bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Inter‐Model Uncertainty of Projected Precipitation Change in Northern China: The Modulating Role of North Atlantic Sea Surface Temperature
Authors: Wang Xin , Yu Haipeng , Wu Haojie , Cheng Shanling , Zhou Jie , Chen Siyu 
Published: June 2024

AbstractPrecipitation changes in northern China are projected to increase in the Coupled Model Inter‐comparison Project Phase 6 (CMIP6) multi‐model ensemble. However, these projections are accompanied by notable inter‐model uncertainty, and the sources of this uncertainty remain largely unexplored. By analyzing 30 CMIP6 models, this research explores the source of inter‐model uncertainty in projected precipitation change and reveals the fundamental mechanism driving uncertainty spread. Following the empirical orthogonal function of inter‐model projected precipitation change, the leading mode displays a seesaw spatial pattern between northwest and north China. This phenomenon predominantly stems from the inter‐model divergence of projected sea surface temperature (SST) warming in the North Atlantic. Further scrutinizing the ocean mixed layer heat budget, we discover that the combined effect of surface sensible heat flux, net surface shortwave radiation flux, and ocean heat transport convergence influences heat flux and SST change of North Atlantic. The multi‐model projections indicate that localized increases in solar radiation and heat convergence warm sea surface, raising SST and initiating convective motion. This convective motion subsequently transforms the 200 hPa teleconnection wave train, leading to an anti‐phase pattern over northern China. This wave pattern modulates total cloud cover percentage, influences surface upward latent heat flux, and adjusts the top of atmosphere outgoing longwave radiation, collectively resulting in the seesaw pattern. Our study underscores the pivotal role of inter‐model disparities in North Atlantic SST warming projection, which is a primary driver of precipitation uncertainty in northern China. These insights offer an essential foundation for refining and diminishing inter‐model uncertainty.
DPYD genetic polymorphisms in non-European patients with severe fluoropyrimidine-related toxicity: a systematic review
Authors: Chan Tsun Ho , Zhang J. Eunice , Pirmohamed Munir 
Published: June 2024

Abstract
Background
Pre-treatment DPYD screening is mandated in the UK and EU to reduce the risk of severe and potentially fatal fluoropyrimidine-related toxicity. Four DPYD gene variants which are more prominently found in Europeans are tested.

Methods
Our systematic review in patients of non-European ancestry followed PRISMA guidelines to identify relevant articles up to April 2023. Published in silico functional predictions and in vitro functional data were also extracted. We also undertook in silico prediction for all DPYD variants identified.

Results
In 32 studies, published between 1998 and 2022, 53 DPYD variants were evaluated in patients from 12 countries encompassing 5 ethnic groups: African American, East Asian, Latin American, Middle Eastern, and South Asian. One of the 4 common European DPYD variants, c.1905+1G>A, is also present in South Asian, East Asian and Middle Eastern patients with severe fluoropyrimidine-related toxicity. There seems to be relatively strong evidence for the c.557A>G variant, which is found in individuals of African ancestry, but is not currently included in the UK genotyping panel.

Conclusion
Extending UK pre-treatment DPYD screening to include variants that are present in some non-European ancestry groups will improve patient safety and reduce race and health inequalities in ethnically diverse societies.
Harnessing landrace diversity empowers wheat breeding
Authors: Cheng Shifeng , Feng Cong , Wingen Luzie U. , Cheng Hong , Riche Andrew B. , Jiang Mei , Leverington-Waite Michelle , Huang Zejian , Collier Sarah , Orford Simon , Wang Xiaoming , Awal Rajani , Barker Gary , O’Hara Tom , Lister Clare , Siluveru Ajay , Quiroz-Chávez Jesús , Ramírez-González Ricardo H. , Bryant Ruth , Berry Simon , Bansal Urmil , Bariana Harbans S. , Bennett Malcolm J. , Bicego Breno , Bilham Lorelei , Brown James K. M. , Burridge Amanda , Burt Chris , Buurman Milika , Castle March , Chartrain Laetitia , Chen Baizhi , Denbel Worku , Elkot Ahmed F. , Fenwick Paul , Feuerhelm David , Foulkes John , Gaju Oorbessy , Gauley Adam , Gaurav Kumar , Hafeez Amber N. , Han Ruirui , Horler Richard , Hou Junliang , Iqbal Muhammad S. , Kerton Matthew , Kondic-Spica Ankica , Kowalski Ania , Lage Jacob , Li Xiaolong , Liu Hongbing , Liu Shiyan , Lovegrove Alison , Ma Lingling , Mumford Cathy , Parmar Saroj , Philp Charlie , Playford Darryl , Przewieslik-Allen Alexandra M. , Sarfraz Zareen , Schafer David , Shewry Peter R. , Shi Yan , Slafer Gustavo A. , Song Baoxing , Song Bo , Steele David , Steuernagel Burkhard , Tailby Phillip , Tyrrell Simon , Waheed Abdul , Wamalwa Mercy N. , Wang Xingwei , Wei Yanping , Winfield Mark , Wu Shishi , Wu Yubing , Wulff Brande B. H. , Xian Wenfei , Xu Yawen , Xu Yunfeng , Yuan Quan , Zhang Xin , Edwards Keith J. , Dixon Laura , Nicholson Paul , Chayut Noam , Hawkesford Malcolm J. , Uauy Cristobal , Sanders Dale , Huang Sanwen , Griffiths Simon 
Published: June 2024

AbstractHarnessing genetic diversity in major staple crops through the development of new breeding capabilities is essential to ensure food security1. Here we examined the genetic and phenotypic diversity of the A. E. Watkins landrace collection2 of bread wheat (Triticum aestivum), a major global cereal, by whole-genome re-sequencing of 827 Watkins landraces and 208 modern cultivars and in-depth field evaluation spanning a decade. We found that modern cultivars are derived from two of the seven ancestral groups of wheat and maintain very long-range haplotype integrity. The remaining five groups represent untapped genetic sources, providing access to landrace-specific alleles and haplotypes for breeding. Linkage disequilibrium-based haplotypes and association genetics analyses link Watkins genomes to the thousands of identified high-resolution quantitative trait loci and significant marker–trait associations. Using these structured germplasm, genotyping and informatics resources, we revealed many Watkins-unique beneficial haplotypes that can confer superior traits in modern wheat. Furthermore, we assessed the phenotypic effects of 44,338 Watkins-unique haplotypes, introgressed from 143 prioritized quantitative trait loci in the context of modern cultivars, bridging the gap between landrace diversity and current breeding. This study establishes a framework for systematically utilizing genetic diversity in crop improvement to achieve sustainable food security.
Peripheral blood mononuclear cell respiratory function is associated with progressive glaucomatous vision loss
Authors: Petriti Bledi , Rabiolo Alessandro , Chau Kai-Yin , Williams Pete A. , Montesano Giovanni , Lascaratos Gerassimos , Garway-Heath David F. 
Published: June 2024

AbstractIntraocular pressure (IOP) is currently the only modifiable risk factor for glaucoma and all licensed treatments lower IOP. However, many patients continue to lose vision despite IOP-lowering treatment. Identifying biomarkers for progressive vision loss would have considerable clinical utility. We demonstrate that lower peripheral blood mononuclear cell (PBMC) oxygen consumption rate (OCR) is strongly associated with faster visual field (VF) progression in patients treated by lowering IOP (P < 0.001, 229 eyes of 139 participants), explaining 13% of variance in the rate of progression. In a separate reference cohort of untreated patients with glaucoma (213 eyes of 213 participants), IOP explained 16% of VF progression variance. OCR is lower in patients with glaucoma (n = 168) than in controls (n = 50; P < 0.001) and is lower in patients with low baseline IOP (n = 99) than those with high baseline IOP (n = 69; P < 0.01). PBMC nicotinamide adenine dinucleotide (NAD) levels are lower in patients with glaucoma (n = 29) compared to controls (n = 25; P < 0.001) and strongly associated with OCR (P < 0.001). Our results support PBMC OCR and NAD levels as new biomarkers for progressive glaucoma.
Complete genome of the Listeria monocytogenes strain AUF, used as a live listeriosis veterinary vaccine
Authors: Feodorova Valentina A. , Zaitsev Sergey S. , Khizhnyakova Mariya A. , Lavrukhin Maxim S. , Saltykov Yury V. , Zaberezhny Alexey D. , Larionova Olga S. 
Published: June 2024

AbstractListeria monocytogenes (Lm) is a highly pathogenic bacterium that can cause listeriosis, a relatively rare food-borne infectious disease that affects farm, domestic, wild animals and humans as well. The infected livestock is the frequent sources of Lm. Vaccination is one of the methods of controlling listeriosis in target farm animals to prevent Lm-associated food contamination. Here we report the complete sequence of the Lm strain AUF attenuated from a fully-virulent Lm strain by ultraviolet irradiation, successfully used since the 1960s as a live whole-cell veterinary vaccine. The de novo assembled genome consists of a circular chromosome of 2,942,932 bp length, including more than 2,800 CDSs, 17 pseudogenes, 5 antibiotic resistance genes, and 56/92 virulence genes. Two wild Lm strains, the EGD and the 10403S that is also used in cancer Immunotherapy, were the closest homologs for the Lm strain AUF. Although all three strains belonged to different sequence types (ST), namely ST12, ST85, and ST1538, they were placed in the same genetic lineage II, CC7.
Study of machine learning techniques for outcome assessment of leptospirosis patients
Authors: Silva Andreia Ferreira da , Figueiredo Karla , Falcão Igor W. S. , Costa Fernando A. R. , da Rocha Seruffo Marcos César , de Moraes Carla Cristina Guimarães 
Published: June 2024

AbstractLeptospirosis is a global disease that impacts people worldwide, particularly in humid and tropical regions, and is associated with significant socio-economic deficiencies. Its symptoms are often confused with other syndromes, which can compromise clinical diagnosis and the failure to carry out specific laboratory tests. In this respect, this paper presents a study of three algorithms (Decision Tree, Random Forest and Adaboost) for predicting the outcome (cure or death) of individuals with leptospirosis. Using the records contained in the government National System of Aggressions and Notification (SINAN, in portuguese) from 2007 to 2017, for the state of Pará, Brazil, where the temporal attributes of health care, symptoms (headache, vomiting, jaundice, calf pain) and clinical evolution (renal failure and respiratory changes) were used. In the performance evaluation of the selected models, it was observed that the Random Forest exhibited an accuracy of 90.81% for the training dataset, considering the attributes of experiment 8, and the Decision Tree presented an accuracy of 74.29 for the validation database. So, this result considers the best attributes pointed out by experiment 10: time first symptoms medical attention, time first symptoms ELISA sample collection, medical attention hospital admission time, headache, calf pain, vomiting, jaundice, renal insufficiency, and respiratory alterations. The contribution of this article is the confirmation that artificial intelligence, using the Decision Tree model algorithm, depicting the best choice as the final model to be used in future data for the prediction of human leptospirosis cases, helping in the diagnosis and course of the disease, aiming to avoid the evolution to death.
Comparison of juvenile and adult myasthenia gravis in a French cohort with focus on thymic histology
Authors: Truffault Frédérique , Auger Ludivine , Dragin Nadine , Vilquin Jean-Thomas , Fadel Elie , Thomas de Montpreville Vincent , Mansuet-Lupo Audrey , Regnard Jean-François , Alifano Marco , Sharshar Tarek , Behin Anthony , Eymard Bruno , Bolgert Francis , Demeret Sophie , Berrih-Aknin Sonia , Le Panse Rozen 
Published: June 2024

AbstractMyasthenia gravis (MG) is an autoimmune disease characterized by muscle fatigability due to acetylcholine receptor (AChR) autoantibodies. To better characterize juvenile MG (JMG), we analyzed 85 pre- and 132 post-pubescent JMG (with a cutoff age of 13) compared to 721 adult MG patients under 40 years old using a French database. Clinical data, anti-AChR antibody titers, thymectomy, and thymic histology were analyzed. The proportion of females was higher in each subgroup. No significant difference in the anti-AChR titers was observed. Interestingly, the proportion of AChR+ MG patients was notably lower among adult MG patients aged between 30 and 40 years, at 69.7%, compared to over 82.4% in the other subgroups. Thymic histological data were examined in patients who underwent thymectomy during the year of MG onset. Notably, in pre-JMG, the percentage of thymectomized patients was significantly lower (32.9% compared to more than 42.5% in other subgroups), and the delay to thymectomy was twice as long. We found a positive correlation between anti-AChR antibodies and germinal center grade across patient categories. Additionally, only females, particularly post-JMG patients, exhibited the highest rates of lymphofollicular hyperplasia (95% of cases) and germinal center grade. These findings reveal distinct patterns in JMG patients, particularly regarding thymic follicular hyperplasia, which appears to be exacerbated in females after puberty.
Dual-circulation: influence mechanism of ETS's carbon reduction and its spatiotemporal characteristics based on intensity modified SDID model
Authors: Tang Xinmeng , Qin Tao , He Xin , Kholaif Moustafa Mohamed Nazief Haggag Kotb 
Published: June 2024

AbstractTraditional DID models overlook variations in policy intensity, causing estimation deviations from the actual situation and a limited understanding of the influence mechanism. In response, the Intensity Modified SDID Model is built to examine the influence mechanism of ETS's carbon reductions. Moreover, through model extensions, the study explores the spatiotemporal characteristics and heterogeneities of ETS’s effects. Results show that: (1) "Dual-circulation" influence mechanism is confirmed, where ETS directly contributes to carbon reductions (2.70% to 10.0% impact) through external pathways, and internal pathways continuously strengthen reduction effects, comprehensive mechanisms are thereby formed and enhanced based on interaction among internal and external pathways. (2) Reasonable ETS levels are estimated and proposed to achieve "Dual Carbon Target", constraining nationwide carbon quotas by 20 billion tons/year, increasing carbon trading volumes by 80 thousand tons/year, and elevating the carbon trading prices by 100 RMB (14 USD) per ton. (3) ETS's carbon reduction effects are identified with temporal and spatial characteristics, temporally, effects peak in the 4th period (Event+4) but diminish in the 5th period (Event+5), spatially, effects peak in areas distancing around 1000 km but disappear beyond 1500 km. (4) ETS also has synergistic effects with atmospheric pollution reduction, including industrial emissions of sulfur dioxide and smoke (dust), but are insignificant to industrial emissions of wastewater and solid waste.
Effect of dietary Eucommia ulmoides oliver polysaccharide on immune function and meat quality of Songliao Black Pigs
Authors: Liang Yang , Tang Zeyu , Wang Hao , Liu Meng , Zhao Fanglin , Wang Longsheng , Meng Yinbiao , Jia Lijun 
Published: June 2024

AbstractEucommia ulmoides is a traditional Chinese herbal medicine, with pharmacological effects such as lowering blood pressure and enhancing immune function. The effects of dietary Eucommia ulmoides polysaccharide (EUP) on immune function and meat quality were studied in Songliao Black Pigs. Blood lymphocyte counts and percentage, concentrations of serum total protein and of albumin increased, whereas those of urea nitrogen and triglyceride decreased. White blood cell and lymphocyte counts, and serum IgA, IgE, IgG2 a and IFN-γ increased. Average daily weight gain, slaughter weight, lean meat rate and cooked meat rate increased, whereas pH24, feed-weight ratio, fat rate, yellowness (b#) and centrifugal dehydration rate decreased. Transcriptome sequencing of longissimus dorsi muscle detected 32 differentially expressed genes (DEGs), of which 26 were up-regulated and 6 down-regulated. A total of 19 genes were differentially expressed in the four groups, 18 of which were up-regulated. The DEGs included ADAMTS4, PER1, STAC, SERPINE1, FASN, THRSP, SP7 and KRT80 and the protein interaction network showed 20 up-regulated nodes, three down-regulated nodes and 14 DEGs. GO functional annotation and enrichment analysis showed that 34 items were significantly enriched, including transferase activity, actin binding, acetyl coenzyme A, acyl coenzyme A metabolism, adipose tissue development and acyl glycerol homeostasis. KEGG pathway analysis showed that the AMPK and PPAR signaling pathways were enriched. Dietary Eucommia polysaccharide enhanced immune function in Songliao Black Pigs, improved growth and carcass performance, increased the expression of genes related to meat quality traits and improved meat quality.
An explanatory study of factors influencing engagement in AI education at the K-12 Level: an extension of the classic TAM model
Authors: Li Wei , Zhang Xiaolin , Li Jing , Yang Xiao , Li Dong , Liu Yantong 
Published: June 2024

AbstractArtificial intelligence (AI) holds immense promise for K-12 education, yet understanding the factors influencing students’ engagement with AI courses remains a challenge. This study addresses this gap by extending the technology acceptance model (TAM) to incorporate cognitive factors such as AI intrinsic motivation (AIIM), AI readiness (AIRD), AI confidence (AICF), and AI anxiety (AIAX), alongside human–computer interaction (HCI) elements like user interface (UI), content (C), and learner-interface interactivity (LINT) in the context of using generative AI (GenAI) tools. By including these factors, an expanded model is presented to capture the complexity of student engagement with AI education. To validate the model, 210 Chinese students spanning grades K7 to K9 participated in a 1 month artificial intelligence course. Survey data and structural equation modeling reveal significant relationships between cognitive and HCI factors and perceived usefulness (PU) and ease of use (PEOU). Specifically, AIIM, AIRD, AICF, UI, C, and LINT positively influence PU and PEOU, while AIAX negatively affects both. Furthermore, PU and PEOU significantly predict students’ attitudes toward AI curriculum learning. These findings underscore the importance of considering cognitive and HCI factors in the design and implementation of AI education initiatives. By providing a theoretical foundation and practical insights, this study informs curriculum development and aids educational institutions and businesses in evaluating and optimizing AI4K12 curriculum design and implementation strategies.
Desensitizing efficacy of a universal dentin adhesive containing mesoporous bioactive glass on dentin hypersensitivity: a randomized clinical trial with a split-mouth model
Authors: Kim Hyun-Jung , Oh Soram , Kwon Jiyoung , Choi Kyoung-Kyu , Jang Ji-Hyun , Kim Duck-Su 
Published: June 2024

AbstractThis split-mouth blinded randomized controlled study compared the efficacy of a desensitizing agent with oxalate/resin polymer and a universal adhesive containing mesoporous bioactive glass (MBG) for dentin hypersensitivity (DH) relief, using Schiff sensitivity score (SSS) and visual analog scale (VAS). Split quadrants containing teeth with DH were treated with either MS Coat ONE or Hi-Bond Universal with MBG as the functional additive. Assessments at baseline, immediately post-application, and at 1- and 2-week follow-ups used standardized stimulus protocols (air, cold, and acid). The SSS difference was the primary outcome, while the VAS difference was the secondary outcome. A mixed linear effect model performed statistical analysis. Immediate DH reduction occurred in response to air stimuli, with a significant decrease in Group HB than in Group MS (p = 0.0178). Cold stimulus reduction exhibited a gradual cumulative effect, with consistently greater reductions in Group HB than in Group MS (p ≤ 0.0377). Both groups effectively managed acidic stimuli, with no significant differences (p > 0.05). The VAS scores decreased gradually over the follow-up period (p < 0.0001). This study highlights the differential efficacy of treatments for various DH triggers and recommends specific approaches based on different stimulus types. The universal adhesive containing MBG demonstrated DH relief potential, promising efficacy identical to or superior to that of a dedicated desensitizing agent. Further research exploring the long-term efficacy and underlying mechanisms is warranted. The universal adhesive containing MBG can be adopted as an in-office desensitizing agent for DH relief. The desensitizing efficacy of universal adhesive matches or surpasses dedicated agents for air and cold stimuli.
M133S mutation possibly involve in the ER stress and mitophagy pathway in maintenance hemodialysis patients with occult hepatitis B infection
Authors: Zou Yurong , Chen Sipei , Cui Yiyuan , Zou Yang 
Published: June 2024

AbstractOccult hepatitis B virus infection (OBI) is characterized by the presence of HBV DNA in the absence of detectable HBsAg. OBI is an important risk factor for cirrhosis and hepatocellular carcinoma, but its pathogenesis has not been fully elucidated. Mutations in the HBV preS/S genes can lead to impaired secretion of either HBsAg or S-protein resulting in the accumulation of defective viruses or S protein in cells. In our previous work, the M133S mutation was present in the HBV S gene of maintenance hemodialysis (MHD) patients with OBI. In this study, we investigated the potential role of amino acid substitutions in S proteins in S protein production and secretion through the construction of mutant S gene plasmids, structural prediction, transcriptome sequencing analysis, and in vitro functional studies. Protein structure prediction showed that the S protein M133S mutant exhibited hydrophilic modifications, with greater aggregation and accumulation of the entire structure within the membrane phospholipid bilayer. Differential gene enrichment analysis of transcriptome sequencing data showed that differentially expressed genes were mainly concentrated in protein processing in the endoplasmic reticulum (ER). The expression of heat shock family proteins and ER chaperone molecules was significantly increased in the wild-type and mutant groups, whereas the expression of mitochondria-associated proteins was decreased. Immunofluorescence staining and protein blotting showed that the endoplasmic reticulum-associated protein PDI, the autophagy marker LC3, and the lysosome-associated protein LAMP2 co-localized with the S proteins in the wild-type and mutant strains, and their expression was increased. The mitochondria-associated TOMM20 protein was also co-expressed with the S protein, but expression was significantly reduced in the mutant. The M133S mutation in the S gene is expressed as a defective and misfolded protein that accumulates in the endoplasmic reticulum causing secretion-impaired endoplasmic reticulum stress, which in turn triggers mitochondrial autophagy and recruits lysosomes to fuse with the autophagosome, leading to mitochondrial clearance. This study preliminarily demonstrated that the mutation of M133S in the S gene can cause OBI and is associated with disease progression, providing a theoretical basis for the diagnosis and treatment of OBI.