bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cutting Edge: IL-10–Mediated Tristetraprolin Induction Is Part of a Feedback Loop That Controls Macrophage STAT3 Activation and Cytokine Production
Authors: Gaba Anthony , Grivennikov Sergei I. , Do Mahn Vu , Stumpo Deborah J. , Blackshear Perry J. , Karin Michael 
Published: August 2012

Abstract
In activated macrophages, the anti-inflammatory cytokine IL-10 inhibits expression of molecules that propagate inflammation in a manner that depends on transcription factor STAT3. Expression of IL-10 is regulated posttranscriptionally by the RNA-binding protein tristetraprolin (TTP), which destabilizes IL-10 mRNA in activated macrophages. Using LPS-activated bone marrow-derived murine macrophages, we demonstrate that TTP is a negative regulator of the IL-10/STAT3 anti-inflammatory response. LPS-stimulated TTP-deficient macrophages overproduced IL-10, contained increased amounts of activated STAT3, and showed reduced expression of inflammatory cytokines, including cytokines encoded by TTP target mRNAs. Thus, in LPS-stimulated TTP-deficient macrophages, increased IL-10/STAT3 anti-inflammatory control was dominant over the mRNA stabilization of specific TTP targets. The TTP gene promoter contains a conserved STAT3 binding site, and IL-10 induces STAT3 recruitment to this site. Correspondingly, STAT3 was required for efficient IL-10–induced TTP expression. Hence, by inducing TTP expression, STAT3 activates a negative regulatory loop that controls the IL-10/STAT3 anti-inflammatory response.
Shaping of Human Germline IgH Repertoires Revealed by Deep Sequencing
Authors: Larimore Kevin , McCormick Michael W. , Robins Harlan S. , Greenberg Philip D. 
Published: August 2012

Abstract
To understand better how selection processes balance the benefits of Ig repertoire diversity with the risks of autoreactivity and nonfunctionality of highly variable IgH CDR3s, we collected millions of rearranged germline IgH CDR3 sequences by deep sequencing of DNA from mature human naive B cells purified from four individuals and analyzed the data with computational methods. Long HCDR3 regions, often components of HIV-neutralizing Abs, appear to derive not only from incorporation of long D genes and insertion of large N regions but also by usage of multiple D gene segments in tandem. However, comparison of productive and out-of-frame IgH rearrangements revealed a selection bias against long HCDR3 loops, suggesting these may be disproportionately either poorly functional or autoreactive. Our data suggest that developmental selection removes HCDR3 loops containing patches of hydrophobicity, which are commonly found in some auto-antibodies, and at least 69% of the initial productive IgH rearrangements are removed from the repertoire during B cell development. Additionally, we have demonstrated the potential utility of this new technology for vaccine development with the identification in all four individuals of related candidate germline IgH precursors of the HIV-neutralizing Ab 4E10.
There is Beauty Here, Too:
Authors: Poulakos John , Crick Nathan 
Published: August 2012

Abstract
In Aristotle's biological treatise, On the Parts of Animals, one finds a rare and unexpected burst of rhetorical eloquence. While justifying the study of “less valued animals,” he erupts into praise for the study of all natural phenomena and condemns the small-mindedness of those who trivialize its worth. Without equal in Aristotle's remaining works for its rhetorical quality, it reveals the otherwise coolheaded researcher as a passionate seeker of truth and an unabashed lover of natural beauty. For Aristotle, rhetoric not only discloses the truth (aletheia) of appearances by refuting counterarguments and defending one's claims within agonistic forums; rhetoric also defends and advances whole fields of study on the promise on wonder (thaumazein). By examining Aristotle's example in practice, this article seeks to elucidate a notion of the rhetoric for inquiry that calls lovers of wisdom to the empirical study of nature.
Palladium‐Catalyzed CC Coupling of Aryl Halides with Isocyanides: An Alternative Method for the Stereoselective Synthesis of (3E)‐(Imino)isoindolin‐1‐ones and (3E)‐(Imino)thiaisoindoline 1,1‐Dioxides
Authors: Liu Bifu , Li Yibiao , Jiang Huanfeng , Yin Meizhou , Huang Huawen 
Published: August 2012

AbstractA palladium‐catalyzed, one‐pot cyclization reaction to construct (3E)‐(imino)isoindolin‐1‐ones and (3E)‐(imino)thiaisoindoline 1,1‐dioxides by introducing ortho‐reactive functional groups on aryl halides is reported. Under optimal conditions, the cyclization reaction afforded the corresponding products in good to excellent yields (up to 93%) with high stereoselectivity. Notably, this transformation successfully extends its application for the synthesis of phenanthridines and dibenzooxazepines. This new synthetic protocol not only extends the application platform for palladium‐catalyzed CC coupling of aryl halides with isocyanides, but also opens atom‐economic and step‐economic synthetic routes for nitrogen‐containing heterocyclic compounds with wide functional group compatibility.
Mild and High‐Yielding Molybdenum(VI) Dichloride Dioxide‐Catalyzed Formation of Mono‐, Di‐, Tri‐, and Tetracarbamates from Alcohols and Aromatic or Aliphatic Isocyanates
Authors: Stock Christian , Brückner Reinhard 
Published: August 2012

AbstractBoth molybdenum(VI) dichloride dioxide (MoO2Cl2) and its dimethylformamide (DMF) complex catalyze the addition of alcohols to isocyanates giving carbamates. Most additions proceeded to completion at room temperature within 20 min using as little as 0.1 mol% of the catalyst when working on a 1‐mmol scale or just 100 ppm working on a 20‐mmol scale. Sterically encumbered substrates reacted to completion when 1 mol% of the catalyst was employed. Diols, triols, and tetraols reacted with monoisocyanates likewise, as did monofunctional alcohols and diisocyanates. These pairings furnished di‐, tri‐, tetra‐, and dicarbamates, respectively. Reactants, which were poorly soluble in CH2Cl2 at room temperature required elevating the temperature and possibly choosing a higher‐boiling solvent (ClCH2CH2Cl, DMF) as well. Additions of diols to diisocyanates were feasible, too, giving polycarbamates as we presume.
Growth charts for 22q11 deletion syndrome
Authors: Tarquinio Daniel C. , Jones Marilyn C. , Jones Kenneth Lyons , Bird Lynne M. 
Published: August 2012

AbstractThe purpose of this work was to create growth curves specific to the 22q11.2 deletion syndrome. Growth parameters on 188 patients (86 females, 102 males) followed by a group of three dysmorphologists were collected by retrospective chart review. Growth charts for body mass, length/height, and head circumference were generated using a semi‐parametric model with goodness‐of‐fit tests. The resulting charts show that between 25 and 50% of children with 22q11.2 deletion syndrome fall below the 2nd centile for the normal population for all growth parameters. Establishing norms of growth for 22q11.2 deletion syndrome allows the clinician to identify and investigate those children who deviate substantially from the growth profile of this condition. © 2012 Wiley Periodicals, Inc.
IMPAD1 mutations in two Catel‐Manzke like patients
Authors: Nizon Mathilde , Alanay Yasemin , Tuysuz Beyhan , Kiper Pelin Ozlem Simsek , Geneviève David , Sillence David , Huber Celine , Munnich Arnold , Cormier‐Daire Prof. Valérie 
Published: August 2012

AbstractCatel–Manzke syndrome is characterized by hyperphalangism with bilateral deviation of the index fingers and micrognathia with or without cleft palate. Some atypical patients present with additional malformations. No molecular basis is yet available. Most patients have an unremarkable family history but autosomal recessive inheritance has been recently suggested in a consanguineous family with recurrence in sibs. Catel–Manzke syndrome has overlapping features with Desbuquois dysplasia type 1 due to CANT1 (calcium‐activated nucleotidase 1) mutations and also with “chondrodysplasia with joint dislocations, gPAPP type” due to IMPAD1 (Inositol Monophosphatase Domain containing 1) mutations recently reported in four patients, all characterized by short stature, joint dislocations, brachydactyly and cleft palate. The aim of our study was to screen CANT1 and IMPAD1 in Catel–Manzke patients. Three patients were diagnosed as classical Catel–Manzke syndrome and two as Catel–Manzke like patients, based on the presence of additional features. We identified two homozygous loss‐of‐function IMPAD1 mutations in the two Catel–Manzke like patients (p.Arg187X and p.Ser108ArgfsX48). The phenotype was characterized by severe growth retardation with short and abnormal extremities, cleft palate with micrognathia and knee hyperlaxity. Radiographs of hands and feet revealed numerous accessory bones with abnormally shaped phalanges and carpal synostosis. Based on this report, we concluded that IMPAD1 should be screened for patients with Catel–Manzke and additional features. © 2012 Wiley Periodicals, Inc.
IRF6 is a risk factor for nonsyndromic cleft lip in the Brazilian population
Authors: Brito Luciano A. , Bassi Camila F.S. , Masotti Cibele , Malcher Carolina , Rocha Kátia M. , Schlesinger David , Bueno Daniela F. , Cruz Lucas A. , Barbara Ligia K. , Bertola Débora R. , Meyer Diogo , Franco Diogo , Alonso Nivaldo , Passos‐Bueno Maria Rita 
Published: August 2012

AbstractNonsyndromic cleft lip with or without cleft palate (NSCL/P) is a complex disorder with a worldwide incidence estimated at 1:700. Among the putative susceptibility loci, theIRF6gene and a region at 8q24.21 have been corroborated in different populations. To test the role ofIRF6in NSCL/P predisposition in the Brazilian population, we conducted a structured association study with the SNPs rs642961 and rs590223, respectively, located at 5′ and 3′ of theIRF6gene and not in strong linkage disequilibrium (LD), in patients from five different Brazilian locations. We also evaluated the effect of these SNPs inIRF6expression in mesenchymal stem cells (MSC). We observed association between rs642961 and cleft lip only (CLO) (P = 0.009; odds ratio (OR) for AA genotype = 1.83 [95% Confidence interval (CI), 0.64–5.31]; OR for AG genotype = 1.72 [95% CI, 1.03–2.84]). This association seems to be driven by the affected patients from Barbalha, a location which presents the highest heritability estimate (H2 = 0.85), and the A allele at rs642961 is acting through a dominant model. No association was detected for the SNP rs590223. We did not find any correlation between expression levels and genotypes of the two loci, and it is possible that these SNPs have a functional role in some specific period of embryogenesis. © 2012 Wiley Periodicals, Inc.
Selective Synthesis and Structural Confirmation of 1,3‐Oxazines and Schiff Bases with Controllable Substitution Patterns
Authors: Sundaramoorthy Karthikeyan Natesan , Ramachandran Gunasekar , Rathore Ravindranath S , Iyer Sathiyanarayanan Kulathu 
Published: August 2012

AbstractSelective synthesis of 2,4‐diaryl‐2,3‐dihydro‐3H‐naphtho[1,2‐e]‐1,3‐oxazines and Schiff bases ((E)‐[1‐arylmethyl(benzylideneamino)]naphth‐2‐ols) that contain two identical or two different aryl substituents was achieved through one‐pot synthesis with ammonium acetate. From the proposed mechanism the dual role of ammonium acetate was identified. The synthesized products were characterized by 1H and 13C NMR spectroscopy, as well as FTIR and mass spectrometry. Crystal structures of the 1,3‐oxazines and Schiff base derivatives were examined. A notable feature is the conspicuous absence of strong donors (NH/OH) in any intermolecular hydrogen‐bonding scheme. Instead, packing is characterized by weak intermolecular CH⋅⋅⋅O, CH⋅⋅⋅π and CCl⋅⋅⋅π interactions, which lead to dimeric and zig‐zag one‐dimensional molecular patterns. The oxazine moiety of the tricyclic ring system predominantly adopts a half‐chair conformation. The present Mannich procedure is an environmentally benign and cost‐effective method for the preparation of industrially and pharmaceutically useful heterocyclic scaffolds.
Luminescent Ionic Transition‐Metal Complexes for Light‐Emitting Electrochemical Cells
Authors: Costa Rubén D. , Ortí Enrique , Bolink Henk J. , Monti Filippo , Accorsi Gianluca , Armaroli Nicola 
Published: August 2012

AbstractHigher efficiency in the end‐use of energy requires substantial progress in lighting concepts. All the technologies under development are based on solid‐state electroluminescent materials and belong to the general area of solid‐state lighting (SSL). The two main technologies being developed in SSL are light‐emitting diodes (LEDs) and organic light‐emitting diodes (OLEDs), but in recent years, light‐emitting electrochemical cells (LECs) have emerged as an alternative option. The luminescent materials in LECs are either luminescent polymers together with ionic salts or ionic species, such as ionic transition‐metal complexes (iTMCs). Cyclometalated complexes of IrIII are by far the most utilized class of iTMCs in LECs. Herein, we show how these complexes can be prepared and discuss their unique electronic, photophysical, and photochemical properties. Finally, the progress in the performance of iTMCs based LECs, in terms of turn‐on time, stability, efficiency, and color is presented.
CH Bond Functionalization: Emerging Synthetic Tools for Natural Products and Pharmaceuticals
Authors: Yamaguchi Junichiro , Yamaguchi Atsushi D. , Itami Kenichiro 
Published: August 2012

AbstractThe direct functionalization of CH bonds in organic compounds has recently emerged as a powerful and ideal method for the formation of carbon–carbon and carbon–heteroatom bonds. This Review provides an overview of CH bond functionalization strategies for the rapid synthesis of biologically active compounds such as natural products and pharmaceutical targets.
Microstructure and properties of branched polyethylene: Application of a three‐phase structural model
Authors: Martín S. , Expósito M. T. , Vega J. F. , Martínez‐Salazar J. 
Published: August 2012

AbstractA combined study of microstructure and mechanical properties for a series of single‐site metallocene ethylene/1‐hexene copolymers is presented. The analysis of the results focuses on the effect of branching, which in turn modulates crystallinity and density, on the values of the elastic modulus obtained in tensile‐deformation experiments. An extensive literature review is done in order to compare our own results with previous studies. The typical variation found in the elastic modulus is discussed in terms of the existence of a rigid amorphous phase. This rigid phase controls the macroscopic mechanical behavior of the materials, giving rise to an increase of the elastic modulus as the crystallinity does. Mechanical coupling models for heterogeneous systems are applied in order to describe the experimental results of the elastic modulus as a function of the variation of three different phase fractions, i.e., crystalline, rigid amorphous (or interfacial), and mobile amorphous. The phase fraction values obtained from the analysis of the mechanical properties are in qualitative agreement to those found experimentally in our group by Raman infrared spectroscopy and from the literature by positron annihilation lifetime spectroscopy. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Poly(lactic acid)/halloysite nanotubes nanocomposites: Structure, thermal, and mechanical properties as a function of halloysite treatment
Authors: Prashantha Kalappa , Lecouvet Benoît , Sclavons Michel , Lacrampe Marie France , Krawczak Patricia 
Published: August 2012

AbstractPoly(lactic acid) (PLA)/halloysite nanotubes (HNT) nanocomposites were prepared by melt extrusion using a masterbatch dilution process. Effect of addition of both unmodified halloysites (HNT) and quaternary ammonium salt treated halloysites (m‐HNT) was investigated at different nanofiller contents. A homogeneous distribution/dispersion of halloysites in the PLA matrix is obtained for both unmodified and modified nanotubes within the studied composition range. The nucleating effect of halloysites, resulting in a limited increase of degree of crystallinity, is more pronounced in the case of m‐HNT. Besides, the rigidity, tensile, flexural, and impact resistances of PLA significantly increase on addition of halloysites, the property improvement being higher for m‐HNT than for HNT. Interestingly, there is no significant embrittlement (almost constant elongation at break). Based on micromechanical models, this superior reinforcement efficiency of m‐HNT was ascribed to the better interfacial compatibility induced by the modification of the nanotube surface. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Modification and characterization of polyacrylonitrile fiber by chelating ligand for preconcentration and determination of neodymium ion in biological and environmental samples
Authors: Sadeghi Hayedeh Bagheri , Panahi Homayon Ahmad , Abdouss Majid , Esmaiilpour Behnaz , Nezhati Mahshid Nikpour , Moniri Elham , Azizi Zahra 
Published: August 2012

AbstractIn this study a chelating fiber containing iminodiacetic acid was prepared via the stepwise modification of acrylic fibers, polyacrylonitrile. The resulting modified fiber has been characterized by Fourier transform infrared spectroscopy, elemental analysis, thermogravimetric analysis, and scanning electron microscopy. Then polyacrylonitrile‐ iminodiacetic acid was applied for preconcentration and determination of trace Nd(III) ion from human biological fluid and environmental water samples with inductively coupled plasma – atomic emission spectroscopy. The optimum pH value for sorption of the metal ion was 6. The sorption capacity of functionalized resin is 8.9 mg g−1. A recovery of 100% was obtained with preconcentration factor 10 for the Nd(III) with 0.5 M nitric acid as eluting agent. The profile of Nd(III) uptake on this sorbent reflects good accessibility of the chelating sites in the modified polyacrylonitrile. Scatchard analysis revealed that the homogeneous binding sites were formed in the polymers. The equilibrium adsorption data of Nd(III) on modified fiber were analyzed by Langmuir models. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Conductive performances of elastomeric electrolytes based on maleic anhydride‐g‐poly(1, 2‐butadiene) grafting polyethylene glycol 400 and LiClO4
Authors: Wang Jinwei , Zhao Ge 
Published: August 2012

AbstractElastomeric electrolyte films consisting of maleic anhydride‐g‐poly (1, 2‐butadiene) (MAPB) grafting polyethylene glycol 400 (PEG400) as matrix and LiClO4 as alkali salt at the mole ratio of [O]: [Li+] at 6, 8, and 10, respectively are prepared. An equivalent circuit is proposed based on the electronic impedance spectroscopy plots by using the metal/electrolyte film/metal model, the simulant plots are found matched well with the tested plots, and the conductivity variations with salt concentrations and temperatures are calculated accordingly. The film with the mole ratio of [O]: [Li+] at 8:1 exhibits higher conductivity at room temperature, due to its relatively stronger dissociation of oxygen atom to LiClO4 as supported by the FTIR spectroscopy. Their conductivity dependent on temperatures obeys the Arrhenius equation from 303 to 363 K, while it keeps almost unchanged at higher temperatures; these changes are found associate with the strong complexation of oxygen atom with Li+ and the state transference of MAPB elastomer as observed by the DSC analysis. Besides, these films are still thermally stable at around 200°C as observed by the TGA analysis and may be promising solid polymer electrolytes with high stability. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Safety and performance of the spider™ patent foramen ovale occluder
Authors: Edwards‐Lehr Tina , Franke Jennifer , Bertog Stefan C. , Bäcker Christina , Wunderlich Nina , Hofmann Ilona , Wilson Neil , Piechaud Jean‐François , Sievert Horst 
Published: August 2012

ObjectivesThe aim of this clinical trial was to assess safety, efficacy, and technical handling of a novel ceramic‐coated double‐disc patent foramen ovale (PFO) occluder.BackgroundThough percutaneous PFO closure is performed with increasing frequency worldwide, certain risks such as thrombus formation and incomplete endothelialization remain. The Spider™ PFO occluder was designed to minimize these risks.MethodsThe Spider™ PFO occluder is a self‐expandable double‐disc device with a ceramic coated nitinol wire mesh and an integrated expanded polytetrafluoroethylene (ePTFE) membrane on the right atrial side and ceramic coated nitinol anchors and ePTFE patch on the left atrial side. Study patient assessments were conducted at baseline, periprocedure, and discharge and at 1‐, 6‐ and 12‐month follow‐up.ResultsFifty‐one patients (mean age 52 ± 14 years; 63% male) were enrolled in the prospective, multicenter clinical trial. Implantation was successful in all patients. Mean procedural time was 30.0 ± 8.6 min. No periprocedural or in‐hospital complications occurred. Four patients (8%) had paroxysmal atrial fibrillation (AF) at 1‐month follow‐up. Otherwise, no procedure or device related adverse events occurred. Importantly, there were no recurrent embolic events. At 1‐month follow‐up 63% (32/51) of patients had no residual shunt with contrast transesophageal echocardiography during Valsalva maneuver. Of all patients who underwent 6‐month echo follow‐up to date, 78% (39/50) had no residual shunt during Valsalva maneuver.ConclusionInitial results with the novel Spider™ PFO occluder show that the device is safe and easy to use for percutaneous closure of PFO. The overall complication rate was low. The rate of AF needs further investigation. © 2012 Wiley Periodicals, Inc.
Feasibility and utility of pre‐procedure ultrasound imaging of the arm to facilitate transradial coronary diagnostic and interventional procedures (PRIMAFACIE‐TRI)
Authors: Kumar Chugh Sanjay , Chugh Sunita , Chugh Yashasvi , Rao Sunil V. 
Published: August 2012

ObjectivesTo assess feasibility and utility of imaging of both arms using ultrasound to facilitate transradial (TR) and transulnar (TU) coronary angiograms (CA) and intervention.BackgroundDespite well recognized advantages, transradial approach (TRA) has challenges that reduce procedural success including small arterial size, anatomical variations, and anomalies of radial artery (RA). The utility of routine pre‐procedural ultrasound of the arm arteries (PPUAA) in facilitating TRA has not been previously studied.MethodsTo determine the role of PPUAA, we performed a single center registry of consecutive patients undergoing diagnostic and interventional procedures between 2006 and 2011. All patients underwent PPUAA of the right and left radial, ulnar (UA), as well as the brachial arteries (BA) in the antecubital fossa using a linear probe. End‐points assessed included the incidence and correlates of arterial sizes, vascular anomalies, procedure success, and fluoroscopy as well as ultrasound assessment times. RA occlusion rates were studied in the last 10 months of the study period.ResultsComplete data on radial (mean 1.9 mm (male);1.7 mm (female)) and ulnar artery size (mean 1.8 mm (male); 1.6 mm (female)) and data on brachial branching anatomy were available in 2,344 patients; 1,872 of whom underwent a TR or TU procedure. The mean time to perform bilateral PPUAA was 6.4 min ± 1.8 min. The incidence of arterial abnormalities was 9.8% in PPUAA. Procedure success was 98.7% for CA and 97.5% for percutaneous coronary intervention. Outcomes were better in this cohort compared with remaining 3,781 patients in whom PPUAA data were not available.ConclusionThis single center prospective registry shows that PPUAA is feasible, requires minimum time, and provides anatomical information that may improve procedure success while reducing patient discomfort, arterial spasm, and fluoroscopy time. These findings should be confirmed in a randomized trial. © 2013 Wiley Periodicals, Inc.