bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of novel 1,4‐benzoxazine compounds that are protective in tissue culture and in vivo models of neurodegeneration
Authors: Wang Lulu , Ankati Haribabu , Akubathini Shashidhar Kumar , Balderamos Michael , Storey Chelsey A. , Patel Anish V. , Price Valerie , Kretzschmar Doris , Biehl Edward R. , D'Mello Santosh R. 
Published: February 2010

AbstractNeurodegenerative diseases such as Alzheimer's disease, Parkinson's disease, and Huntington's disease and conditions such as ischemic stroke affect millions of individuals annually and exert an enormous financial burden on society. A hallmark of these conditions is the abnormal loss of neurons. Currently, there are no effective strategies to prevent neuronal death in these pathologies. We report that several 2‐arylidine and 2‐hetarylidin derivatives of the 1,4‐benzoxazines class of compounds are highly protective in tissue culture models of neurodegeneration. Results obtained using pharmcalogical inhibitors indicate that neuroprotection by these compounds does not involve the Raf–MEK–ERK or PI‐3 kinase–Akt signaling pathways nor other survival‐promoting molecules such as protein kinase A (PKA), calcium calmodulin kinase A (CaMK), and histone deacetylases (HDACs). We tested one of these compounds, (Z)‐6‐amino‐2‐(3′,5′‐dibromo‐4′‐hydroxybenzylidene)‐2H‐benzo[b][1,4]oxazin‐3(4H)‐one, designated as HSB‐13, in the 3‐nitropropionic acid (3‐NP)‐induced mouse model of Huntington's disease. HSB‐13 reduced striatal degeneration and improved behavioral performance in mice administered with 3‐NP. Furthermore, HSB‐13 was protective in a Drosophila model of amyloid precursor protein (APP) toxicity. To understand how HSB‐13 and other 1,4‐benzoxazines protect neurons, we performed kinase profiling analyses. These analyses showed that HSB‐13 inhibits GSK3, p38 MAPK, and cyclin‐dependent kinases (CDKs). In comparison, another compound, called ASK‐2a, that protects cerebellar granule neurons against low‐potassium‐induced death inhibits GSK3 and p38 MAPK but not CDKs. Despite its structural similarity to HSB‐13, however, ASK‐2a is incapable of protecting cortical neurons and HT22 cells against homocysteic acid (HCA)‐induced or Aβ toxicity, suggesting that protection against HCA and Aβ depends on CDK inhibition. Compounds described in this study represent a novel therapeutic tool in the treatment of neurodegenerative diseases. © 2010 Wiley‐Liss, Inc.
Local isoform‐specific NOS inhibition: A promising approach to promote motor function recovery after nerve injury
Authors: Moreno‐López Bernardo 
Published: February 2010

AbstractPhysical injury to a nerve is the most frequent cause of acquired peripheral neuropathy, which is responsible for loss of motor, sensory and/or autonomic functions. Injured axons in the peripheral nervous system maintain the capacity to regenerate in adult mammals. However, after nerve transection, stumps of damaged nerves must be surgically joined to guide regenerating axons into the distal nerve stump. Even so, severe functional limitations persist after restorative surgery. Therefore, the identification of molecules that regulate degenerative and regenerative processes is indispensable in developing therapeutic tools to accelerate and improve functional recovery. Here, I consider the role of nitric oxide (NO) synthesized by the three major isoforms of NO synthases (NOS) in motor neuropathy. Neuronal NOS (nNOS) seems to be the primary source of NO that is detrimental to the survival of injured motoneurons. Endothelial NOS (eNOS) appears to be the major source of NO that interferes with axonal regrowth, at least soon after injury. Finally, NO derived from inducible NOS (iNOS) or nNOS is critical to the process of lipid breakdown for Wallerian degeneration and thereby benefits axonal regrowth. Specific inhibitors of these isoforms can be used to protect injured neurons from degeneration and promote axonal regeneration. A cautious proposal for the treatment of acquired motor neuropathy using therapeutic tools that locally interfere with eNOS/nNOS activities seems to merit consideration. © 2010 Wiley‐Liss, Inc.
Down‐expression of PGC‐1α partially mediated by JNK/c‐Jun through binding to CRE site during apoptotic procedure in cerebellar granule neurons
Authors: Liang Jingyao , Yang Yi , Zhu Xiaonan , Wang Xuelan , Chen Ruzhu 
Published: February 2010

AbstractIn eukaryotes, mitochondria are critical for cellular bioenergetics and mediating apoptosis. The transcriptional coactivator peroxisome proliferator‐activated receptor gamma coactivator 1alpha (PGC‐1α) is an important regulator of mitochondrial biogenesis and function. However, the role of PGC‐1α in neuronal apoptosis and its regulation by apoptotic pathway are still unknown. We demonstrated that PGC‐1α expression was down‐regulated in cerebellar granule neurons(CGNs) after activation of the JNK/c‐Jun pathway by potassium deprivation. Overexpression of PGC‐1α partially protected CGNs from potassium deprivation‐induced apoptosis. JNK‐specific inhibitors, SP600125 and CEP11004, partially blocked the inhibitory effects of JNK on PGC‐1α expression and its promoter activity. Furthermore, ChIP assays revealed that c‐Jun was able to bind to the CRE site (‐188 to ‐180) in the PGC‐1α promoter. In conclusion, these results suggest that down‐expression of PGC‐1α partially mediated by activation of JNK/c‐Jun may be through the binding of c‐Jun to the CRE site in the PGC‐1α promoter, and it might be involved in potassium deprivation‐induced apoptosis in CGNs. © 2010 Wiley‐Liss, Inc.
Caspases‐2 and ‐8 are involved in the presenilin1/γ‐secretase‐dependent cleavage of amyloid precursor protein after the induction of apoptosis
Authors: Chae San Sook , Yoo Chul Bae , Jo Chulman , Yun Sang‐Moon , Jo Sangmee Ahn , Koh Young Ho 
Published: February 2010

AbstractThe presenilin/γ‐secretase protease cleaves many type‐I membrane proteins, including the amyloid β‐protein (Aβ) precursor (APP). Previous studies have shown that apoptosis induces alterations in Aβ production in a caspase‐dependent manner. Here, we report that staurosporine (STS)‐induced apoptosis induces caspase‐8 and/or‐2‐dependent γ‐secretase activation. Blocking of caspase activity with caspase‐8 inhibitor z‐IETD‐fmk, and caspase‐2 inhibitor z‐VDVAD‐fmk reduced Aβ production by STS in H4 cells expressing the Swedish mutant of APP (HSW) or APP‐C99 (H4‐C99). There was no inhibitory effect of other caspases (‐1, ‐3, ‐5, ‐6, ‐9) on Aβ production by STS. This finding was further supported by evidence that siRNA transfection, depleting caspase‐2 or ‐8 levels, lowered Aβ production in HSW and H4‐C99 cells without affecting expression of APP or γ‐secretase complex. In addition, Aβ production by STS was decreased by JNK inhibitors, SP600125. These results suggest that caspase‐2 and/or ‐8 is involved in presenilin/γ‐secretase activation and Aβ production in apoptosis. © 2010 Wiley‐Liss, Inc.
What motivates OCB? Insights from the volunteerism literature
Authors: Lavelle James J. 
Published: February 2010

AbstractThis paper argues that taking a functionalist perspective can contribute to a more complete understanding of why employees engage in organizational citizenship behavior (OCB). Informed by the volunteerism literature, a number of other‐oriented and self‐oriented functions are identified as predictors of OCB including value‐expression, social, and career‐related motives. Copyright © 2010 John Wiley & Sons, Ltd.
Molecular diversity of peanut‐nodulating rhizobia in soils of Argentina
Authors: Bogino Pablo , Banchio Erika , Giordano Walter 
Published: February 2010

AbstractRSα sequencing is a valuable tool for identification of bacterial strains, and for evaluating the genetic structure of indigenous rhizobial populations. The purpose of this study was to evaluate, qualitatively, the presence or absence of RSα fragment in peanut‐nodulating strains isolated from plants grown at four sites in central Argentina. RSα fragment was found in only three of 26 indigenous strains, and in one of three inoculant strains analyzed. In contrast to results from studies of other symbiotic nitrogen‐fixing bacteria, such as soybean‐nodulating strains, no correlation was found between generation time and presence of RSα sequence. Phylogenetic analysis of the 16S rRNA gene sequence grouped peanut‐nodulating strains into two clusters, Bradyrhizobium japonicum vs. B. elkanii, and showed divergence among strains positive for RSα sequence. Our results confirm the genetic diversity previously reported for various peanut‐nodulating rhizobial strains, and indicate that the RSα fragment is not applicable as a marker or tool for competition assays at the field or ecological level. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Effect of salinity on denitrification under limited single carbon source by Marinobacter sp. isolated from marine sediment
Authors: Nakano Miyo , Inagaki Tadashi , Okunishi Suguru , Tanaka Reiji , Maeda Hiroto 
Published: February 2010

AbstractMarinobacter comprises Gram‐negative, aerobic, motile, and rod‐shaped bacteria within the γ‐subclass of the Proteobacteria and is known to be halophilic or halotolerant, heterotrophic neutrophile. Two strains classified as belonging to Marinobacter, named PAD‐2 and SeT‐1, were isolated from marine sediment. The most closely related species of PAD‐2 and SeT‐1 are M. alkaliphilus and M. guinea, respectively. The strain PAD‐2 exhibited remarkably higher denitrification at concentrations of 0.5 to 1 M NaCl (3–6% w/w) than at other salinities (2 and 3 M NaCl, 12–18% w/w), and optimal denitrification was observed in media with 0.5 M NaCl. The effect of pH on denitrification by strain PAD‐2 was also examined, and the optimum denitrification occurred at neutral pH rather than under alkaline conditions. Overall, strain PAD‐2 appears to be a novel halotolerant species belonging to the genus Marinobacter that shares many characteristics, such as substrate utilization profile and optimum NaCl concentration for growth with M. alkaliphilus. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Combinatorial assessment on dominance and informative diversity of PGPR from rhizosphere of Jatropha curcas L.
Authors: Jha Chaitanya Kumar , Patel Dhara , Rajendran Narayanan , Saraf Meenu 
Published: February 2010

AbstractThe extensive and enduring challenges in soil microbiology depend on the development of efficient methods to be acquainted with the types of microbes present in soil, and to determine the functional performance of the overall microbial groups in situ. This study aims to investigate the combined uses of species richness and diversity as well as to estimate the combinatorial effect of species richness and diversity in order to understand their role and distribution in their habitat. To achieve this objective a study was designed targeting the rhizosphere of Jatropha curcas L. which were planted in various soil conditions on five distinctive sites of Gujarat state (India). These sites were constantly monitored and studied for the species richness and evenness (“heterogeneity”). The isolates were checked for their PGPR potentials like Phosphate solubilisation, Siderophore production, Indole acetic acid production, ACC deaminase production, HCN production, EPS production and Ammonia production. The results obtained were used to calculate richness, evenness and diversity indices. Results reveal the total heterogeneity in the site of fertile Jatropha rhizosphere (GS4) as well as sodic soil site (GS5) than other three sites. Absence of equitability under the selected and defined condition was also observed in GS4 and GS5 sites. The combinatorial estimates provide the information on their distribution and roles in the habitat. In particular, such an empirical relationship from a single rhizosphere of a distinctive species Jatropha is useful to test diversity predictions in natural sites, and further it can be applied to either by performing trials over larger spatial and temporal scales or by conducting correlational studies of biodiversity gradients. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Chondroitin‐6‐sulfate incorporation and mechanical stimulation increase MSC‐collagen sponge construct stiffness
Authors: Kinneberg Kirsten R.C. , Nirmalanandhan Victor S. , Juncosa‐Melvin Natalia , Powell Heather M. , Boyce Steven T. , Shearn Jason T. , Butler David L. 
Published: February 2010

AbstractUsing functional tissue engineering principles, our laboratory has produced tendon repair tissue which matches the normal patellar tendon force‐displacement curve up to 32% of failure. This repair tissue will need to withstand more strenuous activities, which can reach or even exceed 40% of failure force. To improve the linear stiffness of our tissue engineered constructs (TECs) and tissue engineered repairs, our lab is incorporating the glycosaminoglycan chondroitin‐6‐sulfate (C6S) into a type I collagen scaffold. In this study, we examined the effect of C6S incorporation and mechanical stimulation cycle number on linear stiffness and mRNA expression (collagen types I and III, decorin and fibronectin) for mesenchymal stem cell (MSC)‐collagen sponge TECs. The TECs were fabricated by inoculating MSCs at a density of 0.14 × 106 cells/construct onto pre‐cut scaffolds. Primarily type I collagen scaffold materials, with or without C6S, were cultured using mechanical stimulation with three different cycle numbers (0, 100, or 3,000 cycles/day). After 2 weeks in culture, TECs were evaluated for linear stiffness and mRNA expression. C6S incorporation and cycle number each played an important role in gene expression, but only the interaction of C6S incorporation and cycle number produced a benefit for TEC linear stiffness. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1092–1099, 2010
Silicon in beer and brewing
Authors: Casey Troy R , Bamforth Charles W 
Published: February 2010

AbstractBACKGROUND: It has been claimed that beer is one of the richest sources of silicon in the diet; however, little is known of the relationship between silicon content and beer style and the manner in which beer is produced. The purpose of this study was to measure silicon in a diversity of beers and ascertain the grist selection and brewing factors that impact the level of silicon obtained in beer.RESULTS: Commercial beers ranged from 6.4 to 56.5 mg L−1 in silicon. Products derived from a grist of barley tended to contain more silicon than did those from a wheat‐based grist, likely because of the high levels of silica in the retained husk layer of barley. Hops contain substantially more silicon than does grain, but quantitatively hops make a much smaller contribution than malt to the production of beer and therefore relatively less silicon in beer derives from them. During brewing the vast majority of the silicon remains with the spent grains; however, aggressive treatment during wort production in the brewhouse leads to increased extraction of silicon into wort and much of this survives into beer.CONCLUSION: It is confirmed that beer is a very rich source of silicon. Copyright © 2010 Society of Chemical Industry
Subjective sleep quality in women experiencing intimate partner violence: Contributions of situational, psychological, and physiological factors
Authors: Woods Stephanie J. , Kozachik Sharon L. , Hall Rosalie J. 
Published: February 2010

AbstractThis study, guided by an adaptation of the theory of unpleasant symptoms, examined the complex relationships of childhood maltreatment, intimate partner violence (IPV), posttraumatic stress disorder (PTSD), depression, and physical health symptoms with global sleep quality and disruptive nighttime behaviors. Data were analyzed using covariance structure analysis. A convenience sample of 157 women currently experiencing IPV was recruited from crisis shelters and community agencies. Findings provide empirical support that women concurrently experiencing PTSD, depression, and stress‐related physical health symptoms demonstrated poor global sleep quality and frequent disruptive nighttime behaviors. Posttraumatic stress disorder and stress health symptoms functioned as mediators of childhood maltreatment and IPV effects on both global sleep quality and disruptive nighttime behaviors, but depression did not.
Slow wave phenomena in photonic crystals
Authors: Figotin A. , Vitebskiy I. 
Published: February 2010

AbstractSlow light in photonic crystals and other periodic structures is associated with stationary points of the photonic dispersion relation, where the group velocity of light vanishes. It is shown that in certain cases, the vanishing group velocity is accompanied by the so‐called frozen mode regime, when the incident light can be completely converted into the slow mode with huge diverging amplitude. The frozen mode regime is a qualitatively new wave phenomenon – it does not reduce to any known electromagnetic resonance. Formally, the frozen mode regime is not a resonance, in a sense that it is not particularly sensitive to the size and shape of the photonic crystal. The frozen mode regime is more robust and powerful, compared to any known slow‐wave resonance. It has much higher tolerance to absorption and structural imperfections.
Surface electromagnetic waves: A review
Authors: Polo J.A. , Lakhtakia A. 
Published: February 2010

AbstractFrom the beginning of the 20th century, researchers have been interested in surface electromagnetic waves guided by planar interfaces of dissimilar mediums. Much initial research on surface‐plasmon waves and surface‐plasmon‐polariton waves stemmed from theoretical curiosity, but the development of new experimental techniques propelled commercial exploitation for optical sensors of chemical and biological species. Additional surface waves called Dyakonov waves, Tamm waves, and Dyakonov–Tamm waves have emerged during the last 25 years. Experimental observation of Dyakonov waves was reported only in 2009, but the rapid development of experimental apparatus during the 1990s suggests that theoretical predictions will soon be evaluated experimentally, leading to further development of optical detection systems and optical circuitry.
Counterpropagating optical beams and solitons
Authors: Petrović M. , Belić M. , Denz C. , Kivshar Y.S. 
Published: February 2010

AbstractPhysics of counterpropagating optical beams and spatial optical solitons is reviewed, including the formation of stationary states and spatiotemporal instabilities. First, several models describing the evolution and interactions between optical beams and spatial solitons are discussed, that propagate in opposite directions in nonlinear media. It is shown that coherent collisions between counterpropagating beams give rise to an interesting focusing mechanism resulting from the interference between the beams, and that interactions between such beams are insensitive to the relative phase between them. Second, recent experimental observations of the counterpropagation effects and instabilities in waveguides and bulk geometries, as well as in one‐ and two‐dimensional photonic lattices are discussed. A variety of different generalizations of this concept are summarized, including the counterpropagating beams of complex structures, such as multipole beams and optical vortices, as well as the beams in different media, such as photorefractive materials and liquid crystals.
Hybrid alginate beads with thermal‐responsive gates for smart drug delivery
Authors: Shi Jun , Liu Xiaopei , Sun Ximeng , Cao Shaokui 
Published: February 2010

AbstractPolysaccharide‐based thermo‐responsive material was prepared by grafting PNIPAAm onto hybrid alginate beads, in which a biomineralized polyelectrolyte layer was constructed aiming to enhance the mechanical strength and ensure higher graft efficiency. XPS results demonstrated that the incorporation of PNIPAAm to the hybrid beads was successful, and the PNIPAAm‐grafted beads were more hydrophilic than the ungrafted ones as indicated by their swelling behavior. The drug release behaviors revealed that the grafted beads were both thermo‐ and pH‐sensitive, and the PNIPAAm existed in the pores of the alginate beads acted as the “on–off” gates: the pores of the beads were covered by the stretched PNIPAAm to delay the drug release at 25°C and opened to accelerate the drug release at 37°C because of the shrinking of PNIPAAm molecules. This paper would be a useful example of grafting thermo‐responsive polymers onto biodegradable natural polymer substrate. The obtained beads provide a new mode of behavior for thermo‐responsive “smart” polysaccharide materials, which is highly attractive for targeting drug delivery system and chemical separation. Copyright © 2010 John Wiley & Sons, Ltd.
General exposures to prescription medications by race/ethnicity in a population‐based sample: results from the Boston Area Community Health Survey
Authors: Hall Susan A. , Chiu Gretchen R. , Kaufman David W. , Kelly Judith P. , Link Carol L. , Kupelian Varant , McKinlay John B. 
Published: February 2010

AbstractPurposeFew recent U.S. studies have examined population‐based patterns in prescription drug use and even fewer have considered detailed patterns by race/ethnicity. In a representative community sample, our objectives were to determine the most commonly used prescription drug classes, and to describe their use by age, gender, and race/ethnicity.MethodsCross‐sectional epidemiologic study of 5503 (1767 black, 1877 Hispanic, 1859 white) community‐dwelling participants aged 30–79 in the Boston Area Community Health (BACH) Survey (2002–2005). Using medication information collected from an in‐home interview and medication inventory, the prevalence of use of a therapeutic class (95% confidence interval (95%CI)) in the past month was estimated by gender, age group, and race/ethnicity. Estimates were weighted inversely to the probability of sampling for generalizablity to Boston, MA.ResultsThe therapeutic class containing selective serotonin reuptake inhibitor/serotonin norepinephrine reuptake inhibitor (SSRI/SNRI) antidepressants was most commonly used (14.6%), followed by statins (13.9%), beta‐adrenergic blockers (10.6%), and angiotensin‐converting enzyme (ACE) inhibitors (10.5%). Within all age groups and both genders, black participants were substantially less likely than white to use SSRI/SNRI antidepressants (e.g., black men: 6.0% [95%CI: 3.9–8.1%]; white men: 15.0% [95%CI: 10.2–19.4%]). Other race/ethnic differences were observed: for example, black women were significantly less likely than other groups to use benzodiazepines (e.g., black: 2.6% [95%CI: 1.2–3.9%]; Hispanic: 9.4% [95%CI: 5.8–13.0%]).ConclusionsRace/ethnic differences in use of prescription therapeutic classes were observed in our community sample. Examining therapeutic classes rather than individual drugs resulted in a different distribution of common exposures compared to other surveys. Copyright © 2010 John Wiley & Sons, Ltd.
Flavour physics in the Littlest Higgs Model with T‐parity: effects in the K, Bd/s and D systems
Authors: Recksiegel S. 
Published: February 2010

AbstractThe Littlest Higgs Model with T parity (LHT) is an interesting alternative model for New Physics at the TeV scale. Although Flavour Physics was not the reason for creating the LHT model, significant effects (such as large CP violation where not predicted by the SM) can be created without violating existing experimental bounds. We study the B‐, K‐ and especially the D‐sector.