bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transforming growth factor‐beta‐induced protein secreted by peritoneal cells increases the metastatic potential of ovarian cancer cells
Authors: Ween Miranda P. , Lokman Noor A. , Hoffmann Peter , Rodgers Raymond J. , Ricciardelli Carmela , Oehler Martin K. 
Published: June 2010

AbstractOvarian cancer metastasis is characterized by the shedding of malignant cells from the surface of the ovary and their implantation onto the peritoneal surface, which lines the abdominal cavity. As the factors promoting this process are poorly understood, we investigated the ovarian cancer–peritoneal interaction by means of in vitro coculture experiments with ovarian cancer (OVCAR‐5 and SKOV‐3) and peritoneal (LP‐9) cells. One of the proteins differentially expressed in the coculture secretome was identified by MALDI‐TOF/TOF mass spectrometry as the extracellular matrix protein transforming growth factor‐beta‐induced protein (TGFBIp, also known as βig‐H3). Immunohistochemistry showed high TGFBIp levels in normal surface ovarian epithelial and peritoneal cells, whereas TGFBIp levels in primary serous ovarian carcinomas and matching metastatic implants was very low. In functional in vitro experiments, treatment with recombinant TGFBIp significantly increased the motility and invasiveness of OVCAR‐5 and SKOV‐3 cells and significantly increased ovarian cancer cell (OVCAR‐5, OVCAR‐3 and SKOV‐3) adhesion to LP‐9 cells. TGFBIp was found to be processed at both the N‐ and C‐terminus in the secretome of the ovarian cancer–peritoneal cell coculture. Plasmin inhibitors blocked TGFBIp processing and significantly reduced OVCAR‐5 cell adhesion to peritoneal cells. We conclude that TGFBIp expressed by peritoneal cells increases the metastatic potential of ovarian cancer cells. TGFBIp is therefore a potential novel therapeutic target against ovarian cancer.
Rural and Urban Streams: Anthropogenic Influences and Impacts on Water and Sediment Quality
Authors: Kühl Adelina Maria , Mello Sartori Cardoso da Rocha Carmen Lúcia , Gaeta Espíndola Evaldo Luiz , Lansac‐Tôha Fábio Amodêo 
Published: June 2010

AbstractThe Pirapó river watershed (Paraná State, Brazil) compounds a relatively industrialized and urbanized region, undergoing great pressure from the discharge of industrial, agricultural and domestic wastes. We evaluated the environmental quality of ten streams belonging to this watershed in April and June 2008 by performing acute and chronic toxicity tests with Daphnia similis and Ceriodaphnia silvestrii from water and sediment samples. We tested the hypothesis that the streams located in urban areas are more exposed to the influence of pollutants, than those outside the city limits. In addition, we obtained the measures of physical and chemical parameters, and identified the main polluted sources. Contrary to what was expected, the rural streams were more toxic than those located in urban area. These results demonstrate that the water bodies located in rural areas are being affected by the pollution of aquatic ecosystems as far as those found in urban areas, requiring the same attention of environmental managers in relation to its monitoring. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Differential Responses of Epiphytic and Planktonic Toxic Cyanobacteria to Allelopathic Substances of the Submerged Macrophyte Stratiotes aloides
Authors: Mohamed Zakaria A. , Al Shehri Abdulrahman M. 
Published: June 2010

AbstractThe present study investigates the allelopathic effects of Stratiotes aloides on the growth and antioxidative biomarkers of epiphytic and planktonic toxic cyanobacteria in a batch experiment over a 15‐day exposure to different concentrations of aqueous acetone extract of this macrophyte. The results showed that epiphytic and planktonic species of cyanobacteria showed differential responses to the allelopathic activity of this macrophyte. S. aloides extract reduced the growth, alkaline phosphatase activity (APA) and toxin production of the planktonic cyanobacterium, Anabaena variabilis, with an increase in lipid peroxidation, glutathione and activities of antioxidative enzymes of this cyanobacterium. Conversely, this extract did not affect the growth and increased the toxin production in epiphytic cyanobacteria, with no significant effect on lipid peroixdation or the activities of APA and antioxidative enzymes of these species. This study is the first to suggest that the macrophyte S. aloides supports the growth and toxin production of its epiphytic cyanobacteria. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
The Environment of Lake Victoria (East Africa): Current Status and Historical Changes
Authors: Sitoki Lewis , Gichuki John , Ezekiel Charles , Wanda Fred , Mkumbo Oliva C. , Marshall Brian E. 
Published: June 2010

AbstractBeginning in the mid‐1980s Lake Victoria experienced severe eutrophication and it was suggested that deteriorating water quality might lead to a collapse of its fisheries. A series of lake‐wide surveys carried out 1999–2001 and 2005–2009 revealed that the temperature of the lake had risen by > 1 °C since 1927, with more rapid warming of the deeper waters reducing the thermal gradient in the water column and thus weakening stratification and the extent and severity of deoxygenation. The chlorophyll a concentrations in open water decreased since the 1980s, while Secchi disc visibility increased, indicating a reduced severity of algal blooms. Chlorophyll a was higher and Secchi disc visibility lower in inshore waters but there has been no deterioration in these areas since the 1980s. The conductivity remained unchanged, although it was about 50% greater in the semi‐enclosed Nyanza Gulf than in the open lake. The water quality of the lake has therefore improved considerably despite the fact than concentrations of plant nutrients have not decreased and the reasons why this may be the case are discussed. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
A new amido phosphonate derivative of carboxymethylcellulose with an osteogenic activity and which is capable of interacting with any Ti surface
Authors: Barbucci Rolando , Arturoni Enrico , Panariello Giuseppe , Di Canio Clara 
Published: June 2010

AbstractA new phosphonate derivative of carboxymethylcellulose (CMC) was recently synthesized (CMCAPh). The phosphonate polysaccharide was obtained by using a carbodiimide‐like activating agent for carboxylic groups and 2‐aminoethyl‐phosphonic acid to create an amide bond between the amine of the phosphonate agent and the carboxylic acids of CMC. The polymer was characterized by 31P NMR, FT‐IR, and potentiometric titration. CMCAPh showed different properties from CMC and its amidated derivative polymer CMCA. The behavior in solution of CMCAPh polymer towards normal human osteoblasts (NHOst) was studied in vitro, monitoring the cell proliferation, cell differentiation, and osteogenic activity and was then compared with the amidic derivative of carboxymethylcellulose (CMCA). Furthermore, CMCAPh was used to coat titania disks with the aim of increasing the osteogenic activity of implant surfaces. The polymer film on the titania surface was characterized by AFM and TOF‐SIMS analysis. An ATR FT‐IR study was carried out to evaluate the polymer bonding mode onto the titanium surface. Osteoblast morphology was evaluated by SEM. Adhesion analysis of NHOst demonstrated a better adhesion on the titanium surface coated with CMCAPh than on the bare titanium surface. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010
Corrosion behavior of nitinol in blood serum and PBS containing amino acids
Authors: Pound Bruce G. 
Published: June 2010

AbstractThe susceptibility to localized corrosion of metallic, implant medical devices is typically evaluated in a simulated physiological solution, such as phosphate buffered saline (PBS). For implant devices in contact with blood, the underlying premise is that proteins and other components in blood do not significantly change the corrosion susceptibility. This study examined the corrosion behavior of nitinol in bovine serum and PBS containing amino acids (cysteine, glutamine, and tryptophan). Tests were performed on mechanically polished (MP) and black oxide (BO) nitinol wire using cyclic potentiodynamic polarization and electrochemical impedance spectroscopy. They showed that the susceptibility to pitting corrosion of MP nitinol in PBS can be influenced by the presence of amino acids, depending on the type of amino acid. However, the pitting susceptibility of MP and BO nitinol did not differ significantly (based on a t‐test, with p < 0.05 being statistically significant) between serum and PBS, suggesting that the combination of proteins and amino acids in serum had little effect. The impedance spectra showed near‐capacitive behavior in serum and in PBS alone and with amino acids, and the data could be fitted by a parallel resistance–capacitance (as a constant phase element) circuit associated with the passive oxide film. The capacitance indicated that serum proteins were adsorbed on nitinol but that little, if any, adsorption of the three amino acids under study occurred at the corrosion potential. There did not appear to be a correlation between breakdown and the adsorption of organic compounds in serum. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010.
Plasma filtration in the treatment of graves' ophthalmopathy: A randomized study
Authors: Cap J. , Ceeova V. , Skacha M. , Rezek P. , Vlcek P. , Blaha M. 
Published: June 2010

AbstractThe aim of this study was to perform a randomized study to evaluate the role of plasma filtration in the treatment of severe thyroid‐associated ophthalmopathy (TAO). 20 patients were enrolled, and all patients were treated with methylprednisolone IV pulses. 10 randomly chosen patients were also subjected to plasma filtration (twice weekly in Weeks 1, 2, 4, 7, and 10). The procedure proved to be safe. All immunoglobulin classes as well as autoantibodies directed against thyroglobulin, thyroid peroxidase, and TSH receptor exhibited statistically significantly decreases. Some markers of cell‐mediated immunity such as soluble antigen CD30 and monocyte chemotactic protein 1 decreased, but serum levels of other markers such as CD40 ligand and soluble protein Fas/Apo‐1 did not change significantly. The decrease of immunoglobulins was long lasting, whereas cytokine levels returned to basal values before the next apheresis. Although the clinical activity score (CAS) dropped in all patients, it occurred more rapidly in patients treated with plasma filtration. The CAS difference between the two groups was statistically significant (p = 0.027). The amplitude of visual evoked potentials improved after 3 months in the plasma filtration group. At the end of the study, there was no difference between patients treated with aphereses and the control group. Eye muscle width and proptosis measured by CT scan did not differ between the two groups. We conclude that apheresis can decrease disease activity more rapidly than standard high‐dose IV glucocorticoid therapy. Whether this superior treatment effect could potentially avoid surgical procedures remains to be determined. J. Clin. Apheresis 25:209–215, 2010. © 2010 Wiley‐Liss, Inc.
Epithelial stem cells: An epigenetic and wnt‐centric perspective
Authors: Gu Bingnan , Watanabe Kazuhide , Dai Xing 
Published: June 2010

AbstractEpithelial stem cells, such as those present in mammalian skin, intestine, or mammary gland, are tissue stem cells capable of both long‐term self‐renewal and multi‐lineage differentiation. Here we review studies implicating epigenetic control mechanisms in mammalian epithelial stem cell development and homeostasis. We also provide an update of recent progresses in the involvement of canonical Wnt signaling and note an interesting link between the Wnt pathway and chromatin regulation in epithelial stem cells. We anticipate that epigenetic and epigenomic studies of these cells will increase exponentially in the near future. J. Cell. Biochem. 106: 1279–1287, 2010. © 2010 Wiley‐Liss, Inc.
Twist‐ing cell fate: Mechanistic insights into the role of twist in lineage specification/differentiation and tumorigenesis
Authors: Cakouros D. , Raices R. M. , Gronthos S. , Glackin C.A. 
Published: June 2010

AbstractBone marrow‐derived mesenchymal stem cells (MSC), are multipotent cells that give rise to multiple lineages including osteoblasts, adipocytes, muscle, and fibroblasts. MSCs are useful for clinical applications such as cell therapy because they can be isolated from an individual and expanded for use in tissue repair, as well as other therapeutic applications, without immune rejection. However, one of the key problems in the use of MSCs for these applications is the efficiency of these cells to engraft and fully regenerate damaged tissues. Therefore, to optimize this process, a comprehensive understanding of the key regulators of MSCs self‐renewal and maintenance are critical to the success of future cell therapy as well as other clinical applications. The basic helix loop helix transcription factor, Twist, plays a master regulatory role in all of these processes and, therefore, a thorough understanding of the mechanistic insights in the role of Twist in lineage specification/differentiation and tumorigenesis is vital to the success of future clinical applications for the therapeutic use of MSCs. In this article, we highlight the basic mechanisms and signaling pathways that are important to MSC fate, maintenance, and differentiation, as well as the critical role that Twist plays in these processes. In addition, we review the known literature suggesting a critical role for Twist in the generation of cancer stem cells, as this information may contribute to a broader understanding of stem cell biology and stem‐cell‐based therapeutics. J. Cell. Biochem. 110: 1288–1298, 2010. © 2010 Wiley‐Liss, Inc.
Proliferative arrest of neural cells induces prion protein synthesis, nanotube formation, and cell‐to‐cell contacts
Authors: Miyazawa Kohtaro , Emmerling Kaitlin , Manuelidis Laura 
Published: June 2010

AbstractHost prion protein (PrP) is most abundant in neurons where its functions are unclear. PrP mRNA transcripts accumulate at key developmental times linked to cell division arrest and terminal differentiation. We sought to find if proliferative arrest was sufficient to cause an increase in PrP in developing neurons. Rat neuronal precursor cells transduced with the temperature sensitive SV‐40 T antigen just before terminal differentiation (permissive at 33°C but not at 37.5°C) were analyzed. By 2 days, T antigen was decreased in all cells at 37.5°C, with few DNA synthesizing (BrdU+) cells. Proliferative arrest induced by 37.5°C yielded a fourfold PrP increase. When combined with reduced serum, a sevenfold increase was found. Within 2 days additional neuritic processes with abundant plasma membrane PrP connected many cells. PrP also concentrated between apposed stationary cells, and on extending growth cones and their filopodia. Stationary cells were maintained for 30 days in their original plate, and they reverted to a proliferating low PrP state at 33°C. Ultrastructural studies confirmed increased nanotubes and adherent junctions between high PrP cells. Additionally, some cells shared cytoplasm and these apparently open regions are likely conduits for the exchange of organelles and viruses that have been observed in living cells. Thus PrP is associated with dynamic recognition and contact functions, and may be involved in the transient formation of neural syncytia at key times in embryogenesis. This system can be used to identify drugs that inhibit the transport and spread of infectious CJD particles through the nervous system. J. Cell. Biochem. 111: 239–247, 2010. © 2010 Wiley‐Liss, Inc.
Using social network analysis to evaluate community capacity building of a regional Community Cancer Network
Authors: Luque John , Martinez Tyson Dinorah , Lee Ji‐Hyun , Gwede Clement , Vadaparampil Susan , Noel‐Thomas Shalewa , Meade Cathy 
Published: June 2010

AbstractThe Tampa Bay Community Cancer Network (TBCCN) is one of 25 Community Network Programs funded by the National Cancer Institute's (NCI's) Center to Reduce Cancer Health Disparities with the objectives to create a collaborative infrastructure of academic and community based organizations and to develop effective and sustainable interventions to reduce cancer health disparities. In order to describe the network characteristics of the TBCCN as part of our ongoing evaluation efforts, we conducted social network analysis surveys with our community partners in 2007 and 2008. One key finding showed that the mean trust value for the 20 community partners in the study increased from 1.8 to 2.1 (p<0.01), suggesting a trend toward increased trust in the network. These preliminary results suggest that TBCCN has led to greater collaboration among the community partners that were formed through its capacity‐building and evidence‐based dissemination activities for impacting cancer health disparities at the community level. © 2010 Wiley Periodicals, Inc.
Advanced oxidation protein products inhibit differentiation and activate inflammation in 3T3‐L1 preadipocytes
Authors: Zhou Qiu Gen , Peng Xin , Hu Li Li , Xie Di , Zhou Min , Hou Fan Fan 
Published: June 2010

AbstractAccumulation of advanced oxidation protein products (AOPPs) is prevalent in metabolic syndromes, a condition with impaired preadipocytes differentiation. In the present study, we tested the hypothesis that AOPPs disturb preadipocyte differentiation. Exposure of 3T3‐L1 preadipocytes to increased levels of AOPPs inhibited accumulation of intracellular triglyceride and decreased the expression of the essential markers of matured adipocytes, such as adipocyte fatty‐acid‐binding protein (aP2), CAAT/enhancer‐binding protein (C/EBP)‐α, and peroxisome proliferator‐activated receptor (PPAR)‐γ, in response to standard adipogenic induction. Inhibitory effects of AOPPs on preadipocytes differentiation was time sensitive, which occurred at the early stage of differentiation. In the presence of AOPPs, induction of preadipocytes differentiation resulted in upregulated expression of C/EBP homologous protein (CHOP) and CUG‐Triplet repeat‐binding protein (CUGBP), two important inhibitors of preadipocytes differentiation. In addition, treatment with AOPPs increased abundance of C/EBP‐β‐liver enriched inhibitory protein (C/EBP‐β‐LIP), a truncated C/EBP‐β isoform without adipogenic activity. Moreover, AOPPs‐treated preadipocytes expressed a macrophage marker F4/80 and overexpressed tumor necrosis factor‐α and interleukin‐6 via nuclear factor‐κB (NF‐κB)‐dependent pathway. However, blocking inflammation with NF‐κB inhibitor failed to improve AOPPs‐induced inhibition of preadipocytes differentiation. These data suggest that accumulation of AOPPs may inhibit differentiation of preadipocytes and activate inflammation in these cells. This information might have implication for understanding the impairment of preadipocytes differentiation and fat inflammation seen in metabolic syndrome. J. Cell. Physiol. 225: 42–51, 2010. © 2010 Wiley‐Liss, Inc.
Control of carbohydrate metabolism in an anoxia‐tolerant nervous system
Authors: de Fraga Luciano Stürmer , da Silva Roselis Silveira Martins , Zancan Denise Maria 
Published: June 2010

AbstractAnoxia‐tolerant animal models are crucial to understand protective mechanisms during low oxygen excursions. As glycogen is the main fermentable fuel supporting energy production during oxygen tension reduction, understanding glycogen metabolism can provide important insights about processes involved in anoxia survival. In this report we studied carbohydrate metabolism regulation in the central nervous system (CNS) of an anoxia‐tolerant land snail during experimental anoxia exposure and subsequent reoxygenation. Glucose uptake, glycogen synthesis from glucose, and the key enzymes of glycogen metabolism, glycogen synthase (GS) and glycogen phosphorylase (GP), were analyzed. When exposed to anoxia, the nervous ganglia of the snail achieved a sustained glucose uptake and glycogen synthesis levels, which seems important to maintain neural homeostasis. However, the activities of GS and GP were reduced, indicating a possible metabolic depression in the CNS. During the aerobic recovery period, the enzyme activities returned to basal values. The possible strategies used byMegalobulimus abbreviatusCNS to survive anoxia are discussed.J. Exp. Zool. 313A:539–547, 2010. © 2010 Wiley‐Liss, Inc.
Carrier cell‐mediated cell lysis of squamous cell carcinoma cells by squamous cell carcinoma antigen 1 promoter‐driven oncolytic adenovirus
Authors: Hamada Katsuyuki , Zhang Ting , Desaki Junzo , Nakashiro Koh‐ichi , Itoh Hiroshi , Tani Kenzaburo , Koyama Yoshiyuki , Hamakawa Hiroyuki 
Published: June 2010

AbstractBackgroundThe squamous cell carcinoma antigen (SCCA) serves as a serological marker for squamous cell carcinomas. Molecular cloning of the SCCA genomic region has revealed the presence of two tandemly arrayed genes: SCCA1 and SCCA2. SCCA1 gene is up‐regulated in squamous cell carcinoma cells. We analyzed the proximal region of the SCCA1 promoter and the antitumor effect of oncolytic adenovirus driven by the SCCA1 promoter in squamous cell carcinoma cells.MethodsThe SCCA1 promoter was analyzed by dual luciferase assay and substituted with the E1A promoter to construct the oncolytic adenovirus to determine the squamous cell carcinoma‐specific cell lysis.ResultsDeletion analysis of SCCA1 promoter identified a 175‐bp core promoter region and an enhancer region at −525 to −475 bp upstream of the transcription start site. The transcriptional activity of the SCCA1 promoter was up‐regulated in squamous cell carcinoma cells. Five tandem repeats of enhancer increased SCCA1 promoter activity by four‐fold. Oncolytic adenovirus driven by this SCCA1 enhancer‐promoter complex specifically killed squamous cell carcinoma cells in vitro and in vivo. A549 carrier cells infected with the oncolytic adenovirus induced complete regression of syngeneic squamous cell carcinoma cell tumor by overcoming immunogenicity and adenovirus‐mGM‐CSF augmented the antitumor effect of carrier cells.ConclusionsSCCA1 was up‐regulated in squamous cell carcinoma cells and oncolytic adenovirus driven by SCCA1 promoter specifically killed these cells. These findings suggest that SCCA1 promoter is a potential target of gene therapy for squamous cell carcinoma. Copyright © 2010 John Wiley & Sons, Ltd.
Change of the favored routes of EI MS fragmentation when proceeding from N1, N1‐dimethyl‐N2‐arylformamidines to 1,1,3,3‐tetraalkyl‐2‐arylguanidines: substituent effects
Authors: Raczyńska Ewa D. , Makowski Mariusz , Gal Jean‐François , Maria Pierre‐Charles 
Published: June 2010

AbstractAlthough series of N1, N1‐dimethyl‐N2‐arylformamidines and of 1,1,3,3‐tetraalkyl‐2‐arylguanidines are structurally analogous and similar electron‐ionization mass spectral fragmentation may be expected, they display important differences in the favored routes of fragmentation and consequently in substituent effects on ion abundances. In the case of formamidines, the cyclization‐elimination process (initiated by nucleophilic attack of the N‐amino atom on the 2‐position of the phenyl ring) and formation of the cyclic benzimidazolium [M‐H]+ ions dominates, whereas the loss of the NR2 group is more favored for guanidines. In order to gain information on the most probable structures of the principal fragments, quantum‐chemical calculations were performed on a selected set. A good linear relation between log{I[M‐H]+I [M]+•} and σR+ constants of substituent at para position in the phenyl ring occurs solely for formamidines (r = 0.989). In the case of guanidines, this relation is not significant (r = 0.659). A good linear relation is found between log{I[M‐NMe2]+/I [M]+•} and σp+ constants (r = 0.993). Copyright © 2010 John Wiley & Sons, Ltd.