bluuebunny/crossref_metadata_2025_split huggingface dataset:
Overexpression of GOLPH3 Promotes Proliferation and Tumorigenicity in Breast Cancer via Suppression of the FOXO1 Transcription Factor
Authors: Zeng Zhaolei , Lin Huanxin , Zhao Xiaohui , Liu Guanglin , Wang Xi , Xu Ruihua , Chen Kun , Li Jun , Song Libing 
Published: June 2012

Abstract
Purpose: Golgi phosphoprotein 3 (GOLPH3) has been reported to be involved in various biologic processes. The clinical significance and biologic role of GOLPH3 in breast cancer, however, remains unknown.
Experimental Design: Expression of GOLPH3 in normal breast cells, breast cancer cells, and 6-paired breast cancer and adjacent noncancerous tissues were quantified using real-time PCR and Western blotting. GOLPH3 protein expression was analyzed in 258 archived, paraffin-embedded breast cancer samples using immunohistochemistry. The role of GOLPH3 in breast cancer cell proliferation and tumorigenicity was explored in vitro and in vivo. Western blotting and luciferase reporter analyses were used to investigate the effect of GOLPH3 overexpression and silencing on the expression of cell-cycle regulators and FOXO1 transcriptional activity.
Results: GOLPH3 was significantly upregulated in breast cancer cells and tissues compared with normal cells and tissues. Immunohistochemical analysis revealed high expression of GOLPH3 in 133 of 258 (51.6%) breast cancer specimens. Statistical analysis showed a significant correlation of GOLPH3 expression with advanced clinical stage and poorer survival. Overexpression and ablation of GOLPH3 promoted and inhibited, respectively, the proliferation and tumorigenicity of breast cancer cells in vitro and in vivo. GOLPH3 overexpression enhanced AKT activity and decreased FOXO1 transcriptional activity, downregulated cyclin-dependent kinase (CDK) inhibitor p21Cip1, p27Kip1, and p57Kip2, and upregulated the CDK regulator cyclin D1.
Conclusion: Our results suggest that high GOLPH3 expression is associated with poor overall survival in patients with breast cancer and that GOLPH3 overexpression increases the proliferation and tumorigenicity of human breast cancer cells. Clin Cancer Res; 18(15); 4059–69. ©2012 AACR.
The Prognostic Significance of Vasohibin-1 Expression in Patients with Upper Urinary Tract Urothelial Carcinoma
Authors: Miyazaki Yasumasa , Kosaka Takeo , Mikami Shuji , Kikuchi Eiji , Tanaka Nobuyuki , Maeda Takahiro , Ishida Masaru , Miyajima Akira , Nakagawa Ken , Okada Yasunori , Sato Yasufumi , Oya Mototsugu 
Published: June 2012

Abstract
Purpose: Vasohibin-1 (VASH1) is a novel angiogenic molecule that is specifically expressed in activated vascular endothelial cells, and the status of VASH1 expression has been documented in cancer angiogenesis. The aim of this study was to address the prognostic value of VASH1 expression in upper urinary tract urothelial carcinomas (UTUC).
Experimental Design: We retrospectively analyzed the clinical records of 171 patients with locally advanced UTUC (Ta-3N0M0). The median follow-up period was 3.8 years. We immunohistochemically examined the accomplished microvessels with anti-CD34 as microvessel density (MVD) and the microvessels with activated endothelial cells as VASH1 density. Then, we analyzed the association between immunohistochemical expression and clinical outcomes.
Results: Forty-two patients experienced tumor recurrence and of these 34 died of the disease during follow-up. VASH1 density was significantly associated with tumor grade, pathologic T stage, and MVD. The 5-year recurrence-free and cancer-specific survival rates were 66.1% and 72.8% in patients with VASH1 density (≥ 40/mm2) and 81.0% and 86.5% in their counterparts, respectively (P &lt; 0.05). MVD was not an independent predictor of tumor recurrence or cancer-specific survival. Multivariate analyses revealed that high VASH1 density was an independent prognostic indicator of both tumor recurrence (P = 0.024, HR = 2.10) and cancer-specific survival (P = 0.031, HR = 2.23) as well as other standard prognostic factors including high tumor grade and lymphovascular invasion.
Conclusions: VASH1 density represents a clinically relevant predictor of patient prognosis in UTUC. The results suggest that VASH1 density could become a new biomarker and provide additional prognostic information in patients with UTUC. Clin Cancer Res; 18(15); 4145–53. ©2012 AACR.
The kunitz protease inhibitor domain of protease nexin-2 inhibits factor XIa and murine carotid artery and middle cerebral artery thrombosis
Authors: Wu Wenman , Li Hongbo , Navaneetham Duraiswamy , Reichenbach Zachary W. , Tuma Ronald F. , Walsh Peter N. 
Published: June 2012

AbstractCoagulation factor XI (FXI) plays an important part in both venous and arterial thrombosis, rendering FXIa a potential target for the development of antithrombotic therapy. The kunitz protease inhibitor (KPI) domain of protease nexin-2 (PN2) is a potent, highly specific inhibitor of FXIa, suggesting its possible role in the inhibition of FXI-dependent thrombosis in vivo. Therefore, we examined the effect of PN2KPI on thrombosis in the murine carotid artery and the middle cerebral artery. Intravenous administration of PN2KPI prolonged the clotting time of both human and murine plasma, and PN2KPI inhibited FXIa activity in both human and murine plasma in vitro. The intravenous administration of PN2KPI into WT mice dramatically decreased the progress of FeCl3-induced thrombus formation in the carotid artery. After a similar initial rate of thrombus formation with and without PN2KPI treatment, the propagation of thrombus formation after 10 minutes and the amount of thrombus formed were significantly decreased in mice treated with PN2KPI injection compared with untreated mice. In the middle cerebral artery occlusion model, the volume and fraction of ischemic brain tissue were significantly decreased in PN2KPI-treated compared with untreated mice. Thus, inhibition of FXIa by PN2KPI is a promising approach to antithrombotic therapy.
The TWEAK/Fn14 pathway as an aggravating and perpetuating factor in inflammatory diseases; focus on inflammatory bowel diseases
Authors: Dohi Taeko , Burkly Linda C 
Published: June 2012

Abstract
Review on the TWEAK/Fn14 pathwayˈs emerging role in inflammatory diseases, with focus on IBD, and its interaction with other pathways to influence pathological processes.
The TWEAK/Fn14 pathway is a ligand/receptor pair of the TNFSF that has emerged as a prominent player in normal and pathological tissue remodeling. TWEAK/Fn14 pathway activation drives many processes relevant to autoimmune and inflammatory diseases. IBDs, including CD and UC, are chronic, relapsing inflammatory diseases of the GI tract. These diseases differ in their clinical, macroscopic, and histopathological presentation; however, pathological processes that prominently contribute, more or less in each case, include breakdown of the mucosal epithelial barrier, chronic inflammation, and tissue remodeling with fibrosis. TWEAK may promote the pathogenesis of IBD by signaling through Fn14, which can be up-regulated on IECs, thereby contributing to breakdown of the mucosal barrier; the induction of IEC-derived mediators that promote chronic inflammation and shape gut immunity against commensal flora; and delayed healing and fibrosis. TWEAK may also exert its action on endothelial and stromal cell types, including smooth muscle cells and fibroblasts, to promote chronic inflammation, dysregulated tissue repair, and fibrosis. Here, we review the data supporting an emerging role of the TWEAK/Fn14 pathway in autoimmune and inflammatory diseases, with a particular focus on IBD, and discuss how it interplays with other prominent pathways, including IL-13, TNF-α, and TGF-β, to aggravate and perpetuate the pathological processes underlying IBD.
Mechanism of radioprotection by δ-tocotrienol: pharmacokinetics, pharmacodynamics and modulation of signalling pathways
Authors: Satyamitra M , Ney P , Graves J , Mullaney C , Srinivasan V 
Published: June 2012

Objective
The objective of this study was to investigate the correlation between in vivo δ-tocotrienol (DT3) pharmacokinetics, pharmacodynamics and radiation protection, and to evaluate the effect of DT3 pre-treatment on radiation-induced alterations in apoptotic and autophagic pathways.

Methods
We evaluated pharmacokinetics (plasma, 0.5 to 12 h) and pharmacodynamics (peripheral blood indices; day 3, 7, 10 and 14) after a single subcutaneous injection of 300 mg kg−1 DT3 in unirradiated CD2F1 mice. Next, we monitored 30-day post-irradiation survival (9.25 Gy) and haematopoietic recovery of DT3-treated mice (7 Gy) exposed to cobalt-60 γ-irradiation. The effects of DT3 on irradiated bone marrow apoptosis and autophagy were determined by analyses of key caspases (3, 7, 9 and 8), beclin-1 and light chain 3 conversion.

Results
Plasma concentration of DT3 reached ∼195 µM (Cmax) 1 h after injection (Tmax), and DT3 was eliminated from plasma 12 h later. In unirradiated mice, DT3 significantly increased white blood cells (WBCs), neutrophils, lymphocytes (day 3 post DT3 injection) and platelets (day 7) by 1.5- to 2-fold, over vehicle-treated control. DT3 pre-treatment improved 30-day survival to 100% (∼15% in control) and accelerated recovery of reticulocytes, platelets, WBCs, neutrophils, lymphocytes and monocytes in peripheral blood. DT3 reduced activation of caspase-8, caspase-3 and caspase-7, inherent to apoptosis, while increasing autophagy-related beclin-1 expression in irradiated bone marrow.

Conclusion
These data indicate that DT3 stimulates multilineage haematopoiesis, protects against radiation-induced apoptosis downstream of the mitochondria and stimulates cytoprotective autophagy. Apart from a potent antioxidant activity, DT3 may elicit survival advantage following irradiation by enhancing haematopoiesis and modulating signalling pathways.
India and Sri Lanka's Civil War
Authors: Destradi Sandra 
Published: June 2012

Abstract
This article focuses on India's relationship with Sri Lanka in examining why a regional power failed to manage a conflict in its immediate neighborhood. Historical and domestic factors help explain India's largely hands-off policy (1991–2006). In contrast, regional and international factors underpin its support of Colombo's military campaign against the Liberation Tigers of Tamil Eelam, including New Delhi's concerns about China.
Low Temperature Plasma Synthesis of Nanocrystals and their Application to the Growth of Crystalline Silicon and Germanium Thin Films
Authors: Roca i Cabarrocas P. , Kim K.H. , Cariou R. , Labrune M. , Johnson E.V. , Moreno M. , Rios A. Torres , Abolmasov S. , Kasouit S. 
Published: June 2012

ABSTRACTWe summarize our research studies on the synthesis of silicon and germanium nanocrystals and their application to the growth of a variety of thin films, spanning the range from fully disordered amorphous up to fully ordered crystalline. All these films are deposited in a standard radio-frequency glow discharge system at low temperature (~200 °C). We show how the plasma synthesis of silicon nanocrystals, initially a side effect of powder formation, has become over the years an exciting field of research which has opened the way to new opportunities in the field of materials deposition and their application to optoelectronic devices. Our results suggest that epitaxy requires the melting/amorphization of the nanocrystals upon impact on the substrate, the subsequent epitaxial growth being favored on (100) c-Si substrates. As a consequence, the control of the impact energy is a critical aspect of the growth which will require new strategies such as the use of tailored voltage waveforms.
Terahertz light amplification by stimulated emission of radiation in optically pumped graphene
Authors: Otsuji Taiichi , Tombet Stephane Boubanga , Satou Akira , Chan S. , Ryzhii Victor 
Published: June 2012

ABSTRACTRecent advances in terahertz light amplification by stimulated emission of radiation in optically pumped graphene are reviewed. We present, within a picosecond time scale, fast relaxation and relatively slow recombination dynamics of photogenerated electrons and holes in an exfoliated graphene under infrared pulse excitation. We conduct time-domain spectroscopic studies using an optical pump and terahertz probe with an optical probe technique and show that graphene sheet amplifies an incoming terahertz field. The graphene emission spectral dependency on laser pumping intensity shows a threshold-like behavior, testifying to the occurrence of the negative conductivity and the population inversion. The emission spectra clearly narrow at a longer terahertz probe delay time, giving an evidence that the quasi-Fermi energy moves closer to the equilibrium at this longer terahertz probe delay time.
The Structure of Walking Activity in People After Stroke Compared With Older Adults Without Disability: A Cross-Sectional Study
Authors: Roos Margaret A. , Rudolph Katherine S. , Reisman Darcy S. 
Published: June 2012

Background
People with stroke have reduced walking activity. It is not known whether this deficit is due to a reduction in all aspects of walking activity or only in specific areas. Understanding specific walking activity deficits is necessary for the development of interventions that maximize improvements in activity after stroke.

Objective
The purpose of this study was to examine walking activity in people poststroke compared with older adults without disability.

Design
A cross-sectional study was conducted.

Methods
Fifty-four participants poststroke and 18 older adults without disability wore a step activity monitor for 3 days. The descriptors of walking activity calculated included steps per day (SPD), bouts per day (BPD), steps per bout (SPB), total time walking per day (TTW), percentage of time walking per day (PTW), and frequency of short, medium, and long walking bouts.

Results
Individuals classified as household and limited community ambulators (n=29) did not differ on any measure and were grouped (HHA-LCA group) for comparison with unlimited community ambulators (UCA group) (n=22) and with older adults without disability (n=14). The SPD, TTW, PTW, and BPD measurements were greatest in older adults and lowest in the HHA-LCA group. Seventy-two percent to 74% of all walking bouts were short, and this finding did not differ across groups. Walking in all categories (short, medium, and long) was lowest in the HHA-LCA group, greater in the UCA group, and greatest in older adults without disability.

Limitations
Three days of walking activity were captured.

Conclusions
The specific descriptors of walking activity presented provide insight into walking deficits after stroke that cannot be ascertained by looking at steps per day alone. The deficits that were revealed could be addressed through appropriate exercise prescription, underscoring the need to analyze the structure of walking activity.
Recruitment of Memory B Cells to Lymph Nodes Remote from the Site of Immunization Requires an Inflammatory Stimulus
Authors: Shenoy Gautam N. , Chatterjee Priyadarshini , Kaw Sheetal , Mukherjee Snigdha , Rathore Deepak K. , Bal Vineeta , Rath Satyajit , George Anna 
Published: June 2012

Abstract
Successful recall Ab responses require recruitment of quiescent memory B cells to secondary lymphoid organs. However, the cellular dynamics of memory cells responding to local antigenic challenge at lymphoid sites distal from the initial Ag encounter are not well understood. We show in this study that memory B cells generated following s.c. immunization in one footpad generate secondary responses to soluble Ag given i.p. but not to Ag given s.c. in the contralateral footpad unless LPS is coadministered. Memory B cells do not express CD62L, and CD62L−ve cells cannot enter lymph nodes unless LPS-mediated inflammation is induced there. Functional TLR4 is required on the B cells, as well as on non-B cells, in the lymph node to achieve full recruitment. Furthermore, splenectomized mice fail to respond to such inflammatory s.c. challenge in contralateral footpads, unlike lymphadenectomized mice lacking the original draining lymph nodes. Splenectomized mice also fail to respond to i.p. challenge with soluble Ag. Together, these data indicate that, unlike the central memory pool of T cells, which circulates through resting lymph nodes, the majority of long-lived memory B cells are spleen resident and require inflammatory signals for mounting recall responses at distal challenge sites.
Associations for change in physical and psychological factors and treatment response following exercise in knee osteoarthritis: An exploratory study
Authors: Fitzgerald G. Kelley , White Daniel K. , Piva Sara R. 
Published: June 2012

AbstractObjectiveUnderstanding how changes in physical and psychological factors following therapeutic exercise are associated with treatment outcome could have important implications for refining rehabilitation programs for knee osteoarthritis (OA). The objective of this study was to examine the association of changes in these factors with changes in pain and function after an exercise program for people with knee OA.MethodsIn total, 152 people with knee OA completed an exercise program consisting of lower extremity strengthening, stretching, range of motion, balance and agility, and aerobic exercises. The change from baseline to the 2‐month followup was calculated for physical and psychological factors, including self‐reported knee instability, quadriceps strength, knee and ankle range of motion, lower extremity muscle flexibility, fear of physical activity, anxiety, and depressive symptoms. Treatment response was defined as a minimum of a 20% improvement from baseline in both a numerical knee pain rating scale and the Western Ontario and McMaster Universities Osteoarthritis Index physical function scale. The association of each factor with treatment response was examined with logistic regression mutually adjusted for age, sex, body mass index, radiographic severity, and exercise group.ResultsChange in self‐reported knee instability (odds ratio [OR] 1.67 [95% confidence interval (95% CI) 1.13–2.47]) and fear of physical activity (OR 0.93 [95% CI 0.88–1.00]) were the only 2 factors that were significantly associated with treatment response after adjustment for covariates.ConclusionImprovement in knee instability and fear of physical activity were associated with increased odds of a positive treatment response following therapeutic exercise in subjects with knee OA.
ZnO/PVA Macroscopic Fibers Bearing Anisotropic Photonic Properties
Authors: Kinadjian Natacha , Achard Marie‐France , Julián‐López Beatriz , Maugey Maryse , Poulin Philippe , Prouzet Eric , Backov Rénal 
Published: June 2012

AbstractComposite PVA/ZnO‐nanorods fibers, synthesized through co‐axial flux extrusion exhibit higher anisotropic photonic properties, both in absorption and emission, as a result of the collective alignment of the ZnO nanorods along the main axis of the PVA fiber. This photonic anisotropy is triggered by a synergistic interaction between the PVA matrix, stretched above the glass transition temperature (Tg), and cooled down under strain. Compared with non‐elongated fibers that present an isotropic emission, composite fibers previously submitted to a tensile stress absorb selectively UV emission when the polarized laser beam is parallel to the main axis of the fiber. In addition, their photolumincescence is also anisotropic, with a waveguide behavior along the main axis of the fiber. Mechanical properties of these composite fibers are also drastically improved, compared with pure PVA fibers: the longitudinal Young modulus of these fibers is increased from 2 to 6 GPa upon ZnO addition, a value similar to those already observed for composite fibers, prepared either with carbon nanotubes, or V2O5 macroscopic fibers.
Successive Spray Deposition of P3HT/PCBM Organic Photoactive Layers: Material Composition and Device Characteristics
Authors: Abdellah Alaa , Virdi Kulpreet Singh , Meier Robert , Döblinger Markus , Müller‐Buschbaum Peter , Scheu Christina , Lugli Paolo , Scarpa Giuseppe 
Published: June 2012

AbstractControlling the active layer composition in organic electronic devices represents one of the major challenges in their fabrication. In particular, the composition of mixed donor/acceptor active layers for photosensitive device applications is known to strongly influence device performance. Here, an alternative approach for the preparation of organic heterojunction photoactive layers by successive spray deposition of the donor material, poly(3‐hexylthiophene) (P3HT), and acceptor material, [6,6]‐phenyl C61‐butyric acid methyl ester (PCBM), is reported. Optical absorption spectra, X‐ray reflectivity, and cross‐sectional transmission electron microscopy investigations are used to indicate the penetration of PCBM into a previously deposited P3HT layer and the spontaneous formation of a bulk heterojunction (BHJ) within the active layer, which provides the large interfacial area needed for efficient exciton dissociation. It is shown that organic photodiodes composed of photoactive layers prepared using this fabrication method exhibit a performance comparable to conventional BHJ devices in which the active layer is rigorously blended in advance. Moreover, separate handling of the individual materials and their deposition from distinct solutions enables an enhanced control of the active layer composition and hence increases the ability of tuning device characteristics.
Electrochemically Initiated Tandem and Sequential Conjugate Addition Processes: One‐Pot Synthesis of Diverse Functionalized Isoindolinones
Authors: Antico Pasqualmorica , Capaccio Vito , Di Mola Antonia , Massa Antonio , Palombi Laura 
Published: June 2012

AbstractA tandem aldol–heterocyclization–rearrangement reaction and one‐pot sequential Michael addition allowed a direct access to highly functionalized 3‐isoindolinones containing quaternary carbon centers. The desired products are obtained under mild conditions and in short reaction times by galvanostatic electrolysis in a divided cell. A further tandem intramolecular heterocyclization reaction leading to synthetically relevant hemiaminal derivatives has been established with suitable Michael acceptors.
Capillary Extrusion and Swell of a HDPE Melt Exhibiting Slip
Authors: Ansari Mahmoud , Mitsoulis Evan , Hatzikiriakos Savvas G. 
Published: June 2012

AbstractThe extrudate (die) swell of a high‐density polyethylene (HDPE) melt was studied both experimentally and numerically under slip conditions. The excess pressure drop due to entry (entrance pressure drop), the effect of pressure and temperature on viscosity, and the slip effects on the capillary data analysis have been examined. Using a series of capillary dies having different diameters,D, and length‐to‐diameterL/Dratios, a full rheological characterization has been carried out and the experimental data have been fitted both with a viscous model (Carreau–Yasuda) and a viscoelastic one (the Kaye‐Bernstein, Kearsley, Zapas/Papanastasiou, Scriven, Macosko or K‐BKZ/PSM model). Particular emphasis has been placed on the effects of wall slip (significant for HDPE). It was found that viscous modeling underestimates the pressures drops (especially at the higher apparent shear rates andL/Dratios) and predicts virtually no extrudate swell. On the other hand, the viscoelastic simulations were capable of reproducing the experimental data well, and this was particularly true for the pressure drop. The prediction of viscoelastic extrudate swell presented a problem, since the simulations grossly overpredict it due to the highly elastic nature of the melt. This occurs despite the presence of severe slip at the wall, which brings the swell down considerably. At this point it is not clear whether this is due to the viscoelastic model used or other phenomena, such as sagging and/or cooling, when simply extruding in the atmosphere. © 2012 Wiley Periodicals, Inc. Adv Polym Techn 32: E369–E385, 2013; View this article online atwileyonlinelibrary.com. DOI 10.1002/adv.21285