bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of anthocyanin‐rich tea “Sunrouge” on dextran sodium sulfate‐induced colitis in mice
Authors: Akiyama Satoko , Nesumi Atsushi , Maeda‐Yamamoto Mari , Uehara Mariko , Murakami Akira 
Published: March 2012

AbstractSunrouge, an anthocyanin‐rich tea, has similar levels of catechins as “Yabukita,” the most popular green tea cultivar consumed in Japan. Green tea polyphenols (GTPs) have attracted interest due to their potent antioxidative activities combined with a lack of side effects in humans at normal consumption levels. However, we previously reported that high doses (0.5 and 1%) of dietary GTPs can result in deterioration of colitis and failed to prevent colon carcinogenesis in inflamed colons. In the present study, we determined the inhibitory effects of Sunrouge on colitis in dextran sodium sulfate (DSS)‐treated and untreated control mice. Five‐week‐old female ICR mice were administered a single dose of Yabukita or Sunrouge (extracts in 1 mL distilled water) via a stomach tube for 3 weeks. After 1 week of treatment, the mice were divided into four groups (two Yabukita and two Sunrouge groups) and given drinking water with or without 3% DSS for 2 weeks, then they were euthanized. Those treated with DSS developed watery diarrhea and bloody stools, and showed body weight loss, spleen hypertrophy, and shortening of the colon, as well as deteriorations in survival rate, liver function, colon mucosal interleukin‐1β level and expression of phase II detoxification enzyme mRNA. Sunrouge improved these DSS‐induced symptoms, at least in part, whereas Yabukita showed either no effect or adverse effects in regard to some those parameters. It is suggested that the differences between Yabukita and Sunrouge on DSS‐induced colitis might be due to the high levels of anthocyanins found in Sunrouge tea. © 2012 International Union of Biochemistry and Molecular Biology, Inc.
A turning point for Alzheimer's disease?
Authors: de la Torre Jack C. 
Published: March 2012

AbstractDespite an archive of over 73,000 research papers published in the last two decades on the subject of Alzheimer's disease (AD), little clinical progress has been made relative to how people get sporadic AD and what can be done to help them avoid it. This review spotlights strategic steps that could be a turning point in the dramatic lowering of Alzheimer prevalence. The main strategy includes application of four pillars of prevention: 1) early identification of AD vascular risk factors; 2) early detection of AD vascular risk factors; 3) early intervention of AD vascular risk factors based on evidence‐based medical decisions; 4) patient follow‐up to assess and modify interventions as needed. Tandem to these four pillars of prevention, a proactive lifestyle consisting of a healthy diet coupled to physical and mental activity should be applied as part of any therapeutic intervention. We are persuaded by mounting and compelling evidence that AD is a multifactorial disorder kindled by vascular risk factors that generate chronic brain hypoperfusion (CBH) during advanced aging. A pathobiological cascade of biochemical events in the presence of CBH that leads to oxidative stress and neurodegeneration appears to involve multiple biofactors including micronutrients, trace metals, lipids, and pro‐oxidants, as reviewed in this special issue of BioFactors. Modulation of these biofactors may help prevent or control incipient AD. © 2012 International Union of Biochemistry and Molecular Biology, Inc.
Recent advances in applications of capillary electrophoresis with capacitively coupled contactless conductivity detection (CE‐C4D): an update
Authors: Elbashir Abdalla A. , Aboul‐Enein Hassan Y. 
Published: March 2012

ABSTRACTCapillary electrophoresis with a capacitively contactless conductivity detector (CE‐C4D) is becoming a significant useful technique for the analysis of analytes in various fields such as pharmaceutical, biomedical, food and environmental. This review is an update describing the recent developments in the application of CE with a C4D detector. Copyright © 2012 John Wiley & Sons, Ltd.
A rapid and sensitive liquid chromatography–tandem mass spectrometric assay for moexipril, an angiotensin‐converting enzyme inhibitor in human plasma
Authors: Karra Vijaya Kumari , Mullangi Ramesh , Pilli Nageswara Rao , Inamadugu Jaswanth Kumar , Ravi Vasu Babu , Seshagiri Rao J. V. L. N. 
Published: March 2012

ABSTRACTA simple, rapid and sensitive liquid chromatography/tandem mass spectrometry method was developed and validated for the quantification of angiotensin‐converting enzyme inhibitor, moexipril, in human plasma. Benazepril was used as an internal standard (IS). Analyte and IS were extracted from the human plasma by liquid–liquid extraction technique using ethyl acetate. The reconstituted samples were chromatographed on a C18 column by using a mixture of methanol and 0.1% formic acid buffer (85:15, v/v) as the mobile phase at a flow rate of 0.5 mL/min. The calibration curve obtained was linear (r ≥ 0.99) over the concentration range of 0.2–204 ng/mL. The multiple reaction‐monitoring mode was used for quantification of ion transitions at m/z 499.4/234.2 and 425.2/351.1 for moexipril and IS, respectively. The results of the intra‐ and inter‐day precision and accuracy studies were well within the acceptable limits. A run time of 2.0 min for each sample made it possible to analyze more than 400 plasma samples per day. The proposed method was found to be applicable to clinical studies. Copyright © 2012 John Wiley & Sons, Ltd.
Recent Developments in Enediyne Chemistry
Authors: Joshi Mukesh C. , Rawat Diwan S. 
Published: March 2012

AbstractThe enediynes are known for highly potent anticancer, antimicrobial, as well as cytotoxic activities. The discovery of enediynes from natural sources was achieved in late 1980s. They are presently of high interest, because they exert their biological action due to their ability to form a diradical, which abstracts H‐atoms from the DNA backbone, thus causing cell death. Nowadays, the major works are dedicated to the syntheses of enediynes. This review covers recent developments in enediyne chemistry of the last few decades. It is subdivided in six chapters dealing with the discussion of the chemistry and biological significances of enediynes, and the factors responsible for a better activation of enediynes and potent biological evaluations.
New Verticillane Diterpenoids from Cespitularia taeniata
Authors: Chang Jiun‐Yang , Fazary Ahmed Eid , Lin Yu‐Chi , Hwang Tsong‐Long , Shen Ya‐Ching 
Published: March 2012

AbstractChemical investigation of Cespitularia taeniata has led to the isolation of three new verticillanes, cespitulins E–G (1–3, resp.). The structures of these compounds were elucidated by spectroscopic analysis, especially HR‐MS and 2D‐NMR techniques. Compound 1 possesses a rare norverticillane skeleton with two adjacent OH groups at C(5) and C(6), while the seco‐compound 2 with an aldehyde group at C(9) results from an unusual bond cleavage between C(9) and C(10). Pharmacological studies revealed that compound 3 exhibited significant activities on superoxide‐anion generation and elastase release by human neutrophils in response to FMLP/CB. A plausible biogenetic pathway for compound 2 is also discussed.
The Production of Multiple Small Peptaibol Families by Single 14‐Module Peptide Synthetases in Trichoderma/Hypocrea
Authors: Degenkolb Thomas , Karimi Aghcheh Razieh , Dieckmann Ralf , Neuhof Torsten , Baker Scott E. , Druzhinina Irina S. , Kubicek Christian P. , Brückner Hans , von Döhren Hans 
Published: March 2012

AbstractThe most common sequences of peptaibiotics are 11‐residue peptaibols found widely distributed in the genus Trichoderma/Hypocrea. Frequently associated are 14‐residue peptaibols sharing partial sequence identity. Genome sequencing projects of three Trichoderma strains of the major clades reveal the presence of up to three types of nonribosomal peptide synthetases with 7, 14, or 18–20 amino acid‐adding modules. Here, we provide evidence that the 14‐module NRPS type found in T. virens, T. reesei (teleomorph Hypocrea jecorina), and T. atroviride produces both 11‐ and 14‐residue peptaibols based on the disruption of the respective NRPS gene of T. reesei, and bioinformatic analysis of their amino acid‐activating domains and modules. The sequences of these peptides may be predicted from the gene sequences and have been confirmed by analysis of families of 11‐ and 14‐residue peptaibols from the strain 618, termed hypojecorins A (23 sequences determined, 4 new) and B (3 sequences determined, 2 new), and the recently established trichovirins A from T. virens. The distribution of 11‐ and 14‐residue products is strain‐specific and depends on growth conditions as well. Possible mechanisms of module skipping are discussed.
Synthesis and in vitro Inhibition Properties of siRNA Conjugates Carrying Acridine and Quindoline Moieties
Authors: Aviñó Anna , Ocampo Sandra M. , Perales José C. , Eritja Ramon 
Published: March 2012

AbstractThe synthesis of RNA molecules carrying acridine or quindoline residues at their 3′‐ and 5′‐termini is reported. These conjugates are fully characterized by MALDI‐TOF mass spectrometry. Modified siRNA duplexes carrying acridine or quindoline moieties were evaluated for inhibition of the tumor necrosis factor. The conjugates showed inhibitory properties similar to those of unmodified RNA duplexes in HeLa cells transfected with oligofectamine. The fluorescent properties of acridine derivatives allow direct observation of the cytoplasmatic distribution of modified siRNA inside the cells.
Transcatheter embolization of anomalous systemic arterial supply with amplatzer vascular plugs II in scimitar syndrome
Authors: Noble Stéphane , Miró Joaquim , Ibrahim Réda 
Published: March 2012

AbstractA 57‐year‐old woman suffering from recurrent pneumonia and considered to be high risk for surgical correction of an atrial septal defect (ASD) associated with a right lung sequestration vascularized by arteries coming from the abdominal aorta and an abnormal venous drainage into the inferior vena cava (Scimitar syndrome). Therefore stepwise transcatheter approach was offered as an alternative treatment. We performed ASD closure and embolization of the anomalous systemic arterial supply using Amplatzer Vascular Plugs II. The abnormal venous drainage was conservatively treated (Qp/Qs: 1.1/1 after ASD closure and anomalous arterial supply embolization). At 24‐month follow‐up, no recurrent pneumonia was reported, functional class improved and right cardiac cavities normalized. © 2012 Wiley Periodicals, Inc.
Percutaneous closure of an atrial septal defect in an infant with shone's syndrome
Authors: Petit Christopher J. , Justino Henri , Fraser Charles D. 
Published: March 2012

AbstractRarely is closure of the atrial septal defect indicated in infants. Yet, an atrial septal defect in the presence of congenital mitral stenosis may cause significant congestive heart failure in infancy. We present the case of an infant with Shone's syndrome, left ventricular hypoplasia, and a large ostium secundum atrial septal defect who developed pulmonary overcirculation following repair of coarctation of the aorta. The infant underwent cardiac catheterization, hemodynamic assessment, and successful percutaneous closure of the atrial septal defect. The patient improved dramatically following the intervention. Follow‐up echocardiograms have demonstrated growth of mitral valve annulus diameter and left ventricular dimensions. Atrial septal defect closure in this small infant was associated with immediate improved clinical status, and improved growth of the left heart structures. © 2012 Wiley Periodicals, Inc.
Procedural and clinical outcomes of the valve‐in‐valve technique for severe aortic insufficiency after balloon‐expandable transcatheter aortic valve replacement
Authors: Giri Jay , Bortnick Anna E. , Wallen Tyler , Walsh Elizabeth , Bannan Amr , Desai Nimesh , Szeto Wilson Y. , Bavaria Joseph , Herrmann Howard C. 
Published: March 2012

AbstractObjective: To describe the clinical and procedural outcomes of patients treated with the valve‐in‐valve technique for severe aortic insufficiency (AI) after balloon‐expandable transcatheter aortic valve replacement (TAVR). Background: Severe AI immediately after valve implantation is a notable complication of TAVR. It can be treated with a valve‐in‐valve technique which involves deploying a second valve within the first one to crush the leaflets of the first implant leaving a new functional valve. Methods: We analyzed data on 142 consecutive patients at our institution undergoing TAVR with the Sapien valve between November of 2007 and April of 2011. Etiologies of acute AI, procedural and intermediate term clinical outcomes were reported for those in whom a valve‐in‐valve procedure was necessary. Post‐hoc analysis of these cases with C‐THV imaging (Paieon Medical Ltd.) was performed to elucidate the mechanism for successful AI treatment. Results: A total of 5 of 142 (3.5%) patients were treated with the valve‐in‐valve technique. Etiologies of the aortic valve insufficiency included bioprosthesis malposition (n = 3), valve dysfunction (n = 1), and valve undersizing (n = 1). With placement of the second valve, the first valve dimensions increased to approach the nominal valve size while the second valve size remained less than nominal. Conclusions: The valve‐in‐valve technique is an appropriate bailout measure for patients with acute valvular AI after balloon‐expandable TAVR. © 2012 Wiley Periodicals Inc.
Upper cavo‐pulmonary anastomosis by transcatheter technique
Authors: Schmitt Boris , Sabi Titus Mbah , Sigler Matthias , Berger Felix , Ewert Peter 
Published: March 2012

AbstractObjectives: The aim of this animal study was to establish a shunt connection between superior vena cava (SVC) and right pulmonary artery (RPA) by transvascular intervention solely. Background: After initial shunt creation, the establishment of the upper cavo‐pulmonary anastomosis (UCPA) is the second out of three open chest operations young infants with univentricular anatomy are subjected to. To avoid the risks of reoperation with cardiovascular bypass, we sought to replace this surgical step by an interventional technique. Methods: After cannulation of jugular and femoral veins in four piglets (mean body weight of 12.5 kg) an UCPA was created by radiofrequency perforation from the SVC across the right atrium into the RPA and subsequent implantation of covered stents. The perforation was guided by biplane fluoroscopy and the perforation wire premounted with a coaxial catheter was advanced into the distal pulmonary artery and exchanged for a stiffer wire. A long sheath was brought into the RPA and an 80 mm long expanded poly‐tetra‐fluoro‐ethylene (ePTFE)‐covered Cheatham Platinum stent was then implanted connecting the SVC with the RPA. Results: Immediate angiography showed antegrade flow from SVC to RPA. Angiographic re‐evaluation after a median period of 4 weeks showed partial in‐stent stenosis but patent lumina. Additionally, veno‐venous collaterals from the SVC to the right atrium had developed. Histology of the explanted stents revealed parietal thrombi and mild to moderate pseudo intima proliferation inside the lumina. Conclusions: The transvascular creation of an upper unidirectional cavo‐pulmonary anastomosis in piglets is technically feasible using standard catheterization equipment. © 2012 Wiley Periodicals, Inc.
Clinical outcomes with contemporary endovascular therapy of iliac artery occlusive disease
Authors: Dattilo Philip B. , Tsai Thomas T. , Garcia Joel A. , Allshouse Amanda , Casserly Ivan P. 
Published: March 2012

AbstractObjectivesWe sought to evaluate the clinical outcomes of a consecutive series of patients treated for iliac artery occlusive disease (IAOD) using contemporary endovascular technology and techniques.BackgroundAs an increasingly complex spectrum of IAOD is treated using endovascular revascularization, there is a need to examine the rates of acute procedural success, complications, and patency to validate the role of an endovascular‐first approach to revascularization in contemporary practice.MethodsAll patients with IAOD who were treated using endovascular therapy between September 2005 and September 2010 were identified from a prospectively collected database. Baseline patient characteristics, anatomic details, procedural data, and clinical outcomes were assessed retrospectively. Patency and mortality rates were estimated with the Kaplan–Meier method.ResultsA total 59 patients underwent 62 procedures. Trans‐Atlantic Inter‐Society Consensus (TASC) II types B, C, and D disease accounted for 59%, 7%, and 37% of patients, respectively. The procedure was technically successful in 60 of 62 cases (97%) with no procedure‐related mortality. Major complications occurred in five procedures (8%). The mean (±standard deviation) duration of follow‐up was 2.3 ± 1.4 years. In patients with a successful revascularization, primary and secondary patency rates were 86% and 94% at 1 year, and 77% and 91% at 2 years, respectively. The TASC II classification of disease did not predict the rate of acute technical success or medium‐term patency rates.ConclusionsThe acute and medium‐term clinical outcomes of this series of patients with anatomically complex IAOD support the current paradigm of an endovascular‐first approach to revascularization. © 2012 Wiley Periodicals Inc.
In‐Hospital and 1‐year outcomes with drug‐eluting versus bare metal stents in saphenous vein graft intervention: A report from the EVENT registry
Authors: Tolerico Paul H. , Cohen David J. , Kleiman Neal S. , Berger Peter B. , Brilakis Emmanouil S. , Piana Robert N. , Shammo Salim , Keyes Michelle J. , Kennedy Kevin F. , Massaro Joseph M. , Saucedo Jorge F. ,   on behalf of the EVENT Investigators
Published: March 2012

AbstractObjectivesTo compare outcomes of patients receiving drug‐eluting stents (DES) versus bare metal stents (BMS) during percutaneous coronary intervention (PCI) of saphenous vein bypass grafts (SVG).BackgroundLong‐term benefits of DES versus BMS are well established for native vessel PCI. Benefit in patients undergoing SVG intervention is less certain. We used data from a multicenter registry (evaluation of drug eluting stents and ischemic events, EVENT) to compare outcomes among patients treated with DES versus BMS 1‐year following SVG interventions.MethodsBetween July 2004 and December 2007, 684 patients in EVENT underwent SVG PCI (515 DES only, 169 BMS only). The primary endpoint was a composite of death, myocardial infarction (MI), and target lesion revascularization between hospital discharge and 1‐year follow‐up. Propensity score stratification was used to adjust for differences between groups.ResultsBaseline demographic and clinical characteristics of patients treated with DES and BMS were similar. The DES group had fewer men and a higher prevalence of prior PCI. Patients receiving DES had less angiographic thrombus, less frequent use of embolic protection devices, greater total stent length, and smaller maximum stent diameters. Unadjusted outcomes between discharge and 1‐year follow‐up did not differ between the groups. After risk adjustment, the primary outcome was less frequent among patients treated with DES (adjusted HR = 0.48, 95% CI = 0.27–0.84, P < 0.01) with similar relative benefits across the individual endpoints.ConclusionsAmong patients undergoing SVG PCI in a “real world” registry analyzed using propensity score stratification, treatment with DES compared with BMS was associated with reduced MACE at 1 year following PCI. © 2012 Wiley Periodicals, Inc.
Coil‐occlusion of the left ventricle as emergency treatment in failing stage I palliation for hypoplastic left heart syndrome with sinusoids
Authors: Hummel Johanna , Stiller Brigitte , Grohmann Jochen 
Published: March 2012

AbstractWe report on a patient with hypoplastic left heart syndrome (HLHS), ventricular septal defects, and coronary sinusoids who suffered recurrent myocardial ischemic events that required cardiopulmonary resuscitation after stage I palliation. We identified the main reason to be a steal phenomenon of blood from the left coronary artery via the sinusoids into the rudimentary left ventricle and across the septal defects into the right ventricle. To limit this coronary steal phenomenon, we successfully performed transcatheter closure of the left ventricle with implantation of three Microplex Terumo®‐Coils. This led to the patient's clinical stabilization. Stage II surgery took place at the age of 5 months without difficulty. In the setting of a symptomatic neonate with HLHS and ventriculocoronary connections, embolization of the left ventricle is a feasible interventional therapy. © 2012 Wiley Periodicals, Inc.
Preclosure of large‐sized venous access sites in adults undergoing transcatheter structural interventions
Authors: Hamid Tahir , Rajagopal Rajinikanth , Pius Charlene , Clarke Bernard , Mahadevan Vaikom S. 
Published: March 2012

BackgroundPatients requiring congenital and structural heart interventions often require large‐sized sheath insertion into femoral veins and arteries. Clinical outcome data on the use of suture‐mediated devices for femoral venous access site closure are limited.ObjectiveTo assess the efficacy and safety of the Perclose™ (Abbott Vascular Devices, CA) suture‐mediated device using the preclosure technique, in achieving haemostasis at femoral venous access site following large sheath insertion (≥8 Fr).Design and SettingTwo hundred and forty‐three consecutive patients underwent 310 access site closures with the Perclose™ device using the preclosure technique. There were 151 (62%) women, mean age 43 (±16) years. 234/243 (96%) received heparin. Mean venous access site sheath diameter was 11.5 (±3) Fr.ResultsImmediate haemostasis (<2 min) was achieved in 304/310 (98%) sites. No patients had major complications. Short‐term follow‐up at 3 months revealed no evidence of haematoma or fistula formation or clinical evidence of vessel occlusion. On medium‐term clinical follow‐up (mean follow‐up of 14 ± 12 and median of 12.4 months), no complications were seen at the venous access sites.ConclusionPreclosure of large‐size femoral venous access sheath sites using the suture‐mediated Perclose™ device is efficacious in achieving rapid haemostasis in the presence of anticoagulation in the venous site. On 1‐year follow‐up, there was no clinical evidence of vascular complications in the venous access sites. © 2012 Wiley Periodicals, Inc.