bluuebunny/crossref_metadata_2025_split huggingface dataset:
Keratin hydrogels support the sustained release of bioactive ciprofloxacin
Authors: Saul Justin M. , Ellenburg Mary D. , de Guzman Roche C. , Dyke Mark Van 
Published: June 2011

AbstractKeratins are naturally derived proteins that can be fabricated into several biomaterial forms including hydrogels. These materials are a potential polymeric system for several tissue engineering and regenerative medicine applications due to their ability to support cell attachment, proliferation, and migration. However, little is known regarding their ability to support sustained release of therapeutic agents. This report describes the use of keratin hydrogels for sustained release of the antibiotic ciprofloxacin, which may prove useful to traumatic injury applications that would benefit from materials promoting tissue regeneration while also preventing acute infection. Hydrogels were formed from keratins obtained by oxidative extraction and known as keratose. We found that keratose hydrogels released ∼60% of loaded ciprofloxacin over the first 10 days and that continued release was detectable over the course of 3 weeks. Released ciprofloxacin was bioactive, inhibiting growth of Staphylococcus aureus for 23 days in vitro and for 2 weeks in a mouse subcutaneous model. The rate of ciprofloxacin release was highly correlated with degradation of the keratin hydrogel and not consistent with simple diffusion. Further experiments indicated that ciprofloxacin binds to keratose through electrostatic interactions. These studies demonstrate the specific use of keratose hydrogels for the release of antibiotic and the potential for the more general use of this material in tissue engineering and regenerative medicine applications. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
Studies on the hemocompatibility of bacterial cellulose
Authors: Andrade Fábia K. , Silva João Pedro , Carvalho Manuela , Castanheira Elisabete M. S. , Soares Raquel , Gama Miguel 
Published: June 2011

AbstractAmong the strategies to improve a material's hemocompatibility, pre‐coating with the tripeptide Arg‐Gly‐Asp (RGD) is used to favor endothelialization thus lowering thrombogenicity. The blood compatibility of native and RGD‐modified bacterial cellulose (BC) was studied in this work for the first time. The plasma recalcification time and whole blood clotting results demonstrate the hemocompatibility of BC. A significant amount of plasma protein adsorb to BC fibres, however, according to analysis by intrinsic tryptophan fluorescence techniques when albumin, γ‐globulin, and fibrinogen from pure protein solutions adsorb to BC do not undergo detectable conformational modifications. Human microvascular endothelial cells cultured on RGD‐modified BC readily form a confluent cell layer, inhibiting the adhesion of platelets. As a general conclusion, both native and RGD‐modified BCs may be classified as hemocompatible materials. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
Characteristics of hydroxyapatite film formed on human enamel with the powder jet deposition technique
Authors: Akatsuka Ryo , Sasaki Keiichi , Zahmaty Mohammed Saeed Sepasy , Noji Miyoko , Anada Takahisa , Suzuki Osamu , Kuriyagawa Tsunemoto 
Published: June 2011

AbstractThis study aimed to create hydroxyapatite (HAp) film by powder jet deposition with manipulating the blasting nozzle above human enamel and to examine the microstructural and mechanical properties of the HAp film and the bonding strength at the interface between the HAp film and the enamel substrate. HAp particles calcinated at 1200°C with an average size of 4.7 μm were used. The HAp particles were mixed with carrier gas (N2) to form an aerosol flow and was accelerated and blasted from the nozzle onto the enamel substrate at room temperature and atmospheric pressure. To evaluate the microstructure, scanning electron microscope (SEM) images of the surface and cross section of the HAp films and a three‐dimensional profile of the HAp films were observed. To evaluate the mechanical properties, the micro‐Vickers hardness and the bonding strength of the HAp films to the enamel substrate were measured. The deposition area of the HAp film was over 3 × 4 mm. The average and maximum thickness were about 30 and 40 μm, respectively. No significant difference was observed between the hardness of the HAp film and the enamel (p > 0.05). The bonding strength of the HAp film was the same as the bonding strength between composite resin and enamel. Compared with previous reports, wider and thicker HAp film was created on the enamel substrate successfully. The HAp film, which has same hardness with enamel and same bonding strength to the enamel with composite resin, would be a candidate as dental restorative materials. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.
Gene modification with integrin‐linked kinase improves function of endothelial progenitor cells in pre‐eclampsia in vitro
Authors: Wang Fang , Wang Ying , Zhang Li , Zou Li 
Published: June 2011

AbstractIntegrin‐linked kinase (ILK), a multifunctional serine–threonine protein kinase, has been shown to have implications for the treatment of ischemia vascular diseases by promoting angiogenesis in various tissues. However, whether this kinase has therapeutic potential in pre‐eclampsia is not well studied. In this report, we determined the changes in the production and action of ILK on endothelial progenitor cells (EPCs) isolated from patients with pre‐eclampsia. The effects of ILK transfection on proliferation, migration, and angiogenesis of EPCs were investigated. We showed that EPCs transfected with the ILK gene expressed high levels of ILK protein and mRNA. Transfection with ILK also enhanced the proliferative, migratory, and angiogenic capabilities of EPCs, and promoted the production of VEGF. These results suggest that ILK gene transfection is an effective approach to augment angiogenic properties of EPCs in vitro and providing basis for clinical cell‐based gene therapy in patients with pre‐eclampsia. J. Cell. Biochem. 112: 3103–3111, 2011. © 2011 Wiley Periodicals, Inc.
Lessons learned from a long‐term psychoanalysis on the telephone
Authors:   Anonymous
Published: June 2011

AbstractThis article describes my 30+ years of psychoanalysis, the bulk of which has occurred on the telephone. I am publishing the article anonymously so that I can speak freely, without family or professional repercussions, about these vital topics. Of the myriad issues that have been addressed in the treatment, I have elected to focus in this article on separation‐individuation challenges, adult‐life trauma, career dynamics, silences in analysis, and the value of consultations and adjunctive treatments. The article focuses on the applicability of these themes to my personal life and their relevance to me as a clinician and clinical supervisor. © 2011 Wiley Periodicals, Inc. J Clin Psychol: In Session 67:1–10, 2011.
Glucocorticoid alternative effects on proliferating and differentiated mammary epithelium are associated to opposite regulation of cell‐cycle inhibitor expression
Authors: Hoijman Esteban , Rocha‐Viegas Luciana , Kalko Susana G. , Rubinstein Natalia , Morales‐Ruiz Manuel , Joffé Elisa Bal de Kier , Kordon Edith C. , Pecci Adali 
Published: June 2011

AbstractGlucocorticoids influence post‐natal mammary gland development by sequentially controlling cell proliferation, differentiation, and apoptosis. In the mammary gland, it has been demonstrated that glucocorticoid treatment inhibits epithelial apoptosis in post‐lactating glands. In this study, our first goal was to identify new glucocorticoid target genes that could be involved in generating this effect. Expression profiling, by microarray analysis, revealed that expression of several cell‐cycle control genes was altered by dexamethasone (DEX) treatment after lactation. Importantly, it was determined that not only the exogenous synthetic hormone, but also the endogenous glucocorticoids regulated the expression of these genes. Particularly, we found that the expression of cell cycle inhibitors p21CIP1, p18INK4c, and Atm was differentially regulated by glucocorticoids through the successive stages of mammary gland development. In undifferentiated cells, DEX treatment induced their expression and reduced cell proliferation, while in differentiated cells this hormone repressed expression of those cell cycle inhibitors and promoted survival. Therefore, differentiation status determined the effect of glucocorticoids on mammary cell fate. Particularly, we have determined that p21CIP1 inhibition would mediate the activity of these hormones in differentiated mammary cells because over‐expression of this protein blocked DEX‐induced apoptosis protection. Together, our data suggest that the multiple roles played by glucocorticoids in mammary gland development and function might be at least partially due to the alternative roles that these hormones play on the expression of cell cycle regulators. J. Cell. Physiol. 227: 1721–1730, 2012. © 2011 Wiley Periodicals, Inc.
Post‐transcriptional regulation of the Ras‐ERK/MAPK signaling pathway
Authors: Whelan Jarrett T. , Hollis Sarah E. , Cha Dong Seok , Asch Adam S. , Lee Myon‐Hee 
Published: June 2011

AbstractThe Ras‐ERK/MAP (Mitogen‐Activated Protein) kinase signaling pathway governs many cellular processes such as proliferation, differentiation, cell fate, homeostasis, and survival in all eukaryotes. Constitutive activation of the Ras‐ERK/MAPK signaling pathway often leads to promotion of abnormal cell growth and tumorigenesis. Although the regulation of the Ras‐ERK/MAPK signaling pathway by post‐translational modification has been well elucidated, post‐transcriptional regulations of this pathway are beginning to emerge in invertebrates and this work is extended to humans. In this review, we describe the conserved regulation of Ras‐ERK/MAPK signaling by RNA‐binding proteins (PUF, KH‐domain, HuR, and LARP) and microRNAs (let‐7 family miRNAs) and important implications for human diseases including cancers. J. Cell. Physiol. 227: 1235–1241, 2012. © 2011 Wiley Periodicals, Inc.
Native polyacrylamide electrophoresis in the presence of Ponceau Red to study oligomeric states of protein complexes
Authors: Dráb Tomáš , Kračmerová Jana , Tichá Ivana , Hanzlíková Eva , Tichá Marie , Liberda Jiří 
Published: June 2011

AbstractNative polyacrylamide electrophoresis in the presence of two reversible protein anionic stains (Ponceau S and Ponceau 2R) was used to study the oligomeric states of soluble proteins. A mild binding of the used protein stains to nondissociated protein oligomers imposed a charge shift on the proteins resulting into separation of protein species according to their size under physiological conditions. Adsorbed stains could be easily removed after electrophoresis by washing of polyacrylamide gel with buffer and protein complexes could be visualized either by the detection of their enzyme activity or by using a nonspecific protein stain. The specific detection of enzyme activity of glycosidases, lactate dehydrogenase, or phosphatases was shown as an example.
α,ω‐Functionalized Poly(2‐Oxazoline)s Bearing Hydroxyl and Amino Functions
Authors: Reif Michael , Jordan Rainer 
Published: June 2011

AbstractNovel α,ω‐functionalized amphiphilic lipopolymers are prepared that are composed of a proximal lipid moiety and a hydrophilic poly(2‐oxazoline)‐based (POx) polymer chain. The synthesis begins from bifunctional lipoinitiators, which are asymmetrically protected as tert.butyldiphenylsilyl (TBDPS) ethers, followed by cationic living ring‐opening polymerization of 2‐oxazolines in a one‐pot multistep reaction. This results in polymers with defined terminal end groups and narrow molar mass distributions. All protective groups involved can be readily cleaved in a single step reaction keeping the structure of the polymer intact, giving access to (lipo)polymers with a variety of defined identical or chemical orthogonal α,ω‐functionalities. The synthetic strategy is a versatile tool for the preparation of defined polymer–drug or polymer–protein conjugates or asymmetric functionalized model lipid membranes for the quantitative study of membrane‐associated phenomena such as transmembrane transport and cell adhesion/recognition.
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Targeting COPD: advances on low‐molecular‐weight inhibitors of human neutrophil elastase
Authors: Lucas Susana D. , Costa Elsa , Guedes Rita C. , Moreira Rui 
Published: June 2011

AbstractChronic obstructive pulmonary disease (COPD) is a major increasing health problem and the World Health Organization (WHO) reports COPD as the fifth leading cause of death worldwide. COPD refers to a condition of inflammation and progressive weakening of the structure of the lung as well as irreversible narrowing of the airways. Current treatment is only palliative and no available drug halts the progression of the disease. Human neutrophil elastase (HNE) is a serine protease, which plays a major role in the COPD inflammatory process. The protease/anti‐protease imbalance leads to an excess of extracellular HNE hydrolyzing elastin, the structural protein that confers elasticity to the lung tissue. Although HNE was identified as a therapeutic target for COPD more than 30 years ago, only Sivelestat (ONO‐5046), an HNE inhibitor from Ono Pharmaceutical, has been approved for clinical use. Nevertheless, Sivelestat is only approved in Japan and its development in the USA was terminated in 2003. Other inhibitors in pre‐clinical or phase I trials were discontinued for various reasons. Hence, there is an urgent need for low‐molecular‐weight synthetic elastase inhibitors and the present review discusses the recent advances on this field covering acylating agents, transition‐state inhibitors, mechanism‐based inhibitors, relevant natural products, and major patent disclosures.
Design of low‐timing‐jitter, stable picosecond optical‐pulse source using an uncooled gain‐switched Fabry–Perot semiconductor laser with external continuous‐wave light injection
Authors: Liu Yuanshan , Zhang Jian‐Guo 
Published: June 2011

AbstractThis paper presents the design of a low‐timing‐jitter, stable picosecond optical‐pulse source, which is based on a low‐cost, uncooled Fabry–Perot (FP) semiconductor laser in the gain‐switching operation. The wavelength of the designed laser can be tuned from 1538 to 1554 nm under the condition of external continuous‐wave (CW) light injection. The relationship between injection light power and timing jitter or between injection light power and pulse width of this gain‐switched laser is studied experimentally. Our results show that the optical pulses with widths about 28 and 19 ps at the repetition frequencies of 2.5 and 5 GHz, respectively, are produced by an uncooled gain‐switched FP semiconductor laser with timing jitter ≤600 fs, when the injection power and wavelength of an external CW light are appropriately chosen. The use of a 500‐m dispersion‐compensation fiber can easily compress the optical pulses from 26 to 7.7 ps. Moreover, the stability of an uncooled gain‐switched FP laser is experimentally investigated, and a stable optical‐pulse train at 5 GHz can be feasibly produced over 7 h of continuously working. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26176
A compact multiband antenna for WLAN and WiMAX applications
Authors: Wang Chen , Xu Ping , Li Bo , Yan Ze‐Hong 
Published: June 2011

AbstractA compact printed monopole antenna applied to wireless local area network (WLAN) and worldwide interoperability for microwave access (WiMAX) applications is proposed. By combining a C‐shaped strip and two L‐shaped strips, fed by a 50‐Ω microstrip transmission line, multiple impedance bandwidths covering WLAN/2.4/5.2/5.8 and WiMAX/3.5 are obtained. Further, the proposed antenna occupies a small size of 35 × 20 mm2, and has good radiation characteristic and peak gains to be 2.77, 2.92, and 3.04 dBi at 2.45, 3.5, and 5.5 GHz, respectively. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26183
Impedance matching and spurious‐response suppression in stepped‐impedance low pass filters
Authors: Oraizi Homayoon , Seyyed‐Esfahlan Mehdi 
Published: June 2011

AbstractIn this article, the method of least squares is used to design a stepped‐impedance low pass filter with arbitrary specifications on the pass, transition, and stop bands, having different input and output impedances.The equivalent circuits of the line sections and step discontinuities are used (with appropriate dispersion relations) to determine the filter transmission matrix and the scattering parameters, which are then used to construct an error function. Its minimization is performed by the combination of particle swarm optimization and conjugate gradient methods. The conversion of lumped filter designs, by Butterworth, Chebyshev, and elliptic methods to the equivalent microstrip filters (namely conversion of L and C components to strip lines) may be difficult to realize. The proposed method of least squares procedure leads to the design characteristics having sharper transition bands and a wider pass band. The rectangular split ring resonators, as patterned ground plane, will remove the spurious responses in the stop band and actually increase the stop bandwidth. The results obtained by full‐wave simulation softwares, fabrication, and measurements verify the proposed method for low pass stepped‐impedance filter design. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26184
Design of a PIFA with parasitic F‐element miniaturized antenna assembly for lower band ultra‐wideband and IEEE 802.11a applications
Authors: See Chan H. , Abd‐Alhameed Raed A. , Hraga H.I. , Elfergani Issa T. E. , Abusitta Musa M. , Adnan S. 
Published: June 2011

AbstractIn this article, a planar inverted‐FF antenna with suspended rectangular feeding mechanism is presented for lower band ultra‐wideband and IEEE 802.11a wireless local area network applications. By manipulating the optimum strong electromagnetic coupling distance between driven and parasitic F‐shaped radiators on a small ground plane, two closely separate resonances can be merged to form a wide impedance bandwidth of 73.7% (3.0–6.5 GHz) at |S11| < −10 dB. The overall occupied antenna volume is 30 × 15 × 3 mm3 to satisfy the size requirement of modern wireless devices. The impact of the different antenna geometry parameters on the antenna performance is studied. The corresponding measured radiation patterns, gains and group delays are given to fully characterize the performance of this antenna. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26189
Designing a chip antenna with dual‐band by a material‐loading technique
Authors: Huang Chi‐Fang , Yeh Pei‐Lan 
Published: June 2011

AbstractBased on a manufacturing technology of traditional machinery, a design method of chip antenna is proposed for the dual‐band operation of GSM wireless communication. This new approach emphasizes several skills for the optimal frequency tuning for this kind of antenna with material‐loaded meander line. Results of both simulation and measurement are shown to validate this design technique. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26190
Dual‐band MIMO antenna using polarization diversity for 4G mobile handset application
Authors: Han Minseok , Choi Jaehoon 
Published: June 2011

AbstractA dual‐band multiple‐input multiple‐output (MIMO) antenna using polarization diversity is proposed for a 4G mobile handset application.The proposed MIMO antenna consists of two printed dual‐band PIFAs with a slotted strip. Input port 1 of the first PIFA is orthogonally disposed with respect to input port 2 of the second PIFA. The orthogonal placement of the two feed points ensures that the dominant polarization of each of the PIFAs is opposite to each other, resulting in a good isolation performance. The proposed MIMO antenna has an isolation of ∼17 dB at LTE band 13, and the envelope correlation coefficient (ECC) of the two antennas is always lower than 0.4 over all of LTE band 13. To evaluate the performance of the proposed antenna, key performance parameters such as the total efficiency, ECC, mean effective gain (MEG), MEG ratio, and actual diversity gain are analyzed. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26197
Multiband MIMO antenna using orthogonally polarized dipole elements for mobile communications
Authors: Han Minseok , Choi Jaehoon 
Published: June 2011

AbstractA multiband multiple‐input multiple‐output (MIMO) antenna using polarization diversity for next generation mobile communication is proposed.The MIMO antenna consists of two dual‐band (0.75 GHz/2.5 GHz) dipole antennas with a separation distance of 0.8 mm. One dipole is etched on the top of a two‐layered dielectric substrate with a dimension of 80 × 80 mm2 and the other is printed on the bottom side of the same substrate. They are located orthogonal to one another. Even though the two stacked dipoles are separated by only 0.8 mm, the port‐to‐port isolation is maintained at greater than 20 dB, and the envelope correlation coefficient (ECC) of the two antennas is always lower than 0.2 over the entire frequency band. To evaluate the performance of the proposed antenna, key performance parameters such as the total efficiency, ECC, mean effective gain (MEG), MEG ratio, and actual diversity gain are analyzed. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26198
Wide stopband compact microstrip lowpass filter using circular ring resonator and split ring resonators
Authors: Jahromi Mahdi Naghshvarian 
Published: June 2011

AbstractIn this contribution, a microstrip low‐pass filter with a wide stop‐band using split ring resonators and a circular ring resonator is introduced. The passband ripple of proposed filter was measured 0.6 dB and minimum insertion loss of passband is about 0.75 dB at 0.5 GHz frequency. Moreover, the 1.0 dB cut off frequency of proposed filter is 1.45 GHz in measurement. Designing this type of low‐pass filter requires a high impedance transmission‐line. However, the microstrip line width cannot be taken less than 0.2 mm in practical implementation. This causes to a small mismatch in passband. Furthermore, these results show wide stop‐band up to 9.0 GHz with more than 30 dB rejection. The dimensions of proposed compact filter are about 12.5×30 mm2 (excluding extra substrate width). The proposed filter is a microstrip filter and can compete with defected ground structure (DGS) or defected microstrip structures lowpass filters in miniaturizing and wide stopband. However, the DGS or DGS filters have some problems in manufacturing applications such as radiation fields from defected structures and requiring a specific box. In addition, a distributed equivalent circuit model based on geometrical filer parameters is introduced, and good agreement can be seen between EM simulation and distributed equivalent circuit model up to 6.0 GHz frequency. © 2011 Wiley Periodicals, Inc. Microwave Opt Technol Lett, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26203