bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of RNase L with a Ras GTPase‐activating‐like protein IQGAP1 in mediating the apoptosis of a human cancer cell‐line
Authors: Sato Akira , Naito Tomoharu , Hiramoto Akiko , Goda Kazato , Omi Takuya , Kitade Yukio , Sasaki Takuma , Matsuda Akira , Fukushima Masakazu , Wataya Yusuke , Kim Hye‐Sook 
Published: August 2010

Mammalian intracellular ribonuclease L (RNase L) is a latent endoribonuclease that functions against viral infections as an apoptosis‐inducing protein, and its activity requires intracellular 5′‐end‐triphosphorylated‐2′,5′ oligoadenylates (2‐5A) as an activator. Previously, we showed that RNase L can be activated in human cancer cell line HT1080 by an RNA polymerase I inhibitor, 1‐(3‐C‐ethynyl‐β‐d‐ribo‐pentofuranosyl)cytosine (3′‐ethynylcytidine; ECyd). In ECyd‐treated cells, knockdown of the RNase L resulted in a marked decrease in c‐jun N‐terminal kinase (JNK) phosphorylation, thereby inhibiting apoptosis. We investigate RNase L binding partners by focused proteomic approach using immunoprecipitation with anti‐RNase L IgG and mass spectrometry. We found that the IQ motif‐containing Ras GTPase‐activating‐like protein 1 (IQGAP1) can associate with RNase L, and that phosphorylation occurs on the IQGAP1. ECyd‐induced JNK phosphorylation and apoptosis were inhibited when IQGAP1 was knocked down with a small interfering RNA. These results raise the interesting possibility that the RNase L–IQGAP1 association may regulate JNK phosphorylation in RNase L‐madiated apoptosis. It is likely IQGAP1 works as a regulator in apoptosis.Structured digital abstract
MINT‐7990574: RNaseL (uniprotkb:Q05823) physically interacts (MI:0914) with Kinesin‐like protein KIF23 (uniprotkb:Q02241) and Ras GTPase‐activating‐like protein IQGAP1 (uniprotkb:P46940) by anti bait coimmunoprecipitation (MI:0006)
MINT‐7990598: RNaseL (uniprotkb:Q05823) physically interacts (MI:0915) with IQGAP1 (uniprotkb:P46940) by anti bait coimmunoprecipitation (MI:0006)
Quality of life and sexual function of women with urinary incontinence
Authors: OZKAN Sevgi , OGCE Filiz , CAKIR Dilek 
Published: August 2010

AbstractAim:  The aim of this study was to examine the urinary incontinence (UI) types on the sexual function and quality of life (QOL) of women with UI and the correlation between sexual function and QOL.Methods:  The sample for this descriptive study was comprised of 122 women who presented to obstetrics and gynecology and urology outpatient clinics at university hospitals in Denizli and Izmir, Turkey, who had UI, who were sexually active, who volunteered to participate in the study, and who were chosen by a convenience sampling method. A sociodemographic data collection form, the Female Sexual Function Index (FSFI), and the Incontinence Quality of Life (I‐QOL) questionnaire were used for data collection in the study.Results:  The mixed type of incontinence had an effect on the women's quality of life, mixed and stress incontinence affected the FSFI's pain subscale, and the total sexual functioning score was lower for the women with mixed incontinence. Even though the correlation values were low, it was clear that there was a positive correlation between sexual function and quality of life.Conclusions:  Among the incontinence types, a significant difference was determined by the FSFI and I‐QOL. Urinary incontinence seems to be the predictor of sexual function and quality of life. As a result, a comprehensive assessment of patients with UI is recommended because this condition has a negative influence on their sexual function and quality of life.
The Impact of Erectile Dysfunction on the Quality of Life of Men Undergoing Hemodialysis and Its Association with Depression
Authors: Fernandes Gisele Vajgel , Dos Santos Rondineli Roberto , Soares Waldênio , De Lima Louize Gomes , De Macêdo Breno Santiago , Da Fonte João Eduardo , De Carvalho Bruno Silva Peixoto , Coelho Sandra Neiva , Calado Adriano Almeida 
Published: August 2010

ABSTRACT

Introduction
Erectile dysfunction (ED) is highly prevalent among men undergoing hemodialysis.

Aim
This study was performed to identify the influence of ED on the patient’s quality of life (QoL) and to evaluate the influence of depression on erectile function of these patients.

Main Outcome Measures
For this multicenter cross-sectional study, 275 patients were interviewed through questionnaires: the five-item version of the International Index of Erectile Function was used for diagnosing and classifying ED; the Medical Outcomes Study Questionnaire 36-Item Short Form Health Survey (SF-36) for scoring QoL; and the Hospital Anxiety and Depression Scale (HADS) to evaluate depressive symptoms. Linear regression was used to examine the associations between some of the variables and ED. Predialytic biochemical and hematological parameters were determined during the longer interdialytic period.

Results
Patients had a mean age of 48.6 ± 12.8 years, and the ED prevalence was 72.3%. Advanced age, diabetes and depression score were independent risk factors for the development of ED as confirmed by linear regression (P &lt;0.001, P = 0.002, and P &lt;0.001, respectively). QoL was worse among patients with any degree of ED, and the scores were statistically significant for overall health rating (P = 0.016), physical composite score (P = 0.003), bodily pain (P = 0.042), physical functioning (P &lt;0.001), and vitality (P = 0.005). Furthermore, more severe forms of ED were associated with a lower QoL. After adjustment for some variables, such as age, time under dialysis, hemoglobin, albumin, parathyroid hormone, Kt/V, and depression, linear regression showed that domains related to poorer physical functioning (P = 0.047) and decreased vitality (P = 0.009) were significantly related to ED.

Conclusion
Depression is an important trigger for the development of ED in hemodialysis patients, and this sexual condition is an independent risk factor for their poor QoL.
Efficacy and Safety of an Orodispersible Vardenafil Formulation for the Treatment of Erectile Dysfunction in Elderly Men and Those with Underlying Conditions: An Integrated Analysis of Two Pivotal Trials
Authors: Sperling Herbert , Gittelman Marc , Norenberg Christiane , Ulbrich Ernst , Ewald Silke 
Published: August 2010

ABSTRACT

Introduction
Men with erectile dysfunction (ED) are typically older and have one or more underlying cardiovascular conditions.

Aim
To determine the efficacy and safety of a new orodispersible tablet (ODT) formulation of vardenafil for the treatment of ED, and whether age, or the presence of underlying conditions affects treatment outcomes.

Methods
This is an integrated analysis of data from two phase III, double-blind, multicenter, randomized, parallel-group, placebo-controlled studies that compared 10 mg on-demand vardenafil ODT with placebo in a general population of men with ED, stratified so that approximately 50% of patients were aged ≥65 years. Results were reported by age (&lt;65 vs. ≥65 years) and presence/absence of diabetes, dyslipidemia, or hypertension.

Main Outcome Measures
Primary measures were the erectile function domain of the International Index of Erectile Function (IIEF-EF) and Sexual Encounter Profile questions 2 (SEP2) and 3 (SEP3).

Results
Of the 701 men randomized (51% aged ≥65 years), 686 were included in the intent-to-treat population (placebo, n = 334; vardenafil ODT, n = 352). Vardenafil ODT was significantly superior to placebo for all primary efficacy measures, regardless of age, baseline ED severity, or underlying condition (P &lt; 0.0001 for vardenafil vs. placebo for each endpoint). IIEF-EF scores and SEP2/3 success rates in older patients and men with underlying conditions were not significantly different to those of younger patients or men without underlying conditions. Adverse events (AEs) were mostly mild to moderate in severity, occurring with higher incidence in the vardenafil vs. placebo group. The most frequently reported drug-related AEs in the vardenafil group were headache, flushing, nasal congestion, dizziness, and dyspepsia, consistent with the known safety profile of phosphodiesterase type 5 inhibitors.

Conclusions
Vardenafil ODT significantly improves erectile function in men with ED regardless of age, baseline ED severity, or underlying condition.
The Female Factor: Predicting Compliance with a Post-Prostatectomy Erectile Preservation Program
Authors: Moskovic Daniel J. , Mohamed Osama , Sathyamoorthy Kumaran , Miles Brian J. , Link Richard E. , Lipshultz Larry I. , Khera Mohit 
Published: August 2010

ABSTRACT

Introduction
Early post-radical prostatectomy (RP) erectile preservation (EP) therapy may be critical to preserve erections after surgery.

Aim
To assess if pre-RP female sexual function predicts of partner compliance with an EP protocol.

Main Outcome Measures
Compliance, defined as use of localized penile EP therapy (intracavernosal injections [ICIs], vacuum erection device [VED], or alprostadil) at 3 and 6 months after RP.

Methods
Records of patients enrolled in our EP program from April 2007 to June 2008 were reviewed. Before surgery, patients completed the Sexual Health Inventory for Men (SHIM) and their female partners completed the Female Sexual Function Index (FSFI) questionnaire. Prior to surgery, patients were advised to take sildenafil 25 mg every nightly and use a 250-µg alprostadil suppository three times/week. At 1 month, additional daily use of a VED was encouraged. All patients unable to achieve erections sufficient for penetration were encouraged to initiate ICI of Trimix (phentolamine, papaverine, and PGE1) twice weekly after 3 months following surgery. Data were analyzed using binary logistic regression analysis holding all input variables constant.

Results
Twenty-nine patients had preoperative SHIM &gt; 7 and pre-RP partner FSFI data available. After a 4-week follow-up, compliance with alprostadil suppository declined and both ICI and VED usage increased. At 6 months, six (25.0%) patients had return of natural erectile function and 22 (91.7%) were achieving assisted erections. Higher preoperative partner FSFI scores were associated with greater compliance to the localized penile therapy component of our EP protocol (risk ratio 3.8, P = 0.05).

Conclusions
Preoperative female sexual function correlated with greater partner compliance with the localized component of our EP protocol. Consideration of a female partner’s preoperative sexual function in predicting patient erectile function recovery after RP is warranted. Future studies are necessary to determine the clinical significance of this factor.
Validation of the Sexual Interest and Desire Inventory-Female in Hypoactive Sexual Desire Disorder
Authors: Clayton Anita H. , Goldmeier David , Nappi Rossella E. , Wunderlich Glen , Lewis-D’Agostino Diane J. , Pyke Robert 
Published: August 2010

ABSTRACT

Introduction
The Sexual Interest and Desire Inventory-Female (SIDI-F) is a 13-item scale developed as a clinician-administered assessment tool to measure hypoactive sexual desire disorder (HSDD) severity in women.

Aim
To estimate the reliability and validity of the SIDI-F as a measure of HSDD severity.

Methods
Women, aged 18–65 years, with primary HSDD, Female Sexual Arousal Disorder (FSAD), or no Female Sexual Dysfunction (no FSD) participated in two nontreatment studies (in North America and Europe). On days 0 and 28, subjects were assessed using the SIDI-F, Female Sexual Function Index (FSFI), Changes in Sexual Functioning Questionnaire-Female (CSFQ-F), Locke–Wallace Marital Adjustment Test (MAT) and the Female Sexual Distress Scale (FSDS).

Main Outcome Measures
Discriminant validity, convergent validity, divergent validity, test–retest validity, and internal consistency of the SIDI-F.

Results
The North American study enrolled women with HSDD (N=113), FSAD (N=49) and no FSD (N=61); the European study enrolled women with HSDD (N=130) and no FSD (N=124). In both studies, mean SIDI-F total score for women with HSDD was lower than for those with no FSD (P &lt;0.001, for all) demonstrating discriminant validity. Further, mean SIDI-F total score for women with HSDD was lower than for those with FSAD in the North American study (P &lt;0.001). Convergent validity with the FSFI and CSFQ-F and divergent validity with MAT were demonstrated. Test–retest reliability and internal consistency were high.

Conclusions
The SIDI-F is a valid and reliable measure of HSDD severity in women.
Research Article: Synthetic Peptide Vaccine Development: Designing Dual Epitopes into a Single Pilin Peptide Immunogen Generates Antibody Cross‐reactivity between Two Strains of Pseudomonas aeruginosa
Authors: Hackbarth Clifton , Hodges Robert S. 
Published: August 2010

One of the main challenges of Pseudomonas aeruginosa vaccine development is the design of an antigen that elicits cross‐reactive antibodies against multiple virulent strains. Using a rational design approach, we have developed a single 17‐residue peptide immunogen that generates antibodies that target the receptor‐binding domain of the type IV pilus of more than one strain of P. aeruginosa. Using the receptor‐binding domain sequence, of native strain PAO as a template, we have systematically changed up to five residues in the PAO sequence of the peptide immunogen into that of the PAK sequence. We show by indirect and competitive ELISA that the mutant peptide immunogens elicit the development of polyclonal sera that is cross‐reactive to both native strain PAO and PAK pilin. We further show that there are at least two separate antibody populations in the polyclonal sera that possess closely related epitopes but which are each strain specific. Moreover, part of the epitope for the PAO‐specific antibodies consists of several residues outside the disulfide loop of the receptor‐binding domain. This allows us to create two unique epitopes within the same receptor‐binding domain sequence.
Renewed Interest in Chlorthalidone: Evidence From the Veterans Health Administration
Authors: Ernst Michael E. , Lund Brian C. 
Published: August 2010

J Clin Hypertens (Greenwich).Thiazide‐type diuretics are recommended in national hypertension treatment guidelines, but these guidelines do not give preference to a specific thiazide. Recently, increased use of chlorthalidone has been advocated. The authors reviewed national outpatient prescription data from the Veterans Health Administration from 2003 to 2008 to describe the prescribing trends within the thiazide class, focusing on hydrochlorothiazide and chlorthalidone. Among total thiazide users, the proportion who received hydrochlorothiazide remained stable (95.6% in 2008), but the number of new users of hydrochlorothiazide decreased nearly 30% during this period. In contrast, the proportion of chlorthalidone use among total thiazide users more than doubled (1.1% in 2003 to 2.4% in 2008), and the number of new chlorthalidone users increased by more than 40%. At the time of initiation, chlorthalidone was more likely to be added to an existing antihypertensive regimen; one quarter (25.1%) of new hydrochlorothiazide starts were in patients not receiving concurrent antihypertensive medications, compared with only 12.1% for chlorthalidone (odds ratio, 0.44; 95% confidence interval, 0.42–0.46). Evaluation of national prescribing trends indicates that hydrochlorothiazide remains the most commonly prescribed thiazide, but there appears to be a shift toward more new users of chlorthalidone. J Clin Hypertens (Greenwich). 2010;12:927–934. © 2010 Wiley Periodicals, Inc.
Non‐surgical palatal expansion in adult patient: a clinical case report
Authors: Pithon M.M. 
Published: August 2010

AbstractAim:  The objective of the present study was to report a case of non‐surgical palatal expansion in an adult patient.Report of case:  A Hyrax‐type expander was mounted onto the six upper teeth of the patient who was instructed to activate the orthodontic appliance twice a day at 12 h intervals.Results:  After 15 days, suture separation was observed, which was confirmed by the presence of midline diastema and widening of the radiolucent image of the suture on occlusal radiograph. Based on this observation, the appliance was further activated for 7 days, thus totalising 21 days of activation and expander screw opening of 10.5 mm.Conclusion:  One can conclude that in specific cases, it is possible to obtain an opening of the midpalatal suture in adult patients, thus avoiding surgical procedures.
Symptoms related to glaucomatous visual field abnormalities among male Japanese workers in a population‐based setting
Authors: Tatemichi Masayuki , Nakano Tadashi , Hayashi Takeshi , Tanaka Katsutoshi , Hiro Hisanori , Miyamoto Toshiaki , Aratake Miho , Nishinoue Norihide , Yamazaki Akira , Nakadate Toshio , Sugita Minoru 
Published: August 2010

Abstract.Purpose:  To identify symptoms potentially related to glaucomatous visual field abnormalities (VFAs) in a population‐based setting, and to assess the applicability of using these symptoms to identify persons at risk of developing glaucoma.Methods:  In this study, 10 214 Japanese male general workers (mean age, 45.3 ± 8.8 years) filled out a self‐administered questionnaire and underwent frequency doubling technology (FDT) perimetry testing. The questionnaire inquired about whether the participant was suffering from any of nine symptoms, with scores for each response graded on a four‐item Likert scale: 0 (none), 1 (rarely), 2 (sometimes), and 3 (always). Results from the questionnaire were compared among three groups: participants without any VFAs (9767), participants with VFAs as determined by the FDT test (FDT‐VFA; 447), and 227 participants (of the 447 FDT‐VFA participants) with glaucomatous VFAs who were newly diagnosed by ophthalmologic examinations.Results:  The mean summed scores for the total items were significantly (p < 0.01) higher in FDT‐ and glaucomatous VFA groups than in normal subjects. In particular, responses citing the symptoms ‘feeling of something in the front of the eye’ and ‘feeling of hardness to see in dark places’ were significantly (p < 0.05) more frequent in subjects with FDT‐ and glaucomatous VFAs than among normal participants. However, the respective areas under the receiver operating characteristic curve of summed scores for the nine total items and for the two items which showed significant differences for the glaucoma groups were 0.57 (95% confidence interval = 0.53–0.60) and 0.58 (95% confidence interval = 0.54–0.61).Conclusion:  Although the symptoms ‘feeling of hardness to see in dark places’ and ‘feeling of something in the front of the eye’ could be associated with glaucomatous VFA in a population‐based setting, inquiring about symptoms is of little aid in identifying subjects with glaucomatous VFA as a strategy for public health.
Animal Health Policy Principles for Highly Pathogenic Avian Influenza: Shared Experience from China and Canada
Authors: Stephen C. , Ninghui L. , Yeh F. , Zhang L. 
Published: August 2010

SummaryAnimal health policy for highly pathogenic avian influenza (HPAI) must, for the time being, be based on expert opinion and shared international experience. We used the intellectual capital and knowledge of experienced Chinese and Canadian practitioners and policy makers to inform policy options for China and find shared policy elements applicable to both countries. No peer‐reviewed comprehensive evaluations or systematic regulatory impact assessments of animal health policies were found. Sixteen guiding policy principles emerged from our thematic analysis of Chinese and Canadian policies. We provide a list of shared policy goals, targets and elements for HPAI preparedness, response and recovery. Policy elements clustered in a manner consistent with core public health competencies. Complex situations like HPAI require complex and adaptive policies, yet policies that cross jurisdictions and are fully integrated across agencies are rare. We encourage countries to develop or deploy capacity to undertake and publish regulatory impact assessments and policy evaluation to identify policy needs and provide a basis for evidence‐based policy development.
Spatially weighted mutual information image registration for image guided radiation therapy
Authors: Park Samuel B. , Rhee Frank C. , Monroe James I. , Sohn Jason W. 
Published: August 2010

Purpose:To develop a new metric for image registration that incorporates the (sub)pixelwise differential importance along spatial location and to demonstrate its application for image guided radiation therapy (IGRT).Methods:It is well known that rigid‐body image registration with mutual information is dependent on the size and location of the image subset on which the alignment analysis is based [the designated region of interest (ROI)]. Therefore, careful review and manual adjustments of the resulting registration are frequently necessary. Although there were some investigations of weighted mutual information (WMI), these efforts could not apply the differential importance to a particular spatial location since WMI only applies the weight to the joint histogram space. The authors developed the spatially weighted mutual information (SWMI) metric by incorporating an adaptable weight function with spatial localization into mutual information. SWMI enables the user to apply the selected transform to medically “important” areas such as tumors and critical structures, so SWMI is neither dominated by, nor neglects the neighboring structures. Since SWMI can be utilized with any weight function form, the authors presented two examples of weight functions for IGRT application: A Gaussian‐shaped weight function (GW) applied to a user‐defined location and a structures‐of‐interest (SOI) based weight function. An image registration example using a synthesized 2D image is presented to illustrate the efficacy of SWMI. The convergence and feasibility of the registration method as applied to clinical imaging is illustrated by fusing a prostate treatment planning CT with a clinical cone beam CT (CBCT) image set acquired for patient alignment. Forty‐one trials are run to test the speed of convergence. The authors also applied SWMI registration using two types of weight functions to two head and neck cases and a prostate case with clinically acquired CBCT/MVCT image sets. The SWMI registration with a Gaussian weight function (SWMI‐GW) was tested between two different imaging modalities: CT and MRI image sets.Results:SWMI‐GW converges 10% faster than registration using mutual information with an ROI. SWMI‐GW as well as SWMI with SOI‐based weight function (SWMI‐SOI) shows better compensation of the target organ's deformation and neighboring critical organs’ deformation. SWMI‐GW was also used to successfully fuse MRI and CT images.Conclusions:Rigid‐body image registration using our SWMI‐GW and SWMI‐SOI as cost functions can achieve better registration results in (a) designated image region(s) as well as faster convergence. With the theoretical foundation established, we believe SWMI could be extended to larger clinical testing.