bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Integrated Atmospheric Microwave Plasma Source
Authors: Kovacs Reinhold , Bibinov Nikita , Awakowicz Peter , Porteanu Horia‐Eugen , Kühn Silvio , Gesche Roland 
Published: August 2009

AbstractAtmospheric plasma processes become more and more popular in recent times. A new integrated atmospheric plasma source is presented which consists of a microwave resonator combined with a solid‐state power oscillator. This allows for a very compact and efficient design of a microwave plasma source without external microwave power supply and matching units. Hydrophobic polymers have to be activated to ensure an effective painting or glueing. The performance of this new plasma source has been investigated with respect to surface activation depending on axial and radial distance to the substrate, process time, process gas, and flow velocity. Several polymeric materials have been compared. Polyethylene, polyamide, polystyrene, polypropylene, polycarbonate, and polytetrafluorineethylene show good activation results. This tool can be used especially for bulky goods and/or mass products, when a vacuum process is not possible or too expensive.
Adhesion Improvement of Polyimide/Metal Interface by He/O2/NF3 Atmospheric Pressure Plasma
Authors: Lee Su Bin , Kim Yoon‐Kee 
Published: August 2009

AbstractThe surface of polyimide (PI) was modified with He/O2/NF3 dielectric barrier discharge generated by 13.56 MHz r.f. at atmospheric pressure in order to improve adhesion between metal (Cu/Cr) and PI. He/O2/NF3 plasma severely etched the surface of PI and formed many conical clusters of below 100 nm diameter on the PI surface. The surface roughness of PI was increased from 0.4 to 11 nm with r.f. power. X‐ray photoelectron spectroscopy (XPS) analysis showed that oxygen concentration was increased as much as the decrease of carbon concentration after plasma treatment. XPS analysis also showed that the component ratio of CO bonding, which is a hydrophilic functional group, was increased about 30% by the plasma treatment as compared with untreated PI. T‐peel test showed that peel strength was increased with r.f. power. The highest peel strength was 148 gf · nm−1, which was about 50 times as high as the peel strength obtained without plasma activation. It is more than two times as strong as the adhesion between the Cu/Cr film and PI substrate treated by O2 plasma. The surface morphology and chemical composition of the detached side of the PI treated by He/O2/NF3 plasma were similar to untreated PI.
Mechanical Study of High Resistance Silicon Carbide Based Multi‐Nano‐Layers Grown by Multifrequency PACVD
Authors: Rebib Farida , Gaudy Thomas , Soum‐Glaude Audrey , Caron Isabelle , Da Silva Tony , Picard Christine , Cournut Fabienne , Thomas Laurent 
Published: August 2009

AbstractTitanium alloys are commonly used to lighten aeronautical structures. Unfortunately, their poor tribological properties need to confer onto the surface both high mechanical resistance under load and low friction/low wear against other metals. Based on amorphous silicon carbide (a‐SiC:H), periodic multi‐nano‐coatings have been found to be candidates for this use. Such coatings were deposited on Ti surfaces using multifrequency plasma CVD devices. Scratch‐testing measurements have revealed that the critical loads corresponding to cohesive/adhesive cracks are significantly increased using multilayered stacks. Fretting‐wear tests also revealed the influence of multilayer architecture and preliminary analysis of wear tracks and material transfers leaded to a first classification of the coatings.
Impact of incomplete DNase I treatment on human macrophage proteome analysis
Authors: Acosta‐Martin Adelina Elena , Chwastyniak Maggy , Beseme Olivia , Drobecq Hervé , Amouyel Philippe , Pinet Florence 
Published: August 2009

AbstractThe aim of our study was to analyze the proteomic pattern of human macrophages obtained over a 4 year period from blood donors. The purpose was to simulate a long‐term clinical study to assess the application of 2‐D DIGE technique for differential proteomic analysis of these scarce samples. Bioinformatic analysis of 2‐D DIGE gels of 19 different cultures of macrophages assessed whether they did or did not contain at least specific five spots identified by MS as being or containing bovine deoxyribonuclease I (DNase I). Bovine DNase I was used during sample treatment to remove nucleic acids from protein extracts. Macrophages were classified in two groups, which appeared to be differentiated by the completeness of DNase I treatment. Further detailed analysis revealed a different proteomic pattern of macrophage protein samples according to the completeness of this treatment. The major group of proteins affected, accounting for one third of the differentially expressed proteins, included proteins involved in cell motion and actin cytoskeleton reorganization. The use of DNase I for the removal of nucleic acids from protein samples must be avoided in proteomic studies since it can generate bias in the analysis of protein expression patterns.
Optimization of the Synthesis of 2,4,6,8,10,12‐Hexaallyl‐ 2,4,6,8,10,12‐Hexaazaisowurtzitane
Authors: Adamiak Joanna , Maksimowski Paweł 
Published: August 2009

Abstract2,4,6,8,10,12‐Hexaallyl‐2,4,6,8,10,12‐hexaazaisowurtzitane (HALLIW) was obtained in a condensation reaction of glyoxal with allylamine in the presence of a protonic acid as a catalyst. Optimization of the synthesis was accomplished by means of a mathematical experiment planning theory with the steepest descent method. The effect of the following parameters was examined: process temperature, amount of the catalyst, amount of the solvent, glyoxal addition time, reaction time, and the ratio of the substrates. As a result of the study an improved yield was achieved from 20 to 66.5%. The highest yield for the reaction was found for the conditions (mole ratios relative to glyoxal): catalyst (formic acid) 0.1 : 1; solvents: acetonitrile 9.35 : 1, water 0.18 : 1; allylamine 2.2 : 1; temperature 15 °C; glyoxal addition time 15 min, reaction time 60 min. The studies were conducted on a small laboratory scale where the yield and purity of the product obtained were examined. A method was developed for purification of HALLIW. NMR, IR spectroscopy, DSC, and TG analyses were employed in the study and their results are reported.
Effects of acute and subacute garlic supplement administration on serum total antioxidant capacity and lipid parameters in healthy volunteers
Authors: Koseoglu M. , Isleten F. , Atay A. , Kaplan Y. C. 
Published: August 2009

AbstractGarlic has long been used for medicinal purposes. It has been shown that different forms of garlic have significant antioxidant effects. The strong flavor, odor and unwanted gastrointestinal side effects of fresh garlic has rendered the use of commercial garlic supplements as a preferable option. To investigate the effects of garlic supplementation on serum total antioxidant capacity and lipid parameters, 17 healthy volunteers were administered four standardized commercial garlic tablets every day for 30 days. Blood samples were taken at day 1 (before the first administration of tablets [control] and at 3 h after the administration of tablets), 15 and 30 days, respectively. Total antioxidant capacity (TAC), total cholesterol, low density lipoprotein cholesterol (LDL cholesterol), high density lipoprotein cholesterol (HDL cholesterol) and triglyceride (TG) were measured in sera. Serum TAC was increased significantly at 30 days compared with 15 days, 3 h and control. There was also a significant increase in serum TAC at 15 days compared with 3 h and control. Total cholesterol, LDL cholesterol, HDL cholesterol and TG were not found to be significantly different between control, 3 h, 15 and 30 days. These data suggest that garlic, used as a dietary supplementation, may be beneficial in increasing the antioxidant capacity of the body. Copyright © 2009 John Wiley & Sons, Ltd.
Citrus bergamiarisso & poiteau juice protects against renal injury of diet‐induced hypercholesterolemia in rats
Authors: Trovato Ada , Taviano Maria F. , Pergolizzi Simona , Campolo Loredana , De Pasquale Rita , Miceli Natalizia 
Published: August 2009

AbstractThe present study was designed to evaluate the protective effect of treatment withCitrus bergamiajuice (1 mL/day, for 30 days) against hypercholesterolemic diet‐induced renal injury in rat.C. bergamiajuice provoked a significant reduction in the plasma levels of cholesterol, triglycerides and LDL, and an increase in HDL levels, versus hyperlipidemic controls (p< 0.05). Plasma creatinine levels, measured to assess renal glomerular function, did not change compared with hyperlipidemic controls (0.37 ± 0.11 mg/dL and 0.32 ± 0.10 mg/dL, respectively).Moreover,in vivolipid peroxidation was measured in kidney homogenate;C. bergamiajuice administration significantly decreased MDA levels elevations compared with hyperlipidemic controls (4.10 ± 0.10 nmol/mg protein and 4.78 ± 0.15 nmol/mg protein, respectively).Histological observations of the kidney supported the biochemical data and indicated a protective effect ofC. bergamiajuice on the development of renal damage in hypercholesterolemic rats.The antioxidant potential ofC. bergamiajuice was examined in twoin vitrosystems: in the DPPH test the juice showed a noticeable effect on scavenging free radicals (IC50= 25.01 ± 0.70 ±L); in the reducing power assay it showed a strong activity, too (1.44 ± 0.01 ASE/mL).These findings suggest thatC. bergamiajuice has a protective role in hypercholesterolemic diet‐induced renal damage, which may be attributed to its antioxidant properties. Copyright © 2009 John Wiley & Sons, Ltd.
Antiinflammatory effects of a red orange extract in human keratinocytes treated with interferon‐gamma and histamine
Authors: Cardile Venera , Frasca Giuseppina , Rizza Luisa , Rapisarda Paolo , Bonina Francesco 
Published: August 2009

AbstractRed oranges are an important component of the so‐called Mediterranean diet and they have been used by traditional medicine for their health protective properties, particularly to heal sore throat and cough, suggesting an interesting antiinflammatory activity. The purpose of this study was to evaluate the antiinflammatory activity of a red orange (Citrus sinensis varieties: Moro, Tarocco, Sanguinello) complex (ROC), characterized by high levels of anthocyanins, flavanones, hydroxycinnamic acids and ascorbic acid, on the human keratinocyte line NCTC 2544 exposed to interferon‐gamma (IFN‐γ) and histamine. The expression of immunomodulatory membrane molecules such as inter‐cellular adhesion molecule‐1 (ICAM‐1) by Western blot analysis, and the release of chemokines such as monocyte chemoattractant protein‐1 (MCP‐1) and interleukin‐8 (IL‐8) through ELISA kits, were determined. ICAM‐1 modulates the permanence and activation of T lymphocytes in the epidermis. MCP‐1 is a specific chemoattractant for monocytes and dendritic cells. IL‐8 is important for the recruitment of both neutrophils and T lymphocytes. Addition of ROC at different concentrations together with IFN‐γ and histamine induced a dose‐dependent inhibition of ICAM‐1 expression and MCP‐1 and IL‐8 release. ROC shows interesting antiinflammatory properties in human keratinocyte cells NCTC 2544. This natural complex could have a topical employment and mitigate the consequences of some skin pathologies. Copyright © 2009 John Wiley & Sons, Ltd.
Reciprocal regulation of gene expression by Ephedra herba in mouse brain
Authors: Kim Bu‐Yeo , Cao Li Hua , Park Hwayong , Jeong Sangkyun 
Published: August 2009

AbstractAlthough Ephedra herba has been used to treat a variety of diseases, the molecular mechanism has not been defined clearly. To identify the molecular events, a microarray analysis was conducted using the brain tissue of mice orally administered aqueous extracts of Ephedra herba, in which the expression level of many genes was altered depending on the duration of treatment time. In particular, temporal changes in the expressions of genes can be classified into two major reciprocal patterns in which 175 and 282 genes were included in pattern 1 and 2, respectively. Classification based on gene ontology revealed different functional implications of genes between temporal pattern 1 and 2. Through promoter analysis of temporally co‐expressed genes, putative co‐regulated genes could be segregated based on the similarity of the transcription factor binding sites (TFBS). Interestingly, two temporal patterns were associated with different sub‐classes of TFBS similarity, which implies the presence of dual regulatory mechanisms. Finally, through interaction analysis, core node genes that could interact with many other nodes were identified. By integrating the interaction information with promoter clustering, temporally co‐regulated key components could be selected in mouse brain after administration of Ephedra herba. Copyright © 2009 John Wiley & Sons, Ltd.
Asymptotic confidence intervals for variograms of stationary time series
Authors: Bisgaard Søren , Khachatryan Davit 
Published: August 2009

AbstractIndustrial processes are often monitored via data sampled at a high frequency and hence are likely to be autocorrelated time series that may or may not be stationary. To determine if a time series is stationary or not the standard approach is to check whether sample autocorrelation function fades out relatively quickly. An alternative and somewhat sounder approach is to use the variogram. In this article we review the basic properties of the variogram and then derive a general expression for asymptotic confidence intervals for variogram based on the Delta method. We illustrate the computations with an industrial process example. Copyright © 2009 John Wiley & Sons, Ltd.
Heterobifunctional isotope‐labeled amine‐reactive photo‐cross‐linker for structural investigation of proteins by matrix‐assisted laser desorption/ionization tandem time‐of‐flight and electrospray ionization LTQ‐Orbitrap mass spectrometry
Authors: Krauth Fabian , Ihling Christian H. , Rüttinger Hans Hermann , Sinz Andrea 
Published: August 2009

AbstractChemical cross‐linking combined with a subsequent enzymatic digestion and mass spectrometric analysis of the created cross‐linked products presents an alternative approach to assess low‐resolution protein structures. By covalently connecting pairs of functional groups within a protein or a protein complex a set of structurally defined interactions is built up. We synthesized the heterobifunctional amine‐reactive photo‐cross‐linker N‐succinimidyl p‐benzoyldihydrocinnamate as a non‐deuterated (SBC) and doubly deuterated derivative (SBDC). Applying a 1:1 mixture of SBC and SBDC for cross‐linking experiments aided the identification of cross‐linked amino acids in the mass spectra based on the characteristic isotope patterns of fragment ions. The cross‐linker was applied to the calcium‐binding protein calmodulin with a subsequent analysis of cross‐linked products by nano‐high‐performance liquid chromatography matrix‐assisted laser desorption/ionization tandem time‐of‐flight mass spectrometry (nano‐HPLC/MALDI‐TOF/TOF‐MS) and nano‐HPLC/nano‐electrospray ionization (ESI)‐LTQ‐Orbitrap‐MS. Copyright © 2009 John Wiley & Sons, Ltd.
Efficient production of L‐lactic acid by Crabtree‐negative yeast Candida boidinii
Authors: Osawa Fumi , Fujii Toshio , Nishida Takehisa , Tada Nobuki , Ohnishi Toru , Kobayashi Osamu , Komeda Toshihiro , Yoshida Satoshi 
Published: August 2009

AbstractIndustrial production of L‐lactic acid, which in polymerized form as poly‐lactic acid is widely used as a biodegradable plastic, has been attracting world‐wide attention. By genetic engineering we constructed a strain of the Crabtree‐negative yeast Candida boidinii that efficiently produced a large amount of L‐lactic acid. The alcohol fermentation pathway of C. boidinii was altered by disruption of the PDC1 gene encoding pyruvate decarboxylase, resulting in an ethanol production that was reduced to 17% of the wild‐type strain. The alcohol fermentation pathway of the PDC1 deletion strain was then successfully utilized for the synthesis of L‐lactic acid by placing the bovine L‐lactate dehydrogenase‐encoding gene under the control of the PDC1 promoter by targeted integration. Optimizing the conditions for batch culture in a 5 l jar‐fermenter resulted in an L‐lactic acid production reaching 85.9 g/l within 48 h. This productivity (1.79 g/l/h) is the highest thus far reported for L‐lactic acid‐producing yeasts. DDBJ/EMBL/GenBank nucleotide database with Accession Nos. AB440630 and AB440631. Copyright © 2009 John Wiley & Sons, Ltd.
Value of Both WHO and TNM Classification Systems for Patients with Pancreatic Endocrine Tumors: Results of a Single‐Center Series
Authors: Casadei Riccardo , Ricci Claudio , Pezzilli Raffaele , Campana Davide , Tomassetti Paola , Calculli Lucia , Santini Donatella , Antonacci Nicola , Minni Francesco 
Published: August 2009

AbstractObjectivesThis study was designed to evaluate the clinical relevance of the World Health Organization (WHO) and tumor node metastasis (TNM) classifications in patients affected by pancreatic endocrine tumors.MethodsData from 76 consecutive patients with pancreatic endocrine tumors who underwent surgery were analyzed.ResultsWell‐differentiated tumors were observed more frequently (57.9%) than well or poorly differentiated carcinomas (26.3% and 15.8%, respectively). The TNM stage was I in 27.6%, II in 39.5%, III in 19.7%, and IV in 13.2%. Univariate analysis of disease‐specific survival showed that patients with stages I–II had a significantly better survival rate than those with stages III–IV (hazard ratio (HR), 12.46; 95% confidence interval (CI), 1.53–101.32; P = 0.018; HR, 25.74; 95% CI, 3.07–216.07; P = 0.003, respectively). Regarding the WHO classification, poorly differentiated carcinomas had the worst prognosis (HR, 79.13; 95% CI, 9.99–626.60; P < 0.001). Multivariate Cox regression analysis of disease‐specific survival showed that the WHO classification is the only independent factors of improved survival: both poorly and well‐differentiated carcinomas had an increased risk of death compared with WDTs (HR, 100.42; 95% CI, 12.16–829.40; P < 0.001; HR, 10.73; 95% CI, 1.12–104.17; P = 0.040, respectively). TNM classification and the WHO system are highly correlated (P < 0.001).ConclusionsTNM stage and the WHO classification seems to be equally reliable, even if TNM classification tends to understage the patients classified using the WHO system.
Management of neuronopathic Gaucher disease: Revised recommendations
Authors: Vellodi A. , Tylki‐Szymanska A. , Davies E. H. , Kolodny E. , Bembi B. , Collin‐Histed T. , Mengel E. , Erikson A. , Schiffmann R. 
Published: August 2009

SummaryThe original guidelines drawn up for the management of the neuronopathic forms of Gaucher disease were felt to be in need of revision; in particular, the role of high‐dose enzyme replacement therapy (120 IU/kg of body weight every 2 weeks) in stabilizing neurological disease. The existing published evidence was analysed; it was concluded that it did not support the role of high‐dose ERT, although this might be required to treat severe visceral disease.
Systematic review of school‐based prevention and early intervention programs for depression
Authors: Calear Alison L. , Christensen Helen 
Published: August 2009

ABSTRACTA systematic review was conducted to identify and describe school‐based prevention and early intervention programs for depression and to evaluate their effectiveness in reducing depressive symptoms. Forty‐two randomised controlled trials, relating to 28 individual school‐based programs, were identified through the Cochrane Library, PsycInfo and PubMed databases. A large proportion of the programs identified were based on cognitive behavioural therapy (CBT), and delivered by a mental health professional or graduate student over 8–12 sessions. Indicated programs, which targeted students exhibiting elevated levels of depression, were found to be the most effective, with effect sizes for all programs ranging from 0.21 to 1.40. Teacher program leaders and the employment of attention control conditions were associated with fewer significant effects. Further school‐based research is required that involves the use of attention controls, long‐term follow‐ups and which focuses on the training and evaluation of sustainable program leaders, such as teachers.
A Prospective Study of Surgeon and Patient-Perceived Outcome following Transobturator Tape Insertion for Treatment of Urodynamic Stress Incontinence
Authors: Harding C.K. , Horsburgh B. , Page T. , Thorpe A.C. 
Published: August 2009

Introduction: Transobturator tape (TOT) placement for stress incontinence (USI) is recommended following failure of conservative therapy. The literature shows that in the short-term efficacy is comparable to tension-free vaginal tape (TVT) and open colposuspension (OC). The study aim was to report a large consecutive cohort undergoing TOT insertion by a single surgeon specifically for both surgeon- and patient-perceived outcome. Patients and methods: Over 4 years at our institution 98 women underwent TOT insertion by a single surgeon. All women had preoperative urodynamics, 79% had type I and 21% type IIa USI. Perioperative and outpatient follow-up data were prospectively collected. Patient-perceived outcome was assessed using a mailed validated questionnaire. Results: Results are similar to other published series. Median operative time was 19 min (15–30) and blood loss 21 mls (15–60). Self-catheterisation was necessary for 13 patients. Vaginal perforation occurred in two patients. At follow-up (mean 7.1 months) 86/93 (92%) patients were dry and 23 (26%) women reported urinary symptoms. 80 (81%) women responded to the questionnaire (mean 18 months) and 54 (68%) describing complete cure. Conclusions: This large single-surgeon series confirms the safety and efficacy of TOT and illustrates the difference between surgeon and patient-perceived outcome. Results obtained are comparable to previously published series for TOT, TVT and OC.
When, how much and what foods are eaten are related to total daily food intake
Authors: de Castro John M. 
Published: August 2009

Intake in the morning is associated with a reduction in the total intake for the day, while intake at night is associated with greater overall daily intake. These associations are macronutrient specific, with morning carbohydrate intake associated with reduced daily carbohydrate intake, morning fat intake associated with reduced daily fat intake and morning protein intake associated with reduced daily protein intake. Since different types of foods contain differing proportions of macronutrients, the present study investigated the associations of different types of foods ingested at various times of day with total daily and macronutrient intakes. The intakes of 388 male and 621 female free-living individuals reported in 7 d diet diaries were reanalysed. The intakes of twenty-four different types of foods and seven different drinks occurring during the morning (04.00–10.29 hours), afternoon (10.30–16.59 hours) and evening (17.00–02.00 hours) were identified and related to overall daily intakes. Dairy foods, ice cream, beef, other meats, potatoes, pastry, nuts, chips and snacks, condiments, alcohol and soda were significantly associated with higher total intake over the day, while fruit, soup, breakfast cereal, pasta, pizza, water, coffee/tea and diet soda were either not associated or were associated with lower overall intake. Dietary energy density appeared to mediate the associations between particular foods and beverages and overall energy intake. This suggests that eating low-density foods in the morning and avoiding high-density foods at night might aid in reducing overall intake and may be useful in dietary interventions for overweight and obesity.