bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experimental hyperprolinemia induces mild oxidative stress, metabolic changes, and tissue adaptation in rat liver
Authors: Ferreira Andréa G.K. , da Cunha Aline A. , Machado Fernanda R. , Pederzolli Carolina D. , Dalazen Giovana R. , de Assis Adriano M. , Lamers Marcelo L. , dos Santos Marinilce F. , Dutra‐Filho Carlos S. , Wyse Angela T.S. 
Published: September 2011

AbstractThe present study investigated the effects of chronic hyperprolinemia on oxidative and metabolic status in liver and serum of rats. Wistar rats received daily subcutaneous injections of proline from their 6th to 28th day of life. Twelve hours after the last injection the rats were sacrificed and liver and serum were collected. Results showed that hyperprolinemia induced a significant reduction in total antioxidant potential and thiobarbituric acid‐reactive substances. The activities of the antioxidant enzymes catalase and superoxide dismutase were significantly increased after chronic proline administration, while glutathione (GSH) peroxidase activity, dichlorofluorescin oxidation, GSH, sulfhydryl, and carbonyl content remained unaltered. Histological analyses of the liver revealed that proline treatment induced changes of the hepatic microarchitecture and increased the number of inflammatory cells and the glycogen content. Biochemical determination also demonstrated an increase in glycogen concentration, as well as a higher synthesis of glycogen in liver of hyperprolinemic rats. Regarding to hepatic metabolism, it was observed an increase on glucose oxidation and a decrease on lipid synthesis from glucose. However, hepatic lipid content and serum glucose levels were not changed. Proline administration did not alter the aminotransferases activities and serum markers of hepatic injury. Our findings suggest that hyperprolinemia alters the liver homeostasis possibly by induction of a mild degree of oxidative stress and metabolic changes. The hepatic alterations caused by proline probably do not implicate in substantial hepatic tissue damage, but rather demonstrate a process of adaptation of this tissue to oxidative stress. However, the biological significance of these findings requires additional investigation. J. Cell. Biochem. 113: 174–183, 2012. © 2011 Wiley Periodicals, Inc.
High Glucose‐induced repression of RAR/RXR in cardiomyocytes is mediated through oxidative stress/JNK signaling
Authors: Singh Amar B. , Guleria Rakeshwar S. , Nizamutdinova Irina T. , Baker Kenneth M. , Pan Jing 
Published: September 2011

AbstractThe biological actions of retinoids are mediated by nuclear retinoic acid receptors (RARs) and retinoid X receptors (RXRs). We have recently reported that decreased expression of RARα and RXRα has an important role in high glucose (HG)‐induced cardiomyocyte apoptosis. However, the regulatory mechanisms of HG effects on RARα and RXRα remain unclear. Using neonatal cardiomyocytes, we found that ligand‐induced promoter activity of RAR and RXR was significantly suppressed by HG. HG promoted protein destabilization and serine‐phosphorylation of RARα and RXRα. Proteasome inhibitor MG132 blocked the inhibitory effect of HG on RARα and RXRα. Inhibition of intracellular reactive oxidative species (ROS) abolished the HG effect. In contrast, H2O2 stimulation suppressed the expression and ligand‐induced promoter activity of RARα and RXRα. HG promoted phosphorylation of ERK1/2, JNK and p38 MAP kinases, which was abrogated by an ROS inhibitor. Inhibition of JNK, but not ERK and p38 activity, reversed HG effects on RARα and RXRα. Activation of JNK by over expressing MKK7 and MEKK1, resulted in significant downregulation of RARα and RXRα. Ligand‐induced promoter activity of RARα and RXRα was also suppressed by overexpression of MEKK1. HG‐induced cardiomyocyte apoptosis was potentiated by activation of JNK, and prevented by all‐trans retinoic acid and inhibition of JNK. Silencing the expression of RARα and RXRα activated the JNK pathway. In conclusion, HG‐induced oxidative stress and activation of the JNK pathway negatively regulated expression/activation of RAR and RXR. The impaired RAR/RXR signaling and oxidative stress/JNK pathway forms a vicious circle, which significantly contributes to hyperglycemia induced cardiomyocyte apoptosis. J. Cell. Physiol. 227: 2632–2644, 2012. © 2011 Wiley Periodicals, Inc.
Thyroid cysts treated with ethanol ablation can mimic malignancy during sonographic follow‐up
Authors: Park Noh Hyuck , Kim Dong Wook , Park Hee Jin , Lee Eun Ja , Park Ji Sung , Park Sung Il , Bae Jong Myon , Lee Jun Hwa 
Published: September 2011

AbstractPurpose.We aimed to assess long‐term ultrasound (US) findings after US‐guided percutaneous ethanol ablation (EA) in benign thyroid cysts and predominantly cystic thyroid nodules.Methods.Forty patients with thyroid cysts (n = 14) and predominantly cystic thyroid nodules (n = 26) underwent long‐term US follow‐up (range, 12–36 months; mean, 18.2 months) after EA. US images of 40 post‐EA nodules were retrospectively investigated to study the reduction in nodule volume and detailed US appearance.Results.On follow‐up US, post‐EA nodules showed the following features: Marked hypoechogenicity (n = 28), spiculated margin (n = 7), microcalcifications (n = 6), taller‐than‐wide shape (n = 2), centrally predominant vascularity (n = 3), no vascularity (n = 31), mixed vascularity (n = 3), and peripheral vascularity (n = 3). Post‐EA nodules were diagnosed by US as benign (n = 3), probably benign (n = 2), borderline (n = 5), possibly malignant (n = 20), and malignant (n = 10). No statistical difference in the incidence of malignant US findings was observed between thyroid cysts and predominantly cystic thyroid nodules (p > 0.05, Fisher's exact test).Conclusions.Long‐term follow‐up US after successful EA of benign thyroid cysts and predominantly cystic thyroid nodules revealed a high incidence of findings that are usually associated with malignancy. Recognizing these consequences of the procedure would help avoid unnecessary FNA on post‐EA nodules. © 2011 Wiley Periodicals, Inc. J Clin Ultrasound, 2011;
Impact of production practices on physicochemical properties of rice grain quality
Authors: Bryant Rolfe J , Anders Merle , McClung Anna 
Published: September 2011

AbstractBACKGROUND: Rice growers are interested in new technologies that can reduce input costs while maintaining high field yields and grain quality. The bed‐and‐furrow (BF) water management system benefits farmers through decreased water usage, labor, and fuel as compared to standard flood management. Fertilizer inputs can be reduced by producing rice in rotation with soybeans, a nitrogen‐fixing crop, and with the use of slow‐release fertilizers that reduce nitrogen volatilization and run‐off. However, the influence of these cultural management practices on rice physicochemical properties is unknown. Our objective was to evaluate the influence of nitrogen fertilizer source, water management system, and crop rotation on rice grain quality.RESULTS: Grain protein concentration was lower in a continuous rice production system than in a rice–soybean rotation. Neither amylose content nor gelatinization temperature was altered by fertilizer source, crop rotation, or water management. BF water management decreased peak and breakdown viscosities relative to a flooded system. Peak and final paste viscosities were decreased by all fertilizer sources, whereas, crop rotation had no influence on the Rapid Visco Analyser profile.CONCLUSION: Sustainable production systems that decrease water use and utilize crop rotations and slow‐release fertilizers have no major impact on rice physicochemical properties. Published 2011 by John Wiley & Sons, Ltd.
Bergman Cyclization in Polymer Chemistry and Material Science
Authors: Xiao Yuli , Hu Aiguo 
Published: September 2011

AbstractBergman cyclization of enediynes, regarded as a promising strategy for anticancer drugs, now finds its own niche in the area of polymer chemistry and material science. The highly reactive aromatic diradicals generated from Bergman cyclization can undergo polymerization acting as either monomers or initiators of other vinyl monomers. The former, namely homopolymerization, leads to polyphenylenes and polynaphthalenes with excellent thermal stability, good solubility, and processability. The many remarkable properties of these aromatic polymers have further endowed them to be manufactured into carbon‐rich materials, e.g., glassy carbons and carbon nanotubes. Whereas used as initiators, enediynes provide a novel resource for high molecular weight polymers with narrow polydispersities. The aromatic diradicals are also useful for introducing oligomers or polymers onto pristine carbonous nanomaterials, such as carbon nano‐onions and carbon nanotubes, to improve their dispersibility in organic solvents and polymer solutions.magnified image
Down‐regulation of connexin43 expression reveals the involvement of caveolin‐1 containing lipid rafts in human U251 glioblastoma cell invasion
Authors: Strale Pierre‐Olivier , Clarhaut Jonathan , Lamiche Coralie , Cronier Laurent , Mesnil Marc , Defamie Norah 
Published: September 2011

AbstractGlioblastoma cells are characterized by high proliferation and invasive capacities. Tumor development has been associated with a decrease of gap‐junctional intercellular communication, but the concrete involvement of gap junction proteins, connexins, remains elusive since they are also suspected to promote cell invasion. In order to better understand how connexins control the glioma cell phenotype, we studied the consequences of inhibiting the intrinsic expression of the major astrocytic connexin, Connexin43, in human U251 glioblastoma cells by the shRNA strategy. The induced down‐regulation of Cx43 expression has various effects on the U251 cells such as increased clonogenicity, angiogenesis and decreased adhesion on specific extracellular matrix proteins. We demonstrate that the invasion capacity measured in vitro and ex vivo correlates with Cx43 expression level. For the first time in a cancer cell context, our work demonstrates that Cx43 cofractionates, colocalizes and coimmunoprecipitates with a lipid raft marker, caveolin‐1 and that this interaction is inversely correlated to the level of Cx43. This localization of Cx43 in these lipid raft microdomains regulates both homo‐ and heterocellular gap junctional communications (respectively between U251 cells, or between U251 cells and astrocytes). Moreover, the adhesive and invasive capacities are not dependent, in our model, on Cav‐1 expression level. Our results tend to show that heterocellular gap junctional communication between cancer and stroma cells may affect the behavior of the tumor cells. Altogether, our data demonstrate that Cx43 controls the tumor phenotype of glioblastoma U251 cells and in particular, invasion capacity, through its localization in lipid rafts containing Cav‐1. © 2011 Wiley Periodicals, Inc.
Dietary lipids and their oxidized products in Alzheimer's disease
Authors: Corsinovi Laura , Biasi Fiorella , Poli Giuseppe , Leonarduzzi Gabriella , Isaia Gianluca 
Published: September 2011

AbstractAlzheimer's disease (AD) is the commonest form of dementia in the elderly, characterized by memory dysfunction, loss of lexical access, spatial and temporal disorientation, and impaired judgment. A growing body of scientific literature addresses the implication of dietary habits in the pathogenesis of AD. This review reports recent findings concerning the modulation of AD development by dietary lipids, in animals and humans, focusing on the pathogenetic role of lipid oxidation products. Oxidative breakdown products of ω‐6 polyunsaturated fatty acids (ω‐6 PUFAs), and cholesterol oxidation products (oxysterols), might play a role in favoring β‐amyloid deposition, a hallmark of AD's onset and progression. Conversely, ω‐3 PUFAs appear to contribute to preventing and treating AD. However, high concentrations of ω‐3 PUFAs can also produce oxidized derivatives reacting with important functions of nervous cells. Thus, altered balances between cholesterol and oxysterols, and between ω‐3 and ω‐6 PUFAs must be considered in AD's pathophysiology. The use of a diet with an appropriate ω‐3/ω‐6 PUFA ratio, rich in healthy oils, fish and antioxidants, such as flavonoids, but low in cholesterol‐containing foods, can be a beneficial component in the clinical strategies of prevention of AD.
An adaptive edge element method and its convergence for a Saddle‐Point problem from magnetostatics
Authors: Chen Junqing , Xu Yifeng , Zou Jun 
Published: September 2011

AbstractReliable and efficient a posteriori error estimates are derived for the edge element discretization of a saddle‐point Maxwell's system. By means of the error estimates, an adaptive edge element method is proposed and its convergence is rigorously demonstrated. The algorithm uses a marking strategy based only on the error indicators, without the commonly used information on local oscillations and the refinement to meet the standard interior node property. Some new ingredients in the analysis include a novel quasi‐orthogonality and a new inf‐sup inequality associated with an appropriately chosen norm. It is shown that the algorithm is a contraction for the sum of the energy error plus the error indicators after each refinement step. Numerical experiments are presented to show the robustness and effectiveness of the proposed adaptive algorithm. © 2011 Wiley Periodicals, Inc. Numer Methods Partial Differential Eq, 2012
Factors Associated With Parental Perception of Child Vulnerability 12 Months After Abnormal Newborn Screening Results
Authors: Tluczek Audrey , McKechnie Anne Chevalier , Brown Roger L. 
Published: September 2011

AbstractWe identified factors associated with elevated parental perceptions of child vulnerability (PPCV) 12 months after newborn screening (NBS) of 136 children: healthy, normal results (H, n = 37), cystic fibrosis carriers (CF‐C, n = 40), congenital hypothyroidism (CH, n = 36), and cystic fibrosis (CF, n = 23). Controlling for infant and parent characteristics, mixed logit structural equation modeling showed direct paths to elevated PPCV included parent female sex, CF diagnosis, and high documented illness frequency. PPCV was positively associated with maternal parenting stress. Infants with CF and CF carriers had significantly more documented illness frequency than H group infants. The CH group did not differ significantly from the H group and had no paths to PPCV. Unexpectedly high documented illness frequency among infants who are CF carriers warrants further investigation. © 2011 Wiley Periodicals, Inc. Res Nurs Health 34:389–400, 2011
Intracoronary delivery of DNAzymes targeting human EGR‐1 reduces infarct size following myocardial ischaemia reperfusion
Authors: Bhindi Ravinay , Fahmy Roger G , McMahon Aisling C , Khachigian Levon M , Lowe Harry C 
Published: September 2011

AbstractDespite improvements in treatment, myocardial infarction (MI) remains an important cause of morbidity and mortality. Inflammation arising from ischaemic and reperfusion injury is a key mechanism which underpins myocardial damage and impairment of cardiac function. Early growth response‐1 (Egr‐1) is an early immediate gene and a master regulator that has been implicated in the pathogenesis of ischaemia‐reperfusion (IR) injury. This study sought to examine the effect of selective inhibition of Egr‐1 using catalytic deoxyribonucleic acid molecules (DNAzymes, DZs) delivered via the clinically relevant coronary route in a large animal model of myocardial IR. It was hypothesized that Egr‐1 inhibition with intracoronary DZ would reduce infarction size by modulating its downstream effector molecules. Egr‐1 DZs inhibited the adherence of THP‐1 monocytes to IL‐1β‐activated endothelial cells in vitro and retained its catalytic activity up to 225 min after in vivo administration. In a porcine model of myocardial IR (45 min ischaemia/3 h reperfusion), DZ was taken up in the cytoplasm and nuclei of cardiomyocytes and endothelial cells in the myocardium after intracoronary delivery. Egr‐1 DZs reduced infarct size and improved cardiac functional recovery following intracoronary delivery at the initiation of IR in this large animal model of MI. This was associated with inhibition of pro‐inflammatory Egr‐1 and ICAM‐1 expression, and the reduced expression of TNF‐α, PAI‐1, TF, and myocardial MPO activity in tissue derived from the border zone of the infarct. Taken together, these data suggest that strategies targeting Egr‐1 via the intracoronary route after IR injury in pigs have potential therapeutic implications in human MI. Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Crosslink network evolution of nature rubber/zinc dimethacrylate composite during peroxide vulcanization
Authors: Chen Yukun , Xu Chuanhui 
Published: September 2011

AbstractIn this article, we investigated the evolution of the crosslink networks in nature rubber (NR) which is filled with in situ zinc dimethacrylate (ZDMA) during vulcanization. The results revealed that a primary network dominated by the ionic bond could be formed in the first 1 min, while the backbone of the covalent crosslink network was not formed. The ionic crosslinks, cooperating with some other physical crosslinks and some primary covalent crosslink points, play an important role in supporting the crosslink backbone of the composite in this period. This primary network was strong enough to bear the force exerted by the moving die of the Rheometer and to enable the composite that do not dissolve in toluene. After that, both the covalent crosslinking of NR molecules and polymerization of ZDMA react rapidly to give a birth to the fundamental network of the composites. Fourier transform infrared spectroscopy and differential scanning calorimeter were also used to investigate the curing reaction of the NR/ZDMA compounds. After ZDMA reaching a considerable conversion, the substantial covalent crosslinking reaction starts to be initiated. These results support the primary network dominated by the ionic bond formed in the first 1 min. POLYM. COMPOS., 2011. © 2011 Society of Plastics Engineers
Pattern of occurrence and occupancy of carbonylation sites in proteins
Authors: Rao R. Shyama Prasad , Møller Ian Max 
Published: September 2011

AbstractProteins are targets for modification by reactive oxygen species, and carbonylation is an important irreversible modification that increases during oxidative stress. While information on protein carbonylation is accumulating, its pattern is not yet understood. We have made a meta‐analysis of the available literature data (456 carbonylation sites on 208 proteins) to appreciate the nature of carbonylation sites in proteins. Of the carbonylated (Arg, Lys, Pro, and Thr – RKPT) amino acids, Lys is the most abundant, whereas Pro is the most susceptible and Thr is the least susceptible. The incidence of carbonylation is lower in the N‐terminal part of the protein primary sequence. Although a significantly higher number of carbonylated sites occur in Arg‐, Lys‐, Pro‐ and Thr‐rich regions of proteins, the hydropathy environment of carbonylated sites is not significantly different from potential carbonylation sites. Comparison of metal‐catalyzed oxidation of two closely related proteins indicates that this type of carbonylation might not be very specific in proteins. Interestingly, carbonylated sites show a very strong tendency to cluster together in the protein primary sequence hinting at some sort of discerning mechanism. While some attributes of protein carbonylation appear to be random, further investigations are warranted to appreciate the deterministic nature of protein carbonylation sites.
Virus‐induced dilated cardiomyopathy is characterized by increased levels of fibrotic extracellular matrix proteins and reduced amounts of energy‐producing enzymes
Authors: Nishtala Krishnatej , Phong Truong Q. , Steil Leif , Sauter Martina , Salazar Manuela G. , Kandolf Reinhard , Kroemer Heyo K. , Felix Stephan B. , Völker Uwe , Klingel Karin , Hammer Elke 
Published: September 2011

AbstractThe most relevant clinical phenotype resulting from chronic enteroviral myocarditis is dilated cardiomyopathy (DCM). Mice of the susceptible mouse strain A.BY/SnJ mimick well human DCM since they develop as a consequence of persistent infection and chronic inflammation a dilation of the heart ventricle several weeks after coxsackievirus B3 (CVB3) infection. Therefore, this model is well suited for the analysis of changes in the heart proteome associated with DCM. Here, we present a proteomic survey of the dilated hearts based on differential fluorescence gel electrophoresis and liquid chromatography–mass spectrometric centered methods in comparison to age‐matched non‐infected hearts. In total, 101 distinct proteins, which belong to categories immunity and defense, cell structure and associated proteins, energy metabolism and protein metabolism/modification differed in their levels in both groups. Levels of proteins involved in fatty acid metabolism and electron transport chain were found to be significantly reduced in infected mice suggesting a decrease in energy production in CVB3‐induced DCM. Furthermore, proteins associated with muscle contraction (MLRV, MLRc2, MYH6, MyBPC3), were present in significantly altered amounts in infected mice. A significant increase in the level of extracellular matrix proteins in the dilated hearts indicates cardiac remodeling due to fibrosis.
Luminescence and scintillation of Ce3+‐doped oxide glass with high Gd2O3 concentration
Authors: Chewpraditkul W. , He X. , Chen D. , Shen Y. , Sheng Q. , Yu B. , Nikl M. , Kucerkova R. , Beitlerova A. , Wanarak C. , Phunpueok A. 
Published: September 2011

AbstractCe3+‐doped oxide glass with high Gd2O3 (30 mol%) concentration was prepared as a scintillating glass under CO reducing atmosphere. A dominant emission band of the Ce3+ 5d → 4f transition peaking around 400 nm was observed in the photo‐ and radioluminescence (PL, RL) spectra. PL decay was governed by a few tens of nanoseconds decay time. The integral scintillation efficiency of about 30% of the Bi4Ge3O12 scintillator was observed as well. It exhibits a light yield of about 910 photons/MeV with an energy resolution of 18.3% (FWHM) in the pulse height spectrum of 662 keV γ‐rays from a 137Cs source.
Software abstractions for mobile RFID‐enabled applications
Authors: Carreton Andoni Lombide , Pinte Kevin , Meuter Wolfgang De 
Published: September 2011

SUMMARYOur everyday environments may soon be pervaded with radio frequency identification (RFID) tags integrated in physical objects. These RFID tags can store a digital representation of the physical object and transmit it wirelessly to pervasive, context‐aware applications running on mobile devices. However, communicating with RFID tags is prone to many failures inherent to the technology. This hinders the development of such applications, as traditional programming models require the programmer to deal with the RFID hardware characteristics manually. On the other hand, traditional RFID middleware focuses on limited scenarios in an enterprise context and not on general ubiquitous computing scenarios. In this paper, we extend the ambient‐oriented programming paradigm to program RFID applications, by considering RFID tags as intermittently connected mutable proxy objects hosted on mobile distributed computing devices, and detail our prototype implementation. Copyright © 2011 John Wiley & Sons, Ltd.
School expulsion as a process and an event: Before and after effects on children at risk for school discipline
Authors: Morrison Gale M. , Anthony Suzanne , Storino Meri H. , Cheng Joanna J. , Furlong Michael J. , Morrison Richard L. 
Published: September 2011

AbstractA better understanding of expulsion as an act, a process, and an educational intervention leads to an entirely different understanding of student behavior and disciplinary consequences.