Egypt.

Results
We found that GI symptoms are prevalent among COVID-19 Egyptian patients (64%). The most common GIT symptoms were Nausea, vomiting and diarrhea. We observed that 25 patients (25%) had positive viral RiboNucleic Acid (RNA) in rectal swab. Nausea was manifested in 38 patients (38%), Vomiting was manifested in 24 patients (24%), diarrhea was manifested in 21 patients (21%), pain was manifested in 22 patients (22%), hematemesis was manifested in 3 patients (3%) and melena was manifested in 2 patients (2%).

Conclusion
The results of current study demonstrated that GIT symptoms are prevalent among COVID-19 Egyptian patients (64%) with Nausea, vomiting and diarrhea to be most common symptoms. Rectal PCR was found in 25 patients, all of them had GIT symptoms but it was statistically non-significant result when compared to the overall number of COVID-19 infected patients. Severe COVID-19 was more frequent in older age.
Elective Pelvic Lymph Node Radiation Therapy and the Risk of Death in Patients With Unfavorable-Risk Prostate Cancer: A Postrandomization Analysis
Authors: Sayan Mutlay , Chen Ming-Hui , Loffredo Marian , McMahon Elizabeth , Moningi Shalini , Orio Peter F. , Nguyen Paul L. , D'Amico Anthony V. 
Published: May 2024

PURPOSE Although a contemporary randomized clinical trial has led to the use of whole-pelvic radiation therapy (WPRT), long-term data evaluating a potential reduction in mortality are lacking and are addressed in the current study. MATERIALS AND METHODS From 2005 to 2015, 350 men with localized, unfavorable-risk prostate cancer (PC) were randomly assigned to receive androgen deprivation therapy (ADT) and RT plus docetaxel versus ADT and RT. Treatment of the pelvic lymph nodes was at the discretion of the treating physician. Multivariable Cox and Fine and Grays regression analyses were performed to assess whether a significant association existed between radiation treatment volume and all-cause mortality (ACM) and PC-specific mortality (PCSM), respectively, adjusting for known PC prognostic factors and comorbidity. An interaction term between age (categorized by dichotomization at 65 years to enable clinical interpretation and applicability of the results and which approximates the median (66 years [IQR, 61-70]) and radiation treatment volume was included in the analysis. RESULTS After a median follow-up of 10.20 years (IQR, 7.96-11.41), 89 men died (25.43%); of these, 42 died of PC (47.19%). Of the 350 randomly assigned patients, 88 (25.14%) received WPRT. In men younger than 65 years, WPRT was associated with a significantly lower ACM risk (adjusted hazard ratio [AHR], 0.33 [95% CI, 0.11 to 0.97]; P = .04) and lower PCSM risk (AHR, 0.17 [95% CI, 0.02 to 1.35]; P = .09) after adjusting for covariates, whereas this was not the case for men 65 years or older. CONCLUSION WPRT has the potential to reduce mortality in younger men with unfavorable-risk PC.
Natural History and Genomic Landscape of Chemotherapy-Resistant Muscle-Invasive Bladder Cancer
Authors: Lenis Andrew T. , Whiting Karissa , Ravichandran Vignesh , Tallman Jacob E. , Alam Syed M. , Chu Carissa E. , Jesus Escano Manual De , Bochner Emily , Katims Andrew , Reisz Peter A. , Truong Hong , Clinton Timothy N. , Telis Leon , Dason Shawn , McPherson Victor , Teo Min Yuen , Funt Samuel , Aggen David , Goh Alvin C. , Donahue Timothy F. , Cha Eugene K. , Donat S. Machele , Herr Harry W. , Dalbagni Guido , Schultz Nikolaus , Berger Michael F. , Bajorin Dean F. , Rosenberg Jonathan E. , Bochner Bernard H. , Ostrovnaya Irina , Al-Ahmadie Hikmat , Solit David B. , Iyer Gopa , Pietzak Eugene J. 
Published: May 2024

PURPOSE Patients with residual invasive bladder cancer after neoadjuvant chemotherapy (NAC) and radical cystectomy have a poor prognosis. Data on adjuvant therapy for these patients are conflicting. We sought to evaluate the natural history and genomic landscape of chemotherapy-resistant bladder cancer to inform patient management and clinical trials. METHODS Data were collected on patients with clinically localized muscle-invasive urothelial bladder cancer treated with NAC and cystectomy at our institution between May 15, 2001, and August 15, 2019, and completed four cycles of gemcitabine and cisplatin NAC, excluding those treated with adjuvant therapies. Survival was estimated using the Kaplan-Meier method, and multivariable Cox proportional hazards models were used to identify predictors of recurrence-free survival (RFS). Genomic alterations were identified in targeted exome sequencing (Memorial Sloan Kettering Integrated Mutation Profiling of Actionable Cancer Targets) data from post-NAC specimens from a subset of patients. RESULTS Lymphovascular invasion (LVI) was the strongest predictor of RFS (hazard ratio, 2.15 [95% CI, 1.37 to 3.39]) on multivariable analysis. Patients with ypT2N0 disease without LVI had a significantly prolonged RFS compared with those with LVI (70% RFS at 5 years). Lymph node yield did not affect RFS. Among patients with sequencing data (n = 101), chemotherapy-resistant tumors had fewer alterations in DNA damage response genes compared with tumors from a publicly available chemotherapy-naïve cohort (15% v 29%; P = .021). Alterations in CDKN2A/B were associated with shorter RFS. PIK3CA alterations were associated with LVI. Potentially actionable alterations were identified in more than 75% of tumors. CONCLUSION Although chemotherapy-resistant bladder cancer generally portends a poor prognosis, patients with organ-confined disease without LVI may be candidates for close observation without adjuvant therapy. The genomic landscape of chemotherapy-resistant tumors is similar to chemotherapy-naïve tumors. Therapeutic opportunities exist for targeted therapies as adjuvant treatment in chemotherapy-resistant disease.
Serial Postoperative Circulating Tumor DNA Assessment Has Strong Prognostic Value During Long-Term Follow-Up in Patients With Breast Cancer
Authors: Shaw Jacqueline A. , Page Karen , Wren Evie , de Bruin Elza C. , Kalashnikova Ekaterina , Hastings Robert , McEwen Rob , Zhang Eddie , Wadsley Marc , Acheampong Emmanuel , Renner Derrick , Gleason Kelly L.T. , Ambasager Bana , Stetson Daniel , Fernandez-Garcia Daniel , Guttery David , Allsopp Rebecca C. , Rodriguez Angel , Zimmermann Bernhard , Sethi Himanshu , Aleshin Alexey , Liu Minetta C. , Richards Cathy , Stebbing Justin , Ali Simak , Rehman Farah , Cleator Susan , Kenny Laura , Ahmed Samreen , Armstrong Anne C. , Coombes R. Charles 
Published: May 2024

PURPOSE
Here, we report the sensitivity of a personalized, tumor-informed circulating tumor DNA (ctDNA) assay (Signatera) for detection of molecular relapse during long-term follow-up of patients with breast cancer.

METHODS
A total of 156 patients with primary breast cancer were monitored clinically for up to 12 years after surgery and adjuvant chemotherapy. Semiannual blood samples were prospectively collected, and analyzed retrospectively to detect residual disease by ultradeep sequencing using ctDNA assays, developed from primary tumor whole-exome sequencing data.

RESULTS

Personalized Signatera assays detected ctDNA ahead of clinical or radiologic relapse in 30 of the 34 patients who relapsed (patient-level sensitivity of 88.2%). Relapse was predicted with a lead interval of up to 38 months (median, 10.5 months; range, 0-38 months), and ctDNA positivity was associated with shorter relapse-free survival (
P
< .0001) and overall survival (
P
< .0001). All relapsing triple-negative patients (n = 7/23) had a ctDNA-positive test within a median of 8 months (range, 0-19 months), while the 16 nonrelapsed patients with triple-negative breast cancer remained ctDNA-negative during a median follow-up of 58 months (range, 8-99 months). The four patients who had negative tests before relapse all had hormone receptor–positive (HR+) disease and conversely, five of the 122 nonrelapsed patients (all HR+) had an occasional positive test.

CONCLUSION
Serial postoperative ctDNA assessment has strong prognostic value, provides a potential window for earlier therapeutic intervention, and may enable more effective monitoring than current clinical tests such as cancer antigen 15-3. Our study provides evidence that those with serially negative ctDNA tests have superior clinical outcomes, providing reassurance to patients with breast cancer. For select cases with HR+ disease, decisions about treatment management might require serial monitoring despite the ctDNA-positive result.
Quantum thermometry based on interferometric power
Authors: Yang Hongying , Zheng Qiang , Yue Ping , Zhi Qijun 
Published: May 2024

Abstract
In the field of quantum thermometry, usually temperature is estimated by the framework of quantum metrology. In this work, an alternative approach to quantum thermometry is suggested, based on interferometric power (IP). IP is defined as the worst-case quantum Fisher information in a double-channel interferometer. Specifically, the time evolution of the IP for a two-qubit state as a probe contacting with a finite-temperature bath is considered. The IP dynamics of the probe with three kinds of initial states (i.e., entangled, separable, and mixed) strongly depend on the bath temperature. The dynamical evolution of IP would be measured experimentally, considering that the IP is a measurable quantity in the experiment. Thus, the IP dynamics can be adopted to extract the value of the bath temperature directly. In this sense, the IP could be exploited as a quantum thermometer.
Subtemporal Approach for the Treatment of Ruptured and Unruptured Distal Basilar Artery Aneurysms: Is There a Contemporary Use?
Authors: Findlay Matthew C. , Bounajem Michael T. , Kim Robert B. , Henson J. Curran , Azab Mohammed A. , Cutler Christopher B. , Khan Majid , Brandon Cameron , Budohoski Karol P. , Rennert Robert C. , Couldwell William T. 
Published: May 2024

BACKGROUND AND OBJECTIVES:
Distal basilar artery aneurysms (DBAs) are high-risk lesions for which endovascular treatment is preferred because of their deep location, yet indications for open clipping nonetheless remain. The subtemporal approach allows for early proximal control and direct visualization of critical posterior perforating arteries, especially for posterior-projecting aneurysms. Our objective was to describe our clinical experience with the subtemporal approach for clipping DBAs in the evolving endovascular era.

METHODS:
This was a retrospective, single-institution case series of patients with DBAs treated with microsurgery over a 21-year period (2002-2023). Demographic, clinical, and surgical data were collected for analysis.

RESULTS:
Twenty-seven patients underwent clipping of 11 ruptured and 16 unruptured DBAs with a subtemporal approach (24 female; mean age 53 years). Ten patients had expanded craniotomies for treatment of additional aneurysms. The aneurysm occlusion rate was 100%. Good neurological outcomes as defined by the modified Rankin Scale score ≤2 and Glasgow Outcome Scale score ≥4 were achieved in 21/27 patients (78%). Two patients died before hospital discharge, one from vasospasm-induced strokes and another from an intraoperative myocardial infarction.

CONCLUSION:
These results demonstrate that microsurgical clip ligation of DBAs using the subtemporal approach remains a viable option for complex lesions not amenable to endovascular management.
HIF-1α-dependent upregulation of angiogenic factors by mechanical stimulation in retinal pigment epithelial cells
Authors: Ashimori Atsushige , Higashijima Fumiaki , Ogata Tadahiko , Sakuma Ayano , Hamada Waka , Sunada Junki , Aoki Ren , Mikuni Masanori , Hayashi Ken'ichiro , Wakuta Makiko , Yoshimoto Takuya , Minamoto Akira , Ko Ji-Ae , Kimura Kazuhiro 
Published: May 2024

ABSTRACT
Mechanical stimulation as a mimic of drusen formation in the eye increases the expression of angiogenic factors in retinal pigment epithelial (RPE) cells, but the underlying molecular mechanisms remain unclear. We investigated and characterized the effects of mechanical stimulation on the expression of angiogenic factors in RPE cells both in vitro and in a mouse model. Mechanical stimulation increased the expression of vascular endothelial growth factor (VEGF, encoded by VEGFA) and other angiogenesis-related genes in cultured RPE1 cells. The presence of hypoxia-inducible factor 1α (HIF-1α, encoded by HIF1A) was also increased, and both knockdown of HIF-1α and treatment with the HIF-1α inhibitor CAY10585 attenuated the effect of mechanical stimulation on angiogenesis factor gene expression. Signaling by the tyrosine kinase SRC and p38 mitogen-activated protein kinase was involved in HIF-1α activation and consequent angiogenesis-related gene expression induced by mechanical stimulation. Our results suggest that SRC–p38 and HIF-1α signaling are involved in the upregulation of angiogenic factors in RPE cells by mechanical stimulation. Such in vivo suppression of upregulated expression of angiogenesis-related genes by pharmacological inhibitors of HIF-1α suggests a new potential approach to the treatment of age-related macular degeneration.
pHusion – a robust and versatile toolset for automated detection and analysis of exocytosis
Authors: O'Shaughnessy Ellen C. , Lam Mable , Ryken Samantha E. , Wiesner Theresa , Lukasik Kimberly , Zuchero J. Bradley , Leterrier Christophe , Adalsteinsson David , Gupton Stephanie L. 
Published: May 2024

ABSTRACT
Exocytosis is a fundamental process used by eukaryotes to regulate the composition of the plasma membrane and facilitate cell–cell communication. To investigate exocytosis in neuronal morphogenesis, previously we developed computational tools with a graphical user interface to enable the automatic detection and analysis of exocytic events from fluorescence timelapse images. Although these tools were useful, we found the code was brittle and not easily adapted to different experimental conditions. Here, we developed and validated a robust and versatile toolkit, named pHusion, for the analysis of exocytosis, written in ImageTank, a graphical programming language that combines image visualization and numerical methods. We tested pHusion using a variety of imaging modalities and pH-sensitive fluorophores, diverse cell types and various exocytic markers, to generate a flexible and intuitive package. Using this system, we show that VAMP3-mediated exocytosis occurs 30-times more frequently in melanoma cells compared with primary oligodendrocytes, that VAMP2-mediated fusion events in mature rat hippocampal neurons are longer lasting than those in immature murine cortical neurons, and that exocytic events are clustered in space yet random in time in developing cortical neurons.
Automated escape system: identifying prey's kinematic and behavioral features critical for predator evasion
Authors: Sunami Nozomi , Kimura Hibiki , Ito Hidechika , Hashimoto Koichi , Sato Yuta , Tachibana Soki , Hidaka Mikiya , Miyama Kouki , Watanabe Hirofumi , Kawabata Yuuki 
Published: May 2024

ABSTRACT
Identifying the kinematic and behavioral variables of prey that influence evasion from predator attacks remains challenging. To address this challenge, we have developed an automated escape system that responds quickly to an approaching predator and pulls the prey away from the predator rapidly, similar to real prey. Reaction distance, response latency, escape speed and other variables can be adjusted in the system. By repeatedly measuring the response latency and escape speed of the system, we demonstrated the system's ability to exhibit fast and rapid responses while maintaining consistency across successive trials. Using the live predatory fish species Coreoperca kawamebari, we show that escape speed and reaction distance significantly affect the outcome of predator–prey interactions. These findings indicate that the developed escape system is useful for identifying kinematic and behavioral features of prey that are critical for predator evasion, as well as for measuring the performance of predators.