bluuebunny/crossref_metadata_2025_split huggingface dataset:
Can the digital economy imporve the efficiency of synergistic management of pollution and carbon emissions?Evidence from cities in China
Authors: Yan Jian Jun , Deng Nan 
Published: June 2024

Abstract

Achieving synergies in reducing pollution and carbon emissions has emerged as a crucial strategic goal in the new development phase. To investigates whether digital economy(DE), as a novel dynamic energy in this stage, can effectively contribute to the reduction of pollutants and carbon emissions. Accordingly, this study focuses on 265 cities in China spanning from 2011 to 2019. The study introduces the concept of the efficiency of synergistic management of pollutants and carbon emissions (SEPC). It utilizes an econometric model to empirically examine the pollution and carbon reduction effects of digital ecomomy, along with its implementation path. The findings reveal the following: (1) The impact of DE on SEPC exhibits a noticeable non-linear pattern, resembling a “U” shape. Moreover, significant spatial spillovers resulting from this nonlinearity are observed. (2) The mechanism analysis indicates that DE influences SEPC by affecting the energy framework, promoting green technology innovation, shaping industrial composition, and influencing public concern for the environment. (3) Heterogeneity analysis suggests that the influence of DE on SEPC is more pronounced in innovative cities, smart cities, and cities with higher levels of government intervention. This study validates the pivotal role of DE in advancing synergies for pollution and carbon reduction. Furthermore, it serves as a valuable reference for achieving dual carbon objectives.
Bee venom reduces early inflammation and oxidative stress associated with lipopolysaccharide-induced alpha-synuclein in the substantia nigra-striatum axis
Authors: Lomeli-Lepe Alma Karen , Castañeda-Cabral José Luis , Ureña-Guerrero Mónica E. , Cabrera Graciela Gudiño , López-Pérez Silvia Josefina 
Published: June 2024

Abstract

Neuroinflammation and oxidative stress are important features in the pathogenesis and development of synucleinopathies, the glial activation and upregulation of pro-inflammatory and oxidative mediators induce alpha-synuclein (α-syn) accumulation. Recent studies have shown that bee venom (BV) has beneficial effects on symptoms of these neurodegenerative diseases. BV is known to exert anti-inflammatory and anti-oxidative effects. Here, we investigated the effects of BV over the different inflammatory and oxidative markers, and in the expression of α-syn and tyrosine hydroxylase (TH) in a lipopolysaccharide (LPS)-induced rat model of synucleinopathies. We examined whether BV (1.5 mg/kg by acupoint injection ST36 six times every 48 hours) could change the α-syn and TH expression measured by western blotting, also, observed the activation of microglia and astrocytes by immunofluorescence, quantify the proinflammatory cytokines levels (TNF-α and IL-1β) by ELISA, and estimated the lipid peroxidation and the activity of superoxide dismutase (SOD) and catalase (CAT) by colorimetric kits in LPS-treated rats (2.5 µg by a single dose intranigral injection) in substantia nigra (SN) and striatum (STR) brain areas. In the LPS-injected rat brain, BV treatment reduced α-syn levels and increased the TH levels. In addition, we observed lower microglia and astrocyte activation in SN and STR. Furthermore, BV decreases IL-1β and lipid peroxidation and increases the CAT activity in the STR. These results indicate that BV can restore the α-syn and TH levels possibly by the inhibition of LPS-induced neuroinflammation and oxidation, also, these results suggest that BV could be a promising treatment option for synucleinopathies.
The spread of the invasive mosquito Aedes albopictus (Diptera: Culicidae) in Portugal: a first genetic analysis
Authors: Zé-Zé Líbia , Freitas Inês Campos , Silva Manuel , Soares Patrícia , Alves Maria João , Osório Hugo Costa 
Published: June 2024

Abstract

Background
Aedes albopictus, commonly known as the Asian tiger mosquito, has become one of the most invasive mosquito species, introduced and established in various tropical and temperate regions worldwide over the last five decades. First reported in Europe in 1979 in Albania and later in Italy in 1990, the species is now established in 13 EU/EEA countries and 337 regions (2023). In Portugal, Ae. albopictus was first detected in the Algarve and Penafiel regions in 2017, followed by Alentejo in 2022 and Lisbon in 2023. This mosquito species poses a significant public health risk as a vector for numerous pathogenic viruses, including dengue, Zika and chikungunya.
Methods
Aedes albopictus collected in Lisbon in 2023, were analyzed using COX gene sequencing to understand their genetic relationships.
Results
Our data indicate that the Ae. albopictus mosquito populations detected in three different locations in Lisbon in 2023 correspond to recent, but distinct introduction events.
Conclusions
Although there has been no local transmission of Aedes-transmitted viruses in mainland Portugal to date, the spread of the mosquito and increased international travel increase the risk of Aedes-borne disease outbreaks. The ongoing spread of Ae. albopictus in the country and the confirmed multiple introductions in new locations raise awareness of the need to monitor mosquito vectors in order to control and prevent autochthonous Aedes-borne disease outbreaks.
Sex-mediated gene flow of grayfoot chacma baboons (Papio ursinus griseipes) in a highly seasonal habitat in Gorongosa National Park
Authors: Silva Maria J Ferreira da , Tralma Paula , Colmonero-Costeira Ivo , Mota Mariana , Farassi Rassina , Hammond Philippa , Lewis-Bevan Lynn , Bamford Marion K. , Biro Dora , Lüdecke Tina , Mathe Jacinto , Bobe René , Capelli Cristian , Carvalho Susana , Martinez Felipe I. 
Published: June 2024

Abstract

Investigating primates’ behavioral variation at the inter-population level is important for the understanding of the evolutionary processes leading to species-specific patterns. The study of behavioral diversity among populations also contributes to improving’ primate conservation efforts. Dispersal patterns tend to be similar among close phylogenetic lineages but may vary in response to individual-based responses. Here, we investigate dispersal patterns of chacma baboons (Papio ursinus griseipes) living in Gorongosa National Park (GNP) and the Catapu Forest Reserve (CFR) in central Mozambique. The park consists of a mosaic landscape, located in a seasonally variable area. GNP was the epicenter of a major war, which severely reduced most apex predators resulting in limited mammalian predation on baboons and a steep increase in number of groups and/or group’s fission. We used a genetic dataset of 121 non-invasive DNA samples analyzed for uni- and bi-parentally inherited markers aiming to characterize the spatial distribution of genetic variation and investigate the extent and direction of sex-mediated gene flow at different time scales. We found high levels of genetic diversity as estimated using autosomal microsatellite loci data and no evidence for a significant contraction of the population size in the last generations. A very distinct mitochondrial DNA haplotype was sampled in CFR. We found evidence for historical and instantaneous male-biased dispersal and female philopatry, estimated among localities and at short distances in GNP, respectively. Our study highlights the strong conservation of sex-biased dispersal patterns and philopatry in chacma baboons and suggests that dispersal behaviors in chacma baboons are resilient to environmental changes and seasonality.
EMPREENDEDORISMO SOCIAL
Authors: Lamberti Tatiane Balbinot , Ávila Lucas Veiga , Rosa Carmen Brum 
Published: June 2024

Em vista do crescimento de pesquisas sobre empreendedorismo social e assuntos relacionados, notou-se a necessidade de analisar as principais características, tendências e desafios sobre o estudo do empreendedorismo social dos últimos anos, a fim de sintetizar os principais avanços e trilhar um caminho para pesquisas futuras. Para isso, realizou-se uma Revisão Sistemática da Literatura, utilizando a base de dados Web of Science. O período estabelecido foi de 01/01/2018 a 30/06/2023 e, para a coleta de dados, foram utilizados o conjunto de palavras-chaves e operadores booleanos: “inovação social” & “negócios” & “empreendedorismo social”. Foi utilizado filtro para selecionar “Artigos” e “Artigos de Revisão”. Foram encontrados 321 artigos que, após a aplicação da pergunta filtro, somaram 186 artigos. Para auxiliar na organização e análise dos estudos, foram utilizados os softwares Mendeley e VOSviewer Como resultado, observou-se que os países desenvolvidos tiveram predominância na quantidade de publicações, com destaque para os Estados Unidos da América e a Inglaterra. Entretanto, quando se analisa a quantidade de publicações por instituição e por autores, países em desenvolvimento lideram esse ranking, representados por instituições como a Tecnológico de Monterrey (México) e a Thapar Institute of Engineering Technology (Índia), sendo esta a instituição com maior número de citações. A principal área de pesquisa é a de “Economia de Negócios”, contida em 62,9% dos estudos. Novas vertentes de pesquisa são identificadas nos temas de “Relacionamentos em Rede” e “Capacidade de Escala das Inovações”, apresentando as principais lacunas atuais.
Non-surgical Synergistic Interventions for the Treatment of Skin Cancer
Authors: Abobaker Magdi , Govender Mershen , Choonara Yahya E. 
Published: June 2024

Abstract:
Skin cancer is broadly classified into two categories i.e., non-melanoma skin cancer (NMSC) and
malignant melanoma (MM), with MM having a greater fatality rate than NMSC. A large number of treatment
strategies currently exist for these skin cancer types, ranging from monotherapies to complex multifaceted synergistic
interventions including dual therapies, trimodality therapy, and multicomponent combinations therapy.
These combinatorial cancer treatments have delivered more favorable results when compared with monotherapies,
and although combination treatments increase the cost of treatment, these regimens have lower side effect
profiles, decreased resistance, high efficacy and an improved long-term response. Synergistic combination
treatments for skin cancer are often complex, wide-ranging and encompass diverse platforms with various
mechanisms of action. An understanding of the physiological potential, as well the efficacy of such treatments,
is therefore vital to ensure patients receive the best possible treatment. This review therefore focuses on the
current advancements and existing non-surgical combinative drug delivery methods utilized for treating skin
cancer. It encompasses the diverse pharmaceutical delivery systems, clinical outcomes, and oncology strategies
employed and aims to highlight the role of non-surgical combination therapies in enhancing patient compliance,
reducing treatment durations, and improving overall survival rates while addressing relapses and metastasis.
The promising outlook of the research being conducted in this field has also been provided, as well as
the barriers to the effective treatment of this complex condition.
Novel Therapies for ANCA-associated Vasculitis: Apilimod Ameliorated Endothelial Cells Injury through TLR4/NF-κB Pathway and NLRP3 Inflammasome
Authors: Liu Siyang , Cao Chenlin , Wang Yiru , Hu Liu , Liu Qingquan 
Published: June 2024

Background:
Antineutrophil cytoplasmic antibody-associated vasculitis (AAV) is a rapidly progressive
form of glomerulonephritis for which effective therapeutic drugs are currently lacking, and its underlying
mechanism remains unclear.

Aims:
This study aimed to investigate new treatment options for AAV through a combination of bioinformatics
analysis and cell molecular experiments.

Methods:
The research utilized integrated bioinformatics analysis to identify genes with differential expression,
conduct enrichment analysis, and pinpoint hub genes associated with AAV. Potential therapeutic compounds
for AAV were identified using Connectivity Map and molecular docking techniques. In vitro experiments
were then carried out to examine the impact and mechanism of apilimod on endothelial cell injury induced
by MPO-ANCA-positive IgG.

Results:
The findings revealed a set of 374 common genes from differentially expressed genes and key modules
of WGCNA, which were notably enriched in immune and inflammatory response processes. A proteinprotein
interaction network was established, leading to the identification of 10 hub genes, including TYROBP,
PTPRC, ITGAM, KIF20A, CD86, CCL20, GAD1, LILRB2, CD8A, and COL5A2. Analysis from Connectivity
Map and molecular docking suggested that apilimod could serve as a potential therapeutic cytokine inhibitor
for ANCA-GN based on the hub genes. In vitro experiments demonstrated that apilimod could mitigate
tight junction disruption, endothelial cell permeability, LDH release, and endothelial activation induced by
MPO-ANCA-positive IgG. Additionally, apilimod treatment led to a significant reduction in the expression of
proteins involved in the TLR4/NF-κB and NLRP3 inflammasome-mediated pyroptosis pathways.

Conclusion:
This study sheds light on the potential pathogenesis of AAV and highlights the protective role of
apilimod in mitigating MPO-ANCA-IgG-induced vascular endothelial cell injury by modulating the TLR4/
NF-kB and NLRP3 inflammasome-mediated pyroptosis pathway. These findings suggest that apilimod may
hold promise as a treatment for AAV and warrant further investigation.
Exploring the Therapeutic Potential of Triptonide in Salivary Adenoid Cystic Carcinoma: A Comprehensive Approach Involving Network Pharmacology and Experimental Validation
Authors: Geng Shikai , Chen Li , Lin Wanzun , Wan Fangzhu , Le Ziyu , Hu Wei , Chen Huaiyuan , Liu Xingyu , Huang Qingting , Zhang Haojiong , Lu Jiade J. , Kong Lin 
Published: June 2024

Background:
Salivary Adenoid Cystic Carcinoma (ACC) is characterized by a highly invasive and
slow-growing pattern, and its etiology remains unidentified. Triptonide (TN) has demonstrated efficacy as a
pharmacotherapeutic agent against ACC. Nonetheless, the specific targets and mechanism of molecular action
underlying the effectiveness of TN in treating ACC have not been elucidated.

Objectives:
By integrating network pharmacology within laboratory experiments, this research delves into the
prospective targets and molecular mechanisms associated with the application of TN in treating ACC.

Methods:
Initially, pertinent targets associated with TN against ACC were acquired from public databases.
Subsequently, a combination of network pharmacology and bioinformatics analysis was utilized to screen the
top 10 hub targets and key signal pathways of TN-treating ACC. Finally, in vitro experiments involving various
molecular assays were conducted to evaluate the biological phenotypes of cells following TN treatment,
encompassing assessments of apoptosis levels, plate migration, and other parameters, thereby validating pivotal
genes and pathways.

Results:
A total of 23 pertinent targets for TN in relation to ACC were identified, with the top 10 hub genes being
MAPK8, PTGS2, RELA, MAPK14, NR3C1, HDAC1, PPARG, NFKBIA, AR, and PGR. There was a significant
correlation between the TNF signaling pathway and the treatment of ACC with TN. In vitro experiments
demonstrated that TN treatment elevated RELA phosphorylation while concurrently reducing MAPK14
phosphorylation and inducing G2/M arrest. TN exhibited the ability to enhance the apoptosis rate through increased
caspase-3 activity, elevated levels of Reactive Oxygen Species (ROS), mitochondrial dysfunction, and
inhibition of cell migration.

Conclusion:
There is a potential therapeutic role for TN in the treatment of ACC through the activation of the
TNF signaling pathway. Among the identified candidates, MAPK8, HDAC1, PTGS2, RELA, NR3C1,
PPARG, NFKBIA, AR, and PGR emerge as the most pertinent therapeutic targets for TN in the context of
ACC treatment.
Study on the Mechanism of the Combination of Methotrexate and Leflunomide in the Treatment of Rheumatoid Arthritis Based on Network
Pharmacology, Molecular Docking, and in vitro Experimental Verification
Authors: Shi Jinyang , Cui Xinhua , Wang Yang , Song Yuli , Tang Xudong , Fan Junwen , Xu Hongyue , Zhu Mingmei , Yu Wanlu , Yu Lu 
Published: June 2024

Background:
To date, disease-modifying antirheumatic drugs (DMARDs) are widely
used as the primary first-line treatment option for patients with rheumatoid arthritis (RA), and
the curative effect of methotrexate (MTX) and leflunomide (LEF; MTX + LEF) is greater than
that of single-agent MTX therapy, but the synergistic mechanism of MTX + LEF is unclear.

Methods:
First, we explored the mechanism of action of MTX + LEF in RA through network
pharmacology and molecular docking. Venn diagram analysis revealed 97 overlapping gene
targets of MTX + LEF-RA and STRING, along with Cytoscape plug-in MOCDE and cytoHubba;
and GO enrichment analysis revealed that the functions of 97 synergistic targets were related
to 123 molecular functions (MF), 63 cell components (CC), and 1,068 biological processes
(BP). The Cytoscape plug-in ClueGO demonstrated that these targets were enriched in KEGG
pathways of 52 terms, whereas 9 pivotal genes were mainly involved in the signaling pathways
of estrogen, Ras, Rap1, PI3K-Akt, relaxin, TNF, AMPK, FoxO, prolactin, IL-17, and adherens
junction. Finally, CETSA and DARTS validated the direct binding of MTX or LEF to the selected
target proteins EGFR, PPARG, MMP9, and SRC in RAW264.7 cells.

Results:
We identified 292 MTX targets and 247 LEF targets from 7 databases. Furthermore,
2,814 potential targets of RA were identified by merging 1,925 targets from 7 databases and 999
differentially expressed genes (DEGs) between normal controls and patients with RA extracted
from 5 GEO databases. Nine pivotal genes, ESR1, ALB, CASP3, EGFR, HSP90AA1, SRC,
MMP9, PPARG, and IGF1, were identified. Molecular docking verified that both MTX and
LEF strongly bind to most of the 9 pivotal proteins except ESR1 and IGF1.

Conclusion:
These results contribute to our understanding of the enhancement mechanism of
MTX combined with LEF and provide a targeted basis for the clinical treatment of RA.
Multi-target Compounds against Trypanosomatid Parasites and
Mycobacterium tuberculosis
Authors: Gomes Midiane Correia , Padilha Emanuelly Karla Araújo , Diniz Gustavo Rafael Angelo , Gomes Edilma Correia , da Silva Santos-Júnior Paulo Fernando , Zhan Peng , da Siva-Júnior Edeildo Ferreira 
Published: June 2024

:
Multi-target drug treatment has become popular as a substitute for traditional monotherapy.
Monotherapy can lead to resistance and side effects. Multi-target drug discovery is gaining
importance as data on bioactivity becomes more abundant. The design of multi-target drugs is expected
to be an important development in the pharmaceutical industry in the near future. This review
presents multi-target compounds against trypanosomatid parasites (Trypanosoma cruzi, T.
brucei, and Leishmania sp.) and tuberculosis (Mycobacterium tuberculosis), which mainly affect
populations in socioeconomically unfavorable conditions. The article analyzes the studies, including
their chemical structures, viral strains, and molecular docking studies, when available. The objective
of this review is to establish a foundation for designing new multi-target inhibitors for these
diseases.
A Low-cost and Rapid Method for Determination of Five Flavonoids in Herbal Medicines with Rutin by HPLC-UV at Equal Absorption Wavelength
Authors: Qian Zhengming , He Zhuobin , Lian Dongyin , Huang Qi , Tan Guoying , Xie Juying 
Published: June 2024

Background:
Flavonoid is a type of active constituent in herbs and always used as the
quality control markers of herbal medicines. Owing to the extensive diversity of flavonoids, numerous
reference compounds are necessitated for the analysis of flavonoids, and some are usually very
expensive, which engenders challenges in the analysis of flavonoids in herbal medicines. Consequently,
the development of a simple, rapid, and reference compounds saving method is important
for the determination of flavonoids in herbal medicines.

Objective:
In order to develop a high-performance liquid chromatography (HPLC) method for the
determination of 5 flavonoids (mangiferin, hesperidin, baicalin, buddleoside, and rutin) in five herbal
medicines (Anemarrhenae rhizome, Sophorae flos, Citri reticulatae pericarpium, Scutellariae radix,
and Chrysanthemi indici flos) with rutin.

Methods:
Five herbal medicine samples were prepared according to the Chinese Pharmacopoeia
which includes ultrasound and reflux methods. The separation of the sample was performed on a
PoroShell 120 EC-C18 (4.6 mm×100 mm, 2.7 μm) by gradient elution with 0.1% formic acid and
acetonitrile at a flow rate of 1.0 mL/min. The wavelengths were set as follows: Anemarrhenae rhizome
(363 nm), Sophorae flos (256 nm), Citri reticulatae pericarpium (236 nm), Scutellariae radix
(263 nm), Chrysanthemi indici flos (354 nm).

Results:
The method validation showed that the established HPLC method was accurate and stable
for quantitative analysis of flavonoids in five herbal medicines. The comparative analysis revealed
that the determination results of the current HPLC method and Chinese Pharmacopoeia method are
consistent, exhibiting less than 1% relative error. Remarkably, the developed HPLC method needs
one cheapest reference compound (rutin) and costs 8 min for sample HPLC analysis.

Conclusion:
The developed HPLC method for quantitative analysis of five flavonoids in five herbal
medicines is simple, rapid, and reference compound saving, which provides a good alternative method
for quality control of flavonoids in herbal medicines.
A Comprehensive Review on History, Sources, Biosynthesis, Chemical Synthesis and Applications of Stilbenes
Authors: Shafiq Nusrat , Jannat Asna , Rashid Maryam , Parveen Shagufta , Noor Nadia 
Published: June 2024

Abstract:
This review is about a class of plant polyphenols known as Stilbenes. Resveratrol – the
first stilbene was extracted from White Hellebore in 1940, since then 400 plus stilbene derivatives
have been discovered. The core purpose of this paper is to summarize the history, extraction
sources, biosynthesis and chemical synthesis and applications of stilbenes. Apart from biosynthesis,
its novel derivatives are being synthesized in laboratories. This class of compounds has extensive
clinical (including antioxidant, anticancer, anti-inflammatory activities etc.) and industrial applications
such as optical dyes, laser dyes, scintillators, etc.
Deciphering the Cardiovascular Conundrum of COVID-19: An In-depth
Review
Authors: Yadav Shiv Kumar , Chand Sharad 
Published: June 2024

Abstract:
The coronavirus disease pandemic raised a notable deal of discussion among scientists
and the public, in addition to necessitating the adaptation of healthcare staff for nearly two years.
COVID-19 caused various respiratory symptoms and lung damage. In addition, it also played a major role in insulting the circulatory system. The frequency of heart-lung involvement with its inverse
relationship with prognosis is highlighted in the review. Direct cardiac involvement (COVID-19
myocarditis) was followed by subsequent cardiac involvement (myocardial damage, myocardial
infarction, and arrhythmias). The coronavirus disease vaccination's continual effects, cardiovascular
problems, and the way the virus aggravated preexisting cardiovascular illness were ultimately studied in this review.