bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improved Expression of His6‐Tagged Strictosidine Synthase cDNA for Chemo‐Enzymatic Alkaloid Diversification
Authors: Yang Liuqing , Zou Hongbin , Zhu Huajian , Ruppert Martin , Gong Jingxu , Stöckigt Joachim 
Published: April 2010

AbstractStrictosidine synthase (STR1) catalyzes the stereoselective formation of 3α(S)‐strictosidine from tryptamine and secologanin. Strictosidine is the key intermediate in the biosynthesis of 2,000 plant monoterpenoid indole alkaloids, and it is a key precursor of enzyme‐mediated synthesis of alkaloids. An improved expression system is described which leads to optimized His6‐STR1 synthesis in Escherichia coli. Optimal production of STR1 was achieved by determining the impact of co‐expression of chaperones pG‐Tf2 and pG‐LJE8. The amount and activity of STR1 was doubled in the presence of chaperone pG‐Tf2 alone. His6‐STR1 immobilized on Ni‐NTA can be used for enzymatic synthesis of strictosidines on a preparative scale. With the newly co‐expressed His6‐STR1, novel 3α(S)‐12‐azastrictosidine was obtained by enzymatic catalysis of 7‐azatryptamine and secologanin. The results obtained are of significant importance for application to chemo‐enzymatic approaches leading to diversification of alkaloids with novel improved structures.
Cell‐Cycle Markers and Biosensors
Authors: Kurzawa Laetitia , Morris May C. 
Published: April 2010

AbstractSince the first schematic illustrations of dividing cells, we have come a long way in characterising eukaryotic cells and defining their cell‐cycle status thanks to a number of complementary approaches. Although most of these approaches rely on cell‐fixation procedures to identify molecular components in cell lysates, cultured cells or tissues, the development of GFP technology has enabled visualisation of virtually any fusion protein in cellulo and in vivo, and the exploitation of functional elements with well‐defined spatiotemporal characteristics has enabled the development of genetically encoded fluorescent markers of cell‐cycle phases, thus providing novel means of characterising the status of living cells in real time with high resolution. Together with technological advances in fluorescence chemistry and imaging approaches, the more recent development of fluorescent biosensors has provided direct means of probing cell‐cycle regulators and of studying their dynamics with high spatial and temporal resolution. Here we review classical approaches that rely on cell fixation to characterise the cell‐cycle status and its regulatory enzymes, and we describe the more recent development of cell‐cycle markers based on genetically encoded fusions of fluorescent proteins with characteristic cell‐cycle features, and of fluorescent biosensor technology to probe cell‐cycle regulators in living cells. Biosensors not only provide a means of characterising the behaviour of cell‐cycle regulators in their natural environment, they are also very useful for comparative studies of biological processes in healthy and pathological conditions, and can be further applied to diagnostic approaches to assess the status of a specific target, and to monitor response to therapeutic intervention.
Endovascular stent placement is an acceptable alternative to reoperation in selected infants with residual or recurrent aortic arch obstruction
Authors: Bentham Jamie , Shettihalli Naveen , Orchard Elizabeth , Westaby Stephen , Wilson Neil 
Published: April 2010

AbstractObjective: To describe endovascular stent placement in infants as a technically feasible option in circumstances where surgery is considered less favorable. Background: Endovascular stent placement has become established as a first line therapy for native coarctation of the aorta or recoarctation in older children where stents capable of expansion to adult size can be placed safely. Surgery remains the therapy of choice in infants and young children. The management of aortic arch obstruction in infants is, however, frequently complicated by complex anatomy or clinical condition that may make surgery or further surgery an unattractive option. There is little reported data and the implications thereof of transcatheter stent placement in aortic arch obstruction in infants. Methods: Between August 2004 and November 2009, 11 patients had aortic arch obstruction treated with endovascular stent placement. The median age and weight at first stent placement was 46 days (range 3–399 days) and 4 kg (range 1.4–8 kg), respectively. In 10 patients, surgical intervention preceded transcatheter stent placement. Four had complex aortic arch obstruction and seven had recoarctation. Results: Reduction in peak systolic gradient to <10 mm Hg was achieved in seven of 10 patients with an improvement in aortic artery diameter to >90% of adjacent aorta in all. The diameter of the arch obstruction increased from a median of 1.60 to 4.90 mm (P = 0.001) and the peak systolic gradient from 45 mm Hg to 8 mm Hg (P < 0.0001). Adverse events occurred in two patients one who required further surgical revision and a second who required placement of a second stent. The median follow up was 3.60 years (range 0.4–5.5 years) with two patients having died at 1.34 and 1.42 years poststent placement. Of the nine patients alive, six have since undergone further angioplasty at a median time interval of 0.77 years (range 0.17–2.76 years). Long‐term complications occurred in none. Conclusions: Endovascular stent placement in infants is technically feasible with good results achievable even in small babies. It should be considered as a therapeutic option in complex cases when surgical alternatives are less favorable. © 2010 Wiley‐Liss, Inc.
Stenting of aortic coarctation: Acute, intermediate, and long‐term results of a prospective multi‐institutional registry—Congenital cardiovascular interventional study consortium (CCISC)
Authors: Holzer Ralf , Qureshi Shakeel , Ghasemi Abdolrahim , Vincent Julie , Sievert Horst , Gruenstein Daniel , Weber Howard , Alday Luis , Peirone Alejandro , Zellers Thomas , Cheatham John , Slack Michael , Rome Jonathan 
Published: April 2010

AbstractIntroduction: Since the 1980s, stent implantation has evolved as an important therapeutic strategy for coarctation of the aorta. However, available data is frequently flawed by short follow‐up, lack of adequate follow‐up imaging, and retrospective nature of data collection. Methods: Data was prospectively collected using a multicenter registry congenital cardiovascular interventional study consortium (CCISC). Between 2000 and 2009, 302 patients from 34 centers with a median weight of 58 kg underwent stent implantation for coarctation. Eligible patients (44%) completed intermediate follow‐up (3–18 months) with integrated imaging (cath, CT, MRI), whereas 21% completed long‐term follow‐up (>18–60 months). Procedural success was defined as UL/LL systolic gradient of less than 20 mm Hg, lack of significant recurrent obstruction, and freedom from unplanned repeat intervention. Results: Acute procedural success was 96%. Cumulative intermediate success was 86%, and cumulative long‐term success was 77%. Unplanned repeat interventions were required in 4%, and aortic wall complications were seen in 1% of patients (dissection n = 1 and aneurysm n = 3). Other adverse events (n = 15) occurred mainly acutely and included technical complications such as stent malposition (n = 9). At long‐term follow‐up, 23% of patients continued to have systolic blood pressure above the 95th centile, 9% had an upper‐to‐lower limb blood pressure gradient in excess of 20 mm Hg, and 32% were taking antihypertensive medication. Conclusions: This study documented acute, intermediate, and long‐term outcome data comparable or superior with other surgical or interventional series. However, even with successful initial stent therapy, patients continue to require long‐term follow‐up and have associated long‐term morbidity, relating to aortic wall complications, systemic hypertension, recurrent obstruction as well as need for repeat intervention. © 2010 Wiley‐Liss, Inc.
Chemical Synthesis, Structure Characterization, and Optical Properties of Hollow PbSx–Solid Au Heterodimer Nanostructures
Authors: Huang Shoushuang , Huang Jianmei , Yang Jian , Peng Jun‐Jun , Zhang Qingbo , Peng Feng , Wang Hongjuan , Yu Hao 
Published: April 2010

AbstractHeterodimer nanostructures have attracted extensive attention, owing to an increasing degree of complexity, functionality, and then importance. So far, all the reported ones are built from solid nanoparticles. Herein, nearly monodisperse heterodimer nanostructures are constructed by hollow PbSx and solid Au domains simultaneously through a mild reaction between PbS nanocrystals and the gold species in the presence of dodecylamine. Control experiments clearly reveal the underlying formation mechanism of the hollow PbSx–solid Au heterodimers. The AuIII species in the solution, lead to the etching of PbS nanocrystals and the AuI species facilitate the control of the number of gold domains per nanoparticle. Dodecylamine molecules not only work as a stabilizer in the reaction, but also act as a reducing agent that could greatly affect the morphology of the product. The optical properties of the heterodimers are investigated based on UV/Vis absorption spectroscopy and Raman spectroscopy. This novel heterodimer nanostructure pushes the development of complex nanocrystal‐based architectures forward, and also provides many opportunities for potential applications.
The Nature of Aqueous Solutions: Insights into Multiple Facets of Chemistry and Biochemistry from Freezing‐Point Depressions
Authors: Zavitsas Andreas A. 
Published: April 2010

AbstractContrary to current widely held beliefs, many concentrated aqueous solutions of electrolytes and nonelectrolytes behave ideally. For both, the same simple equation yields mole fractions of water that are equal to the theoretical activities of water. No empirical activity coefficients or ad hoc parameters are needed. Thermodynamic hydration numbers and the number of particles produced per mole of solute are found by searching freezing‐point depression measurements, as if asking the water, “How much available water solvent is left and how many solute particles are there?” The results answer questions currently under debate: Do solutes alter the nature of water outside their immediate surroundings? What is the number of ion pairs formed by various electrolytes and what affects extents of their formation? What are some factors that cause precipitation of proteins, latexes, and so forth from aqueous solutions upon addition of other solutes (Hofmeister series)? Which nonelectrolytes form aggregates in water and what are the implications? Why do different solutes affect viscosity differently? How do ion‐selective channels in cell membranes function at the molecular level?
Stabilization of HHeF by Complexation: Is it a Really Viable Strategy?
Authors: Giordani Maria , Antoniotti Paola , Grandinetti Felice 
Published: April 2010

AbstractAb initio calculations at the MP2 and CCSD(T) levels of theory have disclosed the conceivable existence of fluorine‐coordinated complexes of HHeF with alkali‐metal ions and molecules M+(M+=Li+–Cs+), M+–OH2, M+–NH3(M+=Li+, Na+), and MX (M=Li, Na; X=F, Cl, Br). All these ligands L induce a shortening of the HHe distance and a lengthening of the HeF distance accompanied by consistent blue‐ and redshifts, respectively, of the HHe and HeF stretching modes. These structural effects are qualitatively similar to those predicted for other investigated complexes of the noble gas hydrides HNgY, but are quantitatively more pronounced. For example, the blueshifts of the HHe stretching mode are exceptionally large, ranging between around 750 and 1000 cm−1. The interactions of HHeF with the ligands investigated herein also enhance the (HHe)+F−dipole character and produce large complexation energies of around 20–60 kcal mol−1. Most of the HHeF–L complexes are indeed so stable that the three‐body dissociation of HHeF into H+He+F, exothermic by around 25–30 kcal mol−1, becomes endothermic. This effect is, however, accompanied by a strong decrease in the HHeF bending barrier. The complexation energies, ΔE, and the bending barriers,E*, are, in particular, related by the inverse relationshipE*(kcal mol−1)=6.9exp[−0.041ΔE(kcal mol−1)]. Therefore the HHeFL complexes, which are definitely stable with respect to H+He+F+L (ΔE≈25–30 kcal mol−1), are predicted to have bending barriers of only 0.5–2 kcal mol−1. Overall, our calculations cast doubt on the conceivable stabilization of HHeF by complexation.
Direct Electrochemistry of Cytochrome c at Modified Si(100) Electrodes
Authors: Ciampi Simone , Gooding J. Justin 
Published: April 2010

AbstractThis paper demonstrates the direct electron transfer between the heme moiety of horse hearth cytochrome c and a pyridinyl group on self‐assembled‐monolayer‐modified Si(100) electrodes. Self‐assembled monolayers (SAMs) containing the putative receptor ligand were prepared by a step‐wise procedure using “click” reactions of acetylene‐terminated alkyl monolayers and isonicotinic acid azide derivatives. Unoxidized Si(100) electrodes, possessing either isonicotinate or isonicotinamide receptor ligands, were characterized using X‐ray photoelectron spectroscopy, contact‐angle goniometry, cyclic voltammetry, and electrochemical impedance spectroscopy. The ability of isonicotinic acid terminated layers to coordinatively bind the redox center of cytochrome c was found to be restricted to pyridinyl assemblies with a para‐ester linkage present. The protocol detailed here offers an experimentally simple modular approach to producing chemically well‐defined SAMs on silicon surfaces for direct electrochemistry of a well‐studied model redox protein.
BCl3‐Mediated Ene Reaction of Sulfur Dioxide and Unfunctionalized Alkenes
Authors: Marković Dean , Volla Chandra M. R. , Vogel Pierre , Varela‐Álvarez Adrián , Sordo José A. 
Published: April 2010

AbstractThe first ene reactions of SO2 and unfunctionalized alkenes are reported. Calculations suggest that the endergonic ene reactions of SO2 with alkenes can be used to generate β,γ‐unsaturated sulfinyl and sulfonyl compounds. Indeed, in the presence of one equivalent of BCl3, the unstable sulfinic acid form stable sulfinic acid⋅BCl3 complexes that can be reacted in situ with NCS to generate corresponding sulfonyl chlorides, or with a base to generate corresponding sulfinates. The latter can be reacted with electrophiles to generate sulfones, or with silyl chloride to form β,γ‐unsaturated silyl sulfinates. The sulfinic acid⋅BCl3 complexes can be reacted with ethers that act as oxygen nucleophiles to produce corresponding sulfinic esters. Thus one‐pot, three‐component synthesis of β,γ‐unsaturated sulfonamides, sulfinyl esters and sulfones have been developed starting from alkenes and sulfur dioxide (reagent and solvent).
Rapid Colorimetric Identification and Targeted Photothermal Lysis of Salmonella Bacteria by Using Bioconjugated Oval‐Shaped Gold Nanoparticles
Authors: Wang Shuguang , Singh Anant K. , Senapati Dulal , Neely Adria , Yu Hongtao , Ray Paresh C. 
Published: April 2010

AbstractSalmonella bacteria are the major cause for the infection of 16 million people worldwide with typhoid fever each year. Antibiotic‐resistant Salmonella strains have been isolated from various food products. As a result, the development of ultrasensitive sensing technology for detection and new approaches for the treatment of infectious bacterial pathogens that do not rely on traditional therapeutic regimes is very urgent for public health, food safety, and the world economy. Driven by this need, we report herein a nanotechnology‐driven approach that uses antibody‐conjugated oval‐shaped gold nanoparticles to selectively target and destroy pathogenic bacteria. Our experiments have shown the use of a simple colorimetric assay, with an anti‐salmonella antibody conjugated to oval‐shaped gold nanoparticles, for the label‐free detection of S. typhimurium with an excellent detection limit (104 bacteria per mL) and high selectivity over other pathogens. When bacteria conjugated to oval‐shaped gold nanoparticles were exposed to near‐infrared radiation, a highly significant reduction in bacterial cell viability was observed due to photothermal lysis. Ideally, this nanotechnology‐based assay would have enormous potential for rapid, on‐site pathogen detection to avoid the distribution of contaminated foods.
Experimental Evidence for the Involvement of Dinuclear Alkynylcopper(I) Complexes in Alkyne–Azide Chemistry
Authors: Buckley Benjamin R. , Dann Sandra E. , Heaney Harry 
Published: April 2010

AbstractDinuclear alkynylcopper(I) ladderane complexes are prepared by a robust and simple protocol involving the reduction of Cu2(OH)3OAc or Cu(OAc)2 by easily oxidised alcohols in the presence of terminal alkynes; they function as efficient catalysts in copper‐catalysed alkyne–azide cycloaddition reactions as predicted by the Ahlquist–Fokin calculations. The same copper(I) catalysts are formed during reactions by using the Sharpless–Fokin protocol. The experimental results also provide evidence that sodium ascorbate functions as a base to deprotonate terminal alkynes and additionally give a convincing alternative explanation for the fact that the CuI‐catalysed reactions of certain 1,3‐diazides with phenylacetylene give bis(triazoles) as the major products. The same dinuclear alkynylcopper(I) complexes also function as catalysts in cycloaddition reactions of azides with 1‐iodoalkynes.
ChemInform Abstract: Crystal Growth, Crystal Structure of New Polymorphic Modification, β‐Bi2B8O15 and Thermal Expansion of α‐Bi2B8O15.
Authors: Bubnova R. S. , Alexandrova J. V. , Krivovichev S. V. , Filatov S. K. , Egorysheva A. V. 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Phase Relationships of the La—Al—Sb System at 773 K from 0 to 50.0 at.% Sb.
Authors: Chen Yeqing , Feng Qiang , Zeng Lingmin , He Jiejun , He Wei 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and Structural Characterization of a New Two‐Dimensional Organic‐Inorganic Hybrid Molybdoarsenate: [Cu(en)2]2 [(CuO6)Mo6O18 (As3O3)2].
Authors: Wu Qiang , Han Qiuxia , Chen Lijun , Ma Pengtao , Niu Jingyang 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Crystal Growth, Structure and Magnetic Properties of the Double Perovskites Ln2MgIrO6 (Ln: Pr, Nd, Sm—Gd).
Authors: Mugavero Samuel J. III , Fox Adam H. , Smith Mark D. , zur Loye Hans‐Conrad 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Palladium‐Catalyzed Aryl C—H Bonds Activation/Acetoxylation Utilizing a Bidentate System.
Authors: Gou Fa‐Rong , Wang Xiang‐Chuan , Huo Peng‐Fei , Bi Hai‐Peng , Guan Zheng‐Hui , Liang Yong‐Min 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Nickel‐Catalyzed Regioselective Carbomagnesation of Methylenecyclopropanes Through a Site‐Selective Carbon—Carbon Bond Cleavage.
Authors: Terao Jun , Tomita Masahiro , Singh Surya Prakash , Kambe Nobuaki 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Ruthenium‐Catalyzed Selective Regio‐ and Mono‐Allylation of Cyclic 1,3‐Diketones Using Allyl Alcohols as Substrates.
Authors: Gruber Stefan , Pregosin Paul S. 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Stereoselective Phosphine‐Catalyzed Synthesis of Highly Functionalized Diquinanes.
Authors: Wilson Jonathan E. , Sun Jianwei , Fu Gregory C. 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Convenient, Highly Stereoselective, Metal‐Free Synthesis of Chiral Amines.
Authors: Guizzetti Stefania , Benaglia Maurizio , Biaggi Cinzia , Celentano Giuseppe 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Pd/C‐Catalyzed Reductive Mono‐N‐alkylation of Nitrophenol Derivatives in One‐Pot Way.
Authors: Jin Zhonghao , Li Dao , Wang Xingyi , Lu Guanzhong 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: An Efficient MnCl2‐Catalyzed Tandem Acylation‐Cross‐Coupling Reaction of o‐Halobenzoyl Chloride with Diorganyl Magnesium Compounds.
Authors: Zhang Fengmin , Shi Zhuangzhi , Chen Feng , Yuan Yu 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Ruthenium‐Catalyzed Synthesis of β‐Oxo Esters in Aqueous Medium: Scope and Limitations.
Authors: Cadierno Victorio , Francos Javier , Gimeno Jose 
Published: April 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.