bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of tryptanthrin as an antibacterial reagent inhibiting Vibrio splendidus
Authors: Yang Huirong , Li Ya , Shi Weibo , Zhang Weiwei 
Published: May 2024

Abstract
Isolates of Vibrio splendidus are ubiquitously presented in various marine environments, and they can infect diverse marine culture animals, leading to high mortality and economic loss. Therefore, a control strategy of the infection caused by V. splendidus is urgently recommended. Tryptanthrin is a naturally extracted bioactive chemical with antimicrobial activity to other bacteria. In this study, the effects of tryptanthrin on the bacterial growth and virulence-related factors of one pathogenic strain V. splendidus AJ01 were determined. Tryptanthrin (10 μg/mL) could completely inhibit the growth of V. splendidus AJ01. The virulence-related factors of V. splendidus AJ01 were affected in the presence of tryptanthrin. Tryptanthrin resulted an increase in biofilm formation, but lead to reduction in the motility and hemolytic activity of V. splendidus cells. In the cells treated with tryptanthrin, two distinctly differentially expressed extracellular proteins, proteases and flagellum, were identified using SDS–PAGE combined with LC–MS. Real-time reverse transcriptase PCR confirmed that the genes involved in the flagellar formation and hemolysin decreased, whereas specific extracellular proteases and the genes involved in the biofilm formation were upregulated. Two previously annotated luxOVs genes were cloned, and their expression levels were analyzed at different cell densities. Molecular docking was performed to predict the interaction between LuxOVs and ATP/tryptanthrin. The two sigma-54-dependent transcriptional regulators showed similar ATP or tryptanthrin binding capacity but with different sites, and the direct competitive binding between ATP and tryptanthrin was present only in their binding to LuxO1. These results indicated that tryptanthrin can be used as a bactericide of V. splendidus by inhibiting the growth, bacterial flagella, and extracellular proteases, but increasing the biofilm. Sigma-54-dependent transcriptional regulator, especially the quorum sensing regulatory protein LuxO1, was determined to be the potential target of tryptanthrin.

Key points
• Tryptanthrin inhibited the growth of V. splendidus in a dose-dependent manner.
• The effect of tryptanthrin on the virulence factors of V. splendidus was characterized.
• LuxO was the potential target for tryptanthrin based on molecular docking.
PET imaging of Aspergillus infection using Zirconium-89 labeled anti-β-glucan antibody fragments
Authors: Lai Jianhao , Shah Swati , Martinez-Orengo Neysha , Knight Rekeya , Alemu Eyob , Turner Mitchell L. , Wang Benjamin , Lyndaker Anna , Shi Jianfeng , Basuli Falguni , Hammoud Dima A. 
Published: May 2024

Abstract
Purpose
Invasive fungal diseases, such as pulmonary aspergillosis, are common life-threatening infections in immunocompromised patients and effective treatment is often hampered by delays in timely and specific diagnosis. Fungal-specific molecular imaging ligands can provide non-invasive readouts of deep-seated fungal pathologies. In this study, the utility of antibodies and antibody fragments (Fab) targeting β-glucans in the fungal cell wall to detect Aspergillus infections was evaluated both in vitro and in preclinical mouse models.

Methods
The binding characteristics of two commercially available β-glucan antibody clones and their respective antigen-binding Fabs were tested using biolayer interferometry (BLI) assays and immunofluorescence staining. In vivo binding of the Zirconium-89 labeled antibodies/Fabs to fungal pathogens was then evaluated using PET/CT imaging in mouse models of fungal infection, bacterial infection and sterile inflammation.

Results
One of the evaluated antibodies (HA-βG-Ab) and its Fab (HA-βG-Fab) bound to β-glucans with high affinity (KD = 0.056 & 21.5 nM respectively). Binding to the fungal cell wall was validated by immunofluorescence staining and in vitro binding assays. ImmunoPET imaging with intact antibodies however showed slow clearance and high background signal as well as nonspecific accumulation in sites of infection/inflammation. Conversely, specific binding of [89Zr]Zr-DFO-HA-βG-Fab to sites of fungal infection was observed when compared to the isotype control Fab and was significantly higher in fungal infection than in bacterial infection or sterile inflammation.

Conclusions
[89Zr]Zr-DFO-HA-βG-Fab can be used to detect fungal infections in vivo. Targeting distinct components of the fungal cell wall is a viable approach to developing fungal-specific PET tracers.
Superior semicircular canal dehiscence postoperative outcomes: a case series of 350 repairs
Authors: Mekonnen Mahlet , Lum Meachelle , Duong Courtney , Rana Shivam , Mozaffari Khashayar , Hovis Gabrielle E. A. , Yang Isaac 
Published: May 2024

Abstract

Background
Superior Semicircular Canal Dehiscence (SSCD) is a dehiscence of the otic capsule which normally lies over the superior semicircular canal. This database constitutes the largest series of SSCD patients to date.

Objective
To determine what preoperative factors, if any, contribute to postoperative outcomes and evaluate symptom resolution in a large SSCD patient cohort.

Methods
A single-institution, retrospective chart review collected patient demographics, intraoperative findings, and pre-and postoperative symptoms. Fisher's exact t-test was performed for unpaired categorical variables, with a significance level of p < 0.05.

Results
350 SSCD repairs were performed. The median age was 52 years (range: 17—86 years, ± 6.4 years), and the median follow-up duration was 4.6 months (range: 0.03—59.5 months, ± 6.8 months). Preoperative hearing loss was significantly associated with female sex (p = 0.0028). The most reported preoperative symptoms were tinnitus (77.4%), dizziness (74.0%), autophony (66.3%), amplification (63.7%), and disequilibrium (62.6%). Between patients who received unilateral versus bilateral SSCD repair, the greatest postoperative symptomatic resolution was seen in autophony (74.9%, p < 0.001), amplification (77.3%, p = 0.00027), hyperacusis (77.4%, p = 0.023), hearing (62.9%, p = 0.0063), and dizziness (54.6%, p < 0.001) for patients with unilateral SSCD repair.

Conclusion
Surgical repair via the middle cranial fossa approach can significantly resolve auditory, vestibular, and neurological symptoms of patients with SSCD. Although this is one of the largest single-institution SSCD studies to date, future multi-institutional, prospective studies would be beneficial to validate these results.
Intra-operative extracorporeal irradiation of tumour-invaded craniotomy bone flap in meningioma: a case series
Authors: Cook William H. , Burton Katherine , Jefferies Sarah J. , Duke Simon L. , Jena Rajesh , Burnet Neil G. , Kirollos Ramez W. , Helmy Adel E. , Santarius Thomas 
Published: May 2024

Abstract

Background
Extracorporeal irradiation of tumorous calvaria (EITC) can be performed to restore function and form of the skull after resection of bone-invasive meningioma. We sought to examine the rate of tumour recurrence and other selected outcomes in patients undergoing meningioma resection and EITC.

Methods
Retrospective single-centre study of adult patients undergoing meningioma resection and EITC between January 2015 and November 2022 at a tertiary neurosurgical centre. Patient demographics, surgery data, tumour data, use of adjuvant therapy, surgical complications, and tumour recurrences were collected.

Results
Eighteen patients with 11 (61%) CNS WHO grade 1, 6 (33%) grade 2, and 1 (6%) grade 3 meningiomas were included. Median follow-up was 42 months (range 3–88). Five (28%) patients had a recurrence, but none were associated with the bone flap. Two (11%) wound infections requiring explant surgery occurred. Six (33%) patients required a further operation. Two operations were for recurrences, one was for infection, one was a washout and wound exploration but no evidence of infection was found, one patient requested the removal of a small titanium implant, and one patient required a ventriculoperitoneal shunt for a persistent CSF collection. There were no cases of bone flap resorption and cosmetic outcome was not routinely recorded.

Conclusion
EITC is feasible and fast to perform with good outcomes and cost-effectiveness compared to other reconstructive methods. We observed similar recurrence rates and lower infection rates requiring explant compared to the largest series of cranioplasty in meningioma. Cosmetic outcome is universally under-reported and should be reported in future studies.
Perception of optical illusions in ungulates: insights from goats, sheep, guanacos and llamas
Authors: Berardo Caterina , Holland Ruben , Schaffer Alina , Lopez Caicoya Alvaro , Liebal Katja , Valsecchi Paola , Amici Federica 
Published: May 2024

Abstract
Optical illusions have long been used in behavioural studies to investigate the perceptual mechanisms underlying vision in animals. So far, three studies have focused on ungulates, providing evidence that they may be susceptible to some optical illusions, in a way similar to humans. Here, we used two food-choice tasks to study susceptibility to the Müller-Lyer and Delboeuf illusions in 17 captive individuals belonging to four ungulate species (Lama guanicoe, Lama glama
,
Ovis aries, Capra hircus). At the group level, there was a significant preference for the longer/larger food over the shorter/smaller one in control trials. Additionally, the whole group significantly preferred the food stick between two inward arrowheads over an identical one between two outward arrowheads in experimental trials of the Müller-Lyer task, and also preferred the food on the smaller circle over an identical one on the larger circle in the experimental trials of the Delboeuf task. Group-level analyses further showed no significant differences across species, although at the individual level we found significant variation in performance. Our findings suggest that, in line with our predictions, ungulates are overall susceptible to the Müller-Lyer and the Delboeuf illusions, and indicate that the perceptual mechanisms underlying size estimation in artiodactyls might be similar to those of other species, including humans.
Hitting the Rewind Button: Imagining Analogue Trauma Memories in Reverse Reduces Distressing Intrusions
Authors: Rattel Julina A. , Danböck Sarah , Miedl Stephan F. , Liedlgruber Michael , Wilhelm Frank H. 
Published: May 2024

Abstract
Background
Intrusive re-experiencing of trauma is a core symptom of posttraumatic stress disorder. Intrusive re-experiencing could potentially be reduced by ‘rewinding’, a new treatment approach assumed to take advantage of reconsolidation-updating by mentally replaying trauma fast-backward.

Methods
The present analogue study was the first to investigate ‘rewinding’ in a controlled laboratory setting. First, 115 healthy women watched a highly aversive film and were instructed to report film-related intrusions during the following week. Twenty-four hours after film-viewing, participants reporting at least one intrusion (N = 81) were randomly allocated to an intervention (fast-backward, or fast-forward as active control condition) or a passive control condition. Intervention groups reactivated their trauma memory, followed by mentally replaying the aversive film either fast-backward or fast-forward repeatedly.

Results
Results indicate that replaying trauma fast-backward reduced intrusion load (intrusion frequency weighted for intrusion distress) compared to the passive group, whereas replaying fast-forward did not. No above-threshold differences between fast-backward and fast-forward emerged.

Conclusion
Present findings strengthen the view that ‘rewinding’ could be a promising intervention to reduce intrusions.
Gendered Parenthood-Employment Gaps from Midlife: A Demographic Perspective Across Three Different Welfare Systems
Authors: Lorenti Angelo , Nisén Jessica , Mencarini Letizia , Myrskylä Mikko 
Published: May 2024

Abstract
Women’s labor force participation has increased in Western countries, but gender gaps remain, especially among parents. Using a novel comparative perspective, we assess women’s and men’s employment trajectories from midlife onward by parity and education. We provide insights into the gendered parenthood-employment gaps examining the long-term implications of parenthood beyond the core childbearing ages by decomposing years lived between ages 40–74, in years of employment, joblessness, and retirement. Using multistate incidence-based life tables, we compare different cultural and institutional contexts: Finland, Italy, and the USA. Our results document large cross-national variation, with education playing a key role. In Finland, the number of years of employment increases with parity for women and men, and the gender gap is small; in the USA, the relationship between parity and years of employment is relatively flat, although a gender gap emerges among those with two or more children; in Italy, the number of years of employment decreases sharply for women as parity increases, while it increases for men. Notably, education has a similar positive impact on years of employment across all groups in Finland. In contrast, in the USA and Italy, the gender gap is only half as large among highly educated mothers as it is among low educated mothers. The employment trajectories of childless women and men differ greatly across countries.
Towards autonomous learning and optimisation in textile production: data-driven simulation approach for optimiser validation
Authors: Kins Ruben , Möbitz Christian , Gries Thomas 
Published: May 2024

AbstractThe textile industry is a traditional industry branch that remains highly relevant in Europe. The industry is under pressure to remain profitable in this high-wage region. As one promising approach, data-driven methods can be used for process optimisation in order to reduce waste, increase profitability and relieve mental burden on staff members. However, approaches from research rarely get adopted into practice. We identify the high dimensionality of textile production processes leading to high model uncertainty as well as an incomplete problem formulation as the two main problems. We argue that some form of an autonomous learning agent can address this challenge, when it safely explores advantageous, unknown new settings by interacting with the process. Our main goal is to facilitate the adoption of promising research into practical applications. The main contributions of this paper include the derivation and formulation of a probabilistic optimisation problem for high-dimensional, stationary production processes. We also create a highly adaptable simulation of the textile carded nonwovens production process in Python that implements the optimisation problem. Economic and technical behavior of the process is approximated using both Gaussian Process Regression (GPR) models trained with industrial data as well as physics-motivated explicit models. This ’simulation first’-approach makes the development of autonomous learning agents for practical applications feasible because it allows for cheap testing and validation before physical trials. Future work will include the comparison of the performance of different agent approaches.
Explosion Rates for Continuous-State Branching Processes in a Lévy Environment
Authors: Cardona-Tobón Natalia , Pardo Juan Carlos 
Published: May 2024

AbstractHere, we study the long-term behaviour of the non-explosion probability for continuous-state branching processes in a Lévy environment when the branching mechanism is given by the negative of the Laplace exponent of a subordinator. In order to do so, we study the law of this family of processes in the infinite mean case and provide necessary and sufficient conditions for the process to be conservative, i.e. that the process does not explode in finite time a.s. In addition, we establish precise rates for the non-explosion probabilities in the subcritical and critical regimes, first found by Palau et al. (ALEA Lat Am J Probab Math Stat 13(2):1235–1258, 2016) in the case when the branching mechanism is given by the negative of the Laplace exponent of a stable subordinator.
Longitudinal Change in Appearance-Related Social Media Consciousness and Depressive Symptoms: A Within-Person Analysis during Early-to-Middle Adolescence
Authors: Maheux Anne J. , Laurenceau Jean-Philippe , Roberts Savannah R. , Nesi Jacqueline , Widman Laura , Choukas-Bradley Sophia 
Published: May 2024

AbstractOnline appearance preoccupation may put adolescents at risk of developing mental health challenges, perhaps especially during early-to-middle adolescence. A random intercept cross-lagged panel model assessed within-person associations between appearance-related social media consciousness and depressive symptoms over three time-points with three months between waves. The sample (n = 1594) included U.S. adolescents aged 11–15 (Mage = 13; 47% girls, 46% boys, 7% another gender; 37% Latine, 33% White, 18% Black, 7% Asian). Within-person increases in appearance-related social media consciousness were associated with subsequent increases in depressive symptoms, but not vice versa. There was no evidence of gender differences and results were robust to controlling for both time on social media and offline self-objectification. Thus, online appearance concerns precede mental health challenges during early and middle adolescence.
Clarifying the Links between Perceived Stress and Depressiveness: a Longitudinal Study of COVID-19’s Effects on Adolescents in Germany
Authors: Gniewosz Gabriela 
Published: May 2024

AbstractYoung people are navigating an increasingly uncertain and unstable social and economic environment, further complicated by COVID-19. Individual resources and vulnerabilities, such as mental health and sensitivity to stress, play a significant role in how well youth adapt to the career paths and living conditions altered by the pandemic, a dynamic that is not yet well understood. This study examined the role of COVID-19 on the intertwined relation between perceived stress and depressiveness (negative and positive affect) in adolescents, focusing on gender differences. Longitudinal data from 673 German adolescents (Mage = 16.8 years, SDage = 0.91; female = 59%) were collected in three waves, before (T1) and during the pandemic (T2, T3). Using Latent Change Score models, the bidirectional relation between perceived stress and depressiveness was analyzed, considering gender as a moderator. The results showed that adolescents who found their situation stressful were at risk of developing depressiveness at the outbreak of the pandemic and throughout its progression. As the pandemic progressed, an increase in positive affect was linked to heightened perceived stress. Gender-specific differences were particularly evident in the levels of perceived stress and depressiveness, with women being more vulnerable. This study highlights how vulnerabilities in stress perception affect adolescents’ mental health, with gender-specific differences underscoring the need for tailored mental health measures.
Promoting Adolescents’ Heritage Cultural Identity Development: Exploring the Role of Autonomy and Relatedness Satisfaction in School-Based Interventions
Authors: Hölscher Sophie I. E. , Schachner Maja K. , Juang Linda P. , Altoè Gianmarco 
Published: May 2024

AbstractGiven the significance of heritage cultural identity for optimal adolescent development, it is imperative to investigate factors influencing the efficacy of interventions aimed at promoting heritage cultural identity. Using latent profile cluster analysis and multinomial logistic regressions, this longitudinal study examined how autonomy and relatedness need satisfaction at school (1) related to heritage cultural identity development trajectories, and (2) moderated effects of a school-based intervention. The study included N = 198 adolescents (Mage = 12.86 years, SDage = 0.75, 52% female, 41% immigrant descent, 49% intervention group) in Germany. Teacher-student relationships played an important role in facilitating intervention effects on identity development trajectories, emphasizing the importance of the relational context when implementing school-based interventions to promote heritage cultural identity development.
Lotus spp.: a Mediterranean genus with high environment and economic impact in the Salado River Basin (Argentina)
Authors: Ruiz Oscar Adolfo , Gortari Maximiliano , Maguire Vanina Giselle , Arese Romina Paola , Campestre María Paula , Antonelli Cristian Javier , Calzadilla Pablo Ignacio , Menéndez Ana Bernardina , Escaray Francisco José , Carrasco Sorli Pedro Miguel , Bailleres Matías Andrés , Ezquiaga Juan Pedro , Paolocci Francesco , Garriz Andrés , Nieva Amira Susana del Valle 
Published: May 2024

AbstractThis review has the principal objective in to hypothesise that the introduction of Lotus species could have significant benefits in constrained soils due their worldwide distribution. This idea is major based on results obtained in the lowlands of the most important livestock breeding area in Argentina denominated Salado River Basin (also called “Flooding Pampas”). Mostly of their land surface is dominated by salt-affected soils with severe constraints for traditional crop cultivation (i.e., maize, soybean, etc.). In order to increase their economic importance, farmers have utilized species such as non-native L. tenuis (ex- Lotus glaber), originating from European Mediterranean area, which shows a successfully and fast naturalization (in less than 60 years) in constrained areas improving forage performance. The increase in soil quality associated to this legume is achieved by an increment of the organic matter content and improvement of fertility and physicochemical parameters. Moreover, other studies have evidenced some genetic determinants associated with interesting agronomic traits such as plant tolerance to environmental stresses and the importance of leaves condensed tannins concentrations. This revision has many topics including a brief analysis of economic and environmental changes that occur under Lotus species implantation. In addition, we incorporate references recently published concerning the evaluation of the biochemical and physiological mechanisms involved in their adaptation to strong abiotic stresses characteristic of the region, the soil and plant microbiota diversity and soil physical and chemical characteristics associated to the presence of Lotus genotypes.