bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lysozyme M–Positive Monocytes Mediate Angiotensin II–Induced Arterial Hypertension and Vascular Dysfunction
Authors: Wenzel Philip , Knorr Maike , Kossmann Sabine , Stratmann Jan , Hausding Michael , Schuhmacher Swenja , Karbach Susanne H. , Schwenk Melanie , Yogev Nir , Schulz Eberhard , Oelze Matthias , Grabbe Stephan , Jonuleit Helmut , Becker Christian , Daiber Andreas , Waisman Ari , Münzel Thomas 
Published: August 2011

Background—
Angiotensin II (ATII), a potent vasoconstrictor, causes hypertension, promotes infiltration of myelomonocytic cells into the vessel wall, and stimulates both vascular and inflammatory cell NADPH oxidases. The predominant source of reactive oxygen species, eg, vascular (endothelial, smooth muscle, adventitial) versus phagocytic NADPH oxidase, and the role of myelomonocytic cells in mediating arterial hypertension have not been defined yet.

Methods and Results—

Angiotensin II (1 mg · kg
−1
· d
−1
for 7 days) increased the number of both CD11b
+
Gr-1
low
F4/80
+
macrophages and CD11b
+
Gr-1
high
F4/80
−
neutrophils in mouse aorta (verified by flow cytometry). Selective ablation of lysozyme M-positive (LysM
+
) myelomonocytic cells by low-dose diphtheria toxin in mice with inducible expression of the diphtheria toxin receptor (LysM
iDTR
mice) reduced the number of monocytes in the circulation and limited ATII-induced infiltration of these cells into the vascular wall, whereas the number of neutrophils was not reduced. Depletion of LysM
+
cells attenuated ATII-induced blood pressure increase (measured by radiotelemetry) and vascular endothelial and smooth muscle dysfunction (assessed by aortic ring relaxation studies) and reduced vascular superoxide formation (measured by chemiluminescence, cytochrome c assay, and oxidative fluorescence microtopography) and the expression of NADPH oxidase subunits gp91
phox
and p67
phox
(assessed by Western blot and mRNA reverse-transcription polymerase chain reaction). Adoptive transfer of wild-type CD11b
+
Gr-1
+
monocytes into depleted LysM
iDTR
mice reestablished ATII-induced vascular dysfunction, oxidative stress, and arterial hypertension, whereas transfer of CD11b
+
Gr-1
+
neutrophils or monocytes from gp91
phox
or ATII receptor type 1 knockout mice did not.

Conclusions—
Infiltrating monocytes with a proinflammatory phenotype and macrophages rather than neutrophils appear to be essential for ATII-induced vascular dysfunction and arterial hypertension.
Developing High-Quality Field Program Sounding Datasets
Authors: Ciesielski Paul E. , Haertel Patrick T. , Johnson Richard H. , Wang Junhong , Loehrer Scot M. 
Published: August 2011

Enormous resources of time, effort, and finances are expended in collecting field program rawinsonde (sonde) datasets. Correcting the data and performing quality control (QC) in a timely fashion after the field phase of an experiment are important for facilitating scientific research while interest is still high and funding is available. However, a variety of issues (different sonde types, ground station software, data formats, quality control issues, sonde errors, etc.) often makes working with these datasets difficult and time consuming.
Our experience working with sounding data for several field programs has led to the design of a general procedure for creating user-friendly, bias-reduced, QCed sonde datasets. This paper describes the steps in this procedure, gives examples for the various processing stages, and provides access to software tools to aide in this process.
Depressive symptoms in first-and second-generation migrants: A cross-sectional study of a multi-ethnic working population
Authors: Sieberer Marcel , Maksimović Saša , Ersöz Burcu , Machleidt Wielant , Ziegenbein Marc , Calliess Iris T. 
Published: August 2011

Background: Migrants in Europe may suffer from depression more often than the native-born population of the particular host country. Reports about the prevalence of depression in migrants are, however, heterogeneous and the possible causes are the subject of controversial discussion. Aims: The aims of this study are to determine the incidence of depressiveness in a large multi-ethnic working population with and without a history of migration, and to investigate possible connections with migration status and acculturation criteria. Methods: The cross-sectional study asked 7062 employees of a university hospital to complete a self-rating questionnaire concerning socio-demographic data, migration status and indicators of acculturation. Depressiveness was assessed by means of the German version of the Center of Epidemiological Studies Depression Scale (CES-D). Results: The response rate was 41.7% ( N = 2932); 14.9% of the participants ( n = 419) reported a history of migration, 275 (65.8%) of whom were first-generation (M1) and 143 (34.2%) second-generation (M2) migrants. According to the CES-D scores, 8.7% of non-migrants ( n = 207) suffered from clinically relevant depressive symptoms, compared to 16% ( n = 44) of the M1 group (OR = 2.10, 95% CI: 1.44–3.04, p < .001) and 14% ( n = 20) in M2 (OR = 1.68, 95% CI: 1.01–2.79, p = .048). Taking gender into consideration revealed that only the female migrants showed a statistically significant increased rate of depressiveness (χ 2 = 16.68, p < .001). Conclusions: Our results suggest that first- and second-generation female migrants are more likely to suffer from depressiveness than non-migrant females. In this model a history of migration is shown to be an independent risk factor for depressiveness.
Head and Neck Rhabdomyosarcoma
Authors: Turner Justin H. , Richmon Jeremy D. 
Published: August 2011

ObjectiveTo evaluate trends in incidence, survival, and treatment of rhabdomyosarcoma (RMS) of the head and neck.Study DesignRetrospective review of a national database.SettingTertiary medical center.Subject and MethodsIncidence and survival trends were examined for head and neck RMS diagnosed between 1973 and 2007 using the Surveillance, Epidemiology, and End Results Program. Frequencies, incidence rates, and relative survival curves were calculated for various RMS subtypes and primary sites.ResultsBetween 1973 and 2007, the incidence of RMS of the head and neck increased significantly, with an annual percentage change of 1.16%. Relative 5‐year survival was statistically unchanged during the study period at 62.8% ± 2.3%. When analyzed by univariate analysis, overall survival was found to be dependent on sex, age, primary site, extent of disease, and histology. When evaluated by stage, most orbital tumors (60.6%) presented with localized disease, while most parameningeal tumors presented with either regional (53.2%) or distant (28.1%) spread. Multivariate analysis found that age less than 10 years at diagnosis and tumors with localized or regional spread were associated with improved overall survival. Relative survival was found to be largely dependent on extent of disease rather than primary site.ConclusionsDespite reported advances in overall and disease‐free survival for patients with RMS, population‐based analysis shows no substantial improvement during the past 30+ years. The prognosis of these patients is largely dependent on extent of disease at diagnosis.
Concentration of Hyaluronic Acid in Human Vocal Folds in Young and Old Subjects
Authors: Korn Gustavo Polacow , Martins João Roberto Maciel , Park Sung Woo , Mendes Aline , Kobayashi Elsa Yoko , Nader Helena Bonciani , De Biase Noemi Grigoletto 
Published: August 2011

ObjectiveTo compare and evaluate concentrations of hyaluronic acid (HA) in normal vocal folds of both young and elderly people of both sexes.Study DesignExperimental study.SettingThe research was carried out at the ENT, Head and Neck Surgery Department and the Pharmacology Institute of São Paulo Federal University.Subjects and MethodsEnzyme‐linked immunosorbent assay plates coated with biotin‐conjugated binding protein and streptavidin‐europium conjugate were sequentially incubated with samples from the middle portion of the cover, vocal ligament, and vocal muscle of 19 young and 20 elderly subjects of both sexes. After the release of europium in an enhancement solution, final fluorescence was measured using a fluorometer.ResultsStatistically significant comparisons: (1) all subject groups combined exhibited a higher HA concentration in the cover and ligament compared with the muscle samples; (2) women subjects had a significantly lower HA concentration in muscle compared with the ligament, and male subjects showed significantly lower HA in muscle compared with the cover; and (3) both genders of young subjects showed a higher HA concentration in ligament samples compared with the muscle.ConclusionHA concentrations are higher in the cover and ligament compared with the muscle in both genders and age groups, and there is a higher HA concentration in ligament compared with the muscle of young subjects of both genders. HA levels in the cover samples of younger women showed great variability that may relate to ovarian hormone levels, reflecting different phases of the menstrual cycle.
Intratympanic Steroids for Sudden Sensorineural Hearing Loss
Authors: Spear Samuel A. , Schwartz Seth R. 
Published: August 2011

ObjectiveTo determine the evidence for efficacy of intratympanic (IT) steroid treatment for the management of idiopathic sudden sensorineural hearing loss through a systematic review of the existing literature.Data SourcesOnline databases consisting of PubMed, Medline, OVID, Google Scholar, Cochrane Review, and collected additional publications cited in bibliographies.Review MethodsLiterature search by both authors with structured criteria to select studies evaluated for systematic review.ResultsThe authors identified 176 articles, of which 32 represented studies of initial or salvage intratympanic steroid injections for sudden hearing loss that included 6 randomized trials and only 2 randomized controlled trials. Despite the paucity of well‐executed trials, the vast majority of studies of intratympanic steroids for salvage treatment in all tiers demonstrated a benefit of intratympanic steroid therapy. A limited meta‐analysis of the higher quality studies revealed a mean difference in improvement of 13.3 dB (95% confidence interval [CI], 7.7‐18.9; P <. 0001). Although statistically significant, it is still unclear if this difference is clinically significant. Initial intratympanic therapy was equivalent to standard therapy in the existing literature of all qualities.ConclusionIntratympanic steroid treatment as primary treatment for sudden sensorineural hearing loss appears equivalent to treatment with high‐dose oral prednisone therapy. As salvage therapy, intratympanic steroids offer the potential for some degree of additional hearing recovery, although it remains uncertain if this improvement is clinically significant and what percentage of patients is likely to show benefit.
Effect of Adenotonsillectomy on Endothelin‐1 and C‐Reactive Protein Levels in Children with Sleep‐Disordered Breathing
Authors: Tatlıpınar Arzu , Çimen Burak , Duman Dursun , Esen Erkan , Köksal Sema , Gökçeer Tanju 
Published: August 2011

ObjectiveThis study aimed to evaluate the influence of adenotonsillectomy on the plasma concentration of endothelin‐1 (ET‐1) and C‐reactive protein (CRP) in children with sleep‐disordered breathing (SDB). The relationship between quality of life and ET‐1 levels was also evaluated.SettingTertiary referral center.Study DesignBefore‐and‐after case series.MethodsFasting blood samples for ET‐1 and high‐sensitivity CRP were drawn preoperatively in all patients and at 3 to 4 months postoperatively. The Obstructive Sleep Apnea–18 (OSA‐18) survey and Brouilette symptom score were completed by each child’s parents during the same time periods.ResultsThe mean ET‐1 level decreased from 3.51 ± 0.93 fmol/mL to 2.67 ± 0.69 fmol/mL postoperatively (P <. 01). OSA‐18 survey scores and Brouilette symptom scores also decreased in the postoperative period (P <. 01). When comparing moderate and severe cases to mild cases according to Brouilette scores, ET‐1 levels were significantly higher in moderate and severe cases (P <. 01). There was a significant correlation between ET‐1 and the OSA‐18 survey scale (r = 0.442; P =. 001). Although CRP levels decreased from 0.63 ± 1.19 mg/dL to 0.31 ± 0.23 mg/dL postoperatively, this was not statistically significant.ConclusionAdenotonsillectomy effectively lowered plasma ET‐1 levels in children with SDB and thus may have reduced their related risk for cardiovascular disease. In addition, adenotonsillectomy improved quality of life in this group.
The BCL11B tumor suppressor is mutated across the major molecular subtypes of T-cell acute lymphoblastic leukemia
Authors: Gutierrez Alejandro , Kentsis Alex , Sanda Takaomi , Holmfeldt Linda , Chen Shann-Ching , Zhang Jianhua , Protopopov Alexei , Chin Lynda , Dahlberg Suzanne E. , Neuberg Donna S. , Silverman Lewis B. , Winter Stuart S. , Hunger Stephen P. , Sallan Stephen E. , Zha Shan , Alt Frederick W. , Downing James R. , Mullighan Charles G. , Look A. Thomas 
Published: August 2011

Abstract
The BCL11B transcription factor is required for normal T-cell development, and has recently been implicated in the pathogenesis of T-cell acute lymphoblastic leukemia (T-ALL) induced by TLX overexpression or Atm deficiency. To comprehensively assess the contribution of BCL11B inactivation to human T-ALL, we performed DNA copy number and sequencing analyses of T-ALL diagnostic specimens, revealing monoallelic BCL11B deletions or missense mutations in 9% (n = 10 of 117) of cases. Structural homology modeling revealed that several of the BCL11B mutations disrupted the structure of zinc finger domains required for this transcription factor to bind DNA. BCL11B haploinsufficiency occurred across each of the major molecular subtypes of T-ALL, including early T-cell precursor, HOXA-positive, LEF1-inactivated, and TAL1-positive subtypes, which have differentiation arrest at diverse stages of thymocyte development. Our findings provide compelling evidence that BCL11B is a haploinsufficient tumor suppressor that collaborates with all major T-ALL oncogenic lesions in human thymocyte transformation.
The impact of modern migrations on present-day multi-ethnic Argentina as recorded on the mitochondrial DNA genome
Authors: Catelli María Laura , Álvarez-Iglesias Vanesa , Gómez-Carballa Alberto , Mosquera-Miguel Ana , Romanini Carola , Borosky Alicia , Amigo Jorge , Carracedo Ángel , Vullo Carlos , Salas Antonio 
Published: August 2011

Abstract

Background
The genetic background of Argentineans is a mosaic of different continental ancestries. From colonial to present times, the genetic contribution of Europeans and sub-Saharan Africans has superposed to or replaced the indigenous genetic 'stratum'. A sample of 384 individuals representing different Argentinean provinces was collected and genotyped for the first and the second mitochondrial DNA (mtDNA) hypervariable regions, and selectively genotyped for mtDNA SNPs. This data was analyzed together with additional 440 profiles from rural and urban populations plus 304 from Native American Argentineans, all available from the literature. A worldwide database was used for phylogeographic inferences, inter-population comparisons, and admixture analysis. Samples identified as belonging to hg (hg) H2a5 were sequenced for the entire mtDNA genome.

Results
Phylogenetic and admixture analyses indicate that only half of the Native American component in urban Argentineans might be attributed to the legacy of extinct ancestral Argentineans and that the Spanish genetic contribution is slightly higher than the Italian one. Entire H2a5 genomes linked these Argentinean mtDNAs to the Basque Country and improved the phylogeny of this Basque autochthonous clade. The fingerprint of African slaves in urban Argentinean mtDNAs was low and it can be phylogeographically attributed predominantly to western African. The European component is significantly more prevalent in the Buenos Aires province, the main gate of entrance for Atlantic immigration to Argentina, while the Native American component is larger in North and South Argentina. AMOVA, Principal Component Analysis and hgs/haplotype patterns in Argentina revealed an important level of genetic sub-structure in the country.

Conclusions
Studies aimed to compare mtDNA frequency profiles from different Argentinean geographical regions (e.g., forensic and case-control studies) should take into account the important genetic heterogeneity of the country in order to prevent false positive claims of association in disease studies or inadequate evaluation of forensic evidence.
Involvement of Cox-2 in the metastatic potential of chemotherapy-resistant breast cancer cells
Authors: Kang Ju-Hee , Song Ki-Hoon , Jeong Kyung-Chae , Kim Sunshin , Choi Changsun , Lee Chang Hoon , Oh Seung Hyun 
Published: August 2011

Abstract

Background
A major problem with the use of current chemotherapy regimens for several cancers, including breast cancer, is development of intrinsic or acquired drug resistance, which results in disease recurrence and metastasis. However, the mechanisms underlying this drug resistance are unknown. To study the molecular mechanisms underlying the invasive and metastatic activities of drug-resistant cancer cells, we generated a doxorubicin-resistant MCF-7 breast cancer cell line (MCF-7/DOX).

Methods
We used MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assays, flow cytometry assays, DNA fragmentation assays, Western blot analysis, cell invasion assays, small interfering RNA (siRNA) transfection, reverse transcription-polymerase chain reaction, experimental lung metastasis models, and gelatin and fibrinogen/plasminogen zymography to study the molecular mechanism of metastatic activities in MCF-7/DOX cells.

Results
We found that MCF-7/DOX acquired invasive activities. In addition, Western blot analysis showed increased expression of epidermal growth factor receptor (EGFR) and Cox-2 in MCF-7/DOX cells. Inhibition of Cox-2, phosphoinositide 3-kinase (PI3K)/Akt, or mitogen-activated protein kinase (MAPK) pathways effectively inhibited the invasive activities of MCF-7/DOX cells. Gelatin and fibrinogen/plasminogen zymography analysis showed that the enzymatic activities of matrix metalloproteinase-2 (MMP-2), MMP-9, and urokinase-type plasminogen activator were markedly higher in MCF-7/DOX cells than in the MCF-7 cells. In vitro invasion assays and mouse models of lung metastasis demonstrated that MCF-7/DOX cells acquired invasive abilities. Using siRNAs and agonists specific for prostaglandin E (EP) receptors, we found that EP1 and EP3 played important roles in the invasiveness of MCF-7/DOX cells.

Conclusions
We found that the invasive activity of MCF-7/DOX cells is mediated by Cox-2, which is induced by the EGFR-activated PI3K/Akt and MAPK pathways. In addition, EP1 and EP3 are important in the Cox-2-induced invasion of MCF-7/DOX cells. Therefore, not only Cox-2 but also EP1 and EP3 could be important targets for chemosensitization and inhibition of metastasis in breast cancers that are resistant to chemotherapy.
HPV vaccine decision making in pediatric primary care: a semi-structured interview study
Authors: Hughes Cayce C , Jones Amanda L , Feemster Kristen A , Fiks Alexander G 
Published: August 2011

AbstractBackgroundDespite national recommendations, as of 2009 human papillomavirus (HPV) vaccination rates were low with < 30% of adolescent girls fully vaccinated. Research on barriers to vaccination has focused separately on parents, adolescents, or clinicians and not on the decision making process among all participants at the point of care. By incorporating three distinct perspectives, we sought to generate hypotheses to inform interventions to increase vaccine receipt.MethodsBetween March and June, 2010, we conducted qualitative interviews with 20 adolescent-mother-clinician triads (60 individual interviews) directly after a preventive visit with the initial HPV vaccine due. Interviews followed a guide based on published HPV literature, involved 9 practices, and continued until saturation of the primary themes was achieved. Purposive sampling balanced adolescent ages and practice type (urban resident teaching versus non-teaching). Using a modified grounded theory approach, we analyzed data with NVivo8 software both within and across triads to generate primary themes.ResultsThe study population was comprised of 20 mothers (12 Black, 9 < high school diploma), 20 adolescents (ten 11-12 years old), and 20 clinicians (16 female). Nine adolescents received the HPV vaccine at the visit, eight of whom were African American. Among the 11 not vaccinated, all either concurrently received or were already up-to-date on Tdap and MCV4. We did not observe systematic patterns of vaccine acceptance or refusal based on adolescent age or years of clinician experience. We identified 3 themes: (1) Parents delayed, rather than refused vaccination, and when they expressed reluctance, clinicians were hesitant to engage them in discussion. (2) Clinicians used one of two strategies to present the HPV vaccine, either presenting it as a routine vaccine with no additional information or presenting it as optional and highlighting risks and benefits. (3) Teens considered themselves passive participants in decision making, even when parents and clinicians reported including them in the process.ConclusionsPrograms to improve HPV vaccine delivery in primary care should focus on promoting effective parent-clinician communication. Research is needed to evaluate strategies to help clinicians engage reluctant parents and passive teens in discussion and measure the impact of distinct clinician decision making approaches on HPV vaccine delivery.
Investigation of the adaptation of Lactococcus lactis to isoleucine starvation integrating dynamic transcriptome and proteome information
Authors: Dressaire Clémentine , Redon Emma , Gitton Christophe , Loubière Pascal , Monnet Véronique , Cocaign-Bousquet Muriel 
Published: August 2011

Abstract

Background
Amino acid assimilation is crucial for bacteria and this is particularly true for Lactic Acid Bacteria (LAB) that are generally auxotroph for amino acids. The global response of the LAB model Lactococcus lactis ssp. lactis was characterized during progressive isoleucine starvation in batch culture using a chemically defined medium in which isoleucine concentration was fixed so as to become the sole limiting nutriment. Dynamic analyses were performed using transcriptomic and proteomic approaches and the results were analysed conjointly with fermentation kinetic data.

Results
The response was first deduced from transcriptomic analysis and corroborated by proteomic results. It occurred progressively and could be divided into three major mechanisms: (i) a global down-regulation of processes linked to bacterial growth and catabolism (transcription, translation, carbon metabolism and transport, pyrimidine and fatty acid metabolism), (ii) a specific positive response related to the limiting nutrient (activation of pathways of carbon or nitrogen metabolism and leading to isoleucine supply) and (iii) an unexpected oxidative stress response (positive regulation of aerobic metabolism, electron transport, thioredoxin metabolism and pyruvate dehydrogenase). The involvement of various regulatory mechanisms during this adaptation was analysed on the basis of transcriptomic data comparisons. The global regulator CodY seemed specifically dedicated to the regulation of isoleucine supply. Other regulations were massively related to growth rate and stringent response.

Conclusion
This integrative biology approach provided an overview of the metabolic pathways involved during isoleucine starvation and their regulations. It has extended significantly the physiological understanding of the metabolism of L. lactis ssp. lactis. The approach can be generalised to other conditions and will contribute significantly to the identification of the biological processes involved in complex regulatory networks of micro-organisms.