bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transgenerational effects of infantile adversity and enrichment in male and female rats
Authors: Leshem Micah , Schulkin Jay 
Published: August 2011

AbstractTo discover whether the accumulation of life's experiences, adverse and enriching, inform, and serve the following generation by inheritance we examine whether stress to a weanling female will influence her future offspring, whether prereproductive enrichment to the dam, or postweaning enrichment to the offspring, can reverse the transgenerational effects of stress, and whether, like adversity, enrichment might have transgenerational effects. Female rats were exposed to stressors when they were 27–29 days old. Half of these females and their controls were then raised in an enriched environment from weaning until mating at 60 days to examine whether preproduction enrichment reverses the effects of preproduction stress on offspring. Half of the offspring of each group were raised in an enriched environment after weaning, to see whether it reverses the effects of preproduction stress and buttresses prereproductive enrichment. Behavior was examined in 625 adult offspring in 16 groups covering all permutations of the experimental variables (preproduction weanling stress (PS), preproduction enrichment (PE), offspring enrichment (OE), sex). Exploration, avoidance learning, startle, and social interaction were tested. Results reveal that very early prereproductive experience in females, adverse or enriching, will transgenerationally influence their future offspring, depending on the behavior tested and sex. Our finding that enrichment, whether to the parent or offspring generation, can ameliorate the transgenerational impact of adversity, has novel implications for the malleability of transgenerational inheritance, and its individual, social, and therapeutic impact. © 2011 Wiley Periodicals, Inc. Dev Psychobiol 54:169‐186, 2012.
Phylogeography of the antMyrmica rubraand its inquiline social parasite
Authors: Leppänen Jenni , Vepsäläinen Kari , Savolainen Riitta 
Published: August 2011

AbstractWidely distributed Palearctic insects are ideal to study phylogeographic patterns owing to their high potential to survive in many Pleistocene refugia and—after the glaciation—to recolonize vast, continuous areas. Nevertheless, such species have received little phylogeographic attention. Here, we investigated the Pleistocene refugia and subsequent postglacial colonization of the common, abundant, and widely distributed antMyrmica rubraover most of its Palearctic area, using mitochondrial DNA (mtDNA). The western and eastern populations ofM. rubrabelonged predominantly to separate haplogroups, which formed a broad secondary contact zone in Central Europe. The distribution of genetic diversity and haplogroups implied thatM. rubrasurvived the last glaciation in multiple refugia located over an extensive area from Iberia in the west to Siberia in the east, and colonized its present areas of distribution along several routes. The matrilineal genetic structure ofM. rubrawas probably formed during the last glaciation and subsequent postglacial expansion. Additionally, becauseM. rubrahas two queen morphs, the obligately socially parasitic microgyne and its macrogyne host, we tested the suggested speciation of the parasite. Locally, the parasite and host usually belonged to the same haplogroup but differed in haplotype frequencies. This indicates that genetic differentiation between the morphs is a universal pattern and thus incipient, sympatric speciation of the parasite from its host is possible. If speciation is taking place, however, it is not yet visible as lineage sorting of the mtDNA between the morphs.
DMF‐Catalyzed Direct and Regioselective C–H Functionalization: Electrophilic/Nucleophilic 4‐Halogenation of 3‐Oxypyr­azoles
Authors: Liu Yuanyuan , He Guangke , Chen Kai , Jin Yin , Li Yufeng , Zhu Hongjun 
Published: August 2011

AbstractA novel, straightforward, and highly regioselective 4‐chlorination of 3‐oxypyrazole derivatives in boiling thionyl chloride (SOCl2) in the presence of catalytic N,N‐dimethylformamide (DMF) has been developed. This reaction likely proceeds through a DMF‐catalyzed electrophilic/nucleophilic chlorination mechanism and involves electrophilic attack by SOCl2 and nucleophilic substitution by Cl– as the key steps. Based on this mechanism, the corresponding 4‐bromination and 4‐iodination of 3‐oxypyrazoles have also been achieved in good yield by adding Br– and I–, respectively, to the reaction system. This halogenation method enables quick access to many original 4‐halo‐3‐oxypyrazole series.
Ar‐BINMOLs with Axial and sp3Central Chirality – Characterization, Chiroptical Properties, and Application in Asymmetric Catalysis
Authors: Gao Guang , Bai Xing‐Feng , Yang Hua‐Meng , Jiang Jian‐Xiong , Lai Guo‐Qiao , Xu Li‐Wen 
Published: August 2011

Abstract2′‐Hydroxy‐1,1′‐binaphthalene‐2‐(arylmethanol) (Ar‐BINMOLs), a family of new 1,1′‐binaphthalene‐2,2′‐diol derivatives, prepared by a [1,2]‐Wittig rearrangement, are introduced as new chiral molecules for the study of the modes of supramolecular aggregation on the basis of ESI‐MS, NMR spectroscopy, differential scanning calorimetry (DSC), and X‐ray diffractometry. Characterization showed that Ar‐BINMOLs exhibit different characteristics to BINOL and act as supramolecules due to hydrogen bonding and aromatic π–π/C–H···π interactions. In particular, CD measurements of chiral and racemic Ar‐BINMOL2ain solution and the solid state show a strong Cotton effect, which revealed that amplification of chirality was observed because of the formation of a chiral supramolecular oligomer derived from the racemic monomer. To this end, the Ar‐BINMOLs were used as supramolecular auxiliaries to mediate the Michael reaction of anthrone to (E)‐β‐nitrostyrene and could give the Michael adduct with comparable enantioselectivities. In addition, the Ar‐BINMOLs act as ligands to promote asymmetric 1,2‐addition of diethylzinc to aldehydes, which resulted in excellent yields and enantioselectivities (up to >99.9 %ee) in the reactions of diethylzinc with a broad range of aromatic aldehdyes.
Electrochemical Performance of a Ni and YSZ Composite Synthesised by Ultrasonic Spray Pyrolysis as an Anode for SOFCs
Authors: Park H. J. , Shao Z. , Wang W. , Moon K. S. 
Published: August 2011

AbstractThe electrochemical performance of an anode material for a solid oxide fuel cell (SOFC) depends highly on microstructure in addition to composition. In this study, a NiO–yttria‐stabilised zirconia (NiO–YSZ) composite with a highly dispersed microstructure and large pore volume/surface area has been synthesised by ultrasonic spray pyrolysis (USP) and its electrochemical characteristics has been investigated. For comparison, the electrochemical performance of a conventional NiO–YSZ is also evaluated. The power density of the zirconia electrolyte‐supported SOFC with the synthesised anode is ∼392 mW cm–2 at 900 °C and that of the SOFC with the conventional NiO–YSZ anode is ∼315 mW cm–2. The improvement is ∼24%. This result demonstrates that the synthesised NiO–YSZ is a potential alternative anode material for SOFCs fabricated with a zirconia solid electrolyte.
Extracellular ATP induces CD44 shedding from macrophage‐like P388D1 cells via the P2X7 receptor
Authors: Lin Changwei , Ren Shuangyi , Zhang Li , Jin Hongfei , Sun Jianguo , Zuo Yunfei 
Published: August 2011

AbstractThe P2X7 receptor (P2X7R) is a nucleotide receptor expressed predominantly on hemopoietic, bone, and epithelial cells. The P2X7R can be activated by extracellular ATP and induces the influx of calcium, releases cytokines, and participates in cell proliferation and apoptosis. CD44 is an adhesion molecule. The effects of CD44 include cell–cell and cell–matrix adhesion interactions, lymphocyte activation, and cell migration. Many studies have shown that P2X7R and CD44 play important roles in hematological malignancies, but no study exists regarding the relationship between P2X7R and CD44. In the present study, we characterized P388D1 cells for the surface expression of CD44 and analyzed ATP‐induced shedding. The data showed that P388D1 cells express CD44. Incubation of P388D1 cells with ATP induced a rapid loss of CD44 from the P388D1 cell surface. In addition, using a receptor inhibitor and P2X7R short hairpin RNA, we showed that the loss of CD44 is mediated via the P2X7R. Finally, we demonstrated that activation of P2X7R by ATP induces CD44 shedding. Copyright © 2011 John Wiley & Sons, Ltd.
Immunophenotype distinction between acute promyelocytic leukaemia and CD15− CD34− HLA‐DR− acute myeloid leukaemia with nucleophosmin mutations
Authors: Ferrari Angela , Bussaglia Elena , Úbeda Josep , Facchini Luca , Aventin Anna , Sierra Jorge , Nomdedéu Josep F. 
Published: August 2011

AbstractAcute promyelocytic leukaemia (APL) is a unique clinicobiologic entity that can be successfully treated with All‐trans Retinoic Acid ATRA‐based regimens. Some cases of acute myeloid leukaemia (AML) with nucleophosmin (NPM) mutations have an immunophenotype that is similar to APL. The objective of the study is to compare antigenic expression in a group of APL patients with that in AML patients with NPM mutations and an APL‐like immunophenotype (CD15− CD34− HLA‐DR−). A consecutive series of 40 APL and 12 NPM patients with an APL‐like phenotype were included in the study. Immunophenotypic patterns were investigated by multiparametric flow cytometry. Promyelocytic leukaemia–retinoic acid receptor‐α transcript type, NPM and FLT3 mutations were investigated using conventional methods. Statistically significant differences were found between APL and NPM‐mutated AML in CD33, CD13, CD2 and CD110 reactivity. CD2 expression was absent in every patient with NPM‐mutated AML. In addition, mean fluorescence intensity and the coefficient of variation (cv) of CD33 and CD13 showed statistical differences between the two groups for CD33 (p = 0.007) and a trend to significance for CD13 (p = 0.05). Furthermore, among 45 evaluable patients, CD110 expression statistically differentiates between the two groups: [2/33 (6%) in the APL group and 8/12 (66.6%) in the NPM‐mutated AML (p = 0.014)]. However, these traits were subtle, raising the possibility of practical diagnostic challenges. In conclusion, CD110 and CD33 reactivity may be useful to distinguish APL from NPM‐mutated AML with CD15, CD34 and HLA‐DR negativity. Nevertheless, cytogenetic and molecular characterization is necessary to establish the accurate diagnosis of AML. Copyright © 2011 John Wiley & Sons, Ltd.
High prevalence and breast cancer predisposing role of the BLM c.1642 C>T (Q548X) mutation in Russia
Authors: Sokolenko Anna P. , Iyevleva Aglaya G. , Preobrazhenskaya Elena V. , Mitiushkina Nathalia V. , Abysheva Svetlana N. , Suspitsin Evgeny N. , Kuligina Ekatherina Sh. , Gorodnova Tatiana V. , Pfeifer Werner , Togo Alexandr V. , Turkevich Elena A. , Ivantsov Alexandr O. , Voskresenskiy Dmitry V. , Dolmatov Georgy D. , Bit‐Sava Elena M. , Matsko Dmitry E. , Semiglazov Vladimir F. , Fichtner Iduna , Larionov Alexey A. , Kuznetsov Sergey G. , Antoniou Antonis C. , Imyanitov Evgeny N. 
Published: August 2011

AbstractThe BLM gene belongs to the RecQ helicase family and has been implicated in the maintenance of genomic stability. Its homozygous germline inactivation causes Bloom syndrome, a severe genetic disorder characterized by growth retardation, impaired fertility and highly elevated cancer risk. We hypothesized that BLM is a candidate gene for breast cancer (BC) predisposition. Sequencing of its entire coding region in 95 genetically enriched Russian BC patients identified two heterozygous carriers of the c.1642 C>T (Q548X) mutation. The extended study revealed this allele in 17/1,498 (1.1%) BC cases vs. 2/1,093 (0.2%) healthy women (p = 0.004). There was a suggestion that BLM mutations were more common in patients reporting first‐degree family history of BC (6/251 (2.4%) vs. 11/1,247 (0.9%), p = 0.05), early‐onset cases (12/762 (1.6%) vs. 5/736 (0.7%), p = 0.14) and women with bilateral appearance of the disease (2/122 (1.6%) vs. 15/1376 (1.1%), p = 0.64). None of the BLM‐associated BC exhibited somatic loss of heterozygosity at the BLM gene locus. This study demonstrates that BLM Q548X allele is recurrent in Slavic subjects and may be associated with BC risk.
BARD1: An independent predictor of survival in non‐small cell lung cancer
Authors: Zhang Yong‐Qiang , Bianco Andrea , Malkinson Alvin M. , Leoni Vera Piera , Frau Gianni , De Rosa Nicolina , André Pierre‐Alain , Versace Renato , Boulvain Michel , Laurent Geoffrey J. , Atzori Luigi , Irminger‐Finger Irmgard 
Published: August 2011

AbstractBRCA1 mRNA overexpression is correlated with poor survival in NSCLC. However, BRCA1 functions depend on the interaction with BARD1 for its stability, nuclear localization and ubiquitin ligase activity. Expression of alternatively spliced BARD1 isoforms that lack the BRCA1‐interaction domain was found upregulated and correlated with poor prognosis in breast and ovarian cancer. These BARD1 isoforms are essential for proliferation of cancer cells in vitro. We investigated whether BARD1 isoforms are expressed in NSCLC. While in lung tissues from healthy controls BARD1 expression was undetectable on the mRNA level and protein level, we found two novel isoforms in addition to previously identified mRNAs expressed in all NSCLC samples tested. Furthermore, the pattern of BARD1 isoform expression was similar in tumor and morphologically normal peri‐tumor tissues, and only one novel isoform π was specifically upregulated in tumors. Immunohistochemistry revealed that all 100 NSCLC cases tested expressed isoform‐specific BARD1 epitopes, while BARD1 expression was undetectable in biopsies from healthy controls. Statistical analysis showed that the expression of epitopes PVC and WFS, present on isoform π, or epitope WFS alone, expressed on isoforms π, κ and β, were significantly correlated with decreased patient survival. These findings were corroborated in a mouse model of chemically induced lung cancer. Immunostaining of mouse tumors showed that BARD1 epitopes PVC and WFS were specifically upregulated in invasive, but not in confined lung tumors. Thus, BARD1 isoforms might be involved in tumor initiation and invasive progression and might represent a novel prognostic marker for NSCLC.
Mechanisms involved in α1B‐adrenoceptor desensitization
Authors: García‐Sáinz, J. Adolfo , Romero‐Ávila María Teresa , Alcántara‐Hernández Rocío 
Published: August 2011

Abstractα1B‐Adrenergic receptors mediate many of the actions of the natural catecholamines, adrenaline and noradrenaline. They belong to the seven transmembrane domains G protein‐coupled receptor superfamily and exert their actions mainly through activation of Gq proteins and phosphoinositide turnover/calcium signaling. Many hormones and neurotransmitters are capable of inducing α1B‐adrenergic receptor phosphorylation and desensitization; among them: adrenaline and noradrenaline, phorbol esters, endothelin‐I, bradykinin, lysophosphatidic acid, insulin, EGF, PDGF, IGF‐I, TGF‐β, and estrogens. Key protein kinases for these effects are G protein coupled receptor kinases and protein kinase C. The lipid/protein kinase, phosphoinositide‐3 kinase also appears to play a key role, acting upstream of protein kinase C. In addition to the agents employed for cells stimulation, we observed that paracrine/autocrine mediators also participate; these processes include EGF transactivation and sphingosine‐1‐phosphate production and action. The complex regulation of these receptors unlocks opportunities for therapeutic intervention. © 2011 IUBMB IUBMB Life, 2011
Glycan reader: Automated sugar identification and simulation preparation for carbohydrates and glycoproteins
Authors: Jo Sunhwan , Song Kevin C. , Desaire Heather , MacKerell Alexander D. , Im Wonpil 
Published: August 2011

AbstractUnderstanding how glycosylation affects protein structure, dynamics, and function is an emerging and challenging problem in biology. As a first step toward glycan modeling in the context of structural glycobiology, we have developed Glycan Reader and integrated it into the CHARMM‐GUI, http://www.charmm‐gui.org/input/glycan. Glycan Reader greatly simplifies the reading of PDB structure files containing glycans through (i) detection of carbohydrate molecules, (ii) automatic annotation of carbohydrates based on their three‐dimensional structures, (iii) recognition of glycosidic linkages between carbohydrates as well as N‐/O‐glycosidic linkages to proteins, and (iv) generation of inputs for the biomolecular simulation program CHARMM with the proper glycosidic linkage setup. In addition, Glycan Reader is linked to other functional modules in CHARMM‐GUI, allowing users to easily generate carbohydrate or glycoprotein molecular simulation systems in solution or membrane environments and visualize the electrostatic potential on glycoprotein surfaces. These tools are useful for studying the impact of glycosylation on protein structure and dynamics. © 2011 Wiley Periodicals, Inc. J Comput Chem, 2011
Toward a new approach for determination of solute's charge distribution to analyze interatomic electrostatic interactions in quantum mechanical/molecular mechanical simulations
Authors: Yamada Kenta , Koyano Yoshiyuki , Okamoto Takuya , Asada Toshio , Koga Nobuaki , Nagaoka Masataka 
Published: August 2011

AbstractWe present an alternative approach to determine “density‐dependent property”‐derived charges for molecules in the condensed phase. In the case of a solution, it is essential to take into consideration the electron polarization of molecules in the active site of this system. The solute and solvent molecules in this site have to be described by a quantum mechanical technique and the others are allowed to be treated by a molecular mechanical method (QM/MM scheme). For calculations based on this scheme, using the forces and interaction energy as density‐dependent property our charges from interaction energy and forces (CHIEF) approach can provide the atom‐centered charges on the solute atoms. These charges reproduce well the electrostatic potentials around the solvent molecules and present properly the picture of the electron density of the QM subsystem in the solution system. Thus, the CHIEF charges can be considered as the atomic charges under the conditions of the QM/MM simulation, and then enable one to analyze electrostatic interactions between atoms in the QM and MM regions. This approach would give a view of the QM nuclei and electrons different from the conventional methods. © 2011 Wiley Periodicals, Inc. J Comput Chem 2011
First‐principles prediction on electronic and magnetic properties of hydrogenated AlN nanosheets
Authors: Zhang Chang‐wen , Zheng Fu‐bao 
Published: August 2011

AbstractBased on first‐principles calculations, the geometric, electronic, and magnetic properties as well as the relative stability of the fully hydrogenated and semihydrogenated AlN nanosheets (NSs) have been investigated. The results show that different with the bare graphite‐like AlN NSs terminating with polar (0001) surfaces, the hydrogenated configurations preserve the initial wurtzite structure. Depending on surface hydrogenation and the thickness of AlN NSs, three magnetic configurations, that is, semiconductor, half metal, and metal states, are all observed. Analysis of formation energies indicates that, for the configuration n = 1, the hydrogen atoms adsorb on the top of Al sites is the most stable structure, while for the other configurations (n > 2), AlN‐nH structure is more favorable energetically. The results indicated that hydrogenation on different Al and N sites might be an efficient route to tune their electronic and magnetic properties to realize potential applications in the fields of electronics and spintronics. © 2011 Wiley Periodicals, Inc. J Comput Chem, 2011
Synthesis of novel 1,7‐naphthyridines by Friedländer condensation of pyridine substrates
Authors: Stockmann Vegar , Fiksdahl Anne 
Published: August 2011

AbstractThe general ability of appropriate pyridyl compounds (aldehyde or ketone) to undergo Friedländer condensation to give different 1,7‐naphthyridines has been demonstrated. 2,4‐Disubstituted 1,7‐naphthyridine 8 was prepared from 3‐amino‐4‐acetylpyridine (6) and ketone 4 (82%). The Friedländer self‐condensation of pyridyl substrate 6 is reported, as well. The dimer product, 2‐(3‐aminopyridin‐4‐yl)‐4‐methyl‐1,7‐naphthyridine (7), was obtained in 97% yield. 2‐Aryl‐ and 2,3‐diaryl‐1,7‐naphthyridines (16, 17, 18) were prepared from 3‐aminoisonicotinaldehyde (13) and arylketones 4, 14, and 15 (28–71%). The key substrates 6 and 13 are readily available via the improved pyridine nitration method. J. Heterocyclic Chem., (2011).
An efficient and convenient approach for the synthesis of novel 2‐hydroxy‐12‐aryl‐8,9,10,12‐tetrahydrobenzo[a]xanthene‐11‐ones using p‐toluenesulfonic acid in ethanol and ionic liquid
Authors: Khurana Jitender M. , Nand Bhaskara , Sneha  
Published: August 2011

AbstractA novel series of 2‐hydroxy‐12‐aryl‐8,9,10,12‐tetrahydrobenzo[a]xanthene‐11‐ones were synthesized by the one‐pot multicomponent condensation of 2,7‐dihydroxynaphthalene, aromatic aldehydes, and cyclic 1,3‐dicarbonyl compounds in the presence of a catalytic amount of p‐toluenesulfonic acid in ethanol and in ionic liquid butyl methyl imidazolium tetrafluroborate ([bmim]BF4). The newly developed protocol is operationally convenient, widely applicable, and gives excellent yields of the diversely substituted title compounds in high purity by easy workup. J. Heterocyclic Chem., (2011).
Noncovalent complexation of glucosylthioureidocalix[4]arenes with carboxylates and their gas‐phase characteristics: an ESI‐FTICR mass spectrometric study
Authors: Torvinen Mika , Kalenius Elina , Sansone Francesco , Casnati Alessandro , Jänis Janne 
Published: August 2011

AbstractThe noncovalent complexation of three glucosylcalix[4]arenes (1–3) towards 23 mono‐ and dicarboxylic acid anions were studied by ESI‐FTICR mass spectrometry. Competitive complexation, collision‐induced dissociation and gas‐phase H/D‐exchange experiments were performed to obtain information on selectivity of calixarenes towards carboxylates and characteristics of their complexes. The flexibility and number of glucose units of the host and the spatial disposition of the hydrogen bonding groups on the carboxylate guests were found to affect the selectivity of complexation strongly. The glucosylcalixarenes exhibited particular selectivity for dicarboxylic acid anions incorporating π‐systems, and clear isomeric selectivity was observed for isophthalic among phthalic acid anions and for fumaric acid over maleic acid anion. Copyright © 2011 John Wiley & Sons, Ltd.