bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum peptidome patterns of breast cancer based on magnetic bead separation and mass spectrometry analysis
Authors: Fan Nai-Jun , Gao Chun-Fang , Zhao Guang , Wang Xiu-Li , Liu Qing-Yin 
Published: April 2012

Abstract

Background
Breast cancer is one of the most common cancers in the world, and the identification of biomarkers for the early detection of breast cancer is a relevant target. The present study aims to determine serum peptidome patterns for screening of breast cancer.

Methods
The present work focused on the serum proteomic analysis of 36 healthy volunteers and 37 breast cancer patients using a ClinProt Kit combined with mass spectrometry (MS). This approach allows the determination of peptidome patterns that are able to differentiate the studied populations. An independent group of sera (36 healthy volunteers and 37 breast cancer patients) was used to verify the diagnostic capabilities of the peptidome patterns blindly. An immunoassay method was used to determine the serum mucin 1 (CA15-3) of validation group samples.

Results

S upport Vector Machine (SVM) Algorithm was used to construct the peptidome patterns for the identification of breast cancer from the healthy volunteers. Three of the identified peaks at m/z 698, 720 and 1866 were used to construct the peptidome patterns with 91.78% accuracy. Furthermore, the peptidome patterns could differentiate the validation group achieving a sensitivity of 91.89% (34/37) and a specitivity of 91.67% (33/36) (> CA 15–3, P < 0.05).

Conclusions
These results suggest that the ClinProt Kit combined with MS shows great potentiality for the diagnosis of breast cancer.

Virtual slides
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1501556838687844
Is there a link between diabetic glomerular injury and crescent formation? A case report and literature review
Authors: Otani Naoko , Akimoto Tetsu , Yumura Wako , Matsubara Daisuke , Iwazu Yoshitaka , Numata Akihiko , Miki Takuya , Takemoto Fumi , Fukushima Noriyoshi , Muto Shigeaki , Kusano Eiji 
Published: April 2012

Abstract
Glomerular crescents are most commonly associated with rapidly progressive crescentic glomerulonephritis; however, they also develop in response to a wide range of primary and secondary glomerular injuries. Since various kind of glomerulopathies occasionally overlay diabetic glomerular injuries, the presence of crescents in renal biopsy specimens of diabetics may have stimulated a search for etiologies other than diabetes. In this report, we describe an unusual case of diabetic glomerulosclerosis with peculiar extracapillary proliferation. Although such a relationship has so far been ignored in most of the literature, the etiological linkage between diabetic glomerulosclerosis and the development of crescents may not be exceptional. We have reviewed the previous literature and herein discuss the pathological implications of the development of crescents in patients with diabetic glomerulosclerosis.

Virtual slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/3950457896920255.
Factors influencing heterogeneity of radiation-induced DNA-damage measured by the alkaline comet assay
Authors: Seidel Clemens , Lautenschläger Christine , Dunst Jürgen , Müller Arndt-Christian 
Published: April 2012

Abstract

Background
To investigate whether different conditions of DNA structure and radiation treatment could modify heterogeneity of response. Additionally to study variance as a potential parameter of heterogeneity for radiosensitivity testing.

Methods
Two-hundred leukocytes per sample of healthy donors were split into four groups. I: Intact chromatin structure; II: Nucleoids of histone-depleted DNA; III: Nucleoids of histone-depleted DNA with 90 mM DMSO as antioxidant. Response to single (I-III) and twice (IV) irradiation with 4 Gy and repair kinetics were evaluated using %Tail-DNA. Heterogeneity of DNA damage was determined by calculation of variance of DNA-damage (V) and mean variance (Mvar), mutual comparisons were done by one-way analysis of variance (ANOVA).

Results
Heterogeneity of initial DNA-damage (I, 0 min repair) increased without histones (II). Absence of histones was balanced by addition of antioxidants (III). Repair reduced heterogeneity of all samples (with and without irradiation). However double irradiation plus repair led to a higher level of heterogeneity distinguishable from single irradiation and repair in intact cells. Increase of mean DNA damage was associated with a similarly elevated variance of DNA damage (r = +0.88).

Conclusions
Heterogeneity of DNA-damage can be modified by histone level, antioxidant concentration, repair and radiation dose and was positively correlated with DNA damage. Experimental conditions might be optimized by reducing scatter of comet assay data by repair and antioxidants, potentially allowing better discrimination of small differences. Amount of heterogeneity measured by variance might be an additional useful parameter to characterize radiosensitivity.
Deficiency of the p53/p63 target Perp alters mammary gland homeostasis and promotes cancer
Authors: Dusek Rachel L , Bascom Jamie L , Vogel Hannes , Baron Sylvain , Borowsky Alexander D , Bissell Mina J , Attardi Laura D 
Published: April 2012

Abstract
Introduction
Perp is a transcriptional target of both p53 during DNA damage-induced apoptosis and p63 during stratified epithelial development. Perp-/- mice exhibit postnatal lethality associated with dramatic blistering of the epidermis and oral mucosa, reflecting a critical role in desmosome-mediated intercellular adhesion in keratinocytes. However, the role of Perp in tissue homeostasis in other p63-dependent stratified epithelial tissues is poorly understood. Given that p63 is essential for proper mammary gland development and that cell adhesion is fundamental for ensuring the proper architecture and function of the mammary epithelium, here we investigate Perp function in the mammary gland.

Methods
Immunofluorescence and Western blot analysis were performed to characterize Perp expression and localization in the mouse mammary epithelium throughout development. The consequences of Perp deficiency for mammary epithelial development and homeostasis were examined by using in vivo mammary transplant assays. Perp protein levels in a variety of human breast cancer cell lines were compared with those in untransformed cells with Western blot analysis. The role of Perp in mouse mammary tumorigenesis was investigated by aging cohorts of K14-Cre/+;p53
fl/fl
mice that were wild-type or deficient for Perp. Mammary tumor latency was analyzed, and tumor-free survival was assessed using Kaplan-Meier analysis.

Results
We show that Perp protein is expressed in the mammary epithelium, where it colocalizes with desmosomes. Interestingly, although altering desmosomes through genetic inactivation of Perp does not dramatically impair mammary gland ductal development, Perp loss affects mammary epithelial homeostasis by causing the accumulation of inflammatory cells around mature mammary epithelium. Moreover, we show reduced Perp expression in many human breast cancer cell lines compared with untransformed cells. Importantly, Perp deficiency also promotes the development of mouse mammary cancer.

Conclusions
Together, these observations demonstrate an important role for Perp in normal mammary tissue function and in mammary cancer suppression. In addition, our findings highlight the importance of desmosomes in cancer suppression and suggest the merit of evaluating Perp as a potential prognostic indicator or molecular target in breast cancer therapy.
Identification of gas hydrate dissociation from wireline-log data in the Shenhu area, South China Sea
Authors: Wang Xiujuan , Lee Myung , Wu Shiguo , Yang Shengxiong 
Published: April 2012

Wireline logs were acquired in eight wells during China’s first gas hydrate drilling expedition (GMGS-1) in April–June of 2007. Well logs obtained from site SH3 indicated gas hydrate was present in the depth range of 195–206 m below seafloor with a maximum pore-space gas hydrate saturation, calculated from pore water freshening, of about 26%. Assuming gas hydrate is uniformly distributed in the sediments, resistivity calculations using Archie’s equation yielded hydrate-saturation trends similar to those from chloride concentrations. However, the measured compressional (P-wave) velocities decreased sharply at the depth between 194 and 199 mbsf, dropping as low as [Formula: see text], indicating the presence of free gas in the pore space, possibly caused by the dissociation of gas hydrate during drilling. Because surface seismic data acquired prior to drilling were not influenced by the in situ gas hydrate dissociation, surface seismic data could be used to identify the cause of the low P-wave velocity observed in the well log. To determine whether the low well-log P-wave velocity was caused by in situ free gas or by gas hydrate dissociation, synthetic seismograms were generated using the measured well-log P-wave velocity along with velocities calculated assuming both gas hydrate and free gas in the pore space. Comparing the surface seismic data with various synthetic seismograms suggested that low P-wave velocities were likely caused by the dissociation of in situ gas hydrate during drilling.
TLX controls angiogenesis through interaction with the von Hippel-Lindau protein
Authors: Zeng Zhao-jun , Johansson Erik , Hayashi Amiko , Chavali Pavithra L. , Akrap Nina , Yoshida Takeshi , Kohno Kimitoshi , Izumi Hiroto , Funa Keiko 
Published: April 2012

Summary
TLX is known as the orphan nuclear receptor indispensable for maintaining neural stem cells in adult neurogenesis. We report here that neuroblastoma cell lines express high levels of TLX, which further increase in hypoxia to enhance the angiogenic capacity of these cells. The proangiogenetic activity of TLX appears to be induced by its direct binding to the von Hippel-Lindau protein (pVHL), which stabilizes TLX. In turn, TLX competes with hydroxylated hypoxia-inducible factor (HIF-α) for binding to pVHL, which contributes to the stabilization of HIF-2α in neuroblastoma during normoxia. Upon hypoxia, TLX increases in the nucleus where it binds in close proximity of the HIF-response element on the VEGF-promoter chromatin, and, together with HIF-2α, recruits RNA polymerase II to induce VEGF expression. Conversely, depletion of TLX by shRNA decreases the expression of HIF-2α and VEGF as well as the growth-promoting and colony-forming capacity of the neuroblastoma cell lines IMR-32 and SH-SY5Y. On the contrary, silencing HIF-2α will slightly increase TLX, suggesting that TLX acts to maintain a hypoxic environment when HIF-2α is decreasing. Our results demonstrate TLX to play a key role in controlling angiogenesis by regulating HIF-2α. TLX and pVHL might counterbalance each other in important fate decisions such as self-renewal and differentiation, as well as angiogenesis and anti-angiogenesis.
B Cells With High Side Scatter Parameter by Flow Cytometry Correlate With Inferior Survival in Diffuse Large B-Cell Lymphoma
Authors: Bashashati Ali , Johnson Nathalie A. , Khodabakhshi Alireza Hadj , Whiteside Matthew D. , Zare Habil , Scott David W. , Lo Kenneth , Gottardo Raphael , Brinkman Fiona S.L. , Connors Joseph M. , Slack Graham W. , Gascoyne Randy D. , Weng Andrew P. , Brinkman Ryan R. 
Published: April 2012

AbstractDespite advances in the understanding of diffuse large B-cell lymphoma (DLBCL) biology, only the clinically based International Prognostic Index (IPI) is used routinely for risk stratification at diagnosis. To find novel prognostic markers, we analyzed flow cytometric data from 229 diagnostic DLBCL samples using an automated multiparameter data analysis approach developed in our laboratory. By using the developed automated data analysis pipeline, we identified 71 of 229 cases as having more than 35% B cells with a high side scatter (SSC) profile, a parameter reflecting internal cellular complexity. This high SSC B-cell feature was associated with inferior overall and progression-free survival (P = .001 and P = .01, respectively) and remained a significant predictor of overall survival in multivariate Cox regression analysis (IPI, P = .001; high SSC, P = .004; rituximab, P = .53).This study suggests that high SSC among B cells may serve as a useful biomarker to identify patients with DLBCL at high risk for relapse. This is of particular interest because this biomarker is readily available in most clinical laboratories without significant alteration to existing routine diagnostic strategies or incurring additional costs.
Copper Corrosion in Aqueous Sulfide Solutions under Nuclear Waste Repository Conditions
Authors: Chen J. , Qin Z. , Shoesmith D. W. 
Published: April 2012

ABSTRACTThe corrosion behavior of oxygen-free copper in anoxic sulfide solutions under nuclear waste disposal conditions was studied using electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM) equipped with a focused ion beam (FIB), X-ray photoelectron spectroscopy (XPS) and Micro X-ray diffraction (μXRD). The film growth process and mechanism were elucidated using an Au marker test, and the contribution of solution diffusion to corrosion was demonstrated in magnetically-stirred experiments. The effect of groundwater chemistry, particularly chloride content on copper corrosion and film properties was characterized using long-term corrosion experiments.
Separation of alicyclic and aromatic hydrocarbons on a PLOT column coated with 3-benzylketoiminepropyl group
Authors: Wawrzyniak Rafał 
Published: April 2012

AbstractAn interpretation of specific electron-donor-acceptor interactions between the adsorbent coating the walls of a capillary column and adsorbates from the groups of alicyclic and aromatic hydrocarbons is proposed. The adsorbent was based on silica the surface of which was modified with silane containing 3-benzylketoimine groups to improve its adsorption properties. The columns studied had walls coated with 3-benzylketoimine groups, and Cu(II) chloride complexes or with Ni(II) chloride complexes. The adsorbate-adsorbent interactions were interpreted on the basis of the Kovats retention index, specific retention volume, molecular retention index, and ΔM e values. The influence of particular elements of spatial structure and the positions of double bonds in the adsorbate molecule was evaluated on the modelling calculations based on the quantitative structure-retention relationships. The introduction of transition metal ions in the form of complexes into the adsorbents studied increased the strength of the interactions between the adsorption layer of the capillary column and the adsorbate molecules. The increased strength of the interactions was accompanied by increased selectivity of the columns with regard to a group of alicyclic and aromatic hydrocarbons. The analytical performance of the columns thus obtained was compared with that of a commercial column coated with the 100 % dimethyl polysiloxane phase.
Influence of purine on copper behavior in neutral and alkaline sulfate solutions
Authors: Petrović Marija , Simonović Ana , Radovanović Milan , Milić Snežana , Antonijević Milan 
Published: April 2012

AbstractThe effect of purine (concentration range of 1.00 × 10−6–1.00 × 10−2 M) on the behavior of copper in a 0.5 M Na2SO4 solution (pH 7 and pH 9) was studied using the open circuit potential measurement, potentiodynamic polarization, and chronoamperometry. Potentiodynamic polarization shows that purine acts as a copper corrosion inhibitor in both alkaline and neutral sulfate solutions. The efficiency of inhibition increases as the purine concentration increases. Chronoamperometric results follow the same trend as the results of potentiodynamic polarization. The inhibition effect can also be observed visually by microscopic examination of the electrode surface. Purine is adsorbed on copper surface according to the Langmuir adsorption isotherm.
Development of polyaniline microarray electrodes for cadmium analysis
Authors: Oliveira Marta , Viswanathan Subramanian , Morais Simone , Delerue-Matos Cristina 
Published: April 2012

AbstractDisposable screen-printed electrodes (SPCE) were modified using a cosmetic product to partially block the electrode surface in order to obtain a microelectrode array. The microarrays formed were electropolymerized with aniline. Scanning electron microscopy was used to evaluate the modified and polymerized electrode surface. Electrochemical characteristics of the constructed sensor for cadmium analysis were evaluated by cyclic and square-wave voltammetry. Optimized stripping procedure in which the preconcentration of cadmium was achieved by depositing at −1.20 V (vs. Ag/AgCl) resulted in a well defined anodic peak at approximately −0.7 V at pH 4.6. The achieved limit of detection was 4 × 10−9 mol dm−3. Spray modified and polymerized microarray electrodes were successfully applied to quantify cadmium in fish sample digests.
A morphological study of Durance River terraces from Tallard to Avignon, South-East France
Authors: Warner Robin 
Published: April 2012

Abstract
This study of Durance River terrace morphologies in the lower 200 km of the valley shows that terrace ages, elevations and heights above the river generally all increase upstream. It is based on extensive fieldwork checked against geological maps and memoirs. Subdivision of the valley, based on gorges and former tectonic blockages, is relevant because there are no Durance terraces downstream of Mallemort and there are considerable differences between each reach. For most of the Pleistocene, the river was diverted through the Crau Plains to the sea. Below the Mirabeau gorge, there are a few narrow low and middle terraces. The best terrace development is between Mirabeau and the Sisteron defile. Upstream of that barrier there is glacial dominance. Changes in terrace continuity are illustrated by means of height-range diagrams and their rates of dissection have been quantified approximately, based on cosmic ray exposure age modelling. Climate forcing cannot explain all the changes found. The influence of tectonic activity was found to increase in an upstream direction and also with age on the southwestern flanks of the Alps.
Wardium Ponticum Sp. N. (Cestoda, Cyclophyllidea, Hymenolepidoidea), a Parasite of Pratincole (Glareola Pratincola) from the Black Sea Coast
Authors: Kornyushin V. , Georgiev B. , Greben O. 
Published: April 2012

Wardium PonticumSp. N. (Cestoda, Cyclophyllidea, Hymenolepidoidea), a Parasite of Pratincole (Glareola Pratincola) from the Black Sea CoastThe new speciesWardium ponticumKornyushin, Georgiev et Greben, sp. n. (Aploparksidae Mayhew, 1925) parasitic in pratincole (Glareola pratincolaLinnaeus, 1766) from Bulgaria and Ukraine is described. The species is characterized by 10 aploparaksoid hooks, 9-10 mm long, and clearly differs from all congeneric species by the shape and armament of the cirrus.
A New Species of Baccharomyia (Diptera, Cecidomyiidae) from Baccharis Pseudomiryocephala (Asteraceae) in Brazil
Authors: Maia V. , Carneiro M. 
Published: April 2012

A New Species of Baccharomyia (Diptera, Cecidomyiidae) from Baccharis Pseudomiryocephala (Asteraceae) in Brazil
Baccharomyia magna Maia, sp. n., a gall midge (Lasiopteridi) that induces galls on Baccharis pseudomiryocephala (Asteraceae) is described and illustrated based on material from Minas Gerais, Brazil. A key to species is presented.
Morphological Characteristics of Male Genitalia in Some Species in the Genus Torymus
Authors: Klymenko S. 
Published: April 2012

Morphological Characteristics of Male Genitalia in Some Species in the GenusTorymusMorphology of male genitalia in some species in the genusTorymusDalman is studied for the first time. In contrast to genitalia of Eurytomidae species, where this structure is highly species-specific, the structure of genitalia in Torymidae species show no distinct specific differences: phallobase in studied species is opened; most species have three hooks on digital sclerites with the small indents at their bases.
Subject-subject relationship as a significant aspect of personal development in adulthood
Authors: Zagórska Wanda 
Published: April 2012

Subject-subject relationship as a significant aspect of personal development in adulthood
The issue of the subject-subject relationship, also known as the relationship of encounter or the I-Thou relationship, which has a strong presence in the humanities and Christian mysticism, is rarely addressed by psychology. This type of relationship goes beyond the psychosocial approach to personal maturity and human development at the so-called higher stages, thus falling outside the predominant lines of psychological inquiry. Consequently, this paper concerns issues that are not popular in psychology, albeit they are close to the problem of the person's development as a subject. Drawing inspiration from cultural anthropology and intersubjective philosophy - especially Martin Buber's philosophy of dialogue and Gabriel Marcel's concrete philosophy - the author presents from a psychological stance the phenomenon of the subject-subject relationship and its prerequisite conditions. Adopting the perspective of personalistic psychology in its existentialist-phenomenological strands, the author indicates the place of this relationship in human personal development and highlights its crucial significance to this process.
Integration of Schwartz's value theory and Scheler's concept of value in research on the development of the structure of values during adolescence
Authors: Cieciuch Jan 
Published: April 2012

Integration of Schwartz's value theory and Scheler's concept of value in research on the development of the structure of values during adolescence
A proposal is presented in the article of integrating Schwartz's circular model of values (1992, 1994, 2006) with Scheler's concept of values (Brzozowski, 1995). The main research goals were: 1) empirical verification of the attempt to include the values of Scheler into the circle of Schwartz's values; 2) use of the concept and measurement of Scheler's values to describe the development of the value structure during adolescence. Two studies were conducted in a group of 988 persons aged from 15 to 20 years. The Scheler Value Scale of Brzozowski (1995) was used along with the new version of the Schwartz's Portrait Value Questionnaire (Schwartz et al., 2011). In the first study, multidimensional scaling of multitrait-multimathod (MTMM) matrix was carried out, into which were introduced the indexes of Scheler's values and the indexes of Schwartz's value types. In this way, it was demonstrated that it is possible to include Scheler's values in the four higher order values within Schwartz's circle of values. In the second study, it was shown that by using the Schelerian values to analyse the development of the value structure, similar results were obtained to those that were acquired with the aid of instruments intended to measure values in Schwartz's approach. The structure becomes differentiated with age and takes on the shape of a circle.