bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unraveling Alveolar Fibroblast and Activated Myofibroblast Heterogeneity and Differentiation Trajectories During Lung Fibrosis Development and Resolution in Young and Old Mice
Authors: Lingampally Arun , Truchi Marin , Shi Xianrong , Zhou Yuqing , Vasquez‐Pacheco Esmeralda , Panagiotidis Georgios‐Dimitrios , Hadzic Stefan , Koepke Janine , Vazquez‐Armendariz Ana Ivonne , Herold Susanne , Samakovlis Christos , Cabrera‐Fuentes Hector A. , Chu Xuran , Seeger Werner , Zhang Jin‐San , El Agha Elie , Mari Bernard , Bellusci Saverio , Chen Chengshui 
Published: February 2025

ABSTRACTIdiopathic pulmonary fibrosis (IPF) is an age‐associated disease characterized by the irreversible accumulation of excessive extracellular matrix components by activated myofibroblasts (aMYFs). Following bleomycin administration in young mice, fibrosis formation associated with efficient resolution takes place limiting the clinical relevance of this model for IPF. In this study, we used aged mice in combination with bleomycin administration to trigger enhanced fibrosis formation and delayed resolution as a more relevant model for IPF. Alveolosphere assays were carried out to compare the alveolar resident mesenchymal niche activity for AT2 stem cells in young versus old mice. Lineage tracing of the Acta2+ aMYFs in old mice exposed to bleomycin followed by scRNAseq of the lineage‐traced cells isolated during fibrosis formation and resolution was performed to delineate the heterogeneity of aMYFs during fibrosis formation and their fate during resolution. Integration of previously published similar scRNAseq results using young mice was carried out. Our results show that alveolar resident mesenchymal cells from old mice display decreased supporting activity for AT2 stem cells. Our findings suggest that the cellular and molecular mechanisms underlying the aMYFs formation and differentiation towards the Lipofibroblast phenotype are mostly conserved between young and old mice. In addition to persistent fibrotic signaling in aMYF from old mice during resolution, we also identified differences linked to interleukin signaling in old versus young alveolar fibroblast populations before and during bleomycin injury. Importantly, our work confirms the relevance of a subcluster of aMYFs in old mice that is potentially relevant for future management of IPF.
Seeds of Sustainability: Unveiling the Population Dynamics of Carapa procera, Lophira lanceolata, and Pentadesma butyracea for Adaptive Oilseed Value Chains
Authors: Tiétiambou Fanta Reine Sheirita , Kangbéni Dimobe , Salako Kolawolé Valère , Lankoandé Benjamin , Ouédraogo Amadé 
Published: February 2025

ABSTRACTCarapa procera, Lophira lanceolata, and Pentadesma butyracea are important non‐timber forest products providing species with promising value chains of their oils. To assess whether traditional uses of these species are sustainable, this study analysed their population structure and regeneration patterns in non‐protected sites. Three sites where the fruits of these species are exploited for oilseed production and trade were selected for each species, and 135 plots with 665 subplots were established for adults and regenerations inventories, respectively. The population structure and regeneration patterns were assessed using dendrometric characteristics (tree diameter at breast height—dbh and height), population density, and metrics of stability. C. procera had the highest dendrometric values at Diossogo and Koloko for adult individuals. Its populations were generally stable across all sites. For L. lanceolata, the highest dendrometric values were observed at Natindougou. However, populations at both Natindougou and Nafanasso showed signs of disturbance and instability. For P. butyracea, the highest values for mean diameter and Lorey's height of adult individuals were recorded at Sokouraba. Population densities were highest at Nafanasso, but populations were disturbed at Sokoroni and Sokouraba, with stability observed across all sites. Given the current condition of the stands of each species, implementing management actions tailored to site‐specific conditions is essential to ensure the long‐term sustainability and productivity of these valuable species in their natural habitats.
Clinical effectiveness, feasibility, acceptability, and usability of mobile health applications for epilepsy: A systematic review
Authors: Gotlieb Evelyn , Marzoughi Shahab , Kwon Churl‐Su , Harmon Michael , Kimura Maren , Truesdale Ashley , Sweetnam Chloe , Soudant Céline , Downes Margaret H. , Busis Neil A. , Kummer Benjamin R. , Jetté Nathalie 
Published: February 2025

AbstractMobile applications are widely used in epilepsy, although their impact on clinical effectiveness (CE) and their feasibility, acceptability, and usability (FAU) remain unclear. We conducted a systematic review investigating CE and FAU of epilepsy mobile applications using MEDLINE and Embase from database inception to June 21, 2024. We followed Preferred Reporting Items for Systematic Reviews and Meta‐Analyses reporting standards. The protocol was registered on PROSPERO (CRD42019134848). In duplicate, we determined study quality using the Newcastle–Ottawa Quality Assessment Scale (NOQAS) and the Joanna Briggs Critical Appraisal Checklist (to determine eligibility for inclusion), risk of bias using the Cochrane Risk of Bias tool, and usability study quality using the 15‐point Silva scale. We identified 8953 studies, of which 20 were included. Twelve (60.0%) addressed CE, nine (45.0%) acceptability, five (25.0%) usability, and eight (40.0%) feasibility. Five (25.0%) evaluated CE and FAU. Studies comprised prospective cohort (n = 9, 45.0%), pilot (n = 3, 15.0%), randomized controlled trial (n = 7, 35.0%), and pre/post (n = 1, 5.0%) designs. Most apps were used for self‐management or to enhance education or communication between patients and providers. Cohort studies demonstrated fair quality (median NOQAS score = 5, interquartile range [IQR] = 5.0–5.8), whereas of seven randomized controlled trials, four (57.1%) had some concern for bias. Usability studies demonstrated high quality (median Silva score = 10, IQR = 10–11). Apps were predominantly intended for patient use (n = 9, 75.0%). Symptom reporting and medication management were the most common app targets in both CE and FAU studies (n = 8, 66.7%; n = 9, 69.2%), although FAU studies more frequently used monitoring or tracking (n = 10, 76.9%) and reminder setting (n = 10, 76.9%) than CE apps (n = 7, 58.3%). Investigations of application use most commonly studied CE and patient‐facing apps. Additional high‐quality evidence is necessary to evaluate the CE and FAU of app use in epilepsy to work toward the standardization of FAU metrics and development of implementation guidelines.
Habitat and Feeding Behaviour Influence Microbiome Composition in Rhabdocoela (‘Turbellaria’)
Authors: Bijnens Karolien , Monnens Marlies , Thijs Sofie , Artois Tom , Smeets Karen 
Published: February 2025

ABSTRACT1. Microbiome studies in Platyhelminthes have predominantly focused on a limited number of taxa, overlooking the vast diversity of turbellarian hosts. Here, we aimed to expand our understanding of microbial associations in a selection of free‐living representatives of Rhabdocoela, a group of turbellarian flatworms that is very species rich and ecologically diverse. 2. Using 16S rRNA sequencing, we characterised the microbiomes of three species of Rhabdocoela, representing the two most speciose lineages within this taxon: Dalytyphloplanida and Kalyptorhynchia. The specimens were captured in the wild and obtained from marine or freshwater habitats. 3. The microbiomes of Gyratrix hermaphroditus (Kalyptorhynchia) and Mesostoma ehrenbergii (Dalytyphloplanida) were both dominated by Proteobacteria, while Phaenocora evelinae (Dalytyphloplanida) was predominantly associated with Cyanobacteria, more specifically Oxyphytobacteria (chloroplasts). 4. Based on the observed genera, our analysis revealed distinct microbial patterns, possibly associated with the habitat and lifestyle of the studied species. We could not exclude the presence of a phylosymbiotic signal as a limited core microbiome was present for each rhabdocoel species, although no set of bacteria common to all three rhabdocoel species was found. 5. This explorative study contributes to the expanding knowledge of invertebrate microbiomes, providing new insights into the microbial associations of a selection of turbellarians. The descriptive results presented here open up several promising avenues for future research, including the search for functional roles of turbellarian bacterial symbionts and exploring potential correlations between microbiome compositions, turbellarian phylogeny and environmental variables.
A Rare Case of Optic Disc Swelling Associated With Herpes Simplex Virus Infection
Authors: Yamaoka Minako , Kataoka Hiroshi , Sugie Kazuma 
Published: February 2025

ABSTRACTOcular HSV infection is usually caused by herpes simplex virus (HSV) which establishes latency in the trigeminal ganglion. Ocular involvement presents as a manifestation of primary infection or the recurrence of latent disease and affects all ocular tissues. Meningitis or encephalitis typically manifests as headaches and meningeal symptoms and is frequently associated with papilledema, a condition caused by elevated intracranial pressure. Here, we describe a rare case of optic disc swelling as a manifestation of HSV infection. Although the patient did not experience meningeal signs, headaches, or apparent intracranial hypertension, he presented with optic disc swelling without any ocular or retinal disorders. The intrathecal antibody titer index for HSV increases with pleocytosis. Antiviral treatment significantly reduced optic disc swelling, suggesting an association between HSV infection and swelling. Optic disc swelling can be the only presentation of an HSV infection, and antiviral medication may be an effective treatment.
Profiling the Activity of the Potent and Highly Selective CDK2 Inhibitor BLU-222 Reveals Determinants of Response in CCNE1-Aberrant Ovarian and Endometrial Tumors
Authors: House Nealia C. , Brown Victoria E. , Chen Maxine , Yuan Liang , Moore Sydney L. , Guo Jian , Choi Yoon Jong , Muthuswamy Lakshmi , Ribich Scott , Ramsden Philip , Faia Kerrie L. 
Published: February 2025

Abstract
BLU-222 is an investigational, potent, highly selective, orally bioavailable CDK2 inhibitor in clinical development. BLU-222 demonstrated robust antitumor activity in select CCNE1-high ovarian and endometrial cancer models. We used a combination of CRISPR whole genome screens coupled with targeted genetic and pharmacologic approaches in ovarian and endometrial cell lines to identify biological determinants to predict BLU-222 monotherapy activity. Rb and p16 expression were biomarkers that enriched for CDK2-dependency/BLU-222 sensitivity in CCNE1 overexpressed, non-amplified cells. Further, intact Rb and low p16 expression predicted a BLU-222 and CDK4/6 inhibitor combination response. BLU-222 demonstrated robust activity in combination with carboplatin or paclitaxel in CCNE1-aberrant models, rendering chemotherapy-resistant tumors strongly sensitive to the combination. These findings demonstrate that response to CDK2 inhibition by BLU-222 can be further predicted using a combinatorial biomarker signature that could refine patient selection criteria in CCNE1-high patients and support clinical development.
Associations between Anti-Gay Prejudice, Traditional Masculine Self-Concept, and Colorectal Cancer Screening-Related Outcomes among Black and White Men in the US
Authors: Chen Tianen , Wicke Rebekah , King Andy J. , Margolin Drew , Chunara Rumi , Niederdeppe Jeff 
Published: February 2025

Abstract
Background: Colorectal cancer (CRC) screening can reduce CRC risk, yet many men are not up to date with screening guidelines. While previous qualitative studies have suggested links among anti-gay prejudice, traditional masculine self-concept, racial identity, and CRC screening among men, scholars have yet to fully explore these associations using quantitative data. This study used a nationally representative sample of Black and White men in the US to test these associations and examine the sociodemographic correlates. Methods: Using the NORC/AmeriSpeak probability-based panel, we recruited a sample of Black and White men in the US aged 45 to 74 who had never been diagnosed with CRC (N = 909). Participants completed an online questionnaire measuring anti-gay prejudice, traditional masculine self-concept, sociodemographic variables, and screening-related outcomes (awareness of screening test options, screening intention, and adherence to screening recommendations). Results: Black participants reported higher levels of anti-gay prejudice and traditional masculine self-concept than White participants. Anti-gay prejudice was associated with lower awareness and lower screening intention. Black participants reported higher intention to follow screening recommendations but not higher odds of actual adherence than White participants. Conclusions: Men with anti-gay prejudice are less likely to be aware of CRC screening test options and less likely to intend to engage in CRC screening. Results have implications for the design and development of future interventions aimed at increasing CRC screening rates. Impact: Future studies could develop targeted interventions and observe subsequent changes or conduct longitudinal studies to further explore the role of anti-gay prejudice in CRC screening.
Superresolution based on coherent thermal radiation with selective information
Authors: Huang Duan-Hsin , Chang Chih-Wei 
Published: February 2025

Abstract
We reexamine superresolution methods that may have been overlooked by previous optical microscopy techniques. For a one-dimensional (1D) system, we show that maximizing the information capacity of an imaging system is not a necessary condition for surpassing the Abbe diffraction limit. Specifically, the spatial resolution of two coherent emitters can go beyond the Abbe diffraction limit if an appropriate information zone, but not the full information zone, is selected for far-field imaging. Based on this principle, we show that λ/2.6 superresolution can be easily achieved for two coherent thermal radiative sources with a sufficiently large phase difference. Similar effects can be found for a 1D array of thermal radiative sources coupled by surface phonon polaritons. Introducing a dielectric microsphere into the system can further enhance the phase difference among the radiative sources, achieving superresolution better than λ/4. The concept and method presented here can be implemented to enhance the spatial resolution of thermal imaging.
Influence of ambient light on the accuracy of different face scanning methods: an in-vitro study
Authors: Keil Paul Ulrich , Beuer Florian , Unkovskiy Alexey , Atay Ece , Jennes Marie-Elise 
Published: February 2025

Abstract

Background
Face scanners provide a viable method for capturing a patient’s face geometry. To optimize their accuracy, influencing factors, like the ambient light, need to be examined.

Methods
A human head model with eight pins attached to its surface was used to investigate the accuracy of four face scanning methods (Face Hunter, iPad, Medit i700, single camera photogrammetry) under three illumination levels (500 lx, 5000 lx, 20 000 lx). An industrial CT scan was used as reference. Two alignment-areas – full face (AL-FF) and spheres (AL-KG) and two investigation areas – center face (UB-CF) and full face (UB-FF), were used during the examination. The root-mean-square-error (RMSE) was employed as a measure. Separated by trueness and precision, a one-way ANOVA was performed with post hoc Games-Howell tests for each scanning method.

Results
All scanners showed significant differences between the illumination levels. For most test groups, the Face Hunter acquired its lowest RMSE values under 500 lx. The same can be said for the Medit i700, even though for trueness, differences to 5000 lx were random. Single camera photogrammetry performed better at higher illumination levels, but only random differences between 5000 lx and 20 000 lx were seen. For the iPad, different results for optimal illumination were found regarding trueness and precision, as well as the investigation areas. All accuracy results were labelled as highly reliable, except for the iPad´s trueness results.

Conclusion
Scanner-dependent influence of ambient light was shown in this in-vitro study. Face Hunter and Medit i700 performed better under a darker illumination of 500 lx, whereas single camera photogrammetry needed brighter lighting. For the iPad no tested lighting situation showed clear advantages.
Ecofriendly and smart spectrophotometric approaches for synchronized analysis of two antipsychotic drugs, fluoxetine and olanzapine: application to combined tablet dosages with assessment of method greenness
Authors: Derayea Sayed M , Madian Huda , Samir Ebtihal , Badr-eldin Khaled M. 
Published: February 2025

Abstract
The present study was dedicated for solving the challenge of the overlapped spectra of olanzapine (OLZ) and fluoxetine (FLX) for their concurrent, accurate and precise determination without prior physical separation. The developed methods had to venture away from those that depend on chromatography, because of their negative effects on the environment due to using hazardous organic solvents as well as their high cost. The suggested methods were based on spectrophotometry combined with simple mathematical treatments of the spectra. These approaches are simple, rapid, inexpensive and reliable options for the effective assay of FLX and OLZ in pharmaceutical dosage forms. Ratio subtraction method was devoted for estimation of both drugs, while dual wavelength method and absorptivity factor method could be utilized in the estimation of FLX and OLZ, respectively. All the suggested methods were efficiently applied for the determination of the studied drugs in laboratory prepared mixtures and their pharmaceutical formulations. The environmental safety of the involved procedures was assessed by applying the Eco Score scale and complex modified GAPI methods. The methods were proved to be highly safe since no large volume of solvents were used, and no derivatizing reagent or drastic experimental conditions were involved. In addition, the procedures consume little energy and produce a small amount of waste. In conclusion, for FLX determination, the use of dual wavelength method is preferable because it needs very low mathematical treatment than the ratio subtraction method. However, for OLZ, the ratio subtraction method has higher sensitivity than absorptivity factor method although the latter is simpler.
Sensitive and selective silver nanoparticle-based fluorescence sensor for corticosteroid determination in pharmaceutical formulations
Authors: Elawadi Ghidaa G. , Elsebaei Fawzi , Fathy Mona E. , Metwally Mohammed E.-S. 
Published: February 2025

Abstract
Glucocorticoids play a crucial role in metabolic processes and have potent anti-inflammatory and immunosuppressive properties. Hence, developing a facile, sensitive, selective, and green approach to determine corticosteroids is essential. In this study, silver nanoparticles (Ag-NPs) were synthesized via the chemical reduction of silver nitrate using sodium borohydride in distilled water without using non-environmentally friendly organic stabilizers. The synthesized Ag-NPs exhibited high stability, as evidenced by a zeta potential measuring − 36.8 mV. Also, the average particle size was determined to be 8 ± 2 nm. These Ag-NPs were then employed as a nano fluorescence probe to establish a fluorometric assay for determining prednisolone sodium phosphate (PDN) and dexamethasone sodium phosphate (DXZ). Reduction in fluorescence intensity of Ag-NPs observed at 484 nm following excitation at 242 nm exhibited quantitative quenching upon the incremental addition of the investigated drugs, with limits of detection of 0.178 µg/mL and 0.145 µg/mL for PDN and DXZ, respectively. The quenching mechanisms were examined and explained using the Stern-Volmer and Inner Filter Effect methods. The method’s selectivity was also assessed by testing other corticosteroids. The proposed method is suitable for drug testing in pharmaceutical products and quality control labs. It follows ICH guidelines and has been confirmed to be safe and eco-friendly.
Twenty-four years of prescription patterns in bipolar disorder inpatients with vs without lithium: a pharmacoepidemiological analysis of 8,707 cases in German-speaking countries
Authors: Greil Waldemar , de Bardeci Mateo , Nievergelt Nadja , Erfurth Andreas , Hasler Gregor , Bridler Rene , Toto Sermin , Grohmann Renate , Seifert Johanna , Schoretsanitis Georgios 
Published: February 2025

Abstract

Background
Global pharmacoepidemiological evidence suggests dynamically changing prescription patterns in patients with bipolar disorders. We assessed trends in the use of pharmacological agents used in the management of bipolar disorders in inpatients.

Methods
We examined drug use data provided by the Drug Safety in Psychiatry Programme AMSP (German: “Arzneimittelsicherheit in der Psychiatrie”), including psychiatric hospitals in Germany, Austria and Switzerland. We included data from adult inpatients with bipolar disorders (ICD-10: F31) treated between 1994 and 2017. We compared prescription patterns between patients receiving therapeutic regimens with vs. without lithium. Patients with manic and depressive episodes were also analyzed separately.

Results
We identified a total of 8,707 patients (58% females, mean age 50.8 ± 14.8 years). Our analysis revealed a decrease of lithium use (up to 2004) and a consistent increase of prescription rates for second-generation antipsychotics (SGA) among which quetiapine (n = 2,677) and olanzapine (n = 1,536) were the most common. Among psychotropic drugs, quetiapine was most frequently combined with lithium (n = 716, 25.6%). Lithium-treated patients received a higher number of drugs compared to patients not receiving lithium (mean number of drugs in patients with vs. without lithium 4.99, n = 2,796 vs. 4.75, n = 5,911, p = 0.002). Thyroid therapeutics were given more often, valproate and quetiapine less often in the lithium group. Antidepressants were consistently prescribed to more than 60% of patients with bipolar depressive episodes.

Conclusions
Our findings suggest that SGAs are gradually becoming the mainstay treatment option in bipolar disorder, continuously replacing lithium. The use of antidepressants remains concerningly high. We call for action to improve adherence to evidence-based guidelines.