bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abstract LB-181: Piperlongumine inhibits growth and survival of human Burkitt lymphoma by downregulating NF-κB, c-Myc and LMP1
Authors: Han Seong-Su , Son Dong-Ju , Yun Hwakyung , Janz Siegfried 
Published: August 2012

Abstract
Piperlongumine (PL), a natural product constituent of the fruit of the Long pepper, Piper longum, has well documented anti-inflammatory and cancer inhibiting properties. The latter include evidence that PL selectively kills solid tumor cells, but spares their normal counterparts. Because it has not been established whether PL can also inhibit hematological malignancies of the B-lymphocyte lineage, we here decided to evaluate the efficacy with which PL suppresses growth and survival of human non-Hodgkin's Burkitt lymphoma (BL) cells in vitro. We found that PL curbed cell growth (IC50 values of PL for 24h treatment: DG-75; 8.5uM, Daudi; 2.84uM, Raji; 7.7uM and Ramos; 4.5uM) and caused Caspase-3-dependent apoptosis of BL cells in a concentration- and time-dependent manner. PL strongly suppressed constitutive NF-κB signaling by inhibition of IκBα degradation and subsequent nuclear translocation of p65 as well as interruption of NF-κB binding to its consensus DNA sequence. PL also disrupted the expression of NF-κB target genes such as E2F1, c-Myb and GADD45B. c-Myc, transcription factor important for BL development and survival, was remarkably reduced after treatment with PL. In EBV-positive BL cell lines that express high level of LMP1 (e.g., Daudi, Raji), PL efficiently repressed LMP1, a known activator of NF-κB. Considering that NF-κB, c-Myc and LMP1 play important roles in the development and in vivo maintenance of B-lineage and other types of blood cells neoplasms, our findings strongly suggest that PL may afford new approaches to the chemoprevention and treatment of blood cancers.
Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-181. doi:1538-7445.AM2012-LB-181
Abstract LB-328: Plasma antibodies to oral pathogen and commensals and risk of pancreatic cancer in a large European prospective cohort study
Authors: Michaud Dominique S. , Izard Jacques , Wilhelm-Benartzi Charlotte , Riboli Elio , Bueno-de-Mesquita Bas ,   for the EPIC team.
Published: August 2012

Abstract
Background: A positive relationship between periodontal disease and pancreatic cancer risk has been observed in previous epidemiological studies. In a cohort study of health professional men, never smokers reporting a positive history of periodontal disease had a 2-fold higher risk of pancreatic cancer compared with those reporting no periodontal disease (RR = 2.09, 95% CI = 1.18-3.71). These findings suggest that oral bacteria may be able to disseminate to the pancreas or may be associated with risk indirectly. We examined the relationship between the presence of antibodies to 25 diverse strains of oral bacteria and pancreatic cancer risk in a prospective study. Methods: Antibodies to oral bacteria were measured in prediagnostic blood samples of 405 pancreatic cancer cases and 416 matched controls nested within the European Prospective Investigation on Cancer (EPIC), a large cohort study. Analyses were conducted using conditional logistic regression and additionally adjusted for smoking status and body mass index. Results: Individuals with high antibody levels to Porphyromonas gingivalis ATTC 53978 (&gt;200 ng/ml), a pathogenic periodontal bacteria, had a two-fold higher risk of pancreatic cancer (OR = 2.14, 95% CI =1.05-4.36) compared with individuals with low levels of antibody (&lt;200ng/ml). To explore the association with common oral bacteria, we performed a cluster analysis and obtained two clusters that aggregated individuals according to their antibody profiles. A substantially lower risk of pancreatic cancer was observed in the cluster with consistently elevated levels of antibodies, compared to a second cluster with overall lower levels (OR = 0.55, 95% CI = 0.36-0.83). Conclusions: In this study using prediagnostic bloods, we find support for a positive association between periodontal disease and pancreatic cancer. Additionally, our results further suggest that certain oral bacteria or an enhanced immune surveillance may provide some protection against pancreatic cancer. Our findings are consistent with dental research on periodontal disease etiology and treatment. Whether periodontal disease is causally related to pancreatic cancer or simply a marker of underlying immune response needs to be further elucidated.
Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-328. doi:1538-7445.AM2012-LB-328
Abstract LB-87: Methylated-mediated repression of ZNF154 in ovarian cancer is associated with poor overall survival
Authors: Okamoto Takako , Yamaguchi Ken , Huang Zhiqing , Whitaker Regina , Konishi Ikuo , Berchuck Andrew , Murphy Susan K. 
Published: August 2012

Abstract
Objectives: Epigenetic gene regulation plays an important role in the development of cancer. We previously found that Zinc Finger Protein 154 (ZNF154), a Krüppel family zinc finger protein, is ubiquitously methylated in a large panel of ovarian cancer cell lines but is unmethylated in normal cells. The objectives of this study were to determine the relevance of ZNF154 methylation to mRNA expression and relationship to clinical outcome in primary epithelial ovarian cancers. Methods: Genomic DNA from 85 ovarian cancers (53 serous, 13 clear cell, 11 endometrioid and 8 mucinous) along with 4 fallopian tube and 4 ovarian surface epithelium specimens was bisulfite modified (Zymo EZ DNA Methylation kit) followed by generation of Illumina Infinium 450k Methylation Beadchip data for &gt;485,000 CpG sites, given as β-values (scale, 0-1, where 1 is completely methylated). Bisulfite pyrosequencing was used to validate array results. ZNF154 mRNA expression was quantified using real time RT-PCR following treatment of 5 ovarian cancer cell lines and 2 immortalized OSE controls with 5µM 5-aza-2′ deoxycytidine (DAC) for 72h. Mann-Whitney U tests were used for group comparisons, Pearson correlation for continuous variables and the Kaplan-Meier method for overall survival. Results: Methylation of ZNF154 was significantly higher in cancers versus normal tissues (average β=0.51 versus β=0.09, respectively; p&lt;0.0001). Methylation values obtained by pyrosequencing strongly correlated with array β-values (R=0.94; p=1.31e-40). ZNF154 mRNA expression was lower in ovarian cancer versus normal cells (p=0.015). Following DAC treatment, ZNF154 expression increased an average 8.01-fold (p=0.0499) while methylation levels decreased up to 20% (p=0.028) compared to mock treated controls. Stage III-IV serous cancers with higher (N=25) versus lower (N=22) ZNF154 methylation had 66 months decreased median overall survival (p=0.032). Conclusions: ZNF154 is ubiquitously methylated in primary ovarian cancers but not in normal cells and expression of ZNF154 is at least partially regulated by DNA methylation. In addition, increased methylation of ZNF154 is associated with poor clinical outcome for advanced stage serous cancers. Our results suggest that ZNF154 methylation status may have utility as a biomarker and also as a tool for prognostic prediction.
Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr LB-87. doi:1538-7445.AM2012-LB-87
Myocardial Systolic and Diastolic Performance Derived by 2-Dimensional Speckle Tracking Echocardiography in Heart Failure With Normal Left Ventricular Ejection Fraction
Authors: Morris Daniel A. , Boldt Leif-Hendrik , Eichstädt Hermann , Özcelik Cemil , Haverkamp Wilhelm 
Published: August 2012

Background—
The aim of this study was to investigate the myocardial systolic and diastolic performance of the left ventricle (LV) in patients with heart failure with normal LV ejection fraction (HFNEF) through novel LV myocardial indices, which assess the systolic and diastolic function of the whole myocardium of the LV.

Methods and Results—

LV myocardial systolic and diastolic performance were assessed as the average value of peak systolic strain and peak early-diastolic strain rate, respectively, in longitudinal, circumferential, and radial directions from all LV segments using 2-dimensional speckle-tracking echocardiography. We studied patients with HFNEF and a control group consisting of asymptomatic subjects with LV diastolic dysfunction of similar age, sex, and LV ejection fraction. A total of 322 patients were included (92 with HFNEF and 230 with asymptomatic LV diastolic dysfunction). Myocardial systolic and diastolic LV performance were significantly lower in HFNEF (20.13±6.02% and 1.14±0.27 s
−1
) than in patients with asymptomatic LV diastolic dysfunction (25.33±6.06% and 1.37±0.33 s
−1
, respectively; all
P
<0.0001). In addition, patients with HFNEF with low systolic and diastolic LV myocardial performance had significantly higher LV filling pressures (17.1±6.6 and 17.6±6.3 versus 12.0±5.1 and 11.7±4.7, respectively; all
P
<0.001) and lower cardiac output (4.8±1.0 L/min and 4.9±1.1 L/min versus 5.7±1.2 L/min and 5.8±1.1 L/min, respectively; all
P
<0.001) than patients with normal LV myocardial performance. In relation to these findings, the symptomatic status (ie, New York Heart Association functional class) was significantly altered in those patients with low systolic and diastolic LV myocardial performance.

Conclusions—
In patients with HFNEF, both systolic and diastolic LV myocardial performance are impaired, which is associated with increased LV filling pressures, decreased cardiac output, and worse New York Heart Association functional class. Therefore, the measurement of these myocardial parameters could be of great importance in HFNEF because these echocardiographic indices assess the multidirectional function of the whole myocardium of the LV, thereby allowing detection of an alteration of the global function of the LV which is associated with a worse symptomatic status in these patients.
Missed Opportunities
Authors: Subherwal Sumeet , Patel Manesh R. , Kober Lars , Peterson Eric D. , Jones William S. , Gislason Gunnar H. , Berger Jeffrey , Torp-Pedersen Christian , Fosbol Emil L. 
Published: August 2012

Background—
Patients with peripheral artery disease (PAD) are at high risk of cardiovascular events and benefit from aggressive secondary prevention; however, changes in the use of cardioprotective medications after incident diagnosis of PAD have not been well described.

Methods and Results—

We used Danish nationwide administrative registries (2000–2007) to identify 2 groups with incident PAD: PAD alone (n=34 160) and PAD with history of coronary artery disease (CAD) (n=9570). With the use of a comparator with incident CAD alone (n=154 183), we assessed temporal trends and comparative use of cardioprotective medications. Relative differences in medication use were examined by using multivariable logistic regression. Use of medications improved temporally among both groups: for PAD alone, any antiplatelet use increased from 29% to 59% from 2000 to 2007 (
P
<0.0001), whereas statin use increased 6-fold (9%–56%;
P
<0.0001). However, use of these therapies by 18 months after incident diagnosis for both PAD groups remained modest and lower in comparison with CAD alone (any antiplatelet, 53% versus 66%; statins, 40% versus 52%; angiotensin-converting enzyme inhibitors, 20% versus 29%). Relative to CAD alone, patients with PAD alone were less likely to use any antiplatelet (adjusted odds ratio, 0.50; 95% confidence interval, 0.49–0.52), statins (adjusted odds ratio, 0.50; 95% confidence interval, 0.48–0.52), or angiotensin-converting enzyme inhibitors (adjusted odds ratio, 0.51; 95% confidence interval, 0.49–0.53) by 18 months.

Conclusions—
Despite improvement in the use of cardioprotective medications over time, patients with PAD alone remain less likely than those with CAD alone to use these agents.
Seasonal Prediction of Killing-Frost Frequency in South-Central Canada during the Cool/Overwintering-Crop Growing Season
Authors: Wu Zhiwei , Lin Hai , Li Yun , Tang Youmin 
Published: August 2012

AbstractSeasonal killing-frost frequency (KFF) during the cool/overwintering-crop growing season is important for the Canadian agricultural sector to prepare and respond to such extreme agrometeorological events. On the basis of observed daily surface air temperature across Canada for 1957–2007, this study found that more than 86% of the total killing-frost events occur in April–May and exhibit consistent variability over south-central Canada, the country’s major agricultural region. To quantify the KFF year-to-year variations, a simple index is defined as the mean KFF of the 187 temperature stations in south-central Canada. The KFF variability is basically dominated by two components: the decadal component with a peak periodicity around 11 yr and the interannual component of 2.5–3.8 yr. A statistical method called partial least squares (PLS) regression is utilized to uncover principal sea surface temperature (SST) modes in the winter preceding the KFF anomalies. It is found that most of the leading SST modes resemble patterns of El Niño–Southern Oscillation (ENSO) and/or the Pacific decadal oscillation (PDO). This indicates that ENSO and the PDO might be two dominant factors for the KFF variability. From a 41-yr training period (1957–97), a PLS seasonal prediction model is established, and 1-month-lead real-time forecasts are performed for the validation period of 1998–2007. A promising skill level is obtained. For the KFF variability, the prediction skill of the PLS model is comparable to or even better than the newly developed Canadian Seasonal to Interannual Prediction System (CanSIPS), which is a state-of-the-art global coupled dynamical system.
Measuring the medical school educational environment: Validating an approach from Saudi Arabia
Authors: Alshehri Sarah A , Alshehri Abdulrahman F , Erwin T Dary 
Published: August 2012

Objective: This study is an empirical analysis of the female students’ attitudes toward the medical educational environment and climate in the College of Medicine at King Khalid University. Setting: The Dundee Ready Educational Environment Measure (DREEM) questionnaire was administered on the same day to 100 female students studying in the third (pre-clinical), fifth and sixth (final) year at the College of Medicine, King Khalid University. Although the sample size was limited in size, it was deemed acceptable for research purposes. Method: The study is conducted using the DREEM questionnaire. This study is different from other studies regarding testing hypothesis and advanced statistical analysis. A set of hypotheses were placed and examined using statistical techniques. Results: In general, we find that the total mean score was 112.95 out of a 200 maximum, the ideal educational environment as measured by this scale. All the hypotheses set were rejected and there was no difference in the results according to year of study such as the difference found in some previous studies. It was found that there are no statistically significant differences in the mean scores according to the year of study. Conclusion: This study follows a long literature of studies in different universities around the world using the DREEM scale. The limitations of previous studies were considered carefully and were used to provide a framework for this work. These results spotlight the need to improve the medical educational environment in Saudi universities in general. This study has highlighted some areas where further research and analysis is recommended.
Migraine, weight gain and the risk of becoming overweight and obese: A prospective cohort study
Authors: Winter Anke C , Wang Lu , Buring Julie E , Sesso Howard D , Kurth Tobias 
Published: August 2012

Background: Some cross-sectional studies have suggested an association between migraine and increased body weight. However, prospective data on the association are lacking. Methods: We conducted a prospective cohort study among 19,162 participants in the Women’s Health Study who had a body mass index (BMI) of 18.5– <25 kg/m2 at baseline. Migraine was self-reported by standardized questionnaires. Main outcome measures were incident overweight (BMI ≥ 25 kg/m2), incident obesity (BMI ≥ 30 kg/m2) and mean weight change. Age- and multivariable-adjusted hazard ratios (HRs) were calculated for the association between migraine and incident overweight and obesity. Differences in weight change were evaluated by analysis of covariance (ANCOVA). Results: A total of 3,483 (18.2%) women reported any migraine history. After 12.9 years of follow-up, 7916 incident overweight and 730 incident obesity cases occurred. Migraineurs had multivariable-adjusted HRs (95% confidence interval) of 1.11 (1.05–1.17) for becoming overweight and 1.00 (0.83–1.19) for becoming obese. These associations remained stable after censoring for chronic diseases and were similar according to migraine aura status. Multivariable-adjusted mean weight change from baseline to the end of study was +4.7 kg for migraineurs and +4.4 kg for women without migraine ( p = 0.02). Conclusion: Results of this large prospective study of middle-aged women do not indicate a consistent association between migraine and incident overweight, obesity or relevant weight gain.
Minimal residual disease monitoring by quantitative RT-PCR in core binding factor AML allows risk stratification and predicts relapse: results of the United Kingdom MRC AML-15 trial
Authors: Yin John A. Liu , O'Brien Michelle A. , Hills Robert K. , Daly Sarah B. , Wheatley Keith , Burnett Alan K. 
Published: August 2012

AbstractThe clinical value of serial minimal residual disease (MRD) monitoring in core binding factor (CBF) acute myeloid leukemia (AML) by quantitative RT-PCR was prospectively assessed in 278 patients [163 with t(8;21) and 115 with inv(16)] entered in the United Kingdom MRC AML 15 trial. CBF transcripts were normalized to 105ABL copies. At remission, after course 1 induction chemotherapy, a > 3 log reduction in RUNX1-RUNX1T1 transcripts in BM in t(8;21) patients and a > 10 CBFB-MYH11 copy number in peripheral blood (PB) in inv(16) patients were the most useful prognostic variables for relapse risk on multivariate analysis. MRD levels after consolidation (course 3) were also informative. During follow-up, cut-off MRD thresholds in BM and PB associated with a 100% relapse rate were identified: for t(8;21) patients BM > 500 copies, PB > 100 copies; for inv(16) patients, BM > 50 copies and PB > 10 copies. Rising MRD levels on serial monitoring accurately predicted hematologic relapse. During follow-up, PB sampling was equally informative as BM for MRD detection. We conclude that MRD monitoring by quantitative RT-PCR at specific time points in CBF AML allows identification of patients at high risk of relapse and could now be incorporated in clinical trials to evaluate the role of risk directed/preemptive therapy.
Different metastatic pattern according to the KRAS mutational status and site-specific discordance of KRAS status in patients with colorectal cancer
Authors: Kim Mi-Jung , Lee Hye Seung , Kim Jee Hyun , Kim Yu Jung , Kwon Ji Hyun , Lee Jeong-Ok , Bang Soo-Mee , Park Kyoung Un , Kim Duck-Woo , Kang Sung-Bum , Kim Jae-Sung , Lee Jong Seok , Lee Keun-Wook 
Published: August 2012

Abstract

Background
We evaluated the association between a KRAS mutational status and various clinicopathologic features including the metastatic pattern in patients with metastatic or recurrent colorectal cancer (MRCRC). The concordance rates of the KRAS status between primary tumor sites and paired metastatic organs were also analyzed.

Methods
The KRAS mutational status in codons 12, 13, and 61 from formalin-fixed sections of both primary tumors and related metastases was determined by sequencing analysis. One hundred forty-three Korean patients with MRCRC with available tissues (resection or biopsy) from both primary tumors and related metastatic sites were consecutively enrolled.

Results
The KRAS mutation rate was 52.4% (75/143) when considering both the primary and metastatic sites. When the relationship between the KRAS status and initial metastatic sites at the time of diagnosis of MRCRC was analyzed, lung metastasis was more frequent as the initial metastatic site in patients with the KRAS mutation than in patients without the KRAS mutation (45.3% vs. 22.1%; P = 0.003). However, liver (37.3% vs. 70.6%; P < 0.001) or distant lymph node metastases (6.7% vs. 19.1%; P = 0.025) were less frequent as the initial metastatic organ in patients with the KRAS mutation than in patients without the KRAS mutation. The discordance rate of KRAS mutational status between primary and paired metastatic sites other than the lung was 12.3% (13/106). Compared with primary tumor sites, the KRAS discordance rate was significantly higher in matched lung metastases [32.4% (12/37)] than in other matched metastatic organs (P = 0.005).

Conclusions
Organs initially involved by distant metastasis were different according to the KRAS mutational status in MRCRC patients. The concordance rate (87.7%) of the KRAS mutation status at metastatic sites other than the lung was generally high compared with primary tumor sites; however, lung metastasis had a high rate of KRAS discordance (32.4%).
Quality of health news disseminated in the print media in developing countries: a case study in Iran
Authors: Ashoorkhani Mahnaz , Gholami Jaleh , Maleki Katayoun , Nedjat Sima , Mortazavi Jalaledin , Majdzadeh Reza 
Published: August 2012

Abstract

Background
Mass media play an important role in keeping people up-to-date with the latest health news. This study aims at investigating the quality of health news disseminated in the print media, its course of production and factors affecting its quality.

Methods
In the quantitative section of the study, 410 health-related news items, published during a six-month span in the Iranian public press, underwent content analysis. In the qualitative section, focus group discussions were held with journalists, editors-in-chief and news gatekeepers.

Results
The quantitative phase showed that 18% of the news articles were not fit for dissemination in public. The qualitative phase illustrated that multiple factors at various levels affect the quality of news, namely poor knowledge, inadequate motivations and context-related barriers.

Conclusions
The quality of health news reporting is not desirable. Educational interventions need to be carried out to raise awareness among researchers and journalists. Also, certain steps should be taken to increase motivations and strengthen infrastructures, including designing guidelines and monitoring news.