bluuebunny/crossref_metadata_2025_split huggingface dataset:
Environmental stress and emotional reactivity: an exploratory experience sampling method study
Authors: Wong Corine Sau Man , Chan Wai Chi , Lo Kristen Wing Yan , Chen Eric Yu Hai , Lam Linda Chiu Wa 
Published: April 2024

BackgroundPrevious studies have shown a relationship between environments and mental health. However, limited studies have investigated the impact of environment stress (ES) on emotional reactivity. Our study aimed to fill this gap by examining how daily ES affects momentary emotional reactivity using experience sampling method (ESM).MethodsParticipants were randomly recruited from a prospective cohort study in Hong Kong to participate in a 7-day ESM study. The participants received eight electronic signals daily assessing their ES, positive affect (PA) and negative affect (NA). Participants were categorized into depressed group or control group based on Revised Clinical Interview Schedule. Psychometric properties of the ESM assessment were evaluated. Multilevel linear regression analyzes were conducted to examine the association of ES with PA, NA and the group status of the participants (cases versus controls).ResultsA total of 15 participants with depression and 15 healthy controls were recruited, and 1307 momentary assessments were completed with a compliance rate of 77.8%. The depressed group demonstrated a significant increase in NA in response to ES, while the control group showed a decrease in PA. In addition, the depressed group reported a lower perception of control and interaction with their environment compared to the control group.ConclusionUsing ESM, a valid, reliable, and easy-to-use self-reporting tool, our findings provided valuable insights on the potential mechanisms underlying emotional responses to stressful environments.
H2O2-Induced Oxidative Stress Responses in Eriocheir sinensis: Antioxidant Defense and Immune Gene Expression Dynamics
Authors: He Qinghong , Feng Wenrong , Chen Xue , Xu Yuanfeng , Zhou Jun , Li Jianlin , Xu Pao , Tang Yongkai 
Published: April 2024

Eriocheir sinensis, a key species in China’s freshwater aquaculture, is threatened by various diseases, which were verified to be closely associated with oxidative stress. This study aimed to investigate the response of E. sinensis to hydrogen peroxide (H2O2)-induced oxidative stress to understand the biological processes behind these diseases. Crabs were exposed to different concentrations of H2O2 and their antioxidant enzyme activities and gene expressions for defense and immunity were measured. Results showed that activities of antioxidant enzymes—specificallysuperoxide dismutase (SOD), catalase (CAT), total antioxidant capacity(T-AOC), glutathione (GSH), and glutathione peroxidase (GSH-Px)—varied with exposure concentration and duration, initially increasing then decreasing. Notably, SOD, GSH-Px, and T-AOC activities dropped below control levels at 96 h. Concurrently, oxidative damage markers, including malondialdehyde (MDA), H2O2, and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels, increased with exposure duration. The mRNA expression of SOD, CAT, and GSH-Px also showed an initial increase followed by a decrease, peaking at 72 h. The upregulation of phenoloxidaseloxidase (proPO) and peroxinectin (PX) was also detected, but proPO was suppressed under high levels of H2O2. Heat shock protein 70 (HSP70) expression gradually increased with higher H2O2 concentrations, whereas induced nitrogen monoxide synthase (iNOS) was upregulated but decreased at 96 h. These findings emphasize H2O2’s significant impact on the crab’s oxidative and immune responses, highlighting the importance of understanding cellular stress responses for disease prevention and therapy development.
Analysis of the Energy, Environmental and Economic Efficiency of Multi-Family Residential Buildings in Poland
Authors: Alsabry Abdrahman , Szymański Krzysztof , Backiel-Brzozowska Beata 
Published: April 2024

Improving the energy efficiency of buildings is one of the main challenges facing Europe in the context of climate neutrality policy. In this article, the authors decided to investigate the impact of thermal insulation of building envelopes and the type of ventilation on the energy efficiency of multi-family buildings located in different Polish cities. In accordance with EU directives, economic analyses of the costs incurred during the lifecycle and calculations of CO2 emissions were carried out for the analyzed facilities. It was determined what measures need to be taken under Polish climatic conditions in order to meet the requirements for improving the energy efficiency of multi-family buildings. The multi-criteria analyses presented in this article provide a voice in the discussion of the issues related to the achievement of the energy performance of buildings with almost zero primary energy demand (nZEB). Based on the conclusions of the research conducted, it was possible to develop general recommendations for investors and designers of multi-family residential buildings characterized by energy efficiency and reduced environmental impact.
Integrated Analysis of Transcriptome and Metabolome Reveals Differential Responses to Alternaria brassicicola Infection in Cabbage (Brassica oleracea var. capitata)
Authors: Lei Jinzhou , Zhang Wei , Yu Fangwei , Ni Meng , Liu Zhigang , Wang Cheng , Li Jianbin , Song Jianghua , Wang Shenyun 
Published: April 2024

Black spot, caused by Alternaria brassicicola (Ab), poses a serious threat to crucifer production, and knowledge of how plants respond to Ab infection is essential for black spot management. In the current study, combined transcriptomic and metabolic analysis was employed to investigate the response to Ab infection in two cabbage (Brassica oleracea var. capitata) genotypes, Bo257 (resistant to Ab) and Bo190 (susceptible to Ab). A total of 1100 and 7490 differentially expressed genes were identified in Bo257 (R_mock vs. R_Ab) and Bo190 (S_mock vs. S_Ab), respectively. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that “metabolic pathways”, “biosynthesis of secondary metabolites”, and “glucosinolate biosynthesis” were the top three enriched KEGG pathways in Bo257, while “metabolic pathways”, “biosynthesis of secondary metabolites”, and “carbon metabolism” were the top three enriched KEGG pathways in Bo190. Further analysis showed that genes involved in extracellular reactive oxygen species (ROS) production, jasmonic acid signaling pathway, and indolic glucosinolate biosynthesis pathway were differentially expressed in response to Ab infection. Notably, when infected with Ab, genes involved in extracellular ROS production were largely unchanged in Bo257, whereas most of these genes were upregulated in Bo190. Metabolic profiling revealed 24 and 56 differentially accumulated metabolites in Bo257 and Bo190, respectively, with the majority being primary metabolites. Further analysis revealed that dramatic accumulation of succinate was observed in Bo257 and Bo190, which may provide energy for resistance responses against Ab infection via the tricarboxylic acid cycle pathway. Collectively, this study provides comprehensive insights into the Ab–cabbage interactions and helps uncover targets for breeding Ab-resistant varieties in cabbage.
Green Synthesized Chitosan Nanoparticles for Controlling Multidrug-Resistant mecA- and blaZ-Positive Staphylococcus aureus and aadA1-Positive Escherichia coli
Authors: Rahman Aminur , Kafi Md Abdul , Beak Geunyoung , Saha Sanjay Kumar , Roy Kumar Jyotirmoy , Habib Ahsan , Faruqe Tania , Siddique Mahbubul Pratik , Islam Md. Shafiqul , Hossain Khandker Saadat , Choi Jeong-Woo 
Published: April 2024

Antimicrobial resistance has recently been considered an emerging catastrophe globally. The public health and environmental threats were aggravated by the injudicious use of antibiotics in animal farming, aquaculture, and croup fields, etc. Consequently, failure of antibiotic therapies is common because of the emergence of multidrug-resistant (MDR) bacteria in the environment. Thus, the reduction in antibiotic spillage in the environment could be an important step for overcoming this situation. Bear in mind, this research was focused on the green synthesis of chitosan nanoparticles (ChiNPs) using Citrus lemon (Assam lemon) extract as a cross-linker and application in controlling MDR bacteria to reduce the antibiotic spillage in that sector. For evaluating antibacterial activity, Staphylococcus aureus and Escherichia coli were isolated from environmental specimens, and their multidrug-resistant pattern were identified both phenotypically by disk diffusion and genotypically by detecting methicillin- (mecA), penicillin- (blaZ), and streptomycin (aadA1)-resistance encoding genes. The inhibitory zone’s diameter was employed as a parameter for determining the antibacterial effect against MDR bacteria revealing 30 ± 0.4 mm, 34 ± 0.2 mm, and 36 ± 0.8 mm zones of inhibition against methicillin- (mecA) and penicillin (blaZ)-resistant S. aureus, and streptomycin (aadA1)-resistant E. coli, respectively. The minimum inhibitory concentration at 0.31 mg/mL and minimum bactericidal concentration at 0.62 mg/mL of yielded ChiNPs were used as the broad-spectrum application against MDR bacteria. Finally, the biocompatibility of ChiNPs was confirmed by showing a negligible decrease in BHK-21 cell viability at doses less than 2 MIC, suggesting their potential for future application in antibiotic-free farming practices.
The Putative Cytochrome b5 Domain-Containing Protein CaDap1 Homologue Is Involved in Antifungal Drug Tolerance, Cell Wall Chitin Maintenance, and Virulence in Candida albicans
Authors: Xu Dayong , Wang Manman , Zhang Xing , Mao Hongchen , Xu Haitao , Zhang Biao , Zeng Xin , Li Feng 
Published: April 2024

Candida albicans (Ca), a prominent opportunistic fungal pathogen in humans, has garnered considerable attention due to its infectious properties. Herein, we have identified and characterized CaCDAP1 (Ca orf19.1034), a homolog of ScDAP1 found in Saccharomyces cerevisiae. CaCDAP1 encodes a 183-amino acid protein with a conserved cytochrome b5-like heme-binding domain. The deletion of CaDAP1 renders Ca cells susceptible to caspofungin and terbinafine. CaDAP1 deletion confers resistance to Congo Red and Calcofluor White, and sensitivity to sodium dodecyl sulfate. The deletion of CaDAP1 results in a 50% reduction in chitin content within the cell wall, the downregulation of phosphorylation levels in CaMkc1, and the upregulation of phosphorylation levels in CaCek1. Notably, CaDAP1 deletion results in the abnormal hyphal development of Ca cells and diminishes virulence in a mouse systemic infection model. Thus, CaDAP1 emerges as a critical regulator governing cellular responses to antifungal drugs, the synthesis of cell wall chitin, and virulence in Ca.
Essential Norm of t-Generalized Composition Operators from F(p, q, s) to Iterated Weighted-Type Banach Space
Authors: Alyusof Shams , Hmidouch Nacir 
Published: April 2024

In this work, we characterize the boundedness of t-generalized composition operators from F(p, q, s) spaces to iterated weighted-type Banach space. We also provide estimates of the norm and the essential norm of t-generalized composition operators from F(p, q, s) spaces to iterated weighted-type Banach space. As corollaries, we obtain approximations of the essential norm of integral operators and generalized composition operators from F(p, q, s) spaces to iterated weighted-type Banach space. Moreover, we conclude our work by discussing the t-generalized composition operators and the special cases of F(p, q, s).
Caesarean Section Scar and Placental Location at the First Trimester of Pregnancy—A Prospective Longitudinal Study
Authors: Savukyne Egle , Kliucinskas Mindaugas , Malakauskiene Laura , Berskiene Kristina 
Published: April 2024

Background and Objectives: This study aims to report the location of the placenta in the first trimester of pregnancy in groups of women according to the number of previous caesarean deliveries and the visibility of the caesarean scar niche. Materials and Methods: The prospective observational research included adult women aged 18 to 41 years during pregnancy after one or more previous caesarean sections (CSs). Transvaginal (TVS) and transabdominal sonography (TAS) was used to examine the uterine scar and placental location during 11–14 weeks. The CS scar niche (“defect”) was bordered in the sagittal plane as a notch at the previous CS scar’s site with a depth of 2.0 mm or more. A comparative analysis of the placental location (high or low and anterior or posterior) was performed between groups of women according to the CS number and the CS scar niche. Results: A total of 122 participants were enrolled during the first-trimester screening. The CS scar defect (“niche”) was visible in 40.2% of cases. In cases after one previous CS, the placenta was low in the uterine cavity (anterior or posterior) at 77.4%, and after two or more CSs, it was at 67.9%. Comparing the two groups according to the CS scar niche, the placenta was low in 75.5% of cases in the participant group with a CS scar niche and in 75% of cases without a CS scar niche (p = 0.949). Conclusions: The number of previous caesarean deliveries has no effect on the incidence rate of low-lying placentas in the first trimester. Moreover, the presence of the CS scar niche is not associated with anterior low-lying placentas.
Preoperative prediction of perineural invasion of rectal cancer based on a magnetic resonance imaging radiomics model: A dual-center study
Authors: Liu Yan , Sun Bai-Jin-Tao , Zhang Chuan , Li Bing , Yu Xiao-Xuan , Du Yong 
Published: April 2024

BACKGROUND
Perineural invasion (PNI) has been used as an important pathological indicator and independent prognostic factor for patients with rectal cancer (RC). Preoperative prediction of PNI status is helpful for individualized treatment of RC. Recently, several radiomics studies have been used to predict the PNI status in RC, demonstrating a good predictive effect, but the results lacked generalizability. The preoperative prediction of PNI status is still challenging and needs further study.
AIM
To establish and validate an optimal radiomics model for predicting PNI status preoperatively in RC patients.
METHODS
This retrospective study enrolled 244 postoperative patients with pathologically confirmed RC from two independent centers. The patients underwent pre-operative high-resolution magnetic resonance imaging (MRI) between May 2019 and August 2022. Quantitative radiomics features were extracted and selected from oblique axial T2-weighted imaging (T2WI) and contrast-enhanced T1WI (T1CE) sequences. The radiomics signatures were constructed using logistic regression analysis and the predictive potential of various sequences was compared (T2WI, T1CE and T2WI + T1CE fusion sequences). A clinical-radiomics (CR) model was established by combining the radiomics features and clinical risk factors. The internal and external validation groups were used to validate the proposed models. The area under the receiver operating characteristic curve (AUC), DeLong test, net reclassification improvement (NRI), integrated discrimination improvement (IDI), calibration curve, and decision curve analysis (DCA) were used to evaluate the model performance.
RESULTS
Among the radiomics models, the T2WI + T1CE fusion sequences model showed the best predictive performance, in the training and internal validation groups, the AUCs of the fusion sequence model were 0.839 [95% confidence interval (CI): 0.757-0.921] and 0.787 (95%CI: 0.650-0.923), which were higher than those of the T2WI and T1CE sequence models. The CR model constructed by combining clinical risk factors had the best predictive performance. In the training and internal and external validation groups, the AUCs of the CR model were 0.889 (95%CI: 0.824-0.954), 0.889 (95%CI: 0.803-0.976) and 0.894 (95%CI: 0.814-0.974). Delong test, NRI, and IDI showed that the CR model had significant differences from other models (P < 0.05). Calibration curves demonstrated good agreement, and DCA revealed significant benefits of the CR model.
CONCLUSION
The CR model based on preoperative MRI radiomics features and clinical risk factors can preoperatively predict the PNI status of RC noninvasively, which facilitates individualized treatment of RC patients.
Association between childhood obesity and gut microbiota: 16S rRNA gene sequencing-based cohort study
Authors: Li Xu-Ming , Lv Qing , Chen Ya-Jun , Yan Lu-Biao , Xiong Xin 
Published: April 2024

BACKGROUND
This study aimed to identify characteristic gut genera in obese and normal-weight children (8-12 years old) using 16S rDNA sequencing. The research aimed to provide insights for mechanistic studies and prevention strategies for childhood obesity. Thirty normal-weight and thirty age- and sex-matched obese children were included. Questionnaires and body measurements were collected, and fecal samples underwent 16S rDNA sequencing. Significant differences in body mass index (BMI) and body-fat percentage were observed between the groups. Analysis of gut microbiota diversity revealed lower α-diversity in obese children. Di-fferences in gut microbiota composition were found between the two groups. Prevotella and Firmicutes were more abundant in the obese group, while Bacteroides and Sanguibacteroides were more prevalent in the control group.
AIM
To identify the characteristic gut genera in obese and normal-weight children (8-12-year-old) using 16S rDNA sequencing, and provide a basis for subsequent mechanistic studies and prevention strategies for childhood obesity.
METHODS
Thirty each normal-weight, 1:1 matched for age and sex, and obese children, with an obese status from 2020 to 2022, were included in the control and obese groups, respectively. Basic information was collected through questionnaires and body measurements were obtained from both obese and normal-weight children. Fecal samples were collected from both groups and subjected to 16S rDNA sequencing using an Illumina MiSeq sequencing platform for gut microbiota diversity analysis.
RESULTS
Significant differences in BMI and body-fat percentage were observed between the two groups. The Ace and Chao1 indices were significantly lower in the obese group than those in the control group, whereas differences were not significant in the Shannon and Simpson indices. Kruskal-Wallis tests indicated significant differences in unweighted and weighted UniFrac distances between the gut microbiota of normal-weight and obese children (P < 0.01), suggesting substantial disparities in both the species and quantity of gut microbiota between the two groups. Prevotella , Firmicutes , Bacteroides , and Sanguibacteroides were more abundant in the obese and control groups, respectively. Heatmap results demonstrated significant differences in the gut microbiota composition between obese and normal-weight children.
CONCLUSION
Obese children exhibited lower α-diversity in their gut microbiota than did the normal-weight children. Significant differences were observed in the composition of gut microbiota between obese and normal-weight children.
First Detection of Outflowing Gas in the Outskirts of the Broad-line Region in 1H 0707−495*
Authors: Rodríguez-Ardila Alberto , Fonseca-Faria Marcos Antonio , Dias dos Santos Denimara , Panda Swayamtrupta , Marinello Murilo 
Published: April 2024

Abstract
We use near-infrared spectroscopy covering simultaneously the zJHK bands to look for outflowing gas from the nuclear environment of 1H 0707−495 taking advantage that this region is dominated by low-ionization broad-line region lines, most of them isolated. We detect broad components in H i, Fe ii, and O i, at rest to the systemic velocity, displaying FWHM values of ∼500 km s−1, consistent with its classification as a narrow-line Seyfert 1 active galactic nucleus. Moreover, most lines display a conspicuous blue-asymmetric profile, modeled using a blueshifted component, whose velocity shift reaches up to ∼826 km s−1. This last feature can be interpreted in terms of outflowing gas already observed in X-ray and UV lines in 1H 0707−495 but not detected before in the low-ionization lines. We discuss the relevance of our findings within the framework of the wind scenario already proposed for this source and suggest that the wind extends well into the narrow-line region owing to the observation of a blueshifted component in the forbidden line of [S iii] λ9531.
GN-z11 in Context: Possible Signatures of Globular Cluster Precursors at Redshift 10
Authors: Senchyna Peter , Plat Adele , Stark Daniel P. , Rudie Gwen C. , Berg Danielle , Charlot Stéphane , James Bethan L. , Mingozzi Matilde 
Published: April 2024

Abstract
The first JWST spectroscopy of the luminous galaxy GN-z11 simultaneously established its redshift at z = 10.6 and revealed a rest-ultraviolet spectrum dominated by signatures of highly ionized nitrogen, which has so far defied clear interpretation. We present a reappraisal of this spectrum in the context of both detailed nebular modeling and nearby metal-poor reference galaxies. The N iv] emission enables the first nebular density measurement in an apparently predominantly star-forming galaxy at z > 10, revealing evidence for extremely high densities n

e
≳ 105 cm−3. With a suite of photoionization models, we establish that regardless of the ionization mechanism and accounting for depletion and this density enhancement, gas substantially enriched in nitrogen ([N/O] = +0.52 assuming the nebular emission is dominated by star formation) is required to reproduce the observed lines. We compare the GN-z11 spectrum to local UV databases and highlight a unique nearby galaxy, Mrk 996, where a high concentration of Wolf–Rayet stars and their CNO-processed ejecta produce a UV spectrum remarkably similar in some respects to that of GN-z11 and the Sunburst Arc. Collating this evidence in the context of Galactic stellar abundances, we suggest that the peculiar nitrogenic features prominent in GN-z11 may be a unique signature of intense and densely clustered star formation in the evolutionary chain of the present-day globular clusters, consistent with in situ early enrichment with nuclear-processed stellar ejecta on a massive scale. Combined with insight from local galaxies, these and future JWST data open a powerful new window into the physical conditions of star formation and chemical enrichment at the highest redshifts.