bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reusable Framework for Remote Monitoring and Maintenance of Manufacturing Systems
Authors: Fayolle Jacques , Gravier Christophe , Ates Mia 
Published: November 2009

The aim of this paper is to address the problem of remote control of manufacturing systems for maintenance, object conception and production purposes.
We present a global approach of this problem according to two research axis: the genericity of the platform according to the underlying device, and the security of the data and information systems. The primary goal of this paper is to describe the framework that allows the remote control, and especially its independence to the devices. We propose a special study on the security issues in such architecture by explaining why we have made the choice of adopting identity federation technologies as a mean to reach remote devices hosted in different Information Systems. We also describe how this kind of systems can be used in a collaborative way in order to build a real extended entreprise for e-manufacturing systems.
The Heavy Chain Variable Segment Gene Repertoire in Chronic Chagas′ Heart Disease
Authors: Grippo Vanina , Mahler Evelyn , Elias Fernando E. , Cauerhff Ana , Gómez Karina A. , Tentori Maria C. , Ruiz Aurora , Vigliano Carlos A. , Laguens Ruben P. , Berek Claudia , Levin Mariano J. 
Published: November 2009

AbstractPatients chronically infected with Trypanosoma cruzi develop chronic Chagas′ heart disease (cChHD). Their Ab response is suspected to be involved in the cardiac pathogenesis. Reactivity of serum Abs from these patients has been extensively studied but little is known about the diversity of the in vivo IgG repertoire. We analyzed 125 variable H chain (VH) genes and compared it to repertoires from healthy individuals, and patients with autoimmune processes and other infections. VH were from plasma cells isolated from heart tissue of three cChHD patients and from a Fab combinatorial library derived from bone marrow of another cChHD patient. The role of the parasite in shaping the Ab repertoire was assessed analyzing VH genes before and after panning against T. cruzi Ag. Among recovered VH genes, a significantly increased representation of VH4 was observed. Plasma cells at the site of cardiac infiltration showed an increased VH1 usage. CDR3 lengths were similar to the ones found in the healthy repertoire and significantly shorter than in other infections. VH derived from anti-T. cruzi Fab and plasma cells showed a higher proportion of hypermutated genes, 46.9% and 43.75%, respectively, vs 30.9% of the cChHD patient repertoire, pointing to the role of parasite Ags in the shaping of the humoral response in Chagas′ disease. No histological evidence of germinal center-like structures was observed in heart tissue. In accordance, VH analysis of heart plasmocytes revealed no evidence of clonal B cell expansion, suggesting that they migrated into heart tissue from secondary lymphoid organs.
The Runx3 Transcription Factor Augments Th1 and Down-Modulates Th2 Phenotypes by Interacting with and Attenuating GATA3
Authors: Kohu Kazuyoshi , Ohmori Hidetaka , Wong Won Fen , Onda Daisuke , Wakoh Takeshi , Kon Shunsuke , Yamashita Masakatsu , Nakayama Toshinori , Kubo Masato , Satake Masanobu 
Published: November 2009

AbstractRecently, it was reported that the expression of Runt-related transcription factor 3 (Runx3) is up-regulated in CD4+ helper T cells during Th1 cell differentiation, and that Runx3 functions in a positive feed-forward manner with the T-box family transcription factor, T-bet, which is a master regulator of Th1 cell differentiation. The relative expression levels of IFN-γ and IL-4 are also regulated by the Th2-associated transcription factor, GATA3. Here, we demonstrate that Runx3 was induced in Th2 as well as Th1 cells and that Runx3 interacted with GATA3 and attenuated GATA3 transcriptional activity. Ectopic expression of Runx3 in vitro in cultured cells or transgenic expression of Runx3 in mice accelerated CD4+ cells to a Th1-biased population or down-modulated Th2 responses, in part by neutralizing GATA3. Our results suggest that the balance of Runx3 and GATA3 is one factor that influences the manifestation of CD4+ cells as the Th1 or Th2 phenotypes.
Tumor Progression Locus 2 (Map3k8) Is Critical for Host Defense againstListeria monocytogenesand IL-1β Production
Authors: Mielke Lisa A. , Elkins Karen L. , Wei Lai , Starr Robyn , Tsichlis Philip N. , O'Shea John J. , Watford Wendy T. 
Published: November 2009

AbstractTumor progression locus 2 (Tpl2, also known as Map3k8 and Cot) is a serine-threonine kinase critical in innate immunity, linking toll-like receptors (TLRs) to TNF production through its activation of ERK. Tpl2−/− macrophages have abrogated TNF production but overproduce IL-12 in response to TLR ligands. Despite enhanced IL-12 production, Tpl2−/− T cells have impaired IFN-γ production. Therefore, the role of Tpl2 in a bona fide bacterial infection where all of these cytokines are important in host defense is unclear. To address this issue, we infected Tpl2−/− mice with the model pathogen Listeria monocytogenes. We found that Tpl2−/− mice infected i.v. with L. monocytogenes had increased pathogen burdens compared with wild-type mice and rapidly succumbed to infection. Enhanced susceptibility correlated with impaired signaling through TLR2 and nucleotide-binding oligomerization domain 2, two receptors previously shown to mediate Listeria recognition. Surprisingly, TNF production in response to infection was not significantly impaired, even though Tpl2 has been implicated in the regulation of TNF. We found that the role of Tpl2 has cell-type specific effects in regulating TNF and transduces signals from some, but not all, pattern recognition receptors (PRR). In contrast to the cell-type- and receptor-specific regulation of TNF, we found that Tpl2 is essential for IL-1β production from both macrophages and dendritic cells. These studies implicate Tpl2 as an important mediator for collaboration of pattern recognition receptors with danger-associated molecular patterns to induce TNF and IL-1β production and optimal host defense.
In a Murine Tuberculosis Model, the Absence of Homeostatic Chemokines Delays Granuloma Formation and Protective Immunity
Authors: Khader Shabaana A. , Rangel-Moreno Javier , Fountain Jeffrey J. , Martino Cynthia A. , Reiley William W. , Pearl John E. , Winslow Gary M. , Woodland David L. , Randall Troy D. , Cooper Andrea M. 
Published: November 2009

AbstractMycobacterium tuberculosis infection (Mtb) results in the generation of protective cellular immunity and formation of granulomatous structures in the lung. CXCL13, CCL21, and CCL19 are constitutively expressed in the secondary lymphoid organs and play a dominant role in the homing of lymphocytes and dendritic cells. Although it is known that dendritic cell transport of Mtb from the lung to the draining lymph node is dependent on CCL19/CCL21, we show in this study that CCL19/CCL21 is also important for the accumulation of Ag-specific IFN-γ-producing T cells in the lung, development of the granuloma, and control of mycobacteria. Importantly, we also show that CXCL13 is not required for generation of IFN-γ responses, but is essential for the spatial arrangement of lymphocytes within granulomas, optimal activation of phagocytes, and subsequent control of mycobacterial growth. Furthermore, we show that these chemokines are also induced in the lung during the early immune responses following pulmonary Mtb infection. These results demonstrate that homeostatic chemokines perform distinct functions that cooperate to mediate effective expression of immunity against Mtb infection.
Does drawing facilitate older children's reports of emotionally laden events?
Authors: Patterson Tess , Hayne Harlene 
Published: November 2009

AbstractAlthough it is well‐established that drawing about an event increases the amount of verbal information that young children provide during an interview, it is unclear whether drawing continues to facilitate children's reports as they get older. In the present experiment, 90 children, ranging from 5‐ to 12‐years old, were asked to draw and tell or to just tell about emotional events they had experienced. Children of all ages reported more information when asked to draw and tell rather than to tell only. Drawing had no negative effect on the accuracy of children's accounts. Drawing also increased the number of open‐ended questions and minimal responses that interviewers used. We conclude that drawing may be a useful tool in clinical and forensic settings with children of all ages; it increases the amount of information that children report and the number of appropriate questions that interviewers ask. Copyright © 2009 John Wiley & Sons, Ltd.
Microstructure and Texture Formation During Near Conventional Forging of an Intermetallic Ti–45Al–5Nb Alloy
Authors: Stark Andreas , Bartels Arno , Clemens Helmut , Kremmer Sascha , Schimansky Frank‐Peter , Gerling Rainer 
Published: November 2009

AbstractTexture formation was studied in an intermetallic Ti‐45at%Al‐5at%Nb alloy after uniaxial compression and near conventional forging. Depending on the deformation conditions the texture of the γ‐TiAl phase is formed by pure deformation components, components related to dynamic recrystallization, or transformation components. This changing corresponds with microstructural observations. The α2‐Ti3Al and the α‐Ti(Al) phase show a similar texture as it is known for Ti and Ti‐base alloys after compressive deformation at elevated temperatures. In contrast to the γ texture, no significant change of the α/α2 texture was observed in the temperature range between 800 °C and just below the α‐transus temperature (Tα = 1295 °C).
Rapid Generation of Biologically Relevant Hydrogels Containing Long‐Range Chemical Gradients
Authors: He Jiankang , Du Yanan , Villa‐Uribe José L. , Hwang Changmo , Li Dichen , Khademhosseini Ali 
Published: November 2009

AbstractMany biological processes are regulated by gradients of bioactive chemicals. Thus, the generation of materials with embedded chemical gradients may be beneficial for understanding biological phenomena and generating tissue‐mimetic constructs. Here a simple and versatile method to rapidly generate materials containing centimeter‐long gradients of chemical properties in a microfluidic channel is described. The formation of a chemical gradient is initiated by a passive‐pump‐induced forward flow and further developed during an evaporation‐induced backward flow. The gradient is spatially controlled by the backward flow time and the hydrogel material containing the gradient is synthesized via photopolymerization. Gradients of a cell‐adhesion ligand, Arg‐Gly‐Asp‐Ser (RGDS), are incorporated in poly(ethylene glycol)‐diacrylate (PEG‐DA) hydrogels to test the response of endothelial cells. The cells attach and spread along the hydrogel material in a manner consistent with the RGDS‐gradient profile. A hydrogel containing a PEG‐DA concentration gradient and constant RGDS concentration is also shown. The morphology of cells cultured on such hydrogel changes from round in the lower PEG‐DA concentration regions to well‐spread in the higher PEG‐DA concentration regions. This approach is expected to be a valuable tool to investigate the cell–material interactions in a simple and high‐throughput manner and to design graded biomimetic materials for tissue engineering applications.
A Simple and Innovative Route to Prepare a Novel Carbon Nanotube/Prussian Blue Electrode and its Utilization as a Highly Sensitive H2O2 Amperometric Sensor
Authors: Nossol Edson , Zarbin Aldo J.G. 
Published: November 2009

AbstractThe utilization of iron‐based species (mainly metallic iron, hematite and magnetite) encapsulated into multi‐walled carbon nanotubes (CNTs) as reactants in an electrochemical synthesis is reported for the first time in this work. Prussian blue (PB) is electrosynthesized in a heterogeneous reaction between ferricyanide ions in aqueous solution and the iron‐species encapsulated into CNTs, resulting in novel CNT/PB paste electrodes. This innovative preparation route produces an intimate contact between the PB and the CNTs, which improves the stability and redox properties of PB. The PB formation and the chemical interaction between the PB and the CNTs are confirmed by Raman spectroscopy. The electrode is employed as a hydrogen peroxide amperometric sensor, resulting in a very low limit of detection (1.94 × 10−8 mol L−1) and very high sensitivity (15.3 A cm−2 M−1).
A comparison of auditory brainstem responses and behavioral estimates of hearing sensitivity in Lemur catta and Nycticebus coucang
Authors: Ramsier Marissa A. , Dominy Nathaniel J. 
Published: November 2009

AbstractPrimates depend on acoustic signals and cues to avoid predators, locate food, and share information. Accordingly, the structure and function of acoustic stimuli have long been emphasized in studies of primate behavioral and cognitive ecology. Yet, few studies have addressed how well primates hear such stimuli; indeed, the auditory thresholds of most primate species are unknown. This empirical void is due in part to the logistic and economic challenges attendant on traditional behavioral testing methods. Technological advances have produced a safe and cost‐effective alternative—the auditory brainstem response (ABR) method, which can be utilized in field conditions, on virtually any animal species, and without subject training. Here we used the ABR and four methods of threshold determination to construct audiograms for two strepsirrhine primates: the ring‐tailed lemur (Lemur catta) and slow loris (Nycticebus coucang). Next, to verify the general efficacy of the ABR method, we compared our results to published behaviorally‐derived audiograms. We found that the four ABR threshold detection methods produced similar results, including relatively elevated thresholds but similarly shaped audiograms compared to those derived behaviorally. The ABR and behavioral absolute thresholds were significantly correlated, and the frequencies of best sensitivity and high‐frequency limits were comparable. However, at frequencies ≤2 kHz, ABR thresholds were especially elevated, resulting in decreased agreement with behavioral thresholds and, in Lemur, the ABR 10‐dB range starting points were more than 2 octaves higher than the behavioral points. Finally, a comparison of ABR‐ and behaviorally‐derived audiograms from various animal taxa demonstrates the widespread efficacy of the ABR for estimating frequency of best sensitivity, but otherwise suggests caution; factors such as stimulus properties and threshold definition affect results. We conclude that the ABR method is a promising technique for estimating primate hearing sensitivity, but that additional data are required to explore its efficacy for estimating low‐frequency thresholds. Am. J. Primatol. 72:217–233, 2010. © 2009 Wiley‐Liss, Inc.
Detection of access to terror‐related Web sites using an Advanced Terror Detection System (ATDS)
Authors: Elovici Yuval , Shapira Bracha , Last Mark , Zaafrany Omer , Friedman Menahem , Schneider Moti , Kandel Abraham 
Published: November 2009

AbstractTerrorist groups use the Web as their infrastructure for various purposes. One example is the forming of new local cells that may later become active and perform acts of terror. The Advanced Terrorist Detection System (ATDS), is aimed at tracking down online access to abnormal content, which may include terrorist‐generated sites, by analyzing the content of information accessed by the Web users. ATDS operates in two modes: the training mode and the detection mode. In the training mode, ATDS determines the typical interests of a prespecified group of users by processing the Web pages accessed by these users over time. In the detection mode, ATDS performs real‐time monitoring of the Web traffic generated by the monitored group, analyzes the content of the accessed Web pages, and issues an alarm if the accessed information is not within the typical interests of that group and similar to the terrorist interests. An experimental version of ATDS was implemented and evaluated in a local network environment. The results suggest that when optimally tuned the system can reach high detection rates of up to 100% in case of continuous access to a series of terrorist Web pages.
Assessing risk in adolescent sexual offenders: recommendations for clinical practice
Authors: Vitacco Michael J. , Caldwell Michael , Ryba Nancy L. , Malesky Alvin , Kurus Samantha J. 
Published: November 2009

AbstractAccurately predicting the likelihood that an adolescent with a sex offense history will reoffend is a precarious task that carries with it the potential for extreme consequences for the adolescent offender (e.g., lifelong public registration). Recently implemented laws regarding adolescent sex offenders are dramatically upstream of current knowledge. Several of these laws were ostensibly based on the misassumption that clinicians could accurately identify adolescents at the greatest risk for sexual recidivism. However, predicting which adolescents are at greatest risk to sexually recidivate is severely constrained by limited knowledge about which predictors are most accurately linked to sexual recidivism and uncertainty over how to best make use of instruments designed to predict recidivism. This paper reviews research on risk assessment and provides a set of recommendations for conducting risk assessments with adolescent sex offenders. Copyright © 2009 John Wiley & Sons, Ltd.
Regulation of XBP‐1 signaling during transient and stable recombinant protein production in CHO cells
Authors: Ku Sebastian C. Y. , Toh Poh Choo , Lee Yih Yean , Chusainow Janet , Yap Miranda G. S. , Chao Sheng‐Hao 
Published: November 2009

AbstractX‐box binding protein 1 (XBP‐1) is a key regulator of cellular unfolded protein response (UPR). The spliced isoform of XBP‐1, XBP‐1S, is a transcription activator, which is expressed only when UPR is induced. However, the impact of recombinant protein production on the regulation of XBP‐1 signaling in CHO cells is not well understood. In this report, we cloned the Chinese hamster XBP‐1 homolog to aid the investigation of the interplay between protein productivity, culture conditions, and endogenous XBP‐1 signaling in CHO cells. Interestingly, expression of XBP‐1S is detected in the non‐producing and unstressed CHO‐K1 cells. Transient expression of recombinant erythropoietin reveals a positive correlation between XBP‐1 mRNA abundance and protein production level. However, such a correlation is not observed in batch cultivation of stable producing cell lines. The increased XBP‐1 splicing is detected in late‐phase cultures, suggesting that induction of XBP‐1S may be a result of nutrient limitations or other environmental stresses rather than that of increased intracellular accumulation of recombinant proteins. Our data suggest that XBP‐1 is a key determinant for the secretory capacity of CHO cells. Understanding its dynamic regulation hence provides a rational basis for cellular engineering strategies to improve recombinant protein secretion. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Wet‐milling transgenic maize seed for fraction enrichment of recombinant subunit vaccine
Authors: Moeller Lorena , Taylor‐Vokes Raye , Fox Steve , Gan Qinglei , Johnson Lawrence , Wang Kan 
Published: November 2009

AbstractThe production of recombinant proteins in plants continues to be of great interest for prospective large‐scale manufacturing of industrial enzymes, nutrition products, and vaccines. This work describes fractionation by wet‐milling of transgenic maize expressing the B subunit of the heat‐labile enterotoxin of Escherichia coli (LT‐B), a potent immunogen and candidate for oral vaccine and vaccine components. The LT‐B gene was directed to express in seed by an endosperm specific promoter. Two steeping treatments, traditional steeping (TS, 0.2% SO2 + 0.5% lactic acid) and water steeping (WS, water only), were evaluated to determine effects on recovery of functional LT‐B in wet‐milled fractions. The overall recovery of the LT‐B protein from WS treatment was 1.5‐fold greater than that from TS treatment. In both steeping types, LT‐B was distributed similarly among the fractions, resulting in enrichment of functional LT‐B in fine fiber, coarse fiber and pericarp fractions by concentration factors of 1.5 to 8 relative to the whole kernels on a per‐mass basis. Combined with endosperm‐specific expression and secretory pathway targeting, wet‐milling enables enrichment of high‐value recombinant proteins in low‐value fractions, such as the fine fiber, and co‐utilization of remaining fractions in alternative industrial applications. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Development of multilayered cell‐hydrogel composites using an acoustic focusing technique
Authors: Mazzoccoli Jason P. , Feke Donald L. , Baskaran Harihara , Pintauro Peter N. 
Published: November 2009

AbstractMultilayered composites, composed of mammalian cells arranged in a hydrogel, have been prepared using an acoustic focusing technique. Acoustic focusing is a simple, nonchemical technique that allows for the fast arrangement of cells in matrices where the control of cell geometry is beneficial. Breast cancer cells (MDA‐MB231) were dispersed in a 30 wt % solution of poly(ethylene glycol) diacrylate (PEGDA) of molecular weight 400 at a density of 5 × 106 cells/mL of PEGDA solution. An ultrasonic field was used to organize the cells before polymerization of PEGDA. Disk‐shaped hydrogel composites, typically 1 cm in diameter and 2‐mm thick were prepared based on a PEGDA solution volume of 130 μL. At an acoustic frequency of 2.32 MHz, composites having cells positioned within concentric cylindrical shells interspersed with zones of cell‐free hydrogel were produced. The cells were located in annuli approximately 80‐μm thick and about 300 μm apart. The structure and viability of the cells within these constructs were studied using a fluorescent LIVE/DEAD assay. The viability of the cells was on the order of 50%. For the conditions used in this study, cell death was primarily attributed to exposure of cells to the PEGDA solution prior to polymerization, rather than adverse effects of polymerization or the sound field itself. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010
‘I'll sell this and I'll buy them that’: eBay and the management of possessions as stock
Authors: Denegri‐Knott Janice , Molesworth Mike 
Published: November 2009

Abstract

In this paper we document practices associated with selecting and selling previously owned goods through the online auction house and marketplace, eBay. More specifically, we discuss the processes through which the economic or exchange values of previously owned goods are re‐activated and the role eBay plays in facilitating such practices. Drawing from phenomenological interviews with heavy eBay users from various backgrounds living in the South of England, we discuss key emerging themes on the ways in which eBay is used for the disposal of goods. We find that eBay fuels practices of disposal that may encourage the transformation of the humble pre‐owned good into valuable stock. Besides those curative practices which have been captured in previous research into the divestment of possessions we find that work of another kind is required to move a used good back to a commodity phase in its career. We see this as turning used goods into stock. This transformation accelerates a good's biography as it enters the realm of the owned possession and then quickly returned to a sphere of exchange. In such process, goods become assets which are reinvested to fuel promiscuous consumer behaviours.

Copyright © 2009 John Wiley & Sons, Ltd.