bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel enzymatic microreactor with Aspergillus oryzae β‐galactosidase immobilized on silicon dioxide nanosprings
Authors: Schilke Karl F. , Wilson Kelly L. , Cantrell Timothy , Corti Giancarlo , McIlroy David N. , Kelly Christine 
Published: June 2010

AbstractThe use of silicon dioxide (SiO2) nanosprings as supports for immobilized enzymes in a continuous microreactor is described. A nanospring mat (2.2 cm2 × 60 μm thick) was functionalized with γ‐aminopropyltriethoxysilane, then treated with N‐succinimidyl‐3‐(2‐pyridyldithio)‐propionate (SPDP) and dithiothreitol (DTT) to produce surface thiol (SH) groups. SPDP‐modified β‐galactosidase from Aspergillus oryzae was immobilized on the thiolated nanosprings by reversible disulfide linkages. The enzyme‐coated nanospring mat was placed into a 175‐μm high microchannel, with the mat partially occluding the channel. The kinetics and steady‐state conversion of hydrolysis of o‐nitrophenyl β‐D‐galactosylpyranoside at various substrate flow rates and concentrations were measured. Substantial flow was observed through the nanosprings, for which the Darcy permeability κ ≈ 3 × 10−6 cm2. A simple, one‐parameter numerical model coupling Navier‐Stokes and Darcy flow with a pseudo‐first‐order reaction was used to fit the experimental data. Simulated reactor performance was sensitive to changes in κ and the height of the nanospring mat. Permeabilities lower than 10−8 cm2 practically eliminated convective flow through the nanosprings, and substantially decreased conversion. Increasing the height of the mat increased conversion in simulations, but requires more enzymes and could cause sealing issues if grown above channel walls. Preliminary results indicate that in situ regeneration by reduction with DTT and incubation with SPDP‐modified β‐galactosidase is possible. Nanosprings provide high solvent‐accessible surface area with good permeability and mechanical stability, can be patterned into existing microdevices, and are amenable to immobilization of biomolecules. Nanosprings offer a novel and useful support for enzymatic microreactors, biosensors, and lab‐on‐chip devices. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Downstream antibody purification using aqueous two‐phase extraction
Authors: Mao Lisong Nathan , Rogers Jameson K. , Westoby Matthew , Conley Lynn , Pieracci John 
Published: June 2010

AbstractThe extraction of antibodies using a polyethylene glycol (PEG)‐citrate aqueous two‐phase system (ATPS) was investigated. Studies using purified monoclonal antibody (mAb) identified operating ranges for successful phase formation and factors that significantly affected antibody partitioning. The separation of antibody and host cell protein (HCP) from clarified cell culture media was examined using statistical design of experiments (DOE). The partitioning of antibody was nearly complete over the entire range of the operating space examined. A model of the HCP partitioning was generated in which both NaCl and citrate concentrations were identified as significant factors. To achieve the highest purity, the partitioning of HCP from cell culture fluid into the product containing phase was minimized using a Steepest Descent algorithm. An optimal ATPS consisting of 14.0% (w/w) PEG, 8.4% (w/w) citrate, and 7.2% (w/w) NaCl at pH 7.2 resulted in a product yield of 89%, an approximate 7.6‐fold reduction in HCP levels relative to the clarified cell culture fluid before extraction and an overall purity of 70%. A system consisting of 15% (w/w) PEG, 8% (w/w) citrate, and 15% (w/w) NaCl at pH 5.5 reduced product‐related impurities (aggregates and low molecular product fragments) from ∼40% to less than 0.5% while achieving 95% product recovery. At the experimental conditions that were optimized in the batch mode, a scale‐up model for the use of counter‐current extraction technology was developed to identify potential improvements in purity and recovery that could be realized in the continuous operational mode. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Modeling shear‐induced CHO cell damage in a rotary positive displacement pump
Authors: Kamaraju Hari , Wetzel Kenneth , Kelly William J. 
Published: June 2010

AbstractRotary lobe pumps are commonly used in the biotechnology industry for a variety of purposes. Shear damage to animal cells within the rotary lobe pump can adversely affect the product yield or purity during, for example, cell concentration via cross‐flow filtration. In this research, CHO cells grown in 20‐L bioreactors were fed to a rotary lobe pump in both single pass and recycle experiments were conducted at different RPMs and “slip” conditions. The results indicate that the slip flow rate more severely impacts the viability of the CHO cells than the pump RPM. A novel mathematical modeling approach is presented that predicts shear rates in all of the positive displacement pump's slip regions, and then predicts cell death vs. operating conditions. This model accounts for the complex flow situation that results from changes to RPM, backpressure and pump geometry (i.e., clearances). © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
A teaching tool for the state‐of‐the‐art probabilistic methods used in localization of mobile robots
Authors: Farrokhsiar M. , Krys D. , Najjaran H. 
Published: June 2010

AbstractProbabilistic methods provide a powerful paradigm for modeling of robot motion and its environment; a precursor for autonomous navigation of mobile robots. As a graduate‐level engineering course, probabilistic robotics encompasses the techniques used in robot localization and mapping in unstructured environments. This article presents a simulation and animation software program developed mainly as a teaching tool that can help the students visualize different robot localization solutions through both parametric filters (viz., the Extended Kalman Filter, Unscented Kalman Filter) and nonparametric filters (viz., the histogram filter, Rao‐Blackwell particle filter). The program is also a powerful tool for performance analysis and tuning of such filters commonly used for robot localization, mapping, and autonomous navigation. The simulation features dead reckoning (e.g., INS), range‐only sensing (e.g., rangefinder), bearing‐only sensing (e.g., digital camera), or a combination of them. The program includes a simple graphical user interface that allows for changing both filtering and sensing parameters, and monitoring the effects of those changes on the animation of a unicycle in a 2D environment. The program animates the unicycle motion and shows the kinematic results in several graphs simultaneously to evaluate the performance of different methods in finding the robot pose. The program is available as an open‐source Matlab script to facilitate future modifications and improvements of the code by the students interested in robotics, mechatronics, and control engineering. This article presents the features of the program and briefly discusses the algorithms implemented in the software. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 20: 721–727, 2012
Modeling and optimization of a crude distillation unit: A case study for undergraduate students
Authors: García‐Herreros Pablo , Gómez Jorge Mario 
Published: June 2010

AbstractThe need for proper use of process simulators in engineering education is addressed through the modeling and optimization of a crude distillation unit. This exercise has been presented to Chemical Engineering students to help them relate concepts learned in class and the use of software tools widely available in the practice of engineering. The unit, composed of three distillation columns, is simulated using the equilibrium model and the Peng–Robinson equation of state; it is solved using a multiple‐step initialization and the Inside‐Out algorithm. An analysis of the system's degrees of freedom is made in order to specify a base case that is used as the starting point for the optimization. The maximization of the stabilized gasoline flow is achieved through the use of the Sequential Quadratic Programming (SPQ) algorithm. Throughout the exercise, the importance of understanding the mathematical model of the process and the algorithms used is emphasized. The exercise's effectiveness is evaluated by the students considering the achievement of ABET outcomes. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 21: 276–286, 2013
ChemInform Abstract: Asymmetric Synthesis of Boc‐β2‐homophenylglycine.
Authors: PONSINET R. , CHASSAING G. , LAVIELLE S. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Refined Enantioselective Methylation Catalysts: Improved Routes to Bifunctional C5 Synthons.
Authors: JONES G. B. , GUZEL M. , CHAPMAN B. J. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Asymmetric Catalysis. Part 118. Rh(I)‐Catalyzed Asymmetric Hydrosilylation Using New Oxazoline Ligands with Potential Charge‐Transfer Properties.
Authors: BRUNNER H. , STOERIKO R. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Asymmetric Reduction of α,β‐Unsaturated Cyclic Ketones by a Yeast.
Authors: MATSUMOTO K. , KAWABATA Y. , TAKAHASHI J. , FUJITA Y. , HATANAKA M. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Formals and Polyoxymethylene Ethers of N‐Hydroxymethylnitramines.
Authors: ISHCHENKO M. A. , NIKOLAEV V. D. , SOKOLOV A. A. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Facile Synthesis of Spirocyclic Systems Through the Intramolecular Addition of Ketyl Radicals via the Sodium/Ammonia Reduction of δ,ε‐Unsaturated Carboxylic Esters.
Authors: COSSY J. , GILLE B. , BELLOSTA V. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Reactions of Cephalosporin Sulfones. Part 2. Rearrangement of 2α‐Bromocephem Sulfones to Pyrroles.
Authors: GUNDA T. E. , SZOEKE G. N. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: One‐Step Conversion of Oximes to gem‐Chloro‐Nitro Derivatives.
Authors: CECCHERELLI P. , CURINI M. , EPIFANO F. , MARCOTULLIO M. C. , ROSATI O. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Catalytic Liquid‐Phase Oxidation of Cyclooctane.
Authors: CHABUTKINA E. M. , ARTEM'EVA N. V. , KUNITSKII A. A. , ZHUKOV YU. P. , ANTONOVA T. N. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Nuclear Monobromination of Alkyl Phenyl Ethers with NaClO2, NaBr, Mn(acac)3, and Moist Silica Gel in Aprotic Solvent.
Authors: HIRANO M. , MONOBE H. , YAKABE S. , MORIMOTO T. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The Versatile Behavior of the PdCl2/Et3SiH System. Conversion of Alcohols to the Corresponding Halides and Alkanes.
Authors: FERRERI C. , COSTANTINO C. , CHATGILIALOGLU C. , BOUKHERROUB R. , MANUEL G. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Reaction of Fluoro‐Containing β‐Diketones with Ammonium Acetate and Bicarbonate.
Authors: KARPENKO N. S. , FILYAKOVA V. I. , PASHKEVICH K. I. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Reaction of Boron Triflate with Polyfluoroolefins. Synthesis of Polyfluorinated Allyl Trifluoromethanesulfonates.
Authors: PETROV V. A. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and Nitration of N,N′‐Diacylaminals of Aliphatic α‐Nitroaldehydes.
Authors: TERPIGOREV A. N. , GERMANOVA G. V. , BAZANOV A. G. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Ethyl Nitrochlorocyanoacetate in Reactions with Diazomethane and Diazoethane.
Authors: TYRKOV A. G. , AKIMOVA E. YU. , NERETINA E. V. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Diastereoselective Fe(III) Mediated Oxidative Cyclization Reactions of Cyclopropanone Acetals.
Authors: BOOKER‐MILBURN K. I. , BARKER A. , BRAILSFORD W. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Cyclic α‐Amino Acids by Pd‐Mediated Cycloisomerization and Coupling Reactions.
Authors: MOELLER B. , UNDHEIM K. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Azulene‐Annulated Tricyclo[4.3.1.01,6]deca‐2,4,7‐triene Derivatives and Their Anions. A Novel Cycloheptatriene‐norcaradiene Valence Isomerization in the Azulene‐Annulated Tricyclo[4.3.1.01,6]deca‐2,4,7‐triene Ring System.
Authors: NITTA M. , TAKAYASU T. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Chiral Lewis Acid Controlled Diels—Alder Reaction: Factors Affecting the Stereoselective Formation of Both Enantiomers in the Bis(oxazoline)‐magnesium Catalyzed Process.
Authors: CARBONE P. , DESIMONI G. , FAITA G. , FILIPPONE S. , RIGHETTI P. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Contributions to the Chemistry of Bromine Trifluoride. Part 3. Electronic Influences on the Fluorine—Aryl Substitution Reaction of Bromine Trifluoride with Monosubstituted Phenyltrifluorosilanes.
Authors: FROHN H. J. , GIESEN M. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Facile Synthesis of Functionalized Catechols.
Authors: LI C. , ANGELICI R. J. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Palladium‐Catalyzed Carbonylative Cross‐Coupling of Organoboranes with Hypervalent Iodonium Salts: Synthesis of Aromatic Ketones.
Authors: KANG S.‐K. , LIM K.‐H. , HO P.‐S. , YOON S.‐K. , SON H.‐J. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Mixed Organofluorine‐Organosilicon Chemistry. Part 8. One‐Pot Synthesis of 2,2‐Difluoro‐1,5 Diketones from Acylsilanes, Trifluoromethyltrimethylsilane and Enones, and Their Annulation Reaction.
Authors: LEFEBVRE O. , BRIGAUD T. , PORTELLA C. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Efficient Preparation of γ‐Hydroxynitriles via Nitrile Enolate—Epoxide Reactions: Scope and Diastereoselectivity.
Authors: TAYLOR S. K. , DEYOUNG D. , SIMONS L. J. , VYVYAN J. R. , WEMPLE M. A. , WOOD N. K. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: New Homochiral Amino‐Phosphine Ligands: Application in Asymmetric Palladium‐Catalyzed Allylic Alkylation.
Authors: VASCONCELOS I. C. F. , RATH N. P. , SPILLING C. D. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Generation and Intramolecular Cyclization of (2‐Ethenylphenyl)bisketenes. Synthesis of Benzofuranones.
Authors: HEILEMAN M. J. , MOORE H. W. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Simple, Efficient and Convenient Synthesis of Pyrroles and Pyrazoles Using Zeolites.
Authors: SREEKUMAR R. , PADMAKUMAR R. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Expedient Synthesis of N‐Substituted 2‐Aminothiazoles.
Authors: SCHANTL J. G. , LAGOJA I. M. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Tetrazoles. Part 36. Synthesis, Structure and Properties of 5‐Nitrotetrazole.
Authors: KOLDOBSKII G. I. , SOLDATENKO D. S. , GERASIMOVA E. S. , KHOKHRYAKOVA N. R. , SHCHERBININ M. B. , LEBEDEV V. P. , OSTROVSKII V. A. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Novel Facile Method for the Synthesis of 3,4‐Dihydro‐2H‐pyrido[1,2‐a]pyrimidinium Salts.
Authors: KATRITZKY A. R. , QIU G. , YANG B. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.