bluuebunny/crossref_metadata_2025_split huggingface dataset:
Subcutaneous or intravenous administration of romiplostim in thrombocytopenic patients with lower risk myelodysplastic syndromes
Authors: Sekeres Mikkael A. , Kantarjian Hagop , Fenaux Pierre , Becker Pamela , Boruchov Adam , Guerci‐Bresler Agnes , Hu Kuolung , Franklin Janet , Wang Yow‐Ming C. , Berger Dietmar 
Published: October 2010

AbstractBACKGROUND:Romiplostim is a peptibody protein that augments thrombopoiesis by activating the thrombopoietin receptor.METHODS:In this phase 2, multicenter, open‐label study, 28 thrombocytopenic patients with lower risk myelodysplastic syndromes (MDS) were assigned to receive romiplostim 750 μg administered subcutaneously either weekly or biweekly or administered as biweekly intravenous injections for 8 weeks. Patients also could enter a 1‐year study extension phase.RESULTS:At least 1 adverse event was observed in 93% of patients. The most common adverse events were fatigue and headache (18% for both, and 5 events were grade 3 or 4. There was 1 serious treatment‐related adverse event in the biweekly intravenous cohort (hypersensitivity). This hypersensitivity resolved without discontinuation of study treatment. No patients developed neutralizing antibodies or bone marrow fibrosis. Of the patients who completed 8 weeks of treatment, 57% had a complete platelet response, an additional 8% had a major platelet response, and 61% did not require a platelet transfusion during this period. Weekly subcutaneous injections achieved the highest mean trough concentrations.CONCLUSIONS:The safety and efficacy profiles of romiplostim in this study suggested that weekly subcutaneous administration of 750 μg romiplostim is an appropriate starting dose for future clinical studies in patients with MDS and thrombocytopenia. Cancer 2011. © 2010 American Cancer Society.
Concordance in histological and biological parameters between first and second primary breast cancers
Authors: Huo Dezheng , Melkonian Stephanie , Rathouz Paul J. , Khramtsov Andrey , Olopade Olufunmilayo I. 
Published: October 2010

AbstractBACKGROUND:Women with breast cancer are more likely to have a second breast cancer than women in the general population are to have a primary cancer. However, the biological relationship between primary and second breast cancers is not clear.METHODS:A total of 30,617 patients diagnosed with bilateral breast cancers between 1990 and 2007 were identified through 17 cancer registries of the Surveillance, Epidemiology, and End Results program. Logistic regression with odds ratios (ORs) and 95% confidence intervals (CIs) was used to model strength of association in hormone receptor status, grade, and histology between 2 cancers.RESULTS:There was a strong association in estrogen receptor status between 2 bilateral tumors (OR, 7.64; 95% CI, 7.00‐8.35). The strength of association in estrogen receptor status depended on the time interval between the first and second tumors and age at diagnosis. The OR was 25.9 for synchronous tumors (within 1 month) and 3.69 for metachronous tumors separated by ≥10 years. The strength of association was stronger in patients whose first cancer was diagnosed before age 50 (OR, 11.7) versus after age 50 (OR, 5.71). A similar pattern was observed for progesterone receptor, grade, and histological type, but with relatively weaker association.CONCLUSIONS:The strong concordance in hormone receptor status of primary and second breast cancers suggests that 2 breast cancers arise in a common milieu and that tumor subtypes are predetermined in the early stage of breast carcinogenesis. Cancer 2011. © 2010 American Cancer Society.
Long‐term outcomes in the second‐line treatment of chronic myeloid leukemia
Authors: Jabbour Elias , Cortes Jorge , Kantarjian Hagop 
Published: October 2010

AbstractChronic myeloid leukemia (CML) is a progressive and often fatal myeloproliferative neoplasm. The hallmark of CML is an acquired chromosomal translocation known as the Philadelphia chromosome (Ph), which results in the synthesis of the breakpoint cluster region‐Abelson murine leukemia (BCR‐ABL) fusion oncoprotein, a constitutively active tyrosine kinase. The introduction of imatinib, a tyrosine kinase inhibitor (TKI) that is specific for BCR‐ABL, was a major breakthrough in CML therapy. Although most patients respond to first‐line imatinib therapy, some experience loss of response (resistance) or require treatment discontinuation because of toxicity (intolerance). For patients with CML, failure on standard‐dose imatinib therapy (400 mg daily), imatinib dose escalation (600‐800 mg daily) is a second‐line option. However, high‐dose imatinib is not an appropriate approach for patients who experience drug toxicity, and there remain questions over the durability of responses achieved with this strategy. Alternative second‐line options include the TKIs dasatinib and nilotinib. A substantial amount of long‐term data for these agents is available. Although both are potent and specific BCR‐ABL TKIs, dasatinib and nilotinib exhibit unique pharmacologic profiles and response patterns relative to different patient characteristics, such as disease stage and BCR‐ABL mutation status. To optimize therapeutic benefit, clinicians should select treatment based on each patient's historic response, adverse‐event tolerance, and risk factors. Cancer 2010. © 2010 American Cancer Society.
Fine‐needle aspiration cytopathology—An accurate diagnostic modality in mammary carcinoma with osteoclast‐like giant cells
Authors: Jacquet Solène‐Florence , Balleyguier Corinne , Garbay Jean‐Rémi , Bourgier Céline , Mathieu Marie‐Christine , Delaloge Suzette , Vielh Philippe 
Published: October 2010

AbstractBACKGROUND:Invasive ductal carcinoma with osteoclast‐like giant cells (OGCs) is a very rare breast tumor the main characteristic of which is the presence of multinucleated cells of histiocytic nature.METHODS:The authors report a study of 8 consecutive cases of fine‐needle aspiration cytopathology (FNAC) of breast nodules in which OGCs and malignant epithelial cells were associated and diagnosed as mammary carcinoma with OGCs. These cases were selected over a period of 5 years from more than 6000 patients who were examined during a weekly, single‐day, multidisciplinary breast clinic. The corresponding biopsies and surgical specimens were examined histologically in an immunohistochemical study using a histiocytic marker (cluster of differentiation 68 [CD68]).RESULTS:Conventional histologic analysis made it possible to diagnose 5 of the 8 cases as mammary carcinoma with OGCs; whereas, in the other 3 cases, OGCs were not detected without the help of immunohistochemistry.CONCLUSIONS:FNAC appeared to be a very efficient way to diagnose breast carcinoma with OGCs, because it detected forms with only a few OGCs that usually are not observed at histologic diagnosis. Consequently, the current results indicated that mammary carcinoma with OGCs may be more frequent than reported previously. Cancer (Cancer Cytopathol) 2010. © 2010 American Cancer Society.
Hydrogen Sulfide Adsorption on MOFs and MOF/Graphite Oxide Composites
Authors: Petit Camille , Mendoza Barbara , Bandosz Teresa J. 
Published: October 2010

AbstractComposites of a copper‐based metal‐organic framework (MOF) and graphite oxide (GO) were tested for hydrogen sulfide removal at ambient conditions. In order to understand the mechanisms of adsorption, the initial and exhausted samples were analyzed by various techniques including X‐ray diffraction, Fourier transform infrared spectroscopy, thermogravimetric analyses, and sorption of nitrogen. Compared to the parent materials, an enhancement in hydrogen sulfide adsorption was found. It was the result of physical adsorption of water and H2S in the pore space formed at the interface between the MOF units and the graphene layers where the dispersive forces are the strongest. Besides physisorption, reactive adsorption was found as the main mechanism of retention. H2S molecules bind to the copper centers of the MOF. They progressively react with the MOF units resulting in the formation of copper sulfide. This leads to the collapse of the MOF structure. Water enhances adsorption in the composites as it allows the dissolution of hydrogen sulfide.
Parental division of labor, coordination, and the effects of family structure on parenting in monogamous prairie voles (Microtus ochrogaster)
Authors: Ahern Todd H. , Hammock Elizabeth A.D. , Young Larry J. 
Published: October 2010

AbstractFamily relationships help shape species‐typical social and emotional development, but our understanding of how this shaping occurs is still relatively limited. Prairie voles are a socially monogamous and biparental species that is well situated to complement traditional animal models, such as rats and mice, in investigating the effects of family experience. In this series of studies, we aimed to test hypotheses relating to how prairie vole families function under undisturbed, standard laboratory conditions. In the first study, we compared the parental behavior of primiparous biparental (BP) and single‐mother (SM) prairie vole family units for 12 postnatal days and then tested for sex differences, behavioral coordination, and family structure effects. Under BP conditions, nest attendance was coordinated and shared equally by both sexes, while pup‐directed and partner‐directed licking and grooming (LG) were coordinated in a sex and social‐context‐dependent manner. Contrary to our expectations, SMs showed no evidence of strong parental compensation in response to the lack of the father, indicating a minimal effect of family structure on maternal behavior but a large effect on pup care. In the second study, we examined the effects of these BP and SM rearing conditions on family dynamics in the next generation and found that SM‐reared adult parents exhibited lower rates of pup‐directed LG in comparison to BP‐reared counterparts. Situated in the context of human family dynamics and psychology, these results suggest that the study in prairie voles may help improve our understanding of family systems and how perturbations to these systems can affect adults and offspring. © 2010 Wiley Periodicals, Inc. Dev Psychobiol 53:118–131, 2011
Age‐related changes in visual recognition memory during infancy and early childhood
Authors: Morgan Kirstie , Hayne Harlene 
Published: October 2010

AbstractAge‐related changes in long‐term memory during infancy and early childhood were examined using the Visual Recognition Memory (VRM) procedure. Independent groups of 1‐, 2‐, 3‐, and 4‐year‐olds were familiarized with a visual stimulus and were tested either immediately or after a delay that ranged from 24 hr to 6 months. Although all age groups exhibited a significant novelty preference when tested immediately after familiarization, clear age‐related differences emerged over longer retention intervals. We conclude that age‐related increases in basic retention are a fundamental aspect of mammalian memory development and, in humans, these increases may play a vital role in the offset of childhood amnesia. © 2010 Wiley Periodicals, Inc. Dev Psychobiol 53:157–165, 2011
Rhoh deficiency reduces peripheral T‐cell function and attenuates allogenic transplant rejection
Authors: Porubsky Stefan , Wang Shijun , Kiss Eva , Dehmel Stefan , Bonrouhi Mahnaz , Dorn Tatjana , Luckow Bruno , Brakebusch Cord , Gröne Hermann‐Josef 
Published: October 2010

AbstractRhoh is a hematopoietic system‐specific GTPase. Rhoh‐deficient T cells have been shown to have a defect in TCR signaling manifested during their thymic development. Our aims were to investigate the phenotype of peripheral Rhoh‐deficient T cells and to explore in vivo the potential benefit of Rhoh deficiency in a clinically relevant situation, in which T‐cell inhibition is desirable. In murine allogenic kidney transplantation, Rhoh deficiency caused a significant 75% reduction of acute and chronic transplant rejection accompanied by 75% lower alloantigen‐specific antibody levels and significantly better graft function. This effect was independent of the lower T‐cell numbers in Rhoh‐deficient recipients, because injection of equal numbers of Rhoh‐deficient or control T cells into kidney transplanted mice with SCID led again to a significant 60% reduction of rejection. Mixed lymphocyte reaction revealed that the weaker alloreactivity was associated with a 85% lower cytotoxicity and a 50–80% lower cytokine release in Rhoh‐deficient T cells without an influence on the secretion itself. Antigen uptake and presentation in DC were unaffected by Rhoh deficiency. These findings stress the importance of Rhoh for the function of peripheral T cells.
CD4+CD25+ Treg regulate the contribution of CD8+ T‐cell subsets in repopulation of the lymphopenic environment
Authors: Almeida Afonso R. M. , Ciernik Ilja F. , Sallusto Federica , Lanzavecchia Antonio 
Published: October 2010

AbstractPeripheral T‐cell expansion is of major relevance for immune function after lymphopenia. In order to promote regeneration, the process should result in a peripheral T‐cell pool with a similar subpopulation structure as before lymphopenia. We investigated the repopulation of the CD8+ central‐memory T cells (TCM) and effector‐memory T cells (TEM) pools after adoptive transfer of sorted CD8+ T cells from naïve, TCM and TEM subsets into T‐cell‐deficient hosts. We show that the initial kinetics of expansion are distinct for each subset and that the contribution to the repopulation of the CD8+ T‐cell pool by the progeny of each subset is not a mere function of its initial expansion. We demonstrate that CD4+CD25+ Treg play a major role in the repopulation of the CD8+ T‐cell pool and that CD8+ T‐cell subsets impact on each other. In the absence of CD4+CD25+ Treg, a small fraction of naïve CD8+ T cells strongly proliferates, correlating with further expansion and differentiation of co‐expanding CD8+ T cells. CD4+CD25+ Treg suppress these responses and lead to controlled repopulation, contributing decisively to the maintenance of recovered TCM and TEM fractions, and leading to repopulation of each pool with progeny of its own kind.
Genetic proof for the transient nature of the Th17 phenotype
Authors: Kurschus Florian C. , Croxford Andrew L. , P. Heinen André , Wörtge Simone , Ielo Daniele , Waisman Ari 
Published: October 2010

AbstractIL‐17‐producing CD4+ T cells (Th17) have been classified as a new T helper cell subset. Using an IL‐17 fate mapping mouse strain, which genetically fixes the memory of IL‐17 expression, we demonstrate that IL‐17A/F‐expressing T helper cells generated either in vitro or in vivo are not a stable T‐cell subset. Upon adoptive transfer of IL‐17F‐reporter‐positive Th17 cells to RAG‐deficient or WT animals, encephalitogenic Th17 cells partially lose IL‐17 expression and upregulate IFN‐γ. Additionally, we show that Th1 cells can convert in vivo to IL‐17A/IFN‐γ‐coexpressing cells in the mesenteric lymph nodes (mLN). Our data classify IL‐17A and IL‐17F as cytokines produced transiently in response to the local microenvironment, thus showing that IL‐17 expression does not define an end‐stage T helper cell subset.
First Examples of α‐(1→4)‐Glycosylation Reactions on Ionic Liquid Supports
Authors: Pépin Matthieu , Hubert‐Roux Marie , Martin Claudette , Guillen Fréderic , Lange Catherine , Gouhier Géraldine 
Published: October 2010

AbstractWe have developed the first α‐(1→4)‐glycosylation reactions on ionic liquid supports. The purification steps involve simple liquid/liquid extractions. The positive influence of the soluble support on the stereoselectivity was proved, and the recyclable character of the ionic liquid was demonstrated.
Manipulating particles in microfluidics by floating electrodes
Authors: Yalcin Sinan E. , Sharma Ashutosh , Qian Shizhi , Joo Sang W. , Baysal Oktay 
Published: October 2010

AbstractVarious particle manipulations including enrichment, movement, trapping, separation, and focusing by floating electrodes attached to the bottom wall of a straight microchannel under an imposed DC electric field have been experimentally demonstrated. In contrast to a dielectric microchannel possessing a nearly uniform surface charge (or ζ potential), the metal strip (floating electrode) is polarized under the imposed electric field, resulting in a nonuniform distribution of the induced surface charge with a zero net surface charge along the floating electrode's surface, and accordingly induced‐charge electroosmotic flow near the metal strip. The induced induced‐charge electroosmotic flow can be regulated by controlling the strength of the imposed electric field and affects both the hydrodynamic field and the particle's motion. By using a single floating electrode, charged particles could be locally concentrated in a section of the channel or in an end‐reservoir and move toward either the anode or the cathode by controlling the strength of the imposed electric field. By using double floating electrodes, negatively charged particles could be concentrated between the floating electrodes, subsequently squeezed to a stream flowing in the center region of the microchannel toward the cathodic reservoir, which can be used to focus particles.
Is bisphenol A a weak carcinogen like the natural estrogens and diethylstilbestrol?
Authors: Cavalieri Ercole L. , Rogan Eleanor G. 
Published: October 2010

AbstractBisphenol A (BPA) displays weak estrogenic properties and could be a weak carcinogen by a mechanism similar to that of estrone (E1), estradiol (E2) and the synthetic estrogen diethylstilbestrol, a human carcinogen. A wide variety of scientific evidence supports the hypothesis that certain estrogen metabolites, predominantly catechol estrogen‐3,4‐quinones, react with DNA to cause mutations that can lead to the initiation of cancer. One of the major pathways of estrogen metabolism leads to the 4‐catechol estrogens, 4‐OHE1(E2), which are oxidized to their quinones, E1(E2)‐3,4‐Q. The quinones react with DNA to form predominantly the depurinating adducts 4‐OHE1(E2)‐1‐N3Ade and 4‐OHE1(E2)‐1‐N7Gua. This process constitutes the predominant pathway in the initiation of cancer by estrogens. One pathway of BPA metabolism is hydroxylation of one of its symmetric benzene rings to form its catechol, 3‐OHBPA. Subsequent oxidation to BPA‐3,4‐quinone would lead to reaction with DNA to form predominantly the depurinating adducts 3‐OHBPA‐6‐N3Ade and 3‐OHBPA‐6‐N7Gua. The resulting apurinic sites in the DNA could generate mutations in critical genes that can initiate human cancers. The catechol of BPA may also alter expression of estrogen‐activating and deactivating enzymes, and/or compete with methoxylation of 4‐OHE1(E2) by catechol‐O‐methyltransferase, thereby unbalancing the metabolism of estrogens to increase formation of E1(E2)‐3,4‐Q and the depurinating estrogen‐DNA adducts leading to cancer initiation. Thus, exposure to BPA could increase the risk of developing cancer by direct and/or indirect mechanisms. Knowledge of these mechanisms would allow us to begin to understand how BPA may act as a weak carcinogen and would be useful for regulating its use. © 2010 IUBMB IUBMB Life, 62(10): 746–751, 2010
TIEG1 negatively controls the myoblast pool indispensable for fusion during myogenic differentiation of C2C12 cells
Authors: Miyake Masato , Hayashi Shinichiro , Iwasaki Shunsuke , Uchida Takafumi , Watanabe Kouichi , Ohwada Shyuichi , Aso Hisashi , Yamaguchi Takahiro 
Published: October 2010

AbstractThe transforming growth factor (TGF)‐β inducible early gene (TIEG)‐1 is implicated in the control of cell proliferation, differentiation, and apoptosis in some cell types. Since TIEG1 functioning may be associated with TGF‐β, a suppressor of myogenesis, TIEG1 is also likely to be involved in myogenesis. Therefore, we investigated the function of TIEG1 during myogenic differentiation in vitro using the murine myoblasts cell line, C2C12. TIEG1 expression increased during differentiation of C2C12 cells. Constitutive expression of TIEG1 reduced survival and decreased myotube formation. Conversely, knocking down TIEG1 expression increased the number of viable cells during differentiation, and accelerated myoblast fusion into multinucleated myotubes. However, expression of the myogenic differentiation marker, myogenin, remained unaffected by TIEG1 knockdown. The mechanism underlying these events was investigated by focusing on the regulation of myoblast numbers after induction of differentiation. The knockdown of TIEG1 led to changes in cell cycle status and inhibition of apoptosis during the initial stages of differentiation. Microarray and real‐time PCR analyses showed that the regulators of cell cycle progression were highly expressed in TIEG1 knockdown cells. Therefore, TIEG1 is a negative regulator of the myoblast pool that causes inhibition of myotube formation during myogenic differentiation. J. Cell. Physiol. 226: 1128–1136, 2011. © 2010 Wiley‐Liss, Inc.
The hippo pathway in biological control and cancer development
Authors: Chan Siew Wee , Lim Chun Jye , Chen Liming , Chong Yaan Fun , Huang Caixia , Song Haiwei , Hong Wanjin 
Published: October 2010

AbstractThe Hippo pathway is an evolutionally conserved protein kinase cascade involved in regulating organ size in vivo and cell contact inhibition in vitro by governing cell proliferation and apoptosis. Deregulation of the Hippo pathway is linked to cancer development. Its first core kinase Warts was identified in Drosophila more than 15 years ago, but it gained much attention when other core components of the pathway were identified 8 years later. Major discoveries of the pathway were made during past several years. The core kinase components Hippo, Salvador, Warts, and Mats in the fly and Mst1/2, WW45, Lats1/2, and Mob1 in mammals phosphorylate and inactivate downstream transcriptional co‐activators Yorkie in the fly, Yes‐associated protein (YAP) and transcriptional co‐activator with PDZ‐binding motif (TAZ) in mammals, respectively. Phosphorylated Yorkie, YAP, and TAZ are sequestered in the cytoplasm by interaction with 14‐3‐3 proteins. Here we review recent progresses of this pathway by focusing on how these proteins communicate with each other and how loss of regulation results in cancers. J. Cell. Physiol. 226: 928–939, 2011. © 2010 Wiley‐Liss, Inc.