bluuebunny/crossref_metadata_2025_split huggingface dataset:
Defluorination of monofluorinated alkane by Rhodococcus sp. NJF-7 isolated from soil
Authors: Yan Meng , Gao Zhaozhao , Xiang Xingjia , Wang Qing , Song Xin , Wu Yucheng , Löffler Frank E. , Zeng Jun , Lin Xiangui 
Published: June 2024

AbstractMicrobial degradation of fluorinated compounds raised significant attention because of their widespread distribution and potential environmental impacts. Here, we report a bacterial isolate, Rhodococcus sp. NJF-7 capable of defluorinating monofluorinated medium-chain length alkanes. This isolate consumed 2.29 ± 0.13 mmol L− 1 of 1-fluorodecane (FD) during a 52 h incubation period, resulting in a significant release of inorganic fluoride amounting to 2.16 ± 0.03 mmol L− 1. The defluorination process was strongly affected by the initial FD concentration and pH conditions, with lower pH increasing fluoride toxicity to bacterial cells and inhibiting enzymatic defluorination activity. Stoichiometric conversion of FD to fluoride was observed at neutral pH with resting cells, while defluorination was significantly lower at reduced pH (6.5). The discovery of the metabolites decanoic acid and methyl decanoate suggests that the initial attack by monooxygenases may be responsible for the biological defluorination of FD. The findings here provide new insights into microbial defluorination processes, specifically aiding in understanding the environmental fate of organic semi-fluorinated alkane chemicals.
Reproductive chemical database: a curated database of chemicals that modulate protein targets regulating important reproductive biological processes
Authors: Cao Yuedi , Tian Geng G. , Hong Xiaokun , Lu Qing , Wei Ting , Chen Hai-Feng , Wu Ji 
Published: June 2024

AbstractRecent studies have shifted the spotlight from adult disease to gametogenesis and embryo developmental events, and these are greatly affected by various environmental chemicals, such as drugs, metabolites, pollutants, and others. Growing research has highlighted the critical importance of identifying and understanding the roles of chemicals in reproductive biology. However, the functions and mechanisms of chemicals in reproductive processes remain incomplete. We developed a comprehensive database called the Reproductive Chemical Database (RCDB) (https://yu.life.sjtu.edu.cn/ChenLab/RCDB) to facilitate research on chemicals in reproductive biology. This resource is founded on rigorous manual literature extraction and precise protein target prediction methodologies. This database focuses on the delineation of chemicals associated with phenotypes, diseases, or endpoints intricately associated with four important reproductive processes: female and male gamete generation, fertilization, and embryo development in human and mouse. The RCDB encompasses 93 sub-GO processes, and it revealed 1447 intricate chemical–biological process interactions. To date, the RCDB has meticulously cataloged and annotated 830 distinct chemicals, while also predicting 614 target proteins from a selection of 3800 potential candidates. Additionally, the RCDB offers an online predictive tool that empowers researchers to ascertain whether specific chemicals play discernible functional roles in these reproductive processes. The RCDB is an exhaustive, cross-platform, manually curated database, which provides a user-friendly interface to search, browse, and use reproductive processes modulators and their comprehensive related information. The RCDB will help researchers to understand the whole reproductive process and related diseases and it has the potential to promote reproduction research in the pharmacological and pathophysiological areas.
Graphical Abstract
The importance of vision care for displaced populations: lessons from Israeli evacuees
Authors: Ben-Eli Hadas , Chowers Itay , Gordon-Shaag Ariela 
Published: June 2024

Abstract
Background
This study explores vision care priorities and coping mechanisms for Israeli evacuees and following the October 7th, 2023, attack by Hamas, which displaced 150,000 individuals, with about 15,000 being evacuated to the Dead Sea area. Faced with minimal health care infrastructure in the Dead Sea area and often lacking personal belongings, including eyeglasses and ocular medicine, these evacuees confronted significant vision care challenges. This context sets the stage for investigating the emergency vision care needs and solutions for populations affected by conflict and displacement.

Methods
In response to this crisis, a consortium led by Hadassah Academic College’s Department of Optometry and the Dept. of Ophthalmology at Hadassah Medical Center established ophthalmic clinics in the Dead Sea region. These clinics offered comprehensive vision care services, including refractive and vision examinations, ophthalmological assessments, ocular imaging, and provision of free glasses. The setup included multiple stations for different vision tests, staffed by an interdisciplinary team of professionals. The study analyzes the effectiveness of these clinics, patient flow challenges, and the psychological impact of vision care in a crisis setting.

Results
Approximately 800 evacuees received examinations, with around 700 pairs of glasses distributed. Notable cases included emergency referrals for serious conditions and instances where glasses served as psychological support. The operation highlighted the necessity of vision care during crises and its potential psychological and social implications. The clinics successfully provided immediate vision care, but challenges in patient flow and insufficient electronic medical record integration were noted. The experience underscores the importance of prepared eye care interventions in crises. Recommendations for health policy decision-makers include establishing a national emergency vision care network, developing standardized treatment protocols, training local health workers, and raising public awareness about eye health in emergencies.

Conclusions
The consortium’s effort in providing urgent vision care to evacuees from the Hamas attack on Israel demonstrates the critical role of rapid, organized eye care in crisis situations. Vision care, along with hearing and mobility, is often overlooked during evacuations but is vital for the well-being and survival of evacuees, especially under trying circumstances. This project serves as a model for future humanitarian interventions, emphasizing the importance of addressing overlooked healthcare issues once the immediate crisis has passed, and the need for strategic planning in health care policy for similar emergency scenarios.
Incidence of controlled water restriction on density and chemical profiles in tree rings of Araucaria araucana seedlings
Authors: Papú Sofía , Ortega-Rodriguez Daigard Ricardo , Roig Fidel Alejandro , Navas Florencia 
Published: June 2024

Abstract
Background
Dehydration of plant tissues caused by water stress affects the dynamics of the lateral cambium, the rate of cell division and differentiation in cell lumen size, wall thickness and wall chemical properties. Based on evidence that ongoing climate change projects longer and more intense water stress conditions, forest dynamics and decline are predicted to be affected by increases in drought intensity and frequency. To verify the interaction of these phenomena, we described the modifications in the anatomy and mineral contents of annual growth rings of Araucaria araucana seedlings subjected to water stress treatments, using X-ray densitometry and X-ray fluorescence techniques.

Results
Severe water stress conditions during the growing period produced narrower tree rings, with reduced cell lumen size (5–7 µm) and higher tracheid reinforcement factor values, but with lower wood density. Plants subjected to moderate water stress generated intra-annual density fluctuations coinciding with periods of decreased soil moisture. Under the precept that the essential nutrients play a relevant role in the functioning of trees, we found evidence of element allocation and concentration in response to drought. Calcium and phosphorus concentration increased significantly as stress becomes more severe, but with small differences between early- and latewood. On the contrary, potassium and sulfur presented lower values in the most stressed plants, and manganese had the lowest values only for moderate water stress. Finally, S/Ca and K/Ca molar ratios decreased, while Ca/Mn increased as stress becomes more severe.

Conclusions
Our findings suggested that A. araucana seedlings invested resources aimed at increasing structural components of the cell wall to prevent cavitation. This would maintain metabolism and cell growth even in unfavorable environmental conditions. Furthermore, the imbalance of manganese and calcium and their consequent ratio (Ca/Mn) could be linked to early adaptive signals to avoid dieback.
Effects of biotic and abiotic factors on ecosystem multifunctionality of plantations
Authors: Tian Jiaxin , Ni Tian , Miao Zheng , Hao Yuanshuo , Ma Aiyun , Dong Lihu , Li Fengri 
Published: June 2024

Abstract
Background
Mixed forests are better than monoculture forests in biodiversity, stand structure and productivity stability. However, a more comprehensive assessment of the ecosystem functions of monoculture and mixed plantations is lacking. We compared the single functions and ecosystem multifunctionality (EMF) in Fraxinus mandshurica and Larix olgensis mixed plantations with monoculture plantations in Northeast China and discussed the influences of biodiversity and environmental factors on EMF.

Results
The mixed plantations had higher biodiversity and ecological functions. Biodiversity was significantly higher in mixed plantations (such as CWMMH, Shrub.Shannon, Shrub.Richness, Herb.Shannon, Herb.Richness), but environmental factors differed less among the three forest types, and belowground diversity differed significantly only in the Bacterial.Shannon and Fungal.Shannon. Mixed plantations showed significant differences in single ecological functions relative to monoculture plantations, with more pronounced differences between mixed plantations and Larix olgensis monoculture plantations. Weighted ecosystem multifunctionality was significantly higher in mixed plantations than in monoculture plantations. EMF was mainly driven by tree diversity, environmental factors, shrub and herb species diversity, and soil microbial alpha diversity, which explained 25.35%, 8.94%, 8.83%, and 7.65% of the variation, respectively.

Conclusions
The establishment of mixed plantations can increase the biodiversity of forest stands and improve the ecosystem functions. These results highlight the advantages of multi-species plantations and the necessity of planting them. They are important for the conservation of biodiversity and the sustainable management of plantations.
The impact of entrepreneurship education on students’ desirability and intentions to pursue an entrepreneurial career: a study in general and vocational secondary schools of Cabo Verde
Authors: Dinis Anabela 
Published: June 2024

AbstractThis study aims to evaluate the impact of the Entrepreneurship Curriculum Programme (ECP) on students' entrepreneurial attitudes, perception of social norms and entrepreneurial intentions. This programme was implemented in upper-secondary level classes in Cabo Verde. The study also assesses if the programme has different impacts in different years of schooling and the mediating role of personal factors (gender and family models), teaching factors (differences between general and vocational education) and contextual factors (different island). The research was conducted on six islands in 4 vocational schools and 8 schools of general studies. The research compared the results of two groups: one with 947 students who attended ECP and the other with 1140 students who did not. The findings reveal that ECP impacts all the dimensions considered, the effect is differentiated throughout schooling and that other factors affect students' entrepreneurial attitudes, beliefs and intentions. From the theoretical point of view, the study reinforces the knowledge about the impact of education in entrepreneurship in contexts where there are still few studies—the context of developing countries and the context of secondary education. From a practical point of view, the study provides indications for deepening/improving the design and implementation of entrepreneurship education programmes.
Hypoxia-mediated programmed cell death is involved in the formation of wooden breast in broilers
Authors: Zhang Xinrui , Xing Tong , Zhang Lin , Zhao Liang , Gao Feng 
Published: June 2024

Abstract
Background
Wooden breast (WB) myopathy is a common myopathy found in commercial broiler chickens worldwide. Histological examination has revealed that WB myopathy is accompanied by damage to the pectoralis major (PM) muscle. However, the underlying mechanisms responsible for the formation of WB in broilers have not been fully elucidated. This study aimed to investigate the potential role of hypoxia-mediated programmed cell death (PCD) in the formation of WB myopathy.

Results
Histological examination and biochemical analysis were performed on the PM muscle of the control (CON) and WB groups. A significantly increased thickness of the breast muscle in the top, middle, and bottom portions (P<0.01) was found along with pathological structure damage of myofibers in the WB group. The number of capillaries per fiber in PM muscle, and the levels of pO2 and sO2 in the blood, were significantly decreased (P < 0.01), while the levels of pCO2 and TCO2 in the blood were significantly increased (P < 0.05), suggesting hypoxic conditions in the PM muscle of the WB group. We further evaluated the PCD-related pathways including autophagy, apoptosis, and necroptosis to understand the consequence response to enhanced hypoxic conditions in the PM muscle of birds with WB. The ratio of LC3 II to LC3 I, and the autophagy-related factors HIF-1α, BNIP3, Beclin1, AMPKα, and ULK1 at the mRNA and protein levels, were all significantly upregulated (P < 0.05), showing that autophagy occurred in the PM muscle of the WB group. The apoptotic index, as well as the expressions of Bax, Cytc, caspase 9, and caspase 3, were significantly increased (P < 0.05), whereas Bcl-2 was significantly decreased (P < 0.05) in the WB-affected PM muscle, indicating the occurrence of apoptosis mediated by the mitochondrial pathway. Additionally, the expressions of necroptosis-related factors RIP1, RIP3, and MLKL, as well as NF-κB and the pro-inflammatory cytokines TNF-α, IL-1β, and IL-6, were all significantly enhanced (P < 0.05) in the WB-affected PM muscle.

Conclusions
The WB myopathy reduces blood supply and induces hypoxia in the PM muscle, which is closely related to the occurrence of PCD including apoptosis, autophagy, and necroptosis within myofibers, and finally leads to abnormal muscle damage and the development of WB in broilers.
Understanding the impact of urban heat islands on crime: insights from temperature, population density, and green canopy cover
Authors: Jonescu Emil E. , Ramanayaka Chamil Erik , Olatunji Oluwole A. , Uylaki Talia J. 
Published: June 2024

AbstractEmergent literature suggests that micro- and macro-climates influence criminal behaviour; a complex phenomenon that is still incipient in theory development. This mixed-method research starts with a systematic review of the literature on the theoretical premises that urban heat islands amplify aggressive behaviour and crime. Further, it discusses the potential implications of the relationship between the environment and social outcomes on the design and planning of urban environments. A meta-synthesis was conducted to explain the correlations between patterns of criminal behaviour and thermal (dis)comfort. This correlation is to relate fundamental urban design principles to socially sustainable communities that dissuade violence and crime, and otherwise show poorly designed spaces do propagate criminal behaviour. Cross-validation was undertaken using a case study of Midland, a suburb of Perth, Western Australia. Data involves population demographics, temperature, and crime statistics relating to Offences against the Person. Analysis focused on homicide, assault, threatening behaviour, and robbery. The findings imply a positive correlation between long-term temperature, crime, population density, and green canopy cover. Variables of climate (e.g., short-to-long-term climate-related stressors) and crime types also show non-linear association. Nonetheless, forecasting the future of violence and trends of crime through attributes or potential impacts of heat and urban canopy cover on the built environment will inform sustainable social development policy, environmental planning, development strategy, designers, and planners. Recommendations are made around these in relation to making urban communities adaptive to the impacts of global warming and future densification.
Facebook addiction and its impact on depression: a cross sectional study
Authors: Salma Nahid , Bhuiyan Farhaduzzaman 
Published: June 2024

Abstract
Aim
Facebook has become a part of daily life for its users and therefore become a very significant factor of mental health. As the number of Facebook users increases exponentially, the problems related to immense use have become more evident and more frequent. Therefore, the study aimed to explore the impact of Facebook addiction on depression among its users.

Methods
An online-based questionnaire was used to collect data from 269 Facebook users of Bangladesh using convenient sampling technique. Bergen Facebook Addiction Scale (BFAS) and Patient Health Questionnaire (PHQ-9) scale was used to assess Facebook addiction and its impact on depression.Ordinal logistic was used to extract the significant variables associated with Facebook addiction and depression.

Results
The result categorized the Facebook users into three categories as : normal (17%), problematic user (52%) and addicted (30.5%). Facebook users were suffering from mild (13.4%), minimal (15.6%), moderate (59.5%), and severe (11.5%) level of depression. Both Facebook addiction and depression were moderately correlated (0.701). Ordinal regression showed, gender $$(\beta$$ (95% CI) = 0.859 (0.223,1.495)), age ($$\beta$$ (95% CI) = -2.051(-3.789, -0.313)), residential area ($$\beta$$ (95% CI) = -0.858(-1.626, -0.09)), occupation ($$\beta \left(95\text{\%} \text{C}\text{I}\right)=-0.494(-\text{1.547,0.559})$$), time length of Facebook use ($$\beta \left(95\text{\%} \text{C}\text{I}\right)=2.288\left(\text{0.324,2.251}\right))$$are the significant predictors of Facebook addiction. Wheras, relationship types and occupation are also responsible for depression.

Conclusion
The study showed large portion of facebook users of Bangladesh are suffering from depression. Authors suggest to arrange mental health campaign to promote bounded and legitimate use of facebook and therefore will accelerate the attaining rate of sustainable development goal related to the Bangladesh’s health status.
Distinctive outlook into the mechanism of resistance to rice blast
Authors: Tan Chao , Wu Jianguo , Men Shuzhen 
Published: June 2024

AbstractRice blast, caused by the filamentous fungus Magnaporthe oryzae (also known as Pyricularia oryzae), is the most severe disease threatening rice yield. Sakuranetin, an important plant phytoalexin, exhibits inhibitory effects on rice blast fungus growth. However, the underlying molecular mechanism by which sakuranetin enhances plant resistance against pathogens remains unclear. A recent study published in Nature Communications by Jiang et al. (Nat. Commun, 2023, https://doi.org/10.1038/s41467-024-47746-y) elucidated from a cytological perspective how sakuranetin enhances resistance to rice blast disease. The research revealed that elevated accumulation of sakuranetin attenuates the endocytosis of M. oryzae effectors into the cytoplasm of rice host cells. The inhibitory effect of sakuranetin is dosage-dependent and targets clathrin-mediated endocytosis (CME). This study provides novel insights into understanding plant immune mechanisms underlying weakening pathogen virulence weapons.
Effects of far-red light on growth, endogenous hormones, antioxidant capacity and quality of Lettuce
Authors: Meng Lili , Song Jiangfeng , Ni Degang , Kamaruddin Mohamad Anuar 
Published: June 2024

AbstractThis study aimed to explore the effects of various intensities of far-red light on the growth performance, endogenous hormones, antioxidant indices, and overall quality of hydroponically cultivated lettuce. As the control treatment, a white LED emitting light at an intensity of 200 µmol/(m2·s) was utilized (referred to as CK with an R/FR ratio of 5.5), while two experimental treatments, FT1 (R/FR = 1.2) and FT2 (R/FR = 0.8), were established by adding different intensities of far-red light to the CK treatment. The results demonstrated that the application of far-red light, particularly in FT1, led to a significant increase in plant height, leaf area, and lettuce biomass, while simultaneously resulting in a notable reduction in leaf thickness. The content of indole-3-acetic acid (IAA) and abscisic acid (ABA) in response to far-red light treatments exhibited an initial increase followed by a subsequent decrease, with FT2 experiencing a significant decline. The gibberellin (GA3) content in FT2 reached its peak on the 35th day, showing a substantial increase of 60.09% compared to CK. Far-red treatments were found to enhance peroxidase (POD) and catalase (CAT) activities, while significantly reducing superoxide dismutase (SOD) activity. In comparison to CK, FT1 exhibited a remarkable 134.33% increase in anthocyanin content. Both FT1 and FT2 significantly boosted vitamin C levels while reducing nitrite content. Additionally, the application of far-red light treatment significantly increased the alcohol and ester content in lettuce leaves. This study establishes a theoretical foundation for enhancing the quality and flavor of lettuce using different far-red light treatments.
Graphical Abstract