bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protosappanin B enhances the chemosensitivity of 5-fluorouracil in colon adenocarcinoma by regulating the LINC00612/microRNA-590-3p/Golgi phosphoprotein 3 axis
Authors: Hong Zhongshi , Li Yachen , Chen Mingliang , Chen Xiaojing , Deng Xian , Wu Yuze , Wang Chunxiao , Qiu Chengzhi 
Published: May 2024

Abstract
Background
5-fluorouracil (5-FU) is conventionally used in chemotherapy for colon adenocarcinomas. Acquired resistance of 5-FU remains a clinical challenge in colon cancer, and efforts to develop targeted agents to reduce resistance have not yielded success. Protosappanin B (PSB), the main component of Lignum Sappan extract, is known to exhibit anti-tumor effects. However, whether and how PSB could improve 5-FU resistance in colon cancer have not yet been established. In this study, we aimed to explore the effects and underlying mechanisms of PSB in 5-FU-induced chemoresistance in colon adenocarcinoma.

Methods
Forty-seven paired colon cancer tissue samples from patients who received 5-FU chemotherapy were collected as clinical samples. Two 5-FU resistant colon cancer cell lines were established for in vitro experiments. Reverse transcription-quantitative PCR (RT-qPCR) was performed to determine the mRNA and microRNA (miRNA) expression levels in colon adenocarcinoma tissues and cell lines. Cell Counting Kit-8 (CCK-8) and flow cytometry assays were performed to evaluate cell proliferation and apoptosis, respectively.

Results
LINC00612 was highly expressed in colon adenocarcinoma samples and 5-FU resistant colon cancer cells. LINC00612 knockdown enhances 5-FU chemosensitivity in 5-FU resistant cells. Notably, PSB treatment attenuated LINC00612 expression in 5-FU resistant colon adenocarcinoma cells. Moreover, PSB treatment reversed the increase in LINC00612-induced 5-FU resistance. Mechanistically, LINC00612 specifically bound to miR-590-3p, which promoted 5-FU resistance in colon adenocarcinoma cells and attenuated the inhibitory effect of LINC00612 on GOLPH3 expression.

Conclusion
PSB attenuates 5-FU chemoresistance in colon adenocarcinoma by regulating the LINC00612/miRNA-590-3p/GOLPH3 axis.

Graphical Abstract
Challenges in assessing the immunization status of adults in Germany—lessons from a population-based VACCELERATE survey on polio vaccination
Authors: Nacov Julia A. , Stemler Jannik , Salmanton-García Jon , Cremer Louise M. , Zeitlinger Markus , Mallon Patrick W. G. , Pana Zoi Dorothea , Schmitt Heinz-Josef , Cornely Oliver A. ,   On behalf of the VACCELERATE Consortium
Published: May 2024

Abstract
Purpose
Considering the re-emergence of poliomyelitis (PM) in non-endemic regions, it becomes apparent that vaccine preventable diseases can rapidly develop epi- or even pandemic potential. Evaluation of the current vaccination status is required to inform patients, health care providers and policy makers about vaccination gaps.

Methods
Between October 28 2022 and November 23 2022, 5,989 adults from the VACCELEREATE Volunteer Registry completed an electronic case report form on their previous PM vaccine doses including number, types/-valencies and the time of administration based on their vaccination records. A uni-/multivariable regression analysis was performed to assess associations in participant characteristics and immunization status.

Results
Among German volunteers (n = 5,449), complete PM immunization schedule was found in 1,981 (36%) participants. Uncertain immunization, due to unknown previous PM vaccination (n = 313, 6%), number of doses (n = 497, 9%), types/-valencies (n = 1,233, 23%) or incoherent immunization schedule (n = 149, 3%) was found in 40% (n = 2,192). Out of 1,276 (23%) participants who reported an incomplete immunization schedule, 62 (1%) never received any PM vaccine. A total of 5,074 (93%) volunteers reported having been vaccinated at least once and 2,087 (38%) indicated that they received vaccination within the last ten years. Female sex, younger age, as well as availability of first vaccination record were characteristics significantly associated with complete immunization (p < 0.001).

Conclusion
Full PM immunization schedule was low and status frequently classified as uncertain due to lack of details on administered doses. There is an obviousneed for improved recording to enable long-term access to detailed vaccination history in the absence of a centralized immunization register.
Smoking habits and osteoporosis in community-dwelling men subjected to dual-X-ray absorptiometry: a cross-sectional study
Authors: Vergatti A. , Abate V. , D’Elia L. , De Filippo G. , Piccinocchi G. , Gennari L. , Merlotti D. , Galletti F. , Strazzullo P. , Rendina D. 
Published: May 2024

Abstract
Background
Active and Environmental Tobacco Smoke (ETS) are a global cause of death. Osteoporosis (Op) is the most common metabolic bone disorder worldwide, impacting on mortality and disability, with high health and welfare costs. Active smoking is a known risk factor for Op, but there is few information regarding Op and ETS in men.

Purpose
The study aim is to evaluate the association between smoking habits and Op in community-dwelling men that have been subjected to Dual-X-ray Absorptiometry and completed a questionnaire about their own and cohabiter’s smoking habits.

Methods
We performed a cross-sectional study based on administrative data. This study is part of the SIMON protocol. The binary logistic regression analysis was used to estimate the role of ETS on the risk of Op, adjusting for age, body mass index (BMI), type 2 diabetes mellitus (T2DM) and eGFR.

Results
Four hundred sixteen men were selected and, based on questionnaire replies, 167 were classified as current smokers (CS), 93 as passive smokers (PS) and 156 as never smokers (NS). NS showed a lower prevalence of past fragility fracture, radiological features of osteoporosis and hypovitaminosis D compared to PS and CS (p < 0.05). NS showed a lower prevalence of Op compared to PS and CS, also after correction for age, BMI, T2DM and eGFR (p < 0.05).

Conclusion
The study results demonstrate that PS and CS have a higher risk of Op, fragility fractures and vitamin D deficiency compared to NS.
A Strategic NH3-Dosing Approach for the Minimization of N2O Production During NH3-SCR Reactions over Cu-SSZ-13 Catalysts
Authors: Nasello Nicole Daniela , Usberti Nicola , Nova Isabella , Tronconi Enrico , Hofmann Frank , Dieterich Simone , Crocoll Marcus , Weibel Michel 
Published: May 2024

AbstractThe undesired production of N2O during NH3-SCR reactions is investigated over a reference commercial Cu-CHA catalyst. Steady-state experiments performed in the 150–500 °C temperature range exhibit a bimodal trend in the N2O formation profile, confirming the existence of two different reaction mechanisms occurring at low and high temperatures. Focusing on a low-to-medium T-range, N2O production, usually ascribed to NH4NO3 formation and decomposition, increases with the NO2/NOx ratio. However, an excess of NO2 leads to a decrease in the N2O release due to ammonium nitrate deposition and catalyst clogging phenomena. Steady-state and dynamics experiments show the promoting effect of both NH3 feed concentration and NH3 storage on N2O production at T > 200 °C. Surprisingly, N2O decreases with increasing NH3/NOx ratio at lower temperature. A novel approach based on the strategic injection of NH3 is also applied to mitigate the N2O formation while maintaining high deNOx activity. Remarkably, complete NOx conversion and ~ 11% N2O saving are achieved (with inlet NO2/NOx = 0–0.5) at temperatures exceeding 200 °C; in addition, a peculiar behavior is observed in the N2O profile, which increases and decreases when adding and removing NH3 from the feed, respectively. Notably, the opposite trend is observed in the N2O profile at 200 °C. When under Standard SCR conditions, this so far unreported observation challenges the NH4NO3 formation route for N2O and suggests the existence of different controlling phenomena at different temperature regimes: i) the Cu/redox chemistry at T ≤ 200 °C and ii) the NH3 storage at higher temperature, ideally up to 300 °C.
Logistik des Schönen
Authors: Stöckmann Ingo 
Published: May 2024

Zusammenfassung-Der Aufsatz rekonstruiert zunächst Bernard Bolzanos Ästhetik als paradigmatisches Exempel für die in Deutschland marginalisierte logisch-semantische Ästhetik-Tradition in der Leibniz-Nachfolge und schließt mit Perspektiven für eine künftige Rekonstruktion der nachhegelschen Ästhetik im Zeichen des Bedeutungsproblems, wie es zu Beginn des 20. Jahrhunderts im Kontext von Semantik, sprachanalytischer Philosophie und Linguistik virulent wird. Damit rückt das vermeintlich idealistisch geprägte 19. Jahrhundert in eine Kontinuität mit Theorieentwicklungen, die im Gefolge des linguistic turn die unhintergehbare Sprachlichkeit und zeichenhafte Strukturiertheit aller Bedeutung betonen.
Smart home energy management systems in India: a socio-economic commitment towards a green future
Authors: George Thomas , Selvakumar A. Immanuel 
Published: May 2024

AbstractA smart home energy management system plays an important role in improving the efficiency of an energy distribution system and also helps to reduce the carbon footprint of the power utility company. For a developing country like India, one of the main challenges faced while integrating an energy management system and renewable energy technology is the migration cost faced by the user from the existing system. The existing energy policy of the nation or the community should be reformed in such a way that the user who is willing to adapt to an energy management system should be properly rewarded. Smart appliances and IoT-enabled devices reduce wiring complexity in any conventional home and the smart metering facility aids in the bidirectional communication between consumers and utility companies. But how does it take care of user privacy? What are the reasons behind the user’s negligence on-demand response schemes in India? Through a case study, it was observed that the power consumption of domestic consumers in India increased over the years. It was also observed through an energy survey of 200 low-tension domestic consumers that a simple reengineering of lighting loads can save up to 4.68 Megawatt-hour of energy in a year. The paper also identified the negative impact of the inclining block rate billing scheme by comparing the bimonthly energy consumption pattern of consumers and also proposed a new billing scheme. The paper also reviews the types of optimization methods available for load scheduling. This paper is an attempt to enlighten readers on the importance of adopting a sustainable home energy management system, as a socio-economic commitment towards a green future.
A novel membrane-less microbial fuel cell reactor using wood as container and separator to prevent air–cathode deterioration and biofouling
Authors: Tahir Chenar A. , Agarwal Charu , Pásztory Zoltán , Csóka Levente 
Published: May 2024

AbstractIn this study, we introduce a novel application of wood as the construction material for Microbial Fuel Cell (MFC) containers, developing what we term the Wooden Membrane-less Microbial Fuel Cell (WML-MFC). This innovative approach leverages the natural properties of wood to enhance the sustainability and cost-effectiveness of MFCs. Employing three different types of wood—pine, oak, and black locust—the WML-MFC design incorporates a carbon felt anode inside the wooden container and a carbon cloth cathode wrapped externally. This configuration not only protects the cathode from biofouling but also utilizes the inherent moisture management capabilities of wood to maintain operational stability. The performance of these wooden MFCs was assessed in terms of electricity generation and water treatment efficacy. Pine and oak containers achieved maximum power densities (MPD) of 35 mW/m^2 and 4 mW/m^2, respectively, with corresponding maximum open-circuit voltages of 551 mV and 269 mV. Black locust showed the least effective bioelectricity generation. COD removal efficiency was observed between 18 and 48% for pine and 3% to 39% for oak over hydraulic retention times of 24–48 h. Notable water loss due to moisture diffusion was recorded at 20%/day in pine and 6%/day in oak. Durability assessments through DMA and SEM analyses confirmed the suitability of wood as a container material, emphasizing the dual environmental and economic benefits of this WML-MFC design.
Understanding the evolution of China’s approach to digital trade: interests, ideas, and institutions
Authors: Zhang Danni 
Published: May 2024

AbstractAmid economic globalisation and the rapid development of advanced technologies in recent years, the world economy has shown an increasing trend of digitalisation, whilst digital trade has become the primary pattern of international trade. Based on differences rooted in socio-political systems, as well as the current precarious geopolitical realities, governments adopt different ideas in accordance with their respective interests in the institutionalisation of digital trade. As the world’s second-largest economy and a major digital power, China’s behaviours and ideological stances attract scholarly attention and states’ concern. Whilst Western countries and scholars have criticised China’s ideas of “government intervention in data regulation” and “cyber sovereignty” applied in its existing approach to digital trade, warning that China’s approach poses a threat to Western powers’ adherence to liberalism in the international digital trade system and the idea of “free data flow” in their approaches to digital trade, there has been insufficient research into how China’s approach to digital trade has evolved over time and which ideas and interests have influenced it. To bridge this gap in the literature, this paper divides the evolution of China’s approach to digital trade over the past three decades (1993–2023) into three phases using a longitudinal approach. This research conducts qualitative content analysis using official documents, white papers, and declarations from official Chinese websites, then adopts the 3I analytical framework to analyse which ideas and interests have influenced China’s approach in each phase and how its approach to digital trade has evolved over time.
Accuracy of elastography versus biopsy in assessing severity of liver fibrosis in young Fontan patients
Authors: Bulut Ozlem P. , Bailey Smita S. , Bhat Deepti P. 
Published: May 2024

AbstractObjectives:We performed a single-centre retrospective study comparing the accuracy of non-invasive elastography with liver biopsy in accurate assessment of Fontan-associated liver disease.Methods:Fontan patients who underwent combined assessment with a percutaneous liver biopsy and non-invasive elastography between January 2015 and December 2023 at our Children’s hospital were included. Liver biopsies were classified using the Congestive Hepatic Fibrosis Score as early Fontan-associated liver disease (scores 1, 2) and advanced Fontan-associated liver disease (score 3/bridging fibrosis and score 4/cirrhosis). Elastography values were categorised as advanced Fontan-associated liver disease for liver elasticity >2.1 m/s by ultrasound and liver stiffness >5 KPa on magnetic resonance elastography.Results:We included 130 patients (116 children, 89%, mean age at biopsy: 14.6 years ± 3.6) who underwent liver biopsy at a mean duration of 11.1 years (±0.3) following Fontan surgery. Advanced Fontan-associated liver disease was noted in 41 (31.5%) patients with 13 (10%) showing frank cirrhosis. Pre-biopsy ultrasound showed advanced liver fibrosis in 18/125 (14%), with low sensitivity (23%), high specificity (90%), and low accuracy (68%, k = 0.1) in diagnosing advanced Fontan-associated liver disease. Similarly, pre-biopsy magnetic resonance elastography showed advanced fibrosis in 23/86 (27%) of patients, with low sensitivity (30%), fair specificity (75%), and low accuracy (63%, k = 0.1). Interestingly, advanced Fontan-associated liver disease was missed by ultrasound in 29% and by magnetic resonance elastography in 25% of patients. Advanced Fontan-associated liver disease was associated with lower platelet count (p = 0.02) and higher Gamma-glutamyl Transferase levels (p = 0.02).Conclusion:Advanced hepatic fibrosis is common among paediatric Fontan patients. Non-invasive elastography may overestimate and underestimate the degree of liver fibrosis, and therefore, liver biopsy may be required for confirming disease severity.
A Superposed Epoch Analysis of Auroral Oval Coverage During Substorms Using Deep Learning‐Based Segmentation Models
Authors: Jiang Jia‐Nan , Zou Zi‐Ming , Lu Yang , Zhong Jia , Wang Yong , Ma Yu‐Zhang , Zhao Bian‐Long 
Published: May 2024

AbstractAs an effect of solar‐terrestrial activity, the aurora has always been a focus of substorm research. The expansion of the aurora is one of the important characteristics during substorms, which could be directly reflected by the change of auroral oval coverage. However, the related studies were limited because of the absence of a reliable automated method to obtain auroral ovals from a large number of images. In this paper, we propose a new strategy to achieve this. Based on the Segment Anything Model, we design a process for annotating the auroral oval region that requires little manual work. A new aurora segmentation model, HrSeg, is then developed to obtain auroral ovals more efficiently and accurately. Through 5‐fold cross‐validation, it is determined that the average intersection over union, Dice coefficient, and pixel accuracy are all greater than 0.97. Furthermore, images of 590 substorms observed by the Polar satellite Ultraviolet Imager are segmented. We present superposed epoch analyses of the auroral oval coverage calculated from the segmentation results. Generally, the coverage decreases slightly before onset, then rapidly increases for tens of minutes after onset, and finally decreases gradually. Moreover, the auroras in different magnetic local time (MLT) sectors exhibit different evolutions in coverage. It is also revealed that the evolution pattern of auroral coverage depends on interplanetary magnetic field orientations and seasonal conditions. The results quantify the variation of auroral morphology in terms of coverage, which complete the evolution pattern of aurora during substorms and provide a more comprehensive understanding of substorms.
Parental conflict driven regulation of endosperm cellularization by a family of Auxin Response Factors
Authors: Butel N. , Qiu Y. , Xu W. , Santos-González J. , Köhler C. 
Published: May 2024

AbstractThe endosperm is a reproductive tissue supporting embryo development. In most flowering plants, the initial divisions of endosperm nuclei are not succeeded by cellularization; this process occurs only after a specific number of mitotic cycles have taken place. The timing of cellularization significantly influences seed viability and size. Previous research implicated auxin as a key factor in initiating nuclear divisions and determining the timing of cellularization. Here we uncover the involvement of a family of clustered auxin response factors (cARFs) as dosage-sensitive regulators of endosperm cellularization. cARFs, maternally expressed and paternally silenced, are shown to induce cellularization, thereby restricting seed growth. Our findings align with the predictions of the parental conflict theory, suggesting that cARFs represent major molecular targets in this conflict. We further demonstrate a recurring amplification of cARFs in the Brassicaceae, suggesting an evolutionary response to parental conflict by reinforcing maternal control over endosperm cellularization. Our study highlights that antagonistic parental control on endosperm cellularization converges on auxin biosynthesis and signalling.
Overexpression of COL11A1 confers tamoxifen resistance in breast cancer
Authors: Fu Chengxiao , Duan Shan , Zhou Xiaoming , Meng Yingcai , Chen Xisha 
Published: May 2024

AbstractBreast cancer is the most commonly diagnosed malignancy and benefits from endocrine agents such as tamoxifen. However, the development of drug resistance in cancerous cells often leads to recurrence, thus limiting the therapeutic benefit. Identification of potential biomarkers that can predict response to tamoxifen and recognize patients who will clinically benefit from this therapy is urgently needed. In this study, we report that high collagen type XI alpha 1 (COL11A1) expression was associated with poor therapeutic response and prognosis in breast cancer patients treated with tamoxifen. To confirm the role of COL11A1 in the development of tamoxifen resistance, we established MCF-7/COL11A1 and T47D/COL11A1 cell lines, which stably expressed COL11A1. Compared with parental MCF-7 and T47D, MCF-7/COL11A1 and T47D/COL11A1 cells were more resistant to 4-OHT-induced growth inhibition. Moreover, the level of COL11A1 expression was upregulated in tamoxifen-resistant MCF-7/TamR and T47D/TamR cell lines, and depletion of COL11A1 markedly sensitized the cells to 4-OHT in vitro and in vivo. Interestingly, the level of estrogen receptor α (ERα) expression was elevated, probably due to the increased COL11A1 in TamR cells. In addition, knockdown of COL11A1 decreased the expression of ERα and its downstream target genes. Overall, our findings suggest that overexpressed COL11A1 contributes to tamoxifen resistance, and targeting COL11A1 holds great promise for reversing endocrine resistance.
Miniaturized all-in-one microneedle device for point of care light therapy
Authors: Zhao Huiting , Wang Xu , Xiong Jiyuan , Liang Guomin , Wu Xin , Xi Jiyu , Zhang Yu , Li Zixi , Hu Xiaoming , Wei Zewen 
Published: May 2024

AbstractLight therapies have been applied to millions of patients for treating many kinds of diseases, especially superficial ones. Currently, mainstream light therapies utilize the combined effects of photosensitizers and light to either remove disordered tissue or promote the growth of healthy tissue. Adverse effects of light therapy, including metabolic burden caused by circulatory photosensitizer and skin damage induced by high irradiance light, are yet to be addressed. This study provides a Miniaturized all-in-one Light therapy Device (MiLD). All components required for light therapy, including dual-function microneedles, LED array, control circuit, and battery are integrated together to form a miniaturized portable device with 2 cm in length, 1.7 cm in width, 1.2 cm in height, and 3.6 g in weight. The all-in-one design and patch-to-cure operation of MiLD enables the successful demonstration of point-of-care light therapy. Satisfactory therapeutic effects have been verified in mice on both types of light therapy. Meanwhile, transdermally co-delivering both photosensitizer and light in situ fully avoids photosensitizer accumulation in blood and remarkably reduces the irradiance of light, therefore significantly alleviating metabolic burden and light-induced skin damage. Overall, the MiLD lays the technical foundation of point-of-care light therapy with its miniaturized all-in-one design, simple patch-to-cure operation, satisfactory therapeutic effects, and minimum adverse effects.