bluuebunny/crossref_metadata_2025_split huggingface dataset:
PSIV-A-10 Pilot study: Acute changes in mare milk composition in response to concentrate feeding
Authors: Oberlin Carlee , Zimmerman-Cameron Savea , Pyles Morgan , Gerbracht Izabell H 
Published: May 2024

Abstract
Evaluating dietary effects on mare milk composition is important for optimizing nutrition programs for broodmares. Additionally, accurately measuring milk composition is important for many research studies. Different techniques have been used to collect milk samples, however, the effect time of collection after feeding has not been investigated. The objective of this study was to evaluate the short-term changes in mare milk composition after a concentrate meal. We hypothesized that milk composition, specifically lactose, follows a similar pattern as blood glucose after a high starch meal. Milk samples were collected from four mares at 6 to 7 d postpartum. Baseline blood samples and milk samples were collected, then mares were fed a concentrate meal containing 0.697 g starch/kg body weight (BW). Blood samples were collected via jugular venipuncture at 60, 120, 240, 360, and 480 min postprandial. Blood glucose was measured using a glucometer (OneTouch Ultra 2, LifeScan, USA). Milk samples were collected every 60 min for 480 min postprandial. Foals were muzzled for 60 min intervals and allowed to nurse at each time point to induce milk ejection. Milk samples (&gt; 50 mL) were collected by hand while foals nursed. Milk lactose was measured using an infrared-based milk analyzer (MilkoScan FT+, Foss, Denmark). Area under the curve for lactose and glucose were calculated using the trapezoid method. Changes over time in milk lactose and blood glucose were evaluated using mixed model ANOVA with repeated measures. Pearson’s correlation coefficient was used to evaluate relationships between variables (SAS 9.4). Results were considered significant when P &lt; 0.05. There was a change over time in milk lactose after the concentrate meal (P &lt; 0.0001). Milk lactose was the least pre-feeding (59.0 ± 0.50 g/L) and greatest between 240 to 360 min postprandial (61.6 ± 0.47 g/L; P &lt; 0.05). Blood glucose changed over time after the concentrate meal (P = 0.0365). Pre-feeding glucose was 88.25 ± 2.10 mg/dL which increased to 126.75 ± 10.82 mg/dL by 60 min postprandial (P &lt; 0.05). By 240 min blood glucose was similar to pre-feeding (91.25 ± 6.90 mg/dL; P &gt;0.05). Mean blood glucose AUC was 663 and mean milk lactose AUC was 834. Time to peak glucose was 82.5 min whereas time to peak milk lactose was 225 min. There was no relationship between peak blood glucose and peak milk lactose (P = 0.7311) or AUC for blood glucose or milk lactose (P = 0.8256). The lack of significant relationships may be due to the small sample size. The results from this pilot study demonstrated short term changes in milk lactose after a concentrate meal. Collecting milk samples between 240 to 360 min may be suitable to evaluate dietary effects on milk lactose. However, additional research is needed to evaluate other milk components.
PSIV-A-1 Comparing early and late spring grazing of cereal rye utilizing a high-density, short-duration grazing system
Authors: Stone Rachel , McCann Joshua C , Trennepohl Keela 
Published: May 2024

Abstract
The objective of this study was to compare forage yield, nutritive value, grazing forage disappearance, in situ dry matter (DM) disappearance, and neutral detergent fiber (NDF) digestibility between late- and early-grazed cereal rye. Cover crop grazing time varies from year to year due to environmental and management conditions. Cereal rye is a high-yielding forage cover crop; however, rye matures quickly and subsequently declines in nutritive value. This experiment was conducted at Western Illinois University Kerr Farm, Macomb, IL. The late-grazed rye was planted October 9, 2020, while the early-grazed rye was planted November 8, 2022. Rye was planted at a rate of 90 kg/ha with a no-till drill following corn harvest. In yr 1, cereal rye was grazed and sampled during anthesis (mid to late May). In yr 2, cereal rye was grazed and sampled during the late boot stage (in mid to late April). A high-density, short-duration grazing system was used in both years. Cow-calf pairs (n = 58 pairs) grazed 0.40-ha plots for 24 h per plot. Plots were randomly sampled in four locations before and after grazing within a 0.40 m2 forage ring to determine forage nutritive value, height, and yield. All grass within the ring was clipped at approximately 12 cm to mimic grazing. Samples were analyzed at Rock River Laboratories (Watertown, WI) for crude protein (CP), neutral detergent fiber (NDF), acid detergent fiber (ADF), and neutral detergent fiber digestibility (NDFD) at 12, 30, and 48 h. In situ DM disappearance was determined using ruminally cannulated beef heifers. Data were analyzed using the MIXED procedure of SAS 9.4. Early-grazed rye had a greater (P &lt; 0.01) CP and less (P &lt; 0.01) NDF and ADF than late-grazed rye. Accordingly, early-grazed rye had more than 50% greater (P &lt; 0.01) DM disappearance at 24 and 48 h compared with late-grazed rye. Moreover, early-grazed rye had greater (P &lt; 0.01) NDFD at 12, 30, and 48 h. However, late-grazed rye was 108 cm taller (P &lt; 0.01) before grazing and remained 10 cm taller (P = 0.04) than early-grazed rye after grazing. Late-grazed rye yielded (P &lt; 0.01) more DM than early-grazed rye, 3,873 vs. 1,079 kg/ha, respectively. After grazing, more DM (P &lt; 0.01) remained on early-grazed rye plots than late-grazed rye plots, 496 vs. 2,445 kg/ha, respectively. Cows consumed 20% more DM (P = 0.04) of the early-grazed rye compared with late-grazed rye. Overall, an early-grazed rye cover crop was more digestible with a greater nutritive value and resulted in greater grazing consumption using a high-density, short-duration grazing system.
PSIV-A-4 Effect of late gestational nutrient restriction on locomotor activity of beef heifers
Authors: DeHaven Mattie T , Redifer Colby A , Rathert-Williams Abigail R , Meyer Allison M 
Published: May 2024

Abstract
The objective of this study was to determine the effects of nutrient restriction on locomotor behavior during late gestation. Fall-calving crossbred beef heifers were moved to 3.7 × 15.8 m partially-covered pens and individually-fed 100% (control; n = 13) or 70% (nutrient restricted; n = 13) of NASEM metabolizable energy and metabolizable protein requirements for maintenance, pregnancy, and growth from d 160 of gestation to calving. Diets were based on chopped sorghum sudan hay fed in Calan gates and supplemented to meet targeted nutritional planes. IceQube accelerometers (iceRobotics, Edinburgh, UK) were placed on the left hind fetlock between d 158 and 168 of gestation to determine standing time, lying time, step count, number of lying bouts, and motion index. Daily behavior data were averaged for 5 distinct periods (period 1: IceQube placement to d 180 of gestation; period 2: d 181 to 201 of gestation; period 3: d 202 to 222 of gestation; period 4: d 223 to 243 of gestation; and period 5: 244 to 264 of gestation). Days in which animals were moved from their pens were excluded from the data set, resulting in an average of 17.2 ± 2.35 d per period. Data were analyzed with fixed effects of nutritional plane, period, and their interaction in the model. Maternal sire and day of treatment initiation were included as covariates. The number of lying bouts was affected (P = 0.02) by the nutritional plane × period interaction. Within period, there was no effect (P ≥ 0.20) of nutritional plane on lying bouts. Lying bouts in control heifers decreased (P = 0.008) from period 1 to 2, whereas lying bouts in nutrient restricted heifers decreased (P = 0.002) from period 1 to 2 before increasing (P = 0.02) again from period 2 to 3. Motion index and step count were affected (P ≤ 0.05) by nutritional plane, where both were greater in control heifers. Motion index and step count were affected (P &lt; 0.001) by period, while standing time and lying time tended to be affected (P ≤ 0.09) by period. Motion index and step count decreased (P &lt; 0.001) between periods 1 and 2 and periods 3 and 4 but increased (P ≤ 0.004) between periods 2 and 3 and periods 4 and 5. Standing time decreased (P = 0.02), while lying time increased (P = 0.02), between periods 3 and 4. These data suggest that nutrient restricted heifers may have reduced locomotor behavior in late gestation compared with control heifers. Throughout all periods of gestation, control heifers took more daily steps and had a greater motion index, suggesting that nutrient availability in late gestation may directly impact locomotor behavior of beef heifers.
PSIV-A-6 Effects of a yeast cell wall additive on the growth performance and fecal Eimeria load of finishing lambs
Authors: Kahler Kristen , Dahmer Payton L 
Published: May 2024

Abstract
Yeast-based additives have been shown to alter the rumen environment and exhibit immunomodulatory properties that can lead to enhancements in health status and subsequent performance. The objectives of this study were to evaluate the impacts of supplementing a yeast cell wall additive (Safwallâ, Phileo by Lesaffre, Milwaukee, WI) on finishing lamb growth performance and fecal Eimeria load. A total of 44 Polypay lambs were used in a 35-dexperiment at the Kansas State University Sheep and Meat Goat Center. On d 0, a fresh fecal sample was collected from each lamb and submitted to the Kansas State University Veterinary Diagnostic Lab to determine a baseline Eimeria count via quantitative fecal float. Lambs were then randomly assigned to either a control diet or diet supplemented with 1.5 g Safwallâ. Diets were fed for 35-d and contained no coccidiostat. Lambs had ad libitum access to both feed and water for the duration of the experiment. A basal lamb finisher diet was manufactured as a control diet and Safwallâ was hand-mixed into rations daily to make the treatment diet. Lambs and feeders were individually weighed weekly to track dry matter intake (DMI) and changes in body weight (BW). Data were analyzed using the GLIMMIX procedure of SAS (v.9.4, SAS Inst., Cary, NC) with an alpha set at (P &lt; 0.05). From d 0 to 7 there was no evidence of a difference (P = 0.19) in DMI between treatment groups; however, during the second week of the study (d 7 to 14) lambs fed the control diet had significantly greater (P = 0.02) DMI compared with those fed a diet supplemented with yeast cell wall (Table 1). There was no evidence of differences in DMI between treatments for the remainder of the study (P &gt; 0.05). Likewise, BW did not differ significantly (P &gt; 0.05) between treatments on d 0 or by d 35. There was no evidence that fecal Eimeria load differed significantly (P &gt; 0.05) on d 0 or 21; however, on d 35, lambs fed the control diet had a decreased Eimeria load compared with those fed a diet with yeast cell wall, but neither group had biologically relevant levels of Eimeria present in the feces. In summary, feeding a diet containing a yeast cell wall additive did not alter finishing lamb growth performance. While lambs fed the control diet had reduced fecal Eimeria at the end of the study, there was a reduction in this variable across both treatments. Further research should be conducted with younger animals with a known Eimeria challenge.
PSIV-A-3 Effect of fiber and stimbiotic on growth performance and the gut microbiome in pigs challenged with F18 enterotoxigenic Escherichia coli (ETEC)
Authors: Shirer Erika , Watson Alisun N , Story Robert , Maxwell Charles V V , Becker Spenser , Wilcock Pete , Gerhart Amanda J , Zhao Jiangchao , Tsai Tsungcheng Cheng 
Published: May 2024

Abstract
Dietary fiber has been shown to promote a healthy gut microbiota, while stimbiotics enhance fermentable substances and boost the expansion of the commensal microbiome, improving host health by alleviating deleterious effects of proinflammatory responses. The intended purpose of this study was to gain an understanding of the effectiveness of dietary fiber sources and stimbiotic on the sustainability of F18 enterotoxigenic Escherichia coli (ETEC) challenge. A total of 216 piglets were blocked (n = 8) by weaning body weight (BW) and randomly assigned to 1 of 4 dietary treatments, including corn-SBM based control diet (CC), high fiber diet (HF: 5.0, 3.8. 2.5% sugar beet pulp and 4.0, 3.0 and 2.0% soy hulls for phases 1, 2, and 3, respectively), CC plus 0.01% stimbiotic (CS: Signis, ABVISTA Marlborough, UK), and HF plus 0.01% stimbiotic (FS). Pigs were fed a four-phase feeding regimen: phase 1 (d 0-9), phase 2 (d 9-16), phase 3 (d 16-22), phase 4 (d 22-41). Dietary fiber and stimbiotic were provided from weaning (d 0) to d 22, and then a common diet was fed from d 22 to the end of the trial. On d 5 postweaning, all pigs were orally inoculated with 10 mL of F18 ETEC (108 CFU/mL). One median BW pig from each pen was selected at weaning for fecal sampling. Before the challenge, the same pigs were sampled on d 5 and again on d 8 and d12. Samples were used to quantify the E. Coli using 3M Petrifilm (3M Food Safety, St. Paul, MN) and virulence genes of the isolate were qualified using multiplex PCR. Colony-forming-count was log-transformed. All data normality was verified using Univariate procedure and analyzed using Mixed procedure of SAS (Cary, NC). Pigs fed both CS and HF increased average daily gain (ADG) in phase 2 and phase 1-3 compared with pigs fed CC diets, while adding stimbiotic had no additional benefit to HF (Fiber×Stimbotic P = 0.03 and 0.06, respectively). This response led to heavier BW in pigs fed CS at the end of phase 3 compared with pigs fed CC with HF and FS as intermediate (Fiber×Stimbotic P = 0.06, respectively). Overall, average daily feed intake (ADFI) was greater in pigs fed CS than pigs fed FS while no difference was found between CC and CF (Fiber×Stimbotic P = 0.02). Dietary fiber did not impact gain to feed ratio (G:F); however, G:F was improved in stimbiotic fed pigs in phase 2 (Stimbiotic P = 0.01). Total coliform count was reduced from d 2 to d 7 (day; P &lt; 0.01), but neither fiber nor stimbiotic altered the coliform count. Results of this study demonstrated that both high fiber and stimbiotic help restore ADG when pigs were challenged with ETEC. Additionally, stimbiotic also benefited feed efficiency and resulted in the heaviest BW.
PSIV-A-11 Potential of black soldier fly (Hermetia illucens) larvae frass as a functional ingredient for nursery pigs
Authors: Rubio Sarah , Kuneff Izadora Batista , Soler Fernando , Espinosa Charmaine , Koutsos Liz , van Heugten Eric 
Published: May 2024

Abstract
Frass is the leftover material from the production of black soldier fly larvae (BSFL), which includes chitin-containing exoskeleton shedding, small, processed larvae, leftover dietary substrate, and larvae excrement. A total of 120 nursery pigs (initial body weight of 6.85 ± 0.10 kg) weaned at 21 d of age were used in a 6-wk study to evaluate the effect of increasing dietary levels of frass on growth performance, diarrhea score, and serological indices. Pigs were randomly assigned within sex and body weight blocks to 1 of 3 dietary treatments using 30 pens (4 pigs/pen; 10 replicates/treatment). Diets were prepared from a common basal and fed in 3 dietary phases of 2 wk each and included: 1) a control diet based primarily on corn and soybean meal; 2) the control with 3% added frass; and 3) the control with 6% added frass. On d 4, 7, and 14, individual fecal samples were obtained using fecal loops and were evaluated on a scale of 1 to 5 representing hard, normal, soft, watery soft, and watery liquid feces, respectively. Blood samples were collected on d 42 from 1 median pig per pen for the analysis of serum chemistry. Data were analyzed using the mixed model procedures as a randomized complete block design using linear and quadratic contrast comparisons to determine the effects of frass. Supplementation of frass linearly increased average daily gain (ADG) from d 7 to 14 (P = 0.044; 347.9, 378.8, and 396.8 g/d for 0, 3, and 6% frass, respectively), and tended to linearly increase ADG during Phase 1 (P = 0.079; 265.1, 284.8, and 298.6 g/d). No differences were observed for final body weight (P ≥ 0.146; 29.90, 31.48, and 30.70 kg for 0, 3, and 6% frass, respectively), and overall ADG, average daily feed intake (ADFI), or gain to feed ratio (G:F). Diarrhea scores increased (P &lt; 0.001) from d 4 (2.96) to d 7 (4.20) and then decreased (P &lt; 0.001) on d 14 (3.69). Inclusion of frass at 6% had a decreased diarrhea score compared with 3% frass on d 7 (P = 0.029), but pigs fed 6% frass had a greater diarrhea score (P = 0.038) than pigs fed the control diet on d 14. However, scores were indicative of diarrhea for all treatments on d 7 and 14. Serum concentrations of total protein, albumin, globulin, aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, γ-glutamyltranspeptidase, urea N, creatinine, glucose, Ca, P, Mg, K, Na, Cl, triglycerides, amylase, cholesterol, and creatine phosphokinase measured on d 42 were not different (P &gt; 0.093) between treatments. All serological analytical results were generally within normal expected ranges. In conclusion, these findings suggest that dietary inclusion of frass may improve growth performance of nursery pigs, especially during the early stages of the nursery period.
PSIV-A-13 The relationship between breed-wide expected progeny differences, genomic predictors, and phenotypic values in mature Angus cows
Authors: Riddle Jacy , Holder Amanda 
Published: May 2024

Abstract
For many years, selection for improved beef production centered on heavier weaning weights and more rapid post-weaning gain. More recently, measurement of post-weaning feed intake has facilitated direct improvement in feed efficiency in the feedlot. However, intake on a high concentrate diet is controlled by different biological mechanisms than intake on a high roughage diet. For this reason, the question remains whether an animal that efficiently converts calories from a high-quality concentrate-based ration similar to that of a feedlot diet where the expected progeny differences (EPDs) and genomic predictors (GPs) are developed would also be an efficient forage utilizer as a mature cow. To evaluate this relationship, we compared breed-wide EPDs and predictors to performance on a roughage diet and on a concentrate diet in mature cows. The objective of this study was to determine if EPDs and GPs developed and used in feedlots are applicable to commercial cow/calf producers utilizing forages and other roughages as the main diet ingredient. Data for this study were collected at Oklahoma State University from the fall of 2018 to the spring of 2021. Tissue samples from 64 mature Angus cows were submitted to Neogen for profiling according to genetic potential for 16 different traits. In each trial, retained energy (RE), dry matter intake (DMI), milk production (MILK), mature weight (MW), and average daily gain (ADG) were recorded and compared with the corresponding EPD and GP. Pearson correlations were determined between phenotypic values, GPs, and EPDs for both diets on each trial. No EPD or GP was significantly correlated to any measured trait on the forage diet. The DMI EPD was strongly correlated with DMI on a concentrate-based diet, however, did not predict DMI on a forage diet in mature cows. Based on the evaluations performed in this study, selecting for animals that perform well in the feedlot may be inadvertently selecting for mature cows that perform poorly on a typical mature cow diet of medium to low-quality forages.
PSIV-A-12 Ruminal in vitro digestibility of novel pennycress meal using the Daisy II incubator
Authors: Brown Alyssa , Prybylski Emma G , Neuleib Lauren , McCann Joshua C , Heller Nicholas , Earing Jennifer E 
Published: May 2024

Abstract
Field pennycress seed has a high oil content, making it valuable for biofuel production. The high protein content of the resulting pennycress meal makes it a potentially valuable protein feedstuff for livestock. However, pennycress meal also contains greater content of fiber, erucic acid, and glucosinolates, which limit its use in livestock rations. Golden pennycress, a novel pennycress, was developed to contain greater oil content and decreased content of fiber, increasing the value of pennycress meal as a potential livestock feed. The objective of this research was to characterize a novel pennycress meal using an in vitro rumen fermentation system. Digestion of golden pennycress (GPC), black-seeded pennycress (BPC), and a total mixed ration (TMR, as the control), was evaluated in vitro using the Daisy II incubator. Each incubation vessel contained 12 filter bags (4 containing GPC, 4 containing BPC, and 4 containing the TMR). Rumen fluid was collected from two cannulated steers and processed according to Ankom procedures; samples were incubated in the rumen fluid inoculum for either 24 or 48 h. Neutral Detergent Fiber (NDF) of GPC averaged 23% (dry matter basis) and was less than the BCP NDF content (30%, dry matter basis). In vitro true dry matter disappearance (IVTDMD) and in vitro neutral detergent fiber digestibility (NDFD) were calculated and shown in Table 1. Proc Mixed procedures of SAS were used to identify difference in digestibility. Due to the novelty of golden pennycress, this study appears to be the first that compares ruminal digestibility of pennycress meals, thereby establishing initial data relative to the feeding value of GPC. As the novel pennycress was developed to have a reduced fiber level, the reduced NDF content of GPC over BPC was expected. The significant increase of IVTDMD of GPC compared with BPC at either incubation length suggests increased overall digestibility and improved dry matter utilization of the novel pennycress. Additional research investigating the reduced NDFD of GPC is warranted. As the first study evaluating the use of golden pennycress meal in ruminant diets, this study provides baseline values from which to build additional research projects.
Antibody-drug conjugates in solid tumors; new strategy for cancer therapy
Authors: Takakura Toshiaki , Shimizu Toshio , Yamamoto Nobuyuki 
Published: May 2024

Abstract
Antibody-drug conjugates (ADCs) have emerged as a novel class of anticancer treatment. ADCs are composed of three parts: a monoclonal antibody, a linker and a payload. A monoclonal antibody binds to the specific antigen present at the cancer cells, allowing selective delivery of the cytotoxic agents to the tumor site. Several ADCs are approved by the US Food and Drug Administration for the treatment of hematologic cancers and solid tumors with clinically meaningful survival benefit. However, the development of ADCs faces a lot of challenges and there is a need to get better understanding of ADCs in order to improve patient outcomes. Here, we briefly discuss the structure and mechanism of ADCs, as well as the clinical data of current approved ADCs in solid tumors.