bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perceived family health in persons with prostate cancer and their family members
Authors: Harju Eeva , Rantanen Anja , Tarkka Marja‐Terttu , Åstedt‐Kurki Päivi 
Published: August 2011

Aims and objectives.  To describe perceived family health in persons with prostate cancer and their family members. An additional purpose was to describe the associations between the background variables of persons with prostate cancer and their family members and perceived family health.Background.  Prostate cancer in one family member affects the health of the whole family. It is important to study perceived family health in families of persons with prostate cancer and associated factors.Design.  A cross‐sectional study.Method.  Data were collected using the Family Functioning, Health and Social Support (FAFHES) scale, modified for use in this study. Questionnaires were distributed to 100 persons with prostate cancer treated with surgery or radiation therapy and 100 family members at one Finnish university hospital in April–October 2009. Responses were received from 76 persons and 71 family members, a response rate of 74%.Results.  Persons with prostate cancer and family members rated family health good despite prostate cancer. Family members reported higher levels of illness‐related ill‐being than persons with prostate cancer. Family health was associated with the person’s age, basic education, employment status, number of family member’s visits to the hospitalised patient, first symptom of prostate cancer and previous hospitalisations were associated with. Family health was associated with the family member’s basic and vocational education.Conclusions.  The results show that nursing care should pay attention to family members of persons with prostate cancer and that support to them should be a natural part of a good care of persons with prostate cancer.Relevance to clinical practice.  The quality of prostate cancer care should be improved to provide more individualised and family‐focussed services.
Thrombosis of Tunneled‐Cuffed Hemodialysis Catheters: Treatment With High‐Dose Urokinase Lock Therapy
Authors: Donati Gabriele , Colì Luigi , Cianciolo Giuseppe , La Manna Gaetano , Cuna Vania , Montanari Mara , Gozzetti Francesco , Stefoni Sergio 
Published: August 2011

AbstractThrombosis‐related malfunction of tunneled‐cuffed central venous catheters (TCC) for hemodialysis (HD) currently leads to a high rate of untimely catheter removal. Urokinase (UK) therapy is used for TCC thrombosis/malfunction, but no consensus exists on the adequate dose to obtain thrombolysis. We selected 72 HD patients with TCC and a mean age and HD vintage of 74 years (range 65–87) and 36 months (range 12–61), respectively. All patients received warfarin therapy with a target international normalized ratio (INR) of 1.8–2.5. Coagulative assessment of the patients was obtained by checking the INR, activated partial thromboplastin time, fibrinogen, hemoglobin, and platelets. Sixty‐five thrombotic events were recorded during a 3‐year follow‐up (median 0.3 events/patient/year). The patients selected were randomized into two groups according to a different thrombolytic therapy. Group A comprised 29 thrombotic events in 32 patients who received UK 25 000 IU in both arterial and venous lines of the TCC for each event. UK restored an adequate blood flow rate (BFR) for HD (≥250 mL/min) in 4/29 events (13.7%), whereas addition of 50 000 IU to both arterial and venous lines was required in 25/29 events (86.3%). For the same 25 events in the second HD session, a further 75 000 IU of UK was needed for each TCC lumen. Group B comprised 36 thrombotic events in 40 patients who received 100 000 IU of UK in the arterial and venous lumen of the TCC for each event. An adequate BFR was recovered in all events. In 12/36 events (33.3%), 100 000 IU UK for both lumens were needed in the second HD. In conclusion, group B patients obtained (i) a significantly better TCC patency than group A patients; (ii) a low UK administration in the following HD sessions; and (iii) no bleeding complications.
Impact of Hemoglobin Concentration and Affinity for Oxygen on Tissue Oxygenation: The Case of Hemoglobin‐Based Oxygen Carriers
Authors: Samaja Michele , Terraneo Laura 
Published: August 2011

AbstractIn patients undergoing exchange‐transfusion with hemoglobin (Hb)‐based oxygen (O2) carriers (HBOC), native Hb coexists with newly transfused Hb. The two Hb types share the same arterial and venous PO2, but their affinities for O2 vary. A simple spreadsheet model is described aiming at evaluating the contribution of each Hb type to the overall O2 transport characteristics as a function of the batch Hb concentration and O2 affinity in the HBOC solution, of the fraction of exchange‐transfused blood/HBOC, and of the arterial PO2. This model helps to yield a quantitative estimate of how tissues with high or low O2 extraction respond to the changes cited above. The results show that the higher the exchange‐transfusion ratio, the O2 transport to tissues becomes progressively impaired. However, this effect is more critical at low batch Hb concentration and high O2 affinity of the HBOC, especially for tissues/organs with high O2 extraction, whereas the arterial PO2 does not appear as critical.
Recurrent Ochrobactrum anthropi and Shewanella putrefaciens bloodstream infection complicating hemodialysis
Authors: Shrishrimal Kumarpal 
Published: August 2011

AbstractBloodstream infections (BSIs) are common in hemodialysis, especially when the access is a catheter. These infections are more commonly gram‐positive bacteria or gram‐negative bacilli and on some occasions, fungi. Ochrobactrum anthropi and Shewanella putrefaciens are ubiquitous hydrophilic gram‐negative bacilli. There have been three cases of O. anthropi BSI reported in hemodialysis patients (one from the United States and two from Vienna) and two cases of S. putrefaciens BSI in hemodialysis patients (one from the United States and the other from Japan). There have been few more cases reported of infections with these bacteria in peritoneal dialysis, especially outside the United States. We present a novel case of a patient with both recurrent O. anthropi and S. putrefaciens BSI complicating hemodialysis. There have been no reports in the literature of such a case. We also discuss the microbiology, clinical features, and the challenging aspects of treatment of such infections.
Interproximal cervical lesions caused by incorrect flossing technique
Authors: Salas ML , McClellan AC , MacNeill SR , Satheesh KM , Cobb CM 
Published: August 2011

To cite this article

Int J Dent Hygiene 10, 2012; 83–85  DOI: 10.1111/j.1601‐5037.2011.00524.xSalas ML, McClellan AC, MacNeill SR, Satheesh KM, Cobb CM. Interproximal cervical lesions caused by incorrect flossing technique.Abstract:  This case report describes an interproximal cervical lesion caused by the incorrect use of dental floss. A 58‐year‐old man who was asymptomatic, presented with unusual notch‐like cervical lesions. After clinical and radiographical examinations, it was concluded that the aetiology of these lesions was an incorrect flossing technique. The treatment plan included extraction of maxillary 3rd molars and re‐education of the patient in oral hygiene technique. Conclusion: These lesions are irreversible and often go undiagnosed; therefore, it is important for the clinician to be familiar with the clinical presentation and aetiology.
Echocardiographic and Tissue Doppler Imaging Alterations Associated with Spontaneous Canine Systemic Hypertension
Authors: Misbach C. , Gouni V. , Tissier R. , Trehiou‐Sechi E. , Petit A.M.P. , Carlos Sampedrano C. , Pouchelon J‐L. , Chetboul V. 
Published: August 2011

Background:Feline systemic arterial hypertension (SHT) is associated with a wide spectrum of left ventricular (LV) geometric patterns as well as diastolic, and to a lesser extent, systolic myocardial dysfunction. However, little is known about SHT‐related cardiac changes in dogs.Hypothesis:SHT in dogs is responsible for morphological and functional cardiac alterations.Animals:Thirty dogs with spontaneous untreated SHT and 28 age‐ and body weight‐matched healthy dogs as controls.Methods:Prospective observational study. Conventional echocardiography and 2‐dimensional color tissue Doppler imaging were performed in SHT dogs by trained observers and compared with controls.Results:Forty‐seven percent of SHT dogs (14/30) had diffuse concentric hypertrophy. None had left atrial dilatation and 10/30 (33%) had aortic insufficiency (AoI) associated with proximal aortic dilatation. Longitudinal diastolic left ventricular free wall (LVFW) motion was altered in all SHT dogs at the base (early to late diastolic wave ratio, E/A = 0.5 ± 0.1 versus 1.3 ± 0.3 for controls,P< .0001) and the apex (E/A = 1.6 ± 1.7 versus 3.9 ± 3.1,P< .05). Longitudinal motion of the interventricular septum at the base (E/A = 0.7 ± 0.4 versus 1.1 ± 0.1,P< .01) and radial LVFW motion in the subendocardium (E/A = 0.9 ± 0.5 versus 1.6 ± 0.3,P< .01) were also altered in dogs with SHT. Longitudinal LVFW systolic velocities and gradients were also significantly decreased (P< .05) in SHT dogs.Conclusion and Clinical Importance:As in SHT in cats, SHT in dogs is associated with myocardial dysfunction independently of the presence of myocardial hypertrophy. However, unlike feline SHT, it results in a homogeneous LV geometric pattern with a relatively high prevalence of AoI.
Selective Killing of Bacterial Persisters by a Single Chemical Compound without Affecting Normal Antibiotic-Sensitive Cells
Authors: Kim Jun-Seob , Heo Paul , Yang Tae-Jun , Lee Ki-Sing , Cho Da-Hyeong , Kim Bum Tae , Suh Ji-Hee , Lim Hee-Jong , Shin Dongwoo , Kim Sung-Koo , Kweon Dae-Hyuk 
Published: August 2011

ABSTRACT
We show that 3-[4-(4-methoxyphenyl)piperazin-1-yl]piperidin-4-yl biphenyl-4-carboxylate (C10), screened out of a chemical library, selectively kills bacterial persisters that tolerate antibiotic treatment but does not affect normal antibiotic-sensitive cells. C10 led persisters to antibiotic-induced cell death by causing reversion of persisters to antibiotic-sensitive cells. This work is the first demonstration in which the eradication of bacterial persisters is based on single-chemical supplementation. The chemical should be versatile in elucidating the mechanism of persistence.
A Novel Target-Specific, Salt-Resistant Antimicrobial Peptide against the Cariogenic Pathogen Streptococcus mutans
Authors: Mai Junni , Tian Xiao-Lin , Gallant Jeffrey W. , Merkley Nadine , Biswas Zakia , Syvitski Raymond , Douglas Susan E. , Ling Junqi , Li Yung-Hua 
Published: August 2011

ABSTRACT

In this study, we constructed and evaluated a target-specific, salt-resistant antimicrobial peptide (AMP) that selectively targeted
Streptococcus mutans
, a leading cariogenic pathogen. The rationale for creating such a peptide was based on the addition of a targeting domain of
S. mutans
ComC signaling peptide pheromone (CSP) to a killing domain consisting of a portion of the marine-derived, broad-spectrum AMP pleurocidin to generate a target-specific AMP. Here, we report the results of our assessment of such fusion peptides against
S. mutans
and two closely related species. The results showed that nearly 95% of
S. mutans
cells lost viability following exposure to fusion peptide IMB-2 (5.65 μM) for 15 min. In contrast, only 20% of
S. sanguinis
or
S. gordonii
cells were killed following the same exposure. Similar results were also observed in dual-species mixed cultures of
S. mutans
with
S. sanguinis
or
S. gordonii
. The peptide-guided killing was further confirmed in
S. mutans
biofilms and was shown to be dose dependent. An
S. mutans
mutant defective in the CSP receptor retained 60% survival following exposure to IMB-2, suggesting that the targeted peptide predominantly bound to the CSP receptor to mediate killing in the wild-type strain. Our work confirmed that IMB-2 retained its activity in the presence of physiological or higher salt concentrations. In particular, the fusion peptide showed a synergistic killing effect on
S. mutans
with a preventive dose of NaF. In addition, IMB-2 was relatively stable in the presence of saliva containing 1 mM EDTA and did not cause any hemolysis. We also found that replacement of serine-14 by histidine improved its activity at lower pH. Because of its effectiveness, salt resistance, and minimal toxicity to host cells, this novel target-specific peptide shows promise for future development as an anticaries agent.
Comparison between transcranial Doppler and Coulter counter for detection of lipid micro embolization from mediastinal shed blood reinfusion during cardiac surgery
Authors: Eyjolfsson A , Al-Rashidi F , Dencker M , Scicluna S , Brondén B , Koul B , Bjursten H 
Published: August 2011

Introduction: Lipid micro embolization (LME) from re-transfused shed blood has been postulated to be a potential reason for short- and long-term cognitive dysfunction after cardiac surgery. The purpose of this investigation was to evaluate if transcranial Doppler (TCD) has the capacity to detect LME. Methods: Thirteen patients undergoing cardiopulmonary bypass surgery were investigated. Each patient’s cerebral circulation was monitored with transcranial Doppler during the first two minutes after re-transfusion of shed blood and blood was simultaneously sampled and characterised by a Coulter counter. Results: Strong correlation was found between embolic loads, as measured by transcranial Doppler and Coulter counter (r=0.79, P<0.005). Conclusions: This pilot study shows that non-invasive monitoring by transcranial Doppler could be a potential tool to monitor LME during cardiopulmonary bypass surgery.
Annelid Distal-less/Dlx duplications reveal varied post-duplication fates
Authors: McDougall Carmel , Korchagina Natalia , Tobin Jonathan L , Ferrier David EK 
Published: August 2011

AbstractBackgroundDlx (Distal-less) genes have various developmental roles and are widespread throughout the animal kingdom, usually occurring as single copy genes in non-chordates and as multiple copies in most chordate genomes. While the genomic arrangement and function of these genes is well known in vertebrates and arthropods, information about Dlx genes in other organisms is scarce. We investigate the presence of Dlx genes in several annelid species and examine Dlx gene expression in the polychaetePomatoceros lamarckii.ResultsTwo Dlx genes are present inP. lamarckii, Capitella teletaandHelobdella robusta. TheC. teletaDlx genes are closely linked in an inverted tail-to-tail orientation, reminiscent of the arrangement of vertebrate Dlx pairs, and gene conversion appears to have had a role in their evolution. TheH. robustaDlx genes, however, are not on the same genomic scaffold and display divergent sequences, while, if theP. lamarckiigenes are linked in a tail-to-tail orientation they are a minimum of 41 kilobases apart and show no sign of gene conversion. No expression inP. lamarckiiappendage development has been observed, which conflicts with the supposed conserved role of these genes in animal appendage development. These Dlx duplications do not appear to be annelid-wide, as the polychaetePlatynereis dumeriliilikely possesses only one Dlx gene.ConclusionsOn the basis of the currently accepted annelid phylogeny, we hypothesise that one Dlx duplication occurred in the annelid lineage after the divergence ofP. dumeriliifrom the other lineages and these duplicates then had varied evolutionary fates in different species. We also propose that the ancestral role of Dlx genes is not related to appendage development.
A 5'-region polymorphism modulates promoter activity of the tumor suppressor gene MFSD2A
Authors: Colombo Francesca , Falvella Felicia S , Galvan Antonella , Frullanti Elisa , Kunitoh Hideo , Ushijima Toshikazu , Dragani Tommaso A 
Published: August 2011

Abstract

Background
The MFSD2A gene maps within a linkage disequilibrium block containing the MYCL1-EcoRI polymorphism associated with prognosis and survival in lung cancer patients. Survival discrepancies between Asians and Caucasians point to ethnic differences in allelic frequencies of the functional genetic variations.

Results
Analysis of three single-nucleotide polymorphisms (SNPs) mapping in the MFSD2A 5'-regulatory region using a luciferase reporter system showed that SNP rs12072037, in linkage disequilibrium with the MYCL1-EcoRI polymorphism and polymorphic in Asians but not in Caucasians, modulated transcriptional activity of the MFSD2A promoter in cell lines expressing AHR and ARNT transcription factors, which potentially bind to the SNP site.

Conclusion
SNP rs12072037 modulates MFSD2A promoter activity and thus might affect MFSD2A levels in normal lung and in lung tumors, representing a candidate ethnically specific genetic factor underlying the association between the MYCL1 locus and lung cancer patients' survival.
Lassa hemorrhagic fever in a late term pregnancy from northern sierra leone with a positive maternal outcome: case report
Authors: Branco Luis M , Boisen Matt L , Andersen Kristian G , Grove Jessica N , Moses Lina M , Muncy Ivana J , Henderson Lee A , Schieffellin John S , Robinson James E , Bangura James J , Grant Donald S , Raabe Vanessa N , Fonnie Mbalu , Sabeti Pardis C , Garry Robert F 
Published: August 2011

Abstract
Lassa fever (LF) is a devastating viral disease prevalent in West Africa. Efforts to take on this public health crisis have been hindered by lack of infrastructure and rapid field deployable diagnosis in areas where the disease is prevalent. Recent capacity building at the Kenema Government Hospital Lassa Fever Ward (KGH LFW) in Sierra Leone has lead to a major turning point in the diagnosis, treatment and study of LF. Herein we present the first comprehensive rapid diagnosis and real time characterization of an acute hemorrhagic LF case at KGH LFW. This case report focuses on a third trimester pregnant Sierra Leonean woman from the historically non-endemic Northern district of Tonkolili who survived the illness despite fetal demise.
Employed in this study were newly developed recombinant LASV Antigen Rapid Test cassettes and dipstick lateral flow immunoassays (LFI) that enabled the diagnosis of LF within twenty minutes of sample collection. Deregulation of overall homeostasis, significant hepatic and renal system involvement, and immunity profiles were extensively characterized during the course of hospitalization. Rapid diagnosis, prompt treatment with a full course of intravenous (IV) ribavirin, IV fluids management, and real time monitoring of clinical parameters resulted in a positive maternal outcome despite admission to the LFW seven days post onset of symptoms, fetal demise, and a natural still birth delivery. These studies solidify the growing rapid diagnostic, treatment, and surveillance capabilities at the KGH LF Laboratory, and the potential to significantly improve the current high mortality rate caused by LF. As a result of the growing capacity, we were also able to isolate Lassa virus (LASV) RNA from the patient and perform Sanger sequencing where we found significant genetic divergence from commonly circulating Sierra Leonean strains, showing potential for the discovery of a newly emerged LASV strain with expanded geographic distribution. Furthermore, recent emergence of LF cases in Northern Sierra Leone highlights the need for superior diagnostics to aid in the monitoring of LASV strain divergence with potentially increased geographic expansion.
An integrative approach to inferring biologically meaningful gene modules
Authors: Cho Ji-Hoon , Wang Kai , Galas David J 
Published: August 2011

Abstract

Background
The ability to construct biologically meaningful gene networks and modules is critical for contemporary systems biology. Though recent studies have demonstrated the power of using gene modules to shed light on the functioning of complex biological systems, most modules in these networks have shown little association with meaningful biological function. We have devised a method which directly incorporates gene ontology (GO) annotation in construction of gene modules in order to gain better functional association.

Results
We have devised a method, Semantic Similarity-Integrated approach for Modularization (SSIM) that integrates various gene-gene pairwise similarity values, including information obtained from gene expression, protein-protein interactions and GO annotations, in the construction of modules using affinity propagation clustering. We demonstrated the performance of the proposed method using data from two complex biological responses: 1. the osmotic shock response in Saccharomyces cerevisiae, and 2. the prion-induced pathogenic mouse model. In comparison with two previously reported algorithms, modules identified by SSIM showed significantly stronger association with biological functions.

Conclusions
The incorporation of semantic similarity based on GO annotation with gene expression and protein-protein interaction data can greatly enhance the functional relevance of inferred gene modules. In addition, the SSIM approach can also reveal the hierarchical structure of gene modules to gain a broader functional view of the biological system. Hence, the proposed method can facilitate comprehensive and in-depth analysis of high throughput experimental data at the gene network level.
Downregulation of CDKN2A and suppression of cyclin D1 gene expressions in malignant gliomas
Authors: Liu Weidong , Lv Guohua , Li Yawei , li Lei , Wang Bing 
Published: August 2011

Abstract

Background
Malignant gliomas are the most common in central nervous system cancer. Genome-wide association study identifies that CDKN2A was a susceptibility loci for glioma. The CDKN2A/cyclin-dependent kinase 4, 6/Retinoblastoma protein (Rb) pathway is thought to play a crucial role in malignant gliomas pathogenesis. We have investigated the expression of CDKN2A for potential correlations with malignant gliomas grade and potential role of CDKN2A on malignant gliomas pathogenesis.

Methods
Tumour tissue samples from 61 patients suffering from malignant gliomas were investigated. The expression levels of CDKN2A were detected using immunohistochemical staining and western blot. Overexpression and knockdown of CDKN2A were performed in human glioma cell lines. Subsequently, colony formation, growth curves and CDKN2A-Cyclin-Rb pathway were analyzed.

Results
Here we show that a lower expression of CDKN2A and a higher expression of cyclin D1 in the patients with high-grade malignant gliomas than low-grade gliomas, respectively. Moreover, overexpression of CDKN2A inhibits growth of glioma cell lines by suppression of cyclin D1 gene expression.

Conclusions
Our study suggests that CDKN2A as a malignant gliomas suppressor gene, appears to be useful for predicting behaviour of high-grade malignant gliomas. CDKN2A-Cyclin-Rb pathway plays a key role on malignant gliomas formation and that therapeutic targeting of this pathway may be useful in malignant gliomas treatment.