bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hypocretin-1 receptor antagonism improves inhibitory control during the Go/No-Go task in highly motivated, impulsive male mice
Authors: Metha Jeremy , Ji Yijun , Braun Clemens , Nicholson Janet R. , De Lecea Luis , Murawski Carsten , Hoyer Daniel , Jacobson Laura H. 
Published: June 2024

Abstract
Rationale
Motivation and inhibitory control are dominantly regulated by the dopaminergic (DA) and noradrenergic (NA) systems, respectively. Hypothalamic hypocretin (orexin) neurons provide afferent inputs to DA and NA nuclei and hypocretin-1 receptors (HcrtR1) are implicated in reward and addiction. However, the role of the HcrtR1 in inhibitory control is not well understood.

Objectives
To determine the effects of HcrtR1 antagonism and motivational state in inhibitory control using the go/no-go task in mice.

Methods
n = 23 male C57Bl/6JArc mice were trained in a go/no-go task. Decision tree dendrogram analysis of training data identified more and less impulsive clusters of animals. A HcrtR1 antagonist (BI001, 12.5 mg/kg, per os) or vehicle were then administered 30 min before go/no-go testing, once daily for 5 days, under high (food-restricted) and low (free-feeding) motivational states in a latin-square crossover design. Compound exposure levels were assessed in a satellite group of animals.

Results
HcrtR1 antagonism increased go accuracy and decreased no-go accuracy in free-feeding animals overall, whereas it decreased go accuracy and increased no-go accuracy only in more impulsive, food restricted mice. HcrtR1 antagonism also showed differential effects in premature responding, which was increased in response to the antagonist in free-feeding, less impulsive animals, and decreased in food restricted, more impulsive animals. HcrtR1 receptor occupancy by BI001 was estimated at ~ 66% during the task.

Conclusions
These data indicate that hypocretin signalling plays roles in goal-directed behaviour and inhibitory control in a motivational state-dependant manner. While likely not useful in all settings, HcrtR1 antagonism may be beneficial in improving inhibitory control in impulsive subpopulations.
2G-lactic acid from olive oil supply chain waste: olive leaves upcycling via Lactobacillus casei fermentation
Authors: Gugel Irene , Marchetti Filippo , Costa Stefania , Gugel Ilenia , Baldini Erika , Vertuani Silvia , Manfredini Stefano 
Published: June 2024

AbstractThe transition towards a sustainable model, particularly the circular economy, emphasizes the importance of redefining waste as a valuable resource, paving the way for innovative upcycling strategies. The olive oil industry, with its significant output of agricultural waste, offers a promising avenue for high-value biomass conversion into useful products through microbial processes. This study focuses on exploring new, high-value applications for olive leaves waste, utilizing a biotechnological approach withLactobacillus caseifor the production of second-generation lactic acid. Contrary to initial expectations, the inherent high polyphenol content and low fermentable glucose levels in olive leaves posed challenges for fermentation. Addressing this, an enzymatic hydrolysis step, following a preliminary extraction process, was implemented to increase glucose availability. Subsequent small-scale fermentation tests were conducted with and without nutrient supplements, identifying the medium that yielded the highest lactic acid production for scale-up. The scaled-up batch fermentation process achieved an enhanced conversion rate (83.58%) and specific productivity (0.26 g/L·h). This research confirms the feasibility of repurposing olive waste leaves for the production of lactic acid, contributing to the advancement of a greener economy through the valorization of agricultural waste.Key points• Olive leaves slurry as it did not allow L. casei to ferment.• High concentrations of polyphenols inhibit fermentation of L. casei.• Enzymatic hydrolysis combined to organosolv extraction is the best pretreatment for lactic acid production starting from leaves and olive pruning waste.Graphical Abstract
Coccygectomy for refractory coccydynia, old-fashioned but effective procedure: A retrospective analysis
Authors: Perna Andrea , Franchini Andrea , Macchiarola Luca , Maruccia Francesco , Barletta Felice , Bosco Francesco , Rovere Giuseppe , Gorgoglione Franco Lucio 
Published: June 2024

Abstract
Purpose
Coccydynia, characterized by persistent pain in the coccygeal region, significantly impacts patients' quality of life. While various treatment modalities exist, including conservative measures and surgical interventions like coccygectomy, optimal management remains unclear. This retrospective cohort study aimed to compare the clinical outcomes, functional improvements, and quality of life in patients with chronic coccydynia undergoing either infiltrative treatment or coccygectomy.

Methods
Data from patients treated at our institution from January 2018 to December 2022 were analyzed. Participants meeting inclusion criteria were divided into two groups: Group A underwent coccygectomy, while Group B received conservative therapy. Clinical assessments, radiographic evaluations, and patient-reported outcomes were collected preoperatively and at follow-up intervals.

Results
Of the 223 initially examined patients, 55 met inclusion criteria. Group A (n = 21) underwent coccygectomy, while Group B (n = 34) received conservative therapy. Both groups showed significant pain reduction post-intervention, with sustained improvement in Group A. Functional outcomes favoured Group A, with significant improvements in disability and quality of life measures. Complications were minimal, with only one case of superficial wound infection in Group A.

Conclusion
Our findings suggest that coccygectomy provides superior and lasting pain relief, functional improvement, and quality of life improvement compared to conservative therapy. While complications were minimal, further research with larger cohorts is warranted to validate these results and explore long-term outcomes. Despite its historical association with complications, advancements in surgical techniques and perioperative care have led to improved outcomes and reduced complication rates. Thus, coccygectomy should be considered in the treatment algorithm for patients with debilitating coccydynia.
Prolonged indwelling catheter time after RARP does not lead to follow-up surgery
Authors: Lenart Sebastian , Holub Markus , Gutjahr Georg , Berger Ingrid , Ponholzer Anton 
Published: June 2024

Abstract
Background
Indwelling catheterization following radical prostatectomy is used to aid healing and urinary drainage. While early removal is well investigated, prolonged catheterization has only been investigated in terms of urinary incontinence. Other complications such as anastomotic strictures are unexplored so far. This study aims to analyze the sequelae of catheterization lasting more than 14 days after robotic-assisted radical prostatectomy (RARP).

Methods
A prospective database of 3087 patients undergoing RARP was analyzed, focusing on 180 patients with catheterization exceeding 14 days (Group A) and 88 matched controls (Group B). Outcome measures included subsequent surgeries, complications, and functional outcomes.

Results
Prolonged catheterization did not significantly increase the need for subsequent surgeries (6% in Group A vs. 7% in Group B, p = .95). However, anastomotic strictures were more common in Group A (3%) compared to Group B (0%) after exclusion of risk factors. Incontinence rates were similar between groups, although a subgroup analysis revealed higher incontinence rates in patients with catheterization exceeding 28 days. No significant differences were observed in erectile function or quality of life between the groups.

Conclusion
Prolonged catheterization after RARP does not independently increase the risk of anastomotic strictures in the general population. However, in patients without risk factors, prolonged catheter dwell time may elevate the risk of strictures and subsequent surgeries. Additionally, patients with catheterization exceeding 28 days may experience higher rates of long-term incontinence. Further studies with larger sample sizes are needed to confirm these findings and elucidate the long-term implications of prolonged catheterization.
Patient experience and satisfaction after same-day discharge radical prostatectomy using a personalized, digital perioperative programme
Authors: d’Agate Daniele , Martini Alberto , Lesourd Marine , Tollon Christophe , Loison Guillaume , Almeras Christophe , Pradère Benjamin , Salin Ambroise , Beauval Jean-Baptiste , Ploussard Guillaume 
Published: June 2024

Abstract
Purpose
To assess the patient experience and satisfaction after the implementation in routine of a personalized, digital programme before and after same-day discharge (SDD) robot-assisted radical prostatectomy (RARP).

Methods
The study is a pre/post-interventional, multi-surgeon, unicentre, prospective study. All consecutive patients undergoing SDD RARP were included during a 6-month period. After a pre-interventional assessment of the satisfaction rate (n = 26), all patients (n = 46) were introduced to the Betty. Care platform and followed the BETTY COACHING programme which included a specific radical prostatectomy module. The primary endpoint was patient satisfaction 6 weeks after SDD RARP. Secondary endpoints were hospital stay, readmission and complications rates, unplanned visits, and remote monitoring data.

Results
Median age and PSA were 66 years and 7.0 ng/ml. Lymph node-dissection and nerve-sparing procedures were performed in 41.3 and 87.0% of patients, respectively. Median operative time and blood loss were 80 min and 150 ml, respectively. The 90-day rates of unplanned visits, readmission and complications were improved after the digital tool implementation (2.2, 2.2, and 8.7%, respectively). Mean satisfaction score was 9.6 out of 10 (8.0 before implementation). Median duration of pain was 2 days after discharge, with median pain intensity of 2/10. Median duration of daily active use of remote monitoring was 34 days. The urinary continence rate was 91.3% 6 weeks after surgery in the postinterventional cohort.

Conclusions
The implementation of a personalized, surgery-specific, digital programme combining prehabilitation, patient education, rehabilitation, patient-reported outcome measurement and remote monitoring, improves patient experience and satisfaction and could help promoting early discharge even after a major surgery.
Partitions of Vertices and Facets in Trees and Stacked Simplicial Complexes
Authors: Fløystad Gunnar 
Published: June 2024

AbstractFor stacked simplicial complexes, (special subclasses of such are: trees, triangulations of polygons, stacked polytopes with their triangulations), we give an explicit bijection between partitions of facets (for trees: edges), and partitions of vertices into independent sets. More generally, we give bijections between facet partitions whose parts have minimal distance $$\ge s$$

≥
s

and vertex partitions whose parts have minimal distance $$\ge s+1$$

≥
s
+
1

.
The impact of interrupted corneal collagen crosslinking (CXL) treatment
Authors: Tholvsen Marit , Makdoumi Karim 
Published: June 2024

Abstract
Purpose
To evaluate progression of keratoconus in patients where CXL treatment was interrupted due to insufficient swelling of the cornea.

Methods
A retrospective review was conducted of all patients with keratoconus diagnosis who underwent CXL at the Department of Ophthalmology, Örebro University Hospital (USÖ) during the years 2010–2017. In total 377 eyes of 280 patients were screened for inclusion. In 17 eyes (15 patients), the treatment was interrupted due to insufficient swelling of the cornea. Patient journals were reviewed and keratometry examinations were analysed for long-term progression.

Results
Eleven eyes (nine patients) were included in the study. Five eyes showed no signs of progression after the interrupted CXL treatment. In one eye progression continued, however, first after a period of a number of years, indicating a delayed course of clinical progression.

Conclusion
This study indicates that debridement of the corneal epithelium and riboflavin administration without intense UVA radiation may slow or arrest the progression of keratoconus, likely due to photosensitisation from ambient light.
Extra-islet cells expressing insulin or glucagon in the pancreas of young organ donors
Authors: Granlund Louise , Korsgren Olle , Skog Oskar , Lundberg Marcus 
Published: June 2024

Abstract
Aims
The existence of insulin- or glucagon-expressing extra-islet endocrine cells scattered in the pancreas is well-known, but they have been sparsely characterized. The aim of this study was to examine their density, distribution, transcription-factor expression, and mitotic activity in young non-diabetic subjects.

Methods
Multispectral imaging was used to examine PDX1, ARX, Ki67, insulin and glucagon in extra-islet endocrine cells in pancreatic tissue from organ donors aged 1–25 years.

Results
Extra-islet insulin- or glucagon-positive cells were frequent in all donors (median 17.3 and 22.9 cells/mm2 respectively), with an insulin:glucagon cell ratio of 0.9. The density was similar regardless of age. PDX1 localized mainly to insulin-, and ARX mainly to glucagon-positive cells but, interestingly, many of the cells were negative for both transcription factors. Double-hormone-positive cells were rare but found in all age groups, as were insulin-positive cells expressing ARX and glucagon-positive cells expressing PDX1. Extra-islet endocrine cells with Ki67 expression were present but rare (0–2%) in all age groups.

Conclusions
Extra-islet endocrine cells are more frequent than islets. The preserved extra-islet cell density during pancreas volume-expansion from childhood- to adulthood indicates that new cells are formed, possibly from replication as cells with mitotic activity were discovered. The lack of transcription-factor expression in many cells indicates that they are immature, newly formed or plastic. This, together with the mitotic activity, suggests that these cells could play an important role in the expansion of beta-cell mass in situations of increasing demand, or in the turnover of the endocrine cell population.
Insights into Predicting Tooth Extraction from Panoramic Dental Images: Artificial Intelligence vs. Dentists
Authors: Motmaen Ila , Xie Kunpeng , Schönbrunn Leon , Berens Jeff , Grunert Kim , Plum Anna Maria , Raufeisen Johannes , Ferreira André , Hermans Alexander , Egger Jan , Hölzle Frank , Truhn Daniel , Puladi Behrus 
Published: June 2024

Abstract
Objectives
Tooth extraction is one of the most frequently performed medical procedures. The indication is based on the combination of clinical and radiological examination and individual patient parameters and should be made with great care. However, determining whether a tooth should be extracted is not always a straightforward decision. Moreover, visual and cognitive pitfalls in the analysis of radiographs may lead to incorrect decisions. Artificial intelligence (AI) could be used as a decision support tool to provide a score of tooth extractability.

Material and methods
Using 26,956 single teeth images from 1,184 panoramic radiographs (PANs), we trained a ResNet50 network to classify teeth as either extraction-worthy or preservable. For this purpose, teeth were cropped with different margins from PANs and annotated. The usefulness of the AI-based classification as well that of dentists was evaluated on a test dataset. In addition, the explainability of the best AI model was visualized via a class activation mapping using CAMERAS.

Results
The ROC-AUC for the best AI model to discriminate teeth worthy of preservation was 0.901 with 2% margin on dental images. In contrast, the average ROC-AUC for dentists was only 0.797. With a 19.1% tooth extractions prevalence, the AI model's PR-AUC was 0.749, while the dentist evaluation only reached 0.589.

Conclusion
AI models outperform dentists/specialists in predicting tooth extraction based solely on X-ray images, while the AI performance improves with increasing contextual information.

Clinical relevance
AI could help monitor at-risk teeth and reduce errors in indications for extractions.
Impact of the ventral hernia working group’s publication: a bibliometric analysis
Authors: Maskal Sara M. , de Figueiredo Sergio Mazzola Poli , Weaver Matthew , Schleicher Mary , Tu Chao , Ellis Ryan C. , Woo Kimberly , Fafaj Aldo , Remulla Daphne , Miller Benjamin T. , Petro Clayton C. , Beffa Lucas R.A. , Prabhu Ajita S. , Rosen Michael J. 
Published: June 2024

Abstract
Purpose
The Ventral Hernia Working Group (VHWG) proposed a ventral hernia grading guideline, primarily supported by expert opinion, recommending biologic mesh placement in high-risk patients. We investigated the relationship between this industry-sponsored guideline and discourse around ventral hernia repair (VHR).

Methods
Medline platform from Web of Science’s database identified publications “pre-VHWG”(1999-01-01 to 2009-12-31), and “post-VHWG”(2010-01-01 to 2020-12-31) describing VHR and complications or recurrence of VHR with the following comorbidities: COPD, smoking, diabetes, immunosuppression, or obesity. Poisson regression analyzed keyword frequency over time using logarithmically transformed data.

Results
Of 1291 VHR publications identified pre-VHWG and 3041 publications identified post-VHWG, 172 (13.3%) and 642 (21.1%) publications respectively included prespecified keywords. The keyword groups “biologic”(IRR 3.39,95%CI1.34-11.4,p = 0.022) and “comorbid”(IRR 1.95, 95%CI1.09-3.74,p = 0.033) significantly increased with frequency after publication of the VHWG.

Conclusion
The VHWG publication likely contributed to a focus on comorbidities and biologic mesh in the ensuing literature within the field of VHR.
Refined implicit characterization of engineering geology with uncertainties: a divide-and-conquer tactic-based approach
Authors: Li Mingchao , Chen Chuangwei , Liang Hui , Han Shuai , Ren Qiubing , Li Heng 
Published: June 2024

AbstractIn engineering geology, a reasonable assessment of the spatial distribution of uncertainty in a region is vital in guiding research, saving money, and shortening the period. However, the traditional modeling process requires a lot of manual interaction, and the uncertainty of the geological model cannot be accurately quantified and utilized. This paper proposes a novel implicit geological modeling and uncertainty analysis approach based on the triangular prism blocks, which is divided into data point acquisition, ensemble model with divide-and-conquer tactic (EMDCT), uncertainty analysis, and post-processing. By employing machine learning algorithms, the EMDCT gives superior results for implicit modeling. The sensitivity analysis of the prediction results is further evaluated via information entropy. According to the distribution of uncertainty, supplementary boreholes are selected as additional knowledge to retrain the local components of the model to enhance their performances. The implicit modeling method is applied to real hydraulic engineering problems by employing the EMDCT, and the proposed model has obvious advantages in the implicit geological characterization. The overall accuracy in the working area with sparse boreholes reaches 0.922, which is 0.013 higher than the traditional method. By evaluating the distribution of uncertainty, an accuracy of 0.962 can be achieved, which is equivalent to reducing 10 boreholes.
Can hair snares provide a reliable method for estimating abundance of an exotic ungulate?
Authors: Evans Tyler S. , Hileman Eric T. , Boudreau Melanie R. , Strickland Bronson K. , Iglay Raymond B. 
Published: June 2024

AbstractMitigating exotic species invasions requires reliable information on abundance and source-sink dynamics to evaluate effectiveness of control efforts. Despite being present in the southeastern United States for centuries, wild pigs (Sus scrofa) continue to invade new areas, including the Sam D. Hamilton Noxubee National Wildlife Refuge (NNWR) in Mississippi, a forested landscape with an emerging wild pig population. Considering the early stage of invasion, we designed and implemented a non-invasive hair sampling technique to estimate abundance and relatedness of wild pigs within the NNWR. Following 8-week sampling periods in 2021 (n = 160 samples) and 2022 (n = 159 samples), we identified 38 and 26 unique individuals, respectively, although recapture rates were too low to yield precise abundance estimates. We also identified low heterozygosity indicating high relatedness among our population which likely reflected lack of barrier features to gene flow and/or a limited number of external source populations. Finally, we observed positive trends in viable samples collected relative to sampling week, suggesting more optimal sampling period(s) may exist (e.g., autumn). We also acknowledge that use of grain in more accessible areas rather than our remote approach using scent-only attractants may increase hair snare attractiveness and wild pig encounters, while permitting increased sampling frequency. Collectively, these modifications (i.e., altered season, bait type, and access) provide viable pathways to increase precision for abundance estimates. When used concomitantly with ongoing monitoring and control efforts, non-invasive hair sampling will contribute to a more holistic understanding of this exotic species and better inform management actions.
Electrospun fiber materials based on polysaccharides and natural colorants for food packaging applications
Authors: Amorim Lúcia F. A. , Mouro Cláudia , Gouveia Isabel C. 
Published: June 2024

AbstractIn response to escalating concerns about environmental pollution from conventional plastic packaging, there is a growing interest in ecological and sustainable materials for food packaging. Consequently, biopolymer-based electrospun materials, particularly polysaccharides such as chitosan (CH), starch, and cellulose, have emerged as promising alternatives to synthetic ones due to their remarkable barrier properties and mechanical strength. Moreover, following the trend of employing polysaccharides as ecological and sustainable solutions for packaging materials, natural colorants have also been recognized as safer alternatives to synthetic dyes, addressing concerns about potential adverse effects on human health and the environment. These natural colorants add color to food packaging and enhance food freshness, possibly extending shelf life and promoting consumers' perception of food quality and safety. Therefore, this review summarizes recent advancements in fabricating and characterizing electrospun materials based on polysaccharides and natural colorants for sustainable food packaging. It explores various electrospinning techniques and highlights the incorporation of natural colorants, including bacterial pigments, for active and intelligent packaging applications. Moreover, it emphasizes the increasing role of the biobased industry in developing sustainable packaging materials with bioactive coloration. Lastly, the review addresses challenges, opportunities, and future research directions.
Graphical Abstract