bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discovering patterns of online purchasing behaviour and a new‐product‐launch strategy
Authors: Hung Lun‐Ping 
Published: August 2011

AbstractSpurred by rapid development of computers and Internet technology, online shopping is gradually overtaking in‐store shopping, because of advantages such as convenience, more choice of products or services etc. Online stores must devote a great deal of time and resources to locating and attracting new customers. Growing a customer base requires first understanding customers and then providing the products or services they need, thus encouraging customers to purchase more. This paper develops a system to analyse customers’ purchasing behaviour and track shifts in their interests. Customers’ purchasing behaviour is measured using proposed standard product loyalty status and standard brand loyalty status. Using these metrics, together with the preference map established for each customer, a marketing specialist can easily locate potential customers to target when a company launches a new product. The new‐product‐launch strategy proposed in this paper can be used to create a list of potential customers for a product being launched under a variety of conditions. A prototype system has been built to test the feasibility of the proposed new‐product‐launch strategy. The result shows almost 40% of potential customers respond to the recommendation positively.
Managed Care and the Diffusion of Endoscopy in Fee‐for‐Service Medicare
Authors: Mobley Lee Rivers , Subramanian Sujha , Koschinsky Julia , Frech H. E. , Trantham Laurel Clayton , Anselin Luc 
Published: August 2011

ObjectiveTo determine whether Medicare managed care penetration impacted the diffusion of endoscopy services (sigmoidoscopy, colonoscopy) among the fee‐for‐service (FFS) Medicare population during 2001–2006.MethodsWe model utilization rates for colonoscopy or sigmoidoscopy as impacted by both market supply and demand factors. We use spatial regression to perform ecological analysis of county‐area utilization rates over two time intervals (2001–2003, 2004–2006) following Medicare benefits expansion in 2001 to cover colonoscopy for persons of average risk. We examine each technology in separate cross‐sectional regressions estimated over early and later periods to assess differential effects on diffusion over time. We discuss selection factors in managed care markets and how failure to control perfectly for market selection might impact our managed care spillover estimates.ResultsAreas with worse socioeconomic conditions have lower utilization rates, especially for colonoscopy. Holding constant statistically the socioeconomic factors, we find that managed care spillover effects onto FFS Medicare utilization rates are negative for colonoscopy and positive for sigmoidoscopy. The spatial lag estimates are conservative and interpreted as a lower bound on true effects. Our findings suggest that managed care presence fostered persistence of the older technology during a time when it was rapidly being replaced by the newer technology.
The Novel Leptospiral Surface Adhesin Lsa20 Binds Laminin and Human Plasminogen and Is Probably Expressed during Infection
Authors: Mendes Renata Siqueira , Von Atzingen Marina , de Morais Zenaide Maria , Gonçales Amane Paldes , Serrano Solange M. T. , Asega Amanda F. , Romero Eliete Caló , Vasconcellos Silvio Arruda , Nascimento Ana Lucia Tabet O. 
Published: August 2011

ABSTRACT

Leptospirosis is an emerging infectious disease caused by pathogenic species of
Leptospira
. In this work, we report the cloning, expression, purification, and characterization of two predicted leptospiral outer membrane proteins, LIC11469 and LIC11030. The LIC11469 protein is well conserved among leptospiral strains, while LIC11030 was identified only in
L
eptospira
interrogans
. We confirmed by surface proteolysis of intact leptospires with proteinase K that these proteins are most likely new surface leptospiral proteins. The recombinant proteins were evaluated for their capacity to attach to extracellular matrix (ECM) components and to plasminogen. The leptospiral protein encoded by LIC11469, named Lsa20 (leptospiral surface adhesin of 20 kDa), binds to laminin and to plasminogen. The binding with both components was not detected when Lsa20 was previously denatured or blocked with anti-Lsa20 antibodies. Moreover, Lsa20 binding to laminin was also confirmed by surface plasmon resonance (SPR). Laminin competes with plasminogen for binding to Lsa20, suggesting the same ligand-binding site. Lsa20-bound plasminogen could be converted to enzymatically active plasmin, capable of cleaving plasmin substrate
d
-valyl-leucyl-lysine
-p-
nitroanilide dihydrochloride. Lsa20 was recognized by antibodies in confirmed-leptospirosis serum samples, suggesting that this protein is expressed during infection. Taken together, our results indicate that Lsa20 is a novel leptospiral adhesin that in concert with the host-derived plasmin may help the bacteria to adhere and to spread through the hosts.
Leukocyte integrin activation mediates transient neutropenia after G-CSF administration
Authors: Donahue Robert E. , Tuschong Laura , Bauer Thomas R. , Yau Yu Ying , Leitman Susan F. , Hickstein Dennis D. 
Published: August 2011

Abstract
After administration of granulocyte colony-stimulating factor (G-CSF), there is a marked, albeit transient, drop in circulating neutrophils. To determine the role of leukocyte integrins in this disappearance, a dog having canine leukocyte adhesion deficiency (CLAD) or CLAD dogs who had undergone gene correction either by matched littermate allogeneic transplant or autologous gene therapy were evaluated. Shortly after G-CSF administration, a dramatic, yet transient, neutropenia was observed in the control littermates. This neutropenia was not as marked in the CLAD dogs. In all instances, it was CD18+ neutrophils that preferentially egressed from the circulation. The association of CD18 with this rapid loss suggested leukocyte integrin activation after G-CSF administration. To determine the activation status of the integrin, a monoclonal antibody recognizing the activated α-subunit cation binding domain (mAb24) was used to evaluate human leukocytes after G-CSF administration. Mirroring the dramatic decrease in circulating neutrophil numbers, there was a dramatic and specific increase in the activation of the α-subunit after G-CSF expression on polymorphonuclear leukocytes. This activation, like the drop in neutrophil count, was transient. These results demonstrate that the leukocyte integrin on circulating neutrophils is transiently activated after G-CSF administration and mediates the transient neutropenia observed after G-CSF administration.
Cug2 is essential for normal mitotic control and CNS development in zebrafish
Authors: Kim Hyun-Taek , So Ju-Hoon , Jung Seung-Hyun , Ahn Dae-Gwon , Koh Wansoo , Kim Nam-Soon , Kim Soo-Hyun , Lee Soojin , Kim Cheol-Hee 
Published: August 2011

Abstract
Background
We recently identified a novel oncogene, Cancer-upregulated gene 2 (CUG2), which is essential for kinetochore formation and promotes tumorigenesis in mammalian cells. However, the in vivo function of CUG2 has not been studied in animal models.

Results
To study the function of CUG2 in vivo, we isolated a zebrafish homologue that is expressed specifically in the proliferating cells of the central nervous system (CNS). Morpholino-mediated knockdown of cug2 resulted in apoptosis throughout the CNS and the development of neurodegenerative phenotypes. In addition, cug2-deficient embryos contained mitotically arrested cells displaying abnormal spindle formation and chromosome misalignment in the neural plate.

Conclusions
Therefore, our findings suggest that Cug2 is required for normal mitosis during early neurogenesis and has functions in neuronal cell maintenance, thus demonstrating that the cug2 deficient embryos may provide a model system for human neurodegenerative disorders.
Preventive effect of Ibrolipim on suppressing lipid accumulation and increasing lipoprotein lipase in the kidneys of diet-induced diabetic minipigs
Authors: Liu Yi , Wang Zong Bao , Yin Wei Dong , Li Qin Kai , Cai Man Bo , Yu Jian , Li Hong Guang , Zhang Chi , Zu Xiu Hong 
Published: August 2011

AbstractBackgroundThe role of renal lipoprotein lipase (LPL)per sein kidney diseases is still controversial and obscure. The purpose of this study was to observe the preventive effects of Ibrolipim, a LPL activator, on lipid accumulation and LPL expression in the kidneys of minipigs fed a high-sucrose and high-fat diet (HSFD).MethodsMale Chinese Bama minipigs were fed a control diet or HSFD with or without 0.1 g/kg/day Ibrolipim for 5 months. Body weight, plasma glucose, insulin, lipids, LPL activity, and urinary microalbumin were measured. Renal tissue was obtained for detecting LPL activity and contents of triglyceride and cholesterol, observing the renal lipid accumulation by Oil Red O staining, and examining the mRNA and protein expression of LPL by real time PCR, Western Blot and immunohistochemistry.ResultsFeeding HSFD to minipigs caused weight gain, hyperglycemia, hyperinsulinemia, hyperlipidemia and microalbuminuria. HSFD increased plasma LPL activity while it decreased the mRNA and protein expression and activity of LPL in the kidney. The increases in renal triglyceride and cholesterol contents were associated with the decrease in renal LPL activity of HSFD-fed minipigs. In contrast, supplementing Ibrolipim into HSFD lowered body weight, plasma glucose, insulin, triglyceride and urinary albumin concentrations while it increased plasma total cholesterol and HDL-C. Ibrolipim suppressed the renal accumulation of triglyceride and cholesterol, and stimulated the diet-induced down-regulation of LPL expression and activity in the kidney.ConclusionsIbrolipim exerts renoprotective and hypolipidemic effectsviathe increase in renal LPL activity and expression, and thus the increased expression and activity of renal LPL play a vital role in suppressing renal lipid accumulation and ameliorating proteinuria in diet-induced diabetic minipigs.
Fatal invasive cervical cancer secondary to untreated cervical dysplasia: a case report
Authors: Braun Stephan , Reimer Daniel , Strobl Isolde , Wieland Ulrike , Wiesbauer Petra , Müller-Holzner Elisabeth , Fessler Siegfried , Scherer Arthur , Marth Christian , Zeimet Alain G 
Published: August 2011

Abstract

Introduction
Well-documented cases of untreated cervical intra-epithelial dysplasia resulting in fatal progression of invasive cervical cancer are scarce because of a long pre-invasive state, the availability of cervical cytology screening programs, and the efficacy of the treatment of both pre-invasive and early-stage invasive lesions.

Case presentation
We present a well-documented case of a 29-year-old Caucasian woman who was found, through routine conventional cervical cytology screening, to have pathologic Papanicolaou (Pap) grade III D lesions (squamous cell abnormalities). She subsequently died as a result of human papillomavirus type 18-associated cervical cancer after she refused all recommended curative therapeutic procedures over a period of 13 years.

Conclusion
This case clearly demonstrates a caveat against the promotion and use of complementary alternative medicine as pseudo-immunologic approaches outside evidence-based medicine paths. It also demonstrates the impact of the individualized demands in diagnosis, treatment and palliative care of patients with advanced cancer express their will to refuse evidence-based treatment recommendations.
Cardiogenic shock following administration of propofol and fentanyl in a healthy woman: a case report
Authors: Renilla González Alfredo , Lozano Martinez-Luengas Iñigo , Benito Martín Eva María , Secades González Sandra , Álvarez Pichel Irene , Álvarez Martinez Paloma , Santamarta Liébana Elena , Díaz Molina Beatriz 
Published: August 2011

Abstract

Introduction
Cardiogenic shock is very uncommon in healthy people. The differential diagnosis for patients with acute heart failure in previously healthy hearts includes acute myocardial infarction and myocarditis. However, many drugs can also depress myocardial function. Propofol and fentanyl are frequently used during different medical procedures. The cardiovascular depressive effect of both drugs has been well established, but the development of cardiogenic shock is very rare when these agents are used.

Case presentation
After a minor surgical intervention, a 32-year-old Caucasian woman with no significant medical history went into sudden hemodynamic deterioration due to acute heart failure. An urgent echocardiogram showed severe biventricular dysfunction and an estimated left ventricular ejection fraction of 20%. Extracorporeal life support and mechanical ventilation were required. Five days later her ventricular function had fully recovered, which allowed the progressive withdrawal of medical treatment. Prior to her hospital discharge, cardiac MRI showed neither edema nor pathological deposits on the delayed contrast enhancement sequences. At her six-month follow-up examination, the patient was asymptomatic and did not require treatment.

Conclusion
Although there are many causes of cardiogenic shock, the presence of abrupt hemodynamic deterioration and the absence of a clear cause could be related to the use of propofol and fentanyl.
Incidence of Tuberculosis Among Korean Patients with Ankylosing Spondylitis Who Are Taking Tumor Necrosis Factor Blockers
Authors: KIM EUN-MI , UHM WAN-SIK , BAE SANG-CHEOL , YOO DAE-HYUN , KIM TAE-HWAN 
Published: August 2011

Objective.To assess the incidence and relative risk of new tuberculosis (TB) infections in Korean patients with ankylosing spondylitis (AS) and patients with AS who are undergoing treatment with tumor necrosis factor (TNF) blockers.Methods.New cases of TB were identified by reviewing the medical records of 919 patients with AS not treated with TNF blockers and those of 354 patients with AS treated with adalimumab (n = 66), infliximab (n = 78), or etanercept (n = 210) between 2002 and 2009. Reference data were obtained from the Korean National Tuberculosis Association.Results.The mean incidence rate of TB was 69.8 per 100,000 person-years (PY) in the general population, 308 per 100,000 PY in the TNF blocker-naive AS cohort, and 561 per 100,000 PY in the TNF blocker-exposed AS cohort. The incidence rate of TB in the infliximab-treated AS cohort (540 per 100,000 PY) was higher than that in the adalimumab-treated AS cohort (490 per 100,000 PY). No cases of TB occurred in the etanercept-treated AS cohort. Comparing the relative risks of TB infections between the TNF blocker-exposed AS cohort and the TNF blocker-naive AS cohort, no statistically significant difference was identified (risk ratio 0.53; 95% CI 0.144–1.913).Conclusion.The risk of TB was higher in the TNF blocker-naive AS cohort than it was in the general population. However, the risk of TB was not increased in the TNF blocker-exposed AS cohort compared with the TNF blocker-naive AS cohort. Among patients with AS, etanercept is associated with a lower risk of TB compared with monoclonal antibodies.
A de novo 2.1‐Mb deletion of 13q12.11 in a child with developmental delay and minor dysmorphic features
Authors: Der Kaloustian Vazken M. , Russell Laura , Aradhya Swaroop , Richard Gabriele , Rosenblatt Bernard , Melançon Serge 
Published: August 2011

AbstractWe report on a patient with an interstitial deletion at 13q12.11. He had mild developmental delay, craniofacial dysmorphism, a pectus excavatum, narrow shoulders, malformed toes, and café‐au‐lait spots. Array CGH analysis disclosed a de novo deletion spanning 2.1 Mb, within cytogenetic band 13q12.11. The deletion produces hemizygozity for 16 known genes, among which GJA3, GJB2, GJB6, IFT88, LATS2, and FGF9 have potential clinical significance. The observed phenotype may be due to mutation in one of the 16 genes, or to a combination of deletion and/or mutation in a number of them. © 2011 Wiley‐Liss, Inc.
The functions of crystallizable ethylene‐propylene copolymers in the formation of multiple phase morphology of high impact polypropylene
Authors: Tong Cuiyan , Lan Yang , Chen Ye , Chen Yong , Yang Decai , Yang Xiaoniu 
Published: August 2011

AbstractThe functions of crystallizable ethylene‐propylene copolymers in the formation of multiple phase morphology of high impact polypropylene (hiPP) were studied by solvent extraction fractionation, transmission electron microscopy (TEM), selected area electron diffraction (SAED), nuclear magnetic resonance (13C‐NMR), and selected reblending of different fractions of hiPP. The results indicate that hiPP contains, in addition to polypropylene (PP) and amorphous ethylene‐propylene random copolymer (EPR) as well as a small amount of polyethylene (PE), a series of crystallizable ethylene‐propylene copolymers. The crystallizable ethylene‐propylene copolymers can be further divided into ethylene‐propylene segmented copolymer (PE‐s‐PP) with a short sequence length of PE and PP segments, and ethylene‐propylene block copolymer (PE‐b‐PP) with a long sequence length of PE and PP blocks. PE‐s‐PP and PE‐b‐PP participate differently in the formation of multilayered core‐shell structure of the dispersed phase in hiPP. PE‐s‐PP (like PE) constructs inner core, PE‐b‐PP forms outer shell, while intermediate layer is resulted from EPR. The main reason of the different functions of the crystallizable ethylene‐propylene copolymers is due to their different compatibility with the PP matrix. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
An educational toolbox for performance analysis of line‐start permanent magnet synchronous motors
Authors: Isfahani Arash Hassanpour , Vaez‐Zadeh Sadegh , Hasanzadeh Saeed 
Published: August 2011

AbstractThis article presents the development of an education purpose toolbox for performance analysis of three‐phase line start permanent magnet synchronous motors. The motor state‐space equations are implemented in Simulink and an easy to use graphic user interface is designed. The starting performance of a typical motor is then simulated in different conditions as a case study. This toolbox provides a friendly and efficient environment for students to become familiar with the performance of this type of motor which is receiving increasing attention in the industry and academic. An experimental study is also carried out to validate the toolbox. © 2011 Wiley Periodicals, Inc. Comput Appl Eng Educ 22:452–462, 2014; View this article online at wileyonlinelibrary.com/journal/cae; DOI 10.1002/cae.10.1002/cae.20569
On the Egress of Carbon Monoxide from Myoglobin
Authors: Pietra Francesco 
Published: August 2011

AbstractThe pathways of escape of carbon monoxide (CO) from sperm whale myoglobin were investigated by means of a biased form of all‐atoms molecular dynamics (RAMD), whereby a weak, randomly oriented force is applied to the center of mass of CO. The force only persists if the direction taken by CO continues for a given period of time, otherwise a new direction is randomly chosen. A statistically significant number of RAMD runs gave distinct responses according to the level of approximations used for the model. Thus, with rigid bonds to all H‐atoms, several portals for CO egress toward the solvent, besides the main H64 gate, were identified, like in recently published unbiased massive MD, six orders of magnitude acceleration of CO escape in RAMD notwithstanding. In contrast, by removing the approximation of rigid bonds in the model, only one of these extra portals was identified, besides the H64 portal. Sticking to this all‐free‐bonds model, Perutz's early suggestion that the H64 imidazole must rotate ‘out’ toward the solvent in order that CO can cross the H64 gate was directly implemented. RAMD Simulations with this model led to CO egress from the H64 gate only, reconciling theory with experiments.
Clinical profiles and long‐term outcomes of patients with pancreatic ductal adenocarcinoma and diabetes mellitus
Authors: Ben Qiwen , Xu Maojin , Jiang Ying , Yuan Yaozong , Wang Kaixuan , Fang Jun , Li Zhaoshen 
Published: August 2011

AbstractBackgroundDiabetes mellitus (DM) is considered to be a possible risk factor and/or a manifestation of pancreatic ductal adenocarcinoma (PDAC). This study is aimed at analysing the potential association of diabetes mellitus with the development and pathogenic degrees of PDAC and post‐surgical survival of Chinese Han PDAC patients.MethodsA total of 1123 patients with PDAC were recruited and included 256 patients with diabetes mellitus within 2 years (new‐onset) and 62 patients with diabetes mellitus ≥ 2 years (long‐standing). Additional 466 patients with type 2 diabetes mellitus were included in this study. Their clinical characteristics and long‐term outcomes were analysed.ResultsIn comparison with patients with type 2 diabetes mellitus alone, PDAC patients with new‐onset diabetes mellitus had an older onset age of diabetes mellitus and lower body mass index (BMI). Among PDAC cases, patients with new‐onset diabetes mellitus were associated with neural invasion, poor tumour differentiation and shorter post‐surgical survival. However, more than half of these patients became euglycemic after surgical resection of tumours.ConclusionsPDAC patients developed new‐onset diabetes mellitus at an older age, and they had shorter post‐surgical survival. The underlying mechanisms by which comorbid diabetes mellitus affect the clinical profiles and outcomes of PDAC patients deserve further researches. Copyright © 2011 John Wiley & Sons, Ltd.
Efficient Synthesis of (3E)‐3‐[Amino(aryl)methylidene]chromane‐2,4‐diones (=(3E)‐3‐[Amino(aryl)methylene]‐2H‐1‐benzopyran‐2,4(3H)‐diones) via a Three‐Component Reaction
Authors: Ghabraie Elmira , Bararjanian Morteza , Balalaie Saeed , Rominger Frank , Bijanzadeh Hamid Reza 
Published: August 2011

AbstractThe Michael‐type addition of a 4‐hydroxycoumarin (=4‐hydroxy‐2H‐1‐benzopyran‐2‐one) 1 to a β‐nitrostyrene (=(2‐nitroethenyl)benzene) 2 in the presence of AcONH4 leads to substituted (3E)‐3‐[amino(aryl)methylidene]chroman‐2,4‐diones (=(3E)‐3‐[amino(aryl)methylene]‐2H‐1‐benzopyran‐2,4(3H)‐diones) 4 (Table 1). High yields, short reaction time, and easy workup are advantages of this novel one‐pot three‐component reaction.
Synthesis of β‐Hydroxy α‐Sulfanyl Esters by Using Nanocrystalline Magnesium Oxide
Authors: Mahendar Koosam , Sarma A. V. S. , Raghavaiah P. , Kantam Mannepalli Lakshmi , Klabunde Kenneth J. 
Published: August 2011

AbstractAldol‐type reaction between electron deficient aldehydes and sulfonium salts to afford the corresponding β‐hydroxy α‐sulfanyl esters in moderate‐to‐good yields by using nanocrystalline MgO is described. The sulfanyl group is a useful group for further transformations in organic synthesis. Low Rf‐value isomer is anti‐configured as revealed by X‐ray diffraction study and consistent with the assignment of 1H‐NMR spectrum.
Metal‐Free Multicomponent Synthesis of (α‐Aminoalkyl)phosphonates Using 2,4,6‐Trichloro‐1,3,5‐triazine
Authors: Das Biswanath , Kumar Avula Satya , Kumar Jayprakash Narayan 
Published: August 2011

Abstract(α‐Aminoalkyl)phosphonates have efficiently been synthesized by multicomponent reaction of aldehydes, amines, and triethyl phosphite in the presence of 2,4,6‐trichloro‐1,3,5‐triazine at room temperature. The products are formed in high yields (83–91%) within 0.5–1 h.