bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional and nutritional properties of infrared‐ and microwave heat‐moisture‐treated sorghum meals
Authors: Baah Rose Otema , Duodu Kwaku‐Gyebi , Taylor John Reginald Nuttal , Emmambux Mohammad Naushad 
Published: June 2024

AbstractBackground and ObjectivesStarch modification using heat‐moisture treatment (HMT) has been proven to influence starch functionality and nutritional properties as it can increase resistant starch content and reduce the glycemic index (GI). This study aims to determine the nutritional and functional properties of infrared (IR)‐ and microwave (MW) heat‐moisture‐treated (HMT) white and red non‐tannin, and red tannin sorghum meals with the aim of further reducing the estimated GI.FindingsAll treated meals had significantly (p < .05) lower pasting peak viscosity than the untreated samples, possibly due to aggregate formation observed under the light microscope, restricting the starch from swelling to form a high‐viscosity paste. There was a decrease in the percentage of starch hydrolysis between the sorghum types and a further decrease after HMT treatment. A significant difference was observed in the protein digestibility between the sorghum types, but not between the treatments.ConclusionHMT with IR and MW further reduced the starch digestibility, possibly because of changes in the starch molecular configuration.Significance and NoveltyThis study suggests that the changes in the starch functionality and nutritional properties of the HMT‐treated sorghum meals can potentially be useful in the development of lower‐GI sorghum foods. The results also differentiate the characteristics of sorghum types.
Acute onset nitrofurantoin‐induced autoimmune hepatitis after urinary tract infection treatment
Authors: Chaudhary Ammad Javaid , Khan Muhammad Zarrar , Sohail Abdullah , Zaidi Syed Murtaza Haider , Denha Eric , Venkat Deepak 
Published: June 2024

Key Clinical MessageThis case signifies the importance of recognizing DIAIH within the context of antibiotic therapy, especially in older adults and even shortly after common drug exposures for treating UTI.AbstractVarious drugs can induce immune‐mediated liver damage and in rare instances may lead to autoimmune hepatitis. Here we report an 84‐year‐old woman who developed autoimmune hepatitis less than 3 weeks after treatment for urinary tract infection with the antibiotic nitrofurantoin. She presented with jaundice, right upper quadrant abdominal pain, nausea, and vomiting. In the absence of a history of an autoimmune disorder or elevated liver enzymes in the past; elevated liver enzymes after a short course of Nitrofurantoin and the presence of smooth muscle antibodies strongly suggested autoimmune hepatitis, which was confirmed through biopsy sample analysis. The patient scored 7 points on the Naranjo adverse reaction probability scale. The patient's rapid recovery within 1 month of prednisone therapy supports the association of liver damage with nitrofurantoin use.
Catastrophic antiphospholipid syndrome: challenging case and importance of multidisciplinary evaluation and management
Authors: Sorathia Sharmeen , Arockiam Aro Daniela , Haroun Elio , Khurana Rishabh , Hall Alexandra , McCarthy Meghann , Shastri Rupal K. , Sawaf Hanny , McCrae Keith R. , Jellis Christine L. , Wang Tom Kai Ming 
Published: June 2024

Key Clinical MessageOur case depicts a challenging diagnosis of catastrophic antiphospholipid syndrome in a young patient with a heterogenous presentation with extensive clinical course, a wide range of investigations, including multimodality imaging, and multidisciplinary expertise, to initiate prompt treatment addressing multiorgan thrombotic injury.
Primary synovial sarcoma in anal canal: Report of a very rare case
Authors: Saadaat Ramin , Esmat Esmatullah , Abdul‐Ghafar Jamshid , Ullah Saif , Hanifi Ahmed Nasir , Khairy Abdul Latif , Zahier Ahmed Shekib , Haidary Ahmed Maseh , Noor Sarah 
Published: June 2024

Key Clinical MessageExtensive studies are required to understand the behavior as well as prognosis of SS in the colorectal region. IHC staining is essential for the accurate diagnosis when a lesion is encountered at an unusual site.
The coincidence of beta‐thalassemia and hereditary spherocytosis: A case report and literature review
Authors: Habibzadeh Sana , Einakchi Majid , Kalantari Mohammad Ebrahim , Forouhar Farnood , Ma'souminejad Arefeh 
Published: June 2024

Key Clinical MessageWhen a person has both HS and beta‐thalassemia, their clinical symptoms tend to be less severe. This is because these two conditions have contrasting features. If the clinical symptoms and laboratory results cannot be solely attributed to hemolytic anemia, it is important to consider the possibility of another form of hemolytic anemia coexisting.AbstractWe present a 26‐year‐old woman who has been experiencing abdominal pain, jaundice, and anemia for the past 15 years. Initially, she was diagnosed with gallstones and splenomegaly, but after a thorough hematology examination conducted by expert colleagues, it was discovered that she had both beta‐thalassemia and hereditary spherocytosis. The osmotic fragility test confirmed this diagnosis. The patient was advised to undergo both splenectomy and cholecystectomy procedures. It is worth noting that the co‐occurrence of these two conditions is rare.
Dyke‐Davidoff‐Masson syndrome—A rare cause of recurrent seizures in adulthood
Authors: Afrim Patricia , Ofori Emmanuel , Owusu‐Danso Nana Akosua , Gyamfi Rexford Adu , Owusua Denise Akua , Gyabaah Solomon , Sarfo Fred Stephen 
Published: June 2024

Key Clinical MessageIt is important to consider DDMS as a differential diagnosis in any patient with early childhood onset of epilepsy. Early diagnosis and optimal management are key to reducing the disabling effect of DDMS.
Intrarenal epidermoid cyst mimicking a cystic renal cell carcinoma: A case report and review of literature
Authors: Khorraminejad‐Shirazi Mohammadhossein , Nabavizadeh Seyed Ali , Shirazi Yeganeh Babak , Ahmadifar Maryam , Salehipour Mehdi , Jafari Seyed Hamed , Omidifar Navid 
Published: June 2024

Key Clinical MessageThis case highlights the diagnostic pitfalls that can occur when evaluating complex cystic renal masses. Distinguishing epidermoid cysts from renal cell carcinoma is difficult but imperative to guide conservative management when appropriate, avoiding unnecessary nephrectomy.AbstractRenal epidermoid cysts are extremely rare, with only 12 cases reported in the literature. Their radiographic features often resemble cystic renal cell carcinoma, frequently prompting unnecessary nephrectomy. A 64‐year‐old man with a history of nephrolithiasis presented with left flank pain and hematuria. Imaging revealed a complex cystic renal mass suspicious for renal cell carcinoma. Following left radical nephrectomy, histopathology examination revealed a benign epidermoid cyst. Renal presentation of epidermoid cyst poses unique diagnostic and therapeutic challenges. Possible pathogenesis includes ectopic epidermal implantation during embryogenesis or squamous metaplasia following chronic irritation or deficiency. Radiographic distinction from concerning entities like renal cell carcinoma is difficult but imperative to avoid extensive surgery. This case highlights the diagnostic pitfalls and management considerations for renal epidermoid cysts. Additional study of clinical and imaging factors that distinguish epidermoid cysts from renal cell carcinoma can guide conservative management when appropriate, avoiding unnecessary nephrectomy for benign disease.
Ectopic pregnancy with full implantation of the gestational sac in the caesarean section scar managed successfully with laparotomy
Authors: Machairiotis Nikolaos , Stavros Sofoklis , Antonakopoulos Nikolaos , Vrachnis Dionysios , Potiris Anastasios , Fotiou Alexandros , Loukas Nikolaos , Drakakis Peter , Vrachnis Nikolaos 
Published: June 2024

Key Clinical MessageCaesarean scar pregnancy represents one of the rarest locations of ectopic pregnancies. It occurs when the blastocyst is implanted in a scar from a previous caesarean section. A dramatic increase of its prevalence has been observed for the last decades, reaching about 21% globally. Early diagnosis and treatment are crucial to avoid maternal morbidity and mortality. Our case presents the characteristic appearance of a caesarean scar pregnancy with full implantation of the gestational sac in the scar, which was managed successfully with laparotomy.
Native State Stabilization of Amyloidogenic Proteins by Kinetic Stabilizers: Inhibition of Protein Aggregation and Clinical Relevance
Authors: Priyanka  , Raymandal Bitta , Mondal Santanu 
Published: June 2024

AbstractProteinopathies or amyloidoses are a group of life‐threatening disorders that result from misfolding of proteins and aggregation into toxic insoluble amyloid aggregates. Amyloid aggregates have low clearance from the body due to the insoluble nature, leading to their deposition in various organs and consequent organ dysfunction. While amyloid deposition in the central nervous system leads to neurodegenerative diseases that mostly cause dementia and difficulty in movement, several other organs, including heart, liver and kidney are also affected by systemic amyloidoses. Regardless of the site of amyloid deposition, misfolding and structural alteration of the precursor proteins play the central role in amyloid formation. Kinetic stabilizers are an emerging class of drugs, which act like pharmacological chaperones to stabilize the native state structure of amyloidogenic proteins and to increase the activation energy barrier that is required for adopting a misfolded structure or conformation, ultimately leading to the inhibition of protein aggregation. In this review, we discuss the kinetic stabilizers that stabilize the native quaternary structure of transthyretin, immunoglobulin light chain and superoxide dismutase 1 that cause transthyretin amyloidoses, light chain amyloidosis and familial amyotrophic lateral sclerosis, respectively.
A Platform for Medium‐Throughput Cell‐Free Analyses of Microtubule‐Interacting Proteins Using Mammalian Cell Lysates
Authors: Jijumon A. S. , Krishnan Arya , Janke Carsten 
Published: June 2024

AbstractThe microtubule (MT) cytoskeleton performs a variety of functions in cell division, cell architecture, neuronal differentiation, and ciliary beating. These functions are controlled by proteins that directly interact with MTs, commonly referred to as microtubule‐associated proteins (MAPs). Out of the many proteins reported interact with MTs, only a some have been biochemically and functionally characterized so far. One of the limitations of classical in vitro assays and single‐MT reconstitution approaches is that they are typically performed with purified proteins. As purification of proteins can be difficult and time‐consuming, many previous studies have only focused on a few proteins, while systematic analyses of many different proteins by in vitro reconstitution assays were not possible. Here we present a detailed protocol using lysates of mammalian cells instead of purified proteins that overcomes this limitation. Those lysates contain all molecular components required for in vitro MT reconstitution including the endogenous tubulin and the recombinant MAPs, which form MT assemblies upon the injection of the lysates into a microscopy chamber. This allows to directly observe the dynamic behavior of growing MTs, as well as the fluorescently labeled associated proteins by total internal reflection fluorescence (TIRF) microscopy. Strikingly, all proteins tested so far were functional in our approach, thus providing the possibility to test virtually any protein of interest. This also opens the possibility to screen the impact of patient mutations on the MT binding behavior of MAPs in a medium‐throughput manner. In addition, the lysate approach can easily be adapted to other applications that have predominantly been performed with purified proteins so far, such as investigating other cytoskeletal systems and cytoskeletal crosstalk, or to study structures of MAPs bound to MTs by cryo‐electron microscopy. Our approach is thus a versatile, expandable, and easy‐to‐use method to characterize the impact of a broad spectrum of proteins on cytoskeletal behavior and function. © 2024 The Authors. Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Preparation of lysates of human cells for TIRF reconstitution assaysBasic Protocol 2: Quantification of GFP‐tagged MAP concentration in cell lysatesSupport Protocol 1: Purification of KIF5B(N555/T92A) (dead kinesin) protein for TIRF reconstitution assaysSupport Protocol 2: Preparation of GMPCPP MT seeds for TIRF reconstitution assaysBasic Protocol 3: TIRF‐based MT‐MAP reconstitution assays using cell lysates
Comparison of 5G‐IoT antenna performance by using four distinct dielectric materials to verify their efficiencies
Authors: Mukherjee Kaushal , Roy Sahadev 
Published: June 2024

SummaryThe characterization of four different materials, Taconic (D1), FR4 (D2), Lab material (D3), and Created material (D4) using the ring resonator technique, is presented in this study along with a comparison of antenna performance using standard and measured dielectric constant (
) and loss tangent (
) values. This article discussed the comparison of antenna performances on four distinct dielectric materials to verify their efficiencies. Standard values of
for the reported materials are 2.20, 4.40, 2.33, and 10.45 for D1, D2, D3, and D4, respectively, with corresponding
values of 0.0009, 0.02, 0.0012, and 0.00032. The
and
for the materials D1, D2, D3, and D4 are calculated as 2.10, 4.56, 2.51, and 9.89 and 0.00094, 0.01927, 0.0013, and 0.0003025, respectively, using a ring resonator. The materials are utilized as the substrate for a printed single layer Y‐Shape 5G‐IoT antenna in order to validate the measured results. According to the findings of this study, the measurement of
using the resonator approach exhibited errors of 4.54%, 3.63%, 7.82%, and 5.33%, respectively, while the
has errors of 04.44%, 03.65%, 08.33%, and 5.47% for materials D1, D2, D3, and D4, respectively. The outcomes of this work include the numerical calculation of the
and
of four materials and the validation of the measured values using a Y‐Shape 5G‐IoT antenna in the millimeter‐wave 5G frequency bands. Additionally, the values calculated mathematically are compared with the values obtained from the conventional ring resonator.
Downregulation of HIGD1B induces mitochondria‐mediated apoptosis in gastric cancer cells by inactivating Akt and ERK pathways
Authors: Chen Xiangyu , Sun Binghua , Li Shuai 
Published: June 2024

AbstractGastric cancer (GC) is one of the most common malignancies worldwide. Hypoxia‐inducible domain (HIGD) family members (e.g., HIGD1A) have been linked to tumor progression. However, the role of HIGD1B (another HIGD family member) in GC has yet to be fully understood. Based on data from TCGA_GC, GSE65801, and GSE65801 data sets, HIGD1B levels were evaluated in normal and GC tissues. Next, HIGD1B levels were validated by reverse transcription‐quantitative PCR and western blot analysis analyses. Meanwhile, patients with GC in the TCGA_GC cohort were grouped into high‐ and low‐HIGD1B level groups, and overall survival, functional enrichment, and immune infiltration were analyzed. Additionally, gain‐ and loss‐of‐function experiments were performed to determine the function of HIGD1B in GC cells. Compared to normal controls, HIGD1B mRNA levels were significantly elevated in GC tissues. Moreover, high HIGD1B levels may be an independent indicator of poor prognosis in patients with GC. Additionally, high HIGD1B levels were correlated with high stromal and ESTIMATE scores and elevated expression of immune checkpoints in patients with GC. Functional analyses showed that HIGD1B deficiency notably suppressed GC cell proliferation, migration, and invasion. Moreover, HIGD1B deficiency significantly induced mitochondria‐mediated apoptosis in GC cells by inactivating Akt and ERK pathways. Collectively, HIGD1B may predict the prognosis of patients with GC and may function as an oncogene in GC. These findings suggest that HIGD1B may serve as a prognostic biomarker and potential therapeutic target in GC.
Constructing Alkyl Chain Modified FeII Spin Crossover Complexes through Complementary Pair Strategy
Authors: Peng Ya‐Lin , Lu Han‐Han , Shang Meng‐Jia , Sun Hui‐Ying , Jiang Wen‐Jing , Xiao Jing‐Yi , Liu Tao , Meng Yin‐Shan 
Published: June 2024

AbstractFour alkyl chains and cyclohexane modified FeII complexes [Fe(Ln)(L’)](ClO4)2⋅solv (n=1, 2, 3, 4) were synthesized and characterized. X‐ray diffraction study showed that these complexes were constituted by asymmetric mononuclear FeII entities, incorporating alkyl‐chain‐modified bppCOOH (2,6‐bis(1H‐pyrazol‐1‐yl)isonicotinic acid) ligands and 6,6’’‐2,6‐dimethoxyphenyl‐substituted terpy ligand (L’). Magnetic susceptibility measurements revealed that complexes 1, 2, and 3 were in the high spin state across the measured temperature range, whereas complex 4 displayed an incomplete spin crossover phenomenon, with a transition temperature (T1/2) at 240 K. Investigations into variable‐temperature structure and magneto‐structural correlations revealed that the integration of alkyl‐chain‐modified bipyridyl units fostered the π⋅⋅⋅π intra‐ and intermolecular interactions, contributing to distinct crystal stacking and spatial configurations in complex 4 when compared to its counterparts. These findings underscore the pivotal influence of intramolecular interactions on the FeII spin states and highlight the significance of designing flexible ligands for modulating spin‐crossover characteristics.
Stability of color and biologically active compounds of pasteurized juices from potatoes with colored flesh
Authors: Tkaczyńska Agnieszka , Rytel Elżbieta , Kucharska Alicja Z. , Kolniak‐Ostek Joanna , Sokół‐Łętwska Anna 
Published: June 2024

AbstractJuices from potato varieties with colored flesh contain a large amount of biologically active compounds, but they tend to darken enzymatically, which deteriorates the quality. One of the factors that can improve the color of juices is pasteurization. The aim of the study was to investigate the effect of pasteurization temperature on the anthocyanin content and color of juices from potatoes with colored flesh. The research material included juices from potato varieties with red and purple flesh. Juices pasteurized at 75 °C were characterized by the lightest color and an increase in the a* (red color) and b* (yellow color) parameters compared to unpasteurized juices. Pasteurization of juices reduced the amount of glycoalkaloids by an average of 54% compared to unpasteurized juices (larger losses in the content of α‐chaconine than α‐solanine). Purple potato juices showed a higher content of total polyphenols by an average of 30% and anthocyanins by 70% than juices from red potatoes. Pelargonidin and its derivatives were identified in red potato juices, while petunidin and peonidin were the most abundant in purple potato juices. Higher losses of total polyphenols were found in juices from red varieties of potatoes, while anthocyanins were less thermostable in juices from varieties with purple flesh.
Humanitarian crisis amid the military coup in Niger republic; what went wrong?
Authors: Oduoye Malik Olatunde , Ubechu Samuel Chinonso , Zafar Habiba , Noor Zainab , Oyeleke Usman Abolaji , Agbeyewo Ganiyat Temitope 
Published: June 2024

AbstractIntroductionA humanitarian crisis means any event or series of events that are harmful to the health or security of the general population. The Niger Republic is currently facing a serious crisis amid the military coup of July 26, 2023. We fear that if nothing is done about it quickly, there could be a civil war in Niger and other neighboring countries.AimThis present article aims to provide comprehensive knowledge about the ongoing humanitarian crisis in Niger, highlighting the factors that caused it and providing possible solutions for them.MethodologyA literature search was conducted on the recent humanitarian crisis caused by the military coup in Niger Republic using databases such as PubMed, ResearchGate, Google Scholar, World Health Organization, United Nations, online newspapers, and reports from 2013 to 2023.ResultWe found that the humanitarian crisis amid the military coup in Niger Republic has further amplified the vulnerability of millions of Nigeriens to more problems in the Niger Republic. The number of Nigeriens in need of humanitarian assistance has reportedly increased by 65%, from 2.3 million in 2019 to 3.8 million in 2021. More than two million children in Niger have been impacted by the crisis in the country and need humanitarian assistance. Furthermore, other causes of the humanitarian crisis in the Niger Republic include sociodemographic factors, climate change, and other natural disasters.ConclusionThe humanitarian crisis in Niger, caused by different factors such as socioeconomic stagnation, environmental causes, food insecurity, terrorist activities, and internal displacement, is an alarming situation that requires an urgent solution and a response from the international community. There is a need to analyze all the underlying causes of humanitarian crises and find long‐lasting solutions for them.
Effect of Nb‐doping on the MnFeOx catalyst for NH3‐SCR reaction at low‐temperature
Authors: Pei Zhenzhao , Zhao Haiyang , Wang Haipeng , Fu Zhuyue , Yang Kanghua , Mao Kang , Liu Yan 
Published: June 2024

AbstractBACKGROUNDManganese‐based catalysts were considered the most promising catalysts for low‐temperature selective catalytic reduction (NH3‐SCR) reactions. In this work, a series of Mn3Fe2NbyOx catalysts were synthesized by co‐precipitation method for low‐temperature NH3‐SCR and SO2 tolerance tests.RESULTSThe catalysts were characterized by BET, SEM, XRD, XPS, H2‐TPR, and NH3‐TPD. Mn3Fe2Nb0.01Ox exhibited exceptionally high activity with a conversion of almost 100% from 80°C to 240°C, and a NO conversion of more than 75% at 40°C, while the NO conversion at 140°C in the presence of 100 ppm SO2 remained above 90% for 4 h.CONCLUSIONAccording to the characterization results, Nb modification can expand the specific surface area of the catalyst, improve the structure of the catalyst, and make the active components on the catalyst surface more dispersed. Moreover, the Nb‐modified catalyst reduced the content of Mn2+ on the catalyst surface and strengthened the electronic interactions between elements. The synergistic effects among Mn, Fe, and Nb facilitate the formation of more chemically adsorbed oxygen, which enhances the reduction capacity of the catalyst. This facilitates the adsorption of NH3 by the catalyst at low temperatures and promotes the process of low‐temperature NH3‐SCR reaction. More importantly, the Nb‐modified catalyst possessed more weak acid sites, and the higher surface acidity was conducive to the inhibition of SO3 generation, which enhanced the sulfur poisoning resistance of the catalyst. © 2024 Society of Chemical Industry (SCI).