bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Tachykinins Substance P and Hemokinin-1 Favor the Generation of Human Memory Th17 Cells by Inducing IL-1β, IL-23, and TNF-Like 1A Expression by Monocytes
Authors: Cunin Pierre , Caillon Antoine , Corvaisier Murielle , Garo Erwan , Scotet Mari , Blanchard Simon , Delneste Yves , Jeannin Pascale 
Published: March 2011

Abstract
The nervous system influences immune responses through the release of neural factors such as neuropeptides. Among them, the tachykinin substance P (SP) signals via the neurokinin 1 receptor (NK-1R), which is expressed by various immune cells. We thereby analyzed in this paper whether tachykinins may participate in human CD4+ Th cell polarization. We report that SP and hemokinin-1 (HK-1) upregulate IL-17A and IFN-γ production by human memory CD4+ T cells without affecting IL-4 and IL-10 production. SP and HK-1 switch non–Th17-committed CD4+ memory T cells into bona fide Th17 cells and Th1/Th17 cells. In contrast, SP and HK-1 do not modulate the polarization of naive CD4+ T cells. SP- and HK-1–induced Th17 cell generation is mediated through NK-1R and requires the presence of monocytes. SP and HK-1 trigger IL-1β, IL-6, and TNF-α production, upregulate IL-23 production, and enhance TNF-like 1A expression on monocyte surface. Neutralization experiments demonstrated that IL-1β, IL-23, and TNF-like 1A are involved in the SP- and HK-1–induced Th17 cell. The other members of the tachykinin family, neurokinins A and B, have no effect on the differentiation of naive and memory T cells. These results thereby show that SP and HK-1 are novel Th17 cell-inducing factors that may act locally on memory T cells to amplify inflammatory responses.
Translational Control of NKT Cell Cytokine Production by p38 MAPK
Authors: Nagaleekar Viswas K. , Sabio Guadalupe , Aktan Idil , Chant Alan , Howe Isaac W. , Thornton Tina M. , Benoit Patrick J. , Davis Roger J. , Rincon Mercedes , Boyson Jonathan E. 
Published: March 2011

Abstract
NKT cells are known to rapidly produce a large amount of cytokines upon activation. Although a number of signaling pathways that regulate the development of NKT cells have been identified, the signaling pathways involved in the regulation of NKT cell cytokine production remain unclear. In this study, we show that the p38 MAPK pathway is dispensable for the development of NKT cells. However, NKT cell cytokine production and NKT-mediated liver damage are highly dependent on activation of this pathway. p38 MAPK does not substantially affect cytokine gene expression in NKT cells, but it regulates the synthesis of cytokines through the Mnk–eIF4E pathway. Thus, in addition to gene expression, translational regulation by p38 MAPK could be a novel mechanism that contributes to the overall production of cytokine by NKT cells.
ADAM10 Overexpression Shifts Lympho- and Myelopoiesis by Dysregulating Site 2/Site 3 Cleavage Products of Notch
Authors: Gibb David R. , Saleem Sheinei J. , Kang Dae-Joong , Subler Mark A. , Conrad Daniel H. 
Published: March 2011

Abstract
Although the physiological consequences of Notch signaling in hematopoiesis have been extensively studied, the differential effects of individual notch cleavage products remain to be elucidated. Given that ADAM10 is a critical regulator of Notch and that its deletion is embryonically lethal, we generated mice that overexpress ADAM10 (ADAM10 transgenic [A10Tg]) at early stages of lympho- and myeloid development. Transgene expression resulted in abrogated B cell development, delayed T cell development in the thymus, and unexpected systemic expansion of CD11b+Gr-1+ cells, also known as myeloid-derived suppressor cells. Mixed bone marrow reconstitution assays demonstrated that transgene expression altered hematopoiesis via a cell-intrinsic mechanism. Consistent with previously reported observations, we hypothesized that ADAM10 overexpression dysregulated Notch by uncoupling the highly regulated proteolysis of Notch receptors. This was confirmed using an in vitro model of hematopoiesis via culturing A10Tg hematopoietic Lineage−Sca-1+c-Kit+ cells with OP-9 stromal cells in the presence or absence of Delta-like 1, a primary ligand for Notch. Blockade of the site 2 (S2) and site 3 (S3) cleavage of the Notch receptor demonstrated differential effects on hematopoiesis. OP9-DL1 cultures containing the ADAM10 inhibitor (S2 cleavage site) enhanced and rescued B cell development from wild-type and A10Tg Lineage−Sca-1+c-Kit+ cells, respectively. In contrast, blockade of γ-secretase at the S3 cleavage site induced accumulation of the S2 product and consequently prevented B cell development and resulted in myeloid cell accumulation. Collectively, these findings indicate that the differential cleavage of Notch into S2 and S3 products regulated by ADAM10 is critical to hematopoietic cell-fate determination.
Visual Outcome in Ocular Sarcoidosis: Retrospective Evaluation of Risk Factors
Authors: Miserocchi Elisabetta , Modorati Giulio , Di Matteo Federico , Galli Laura , Rama Paolo , Bandello Francesco 
Published: March 2011

Purpose. To analyze risk factors associated with poor visual outcome in patients with ocular sarcoidosis. Methods. In this retrospective study, charts of 44 patients with uveitis and biopsy-proven sarcoidosis were reviewed. Ocular parameters evaluated were as follows: location, type of uveitis, visual acuity, presence of posterior synechia, iris nodules, vitritis, snowballs, chorioretinal lesions, retinal vasculitis, papillitis, macular edema, cataract, and glaucoma. Final visual acuity of the worst-seeing eye at last follow-up was the outcome considered in univariable and multivariable analyses. Visual acuity of the worst-seeing eye was stratified into 2 categories according to the threshold 20/50 (≤20/50 and >20/50). Results. A total of 44 patients with bilateral uveitis were studied. The majority of patients presented with panuveitis (52%), granulomatous type (61%), posterior synechia (62%). The most frequent vision-threatening complications were cystoid macular edema (56%) and cataract (56%). The median best-corrected visual acuity in the worst-seeing eye at presentation and at end of follow-up was respectively 0.4 (interquartile range [IQR] 0.26–0.80) and 0.63 (IQR 0.36–1.00). At univariable analysis, the presence of iris nodules (p=0.049), cystoid macular edema (p=0.007), and cataract (p=0.007) were clinically significant conditions for a visual outcome of 20/50 or worse in the worst-seeing eye. In multivariable analysis, cystoid macular edema (p=0.034) was the only statistically significant predictor associated with unfavorable visual outcome. Conclusions. In this study, we attempted to find risk factors related to poor visual outcome in patients with ocular sarcoidosis. The results suggest that only the presence of cystoid macular edema was significantly associated with worst visual outcome.
Microstructured thin Peptide-Polymer Films that Spatially Control the Surface-Attachment of Living Cells
Authors: Petersen Sönke , Biesalski Markus 
Published: March 2011

Purpose The integration of living cells into artificial microdevices requires close control of the interfacial interactions. Functional polymer coatings, such as peptide-polymer monolayers, can be used to guide the specific adhesion of living cells on surfaces in a spatially controlled fashion. Methods Silicon surfaces were modified with a protein repellent polymer film composed of poly(dimethylacrylamide) (PDMAA) attached to the peptide cell-recognition motif GRGDSP and a subsequent PDMAA backfill. Microstructuring was achieved through a photolithographical process using surface-bound photoreactive benzophenone. Cell adhesion assays with human fibroblasts were conducted to study the capabilities of this approach to induce a directed outgrowth of living cells and to confine cell colony sizes to single cell arrays. Results Human fibroblasts follow the chemically imprinted microstructures on peptide-polymer coated substrates. Lines of GRGDSP-PDMAA with a width of 10 μm induce a highly elongated cell shape whereas round spots with a diameter of 50 μm support only single cells per spot. Starting at a center-to-center distance of 100 μm between single peptide-polymer spots, cells are able to “bridge” non-adhesive PDMAA areas. Conclusions Peptide-polymer monolayers can direct the outgrowth and restrict the cell colony size through a variation of the imprinted chemical microstructures.
Assemblies of Functional Peptides and Their Applications in Building Blocks for Biosensors
Authors: de la Rica Roberto , Pejoux Christophe , Matsui Hiroshi 
Published: March 2011

AbstractThis feature article highlights our recent applications of functional peptide nanotubes, self‐assembled from short peptides with recognition elements, as building blocks to develop sensors. Peptide nanotubes with high aspect ratios are excellent building blocks for a directed assembly into device configurations, and their combined structures with nanometric diameters and micrometric lengths enables to bridge the “nanoworld” and the “microworld”. When the peptide‐nanotube‐based biosensors, which incorporate molecular recognition units, apply alternating current probes to detect impedance signals, the peptide nanotubes behave as excellent building blocks of the transducer for the detection of target analyes such as pathogens, cells, and heavey metal ions with high specificity. In some sensor configurations, the electric signal can be amplified by coupling them with ion‐specific mineralization via molecular recognition of peptides. In general the detection limit of peptide nanotube chips sensors is very low and the dynamic range of detection can be widened by improved device designs.
Nanocrystalline Magnesium Oxide Stabilized Palladium(0): An Efficient Reusable Catalyst for Room Temperature Selective Aerobic Oxidation of Alcohols
Authors: Layek Keya , Maheswaran H. , Arundhathi R. , Kantam M. Lakshmi , Bhargava Suresh K. 
Published: March 2011

AbstractNanocrystalline magnesium oxide‐stabilized palladium(0) [NAP‐Mg‐Pd(0)], as an efficient catalytic system has been employed for the selective oxidation of alcohols using atmospheric oxygen as a green oxidant at room temperature. Various alcohols could be transformed into their corresponding aldehydes or ketones in good to excellent yields using a set of optimal conditions. NanoActive™ Magnesium Oxide Plus, [NAP‐MgO] with its three‐dimensional structure and well‐defined shape acts as an excellent support for well dispersed palladium(0) nanoparticles. This catalyst can be recovered and reused for several cycles without any significant loss of catalytic activity.
Benzaldehyde Lyase‐Catalyzed Direct Amidation of Aldehydes with Nitroso Compounds
Authors: Ayhan Peruze , Demir Ayhan S. 
Published: March 2011

AbstractBenzaldehyde lyase from the Pseudomonas fluorescens catalyzes the reaction of aromatic aldehydes with nitroso compounds and furnishes N‐arylhydroxamic acids in high yields. Aromatic aldehydes and benzoins are converted into enamine‐carbanion‐like intermediates prior to their reaction with nitroso compounds. The kinetic resolution of rac‐2‐hydroxy‐1,2‐diphenylethanones furnished (S)‐benzoins and arylhydroxamic acids with high enantioselectivities and conversions.
Graphite Oxide as an Auto‐Tandem Oxidation–Hydration–Aldol Coupling Catalyst
Authors: Jia Hong‐Peng , Dreyer Daniel R. , Bielawski Christopher W. 
Published: March 2011

AbstractGraphite oxide (GO) was found to function as an auto‐tandem oxidation–hydration–aldol coupling catalyst for the formation of chalcones in a single reaction vessel. Various alkynes or alcohols were hydrated or oxidizedin situto their corresponding methyl ketones or aldehydes, respectively, which underwent a subsequent Claisen–Schmidt condensation. Each of the aforementioned reactions proceeded in the absence of metals (confirmed by inductively coupled plasma mass spectrometry, ICP‐MS) and afforded a range of chalcone products in good to excellent yields from commercially available starting materials.
Factors associated with working status among workers assessed at a specialized worker's compensation board psychological trauma program
Authors: Hensel Jennifer M. , Bender Ash , Bacchiochi Jason , Dewa Carolyn S. 
Published: March 2011

AbstractBackgroundPsychological morbidity following trauma occurring in the workplace can impact return to work but few studies have investigated this.MethodsThis study was a secondary analysis of administrative data from a specialized workers' compensation board psychological trauma program in Toronto, Canada. Unadjusted and adjusted logistic regression analyses were used to examine factors associated with working status at the time of assessment for workers referred within 1 year of traumatic event.ResultsHaving a disrupted marriage (OR = 3.06, 95% CI 1.14–8.20), sustaining a permanently impairing physical injury (OR = 2.76, 95% CI 1.01–7.55) and the presence of secondary psychiatric diagnoses (OR = 2.55, 95% CI 1.34–4.83) were significantly associated with not working at the time of assessment. When the analyses were subset to workers without permanently impairing physical injuries, only the presence of additional psychiatric diagnoses was significantly associated with not working (OR = 3.81, 95% CI 1.48–9.83).ConclusionsReturn to work after trauma can be a complicated and difficult to treat problem. Social supports, physical rehabilitation and treatment of complex mental health problems likely play a crucial role in improving outcomes. Am. J. Ind. Med. 54:552–559, 2011. © 2011 Wiley‐Liss, Inc.
Effects of lactation on the time‐budgets and foraging patterns of female black howlers (Alouatta pigra)
Authors: Dias Pedro Américo D. , Rangel‐Negrín Ariadna , Canales‐Espinosa Domingo 
Published: March 2011

AbstractLactation is an energy demanding phase in the reproductive cycle of female mammals. For this reason, several studies have assessed the effects of lactation on female behavior. In this study we examine the influence of lactation on the time‐budgets and foraging patterns of female black howlers (Alouatta pigra) in Campeche, Mexico. We observed 32 adult females and 35 infants belonging to 14 groups of black howlers for a total of 2,224 focal hours. We found that lactating females spent more time being inactive and feeding from fruits than nonlactating females. In addition, during the first two‐thirds of lactation females were more active (i.e., rested less, fed more, devoted more time to social activities, and moved more) and foraged more intensively (i.e., ranged over larger distances, used more feeding trees and feeding species, and consumed more leaves) than females in the last third of lactation. Lactation seems to force black howler females to reduce activity and to maximize the intake of high‐quality foods, with inactivity being the highest during late lactation, when females probably face the cumulative effects of nursing older infants and of a new pregnancy. Early lactation is probably the most energetically demanding stage of lactation for black howler females. This study demonstrates that despite being energetically constrained by a highly folivorous diet, reproductive state affects several dimensions of the behavior of black howler females. Therefore, variation in time‐budgets and foraging strategies of howlers has been probably underestimated by previous research that has not considered physiological differences among individuals. Am J Phys Anthropol, 2011. © 2011 Wiley‐Liss, Inc.
Genome‐wide association studies of cerebral white matter lesion burden
Authors: Fornage Myriam , Debette Stephanie , Bis Joshua C. , Schmidt Helena , Ikram M. Arfan , Dufouil Carole , Sigurdsson Sigurdur , Lumley Thomas , DeStefano Anita L. , Fazekas Franz , Vrooman Henri A. , Shibata Dean K. , Maillard Pauline , Zijdenbos Alex , Smith Albert V. , Gudnason Haukur , de Boer Renske , Cushman Mary , Mazoyer Bernard , Heiss Gerardo , Vernooij Meike W. , Enzinger Christian , Glazer Nicole L. , Beiser Alexa , Knopman David S. , Cavalieri Margherita , Niessen Wiro J. , Harris Tamara B. , Petrovic Katja , Lopez Oscar L. , Au Rhoda , Lambert Jean‐Charles , Hofman Albert , Gottesman Rebecca F. , Garcia Melissa , Heckbert Susan R. , Atwood Larry D. , Catellier Diane J. , Uitterlinden Andre G. , Yang Qiong , Smith Nicholas L. , Aspelund Thor , Romero Jose R. , Rice Kenneth , Taylor Kent D. , Nalls Michael A. , Rotter Jerome I. , Sharrett Richey , van Duijn Cornelia M. , Amouyel Philippe , Wolf Philip A. , Gudnason Vilmundur , van der Lugt Aad , Boerwinkle Eric , Psaty Bruce M. , Seshadri Sudha , Tzourio Christophe , Breteler Monique M. B. , Mosley Thomas H. , Schmidt Reinhold , Longstreth W. T. , DeCarli Charles , Launer Lenore J. 
Published: March 2011

AbstractObjective:White matter hyperintensities (WMHs) detectable by magnetic resonance imaging are part of the spectrum of vascular injury associated with aging of the brain and are thought to reflect ischemic damage to the small deep cerebral vessels. WMHs are associated with an increased risk of cognitive and motor dysfunction, dementia, depression, and stroke. Despite a significant heritability, few genetic loci influencing WMH burden have been identified.Methods:We performed a meta‐analysis of genome‐wide association studies (GWASs) for WMH burden in 9,361 stroke‐free individuals of European descent from 7 community‐based cohorts. Significant findings were tested for replication in 3,024 individuals from 2 additional cohorts.Results:We identified 6 novel risk‐associated single nucleotide polymorphisms (SNPs) in 1 locus on chromosome 17q25 encompassing 6 known genes including WBP2, TRIM65, TRIM47, MRPL38, FBF1, and ACOX1. The most significant association was for rs3744028 (pdiscovery = 4.0 × 10−9; preplication = 1.3 × 10−7; pcombined = 4.0 × 10−15). Other SNPs in this region also reaching genome‐wide significance were rs9894383 (p = 5.3 × 10−9), rs11869977 (p = 5.7 × 10−9), rs936393 (p = 6.8 × 10−9), rs3744017 (p = 7.3 × 10−9), and rs1055129 (p = 4.1 × 10−8). Variant alleles at these loci conferred a small increase in WMH burden (4–8% of the overall mean WMH burden in the sample).Interpretation:This large GWAS of WMH burden in community‐based cohorts of individuals of European descent identifies a novel locus on chromosome 17. Further characterization of this locus may provide novel insights into the pathogenesis of cerebral WMH. Ann Neurol 2011;69:928–939
Variability of water uptake studies of biomedical polymers
Authors: Valenzuela Loreto M. , Michniak Bozena , Kohn Joachim 
Published: March 2011

AbstractWater uptake influences many properties of polymers and has been widely studied. In the context of polymeric biomaterials, several publications reported an unusual high variability of analytical results, without further investigating the cause for this phenomenon. Using selected polymers from the library of L‐tyrosine‐derived polyarylates and poly(D,L lactic acid), we showed that nonaged and nonannealed compression molded film samples exhibit the typical large variation in water uptake observed in previous reports. The introduction of an annealing step allows accurate and reproducible water uptake measurements for these polymers. We evaluated the use of 3H‐radiolabeled water for the determination of water uptake, finding that the use of radiolabeled water yields statistically indistinguishable measurements, compared to gravimetric methods, while providing significant advantages in throughput and sensitivity. Using the recommended methods of annealing and 3H‐radiolabled water, the water uptake profiles of 24 polymers of the library of L‐tyrosine‐derived polyarylates are reported. This article addresses experimental concerns related to water uptake studies and may assist other researchers in improving the accuracy of their water uptake results. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Injection molding and injection compression molding of thin‐walled light‐guided plates with V‐grooved microfeatures
Authors: Huang Ming‐Shyan , Chung Chin‐Feng 
Published: March 2011

AbstractIn this study, we investigated the feasibility of injection molding (IM) and injection compression molding (ICM) for fabricating 3.5‐in. light‐guided plates (LGPs). The LGP was 0.4 mm thick with v‐grooved microfeatures (10 μm wide and 5 μm deep). A mold was designed to fabricate LGPs by IM and ICM. Micromachining was used to make the mold insert. The Taguchi method and parametric analysis were applied to examine the effects of the process parameters on the molding quality. The following parameters were considered: barrel temperature, mold temperature, packing pressure, and packing time. Mold temperature in this investigation was in the conventional range. Increasing the barrel temperature and mold temperature generally improved the polymer melt fill in the cavities with microdimensions. The experimental results for the replication of microfeatures by IM and ICM are presented and compared. The height of the v‐grooved microfeatures replicated by ICM was more accurate than those replicated by IM. Additionally, the flatness of the fabricated LGPs showed that ICM was better than IM for thin‐walled molding. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Impregnation of poly(L‐lactide‐ran‐cyclic carbonate) copolymers with useful compounds with supercritical carbon dioxide
Authors: Tsutsumi Chikara , Fukukawa Naohisa , Sakafuji Jun , Oro Kazuyuki , Hata Kazuaki , Nakayama Yuushou , Shiono Takeshi 
Published: March 2011

AbstractIn this article, we cover the development of L‐lactide (L‐LA) random copolymers into which useful compounds, such as repellents and antibacterial agents, were impregnated by high concentration. Outstanding controlled release materials were developed with statistical random copolymers of L‐LA with cyclic carbonate (CC) [2,2‐dimethyltrimethylene carbonate (2,2‐DTMC) or tetramethylene carbonate (TEMC)] with tin 2‐ethyL‐hexanoate as a catalyst at 150°C (2,2‐DTMC) or 120°C (TEMC) for 24 h without solvent. The preparation of improved controlled release materials was performed with useful organic compounds with low boiling points and synthetic L‐LA random copolymers containing CCs as base materials under supercritical carbon dioxide (scCO2). Low‐boiling‐point compounds, such as d‐limonene and hinokitiol, were used. In impregnation experiments with scCO2, the amounts of low‐boiling‐point compounds increased with increasings L‐LA content. The compound content impregnated into poly(L‐lactide‐ran‐cyclic carbonate) [poly(L‐LA‐ran‐CC)] was greater than that of the experiment with poly(L‐lactide‐ran‐ε‐caprolactone) previously studied. When the enzymatic degradation of poly(L‐LA‐ran‐CC) was performed with proteinase K, copolymers with a greater L‐LA content degraded more rapidly than did copolymers with a greater CC content. In a controlled release experiment with poly(L‐lactide‐ran‐2,2‐dimethyltrimethylene carbonate) (76/24) or poly(L‐lactide‐ran‐tetramethylene carbonate) (81/19), the rate of polymer degradation and the rate of impregnated compound release were almost the same. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Retracted: Preparation and characterization of a bacterial cellulose/chitosan composite for potential biomedical application
Authors: Zhijiang Cai , Chengwei Hou , Guang Yang 
Published: March 2011

AbstractThe following article from the Journal of Applied Polymer Science, “Preparation and Characterization of a Bacterial Cellulose/Chitosan Composite for Potential Biomedical Application” by Cai Zhijiang, Hou Chengwei, and Yang Guang, published online on 3 March 2011 in Wiley OnlineLibrary (J. Appl. Polym. Sci. 2011, 121, 1488; http://onlinelibrary.wiley.com/doi/10.1002/app.33661/full), has been retracted by agreement between the authors, the journal's Editor in Chief, Stefano Tonzani, and the publisher, Wiley Periodicals, Inc. The retraction has been agreed due to significant overlap with respect to another article, published in the Journal of Polymer Research (2011, 18, 739) and authored by a subset of the present authors.