bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microenvironment alters epigenetic and gene expression profiles in Swarm rat chondrosarcoma tumors
Authors: Hamm Christopher A , Stevens Jeff W , Xie Hehuang , Vanin Elio F , Morcuende Jose A , Abdulkawy Hakeem , Seftor Elisabeth A , Sredni Simone T , Bischof Jared M , Wang Deli , Malchenko Sergey , de Fatima Bonaldo Maria , Casavant Thomas L , Hendrix Mary JC , Soares Marcelo B 
Published: September 2010

AbstractBackgroundChondrosarcomas are malignant cartilage tumors that do not respond to traditional chemotherapy or radiation. The 5-year survival rate of histologic grade III chondrosarcoma is less than 30%. An animal model of chondrosarcoma has been established - namely, the Swarm Rat Chondrosarcoma (SRC) - and shown to resemble the human disease. Previous studies with this model revealed that tumor microenvironment could significantly influence chondrosarcoma malignancy.MethodsTo examine the effect of the microenvironment, SRC tumors were initiated at different transplantation sites. Pyrosequencing assays were utilized to assess the DNA methylation of the tumors, and SAGE libraries were constructed and sequenced to determine the gene expression profiles of the tumors. Based on the gene expression analysis, subsequent functional assays were designed to determine the relevancy of the specific genes in the development and progression of the SRC.ResultsThe site of transplantation had a significant impact on the epigenetic and gene expression profiles of SRC tumors. Our analyses revealed that SRC tumors were hypomethylated compared to control tissue, and that tumors at each transplantation site had a unique expression profile. Subsequent functional analysis of differentially expressed genes, albeit preliminary, provided some insight into the role that thymosin-β4, c-fos, and CTGF may play in chondrosarcoma development and progression.ConclusionThis report describes the first global molecular characterization of the SRC model, and it demonstrates that the tumor microenvironment can induce epigenetic alterations and changes in gene expression in the SRC tumors. We documented changes in gene expression that accompany changes in tumor phenotype, and these gene expression changes provide insight into the pathways that may play a role in the development and progression of chondrosarcoma. Furthermore, specific functional analysis indicates that thymosin-β4 may have a role in chondrosarcoma metastasis.
Relationship between tumor size and disease stage in non-small cell lung cancer
Authors: Yang Fu , Chen Haiquan , Xiang Jiaqing , Zhang Yawei , Zhou Jianhua , Hu Hong , Zhang Jie , Luo Xiaoyang 
Published: September 2010

Abstract

Background
Whether tumor size and stage distribution are correlated remains controversial. The objective is to assess the relationship between tumor size and disease stage distribution in non-small cell lung cancer (NSCLC).

Methods
We conducted a retrospective analysis of 917 cases of NSCLC that were resected in the Cancer Hospital of Fudan University and Shanghai Sixth Hospital between January 2000 and February 2009. Tumor sizes were grouped into five categories: ≤20 mm, 21 to 30 mm, 31 to 50 mm, 51 to 70 mm and ≥71 mm.

Results
Age and tumor size affected stage distribution: patients 60 years or older had a higher percentage of N0M0 disease than patients younger than 60 years (61.67% vs. 44.85%, p < 0.01). The smaller the tumor, the more likely the disease was N0M0 status (p < 0.05). For tumors ≤20 mm in diameter, the proportion of cases with N0M0 status was 70.79%, compared to 58.88% for 21 to 30 mm, 48.03% for 31 to 50 mm, 47.55% for 51 to 70 mm, 33.33% for ≥71 mm. The mean (± SD) tumor size of cases with N0M0 status was 37.17 ± 21.34 mm, compared to 45.75 ± 23.19 mm for cases with other status.

Conclusions
There is a statistically significant relationship between tumor size and distribution of disease stage of primary NSCLC tumors: the smaller the tumor, the more likely the disease is N0M0 status.
Sprouty1, a new target of the angiostatic agent 16K prolactin, negatively regulates angiogenesis
Authors: Sabatel Céline , Cornet Anne M , Tabruyn Sébastien P , Malvaux Ludovic , Castermans Karolien , Martial Joseph A , Struman Ingrid 
Published: September 2010

Abstract

Background
Disorganized angiogenesis is associated with several pathologies, including cancer. The identification of new genes that control tumor neovascularization can provide novel insights for future anti-cancer therapies. Sprouty1 (SPRY1), an inhibitor of the MAPK pathway, might be one of these new genes. We identified SPRY1 by comparing the transcriptomes of untreated endothelial cells with those of endothelial cells treated by the angiostatic agent 16 K prolactin (16 K hPRL). In the present study, we aimed to explore the potential function of SPRY1 in angiogenesis.

Results
We confirmed 16 K hPRL induced up-regulation of SPRY1 in primary endothelial cells. In addition, we demonstrated the positive SPRY1 regulation in a chimeric mouse model of human colon carcinoma in which 16 K hPRL treatment was shown to delay tumor growth. Expression profiling by qRT-PCR with species-specific primers revealed that induction of SPRY1 expression by 16 K hPRL occurs only in the (murine) endothelial compartment and not in the (human) tumor compartment. The regulation of SPRY1 expression was NF-κB dependent. Partial SPRY1 knockdown by RNA interference protected endothelial cells from apoptosis as well as increased endothelial cell proliferation, migration, capillary network formation, and adhesion to extracellular matrix proteins. SPRY1 knockdown was also shown to affect the expression of cyclinD1 and p21 both involved in cell-cycle regulation. These findings are discussed in relation to the role of SPRY1 as an inhibitor of ERK/MAPK signaling and to a possible explanation of its effect on cell proliferation.

Conclusions
Taken together, these results suggest that SPRY1 is an endogenous angiogenesis inhibitor.
Development of Benzylic-Substituted Ligands for Asymmetric Catalysis
Authors: Ding Chang-Hua , Hou Xue-Long 
Published: September 2010

Abstract
This account demonstrates our recent work in the design and synthesis of benzylic-substituted chiral ligands and their applications in metal-catalyzed asymmetric catalysis. High catalytic activity and asymmetric induction have been shown in many enantioselective transformations. The relationship of flexibility and rigidity as well as the impact of substituents at the benzylic position of ligands on the reactions has been revealed. The switch of enantioselectivity in the palladium-catalyzed Heck reaction was observed using ligands with different substituents at the benzylic position of the ligands. Based upon the benzylic-substituted framework, palladacycles were synthesized, which served as a real catalyst in the ring opening reaction of oxabicyclic alkenes with organozinc halides. Highly efficient kinetic resolution of 2-aryl-1-hydroxy-1,2-dihydronaphthalenes has also been developed via dehydration using benzylic-substituted chiral palladacycles.
Liquid–liquid extraction of Nd(III) and Eu(III) using nalidixic acid in dichloromethane
Authors: Ali Akbar , Arafat Abbasi Yasar , Haleem Khan Muhammad , Mufazzal Saeed Muhammad 
Published: September 2010

AbstractThe extraction of Nd(III) and Eu(III) as representatives of rare earth elements with 0.1 mol dm−3nalidixic acid (HNA) in dichloromethane was studied from aqueous solutions of pH 1–8. The quantitative extraction of these metals was observed at pH 6.5. Various parameters affecting their extraction were optimized. The composition of the extracted adduct was determined by slope analysis method that came out to be [(M(NA)2)+(CH3COO)−] where M represents Nd(III) or Eu(III). The effect of various anions and cations on the extraction of these metal ions was also studied. Among the anions, sulfate and phosphate interfered significantly, whereas Fe(III), Cu(II) and Al(III) reduced the extraction.
A New Pressure/Rate-Deconvolution Algorithm To Analyze Wireline-Formation-Tester and Well-Test Data
Authors: Pimonov Evgeny , Ayan Cosan , Onur Mustafa , Kuchuk Fikri 
Published: September 2010

Summary
Reconstructing constant-rate drawdown-pressure response and its logarithmic time (or Bourdet) derivative by deconvolution from multirate pressure-transient data is very important for wellbore-/ reservoir-system identification and interpretation. In recent years, the use of pressure/rate deconvolution has increased considerably because of significant improvement of the algorithms. In this paper, we present a new deconvolution algorithm based on a weighted Euclidean norm in the Tikhonov (1963) regularized objective function so that one can assign weights to individual pressure- and rate-measurement points, and, thus, define different error estimates for different sections of the data. Incorporating such features into the deconvolution algorithm is very useful to mitigate the effects of unreliable pressure and rate measurements and the sections of the data not obviously consistent with the wellbore/reservoir model.
We present two applications of the new algorithm using real field pressure/rate transient data sets. In addition to conventional drillstem-test (DST) well-test data, we apply the algorithm to wireline formation-tester (WFT) pressure transients, which are usually also referred to as interval pressure-transient tests (IPTTs). The results show that the new deconvolution algorithm presented in this paper is useful in interpreting pressure/rate transient data from both formation and well tests.
A Model To Calculate the Theoretical Critical Flow Rate Through Venturi Gas Lift Valves
Authors: Almeida Alcino R. 
Published: September 2010

Summary
A model to calculate the theoretical critical flow rate of nitrogen (N2) or natural gas through a Venturi gas lift valve is described herein. This new model considers real-gas effects not only in density calculations but also in other thermodynamic properties that are relevant during gas isentropic evolution.
For the properties of N2, the Bennedict, Webb, and Rubin (BWR) equation of state and an accurate correlation for the ideal-gas isobaric heat capacity were used. For natural gas, the Dranchuk and Abou-Kassem equation, which reproduces the well-known Standing and Katz chart, was used, and, for the ideal-gas isobaric heat capacity, it was assumed that the natural gas was a mixture of methane and ethane only, their individual ideal-gas heat capacity being calculated by updated correlations.
To validate the use of the proposed equations of state, a comparison of calculated with experimental or reference data on properties of N2 and natural gas (including pure methane and some relevant mixtures) was performed with very good results for N2 and for natural-gas compositions usual in gas lift operations (dry gas with very small amounts of contaminants). For natural gas with moderate amounts of N2 and carbon dioxide (CO2), accurate results were obtained after correction of critical conditions and of ideal heat capacity. The model was also compared with other theoretical models found in the literature, which use compositional approaches for natural gas, with excellent results. Some experimental results obtained with commercial Venturi valves manufactured in Brazil are also presented.
Pharmacologic Immunomodulation and Cutaneous Malignancy in Rheumatoid Arthritis, Psoriasis, and Psoriatic Arthritis
Authors: KRATHEN MICHAEL S. , GOTTLIEB ALICE B. , MEASE PHILIP J. 
Published: September 2010

Objective.It is unclear if skin cancer risk is affected by the use of immunomodulatory medications in rheumatoid arthritis (RA), psoriasis, and psoriatic arthritis (PsA). The purpose of this study is to evaluate and summarize the available data pertinent to this question.Methods.The English language literature on PubMed was searched with a combination of phrases, including “malignancy,” “skin cancer,” “squamous cell carcinoma,” “basal cell carcinoma,” “melanoma,” “psoriasis,” “psoriatic arthritis,” and “rheumatoid arthritis” in addition to the generic names of a variety of common immunomodulatory drugs. Relevant articles were identified and data were extracted.Results.In total, 2218 potentially relevant articles were identified through the search process. After further screening, 20 articles relevant to RA were included. An additional 19 articles relevant to either psoriasis or PsA were included as well. RA may be a risk factor for the development of cutaneous malignancy. Treatment with tumor necrosis factor inhibitors increases the rates of non-melanoma skin cancer (NMSC) in RA and psoriasis. This risk doubles when combination methotrexate therapy is used in RA. Methotrexate may increase the risk of malignant melanoma in patients with RA and the risk of NMSC in psoriasis. Cyclosporine and prior phototherapy significantly increase the risk of NMSC.Conclusion.RA may potentiate the risk of cutaneous malignancy and therefore dermatologic screening in this population should be considered. The use of immunomodulatory therapy in RA, psoriasis, and PsA may further increase the risk of cutaneous malignancy and therefore dermatologic screening examinations are warranted in these groups. More careful recording of skin cancer development during clinical trials and cohort studies is necessary to further delineate the risks of immunomodulatory therapy.
Emerging variants, unique phenotypes, and transcriptomic signatures: an integrated study of COASY‐associated diseases
Authors: Cavestro Chiara , Morra Francesca , Legati Andrea , D'Amato Marco , Nasca Alessia , Iuso Arcangela , Lubarr Naomi , Morrison Jennifer L. , Wheeler Patricia G. , Serra‐Juhé Clara , Rodríguez‐Santiago Benjamín , Turón‐Viñas Eulalia , Prouteau Clement , Barth Magalie , Hayflick Susan J. , Ghezzi Daniele , Tiranti Valeria , Di Meo Ivano 
Published: May 2024

AbstractObjectiveCOASY, the gene encoding the bifunctional enzyme CoA synthase, which catalyzes the last two reactions of cellular de novo coenzyme A (CoA) biosynthesis, has been linked to two exceedingly rare autosomal recessive disorders, such as COASY protein‐associated neurodegeneration (CoPAN), a form of neurodegeneration with brain iron accumulation (NBIA), and pontocerebellar hypoplasia type 12 (PCH12). We aimed to expand the phenotypic spectrum and gain insights into the pathogenesis of COASY‐related disorders.MethodsPatients were identified through targeted or exome sequencing. To unravel the molecular mechanisms of disease, RNA sequencing, bioenergetic analysis, and quantification of critical proteins were performed on fibroblasts.ResultsWe identified five new individuals harboring novel COASY variants. While one case exhibited classical CoPAN features, the others displayed atypical symptoms such as deafness, language and autism spectrum disorders, brain atrophy, and microcephaly. All patients experienced epilepsy, highlighting its potential frequency in COASY‐related disorders. Fibroblast transcriptomic profiling unveiled dysregulated expression in genes associated with mitochondrial respiration, responses to oxidative stress, transmembrane transport, various cellular signaling pathways, and protein translation, modification, and trafficking. Bioenergetic analysis revealed impaired mitochondrial oxygen consumption in COASY fibroblasts. Despite comparable total CoA levels to control cells, the amounts of mitochondrial 4′‐phosphopantetheinylated proteins were significantly reduced in COASY patients.InterpretationThese results not only extend the clinical phenotype associated with COASY variants but also suggest a continuum between CoPAN and PCH12. The intricate interplay of altered cellular processes and signaling pathways provides valuable insights for further research into the pathogenesis of COASY‐associated diseases.
Influence of a Preageing Treatment on the Cluster Formation and the Further Ageing Route in Al–Mg–Si Extrusion Alloys
Authors: Ortner Patrick , Schiffl Andreas , Höppel Heinz Werner 
Published: May 2024

Herein, the influence of one‐ and two‐step preageing treatments prior to the artificial ageing step to peak age condition for three different 6xxx alloys, from low to high alloy content, is investigated. Analysis of the preaged conditions using atom probe tomography (APT) reveals a broad distribution of Mg and Si clusters, with some instances of Guinier‐Preston I zones present. Additionally, the high alloyed system exhibits a growth of precipitates toward a needle‐like shaped. These clusters formed during the preageing treatment serve as nucleation sites in the subsequent artificial ageing treatment, leading to an increase in hardness. Microstructural analysis conducted using transmission electron microscopy and APT demonstrates a reduction in the average precipitate length and a more narrow size distribution for T6 condition. This indicates that the preageing treatment results in a decreased portion of already overaged precipitates compared to the direct aged condition. Consequently, the more narrow size distribution of the precipitates leads to more effective hardening of the material, as a higher portion of precipitates is at or close to their optimum size. The investigations highlight the significant influence and positive effect of a preageing treatment at lower temperatures on the subsequent artificial ageing treatment.
Adaptive Interfacial Materials and Implants for Visual Restoration
Authors: Zhu Xule , Wang Fang , Zhao Qilong , Du Xuemin 
Published: May 2024

AbstractBlindness is a globally critical challenge for public health, affecting more than 43 million individuals worldwide. Retinal degenerative diseases including retinitis pigmentosa and age‐related macular degeneration, involving degeneration of retinal pigment epithelium (RPE) and thereby the dysfunction of retina, are primary causes of blindness. For treating these retinal degeneration diseases, emerging interfacial materials and implants that can adapt to nerve tissues and stimulate residual neurons within the visual pathway have shown particular promise, where some issued products are approved for commercialization. Given the attractive opportunities and challenges of the interfacial materials and implants for visual restoration, a comprehensive and state‐of‐the‐art review that provides insight into their design principle and biological performances will be urgently required yet missing. In this review, the latest progress of these adaptive interfacial materials and implants for visual restoration will be summarized, and their challenges and opportunities for clinical use will be further discussed.
Boron Nitride Nanosheets: Thickness‐Related Properties and Applications
Authors: Cai Qiran , Li Lu Hua , Mateti Srikanth , Bhattacharjee Amrito , Fan Ye , Huang Shaoming , Chen Ying Ian 
Published: May 2024

AbstractOwing to its exceptional properties and wide‐ranging potential applications from aerospace to medicine, hexagonal boron nitride (h‐BN) has garnered considerable attention over the past decades. Boron nitride nanosheets (BNNSs), atomically thin h‐BN, not only inherit most of the outstanding properties of h‐BN but also exhibit superior characteristics compared to their bulk counterpart due to their reduced thickness, such as special adsorption behaviors and enhanced thermal conductivity. Furthermore, BNNSs display distinct thickness‐dependent properties from graphene and other 2D materials, such as unique mechanical response under indentation. This feature article provides an overview of the thickness‐related special properties of BNNSs, primarily derived from mechanically exfoliated h‐BN single crystals. These properties span various domains, including Raman signatures, molecule adsorption‐induced conformational changes, mechanical properties, thermal conductivity, and thermal expansion coefficients. Moreover, the feature article explores the underlying mechanisms governing these atomic‐scale thickness effects. Leveraging their unique properties, the feature article investigates diverse applications of BNNSs, encompassing surface‐enhanced Raman spectroscopy, metal‐enhanced fluorescence, and isotropic thermal management.
A Large‐Scale Fabrication of Flexible, Ultrathin, and Robust Solid Electrolyte for Solid‐State Lithium‐Sulfur Batteries
Authors: Nie Lu , Zhu Jinling , Wu Xiaoyan , Zhang Mengtian , Xiao Xiao , Gao Runhua , Wu Xinru , Zhu Yanfei , Chen Shaojie , Han Zhiyuan , Yu Yi , Wang Shaogang , Ling Shengjie , Zhou Guangmin 
Published: May 2024

AbstractAll‐solid‐state lithium metal batteries (ASSLMBs) are considered as the most promising candidates for the next‐generation high‐safety batteries. To achieve high energy density in ASSLMBs, it is essential that the solid‐state electrolytes (SSEs) are lightweight, thin, and possess superior electrochemical stability. In this study, a feasible and scalable fabrication approach to construct 3D supporting skeleton using an electro‐blown spinning technique is proposed. This skeleton not only enhances the mechanical strength but also hinders the migration of Li‐salt anions, improving the lithium‐ion transference number of the SSE. This provides a homogeneous distribution of Li‐ion flux and local current density, promoting uniform Li deposition. As a result, based on the mechanically robust and thin SSEs, the Li symmetric cells show outstanding Li plating/stripping reversibility. Besides, a stable interface contact between SSE and Li anode has been established with the formation of an F‐enriched solid electrolyte interface layer. The solid‐state Li|sulfurized polyacrylonitrile (Li|SPAN) cell achieves a capacity retention ratio of 94.0% after 350 cycles at 0.5 C. Also, the high‐voltage Li|LCO cell shows a capacity retention of 92.4% at 0.5 C after 500 cycles. This fabrication approach for SSEs is applicable for commercially large‐scale production and application in high‐energy‐density and high‐safety ASSLMBs.
In Situ Preparation of Superconducting Infinite‐Layer Nickelate Thin Films with Atomically Flat Surface
Authors: Sun Wenjie , Wang Zhichao , Hao Bo , Yan Shengjun , Sun Haoying , Gu Zhengbin , Deng Yu , Nie Yuefeng 
Published: May 2024

AbstractSince their discovery, the infinite‐layer nickelates have been regarded as an appealing system for gaining deeper insights into high‐temperature superconductivity (HTSC). However, the synthesis of superconducting samples has been proven to be challenging. Here, an ultrahigh vacuum (UHV) reduction method is developed using atomic hydrogen as a reducing agent and is applied in the lanthanum nickelate system. The reduction parameters, including the reduction temperature (TR) and hydrogen pressure (PH), are systematically explored. It is found that the reduction window for achieving superconducting transition is quite wide, reaching nearly 80°C in TR and three orders of magnitude in PH when the reduction time is set to 30 min. And there exists an optimal PH for achieving the highest Tc if both TR and reduction time are fixed. More prominently, as confirmed by atomic force microscopy and scanning transmission electron microscopy, the atomically flat surface can be preserved during the reduction process, providing advantages over the CaH2 method for surface‐sensitive experiments.