bluuebunny/crossref_metadata_2025_split huggingface dataset:
ArcKR expression modifies synaptic plasticity following epileptic activity: Differential effects with in vitro and in vivo seizure‐induction protocols
Authors: Bhandare Amol , Haley Maisy , Torrico Anderson Vanessa , Domingos Luana B. , Lopes Marcia , Corrêa Sonia A. L. , Wall Mark J. 
Published: May 2024

AbstractObjectivesPathological forms of neural activity, such as epileptic seizures, modify the expression pattern of multiple proteins, leading to persistent changes in brain function. One such protein is activity‐regulated cytoskeleton‐associated protein (Arc), which is critically involved in protein‐synthesis–dependent synaptic plasticity underlying learning and memory. In the present study, we have investigated how the expression of ArcKR, a form of Arc in which the ubiquitination sites have been mutated, resulting in slowed Arc degradation, modifies group I metabotropic glutamate receptor–mediated long‐term depression (G1‐mGluR‐LTD) following seizures.MethodsWe used a knock‐in mice line that express ArcKR and two hyperexcitation models: an in vitro model, where hippocampal slices were exposed to zero Mg2+, 6 mM K+; and an in vivo model, where kainic acid was injected unilaterally into the hippocampus. In both models, field excitatory postsynaptic potentials (fEPSPs) were recorded from the CA1 region of hippocampal slices in response to Schaffer collateral stimulation and G1‐mGluR‐LTD was induced chemically with the group 1 mGluR agonist DHPG.ResultsIn the in vitro model, ArcKR expression enhanced the effects of seizure activity and increased the magnitude of G1‐mGluR LTD, an effect that could be blocked with the mGluR5 antagonist MTEP. In the in vivo model, fEPSPs were significantly smaller in slices from ArcKR mice and were less contaminated by population spikes. In this model, the amount of G1‐mGluR‐LTD was significantly less in epileptic slices from ArcKR mice as compared to wildtype (WT) mice.SignificanceWe have shown that expression of ArcKR, a form of Arc in which degradation is reduced, significantly modulates the magnitude of G1‐mGluR‐LTD following epileptic seizures. However, the effect of ArcKR on LTD depends on the epileptic model used, with enhancement of LTD in an in vitro model and a reduction in the kainate mouse model.
Unearthing the soil‐borne microbiome of land plants
Authors: Ochoa‐Hueso Raúl , Eldridge David J. , Berdugo Miguel , Trivedi Pankaj , Sokoya Blessing , Cano‐Díaz Concha , Abades Sebastian , Alfaro Fernando , Bamigboye Adebola R. , Bastida Felipe , Blanco‐Pastor José L. , de los Rios Asunción , Durán Jorge , Geisen Stefan , Grebenc Tine , Illán Javier G. , Liu Yu‐Rong , Makhalanyane Thulani P. , Mamet Steven , Molina‐Montenegro Marco A. , Moreno José L. , Nahberger Tina Unuk , Peñaloza‐Bojacá Gabriel F. , Plaza César , Rey Ana , Rodríguez Alexandra , Siebe Christina , Singh Brajesh K. , Teixido Alberto L. , Torres‐Díaz Cristian , Wang Ling , Wang Jianyong , Wang Juntao , Zaady Eli , Zhou Xiaobing , Zhou Xin‐Quan , Tedersoo Leho , Delgado‐Baquerizo Manuel 
Published: May 2024

AbstractPlant–soil biodiversity interactions are fundamental for the functioning of terrestrial ecosystems. Yet, the existence of a set of globally distributed topsoil microbial and small invertebrate organisms consistently associated with land plants (i.e., their consistent soil‐borne microbiome), together with the environmental preferences and functional capabilities of these organisms, remains unknown. We conducted a standardized field survey under 150 species of land plants, including 58 species of bryophytes and 92 of vascular plants, across 124 locations from all continents. We found that, despite the immense biodiversity of soil organisms, the land plants evaluated only shared a small fraction (less than 1%) of all microbial and invertebrate taxa that were present across contrasting climatic and soil conditions and vegetation types. These consistent taxa were dominated by generalist decomposers and phagotrophs and their presence was positively correlated with the abundance of functional genes linked to mineralization. Finally, we showed that crossing environmental thresholds in aridity (aridity index of 0.65, i.e., the transition from mesic to dry ecosystems), soil pH (5.5; i.e., the transition from acidic to strongly acidic soils), and carbon (less than 2%, the lower limit of fertile soils) can result in drastic disruptions in the associations between land plants and soil organisms, with potential implications for the delivery of soil ecosystem processes under ongoing global environmental change.
Shifting the paradigm: The role of introduced plants in the resiliency of terrestrial ecosystems to climate change
Authors: Kharouba Heather M. 
Published: May 2024

AbstractCurrent ecological communities are in a constant state of flux from climate change and from species introductions. Recent discussion has focused on the positive roles introduced species can play in ecological communities and on the importance of conserving resilient ecosystems, but not how these two ideas intersect. There has been insufficient work to define the attributes needed to support ecosystem resilience to climate change in modern communities. Here, I argue that non‐invasive, introduced plant species could play an important role in supporting the resilience of terrestrial ecosystems to climate change. Using examples from multiple taxonomic groups and ecosystems, I discuss how introduced plants can contribute to ecosystem resilience via their roles in plant and insect communities, as well as their associated ecosystem functions. I highlight the current and potential contributions of introduced plants and where there are critical knowledge gaps. Determining when and how introduced plants are contributing to the resilience of ecosystems to climate change will contribute to effective conservation strategies.
Rising plant demand strengthens nitrogen limitation in tidal marsh
Authors: Langley J. A. , Wang L. , Yedman B. , Megonigal J. P. 
Published: May 2024

AbstractNitrogen (N) is a limiting nutrient for primary productivity in most terrestrial ecosystems, but whether N limitation is strengthening or weakening remains controversial because both N sources and sinks are increasing in magnitude globally. Temperate marshes are exposed to greater amounts of external N inputs than most terrestrial ecosystems and more than in preindustrial times owing to their position downstream of major sources of human‐derived N runoff along river mouths and estuaries. Simultaneously, ecosystem N demand may also be increasing owing to other global changes such as rising atmospheric [CO2]. Here, we used interannual variability in external drivers and variables related to exogenous supply of N, along with detailed assessments of plant growth and porewater biogeochemistry, to assess the severity of N‐limitation, and to determine its causes, in a 14‐year N‐addition × elevated CO2 experiment. We found substantial interannual variability in porewater [N], plant growth, and experimental N effects on plant growth, but the magnitude of N pools through time varied independently of the strength of N limitation. Sea level, and secondarily salinity, related closely to interannual variability in growth of the dominant plant functional groups which drove patterns in N limitation and in porewater [N]. Experimental exposure of plants to elevated CO2 and years with high flooding strengthened N limitation for the sedge. Abiotic variables controlled plant growth, which determined the strength of N limitation for each plant species and for ecosystem productivity as a whole. We conclude that in this ecosystem, which has an open N cycle and where N inputs are likely greater than in preindustrial times, plant N demand has increased more than supply.
Climate warming accelerates carbon release from foliar litter—A global synthesis
Authors: Chen Zihao , Ni Xiangyin , Patoine Guillaume , Peng Changhui , Yue Kai , Yuan Ji , Wu Qiuxia , Eisenhauer Nico , Guerra Carlos A. , Bol Roland , Wu Fuzhong , Wang G. Geoff 
Published: May 2024

AbstractWith over one‐third of terrestrial net primary productivity transferring to the litter layer annually, the carbon release from litter serves as a crucial valve in atmospheric carbon dioxide concentrations. However, few quantitative global projections of litter carbon release rate in response to climate change exist. Here, we combined a global foliar litter carbon release dataset (8973 samples) to generate spatially explicitly estimates of the response of their residence time (τ) to climate change. Results show a global mean litter carbon release rate () of 0.69 year−1 (ranging from 0.09–5.6 year−1). Under future climate scenarios, global mean τ is projected to decrease by a mean of 2.7% (SSP 1–2.6) and 5.9% (SSP 5–8.5) during 2071–2100 period. Locally, the alleviation of temperature and moisture restrictions corresponded to obvious decreases in τ in cold and arid regions, respectively. In contract, τ in tropical humid broadleaf forests increased by 4.6% under SSP 5–8.5. Our findings highlight the vegetation type as a powerful proxy for explaining global patterns in foliar litter carbon release rates and the role of climate conditions in predicting responses of carbon release to climate change. Our observation‐based estimates could refine carbon cycle parameterization, improving projections of carbon cycle–climate feedbacks.
VC ownership post‐IPO: When, why, and how do VCs exit?
Authors: Basnet Anup , Pukthuanthong Kuntara , Turtle Harry , Walker Thomas 
Published: May 2024

AbstractWe examine the evolution of lead venture capital firm (VC) ownership after their portfolio companies (PCs) are publicly listed. We find that, on average, lead VCs retain their shares for three years post‐IPO. Higher liquidity pressure and better stock market performance lead to faster VC exits, while higher VC reputation, better VC monitoring, and higher quality PCs lead to slower exits. VCs mostly use sales in the open market, share distributions, and mergers and acquisitions to divest their shares. Higher liquidity pressure incentivizes VCs to use majority share distributions, while better stock market performance increases their preference for continuous sales.
Hydronidone induces apoptosis in activated hepatic stellate cells through endoplasmic reticulum stress‐associated mitochondrial apoptotic pathway
Authors: Sun Zhongshang , Guo Yuecheng , Xu Xianjun , Zhou Cui , Luo Xin , Shen Zhenyang , Shen Bo , Wang Junjun , Lu Jingyi , Zhang Qingqing , Shen Feifei , Yu Lou , Ye Yanping , Zhang Ling , Luo Ying , Qu Ying , Cai Xiaobo , Dong Hui , Lu Lungen 
Published: May 2024

AbstractBackground and AimHydronidone (HDD) is a novel pirfenidone derivative developed initially to reduce hepatotoxicity. Our previous studies in animals and humans have demonstrated that HDD treatment effectively attenuates liver fibrosis, yet the underlying mechanism remains unclear. This study aimed to investigate whether HDD exerts its anti‐fibrotic effect by inducing apoptosis in activated hepatic stellate cells (aHSCs) through the endoplasmic reticulum stress (ERS)‐associated mitochondrial apoptotic pathway.MethodsThe carbon tetrachloride (CCl4)‐ and 3,5‐diethoxycarbonyl‐1,4‐dihydrocollidine (DDC)‐induced liver fibrosis models were used for in vivo studies. In vitro studies were conducted using the human hepatic stellate cell line LX‐2. The apoptotic effect of HDD on aHSCs was examined using TUNEL and flow cytometry assays. The small interfering RNA (siRNA) technique was employed to downregulate the expression of interest genes.ResultsHDD treatment significantly promoted apoptosis in aHSCs in both the CCl4‐ and DDC‐induced liver fibrosis in mice and LX‐2 cells. Mechanistic studies revealed that HDD triggered ERS and subsequently activated the IRE1α‐ASK1‐JNK pathway. Furthermore, the influx of cytochrome c from the mitochondria into the cytoplasm was increased, leading to mitochondrial dysfunction and ultimately triggering apoptosis in aHSCs. Notably, inhibition of IRE1α or ASK1 by siRNA partially abrogated the pro‐apoptotic effect of HDD in aHSCs.ConclusionsThe findings of both in vivo and in vitro studies suggest that HDD induces apoptosis in aHSCs via the ERS‐associated mitochondrial apoptotic pathway, potentially contributing to the amelioration of liver fibrosis.
Phosphorylation of ZmAL14 by ZmSnRK2.2 regulates drought resistance through derepressing ZmROP8 expression
Authors: Wang Yalin , Cheng Jinkui , Guo Yazhen , Li Zhen , Yang Shuhua , Wang Yu , Gong Zhizhong 
Published: May 2024

ABSTRACTDrought stress has negative effects on crop growth and production. Characterization of transcription factors that regulate the expression of drought‐responsive genes is critical for understanding the transcriptional regulatory networks in response to drought, which facilitates the improvement of crop drought tolerance. Here, we identified an Alfin‐like (AL) family gene ZmAL14 that negatively regulates drought resistance. Overexpression of ZmAL14 exhibits susceptibility to drought while mutation of ZmAL14 enhances drought resistance. An abscisic acid (ABA)‐activated protein kinase ZmSnRK2.2 interacts and phosphorylates ZmAL14 at T38 residue. Knockout of ZmSnRK2.2 gene decreases drought resistance of maize. A dehydration‐induced Rho‐like small guanosine triphosphatase gene ZmROP8 is directly targeted and repressed by ZmAL14. Phosphorylation of ZmAL14 by ZmSnRK2.2 prevents its binding to the ZmROP8 promoter, thereby releasing the repression of ZmROP8 transcription. Overexpression of ZmROP8 stimulates peroxidase activity and reduces hydrogen peroxide accumulation after drought treatment. Collectively, our study indicates that ZmAL14 is a negative regulator of drought resistance, which can be phosphorylated by ZmSnRK2.2 through the ABA signaling pathway, thus preventing its suppression on ZmROP8 transcription during drought stress response.
Shrubs exhibit competitive interactions with herbaceous plants and shape community assemblage and functional composition in the alpine western Himalaya
Authors: Ram Bittu , Chawla Amit 
Published: May 2024

AbstractQuestionsIn the alpine region of the Himalaya, shrubs play a vital role in maintaining the diversity and functional composition of associated herbaceous communities through competition–facilitation interactions under varying environmental stress conditions. In this study, we specified the following questions: (1) what is the mode of interaction between dominant shrubs and the associated herbaceous communities; (2) how do differences in resource availability between contrasting microhabitats mediate interactions between shrub and herbaceous communities; and (3) how do dominant shrubs influence the functional composition of herbaceous communities under the canopy as compared to ones in the open?Study areaAn alpine region of the western Himalaya, in India (32.24–33.15° N, 76.51–78.13° E).MethodsField studies were conducted to evaluate the canopy effects of alpine shrubs such as Caragana versicolor, Juniperus polycarpos and Rhododendron anthopogon on species richness, abundance and functional composition of coexisting herbaceous communities.ResultsThe dominant alpine shrubs of the western Himalaya exert competitive interactions with associated herbaceous communities and have low species richness and abundance under their canopies compared to open habitats. Further, contrary to expectations, competitive interactions were more prominent at higher elevations than at lower ones, especially for J. polycarpos and C. versicolor. Although the shrub undercanopies possessed richer soil nutrient pools, this did not contribute toward the facilitation of herbaceous species under the canopies. Moreover, herbaceous species under the canopies were found to exhibit resource‐acquisitive functional strategies, whereas those in the open were resource‐conservative.ConclusionsThe interaction between dominant alpine shrubs and the herbaceous community of the western Himalaya is competitive in nature, which influences species and functional composition and reorganizes herbaceous community assembly. Moreover, under future climate change scenarios the dominance of these shrubs will favour those herbaceous species that possess more competitive and resource‐acquisitive functional strategies.
Social networking sites' licensing terms: A cause of worry for users?
Authors: Mahapatra Phalguni , Sircar Anindya 
Published: May 2024

AbstractTerms of service (ToS) for social networking sites (SNS) like Instagram, Meta, X, and so on, is a clickwrap agreement that establishes a legal relationship between platform owners and users, yet probably it is the most overlooked legal agreement. The users of these sites often overlook the ToS while registering themselves on these sites and even if users (especially those with no legal background) are attempting to read them, it is difficult for them to understand because of the legal jargon. As a result, they end up signing away legal rights about which they are unaware. According to these sites' ToS, though the ownership of the user‐generated content is bestowed upon the user but the users grant to these sites “a non‐exclusive, royalty‐free, transferrable, sub‐licensable, worldwide license” and this license can be used “to host, use, distribute, modify, run, copy, publicly perform or display, translate and create derivative works of user's content.” These sites even bestow on themselves the right to modify the content which poses challenges to the right‐holders' moral rights. The fact that these platforms can sublicense the user's work creates complexities when a user intends to grant an exclusive license of his work. There is no clarity on the language of the terms like the manner of exploiting the user's content, what happens if the sublicensing is for a wrongful purpose? The problem magnifies as there is neither explicit indication about the duration of the license nor about the territorial extent. This would suggest that these sites can get a perpetual license on the content of the users. These SNS have consumers spread worldwide but in their ToS, they have forum selection clauses that list out the courts and districts in California. This means users will be discouraged to bring a copyright suit due to the lack of an option to file a claim in their home country. The US case Agence France Presse (AFP) v. Morel helps us conclude twofold mainly there is a hope that SNS will not take ToS to shield themselves from further use of the user's work and strengthen the idea that these platforms may choose to license to their partners. Further, in 2018, the Paris Tribunal declared most clauses of Twitter “null and void” due to the nature of the license and also, because it was not in compliance with French Intellectual Property Code. This gives a faint hope for a positive shift in the legal treatment of user‐generated content. Though these sites claim to retain the sublicensing right to run their sites smoothly but the licensing is very broad and carries the possibility of many usages of the content that too without paying compensation to the user. Therefore, this paper aims to highlight and give insight into the unfair licensing terms of the most often used social networking sites and its implications.
Sources and determinants of free sugars intake by 5‐year‐old Australian children in the SMILE cohort
Authors: Ivaturi Anupama , Do Loc Giang , Giles Lynne , Devenish Gemma , Scott Jane A. , Ha Diep H. , Golley Rebecca , Bell Lucy K. , Moynihan Paula J. 
Published: May 2024

AbstractReducing free sugars intake is important for the prevention of dental caries and obesity in children. The study aimed to determine the amount and sources of free sugars known to contribute to dental caries, and identify sociodemographic determinants of intake by children aged 5 years in Australia. Cross‐sectional analysis of dietary data from a cohort study, collected using a customized food frequency questionnaire were used to calculate free sugars intake as grams/day and percentage contribution to Estimated Energy Requirement (EER). The percent contribution of food sources to free sugars intake was derived. Sociodemographic determinants of achieving intakes within WHO thresholds (i.e., <5% and <10% Energy were explored with multinomial logistic regression. Complete data were available for 641 children (347 boys, 294 girls). Median (IQR) free sugars intake (g/day) was 31.6 (21.3–47.6) in boys and 28.1 (19.6–47.9) in girls. The median (IQR) percentage contribution to EER was 7.9 (5.4–12.7); 21% and 42% of children had intakes <5% EER and between 5% and <10%, respectively. The main sources of free sugars were: (1) Cakes, Biscuits and Cereal Bars; (2) Sweetened Milk Products (predominantly yoghurts) and (3) Desserts. Maternal university education, single‐parent household, and maternal place of birth being Australia or New Zealand were associated with free sugars intake <5% EER. In conclusion, less than a quarter of 5‐year‐old children in the SMILE cohort achieved the WHO recommendations to limit free sugars to <5% EER. Strategies to lower free sugars intake could target priority populations such migrants, populations with lower levels of education or health literacy and identify areas for intervention in the wider food environments that children are exposed to.
Maternal misperception of under‐five children weight status and associated factors: A cross‐sectional study
Authors: Sarker Tanmoy , Ahmed Sabrina , Rahman Sakib , Chakraborty Barnali 
Published: May 2024

AbstractMaternal perceptions, about the nutritional status of their children, may have implications for timely health care seeking and feeding behaviour. Shreds of evidence are limited in this area that assessed maternal perception about the nutritional status of the children in the context of Bangladesh. The objective of this study is to assess the differences in mothers' perceptions about their children's nutritional status who are less than 5 years of age and their associated factors in the context of urban slum areas. A cross‐sectional study (Quantitative approach) was conducted in two of the largest urban slums of Dhaka city. A total of 437 mother–children dyads were included in the final analysis. The outcome of interest was the mother's ‘varied perceptions’ regarding the nutritional status of their children under five. ‘Varied perception’ refers to the difference between a mother's subjective assessment of her child's nutritional condition and the anthropometrically measured nutritional status. The prevalence of mother's varied perceptions was around 46%, and among them, 37% of mothers underestimated and 9% overestimated their child's nutritional status. ‘The child doesn't want to eat’ (AOR = 3.69; 95% CI: 2.34–5.85; p < 0.001) and ‘Feels light when carried’ (AOR = 3.39; 95% CI: 1.69–6.81; p = 0.001) were the significant reasons behind the mother's perception about the nutritional status of their under‐five children. About one in two mothers' perceptions about their children varied from the anthropometry‐derived status. Various research findings indicate that providing health education on proper feeding practices for children, along with anthropometric measurements, can assist mothers in enhancing their understanding and assessing their child's nutritional status with greater accuracy. Further qualitative research may be instrumental in getting deeper insights into maternal perception to develop context‐specific interventions.