bluuebunny/crossref_metadata_2025_split huggingface dataset:
Juvenile hormone III produced in male accessory glands of the longhorned beetle, Apriona germari, is transferred to female ovaries during copulation
Authors: Tian Ling , Ji Bao‐zhong , Liu Shu‐wen , Jin Feng , Gao Jie , Li Sheng 
Published: September 2010

AbstractWe report on juvenile hormone (JH) biosynthesis in vitro by male accessory glands (MAGs) in the longhorned beetle, Aprionona germari, accompanied by the transfer of JH from males to females during copulation. JH was extracted from the MAGs and separated by reversed‐phase high‐performance liquid chromatography. JH III was identified as the major JH by gas chromatography–mass spectrometry. A radiochemical assay and a non‐radioactive method were used to measure the in vitro rate of JH biosynthesis by the MAGs. After 4 h of incubation with 3H‐methionine in the medium, the radioactivity in the MAGs substantially increased. In a separate assay, incubation of the MAGs with non‐radioactive methionine for 4 h resulted in a 39% increase in JH III. Seven‐day‐old males were injected with medium 199 containing 3H–methionine and 24 h later they were mated with virgin females. Hemolymph and the MAGs were collected from the mated males and hemolymph, ovaries and eggs were collected from the mated females for assaying radioactive JH. The radioactivity incorporated into JH in the MAGs was transferred to the females during copulation and later transferred into their eggs. Assayed 1 h after copulation, JH III level in the MAGs decreased 42% and the content of JH III in the male hemolymph did not change, whereas the content of JH III in the female hemolymph and ovaries both increased. © 2010 Wiley Periodicals, Inc.
Influence of antihyperuricemic therapy on the clinical and radiographic progression of gout
Authors: Mccarthy Geraldine M. , Barthelemy Carl R. , Veum Judith A. , Wortmann Robert L. 
Published: September 2010

AbstractTen years ago, we studied the clinical and radiographic manifestations of gout in 60 patients and described 3 patterns of disease. To determine the consequences of management over a 10‐year period, we recently reassessed the 39 available patients of this population. We found that although reduced tophaceous deposition on physical examination correlated with normalization of the serum urate concentration, no correlation existed between radiographic changes and mean serum urate concentrations. Progression of gouty changes on radiography reflected progressive deformity on physical examination. We have described the radiographic changes that occurred in a well‐characterized population of subjects with gout over 10 years and determined that serum urate concentrations alone may not provide an effective means of monitoring the progression of tophaceous disease in bone.
Rheumatoid arthritis in women: Incidence rates in group health cooperative, seattle, washington, 1987–1989
Authors: Dugowson Carin E. , Koepsell Thomas D. , Voigt Lynda F. , Bley Linda , Lee Nelson J. , Daling Janet R. 
Published: September 2010

AbstractAs part of a prospective case‐control study of newly diagnosed rheumatoid arthritis (RA) in women, we identified all cases of probable, definite, or classic RA diagnosed in 1987–1989 in 18–64‐year‐old women who were members of a health maintenance organization based in the Seattle, Washington area. Using both the 1958 and the 1987 American Rheumatism Association criteria for the diagnosis of RA and enrollment data from the health maintenance organization, we calculated the incidence by age and diagnostic class. Rates of RA incidence in women increased steadily with age. The incidence of probable, definite, or classic RA ranged from 13.1 per 100,000 person‐years at risk for 18–29‐year‐old women to 82.1 per 100,000 person‐years for 60–64‐year‐old women. The overall incidence rate, age‐adjusted to the 1980 US female population, was 27.9/100,000 person‐years. The overall incidence rate for definite/classic RA, age‐adjusted to the 1980 US female population, was 23.9 per 100,000 person‐years. When compared with adjusted rates of incidence of definite RA in Rochester, Minnesota, in 1950–1974, the incidence rates we found were 44.7% lower. Methodologic differences, changes in diagnostic criteria, and a declining incidence of RA among women over time may all be partial explanations for these results. The possible effects of reproductive factors, including oral contraceptives use, are discussed.
Synovial fluid levels of complement SC5b‐9 and fragment Bb are elevated in patients with rheumatoid arthritis
Authors: Brodeur James P. , Ruddy Shaun , Schwartz Lawrence B. , Moxley George 
Published: September 2010

AbstractTo determine whether complement turnover in synovial fluids of patients with rheumatoid arthritis (RA) reflects activation by the classical or alternative pathway, we used novel immunoassays to measure products of complement activation (the membrane attack complex SC5b‐9 and the cleavage fragments Bb and C4d). Mean synovial fluid levels of SC5b‐9 were more than 8 times higher in RA than in crystal‐induced arthritis (gout and pseudogout) and over 16 times higher than in degenerative joint disease (DJD). Similarly, Bb levels were more than 3 times higher in RA synovial fluids than in crystal‐induced arthritis and over 7 times higher than in DJD. Levels of C4d did not differ among the groups. SC5b‐9 levels correlated with synovial fluid C3 anaphylatoxin (C3a), Bb, and C4d levels (r = 0.81, 0.62, and 0.51, respectively). In patients with RA, synovial fluid SC5b‐9 levels correlated with C3a and Bb (r = 0.6 and 0.56, respectively) but not with C4d. Therefore, novel assays for complement activation indicate that both classical and alternative pathways are involved in complement turnover and that the alternative pathway contributes more to complement activation in RA than in DJD or crystal‐induced arthritis.
Malignant histiocytosis as a fatal complication of systemic lupus erythematosus
Authors: Grau J. M. , Aguilar J. L. , Cid Maria C. , Urbano‐Maarquez A. 
Published: September 2010

AbstractWe present the case of a 26‐year‐old Caucasian woman with systemic lupus erythematosus (SLE) of 9 years duration, mainly involving the kidney. Her clinical course was complicated in the last year by several infectious and noninfectious events, and after pancytopenia, fever, and extremely high levels of lactate dehydrogenase developed, the woman died. Light and electron microscopy of bone marrow, together with immunophenotyping, disclosed malignant histiocytosis, a complication of SLE that has not been reported.
Osteoarthritic changes in canine articular cartilage, subchondral bone, and synovium fifty‐four months after transection of the anterior cruciate ligament
Authors: Brandt Kenneth D. , Myers Stephen L. , Burr David , Albrecht Marjorie 
Published: September 2010

AbstractAnterior cruciate ligament transection (ACLT) in the dog results in osteophyte formation and in morphologic, metabolic, biochemical, and biomechanical changes in the articular cartilage of the unstable knee that mimic those of human osteoarthritis (OA). However, in dogs studied up to 2 years after ACLT, the changes have appeared to be self‐limiting, which has led to the suggestion that this is a model of cartilage damage and repair, rather than of OA. To ascertain whether changes in articular cartilage and subchondral bone of dogs subjected to ACLT lead to progressive changes of OA, we studied 3 dogs for 54 months after ACLT. Arthrotomy was performed in the dogs to visualize and then transect the anterior cruciate ligament. When the dogs were killed, full‐thickness ulceration of the articular cartilage was seen on the medial femoral condyle and tibial plateau of the unstable knee, while cartilage in other regions was thicker than that of the contralateral knee, consistent with hypertrophic cartilage repair.Synovial infiltration by mononuclear cells was not more severe than that seen in dogs killed at earlier intervals after ACLT, although gross fibrotic thickening of the capsule was apparent in each dog. Histomorphometric studies revealed a marked increase in subchondral bone volume and active bone formation. These findings show that the changes that develop in the canine knee joint after ACLT are progressive and are unambiguously those of OA.
Serial magnetic resonance imaging in juvenile dermatomyositis
Authors: Keim David R. , Hernandez Ramiro J. , Sullivan Donita B. 
Published: September 2010

AbstractMagnetic resonance imaging (MRI) was used to follow the course of juvenile dermatomyositis from the onset of disease through resolution of a primary relapse. The signal intensity of the T2‐weighted image of involved muscles was elevated during periods of disease activity, and returned to approximately normal levels with effective suppression of disease activity. T1‐weighted images of involved muscles remained approximately normal despite disease activity.
Effect of the thiol group on experimental gold‐induced autoimmunity
Authors: Tournade Hervé , Guery Jean‐Charles , Pasquier Régine , Vial Marie‐Cécile , Mandet Chantal , Druet Elvira , Dansette Patrick M. , Druet Philippe , Pelletier Lucette 
Published: September 2010

AbstractBrown Norway rats injected with aurothiopro‐panolsulfonate sodium salt develop systemic autoimmunity. The aim of this study was to assess the influence of the sulfur‐containing group in this experimental model of gold‐induced autoimmunity. It was shown that the sulfur‐containing group does not induce autoimmunity of itself, but potentiates the immunotoxic effects of gold.
Erosive arthritis in monoclonal gammopathy of uncertain significance: Report of four cases
Authors: Vitali Claudio , Baglioni Paolo , Vivaldi Ilo , Cacialli Roberta , Tavoni Antonio , Bombardieri Stefano 
Published: September 2010

AbstractWe report 4 case histories in which an erosive arthritis was associated with the presence of a monoclonal gammopathy of uncertain significance. In all 4 cases, the appearance of paraprotein was noted either before or during the development of the arthritis. Two patients had a rather atypical oligoarthritis, while the others had a rheumatoid‐like, symmetric polyarthritis. A synovial amyloid deposit was present in 2 patients, while mild mixed mononuclear infiltrates were the main pathologic finding in the others. In 2 patients, immunohistochemical investigation demonstrated deposits of immunoglobulin‐derived material of the same isotype as the monoclonal component in the synovial tissue.
Bayesian source detection and parameter estimation of a plume model based on sensor network measurements
Authors: Huang Chunfeng , Hsing Tailen , Cressie Noel , Ganguly Auroop R. , Protopopescu Vladimir A. , Rao Nageswara S. 
Published: September 2010

AbstractWe consider a network of sensors that measure the intensities of a complex plume composed of multiple absorption–diffusion source components. We address the problem of estimating the plume parameters, including the spatial and temporal source origins and the parameters of the diffusion model for each source, based on a sequence of sensor measurements. The approach not only leads to multiple‐source detection, but also the characterization and prediction of the combined plume in space and time. The parameter estimation is formulated as a Bayesian inference problem, and the solution is obtained using a Markov chain Monte Carlo algorithm. The approach is applied to a simulation study, which shows that an accurate parameter estimation is achievable. Copyright © 2010 John Wiley & Sons, Ltd.
Composite thin film and electrospun biomaterials for urologic tissue reconstruction
Authors: Kundu Anup K. , Gelman Joel , Tyson Darren R. 
Published: September 2010

AbstractA replacement material for autologous grafts for urinary tract reconstruction would dramatically reduce the complications of surgery for these procedures. However, acellular materials have not proven to work sufficiently well, and cell‐seeded materials are technically challenging and time consuming to generate. An important function of the urinary tract is to prevent urine leakage into the surrounding tissue—a function usually performed by the urothelium. We hypothesize that by providing an impermeable barrier in the acellular graft material, urine leakage would be minimized, as the urothelium forms in vivo. However, since urothelial cells require access to nutrients from the supporting vasculature, the impermeable barrier must degrade over time. Here we present the development of a novel biomaterial composed of the common degradable polymers, poly(ε‐caprolactone) and poly(L‐lactic acid) and generated by electrospinning directly onto spin‐coated thin films. The composite scaffolds with thin films on the luminal surface were compared to their electrospun counterparts and commercially available small intestinal submucosa by surface analysis using scanning electron microscopy and by analysis of permeability to small molecules. In addition, the materials were examined for their ability to support urothelial cell adhesion, proliferation, and multilayered urothelium formation. We provide evidence that these unique composite scaffolds provide significant benefit over commonly used acellular materials in vitro and suggest that they be further examined in vivo. Biotechnol. Bioeng. 2011; 108:207–215. © 2010 Wiley Periodicals, Inc.
Effects of the antimicrobial tylosin on the microbial community structure of an anaerobic sequencing batch reactor
Authors: Shimada Toshio , Li Xu , Zilles Julie L. , Morgenroth Eberhard , Raskin Lutgarde 
Published: September 2010

AbstractThe effects of the antimicrobial tylosin on a methanogenic microbial community were studied in a glucose‐fed laboratory‐scale anaerobic sequencing batch reactor (ASBR) exposed to stepwise increases of tylosin (0, 1.67, and 167 mg/L). The microbial community structure was determined using quantitative fluorescence in situ hybridization (FISH) and phylogenetic analyses of bacterial 16S ribosomal RNA (rRNA) gene clone libraries of biomass samples. During the periods without tylosin addition and with an influent tylosin concentration of 1.67 mg/L, 16S rRNA gene sequences related toSyntrophobacterwere detected and the relative abundance ofMethanosaetaspecies was high. During the highest tylosin dose of 167 mg/L, 16S rRNA gene sequences related toSyntrophobacterspecies were not detected and the relative abundance ofMethanosaetadecreased considerably. Throughout the experimental period, Propionibacteriaceae and high GC Gram‐positive bacteria were present, based on 16S rRNA gene sequences and FISH analyses, respectively. The accumulation of propionate and subsequent reactor failure after long‐term exposure to tylosin are attributed to the direct inhibition of propionate‐oxidizing syntrophic bacteria closely related toSyntrophobacterand the indirect inhibition ofMethanosaetaby high propionate concentrations and low pH. Biotechnol. Bioeng. 2011;108: 296–305. © 2010 Wiley Periodicals, Inc.
Small‐scale and automatable high‐throughput compositional analysis of biomass
Authors: DeMartini Jaclyn D. , Studer Michael H. , Wyman Charles E. 
Published: September 2010

AbstractConventional wet chemistry methods to determine biomass composition are labor‐ and time‐intensive and require larger amounts of biomass (300 mg) than is often available. To overcome these limitations and to support a high‐throughput pretreatment and hydrolysis (HTPH) screening system, this article reports on the development of a downscaled biomass compositional analysis that is based on conventional wet chemistry techniques but is scaled down by a factor of 100 to use significantly less material. The procedure is performed in readily available high‐performance liquid chromatography vials and can be automated to reduce operator input and increase throughput. Comparison of the compositional analyses of three biomasses determined by the downscaled approach to those obtained by conventional methods showed that the downscaled method measured statistically identical carbohydrate compositions as standard procedures and also can provide reasonable estimates of lignin and ash contents. These results demonstrate the validity of the downscaled procedure for measuring biomass composition to enable the calculation of sugar yields and determination of trends in sugar release behavior in HTPH screening studies. Biotechnol. Bioeng. 2011;108: 306–312. © 2010 Wiley Periodicals, Inc.
Scalable expansion of multipotent adult progenitor cells as three‐dimensional cell aggregates
Authors: Subramanian K. , Park Y. , Verfaillie C.M. , Hu W.S. 
Published: September 2010

AbstractMany applications of stem cell technologies require a large quantity of cells for which scalable processes of cell expansion and differentiation are essential. Multipotent adult progenitor cells (MAPCs) are adult stem cells isolated from the bone marrow with extensive self‐renewal and broad differentiation capabilities. MAPCs are typically cultured surface adherent (2D) and at low cell density, making the large surface required for cell expansion a hindrance for many applications. This study demonstrates that MAPCs can be cultivated as aggregates in an undifferentiated state for at least 16 days, as levels of a number of transcripts, including Oct4, remained similar, Oct4 protein was unchanged, and differentiation to neural progenitor, endothelial cell and hepatocyte like cells was retained. Cultivation of these aggregates in stirred bioreactor lead to a 70‐fold expansion in 6 days with final cell densities of close to 106/mL. Importantly, the MAPC aggregates recovered from stirred bioreactors could be differentiated to hepatocyte‐like cells that expressed albumin, alpha‐1‐antitrypsin (AAT), and tyrosine amino transferase (TAT) transcripts and also secreted albumin and urea. This method of scalable expansion combined with differentiation of MAPCs can potentially be used for generating large numbers of MAPC and MAPC‐derived differentiated cells. Biotechnol. Bioeng. 2011;108: 364–375. © 2010 Wiley Periodicals, Inc.
Conserved MicroRNAs in Chinese hamster ovary cell lines
Authors: Johnson Kathryn C. , Jacob Nitya M. , Nissom Peter Morin , Hackl Matthias , Lee Lim Hseuh , Yap Miranda , Hu Wei‐Shou 
Published: September 2010

AbstractMicroRNAs (miRNAs), a class of short (20–24 nt) non‐coding RNAs that direct post‐transcriptional repression of messenger RNAs, increasingly have been shown to play a key role in regulating cellular physiology. We investigated the prevalence of miRNAs in Chinese hamster ovary (CHO) cells by high‐throughput sequencing. Six cDNA libraries of small RNAs from four CHO cell lines were constructed and sequenced by Illumina sequencing. Three hundred fifty distinct miRNA and miRNA* sequences were identified through homology with other species, including mouse, rat, and human. While the majority of the identified miRNAs appear to be expressed ubiquitously, many miRNAs were found to have a wide range of expression levels between cell lines. The identification of these miRNAs will facilitate investigations of their contribution to the hyperproductivity trait. Biotechnol. Bioeng. 2011;108: 475–480. © 2010 Wiley Periodicals, Inc.
A Novel Competitive Class of α‐Glucosidase Inhibitors: (E)‐1‐Phenyl‐3‐(4‐Styrylphenyl)Urea Derivatives
Authors: Kim Jun Young , Lee Ji Won , Kim Young Soo , Lee Yuno , Ryu Young Bae , Kim Songmi , Ryu Hyung Won , Curtis‐Long Marcus J. , Lee Keun Woo , Lee Woo Song , Park Ki Hun 
Published: September 2010

AbstractCompetitive glycosidase inhibitors are generally sugar mimics that are costly and tedious to obtain because they require challenging and elongated chemical synthesis, which must be stereo‐ and regiocontrolled. Here, we show that readily accessible achiral (E)‐1‐phenyl‐3‐(4‐strylphenyl)ureas are potent competitive α‐glucosidase inhibitors. A systematic synthesis study shows that the 1‐phenyl moiety on the urea is critical for ensuring competitive inhibition, and substituents on both terminal phenyl groups contribute to inhibition potency. The most potent inhibitor, compound 12 (IC50=8.4 μM, Ki=3.2 μM), manifested a simple slow‐binding inhibition profile for α‐glucosidase with the kinetic parameters k3=0.005256 μM−1 min−1, k4=0.003024 min−1, and ${K{{{\rm app}\hfill \atop {\rm i}\hfill}}}$=0.5753 μM.
ChemInform Abstract: Electronic Structure of the Hollow‐Cage M8X12 Clusters.
Authors: LOU L. , GUO T. , NORDLANDER P. , SMALLEY R. E. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Ca31Sn20 and Related Compounds: Novel Zintl Phases Containing Dimers and Pentamers of Tin or Lead.
Authors: GANGULI A. K. , GULOY A. M. , LEON‐ESCAMILLA E. A. , CORBETT J. D. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Preparation of Barium Titanate Films at 55 °C by an Electrochemical Method.
Authors: BENDALE P. , VENIGALLA S. , AMBROSE J. R. , VERINK E. D. JUN. , ADAIR J. H. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Sorption of Hydrogen and Kinetics of Hydrogen Evolution on Amorphous Ni‐Sx Electrodes.
Authors: PASEKA I. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The Corrosion Behavior of Sputter‐Deposited Amorphous Chromium‐ Zirconium Alloys in 6 M HCl Solution.
Authors: KIM J. H. , AKIYAMA E. , HABAZAKI H. , KAWASHIMA A. , ASAMI K. , HASHIMOTO K. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Effect of B2O3 and hBN Crystallinity on cBN Synthesis.
Authors: CHOI J.‐Y. , KANG S.‐J. L. , FUKUNAGA O. , PARK J.‐K. , EUN K. Y. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Rapid and Efficient Synthesis of Alkali Metal‐C60 Compounds in Liquid Ammonia.
Authors: BUFFINGER D. R. , ZIEBARTH R. P. , STENGER V. A. , RECCHIA C. , PENNINGTON C. H. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.