bluuebunny/crossref_metadata_2025_split huggingface dataset:
The homeobox gene MSX2 determines chemosensitivity of pancreatic cancer cells via the regulation of transporter gene ABCG2
Authors: Hamada Shin , Satoh Kennichi , Hirota Morihisa , Kanno Atsushi , Umino Jun , Ito Hiromichi , Masamune Atsushi , Kikuta Kazuhiro , Kume Kiyoshi , Shimosegawa Tooru 
Published: April 2011

AbstractPancreatic cancer is one of the life‐threatening cancers due to the difficulty in the curative surgery and resistance against conventional therapeutic strategies. Recent studies indicated that cancer stem cells, which exist as a small number of cells within the entire cancer tissue, contribute to the disease progression. Cancer stem cells reveal resistance against conventional chemotherapy, which is derived from the high‐expression of multiple transporter genes. Our previous study demonstrated the aggravating role of the homeobox gene MSX2 as an inducer of epithelial‐mesenchymal transition, and MSX2 turned out to correlate with the chemoresistance in the current study. Comprehensive analysis of the MSX2‐target gene has identified ABCG2 as the responsible gene. Since previous studies reported the pivotal role of ABCG2 as a determining factor of cancer stem cells, the detailed regulatory mechanism of ABCG2 expression by MSX2 was investigated. As a result, the MSX2 expression level in each cell line well correlated with the ABCG2 expression level, and alteration of the MSX2 expression level by over‐expression or siRNA‐based knockdown affected the ABCG2 expression accordingly. Finally, we identified the functional cooperation of MSX2 and SP1 in the transcriptional regulation of ABCG2 via the SP1 binding elements within the ABCG2 promoter. These findings clarified the intriguing regulatory mechanism of the cancer stem cell‐related gene, and will delineate a novel therapeutic target in pancreatic cancer. J. Cell. Physiol. 227: 729–738, 2012. © 2011 Wiley Periodicals, Inc.
Insulin‐like growth factor 1 stimulation of androgen receptor activity requires β1A integrins
Authors: Sayeed Aejaz , Alam Naved , Trerotola Marco , Languino Lucia R. 
Published: April 2011

AbstractDespite the findings that β1 integrins play a vital role in the regulation of cell proliferation and survival, the mechanisms through which they operate and lead to cancer progression remain elusive. Previously, our laboratory has shown that β1A integrins support insulin‐like growth factor 1 (IGFI)‐mediated mitogenic and transforming activities. Here, we report that β1A integrins regulate basal levels of IGF‐IR, although they are not critical for maintaining cancer cell morphology. Upon transfection of β1A siRNA and consequent downregulation of IGF‐IR, we show inhibition of anchorage‐independent growth of prostate cancer cells, a function which is dependent on IGF‐IR expression. In addition, we demonstrate that IGFI‐mediated activation of androgen receptor (AR), known to occur in prostate cancer cells, requires expression of β1A integrins as evaluated by luciferase reporter assays and immunoblotting analysis. Since β1A integrin levels are increased by R1881 or dihydrotestosterone (DHT), our results imply that β1A integrins support an androgen‐enhanced feedback loop that regulates the expression of IGF‐IR. β1A integrins also regulate inducible levels of IGF‐IR in cells stimulated by androgen or by a combination of androgen and IGFI, as evaluated by flow cytometric analysis and immunoblotting. Furthermore, upon transfection of β1A siRNA and consequent downregulation of IGF‐IR, neither activation of AKT, an effector of IGF‐IR, nor AR levels are affected. We conclude that β1A integrin expression is critical for maintaining the regulatory crosstalk between IGF‐IR and AR. J. Cell. Physiol. 227: 751–758, 2012. © 2011 Wiley Periodicals, Inc.
The switch from pRb/p105 to Rb2/p130 in DNA damage and cellular senescence
Authors: Helmbold Heike , Galderisi Umberto , Bohn Wolfgang 
Published: April 2011

AbstractCellular senescence is a response to genotoxic stress that results in an irreversible cell cycle arrest. Activation of this pathway relies on the activity of the retinoblastoma proteins and proteins of the DNA damage response cascade. Here, we discuss the functional relevance of the switch from pRb/p105 to Rb2/p130 that becomes apparent when cells enter senescent arrest. J. Cell. Physiol. 227: 508–513, 2012. © 2011 Wiley Periodicals, Inc.
Seawater at the nanoscale: marine gel imaged by atomic force microscopy
Authors: Radić Tea Mišić , Svetličić Vesna , Žutić Vera , Boulgaropoulos Beate 
Published: April 2011

AbstractThe present study introduces atomic force microscopy (AFM) as a tool for characterization of marine gel network and marine biopolymers self‐assembly, not accessible by other techniques. AFM imaging of marine gel samples collected in summers 2003 and 2004 in the northern Adriatic Sea provided insight into molecular organization of gel network and associations between polysaccharide fibrils in the network. Initial stages of biopolymers self‐assembly were visualized by AFM in a phytoplankton bloom experiment performed in the same aquatorium. Based on AFM imaging and differential scanning calorimetry, the marine gel is characterized as a thermoreversible physical gel and the dominant mode of gelation as crosslinking of polysaccharide fibrils by hydrogen bonding which results in helical structures and their associations. Direct deposition of whole seawater on freshly cleaved mica followed by rinsing was the procedure that caused the least impact on the original structures of biopolymer assemblies in seawater. Copyright © 2011 John Wiley & Sons, Ltd.
Unraveling lipid/protein interaction in model lipid bilayers by Atomic Force Microscopy
Authors: Alessandrini Andrea , Facci Paolo 
Published: April 2011

AbstractThe current view of the biological membrane is that in which lipids and proteins mutually interact to accomplish membrane functions. The lateral heterogeneity of the lipid bilayer can induce partitioning of membrane‐associated proteins, favoring protein–protein interaction and influence signaling and trafficking. The Atomic Force Microscope allows to study the localization of membrane‐associated proteins with respect to the lipid organization at the single molecule level and without the need for fluorescence staining. These features make AFM a technique of choice to study lipid/protein interactions in model systems or native membranes. Here we will review the technical aspects inherent to and the main results obtained by AFM in the study of protein partitioning in lipid domains concentrating in particular on GPI‐anchored proteins, lipidated proteins, and transmembrane proteins. Whenever possible, we will also discuss the functional consequences of what has been imaged by Atomic Force Microscopy. Copyright © 2011 John Wiley & Sons, Ltd.
Nuclear delivery mechanism of herpes simplex virus type 1 genome
Authors: Liashkovich Ivan , Hafezi Wali , Kühn Joachim M , Oberleithner Hans , Shahin Victor 
Published: April 2011

AbstractHerpes simplex virus type 1 (HSV‐1) is a widespread human pathogen infecting more than 80% of the population worldwide. Its replication involves an essential, poorly understood multistep process, referred to as uncoating. Uncoating steps are as follows: (1) The incoming capsid pinpoints the nuclear pore complex (NPC). (2) It opens up at the NPC and releases the highly pressurized viral genome. (3) The viral genome translocates through the NPC. In the present review, we highlight recent advances in this field and propose mechanisms underlying the individual steps of uncoating. We presume that the incoming HSV‐1 capsid pinpoints the NPC by hydrophobic interactions and opens up upon binding to NPC proteins. Genome translocation is initially pressure‐driven. Copyright © 2011 John Wiley & Sons, Ltd.
Intragastric approach for submucosal tumors located near the Z‐line: A hybrid laparoscopic and endoscopic technique
Authors: Shim Jung Ho , Lee Han Hong , Yoo Han Mo , Jeon Hae Myung , Park Cho Hyun , Kim Jun Gi , Song Kyo Young 
Published: April 2011

AbstractBackgroundThe present study was designed to evaluate the feasibility and impact of the “intragastric” approach to laparoscopic wedge resection as a surgical option for the treatment of suspected small sized gastric submucosal tumors (SMTs) located at the level of Z‐line.MethodsWe reviewed six patients who underwent laparoscopic intragastric resection of a suspected gastric SMT. In all cases, tumor mass was located just below the Z‐line. The patients' clinicopathologic characteristics and surgical outcomes were prospectively recorded and reviewed.ResultsAll six patients were underwent successfully laparoscopic wedge resection by the intragastric approach using a balloon‐type trocar. No case was required open conversion. The mean operation time was 128 min, and the mean hospital stay was 4 days. Five cases were proven to have leiomyoma, and one case was found to have gastrointestinal stromal tumor with low risk. The mean tumor size was 2.7 cm.ConclusionLaparoscopic resection through the “intragastric” approach is a safe and feasible option for gastric SMTs located at the level of Z‐line. J. Surg. Oncol. 2011; 104:312–315. © 2011 Wiley‐Liss, Inc.
A COSMO‐RS based QSPR model for the lubricity of biodiesel and petrodiesel components
Authors: Masuch K. , Fatemi A. , Murrenhoff H. , Leonhard K. 
Published: April 2011

ABSTRACTThe development of a new, optimised fuel is a process in which various necessary fuel properties have to be taken into account. For an inclusion of candidates not synthesised yet into the design process, a fully predictive model relying on nothing but the molecular structure is mandatory for each relevant property. One of the most important aspects for the design of a fuel is its lubricity. In this study, the predictive method conductor‐like screening model for realistic solvation (COSMO‐RS) for the calculation of thermodynamic mixture properties is adopted for deriving a quantitative structure–property relationship for the lubricity of fuel components. COSMO‐RS calculates molecular descriptors (sigma moments) based on quantum chemical calculations. These descriptors are adapted to describe the underlying phenomena causing the film formation ability and lubricity of the fuel. The lubricity is assessed via high‐frequency reciprocating rig measurements taken from literature. The molecular descriptors and experimental data are evaluated via statistical methods in order to find the most influential molecular descriptors. Copyright © 2011 John Wiley & Sons, Ltd.
Human papillomavirus vaccine: Widening the scope for cancer prevention
Authors: No Jae Hong , Kim Mi‐Kyung , Jeon Yong‐Tark , Kim Yong‐Beom , Song Yong‐Sang 
Published: April 2011

AbstractHuman papillomavirus (HPV) is the necessary cause of cervical cancer. The HPV oncoproteins E6 and E7 have crucial roles in various steps of carcinogenesis, inducing degradation of p53 and destabilization of pRb. Several clinical trials show that recombinant HPV vaccines are safe and effective in preventing persistent infection of HPV and associated anogenital lesions. Although most clinical studies to date have investigated the effectiveness of HPV vaccines in young female subjects, elderly females and males may also be candidates for HPV vaccines. Prophylactic HPV vaccination may be an ideal preventive method for other HPV‐associated cancers in addition to cervical carcinoma. Carcinogenesis by HPV, efficacy trials of currently available HPV vaccines, and the possible roles of HPV vaccines in the prevention of HPV‐associated cancers are reviewed in this article. © 2011 Wiley‐Liss, Inc.
Redox modulation of p53: Mechanisms and functional significance
Authors: Kim Do‐Hee , Kundu Joydeb Kumar , Surh Young‐Joon 
Published: April 2011

AbstractThe tumor suppressor protein p53 functions as a stress‐responsive transcription factor. In response to oxidative, nitrosative, and electrophilic insults, p53 undergoes post‐translational modifications, such as oxidation and covalent modification of cysteines, nitration of tyrosines, acetylation of lysines, phosphorylation of serine/threonine residues, etc. Because p53 plays a vital role in the transcriptional regulation of genes encoding proteins involved in a wide spectrum of biochemical processes including DNA repair, cell‐cycle regulation, and programmed cell death, the redox‐modification of p53 appears to be an important determinant of cell fate. This review highlights the redox regulation of p53 and its consequences on cellular function. © 2011 Wiley‐Liss, Inc.
Antioxidant‐rich coffee reduces DNA damage, elevates glutathione status and contributes to weight control: Results from an intervention study
Authors: Bakuradze Tamara , Boehm Nadine , Janzowski Christine , Lang Roman , Hofmann Thomas , Stockis Jean‐Pierre , Albert Franz W. , Stiebitz Herbert , Bytof Gerhard , Lantz Ingo , Baum Matthias , Eisenbrand Gerhard 
Published: April 2011

AbstractEpidemiological and experimental evidence increasingly suggests coffee consumption to be correlated to prevention or delay of degenerative diseases connected with oxidative cellular stress. In an intervention study comprising 33 healthy volunteers, we examined DNA‐protective and antioxidative effects exerted in vivo by daily ingestion of 750 mL of freshly brewed coffee rich in both green coffee bean constituents as well as roast products. The study design encompassed an initial 4 wk of wash‐out, followed by 4 wk of coffee intake and 4 wk of second wash‐out. At the start and after each study phase blood samples were taken to monitor biomarkers of oxidative stress response. In addition, body weight/composition and intake of energy/nutrients were recorded. In the coffee ingestion period, the primary endpoint, oxidative DNA damage as measured by the Comet assay (±FPG), was markedly reduced (p<0.001). Glutathione level (p<0.05) and GSR‐activity (p<0.01) were elevated. Body weight (p<0.01)/body fat (p<0.05) and energy (p<0.001)/nutrient (p<0.001–0.05) intake were reduced. Our results allow to conclude that daily consumption of 3–4 cups of brew from a special Arabica coffee exerts health beneficial effects, as evidenced by reduced oxidative damage, body fat mass and energy/nutrient uptake.
Morphology enabled dipole inversion (MEDI) from a single‐angle acquisition: Comparison with COSMOS in human brain imaging
Authors: Liu Tian , Liu Jing , de Rochefort Ludovic , Spincemaille Pascal , Khalidov Ildar , Ledoux James Robert , Wang Yi 
Published: April 2011

AbstractMagnetic susceptibility varies among brain structures and provides insights into the chemical and molecular composition of brain tissues. However, the determination of an arbitrary susceptibility distribution from the measured MR signal phase is a challenging, ill‐conditioned inverse problem. Although a previous method named calculation of susceptibility through multiple orientation sampling (COSMOS) has solved this inverse problem both theoretically and experimentally using multiple angle acquisitions, it is often impractical to carry out on human subjects. Recently, the feasibility of calculating the brain susceptibility distribution from a single‐angle acquisition was demonstrated using morphology enabled dipole inversion (MEDI). In this study, we further improved the original MEDI method by sparsifying the edges in the quantitative susceptibility map that do not have a corresponding edge in the magnitude image. Quantitative susceptibility maps generated by the improved MEDI were compared qualitatively and quantitatively with those generated by calculation of susceptibility through multiple orientation sampling. The results show a high degree of agreement between MEDI and calculation of susceptibility through multiple orientation sampling, and the practicality of MEDI allows many potential clinical applications. Magn Reson Med, 2011. © 2011 Wiley‐Liss, Inc.
Permeability dependence study of the focused ultrasound‐induced blood–brain barrier opening at distinct pressures and microbubble diameters using DCE‐MRI
Authors: Vlachos Fotios , Tung Yao‐Sheng , Konofagou Elisa 
Published: April 2011

AbstractBlood–brain barrier opening using focused ultrasound and microbubbles has been experimentally established as a noninvasive and localized brain drug delivery technique. In this study, the permeability of the opening is assessed in the murine hippocampus after the application of focused ultrasound at three different acoustic pressures and microbubble sizes. Using dynamic contrast‐enhanced MRI, the transfer rates were estimated, yielding permeability maps and quantitative Ktrans values for a predefined region of interest. The volume of blood–brain barrier opening according to the Ktrans maps was proportional to both the pressure and the microbubble diameter. A Ktrans plateau of ∼0.05 min−1 was reached at higher pressures (0.45 and 0.60 MPa) for the larger sized bubbles (4–5 and 6–8 μm), which was on the same order as the Ktrans of the epicranial muscle (no barrier). Smaller bubbles (1–2 μm) yielded significantly lower permeability values. A small percentage (7.5%) of mice showed signs of damage under histological examination, but no correlation with permeability was established. The assessment of the blood–brain barrier permeability properties and their dependence on both the pressure and the microbubble diameter suggests that Ktrans maps may constitute an in vivo tool for the quantification of the efficacy of the focused ultrasound‐induced blood–brain barrier opening. Magn Reson Med, 2011. © 2011 Wiley‐Liss, Inc.
Issues of discontinuity in the impulse residue function for deconvolution analysis of dynamic contrast‐enhanced MRI data
Authors: Koh T. S. , Cheong D. L. H. , Hou Z. 
Published: April 2011

AbstractRecent dynamic contrast‐enhanced MRI studies using the adiabatic tissue homogeneity model have highlighted potential issues of difficulty in convergence during data‐fitting and reduced parameter precision, due to discontinuities in the adiabatic tissue homogeneity model. This study presents two solutions (an analytic approach and a discrete correction method) to such convergence problems and show that these problems can be attributed to an inaccurate approximation of the convolution integral based on the standard trapezoidal quadrature. It is further explained that such issues of discontinuity in the impulse residue function do not pertain only to the adiabatic tissue homogeneity model, but are generic to all tracer kinetic models, if the difference in bolus arrival time between the arterial input and tissue voxels were to be accounted for simultaneously during model‐fitting. Magn Reson Med, 2011. © 2011 Wiley‐Liss, Inc.
Computationally rapid method of estimating signal‐to‐noise ratio for phased array image reconstructions
Authors: Wiens Curtis N. , Kisch Shawn J. , Willig‐Onwuachi Jacob D. , McKenzie Charles A. 
Published: April 2011

AbstractMeasuring signal‐to‐noise ratio (SNR) for parallel MRI reconstructions is difficult due to spatially dependent noise amplification. Existing approaches for measuring parallel MRI SNR are limited because they are not applicable to all reconstructions, require significant computation time, or rely on repeated image acquisitions. A new SNR estimation approach is proposed, a hybrid of the repeated image acquisitions method detailed in the National Electrical Manufacturers Association (NEMA) standard and the Monte Carlo based pseudo‐multiple replica method, in which the difference between images reconstructed from the unaltered acquired data and that same data reconstructed after the addition of calibrated pseudo‐noise is used to estimate the noise in the parallel MRI image reconstruction. This new noise estimation method can be used to rapidly compute the pixel‐wise SNR of the image generated from any parallel MRI reconstruction of a single acquisition. SNR maps calculated with the new method are validated against existing SNR calculation techniques. Magn Reson Med, 2011. © 2011 Wiley‐Liss, Inc.
Spatially 2D‐selective RF excitations using the PROPELLER trajectory: Basic principles and application to MR spectroscopy of irregularly shaped single voxel
Authors: Busch Martin G. , Finsterbusch Jürgen 
Published: April 2011

AbstractSpatially two‐dimensional selective radio frequency (2DRF) excitations are able to excite arbitrarily‐shaped profiles in their excitation plane and, hence, can be used to minimize partial volume effects in single‐voxel magnetic resonance spectroscopy. In this study, 2DRF excitations based on the PROPELLER trajectory which consists of blades of parallel lines that are rotated against each other, are presented. Because the k‐space center is covered with each segment, the trajectory yields a high signal efficiency which, e.g., is considerably improved compared to a segmented blipped‐planar approach. It is shown that a sampling density correction based on the PROPELLER trajectory's Voronoi diagram suppresses unwanted side excitations. Off‐resonance effects like chemical‐shift displacement artifacts, can be minimized by applying nonselective refocusing radio frequency pulses between the lines of a blade. With half‐Fourier segments, the 2DRF's echo time contribution can be shortened considerably. Thus, robust 2DRF excitations capable of exciting high‐resolution profiles at short echo times with high signal efficiency are obtained. Their applicability to MR spectroscopy of an arbitrarily‐shaped single voxel is demonstrated in a two‐bottle phantom and in the human brain in vivo on a 3 T whole‐body MR system. Magn Reson Med, 2011. © 2011 Wiley Periodicals, Inc.
Sodium and chloride channelopathies with myositis: Coincidence or connection?
Authors: Matthews Emma , Miller James A.L. , MacLeod Malcolm R. , Ironside James , Ambler Gareth , Labrum Robin , Sud Richa , Holton Janice L. , Hanna Michael G. 
Published: April 2011

AbstractIntroduction: A proximal myopathy develops in some patients with muscle channelopathies, but the causative molecular mechanisms are unknown. Methods: We reviewed retrospectively all clinical and muscle biopsy findings of 3 patients with channelopathy and additional myositis. Direct DNA sequencing was performed. Results: Pathogenic mutations were identified in each case. Biopsies demonstrated inflammatory infiltrates. Conclusions: Clinicians should consider muscle biopsy in channelopathy patients with severe myalgia and/or subacute weakness and accompanying elevated creatine kinase. Chance association of myositis and channelopathy is statistically unlikely. An alternative hypothesis suggests that inflammatory insults could contribute to myopathy in some patients. Muscle Nerve, 2011
Reliability of thermal quantitative sensory testing of the hand in a cohort of young, healthy adults
Authors: Moloney Niamh A. , Hall Toby M. , O'Sullivan Tomas C. , Doody Catherine M. 
Published: April 2011

AbstractIntroduction: The reliability of thermal quantitative sensory testing (QST) has yet to be fully established. In this study we investigated intra‐ and interrater reliability of thermal QST in a blinded manner. Methods: Two investigators recorded thermal detection and pain thresholds on the hand of 22 volunteers, twice on two occasions. Results were analyzed using descriptive statistics, intraclass correlation coefficients (ICCs), and coefficients of variation (CVs). Results: Mean intraindividual differences were small for all measures except cold pain thresholds. ICC values for intra‐ and interrater reliability were: cold detection, 0.27–0.55; warm detection, 0.33–0.69; and heat pain, 0.39–0.86. Cold pain yielded high ICC values (0.87–0.94), but also high CV (84.9–90.2%). Conclusions: In young, healthy adults, thermal detection and heat pain thresholds of the hand demonstrated good reliability for group comparisons and individual analyses. Cold pain threshold measures may be suitable for group comparisons, but a large variance in the data limits individual analyses. Muscle Nerve, 2011