bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kepribadian Ihsan dan Faktor yang mempengaruhi Kualitas Laporan Keuangan Pemerintah Daerah
Authors: Ariani Nadia Elsa , Arif Afendi  
Published: February 2025

Financial statements are made as a form of accountability for regional financial management to the general public and also the parties concerned. The preparation of these financial statements is a form of accountability, namely as reporting in realizing organizational goals. Good financial statements certainly meet the normative requirements of financial statement quality. The financial statements of the Local Government of Jepara Regency have received an unqualified opinion eleven times in a row. However, the opinion obtained still receives additional notes, in the sense that this fair opinion without exclusion is accompanied by a special explanatory note as material for improvement in the financial statements of the Regional Government in the future. This study was conducted to examine the influence of ihsan personality, work environment, and partial internal control on the quality of financial statements of the Local Government of Jepara Regency, Central Java. The population in this study is 65 institutions in Government Organizations that perform accounting and finance functions on. The samples used are 22 Sections which include 17 Government institutions and 5 agencies. The method in this study uses quantitative methods with descriptive analysis techniques and multiple linear regression analysis as well as sampling with non-probability sampling techniques with purposive sampling. The results of this study explain that ihsan's personality has a positive effect on the quality of financial statements. Furthermore, the work environment does not have a positive effect on the quality of financial statements, and the last internal control is obtained to have a positive effect on the quality of financial statements at the Jepara Local Government Organization. This research contribution provides recommendations to local governments to improve transparency, accountability, and integrity in the preparation of financial statements. In addition, this study can also provide new insights for researchers and practitioners in the field of government accounting to pay more attention to aspects of individual personality in an effort to improve the quality of financial statements.
Antifungal effect of the edible coating added with cinnamon essential oil (Cinnamomum verum) on the shelf life of strawberry (Fragaria ananassa)
Authors: Colín-Álvarez Ma. de L. , Rentería-Ortega Minerva , Barrios-Francisco R. , León-Espinosa E.B. , Castañeda-Serna H.U. 
Published: February 2025

ABSTRACT

Objective: Develop an edible coating with cinnamon essential oil (CEO) to prolong the useful life of strawberries.
Design/methodology/approach: Obtaining CEO by the distillation method with water and steam, determining the minimum inhibitory concentration through an in vitro test using the agar diffusion technique, and determining antifungal activity in vivo.
Results: The CEO was obtained by the distillation method with water and steam, showing yields of 1.2±0.3% per extraction cycle. In turn, the in vitro test on the minimum inhibitory concentration that the CEO presented against the fungus Rizhopus stolonifer using the agar dilution technique showed that its growth was completely inhibited at a concentration of 500 ppm. However, the antifungal activity in vivo is less effective due to the complex host/antifungal/pathogen system added to the temperature factor. The study at room temperature (27°C) showed growth of the fungus Rizhopus stolonifer on the strawberries with the coating at 100 and 300 ppm concentrations on day 3 of treatment. On the contrary, at refrigeration temperature (8°C), the strawberries coated with the different concentrations of CEO remained in good condition for a longer time, reflecting in their visual appearance without loss of color, the appearance of freshness, in addition to having a visually attractive shine.
Findings/conclusions: Synergy of temperature and CEO concentrations to slow the growth of Rizhopus stolonifer and prolong the life time of the fruit, maintaining its visual appearance without loss of color, the appearance of freshness and visually attractive shine.
Підготовка сержантського складу в умовах сучасної війни: виклики та перспективи
Authors: Кузьменко Р. 
Published: February 2025

Сучасна війна в Україні поставила перед Збройними Силами України безпрецедентні виклики. Швидкі темпи змін на полі бою, застосування нових видів озброєння та гібридні методи ведення війни вимагають від українських військових високого рівня професіоналізму та адаптивності. Особливо гостро стоїть питання підготовки сержантського складу, який є хребтом будь-якої армії.
Сержант – це не просто виконавець наказів, а й лідер, який здатний приймати самостійні рішення в складних бойових ситуаціях, мотивувати особовий склад та забезпечувати виконання завдань. Однак, існуюча система підготовки сержантів в Україні зіштовхнулася з низкою проблем: дефіцит досвідчених інструкторів, недостатнє фінансування, швидкі темпи змін, недостатня увага до психологічної підготовки.
Для вирішення цих проблем необхідно вжити комплекс заходів, зокрема, це швидке перенавчання та підвищення кваліфікації, тобто розробка інтенсивних програм перенавчання для сержантів з урахуванням сучасних викликів. Наступним елементом є залучення ветеранів. Активне залучення досвідчених ветеранів бойових дій для передачі своїх знань і навичок молодому поколінню. Ще одним важливим елементом є модернізація навчальної бази. Оснащення навчальних закладів сучасними тренажерами, симуляторами та іншими засобами навчання. Не менш важливим елементом є розширення міжнародного співробітництва, зокрема залучення досвіду країн-партнерів, участь у спільних навчаннях та обмін найкращими практиками.
Перспективи подальших досліджень полягають у аналізі ефективності нових методів навчання, дослідженні впливу психологічних факторів на ефективність підготовки, розвитку системи оцінювання знань та навичок, вивчення досвіду інших країн.
Загалом, можна констатувати, що система підготовки сержантів в Україні знаходиться в стадії активного розвитку. Однак, для досягнення максимальної ефективності необхідно продовжувати вдосконалювати існуючі механізми та розробляти нові підходи.
Interaction of integrin αvβ3 and fibronectin under fluid shear forces: implications for tumor cell adhesion and migration
Authors: Zhuo Paimin , Li Quhuan , Yang Bishan , Li Na , Luo Zhiqing , Zhang Fengxia 
Published: February 2025

The interaction between integrin αvβ3 and fibronectin enables tumor cell adherence to endothelial layers under diverse hydrodynamic blood flow conditions, particularly in low shear stress regions. Understanding the mechanical binding characteristics between integrin αvβ3 and fibronectin under different hydrodynamic environments can provide insights into tumor cell invasion and proliferation. Here, the adhesive behavior of fibronectin-functionalized microspheres on integrin αvβ3-coated substrates under various wall fluid shear forces (0.1–0.7 dyn/cm2) was assessed using a parallel plate flow chamber system. The bond lifetimes of integrin αvβ3-fibronectin initially increased and then decreased, indicating transition from a “catch bond” to “slip bond.” Upon perfusion of fibronectin-coated microspheres into flow chambers with high-density integrin αvβ3 coating, the rolling velocity of the microspheres increased with increasing shear force. Additionally, the mean stop time and stop frequency exhibited a force-dependent biphasic pattern, initially increasing and then decreasing with shear force, demonstrating a nuanced response to mechanical forces. Thus, the integrin αvβ3–fibronectin interaction displays a “catch bond” property, influencing cell distribution in varying fluid shear forces by promoting optimal adhesion in specific shear sites. These insights enhance our understanding of tumor cell adhesion and migration in hydrodynamic environments and may aid the design of integrin αvβ3-targeted therapies.
Identification of mouse and human embryonic pancreatic cells with adult Procr+ progenitor transcriptomic and epigenomic characteristics
Authors: Heidenreich Ana C. , Bacigalupo Lucas , Rossotti Martina , Rodríguez-Seguí Santiago A. 
Published: February 2025

BackgroundThe quest to find a progenitor cell in the adult pancreas has driven research in the field for decades. Many potential progenitor cell sources have been reported, but so far this is a matter of debate mainly due to reproducibility issues. The existence of adult Procr+ progenitor cells in mice islets has been recently reported. These were shown to comprise ~1% of islet cells, lack expression of Neurog3 and endocrine hormones, and to be capable of differentiating into all endocrine cell types. However, these findings had limited impact, as further evidence supporting the existence and function of Procr+ progenitors has not emerged.Methods and findingsWe report here an unbiased comparison across mouse and human pancreatic samples, including adult islets and embryonic tissue, to track the existence of Procr+ progenitors originally described based on their global gene expression signature. We could not find Procr+ progenitors on other mouse or human adult pancreatic islet samples. Unexpectedly, our results revealed a transcriptionally close mesothelial cell population in the mouse and human embryonic pancreas. These Procr-like mesothelial cells of the embryonic pancreas share the salient transcriptional and epigenomic features of previously reported Procr+ progenitors found in adult pancreatic islets. Notably, we report here that Procr-like transcriptional signature is gradually established in mesothelial cells during mouse pancreas development from E12.5 to E17.5, which has its largest amount. Further supporting a developmentally relevant role in the human pancreas, we additionally report that a transcriptionally similar population is spontaneously differentiated from human pluripotent stem cells cultured in vitro along the pancreatic lineage.ConclusionsOur results show that, although the previously reported Procr+ progenitor cell population could not be found in other adult pancreatic islet samples, a mesothelial cell population with a closely related transcriptional signature is present in both the mouse and human embryonic pancreas. Several lines of evidence presented in this work support a developmentally relevant function for these Procr-like mesothelial cells.
Genetic and metabolic characterization of individual differences in liver fat accumulation in Atlantic salmon
Authors: Horn Siri S. , Sonesson Anna K. , Krasnov Aleksei , Aslam Muhammad L. , Hillestad Borghild , Ruyter Bente 
Published: February 2025

IntroductionLipid accumulation in the liver can negatively impact liver function and health, which is well-described for humans and other mammals, but relatively unexplored in Atlantic salmon. This study investigates the phenotypic, genetic, and transcriptomic variations related to individual differences in liver fat content within a group of slaughter-sized Atlantic salmon reared under the same conditions and fed the same feed. The objective was to increase the knowledge on liver fat deposition in farmed salmon and evaluate the potential for genetic improvement of this trait.MethodsThe study involved measuring liver fat content in a group of slaughter-sized Atlantic salmon. Genetic analysis included estimating heritability and conducting genome-wide association studies (GWAS) to identify quantitative trait loci (QTLs). Transcriptomic analysis was performed to link liver fat content to gene expression, focusing on genes involved in lipid metabolic processes.ResultsThere was a large variation in liver fat content, ranging from 3.6% to 18.8%, with frequent occurrences of high liver fat. Livers with higher levels of fat had higher proportions of the fatty acids 16:1 n-7, 18:2 n-6, and 18:1 n-9, and less of the long-chain omega-3 fatty acids. The heritability of liver fat was estimated at 0.38, and the genetic coefficient of variation was 20%, indicating substantial potential for selective breeding to reduce liver fat deposition in Atlantic salmon. Liver fat deposition appears to be a polygenic trait, with no large QTLs detected by GWAS. Gene expression analysis linked liver fat content to numerous genes involved in lipid metabolic processes, including key transcription factors such as LXR, SREBP1, and ChREBP.DiscussionThe results indicated a connection between liver fat and increased cholesterol synthesis in Atlantic salmon, with potentially harmful free cholesterol accumulation. Further, the gene expression results linked liver fat accumulation to reduced peroxisomal β-oxidation, increased conversion of carbohydrates to lipids, altered phospholipid synthesis, and possibly increased de novo lipogenesis. It is undetermined whether these outcomes are due to high fat levels or if they are caused by underlying metabolic differences that result in higher liver fat levels in certain individuals. Nonetheless, the results provide new insights into the metabolic profile of livers in fish with inherent differences in liver fat content.
Proteomic analysis reveals candidate molecules to mediate cortical pathology and identify possible biomarkers in an animal model of multiple sclerosis
Authors: Silva Berenice Anabel , Leal María Celeste , Farias María Isabel , Nava Agustín , Galván Daniela Inés , Fernandez Elmer , Pitossi Fernando Juan , Ferrari Carina Cintia 
Published: February 2025

IntroductionMultiple Sclerosis (MS) is a complex neurodegenerative disease marked by recurring inflammatory episodes, demyelination, axonal damage, and subsequent loss of function. MS presents a wide range of clinical courses, with the progressive forms leading to irreversible neurological disability. Cortical demyelinating lesions are central to the pathology of these progressive forms, gaining critical importance in recent decades due to their strong correlation with physical disability and cognitive decline. Despite this, the underlying mechanisms driving cortical lesion formation remain poorly understood, and no specific treatments are currently available. A significant challenge lies in the lack of animal models that accurately mirror the key characteristics of these lesions.MethodsWe developed a focal cortical animal model that replicates many features of cortical lesions, including cognitive impairment. This study focuses on conducting proteomic analyses of both the cortical lesions and cerebrospinal fluid (CSF) from these animals, aiming to identify key proteins and biomarkers that could be validated in MS patients. ResultsProteomic differences between frontal cortex tissue and CSF were observed when comparing experimental animals with controls. Among the identified proteins, some have been previously described in MS patients and animal models, while others represent novel discoveries. Notably, we identified two proteins, S100A8 and orosomucoid-1, that were highly expressed in both regions. ConclusionsThese findings suggest that the prognostic molecules identified in this model could facilitate the discovery of new biomarkers or key molecules relevant to MS, particularly in the cortical lesion that mainly characterized the progressive forms of the disease.
Elevated levels of IRF1 and CASP1 as pyroptosis-related biomarkers for intestinal epithelial cells in Crohn’s disease
Authors: Xu Xiaofang , Lv Xiaodan , Zeng Ruizhi , Huang Zhixi , Huang Ziqian , Han Bing , Lin Guangfu , Lin Jianing , Li Shiquan , Fan Junhua , Lv Xiaoping 
Published: February 2025

IntroductionCrohn’s disease (CD) is a complex inflammatory condition with the potential for severe complications. Pyroptosis is an inflammatory form of programmed cell death, and the role of pyroptosis in intestinal epithelial cells of CD remains unclear.MethodsIn this study, pyroptosis-related hub genes were identified using datasets from the Gene Expression Omnibus database through differential expression analysis, machine learning algorithms, and single-cell sequencing analysis. Hub gene expression was validated using clinical samples and a trinitrobenzene sulfonic acid (TNBS)-induced colitis rat model.ResultsSix pyroptosis-related hub genes (CASP1, IRF1, ZBP1, MLKL, MMP1, HTRA1) were identified. IRF1 and CASP1 exhibited significant upregulation in CD, including both colonic and ileal subtypes, with good diagnostic value across different CD subtypes. Additionally, these two genes were not elevated in any other intestinal disorders, except for ulcerative colitis. Single-cell sequencing analysis revealed a significant interaction between intestinal epithelial cells (IECs) and monocytes. The clinical samples further confirmed that the mRNA levels of IRF1 and CASP1 were significantly higher in CD patients compared to healthy controls. Additionally, the colitis rat model validated the upregulation of Irf1 and Casp1 at both mRNA and protein levels.ConclusionOur findings identified IRF1 and CASP1 as critical pyroptosis-related biomarkers for IECs in CD, contributing to the understanding of pyroptosis in CD pathogenesis.
A prolonged activated partial thromboplastin time indicates poor short-term prognosis in patients with hepatic encephalopathy: insights from the MIMIC database
Authors: Zhan Liping , Yang Yuping , Nie Biao , Kou Yanqi , Du Shenshen , Tian Yuan , Huang Yujie , Ye Ruyin , Huang Zhe , Luo Botao , Ge Lei , Ye Shicai 
Published: February 2025

ObjectivesThis study investigates serum markers for short-term prognosis in hepatic encephalopathy patients.BackgroundPatients with hepatic encephalopathy face elevated mortality rates and bleak prognoses. However, effective prognostic models or indicators are lacking. This study aims to explore serum markers for predicting short-term prognosis in these patients.MethodsWe conducted a retrospective analysis of 552 patients with hepatic encephalopathy, categorizing 429 individuals meeting exclusion criteria into normal and high activated partial thromboplastin time (APTT) groups. We assessed 12-day and 25-day survival rates using Kaplan–Meier analysis and Cox regression models to examine associations between groups and outcomes.ResultsUpon comparing baseline characteristics, the high APTT group exhibited significant disparities in acute kidney injury, sepsis, coagulation disorders, and ascites (p &lt; 0.05). In the multivariate COX regression model, the hazard ratios [HRs; 95% confidence interval (CI)] of 12- and 25-day mortality were 1.012 (1.001, 1.022, p = 0.033) and 1.010 (1.002, 1.018, p = 0.013), respectively. We discovered that APTT demonstrated an independent association with prognosis. Our findings revealed that the ability of APTT to predict short-term prognosis surpasses that of the traditional MELD model. Regarding 12- and 25-day survival, Kaplan–Meier survival curves from these groups demonstrated a lower survival probability for patients in the high APTT group than the normal group (log-rank p &lt; 0.05). The results of subgroup analysis and interaction analysis indicate that APTT is not influenced by other confounding factors.ConclusionA prolonged APTT suggests a poorer short-term prognosis in patients with hepatic encephalopathy.
Urine complement analysis implies complement activation is involved in membranous nephropathy
Authors: Xu Yingxue , Li Yi , Zhang Yong , Li Guisen 
Published: February 2025

BackgroundThe onset and progression of membranous nephropathy (MN) have been associated with complement activation, yet the overall characteristics of this activation in the kidneys remain unclear. In our study, we utilized urine proteomic data to investigate the features of complement activation. We examined the relationship between urine complement components and both clinicopathological features and clinical outcomes in patients with MN.MethodsDifferential expression proteins (DEPs) analysis was performed using proteomic data from urine samples collected from 50 patients with MN, 50 patients with IgA nephropathies (IgAN), and 72 healthy controls (HC). Then, Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were carried out on the DEPs identified in MN. We further investigated the differentially expressed urinary complement proteins in MN patients, exploring their relationships with clinicopathological features and clinical remission. Next, 11 representative complements were selected for validation. Immunohistochemistry and immunofluorescence techniques were employed to compare the expression of CD59 and C5b-9 in renal tissues from MN patients, with analyses conducted on both the clinical remission group and the no remission group (n = 6 in each group).ResultsTotal 1,427 differentially expressed proteins were identified between the MN and HC groups. KEGG pathway analysis showed significant enrichment of these DEPs in the complement-activated pathway within the MN group. Additionally, a correlation was found between proteinuria and the levels of 27 urinary complement proteins. Notably, Collectin12 (collec12) and C1s were positively correlated with tubular atrophy/interstitial fibrosis (TIF) and monocyte infiltration. Furthermore, urine CD59 emerged as a predictor of clinical remission. Lower deposition of C5b-9 in renal tissue and higher expression of CD59 were detected in clinical remission group than non-remission group.ConclusionIn patients with MN, abnormal levels of complement components in urine are commonly observed. Currently, the use of complement inhibitors has brought new hope for the treatment of MN. The factor B inhibitor LNP023 and the factor D inhibitor BCX9930 are undergoing clinical trials for the treatment of MN. Our study indicates that complement abnormalities could serve as clinical biomarkers for tracking the progression of MN, predicting clinical remission, and guiding targeted complement therapy for those affected.
Association between cigarette smoking and the risk of major psychiatric disorders: a systematic review and meta-analysis in depression, schizophrenia, and bipolar disorder
Authors: Hu Zhonghou , Cui Enxiu , Chen Bo , Zhang Mi 
Published: February 2025

BackgroundThe prevalence of cigarette smoking among patients with major psychiatric conditions is significantly higher than that in the general population. However, whether there is a causal association between cigarette smoking and major psychiatric disorders remains unclear. Therefore, we conducted a systematic review and meta-analysis of cohort studies to elucidate the association between cigarette smoking and the risk of major psychiatric disorders, including depression, schizophrenia, and bipolar disorder.MethodsWe systematically searched PubMed, Embase, and the Cochrane Library for potentially eligible studies from their inception until March 2, 2024. All pooled analyses were performed using a random-effects model, and exploratory sensitivity and subgroup analyses were conducted.ResultsTwenty-five cohort studies involving 2,917,030 individuals were included in the meta-analysis. The summary results indicated that both current smoking (relative risk [RR], 1.30; 95% confidence interval [CI], 1.18–1.43; P &lt; 0.001) and former smoking (RR, 1.16; 95% CI, 1.09–1.23; P &lt; 0.001) were associated with an elevated risk of major depression. Additionally, current smoking was significantly associated with an elevated risk of schizophrenia (RR, 1.84; 95% CI, 1.07–3.19; P = 0.028) and bipolar disorder (RR, 1.54; 95% CI, 1.22–1.95; P &lt; 0.001).ConclusionCurrent smoking is significantly associated with an elevated risk of major psychiatric disorders, including major depression, schizophrenia, and bipolar disorder. Former smokers also have an elevated risk of major depression. However, it should be noted that, despite these significant associations, due to the nature of the cohort studies included, this study cannot establish a causal relationship between cigarette smoking and major psychiatric disorders.Systematic review registrationhttps://inplasy.com/inplasy-2024-3-0093/, identifier INPLASY202430093.