bluuebunny/crossref_metadata_2025_split huggingface dataset:
Collagen type IV‐specific tripeptides for selective adhesion of endothelial and smooth muscle cells
Authors: Kanie Kei , Narita Yuji , Zhao Yingzi , Kuwabara Fumiaki , Satake Makoto , Honda Susumu , Kaneko Hiroaki , Yoshioka Tomohiko , Okochi Mina , Honda Hiroyuki , Kato Ryuji 
Published: February 2012

AbstractControlling the balance of endothelial cells (ECs) and smooth muscle cells (SMCs) in blood vessels is critically important to minimize the risk associated with vascular implants. Extracellular matrix (ECM) plays a key role in controlling the cellular balance, suggesting a promising source of cell‐selective peptides. To obtain EC‐ or SMC‐selective peptides, we start by highlighting sequence differences found among ECM molecules as enriched targets for cell‐selective peptides. We explored the EC‐ or SMC‐selective performance of tripeptides that are specifically enriched only in collagen type IV, but not in types I, II, III, and V. Collagen type IV was chosen since it is the major ECM in the basement membrane of blood vessels, which separates ECs and SMCs. Among 114 collagen type IV‐derived tripeptides pre‐screened from in silico analysis, 22 peptides (19%) were found to promote cell‐selective adhesion, as determined by peptide array. One of the best performing EC‐selective peptides (Cys‐Ala‐Gly (CAG)) was mixed into an electrospun fine‐fiber, a vascular graft material, for practical application. Compared to unmodified fiber, the CAG containing fiber surface was found to enhance adhesion of ECs (+190%) while limiting SMCs (−20%). These results are not only consistent with the hypothesis of ECM as a source of cell selective peptides, but also suggest a new genre of EC‐ or SMC‐selective peptides for tissue engineering applications. Collectively, these findings favorably support the screening approach used to discover new peptides for these purposes. Biotechnol. Bioeng. 2012; 109:1808–1816. © 2012 Wiley Periodicals, Inc.
Polyphenolic disaccharides endow proteins with unusual resistance to aggregation
Authors: Ladiwala Ali Reza A. , Perchiacca Joseph M. , Fishman Zachary S. , Bhattacharya Moumita , Hickey Anne Marie , Domigan Bonnie G. , Dordick Jonathan S. , Tessier Peter M. 
Published: February 2012

AbstractProtein aggregation is a common problem during the purification and formulation of therapeutic proteins. Here we report that polyphenolic disaccharides are unusually effective at preventing protein aggregation. We find that two polyphenolic glycosides—naringin and rutin—endow diverse proteins with the ability to unfold without aggregating when heated, as well as the ability to refold without aggregating when cooled at low glycoside concentrations (<5 mM). This extreme solubilizing activity is a synergistic combination of the glycone and aglycone moieties, as combinations of polyphenols and sugars fail to suppress aggregation. Moreover, the activity of polyphenolic disaccharides is remarkably specific since their monosaccharide counterparts (as well as other common excipients such as arginine, trehalose, and cyclodextrin) fail to prevent aggregation at similar concentrations (<25 mM). We expect that polyphenolic disaccharides will be valuable additives for enhancing the solubility of proteins in applications plagued by protein aggregation. Biotechnol. Bioeng. 2012; 109:1869–1874. © 2012 Wiley Periodicals, Inc.
A new electrochemical assay method for gene expression using hela cells with a secreted alkaline phosphatase (SEAP) reporter system
Authors: Şen Mustafa , Ino Kosuke , Shiku Hitoshi , Matsue Tomokazu 
Published: February 2012

AbstractA new electrochemical assay for the detection of secreted alkaline phosphatase (SEAP) from transfectant HeLa cells is proposed using a microarray device and scanning electrochemical microscopy (SECM). The assay consists of two steps: the first is the incubation of a transfected cell in a microarray culture device covered with a substrate modified with anti‐SEAP under physiological conditions without any additives. The array device consists of a 4 × 4 array of microwells having a size of 100 µm × 100 µm (diameter × depth). The second step is SECM measurement of secreted SEAP at the antibody‐immobilized substrate. This assay ensures accuracy and intactness because the undesired influence of endogeneous ALP is eliminated and the transfected cells are incubated in a culture device under suitable conditions. We successfully detected the expression of SEAP from intact cells at the single‐cell level using this assay. The system is useful as a cell‐based gene‐expression assay. Biotechnol. Bioeng. 2012; 109:2163–2167. © 2012 Wiley Periodicals, Inc.
Versatile Conversion of N‐Heterocyclic Silylene to Silyl Metal Compounds by Insertion of Divalent Silicon into Metal–Carbon and Metal–Hydrogen Bonds
Authors: Xiong Yun , Yao Shenglai , Driess Matthias 
Published: February 2012

AbstractThe facile one‐pot reaction of the stable N‐heterocyclic silylene LSi: 1 (L(ArN)C(CH2) CHC(Me)(NAr), Ar=2,6‐iPr2C6H3) with Me2Zn, Me3Al, H3Al‐NMe3, and MeLi has been investigated. The silicon(II) atom in 1 is capable of insertion into the corresponding MC and AlH bonds under very mild reaction conditions. Thus, Me2Zn furnishes the bis(silyl) zinc complex LSi(Me)ZnSi(Me)L 2 as the sole product, irrespective of the molar ratio of the starting materials applied. Moreover, the reactions of 1 with Me3Al, H3Al‐NMe3, and MeLi lead directly to the 1,1‐addition products LSi(Me)(Al(thf)Me2) 3, LSi(H)(AlH2(NMe3)) 4, and LSi(Me)Li(thf)3 5, respectively. All new compounds 2–5 were fully characterized by multinuclear NMR spectroscopy, mass spectrometry, elemental analyses, and single‐crystal X‐ray diffraction analyses.
Structure–Activity Relationship of Palladium Phosphanesulfonates: Toward Highly Active Palladium‐Based Polymerization Catalysts
Authors: Piche Laurence , Daigle Jean‐Christophe , Rehse Gregor , Claverie Jerome P. 
Published: February 2012

AbstractPalladium phosphanesulfonate [R2P(C6H4‐o‐SO3)PdMeL] catalysts permit the copolymerization of an exceptional large number of functional olefins with ethylene. However, these catalysts usually have reduced activity. We here have conducted a systematic study on the influence of the phosphane substituent, R, on activity and molecular weight. Phosphanes with strong σ‐donating character are shown to lead to the most active catalysts. Thus, the catalyst based on phosphane bis‐tert‐butyl‐phosphanyl‐benzenesulfonic acid (R=tBu) exhibits unprecedented high activity, rapidly polymerizing ethylene at room temperature to yield a linear polymer of high molecular weight (Mw=116 000 g mol−1). The influence of the R group on the catalyst ability to incorporate methyl acrylate is also investigated.
Porphyrin–Phthalocyanine/Pyridylfullerene Supramolecular Assemblies
Authors: Pereira Ana M. V. M. , Hausmann Anita , Tomé João P. C. , Trukhina Olga , Urbani Maxence , Neves Maria G. P. M. S. , Cavaleiro José A. S. , Guldi Dirk M. , Torres Tomás 
Published: February 2012

AbstractThe synthesis and photophysical properties of several porphyrin (P)–phthalocyanine (Pc) conjugates (P–Pc; 1–3) are described, in which the phthalocyanines are directly linked to the β‐pyrrolic position of a meso‐tetraphenylporphyrin. Photoinduced energy‐ and electron‐transfer processes were studied through the preparation of H2P–ZnPc, ZnP–ZnPc, and PdP–ZnPc conjugates, and their assembly through metal coordination with two different pyridylfulleropyrrolidines (4 and 5). The resulting electron‐donor–acceptor hybrids, which were formed by axial coordination of compounds 4 and 5 with the corresponding phthalocyanines, mimicked the fundamental processes of photosynthesis; that is, light harvesting, the transduction of excited‐state energy, and unidirectional electron transfer. In particular, photophysical studies confirmed that intramolecular energy‐transfer resulted from the S2 excited state as well as from the S1 excited state of the porphyrins to the energetically lower‐lying phthalocyanines, followed by an intramolecular charge‐transfer to yield P–Pc.+⋅C60.−. This unique sequence of processes opens the way for solar‐energy‐conversion processes.
Postoperative radiotherapy for elderly patients with stage III lung cancer
Authors: Wisnivesky Juan P. , Halm Ethan A. , Bonomi Marcelo , Smith Cardinale , Mhango Grace , Bagiella Emilia 
Published: February 2012

AbstractBACKGROUND:The potential role of postoperative radiation therapy (PORT) for patients who have completely resected, stage III nonsmall cell lung cancer (NSCLC) with N2 disease remains controversial. By using population‐based data, the authors of this report compared the survival of a concurrent cohort of elderly patients who had N2 disease treated with and without PORT.METHODS:By using the Surveillance, Epidemiology, and End Results (SEER) registry linked to Medicare records, 1307 patients were identified who had stage III NSCLC with N2 lymph node involvement diagnosed between 1992 and 2005. Propensity scoring methods and instrumental variable analysis were used to compare the survival of patients who did and did not receive PORT after controlling for selection bias.RESULTS:Overall, 710 patients (54%) received PORT. Propensity score analysis indicated that PORT was not associated with improved survival in patients with N2 disease (hazard ratio [HR], 1.11; 95% confidence interval [CI], 0.97‐1.27). Analyses that were limited to patients who did or did not receive chemotherapy, who received intermediate‐complexity or high‐complexity radiotherapy planning, or adjusted for time trends produced similar results. The instrumental variable estimator for the absolute improvement in 1‐year and 3‐year survival with PORT was −0.04 (95% CI, −0.15 to 0.08) and −0.08 (95% CI, −0.24 to 0.15), respectively.CONCLUSIONS:The current data suggested that PORT is not associated with improved survival for elderly patients with N2 disease. These findings have important clinical implications, because SEER data indicate that a large percentage of elderly patients currently receive PORT despite the lack of definitive evidence about its effectiveness. The potential effectiveness of PORT should be evaluated further in randomized controlled trials. Cancer 2012. © 2012 American Cancer Society.
Fitness model for tiered structure in the interbank market
Authors: Li Shouwei , He Jianmin 
Published: February 2012

AbstractTiered structure is observed in a range of countries' banking systems. In that case, relatively few first‐tier banks are not only interconnected, but are connected with second‐tier banks, whereas second‐tier banks are almost exclusively connected with first‐tier banks. This study uses the theory of complex networks to quantitatively characterize the formation of tiered structure in banking systems. The interbank market network model constructed in this article reproduces tiered structure and various statistical properties, namely, a small‐world property and a disassortative mixing property as well as a reciprocal property. This network modeling of the interbank market could be an efficient way to understand the bank behavior in the interbank market. © 2012 Wiley Periodicals, Inc. Complexity, 2012
A modeling approach for the non‐isothermal antisolvent crystallization of a solute with weak temperature dependent solubility
Authors: Widenski D. J. , Abbas A. , Romagnoli J. A. 
Published: February 2012

AbstractAntisolvent crystallization is a crystallization technique, which is normally operated isothermally. However, non‐isothermal operation is on occasions performed for solutes that have temperature dependent solubility. This paper shows that it is beneficial to operate antisolvent crystallizers non‐isothermally even for solutes whose solubility is weakly dependent on temperature. In this context, it demonstrates the joint control of particle mean size and size distribution coefficient of variation. A non‐isothermal crystallization model‐based framework is developed for the sodium chloride‐ethanol‐water system and validated for both isothermal and non‐isothermal operations. This framework was used to systematically determine both the optimal antisolvent feed rate and temperature profiles that minimize the coefficient of variation while producing a specified mean crystal size. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
The heteroepitaxial growth of KDP/ADP
Authors: Sun Yu , Yang Chunhui , Jiang Zhaohua , Wan Yuchun , Cheng Cheng , Meng Xiangbin , Hao Shuwei , Xu Chao 
Published: February 2012

AbstractCrystal growth rules of mixture crystals KADP (potassium dihydrogen phosphate (KDP) and ammonium dihydrogen phosphate (ADP)) have been analyzed based on the solubility product principle. The heteroepitaxial layers have been obtained by immersing KDP (ADP) substrate into the ADP (KDP) saturated solution at 313 K. The micromorphology indicates that small growing points on different planes show the self‐similar property compared to the bulk crystal's morphology. The process of epitaxial growth depends on not only form the lattice match but also form crystallizing kinetics which is the main influencing factor. Moreover, it can infer from the micromorphology on the surface of the mixed crystal that the dissolving of substrates will form mixed solution on epitaxial surface. What's more, corrosion phenomenon gets more and more evident with increasing times of epitaxial growth and it will be harder to form transparent epitaxial layers due to the increasing tension of epitaxial layers. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Investigating autism spectrum disorder and autistic traits in early onset eating disorder
Authors: Pooni Jyoti , Ninteman Aafke , Bryant‐Waugh Rachel , Nicholls Dasha , Mandy William 
Published: February 2012

AbstractObjective:To investigate whether young people (8–16 years) with an eating disorder have a higher prevalence of autism spectrum disorder (ASDs) and elevated autistic traits compared to typically developing (TD) peers.Method:Twenty‐two participants with early onset eating disorder (EOED) were assessed using standardized ASD measures and compared to IQ matched TD (n = 24) and ASD (n = 20) controls.Results:An ASD diagnosis was no more common in EOED than in TD controls. However, repetitive and stereotyped behavior was more often observed in the EOED group and, compared to TD controls, there was a trend (p = .07) toward greater autistic social impairment in EOED.Discussion:Whilst participants with EOED did not show increased ASD prevalence, they did have elevated autistic traits of clinical significance, particularly repetitive and stereotyped behavior. Further work is required to determine whether inflexibility and social difficulties in EOED have identical phenomenology and etiology to those seen in ASD. © 2012 by Wiley Periodicals, Inc. (Int J Eat Disord 2012)
The stigma of “mental” illness: End stage anorexia and treatment refusal
Authors: Campbell Amy T. , Aulisio Mark P. 
Published: February 2012

AbstractObjective:To answer the questions of whether psychiatric patients should ever be allowed to refuse life‐sustaining treatment in favor of comfort care for a condition that is caused by a psychiatric disorder, and if so, under what conditions.Method:Case discussion and normative ethical and legal analysis.Results:We argue that psychiatric patients should sometimes be allowed to refuse life—sustaining treatment in favor of comfort care for a condition that is caused by that psychiatric disorder and articulate the core considerations that should be taken into account when such a case arises.Discussion:We also suggest that unwillingness among many, especially mental health professionals, to consider seriously both of these questions risks perpetuating stigmatization of persons with psychiatric disorders, i.e., that the “mentally” ill should not be allowed to make significant decisions for themselves—a—a stigmatization that can result in persons with mental disorders both being prevented from exercising autonomous choice even when they are capable of it, and being denied good comfort care at the end of life——care which would be offered to patients with similarly life—threatening conditions that were not deemed to be the result of “mental” illness. © 2012 by Wiley Periodicals, Inc.
Development of the First Menthane‐Based Chiral Bis(π‐allylpalladium) Catalysis: Asymmetric Allylation of Imines
Authors: Fernandes Rodney A. , Chaudhari Dipali A. 
Published: February 2012

AbstractA new ethylidene menthane‐based chiral π‐allylpalladium complex catalyzes the asymmetric allylation of various imines with allyltributylstannane and 1 equiv. of water to give chiral homoallylamines in good yields and enantioselectivities. The reaction was carried out essentially under neutral conditions and displayed a good transfer of chiral information from the menthane skeleton through the formation of a bis(π‐allylpalladium) species. This is the first example of menthane‐based chiral bis(π‐allylpalladium) catalysis.
Concentration Dependence of Glycosylation Outcome: A Clue to Reproducibility and Understanding the Reasons Behind
Authors: Kononov Leonid O. , Malysheva Nelly N. , Orlova Anna V. , Zinin Alexander I. , Laptinskaya Tatiana V. , Kononova Elena G. , Kolotyrkina Natalya G. 
Published: February 2012

AbstractChanges in the concentration of reagents (0.009–0.2 M) have been shown to dramatically effect the yield and stereoselectivity of glycosylation with a sialic acid based glycosyl donor in a complex nonlinear manner that correlates with changes in the structures of the supramers of the reagents. The yield of disaccharide gradually increases with concentration and levels off at concentrations of glycosyl donor higher than 69 mM. The ratio of anomers is very high at some concentrations (α/β ≈ 20:1), moderate (α/β ≈ 8:1) or very low (α/β ≈ 4:1) at others. The formation of mixed supramers of glycosyl donor and glycosyl acceptor at concentrations exceeding 69 mM was detected by polarimetry and laser light scattering.
Copper‐Catalyzed Direct Thiolation of Pentafluorobenzene with Diaryl Disulfides or Aryl Thiols by C–H and C–F Bond Activation
Authors: Yu Chuanming , Zhang Cuiling , Shi Xiangjun 
Published: February 2012

AbstractA Cu‐catalyzed cross‐coupling reaction of diaryl disulfides or aryl thiols with pentafluorobenzene using CuBr as the catalyst, tBuOLi or tBuOK as the base in DMSO at 60 °C under an O2 atmosphere was investigated. The corresponding bisarylthiolation products were obtained in moderate to good yields by C–H bond and C–F bond activation. When 1,10‐phenanthroline·H2O and DDQ were added to the above system, monoarylthiolation products, with/without bisarylthiolation products, could be isolated in moderate yields. A plausible mechanism for these transformations was given. Thus, it represents a method for the synthesis of polyfluorinated diaryl sulfides and polyfluorinated triarylbisulfides throughC–H bond and C–F bond activation.
Modern pre‐injection in underground construction with rapid‐setting microcements and colloidal silica – applications in conventional and TBM‐tunnelling
Authors: Holter Karl‐Gunnar , Hognestad Hans‐Olav 
Published: February 2012

AbstractThe pre‐injection method of injection ahead of the excavation face in underground construction can offer significant advantages in many situations. This is particularly the case in difficult ground conditions with water ingress or in mechanically poor ground or soil, in which pre‐injection can help avoid problems and serious delays. Modern cost‐effective methods and material technology for pre‐injection in underground construction aim to achieve the desired result as fast as possible, hence reducing downtime during the excavation as much as possible. This paper reports a successfully resolved difficult case in which pre‐injection with rapid‐setting microcement was employed in combination with colloidal silica. The recent further improvement of the technical performance and versatility of rapid‐setting microcements through the use of controlled and precise addition of accelerators is also reported.Moderne Vorausinjektionen im Untertagebau mit schnell abbindenden Mikrozementen und kolloidaler Kieselsäure – Anwendungen im konventionellen und maschinellen TunnelbauDie Vorausinjektionsmethode oder Injektionen vor der Ortsbrust im Untertagebau bieten in vielen Situationen wesentliche Vorteile. Dies ist insbesondere der Fall bei schwierigen Baugrundverhältnissen wie dem Eindringen von Wasser oder Boden mit schlechten mechanischen Eigenschaften, in denen mittels Vorausinjektion Probleme und erhebliche Verzögerungen vermieden werden können. Moderne kostengünstige Methoden und Material‐Technologie für Vorausinjektionen im Untertagebau sollen das gewünschte Ergebnis möglichst schnell erzielen, um die Ausfallzeiten während des Vortriebs soweit wie möglich zu reduzieren. Dieser Beitrag berichtet über ein erfolgreich gelöstes Fallbeispiel, bei der die Vorausinjektion mit schnell abbindenden Mikrozementen in Kombination mit kolloidaler Kieselsäure angewandt wurde. Weiterhin wird über die aktuelle Verbesserung der technischen Leistungsfähigkeit und der Vielseitigkeit der schnell abbindenden Mikrozemente durch die kontrollierte, präzise Zugabe von Beschleunigern berichtet.
The AT ‐ Casing System / Das AT ‐ Hüllrohrsystem
Authors: Volkmann Günther M. , Reith Marco , Berner Thomas 
Published: February 2012

AbstractRecent developments show that special measures like pipe umbrellas are being used more often in continuous tunnelling, and already have to be planned during the design phase. The AT ‐ Casing System, already in successful use for some decades in sequential tunnelling and in geotechnical engineering, has recently seen increasing use in TBM tunnelling. According to the type of pipe used, the system can be used to support the surrounding ground, for grouting measures or for drainage. An example calculation is intended to demonstrate the potential of advance support over a fault zone and point out some possible optimisations of the load‐bearing capacity. Finally, the system is presented through the example of the New Kaiser Wilhelm Tunnel in Germany. On this project, the AT ‐ Pipe Umbrella System was combined with the AT ‐ GRP Injection System in order to gain the advantages of both.Die letzten Entwicklungen zeigen, dass Sondermaßnahmen wie Rohrschirme im kontinuierlichen Vortrieb häufiger eingesetzt werden und bereits im Planungsstadium berücksichtigt werden. Das AT ‐ Hüllrohrsystem wird bereits seit einigen Jahrzehnten im zyklischen Vortrieb und in der Geotechnik erfolgreich eingesetzt und in letzter Zeit auch vermehrt in Verbindung mit TVM. Je nach eingesetztem Rohrtyp kann es zum Stützen des Baugrunds, für Injektionsmaßnahmen oder für Dränagearbeiten eingesetzt werden. Ein Rechenbeispiel soll das Potenzial einer vorrauseilenden Stützung über eine Störungszone veranschaulichen und mögliche Optimierungen der Tragkraft aufzeigen. Zum Abschluss wird noch anhand des Projektbeispiels Neuer Kaiser Wilhelm Tunnel (Deutschland) eine exemplarische Anwendung dieses Systems erläutert. Bei diesem Projekt wurde das AT ‐ Rohrschirmsystem mit dem AT ‐ GFK‐Injektionssystem kombiniert, um die jeweiligen Vorteile zu nutzen.