bluuebunny/crossref_metadata_2025_split huggingface dataset:
The impact of pre-, pro- and synbiotics supplementation in colorectal cancer treatment: a systematic review
Authors: Moreira Mariana Melo , Carriço Marta , Capelas Manuel Luís , Pimenta Nuno , Santos Teresa , Ganhão-Arranhado Susana , Mäkitie Antti , Ravasco Paula 
Published: May 2024

IntroductionThe effectiveness of the supplementation of prebiotics, probiotics and synbiotics as a therapeutic approach in colorectal cancer (CRC) remains unclear. The aim of this systematic review is to critically examine the current scientific evidence on the impact of modulating the microbiota, through the use of prebiotics, probiotics and synbiotics, in patients diagnosed with CRC undergoing treatment, to determine the potential therapeutic use of this approach.MethodsThis systematic review was made according to the PRISMA 2020 guidelines. Inclusion criteria were randomized controlled trials (RCT) comparing the impact of pre-, pro-, or synbiotic supplementation with placebo or standard care in patients with CRC undergoing treatment. Exclusion criteria were non-human studies, non-RCTs, and studies in languages other than English or Portuguese. Six databases were consulted, namely, Cochrane Library, Pubmed, Scopus, Cinahl, MedicLatina and Web of Science until May of 2023. RAYYAN software was used to manage the search results and risk of bias was assessed according to the guidelines of the Cochrane Collaboration using the Rob 2.0 tool.ResultsTwenty-four RCTs met the inclusion criteria and were included in this review. Administration of pre-, pro-, or synbiotics improved surgical outcomes such as the incidence of infectious and non-infectious postoperative complications, return to normal gut function, hospital length of stay, and antibiotic usage. The supplementation of these microorganisms also alleviated some symptoms from chemotherapy and radiotherapy, mainly diarrhea. Evidence on the best approach in terms of types of strains, dosage and duration of intervention is still scarce.ConclusionsPre-, pro-, and synbiotics supplementation appears to be a beneficial therapeutic approach in CRC treatment to improve surgical outcomes and to alleviate side-effects such as treatment toxicity. More RCTs with larger sample sizes and less heterogeneity are needed to confirm these potential benefits and to determine the best strains, dosage, and duration of administration in each situation.Systematic review registrationhttps://www.crd.york.ac.uk/prospero, identifier CRD42023413958.
Histopathological predictors of lymph node metastasis in oral cavity squamous cell carcinoma: a systematic review and meta-analysis
Authors: Alqutub Sadiq , Alqutub Abdulsalam , Bakhshwin Ahmed , Mofti Zainab , Alqutub Sulafa , Alkhamesi Ameera A. , Nujoom Mohammed A. , Rammal Almoaidbellah , Merdad Mazin , Marzouki Hani Z. 
Published: May 2024

ObjectivesLymph node metastasis (LNM) is the most significant parameter affecting overall survival in patients with oral cavity squamous cell carcinomas (OCSCC). Elective neck dissection (END) is the standard of care in the early management of OCSCC with a depth of invasion (DOI) greater than 2-4 mm. However, most patients show no LNM in the final pathologic report, indicating overtreatment. Thus, more detailed indicators are needed to predict LNM in patients with OCSCC. In this study, we critically evaluate the existing literature about the risk of different histological parameters in estimating LNM.MethodsA systematic review was conducted using PRISMA guidelines. PubMed, Web of Science, Cochrane, and Scopus were searched from inception to December 2023 to collect all relevant studies. Eligibility screening of records was performed, and data extraction from the selected studies was carried out independently. Inclusion in our systematic review necessitated the following prerequisites: Involvement of patients diagnosed with OCSCC, and examination of histological parameters related to lymph node metastasis in these studies. Exclusion criteria included animal studies, non-English articles, non-availability of full text, and unpublished data.ResultsWe included 217 studies in our systematic review, of which 142 were eligible for the meta-analysis. DOI exceeding 4 mm exhibited higher risk for LNM [Risk ratio (RR) 2.18 (1.91-2.48), p&lt;0.00001], as did perineural invasion (PNI) [RR 2.04 (1.77-2.34), p&lt;0.00001], poorly differentiated tumors [RR 1.97 (1.61-2.42), p&lt;0.00001], lymphovascular invasion (LVI) [RR 2.43 (2.12-2.78), p&lt;0.00001], groups and single pattern of invasion [RR 2.47 (2.11-2.89), p&lt;0.00001], high tumor budding [RR 2.65 (1.99-3.52), p&lt;0.00001], tumor size over 4 cm [RR 1.76 (1.43-2.18), p&lt;0.00001], tumor thickness beyond 4 mm [RR 2.72 (1.91-3.87), p&lt;0.00001], involved or close margin [RR 1.73 (1.29-2.33), p = 0.0003], and T3 and T4 disease [RR 1.98 (1.62-2.41), p &lt;0.00001].ConclusionOur results confirm the potential usefulness of many histopathological features in predicting LNM and highlight the promising results of others. Many of these parameters are not routinely incorporated into pathologic reports. Future studies must focus on applying these parameters to examine their validity in predicting the need for elective neck treatment.
The effect of vitamin D deficiency during pregnancy on adverse birth outcomes in neonates: a systematic review and meta-analysis
Authors: You Zhiying , Mei Hua , Zhang Yayu , Song Dan , Zhang Yanbo , Liu Chunli 
Published: May 2024

ObjectiveTo systematically evaluate the effect of vitamin D deficiency during pregnancy on neonatal adverse outcomes, such as preterm infants, low birth weight infants (LBWI), and small for gestational age (SGA) infants.MethodsA comprehensive literature search was conducted across multiple databases including PubMed, Embase, Cochrane Library, SinoMed, Wanfang Data Knowledge Service Platform, China National Knowledge Internet (CNKI), and VIP Chinese Science and Technology Journal Database (VIP). Following predefined inclusion and exclusion criteria, two researchers independently screened, extracted data, and assessed the quality of the included studies. Meta-analysis was performed using RevMan 5.4 and Stata 14 software to synthesize the findings.ResultsThis study incorporated 13 cohort studies from 8 different countries and regions, encompassing a total of 55,162 pregnant women, among whom 28,155 were identified as having vitamin D deficiency. The Newcastle-Ottawa Scale (NOS) score ranged from 7–9 points. Meta-analysis results indicated a higher incidence of LBWI (OR = 5.52, 95% CI = 1.31–23.22. P = 0.02) in the group of pregnant women with vitamin D deficiency compared to those with adequate levels. However, there was no statistically significant difference in the likelihood of premature birth (OR = 1.25, 95% CI = 0.78–1.99. P = 0.36) or SGA (OR = 1.47, 95% CI = 0.81–2.68. P = 0.21) among newborns born to mothers with vitamin D deficiency vs. those with sufficient levels of vitamin D. Subgroup analysis based on the timing of maternal blood collection revealed that there was no statistically significant association between vitamin D levels during pregnancy and the incidence of preterm birth across all stages of pregnancy. Furthermore, vitamin D deficiency throughout the entire pregnancy was associated with an increased incidence of neonatal LBWI, whereas vitamin D levels during the first, second, and third trimesters did not demonstrate statistically differences on LBWI. Neonates born to mothers with vitamin D deficiency throughout pregnancy were found to have a higher likelihood of developing SGA. However, there was no statistically significant association between vitamin D levels and the development of SGA during the first and second trimesters.ConclusionsAdequate levels of vitamin D during pregnancy may decrease the incidence of LBWI, although further research is needed to determine its impact on the occurrence of preterm birth and SGA.Systematic Review Registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42024535950, Identifier: (CRD42024535950).
Classification of field wheat varieties based on a lightweight G-PPW-VGG11 model
Authors: Pan Yu , Yu Xun , Dong Jihua , Zhao Yonghang , Li Shuanming , Jin Xiuliang 
Published: May 2024

IntroductionIn agriculture, especially wheat cultivation, farmers often use multi-variety planting strategies to reduce monoculture-related harvest risks. However, the subtle morphological differences among wheat varieties make accurate discrimination technically challenging. Traditional variety classification methods, reliant on expert knowledge, are inefficient for modern intelligent agricultural management. Numerous existing classification models are computationally complex, memory-intensive, and difficult to deploy on mobile devices effectively. This study introduces G-PPW-VGG11, an innovative lightweight convolutional neural network model, to address these issues.MethodsG-PPW-VGG11 ingeniously combines partial convolution (PConv) and partially mixed depthwise separable convolution (PMConv), reducing computational complexity and feature redundancy. Simultaneously, incorporating ECANet, an efficient channel attention mechanism, enables precise leaf information capture and effective background noise suppression. Additionally, G-PPW-VGG11 replaces traditional VGG11’s fully connected layers with two pointwise convolutional layers and a global average pooling layer, significantly reducing memory footprint and enhancing nonlinear expressiveness and training efficiency.ResultsRigorous testing showed G-PPW-VGG11's superior performance, with an impressive 93.52% classification accuracy and only 1.79MB memory usage. Compared to VGG11, G-PPW-VGG11 showed a 5.89% increase in accuracy, 35.44% faster inference, and a 99.64% reduction in memory usage. G-PPW-VGG11 also surpasses traditional lightweight networks in classification accuracy and inference speed. Notably, G-PPW-VGG11 was successfully deployed on Android and its performance evaluated in real-world settings. The results showed an 84.67% classification accuracy with an average time of 291.04ms per image.DiscussionThis validates the model's feasibility for practical agricultural wheat variety classification, establishing a foundation for intelligent management. For future research, the trained model and complete dataset are made publicly available.
Animal-assisted interventions for military families: a systematic review
Authors: Nieforth Leanne O. , Leighton Sarah C. 
Published: May 2024

IntroductionThe incorporation of animals into interventions focused on military families is a relatively new concept. Though animal-assisted interventions (AAIs) have been studied in the context of military veterans, few studies incorporate members of the military family or focus on the family members’ experiences.MethodsThis systematic review investigates the effects of AAIs on the wellbeing of military family members beyond the veteran themselves through three aims: (1) by describing the characteristics of AAIs for military family members, (2) by evaluating the quality of the methodology present within the current literature, and (3) by identifying key concepts and knowledge gaps within the findings reported to date.ResultsA total of nine articles met the criteria to be included in the review. Though the inclusion criteria and search terms included all types of animal-assisted interventions, the only interventions represented were service dogs (n = 4) and equine-assisted services (n = 5).DiscussionFindings suggest AAIs could be beneficial in areas such as communication, relational bonds, and psychosocial well-being. Though additional research is necessary, AAIs may be an effective complementary intervention for military families.
The impact of a mixed reality technology-driven health enhancing physical activity program among community-dwelling older adults: a study protocol
Authors: Dino Michael Joseph S. , Dion Kenneth W. , Abadir Peter M. , Budhathoki Chakra , Huang Chien-Ming , Padula William V. , Himmelfarb Cheryl R. Dennison , Davidson Patricia M. 
Published: May 2024

BackgroundPhysical inactivity and a sedentary lifestyle among community-dwelling older adults poses a greater risk for progressive physical and cognitive decline. Mixed reality technology-driven health enhancing physical activities such as the use of virtual coaches provide an emerging and promising solution to support healthy lifestyle, but the impact has not been clearly understood.Methods and analysisAn observational explanatory sequential mixed-method research design was conceptualized to examine the potential impact of a user-preferred mixed reality technology-driven health enhancing physical activity program directed toward purposively selected community-dwelling older adults in two senior centers in the Philippines. Quantitative components of the study will be done through a discreet choice experiment and a quasi-experimental study. A total of 128, or 64 older adults in each center, will be recruited via posters at community senior centers who will undergo additional screening or health records review by a certified gerontologist to ensure safety and proper fit. Treatments (live coaching with video-based exercise and mixed reality technology-driven exercise) will be assigned to each of the two senior center sites for the quasi-experiment. The participants from the experimental group shall be involved in the discreet choice experiment, modeling, and usability evaluations. Finally, a qualitative sample of participants (n = 6) as key informants shall be obtained from the experimental group using purposive selection.DiscussionThis study protocol will examine the health impact of a promising mixed reality program in health promotion among older adults. The study utilizes a human-centered mixed method research design in technology development and evaluation in the context of developing nations.Clinical trial registration: ClinicalTrials.gov, identifier NCT06136468.
Development of a market-driven training model for rural women in Iran by using a qualitative paradigm
Authors: Allahmoradi Zeynab , Hosseini Seyed Jamal Farajallah , Lashgarara Farhad , Moghaddasi Reza 
Published: May 2024

ProblemTraining programs aimed at empowering rural women in Kurdistan, Iran, have fallen short of their goals. This study offers a unique understanding of how contextual factors and cultural nuances impact the effectiveness of market-driven training programs for rural women in Kurdistan.ObjectivesThis study explored factors affecting the effectiveness of market-driven training programs for rural women in Kurdistan.MethodsA qualitative approach using grounded theory methodology was employed. Interviews were conducted with 23 key informants, including rural women and experts. Data analysis with MAXQDA software identified seven categories and a core category related to market-driven training.FindingsThe study revealed that successful implementation of market-driven training hinges on various external and internal factors. These include access to markets, facilities, and funding, along with effective monitoring of business activities. Additionally, rural community culture and the purchasing power of women were identified as key intervening conditions impacting program success. When implemented effectively, market-driven training programs have the potential to empower women, reduce rural migration, and improve product quality.RecommendationsThe research suggests that future training programs should adopt a multi-dimensional approach that addresses the identified factors to achieve sustainable positive outcomes for rural women in Kurdistan.
Novel polymorphisms and genetic studies of the shadow of prion protein gene (SPRN) in pheasants
Authors: Choi Da-In , Zayed Mohammed , Kim Yong-Chan , Jeong Byung-Hoon 
Published: May 2024

BackgroundPrion diseases in mammals are caused by the structural conversion of the natural prion protein (PrPC) to a pathogenic isoform, the “scrapie form of prion protein (PrPSc).” Several studies reported that the shadow of prion protein (Sho), encoded by the shadow of prion protein gene (SPRN), is involved in prion disease development by accelerating the conformational conversion of PrPC to PrPSc. Until now, genetic polymorphisms of the SPRN gene and the protein structure of Sho related to fragility to prion disease have not been investigated in pheasants, which are a species of poultry.MethodsHere, we identified the SPRN gene sequence by polymerase chain reaction (PCR) and compared the SPRN gene and Sho protein sequences among various prion disease-susceptible and -resistant species to identify the distinctive genetic features of pheasant Sho using Clustal Omega. In addition, we investigated genetic polymorphisms of the SPRN gene in pheasants and analyzed genotype, allele, and haplotype frequencies, as well as linkage disequilibrium among the genetic polymorphisms. Furthermore, we used in silico programs, namely Mutpred2, MUpro and AMYCO, to investigate the effect of non-synonymous single nucleotide polymorphisms (SNPs). Finally, the predicted secondary and tertiary structures of Sho proteins from various species were analyzed by Alphafold2.ResultsIn the present study, we reported pheasant SPRN gene sequences for the first time and identified a total of 14 novel SNPs, including 7 non-synonymous and 4 synonymous SNPs. In addition, the pheasant Sho protein sequence showed 100% identity with the chicken Sho protein sequence. Furthermore, amino acid substitutions were predicted to affect the hydrogen bond distribution in the 3D structure of the pheasant Sho protein.ConclusionTo the best of our knowledge, this is the first report of the genetic and structural features of the pheasant SPRN gene.
Case report: Initial atypical skeletal symptoms and dental anomalies as first signs of Gardner syndrome: the importance of genetic analysis in the early diagnosis
Authors: Antal Gréta , Zsigmond Anna , Till Ágnes , Orsi Eniko , Szanto Ildiko , Büki Gergely , Kereskai László , Herbert Zsuzsanna , Hadzsiev Kinga , Bene Judit 
Published: May 2024

Background: Gardner syndrome is a rare genetic cancer predisposition disorder characterized by intestinal polyposis, multiple osteomas, and soft and hard tissue tumors. Dental anomalies are present in approximately 30%–70% of patients with Gardner syndrome and can be discovered during routine dental examinations. However, sometimes the diagnosis is challenging due to the high clinical variability and incomplete clinical picture. Herein, we report a family with various dental and bone anomalies, in which the definitive diagnosis was established with the help of a comprehensive genetic analysis based on state-of-the-art next-generation sequencing technology.Case presentation: A 17-year-old female index patient presented with dental (caries, impacted, retained and anteriorly located teeth) and atypical bone anomalies not resembling Gardner syndrome. She was first referred to our Genetic Counselling Unit at the age of 11 due to an atypical bone abnormality identified by a panoramic X-ray. Tooth 3.6 was surgically removed and the histopathology report revealed a Paget’s disease-like bone metabolic disorder with mixed osteoblastic and osteoclastic activity of the mandible. A small lumbar subcutaneous tumor was discovered by physical examination. Ultrasound examination of the tumor raised the possibility of a soft tissue propagation of chondromatosis. Her sister, 2 years younger at the age of 14, had some benign tumors (multiple exostoses, odontomas, epidermoid cysts) and impacted teeth. Their mother had also skeletal symptoms. Her lower teeth did not develop, the 9th-10th ribs were fused, and she complained of intermittent jaw pain. A cranial CT scan showed fibrous dysplasia on the cranial bones. Whole exome sequencing identified a heterozygous pathogenic nonsense mutation (c.4700C&gt;G; p.Ser1567*) in the APC gene in the index patient’s DNA. Targeted sequencing revealed the same variant in the DNA of the other affected family members (the sister and the mother).Conclusion: Early diagnosis of this rare, genetically determined syndrome is very important, because of the potentially high malignant transformation of intestinal polyps. Dentists should be familiar with the typical maxillofacial features of this disorder, to be able to refer patients to genetic counseling. Dental anomalies often precede the intestinal polyposis and facilitate the early diagnosis, thereby increasing the patients’ chances of survival. Genetic analysis may be necessary in patients with atypical phenotypic signs.
Study and Modelling of the Impact of June 2015 Geomagnetic Storms on the Brazilian Ionosphere
Authors: Afolabi Oladayo O. , Candido Claudia Maria Nicoli , Becker-Guedes Fabio , Amory-Mazaudier Christine 
Published: May 2024

This study investigated the impact of the June 2015 geomagnetic storms on the Brazilian equatorial and low-latitude ionosphere by analyzing various data sources, including solar wind parameters from the advanced compositional explorer satellite (ACE), global positioning satellite vertical total electron content (GPS-VTEC), geomagnetic data, and validation of the SAMI2 model-VTEC with GPS-VTEC. The effect of geomagnetic disturbances on the Brazilian longitudinal sector was examined by applying multiresolution analysis (MRA) of the maximum overlap discrete wavelet transform (MODWT) to isolate the diurnal component of the disturbance dynamo (Ddyn), DP2 current fluctuations from the ionospheric electric current disturbance (Diono), and semblance cross-correlation wavelet analysis for local phase comparison between the Sq and Diono currents. Our findings revealed that the significant fluctuations in DP2 at the Brazilian equatorial stations (Belem, dip lat: −0.47° and Alta Floresta, dip lat: −3.75°) were influenced by IMF Bz oscillations; the equatorial electrojet also fluctuated in tandem with the DP2 currents, and dayside reconnection generated the field-aligned current that drove the DP2 current system. The short-lived positive ionospheric storm during the main phase on 22 June in the Southern Hemisphere in the Brazilian sector was caused by the interplay between the eastward prompt penetration of the magnetospheric convection electric field and the westward disturbance dynamo electric field. The negative ionospheric storms that occurred during the recovery phase from 23 to 29 June 2015, were attributed to the westward disturbance dynamo electric field, which caused the downward E × B drift of the plasma to a lower height with a high recombination rate. The comparison between the SAMI2 model-VTEC and GPS-VTEC indicates that the SAMI2 model underestimated the VTEC within magnetic latitudes of −9° to −24° in the Brazilian longitudinal sector from 6 to 17 June 2015. However, it demonstrated satisfactory agreement with the GPS-VTEC within magnetic latitudes of −9° to 10° from 8 to 15 June 2015. Conversely, the SAMI2 model overestimated the VTEC between ±10° magnetic latitudes from 16 to 28 June 2015. The most substantial root mean square error (RMSE) values, notably 10.30 and 5.48 TECU, were recorded on 22 and 23 June 2015, coinciding with periods of intense geomagnetic disturbance.