bluuebunny/crossref_metadata_2025_split huggingface dataset:
OECD validation of phase‐3 Hershberger assay using the stimulated weanling male rat in Korea
Authors: Moon Hyun‐Ju , Kang Tae Seok , Kim Tae Sung , Kang Il Hyun , Kim Seung Hee , Han Soon Young 
Published: January 2010

AbstractThe OECD has proposed a new, validated test guideline with the stimulated weanling male Hershberger assay to avoid the surgical castration step. In the present study, we assessed the relevance and reliability of the stimulated weanling Hershberger assay in four stages. All chemicals except for testosterone propionate (TP) were orally administered to sexually immature male rats of 22 days old for 10 days. The weights of four mandatory accessory sex organs, two additional reproductive tissues and optional systemic organs were evaluated. At the first two stages, TP, as reference androgen, significantly increased the weights of epididymides and accessory sex organs (ASO) at 1.0 mg kg−1and flutamide (FLU), as a positive anti‐androgen control, decreased the TP‐stimulated organ weights at 3.0 mg kg−1. At stage 3, trenbolone (40 mg kg−1), an anabolic steroid, significantly increased ASO weights, and weak anti‐androgens (DDE and linuron) decreased the TP‐stimulated ASO weights at each high dose. The above results were confirmed in a blind test with coded substances provided by OECD. Compared with results from our previous castrated male assay, the intact weanling version is less sensitive than the castrated male version, in terms of a smaller response at the reference dose of TP or FLU. However, this study suggests that the stimulated weanling Hershberger assay can detect the effects of both potent and weak anti‐androgens on androgen‐producing and androgen‐dependent tissues. Copyright © 2010 John Wiley & Sons, Ltd.
Quantum chemical modeling of enzymatic reactions: The case of histone lysine methyltransferase
Authors: Georgieva Polina , Himo Fahmi 
Published: January 2010

AbstractQuantum chemical cluster models of enzyme active sites are today an important and powerful tool in the study of various aspects of enzymatic reactivity. This methodology has been applied to a wide spectrum of reactions and many important mechanistic problems have been solved. Herein, we report a systematic study of the reaction mechanism of the histone lysine methyltransferase (HKMT) SET7/9 enzyme, which catalyzes the methylation of the N‐terminal histone tail of the chromatin structure. In this study, HKMT SET7/9 serves as a representative case to examine the modeling approach for the important class of methyl transfer enzymes. Active site models of different sizes are used to evaluate the methodology. In particular, the dependence of the calculated energies on the model size, the influence of the dielectric medium, and the particular choice of the dielectric constant are discussed. In addition, we examine the validity of some technical aspects, such as geometry optimization in solvent or with a large basis set, and the use of different density functional methods. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
TD‐DFT study on the sensing mechanism of a fluorescent chemosensor for fluoride: Excited‐state proton transfer
Authors: Li Guang‐Yue , Zhao Guang‐Jiu , Liu Yu‐Hui , Han Ke‐Li , He Guo‐Zhong 
Published: January 2010

AbstractAn excited‐state proton transfer (ESPT) process, induced by both intermolecular and intramolecular hydrogen‐bonding interactions, is proposed to account for the fluorescence sensing mechanism of a fluoride chemosensor, phenyl‐1H‐anthra(1,2‐d)imidazole‐6,11‐dione. The time‐dependent density functional theory (TD‐DFT) method has been applied to investigate the different electronic states. The present theoretical study of this chemosensor, as well as its anion and fluoride complex, has been conducted with a view to monitoring its structural and photophysical properties. The proton of the chemosensor can shift to fluoride in the ground state but transfers from the proton donor (NH group) to a proton acceptor (neighboring carbonyl group) in the first singlet excited state. This may explain the observed red shifts in the fluorescence spectra in the relevant fluorescent sensing mechanism. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
Reaction mechanism of methanol decomposition on Pt‐based model catalysts: A theoretical study
Authors: Niu Cui‐Yu , Jiao Jiao , Xing Bin , Wang Gui‐Chang , Bu Xian‐He 
Published: January 2010

AbstractThe decomposition mechanisms of methanol on five different Pt surfaces, the flat surface of Pt(111), Pt‐defect, Pt‐step, Pt(110)(1 × 1), and Pt(110)(2 × 1), have been studied with the DFT‐GGA method using the repeated slab model. The adsorption energies under the most stable configuration of the possible species and the activation energy barriers of the possible elementary reactions involved are obtained in this work. Through systematic calculations for the reaction mechanism of methanol decomposition on these surfaces, we found that such a reaction shows the same reaction mechanism on these Pt‐based model catalysts, that is, the final products are all H (Hads) and CO (COads) via OH bond breaking in methanol and CH bond scission in methoxy. These results are in general agreement with the previous experimental observations. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010.
Effects of genistein on β‐catenin signaling and subcellular distribution of actin‐binding proteins in human umbilical CD105‐positive stromal cells
Authors: Shieh Dar‐Bin , Li Rui‐You , Liao Jiuan‐Miaw , Chen Gin‐Den , Liou Ying‐Ming 
Published: January 2010

AbstractThis study was performed to define the roles of actin‐binding proteins in the regulation of actin filament assembly associated with cellular signal transduction pathways in stromal cell proliferation. Genistein, a tyrosine protein kinase inhibitor, decreased the intracellular Ca2+ and attenuated cell proliferation and DNA synthesis through the β‐catenin and cyclin D1 pathway in human umbilical CD105‐positive cells. Immunoprecipitation studies using anti‐β‐actin antibody revealed that several actin‐binding proteins implicated in cells include formin‐2 (FMN‐2), caldesmon (CaD), tropomyosin (Tm), and profilin. Protein levels of these proteins in whole cell lysates were not significantly changed by genistein. Three Tm isoforms, Tm‐1, Tm‐2, and Tm‐4, were found to be present in cells. Genistein caused a reduction in levels of mRNAs coding for Tm‐1 and Tm‐4, but had no significant effect on Tm‐2 mRNA levels. Immunofluorescence confocal scanning microscopy indicated that changes in the subcellular distribution of Tm and CaD, in which the diffuse cytosolic staining was shifted to show colocalization with actin stress fibers. In contrast, genistein‐induced accumulation of FMN‐2 and profilin in the peri‐nuclear area. Silencing of FMN‐2 by small interfering RNA resulted in increases of intracellular Ca2+ and rendered genistein resistance in decreasing intracellular Ca2+ in cells. These results provide the novel findings that genistein acts by modulating the cellular distribution of actin‐binding proteins in association with alterations of cellular signal transduction pathways in human stromal cell proliferation. J. Cell. Physiol. 223: 423–434, 2010. © 2010 Wiley‐Liss, Inc.
Modulation of coxsackie and adenovirus receptor expression for gene transfer to normal and dystrophic skeletal muscle
Authors: Larochelle Nancy , Teng Qingshan , Gilbert Rénald , Deol Jatinderpal R. , Karpati George , Holland Paul C. , Nalbantoglu Josephine 
Published: January 2010

AbstractBackgroundEfficient adenovirus (AdV)‐mediated gene transfer is possible only in immature muscle or regenerating muscle, suggesting that a developmentally regulated event plays a major role in limiting AdV uptake in mature skeletal muscle. Previously, we showed that the expression of the primary coxsackie and adenovirus receptor (CAR) is severely down‐regulated during muscle maturation and that, in muscle‐specific CAR transgenic mice, there is significant enhancement of AdV‐mediated gene transfer to mature skeletal muscle.MethodsTo evaluate whether increasing CAR expression can also augment gene transfer to dystrophic muscle that has many regenerating fibers, we crossed CAR transgenics with dystrophin‐deficient mice (mdx/CAR). We also tested a two‐step protocol in which CAR levels were increased in the target muscle, prior to administration of AdV, through the use of recombinant adeno‐associated virus (AAV2) expressing CAR. Lastly, we assessed the effect of histone deacetylase inhibitors on CAR and AdV transduction efficiency in myoblasts and mdx muscle.ResultsAlthough somewhat higher rates of transduction can be achieved in adult mdx mice than in normal mice as a result of ongoing muscle regeneration in these animals, CAR expression in the mdx background (mdx/CAR transgenics) still markedly improved the susceptibility of mature muscle to AdV‐mediated gene transfer of dystrophin. Prior administration of AAV2‐CAR to normal muscle led to significantly increased transduction by subsequent injection of AdV. The histone deacetylase inhibitor valproate increased CAR transcript and protein levels in myoblasts and mdx muscle, and also increased AdV‐mediated gene transfer.ConclusionsWe have developed a method of increasing CAR levels in both normal and regenerating muscle. Copyright © 2010 John Wiley & Sons, Ltd.
K4‐free graphs with no odd hole: Even pairs and the circular chromatic number
Authors: Zwols Yori 
Published: January 2010

AbstractAn odd hole in a graph is an induced cycle of odd length at least five. In this article we show that every imperfect K4‐free graph with no odd hole either is one of two basic graphs, or has an even pair or a clique cutset. We use this result to show that every K4‐free graph with no odd hole has circular chromatic number strictly smaller than 4. We also exhibit a sequence {Hn} of such graphs with limn→∞χc(Hn)=4. © 2010 Wiley Periodicals, Inc. J Graph Theory 65: 303–322, 2010
Large classes of infinite k‐cop‐win graphs
Authors: Bonato Anthony , Hahn Geňa , Tardif Claude 
Published: January 2010

AbstractWhile finite cop‐win finite graphs possess a good structural characterization, none is known for infinite cop‐win graphs. As evidence that such a characterization might not exist, we provide as large as possible classes of infinite graphs with finite cop number. More precisely, for each infinite cardinal κ and each positive integer k, we construct 2κ non‐isomorphic k‐cop‐win graphs satisfying additional properties such as vertex‐transitivity, or having universal endomorphism monoid and automorphism group. © 2010 Wiley Periodicals, Inc. J Graph Theory 65: 334–342, 2010
Biochemical characterization of an alkaline metallopeptidase secreted by a Pseudomonas fluorescens isolated from soil
Authors: Jankiewicz Urszula , Szawłowska Urszula , Sobańska Monika 
Published: January 2010

AbstractThe extracellular endopeptidase synthesized by soil bacterium Pseudomonas fluorescens was purified to homogeneity in a four‐step procedure. The enzyme was purified 45‐fold, with a 20% recovery. The endopeptidase appeared to be a monomer with a molecular mass of approx. 50 kDa. The enzyme was active in the pH range of 7 to 10. The optimal activity was detected at pH 9.0 and at 42 °C.Enzyme activity was inhibited by EDTA, EGTA and 1,10 phenanthroline, typical metalloprotease inhibitors. Ca2+ activated the enzyme while Zn2+, Co2+, Cd2+(in high concentration) strongly inhibited it. The presence of calcium ions strongly stabilized the enzyme with regard to thermal resistance. The amino acid sequence of fragments of the enzymatic protein determined by MS analysis revealed a high similarity to the sequences of other alkaline metalloendopeptidases of bacteria belonging to the genus Pseudomonas. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Estimation of combined effects of carbon and nitrogen sources on the growth and bacteriocin production of Lactobacillus salivarius from human source
Authors: Tomás María Silvina Juárez , Bru Elena , Nader‐Macías María Elena Fátima 
Published: January 2010

AbstractVaginal Lactobacillus salivarius CRL 1328 (Centro de Referencia para Lactobacilos Culture Collection) or the bacteriocin that produces could be included in a urogenital probiotic formula to prevent urogenital infections in women. The objective of this work was to determine the effects of different carbon and nitrogen sources on the growth and bacteriocin production of this microorganism. A fractional factorial design 35–1 was applied to evaluate the effects of five nutrients (glucose, lactose, yeast extract, tryptone and meat peptone), at three different concentrations (0, 1 and 2%). Results were statistically analyzed for linear and quadratic effects of nutrients, along with their interactions. All the nutrients tested stimulated the cell growth and bacteriocin production, but lactose had not a significant influence on the last response. The linear effects of higher magnitude on biomass and bacteriocin production were those of yeast extract and tryptone. The results demonstrated that there were significant interactions between the different nutrients, depending on the response evaluated. Maximum bacteriocin production was reached in different growth media with a lower cost than conventional culture media used in the laboratory. These findings will contribute to the design of a pharmaceutical product for the restoration of ecological balance of urogenital tract. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Gongronella sp induces overproduction of laccase in Panus rudis
Authors: Wei Fen , Hong Yuzhi , Liu Juanjuan , Yuan Jing , Fang Wei , Peng Hui , Xiao Yazhong 
Published: January 2010

AbstractLaccase is usually produced via chemical induction and is also synthesized by hosts in interaction with the typical bio‐control genus Trichoderma. In this study, we found that a newly isolated non‐laccase‐producing fungus, Gongronella sp. W5, could induce overproduction of laccase in Panus rudis. The enzyme activity, 148,200 U l–1, was 25 times higher than the activity obtained from a chemical induction using copper/o ‐toluidine as inducers. A new laccase isozyme from the interaction of P. rudis and G. W5 was purified and characterized. A further test showed that some pH resistant metabolites secreted by G. W5 acted as signals to induce P. rudis laccase. Laccase is also highly expressed by Trametes sp. AH28‐2 in interaction with Trichoderma sp. ZH1. However, no laccase activity was observed from the cross‐over interactions of P. rudis –Trichoderma sp. ZH1 or Trametes sp. AH28‐2–G. W5. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
PQQ‐Dependent Alcohol Dehydrogenase (QEDH) of Pseudomonas aeruginosa is involved in catabolism of acyclic terpenes
Authors: Chattopadhyay Ava , Förster‐Fromme Karin , Jendrossek Dieter 
Published: January 2010

AbstractGrowth of Pseudomonas aeruginosa on acyclic terpene alcohols such as geraniol depends on the presence of the atuRABCDEFGH gene cluster and a functional acyclic terpene utilisation (Atu) pathway. The proteins encoded by the atu gene cluster are necessary but not sufficient for growth on acyclic terpenes. Comparative 2‐dimensional polyacrylamide gel electrophoresis of soluble P. aeruginosa proteins revealed the presence of an additional spot (besides Atu proteins) that is specifically expressed in geraniol cells but is absent in isovalerate‐grown cells. The spot was identified as PA1982 gene product a pyrroloquinoline quinone (PQQ) dependent ethanol oxidoreductase (QEDH). Inactivation of PA1982 by insertion mutagenesis resulted in inability of the mutant to utilise ethanol and in reduced growth on geraniol. Growth on ethanol was restored by transferring an intact copy of the PA1982 gene into the mutant. The PA1982 gene product was purified from recombinant Escherichia coli and revealed PQQ‐dependent oxidoreductase activity with a variety of substrates including acyclic terpene derivates at comparable Vmax‐values. Our results show that QEDH participates in oxidation of acyclic terpene derivates in addition to the well‐known function in ethanol metabolism. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Improving the quality of processing gastric cancer specimens: The pathologist's perspective
Authors: Mahar Alyson L. , Qureshi Alia P. , Ottensmeyer C. Andrea , Chetty Runjan , Pollett Aaron , Coburn Natalie G. , Wright Frances C. 
Published: January 2010

AbstractBackground and ObjectivesResearch into surgeon and pathologist knowledge of guidelines for lymph node (LN) assessment in gastric cancer demonstrated a knowledge deficit. To understand factors affecting optimal assessment we surveyed pathologists to identify external barriers.MethodsPathologists were identified using two Ontario physician databases and surveyed online or by mail, with a 40% response rate.ResultsThe majority (56%) of pathologists stated assessing an additional five LNs would not be a burden. Most (80%) pathologists disagreed with pay for performance for achieving quality standards. Qualitative analysis determined the majority of pathologists believed achieving quality standards was inherent to their profession and should not require incentives. Poor surgical specimen was identified as a barrier and underscores the importance of aiming quality improvement initiatives at the multidisciplinary team.ConclusionIn addition to education, tailoring an intervention to address all barriers, including laboratory constraints may be an effective means of improving gastric cancer care. J. Surg. Oncol. 2010; 101:195–199. © 2010 Wiley‐Liss, Inc.
Secondary cytoreductive surgery in the management of platinum‐sensitive recurrent epithelial ovarian cancer
Authors: Park Jeong‐Yeol , Eom Jeong‐Min , Kim Dae‐Yeon , Kim Jong‐Hyeok , Kim Yong‐Man , Kim Young‐Tak , Nam Joo‐Hyun 
Published: January 2010

AbstractBackground and ObjectivesTo assess the surgical and survival outcomes after secondary cytoreductive surgery (SCRS) in patients with platinum‐sensitive recurrent epithelial ovarian cancer (EOC) and to identify patients who would most benefit from SCRS.MethodsInclusion criteria consisted of (1) recurrent EOC; (2) disease‐free interval (DFI) ≥6 months after primary therapy; and (3) SCRS with therapeutic intent followed by adjuvant chemotherapy.ResultsSixty‐seven patients met the inclusion criteria in the interval 1992–2008. Median DFI was 20 months (range: 6–109 months). Median time in surgery was 240 min (range: 60–680 min), and median estimated blood loss was 100 ml (range: 50–3,000 ml). There was no significant perioperative complication requiring reoperation. Complete resection of all visible tumor tissue was achieved in 37 patients (55.2%), with residual tumors (RT) ≤1 cm remaining in four patients (6%). After a mean follow‐up of 41 months (range: 6–145 months), the 2‐ and 5‐year disease‐free survival (DFS) rates were 32% and 10%, respectively, and the 2‐ and 5‐year overall survival (OS) rates were 58% and 26%, respectively. In multivariable analysis, DFI >24 months and RT ≤1 cm were significant predictors of DFS and OS.ConclusionsSCRS is safe and effective, with a low rate of complications. Patients who benefited most from this surgery were those with a DFI >24 months and patients who achieved optimal cytoreduction. J. Surg. Oncol. 2010; 101:418–424. © 2010 Wiley‐Liss, Inc.
Preoperative risk assessment and prevention of complications in patients with esophageal cancer
Authors: Grotenhuis Brechtje A. , Wijnhoven Bas P.L. , Grüne Frank , van Bommel Jasper , Tilanus Hugo W. , van Lanschot J. Jan B. 
Published: January 2010

AbstractIn this review the preoperative risk assessment and prevention of complications in patients undergoing esophagectomy for cancer is discussed. Age, pulmonary and cardiovascular condition, nutritional status, and neoadjuvant chemo(radio)therapy are known predictive factors. None of these factors is a valid exclusion criterion for esophagectomy, but may help in careful patient selection. Both anesthetists and surgeons play an important role in intraoperative risk reduction by means of appropriate fluid management and application of optimal surgical techniques. J. Surg. Oncol. 2010; 101:270–278. © 2010 Wiley‐Liss, Inc.
Hyper MV ‐ideals in hyper MV ‐algebras
Authors: Torkzadeh Lida , Ahadpanah Afsaneh 
Published: January 2010

AbstractIn this paper we define the hyper operations ⊗, ∨ and ∧ on a hyper MV ‐algebra and we obtain some related results. After that by considering the notions ofhyper MV ‐ideals and weak hyper MV ‐ideals, we prove some theorems. Then we determine relationships between (weak) hyper MV ‐ideals in a hyper MV ‐algebra (M, ⊕, *, 0) and (weak) hyper K ‐ideals in a hyper K ‐algebra (M, °, 0). Finally we give a characterization of hyper MV ‐algebras of order 3 or 4 based on the (weak) hyper MV ‐ideals (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
The expression of SIAH1 is downregulated and associated with Bim and apoptosis in human breast cancer tissues and cells
Authors: Wen Yuan‐Yuan , Yang Zhi‐Qiang , Song Min , Li Bai‐Lin , Yao Xiao‐Hui , Chen Xiao‐Ling , Zhao Jing , Lu Yi‐Yan , Zhu Ji‐Jiang , Wang En‐Hua 
Published: January 2010

AbstractSeven in absentia homolog1 (SIAH1) was reported as a tumor suppressor and played an important role in regulating cell apoptosis. However, its effects on the breast carcinogenesis remain unclear. In this study, our aims were to examine the relationship between SIAH1 and Bcl‐2‐interacting mediator of cell death (Bim) and to explore the effects of SIAH1 on the breast carcinogenesis. Immunohistochemical analysis in 231 cases of breast tissues showed that the expression of SIAH1 and Bim were significantly decreased in the breast carcinogenesis. Moreover, SIAH1 expression was significantly correlated with Bim. Both SIAH1 and Bim expression were significantly higher in well to moderately differentiated and in early‐stage breast cancer. Reverse transcription (RT)‐polymerase chain reaction (PCR) and Western blot analysis in paired breast cancer tissues and breast cell lines found that the expression of SIAH1 was lower in the breast cancer tissues and cell lines. SIAH1 inducing apoptosis of the breast cancer cells was dependent on Bim. However, SIAH1 inhibiting invasion of the breast cancer cells was independent of Bim. The increase of the function of SIAH1 to upregulate the expression of Bim may play an important role in the progression of breast cancer. Restoration of the function of SIAH1 may be a new therapeutic target of human breast cancer. © 2010 Wiley‐Liss, Inc.