bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimization of a highly standardized carboxyfluorescein succinimidyl ester flow cytometry panel and gating strategy design using discriminative information measure evaluation
Authors: Chan Cliburn , Lin Lin , Frelinger Jacob , Hérbert Valérie , Gagnon Dominic , Landry Claire , Sékaly Rafick‐Pierre , Enzor Jennifer , Staats Janet , Weinhold Kent J. , Jaimes Maria , West Mike 
Published: November 2010

AbstractThe design of a panel to identify target cell subsets in flow cytometry can be difficult when specific markers unique to each cell subset do not exist, and a combination of parameters must be used to identify target cells of interest and exclude irrelevant events. Thus, the ability to objectively measure the contribution of a parameter or group of parameters toward target cell identification independent of any gating strategy could be very helpful for both panel design and gating strategy design. In this article, we propose a discriminative information measure evaluation (DIME) based on statistical mixture modeling; DIME is a numerical measure of the contribution of different parameters towards discriminating a target cell subset from all the others derived from the fitted posterior distribution of a Gaussian mixture model. Informally, DIME measures the “usefulness” of each parameter for identifying a target cell subset. We show how DIME provides an objective basis for inclusion or exclusion of specific parameters in a panel, and how ranked sets of such parameters can be used to optimize gating strategies. An illustrative example of the application of DIME to streamline the gating strategy for a highly standardized carboxyfluorescein succinimidyl ester (CFSE) assay is described. © 2010 International Society for Advancement of Cytometry
Accuracy, latency, and energy cross‐optimization in wireless sensor networks through infection spreading
Authors: Bai Fan , Munasinghe Kumudu S. , Jamalipour Abbas 
Published: November 2010

AbstractIn this paper, cross‐optimization of accuracy, latency, and energy in wireless sensor networks (WSNs) through infection spreading is investigated. Our solution is based on a dual‐layer architecture for efficient data harvesting in a WSN, in which, the lower layer sensors are equipped with a novel adaptive data propagation method inspired by infection spreading and the upper layer consists of randomly roaming data harvesting agents. The proposed infection spreading mechanisms, namely random infection (RI) and linear infection (LI), are implemented at the lower layer. The entire sensor field is dynamically separated into several busy areas (BA) and quiet areas (QA). According to the BA or QA classification, the level of importance is defined, on which, the optimal number of infections for a particular observation is evaluated. Therefore, the accessed probability for observations with a relatively higher importance level is adaptively increased. The proposed mechanisms add further value to the data harvesting operation by compensating for its potential lack of coverage due to random mobility and tolerable delay, thus a relatively higher accuracy and latency requirements can be guaranteed for the optimization of energy consumption in a dynamically changing environment. Further, with the cost of processing simple location information, LI is proved to outperform RI. Copyright © 2010 John Wiley & Sons, Ltd.
Supramolecular and Intramolecular Energy Transfer with Ruthenium–Anthracene Donor–Acceptor Couples: Salt Bridge versus Covalent Bond
Authors: Freys Jonathan C. , Wenger Oliver S. 
Published: November 2010

AbstractThe formation of hydrogen‐bonded cation–anion adducts between the complex [Ru(bpy)2(biimH2)]2+ (bpy = 2,2′‐bipyridine; biimH2 = 2,2′‐biimidazole) and anthracene‐9‐carboxylate in dichloromethane solution was investigated by 1H NMR, optical absorption, and luminescence spectroscopy. The experimental data indicates that more than one anthracene‐9‐carboxylate anion can interact closely with the dicationic ruthenium complex. Energy transfer from the photoexcited ruthenium complex to anthracene‐9‐carboxylate in 1:1 adducts of these two components was investigated by transient absorption spectroscopy and compared to intramolecular energy transfer in a covalently linked ruthenium‐xylene‐anthracene dyad with a comparable donor–acceptor distance.
Facile Synthesis of Leaf‐Like CuO Nanoparticles and Their Application on Glucose Biosensor
Authors: Li Ying , Wei Yinyin , Shi Guoyue , Xian Yuezhong , Jin Litong 
Published: November 2010

AbstractAn efficient amperometric biosensor based on well‐crystallized leaf‐like CuO nanoparticles for detecting glucose has been proposed. The leaf‐like CuO nanoparticles, synthesized by a simple one‐step hydrothermal method, were characterized by X‐ray diffraction (XRD), scanning electron microscope (SEM) and transmission electron microscopy (TEM) for the morphology study. Under the optimal condition, the electrochemical behaviour of the leaf‐like CuO nanoparticles modified electrode for detection of glucose exhibited high sensitivity of 246 µA/mM/cm2, short response time (within 5 s), linear dynamic range from 1.0 to 170 µM (R2=0.9995), and low limit of detection (LOD) (S/N=3) of 0.91 µM. The high sensitivity, good reproducibility, stability, and fast amperometric sensing towards oxidation of glucose, make this biosensor promising for future application.
Nonenzymatic Electrochemical Glucose Sensor Based on Novel Copper Film
Authors: Sun Fang , Li Li , Liu Peng , Lian Yongfu 
Published: November 2010

AbstractNovel copper (Cu) film composed of pillar‐like structure was synthesized on indium‐doped tin oxide (ITO) substrate by electrodeposition in acetate bath with proline as additive for the first time and used to construct nonenzymatic glucose sensor. When applied to detect glucose, such prepared electrode showed low operating potential (0.4 V), high sensitivity (699.4499 µA mM−1 cm−2), and fast response time (<3 s) compared with other Cu‐based electrodes. In addition, the prepared electrode also offered good anti‐interference ability to ascorbic acid, uric acid and acetaminophen. Present study provides new insights into the control of Cu film morphology for sensor fabrication.
Selective Simultaneous Determination of Paracetamol and Uric Acid Using a Glassy Carbon Electrode Modified with Multiwalled Carbon Nanotube/Chitosan Composite
Authors: Babaei Ali , Garrett David J. , Downard Alison J. 
Published: November 2010

AbstractA multiwalled carbon nanotube/chitosan modified glassy carbon electrode (MWCNTs‐CHT/GCE) has been used for simultaneous determination of paracetamol (PAR) and uric acid (UA). The measurements were carried out using differential pulse voltammetry (DPV), cyclic voltammetry (CV) and chronoamperometry (CA). DPV measurements showed a linear relationship between oxidation peak current and concentration of PAR and UA in phosphate buffer (pH 7) over the concentration range 2 µM to 250 µM, and 10 µM to 400 µM, respectively. The analytical performance of this sensor has been evaluated for detection of PAR and UA in human serum and human urine with satisfactory results.
Determination of Lead and Cadmium in Sea‐ and Freshwater by Anodic Stripping Voltammetry with a Vibrating Bismuth Electrode
Authors: Bi Zhaoshun , Chapman Conrad S. , Salaün Pascal , van den Berg Constant M. G. 
Published: November 2010

AbstractA solid, bismuth (Bi), disk, electrode is used to determine lead (Pb) in natural waters including seawater. The diffusion layer thickness was lowered from 93 to 29 µm by stirring, and to 18 µm by using the vibrated version of the Bi electrode. The Bi electrode does not require removal of dissolved oxygen, which facilitates in situ detection. The electrode was tested for the determination of Pb in coastal seawater samples. The detection limit for Pb was 0.15 nM in acetate buffer and 0.5 nM in seawater using a 2 min deposition time. Cadmium can be determined together with Pb but the sensitivity is about 10×lower. The Bi electrode compares unfavourably to a mercury electrode in terms of sensitivity.
Head and neck paragangliomas
Authors: Mendenhall William M. , Amdur Robert J. , Vaysberg Mikhail , Mendenhall Charles M. , Werning John W. 
Published: November 2010

AbstractBackgroundThe purpose of this study was to describe the natural history and optimal treatment for head and neck paragangliomas (PGs).MethodsOur methods were the review of the pertinent literature.ResultsPGs are rare tumors seen most commonly in the head and neck. Approximately 90% are sporadic; the remainder are familial and related to mutations of the succinate dehydrogenase (SDH) gene complex. Most PGs are benign and slow growing; 6% to 19% are malignant, as evidenced by the development of metastases. PGs may be treated by complete resection or moderate‐dose radiotherapy with a ≥90% likelihood of cure. The optimal radiotherapy dose is approximately 45 Gy/25 fractions/5 weeks. The treatment modality selected depends on the risk of complications. Due to their rarity, the optimal treatment for malignant PGs is unclear.ConclusionPGs may be treated by either complete resection or radiotherapy with a high likelihood of success. Treatment depends on the location and extent of the PG and the morbidity associated with treatment. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Therapeutic effects of a fusogenic newcastle disease virus in treating head and neck cancer
Authors: Li Pingdong , Chen Chun‐Hao , Li Sen , Givi Babak , Yu Zhenkun , Zamarin Dmitriy , Palese Peter , Fong Yuman , Wong Richard J. 
Published: November 2010

AbstractBackgroundNewcastle disease virus (NDV) is a paramyxovirus that is pathogenic in birds but causes only mild flulike symptoms in human beings. NDV(F3aa)‐GFP is a genetically modified, fusogenic NDV. We assessed the utility of NDV(F3aa)‐GFP in treating head and neck squamous cell carcinoma.Methods and ResultsAt a multiplicity of infection (MOI) of 1, NDV(F3aa)‐GFP infection of 3 cell lines supported strong GFP expression by 36 hours. Four cell lines were highly sensitivite to viral cytotoxicity, with >75% of cells lysed by day 6 at MOI 0.1, and 2 other cell lines were partially susceptible. Murine SCC25 flank tumors exhibited robust GFP expression after a single intratumoral viral injection and showed near‐complete tumor regression over 34 days. There were no adverse effects attributable to therapy.ConclusionsWe demonstrate that a fusogenic NDV exerts potent oncolytic effects against human head and neck cancer and support its continued investigation for clinical application. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Hydrograph separation and precipitation source identification using stable water isotopes and conductivity: River Ganga at Himalayan foothills
Authors: Maurya A. S. , Shah Miral , Deshpande R. D. , Bhardwaj R. M. , Prasad A. , Gupta S. K. 
Published: November 2010

AbstractThe observed retreat of several Himalayan glaciers and snow packs is a cause of concern for the huge population in southern Asia that is dependent on the glacial‐fed rivers emanating from Himalayas. There is considerable uncertainty about how cryospheric recession in the Himalayan region will respond to climate change, and how the water resource availability will be affected. As a first step towards quantifying the contribution of glacier‐melt water, hydrograph separation of River Ganga at Rishikesh into its constituent components, namely (i) surface runoff, (ii) glacial ice‐melt and (iii) groundwater discharge has been done in this paper. A three‐component mixing model has been employed using the values of δ18O and electrical conductivity (EC) of the river water, and its constituents, to estimate the time‐varying relative fraction of each component. The relative fraction of the surface runoff peaks (70–90%) during winter, due to the near‐zero contribution of glacial ice‐melt, essentially represents the melting of surface snow from the catchment. The contribution of glacial ice‐melt to the stream discharge peaks during summer and monsoon reaches a maximum value of ∼40% with an average of 32%. The fraction of groundwater discharge varies within a narrow range (15 ± 5%) throughout the year. On the basis of the variation in the d‐excess values of river water, it is also suggested that the snow‐melt and ice‐melt component has a significant fraction derived from winter precipitation with moisture source from mid‐latitude westerlies (also known as western disturbances). Copyright © 2010 John Wiley & Sons, Ltd.
Implications of climate change in the twenty‐first century for simulated magnitude and frequency of bed‐material transport in tributaries of the Saint‐Lawrence River
Authors: Verhaar Patrick M. , Biron Pascale M. , Ferguson Robert I. , Hoey Trevor B. 
Published: November 2010

AbstractMore frequent extreme flood events are likely to occur in many areas in the twenty‐first century due to climate change. The impacts of these changes on sediment transport are examined at the event scale using a 1D morphodynamic model (SEDROUT4‐M) for three tributaries of the Saint‐Lawrence River (Québec, Canada) using daily discharge series generated with a hydrological model (HSAMI) from three global climate models (GCMs). For all tributaries, larger flood events occur in all future scenarios, leading to increases in bed‐material transport rates, number of transport events and number of days in the year where sediment transport occurs. The effective and half‐load discharges increase under all GCM simulations. Differences in flood timing within the tributaries, with a shift of peak annual discharge from the spring towards the winter, compared to the hydrograph of the Saint‐Lawrence River, generate higher sediment transport rates because of increased water surface slope and stream power. Previous research had shown that channel erosion is expected under all GCMs' discharge scenarios. This study shows that, despite lower bed elevations, flood risk is likely to increase as a result of higher flood magnitude, even with falling base level in the Saint‐Lawrence River. Copyright © 2010 John Wiley & Sons, Ltd.
Assessment of psychopathology in 2‐ to 5‐year‐olds: Applying the Infant–Toddler Social Emotional Assessment
Authors: Visser Janne C. , Smeekens Sanny , Rommelse Nanda , Verkes Robbert J. , van der Gaag Rutger J. , Buitelaar Jan K. 
Published: November 2010

AbstractThe information provided by parents is indispensable for the early identification of psychopathology; for this reason, developmentally appropriate, reliable, and valid questionnaires are needed to gather their information. This study was designed to examine the utility of the Infant Toddler Social Emotional Assessment (ITSEA), a parent‐report questionnaire covering a wide range of behavior and emotional problems and competencies, in preschoolers referred for child psychiatric evaluation. The fathers and mothers of 85 children (23.2% girls; age 15–57 months) with autism spectrum, externalizing, or internalizing disorders completed the ITSEA, the Child Behavior Checklist (CBCL/2–3 and 4–18 versions), and the child domain of the Parenting Stress Index (PSI). The ITSEA showed good interrater reliability between parents, and validity was supported by significant correlations with corresponding CBCl and PSI domains. Evidence supporting the validity of the ITSEA for psychopathology was mixed across scales, with good diagnostic accuracy at the level of the broader psychiatric syndromes being achieved by the combination of subscales within the Externalizing, Internalizing, and Competence domains. The value of the ITSEA lies in the ability to systematically evaluate a wide range of problem behaviors and competencies. The ITSEA may be useful to create profiles of children's functioning in preschoolers referred for psychiatric assessment.
The Rac1 exchange factor Dock5 is essential for bone resorption by osteoclasts
Authors: Vives Virginie , Laurin Mélanie , Cres Gaelle , Larrousse Pauline , Morichaud Zakia , Noel Danièle , Côté Jean-François , Blangy Anne 
Published: November 2010

Abstract
Osteoporosis, which results from excessive bone resorption by osteoclasts, is the major cause of morbidity for elder people. Identification of clinically relevant regulators is needed to develop novel therapeutic strategies. Rho GTPases have essential functions in osteoclasts by regulating actin dynamics. This is of particular importance because actin cytoskeleton is essential to generate the sealing zone, an osteoclast-specific structure ultimately mediating bone resorption. Here we report that the atypical Rac1 exchange factor Dock5 is necessary for osteoclast function both in vitro and in vivo. We discovered that establishment of the sealing zone and consequently osteoclast resorbing activity in vitro require Dock5. Mechanistically, our results suggest that osteoclasts lacking Dock5 have impaired adhesion that can be explained by perturbed Rac1 and p130Cas activities. Consistent with these functional assays, we identified a novel small-molecule inhibitor of Dock5 capable of hindering osteoclast resorbing activity. To investigate the in vivo relevance of these findings, we studied Dock5–/– mice and found that they have increased trabecular bone mass with normal osteoclast numbers, confirming that Dock5 is essential for bone resorption but not for osteoclast differentiation. Taken together, our findings characterize Dock5 as a regulator of osteoclast function and as a potential novel target to develop antiosteoporotic treatments. © 2011 American Society for Bone and Mineral Research.
Selenium suppressed hydrogen peroxide‐induced vascular smooth muscle cells calcification through inhibiting oxidative stress and ERK activation
Authors: Liu Hongmei , Lu Qian , Huang Kaixun 
Published: November 2010

AbstractAtherosclerosis is frequently associated with vascular calcification. Increasing evidences underline that the essential micronutrient selenium may prevent atherosclerosis, but the role of selenium in vascular calcification remains unknown. In this study, we assessed the effect of sodium selenite (Na2SeO3) on H2O2‐enhanced vascular smooth muscle cells (VSMCs) calcification and examined the involvement of extracellular signal‐regulated kinase (ERK) signaling pathway. Hydrogen peroxide enhanced vascular calcification by inducing osteoblastic differentiation of VSMCs, as showed by up‐regulating the mRNA expression of type I collagen, osteocalcin, and Runx2, a key transcription factor for osteoblastic differentiation, increasing alkaline phosphatase activity, and calcium deposition. These effects of H2O2 were suppressed by pretreatment of the cells with selenite (0.1–1 µM) for 24 h. In addition, H2O2 activated the phosphorylation of ERK1/2 and inhibition of H2O2‐activated ERK signaling by MEK inhibitor PD98059 blocked the effect of H2O2 on osteoblastic differentiation of VSMCs. Furthermore, H2O2 induced oxidative stress in calcifying VSMCs, as evidenced by the increase of intracellular reactive oxygen species production and malondialdehyde level, and the decrease of total protein thiols content and the activity of antioxidant selenoenzyme glutathione peroxidases. Selenite pretreatment also attenuated H2O2‐induced oxidative stress and ERK activation. These results suggested that selenite suppressed H2O2‐enhanced osteoblastic differentiation and calcification of VSMCs through inhibiting oxidative stress and ERK activation, indicating a potential preventive role for selenium in vascular calcification. J. Cell. Biochem. 111: 1556–1564, 2010. © 2010 Wiley‐Liss, Inc.
ProlongedNrf1overexpression triggers adipocyte inflammation and insulin resistance
Authors: van Tienen Florence H. , Lindsey Patrick J. , van der Kallen Carla J. , Smeets Hubert J. 
Published: November 2010

AbstractAdipose tissue is currently being recognized as an important endocrine organ, carrying defects in a number of metabolic diseases. Mitochondria play a key role in normal adipose tissue function and mitochondrial alterations can result in pathology, like lipodystrophy or type 2 diabetes. AlthoughPgc1αis regarded as the main regulator of mitochondrial function, downstreamNrf1is the key regulator of mitochondrial biogenesis.Nrf1is also involved in a wide range of other processes, including proliferation, innate immune response, and apoptosis. To determine transcriptional targets ofNrf1, 3T3‐L1 preadipocytes were transfected with either pNrf1 or a control vector. Two days post‐confluence, 3T3‐L1 preadipocytes were allowed to differentiate. At day 8 of differentiation,Nrf1overexpressing cells had an increased mtDNA copy number and reduced lipid content. This was not associated with an increased ATP production rate per cell. Using global gene expression analysis, we observed thatNrf1overexpression stimulated cell proliferation, apoptosis, and cytokine expression. In addition, prolongedNrf1induced an adipokine expression profile of insulin resistant adipocytes.Nrf1has a wide range of transcriptional targets, stimulators as well as inhibitors of adipose tissue functioning. Therefore, post‐transcriptional regulation ofNrf1, or stimulating specificNrf1targets may be a more suitable approach for stimulating mitochondrial biogenesis and treating adipose tissue defects, instead of directly stimulatingNrf1expression. In addition, our results show that short‐term effects can drastically differ from long‐term effects. J. Cell. Biochem. 111: 1575–1585, 2010. © 2010 Wiley‐Liss, Inc.
Mechanical integrin stress and magnetic forces induce biological responses in mesenchymal stem cells which depend on environmental factors
Authors: Kasten Annika , Müller Petra , Bulnheim Ulrike , Groll Jürgen , Bruellhoff Kristina , Beck Ulrich , Steinhoff Gustav , Möller Martin , Rychly Joachim 
Published: November 2010

AbstractThe control of mesenchymal stem cells (MSC) by physical cues is of great interest in regenerative medicine. Because integrin receptors function as mechanotransducers, we applied drag forces to β1 integrins on the apical surface of adherent human MSC. In addition to mechanical forces, the technique we used involved also the exposure of the cells to an inhomogeneous magnetic field. In order to assess the influence of the substrate on cell adhesion, cells were cultured on plain tissue culture polystyrene (TCP) or on coated well plates, which allowed only adhesion to embedded fibronectin or RGD peptides. We found that the expression of collagen I, which is involved in osteogenesis, and VEGF, a factor which stimulates angiogenesis, increased as a result of short‐term mechanical integrin stress. Whereas, collagen I expression was stimulated by mechanical forces when the cells were cultured on fibronectin and RGD peptides but not on TCP, VEGF expression was enhanced by physical stimulation on TCP. The study further revealed that magnetic forces enhanced Sox 9 expression, a marker of chondrogenesis, and reduced the expression of ALP. Concerning the intracellular mechanisms involved, we found that the expression of VEGF induced by physical forces depended on Akt activation. Together, the results implicate that biological functions of MSC can be stimulated by integrin‐mediated mechanical forces and a magnetic field. However, the responses of cells depend strongly on the substrate to which they adhere and on the cross‐talk between integrin‐mediated signals and soluble factors. J. Cell. Biochem. 111: 1586–1597, 2010. © 2010 Wiley‐Liss, Inc.