bluuebunny/crossref_metadata_2025_split huggingface dataset:
Activity of deacetylase inhibitor panobinostat (LBH589) in cutaneous T‐cell lymphoma models: Defining molecular mechanisms of resistance
Authors: Shao Wenlin , Growney Joseph D. , Feng Yun , O'Connor Gregory , Pu Minying , Zhu Wenjing , Yao Yung‐Mae , Kwon Paul , Fawell Stephen , Atadja Peter 
Published: February 2010

AbstractPanobinostat (LBH589) is a highly potent deacetylase inhibitor that has demonstrated clinical efficacy in patients with advanced cutaneous T‐cell lymphoma (CTCL). To gain a better understanding of the compound activity in this tumor type, we investigated the cellular and molecular effects of panobinostat using both in vitro and in vivo models of CTCL. All 4 tested CTCL cell lines exhibited very high sensitivity to panobinostat‐induced growth inhibition. However, only 2 of 4 lines exhibited significant response to the cytotoxic activity of panobinostat. In a CTCL xenograft mouse tumor model, panobinostat treatment resulted in complete tumor regression. The difference in cell sensitivity to panobinostat‐induced death enabled us to further investigate potential mechanisms responsible for tumor sensitivity or resistance. In CTCL cell lines that were insensitive to panobinostat‐induced apoptosis, constitutively activated NF‐κB and high levels of Bcl‐2 were observed. Inhibition of Bcl‐2 sensitized cells to the cytotoxic activity of panobinostat. Conversely, knockdown of Bax diminished the CTCL cell sensitivity. Interestingly, panobinostat could induce cytotoxicity in vorinostat‐resistant CTCL cells by downregulating phosphorylated STAT3 and STAT5 proteins. These studies suggest distinct mechanisms responsible for resistance to different deacetylase inhibitors. We show that the intrinsic apoptotic signaling plays an essential role in mediating panobinostat anticancer activity. Moreover, cancer cell sensitivity to panobinostat treatment may be further improved by combination with inhibition of anti‐apoptotic factors. These data provide preclinical support that panobinostat, as a single agent or in combination with other anticancer agents, is a promising therapy for CTCL.
Kindlin‐2 is expressed in malignant mesothelioma and is required for tumor cell adhesion and migration
Authors: An Zhengwen , Dobra Katalin , Lock John G. , Strömblad Staffan , Hjerpe Anders , Zhang Hongquan 
Published: February 2010

AbstractKindlin‐2 is a novel integrin‐interacting focal adhesion protein that belongs to the Kindlin family. Focal adhesion proteins control cytoskeleton dynamics and promote cancer cell growth, survival, migration and metastasis. Little is known, however, about expression of Kindlin‐2 in association with human cancer. We now reveal high Kindlin‐2 expression in malignant mesothelioma (MM) cell lines using an affinity‐purified anti‐Kindlin‐2 antibody. Furthermore, we show by immunohistochemistry that Kindlin‐2 is highly expressed in 92 of 102 (90%) MMs with epitheliod; sarcomatoid, biphasic and poorly differentiated morphologies. In addition, Kindlin‐2 expression correlates to cell proliferation, suggesting a role for Kindlin‐2 in tumor growth. We also detect increased expression of Kindlin‐2 at the invasion front of tumors concurrent with increased expression of integrin‐linked kinase, a Kindlin‐binding protein. Besides the high expression of Kindlin‐2 in pleural MMs, pleural metastases of lung adenocarcinoma also express large amounts of Kindlin‐2, but not Kindlin‐1. Notably, in vitro, when endogenous Kindlin‐2 was knocked down with RNAi in MM cells, this impaired cell spreading, adhesion and migration. Overall, our study suggests that heightened expression of Kindlin‐2 might contribute to tumor progression in MM.
In vitro evaluation of samarium (III) oxide as a bone substituting material
Authors: Herath H. M. T. U. , Di Silvio L. , Evans J. R. G. 
Published: February 2010

AbstractThe biocompatibility of natural samarium (III) oxide, which has previously been used for treatment in bone‐related diseases was determined as a first step in its evaluation as a bone implant material. Assessment for 28 days using osteoblast‐like cells revealed no indications of cytotoxicity. The cells adhered and proliferated on the surface. Furthermore, the differentiation and mineralization were observed, indicating a normal biological response of the cells on the samarium (III) oxide surface. The in vitro, short term biocompatibility assessment of this oxide has indicated its biosafety with no damaging toxic effects on the cells and biofunctionality; with an appropriate cell response for a bone‐contacting material. Hence, samarium (III) oxide deserves recognition in the field of biomaterials for its excellent in vitro performance and demonstrates that the class of potential bioceramics may be larger than previously thought. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Electrochemical behavior of cobalt–chromium alloys in a simulated physiological solution
Authors: Pound Bruce G. 
Published: February 2010

AbstractSeveral cobalt–chromium alloys such as MP35N are used for biomedical implants. The electrochemical behavior of these alloys in the passive range differs from that of other biomedical alloys. In particular, their cyclic potentiodynamic polarization curves exhibit an increase in current at a potential of about 0.4 V (SCE). This study examined the electrochemical behavior of MP35N in phosphate‐buffered saline (PBS). Tests were performed on mechanically polished MP35N rod and electropolished MP35N wire using cyclic potentiodynamic polarization, potentiostatic polarization, and electrochemical impedance spectroscopy. In both cases, the surface oxide was shown to undergo two distinct changes as the potential is increased. The first change appears to be associated with solid‐state oxidation reactions involving the conversion of Cr(III) to Cr(VI) and of Co(II) to Co(III) at similar potentials. The second change involves transpassive dissolution that results in the release of chromate and nickel ions. At potentials below where these reactions occur, the impedance spectra showed near‐capacitive behavior, and the data could be fitted by a parallel resistance‐capacitance (as a constant phase element) circuit associated with the passive oxide film. The thickness of the oxide was determined from the capacitance and found to be consistent with surface analytical results reported in the literature. Resistivities obtained from resistance values indicated changes in the film composition as the potential was increased, particularly after the onset of the solid‐state reactions. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res 2010
Biomechanical implant fixation of CoCrMo coating inferior to titanium coating in a canine implant model
Authors: Jakobsen Stig S. , Baas Jorgen , Jakobsen Thomas , Soballe Kjeld 
Published: February 2010

AbstractThe use of CoCrMo in orthopedic surgery is not new, and CoCrMo (cobalt–chromium–molybdenum) is well tolerated. Nevertheless, the alloy is still considered less biocompatible than titanium. We therefore wanted to explore the biocompatibility of CoCrMo by investigating the biomechanical implant fixation and implant osseointegration of CoCrMo (ASTM F‐75) porous bead‐coated and titanium (ASTM F‐136) porous bead‐coated implants. In 10 dogs, the two implant types were inserted into the proximal part of the humerus. Implant sites were overdrilled, leaving an empty 0.75‐mm gap between implant and surrounding bone. The implants were observed for 6 weeks and were evaluated by biomechanical push‐out test and histomorphometry. We found a statistically significant 40% decrease in the biomechanical fixation of CoCrMo porous bead‐coated implants compared with titanium porous bead‐coated implants. Implant osseointegration was comparable between the two implants; however, a slight decrease in bone volume density around CoCrMo implants was observed. Insertions of CoCrMo implants are associated with a disturbance of the delicate peri‐implant milieu. Even from implants not subjected to any mechanical forces, metal ions are liberated and result in intra‐ and extracellular accumulation in the immediate implant vicinity, presenting a likely explanation for our findings. A 40% reduction of initial implant fixation could prove to be serious because initial implant fixation is critical for long‐term performance. The choice between titanium alloy and CoCrMo should, however, ultimately be governed by a comprehensive review of all factors influencing clinical implant survival. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
A physiologically active polysaccharide hydrogel promotes wound healing
Authors: Luo Yi , Diao Huajia , Xia Suhua , Dong Lei , Chen Jiangning , Zhang Junfeng 
Published: February 2010

AbstractWhen the skin is injured, the subcutaneous tissues and organs are threatened by pathogens and excessive water loss. Wound dressings are, therefore, needed to protect the wound site from infection and improve the wound closure. Natural polysaccharides have been applied for various biomaterials including wound dressings, which show their advantages in biocompatibility, low toxicity, and pharmaceutical biomedical activity. In this study, a natural polysaccharide Bletilla striata polysaccharide (BSP) hydrogel is prepared by an oxidation and crosslinking methods. This BSP hydrogel represents preferable swelling ability and appropriate water vapor transmission rate. Using a full‐thickness trauma mouse model, the hydrogel is applied on the in vivo cutaneous wound healing. Compared with the control groups, the BSP hydrogel achieves the much better healing results. The quantification of the infiltrating inflammatory cells and the level of tumor necrosis factor alpha (TNF‐α) in the BSP group are attenuated, whereas the secretion of the epidermal growth factor (EGF) is highly elevated. On the 11th day after surgery, the wound area in the BSP hydrogel group is only 1/5–1/3 of those in the control groups. This new BSP hydrogel is proved to control the inflammatory responses and accelerate the wound closure and has potential application in wound healing. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Stability of poly(3,4‐ethylene dioxythiophene) materials intended for implants
Authors: Thaning Elin M. , Asplund Maria L. M. , Nyberg Tobias A. , Inganäs Olle W. , von Holst Hans 
Published: February 2010

AbstractThis study presents experiments designed to study the stability of the conducting polymer poly(3,4‐ethylene dioxythiophene) (PEDOT), under simulated physiological conditions using phosphate‐buffered saline (PBS) and hydrogen peroxide (H2O2) (0.01M) at 37°C over a 5‐ to 6‐week period. Voltage pulsing in PBS was used as an additional test environment. The influence of switching the counter ion used in electropolymerization from polystyrene sulphonate (PSS) to heparin was investigated. Absorbance spectroscopy and cyclic voltammetry were used to evaluate the material properties. Most of the samples in H2O2 lost both electroactivity and optical absorbance within the study period, but PEDOT:PSS was found slightly more stable than PEDOT:heparin. Polymers were relatively stable in PBS throughout the study period, with around 80% of electroactivity remaining after 5 weeks, disregarding delamination, which was a significant problem especially for polymer on indium tin oxide substrates. Voltage pulsing in PBS did not increase degradation. The counter ion influenced the time course of degradation in oxidizing agents. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2010
Color Doppler sonographic evaluation of flow volume of the internal carotid and vertebral arteries after carotid endarterectomy
Authors: Mitrasinovic Anka , Radak Sandra , Kolar Jovo , Aleksic Nikola , Otasevic Petar , Popovic Mirjana , Radak Djordje 
Published: February 2010

AbstractBackground.To measure by Doppler sonography the blood flow volume (BFV) of the ipsilateral and contralateral extracranial internal carotid arteries (ICAs) and both vertebral arteries (VAs) before and after a carotid endarterectomy (CEA) of the ICA. We correlated the result with the degree of stenosis of the ICA.Method.One hundred seven patients who had a CEA were divided into 2 groups. Group I consisted of subjects with stenosis of ipsilateral ICA of ≥70% to near occlusion and Group II included subjects with near occlusion. The Doppler sonographic examinations were performed 1 day before the CEA, 7 days after the CEA, and 1 month after the CEA. The peak systolic velocity, end‐diastolic velocity, time‐averaged maximum blood flow velocity, resistance index of the ipsilateral ICA, and the BFV of both ICAs and both VAs were calculated.Result.There was a significant increase in the peak systolic velocity, maximum blood flow velocity, and the BFV of the ipsilateral ICA after the CEA. The BFV of the contralateral ICA and both VAs were not significantly altered after the CEA in both groups.Conclusion.The main CEA hemodynamic effect was an increase in the BFV of the ipsilateral ICA regardless of the degree of stenosis. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound, 2010
Reliability study of the sonographic measurement of the acromiohumeral distance in symptomatic patients
Authors: Pijls B. G. , Kok F. P. , Penning L. I. F. , Guldemond N. A. , Arens H. J. 
Published: February 2010

AbstractBackground.The purpose of this study was to evaluate the inter‐ and intra‐observer reliability and accuracy of sonographic (US) acromiohumeral distance (AHD) measurement for both experienced and novice operators in US in patients suffering from subacromial impingement syndrome.Method.A total of 43 patients (50 shoulders) diagnosed with subacromial impingement syndrome were recruited from an orthopedic outpatient clinic. The US measurement of AHD was obtained consecutively in a neutral position and in a 60° abduction position. A total of 300 blinded measurements were taken.Result.In the neutral abduction group the intra‐ observer interclass correlation coefficient (ICC) was 0.94 for the experienced operator and 0.92 for the novice operator. The inter‐observer ICC was 0.70 and the accuracy was 1.1 mm. In the 60° abduction group, the intra‐observer ICC was 0.90 for the experienced operator and 0.87 for the novice operator. The inter‐observer ICC was 0.64 and the accuracy was 1.4 mm. All ICCs were significant at a level of p < 0.0001.Conclusion.The inaccuracy of the method was 1 mm regardless of the experience of the observer. US AHD measurement in patients with shoulder complaints is not as accurate as reported in healthy subjects. This may have important implications for the clinical use of this parameter. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound, 2010
Immature animals have higher cellular density in the healing anterior cruciate ligament than adolescent or adult animals
Authors: Mastrangelo Ashley N. , Haus Brian M. , Vavken Patrick , Palmer Matthew P. , Machan Jason T. , Murray Martha M. 
Published: February 2010

AbstractThere has been recent interest in the biologic stimulation of anterior cruciate ligament (ACL) healing. However, the effect of age on the ability of ligaments to heal has not yet been defined. In this study, we hypothesized that skeletal maturity would significantly affect the cellular and vascular repopulation rate of an ACL wound site. Skeletally Immature (open physes), Adolescent (closing physes), and Adult (closed physes) Yucatan minipigs underwent bilateral ACL transection and suture repair using a collagen‐platelet composite. The response to repair was evaluated histologically at 1, 2, and 4 weeks. All three groups of animals had completely populated the ACL wound site with fibroblasts at 1 week. The Immature animals had a higher cellular density in the wound site than the Adult animals at weeks 2 and 4. Cells in the Immature ligament wounds were larger and more ovoid than in the Adult wounds. There were no significant differences in the vascular density in the wound site. Animal age had a significant effect on the density of cells populating the ACL wound site. Whether this observed cellular difference has an effect on the later biomechanical function of the repaired ACL requires further study. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1100–1106, 2010
Enhanced water absorption of wheat gluten by hydrothermal treatment followed by microbial transglutaminase reaction
Authors: Liu Song , Zhang Dongxu , Liu Long , Wang Miao , Du Guocheng , Chen Jian 
Published: February 2010

AbstractBACKGROUND: The water absorption of wheat gluten plays an important role in the weight, volume and form ratio of the breads. In this paper, hydrothermal treatment and microbial transglutaminase (MTGase) modification were combined to improve the water absorption ratio (WAR) of wheat gluten. To understand the increases in WAR, the changes in MTGase reaction after gluten hydrothermal treatment were also investigated.RESULTS: The sole hydrothermal treatment improved the WAR of gluten. The gluten treated at 100 °C for 30 min exhibited the highest WAR value (2.03 g g−1 gluten) while the WAR of the control without hydrothermal treatment was 1.5 g g−1 gluten. When gluten was exposed to 90 °C for 30 min followed by incubation with MTGase for 5 h, its WAR reached 2.48 g g−1 gluten. In contrast to control gluten, the surface hydrophobicity of the gluten preheated at 90 °C for 30 min increased and fluctuated in a different way during the following MTGase reaction. Meantime, the trend in the amount of soluble protein of preheated gluten was also changed in the progress of MTGase reaction.CONCLUSION: Hydrothermal treatment followed by MTGase reaction is an efficient approach to improve the WAR of wheat gluten. The analysis of catalytic process, including determination of ammonia, gluten surface hydrophobicity, soluble protein and SDS–PAGE, suggested that hydrothermal pretreatment accelerated the cross‐linking reaction and may alter the ratio of gluten deamidation catalysed by MTGase, which induced an increase in the WAR. Copyright © 2010 Society of Chemical Industry
Surgical resection for pulmonary metastasis from hepatocellular carcinoma: Analysis of prognosis in relation to primary control
Authors: Lee Chang Young , Bae Mi Kyung , Park In Kyu , Kim Dae Joon , Lee Jin Gu , Choi Jin Sub , Han Kwang‐Hyub , Chung Kyung Young 
Published: February 2010

AbstractBackgroundAdvances in local treatments for hepatocellular carcinoma (HCC) have seen better prognosis. There were few studies on surgery for pulmonary metastasis from HCC controlled by local treatment. This study was conducted to analyze prognostic factors after surgery for pulmonary metastasis from HCC.MethodsWe reviewed 32 patients who underwent surgery for pulmonary metastasis from HCC and analyzed prognostic factors.ResultsHCC was controlled by surgery in 16 patients and by local treatment in 16 patients at the time of pulmonary resection. The median survival time after pulmonary resection was significantly better for HCC controlled by surgery, disease‐free time ≥12 months, TMN stage I–II, and T factor 1–2 of primary HCC. The median survival time was better for lesion ≤2 and Child–Pugh score A, but they were not significant (P = 0.053, 0.069). In multivariate analysis, HCC controlled by surgical treatment (P = 0.008), and lesion ≤2 (P = 0.030) were independent prognostic factors.ConclusionOur Results indicated that surgical resection for pulmonary metastasis from HCC might be beneficial for patients whose HCC was controlled by surgery and the number of lesions was lower than 3. J. Surg. Oncol. 2010; 101:239–243. © 2010 Wiley‐Liss, Inc.
Clinical significance of the discrepancy between preoperative and postoperative diagnoses in gastric cancer patients
Authors: Shin Suk Hee , Bae Jae Moon , Jung Hun , Choi Min Gew , Lee Jun Haeng , Noh Jae Hyung , Sohn Tae Sung , Kim Sung 
Published: February 2010

AbstractBackgroundDiscrepancies between pre‐ and postoperative diagnoses can lead to dilemma for operative management adequacy.Patients and MethodsA total of 2,910 patients with gastric adenocarcinoma underwent curative surgery at the Samsung Medical Center between 2001 and 2003. Patients were divided into four groups: early gastric cancer (EGC)–EGC group that consisted of subjects who were diagnosed as having EGC pre‐ and postoperatively, advanced gastric cancer (AGC)–EGC group, EGC–AGC group, and AGC–AGC group. Clinicopathologic features and survival rates of groups were analyzed retrospectively.ResultsOf the 2,910 patients, 1,491 (51.2%) patients were included in the EGC–EGC group, 132 (4.5%) in the AGC–EGC group, 120 (4.1%) in the EGC–AGC group, and 1,167 (40.1%) in the AGC–AGC group. The EGC–AGC group showed higher proportions of the followings than the EGC–EGC group: upper‐third and middle‐third tumor localizations, a tumor size from 2 to 5 cm, undifferentiated adenocarcinoma, Lauren's diffuse type, endolymphatic invasion, vascular invasion, and perineural invasion. Five‐year survival rates were dependent on the final pathologic stages, not on the preoperative stages. Multivariate analysis revealed that age and American Joint Committee of Cancer stage were independent prognostic factors of patient survival.ConclusionA decision regarding minimally invasive treatment for EGC must be made having considered tumor location, size, and cellular differentiation, because of the possibility of an incorrect preoperative diagnosis. J. Surg. Oncol. 2010; 101:384–388. © 2010 Wiley‐Liss, Inc.
Electrogenerated ferrate(VI) for CE–chemiluminescence detection to determine benzenediol isomers
Authors: Li Fang , Hu Yonggang , Zhang Huijing , Zhang Jie 
Published: February 2010

AbstractBased on the quenched chemiluminescence intensity of the luminol reaction sensitized by ferrate(VI) in alkaline medium, a novel CE with on‐line inhibited method for the simultaneous analysis of benzenediol isomers was developed. The ferrate(VI) solution was freshly prepared by electrochemical technique before electrophoresis. The parameters influencing detection and separation were carefully investigated. Baseline separation of benzenediols including catechol, resorcinol, and hydroquinone was achieved in less than 8 min with 5.0 mmol/L sodium tetraborate and 2.0 mmol/L luminol at an applied voltage of 18 kV. The LODs (S/N=3) for catechol, resorcinol and hydroquinone were determined to be 8.5×10−9, 8.0×10−7 and 6.5×10−9 mol/L, respectively. Finally, the proposed method was applied for phenolic compounds in hair dye.
Determination of aflatoxins B1, B2, G1, G2 and ochratoxin A in animal feed by ultra high‐performance liquid chromatography–tandem mass spectrometry
Authors: López Grío Sergio José , Garrido Frenich Antonia , Martínez Vidal José Luis , Romero‐González Roberto 
Published: February 2010

AbstractA rapid and simple method was developed to determine aflatoxins B1, B2, G1, G2 and ochratoxin A in animal feed and pet foods by UHPLC–MS/MS. Because the complexity of the evaluated matrices, the proposed method is based on sonication extraction using an ACN/water mixture (80:20 v/v) followed by a clean‐up step utilising C18 as sorbent. Performance parameters of the method were evaluated, including linearity, trueness, precision and LOQ. Good linearity was found for all mycotoxins, with determination coefficients higher than 0.99 in the range considered, using matrix‐matched calibration for quantification purposes. Recoveries ranged from 84 to 113%, with RSD lower than 20%, whereas LOQs were 5 μg/kg for the assayed mycotoxins. Finally, the method was successfully applied to the analysis of 19 real samples, detecting aflatoxin G2 in two samples at 13 and 17 μg/kg respectively, whereas the other mycotoxins were detected at trace levels (<LOQ) in five samples.