bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mesenchymal stem cells improve osteoarthritis by secreting superoxide dismutase to regulate oxidative stress response
Authors: Yao Yao , Cao Juan , Ding Congzhu 
Published: April 2024

Abstract
Background
To investigate the therapeutic effect of intraarticular injection of mesenchymal stem cells (MSC) in a rabbit osteoarthritis (OA) model. And to suppose whether MSC play a pivotal role in OA therapy by improving oxidative stress through secreting superoxide dismutase (SOD).
Methods
MSC were isolated and cultured in vitro. SOD gene of MSC was silenced by siRNA technology to prepare the SOD-siRNA-MSC for in-vivo study. Twenty healthy adult New Zealand white rabbits underwent papain injection to induce OA and then received intra-articular injection with MSC, siRAN-MSC, or normal saline. The rabbits were divided into 4 groups (n = 5), such as the control group, the model group, the MSC group, the siRNA-MSC group. Cytokines determination was performed 2 and 4 weeks after treatment. Magnetic resonance imaging (MRI) and histopathology and immunohistochemistry determination were performed 4 weeks after treatment.
Results
COMP, TNF-α, MMP-2 and MMP-13 in the MSC group were significantly decreased compared to those in model group (P < 0.05). However, MMP2 and MMP13 in the siRNA-MSC group were not significantly decreased compared to the model group (P < 0.05). Magnetic resonance results revealed a significant improvement in cartilage and synovial membrane 4 weeks after MSC injection. Histopathology determination showed that cartilage structure was also significantly improved in MSC group. Immunohistochemical analysis revealed amelioration in the expression levels of proteoglycan, COL-2, P21 and P53 in MSC group. On the other hand, MRI, histopathologic and immunohistochemical analysis also indicated a decreased therapeutic effect with SOD-siRNA -MSC.
Conclusion
Our study demonstrated for the first time that MSC might be a promising therapy in OA through anti-apoptosis and regeneration in chondrocyte by secreting SOD and improving oxidative stress.
Wheat Growth and Yield in the Rice -Wheat Cropping System: Impact of Crop Establishment Techniques, Sowing Schedule and Nitrogen management
Authors: Chhabra Vandna , Sree S , Kaur Gurleen 
Published: April 2024

Abstract

A two-year field experiment was conducted at Lovely Professional University, Phagwara during the Rabi season of 2018–2019 and 2019-20 to study the effect of crop establishment methods and sowing schedule on the growth and yield of wheat under the rice-wheat cropping system. The experiment comprised different rice residue management-based wheat establishment methods, sowing dates, and varying nitrogen levels. The results confirmed that growth, yield and yield attributes were all influenced by different crop establishment methods, sowing dates and level of nitrogen application. The results showed that maximum plant height, number of tillers per plant, number of spikes per plant, spike length, number of grains per spike and grain yield were observed under 20th November sowing (timely sowing) and with 100% RDN. Moreover, the maximum number of spikes per plant, spike length and grain yield were significantly highest for residue incorporation method of wheat establishment. Optimum time of sowing also improved the grain yield of wheat. Among the different crop establishment methods, the best attainment of available nitrogen status in soil was found in residue incorporation treatment.
Fjords, Echinoderms and the Holocene: a new ecological proxy from the Ross Sea (Edisto Inlet)
Authors: Galli Giacomo , Morigi Caterina , Thuy Ben , Gariboldi Karen 
Published: April 2024

Abstract
High Latitude fjords can serve as sediment trap, bearing different type of proxies, from geochemical to micropaleontological ones, making them exceptional tools for paleoenvironmental reconstruction. However, some unconventional proxies can be present and can be used to depict a comprehensive and exhaustive interpretation of past changes. Here, studying a sediment core in Edisto Inlet (Ross Sea, Antarctica) we used irregular echinoid spines and ophiuroids (Ophionotus victoriae) ossicles to trace environmental changes throughout the last 3.6 kyrs BP. Irregular echinoids can serve as proxy for the organic matter content, while O. victoriae ossicles can be used as proxy for steady sea-ice cycle along with organic deposition events. O. victoriae release a high number of ossicles, making estimation about the population quite challenging; still, presence data, can be easily collected. By applying Generative Additive Models to the stratigraphical distribution of these data, we detected an environmental phase that was previously unnoticed by other traditional proxies: the Ophiuroid Optimum (2-1.5 kyrs BP). In conclusion, here we demonstrate how echinoderm presence can be used as a valuable source of information, while proving the potential of modelling binary data to detect long-term trend in Holocene stratigraphical records.
Participatory environmental management beyond the boundaries of Protected Areas: a democratic advancement or nature privatization? A systematic review
Authors: Souza Luísa Couto Gonçalves , Damásio Camila Braga , Andrade Lívia Maria Comini , Lamounier Wanderson Lopes , Drumond Maria Auxiliadora 
Published: April 2024

Abstract

In recent decades, environmental management has undergone changes that have dispersed decision-making power, from a model centralized in public authorities to one with power also distributed to other stakeholders, such as community members, companies, and non-governmental organizations. Participatory environmental management favors conflict reduction, increased legitimacy and compliance with decisions, and community empowerment, fostering more effective and equitable socio-environmental management. However, conciliation of divergent interests, the need for greater investments in time, logistics, capacity building, and power asymmetries are possible limitations to the outcomes of this model, enabling its use in favor of minority interests, especially outside Protected Areas. Numerous studies have been developed on the subject, but its relevance demands an analytical compilation of these works. This review aims to fill this gap by critically synthesizing participatory environmental management case studies in territories outside the Protected Areas, extracted from 145 systematically selected publications. The analysis of aspects such as study location, management group format, level of participation of civil society and traditional communities, factors influencing participation, financial support, difficulties, strategies, and outcomes indicates that, in many cases, the diversity of stakeholders has turned the decentralization of power into an aggravating factor for the vulnerability of weaker parties. Nevertheless, observed advancements in participation corroborate the feasibility and importance of inclusive management. This review provides a current, integrated, and critical overview, useful for the global understanding of the subject and studies improvement and participatory processes in the planning or execution phase.
Combined ecological-chemical-pharmacological analysis of the ecological suitability and quality zoning of the Tinosporae Radix
Authors: Huang Chengfeng , Lu Lijie , Zhu Xinglong , Huang Xulong , Zhou Yongfeng , Tang Linye , Du Jinyu , Pei Jin , Wu Qinghua 
Published: April 2024

Abstract

The Tinosporae Radix is favored as a treatment for pharyngitis and is now regarded as the first choice of traditional Chinese medicine as an alternative to antibiotics. However, its wild resources are on the verge of extinction due to over-excavation by human beings, and the quality of the cultivated products varies, so it is urgent to search for the key biological factors affecting the growth and development process of Tinosporae Radix as well as the suitable ecological zones. In this study, we used MaxEnt model and Arcgis spatial analysis technology to predict the suitable growing area of Tinosporae Radix, and found four key environmental factors affecting the quality of Tinosporae Radix. In this paper, we used UPLC analysis to obtain the fingerprints of Tinosporae Radix, and found that the most relevant components for the anti-inflammatory effect of Tinosporae Radix included columbin and several alkaloids, and the correlation analysis with the relevant biotic factors revealed that Tinosporae Radix is suitable for growing in Southwest China, and that Tinosporae Radix with higher content of columbin is suitable for growing in the region of the Yunnan-Guizhou Plateau, and that Tinosporae Radix with higher content of alkaloids is suitable for growing in the borders between Guangxi, Yunnan, Guizhou, and Hunan, which are all characterized by karst landscapes. And all these places are dominated by karst landscape. In vitro analysis of anti-inflammatory efficacy showed that the anti-inflammatory efficacy was good regardless of the high content of either type of components. In this paper, we evaluated the quality of Tinosporae Radix herbs in the suitable habitat obtained by model prediction through the method of "chemical evaluation" associated with "anti-inflammatory" efficacy, and obtained an innovative "ecological-chemical-pharmacological efficacy" evaluation method, which is a good method to evaluate the quality of Tinosporae Radix herbs in the suitable habitat. The results of this method can be used to guide the production of Tinosporae Radix with a high content of columbin or alkaloids, laying the foundation for the establishment of standardized artificial cultivation of Tinosporae Radix, and at the same time, providing theoretical support for the delineation of Tinosporae Radix's suitable areas, the utilization of its resources, and the extension of the industrial chain.
Quantification and profiling of early and late differentiation stage T-cells in mantle cell lymphoma reveals immunotherapeutic targets in subsets of patients
Authors: Lokhande Lavanya , Nilsson Daniel , Rodrigues Joana de Matos , Hassan May , Olsson Lina M , Pyl Paul-Theodor , Vasquez Louella , Porwit Anna , Gerdtsson Anna Sandstrom , Jerkeman Mats , Ek Sara 
Published: April 2024

Abstract
Background
The response to immune checkpoint inhibition has been limited in mantle cell lymphoma (MCL), and the association to variation in abundance and molecular profile of different T-cell subsets is underexplored. With the aim to advance understanding of immune regulation in MCL and to identify targetable T-cell subsets in patient subgroups, we set out to combine image analysis, spatial omic technology focused on both early and late differentiation stages of T-cells.
Methods
A population-based cohort of 102 MCL patients was available for image analysis and GeoMx spatial omics profiling of 69 proteins and 1812 mRNAs. CD20, CD3, CD8 and CD57 were used to identify tumor cells, T helper (TH) and cytotoxic (TC) cells of early (CD57-) and late (CD57+) differentiation stage. An image analysis workflow was developed based on fine-tuned CellPose models for cell segmentation and classification. Cell frequencies and spatial omics data was collected in tumor-rich regions (cells in MCL-dominated regions) and tumor-sparse (tumor-adjacent T-cell rich regions with no/few tumor cells).
Results
Both TC and CD57 + subsets were enriched in tumor-rich compared to tumor-sparse regions. Tumor-sparse regions had higher expression of several key tumor suppressive proteins, tentatively controlling T-cell expansion in regions close to the tumor. Comparison between individual subsets of T-cells (TH,57, TC,57−, TH,57+, TC,57+) infiltrating the MCL regions, showed that CD57 + late differentiation stage T-cells were associated with expression of immune inhibitory molecules such as TIGIT, PD-L1, PD-L2, and LAG3. CD47 and IDO1 expression on tumor cells was associated with T-cell rich MCL, while GITR was higher expressed in T-cell sparse MCL.
Conclusions
Through combined image analysis and spatial omics, we revealed that T-cells in late differentiation stages (CD57+) are enriched among MCL infiltrating T-cells and are predictive of increased expression of immune suppressive markers. CD47, IDO1 and CTLA-4 were identified as potential targets for patients with T-cell rich MCL TIME, while MCL patients with sparse T-cell infiltration may benefit from targeting GITR. In subgroups of patients with high degree of CD57 + TC-cell infiltration several immune checkpoint inhibitors, including TIGIT, PD-L1 and LAG3 were increased, emphasizing the immune-suppressive features of this T-cell subsets not previously described in MCL.
Weight Loss-induced Interaction Between Classical Lipolysis and the Autolysosome in Human Subcutaneous Adipose Tissue
Authors: Mariman Edwin , Baak Marleen van , Bouwman Freek 
Published: April 2024

Abstract

Background/objectives:During a period of weight loss lipolysis genes in human subcutaneous adipose tissue are downregulated despite the increase in plasma free fatty acids. It has been proposed that lipid breakdown is taken over by the autolysosome. Here we test the relation between lipolysis and the autolysosome.
Subjects/methods:Gene and protein expression data from the YoYo-study were used for correlation analysis including genes coding for lipases and regulators of lipolysis, for autolysosome proteins and lysosomal enzymes, and the genes coding for components of a previously identified integrin cluster. For all these genes the cell type and compartment of expression was obtained from databases. Correlation analysis was performed using the gene expression values before weight loss (WL), after WL, and after a subsequent weight stable period (WS), and using the expression changes during WL and WS.
Results:During WL a significant negative correlation originated between the lipolysis and autolysosome genes. Genes of the integrin cluster correlated negative with the lipolysis genes and positive with the autolysosome genes. Surprisingly, the lipolysis genes were expressed in mature adipocytes while the autolysosome genes were not, but were expressed in other types of cells of the adipose tissue. Most of the correlated autolysosome genes were secreted or on the plasma membrane. After WL most of the genes reversed their direction of expression. During WS the correlation between lipolysis and autolysosome genes lost significance and the correlation with the integrin genes disappeared.
Conclusions:Our findings do not support a transfer of lipid breakdown from lipolysis to the autolysosome in subcutaneous adipocytes during WL. Instead, we observe an intercellular interaction between lipolysis in mature adipocytes and the autolysosome of other cells in the adipose tissue. This interaction is probably mediated by integrin-mediated contact between the cells. A model for the observed interaction based on ECM-stress is presented.
Improving Post-Injury Follow-up Survey Response: Incorporating Automated Modalities
Authors: Scheuer Hannah , Conrick Kelsey M. , Mills Brianna , Solano Esther , Arbabi Saman , Bulger Eileen M. , Dotolo Danae , Vil Christopher St. , Vavilala Monica S. , Rowhani-Rahbar Ali , Moore Megan 
Published: April 2024

Abstract

Background
Incorporating post-discharge data into trauma registries would allow for better research on patient outcomes, including disparities in outcomes. This pilot study tested a follow-up data collection process to be incorporated into existing trauma care systems, prioritizing low-cost automated response modalities.
Methods
This investigation was part of a larger study that consisted of two protocols with two distinct cohorts of participants who experienced traumatic injury. Participants in both protocols were asked to provide phone, email, text, and mail contact information to complete follow-up surveys assessing patient-reported outcomes six months after injury. To increase follow-up response rates between protocol 1 and protocol 2, the study team modified the contact procedures for the protocol 2 cohort. Frequency distributions were utilized to report the frequency of follow-up response modalities and overall response rates in both protocols.
Results
A total of 178 individuals responded to the 6-month follow-up survey: 88 in protocol 1 and 90 in protocol 2. After implementing new follow-up contact procedures in protocol 2 that relied more heavily on the use of automated modalities (e.g., email and text messages), the response rate increased by 17.9 percentage points. The primary response modality shifted from phone (72.7%) in protocol 1 to the combination of email (47.8%) and text (14.4%) in protocol 2.
Conclusions
Results from this investigation suggest that follow-up data can feasibly be collected from trauma patients. Use of automated follow-up methods holds promise to expand longitudinal data in the national trauma registry and broaden the understanding of disparities in patient experiences.
The Benefit of Active Management in True Knot of the Umbilical Cord: A retrospective study.
Authors: Weissbach Tal , Lev Shir , Back Yonatan , Massarwa Abeer , Meyer Raanan , Miller Tal Elkan , Weissmann-Brenner Alina , Weisz Boaz , Mazaki-Tovi Shali , Kassif Eran 
Published: April 2024

Abstract

Purpose: To compare perinatal outcomes between active and routine management in true knot of the umbilical cord (TKUC).
Methods: A retrospective study of singletons born beyond 22 6/7 weeks with TKUC. Active management included weekly fetal heart rate monitoring(FHRM)≥ 30 weeks and labor induction at 36-37 weeks. Outcomes in active and routine management were compared, including composite asphyxia-related adverse outcome, fetal death, labor induction, Cesarean section (CS) or Instrumental delivery due to non-reassuring fetal heart rate (NRFHR), Apgar5 score <7, cord Ph <7, neonatal intensive care unit (NICU) admission and more.
Results: The Active (n=59) and Routine (n=1091) Management groups demonstrated similar rates of composite asphyxia-related adverse outcome (16.9% vs 16.8%, p= 0.97). Active Management resulted in higher rates of labor induction<37 weeks (22% vs 1.7%, p<0.001), CS (37.3% vs 19.2%, p=0.003) and NICU admissions (13.6% vs 3%, p<0.001). Fetal death occurred exclusively in the Routine Management group (1.8% vs 0%, p=0.6).
Conclusion: Compared with routine management, weekly FHRM and labor induction between 36-37 weeks in TKUC do not appear to reduce neonatal asphyxia. In its current form, active management is associated with higher rates of CS, induced prematurity and NICU admissions. Labor induction before 37 weeks should be avoided.
Financial toxicity in patients with chronic kidney disease undergoing peritoneal dialysis
Authors: kong hanhan , Li Huanhuan , Liao Yumei , Peng Wujian , Liu Yanqun , Gong Claire , Bai Jinbing , Zhang Liping 
Published: April 2024

Abstract

Aim: To investigate financial toxicity (FT) and its influencing factors in patients with chronic kidney disease undergoing peritoneal dialysis (PD).
Methods: A cross-sectional study was conducted on 148 PD patients recruited between August 2023 and December 2023. The Comprehensive Score for financial Toxicity (COST) was used to estimate the prevalence and degree of FT. Patient coping actions were investigated.T-test, ANOVA, Pearson’s chi-squared test and multiple linear regression analysis were used to evaluate the predictors of FT in patients with PD.
Results: 79 patients (53.38%) had mild FT, and 61 patients (41.21%) had moderate FT. Age, working status, monthly household income per capita and PD-related complications were significantly associated with FT in PD patients (p＜0.05). 115 patients (77.70%) reduced consumption due to illness, 67 patients (45.27%) had medical loans, 58 patients (39.19%) discussed lower-cost treatment plans with their physician, and 40 patients (27.03%) considered abandoning or delaying treatment due to financial difficulties. Patients with higher FT had lower living expenses, higher risk of debt, and were more likely to abandon or delay treatment than patients with lower FT(p＜0.05).
Conclusions: There were varying degrees of FT in PD patients. Measuring FT can help medical professionals develop treatment care strategies, reduce the financial burden on patients, and avoid treatment interruption.
Early-stage acral melanoma with a history of trauma associated with a poor prognosis:A multicenter data analysis of 468 cases
Authors: 滕 熠 , Wu Jin , Cai Xin , Zhang Weizhen , Jiang Kui , Zhou Hongfeng , Guo Zhen , Liu Jiwei , Wang Yan , Liu Fang , Lan Shijie , Meng Hongxue , Ji Xiang , Xiang Mei Mei , Li Yongqi , Wu Di 
Published: April 2024

Abstract
Background：Previous studies have suggested that trauma may be a risk factor for the development and prognosis of acral melanoma (AM), but the available evidence is limited.This study aimed to explore the impact of trauma on AM recurrence and survival.
Methods：This population-based retrospective cohort study included patients with acral melanoma with a clear history of trauma who were treated at five melanoma treatment centers in China from 2009 to 2023. Data were analyzed July 2023 to August 2023. Baseline characteristics of the two groups were analyzed using the chi-square test. Disease-free survival (DFS) and overall survival (OS) were analyzed using the Kaplan-Meier method. Factors associated with recurrence and survival were determined using univariate and multivariate Cox regression analysis.
Results：A total of 468 AM patients were included in this study, with 101 in the trauma group and 367 in the non-trauma group. In the overall population, the median follow-up time was 57.4 months (95%CI, 53.84-61.03), with 248 (53.0%) males and 220 (47.0%) females. The peak age of onset was between 60-69 years old (31.2%) . In the trauma group, the plantars area was the most frequently affected site (54.5%), followed by the hallux toe (17.8%) and thumb (9.9%). The proportion of patients with ulcer (P=0.027) , mitosis rate ≥1 (P=0.036), and Clark level IV-V (P=0.009) was significantly higher in the trauma group compared to the non-trauma group. Multivariate analysis demonstrated that trauma was an independent risk factor for both DFS and OS in stage I-II. Furthermore, the cumulative survival rate revealed that the I-II trauma group had significantly lower than the non-trauma group.
Conclusions：The results of this cohort study showed that trauma was found to be an independent risk factor for DFS in AM patients with stage I-II, affecting DFS and OS in AM.
Construction of Larger Dimensional Hulls of BCH Codes with Length $n=\frac{q^4-1}{q+1}$
Authors: Yan Ming , Yan Tongjiang , Sun Yuhua 
Published: April 2024

Abstract

Hulls of linear codes are widely applied in constructing self-orthogonal codes and quantum codes, as well as in various other aspects. Let $\mathbb{F}_q$ be the finite field of order $q$ and $n=\frac{q^4-1}{q+1}$, where $q$ is an odd prime power. Let $\mathcal{C}$ be a BCH code over $\mathbb{F}_q$ with length $n$ and dimension $k$. In this paper, we provide the necessary and sufficient conditions for $\mathcal{C}$ possessing a hull with dimension $k-1$ or $k^{\perp}-1$, where $k^{\perp}$ is the dimension of the dual code of $\mathcal{C}$, while also deriving several classes of self-orthogonal codes, including some optimal codes.