bluuebunny/crossref_metadata_2025_split huggingface dataset:
A methodological approach for data standardization and management of Open Data portals for scientific research groups: a case study on mobile and ubiquitous ecosystems
Authors: Villarreal Vladimir , Muñoz Lilia , González Joseph , Fontecha Jesús , Dobrescu Cosmin C. , Nielsen Mel , Concepción Dimas , Rodriguez Marco 
Published: June 2024

IntroductionIn the scientific research domain, the Open Science movement stands as a fundamental pillar for advancing knowledge and innovation globally. This article presents the design and implementation of the GITCE Open Data Ecosystem (GITCE-ODE) Research Data Management System (RDMS), developed by the Research Group on Emerging Computational Technologies (GITCE) at the Technological University of Panama, as a platform for the long-term storage, publication, and dissemination of research products.MethodsThe architecture of the GITCE-ODE RDMS encompasses the entire data engineering lifecycle, facilitating information processing stages such as extraction, transformation, loading (ETL), as well as the management and analysis of diverse datasets and metadata.ResultsCompliance with the FAIR principles ensures that published data and products are Findable, Accessible, Interoperable, and Reusable, promoting automation in the discovery and reuse of digital resources. Key considerations of the web portal include file format standardization, data categorization, treatment of semantic context, and organization of resources to ensure efficient management and administration of open research data.DiscussionThrough this platform, GITCE aims to foster collaboration, transparency, and accessibility in scientific research, contributing to the ongoing advancement of knowledge transfer and innovation.
Genetic Alchemy unveiled: MicroRNA-mediated gene therapy as the Artisan craft in the battlefront against hepatocellular carcinoma—a comprehensive chronicle of strategies and innovations
Authors: Murshed Abduh , Alnoud Mohammed A. H. , Ahmad Saleem , Khan Safir Ullah , Alissa Mohammed , Alsuwat Meshari A. , Ahmed Ahmed Ezzat , Khan Munir Ullah 
Published: June 2024

Investigating therapeutic miRNAs is a rewarding endeavour for pharmaceutical companies. Since its discovery in 1993, our understanding of miRNA biology has advanced significantly. Numerous studies have emphasised the disruption of miRNA expression in various diseases, making them appealing candidates for innovative therapeutic approaches. Hepatocellular carcinoma (HCC) is a significant malignancy that poses a severe threat to human health, accounting for approximately 70%–85% of all malignant tumours. Currently, the efficacy of several HCC therapies is limited. Alterations in various biomacromolecules during HCC progression and their underlying mechanisms provide a basis for the investigation of novel and effective therapeutic approaches. MicroRNAs, also known as miRNAs, have been identified in the last 20 years and significantly impact gene expression and protein translation. This atypical expression pattern is strongly associated with the onset and progression of various malignancies. Gene therapy, a novel form of biological therapy, is a prominent research area. Therefore, miRNAs have been used in the investigation of tumour gene therapy. This review examines the mechanisms of action of miRNAs, explores the correlation between miRNAs and HCC, and investigates the use of miRNAs in HCC gene therapy.
Geochemical and mineralogical heterogeneity of the Cantung mine tailings: implications for remediation and reprocessing
Authors: Surrette A. , Dobosz A. , Lambiv Dzemua G. , Falck H. , Jamieson H. E. 
Published: June 2024

Reprocessing tailings to recover minerals of economic interest and environmental concern can add value to a project and decrease environmental risk, but dealing with heterogeneity within tailings facilities is a challenge. This study investigates the heterogeneity of the Cantung Mine tailings to assess the potential for reprocessing for both value recovery and remediation purposes. The Cantung Mine, Northwest Territories, was a world-class tungsten (W) deposit that was mined periodically from 1962 to 2015. Geochemical analysis of 196 tailings samples shows substantial heterogeneity in the elements of value (tungsten and copper (Cu)) and elements of environmental concern for acid rock drainage (iron (Fe) and sulfur (S)). Tungsten and copper concentrations range from 0.06 to 1.06 wt% W (average 0.32 wt% W) and 0.05 to 0.48 wt% Cu (average 0.23 wt% Cu). Iron and sulfur concentrations range from 8.25 to 34.08 wt% Fe (average 17.14 wt% Fe) and 2.20 to 19.70 wt% S (average 6.7 wt% S). Characterization of 29 samples by scanning electron microscope with automated mineralogy software shows that geochemical heterogeneity corresponds to mineralogical heterogeneity with variability in the concentrations of scheelite (CaWO4), chalcopyrite (CuFeS2) and pyrrhotite (Fe(1-x)S). Liberation analyses indicate that additional grinding would be necessary to recover scheelite, chalcopyrite or pyrrhotite. Pyrrhotite with monoclinic and hexagonal-orthorhombic forms were identified. Overall, the Cantung tailings display considerable heterogeneity, which could lead to difficulties in reprocessing for economic or environmental benefit, but characterizing the heterogeneity allows for systems to be optimized.
Diverse nitrogen enrichments enhance photosynthetic resistance of Sargassum horneri to ultraviolet radiation
Authors: Li Baoqi , Xu Zhiguang , Jiang Xiaotong , Wu Hongyan , Bao Menglin , Zang Shasha , Yan Fang , Yuan Tingzhu 
Published: June 2024

In recent years, golden tides caused by floating Sargassum have induced severe ecological disasters globally. Eutrophication is a significant factor contributing to the massive spread of Sargassum golden tides. Furthermore, the thalli of Sargassum that float on the ocean surface are subjected to more ultraviolet radiation (UVR). The coupled impact of eutrophication and UVR on the photosynthetic physiology of golden tide species remains unclear. In this study, the thalli of Sargassum horneri, known to cause golden tide, were cultured and acclimated to three distinct nitrogen (N) conditions (natural seawater, NSW; NH4+-N enrichment; and NO3–N enrichment) for 6 days. Subsequently, the thalli were exposed to two different radiation treatments (photosynthetically active radiation (150 W m-2), PAR, 400–700 nm; PAR (150 W m-2) + UVR (28 W m-2), 280–700 nm) for 120 min, to investigate the photosynthetic effects of UVR and N on this alga. The findings demonstrated that exposure to UVR impeded the photosynthetic capacity of S. horneri, as evidenced by a decrease in the maximum photochemical quantum yield (Fv/Fm), photosynthetic efficiency (α) and chlorophyll content. Under diverse N-enrichment conditions, the alga tended to adopt various strategies to mitigate the adverse effects of UVR. NH4+-enrichment dissipated excess UVR energy through a greater increase in non-photochemical quenching (NPQ). While NO3–enrichment protected alga by enhancing N assimilation (higher nitrate reductase activity (NRA) and soluble protein content), and maintained a stable energy captured per unit reaction center for electron transfer (ET0/RC) and a higher net photosynthetic rate. Although different N enrichments could not completely offset the damage caused by UV radiation, they secured the photoprotective ability of S. horneri in several ways.
Design and pilot test of an implicit bias mitigation curriculum for clinicians
Authors: Svetkey Laura P. , Bennett Gary G. , Reese Benjamin , Corsino Leonor , Pinheiro Sandro O. , Fischer Jonathan E. , Seidenstein Judy , Olsen Maren K. , Brown Tyson , Ezem Natalie , Liu Evan , Majors Alesha , Steinhauser Karen E. , Sullivan Brandy H. , van Ryn Michelle , Wilson Sarah M. , Yang Hongqiu , Johnson Kimberly S. 
Published: June 2024

IntroductionClinician implicit racial bias (IB) may lead to lower quality care and adverse health outcomes for Black patients. Educational efforts to train clinicians to mitigate IB vary widely and have insufficient evidence of impact. We developed and pilot-tested an evidence-based clinician IB curriculum, “REACHing Equity.”MethodsTo assess acceptability and feasibility, we conducted an uncontrolled one-arm pilot trial with post-intervention assessments. REACHing Equity is designed for clinicians to: (1) acquire knowledge about IB and its impact on healthcare, (2) increase awareness of one's own capacity for IB, and (3) develop skills to mitigate IB in the clinical encounter. We delivered REACHing Equity virtually in three facilitated, interactive sessions over 7–9 weeks. Participants were health care providers who completed baseline and end-of-study evaluation surveys.ResultsOf approximately 1,592 clinicians invited, 37 participated, of whom 29 self-identified as women and 24 as non-Hispanic White. Attendance averaged 90% per session; 78% attended all 3 sessions. Response rate for evaluation surveys was 67%. Most respondents agreed or strongly agreed that the curriculum objectives were met, and that REACHing Equity equipped them to mitigate the impact of implicit bias in clinical care. Participants consistently reported higher self-efficacy for mitigating IB after compared to before completing the curriculum.ConclusionsDespite apparent barriers to clinician participation, we demonstrated feasibility and acceptability of the REACHing Equity intervention. Further research is needed to develop objective measures of uptake and clinician skill, test the impact of REACHing Equity on clinically relevant outcomes, and refine the curriculum for uptake and dissemination.ClinicalTrials.gov ID: NCT03415308.
Brain and serum lipidomic profiles implicate Lands cycle acyl chain remodeling association with APOEε4 and mild cognitive impairment
Authors: Mares Jason , Costa Ana Paula , Dartora William J. , Wartchow Krista M. , Lazarian Artur , Bennett David A. , Nuriel Tal , Menon Vilas , McIntire Laura Beth J. 
Published: June 2024

IntroductionAt least one-third of the identified risk alleles from Genome-Wide Association Studies (GWAS) of Alzheimer’s disease (AD) are involved in lipid metabolism, lipid transport, or direct lipid binding. In fact, a common genetic variant (ε4) in a cholesterol and phospholipid transporter, Apolipoprotein E (APOEε4), is the primary genetic risk factor for late-onset AD. In addition to genetic variants, lipidomic studies have reported severe metabolic dysregulation in human autopsy brain tissue, cerebrospinal fluid, blood, and multiple mouse models of AD.MethodsWe aimed to identify an overarching metabolic pathway in lipid metabolism by integrating analyses of lipidomics and transcriptomics from the Religious Order Study and Rush Memory Aging Project (ROSMAP) using differential analysis and network correlation analysis.ResultsCoordinated differences in lipids were found to be dysregulated in association with both mild cognitive impairment (MCI) and APOEε4 carriers. Interestingly, these correlations were weakened when adjusting for education. Indeed, the cognitively non-impaired APOEε4 carriers have higher education levels in the ROSMAP cohort, suggesting that this lipid signature may be associated with a resilience phenotype. Network correlation analysis identified multiple differential lipids within a single module that are substrates and products in the Lands Cycle for acyl chain remodeling. In addition, our analyses identified multiple genes in the Lands Cycle acyl chain remodeling pathway, which were associated with cognitive decline independent of amyloid-β (Aβ) load and tau tangle pathologies.DiscussionOur studies highlight the critical differences in acyl chain remodeling in brain tissue from APOEε4 carriers and individual non-carriers with MCI. A coordinated lipid profile shift in dorsolateral prefrontal cortex from both APOEε4 carriers and MCI suggests differences in lipid metabolism occur early in disease stage and highlights lipid homeostasis as a tractable target for early disease modifying intervention.
A phase 1 proof-of-concept study evaluating safety, tolerability, and biological marker responses with combination therapy of CTLA4-Ig and interleukin-2 in amyotrophic lateral sclerosis
Authors: Thonhoff Jason R. , Beers David R. , Zhao Weihua , Faridar Alireza , Thome Aaron , Wen Shixiang , Zhang Aijun , Wang Jinghong , Appel Stanley H. 
Published: June 2024

ObjectiveTo determine whether a combination therapy with abatacept (CTLA4-Ig) and interleukin-2 (IL-2) is safe and suppresses markers of oxidative stress, inflammation, and degeneration in ALS.MethodsIn this open-label study, four participants with ALS received subcutaneous injections of low dose IL-2 (1 × 106 IU/injection/day) for 5 consecutive days every 2 weeks and one subcutaneous injection of CTLA4-Ig (125 mg/mL/injection) every 2 weeks coinciding with the first IL-2 injection of each treatment cycle. Participants received a total of 24 treatment cycles during the first 48 weeks in this 56-week study. They were closely monitored for treatment-emergent adverse events (TEAEs) and disease progression with the ALSFRS-R. Phenotypic changes within T cell populations and serum biological markers of oxidative stress [4-hydroxynonenal (4-HNE) and oxidized-LDL (ox-LDL)], inflammation (IL-18), and structural neuronal degeneration [neurofilament light chain (Nf-L)] were assessed longitudinally.ResultsCTLA4-Ig/IL-2 therapy was safe and well-tolerated in all four participants over the 56-week study. During the first 24 weeks, the average rate of change in the ALSFRS-R was +0.04 points/month. Over the 48-week treatment period, the average rate of change was −0.13 points/month with one participant improving by 0.9 points/month while the other three participants experienced an average decrease of −0.47 points/month, which is slower than the average − 1.1 points/month prior to initiation of therapy. Treg suppressive function and numbers increased during treatment. Responses in the biological markers during the first 16 weeks coincided with minimal clinical progression. Mean levels of 4-HNE decreased by 30%, ox-LDL decreased by 19%, IL-18 decreased by 23%, and Nf-L remained the same, on average, in all four participants. Oxidized-LDL levels decreased in all four participants, 4-HNE and IL-18 levels decreased in three out of four participants, and Nf-L decreased in two out of four participants.ConclusionThe combination therapy of CTLA4-Ig and IL-2 in ALS is safe and well-tolerated with promising results of clinical efficacy and suppression of biomarkers of oxidative stress, neuroinflammation and neuronal degeneration. In this open-label study, the efficacy as measured by the ALSFRS-R and corresponding biomarkers suggests the therapeutic potential of this treatment and warrants further study in a phase 2 double-blind, placebo-controlled trial.Clinical trial registrationClinicalTrials.gov, NCT06307301.
Validity and agreement between dual-energy X-ray absorptiometry, anthropometry and bioelectrical impedance in the estimation of fat mass in young adults
Authors: Mecherques-Carini Malek , Albaladejo-Saura Mario , Vaquero-Cristóbal Raquel , Baglietto Nicolás , Esparza-Ros Francisco 
Published: June 2024

IntroductionAssessment of fat mass has historically employed various methods like Dual-energy X-ray Absorptiometry (DXA), and bioelectrical impedance (BIA), and anthropometry with its set of formulas. However, doubts persist regarding their validity and interchangeability to evaluate fat mass. This research aimed to determine the validity of anthropometry, and BIA in estimating fat mass Vs DXA, considering the influence of sex and hydration status.MethodsA descriptive, cross-sectional study included 265 young adults (161 males and 104 females), assessed through DXA, BIA in a standing position, and anthropometry. A fat mass estimation formula with DXA, a fat mass estimation formula with BIA and 10 fat mass estimation formulas with anthropometry were calculated.ResultsSignificant differences were found across DXA, BIA and anthropometry in both kilograms and percentages for the overall sample (p&lt;0.001), and when the covariable sex was included (p&lt;0.001), with no significant effect of hydration status (p=0.332-0.527). Bonferroni-adjusted analyses revealed significant differences from DXA with anthropometry and BIA in most cases for the overall sample (p&lt;0.001), as well as when stratified by sex (p&lt;0.001–0.016). Lin’s coefficient indicated poor agreement between most of the formulas and methods both in percentage and kilograms of fat mass (CCC=0.135–0.892). In the Bland-Altman analysis, using the DXA fat mass values as a reference, lack of agreement was found in the general sample (p&lt;0.001-0.007), except for Carter’s formula in kilograms (p=0.136) and percentage (p=0.929) and Forsyth for percentage (p=0.365). When separating the sample by sex, lack of agreement was found in males for all methods when compared with both percentage and kilograms calculated by DXA (p&lt;0.001). In the female sample, all methods and formulas showed lack of agreement (p&lt;0.001–0.020), except for Evans’s in percentage (p=0.058).ConclusionThe formulas for fat mass assessment with anthropometry and BIA may not be valid with respect to the values reported with DXA, with the exception of Carter’s anthropometry formula for general sample and Evans’s anthropometry formula for female sample. BIA could also be an alternative if what is needed is to assess fat mass in women as a group.
Effects of physical activity on depression, anxiety, and stress in college students: the chain-based mediating role of psychological resilience and coping styles
Authors: Liu Ming , Liu Huanju , Qin Zhuzhu , Tao Yining , Ye Wan , Liu Renyang 
Published: June 2024

BackgroundPhysical activity can alleviate negative emotions in college students by enhancing mood and cognitive functions. Yet, the mechanisms underlying these benefits remain unclear.PurposeThis study examines the association between physical activity and negative emotions—specifically, depression, anxiety, and stress—in college students. Additionally, we explore the mediating effects of psychological resilience and coping styles to offer theoretical and practical insights for mitigating students’ negative emotions.MethodsUsing a stratified random sampling approach, a total of 1,380 college students, from five universities in Hangzhou, Nanjing, and Wenzhou, were included in the analysis. The survey instruments included the Physical Activity Rating Scale, Connor-Davidson Resilience Scale, Simple Coping Style Scale, and Depression-Anxiety Stress Scale. The data were statistically analyzed using multivariate methods with IBM SPSS 25.0 and the PROCESS V3.3 plug-in.Results(1) College students engage in a low level of physical activity, with male students participating significantly more than female students (p &lt; 0.001). (2) Physical activity was significantly positively correlated with psychological resilience and positive coping styles (t = 9.126, p &lt; 0.001; t = 23.087, p &lt; 0.001) and overall negative correlated with negative emotions in college students (t = −3.601, p &lt; 0.001). (3) Psychological resilience and positive coping styles were found to play a chain mediating role between physical activity and negative emotions. The mediation effect consists of two paths: physical activity → psychological resilience → negative emotions (effect value: −0.0324), and physical activity → psychological resilience → positive coping → negative emotions (effect value: −0.0099). (4) Female students demonstrated higher levels of positive coping (p &lt; 0.001), while male students exhibited more negative emotions (p &lt; 0.001).ConclusionOur study identifies a significant indirect link, mediated by psychological resilience and positive coping styles, between physical activity and the reduction of negative emotions. Targeted interventions addressing gender differences, such as offering special courses and providing specialized exercise programs and emotional management strategies, can enhance psychological resilience and positive coping mechanisms. Consequently, these measures can alleviate the adverse effects of negative emotions. Our findings have broader implications for both research and practical interventions in promoting mental health among college students.
Risk of major depressive increases with increasing frequency of alcohol drinking: a bidirectional two-sample Mendelian randomization analysis
Authors: Feng Weiyu , Zhang Bing , Duan Pengyu , Bi Yong-hong , Jin Zhehao , Li Xiaoyan , Zhao Xiangcheng , Zuo Kun 
Published: June 2024

IntroductionA growing body of evidence suggests that alcohol use disorders coexist with depression. However, the causal relationship between alcohol consumption and depression remains a topic of controversy.MethodsWe conducted a two-sample two-way Mendelian randomization analysis using genetic variants associated with alcohol use and major depressive disorder from a genome-wide association study.ResultsOur research indicates that drinking alcohol can reduce the risk of major depression (odds ratio: 0.71, 95% confidence interval: 0.54~0.93, p = 0.01), while increasing the frequency of drinking can increase the risk of major depression (odds ratio: 1.09, 95% confidence interval: 1.00~1.18, p = 0.04). Furthermore, our multivariate MR analysis demonstrated that even after accounting for different types of drinking, the promoting effect of drinking frequency on the likelihood of developing major depression still persists (odds ratio: 1.13, 95% confidence interval: 1.04~1.23, p = 0.005). Additionally, mediation analysis using a two-step MR approach revealed that this effect is partially mediated by the adiposity index, with a mediated proportion of 37.5% (95% confidence interval: 0.22 to 0.38).DiscussionIn this study, we found that alcohol consumption can alleviate major depression, while alcohol intake frequency can aggravate it.These findings have important implications for the development of prevention and intervention strategies targeting alcohol-related depression.
A systematic review of collaborative robots for nurses: where are we now, and where is the evidence?
Authors: Babalola Grace Titilayo , Gaston Jenna-Marie , Trombetta Joseph , Tulk Jesso Stephanie 
Published: June 2024

Introduction: Robots present an opportunity to enhance healthcare delivery. Rather than targeting complete automation and nurse replacement, collaborative robots, or “cobots”, might be designed to allow nurses to focus on high-value caregiving. While many institutions are now investing in these platforms, there is little publicly available data on how cobots are being developed, implemented, and evaluated to determine if and how they support nursing practice in the real world.Methods: This systematic review investigates the current state of cobotic technologies designed to assist nurses in hospital settings, their intended applications, and impacts on nurses and patient care. A comprehensive database search identified 28 relevant peer-reviewed articles published since 2018 which involve real studies with robotic platforms in simulated or actual clinical contexts.Results: Few cobots were explicitly designed to reduce nursing workload through administrative or logistical assistance. Most included studies were designed as patient-centered rather than nurse-centered, but included assistance for tasks like medication delivery, vital monitoring, and social interaction. Most applications emerged from India, with limited evidence from the United States despite commercial availability of nurse-assistive cobots. Robots ranged from proof-of-concept to commercially deployed systems.Discussion: This review highlights the need for further published studies on cobotic development and evaluation. A larger body of evidence is needed to recognize current limitations and pragmatic opportunities to assist nurses and patients using state-of-the-art robotics. Human-centered design can assist in discovering the right opportunities for cobotic assistance. Committed research-practice partnerships and human-centered design are needed to guide the technical development of nurse-centered cobotic solutions.