bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diagnostic and Prognostic Value of DNA Repair Gene XRCC1 ARG399GLN Polymorphism in Childhood Acute Lymphoblastic Leukemia: A Single-Center Egyptian Study
Authors: Hablas Nahed M. , Mahmoud Mona M. , Qassem Shaima S. , Morad Lamia M. 
Published: June 2024

Background
Repair pathways for DNA damage are essential for repairing various forms of DNA damage. Leukemia risk may be increased by poor DNA repair. This study was conducted to better understand the significance of XRCC1Arg399 Gln polymorphisms in acute lymphoblastic leukemia (ALL) susceptibility and prognosis.

Patients and methods
Twenty children with ALL participated in the pediatric trial at Tanta University. Thirty healthy controls were of the same age and sex. Complete blood counts, bone marrow aspirates, immunophenotyping, flow cytometry, and XRCC1 genotyping by PCR were performed.

Results
The allele Gln/Gln was found in one (3.3%) of the controls and two (10%) cases had an odds ratio of 0.13 (0.27–38.1), P=0.34. Eight (40%) patients and six controls [odds ratio of 2.66 (0.75–9.4), P=0.11], had the Arg/Gln allele. Finally, 23 (76.7%) members of the control group and 10 (50%) patients carried this allele (Arg/Arg). The genotype’s prognostic value for alleles: two (20%) of patients with the allele (Arg/Arg) were refractory. Two (25%) patients died during the induction for the allele (Arg/Gln), while four (50%) patients were refractory. Last but not least, no one experienced total remission under the allele (Gln/Gln).

Conclusion
The XRCC1 polymorphism is not linked to the development of ALL; nevertheless, having mutant alleles was linked to a poorer prognosis.
Body composition analysis in children with chronic kidney disease on regular hemodialysis
Authors: Elbarky Amany , El-Kaffas Asmaa , Gamal Rasha M. , Abdelnabi Hend H. , Elrifaey Shymaa 
Published: June 2024

Background
Children with chronic kidney disease (CKD), particularly those on hemodialysis, are at high risk for protein-energy malnutrition.

Aim
In this study, we aimed to analyzed the nutritional parameters of children with CKD undergoing hemodialysis (HD) by performing a body composition analysis.

Patients and methods
This cross-sectional study included 40 children undergoing HD and 50 matched controls. All participants underwent a 3-day dietary recall, anthropometric measurements, nutritional and laboratory assessments, and finally a bioelectrical impedance analysis was conducted using the TANITA Body Composition Analyzer.

Results
Anthropometric measures, caloric and protein intake were significantly reduced in cases compared with controls. The children undergoing HD had lower body composition (fat mass, fat mass index, fat-free mass index, skeletal muscle mass, total body water%, basal metabolic rate, and phase angle) when compared with controls.

Conclusions
Children with CKD, especially those on HD, have poor nutritional status and need regular comprehensive assessment using bioelectrical impedance analysis to improve growth outcomes.
High prevalence of cagA positive Vac A s1m1 Helicobacter pylori strains isolated from patients suffering from various gastroduodenal diseases in Guwahati, Assam, India
Authors: Mahant Shweta , Singh Sarika , Dutta Sangitanjan , Sharma Nisha , Das Prasenjit , Mukhopadhyay Asish Kumar , Das Kunal , Das Rajashree 
Published: June 2024

Abstract

Aim and Background:
Genotyping of H. pylori strains was identified on formalin-fixed paraffin wax-embedded tissue (FFPE) sections and correlated with severity grades of gastric mucosal pathologies in biopsies from upper gastrointestinal (UGI) tract from Guwahati, Assam.

Materials and Methods:
In total, 168 gastric biopsies collected from patients with UGI symptoms underwent histological evaluation as per the updated Sydney system.

Result:

H. pylori-like organisms were identified with Warthin and Starry stain, and virulent genes were amplified using polymerase chain reaction (PCR) from genomic DNA extracted from FFPE sections by using QIAamp® DNA FFPE Tissue Kit. Histological examination identified H. pylori-like organisms in 100 biopsies, of which 96 were urease + ve. The prevalence of H. pylori infection was high in age groups 71–80 (88.8%) as compared to other age groups, and it was higher in females (78.9%) when compared to males. The prevalence of virulent genes in biopsies was 88.5% cagA and vacA s1m1, 31.2% iceA1, 32.2% iceA2, and 85.2% babA2. The histological parameters mononuclear cell infiltrate (P = 0.04) and atrophy (P = 0.03), showed statistically significant association with iceA2 and intestinal metaplasia with cagA (P = 0.01) vacAs1m1 (P = 0.01) and babA (P = 0.02) genotypes. Gastric erosion due to H. pylori infection and atrophy showed a significant association. A high bacterial density score was seen with the virulent genotypes.

Conclusion:
Our work reports for the first time a high prevalence (88.5%) of H. pylori cagA vacA s1m1 genotype in Guwahati, Assam. Association of gastric atrophy and intestinal metaplasia was seen with virulent genotypes. Results show the effectiveness of the FFPE kit for DNA extraction in remote areas where transportation and storage of biopsies are otherwise difficult.
Higher expression of BRCA1, and CHUK and lower expression of NFKBIA, ESR1, PIK3R1, and PPARG in HAM/TSP compared to ATLL, a diverse pathological consequence
Authors: Yaslianifard Sahar , Movahedi Monireh , Yaslianifard Somayeh , Mozhgani Sayed-Hamidreza 
Published: June 2024

ABSTRACT

Background:
HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP) and adult T-cell leukemia/lymphoma (ATLL) are both severe diseases caused by Human T-lymphotropic virus type 1 (HTLV-1) infection, while about 95% of infected cases remain asymptomatic. Genes that play a role in ATLL development are assumed to be dissimilar from the ones that are crucial factors for HAM/TSP occurrence.

Objective:
The expression of six genes including BRCA1, CHUCK, ESR1, NFKBIA, PIK3R1, and PPARG were assessed in two groups of HAM/TSP and ATLL patients. Materials and Methods: cDNA was synthesized from purified RNA, and RT-qPCR was conducted to assess the expression of the genes in two groups. Any possible correlation among the genes’ expression was also calculated. Results: BRCA1 and CHUCK expressions were higher in HAM/TSP patients in comparison with ATLL patients. However, ESR1, NFKBIA, PIK3R1, and PPARG are more expressed in ATLL cases than HAM/TSP. A significant positive correlation was observed between BRCA1 and NFKBIA in HAM/TSP group. In addition, a significant negative correlation between PIK3R1 and PPARG in HAM/TSP and between ESR1 and NFKBIA in the ATLL group was obtained.

Conclusion:
HAM/TSP or ATLL stem from a disturbance in the expression of diverse genes and these dissimilarities should be discovered to reach a better understanding of disease treatment as well as screening and assessing the asymptomatic carriers’ condition for developing severe disease.
Variations in platelet indices in patients with diabetes mellitus and hypertension: A case-control study from a tertiary hospital in North Bengal
Authors: Roy Poulami , Deb Novonil , Lahiri Pratibha Rao , Goswami Bidyut Krishna 
Published: June 2024

ABSTRACT

Background:
Platelet indices (PI) have emerged as a useful tool for identifying platelet dysfunction and cardiovascular risk in various diseases, including diabetes mellitus (DM) and hypertension. This study aimed to investigate the variation of PI in patients with DM and hypertension in the northern part of West Bengal.

Materials and Methods:
A case-control study was conducted, which included 1096 participants, comprising 264 healthy controls, 472 individuals with DM, 220 individuals with hypertension, and 140 individuals with both hypertension and DM. PI were measured using automated hematology analyzers, and statistical analysis was performed to determine the differences in PI among the different groups.

Result:
The study results revealed that PI were significantly higher in individuals with DM and hypertension compared to healthy controls. The mean platelet volume (MPV), platelet distribution width (PDW), and platelet large cell ratio (P-LCR) were significantly higher in patients suffering from DM, hypertension and both when compared to healthy controls.

Conclusion:
The findings of this study suggest that PI can be used as a reliable and cost-effective tool for identifying platelet dysfunction and cardiovascular risk in individuals with DM and hypertension. PI can serve as a useful collaborative tool for identifying individuals at high risk of cardiovascular events in these conditions.
Assessment of cyclin D1 activity in tumors and tumor budding and its prognostic significance in tumor budding in colorectal carcinomas
Authors: Coban Ganime , Arici Dilek S. , Tekden Busra C. , Yildiz Pelin , Kiran Tugce , Toluk Özlem , Turk Haci M. , Şahin Nurhan 
Published: June 2024

ABSTRACT

Background:
The most important prognostic parameter is staging in colorectal cancers, and tumor budding (TB) is among the independent prognostic parameters that are increasing in significance. Cyclin D1 expression has been associated with poor prognosis as a marker of various tumors.

Aims and Objectives:
In this study, the aim was to determine the activity of cyclin D1 in colon adenocarcinomas, tumors, and tumor buds and to compare the results with prognostic parameters.

Materials and Methods:
This study included 167 patients who were operated on for colorectal tumors. In subjects, tumor budding was evaluated on hematoxylin and eosin-stained slides, and cyclin D1 was applied immunohistochemically. The cyclin D1 intensity of staining was studied in both tumors and TB and its correlation with prognostic parameters in TB was examined.

Results:
Lymph node (LN) metastasis was present in 93 (55.7%) of the cases, and distant metastasis in 35 (21%) cases. Tumor budding was present in 152 (91%) of the cases, and high-grade TB was detected in 55 (36.2%). The incidence of TB was higher in patients with LN metastasis (P = 0.02) and in patients with Stages 3 and 4 (P = 0.07). The intensity of cyclin D1 in intermediate and high-grade TB staining was higher. Cyclin D1 staining was more intense in patients with LN metastases and distant metastases as we determined, but it was not statistically significant.

Conclusion:
Thus, based on our study findings, the increased expression of cyclin D1, which is more concentrated in TB than tumors, may indicate a poor prognosis. In contrast, we found no statistically significant correlation between cyclin D activity and prognostic parameters in TB.
The immunohistochemical expression of c-MET and RON in lung adenocarcinoma with clinicopathologic correlation
Authors: Kim Young-Ah , Sung Woo Jung 
Published: June 2024

ABSTRACT

Background:
Mesenchymal epidermal transition (MET) and receptor originating from nantes (RON) are transmembrane tyrosine kinase receptors. Both are members of a proto-oncogene family and thus play a role in the pathogenesis of various cancers and acquired resistance to kinase inhibitors in lung cancer.

Aims:
The aim of this study was to investigate the immunohistochemical expression of c-MET and RON in lung adenocarcinoma and its clinicopathologic correlation.

Settings and Design:
Retrospective study.

Materials and Methods:
The immunohistochemical c-MET and RON expression in specimens obtained from lung adenocarcinoma (n = 175) and associated clinicopathologic parameters were evaluated.

Statistical Analysis Used:
The correlation between c-MET and RON expression was analyzed by Chi-square test. A Cox proportional hazards model and Kaplan-Meier curve analysis were used to evaluate the risk factors and prognosis.

Results:
High expression of the c-MET protein showed a strong correlation with that of RON (P = 0.013, kappa = 0.183). Five-year survival and recurrence-free 5-year survival were not associated with high expression of c-MET or RON. High c-MET expression was significantly associated with age older than 60 years (P = 0.000), tumor differentiation (P = 0.009), lymphovascular invasion (P = 0.016), and pleural invasion (P = 0.005). High RON expression was associated with a solid growth pattern (P = 0.001) and pleural invasion (P = 0.002).

Conclusions:
The results point to the potential of immunohistochemical expression of c-MET and RON as useful prognostic markers of unfavorable histopathologic features in lung adenocarcinoma.
Renal allograft failure resulting from diffuse cortical necrosis
Authors: Fernandes Gwendolyn , Patil Manali , Harinkhede Nupur , Khumanthem Gloria , Rojekar Amey 
Published: June 2024

ABSTRACT
Cortical necrosis of the renal allograft is an extremely rare but serious complication of renal transplantation and can lead to graft failure. Few cases have been reported in the literature to date. We present a rare case of a 28-year-old woman with chronic kidney disease for the past four years who was on biweekly hemodialysis and referred to our tertiary care center for a living-related renal transplant. The patient developed tacrolimus toxicity on the second day post-transplant. The renal biopsy performed on third day after transplant showed ischemic acute tubular injury. C4d and donor-specific antibodies were negative. On day 12 post-transplant, USG Doppler of the renal allograft showed thrombosis of the renal vein, and an open wedge biopsy was performed that showed extensive renal cortical necrosis. A month and half later, the patient developed pus collection anterior to the transplanted kidney and a fistula from the perinephric space up to the skin with persistent pus discharge. Two months later, a graft nephrectomy was performed, and diffuse cortical necrosis of renal allograft with a thick band of exudate on the capsule was diagnosed on histopathology. Ureteral necrosis and thrombosis of both renal artery and renal vein were also seen. In this case, the etiology of graft cortical necrosis is multifactorial and includes acute ischemia, urologic complications, and sepsis. To prevent graft failures, it is critical to differentiate thrombosis caused by surgical complications from hyperacute or acute humoral rejection.
An unusual case of scalp ulceration
Authors: Gupta Sonali , Singh Poonam , Asati Dinesh P. , Gowda Shreya K. 
Published: June 2024

ABSTRACT
Granulomatosis with polyangiitis, a form of necrotizing vasculitis can present as a limited variant with the involvement of a single organ system. The superficial protracted form can present as face and scalp ulceration for chronic periods of time. Diagnosis can be established histopathologically by examining the involvement of blood vessels. Here, we present a case of scalp ulceration that was diagnosed as a limited variant and started on steroids and immunosuppressants with excellent response.
Karyomegalic interstitial nephritis, a fascinating histopathologic entity for pathologists: Be watchful of the FAN1 gene mutations
Authors: Walia Gurpreet K. , Vankalakunti Mahesha , Akal Ramanjit S. , Jha Vijoy K. 
Published: June 2024

ABSTRACT
Karyomegalic interstitial nephritis (KIN) is an uncommon autosomal recessive disease, which is characterized by enlarged and hyperchromatic nuclei of the renal tubular epithelial cells. It is associated with mutations in Fanconi anemia-associated nuclease 1 gene, which is responsible for DNA repair, and these pathogenic mutations are responsible for progressive renal failure in young adults. We present a case of a 29-year-old female with end-stage renal disease who had a family history of early-onset renal failure in two of the siblings. Her elder sister with chronic kidney disease stage III was advised to be biopsied to look for any familial causes. Stained sections of renal biopsy revealed normal glomeruli. Tubular epithelial cells showed nuclear changes focally like hyperchromasia, karyomegaly, and anisonucleosis. On direct immunofluorescence, all glomeruli were negative for deposits with all antisera. Based on these findings, a final opinion of KIN was given.
Bing–Neel syndrome: A rare complication of waldenstrom macroglobulinemia
Authors: Kudva Ranjini , Monappa Vidya , Menon Girish 
Published: June 2024

ABSTRACT
Central nervous system involvement is a rare complication of Waldenstrom macroglobulinemia (WM) accounting for 1–2% cases. This syndrome is called Bing–Neel syndrome (BNS) after the people who first described it in 1936. Although WM has a good prognosis, with the onset of this syndrome, most patients fare poorly with a high mortality rate. A 77/male, a known case of WM, presented with left upper limb weakness of 2 weeks duration. Magnetic resonance imaging brain showed diffuse pachymeningeal thickening along bilateral frontoparietal convexity. Biopsy showed lymphoplasmacytic lymphoma favoring a diagnosis of BNS. The patient was started on chemotherapy (rituximab + ibrutinib). At 3 months following diagnosis, the patient was admitted with Gram-negative septicemic shock and failed to recover from it. BNS is a rare complication of WM, associated with poor prognosis and an aggressive clinical course. It can occur during the course of treatment of WM, as was seen in this case. Accurate diagnosis with appropriate treatment plays a crucial role in patient management.
Association between vedolizumab and risk of clostridium difficile infection in patients with ulcerative colitis: A systematic review and meta-analysis
Authors: Alghamdi Maha , Alyousfi Dareen , Mukhtar Mariam S. , Mosli Mahmoud 
Published: June 2024

Abstract

Background:
The medical treatment of ulcerative colitis (UC) includes the use of biological agents such as vedolizumab, a gut-selective alpha4beta7 (ɑ4β7) antagonist. The mechanism of action of vedolizumab involves interfering with leukocyte trafficking into the gut vasculature, which halts inflammation. Due to this mechanism of action, concerns have arisen regarding an increased risk of gut infections, specifically, clostridium difficile infection (CDI). The aim is to provide clarity regarding the association between the use of vedolizumab as a therapy for ulcerative colitis and the risk of developing CDI.

Methods:
A systematic literature review was conducted, starting with the scoping search, followed by backward snowballing parallel with keyword-based search to identify related articles. A quality assessment was conducted on the initially selected articles and excluded low-quality papers.

Results:
Pooled analyses indicated that there was no significant association between the use of vedolizumab and the risk of developing CDI (effect size = 0.03 [-0.02, 0.07]).

Conclusions:
Vedolizumab does not increase the risk of CDI in patients with UC. Further studies are needed to confirm these findings.
A benign masquerade of eyelid malignancy: Discoid lupus erythematosus
Authors: Durairaj Arjun , Arora Rohan , Menon Vikas , Subramanian Krishnakumar 
Published: June 2024

Abstract
A 50-year-old female presented with redness and itching over her right lower eyelid for 6 months. She had a diffusely hyperpigmented lesion with a purplish hue over her right lower eyelid. There was diffuse loss of eyelashes and obliteration of meibomian gland openings along the lesion. The ocular examination was otherwise unremarkable. In view of the clinical features, the possibility of squamous cell carcinoma was considered, and an incisional biopsy was performed. Histopathology was suggestive of discoid lupus erythematosus. The patient was treated with oral hydroxychloroquine sulfate for 6 months. The lesion regressed, and no new lesions developed over a 2-year follow-up. This report showcases how a benign lesion can rarely present with clinical features classically associated with a malignant pathology and highlights the importance of histopathological evaluation for proper diagnosis.
On Higher Order Jacobsthal Hyper Complex Numbers
Authors: Özimamoğlu Hayrullah 
Published: June 2024

In this work, we define a new class of hyper complex numbers whose components are higher order Jacobsthal numbers, and call such numbers as the higher order Jacobsthal $ 2^{s} $-ions. We obtain some algebraic properties of the higher order Jacobsthal $ 2^{s} $-ions such as recurrence relation, Binet-like formula, generating function, exponential generating function, Vajda's identity, Catalan’s identity, Cassini’s identity and d’Ocagne’s identity. Morever we derive the matrix representation of the higher order Jacobsthal $ 2^{s} $-ions, and so prove Cassini's identity as a further type.
Peran Badan Pengawas Pemilihan Umum dalam Pemberantasan Politik Uang Di Kabupaten Minahasa
Authors: Paseki Dicky Janeman , Pinasang Boby , Taroreh Hironimus 
Published: June 2024

The aim of the research is limited to legal efforts that can be carried out by the General Election Supervisory Agency to eradicate money politics in Minahasa Regency. Normative legal research methods utilize case approaches and legislation. Literature study in the form of legal materials as well as interviews and observations of data collection tools. Research results show that Bawaslu has the task of preventing and taking action against elections, as well as election process disputes in accordance with Article 93 letter b of Law Number 7 of 2017 concerning Elections. Bawaslu is even closer if it is said to be active in the process of upholding election justice related to preventing money politics.
EFECTO DE CUATRO FERTILIZANTES FOLIARES, PARA DETERMINAR LA PRODUCCIÓN Y CALIDAD NUTRITIVA DEL PASTO Brachiaria brizantha cv. PAIAGUÁS
Authors: Polo Edgar , Urieta Sebastián , Moreno Yaliska 
Published: June 2024

En un diseño de bloques completos al azar con tres repeticiones se evalúo el efecto que tiene el uso de cuatro tipos de fertilizantes foliares (12-8-16+3, 16-32-16, 20-20-20 y la urea) en época seca sobre la calidad nutricional y rendimiento del pasto Brachiaria brizantha cv Paiaguás. Se realizaron cuatro cortes con intervalos de 30 días. Hubo efecto significativo (P < 0.05) sobre la altura de las plantas durante el periodo estudiado como también en los diferentes tratamientos foliares que se aplicaron en el trabajo. La altura de las plantas fue decreciendo a medida que avanzaba la época seca. La mayor respuesta a fertilización foliar se reflejó en los tratamientos 16-32-16, Urea, Testigo y 12-8-16+3 con alturas de plantas promedio de 39.29, 38.52 y 38.38 y 37.90 centímetros respectivamente. La cobertura en los cortes a medida que avanzaba el verano fue decreciendo, obteniéndose el mayor porcentaje con el tratamiento foliar Urea. El análisis de varianza para la variable rendimiento de materia seca indicó que no existió diferencias significativas (P >0.05) entre los fertilizantes foliares estudiados. Sin embargo, si se presentaron diferencias significativas entre los cortes realizados en el estudio (P < 0.05). La proteína cruda fue decreciendo, a lo largo de los cortes realizados. El tratamiento foliar a base de urea fue el que mayor contenido de proteína cruda (8.82%) mostro en la investigación. Los contenidos de Fibra cruda que se obtuvieron en los fertilizantes foliares son considerados como porcentajes regulares. Los fertilizantes foliares presentaron niveles bajos en cuanto a porcentajes de calcio con porcentajes entre 0.21 a 0.23%.
Hätte, hätte, Wertschöpfungskette
Authors: Vetter Bernhard 
Published: June 2024

Frankfurt a.M. Premiere bei den DLG-Feldtagen 2024: Der Tag der Wertschöpfungsketten will Landwirtinnen und Landwirte mit Akteuren des nachgelagerten Bereichs vernetzen. Der Fokus liegt auf Pflanzenproteinen, Weizen und Bio.