bluuebunny/crossref_metadata_2025_split huggingface dataset:
Conclusion
Authors: McGeough Kevin M 
Published: February 2025

Abstract
The Conclusion brings together many of the themes of the book, discussing how the Ark functions as a metasymbol for different interpretive communities. It introduces references to the Ark in popular music and Christian metal, and it considers how references in these media reflect interpretations of the Ark discussed in prior chapters. It also examines pseudoarchaeological claims that the Dead Sea Scrolls hide secrets that the Ark may be buried at Qumran and discusses why these claims are not legitimate. These and other claims about the Ark sit at the intersection of pseudoscholarly beliefs, political maneuvering surrounding the Temple Mount in Jerusalem, and apocalyptic thinking in contemporary Christianity and Judaism.
The Seasons: Paradigm of Lyric Time
Authors: Stewart Susan 
Published: February 2025

Abstract
This chapter turns to seasonality as a paradigm from nature that allows poets to think through cyclical processes of change and recurrence. It differentiates lyric time from narration in order to show that, whereas narrative is bound to causal order, teleology, and techniques of explanation, the lyric moves through time while also drawing itself and its reader out of time. The intricacy of lyric invites rereading. The chapter reads poems by Clare, Shakespeare, Cowper, and Keats in order to explore poets’ utilization of seasonal growth and patterning and also posits that the human ability to notice seasonal patterns is reflected in the human ability to make poems, which have their own aural and formal patterns of rhyme, refrain, and recurrence.
The Imperceptible, or Wilderness
Authors: Stewart Susan 
Published: February 2025

Abstract
This chapter returns to Kant’s phenomena/noumena distinction and asks how the noumenal might be approached in poems. Poetry, it argues, is a liminal art, always pushing to the limits of our senses. Nature poetry addresses a vast domain of animal and organic life that is unknown to us; when we apprise nature it is only ever as a fragment. The chapter considers the perceptual paradox that the perception of detail can result in abstraction; the imperceptible can be too close, too far, or, as in poetry regarding storms reveals, too overwhelming. In closing, the study turns to read three North American seashore poems, using the ocean as an exemplar of the unknown or wilderness, before finally placing human beings, with our ability to notice, find patterns, imagine, and create within the domain of an indifferent and ever-creating nature.
Untangling host specialization in a “double dark taxa” system
Authors: Awad Jessica , Reinisch Ronja , Moser Marina , Vasilița Cristina , Krogmann Lars 
Published: February 2025

Abstract
Platygastrine wasps (Hymenoptera: Platygastridae) are parasitoids of gall midges (Diptera: Cecidomyiidae). They and their hosts are exceptionally abundant and speciose, with great relevance to agriculture and biodiversity research. Both groups are also “dark taxa,” whose species identification and ecological associations are obscured by a history of taxonomic confusion and neglect. Verified host records are few in number and limited in scope. In order to understand host specialization, more records are needed. However, rearing Cecidomyiidae is challenging, as many species require living host tissue to complete development. There is no universal rearing method for Cecidomyiidae and their parasitoids. The present work applies an exploratory approach to rearing gall midges, with the aim of obtaining accurate host associations and parasitoid identifications. We obtained 5 species of Platygastrinae from reared material, 3 of which are identified and diagnosed. Platygaster demades Walker (= Platygaster marchali Kieffer, syn. nov. = Platygaster ornata Kieffer, syn. nov.) is not host-specific, attacking Cecidomyiidae on Rosaceae worldwide, including Filipendula ulmaria. Synopeas gibberosum Buhl apparently specializes on Dasineura ulmaria (Bremi) on F. ulmaria. Synopeas rhanis (Walker) is known only from galls of D. urticae (Perris), but may attack other midge species on Urtica dioica. Amblyaspis sp. emerged from Hartigiola annulipes (Hartig) galls on Fagus sylvatica, and Synopeas sp. was associated with Mycodiplosis sp. on Rubus sp. Illustrations, DNA barcodes, and distributions are provided. We discuss challenges to understanding “double dark taxa” interactions, implications for biological control, and possible solutions for future research on these important but neglected systems.
Laticifers are present in Acalyphoideae after all: new insights from leaf anatomy with implications for the systematics and evolution of Euphorbiaceae
Authors: Mouzella Clara Prandi , Sousa Ana Angélica , Rossi Caruzo Maria Beatriz , Montero-Muñoz Iris , Strozi Alves Meira Renata Maria , Riina Ricarda 
Published: February 2025

Abstract
Euphorbiaceae is among the main angiosperm families with a high number of laticiferous species. Although many of its species remain to be studied in terms of their anatomy, chemistry, and uses, there are some of recognized economic importance due to useful secondary compounds present in the latex. Acalyphoideae, one of the three major subfamilies, has traditionally been distinguished from the rest of Euphorbiaceae by the absence of latex and laticifers. To test this long-standing assumption, we anatomically analyzed 40 species in 10 genera, representing six of the nine subclades of Acalyphoideae s.s., to examine the presence of laticifers using leaf blade and petiole sections. Laticifers were observed in all the studied species and consisted of multinucleate, elongated cells with dense cytoplasm. They were articulated and branched in Acalypha, Bia and Dalechampia; this was further confirmed by ontogenetic analyses in Acalypha accedens, A. brasiliensis and A. poiretii. Histochemical tests revealed lipids, proteins, mucilage and starch in laticifers. Our results demonstrate that laticifers are present and common in Acalyphoideae and thus more widespread in Euphorbiaceae than previously known. The scarcity of detailed anatomical studies, and the often imperceptible latex exudation of most Acalyphoideae, are probably the main reasons that have misled field botanists and systematists in the past.
Trans-catheter aortic valve implantation in a patient with membranous ventricular septal defect, sub-aortic band and double-chambered right ventricle: a case report
Authors: Mitsuhashi Hirotsugu , Yamasaki Takuma , Nakamura Ryuta , Uno Yoshihide , Tei Imun 
Published: February 2025

Abstract

Background
We report a rare case of trans-catheter aortic valve implantation in an elderly male with membranous ventricular septal defect (VSD), sub-aortic band, and severe aortic stenosis (AS). We discuss the safety and efficacy of the technique.

Case Summary
An 86-year-old male was admitted to our hospital with congestive heart failure (CHF) due to low- flow low-gradient severe AS, a membranous ventricular septal defect (VSD), a subaortic band, and a double chambered right ventricle. The patient was not deemed to be a surgical candidate because of advanced age and frailty even though surgical aortic valve replacement, VSD closure, subaortic band resection and myectomy of right ventricle would be considered as definitive treatment. Instead, we performed transcatheter aortic valve implantation and VSD orifice closure using the skirt part of the self-expanding valve (26mm Evolut Pro PlusTM) because VSD occluder is not approved and thus not available in our country. The trans-catheter procedure resulted in a reduction of the mean aortic valve pressure gradient improved from 33 to 2 mmHg and a decrease in the shunt flow (Qp/Qs) from 1.9 to 1.2. The patient’s heart failure improved, and he was discharged to home 7 days after the procedure. He remained well and had not been admitted to hospital since discharge.

Discussion
Trans-catheter aortic valve implantation using a valve skirt may be considered in a situation where a high-risk patient is inoperable and VSD closure devices are unavailable. To complete this procedure safely, meticulous pre-procedural evaluation and accurate positioning using trans-oesophageal echocardiogram are required.
Ischemic and bleeding risk after ST-elevation myocardial infarction in patients with active cancer; a nationwide study
Authors: Dafaalla Mohamed , Costa Francesco , Jia Haibo , Wijeysundera Harindra , Rashid Muhammad , Graham Michelle M , Wojakowski Wojtek , Chieffo Alaide , Mintz Gary S , Mamas Mamas A 
Published: February 2025

Abstract

Background
Treatment of patients with cancer presenting with ST-elevation myocardial infarction (STEMI) is complex given the increased risk of both thrombotic and major bleeding complications.

Methods
A nationally-linked cohort of STEMI patients between January 2005 and March 2019 were obtained from the UK Myocardial Infarction National Audit Project and the UK national Hospital Episode Statistics Admitted Patient Care registries. The primary outcomes were major bleeding and re-infarction at 1 year following admission with STEMI. Major bleeding was defined as bleeding events that require hospital admission. Re-infarction was defined as acute MI according to the 4th Universal Definition of Myocardial Infarction.

Results
A total of 322,776 STEMI indexed admissions were identified between Jan 2005 and March 2019. Of those, 7050 (2.2%) patients were diagnosed with active cancer. Cancer patients were older with more cardiovascular comorbidities. Cancer patients received invasive coronary angiography (62.2% vs 72.7%, p&lt;0.001) and PCI (58.4% vs 69.5%, p&lt;0.001) less often compared to patients without cancer and were less likely to be prescribed DAPT (85% vs 95.4%, p&lt;0.001). The incidence of major bleeding (6.5% vs 3.5%, p &lt;0.001) and re-infarction (cancer 5.7%, no cancer 5.1%, p = 0.01) were higher in cancer patients at one year. After adjustment for differences in baseline covariates, a similar risk of re-infarction (SHR 1.10, 95% CI 0.94-1.27) and a 50% increased risk of major bleeding (SHR 1.49, 95% CI 1.30-1.71) were observed in cancer patients.

Conclusion
Compared to non-cancer patients, cancer patients have a higher risk of major bleeding but not of re-infarction. Mitigating bleeding risk in STEMI patients with cancer is of paramount importance to improve outcomes.
Proximal ligation of pulmonary vein stump to prevent postoperative cerebral infarction after lobectomy
Authors: Nishimura Tomoki , Okada Satoru , Kameyama Kenji , Honda Kazuki , Nakazono Chiaki , Tokuda Ryousuke , Ishihara Shunta , Teramukai Satoshi , Inoue Masayoshi , Ueshima Yasuo 
Published: February 2025

Abstract

OBJECTIVES
We aimed to assess whether proximal ligation of the pulmonary vein stump (PVS) outside the pericardium reduces postoperative cerebral infarction (PCI) incidence after lobectomy.

METHODS
A single-institution retrospective review was conducted on 683 patients undergoing lobectomy for lung cancer, metastatic lung tumours, and benign diseases. Associations among PCI within 90 days, ligation of the PVS, and clinicopathological features were analysed. Effects of PVS ligation on PCI were assessed using multivariable regression. PVS length measured on three-dimensional computed tomographic angiography was compared between the non-ligation and ligation groups.

RESULTS
The non-ligation and ligation groups included 356 (January 2010 to March 2018) and 327 patients (April 2018 to November 2023), respectively. Seven non-ligation group patients (1.96%) underwent PCI (three in left upper lobectomy [LUL] and left lower lobectomy [LLL] and one in right lower lobectomy); no ligation group patients suffered PCI (p = 0.016). Univariable analysis showed that LUL, LLL, chronic kidney disease (CKD), heart failure, cardiovascular disease, and PVS ligation were significantly associated with PCI. Multivariable analysis demonstrated that PVS ligation (p = 0.034) was correlated with decreased PCI and LUL (p = 0.011), LLL (p = 0.010) and CKD (p = 0.004) with increased PCI. After ligation, PVS length was shortened in each lobe in a subset analysis of 109 patients.

CONCLUSIONS
Proximal ligation of the PVS outside the pericardium may be an option to prevent PCI in lobectomy. Patients with CKD and those undergoing left-sided lobectomy are at higher risk for PCI and may benefit from more intensive management.
SEARCHING FOR THE ‘MAGIC PILLS’ FOR UNMET MEDICAL NEEDS IN SCHIZOPHRENIA AND OTHER NEUROPSYCHIATRIC DISORDERS: LEARNING FROM A NOVEL DAO INHIBITOR
Authors: Da Luo -Zhong , Tseng Yufeng Jane , Lai *Wen-Sung 
Published: February 2025

Abstract
Excitatory neurotransmission mediated by N-methyl-D-aspartate receptor (NMDAR, a glutamate receptor) is primitive to and widely spread in mammalian central nervous system. It has been implicated in many fundamental functions and complex mental functions, including neuronal plasticity, learning, memory, and schizophrenia. Schizophrenia, a severe mental illness, affects 1% of population worldwide, including Taiwan. Common symptoms associated with schizophrenia include positive, negative, and cognitive symptoms. While existing agents treat positive symptoms of schizophrenia relatively well, negative and cognitive symptoms of schizophrenia have become an unmet medical need for antipsychotics development. In recent decades, increasing evidence from genetic, clinical, and pharmacological studies supports the NMDAR hypofunction hypothesis of schizophrenia. Blockade of the NMDAR can precipitate schizophrenic-like symptoms in both normal humans and animal models. Notably, several NMDA-enhancing agents, especially through glycine modulatory site (GMS) of NMDAR, resulted in a significant reduction of schizophrenia-related symptoms in patients. Particularly, D-amino acid oxidase (DAO) is now of great interest for the treatment of schizophrenia and several psychiatric disorders because its major substrate in the brain is D-serine, a co-agonist of the GMS on NMDAR. Accumulating data also provide clear evidence of increased cerebellar DAO (mRNA and enzyme activity) in schizophrenia. DAO inhibitor is one of the most attractive therapeutic targets for improving cognition and reducing negative symptoms in schizophrenia. Our inter-institutional research team has developed RS-D7 (a new chemical entity and a novel DAO inhibitor) for the treatment of negative and cognitive symptoms of schizophrenia for several years and obtained several awards. Given that NMDAR-mediated signaling pathway has been implicated in cognitive functions and that DAO inhibitors are potential therapeutic targets for enhancing activation of NMDARs, it is of great interest to investigate the effects of RS-D7 on the regulation of schizophrenia-related deficits and the underlying neural mechanisms in pharmacological (i.e., MK-801-induced) and genetic (i.e., serine racemase mutant) NMDAR hypofunction mouse models of schizophrenia. A series of cell-based assays and mouse studies was conducted and indicated efficacy, therapeutic effects, and underlying mechanism of RS-D7. Taking advantage of RS-D7 can be converted through a market drug RS-D7pro, our team further demonstrated RS-D7’ s significant therapeutic potentials in negative/cognition symptoms of schizophrenia and other neuropsychiatric disorders (e.g., multiple system atrophy) in our proof-of-concept clinical studies.
Collectively, the therapeutic potentials of DAO inhibitors (especially RS-D7) on schizophrenia and other neuropsychiatric disorders are worth further investigation.
CLINICAL AND ECONOMIC BURDEN OF MAJOR AND TREATMENT- RESISTANT DEPRESSION IN A POPULATION-BASED STUDY COHORT OF 110.000 PATIENTS
Authors: Pappa *Sofia , Shah Moulesh , Young Sophie , Anwar Tazneem , Ming Timothy 
Published: February 2025

Abstract

Background
Major depressive disorder (MDD) is a leading cause of global disability. Despite availability of effective therapies, many patients with MDD develop treatment-resistant depression (TRD).

Aims
To evaluate and compare treatment outcomes, comorbidities and healthcare resource usage and costs in patients with major depressive disorder (MDD) and treatment-resistant depression (TRD).

Methods
This was a population-based, retrospective analysis of linked primary and secondary care NHS data in the northwest London Discover-NOW dataset. This is the access platform for the longitudinal Whole Systems Integrated Care (WSIC) database, which is hosted by Imperial College Health Partners and contains linked coded primary, acute, mental health, community health, and social care records fed by over 400 provider organisations and covering more than 2.5 million patients. Eligible patients were adults who were registered with a General Practitioner in the area, had diagnostic codes for depression and had been prescribed at least one antidepressant between 2015 and 2020.

Results
A total of 110,406 patients were eligible for inclusion (101,333 [92%] with MDD and 9,073 [8%] with TRD). Mean duration of depression was 52.8 (SD 41.7) months and 70.8 (SD 37.8), respectively. Remission was recorded in 42,348 (42%) patients with MDD and 1,188 (13%) with TRD (p&lt;0.0001) while relapse was seen in 20,970 (21%) and 4,923 (54%), respectively (p&lt;0.0001) during the study period.
Patients with TRD had significantly higher risks of suicidal behaviour and comorbidities such as anxiety, asthma, and alcohol/substance misuse (all p&lt;0.0001). Mean times from diagnosis to first contact with mental health services were 38.9 [SD 33.6] months for MDD and 41.5 [SD 32.0] months for TRD (p&lt;0.0001). Individuals with TRD had considerably higher primary care and emergency attendance rates, more inpatient hospitalizations, longer hospital stays, and greater total healthcare costs than MDD patients. Demand increased with the duration of depression and the number of lines of treatment.
Primary care usage was greatest overall (TRD mean 162 [SD 96] vs. MDD 108 [SD 90] visits per patient, p&lt;0.0001; cost per patient £17,348 [SD £33,040] vs. £12,011 [£25,588], p&lt;0.0001). In secondary care, accident and emergency visits accounted for the highest use (TRD mean 5.5 [10.6] vs. MDD 3.54 [SD 6.0] visits per patient, p&lt;0.0001) while non-elective hospitalisations incurred the highest costs (mean £ 2,518 [£8,064] vs. £1,909 [SD £6,807], p&lt;0.0001).

Conclusions
The study highlights the substantial individual patient and economic burden associated with MDD and particularly TRD in both primary and secondary care settings. Findings suggest that evidence- based treatment optimisation, timely access to newer therapies and the development of stratified care pathways and specialist services could improve patients’ outcomes.
MATERNAL EXPOSURE TO AIR POLLUTANT PM2.5 INDUCES AUTISTIC-LIKE BEHAVIOR IN OFFSPRING
Authors: Yau *Sonata Suk-yu 
Published: February 2025

Abstract

Background
Autism spectrum disorder (ASD) is a devastating neurodevelopmental disorder with increasing prevalence worldwide 1. Existing evidence indicates that environmental factors (particularly in utero or during early life) can increase ASD risk 2,3. Recent meta-analysis and systemic review concluded that exposure to PM2.5 during pregnancy increases risk of ASD in newborns4.We hypothesize that maternal exposure to PM2.5 impairs hippocampal neuroplasticity to induce behavioral deficits that resembles autism-like behavior in the offspring. Aims : Determine whether maternal exposure to PM5 induces autism-like behaviors in mouse offspring, Determine whether maternal exposure to PM5 impairs hippocampal structural and synaptic plasticity in mouse offspring.

Method
Eight to ten-week old female C57 mice will be pair-housed and will be subjected to intratracheal instillation of PM2.5 with PM2.5 suspended in artificial lung fluid at the concentration of 3.5 &micro;g/&micro;l (PM2.5 group) or vehicle as control group. PM2.5 with 30 &micro;l for each mouse, every 3 days will be administered to the female mice continuously for 15 days. After the pre-exposure, a breeder male mouse (6-8 week old) will be introduced to the holding cage for a week leaving them uninterrupted to reduce stress and increase fertility rate during mating period. Intratracheal instillation will then be resumed every three day continuously throughout gestation period. Both female and male offspring at the age of 5-6 week old will be subjected to behavioral tests, biochemical assays, electrophysiology experiments.

Results
Maternal exposure to PM2.5 induced core ASD behavior, including impaired social recognition memory, increased repetitive behavior, and impairment in hippocampal dependent learning and memory performance. The behavioral deficits are concurrent with impaired dendritic branching of immature neurons and long-term potentiation in the hippocampal dentate gyrus, though there were no significant effects on altering hippocampal adult neurogenesis and neuroinflammation.

Discussion & Conclusion
The results have suggested that chronic maternal exposure to PM2.5 could induce behavioral abnormalities in adult offspring, which could be linked to impaired neuroplasticity in the hippocampus.

References
Lyall, K., et al. The Changing Epidemiology of Autism Spectrum Disorders. Annual review of public health 38, 81-102 (2017). Gronborg, T.K., Schendel, D.E. &Parner, E.T. Recurrence of autism spectrum disorders in full- and half-siblings and trends over time: a population-based cohort study.
JAMA pediatrics 167, 947-953 (2013). Hallmayer, J., et al. Genetic heritability and shared environmental factors among twin pairs with autism. Archives of general psychiatry 68, 1095-1102 (2011). Dutheil, F., et al. Autism spectrum disorder and air pollution: A systematic review and meta analysis. Environ Pollut 278, 116856 (2021).
ANTIDEPRESSANT AUGMENTATION STRATEGIES TARGETING SIGNAL TRANSDUCTION PROOF OF CONCEPT IN ANIMAL MODELS
Authors: Tzavara *Eleni T , Gorgievski Victor , Le Marois Marguerite , Belzeaux Raoul , Ibrahim El Cherif 
Published: February 2025

Abstract

Background
Antidepressant (AD) resistant depression is a major cause of disability. We have recently identified a novel AD target, the transcription factor ELK-1. ELK-1 operates inside the cell rather than the synapse, where « classical » ADs act.

Aims & Objectives
We hypothesized that an add-on strategy (classical + ELK-1 modulator) would lead to rapid AD action and/or better efficacy.

Methods
Mice were subjected to social defeat (SD, males) or vicarious social defeat (vSD, females) for 10 days and then, screened in the social interaction test (SI). Susceptible mice (SI&lt;1) were further used in two different sets of experiments. (i) To determine the augmentation effect in the delay of onset of action, mice were treated for 6 days with either desipramine (DES; 15mg/kg), fluoxetine (FLX; 10mg/kg), the ELK-1 modulator (TDE; 2 mg/kg) or in combination (DES + TDE ; FLX + TDE). (ii) To determine the augmentation effect on treatment response, mice were treated for 14 days with desipramine; then stratified for each sex into responders (R=35) and non-responders (NR=19) in the SI. NR were divided into two groups, one treated for an additional week with DES alone (n=10) and one treated with DESI+TDE (n=9). At 21 days mice were rescreened at the SI. Same design was repeated to stratify for each sex mice treated with FLX and subsequently non-responders with FLX alone or FLX+TDE.

Results
The combination of the DESI+TDE resulted in the augmented AD effect, namely (i) a faster onset of action (6 days instead of 14 days) and (ii) a greater proportion of AD-R mice treated with the combination (6/9) as compared to DESI alone (2/10). However, using the same methodoogy, this effect was not found with the SSRI FLX+TDE combination. The augmentation effect and te selectivity for DESI was similar for both sexes. The TDE effect was prevented by the mGluR5 antagonist MPEP.

Conclusion
ELK-1 modulation could represent a promising novel augmentation strategy, most likely via glutamate rigid modulation. However, this effect does not apply to all AD classes, in particular SSRIs.