bluuebunny/crossref_metadata_2025_split huggingface dataset:
The importance of Foxp3 antibody and fixation/permeabilization buffer combinations in identifying CD4+CD25+Foxp3+ regulatory T cells
Authors: Law Jacqueline P. , Hirschkorn Dale F. , Owen Rachel E. , Biswas Hope H. , Norris Philip J. , Lanteri Marion C. 
Published: October 2009

AbstractFoxp3 is a key marker for CD4+ regulatory T cells (Tregs) and was used in developing a multiparameter flow cytometric panel to identify Tregs. Achieving reproducible staining and analysis first required optimization of Foxp3 staining. We present a comparative study of PCH101, 236A/E7, 3G3, 206D, 150D, and 259D/C7 clones of anti‐human‐Foxp3 antibodies used in combination with five different fixation/permeabilization buffers. Staining for CD25, CD152, and CD127 was also compared between fixation/permeabilization treatments. Promising antibody/buffer combinations were tested in a panel of peripheral blood mononuclear cells from 10 individuals, and then on fresh versus frozen cells from four individuals. Finally, different fluorochromes coupled to two representative antibodies were compared to optimize separation of Foxp3+ from Foxp3− events. Foxp3 gates were set using two gating strategies based on CD127+CD25− “non‐Tregs” or based on isotype controls. For Foxp3 staining, the best conditions for fixation/permeabilization were obtained using the eBioscience Foxp3, Imgenex, BioLegend, and BD Foxp3 buffers. Comparing results from 10 subjects, 259D/C7, PCH101, 236A/E7, and 206D antibodies yielded statistically higher levels of Foxp3 cells than those by 150D and 3G3 antibodies (mean = 6.9, 5.1, 4.7, and 3.7% compared with 1.7, and 0.3% of CD25+Foxp3+ events within CD4+ cells, respectively). Importantly, the “nonspecificity” of some antibodies observed with a Foxp3 gate based on isotype controls could be eliminated by setting the Foxp3 gate on “non‐Tregs”. Better separation of Foxp3+ and Foxp3− populations was observed using the PCH101 clone coupled to Alexa647 compared with FITC or the 259D/C7 clone coupled to PE compared with Alexa488 fluorochrome. Foxp3 staining can be highly variable and depends on the choice of antibody/buffer pair and the fluorochrome used. Selecting the correct population for setting the Foxp3 gate is critical to avoid including non‐Tregs in the Foxp3+ gate. The experiments presented here will aid in optimization of flow cytometry staining panels to quantify Treg frequencies in humans. © 2009 International Society for Advancement of Cytometry
Histiocytic sarcoma with interdigitating dendritic cell differentiation: A case report with fine needle aspiration cytology and review of literature
Authors: Yang Grace C. H. , Besanceney Carrie E. , Tam Wayne 
Published: October 2009

AbstractA hybrid histiocytic sarcoma‐interdigitating dendritic cell sarcoma was found in a small perinephric lymph node of an asymptomatic 80‐year‐old man, who presented a year ago with two small foci of lung metastasis found during routine chest X‐ray. Fine needle aspiration cytology demonstrated interconnecting long and thin cell processes radiating from dendrite‐like neoplastic cells with oval, enlongated, reniform, and irregular nuclei with vesicular chromatin and distinct nucleoli. Histology showed spindled epitheliod and histiocytic cells with abundant, slightly eosinophilic cytoplasm with indistinct cell borders and forming fascicles in a vague storiform pattern with interspersed T‐lymphocytes. Immunohistochemically, the neoplastic cells were strongly positive for histiocytic markers: CD163, CD68, lysozyme, and PU.1, as well as strongly positive for dendritic cell markers: S100 and fascin, but were negative for CD1a (Langerhans cell marker), CD21/CD35 (follicular dendritic cell markers), B‐cell, and T cell markers. This case is compared to the four hybrid histiocytic‐dendritic sarcomas reported since 1983. Diagn. Cytopathol. 2010. © 2009 Wiley‐Liss, Inc.
Rational Synthesis, Structure, Magnetism and Electrochemistry of Mixed Iron–Nickel‐Containing Wells–Dawson‐Fragment‐Based Sandwich‐Type Polyoxometalates
Authors: Mbomekalle Israel M. , Mialane Pierre , Dolbecq Anne , Marrot Jérôme , Sécheresse Francis , Berthet Patrick , Keita Bineta , Nadjo Louis 
Published: October 2009

AbstractThe compound [Na2(H2O)2Ni2(As2W15O56)2]18–, Ni2As4, was synthesized and structurally characterized for the first time by X‐ray crystallography. This complex reacts with FeIII to form two new mixed‐metal sandwich‐type complexes, [Fe2(H2O)2Ni2(As2W15O56)2]14–, Fe2_Ni2As4, and [Fe(H2O)Ni(H2O)Ni2(As2W15O56)2]15–, FeNi_Ni2As4. In comparison, the synthesis of the previously reported complex [Na2(H2O)2Fe2(As2W15O56)2]16–, Fe2As4, was rationalized and improved, and the incorporation of NiII into the latter compound leads to the new mixed‐metal complex [Ni2(H2O)2Fe2(As2W15O56)2]14–, Ni2_Fe2As4. Complete characterization of these complexes by FTIR, UV/Vis, elemental analysis, magnetic measurements and X‐ray crystallography, wherever possible, clearly defined their electronic and structural properties. These complexes undergo multiple electron‐transfer processes and are effective in the electrocatalytic reduction of nitrite, dioxygen and hydrogen peroxide.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Si94– Anions in Solution – Structures of the Solvates Rb4Si9·4.75NH3 and [Rb(18‐crown‐6)]Rb3Si9·4NH3, and Chemical Bonding in Si94– (Eur. J. Inorg. Chem. 31/2009)
Authors: Joseph Stefanie , Suchentrunk Christof , Kraus Florian , Korber Nikolaus 
Published: October 2009

AbstractThe cover picture shows significant parts of the crystal structures of Rb(18‐crown‐6)Rb3Si9·4NH3 and Rb4Si9·4.75NH3. On the left, the coordination sphere of the nonasilicide anion in the 18‐crown‐6‐containing compound is shown. The second structural part shows the Rb‐ammonia‐nonasilicide strands found in the crystal structure of Rb4Si9·4.75NH3. The existence of these thermally labile ammoniates demonstrates that it is possible to extract Si94– anions from a ternary solid‐state material into a liquid ammonia solution without further oxidation. This opens the door to chemical transformations of bare electron‐rich silicon clusters. The ELF picture on the left shows that no evidence for three‐center, two‐electron bonding can be found, although the Wade–Mingos–Williams rules obviously apply for the prediction of the molecular shape of the anions. Details are discussed in the article by N. Korber et al. on p. 4641 ff.
Excretion characteristics of urinary 8‐hydroxydeoxyguanosine after dietary exposure to polycyclic aromatic hydrocarbons
Authors: Chien Yeh‐Chung , Yeh Chun‐Ting 
Published: October 2009

AbstractUrinary 8‐hydroxydeoxyguanosine (8‐OHdG) is considered a noninvasive marker for oxidative stress and also a marker of carcinogenic potential for compounds such as polycyclic aromatic hydrocarbons (PAHs). Although human studies have investigated urinary 8‐OHdG concentrations in PAH‐exposed workers and the general population, the background level and excretion kinetics of urinary 8‐OHdG in humans remain unclear. Two feeding experiments (consumption of barbecued meat of 15 and 30 g/kg for Experiments 1 and 2, respectively) were conducted to examine the excretion characteristics of urinary 8‐OHdG. All urine voided over 7 days was collected, but only first morning (∼8 A.M.) and last afternoon (∼5 P.M.) samples were analyzed for 8‐OHdG. Mean background urinary 8‐OHdG concentration was 4.76 μg/g creatinine. Statistically significant increases (P < 0.05) in urinary 8‐OHdG concentration were observed on the afternoon of the 3rd and 2nd days after barbecued meat consumption for Experiments 1 and 2, respectively. A pattern of diurnal fluctuation (P < 0.05) in 8‐OHdG excretion rate was evident, in that the excretion of 8‐OHdG was faster during the night than during the day. Additionally, significant (P < 0.05) and strong (r > 0.6) correlations were found between urinary 8‐OHdG measured 2–3 days after exposure to barbecued meat, and 1‐hydroxypyrene (1‐OHP) and 3‐hydroxy‐benzo[a]pyrene (3‐OHBaP) concentrations measured within a half day after such exposure. The current results demonstrate a lag in excretion of urinary 8‐OHdG relative to 1‐OHP and 3‐PHBaP after dietary PAH exposure. These relationships highlight the importance of sampling time when assessing PAH‐related DNA lesions through urinary 8‐OHdG. Environ. Mol. Mutagen. 2010. © 2009 Wiley‐Liss, Inc.
Antioxidant activity of essential oils of five spice plants widely used in a Mediterranean diet
Authors: Viuda‐Martos Manuel , Ruiz Navajas Yolanda , Sánchez Zapata Elena , Fernández‐López Juana , Pérez‐Álvarez José A. 
Published: October 2009

AbstractThe oxidative degradation of lipids is one of the main factors limiting the shelf‐life of food products. In recent years, several undesirable disorders have been detected as side‐effects of using commonly used synthetic antioxidants. Apart from their use as aroma additives in food, essential oils from aromatic plants have shown potential for use in small amounts in fat‐containing food systems to prevent or delay some types of chemical deterioration that occur during storage. Using a multiple‐method approach, the antioxidant activity of the essentials oils from several spices widely used in Mediterranean countries was tested: oregano (Origanum vulgare), thyme (Thymus vulgaris), rosemary (Rosmarinus officinalis), sage (Salvia officinalis) and clove (Syzygium aromaticum). Their total phenolic compound content was also determined. The clove essential oil had the highest amount of total phenols (898.89 mg/l GAE) and showed the highest percentage inhibition of DPPH radical (98.74%) and the highest FRAP value (1.47 TEAC). The thyme essential oil produced the highest percentage inhibition of TBARS (89.84%). All the essential oils studied were capable of chelating iron(II), the rosemary essential oil producing the highest effect (76.06%) in this respect. The oregano essential oil had the highest antioxidant activity index in the Rancimat test. Copyright © 2009 John Wiley & Sons, Ltd.
Global and local central non‐upwind finite volume schemes for hyperbolic conservation laws in porous media
Authors: Edwards Michael G. 
Published: October 2009

AbstractThe focus of this paper is on the development of convective flow approximation schemes that offer the benefits of an upwind formalism without actually upwinding. Development of robust schemes that remove or reduce upwind dependence would represent a significant step forward leading to a fundamental simplification of current methods for flow in porous media and reservoir simulation. Copyright © 2009 John Wiley & Sons, Ltd.
Soluble ions more than particulate cobalt‐alloy implant debris induce monocyte costimulatory molecule expression and release of proinflammatory cytokines critical to metal‐induced lymphocyte reactivity
Authors: Caicedo Marco S. , Pennekamp Peter H. , McAllister Kyron , Jacobs Joshua J. , Hallab Nadim J. 
Published: October 2009

AbstractAseptic osteolysis has been associated with excessive immune reactivity to particulate implant debris; however, innate and adaptive immune mechanisms that underlie implant debris reactivity remain incompletely understood. Although particulate debris has been implicated as the major type of implant debris mediating macrophage‐induced osteolysis, the degree to which metal ions affect a proinflammatory response (if at all) remains unknown. We hypothesized that both soluble and particulate metal implant debris will induce proinflammatory responses in human monocytes resulting in cytokine production and elevated expression of T cell costimulatory molecules, facilitating adaptive immune responses. We tested this hypothesis by characterizing the response of a human monocyte cell line (THP‐1), isolated primary human monocytes and PBMCs challenged with Co‐Cr‐Mo alloy particles and soluble cobalt, chromium, molybdenum, and nickel ions. Our results indicate that soluble cobalt, nickel, and molybdenum can induce monocyte up‐regulation of T cell costimulatory molecules (CD80, CD86, ICAM‐1) in human monocytes/macrophages. Furthermore, cobalt, molybdenum ions, and Co‐Cr‐Mo alloy particles similarly induce elevated secretion of IL‐1β, TNFα, and IL‐6. Antibody blockade of CD80 and CD86, crucial secondary molecules for adaptive responses, abrogated lymphocyte reactivity to metal challenge in metal reactive subjects. Also the addition of IL‐1 receptor antagonist (IL‐1ra), (which indirectly blocks pro‐IL‐1β and thus IL‐1β release), significantly reduced lymphocyte reactivity in metal‐reactive subjects. Thus, both soluble and particulate metal implant debris induce monocyte/macrophage proinflammatory responses that are metal and individual specific. This suggests metal‐induced up‐regulation of costimulatory molecules and proinflammatory cytokine production is necessary to induce lymphocyte activation/proliferation to metal implant debris. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res 2010
Community involvement and victimization at school: an analysis through family, personal and social adjustment
Authors: Jiménez Teresa Isabel , Musitu, Gonzalo , Ramos Manuel Jesús , Murgui Sergio 
Published: October 2009

AbstractThe present study analyzes the impact of adolescents' community involvement on victimization by peers at school through various indicators of family, personal and social adjustment (openness of communication with mother and father, life satisfaction, social self‐esteem, and loneliness). Participating in the project were 565 adolescents aged 11 to 18 (51% male) drawn from secondary schools in Spain. Statistical analyses were conducted using bivariate correlations, the t test and structural equation modeling. Results indicated an indirect and protective influence of community involvement, openness of communication with parents and life satisfaction on victimization by peers. There was also a direct protective effect of social self‐esteem and a direct risk effect of loneliness on victimization at school. Findings are discussed in light of the consideration that community involvement is a key factor in the promotion of other protective factors related to adolescent victimization at school. © 2009 Wiley Periodicals, Inc.
Building strong communities: an evaluation of a neighborhood leadership program in a diverse urban area
Authors: Ayón Cecilia , Lee Cheryl D. 
Published: October 2009

AbstractThe purpose of this study was to describe and evaluate an intervention used to train neighborhood leaders about community organizing and to enhance leadership skills. A mixed‐method design was used which included (a) a pre‐ and posttest assessment of 83 participants, and (b) qualitative descriptive interviews of 33 participants. Over half of the participants in the study were from ethnic minority groups (Latino or Cambodian). At posttest assessment, the participants improved in leadership skills (p=.001) and experience (p=.001) subscales. The qualitative interviews revealed that participants continued to be active in their communities by implementing neighborhood programs or starting community organizations. It is recommended that community practitioners and activists support neighborhood leadership programs to foster growth and enrichment in communities, and researchers/practitioners evaluate these projects with multiple methods to broaden scholarship in this important arena. © 2009 Wiley Periodicals, Inc.
HUI Mālama O Ke Kai: a positive prevention‐based youth development program based on native hawaiian values and activities
Authors: Hishinuma Earl S. , Chang, Janice Y. , Sy Angela , Greaney Malia F. , Morris Katherine A. , Scronce Ami C. , Rehuher, Davis , Nishimura Stephanie T. 
Published: October 2009

AbstractEvaluation of after‐school programs that are culturally and place‐based and promote positive youth development among minority and indigenous youths has not been widely published. The present evaluation is the first of its kind of an after‐school, youth‐risk prevention program called Hui Malāma O Ke Kai (HMK), that emphasizes Native Hawaiian values and activities to promote positive youth development for fifth and sixth graders (N=110) in a rural Native Hawaiian community. Results indicated positive gains on youth self‐reports in Native Hawaiian values, self‐esteem, antidrug use, violence prevention strategies, and healthy lifestyle in Year 1, and in family cohesion, school success, and violence prevention strategies in Year 2. Parent reports of their children indicated positive gains in selected domains. Implications include the support for a promising culturally appropriate program, expansion to middle‐school‐aged youths, and parent involvement. © 2009 Wiley Periodicals, Inc.
Bite force and performance in the durophagous bonnethead shark, Sphyrna tiburo
Authors: Mara Kyle R. , Motta Philip J. , Huber Daniel R. 
Published: October 2009

AbstractBite force, a measure of performance, can be used to link anatomical form and function. Earlier studies have shown bite force to have a significant influence on dietary constraints and ontogenetic shifts in resource utilization. The bonnethead shark, Sphyrna tiburo, is a durophagous member of the family Sphyrnidae. Its diet in South Florida waters consists almost entirely of blue crabs, which are crushed or ingested whole. This abundant coastal predator's feeding mechanism is specialized for the consumption of hard prey, including a modified biting pattern and molariform teeth. The goals of this research were to (1) characterize the mechanical function of the feeding mechanism of S. tiburo through biomechanical modeling of biting and in vivo bite force measurements; (2) compare the bite force of S. tiburo with those of other fishes; and (3) identify functional constraints on prey capture by comparing the bite force of S. tiburo with the fracture properties of its primary prey item, blue crabs. Maximum theoretical bite force ranged from 25.7 N anteriorly to 107.9 N posteriorly. S. tiburo has the second lowest mass specific bite force for any fish studied to date, and its posterior mechanical advantage of 0.88 is lower than other durophagous chondrichthyans, indicating that this independent evolutionary acquisition of durophagy was not accompanied by the associated morphological changes found in other durophagous cartilaginous fishes. Blue crab fracture forces (30.0–490.0 N) range well above the maximum bite force of S. tiburo, suggesting that prey material properties functionally constrain dietary ecology to some degree. J. Exp. Zool. 313A:95–105, 2010. © 2009 Wiley‐Liss, Inc.
Cycles and paths in edge‐colored graphs with given degrees
Authors: Abouelaoualim, A. , Das K. Ch. , de la Vega W. Fernandez , Karpinski M. , Manoussakis Y. , Martinhon C. A. , Saad R. 
Published: October 2009

AbstractSufficient degree conditions for the existence of properly edge‐colored cycles and paths in edge‐colored graphs, multigraphs and random graphs are investigated. In particular, we prove that an edge‐colored multigraph of order n on at least three colors and with minimum colored degree greater than or equal to ⌈(n+1)/2⌉ has properly edge‐colored cycles of all possible lengths, including hamiltonian cycles. Longest properly edge‐colored paths and hamiltonian paths between given vertices are considered as well. © 2009 Wiley Periodicals, Inc. J Graph Theory 64: 63–86, 2010
A note on regular Ramsey graphs
Authors: Alon Noga , Ben‐Shimon Sonny , Krivelevich Michael 
Published: October 2009

AbstractWe prove that there is an absolute constant C>0 so that for every natural n there exists a triangle‐free regular graph with no independent set of size at least \documentclass{article}\usepackage{amssymb}\usepackage{amsbsy}\usepackage[mathscr]{euscript}\footskip=0pc\pagestyle{empty}\begin{document}\({{C}}\sqrt{{{n}}\log{{n}}}\).\end{document} © 2009 Wiley Periodicals, Inc. J Graph Theory 64: 244–249, 2010
Hypersensitivity to foods: The adaptation phenomenon
Authors: Wilson William H. 
Published: October 2009

AbstractThe ability of certain patients with an allergic diathesis to adapt to the ingestion of specific foods to which they are allergic accounts for the present confusion relative to the role food hypersensitivity plays in clinical medicine. An understanding of the concept of adaptation in ingestant allergy, with its stimulatory and withdrawal periods, aids both patient and physician in appreciating the problems involved in the recognition of food hypersensitivity.Specific adaptation undergoes a chronological development from the non‐adapted stage through full adaptation to eventual maladaptation. Recognition of the stage of development present offers further diagnostic help in evaluation of the allergic patient.Reintroduction of a specific food into the diet after establishment of an induced nonadapted phase provides an accurate means of determining hypersensitivity to individual ingestants.
Noxious effects upon cochlear metabolism
Authors: Thalmann R. , Thalmann I. , Ise I. , Paloheimo S. 
Published: October 2009

AbstractThe influence of various toxic substances and of drugs with ototoxic side effects upon energy generation, energy utilization, and membrane processes of the cochlea were studied. None of the drugs tested interfered with energy generation to as great an extent as did anoxia or cyanide and 2,4‐dinitrophenol. Ouabain produced a pronounced interference with energy utilization of the stria vascularis. The “loop” diuretics ethacrynic acid and furosemide produced a reduction of energy utilization of a lesser degree than did ouabain. The “loop” diuretics do not seem to exert their toxic action upon strial Na+K+‐ATPase, but may act by interfering with strial adenylate cyclase. Aminoglycoside antibiotics and diuretic and nondiuretic mercurials seem to exert their primary noxious action upon cochlear function by interfering with membrane processes of the structures bounding the cochlear duct.
Malignant neoplasms of the nasal cavities and paranasal sinuses: (A retrospective study)
Authors: Jackson R. T. , Fitz‐Hugh G. S. , Constable William C. 
Published: October 2009

AbstractThis study presents a retrospective look at 115 patients evaluated here from 1957 to 1974. In this series, 57 percent were males and 43 percent females, 84 percent were Caucasian and 16 percent Negro. Lesions confined to or originating in the antrum made up 67 percent while non‐antral lesions were 33 percent. Mean age at diagnosis was 59.1 years. Smoking and drinking history did not appear to be contributory.Antral lesions were retrospectively staged according to Sisson's TNM classification. Sixteen tumor types were involved, with the most common being epidermoid. Diagnosis was most often made by intranasal or Cald‐well‐Luc biopsy. Most frequent symptoms, as well as earliest symptoms, were nasal obstruction, localized pain, and epistaxis. Average duration of symptoms was 6.4 months.Therapy was generally in the form of radiotherapy alone, preoperative radiotherapy and surgery, surgery and postoperative radiotherapy, or surgery alone. Local recurrences occurred in 44 percent of antral lesions and 50 percent of non‐antral lesions. Regional (cervical) nodal metastases developed in 25 percent of antral lesions and 11 percent of non‐antral lesions. Distant metastases developed in 30 percent of antral cases and 35 percent of non‐antral cases. Five‐year survival was 32 percent (35 percent determinate) for the total group. The more advanced the staging of the antral lesions, the worse the prognosis. Best survival figures were in the areas of preoperative radiotherapy and surgery at 38 percent (43 percent) and surgery alone at 56 percent (59 percent).