bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recent progress on heterologous protein production in methylotrophic yeast systems
Authors: Tsuda Masashi , Nonaka Koichi 
Published: May 2024

AbstractRecombinant protein production technology is widely applied to the manufacture of biologics used as drug substances and industrial proteins such as recombinant enzymes and bioactive proteins. Various heterologous protein production systems have been developed using prokaryotic and eukaryotic hosts. Especially methylotrophic yeast in eukaryotic hosts is suggested to be particularly valuable because such systems have the following advantages: protein secretion into culture broth, eukaryotic quality control systems, a post-translational modification system, rapid growth, and established recombinant DNA tools and technologies such as strong promoters, effective selection markers, and gene knock-in and -out systems. Many methylotrophic yeasts such as the genera Candida, Ogataea, and Komagataella have been studied since methylotrophic yeast was first isolated in 1969. The methanol-consumption-related genes in methylotrophic yeast are strongly and strictly regulated under methanol-containing conditions. The well-regulated gene expression systems under the methanol-inducible gene promoter lead to the potential application of heterologous protein production in methylotrophic yeast. In this review, we describe the recent progress of heterologous protein production technology in methylotrophic yeast and introduce Ogataea minuta as an alternative production host as a substitute for K. phaffii and O. polymorpha.
GPT-4 Turbo with Vision fails to outperform text-only GPT-4 Turbo in the Japan Diagnostic Radiology Board Examination
Authors: Hirano Yuichiro , Hanaoka Shouhei , Nakao Takahiro , Miki Soichiro , Kikuchi Tomohiro , Nakamura Yuta , Nomura Yukihiro , Yoshikawa Takeharu , Abe Osamu 
Published: May 2024

Abstract
Purpose
To assess the performance of GPT-4 Turbo with Vision (GPT-4TV), OpenAI’s latest multimodal large language model, by comparing its ability to process both text and image inputs with that of the text-only GPT-4 Turbo (GPT-4 T) in the context of the Japan Diagnostic Radiology Board Examination (JDRBE).

Materials and methods
The dataset comprised questions from JDRBE 2021 and 2023. A total of six board-certified diagnostic radiologists discussed the questions and provided ground-truth answers by consulting relevant literature as necessary. The following questions were excluded: those lacking associated images, those with no unanimous agreement on answers, and those including images rejected by the OpenAI application programming interface. The inputs for GPT-4TV included both text and images, whereas those for GPT-4 T were entirely text. Both models were deployed on the dataset, and their performance was compared using McNemar’s exact test. The radiological credibility of the responses was assessed by two diagnostic radiologists through the assignment of legitimacy scores on a five-point Likert scale. These scores were subsequently used to compare model performance using Wilcoxon's signed-rank test.

Results
The dataset comprised 139 questions. GPT-4TV correctly answered 62 questions (45%), whereas GPT-4 T correctly answered 57 questions (41%). A statistical analysis found no significant performance difference between the two models (P = 0.44). The GPT-4TV responses received significantly lower legitimacy scores from both radiologists than the GPT-4 T responses.

Conclusion
No significant enhancement in accuracy was observed when using GPT-4TV with image input compared with that of using text-only GPT-4 T for JDRBE questions.
From Parental Behavior to Sexual Function: Recent Advances in Oxytocin Research
Authors: Dale II Joseph , Harberson Mitchell T. , Hill Jennifer W. 
Published: May 2024

Abstract
Purpose of Review
Oxytocin plays many diverse roles in physiological and behavioral processes, including social activity, parental nurturing, stress responses, and sexual function. In this narrative review, we provide an update on the most noteworthy recent findings in this fascinating field.

Recent Findings
The development of techniques such as serial two-photon tomography and fiber photometry have provided a window into oxytocin neuroanatomy and real-time neuronal activity during social interactions. fMRI and complementary mapping techniques offer new insights into oxytocin's influence on brain activity and connectivity. Indeed, oxytocin has recently been found to influence the acquisition of maternal care behaviors and to mediate the influence of social touch on brain development and social interaction. Additionally, oxytocin plays a crucial role in male sexual function, affecting erectile activity and ejaculation, while its role in females remains controversial. Recent studies also highlight oxytocin's interaction with other neuropeptides, such as melanin-concentrating hormone, serotonin, and arginine vasopressin, influencing social and affective behaviors. Finally, an update is provided on the status of clinical trials involving oxytocin as a therapeutic intervention.

Summary
The exploration of oxytocin's complexities and its interplay with other neuropeptides holds promise for targeted treatment in various health and disease contexts. Overall, these findings contribute to the discovery of new and specific pathways to allow therapeutic targeting of oxytocin to treat disorders.
Specific, nondestructive, and durable adhesion primer for polyolefins
Authors: Morgese Giulia , Siegmann Konstantin , Winkler Martin 
Published: May 2024

AbstractGluing polyolefins [e.g., polyethylene (PE) and polypropylene (PP)] results in a very challenging task. The main reason relies on their low surface energy, which reduces the affinity between the polyolefin surface and the chosen adhesive. To tackle this problem, the most commonly used solutions are physical surface treatments, such as plasma, corona, and flame, which introduce hydrophilic moieties on the plastics surface, thus increasing their surface energy. These approaches require special setups, are unspecific, and can induce material degradation. Furthermore, they provide a transient solution, making the storage of pretreated substrates not recommended. In this work, we developed an easy-to-apply primer for durable bonding of adhesives on PE and PP, as robust alternative to physical treatments. Our primer contains a surface-anchoring moiety and an adhesive-binding group to covalently react with the polyolefin substrate and with the glue. As a surface-anchoring moiety, we chose the perfluorophenylazide (PFPA), which is known to undergo a C–H insertion reaction upon UV activation, while as adhesive-binding groups, we selected OH functions, which can covalently react with the most common commercially available glues. When these two features (i.e., PFPA and OH) are combined in a single molecule, the reaction with the substrate does not occur and the molecule is only physisorbed, inducing no adhesion improvement. Chemisorption only occurs with bicomponent formulations, comprising a hydrophobic trifunctional PFPA and a polymer bearing OH and PFPA groups. Those induced improved adhesion on PP compared to the golden standard plasma with polyurethane-based and two-component epoxy adhesives. Storing the coated substrates at room temperature for up to two months did not alter the adhesion performance, thus further ascribing the developed primers as a promising alternative to plasma treatment.
circPDK1 competitively binds miR-4731-5p to mediate GIGYF1 expression and increase paclitaxel sensitivity in non-small cell lung cancer
Authors: Feng YunYin , Zhang TaoLong , Liu Hong 
Published: May 2024

Abstract
Objective
To investigate the action of circPDK1 in paclitaxel (PTX) resistance in non-small cell lung cancer (NSCLC).

Methods
circPDK1, miR-4731-5p, and GIGYF1 levels were determined by RT-qPCR and Western blot. Cell proliferation was detected by CCK-8 and colony formation assay, apoptosis by flow cytometry, invasion by Transwell assay. The targeting relationship between miR-4731-5p and circPDK1 or GIGYF1 was confirmed by dual luciferase reporter gene and RIP assay. A xenograft tumor model was established to determine the role of circPDK1 in PTX resistance.

Results
circPDK1 was overexpressed in PTX-resistant NSCLC, and depleting circPDK1 hampered proliferation and invasion of PTX-resistant cells, activated apoptosis, and improved PTX sensitivity. circPDK1 bound to miR-4731-5p, and increasing miR-4731-5p expression salvaged the effect of circPDK1 depletion on PTX resistance. miR-4731-5p directly targeted GIGYF1, and upregulating GIGYF1 offset the promoting effect of circPDK1 knockdown on PTX sensitivity. NSCLC tumor growth was inhibited and PTX sensitivity improved when circPDK1 was suppressed.

Conclusion
Depleting circPDK1 promotes PTX sensitivity of NSCLC cells via miR-4731-5p/GIGYF1 axis, thereby inhibiting NSCLC pregnancy.
Elevated histone deacetylase 10 expression promotes the progression of clear cell renal cell carcinoma by Notch-1-PTEN signaling axis
Authors: Zheng Bin , Jiang Xue , Liu Yaqing , Cheng Fajuan , Zhang Yiming , Niu Chengtao , Cong Zixiang , Niu Zhihong , He Wei 
Published: May 2024

Abstract
Background
Clear cell renal cell carcinoma (ccRCC), the most common pathological subtype of kidney cancer, accounts for approximately 70% to 80% of all cases. Histone deacetylase 10 (HDAC10) belongs to the HDAC class IIb subgroup, one of the histone deacetylases (HDAC) family. Previous studies suggest that HDAC10 may regulate the development of multiple tumor types. The specific molecular mechanisms employed by HDAC10 in the etiology of ccRCC still need to be discovered.

Methods
The analysis included examining HDAC10 expression levels and their clinical importance within a cohort of inpatients and ccRCC patients documented in the Tumor Genome Atlas (TCGA). Moreover, the biological functions and underlying molecular mechanisms of HDAC10 were investigated.

Results
HDAC10 showed increased expression in ccRCC tumor tissues. Subsequent analysis revealed overexpression of HDAC10 was associated with advanced clinical phenotype and unfavorable prognosis. The absence of HDAC10 significantly decreased ccRCC cell proliferation and migration capabilities. Mechanistic research suggests that HDAC10 may promote RCC development by activating the Notch-1 pathway and downregulating PTEN expression levels.

Conclusion
In summary, HDAC10 can modulate critical biological processes in ccRCC, including proliferation, migration, and apoptosis. Notably, the Notch-1 pathway and PTEN serve as crucial signaling pathways and target genes through which HDAC10 regulates the progression of ccRCC. These findings offer a novel outlook for ccRCC treatment.
The effect of foliar application of plant growth regulators on functional and qualitative characteristics of wheat (Triticum aestivum L.) under salinity and drought stress conditions
Authors: Tajdari Hamid Reza , Soleymani Ali , Montajabi Nosratolah , Naderi Darbaghshahi Mohammad Reza , Javanmard Hamid Reza 
Published: May 2024

AbstractThis study aimed to investigate the effect of salinity and water stress on the physiological and functional characteristics of winter wheat (Triticum aestivum L.) under the foliar application of plant growth regulators (PGRs). The experiment was carried out as a split plot based on a randomized complete block design with three replications in two environments. In each environment, water stress at two irrigation levels (after 90 and 120 mm of pan evaporation) and with two EC of 1.5 and 10 dS/m in the main plots and spraying of PGRs including salicylic acid (SA), gibberellic acid (GA3), and cytokinins (CK) (purine) content with a concentration of 100 ppm and the control treatment (spraying solution with normal water) were placed in subplots. Results indicated that all treatments caused significant increases in functional and qualitative characteristics and yield of Triticum aestivum L. The saline environment and irrigation level after 120 mm of pan evaporation caused a reduction in grain yield in all traits except for seed proline, seed nitrogen content, and seed protein content. Also, the combined foliar application of GA3 + CK + SA increased yield in most traits. The highest RWC of flag leaves was observed in the foliar application of GA3 + SA (3.36 kg/ha) and then in the foliar application of GA3 + SA + CK (57.87 kg/ha). GA3 interacts with PGR spraying to balance another development under saline and non-saline conditions.
STAT3 phosphorylation at Tyr705 affects DRP1 (dynamin-related protein 1) controlled-mitochondrial fission during the development of apoptotic-resistance in pulmonary arterial endothelial cells
Authors: Zhang Han , Chen Li , Li Jiachen , Sun Jiashu , Zhao Qixu , Wang Sheng , Li Gang 
Published: May 2024

Abstract
Background
The apoptosis-resistant pulmonary arterial endothelial cells (PAECs) are known to be major players in the pulmonary remodeling of pulmonary arterial hypertension (PAH) and exhibit an abnormal metabolic profile with mitochondrial dysfunction. Mitochondrial fission has been shown to regulate the apoptosis of several cell types, but this is largely unexplored in the PAECs.

Objective
The roles of mitochondrial fission control by Dynamin related protein-1 (DRP1) in the development of PAECs apoptosis suppression were investigated in present study and the potential mechanisms behind this were furtherly explored.

Methods
The mitochondrial morphology was investigated in PAECs from PAH rats with the pulmonary plexiform lesions, and the relations of it with DRP1 expression and apoptosis were furtherly identified in apoptosis-resistant PAECs induced by hypoxia. PAECs were isolated from rats with severe PAH and from normal subjects, the apoptotic-resistant PAECs were induced by hypoxia. DRP1 gene knockdown was achieved via DRP1-siRNA, DRP1 and STAT3 phosphorylation were blocked using its inhibitors, respectively. Apoptosis was analyzed by flow cytometry, and mitochondrial morphology was investigated by transmission electron microscope and confocal microscopy.

Results
The PAECs isolated from PAH rats with the pulmonary plexiform-like lesions and displayed lower apoptotic rate with increased DRP1 expression and mitochondrial fragmentation. In addition, similar observations were achieved in apoptosis-resistant PAECs induced by hypoxia. Targeting DRP1 using siRNA and pharmacologic blockade prevented the mitochondrial fission and subsequent apoptotic resistance in PAECs under hypoxia. Mechanistically, STAT3 phosphorylation at Tyr705 was shown to be activated in both PAH and hypoxia-treated PAECs, leading to the regulation of DRP1 expression. Of importance, targeting STAT3Tyr705 phosphorylation prevented DRP1 disruption on apoptosis in PAECs under hypoxia.

Conclusions
These data indicated that STAT3 phosphorylation at Tyr705 impacted DRP1-controlled mitochondrial fission during the development of apoptosis-resistance in PAECs, suggesting mitochondrial dynamics may represent a therapeutic target for PAH.
Three-layered location recommendation algorithm using spectral clustering
Authors: Moayedikia Alireza , Jahani Hamed , Rahman Md Anisur , Ong Kok Leong 
Published: May 2024

Abstract
Users utilize Location-Based Social Networks (LBSNs) to check into diverse venues and share their experiences through ratings and comments. However, these platforms typically feature a considerably larger number of locations than users, resulting in a challenge known as insufficient historical data or user-location matrix sparsity. This sparsity arises because not all users can check into all available locations on a given LBSN, such as Yelp. To address this challenge, this paper proposes combining Spectral Clustering with a three-layered location recommendation model to develop a recommender system named LSC, applied to Yelp datasets. LSC leverages various information, including users’ check-in data, demographics, location demographics, and users’ friendship network data, to train the recommender system and generate recommendations. Evaluation of LSC’s performance utilizes the Yelp dataset and several comparison metrics, such as accuracy, RMSE, and F1-score. The results demonstrate that our proposed algorithm delivers reliable and significant performance improvements across various evaluation metrics compared to competing algorithms.
Autistic Women’s Experiences of the Perinatal Period: A Systematic Mixed Methods Review
Authors: Westgate Verity , Sewell Olivia , Caramaschi Doretta , O’Mahen Heather 
Published: May 2024

AbstractThe perinatal period has challenges for autistic women. This review synthesises evidence on the experiences of autistic women during the perinatal period. This mixed methods evidence synthesis followed JBI guidance for mixed methods systematic reviews. The Mixed Methods Appraisal Tool assessed study quality. Thematic analysis was used to synthesise findings. Thirteen studies were included. Themes identified included sensory demands of the perinatal period are frequently overwhelming; experiencing healthcare as an autistic person is challenging; parenting as an autistic mother has difficulties but also rewards; predictability and control are important in labour and birth. Individualised care with reasonable adjustments can make a difference to the perinatal experiences of autistic women. Despite challenges, autistic women also have many strengths as mothers.
Validation and assessment of psychometric properties of the Greek Eating Behaviors Assessment for Obesity (GR-EBA-O)
Authors: Mavrandrea Panagiota , Aloi Matteo , Geraci Matteo , Savva Androula , Gonidakis Fragiskos , Segura-Garcia Cristina 
Published: May 2024

Abstract
Introduction
With increasing morbidity and risk of death, obesity has become a serious health problem largely attributable to difficulties in finding proper treatments for related diseases. Many studies show how detecting abnormal eating behaviors could be useful in developing effective clinical treatments. This study aims at validating the Greek version of the Eating Behaviors Assessment for Obesity (EBA-O).

Method
After a double English/Greek forward/backward translation of the EBA-O, 294 participants completed the Greek version (GR-EBA-O), the Eating Disorder Examination Questionnaire, the Binge Eating Scale, and the Yale Food Addiction Scale. Confirmatory factor analysis (CFA) and construct validity were calculated, and Two-way MANOVA was computed with the factors of GR-EBA-O controlling for sex and BMI categories.

Results
CFA confirmed the second-order five factors (i.e., food addiction, night eating, binge eating, sweet eating, and prandial hyperphagia) structure of the original EBA-O with excellent fit indices. GR-EBA-O factors were highly correlated. The GR-EBA-O subscales were also significantly correlated with the remaining measures, demonstrating good concurrent validity.

Conclusion
The Greek version of the EBA-O has demonstrated sound psychometric properties and appears a reliable and user-friendly tool to identify pathological eating behaviors in obesity.
Level of evidence: V, descriptive research.
Rock fines breakage by flow-induced stresses against drag: geo-energy applications
Authors: Borazjani Sara , Hashemi Abolfazl , Nguyen Cuong , Loi Grace , Russell Thomas , Khazali Nastaran , Yang Yutong , Dang-Le Bryant , Bedrikovetsky Pavel 
Published: May 2024

AbstractThe paper presents a strength-failure mechanism for colloidal detachment by breakage and permeability decline in reservoir rocks. The current theory for permeability decline due to colloidal detachment, including microscale mobilisation mechanisms, mathematical and laboratory modelling, and upscaling to natural reservoirs, is developed only for detrital particles with detachment that occurs against electrostatic attraction. We establish a theory for detachment of widely spread authigenic particles due to breakage of the particle-rock bonds, by integrating beam theory of particle deformation, failure criteria, and creeping flow. Explicit expressions for stress maxima in the beam yield a graphical technique to determine the failure regime. The core-scale model for fines detachment by breakage has a form of maximum retention concentration of the fines, expressing rock capacity to produce breakable fines. This closes the governing system for authigenic fines transport in rocks. Matching of the lab coreflood data by the analytical model for 1D flow exhibits two-population particle behaviour, attributed to simultaneous detachment and migration of authigenic and detrital fines. High agreement between the laboratory and modelling data for 16 corefloods validates the theory. The work is concluded by geo-energy applications to (i) clay breakage in geological faults, (ii) typical reservoir conditions for kaolinite breakage, (iii) well productivity damage due to authigenic fines migration, and (iv) feasibility of fines breakage in various geo-energy extraction technologies.
Cross-jurisdictional Data Transfer in Health Research: Stakeholder Perceptions on the Role of Law
Authors: Chan Hui Yun , Toh Hui Jin , Lysaght Tamra 
Published: May 2024

AbstractLarge data-intensive health research programmes benefit from collaboration amongst researchers who may be located in different institutions and international contexts. However, complexities in navigating privacy frameworks and data protection laws across various jurisdictions pose significant challenges to researchers seeking to share or transfer data outside of institutional boundaries. Research on the awareness of data protection and privacy laws amongst stakeholders is limited. Our qualitative study, drawn from a larger project in Singapore, revealed insights into stakeholders’ perceptions of the role of law in cross-national health data research. Stakeholders in our study demonstrated a range of perceptions regarding the role of data protection law in governing the collection and transfer of health data for research. The main criticisms included inadequate legal protection to data and lack of uniformed data protection standards. Despite these criticisms, participants recognised the importance of data protection law in supporting cross-border data transfers and proposed measures to improve perceived limitations of existing laws. These measures include strengthening existing legal framework, establishing contractual agreements and imposing severe punishments for data misuse.