bluuebunny/crossref_metadata_2025_split huggingface dataset:
Proteomic study of amyloid beta (25–35) peptide exposure to neuronal cells: Impact on APE1/Ref‐1's protein–protein interaction
Authors: Mantha Anil K. , Dhiman Monisha , Taglialatela Giulio , Perez‐Polo Regino J. , Mitra Sankar 
Published: April 2012

AbstractThe genotoxic, extracellular accumulation of amyloid β (Aβ) protein and subsequent neuronal cell death are associated with Alzheimer's disease (AD). APE1/Ref‐1, the predominant apurinic/apyrimidinic (AP) endonuclease and essential in eukaryotic cells, plays a central role in the base excision repair (BER) pathway for repairing oxidized and alkylated bases and single‐strand breaks (SSBs) in DNA. APE1/Ref‐1 is also involved in the redox activation of several trans‐acting factors (TFs) in various cell types, but little is known about its role in neuronal functions. There is emerging evidence for APE1/Ref‐1's role in neuronal cells vulnerable in AD and other neurodegenerative disorders, as reflected in its nuclear accumulation in AD brains. An increase in APE1/Ref‐1 has been shown to enhance neuronal survival after oxidative stress. To address whether APE1/Ref‐1 level or its association with other proteins is responsible for this protective effect, we used 2‐D proteomic analyses and identified cytoskeleton elements (i.e., tropomodulin 3, tropomyosin alpha‐3 chain), enzymes involved in energy metabolism (i.e., pyruvate kinase M2, N‐acetyl transferase, sulfotransferase 1c), proteins involved in stress response (i.e., leucine‐rich and death domain, anti‐NGF30), and heterogeneous nuclear ribonucleoprotien‐H (hnRNP‐H) as being associated with APE1/Ref‐1 in Aβ(25–35)‐treated rat pheochromocytoma PC12 and human neuroblastoma SH‐SY5Y cell lines, two common neuronal precursor lines used in Aβ neurotoxicity studies. Because the levels of some of these proteins are affected in the brains of AD patients, our study suggests a neuroprotective role for APE1/Ref‐1 via its association with those proteins and modulating their cellular functions during Aβ‐mediated neurotoxicity. © 2012 Wiley Periodicals, Inc.
Investigation of the susceptibility of acid‐deamidated wheat gluten to in vitro enzymatic hydrolysis using Raman spectra and free amino acid analysis
Authors: Liao Lan , Wang Qin , Zhao Mou‐ming 
Published: April 2012

AbstractBACKGROUND: The number and surface nature of amino acids (AAs) in substrate proteins available to hydrolytic enzymes are critical. Among them, the micro‐environmental properties of specific AAs in substrates before hydrolysis would probably dominate the susceptibility of substrates to enzymatic hydrolysis. Fundamental knowledge concerning this regard is lacking. The objective of this work was to investigate the relationship between the exposure level of AAs in acid‐deamidated wheat gluten and their susceptibilities to in vitro enzymatic hydrolysis by pancreatin through both high‐performance liquid chromatography and Raman spectra. Wheat gluten deamidated with HCl (HDWG), citric acid (CDWG), succinic acid (SDWG) and acetic acid (ADWG) at the same degree of deamidation under the same heat treatment were chosen as the substrates. Substrate characterisations including degree of hydrolysis, surface hydrophobicity and structural characteristics before hydrolysis, together with analysis of free AAs of the corresponding hydrolysates during hydrolysis, were investigated.RESULTS: Hydrolysates from SDWG had the highest value for the degree of hydrolysis. The susceptibility of CDWG to pancreatin hydrolysis was the lowest, lower than native wheat gluten (CK) after the initial 36 h. Compared with free AAs, the mole increase profiles of CK, Arg production levelled off in HDWG after 12 h whereas it was inhibited in ADWG. For SDWG, Arg release was dramatically inhibited after 12 h and was replaced by Trp. Investigations using Raman spectra of the micro‐environment of Cys, Trp, Tyr and His and the mole increase trend of them indicated that the exposure level of these amino acids in substrates was positively related to their susceptibilities to pancreatin hydrolysis especially after 24 h of hydrolysis.CONCLUSION: Deamidation by four acids has a distinct influence on the structural characteristics of wheat gluten substrates. Although the substrates were selected at the same level of deamidation by the same heat treatment, their resultant conformational differences significantly influenced the exposure level of amino acids for binding to enzymes and the susceptibility of substrates to in vitro enzymatic hydrolysis. Therefore, it had an influence on changing enzyme cutting sites of pancreatin. This information will provide a better understanding of specific behaviour of AAs in wheat gluten during enzymatic hydrolysis from a new perspective. Copyright © 2012 Society of Chemical Industry
Impact of organic and conventional carrots on intestinal and peripheral immunity
Authors: Roselli Marianna , Finamore Alberto , Brasili Elisa , Capuani Giorgio , Kristensen Hanne L , Micheloni Cristina , Mengheri Elena 
Published: April 2012

AbstractBACKGROUND: Studies on health effects of organic (ORG) products are still limited and often contradictory. We have investigated the impact of ORG and conventional (CV) carrots from two consecutive harvest years on mouse peripheral and intestinal immunity.RESULTS: Danish carrots (Bolero variety) were grown in three ORG (O1, O2 and O3) and one CV cropping system (D‐CV). Italian carrots (Maestro and Excelso varieties) were grown in one ORG and one CV field for each variety. Immune phenotypes of blood, spleen and intestinal lymphocytes, and cytokine serum levels were analyzed in mice fed the different carrots for 30 days. Principal component analysis (PCA) was performed in mice fed the Danish carrots. The consumption of the ‘more organic’ O2 and O3 carrots induced some changes in lymphocyte populations, including an increase in regulatory T cells. In Italian carrots more differences between ORG and CV were observed in the first as compared to the second year. No relevant differences were observed in cytokine secretion. PCA showed a clear separation among mice fed the O1, O2, O3 and D‐CV carrots.CONCLUSIONS: Although a great variability was observed between the two years, an immune stimulation was found after the ORG carrot consumption. Copyright © 2012 Society of Chemical Industry
Significance of the development of the inner ear third window effect after endolymphatic sac surgery in Ménière disease patients
Authors: Kim Sung Huhn , Ko Seung Hyun , Ahn Sang Hyun , Hong Jae Min , Lee Won‐Sang 
Published: April 2012

AbstractObjectives/Hypothesis:The goal of this study was to identify the clinical significance of the low‐frequency air–bone gap (LFABG) that often develops after endolymphatic sac surgery.Study Design:Prospective study.Methods:Sixteen patients who had been diagnosed with definite Ménière disease and underwent endolymphatic sac surgery were studied. The surgical outcome was evaluated based on the 1995 guidelines of the American Academy of Otolaryngology–Head and Neck Surgery. The number of patients who developed LFABGs (a mean air–bone gap >10 dB HL at 250, 500, and 1,000 Hz) after surgery was determined, and the significance of the LFABGs was evaluated by analyzing their relation with the surgical outcome.Results:The vertigo spells of nine patients were completely controlled (class A). The number of vertigo spells was reduced by 60% to 99% in 6 patients (class B) and by 20% to 59% in 1 patient (class C). Postoperative LFABGs were observed in 13 patients. The mean LFABG of the patients in class A was significantly larger than that of the patients in classes B and C (25.0 ± 7.6 dB nHL in class A vs. 10.0. ± 7.5 dB nHL in class B and C; P = .005).Conclusions:Based on the data of the current study, we conjectured that the correlation of large LFABGs with excellent vertigo control in this study may be due to a third window phenomenon related to bony decompression of the endolymphatic sac and duct, and may serve as a favorable prognostic marker.
Accuracy of the current TNM classification in predicting survival in patients with sinonasal mucosal melanoma
Authors: Koivunen Petri , Bäck Leif , Pukkila Matti , Laranne Jussi , Kinnunen Ilpo , Grénman Reidar , Mäkitie Antti A. 
Published: April 2012

AbstractObjectives/Hypothesis:The first International Union Against Cancer (UICC) TNM classification for aerodigestive malignant mucosal melanoma was not published until 2009, and since then, only a few studies have evaluated the accuracy of this staging system. Our aim was to evaluate the accuracy of this UICC staging system for sinonasal malignant mucosal melanoma (SMMM) in a nationwide survey.Study Design:Retrospective, population‐based, multicenter study.Methods:The hospital surgical and discharge registries were used to identify the patients. A database including demographic and clinicopathologic variable was created.Results:Altogether, 50 SMMM patients diagnosed in Finland from 1990 to 2004 were evaluated. Three‐ and 5‐year overall survival rates were 44% and 27%, respectively. Significant differences in overall survival according to T classification (P = .028, log rank) and stage (P = .02, log rank) were found. Tumor extension to the sphenoid sinus had a significant negative impact on survival (n = 11, P = .03, log rank).Conclusions:The current UICC staging system for mucosal melanoma provides a useful format for staging SMMMs in clinical settings. Laryngoscope, 2012
Narrow band imaging is a new technique in visualization of recurrent respiratory papillomatosis
Authors: Tjon Pian Gi Robin E. A. , Halmos Gyorgy B. , van Hemel Bettien M. , van den Heuvel Edwin R. , van der Laan Bernard F. A. M. , Plaat Boudewijn E. C. , Dikkers Frederik G. 
Published: April 2012

AbstractObjectives/Hypothesis:Recurrent respiratory papillomatosis (RRP) is a rare, benign, wart‐like disease for which no curative treatment exists. The goal of treatment is total surgical removal of the epithelial lesions to keep the airway open and the voice sufficient. Therefore, it is essential to visualize all papillomatous lesions. The present study aims to evaluate the sensitivity of additional use of narrow band imaging (NBI) in detecting RRP during microlaryngoscopy.Study Design:Prospective study.Methods:Between January 2011 and July 2011, patients with RRP underwent systematic inspection during microlaryngoscopy using conventional white light (WL) immediately followed by inspection with NBI. Consensus was achieved about the number of lesions and number of RRP suspect lesions. All lesions were subsequently excised and sent for histopathological examination.Results:Eighty‐six excisional biopsies were taken in 24 microlaryngoscopies performed in 14 RRP patients. Eleven out of the 13 additional biopsies taken, induced by the second inspection with NBI, proved to be papillomata after histopathological examination. The sensitivity increased from 80% with WL up to 97% with WL + NBI (P < .01), whereas the specificity remained poor (32% and 28%, respectively).Conclusions:NBI is an additional diagnostic tool in increasing the sensitivity of visualizing papillomata during microlaryngoscopy. Laryngoscope, 2012
MR‐guided laser‐induced thermal therapy (LITT) for recurrent glioblastomas
Authors: Carpentier Alexandre , Chauvet Dorian , Reina Vincent , Beccaria Kevin , Leclerq Delphine , McNichols Roger J. , Gowda Ashok , Cornu Philippe , Delattre Jean‐Yves 
Published: April 2012

AbstractBackground and objectiveLaser‐induced thermal therapy (LITT), coupled with magnetic resonance thermal imaging (MRTI) guidance, provides a minimally invasive and safe approach to treat brain tumors, especially metastases. We report here our experience using this treatment for recurrent glioblastomas.Materials and MethodsFour patients, from 40 to 58 years old, were diagnosed with glioblastoma. After total resection, chemotherapy and radiation therapy, recurrence occurred. As each was ineligible for a second surgery, LITT was proposed as salvage therapy. Under stereotactic guidance, a fiberoptic applicator was inserted within the tumor LITT was performed under continuous MRTI. Real‐time feedback control based on MRTI was employed to assess the quality of local tissue destruction and to prevent unwanted damage to nearby structures.ResultsThe procedure was well tolerated with no peroperative neurological deterioration. In the short‐term follow‐up, one transient supplementary motor area syndrome, one epileptic seizure, and one cerebrospinal fluid leakage occurred. All were successfully managed. Post‐procedure MRI showed no complication, satisfying treatment volume, and a decrease in size of the treated tumor. For all patients, recurrence was observed with a mean/median progression free survival of 37/30 days. Mean/median overall survival after LITT was 10.5/10 months.ConclusionFocal tumor control was performed safely using minimally invasive LITT with real‐time MRTI control. LITT could be considered as salvage therapy for high‐grade recurrent gliomas if a 1‐day treatment is considered useful for a few weeks gain in survival. Larger experience will be required to define indications for such infiltrative disease and accurately determine a potentially significant survival gain in good neurological condition associated with this 1‐day procedure. Lasers Surg. Med. 44: 361–368, 2012. © 2012 Wiley Periodicals, Inc.
Biocompatible Zwitterionic Sulfobetaine Copolymer‐Coated Mesoporous Silica Nanoparticles for Temperature‐Responsive Drug Release
Authors: Sun Jiao‐Tong , Yu Zhi‐Qiang , Hong Chun‐Yan , Pan Cai‐Yuan 
Published: April 2012

AbstractA novel nanocontainer, which could regulate the release of payloads, has been successfully fabricated by attaching zwitterionic sulfobetaine copolymer onto the mesoporous silica nanoparticles (MSNs). RAFT polymerization is employed to prepare the hybrid poly(2‐(dimethylamino)ethyl methacrylate)‐coated MSNs (MSN‐PDMAEMA). Subsequently, the tertiary amine groups in PDMAEMA are quaternized with 1,3‐propanesultone to get poly(DMAEMA‐co‐3‐dimethyl(methacryloyloxyethyl)ammonium propanesulfonate)‐coated MSNs [MSN‐Poly(DMAEMA‐co‐DMAPS)]. The zwitterionic PDMAPS component endows the nanocarrier with biocompatibility, and the PDMAEMA component makes the copolymer shell temperature‐responsive. Controlled release of loaded rhodamine B has been achieved in the saline solutions.
Preparation of CO2/N2‐Triggered Reversibly Coagulatable and Redispersible Polyacrylate Latexes by Emulsion Polymerization Using a Polymeric Surfactant
Authors: Zhang Qi , Yu Guoqiang , Wang Wen‐Jun , Li Bo‐Geng , Zhu Shiping 
Published: April 2012

AbstractWe report here a novel approach for making reversibly coagulatable and redispersible polyacrylate latexes by emulsion (co)polymerization of methyl methacrylate (MMA) using a polymeric surfactant, poly(2‐(dimethylamino)ethyl methacrylate)10‐block‐poly(methyl methacrylate)14. The surfactant was protonated with HCl prior to use. The resulted PMMA latexes were readily coagulated with trace amount of caustic soda. The coagulated latex particles, after washing with deionized water, could be redispersed into fresh water to form stable latexes again by CO2 bubbling with ultrasonication. The recovered latexes could then be coagulated by N2 bubbling with gentle heating. These coagulation and redispersion processes were repeatable by the CO2/N2 bubbling.
Multiple 3D inversion recovery imaging for volume T1 mapping of the heart
Authors: Coniglio A. , Di Renzi P. , Vilches Freixas G. , Della Longa G. , Santarelli A. , Capparella R. , Nardiello B. , Loiudice C. , Bianchi S. , D'Arienzo M. , Begnozzi L. 
Published: April 2012

AbstractIn this article, a three‐dimensional inversion recovery sequence was optimized with the aim of generating in vivo volume T1 maps of the heart using a 1.5‐T MR system. Acquisitions were performed before and after gadolinium diethylenetriamine penta‐acetic acid (Gd‐DTPA) administration in one patient with hypertrophic cardiomyopathy and in two healthy volunteers. Data were acquired with a multishot fast field echo readout using both ECG and respiratory triggers. A dedicated phantom, composed of four solutions with different T1 values, was positioned on the subjects' thoracic region to perform patient‐specific calibration. Pixel based T1 maps were calculated with a custom Matlab® code. Phantom measurements showed a good accuracy of the technique and in vivo T1 estimation of liver, skeletal muscle, myocardium, and blood resulted in good agreement with values reported in the literature. Multiple three‐dimensional inversion recovery technique is a feasible and accurate method to perform T1 volume mapping. Magn Reson Med, 2013. © 2012 Wiley Periodicals, Inc.
Modeling dispersion in arterial spin labeling: Validation using dynamic angiographic measurements
Authors: Chappell Michael A. , Woolrich Mark W. , Kazan Samira , Jezzard Peter , Payne Stephen J. , MacIntosh Bradley J. 
Published: April 2012

AbstractA major assumption in arterial spin labeling (ASL) MRI perfusion quantification is the time course of the signal on arrival in the capillary network. The normally assumed square label profile is not preserved during transit of the label through the vasculature. This change in profile can be attributed to a number of effects collectively denoted as dispersion. A number of models for this effect have been proposed, but they have been difficult to validate. In this study ASL data acquired whilst the label was still within larger arteries was used to compare models of label dispersion. Models were fit using a probabilistic algorithm and evaluated according to their ability to fit the data. Data from an elderly population were considered including both healthy controls and patients with a variety of vascular disease. The authors conclude that modeling ASL dispersion using a convolution of the ideal ASL label profile with a dispersion kernel is most appropriate, where the kernel itself takes the form of a gamma distribution. This model provided a best fit to the data considered, was consistent with the measured flow profile in arteries and was sufficiently mathematically simple to make it practical for ASL tissue perfusion quantification. Magn Reson Med, 2013. © 2012 Wiley Periodicals, Inc.
Use of capillary blood to diagnose hereditary spherocytosis
Authors: Crisp Renée L. , Solari Liliana , Gammella Daniel , Schvartzman Gabriel A. , Rapetti María Cristina , Donato Hugo 
Published: April 2012

AbstractWe studied 31 children with hemolytic anemia, or with positive family history for hereditary spherocytosis (HS), to assess the reliability of capillary blood samples for the diagnosis. HS was diagnosed in 20 patients. Cryohemolysis (CH) was positive in 94% and eosin‐5′‐maleimide flow cytometry in 90% of them, whereas flow cytometric osmotic fragility was positive in 94%. Capillary blood sampling showed to be useful for the diagnosis. Simultaneous use of these three tests allows confirming diagnosis in 100% of patients. The use of very small blood volumes (300 µl) allows an earlier diagnosis in neonates and small infants. Pediatr Blood Cancer 2012; 59: 1299–1301. © 2012 Wiley Periodicals, Inc.
Mechanical, thermal, and rheological properties of graphene‐based polypropylene nanocomposites prepared by melt mixing
Authors: El Achaby Mounir , Arrakhiz Fatima‐Ezzahra , Vaudreuil Sébastien , el Kacem Qaiss Abou , Bousmina Mostapha , Fassi‐Fehri Omar 
Published: April 2012

AbstractIn this article, we describe the fabrication by melt mixing of graphene‐polypropylene nanocomposites and present the effect of graphene addition on some selected properties of polypropylene (PP). The graphene nanosheets (GNs) used as nano‐reinforcing agents were obtained through chemical reduction of graphene oxide by hydrazine hydrate. GNs were characterized and successfully dispersed into PP matrix to produce PP/GNs nanocomposites. The effects of GNs content on thermal, mechanical, and rheological properties were reported, and the obtained results were discussed in terms of morphology and state of dispersion and distribution of the GNs within the polymer matrix. Characterization by scanning electron microscopy and X‐ray diffraction of the nanocomposites has shown a relatively good dispersion of GNs in the polymer matrix, with the presence of only few aggregates. Increasing GNs content resulted in a significant increase in both mechanical and thermal properties with only few percent of GNs loading. Rheological behavior of the PP/GNs nanocomposites showed a Maxwellian‐like behavior for low GNs concentrations and a viscoelastic solid‐like behavior for GNs content exceeding the concentration of the percolation threshold. POLYM. COMPOS., 2012. © 2012 Society of Plastics Engineers
Flexural properties of S‐2 glass and TR30S carbon fiber‐reinforced epoxy hybrid composites
Authors: Dong Chensong , Duong John , Davies Ian J. 
Published: April 2012

AbstractA study on the flexural properties of hybrid composites reinforced by S‐2 glass and TR30S carbon fibers is presented in this article. Test specimens were made by the hand lay‐up process in an intraply configuration with varying numbers of glass/epoxy laminas substituted for carbon/epoxy laminas. These specimens were then tested in the three point bend configuration in accordance with ASTM D790‐07 at a span to depth ratio of 32. The failed specimens were examined under an optical microscope, and the results show that the dominant failure mode is at the compressive side. The flexural behavior was also simulated by finite element analysis (FEA). Based on the FEA results, the flexural modulus and flexural strength were calculated. Good agreement is found between the experiments and FEA. It is shown that flexural modulus decreases with increasing percentage of S‐2 glass fibers, positive hybrid effects exist by substituting carbon fibers for glass fibers, and applying a thin layer of S‐2 glass fiber‐reinforced polymer on the compressive surface yields the highest flexural strength. The modeling approach presented will pave a way to the effective design of hybrid composites. POLYM. COMPOS., © 2012 Society of Plastics Engineers
Hyperbranched polymers as delivery vectors for oligonucleotides
Authors: Tan Jian Hong , McMillan Nigel A. J. , Payne Elizabeth , Alexander Cameron , Heath Felicity , Whittaker Andrew K. , Thurecht Kristofer J. 
Published: April 2012

AbstractWe report on the synthesis and characterization of hyperbranched dimethylaminoethyl methacrylate (DMAEMA) polymers using reversible addition fragmentation chain transfer polymerization. These polymers are unimolecular and globular and hence interact differently with DNA than conventional DMAEMA or block copolymers. The polymers were shown to effectively bind and condense oligonucleotides (ODNs); visualization of the bound complexes was achieved using atomic force microscopy, whereas isothermal titration calorimetry described the thermodynamics of binding. The ODNs were effectively protected from enzymatic degradation (DNAses) when condensed by all the polycations studied. However, internalization of the complexes into HeLa cells was less effective when the polycation was chain extended with polyethyleneglycol monomethylether methacrylate. Conjugation of folic acid to the periphery of the polycation facilitated much enhanced uptake of the oligomeric DNA into the HeLa cells due to overexpression of folate receptors on the surface of HeLa cells. Although significant cytotoxicity was observed at high polymer concentrations, this could be alleviated by shielding of the polycation using poly(ethyleneglycol monomethylether methacrylate). These results suggest that hyperbranched polymers formed in this way exhibit interesting complexation behavior with ODNs and thus are promising models to study as gene delivery vectors. © 2012 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2012