bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparative study to evaluate the effect of two different abutment designs on soft tissue healing and stability of mucosal margins
Authors: Patil Ratnadeep , van Brakel Ralph , Iyer Kavita , Huddleston Slater James , de Putter Cornelis , Cune Marco 
Published: December 2011

AbstractAimTo evaluate the effect of two different abutment designs on soft tissue healing and the stability of the mucosal margin in vivo.Materials and methodsTwenty‐nine subjects received two, non‐adjacent endosseous implants in the esthetic zone. Subsequently, conventional (control) and curved abutments (experimental) were placed in combination with a temporary restoration (left–right randomization). Plaster models of the healed sites were made to assess the stability of the soft tissues at baseline and after 6 weeks. To measure deseating force, a dontrix gauge was used while removing the abutments after 6 weeks.ResultsAlthough visually, differences in the transmucosal area were observed, the differences in marginal recession and in deseating force between abutments from the experimental and the control group never reached a statistically significant level. In general, some gain in soft tissue height was seen in both groups. Angled abutments elicited recession at all buccal sites (P = 0.003–0.02).ConclusionAbutments with a circumferential groove do not lead to a different response of the mucosal margin compared with a regular abutment, and are no more resistant upon removal than regular abutments after 6 weeks of function.
A 6‐month histological analysis on maxillary sinus augmentation with and without use of collagen membranes over the osteotomy window: randomized clinical trial
Authors: Barone A. , Ricci M. , Grassi R. F. , Nannmark U. , Quaranta A. , Covani U. 
Published: December 2011

AbstractIntroductionOver the years, several modifications have been made to the sinus augmentation technique and to the materials used. However, there is still controversy about the need for using a barrier concurrently with a graft in sinus augmentation procedures. On this basis, the aim of this randomized clinical study was to investigate the effect of resorbable collagen membrane over the osteotomy window on maxillary sinus augmentation healing.Materials and methodsPatients who required maxillary sinus augmentation were evaluated and selected to enter the study. After maxillary sinus grafting, each patient was randomly assigned to control (membrane over the osteotomy window) or test (no membrane) group. After 6 months, one bone biopsy was harvested from the lateral window and sent to the histology laboratory. The Mann–Whitney nonparametric test was used for comparing the two groups. P‐value was set at 5%.ResultsEighteen patients entered the study and were randomly allocated in control (nine patients) or test group (nine patients). The histomorphometric measurements revealed that newly formed bone was 30.7% ± 15.5% of the total volume from the membrane group (control). The average percentage of connective tissue was 50.6% ± 18.7% and residual graft percentage was 18.4% ± 20.3%. On the other hand, data regarding the nonmembrane group (test) showed that the percentage of newly formed bone was 28.1% ± 19.4%. The mean percentage of connective tissues was 59.3% ± 15.4% and 12.6% ± 12.4% for the residual graft particles. No significant difference was detected in the histomorphometrical evaluation between the two groups.DiscussionOur results showed that, compared with sites not covered, the use of the membrane did not substantially increase the amount of vital bone over a period of 6 months. On the other hand, the use of membrane seems to reduce the proliferation of the connective tissue and the graft re‐absorption rate. It is plausible that blood supply of maxillary sinus can play a role in such a result. Further studies are needed to explore whether the use of membrane could really be advantageous for the sinus augmentation procedure and to evaluate what influence this method can have on the amount and quality of reconstructed bone.
Osteotome technique with injectable tissue‐engineered bone and simultaneous implant placement by cell therapy
Authors: Yamada Yoichi , Nakamura Sayaka , Ueda Minoru , Ito Kenji 
Published: December 2011

AbstractObjectivesThe purpose of this study was to evaluate the effects of tissue‐engineered bone (TEB) on osteotome technique with simultaneous implant placement that is applied to the severe bone resorption in the maxilla for earlier bone regeneration and minimal invasive operation.Material and methodsWe applied injectable TEB, which was composed of bone marrow‐derived mesenchymal stem cells (BMMSCs) as stem cell source and platelet‐rich plasma as an autologous scaffold and signal molecules, with osteotome technique for 23 cases. After dental implants were pre‐prepared with pilot drills and/or using the Summers Osteotome Kits, TEB was injected and followed by dental implants insertion. The outcomes were evaluated by radiographs. Statistical evaluation was performed by Friedman test and Wilcoxon signed‐rank test (P ≤ 0.05).ResultsThe grafted BMMSCs possessed a phenotype of mesenchymal stem cells. The lift‐up bone height by TEB using BMMSCs showed the increase of 6.1 ± 1.5 mm. Moreover, the mean regenerated bone height was 8.2 ± 1.6 mm and 8.0 ± 1.4 mm, and the average alveolar bone height was 15.6 ± 1.2 mm and 15.1 ± 1.4 mm, at 3 and 6 months, respectively. There were significant differences between pre‐operative values and post‐operative ones (at 3, 6 months). No perforations of the Schneider membrane were found and the inserted implants were successful after 1 year.ConclusionsThis novel application using osteotome technique with injectable TEB would stably predict the success of bone formation and dental implants, and provide minimally invasive cell therapy.
Perceived behavioural control and coping planning predict dental brushing behaviour among Iranian adolescents
Authors: Pakpour Amir H. , Sniehotta Falko F. 
Published: December 2011

AbstractBackground and AimThe aim of this prospective study was to test the role of the direct predictors in the theory of planned behaviour (TPB), perceived behavioural control (PBC) and intention as well as action planning and coping planning as predictors of changes in dental brushing behaviour among Iranian adolescents.Materials and MethodsOne thousand students were selected from 57 high schools in Qazvin, IR Iran. N = 983 participants completed an initial questionnaire at Time 1 and they were asked to complete a follow‐up questionnaire 1 month later (Time 2). At Time 1, PBC, behavioural intention, action planning and coping planning were assessed. Dental brushing behaviour was assessed both at Time 1 and 4 weeks later at Time 2. A hierarchical regression analysis was conducted to examine the predictive validity of PBC, intention, action planning and coping planning both, as main effects and as interactions on dental brushing behaviour.ResultsPast behaviour, gender and age explained 46.5% of the variance in dental brushing at Time 2 in step 1. Adding intention to brush, PBC, action planning and coping planning in the second and third step explained an additional 24.9% of the variance in dental brushing at Time 2. Adding the interactions terms for action planning × coping planning, action planning × PBC and coping planning × PBC further increased the predictive validity of the model by 6.00% over and above the main effects.ConclusionIntentions, PBC, action planning and coping planning are predictive variables for oral self‐care behaviour. This finding suggests that an intervention simultaneously targeting PBC, action planning and coping planning might be particularly promising to promote oral self‐care in adolescence.
Successful implementation of cooperative handling eliminates the need for restraint in a complex non‐human primate disease model
Authors: Graham Melanie L. , Rieke Eric F. , Mutch Lucas A. , Zolondek Elizabeth K. , Faig Aaron W. , DuFour Theresa A. , Munson James W. , Kittredge Jessica A. , Schuurman Henk‐Jan 
Published: December 2011

AbstractBackground  Streptozotocin‐induced diabetic non‐human primates are used to study efficacy and safety of innovative immunosuppression after islet transplantation. We implemented a training program for medical management of a chronic disease state.Methods  Cooperation with hand feeding and drinking, shifting, and limb presentation were trained utilizing predominately positive but also negative reinforcement in 52 animals compared with 28 macaques subjected to conventional physical and/or chemical restraint. The success and timing of behavior acquisition was evaluated in a representative subset of 14 animals.Results  Over 90% of animals were successful in behavior acquisition. Programmatically this resulted in complete elimination of chair restraint and negligible requirement for sedation. About half of the trained animals had no‐to‐moderate thymic involution, indicative of a substantial reduction in stress.Conclusion  Cooperative handling enhances animal well‐being. This contributes to validity of scientific results and eliminates model‐induced confounding that can obstruct interpretation of safety and efficacy data.
Dental follicle cells and cementoblasts induce apoptosis of ameloblast‐lineage and Hertwig’s epithelial root sheath/epithelial rests of Malassez cells through the Fas–Fas ligand pathway
Authors: Lee Ji‐Hyun , Lee Dong‐Seol , Nam Hyun , Lee Gene , Seo Byoung‐Moo , Cho Young‐Sik , Bae Hyun‐Sook , Park Joo‐Cheol 
Published: December 2011

Lee J‐H, Lee D‐S, Nam H, Lee G, Seo B‐M, Cho Y‐S, Bae H‐S, Park J‐C. Dental follicle cells and cementoblasts induce apoptosis of ameloblast‐lineage and Hertwig’s epithelial root sheath/epithelial rests of Malassez cells through the Fas–Fas ligand pathway.

Eur J Oral Sci 2012; 120: 29–37. © 2011 Eur J Oral SciHertwig’s epithelial root sheath (HERS), epithelial rests of Malassez (ERM) cells, and reduced ameloblasts undergo apoptosis during tooth development. This study examined the effects of dental follicle cells and cementoblasts on the apoptosis of ameloblast‐lineage and HERS/ERM cells derived from the enamel organ. We also elucidated the induction pathways and identified the apoptotic pathway involved in this process. Here, we showed terminal deoxynucleotidyl transferase‐mediated biotin–dUTP nick‐end labeling (TUNEL)‐positive HERS cells and reduced ameloblasts near dental follicle cells during tooth development. Co‐culturing ameloblast‐lineage cell line (ALC) ameloblasts and HERS/ERM cells with either dental follicle cells or OCCM‐30 cementoblasts markedly enhanced the apoptosis of ameloblasts and HERS/ERM cells compared with cells cultured alone. However, dental follicle cells and cementoblasts did not modulate the apoptotic responses of co‐cultured non‐odontogenic MCF10A or KB cells. When ameloblasts + HERS and cementoblasts + dental follicle cells were co‐cultured, the expression of Fas ligand (FasL) increased in cementoblasts + dental follicle cells, while the expression of Fas increased in ameloblasts + HERS. Interestingly, recombinant FasL induced ameloblast apoptosis while the cementoblast‐induced ameloblast apoptosis was suppressed by the Fas/FasL antagonist Kp7‐6. These results suggest that during tooth development, dental follicle cells and cementoblasts induce apoptosis of ameloblast‐lineage and HERS/ERM cells through the Fas–FasL pathway, but do not induce the apoptosis of non‐odontogenic epithelial cells.
Wnt/β‐catenin pathway regulates bone morphogenetic protein (BMP2)‐mediated differentiation of dental follicle cells
Authors: Silvério K. G. , Davidson K. C. , James R. G. , Adams A. M. , Foster B. L. , Nociti F. H. , Somerman M. J. , Moon R. T. 
Published: December 2011

Silvério KG, Davidson KC, James RG, Adams AM, Foster BL, Nociti FH Jr, Somerman MJ, Moon RT. Wnt/β‐catenin pathway regulates bone morphogenetic protein (BMP2)‐mediated differentiation of dental follicle cells. J Periodont Res 2012; 47: 309–319. © 2011 John Wiley & Sons A/SBackground and Objective:  Bone morphogenetic protein 2 (BMP2)‐induced osteogenic differentiation has been shown to occur through the canonical Wnt/βcatenin pathway, whereas factors promoting canonical Wnt signaling in cementoblasts inhibit cell differentiation and promote cell proliferation in vitro. The aim of this study was to investigate whether putative precursor cells of cementoblasts, dental follicle cells (murine SVF4 cells), when stimulated with BMP2, would exhibit changes in genes/proteins associated with the Wnt/β‐catenin pathway.Material and Methods:  SVF4 cells were stimulated with BMP2, and the following assays were carried out: (i) Wnt/β‐catenin pathway activation assessed by western blotting, β‐catenin/transcription factor (TCF) reporter assays and expression of the lymphoid enhancer‐binding factor‐1 (Lef1), transcription factor 7 (Tcf 7), Wnt inhibitor factor 1 (Wif1) and Axin2 (Axin2) genes; and (ii) cementoblast/osteoblast differentiation assessed by mineralization in vitro, and by the mRNA levels of runt‐related transcription factor 2 (Runx2), osterix (Osx), alkaline phosphatase (Alp), osteocalcin (Ocn) and bone sialoprotein (Bsp), determined by quantitative PCR after treatment with wingless‐type MMTV integration site family, member 3A (WNT3A) and knockdown of β‐catenin.Results:  WNT3A induced β‐catenin nuclear translocation and up‐regulated the transcriptional activity of a canonical Wnt‐responsive reporter, suggesting that the Wnt/β‐catenin pathway functions in SVF4 cells. Activation of Wnt signaling with WNT3A suppressed BMP2‐mediated induction of cementoblast/osteoblast maturation of SVF4 cells. However, β‐catenin knockdown showed that the BMP2‐induced expression of cementoblast/osteoblast differentiation markers requires endogenous β‐catenin. WNT3A down‐regulated transcripts for Runx2, Alp and Ocn in SVF4 cells compared with untreated cells. In contrast, BMP2 induction of Bsp transcripts occurred independently of Wnt/β‐catenin signaling.Conclusion:  These data suggest that stabilization of β‐catenin by WNT3A inhibits BMP2‐mediated induction of cementoblast/osteoblast differentiation in SVF4 cells, although BMP2 requires endogenous Wnt/β‐catenin signaling to promote cell maturation.
Is religiosity related to periodontal health among the adult Jewish population in Jerusalem?
Authors: Zini A. , Sgan‐Cohen H. D. , Marcenes W. 
Published: December 2011

Zini A, Sgan‐Cohen H D, Marcenes W. Is religiosity related to periodontal health among the adult Jewish population in Jerusalem? J Periodont Res 2012; 47: 418–425. © 2011 John Wiley & Sons A/SBackground and Objective:  Religiosity may be a relevant protective factor for periodontitis, as it is for other chronic systemic diseases. The objective of the present study was to assess the relationship between periodontitis and religiosity, and whether oral health‐related behaviours, spirituality and social support are included in the potential pathways that explain the association between religiosity and periodontitis.Material and Methods:  Cross‐sectional data were part of a retrospective study. The stratified random‐sampling technique was limited to the Jerusalem Jewish population. Conceptual hierarchical data analysis modelling was adopted, assuming that socio‐economic position was the most distal determinant, age and gender were confounders, and social support, spirituality and oral health behaviours were mediators in the relationship between religiosity and periodontitis.Results:  Kappa intra‐examiner values (0.89) were satisfactory. Response rate was 88.0%. The final sample included 123 men and 125 women. The mean age was 38.6 years (SD 3.25 years), with 33.9% declared to be ‘orthodox’, 33.1%‘religious’ and 33.1%‘secular’. Higher levels of religiosity (p = 0.01), support of internal life through spirituality (p = 0.03), higher family social support (p = 0.02) and low levels of plaque (p = 0.05) were related to lower levels of periodontitis. Religiosity led to higher family social support and support of internal life through spirituality, which was related to plaque level and periodontitis.Conclusion:  Religiosity had a protective effect against periodontitis through extrinsic and intrinsic pathways. This should be considered as part of aetiology and prognosis, in potential prevention and care of periodontitis.
Using Clinical Pathways to Aid in the Diagnosis of Necrotizing Soft Tissue Infections Synthesis of Evidence
Authors: Schuster Lynn , Nuñez Diane E. 
Published: December 2011

ABSTRACTBackground: Necrotizing soft tissue infections (NSTIs) are rare, rapidly spreading infections that occur in the soft tissue compartments. The mortality rate is high and has been found to decrease if patients are treated early and aggressively with surgical debridement and broad‐spectrum antibiotics. Unfortunately these infections present similarly to other types of skin and soft tissue infections (SSTIs) making diagnosis difficult.Aims: This paper reviews the evidence surrounding the early diagnosis of NSTIs. This was used to develop a clinical practice guideline (CPG) for implementation in the emergency department (ED) setting to assist the provider in distinguishing NSTIs from SSTIs to potentially decrease the time from presentation to diagnosis.Methods: A review of the literature was performed. Studies were identified and critiqued by two reviewers independently for clinical relevance, study design, and statistical analysis.Results: Signs and symptoms, or “hard signs,” associated with NSTIs include: pain out of proportion to the exam, rapidly spreading infection, presence of bullae, skin ecchymosis or sloughing, gas in the tissues, skin anesthesia, edema extending beyond the erythema, and symptoms of sepsis. Unfortunately only 43% of the patients with an NSTI will present with these signs. Studies have found an association between laboratory values and NSTIs with the most commonly associated findings being leukocytosis, azotemia, and hyponatremia. Using these complimentary clinical and laboratory values, the Laboratory Risk Indicator for NECrotizing fasciitis (LRINEC) score is an emerging tool that providers can use to determine the risk of an NSTI. A clinical pathway was developed and implemented in the ED for all patients presenting with an SSTI to assist providers in confirming or negating the presence of an NSTI.Implication for Practice: Educating ED providers about the signs, symptoms, and laboratory findings associated with NSTIs will lead to earlier diagnosis and treatment and decreased morbidity and mortality.
Cloning and sequencing of Indian Water buffalo (Bubalus bubalis) interleukin‐3 cDNA
Authors: Thennarasu S. , Harishankar M. , Dhinakar Raj G. 
Published: December 2011

SummaryFull‐length cDNA (435 bp) of the interleukin‐3(IL‐3) gene of the Indian water buffalo was amplified by reverse transcriptase‐polymerase chain reaction and sequenced. This sequence had 96% nucleotide identity and 92% amino acid identity with bovine IL‐3. There are 10 amino acid substitutions in buffalo compared with that of bovine. The amino acid sequence of buffalo IL‐3 also showed very high identity with that of other ruminants, indicating functional cross‐reactivity. Structural homology modelling of buffalo IL‐3 protein with human IL‐3 showed the presence of five helical structures.
PROPIONIBACTERIUM FREUDENREICHII STRAINS AS ANTIBACTERIAL AGENTS AT NEUTRAL PH AND THEIR PRODUCTION ON FOOD‐GRADE MEDIA FERMENTED BY SOME LACTOBACILLI
Authors: DANILOVA IRINA V. , LEE HAO , TOUROVA TATYANA P. , RYZHKOVA EUGENIA P. , NETRUSOV ALEXANDER I. 
Published: December 2011

ABSTRACTPropionibacteria are food‐grade microorganisms that can suppress undesirable microbes in food. Profitability of their application depends on their effectiveness against food‐spoiling bacteria and on the accessibility of the protective product. In this study, the ability of Propionibacterium freudenreichii Pr4 to retard the growth of Bacillus subtilis, Bacillus cereus, Pseudomonas putida and Escherichia coli at neutral pH was proven. The efficiency of P. freudenreichii culture broth against all these bacteria, except E. coli, was similar to that of the chemical propionate, taken in the same amounts. Consequent cultivation with certain lactic acid bacteria was found to be a convenient way to achieve active growth of P. freudenreichii on food‐grade media. The pregrowth of Lactobacillus acidophilus 146 in skim milk and milk whey stimulated considerably the growth of P. freudenreichii Pr2 and the production of propionate. The final amount of P. freudenreichii Pr2 and L. acidophilus 146 viable cells in this case was 1.5 and 2 times more than when the bacteria grew separately. Other three Lactobacillus species tested did not provide stimulating effects. Sequential fermentation with Lactobacillus delbrueckii B‐3887 allowed to provide the growth of P. freudenreichii Pr4 in wheat flour medium and to get preparation that contains enough propionate for antirope bread protection.PRACTICAL APPLICATIONSNow we suggest a solid basis for the use of investigated strains of Propionibacterium freudenreichii in food bioprotection; first of all (directly due to our results) in bread manufacturing practice. They can be used as growth inhibitors of Bacillus subtilis, Escherichia coli and some Pseudomonas species not only in acidic but also in pH‐neutral conditions. The two‐stage cultivation of specially selected pairs of lactobacilli and propionibacteria is an approach for obtaining the propionibacteria‐containing preparations in economic and convenient way. The preparations can be applied for antibacterial protection of some other foods: dairy products, surfaces of cheeses, butter, sausages, etc.