bluuebunny/crossref_metadata_2025_split huggingface dataset:
Craniofacial Injuries in Rugby: A US National Database Analysis
Authors: Suh Cody , Bhasin Garv , Stead Thor S. , Ganti Latha 
Published: February 2025

Introduction:
Rugby is a sport popular in Europe and Australia that resembles American football. A key difference between Rugby and American Football is the lack of pads or protective equipment, which increases the risk of head-to-head collisions and craniofacial injuries. Despite the in-depth research done on head and neck injuries, there is an insufficient amount of research relating to intracranial injuries in rugby. This paper aims to analyze patterns and differences in rugby-related craniofacial injuries.

Materials and Methods:
An analysis of rugby players who sustained facial and neck injuries, including the ear, mouth, and eyes, was performed using the National Electronic Injury Surveillance System (NEISS) data sets from 2014 to 2023. These data sets conveyed patient information such as sex, race, weight, age, diagnosis, treatment date, body part, disposition, and injury location that occurred while playing rugby.

Results:
During the study period, a total of 35,583 craniofacial injuries were estimated to occur in emergency departments across the nation. Among these cases, 27,669 (77.8%) were male, and 7914 (22.2%) were female. The 3 most common injury sites were the head (19,261 cases, 51.4%), face (13,261 cases, 35.4%), and neck (2115 cases, 5.6%). The most frequent diagnoses were lacerations (9906 cases, 25.8%), internal injuries (9543 cases, 24.9%), and concussions (8617 cases, 22.5%).

Conclusion:
In 35,583 total cases, 77.8% of diagnoses were male and 22.2% were female. There has been a downward trajectory in the annual injury rate, going from 5033 cases in 2014 to 2862 cases in 2023. Most diagnoses were internal injuries, lacerations, and concussions. Prevention of and recovery from craniofacial injuries requires greater attention among rugby coaches, players, and fans. Players should be given more time to recover and should consider wearing protective equipment to minimize injury. In addition, youth and amateur players would benefit from targeted education on proper tackling techniques and injury prevention strategies, addressing the heightened vulnerability in younger demographics due to lack of experience and developmental factors.
Resistance to Radiation Enhances Metastasis by Altering RNA Metabolism
Authors: Kumar Ayush , Kishimoto Kensei , Goel Hira Lal , Wisniewski Christi A. , Li Rui , Pacheco Brendan , Zhu Lihua Julie , Flavahan William A. , Mercurio Arthur M 
Published: February 2025

AbstractThe cellular programs that mediate therapy resistance are often important drivers of metastasis, a phenomenon that needs to be understood better to improve screening and treatment options for cancer patients. Although this issue has been studied extensively for chemotherapy, less is known about a causal link between resistance to radiation therapy and metastasis. We investigated this problem in triple-negative breast cancer (TNBC) and established that radiation resistant tumor cells have enhanced metastatic capacity, especially to bone. Resistance to radiation increases the expression of integrin β3 (ITGβ3), which promotes enhanced migration and invasion. Bioinformatic analysis and subsequent experimentation revealed an enrichment of RNA metabolism pathways that stabilize ITGβ3 transcripts. Specifically, the RNA binding protein heterogenous nuclear ribonucleoprotein L (HNRNPL), whose expression is regulated by Nrf2, mediates the formation of circular RNAs (circRNAs) that function as competing endogenous RNAs (ceRNAs) for the family of let-7 microRNAs that target ITGβ3. Collectively, our findings identify a novel mechanism of radiation-induced metastasis that is driven by alterations in RNA metabolism.
Targeting Anti-HLA Class I and II Antibodies with CAR-B Cell Therapy: A Novel Strategy to Mitigate Graft Rejection and Platelet Refractoriness
Authors: Kesherwani Varun 
Published: February 2025

AbstractAlloimmune responses mediated by anti-HLA (human leukocyte antigen) antibodies are significant barriers to successful transplantation and transfusion therapies. Current immunosuppressive strategies target broad components of the immune system, often leading to non-specific effects, such as increased susceptibility to infections and malignancies. Here, we propose a novel therapeutic approach using Chimeric Antigen Receptor (CAR)-B cells engineered to target anti-HLA Class I and II antibodies. By specifically neutralizing these antibodies, CAR-B cell therapy has the potential to mitigate immune responses in allogeneic transplantation and transfusion, reducing the incidence of graft rejection and transfusion reactions. It will also neutralize the T cell response by binding to reactive T cells against donor HLA molecules. In this article, we discuss the rationale, design, and anticipated outcomes of this innovative therapy.
Single-Cell Resolution of Cellular Damage Illuminates Disease Progression
Authors: Padvitski Tsimafei , Unger Avila Paula , Chen He , Özel Cem , Braun Fabian , Müller Roman-Ulrich , Westermark Pål O. , Brinkkötter Paul T. , Schermer Bernhard , Benzing Thomas , Wunderlich F. Thomas , Kann Martin , Beyer Andreas 
Published: February 2025

SummaryDegenerative diseases are marked by the progressive accumulation of cellular damage, leading to impaired cellular function and tissue degeneration. Despite advances in single-cell technologies, capturing the gradual decline of individual cellsin vivoremains challenging. Here, we present a novel, universal, cross-model framework for quantifying cellular damage at single-cell resolution, to uncover conserved molecular trajectories of cellular degeneration. This method uses single-cell RNA sequencing data and enables the detection of progressive damage within distinct cell populations under physiological and pathological conditions. We developed the Podocyte Damage Score (PDS) and Hepatocyte Damage Score (HDS) to monitor cellular deterioration in murine models of kidney glomerulosclerosis and liver steatosis, respectively. The application of these scores to both murine and human datasets accurately quantified cellular damage across diverse disease models and distinguished varying degrees of damage even in unperturbed samples. Notably, the PDS revealed circadian gene expression dysregulation as a hallmark of podocyte injury, while the HDS identified a critical threshold of hepatocyte damage leading to cellular senescence and metabolic dysfunction. The approach provides a scalable tool for decoding disease progression and identifying therapeutic targets across degenerative disorders.
Cryptosporidiummodifies intestinal microvilli through an exported virulence factor
Authors: Rodrigues Elena , Pallett Mitchell A. , Straker Lorian C. , Mkandawire Tapoka T. , Sala Katarzyna , Collinson Lucy , Sateriale Adam 
Published: February 2025

AbstractCryptosporidiumis a common intestinal infection of vertebrates and a significant threat to public health. Within the epithelial layer of the intestine, the parasite invades and replicates. Infected cells are readily detected under the microscope by the presence of elongated microvilli, particularly around the vacuole where the parasite resides. Here, we identify a family ofCryptosporidiumvirulence factors that are exported into the host cell during infection and localise to the microvilli. We examine the trafficking and function of the most highly expressed family member, MVP1, which appears to control the elongation of microvilli through engagement of host EBP50 and CDC42. Remarkably, this mechanism closely mirrors that of an enteropathogenicEscherichia colivirulence factor, MAP, which is also known to drive host microvilli elongation during infection. This highlights a unique instance where eukaryotic and prokaryotic virulence factors have convergently evolved to modulate host actin structures through a similar mechanism.
Crystal structure of a thermophilic family II inorganic pyrophosphatase enabling high-temperature adaptation inThermodesulfobacterium commune
Authors: Maruoka Saki , Teramoto Takamasa , Watanabe Keiichi , Kakuta Yoshimitsu 
Published: February 2025

AbstractInorganic pyrophosphatases (PPases) are crucial for energy metabolism and classified into families with distinct metal ion requirements and structural features. This study is the first to successfully express, purify, and structurally characterize a thermophilic family II PPase isolated fromThermodesulfobacterium commune(TcPPase).TcPPase, which is optimally activated by Co2+and Mn2+, required both the N- and C-terminal domains for complete catalytic function. Comparative structural analyses of psychrophilic and mesophilic homologs revealed that the enhanced thermal stability ofTcPPase was due to its strong hydrophobic interactions, high proline content, dense hydrogen bond network, and additional salt bridges. These findings reveal the molecular basis for the thermal adaptation of family II PPases, providing valuable insights for thermostable enzyme engineering for biotechnological applications.
Small-molecule screen inC. elegansidentifies benzenesulfonamides as inhibitors of microsporidia spores
Authors: Huang Qingyuan , Jiang Haiyi , Wei Junhong , Dou Yabin , Pan Guoqing , Chen Jie , Reinke Aaron W. 
Published: February 2025

AbstractMicrosporidia, a large group of fungal-related intracellular parasites, infect several economically significant animals, leading to substantial economic losses. As currently available anti-microsporidia therapies are either ineffective or come with numerous adverse effects, there is a need for alternative microsporidia inhibitors. Here we screen a subset of the ChemBridge DIVERset library, comprising 2500 diverse compounds, usingCaenorhabditis elegansinfected with its natural microsporidian parasite,Nematocida parisii. By testing these compounds at 60 μM in 96-well assay plates, we identified 26 hits that restored the ability ofC. elegansto produce progeny in the presence ofN. parisii. We confirmed that out of 20 tested compounds, 18 ChemBridge compounds effectively inhibitN. parisiiinfection inC. elegans. Of these 18, 10 were benzenesulfonamide derivatives which inhibit microsporidia infection by inactivating spores. We screened an additional 475-compound benzenesulfonamide library, successfully identifying three compounds that are effective at a lower concentration than the initial hits. We further show that one benzenesulfonamide compound displays inhibitory activity against several species of microsporidia, inhibiting infection of species belonging to theNematocida,Enterocytozoon, andEncephalitozoongenera. Together our results suggest that benzenesulfonamides are a potential scaffold for the development of microsporidia antiseptics.
In toto imaging of germ plasm dynamics reveals an essential role for early distribution of germ granules in germline development
Authors: Zaucker Andreas , Papafoti Maria , Corcoran David , La Rodé DaeNia La Shawn , Leech Rebecca , Vrliczak Pavle , Kumari Pooja , Sampath Karuna 
Published: February 2025

ABSTRACTA fundamental question in developmental biology is how the fertilized egg gives rise to all the different cell types of an organism. The traditional view is that the different cell types are specified either by intrinsic factors such as cell fate determinants or via intercellular signaling. In some animals a cytoplasmic determinant-like substance called ‘germplasm’ specifies the germline. In zebrafish eggs, germplasm is dispersed in form of mRNP complexes called germ granules, which are enriched at the animal pole. After fertilization the distribution of germ granules changes dramatically. The germ granules accumulate in the corners of the first two cleavage furrows of the embryo, to form four large masses that are essential for germline development. Although germ granule movement has been linked to the network dynamics of the microtubular and actin cytoskeleton, a clear mechanistic understanding of the process is currently lacking. Fundamental questions about germplasm dynamics, including “What is the main driving force?” have not been answered yet.To address this gap, we performed careful quantitative analysis of germ granule dynamics relative to dynamic cytoskeletal reorganization in early zebrafish embryos by live-imaging. We identified stereotypic signatures of germ granule dynamics across different regions of the early embryo. Interestingly, we find that the timing of large-scale germ granule movements contrasts prevailing models for the mechanism of germ granule aggregation during cleavage divisions, and rather points to cytokinetic apparatus itself.Using zebrafish mutants affecting the RNA-binding protein Ybx1 (Y-box binding-protein 1), we show that the timing and dynamics of germ granule accumulation in the blastodisc is a crucial factor for appropriate later aggregation into cleavage furrows and eventual distribution to PGCs. Germplasm accumulation in the cleavage furrows is reduced and ectopic aggregates form at the blastoderm margin of ybx1 mutant embryos. Our work establishes Ybx1 as a novel factor with crucial functions in germplasm distribution and suggests that additional factors drive normal germplasm dynamics.
Harnessing artificial intelligence for strategic decision-making: the catalyst impact of digital leadership
Authors: Jaboob Mohammed , Al-Ansi Abdullah M. , Al-Okaily Manaf , Ferasso Marcos 
Published: February 2025

PurposeArtificial intelligence (AI) applications enable entrepreneurs in small and medium-sized enterprises (SMEs) to make strategic decisions based on more accurate predictions and modeling scenarios to achieve operational efficiency and profitability. This research aims to investigate the role of AI applications in enhancing strategic decision-making (SDM) through the mediating role of digital leadership (DL).Design/methodology/approachThe sample included 306 Omani entrepreneurs from SMEs randomly sampled to collect data from different SME industrial incubators. To test hypotheses, structural equation modeling (SEM), regression and confirmatory factor analysis and Process V4 to test the mediating role of DL were used.FindingsResults reveal that AI applications have a positive and significant impact on SDM and DL. Furthermore, DL has a positive and significant impact on SDM. The results also reveal that DL enhances the relationship between AI applications and SDM in Omani SMEs.Originality/valueThis research provides evidence regarding SMEs in the Arabian context and the ability of AI application adoption to improve productivity and sustainability in the economic sector.
Avoiding harm to the product: how product anthropomorphism and buyer usage intent affect sellers’ pricing
Authors: Liu Ping , Li Shouwei , Zhang Lijun , Li Wei 
Published: February 2025

PurposeBuilding on the core concept of anthropomorphism and the empathy-helping theory, this research aims to examine how product anthropomorphism and buyer usage intentions affect sellers’ pricing in second-hand markets as well as explore the psychological dynamics underlying these effects.Design/methodology/approachTo test the hypotheses, a series of four experiments were conducted. Studies 1a (n = 140) and 1b (n = 140) employed a one-factor (product anthropomorphism: yes vs no) between-subject design and used chi-square analysis. Study 2 (n = 145) and Study 3 (n = 162) employed a 2 (usage intention: protective vs destructive) × 2 (product anthropomorphism: yes vs no) between-subject design and used two-way ANOVA and moderated mediation analysis.FindingsThe study found that even when potential buyers with destructive (vs protective) usage intentions offer higher prices, sellers of anthropomorphized (vs non-anthropomorphized) products are less willing to choose them (Studies 1a and 1b). When potential buyers express destructive (vs protective) usage intentions, sellers of anthropomorphized (vs non-anthropomorphized) products are less willing to offer discounts (Study 2), and the lowest price they are willing to accept is higher (Study 3). The level of perceived capacity for pain mediates these effects (Study 3).Originality/valueThese findings offer insights into the application of product anthropomorphism strategies and the second-hand transactions of used anthropomorphized products.
Compressed stabilised earth blocks as a sustainable building material: investigation of customer purchase intentions
Authors: Tissaveerasinghe Stanly Prashanthan , Chathuranga Namila , Siriwardana Chandana 
Published: February 2025

PurposeThis research aims to analyse the influence of multidimensional factors on customer purchasing intentions of compressed stabilised earth blocks (CSEBs), providing relevant authorities with systematic insights to promote CSEBs.Design/methodology/approachA cross-sectional field survey with 222 respondents, preceded by a pilot survey with 30 respondents, was conducted in the Eastern and Northern provinces of Sri Lanka. Descriptive statistics, correlation analysis and multiple linear regression were used to analyse the significance of personal, social, psychological and cultural factors on customer purchase intentions of CSEBs.FindingsBeyond the direct influence of product pricing, factors of lifestyle, social norms/values, perception and social differences significantly shape customer purchasing intentions within the personal, social, psychological and cultural dimensions, respectively. Correlation and multiple linear regression analyses further indicated that personal, social and psychological dimensions substantially influence customer purchasing intentions of CSEBs, while the cultural dimension had negligible influence.Research limitations/implicationsBy identifying key factors shaping customer purchasing intentions, this research lays the groundwork for initiatives aimed at promoting CSEBs. These insights can also inform marketing strategies, educational/awareness programmes and regulatory measures to promote CSEBs effectively.Originality/valueThis research addresses a critical gap in behavioural research on sustainable building materials, focusing on the influence of personal, social, psychological and cultural factors on the customer purchasing intentions of CSEBs. The findings enrich the existing knowledge base and offer novel insights for evidence-based decision-making to promote CSEBs and future behavioural research.
Does corporate governance affect the environmental, social and governance disclosure? A cross-country study
Authors: Mkadmi Jamel Eddine , Daafous Wifak 
Published: February 2025

PurposeThis study aims to explore whether corporate governance mechanisms affect environmental, social and governance (ESG) disclosure by firms across countries. It investigates whether board cultural diversity affects ESG disclosure.Design/methodology/approachThe proposed methodology draws on multidimensional scaling as a multivariate assessment tool to evaluate and prioritize the effect of corporate governance on environmental, social and governance disclosure. This study uses a cross-country sample of 672 listed firms located in 40 countries for the period between 2014 and 2022. We used a panel regression to test the hypotheses. Moreover, we conducted a two-stage least squares regression analysis as an additional robustness check.FindingsThe results show that companies can have high-quality ESG disclosure when they have good corporate governance. Interestingly, this study found that board composition and some criteria of corporate social responsibility (CSR) positively affect ESG disclosure for firms.Originality/valueThis study adds to the existing body of accounting knowledge in several dimensions. Indeed, to the best of our knowledge, this is one of the few studies that investigate the effect of corporate governance on the environmental, social and governance disclosure of firms across 40 countries. This study also has important implications for the board of directors’ characteristics and CSR, which strive to improve the index of ESG disclosure.
Optimization and scheduling of combined heat and power system considering wind power uncertainty and demand response
Authors: Du ShengYi , Tan DongHong , Chen Zitong 
Published: February 2025

Purpose
This study aims to propose a comprehensive optimization and scheduling method for the combined heat and power (CHP) systems that takes into account the uncertainties of wind power and demand response.

Design/methodology/approach
The uncertainty of wind power and the “thermal-electric coupling” characteristics of CHP units have led to an increasing issue of wind power curtailment in CHP systems. With the objective of minimizing the overall scheduling cost of the CHP system, this paper considers the characteristics of interactive loads and wind power uncertainty, and establishes a coordinated optimization scheduling model for the generation-load-storage of the system, based on the inclusion of thermal energy storage devices.

Findings
During the optimization scheduling process, the proposed method in this paper reduces the scheduling cost by ¥99,900 (approximately 36.3%) compared to traditional methods, and significantly decreases the wind power curtailment rate by 53.7%. These results clearly demonstrate the significant advantages of the proposed method in enhancing the economic efficiency of the system and improving wind power integration.

Research limitations/implications
However, the planning process did not take into account the impact of unit combinations and grid structures.

Practical implications
This study proposes a comprehensive optimization and scheduling method for the CHP systems that takes into account the uncertainties of wind power and demand response. The objective function is to minimize the wind curtailment rate’s total scheduling cost, considering the impact of wind power uncertainties and demand response. A coordinated optimization and scheduling model for the generation-load-storage of CHP system is established.

Social implications
CHP units achieve the coupling of electric and thermal energy, significantly improving energy efficiency. In this study, the planning of the CHP system considers the coupling relationships among multiple energy sources, various devices and the pricing optimization spaces of electric and thermal forms of generation, storage and load-side. This approach has achieved favorable results in terms of economic operation scheduling and wind power accommodation improvement.

Originality/value
The case method is used to handle the uncertainty of wind power output on the generation side. Demand response is integrated on the load side to adjust user load curves. On the storage side, the thermal-electric coupling constraints of the CHP units are decoupled using thermal energy storage devices, while considering the economic benefits of all three parties involved: the power source, the load and the energy storage.