bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identificación de E. coli / Coliformes en las ensaladas cocinadas que se expenden en el mercado “9 de Octubre”, Cuenca, agosto 2023
Authors: Benavidez Llanos Natalia Elizabeth , Chicaiza Guanga Ximena Alexandra , Torres Segarra Silvia Monserrath 
Published: February 2025

Introducción: La ingesta de alimentos contaminados pueden provocar enfermedades transmitidas por los alimentos (ETAs). La presencia de E. coli y Coliformes son indicadores de contaminación por una mala manipulación, en el mercado 9 de octubre de Cuenca, Ecuador se examinó la calidad microbiológica de ensaladas cocidas siendo uno de los principales alimentos que se encuentran listos para ser consumidos, previniendo la alteración del alimento y garantizando la seguridad alimentaria. Objetivo: Determinar la presencia de E. coli/Coliformes en ensaladas cocidas expendidas en el mercado 9 de octubre de la ciudad de Cuenca, periodo agosto 2023. Metodología: Para el desarrollo de este artículo se emplea un diseño de tipo experimental de análisis microbiológico de laboratorio. El universo de este estudio está conformado por el mercado 9 de octubre de la ciudad de Cuenca. Fueron analizadas 30 muestras de ensaladas cocidas para la determinación de E. coli/Coliformes, con el uso de placas Compact Dry EC. Se cuantificó la presencia de UFC según las normativas INEN 1529- 7 y NTS N° 071- MINSA/DIGESA/V 01. Resultados: Se identificó la presencia de E. coli/Coliformes por unidades formadoras de colonias UFC/g en las muestras, presentando una superación del límite permisible mencionado en la normativa peruana, determinando que es inaceptable el expendio y consumo de este alimento. Conclusión: Se analizó la calidad microbiológica de las ensaladas cocidas en el mercado 9 de octubre, con evidencia de riesgo sanitario, debido a un alto crecimiento de microorganismos observadas en las placas Compact Dry. EC. Área de estudio general: Microbiología. Área de estudio específica: Bacteriología. Tipo de estudio: Artículo original.
Clinical Manifestations and Neuroimaging Features of Intracranial Infection among Study at a Tertiary Care Hospital in Bangladesh
Authors: Khan Ashraf Uddin , Sajedeen Sharmeen , Akhter Niksar , Moureen Adneen , Sattar AFM Arshedi , Hassan Mohammad Sayeed 
Published: February 2025

Background: Intracranial infection is one of the life-threatening infections that can affect the meninges or the brain. It is a major cause of emergency and death in children and adults. Preconception regarding the clinical manifestation of intracranial infection may be helpful for physicians in managing such emergency cases.
Objective: This study was aimed to assess the clinical manifestation and neuro-radiological features among patients presented with intracranial infections.
Methodology: This was a cross-sectional observational study that was conducted in the Department of Radiology and Imaging (CT section) of BSMMU and Dhaka Medical College Hospital, Dhaka, Bangladesh from March 2006 to September 2006. In total 50 patients, by using the spiral CT system with various complaints suspecting intracranial infections and few number of pre-diagnose cases for evaluation of complications were enrolled in this study as the study subjects by random sampling technique. Data were processed, analyzed and disseminated by using MS Office.
Results: Male patients contributed 66.0% of the total; most of the patients who underwent a CT of the brain were from 31 to 50 years old. As the most frequent complaint, fever, headache and vomiting were observed in 24.0%, 20.0% and 16.0% of the cases; in about half of the patients (46.0%) high-grade fever was found. As the CT finding, abnormal meningeal enhancement (11.5%), cerebral oedema (7.5%) and mild ventricular dilatation with subarachnoid space enlargement (8.5%) were found among a noticeable number of patients.
Conclusion: The prevalence of intracranial infection among males is about double that in females. Fever, headache and vomiting are the most common complaints of intracranial infection patients. Abnormal meningeal enhancement, cerebral oedema and mild ventricular dilatation with subarachnoid space enlargement are very common CT findings in such cases.
Bangladesh Journal of Medical Microbiology, July 2024;18(2):75-79
Perceived bioethical issues in cancer rehabilitation: a qualitative study among Italian physiotherapists
Authors: Bertoni Gianluca , Battista Simone , Conti Valentina , Testa Marco , Patuzzo Manzati Sara 
Published: February 2025

Introduction: Literature on bioethics in physiotherapy, particularly in cancer management, is limited. This study explores the perceived bioethical issues in cancer rehabilitation by Italian physiotherapists.
Participants: Thirty-one physiotherapists (Age: 42 ± 10.5 years; 20 women, 11 men) with expertise in cancer rehabilitation were purposefully selected.
Data Collection: Six online focus groups were conducted, guided by a focus group guide based on existing literature and refined by experts in cancer rehabilitation and bioethics.
Data Analysis: Sessions were recorded, transcribed, and analyzed using Braun and Clarke’s ‘Reflexive Thematic Analysis’.
Results: Four primary themes emerged: 1) Challenges of (Non)-Disclosure in Diagnosis and Prognosis – ethical difficulties around withholding diagnosis or prognosis information; 2) Balancing Hope and Realism in Patient and Caregiver Expectations – navigating hope versus realistic rehabilitation goals; 3) Weighing Efficacy and Safety in Cancer Rehabilitation – balancing ­treatment outcomes with patient safety; 4) Decisions on Withdrawing Treatment – ethical considerations in discontinuing treatment.
Discussion: These themes highlight common ethical dilemmas faced by physiotherapists in cancer rehabilitation, mirroring broader healthcare challenges. Addressing them requires a nuanced understanding of ethical principles within the cancer rehabilitation context.
Conclusions: The study provides insights into the bioethical issues in cancer rehabilitation, stressing the need for a patient-centered approach to navigate these challenges effectively.
Improved blockchain-based ECDSA batch verification scheme
Authors: Wu Guangfu , Zhou Jiandong , Fu Xiaoyan 
Published: February 2025

IntroductionBlockchain technology has attracted much attention due to its decentralization, transparency and security. Initially applied in the financial field, it has now expanded to various fields such as Internet of Things (IoT), electronic cash and healthcare. However, the open nature of blockchain has raised potential security concerns about sensitive transaction data, and the increasing number of transactions requires low-latency solutions. Most blockchain applications still rely on the lightweight Elliptic Curve Digital Signature Algorithm (ECDSA). Due to complex operations such as vectorized multiplication and modular inversion, this may introduce significant additional overhead.MethodsTo address these issues, a new scheme named KTP-ECDSA is proposed. This scheme is based on the improved two-parameter Elliptic Curve Digital Signature Algorithm (TP-ECDSA) and the KGLP algorithm. In both the signing and verification processes, this scheme eliminates modular inverse operations and reduces scalar multiplications during the verification stage by using batch verification.ResultThe experimental results show that, compared with the traditional ECDSA, KTP-ECDSA has achieved a speed increase of over 50% in both independent verification and batch verification, significantly improving the efficiency of signature verification.DiscussionBy adopting the KTP-ECDSA algorithm and using the digital signature batch verification method, multiple signatures can be verified simultaneously, thus reducing the computational burden of the traditional single-verification method. This greatly increases the overall transaction throughput and improves resource utilization efficiency.
Single-cell and spatial analysis reveals the interaction between ITLN1+ foam cells and SPP1+ macrophages in atherosclerosis
Authors: Li Ying , Wang Shanshan , Zhang Ruidan , Gong Yingying , Che Yulu , Li Kening , Pan Zongfu 
Published: February 2025

IntroductionCardiovascular disease (CVD) caused by atherosclerosis (AS) remains the leading cause of mortality in developed countries. Understanding cellular heterogeneity within the inflammatory microenvironment is crucial for advancing disease management strategies. This study investigates the regulatory functions of distinct cell populations in AS pathogenesis, focusing on the interaction between vascular smooth muscle cell (VSMC)-derived ITLN1+ foam cells and SPP1+ FABP5+ macrophages.MethodsWe employed single-cell RNA sequencing to characterize cell populations within AS plaques. Correlation analyses and the CellChat package were utilized to elucidate intercellular communication networks among various cell types. The functional roles of key subsets of macrophages and VSMCs were assessed using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. Pseudotime trajectory analysis was conducted to explore the dynamics of VSMC differentiation. Additionally, spatial transcriptomics analysis was used to demonstrate the physical interactions between different cell subpopulations.ResultsWe identified significant infiltration of macrophage clusters in AS, with SPP1+ FABP5+ macrophages being highly enriched in AS plaques. These macrophages were associated with lipid transport, storage, and cell migration pathways. A distinct subset of ITLN1+ foam cells derived from VSMCs exhibited robust expression of foam cell markers and lipid metabolism-related genes. Pseudotime trajectory analysis indicated that ITLN1+ foam cells represent a terminal stage of VSMC differentiation, characterized by elevated expression of genes linked to lipid synthesis and AS progression. Spatial transcriptomics and CellChat analysis revealed a significant interaction between ITLN1+ foam cells and SPP1+ FABP5+ macrophages, mediated by the MIF-(CD74 + CD44) and SPP1-CD44 ligand-receptor axes.DiscussionOur findings underscore the critical crosstalk between ITLN1+ foam cells and SPP1+ macrophages in promoting lipid accumulation and AS progression. Targeting this cell-cell interaction may offer new therapeutic avenues for managing atherosclerosis. Further validation of these mechanisms is necessary to develop effective immunotherapeutic strategies against AS.
Effects of sodium tanshinone IIA sulfonate injection on pro-inflammatory cytokines, adhesion molecules and chemokines in Chinese patients with atherosclerosis and atherosclerotic cardiovascular disease: a meta-analysis of randomized controlled trials
Authors: Xie Fan , Fu Xiaoying , Li Wenbo , Bao Yujin , Chang Feng , Lu Yun , Lu Yuqiong 
Published: February 2025

BackgroundInflammation, as the basic pathogenic mechanism of atherosclerosis, promotes the development of atherosclerosis (AS) and atherosclerotic cardiovascular disease (ASCVD). In numerous experiments based on animal and cellular models, sodium tanshinone IIA sulfonate (STS) injection has been found to reduce the levels of pro-inflammatory cytokines, adhesion molecules, and chemokines in patients with AS and ASCVD, exerting an anti-inflammatory effect to treat the disease.ObjectivesThis study aimed to perform a meta-analysis of randomized controlled trials (RCTs) to quantify the effects of STS on pro-inflammatory cytokines, adhesion molecules, and chemokines in patients with AS and ASCVD.MethodsEight literature databases were searched from inception to January 2024, including PubMed, Web of Science, Cochrane Library, Ebsco, CNKI, VIP, WanFang Data, and ClinicalTrails.gov. Two reviewers independently screened articles and extracted data. The quality of the included studies was assessed using the Cochrane Risk Assessment Tool 2.0. Meta-analysis was performed using RevMan 5.4 software.ResultsOf the 2,698 publications screened, 42 studies were included, and the related trials involved 4,654 Chinese patients. The meta-analysis showed that STS significantly reduced the concentration level of pro-inflammatory cytokines interleukin 6 (IL-6) [standardized mean difference (SMD)=−1.50, 95%CI(−2.06, −0.95), p &lt; 0.00001], tumor necrosis factor-α (TNF-α) [SMD = −2.55, 95%CI(−3.24, −1.86), p &lt; 0.00001], and interleukin-1β (IL-1β) [SMD = −1.21, 95%CI(−2.41, −0.01), p &lt; 0.0001], of adhesion molecules intercellular adhesion molecule-1 (ICAM-1) [SMD = −1.28, 95%CI(−1.55, −1.02), p &lt; 0.00001] and p-selectin [SMD = −1.06, 95%CI(−1.46, −0.67), p &lt; 0.00001], and of chemokines fractalkine [SMD = −1.32, 95%CI(−2.02, −0.61), p = 0.0003] and monocyte chemoattractant protein-1 (MCP-1) [SMD = −0.83, 95%CI(−1.11, −0.55), p &lt; 0.00001] among patients with AS and ASCVD.ConclusionThe use of STS in patients with AS and ASCVD appeared to significantly decrease levels of pro-inflammatory cytokines, adhesion molecules, and chemokines.Systematic Review Registration: [https://www.crd.york.ac.uk/PROSPERO/], PROSPERO [CRD42024496960].
Effect of environmental stress factors on the expression of virulence genes and pathogenicity of lethal Bacillus cereus of bovine origin
Authors: Meng Qinglei , Song Lili , Chi Shanshan , Wang Haifeng , Li Jie , Chen Yunjiao , Liu Zhilin , Zhang Xin , Jia Zelin , Cui Jiayu , Wang Xueli 
Published: February 2025

ObjectiveBacillus cereus (B. cereus) can be used as a probiotic or produce a variety of toxins that are pathogenic to humans and animals. Environmental stressors can affect the growth process of B. cereus and the expression of its virulence genes. Due to the limitations of methods such as pharmacological disinfection methods (there are limits to the use of antibiotics) and chemical disinfection methods (chemical methods may produce residues), attempts can be made to remove and reduce B. cereus infections through environmental stress factors.MethodsIn this study, the expression of four virulence genes (nheA, hblD, cytK, and entFM) of bovine-origin lethal B. cereus was investigated by qPCR under the effect of different environmental stressors. The extent of pathological damage to various organs of mice by B. cereus was observed by pathological sections.ResultsThe results showed that high temperature could inhibit the expression of B. cereus virulence genes. Expression of B. cereus virulence genes was affected under the influence of pH. Different salt concentrations could make the B. cereus virulence genes show low expression. Under a single environmental stressors, nheA, hblD, cytK, and entFM had the lowest expression at 40°C, pH 8.0, and were lowly expressed at all salt concentrations except the control group. The action of multiple environmental stressors affect the expression of virulence genes. Under multiple environmental stressors, nheA, hblD and cytK were least expressed at a temperature of 40°C, pH 6.0, and salt concentration of 3.0%, and entFM was least expressed at a temperature of 20°C, pH 8.0, and salt concentration of 1.5%. Animal pathogenicity tests have shown that environmental stressors affect the virulence of B. cereus.ConclusionThe level of virulence gene expression in B. cereus can be reduced by environmental stress factors, thus further reducing the risk of B. cereus to human health. This study provides some reference for the prevention and control of B. cereus disease.
UPLC-MS/MS method for simultaneous determination of pyrantel, praziquantel, febantel, fenbendazole and oxfendazole in dog plasma and its application to a bioequivalence study
Authors: Zhang Fengyichi , Guo Fanxi , Zhao Xinyu , Li Chongyang , Wang Junli , Wang Mengyun , Ma Ruixia , Chen Baochang , Miao Qiangqiang , Wang Yimeng , Wang Zihan , Cao Di , Yu Zugong 
Published: February 2025

A rapid, sensitive and reliable ultra–performance liquid chromatography–tandem mass spectrometry (UPLC–MS/MS) was developed and validated for the simultaneous quantitation of pyrantel (PYR), praziquantel (PRA), febantel (FBT) and its active metabolites, fenbendazole (FEN) and oxfendazole (OXF) in dog plasma. Fenbendazole–D3 (FEN–D3) was used as an internal standard (IS). The analytes and IS were prepared using simple protein precipitation (PP) combined with liquid–liquid extraction (LLE). Chromatographic analysis was performed on UPLC BEH C18 column using acetonitrile–0.1% formic acid in water for gradient elution. Detection was carried out in multiple reaction monitoring (MRM) mode under positive electrospray ionization. The standard curves were linear through the concentration range of 4–240 ng/mL for PYR and OXF, 15–900 ng/mL for PRA, 2–120 ng/mL for FBT and 10–600 ng/mL for FEN with all correlation coefficients &gt;0.99. The intra–and inter–batch precision was 1.08%–14.26% and accuracy was from 90.66% to 110.28%. The mean extraction recoveries for the analytes and IS were &gt;90%. The total run time was 9.0 min. The developed method was successfully applied to a bioequivalence study after oral administration of compound febantel tablets in 38 healthy dogs.
Transcranial magnetic stimulation in attention-deficit/hyperactivity disorder: a systematic review and meta-analysis of cortical excitability and therapeutic efficacy
Authors: Han Yu , Wei Zi-Yu , Zhao Na , Zhuang Qian , Zhang Hang , Fang Hong-Li , Zang Yu-Feng , Feng Zi-Jian 
Published: February 2025

Background/ObjectivesAttention-deficit/hyperactivity disorder (ADHD) currently lacks a universally accepted biomarker or diagnostic test, underscoring the need for objective and effective assessment methods. Transcranial magnetic stimulation (TMS) has emerged as a promising tool for both assessing cortical excitability and providing therapeutic interventions. This study conducted two independent meta-analyses to evaluate: 1) the potential of TMS in assessing cortical excitability, and 2) its therapeutic efficacy in managing ADHD symptoms.MethodsA systematic search was conducted in EMBASE, MEDLINE, PsycINFO, ClinicalTrials, and PubMed following PRISMA guidelines. The “cortical excitability” meta-analysis included studies comparing TMS-EMG or TMS-EEG neurophysiological measures between ADHD patients and healthy controls. The “therapeutic“ meta-analysis focused on randomized controlled trials (RCTs) evaluating repetitive TMS (rTMS) effects on ADHD symptoms. Standardized mean differences (SMDs) were calculated for pooled effect sizes.ResultsIn the “cortical excitability” meta-analysis, 17 studies were included, demonstrating significantly reduced short-interval intracortical inhibition (SICI) in ADHD compared to healthy controls (pooled SMD = 0.65, 95% CI: 0.41–0.88, P &lt; 0.00001). No significant differences were observed for motor evoked potentials (MEP), motor thresholds (aMT/rMT), cortical silent period (cSP), ipsilateral silent period (iSP), or intracortical facilitation (ICF). The “therapeutic“ meta-analysis, encompassing 8 samples from 7 studies, demonstrated that rTMS significantly improved ADHD symptoms compared to control conditions (pooled SMD = 0.45, 95% CI: 0.19–0.70, P = 0.0006).ConclusionsThis study highlights the potential of TMS as both a diagnostic and therapeutic tool in ADHD. Reduced SICI appears to be a key neurophysiological marker of ADHD, reflecting cortical GABAergic dysfunction. Additionally, rTMS shows promise in alleviating ADHD symptoms, though further studies are needed to confirm long-term therapeutic benefits and optimize stimulation protocols.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, identifier CRD42024507867.
The Impact Assessment of the Mineral Aggregates’ Deposits on the Storage Area
Authors: ȘERBAN Anca , LEOPA Adrian 
Published: February 2025

The construction sector is vital for our modern society such that most of the modern activities are dependent on constructions. The demographic increase and the longing of our times to better living conditions are the main exponents of unprecedented development in the construction field. The consequence of this phenomenon is the continuous growth of waste from construction and demolition. The management of these waste according to the sustainable development principles involves recycling or where not possible their storage considering the environmental tasks. This paper marks out the management of the two construction and demolition wastes, gas-formed concrete and concrete, taking account of the chemical composition of the leachate resulted from the waste drainage in the ground due to precipitation.
Prediction of Deformation of Hexagonal Honeycomb Blast Structure Under Explosive Loading Using Deep Learning
Authors: MARIN Mihaela , MARIN Florin-Bogdan 
Published: February 2025

Honeycomb composites are widely used in blast structure under explosive loading because of mechanical properties. The simulation of high-pressure explosion is time consuming in order to simulate an important number of scenarios. New deep learning neural models might approximate results with low computational resources outputting the result very fast. The purpose of this study is to propose using deep learning model using a relative low amount of training fata to approximate deformation in honeycomb structures subjected to a blast load. This study employed variation of hexagonal honeycomb dimensions to determine the deformation using deep learning model.
Experimental Research on the Influence of Lens Cleaning Products, on Their Hardness
Authors: TUDOR Beatrice Daniela , BĂBUȘANU Iustina 
Published: February 2025

The paper presents a study on the influence of optical lens cleaning products for vision correction on their hardness. The lenses under study were previously cleaned repeatedly with different cleaning products, namely, special eyeglass wipes and liquid detergent. The research was carried out on a group of lenses with and without protection, of different thicknesses and diopters.
Chemical Water Pollutants in Ganga Water at Digha Ghat in Patna
Authors: - SANTOSH KUMAR 
Published: February 2025

I.	Abstract
This research investigates the presence and concentration of chemical water pollutants in the Ganga River at Digha Ghat in Patna, addressing the critical issue of water quality deterioration and its potential impacts on public health and local ecosystems. Through systematic water sampling and analysis, this study quantified levels of heavy metals, nutrients, and organic compounds, revealing significant concentrations of pollutants that exceed permissible limits established by health authorities. Notably, elevated levels of arsenic, lead, and nitrates were detected, which pose substantial risks to human health, including chronic diseases and developmental disorders. The findings underscore the urgent need for effective water management strategies to mitigate pollution and safeguard public health, particularly for communities relying on the Ganga for drinking and agricultural purposes. Furthermore, this research contributes to the broader understanding of environmental health by highlighting the interconnections between water quality, ecological health, and community well-being. The implications extend to policy-making and health care interventions, fostering a proactive approach to water pollution control that emphasizes community awareness and sustainable practices. Ultimately, the study serves as a critical call to action for both local authorities and communities, aiming to enhance the health outcomes and ecological integrity of the Ganga River system.