bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of tissue disorder markers in the evaluation of disease progress and outcome prediction: a prospective cohort study in non‐cardiac critically ill patients
Authors: Ye Jing , Chen Zi , Wang Tingsong , Tong Jianjing , Li Xiaoguang , Jiang Jie , Chen Erzhen , Lu Yiming 
Published: November 2010

AbstractBackground: Acute Physiology and Chronic Health Evaluation (APACHE) has a remarkable association with clinical syndrome, life expectancy, and length of ward stay. But the defects are obvious. It is crucial to detect an effective and convenient evaluation method to monitor disease progress and predict outcome. Objective: To determine whether myoglobin (Mb) and other five tissue disorder biomarkers, troponin‐I, creatine‐kinase, creatine kinase–muscle brain, aspartate aminotransferase, and lactate dehydrogenase are independent predictors of disease progress and mortality in non‐cardiac critical illness. Methods: A prospective study with 179 patients admitted to the Intensive Care Unit was conducted. All serum tissue disorder markers and APACHE‐II score were measured within 24 hr of admission. Results: All the six biomarkers were significantly correlated with disease severity stratified by APACHE‐II and outcome. Serial blood samples were taken from 17 patients on detection of two new organs failure. The occurrence of organs failure was significantly associated with the elevation of Mb, troponin‐I, and APACHE‐II. Multivariate analysis demonstrated that elevated Mb was the principal risk factor related to mortality either during hospitalization or 180‐day followup. Kaplan–Meier method and log‐rank test also showed that patients with elevated Mb levels had significantly shorter survival. The mortality was higher in patients with both Mb>500 ng/ml and APACHE‐II>20 than in those with only Mb>500 ng/ml or APACHE‐II>20. Conclusion: All the six tissue disorder markers are predictors of disease severity, organ failure, and outcome in non‐cardiac critically illness. Among them, Mb plays a pivotable role. The combined use of Mb and APACHE‐II suggest an effective method to determine the outcome of critical ill syndrome. J. Clin. Lab. Anal. 24:376–384, 2010. © 2010 Wiley‐Liss, Inc.
Platelet count and erythrocyte sedimentation rate are good predictors of Kawasaki disease: ROC analysis
Authors: Xiu‐Yu Song , Jia‐Yu Huang , Qiang Hong , Shu‐Hui Dai 
Published: November 2010

AbstractBackground and Aims: Kawasaki disease (KD) is the leading cause of acquired pediatric cardiac disease and requires a timely diagnosis. Available effective therapy is ideally administered within 10 days of illness diagnosis. Recent reports of several laboratory tests in KD have been published. In this study, we aimed to evaluate the sensitivity and specificity of several laboratory tests. Methods: We performed a retrospective study of consecutive patients diagnosed with KD from January to December 2008. We studied the sensitivity and specificity of several different tests [T‐cell subgroups, platelet count, erythrocyte sedimentation rate (ESR), and C‐reactive protein (CRP)] to predict KD using receiveroperator characteristic curve analysis. Results: No significant difference was demonstrated in T‐cell subgroups between patients with KD and referent patients (P>0.05). However, platelet count, ESR, and CRP were significantly higher in patients with KD than in referent patients (P<0.05). ESR showed a sensitivity of 93.9% and specificity of 83.3% with a cut‐off of 15 mm/hr (area under the curve [AUC], 89.1%; P=0.03). Platelet count showed a sensitivity of 70.6% and specificity of 75% with a cut‐off of 336.5×109/l (AUC, 71.2%; P=0.03). Conclusions: These results indicate that platelet count and ESR are good predictors of KD. J. Clin. Lab. Anal. 24:385–388, 2010. © 2010 Wiley‐Liss, Inc.
Ischemia‐modified albumin in acute aortic dissection
Authors: Sbarouni Eftihia , Georgiadou Panagiota , Marathias Aikaterini , Panagiotakos Demosthenes , Geroulanos Stefanos , Voudris Vassilis 
Published: November 2010

AbstractBackground: Acute aortic dissection (AOD) is associated with high mortality and early diagnosis and treatment are essential. Ischemia‐modified albumin (IMA) is a marker of myocardial ischemia whereas cardiac enzymes are released when myocardial necrosis occurs. We investigated, for the first time, whether IMA increases in AOD either at presentation or after surgery. Methods: We studied 46 consecutive patients with documented AOD; we also evaluated 13 consecutive patients with dilated ascending aortas scheduled for elective surgery and admitted for preoperative coronary angiography; 46 age‐matched normal subjects served as controls. Only patients with acute onset of symptoms were included. We evaluated IMA, cardiac enzymes, N‐terminal pro‐B‐type natriureticpeptide, albumin, C‐reactive protein (CRP), and D‐dimers on admission, 24 hr post‐operatively and 4 days post‐operatively. Duration from symptom onset to the first sample was 23±17 hr. Results: IMA did not differ between patients with AOD at presentation (93±19 U/ml), patients with chronic aneurysms (90±14 U/ml) and normal controls (91±9 U/ml). In addition, IMA did not change significantly after surgical repair. IMA, at baseline, however, correlated positively with time from symptom onset as well as CRP levels (P=0.05 and P=0.007, respectively). Conclusion: IMA is not elevated in AOD when blood sampling is performed within 23±17 hr after symptom onset nor increases after surgery. J. Clin. Lab. Anal. 24:399–402, 2010. © 2010 Wiley‐Liss, Inc.
What are the cut‐off levels for IL‐6 and CRP in neonatal sepsis?
Authors: Celik İstemi Han , Demirel Fatma Gamze , Uras Nurdan , Oguz Serife Suna , Erdeve Omer , Biyikli Zeynep , Dilmen Ugur 
Published: November 2010

AbstractNeonatal sepsis, characterized by systemic signs of infection in the first month of life, remains an important clinical syndrome. Despite advances in neonatology, it has high rates of mortality and morbidity. The combine or alone usage of interleukin‐6 (IL‐6) and C‐reactive protein (CRP) has recently been proven to be useful in the early diagnosis of sepsis in newborns. The study included 282 patients; there were 232 in Group I (170 proven and 62 clinical sepsis) and 50 in Group II (control group). The optimum cut‐off value in the diagnosis of neonatal sepsis was found to be 24.65 pg/ml for IL‐6 and 4.82 mg/l for CRP. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value (NPV) of this IL‐6 cut‐off for neonatal sepsis were 72, 84, 95, and 42%, respectively. Sensitivity, specificity, PPV, and NPV of the CRP cut‐off for neonatal sepsis were 67, 97, 99, and 39%, respectively. The combination of IL‐6 (>24.65 pg/ml) and CRP (>4.82 mg/l) in the diagnosis of neonatal sepsis gave sensitivity, specificity, PPV, and NPV of 53, 100, 100, and 33%, respectively. To our knowledge, this is the largest reported study seeking to determine cut‐off levels for IL‐6 and CRP in the diagnosis of neonatal sepsis. In conclusion, we think that it is useful to evaluate IL‐6 and CRP, in combination, for the early diagnosis of neonatal sepsis. J. Clin. Lab. Anal. 24:407–412, 2010. © 2010 Wiley‐Liss, Inc.
Effect of spironolactone, potassium canrenoate, and their common metabolite canrenone on dimension Vista Digoxin Assay
Authors: Dasgupta Amitava , Johnson Myrtle J. 
Published: November 2010

AbstractSpironolactone, a potassium sparing diuretic metabolized to canrenone, is often used with digoxin to treat various conditions including congestive heart failure. Potassium canrenoate is a similar drug that is also metabolized to canrenone. Due to reported interference of spironolactone, potassium canrenoate, and their common metabolite canrenone with digoxin immunoassays, we investigated potential interference of these compounds with Dimension Vista Digoxin immunoassay using Flex reagent cartridge. Aliquots of a drug‐free serum pool were supplemented with various amounts of spironolactone, potassium canrenoate, or canrenone and apparent digoxin values were measured using Dimension Vista digoxin assay, we observed none‐detected value except when aliquots were supplemented with higher amounts of spironolactone or canrenone. Similarly, when aliquots of a serum digoxin pool (prepared by pooling specimens from patients receiving digoxin) where further supplemented with various amounts of spironolactone, potassium canrenoate, or canrenone, we observed moderately falsely elevated digoxin values only in specimens containing higher amounts of spironolactone or canrenone. We conclude that spironolactone and canrenone but not potassium canrenoate may cause modest interference with Dimension Vista digoxin assay but such interferences may not be clinically significant except with very high amounts of canrenone. J. Clin. Lab. Anal. 24:413–417, 2010. © 2010 Wiley‐Liss, Inc.
Is specific gravity a good estimate of urine osmolality?
Authors: Imran Sethi , Eva Goldwater , Christopher Shutty , Flynn Ethan , Henner David 
Published: November 2010

AbstractContext: Urine specific gravity (USG) is often used by clinicians to estimate urine osmolality. USG is measured either by refractometry or by reagent strip. Objective: We studied the correlation of USG obtained by either method with a concurrently obtained osmolality. Design: Using our laboratory's records, we retrospectively gathered data on 504 urine specimens on patients on whom a simultaneously drawn USG and an osmolality were available. Out of these, 253 USG's were measured by automated refractometry and 251 USG's were measured by reagent strip. Urinalysis data on these subjects were used to determine the correlation between USG and osmolality, adjusting for other variables that may impact the relationship. The other variables considered were pH, protein, glucose, ketones, nitrates, bilirubin, urobilinogen, hemoglobin, and leukocyte esterase. The relationships were analyzed by linear regression. Results: This study demonstrated that USG obtained by both reagent strip and refractometry had a correlation of approximately 0.75 with urine osmolality. The variables affecting the correlation included pH, ketones, bilirubin, urobilinogen, glucose, and protein for the reagent strip and ketones, bilirubin, and hemoglobin for the refractometry method. At a pH of 7 and with an USG of 1.010 predicted osmolality is approximately 300 mosm/kg/H2O for either method. For an increase in SG of 0.010, predicted osmolality increases by 182 mosm/kg/H2O for the reagent strip and 203 mosm/kg/H2O for refractometry. Pathological urines had significantly poorer correlation between USG and osmolality than “clean” urines. Conclusion: In pathological urines, direct measurement of urine osmolality should be used. J. Clin. Lab. Anal. 24:426–430, 2010. © 2010 Wiley‐Liss, Inc.
Correction for effect of cold storage on immature platelet fraction
Authors: Osei‐Bimpong Andrew , Saleh Mohammad , Sola‐Visner Martha , Widness John , Veng‐Pedersen Peter 
Published: November 2010

AbstractIt is common practice to store samples and measure the immature platelet fraction (IPF) only when the initial standard complete blood count and the blood smear indicate thrombocytopenia. However, the cold storage introduces an inherent error in the %IPF. A falsely elevated %IPF carries the risk of potentially misleading the clinicians' interpretation of the thrombopoietic activity, thus hindering the clinical utility of this parameter. To address this issue, we determined the IPF% in individual blood samples from 29 adults at 0, 4, 8, 10, 12, 21, and 24 hr after the blood draw. This study demonstrated that the effect of 4°C storage is linear over a 24 hr period and independent of the initial IPF%. These observations have enabled the development of a proposed algorithm for a corrected IPF%, which is equal to the uncorrected IPF% minus 1.34 times the length of time in hours of storage at 4°C. The proposed correction algorithm extends the utility of this clinically important laboratory parameter. However, care should be taken not to extrapolate the algorithm significantly beyond 24 hr storage, because the experimental data only covered this range. J. Clin. Lab. Anal. 24:431–433, 2010. © 2010 Wiley‐Liss, Inc.
Efficient and one‐pot catalytic synthesis of 2‐imidazolines and bis‐imidazolines with p‐toluenesulfonic acid under solvent free conditions
Authors: Nasr‐Esfahani Masoud , Montazerozohori Morteza , Mehrizi Safie 
Published: November 2010

AbstractA practical, efficient, and inexpensive method for the synthesis of 2‐imidazoline from the reaction of nitriles with ethylenediamine or 1,2‐propanediamine using p‐toluenesulfonic acid or pyridinium p‐toluenesulfonate under reflux conditions is reported. This catalyst can be successfully applied for the chemoselective conversion of dicyanobenzenes to corresponding mono‐ and bis‐imidazolines. The applications of these catalysts are feasible because of easy preparation, easy handling, stability, inexpensive, good activity, and eco‐friendly. J. Heterocyclic Chem., (2011).
Synthesis of 2,3‐diaryl‐3,4‐dihydro‐2H‐1,3‐benzoxazines and their fungicidal activities
Authors: Tang Zilong , Chen Weiwen , Zhu Zhonghua , Liu Hanwen 
Published: November 2010

AbstractA series of novel 2,3‐diaryl‐3,4‐dihydro‐2H‐1,3‐benzoxazines have been prepared in high yields from o‐arylaminomethylphenols and aromatic aldehydes in the presence of SnCl4 for the first time, and their fungicidal activities were investigated too. Some of the products showed good fungicidal activities against Rhizoctonia solani justified by 100% activity of compound 1b. J. Heterocyclic Chem., (2010)
Synthesis of novel 2‐(2‐methylsulfonyl‐1‐methyl‐1H‐imidazol‐5‐yl)‐5‐(alkylsulfonyl)‐1,3,4‐thiadiazoles
Authors: Javidnia Azita , Akbarzadeh Tahmineh , Firoozpour Loghman , Khoobi Mehdi , Shafiee Abbas , Foroumadi Alireza 
Published: November 2010

AbstractStarting from 5‐hydroxymethyl‐2‐mercapto‐1‐methyl‐1H‐imidazole (1), a series of 2‐(1‐methyl‐2‐methylsulfonyl‐1H‐imidazol‐5‐yl)‐5‐alkylthio and 5‐alkylsulfonyl‐1,3,4‐thiadiazole derivatives (9a, 9b, 9c, 9d and 10a, 10b, 10c, 10d) were prepared as potential antimicrobial agents. The structure of the obtained compounds was confirmed by NMR, IR, Mass spectroscopy, and elemental analysis. J. Heterocyclic Chem., (2010)
Determination of uric acid in human urine by capillary zone electrophoresis with indirect laser‐induced fluorescence detection
Authors: Yang Jiajia , Hu Menglin , Cai Yuanli , Tang Jing , Li Hui 
Published: November 2010

AbstractA CZE with indirect LIF detection method was used for the determination of uric acid (UA) in human urine. UA and its coexisting analytes (i.e. hypoxanthine, xanthine and ascorbic acid) could be well separated within 4.5 min at a voltage of 25 kV with 25°C cartridge temperature in a running buffer composed of 5 mM sodium borate, 10% methanol (v/v) and 0.3 μM fluorescein sodium (apparent pH of the final mixed hydro‐organic solution of sodium borate, methanol and fluorescein is 9.5). Under the optimum condition, the method has good linearity relationships (correlation coefficients: 0.9973–0.9987) with ranges of 25–500, 25–350, 25–250 and 25–300 μg/mL for hypoxanthine, ascorbic acid, xanthine and UA, respectively. The detection limits for the analytes were in the range of 0.29–0.90 μg/mL. The intra‐day RSD values for migration times and peak areas were less than 0.43 and 3.27%, respectively. This method was applied to the quantitation of UA in human urine with recoveries in the range of 93.1–107.3%.
Selectivity in separation using π electron‐rich stationary phases for the comprehensive two‐dimensional analysis of café espresso
Authors: Stevenson Paul G. , Mnatsakanyan Mariam , Dennis Gary R. , Shalliker R. Andrew 
Published: November 2010

AbstractThe multidimensional high‐performance liquid chromatography separations of the complex sample matrix found in café espresso coffee were completed on the propyl phenyl and butyl phenyl columns that contain 3 and 4 carbon atoms in the spacer chain, respectively. Phenyl type stationary phases are able to undergo unique π–π interactions with aromatic compounds. Previous works have found that there are differences in retention characteristics between these chain lengths and this was explored further here. It was found that when analysing the separations by quadrants, using a geometric approach to factor analysis and by measuring the normalised mean radius, subtle differences in the separations were observed and the butyl phenyl phase was more selective for the high to medium polarity species. However, there was very little difference in separation behaviour for the hydrophobic components within the coffee sample. Overall, the analysis of the entire separation showed very little difference.
Diaminotrehalose‐capped β‐cyclodextrin, a new member of hemispherodextrins: Synthesis, thermodynamic and spectroscopic characterization and its exploitation in chiral electrokinetic chromatography
Authors: Cucinotta Vincenzo , Giuffrida Alessandro , Grasso Giulia , Maccarrone Giuseppe , Mazzaglia Antonino , Messina Marianna , Vecchio Graziella 
Published: November 2010

AbstractThe diaminotrehalose‐capped derivative of β‐CD, a new member of hemispherodextrins, was synthesized and characterized by NMR spectroscopy. Its protonation constants were determined by potentiometry, and the inclusion of both the enantiomers of dansyl‐phenylalanine was investigated by NMR spectroscopy. Its stereoselective properties were exploited in electrokinetic chromatography by separating four enantiomeric pairs of dansyl derivatives of amino acids.
New approaches of LC‐MS compatible method development on α1‐acid glycoprotein‐based stationary phase for resolution of enantiomers by HPLC
Authors: Michishita Takashi , Franco Pilar , Zhang Tong 
Published: November 2010

AbstractProtein‐based chiral stationary phases, in particular α1‐acid glycoprotein, are chromatographic materials with a very broad application range. The chiral recognition ability of this selector allows the resolution of more than 90% of the molecules tested. A step‐by‐step description of the screening approach that we have developed is described in this article with two objectives in mind: obtaining a high success rate with a straightforward methodology that can be generally applied and allowing LC‐MS compatibility. Second, the screening strategy is required to take into account the main functional nature of the racemic compound to be resolved (neutral, acidic or basic) for method optimisation step, but should be independent of other structural features, in order to allow the routine application of screening sequences and the application to the largest number of samples.
Resource loss, resource gain, and mental health among survivors of Hurricane Katrina
Authors: Zwiebach Liza , Rhodes Jean , Roemer Lizabeth 
Published: November 2010

AbstractPrior research has shown that losses of personal, social, and material resources resulting from traumatic events significantly contribute to psychopathology. Gains of such resources have been shown to have protective effects on posttrauma mental health. Few previous studies of resource change, however, have controlled for pretrauma mental health. The current study, which included 402 survivors of Hurricane Katrina, made use of data collected prehurricane to examine patterns of loss and gain and subsequent mental health. The loss of social support, physical health, and personal property were shown to significantly affect posthurricane psychological distress over and above the effect of prehurricane psychological functioning and disaster exposure. Gains in resources showed no effect. Implications for practice and policy were discussed.
Consensus QSAR Related to Global or MOA Models: Application to Acute Toxicity for Fish
Authors: Lozano Sylvain , Halm‐Lemeille Marie‐Pierre , Lepailleur Alban , Rault Sylvain , Bureau Ronan 
Published: November 2010

AbstractUnder REACH legislation, alternative methods (in silico or in vitro) like QSAR (Quantitative Structure‐Activity Relationships) models are expected to play a significant role. QSARs are based on the assumption that substances with similar chemical structures may have the same biological activities. However, identification of chemical classes could be problematic because chemicals often exhibit different chemical moieties, thereby confounding efforts to achieve a meaningful classification. This publication is focus on the notion of global model with the integration of a recent genetic algorithm for the generation of QSAR models. Starting from three datasets (EPAFHM, ECBHPV, AQUIRE), prediction of acute toxicity for fish (Pimephales promelas) with a global consensus model was carried out leading to very interesting statistics. The integration of the notion of Mode of Action was the second point of this study. A Bayesian classification associated to the genetic algorithm for consensus models was created leading to a good estimation of toxicity associated to derivatives with nonspecific MOA.
9‐oxo‐10(E),12(E)‐octadecadienoic acid derived from tomato is a potent PPAR α agonist to decrease triglyceride accumulation in mouse primary hepatocytes
Authors: Kim Young‐Il , Hirai Shizuka , Takahashi Haruya , Goto Tsuyoshi , Ohyane Chie , Tsugane Taneaki , Konishi Chiaki , Fujii Takashi , Inai Shuji , Iijima Yoko , Aoki Koh , Shibata Daisuke , Takahashi Nobuyuki , Kawada Teruo 
Published: November 2010

AbstractScope: Tomato is one of the most common crops worldwide and contains many beneficial compounds that improve abnormalities of lipid metabolism. However, the molecular mechanism underlying the effect of tomato on lipid metabolism is unclear. It has been commonly accepted that peroxisome proliferator‐activated receptor α (PPARα) is one of the most important targets for ameliorating abnormalities of lipid metabolism. Therefore, we focused on the activation of PPARα and attempted to detect active compounds activating PPARα in tomato.Methods and results: To identify such active compounds, we screened fractions of tomato extracts using PPARα luciferase reporter assay. One fraction, rechromatographed‐fraction eluted in 57 min (RF57), significantly increased PPARα reporter activity, in which a single compound is detected by LC/MS analysis. On the basis of LC/MS and NMR analyses, we determined the chemical structure of the active compound in RF57 as 9‐oxo‐10(E),12(E)‐octadecadienoic acid (9‐oxo‐ODA). The RF57 fraction significantly increased the mRNA expression levels of PPARα target genes involved in fatty acid oxidation and O2 consumption in mouse primary hepatocytes. Furthermore, RF57 inhibited cellular triglyceride accumulation in the hepatocytes.Conclusion: These findings suggest that tomatoes containing 9‐oxo‐ODA that acts on PPARα are valuable for ameliorating abnormalities of lipid metabolism.