bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serum levels of IGF‐I, IGFBP‐3 and colorectal cancer risk: results from the EPIC cohort, plus a meta‐analysis of prospective studies
Authors: Rinaldi Sabina , Cleveland Rebecca , Norat Teresa , Biessy Carine , Rohrmann Sabine , Linseisen Jakob , Boeing Heiner , Pischon Tobias , Panico Salvatore , Agnoli Claudia , Palli Domenico , Tumino Rosario , Vineis Paolo , Peeters Petra H.M. , van Gils Carla H. , Bueno‐de‐Mesquita Bas H. , Vrieling Alina , Allen Naomi E. , Roddam Andrew , Bingham Sheila , Khaw Kay‐Tee , Manjer Jonas , Borgquist Signe , Dumeaux Vanessa , Torhild Gram Inger , Lund Eiliv , Trichopoulou Antonia , Makrygiannis Georgios , Benetou Vassiliki , Molina Esther , Donate Suárez Ignacio , Barricarte Gurrea Aurelio , Gonzalez Carlos A. , Tormo Maria‐Jose , Altzibar Jone M. , Olsen Anja , Tjonneland Anne , Grønbæk Henning , Overvad Kim , Clavel‐Chapelon Françoise , Boutron‐Ruault Marie‐Christine , Morois Sophie , Slimani Nadia , Boffetta Paolo , Jenab Mazda , Riboli Elio , Kaaks Rudolf 
Published: October 2009

AbstractSeveral prospective studies have shown a moderate positive association between increasing circulating insulin‐like growth factor‐I (IGF‐I) levels and colorectal cancer risk. However, the associations were often statistically nonsignificant, and the relationship of cancer risk with IGF‐I's major binding protein, IGFBP‐3, showed major discrepancies between studies. We investigated the association of colorectal cancer risk with serum IGF‐I, total and intact IGFBP‐3, in a case‐control study nested within the EPIC cohort (1,121 cases of colorectal cancer and 1,121 matched controls). Conditional logistic regression was used to adjust for possible confounders. Our present study results were combined in a meta‐analysis with those from 9 previous prospective studies to examine the overall evidence for a relationship of prediagnostic serum IGF‐I with colorectal cancer risk. In the EPIC study, serum concentrations of IGF‐I and IGFBP‐3 showed no associations with risk of colorectal cancer overall. Only in subgroup analyses did our study show moderate positive associations of IGF‐I levels with risk, either among younger participants only (and only for colon cancer) or among participants whose milk intakes were in the lowest tertile of the population distribution (RR for an increase of 100 ng/ml = 1.43 [95% CI = 1.13–1.93]). Nevertheless, in the meta‐analysis a modest positive association remained between serum IGF‐I and colorectal cancer risk overall (RR = 1.07 [1.01–1.14] for 1 standard deviation increase in IGF‐I). Overall, data from our present study and previous prospective studies combined indicate a relatively modest association of colorectal cancer risk with serum IGF‐I.
Combination of p53 codon 72 polymorphism and inactive p53 mutation predicts chemosensitivity to 5‐fluorouracil in colorectal cancer
Authors: Tominaga Toshiji , Iwahashi Makoto , Takifuji Katsunari , Hotta Tsukasa , Yokoyama Shozo , Matsuda Kenji , Higashiguchi Takashi , Oku Yoshimasa , Nasu Toru , Yamaue Hiroki 
Published: October 2009

AbstractThere are increasing reports showing the clinical significance of the p53 polymorphism status in terms of the response to chemotherapy. We investigated whether p53 polymorphism and mutation were associated with in vitro sensitivity to 5‐fluorouracil (5‐FU) in patients with colorectal cancer. Chemosensitivity to 5‐FU was evaluated by the collagen gel droplet embedded culture drug sensitivity test. 5‐FU sensitivity of tumor cells without inactive p53 mutation in the arginine/arginine (Arg/Arg) variant was significantly higher than that of tumor cells with or without inactive p53 mutation in other variants (p = 0.022), whereas the 5‐FU sensitivity of tumor cells with inactive p53 mutation in the Arg/Arg variant was significantly lower than that of tumor cells with or without inactive p53 mutation in other variants (p = 0.002). In the Arg/Arg variant, apoptotic cells induced by 5‐FU treatment in patients without inactive p53 mutation were more markedly increased than those in patients with inactive p53 mutation (p = 0.037). Bax and Bcl‐2 protein expressions in tumor tissue treated with 5‐FU were associated with both 5‐FU sensitivity and the apoptotic cell count. Our data show that the Arg/Arg genotype without inactive p53 mutation could be predictive of a more favorable response and the Arg/Arg genotype with inactive p53 mutation a less favorable response to chemotherapy using 5‐FU in CRC. The combination of the p53 codon 72 polymorphism and p53 mutation status is a potential predictive marker of sensitivity to 5‐FU in CRC.
Skin cancers associated with HIV infection and solid‐organ transplantation among elderly adults
Authors: Lanoy Emilie , Costagliola Dominique , Engels Eric A. 
Published: October 2009

AbstractImmunosuppression may be etiologic for some skin cancers. We investigated the impact of human immunodeficiency virus (HIV) infection and solid‐organ transplantation on skin cancer risk. We conducted a population‐based case–control study among elderly U.S. adults (non‐Hispanic whites, age 67 years or older), using Surveillance, Epidemiology and End Results Medicare linked data. The study comprised 29,926 skin cancer cases (excluding basal cell and squamous cell carcinomas) and 119,704 controls, frequency‐matched by gender, age and calendar year (1987–2002). Medicare claims identified solid‐organ transplantation or HIV infection before cancer diagnosis/control selection. As negative controls, we evaluated other medical conditions (e.g., hypertension and depression) and cancers (breast, colon and prostate) not linked to immunosuppression. Odds ratios (ORs) compared prevalence in cases and controls, adjusted for matching factors and number of prior physician claims. Risks of Kaposi sarcoma (N = 602) and cutaneous non‐Hodgkin lymphoma (N = 1,836) were increased with solid‐organ transplantation (OR [95%CI]: 11.06 [5.27–23.23] and 2.27 [1.00–5.15], respectively) and HIV infection (21.58 [11.94–38.99] and 2.41 [1.05–5.52], respectively). Solid‐organ transplantation was also associated with increased risks of Merkel cell carcinoma (N = 1,286; OR [95%CI] 4.95 [2.62–9.34]) and other cutaneous sarcomas (N = 972; 4.19 [1.83–9.56]). Solid‐organ transplantation was nonsignificantly associated with melanoma (N = 23,974; (OR 1.36 [95%CI 0.98–1.88]). Null or weak associations were observed for negative control medical conditions and cancers. Solid‐organ transplantation and HIV infection were followed by increased risk for some skin cancer subtypes among elderly adults. These results highlight the potential role of immunity in development of skin cancers.
Sphingosine‐1‐phosphate receptor type 1 regulates glioma cell proliferation and correlates with patient survival
Authors: Yoshida Yuya , Nakada Mitsutoshi , Sugimoto Naotoshi , Harada Tomoya , Hayashi Yasuhiko , Kita Daisuke , Uchiyama Naoyuki , Hayashi Yutaka , Yachie Akihiro , Takuwa Yoh , Hamada Jun‐ichiro 
Published: October 2009

AbstractSphingosine‐1‐phosphate (S1P) is a bioactive lipid that signals through a family of G protein‐coupled receptors consisting of 5 members termed S1P1–5, and it regulates cellular proliferation, migration and survival. We investigated the expression and role of S1P receptors in glioma. Human glioma expressed S1P1, S1P2, S1P3, and S1P5 by quantitative real‐time PCR analysis. Expression of the S1P1 was significantly lower in glioblastoma than in the normal brain (p < 0.01) and diffuse astrocytoma (p < 0.05). Immunoblotting showed that normal brain expressed more S1P1 protein than did glioblastoma. Immunohistochemistry showed that S1P1 was localized predominantly in the astrocytes in the normal brain, but no staining was observed in glioblastoma. Downregulation of S1P1 expression correlated with poor survival of patients with glioblastoma (p < 0.05). S1P1 small interfering RNA promoted cell proliferation in high‐expressor glioma cell lines (T98G, G112). Cell proliferation was promoted by the pertussis toxin, which deactivates Gi/o type of G proteins; the S1P1 is exclusively coupled to these proteins. Forced expression of the S1P1 in low‐expressor cell lines (U87, U251) resulted in decreased cell growth and led to suppressed tumor growth in transplanted gliomas in vivo. Furthermore, we found a significant association between the S1P1 expression and early growth response‐1, a transcriptional factor that exhibits tumor suppression in glioblastoma cells (p < 0.05). These data indicate that the downregulation of S1P1 expression enhances the malignancy of glioblastoma by increasing cell proliferation and correlates with the shorter survival of patients with glioblastoma.
Selective laser melting: A unit cell approach for the manufacture of porous, titanium, bone in‐growth constructs, suitable for orthopedic applications. II. Randomized structures
Authors: Mullen Lewis , Stamp Robin C. , Fox Peter , Jones Eric , Ngo Chau , Sutcliffe Christopher J. 
Published: October 2009

AbstractIn this study, the unit cell approach, which has previously been demonstrated as a method of manufacturing porous components suitable for use as orthopedic implants, has been further developed to include randomized structures. These random structures may aid the bone in‐growth process because of their similarity in appearance to trabecular bone and are shown to carry legacy properties that can be related back to the original unit cell on which they are ultimately based. In addition to this, it has been shown that randomization improves the mechanical properties of regular unit cell structures, resulting in anticipated improvements to both implant functionality and longevity. The study also evaluates the effect that a post process sinter cycle has on the components, outlines the improved mechanical properties that are attainable, and also the changes in both the macro and microstructure that occur. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2010
Inflammatory pseudotumor (myoblastic tumor) of the breast: A case report and review of the literature
Authors: Park Sung Bin , Kim Hak Hee , Shin Hee Jung , Gong Gyungyub 
Published: October 2009

AbstractInflammatory pseudotumor of the breast is a very rare cause of breast mass. To our knowledge, only a few cases have been described in the English literature. In this case, the lesion appeared on mammography as a round high‐density mass with ill‐defined margins and on sonography as an irregular mass of complex echogenicity with ill‐defined margins and an echogenic rim. The lesion was resected with no evidence of local recurrence after 3 years. © 2009 Wiley Periodicals, Inc. J Clin Ultrasound 2010
Continuous‐type splenogonadal fusion associated with an ipsilateral testicular atrophy: Sonographic findings
Authors: Keyik Bahri , Yanik Bahar , Conkbayir IŞik , Tuygun Can , Kizilgoz Volkan , HekimoĞlu Baki 
Published: October 2009

AbstractSplenogonadal fusion is a rare congenital anomaly in which the spleen is abnormally connected to the gonad. Two types of splenogonadal fusion have been described: continuous and discontinuous. Splenogonadal fusion is frequently associated with cryptorchidism and/or congenital orofacial/limb anomalies. We describe the ultrasound findings in a case of continuous‐type splenogonadal fusion associated with ipsilateral testicular atrophy with correlation with MRI. © 2009 Wiley Periodicals, Inc. J Clin Ultrasound, 2010
Genotypic characterization of non starter lactic acid bacteria involved in the ripening of artisanal Bitto PDO cheese
Authors: Colombo Francesca , Borgo Francesca , Fortina Maria Grazia 
Published: October 2009

AbstractBitto of Valchiavenna, an artisanal Italian cheese produced without the addition of any starter cultures, has been attributed a protected designation origin (PDO) cheese, but the strain composition of the natural microbial population colonizing this traditional dairy product is still unknown. To obtain preliminary information on the non starter lactic acid bacteria involved in its ripening, a total of 136 NSLAB isolates, randomly selected from MRS and M17 agar plates, were collected from three different cheese samples after 120 days of ripening. The new isolates were identified by combining PCR 16S–23S rDNA spacer analyses, partial 16S rRNA gene sequencing, species‐specific probes and colony hybridization. Eighty‐two isolates, representing 60% of the total strains selected, were homofermentative cocci: 83% of them were enterococci, with Enterococcus durans being the predominant species found. Pediococcus spp. were also isolated, together with strains of Streptococcus thermophilus. Within lactobacilli, 57% of the isolates were identified as Lactobacillus paracasei; Lact. curvatus, Lact. plantarum, Lact. fermentum, were present in a lower amount. The isolates were differentiated at strain‐level by Rep‐PCR analysis. This is the first effort to microbiological characterization of Valchiavenna's Bitto; the results suggest the possibility of preserving the wild bacterial population in order to protect the typical organoleptic characteristics of this traditional raw milk cheese and to select new strains for the dairy industry. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Co‐production of γ ‐glutamylcysteine and glutathione by mutant strain Saccharomyces cerevisiae FC‐3 and its kinetic analysis
Authors: Chen When‐Chang , Huang Fu‐Kuo , Cheng Shu‐Chuan , Tsai Feng‐Yuan , Lin Chung‐Liang 
Published: October 2009

AbstractCo‐production of γ ‐glutamylcysteine (γ ‐GC) and glutathione (GSH) by a novel mutant strain Saccharomyces cerevisiae FC‐3 and its kinetic analysis were investigated. The strain could produce γ ‐GC and GSH with high yields (4.22 and 14.3 mg/g‐DCW, respectively) in batch submerged cultures. Effects of medium components and cultivation conditions on cell growth and the contents of intracellular γ ‐GC and GSH were examined. Results show that 2% (w/v) sucrose and 2.5% (w/v) yeast extract are the best carbon and nitrogen sources, respectively, and supplement of three amino acids (glycine, cysteine and glutamate), each at 0.08% (w/v), in the medium could enhance γ ‐GC and GSH production. In addition, optimal operating conditions are at the initial pH value of 7.0, 30 °C and 200 rev/min. Moreover, results obtained from kinetic analyses reveal that γ ‐GC production is mixed‐type growth associated, but GSH production is growth‐associated. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Characterization of new biosurfactant produced by Trichosporon montevideense CLOA 72 isolated from dairy industry effluents
Authors: Monteiro Andrea S. , Coutinho Joana O. P. A. , Júnior Ary C. , Rosa Carlos A. , Siqueira Ezequias P. , Santos Vera L. 
Published: October 2009

AbstractThe yeast strain CLOA 72 isolated from the effluent of a dairy industry in Brazil and identified as Trichosporon montevideense, was able to grow and produce a glycolipid biosurfactant when cultured on a mineral medium (MM) with sunflower oil as the carbon source. Biosurfactant production was partially growth‐associated and maximal emulsification activity was observed at 144 h of cultivation (78.92%). The biosurfactant purified by precipitation with ethanol showed 78.66% emulsifying activity when used in concentrations above 4.5 mg/ml and was able to reduce the surface tension of water to values below 44.9 mN/m. The critical micellar concentration (CMC) was found to be 2.2 mg/ml. The highest emulsifying activity (E24) has been observed with vegetable oils, toluene, kerosene, isooctane, cyclohexane, hexane, diesel oil and hexadecane as compared to mineral oil and oleic acid. The biosurfactant also showed good stability during exposure to 100 °C for different periods of time (10 to 60 min), to high salinity (30% of NaCl, KCl and NaHCO3), and to a wide range of pH values (1–10). The biosurfactant purified by gel filtration chromatography is a glycolipid, with lipid portion containing 16.03% (9Z)‐octadec‐9‐enoic acid, 14.92% hexadecanoic acid, and 9.63% (E) octadec‐9‐enoic acid and the carbohydrate portion containing mannose (35.29%), xylose (41.99%), arabinose (17.47%), and glucose (5.25%). (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Low‐intensity pulsed ultrasound (LIPUS) increases the articular cartilage type II collagen in a rat osteoarthritis model
Authors: Naito Kiyohito , Watari Taiji , Muta Tomoya , Furuhata Atsushi , Iwase Hideaki , Igarashi Mamoru , Kurosawa Hisashi , Nagaoka Isao , Kaneko Kazuo 
Published: October 2009

AbstractIn this study, the effect of low‐intensity pulsed ultrasound (LIPUS) on cartilage was evaluated in a rat osteoarthritis (OA) model using serum biomarkers such as CTX‐II (type II collagen degradation) and CPII (type II collagen synthesis) as well as histological criteria (Mankin score and immunohistochemical type II collagen staining). OA was surgically induced in the knee joint of rats by anterior cruciate/medial collateral ligament transection and medial meniscus resection (ACLT + MMx). Animals were divided into three groups: sham‐operated group (Sham), ACLT + MMx group without LIPUS (−LIPUS), and ACLT + MMx group with LIPUS (+LIPUS; 30 mW/cm2, 20 min/day for 28 days). CTX‐II levels were elevated in both −LIPUS and +LIPUS groups compared to that in the Sham group after the operation, but there was no significant difference between +LIPUS and −LIPUS groups, suggesting that LIPUS does not affect the degradation of type II collagen in this model. In contrast, CPII was significantly increased in +LIPUS group compared to −LIPUS and Sham. Moreover, histological damage on the cartilage (Mankin score) was ameliorated by LIPUS, and type II collagen was immunohistochemically increased by LIPUS in the cartilage of an OA model. Of interest, mRNA expression of type II collagen was enhanced by LIPUS in chondrocytes. Together these observations suggest that LIPUS is likely to increase the type II collagen synthesis in articular cartilage, possibly via the activation of chondrocytes and induction of type II collagen mRNA expression, thereby exhibiting chondroprotective action in a rat OA model. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:361–369, 2010
Titanium uptake, induction of RANK‐L expression, and enhanced proliferation of human T‐lymphocytes
Authors: Cadosch Dieter , Sutanto Michael , Chan Erwin , Mhawi Amir , Gautschi Oliver P. , von Katterfeld Brilliana , Simmen Hans‐Peter , Filgueira Luis 
Published: October 2009

AbstractThere is increasing evidence that titanium ions are released from orthopedic implants by biocorrosion. The aim of this study was to investigate titanium uptake by human T‐lymphocytes and its effects on phenotype and proliferation. Freshly isolated human nonadherent peripheral blood mononuclear cells (NA‐PBMC), were exposed to TiCl4 [Ti(IV)]. Bioavailability and distribution of Ti(IV) in T‐lymphocytes was determined by energy‐filtered electron microscopy (EFTEM). The effects of Ti(IV) challenge on nonactivated and PHA‐activated cells were assessed by flow cytometric analysis of surface markers, RANK‐L production, and proliferation assays. EFTEM colocalized Ti(IV) with phosphorus in the nucleus, ribosomes, cytoplasmic membranes, and the surface membrane of T‐lymphocytes. Ti(IV) increased significantly the expression of CD69, CCR4, and RANK‐L in a concentration‐dependent manner. Titanium enters T‐lymphocytes through a currently unknown mechanism and binds to phosphorus‐rich cell structures. Titanium influences phenotype and function of T‐lymphocytes, resulting in activation of a CD69+ and CCR4+ T‐lymphocyte population and secretion of RANK‐L. These results strongly suggest the involvement of titanium ions challenged T‐lymphocytes in the complex pathophysiological mechanisms of aseptic loosening of orthopedic implants. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:341–347, 2010
Vibrational spectroscopy and crystal structure analysis of two polymorphs of the di‐amino acid peptide cyclo(L‐Glu‐L‐Glu)
Authors: Mendham Andrew P. , Palmer Rex A. , Potter Brian S. , Dines Trevor J. , Snowden Martin J. , Withnall Robert , Chowdhry Babur Z. 
Published: October 2009

AbstractCyclo(L‐Glu‐L‐Glu) has been crystallised in two different polymorphic forms. Both polymorphs are monoclinic, but form 1 is in space group P21 and form 2 is in space group C2. Raman scattering and FT‐IR spectroscopic studies have been conducted for the N,O‐protonated and deuterated derivatives. Raman spectra of orientated single crystals, solid‐state and aqueous solution samples have also been recorded. The different hydrogen‐bonding patterns for the two polymorphs have the greatest effect on vibrational modes with NH and CO stretching character. DFT (B3‐LYP/cc‐pVDZ) calculations of the isolated cyclo(L‐Glu‐L‐Glu) molecule predict that the minimum energy structure, assuming C2 symmetry, has a boat conformation for the diketopiperazine ring with the two L‐Glu side chains being folded above the ring. The calculated geometry is in good agreement with the X‐ray crystallographic structures for both polymorphs. Normal coordinate analysis has facilitated the band assignments for the experimental vibrational spectra. Copyright © 2009 John Wiley & Sons, Ltd.
Crystal structure and phonon modes of ilmenite‐type NaBiO3 investigated by Raman and infrared spectroscopies
Authors: Dias Anderson , Moreira Roberto L. 
Published: October 2009

AbstractNaBiO3 is an ilmenite‐type compound presenting a trigonal structure. In this work, we have performed optical spectroscopic investigations using Raman scattering and infrared reflectivity for this material. By using group‐theory calculations, it was possible to determine the number of optically active modes. Fitting procedures besides Kramers–Kronig (KK) procedures lead to a consistent collection of phonon modes. The Raman spectrum showed nine active first‐order modes, while the infrared one revealed eight polar phonons, in good agreement with the theoretical predictions. The results allowed us to confirm the R3 (#148) space group for this compound, and to establish a set of active phonons not yet reported in the literature. Copyright © 2009 John Wiley & Sons, Ltd.
Raman microscopy of haidingerite Ca(AsO3OH)·H2O and brassite Mg(AsO3OH)·4H2O
Authors: Frost Ray L. , Bahfenne Silmarilly , Čejka Jiří , Sejkora Jiří , Palmer Sara J. , Škoda Radek 
Published: October 2009

AbstractRaman spectroscopy has been used to study the arsenate minerals haidingerite Ca(AsO3OH)·H2O and brassite Mg(AsO3OH)·4H2O. Intense Raman bands in the haidingerite spectrum observed at 745 and 855 cm−1 are assigned to the (AsO3OH)2−ν3 antisymmetric stretching and ν1 symmetric stretching vibrational modes. For brassite, two similarly assigned intense bands are found at 809 and 862 cm−1. The observation of multiple Raman bands in the (AsO3OH)2− stretching and bending regions suggests that the arsenate tetrahedrons in the crystal structures of both minerals studied are strongly distorted. Broad Raman bands observed at 2842 cm−1 for haidingerite and 3035 cm−1 for brassite indicate strong hydrogen bonding of water molecules in the structure of these minerals. OH···O hydrogen‐bond lengths were calculated from the Raman spectra based on empirical relations. Copyright © 2009 John Wiley & Sons, Ltd.