bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of dystrobrevin‐binding protein 1 (DTNBP1) on neural correlates of episodic memory encoding and retrieval
Authors: Thimm Markus , Krug Axel , Markov Valentin , Krach Sören , Jansen Andreas , Zerres Klaus , Eggermann Thomas , Stöcker Tony , Shah Nadim Jon , Nöthen Markus M. , Rietschel Marcella , Kircher Tilo 
Published: July 2009

AbstractEpisodic memory impairment is a frequently reported symptom in schizophrenia. It has been shown to be associated with reduced neural activity of the hippocampus and prefrontal cortex. Given the high heritability of schizophrenia the question arises if alterations in brain activity are modulated by susceptibility genes and might be detectable in healthy risk allele carriers. The present study investigated the effect of the single nucleotide polymorphism (SNP) rs1018381 (P1578) of the dystrobrevin‐binding protein 1 (DTNBP1) on brain activity in 84 healthy subjects assessed by functional magnetic resonance imaging (fMRI) while they performed an episodic memory task comprising encoding and retrieval of faces. During encoding, the group of risk allele carriers (n = 29) showed enhanced neural activity in the left middle frontal gyrus (BA 11) and bilaterally in the cuneus (BA 17, 7) when compared with the nonrisk carrier group (n = 55). During retrieval, the risk group (compared to the non risk group) showed increased right hemispheric neural activity comprising the medial frontal gyrus (BA 9), inferior frontal gyrus (BA 9), and inferior parietal lobule (BA 40). Since there were no behavioral performance differences, increased neural activity of the risk group might be interpreted as a correlate of higher effort or differing cognitive strategies in order to compensate for a genetically determined slight cognitive deficit. Interestingly, the laterality of increased prefrontal activity is in accordance with the well known hemispheric encoding/retrieval asymmetry (HERA) model of episodic memory. Hum Brain Mapp, 2010. © 2009 Wiley‐Liss, Inc.
Effect of 1α,25‐dihydroxyvitamin D3 in embryonic hippocampal cells
Authors: Marini Francesca , Bartoccini Elisa , Cascianelli Giacomo , Voccoli Vladimir , Baviglia Maria Gioia , Magni Mariapia Viola , Garcia‐Gil Mercedes , Albi Elisabetta 
Published: July 2009

AbstractAlthough the role of 1α,25‐dihydroxyvitamin D3 in calcium homeostasis of bone tissue is clear, evidence of the involvement of vitamin D3 in the central nervous system functions is increasing. In fact, vitamin D3 regulates vitamin D receptor and nerve growth factor expression, modulates brain development, and reverses experimental autoimmune encephalomyelitis. Only few studies, however, address vitamin D3 effect on embryonic hippocampal cell differentiation. In this investigation, the HN9.10e cell line was used as experimental model; these cells, that are a somatic fusion product of hippocampal cells from embryonic day‐18 C57BL/6 mice and N18TG2 neuroblastoma cells, show morphological and cytoskeletal features similar to their neuronal precursors. By this model, we have studied the time course of vitamin D3 localization in the nucleus and its effect on proteins involved in proliferation and/or differentiation. We found that the translocation of vitamin D3 from cytoplasm to the nucleus is transient, as the maximal nuclear concentration is reached after 10 h of incubation with 3H‐vitamin D3 and decreases to control values by 12 h. The appearance of differentiation markers such as Bcl2, NGF, STAT3, and the decrease of proliferation markers such as cyclin‐1 and PCNA are late events. Moreover, physiological concentrations of vitamin D3 delay cell proliferation and induce cell differentiation of embryonic cells characterized by modification of soma lengthening and formation of axons and dendrites. © 2009 Wiley‐Liss, Inc.
Cyclooxygenase‐2 inhibition inhibits PI3K/AKT kinase activity in epithelial ovarian cancer
Authors: Uddin Shahab , Ahmed Maqbool , Hussain Azhar , Assad Lina , Al‐Dayel Fouad , Bavi Prashant , Al‐Kuraya Khawla S. , Munkarah Adnan 
Published: July 2009

AbstractCyclooxygenase‐2 (COX‐2) expression contributes to tumor growth and invasion in epithelial ovarian cancer (EOC). COX‐2 inhibitors exhibit important anticarcinogenic potential against EOC, but the molecular mechanisms underlying this effect and relation with PI3‐kinase/AKT signaling remain the subject of intense investigations. Therefore, the role of COX‐2 in EOC and its cross talk with PI3‐kinase/AKT pathway were investigated using a large series of EOC tissues in a tissue micro array (TMA) format followed byin vitroandin vivostudies using EOC cell lines and NUDE mice. Clinically, COX‐2 was overexpressed in 60.3% of EOC and was significantly associated with activated AKT (p< 0.0001). Cox‐1 expression was seen in 59.9% but did not associate with AKT. Our in vitrodata using EOC cell line showed that inhibition of COX‐2 by aspirin, selective inhibitor NS398 and gene silencing by COX‐2 specific siRNA impaired phosphorylation of AKT resulting decreased downstream signaling leading to cell growth inhibition and induction of apoptosis. Finally, treatment of MDAH2774 cell line xenografts with aspirin resulted in growth inhibition of tumors in NUDE miceviadown‐regulation of COX‐2 and AKT activity. These data identify COX‐2 as a potential biomarker and therapeutic target in distinct molecular subtypes of ovarian cancer.
The bisphosphonate zoledronic acid has antimyeloma activityin vivoby inhibition of protein prenylation
Authors: Guenther Andreas , Gordon Sharon , Tiemann Markus , Burger Renate , Bakker Frank , Green Jonathan R. , Baum Wolfgang , Roelofs Anke J. , Rogers Michael J. , Gramatzki Martin 
Published: July 2009

AbstractNitrogen‐containing bisphosphonates (N‐BPs) are effective antiosteolytic agents in patients with multiple myeloma. Preclinical studies have also demonstrated that these agents have direct antitumor effectsin vitroand can reduce tumor burden in a variety of animal models, although it is not clear whether such effects are caused by direct actions on tumor cells or by inhibition of bone resorption. N‐BPs prevent bone destruction in myeloma by inhibiting the enzyme farnesyl pyrophosphate synthase in osteoclasts, thereby preventing the prenylation of small GTPase signaling proteins. In this study, utilizing a plasmacytoma xenograft model without complicating skeletal lesions, treatment with zoledronic acid (ZOL) led to significant prolongation of survival in severe combined immunodeficiency mice inoculated with human INA‐6 plasma cells. Following treatment with a clinically relevant dose of ZOL, histological analysis of INA‐6 tumors from the peritoneal cavity revealed extensive areas of apoptosis associated with poly (ADP‐ribose) polymerase cleavage. Furthermore, Western blot analysis of tumor homogenates demonstrated the accumulation of unprenylated Rap1A, indicative of the uptake of ZOL by nonskeletal tumors and inhibition of farnesyl pyrophosphate synthase. These studies provide, for the first time, clear evidence that N‐BPs have direct antitumor effects in plasma cell tumorsin vivoand this is executed by a molecular mechanism similar to that observed in osteoclasts.
A novel D‐glyceraldehyde‐3‐phosphate binding protein, a truncated albumin, with D‐glyceraldehyde‐3‐phosphate dehydrogenase inhibitory property
Authors: Roy Amrita , Bera Soumen , Patra Subrata , Ray Subhankar , Ray Manju 
Published: July 2009

AbstractWe have purified a novel protein from mice muscle, which through N‐terminal amino acid sequencing was identified as a truncated form of mouse albumin. The protein was found to be a monomer of ∼64 kDa and located in the cytosol. The purified protein strongly crossreacted with commercial albumin antibody. Presence of this protein was observed in different mouse organs. Further biochemical studies as well as CD spectroscopy indicated that the protein binds D‐glyceraldehyde‐3‐phosphate limiting the availability of the substrate to the enzyme D‐glyceraldehyde‐3‐phosphate dehydrogenase, thereby inhibiting its catalytic activity. The implication of this protein in the control of glycolysis has been discussed. © 2009 IUBMB IUBMB Life, 61(10): 995–1000, 2009
Synthesis and biological screening of some fluorinated dibenzofuran containing 3‐chlorochromones and benzothiazepines
Authors: Randhavane Pratibha , Karale Bhausaheb 
Published: July 2009

Abstract
magnified imageVariously substituted chalcones were synthesized from 4‐difluoromethoxy‐dibenzofuran‐1‐carboxaldehyde. These chalcones were converted into corresponding 3‐chlorochromones and dihydro‐benzothiazepines. Synthesized compounds were tested for their antifungal, antibacterial, antiviral and antioxidant activities. J. Heterocyclic Chem., (2009).
Microwave‐assisted multicomponent reaction for the synthesis of new and significative bisfunctional compounds containing two furo[3,4‐b]quinoline and acridinedione skeletons
Authors: Wang Xing‐Han , Hao Wen‐Juan , Tu Shu‐Jiang , Zhang Xiao‐Hong , Cao Xu‐Dong , Yan Shu , Wu Shan‐Shan , Han Zheng‐Guo , Shi Feng 
Published: July 2009

Abstract
magnified imageIn this study, a series of new and significative bisfunctional compounds containing two furo[3,4‐b]quinoline and acridinedione skeletons has been synthesized through a rapid one‐pot three‐component reaction of dialdehydes with N‐aryl enaminones and cyclic‐1,3‐dicarbonyl compounds (tetronic acid and cyclohexane‐1,3‐dione) in mixed solvent of glacial acetic acid and N,N‐dimethylformamide under microwave irradiation without catalyst. This method has the advantages of good yield and simple workup procedure. J. Heterocyclic Chem., (2009).
Primary cilia are highly oriented with respect to collagen direction and long axis of extensor tendon
Authors: Donnelly Eve , Ascenzi Maria‐Grazia , Farnum Cornelia 
Published: July 2009

AbstractSkeletal tissues adapt to their mechanical environments by modulating gene expression, cell metabolism, and extracellular matrix (ECM) architecture; however, the mechanosensory mechanisms for these processes are incompletely understood. Primary cilia have emerged as critical components of the cellular mechanosensory apparatus and have been hypothesized to participate in establishment of cellular and ECM orientation, but their function in skeletal tissues is just beginning to be examined. Here we focused on tendon, a tissue with an oriented matrix that is ideal for analysis of spatial relationships between primary cilia and the ECM. The objective of this study was to characterize the incidence and orientation of tenocyte primary cilia in their native ECM. Primary cilia, nuclei, and collagen were analyzed three‐dimensionally in immunofluorescently labeled rat extensor tendon using multiphoton microscopy and semiautomated morphometry. Primary cilia were observed in 64% of tenocytes. The cilia were highly oriented with respect to the ECM: cilia were aligned parallel to the collagen fibers and the long axis of the tendon. This study represents the first quantification of the in situ incidence and orientation of primary cilia in tendon. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:77–82, 2010
Osteogenesis induced by extracorporeal shockwave in treatment of delayed osteotendinous junction healing
Authors: Qin Ling , Wang Lin , Wan‐nar Wong Margaret , Wen Chunyi , Wang Gang , Zhang Ge , Chan Kai‐ming , Cheung Wing‐hoi , Leung Kwok‐sui 
Published: July 2009

AbstractHealing at the osteotendinous junction (OTJ) is challenging in orthopedic surgery. The present study aimed to test extracorporeal shockwave (ESW) in treatment of a delayed OTJ healing. Twenty‐eight rabbits were used for establishing a delayed healing (DH) model at patella‐patellar‐tendon (PPT) complex after partial patellectomy for 4 weeks and then were divided into DH and ESW groups. In the ESW group, a single ESW treatment was given at postoperative week 6 to the PPT healing complex. The samples were harvested at week 8 and 12 for radiographic and histological evaluations with seven samples for each group at each time point. Micro‐CT results showed that new bone volume was 1.18 ± 0.61 mm3 in the ESW group with no measurable new bone in the DH group at postoperative week 8. Scar tissue formed at the OTJ healing interface of the DH group, whereas ESW triggered high expression of VEGF in hypertrophic chondrocytes at week 8 and regeneration of the fibrocartilage zone at week 12 postoperatively. The accelerated osteogenesis could be explained by acceleration of endochondral ossification. In conclusion, ESW was able to induce osteogenesis at OTJ with delayed healing with enhanced endochondral ossification process and regeneration of fibrocartilage zone. These findings formed a scientific basis to potential clinical application of ESW for treatment of delayed OTJ healing. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:70–76, 2010
Versatility of polymethacrylate monoliths for chromatographic purification of biomolecules
Authors: Roberts Michael W. H. , Ongkudon Clarence M. , Forde Gareth M. , Danquah Michael K. 
Published: July 2009

AbstractPolymethacrylate monoliths, specifically poly(glycidyl methacrylate‐co‐ethylene dimethacrylate) or poly(GMA‐co‐EDMA) monoliths, are a new generation of chromatographic supports and are significantly different from conventional particle‐based adsorbents, membranes, and other monolithic supports for biomolecule purification. Similar to other monoliths, polymethacrylate monoliths possess large pores which allow convective flow of mobile phase and result in high flow rates at reduced pressure drop, unlike particulate supports. The simplicity of the adsorbent synthesis, pH resistance, and the ease and flexibility of tailoring their pore size to that of the target biomolecule are the key properties which differentiate polymethacrylate monoliths from other monoliths. Polymethacrylate monoliths are endowed with reactive epoxy groups for easy functionalization (with anion‐exchange, hydrophobic, and affinity ligands) and high ligand retention. In this review, the structure and performance of polymethacrylate monoliths for chromatographic purification of biomolecules are evaluated and compared to those of other supports. The development and use of polymethacrylate monoliths for research applications have grown rapidly in recent times and have enabled the achievement of high through‐put biomolecule purification on semi‐preparative and preparative scales.
Knowledge‐oriented process portal with BPM approach to leverage NPD management
Authors: Rozenfeld Henrique , Amaral Creusa Sayuri Tahara , da Costa Janaina Mascarenhas Hornos , Jubileu Andrea Padovan 
Published: July 2009

AbstractNew product development (NPD) processes can be improved through change projects managed in a context of business process management (BPM). In order to fully characterize and implement NPD process change projects it is necessary to be able to identify and adequately describe “to‐be” processes. These “to‐be” processes should be based on best practices, which can be structured into NPD reference models and body of knowledge (BOK). Despite the availability of NPD BOKs today, they are not easily applicable to process improvement, since they are published in on‐ and off‐line guidelines that are unsuitable for dynamic updating by a collaborative network such as a community of practice (CoP). A proposal is therefore put forward for the collaborative systematization of best practices through a two‐level portal external and internal to an organization, enabling users to participate in creating generic and public NPD reference models and BOKs. The proposal allows for the selection of a specific reference model and an existing BOK as required. This paper presents the main concepts underlying the proposal and its methodology, which focuses on agile project management (APM). It also describes the portal vision and the main deliverables of this research, with emphasis on the concept of the knowledge‐oriented process (KOP) portal. Three prototypes based on this concept are shown, which represent the evolutionary development of the KOP portal. Finally, the main results of the application are presented and discussed. Copyright © 2009 John Wiley & Sons, Ltd.
Real Space and Wave‐Number Space Studies of the Phase Structure and Morphology of iPP/PEOc Blends Using Scanning Electron Microscopy
Authors: Zhu Lin , Song Na , Xu Xinhua 
Published: July 2009

AbstractThe influence of blend composition on the phase structure and morphology of poly(propylene)/poly(ethylene‐co‐octene) blends was studied using SEM. A diameter dg was defined and calculated in real space to discuss the phase structure and morphology of iPP/PEOc blends. The figure‐estimation method was introduced to determine the distribution width of dg. It was shown that the distribution of dg obeys a log‐normal distribution and the distribution width σ of dg was calculated. In wave‐number (h) space, the correlation distance, ac, was defined by applying light scattering theory to power spectrum images obtained by 2D Fourier transformation. Moreover, a fractal dimension, Dc, was introduced to describe the uniformity of the spatial distribution.magnified image
In vitro digestion methods for assessing the effect of food structure on allergen breakdown
Authors: Wickham Martin , Faulks Richard , Mills Clare 
Published: July 2009

AbstractThis article reviews the in vitro digestion models developed to assess the stability of food allergens during digestion. It is hypothesised that food allergens must exhibit sufficient gastro‐intestinal stability to reach the intestinal mucosa where absorption and sensitisation (development of atopy) can occur. The investigation of stability of proteins within the gastrointestinal tract may provide prospective testing for allergenicity and could be a significant and valid parameter that distinguishes food allergens from nonallergens. Systematic evaluation of the stability of food allergens that are active via the gastrointestinal tract is currently tested in traditional pepsin digestibility models. The human gastrointestinal tract however is very complex and this article points out the importance of using physiologically relevant in vitro digestion systems for evaluating digestibility of allergens. This would involve the simulation of the stomach/small intestine environment (multi‐phase models) with sequential addition of digestive enzymes, surfactants such as phospholipids and bile salts under physiological conditions, as well as the consideration of the effect of the food matrices on the allergen digestion.
Utility of animal models for evaluating hypoallergenicity
Authors: Fritsché Rodolphe 
Published: July 2009

AbstractThe goal of this article is to describe the use of in vitro methods as well as in vivo and ex vivo animal models to assess residual allergenicity of hypoallergenic (HA) foods, especially infant formulas. These animal models are also used for testing the oral tolerance‐inducing capacities of infant formula; thus, they are very helpful in predicting allergenicity and the tolerogenic potential of HA infant formulas, and in helping to prevent adverse reactions in sensitive infants.
Protein hydrolysates from β‐conglycinin enriched soybean genotypes inhibit lipid accumulation and inflammation in vitro
Authors: Martinez‐Villaluenga Cristina , Dia Vermont P. , Berhow Mark , Bringe Neal A. , Gonzalez de Mejia Elvira 
Published: July 2009

AbstractObesity is a worldwide health concern and a well recognized predictor of premature mortality associated with a state of chronic inflammation. The objective was to evaluate the effect of soy protein hydrolysates (SPH) produced from different soybean genotypes by alcalase (SAH) or simulated gastrointestinal digestion (SGIH) on lipid accumulation in 3T3‐L1 adipocytes. The anti‐inflammatory effect of SPH produced by alcalase on LPS‐induced macrophage RAW 264.7 cell line was also investigated. SAH (100 μM) derived from soybean enriched in β‐conglycinin (BC) (up to 47% total protein) decreased lipid accumulation (33–37% inhibition) through downregulation of gene expression of lipoprotein lipase (LPL) and fatty acid synthase (FAS). SGIH (100 μM) inhibited lipid accumulation to a lesser extent (8–14% inhibition) through inhibition of LPL gene expression. SAH (5 μM) decreased the production of nitric oxide (NO) (18–35%) and prostaglandin E2 (PGE2) (47–71%) and the expression of inducible nitric oxide synthase (iNOS) (31–53%) and cycloxygenase‐2 (COX‐2) (30–52%). This is the first investigation showing that soy hydrolysates inhibit LPS‐induced iNOS/NO and COX‐2/PGE2 pathways in macrophages. Soybeans enriched in BCs can provide hydrolysates that limit fat accumulation in fat cells and inflammatory pathways in vitro and therefore warrant further studies as a healthful food.
Response to glucocorticoids and toxicity in childhood acute lymphoblastic leukemia: Role of polymorphisms of genes involved in glucocorticoid response
Authors: Marino Sara , Verzegnassi Federico , Tamaro Paolo , Stocco Gabriele , Bartoli Fiora , Decorti Giuliana , Rabusin Marco 
Published: July 2009

AbstractBackgroundGlucocorticoids (GCs) play a fundamental role in the treatment of pediatric acute lymphoblastic leukemia (ALL), but therapy with these agents often results in a number of severe side effects. The aim of our study was to evaluate the association between polymorphisms of genes encoding for proteins involved in the pharmacokinetics/pharmacodynamics of these drugs and the occurrence of side effects, in particular infections, in a small population of ALL children.ProcedureCommon polymorphisms of NR3C1, ABCB1, glutathione‐S‐transferase (GST)‐M1, GST‐P1, GST‐T1, and IL‐10 genes were analyzed in 36 pediatric patients with ALL, treated according to the AIEOP‐BMF ALL 2000 study protocol. Toxicities occurring during the induction and reinduction periods were assessed and their association with genotypes was evaluated.ResultsIn univariate analysis, the risk of severe infections was increased in subjects with the GST‐M1 null genotype, while patients with the GST‐M1 normal genotype had significantly more moderate degree infections. The results were confirmed by multivariate analysis. Selection from the reference models of independent variables based on Akaike Information Criteria (AIC) scores maintained the GST‐M1 genotype variable in the model to predict severe infections, and the ABCB1 C3435T and GST‐M1 genotype variables in the model for moderate infections.ConclusionsGST‐M1 genotype may influence the severity of infections in ALL children during GC administration. Pediatr Blood Cancer 2009;53:984–991. © 2009 Wiley‐Liss, Inc.