bluuebunny/crossref_metadata_2025_split huggingface dataset:
Systemic lupus erythematosus complicated with posterior reversible encephalopathy syndrome and intracranial vasculopathy
Authors: CHEN Hung‐An , LIN Yeong‐Jang , CHEN Pei‐Chih , CHEN Tai‐Yuan , LIN Kao‐Chang , CHENG He‐Hsiung 
Published: June 2010

AbstractPosterior reversible encephalopathy syndrome (PRES) is a neurotoxic condition characterized by reversible vasogenic edema on neuroimaging. It is associated with various neurological manifestations, including headaches, vomiting, seizures, visual loss, altered mental status and focal neurological deficits. PRES mainly occurs in the setting of eclampsia, hypertension, uremia, malignancy, transplantation, autoimmune diseases and/or use of immunosuppressive drugs. This syndrome has been described in patients with systemic lupus erythematosus (SLE). PRES is a potentially reversible clinical–radiological entity; however, it can be complicated with vasculopathy, infarction or hemorrhage. Vasculopathy has been demonstrated to be a common finding in patients with SLE. We report the case of a woman with lupus nephritis and PRES whose diffuse vasculopathy was present on initial neuroimaging. Subsequent brain computed tomography scan demonstrated interval development of intraparenchymal hemorrhage and subarachnoid hemorrhage. To our knowledge, this unique brain image pattern has not been reported in SLE patients.
On nearby cycles and 𝒟-modules of log schemes in characteristic p>0
Authors: Tsuji Takeshi 
Published: June 2010

AbstractLet K be a complete discrete valuation field of mixed characteristic (0,p) with a perfect residue field k. For a semi-stable scheme over the ring of integers OK of K or, more generally, for a log smooth scheme of semi-stable type over k, we define nearby cycles as a single 𝒟-module endowed with a monodromy ∂logt, whose cohomology should give the log crystalline cohomology. We also explicitly describe the monodromy filtration of the 𝒟-module with respect to the endomorphism ∂logt, and construct a weight spectral sequence for the cohomology of the nearby cycles.
Effect of Electroacupuncture on the Healing Process of Tibia Fracture in a Rat Model: A Randomised Controlled Trial
Authors: Nakajima Miwa , Inoue Motohiro , Hojo Tatsuya , Inoue Nozomu , Tanaka Kazuto , Takatori Ryota , Itoi Megumi 
Published: June 2010

Background Electrical stimulation is used to promote bone reunion, and is most effective when applied directly to the fracture site. Objective To examine the effects of electroacupuncture (EA) on the healing process of tibia fracture in a rat model. Methods Thirty 12-week-old male Wistar rats underwent unilateral open osteotomies of the tibiae. The rats were then assigned randomly to three groups: EA group (n=10), sham group (n=10) and control group (n=10). In the EA group, a cathodal electrode was connected to an acupuncture needle percutaneously penetrated directly at the surgery site, while an acupuncture needle inserted at 15 mm proximal to the surgery site was used as an anodal electrode. EA (50 Hz, 20 μA, 20 min) was performed daily for 3 weeks. In the sham group the acupuncture needles were inserted at the same sites but no electrical stimulation was given and in the control group, no treatment was given. The response was evaluated at 1, 3, 4 and 6 weeks after surgery by radiographic, macroscopic and mechanical examinations. Results The EA group showed accelerated bone healing (EA group 29.92±4.55 mm2, sham group 26.46±5.21 mm2, control group 26.19±2.81 mm2, p<0.05 at 3 weeks) and accretion of the callus (radiographic evaluation: EA group 35.66±4.37 mm2, sham group 32.60±5.73 mm2, control group 29.72±6.39 mm2, p<0.05 at 6 weeks) compared with the other groups. Mechanical testing also showed an excellent result (EA group 16.54±9.92 N, sham group 7.13±3.57 N, control group 6.67±3.12 N, p<0.05) at 6 weeks in the EA group compared with the other groups. There was no difference between the sham and control groups in any evaluation. Conclusion The use of EA enhanced callus development and bone mineralisation during the bone healing process.
Immediate Effect of Acupuncture on the Sleep Pattern of Patients with Obstructive Sleep Apnoea
Authors: Freire Anaflávia O , Sugai Gisele C M , Togeiro Sônia Maria , Mello Luiz Eugênio , Tufik Sérgio 
Published: June 2010

Background Most patients with obstructive sleep apnoea (OSA) do not tolerate treatment with nasal continuous positive airway pressure, the ‘gold standard’ treatment for this condition. It was shown in a pilot study that acupuncture was more effective than placebo treatment (sham acupuncture) in producing significant changes in the respiratory events assessed by polysomnography (PSG). Objectives To investigate the immediate effect of manual acupuncture (MA) and electroacupuncture (EA) on the sleep pattern of patients presenting with moderate OSA. Methods 40 patients with an Apnoea–Hypopnoea Index (AHI) of 15–30/h were randomly allocated to MA treatment (n=10), EA 10 Hz treatment (n=10), EA 2 Hz treatment (n=10) and a no-treatment control group (n=10). The patients received MA or EA (2 or 10 Hz) just before the PSG study at 20:00. Results The AHI (p=0.005; p=0.005), the Apnoea Index (p=0.038; p=0.009) and the respiratory events (p=0.039; p=0.014) decreased significantly in the MA and EA 10 Hz groups, respectively (AHI (21.9, 11.2), Apnoea Index (5.15, 0.7), respiratory events (120.5, 61.0) in the MA group before and after. AHI (20.6, 9.9), Apnoea Index (8.2, 0.3), respiratory events (117.0, 56.0) in the EA 10 Hz group before and after). The micro-arousals decreased only in the MA group (146.0 vs 88.5, p=0.0002). There were no significant changes in the EA 2 Hz group or in the control group. Conclusion A single session of either MA or EA 10 Hz had an acute effect in reducing the AHI as well as the number of nocturnal respiratory events of patients presenting with moderate OSA.
Compliance with a comprehensive warm-up programme to prevent injuries in youth football
Authors: Soligard Torbjørn , Nilstad Agnethe , Steffen Kathrin , Myklebust Grethe , Holme Ingar , Dvorak Jiri , Bahr Roald , Andersen Thor Einar 
Published: June 2010

BackgroundParticipants' compliance, attitudes and beliefs have the potential to influence the efficacy of an intervention greatly.ObjectiveTo characterise team and player compliance with a comprehensive injury prevention warm-up programme for football (The 11+), and to assess attitudes towards injury prevention among coaches and their association with compliance and injury risk.Study DesignA prospective cohort study and retrospective survey based on a cluster-randomised controlled trial with teams as the unit of randomisation.MethodsCompliance, exposure and injuries were registered prospectively in 65 of 125 football teams (1055 of 1892 female Norwegian players aged 13–17 years and 65 of 125 coaches) throughout one football season (March–October 2007). Standardised telephone interviews were conducted to assess coaches' attitudes towards injury prevention.ResultsTeams completed the injury prevention programme in 77% (mean 1.3 sessions per week) of all training and match sessions, and players in 79% (mean 0.8 sessions per week) of the sessions they attended. Compared with players with intermediate compliance, players with high compliance with the programme had a 35% lower risk of all injuries (RR 0.65, 95% CI 0.46 to 0.91, p=0.011). Coaches who had previously utilised injury prevention training coached teams with a 46% lower risk of injury (OR 0.54, 95% CI 0.33 to 0.87, p=0.011).ConclusionsCompliance with the injury prevention programme was high, and players with high compliance had significantly lower injury risk than players with intermediate compliance. Positive attitudes towards injury prevention correlated with high compliance and lower injury risk.
An assessment of chromosomal rearrangements in neopolyploids ofLiliumhybrids
Authors: Xie Songlin , Khan Nadeem , Ramanna M. S. , Niu Lixin , Marasek-Ciolakowska Agnieszka , Arens Paul , van Tuyl Jaap M. 
Published: June 2010

Two types of newly induced polyploids (neopolyploids) of Lilium hybrids were monitored for the occurrence of chromosomal rearrangements through genomic in situ hybridization (GISH) technique. One of the populations was obtained through crossing an allotriploid Longiflorum × Oriental hybrid (LLO) with an allotetraploid Longiflorum × Trumpet hybrid (LLTT), both of which were derived from somatic chromosome doubling. The other type of allopolyploid population was derived from meiotic chromosome doubling in which numerically unreduced (2n) gametes from two different interspecific hybrids, namely, Longiflorum × Asiatic (LA) and Oriental × Asiatic (OA), were used to get backcross progeny with the Asiatic parents. GISH clearly discriminated the three constituent genomes (L, T, and O) in the complements of the progeny obtained from mitotic chromosome doubling. A total of 26 individuals were analyzed from this population and there was no evidence of chromosomal rearrangements. However, in the case of meiotically doubled allopolyploid progeny, considerable frequencies of chromosomal rearrangements were observed through GISH. The so-called chromosomal rearrangements in meiotic polyploids are the result of homoeologous recombination rather than translocations. Furthermore, evidence for the occurrence of meiotic recombination in the LA hybrids has been confirmed with GISH on meiotic chromosomes. Thus, there was evidence that neopolyploids of Lilium hybrids did not possess any noticeable chromosome rearrangements.
Early-generation germplasm introgression from Sorghum macrospermum into sorghum (S. bicolor)
Authors: Kuhlman Les C. , Burson Byron L. , Stelly David M. , Klein Patricia E. , Klein Robert R. , Price H. J. , Rooney William L. 
Published: June 2010

Sorghum has been improved by public and private breeding programs utilizing germplasm mostly from within the species Sorghum bicolor . Recently, hybridization with an Australian species, S. macrospermum (AAB1B1YYZZ), has been demonstrated and the genomic relationship to S. bicolor (AAB1B1) shown to be partially compatible. For this species to be potentially useful to sorghum improvement programs, there must be documented introgression into an S. bicolor background. Fifteen BC1F1 progeny were recovered using the interspecific hybrid as a female and embryo rescue. In these progeny, chromosome numbers ranged from 35 to 70 and all were essentially male-sterile. Repeated backcrossing with S. bicolor pollen produced BC2F1 seed on 3 of the 15 BC1F1 plants. BC2F1 progeny had varying levels of male fertility; selfed seed set ranged from 0% to 95%, with only 2 individuals being completely male-sterile. Using AFLP and SSR markers, genomic introgression of S. macrospermum ranged from 0% to 18.6%. Cytogenetic analysis of 19 individuals revealed that chromosome numbers were 20, except for a single backcross that had 21 chromosomes. Molecular cytogenetic analysis confirmed the presence of recombinant introgression chromosomes as well as alien addition and alien substitution chromosomes within the BC2F1s.
Effects of raloxifene treatment on the phenotype of blood monocytes
Authors: Boudjeltia Karim Zouaoui , Durez Patrick , Oberweis Didier , Guillaume Michel , Remacle Claude , Cauchie Philippe , Vanhaeverbeek Michel , Brohée Dany , Ducobu Jean , Gregoir Catherine 
Published: June 2010

Raloxifene (RLX), a selective oestrogen receptor modulator, has oestrogen-agonist effects on bone, lipoproteins, and homocysteine and oestrogen-antagonist activity in the breast and uterus, positioning it as a potential drug for long-term prevention of coronary heart disease in postmenopausal women. To further evaluate its influence on cardiovascular risk factors, we studied the effects of 60 mg/day RLX on serum lipid levels, inflammatory (high-sensitivity C-reactive protein, and coagulation (fibrinogen) markers, monocytes, and fibrinolysis in 15 healthy postmenopausal women. Markers were measured at baseline, after 1 month without treatment, and after 3 months of treatment. Fibrinolysis was evaluated using the euglobulin clot lysis time (ECLT) determined with a new semiautomatic optical method. Monocyte phenotype was determined by measurement of the expression of the antigens CD14, HLA-DR, and CD62-L using flow cytometry. After 3 months of RLX treatment, we observed a decrease in total cholesterol (p = 0.002), in low-density lipoprotein cholesterol (p <0.001), and in lipoprotein A (p = 0.01). Fibrinogen (p = 0.002) decreased significantly, and high-sensitivity C-reactive protein had a tendency to decrease, but this did not reach statistical significance (p = 0.06). RLX treatment had no effect on ECLT (p = 0.223) or on white blood cell, lymphocyte, and total monocyte counts (p = 0.313). Monocyte expression of HLA-DR, CD14, and CD62-L was not modified by the treatment. In conclusion, we confirm that RLX has beneficial short-term effects on levels of lipids and inflammatory markers, with no effect on fibrinolysis or monocyte phenotype.
Regulation of IRS-2 signaling by IGF-1 receptor in the diabetic rat heart
Authors: Jiang Youde , Steinle Jena J. 
Published: June 2010

Cardiovascular disease involves changes in inflammatory markers. Since insulin/insulin-like growth factor 1 receptor (IGF-1R) can activate vascular endothelial growth factor to promote vascular growth, reduced IGF-1R signaling in the type I diabetic heart could be detrimental, leading to reduced, collateral blood vessel growth. This study assessed whether diabetes can induce an inflammatory phenotype to regulate molecules in the IGF-1 signaling cascade, thus mediating apoptosis. Rats were made diabetic using streptozotocin (to render them type I diabetic) for 2 months with no insulin treatment. At 2 months, rats were sacrificed under anesthesia, and the left ventricle was immediately removed and placed into cold lysis buffer for protein analyses. Western blotting, immunoprecipitation, and enzyme-linked immunosorbent assay analyses were completed to evaluate protein levels. Diabetes increased TNF-α, interleukin-6 (IL-6), and IL-1α levels in the heart. JNK and p42/p44 activity was significantly increased in the diabetic heart, while IGF-1R phosphorylation, IRS-2 tyrosine phosphorylation, and Akt activities were reduced. A significant increase in Bad protein levels and the cleavage of caspase 3 was observed in the diabetic heart. These results suggest that diabetes activates multiple inflammatory markers in the heart, which then signal a decrease in the activities of key players in the insulin-signaling cascade, namely IGF-1R, IRS-2, and Akt, to regulate apoptosis.
Oculohypotensive effect of perindopril in acute and chronic models of glaucoma in rabbits
Authors: Mehta Anita , Iyer Laxmi , Parmar Sanjay , Shah Gaurang , Goyal Ramesh 
Published: June 2010

We studied the effect of perindopril (1%) on intraocular pressure (IOP) and compared it with the effect of pilocarpine, a therapeutic agent used in experimentally induced acute and chronic models of glaucoma in rabbits. Acute glaucoma was induced by intravenous administration of 5% glucose. Pretreatment with topical perindopril (1%) and pilocarpine (1%) prevented acute rise in IOP induced by intravenous administration of 5% glucose. For inducing chronic ocular hypertension in rabbits, 50 units of freshly prepared α-chymotrypsin in 0.1 mL of sterile saline was injected in the posterior chamber of the eye. Perindopril (1%) (35 ± 1.38 mm Hg to 22.45 ± 1.42 mm Hg) and pilocarpine (1%) (34.4 ± 0.81 mm Hg to 20.15 ± 0.69 mm Hg) produced a significant fall in IOP in these rabbits; pretreatment with indomethacin (prostaglandin synthesis inhibitor) did not affect the IOP-lowering action of perindopril (1%). Perindopril (2.71 × 10−7 mol/L) and neostigmine (1.49 × 10−7mol/L) inhibited true cholinesterase and pseudocholinesterase enzyme activity in blood. The cholinesterase enzyme inhibition by perindopril was comparable with that by neostigmine. In conclusion, our data suggest that perindopril reduced IOP in experimentally induced acute and chronic glaucoma in rabbits. One of the possible mechanisms of perindopril, apart from the inhibition of angiotensin-converting enzyme, may be inhibition of the enzyme cholinesterase.
Extensive autolytic fragmentation of membranous versus cytosolic calpain following myocardial ischemia–reperfusion
Authors: Gilchrist James S.C. , Cook Tom , Abrenica Bernard , Rashidkhani Babak , Pierce Grant N. 
Published: June 2010

We investigated calpain activation in the heart during ischemia–reperfusion (I–R) by immunologically mapping the fragmentation patterns of calpain and selected calpain substrates. Western blots showed the intact 78 kDa large subunit of membrane-associated calpain was autolytically fragmented to 56 and 43 kDa signature immunopeptides following I–R. Under these conditions, the 78 kDa calpain large subunit from crude cytosolic fractions was markedly less fragmented, with only weakly stained autolytic peptides detected at higher molecular weights (70 and 64 kDa). Western blots also showed corresponding calpain-like degradation products (150 and 145 kDa) of membrane-associated α-fodrin (240 kDa) following I–R, but in crude myofibrils α-fodrin degradation occurred in a manner uncharacteristic of calpain. For control hearts perfused in the absence of ischemia, autolytic fragmentation of calpain and calpain-like α-fodrin degradation were completely absent from most subcellular fractions. The exception was sarcolemma-enriched membranes, where significant calpain autolysis and calpain-like α-fodrin degradation were detected. In purified sarcoplasmic reticulum membranes, RyR2 and SERCA2 proteins were also highly degraded, but for RyR2 this did not occur in a manner characteristic of calpain. When I–R-treated hearts were perfused with peptidyl calpain inhibitors (ALLN or ALLM; 25 µmol/L), calpain autolysis and calpain-like degradation of α-fodrin were equally attenuated by each inhibitor. However, only ALLN protected against early loss of developed pressure in hearts following I–R, with no functionally protective effect of ALLM observed. Our studies suggest calpain is preferentially activated at membranes following I–R, possibly contributing to impaired ion channel function implicated by others in I–R injury.
Transformation of abandoned farm fields into coniferous plantations: Is there enough vegetation structure left to maintain winter habitat of snowshoe hares?
Authors: Roy C. , Imbeau L. , Mazerolle M. J. 
Published: June 2010

Natural forests will likely be unable to sustainably fulfill society needs for wood fibers and intensively managed plantations could be an alternative source of timber in the future. Abandoned farm fields are often targeted for conversion, as they are already disturbed sites; however, they also represent high-quality habitat for species such as snowshoe hares ( Lepus americanus Erxleben 1777), a keystone mammal in the boreal forest. We evaluated the effect of converting abandoned farm fields (n = 22) to conifer plantations (n = 19) on habitat use by snowshoe hares, using pellet counts and snow-tracking surveys. Both survey techniques yielded similar results: winter habitat use by hares is mostly affected by vegetation cover rather than habitat type. In the short term, plantations do not offer less protective cover than the one found in abandoned farm fields. However, upon reaching a certain height (≥7 m), plantations are mechanically pruned and lose their protective quality. Promoting silvicultural techniques that maintain lateral cover beyond a critical threshold (70%) could preserve the quality of hare habitat for an extended proportion of rotation time of the plantation.
Essential Requirement for PP2A Inhibition by the Oncogenic Receptor c-KIT Suggests PP2A Reactivation as a Strategy to Treat c-KIT+ Cancers
Authors: Roberts Kathryn G. , Smith Amanda M. , McDougall Fiona , Carpenter Helen , Horan Martin , Neviani Paolo , Powell Jason A. , Thomas Daniel , Guthridge Mark A. , Perrotti Danilo , Sim Alistair T.R. , Ashman Leonie K. , Verrills Nicole M. 
Published: June 2010

Abstract
Oncogenic mutations of the receptor tyrosine kinase c-KIT play an important role in the pathogenesis of gastrointestinal stromal tumors, systemic mastocytosis, and some acute myeloid leukemias (AML). Although juxtamembrane mutations commonly detected in gastrointestinal stromal tumor are sensitive to tyrosine kinase inhibitors, the kinase domain mutations frequently encountered in systemic mastocytosis and AML confer resistance and are largely unresponsive to targeted inhibition by the existing agent imatinib. In this study, we show that myeloid cells expressing activated c-KIT mutants that are imatinib sensitive (V560G) or imatinib resistant (D816V) can inhibit the tumor suppressor activity of protein phosphatase 2A (PP2A). This effect was associated with the reduced expression of PP2A structural (A) and regulatory subunits (B55α, B56α, B56γ, and B56δ). Overexpression of PP2A-Aα in D816V c-KIT cells induced apoptosis and inhibited proliferation. In addition, pharmacologic activation of PP2A by FTY720 reduced proliferation, inhibited clonogenic potential, and induced apoptosis of mutant c-KIT+ cells, while having no effect on wild-type c-KIT cells or empty vector controls. FTY720 treatment caused the dephosphorylation of the D816V c-KIT receptor and its downstream signaling targets pAkt, pSTAT5, and pERK1/2. Additionally, in vivo administration of FTY720 delayed the growth of V560G and D816V c-KIT tumors, inhibited splenic and bone marrow infiltration, and prolonged survival. Our findings show that PP2A inhibition is essential for c-KIT–mediated tumorigenesis, and that reactivating PP2A may offer an attractive strategy to treat drug-resistant c-KIT+ cancers. Cancer Res; 70(13); 5438–47. ©2010 AACR.
pp60c-Src Phosphorylates and Activates Vacuolar Protein Sorting 34 to Mediate Cellular Transformation
Authors: Hirsch Dianne S. , Shen Yi , Dokmanovic Milos , Wu Wen Jin 
Published: June 2010

Abstract
Vacuolar protein sorting 34 (VPS34) contributes to the regulation of the mammalian target of rapamycin complex 1/S6 kinase 1 pathway downstream of nutrient signaling. However, intracellular mechanisms leading to VPS34 activation remain unclear. Here, we report that Src directly phosphorylates VPS34, and that this phosphorylation activates VPS34 lipid kinase activity, leading to Src-Y527F–mediated cellular transformation. Silencing endogenous VPS34 specifically inhibits Src-Y527F–induced colony formation in soft agar, but not Ras-G12V–induced colony formation. We have identified two novel hVPS34 mutations, which either eliminate lipid kinase activity (kinase-dead mutant) or reduce tyrosine phosphorylation by Src-Y527F. When kinase-dead mutant of hVPS34 is stably expressed in Src-Y527F–transformed cells, transformation activities are blocked, indicating that the lipid kinase activity of hVPS34 is essential for Src-mediated cellular transformation. Furthermore, stable expression of this hVPS34 kinase-dead mutant causes an increased number of binucleate and multinucleate cells, suggesting that the kinase activity of hVPS34 is also required for cytokinesis. Moreover, when the hVPS34 mutant that has reduced tyrosine phosphorylation by Src is stably expressed in Src-Y527F–transformed cells, Src-Y527F–stimulated colony formation is also reduced. Data presented here provide important evidence that VPS34 lipid kinase activity could be positively regulated by Src-mediated tyrosine phosphorylation in mammalian cells. This finding highlights a previously unappreciated relationship between VPS34, a class III phosphatidylinositol-3-kinase, and Src non–receptor tyrosine kinase. Additionally, we find that the levels of VPS34 expression and tyrosine phosphorylation are correlated with the tumorigenic activity of human breast cancer cells, indicating that Src to VPS34 signaling warrants further investigation as a pathway contributing to the development and progression of human cancers. Cancer Res; 70(14); 5974–83. ©2010 AACR.