bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rational Approximation and Analog Realization of Fractional Order Transfer Function with Multiple Fractional Powered Terms
Authors: Khanra M. , Pal J. , Biswas K. 
Published: July 2012

AbstractThe concept of model order reduction of high integer order systems using approximate moment matching has been generalized and extended to rational approximation of fractional order transfer function (FOTF). This approximation method converts an FOTF into characteristic numeric values and thus can be applied to rational approximation of FOTF, irrespective of its form of representation. This is very effective when FOTF has multiple fractional powered terms. Two circuit configurations for analog realization of FOTF through rational approximation are discussed and have been shown to be useful in hardware implementation. As a case study, a half order differentiator has been implemented in hardware and can be extended to any FOTF.
An amperometric enzyme biosensor for real‐time measurements of cellobiohydrolase activity on insoluble cellulose
Authors: Cruys‐Bagger Nicolaj , Ren Guilin , Tatsumi Hirosuke , Baumann Martin J. , Spodsberg Nikolaj , Andersen Heidi Delcomyn , Gorton Lo , Borch Kim , Westh Peter 
Published: July 2012

AbstractAn amperometric enzyme biosensor for continuous detection of cellobiose has been implemented as an enzyme assay for cellulases. We show that the initial kinetics for cellobiohydrolase I, Cel7A from Trichoderma reesei, acting on different types of cellulose substrates, semi‐crystalline and amorphous, can be monitored directly and in real‐time by an enzyme‐modified electrode based on cellobiose dehydrogenase (CDH) from Phanerochaete chrysosporium (Pc). PcCDH was cross‐linked and immobilized on the surface of a carbon paste electrode which contained a mediator, benzoquinone. An oxidation current of the reduced mediator, hydroquinone, produced by the CDH‐catalyzed reaction with cellobiose, was recorded under constant‐potential amperometry at +0.5 V (vs. Ag/AgCl). The CDH‐biosensors showed high sensitivity (87.7 µA mM−1 cm−2), low detection limit (25 nM), and fast response time (t95% ∼ 3 s) and this provided experimental access to the transient kinetics of cellobiohydrolases acting on insoluble cellulose. The response from the CDH‐biosensor during enzymatic hydrolysis was corrected for the specificity of PcCDH for the β‐anomer of cello‐oligosaccharides and the approach were validated against HPLC. It is suggested that quantitative, real‐time data on pure insoluble cellulose substrates will be useful in attempts to probe the molecular mechanism underlying enzymatic hydrolysis of cellulose. Biotechnol. Bioeng. 2012; 109: 3199–3204. © 2012 Wiley Periodicals, Inc.
Automated dynamic fed‐batch process and media optimization for high productivity cell culture process development
Authors: Lu Franklin , Toh Poh Choo , Burnett Iain , Li Feng , Hudson Terry , Amanullah Ashraf , Li Jincai 
Published: July 2012

AbstractCurrent industry practices for large‐scale mammalian cell cultures typically employ a standard platform fed‐batch process with fixed volume bolus feeding. Although widely used, these processes are unable to respond to actual nutrient consumption demands from the culture, which can result in accumulation of by‐products and depletion of certain nutrients. This work demonstrates the application of a fully automated cell culture control, monitoring, and data processing system to achieve significant productivity improvement via dynamic feeding and media optimization. Two distinct feeding algorithms were used to dynamically alter feed rates. The first method is based upon on‐line capacitance measurements where cultures were fed based on growth and nutrient consumption rates estimated from integrated capacitance. The second method is based upon automated glucose measurements obtained from the Nova Bioprofile FLEX® autosampler where cultures were fed to maintain a target glucose level which in turn maintained other nutrients based on a stoichiometric ratio. All of the calculations were done automatically through in‐house integration with a Delta V process control system. Through both media and feed strategy optimization, a titer increase from the original platform titer of 5 to 6.3 g/L was achieved for cell line A, and a substantial titer increase of 4 to over 9 g/L was achieved for cell line B with comparable product quality. Glucose was found to be the best feed indicator, but not all cell lines benefited from dynamic feeding and optimized feed media was critical to process improvement. Our work demonstrated that dynamic feeding has the ability to automatically adjust feed rates according to culture behavior, and that the advantage can be best realized during early and rapid process development stages where different cell lines or large changes in culture conditions might lead to dramatically different nutrient demands. Biotechnol. Bioeng. 2013; 110: 191–205. © 2012 Wiley Periodicals, Inc.
Outdoor cultivation of temperature‐tolerant Chlorella sorokiniana in a column photobioreactor under low power‐input
Authors: Béchet Quentin , Muñoz Raul , Shilton Andy , Guieysse Benoit 
Published: July 2012

AbstractTemperature‐tolerant Chlorella sorokiniana was cultivated in a 51‐L column photobioreactor with a 1.1 m2 illuminated area. The reactor was operated outdoors under tropical meteorological conditions (Singapore) without controlling temperature and the culture was mixed at a power input of 7.5 W/m3 by sparging CO2‐enriched air at 1.2 L/min (gas hold‐up of 0.02). Biomass productivity averaged 10 ± 2.2 g/${\rm m}_{{\rm illuminated}\,{\rm area}}^{{\rm 2}} {\rm \hbox{-} day}$ over six batch studies, yielding an average photosynthetic efficiency (PE) of 4.8 ± 0.5% of the total solar radiation (P = 0.05, N = 6). This demonstrates that temperature‐tolerant microalgae can be cultivated at high PE under a mixing input sevenfold to ninefold lower than current operational guidelines (50–70 W/m3) and without the need for temperature control (the culture broth temperature reached 41°C during operation). In this study, the PE value was determined based on the amount of solar radiation actually reaching the algae and this amount was estimated using a mathematical model fed with onsite solar irradiance data. This determination was found to be particularly sensitive to the value of the atmospheric diffusion coefficient, which generated a significant uncertainty in the PE calculation. The use of the mathematical model, however, confirmed that the vertical reactor geometry supported efficient photosynthesis by reducing the duration and intensity of photoinhibition events. The model also revealed that all three components of direct, diffuse, and reflected solar radiation were quantitatively important for the vertical column photobioreactor, accounting for 14%, 65%, and 21% of the total solar radiation reaching the culture, respectively. The accurate prediction of the discrete components of solar radiation reaching the algae as a function of climatic, geographic, and design parameters is therefore crucial to optimize the individual reactor geometry and the layout/spacing between the individual reactors in a reactor farm. Biotechnol. Bioeng. 2013; 110: 118–126. © 2012 Wiley Periodicals, Inc.
Simultaneous quantification of metformin and glipizide in human plasma by high‐performance liquid chromatography–tandem mass spectrometry and its application to a pharmacokinetic study
Authors: Li Ning , Deng Ying , Qin Feng , Yu Jia , Li Famei 
Published: July 2012

ABSTRACTA selective, sensitive and rapid high‐performance liquid chromatography–tandem mass spectrometry (HPLC‐MS/MS) method was developed and validated to determine metformin and glipizide simultaneously in human plasma using phenacetin as internal standard (IS). After one‐step protein precipitation of 200 μL plasma with methanol, metformin, glipizide and IS were separated on a Kromasil Phenyl column (4.6 × 150 mm, 5 µm) at 40°C with an isocratic mobile phase consisting of methanol–10 mmol/L ammonium acetate (75:25, v/v) at a flow rate of 0.35 mL/min. Electrospray ionization source was applied and operated in the positive mode. Multiple reaction monitoring using the precursor → product ion combinations of m/z 130 → m/z 71, m/z 446 → m/z 321 and m/z 180 → m/z 110 were used to quantify metformin, glipizide and IS, respectively. The linear calibration curves were obtained over the concentration ranges 4.10–656 ng/mL for metformin and 2.55–408 ng/mL for glipizide. The relative standard deviation of intra‐day and inter‐day precision was below 10% and the relative error of accuracy was between −7.0 and 4.6%. The presented HPLC‐MS/MS method was proved to be suitable for the pharmacokinetic study of metformin hydrochloride and glipizide tablets in healthy volunteers after oral administration. Copyright © 2012 John Wiley & Sons, Ltd.
A Modified Approach for Generating Column Grand Composite Curves
Authors: Wei Z. , Wu S. , Zhang B. , Chen Q. , Hui C.‐W. 
Published: July 2012

AbstractIn generating a column grand composite curve (CGCC) the enthalpy values calculated by top‐down and bottom‐up approaches are different at the feed stage. An insight analysis of material and energy balances indicates that these differences are due to the feed stage treated as a stripping stage in the top‐down approach and as a rectifying stage in the bottom‐up approach. To consider the practical feed process and overcome the shortcomings in the existing CGCC generation approaches, a modified approach is proposed, dividing the column into three sections: the rectifying section (from the 1st to the f‐2th stage, the condenser being the 1st stage), the feeding section (the f‐1th and the fth stage) and the stripping section (from the f+1th to the nth stage, the reboiler being the nth stage). The modification is mainly focused on the feeding section stages. An indicator, the reflux energy‐saving potential rate, is defined and used to evaluate these approaches. A benzene‐toluene column is investigated to demonstrate the performance of the proposed approach, and a comparison is made with Aspen Plus Column Targeting. The results show that the modified approach is able to eliminate the ambiguity at the feed stage. Compared to Aspen Plus Column Targeting and the existing approaches, the modified approach could give better results.
A Nucleobase‐Discriminating Pyrrolo‐dC Click Adduct Designed for DNA Fluorescence Mismatch Sensing
Authors: Ming Xin , Seela Frank 
Published: July 2012

AbstractNew pyrrolo‐dC click adducts (4 and 5) tethered with a 1,2,3‐triazole skeleton were synthesized and oligonucleotides were prepared. The triazole system was either directly linked to the pyrrolo moiety (5) or connected via an n‐butyl linker (4). The quantum yield of nucleoside 5 (Φ=0.32), which is 10 times higher than those of 8‐methylpyrrolo‐dC (1 b, Φ=0.026) or the long linker derivative 4 (Φ=0.03), is maintained in oligonucleotides. Compound 5 was used as a nucleobase‐discriminating fluorescence sensor in duplex DNA. Excellent mismatch discrimination was observed when 5 was positioned opposite the four canonical nucleosides. Compound 5 has the potential to be used for SNP detection in long DNA targets when conventional techniques such as high resolution melt analysis fail.
High Relaxivity Supramolecular Adducts Between Human‐Liver Fatty‐Acid‐Binding Protein and Amphiphilic GdIII Complexes: Structural Basis for the Design of Intracellular Targeting MRI Probes
Authors: D'Onofrio Mariapina , Gianolio Eliana , Ceccon Alberto , Arena Francesca , Zanzoni Serena , Fushman David , Aime Silvio , Molinari Henriette , Assfalg Michael 
Published: July 2012

AbstractGadolinium complexes linked to an apolar fragment are known to be efficiently internalized into various cell types, including hepatocytes. Two lipid‐functionalized gadolinium chelates have been investigated for the targeting of the human liver fatty acid binding protein (hL‐FABP) as a means of increasing the sensitivity and specificity of intracellular‐directed MRI probes. hL‐FABP, the most abundant cytosolic lipid binding protein in hepatocytes, displays the ability to interact with multiple ligands involved in lipid signaling and is believed to be an obligate carrier to escort lipidic drugs across the cell. The interaction modes of a fatty acid and a bile acid based gadolinium complex with hL‐FABP have been characterized by relaxometric and NMR experiments in solution with close‐to‐physiological protein concentrations. We have introduced the analysis of paramagnetic‐induced protein NMR signal intensity changes as a quantitative tool for the determination of binding stoichiometry and of precise metal‐ion‐center positioning in protein–ligand supramolecular adducts. A few additional NMR‐derived restraints were then sufficient to locate the ligand molecules in the protein binding sites by using a rapid data‐driven docking method. Relaxometric and 13C NMR competition experiments with oleate and the gadolinium complexes revealed the formation of heterotypic adducts, which indicates that the amphiphilic compounds may co‐exist in the protein cavity with physiological ligands. The differences in adduct formation between fatty acid and bile acid based complexes provide the basis for an improved molecular design of intracellular targeted probes.
Strong Aphicidal Activity of GlcNAc(β1→4)Glc Disaccharides: Synthesis, Physiological Effects, and Chitinase Inhibition
Authors: Dussouy Christophe , Bultel Laurent , Saguez Julien , Cherqui Anas , Khelifa Mounia , Grand Eric , Giordanengo Philippe , Kovensky José 
Published: July 2012

AbstractThe synthesis of four GlcNAc(β1→4)Glc disaccharides containing 2‐O‐acetyl and/or 6‐sulfate groups was performed in high yields with total 1,2‐trans stereoselectivity. These disaccharides were evaluated as candidates for insect chitinase inhibition and aphicidal activity. All the compounds prepared displayed physiological effects on M. persicae aphids; however, the inhibition of chitinases of different sources (bacteria, fungus, and aphid) followed different patterns according to subtle structural characteristics.
ChemInform Abstract: Hierarchical Porous NiCo2O4 Nanowires for High‐Rate Supercapacitors.
Authors: Jiang Hao , Ma Jan , Li Chunzhong 
Published: July 2012

AbstractThe title nanowires are synthesized from mixtures of Ni(NO3)2, Co(NO3)2, H2C2O4, P123, EtOH, and polyethylene glycol at room temperature (10 min) followed by annealing the obtained precipitates under air at 250 °C for 3 h.
ChemInform Abstract: Rapid Microwave Hydrothermal Synthesis of ZnGa2O4 with High Photocatalytic Activity Toward Aromatic Compounds in Air and Dyes in Liquid Water.
Authors: Sun Meng , Li Danzhen , Zhang Wenjuan , Chen Zhixin , Huang Hanjie , Li Wenjuan , He Yunhui , Fu Xianzhi 
Published: July 2012

AbstractThe title compound is synthesized by a microwave hydrothermal method from an aqueous solution of Ga(NO3)3, ZnCl2, and ammonia (microwave system, 200 W, 2.45 GHz, autoclave, 160 °C, 20 min).
ChemInform Abstract: Dumbbells of Five‐Connected Ge Atoms and Superconductivity in CaGe3.
Authors: Schnelle Walter , Ormeci Alim , Wosylus Aron , Meier Katrin , Grin Yuri , Schwarz Ulrich 
Published: July 2012

AbstractCaGe3 is synthesized by annealing mixtures of CaGe2 (obtained by reacting the elements in an arc furnace) and Ge at 10 GPa and 1200 K for 1 h.
ChemInform Abstract: A Strategy for the Synthesis of Mesostructured Metal Oxides with Lower Oxidation States.
Authors: Tueysuez Harun , Weidenthaler Claudia , Schueth Ferdi 
Published: July 2012

AbstractOrdered mesoporous CoO and Fe3O4 are synthesized by reduction of Co3O4 and ferrihydrite (Fe2O3·nH2O), respectively, using glycerol/water vapor as a reducing agent (320 °C, 2 h).
ChemInform Abstract: Intramolecular Thermal Transformations of N‐Phthalimidoaziridines: 1,3‐Dipolar Cycloaddition and Rearrangements.
Authors: Kuznetsov M. A. , Pan'kova A. S. , Voronin V. V. , Vlasenko N. A. 
Published: July 2012

AbstractThe title reaction of o‐substituted 2‐phenyl‐N‐phthalimidoaziridines affords a variety of condensed heterocyclic systems such as fused pyrrole derivatives (II), pyrrolobenzoxepine (IV) and imidazobenzopyran (VIII).
ChemInform Abstract: Expanding the Regioselective Enzymatic Repertoire: Oxidative Monocleavage of Dialkenes Catalyzed by Trametes hirsuta.
Authors: Paul Caroline E. , Rajagopalan Aashrita , Lavandera Ivan , Gotor‐Fernandez Vicente , Kroutil Wolfgang , Gotor Vicente 
Published: July 2012

AbstractThe title reaction selectively cleaves the double bond adjacent to the aromatic ring, allowing for the differentiation between two terminal alkenes and the preferential cleavage of an internal double bond over a terminal one.
ChemInform Abstract: Platinum on Carbon‐Catalyzed Precise Reduction Control of Trichloromethyl to Geminal‐Dichloromethyl Groups.
Authors: Imanishi Takahiro , Fujiwara Yuta , Sawama Yoshinari , Monguchi Yasunari , Sajiki Hironao 
Published: July 2012

AbstractA variety of aromatic and aliphatic α,α,α‐trichloromethylcarbonyl compounds are successfully monodechlorinated.
ChemInform Abstract: Enantioselective Sulfa‐Michael Addition of Thioacids to α,β‐Unsaturated Ketones with Bifunctional Organocatalyst.
Authors: Rana Nirmal K. , Unhale Rajshekhar , Singh Vinod K. 
Published: July 2012

AbstractA screening of various quinine‐/quinidine‐derived catalysts reveals that the best results are achieved in the presence of the bifunctional thiourea URC.
ChemInform Abstract: Pyrolysis of Dimethoxy[n.2]metacyclophanes: An Intramolecular Condensation Reaction to Give Oxa[n.2.1](1,3,2)cyclophanes.
Authors: Do Jung‐Hee , Yamada Yuta , Sharma Bigyan , Yamoto Takehiko 
Published: July 2012

AbstractThe pyrolysis of anti‐cyclophanes (I) and (IV) is studied and the mechanism of the novel intramolecular condensation leading to oxacyclophanes (II) and (V)/(VI), resp., is discussed.
ChemInform Abstract: Oxazolidinone‐Promoted, Torquoselective Nazarov Cyclizations.
Authors: Kerr Daniel J. , Miletic Michael , Chaplin Jason H. , White Jonathan M. , Flynn Bernard L. 
Published: July 2012

AbstractIn the presence of an excess of MesOH or TfOH, divinyl and aryl vinyl ketones bearing an oxazolidinone auxiliary undergo efficient and highly stereoselective cyclization to optically active cyclopentenone derivatives.
ChemInform Abstract: Cyclopropanation of Strained Alkenes by Palladium‐Catalyzed Reaction of 3‐Trimethylsilyl‐ or 3‐Pinacolatoboryl‐1‐arylallyl Acetates.
Authors: Horino Yoshikazu , Homura Naoki , Inoue Kana , Yoshikawa Saori 
Published: July 2012

AbstractThe title reaction of norbornadienes or norbornenes affords the cyclopropanation products as single diastereomers.
ChemInform Abstract: Ruthenium(0)‐Catalyzed sp3 C—H Bond Arylation of Benzylic Amines Using Arylboronates.
Authors: Dastbaravardeh Navid , Schnuerch Michael , Mihovilovic Marko D. 
Published: July 2012

AbstractAn efficient arylation adjacent to the free NH group in substrates (I) and (VII) includes pyridine‐directed cyclometalation with Ru3(CO)12.
ChemInform Abstract: Regioselective Cu(I)‐Catalyzed Tandem A3‐Coupling/Decarboxylative Coupling to 3‐Amino‐1,4‐enynes.
Authors: Feng Huangdi , Ermolat'ev Denis S. , Song Gonghua , Van der Eycken Erik V. 
Published: July 2012

AbstractThe novel microwave‐promoted cascade approach is air‐ and moisture‐tolerant for the three‐component coupling and opens the way for a selective preparation of highly functionalized synthons.
ChemInform Abstract: Novel Chiral Thiazoline‐Containing N—O Ligands in the Asymmetric Addition of Diethylzinc to Aldehydes: Substituent Effect on the Enantioselectivity.
Authors: Gong Zhiyong , Liu Qingwen , Xue Pengchong , Li Kechang , Song Zhiguang , Liu Zaiqun , Jin Yinghua 
Published: July 2012

AbstractSeveral novel chiral thiazoline ligands of type (I) are synthesized.
ChemInform Abstract: Convenient and Efficient Decarboxylative Aldol Reaction of Malonic Acid Half Esters with Trifluoromethyl Ketones.
Authors: Li Xiao‐Juan , Xiong Heng‐Ying , Hua Ming‐Qing , Nie Jing , Zheng Yan , Ma Jun‐An 
Published: July 2012

AbstractThe process is highly effective with various ketone substrates and the products are formed with yields ≥ 90% within 12‐16h.
ChemInform Abstract: Nitromethane in Polyphosphoric Acid — A New Reagent for Carboxyamidation and Carboxylation of Activated Aromatic Compounds.
Authors: Aksenov Alexander V. , Aksenov Nicolai A. , Nadein Oleg N. , Aksenova Inna V. 
Published: July 2012

AbstractA new method for the relatively easy introduction of carbamoyl and carboxyl groups into activated aromatic compounds is presented.
ChemInform Abstract: Synthesis and Structure of Aroylamidines and N‐Arylbenzamidines Hydrochlorides.
Authors: Kuvaeva E. V. , Fedorova E. V. , Zaitsev V. V. , Yakovlev I. P. , Zakharov V. I. , Semakova T. L. 
Published: July 2012

AbstractArylcarbonitriles (I) react with anhydrous ethanol in the presence of dry hydrogen chloride to the corresponding imidoesters which upon treatment with an alcoholic solution of ammonia yield aroylamidines (II) as salts.
ChemInform Abstract: CuCl‐Catalyzed Stereoselective Conjugate Addition of Grignard Reagents to 2,3‐Allenoates.
Authors: He Jian , Lu Zhan , Chai Guobi , Fu Chunling , Ma Shengming 
Published: July 2012

AbstractIn general Z‐alkenoates are synthesized via Grignard reaction with a high regio‐ and stereoselectivity.
ChemInform Abstract: Poly(ethylene glycol)‐Functionalized Imidazolium Salts—Palladium‐Catalyzed Suzuki Reaction in Water.
Authors: Liu Ning , Liu Chun , Jin Zilin 
Published: July 2012

AbstractA novel PEG‐functionalized imidazolium salt is prepared and successfully used as a water‐soluble ligand in the palladium‐catalyzed Suzuki reaction.
ChemInform Abstract: Novel Bifunctional Organocatalyst Using Sulfamide as Hydrogen Bonding Donor: Application in Asymmetric Michael Addition of Cyclic Ketones to Nitroolefins.
Authors: Deng Fei , Liu Hong‐Ying 
Published: July 2012

AbstractThe PSAP organocatalyst is prepared from Boc‐protected L‐proline in 2 steps.
ChemInform Abstract: Facile Synthesis of Azaspirocycles via Iron Trichloride Promoted Cyclization/Chlorination of Cyclic 8‐Aryl‐5‐aza‐5‐tosyl‐2‐en‐7‐yn‐1‐ols.
Authors: Yeh Ming‐Chang P. , Fang Cheng‐Wei , Lin Hsin‐Hui 
Published: July 2012

AbstractTitle compounds (I) and (III) undergo a very rapid transformation to the desired targets.
ChemInform Abstract: A Clean Synthesis of Novel 3‐Alkoxy‐2‐(benzofuran‐3‐yl)benzofurans via a One‐Pot Pseudo Three‐Component Reaction Using H3PW12O40·xH2O as a Catalyst.
Authors: Jalili‐Baleh Leili , Khoobi Mehdi , Shafiee Abbas , Foroumadi Alireza 
Published: July 2012

AbstractTungstophosphoric acid efficiently catalyzes the one‐pot pseudo three‐component reaction of benzofuran‐3(2H)‐ones and different alcohols to afford the corresponding dimeric products (III) with various alkoxy groups in 3‐position.
ChemInform Abstract: Synthesis of New 1,2,4‐Oxadiazolidin‐5‐ylthiophenes and Thienopyrimidine Derivatives by Aza‐Wittig Reaction Using a Thienyl Carbodiimide.
Authors: Abdel Hameed Afaf M. 
Published: July 2012

AbstractThe aza‐Wittig reaction of iminophosphorane (I) with phenyl isocyanate generates a highly reactive carbodiimide intermediate which undergoes 1,3‐dipolar cycloaddition with nitrone (III) to yield 1,2,4‐oxadiazolium‐5‐ylthiophene (IV).
ChemInform Abstract: Highly Efficient Synthesis of N‐Substituted Isoindolinones and Phthalazinones Using Pt Nanowires as Catalysts.
Authors: Shi Linyan , Hu Lei , Wang Jiaqing , Cao Xueqin , Gu Hongwei 
Published: July 2012

AbstractReductive C—N coupling and intramolecular amidation of 2‐carboxybenzaldehyde with amines is developed as access to a series of isoindolinones where recently reported ultrathin Pt‐nanowires are successfully employed as catalyst.
ChemInform Abstract: Copper‐Catalyzed Approach to Spirocyclic Oxindoles via a Direct C—H, Ar—H Functionalization.
Authors: Moody Catherine L. , Franckevicius Vilius , Drouhin Pauline , Klein Johannes E. M. N. , Taylor Richard J. K. 
Published: July 2012

AbstractConditions A) are suitable for the preparation of various title compounds including precursor (VI) of horsfiline (VII).