bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microanatomic studies to define predictive factors for the topography of periarticular erosion formation in inflammatory arthritis
Authors: McGonagle Dennis , Tan Ai Lyn , Møller Døhn Uffe , ØStergaard Mikkel , Benjamin Michael 
Published: March 2009

AbstractObjectiveThe microanatomic basis for formation of erosions in inflammatory arthritis is incompletely understood but is thought to be related to bare areas and the associated cartilage–synovium junction. The purpose of this study was to test the hypothesis that erosion‐prone sites are associated with microdamage in macroscopically normal joints.MethodsHistologic evaluation of erosion‐prone sites was performed on 20 collateral ligaments (CLs) from the metacarpophalangeal (MCP) and proximal interphalangeal (PIP) joints of 5 normal cadavers. In addition, the MCP joints (n = 17) and PIP joints (n = 3) of 20 patients with rheumatoid arthritis (RA) were assessed by computed tomography (CT) to ascertain whether the topography of erosion formation in patients with RA corresponded to the cadaveric findings.ResultsAbsence of a bare area was noted in cadaveric tissue at the periligamentous erosion‐prone regions, especially in the distal MCP joints and both distal and proximal PIP joints. Nevertheless, these sites exhibited soft‐tissue pathologic features and bony microdamage/cyst formation. Other significant findings included the presence of pannus without inflammatory changes in the regions in which a bare area was absent, and the replacement of bare area regions with fibrovascular synovial tissue in joints without inflammatory changes. The sites of cadaveric tissue microdamage corresponded to CT‐determined erosion formation in the MCP and PIP joints of patients with RA, in whom erosions adjacent to the CLs were more common than dorsal or volar erosions.ConclusionPeriarticular erosion formation may not necessarily depend on the presence of a bare area and has a propensity to occur adjacent to ligaments in which bone microdamage is common. These findings suggest that periligamentous locations prone to microdamage may critically influence the topography of erosion formation in inflammatory arthritis.
Patterning of diverse mammalian cell types in serum free medium with photoablation
Authors: Dhir Vipra , Natarajan Anupama , Stancescu Maria , Chunder Anindarupa , Bhargava Neelima , Das Mainak , Zhai Lei , Molnar Peter 
Published: March 2009

AbstractIntegration of living cells with novel microdevices requires the development of innovative technologies for manipulating cells. Chemical surface patterning has been proven as an effective method to control the attachment and growth of diverse cell populations. Patterning polyelectrolyte multilayers through the combination of layer‐by‐layer self‐assembly technique and photolithography offer a simple, versatile, and silicon compatible approach that overcomes chemical surface patterning limitations, such as short‐term stability and low‐protein adsorption resistance. In this study, direct photolithographic patterning of two types of multilayers, PAA (poly acrylic acid)/PAAm (poly acryl amide) and PAA/PAH (poly allyl amine hydrochloride), were developed to pattern mammalian neuronal, skeletal, and cardiac muscle cells. For all studied cell types, PAA/PAAm multilayers behaved as a cytophobic surface, completely preventing cell attachment. In contrast, PAA/PAH multilayers have shown a cell‐selective behavior, promoting the attachment and growth of neuronal cells (embryonic rat hippocampal and NG108‐15 cells) to a greater extent, while providing little attachment for neonatal rat cardiac and skeletal muscle cells (C2C12 cell line). PAA/PAAm multilayer cellular patterns have also shown a remarkable protein adsorption resistance. Protein adsorption protocols commonly used for surface treatment in cell culture did not compromise the cell attachment inhibiting feature of the PAA/PAAm multilayer patterns. The combination of polyelectrolyte multilayer patterns with different adsorbed proteins could expand the applicability of this technology to cell types that require specific proteins either on the surface or in the medium for attachment or differentiation, and could not be patterned using the traditional methods. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2009
Protein Iodination by Click Chemistry
Authors: Dong Shouliang , Moroder Luis , Budisa Nediljko 
Published: March 2009

AbstractTwo steps to click iodine: We have developed a two‐step reaction for protein iodination using click chemistry. With this method, which is summarized in the scheme, covalent attachment of a stable iodine‐containing aromatic azide moiety to an alkyne‐containing protein was achieved.magnified image
Thermally Stable Solids Based on Endohedrally Doped ZnS Clusters
Authors: Matxain Jon M. , Piris Mario , Lopez Xabier , Ugalde Jesus M. 
Published: March 2009

AbstractCaught in a cage! Bare Zn12S12 and endrohedral X§Zn12S12–Y§Zn12S12 in the square‐faces‐square orientation (X=Na, K (red); Y=Cl, Br (blue)) have been fully optimized. Ab initio quantum‐dynamics calculations further confirmed the thermal stability of the calculated structures, which are found to be zeolite‐shaped with a pore diameter of about 1 nm. These solids may be used as narrow‐gap semiconducting materials.magnified imageThe existence of inorganic, hollow, fullerene‐like ZnS clusters has been theoretically predicted and then recently confirmed experimentally. These clusters were observed to trap alkali metals and halogens because the ionization energies (IE) of alkali metals are very similar to the electron affinities (EA) of halogens. This opens the possibility of forming molecular solids composed of these fullerene building blocks because the energy released due to the difference between the IE and EA would be very small. Herein we have focused on assembling bare Zn12S12 and endohedral X@Zn12S12–Y@Zn12S12 dimers (X=Na, K; Y=Cl, Br) by considering the square‐faces‐square orientation of every two adjacent clusters, which leads to a fcc cubic crystal structure in the solid. The structures were fully optimized in all cases, and their thermal stability was confirmed by ab initio thermal molecular dynamics calculations. The optimum lattice parameter of the solids was found to be around 13.8 Å, which corresponds to distances of about 2.5 Å between monomers, which is typical of covalent ZnS bonds. The resulting solids are nanoporous materials similar to B12N12. Due to their nanoporous structure, these zeolite‐shaped solids could be used in heterogeneous catalysis and as storage materials and molecular sieves.
6,13‐Diethynyl‐5,7,12,14‐tetraazapentacene
Authors: Miao Shaobin , Appleton Anthony L. , Berger Nancy , Barlow Stephen , Marder Seth R. , Hardcastle Kenneth I. , Bunz Uwe H. F. 
Published: March 2009

AbstractA new relative of pentacene: The dialkynylated tetraazapentacene (see figure) was prepared by a two‐step synthesis from the corresponding quinone derivative. The heteroacene is an air‐stable, dark‐blue, crystalline material and is of great interest as a potential organic n‐electron‐transport material.magnified image
A Study of Photocatalytic Processes Involving the Degradation of the Organic Load and Amoxicillin in Hospital Wastewater
Authors: Martins Ayrton F. , Mayer Francieli , Confortin Eliza C. , Frank Carla da S. 
Published: March 2009

AbstractAn investigation was carried out into the treatment of wastewater from the University Hospital of Santa Maria (HUSM) by means of a heterogeneous photocatalytic process (HPP) and a photo‐Fenton process (PFP) using Response Surface Methodology (RSM) for maximizing the removal efficiency of the chemical oxygen demand, COD. The rates of COD reduction obtained by HPP and PFP were 44 and 64.6%, respectively. The toxicity (LC50 values) of the hospital wastewater (pre‐ and post‐treatment) were determined by means of the bioassay Artemia salina. During the treatment a toxicity inhibition of 43.5% was achieved by photo‐Fenton and 46.3% by TiO2 photocatalysis. Spiked samples of hospital effluent with amoxicillin were degraded by 100% after treatment by HPP for 30 min and 85% after treatment by PFP for 60 min, as measured by high performance liquid chromatography (HPLC). All the treatments were conducted on a bench scale using a batch recirculation 2 L Dewar‐like photoreactor provided with a light intensity of 401 W/m2, which was irradiated by a 125 W medium pressure mercury vapor lamp. Ferrioxalate actinometry solution was used to measure the photon flux (q = 1.71·10–5 ± 1.25·10–7). The photonic efficiency was 30.00 ± 0.67% for PFP and 19.02 ± 0.88% for HPP.
Site‐specific anti‐tumor immunity: Differences in DC function, TGF‐β production and numbers of intratumoral Foxp3+ Treg
Authors: Biollaz Gregoire , Bernasconi Luca , Cretton Christine , Püntener Ursula , Frei Karl , Fontana Adriano , Suter Tobias 
Published: March 2009

AbstractGliomas localized within the CNS are generally not rejected by the immune system despite being immunogenic. This failure of the immune system has been associated both with glioma‐derived immunosuppressive molecules and the immune‐privileged state of the CNS. However, the relative contribution of tumor location to the glioma‐mediated immunosuppression, as well as the immune mechanisms involved in the failure of glioma rejection are not fully defined. We report here that syngeneic GL261 gliomas growing either intracranially or subcutaneously in mice are infiltrated by DC and T cells. However, only subcutaneous gliomas elicit an effective anti‐tumor immune response. In contrast to DC infiltrating subcutaneously grown GL261 gliomas, tumor‐infiltrating DC from intracranial gliomas do not activate antigen‐dependent T‐cell proliferation in vitro. In addition, brain‐localized GL261 gliomas are characterized by significantly higher numbers of Foxp3+ Treg and higher levels of TGF‐β1 mRNA and protein expression when compared with GL261 gliomas in the skin. Our data show that gliomas in the CNS, but not in the skin, give rise to TGF‐β production and accumulation of both Treg and functionally impaired DC. Thus, not the tumor itself, but its location dictates the efficiency of the anti‐tumor immune response.
B‐cell‐activating factor expressions in salivary epithelial cells after dsRNA virus infection depends on RNA‐activated protein kinase activation
Authors: Ittah Marc , Miceli‐Richard Corinne , Gottenberg Jacques‐Eric , Sellam Jérémie , Lepajolec Christine , Mariette Xavier 
Published: March 2009

AbstractB‐cell‐activating factor (BAFF) plays a key role in promoting activation of autoimmune B cells. This cytokine may be expressed in and secreted by salivary gland epithelial cells (SGEC) after stimulation with type I IFN or viral or synthetic dsRNA. Because this BAFF expression depends only in part on endosomal TLR and type I IFN, we investigated whether other dsRNA sensors could be implicated in BAFF expression. Using human SGEC, we confirmed the partial dependence of BAFF expression on TLR‐3 by replicating the partial inhibition of BAFF expression observed upon endosomal inhibition using TLR‐3 or Toll/IL‐1R domain‐containing protein inducing IFN‐β silencing mRNA, but not with TLR‐7 silencing mRNA. Melanoma differentiation‐associated gene 5 silencing mRNA had no effect on BAFF expression, but retinoic acid‐inducible gene I silencing mRNA had a slight effect observed following infection with dsRNA reovirus‐1. Inhibition of RNA‐activated protein kinase (PKR) by 2‐aminopurine completely abolished both BAFF mRNA and protein production after reovirus‐1 infection and poly(I:C) stimulation through NF‐κB and p38 MAPK pathways, with the latter implicated only after poly(I:C) stimulation. Thus, PKR is the dsRNA sensor implicated in BAFF induction in SGEC after dsRNA stimulation. In autoimmune diseases, PKR may be an interesting target for preventing BAFF following the induction of innate immunity.
Identification and characterization of survivin‐derived H‐2Kb‐restricted CTL epitopes
Authors: Hofmann Uta B. , Voigt Heike , Andersen Mads H. , Straten Per thor , Becker Jürgen C. , Eggert Andreas O. 
Published: March 2009

AbstractSurvivin is overexpressed in several malignancies and in tumor‐associated endothelium making it an attractive target for therapeutic cytotoxic T‐cell responses. Thus, it would be important to test this notion in preclinical models. Consequently, we screened the murine survivin sequence for potential binding Kb‐restricted octamer peptide epitopes. Two epitopes, which bind strongly to Kb, were selected to test their immunogenicity in vivo. Spleen cells from mice vaccinated by intradermal injection of mature DC pulsed with these peptides displayed reactivity to the respective epitopes. The natural processing and presentation of these epitopes by tumor cells was evident by the killing of murine melanoma cells by vaccination‐induced T cells. Subcutaneous challenge with syngeneic melanoma demonstrated the protective immunity of this vaccination. Notably, analysis of the vessel density in subcutaneous tumors revealed that survivin‐specific vaccination significantly reduced the number of intratumoral vessels. In summary, we demonstrated the immunogenicity of two Kb‐restricted peptide epitopes derived from the murine survivin protein; moreover, survivin‐specific vaccination not only resulted in a reduction of tumor cells but also the tumor supplying blood vessels. The presented preclinical model for survivin‐directed vaccination may serve as a valuable tool to improve already running clinical trials in a syngeneic tumor model.
Synthesis of Trinuclear, Dinuclear and Mononuclear Carbamato‐Zinc Complexes from Tetranuclear Precursors: A Top‐Down Synthetic Approach to New Carbamates
Authors: Domide Dan , Neuhäuser Christiane , Kaifer Elisabeth , Wadepohl Hubert , Himmel Hans‐Jörg 
Published: March 2009

AbstractIn this work we report on the synthesis of new mono‐, bi‐ and trinuclear carbamato‐Zn complexes by reaction of the tetranuclear precursor [ZnEt(O2CNR2)]4 (R = iPr and iBu) with the nitrogen bases pyridine, N,N,N′,N′‐tetramethylguanidine (tmg) and bis(N,N,N′,N′‐tetramethylguanidino)naphthalene (btmgn). The only reaction product obtained with pyridine is the dinuclear complex [(py)ZnEt(O2CNR2)]2. In the case of reaction of [ZnEt(O2CNR2)]4 with tmg, the dinuclear species [(tmg)ZnEt(O2CNR2)]2 (for molar ratios of 1:4) and the mononuclear bis‐carbamato complex [(tmg)2Zn(O2CNR2)2] (for molar ratios of 1:8) were obtained. Finally, the mono‐ and trinuclear carbamates [(btmgn)ZnEt(O2CNR2)] and [(btmgn)Zn3Et3(O2CNR2)3] were formed in the reaction between [ZnEt(O2CNR2)]4 and btmgn (molar ratios 1:4 and 1:2, respectively). All products were structurally characterized by X‐ray diffraction. Using this top‐down approach the synthesis of a variety of oligonuclear carbamato‐Zn complexes becomes possible. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Evaluation of natural and synthetic compounds according to their antioxidant activity using a multivariate approach
Authors: Capitani Caroline D. , Carvalho Ana Carolina L. , Rivelli Diogo P. , Barros Silvia B. M. , Castro Inar A. 
Published: March 2009

AbstractThe antioxidant activity of natural and synthetic compounds was evaluated using five in vitro methods: ferric reducing/antioxidant power (FRAP), 2,2‐diphenyl‐1‐picrylhydradzyl (DPPH), oxygen radical absorption capacity (ORAC), oxidation of an aqueous dispersion of linoleic acid accelerated by azo‐initiators (LAOX), and oxidation of a meat homogenate submitted to a thermal treatment (TBARS). All results were expressed as Trolox equivalents. The application of multivariate statistical techniques suggested that the phenolic compounds (caffeic acid, carnosic acid, genistein and resveratrol), beyond their high antioxidant activity measured by the DPPH, FRAP and TBARS methods, showed the highest ability to react with the radicals in the ORAC methodology, compared to the other compounds evaluated in this study (ascorbic acid, erythorbate, tocopherol, BHT, Trolox, tryptophan, citric acid, EDTA, glutathione, lecithin, methionine and tyrosine). This property was significantly correlated with the number of phenolic rings and catecholic structure present in the molecule. Based on a multivariate analysis, it is possible to select compounds from different clusters and explore their antioxidant activity interactions in food products.
WR1065 mitigates AZT‐ddI‐induced mutagenesis and inhibits viral replication
Authors: Walker Dale M. , Kajon Adriana E. , Torres Salina M. , Carter Meghan M. , McCash Consuelo L. , Swenberg James A. , Upton Patricia B. , Hardy Andrew W. , Olivero Ofelia A. , Shearer Gene M. , Poirier Miriam C. , Walker Vernon E. 
Published: March 2009

AbstractThe success of nucleoside reverse transcriptase inhibitors (NRTIs) in treating HIV‐1 infection and reducing mother‐to‐child transmission of the virus during pregnancy is accompanied by evidence that NRTIs cause long‐term health risks for cancer and mitochondrial disease. Thus, agents that mitigate toxicities of the current combination drug therapies are needed. Previous work had shown that the NRTI‐drug pair zidovudine (AZT)–didanosine (ddI) was highly cytotoxic and mutagenic; thus, we conducted preliminary studies to investigate the ability of the active moiety of amifostine, WR1065, to protect against the deleterious effects of this NRTI‐drug pair. In TK6 cells exposed to 100 μM AZT‐ddI (equimolar) for 3 days with or without 150 μM WR1065, WR1065 enhanced long‐term cell survival and significantly reduced AZT‐ddI‐induced mutations. Follow‐up studies were conducted to determine if coexposure to AZT and WR1065 abrogated the antiretroviral efficacy of AZT. In human T‐cell blasts infected with HIV‐1 in culture, inhibition of p24 protein production was observed in cells treated with 10 μM AZT in the absence or presence of 5–1,000 μM WR1065. Surprisingly, WR1065 alone exhibited dose‐related inhibition of HIV‐1 p24 protein production. WR1065 also had antiviral efficacy against three species of adenovirus and influenza A and B. Intracellular levels of unbound WR1065 were measured following in vitro/in vivo drug exposure. These pilot study results indicate that WR1065, at low intracellular levels, has cytoprotective and antimutagenic activities against the most mutagenic pair of NRTIs and has broad spectrum antiviral effects. These findings suggest that the activities have a possible common mode of action that merits further investigation. Environ. Mol. Mutagen. 2009. © 2009 Wiley‐Liss, Inc.
The effect of attentional training on body dissatisfaction and dietary restriction
Authors: Smith Evelyn , Rieger Elizabeth 
Published: March 2009

AbstractThe aim of the present study is to investigate the effect of attentional training towards shape, weight and food related information on body dissatisfaction and dietary restriction. A total of 98 female participants were trained to attend to negative shape/weight words, positive shape/weight words, negative (high calorie) food words, positive (low calorie) food words or neutral words. Subsequently, a body image challenge was presented and participants' body dissatisfaction and dietary restriction were measured. Results indicated that negative shape/weight attentional biases exacerbated body dissatisfaction and a bias towards negative food words intensified dietary restriction. The study provides evidence for specificity in the effects of attentional biases and supports the notion that attentional training may be a useful component in interventions to improve body image and reduce dieting. Copyright © 2009 John Wiley & Sons, Ltd and Eating Disorders Association.
System reduction strategy for Galerkin models of fluid flows
Authors: Noack Bernd R. , Schlegel Michael , Morzyński Marek , Tadmor Gilead 
Published: March 2009

AbstractWe propose a system reduction strategy for spectral and Galerkin models of incompressible fluid flows. This approach leads to dynamic models of lower order, based on a partition in slow, dominant and fast modes. In the reduced models, slow dynamics are incorporated as non‐linear manifold consistent with mean‐field theory. Fast dynamics are stochastically treated and can be lumped in eddy‐viscosity approaches. The employed interaction models between slow, dominant and fast dynamics respect momentum and energy balance equations in a mathematically rigorous manner—unlike unsteady Reynolds‐averaged Navier–Stokes models or Smagorinsky‐type reductions of the Navier–Stokes equation. The proposed system reduction strategy is employed to the cylinder wake benchmark. Copyright © 2009 John Wiley & Sons, Ltd.
KHSV− EBV− post‐transplant effusion lymphoma with plasmablastic features: variant of primary effusion lymphoma?
Authors: Lambe Jennifer S. , Oble Darryl A. , Nandula Subhadra V. , Sevilla Deborah W. , Colovai Adriana I. , Mansukhani Mahesh , Chari Ajai , Murty Vundavalli V. , Alobeid Bachir , Bhagat Govind 
Published: March 2009

AbstractPrimary effusion lymphoma (PEL) is a rare type of B‐cell non‐Hodgkin lymphoma (NHL), which predominantly occurs in HIV‐infected individuals, and is pathogenetically linked with Kaposi sarcoma (KS)‐associated herpes virus/human herpes virus‐8 (KSHV/HHV‐8) infection with or without evidence of Epstein–Barr virus (EBV) co‐infection. Although uncommon, PELs have been reported in immunocompetent patients and recipients of solid organ allografts. Rare cases of KSHV− EBV+ post‐transplant effusion lymphomas resembling PEL have also been described, as have KSHV− EBV− effusion lymphomas, the latter including those arising in individuals with chronic liver disease. We report a unique KSHV− EBV− post‐transplant effusion lymphoma associated with serum paraproteins, occurring in an HIV− individual, which had cytologic features and phenotype similar to PEL, and displayed a complex karyotype including isochromosome 12p and translocation t(8;22), resulting in rearrangement of c‐MYC. Copyright © 2009 John Wiley & Sons, Ltd.
Downloads and citations in Intelligent Systems in Accounting, Finance and Management
Authors: O'Leary Daniel E. 
Published: March 2009

AbstractThis paper summarizes the papers downloaded most from the years 2000–2002 and traces the number of citations from Google Scholar (beta) for those papers at the beginning of 2008. It is found that the number of downloads and citations are highly correlated, suggesting that downloads is a leading indicator of citations, even years into the future. In addition, this paper assesses which of the papers from the journal have been cited most over the history of the journal, using both ISI–Social Science Citation Index and Google Scholar. It is found that the numbers of citations using both approaches are highly correlated. Copyright © 2009 John Wiley & Sons, Inc.