bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interaction of Helicobacter pylori Infection and Nonsteroidal Anti‐Inflammatory Drugs in Gastric and Duodenal Ulcers
Authors: Venerito Marino , Malfertheiner Peter 
Published: July 2010

AbstractBackground:  Gastric (GU) and duodenal ulcers (DU) are in most instances either induced by Helicobacter pylori infection or by nonsteroidal anti‐inflammatory drugs (NSAIDs). Whether eradication of H. pylori is beneficial in NSAID users for preventing NSAID induced GU and DU has been the focus of different studies.Materials and Methods:  Mechanisms shared by both H. pylori and NSAIDs for the induction of GU and DU were reviewed and randomized controlled trials on H. pylori eradication for prevention and healing of GU and DU in patients requiring NSAID therapy were identified by a PubMed search.Results:  Key factors in the induction of GU and DU for both H. pylori and NSAIDs are a decrease in pH, imbalance between apoptosis and proliferation, reduction in mucosal blood flow, and recruitment of polymorphonucleates in distinct compartments. For primary ulcer prevention, H. pylori eradication before starting an NSAID therapy reduces the risk of NSAID induced GU and virtually abolishes the risk of DU. H. pylori eradication alone is not sufficient for secondary prevention of NSAID induced GU and DU. H. pylori infection appears to further increase the protective effects of proton‐pump inhibitors (PPI) to reduce the risk of ulcer relapse. H. pylori eradication does not influence the healing of both GU and DU if NSAID intake is discontinued.Conclusions:  Duodenal ulcer is more closely related to H. pylori infection than GU in NSAID users. H. pylori eradication is recommended for primary prevention of GU and DU in patients requiring NSAID therapy. PPI therapy is mandatory for secondary prevention of gastroduodenal ulcers, and appears to further reduce the risk of ulcer relapse in the presence of H. pylori.
Sequential Statistical Improvement of the Liquid Cultivation of Helicobacter pylori
Authors: Duque‐Jamaica Rocio , Arévalo‐Galvis Azucena , Poutou‐Piñales Raúl A. , Trespalacios Alba A. 
Published: July 2010

AbstractBackground:  Colonization of the gastric mucosa by Helicobacter pylori is one of the most important causes of acute and chronic gastric pathologies in humans. Achieving the growth of H. pylori in liquid media is of great importance in the development of clinical studies. In this study, we developed a sequential optimization strategy based on statistical models to improve the conditions of liquid culture of H. pylori.Materials and Methods:  Four statistical models were sequentially used. First, a Box‐Behnken design was used to select the best process conditions (shaking speed, inoculum concentration, and final volume of culture). Secondly, a general factorial design was used to evaluate the influence of adding gel blocks or gel beads (shape and composition). Then a D‐optimal reduce design was carried out to allow the selection of the most influential factors in increasing the cell concentration (culture media components). Finally, another Box‐Behnken design was used to optimize the concentration of the culture media components previously selected.Results:  After 12 hours of liquid culture a concentration of 25 × 108 cells per mL (9.4 log10 cells per mL) of H. pylori was obtained, compared with a predicted 32 × 108 (9.5 log10 cells per mL), which means between 1 and 5 log10 units higher than some previous reports.Conclusions:  The sequential statistical approach increased the planktonic H. pylori cell culture. The final culture media and conditions were: Brain Heart Infusion, blood agarose (1.5% w/v), lamb’s blood (3.18% v/v), DENT (0.11% v/v), and Vitox (0.52% v/v) at 60 rpm and 37 °C with filtered CO2 (5% v/v) bubbled directly into the culture media in a final volume of 76.22 mL.
Psychological outcome profiles in childhood‐onset temporal lobe epilepsy
Authors: Micallef Silvana , Spooner Claire G. , Simon Harvey A. , Wrennall Jacquie A. , Wilson Sarah J. 
Published: July 2010

SummaryPurpose:  To examine the effect of childhood‐onset temporal lobe epilepsy (TLE) on long‐term psychological function and to identify outcome profiles related to the natural course and treatment of TLE.Methods:  Psychological function was studied in a prospective, community‐based cohort of childhood‐onset TLE, approximately 13 years following seizure onset. Fifty‐three patients were assessed using a semi‐structured psychosocial interview, supplemented by self‐report questionnaires measuring quality‐of‐life, depression, self‐esteem, and anxiety.Results:  Common patterns were observed, giving rise to four distinct patient groups and psychological outcomes: (1) patients who experienced spontaneous remission of their seizures fared best; their psychological profile was characterized by heightened worry about the possibility of seizure recurrence; (2) patients who progressed to surgery and were seizure free reported adjustment difficulties associated with learning to become “well”; (3) patients who progressed to surgery and were not seizure free had the poorest psychological outcomes, with depression featuring prominently; and (4) patients with ongoing intractable epilepsy reported psychological and social features consistent with the effects of their chronic illness.Discussion:  Patients with childhood‐onset TLE face distinctive long‐term psychological challenges. The specific nature of these challenges can be understood in terms of the natural evolution and treatment of their epilepsy.
Persistence of lymphocyte function perturbations after granulocyte–colony‐stimulating factor mobilization and cytapheresis in normal peripheral blood stem cell donors
Authors: Marmier‐Savet Caroline , Larosa Fabrice , Legrand Faezeh , Witz Brigitte , Michallet Mauricette , Ranta Dana , Louvat Pascale , Puyraveau Marc , Pouthier Fabienne , Deconinck Eric , Tiberghien Pierre , Robinet Eric 
Published: July 2010

BACKGROUND: The short‐term effects of granulocyte–colony‐stimulating factor (G‐CSF) have been extensively studied, but recent reports of G‐CSF–induced genetic perturbations raised concerns regarding its long‐term safety. In this respect, duration of G‐CSF–induced perturbations has been less studied than short‐term effects and needs to be evaluated.STUDY DESIGN AND METHODS: G‐CSF mobilization–induced immunologic alterations were prospectively analyzed in a cohort of 24 healthy donors. Blood samples were taken before G‐CSF administration; at the time of administration; and at 1, 3, 6, and 12 months and analyzed for blood cell counts and in vitro cytokines (interleukin [IL]‐2, ‐8, and ‐10) and immunoglobulin production, quantified in the culture supernatant of peripheral blood mononuclear cells (PBMNCs) after, respectively, phytohemagglutinin and pokeweed mitogen stimulation.RESULTS: Platelet, granulocyte, monocyte, B, and dendritic blood cell counts as well as the IL‐2, ‐8, and ‐10 secretion by PBMNCs, perturbed at the time of G‐CSF mobilization, returned to baseline values at 1 month, with T‐cell and natural killer cell counts recovering at 3 months. In vitro immunoglobulin production was increased up to 6 months after mobilization.CONCLUSION: Although assessment of the potential long‐term risk of G‐CSF administration will require prolonged observation of larger cohorts, our data show that the duration of immunologic perturbations may be more persistent than previously anticipated, especially for B‐cell functional alterations. Most perturbations remain, however, transient with a return to baseline values within 1 year.
The extent of amotosalen photodegradation during photochemical treatment of platelet components correlates with the level of pathogen inactivation
Authors: Liu Weiqun , Cimino George D. , Corash Laurence , Lin Lily 
Published: July 2010

BACKGROUND: Amotosalen plus ultraviolet A (UVA) light inactivates a broad range of bacteria, viruses, protozoa, and leukocytes in platelet (PLT) and plasma components. Upon UVA illumination a small fraction of amotosalen reacts with the nucleic acid of contaminating pathogens and residual white blood cells and the remaining fraction undergoes photodegradation into defined photoproducts. The levels of amotosalen and photoproducts can be accurately quantified.STUDY DESIGN AND METHODS: This study evaluated the relationship between the extent of photodegradation of amotosalen and the level of pathogen inactivation in PLT components. PLT components containing of 3.78 × 1011 to 7.23 × 1011 PLTs in 300 to 450 mL of 35% to 50% plasma and 50% to 65% PLT additive solution and up to 5 × 106 red blood cells (RBCs)/mL were prepared. Each component was contaminated with 105 to 106 colony‐forming units/mL Klebsiella pneumoniae and treated with 115 to 200 µmol/L amotosalen and 0 to 3 J/cm2 UVA light. For each treatment condition, the level of K. pneumoniae inactivation (log‐reduction) was measured by microbiologic methods. The initial and postillumination amotosalen concentrations (µmol/L) were determined by high‐performance liquid chromatography.RESULTS: For a defined set of treatment conditions, the extent of amotosalen photodegradation was consistent and reproducible. The bacterial log‐reduction correlated linearly with the extent of amotosalen photodegradation with a regression correlation coefficient (r2) between 0.845 and 0.890 regardless of the treatment variables such as PLT content, component volume, plasma content, RBC content, initial amotosalen concentration, and UVA dose.CONCLUSION: This study demonstrated that the extent of amotosalen photodegradation can serve as an intrinsic actinometer which directly correlated with the level of pathogen inactivation.
Reduction of body iron stores to normal range levels in thalassaemia by using a deferiprone/deferoxamine combination and their maintenance thereafter by deferiprone monotherapy
Authors: Kolnagou Annita , Kleanthous Marios , Kontoghiorghes George J. 
Published: July 2010

AbstractBackground:  Iron overload and toxicity is the major cause of morbidity and mortality in thalassaemia patients. New chelating drug protocols are necessary to treat completely transfusional iron overload and eliminate associated toxicity. Appropriate deferiprone/deferoxamine combinations could achieve this goal.Methods:  A single‐centre, single‐armed, proof‐of‐concept study of the combination of deferiprone (75–100 mg/kg/d) and deferoxamine (40–60 mg/kg, at least 3 d per week) was carried out in eight patients with thalassaemia major (four men and four women) for 21–68 months. The patients were previously treated with deferoxamine and had variable serum ferritin [geometric (G) mean ± SD = 1446 ± 1035 μg/L] and magnetic resonance imaging relaxation times T2* cardiac (Gmean ± SD = 10.32 ± 6.72 ms) and liver (G mean ± SD = 3.77 ± 4.69 ms). The use of deferiprone (80–100 mg/kg/d) continued for 7–26 months in seven of the eight patients following the combination therapy. Organ function, blood and other biochemical parameters were monitored for toxicity.Results:  The deferiprone/deferoxamine combination caused an absolute value increase in cardiac (G mean ± SD = 29.6 ± 6.6 ms, P < 0.00076) and liver (G mean ± SD = 25.9 ± 8.07 ms, P < 0.00075) T2* and reduction in serum ferritin (G mean ± SD = 114.7 ± 139.8 μg/L, P < 0.0052) to within the normal body iron store range levels. In two cases, normalisation was achieved within a year. Deferiprone monotherapy was sufficient thereafter in maintaining normal range cardiac (G mean ± SD = 31.4 ± 5.25 ms, P < 0.79) and liver (G mean ± SD = 26.2 ± 12.4 ms, P < 0.58) T2* and normal serum ferritin (G mean ± SD = 150.7 ± 159.1, μg/L, P < 0.17) in five of the seven patients. No serious toxicity was observed.Conclusion:  Transfusional iron overload in patients with thalassaemia could be reduced to normal body iron range levels using effective deferiprone/deferoxamine combinations. These levels could be maintained using deferiprone monotherapy.
Traditions and reception: interpreting Vatican II's ‘Declaration on the Church's Relation to Non-Christian Religions’
Authors: D'Costa Gavin 
Published: July 2010

AbstractI examine the question of how tradition is received and passed on within the Church's Councils with specific attention to the problems of continuity and discontinuity. I use the example of Vatican II's ‘Declaration on the Church's Relation to Non-Christian Religions’ to explore the question of hermeneutics in both receiving and passing on the teachings of the Church. By looking at the historical development of this document I try to show that three important factors at work: first, the question of biblical interpretation; second, the question of determining which elements of tradition are authoritative and which not; and third, the influence of non-theological factors upon theological articulations. Through examining these factors, I argue for one particular approach to Council hermeneutics that is able to include and correct three other approaches. I argue that correct interpretations of Council documents do not represent the closure of tradition, but the opening up of tradition to future reception and re-formulation.(‘if anyone preaches a version of the Good News different from the one you have already heard, he is to be condemned.’) Galatians 1:9.
Theology in ‘contact with its own times’: Advertising and Evangelization
Authors: Sullivan Patricia A. 
Published: July 2010

AbstractThe Catholic Church's efforts at evangelization must include even its own members in an age of growing secularization wherein religious impulses can be subordinated to culture even unconsciously. Bernard Lonergan observed that religious values should transcend cultural and personal values. But prominent advertising confirms that in contemporary Western societies religious values are subsumed under others, even where spirituality may be held important. The goal before the Church, then, is a transposition of values possible in focus upon a common perception of theology and the culture reflected in advertising—the felt transcendence of the human person. The Second Vatican Council called for the Church to engage with the world; if the message served by the Church is to be heard above the din of the larger culture, it must be delivered in manners persuasive upon the sensibilities of contemporary people. The powerful cultural transmitter that is advertising may assist in this task, for study of the anthropologies of advertising and theology reveals certain surprising commonalities suggesting that methods, if not media, could be equally effective in both realms. Advertising's use of ethnography and narrative, in particular, may provide lessons for the Church in defining audiences and targeting the message to them.
MALE GAMETOPHYTE DEFECTIVE 2, Encoding a Sialyltransferase‐like Protein, is Required for Normal Pollen Germination and Pollen Tube Growth in Arabidopsis
Authors: Deng Yi , Wang Wei , Li Wen‐Qing , Xia Chuan , Liao Hong‐Ze , Zhang Xue‐Qin , Ye De 
Published: July 2010

Abstract Sialyltransferases (SiaTs) exist widely in vertebrates and play important roles in a variety of biological processes. In plants, several genes have also been identified to encode the proteins that share homology with the vertebrate SiaTs. However, very little is known about their functions in plants. Here we report the identification and characterization of a novel Arabidopsis gene, MALE GAMETOPHYTE DEFECTIVE 2 (MGP2) that encodes a sialyltransferase‐like protein. MGP2 was expressed in all tissues including pollen grains and pollen tubes. The MGP2 protein was targeted to Golgi apparatus. Knockout of MGP2 significantly inhibited the pollen germination and retarded pollen tube growth in vitro and in vivo, but did not affect female gametophytic functions. These results suggest that the sialyltransferase‐like protein MGP2 is important for normal pollen germination and pollen tube growth, giving a novel insight into the biological roles of the sialyltransferase‐like proteins in plants.
EFFECT OF THE ADDITION OF DEFATTED DATE SEEDS ON WHEAT DOUGH PERFORMANCE AND BREAD QUALITY
Authors: BOUAZIZ MOHAMED ALI , AMARA WAFA BEN , ATTIA HAMADI , BLECKER CHRISTOPHE , BESBES SOUHAIL 
Published: July 2010

ABSTRACT Defatted palm date seeds (date seed fiber concentrate: DSFC) of two varieties, Allig and Deglet Nour, were milled into two fractions: fine (particle size ≤ 280 µm) and coarse (280 µm < particle size ≤ 600 µm). DSFC was used to replace wheat flour in bread at 1 and 3% replacement levels. Dough and bread evaluation revealed that the addition of DSFC with fine size caused the decrease of bread volume, the change of crumb color and slightly increase bread crumb firmness. On the other hand, the addition of DSFC with a coarse size affected the mixing characteristics, decreased significantly the specific volume exceptionally at 3% and the bread crumb firmness (P < 0.05). An enrichment of bread with DSFC at 1 or 3% increased the dietary fiber contents in bread with lightly adverse effects on bread quality.PARACTICAL APPLICATIONTunisia is considered to be one of the dates‐producing countries. The mean annual yield of date fruits is about 125,000 tons. From this, around 12,500 tons of date seeds could be collected. This by‐product of date processing industries could be regarded as an excellent source of dietary fiber with interesting technological functionality and many beneficial effects on human health.Then, date seeds could present a value addition by extraction and use of date seed fiber concentrate in bread formulation.
RHEOLOGICAL CHARACTERIZATION OF PEACH PUREES
Authors: MASSA A. , GONZÁLEZ C. , MAESTRO A. , LABANDA J. , IBARZ A. 
Published: July 2010

ABSTRACT In this work, two commercial peach purees (21 and 11.9°Brix) industrially obtained have been studied at several temperatures involved in industrial processes. Viscoelastic functions were that of a weak gel, and decreased with temperature and increased with concentration. Rheograms were obtained in order to determine the yield stress, which is related to stability against settling during storage. Herschel–Bulkley equation was used to determine the consistency index and the flow index. Thixotropy was quantified by means of one empirical equation (Weltmann model) and two theoretical equations (Figoni–Shoemaker and Tiu–Boger models), which describe the first‐ and second‐order kinetic process of structure breakdown. Both peach purees show thixotropy under nonsteady conditions. The broken‐down structure increased with the applied shear rate and, practically, decreased as the temperature was increased for both peach purees. The breakdown kinetic constants decreased as the shear rate was increased for both theoretical equations following a power law dependency. PRACTICAL APPLICATIONSIt is necessary to know the thixotropic behavior of peach purees, since knowledge of the rheological properties is very important in order to improve the design, manipulation and calculation of all those unit operations during the process (i.e., agitation and filtration) and design equipment (i.e., pump systems and pipe sizing), mainly those based on heat and quantum transfer.
INFLUENCE OF PACKAGING CONDITIONS ON THE TEXTURAL AND SENSORY CHARACTERISTICS, MICROSTRUCTURE AND COLOR OF INDUSTRIALLY PRODUCED TURKISH WHITE CHEESE DURING RIPENING
Authors: AKALIN AYŞE SIBEL , KARAMAN AYŞE DEMET 
Published: July 2010

ABSTRACT A full‐fat Turkish white cheese was manufactured on an industrial scale and then packaged in a polystyrene container with brine containing 12% salt or a plastic pouch sealed under vacuum, before ripening for 90 days. With regard to textural characteristics, packaging in a plastic pouch resulted in cheeses with significantly higher hardness and cohesiveness (P < 0.05). Scanning electron micrographs showed a more compact and denser protein matrix for the cheese packed in the plastic pouch. Much yellowness was also obtained in cheeses with the plastic pouch (P < 0.05). The experimental cheeses had a similar sensory characteristics in the first 45 days (P > 0.05), but the taste and odor scores decreased in the cheeses packaged with the plastic pouch (P < 0.05) in the 60th and 90th days.PRACTICAL APPLICATIONSTurkish white cheese is generally ripened for 3 months in brine and marketed in a can or polystyrene package containing brine. Recently, cheese molds have been packaged in a plastic pouch without brine. The textural and sensory characteristics and color of the industrially produced Turkish white cheese packaged in a plastic pouch under vacuum or polystyrene container with brine were examined during storage. Higher hardness and cohesiveness was obtained by packaging in a plastic pouch; a more yellow color was also detected in the cheese with a plastic pouch. Sensory characteristics were not influenced by the packaging conditions during the first 45 days of storage, however, the taste and odor scores of the cheese with a plastic pouch were lower after 60 and 90 days.
MICROSTRUCTURAL, COMPOSITIONAL AND TEXTURAL PROPERTIES DURING RIPENING OF LIGHVAN CHEESE, A TRADITIONAL RAW SHEEP CHEESE
Authors: AMINIFAR MEHRNAZ , HAMEDI MANOUCHEHR , EMAM‐DJOMEH ZAHRA , MEHDINIA ALI 
Published: July 2010

ABSTRACT The physiochemical characteristics, microstructure and texture of Lighvan cheese were investigated over a 90‐day ripening period. The moisture content of the cheese decreased during storage and the salt‐in‐moisture ratio increased during this period. The most important biochemical change of the Lighvan cheese during aging was the extent of proteolysis. The water‐soluble nitrogen to total nitrogen ratio increased significantly during ripening. This microstructural study suggested that the size and number of pores increased significantly and the dense microstructure with large protein aggregates converted to a casein network with more homogenous aggregates. The degree of proteolysis caused the hardness of the ripened cheese to decrease during aging. PRACTICAL APPLICATIONSLimited work has been done on Lighvan cheese, a traditional Iranian cheese, which ripens in deep natural or man‐made caves for 3–4 months at an average temperature of 10–12C. Reducing ripening time is desirable for the producer. This study identified some of the physiochemical, microstructural and textural changes during cheese aging in brine. Precise recognition of cheese changes during aging provides essential information for ripening characteristics control and reducing ripening time.