bluuebunny/crossref_metadata_2025_split huggingface dataset:
Takayasu arteritis presenting with large cerebral infarction in a 39-year-old Syrian woman: a case report
Authors: Muhammad Tareq , Hamdan Zulfiqar , Ebrahim Mohanad , Ali Basel , Hamdan Jafar 
Published: June 2024

Introduction and importance:
Takayasu Arteritis (TA) is a rare chronic inflammatory disease of unknown etiology that primarily affects large vessels, such as the aorta and its major branches. The disease typically presents with diverse symptoms, depending on the site and degree of arterial lesions. Delayed diagnosis is common, especially in younger populations.

Case presentation:
A 39-year-old Syrian female presented with an initial stroke. She had no prior medical history and was otherwise healthy. On examination, she had an absent left radial pulse, a carotid bruit, and muscle weakness. Blood tests showed an elevated ESR and CRP. Computed tomography of the brain revealed a right large cerebral infarction. Multislice computed tomography angiography showed diffuse arterial wall thickening, stenosis, and occlusion of several major vessels, including the left internal carotid artery, right internal carotid artery, and left subclavian artery.

Clinical discussion:
The patient was diagnosed with TA based on the American College of Rheumatology criteria. She was treated with prednisolone, methotrexate, and aspirin, and her symptoms improved significantly.

Conclusion:
This case highlights the importance of considering TA in the differential diagnosis of ischemic stroke, especially in young patients with atypical presentations. Early identification and management are essential to preclude critical sequelae.
Innovative techniques in the treatment of penile strangulation: a case report
Authors: Gazzah Wael , Mzoughi Mahdy , Lahouar Rayen , Najjai Yassine , Naouar Sahbi , Salem Braiek 
Published: June 2024

Introduction and importance:
Penile strangulation is a medical emergency characterized by the encirclement of the penis by an external object, resulting in circulatory compromise.

Case presentation:
A 35-year-old male presented with penile pain and urinary obstruction due to the inability to remove the ring. Upon examination, the ring was firmly lodged at the base of the penis, causing significant swelling and discoloration in the distal region.

Interventions and outcomes:
Initial attempts to cut the ring using standard tools were unsuccessful, leading to the engagement of a rescue team equipped with an air cutter. The cutting procedure, complicated by the ring’s thickness and hardness and the significant edema, took ~90 min. Safety measures, including the use of a surgical brain spatula and forceps, were employed to protect the penile skin from damage during the operation.

Relevance and impact:
This case underscores the necessity for timely intervention in penile strangulation cases and highlights the effectiveness of collaboration with specialized rescue teams equipped with appropriate cutting tools. It also emphasizes the importance of safety considerations when employing nonmedical devices in medical emergencies. The patient experienced a favorable outcome, with significant improvement in swelling and discoloration postprocedure, and no complications during follow-up. This report contributes to the limited but crucial literature on managing penile strangulation, particularly regarding the methods and timeframes for safely removing constricting objects.
Evaluating postoperative outcome assessment tools in hepato-pancreato-biliary surgery: A comparative analysis of the comprehensive complication index and Clavien–Dindo classification in a cohort of 1240 patients at a tertiary care center
Authors: Limbu Yugal , Acharya Bidur P. , Mishra Aakash , Regmee Sujan , Ghimire Roshan , Maharjan Dhiresh K. , Shrestha Suman K. , Thapa Prabin B. 
Published: June 2024

Introduction:
The comprehensive complication index (CCI) has emerged as a new tool for reporting postoperative complications. The aim of this study is to evaluate and compare the efficacy of CCI and Clavien–Dindo Classification (CDC) in measuring postoperative outcomes in patients undergoing hepato-pancreato-biliary (HPB) surgery.

Materials and methods:
In this single-centered, prospective, comparative study conducted between January 2022 and March 2023, 1240 patients underwent HPB surgery, including laparoscopic cholecystectomies and complex HPB surgery. Postoperative complications were evaluated utilizing the CCI and CDC indices, and their relationships with length of ICU stay, hospital stay, and return to activity were compared.

Results:
A total of 117 patients (9.44%) experienced complications of varying grades. There was a strong correlation between CCI and CDC (r=0.982, P <0.001). Both CCI and CDC demonstrated a strong correlation with the length of hospital stay, ICU stay, and return to normal activity. While CCI showed a better correlation with the length of hospital stay (r=0.706 vs. 0.695) and return to normal activity (r=0.620 vs. 0.611) than CDC, the difference was not statistically significant.

Conclusion:
CCI exhibited a stronger correlation with the length of stay and return to activity; however, no statistically significant advantage was observed over CDC.
Factors Affecting the Choice to Forgo Nipple Grafts in Gender-Affirming Chest Surgery
Authors: Ferrin Peter C. , Pua Ezra , Isbester Kelsey , Peters Blair R. 
Published: June 2024

Introduction
Some patients pursuing gender-affirming mastectomy prefer to forgo autologous nipple and areolar reconstruction, instead choosing a “no nipple” option. The objective of this study is to evaluate the motives and influences contributing to this decision.

Methods
A retrospective survey-based study of patients undergoing gender-affirming mastectomy over a 4.6-year period was conducted. All patients were offered nipple and areolar reconstruction. A survey was distributed to those who elected to forgo nipple and areolar reconstruction exploring the factors influencing that choice and postoperative satisfaction.

Results
Five hundred thirty-six patients underwent gender-affirming mastectomy and 13% chose to forgo nipple and areolar reconstruction. The survey response rate was 72%. Most respondents identified as nonbinary (48%) or transmasculine (42%). Body image, defined in the context of this study as an improvement in body image satisfaction due to achievement of a more gender-congruent appearance, was the most highly cited and most heavily weighted decision-making factor. Concerns about nipple graft outcomes were frequently cited as important but carried less weight. There were no differences between transmasculine and nonbinary patients in terms of motivations for choosing this surgical option. Fifty-eight percent of respondents pursued or planned to pursue chest tattoos, whereas 42% preferred no further chest modifications. Patient satisfaction was high postoperatively (98%), and 82% of respondents would choose to forgo nipple and areolar reconstruction again even if surgical outcomes of free nipple grafts were improved.

Conclusions
Gender-affirming mastectomy without nipple and areolar reconstruction was requested by 13% of patients presenting for gender-affirming chest surgery over the study period and had high postoperative satisfaction among patients who chose this option. This modification of gender-affirming mastectomy is a variation that surgical teams should be aware of. Many reasons to forgo nipple preservation were cited, most of which related to body image. Secondary factors were easier recovery and/or concern about outcomes of free nipple grafts. Many patients wished information about this surgical option was more widely available.
Comparison of eGFR Equations to Guide Dosing of Medications for Kidney Transplant Recipients
Authors: Akbari Ayub , El Wadia Hajar , Knoll Greg A. , White Christine A. , Sood Manish M. , Massicotte-Azarniouch David , McCudden Christopher , Deschenes Marie-Josee , Salman Maria , Ramsay Tim , Hundemer Gregory L. 
Published: June 2024

Background.
Clinicians caring for kidney transplant recipients (KTRs) most commonly use estimated glomerular filtration rate (eGFR) to guide medication dosing as it is the most readily available measure of kidney function. Which eGFR equations provide the most accurate medication dosing guidance for KTRs remains uncertain.

Methods.
We studied 415 stable KTRs in Canada and New Zealand. Participants completed same-day measurements of creatinine and cystatin C and measured GFR (diethylenetriaminepentaacetic acid). Chronic Kidney Disease Epidemiology Collaboration, European Kidney Function Consortium, and transplant-specific eGFR equations were compared with both Cockcroft-Gault creatinine clearance (CrCl) and measured GFR. eGFR equations were assessed both indexed to a standardized body surface area (BSA) of 1.73 m2 (milliliter per minute per 1.73 m2, as is conventional reporting from most clinical laboratories) and nonindexed (milliliter per minute) accounting for actual BSA. The primary outcome was the proportion of medication dosing discordance relative to Cockcroft-Gault CrCl or measured GFR for 8 commonly prescribed medications. Stratified analyses were performed on the basis of obesity status.

Results.
Nonindexed eGFR equations (milliliter per minute) resulted in substantially lower medication dosing discordance compared with indexed eGFR equations (milliliter per minute per 1.73 m2). These findings were most pronounced among KTRs with obesity, in whom underdosing was frequent. When compared with Cockcroft-Gault CrCl, the lowest proportion of discordance was found with the nonindexed 2023 transplant-specific equation. When compared with measured GFR, the lowest proportion of discordance was found with the nonindexed 2021 Chronic Kidney Disease Epidemiology CollaborationCr/CysC equation.

Conclusions.
Nonindexed eGFR values accounting for actual BSA should be used by clinicians for medication dosing in KTRs. These findings may inform KT providers about which eGFR equations provide the safest, most accurate medication dosing guidance for KTRs.
A protein kinase A-regulated centrosomal actin pool sets the threshold for T cell polarization
Authors: Simao Morgane , Régnier Fabienne , Randriamampita Clotilde 
Published: June 2024

AbstractT lymphocyte migration triggered by chemokine stimulation is preceded by cell polarization. The acquisition of this asymmetry requires a profound cell rearrangement, particularly of the cytoskeleton. The mechanism by which a uniform signal triggered by chemokine receptors rapidly leads to this asymmetry is largely elusive. Using cell imaging, we emphasize that the centrosome dictates the position of the polarization axis in T lymphocytes. Mechanistically, we highlight that the T cell shape is controlled by the amount of actin filaments surrounding the centrosome. In resting conditions as well as after chemokine stimulation, the activity of a specific pool of protein kinase A regulates this cytoskeleton compartment. Once the centrosomal actin is reduced below a certain threshold, the symmetry breaking is catalyzed. This study points to a critical protein kinase A signaling pathway in the establishment of the immune response.
A microcontroller-based approach to control oxygen and establish flexible acclimation regimes
Authors: Mucha Stefan 
Published: June 2024

AbstractEnvironmental control systems are important tools for experimental researchers studying animal-environment interactions. Commercial systems for measurement and regulation of environmental oxygen conditions are relatively expensive and can not always be adapted to varying experimental applications. Here, we present a low-cost and highly flexible oxygen control system using Arduino microcontrollers in combination with a commercial optical oxygen sensor. We show dissolved oxygen (DO) measurements from three experiments that were conducted with mormyrid fish with different DO regimes: 1) acute challenge with defined rates of DO change from normoxia (> 90% air saturation) to hypoxia (10% air saturation), 2) acute stepwise hypoxia in a shuttle-box setup, and 3) long-term hypoxia (15 % air saturation) that was maintained for eight weeks in the home tank of the fish. Overall, the microcontroller-based DO control systems produced the desired experimental conditions with a high and inter-trial correlation (Pearson’s R ≥ 0.987) and sufficient precision, except for the shuttle-box setup, where the lowest DO step of 10% air saturation could not be reached.
Engineered bacteria that self-assemble “bioglass” polysilicate coatings display enhanced light focusing
Authors: Sidor Lynn M. , Beaulieu Michelle M. , Rasskazov Ilia , Acarturk B. Cansu , Ren Jie , Kamoen Lycka , Vitali María Vázquez , Carney P. Scott , Schmidt Greg R. , Srubar Wil V. , Abbondanzieri Elio A. , Meyer Anne S. 
Published: June 2024

AbstractPhotonic devices are cutting-edge optical materials that produce narrow, intense beams of light, but their synthesis typically requires toxic, complex methodology. Here we employ a synthetic biology approach to produce environmentally-friendly, living microlenses with tunable structural properties. We engineeredEscherichia colibacteria to display the silica biomineralization enzyme silicatein from aquatic sea sponges. Our silicatein-expressing bacteria can self-assemble a shell of polysilicate “bioglass” around themselves. Remarkably, the polysilicate-encapsulated bacteria can focus light into intense nanojets that are nearly an order of magnitude brighter than unmodified bacteria. Polysilicate-encapsulated bacteria are metabolically active for up to four months, potentially allowing them to sense and respond to stimuli over time. Our data demonstrate that engineered bacterial particles have the potential to revolutionize the development of multiple optical and photonic technologies.Significance StatementIn this work, we apply the principles of synthetic biology to create living optical devices. Utilizing the ability of sea sponges to polymerize bioglass from silica precursors in the ocean water using only a single enzyme, silicatein, we have fused this same enzyme to the surface ofEscherichia colibacterial cells. The modified bacteria can polymerize a layer of bioglass at their surface. This bioglass shell allows the bacteria to act as engineered optical devices that are able to scatter high intensity, focused light while also surviving for several months, opening the door to a wide range of sense-and-respond applications.ClassificationBiological Sciences, Applied Biological Sciences
TRIM32 inhibits Venezuelan Equine Encephalitis Virus Infection by targeting a late step in viral entry
Authors: Xie Yifan , Cao Jie , Gan Shuyi , Xu Lingdong , Zhang Dongjie , Qian Suhong , Xu Feng , Ding Qiang , Schoggins John W. , Fan Wenchun 
Published: June 2024

AbstractAlphaviruses are mosquito borne RNA viruses that are a reemerging public health threat. Alphaviruses have a broad host range, and can cause diverse disease outcomes like arthritis, and encephalitis. The host ubiquitin proteasome system (UPS) plays critical roles in regulating cellular processes to control the infections with various viruses, including alphaviruses. Previous studies suggest alphaviruses hijack UPS for virus infection, but the molecular mechanisms remain poorly characterized. In addition, whether certain E3 ubiquitin ligases or deubiquitinases act as alphavirus restriction factors remains poorly understood. Here, we employed a cDNA expression screen to identify E3 ubiquitin ligase TRIM32 as a novel intrinsic restriction factor against alphavirus infection, including VEEV-TC83, SINV, and ONNV. Ectopic expression of TRIM32 reduces alphavirus infection, whereas depletion of TRIM32 with CRISPR-Cas9 increases infection. We demonstrate that TRIM32 inhibits alphaviruses through a mechanism that is independent of the TRIM32-STING-IFN axis. Combining reverse genetics and biochemical assays, we found that TRIM32 interferes with genome translation after membrane fusion, prior to replication of the incoming viral genome. Furthermore, our data indicate that the monoubiquitination of TRIM32 is important for its antiviral activity. Notably, we also show two TRIM32 pathogenic mutants R394H and D487N, related to Limb-girdle muscular dystrophy (LGMD), have a loss of antiviral activity against VEEV-TC83. Collectively, these results reveal that TRIM32 acts as a novel intrinsic restriction factor suppressing alphavirus infection and provides insights into the interaction between alphaviruses and the host UPS.Author summaryDue to climate change, wildlife habitat loss, and human activities, alphavirus infections are a growing threat to public health. The host UPS has critical role in virus-host interaction, but how the UPS impact alphavirus infection is not completely understood. In this study, we found that the E3 ubiquitin ligase TRIM32 inhibits diverse alphaviruses in multiple cell types. Mechanistically, TRIM32 impairs primary translation of incoming viral genome in a manner that depends on. monoubiquitination of TRIM32. Additionally, disease-associated alleles of TRIM32 have a loss-of-function with respect to viral inhibition. Together, these findings uncover a novel biological function of TRIM32 in regulating alphavirus infection and provide important insights into the interplay between alphaviruses and the host USP.
Measurement of the
e+e−→π+π−
cross section from threshold to 1.2 GeV with the CMD-3 detector
Authors: Ignatov F. V. , Akhmetshin R. R. , Amirkhanov A. N. , Anisenkov A. V. , Aulchenko V. M. , Bashtovoy N. S. , Berkaev D. E. , Bondar A. E. , Bragin A. V. , Eidelman S. I. , Epifanov D. A. , Epshteyn L. B. , Erofeev A. L. , Fedotovich G. V. , Gorkovenko A. O. , Grancagnolo F. J. , Grebenuk A. A. , Gribanov S. S. , Grigoriev D. N. , Ivanov V. L. , Karpov S. V. , Kasaev A. S. , Kazanin V. F. , Khazin B. I. , Kirpotin A. N. , Koop I. A. , Korobov A. A. , Kozyrev A. N. , Kozyrev E. A. , Krokovny P. P. , Kuzmenko A. E. , Kuzmin A. S. , Logashenko I. B. , Lukin P. A. , Lysenko A. P. , Mikhailov K. Yu. , Obraztsov I. V. , Okhapkin V. S. , Otboev A. V. , Perevedentsev E. A. , Pestov Yu. N. , Popov A. S. , Razuvaev G. P. , Rogovsky Yu. A. , Ruban A. A. , Ryskulov N. M. , Ryzhenenkov A. E. , Semenov A. V. , Senchenko A. I. , Shatunov P. Yu. , Shatunov Yu. M. , Shebalin V. E. , Shemyakin D. N. , Shwartz B. A. , Shwartz D. B. , Sibidanov A. L. , Solodov E. P. , Talyshev A. A. , Timoshenko M. V. , Titov V. M. , Tolmachev S. S. , Vorobiov A. I. , Zemlyansky I. M. , Zhadan D. S. , Zharinov Yu. M. , Zubakin A. S. , Yudin Yu. V. ,   CMD-3 Collaboration
Published: June 2024

The cross section of the process e+e−→π+π− has been measured in the center of mass energy range from 0.32 to 1.2 GeV with the CMD-3 detector at the electron-positron collider VEPP-2000. The measurement is based on a full dataset collected below 1 GeV during three data taking seasons, corresponding to an integrated luminosity of about 62  pb−1. In the dominant ρ-resonance region, a systematic uncertainty of 0.7% has been reached. At energies around ϕ-resonance the π+π− production cross section was measured for the first time with high beam energy resolution. The forward-backward charge asymmetry in the π+π− production has also been measured. It shows a strong deviation from the theoretical prediction based on the conventional scalar quantum electrodynamics framework, and it is in good agreement with the generalized vector-meson-dominance and dispersive-based predictions. The impact of the presented results on the evaluation of the hadronic contribution to the anomalous magnetic moment of muon is discussed.

Published by the American Physical Society
2024
Public perceived efficacy of emergency infrastructure project: antecedents in a triadic reciprocal determinism
Authors: Cui Caiyun , Xie Tingyu , Liu Yong , Liu Meng , Cao Huan , Li Huilian 
Published: June 2024

PurposeThis paper aims to explore the influencing factors of public perceived efficacy of emergency infrastructure projects based on the triadic interactive determinism, and analyze the relationship among these factors.Design/methodology/approachBased on the triadic interactive determinism, we explored the factors influencing public perceived efficacy of emergency infrastructure project and empirically verified the relationship among these factors and perceived efficacy by using data drawn from a questionnaire survey of 491 residents near Leishenshan Hospital, Jiangxia District, Wuhan, China.FindingsPrior experience, emotional response, personal expectation, public trust, context message and interactivity level, namely behavior, individual and environment, affect the perceived efficacy of public emergency infrastructure projects.Practical implicationsThe results offer an insight into public perceived efficacy of emergency infrastructure project from the perspective of antecedents in a triadic reciprocal determinism, which provides a reference basis for the sustainable development of the emergency infrastructure projects. This study also suggests valuable practical implications to government departments to improve the quality of administrative decision-making effectively.Originality/valueAlthough existing studies have found some influencing factors of public perceived efficacy in general infrastructure, there is still a lack of systematic carding and quantitative description of influencing factors of public perceived efficacy of emergency infrastructure projects. This study bridges this gap by exploring the determinants and their influencing relationship of public perceived efficacy especially for emergency infrastructure projects.