bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pendampingan Sistem Pemasaran Berbasis Online pada Produk Batik Badan Usaha Milik Desa (BUMDes) Mulya Abadi di Dusun Tirta Mulya Kabupaten Bungo
Authors: Suryanti Krisna , Latifah Aisyah  , Putri Rizky Ramadhani  
Published: June 2024

Abstract
The Mulya Abadi Village-Owned Enterprise (BUMDes) which is engaged in batik marking business activities known as Batik Tirta Mulya is a business activity that has prospects from a social and economic perspective. However, so far BUMDes Mulya Abadi products are still marketed or sold only to local residents, even though by utilizing technological developments, the sales system can be carried out online as a promotional medium, with the hope that the marketing segment will not only be local, but can reach the national level. and even international. The aim of this community service is to provide knowledge and skills to BUMDes management staff through the use of e-commerce technology to expand product sales marketing. The results obtained from this service are in the form of an e-commerce website, which can reach wider marketing and it is hoped that product sales will increase. Apart from that, consumers can also save time, costs and energy and make it easier to make transactions or get to know Batik Tirta Mulya even though they don’t come to Dusun Tirta Mulya.
Keywords: Marketing, BUMDes, E-commerce, Batik.
Abstrak
Badan Usaha Milik Desa (BUMDes) Mulya Abadi yang bergerak dalam kegiatan usaha penandaan batik yang dikenal dengan nama Batik Tirta Mulya merupakan kegiatan usaha yang mempunyai prospek dari segi sosial maupun ekonomi. Namun, selama ini produk-produk BUMDes Mulya Abadi masih dipasarkan atau dijual hanya kepada warga sekitar, padahal dengan memanfaatkan perkembangan teknologi, sistem penjualan dapat dilakukan berbasis online sebagai media promosinya, yang diharapkan segmen pemasarannya tidak hanya bersifat lokal, namun dapat menjangkau hingga tingkat nasional dan bahkan internasional. Tujuan pengabdian masyarakat ini adalah untuk memberikan pengetahuan dan keterampilan kepada perangkat pengelola BUMDes melalui pemanfaatan teknologi e-commerce untuk memperluas pemasaran penjualan produk. Hasil yang diperoleh dari pengabdian ini berupa website e-commerce, yang dapat menjangkau pemasaran menjadi lebih luas dan diharapkan penjualan produk semakin meningkat. Selain itu, konsumen juga dapat menghemat waktu, biaya, dan tenaga serta memudahkan dalam bertransaksi atau mengenal Batik Tirta Mulya meskipun tidak datang ke Dusun Tirta Mulya.
Kata Kunci: Pemasaran, BUMDes, E-commerce, Batik.
Evaluasi Performa Basis Data Distribusi Menggunakan Teknologi Cloud Computing
Authors: Alfeno Sandro , Suhaepi Muhamad Iip , Aprian Dzikra 
Published: June 2024

Penelitian ini dilakukan untuk mengevaluasi penggunaan teknologi cloud computing dalam meningkatkan performa basis data distribusi dan membandingkannya dengan lingkungan non-cloud dalam suatu perusahaan atau organisasi yang menggunakan teknologi ini. Metode penelitian yang digunakan untuk penelitian ini adalah metode kepustakaan. Hasil dari pengukuran ini menunjukkan bahwasannya ada penggunaan teknologi cloud computing yang dapat meningkatkan performa basis data distribusi. Namun, terdapat beberapa faktor yang mempengaruhi hal ini seperti ukuran data dan jumlah node yang mempengaruhi performa tersebut. Oleh karena itu, disarankan untuk menggunakan teknologi yang tepat sesuai dengan kebutuhan dan karakteristik basis data yang diimplementasikan. Penelitian ini memberikan kontribusi dalam meningkatkan pemahaman mengenai penggunaan teknologi cloud computing dalam meningkatkan performa basis data distribusi.
Negative Pressure Level and Effects on Bacterial Growth Kinetics in an in vitro Wound Model
Authors: Bobkiewicz Adam , Francuzik Wojciech , Martinkosky Amy , Borejsza-Wysocki Maciej , Ledwosinski Witold , Szmyt Krzysztof , Banasiewicz Tomasz , Krokowicz Lukasz 
Published: June 2024

Abstract
Negative Pressure Wound Therapy (NPWT) has been widely adopted in wound healing strategies due to its multimodal mechanism of action. While NPWT’s positive impression on wound healing is well-established, its effect on bacterial load reduction remains equivocal. This study investigates NPWT’s efficacy in reducing bioburden using an in vitro porcine skin model, focusing on the impact of Staphylococcus aureus and Staphylococcus epidermidis. Custom-made negative pressure chambers were employed to apply varying negative pressures. Porcine skin was cut into 5 × 5 cm squares and three standardized wounds of 6 mm each were created using a biopsy punch. Then, wounds were infected with S. aureus and S. epidermidis bacterial suspensions diluted 1:10,000 to obtain a final concentration of 1.5 × 104 CFU/ml and were placed in negative pressure chambers. After incubation, bacterial counts were expressed as colony-forming units (CFU) per ml. For S. aureus at 120 hours, the median CFU, mean area per colony, and total growth area were notably lower at −80 mmHg when compared to −250 mmHg and −50 mmHg, suggesting an optimal negative pressure for the pressure-dependent inhibition of the bacterial proliferation. While analyzing S. epidermidis at 120 hours, the response to the negative pressure was similar but less clear, with the minor CFU at −100 mmHg. The influence of intermittent negative pressure on the S. epidermidis growth showed notably lower median CFU with the interval therapy every hour compared to the S. aureus control group. This study contributes valuable insights into NPWT’s influence on the bacterial load, emphasizing the need for further research to reformulate its role in managing contaminated wounds.
Halogenated Secondary Metabolites from Higher Plants: Potent Drug Candidates for Chikungunya Using in silico Approaches
Authors: Kumar Sushil , Joshi Nidhi , Choudhir Gourav , Sharma Sakshi , Tiwari Abhay , Alharbi Sulaiman Ali , Alfarraj Saleh , Ansari Mohammad Javed 
Published: June 2024

Abstract
Chikungunya virus (CHIKV) causes a debilitating fever and joint pain, with no specific antiviral treatment available. Halogenated secondary metabolites from plants are a promising new class of drug candidates against chikungunya, with unique properties that make them effective against the virus. Plants produce these compounds to defend themselves against pests and pathogens, and they are effective against a wide range of viruses, including chikungunya. This study investigated the interactions of halogenated secondary metabolites with nsP2pro, a therapeutic target for CHIKV. A library of sixty-six halogenated plant metabolites screened previously for ADME properties was used. Metabolites without violation of Lipinski’s rule were docked with nsP2pro using AutoDock Vina. To find the stability of the pipoxide chlorohydrin-nsP2pro complex, the GROMACS suite was used for MD simulation. The binding free energy of the ligand-protein complex was computed using MMPBSA. Molecular docking studies revealed that halogenated metabolites interact with nsP2pro, suggesting they are possible inhibitors. Pipoxide chlorohydrin showed the greatest affinity to the target. This was further confirmed by the MD simulations, surface accessible area, and MMPBSA studies. Pipoxide chlorohydrin, a halogenated metabolite, was the most potent against nsP2pro in the survey.
Guanylate-Binding Protein 1 (GBP1) Enhances IFN-α Mediated Antiviral Activity against Hepatitis B Virus Infection
Authors: Li Yadi , Luo Haiying , Hu Xiaoxia , Gong Jiaojiao , Tan Guili , Luo Huating , Wang Rui , Pang Hao , Yu Renjie , Qin Bo 
Published: June 2024

Abstract
Interferon-alpha (IFN-α) is a first-line drug for treating chronic hepatitis B (CHB). Guanylate-binding protein 1 (GBP1) is one of the interferon-stimulating factors, which participates in the innate immunity of the host and plays an antiviral and antibacterial role. In this study, we explored how GBP1 is involved in IFN-α antiviral activity against HBV. Before being gathered, HepG2-NTCP and HepG2 2.15 cells were transfected with the wild-type hGBP1 plasmid or si-GBP1, respectively, and followed by stimulation with Peg-IFNα-2b. We systematically explored the role of GBP1 in regulating HBV infection in cell models. Additionally, we also examined GBP1 levels in CHB patients. GBP1 activity increased, and its half-life was prolonged after HBV infection. Overexpression of GBP1 inhibited the production of HBsAg and HBeAg, as well as HBs protein and HBV total RNA levels, whereas silencing of GBP1 inhibited its ability to block viral infections. Interestingly, overexpressing GBP1 co-treatment with Peg-IFNα-2b further increased the antiviral effect of IFN-α, while GBP1 silencing co-treatment with Peg-IFNα-2b partly restored its inhibitory effect on HBV. Mechanistically, GBP1 mediates the anti-HBV response of Peg-IFNα-2b by targeting HBs. Analysis of clinical samples revealed that GBP1 was elevated in CHB patients and increased with Peg-IFNα-2b treatment, while GBP1 showed good stability in the interferon response group. Our study demonstrates that GBP1 inhibits HBV replication and promotes HBsAg clearance. It is possible to achieve antiviral effects through the regulation of IFN-α induced immune responses in response to HBV.
Establishment of a Nucleic Acid Detection Method for Norovirus GII.2 Genotype Based on RT-RPA and CRISPR/Cas12a-LFS
Authors: Wang Ting , Zeng Hao , Kang Jie , Lei Lanlan , Liu Jing , Zheng Yuhong , Qian Weidong , Fan Cheng 
Published: June 2024

Abstract
To establish a rapid detection method for norovirus GII.2 genotype, this study employed reverse transcription recombinase polymerase amplification (RT-RPA) combined with CRISPR/Cas12a and lateral flow strip (RT-RPA-Cas12a-LFS). Here, the genome of norovirus GII.2 genotype was compared to identify highly conserved sequences, facilitating the design of RT-RPA primers and crRNA specific to the conserved regions of norovirus GII.2. Subsequently, the reaction parameters of RT-RPA were optimized and evaluated using agar-gel electrophoresis and LFS. The results indicate that the conserved sequences of norovirus GII.2 were successfully amplified through RT-RPA at 37°C for 25 minutes. Additionally, CRISPR/Cas12a-mediated cleavage detection was achieved through LFS at 37°C within 10 minutes using the amplification products as templates. Including the isothermal amplification reaction time, the total time is 35 minutes. The established RT-RPA-Cas12a-LFS method demonstrated specific detection of norovirus GII.2, yielding negative results for other viral genomes, and exhibited an excellent detection limit of 10 copies/μl. The RT-RPA-Cas12a-LFS method was further compared with qRT-PCR by analyzing 60 food-contaminated samples. The positive conformity rate was 100%, the negative conformity rate was 95.45%, and the overall conformity rate reached 98.33%. This detection method for norovirus GII.2 genotype is cost-effective, highly sensitive, specific, and easy to operate, offering a promising technical solution for field-based detection of the norovirus GII.2 genotype.
SOL, VENTO, SOMBRA E ÁGUA FRESCA: quais seriam as características desejadas para um bom espaço público?
Authors: Franciscon Anderson , Bovo Marcos Clair 
Published: June 2024

Ao longo da história, os espaços públicos exerceram e sofreram substanciais transformações funcionais e espaciais as quais são resultantes de fatores socioculturais, econômicos e ambientais. À medida que a sociedade evolui e adquire novos hábitos, faz com que os espaços públicos se tornem mais ou menos atrativos, ou ainda, ficam à mercê de significativas reformas espaciais. Espaços públicos idealizados no século XX e até mesmo no século XXI, em muitas ocasiões já não atendem à sociedade contemporânea, globalizada e em constantes mutações socioculturais. Na contemporaneidade, diferentes espaços públicos apresentam diferentes intensidades quanto à capacidade de atrair e manter seus frequentadores, característica intrinsecamente ligadas aos seus atrativos (forma e função) e suas potencialidades. Nesse contexto, entendemos que os espaços públicos apresentam diferentes potenciais, sendo assim, temos como objetivo: caracterizar as potencialidades desejadas pela sociedade contemporânea por bons espaços públicos, considerando aspectos relativos à sua forma e função. Por fim, compreendemos que espaço público potencial é aquele feito para atender ao homem, fornecendo-lhe prazer, conforto e proteção, por meio de seu significado de legibilidade, diversidade, escala humana, espírito de vizinhança e flexibilidade funcional.
PENGARUH KONSENTRASI DAN FREKUENSI APLIKASI PUPUK ORGANIK CAIR TERHADAP PERTUMBUHAN DAN PRODUKSI TANAMAN KUBIS (Brasicca oleracea var. capitata)
Authors: Numba Sudirman , Robbo Anwar , Rahman K. Abd. 
Published: June 2024

Liquid Organic Fertilizer is a plant fertilizer that comes from organic materials. Raw materials come from wet organic materials or organic materials that have a high water content such as fruit and vegetable waste. POC has a good long-term effect on the soil, namely, it can improve the structure and organic content of the soil, and produce agricultural products that are safe for health so that POC can be used as environmentally friendly fertilizer. The research was carried out from August to October 2020, in Pasa' Dalle Tongko Village, Baroko District, Enrekang Regency. The research aims to determine the best concentration and frequency of POC treatment for the growth and production of cabbage plants. The research was prepared using a factorial design consisting of 2 factors. The first factor is the concentration of POC fertilizer with 3 levels, namely 1 mL/ L of water, 2 ml/ L of water, and 3 mL/ L of water. The second factor is the frequency of POC treatment which consists of 3 levels, namely 2 times spraying, 4 times spraying,and 6 times spraying. The results of the research showed that the interaction of various concentrations and frequency of POC spraying had no significant effect on all parameters observed. POC concentration of 3 mL/ L of water gave significantly different effects on all observation parameters, except for plant height. Similarly, the frequency of spraying 4 times gave a significantly different effect on all observation parameters
PEMANFAATAN BERBAGAI JENIS MIKROORGANISME LOKAL (MOL) SEBAGAI BIOAKTIVATOR PADA PENGOMPOSAN SAMPAH RUMAH TANGGA
Authors: Alimuddin Suraedah , Sabahannur St. , Syam Netty 
Published: June 2024

Nationally, the highest source of waste is generated from households, namely 40.38%, while household waste is dominated by organic waste. Total waste production in Makassar City will reach 373,653.9 tons/year in 2021 or the equivalent of 0.72 kg/person/day. Efforts to overcome the high production of waste require processing waste into useful products such as compost. The addition of bioactivators such as local microorganisms can speed up the composting process. The research aimed to test various types of MOL on the quality of compost from household waste. This research was carried out using a Completely Randomized Design (CRD) with the treatment of several types of MOL, namely: tempeh MOL, rice MOL, fruit MOL, bamboo shoot MOL, and EM-4 as a comparison. The parameters observed were 1) type and density of microbes in MOL, 2) physical properties of compost: temperature, color, aroma, and texture, and 3) chemical properties of compost: levels of N, P, K, C-org, C/N, pH. The results showed that there were variations in the types of microbes in the MOL made and rice MOL had the highest microbial density, namely 8.36 x 107. Tempe MOL produced the highest compost N content (0.70%) while rice MOL produced P content (1.70% ) and K (1.84%) is the highest. Rice MOL also produces the highest N+P+K levels (3.71%) compared to other types of MOL. All types of MOL that were tried produced physical quality (color, aroma, and texture) and chemical quality of compost that met the requirements of SNI compost 19-7030-2004 except for the C-organic content of the compost
Examining the influence of body fat distribution on standing balance and functional performance in overweight female patients with degenerative lumbar disease
Authors: Chen Jung-Hsuan , Chen Po-Jung , Kantha Phunsuk , Tsai Yi-Ching , Lai Dar-Ming , Hsu Wei-Li 
Published: June 2024

Introduction:Degenerative lumbar disease (DLD) is a prevalent disorder that predominantly affects the elderly population, especially female. Extensive research has demonstrated that overweight individuals (categorized by body fat distribution) have a higher susceptibility to developing DLD and an increased risk of falling. However, there is limited research available on the standing balance and functional performance of overweight females with DLD.Aims:To determine the impact of body fat distribution on standing balance and functional performance in overweight females with DLD.Methods:This cross-sectional study evaluated thirty females with DLD were categorized into three types of body fat distribution based on body mass index (BMI) and waist-hip ratio, specifically as android-type, gynoid-type, and normal weight groups. In addition, a control group of ten age-matched females with normal weight was recruited. The Visual Analogue Scale, Roland Morris Disability Questionnaire, Cobb angle (Determined using x-ray), and body composition (Determined using the InBody S10), were conducted only on the DLD groups. All participants were assessed standing balance in the anteroposterior and mediolateral directions. The functional assessments included timed-up-and-go and 5-times-sit-to-stand tests.Results:There were 10 people in each group. Android-type (Age = 65.00 ± 6.34 years; BMI = 26.87 ± 2.05 kg/m2), Gynoid-type (Age = 65.60 ± 4.99 years; BMI = 26.60 ± 1.75 kg/m2), Normal weight (Age = 65.70 ± 5.92 years; BMI = 22.35 ± 1.26 kg/m2), and Control (Age = 65.00 ± 5.23 years; BMI = 22.60 ± 1.12 kg/m2). The android-type group had higher body fat, visceral fat, and lower muscle mass (p&lt; 0.05), along with an increased Cobb angle (p&lt; 0.05). They showed greater ellipse area, total excursion, and mean distance in the anteroposterior direction (p&lt; 0.05). During the functional performance assessments, the android-type group had longer durations in both the 5-times-sit-to-stand and timed-up-and-go tasks (p&lt; 0.05).Conclusion:Our study found that android-type overweight individuals showed postural instability, reduced functional performance, and insufficient lower limb muscle strength and mass. These findings might help physical therapists in planning interventions, as they imply that patients with DLD may require specific types of standing balance training and lower extremities muscle-strengthening based on their body fat distribution.Clinical Trial Registration:ClinicalTrials.gov, identifier NCT05375201
Characterization of the somatostatin system in tilapia: implications for growth and reproduction
Authors: Mizrahi Naama , Hollander-Cohen Lian , Atre Ishwar , Shulman Miriam , Campo Aurora , Levavi-Sivan Berta 
Published: June 2024

Somatostatin (SST) plays diverse physiological roles in vertebrates, particularly in regulating growth hormone secretion from the pituitary. While the function of SST as a neuromodulator has been studied extensively, its role in fish and mammalian reproduction remains poorly understood. To address this gap, we investigated the involvement of the somatostatin system in the regulation of growth and reproductive hormones in tilapia. RNA sequencing of mature tilapia brain tissue revealed the presence of three SST peptides: SST6, SST3, and low levels of SST1. Four different isoforms of the somatostatin receptor (SSTR) subfamily were also identified in the tilapia genome. Phylogenetic and synteny analysis identified tiSSTR2-like as the root of the tree, forming two mega clades, with SSTR1 and SSTR4 in one and SSTR2a, SSTR3a, and SSTR5b in the other. Interestingly, the tiSSTR-5 isoforms 5x1, 5x2, and 5x3 were encoded in the sstr3b gene and were an artifact of misperception in the nomenclature in the database. RNA-seq of separated pituitary cell populations showed that SSTRs were expressed in gonadotrophs, with sstr3a enriched in luteinizing hormone (LH) cells and sstr3b significantly enriched in follicle-stimulating hormone (FSH) cells. Notably, cyclosomatostatin, an SSTR antagonist, induced cAMP activity in all SSTRs, with SSTR3a displaying the highest response, whereas octreotide, an SSTR agonist, showed a binding profile like that observed in human receptors. Binding site analysis of tiSSTRs from tilapia pituitary cells revealed the presence of canonical binding sites characteristic of peptide-binding class A G-protein-coupled receptors. Based on these findings, we explored the effect of somatostatin on gonadotropin release from the pituitary in vivo. Whereas cyclosomatostatin increased LH and FSH plasma levels at 2 h post-injection, octreotide decreased FSH levels after 2 h, but the LH levels remained unaffected. Overall, our findings provide important insights into the somatostatin system and its mechanisms of action, indicating a potential role in regulating growth and reproductive hormones. Further studies of the complex interplay between SST, its receptors, and reproductive hormones may advance reproductive control and management in cultured populations.
Pro-inflammatory diets promote the formation of hyperuricemia
Authors: Liu Xin , Chen Ting-Yu , Gao Teng-Yu , Shi Ke-Qin , Yin Fu-Qiang , Yu Yun-Xiang , Zhang Chao 
Published: June 2024

BackgroundHyperuricemia, as a very prevalent chronic metabolic disease with increasing prevalence year by year, poses a significant burden on individual patients as well as on the global health care and disease burden, and there is growing evidence that it is associated with other underlying diseases such as hypertension and cardiovascular disease. The association between hyperuricemia and dietary inflammatory index (DII) scores was investigated in this study.MethodsThis study enrolled 13, 040 adult subjects (aged ≥ 20 years) from the US National Health and Nutrition Survey from 2003 to 2018. The inflammatory potential of the diet was assessed by the DII score, and logistic regression was performed to evaluate the relationship between the DII score and the development of hyperuricemia; subgroup analyses were used to discuss the influence of other factors on the relationship.ResultsParticipants in the other quartiles had an increased risk of hyperuricemia compared to those in the lowest quartile of DII scores. Stratification analyses stratified by body mass index (BMI), sex, hypertension, drinking, diabetes, education level and albumin-creatinine-ratio (ACR) revealed that the DII score was also associated with the risk of hyperuricemia (P&lt;0.05). There was an interaction in subgroup analysis stratified by sex, age, and hypertension (P for interaction &lt;0.05). The results showed a linear-like relationship between DII and hyperuricemia, with a relatively low risk of developing hyperuricemia at lower DII scores and an increased risk of developing hyperuricemia as DII scores increased.ConclusionsThis study showed that the risk of hyperuricemia increased at slightly higher DII scores (i.e., with pro-inflammatory diets), but not significantly at lower levels (i.e., with anti-inflammatory diets). The contribution of the DII score to the development of hyperuricemia increased with higher scores. The relationship between inflammatory diets and hyperuricemia requires more research on inflammation, and this study alerts the public that pro-inflammatory diets may increase the risk of developing hyperuricemia.