bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of the transition state of Lysozyme unfolding. I. Effect of protein‐solvent interactions on the transition state
Authors: Segawa Shin‐Ichi , Sugihara Mitsuru 
Published: July 2010

AbstractThe influence of proline cis‐trans isomerization on the kinetics of lysozyme unfolding was examined carefully according to the theory of Hagerman and Baldwin [(1976) Biochemistry 15, 1462–1473]. As a result, the kinetics of lysozyme unfolding was found to follow the two‐state transition model well. The temperature dependencies of kuf and kf over a wide temperature range showed that ΔC = 0 and ΔC = −6.7 kJ K−1 mol−1 in solutions of different concentrations of GuHCl. The data observed in solutions containing other denaturants also supported the conclusion that ΔC is nearly equal to zero. The activation enthalpies of unfolding (ΔH) were observed at various concentrations of several kinds of denaturants. They were independent of species and concentrations of denaturants ΔH = 200 kJ mol−1). These facts indicate that the aspect of interaction between protein and different kinds of solvent molecules varies only slightly during the unfolding to the transition state, that is, the transition state is at compact as the native one. Therefore, it is also suggested that ΔH of 200 kJ mol−1 is primarily required for the disruption of long‐range interactions among different structural domains through a subtle conformational change. We compared the effects of several kinds of denaturants on the unfolding rate. The addition of PrOH more remarkably increases the unfolding rate than do other hydrophilic denaturants. This is probably because PrOH molecules can penetrate into the hydrophobic core of lysozyme, but hydrophilic reagents cannot because of the compactness of the transition state.
The DNA Phosphate orientation. Infrared data and energetically favorable structures
Authors: Pohle Walter , Zhurkin Victor B. , Fritzsche Hartmut 
Published: July 2010

AbstractThe conformation of nucleic acids can be probed reliably by the geometrical arrangement of the PO2‐ groups in the backbone. The phosphate arrangement is described by two angles, θOO and θOPO, which can be determined by several methods. In the present paper, the two angles obtained for the A, B, and C forms of DNA, experimentally by ir linear dichroism (LD) of oriented films as well as theoretically from energy‐minimized structures. The methodology of the phosphate angle determination from the ir dichroism spectra is presented in detail, elucidating the potentialities and limitations of this method. Advantageously, tilt and twist angles of the DNA bases, found by theoretical calculations, were used in the ir data processing. The phosphate angles were compared with the corresponding x‐ray and nmr results from the literature. Regarding the rather high flexibility of the double helix, as well as the sequence‐dependent variation of the conformational angles, a fairly good agreement between our results and the majority of all discussed data can be established. Thus ir LD provides reliable data on the orientation of phosphate groups in DNA. Reasons for discrepancies with some literature data from x‐ray fiber‐diffraction analysis, published several years ago, will be discussed.
Low‐Temperature conformation of Mg2+‐Poly(U) in D2O as revealed by IR and Raman Spectroscopy and by normal‐mode analysis treatment
Authors: Bandekar Jagdeesh , Zundel Georg 
Published: July 2010

AbstractInfrared and Raman spectra of the Mg2+ salt of poly(U) in D2O were recorded in the 1600‐1800 cm−1 region and between 1 and 20C. The ir spectra showed a melting curve similar to the uv melting curves with a temperature of transition of about 6.5°C. This spectral change is assumed to be associated with the formation of the secondary structure of Mg2+‐poly(U) in D2O at this temperature. Three double‐helical and two triple‐helical structures were used as inputs to compute the normal modes of vibration. A double‐helical structure was found to give the best agreement with the observations. Knowledge of the C=0 eigenvectors, and of the expression for transition probability from quantum mechanics, was used to explain the so far unanswered question of H. T. Miles [(1964) Proc. Natl. Acad. Sci. USA 51, 1104–1109; (1980) Biomolecular Structure, Conformation, Function and Evolution, Pergamon, Oxford, pp. 251–264] as to why there is an increase in the ir vibrational wave number of a carbonyl band when that group is H‐bonded to another polynucleotide chain in a helix. Such considerations also explain why a predicted band at about 1648 cm−1 is not to be seen in the ir spectra but is present in the Raman spectra. The model incorporating the CO transition dipole‐dipole coupling interaction is able to explain also the observed higher intensity of the higher wave‐number ir band. The experimental results demonstrate that the complete picture of vibrational dynamics of Mg2+‐poly(U) in D2O is obtained only by looking simultaneously at ir and Raman spectra and not at only one of them. Weak ir bands were found to be as useful as the strong ones in understanding structure and vibrational dynamics. On the bases of our ir and Raman spectra, of the normal‐mode analyses, and of the literature data, it is concluded that Mg2+‐poly(U) in D2O is present in a double‐helical structure at temperatures below the temperature of transition, whereby the uracil residues are paired according to arrangement (a) (see Fig. 1). This structure is rodlike and arises by refolding of one poly(U) chain. The computations show that no normal mode is associated with a single CO group vibration; all CO group vibrations are heavily mixed motions of various CO groups.
On the first‐neighbor analysis of nucleic acid CD spectra: The definitive T Matrix and considerations of various methods
Authors: Allen Fritz S. , Gray Donald M. , Ratliff Robert L. 
Published: July 2010

AbstractThe first‐neighbor approximation for the representation of double‐stranded polynucleotide CD has been widely applied. Two apparently different formalisms have appeared. We discuss and compare those formalisms and show them to be identical. We present an updated optimum basis set of polydeoxynucleotide CD spectra and the resulting first‐neighbor unit contribution matrix derived from these data.
Utility of high-resolution MR imaging to assess fibrous cap thickness, lipid-rich necrotic core and haemorrhage of carotid atheroma
Authors: Sadat U , Young V E , Graves M J , Gaunt M E , Varty K , Gillard J H 
Published: July 2010

Abstract
The following abstracts are from papers presented at the 43rd annual scientific meeting of the Vascular Society of Great Britain and Ireland, held in Bournemouth 12–14 November 2008. The President of the Society, Mr MJ Gough, was in the Chair. The BJS Prize was won by Mr TY Tang from Cambridge University Hospital, and the Founder's Prize was won by Mr PJE Holt from St George's Regional Vascular Institute, London.
Role of wound macrophages in skin flap loss or survival in an experimental diabetes model
Authors: Schürmann C , Seitz O , Sader R , Pfeilschifter J , Goren I , Frank S 
Published: July 2010

AbstractBackgroundTightly controlled wound inflammation is a central determinant of skin flap survival in healthy mice. This study investigated inflammatory response patterns in caudally pedicled skin flaps in diabetic mice during severely impaired conditions of necrotic skin flap tissue loss.MethodsSkin flap biopsies were analysed by RNase protection assay, quantitative real-time polymerase chain reaction, immunohistochemistry, enzyme-linked immunosorbent assay and immunoblotting.ResultsSkin flaps were characterized by the necrotic loss of tissue starting from distal areas of the flaps in diabetic mice. Decay of epidermal and dermal structures within skin flap tissue was paralleled by an immune cell-mediated expression of chemokines (macrophage inflammatory protein 2, macrophage chemoattractant protein 1), cyclo-oxygenase (COX) 2 and inducible nitric oxide synthase (iNOS). Distal regions of necrotic skin flap tissue were infiltrated by excess numbers of neutrophils and macrophages, and the latter were polarized towards a proinflammatory state as they expressed COX-2 and iNOS. Experimental depletion of inflammatory macrophages inhibited necrotic destruction of the distal skin flap tissue in diabetic mice despite the persistence of neutrophil infiltration and inflammation.ConclusionWound macrophages play a pivotal role in determining the survival or loss of skin flap tissue under disturbed wound healing conditions in obese diabetic mice.
Determination of serum cholestanol by semi‐micro high‐performance liquid chromatography with electrochemical detection
Authors: Hojo Kazuhiro , Hakamata Hideki , Takahashi Aya , Hosokawa Yu‐Ya , Kusu Fumiyo 
Published: July 2010

AbstractA simple and sensitive method that dose not require derivatization for determining cholestanol has been developed using HPLC with electrochemical detection (HPLC‐ECD). The current peak height was linearly related to the amount of cholestanol injected, ranging from 1 to 200 μM (r = 0.999). The detection limit (S/N = 3) of cholestanol was 0.23 μM (1.2 pmol). Total cholestanol in control human and mouse serum was determined by the present method with a recovery rate of more than 90% and an RSD (n = 5) of less than 7.3%. Further, this method was successfully applied to monitor experimental hypercholestanolemia in mice fed a high‐cholestanol diet, an animal model of cerebrotendinous xanthomatosis (CTX). In conclusion, we found this method to be both simple and useful for the determination of cholestanol in serum, helping in the diagnosis of CTX. Copyright © 2009 John Wiley & Sons, Ltd.
Relationships between structure, retention and biological activity of some Schiff base ligands and their complexes
Authors: Baošić Rada , Radojević Ana , Radulović Milanka , Miletić Srd̄an , Natić Maja , Tešić Živoslav 
Published: July 2010

AbstractThe lipophilicity of a series of Schiff base ligands and their complexes with nickel(II) and copper(II) has been determined by reversed‐phase thin‐layer chromatography using binary dioxane–water mobile phase. Chelate ligands were prepared by condensation of diamine and the corresponding β‐diketone. Copper(II) and nickel(II) complexes with chelate ligands containing ethane‐1,2‐diamine or propane‐1,2‐diamine as the amine part and pentane‐2,4‐dione and/or 1‐phenylbutane‐1,3‐dione, pentane‐2,4‐dione and/or 1,1,1‐trifluoropentane‐2,4‐dione, or 1,1,1‐trifluoropentane‐2,4‐dione and/or 1‐phenylbutane‐1,3‐dione as the β‐diketone part were synthesized. Some of investigated compounds were screened for their in vitro antifungal activity against Sacharomyces cerevisiae and antibacterial activity against Escherichia coli. Chromatographically obtained lipophilicity parameters were correlated both with calculated n‐octanol–water partition coefficient C log P and antimicrobial activities. Satisfactory correlations were obtained. Chromatographic data proved to be reliable parameters for describing the lipophilic properties of the investigated compounds. Additionally, the principal components analysis was performed on the data chromatographically obtained. This statistical method was useful for distinguishing compounds and objective comparison of their lipophilicity parameters. Copyright © 2007 John Wiley & Sons, Ltd.
Home‐made PIC 16F877 microcontroller‐based temperature control system for learning automatic control
Authors: Khairurrijal  , Abdullah Mikrajuddin , Budiman Maman 
Published: July 2010

AbstractA closed‐loop temperature control system, which is composed of a thermal plant and a controller, has been developed to support undergraduate students in learning automatic control delivered in the Special Topics in Instrumentation Physics course. The thermal plant was made from a plastic box covering a lamp and a fan, which heats and drains the air in the plastic box, respectively, as well as a temperature sensor. The controller with a proportional control action was realized by employing the PIC 16F877 microcontroller. The control signal updates pulse‐width modulators (PWMs) in which driver circuits turn on or off the lamp and the fan. A mathematical model of the closed‐loop control system was derived and a theoretical transient response was then obtained. It is found that the experimental transient responses were always much lower than the set point and the steady‐state errors were high for the proportional sensitivity (KP) lower than 10. For KP higher than 10, the transient responses tend to approach the set point to cause small steady‐state errors. These characteristics are consistent with the theoretical transient response. Further examination revealed that the closed‐loop system is a higher order system due to the action of the PWMs and the driver circuits. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 19: 10–17, 2011
Engineering education on supply‐chain management for students and for employees in industry
Authors: Ilie‐Zudor Elisabeth , Macchi Marco , Monostori László , Scotti Stefano , Kemény Zsolt 
Published: July 2010

AbstractThe paper presents a virtual institute, built in the form of an Internet‐based platform, for distance learning in the field of supply‐chain management. The Institute addresses students and teachers on university educational level, as well as employees in industry. Besides learning modules, the platform provides users with means to communicate, respectively to organise and execute project activities from remote locations. Means for on‐line content editing, and course administration are made available as well. © 2009 Wiley Periodicals, Inc. Comput Appl Eng Educ 19: 81–88, 2011
Strategic learning of simulation and functional analysis in the design and assembly of mechanisms with CAD on a professional Master's degree course
Authors: Barbero Basilio Ramos , García García Rafael 
Published: July 2010

AbstractCurrently, programs that teach 3D CAD as part of an industrial engineering qualification in Spanish universities are structured around four pillars: solid design, surface design, model assemblage, and the drafting of plans. In this article, we add two new pillars: 3D CAD simulation and kinematic analysis of both mechanisms and assemblages, as part of the professional master's degree course entitled “Master's Degree in automotive components.” We should stress the novel content selected for this advanced CAD subject matter, in accordance with the needs of a group of firms and our prior experience of component design. Moreover, we recount our experiences, over two academic years, of student learning processes when using the EHEA‐based learning method that we refer to as “strategic knowledge.” The various innovative improvements throughout this experimental teaching approach were subjected to qualitative analysis. © 2009 Wiley Periodicals, Inc. Comput Appl Eng Educ 19: 146–160, 2011
Practice tool based on open source SCADA for experimentation in nonlinear control using the inverted pendulum
Authors: García‐Nieto S. , Martínez M. , Salcedo J. , Laurí D. 
Published: July 2010

AbstractThis paper presents the potential of open source software for designing educational tools in the automatization field. In particular, this paper presents a complete tool that students can use for studying and testing nonlinear control algorithms. The system has three different parts that students can evaluate and modify. Firstly, a virtual model represents the physical model and, in this case, an inverted pendulum is used. Secondly, the controller is implemented by a real‐time distributed control system. Finally, the system can be managed with a JAVA application. Therefore, students have all the necessary elements to practice using nonlinear and complex systems. The main tools applied in the design are open source software and the developed platform is Generalized Public License(GPL). © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 20: 137–148, 2012
Occupational health hazards in the interventional laboratory: Time for a safer environment
Authors: Klein Lloyd W. , Miller Donald L. , Balter Stephen , Laskey Warren , Haines David , Norbash Alexander , Mauro Matthew A. , Goldstein James A. ,   on behalf of the members of the Joint Inter‐Society Task Force on Occupational Hazards in the Interventional Laboratory
Published: July 2010

AbstractThis document is a consensus statement by the major American societies of physicians who work in the interventional laboratory environment. It reviews available data on the prevalence of occupational health risks and summarizes ongoing epidemiologic studies designed to further elucidate these risks. Its purpose is to affirm that the interventional laboratory poses workplace hazards that must be acknowledged, better understood, and mitigated to the greatest extent possible. Vigorous efforts are advocated to reduce these hazards. Interventional physicians and their professional societies, working together with industry, should strive toward minimizing operator radiation exposure, eliminating the need for personal protective apparel, and ending the orthopedic and ergonomic consequences of the interventional laboratory work environment. © SIR, 2009.
Results after balloon angioplasty or stenting of atherosclerotic subclavian artery obstruction
Authors: Sixt Sebastian , Rastan Aljoscha , Schwarzwälder Uwe , Bürgelin Karlheinz , Noory Elias , Schwarz Thomas , Beschorner Ulrich , Frank Ulrich , Müller Christian , Hauk Michael , Leppanen Olli , Hauswald Kirsten , Brantner Regina , Nazary Taher , Neumann Franz‐Josef , Zeller Thomas 
Published: July 2010

AbstractIntroduction:Endovascular therapy is an accepted treatment strategy in occlusive disease of the subclavian artery. The purpose of this study was to evaluate the technical and clinical long‐term outcome following either balloon angioplasty (plain balloon angioplasty (PTA)) or stenting.Patients and Methods:The authors retrospectively analyzed 108 interventions of atherosclerotic lesions of subclavian arteries or the brachiocephalic trunk (107 patients; mean age of 66 ± 9 years; 90% symptomatic) representing 92% of the patients treated with subclavian artery obstructive disease during a 10‐year period. Primary endpoint of the study was the 1‐year primary patency rate. Follow‐up was based on oscillometry, Doppler blood pressure measurements, and duplex ultrasound.Results:The primary success rate was 97% (100% for stenoses (78/78) and 87% for total occlusions (26/30)). Treatment modalities included PTA alone (13%; n = 14) or stenting (87%; n = 90) with balloon‐expandable (n = 61), self‐expanding (n = 17), or both types of devices (n = 12). The 1‐year primary patency rate of the 97 patients eligible for follow‐up was 88%, for the subgroups 79% (PTA) and 89% (stenting; P = 0.2). The blood pressure difference between both limbs at baseline was 45 ± 26 mm Hg and dropped to 10 ± 14 mm Hg (P < 0.001) after the intervention and 15 ± 19 mm Hg at 1 year (P < 0.01).Conclusion:Endovascular therapy of atherosclerotic subclavian artery obstructions result in excellent acute success rates even in total occlusions. Results evince good durability of endovascular therapy for atherosclerotic occlusive disease of subclavian arteries and with a trend towards better outcome with stenting compared to PTA. © 2009 Wiley‐Liss, Inc.
Mesoporous Indium Tin Oxide as a Novel Platform for Bioelectronics
Authors: Frasca Stefano , von Graberg Till , Feng Jiu‐Ju , Thomas Arne , Smarsly Bernd M. , Weidinger Inez M. , Scheller Frieder W. , Hildebrandt Peter , Wollenberger Ulla 
Published: July 2010

AbstractStable immobilization and reversible electrochemistry of cytochrome c in a transparent indium tin oxide film with a well‐defined mesoporosity (mpITO) is demonstrated. The transparency and good conductivity, in combination with the large surface area of mpITO, allow the incorporation of a high amount of electroactive biomolecules and their electrochemical and spectroscopic investigation. UV/Vis and resonance Raman spectroscopy, in combination with direct protein voltammetry, are employed for the characterization of cytochrome c immobilized in the mpITO and reveal no perturbation of the structural integrity of the redox protein. The potential of this modified material as a biosensor for detection of superoxide anions is also demonstrated.
ChemInform Abstract: Use of Cyclic α,β‐Unsaturated Trifluoromethyl Sulfones in Diels—Alder Reactions and Michael Additions.
Authors: Billard Thierry , Langlois Bernard R. , Essers Michael , Haufe Guenter 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Structural Characterization of the Li Argyrodites Li7PS6 and Li7PSe6 and Their Solid Solutions: Quantification of Site Preferences by MAS‐NMR Spectroscopy.
Authors: Kong Shiao Tong , Guen Oezguel , Koch Barbara , Deiseroth Hans Joerg , Eckert Hellmut , Reiner Christof 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Low Thermal Expansion MBa(Co,M′)4O7 Cathode Materials Based on Tetrahedral‐Site Cobalt Ions for Solid Oxide Fuel Cells.
Authors: Kim Jung‐Hyun , Manthiram Arumugam 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Room‐Temperature Synthesis Leading to Nanocrystalline Ag2V4O11.
Authors: Sauvage Frederic , Bodenez Vincent , Tarascon Jean‐Marie , Poeppelmeier Kenneth R. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of N,N‐Bis(nonaflyl) Squaric Acid Diamide and Its Application to Organic Reactions.
Authors: Cheon Cheol Hong , Yamamoto Hisashi 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Simple and Highly Efficient Catalyst‐ and Waste‐Free Ring Opening of Epoxides with Na2S in Water.
Authors: Azizi Najmedin , Akbari Elham , Ebrahimi Frough , Saidi Mohammad R. 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Mild, Efficient, and Regioselective Monobromination of Arylamines and Phenols Using [BBIm]Br3 as a New Reagent.
Authors: Borikar Sanjay P. , Daniel Thomas , Paul Vincent 
Published: July 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.