bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular imaging of p53 signal pathway in lung cancer cell cycle arrest induced by cisplatin
Authors: Wang Shenxu , Li Weijie , Xue Zengfu , Lu Yuanyuan , Narsinh Kazim , Fan Weiwei , Li Xiujuan , Bu Qingting , Wang Fu , Liang Jimin , Wu Kaichun , Cao Feng 
Published: June 2012

AbstractCisplatin is a commonly employed chemotherapy drug for lung malignancy. However its efficacy is limited by acquired drug resistance and lacking of an in vivo real‐time monitoring approach. The aim of this study is to investigate the effect of cisplatin on lung adenocarcinoma cell line p53‐RE‐Fluc/A549 in vivo via non‐invasive reporter gene by molecular imaging. For this study, we employed p53‐RE‐Fluc/A549 cells that overexpressed a vector with three tandem repeats of p53 response element followed by the luciferase reporter gene. P53 activity was evaluated by optical imaging and verified by Western blot after cells were exposed to 10 µM cisplatin for 72 h. The cell cycle was mainly blocked at the S‐ and G2/M‐phases after cisplatin treatment, whereas no significant change was observed in cell apoptotic index. Increased expression of p21 and Bcl‐2 as well as decreased expression of Bax were observed after cisplatin treatment by Western blotting. Longitudinal in vivo bioluminescent imaging (BLI) revealed that the p53 activity was increased from 24 to 48 h after transient cisplatin treatment in p53‐RE‐Fluc/A549‐bearing nude mice. RNA sequencing further revealed that cell cycle and p53 signaling pathway genes, such as E2F1, CCNA2, CDK1, and CCNE2 were significantly downregulated after long‐term cisplatin treatment. Thus, our study showed that cisplatin exerts its cytotoxic effect through blockage of the cell cycle and may be partly regulated by the p53 signaling pathway. Furthermore, molecular imaging is a useful tool to investigate the mechanism and evaluate the effect of chemotherapy drugs both in vivo and in vitro. © 2012 Wiley Periodicals, Inc.
Development of a differential suction electrode for improved intravaginal recordings of pelvic floor muscle activity: Reliability and motion artifact assessment
Authors: Keshwani Nadia , McLean Linda 
Published: June 2012

AbstractAimThe purposes of this study were to compare: (i) the reliability of electromyography (EMG) activity recorded from the pelvic floor muscles (PFMs) using a new differential suction electrode (DSE) to the reliability of EMG data recorded using other common electrodes, and (ii) motion artifact contamination of EMG activity recorded from the PFMs using the DSE and the Femiscan™ electrode.MethodsWith the DSE and the Femiscan™ in situ, at two separate sessions, each of 20 participants performed three repetitions of a maximum voluntary contraction (MVC) of their PFMs, and 10 repetitions of a maximal effort cough. With Delsys® electrodes located over the biceps brachii, each participant performed three repetitions of a MVC. Between‐trial and between‐day reliability were assessed using several methods. Motion artifact was assessed by comparing the proportion of contaminated files recorded by each electrode during coughing.ResultThe DSE was found to have excellent between‐trial reliability, as were the Femiscan™ and Delsys® electrodes. Between‐day reliability was good for the DSE, but reliability was higher for the Delsys® electrode and the Femiscan™ electrode. The DSE performed better than the Femiscan™ electrode in terms of motion artifact contamination.ConclusionsThe DSE has excellent between‐trial reliability and performs better than the Femiscan™ electrode in terms of motion artifact contamination. It does not perform as well as the Femiscan™ electrode in terms of between‐day reliability—a result that is not unexpected given the localized region from which the DSE records activity. Neurourol. Urodynam. 31:1272–1278, 2012. © 2012 Wiley Periodicals, Inc.
LUTS patient reported outcomes tool: Linguistic validation in 10 European languages
Authors: Brandt Barbara A. , Angün Çolpan , Coyne Karin S. , Doshi Sameepa , Bavendam Tamara , Kopp Zoe S. 
Published: June 2012

AbstractAimTo linguistically validate the translation of the Lower Urinary Tract Symptoms (LUTS) Tool fromEnglish to 10 European languages and assess whether the translations adequately reflected the concepts in the original version and if urological terms and the individual items were readily understood.MethodsResidents of the target countries who spoke the native language and had LUTS were asked to evaluate a harmonized translation of the LUTS Tool, which was developed in 10 languages: Danish (Denmark), Dutch (Belgium, Netherlands), Finnish (Finland), French (Belgium, France, Luxembourg, and Switzerland), German (Germany, Switzerland), Greek (Greece), Icelandic (Iceland), Italian (Italy), Norwegian (Norway), and Spanish (Spain). After reviewing the language‐appropriate version, participants were asked to explain why any words were difficult to understand and to paraphrase each sentence during a standardized cognitive debriefing interview. Linguistic validation teams (original translators, back translator, project manager, interviewer, and survey research expert) conducted a qualitative analysis of the cognitive debriefing findings.ResultsThe terminology and concepts in the English‐language version of the LUTS Tool were adequately captured in all language translations. The overall item comprehension rate, across languages, was 98.5%. Terms related to storage, voiding, and post‐micturition LUTS were comprehensible. Minor difficulties with comprehension were identified in 9 of 10 translations, and those changes were made to improve clarity.ConclusionsIndividuals with LUTS from 10 countries understood the individual items captured in translated versions of the LUTS Tool. The overall comprehension rate was high. Most changes were made to improve conceptual clarity based on country‐specific linguistic and cultural nuances. Neurourol. Urodynam. 32: 75–81, 2013. © 2012 Wiley Periodicals, Inc.
Sustained therapeutic effects of percutaneous tibial nerve stimulation: 24‐month results of the STEP study
Authors: Peters Kenneth M. , Carrico Donna J. , MacDiarmid Scott A. , Wooldridge Leslie S. , Khan Ansar U. , McCoy Craig E. , Franco Nicholas , Bennett Jason B. 
Published: June 2012

AbstractAimsTo evaluate the safety, sustained effectiveness, and treatment interval for percutaneous tibial nerve stimulation (PTNS) for overactive bladder (OAB) therapy through 24 months.MethodsA prospective study following treatment success after 12 weekly PTNS treatments, subjects were prescribed a 14‐week tapering protocol, followed by ongoing therapy with a Personal Treatment Plan determined by the investigator and subject to sustain subject OAB symptom improvement. Questionnaires were completed every 3 months, voiding diaries every 6 months; adverse events were reported throughout.ResultsOf 50 subjects enrolled, 35 remained in the study at 24 months. During the 24 months following initial treatment success and a 14‐week tapering protocol, mean treatments per month was 1.3. Voiding diary and OAB‐q data demonstrate sustained improvement reported at 13 weeks through 24 months. Improvements in frequency, urge incontinence episodes, night‐time voids and moderate‐to‐severe urgency episodes from voiding diaries at 6, 12, 18, and 24 months were statistically significant compared to baseline (prior to initial 12 weekly treatments). Compared to baseline, OAB‐q symptom severity scores and health related quality of life scores were statistically significant for improvement at each tested time point. Five mild adverse events of unknown relation to treatment were reported.ConclusionSustained safety and efficacy of PTNS were demonstrated over 24 months with initial success after 12 weekly treatments, followed by a 14‐week prescribed tapering protocol and a Personalized Treatment Plan. With an average of 1.3 treatments per month, PTNS therapy is a safe, durable, and valuable long‐term OAB treatment option to sustain clinically significant OAB symptom control. Neurourol. Urodynam. 32: 24–29, 2013. © 2012 Wiley Periodicals, Inc.
Motor cortex stimulation in refractory pelvic and perineal pain: Report of two successful cases
Authors: Louppe Jean‐Marie , Nguyen Jean‐Paul , Robert Roger , Buffenoir Kevin , de Chauvigny Edwige , Riant Thibault , Péréon Yann , Labat Jean‐Jacques , Nizard Julien 
Published: June 2012

AbstractAimsIn some patients, with refractory chronic pelvic and perineal pain, pain and quality of life are barely alleviated despite optimal medical treatment, infiltrations and surgical release of the pudendal nerve. The management of these patients is complex, especially after failure of neuromodulation techniques (spinal cord stimulation. S3 nerve root stimulation and direct stimulation of the pudendal nerve). We report the first two cases illustrating the value of motor cortex stimulation (MCS), in this new indication.MethodsThe history, decision‐making process, intraoperative findings and results of this technique are presented. The perineal cortical area was identified by intraoperative motor evoked potentials in the external anal sphincter, confirming its location in the primary motor cortex between the inferior and superior limb positions. As predictive value of repetitive transcranial magnetic stimulation (rTMS) in the identification of responders to MCS for pain is now established, we performed pre‐operative rTMS sessions for both patients.ResultsThe first patient was a 74‐years‐old woman who reported an 11‐year history of left lateral perineal pain. The second patient was a 45‐year‐old woman who reported a 4‐year history of perineal pain following hysterectomy with ovariectomy. After respectively 40 months and 19 months of follow up, both patients reported an improvement of pain ranging from 40 to 50%. Time to onset of pain on sitting was markedly improved from a few minutes to 90 minutes, and largely contributing to improvement of activities of daily living and of quality of life.ConclusionThese two first cases suggest that motor cortex stimulation constitutes a new treatment for refractory pelvic and perineal pain, and should be considered after failure of conventional neuromodulation techniques, especially spinal cord stimulation. Neurourol. Urodynam. 32: 53–57, 2013. © 2012 Wiley Periodicals, Inc.
“Meningitis‐retention syndrome”: A review
Authors: Sakakibara Ryuji , Kishi Masahiko , Tsuyusaki Yohei , Tateno Akihiko , Tateno Fuyuki , Uchiyama Tomoyuki , Yamamoto Tatsuya , Yamanishi Tomonori , Yano Masashi 
Published: June 2012

AbstractAimsA peculiar combination of acute urinary retention and aseptic meningitis has been described. This combination is referred to as meningitis‐retention syndrome (MRS), since patients with this syndrome exhibited no other abnormalities, except for mild pyramidal involvement. We aimed to delineate this syndrome by reviewing literatures.MethodsWe performed a systematic review of the literature to identify the frequency, clinical symptoms, urodynamic findings, putrative underlying pathology, and management of this syndrome.ResultsPatients with MRS have typical symptoms of fever, headache, stiff neck, and minor pyramidal signs, together with acute urinary retention. The bladder is initially areflexic, but soon becomes either normal or overactive in the repeated urodynamics during the course of the disorder. MRS is thought to be a very mild form of acute disseminated encephalomyelopathy (ADEM), with increased cell count, total protein, and occasional myelin basic protein in the cerebrospinal fluid. Proper management of the acute urinary retention is necessary to avoid bladder injury due to overdistension. The effectiveness of immune treatments (e.g., steroid pulse therapy) in shortening the urinary retention period awaits further study.ConclusionsAlthough rare, MRS is a disorder that both urologists and neurologists may encounter. MRS should be listed in the differential diagnosis of acute urinary retention. Neurourol. Urodynam. 32: 19–23, 2013. © 2012 Wiley Periodicals, Inc.
Model reduction for dynamical systems with quadratic output
Authors: Van Beeumen R. , Van Nimmen K. , Lombaert G. , Meerbergen K. 
Published: June 2012

SUMMARYFinite element models for structures and vibrations often lead to second order dynamical systems with large sparse matrices. For large‐scale finite element models, the computation of the frequency response function and the structural response to dynamic loads may present a considerable computational cost. Padé via Krylov methods are widely used and are appreciated projection‐based model reduction techniques for linear dynamical systems with linear output. This paper extends the framework of the Krylov methods to systems with a quadratic output arising in linear quadratic optimal control or random vibration problems. Three different two‐sided model reduction approaches are formulated based on the Krylov methods. For all methods, the control (or right) Krylov space is the same. The difference between the approaches lies, thus, in the choice of the observation (or left) Krylov space. The algorithms and theory are developed for the particularly important case of structural damping. We also give numerical examples for large‐scale systems corresponding to the forced vibration of a simply supported plate and of an existing footbridge. In this case, a block form of the Padé via Krylov method is used. Copyright © 2012 John Wiley & Sons, Ltd.
An element‐free Galerkin method for 3D crack propagation simulation under complicated stress conditions
Authors: Hu Yunjin , Yang Zhenjun 
Published: June 2012

SUMMARYThis study develops an element‐free Galerkin method based on the moving least‐squares approximation to trace three‐dimensional crack propagation under complicated stress conditions. The crack surfaces are modelled by a collection of planar triangles that are added when cracks propagate. The visibility criterion is adopted to treat the screening effect of the cracks on the influenced domain of a Gaussian point. Cracks are assumed to propagate in the perpendicular planes at crack front points when the strain energy release rates reach the material fracture toughness. This method is unique in that it uses a nonlinear contact iterative algorithm to consider contributions of crack surface interaction to the global equilibrium equations, so that crack opening, sliding and closing under complicated stress states can be efficiently modelled. Two numerical examples of three‐dimensional quasi‐static crack propagation were modelled with satisfactory results. Copyright © 2012 John Wiley & Sons, Ltd.
High resolution digital image correlation using proper generalized decomposition: PGD‐DIC
Authors: Passieux J.‐C. , Périé J.‐N. 
Published: June 2012

SUMMARYA new method is proposed to measure the finite element (FE) displacement field from a deformed image in comparison with a reference one. In opposition to standard FE approaches, the unknown displacement is sought as a sum of products of separated dimension functions. With the problems in each dimension being uncoupled, the method involves only one‐dimensional meshes and one‐dimensional problems. An algorithm that builds successive best rank‐one approximations is proposed and integrated into the nonlinear iterations of the correlation problem. Although the method can be applied to spaces of any dimension, this paper focuses on two‐dimensional images. Many synthetic examples are provided to evaluate the performance of the method. In addition, it is shown that, even with this separated representation, the introduction of a regularization operator is convenient. The latter makes it possible to perform a pixel‐wise measure with huge computational savings. Copyright © 2012 John Wiley & Sons, Ltd.
Second‐order accurate derivatives and integration schemes for meshfree methods
Authors: Duan Qinglin , Li Xikui , Zhang Hongwu , Belytschko Ted 
Published: June 2012

SUMMARYThe consistency condition for the nodal derivatives in traditional meshfree Galerkin methods is only the differentiation of the approximation consistency (DAC). One missing part is the consistency between a nodal shape function and its derivatives in terms of the divergence theorem in numerical forms. In this paper, a consistency framework for the meshfree nodal derivatives including the DAC and the discrete divergence consistency (DDC) is proposed. The summation of the linear DDC over the whole computational domain leads to the so‐called integration constraint in the literature. A three‐point integration scheme using background triangle elements is developed, in which the corrected derivatives are computed by the satisfaction of the quadratic DDC. We prove that such smoothed derivatives also meet the quadratic DAC, and therefore, the proposed scheme possesses the quadratic consistency that leads to its name QC3. Numerical results show that QC3 is the only method that can pass both the linear and the quadratic patch tests and achieves the best performances for all the four examples in terms of stability, convergence, accuracy, and efficiency among all the tested methods. Particularly, it shows a huge improvement for the existing linearly consistent one‐point integration method in some examples. Copyright © 2012 John Wiley & Sons, Ltd.
Who Benefits from KIPP?
Authors: Angrist Joshua D. , Dynarski Susan M. , Kane Thomas J. , Pathak Parag A. , Walters Christopher R. 
Published: June 2012

AbstractThe nation's largest charter management organization is the Knowledge is Power Program (KIPP). KIPP schools are emblematic of the No Excuses approach to public education, a highly standardized and widely replicated charter model that features a long school day, an extended school year, selective teacher hiring, strict behavior norms, and emphasizes traditional reading and math skills. No Excuses charter schools are sometimes said to target relatively motivated high achievers at the expense of students who are more difficult to teach, including limited English proficiency (LEP) and special education (SPED) students, as well as students with low baseline achievement levels. We use applicant lotteries to evaluate the impact of KIPP Academy Lynn, a KIPP school in Lynn, Massachusetts that typifies the KIPP approach. Our analysis focuses on special needs students that may be underserved. The results show average achievement gains of 0.36 standard deviations in math and 0.12 standard deviations in reading for each year spent at KIPP Lynn, with the largest gains coming from the LEP, SPED, and low‐achievement groups. Average reading gains are driven almost entirely by SPED and LEP students, whose reading scores rise by roughly 0.35 standard deviations for each year spent at KIPP Lynn.
Goserelin and bicalutamide treatments alter the expression of microRNAs in the prostate
Authors: Lehmusvaara Saara , Erkkilä Timo , Urbanucci Alfonso , Jalava Sanni , Seppälä Janne , Kaipia Antti , Kujala Paula , Lähdesmäki Harri , Tammela Teuvo L.J. , Visakorpi Tapio 
Published: June 2012

AbstractBACKGROUNDAlthough endocrine therapy has been used for decades, its influence on the expression of microRNAs (miRNAs) in clinical tissue specimens has not been analyzed. Moreover, the effects of the TMPRSS2:ERG fusion on the expression of miRNAs in hormone naïve and endocrine‐treated prostate cancers are poorly understood.METHODSWe used clinical material from a neoadjuvant trial consisting of 28 men treated with goserelin (n = 8), bicalutamide (n = 9), or no treatment (n = 11) for 3 months prior to radical prostatectomy. Freshly frozen specimens were used for microarray analysis of 723 human miRNAs. Specific miRNA expression in cancer, benign epithelium and stromal tissue compartments was predicted with an in silico Bayesian modeling tool.RESULTSThe expression of 52, 44, and 34 miRNAs was affected >1.4‐fold by the endocrine treatment in the cancer, non‐malignant epithelium, and stromal compartments, respectively. Of the 52 miRNAs, only 10 were equally affected by the two treatment modalities in the cancer compartment. Twenty‐six of the 52 genes (50%) showed AR binding sites in their proximity in either VCaP or LNCaP cell lines. Forty‐seven miRNAs were differentially expressed in TMPRSS2:ERG fusion positive compared with fusion negative cases. Endocrine treatment reduced the differences between fusion positive and negative cases.CONCLUSIONSGoserelin treatment and bicalutamide treatment mostly affected the expression of different miRNAs. The effect clearly varied in different tissue compartments. TMPRSS2:ERG fusion positive and negative cases showed differential expression of miRNAs, and the difference was diminished by androgen ablation. Prostate 73: 101–112, 2013. © 2012 Wiley Periodicals, Inc.
Ardisianone, a natural benzoquinone, efficiently induces apoptosis in human hormone‐refractory prostate cancers through mitochondrial damage stress and survivin downregulation
Authors: Yu Chia‐Chun , Wu Ping‐Jung , Hsu Jui‐Ling , Ho Yunn‐Fang , Hsu Lih‐Ching , Chang Yu‐Jia , Chang Hsun‐Shuo , Chen Ih‐Sheng , Guh Jih‐Hwa 
Published: June 2012

AbstractBACKGROUNDIncreasing evidence suggests that mitochondria play a central role in regulating cell apoptosis. Survivin, an inhibitor of apoptosis protein (IAP) family member, mediates resistance to cancer chemotherapy particularly in prostate cancers. Therefore, development of anticancer agents targeting mitochondria and survivin is a potential strategy.METHODCell proliferation was examined by sulforhodamine B, CFSE staining, and clonogenic assays. Mitochondrial membrane potential (ΔΨm) and reactive oxygen species (ROS) were detected by flow cytometric analysis. Protein expression was detected by Western blot. RNA levels were examined by reverse transcription polymerase chain reaction assay. Overexpression of constitutively active Akt was also used in this study.RESULTSArdisianone, a natural benzoquinone derivative, displayed anti‐proliferative and apoptotic activities against human hormone‐refractory prostate cancer cells (HRPC), PC‐3, and DU‐145. Ardisianone dramatically induced mitochondrial damage, identified by downregulation of Bcl‐2 family proteins, ROS production, and loss of ΔΨm. Ardisianone also inhibited Akt and mTOR/p70S6K pathways and induced a fast downregulation of survivin, leading to activation of mitochondria‐involved caspase cascades. Overexpression of constitutively active Akt partly rescued ardisianone‐mediated apoptotic signaling cascades. Furthermore, a long‐term treatment of ardisianone caused an increase of endoplasmic reticulum (ER) stress, upregulation of cIAP1 and cIAP2, and apoptosis‐inducing factor (AIF)‐mediated caspase‐independent apoptosis.CONCLUSIONSThe data suggest that the ardisianone induces apoptosis in human prostate cancers through mitochondrial damage stress, leading to the inhibition of mTOR/p70S6K pathway, downregulation of Bcl‐2 family members, degradation of survivin, and activation of caspase cascades. The data provide evidence supporting that ardisianone is a potential anticancer agent against HRPCs. Prostate 73: 133–145, 2013. © 2012 Wiley Periodicals, Inc.