bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discovery of highly potent and novel LSD1 inhibitors for the treatment of acute myeloid leukemia: structure-based virtual screening, molecular dynamics simulation, and biological evaluation
Authors: Hong Ye , Wang Yuting , Hao Ziyi , Zhang Xingxia , Si Yejun , Lin Guoqiang , Zhang Shurong , Niu Miao-Miao , Yang Xiaotian , Zhang Yanming 
Published: February 2025

Acute myeloid leukemia (AML) is a highly aggressive hematological malignancy with a significant unmet clinical need for new therapeutic agents. Lysine-specific demethylase 1 (LSD1), a key regulator of leukemia stem cell self-renewal, has emerged as a promising epigenetic target for AML treatment. Herein, we employed an innovative multi-step integrated screening protocol, encompassing pharmacophore modeling, docking screening, molecular dynamics simulation, and biological evaluation, to identify novel LSD1 inhibitors. This comprehensive approach led to the discovery of six potent LSD1 inhibitors (we named these inhibitors LTMs 1–6), with LTM-1 exhibiting the most pronounced inhibitory effects on LSD1 (IC50 = 2.11 ± 0.14 nM) and the highest selectivity for LSD1 over LSD2 (&gt;2370-fold). Notably, LTM-1 demonstrated outstanding antitumor activity both in vitro and in vivo. In vitro, LTM-1 showed potent anti-proliferative effects against LSD1-addicted MV-4-11 leukemia cells (IC50 = 0.16 ± 0.01 μM). In vivo, LTM-1 treatment significantly reduced tumor growth in MV-4-11 xenografted mice. Moreover, LTM-1 did not induce significant changes in liver and kidney function indices, suggesting a favorable safety profile. These results indicate that LTM-1 is a highly promising preclinical candidate for AML treatment, offering a new strategy for the development of more effective and selective LSD1 inhibitors.
In vitro and in vivo characterization of oridonin analogs as anti-inflammatory agents that regulate the NF-κB and NLRP3 inflammasome axis
Authors: Ou Huiping , Wu Zhanpan , Ning Jinhua , Huang Qiufeng , Wang Wancun , Yang Guochun , Zhou Yingxun , Hou Anguo , Li Peng , Chen Lingyun , Jin Wen Bin 
Published: February 2025

IntroductionA series of oridonin hybrids were synthesized and evaluated for anti-inflammatory potential, focusing on their ability to inhibit NO production in RAW264.7 cells and their therapeutic prospects for NLRP3-driven disorders.MethodsAnti-inflammatory activity was assessed by measuring NO inhibition in LPS-stimulated RAW264.7 cells. The most active compound, 4c, was further analyzed using ELISA and WB to evaluate its effects on inflammatory proteins (p-NF-κB, p-IκB, NLRP3, IL-6, IL-1β, COX-2, iNOS). In vivo efficacy was tested in a murine acute lung injury model, with RT‒qPCR and WB used to assess inflammatory markers in lung tissues. Molecular docking predicted 4c’s binding mode with NLRP3, while RNA-seq and RT‒qPCR identified differentially expressed genes.ResultsCompound 4c significantly inhibited NO production and suppressed key inflammatory proteins in vitro. In vivo, it alleviated acute lung injury, reduced IL-6 and TNF-α mRNA levels, and inhibited NLRP3, p-NF-κB, and IL-6 protein expression. Docking suggested covalent binding to NLRP3. RNA-seq revealed 4c upregulated Trdc, Stfa2, and Gsta2 while downregulating Spib, Csf2, and Nr4a1.DiscussionCompound 4c demonstrates potent anti-inflammatory effects via NLRP3 pathway inhibition and modulation of inflammatory genes. These findings highlight oridonin hybrids, particularly 4c, as promising candidates for NLRP3-driven inflammatory disorders, warranting further investigation.
Does the occurence of homostyly necessarily accompany the breakdown of heteromorphic incompatibility system?
Authors: Zhao Jing , Kuliku Laiziti , Zhang Aiqin , Jiao Fangfang , Ren Dengfu 
Published: February 2025

IntroductionHeterostyly is a genetic polymorphism that facilitates precise pollen transfer through reciprocal herkogamy. The loss or variation of reciprocal herkogamy is usually accompanied by the breakdown of heteromorphic incompatibility system. Homostyly, which is characterized by self-compatibility and same stigma-anther height is a common floral morph in the variation and evolution of heterostyly. Limonium aureum is a distylous species distributed in the desert of northwest China, in which a floral morph with the same stigma-anther height (H-morph) widely distributed in the natural populations, resembling classical homostyly. The aim of this study was to clarify whether the occurrence of H-morph is also accompanied by the breakdown of heteromorphic incompatibility system, and the relationship between the H-morph and long-styled-/shortstyled-morph (L-/S-morph).MethodsThe floral morphs composition and frequency, heterostylous syndrome, pollinators and visiting efficiency were investigated in five natural populations of L. aureum based on field observation, artificial control pollination experiment and so on.Results and conclusionAll populations were composed of L-, S- and H-morphs, except for ATS population with only H-morph, and there were significant differences in flower size parameter, fruit set, and degree of pollination limitation, while no differences among morphs within population. However, each population demonstrated dimorphic pollen-stigma morphology and a strict heteromorphic incompatibility system, especially ATS population, in which they were compatible between morphs with heteromorphic pollen-stigma morphology, regardless of the reciprocal herkogamy, and vice versa. It is speculated that the H-morph in different populations may be at different stages of heterostylous evolution. The ATS population may be a dimorphic population without reciprocal herkogamy which is in the stage before distyly formation, while the other 4 populations may be dimorphic populations with significant variation in reciprocal herkogamy which is in the stage after distyly formation. The H-morph may be caused by stigma-anther separation shortening of L- and S-morph in other 4 populations. These phenomenons that the variation of floral morph is independent of physiological incompatibility breakdown, as well as the coexistence of populations from different origins and evolutionary stages within the same species have been reported for the first time in the Plumbaginaceae.
A study of the effect of question feedback types on learning engagement in panoramic videos
Authors: Huang Guan , Zhang Haohua , Zeng Jingsheng , Chen Wen 
Published: February 2025

IntroductionThe immersive and interactive nature of panoramic video empowers learners with experiences that are infinitely close to the real environment and increases the use of imagination in learners’ knowledge acquisition. Studies have shown that embedding question feedback in traditional educational videos can effectively improve learning. However, little research has been conducted on embedding question feedback in panoramic videos to explore what types of question feedback effectively improve the dimensions of learners’ learning engagement and yield better learning experiences and learning effects.MethodsThis study embedded questions with feedback within panoramic videos by categorizing feedback into two types: simple feedback and elaborated feedback. Using eye tracking, brainwave meters, and subjective questionnaires as measurement tools, this study investigated which type of question feedback embedded in panoramic videos improved various dimensions of learner engagement and academic performance. Participants (n = 91) were randomly assigned to the experimental group (simple feedback, elaborated feedback) or the control group (no feedback).ResultsThe results of the study showed that (1) the experimental group significantly improved in cognitive engagement, behavioral engagement, and emotional engagement compared to the control group. When the precision of feedback information was greater, the learner’s behavioral engagement was greater; however, the precision of feedback information did not significantly affect cognitive and emotional engagement. (2) When the feedback information was more detailed, the learners’ academic performance was better.DiscussionThe findings of this study can support strategic recommendations for the design and application of panoramic videos.
Mental health amidst multiple crises: trends and sociodemographic risk factors in Austria’s general population
Authors: Humer Elke , Pieh Christoph , Probst Thomas , Dinhof Carina , Schaffler Yvonne , Zeldovich Marina 
Published: February 2025

BackgroundIn recent years, mental health in Austria has faced substantial challenges due to a series of both global and regional crises.ObjectiveThis study aimed to assess changes in mental health indicators within the Austrian population over time from April 2022 to October 2024 and to identify sociodemographic correlates of poor mental health.MethodsTwo cross-sectional online surveys on representative samples of the Austrian general population were conducted at two timepoints: April 2022 (n = 1,032) and October 2024 (n = 2,025). Mental health indicators, including depression, anxiety, insomnia, alcohol misuse, and stress, were measured using validated scales. Sociodemographic data were collected to examine associations with mental health indicators. Chi-squared tests and t-tests were conducted to compare mental health indicators between 2022 and 2024, and multivariable logistic regression models were applied to examine associations with sociodemographic data.ResultsClinically relevant symptoms of depression and high stress decreased from 2022 to 2024 (depression from 28.3% to 21.6%, stress from 56.3% to 51.0%; p &lt; 0.01), while other symptoms remained stable. Sociodemographic analysis revealed that female gender, younger age, lower income, unemployment, migration background and lack of partnership were associated with higher odds for several mental health issues.ConclusionsFindings suggest small improvements in mental health from 2022 to 2024 and identified sociodemographic risk factors linked to mental health vulnerabilities.
From activity to obesity: understanding gender and type of school divide among Saudi adolescents
Authors: Said Mohamed Ahmed , Alibrahim Mohammed Shaab 
Published: February 2025

BackgroundObesity has emerged as a significant predictor of the nationwide burden of non-communicable diseases in Saudi Arabia.ObjectivesThis study explores patterns in body mass index (BMI), physical activity (PA), and sedentary behavior (SB) among Saudi adolescents, focusing on variations by gender and school type (public vs. private). It identifies key factors affecting BMI.MethodsA total of 2,815 students (53.64% male, aged 15.21 ± 1.55 years) participated. Body weight and composition were determined by bioelectrical impedance, while PA levels and SB scores were assessed through validated questionnaires. Two models were created, one with overall SB and the other with specific SBs.ResultsAmong participants, 28.4% were overweight/obese, with 17.2% classified as obese. Obesity prevalence was higher in boys (26.9%) compared to girls (6.0%). Overweight prevalence was slightly lower in private schools (9.9%) than in public schools (11.8%), while obesity rates were similar (17.3% vs. 17.2%). A significant association between BMI and school type was identified solely in girls (Model 1: p = 0.004, ES = 0.109; Model 2: p = 0.012, ES = 0.096). Age was positively associated with BMI (Model 1: p &lt; 0.004, ES = 0.025; Model 2: p &lt; 0.001, ES = 0.019), as were SB scores (p &lt; 0.001, ES = 0.17). Conversely, PA levels exhibited a negative correlation with BMI (Model 1: p &lt; 0.001, effect size = −0.104; Model 2: p &lt; 0.001, effect size = −0.106). Polynomial analysis revealed a cubic relationship between BMI and PA across all groups, though with low effect sizes.ConclusionGender, age, PA, and SB explained a small portion of BMI variance. Future research should investigate mechanisms underlying these non-linear trends and explore additional confounding variables.
A smart classifier of orthoses, prostheses and special materials (OPMEs) in invoices
Authors: Gomes Wesckley , Colaço Júnior Methanias , Alves Luca Pareja Credidio Freire , Fontes Raphael , Silva Rodrigo , Nunes Bruno , Silva Caldeira , Valentim Ricardo 
Published: February 2025

ContextThe OPME (Órteses, Próteses e Materias Especiais or Orthoses, Prosthetics and Special Materials) Brazilian sector presents a wide variety of products and technologies, involving both multinational and local companies in healthcare. Despite technological advances, many services and information systems, especially in the public sphere, still use unstructured natural language descriptions of products, services or events, making their classification and analysis difficult. However, for efficient audits, it is necessary to classify and totalize invoices issued for product purchases automatically. In this way, the standardization lacking regarding nomenclature in the OPME marketing not only makes it difficult to compare products, whether for price standardization or standardization of use but also opens up space for possible acts of corruption.ObjectiveTo mitigate the problem of ineffective standardization and coding, develop and assess the effectiveness and efficiency of an OPME classifier, in the context of electronic invoice descriptions, from the point of view of auditors, healthcare professionals, and data scientists.MethodControlled Experiment, to evaluate scientifically mapped Artificial Intelligence (AI) algorithms and compare accuracy measures, F1-Score, sensitivity, precision, average training time, and classification.ResultsWith an accuracy of 99%, the Linear Support Vector algorithm stood out among the others in terms of accuracy, while Naïve Bayes in terms of efficiency, had the fastest average training time.ConclusionThe results showed that it is possible to identify and classify OPMEs in invoices automatically. This allows for a more precise and effective analysis of signs such as anomalously high prices and quantities of OPMEs purchased per inhabitant, which are analyzed by the Audit of Brazil’s Unified Health System (AudSUS), Ministry of Health -Brazil, for identification of potential irregularities and contribution to transparency and efficiency in the management of health resources.
Changes in stride pattern of elite women's 400 metres hurdles from 2019 to 2022: an analysis by performance level
Authors: Casal-García Nerea , López-Del Amo José Luis 
Published: February 2025

IntroductionThe main objective of this study was to investigate the stride patterns of elite women's 400 m hurdles which have undergone significant evolution in recent years, resulting in a complete change in the stride patterns of the athletes.MethodsTwo hundred and fifty-five individual performances of all participants in the World Championships in Doha 2019 and Oregon 2022, the Tokyo 2020 Olympic Games and the 2022 European Championships were analysed based on video recordings taken during the competition. The entire sample was divided into 3 performance-level groups (higher performance, intermediate performance, and lower performance). Correlation analysis, analysis of variance (Kruskall-Wallis) and linear multiple regression were performed.Results and discussionA total of 18 stride patterns have been identified, of which the most used by the highest performing athletes (58%) was the n + 1 pattern (consisting of adding a step between hurdle's intervals) maintaining 15 steps up to the 8th hurdle and then switching to 16 steps between hurdles 8th and 10th. In contrast, the majority of LP athletes used an n + 1 + 1 strategy, transitioning from 15 to 16 steps between the 5th and 6th hurdles (59%) and further increasing to 17 steps between hurdles 7th and 10th. Moreover, high-performance athletes take fewer strides during the race, with significant differences observed across all race intervals, particularly in the second half of the race. The number of steps between hurdles 8 and 9 predicted 78% of the ΔR2 of the athlete's total number of steps during the race, highlighting the importance of stride pattern and its changes during the final phase of the race.
Case report: Pesticide-related methemoglobinemia: Tebufenozide and indoxacarb poisoning
Authors: Wang Jieru , Yu Guangcai , Jian Tianzi , Kan Baotian , Li Wei , Jian Xiangdong 
Published: February 2025

BackgroundMethemoglobinemia can be inherited or acquired. Acquired forms are more common due to drugs or poisonous substances that oxidize hemoglobin, and pesticide-related cases are notably rare.Case PresentationWe report a 69-year-old woman who ingested 30 mL of tebufenozide and indoxacarb and was asymptomatic for 3 h; however, the patient was admitted to the hospital after 8 h, unconscious, with tachypnea, cyanosis, and 61.9% methemoglobin. The patient was administered methylene blue, mechanically ventilated, and hemoperfused. Subsequently, the patient recovered and was discharged with no discomfort and with normal laboratory test results.ConclusionTebufenozide and indoxacarb may cause methemoglobinemia, leading to cyanosis, unconsciousness, and respiratory failure; therefore, they should be handled with care in clinical practice.
RANCANG BANGUN APLIKASI PENGELOLAAN PESANAN PADA UMKM BAKSO "RIZKI BAROKAH" DI ERA DIGITAL
Authors: Ibad Sholihul , Fatah Mochamad Nur , Fitriani Ayu Santi Nur , Saputra Yumiyanto Cahya 
Published: February 2025

Aplikasi berbasis desktop merupakan teknologi berbasis komputer yang berguna untuk memberikanÂ kemudahan dalam mengelola data sehingga mampu menghasilkan sebuah laporan dengan cara yang efisien. Warung Bakso Rizki barokahÂ adalah UMKM yang mempersajikan berbagai olahan bakso dan memiliki harga terjangkau. Karena harganya terjangkau menjadikannya mulai dikenalÂ masyarakat kalangan menengah ke bawah sehingga mengharuskannya menerima pemesanan yang cukupÂ banyak. Tentu, hal ini akan menjadi sebuah masalah ketika pengelolaan data pemesanan hingga pembayaranÂ tidak dikerjakan dengan baik. Apalagi selama ini pengelolaannya masih bersifat manual sehingga karyawanÂ harus bekerja lebih teliti dalam mencatat pemesanan hingga pembayaran pesanan, serta laporan yang rawan akan kekeliruan . Untuk itu, perlu dilakukan sebuah perancangan serta pembangunan sebuah aplikasi berbasisÂ desktop dalam mengelola data tersebut. Pada penelitian ini akan dirancang dan dibangun sebuah aplikasiÂ dengan cara study literatur - survei UMKM - analisis sistem - implementasi sistem .Ini akan jadiÂ aplikasi berbasis desktop untuk mengelola data pesanan , maka dengan aplikasi berbasis desktop yang dibangun dapat membantu dalam mengelola dataÂ pesanan dan pembayaran oleh pemilik dan karyawan Bakso Rizki barokah.DESIGN AND DEVELOPMENT OF AN ORDER MANAGEMENT APPLICATION FOR MSME BAKSO "RIZKI BAROKAH" IN THE DIGITAL ERADesktop-based applications are computer-based technology that is useful for providing convenience in managing data so that it can produce reports in an efficient way. Warung Bakso Rizki Barokah is an MSME that serves various processed meatballs and has affordable prices. Because the price is affordable, it is starting to become known to the lower middle class, which requires it to receive quite a lot of orders. Of course, this will be a problem if the management of order data and payment is not done properly. Moreover, so far the management is still manual, so employees have to work more carefully in recording orders and paying for orders, as well as reports that are prone to errors. For this reason, it is necessary to design and build a desktop-based application to manage this data. In this research, an application will be designed and built by means of literature study - MSME survey - system analysis - system implementation. This will be a desktop-based application for managing order data, so the desktop-based application that is built can help in managing order and payment data by owner and employee of Bakso Rizki Barokah.
APPLICATION OF INFORMATION TECHNOLOGY THROUGH DESKTOP-BASED APPLICATIONS TO HELP THE OPERATIONAL PRODUCTIVITY OF THE UMKM “KOPI PINGGIR KALI” (KPK)
Authors: Ibad Sholihul 
Published: February 2025

This research describes the creation of a desktop-based cash register application that aims to increase operational productivity at MSMEs "Kopii Pingir Kali" (KPK) located in Boto Village, Semanding District, Tuban Regency. This application is designed to solve various problems faced by MSMEs such as: Examples include manual transaction entry, which is time-consuming and error-prone, as well as difficulty tracking daily inventory and revenue. Using the waterfall software development methodology, this cashier application was developed with key features such as sales recording, inventory management, daily sales report, and food menu data management. The system was tested based on simulated transactions and user feedback and showed improved efficiency in transaction processing and report management. The introduction of this application aims to enable MSMEs "Kopi Pinggir Kali" (KPK) to improve the accuracy of transaction recording, speed up the sales process and provide more accurate and timely reports. The results of this study show that the use of information technology in MSME operations can have a positive impact on business productivity and efficiency, and provide MSMEs with practical solutions to overcome daily operational challenges.PENERAPAN TEKNOLOGI INFORMASI MELALUI APLIKASI BERBAIS DEKSTOP UNTUK MEMBANTU PRODUKTIFITAS OPERASIONAL UMKM “KOPI PINGGIR KALI” (KPK)Penelitian ini menjelaskan tentang pembuatan aplikasi mesin kasir berbasis desktop yang bertujuan untuk meningkatkan produktivitas operasional pada UMKM “Kopii Pingir Kali” (KPK) yang berlokasi di Desa Boto, Kecamatan Semanding, Kabupaten Tuban. Aplikasi ini dirancang untuk menyelesaikan berbagai permasalahan yang dihadapi UMKM seperti: Contohnya termasuk entri transaksi manual, yang memakan waktu dan rawan kesalahan, serta kesulitan melacak inventaris dan pendapatan harian. Dengan menggunakan metodologi pengembangan perangkat lunak air terjun, aplikasi kasir ini dikembangkan dengan fitur-fitur utama seperti pencatatan penjualan, pengelolaan inventaris, laporan penjualan harian, dan pengelolaan data menu makanan. Sistem diuji berdasarkan simulasi transaksi dan umpan balik pengguna meningkatkan akurasi pencatatan transaksi, mempercepat proses penjualan dan memberikan laporan yang lebih akurat dan tepat waktu. Hasil penelitian ini menunjukkan bahwa penggunaan teknologi informasi dalam operasional UMKM dapat memberikan dampak positif terhadap produktivitas dan efisiensi bisnis, serta memberikan solusi praktis kepada UMKM untuk mengatasi tantangan operasional sehari-hari.
Efektivitas Formulasi Sabun Pembersih Dari Minyak Hasil Samping Pengalengan
Authors: Zahra Widyasari , Nurjanah Nurjanah , Prasetiyo Himawan , Rudi Mad 
Published: February 2025

Pengolahan hasil samping menjadi solusi untuk permasalahan limbah. Penelitian ini bertujuan untuk mengetahui efektifitas pada formulasi sabun dari hasil samping pengalengan ikan. Hasil samping pengalengan sebelum digunakan sebagai bahan baku pembuatan sabun diolah terlebih dahulu melalui proses pre-treatment. Adsorben yang digunakan sebagai media filtrasi dalam mengkondisikan minyak ikan yaitu adsorben kombinasi antara arang aktif dan zeolite. Penelitian ini menggunakan analisis data secara kuantitatif dan dijelaskan secara deskriptif. Sabun pembersih padat minyak ikan diuji melalui proses uji hedonik dengan jumlah 25 orang dengan menggunakan sabun komersil sebagai kontrol. Adsorben yang dipilih berdasarkan penerimaan uji hedonik dan penilaian uji gores (streak plate method) karena keefektifannya dalam membunuh kuman selama 60 menit. Minyak hasil samping pengalengan ikan dapat digunakan sebagai bahan baku dalam pembuatan produk sabun pembersih.