bluuebunny/crossref_metadata_2025_split huggingface dataset:
The subcellular location of antigen expressed by adenoviral vectors modifies adaptive immunity but not dependency on cross‐presenting dendritic cells
Authors: Henning Petra , Gustafsson Tobias , Flach Carl‐Fredrik , Hua Yeu‐Jiann , Strömbeck Anna , Holmgren Jan , Lindholm Leif , Yrlid Ulf 
Published: May 2011

AbstractAdenoviral (Ad) vaccine vectors can generate protective immunity to various pathogens in animal studies. However, recent failures in clinical vaccine trials have underscored the need for a better understanding of how mucosal immune responses to Ad‐encoded vaccine Ags are generated in vivo. In this study, we addressed whether directing Ad‐encoded ovalbumin (OVA) to different subcellular compartments influences the generation of OVA‐specific acquired immunity and the APCs required following i.n. immunization of mice. We show that both secreted and membrane‐anchored OVA activate CD4+ T cells, induce cytotoxic CD8+ T lymphocytes (CTLs) and generate serum IgG. Additionally, vaginal IgG is induced when OVA is expressed at these subcellular locations, but only the secreted form generates a significant IgA response in the lungs. On the contrary, intracellular expression of OVA efficiently expands CD8+ T cells but fails to activate CD4+ T cells, results in poor CTL activity, and does not generate Abs. Finally, we show that regardless of the subcellular localization of OVA, conventional DCs (cDCs) are required for the activation of T cells. However, the direct transduction of conventional DCs is not essential. These findings have important implications for the improvement of Ad vector design and vaccine‐induced mucosal immunity.
Structural insights into the binding of hepatitis B virus core peptide to HLA‐A2 alleles: Towards designing better vaccines
Authors: Liu Jingxian , Chen Kenneth Y. , Ren Ee C. 
Published: May 2011

AbstractBinding of specific antigenic peptides with human leukocyte antigen (HLA) molecules is a prerequisite for the initiation of T‐cell responses and structural information about the peptide–HLA complex is essential for the detailed understanding of such interactions. HLA‐A2 is the most prevalent HLA allele globally but aside from A*02:01 there is a significant lack of crystal structures, particularly for alleles that occur in high frequencies among Asian populations. Here, we report three HLA‐A2 structures with the immunodominant hepatitis B core antigen 18–27 (HBcAg18–27) epitope, namely A*02:03, A*02:06, and A*02:07 at resolutions of 2.16, 1.70, and 1.75 Å respectively. This comparative analysis reveals that minor polymorphic residue changes between different HLA alleles can induce significant alterations in the major histocompatibility complex–peptide interface, and introduce conformational changes in the p3–p8 peptide region. Circular dichroism analysis demonstrated the HLA‐A2–peptide complexes to have a hierarchy of thermostability and binding affinity in the order of A*02:06>A*02:07>A*02:01>A*02:03. Our findings provide structural insights into the varied HLA‐A2 allele binding of the hepatitis B core antigen 18–27 epitope and the data suggest that chemical modifications of the peptide side chains could be a promising strategy to modulate and improve HLA‐A2–peptide binding affinity for vaccine design.
Synthesis and Characterization of a Series of Ruthenium Tris(β‐diketonato) Complexes by an UHV‐STM Investigation and Numerical Calculations
Authors: Munery Sabrina , Ratel‐Ramond Nicolas , Benjalal Youness , Vernisse Loranne , Guillermet Olivier , Bouju Xavier , Coratger Roland , Bonvoisin Jacques 
Published: May 2011

AbstractA series of ruthenium tris(β‐diketonate) complexes was investigated by using electrochemistry, UV/Vis spectroscopy, 1H NMR and 13C NMR spectroscopy and FAB mass spectroscopy. Several new mononuclear mixed‐ligand ruthenium(III) complexes were prepared: with three dibenzoylmethanate ions (dbm) {[Ru(dbm)3] (1)}, one or two acetylacetonate ions (2,4‐pentanedionate, acac) {[Ru(dbm)2(acac)] (2), [Ru(dbm)(acac)2] (3)}, two acetonitrile ligands {[Ru(dbm)2(CH3CN)2][CF3SO3] (4)} or a functionalized acetylacetonate ion {[Ru(dbm)2(acac‐I)] (5) (acac‐I = 3‐iodo‐2,4‐pentanedionate ion), [Ru(dbm)2(acac‐Br)] (6) (acac‐Br = 3‐bromo‐2,4‐pentanedionate ion)}. In addition, X‐ray structures for complexes 1, 2, 3, 4 and 6 were determined. Scanning tunnelling microscopy measurements at liquid He temperature and in anultra‐high vacuum (UHV‐STM) were conducted on complex 1 on a Ag(111) surface. This indicates that the complex can be successfully evaporated and observed after adsorption onto a metallic substrate. Analysis of the STM images, supported by adsorption and STM image calculations, demonstrates that the molecules exist in two stable forms when adsorbed onto the surface.
The role of self‐esteem contingencies in the distinction between obsessive and harmonious passion
Authors: Mageau Geneviève A. , Carpentier Joëlle , Vallerand Robert J. 
Published: May 2011

AbstractThe Dualistic Model of Passion (Vallerand et al., 2003) shows that people can experience a harmonious or an obsessive passion toward an activity. Mageau and Vallerand (2007; Mageau et al., 2009) have argued that self‐related processes, such as contingencies of self‐worth, are central in the distinction between the two types of passion. Specifically, it was proposed that people with an obsessive passion rely more heavily on their passionate activity to derive self‐esteem than people with a harmonious passion such that they should experience self‐esteem fluctuations as a function of their performances in their passionate activity. This study tested this hypothesis. Using self‐reports, results first showed that the more people have an obsessive passion the more they report experiencing self‐esteem fluctuations that covary with their performances in their passionate activity. In contrast, people with a harmonious passion did not report experiencing more, or less, self‐esteem fluctuations. Second, hierarchical linear modeling confirmed that, in a real‐life setting, the more people report an obsessive passion toward a card game, the greater is the impact of performance on their state self‐esteem. Taken together, these findings suggest that obsessive, but not harmonious, passion triggers contingencies between people's self‐esteem and their passionate activity. Copyright © 2011 John Wiley & Sons, Ltd.
Cyclic Voltammetry Study of the Mn‐Substituted Polyoxoanions [MnII4(H2O)2(H4AsW15O56)2]18− and [((MnIIOH2)MnII2PW9O34)2(PW6O26)]17−: Electrodeposition of Manganese Oxides Electrocatalysts for Dioxygen Reduction
Authors: Ammam Malika , Keita Bineta , Nadjo Louis , Mbomekalle Israel‐Martyr , Ritorto Michelle D. , Anderson Travis M. , Neiwert Wade A. , Hill Craig L. , Fransaer Jan 
Published: May 2011

AbstractThe electrochemical behavior of two manganese (Mn)‐substituted polyoxoanions, the dissymmetrical Dawson sandwich‐type [MnII4(H2O)2(H4AsW15O56)2]18− and the Keggin sandwich banana‐shaped [((MnIIOH2)MnII2PW9O34)2(PW6O26)]17− is investigated. At pH 5, the oxidation of the MnII‐centers results in one oxidation wave for [MnII4(H2O)2(H4AsW15O56)2]18− and two oxidation waves for [((MnIIOH2)MnII2PW9O34)2(PW6O26)]17−. To the best of our knowledge, presence of the second Mn‐based wave is rarely observed in the electrochemistry of Mn‐containing polyoxometalates. Deposition of Mn‐oxides electrocatalysts for dioxygen reduction is noticed by cyclic voltammetry, which can be distinguished by the significant positive shift in potentials of the dioxygen reduction reaction.
Genotyping of single nucleotide polymorphism in γ‐glutamyl hydrolase gene by capillary electrophoresis
Authors: Cheng Hui‐Ling , Chiou Shyh‐Shin , Liao Yu‐Mei , Chen Yen‐Ling , Wu Shou‐Mei 
Published: May 2011

AbstractThe γ‐glutamyl hydrolase (GGH) gene plays an important role in methotrexate (MTX) metabolism, ensuring that MTX polyglutamates (MTX‐(Glu)n) could be converted back into MTX. Accumulation of MTX‐(Glu)n is a problem in MTX therapy. SNP 452 C>T has been reported to associate with lower catalytic activity and higher accumulation of long‐chain MTX‐(Glu)n in patients treated with higher doses of MTX treatment. We propose and establish a simple and effective CE method for detecting SNP in GGH gene. The DNA samples after amplification were analyzed by SSCP‐CE method. The CE conditions were generated by using 1× TBE buffer containing 1.5% w/v hydroxypropyl methyl cellulose under reverse polarity at 25°C. This method was applied to detect genotyping of acute lymphoblastic leukemia patients receiving MTX treatment. The results were confirmed by DNA sequencing with good agreement. Concentrations of MTX‐(Glu)n in whole blood were analyzed by on‐line stacking CE method. MTX‐(Glu)n levels and genotypes in GGH gene of acute lymphoblastic leukemia patients were evaluated. The SSCP‐CE method was found to be feasible for SNP screening in the GGH gene.
Analysis of mutations in the Pig‐a gene of spleen T‐cells from N‐ethyl‐N‐nitrosourea‐treated fisher 344 rats
Authors: Miura Daishiro , Shaddock Joseph G. , Mittelstaedt Roberta A. , Dobrovolsky Vasily N. , Kimoto Takafumi , Kasahara Yoshinori , Heflich Robert H. 
Published: May 2011

AbstractA rapid in vivo somatic cell gene mutation assay is being developed that measures mutation in the endogenous X‐linked phosphatidylinositol glycan, class A gene (Pig‐a). The assay detects Pig‐a mutants by flow cytometric identification of cells deficient in glycosylphosphatidyl inositol (GPI) anchor synthesis. GPI‐deficient, presumed Pig‐a mutant cells also can be detected in a cloning assay that uses proaerolysin (ProAER) selection. Previously, we demonstrated that ProAER‐resistant (ProAERr) rat spleen T‐cells have mutations in the Pig‐a gene. In the present study, we report on a more complete analysis of ProAERr rat spleen T‐cell mutants and describe a mutation spectrum for mutants isolated from rats 4 weeks after treatment with three consecutive doses of 35.6 mg/kg N‐ethyl‐N‐nitrosourea (ENU). We identified a total of 55 independent mutations, with the largest percentage (69%) involving basepair substitution at A:T. The overall spectrum of Pig‐a gene mutations was consistent with the types of DNA adducts formed by ENU and was very similar to what has been described for in vivo ENU‐induced mutation spectra in other rodent reporter genes (e.g., in the endogenous Hprt gene and transgenic shuttle vectors). These data are consistent with the rat Pig‐a assay detecting test‐agent‐induced mutational responses. Environ. Mol. Mutagen., 2011. Published 2011 Wiley‐Liss, Inc.
Life‐cycle reliability of RC bridge piers under seismic and airborne chloride hazards
Authors: Akiyama Mitsuyoshi , Frangopol Dan M. , Matsuzaki Hiroshi 
Published: May 2011

AbstractOver the last two decades, the probabilistic assessment of reinforced concrete (RC) structures under seismic hazard has been developed rapidly. However, little attention has been devoted to the assessment of the seismic reliability of corroded structures. For the life‐cycle assessment of RC structures in a marine environment and earthquake‐prone regions, the effect of corrosion due to airborne chlorides on the seismic capacity needs to be taken into consideration. Also, the effect of the type of corrosive environment on the seismic capacity of RC structures has to be quantified.In this paper, the evaluation of the displacement ductility capacity based on the buckling model of longitudinal rebars in corroded RC bridge piers is established, and a novel computational procedure to integrate the probabilistic hazard associated with airborne chlorides into life‐cycle seismic reliability assessment of these piers is proposed. The seismic demand depends on the results of seismic hazard assessment, whereas the deterioration of seismic capacity depends on the hazard associated with airborne chlorides. In an illustrative example, an RC bridge pier was modeled as single degree of freedom (SDOF). The longitudinal rebars buckling of this pier was considered as the sole limit state when estimating its failure probability. The findings show that the life‐cycle reliability of RC bridge piers depends on both the seismic and airborne chloride hazards, and that the cumulative‐time failure probabilities of RC bridge piers located in seismic zones can be dramatically affected by the effect of airborne chlorides. Copyright © 2011 John Wiley & Sons, Ltd.
Synthesis and optical properties of a bis(diphenylphosphino)dithienosilole‐digold(I) complex
Authors: Ohshita Joji , Tominaga Yuta , Mizumo Tomonobu , Kuramochi Yusuke , Higashimura Hideyuki 
Published: May 2011

AbstractTreatment of 2,6‐bis(diphenylphosphino)‐4,4‐bis(4‐n‐butylphenyl)dithienosilole with chloro(dimethylsulfide)gold(I) in acetone gave the corresponding digold complex as pale green solids. The complex was highly emissive and showed blue photoluminescence with the quantum efficiencies of Φ = 0.99 and 0.30 for its solution and powders, respectively. The complex showed good film forming properties and its spin coating from the toluene solution afforded a thin solid film that also showed emissive properties with Φ = 0.08 © 2011 Wiley Periodicals, Inc. Heteroatom Chem 22:514–517, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/hc.20715
Free radical addition of α‐telluroesters to alkenes
Authors: Kambe Nobuaki , Han Li‐Biao , Fujiwara Shin‐ichi , Sonoda Noboru 
Published: May 2011

Abstractα‐(Phenyltelluro)esters 2 reacted with electron‐rich alkenes 3 in the presence of a catalytic amount of azobisisobutyronitrile (AIBN) as an initiator to give the corresponding addition products 4. This carbotelluration of alkenes proceeded via a radical chain mechanism involving the bimolecular homolytic substitution (SH2) reaction on tellurium as the key step, wherein tellurium plays an important role in a kinetic fashion to realize the rapid group transfer process resulting in suppressing the competing olefin polymerization. © 2011 Wiley Periodicals, Inc. Heteroatom Chem 22:518–522, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/hc.20716
Conjugate addition of vinylic organocuprates generated via transmetalation of phenylseleno‐substituted vinylzirconates: Functionalization at the 4‐position of enones
Authors: Segi Masahito , Suzuki Masahiro , Shintaku Kazuki , Maeda Hajime 
Published: May 2011

AbstractHydrozirconation of propargylic selenides (1) or 4‐phenylseleno‐1‐butyne (2) with Cp2ZrHCl, followed by in situ transmetalation to cuprate (Me2Cu(CN)Li2) and addition of enones, led to the formation of 1,4‐adducts in good yields. The adducts derived from 1 were subjected to oxidation with H2O2 to afford allylic alcohol derivatives via [2,3] sigmatropic rearrangement of allylic selenides. On the other hand, the oxidation of the products derived from 2 gave the corresponding ketones having a conjugated diene moiety at the β‐position via selenoxide elimination. © 2011 Wiley Periodicals, Inc. Heteroatom Chem 22:545–552, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/hc.20720
Choline supplementation mitigates trace, but not delay, eyeblink conditioning deficits in rats exposed to alcohol during development
Authors: Thomas Jennifer D. , Tran Tuan D. 
Published: May 2011

AbstractChildren exposed to alcohol prenatally suffer from a range of physical, neuropathological, and behavioral alterations, referred to as fetal alcohol spectrum disorders (FASD). Both the cerebellum and hippocampus are affected by alcohol exposure during development, which may contribute to behavioral and cognitive deficits observed in children with FASD. Despite the known neuropathology associated with prenatal alcohol exposure, many pregnant women continue to drink (heavy drinkers, in particular), creating a need to identify effective treatments for their children who are adversely affected by alcohol. We previously reported that choline supplementation can mitigate alcohol's effects on cognitive development, specifically on tasks which depend on the functional integrity of the hippocampus. The present study examined whether choline supplementation could differentially mitigate alcohol's effects on trace eyeblink classical conditioning (ECC, a hippocampal‐dependent task) and delay ECC (a cerebellar‐dependent task). Long‐Evans rats were exposed to 5.25 g/kg/day alcohol via gastric intubation from postnatal days (PD) 4–9, a period of brain development equivalent to late gestation in humans. A sham‐intubated control group was included. From PD 10–30, subjects received subcutaneous injections of 100 mg/kg choline chloride or vehicle. Beginning on PD 32–34, subjects were trained on either delay or trace eyeblink conditioning. Performance of subjects exposed to alcohol was significantly impaired on both tasks, as indicated by significant reductions in percentage and amplitude of conditioned eyeblink responses, an effect that was attenuated by choline supplementation on the trace, but not delay conditioning task. Indeed, alcohol‐exposed subjects treated with choline performed at control levels on the trace eyeblink conditioning task. There were no significant main or interactive effects of sex. These data indicate that choline supplementation can significantly reduce the severity of trace eyeblink conditioning deficits associated with early alcohol exposure, even when administered after the alcohol insult is complete. These findings have important implications for the treatment of fetal alcohol spectrum disorders. © 2011 Wiley Periodicals, Inc.
Post‐training cocaine exposure facilitates spatial memory consolidation in C57BL/6 mice
Authors: Iñiguez Sergio D. , Charntikov Sergios , Baella Shelley A. , Herbert Matthew S. , Bolaños‐Guzmán Carlos A. , Crawford Cynthia A. 
Published: May 2011

AbstractIn this study, we examined the ability of post‐training injections of cocaine to facilitate spatial memory performance using the Morris water maze (MWM). We also investigated the role that hippocampal protein kinase A (PKA) and extracellular signal‐regulated kinase 1/2 (ERK) signaling may play in cocaine‐mediated spatial memory consolidation processes. Male and female C57BL/6 mice were first trained in a MWM task (eight consecutive trials) then injected with cocaine (0, 1.25, 2.5, 5, or 20 mg/kg), and memory for the platform location was retested after a 24 h delay. Cocaine had a dose‐dependent effect on spatial memory performance because only the mice receiving 2.5 mg/kg cocaine displayed a significant reduction in latency to locate the platform. No sex differences in MWM performance were observed; however, females showed higher hippocampal levels of PKA when compared with males. A second experiment demonstrated that 2.5 mg/kg cocaine enhanced MWM performance only when administered within 2, but not 4 h after spatial training. We also found that cocaine (2.5 mg/kg) increased ERK2 phosphorylation within the hippocampus and one of its downstream targets (ribosomal S6 kinase), a mechanism that may be responsible, at least in part, for the enhanced cocaine‐mediated spatial memory performance. Overall, these data demonstrate that a low dose of cocaine (2.5 mg/kg) administered within 2 h after training facilitates MWM spatial memory performance in C57BL/6 mice. © 2011 Wiley Periodicals, Inc.
Context‐dependent modulation of hippocampal and cortical recruitment during remote spatial memory retrieval
Authors: Lopez Joëlle , Herbeaux Karin , Cosquer Brigitte , Engeln Michel , Muller Christophe , Lazarus Christine , Kelche Christian , Bontempi Bruno , Cassel Jean‐Christophe , de Vasconcelos Anne Pereira 
Published: May 2011

AbstractAccording to systems consolidation, as hippocampal‐dependent memories mature over time, they become additionally (or exclusively) dependent on extra‐hippocampal structures. We assessed the recruitment of hippocampal and cortical structures on remote memory retrieval in a performance‐degradation resistant (PDR; no performance degradation with time) versus performance‐degradation prone (PDP; performance degraded with time) context. Using a water‐maze task in two contexts with a hidden platform and three control conditions (home cage, visible platform with or without access to distal cues), we compared neuronal activation (c‐Fos imaging) patterns in the dorsal hippocampus and the medial prefrontal cortex (mPFC) after the retrieval of recent (5 days) versus remote (25 days) spatial memory. In the PDR context, the hippocampus exhibited greater c‐Fos protein expression on remote than recent memory retrieval, be it in the visible or hidden platform group. In the PDP context, hippocampal activation increased at the remote time point and only in the hidden platform group. In the anterior cingulate cortex, c‐Fos expression was greater for remote than for recent memory retrieval and only in the PDR context. The necessity of the mPFC for remote memory retrieval in the PDR context was confirmed using region‐specific lidocaine inactivation, which had no impact on recent memory. Conversely, inactivation of the dorsal hippocampus impaired both recent and remote memory in the PDR context, and only recent memory in the PDP context, in which remote memory performance was degraded. While confirming that neuronal circuits supporting spatial memory consolidation are reorganized in a time‐dependent manner, our findings further indicate that mPFC and hippocampus recruitment (i) depends on the content and perhaps the strength of the memory and (ii) may be influenced by the environmental conditions (e.g., cue saliency, complexity) in which memories are initially formed and subsequently recalled. © 2011 Wiley Periodicals, Inc.
Activation of immobility‐related hippocampal theta by cholinergic septohippocampal neurons during vestibular stimulation
Authors: Tai Siew Kian , Ma Jingyi , Ossenkopp Klaus‐Peter , Leung L. Stan 
Published: May 2011

AbstractThe vestibular system has been suggested to participate in spatial navigation, a function ascribed to the hippocampus. Vestibular stimulation during spatial navigation activates a hippocampal theta rhythm (4–10 Hz), which may enhance spatial processing and motor response. We hypothesize that a cholinergic, atropine‐sensitive theta is generated during passive whole‐body rotation in freely behaving rats. Hippocampal EEGs were recorded by implanted electrodes in CA1 while rats were rotated on a vertical axis, for a minute or longer, at different angular velocities. Rotation induced a continuous hippocampal theta rhythm while the rat was immobile, in both light and dark conditions. Theta peak frequency showed a significant increase during high (50–70 rpm) as compared with a lower (20–49 rpm) rotational velocity. Rotation‐induced theta was abolished by muscarinic receptor antagonist atropine sulfate (50 mg/kg i.p.) but not by atropine methyl nitrate (50 mg/kg i.p.), which did not pass the blood‐brain barrier. Theta was attenuated in rats in which cholinergic neurons in the medial septum (MS) were lesioned with 192 IgG‐saporin (0.14 μg in 0.4 μl), as confirmed by depletion of MS cells immunoreactive to choline acetyltransferase and an absence of acetylcholinesterase staining in the hippocampus. Bilateral lesion of the vestibular receptors by sodium arsanilate (30 mg in 0.1 ml, intratympanically) also attenuated the rotation‐induced theta rhythm. In intact rats, field excitatory postsynaptic potentials (fEPSPs) in CA1 evoked by commissural stimulation were smaller during walking or rotation as compared with during immobility. Modulation of fEPSP was absent following atropine sulfate in intact rats and in 192 IgG‐saporin lesion rats. In summary, this is the first report of a continuous atropine‐sensitive hippocampal theta in the rat induced by vestibular stimulation during rotation, and accompanied by cholinergic modulation of hippocampal synaptic transmission. Vestibular‐activated septohippocampal cholinergic activity could be an important component in sensorimotor processing and spatial memory. © 2011 Wiley Periodicals, Inc.