bluuebunny/crossref_metadata_2025_split huggingface dataset:
Axoplasm isolation from peripheral nerve
Authors: Rishal Ida , Michaelevski Izhak , Rozenbaum Meir , Shinder Vera , Medzihradszky Katalin F. , Burlingame Alma L. , Fainzilber Mike 
Published: November 2009

AbstractLocalized changes in the composition of axonal cytoplasm (axoplasm) are critical for many biological processes, including axon guidance, responses to injury, neurite outgrowth, and axon‐glia interactions. Biochemical and molecular studies of these mechanisms have been heavily focused on in vitro systems because of the difficulty of obtaining subcellular extracts from mammalian tissues in vivo. As in vitro systems might not replicate the in vivo situation, reliable methods of axoplasm extraction from whole nerve would be helpful for mechanistic studies on axons. Here we develop and evaluate a new procedure for preparation of axoplasm from rat peripheral nerve, based on incubation of separated short segements of nerve fascicles in hypotonic medium to separate myelin and lyse nonaxonal structures, followed by extraction of the remaining axon‐enriched material. We show that this new procedure reduces serum and glial cell contamination and facilitates proteomic analyses of axonal contents. © 2009 Wiley Periodicals, Inc. Develop Neurobiol, 2010
Topography of estradiol‐modulated genomic responses in the songbird auditory forebrain
Authors: Sanford Sara E. , Lange Henry S. , Maney Donna L. 
Published: November 2009

AbstractSex steroids facilitate dramatic changes in behavioral responses to sociosexual signals and are increasingly implicated in the sensory processing of those signals. Our previous work demonstrated that in female white‐throated sparrows, which are seasonal breeders, genomic responses in the auditory forebrain are selective for conspecific song over frequency‐matched tones only when plasma estradiol (E2) reaches breeding levels. Here, we sought to map this E2‐dependent selectivity in the best‐studied area of the auditory forebrain, the caudomedial nidopallium (NCM). Nonbreeding females with low endogenous levels of E2 were treated with E2 or a placebo and exposed to conspecific song, tones, or no sound playback. Immunoreactive protein product of the immediate early gene zenk (egr‐1) was then quantified within seven distinct subregions, or domains, of NCM. We report three main findings: (1) regardless of hormone treatment, the zenk response is significantly higher in dorsal than in ventral NCM, and higher in medial than in lateral NCM; (2) E2‐dependent selectivity of the response is limited to the rostral and medial domains of NCM; in the more caudal domains, song induces more zenk expression than tones regardless of hormone treatment; (3) even when no sound stimuli were presented, E2 treatment significantly increased zenk expression in the rostral, but not the caudal, domains of NCM. Together, the latter two findings suggest that E2‐dependent plasticity in NCM is concentrated in rostral NCM, which is hodologically and neurochemically distinct from caudal NCM. Activity in rostral NCM may therefore be seasonally regulated in this species. © 2009 Wiley Periodicals, Inc. Develop Neurobiol 2010
A Mild, Efficient Approach for the Synthesis of 1,5‐Disubstituted Hydantoins
Authors: Olimpieri Francesca , Bellucci Maria Cristina , Volonterio Alessandro , Zanda Matteo 
Published: November 2009

AbstractAn efficient and straightforward two‐step procedure for the synthesis of N‐1 alkyl/aryl‐substituted hydantoins was developed, starting from easily available starting materials. The procedure envisages a highly regiospecific domino condensation/aza‐Michael (nucleophilic substitution)/O→N acyl migration between activated α,β‐unsaturated carboxylic acids or α‐haloaryl acetic acids, respectively, and N‐tert‐butyl‐ or N‐tritylcarbodiimides, leading to the regioselective formation of hydantoins bearing the tertiary alkylic substituent in the 3‐position, followed by selective removal the substituent. This process avoids the use of harsh reaction conditions and toxic reagents and is high yielding. A detailed study of the influence of the structure of the reactants on the reaction outcome is presented. A wide variety of final products having a primary, secondary, cyclic and aryl substituent at the N‐1 position were successfully synthesized by this method. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Platinum‐Catalyzed Hydrogenative Cyclization of Yne‐Enones, Yne‐Aldehydes, and Yne‐Dienes
Authors: Shinde Mahadev P. , Wang Xi , Kang Eun Joo , Jang Hye‐Young 
Published: November 2009

AbstractPt complexes were used in the presence of phosphane ligands, SnCl2, and H2 for coupling reactions of alkynes with electrophiles under environmentally benign hydrogenation conditions, providing five‐ and six‐membered cycloreduction products in moderate to good yield. Deuterium labeling studies suggested the occurrence of a catalytic cycle including the diploar intermediate derived from hydrometalation of a platinum–hydride complex to the alkyne. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Filmy channel microchip with amperometric detection
Authors: Wang Wei , Fu Feng F u , Xu Xueqin , Lin Jin‐Ming , Chen Guonan 
Published: November 2009

AbstractIn this article, a new type of microchip with filmy channels and a sample‐injection fracture is introduced. Unlike commercial microchip, new microchip possessed filmy channel with width 2–3 mm. The effective cooling ability made filmy channel microchip restrain the generation of Joule heat even under electric field of 588 V/cm. Moreover, wider channel could be more easily modified to prevent the absorption of samples, load more samples and result in a higher sensitivity. Sample‐injection fracture was first applied to match the filmy channel in microchip. Equipped with an amperometric detector, the characteristics of the newly designed filmy channel microchip had been studied and the results showed that it had good reproducibility, higher sensitivity and excellent separation ability. The microchip was also applied to separate L‐tryptophan's metabolites, namely 5‐hydroxy‐L‐tryptophan, 5‐hydroxytryptamine and 5‐hydroxy‐indole‐3‐acetic acid.
Functionalized carbon nanotubes as the pseudostationary phase for capillary EKC separation of non‐steroidal anti‐inflammatory drugs
Authors: Huang Yi‐Jin , Wang Guan‐Ren , Huang Kuan‐Pin , Hsieh Yu‐Fang , Liu Chuen‐Ying 
Published: November 2009

AbstractFunctionalized multiwalled carbon nanotubes (f‐MWCNTs) can serve as the pseudostationary phase (PSP) for the capillary EKC separation of non‐steroidal anti‐inflammatory drugs (NSAIDs). To increase their hydrophilicity, we treated MWCNTs, with a sonochemical process in a concentrated nitric/sulfuric acid mixture. The oxidized MWCNTs were then characterized by FT‐IR, transmission electron microscopy, and X‐ray photoelectron spectroscopy. We evaluated the potential of the PSP and the effects of buffer composition, pH, addition of organic modifier, and injection temperature on the NSAID separation. The PSP created a network structure of π–π interactions, hydrophobic forces, hydrogen bonding, and electrostatic interactions to separate NSAIDs, providing a different separation mode from SDS micelles. We achieved complete separation of six NSAIDs using a mixture of a borate buffer (75 mM, pH 10) with methanol (5%, v/v) containing 0.02 mg/mL f‐MWCNTs, an applied voltage of +12 kV and detection at 214 nm. Better precision was obtained with a low injection temperature. The method was also satisfactorily applied to the analysis of NSAIDs spiked into a urine sample.
CE‐ESI‐MS analysis of singly charged inorganic and organic anions using a dicationic reagent as a complexing agent
Authors: Lin Xiuli , Gerardi Anthony R. , Breitbach Zachary S. , Armstrong Daniel W. , Colyer Christa L. 
Published: November 2009

AbstractA dicationic ion‐pairing reagent, N,N′‐dibutyl 1,1′‐pentylenedipyrrolidinium, was used to form complexes with singly charged anions for their subsequent analysis by CE‐ESI‐MS in positive ion mode. This methodology offers the advantages of greater versatility and sensitivity relative to direct detection of the anions in negative ion mode, and it can be realized by a number of possible complexation strategies, including pre‐column, on‐column, and post‐column modes. Four model anions, perfluorooctanoate, benzenesulfonate (BZSN), monochloroacetate (MCA), and trifluoromethanesulfonimide were amenable to complexation with the dicationic reagent, yielding singly charged cations with greater m/z. By optimizing various parameters, including the CE separation buffer composition and pH, the concentration of the dicationic reagent, the mode of complexation, the nebulizing gas pressure, and the sheath liquid composition, it was possible to develop a robust CE‐ESI‐MS method appropriate for the analysis of anions in a tap water sample. By this method, LODs were found to be 20.9 and 1.31 ng/mL for MCA and BZSN, respectively.
Chirally functionalized anion‐exchange type silica monolith for enantiomer separation of 2‐aryloxypropionic acid herbicides by non‐aqueous capillary electrochromatography
Authors: Buchinger Stefanie , Follrich Beatrix , Lämmerhofer Michael , Lubda Dieter , Lindner Wolfgang 
Published: November 2009

AbstractA silica based monolithic capillary column derivatized with O‐9‐(tert‐butylcarbamoyl)quinidine was prepared for CEC enantiomer separation of chiral 2‐aryloxypropionic acid herbicides including inter alia dichlorprop, mecoprop and fenoprop. The silica monolith had a relatively low surface coverage with chiral cationic selector moieties. Due to the low selector density retention factors were low as well, yet still enabling enantiomer separations of the target solutes. Both electrophoretically and chromatographically dominated migration and separation modes, respectively, could be established depending on the employed conditions. In the former mode, enantiomers migrated in front of the EOF marker, and faster separations and higher plate numbers could be achieved. In the latter mode, stronger adsorption translated into a typical chromatographic separation in which the enantiomers eluted after the EOF marker whereby separation factors were slightly enhanced compared to the aforementioned separation mode. Reasonable baseline separations of enantiomers were accomplished for all analytes after optimization of relevant mobile phase parameters in the anion‐exchange CEC system including sample loadability, and the separations were comparable to such obtained on an optimized high density quinidine‐carbamate modified organic polymer monolith column. Overall, it is concluded that monoliths with a high surface density of chiral ion‐exchange moieties are favorable because of their enhanced sample loadabilities and improved chromatographic performance with regard to separation factors, plate numbers and peak symmetries. The resultant accompanying longer analysis times may rather be reduced by adjusting effective column length than by reducing selector coverage of the monolith.
Analyses of sulfonamide antibiotics by CEC using poly(divinylbenzene‐1‐octadecene‐vinylbenzyl trimethyl ammonium chloride) monolithic columns
Authors: Lin Cheng‐Lan , Cheng Yi‐Jie , Huang Hsi‐Ya , Lee Szetsen 
Published: November 2009

AbstractIn this study, a series of novel polymeric monolithic columns prepared by single‐step in situ copolymerization of 1‐octadecene (OD), divinylbenzene (DVB) and/or styrene (S), were developed as separation columns for the sulfonamide analyses. On the CEC method, the composition of monomer mixtures (i.e. the ratio of S versus OD), content of charge‐bearing monomer (vinylbenzyl trimethylammonium chloride) and volume fraction ratio of ACN in the mobile phase, was found to be the predominant influences for sulfonamide separation. Furthermore, an online sample concentration step, field‐amplified sample injection, was used to enhance the detection sensitivity of sulfonamides. Sample matrix's pH had a significant effect on the sulfonamide sensitivity. For the eight sulfonamides, the proposed poly(DVB‐OD) monolithic stationary phase coupled with field‐amplified sample injection step could achieve a reproducible baseline separation within 15 min and LODs in the range of 8.1–28.2 μg/L.
Noncovalent poly(1‐vinylpyrrolidone)‐based copolymer coating for the separation of basic proteins and lipoproteins by CE
Authors: Wang Ai‐Jun , Witos Joanna , D'Ulivo Lucia , Vainikka Kati , Riekkola Marja‐Liisa 
Published: November 2009

AbstractA new simple and fast noncovalent coating method based on poly(1‐vinylpyrrolidone‐co‐2‐dimethylaminoethyl methacrylate) copolymer was developed for CE. Merely 2 min flushing of the capillary with the poly(1‐vinylpyrrolidone‐co‐2‐dimethylaminoethyl methacrylate) copolymer was required. The copolymer is adsorbed onto the fused‐silica surface by hydrogen bonding and electrostatic interactions. EOF was almost totally suppressed over a wide pH range. The coating conditions (flushing time, copolymer concentration, and the concentration and pH of background electrolyte solution) and the stability of the coating were optimized, and the coated capillary was successfully applied to the fast separation of four basic proteins: lysozyme, cytochrome c, ribonuclease A, and α‐chymotrypsinogen A. Separation efficiencies were high, ranging from 386 000 to 738 000 plates/m at 40 mM pH 4.0 acetate buffer being comparable to values obtained on classical covalent PVP‐coated capillary. The RSD of migration times of basic proteins for 200 times successive runs were all below 1.0% (n=200, 3 days). A successful capillary performance was demonstrated also to the separation of low‐ and high‐density lipoproteins at acidic pH.
Surfactant‐bound monolithic columns for CEC
Authors: Gu Congying , He Jun , Jia Jinping , Fang Nenghu , Shamsi Shahab A. 
Published: November 2009

AbstractA novel anionic surfactant bound monolithic stationary phase based on 11‐acrylaminoundecanoic acid is designed for CEC. The monolith possessing bonded undecanoyl groups (hydrophobic sites) and carboxyl groups (weak cationic ion‐exchange sites) were evaluated as a mixed‐mode stationary phase in CEC for the separation of neutral and polar solutes. Using a multivariate D‐optimal design the composition of the polymerization mixture was modeled and optimized with five alkylbenzenes and seven alkyl phenyl ketones as test solutes. The D‐optimal design indicates a strong dependence of electrochromatographic parameters on the concentration of 11‐acrylaminoundecanoic acid monomer and porogen (water) in the polymerization mixture. A difference of 6, 8 and 13% RSD between the predicted and the experimental values in terms of efficiency, resolution and retention time, respectively, indeed confirmed that the proposed approach is practical. The physical (i.e. morphology, porosity and permeability) and chromatographic properties of the monolithic columns were thoroughly investigated. With the optimized monolithic column, high efficiency separation of N‐methylcarbamates pesticides and positional isomers was successfully achieved. It appears that this type of mixed‐mode monolith (containing both chargeable and hydrophobic sites) may have a great potential as a new generation of CEC stationary phase.
Nuclear factor‐κB expression as a novel marker of radioresistance in early‐stage laryngeal cancer
Authors: Yoshida Kenji , Sasaki Ryohei , Nishimura Hideki , Okamoto Yoshiaki , Suzuki Yoko , Kawabe Tetsuya , Saito Miki , Otsuki Naoki , Hayashi Yoshitake , Soejima Toshinori , Nibu Kenichi , Sugimura Kazuro 
Published: November 2009

AbstractBackground.The aim of this study was to evaluate the significance of nuclear factor‐kappa B (NF‐κB) expression as a marker of radioresistance in early‐stage laryngeal cancer.Methods.Thirty‐five patients with local recurrence and 70 case‐matched patients without local recurrence were entered in this study. NF‐κB expression was compared with Bcl‐2 and epidermal growth factor (EGF) receptor expression by immunohistochemistry, using pretreatment biopsy specimens. The prognostic value of NF‐κB was also evaluated. Twenty‐nine recurrent tumors were compared with pretreatment tumors.Results.NF‐κB expression in pretreatment tumors significantly correlated with local tumor control (p = .01), but bcl‐2 and EGF receptor expression did not. Only NF‐κB expression showed prognostic significance for local tumor control in both univariate and multivariate analyses (p = .008 and .04, respectively). NF‐κB expression was markedly enhanced in 23 of 29 (80%) recurrent tumors.Conclusion.NF‐κB expression may be a novel marker of radioresistance in early‐stage laryngeal cancer. © 2009 Wiley Periodicals, Inc. Head Neck, 2010
Anaplastic thyroid cancer: Clinical outcomes with conformal radiotherapy
Authors: Bhatia Aarti , Rao Archana , Ang Kie‐Kian , Garden Adam S. , Morrison William H. , Rosenthal David I. , Evans Douglas B. , Clayman Gary , Sherman Steven I. , Schwartz David L. 
Published: November 2009

AbstractBackground.The aim of this study was to review institutional outcomes for anaplastic thyroid cancer treated with conformal 3‐dimensional radiotherapy (3DRT) or intensity‐modulated radiotherapy (IMRT).Methods.In all, 53 consecutive patients were analyzed. Thirty‐one (58%) patients were irradiated with curative intent. Median radiation dose was 55 Gray (Gy; range, 4–70 Gy). Thirteen (25%) patients received IMRT to a median 60 Gy (range, 39.9–69.0 Gy). Thirty‐nine (74%) patients received chemotherapy with radiation.Results.The Kaplan–Meier estimate of overall survival (OS) at 1 year for definitively irradiated patients was 29%. Patients without distant metastases receiving ≥50 Gy had superior survival outcomes; 5 such patients had no evidence of disease at last follow‐up. Use of IMRT versus 3DRT did not influence toxicity.Conclusions.Outcomes for anaplastic thyroid cancer treated with 3DRT or IMRT remain equivalent to historical results. Healthy patients with localized disease who tolerate full dose irradiation can potentially enjoy prolonged survival. Biologically targeted radiosensitization merits prioritized investigation. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Kimura's disease in a white man
Authors: Müller‐Richter Urs D. A. , Moralis Antonios , Reuther Tobias , Kochel Michael , Reichert Torsten E. , Driemel Oliver 
Published: November 2009

AbstractBackgroundKimura's disease is a rare inflammatory disease that mainly affects Asians and most often occurs in the deep lymph nodes of the head and neck. We report on a rare case of Kimura's disease in the hard palate of a white man.MethodA 56‐year‐old white man was seen with a rapidly growing mass in the upper jaw. A complete tumor resection with hemimaxillectomy was performed. The tumor, which showed signs of inflammation, was located within the bone and the soft tissue.ResultsKimura's disease was diagnosed by histopathologic examination of the resected tumor.ConclusionThis case demonstrates that Kimura's disease, though rare, is not limited to the Asian population. We present a case of a tumor in a white man. This adds another possibility for uncertain differential diagnoses of rapidly growing tumor masses. © 2009 Wiley Periodicals, Inc. Head Neck 33:138–140, 2011
Accelerated fractionation radiotherapy and late intensification with 2 intra‐arterial cisplatin infusions for locally advanced head and neck squamous cell carcinoma
Authors: Yu Kwok Hung , Yu Simon C. H. , Hui Edwin P. , Kam Michael K. M. , Vlantis Alexander C. , Yuen Edmund , Chan Anthony T. C. 
Published: November 2009

AbstractBackgroundThis study was established to determine the maximum tolerated dose of intra‐arterial cisplatin (IAC) concurrent with accelerated fractionation radiotherapy for locally advanced head and neck squamous cell carcinoma (HNSCC).MethodsWe conducted a phase I study. Treatment consisted of 70 Gy/35 fractions/5.8 weeks and 2 weekly IAC infusions during the last 2 weeks.ResultsTen patients were recruited. Two patients had stage III, 1 had stage IVa, and 7 had stage IVb disease. Three patients received IAC at 100 mg/m2, 3 at 125 mg/m2, and 4 at 150 mg/m2. Nine patients received both planned infusions. Dose‐limiting toxicity occurred at 150 mg/m2 as transient grade 4 leukopenia and prolonged grade 3 acute skin reactions. The maximum tolerated dose was 125 mg/m2. Six patients survived disease‐free at 39 to 67 months.ConclusionsIt was feasible to give IAC concurrent with accelerated fractionation radiotherapy for locally advanced HNSCC. The maximum tolerated dose of cisplatin was 125 mg/m2. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Advanced glycation end‐products activate extracellular signal‐regulated kinase via the oxidative stress‐EGF receptor pathway in renal fibroblasts
Authors: Chen San‐Cher , Guh Jinn‐Yuh , Hwang Chi‐Ching , Chiou Shean‐Jaw , Lin Tai‐Du , Ko Yu‐Ming , Huang Jau‐Shyang , Yang Yu‐Lin , Chuang Lea‐Yea 
Published: November 2009

AbstractAdvanced glycation end‐products (AGEs), epidermal growth factor receptor (EGFR), reactive oxygen species (ROS), and extracellular signal‐regulated kinases (ERK) are implicated in diabetic nephropathy (DN). Therefore, we asked if AGEs‐induced ERK protein phosphorylation and mitogenesis are dependent on the receptor for AGEs (RAGE)–ROS–EGFR pathway in normal rat kidney interstitial fibroblast (NRK‐49F) cells. We found that AGEs (100 µg/ml) activated EGFR and ERK1/2, which was attenuated by RAGE short‐hairpin RNA (shRNA). AGEs also increased RAGE protein and intracellular ROS levels while RAGE shRNA and N‐acetylcysteine (NAC) attenuated AGEs‐induced intracellular ROS. Hydrogen peroxide (5–25 µM) increased RAGE protein level while activating both EGFR and ERK1/2. Low‐dose hydrogen peroxide (5 µM) increased whereas high‐dose hydrogen peroxide (100 µM) decreased mitogenesis at 3 days. AGEs‐activated EGFR and ERK1/2 were attenuated by an anti‐oxidant (NAC) and an EGFR inhibitor (Iressa). Moreover, AGEs‐induced mitogenesis was attenuated by RAGE shRNA, NAC, Iressa, and an ERK1/2 inhibitor (PD98059). In conclusion, it was found that AGEs‐induced mitogenesis is dependent on the RAGE–ROS–EGFR–ERK1/2 pathway whereas AGEs‐activated ERK1/2 is dependent on the RAGE–ROS–EGFR pathway in NRK‐49F cells. J. Cell. Biochem. 109: 38–48, 2010. © 2009 Wiley‐Liss, Inc.
The nucleosome binding protein HMGN3 is expressed in pancreatic α‐cells and affects plasma glucagon levels in mice
Authors: Kurahashi Toshihiro , Furusawa Takashi , Ueda Tetsuya , Bustin Michael 
Published: November 2009

AbstractGlucose homeostasis requires the coordinated actions of various organs and is critically dependent on the proper functioning of the various cell types present in the pancreatic Langerhans islets. Here we report that chromatin architectural protein HMGN3 is highly expressed in all pancreatic endocrine islet cells, and that Hmgn3−/− mice which have a mild diabetic phenotype, have reduced glucagon levels in their blood. To elucidate the mechanism leading to altered glucagon secretion of Hmgn3−/− mice, we tested whether HMGN3 affect glucagon synthesis and secretion in αTC1‐9 cells, a glucagon secreting cell line that is used to study pancreatic α‐cell function. We find that in these cells deletion of either HMGN3 or other HMGN variants, does not significantly affect glucagon gene expression or glucagon secretion. Our studies demonstrate a link between HMGN3 and glucagon blood levels that is not directly dependent of the function of pancreatic α‐cells. J. Cell. Biochem. 109: 49–57, 2010. Published 2009 Wiley‐Liss, Inc.