bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gypsy Policy in Socialist Hungary and Czechoslovakia, 1945–1989
Authors: McCagg William O. 
Published: September 2011

In discussion of ethnic minorities in Eastern Europe, one hears regularly of appalling official misbehavior—not just about attempted genocide (though that too), but also about bureaucratic cruelties inflicted in every field of human activity and at every level of control. Nonetheless, it is always useful to have a measurable basis for assessing unfairness; and historians have the special task of inquiring rationally why and how unfairness came about. Hence the following paper, which attempts not just to condemn, but to explain and evaluate the Hungarian and Czechoslovak official treatment of the Gypsies in recent decades. As is fairly well known, this treatment has included not only harassment of populations which presently exceed 600,000 people in each country, but also (in both countries) systematic abduction of children by the state from unwilling Gypsy parents, and (in Czechoslovakia) equally systematic sterilization of Gypsy women.Since the point of the paper is to reach beyond mere indictment, I will use a comparative method. Specifically, in recounting each stage of the development of policy towards the Gypsies I will compare what was being done to two other groups: the Jews, on the one hand, and the physically disabled on the other.
The RomaniPořajmos:The Nazi Genocide of Europe's Gypsies
Authors: Huttenbach Henry R. 
Published: September 2011

The Nazi assault on Gypsies as an undesirable group was launched in the first months of the Third Reich. By the end of 1933, the outlines of a policy of total removal and, if possible, extinction were in place. Over the course of the first year of Hitler's rule, Gypsies had been numbered among those destined for mass sterilization. The goal of preventing their propagation had been pronounced on July 14 when the new cabinet issued a statement (with the force of law) proclaiming the concept ofLebensunwertesleben—life unworthy of living—a category of person that, at the time, specifically and indiscriminately included and embraced all Gypsies. Shortly thereafter, exploratory contacts were made with the League of Nations to assess the practicability of allocating one or two Polynesian islands to which the Gypsies could be deported. By September 1933, the Ministry of Interior announced a more realizable preliminary plan to arrest persons with no fixed and permanent addresses (i.e. primarily Gypsies) and to incarcerate them in special detention camps as a means of removing them from the mainstream of society. There the Gypsies would be rendered preemptively criminally harmless, (since they were described as a potential detriment to the general German population), and biologically “futureless”(zukunftloss)by way of mass sterilization.In retrospect, the central ingredients for a formula of genocide, for the complete extermination of the Gypsies, were all in place: an ideology which deprived them of the basic right to life; a process of law by edict, which subjected them to totalitarian rule; a hypothetical plan to deport them abroad, and a more concrete one to isolate them from the citizenry, by segregating them in prison-like compounds, deprived of all civil rights; and a technology of physical mutilation that would deny them progeny and a link with a biological future, by literally destroying the unconceived next generation. Thus, a skeletal blueprint for the genocide of Gypsies by the racial architects of the Nazi regime had been drawn up by the end of 1933 well before the first Gypsies in Germany were rounded up in January 1934.
Foreign Policy
Authors: Vares Peeter , Zurjari Olga 
Published: September 2011

First VisionsDuring the Soviet period Estonia, like the other national republics of the USSR, lacked a foreign policy of her own. While foreign ministries did exist, they had just a symbolic function: staffed by only five or six people, they were allowed minimal cultural and trade contacts with the Western countries and limited inter-communist party ties within the Soviet bloc. They had to report to the USSR Ministry of Foreign Affairs on every move they made and served, first and foremost, as cover organizations for the KGB. Designing more substantive foreign policies in the Baltic Republics actually began before they gained independence in 1991. In 1989–1990, the emerging political parties voiced their first visions of the future of the Baltic States, which, generally speaking, boiled down to becoming sovereign democratic states, striving for friendly relations with all countries of the world. By that time, under the pressures of perestroika and glasnost, the Soviet authorities had been compelled to loosen their grip on the foreign contacts of the union republics. Those contacts, however, could not be called yet a foreign policy. They could, rather, be identified as isolated moves in the arena of international politics.
Deafness, Spelling and Rhyme: How Spelling Supports Written Word and Picture Rhyming Skills in Deaf Subjects
Authors: Campbell Ruth , Wright Helen 
Published: September 2011

Orally trained, congenitally deaf adolescents and hearing, reading-age-matched control subjects made rhyme judgements for pictures and for written words. Hearing children performed the task accurately. By contrast, the deaf group were very poor at rhyme judgement for words and for pictures. For hearing children, word rhyme judgement was more accurate when the words were congruent in their spelling pattern (e.g. bat/hat), less accurate when the spelling pattern of the rhyming words was incongruent ( hair/bear). Deaf subjects showed an even more pronounced effect of spelling congruence; their ability to match for rhyme when written words did not share the same spelling pattern was extremely poor. Moreover, spelling congruence predicted deaf subjects’ picture rhyming skills. We conclude that oral training for deaf people does not always permit them to achieve a reliable phonological representation of speech from lip-reading and residual hearing alone. Instead they use the written spelling of the word. This result is not predicted from some previous results that suggest that orally trained deaf people can make direct, spontaneous use of rhyme in the processing of visually presented material.
Everyday Reasoning with Conditional Sequences
Authors: Byrne Ruth M. J. 
Published: September 2011

Everyday conditional reasoning commonly occurs in a sequence, such as: … If she leaves home early today then she will get to work quickly. If she gets to work quickly then she will get extra things done … Experimental studies of conditional reasoning assume that people make the same amount of inferences from sequences as they do from simple arguments that contain just a single conditional premise. In a series of four experiments this assumption is shown to be wrong. Our first experiment showed that people make more inferences from transitive sequences, and fewer inferences from certain atransitive sequences, than from corresponding simple arguments. We established in the second experiment that these differences arise because people can construct a single representation of a sequence. The third experiment replicated the result that people make fewer inferences from certain atransitive sequences. In the final experiment we found that people make more inferences from transitive sequences, regardless of the presence of negatives. We argue that the inferences people make from common everyday sequences depends on their representation of the state of affairs that sequences describe.
Immunogenicity of Recombinant Classic Swine Fever Virus CD8+T Lymphocyte Epitope and Porcine Parvovirus VP2 Antigen Coexpressed by Lactobacillus casei in Swine via Oral Vaccination
Authors: Xu Yigang , Cui Lichun , Tian Changyong , Zhang Guocai , Huo Guicheng , Tang Lijie , Li Yijing 
Published: September 2011

ABSTRACTClassical swine fever virus (CSFV) and porcine parvovirus (PPV) are highly contagious pathogens, resulting in enormous economic losses in pig industries worldwide. Because vaccines play an important role in disease control, researchers are seeking improved vaccines that could induce antiviral immune responses against CSFV and PPV at the mucosal and systemic levels simultaneously. In this study, a genetically engineeredLactobacillusstrain coexpressing the CSFV-specific cytotoxic T lymphocyte (CTL) epitope 290 and the VP2 antigen of PPV was developed, and its immunopotentiating capacity as an oral vaccine in pigs was analyzed. The data demonstrated that in the absence of any adjuvant, the recombinantLactobacillusstrain can efficiently stimulate mucosal and systemic CSFV-specific CD8+CTL responses to protect pigs against CSFV challenge. Moreover, anti-PPV-VP2 serum IgG and mucosal IgA were induced in pigs immunized orally with the recombinantLactobacillusstrain, showing a neutralizing effect on PPV infection. The results suggest that the recombinantLactobacillusmicroecological agent may be a valuable component of a strategy for development of a vaccine against CSFV and PPV.
SmaI Restriction Site-Based Multiplex PCR for Typing of Hospital- and Community-Acquired Staphylococcus aureus
Authors: Al-Zahrani Ibrahim A. , Hamson Clare , Edge David , Collins Jennifer , Perry John D. , Raza Muhammad , Gould Kate , Harwood Colin R. 
Published: September 2011

ABSTRACT

Methicillin-resistant
Staphylococcus aureus
(MRSA) is an important nosocomial pathogen, and morbidity and mortality rates associated with this pathogen have increased markedly in recent years. MRSA strains are generally resistant to several classes of antibiotics and are therefore difficult and costly to treat. A major issue is to identify the sources of MRSA infections and to monitor their epidemic spread. In this study, we report the development of a typing technique for
S. aureus
, based on single-nucleotide polymorphism (SNP) variations in and around SmaI-restriction sites (CCCGGG). An assessment of the SmaI restriction site-based multiplex PCR (SmaI-multiplex PCR) typing (SMT) with respect to pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) revealed a high level of concordance in the clustering of the test strains. The SmaI-multiplex PCR was found to be more discriminatory than MLST/staphylococcal cassette chromosome
mec
(SCC
mec
) typing but less discriminatory than PFGE. SMT can provide real-time information for the investigation of ongoing
S. aureus
hospital outbreaks. SMT meets the criteria of a practical typing method: it is simple, reproducible, and highly discriminatory and does not require expensive equipment or specialist expertise. Consequently, SmaI-multiplex PCR has the potential to be used in routine clinical microbiology laboratories.
Target-Specific Capture Enhances Sensitivity of Electrochemical Detection of Bacterial Pathogens
Authors: Patel Mayank , Gonzalez Rodrigo , Halford Colin , Lewinski Michael A. , Landaw Elliot M. , Churchill Bernard M. , Haake David A. 
Published: September 2011

ABSTRACT
We report the concentration and purification of bacterial 16S rRNA by the use of a biotinylated DNA target-specific capture (TSC) probe. For both cultivated bacterial and urine specimens from urinary tract infection patients, TSC resulted in a 5- to 8-fold improvement in the sensitivity of bacterial detection in a 16S rRNA electrochemical sensor assay.
The Cellular TAR RNA Binding Protein, TRBP, Promotes HIV-1 Replication Primarily by Inhibiting the Activation of Double-Stranded RNA-Dependent Kinase PKR
Authors: Sanghvi Viraj R. , Steel Laura F. 
Published: September 2011

ABSTRACT

The TAR RNA binding protein, TRBP, is a cellular double-stranded RNA (dsRNA) binding protein that can promote the replication of HIV-1 through interactions with the viral TAR element as well as with cellular proteins that affect the efficiency of translation of viral transcripts. The structured TAR element, present on all viral transcripts, can impede efficient translation either by sterically blocking access of translation initiation factors to the 5′-cap or by activating the dsRNA-dependent kinase, PKR. Several mechanisms by which TRBP can facilitate translation of viral transcripts have been proposed, including the binding and unwinding of TAR and the suppression of PKR activation. Further, TRBP has been identified as a cofactor of Dicer in the processing of microRNAs (miRNAs), and sequestration of TRBP by TAR in infected cells has been proposed as a viral countermeasure to potential host cell RNA interference-based antiviral activities. Here, we have addressed the relative importance of these various roles for TRBP in HIV-1 replication. Using Jurkat T cells, primary human CD4
+
T cells, and additional cultured cell lines, we show that depletion of TRBP has no effect on viral replication when PKR activation is otherwise blocked. Moreover, the presence of TAR-containing mRNAs does not affect the efficacy of cellular miRNA silencing pathways. These results establish that TRBP, when expressed at physiological levels, promotes HIV-1 replication mainly by suppressing the PKR-mediated antiviral response, while its contribution to HIV-1 replication through PKR-independent pathways is minimal.
A Sequence within the Varicella-Zoster Virus (VZV) OriS Is a Negative Regulator of DNA Replication and Is Bound by a Protein Complex Containing the VZV ORF29 Protein
Authors: Khalil Mohamed I. , Arvin Ann , Jones Jeremy , Ruyechan William T. 
Published: September 2011

ABSTRACT
The architecture of the varicella-zoster virus (VZV) origin of DNA replication (OriS) differs significantly from that of the herpes simplex virus (HSV) DNA replication origin. Novel aspects of the VZV OriS include a GA-rich region, three binding sites for the VZV origin-binding protein (OBP) all on the same strand and oriented in the same direction, and a partial OBP binding site of unknown function. We have designated this partial binding site Box D and have investigated the role it plays in DNA replication and flanking gene expression. This has been done with a model system using a replication-competent plasmid containing OriS and a replication- and transcription-competent dual-luciferase reporter plasmid containing both the OriS and the intergenic region between VZV open reading frames (ORFs) 62 and 63. We have found that (i) Box D is a negative regulator of DNA replication independent of flanking gene expression, (ii) the mutation of Box D results in a decrease in flanking gene expression, thus a sequence within the VZV OriS affects transcription, which is in contrast to results reported for HSV-1, (iii) there is a specific Box D complex formed with infected cell extracts in electrophoretic mobility shift assay experiments, (iv) supershift assays show that this complex contains the VZV ORF29 single-strand DNA-binding protein, and (v) the formation of this complex is dependent on the presence of CGC motifs in Box D and its downstream flanking region. These findings show that the VZV ORF29 protein, while required for DNA replication, also plays a novel role in the suppression of that process.
A Novel Borna Disease Virus Vector System That Stably Expresses Foreign Proteins from an Intercistronic Noncoding Region
Authors: Daito Takuji , Fujino Kan , Honda Tomoyuki , Matsumoto Yusuke , Watanabe Yohei , Tomonaga Keizo 
Published: September 2011

ABSTRACT
Borna disease virus (BDV), a nonsegmented, negative-strand RNA virus, infects a wide variety of mammalian species and readily establishes a long-lasting, persistent infection in brain cells. Therefore, this virus could be a promising candidate as a novel RNA virus vector enabling stable gene expression in the central nervous system (CNS). Previous studies demonstrated that the 5′ untranslated region of the genome is the only site for insertion and expression of a foreign gene. In this study, we established a novel BDV vector in which an additional transcription cassette has been inserted into an intercistronic noncoding region between the viral phosphoprotein (P) and matrix (M) genes. The recombinant BDV (rBDV) carrying green fluorescent protein (GFP) between the P and M genes, rBDV P/M-GFP, expressed GFP efficiently in cultured cells and rodent brains for a long period of time without attenuation. Furthermore, we generated a nonpropagating rBDV, ΔGLLP/M, which lacks the envelope glycoprotein (G) and a splicing intron within the polymerase gene (L), by the transcomplementation system with either transient or stable expression of the G gene. Interestingly, rBDV ΔGLLP/M established a persistent infection in cultured cells with stable expression of GFP in the absence of the expression of G. Using persistently infected rBDV ΔGLLP/M-infected cells, we determined the amino acid region in the cytoplasmic tail (CT) of BDV G important for the release of infectious rBDV particles and also demonstrated that the CT region may be critical for the generation of pseudotyped rBDV having vesicular stomatitis virus G protein. Our results revealed that the newly established BDV vector constitutes an alternative tool not only for stable expression of foreign genes in the CNS but also for understanding the mechanism of the release of enveloped virions.
Analysis of Single-Nucleotide Polymorphisms in Patient-Derived Retrovirus Integration Sites Reveals Contamination from Cell Lines Acutely Infected by Xenotropic Murine Leukemia Virus-Related Virus
Authors: Rusmevichientong Alice , Das Gupta Jaydip , Elias Petra S. , Silverman Robert H. , Chow Samson A. 
Published: September 2011

ABSTRACT
We analyzed xenotropic murine leukemia virus-related virus (XMRV) integration site sequences previously identified from human prostate tissues for single-nucleotide polymorphisms (SNPs) to discriminate between patient and potential cell line sources of the proviruses. The SNPs of two integration sites were identical to those in cell lines but not the patients, whereas the data on the remaining 12 integration sites were inconclusive. Our results provide direct evidence for contamination during analysis of XMRV integration sites.
Resveratrol-Mediated Gamma Interferon Reduction Prevents Airway Inflammation and Airway Hyperresponsiveness in Respiratory Syncytial Virus-Infected Immunocompromised Mice
Authors: Zang Na , Xie Xiaohong , Deng Yu , Wu Shengde , Wang Lijia , Peng Caijing , Li Simin , Ni Ke , Luo Yan , Liu Enmei 
Published: September 2011

ABSTRACT
Respiratory syncytial virus (RSV) is the most important cause of severe, lower respiratory tract infections in infants, and RSV infections have been associated with chronic wheezing and asthma during childhood. However, the mechanism of RSV-induced airway inflammation and airway hyperresponsiveness (AHR) is poorly understood. Furthermore, there are presently neither effective vaccines nor drugs available for the prevention or treatment of RSV infections. In this study, we investigated the effect of the plant extract resveratrol as a means of preventing airway inflammation and attenuating RSV-induced AHR. Our data showed that resveratrol reduced RSV lung titers and the number of infiltrating lymphocytes present in bronchoalveolar lavage fluid (BALF) and reduced inflammation. Furthermore, resveratrol attenuated airway responses to methacholine following RSV infection and significantly decreased gamma interferon (IFN-γ) levels in BALF of RSV-infected mice. Data presented in this report demonstrated that resveratrol controlled Toll-like receptor 3 (TLR3) expression, inhibited the TRIF signaling pathway, and induced M2 receptor expression following RSV infection. These data support a role for the use of resveratrol as a means of reducing IFN-γ levels associated with RSV-mediated airway inflammation and AHR, which may be mediated via TLR3 signaling.
Addition of enoxaparin to aspirin for the secondary prevention of placental vascular complications in women with severe pre-eclampsia
Authors: Chauleur Céline , Molinari Nicolas , Marès Pierre , Fabbro-Peray Pascale , Quéré Isabelle , Lefrant Jean-Yves , Haddad Bassam , Dauzat Michel , Gris Jean-Christophe 
Published: September 2011

Summarydministration of heparin in the secondary prevention of placental vascular complications is still experimental. In women with a previous severe pre-eclampsia, we investigated the effectiveness of enoxaparin, a low-molecular-weight heparin, in preventing these complications. Between January 2000 and January 2010, 224 women from the NOHA First cohort, with previous severe pre-eclampsia but no foetal loss during their first pregnancy and negative for antiphospholipid antibodies, were randomised to either a prophylactic daily dose of enoxaparin starting from the positive pregnancy test (n=112), or no enoxaparin (n=112). The primary outcome was a composite of at least one of the following: pre-eclampsia, abruptio placentae, birthweight ≤5th percen-tile, or foetal loss after 20 weeks. Enoxaparin was associated with a lower frequency of primary outcome: 8.9% (n=10/112) vs. 25 % (28/112), p=0.004, hazard ratio = 0.32, 95% confidence interval (0.16–0.66), p=0.002. Enoxaparin was safe, with no obvious side-effect, no thrombocytopenia nor major bleeding event excess. This pilot study shows that enoxaparin given early during the second pregnancy decreases the occurrence of placental vascular complications in women with a previous severe pre-eclampsia during their first pregnancy.The pilot randomised controlled NOH-PE trial
Comparison of antithrombotic efficacy between edoxaban, a direct factor Xa inhibitor, and fondaparinux, an indirect factor Xa inhibitor under low and high shear rates
Authors: Tsuji Naoki , Honda Yuko , Kamisato Chikako , Morishima Yoshiyuki , Shibano Toshiro , Fukuda Toshio 
Published: September 2011

SummaryEdoxaban is an oral, direct factor Xa (FXa) inhibitor under late-phase clinical development. This study compared the antithrombotic efficacy of edoxaban with that of an indirect FXa inhibitor, fondaparinux, in in vivo venous and arterial thrombosis models and in ex vivo perfusion chamber thrombosis model under low and high shear rates in rats. Venous and arterial thrombi were induced by platinum wire insertion into the inferior vena cava and by application of FeCl3 to the carotid artery, respectively. The perfusion chamber thrombus was formed by blood perfusion into a collagen-coated capillary at 150 s-1 (low shear rate) and 1,600 s-1 (high shear rate). Effective doses of edoxaban that reduced thrombus formation by 50% (ED50) in venous and arterial thrombosis models were 0.076 and 0.093 mg/kg/h, respectively. In contrast, ED50 of fondaparinux in the arterial thrombosis model (>10 mg/kg/h) was markedly higher compared to ED50 in the venous thrombosis model (0.021 mg/kg/h). In the perfusion chamber thrombosis model, the ratio of ED50 under high shear rate (1.13 mg/kg/h) to that under low shear rate (0.63 mg/kg/h) for edoxaban was 1.9, whereas that for fondaparinux was more than 66. While the efficacy of fondaparinux markedly decreased in arterial thrombosis and in a high-shear state, edoxaban exerted consistent antithrombotic effects regardless of flow conditions. These results suggest that shear rate is a key factor in different antithrombotic effects between edoxaban and fondaparinux.