bluuebunny/crossref_metadata_2025_split huggingface dataset:
Awake venovenous extracorporeal membrane oxygenation and survival
Authors: Rottmann Felix A. , Zotzmann Viviane , Supady Alexander , Noe Christian , Wengenmayer Tobias , Staudacher Dawid L. 
Published: April 2024

ObjectivesDeep sedation on the ICU is linked to poor outcome. This study investigated the link between Richmond Agitation-Sedation Scale (RASS) and outcome in venovenous extracorporeal membrane oxygenation (V-V ECMO).MethodsWe performed a secondary analysis of a single-center V-V ECMO cohort. RASS was used as a surrogate measure of sedation depth, patients with a score ≥ −1 were considered awake. V-V ECMO durations below 24 h were excluded. Primary endpoint was 30-day survival. Secondary endpoints were hospital survival and weaning from both ventilator and ECMO therapy.ResultsA total of 343 patients were reanalyzed. The median age was 55 years and 52.2% (179/343) survived for 30 days after ECMO cannulation. Median duration of ECMO was 7.9 (4.7–15.0) days and the median duration of mechanical ventilation after ECMO cannulation was 11.8 (6.7–23.8) days.In the whole cohort, median RASS on day one and seven after ECMO were − 4 (−4 to −1) and − 3 (−4 to 0), respectively. ECMO survivors consistently had significantly higher RASS scores during the first 7 days of ECMO compared to non-surviving patients (p &lt; 0.01). On day two after ECMO, survival of awake patients (i.e., RASS ≥-1) was significantly better compared to sedated [i.e., RASS −4 to −2; OR 2.20 (1.28–3.71), p &lt; 0.01] or unresponsive patients [i.e., RASS -5; OR 2.27 (1.15–4.64), p = 0.02]. The survival benefit of awake ECMO was consistent from day two to seven. Patients awake at least once during ECMO showed higher 30-day survival rates [64.4% vs. 39.6%, OR 2.75 (1.77–4.24), p &lt; 0.01].ConclusionIn this retrospective study, awake patients on V-V ECMO showed higher 30-day survival rates compared to sedated or unresponsive patients. These data should encourage further research on awake V-V ECMO.
A 3D pseudo-continuous arterial spin labeling study of altered cerebral blood flow correlation networks in mild cognitive impairment and Alzheimer's disease
Authors: Li Meng , Zhu Tianjia , Kang Yan , Qi Shouliang 
Published: April 2024

ObjectiveTo investigate the abnormalities of the three-dimensional pseudo-continuous arterial spin labeling (3D PCASL) based cerebral blood flow (CBF) correlation networks in mild cognitive impairment (MCI) and Alzheimer's disease (AD).Methods3D PCASL images of 53 cognitive normal (CN) subjects, 43 subjects with MCI, and 30 subjects with AD were acquired from the Alzheimer's Disease Neuroimaging Initiative (ADNI) database. Whole-brain CBF maps were calculated using PCASL and proton density-weighted images (PDWI). The 246 regional CBF values, including the cortex and subcortex, were obtained after registering the Brainnetome Atlas to the individual CBF maps. The Pearson correlation coefficient between every two regions across subjects was calculated to construct the CBF correlation network. Then the topologies of CBF networks with regard to global properties (global network efficiency, clustering coefficient, characteristic path length, and small-world properties), hub regions, nodal properties (betweenness centrality, BC), and connections were compared among CN, MCI, and AD. Significant changes in the global and nodal properties were observed in the permutation tests, and connections with significant differences survived after the z-statistic and false discovery rate (FDR) correction.ResultsThe CBF correlation networks of CN, MCI, and AD all showed small-world properties. Compared with CN, global efficiency decreased significantly in AD. Significant differences in nodal properties and a loss of hub regions are noted in the middle temporal lobe in both MCI and AD. In the frontal lobe, BC is reduced in MCI while it is increased in the occipital lobe in AD. The identified altered hub regions with significant differences in MCI and AD were mainly distributed in the hippocampus and entorhinal cortex. In addition, disrupted hub regions in AD with significantly decreased connections were mainly found in the precuneus/posterior cingulate cortex (PCC) and hippocampus-cortical cortex.ConclusionsNoninvasive 3D PCASL-based CBF correlation networks are capable of showing changes in topological organization in subjects with MCI and AD, and the observed disruption in the topological organization may underlie cognitive decline in MCI and AD.
Factors predicting recanalization following stent-assisted coil embolization of unruptured intracranial aneurysms with long-term follow-up
Authors: Won Yu Deok , Kim Young Deok , Ban Seung Pil , Kwon O-Ki 
Published: April 2024

ObjectiveStents have been widely used for coil embolization for intracranial aneurysms. Few studies have analyzed the risk factors of recanalization through long-term follow-up observation of only stent-assisted coiling. We analyzed the risk factors for recanalization through long-term observations.MethodsA total number of 399 unruptured aneurysms treated by stent-assisted coil embolization between 2003 and 2016 in a single institution were analyzed for determining the factors associated with recanalization including the patient characteristics, aneurysms, and procedural variables. All patients underwent angiographic follow-up with digital subtraction angiography or magnetic resonance angiography at 24 months or more following the procedure.ResultsRecanalization occurred in 8%. The mean time for the recanalization was 21.1 ± 14.0 months (range, 5–51 months). The receiver operating characteristic curve analysis indicated areas under the curve for a maximum aneurysm size of 0.773 (cut-off, 6.415 mm). Multivariate analysis revealed that the maximum aneurysm size and parent artery curvature at which the aneurysm developed were significantly associated with recanalization. In parent artery curvature, the bifurcation group (OR, 9.02; 95% CI, 2.53–32.13; p = 0.001) and the convex group (OR, 3.68; 95% CI, 1.17–11.50; p = 0.025) were independent predictors of recanalization compared with the straight group.ConclusionThe maximum aneurysm size and parent artery curvature are risk factors associated with recanalization in stent-assisted coil embolization.
Unilateral biportal endoscopy vs. open decompression for lumbar epidural lipomatosis-cohort study using a prospective registry
Authors: Tan Bing , Zheng Yu-hao , Lei Chao , Ouyang Jian-yuan , Wen Yan-bo , Shi Zhuo-hua , Yang Qi-Yuan 
Published: April 2024

ObjectiveThis study aimed to compare the outcomes of unilateral biportal endoscopy, unilateral laminectomy bilateral decompression (UBE-ULBD), and open lumbar decompression (OLD) in patients with lumbar epidural lipomatosis (LEL).MethodsThis prospective observational study was conducted from March 2019 to May 2022 and encompassed 33 patients with LEL who underwent lumbar decompression. The study included 15 cases of UBE-ULBD decompression and 18 cases of open decompression, which were followed up for 1 year. The baseline characteristics, initial clinical manifestations, and surgical details [including estimated blood loss (EBL) and preoperative complications] of all patients were recorded. Radiographic evaluation included the cross-sectional area (CSA) of the thecal sac and paraspinal muscles on MRI. Clinical results were analyzed using the Short Form-36 Score (SF-36), the Numeric Pain Rating Scale (NRS) for lumbar and leg pain, creatine kinase, the Roland and Morris Disability Questionnaire (RMDQ), and the Oswestry Disability Index (ODI).ResultsThe dural sac CSA increased considerably at the 1-year postoperative follow-up in both groups (p &lt; 0.001). The operative duration in the OLD group (48.2 ± 7.2 min) was shorter than that in the UBE-ULBD group (67.7 ± 6.3 min, p &lt; 0.001). The OLD group (97.2 ± 19.8 mL) was associated with more EBL than the UBE-ULBD group (40.6 ± 13.6 mL, p &lt; 0.001). The duration of hospitalization in the OLD group (5.4 ± 1.3 days) was significantly longer compared with the UBE-ULBD group (3.5 ± 1.2 days, p &lt; 0.01). The SF-36, NRS, RMDQ, and ODI scores improved in both groups postoperatively (p &lt; 0.001). Serum creatine kinase values in the UBE-ULBD group (101.7 ± 15.5) were significantly lower than those in the OLD group (330.8 ± 28.1 U/L) 1 day after surgery (p &lt; 0.001). The degree of paraspinal muscle atrophy in the UBE-ULBD group (4.81 ± 1.94) was significantly lower than that in the OLD group (12.15 ± 6.99) at 1 year (p &lt; 0.001).ConclusionUBE-ULBD and OLD demonstrated comparable clinical outcomes in treating LEL. However, UBE-ULBD surgery was associated with shorter hospital stays, lower rates of incision infection, lighter paravertebral muscle injury, and lower EBL than OLD surgery. Consequently, UBE-ULBD can be recommended in patients with LEL if conservative treatment fails.
The artificial sweetener neotame negatively regulates the intestinal epithelium directly through T1R3-signaling and indirectly through pathogenic changes to model gut bacteria
Authors: Shil Aparna , Ladeira Faria Luisa Maria , Walker Caray Anne , Chichger Havovi 
Published: April 2024

IntroductionRecent studies have indicated considerable health risks associated with the consumption of artificial sweeteners. Neotame is a relatively new sweetener in the global market however there is still limited data on the impact of neotame on the intestinal epithelium or the commensal microbiota.MethodsIn the present study, we use a model of the intestinal epithelium (Caco-2) and microbiota (Escherichia coli and Enterococcus faecalis) to investigate how physiologically-relevant exposure of neotame impacts intestinal epithelial cell function, gut bacterial metabolism and pathogenicity, and gut epithelium-microbiota interactions.ResultsOur findings show that neotame causes intestinal epithelial cell apoptosis and death with siRNA knockdown of T1R3 expression significantly attenuating the neotame-induced loss to cell viability. Similarly, neotame exposure results in barrier disruption with enhanced monolayer leak and reduced claudin-3 cell surface expression through a T1R3-dependent pathway. Using the gut bacteria models, E. coli and E. faecalis, neotame significantly increased biofilm formation and metabolites of E. coli, but not E. faecalis, reduced Caco-2 cell viability. In co-culture studies, neotame exposure increased adhesion capacity of E. coli and E. faecalis onto Caco-2 cells and invasion capacity of E. coli. Neotame-induced biofilm formation, E.coli-specific Caco-2 cell death, adhesion and invasion was identified to be meditated through a taste-dependent pathway.DiscussionOur study identifies novel pathogenic effects of neotame on the intestinal epithelium or bacteria alone, and in co-cultures to mimic the gut microbiome. These findings demonstrate the need to better understand food additives common in the global market and the molecular mechanisms underlying potential negative health impacts.
A rapid and simple non-radioactive assay for measuring uptake by solute carrier transporters
Authors: Song Kunling , Zhang Longbin , Fu Xian , Li Linfeng , Zhu Gaolin , Wu Mingjun , Zhang Wei , He Jia , Zhu Sanyong , Dang Yongjun , Liu Jun-Yan , Chen Chang , Guo Zufeng 
Published: April 2024

Introduction: Solute carrier (SLC) transport proteins play a crucial role in maintaining cellular nutrient and metabolite homeostasis and are implicated in various human diseases, making them potential targets for therapeutic interventions. However, the study of SLCs has been limited due to the lack of suitable tools, particularly cell-based substrate uptake assays, necessary for understanding their biological functions and for drug discovery purposes.Methods: In this study, a cell-based uptake assay was developed using a stable isotope-labeled compound as the substrate for SLCs, with detection facilitated by liquid chromatography-tandem mass spectrometry (LC-MS/MS). This assay aimed to address the limitations of existing assays, such as reliance on hazardous radiolabeled substrates and limited availability of fluorescent biosensors.Results: The developed assay was successfully applied to detect substrate uptakes by two specific SLCs: L-type amino acid transporter 1 (LAT1) and sodium taurocholate co-transporting polypeptide (NTCP). Importantly, the assay demonstrated comparable results to the radioactive method, indicating its reliability and accuracy. Furthermore, the assay was utilized to screen for novel inhibitors of NTCP, leading to the identification of a potential NTCP inhibitor compound.Discussion: The findings highlight the utility of the developed cell-based uptake assay as a rapid, simple, and environmentally friendly tool for investigating SLCs’ biological roles and for drug discovery purposes. This assay offers a safer alternative to traditional methods and has the potential to contribute significantly to advancing our understanding of SLC function and identifying therapeutic agents targeting SLC-mediated pathways.
The antitumor action of endocannabinoids in the tumor microenvironment of glioblastoma
Authors: Tang Yi , Wang Maoru , Yu Jiangping , Lv Guangyao , Wang Yu , Yu Bin 
Published: April 2024

Approximately 80% of all malignant brain tumors are gliomas, which are primary brain tumors. The most prevalent subtype of glioma, glioblastoma multiforme (GBM), is also the most deadly. Chemotherapy, immunotherapy, surgery, and conventional pharmacotherapy are currently available therapeutic options for GBM; unfortunately, these approaches only prolong the patient’s life by 5 years at most. Despite numerous intensive therapeutic options, GBM is considered incurable. Accumulating preclinical data indicate that overt antitumoral effects can be induced by pharmacologically activating endocannabinoid receptors on glioma cells by modifying important intracellular signaling cascades. The complex mechanism underlying the endocannabinoid receptor-evoked antitumoral activity in experimental models of glioma may inhibit the ability of cancer cells to invade, proliferate, and exhibit stem cell-like characteristics, along with altering other aspects of the complex tumor microenvironment. The exact biological function of the endocannabinoid system in the development and spread of gliomas, however, is remains unclear and appears to rely heavily on context. Previous studies have revealed that endocannabinoid receptors are present in the tumor microenvironment, suggesting that these receptors could be novel targets for the treatment of GBM. Additionally, endocannabinoids have demonstrated anticancer effects through signaling pathways linked to the classic features of cancer. Thus, the pharmacology of endocannabinoids in the glioblastoma microenvironment is the main topic of this review, which may promote the development of future GBM therapies.
“Eco-caring together” pro-ecological group-based community interventions and mental wellbeing: a systematic scoping review
Authors: Baker Kane , Chioran Bianca , Marks Elizabeth 
Published: April 2024

BackgroundPoor mental wellbeing is a challenge for societies across the world, as is the increasing threat of climate change, and emerging evidence suggests these challenges are interrelated. Green and social prescribing of non-clinical interventions hold promise as a cost-effective and widely accessible way to improve wellbeing, and interest is growing in whether pro-ecological communal activities have mutual benefits for both people and the planet.ObjectivesCommunal pro-ecological activities are growing in popularity, and research is gathering pace into whether participation influences mental wellbeing. The present systematic review scopes the existing evidence base to explore what is being done, what is being found, and what additional research is required.MethodsElectronic databases (PsychNET, PubMed, Scopus, and Web of Science) were searched for studies that involved groups of people undertaking pro-ecological activities, where components of mental wellbeing were assessed. Eligibility criteria were purposely broad, including all study designs and participants across the lifespan.ResultsThirty-seven eligible studies were identified. Nearly half of the studies used mixed-method designs, and most studies used surveys or interviews to evaluate outcomes. Most pro-ecological activities involved planting vegetation, and habitat creation, maintenance, or restoration. Methodological quality varied considerably. Among the perceived therapeutic mechanisms reported, the social elements of the interventions were prominent.DiscussionCoherent synthesis of the current evidence base is challenging given the heterogeneous range of methods, samples, and interventions within the studies. However, the results here demonstrate promise that with future research and better methodological rigor, pro-ecological group-based interventions hold the potential to improve mental wellbeing and influence sustainable behavior.Systematic review registrationhttps://osf.io/vmpr6/.
Effects of an online mindfulness-based program for parents of children with attention deficit/hyperactivity disorder: a pilot, mixed methods study
Authors: Lo Herman Hay Ming , Lam Jason , Zhang Zoe Ji-wen , Cheung Marsena , Chan Stanley Kam Chung , Wong Elisabeth Wai Yin , Bögels Susan M. , Chui Wing Hong 
Published: April 2024

ObjectivesOnline mindfulness-based program (MBP) for parents and families especially in clinical population is limited. Engagement and significant dropout are major issues in MBP implementation. This pilot study examined the effects of an online mindfulness-based program (MBP) on parents of children with Attention Deficit/Hyperactivity Disorder (ADHD).MethodsA mixed methods study was applied to evaluate the effects of the MBP. A total of 43 parents were recruited and were randomly assigned into intervention group and waitlist control group. The online MBP lasted for 28 days, including 20 psychoeducation videos, homework audio guidance, and four instructor-led online group meetings. Purposive sampling was used to recruit parents who completed the program to share their experiences and suggestions for improving the program in semi-structured online interviews.ResultsQuantitative data showed that participants from the online MBP reported a medium to large effect on the reduction of child ADHD symptoms. In semi-structured interviews, participants reported positive experiences in their help seeking intention, and personal changes, such as emotion regulation and quality attention to their children. Participants further made suggestions for improvement.ConclusionsThe effect of online MBP is promising, and the program should be conducted. A large scale randomized controlled trial should be conducted to investigate the effects of MBP in clinical populations.Clinical trial registrationClinicalTrials.gov NCT05480423.
Examining the effectiveness of a training program on improving knowledge, functional skills, and attitude in natural disaster volunteers
Authors: Amini Fereshteh , Hidarnia Alireza , Ghofranipour Fazlollah , Motlagh Mohammad Esmaiel 
Published: April 2024

IntroductionDisaster relief volunteers must have certain psychological and cognitive characteristics. Therefore, the present study aimed to investigate the effectiveness of the training program on improving knowledge, functional skills, and attitude among disaster volunteers.MethodsA quasi-experimental study was conducted in 2023 in Iran, including an intervention and control group and follow-ups. Ninety health volunteers were randomly selected and divided into two groups of 45. The experimental group received the training program for an hour a week for three months. The control group received no intervention. The participants of both groups completed the disaster preparedness questionnaire at the pre-test, post-test, and one and three months after the intervention. Data was analyzed using SPSS “version 26” software in the methods section.ResultsThe intervention effect were significant in increasing the volunteers’ functional skills (F = 3.75), knowledge (F = 166.50), and attitude (F = 1.74), all in (p = 0.001). According to the results, this effect was stable over time for functional skills (F = 1.83) and knowledge (F = 18.04) all in (p &lt; 0.05).ConclusionTraining programs can help improve skills, attitudes and knowledge in natural disaster volunteers. Researchers interested in the field of natural disaster relief, particularly health researchers, could consider further examining the aforementioned topics in their studies.
Analysis of the situations and influencing factors of public anxiety in China: based on Baidu index data
Authors: Xie Tiantian , Huang Zetao , Tan Yue , Tan Tao 
Published: April 2024

BackgroundAnxiety disorders have emerged as one of the most prevalent mental health problems and health concerns. However, previous research has paid limited attention to measuring public anxiety from a broader perspective. Furthermore, while we know many factors that influence anxiety disorders, we still have an incomplete understanding of how these factors affect public anxiety. We aimed to quantify public anxiety from the perspective of Internet searches, and to analyze its spatiotemporal changing characteristics and influencing factors.MethodsThis study collected Baidu Index from 2014 to 2022 in 31 provinces in mainland China to measure the degree of public anxiety based on the Baidu Index from 2014 to 2022. The spatial autocorrelation analysis method was used to study the changing trends and spatial distribution characteristics of public anxiety. The influencing factors of public anxiety were studied using spatial statistical modeling methods.ResultsEmpirical analysis shows that the level of public anxiety in my country has continued to rise in recent years, with significant spatial clustering characteristics, especially in the eastern and central-southern regions. In addition, we constructed ordinary least squares (OLS) and geographically weighted regression (GWR) spatial statistical models to examine the relationship between social, economic, and environmental factors and public anxiety levels. We found that the GWR model that considers spatial correlation and dependence is significantly better than the OLS model in terms of fitting accuracy. Factors such as the number of college graduates, Internet traffic, and urbanization rate are significantly positively correlated with the level of public anxiety.ConclusionOur research results draw attention to public anxiety among policymakers, highlighting the necessity for a more extensive examination of anxiety issues, especially among university graduates, by the public and relevant authorities.
Prehospital use of point-of-care tests by community health workers: a scoping review
Authors: Ebbs Daniel , Taricia Max , Funaro Melissa C. , O’Daniel Maggie , Cappello Michael 
Published: April 2024

IntroductionPoint-of-Care Tests (POCTs) are utilized daily in resource abundant regions, however, are limited in the global south, particularly in the prehospital setting. Few studies exist on the use of non-malarial POCTs by Community Health Workers (CHWs). The purpose of this scoping review is to delineate the current diversity in and breadth of POCTs evaluated in the prehospital setting.MethodsA medical subject heading (MeSH) analysis of known key articles was done by an experienced medical librarian and scoping searches were performed in each database to capture “point of care testing” and “community health workers.” This review was guided by the PRISMA Extension for scoping reviews.Results2735 publications were returned, 185 were nominated for full-text review, and 110 studies were confirmed to meet study criteria. Majority focused on malaria (74/110; 67%) or HIV (25/110; 23%); 9/110 (8%) described other tests administered. Results from this review demonstrate a broad geographic range with significant heterogeneity in terminology for local CHWs.ConclusionThe use of new POCTs is on the rise and may improve early risk stratification in limited resource settings. Current evidence from decades of malaria POCTs can guide future implementation strategies.