bluuebunny/crossref_metadata_2025_split huggingface dataset:
The selective VEGFR1‐3 inhibitor axitinib (AG‐013736) shows antitumor activity in human neuroblastoma xenografts
Authors: Rössler Jochen , Monnet Yann , Farace Francoise , Opolon Paule , Daudigeos‐Dubus Estelle , Bourredjem Abderrahmane , Vassal Gilles , Geoerger Birgit 
Published: August 2010

AbstractTumor angiogenesis in childhood neuroblastoma is an important prognostic factor suggesting a potential role for antiangiogenic agents in the treatment of high‐risk disease. Within the KidsCancerKinome project, we evaluated the new oral selective pan‐VEGFR tyrosine kinase inhibitor axitinib (AG‐013736) against neuroblastoma cell lines and the subcutaneous and orthotopic xenograft model IGR‐N91 derived from a primary bone marrow metastasis. Axitinib reduced cell proliferation in a dose‐dependent manner with IC50 doses between 274 and >10,000 nmol/l. Oral treatment with 30 mg/kg BID for 2 weeks in advanced tumors yielded significant tumor growth delay, with a median time to reach five times initial tumor volume of 11.4 days compared to controls (p = 0.0006) and resulted in significant reduction in bioluminescence. Simultaneous inhibition of VEGFR downstream effector mTOR using rapamycin 20 mg/kg q2d×5 did not statistically enhance tumor growth delay compared to single agent activities. Axitinib downregulated VEGFR‐2 phosphorylation resulting in significantly decreased microvessel density (MVD) and overall surface fraction of tumor vessels (OSFV) in all xenografts as measured by CD34 immunohistochemical staining (mean MVD ± SD and OSFV at 14 days 21.27 ± 10.03 in treated tumors vs. 48.79 ± 17.27 in controls and 0.56% vs. 1.29%; p = 0.0006, respectively). We further explored the effects of axitinib on circulating mature endothelial cells (CECs) and endothelial progenitor cells (CEPs) measured by flow cytometry. While only transient modification was observed for CECs, CEP counts were significantly reduced during and up to 14 days after end of treatment. Axitinib has potent antiangiogenic properties that may warrant further evaluation in neuroblastoma.
Fiber‐optic probes forin vivodepth‐resolved neuron‐activity mapping
Authors: Doronina‐Amitonova Lyubov V. , Fedotov Il'ya V. , Ivashkina Olga I. , Zots Marina A. , Fedotov Andrei B. , Anokhin Konstantin V. , Zheltikov Aleksei M. 
Published: August 2010

AbstractSpecialty fiber probes are used forin vivodepth‐resolved mapping of neuron activity through the optical detection of fluorescent‐protein reporters expressed inside the living brain of anesthetized transgenic mice. Supercontinuum radiation produced by highly nonlinear photonic‐crystal fibers is employed to demonstrate a simultaneous multicolor interrogation of several biomarkers in a model aqueous solution system, thus suggesting the way toward a multiplex mapping of various types of neuron dynamics inside the living brain. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Retrieving skin properties fromin vivospectral reflectance measurements
Authors: Yudovsky Dmitry , Pilon Laurent 
Published: August 2010

AbstractA previously developed inverse method was applied toin vivonormal‐hemispherical spectral reflectance measurements taken on the inner and outer forearm as well as the forehead of healthy white Caucasian and black African subjects. The inverse method was used to determine the thickness and melanin concentration in the epidermis, dermal blood volume fraction and oxygen saturation, and skin's spectral scattering coefficient. It was established that changes in melanin concentration due to racial difference and tanning, and differences in epidermal thickness and blood volume with anatomical location were detectable. The retrieved values were also consistent with independent measurements reported in the literature. The same method could be used for optical diagnosis of pathologies affecting the structure and pigmentation of human skin. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
High‐ and low‐frequency mechanical properties of living starfish oocytes
Authors: Pesce Giuseppe , Selvaggi Lara , Rusciano Giulia , Sasso Antonio 
Published: August 2010

AbstractWe studied the mechanical properties of living starfish oocytes belonging to two species, Astropecten Auranciacus and Asterina pectinifera, over a wide range of timescales. We monitored the Brownian motion of microspheres injected in the cytoplasm using laser particle‐tracking (LPT) and video multiple‐particle‐tracking (MPT) techniques, to explore high‐ and low‐frequency response ranges, respectively. The analysis of the mean‐square‐displacements (MSD) allowed us to characterize the samples on different timescales. The MSD behavior is explained by three power‐law exponents: for short times (τ < 1 ms) it reflects the semiflexible behavior of the actin network; for intermediate timescales (1 ms < τ < 1 s) it is similar to that of a soft‐glass material; finally for long times (τ > 1 s) it behaves mainly like a viscous medium. We computed and compared the viscoelastic moduli using a recently proposed model describing the frequency response of the cell material. The large fluctuations found in the MSD over hundreds of trajectories indicate and confirm the significant cytoplasm heterogeneity. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Gene expression, glycocalyx assay, and surface properties of human endothelial cells cultured on hydrogel matrix with sulfonic moiety: Effect of elasticity of hydrogel
Authors: Yang Jing Jing , Chen Yong Mei , Kurokawa Takayuki , Gong Jian Ping , Onodera Shin , Yasuda Kazunori 
Published: August 2010

AbstractWe measured the gene expression, glycocalyx content, and surface properties of human coronary artery endothelial cells (HCAECs) cultured on poly(sodium p‐styrene sulfonate) (PNaSS) hydrogels with various levels of elasticity ranged in 3–300 kPa. We found that all HCAECs reached confluence on these hydrogels while retaining the similar expression of EC‐specific markers to that on polystyrene (PS), a widely used scaffold in cell culture in vitro. Real‐time polymerase chain reaction (PCR) and glycosaminoglycan (GAG) assay showed that the amount of EC‐specific glycocalyx secreted by HCAECs cultured on PNaSS gels was higher than that cultured on PS, and it increased with an increase of gel elasticity. Furthermore, the HCAECs cultured on PNaSS gels showed excellent property against platelet adhesion and lower surface friction than that on PS. The platelet adhesion and surface friction of HCAECs cultured on PNaSS gels also depend on the elasticity of gels. The largest amount of EC‐specific glycocalyx, excellent blood compatibility, and the lowest friction were observed when the elastic modulus of the gel was larger than 60 kPa. Overall, HCAECs cultured on these hydrogels have better properties than those cultured on PS scaffold, demonstrating the PNaSS gels can be used as potential tissue engineering material for blood vessels. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Oncostatin M synergistically induces CXCL10 and ICAM‐1 expression in IL‐1β‐stimulated‐human gingival fibroblasts
Authors: Hosokawa Yoshitaka , Hosokawa Ikuko , Ozaki Kazumi , Nakae Hideaki , Matsuo Takashi 
Published: August 2010

AbstractPeriodontitis is a chronic bacterial infection of tooth‐supporting structures. T‐helper type 1 (Th1) cells are related to the exacerbation of periodontal disease. Human gingival fibroblasts (HGFs), the major cell type in periodontal connective tissues, are involved in immunological response in periodontal tissues. However, it is uncertain whether HGFs are related to Th1 response. Chemokine (C‐X‐C motif) ligand 10 (CXCL10) is a cytokine, that is related to Th1 cells migration. Intercellular adhesion molecule (ICAM)‐1 is involved in Th1 cells retention and activation in inflamed tissue. The aim of this study is to examine the effect of oncostatin M (OSM) on CXCL10 and ICAM‐1 expression in HGFs. OSM stimulation induced CXCL10 and ICAM‐1 expression in HGFs. Moreover, the synergistic effects of CXCL10 release and ICAM‐1 expression in HGFs were observed with combined stimulation of interleukin (IL)‐1β and OSM. OSM increased type 1 IL‐1 receptor (IL‐1R1) expression, and IL‐1β enhanced OSMRβ expression on HGFs. IL‐1β + OSM stimulation enhanced the phosphorylation of inhibitor of nuclear factor κB (IκB)‐α, signal transducer and activator of transcription (STAT)3, c‐Jun N terminal kinase (JNK), and protein kinase B (Akt) compared to OSM or IL‐1β stimulation. CXCL10 production from OSM + IL‐1β stimulated HGFs was suppressed by nuclear factor (NF)‐κB, STAT3, JNK, and phosphoinositide‐3‐kinase (PI3K) inhibitors. On the other hand, only NF‐κB and STAT3 inhibitors suppressed ICAM‐1 expression enhanced by OSM + IL‐1β treatment. These effects of OSM and IL‐1β may promote Th1 cells infiltration and retention in periodontally diseased tissues and be related to exacerbation of periodontal disease. J. Cell. Biochem. 111: 40–48, 2010. © 2010 Wiley‐Liss, Inc.
Prospective evaluation of a cognitive vulnerability‐stress model for depression: the interaction of schema self‐structures and negative life events
Authors: Seeds Pamela M. , Dozois David J.A. 
Published: August 2010

AbstractThis study tested the diathesis‐stress component of Beck's (1967) cognitive theory of depression. Initially, participants completed measures assessing cognitive organization of the self‐schema and depressive symptoms. One year later, participants completed measures assessing cognitive organization of the self‐schema, depressive symptoms, and negative life events. Hierarchical multiple regression analyses, controlling for initial depression, indicated that more tightly interconnected negative content was associated with greater elevations in depressive symptoms following the occurrence of life events. More diffusely interconnected positive content for interpersonal self‐referent information also interacted with life events to predict depressive symptoms. Cognitive organization dimensions showed moderate to high stability across the follow‐up, suggesting that they may be trait‐like vulnerability factors. Implications for the cognitive vulnerability‐stress model of depression are discussed. © 2010 Wiley Periodicals, Inc. J Clin Psychol 66:1–17, 2010.
Ultrastructure of spermiogenesis in the American alligator,Alligator mississippiensis(Reptilia, Crocodylia, Alligatoridae)
Authors: Gribbins Kevin M. , Siegel Dustin S. , Anzalone Marla L. , Jackson Daniel P. , Venable Katherine J. , Rheubert Justin L. , Elsey Ruth M. 
Published: August 2010

AbstractTesticular samples were collected to describe the ultrastructure of spermiogenisis inAlligator mississipiensis(American Alligator). Spermiogenesis commences with an acrosome vesicle forming from Golgi transport vesicles. An acrosome granule forms during vesicle contact with the nucleus, and remains posterior until mid to late elongation when it diffuses uniformly throughout the acrosomal lumen. The nucleus has uniform diffuse chromatin with small indices of heterochromatin, and the condensation of DNA is granular. The subacrosome space develops early, enlarges during elongation, and accumulates a thick layer of dark staining granules. Once the acrosome has completed its development, the nucleus of the early elongating spermatid becomes associated with the cell membrane flattening the acrosome vesicle on the apical surface of the nucleus, which aids in the migration of the acrosomal shoulders laterally. One endonuclear canal is present where the perforatorium resides. A prominent longitudinal manchette is associated with the nuclei of late elongating spermatids, and less numerous circular microtubules are observed close to the acrosome complex. The microtubule doublets of the midpiece axoneme are surrounded by a layer of dense staining granular material. The mitochondria of the midpiece abut the proximal centriole resulting in a very short neck region, and possess tubular cristae internally and concentric layers of cristae superficially. A fibrous sheath surrounds only the axoneme of the principal piece. Characters not previously described during spermiogenesis in any other amniote are observed and include (1) an endoplasmic reticulum cap during early acrosome development, (2) a concentric ring of endoplasmic reticulum around the nucleus of early to middle elongating spermatids, (3) a band of endoplasmic reticulum around the acrosome complex of late developing elongate spermatids, and (4) midpiece mitochondria that have both tubular and concentric layers of cristae. J. Morphol., 2010. © 2010 Wiley‐Liss, Inc.
Mineral dust around the Sahara—from source to sink. A review with emphasis on contributions of the German soil science community in the last twenty years
Authors: Herrmann Ludger , Jahn Reinhold , Maurer Thomas 
Published: August 2010

AbstractNatural mineral dust has manifold environmental effects reaching from fertilizing aquatic and terrestrial ecosystems to affecting the earth's radiation balance and thus impacting on climate. The Sahara is considered the largest source of natural mineral dust on the globe, so much research attention has been paid to source identification, dust mobilization, transport, and effects in the sink areas. This paper gives a review of the research results concerning these topics emphasizing soil‐science‐related aspects of the last 20 y and the identification of knowledge gaps.
Hydrogen bonding in different pyrimidine–methanol clusters probed by polarized Raman spectroscopy and DFT calculations
Authors: Singh Dheeraj K. , Mishra Shivangi , Ojha Animesh K. , Srivastava Sunil K. , Schlücker S. , Asthana B. P. , Popp J. , Singh Ranjan K. 
Published: August 2010

AbstractWe report on the hydrogen bonding between pyrimidine (Pd) and methanol (M) as H‐donor in this study. Hydrogen bonds between pyrimidine and methanol molecules as well as those between different methanol molecules significantly influence the spectral features at high dilution. The ring‐breathing mode ν1 of the reference system Pd was chosen as a marker band to probe the degree of hydrogen bonding. Polarized Raman spectra in the region 970–1020 cm−1 for binary mixtures of (pyrimidine + methanol) at 28 different mole fractions were recorded. A Raman line shape analysis of the isotropic Raman line profiles at all concentrations revealed three distinct spectral components at mole fractions of Pd below 0.75. The three components are attributed to three distinct groups of species: ‘free Pd’ (pd), ‘Pd with low methanol content’ (pd1) and ‘Pd with high‐methanol content’ (pd2). The two latter species differ considerably in the pattern and the strengths of the hydrogen bonds. The results of density functional theory calculations on structures and vibrational spectra of neat Pd and eight Pd/M complexes with varying methanol content support our interpretations of the experimental results. A nice spectra–structure correlation for the different cluster subgroups was obtained, similar to earlier results obtained for Pd and water. Apart from N···H and O···H hydrogen bonds between pyrimidine and methanol, O···H hydrogen bonds formed among the methanol molecules in the cluster at high methanol content also play a crucial role in the interpretation of the experimental results. Copyright © 2010 John Wiley & Sons, Ltd.
A method of extracting ginsenosides from Panax ginseng by pulsed electric field
Authors: Hou Jingang , He Shaoyu , Ling Mingsong , Li Wei , Dong Rui , Pan Yongqi , Zheng Yinan 
Published: August 2010

AbstractThe objective of this study was to develop and optimize a pulsed electric field (PEF) extraction method. Various experimental conditions, including electric field intensity and frequency, were evaluated against extraction methods. The content of six major ginsenosides (Rg1, Re, Rb1, Rc, Rb2, and Rd) were quantified by HPLC. The results indicated that the highest yield of the ginsenoside is 12.69 mg/g by PEF using the conditions of 20 kV/cm electric field intensity, 6000 Hz frequency, 70% ethanol–water solution, and 150 L/h velocity. The yield of the ginsenoside of PEF extraction method is higher than the other five methods, such as microwave‐assisted extraction, heat reflux extraction, ultrasonic‐assisted extraction, accelerated solvent extraction, and ultrahigh pressure extraction, The whole extraction process of PEF takes less than 1 s, which is much less than the heat reflux extraction method for 6 h and even newly used technique ultrahigh pressure extraction method of 2 min. The high efficiency, shorter extraction times, and lower energy cost of PEF extraction method can be applied in the industrial production of saponins from Panax ginseng. The PEF extraction method is a promising and constructive method to extract ginsenosides.
Characterization of phenolic and other polar compounds in a lemon verbena extract by capillary electrophoresis‐electrospray ionization‐mass spectrometry
Authors: Quirantes‐Piné Rosa , Arráez‐Román David , Segura‐Carretero Antonio , Fernández‐Gutiérrez Alberto 
Published: August 2010

AbstractIn this study, a CE‐MS method has been developed to carry out the qualitative characterization of a lemon verbena (Lippia citriodora) extract for the first time. The CE and ESI‐MS parameters were optimized with respect to resolution, analysis time and peak shape in order to maximize the number of compounds detected and the sensitivity of their determination. The use of two different MS analyzers, TOF‐MS and IT‐MS, enabled the tentative identification of the major components of this extract. Thus, using this method, 16 compounds were determined. Some of them have been previously identified by HPLC methods, although four compounds were also found for the first time in lemon verbena such as asperuloside, tuberonic acid glucoside or 5′‐hydroxyjasmonic acid 5′‐O‐glucoside, shanzhiside and ixoside. These results demonstrate that CE‐MS generates data complementary to those obtainable by HPLC‐MS and it is particularly suited to the analysis of plant metabolites.
Macroporous molecularly imprinted monolithic polymer columns for protein recognition by liquid chromatography
Authors: Liu Jinxiang , Deng Qiliang , Yang Kaiguang , Zhang Lihua , Liang Zhen , Zhang Yukui 
Published: August 2010

AbstractMacroporous cytochrome c (cyc)‐imprinted monolithic polyarylamide columns were prepared, and applied for the template protein recognition by HPLC. With cyc (18.8 mg) as template, the imprinted monolithic materials were in situ polymerized in an HPLC column tube, with methacrylamide (450 mg), methacrylic acid (15.8 mg), piperazine diacrylamide (720 mg) and ammonium sulfate (390 mg) dissolved in 5 mL of phosphate buffer (pH 7.4), initiated by ammonium persulfate and TEMED. After the reaction, cyc was removed with acetic acid (10%, v/v) containing 10% w/v SDS. The non‐imprinted monolithic column was prepared under the same procedure except without cyc. Retention of cyc and its competitive protein, lysozyme (lys), on molecular‐imprinting polymer (MIP) and non‐imprinted polymer columns was studied. When the pH value of mobile phase was 4.0, on MIP column, the retention factors of cyc and lys were 2.0 and 1.3, respectively. However, those on non‐imprinted polymer column were very similar, both as 1.1. Even in competitive environment, a mixture of cyc and lys could be separated on MIP column under gradient elution, with resolution as 1.2. These results indicate that protein‐imprinted monolithic polymer columns could offer obvious affinity and specific recognition to the template protein.
Corrosion performance of epoxy coatings modified by nanoparticulate SiO2
Authors: Li W. , Tian H. , Hou B. 
Published: August 2010

AbstractElectrochemical impedance spectroscopy (EIS) was employed to investigate the corrosion performance of nanoparticulate SiO2 modified epoxy coatings on carbon steel in 3.5 wt% NaCl solution, coupled with salt spray test. Capacitance‐gravimetric methods and delamination tests were performed to analyze the water uptake behavior and interface stability against delamination of modified coatings, respectively. Four systems were studied, including a clear coating and three pigmented coatings (with 1, 2, and 3 wt% nanoparticulate SiO2). The experimental results showed that nanoparticulate SiO2 particles can improve the anti‐corrosion performance of the coatings and the optimal addition content is 2 wt%. The results obtained with capacitance‐gravimetric measurements showed that the diffusion process of water through epoxy coatings with different pigment volume concentration (PVC) obeyed the second Fick's diffusion law in the initial period. Adding nanoparticulate SiO2 into epoxy coatings can act effectively. The positive influence is attributed to the reaction between nanoparticles and epoxy resin which is confirmed by FTIR, improving the barrier and dispersion effectiveness of coatings. The negative one is increasing the number of pores in the coatings when the adding amount is beyond the critical PVC.
Modular StarPEG‐Heparin Gels with Bifunctional Peptide Linkers
Authors: Tsurkan Mikhail V. , Chwalek Karolina , Levental Kandice R. , Freudenberg Uwe , Werner Carsten 
Published: August 2010

AbstractCell responsive materials are instrumental to regenerative therapies. Here, we report about a novel biohybrid hydrogel that consists of heparin and peptide‐conjugated star‐shaped poly(ethylene glycol), crosslinked by peptide units that combine matrix metalloproteinase (MMP) sensitivity and cell adhesive modules. Taking advantage of the high affinity of vascular endothelial growth factor to heparin, we illustrate the applicability of our hydrogels as a novel system that is supportive of cellular remodeling and three‐dimensional migration of human endothelial cells.magnified image
Resveratrol (3,5,4′‐trihydroxystilbene) protects pregnant mother and fetus from the immunotoxic effects of 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin
Authors: Singh Narendra P. , Singh Ugra S. , Nagarkatti Mitzi , Nagarkatti Prakash S. 
Published: August 2010

AbstractScope: The “fetal basis of adult disease” hypothesis proposes that prenatal exposure to environmental stress can lead to increased susceptibility to clinical disorders later in life.In uteroexposure of fetus to 2,3,7,8‐tetrachlorodibenzo‐p‐dioxin (TCDD) leads to alterations in T‐cell differentiation in the thymus and increased susceptibility to autoimmune disease later in life. TCDD triggers toxicity through activation of aryl hydrocarbon receptor and severely affects maternal and fetal immune system during pregnancy.Methods and results: In this study, using a mouse model, we investigated if administration of resveratrol (RES; 3,5,4′‐trihydroxystilbene) would inhibit immunotoxicity induced by TCDD during pregnancy in the mother and fetus. We observed that RES protected not only normal nonpregnant mice but also pregnant mothers and their fetuses from TCDD‐induced thymic atrophy, apoptosis, and alterations in the expression of T‐cell receptor and costimulatory molecules as well as T‐cell differentiation. In addition, there was significantly reduced expression of CYP1A1 in thymi of both the mother and the fetus when RES was usedin vivopost‐TCDD exposure.Conclusion: In conclusion, these studies demonstrate that consumption of RES, a natural plant product, during pregnancy, may afford protection to the mother and the fetus from the toxicity induced by environmental pollutants that mediate their effects through activation of aryl hydrocarbon receptor.
Olympiade der Tenside
Authors: Hauthal Hermann G. 
Published: August 2010

AbstractNeben der Diskussion wirtschaftlicher Aspekte und von Rohstofffragen zog der 4. Welt‐Tensid‐Kongreß eine positive Bilanz hinsichtlich der umfangreichen Aktivitäten der Branche zum Nachweis der ökologischen Unbedenklichkeit der kommerziell eingesetzten Tenside. Neue Synthesen und Technologies Fortschritte in der Analytik, Charakterisierung und Anwendung von Tensiden waren weitere Schwerpunkte des Programms.