bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dichlorvos, chlorpyrifos oxon and Aldicarb adducts of butyrylcholinesterase, detected by mass spectrometry in human plasma following deliberate overdose
Authors: Li Bin , Ricordel Ivan , Schopfer Lawrence M. , Baud Frédéric , Mégarbane Bruno , Masson Patrick , Lockridge Oksana 
Published: April 2010

AbstractThe goal of this study was to develop a method to detect pesticide adducts in tryptic digests of butyrylcholinesterase in human plasma from patients poisoned by pesticides. Adducts to butyrylcholinesterase in human serum may serve as biomarkers of pesticide exposure because organophosphorus and carbamate pesticides make a covalent bond with the active site serine of butyrylcholinesterase. Serum samples from five attempted suicides (with dichlorvos, Aldicarb, Baygon and an unknown pesticide) and from one patient who accidentally inhaled dichlorvos were analyzed. Butyrylcholinesterase was purified from 2 ml serum by ion exchange chromatography at pH 4, followed by procainamide affinity chromatography at pH 7. The purified butyrylcholinesterase was denatured, digested with trypsin and the modified peptide isolated by HPLC. The purified peptide was analyzed by multiple reaction monitoring in a QTRAP 4000 mass spectrometer. This method successfully identified the pesticide‐adducted butyrylcholinesterase peptide in four patients whose butyrylcholinesterase was inhibited 60–84%, but not in two patients whose inhibition levels were 8 and 22%. It is expected that low inhibition levels will require analysis of larger serum plasma volumes. In conclusion, a mass spectrometry method for identification of exposure to live toxic pesticides has been developed, based on identification of pesticide adducts on the active site serine of human butyrylcholinesterase. Copyright © 2010 John Wiley & Sons, Ltd.
The “Raman spectroscopic signature of life” is closely related to haem function in budding yeasts
Authors: Chiu Liang‐da , Hamaguchi Hiro‐o 
Published: April 2010

AbstractHEM1 gene encodes δ‐aminolevulinate synthase that is required for haem synthesis. It is an essential gene for yeast survival. The Raman spectra of HEM1 knockout (hem1Δ) yeast cells lacks a Raman band at 1602 cm–1 that has been shown to reflect cell metabolic activity. This result suggests that the molecule giving rise to the“Raman spectroscopic signature of life” is closely related to haem functions in the cell. High amount of squalene is also observed in the hem1Δ strain, which is another new discovery of this study. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
The impact of Roux‐en‐Y gastric bypass surgery for morbid obesity on second trimester obstetric ultrasound completion rates
Authors: Wax Joseph R. , Cartin Angelina , Pinette Michael G. , Blackstone Jacquelyn 
Published: April 2010

AbstractPurpose.Weight loss after bariatric surgery occurs by preferential loss of visceral and central body fat. We hypothesized that 2nd trimester sonographic completion rates would be higher among women with prior gastric bypass compared with women of similar body mass index without a prior gastric bypass.Methods.Women with gastric bypass undergoing 2nd trimester sonographic examination were matched 1:1 with controls for body mass index and prior cesarean section. The primary outcome was completion of the fetal anatomic evaluation according to American Institute of Ultrasound in Medicine guidelines.Results.Initial sonogram completion rates for cases, 28/40 (70%), and controls (30/40, 75%) were similar (p = 0.62). Completion rates for women returning for follow‐up were 100% in both groups. Overall completing rates were identical for cases (37/40, 92.5%) and controls (37/40, 92.5%).Conclusion.Previous gastric bypass surgery does not affect 2nd trimester sonographic completion rates. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound, 2010
Human adenovirus replication in immunocompetent Syrian hamsters can be attenuated with chlorpromazine or cidofovir
Authors: Diaconu Iulia , Cerullo Vincenzo , Escutenaire Sophie , Kanerva Anna , Bauerschmitz Gerd J. , Hernandez‐Alcoceba Ruben , Pesonen Sari , Hemminki Akseli 
Published: April 2010

AbstractBackgroundAdenoviruses can cause severe toxicity in children and in immunocompromised adults, and therefore a means to abrogate replication would be useful. With regard to cancer treatment, replication competent oncolytic adenoviruses have been safe in humans, although their efficacy has been variable. Therefore, more effective agents are now entering clinical testing and, consequently, replication‐associated side effects remain a concern. Preclinical analysis of replication related toxicity has been hampered by a lack of permissive models. Therefore, it has been difficult to study modulation of human adenovirus replication in immune competent animals.MethodsWe investigated four different hamster carcinoma cell lines for transduction and cell killing potency in vitro and in vivo. Gene transfer was assessed using replication‐deficient adenoviruses expressing luciferase. Cell killing was studied in vitro and in vivo using an oncolytic adenovirus that kills tumor cells by viral replication. After the most promising animal model had been selected, abrogation of virus replication was assessed in vitro and in vivo using a TCID50 assay.ResultsThe results obtained suggest wild‐type adenovirus replication in all four tested Syrian hamster cell lines and also normal organs. Virus replication could be abrogated with chlorpromazine, cidofovir and cytosine arabinoside, and the effect occurred subsequent to nuclear delivery of the viral genome. Attenuation of virus replication also was seen in vivo both in tumors and the liver.ConclusionsSyrian hamsters may comprise a valuable immune competent model for evaluating anti‐adenoviral drugs. Furthermore, chlorpromazine or cidofovir might be useful in case of adenovirus replication‐associated symptoms in humans. Copyright © 2010 John Wiley & Sons, Ltd.
T1 and T2 hypopharyngeal cancer treatment with laser microsurgery
Authors: Karatzanis Alexander D. , Psychogios George , Waldfahrer Frank , Zenk Johannes , Hornung Joachim , Velegrakis George A. , Iro Heinrich 
Published: April 2010

AbstractBackground and ObjectiveTo assess the oncologic results and functional outcomes of CO2 laser microsurgery in T1 and T2 hypopharyngeal cancer.MethodsThe files of 119 T1 and T2 hypopharyngeal carcinoma cases primarily managed with laser surgery were reviewed. Cases were assessed for 5‐year disease‐specific survival (DSS) as well as local control (LC) rates, with respect to T and N classification, status of surgical margins, and decision on neck management and adjuvant therapy. Cases were additionally evaluated for incidence of major complications and retention of laryngeal and pharyngeal function.ResultsDSS and LC rates of 72.6% and 85.4%, respectively, were noted overall in this series. Survival rates were found to be significantly better for cases with negative surgical margins. The presence of regional metastases was also found to significantly affect prognosis. Satisfactory retention of function and a low rate of major complications were noted.ConclusionLaser surgery appears to be very effective for T1 and T2 hypopharyngeal cancer treatment as long as clear surgical margins can be achieved. Oncologic results are acceptable with low incidence of complications and satisfactory retention of function. The neck must always be included in the primary treatment plan of hypopharyngeal lesions. J. Surg. Oncol. J. Surg. Oncol. 2010;102:27–33. © 2010 Wiley‐Liss, Inc.
Miniemulsions via in situ Surfactant Generation
Authors: El‐Jaby Ula , Cunningham Michael , McKenna Timothy F. L. 
Published: April 2010

AbstractThe preparation and stabilisation of miniemulsions using in situ generated surfactants is described. Neutralising a water‐soluble base with an oil‐soluble long‐chain acid instantaneously generates in situ surfactants at the oil/water interface, resulting in a rapid homogenisation/polymerisation process. It was found that droplet sizes decreased with surfactant concentration and solid content and that in situ generated potassium stearate is not capable of simultaneously serving as a surfactant and costabiliser. However, the presence of stearic acid delays the rate of ripening. A number of in situ surfactants were prepared with different acids and bases. It was found that the droplet size decreased with the hydrated radius of the counterions.magnified image
Neuronal intranuclear inclusion disease: Two cases of dopa‐responsive juvenile parkinsonism with drug‐induced dyskinesia
Authors: Lai Szu‐Chia , Jung Shih‐Ming , Grattan‐Smith Padraic , Sugo Ella , Lin Yen‐Wen , Chen Rou‐Shayn , Chen Chiung‐Chu , Wu‐Chou Yah‐Huei , Lang Anthony E. , Lu Chin‐Song 
Published: April 2010

AbstractThere are very few conditions that present with dopa‐responsive juvenile parkinsonism. We present two such children with neuronal intranuclear inclusion disease (NIID) who had an initial good levodopa response that was soon complicated by disabling dopa‐induced dyskinesia. One child was diagnosed by rectal biopsy in life, and the other diagnosis was confirmed at postmortem. In this patient, dopamine transporter imaging showed severely decreased binding of the radiotracer in the striatum on both sides. Bilateral subthalamic deep brain stimulation in this patient produced initial improvement, but this was not sustained. Both patients died within 10 years of symptom onset. As well as levodopa responsiveness with rapid onset of dyskinesia, clues to the diagnosis of NIID in patients presenting with parkinsonism include the presence of gaze‐evoked nystagmus, early onset dysarthria and dysphagia and oculogyric crises. Differential diagnosis of clinical symptoms and neuropathological findings are discussed including the approach to rectal biopsy for early diagnosis. © 2010 Movement Disorder Society
Clinical efficacy of istradefylline (KW‐6002) in Parkinson's disease: A randomized, controlled study
Authors: Mizuno Yoshikuni , Hasegawa Kazuko , Kondo Tomoyoshi , Kuno Sadako , Yamamoto Mitsutoshi ,   The Japanese Istradefylline Study Group
Published: April 2010

AbstractThe objectives of this study were to evaluate the efficacy of istradefylline at an oral dose of 20 mg or 40 mg once daily for 12 weeks in Parkinson's disease (PD) patients with motor complications on levodopa therapy based on the change in the daily OFF time compared with placebo and to assess the safety at these doses. A total of 363 subjects were randomly assigned to receive 20 mg/day istradefylline (n = 119), 40 mg/day istradefylline (n = 125), or placebo (n = 119). The primary outcome variable was the change from baseline at endpoint in daily OFF time based on patients' ON/OFF diaries. At endpoint, the daily OFF time reduced from baseline by 1.31 hours for 20 mg/day istradefylline (P = 0.013 as compared to the placebo), 1.58 hours for 40 mg/day istradefylline (P < 0.001), and 0.66 hours for placebo; istradefylline significantly reduced the daily OFF time compared with placebo. The UPDRS Part III subscale score (ON state) reduced by 5.7 at endpoint in both istradefylline groups and 3.7 in the placebo group (P = 0.006 for 20 mg/day and P = 0.006 for 40 mg/day group as compared with placebo). The most commonly reported drug‐related treatment emergent adverse event (TEAE) was dyskinesia, which occurred in 2.5% (3/119) of subjects receiving placebo, 8.5% (10/118) receiving 20 mg/day istradefylline, and 6.4% (8/125) receiving 40 mg/day istradefylline. We conclude that istradefylline at 20 mg and 40 mg once daily is effective in relieving wearing‐off fluctuations of PD patients. In addition, istradefylline was well tolerated at both doses. © 2010 Movement Disorder Society
3D‐QSAR CoMFA Study to Predict Orientation of Suprahistaprodifens and Phenoprodifens in the Binding‐Pocket of Four Histamine H1‐Receptor Species
Authors: Straßer Andrea , Wittmann Hans‐Joachim 
Published: April 2010

AbstractSeveral phenylhistamines and histaprodifens were identified to act as (partial) agonists at the histamine H1‐receptor (H1R). The large diversity of these compounds led to pKivalues in a range between 4.5–7.5 at human, rat, bovine and guinea‐pig histamine H1R. Hybrid molecules, consisting of two different H1R‐agonist partial structures, like suprahistaprodifens or phenoprodifens for example, or compounds with a dimeric histamine moiety are able to bind in two different orientations into the binding‐pocket of the H1R. In order to predict the percentage of ligand bound in orientation 1 or in orientation 2, 3D‐QSAR studies at four H1R species were performed. The training set contains ligands which can only bind in one orientation and the test set all ligands with two possible orientations. In general, the predicted pKivalues of the test compounds were in good correlation to the experimental ones. Additionally, the predicted pKivalues for all ligands with two possible orientations were used to calculate the percentage of the hybrid ligands bound in orientation 1 and 2. Our models suggest that the preferred orientation of these hybrid molecules is dependent on ligand and H1R species.
NMR analysis of a series of imidazobenzoxazines
Authors: Rivara Mirko , Fantini Marco , Acquotti Domenico , Zuliani Valentina 
Published: April 2010

AbstractThe complete 1H and 13C NMR assignment of a series of imidazobenzoxazines by a combination of one‐ and two‐dimensional experiments (COSY, HSQC and HMBC) is studied. Moreover, 2D NOESY and 1D selective NOESY are reported. This procedure allows the identification of the regioisomers obtained. Copyright © 2010 John Wiley & Sons, Ltd.
The diffusion NMR studies of crown ethers–cyclodextrin complexation process
Authors: Gierczyk Błażej , Nowak‐Wydra Barbara , Schroeder Grzegorz , Pankiewicz Radosław 
Published: April 2010

AbstractFormation of complexes between crown ethers and cyclodextrins has been studied using diffusion measurements. The structures of the complexes have been proposed on the basis of the theoretical calculations and the results obtained. Relation between the structural parameters (rings' diameter and symmetry) and the tendency of molecules' interactions are discussed. Copyright © 2010 John Wiley & Sons, Ltd.
Do fall predictors in middle aged and older adults predict fall status in persons 50+ with fibromyalgia? An exploratory study
Authors: Rutledge Dana N. , Cherry Barbara J. , Rose Debra J. , Rakovski Carter , Jones C. Jessie 
Published: April 2010

AbstractWe explored potential predictors of fall status in 70 community‐dwelling persons ≥50 years of age with fibromyalgia (FM). Over 40% of the sample reported one or more falls in the year prior to the study. A logistic regression model using 10 variables known to predict falls in middle aged and older persons predicted 45% of the variance in fall status. Three variables offered significant independent contributions to the overall model predicting fall status: perception of postural instability, balance performance, and executive function processing speed. The results support prior work in both nonclinical and clinical populations of middle aged and older adults indicating that falls are associated with multiple risk factors. Prospective designs with larger samples are needed to (a) validate and extend these findings, and (b) identify risk factors related to fall status that are unique to persons with FM. © 2010 Wiley Periodicals, Inc. Res Nurs Health 33:192–206, 2010
Synthesis of high oil‐absorption resins of poly(methyl methacrylate‐butyl methacrylate) by suspended emulsion polymerization
Authors: Ji Naiyi , Chen Hou , Yu Mengmeng , Qu Rongjun , Wang Chunhua 
Published: April 2010

AbstractThe synthesis of high oil‐absorption resins by suspended emulsion polymerization process for the first time with butyl methacrylate (BMA) and short‐chain methyl methacrylate (MMA) as the monomers was studied. And the effects of different polymerization technological parameters, such as the comonomer, initiator, crosslinker, emulsifier, dispersant agent, and the agitation rate, on the oil absorbency of high oil‐absorption resins were discussed in detail. The optimum polymerization conditions were obtained. With the increasing contents of these factors, the oil absorbency increased first, and then decreased. The highest oil absorbency to toluene was 17.6 g/g. The particle morphology of the high oil‐absorption resins was observed by scanning electron microscopy (SEM). The resins were determined by FTIR spectrometry. Compared with the high oil‐absorption resins prepared by suspension polymerization process and emulsion polymerization process, the high oil‐absorption resins prepared by suspended emulsion polymerization process had the higher oil absorbency, faster oil‐adsorbing rate, better oil‐retention, and regeneration property. Copyright © 2010 John Wiley & Sons, Ltd.
Tigecycline treatment of multi‐drug‐resistant Corynebacterium jeikeium infection in a child with relapsing and refractory acute lymphoblastic leukemia
Authors: Dinleyici Ener Cagri , Yargic Zeynel Abidin , Bor Ozcan , Kiremitci Abdurrahman , Durmaz Gul 
Published: April 2010

AbstractCorynebacterium jeikeium has been recognized as an important cause of infection, particularly among neutropenic patients who have central venous catheter (CVC). Routine use of tigecycline in children is not yet approved. Here in we present a child with relapsed‐refractory lymphoblastic leukemia who was successfully treated with tigecyline due to multi‐drug‐resistant C. jeikeium sepsis without removal of CVC. Our case highlights the use of tigecycline where there are no alternatives. Further studies regarding the efficacy and safety of tigecycline in pediatric patients are needed. Pediatr Blood Cancer. 2010;55:349–351. © 2010 Wiley–Liss, Inc.
Association among lung function, exhaled nitric oxide, and the CAN questionnaire to assess asthma control in children
Authors: Sardón‐Prado O. , Korta‐Murua J. , Valverde‐Molina J. , Fernández‐Paredes J.J. , Mintegui J. , Corcuera‐Elosegui P. , Emparanza J.I. , Pérez‐Yarza E.G. 
Published: April 2010

AbstractBackgroundThe aim of this study was to investigate the association among a validated symptom‐based questionnaire for asthma control in children (CAN), forced expiratory volume in 1 sec (FEV1), and fractional exhaled nitric oxide (FENO).MethodsObservational cross‐sectional study was performed in a consecutive sample of asthmatic children aged between 7 and 14 years old from December 2007 to February 2008. FENO was measured with a portable electrochemical analyzer and forced spirometry was performed according to American Thoracic Society/European Respiratory Society. The CAN questionnaire was completed by the parents (aged <9 years old) or by the children (≥9 years old). The strength of the association among FEV1, FENO, and CAN questionnaire was studied using Spearman's rho, and the degree of agreement for asthma control among FEV1, FENO, and CAN questionnaire, with classification of these variables according to values of normality, was studied using Pearson's χ2 test and Cohen's kappa (KC).ResultsWe studied 268 children, mean age 9.7 ± 2.1 years. Significant correlations were found between FENO and CAN (r = 0.2), between FEV1 and CAN (r = −0.3), and between FENO and FEV1 (r = −0.12). On classifying the variables according to values of normality, no agreement was found to establish the degree of asthma control between FENO and CAN (KC = 0.18, χ2 Pearson = 9.63); between FEV1 and CAN (KC = 0.29, χ2 = 38.5); or between FENO and FEV1 (KC = 0.07, χ2 = 4.9).ConclusionsThe association among the three measurement instruments used to assess asthma control (FEV1, FENO, and CAN) was weak. These are instruments that quantify variables that influence asthma in different ways, in this sense, none can be used instead of another in asthma management although they are complementary. Pediatr Pulmonol. 2010; 45:434–439. © 2010 Wiley‐Liss, Inc.
Lung function testing in preschool‐aged children with cystic fibrosis in the clinical setting
Authors: Gangell Catherine L. , Hall Graham L. , Stick Stephen M. , Sly Peter D. 
Published: April 2010

AbstractIn cystic fibrosis (CF) lung function testing is a means of monitoring progression of lung disease. The preschool years have often been referred to as the “silent years” due to the previous lack suitable measures of lung function testing in this age group. This review outlines the various techniques of lung function testing in preschool children with CF in the clinical setting. This includes measures requiring tidal breathing including the forced oscillation technique, the interrupter technique, plethysmography, and multiple breath washout, as well as spirometry that requires respiratory maneuvers. We describe the feasibility and variability of different lung function methods used in preschoolers and report measurements made during tidal breathing have greater feasibility, although greater variability compared to spirometry. We also report associations with lung function and markers of CF lung disease. In the preschool age group measurements made during tidal breathing may be more appropriate in the clinic setting than those that require a higher degree of cooperation and specific respiratory maneuvers.maneuvers. Pediatr Pulmonol. 2010; 45:419–433. © 2010 Wiley‐Liss, Inc.
A computational study of RNA binding and specificity in the tandem zinc finger domain of TIS11d
Authors: Morgan Brittany R. , Massi Francesca 
Published: April 2010

AbstractTIS11d is a member of the CCCH‐type family of tandem zinc finger (TZF) proteins; the TZF domain of TIS11d (residues 151–220) is sufficient to bind and destabilize its target mRNAs with high specificity. In this study, the TZF domain of TIS11d is simulated in an aqueous environment in both the free and RNA‐bound states. Multiple nanosecond timescale molecular dynamics trajectories of TIS11d wild‐type and E157R/E195K mutant with different RNA sequences were performed to investigate the molecular basis for RNA binding specificities of this TZF domain. A variety of measures of the protein structure, fluctuations, and dynamics were used to analyze the trajectories. The results of this study support the following conclusions: (1) the structure of the two fingers is maintained in the free state but a global reorientation occurs to yield a more compact structure; (2) mutation of the glutamate residues at positions 157 and 195 to arginine and lysine, respectively, affects the RNA recognition by this TIS11d mutant in agreement with the findings of Pagano et al. (J Biol Chem 2007; 282:8883–8894); and (3) we predict that the E157R/E195K mutant will present a more relaxed RNA binding specificity relative to wild‐type TIS11d based on the more favorable nonsequence‐specific Coulomb interaction of the two positively charged residues at positions 157 and 195 with the RNA backbone, which compensates for a partial loss of the stacking interaction of aromatic side chains with the RNA bases.
Structural requirements for repellency: norsesquiterpenes and sesquiterpenoid derivatives of nootkatone against the Formosan subterranean termite (Isoptera: Rhinotermitidae)
Authors: Zhu Betty CR , Henderson Gregg , Sauer Anne M , Crowe William , Laine Roger A 
Published: April 2010

AbstractBACKGROUND:Research has shown that the family of grapefruit flavors called nootkatones have significant repellant and toxic effects to Formosan subterranean termites (Coptotermes formosanus Shiraki).RESULTS:Nineteen synthetic nootkatone derivatives, along with three commercially available nootkatone derivatives, were tested for repellent activity against C. formosanus by a choice assay in a petri dish with a two‐step triage procedure. Based on the repellency threshold value, the relationships between structure and activity are discussed.CONCLUSION:Four derivatives of nootkatone have very high repellency and toxicity to C. formosanus, 9 times the potency of the primary compound nootkatone. Four other compounds have between 2 and 3 times the repellency of nootkatones, and three compounds are equal in their repellency to nootkatone. Copyright © 2010 Society of Chemical Industry