bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of metabolic comorbidity on the association between body mass index and health-related quality of life: a Scotland-wide cross-sectional study of 5,608 participants
Authors: Ul-Haq Zia , Mackay Daniel F , Fenwick Elisabeth , Pell Jill P 
Published: February 2012

Abstract

Background
The prevalence of obesity is rising in Scotland and globally. Overall, obesity is associated with increased morbidity, mortality and reduced health-related quality of life. Studies suggest that "healthy obesity" (obesity without metabolic comorbidity) may not be associated with morbidity or mortality. Its impact on health-related quality of life is unknown.

Methods
We extracted data from the Scottish Health Survey on self-reported health-related quality of life, body mass index (BMI), demographic information and comorbidity. SF-12 responses were converted into an overall health utility score. Linear regression analyses were used to explore the association between BMI and health utility, stratified by the presence or absence of metabolic comorbidity (diabetes, hypertension, hypercholesterolemia or cardiovascular disease), and adjusted for potential confounders (age, sex and deprivation quintile).

Results
Of the 5,608 individuals, 3,744 (66.8%) were either overweight or obese and 921 (16.4%) had metabolic comorbidity. There was an inverted U-shaped relationship whereby health utility was highest among overweight individuals and fell with increasing BMI. There was a significant interaction with metabolic comorbidity (p = 0.007). Individuals with metabolic comorbidty had lower utility scores and a steeper decline in utility with increasing BMI (morbidly obese, adjusted coefficient: -0.064, 95% CI -0.115, -0.012, p = 0.015 for metabolic comorbidity versus -0.042, 95% CI -0.067, -0.018, p = 0.001 for no metabolic comorbidity).

Conclusions
The adverse impact of obesity on health-related quality of life is greater among individuals with metabolic comorbidity. However, increased BMI is associated with reduced health-related quality of life even in the absence of metabolic comorbidity, casting doubt on the notion of "healthy obesity".
Absolute lymphocyte count is associated with survival in ovarian cancer independent of tumor-infiltrating lymphocytes
Authors: Milne Katy , Alexander Cheryl , Webb John R , Sun Winnie , Dillon Kristy , Kalloger Steve E , Gilks C Blake , Clarke Blaise , Köbel Martin , Nelson Brad H 
Published: February 2012

Abstract

Background
The immune system strongly influences outcome in patients with ovarian cancer. In particular, the absolute lymphocyte count in peripheral blood (ALC) and the presence of tumor-infiltrating lymphocytes (TIL) have each been associated with favourable prognosis. However, the mechanistic relationships between ALC, TIL and prognosis are poorly understood. We hypothesized that high ALC values might be associated with stronger tumor immunity as manifested by increased TIL, decreased tumor burden and longer survival.

Methods
ALC values were collected from patient records ≥ 2 years before, during or after primary treatment for high-grade serous ovarian cancer (HGSC). Lymphocyte subsets were assessed in peripheral blood by flow cytometry. CD8+ and CD20+ TIL were assessed by immunohistochemistry.

Results
Overall, patients had normal ALC values two or more years prior to diagnosis of HGSC. These values were not predictive of disease severity or survival upon subsequent development of HGSC. Rather, ALC declined upon development of HGSC in proportion to disease burden. This decline involved all lymphocyte subsets. ALC increased following surgery, remained stable during chemotherapy, but rarely recovered to pre-diagnostic levels. ALC values recorded at diagnosis did not correlate with CD8+ or CD20+ TIL but were associated with progression-free survival.

Conclusions
Patients with high intrinsic ALC values show no clinical or survival advantage upon subsequent development of HGSC. ALC values at diagnosis are prognostic due to an association with disease burden rather than TIL. Therapeutic enhancement of ALC may be necessary but not sufficient to improve survival in HGSC.
Development of an ELISA-array for simultaneous detection of five encephalitis viruses
Authors: Kang Xiaoping , Li Yuchang , Fan Li , Lin Fang , Wei Jingjing , Zhu Xiaolei , Hu Yi , Li Jing , Chang Guohui , Zhu Qingyu , Liu Hong , Yang Yinhui 
Published: February 2012

Abstract
Japanese encephalitis virus(JEV), tick-borne encephalitis virus(TBEV), and eastern equine encephalitis virus (EEEV) can cause symptoms of encephalitis. Establishment of accurate and easy methods by which to detect these viruses is essential for the prevention and treatment of associated infectious diseases. Currently, there are still no multiple antigen detection methods available clinically. An ELISA-array, which detects multiple antigens, is easy to handle, and inexpensive, has enormous potential in pathogen detection. An ELISA-array method for the simultaneous detection of five encephalitis viruses was developed in this study. Seven monoclonal antibodies against five encephalitis-associated viruses were prepared and used for development of the ELISA-array. The ELISA-array assay is based on a "sandwich" ELISA format and consists of viral antibodies printed directly on 96-well microtiter plates, allowing for direct detection of 5 viruses. The developed ELISA-array proved to have similar specificity and higher sensitivity compared with the conventional ELISAs. This method was validated by different viral cultures and three chicken eggs inoculated with infected patient serum. The results demonstrated that the developed ELISA-array is sensitive and easy to use, which would have potential for clinical use.
Minocycline Markedly Reduces Acute Visceral Nociception via Inhibiting Neuronal ERK Phosphorylation
Authors: Cho Ik-Hyun , Lee Min Jung , Jang Minhee , Gwak Nam Gil , Lee Ka Yeon , Jung Hyuk-Sang 
Published: February 2012

Background: Minocycline prevents the development of neuropathic and inflammatory pain by inhibiting microglial activation and postsynaptic currents. But, how minocycline obviates acute visceral pain is unclear. The present study investigated whether minocycline had an any antinociceptive effect on acetic acid-induced acute abdominal pain after intraperitoneal (i.p.) administration of saline or minocycline 1 hour before acetic acid injection (1.0%, 250 μl, i.p.). Results: Minocycline (4, 10, or 40 mg/kg) significantly decreased acetic acid-induced nociception (0–60 minutes post-injection) and the enhancement in the number of c-Fos positive cells in the T5-L2 spinal cord induced by acetic acid injection. Also, the expression of spinal phosphorylated extracellular signal-regulated kinase (p-ERK) induced by acetic acid was reduced by minocycline pre-administration. Interestingly, intrathecal introduction of PD98059, an ERK upstream kinase inhibitor, markedly blocked the acetic acid-stimulated pain responses. Conclusions: These results demonstrate that minocycline effectively inhibits acetic acid-induced acute abdominal nociception via the inhibition of neuronal p-ERK expression in the spinal cord, and that minocycline may have therapeutic potential in suppressing acute abdominal pain.
The Expression of Spinal Methyl-CpG-Binding Protein 2, DNA Methyltransferases and Histone Deacetylases is Modulated in Persistent Pain States
Authors: Tochiki Keri K , Cunningham Joel , Hunt Stephen P , Géranton Sandrine M 
Published: February 2012

Background: DNA CpG methylation is carried out by DNA methyltransferases and induces chromatin remodeling and gene silencing through a transcription repressor complex comprising the methyl-CpG-binding protein 2 (MeCP2) and a subset of histone deacetylases. Recently, we have found that MeCP2 activity had a crucial role in the pattern of gene expression seen in the superficial dorsal horn rapidly after injection of Complete Freund's Adjuvant (CFA) in the rat ankle joint. The aim of the present study was to analyse the changes in expression of MeCP2, DNA methyltransferases and a subset of histone deacetylases in the superficial dorsal horn during the maintenance phase of persistent pain states. In this process, the cell specific expression of MeCP2 was also investigated. Results: Using immunohistochemistry, we found that neurones, oligodendrocytes and astrocytes expressed MeCP2. Microglia, oligodendrocyte precursor cells and Schwann cells never showed any positive stain for MeCP2. Quantitative analyses showed that MeCP2 expression was increased in the superficial dorsal horn 7 days following CFA injection in the ankle joint but decreased 7 days following spared nerve injury. Overall, the expression of DNA methyltransferases and a subset of histone deacetylases followed the same pattern of expression. However, there were no significant changes in the expression of the MeCP2 targets that we had previously shown are regulated in the early time points following CFA injection in the ankle joint. Finally, the expression of MeCP2 was also down regulated in damaged dorsal root ganglion neurones following spared nerve injury. Conclusion: Our results strongly suggest that changes in chromatin compaction, regulated by the binding of MeCP2 complexes to methylated DNA, are involved in the modulation of gene expression in the superficial dorsal horn and dorsal root ganglia during the maintenance of persistent pain states.
The human side of influenza
Authors: Oshansky Christine M , Thomas Paul G 
Published: February 2012

Abstract
Review of immunity to influenza virus infection in humans, noting evidence from murine studies and suggesting directions in which future research is most needed.
A clear understanding of immunity in individuals infected with influenza virus is critical for the design of effective vaccination and treatment strategies. Whereas myriad studies have teased apart innate and adaptive immune responses to influenza infection in murine models, much less is known about human immunity as a result of the ethical and technical constraints of human research. Still, these murine studies have provided important insights into the critical correlates of protection and pathogenicity in human infection and helped direct the human studies that have been conducted. Here, we examine and review the current literature on immunity in humans infected with influenza virus, noting evidence offered by select murine studies and suggesting directions in which future research is most warranted.
Flow noise reduction from superhydrophobic surfaces
Authors: Elboth Thomas , Pettersson Reif Bjørn Anders , Andreassen Øyvind , Martell Michael B. 
Published: February 2012

This work investigates how a highly (super)hydrophobic surface can be used to reduce turbulence-generated drag and noise on a towed streamer cable. The work is done by analyzing full-scale drag and flow noise measurements taken on a commercial seismic streamer in combination with direct numerical simulations of turbulence-generated flow noise. The main findings are that viscous drag and flow noise can be significantly reduced on a seismic streamer that is coated to make the surface highly hydrophobic. In an ocean towing test, a 4% reduction of drag on a streamer section was measured. In a separate test on a commercial seismic vessel, a reduction in the flow noise level of nearly 50% (6 dB) for frequencies below 10 Hz was found. Based upon an analysis of numerical simulation data, it is suggested that the reduction in drag and noise can be attributed to a reduced level of shear stress and change in the kinematic structure of the turbulence, both of which occur in the immediate vicinity of the highly hydrophobic surface.
Msx1 is expressed in retina endothelial cells at artery branching sites
Authors: Lopes Miguel , Goupille Olivier , Saint Cloment Cécile , Robert Benoît 
Published: February 2012

Summary
Msx1 and Msx2 encode homeodomain transcription factors that play a role in several embryonic developmental processes. Previously, we have shown that in the adult mouse, Msx1lacZ is expressed in vascular smooth muscle cells (VSMCs) and pericytes, and that Msx2lacZ is also expressed in VSMCs as well as in a few endothelial cells (ECs). The mouse retina and choroid are two highly vascularized tissues. Vessel alterations in the retina are associated with several human diseases and the retina has been intensely used for angiogenesis studies, whereas the choroid has been much less investigated. Using the Msx1lacZ and Msx2lacZ reporter alleles, we observed that Msx2 is not expressed in the eye vascular tree in contrast to Msx1, for which we establish the spatial and temporal expression pattern in these tissues. In the retina, expression of Msx1 takes place from P3, and by P10, it becomes confined to a subpopulation of ECs at branching points of superficial arterioles. These branching sites are characterized by a subpopulation of mural cells that also show specific expression programs. Specific Msx gene inactivation in the endothelium, using Msx1 and Msx2 conditional mutant alleles together with a Tie2-Cre transgene, did not lead to conspicuous structural defects in the retinal vascular network. Expression of Msx1 at branching sites might therefore be linked to vessel physiology. The retinal blood flow is autonomously regulated and perfusion of capillaries has been proposed to depend on arteriolar precapillary structures that might be the sites for Msx1 expression. On the other hand, branching sites are subject to shear stress that might induce Msx1 expression. In the choroid vascular layer Msx1lacZ is expressed more broadly and dynamically. At birth Msx1lacZ expression takes place in the endothelium but at P21 its expression has shifted towards the mural layer. We discuss the possible functions of Msx1 in the eye vasculature.
A comprehensive analysis of reassortment in influenza A virus
Authors: de Silva U. Chandimal , Tanaka Hokuto , Nakamura Shota , Goto Naohisa , Yasunaga Teruo 
Published: February 2012

Summary
Genetic reassortment plays a vital role in the evolution of the influenza virus and has historically been linked with the emergence of pandemic strains. Reassortment is believed to occur when a single host - typically swine - is simultaneously infected with multiple influenza strains. The reassorted viral strains with novel gene combinations tend to easily evade the immune system in other host species, satisfying the basic requirements of a virus with pandemic potential. Therefore, it is vital to continuously monitor the genetic content of circulating influenza strains and keep an eye out for new reassortants. We present a new approach to identify reassortants from large data sets of influenza whole genome nucleotide sequences and report the results of the first ever comprehensive search for reassortants of all published influenza A genomic data. 35 of the 52 well supported candidate reassortants we found are reported here for the first time while our analysis method offers new insight that enables us to draw a more detailed picture of the origin of some of the previously reported reassortants. A disproportionately high number (13/52) of the candidate reassortants found were the result of the introduction of novel hemagglutinin and/or neuraminidase genes into a previously circulating virus. The method described in this paper may contribute towards automating the task of routinely searching for reassortants among newly sequenced strains.
Interactions between the amnioserosa and the epidermis revealed by the function of the u-shaped gene
Authors: Lada Karolina , Gorfinkiel Nicole , Martinez Arias Alfonso 
Published: February 2012

Summary
Dorsal closure (DC) is an essential step during Drosophila development whereby a hole is sealed in the dorsal epidermis and serves as a model for cell sheet morphogenesis and wound healing. It involves the orchestrated interplay of transcriptional networks and dynamic regulation of cell machinery to bring about shape changes, mechanical forces, and emergent properties. Here we provide insight into the regulation of dorsal closure by describing novel autonomous and non-autonomous roles for U-shaped (Ush) in the amnioserosa, the epidermis, and in mediation of communication between the tissues. We identified Ush by gene expression microarray analysis of Dpp signaling targets and show that Ush mediates some DC functions of Dpp. By selectively restoring Ush function in either the AS or the epidermis in ush mutants, we show that the AS makes a greater (Ush-dependent) contribution to closure than the epidermis. A signal from the AS induces epidermal cell elongation and JNK activation in the DME, while cable formation requires Ush on both sides of the leading edge, i.e. in both the AS and epidermis. Our study demonstrates that the amnioserosa and epidermis communicate at several steps during the process: sometimes the epidermis instructs the amnioserosa, other times the AS instructs the epidermis, and still other times they appear to collaborate.
NuRD-dependent DNA methylation prevents ES cells from accessing a trophectoderm fate
Authors: Latos Paulina A. , Helliwell Christine , Mosaku Olukunbi , Dudzinska Dominika A. , Stubbs Bryony , Berdasco Maria , Esteller Manel , Hendrich Brian 
Published: February 2012

Summary
Embryonic Stem (ES) cells are able to give rise to the three germ layers of the embryo but are prevented from contributing to the trophoblast. The molecular nature of this barrier between embryonic and trophectodermal cell fates is not clear, but is known to involve DNA methylation. Here we demonstrate that the Nucleosome Remodeling and Deacetylation (NuRD) co-repressor complex maintains the developmental barrier between embryonic and trophectodermal cell fates by maintaining transcriptional silencing of trophectoderm determinant genes in ES cells. We further show that NuRD activity facilitates DNA methylation of several of its target promoters, where it acts non-redundantly with DNA methylation to enforce transcriptional silencing. NuRD-deficient ES cells fail to completely silence expression of the trophectoderm determinant genes Elf5 and Eomes, but this alone is not sufficient to induce transdifferentiation towards the trophectoderm fate. Rather this leaves ES cells capable of activating expression of trophectoderm-specific genes in response to appropriate extracellular signals, enabling them to commit to a trophectodermal cell fate. Our findings clarify the molecular nature of the developmental barrier between the embryonic and trophoblast cell fates, and establish a role for NuRD activity in specifying sites for de novo DNA methylation.
Long-term results of conventional varus half-wedge proximal femoral osteotomy for the treatment of osteonecrosis of the femoral head
Authors: Ito H. , Tanino H. , Yamanaka Y. , Nakamura T. , Takahashi D. , Minami A. , Matsuno T. 
Published: February 2012

We have previously described the mid- to long-term results of conventional simple varus intertrochanteric osteotomy for osteonecrosis of the femoral head, showing that 19 of the 26 hips had good or excellent results. We extended the follow-up to a mean of 18.1 years (10.5 to 26) including a total of 34 hips in 28 patients, with a mean age at surgery of 33 years (19 to 53). There were 18 men and ten women and 25 hips (74%) had a satisfactory result with a Harris hip score ≥ 80. In all, six hips needed total hip replacement (THR) or hemiarthroplasty. The collapse of the femoral head or narrowing of the joint space was found to have progressed in nine hips (26%). Leg shortening after osteotomy was a mean of 19 mm (8 to 36). With conversion to THR or hemiarthroplasty as the endpoint, the ten-year survival rate was 88.2% (95% confidence interval (CI) 82.7 to 93.7) and the 20-year survival rate was 79.7% (95% CI 72.1 to 87.3); four hips were converted at ten years and other two hips were converted at 20 years.Shortening of the leg after osteotomy remains a concern; however, the conventional varus half-wedge osteotomy provides favourable long-term results in hips with less than two-thirds of the medial part of the femoral head affected by necrotic bone and with normal bone superolaterally.
Comparison of two sequential separation methods for U and Th determination in environmental samples by alpha-particle spectrometry
Authors: Lehritani M. , Mantero J. , Casacuberta N. , Masqué P. , García-Tenorio R. 
Published: February 2012

Abstract
This paper deals with radiochemical procedures for U and Th determination (238U, 234U, 232Th and 230Th) via alpha spectrometry in water and sediment samples. After checking a separation process based on chromatographic extraction (using UTEVA resins), a detailed comparison with another separation process (liquid–liquid solvent extraction using tri-n-butyl phosphate-TBP) has been performed. Both separation processes were applied in environmental liquid and solid samples and the two separation methods were compared in order to find the more suitable separation process attending on parameters such as chemical yields, interference elimination, time-consuming, reagents used and generated wastes, among others. As major result, we can indicate the UTEVA separation process as a better option than TBP in U and Th isotopes determination by alpha-particle spectrometry.
Permission to Dissent: Civil Religion and the Radio Western, 1933–1960
Authors: Wedel Kip Anthony 
Published: February 2012

AbstractRadio drama gave Americans a new form of commercial entertainment in the 1930s, but the stories themselves contained time-honored elements. One of these was the rhetorical tradition scholars have identified as American civil religion. Radio Westerns were particularly well suited to promulgate familiar civil religion themes. They described the United States as an instrument of divine will in history, celebrated Americans as pious people, and associated national expansion with the implementation of God's will.The Lone Rangerwas the most famous Western to articulate these themes. The show's writers consciously sought to create in their hero a “composite of all men who uphold the laws of God and man.” During its long broadcast history from 1933 to 1954, the show attracted a large audience and inspired publishing, film, and television ventures. By the late 1940s, however, owing in part to World War II and in part to the Cold War, some Americans on both sides of the microphone found the old formula unsatisfactory.Gunsmoke, which premiered in 1951, exemplified a second generation of radio Westerns. Though still civil religious, these Westerns located the United States' religious significance less in national triumph than in personal triumphs of its citizens. In doing so, they critiqued the earlier Westerns and shifted from what Martin E. Marty has called the “priestly” to the “prophetic” form of civil religion. Their impatience with the older Westerns' use of civil religion also paralleled theological critiques of the popular Christianity of the 1950s.