bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring patient perspectives on electronic patient-reported outcome measures in home-based cancer palliative care: A qualitative study
Authors: Consolo Letteria , Basile Ilaria , Colombo Stella , Rusconi Daniele , Pasquot Loredana , Campa Tiziana , Caraceni Augusto , Lusignani Maura 
Published: April 2024

Background Electronic patient-reported outcomes (ePROMs) enhance symptom management and patients’ engagement in palliative cancer care. However, integrating them into this setting brings challenges, including patients’ familiarity with technological devices and declining health status. Prioritizing the patient's acceptability and feasibility is crucial for their adoption. However, more knowledge is needed about patients’ perspectives on the adoption of ePROMs in the community, especially for home-based palliative care. Aim Explore patient viewpoints on utilizing ePROMs for symptom reporting in home-based oncology palliative care. Design A qualitative interpretative approach was used to evaluate patients’ points of view on using ePROMs in this specific care setting. Semistructured interviews were carried out. Data were analyzed using a reflexive thematic analysis. Setting/participants A total of 25 patients receiving oncological home palliative care from the advanced palliative care unit of the Fondazione IRCCS Istituto Nazionale dei Tumori in Milan, Italy, were invited to participate. Twenty interviews were conducted, as five patients declined due to deteriorating health. Results Four themes were identified: (1) strategic value of ePROMs and subjective appreciation; (2) enhancing patient centeredness through ePROMs; (3) exploring and addressing concerns about the use of ePROMs and (4) intersecting factors influencing the efficacy of ePROMs Conclusion Despite initial reticence, home palliative care patients consider ePROMs as potentially valuable allies monitoring symptoms, enhancing their quality of life, and amplifying their voices on less explored aspects of care. Continuous dialog between healthcare professionals and patients is crucial for addressing patient skepticism about ePROMs and their impact on the human aspect of care.
Effect of wrist orthoses on upper limb function, activities of daily living, and stress response
Authors: Hirata Junya , Yoshimura Manabu , Inoue Keiko 
Published: April 2024

Introduction This study examined the effects on upper limb function, activities of daily living, and stress responses when wearing a wrist orthosis made of padded fiberglass or thermoplastic and provided essential information for selecting an orthosis. Methods Thirty-one healthy adults performed two tests while not wearing a wrist orthosis, wearing a padded fiberglass wrist orthosis, and wearing a thermoplastic wrist orthosis. The Purdue Pegboard Test examined upper limb control. In the second test, the actions indicated by the Hand20 questionnaire were performed while wearing a wrist orthosis. An electrocardiogram was obtained before and after each test to identify any changes in sympathetic nervous system activity. Results The Purdue Pegboard Test scores were significantly higher when not wearing a wrist orthosis than when wearing wrist orthosis, and the Hand20 scores for all question were significantly lower. Thermoplastic wrist orthoses had fewer restrictions for upper limb function compared to padded fiberglass wrist orthoses, however activities of daily living were more limited. The low frequency/high frequency ratio and high frequency measures showed no significant differences. Conclusions Pegboard test scores and the Hand 20 scores suggest that a wrist orthosis causes restriction of upper limb function.
Navigating the outcome maze: a scoping review of outcomes and instruments in clinical trials in genetic neurodevelopmental disorders and intellectual disability
Authors: Müller Annelieke R. , van Silfhout Nadia Y. , den Hollander Bibiche , Kampman Dick H. C. , Bakkum Lianne , Brands Marion M. M. G. , Haverman Lotte , Terwee Caroline B. , Schuengel Carlo , Daams Joost , Hessl David , Wijburg Frits A. , Boot Erik , van Eeghen Agnies M. 
Published: April 2024

Background: Individuals with genetic neurodevelopmental disorders (GNDs) or intellectual disability (ID) are often affected by complex neuropsychiatric comorbidities. Targeted treatments are increasingly available, but due to the heterogeneity of these patient populations, choosing a key outcome and corresponding outcome measurement instrument remains challenging. Objectives: The aim of this scoping review was to describe the research on outcomes and instruments used in clinical trials in GNDs and ID. Eligibility criteria: Clinical trials in individuals with GNDs and ID for any intervention over the past 10 years were included in the review. Sources of evidence: MEDLINE, PsycINFO, and Cochrane CENTRAL were searched. Titles and abstracts were independently screened for eligibility with a subsample of 10% double-screening for interrater reliability. Data from full texts were independently reviewed. Discrepancies were discussed until consensus was reached. Charting methods: Information was recorded on patient populations, interventions, designs, outcomes, measurement instruments, and type of reporter when applicable. Qualitative and descriptive analyses were performed. Results: We included 312 studies reporting 91 different outcomes, with cognitive function most frequently measured (28%). Various outcome measurement instruments ( n = 457) were used, with 288 in only a single clinical trial. There were 18 genetic condition-specific instruments and 16 measures were designed ad-hoc for one particular trial. Types of report included proxy-report (39%), self-report (22%), clinician-report (16%), observer-report (6%), self-assisted report (1%), or unknown (16%). Conclusion: This scoping review of current practice reveals a myriad of outcomes and outcome measurement instruments for clinical trials in GNDs and ID. This complicates generalization, evidence synthesis, and evaluation. It underlines the need for consensus on suitability, validity, and relevancy of instruments, ultimately resulting in a core outcome set. A series of steps is proposed to move from the myriad of measures to a more unified approach.
The migration and occupation of Li+ and Na+ in illite and montmorillonite during the heating process
Authors: Wu Zhenxiao , Li Shangying , Wang Yang , Cheng Hongfei 
Published: April 2024

AbstractThe impact of temperature on the migration of cations within layers of clay minerals is of profound significance for the design and practical application of materials derived from clay minerals. This study focuses on Li+ and Na+ as representative cations, together with illite (Ilt) and montmorillonite (Mnt) as representative clay minerals. The study investigates the behaviour of cation migration and occupation within clay minerals across varying temperatures. A series of samples were prepared meticulously by immersing illite and montmorillonite in Li+ and Na+ solutions, subsequently subjecting them to different temperatures (unheated, 100, 150, 200, 250 and 300°C) for 24 h. Through the use of techniques such as X-ray diffraction (XRD), cation exchange capacity (CEC), Fourier-transform infrared spectroscopy (FTIR), magic angle rotating solid nuclear magnetic resonance spectroscopy (MAS NMR), and X-ray photoelectron spectroscopy (XPS), the study discerns structural transformations in illite and montmorillonite, and tracks the migration and occupation of Li+ and Na+. The findings reveal that following heating, Na+ and Li+ do not infiltrate the lattice of illite. For montmorillonite, Na+ also does not migrate into the montmorillonite lattice, however, in contrast, Li+ does exhibit migration into this lattice. Notably, the migration and occupation of interlayer Li+ within montmorillonite exhibit discernible temperature dependence. Specifically, upon reaching 150°C, interlayer Li+ migrate to ditrigonal cavities within the tetrahedral layers. As the temperature elevates to 200°C, Li+ further permeate vacant octahedral sites through the ditrigonal cavities, culminating in the formation of a localised trioctahedral structure.
Risk stratification refinements with inclusion of haemodynamic variables at follow-up in patients with pulmonary arterial hypertension
Authors: Boucly Athénaïs , Beurnier Antoine , Turquier Ségolène , Jevnikar Mitja , de Groote Pascal , Chaouat Ari , Cheron Céline , Jaïs Xavier , Picard François , Prévot Grégoire , Roche Anne , Solinas Sabina , Cottin Vincent , Bauer Fabrice , Montani David , Humbert Marc , Savale Laurent , Sitbon Olivier 
Published: April 2024

BackgroundHaemodynamic variables are prognostic factors in pulmonary arterial hypertension (PAH). However, right heart catheterisation (RHC) is not systematically recommended to assess the risk status during follow-up. This study aimed to assess the added value of haemodynamic variables in prevalent patients to predict the risk of death or lung transplantation according to their risk status assessed by the non-invasive four-strata model as recommended by the European guidelines.MethodsWe evaluated incident patients with PAH enrolled in the French pulmonary hypertension registry between 2009 and 2020 who had a first follow-up RHC. Cox regression identified, in each follow-up risk status, haemodynamic variables significantly associated with transplant-free survival. Optimal thresholds were determined by time-dependent receiver operating characteristics. Several multivariable Cox regression models were performed to identify the haemodynamic variables improving the non-invasive risk stratification model.ResultsWe analysed 1240 incident patients reassessed within 1 year by RHC. None of the haemodynamic variables were significantly associated with transplant-free survival among low-risk (n=386) or high-risk (n=71) patients. Among patients at intermediate (intermediate-low, n=483 and intermediate-high, n=300) risk at first follow-up, multivariable models including either stroke volume index (SVI) or mixed venous oxygen saturation (SvO2) were the best. The prognostic performance of a refined six-strata risk stratification model including the non-invasive four-strata model and SVI >37 mL·m−2and/orSvO2>65% for patients at intermediate risk (area under the curve (AUC) 0.81; c-index 0.74) was better than that of the four-strata model (AUC 0.79, p=0.009; c-index 0.72).ConclusionCardiopulmonary haemodynamics may improve risk stratification at follow-up in patients at intermediate risk.
CHMP5 attenuates osteoarthritis via inhibiting chondrocyte apoptosis and extracellular matrix degradation: involvement of NF-κB pathway
Authors: Gao Weilu , Liu Rui , Huang Keke , Fu Wenhan , Wang Anquan , Du Gongwen , Tang Hao , Yin Li , Yin Zongsheng S. 
Published: April 2024

Abstract
Background
Osteoarthritis (OA), the most common joint disease, is linked with chondrocyte apoptosis and extracellular matrix (ECM) degradation. Charged multivesicular body protein 5 (CHMP5), a member of the multivesicular body, has been reported to serve as an anti-apoptotic protein to participate in leukemia development. However, the effects of CHMP5 on apoptosis and ECM degradation in OA remain unclear.

Methods
In this study, quantitative proteomics was performed to analyze differential proteins between normal and OA patient articular cartilages. The OA mouse model was constructed by the destabilization of the medial meniscus (DMM). In vitro, interleukin-1 beta (IL-1β) was used to induce OA in human chondrocytes. CHMP5 overexpression and silencing vectors were created using an adenovirus system. The effects of CHMP5 on IL-1β-induced chondrocyte apoptosis were investigated by CCK-8, flow cytometry, and western blot. The effects on ECM degradation were examined by western blot and immunofluorescence. The potential mechanism was explored by western blot and Co-IP assays.

Results
Downregulated CHMP5 was identified by proteomics in OA patient cartilages, which was verified in human and mouse articular cartilages. CHMP5 overexpression repressed cell apoptosis and ECM degradation in OA chondrocytes. However, silencing CHMP5 exacerbated OA chondrocyte apoptosis and ECM degradation. Furthermore, we found that the protective effect of CHMP5 against OA was involved in nuclear factor kappa B (NF-κB) signaling pathway.

Conclusions
This study demonstrated that CHMP5 repressed IL-1β-induced chondrocyte apoptosis and ECM degradation and blocked NF-κB activation. It was shown that CHMP5 might be a novel potential therapeutic target for OA in the future.
Neural Network Robust Control Based on Computed Torque for Lower Limb Exoskeleton
Authors: Han Yibo , Ma Hongtao , Wang Yapeng , Shi Di , Feng Yanggang , Li Xianzhong , Shi Yanjun , Ding Xilun , Zhang Wuxiang 
Published: April 2024

AbstractThe lower limb exoskeletons are used to assist wearers in various scenarios such as medical and industrial settings. Complex modeling errors of the exoskeleton in different application scenarios pose challenges to the robustness and stability of its control algorithm. The Radial Basis Function (RBF) neural network is used widely to compensate for modeling errors. In order to solve the problem that the current RBF neural network controllers cannot guarantee the asymptotic stability, a neural network robust control algorithm based on computed torque method is proposed in this paper, focusing on trajectory tracking. It innovatively incorporates the robust adaptive term while introducing the RBF neural network term, improving the compensation ability for modeling errors. The stability of the algorithm is proved by Lyapunov method, and the effectiveness of the robust adaptive term is verified by the simulation. Experiments wearing the exoskeleton under different walking speeds and scenarios were carried out, and the results show that the absolute value of tracking errors of the hip and knee joints of the exoskeleton are consistently less than 1.5°and 2.5°, respectively. The proposed control algorithm effectively compensates for modeling errors and exhibits high robustness.
The research trends and future prospects of nanomaterials in breast cancer
Authors: Li Yue , Li Xiaoqing , Li Aoqun , Zhu Jingyan , Lin Zhenhua , Yang Yang 
Published: April 2024

Abstract
Background
Breast cancer is the most common cause of cancer-related deaths among women globally and the most deadly illness for them. New advances in nanotechnology have led to the development of strategies intended to target breast cancer cells more precisely while causing the least amount of damage to healthy cells. We retrieved articles about nanomaterials for the diagnosis and treatment of breast cancer from the Web of Science Core Collection (WoSCC) database between 2008 and 2023. Our research aims to assess publications on the use of nanomaterials for breast cancer treatment and diagnosis to predict future research directions.

Results
A total of 457 papers on nanomaterials in breast cancer were discovered from various nations, with China being the primary source and the United States having the highest H index. The number of papers in this discipline is increasing on an annual basis. The Egyptian Knowledge Bank is an important research center in this sector. The International Journal of Nanomedicine has the most papers, and Kesharwani P is the most frequently referenced author. The most quoted article was written by Miele, Evelina of India in 2009. Topics such as drug delivery may be emerging areas of research.

Conclusion
Our findings predict that the use of nanomaterials in medication delivery will become a significant research area in the future, and provide valuable references for scholars investigating the role of nanotechnology in breast cancer.
Effects of four bolete species on ectomycorrhizae formation and development in Pinus thunbergii and Quercus acutissima
Authors: Tan Qianwen , You Lunhe , Hao Chen , Wang Jianrui , Liu Yu 
Published: April 2024

Abstract
Background
Bolete cultivation is economically and ecologically valuable. Ectomycorrhizae are advantageous for plant development and productivity. This study investigated how boletes affect the formation of Pinus thunbergii and Quercus acutissima ectomycorrhizae using greenhouse-based mycorrhizal experiments, inoculating P. thunbergii and Q. acutissima with four species of boletes (Suillus bovinus, Suillus luteus, Suillus grevillei, and Retiboletus sinensis).

Results
Three months after inoculation, morphological and molecular analyses identified S. bovinus, S. luteus, S. grevillei and R. sinensis ectomycorrhizae formation on the roots of both tree species. The mycorrhizal infection rate ranged from 40 to 55%. The host plant species determined the mycorrhiza morphology, which was independent of the bolete species. Differences in plant growth, photosynthesis, and endogenous hormone secretion primarily correlated with the host plant species. Infection with all four bolete species significantly promoted the host plants’ growth and photosynthesis rates; indole-3-acetic acid, zeatin, and gibberellic acid secretion increased, and the abscisic acid level significantly decreased. Indole-3-acetic acid was also detected in the fermentation broths of all bolete species.

Conclusions
Inoculation with bolete and subsequent mycorrhizae formation significantly altered the morphology and hormone content in the host seedlings, indicating growth promotion. These findings have practical implications for culturing pine and oak tree species.
Production of kojic acid by Aspergillus flavus OL314748 using box-Behnken statistical design and its antibacterial and anticancer applications using molecular docking technique
Authors: Mahmoud Ghada Abd-Elmonsef , Abdel Shakor Abo bakr , Kamal-Eldin Nahla A. , Zohri Abdel-Naser A. 
Published: April 2024

AbstractKojic acid is a wonderful fungal secondary metabolite that has several applications in the food, medical, and agriculture sectors. Many human diseases become resistant to normal antibiotics and normal treatments. We need to search for alternative treatment sources and understand their mode of action. Aspergillus flavus ASU45 (OL314748) was isolated from the caraway rhizosphere as a non-aflatoxin producer and identified genetically using 18S rRNA gene sequencing. After applying the Box-Behnken statistical design to maximize KA production, the production raised from 39.96 to 81.59 g/l utilizing (g/l) glucose 150, yeast extract 5, KH2PO4 1, MgSO4.7H2O 2, and medium pH 3 with a coefficient (R2) of 98.45%. Extracted KA was characterized using FTIR, XRD, and a scanning electron microscope. Crystalized KA was an effective antibacterial agent against six human pathogenic bacteria (Bacillus cereus, Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia, Serratia marcescens, and Serratia plymuthica). KA achieves high inhibition activity against Bacillus cereus, K. pneumonia, and S. plymuthica at 100 μg/ml concentration by 2.75, 2.85, and 2.85 compared with chloramphenicol which gives inhibition zones 1, 1.1, and 1.6, respectively. Crystalized KA had anticancer activity versus three types of cancer cell lines (Mcf-7, HepG2, and Huh7) and demonstrated high cytotoxic capabilities on HepG-2 cells that propose strong antitumor potent of KA versus hepatocellular carcinoma. The antibacterial and anticancer modes of action were illustrated using the molecular docking technique. Crystalized kojic acid from a biological source represented a promising microbial metabolite that could be utilized as an alternative antibacterial and anticancer agent effectively.
Genomic characterization and probiotic potential assessment of an exopolysaccharide-producing strain Pediococcus pentosaceus LL-07 isolated from fermented meat
Authors: Lu Kuan , Wang Xueya , Zhou Ying , Zhu Qiujin 
Published: April 2024

Abstract
Background
The genomic information available for Pediococcus pentosaceus is primarily derived from fermented fruits and vegetables, with less information available from fermented meat. P. pentosaceus LL-07, a strain isolated from fermented meat, has the capability of producing exopolysaccharides (EPS). To assess the probiotic attributes of P. pentosaceus LL-07, we conducted whole-genome sequencing (WGS) using the PacBio SequelIIe and Illumina MiSeq platforms, followed by in vitro experiments to explore its probiotic potential.

Results
The genome size of P. pentosaceus LL-07 is 1,782,685 bp, comprising a circular chromosome and a circular plasmid. Our investigation revealed the absence of a CRISPR/Cas system. Sugar fermentation experiments demonstrated the characteristics of carbohydrate metabolism. P. pentosaceus LL-07 contains an EPS synthesis gene cluster consisting of 13 genes, which is different from the currently known gene cluster structure. NO genes associated with hemolysis or toxin synthesis were detected. Additionally, eighty-six genes related to antibiotic resistance were identified but not present in the prophage, transposon or plasmid. In vitro experiments demonstrated that P. pentosaceus LL-07 was comparable to the reference strain P. pentosaceus ATCC25745 in terms of tolerance to artificial digestive juice and bile, autoaggregation and antioxidation, and provided corresponding genomic evidence.

Conclusion
This study confirmed the safety and probiotic properties of P. pentosaceus LL-07 via complete genome and phenotype analysis, supporting its characterization as a potential probiotic candidate.
GMOIT: a tool for effective screening of genetically modified crops
Authors: Zhou Pu , Liu Xuan , Liang Jingang , Zhao Juanli , Zhang Yuqi , Xu Dongmei , Li Xiaying , Chen Ziyan , Shi Zongyong , Gao Jianhua 
Published: April 2024

Abstract
Background
Advancement in agricultural biotechnology has resulted in increasing numbers of commercial varieties of genetically modified (GM) crops worldwide. Though several databases on GM crops are available, these databases generally focus on collecting and providing information on transgenic crops rather than on screening strategies. To overcome this, we constructed a novel tool named, Genetically Modified Organisms Identification Tool (GMOIT), designed to integrate basic and genetic information on genetic modification events and detection methods.

Results
At present, data for each element from 118 independent genetic modification events in soybean, maize, canola, and rice were included in the database. Particularly, GMOIT allows users to customize assay ranges and thus obtain the corresponding optimized screening strategies using common elements or specific locations as the detection targets with high flexibility. Using the 118 genetic modification events currently included in GMOIT as the range and algorithm selection results, a “6 + 4” protocol (six exogenous elements and four endogenous reference genes as the detection targets) covering 108 events for the four crops was established. Plasmids pGMOIT-1 and pGMOIT-2 were constructed as positive controls or calibrators in qualitative and quantitative transgene detection.

Conclusions
Our study provides a simple, practical tool for selecting, detecting, and screening strategies for a sustainable and efficient application of genetic modification.