bluuebunny/crossref_metadata_2025_split huggingface dataset:
Endothelial cells incubated with platelet‐rich plasma express PDGF‐B and ICAM‐1 and induce bone marrow stromal cell migration
Authors: Cenni Elisabetta , Ciapetti Gabriela , Granchi Donatella , Fotia Caterina , Perut Francesca , Giunti Armando , Baldini Nicola 
Published: April 2009

AbstractPlatelet‐rich plasma (PRP) is used to accelerate bone repair through the growth factors released by platelets. The purpose of this study was to evaluate if PRP induce human umbilical vein endothelial cells (HUVEC) to express mRNA for osteogenic growth factors and stimulate the migration of bone marrow stromal cell (BMSC). The effects of PRP were compared to those induced by vascular endothelial growth factor‐A (VEGF‐A) or, as a negative control, by platelet poor plasma (PPP). After incubation with PRP, but not with PPP, HUVEC showed an increased expression of mRNA for platelet derived growth factor‐B (PDGF‐B), and this effect was not inhibited by an anti‐VEGF‐A antibody. The migration of BMSC was more stimulated by HUVEC incubated with PRP than by HUVEC incubated with low serum medium or PPP. Besides, PRP increased the expression of intercellular adhesion molecule‐1 (ICAM‐1) and osteoprotegerin, but did not affect the expression either of the receptor activator for nuclear factor κB ligand (RANKL) or of RANK. These findings support the hypothesis that PRP contribute to bone repair by favoring the pro‐osteogenic function of endothelial cells, including the recruitment of osteoblast precursors and the expression of adhesion molecules for monocyte/macrophages, while inhibiting their pro‐osteolytic properties. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27:1493–1498, 2009
Characterization of pottery from the Republic of Macedonia I: Raman analyses of Byzantine glazed pottery excavated from Prilep and Skopje (12th–14th century)
Authors: Tanevska Vinka , Colomban Philippe , Minčeva‐Šukarova Biljana , Grupče Orhideja 
Published: April 2009

AbstractIn order to gain some understanding of and to characterize the materials used in Byzantine glazed ceramic finds in the Republic of Macedonia, as well as to obtain information on their manufacturing technology, micro‐Raman spectra of 15 representative glazed shards, all dated from 12th to 14th century, were recorded. The elemental composition of the body and glaze of selected shards was obtained by scanning electron microscopy‐energy dispersive X‐ray spectrometry (SEM‐EDXS). At least 17 different minerals in the ceramic body were identified from the Raman spectra, among which were a variety of feldspars (microcline, albite and sanidine) as well as andradite, apatite and forsterite. According to the identified minerals, locations of the used raw materials in the vicinity of the archaeological sites are proposed. There is a good correlation between the polymerization index derived from the Raman spectra and the lead content obtained from the SEM‐EDXS analyses, therefore the firing temperature of the analyzed glazes could be assessed. The SEM‐EDXS analyses showed the presence of different coloring agents (Cu, Co, Cr, Sb) as well as opacifying (Sb) and fluxing (Pb) agents in the glaze matrix. The content of P2O5 in the glaze of one of the shards is also discussed. Copyright © 2009 John Wiley & Sons, Ltd.
Systemic inflammatory response predicts postoperative outcome in patients with liver metastases from colorectal cancer
Authors: Ishizuka Mitsuru , Kita Junji , Shimoda Mitsugi , Rokkaku Kyu , Kato Masato , Sawada Tokihiko , Kubota Keiichi 
Published: April 2009

AbstractBackgroundFew studies have investigated the Glasgow Prognostic Score (GPS) in patients with liver metastases from colorectal cancer (LM‐CRC).MethodsThe GPS was calculated as follows: patients with both an elevated level of CRP (>10 mg/L) and hypoalbuminemia (Alb <35 g/L) were allocated a score of 2, and patients showing one or neither of these blood chemistry abnormalities were allocated a score of 1 or 0, respectively.ResultsNinety‐three patients were evaluated retrospectively. Kaplan–Meier analysis and log rank test revealed that a higher GPS predicted a higher postoperative death (P < 0.0001). Univariate analysis revealed that sex, number of hepatectomy, number of tumors, synchronous lung metastasis and CRP were associated with postoperative death. Multivariate analysis revealed that number of hepatectomy (odds ratio, 3.193; 95% CI, 1.093–9.330; P = 0.0338), number of tumors (odds ratio, 2.946; 95% CI, 1.094–7.931; P = 0.0325), synchronous lung metastasis (odds ratio, 3.424; 95% CI, 1.055–11.11; P = 0.0404) and CRP (odds ratio, 4.509; 95% CI, 1.313–15.49; P = 0.0167) were associated with postoperative death.ConclusionsGPS is able to classify patients with LM‐CRC into three independent groups. Among the selected factors, CRP is considered an important and high sensitive predictor of postoperative death in such patients. J. Surg. Oncol. 2009;100:38–42. © 2009 Wiley‐Liss, Inc.
Surgery does not adversely affect survival in primary gastrointestinal lymphoma
Authors: Cheung Michael C. , Housri Nadine , Ogilvie Michael P. , Sola Juan E. , Koniaris Leonidas G. 
Published: April 2009

AbstractObjectiveTo evaluate the impact of surgery on gastrointestinal lymphoma.MethodsThe Surveillance, Epidemiology, and End Results (SEER) database was queried from 1973 to 2005.ResultsA total of 17,222 cases of PGIL were identified. The overall incidence of PGIL was approximately 1.505 cases per 100,000. A significantly increasing incidence for PGIL was observed (APC = +4.67, P < 0.05). In the cases for which treatment data was available, resection occurred in roughly half of the patients. In univariate analysis, surgical extirpation did not improve survival (47 months vs. 76 months, P < 0.001), while radiation treatment improved median survival (77 months vs. 59 months, P < 0.001). Multivariate analysis revealed increasing age and male gender as independent predictors of decreased overall survival. Tumor location also was a significant predictor of outcome. Large B‐cell lymphoma type PGIL had a poorer prognosis than marginal zone B‐cell lymphoma. By multivariate analysis, surgery was not found to increase the risk of death (HR = 0.99).ConclusionsNo associated survival benefit for surgery in the treatment in gastrointestinal lymphoma was observed. Determination of lymphoma should preclude surgical resection. Nonetheless, inadvertent extirpative surgery or in association with perforation does not appear to increase mortality. J. Surg. Oncol. 2009;100:59–64. © 2009 Wiley‐Liss, Inc.
Comparison of C18 silica bonded phases selectivity in micellar liquid chromatography
Authors: Kulikov Artem U. , Galat Marina N. 
Published: April 2009

AbstractThe paper describes a new test designed in micellar LC (MLC) to compare the commercial C18 stationary phase properties. This test provides the total hydrophobicity, hydrophilicity, steric selectivity, hydrogen bonding, and ion‐exchange capacity properties calculation of the ODS stationary phases. Both the test compounds and chromatographic separation conditions choice for column characterization in MLC are detailed. The chromatographic performance of several stationary phases that are used in MLC was evaluated with specific chromatographic test comprising nine test compounds, possessing different physico‐chemical properties, which were injected on different supports with two micellar mobile phases: one at pH 7.0 (0.075 mol/L SDS and 1.5% v/v 1‐pentanol), and other at pH 2.7 (0.075 mol/L SDS and 1.5% v/v 1‐pentanol adjusted to pH by TFA). Fundamental column chromatographic properties were obtained under these conditions and were treated by hierarchical cluster analysis. From the results of cluster analysis, two closely related groups of columns are distinguished, and it was shown that the chosen column characteristic parameters allow characterizing both sorbent and micellar chromatographic system properties. Eleven columns were analyzed by this test, which allows a comparison of columns with the aim of the selection of suitable and analogous column for the analysis with MLC.
Determination of di‐(2‐ethylhexyl)phthalate in environmental samples by liquid chromatography coupled with mass spectrometry
Authors: Garrido Frenich Antonia , de las Nieves Barco Bonilla María , López Martínez Juan Carlos , Martínez Vidal José Luis , Romero‐González Roberto 
Published: April 2009

AbstractDi‐(2‐ethylhexyl)phthalate (DEHP) was determined in environmental samples such as water and soil. DEHP was extracted from water samples using SPE, whereas for soils pressurized liquid extraction was applied as extraction method, using hexane/acetone (1:1, v/v) as extractant solvent. The use of HPLC coupled to MS provides the basis of the selective determination of DEHP in the analyzed samples. The extraction procedures were validated and good results were found. Recoveries were ranged from 86.0 to 99.8% with RSD lower than 18% and LODs were 0.02 mg/kg and 0.03 μg/L for soils and water, respectively. Finally, the optimized methods were applied to the analysis of real samples and DEHP was not found above the LOQ (0.05 mg/kg) in soil samples whereas it was detected in water samples at concentrations ranging between 0.19 to 0.88 μg/L.
Mechanism of interaction of DNA bases with dichloro‐[1‐alkyl‐2‐(naphthylazo)imidazole]palladium(II) complexes: A cytosine case
Authors: Ghosh Pradip Kumar , Saha Sushanta , Mahapatra Ambikesh 
Published: April 2009

AbstractInteraction of cytosine (C) with dichloro‐[1‐alkyl‐2‐(α‐naphthylazo)imidazole]‐palladium(II) [Pd(α‐NaiR)Cl2, 1] and dichloro‐[1‐alkyl‐2‐(β‐naphthylazo)imidazole]‐palladium(II) [Pd(β‐NaiR)Cl2, 2] complexes {where alkyl R = Me (a), Et (b) or Bz (c)} in acetonitrile (MeCN)‐water (50% v/v) medium to yield [{1‐alkyl‐2‐(α‐naphthylazo)‐imidazole}bis(cytosine)]palladium(II)dichloride (3a, 3b, 3c) and [{1‐alkyl‐2‐(β‐naphthylazo)‐imidazole}bis(cytosine)]palladium(II)dichloride (4a, 4b, 4c) was studied. The products were characterized by microanalytical data and spectroscopic techniques (FT‐IR, UV–vis, and NMR). The reaction kinetics show first‐order dependence of the rate on each of the concentration of Pd(II) complex and C. External addition of Cl− ion (LiCl) did not influence this nucleophilic substitution rate process and has proved the cleavage of first PdCl bond is the rate‐determining step. Thermodynamic parameters standard enthalpy of activation (Δ‡Ho) and standard entropy of activation (Δ‡So) were determined from variable temperature kinetic studies. The negative values of Δ‡So indicate that the reaction proceeds through an associative inner sphere mechanism. The magnitude of the second‐order rate constant k2 increases in the following order: Pd(NaiEt)Cl2 (b) < Pd(NaiMe)Cl2 (a) < Pd(NaiBz)Cl2 (c) as well as Pd(α‐NaiR)Cl2 (1) < Pd(β‐NaiR)Cl2 (2), which corroborates with the experimental Δ‡Ho values. © 2009 Wiley Periodicals, Inc. Int J Chem Kinet 41: 463–472, 2009
Mechanism of oxidation of alanine by chloroaurate(III) complexes in acid medium: Kinetics of the rate processes
Authors: Sen Pratik K. , Gani Nasimul , Midya Jayanta K. , Pal Biswajit 
Published: April 2009

AbstractThe kinetics of the oxidation of alanine by chloroaurate(III) complexes in acetate buffer medium has been investigated. The major oxidation product of alanine has been identified as acetaldehyde by 1H NMR spectroscopy. Under the experimental conditions, AuCl and AuCl3(OH)− are the effective oxidizing species of gold(III). The reaction is first order with respect to Au(III) as well as alanine. The effects of H+ and Cl− on the second‐order rate constant k2′ have been analyzed, and accordingly the rate law has been deduced: k2′ = (k1[H+][Cl−] + k3K4K5)/(K4K5 + [H+][Cl−]). Increasing dielectric constant of the medium has an accelerating effect on the reaction rate. Activation parameters associated with the overall reaction have been calculated. A mechanism involving the two effective oxidizing species of gold(III) and zwitterionic species of alanine, consistent with the rate law, has been proposed. © 2009 Wiley Periodicals, Inc. Int J Chem Kinet 41: 473–482, 2009
Gamma knife radiosurgery for vestibular schwannomas: Results of hearing preservation in relation to the cochlear radiation dose
Authors: Timmer Ferdinand C. A. , Hanssens Patrick E. J. , van Haren Anniek E. P. , Mulder Jef J. S. , Cremers Cor W. R. J. , Beynon Andy J. , van Overbeeke Jacobus J. , Graamans Kees 
Published: April 2009

AbstractObjectives/Hypothesis:This study was designed to evaluate hearing preservation after gamma knife radiosurgery (GKRS) and to determine the relation between hearing preservation and cochlear radiation dose in patients with a sporadic vestibular schwannoma (VS).Methods:Prospective study involving patients suffering from VS who received GKRS from June 2003 until November 2007. Pure tone and speech audiometry were conducted before and after GKRS. The thresholds at pure tone audiometry were taken as a measure of hearing. Pure tone average (PTA) was defined as the mean threshold at 0.5 kHz, 1.0 kHz, 2.0 kHz, and 4.0 kHz. Hearing was classified according to the 2003 consensus meeting in Tokyo. Stereotactic surgery was performed using a Leksell 4C Gamma Knife (Elekta, Stockholm, Sweden).Results:A total of 69 patients were included in the study. Mean tumor size was 17 mm. Mean marginal dose at the tumor was 11.0 Gy (range, 9.3 Gy–12.3 Gy), mean maximal dose was 19.7 Gy (range, 16 Gy–25.5 Gy). Mean maximal dose at the cochlea was 10.27 Gy (range, 3.1 Gy–16.1 Gy), and mean minimal dose at the cochlea was 2.6 Gy (range, 0.9 Gy–7.4 Gy). Mean PTA before GKRS was 43 dB (standard deviation [SD] 20 dB), mean PTA after GKRS was 63 dB (SD 30 dB). Mean interval between pre‐GKRS audiometry and GKRS was 8.0 months. Between GKRS and post‐GKRS audiometry, mean interval was 14.2 months. Hearing was considered to be preserved (max +1 class, Tokyo classification) in 52 (75%) of 69 patients. However, only 32 patients had class A, B, or C (serviceable hearing) before GKRS. Within this group, only 13 patients (41%) had a hearing class A, B, or C after GKRS. A significant relation was found between the maximal cochlear dose and the difference in PTA before and after GKRS.Conclusions:Hearing preservation is correlated to the maximal radiation dose at the cochlea. The purpose of developing GKRS techniques was to avoid collateral damage in healthy tissues. This study emphasizes the need for exact radiation planning to reduce the cochlear radiation dose if the hearing is to be preserved. Laryngoscope, 2009
Long‐term great auricular nerve morbidity after sacrifice during parotidectomy
Authors: Ryan William R. , Fee Willard E. 
Published: April 2009

AbstractObjectives/Hypothesis:To clarify the extent and patient perspectives of great auricular nerve (GAN) morbidity and recovery after nerve sacrifice during parotidectomy 4 to 5 years after surgery.Study Design:Prospective series.Methods:Twenty‐two patients who underwent parotidectomy with GAN sacrifice and were previously studied for GAN sensory outcome during the first postoperative year. We performed light touch sensation tests on each patient to develop an ink map representing anesthesia and paresthesia in the GAN sensory territory; patients also completed an outcomes questionnaire.Results:Nineteen (86%) of 22 patients completed follow‐up. One patient completed the questionnaire over the phone. The prevalence and average areas of anesthesia and paresthesia decreased since the first postoperative year according to sensory testing and patient scoring. At 4 to 5 years, 47% (9 of 19) of the patients had anesthesia, 58% (11 of 19) had paresthesia, and 26% (5 of 19) had neither anesthesia nor paresthesia during sensory testing. Patients reported that the GAN dysfunction brought them no to mild inference with their daily activities. At a mean point of 2 years, 70% (14 of 20) patients felt that their sensory symptoms had either completely abated or stabilized.Conclusions:The posterior branch of the GAN should be preserved if it does not compromise tumor resection. If this is not possible, the patient and surgeon should be comforted in that only minor, if any, long‐term disability will ensue. Laryngoscope, 2009
Motor‐related circuit dysfunction in MSA‐P: Usefulness of combined whole‐brain imaging analysis
Authors: Tir Mélissa , Delmaire Christine , le Thuc Vianney , Duhamel Alain , Destée Alain , Pruvo Jean‐Pierre , Defebvre Luc 
Published: April 2009

AbstractThe aim of this study was to evaluate in vivo changes in the brain's macro‐ and microstructure (notably in the motor system) in the parkinsonian variant of multiple system atrophy (MSA‐P) and in Parkinson's disease (PD) and to characterize the cerebral anatomical differences between the two conditions. We used a combination of voxel‐based morphometry (VBM) and whole‐brain, voxel‐based diffusion tensor imaging analysis (VB‐DTI). Forty‐seven right‐handed subjects (14 MSA‐P patients, 19 PD patients, and 14 controls) were evaluated using VBM and VB‐DTI in an analysis of covariance (ANCOVA) with a significance threshold set to P < 0.005. In MSA‐P patients, VBM analysis revealed a lower density of grey matter (GM) in a motor‐related circuit (especially in the left primary motor cortex, PMC), relative to PD patients, and in the left supplementary motor area (SMA), relative to controls). Diffusion tensor imaging analysis revealed lower fractional anisotropy (FA) values in the left PMC and the right cerebellum in MSA‐P patients, compared with controls. Using a volumetric diffusion technique, our study revealed selective tissue degeneration in motor circuits, regardless of the volume loss detected in VBM and in agreement with pathology reports and clinical motor characteristics. Our findings suggest that MSA‐P is characterized by both macro‐ and microstructural changes in the sensorimotor circuit. © 2009 Movement Disorder Society
“Just” in time: Gene theory and the biology of the cell surface
Authors: Sapp Jan 
Published: April 2009

AbstractErnest Everett Just's critique of gene theory, his emphasis on the importance of cell cytoplasm, especially of the cortex in development and heredity, is placed within the context of his times, when the views of embryologists, who emphasized heredity as process, and those of geneticists who held determinist conceptions of the gene, were irreconcilable. Just's emphasis on the cell cortex in heredity and morphogenesis was appreciated by few, but was vindicated by studies of cortical inheritance and the morphogenetic role of pre‐existing cell structure in ciliated protists. His essential criticisms of mechanism remain potent today. Mol. Reprod. Dev. 76: 903–911, 2009. © 2009 Wiley‐Liss, Inc.
Fukutin gene mutations in an Italian patient with early onset muscular dystrophy but no central nervous system involvement
Authors: Saredi Simona , Ruggieri Alessandra , Mottarelli Elisa , Ardissone Anna , Zanotti Simona , Farina Laura , Morandi Lucia , Mora Marina , Moroni Isabella 
Published: April 2009

AbstractHypoglycosylation of α‐dystroglycan characterizes a subgroup of muscular dystrophies of variable severity, including Fukuyama congenital muscular dystrophy. We found fukutin gene mutations in a 4.5‐year‐old Italian patient, with reduced α‐dystroglycan expression, dystrophic features on muscle biopsy, hypotonia since birth, mild myopathy, but no brain involvement. Mutations in the fukutin gene can be associated with much milder phenotypes than classical Fukuyama congenital muscular dystrophy, and, although rare, can occur in non‐Japanese. Muscle Nerve, 2009
Homotopy perturbation method for numerical solutions of KdV‐Burgers' and Lax's seventh‐order KdV equations
Authors: Yildirim Ahmet , Berberler Murat Erşen 
Published: April 2009

AbstractIn this article, we applied homotopy perturbation method to obtain the solution of the Korteweg‐de Vries Burgers (for short, KdVB) and Lax's seventh‐order KdV (for short, LsKdV) equations. The numerical results show that homotopy perturbation method can be readily implemented to this type of nonlinear equations and excellent accuracy. © 2009 Wiley Periodicals, Inc. Numer Methods Partial Differential Eq 2010
Mixed micropapillary–ductal carcinomas of the breast: a genomic and immunohistochemical analysis of morphologically distinct components
Authors: Marchiò Caterina , Iravani Marjan , Natrajan Rachael , Lambros Maryou BK , Geyer Felipe C , Savage Kay , Parry Suzanne , Tamber Narinder , Fenwick Kerry , Mackay Alan , Schmitt Fernando C , Bussolati Gianni , Ellis Ian , Ashworth Alan , Sapino Anna , Reis‐Filho Jorge S 
Published: April 2009

AbstractMicropapillary carcinomas (MPCs) can present as a rare histological special type of breast cancer; however, this histological type is more frequently found admixed with invasive ductal carcinomas of no special type (IDC‐NSTs). We have previously demonstrated that pure MPCs constitute a distinct entity at the morphological and genetic levels. Here, we sought to determine whether mixed MPCs have genomic aberrations similar to those found in pure MPCs, and to investigate whether the distinct morphological components of MPCs harbour different genetic aberrations. Using high‐resolution microarray comparative genomic hybridization (aCGH), we profiled a series of 10 MPCs of mixed histology and 20 IDC‐NSTs matched for grade and oestrogen receptor (ER) status. In addition, we generated tissue microarrays containing a series of 24 pure and 40 mixed MPCs and performed immunohistochemical analysis with ER, progesterone receptor (PR), Ki‐67, HER2, cytokeratin (CK) 5/6, CK14, CK17, EGFR, topoisomerase‐IIα, cyclin D1, caveolin‐1 and E‐cadherin antibodies. In situ hybridization was employed to evaluate the prevalence of HER2, TOP2A, EGFR, CCND1, MYC and FGFR1 gene amplification. Our results demonstrate that mixed MPCs harbour similar patterns of genomic aberrations and phenotype (82.5% luminal and 17.5% HER2) compared to pure MPCs. A comparison between the distinct morphological components of mixed MPCs in a pairwise fashion revealed that both components harbour strikingly similar genomic profiles. When compared to grade‐ and ER‐matched IDC‐NSTs, mixed MPCs significantly more frequently harboured amplification of multiple regions on 8q (adjusted Fisher's p value < 0.05). Furthermore, mixed MPCs displayed higher proliferative rates than grade‐ and ER‐matched IDC‐NSTs. Our results suggest that micropapillary differentiation in breast cancer may identify a subgroup of more aggressive ER‐positive breast carcinomas, even in those featuring a mixed histology, and that mixed MPCs are more closely related to pure MPCs than to IDC‐NSTs. Copyright © 2009 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Aktueller Stand der Gentherapie der schweren Immundefizienz
Authors: Winckler Thomas 
Published: April 2009

AbstractObwohl noch nicht routinemäßig in der Klinik eingesetzt, hat der “somatische Gentransfer” als kausale Therapie von Erbkrankheiten ein immenses Potenzial. Eine Modellerkrankung für die Etablierung der Gentherapie ist die schwere kombinierte Immundefizienz (SCID) – hier zeigen sich Erfolge, aber auch Probleme.
Humane Antikörper aus Rindern
Authors: Zündorf Ilse , Dingermann Theo 
Published: April 2009

AbstractMit der XenoMouse® oder der HuMAb‐Mouse® wurde der Grundstein gelegt, humane Antikörper gegen bestimmte Antigene zu generieren: Die murinen Immunglobulin‐Gene wurden “einfach” durch die menschlichen Genorte ersetzt. Anschließend konnte man über die klassische Hybridoma‐Technik den gewünschten Antikörper gewinnen. Mit Vectibix® (Panitumumab) ist der erste derartige Antikörper zugelassen. Nun hat man sich am Rind versucht – aber für polyklonale Antikörper.