bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of Geothermal Fumaroles in Driving the Microbial Community Dynamics and Functions of Adjacent Ecosystems
Authors: Asif Aslia , Chen Jung‐Sheng , Hsu Gwo‐Jong , Hussain Bashir , Nagarajan Viji , Koner Suprokash , Huang Shih‐Wei , Hsu Bing‐Mu 
Published: June 2024

ABSTRACTGrowing evidence suggests that the hydrochemical properties of geothermal fumaroles may play a crucial role in shaping the diversity and functions of microbial communities in various environments. In the present study, the impact of geothermal furaneols on the microbial communities and their metabolic functions across the rock‐soil‐plant continuum was explored considering varying distances from the fumarole source. The results revealed that bacterial phylum Proteobacteria was predominant in all sample types, except in the 10 m rock sample, irrespective of the sampling distance. Archaeal phyla, such as Euryarchaeota and Crenarchaeota, were more prevalent in rock and soil samples, whereas bacterial phyla were more prevalent in plant samples. Thermoacidophilic archaeons, including Picrophilus, Ferroplasma, and Thermogymnomonas were dominant in rocks and soil samples of 1 and 5 m distances; acidophilic mesophiles, including Ferrimicrobium and Granulicella were abundant in the rhizoplane samples, whereas rhizosphere‐associated microbes including Pseudomonas, Pedobacter, Rhizobium, and Novosphingobium were found dominant in the rhizosphere samples. The functional analysis highlighted the higher expression of sulfur oxidative pathways in the rock and soil samples; dark iron oxidation and nitrate/nitrogen respiratory functions in the rhizosphere samples. The findings underscore microbial adaptations across the rock‐soil‐plant continuum, emphasizing the intricate relationship between geothermal fumaroles and microbial communities in adjacent ecosystems. These insights offer a crucial understanding of the evolution of microbial life and highlight their pivotal roles in shaping ecosystem dynamics and functions.
The psychological impact of the COVID‐19 pandemic on Black social workers
Authors: Collins Daniel G. , Holman Alea R. , Hsu Tina S. 
Published: June 2024

AbstractThis qualitative study investigated the psychological impact of the dual pandemics of COVID‐19 and anti‐Black violence on client‐facing social workers who identify as Black, the coping strategies they used in response to the pandemic, and the ways in which these individuals’ racial and cultural identities impacted their experiences. Such individuals are at unique risk for traumatic stress and burnout. This risk was compounded by the traumatic nature of the dual pandemics and the ways in which they forced confrontation with Yalom's existential givens of human existence: death, isolation, meaninglessness, and freedom and responsibility. Eight client‐facing social workers who identified as Black and lived in the United States were interviewed using a 19‐item semistructured protocol created by the researchers that addressed the research questions. Data were analyzed using Braun and Clarke's thematic analysis methodology. Five themes were constructed: (1) Changes in treatment due to COVID‐19, (2) Emotional toll due to the pandemic, (3) Experiences related to being Black, (4) Beneficial experiences, and (5) Coping with COVID‐19 and ongoing racism. Participants’ experiences demonstrated the compounded psychological risk factors they faced as social workers, Black Americans, and survivors of the pandemic, and the ways in which they confronted existential concerns. Participants demonstrated resilience by making new meanings out of their experiences and implied their successful posttraumatic growth. This study demonstrated the need for greater support of Black social workers in social work practice and policy to ease the compounded stressors they face as a result of the pandemic.
Effects of temperature fluctuations on the quality and microbial diversity of beef meatballs during simulated cold chain distribution
Authors: Yuan Jingjing , Wang Zhaoming , Li Huale , Xu Baocai 
Published: June 2024

AbstractBACKGROUNDCold chain distribution with multiple links maintains low temperatures to ensure the quality of meat products, whereas temperature fluctuations during this are often disregarded by the industry. The present study simulated two distinct temperatures cold chain distribution processes. Quality indicators and high‐throughput sequencing were employed to investigate the effects of temperature fluctuations on the quality and microbial diversity of beef meatballs during cold chain distribution.RESULTSQuality indicators revealed that temperature fluctuations during simulated cold chain distribution significantly (P < 0.05) exacerbated the quality deterioration of beef meatballs. High‐throughput sequencing demonstrated that temperature fluctuations affected the diversity and structure of microbial community. Lower microbial species abundance and higher microbial species diversity were observed in the temperature fluctuations group. Proteobacteria and Pseudomonas were identified as the dominant phylum and genus in beef meatballs, respectively, exhibiting faster growth rates and greater relative abundance under temperature fluctuations.CONCLUSIONThe present study demonstrates that temperature fluctuations during simulated cold chain distribution can worsen spoilage and shorten the shelf life of beef meatballs. It also offers certain insights into the spoilage mechanism and preservation of meat products during cold chain distribution. © 2024 Society of Chemical Industry.
Temporal habitat use of mule deer in the Pueblo of Santa Ana, New Mexico
Authors: Bird Daniel E. , D'Acunto Laura E. , Ginter Daniel , Harper Glenn , Zollner Patrick A. 
Published: June 2024

AbstractMule deer (Odocoileus hemionus) are important economically, culturally, and recreationally to the Pueblo of Santa Ana in central New Mexico, USA. Studies of habitat selection improve our understanding of mule deer ecology in central New Mexico and provide the Tribe with valuable information for management of mule deer. We used global positioning system telemetry‐collar data collected on mule deer around the Pueblo of Santa Ana to create resource selection functions from proximity‐based habitat predictors using a generalized linear mixed model. We created separate resource selection functions for females and males during summer and winter at different times of the day. Season generally had a greater effect on mule deer habitat use than the time of day. Female and male mule deer selected for similar habitats but were sexually segregated in their summer distributions. These findings are consistent with results from other locations where mule deer partitioned habitat similarly between seasons and sexes. Supported models reaffirm accepted patterns of habitat selection for mule deer to the Pueblo of Santa Ana where local results were lacking. Our results can help managers identify locations in and around the Pueblo of Santa Ana where future development such as highway expansion are likely to conflict with mule deer activity and locations where habitat enhancement projects such as adding water sources can have the greatest effect for the deer population.
An Online Mindfulness‐Based Group Intervention for Tics: A Pilot Randomized Controlled Trial
Authors: Reese Hannah E. , Brown William Alan , Summers Berta , Hoeppner Susanne , Miller Sarah B. , Wilhelm Sabine 
Published: June 2024

AbstractBackgroundCurrent treatments for Tourette syndrome (TS) and persistent tic disorder (PTD) are often insufficiently effective, inaccessible, and frequently associated with adverse events. Thus, we must continue to develop and test effective, accessible, and safe treatment options.ObjectiveWe aimed to conduct a pilot randomized controlled trial (RCT) comparing a novel, videoconference‐delivered group mindfulness‐based intervention for tics (MBIT) to videoconference‐delivered group psychoeducation, relaxation, and supportive therapy (PRST) for adults with TS or PTD.MethodsThirty‐two adults with TS or PTD were randomly assigned to receive 8 weeks of either MBIT or PRST. Tic severity, tic‐related impairment, and global improvement were assessed by a trained, independent evaluator who was masked to treatment condition at baseline (week 0), posttreatment (week 9), 1‐month follow‐up, and 6‐month follow‐up. All study procedures were conducted online via secure videoconferencing.ResultsTwenty‐eight participants began treatment and were included in analyses. MBIT, relative to PRST, was associated with a significantly greater decline in tic severity (d = 0.85) and tic‐related impairment (d = 0.99) from baseline to posttreatment. Treatment response was significantly higher in MBIT (69%) than in PRST (13%). Neither treatment resulted in serious adverse effects. The durability of treatment outcomes is also reported and discussed.ConclusionsThe results from this pilot RCT suggest that videoconference‐delivered group MBIT may be an efficacious, accessible, and safe intervention for adults with tics. Future research is necessary to confirm these preliminary findings. © 2024 The Author(s). Movement Disorders published by Wiley Periodicals LLC on behalf of International Parkinson and Movement Disorder Society.
Mutation spectrum of hearing loss patients in Northwest China: Identification of 20 novel variants
Authors: Ma Panpan , Zhou Bingbo , Kang Qichao , Chen Xue , Tian Xinyuan , Hui Ling , Hao Shengju , Wu Huiyan , Zhang Chuan 
Published: June 2024

AbstractBackgroundHearing loss (HL) is the most frequent sensory deficit in humans, with strong genetic heterogeneity. The genetic diagnosis of HL is very important to aid treatment decisions and to provide prognostic information and genetic counselling for the patient's family.MethodsWe detected and analysed 362 Chinese non‐syndromic HL patients by screening of variants in 15 hot spot mutations. Subsequently, 40 patients underwent further whole‐exome sequencing (WES) to determine genetic aetiology. The candidate variants were verified using Sanger sequencing. Twenty‐three carrier couples with pathogenic variants or likely pathogenic variants chose to proceed with prenatal diagnosis using Sanger sequencing.ResultsAmong the 362 HL patients, 102 were assigned a molecular diagnosis with 52 different variants in 22 deafness genes. A total of 41 (11.33%) cases with the biallelic GJB2 (OMIM # 220290) gene mutations were detected, and 21 (5.80%) had biallelic SLC26A4 (OMIM # 605646) mutations. Mitochondrial gene (OMIM # 561000) mutations were detected in seven (1.93%) patients. Twenty of the variants in 15 deafness genes were novel. SOX10 (OMIM # 602229), MYO15A (OMIM # 602666) and WFS1 (OMIM # 606201) were each detected in two patients. Meanwhile, OSBPL2 (OMIM # 606731), RRM2B (OMIM # 604712), OTOG (OMIM # 604487), STRC (OMIM # 606440), PCDH15 (OMIM # 605514), LOXHD1 (OMIM # 613072), CDH23 (OMIM # 605516), TMC1 (OMIM # 606706), CHD7 (OMIM # 608892), DIAPH3 (OMIM # 614567), TBC1D24 (OMIM # 613577), TIMM8A (OMIM # 300356), PTPRQ (OMIM # 603317), SALL1 (OMIM # 602218), and GSDME (OMIM # 608798) were each detected in one patient. In addition, as regards one couple with a heterozygous variant of CDH23 and PCDH15, respectively, prenatal diagnosis results suggest that the foetus had double heterozygous (DH) variants of CDH23 and PCDH15, which has a high risk to cause ID/F type Usher syndrome.ConclusionOur study expanded the spectrum of deafness gene variation, which will contribute to the genetic diagnosis, prenatal diagnosis and the procreation guidance of deaf couple. In addition, the deafness caused by two genes should be paid attention to in the prenatal diagnosis of families with both deaf patients.
Noninvasive prenatal screening in a pregnant woman with a history of stem cell transplant from a male donor: A case report and literature review
Authors: Liang Dong , Lin Ying , Luo Chunyu , Li Hang , Hu Ping , Xu Zhengfeng 
Published: June 2024

AbstractBackgroundAs a screening method, inaccuracies in noninvasive prenatal screening (NIPS) exist, which are often attributable to biological factors. One such factor is the history of transplantation. However, there are still limited reports on such NIPS cases.MethodsWe report an NIPS case of a pregnant woman who had received a stem cell transplant from a male donor. To determine the karyotype in the woman's original cell, we performed chromosome microarray analysis (CMA) on her postnatal blood and oral mucosa. To comprehensively estimate the cell‐free DNA (cfDNA) composition, we further performed standard NIPS procedures on the postnatal plasma. Moreover, we reviewed all published relevant NIPS case reports about pregnant women with transplantation history.ResultsNIPS showed a low‐risk result for common trisomies with a fetal fraction of 65.80%. CMA on maternal white blood cells showed a nonmosaic male karyotype, while the oral mucosa showed a nonmosaic female karyotype. The proportion of donor's cfDNA in postnatal plasma was 94.73% based on the Y‐chromosome reads ratio. The composition of cfDNA in maternal plasma was estimated as follows: prenatally, 13.60% maternal, 65.80% donor, and 20.60% fetal/placental, whereas postnatally, 5.27% maternal and 94.73% donor.ConclusionsThis study expanded our understanding of the influence of stem cell transplantation on NIPS, allowing us to optimize NIPS management for these women.
Kaempferol‐Enhanced Migration and Differentiation of C2C12 Myoblasts via ITG1B/FAK/Paxillin and IGF1R/AKT/mTOR Signaling Pathways
Authors: Hour Tzyh‐Chyuan , Lan Nhi Nguyen Thai , Lai I‐Ju , Chuu Chih‐Pin , Lin Pei‐Chen , Chang Hsi‐Wen , Su Ying‐Fang , Chen Chung‐Hwan , Chen Yu‐Kuei 
Published: June 2024

ScopeKaempferol (KMP), a bioactive flavonoid compound found in fruits and vegetables, contributes to human health in many ways but little is known about its relationship with muscle mass. The effect of KMP on C2C12 myoblast differentiation and the mechanisms that might underlie that effect are studied.Methods and resultsThis study finds that KMP (1, 10 µM) increases the migration and differentiation of C2C12 myoblasts in vitro. Studying the possible mechanism underlying its effect on migration, the study finds that KMP activates Integrin Subunit Beta 1 (ITGB1) in C2C12 myoblasts, increasing p‐FAK (Tyr398) and its downstream cell division cycle 42 (CDC42), a protein previously associated with cell migration. Regarding differentiation, KMP upregulates the expression of myosin heavy chain (MHC) and activates IGF1/AKT/mTOR/P70S6K. Interestingly, pretreatment with an AKT inhibitor (LY294002) and siRNA knockdown of IGF1R leads to a decrease in cell differentiation, suggesting that IGF1/AKT activation is required for KMP to induce C2C12 myoblast differentiation.ConclusionTogether, the findings suggest that KMP enhances the migration and differentiation of C2C12 myoblasts through the ITG1B/FAK/paxillin and IGF1R/AKT/mTOR pathways. Thus, KMP supplementation might potentially be used to prevent or delay age‐related loss of muscle mass and help maintain muscle health.
A size‐dependent energy‐based strain burst criterion
Authors: Verma Rupesh Kumar , Nguyen Giang D. , Karakus Murat , Taheri Abbas 
Published: June 2024

AbstractThis paper presents a size‐dependent energy‐based strain burst criterion linking strength, elasticity, fracture energies and specimen size effect with stress state due to changes in boundary conditions. It proposes the concept of a ‘Burst Envelope’, a surface in three‐dimensional principal stress space derived based on energy storing and dissipation characteristics of a rock sample, taking into account the size of the specimen and potential localised failure pattern. A scalar burst index is also proposed to quantify the bursting scale. To illustrate and verify its functioning, a numerical modelling framework based on the distinct element method and employing a new cohesive‐frictional contact model is used to perform virtual strain burst experiments under different polyaxial loading‐unloading scenarios, mimicking various underground excavation scenarios. The obtained results are in good agreement with the theoretical prediction of burst occurrence. On that basis, the variation of burst possibility and magnitude are investigated with key factors, including confinement level and the material's elastic, strength and fracture properties. The effect of the specimen's aspect ratio and size on the rock burst potential is elaborated and verified using virtual strain burst experiments, facilitating the linking of the proposed theoretical framework with the evaluation of in‐situ strain bursts in rock masses around underground openings.
Effectiveness and patient perspective on the use of intravesical gentamicin instillations to treat recurrent urinary tract infections in neurogenic lower urinary tract dysfunction
Authors: Cameron Anne P. , Castrodad Patricia Maymi , Troost Jonathan , Forchheimer Martin , Tate Denise G. 
Published: June 2024

AbstractIntroductionPatients with neurogenic lower urinary tract dysfunction (NGLUTD) who require catheterization either with clean intermittent catheters (CIC) or indwelling catheters suffer with frequent urinary tract infections (UTIs). This study assessed the efficacy, patient persistence, satisfaction, and the impact on quality of life (QoL) of gentamicin nightly bladder instillations with 15 mg.MethodsThis is a prospective survey of 36 patients with NGLUTD and recurrent UTIs prescribed long‐term gentamicin to prevent UTIs. Eligible patients completed a questionnaire about their use and satisfaction with gentamicin therapy, as well as survey questionnaires to address QoL. A retrospective chart review was also performed to obtain medical history, confirm drug persistence, and obtain accurate UTI data for the 12 months preceding and after starting instillations.ResultsThe rate of laboratory proven symptomatic UTI requiring antibiotic treatment decreased from 3.9 to 1.1 infections per year with no increase in antibiotic resistance and no significant side effects reported by patients. Eight patients stopped therapy before a full year for various reasons, but the remaining 72% of patients have continued to use the therapy now with a mean of 4.2 years later. Satisfaction among those continuing the medication was very high.ConclusionGentamicin bladder instillations with 15 mg nightly in patients with indwelling catheters or CIC with NGLUTD are very effective and safe with high patient satisfaction. This therapy can be maintained long‐term with continued efficacy.
Finite element approximation of stabilized mixed models in finite strain hyperelasticity involving displacements and stresses and/or pressure—An overview of alternatives
Authors: Codina Ramon , Castañar Inocencio , Baiges Joan 
Published: June 2024

AbstractThis paper presents mixed finite element formulations to approximate the hyperelasticity problem using as unknowns the displacements and either stresses or pressure or both. These mixed formulations require either finite element spaces for the unknowns that satisfy the proper inf‐sup conditions to guarantee stability or to employ stabilized finite element formulations that provide freedom for the choice of the interpolating spaces. The latter approach is followed in this work, using the Variational Multiscale concept to derive these formulations. Regarding the tackling of the geometry, we consider both infinitesimal and finite strain problems, considering for the latter both an updated Lagrangian and a total Lagrangian description of the governing equations. The combination of the different geometrical descriptions and the mixed formulations employed provides a good number of alternatives that are all reviewed in this paper.
‘I need support in becoming the leader I would like to be’ – A qualitative descriptive study of nurses newly appointed to positions of leadership
Authors: Skarstein Siv , Lysfjord Else Marie , Silseth Marit Helene , Leegaard Marit 
Published: June 2024

AbstractAimThe aim of the study was to understand the experiences of nurses who were newly appointed to a position of leadership including facilitators and barriers to success and what they considered important for the development of their role.MethodsWe used a qualitative descriptive research design. The study was conducted in Norway with 10 nurses who had been appointed to a leadership position within the last 2 years. Participants were interviewed with individual qualitative interviews which were transcribed and subsequently analysed using thematic analysis.ResultsThe following four main themes were identified from the data: I feel lonely in the leadership position, I am not confident as a leader, I am unsure of the requirements and expectations of me, and I need support and supervision.ConclusionThe results underscore the challenges reported by new leaders in nursing and their advocacy for mentorship as a facilitator for success. The qualifications of mentors were emphasised with a need for a comprehensive leadership skill set to address the multifaceted aspects of leadership development.
Establishing a course to train ICU nurses on prone position mechanical ventilation: A Delphi study
Authors: Jiang Yan , Lu Ye , Ge Jun , Yang Linlin , Wang Dongsheng , Cui Jingping 
Published: June 2024

AbstractAimTo develop a comprehensive training course for training ICU nurses in prone positioning.DesignA mixed study combining semi‐structured interviews and two rounds of Delphi surveys.MethodsWe constructed a questionnaire after collecting data through a literature review and semi‐structured interviews. We used the Delphi expert correspondence method to conduct two rounds of research among 17 experts in the field of critical illness. Data collection took place between May and August 2022.ResultsThe effective questionnaire recovery rate was 88.2%. The expert authority coefficient was 0.876; the Kendall coordination coefficient was 0.402; the average importance score for each index ranged from 4.00 to 4.93; and the coefficient of variation for each index ranged from 0.05 to 0.19. We established 13 second‐level indicators and 41 third‐level indicators on prone position ventilation training according to three aspects: training contents, training methods and training assessment. The training system of prone mechanical ventilation for ICU nurses established in this study will provide an effective framework for training and evaluating the practical ability of prone mechanical ventilation for ICU nurses.