bluuebunny/crossref_metadata_2025_split huggingface dataset:
Detection of CLCF1 protein expression by flow cytometry
Authors: Laplante Véronique , Rousseau Marine , Lombard-Vadnais Félix , Nadeau Ulysse , Nazha Agathe , Schmouth Jean-François , Sharma Mukut , Lesage Sylvie , Gauchat Jean-François , Pasquin Sarah 
Published: June 2024

AbstractCardiotrophin-like cytokine factor 1 (CLCF1) is an IL-6 family cytokine with neurotrophic and immuno-modulating functions. CLCF1 mRNA has been detected in primary and secondary lymphoid organs, and up-regulation of CLCF1 mRNA levels has been associated with the T helper (Th) 17 polarization. However, information regarding CLCF1 expression by immune cells at the protein level remains scarce. We have developed a methodology that uses a monoclonal antibody (mAb) directed against CLCF1 for the detection of human and mouse CLCF1 by flow cytometry. We have successfully detected CLCF1 protein expression in cells from the mouse pro-B cell line Ba/F3 that were transduced with CLCF1 cDNA. Interestingly, we found that the anti-CLCF1 mAb inhibits CLCF1 biological activity in vitro by binding to an epitope that encompasses site III of the cytokine. Moreover, we have detected CLCF1 expression in mouse splenic T cells, as well as in vitro differentiated Th1 cells. The specificity of the fluorescence signal was demonstrated using Clcf1-deficient lymphocytes generated using a conditional knock-out mouse model. The detection of CLCF1 protein by flow cytometry will be a valuable tool to study CLCF1 expression during normal and pathological immune responses.
Effect of nutritional status on occurrence of pneumonia after traumatic cervical spinal cord injury
Authors: Hayashi Tetsuo , Fujiwara Yuichi , Irie Momo , Masuda Muneaki , Sakai Hiroaki , Kobayashi Hiromitsu , Kawano Osamu , Maeda Takeshi 
Published: June 2024

AbstractPneumonia after cervical spinal cord injury (CSCI) is a common and serious complication; however, its nutrition-related etiology has not yet been elucidated. This study aimed to elucidate the effects of nutritional factors on pneumonia after CSCI. Patients with acute traumatic CSCI who were admitted within 3 days after injury and followed up for at least 3 months were retrospectively examined. Occurrence of pneumonia, nutritional status, severity of dysphagia, vital capacity, use of respirators, and motor scores for paralysis were evaluated. Of 182 patients included in this study, 33 (18%) developed pneumonia. Multiple logistic regression analysis revealed that low nutritional status, severe paralysis, and low vital capacity were significant risk factors for pneumonia. The severity of paralysis, respiratory dysfunction, and poor nutritional status can affect the occurrence of pneumonia after CSCI. In addition to respiratory management, nutritional assessment and intervention may play key roles in preventing pneumonia associated with spinal cord injury-induced immune depression. Nutritional care should be provided as soon as possible when the nutritional status of a patient worsens after an injury.
Finding influential nodes in complex networks based on Kullback–Leibler model within the neighborhood
Authors: Wang Guan , Sun Zejun , Wang Tianqin , Li Yuanzhe , Hu Haifeng 
Published: June 2024

AbstractAs a research hot topic in the field of network security, the implementation of machine learning, such as federated learning, involves information interactions among a large number of distributed network devices. If we regard these distributed network devices and connection relationships as a complex network, we can identify the influential nodes to find the crucial points for optimizing the imbalance of the reliability of devices in federated learning system. This paper will analyze the advantages and disadvantages of existing algorithms for identifying influential nodes in complex networks, and propose a method from the perspective of information dissemination for finding influential nodes based on Kullback–Leibler divergence model within the neighborhood (KLN). Firstly, the KLN algorithm removes a node to simulate the scenario of node failure in the information dissemination process. Secondly, KLN evaluates the loss of information entropy within the neighborhood after node removal by establishing the KL divergence model. Finally, it assesses the damage influence of the removed node by integrating the network attributes and KL divergence model, thus achieving the evaluation of node importance. To validate the performance of KLN, this paper conducts an analysis and comparison of its results with those of 11 other algorithms on 10 networks, using SIR model as a reference. Additionally, a case study was undertaken on a real epidemic propagation network, leading to the proposal of management and control strategies for daily protection based on the influential nodes. The experimental results indicate that KLN effectively evaluates the importance of the removed node using KL model within the neighborhood, and demonstrate better accuracy and applicability across networks of different scales.
Natural allelic variation confers diversity in the regulation of flag leaf traits in wheat
Authors: Schierenbeck Matías , Alqudah Ahmad Mohammad , Thabet Samar Gamal , Avogadro Evangelina Gabriela , Dietz Juan Ignacio , Simón María Rosa , Börner Andreas 
Published: June 2024

AbstractFlag leaf (FL) dimension has been reported as a key ecophysiological aspect for boosting grain yield in wheat. A worldwide winter wheat panel consisting of 261 accessions was tested to examine the phenotypical variation and identify quantitative trait nucleotides (QTNs) with candidate genes influencing FL morphology. To this end, four FL traits were evaluated during the early milk stage under two growing seasons at the Leibniz Institute of Plant Genetics and Crop Plant Research. The results showed that all leaf traits (Flag leaf length, width, area, and length/width ratio) were significantly influenced by the environments, genotypes, and environments × genotypes interactions. Then, a genome-wide association analysis was performed using 17,093 SNPs that showed 10 novel QTNs that potentially play a role in modulating FL morphology in at least two environments. Further analysis revealed 8 high-confidence candidate genes likely involved in these traits and showing high expression values from flag leaf expansion until its senescence and also during grain development. An important QTN (wsnp_RFL_Contig2177_1500201) was associated with FL width and located inside TraesCS3B02G047300 at chromosome 3B. This gene encodes a major facilitator, sugar transporter-like, and showed the highest expression values among the candidate genes reported, suggesting their positive role in controlling flag leaf and potentially being involved in photosynthetic assimilation. Our study suggests that the detection of novel marker-trait associations and the subsequent elucidation of the genetic mechanism influencing FL morphology would be of interest for improving plant architecture, light capture, and photosynthetic efficiency during grain development.
Topological optimization and fatigue life prediction of a single pad externally adjustable fluid film bearing
Authors: Kamat Harishkumar , Pai Anand , Vernekar Navaneeth Krishna , Kini Chandrakant R. , Shenoy Satish B. 
Published: June 2024

AbstractThis work focuses on the prediction and comparison of the fatigue life of topologically optimized pads in an externally adjustable fluid film (EAFF) bearing. It integrates one-way/two-way fluid–structure interaction analysis, topological optimization (TO), and design modifications of the pad of an externally adjustable fluid film bearing. The major goal is to create an optimum pad design that minimizes weight and maintains structural integrity, and then to predict and compare the fatigue life of these alternative designs. The outcomes of the present study are as follows: (i) Two-way FSI results show a decrease of 65.64% in hydrodynamic fluid film pressure when compared to one-way FSI results because they take into account modifications in the fluid region's geometry caused by pad deformation; (ii) even though the maximum pad deformation in optimized pad geometry (Type-4) resulting from oil film pressure is relatively small (0.0036551 mm), the influence of pad deformation on the fluid domain due to hydrodynamic fluid film pressure cannot be understated; and (iii) when comparing the TO technique's results with fatigue life results, four elongated holes in the radial direction (Type-4) are most appropriate.
Residues of atrazine and diuron in rice straw, soils, and air post herbicide-contaminated straw biomass burning
Authors: Lamnoi Suteekan , Boonupara Thirasant , Sumitsawan Sulak , Vongruang Patipat , Prapamontol Tippawan , Udomkun Patchimaporn , Kaewlom Puangrat 
Published: June 2024

AbstractThis study investigates the environmental impact of burning herbicide-contaminated biomass, focusing on atrazine (ATZ) and diuron (DIU) sprayed on rice straw prior to burning. Samples of soil, biomass residues, total suspended particulate (TSP), particulate matter with an aerodynamic diameter ≤ 10 µm (PM10), and aerosols were collected and analyzed. Soil analysis before and after burning contaminated biomass showed significant changes, with 2,4-dichlorophenoxyacetic acid (2,4-D) initially constituting 79.2% and decreasing by 3.3 times post-burning. Atrazine-desethyl, sebuthylazine, and terbuthylazine were detected post-burning. In raw rice straw biomass, terbuthylazine dominated at 80.0%, but burning ATZ-contaminated biomass led to the detection of atrazine-desethyl and notable increases in sebuthylazine and terbuthylazine. Conversely, burning DIU-contaminated biomass resulted in a shift to 2,4-D dominance. Analysis of atmospheric components showed changes in TSP, PM10, and aerosol samples. Linuron in ambient TSP decreased by 1.6 times after burning ATZ-contaminated biomass, while atrazine increased by 2.9 times. Carcinogenic polycyclic aromatic hydrocarbons (PAHs), including benzo[a]anthracene (BaA), benzo[a]pyrene (BaP), and benzo[b]fluoranthene (BbF), increased by approximately 9.9 to 13.9 times after burning ATZ-contaminated biomass. In PM10, BaA and BaP concentrations increased by approximately 11.4 and 19.0 times, respectively, after burning ATZ-contaminated biomass. This study sheds light on the environmental risks posed by burning herbicide-contaminated biomass, emphasizing the need for sustainable agricultural practices and effective waste management. The findings underscore the importance of regulatory measures to mitigate environmental contamination and protect human health.
FOXO3a/PI3K/Akt pathway participates in the ROS- induced apoptosis triggered by α-ZEL and β-ZEL
Authors: Habrowska-Górczyńska Dominika Ewa , Kozieł Marta Justyna , Urbanek Kinga Anna , Kowalska Karolina , Piastowska-Ciesielska Agnieszka Wanda 
Published: June 2024

AbstractZearalenone (ZEN), an estrogenic mycotoxin, is one of the most common food and feed contaminants. Also, its metabolites α-zearalenol (α-ZEL) and β-zearalenol (β-ZEL) are considered to induce oxidative stress, however its effect in prostate cells is not known yet. Our previous observations showed that forehead box transcription factor 3a (FOXO3a) expression is modified in hormone- sensitive cells in the response to mycotoxins, similar to the phosphoinositide 3-kinase (PI3K)/ protein kinase B (Akt) pathway. Thus, this study evaluated the direct molecular effect of α-ZEL and β-ZEL in a dose of 30 µM in hormone-dependent human prostate cancer (PCa) cells with the focus of the involvement of FOXO3a and PI3K/Akt signaling pathway in that effect. We observed that both active metabolites of ZEN reduced cell viability, induced oxidative stress, cell cycle arrest and apoptosis in PCa cells. Furthermore, we observed that FOXO3a as well as PI3K/Akt signaling pathway participate in ZELs induced toxicity in PCa cells, indicating that this signaling pathway might be a regulator of mycotoxin-induced toxicity generally.
Extreme coastal El Niño events are tightly linked to the development of the Pacific Meridional Modes
Authors: Martinez-Villalobos Cristian , Dewitte Boris , Garreaud René D. , Loyola Leandra 
Published: June 2024

AbstractCoastal El Niño events—marine heatwaves instances in the far eastern Tropical Pacific during otherwise basin-scale neutral or cold conditions—can have severe societal impacts for countries along the west coast of South America, as exemplified by the 2017 and 2023 Peru-Ecuador floods. Due to the brevity of the observational record, it is not well understood whether these events are driven by local or large-scale processes. Here, to overcome this limitation we use a data-driven modeling approach to address their return period and forcing mechanisms. It is shown that extreme coastal El Niño events are a local manifestation in the eastern tropical Pacific of the constructive interactions of the Pacific Meridional Modes (PMM). Specifically, the North PMM yields a dipole-like anomaly SST pattern along the equator that favors its development, while the positive phase of the South PMM reinforces it. A smaller group of more moderate coastal events are remotely driven by zonal wind anomalies in the western tropical Pacific without the PMMs’ influence. The role of PMMs in the development of extreme coastal El Niño suggests that they may be more predictable than previously thought.
Transient brain activity dynamics discriminate levels of consciousness during anesthesia
Authors: Ensel Scott , Uhrig Lynn , Ozkirli Ayberk , Hoffner Guylaine , Tasserie Jordy , Dehaene Stanislas , Van De Ville Dimitri , Jarraya Béchir , Pirondini Elvira 
Published: June 2024

AbstractThe awake mammalian brain is functionally organized in terms of large-scale distributed networks that are constantly interacting. Loss of consciousness might disrupt this temporal organization leaving patients unresponsive. We hypothesize that characterizing brain activity in terms of transient events may provide a signature of consciousness. For this, we analyze temporal dynamics of spatiotemporally overlapping functional networks obtained from fMRI transient activity across different anesthetics and levels of anesthesia. We first show a striking homology in spatial organization of networks between monkeys and humans, indicating cross-species similarities in resting-state fMRI structure. We then track how network organization shifts under different anesthesia conditions in macaque monkeys. While the spatial aspect of the networks is preserved, their temporal dynamics are highly affected by anesthesia. Networks express for longer durations and co-activate in an anesthetic-specific configuration. Additionally, hierarchical brain organization is disrupted with a consciousness-level-signature role of the default mode network. In conclusion, large-scale brain network temporal dynamics capture differences in anesthetic-specific consciousness-level, paving the way towards a clinical translation of these cortical signature.
Super-resolution imaging of T lymphocyte activation reveals chromatin decondensation and disrupted nuclear envelope
Authors: Xu Jianquan , Sun Xuejiao , Chen Zhangguo , Ma Hongqiang , Liu Yang 
Published: June 2024

AbstractT lymphocyte activation plays a pivotal role in adaptive immune response and alters the spatial organization of nuclear architecture that subsequently impacts transcription activities. Here, using stochastic optical reconstruction microscopy (STORM), we observe dramatic de-condensation of chromatin and the disruption of nuclear envelope at a nanoscale resolution upon T lymphocyte activation. Super-resolution imaging reveals that such alterations in nuclear architecture are accompanied by the release of nuclear DNA into the cytoplasm, correlating with the degree of chromatin decompaction within the nucleus. The authors show that under the influence of metabolism, T lymphocyte activation de-condenses chromatin, disrupts the nuclear envelope, and releases DNA into the cytoplasm. Taken together, this result provides a direct, molecular-scale insight into the alteration in nuclear architecture. It suggests the release of nuclear DNA into the cytoplasm as a general consequence of chromatin decompaction after lymphocyte activation.
Interactive biocatalysis achieved by driving enzyme cascades inside a porous conducting material
Authors: Siritanaratkul Bhavin , Megarity Clare F. , Herold Ryan A. , Armstrong Fraser A. 
Published: June 2024

AbstractAn emerging concept and platform, the electrochemical Leaf (e-Leaf), offers a radical change in the way tandem (multi-step) catalysis by enzyme cascades is studied and exploited. The various enzymes are loaded into an electronically conducting porous material composed of metallic oxide nanoparticles, where they achieve high concentration and crowding – in the latter respect the environment resembles that found in living cells. By exploiting efficient electron tunneling between the nanoparticles and one of the enzymes, the e-Leaf enables the user to interact directly with complex networks, rendering simultaneous the abilities to energise, control and observe catalysis. Because dispersion of intermediates is physically suppressed, the output of the cascade – the rate of flow of chemical steps and information – is delivered in real time as electrical current. Myriad enzymes of all major classes now become effectively electroactive in a technology that offers scalability between micro-(analytical, multiplex) and macro-(synthesis) levels. This Perspective describes how the e-Leaf was discovered, the steps in its development so far, and the outlook for future research and applications.
Social and nonsocial synchrony are interrelated and romantically attractive
Authors: Cohen M. , Abargil M. , Ahissar M. , Atzil S. 
Published: June 2024

AbstractThe mechanisms of romantic bonding in humans are largely unknown. Recent research suggests that physiological synchrony between partners is associated with bonding. This study combines an experimental approach with a naturalistic dating setup to test whether the individual differences in social and nonsocial synchrony are interdependent, and linked to romantic attractiveness. In a preregistered online experiment with 144 participants, we discover that inducing physiological synchrony between an actor and an actress determines their attractiveness ratings by participants, indicating that synchrony can increase perceived attraction. In a lab-based naturalistic speed-dating experiment, we quantify in 48 participants the individual tendency for social physiological synchrony, nonsocial sensorimotor synchrony, and romantic attractiveness. We discover that the individual propensity to synchronize in social and nonsocial tasks is correlated. Some individuals synchronize better regardless of partners or tasks, and such Super Synchronizers are rated as more attractive. Altogether, this demonstrates that humans prefer romantic partners who can synchronize.
Multiscale perspectives for advancing sustainability in fiber reinforced ultra-high performance concrete
Authors: Wang Xing Quan , Chow Cheuk Lun , Lau Denvid 
Published: June 2024

AbstractUltra-high performance concrete (UHPC) integrates cutting-edge nano-additives, fibers and cementitious materials, which is a representative heterogeneous material and exhibits distinctive multi-scale structural characteristics. With remarkable durability and mechanical properties, lower embodied energy and diminished carbon emissions compared to conventional concrete, the application of UHPC aligns with the principles of sustainable development. To accelerate these advances, researchers of construction materials have incorporated a multiscale perspective into UHPC studies. From the perspective of sustainability, we evaluate the latest advances in the design, application and innovation of UHPC under multiscale perspective. Based on the fundamentals of fiber-reinforced UHPC, we discuss why and how could multiscale mechanics research, including analytical and modeling methods helps the advances of its sustainability, emphasizing the ecological considerations of UHPC in the practical applications. Then we summarize the challenges and perspectives in the design, production and construction of UHPC materials and structures. Based on advances in multiscale mechanics, a bright future can be envisioned where sustainable UHPC is applied globally, led by additive manufacturing and artificial intelligence.
From bone to nanoparticles: development of a novel generation of bone derived nanoparticles for image guided orthopedic regeneration
Authors: Stellpflug Austin , Walls Jacob , Hansen Christopher , Joshi Amit , Wang Bo 
Published: June 2024

This study developed a new type of bone-based nanoparticle (BPs) directly from the entire decellularized porcine bone, additionally encapsulating indocyanine green dye (ICG) for an in vivo monitoring capability.
Croissance cranio-faciale
Authors: Nakkache Maxime , Soyer Yves 
Published: June 2024

L’étude de la croissance cranio-faciale a toujours suscité des controverses parmi les auteurs. De nombreuses théories ont rendu compte de la croissance cranio-faciale. Son étude est d’une grande complexité et il est encore aujourd’hui difficile de l’aborder de façon exhaustive. La croissance physique correspond à l’augmentation des dimensions corporelles. Le développement correspond à la croissance et à la maturation des différentes fonctions et capacités fonctionnelles. Ces deux processus dépendent de facteurs génétiques, nutritionnels et environnementaux.
Le massif cranio-facial étant le support de fonctions essentielles, la connaissance des principes généraux et de l’évolution de la croissance physiologique est indispensable à la mise en place d’une thérapeutique efficace.
Approche biométrique : croissance faciale de l’enfant de moins de 7 ans, par le traitement d’image
Authors: Lalauze-Pol Roselyne , Jouen François 
Published: June 2024

Dans la littérature internationale, le taux de croissance de la face de l’enfant 7 ans reste largement méconnu. Classiquement seules les mesures radiographiques sont utilisées. Nous avons développé un logiciel autorisant le calcul des différentes dimensions faciales, en utilisant des photographies d’enfants de moins de 7 ans, à partir de 312 photographies. Les dimensions faciales sont calculées sous forme de ratio à partir de trente-sept repères faciaux adaptés au visage des enfants sélectionnés.
Les résultats indiquent que la hauteur de l’étage supérieur facial se réduit en fonction de l’âge et qu’il reste de taille plus réduite chez la fille. L’étage moyen du visage augmente précocement avec l’âge, avec la fonction respiratoire mais il est indépendant du sexe. L’étage facial inférieur augmente en fonction de l’âge, toujours avec un effet significatif du sexe. Les étages faciaux supérieur et moyen ont précocement des rapports similaires à ceux de l’adulte, mais l’étage inférieur reste de taille réduite, cependant son développement montre une évolution plus marquée chez les garçons que chez les filles.
Cette première quantification, non iatrogène, de la croissance faciale des enfants doit être appréciée avec des techniques plus performantes en trois dimensions, telle la stéréolitographie.