bluuebunny/crossref_metadata_2025_split huggingface dataset:
Value of Adrenal Venous Sampling for Lesion Localization in Primary Aldosteronism
Authors: Oh Eun Mee , Lee Kyu Eun , Yoon Kwan , Kim Seong Yeon , Kim Hyo‐Cheol , Youn Yeo‐Kyu 
Published: June 2012

AbstractBackgroundDistinguishing between unilateral and bilateral adrenal lesions is mandatory for surgical treatment of primary aldosteronism (PA). Adrenal venous sampling (AVS) is considered the gold standard for identification and localization of the lesion or lesions causing PA. The objective of the present study was to determine the usefulness of AVS in PA patients.Patients and methodsFrom January 2001 to October 2011, 86 patients with the biochemical diagnosis of PA were retrospectively analyzed. The study group included 45 males and 41 females with a mean age of 50.7 ± 12.6 years, and all patients underwent adrenal computed tomography (CT) and AVS.ResultsThe catheterization success rate of AVS was 82.69 % (86/104). In addition, AVS revealed bilateral lesions in 15/75 patients with unilateral abnormalities diagnosed by CT. These patients underwent medical treatment instead of surgery. One patient had an adrenal mass on the right side, but AVS localized the lesion on the left side. This patient underwent left adrenalectomy. Furthermore, AVS revealed a unilateral lesion in 2/5 patients with bilateral abnormalities demonstrated by CT. These patients underwent unilateral adrenalectomy. Finally, AVS demonstrated localization in 1/6 of patients with no CT abnormalities who were subjected to surgery. Fifty‐three patients with unilateral lesion and one patient with bilateral hypersecretion underwent surgical removal of the affected gland(s). All patients had resolution of hypokalemia and clinical improvement of hypertension.ConclusionsMany patients (19/86, or 22.09 %) would have been inappropriately managed if decision making had been based solely on CT findings. Therefore, AVS is recommended before determining definitive PA management.
Efficient feeding profile optimization for recombinant protein production using physiological information
Authors: Wechselberger Patrick , Sagmeister Patrick , Engelking Helge , Schmidt Torsten , Wenger Jana , Herwig Christoph 
Published: June 2012

Abstract
A multivariate study was performed aiming at the optimization of a recombinant rhamnose inducible E. coli induction system with alkaline phosphatase as target product. The effects of typical factors with impact on post- as well as pre-induction feeding rates were investigated with respect to the space–time yield of the target product. The goal was increased understanding as well as quantitative characterization of these factors with respect to their physiological impact on the model system. The optical density (OD) at which the culture was induced had a strong positive effect on the space–time yield. Pre-induction growth rate (k) had a second-order effect, while induction feed rate drop (J), a factor defining the linear post-induction feed rate, was interacting with (k). However, explanation of the observed effects to acquire more understanding regarding their effect on cell metabolism was not straight forward. Hence, the original process parameters were transformed into physiological more meaningful parameters and served as the basis for a multivariate data analysis. The observed variance with respect to observed volumetric activity was fully explained by the specific substrate uptake rate (q
s) and induction OD, merging the process parameters pre-induction growth rate (k) and feed rate drop (J) into the physiological parameter specific substrate uptake rate (q
s). After transformation of the response volumetric activity (U/ml) into the biomass specific activity (U/gbiomass), the observed variance was fully explained solely by the specific substrate uptake rate (q
s). Due to physiological multivariate data analysis, the interpretation of the results was facilitated and factors were reduced. On the basis of the obtained results, it was concluded that the physiological parameter q
s rather than process parameters (k, J, induction OD) should be used for process optimization with respect to the feeding profile.
The Evaluation of Arkansas Act 1220 of 2003 to Reduce Childhood Obesity: Conceptualization, Design, and Special Challenges
Authors: Phillips Martha M. , Raczynski James M. , West Delia S. , Pulley LeaVonne , Bursac Zoran , Leviton Laura C. 
Published: June 2012

AbstractThis article describes the evaluation of the Arkansas Act 1220 of 2003, a comprehensive legislative proposal to address the growing epidemic of childhood obesity through changes in the school environment. In addition, the article discusses specific components of the evaluation that may be applicable to other childhood obesity policy evaluation efforts. The conceptual framework for the evaluation, research questions, and evaluation design are described, along with data collection methods and analysis strategies. A mixed methods approach, including both quantitative (surveys, telephone interviews) and qualitative (key informant interviews, records reviews) approaches, was utilized to collect data from a range of informant groups including parents, adolescents, school principals, school district superintendents, and other stakeholders. Challenges encountered with the evaluation are discussed, as are strategies to overcome those challenges. Now in its 9th year, this evaluation has documented substantial changes to school policies and environments but fewer changes to student and family behaviors. The evaluation may inform the methods of other evaluations of childhood obesity prevention policies, as well as inform policymakers about how quickly they might expect implementation of such policies in their own states and localities and anticipate both positive and adverse outcomes.
Thirty years beyond discovery—Clinical trials in succinic semialdehyde dehydrogenase deficiency, a disorder of GABA metabolism
Authors: Vogel Kara R. , Pearl Phillip L. , Theodore William H. , McCarter Robert C. , Jakobs Cornelis , Gibson K. Michael 
Published: June 2012

AbstractThis review summarizes a presentation made at the retirement Symposium of Prof. Dr. Cornelis Jakobs in November of 2011, highlighting the progress toward clinical trials in succinic semialdehyde dehydrogenase (SSADH) deficiency, a disorder first recognized in 1981. Active and potential clinical interventions, including vigabatrin, L‐cycloserine, the GHB receptor antagonist NCS‐382, and the ketogenic diet, are discussed. Several biomarkers to gauge clinical efficacy have been identified, including cerebrospinal fluid metabolites, neuropsychiatric testing, MRI, EEG, and measures of GABAergic function including (11 C)flumazenil positron emission tomography (PET) and transcranial magnetic stimulation (TMS). Thirty years after its discovery, encompassing extensive studies in both patients and the corresponding murine model, we are now running an open‐label trial of taurine intervention, and are poised to undertake a phase II trial of the GABAB receptor antagonist SGS742.
UNIVERSITY STUDENTS' KNOWLEDGE OF, AND ATTITUDES TOWARDS, HIV AND AIDS, HOMOSEXUALITY AND SEXUAL RISK BEHAVIOUR: A QUESTIONNAIRE SURVEY IN TWO FINNISH UNIVERSITIES
Authors: KORHONEN TEIJA , KYLMÄ JARI , HOUTSONEN JARMO , VÄLIMÄKI MARITTA , SUOMINEN TARJA 
Published: June 2012

SummaryThis study describes Finnish university students' knowledge and attitudes towards HIV and AIDS, homosexuality and sexual risk behaviour. Finnish-speaking students were randomly selected from all registered students at two universities in Finland (N=9715, n=950). The data were collected by using a modified version of the State University of New York at Buffalo School of Nursing AIDS Study Questionnaire on sexual risk behaviour developed by Held and Chng. The total response rate was 35% (n=333). The data were analysed using quantitative statistical methods. Normally distributed data were analysed by t-test and one-way ANOVA, with Bonferroni corrections. Non-normally distributed data were analysed using the Mann-Whitney U-test and Kruskal-Wallis test, followed by a post-hoc test. The majority of students were familiar with HIV and AIDS, including its mode of transmission. However, there were still some misconceptions concerning HIV and AIDS. The oldest students and women had a more positive attitude towards people living with HIV and AIDS (PLWHA). Of patients with HIV or AIDS, intravenous drug users were perceived most negatively. Male students had more homophobic attitudes. Students who reported that religion had an important role in their lives had significantly stricter attitudes towards sexual risk behaviour. Students' knowledge correlated positively with general attitudes towards HIV and AIDS. Knowledge about HIV and AIDS will lead to more positive attitudes towards HIV and AIDS as a disease, towards those infected as well as homosexual people. There is a need to focus on preventive health care and sexual health promotion by educating young people and changing their attitudes towards sexual risk behaviour.
Direct observations of microscale turbulence and thermohaline structure in the Kuroshio Front
Authors: Nagai Takeyoshi , Tandon Amit , Yamazaki Hidekatsu , Doubell Mark J. , Gallager Scott 
Published: June 2012

Direct observations of microstructure near the Kuroshio Front were conducted in August 2008 and October 2009. These show negative potential vorticity (PV) in the mixed layer south of the front, where directly measured turbulent kinetic energy dissipation rates are an order magnitude larger than predicted by wind‐scaling. These elevated dissipation rates scale better with an empirical scaling, which considers local wind and Ekman buoyancy flux driven by downfront wind. Near‐zero PV in the thermocline under the Kuroshio mainstream is observed at 200–300 m depth, with dissipation exceeding open ocean thermocline values by factors of 10–100. Overall, the large turbulent dissipation rates measured in the Kuroshio can be categorized into two groups, one characterized by low Richardson number along the Kuroshio Front thermocline, and the other characterized by high stratification away from the Kuroshio mainstream. The former is attributed to mixing by unbalanced frontal ageostrophic flows, and the latter is attributed to internal wave breaking. On average, both groups appear in regions of large horizontal density gradients. Observed thermohaline structure shows low salinity tongues from the surface to over 300 m depth and deep cold tongues, extending upward from 500 to 100 m depth in a narrow (20 km) zone, suggesting down and upwelling driven by geostrophic straining, which is confirmed by Quasigeostrophic‐Omega equation solutions. This implies that adiabatic along isopycnal subduction and diabatic diapycnal turbulent mixing acting in tandem at the Kuroshio Front likely contribute to NPIW formation.
Age and Growth of Atlantic Sturgeon in the James River, Virginia, 1997–2011
Authors: Balazik Matthew T. , McIninch Stephen P. , Garman Greg C. , Latour Robert J. 
Published: June 2012

AbstractHistorically the Chesapeake Bay supported a large population of Atlantic sturgeonAcipenser oxyrinchus, but loss of suitable spawning habitat and overfishing coincided with dramatic in‐system declines throughout the 20th century. Atlantic sturgeon harvest moratoriums were implemented in 1974 for Virginia waters and were expanded coastwide in 1998. In 1997, researchers became aware that commercial fishers in the James River, a tributary of the Chesapeake Bay, were catching juvenile and subadult Atlantic sturgeon as bycatch in various fisheries. Genetic studies showed that the Chesapeake Bay population has maintained genetic integrity and qualifies as a distinct population segment. Between 2007 and 2011, almost 150 adults have been caught in the tidal–freshwater portion of the James River during putative spawning runs. Pectoral fin spines from juveniles and subadults collected in the Burwell Bay (rkm 40) and Cobham Bay (rkm 60) areas and mature adult samples from vessel strikes in freshwater around or above rkm 120 were analyzed to create a length‐at‐age curve for Atlantic sturgeon in the James River. Five models were used to analyze the data, and the double von Bertalanffy (k1= 0.054,k2= 0.097,t1= −2.85,t2= 1.09,tp= 6.03 years,L∞= 2241 mm) provided the best fit to the observed data. We estimated an increase in growth coefficient attp, which could be an artifact of low sample size or due to ontogenetic changes in habitat use as older fish spend more time in oceanic waters than younger fish. Atlantic sturgeon in the 6–9 year age range are rarely encountered in the James River compared with younger and older age‐classes, so a more in‐depth analysis of the increased growth coefficient would require ocean sampling.
Brook Trout Movement in Response to Temperature, Flow, and Thermal Refugia within a Complex Appalachian Riverscape
Authors: Petty J. Todd , Hansbarger Jeff L. , Huntsman Brock M. , Mazik Patricia M. 
Published: June 2012

AbstractWe quantified movements of brook trout Salvelinus fontinalis and brown trout Salmo trutta in a complex riverscape characterized by a large, open‐canopy main stem and a small, closed‐canopy tributary in eastern West Virginia, USA. Our objectives were to quantify the overall rate of trout movement and relate movement behaviors to variation in streamflow, water temperature, and access to coldwater refugia. The study area experienced extremely high seasonal, yearly, and among‐stream variability in water temperature and flow. The relative mobility of brook trout within the upper Shavers Fork watershed varied significantly depending on whether individuals resided within the larger main stem or the smaller tributary. The movement rate of trout inhabiting the main stem during summer months (50 m/d) was an order of magnitude higher than that of tributary fish (2 m/d). Movement rates of main‐stem‐resident brook trout during summer were correlated with the maximum water temperature experienced by the fish and with the fish's initial distance from a known coldwater source. For main‐stem trout, use of microhabitats closer to cover was higher during extremely warm periods than during cooler periods; use of microhabitats closer to cover during warm periods was also greater for main‐stem trout than for tributary inhabitants. Main‐stem‐resident trout were never observed in water exceeding 19.5°C. Our study provides some of the first data on brook trout movements in a large Appalachian river system and underscores the importance of managing trout fisheries in a riverscape context. Brook trout conservation in this region will depend on restoration and protection of coldwater refugia in larger river main stems as well as removal of barriers to trout movement near tributary and main‐stem confluences.
Application of a Bioenergetics Model for Hatchery Production: Largemouth Bass Fed Commercial Feeds
Authors: Csargo Isak J , Brown Michael L , Chipps Steven R 
Published: June 2012

Abstract
Fish bioenergetics models based on natural prey items have been widely used to address research and management questions. However, few attempts have been made to evaluate and apply bioenergetics models to hatchery-reared fish receiving commercial feeds that contain substantially higher energy densities than natural prey. In this study, we evaluated a bioenergetics model for age-0 largemouth bass Micropterus salmoides reared on four commercial feeds. Largemouth bass (n ≈ 3,504) were reared for 70 d at 25°C in sixteen 833-L circular tanks connected in parallel to a recirculation system. Model performance was evaluated using error components (mean, slope, and random) derived from decomposition of the mean square error obtained from regression of observed on predicted values. Mean predicted consumption was only 8.9% lower than mean observed consumption and was similar to error rates observed for largemouth bass consuming natural prey. Model evaluation showed that the 97.5% joint confidence region included the intercept of 0 (−0.43 ± 3.65) and slope of 1 (1.08 ± 0.20), which indicates the model accurately predicted consumption. Moreover model error was similar among feeds (P = 0.98), and most error was probably attributable to sampling error (unconsumed feed), underestimated predator energy densities, or consumption-dependent error, which is common in bioenergetics models. This bioenergetics model could provide a valuable tool in hatchery production of largemouth bass. Furthermore, we believe that bioenergetics modeling could be useful in aquaculture production, particularly for species lacking historical hatchery constants or conventional growth models.
Effectiveness of Copper Sulfate and Potassium Permanganate on Channel Catfish Infected with Flavobacterium columnare
Authors: Farmer Bradley D , Beck Benjamin H , Straus David L 
Published: June 2012

Abstract
Copper sulfate (CuSO4) and potassium permanganate (KMnO4) were evaluated for their effectiveness to curtail mortality and decrease bacterial load in fish tissues and water in channel catfish Ictalurus punctatus infected with Flavobacterium columnare, the causative agent of columnaris. Fish (average size, 16.6 g and 13.7 cm) were obtained from a single culture tank undergoing an epizootic of columnaris and were stocked at a density of 30 fish per tank in each of 12 tanks. The study consisted of three treatments (n = 4 replicates per treatment): an untreated control group, a CuSO4-treated group, and a KMnO4-treated group. Treatment rates were 2.1 mg/L CuSO4 and 3.0 mg/L KMnO4 and were applied on three consecutive days at 24-h intervals. Caudal fin, gill, and liver samples were taken from one fish of each replication 24 h after the last treatment for analysis by quantitative real time PCR. A significant improvement in survival was noted among fish treated with CuSO4 (72.7%) compared with the control fish (38.5%) and the KMnO4-treated fish (52.2%). Survival of the KMnO4-treated fish was not significantly different from the control. Quantitative PCR indicated a significant reduction of bacteria in the water after the first and second treatments of both therapeutants; no difference was detected in fish tissues. Histological analysis of gill samples 24 h after the last treatment indicated that CuSO4-treated fish exhibited significantly less gill damage than untreated controls and there was no difference in KMnO4-treated fish. Copper sulfate therapy significantly improved survival, reduced the number of bacteria in the water, and reduced gill pathologies associated with F. columnare infection in channel catfish.
Greetings from the new Editor-in-Chief, Ronald Stambaugh
Authors: Stambaugh Ronald 
Published: June 2012

Due to a processing error, this article was omitted from the printed copy of volume 52, issue 4 of Nuclear Fusion. The missing editorial is as follows:
I am very pleased to join the outstanding leadership team for the journal Nuclear Fusion as Scientific Editor. The journal's high position in the field of fusion energy research derives in no small measure from the efforts of the IAEA team in Vienna, the production and marketing of IOP Publishing, the Board of Editors led by its chairman Mitsuru Kikuchi, the Associate Editor for Inertial Confinement Max Tabak and the outgoing Scientific Editor, Paul Thomas. During Paul's five year tenure submissions have grown by over 40%. The usage of the electronic journal has grown year by year with about 300 000 full text downloads of Nuclear Fusion articles in 2011, an impressive figure due in part to the launch of the full 50 year archive. High quality has been maintained while times for peer review and publishing have been reduced and the journal achieved some of the highest impact factors ever (as high as 4.27).
The journal has contributed greatly to building the international scientific basis for fusion. I was privileged to serve from 2003 to 2010 as chairman of the Coordinating Committee for the International Tokamak Physics Activity (ITPA) which published in Nuclear Fusion the first ITER Physics Basis (1999) and its later update (2007). The scientific basis that has been developed to date for fusion has led to the construction of major facilities to demonstrate the production of power-plant relevant levels of fusion reactions. We look forward to the journal continuing to play a key role in the international effort toward fusion energy as these exciting major facilities and the various approaches to fusion continue to be developed.
It is clear that Nuclear Fusion maintains its position in the field because of the perceived high quality of the submissions, the refereeing and the editorial processes, and the availability and utility of the online journal. The creation of the Nuclear Fusion Prize, led by the Board of Editors chairman Mitsuru Kikuchi, for the most outstanding paper published in the journal each year has furthered the submission and recognition of papers of the highest quality. The accomplishments of the journal's team over the last five years will be a tough act to follow but I look forward to working with this competent and dedicated group to continue the journal's high standards and ensure that Nuclear Fusion remains the journal of choice for authors and readers alike.
Integrating literature-constrained and data-driven inference of signalling networks
Authors: Eduati Federica , De Las Rivas Javier , Di Camillo Barbara , Toffolo Gianna , Saez-Rodriguez Julio 
Published: June 2012

Abstract
Motivation: Recent developments in experimental methods facilitate increasingly larger signal transduction datasets. Two main approaches can be taken to derive a mathematical model from these data: training a network (obtained, e.g., from literature) to the data, or inferring the network from the data alone. Purely data-driven methods scale up poorly and have limited interpretability, whereas literature-constrained methods cannot deal with incomplete networks.
Results: We present an efficient approach, implemented in the R package CNORfeeder, to integrate literature-constrained and data-driven methods to infer signalling networks from perturbation experiments. Our method extends a given network with links derived from the data via various inference methods, and uses information on physical interactions of proteins to guide and validate the integration of links. We apply CNORfeeder to a network of growth and inflammatory signalling. We obtain a model with superior data fit in the human liver cancer HepG2 and propose potential missing pathways.
Availability: CNORfeeder is in the process of being submitted to Bioconductor and in the meantime available at www.cellnopt.org.
Contact: saezrodriguez@ebi.ac.uk
Supplementary information: Supplementary data are available at Bioinformatics online.