bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long-Lasting Persistence of Integrase Resistance Mutations in HIV-2-Infected Patients after Raltegravir Withdrawal
Authors:   the French ANRS HIV-2 Cohort (ANRS CO 05), Charpentier Charlotte , Larrouy Lucile , Matheron Sophie , Damond Florence , Delelis Olivier , Mouscadet Jean-François , Campa Pauline , Chêne Geneviève , Brun-Vézinet Françoise , Descamps Diane 
Published: June 2011

Background Little is known in HIV-2 infection about the kinetics of disappearance of raltegravir (RAL)-resistant virus after RAL withdrawal. Methods RAL was interrupted in four highly antiretroviral- experienced HIV-2-infected patients exhibiting a virological failure when receiving RAL. Integrase gene was sequenced from plasma samples collected at the time of RAL failure and at further time points following RAL withdrawal. Results At the time of RAL withdrawal, virus exhibited different integrase resistance pathways: G140S/Q148R, E92Q/N155H, T97A/N155H and T97A/Y143C. In patient 1, the G140S/Q148R double-mutant was still detected at month (M)7 and at M11 after stopping RAL, but was no longer detected at M15. Regarding patient 2, the double- mutant E92Q/N155H was still present at M2 and M8 after stopping RAL, and was no longer detected at M12. In patient 3, RAL-resistant virus with T97A/N155H mutations were still present 1 month after stopping RAL, and were no longer detected at M14. Regarding patient 4, the mutant T97A/Y143C was still detected at M1 and M3 following RAL withdrawal. At M18 after RAL stop, integrase genotypic pattern evolved to T97A/Y143G. Conclusions Persistence of HIV-2 RAL-resistant mutants was observed in all the key genetic RAL resistance pathways. These findings have clinical implications especially in HIV-2-infected patients for whom therapeutic arsenal is limited compared to HIV-1, since the persistence of resistant mutants might compromise the possible efficacy of upcoming second-generation integrase inhibitors.
Aux sources d'une notion (l'apport de Robert Pagès)
Authors: Maisonneuve Jean 
Published: June 2011

Alors que Freud et sa mouvance traitent de « pulsion d’emprise » dans la perspective strictement psychanalytique, Robert Pagès, sans la récuser, met la notion des processus d’emprise au centre de la psychologie sociale ; il produit articles et colloques sur ce thème. Sans éviter une certaine exubérance lexicale, il confère à l’emprise un sens et un champ extensif et compréhensif très stimulants. Il mène parallèlement, des expérimentations, dont plusieurs résultats convergent avec les acquis cliniques, notamment sur l’impact de la formation relationnelle.
Emprise, résistance et dégagement : réflexions à partir des révolutions tunisienne, égyptienne, etc.
Authors: Laoukili Abdelaâli 
Published: June 2011

Cet article propose d’analyser les deux révolutions tunisienne et égyptienne selon deux points de vue : le premier est celui des mécanismes d’emprise à l’œuvre entre dirigeants et citoyens dans ces deux pays et un peu partout dans les pays anciennement colonisés. Le second est celui des mécanismes d’emprise qui ont lieu dans le rapport de l’Occident à ces pays. Pour le premier angle, nous développons le concept d’emprise du désir et, pour le second, celui de désir d’emprise. Nous terminons enfin par une mise en exergue des conditions et mécanismes d’émancipation et de dégagement de l’emprise.
Postmodernité et nouvelles formes du narcissisme chez le bébé
Authors: Mellier Denis 
Published: June 2011

L’auteur développe l’hypothèse que l’approfondissement de la modernité à un impact sur les représentations du « bébé parfait ». Au niveau culturel, la mise en évidence des compétences du bébé aboutit à l’individualiser de plus en plus tôt. Tandis que le matériel éducatif et de puériculture transmet cette influence, la pression sur la naissance, sa médicalisation et son entrée dans le champ marchand s’accompagnent d’une dé-ritualisation du temps du nourrisson. Les parents se trouvent paradoxalement plus seuls devant leur bébé. Ce défaut d’étayage social a des conséquences sur la différenciation des enveloppes psychiques tout au long de la petite enfance. Deux exemples cliniques illustrent cette thèse.
Bilateral Symmetric Autosomal Dominant Sector Chorioretinopathy with late Maculopathy: A Review Based on a Case with 48 Years Follow-up
Authors: Fledelius Hans C. , Rosenberg Thomas 
Published: June 2011

Purpose To describe the long-term course of bilateral symmetric autosomal dominant sector chorioretinopathy in a 79-year-old man who was diagnosed at age 31. Methods Ophthalmic examinations including fundus photography, fluorescein and indocyanine angiography, adaptometry, and ocular electrophysiology were performed at intervals from 1962 to 2010. Results and Conclusions The patient experienced no visual symptoms during his entire working life, but acquired an exudative maculopathy in his left eye at age 67. Two years later, a central subretinal neovascular membrane affected the right eye. The latter responded only temporarily to photodynamic therapy (Visudyne), and from age 71 he had best-corrected visual acuities <0.1 in both eyes, with eccentric fixation. We are not aware of any report on late macular involvement in this disorder. Whether the maculopathy should be considered a late manifestation of the sector chorioretinopathy or a coincidental occurrence of age-related macular degeneration remains unsettled.
Perfusion Circuit Concepts for Hollow-Fiber Bioreactors Used as in Vitro Cell Production Systems or Ex Vivo Bioartificial Organs
Authors: Balmert Stephen C. , McKeel Daniel , Triolo Fabio , Gridelli Bruno , Zeilinger Katrin , Bornemann Reiner , Gerlach Jörg C. 
Published: June 2011

For the development and implementation of primary human cell- and stem cell-based applications in regenerative medicine, large amounts of cells with well-defined characteristics are needed. Such cell quantities can be obtained with the use of hollow fiber-based bioreactors. While the use of such bioreactors generally requires a perfusion circuit, the configuration and complexity of such circuits is still in debate. We evaluated various circuit configurations to investigate potential perfusate volume shifts in the arterial and venous sides of the perfusion circuit, as well as in the feed and waste lines. Volume shifts with changes in flow conditions were measured with graduated bubble traps in the circuit, and perfusion pressures were measured at three points in the circuits. The results of this study demonstrate that the bioreactor perfusion circuit configuration has an effect on system pressures and volume shifts in the circuit. During operation, spikes in post-bioreactor pressures caused detrimental, potentially dangerous volume shifts in the feed and waste lines for configurations that lacked feed pumps and/or waste line check valves. Our results indicate that a more complex tubing circuit adds to safety of operation and avoids technical challenges associated with the use of large-scale hollow fiber bioreactors (e.g., for extracorporeal liver support or erythrocyte production from hematopoietic stem cells), including volume shifts and the need for a large reservoir. Finally, to ensure safe use of bioreactors, measuring pre-, intra-, and post-bioreactor pressures, and pump operation control is also advisable, which suggests the use of specifically developed bioreactor perfusion devices.
Melanoma-Associated Antigen (MAGE) Expression in the Normal Mucosa around Colorectal Cancer after Curative Resection: Presence of Undetectable Free Cancer Cells?
Authors: Jeon Chang-Ho , Kim Dae-Dong , Lee Han-Il , Oh Hoon-Kyu , Chae Hyun-Dong 
Published: June 2011

Purpose Curative surgical resection is of great importance and some trials have been performed to identify free undetectable cancer cells using molecular markers. The aim of this study is to investigate melanoma-associated antigen (MAGE) expression in normal mucosa around colorectal cancer and its clinical significance. Material and method From October 2003 to June 2004, we collected 46 colorectal cancer and matched normal mucosal tissues within 20 mm, 20 to 50 mm and more than 50 mm from tumors after the curative operation. Twenty-two mucosal tissues were harvested from patients with benign colorectal diseases as controls. MAGE expression was assayed using nested RT-PCR of MAGE A1-6 mRNA. Results The MAGE expression rates in cancer tissue and adjacent normal mucosa were 65.2%, 6.5% (<20 mm), 2.2% (20–50 mm) and 0.0% (>50 mm), respectively, while MAGE was not expressed in the mucosa of benign diseases. The MAGE-positive cases in the normal mucosa around tumors were located in the left colon or rectum, and one patient showed anastomotic mucosal site recurrence. Conclusions MAGE expression in normal-appearing mucosa around colorectal cancer showed some clinical findings suggesting the presence of undetectable free cancer cells after curative resection.
Coexpression of PDGFR-Alpha, PDGFR-Beta and VEGF as a Prognostic Factor in Patients with Hepatocellular Carcinoma
Authors: Chen Li , Shi Yan , Jiang Cheng-ying , Wei Li-xin , Lv Ya-li , Wang Yu-lan , Dai Guang-hai 
Published: June 2011

Aims To evaluate the prognostic value of vascular endothelial growth factor (VEGF), platelet-derived growth factor receptor-alpha (PDGFR-α) and beta (PDGFR-β) expression in patients with hepatocellular carcinoma (HCC). Methods The expression of PDGFR-α, PDGFR-β and VEGF in 63 HCC patients who underwent curative resection was examined by immunohistochemistry (IHC). The correlations between the expression of these biomarkers and the clinicopathological characteristics were analyzed. Patient survival was analyzed by univariate analysis and Cox proportional hazards model. Results Univariate survival analysis showed that PDGFR-α or PDGFR-β overexpression was of no prognostic significance in predicting disease-free survival (DFS) and overall survival (OS) (p>0.05), while VEGF overexpression and PDGFR-α/PDGFR-β/VEGF coexpression were significantly correlated with worse DFS and poorer OS in HCC patients (P<0.05). More importantly, PDGFR-α/PDGFR-β/VEGF coexpression was an independent prognostic marker for poor survival as indicated by multivariate Cox regression analysis (DFS, hazard ratio 3.122, p=0.001; OS, hazard ratio 4.260, p=0.000). Conclusions Coexpression of PDGFR-α, PDGFR-β and VEGF could be considered an independent prognostic biomarker for predicting DFS and OS in HCC patients. This result could be used to identify patients at a higher risk of tumor recurrence and poor prognosis, and help to select therapeutic schemes for the treatment of HCC.
RFID as a New ICT Tool to Monitor Specimen Life Cycle and Quality Control in a Biobank
Authors: Nanni Umberto , Spila Antonella , Riondino Silvia , Valente Maria Giovanna , Somma Paolo , Iacoboni Mauro , Alessandroni Jhessica , Papa Veronica , Della-Morte David , Palmirotta Raffaele , Ferroni Patrizia , Roselli Mario , Guadagni Fiorella 
Published: June 2011

Background Biospecimen quality is crucial for clinical and translational research and its loss is one of the main obstacles to experimental activities. Beside the quality of samples, preanalytical variations render the results derived from specimens of different biobanks or even within the same biobank incomparable. Specimens collected along the years should be managed with a heterogeneous life cycle. Hence, we propose to collect detailed data concerning the whole life cycle of stored samples employing radio-frequency identification (RFID) technology. Methods We describe the processing chain of blood biosamples that is operative at the biobank of IRCSS San Raffaele, Rome, Italy (BioBIM). We focus on the problem of tracing the stages following automated preanalytical processing: we collected the time stamps of all events that could affect the biological quality of the specimens by means of RFID tags and readers. Results We developed a pilot study on a fragment of the life cycle, namely the storage between the end of the preanalytics and the beginning of the analytics, which is usually not traced by automated tools because it typically includes manual handling. By adopting RFID devices we identified the possible critical time delays. At 1, 3 and 6 months RFID-tagged specimens cryopreserved at -80°C were successfully read. Conclusions We were able to record detailed information about the storage phases and a fully documented specimen life cycle. This will allow us to promote and tune up the best practices in biobanking because i) it will be possible to classify sample features with a sharper resolution, which allows future utilization of stored material; ii) cost-effective policies can be adopted in processing, storing and selecting specimens; iii) after using each aliquot, we can study the life cycle of the specimen with a possible feedback on the procedures.
Chromogranin a Measurement in Metastatic Well-Differentiated Gastroenteropancreatic Neuroendocrine Carcinoma: Screening for False Positives and a Prospective Follow-Up Study
Authors: Vezzosi Delphine , Walter Thomas , Laplanche Agnès , Raoul Jean Luc , Dromain Clarisse , Ruszniewski Philippe , d'Herbomez Michèle , Guigay Joël , Mitry Emmanuel , Cadiot Guillaume , Leboulleux Sophie , Lombard-Bohas Catherine , Borson-Chazot Françoise , Ducreux Michel , Baudin Eric ,   for the Groupe d'Etude des Tumeurs Endocrines (GTE)
Published: June 2011

Background Multiple causes of false-positive chromogranin A (CgA) measurement have been reported that may affect its impact as a surrogate marker of RECIST progression in well-differentiated gastroenteropancreatic neuroendocrine tumors (WDGEPNET). Aims 1) To evaluate the frequency of false-positive CgA results. 2) To prospectively compare CgA variations with RECIST morphological changes in patients without known causes of false-positive CgA measurements. Methods First, the conditions responsible for potentially false-positive CgA measurements were screened in 184 consecutive patients with metastatic WDGEPNET. Secondly, a variation in CgA at a 6-month interval was compared to RECIST results at 6 months in 46 patients. Results Among 184 patients, elevated CgA was found in 130 cases (71%) including 99 patients with at least one cause of a false-positive result. Impaired kidney function as well as medication with proton pump inhibitors were found to be the 2 major causes of false-positive results. The sensitivity and specificity of CgA measurements compared with morphological tumor changes according to the RECIST criteria were 71% and 50%, respectively, at 6 months. Conclusion Routine screening for the causes of false-positive CgA measurements is mandatory in WDGEPNET patients. Our study does not validate the use of CgA as a surrogate marker of tumor progression.
Two Novel Polymorphisms in 5' Flanking Region of the Mesothelin Gene are Associated with Soluble Mesothelin-Related Peptide (SMRP) Levels
Authors: Cristaudo Alfonso , Foddis Rudy , Bonotti Alessandra , Simonini Silvia , Vivaldi Agnese , Guglielmi Giovanni , Bruno Rossella , Gemignani Federica , Landi Stefano 
Published: June 2011

Background and aims Increased concentrations of soluble mesothelin-related peptides (SMRP) have been found in sera of patients with malignant pleural mesothelioma (MPM) even if a relatively high rate of false positives has hampered their clinical use as a tumor marker. Individual SMRP levels could be affected by polymorphic elements. The aim of this study was to investigate the association between single nucleotide polymorphisms within the promoter-5'UTR regions and SMRP levels in healthy asbestos-exposed individuals and patients suffering from MPM. Methods The promoter-5'UTR regions of the mesothelin gene were genotyped in 59 healthy asbestos-exposed subjects and 27 MPM patients. SMRP levels were measured using a commercially available ELISA kit. Results Two novel polymorphisms, an A>C variant (called New1) and a C>T variant (called New2), were identified. In healthy subjects, high SMRP levels were associated with the C-variant of New1, with an average 1.62-fold increase compared with AA homozygotes (p<0.0001). Most of the C-allele carriers had SMRP levels above the threshold of 1.00 nM. We set two different SMRP cutoffs on the basis of the combined New1+New2 genotypes. Conclusions New1-New2 genotypes could be employed as markers for setting individualized and appropriate thresholds of “normality” when SMRP is used in surveillance programs of asbestos-exposed people.
TRAIN: Training through Research Application Italian Initiative
Authors: Lombardo Claudio , Bottero Sergio , d'Alessandro Francesca , Giacomini Mauro , Guderzo Angela , Moretti Franca , Marincola Margherita , Pesce Giorgia , Pierotti Marco A. , Spagnoli Luigi Giusto , Belardelli Filippo 
Published: June 2011

Training through Research Application Italian iNitiative (TRAIN) is a mobility program financed under the EU action called “Cofinancing of regional, national and international programs” (COFUND) of the European Commission Seventh Framework Program (FP7) – People, and has been designed to encourage the promotion and development of international programs of research through mobility at various stages of research careers. The aim of TRAIN is to improve translational skills in the field of cancer by promoting a three-year international mobility program assigning a total of 51 fellowships subdivided into incoming, outgoing and reintegration fellowships. The TRAIN proposal has been submitted in February 2009 to the European Commission in reply to the 2008 FP7-PEOPLECOFUND call and has been successfully evaluated. TRAIN is addressed to postdoctoral scientists or scientists who have at least four years' full-time equivalent research experience and who wish to improve their careers spending one year abroad. The mobility program is open also to non-Italian experienced scientists wishing to spend one year in an Italian research center or private company. Part of the scheme is targeted to experienced Italian scientists who have completed at least three years of research in a foreign country and are interested in returning to Italy. TRAIN is part of an overall Italian strategy outlined by the International Program of the Italian Cancer Network “Alleanza Contro il Cancro” to promote Italian participation in the building of the European Area for translational cancer research and to enhance the interaction between academy and industry.
Independent Prediction Factors for Primary Patency Loss in Arteriovenous Grafts within Six Months
Authors: Monroy-Cuadros Mauricio , Yilmaz Serdar , Salazar-Bañuelos Anastasio , Doig Christopher 
Published: June 2011

Purpose The objective of this study was to explore the association between loss of primary functional patency within 6 months of first use and demographic and clinical characteristics in patients with arteriovenous grafts (AVGs) receiving chronic hemodialysis. The knowledge and management of these characteristics will minimize the proportion of catheter-dependent dialysis patients for whom AVGs are the best choice. Methods This was a retrospective study of all chronic hemodialysis patients with AVGs followed by the Southern Alberta Renal Program from January 2005 to June 2008. Demographic and clinical variables and initial intra-access blood flow (IABF) were compared between those with and without loss of primary functional patency. To determine the contribution of independent variables to the dependant variable of loss of primary functional patency, a multivariable analysis using logistic regression was performed. Results The incidence of primary failure was 30% (107/359). Multivariable analysis found that low initial IABF (<650 mL/min, odds ratio [OR] 31, P < 0.001), presence of diabetes (OR 3.5, P = 0.001), older age (>65 years OR 3.2, P< 0.001), and presence of peripheral vascular disease (OR 2.5, P< 0.005) were independently associated with loss of primary patency Conclusions AVGs are sometimes a better choice for those patients in which the time to and probability of successful fistula maturation may be a concern. Close monitoring of AVGs in patients with the identified risk factors associated with loss of primary patency may improve the life expectancy of the access.
Homogeneous Hydrogenations of Nitriles Catalyzed by Rhenium Complexes
Authors: Rajesh Kunjanpillai , Dudle Balz , Blacque Olivier , Berke Heinz 
Published: June 2011

AbstractRhenium(I) nitrosyl complexes bearing large bite angle diphosphines efficiently catalyze the hydrogenation of nitriles to the corresponding symmetrical secondary amines.
A Novel Assembly of Substituted Pyrroles by Acid‐Catalyzed Sequential Three‐Component Reaction of Amines, Alkynoates, and 1,2‐Diaza‐1,3‐dienes
Authors: Attanasi Orazio A. , Favi Gianfranco , Mantellini Fabio , Moscatelli Giada , Santeusanio Stefania 
Published: June 2011

AbstractA novel protocol for the assembly of polysubstituted pyrroles has been developed through the acid‐catalyzed, sequential three‐component reaction of primary aliphatic amines, alkynoates and 1,2‐diaza‐1,3‐dienes (DDs). This methodology proceeds with complete chemo‐/regioselectivity involving first formation of an enamino ester intermediate, in situ Michael addition with azo‐ene compounds and subsequent intramolecular ring closure.
Flammability behavior of fiber–fiber hybrid fabrics and composites
Authors: Indu Shekar R. , Kotresh T. M. , Damodhara Rao P. M. , Satheesh Kumar M. N. , Siddaramaiah  , Rahman Md. S. 
Published: June 2011

AbstractThe effect of heat flux levels on burning behavior and heat transmission properties of hybrid fabrics and composites has been investigated using cone calorimeter and heat transmission techniques. The hybrid fabric structures woven out of E‐glass (warp) and polyether ether ketone (PEEK) (weft) and E‐glass (warp) and polyester (weft) have been studied at high heat flux levels keeping in view the flame retardant requirements of structural composites. The performance of the glass–PEEK fabric even at high heat flux levels of 75 kW/m2 was comparable with the performance of glass–polyester fabric evaluated at 50 kW/m2. The results further demonstrate that glass–PEEK hybrid fabrics exhibit low peak heat release rate, low heat release rate, low heat of combustion, suggesting an excellent combination of materials and fall under the low‐risk category and are comparable with the performance of carbon fiber‐epoxy‐based systems. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Chemically cross‐linked chitosan hydrogel loaded with gelatin for chondrocyte encapsulation
Authors: Hu Xiaohong , Li Dan , Gao Changyou 
Published: June 2011

AbstractComposite hydrogels can be used as a scaffolding material for chondrogenesis, which requires a biomimetic environment to maintain chondrocyte morphology and phenotype. In this study, gelatin molecules were loaded into a hydrogel polymerized from a chitosan derivative (CML) to form a semi‐interpenetrating polymer network. While the porous structure of the hydrogels in the dry state was not dependent on the gelatin content, the collapse extent and pore size decreased as the gelatin content increased. The gelatin loading also reduced the swelling ratio of the CML hydrogel and enhanced the hydrogel strength at 20°C due to gelation of the gelatin. The release behavior of the gelatin from the CML hydrogel could be controlled by many factors, such as the amount of gelatin, temperature, and solution pH. The weight loss of the composite hydrogel was expedited after gelatin loading and showed a positive relationship with the gelatin content. The results of in vitro cell culture in the hydrogels revealed that gelatin loading improved cell viability and promoted proliferation and glycosaminoglycans secretion of chondrocytes. This new scaffold production technology for chondrocyte encapsulation provides a further step towards CML applications in tissue engineering and other biomedical areas.