bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and validation of RP‐HPLC‐fluorescence method for quantitative determination of quinidine, a probe substrate for P‐glycoprotein inhibition assay using Caco‐2 cell monolayer
Authors: Patil Anand G. , Reddy Dilip , D'Souza Russell , Damre Anagha 
Published: October 2009

AbstractA simple, sensitive and specific reverse‐phase high‐performance liquid chromatographic (RP‐HPLC) method with fluorescence detection was developed for quantitation of quinidine from HBSS buffer. The method was applicable in the bi‐directional transport assay for evaluation of the inhibitory effect of test compounds on P‐glycoprotein‐mediated quinidine transport; quinidine was used as a probe P‐glycoprotein substrate. The calibration curve was linear (correlation coefficient ≥99) in the range 0.30–100.00 nm. The method was validated and is specific and sensitive with limit of quantitation of 300 pm for quinidine. The method was found to be accurate and precise in the working calibration range. Stability studies were carried out at different storage conditions where the analyte was found to be stable. The applicability and reliability of the analytical method was evaluated by successful demonstration of efflux ratio (PappB → A/PappA → B) in the Caco‐2 cell monolayer efflux assay. The efflux ratio for quinidine (100 nm) alone was 10.8, which reduced to less than 2 in the presence of the classical P‐gp inhibitors verapamil and ketoconazole (100 μm each). Copyright © 2009 John Wiley & Sons, Ltd.
Sensitive LC‐MS‐MS method for the determination of tiopronin in human plasma
Authors: Jiang Bo , Xu Yu , Xu Fang , Sun Junjie , Li Na , Luo Yanan , Guo Qingxiang 
Published: October 2009

AbstractA highly selective and sensitive LC‐MS‐MS method was developed and validated to quantify tiopronin in human plasma, using fudosteine as the internal standard (IS). L‐Cysteine and 1,4‐dithiothreitol (DTT) were used as the reducer and the stabilizer to release and stabilify tiopronin from a dimmer and mix forms with endogenous thiols in the treatment of plasma samples. After a simple liquid–liquid extraction with ethyl acetate in acidic condition, the post‐treatment samples were analyzed on a C18 column interfaced with a triple‐quadruple tandem mass spectrometer using negative electrospray ionization. Methanol and water (40:60, v/v) were used as the isocratic mobile phase, with 0.2% formic acid and 1.0 mM tris (hydroxymethyl) aminomethane (Tris) in water. The method was validated to demonstrate the specificity, lower limit of quantification, accuracy and precision of measurements. The assay was linear over the concentration range 0.078–10 μg/mL. The correlation coefficients for the calibration curves ranged from 0.9980 to 0.9990. The intra‐ and inter‐day precisions, calculated from quality control samples, were not more than 10.49%. The method was employed in a pharmacokinetic study after oral administration of 200 mg tiopronin tablets to 24 healthy volunteers. Copyright © 2009 John Wiley & Sons, Ltd
The OstialPro positioning system for a renal artery stenosis
Authors: Igbokidi Oyidie , Jovin Ion S. 
Published: October 2009

AbstractAorto‐ostial lesions pose complex technical challenges in percutaneous revascularization procedures. The successful use of the ostial positioning system OstialPro has been reported in coronary arteries. This nitinol device has self expanding legs that open just distal to the guide catheter. This allows the stent to be deployed in the ostium of the vessel with the guide catheter positioned in the aorta and allows for accurate anatomic visualization of the plane between the ostium and the aorta. Besides coronary interventions, the OstialPro could be useful in peripheral vascular interventions for ostial renal, mesenteric or vertebral arteries stenoses. We report the case of a patient with a significant ostial renal artery stenosis who underwent a successful percutaneous intervention using the OstialPro device. © 2009 Wiley‐Liss, Inc.
Selected Examples of High‐Pressure Reactions in Glass Microreactors
Authors: Verboom W. 
Published: October 2009

AbstractRecent examples of high‐pressure organic reactions in (flow) glass microreactors of micrometer dimensions are described. Different types of reactions such as Diels‐Alder and nucleophilic aromatic substitution reactions performed in a capillary microreactor gave rate enhancements of 1.5–2.7 at 600 bar. Specifically designed flow glass microreactors, that withstand pressures of 140–690 bar, were used to study Diels‐Alder and oxidation reactions. The use of supercritical fluids gives rise to considerable rate enhancements, while the use of a transparent microreactor allows to visualize phase transitions.
An Adaptor Domain‐Mediated Autocatalytic Interfacial Kinase Reaction
Authors: Liao Xiaoli , Su Jing , Mrksich Milan 
Published: October 2009

AbstractThis paper describes a model system for studying the autocatalytic phosphorylation of an immobilized substrate by a kinase enzyme. This work uses self‐assembled monolayers (SAMs) of alkanethiolates on gold to present the peptide substrate on a planar surface. Treatment of the monolayer with Abl kinase results in phosphorylation of the substrate. The phosphorylated peptide then serves as a ligand for the SH2 adaptor domain of the kinase and thereby directs the kinase activity to nearby peptide substrates. This directed reaction is intramolecular and proceeds with a faster rate than does the initial, intermolecular reaction, making this an autocatalytic process. The kinetic non‐linearity gives rise to properties that have no counterpart in the corresponding homogeneous phase reaction: in one example, the rate for phosphorylation of a mixture of two peptides is faster than the sum of the rates for phosphorylation of each peptide when presented alone. This work highlights the use of an adaptor domain in modulating the activity of a kinase enzyme for an immobilized substrate and offers a new approach for studying biochemical reactions in spatially inhomogeneous settings.
Maltol‐Derived Ruthenium–Cymene Complexes with Tumor Inhibiting Properties: The Impact of Ligand–Metal Bond Stability on Anticancer Activity In Vitro
Authors: Kandioller Wolfgang , Hartinger Christian G. , Nazarov Alexey A. , Bartel Caroline , Skocic Matthias , Jakupec Michael A. , Arion Vladimir B. , Keppler Bernhard K. 
Published: October 2009

AbstractOrganometallic ruthenium–arene compounds bearing a maltol ligand have been shown to be nearly inactive in in vitro anticancer assays, presumably due to the formation of dimeric RuII species in aqueous solutions. In an attempt to stabilize such complexes, [Ru(η6‐p‐cymene)(XY)Cl] (XY=pyrones or thiopyrones) complexes with different substitution pattern of the (thio)pyrone ligands have been synthesized, their structures characterized spectroscopically, and their aquation behavior investigated as well as their tumor‐inhibiting potency. The aquation behavior of pyrone systems with electron‐donating substituents and of thiopyrone complexes was found to be significantly different from that of the maltol‐type complex reported previously. However, the formation of the dimer can be excluded as the primary reason for the inactivity of the complex because some of the stable compounds are not active in cancer cell lines either. In contrast, studies of their reactivity towards amino acids demonstrate different reactivities of the pyrone and thiopyrone complexes, and the higher stability of the latter probably renders them active against human tumor cells.
Modellbasierte Übertragung von satzweisem‐ auf kontinuierlichen Betrieb am Beispiel einer radikalischen Copolymerisation
Authors: Kröner T. , Lieske A. , Hahn M. , Bartke M. 
Published: October 2009

AbstractDie radikalische Copolymerisation von Diallyldimethylammoniumchlorid (DADMAC) und Acrylamid (AAM) ist durch sehr unterschiedliche Wachstumsgeschwindigkeiten der beiden Monomere gekennzeichnet. Daher erfolgte die Übertragung der Copolymerisation von Diallyldimethylammoniumchlorid und Acrylamid in Lösung von satzweisen auf kontinuierlichen Betrieb, mit der Zielsetzung, einheitliche Copolymere zu erhalten.
Depressive Symptoms and Risk Factors in Chinese Patients with Premature Ventricular Contractions without Structural Heart Disease
Authors: Liang Jin‐jun , Huang Cong‐xin , Yang Bo , Huang He , Wan Jun , Tang Yan‐hong , Zhao Qin‐yan 
Published: October 2009

AbstractHypothesisOur objective was to study depressive symptoms and potential risk factors in Chinese persons with premature ventricular contractions (PVCs) without structural heart disease.MethodsThe Zung self‐rating depression scale (ZSDS) was used to assess depressive symptoms. Correlations between depressive symptoms and sociodemographic and medical factors were analyzed by logistic regression.BackgroundPrevalence of depressive symptoms in coronary heart disease (CHD) is higher that in the general population and those for the majority of other chronic symptoms in patients with premature ventricular contractions (PUCS).ResultsOf 1,144 patients with PVCs (488 males, 656 females), age 51 ± 23 years, disease duration 1 mo − 23 y, a total of 309 (27%) patients were categorized as having depressive symptoms. Depressive symptoms increased with age, income, education level, nationality, PVC count/24h, society support, and settlement type (p < 0.05). Univariate logistic regression showed that being female, level of education, age, settlement type, and PVC count/24h significantly correlated with depressive symptoms (p < 0.05). Multivariate logistic regression indicated that 5 variables—female sex, education level, settlement type, age, and PVC count/24h significantly and independently related with depressive symptoms (p < 0.05).ConclusionsIn the Chinese population, depressive symptoms in subjects with PVCs were frequent. The village settlement type, female sex, age, PVC count/24h, and education level were independent risk factors for depressive symptoms. Further research on the relationship between PVCs and depressive symptoms in China is necessary. Copyright © 2009 Wiley Periodicals, Inc.
Ventilation‐Perfusion Mismatch Resulting From Iatrogenic Pulmonary Vein Stenosis After Radiofrequency Ablation: A Case Report
Authors: Wong Ka Kit , Gruenewald Simon M. , Larcos George 
Published: October 2009

AbstractA lobar ventilation‐perfusion mismatch is reported in a patient with right superior pulmonary vein stenosis, developing after radiofrequency ablation for atrial fibrillation. Radionuclide lung perfusion tomography was performed to assist with clinical management. Serial planar lung scintigraphy demonstrated no improvement in the perfusion abnormality despite interventional stenting. Iatrogenic pulmonary vein stenosis may potentially mimic pulmonary embolic disease. Copyright © 2009 Wiley Periodicals, Inc.
Prediction of Transition to Chronic Atrial Fibrillation in Elderly Patients with Nonvalvular Paroxysmal Atrial Fibrillation by Transthoracic Doppler Echocardiography
Authors: Sakabe Koichi , Fukuda Nobuo , Fukuda Yamato , Morishita Satofumi , Shinohara Hisanori , Tamura Yoshiyuki 
Published: October 2009

AbstractBackgroundIt is well known that paroxysmal atrial fibrillation (PAF) often precedes the development of chronic atrial fibrillation (CAF).HypothesisThe purpose of this study was to determine prospectively whether transthoracic echocardiography is useful for the prediction of the transition to CAF in elderly patients with nonvalvular PAF.MethodsForty‐two consecutive elderly patients (≥65 years) with nonvalvular PAF were prospectively evaluated after undergoing transthoracic echocardiography. The study endpoint was the transition to CAF (AF; ≥ 6 mo).ResultsDuring a follow‐up period of 32 ± 24 mo, 12 patients developed CAF. Patients with CAF had a significantly lower peak A velocity (A) and a higher E/A ratio of the transmitral inflow (TMF) such as a pseudonormalization pattern, and a lower peak atrial reversal wave velocity, higher peak diastolic wave velocity (D), and lower peak systolic/diastolic wave velocity ratio (S/D ratio) of the pulmonary venous flow (PVF). Kaplan‐Meier analysis revealed that the transition to CAF was observed more often when A was ≤ 70 cm/sec and E/A ratio was ≥ 1.07 of TMF, and D was ≥ 44 cm/sec and the S/D ratio was ≤ 1.34 of PVF. All patients developed CAF when the E/A ratio was ≥ 1.15 or the S/D ratio was ≤ 0.75.ConclusionsThis prospective study suggests that elderly patients at high risk for transition to CAF have a pseudonormalization pattern of TMF and a diastolic dominant pattern of PVF, and that transthoracic Doppler estimation of TMF and PVF may be useful in identifying elderly patients at high risk for the transition from nonvalvular PAF to CAF. Copyright © 2009 Wiley Periodicals, Inc.
Dual‐Function Multilayers for the Photodelivery of Nitric Oxide and Singlet Oxygen
Authors: Giancane Gabriele , Valli Ludovico , Sortino Salvatore 
Published: October 2009

AbstractIn this contribution we report the design, preparation and characterization of bichromophoric Langmuir–Schäfer multilayer films which enable the simultaneous generation of nitric oxide (NO) and singlet oxygen 1O2 (1Δg). They are achieved by exploiting effective interfacial electrostatic interactions between the floating film of a cationic amphiphilic nitric oxide (NO) photodonor and an anionic porphyrin dissolved in the water subphase. The morphology and the spectroscopic features of the floating films at the water–air interface are inspected by Brewster angle microscopy and UV/Vis reflection spectroscopy, respectively. Multilayer films containing both the chromogenic centres are obtained by horizontal lifting deposition onto hydrophobized quartz substrates and are characterized by steady‐state UV/Vis absorption, fluorescence and laser flash photolysis. The anionic porphyrin units are entangled into the films network mainly in their monomeric forms and no significant intra and inter‐chromophoric interaction occur in the excited state, preserving the photochemical independency of the two photoactive units in the multilayers. The capability of the bichromophoric films to delivery NO and singlet oxygen, 1O2 (1Δg), under light excitation is unambiguously demonstrated by the direct and in real time monitoring of these transient species using an ultrasensitive electrode and a time‐resolved infrared luminescence apparatus, respectively.
Disordered eating in adulthood is associated with reported weight loss attempts in childhood
Authors: Rubinstein Tamar B. , McGinn Aileen P. , Wildman Rachel P. , Wylie‐Rosett Judith 
Published: October 2009

AbstractObjective:To determine whether history of attempted weight loss in childhood (age ≤12 years) is associated with binge eating disorder (BED) and unhealthy weight loss behaviors in adults.Method:Cross‐sectional analysis from baseline visit data of 588 participants of a clinical trial. Data were collected from survey answers on health status, nutritional status, weight loss history, and weight loss behaviors.Results:History of childhood weight loss attempts was associated with high odds of BED in adults (odds ratio [OR] 3.4; 95% confidence interval [CI] 2.8–6.3) and high odds of unhealthy weight loss behaviors (OR 2.3; 95% CI 1.2, 2.6). A linear trend was observed in which young age at first attempted weight loss was associated with increased odds of both BED and unhealthy weight loss behaviors.Discussion:Weight loss attempts in childhood may be a risk factor for the development of unhealthy eating behaviors and BED in adults. © 2009 by Wiley Periodicals, Inc. (Int J Eat Disord 2010;)
A comparison of stigma toward eating disorders versus depression
Authors: Roehrig James P. , McLean Carmen P. 
Published: October 2009

AbstractObjective:The goal of this study was to compare the degree of stigma associated with anorexia nervosa (AN), bulimia nervosa (BN), and depression.Method:Participants read one of three vignettes describing clinical cases of AN, BN, or depression, and answered questions assessing stigma toward individuals with one of these three mental disorders.Results:Attitudes toward individuals with eating disorders were significantly more stigmatizing than attitudes toward individuals with depression. Individuals with an eating disorder were rated as more fragile, more responsible for their disorder, and more likely to use their disorder to gain attention than individuals with depression. Furthermore, the majority of participants reported that they admired certain aspects of eating disorders, thought that there might be some benefits to having an eating disorder, and that others would be motivated to imitate eating disorder behavior.Discussion:Stigma toward individuals with eating disorders is greater than stigma toward depression and includes unique features such as attitudes of envy. Implications of these results for the understanding of mental disorder stigma and eating disorders are discussed. © 2009 by Wiley Periodicals, Inc. (Int J Eat Disord 2010;)
Body dissatisfaction moderates weight curves in the inpatient treatment of Anorexia Nervosa
Authors: Vansteelandt Kristof , Pieters Guido , Vanderlinden Johan , Probst Michel 
Published: October 2009

AbstractObjectiveTo examine whether drive for thinness, body dissatisfaction, and restrictive/binge eating‐purging subtype at admission moderates the weight curves of patients with Anorexia Nervosa (AN) over the course of inpatient treatment.MethodThe nature of weight curves, individual differences herein and moderating factors are examined in 92 AN patients by means of multilevel modeling.ResultsThe average weight curve of AN patients is characterized by a linear weight increase during treatment that levels off near the end of treatment. Substantial individual differences exist in the shape of patients' weight curves. Patients with stronger body dissatisfaction at admission display a slower linear rate of weight gain over the course of treatment. Neither drive for thinness nor restrictive/binge eating‐purging subtype predicted patients' weight curve over the course of treatment.DiscussionBody dissatisfaction moderates patients' weight curves over the course of inpatient treatment but more research on factors determining weight curves is needed. © 2009 by Wiley Periodicals, Inc. Int J Eat Disord 2010; 43:694–700
Increased QT variability in patients with anorexia nervosa—An indicator for increased cardiac mortality?
Authors: Koschke Mandy , Boettger Michael K. , Macholdt Claudia , Schulz Steffen , Yeragani Vikram K. , Voss Andreas , Bär Karl‐Jürgen 
Published: October 2009

AbstractObjectiveIncreased mortality in anorexia nervosa is associated with autonomic dysfunction and prolongation of the QT interval. In this study, we examined the relative importance of repolarization abnormalities and vagal modulation of heart rate. In particular, we hypothesized that patients with anorexia nervosa show increased QT interval variability, particularly since this measure has been shown to correlate with serious cardiac arrhythmias.MethodWe assessed linear and nonlinear heart rate variability (HRV) parameters as well as measures of QT variability in 20 female patients with anorexia nervosa and 20 controls. In patients, parameters were correlated with serum electrolytes.ResultsQT variability was significantly increased in the patient group and correlated negatively with serum potassium concentrations. HRV measures showed a shift of autonomic balance towards vagal predominance.DiscussionThe increase in QT variability might at least in part account for the higher risk of cardiac arrhythmias in patients with anorexia nervosa. Once validated in a prospective study design, parameters of QT variability might serve as surrogate markers for arrhythmia risk stratification in anorexia nervosa. Supplementation with potassium might normalize QT variability abnormalities. © 2009 by Wiley Periodicals, Inc. Int J Eat Disord 2010; 43:743–750
Stabilization and Activation: New Alkyl Complexes of Zinc, Magnesium and Cationic Aluminium Featuring Chelating Bisguanidine Ligands
Authors: Reinmuth Matthias , Wild Ute , Rudolf Daniel , Kaifer Elisabeth , Enders Markus , Wadepohl Hubert , Himmel Hans‐Jörg 
Published: October 2009

AbstractIn this work we report on the synthesis of new zinc, magnesium and cationic aluminium alkyl as well as Mg aryl complexes featuring chelating bisguanidine ligands [2,8‐bis(tetramethylguanidino)naphthalene (btmgn) and 1,2‐bis(tetramethylguanidino)benzene (btmgb)]. The bond properties in the complexes are analyzed with a combination of experiments and quantum chemical calculations. VT NMR studies shed light on the fluxional processes within the complexes and their activation barriers. The compounds are stable against alkylation of the C=N imine bonds of the guanidino groups and might represent interesting alkyl transfer reagents and (co‐)catalysts. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Divalent and Multivalent Activation in Phosphate Triesters: A Versatile Method for the Synthesis of Advanced Polyol Synthons
Authors: Thomas Christopher D. , McParland James P. , Hanson Paul R. 
Published: October 2009

AbstractThe construction of mono‐ and bicyclic phosphate triesters possessing divalent and multivalent activation and their subsequent use in the production of advanced polyol synthons is presented. The method highlights efforts to employ phosphate tethers as removable, functionally active tethers capable of multipositional activation and their subsequent role as leaving groups in selective cleavage reactions. The development of phosphate tethers represents an integrated platform for a new and versatile tether for natural product synthesis and sheds light on new approaches to the facile construction of small molecules.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Synthesis and Substitution of 8‐(4,6‐Dichloropyrimidin‐5‐yl)‐BODIPY
Authors: Leen Volker , Schevenels Florian , Cui Jie , Xu Chan , Yang Wensheng , Tang Xiaoliang , Liu Weisheng , Qin Wenwu , De Borggraeve Wim M. , Boens Noël , Dehaen Wim 
Published: October 2009

Abstractmeso‐Dichloropyrimidyl‐BODIPY dyes are readily substituted by nucleophiles or through palladium‐catalysed coupling reactions, while retaining excellent quantum yields of fluorescence. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Wittig‐Type Olefination of Alcohols Promoted by Nickel Nanoparticles: Synthesis of Polymethoxylated and Polyhydroxylated Stilbenes
Authors: Alonso Francisco , Riente Paola , Yus Miguel 
Published: October 2009

AbstractNickel nanoparticles were found to promote the Wittig‐type olefination of primary alcohols with phosphorus ylides. The latter can be prepared from the corresponding phosphonium salts with nBuLi or in situ generated with lithium metal. The methodology is especially efficient for the synthesis of stilbenes and is applied in the absence of any additive as ahydrogen acceptor. A new approach to the synthesis of polymethoxylated and polyhydroxylated stilbenes, including resveratrol, DMU‐212 and analogues, is presented.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Lactate Biosensor Based on Hydrotalcite‐Like Compounds: Performances and Application to Serum Samples
Authors: Carpani Irene , Guadagnini Lorella , Tonelli Domenica 
Published: October 2009

AbstractA lactate biosensor based on lactate oxidase supported onto a hydrotalcite, electrochemically deposited on a platinum surface, was developed for the first time. For the best electrode configuration, a linear response up to 0.8 mM, with a limit of detection of 14 μM and a sensitivity of 91 mA M−1 cm−2, was obtained. The influence of some interferents due to the oxidation of hydrogen peroxide (at +0.35 V vs. SCE) was also studied. By controlling carefully the experimental conditions, the determination of lactate in a commercial serum sample in the presence of interferents was successfully accomplished.