bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single Cell Isolation of Bacteria from Microbial Fuel Cells and Potomac River Sediment
Authors: Ringeisen B. R. , Lizewski S. E. , Fitzgerald L. A. , Biffinger J. C. , Knight C. L. , Crookes‐Goodson W. J. , Wu P. K. 
Published: March 2010

AbstractElectrochemically active bacteria (EAB) are prominently found in aquatic environmental sediment samples and wastewater streams, which are known to contain several different types of microorganisms. Even though microbial consortia are found to enhance both Coulombic efficiency and total power output in microbial fuel cells (MFCs), it is currently unknown how many different EAB contribute to current generation in these systems. It is also difficult to track the relative population of different species during MFC operation. We used biological laser printing (BioLP) to isolate different bacterium from complex environmental samples and MFC anolytes. BioLP can be used to print droplets containing a single cell directly from liquid culture, thereby enabling EAB to be sorted from unmodified environmental or MFC samples. Isolated species were identified through 16S rDNA analysis of pure cultures derived from the printed samples. These experiments demonstrate how cell printing can be used as a single‐step method to separate and identify microorganisms from complex environmental samples and operating MFCs.
Recalcitrant hypocalcemia in a lactating woman after total thyroidectomy for papillary thyroid carcinoma
Authors: Lassig Amy Anne D. , Donatelli Paul E. , Teknos Theodoros N. 
Published: March 2010

AbstractBackgroundThe risk of hypocalcemia after total thyroidectomy is well‐described in the literature. Recalcitrant hypocalcemia after thyroidectomy in a lactating woman is uncommon and may require multiple strategies to correct.MethodsWe present a case report of a lactating woman with papillary thyroid carcinoma requiring total thyroidectomy and neck dissections.ResultsPostoperatively the patient suffered from recalcitrant hypocalcemia which necessitated multiple medical maneuvers to rectify. The medical treatment required to correct the calcium levels is described herein.ConclusionWith the incidence of thyroid cancer on the rise and the increased identification of thyroid lesions during pregnancy, the number of lactating women undergoing thyroidectomy will likely increase. Surgeons performing thyroid surgery in this patient population should be aware of the risk of severe hypocalcemia and the tools necessary to correct it. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Enhanced angiogenesis of modified porcine small intestinal submucosa with hyaluronic acid‐poly(lactide‐co‐glycolide) nanoparticles: From fabrication to preclinical validation
Authors: Mondalek Fadee G. , Ashley Richard A. , Roth Christopher C. , Kibar Yusuf , Shakir Nabeel , Ihnat Michael A. , Fung Kar‐Ming , Grady Brian P. , Kropp Bradley P. , Lin Hsueh‐Kung 
Published: March 2010

AbstractHyaluronic acid‐poly(de‐co‐glycolide) nanoparticles (HA‐PLGA NPs) were synthesized to stabilize the porous structure of porcine small intestinal submucosa (SIS), to improve surface biocompatibility and to enhance performance in tissue regeneration. HA‐PLGA NPs were characterized for size, zeta potential, surface morphology, and HA loading. Human microvascular endothelial cells responded to HA‐PLGA NPs and HA‐PLGA modified SIS (HA‐PLGA‐SIS) with elevated cell proliferation. HA‐PLGA‐SIS significantly enhanced neo‐vascularization in an in ovo chorioallantoic membrane angiogenesis model. The angiogenic capability of the newly fabricated HA‐PLGA‐SIS was tested in a canine bladder augmentation model. Urinary bladder augmentation was performed in beagle dogs following hemi‐cystectomy using HA‐PLGA‐SIS. The regenerated bladder was harvested at 10 weeks post augmentation and vascularization was evaluated using CD31 immunohistochemical staining. Bladder regenerated with HA‐PLGA‐SIS had significantly higher vascular ingrowth compared to unmodified SIS. This study shows that HA‐PLGA NPs may represent a new approach for modifying naturally derived SIS biomaterials in regenerative medicine. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010
Vasostatin 1 activates eNOS in endothelial cells through a proteoglycan‐dependent mechanism
Authors: Ramella Roberta , Boero Ombretta , Alloatti Giuseppe , Angelone Tommaso , Levi Renzo , Gallo Maria Pia 
Published: March 2010

AbstractAccumulating evidences point to a significant role for the chromogranin A (CgA)‐derived peptide vasostatin 1 (VS‐1) in the protective modulation of the cardiovascular activity, because of its ability to counteract the adrenergic signal. We have recently shown that VS‐1 induces a PI3K‐dependent‐nitric oxide (NO) release by endothelial cells, contributing to explain the mechanism of its cardio‐suppressive and vasodilator properties. However, the cellular processes upstream the eNOS activation exerted by this peptide are still unknown, as typical high‐affinity receptors have not been identified. Here we hypothesize that in endothelial cells VS‐1 acts, on the basis of its cationic and amphipathic properties, as a cell penetrating peptide, binding to heparan sulfate proteoglycans (HSPGs) and activating eNOS phosphorylation (Ser1179) through a PI3K‐dependent, endocytosis‐coupled mechanism. In bovine aortic endothelial cells (BAE‐1 cells) endocytotic vesicles trafficking was quantified by confocal microscopy with a water‐soluble membrane dye; caveolin 1 (Cav1) shift from plasma membrane was studied by immunofluorescence staining; VS‐1‐dependent eNOS phosphorylation was assessed by immunofluorescence and immunoblot analysis. Our experiments demonstrate that VS‐1 induces a marked increase in the caveolae‐dependent endocytosis, (115 ± 23% endocytotic spots/cell/field in VS‐1‐treated cells with respect to control cells), that is significantly reduced by both heparinase III (HEP, 17 ± 15% above control) and Wortmannin (Wm, 7 ± 22% above control). Heparinase, Wortmannin, and methyl‐β‐cyclodextrin (MβCD) abolish the VS‐1‐dependent eNOS phosphorylation (PSer1179eNOS). These results suggest a novel signal transduction pathway for endogenous cationic and amphipathic peptides in endothelial cells: HSPGs interaction and caveolae endocytosis, coupled with a PI3K‐dependent eNOS phosphorylation. J. Cell. Biochem. 110: 70–79, 2010. © 2010 Wiley‐Liss, Inc.
Functional expression of double‐stranded RNA‐dependent protein kinase in rat intestinal epithelial cells
Authors: Sato Nagahiro , Morimoto Hiroyuki , Baba Ryoko , Nakamata Junichi , Doi Yoshiaki , Yamaguchi Koji 
Published: March 2010

AbstractIntestinal epithelial cells (IECs) are exposed to external environment, microbial and viral products, and serve as essential barriers to antigens. Recent studies have shown that IECs express Toll‐like receptors (TLRs) and respond to microbial components. The antimicrobial and antiviral barriers consist of many molecules including TLRs. To investigate the further component of this barrier in intestine, we examined the expression of double‐stranded RNA‐dependent protein kinase (PKR). PKR is a player in the cellular antiviral response and phosphorylates α‐subunit of the eukaryotic translation initiation factor 2 (eIF‐2α) to block protein synthesis and induces apoptosis. In this study, we showed that the expression of PKR was restricted to the cytoplasm of absorptive epithelial cells in the intestine of adult rat. We also demonstrated that PKR was expressed in the cultured rat intestinal epithelial cells (IEC‐6). The level of PKR protein expression and the activity of alkaline phosphatase (ALP) increased in the cultured IEC‐6 cells in a time‐dependent manner. Inhibition of PKR by the 2‐aminopurine treatment decreased ALP activity in the IEC‐6 cells. Treatment of IEC‐6 cells with synthetic double‐stranded RNA (dsRNA) induced cell death in a dose‐dependent manner. The addition of hydrocortisone also provoked suppression of PKR expression and ALP activity. This modulation might be mediated by signal transducers and activators of transcription‐1 (STAT‐1) protein. We concluded that PKR is expressed in IECs as potent barriers to antigens and is a possible modulator of the differentiation of rat IECs. J. Cell. Biochem. 110: 104–111, 2010. © 2010 Wiley‐Liss, Inc.
Replication initiation and DNA topology: The twisted life of the origin
Authors: Rampakakis E. , Gkogkas C. , Di Paola D. , Zannis‐Hadjopoulos M. 
Published: March 2010

AbstractGenomic propagation in both prokaryotes and eukaryotes is tightly regulated at the level of initiation, ensuring that the genome is accurately replicated and equally segregated to the daughter cells. Even though replication origins and the proteins that bind onto them (initiator proteins) have diverged throughout the course of evolution, the mechanism of initiation has been conserved, consisting of origin recognition, multi‐protein complex assembly, helicase activation and loading of the replicative machinery. Recruitment of the multiprotein initiation complexes onto the replication origins is constrained by the dense packing of the DNA within the nucleus and unusual structures such as knots and supercoils. In this review, we focus on the DNA topological barriers that the multi‐protein complexes have to overcome in order to access the replication origins and how the topological state of the origins changes during origin firing. Recent advances in the available methodologies to study DNA topology and their clinical significance are also discussed. J. Cell. Biochem. 110: 35–43, 2010. © 2010 Wiley‐Liss, Inc.
Pneumatically assisted desorption/ionization: 1. Some thoughts on the possible ionization mechanism(s)
Authors: Zivolic Fabio , Zancanaro Flavio , Favretto Donata , Ferrara Santo Davide , Seraglia Roberta , Traldi Pietro 
Published: March 2010

AbstractWe have observed that spraying solvent droplets on a zopiclone tablet produced MH+ ions also in the absence of any electrical field and without the addition of organic acids to the sprayed solvent. The choice of a drug tablet as test bench has been done for the signal stability, higher than that observed when the drug is directly placed on a stainless steel surface. This behavior indicates that the formation of MH+ ions is mainly due to pneumatical effects and the results are discussed with respect to those obtained by other research groups. Different mechanisms contributing to MH+ production under these conditions are proposed and discussed. The local heating of the solvent thin layer present on the surface has been calculated and the small temperature increase (and the consequent small decrease of pKa value) suggests that this effect can play only a minor role. However, different solvents have been employed for studying this aspect and, quite surprisingly, the best results have been obtained with acetonitrile (ACN). Experiments performed by spraying CD3CN showed again the formation of MH+ and not MD+, and this excludes the role of ACN as protonating medium. A further thought was stimulated by the behavior observed by varying the sheath gas (N2) flow, showing that the MH+ ion intensity increases by increasing the flow. Side effects related to the highest kinetic energy of the spraying droplets can be considered, but an active role of N2 in the MH+ formation could be taken into account, by considering the possible ionization of N2 by collisional phenomena. The N2+• ions could undergo a charge–exchange reaction with analyte molecules leading to a short‐lived odd electron ion which behaves as protonating media for neutral molecules. The above‐described mechanism does not require either the presence of electrical fields nor the addition of organic acid to the sprayer solvent and can give a rationale for what was observed when only pneumatical conditions are employed. Copyright © 2010 John Wiley & Sons, Ltd.
A Raman spectroscopic study on the allocation of ammonium adsorbing sites on H2Ti3O7 nanofibre and its structural derivation during calcination
Authors: Liu Hongwei , Zheng Zhanfeng , Yang Dongjiang , Waclawik Eric , Ke Xuebin , Zhu Huaiyong , Palmer Sara , Frost Ray L. 
Published: March 2010

AbstractThe adsorption behaviour of ammonium ions and the structural features of layered proton trititanate were characterised by using Raman spectroscopy, X‐ray diffraction (XRD) and transmission electron microscopy. It revealed that the intensity of the Raman band at 309 cm−1, assigned to very long TiO bonds (0.22 nm), reduced, whereas the band at 890 cm−1, assigned to very short TiO bonds (0.17 nm), increased slightly after the adsorption of ammonium ions (NH4+). The adsorption of ammonium ions enlarged the interlayer distance of the (200) plane. Ammonium ions were located at the corner of the TiO6 octahedral slabs. This was further confirmed by XRD, with an increased intensity of the (201) plane being observed. Copyright © 2010 John Wiley & Sons, Ltd.
Far‐infrared spectroscopy, the wavenumbers of metal–ligand vibrations and the characterisation of inorganic pigments
Authors: Clark Robin J. H. 
Published: March 2010

AbstractExtensive far‐infrared studies of inorganic materials and pigments in the early 1960s are highlighted and related to the many subsequent Raman studies of the same materials, studies which led to the formulation of Raman spectral libraries for use in conservation science. The need for complementary infrared/far‐infrared libraries is recognised. Copyright © 2010 John Wiley & Sons, Ltd.
Multidetector computed tomography for preoperative prediction of postsurgical prognosis of patients with extrahepatic biliary cancer
Authors: Kobayashi Shogo , Nagano Hiroaki , Marubashi Shigeru , Wada Hiroshi , Eguchi Hidetoshi , Takeda Yutaka , Tanemura Masahiro , Kim Tonsok , Doki Yuichiro , Mori Masaki 
Published: March 2010

AbstractBackgroundPreoperative prognostic information to select a treatment strategy is important especially in patients who need highly aggressive surgery, such as those with biliary cancer. We evaluated various prognostic factors and non‐curative surgical factors using multidetector computed tomography (MDCT).MethodsWe retrospectively analyzed 71 patients who underwent MDCT preoperatively and were scheduled for surgical resection of biliary cancer. For MDCT diagnosis, we used MDCT‐based classification equivalent to the surgical and pathological classification of the Japanese Society of Biliary Surgery. We evaluated MDCT‐related prognostic factors and non‐curative surgical factors and compared these factors with pathological results.ResultsMDCT‐diagnosed category T (primary tumor invasion) included both prognostic factors and non‐curative surgical factors but not category N (lymph node metastasis). Multivariate analysis identified MDCT‐based suspected arterial invasion as an independent prognostic factor. In patients suspected of arterial invasion by MDCT, the 3‐year overall survival rate was only 39% and the curative resection ratio was only 33%, because of the high positive surgical dissected margin.ConclusionMDCT‐based suspected arterial invasion is a predictor of poor prognosis after surgery for biliary cancer and represents a non‐curative surgical factor associated with positive dissected margin. J. Surg. Oncol. 2010; 101:376–383. © 2010 Wiley‐Liss, Inc.
Is FDG‐PET suitable for evaluating neoadjuvant therapy in non‐small cell lung cancer? Evidence with systematic review of the literature
Authors: Rebollo‐Aguirre Ángel C. , Ramos‐Font Carlos , Villegas Portero Román , Cook Gary J.R. , Llamas Elvira José Manuel , Romero Tabares Antonio 
Published: March 2010

AbstractBackgroundNeoadjuvant therapy response assessment is crucial in patients with non‐small cell lung cancer (NSCLC). FDG‐PET has emerged as a valuable tool for defining therapy response assessment in other tumours.AimTo systematically review publications appearing in the literature describing induction therapy response assessment with FDG‐PET in NSCLC.MethodsWe performed a bibliographic search and selected only prospective studies in order to include the highest levels of evidence.ResultsNine of 497 potentially relevant publications were selected. The ranges of sensitivity, specificity, positive predictive value and negative predictive value for primary tumour response assessment were 80–100%, 0–100%, 42.9–100%, and 66.7–100%, respectively. Pooling data for N2 restaging after neoadjuvant response the overall sensitivity was 63.8% (95% CI, 53.3–73.7%) and overall specificity was 85.3% (95% CI, 80.4–89.4%).ConclusionThe results of the analysis do not support the use of FDG‐PET as the only re‐assessment tool for mediastinal lymph node evaluation for routine clinical use. FDG‐PET seems to predict primary tumour response to induction therapy but it could not be shown by pooling analysis. J. Surg. Oncol. 2010; 101:486–494. © 2010 Wiley‐Liss, Inc.
Effect of vascular endothelial growth factor on nasal vascular permeability
Authors: Matsune Shoji , Ohori Junichiro , Yoshifuku Kousuke , Kurono Yuichi 
Published: March 2010

AbstractObjectives/Hypothesis:One of the major functions of vascular endothelial growth factor (VEGF) is increasing vascular permeability. We previously reported that VEGF is present in nasal secretions in rhinosinusitis, particularly in allergic rhinitis, and is hyperproduced immediately after antigen provocation. However, its function in nasal mucosa has not yet been investigated. This study was designed to estimate the increased vascular permeability by VEGF in nasal mucosa and to discuss its possible role in allergic rhinitis.Study Design:Prospective animal experiment.Methods:Hartley guinea pigs were used. Ethyl carbamate was injected into the abdomen for anesthesia, followed by intravenous injection of Evans blue into the femoral vein. Phosphate buffered saline, histamine, or VEGF was then applied to nasal cavities. Nasal mucosa was harvested after saline reflux from heart with phlebotomy by amputating the abdominal aorta. Tissue samples were soaked in formamide for 12 hours at 63°C to extract dye exudates from nasal mucosa. To analyze vascular permeability, dye was quantified by colorimetry.Results:VEGF increased vascular permeability in nasal mucosa, and was more than 1 × 106 times more potent than histamine on the basis of molar units. Although increased vascular permeability by VEGF was not inhibited by histamine 1 receptor antagonist, it was markedly inhibited by anti‐VEGF receptor antibodies.Conclusions:VEGF increased vascular permeability to a greater degree than histamine in nasal mucosa via a different pathway. Regulating VEGF may be a new therapeutic option for persistent nasal symptoms in allergic rhinitis. Laryngoscope, 2010
Functional outcomes of fibula and osteocutaneous forearm free flap reconstruction for segmental mandibular defects
Authors: Virgin Frank W. , Iseli Tim A. , Iseli Claire E. , Sunde Jumin , Carroll William R. , Magnuson Jeffery S. , Rosenthal Eben L. 
Published: March 2010

AbstractObjective/Hypothesis:To demonstrate that the osteocutaneous radial forearm free flap provides equivalent functional outcomes and improved morbidity compared to the fibular free flap in mandibular reconstruction.Study Design:Retrospective review.Methods:There were 168 patients requiring free flap reconstruction of segmental mandibular defects between January 2001 and December 2008. Mean follow‐up was 31 months for fibula free flap (FFF) (n = 117) and 20 months for osteocutaneous radial forearm free flaps (OCRFFF) (n = 51), reflecting an increasing use of forearms.Results:OCRFFF were more commonly used in older patients (mean 63.7 years vs. 59 years, P = .03). The majority (96.2%) of reconstruction was for malignant pathology. Flap failure was 3.4% for the fibula group and 3.9% in the forearm group. Malunion was infrequent (2.0% OCRFFF, 6.0% FFF, P = .26). Donor site complications were higher in the FFF group (4.3%) versus none in the OCRFF group (P = .13). Despite a high rate of long‐term survival in this patient population (75% at 5 years for carcinoma), dental implants were rarely placed (2.3% of patients) and were more common in forearm than fibula free flaps. Functional outcomes demonstrated no significant difference between groups with respect to oral diet (FFF 72.6% vs. OCRFFF 79.1%, P = .49) or retained enterogastric feeding tube (20.9% OCRFFF vs. 27.4% FFF, P = .49).Conclusions:Osteocutaneous radial forearm flaps provide comparable functional outcomes with less morbidity compared to fibula free flaps for selected segmental mandibulectomy defects. The overall dental implantation rate was low and more commonly performed in osteocutaneous radial forearm flaps compared to fibula flaps. Laryngoscope, 2010
Recovery of soil microbial community structure after fire in a sagebrush‐grassland ecosystem
Authors: Dangi S. R. , Stahl P. D. , Pendall E. , Cleary M. B. , Buyer J. S. 
Published: March 2010

AbstractRecovery of the soil microbial community after fire in a sagebrush‐grassland ecosystem was examined using a chronosequence of four sites ranging in time since fire from 3–39 years. The successional stage communities examined included Recent Burn (3 years since fire, ysf), Establishment (7 ysf), Expansion (21 ysf), and Mature (39 ysf). Aboveground standing plant biomass increased with time since disturbance to the Mature stage where sagebrush became dominant over herbaceous species. Phospholipid fatty acid (PLFA) analysis was used to characterize the microbial community structure. Soil microbial community productivity generally appeared to be similar to the Mature site soil (39 ysf) within 7 years of fire. Diversity of PLFAs detected in soils, at both depths, increased from a low value of 29 at the Recent site to a high of 37 at the Establishment site and then decreased again to 31 at the Mature stage site. Canonical variates analysis indicated important disparities in microbial community structure at the four sites. Greatest disparities were observed in microbial community structure between the Recent and Establishment stages but greater similarity between the Recent stage and the sagebrush dominated Mature stage. This study emphasizes both short‐term and long‐term changes in the belowground community and suggests that soil microbial communities are highly resilient to disturbances after prescribed fire. Copyright © 2010 John Wiley & Sons, Ltd.
Synthesis and Structure/Property Relationships of Regioselective 2‐O‐, 3‐O‐ and 6‐O‐Ethyl Celluloses
Authors: Kamitakahara Hiroshi , Funakoshi Toshihiro , Nakai Shinji , Takano Toshiyuki , Nakatsubo Fumiaki 
Published: March 2010

AbstractRegioselectively ethylated celluloses, 2‐O‐ (1), 3‐O‐ (2), and 6‐O‐ethyl‐ (3) celluloses were synthesized via ring‐opening polymerization of glucopyranose orthopivalate derivatives. The number‐average degrees of polymerization (DPns) of compounds 1 and 2 were calculated to be 10.6 and 49.4, respectively. Three kinds of compound 3 with different DPns were prepared: DPns = 12.9 (3‐1), 60.3 (3‐2), and 36.1 (3‐3). The 2‐O‐, 3‐O‐, and 6‐O‐ethylcelluloses were soluble in water, confirmed by NMR analysis. Furthermore, the 3‐O‐ (2), and 6‐O‐ethyl‐ (3‐2) celluloses showed thermo‐responsive aggregation behavior and had a lower critical solution temperature (LCST) at about 40 °C and 70 °C, respectively, based on the results from turbidity tests and DSC measurements. The 6‐O‐ethyl‐cellulose (3‐3) with DPn = 36.1 and DPw = 54.6 showed gelation behavior over approx 70 °C, whereas the 6‐O‐ethyl‐celluloses 3‐1 and 3‐2 with lower and higher molecular weight, such as DPns 12.9 and 60.3, did not show gelation behavior at this temperature. It was revealed that the position of ethyl group affected the phase transition temperature. According to our experiments, the 3‐O‐ethyl and 6‐O‐ethyl groups along the cellulose chains caused the thermo‐responsive property of their aqueous solutions. The appropriate DP of the regioselective 6‐O‐ethyl‐cellulose existed for gelation of the aqueous solution.magnified image