bluuebunny/crossref_metadata_2025_split huggingface dataset:
TGF-β–Activated Kinase 1 Signaling Maintains Intestinal Integrity by Preventing Accumulation of Reactive Oxygen Species in the Intestinal Epithelium
Authors: Kajino-Sakamoto Rie , Omori Emily , Nighot Prashant K. , Blikslager Anthony T. , Matsumoto Kunihiro , Ninomiya-Tsuji Jun 
Published: September 2010

Abstract
The intestinal epithelium is constantly exposed to inducers of reactive oxygen species (ROS), such as commensal microorganisms. Levels of ROS are normally maintained at nontoxic levels, but dysregulation of ROS is involved in intestinal inflammatory diseases. In this article, we report that TGF-β–activated kinase 1 (TAK1) is a key regulator of ROS in the intestinal epithelium. tak1 gene deletion in the mouse intestinal epithelium caused tissue damage involving enterocyte apoptosis, disruption of tight junctions, and inflammation. Disruption of TNF signaling, which is a major intestinal damage inducer, rescued the inflammatory conditions but not apoptosis or disruption of tight junctions in the TAK1-deficient intestinal epithelium, suggesting that TNF is not a primary inducer of the damage noted in TAK1-deficient intestinal epithelium. We found that TAK1 deficiency resulted in reduced expression of several antioxidant-responsive genes and reduced the protein level of a key antioxidant transcription factor NF-E2–related factor 2, which resulted in accumulation of ROS. Exogenous antioxidant treatment reduced apoptosis and disruption of tight junctions in the TAK1-deficient intestinal epithelium. Thus, TAK1 signaling regulates ROS through transcription factor NF-E2–related factor 2, which is important for intestinal epithelial integrity.
Peptide Length Extension Skews the Minor HA-1 Antigen Presentation toward Activated Dendritic Cells but Reduces Its Presentation Efficiency
Authors: Hambach Lothar , Aghai Zohara , Pool Jos , Kröger Nicolaus , Goulmy Els 
Published: September 2010

Abstract
Minor histocompatibility Ags (mHags) are important targets of the graft-versus-leukemia effect after HLA-matched allogeneic stem cell transplantation. mHags are HLA-restricted polymorphic peptides expressed on normal and leukemia cells. Vaccination with hematopoiesis-restricted mHag peptides, such as HA-1, may boost the graft-versus-leukemia effect. However, some animal studies indicate that peptides exactly reflecting immunogenic T cell epitopes (short peptides [SPs]) induce tolerance that is potentially due to systemic Ag spreading. Peptide length extension (long peptides [LPs]) may optimize immune responses by restricting and prolonging Ag presentation on dendritic cells (DCs). In this study, we compared the in vitro characteristics and T cell-stimulatory capacities of a human 30-mer HA-1 LP with the 9-mer HA-1 SP. DCs presented the HA-1 LP and SP and expanded HA-1–specific cytotoxic T cell lines. As hypothesized, HA-1 LP presentation, but not SP presentation, was largely restricted to activated DCs and was nearly absent on other hematopoietic cells. However, DCs presented the HA-1 LP 2–3 log levels less efficiently than the SP. Finally, the decay of HA-1 LP and SP presentation on DCs was comparable. We conclude that HA-1 LP and SP differ in their in vitro characteristics and that only comparative clinical studies after allogeneic stem cell transplantation may reveal the optimal HA-1 vaccine.
Cellular Requirements for Diabetes Induction in DO11.10xRIPmOVA Mice
Authors: Wesley Johnna D. , Sather Blythe D. , Perdue Nikole R. , Ziegler Steven F. , Campbell Daniel J. 
Published: September 2010

Abstract
Type 1 diabetes (T1D) results from the immune-mediated destruction of the insulin-producing β-islet cells in the pancreas. The genetic and environmental mechanisms promoting the development of this disease remain poorly understood. We have explored the cellular requirements for T1D development in DO11.10xRIPmOVA (DORmO) mice, which carry a TCR transgene specific for an MHC class II-restricted epitope from OVA and express membrane-bound OVA in the pancreas under the control of the rat insulin promoter. We found that DORmO.RAG2−/− mice do not develop insulitis and are completely protected from diabetes, demonstrating that endogenous lymphocyte receptor rearrangement is required for disease development. Diabetes in DORmO mice is preceded by the development of OVA-specific autoantibodies and is delayed in B cell-deficient DORmO.JhD−/− mice, demonstrating that B cells contribute to disease progression. In addition, transfer of CD8+ T cells from diabetic animals into DORmO.RAG2−/− mice promoted insulitis by OVA-specific CD4+ T cells. Finally, although diabetes develops in DORmO mice in the presence of a significant population of Foxp3+ OVA-specific regulatory T cells, boosting regulatory T cell numbers by injecting IL-2 immune complexes dampens autoantibody production and prevents development of insulitis and overt diabetes. These results help define the events leading to diabetes in DORmO mice and provide new insights into the cellular interactions required for disease development in an Ag-specific model of T1D.
Effect of Ly49 Haplotype Variance on NK Cell Function and Education
Authors: Patel Rajen , Bélanger Simon , Tai Lee-Hwa , Troke Angela D. , Makrigiannis Andrew P. 
Published: September 2010

Abstract
The class I MHC-specific receptors expressed by murine NK cells exhibit remarkable variation. Specific activating killer Ig-related receptor/Ly49 have major effects on autoimmune and infectious disease induction and outcome in humans and mice. However, these studies are greatly affected by individual background genetics. Furthermore, the educational impact of variable inhibitory KIR/Ly49 gene numbers on NK cell development and the subsequent ability to survey for MHC class I (MHC-I) expression remain unknown. To address these questions, Ly49 congenic mice were generated that maintain a 129-derived Ly49 gene cluster on a C57BL/6 genetic background (B6.Ly49129 mice), and the in vitro and in vivo NK cell function of these mice was compared with their inbred parental 129S1 and C57BL/6 counterparts. Notably, target cell recognition directed by activating Ly49 receptors was profoundly affected by allelic variation in B6.Ly49129 congenic cells versus C57BL/6 NK cells. Furthermore, when assessing NK cell function based on education and subsequent recognition of the C57BL/6 MHC-I haplotype by inhibitory Ly49 receptors, B6.Ly49129 congenic mice exhibited robust NK cell activity, demonstrating efficient NK cell education by the 129S1 Ly49 cluster during development. The responsiveness of NK cells expressing 129S1 Ly49 was shown to be mediated by subsets expressing one or more self-MHC receptors, including Ly49I, Ly49O, Ly49V, and NKG2A. These findings demonstrate that the genetically segregating and diverse MHC-I and Ly49 loci in mice exhibit independent and epistatic effects on NK cell education that can be uncoupled during the intercrossing of inbred strains.
Glioma Cells Display Complex Cell Surface Topographies That Resist the Actions of Cytolytic Effector Lymphocytes
Authors: Hoa Neil , Ge Lisheng , Kuznetsov Yurii , McPherson Alex , Cornforth Andrew N. , Pham Jimmy T. H. , Myers Michael P. , Ahmed Nabil , Salsman Vita S. , Lamb Lawrence S. , Bowersock Joscelyn E. , Hu Yuanjie , Zhou Yi-Hong , Jadus Martin R. 
Published: September 2010

Abstract
Gliomas are invasive cancers that resist all forms of attempted therapy. Immunotherapy using Ag-pulsed dendritic cells has improved survival in some patients. We present evidence that another level of complexity may also contribute to lack of responses by the lymphocytes toward gliomas. Atomic force microscopy of four different glioma types—human U251 and rat T9 and F98 glioma cells, including freshly isolated human glioblastoma multiforme neurosphere cultures (containing “stem cell-like cells”)—revealed a complex surface topography with numerous microvilli and filopodia. These structures were not found on other cell types. Electron microscopy and immunofluorescence microscopy of glioma cells confirmed that microvilli are present. U251 cells with microvilli resisted the cytolytic actions of different human effector cells, (lymphokine-activated killer cells, γδ T cells, conventional CTLs, and chimeric Ag-receptor–redirected T cells) better than their nonmicrovilli-expressing counterparts. Killer lymphocytes released perforin, which was detected within the glioma’s microvilli/filopodia, indicating these structures can receive the cytolytic effector molecules, but cytotoxicity is suboptimal. Air-dried gliomas revealed nodes within the microvilli/filopodia. The microvilli that penetrated 0.4-μm transwell chamber’s pores resisted the actions of CTLs and physical damage. Those nodelike structures may represent a compartmentalization that resists physical damage. These microvilli may play multiple roles in glioma biology, such as invasion and resistance to lymphocyte-mediated killing.
NK Cell Deficiency Predisposes to Viral-Induced Th2-Type Allergic Inflammation via Epithelial-Derived IL-25
Authors: Kaiko Gerard E. , Phipps Simon , Angkasekwinai Pornpimon , Dong Chen , Foster Paul S. 
Published: September 2010

Abstract
Severe respiratory syncytial virus (RSV) infection has long been associated with an increased risk for the development of childhood asthma and exacerbations of this disorder. Despite much research into the induction of Th2 responses by allergens and helminths, the factors associated with viral infection that predispose to Th2-regulated asthma remain unknown. Recently, clinical studies have shown reduced numbers of NK cells in infants suffering from a severe RSV infection. Here we demonstrate that NK cell deficiency during primary RSV infection of BALB/c mice results in the suppression of IFN-γ production and the development of an RSV-specific Th2 response and subsequent allergic lung disease. The outgrowth of the Th2 responses was dependent on airway epithelial cell-derived IL-25, which induced the upregulation of the notch ligand Jagged1 on dendritic cells. This study identifies a novel pathway underlying viral-driven Th2 responses that may have functional relevance to viral-associated asthma.
Acute Ablation of Langerhans Cells Enhances Skin Immune Responses
Authors: Bobr Aleh , Olvera-Gomez Irlanda , Igyarto Botond Z. , Haley Krystal M. , Hogquist Kristin A. , Kaplan Daniel H. 
Published: September 2010

Abstract
Understanding the function of Langerhans cells (LCs) in vivo has been complicated by conflicting results from LC-deficient mice. Human Langerin-DTA mice constitutively lack LCs and develop exaggerated contact hypersensitivity (CHS) responses. Murine Langerin-diptheria toxin receptor (DTR) mice allow for the inducible elimination of LCs and Langerin+ dermal dendritic cells (dDCs) after administration of diphtheria toxin, which results in reduced CHS. When Langerin+ dDCs have partially repopulated the skin but LCs are still absent, CHS returns to normal. Thus, LCs appear to be suppressive in human Langerin-DTA mice and redundant in murine Langerin-DTR mice. To determine whether inducible versus constitutive LC ablation explains these results, we engineered human Langerin-DTR mice in which diphtheria toxin ablates LCs without affecting Langerin+ dDCs. The inducible ablation of LCs in human Langerin-DTR mice resulted in increased CHS. Thus, LC-mediated suppression does not require their absence during ontogeny or during the steady-state and is consistent with a model in which LCs actively suppress Ag-specific CHS responses.
IL-18 Production Downstream of the Nlrp3 Inflammasome Confers Protection against Colorectal Tumor Formation
Authors: Zaki Mohammad Hasan , Vogel Peter , Body-Malapel Mathilde , Lamkanfi Mohamed , Kanneganti Thirumala-Devi 
Published: September 2010

Abstract
Colorectal cancer is a leading cause of cancer-related deaths worldwide. Chronic inflammation is recognized as a predisposing factor for the development of colon cancer, but the molecular mechanisms linking inflammation and tumorigenesis have remained elusive. Recent studies revealed a crucial role for the NOD-like receptor protein Nlrp3 in regulating inflammation through the assembly of proinflammatory protein complexes termed inflammasomes. However, its role in colorectal tumor formation remains unclear. In this study, we showed that mice deficient for Nlrp3 or the inflammasome effector caspase-1 were highly susceptible to azoxymethane/dextran sodium sulfate-induced inflammation and suffered from dramatically increased tumor burdens in the colon. This was a consequence of markedly reduced IL-18 levels in mice lacking components of the Nlrp3 inflammasome, which led to impaired production and activation of the tumor suppressors IFN-γ and STAT1, respectively. Thus, IL-18 production downstream of the Nlrp3 inflammasome is critically involved in protection against colorectal tumorigenesis.
Research Report: The Lingering Effect of Colonialism on Tourist Movements
Authors: McKercher Bob , Decosta Patrick L'Espoir 
Published: September 2010

This short study examines the lasting effects of colonial ties on tourist movements between nations and their current and former possessions. The study examines 56 jurisdictions in six geographical regions that were once colonies of or remain the possessions of eight nations. Residents of Europe and America show a preference for travel to destinations with strong historical/political ties and an equally strong aversion to travel to jurisdictions where no such connection exists. Likewise, destinations rely heavily on former colonizers as a key source of visitors, especially from long-haul markets. Such a pattern is not evident among either source European markets without a strong colonial history or destination countries without a colonial past. The lingering effects of colonialism are especially noticeable among French and Dutch tourists. The study suggests that ‘colonialism’ may need to be included as a variable in models of tourist movements. colonialism tourism movements
Palestinian suffering: some personal, historical, and psychoanalytic reflections
Authors: Jarrar Adib 
Published: September 2010

AbstractThis paper reminds the “share holders” of the international psychoanalytical enterprise of their collective responsibility towards a major social, national and moral injustice that has been grasping the attention of the remaining world for many decades. This involves the suffering of the Palestinian people who are under a brutal siege of occupation, intimidation, and disenfranchisement. Lacking a world‐endorsed nationhood, threatened in the preservation of their culture, barred from travel, used and abused by Arab regimes, and, often ignored by the international community, the Palestinians strive to save their dignity – sometimes by political praxis and at other times by violent resistance. This paper offers a description of this national/political/humanitarian tragedy in the hope of enhancing knowledge, engendering empathy, and mobilizing reparative action. Copyright © 2010 John Wiley & Sons, Ltd.
An evaluation of self‐reported mobile phone use compared to billing records among a group of engineers and scientists
Authors: Shum Mona , Kelsh Michael A. , Sheppard Asher R. , Zhao Ke 
Published: September 2010

AbstractMost epidemiologic studies of potential health impacts of mobile phones rely on self‐reported information, which can lead to exposure misclassification. We compared self‐reported questionnaire data among 60 participants, and phone billing records over a 3‐year period (2002–2004). Phone usage information was compared by the calculation of the mean and median number of calls and duration of use, as well as correlation coefficients and associated P‐values. Average call duration from self‐reports was slightly lower than billing records (2.1 min vs. 2.8 min, P = 0.01). Participants reported a higher number of average daily calls than billing records (7.9 vs. 4.1, P = 0.002). Correlation coefficients for average minutes per day of mobile phone use and average number of calls per day were relatively high (R = 0.71 and 0.69, respectively, P < 0.001). Information reported at the monthly level tended to be more accurate than estimates of weekly or daily use. Our findings of modest correlations between self‐reported mobile phone usage and billing records and substantial variability in recall are consistent with previous studies. However, the direction of over‐ and under‐reporting was not consistent with previous research. We did not observe increased variability over longer periods of recall or a pattern of lower accuracy among older age groups compared with younger groups. Study limitations included a relatively small sample size, low participation rates, and potential limited generalizability. The variability within studies and non‐uniformity across studies indicates that estimation of the frequency and duration of phone use by questionnaires should be supplemented with subscriber records whenever practical. Bioelectromagnetics 32:37–48, 2011. © 2010 Wiley‐Liss, Inc.
Stabilization of oligomeric structure of α‐crystallin by Zn+2 through intersubunit bridging
Authors: Karmakar Srabani , Das K. P. 
Published: September 2010

Abstractα‐Crystallin, the major protein of mammalian eye lens, is a member of the small heat shock protein family and is a molecular chaperone. We previously reported that its molecular chaperone function as well as stability increased in presence of Zn+2. Despite the effect of Zn+2 on the structure and function of α‐crystallin, evidence for direct interaction between them remained elusive. We now present the MALDI mass spectrometric data that shows direct evidence of Zn+2 binding to recombinant αA‐ and αB‐crystallin. The binding stoichiometry was over three Zn+2 per subunit of α‐crystallin at zinc/protein molar ratio of 20. Observation of multiple Zn+2 binding is consistent with the large increase in thermodynamic stability. Sequence‐based analysis of αA‐ and αB‐crystallin predicted both proteins to be nonzinc binding proteins. Our dynamic light scattering data shows that Zn+2 stabilizes the oligomeric structure of α‐crystallin by bridging neighboring subunits in multiple centers. Despite the low affinity binding, the intersubunit bridging by multiple Zn+2 makes the oligomer so stable that oligomer breakdown does not occur even at 6M urea. The subunit bridging has been supported by our FRET data that showed absence of subunit exchange in presence of zinc. MALDI data also showed that the interaction of α‐crystallin with Zn+2 is quite different from other bivalent metal ions. Bound Zn+2 could be easily removed by dialysis of the complex. The relevance of such weak interaction on the stability of the oligomeric structure of α‐crystallin and its function in the eye lens has been discussed. © 2010 Wiley Periodicals, Inc. Biopolymers 95: 105–116, 2011.
Computer‐aided experimental modeling of a real system using graphical analysis of a step response data
Authors: Furat Murat , Eker İlyas 
Published: September 2010

ABSTRACTIn the present article, a graphical user interface (GUI) application is introduced for undergraduate students to model a real system. The object is to provide experiences on modeling of a real system with the equipment in the control laboratory by using the graphical modeling methods. It is to show the students how a real system is connected to a computer. An experiment is assigned to the students to integrate their theoretical knowledge with their practical skills. Since classical courses are limited to the related theoretical knowledge given by an instructor, the students can gain valuable experiences with the developed GUI. The designed GUI application provides to the students to focus only on modeling methods. It is observed that the experiment increases the students' motivation resulting in enhanced education with respect to the classical education.© 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 22:571–582, 2014; View this article online at wileyonlinelibrary.com/journal/cae; DOI 10.1002/cae.20482
Dimeric Self‐Assembly of Pyridyl Guanidinium Carboxylates in Polar Solvents
Authors: Irfan Ashiq Muhammad , Tesfatsion Biniam F. , Gaggini Francesca , Dixon Sally , Kilburn Jeremy D. 
Published: September 2010

AbstractA series of pyridyl guanidinium–carboxylates has been prepared and the dimeric self‐assembly of these studied in H2O/DMSO mixtures, principally using dilution isothermal calorimetry. Compounds 5 and 6, incorporating an aromatic ring in the “tethering” region between the guanidinium and carboxylate groups, demonstrate the strongest dimerisation in neat DMSO. X‐ray crystal structures of 5 and 6 reveal two different dimerisation architectures in the solid‐state, but both involve carboxylate–guanidinium salt bridges as anticipated, and π–π interactions. Compounds 10–16 incorporating peptidic fragments between the guanidinium and carboxylate groups, showed reduced dimerisation strength with increased amino acid content, but also sustained dimerisation under increasingly aqueous conditions, up to 50 % H2O/DMSO in the case of 14 and 15. The extent of our study in H2O/DMSO mixtures was determined by substrate solubility of 10–16, and not the limit of self‐assembly.
ChemInform Abstract: Crystal Structure Determination of Ammonium Catena‐Polyphosphate II by X‐Ray Powder Techniques.
Authors: BRUEHNE B. , JANSEN M. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Studies of the Spinel Solid Solution Co2Ru1‐xFexO4.
Authors: MENDONCA M. H. , NUNES M. R. , COSTA F. M. A. , CARVALHO A. , GODINHO M. M. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Preparation and Optical Nonlinear Property of Sol‐Gel‐Derived Cu‐SiO2 Thin Films.
Authors: KUNDU D. , HONMA I. , OSAWA T. , KOMIYAMA H. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Effect of BaWO4 on the Microwave Dielectric Properties of Ba(Mg1/3Ta2/ 3)O3 Ceramics.
Authors: YOON K. H. , KIM D. P. , KIM E. S. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: CuInS2 with Lamellar Morphology. Part 1. Efficient Photoanodes in Acidic Polyiodide Medium.
Authors: CATTARIN S. , DIETZ N. , LEWERENZ H. J. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: CuInS2 with Lamellar Morphology. Part 2. Photoelectrochemical Behavior of Heterogeneous Material.
Authors: CATTARIN S. , GUERRIERO P. , RAZZINI G. , LEWERENZ H.‐J. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.