bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunoregulation by tumor necrosis factor superfamily member LIGHT
Authors: Wang Yugang , Zhu Mingzhao , Miller Mendy , Fu Yang‐Xin 
Published: April 2009

Summary:  LIGHT (homologous to lymphotoxins, inducible expression, competes with herpesvirus glycoprotein D for herpesvirus entry mediator, a receptor expressed on T lymphocytes) is a member of the tumor necrosis factor superfamily that contributes to the regulation of immune responses. LIGHT can influence T‐cell activation both directly and indirectly by engagement of various receptors that are expressed on T cells and on other types of cells. LIGHT, LIGHT receptors, and their related binding partners constitute a complicated molecular network in the regulation of various processes. The molecular cross‐talk among LIGHT and its related molecules presents challenges and opportunities for us to study and to understand the full extent of the LIGHT function. Previous research from genetic and functional studies has demonstrated that dysregulation of LIGHT expression can result in the disturbance of T‐cell homeostasis and activation, changing the ability of self‐tolerance and of the control of infection. Meanwhile, blockade of LIGHT activity can ameliorate the severity of various T‐cell‐mediated diseases. These observations indicate the importance of LIGHT and its involvement in many physiological and pathological conditions. Understanding LIGHT interactions offers promising new therapeutic strategies that target LIGHT‐engaged pathways to fight against cancer and various infectious diseases.
Evaluation of chlorhexidine substantivity on salivary flora by epifluorescence microscopy
Authors: Tomás I , García‐Caballero L , Cousido MC , Limeres J , Álvarez M , Diz P 
Published: April 2009

Objective:  To evaluate the in vivo antimicrobial activity of chlorhexidine (CHX) in saliva 7 h after its application using an epifluorescence microscopy technique.Subjects and methods:  Fifteen volunteers performed a single mouthrinse with sterile water (SM‐water) and with 0.2% CHX (SM‐0.2% CHX). Saliva samples were taken at 30 s and 1, 3, 5 and 7 h after each application. The bacterial suspension was mixed with the SYTO 9/propidium iodide staining and observed using an Olympus BX51 microscope. The mean percentage of viable bacteria was calculated for each sample.Results:  In comparison with baseline values, the frequency of viable bacteria decreased significantly at 30 s after the SM‐0.2% CHX (P < 0.001) and presented significant antibacterial activity up to 7 h after the mouthrinse (P < 0.001). In comparison with SM‐water, the prevalence of viable bacteria was significantly lower at 30 s after the SM‐0.2% CHX (P < 0.001) and showed a significant antibacterial effect up to 7 h after the mouthrinse (P < 0.001).Conclusions:  Epifluorescence microscopy permits evaluating the antimicrobial activity of CHX on the salivary flora in real‐time. Fluorescence assays could be particularly useful to analyse simultaneously the effect of antimicrobials that alter the cytoplasmic membrane integrity on different oral ecosystems.
Occupational airborne contact allergy to tetrazepam in a geriatric nurse
Authors: Breuer Kristine , Worm Margitta , Skudlik Christoph , Schröder Claudia , John Swen Malte 
Published: April 2009

SummaryA 52‐year‐old geriatric nurse presented with recurrent eczema localized in uncovered skin areas. Patch testing produced an eczematous skin reaction with type IV sensitization to tetrazepam. A relapse of contact dermatitis was successfully prevented by using occupational skin protection measures and organizational measures. Our case indicates that a sensitization to drugs should be considered when allergic contact dermatitis is suspected in nursing personnel.
Predicting health‐related quality of life of parents of children with inherited metabolic diseases
Authors: Hatzmann Janneke , Valstar Marlies J , Bosch Annet M , Wijburg Frits A , Heymans Hugo SA , Grootenhuis Martha A 
Published: April 2009

AbstractAim:  The aim of this study was to examine medical, socio‐demographic and psychosocial determinants of health‐related quality of life (HRQoL) of parents of children with metabolic diseases.Methods:  A survey among parents of children with metabolic diseases (children aged 1–19 years, diagnosed >1 year before the start of the study, living at home). Parents were approached through the Emma Children's Hospital, and through a national parent and patient association. HRQoL was assessed using the TNO‐AZL Questionnaire for Adult's Health Related Quality of Life (TAAQOL), describing 12 domains of HRQoL. Predictor variables were taken from a self‐report questionnaire. Univariate and multivariate logistic regression analyses were performed to predict which parents were at risk for HRQoL impairment.Results:  Mainly psychosocial determinants were predictive for parental HRQoL. Emotional support was protective for parental HRQOL while loss of friendship was a risk factor for HRQoL impairment. Medical and socio‐demographic variables did not consistently predict parental HRQoL.Conclusion:  Psychosocial determinants appeared more important in predicting parental HRQoL than medical and socio‐demographic variables. Interventions should be focused on supporting parents combining the care for their children with a social life. Further research on this subject is necessary. In the meantime, involved medical specialists should pay structural attention to parental functioning.
Genetic polymorphisms in Thai neonates with hyperbilirubinemia
Authors: Prachukthum Sariya , Nunnarumit Pracha , Pienvichit Paneeya , Chuansumrit Ampaiwan , Songdej Daunthida , Kajanachumpol Saowanee , Pakakasama Samart , Hongeng Suradej 
Published: April 2009

AbstractAim:  Polymorphisms of the UGT1A1 gene, SLCO1B1 gene and GST gene have been associated with significant hyperbilirubinemia. We would like to determine whether the variation of UGT1A1 gene, SLCO1B1 gene and GST gene may play a significant role in neonatal hyperbilirubinemia in Thai infants.Methods:  Ninety‐one study subjects (hyperbilirubinemic group) and 86 control subjects were studied.Results:  The cause of neonatal hyperbilirubinemia could not be identified in 64 infants (70.3%), ABO blood group incompatibility in 14.3% and Glucose‐6‐phosphate dehydrogenase (G‐6‐PD) deficiency in 8.8%. In the hyperbilirubinemic group, 23 of 91 (25.3%) infants demonstrated variant of UGT1A1 at nucleotides (nt) 211 as compared to 6 of 86 (7%) in the control group (p = 0.001). There were no significant differences between groups in the variants UGT1A1 at nt 686, SLCO1B1 gene at nt 388, 463 and the GST gene. Male infants with G‐6‐PD deficiency were associated with hyperbilirubinemia (21.2% vs. 4.8% in the control group) with an odds ratio (OR) of 5.37 (p =0.02). The relationship between G‐6‐PD and variant in UGT1A1 gene at nt 211 could not be determined.Conclusion:  Thai infants with variant in the UGT1A1 at nt 211 or with G‐6‐PD deficiency are at higher risk for developing neonatal hyperbilirubinemia.
α‐Adrenoceptor agonists for the treatment of vasovagal syncope: a meta‐analysis of worldwide published data
Authors: Liao Ying , Li Xueying , Zhang Yanwu , Chen Stella , Tang Chaoshu , Du Junbao 
Published: April 2009

AbstractAim:  The present study was aimed at evaluating present randomized controlled trials (RCTs) regarding the effect of α‐adrenoceptor agonists on vasovagal syncope (VVS).Methods:  According to inclusion and exclusion criteria, articles were selected from medical electronic databases. RCTs were then assessed based on the Juni assessment, and meta‐analysis was completed using the Review Manager 4.2 software. Indication to further evaluate effects was the recurrence of syncope during follow‐up treatment or a response in the head‐up tilt test (HUT) after treatment. The results were stated as odd ratio (OR), with a 95% confidence interval (CI) and a  p < 0.05 significant level.Results:  In total, six RCTs were selected. Funnel plot analysis showed possible publication bias. Meta‐analysis of the six RCTs, including all 165 patients in the treatment group and 164 patients in the control group, indicated that α‐adrenoceptor agonists were more effective than placebos in treating VVS (OR = 0.21, 95% CI: 0.06–0.77, p = 0.02). The further, weighted independent t‐test disclosed that the weighted mean percentage of responders for midodrine (76.3%± 7.7%) was significantly higher than that for etilefrine (65.5%± 15.4%) (t = 5.863, p < 0.001).Conclusion:  The currently published RCTs support that α‐adrenoceptor agonists might be effective for VVS. Midodrine can be regarded as a better choice compared with etilefrine.
Natural evolution of regurgitation in healthy infants
Authors: Hegar Badriul , Dewanti Nita R , Kadim Muzal , Alatas Safira , Firmansyah Agus , Vandenplas Yvan 
Published: April 2009

AbstractObjectives:  To determine the natural history of infant regurgitation during the first year of life.Methods: Parents recorded prospectively the frequency of regurgitation for 1 week before consultation during the first year of life. A sub‐group analysis according to the method of feeding was planned.Results:  A total of 130 infants of an original sample of 163 (80%) newborns were followed up for 1 year. Daily spilling was highest during the first month of life (73%) and decreased gradually to 50% during the fifth month of life. During the first 2 months of life, 20% of the infants regurgitated more than four times per day. After the age of 12 months, only 4% of the infants had daily regurgitations. Exclusively breastfed infants did regurgitate less than partially breastfed infants. Weight gain was influenced by the frequency of regurgitation, especially in partially breastfed infants.Conclusion: Regurgitation in infancy is common, decreasing from birth, and tends to disappear by 12 months of age. Weight gain during the first 4 months of life is decreased in infants who regurgitate more than four times a day. Our data suggest that exclusively breastfed infants regurgitate less than partially breastfed babies.
Mineral solubility of Panicum maximum with four herbaceous forage legume mixtures incubated in the rumen of N' Dama steers
Authors: AJAYI Festus T. , BABAYEMI Olaniyi Jacob , TAIWO Abiodun A. 
Published: April 2009

ABSTRACTThis study was designed to determine the mineral concentration and bioavailability of iron (Fe), phosphorus (P), calcium (Ca) and sodium (Na) of mixtures of Panicum maximum (Panicum) with Lablab purpureus (Lablab), Centrosema pubescens (Centro), Stylosanthes guianensis (Stylo), and Aeschynomene histrix (Histrix) in a fistulated N'Dama steer. The solubility of minerals was determined by an in sacco method by incubating ground samples of these forages in the rumen at 0, 6, 12, 24, 48 and 72 h. The mineral solubility characteristics of the forages were evaluated using the equation PD = a + b (1 − e−ct). The result showed that Panicum intercropped with legumes had better crude protein (CP) (av. 9.22% DM) and ash content (av. 2.22% DM) than sole Panicum which gave 7.63% DM and 1.84% DM for CP and ash contents respectively. The highest value of CP of 23.29% DM and ash content (3.96% DM) was obtained for Lablab. The mineral content values of the intercrop were higher than the sole Panicum. Higher mineral content values were obtained in the sole legumes. Iron solubility values of b and potential degradation (PD) which were 58.71% and 87.92% respectively were for Panicum/Histrix mixtures. Calcium solubility values ranged from 30.0% in Panicum to 46.0% in Panicum/Lablab mixture for b fraction. P solubility ranged from 28.0% and 46.0% for b and PD fractions in Panicum to 43.0% and 71.0% in Panicum/Centro mixture respectively. The solubility of the legumes was higher in Na for b fraction (av. 42.0%) and PD fraction (av. 60.0%). Higher values of b (av. 38.0%) and PD (av. 52.0%) were obtained for the mixtures than for sole Panicum. This study revealed that intercropping Panicum with either of the herbaceous forage legumes used for this experiment would increase the Ca, P, Na and Fe contents of the grass.
Death losses due to stillbirth, neonatal death and diseases in cloned cattle derived from somatic cell nuclear transfer and their progeny: a result of nationwide survey in Japan
Authors: WATANABE Shinya , NAGAI Takashi 
Published: April 2009

ABSTRACTTo obtain the data concerning death losses due to stillbirth, neonatal death and diseases in cloned cattle derived from somatic cell nuclear transfer (SCNT) and their progeny produced by Japanese institutions, a nationwide survey was carried out in July‐August, 2006. As a result, lifetime data concerning 482 SCNT cattle (97.5% of cattle produced in the country at that time) and 202 progeny of SCNT cattle were accumulated and the death loss of these cattle was analyzed. Although 1/3 of delivered SCNT calves died during the perinatal period due to stillbirth and neonatal death, incidence of death loss due to diseases in SCNT cattle surviving more than 200 days after birth seems to be the same as these in conventionally bred cattle. In contrast, progeny of SCNT cattle showed the same level in death loss as observed in conventionally bred cattle throughout their lifetime. These results suggest that robust health would be expected in SCNT cattle surviving to adulthood and their progeny.
Using a complex non‐TDN based model (the DVE/OEB system) to predict microbial protein synthesis, endogenous protein, degradation balance, and total truly absorbed protein supply of different varieties of cereal oats for ruminants
Authors: YU Peiqiang , NIU Zhiyuan 
Published: April 2009

ABSTRACTRecently a new super‐genotype of oat has been developed in the Crop Development Center called CDC SO‐I (‘SuperOat’: low lignin and high fat). In a previous study, we evaluated total metabolizable protein using a TDN‐based model‐NRC‐2001 which is popular in North America. However, the TDN‐based NRC model is not accepted universally. The objectives of this study were to use a complex non‐TDN based model, the DVE/OEB system, to evaluate potential nutrient supply to ruminants from the SuperOat in comparison with two normal varieties of oats (CDC Dancer and Derby) in western Canada. The quantitative predictions were made in terms of: (i) truly absorbed rumen synthesized microbial proteins in the small intestine; (ii) truly absorbed rumen undegraded feed protein in the small intestine; (iii) endogenous protein in the digestive tract; (iv) total truly absorbed protein in the small intestine; and (v) protein degraded balance. Results showed that using the DVE/OEB system to predict the potential nutrient supply, it was found that the SuperOat had similar truly absorbed rumen synthesized microbial protein levels (61, 63, 59 g/kg DM, P > 0.05, for SuperOat, CDC Dancer and Derby, respectively), higher truly absorbed rumen undegraded feed protein than CDC Dancer (22 vs. 17 g/kg DM P < 0.05, for SuperOat, CDC Dancer, respectively), but similar to Derby (22 vs. 21 g/kg DM; P > 0.05), and similar endogenous protein (16, 16, 18 g/kg DM; P > 0.05). Total truly absorbed protein in the small intestine is only numerically higher in the SuperOat (67 vs. 65, 62 g/kg DM, P > 0.05, for CDC Dancer and Derby, respectively). However, the protein degraded balance was significantly different (P < 0.05) with a positive value for the SuperOat (7.0 g/kg DM) and negative values for two normal varieties (−1.5, −6.8 g/kg DM for CDC Dancer and Derby, respectively). In conclusion, the model predicted significantly different protein degradation balance. The SuperOat had positive degradation balance but other two normal varieties had negative protein degraded balance However, the SuperOat had similar total absorbed protein value to the two normal varieties of oats.
Influence of orally administered bovine lactoferrin on lipid metabolism in lipopolysaccharide‐injected preruminant calves
Authors: KUSHIBIKI Shiro , SHINGU Hiroyuki , KOMATSU Tokushi , ITOH Fumiaki , MORIYA Naoko , TOUNO Eiko , OSHIBE Akinori , HODATE Koichi 
Published: April 2009

ABSTRACTThe aim of this study was to investigate the influence of oral lactoferrin (LF) administration on lipid metabolism changes in calves given lipopolysaccharide (LPS). Twenty‐one 4‐day‐old Holstein calves were divided into three groups, with each group receiving one of three oral doses of LF (0, 1, 3 g/day) for 10 consecutive days (day −10 to day −1). All calves were intravenously injected with LPS (50 ng/kg BW) on day 0, the day after LF treatment ended. Plasma triglyceride concentrations were lower (P < 0.05) in the LF‐treated calves than in the control calves given 0 g/day of LF at 12 and 24 h after LPS injection. Plasma NEFA concentrations were elevated between 6 and 24 h after LPS treatment. At 12 h, the concentration of plasma NEFA was lower (P < 0.05) in the calves given LF 3 g/day than in the control calves. On day 0, plasma total cholesterol and phospholipid concentrations tended to be lower in the LF groups administered 1 and 3 g of LF/day than in the control group, but did not differ significantly among the groups. The plasma very‐low‐density and low‐density lipoprotein concentrations were lower (P < 0.05) at 12, 24, and 72 h in the LF groups than in the control calves. The concentrations of plasma high‐density lipoprotein tended to be lower in the LF groups than in the control group between day 0 and 96 h, though there were no significant group differences. The concentration of plasma interleukin‐1β was lower (P < 0.05) in the calves fed LF 3 g/day than in the control calves at 2 and 12–48 h after LPS injection. These data suggest that LF inhibits LPS‐induced alterations in lipid metabolism in preruminant calves.
Application of cycling index and input‐output environs for interpretation of nutrient flows in mixed rice‐beef production systems in Japan
Authors: TABATA Yusuke , OISHI Kazato , KUMAGAI Hajime , HIROOKA Hiroyuki 
Published: April 2009

ABSTRACTThe objective of the present study was to apply two methods developed in ecology, the cycling index and input‐output environs, to interpret nutrient flows in mixed rice‐beef production systems. The cycling index (CI) was a quantitative measure of nutrient cycling. It was defined as the proportion of cycled nutrients to the total amount of nutrient flows. On the other hand, the input‐output environs provide a quantitative distribution on a particular input or output. In this study, these methods were applied to the nutrient flows in the mixed rice‐beef production systems in Japan. The results of CI provided information on the effects of nutrient cycling on the efficient conversion of nutrient imports to nutrient export. The results of input‐output environs indicated that the indices represent indirect effects provided by the interaction between rice and beef production. In conclusion, these methods indicated new findings on nutrient utilization in the systems. The results of this study implied the further applicability of these two methods to the study of nutrient flows in mixed crop‐animal production systems.
Squamous cell carcinoma arising from perineal lesion in a familial case of Hidradenitis suppurativa
Authors: Chandramohan K , Mathews Anitha , Kurian Anu , Thomas Shaji , Ramachandran K 
Published: April 2009

ABSTRACTHidradenitis suppurativa (HS) is a chronic dermatologic disorder characterised by multiple vesiculopustular cutaneous lesions in skin. It sometimes express autosomal dominant mode of inheritance. In some patients, it can give rise to squamous cell carcinoma of skin. Unlike the usual marjolin's ulcer which develops in chronic scars, this disease carries a grave prognosis with high recurrence rate and mortality.Here we are reporting the case of a 40‐year‐old gentle man with hidradenitis suppurativa, who presented with squamous cell carcinoma in the perianal region, and had multiple family members affected with HS. A short history and review of literature are discussed. Squamous cell carcinoma arising from HS is a major therapeutic challenge. This malignancy carries very high rates of recurrence and mortality. Careful surveillance of the lesions of HS, especially occurring in perineal region is very important to diagnose secondary malignancies. Similarly, meticulous surgical excision with careful resection of all sinus tracks and frozen section examination of margins are very important to eradicate the disease.
The use of plasma rich in growth factors on wound healing in the skin: experimental study in rabbits
Authors: Molina‐Miñano Francesco , López‐Jornet Péa , Camacho‐Alonso Fabio , Vicente‐Ortega Vicente 
Published: April 2009

ABSTRACTThe objective of this study was to use autologous plasma rich in growth factors (PRGF) on wound healing in the skin in New Zealand albino rabbits and to study reepithelialisation and inflammation at 7 and 28 days. A prospective study carried out on 20 adult rabbits. Two wounds were made on the in the skin on the back of each animal; one control, and the other in which PRGF was applied. The PRGF preparation was obtained from 10 ml of whole blood. The reepithelialisation and inflammation of wounds were measured at 7 and 28 days. Reepithelialisation improved in skin at 7 days (P = 0·007), with resolution of the inflammatory process (P = 0·005), having significant differences with respect to the control. Therefore, PRGF accelerates reepithelialisation and reduces inflammation at 7 days in skin.
The efficacy and safety of epidermal growth factor in treatment of diabetic foot ulcers: the preliminary results
Authors: Tuyet Hoa Le , Nguyen Quynh Tram Tran , Vo Hoang Minh Hien , Thi Bich Dao Nguyen , Do Dinh Thang , Le Tan Duc , Van Hoang Lam , Le Huy Truong , Doan Huu Hau , Tran Trong Tri Nguyen 
Published: April 2009

ABSTRACTObjective: To evaluate the efficacy and safety of recombinant human epidermal growth factor (rh‐EGF) in healing foot ulcers in diabetic patients.Methods: A total of 28 subjects with foot ulcers were recruited into the pilot study. Patients who had obvious peripheral arterial disease, trans‐tibial amputation, plastic surgery or skin flap, and skin graft were excluded. The properly debrided wounds and the non closure wounds after toe amputation were included. When the wounds became clean or uninfected, they received twice‐a‐day treatment with 0·005% Easyef and hydrocolloid dressing. The size and severity of the wounds were evaluated. Others such as blood sugar, renal and hepatic function, serum albumin, vascular condition, foot infection or osteomyelitis were assessed.Results: All of 28 patients had positive response of granulation (100%). Complete healing was noted in 13 out of 23 subjects and finished 8‐week follow‐up (56·5%). The rates of wound closure were 43·3%, 59·9%, 68·7%, and 84·8% in week 2, 4, 6 and 8, respectively, regardless of the severity. Being dropped out, three patients needed further interventions. No skin allergic reaction. Over‐granulation was observed in one female patient (3·7%), but as minor.Conclusions: Easyef has positive effects on healing of moderate‐to‐severe foot ulcers and demonstrated being safe to diabetic patients. The drug had high tolerability and compliance.