bluuebunny/crossref_metadata_2025_split huggingface dataset:
ChemInform Abstract: Microwave‐Assisted Palladium‐Catalyzed Heterogeneous Vinylation of 2(1H)‐Pyridones.
Authors: Singh Brajendra K. , Cavalluzzo Claudia , De Maeyer Marc , Debyser Zeger , Parmar Virinder S. , Van der Eycken Erik 
Published: January 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
Fluorescence Labeling and Determination of Pepsin with CdSe Quantum Dots
Authors: Chen Lihua , Zhang Wenlong , Jiao Peng , Zhao Mingming 
Published: January 2010

AbstractBased on the solid phase/liquid deposition CdSe quantum dots (QD) were synthesized using selenium and cadmium‐salt as precursor at room temperature. The average diameter of CdSe QD estimated from the high resolution transmission electron microscopy (HRTEM) graph and absorption spectra was ca. 3–3.5 nm. The mercaptoacetic‐acid stabilized CdSe QD exhibited ultraviolet absorption at 350 and 380 nm and strong fluorescence emission at 481.6 nm, respectively. When conjugated with pepsin, the fluorescence peak intensity of CdSe QD decreased considerably and the fluorescence peak shifted to 467.2 nm. Under optimal conditions, a concentration in 5–50 mg· L−1 of pepsin could be determined on the basis of fluorescence decrease ratio of CdSe QD, with a detection limit 3δ of 0.36 mg·L−1 (n=10). The proposed method was applied to detection of the concentration of pepsin in human gastric juice.
Fabrication of Bismuth/Multi‐walled Carbon Nanotube Composite Modified Glassy Carbon Electrode for Determination of Cobalt
Authors: Wang Yuane , Pan Dawei , Li Xinming , Qin Wei 
Published: January 2010

AbstractA bismuth/multi‐walled carbon nanotube (Bi/MWNT) composite modified electrode for determination of cobalt by differential pulse adsorptive cathodic stripping voltammetry is described. The electrode is fabricated by potentiostatic pre‐plating bismuth film on an MWNT modified glassy carbon (GC) electrode. The Bi/MWNT composite modified electrode exhibits enhanced sensitivity for cobalt detection as compared with the bare GC, MWNT modified and bismuth film electrodes. Numerous key experimental parameters have been examined for optimum analytical performance of the proposed electrode. With an adsorptive accumulation of the Co(II)‐dimethylglyoxime complex at −0.8 V for 200 s, the reduction peak current is proportional to the concentration of cobalt in the range of 4.0×10−10−1.0×10−7 mol/L with a lower detection limit of 8.1×10−11 mol/L. The proposed method has been applied successfully to cobalt determination in seawater and lake water samples.
A Stable Isotopic Pre‐digestion Labeling Method for Protein Quantitative Analysis Using Matrix‐assisted Laser Desorption/ionization Mass Spectrometry
Authors: Fang Fang , Zhang Jing , Zhang Li , Guo Yinlong 
Published: January 2010

AbstractAs an extension of our previous work, here a strategy was demonstrated for protein identification and quantification analyses utilizing a combination of stable isotope chemical labeling with subsequent denaturation, enzymatic digestion and matrix assisted laser desorption/ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS). Using [d0]‐ and [d6]‐4,6‐dimethoxy‐2‐(methylsulfonyl)pyrimidine ([d0]‐/[d6]‐DMMSP), stable isotopic labels were incorporated before digestion. The comparative samples were combined before labeling after digestion, thus biases resulting from differences in sample digestion were avoided and the higher accuracy of quantification could be attained. The labeling was spatial‐selective to particular residues of cysteine, lysine, and tyrosine before denaturation, which could lead to a better universality of the strategy for cysteine‐free proteins. In addition, some lysine residues were blocked after labeling, the partly destroyed recognition sites could simplify the trypsin hydrolysates and hence facilitate the MS complexity. Together, our one‐step labeling strategy combined several desirable properties such as spatial‐selective labeling, reliability of quantitative results, simplification of analysis of complex systems and direct analysis with minimum sample handling. Our results demonstrate the usefulness of the method for analyzing lysozyme in egg white. The method was expected to provide a new powerful tool for comparative proteome research.
Atmospheric Reactions of (H)‐ and (D)‐Fluoroalcohols with Chlorine Atoms
Authors: Garzón Andrés , Moral Mónica , Notario Alberto , Ceacero‐Vega Antonio A. , Fernández‐Gómez Manuel , Albaladejo José 
Published: January 2010

AbstractThe reactions of Cl with a series of fluoroalcohols and deuterated fluoroalcohols, CF3CH2OH (k4), CF3CH(OH)CH3 (k5), CF3CH(OH)CF3 (k6), CF3CH(OD)CF3 (k7) and CF3CD(OD)CF3 (k8), are investigated as a function of temperature in the range of 268–378 K by laser photolysis–resonance fluorescence. To our knowledge, only the CF3CH2OH+Cl reaction has been previously studied from a kinetic point of view. The derived Arrhenius expressions obtained using our kinetic data are: k4=(1.79±0.17)×10−13 exp[(410±26)/T], k5=(1.20±0.11)×10−12 exp[(394±14)/T], k6=(2.32±0.18)×10−13 exp[−(740±12)/T], k7=(6.45±1.87)×10−13 exp[−(1136±94)/T] and k8=(4.19±1.09)×10−13 exp[−(1378±81)/T] (in units of cm3 molecule−1 s−1 and where errors are ±σ). Moreover, a theoretical insight into the mechanisms of these reactions is pursued through ab initio Möller–Plesset second‐order perturbation treatment calculations with the 6‐311G** basis set. Optimized geometries are obtained for reagents, transition states and molecular complexes appearing along the different reaction pathways. Furthermore, molecular energies are calculated at the quadratic configuration interaction with single, double and triple excitations [QCISD(T)] level to obtain an estimation of the activation energies. Finally, the rate constants are calculated through transition‐state theory using Wigner’s transmission coefficient in order to include the tunnelling‐effect corrections.
Excited‐State Dynamics of the Metal String Complex Co3(dpa)4(NCS)2 from Femtosecond Transient Absorption Spectra
Authors: Cheng Chao‐Han , Hung Ruei‐Ding , Wang Wen‐Zhen , Peng Shie‐Ming , Chen I‐Chia 
Published: January 2010

AbstractTransient absorption spectroscopy is used to study the excited‐state dynamics of Co3(dpa)4(NCS)2, where dpa is the ligand di(2‐pyridyl)amido. The ππ*, charge‐transfer, and d–d transition states are excited upon irradiation at wavelengths of 330, 400 and 600 nm, respectively. Similar transient spectra are observed under the experimental temporal resolution and the transient species show weak absorption. We thus propose that a low‐lying metal‐centered d–d state is accessed immediately after excitation. Analyses of the experimental kinetic traces reveal rapid conversion from the ligand‐centered ππ* and the charge‐transfer states to this metal‐centered d‐d state within 100 fs. The excited molecule then crosses to a second d–d state within the ligand‐field manifold, with a time coefficient of 0.6–1.4 ps. Because the ground‐state bleaching band recovers with a time coefficient of 10–23 ps, we propose that an excited molecule crosses from the low‐lying d–d state either directly within the same spin system or with spin crossing via the state 2B to the ground state 2A2 (symmetry group C4). In this trimetal string complex, relaxation to the ground electronic surface after excitation is thus rapid.
Reductive Splitting of Cellulose in the Ionic Liquid 1‐Butyl‐3‐Methylimidazolium Chloride
Authors: Ignatyev Igor A. , Van Doorslaer Charlie , Mertens Pascal G. N. , Binnemans Koen , De Vos Dirk E. 
Published: January 2010

AbstractThe depolymerization of cellulose is carried out in the ionic liquid 1‐butyl‐3‐methylimidazolium chloride in the presence of hydrogen gas. First, the ketal 1,1‐diethoxycyclohexane and cellobiose were used as model substrates. For the depolymerization of cellulose itself, the combination of a heterogeneous metal catalyst and a homogeneous ruthenium catalyst proved effective. One of the possible roles of the ruthenium compound is to enhance the transfer of hydrogen to the metallic surface. The cellulose is fully converted under relatively mild conditions, with sorbitol as the dominant product in 51–74 % yield.
The Presence of Functional Groups Key for Biodegradation in Ionic Liquids: Effect on Gas Solubility
Authors: Deng Yun , Morrissey Saibh , Gathergood Nicholas , Delort Anne‐Marie , Husson Pascale , Costa Gomes Margarida F. 
Published: January 2010

AbstractThe effect of the incorporation of either ester or ester and ether functions into the side chain of an 1‐alkyl‐3‐methylimidazolium cation on the physico‐chemical properties of ionic liquids containing bis(trifluoromethylsulfonyl)imide or octylsulfate anions is studied. It is believed that the introduction of an ester function into the cation of the ionic liquids greatly increases their biodegradability. The density of three such ionic liquids is measured as a function of temperature, and the solubility of four gases—carbon dioxide, ethane, methane, and hydrogen—is determined between 303 K and 343 K and at pressures close to atmospheric level. Carbon dioxide is the most soluble gas, followed by ethane and methane; the mole fraction solubilities vary from 1.8×10−3 to 3.7×10−2. These solubilities are of the same order of magnitude as those determined for alkylimidazolium‐based ionic liquids. The chemical modification of the alkyl side chain does not result in a significant change of the solvation properties of the ionic liquid. All of the solubilities decrease with increasing temperature, corresponding to an exothermal solvation process. From the variation of this property with temperature, the thermodynamic functions of solvation (Gibbs energy, enthalpy, and entropy) are calculated and provide information about the solute–solvent interactions and the molecular structure of the solutions.
Reed‐sternberg cells in breast FNA of a patient with left breast mass
Authors: Park Jaemin , Rizzo Monica , Jackson Simona , Bates Sandra R. , Green Victoria , Oprea‐Ilies Gabriela 
Published: January 2010

AbstractHodgkin's lymphoma is a potentially curable malignancy of the lymphatic system characterized by a variable number of scattered and large mononucleated and multinucleated tumor cells, the Hodgkin and Reed‐Sternberg cells residing in an abundant heterogeneous admixture of non‐neoplastic inflammatory cells. It represents approximately 30% of all lymphomas according to the World Health Organization (WHO). Patients with Hodgkin's lymphoma typically present with painless peripheral adenopathy, fever, night sweats, and weight loss. We report a rare case of Hodgkin's lymphoma presented as a breast mass in a 23‐year‐old woman diagnosed on fine needle aspiration (FNA). At presentation, she had no B symptoms, or palpable lymphadenopathy. Diagn. Cytopathol. 2010;38:663–668. © 2010 Wiley‐Liss, Inc.
Micronucleus in atypical urothelial cells
Authors: Arora Sandeep Kumar , Dey Pranab , Saikia Uma Nahar 
Published: January 2010

AbstractThe objective of this study is to evaluate the role of micronuclei (MN) in identifying the atypical urothelial cells urinary cytology. This is a retrospective study of a total of 30 cases of urine samples. Of these, 15 cases were selected where the initial diagnosis was atypical urothelial cell and later turned out as malignant cells on follow‐up histopathology. The rest of the 15 cases were reported as normal urothelial cells. The number of MN in 1,000 urothelial cells was counted in all the cases and was expressed as percentage. All the cases diagnosed as atypical urothelial cells show the presence of MN. The mean number of MN was 2.53 ± 0.99%. No MN was noted in any of the normal urothelial cells in control cases. The Student's t‐test of MN scoring was highly significant (P < 0.000) in test (atypical urothelial cells) versus control group (benign urothelial cells). MN is an important biomarker of malignancy. Micronuclear score in atypical urothelial cells in urine cytology smears may help in proper diagnosis of the case. Diagn. Cytopathol. 2010;38:811‐813. © 2010 Wiley‐Liss, Inc.
Metastatic peritoneal mesothelioma in the setting of recurrent ascites: A case report
Authors: Samedi Von , White Sherrie , Zimarowski Mary Jane , Harris Anna , Saffitz Jeffrey , Wang Helen H. 
Published: January 2010

AbstractMalignant peritoneal mesothelioma is uncommon but rapidly fatal with a median survival of less than 1 year. The diagnosis of this entity is often delayed because of the nonspecific presenting symptoms and nonspecific cytological features of the mesothelial cells in the peritoneal fluids.A 72‐year‐old man who had no known history of exposure to asbestos and had longstanding refractory ascites thought to be secondary to alcoholic cirrhosis was found to have widespread metastatic malignant mesothelioma involving the lung, liver, pancreas, peritoneal, and pelvic wall, skin and subcutaneous tissue. Diagn. Cytopathol. 2010;38:675–681. © 2009 Wiley‐Liss, Inc.
KRAS mutation analysis on cytological specimens of metastatic colo‐rectal cancer
Authors: Troncone Giancarlo , Malapelle Umberto , Cozzolino Immacolata , Palombini Lucio 
Published: January 2010

AbstractRecent evidences showed that metastatic colorectal cancer (CRC) patients with tumors harboring a KRAS gene mutation do not derive benefit from the administration of epidermal growth factor receptor‐directed monoclonal antibodies. Typically, the specimens available for KRAS mutational analysis are formalin‐fixed paraffin‐embedded (FFPE) primary tumor tissue blocks. However, in patients with rectal tumours undergoing neoadjuvant therapy, the source of FFPE material is limited. In this setting, CRC cytological samples taken from the metastatic site may be exploited. However, these specimens show at least some degree of necrosis; thus, their suitability for the KRAS assay needs to be tested. Here, we show that 18/19 (94.7%) metastatic CRC smears were perfectly adequate for codon 12 and 13 KRAS mutational analysis by direct gene sequencing. Only one case (5.3%) showing abundant necrotic debris and poor cellular preservation was not informative for KRAS status. Codon 12 gene mutations were found in 4/18 (22.2%) of the adequate cases (c35G>T n = 2; c34G>T n = 1; c35G>A n = 1). Concordance between cytological and FFPE samples, both available in 13 patients, occurred in 92.3% (12/13) of the cases. Thus, whenever histological specimens of CRC are notavailable, KRAS testing may be reliably performed on cytological specimens. Diagn. Cytopathol. 2010;38:869–873. © 2010 Wiley‐Liss, Inc.
The sensitivity of cytologic evaluation of pleural fluid in the diagnosis of malignant mesothelioma
Authors: Rakha E. A. , Patil S. , Abdulla K. , Abdulkader M. , Chaudry Z. , Soomro I. N. 
Published: January 2010

AbstractPleural malignant mesothelioma (MM), which is an aggressive neoplasm with a high mortality, frequently manifests initially as pleural effusions. The sensitivity of cytologic examination for its diagnosis varies widely in literature and most of the figures are from earlier studies with conventional cytologic preparations. The objective of this study was to provide the current evidence on the role and sensitivity of cytologic examination of pleural fluid in the diagnosis of MM. We reviewed the cytologic findings in pleural effusions of a large series of histologically proven MM (234 cases) diagnosed in our institution between 2001 and 2008. Of all cases, 154 (66%) had cytologic material examined. A specific diagnosis of MM was rendered or suspected in 53% (79 patients). The lowest sensitivity (20%) was noticed in sarcomatoid MM cases. MM was favored over adenocarcinoma in 97% of patients with positive cytologic findings that have been confirmed with immunohistochemistry. In this series, five cases were inadequate and five cases were initially reported as atypical, whereas 65 cases (44%) were reported as negative for malignancy. On review of the cytology slides, only four cases were upgraded from benign to suspicious compared to four cases downgraded from suspicious to atypical but no significant improvement to the diagnosis could be made on revision. These data suggested that a cytologic diagnosis contributed useful information in patients with epithelioid and biphasic pleural MM. Limitations of the cytologic examination of MM should also be acknowledged. Diagn. Cytopathol. 2010;38:874–879. © 2010 Wiley‐Liss, Inc.
Regulation of high‐density lipoprotein by inflammatory cytokines: establishing links between immune dysfunction and cardiovascular disease
Authors: Haas Michael J. , Mooradian Arshag D. 
Published: January 2010

AbstractCoronary artery disease is a primary co‐morbidity in metabolic diseases such as metabolic syndrome, diabetes and obesity. One contributing risk factor for coronary artery disease is low high‐density lipoprotein‐cholesterol (HDLc). Several factors influence steady‐state HDLc levels, including diet, genetics and environment. Perhaps more important to coronary artery disease is factors that attribute to the dynamics of reverse cholesterol transport, storage, and excretion of excess cholesterol. HDLc biogenesis, clearance and innate ability to serve as a cholesterol acceptor and transporter all contribute to HDLc's function as a negative regulator of cardiovascular disease. With the recent failure of torcetrapid, focus is being placed on HDLc biology and its role in various metabolic diseases. Low HDLc levels are often associated with an increased state of background inflammation. Recently, several syndromes with clear pro‐inflammatory components have been shown to be inversely correlated with low HDLc levels in the absence of obesity, diabetes and metabolic syndrome. Early studies with HDLc during the acute‐phase response suggest that HDLc is substantially physically modified during acute infection and sepsis, and recent studies show that HDLc is physically modified by chronic pro‐inflammatory disease. In this review, several of these connections are described and cytokine signalling related to HDLc is examined. Copyright © 2010 John Wiley & Sons, Ltd.
Detection of mono‐hydroxylated metabolites of stanozolol by HPLC‐ESI (+) MS/MS in Indian sports persons
Authors: Ahi S. , Beotra A. , Jain S. 
Published: January 2010

AbstractThe abuse of stanozolol is quite widespread in Indian sport. Its analysis is challenging and this has led to the development of new methods to improve its detection. A method was developed and validated for the detection of the three main monohydroxylated metabolites of stanozolol. The excretion profile of these metabolites was studied in four healthy male volunteers. The excretion study samples, after a single oral dose of drug, showed that 3′‐OH‐stanozolol was excreted at the highest concentration, followed by 16β‐OH stanozolol, with 4β‐OH stanozolol as the least excreted. Ninety‐eight old doping samples with adverse analytical findings for 3′‐OH‐stanozolol were reanalysed using this method. This showed 3′‐OH‐stanozolol and 16β‐OH stanozolol in all the 98 samples whereas 4β‐OH‐stanozolol was identified in 90 samples. The percentage of positive identifications of stanozolol in Indian sportspeople has increased markedly in the last five years, from 31.9% in 2004 to 81.8% in 2009; however, this may be due to the more effective detection of stanozolol metabolites. It can thus be concluded that the marked increase in percent positive of stanozolol in Indian sportspersons in 2009 may be due to the improved detection by a more effective LCMS/MS method. Copyright © 2010 John Wiley & Sons, Ltd.
T‐bet, a Th1 transcription factor regulates the expression of Tim‐3
Authors: Anderson Ana C. , Lord Graham M. , Dardalhon Valerie , Lee David H. , Sabatos‐Peyton Catherine A. , Glimcher Laurie H. , Kuchroo Vijay K. 
Published: January 2010

AbstractT‐cell immunoglobulin, mucin domain‐3 (Tim‐3) is a membrane protein expressed at late stages of IFN‐γ secreting CD4+ Th1 cell differentiation and constitutively on DC. Ligation of Tim‐3 on Th1 cells terminates Th1 immune responses. In addition, Tim‐3 plays a role in tolerance induction, although the mechanism by which this is accomplished has yet to be elucidated. While it is clear that Tim‐3 plays an important role in the immune system, little is known regarding the molecular pathways that regulate Tim‐3 expression. In the current study, we examine the role of Th1‐associated transcription factors in regulating Tim‐3 expression. Our experiments reveal that Tim‐3 expression is regulated by the Th1‐specific transcription factor T‐bet. This introduces a novel paradigm into the generation of a Th1 response, whereby a transcription factor responsible for effector Th1 cell differentiation also increases the expression of a specific counter‐regulatory molecule to ensure appropriate termination of pro‐inflammatory Th1 immune responses.
c‐Rel is crucial for the induction of Foxp3+ regulatory CD4+ T cells but not TH17 cells
Authors: Visekruna Alexander , Huber Magdalena , Hellhund Anne , Bothur Evita , Reinhard Katharina , Bollig Nadine , Schmidt Nicole , Joeris Thorsten , Lohoff Michael , Steinhoff Ulrich 
Published: January 2010

AbstractThe NF‐κB/Rel family member c‐Rel was described to be required for the development of TH1 responses. However, the role of c‐Rel in the differentiation of TH17 and regulatory CD4+Foxp3+ T cells (Treg) remains obscure. Here, we show that in the absence of c‐Rel, in vitro differentiation of pro‐inflammatory TH17 cells is normal. In contrast, generation of inducible Treg (iTreg) within c‐Rel‐deficient CD4+ T cells was severely hampered and correlated to reduced numbers of Foxp3+ T cells in vivo. Mechanistically, in vitro conversion of naive CD4+ T cells into iTreg was crucially dependent on c‐Rel‐mediated synthesis of endogenous IL‐2. The addition of exogenous IL‐2 was sufficient to rescue the development of c‐Rel‐deficient iTreg. Thus, c‐Rel is essential for the development of Foxp3+ Treg but not for TH17 cells via regulating the production of IL‐2.
Synthesis, Crystal Structure, and Catalytic Properties of Novel Dioxidomolybdenum(VI) Complexes with Tridentate Schiff Base Ligands in the Biomimetic and Highly Selective Oxygenation of Alkenes and Sulfides
Authors: Rezaeifard Abdolreza , Sheikhshoaie Iran , Monadi Niaz , Stoeckli‐Evans Helen 
Published: January 2010

AbstractFour novel dioxidomolybdenum(VI) complexes [MoO2(Lx)(CH3OH)] have been synthesized, using 2[(E)‐(2‐hydroxy‐2‐phenylethylimino)methyl]phenol derivatives as tridentate ONO donor Schiff base ligands (H2Lx) and MoO2(acac)2. A monoclinic space group was determined by X‐ray crystallography from single‐crystal data of a sample of these new complexes. The epoxidation of alkenes by using tert‐butyl hydroperoxide and oxidation of sulfides to sulfoxides by urea hydrogen peroxide were efficiently enhanced with excellent selectivity under the catalytic influence these new MoVI complexes. The high efficiency and relative stability of the catalysts have been observed by turnover numbers and UV/Vis investigations. The electron‐poor and bulky ligands promoted the effectiveness of the catalysts.
On the Spin Behaviour of Iron(II)–Dipyridyltriazine Complexes and Their Performance as Thermal and Photonic Spin Switches (Eur. J. Inorg. Chem. 2/2010)
Authors: Hain Sophie K. , Heinemann Frank W. , Gieb Klaus , Müller Paul , Hörner Gerald , Grohmann Andreas 
Published: January 2010

AbstractThe cover picture shows the crystal structure of an iron(II) complex with a tridentate triazine‐based ligand, one of three complexes whose thermal and photonic spin‐dynamics are discussed in the article by G. Hörner, A. Grohmann et al. on p. 221 ff. While there is a competition between spin crossover and ligand exchange upon thermal excitation in solution, the complexes switch cleanly between their stable low‐spin and metastable high‐spin forms upon photoexcitation. Artwork: Holger Neumann, Gentura.