bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hepatitis C virus and GBV‐C virus prevalence among patients with B‐cell lymphoma in different European regions: a case‐control study of the International Extranodal Lymphoma Study Group
Authors: Nicolosi Guidicelli Sabrina , Lopez‐Guillermo Armando , Falcone Umberto , Conconi Annarita , Christinat Alexandre , Rodriguez‐Abreu Delvys , Grisanti Salvatore , Lobetti‐Bodoni Chiara , Piffaretti Jean Claude , Johnson Peter W. , Mombelli Giorgio , Cerny Andreas , Montserrat Emili , Cavalli Franco , Zucca Emanuele 
Published: November 2011

AbstractHepatitis C virus (HCV) infection is associated with some B‐cell non‐Hodgkin lymphoma (B cell‐NHLs). Patients with HCV infection frequently show co‐infections with GB virus C (GBV‐C, formerly known as hepatitis G virus), and some studies have suggested a higher incidence of GBV‐C infection in patients with B cell‐NHLs. The aim of this study was to prospectively evaluate the association between HCV and/or GBV‐C infection and B cell‐NHLs in different geographic areas. One hundred thirty‐seven lymphoma cases and 125 non‐lymphoma matched controls were enrolled in an international case‐control study conducted in Switzerland (Bellinzona), Spain (Barcelona) and England (Southampton) on samples collected from 2001 to 2002. In Bellinzona (41 cases and 81 controls), the overall prevalence of HCV was 3.3% (4.9% in NHLs), and the overall prevalence of GBV‐C was 24% (22% in NHLs). In Barcelona (46 cases and 44 controls), the prevalence of HCV was 10% (8.7% in NHLs) and the prevalence of GBV‐C 20% (13% in NHLs). There was no statistically significant difference in the frequency of both infections between patients with NHL and controls. In Southampton, 50 NHL cases were analysed, none of them was found to be HCV‐positive; therefore, no control group was analysed and GBV‐C analysis was not performed, too. Both in Bellinzona and in Barcelona, the seropositivity rate was significantly lower for HCV than for GBV‐C, suggesting that their transmission can be independent. The incidence of HCV was significantly higher in Barcelona than that in Bellinzona. This study confirmed the existence of marked geographic differences in the prevalence of HCV in NHL but cannot provide any significant evidence for an association between HCV and/or GBV‐C and B‐cell NHLs. Copyright © 2011 John Wiley & Sons, Ltd.
The effects of agomelatine on sexual function in depressed patients and healthy volunteers
Authors: Montejo Angel , Majadas Susana , Rizvi Sakina J. , Kennedy Sidney H. 
Published: November 2011

BackgroundSelective serotonin reuptake inhibitor (SSRI) and serotonin and norepinephrine reuptake inhibitor antidepressants are associated with high rates of treatment‐emergent sexual dysfunction (TESD) due to stimulation of serotonin receptors.ObjectiveThe objective is to evaluate the effect of agomelatine on sexual function in depressed patients.MethodsThis paper reviews published and unpublished data on sexual function with agomelatine in depressed patients and healthy volunteers.ResultsAgomelatine, an agonist of melatonergic MT1 and MT2 receptors and antagonist of 5‐HT2 receptors, is associated with similar rates of sexual dysfunction compared with placebo and lower rates compared with other antidepressants. Twice as many sexually active depressed patients (n = 193) reported a deterioration of sexual function during 12 weeks of treatment with venlafaxine compared with agomelatine (15.2% vs. 8.2%, p < 0.0001); however, no differences were found with respect to arousal. Using the Arizona Sexual Experience Scale in depressed patients (n = 399), the incidence of treatment‐emergent sexual dysfunction (TESD) with agomelatine (3%) was significantly lower than placebo (8.6%) and selective serotonin reuptake inhibitors (10.1%). Among healthy male volunteers (n = 92), TESD was not increased compared with placebo in either agomelatine (25 and 50 mg/day) group over 8 weeks, and both were significantly lower than TESD with paroxetine (p < 0.0001). Moderate or severe TESD occurred in less than 5% of subjects receiving agomelatine versus 62% who received paroxetine (p < 0.001).ConclusionAgomelatine demonstrates favorable sexual acceptability. Copyright © 2011 John Wiley & Sons, Ltd.
GMM with many weak moment conditions: Replication and application of Newey and Windmeijer (2009)
Authors: Farbmacher Helmut 
Published: November 2011

SUMMARYIn a recent article Newey and Windmeijer (Generalized method of moments with many weak moment conditions. Econometrica 2009; 77(3): 687–719) propose a new variance estimator for generalized empirical likelihood. In Monte Carlo examples they show that t‐statistics based on the new variance estimator have nearly correct size. I have replicated their Monte Carlo simulations and in addition used the new variance estimator to re‐estimate Angrist and Krueger's (Does compulsory school attendance affect schooling and earnings? Quarterly Journal of Economics 1991; 106(4): 979–1014) returns to education. Copyright © 2011 John Wiley & Sons, Ltd.
Alignment and composition of laminin–polycaprolactone nanofiber blends enhance peripheral nerve regeneration
Authors: Neal Rebekah A. , Tholpady Sunil S. , Foley Patricia L. , Swami Nathan , Ogle Roy C. , Botchwey Edward A. 
Published: November 2011

AbstractPeripheral nerve transection occurs commonly in traumatic injury, causing deficits distal to the injury site. Conduits for repair currently on the market are hollow tubes; however, they often fail due to slow regeneration over long gaps. To facilitate increased regeneration speed and functional recovery, the ideal conduit should provide biochemically relevant signals and physical guidance cues, thus playing an active role in regeneration. To that end, laminin and laminin–polycaprolactone (PCL) blend nanofibers were fabricated to mimic peripheral nerve basement membrane. In vitro assays established 10% (wt) laminin content is sufficient to retain neurite‐promoting effects of laminin. In addition, modified collector plate design to introduce an insulating gap enabled the fabrication of aligned nanofibers. The effects of laminin content and fiber orientation were evaluated in rat tibial nerve defect model. The lumens of conduits were filled with nanofiber meshes of varying laminin content and alignment to assess changes in motor and sensory recovery. Retrograde nerve conduction speed at 6 weeks was significantly faster in animals receiving aligned nanofiber conduits than in those receiving random nanofiber conduits. Animals receiving nanofiber‐filled conduits showed some conduction in both anterograde and retrograde directions, whereas in animals receiving hollow conduits, no impulse conduction was detected. Aligned PCL nanofibers significantly improved motor function; aligned laminin blend nanofibers yielded the best sensory function recovery. In both cases, nanofiber‐filled conduits resulted in better functional recovery than hollow conduits. These studies provide a firm foundation for the use of natural–synthetic blend electrospun nanofibers to enhance existing hollow nerve guidance conduits. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
Design and assessment of a wrapped cylindrical Ca‐P AZ31 Mg alloy for critical‐size ulna defect repair
Authors: Smith Montserrat Rabago , Atkinson Patrick , White Désirée , Piersma Tyler , Gutierrez Gloria , Rossini Gianny , Desai Sapna , Wellinghoff Stephen , Yu Hui , Cheng Xingguo 
Published: November 2011

AbstractRecently, magnesium has been investigated as a promising bioresorbable orthopedic biomaterial. Its mechanical properties are very similar to natural bone, making it appropriate for load‐bearing orthopedic fracture repair applications. However, significant hurdles remain regarding the design of practical implants and methods to control degradation and enhance biocompatibility. Although attempts have been made to hinder magnesium's rapid corrosion via alloying and coating, these studies have used solid monoliths. In an effort to reduce the amount of alloy used for implantation in a shape that mimics cortical bone shape, this study used a thin sheet of Mg AZ31 which was rolled into hollow cylindrical scaffolds. The scaffold was coated with different amounts of Ca‐P; this implant demonstrated slowed corrosion in simulated body fluid (SBF) as well as enhanced biocompatibility for mesenchymal stem cells (MSC). In vivo implantation of magnesium alloy scaffold adjacent to the rat femur showed significant biointegration with further deposition of complex Mg‐Ca phosphates/carbonates typical of natural bone. Finally, the implant was placed in a critical‐size ulna defect in live rabbits, which lead to radiographic union and partial restoration of biomechanical strength in the defect. This study demonstrated that a thin sheet of coated Mg alloy that was spirally wrapped wound be a promising orthopedic biomaterial for bone repair. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
Structural and in vitro adhesion analysis of a novel covalently coupled bioactive composite
Authors: Khan Abdul S. , Hassan Khawaja R. , Bukhari Syeda F. , Wong Ferranti S. L. , Rehman Ihtesham U. 
Published: November 2011

AbstractThe interfacial adhesion between a restorative composite and tooth is one of the major factors that determine the ultimate performance of composite restoration. A novel polyurethane (PU) composite material was prepared by chemically binding the nano‐hydroxyapatite (nHA) to the diisocyanate component in the PU backbone by utilizing solvent polymerization. The procedure involved stepwise addition of monomeric units of the PU and optimizing the reagent concentrations. The resultant materials were characterized structurally (Raman Spectroscopy) and in vitro bioactive analysis was conducted in modified‐simulated body fluid for periodical time intervals. The in vitro study evaluated the push‐out bond strength of existing obturating material and novel covalently linked PU/nHA composites to dentin after long‐term storage in deionized water and artificial saliva. Human extracted molar roots were filled with experimental samples and analyzed at predetermined time intervals. The shear bond strength of samples was measured and surface morphologies were evaluated. Covalent bond formation was achieved between PU and nHA without intermediate coupling agent. With the increase in concentration of nHA, the composite showed more bioactivity and adhesion toward tooth structure. Bond strength of this new composite were in accordance with obutrating material, therefore, the material can be used as an obturating material because of its direct adhesion with tooth structure. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
Synthesis and characterization of silver/silicon‐cosubstituted nanohydroxyapatite
Authors: Lim Poon Nian , Tay Bee Yen , Chan Cynthia Mingli , Thian Eng San 
Published: November 2011

AbstractFavorable cell‐material interaction and the absence of undesirable reaction from the host body defence system play a critical role in determining the success and long‐term survival of the implants. Substitution of various elements into hydroxyapatite (HA) has been done to alter its chemical composition, thereby mimicking that of the bone mineral. In this study, a cosubstituted nanosized apatite (Ag/Si‐HA) containing Ag (0.3 wt %) and Si (0.8 wt %) was synthesized by an aqueous precipitation technique. The synthesized Ag/Si‐HA displayed a rod‐like morphology of dimensions ∼50 nm in length and ∼15 nm in width, as observed from the transmission electron microscope image. With an increase in temperature, the aspect ratio of nanosized Ag/Si‐HA decreased, whilst the size increased. Autoclaving was used to achieve sufficient crystallinity while maintaining the rod‐like morphology and size that were comparable to that of the bone apatite. A pure Ag/Si‐HA was produced without any undesirable secondary phases, as evidenced from the X‐ray diffraction and thermal gravimetric results. The Ag/Si cosubstitution affected the lattice cell parameters, in particularly the a‐ and c‐ axes which further led to an expansion of the unit cell volume. In addition, the relative intensity of the hydroxyl vibrational bands was reduced. These results demonstrated that a stable phase‐pure Ag/Si‐HA was produced using an aqueous precipitation reaction. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
Fibrous membrane of nano‐hybrid poly‐L‐lactic acid/silica xerogel for guided bone regeneration
Authors: Jang Tae‐Sik , Lee Eun‐Jung , Jo Ji‐Hoon , Jeon Jong‐Myeong , Kim Mi‐Young , Kim Hyoun‐Ee , Koh Young‐Hag 
Published: November 2011

AbstractNanofibrous membranes, consisting of a poly(L‐lactic acid) (PLLA)‐silica xerogel hybrid material, were successfully fabricated from a hybrid sol using the electrospinning technique for guided bone regeneration (GBR) application. These hybrid nanofibers exhibited a homogeneous and continuous morphology, with a nano‐sized dispersed silica xerogel phase in the PLLA fiber matrix. The mechanical properties, such as the tensile strength and the elastic modulus, were improved as the silica xerogel content increased up to 40%. All of the hybrid membranes exhibited highly hydrophilic surfaces and good proliferation levels. After culturing for 13 days, the cells that were cultured on the hybrid membranes exhibited a significantly higher ALP activity compared to the pure PLLA membrane. Moreover, the in vivo animal experiments that used the rat calvarial defect model revealed a remarkably improved bone regeneration ability for the hybrid membrane compared to pure PLLA. These results demonstrated the feasibility of these hybrid membranes for efficient GBR. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 100B: 321–330, 2012.
Biological functionalization of dental implants with collagen and glycosaminoglycans—A comparative study
Authors: Stadlinger Bernd , Hintze Vera , Bierbaum Susanne , Möller Stephanie , Schulz Matthias C. , Mai Ronald , Kuhlisch Eberhard , Heinemann Sascha , Scharnweber Dieter , Schnabelrauch Matthias , Eckelt Uwe 
Published: November 2011

AbstractBiological implant surface coatings are an emerging technology to increase bone formation. Such an approach is of special interest in anatomical regions like the maxilla. In the present study, we hypothesized that the coating of titanium implants with components of the organic extracellular matrix increases bone formation and implant stability compared to an uncoated reference. The implants were coated using collagen‐I with either two different concentrations of chondroitin sulfate (CS) or two differentially sulfated hyaluronans. Implant coatings were characterized biochemically and with atomic force microscopy. Histomorphometry was used to assess bone‐implant contact (BIC) and bone‐volume density (BVD) after 4 and 8 weeks of submerged healing in the maxilla of 20 minipigs. Further, implant stability was measured by resonance frequency analysis (RFA). Implants containing the lower CS concentration had significantly more BIC, compared to the uncoated reference at both times of interest. No significant increase was measured from week 4 to 8. Differences in BVD and RFA were statistically not significant. A higher concentration of CS and the application of sulfated hyaluronans showed no comparable increase in BIC. This study demonstrates a positive effect of a specific collagen–glycosaminoglycan combination on early bone formation in vivo. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 100B: 331–341, 2012.
Long‐term outcomes of a chitosan hemostatic dressing in laparoscopic partial nephrectomy
Authors: Xie Hua , Lucchesi Lisa , Teach Jeffrey S. , Virmani Renu 
Published: November 2011

AbstractThis study examined the long‐term safety and effectiveness of a chitosan hemostatic dressing (CHD) in a porcine laparoscopic partial nephrectomy (LPN) model. Eighteen miniswine underwent treatment of using CHD or Surgicel® and Tisseel® (S/T) for renal parenchymal hemostasis after LPN. The animals were followed up for 6 and 12 months. Surgical procedure related complications, hematological and blood chemical changes were monitored. Histopathological examination was performed on the treated and untreated tissue and organs. All animals had initial hemostasis and survived without any immediate or delayed complications in both CHD and S/T groups. The animals with CHD treatment left large amounts of chitosan residuals on the resected kidney that associated with greater inflammatory scores when compared to the S/T treated animals. This long‐term study showed that CHD residuals remained for 12 months and resulted in local inflammation in the LPN model. Despite lack of signs and symptoms of clinical significance in the animals, a further investigation should be conducted to understand the risk of slow degradation of CHD. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 100B: 432–436, 2012.
Analysis of lipid surface area in protein–membrane systems combining voronoi tessellation and monte carlo integration methods
Authors: Mori Takaharu , Ogushi Fumiko , Sugita Yuji 
Published: November 2011

AbstractAll‐atom molecular dynamics (MD) simulation has become a powerful research tool to investigate structural and dynamical properties of biological membranes and membrane proteins. The lipid structures of simple membrane systems in recent MD simulations are in good agreement with those obtained by experiments. However, for protein–membrane systems, the complexity of protein–lipid interactions makes investigation of lipid structure difficult. Although the area per lipid is one of the essential structural properties in membrane systems, the area in protein–membrane systems cannot be computed easily by conventional approaches like the Voronoi tessellation method. To overcome this limitation, we propose a new method combining the two‐dimensional Voronoi tessellation and Monte Carlo integration methods. This approach computes individual surface areas of lipid molecules not only in bulk lipids but also in proximity to membrane proteins. We apply the method to all‐atom MD trajectories of the sarcoplasmic reticulum Ca2+‐pump and the SecY protein‐conducting channel. The calculated lipid surface area is in agreement with experimental values and consistent with other structural parameters of lipid bilayers. We also observe changes in the average area per lipid induced by the conformational transition of the SecY channel. Our method is particularly useful for examining equilibration of lipids around membrane proteins and for analyzing the time course of protein–lipid interactions. © 2011 Wiley Periodicals, Inc. J Comput Chem, 2012
Compromised decision making and the effects of manipulating physical states on human judgments
Authors: De Los Reyes Andres , Aldao Amelia , Kundey Shannon M.A. , Lee Bern G. , Molina Sabrina 
Published: November 2011

AbstractObjectives: Nonmorally based decision making between two equitable objects often involves degrading the unchosen object and promoting the chosen object (“postdecisional dissonance”). One can extinguish these thought processes with the physical act of hand‐washing (“clean slate” effects; [Lee & Schwarz (2010). Washing away postdecisional dissonance. Science, 328, 709.]). However, clean slate effects might not characterize all nonmorally based decision making, particularly for people who mentally “get stuck” making decisions (i.e., compromised decision making). Design: We administered a clean slate task to 48 undergraduates (64.6% females; mean = 21.34 years, standard deviation = 4.06 years; 75% Caucasian), and identified individuals reporting relatively high‐compromised versus low‐compromised decision making (e.g., self‐reported repetitive thought processes and generalized anxiety symptoms). Results: Only individuals reporting relatively high‐compromised decision making continued to express postdecisional dissonance even after hand‐washing. Conclusions: Behavioral markers of clean slate effects might result in identifying phenotypes associated with psychological concerns typified by compromised decision making. © 2011 Wiley Periodicals, Inc. J Clin Psychol 68:1–7, 2012.
Pharmaco‐metabolomics: An emerging “omics” tool for the personalization of anticancer treatments and identification of new valuable therapeutic targets
Authors: Corona Giuseppe , Rizzolio Flavio , Giordano Antonio , Toffoli Giuseppe 
Published: November 2011

AbstractIn the post‐genomics era, metabolomics represents a new “omics” approach that in the last decade has received increased attention in the field of oncology. Metabolomics is based on the holistic study of the metabolic profile that characterizes a specific phenotype in a biological system. The metabolic profile provides a readout of the metabolic state of an individual that cannot be obtained directly from DNA genotyping, gene expression, or proteomic profiling analyses. The translational value of metabonomics in the oncology field has been demonstrated by the identification of diagnostic and prognostic biomarkers. The so‐called pharmaco‐metabolomic approach that is currently emerging aims to identify the individual metabolomic characteristics able to predict drug effectiveness and/or toxicity. This review presents the potential role of pharmaco‐metabolomics in the future of anticancer pharmacology to achieve customized anticancer treatments and new, targeted therapeutic approaches. J. Cell. Physiol. 227: 2827–2831, 2012. © 2011 Wiley Periodicals, Inc.
Unsaturated fatty acids induce mesenchymal stem cells to increase secretion of angiogenic mediators
Authors: Smith Andria N. , Muffley Lara A. , Bell Austin N. , Numhom Surawej , Hocking Anne M. 
Published: November 2011

AbstractMesenchymal stem cells (MSC) represent emerging cell‐based therapies for diabetes and associated complications. Ongoing clinical trials are using exogenous MSC to treat type 1 and 2 diabetes, cardiovascular disease and non‐healing wounds due to diabetes. The majority of these trials are aimed at exploiting the ability of these multipotent mesenchymal stromal cells to release soluble mediators that reduce inflammation and promote both angiogenesis and cell survival at sites of tissue damage. Growing evidence suggests that MSC secretion of soluble factors is dependent on tissue microenvironment. Despite the contribution of fatty acids to the metabolic environment of type 2 diabetes, almost nothing is known about their effects on MSC secretion of growth factors and cytokines. In this study, human bone marrow‐derived MSC were exposed to linoleic acid, an omega‐6 polyunsaturated fatty acid, or oleic acid, a monounsaturated fatty acid, for seven days in the presence of 5.38 mM glucose. Outcomes measured included MSC proliferation, gene expression, protein secretion and chemotaxis. Linoleic and oleic acids inhibited MSC proliferation and altered MSC expression and secretion of known mediators of angiogenesis. Both unsaturated fatty acids induced MSC to increase secretion of interleukin‐6, VEGF and nitric oxide. In addition, linoleic acid but not oleic acid induced MSC to increase production of interleukin‐8. Collectively these data suggest that exposure to fatty acids may have functional consequences for MSC therapy. Fatty acids may affect MSC engraftment to injured tissue and MSC secretion of cytokines and growth factors that regulate local cellular responses to injury. J. Cell. Physiol. 227: 3225–3233, 2012. © 2011 Wiley Periodicals, Inc.