bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploring the regulatory role of FBXL19‐AS1 in triple‐negative breast cancer through the miR‐378a‐3p/OTUB2 axis
Authors: Guo Chenxu , Zhang Mingliang , Jin Xin , Zhu Chao , Qian Jun , Tao Min 
Published: May 2024

AbstractThe regulatory potential of long noncoding RNA (lncRNA) FBXL19‐AS1 has been highlighted in various cancers, but its effect on triple‐negative breast cancer (TNBC) remains unclear. Here, we aimed to elucidate the role of FBXL19‐AS1 in TNBC and its underlying mechanism. RT‐qPCR was employed to detect the expressions of FBXL19‐AS1 and miR‐378a‐3p in tissues and cells. Immunohistochemical staining and western blot were utilized to detect the expression levels of proteins. Cell activities were detected using flow cytometry, CCK‐8, and transwell assay. Dual‐luciferase reporter and RNA immunoprecipitation (RIP) assays were deployed to investigate interactions of different molecules. Protein–protein interaction (PPI) network, gene ontology (GO), and Kyoto encyclopedia of genes and genomes (KEGG) pathways were used to analyze the downstream pathway. In vivo xenograft model was conducted to detect the effect of FBXL19‐AS1 on tumor growth. FBXL19‐AS1 was overexpressed in TNBC tissues and cell lines compared with counterparts. FBXL19‐AS1 knockdown suppressed TNBC cell activities, whereas its overexpression exhibited the opposite effect. Mechanistically, FBXL19‐AS1 was found to interact with miR‐378a‐3p. Further analysis revealed that miR‐378a‐3p exerted tumor‐suppressive effects in TNBC cells. Additionally, miR‐378a‐3p targeted and downregulated the expression of ubiquitin aldehyde binding 2 (OTUB2), a deubiquitinase associated with TNBC progression. In vivo experiments substantiated the inhibitory effects of FBXL19‐AS1 knockdown on TNBC tumorigenesis, and a miR‐378a‐3p inhibitor partially rescued these effects. The downstream pathway of the miR‐378a‐3p/OTUB2 axis was explored, revealing connections with proteins involved in modifying other proteins, removing ubiquitin molecules, and influencing signaling pathways, including the Hippo signaling pathway. Western blot analysis confirmed changes in YAP and TAZ expression levels, indicating a potential regulatory network. In summary, FBXL19‐AS1 promotes exacerbation in TNBC by suppressing miR‐378a‐3p, leading to increased OTUB2 expression. The downstream mechanism may be related to the Hippo signaling pathway. These findings propose potential therapeutic targets for TNBC treatment.
Oligonucleotide‐Based Photoaffinity Probes: Chemical Tools and Applications for Protein Labeling
Authors: Saintomé Carole , Monfret Océane , Doisneau Gilles , Guianvarc'h Dominique 
Published: May 2024

AbstractA variety of proteins interact with DNA and RNA, including polymerases, histones, ribosomes, transcription factors, and repair enzymes. However, the transient non‐covalent nature of these interactions poses challenges for analysis. Introducing a covalent bond between proteins and DNA via photochemical activation of a photosensitive functional group introduced onto nucleic acids offers a means to stabilize these often weak interactions without significantly altering the binding interface. Consequently, photoactivatable oligonucleotides are powerful tools for investigating nucleic acid‐protein interactions involved in numerous biological and pathological processes. In this review, we provide a comprehensive overview of the chemical tools developed so far and the different strategies used for incorporating the most commonly used photoreactive reagents into oligonucleotide probes or nucleic acids. Furthermore, we illustrate their application with several examples including protein binding site mapping, identification of protein binding partners, and in cell studies.
Single coronary artery with aortic valve replacement followed by aortic root replacement due to endocarditis: A case report
Authors: Taoka Makoto , Sezai Akira , Ohba Masanao , Kitazumi Yoshiki , Hanamura Taisuke , Okumura Yasuo , Tanaka Masashi 
Published: May 2024

Key Clinical MessageA woman with a single coronary artery underwent aortic valve replacement due to aortic stenosis. Two years later, she developed an aortic annular abscess around the right coronary cusp and non‐coronary cusp. Significant adhesions to the right coronary artery (RCA) resulted from the abscess, making artery separation challenging, and raising concerns about potential future RCA stenosis. The patient subsequently underwent aortic root replacement and coronary artery bypass grafting. Utilizing a freestyle valve and a saphenous vein graft for the RCA. Following the procedure, the patient was discharged and has remained symptom‐free without any recurrence of infection for 2 years.
Gedunin modulates cellular growth and apoptosis in glioblastoma cell lines
Authors: Stouffer Michael , Wandling Elizabeth , Dickson Lindsay , Lin Stacy , Duan Huanyun , Powe Erika , Jean‐Louis Denise , Tiwari Amit K. , Amos Samson 
Published: May 2024

AbstractBackgroundGlioblastomas are characterized by aggressive behavior. Surgery, radiotherapy, and alkylating agents, including temozolomide are the most common treatment options for glioblastoma. Often, conventional therapies fail to treat these tumors since they develop drug resistance. There is a need for newer agents to combat this deadly tumor. Natural products such as gedunin have shown efficacy in several human diseases. A comprehensive study of gedunin, an heat shock protein (HSP)90 inhibitor, has not been thoroughly investigated in glioblastoma cell lines with different genetic modifications.AimsA key objective of this study was to determine how gedunin affects the biological and signaling mechanisms in glioblastoma cells, and to determine how those mechanisms affect the proliferation and apoptosis of glioblastoma cells.MethodsThe viability potentials of gedunin were tested using MTT, cell counts, and wound healing assays. Gedunin's effects on glioma cells were further validated using LDH and colony formation assays. In addition, we investigated the survival and apoptotic molecular signaling targets perturbed by gedunin using Western blot analysis and flow cytometry.ResultsOur results show that there was a reduction in cell viability and inhibition of wound healing in the cells tested. Western blot analysis of the gene expression data revealed genes such as EGFR and mTOR/Akt/NF kappa B to be associated with gedunin sensitivity. Gedunin treatment induced apoptosis by cleaving poly ADP‐ribose polymerase, activating caspases, and downregulating BCL‐xL. Based on these results, gedunin suppressed cell growth and HSP client proteins, resulting in apoptosis in glioblastoma cell lines.ConclusionOur data provide in vitro support for the anticancer activity of gedunin in glioma cells by downregulating cancer survival proteins.
Key players of immunosuppression in epithelial malignancies: Tumor‐infiltrating myeloid cells and γδ T cells
Authors: Tamuli Baishali , Sharma Sakshi , Patkar Meena , Biswas Subir 
Published: May 2024

AbstractBackgroundThe tumor microenvironment of solid tumors governs the differentiation of otherwise non‐immunosuppressive macrophages and gamma delta (γδ) T cells into strong immunosuppressors while promoting suppressive abilities of known immunosuppressors such as myeloid‐derived suppressor cells (MDSCs) upon infiltration into the tumor beds.Recent findingsIn epithelial malignancies, tumor‐associated macrophages (TAMs), precursor monocytic MDSCs (M‐MDSCs), and gamma delta (γδ) T cells often acquire strong immunosuppressive abilities that dampen spontaneous immune responses by tumor‐infiltrating T cells and B lymphocytes against cancer. Both M‐MDSCs and γδ T cells have been associated with worse prognosis for multiple epithelial cancers.ConclusionHere we discuss recent discoveries on how tumor‐associated macrophages and precursor M‐MDSCs as well as tumor associated‐γδ T cells acquire immunosuppressive abilities in the tumor beds, promote cancer metastasis, and perspectives on how possible novel interventions could restore the effective adaptive immune responses in epithelial cancers.
Impact of the Acetyl Group on Cysteine: A Study of N‐Acetyl‐Cysteine through Rotational Spectroscopy
Authors: Mato S. , Municio S. , Alonso J. L. , Alonso E. R. , León I. 
Published: May 2024

AbstractHerein, we report a spectroscopic study of N‐acetyl‐L‐cysteine, an important antioxidant drug, using Fourier‐transform microwave techniques and in isolated conditions. Two conformers are observed, where most stable structure adopts a cis disposition, and the second conformer has a lower abundance and adopts a trans disposition. The rotational constants and the barriers to methyl internal rotation are determined for each conformer, allowing a precise conformation identification. The results show that the cis form adopts an identical structure in the crystal, solution, and gas phases. Additionally, the structures are contrasted against those of cysteine.
Quantum Dynamics of O(3P)+HBr→OH+Br Reaction: Integral Cross Sections and Rate Constants and their Initial State Dependence
Authors: Lin Shi Ying 
Published: May 2024

AbstractThe integral cross sections (ICSs) and rate constants (RCs) for the title reaction are calculated by means of accurate quantum wave packet method employing the recent ab initio potential energy surface developed by Peterson and co‐workers. Hundreds of partial wave contributions (up to J=150) are calculated explicitly taking Coriolis coupling into account. The ICSs are found to increase monotonically with energy and their energy dependences are smooth except in the energy range below the potential energy barrier, where several resonance peaks are observed. The temperature dependences of the RCs obey in general the Arrhenius law. These behaviors manifest the dominance of abstract mechanism. Initial rotational state (ji) excitations are found to greatly enhance the reactivity starting from ji=4, while exhibiting a complicated dependence for ji<4. From Boltzmann average of the initial state specified RCs (for ji=0–15) we obtained thermal RCs which are in reasonable agreement with available experimental results.
Protein Thermodynamic Properties, Crystallisation, and the Hofmeister Series
Authors: Saridakis Emmanuel , Donta Katerina 
Published: May 2024

AbstractThe Hofmeister series is a series of ions ordered according to their ability to precipitate proteins. It has also been linked to a host of (bio)chemical phenomena. Several attempts over the years to correlate the series to the varying success of different salts in crystallising proteins have been largely inconclusive. A correlation, based on published data and crystallisation conditions for several proteins, is proposed here between some thermodynamic properties of proteins and the position in the Hofmeister series of the salts from which they preferentially crystallise. Namely, a high ratio between the entropic or enthalpic protein‐solvent interactions contribution to thermodynamic stability and the total thermodynamic stability of a given protein, indicate the protein's high propensity to crystallise in solutions of highly kosmotropic salts. Low such ratios on the other hand, indicate that chaotropic salts can be equally successful, i. e. that the protein in question is rather indifferent to the Hofmeister character of the salt. Testing various model proteins for crystallisation against screens containing salts found at different points on the Hofmeister series, as well as further bibliographic analysis, have yielded results that appear to largely corroborate this hypothesis. These conclusions may conceivably be used as a crystallisation predictive tool.
Charge Transport Approaches in Photocatalytic Supramolecular Systems Composing of Semiconductor and Molecular Metal Complex for CO2 Reduction
Authors: Ning Jiangqi , Chen Wei , Niu Qing , Li Liuyi , Yu Yan 
Published: May 2024

AbstractThe design of photocatalytic supramolecular systems composing of semiconductors and molecular metal complexes for CO2 reduction has attracted increasing attention. The supramolecular system combines the structural merits of semiconductors and metal complexes, where the semiconductor harvests light and undertakes the oxidative site, while the metal complex provides activity for CO2 reduction. The intermolecular charge transfer plays crucial role in ensuring photocatalytic performance. Here, we review the progress of photocatalytic supramolecular systems in reduction of CO2 and highlight the interfacial charge transfer pathways, as well as their state‐of‐the‐art characterization methods. The remaining challenges and prospects for further design of supramolecular photocatalysts are also presented.
Modular Cascade of Flow Reactors: Continuous Flow Synthesis of Water‐Insoluble Diazo Dyes in Aqueous System
Authors: Ouyang Jihong , Yang Wenbo , Guo Zhaoyan , Li Fujun , Liu Wendong , Guo Pengfei , Zhou Yumeng , Gao Dali , Zhang Lijing , Tao Shengyang 
Published: May 2024

AbstractContinuous flow synthesis is pivotal in dye production to address batch‐to‐batch variations. However, synthesizing water‐insoluble dyes in an aqueous system poses a challenge that can lead to clogging. This study successfully achieved the safe and efficient synthesis of azo dyes by selecting and optimizing flow reactor modules for different reaction types in the two‐step reaction and implementing cascade cooperation. Integrating continuous flow microreactor with continuous stirred tank reactor (CSTR) enabled the continuous flow synthesis of Sudan Yellow 3G without introducing water‐soluble functional groups or using organic solvents to enhance solubility. Optimizing conditions (acidity/alkalinity, temperature, residence time) within the initial modular continuous flow reactor resulted in a remarkable 99.5% isolated yield, 98.6 % purity, and a production rate of 2.90 g h−1. Scaling‐up based on different reactor module characteristics further increased the production rate to 74.4 g h−1 while maintaining high yield and purity. The construction of this small 3D‐printing modular cascaded reactor and process scaling‐up provide technical support for continuous flow synthesis of water‐insoluble dyes, particularly high‐market‐share azo dyes. Moreover, this versatile methodology proves applicable to continuous flow processes involving various homogeneous and heterogeneous reaction cascades.
A knock‐in allele of Hand2 expressing Dre recombinase
Authors: Plummer Nicholas W. , Smith Kathleen G. , Jensen Patricia 
Published: May 2024

SummaryHAND2 is a basic helix–loop–helix transcription factor with diverse functions during development. To facilitate the investigation of genetic and functional diversity among Hand2‐expressing cells in the mouse, we have generated Hand2Dre, a knock‐in allele expressing Dre recombinase. To avoid disrupting Hand2 function, the Dre cDNA is inserted at the 3′ end of the Hand2 coding sequence following a viral 2A peptide. Hand2Dre homozygotes can therefore be used in complex crosses to increase the proportion of useful genotypes among offspring. Dre expression in mid‐gestation Hand2Dre embryos is indistinguishable from wild‐type Hand2 expression, and HandDre efficiently recombines rox target sites in vivo. In combination with existing Cre and Flp mouse lines, Hand2Dre will therefore extend the ability to perform genetic intersectional labeling, fate mapping, and functional manipulation of subpopulations of cells characterized by developmental expression of Hand2.
The ups and downs of self‐criticism and disordered eating: Complementing Paranjothy and Wade's (2024) analysis by considering both trait and state perspectives
Authors: Kelly Allison C. , Katan Aleece , Zuroff David C. 
Published: May 2024

AbstractParanjothy and Wade's (2024) meta‐review reveals that individuals higher in the personality trait of self‐criticism consistently experience more disordered eating than those lower in the trait. The clinical implications of this meta‐review are important in that they suggest current theoretical models and clinical practices in the field of eating disorders should incorporate a greater focus on self‐criticism. Building on this exciting contribution, we highlight conceptual, practical, and empirical reasons why the field would benefit from supplementing this research on trait self‐criticism with investigations of state self‐criticism. We review research showing that self‐criticism levels vary not only between individuals, with some people chronically more self‐critical than others, but also within a person, with a given individual enacting relatively more self‐criticism during some moments and days than others. We then present emerging research showing that these periods of higher‐than‐usual self‐criticism are associated with more disordered eating. Thus, we emphasize the need to explore the factors that give rise to self‐critical states in daily life, and review preliminary findings on this topic. We highlight the ways in which research on within‐person variations in self‐criticism can complement research on trait self‐criticism to advance case formulation, prevention, and treatment in the field of eating disorders.
Identifying archived insect bulk samples using DNA metabarcoding: A case study using the long‐term Rothamsted Insect Survey
Authors: Petsopoulos Dimitrios , Cuff Jordan P. , Bell James R. , Kitson James J. N. , Collins Larissa , Boonham Neil , Morales‐Hojas Ramiro , Evans Darren M. 
Published: May 2024

AbstractInsect populations are declining in many parts of the world, but a lack of long‐term monitoring data is impeding our ability to understand and mitigate the causes of insect biodiversity loss. Whilst high‐throughput sequencing (HTS) approaches, such as DNA metabarcoding, have the potential to revolutionize insect biomonitoring through rapid scalable identification, it is unclear to what extent HTS can be applied to long‐term stored insect samples. Archived insect samples could inform forecasting and provide valuable information regarding past changes to biodiversity. Here, we assess the efficacy of DNA metabarcoding to identify archived samples from the longest passive monitoring scheme in the United Kingdom: the Rothamsted Insect Survey (RIS). With a focus on aphids as the target taxa of a national network of suction‐traps, we analyze a 16‐year time‐series of stored samples (2003–2018) using DNA metabarcoding from one of the RIS suction traps as an exemplar. We achieved this by using a non‐destructive DNA extraction protocol, ensuring the integrity of archival samples for further studies. We compared the identities of aphids determined by both metabarcoding (as inferred amplicon sequence variants [ASVs]) and morphological identification and found that metabarcoding detected most genera with varying success (mean > 76%). When comparing the two methods objectively (i.e., including taxa not detected morphologically), however, congruence decreased (51%). We show that minimum sequence copy thresholds can minimize metabarcoding false positives, but at the expense of introducing false negatives, highlighting the need for careful data curation. Detectability of taxa identified morphologically and similarity between the two methods did not significantly vary over time, demonstrating the viability of metabarcoding for screening archival samples. We discuss the advantages and challenges of metabarcoding for insect biomonitoring, particularly from archival samples, including improvements to sample handling, processing, and archiving. We highlight the wider potential of HTS approaches for stored samples from insect monitoring schemes, unlocking the immense potential of global historical time series.
Phosphorus‐based anodes for fast‐charging alkali metal ion batteries
Authors: Lan Xuexia , Li Zhen , Zeng Yi , Han Cuiping , Peng Jing , Cheng Hui‐Ming 
Published: May 2024

AbstractAdvancing fast‐charging technology is an important strategy for the development of alkali metal ion batteries (AMIBs). The exploitation of a new generation of anode material system with high‐rate performance, high capacity, and low risk of lithium/sodium/potassium plating is critical to realize fast‐charging capability of AMIBs while maintaining high energy density and safety. Among them, phosphorus‐based anodes including phosphorus anodes and metal phosphide anodes have attracted wide attention, due to their high theoretical capacities, safe reaction voltages, and natural abundance. In this review, we summarize the research progress of different phosphorus‐based anodes for fast‐charging AMIBs, including material properties, mechanisms for storing alkali metal ions, key challenges and solution strategies for achieving fast‐charging capability. Moreover, the future development directions of phosphorus‐based anodes in fast‐charging AMIBs are highlighted.image
Mechanistic analysis of acid gas storage and oil recovery in naturally fractured reservoirs using single matrix block approach
Authors: Shirzad Goran , Sadeghzadeh Zahra , Assareh Mehdi 
Published: May 2024

AbstractThe objective of this study is to assess the storage of acid gas, containing CO2 and H2S, in a depleted naturally fractured reservoir (NFR) using single matrix block (SMB) approach. The acid gas dissolution in oil is considered by Peng‐Robinson equation of state and compositional simulation. The PHREEQC package is used to determine acid gas solubility in formation brine. Three types of acid gases with different compositions are used for this study and their swelling behavior and miscibility in relation to the reservoir oil are analyzed. An SMB model, with a matrix block surrounded by fractures, is constructed, and validated for simulation of a real experiment. The simulation is conducted for synthetic and real reservoir fluids when the oil is in its residual saturation. A sensitivity analysis is performed to study the effects of key parameters, such as acid gas composition, reservoir pressure, permeability, porosity and matrix height on the storage capacity and oil recovery factor. The matrix has a volume of 27 m3 and about half of acid gas storage is achieved in the first 5 years while the simulations are run for 30 years. The results show that up to 90% of remained oil is recoverable, and more than 0.67 kmol of acid gas per cubic meter of matrix is stored whether matrix contains a real oil or a synthetic one. Higher storage is achieved for higher matrix porosities and heights and large H2S proportion in acid gas. In all cases about 10% of acid gas is trapped in water and the remaining 90% is dissolved in oil. The mineral trapping was more active in CO2‐rich acid gases. While about 10 kg of the matrix rock was dissolved in the acidic brine when the acid gas contained H2S, the amount of the dissolved minerals in acidic brine resulted from the injection of CO2‐rich acid gas was more than 16 kg. Finally, this study gives a comparative analysis of the storage performance of acid gas mixture and pure CO2. © 2024 Society of Chemical Industry and John Wiley & Sons, Ltd.