bluuebunny/crossref_metadata_2025_split huggingface dataset:
Various techniques for resolving overlapping ultraviolet spectra of combination pharmaceutical dosage forms containing hydroxychloroquine and paracetamol
Authors: Elbaramawi Samar S. , El-Adl Sobhy M. , Nafea Alaa , Mattar Amr A. , Sebaiy Mahmoud M. 
Published: May 2024

AbstractTen novel spectrophotometric approaches were developed for the initial examination of the Hydroxychloroquine and Paracetamol medications. These procedures are straightforward, specific, easy to use, and provide exact and accurate results. The determination was conducted through the utilization of several approaches, including zero order (dual wavelength, zero crossing, advanced absorption subtraction and spectrum subtraction), derivative (first derivative of zero crossing), ratio (ratio difference, ratio derivative) and mathematical (bivariate, simultaneous equation, and Q-absorbance) techniques. After undergoing validation in accordance with ICH criteria, it was established that each of these methods achieved acceptable levels of precision, repeatability, robustness, and accuracy. The advantages and disadvantages of each method are demonstrated, and the proposed and reported methodologies were statistically compared.
Graphical Abstract
Three-dimensional reconstruction of renal tumor anatomy for preoperative planning of robotic partial nephrectomy in renal cell carcinoma cases with duplex kidney: a case report
Authors: Nguyen Tuan Thanh , Thai Minh Sam , Chau Quy Thuan , Dobbs Ryan W. , Tiong Ho Yee , Pham Duc Minh , Truong Ho Trong Tan , Thai Kinh Luan , Nguyen Huynh Dang Khoa , Huynh Thanh Thien , Le Huu Phuoc , Ngo Xuan Thai 
Published: May 2024

Abstract
Background
The duplex kidney is one of the common congenital anomalies of the kidney and urinary tract. We present two cases of renal tumor accompanied with ipsilateral duplex kidney. The image of the tumor, renal artery system and collecting system were rendered by AI software (Fujifilm’s Synapse® AI Platform) to support the diagnosis and surgical planning.

Case presentation
Two Vietnamese patients (a 45-year-old man and a 54-year-old woman) with incidental cT1 renal cell carcinoma (RCC) were confirmed to have ipsilateral duplex kidneys by 3D reconstruction AI technique. One patient had a Renal score 9ah tumor of left kidney while the other had a Renal score 9 × tumor of right kidney in which a preoperative CT scan failed to identify a diagnosis of duplex kidney. Using the Da Vinci platform, we successfully performed robotic partial nephrectomy without any damage to the collecting system in both cases.

Conclusion
RCC with duplex kidneys is a rare condition. By utilizing a novel AI reconstruction technique with adequate information, two patients with RCC in duplex kidneys were successfully performed robotic partial nephrectomy without complication.
Pseudorabies virus hijacks the Rab6 protein to promote viral assembly and egress
Authors: Liang Dong-Ge , Guo Yu-Kun , Zhao Shi-Bo , Yang Guo-Yu , Han Ying-Qian , Chu Bei-Bei , Ming Sheng-Li 
Published: May 2024

AbstractPseudorabies virus (PRV) is recognized as the aetiological agent responsible for Aujeszky’s disease, or pseudorabies, in swine populations. Rab6, a member of the small GTPase family, is implicated in various membrane trafficking processes, particularly exocytosis regulation. Its involvement in PRV infection, however, has not been documented previously. In our study, we observed a significant increase in the Rab6 mRNA and protein levels in both PK-15 porcine kidney epithelial cells and porcine alveolar macrophages, as well as in the lungs and spleens of mice infected with PRV. The overexpression of wild-type Rab6 and its GTP-bound mutant facilitated PRV proliferation, whereas the GDP-bound mutant form of Rab6 had no effect on viral propagation. These findings indicated that the GTPase activity of Rab6 was crucial for the successful spread of PRV. Further investigations revealed that the reduction in Rab6 levels through knockdown significantly hampered PRV proliferation and disrupted virus assembly and egress. At the molecular level, Rab6 was found to interact with the PRV glycoproteins gB and gE, both of which are essential for viral assembly and egress. Our results collectively suggest that PRV exploits Rab6 to expedite its assembly and egress and identify Rab6 as a promising novel target for therapeutic treatment for PRV infection.
Optimizing feature fusion for improved zero-shot adaptation in text-to-speech synthesis
Authors: Chen Zhiyong , Ai Zhiqi , Ma Youxuan , Li Xinnuo , Xu Shugong 
Published: May 2024

AbstractIn the era of advanced text-to-speech (TTS) systems capable of generating high-fidelity, human-like speech by referring a reference speech, voice cloning (VC), or zero-shot TTS (ZS-TTS), stands out as an important subtask. A primary challenge in VC is maintaining speech quality and speaker similarity with limited reference data for a specific speaker. However, existing VC systems often rely on naive combinations of embedded speaker vectors for speaker control, which compromises the capture of speaking style, voice print, and semantic accuracy. To overcome this, we introduce the Two-branch Speaker Control Module (TSCM), a novel and highly adaptable voice cloning module designed to precisely processing speaker or style control for a target speaker. Our method uses an advanced fusion of local-level features from a Gated Convolutional Network (GCN) and utterance-level features from a gated recurrent unit (GRU) to enhance speaker control. We demonstrate the effectiveness of TSCM by integrating it into advanced TTS systems like FastSpeech 2 and VITS architectures, significantly optimizing their performance. Experimental results show that TSCM enables accurate voice cloning for a target speaker with minimal data through both zero-shot or few-shot fine-tuning of pretrained TTS models. Furthermore, our TSCM-based VITS (TSCM-VITS) showcases superior performance in zero-shot scenarios compared to existing state-of-the-art VC systems, even with basic dataset configurations. Our method’s superiority is validated through comprehensive subjective and objective evaluations. A demonstration of our system is available athttps://great-research.github.io/tsct-tts-demo/, providing practical insights into its application and effectiveness.
Occurrence and inhalation health risk of neonicotinoid pesticides in outdoor air particulate matters from 2019 to 2021 in China
Authors: Du Hairong , Zhu Xiaoling , Yao Yunying , Yao Wei 
Published: May 2024

AbstractNeonicotinoid insecticides (NNIs), as a new type of insecticide, are widely used in agriculture and daily life. Because of the low volatility of NNIs, few studies have evaluated them in atmospheric particulate matter. In this study, 101 outdoor PM2.5 samples were collected from the Wuhan urban area from 2019 to 2021, leading to the detection of seven NNIs and three of their metabolites. The detection frequencies of all 10 substances were more than 60%, with DIN (dinotefuran) and IMI (imidacloprid) reaching 100%. DIN (52.4 pg/m3) and IMI (43.0 pg/m3) had higher median concentrations than other substances. Concentration distributions of the four substances ACE (acetamiprid), DIN, IMI, and 5-OH-IMI (5-Hydro-Imidacloprid) exhibited statistically significant differences (P < 0.05) across the four seasons. Concentration levels of ACE, CLO (clothianidin), and IMI were statistically different between the three years (P < 0.05). The median concentration of imidacloprid-equivalent total neonicotinoids (IMIeq: generated by the relative potency factor method) was 256.1 pg/m3. Finally, the estimated daily intake (EDI) of NNIs via respiration was greater in infants and young children than in the rest of the population, suggesting that infants and young children were more likely to be exposed to the health effects of airborne residual PM2.5.
MEM&SO protocol: understanding the determinants of social learning in neurodegenerative diseases
Authors: Saliou Pauline , Chavant Julien , Belliard Serge , Merck Catherine , de La Sayette Vincent , Wallon David , Martinaud Olivier , Eustache Francis , Laisney Mickaël 
Published: May 2024

Abstract
Background
People with neurodegenerative diseases may have difficulty learning new information, owing to their cognitive impairments. Teaching them techniques for learning in social contexts could alleviate this difficulty. The present study will examine the performances of patients with Alzheimer’s disease and patients with the semantic variant of primary progressive aphasia on a memory test administered in three social contexts. The protocol will make it possible to identify determinants of social interactions, social abilities, cognition, and personality that can explain the potentially beneficial effect of social context on learning in these patients.

Methods
Thirty dyads (patient with primary memory impairment who meets criteria for Alzheimer’s disease paired with caregiver), 16 dyads (patient meeting criteria for semantic variant of primary progressive aphasia paired with caregiver), and 46 dyads (healthy controls with no cognitive complaints) will be recruited. A nonverbal memory test (social memory task) will be administered to each dyad in three different social contexts (presence-only, observation, collaboration). Patients and healthy controls will also undergo a neuropsychological assessment to measure social (interactions and abilities), cognitive and personality aspects. Patients will be compared with controls on differential social scores calculated between the presence-only and collaboration contexts, and between the presence-only and observation contexts. A multiple comparative case study will be conducted to identify social, cognitive and personality variables that potentially explain the differential scores in the collaboration and observation contexts.

Discussion
For the first time, memory will be assessed in patients with Alzheimer’s disease and patients with the semantic variant of primary progressive aphasia in three different contexts (presence-only, observation, collaboration). The multiple comparative case study will make it possible to identify the determinants of memory performance in the social context, in order to create the most beneficial learning context for individual patients, according to their profile.

Trial registration
This study was approved by the Ile de France XI institutional review board (2022-A00198-35), and registered on ClinicalTrials.gov (no. NCT05800028), on April 27, 2023.
The impact of the Hunga Tonga–Hunga Ha’apai volcanic eruption on the Peruvian atmosphere: from the sea surface to the ionosphere
Authors: Pacheco E. E. , Velasquez J. P. , Flores R. , Condori L. , Fajardo G. , Kuyeng K. , Scipion D. E. , Milla M. , Conte J. F. , Poblet F. L. , Chau J. L. , Suclupe J. , Rojas R. , Manay E. 
Published: May 2024

AbstractThe eruption of the Hunga Tonga Hunga Ha’apai volcano on 15 January 2022 significantly impacted the lower and upper atmosphere globally. Using multi-instrument observations, we described disturbances from the sea surface to the ionosphere associated with atmospheric waves generated by the volcanic eruption. Perturbations were detected in atmospheric pressure, horizontal magnetic field, equatorial electrojet (EEJ), ionospheric plasma drifts, total electron content (TEC), mesospheric and lower thermospheric (MLT) neutral winds, and ionospheric virtual height measured at low magnetic latitudes in the western South American sector (mainly in Peru). The eastward Lamb wave propagation was observed at the Jicamarca Radio Observatory on the day of the eruption at 13:50 UT and on its way back from the antipodal point (westward) on the next day at 07:05 UT. Perturbations in the horizontal component of the magnetic field (indicative of EEJ variations) were detected between 12:00 and 22:00 UT. During the same period, GNSS-TEC measurements of traveling ionospheric disturbances (TIDs) coincided approximately with the arrival time of Lamb and tsunami waves. On the other hand, a large westward variation of MLT winds occurred near 18:00 UT over Peru. However, MLT perturbations due to possible westward waves from the antipode have not been identified. In addition, daytime vertical plasma drifts showed an unusual downward behavior between 12:00 and 16:00 UT, followed by an upward enhancement between 16:00 and 19:00 UT. Untypical daytime eastward zonal plasma drifts were observed when westward drifts were expected. Variations in the EEJ are highly correlated with perturbations in the vertical plasma drift exhibiting a counter-equatorial electrojet (CEEJ) between 12:00 and 16:00 UT. These observations of plasma drifts and EEJ are, so far, the only ground-based radar measurements of these parameters in the western South American region after the eruption. We attributed the ion drift and EEJ perturbations to large-scale thermospheric wind variations produced by the eruption, which altered the dynamo electric field in the Hall and Pedersen regions. These types of multiple and simultaneous observations can contribute to advancing our understanding of the ionospheric processes associated with natural hazard events and the interaction with lower atmospheric layers.
Graphical Abstract
Leaving no one behind in armed conflict-affected settings of Africa: is universal health coverage a possibility or mirage?
Authors: Olu Olushayo Oluseun , Petu Amos , Usman Abdulmumini 
Published: May 2024

AbstractThe world is off track six years to the 2030 deadline for attaining the sustainable development goals and universal health coverage. This is particularly evident in Africa’s armed conflict-affected and humanitarian settings, where pervasively weak health systems, extreme poverty and inequitable access to the social dimensions and other determinants of health continue to pose significant challenges to universal health coverage. In this article, we review the key issues and main barriers to universal health coverage in such settings. While our review shows that the current health service delivery and financing models in Africa’s armed conflict-affected settings provide some opportunities to leapfrog progress, others are threats which could hinder the attainment of universal health coverage. We propose four key approaches focused on addressing the barriers to the three pillars of universal health coverage, strengthening public disaster risk management, bridging the humanitarian-development divide, and using health as an enabler of peace and sustainable development as panacea to addressing the universal health coverage challenge in these settings. The principles of health system strengthening, primary health care, equity, the right to health, and gender mainstreaming should underscore the implementation of these approaches. Moving forward, we call for more advocacy, dialogue, and research to better define and adapt these approaches into a realistic package of interventions for attaining universal health coverage in Africa’s armed conflict-affected settings.
Surgical outcome of isolated benign peripheral nerve sheath tumors without neurofibromatosis
Authors: Salim Mohammud Ahmud , Elnoamany Hossam , Dorrah Mohammed Adel , Mahdy Zahraa M. , Mansour Ahmed Said 
Published: May 2024

Abstract
Background
Benign peripheral nerve sheath tumors (BPNSTs) include schwannomas and neurofibromas. About 10% of soft tissue sarcomas are malignant peripheral nerve sheath tumors (MPNSTs), which are invasive and aggressive tumors. These can happen occasionally or after radiation exposure. Up to 90% of schwannomas are made up of differentiated neoplastic Schwann cells. Malignant transformation of schwannomas is rare.

Methods
We collected the medical records of all patients (including their family histories), performed comprehensive physical and neurological assessments, and checked for the presence of a Tinel-like sign, as well as screening for neurofibromatosis (NF) signs. Magnetic resonance imaging (MRI), nerve conduction studies, and ultrasound were done for all cases.

Results
We have operated on 21 patients with age range 29–52 years. The mean age was 39.4 years. 14 of these patients were females and 7 were males. The presenting symptoms were just swelling at nerve site in 14 patients, spontaneous pain at the nerve sites in 7 patients, and sensory deficit at the nerve distribution sites in 9 patients. The other 12 patients were sensory intact and only 7 patients had motor deficit. Postoperatively all patients had improved motor and sensory deficit and none of intact patients were worsened. Preoperatively we did MRI to show important nearby vascular structure anatomical abnormalities and we ordered nerve studies to all patients that showed abnormalities, which was only in 9 patients. Gross total resection was done in 18 patients and other 3 cases had partial resection to avoid sensory and motor deficits. The pathological analysis revealed 11 schwannomas and 10 neurofibromas. With 1 year follow up there was no recurrence in any patients.

Conclusion
Benign pheripheral nerve sheath tumours are safely resected without increased sensory and motor deficits after surgery and with improve clinical outcome with no recurrence on follow up.
Assessment of development and consumption rate of Macrolophus caliginosus Wagner predator on different prey stages
Authors: Sadek Hanaa E. , Ebadah Ibrahim M. A. , Moawad Sawsan S. 
Published: May 2024

Abstract
Background
Macrolophus caliginosus Wagner is one of the most important polyphagous predatory mirid bug that attack many agriculture insect pests.

Methods
The present study was carried out to show the effect of different prey stages as Tuta absoluta (eggs and fourth larval stage), Bemisia tabaci (eggs and three nymph stages) and Aphids gossypii (1st nymph stage) on the life cycle and consumption rate of male and females of M. caliginosus as predator under laboratory condition. The experiments were started by collecting the first stage of M. caliginosus nymph and placed separately on acalypha plants situated on saturated cotton pads inside Petri dishes with an excess of each prey.

Results
The results showed that the developmental period of the total immature stages of M. caliginosus female are affected by type and stage of tested prey. So, the duration of larval stages of female was significantly recorded the longest in case of feeding on eggs of T. absoluta compare to 1st nymph of A. gossypii. In addition to extension the adult longevity of female M. caliginosus, which fed on the 1st nymphal instar of B. tabaci more than the other pests. The consumption rate of total immature stages and adult M. caliginosus female were fed on eggs of B. tabaci was more than the other test pests. While the consumption rate of total immature stages and adult of male M. caliginosus were fed on eggs of B. tabaci was higher than that of the other pests.

Conclusions
The search was given the highlight focus on ability of mirid bugs to consume different insect species “stages” that might be facilitate rear predatory bugs under laboratory condition.
Drowsiness detection in real-time via convolutional neural networks and transfer learning
Authors: Salem Dina , Waleed Mohamed 
Published: May 2024

AbstractDrowsiness detection is a critical aspect of ensuring safety in various domains, including transportation, online learning, and multimedia consumption. This research paper presents a comprehensive investigation into drowsiness detection methods, with a specific focus on utilizing convolutional neural networks (CNN) and transfer learning. Notably, the proposed study extends beyond theoretical exploration to practical application, as we have developed a user-friendly mobile application incorporating these advanced techniques. Diverse datasets are integrated to systematically evaluate the implemented model, and the results showcase its remarkable effectiveness. For both multi-class and binary classification scenarios, our drowsiness detection system achieves impressive accuracy rates ranging from 90 to 99.86%. This research not only contributes to the academic understanding of drowsiness detection but also highlights the successful implementation of such methodologies in real-world scenarios through the development of our application.
ATP1A3 regulates protein synthesis for mitochondrial stability under heat stress
Authors: Fujii Fumihiko , Kanemasa Hikaru , Okuzono Sayaka , Setoyama Daiki , Taira Ryoji , Yonemoto Kousuke , Motomura Yoshitomo , Kato Hiroki , Masuda Keiji , Kato Takahiro A. , Ohga Shouichi , Sakai Yasunari 
Published: May 2024

ABSTRACT
Pathogenic variants in ATP1A3, the gene encoding the α3 subunit of the Na+/K+-ATPase, cause alternating hemiplegia of childhood (AHC) and related disorders. Impairments in Na+/K+-ATPase activity are associated with the clinical phenotype. However, it remains unclear whether additional mechanisms are involved in the exaggerated symptoms under stressed conditions in patients with AHC. We herein report that the intracellular loop (ICL) of ATP1A3 interacted with RNA-binding proteins, such as Eif4g (encoded by Eif4g1), Pabpc1 and Fmrp (encoded by Fmr1), in mouse Neuro2a cells. Both the siRNA-mediated depletion of Atp1a3 and ectopic expression of the p.R756C variant of human ATP1A3-ICL in Neuro2a cells resulted in excessive phosphorylation of ribosomal protein S6 (encoded by Rps6) and increased susceptibility to heat stress. In agreement with these findings, induced pluripotent stem cells (iPSCs) from a patient with the p.R756C variant were more vulnerable to heat stress than control iPSCs. Neurons established from the patient-derived iPSCs showed lower calcium influxes in responses to stimulation with ATP than those in control iPSCs. These data indicate that inefficient protein synthesis contributes to the progressive and deteriorating phenotypes in patients with the p.R756C variant among a variety of ATP1A3-related disorders.
Taf1 knockout is lethal in embryonic male mice and heterozygous females show weight and movement disorders
Authors: Crombie Elisa M. , Korecki Andrea J. , Cleverley Karen , Adair Bethany A. , Cunningham Thomas J. , Lee Weaverly Colleen , Lengyell Tess C. , Maduro Cheryl , Mo Victor , Slade Liam M. , Zouhair Ines , Fisher Elizabeth M. C. , Simpson Elizabeth M. 
Published: May 2024

ABSTRACT
The TATA box-binding protein-associated factor 1 (TAF1) is a ubiquitously expressed protein and the largest subunit of the basal transcription factor TFIID, which plays a key role in initiation of RNA polymerase II-dependent transcription. TAF1 missense variants in human males cause X-linked intellectual disability, a neurodevelopmental disorder, and TAF1 is dysregulated in X-linked dystonia–parkinsonism, a neurodegenerative disorder. However, this field has lacked a genetic mouse model of TAF1 disease to explore its mechanism in mammals and treatments. Here, we generated and validated a conditional cre-lox allele and the first ubiquitous Taf1 knockout mouse. We discovered that Taf1 deletion in male mice was embryonically lethal, which may explain why no null variants have been identified in humans. In the brains of Taf1 heterozygous female mice, no differences were found in gross structure, overall expression and protein localisation, suggesting extreme skewed X inactivation towards the non-mutant chromosome. Nevertheless, these female mice exhibited a significant increase in weight, weight with age, and reduced movement, suggesting that a small subset of neurons was negatively impacted by Taf1 loss. Finally, this new mouse model may be a future platform for the development of TAF1 disease therapeutics.