bluuebunny/crossref_metadata_2025_split huggingface dataset:
Beban Kerja dan Asupan Gizi Makro dengan Kelelahan Kerja Ahli Gizi Rumah Sakit
Authors: Wardani Wening Sulistyo , Rakhma Luluk Ria , Hidayati Listyani , Zulaekah Siti 
Published: May 2024

Beban kerja yang tidak sesuai dengan kemampuan pekerja menjadi penyebab timbulnya kelelahan kerja. Kelelahan kerja dapat dipengaruhi oleh beberapa faktor: beban kerja yang berat dan asupan yang kurang memadai. Penelitian ini bertujuan untuk mengetahui hubungan antara beban kerja dan asupan gizi makro dengan kelelahan ahli gizi rumah sakit di Wilayah Sukoharjo. Desain penelitian ini cross sectional dengan teknik stratified random sampling dari 52 populasi sehingga diperoleh sampel yang sesuai dengan kriteria inklusi sejumlah 34 responden ahli gizi. Data beban kerja diperoleh melalui observasi pencatatan aktivitas. Asupan gizi makro diperoleh melalui recall 24 jam. Data kelelahan kerja diperoleh dengan pengisian kuesioner Industrial Fatigue Research Committee. Analisis data menggunakan uji pearson product moment. Mayoritas ahli gizi mengalami beban kerja dengan kategori sedang 19 responden (55,8%) dan 23 responden (67, 6%) memiliki skor kelelahan kerja tinggi. Tidak ditemukan hubungan antara beban kerja dan asupan gizi makro dengan kelelahan kerja ahli gizi rumah sakit di Wilayah Sukoharjo, hasil uji statistik untuk beban kerja dengan kelelahan didapatkan nilai p = 0,353. Hasil uji statistik asupan gizi makro dengan kelelahan didapatkan nilai p 0,090 (energi ); p 0, 084 (protein); p 0,831 ( lemak); p 0,322 (karbohidrat).
Motivasi Berhubungan Dengan Pemanfaatan Layanan Kesehatan Penderita Hipertensi
Authors: Lende Narwi Arvince , Cahyaningrum Ika , Rosdiana Yanti 
Published: May 2024

Prevalensi hipertensi pada masyarakat secara umum terus meningkat. Ada beberapa cara untuk mengelola hipertensi, seperti memanfaatkan sumber daya kesehatan dan termotivasi untuk melakukan pengobatan. Tujuan penelitian ini adalah untuk mengetahui hubungan motivasi dengan penggunaan layanan perawatan pasien hipertensi di Kedungkandang Kota Malang. Desain penelitian menggunakan pendekatan cross-sectional. Populasi penelitian berjumlah 121 orang, dengan sampel sebanyak 93 orang penderita hipertensi. Pengambilan sampel dilakukan dengan menggunakan teknik konsekutif sampling. Variabel bebas adalah motivasi, sedangkan variabel terikat adalah pemanfaatan pelayanan kesehatan. Instrumen yang digunakan adalah lembar kuesioner pemanfaatan layanan kesehatan dan lembar kuesioner motivasi. Analisis data menggunakan uji korelasi gamma. Berdasarkan hasil penelitian dapat disimpulkan bahwa sebagian besar responden penderita darah tinggi di wilayah Kedungkandang Kota Malang memiliki tingkat motivasi sedang (64,5%); sebagian besar memanfaatkan layanan kesehatan sebesar 81,7%; dan terdapat hubungan yang kuat antara motivasi dengan pemanfaatan layanan penunjang hipertensi di Kelurahan Kedungkandang Kota Malang (pvalue =0,00 dengan r = 0,796). Saran untuk peneliti selanjutnya, melakukan penelitian mengenai faktor-faktor yang dapat mempengaruhi motivasi pada penderita hipertensi untuk memaksimalkan pemanfaatan layanan kesehatan.
Vitrification of human blastocysts for couples undergoing assisted reproduction: an updated review
Authors: Sciorio Romualdo , Tramontano Luca , Campos Gerard , Greco Pier Francesco , Mondrone Giuseppe , Surbone Anna , Greco Ermanno , Talevi Riccardo , Pluchino Nicola , Fleming Steven 
Published: May 2024

Over the past 40 years there has been a worldwide critical change in the field of assisted reproduction technology (ART), leading to the increased application of single blastocyst transfer, which is extremely important to avoid the risks of multiple pregnancy and associated complications for both mother and babies. Indeed, advancements in ART over the last few decades have been obtained thanks to several improvements, including ovarian stimulation, embryo culture conditions and, of course, progress in cryopreservation methods, especially with the application of vitrification. The ability to cryopreserve human embryos has improved significantly with vitrification compared to the initially adopted slow-freezing procedures. Since the introduction of vitrification, it has become the gold standard method to effectively cryopreserve human blastocysts. However, some new protocols are now being explored, such as the short warming procedure and even shorter exposure to the equilibration solution before vitrification, which seem to provide optimal results. Therefore, the main aim of the current narrative review, will be to illustrate the benefit of vitrification as an effective method to cryopreserve the human blastocyst and to illustrate new protocols and variations which in future may increase the performance of vitrification protocols.
Comparative analysis of oropharyngeal microbiota in healthcare workers post-COVID-19
Authors: Wei Yue , Yu Wenyi , Zhang Zhixia , Liu Siqin , Xue Jianbo , Wu Chunyan , Gao Zhancheng , Guo Shuming 
Published: May 2024

BackgroundTo date, more than 770 million individuals have become coronavirus disease 2019 (COVID-19) convalescents worldwide. Emerging evidence highlights the influence of COVID-19 on the oral microbiome during both acute and convalescent disease phases. Front-line healthcare workers are at an elevated risk of exposure to viral infections, and the effects of COVID-19 on their oral microbiome remain relatively unexplored.MethodsOropharyngeal swab specimens, collected one month after a negative COVID-19 test from a cohort comprising 55 healthcare workers, underwent 16S rRNA sequencing. We conducted a comparative analysis between this post-COVID-19 cohort and the pre-infection dataset from the same participants. Community composition analysis, indicator species analysis, alpha diversity assessment, beta diversity exploration, and functional prediction were evaluated.ResultsThe Shannon and Simpson indexes of the oral microbial community declined significantly in the post-COVID-19 group when compared with the pre-infection cohort. Moreover, there was clear intergroup clustering between the two groups. In the post-COVID-19 group, the phylum Firmicutes showed a significant increase. Further, there were clear differences in relative abundance of several bacterial genera in contrast with the pre-infection group, including Streptococcus, Gemella, Granulicatella, Capnocytophaga, Leptotrichia, Fusobacterium, and Prevotella. We identified Gemella enrichment in the post-COVID-19 group, potentially serving as a recovery period performance indicator. Functional prediction revealed lipopolysaccharide biosynthesis downregulation in the post-COVID-19 group, an outcome with host inflammatory response modulation and innate defence mechanism implications.ConclusionDuring the recovery phase of COVID-19, the oral microbiome diversity of front-line healthcare workers failed to fully return to its pre-infection state. Despite the negative COVID-19 test result one month later, notable disparities persisted in the composition and functional attributes of the oral microbiota.
Systematic review of literature to evaluate global distribution of species of the Sporothrix genus stored in culture collections
Authors: Morgado Debora Salgado , Castro Rodolfo , Ribeiro-Alves Marcelo , Corrêa-Moreira Danielly , Silva Júlio Castro Alves de Lima e , Menezes Rodrigo Caldas , Oliveira Manoel Marques Evangelista 
Published: May 2024

IntroductionSporotrichosis is a subcutaneous mycosis caused by fungi of the genus Sporothrix sp. Phenotypic and genotypic differences have been associated with their geographic distribution, virulence, or clinical manifestation of sporotrichosis. In the past decade, the interest in identifying species of the Sporothrix sp. has been increasing, due to its epidemiological importance and, in consequence, is important to know how to preserve them for future studies, in culture collection.AimsThe purposes of this study were to analyze the global distribution of environmental isolates and/or causal agents of sporotrichosis identified by polyphasic taxonomy, with mandatory use of molecular identification, and to evaluate the percentages and distribution of isolates stored in culture collections.MethodsA systematic review of articles on animal and human sporotrichosis and/or environmental isolation of the fungus, from 2007 to 2023, was done. Results: Our results demonstrated that, S. globosa, S. schenckii, and S. brasiliensis were the most identified species. With respect to the deposit and maintenance of species, we observed that only 17% of the strains of Sporothrix sp. isolated in the world are preserved in a culture collection.ConclusionsThis systematic review confirmed a difficulty in obtaining the frequency of Sporothrix species stored in culture collection and insufficient data on the molecular identification mainly of animal sporotrichosis and isolation of Sporothrix sp. in environmental samples.
Long-Term pemafibrate treatment exhibits limited impact on body fat mass in patients with hypertriglyceridemia accompanying NAFLD
Authors: Iwadare Takanobu , Kimura Takefumi , Kunimoto Hideo , Okumura Taiki , Wakabayashi Shun-Ichi , Kobayashi Hiroyuki , Yamashita Yuki , Sugiura Ayumi , Tanaka Naoki , Umemura Takeji 
Published: May 2024

AimShort-term use of pemafibrate (PEM), a selective modulator of peroxisome proliferator-activated receptor alpha, has been reported to improve abnormal liver function in patients with nonalcoholic fatty liver disease with hypertriglyceridemia (HTG-NAFLD). This study aimed to clarify the effects and predictive factors of long-term 72-week PEM administration on body composition, and laboratory tests in HTG-NAFLD patients.MethodsFifty-three HTG-NAFLD patients receiving a 72-week PEM regimen were retrospectively enrolled. Routine blood and body composition results were analyzed immediately before and at the end of the study period.ResultsPEM treatment significantly improved liver enzyme levels such as aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase, and gamma-glutamyl transferase, along with lipid profiles including triglyceride, total cholesterol, and low-density lipoprotein cholesterol. PEM did not have any detectable impact on body composition parameters. The factors of female, higher AST (≥ 46 U/L) and fat mass (≥ 31.9%), as well as lower soft lean mass (&lt; 61.6%), skeletal muscle mass (&lt; 36%), and skeletal muscle mass index (&lt; 6.9 kg/m2) were significantly associated with the treatment response status of a &gt; 30% decrease in ALT. All patients completed the treatment without any adverse effects.ConclusionsLong-term PEM treatment had a positive impact on liver enzymes and lipid profiles, but it did not result in significant changes in body composition among HTG-NAFLD patients. In predicting the response to PEM treatment, the evaluation of AST and body composition may be useful.
Relationship of postprandial fibroblast growth factor 21 with lipids, inflammation and metabolic dysfunction-associated fatty liver disease during oral fat tolerance test
Authors: Li Xiaolong , Zheng Kunjie , Liu Lifang , Zhang Tingxue , Gu Wei , Hou Xiaoyu , Geng Jianlin , Song Guangyao 
Published: May 2024

IntroductionMetabolic dysfunction-associated fatty liver disease (MAFLD) is closely associated with serum fibroblast growth factor (FGF) 21; however, previous studies have typically focused on the static fasting state, and the relationships between postprandial FGF21 levels, postprandial metabolic status, and MAFLD remain unclear. Therefore, we measured postprandial lipids, inflammatory factors, and FGF21 levels in MAFLD and further analyzed their relationship using an oral fat tolerance test (OFTT).Patients and methodsIn total, 103 non-diabetic adult volunteers, including 46 patients with MAFLD, were included in this study. All participants underwent the OFTT. Venous blood samples were collected at 0, 2, 4, and 6 h. Circulating total cholesterol (TC), triglyceride (TG), free fatty acid (FFA), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α), hypersensitive-C reactive protein(hs-CRP) and FGF21 were assessed.ResultsSerum FGF21 significantly increased in the fasting state (P &lt; 0.05) and showed a biphasic change of first decreasing and then increasing in MAFLD during the OFTT. The postprandial levels of TG, TC, LDL-C, FFA, IL-6, TNF-α and hs-CRP were significantly increased in MAFLD (P &lt; 0.05). After adjusting for multiple factors, the FGF21 incremental area under the curve (iAUC) was linearly correlated with the FFA iAUC, TG iAUC, and IL-6 iAUC (P &lt; 0.05) and was an independent factor for MAFLD (P &lt; 0.05, OR=1.403).ConclusionDyslipidemia and excessive inflammation in MAFLD are associated to FGF21 levels in the postprandial period. An abnormal postprandial FGF21 response may be an important mechanism of MAFLD.
The association between leptin, adiponectin levels and the ovarian reserve in women of reproductive age
Authors: Nikolettos Konstantinos , Vlahos Nikolaos , Pagonopoulou Olga , Nikolettos Nikos , Zikopoulos Konstantinos , Tsikouras Panagiotis , Kontomanolis Emmanouil , Damaskos Christos , Garmpis Nikolaos , Psilopatis Iason , Asimakopoulos Byron 
Published: May 2024

BackgroundReproduction ability requires a certain amount of body fat that is necessary for ovulation, menstruation and pregnancy. Fat tissue represents an endocrine organ with high metabolic activity as it produces adipokines such as leptin and adiponectin. Our aim is to examine potential associations between women of reproductive age’s ovarian reserves and their levels of leptin and adiponectin.Method74 women between 19 and 40 years of age consented to take part. Based on the patterns of their ovarian reserves, the women were divided into three main groups: women with adequate ovarian reserves (AOR - Group A, n=30), women with polycystic ovary syndrome (PCOS - Group B, n=31) and women with depleted ovarian reserves (DOR - Group C, n=13). Among these groups, several biochemical and demographic parameters were statistically compared.ResultsCompared to the other two groups, women with DOR had statistically higher age and follicle stimulation hormone (FSH) levels. For estradiol (E2) and thyroid-stimulating hormone (TSH), no statistically significant difference was seen between the groups. In addition, women with PCOS had higher body mass index (BMI), luteinizing hormone (LH), total testosterone (TT), 17 hydroxyprogesterone (17-OHP), dehydroepiandrosterone (DHEA), anti-Mullerian hormone (AMH), and antral follicle count (AFC) than the other two groups. In line with expectations, women with DOR also had lower levels of AMH and AFC than the other two groups. Women with PCOS had higher leptin levels than the other two groups, but there was no statistically significant difference. Women with PCOS had lower levels of adiponectin than the other groups, however the difference was not statistically significant.ConclusionThe way we classified women in our study according to their ovarian reserves is completely consistent with what has been published internationally. The ovarian reserve in women of reproductive age is not strongly correlated with leptin and adiponectin levels. For safe conclusions, more research including a greater number of samples is required.
Longitudinal assessment of plasma biomarkers for early detection of cognitive changes in subjective cognitive decline
Authors: Hsieh Cheng-Hao , Ko Chien-An , Liang Chih-Sung , Yeh Po-Kuan , Tsai Chia-Kuang , Tsai Chia-Lin , Lin Guan-Yu , Lin Yu-Kai , Tsai Ming-Chen , Yang Fu-Chi 
Published: May 2024

BackgroundIndividuals experiencing subjective cognitive decline (SCD) are at an increased risk of developing mild cognitive impairment and dementia. Early identification of SCD and neurodegenerative diseases using biomarkers may help clinical decision-making and improve prognosis. However, few cross-sectional and longitudinal studies have explored plasma biomarkers in individuals with SCD using immunomagnetic reduction.ObjectiveTo identify plasma biomarkers for SCD.MethodsFifty-two participants [38 with SCD, 14 healthy controls (HCs)] underwent baseline assessments, including measurements of plasma Aβ42, Aβ40, t-tau, p-tau, and α-synuclein using immunomagnetic reduction (IMR) assays, cognitive tests and the Mini-Mental State Examination (MMSE). Following initial cross-sectional analysis, 39 individuals (29 with SCD, 10 HCs) entered a longitudinal phase for reassessment of these biomarkers and the MMSE. Biomarker outcomes across different individual categories were primarily assessed using the area under the receiver operating characteristic (ROC) curve. The SCD subgroup with an MMSE decline over one point was compared to those without such a decline.ResultsHigher baseline plasma Aβ1-42 levels significantly discriminated participants with SCD from HCs, with an acceptable area under the ROC curve (AUC) of 67.5% [95% confidence interval (CI), 52.7–80.0%]. However, follow-up and changes in MMSE and IMR data did not significantly differ between the SCD and HC groups (p &gt; 0.05). Furthermore, lower baseline plasma Aβ1-42 levels were able to discriminate SCD subgroups with and without cognitive decline with a satisfied performance (AUC, 75.0%; 95% CI, 55.6–89.1%). At last, the changes in t-tau and Aβ42 × t-tau could differentiate between the two SCD subgroups (p &lt; 0.05).ConclusionBaseline plasma Aβ42 may help identify people with SCD and predict SCD progression. The role of plasma Aβ42 levels as well as their upward trends from baseline in cases of SCD that progress to mild cognitive impairment and Alzheimer’s disease require further investigation.
Ultra-high pressure treatment improve the content of characteristic aromatic components of melon juice from the view of physical changes
Authors: Liu Xiao , Liang Feng , Wang Bing Su , Ren Fei Yue , Wang Wei , Zhang Chao 
Published: May 2024

IntroductionThe effectiveness of ultra-high pressure (UHP) technology in retaining the flavor of fresh fruit and vegetable juices has been acknowledged in recent years. Along with previously hypothesized conclusions, the improvement in melon juice flavor may be linked to the reduction of its surface tension through UHP.MethodsIn this paper, the particle size, free-water percentage, and related thermodynamic parameters of melon juice were evaluated in a physical point for a deeper insight.ResultsThe results showed that the UHP treatment of P2-2 (200 MPa for 20 min) raised the free water percentage by 7,000 times than the other treatments and both the melting enthalpy, binding constant and Gibbs free energy of P2-2 were minimized. This significantly increased the volatility of characteristic aromatic compounds in melon juice, resulting in a 1.2-5 times increase in the content of aromatic compounds in the gas phase of the P2-2 group compared to fresh melon juice.
Racial and survival disparities in inflammatory breast cancer (IBC) and non-IBC: a population-based study focused on Native Hawaiians and other Pacific Islanders
Authors: Yoshikawa Gene T. , Miyazaki Kyle SY. , Acoba Jared D. , Fujii Takeo 
Published: May 2024

BackgroundIt is well known that race is an independent predictor of breast cancer mortality and advanced stage at diagnosis. Inflammatory breast cancer (IBC) is the most aggressive type of breast cancer and has distinct clinical and biological features. Previous studies have shown that Blacks have a higher incidence of IBC than Whites. However, the proportion of IBC and the role of race on prognosis in Native Hawaiian and other Pacific Islander (NH/PI) populations with breast cancer are poorly understood. In this study, we aimed to examine the proportion of IBC to non-IBC in NH/PIs and to identify the clinicopathological, biological, and socioeconomic factors associated with the overall survival of NH/PIs compared to other races.MethodsUtilizing a comprehensive cancer registry from the largest hospital in Hawaii, newly diagnosed primary invasive breast cancer patients diagnosed between 2000 and 2018 were identified. Univariate and multivariate Cox proportional hazards models were used to test the association between race and clinical outcomes. Variables with P-values &lt;0.05 in the univariate analysis and race (variable of interest) were included in a multivariate analysis.ResultsThe cohort included 3691 patients, 60 of whom had IBC. NH/PI race had the highest proportion of IBC compared to other races (3.44%) but was not found to be an independent poor prognostic factor in IBC (HR 1.17 [95%CI 0.26-5.22]). Conversely, NH/PI race was associated with worse survival outcomes in patients with non-IBC (HR 1.65 [95%CI, 1.14-2.39]) along with other factors such as lack of insurance, underinsured status, triple-negative breast cancer (TNBC) subtype, age, and advanced clinical stage.ConclusionsThe findings of this study highlight that NH/PIs had higher rates of IBC and inferior survival in non-IBC compared to other races but not in IBC. It is essential to disaggregate NH/PI race from Asians in future population-based research studies. Further research is needed to understand the factors contributing to higher rates of IBC and poor survival outcomes in NH/PIs with non-IBC as well as targeted interventions to improve breast cancer outcomes in this population to ultimately help improve survival rates and reduce health inequities in NH/PIs with breast cancer.