bluuebunny/crossref_metadata_2025_split huggingface dataset:
Peri‐implant soft tissue and bone crest alterations at fixed dental prostheses: a 3‐year prospective study
Authors: Chang Moontaek , Wennström Jan L. 
Published: March 2010

AbstractObjectives: The aim of this 3‐year prospective study involving patients receiving implant‐supported fixed dental prostheses (FDPs) was to longitudinally evaluate soft and hard tissue alterations from the time of implant placement.Material and methods: Sixteen subjects with 18 implant‐supported FDPs on 43 implants were included in the study. Peri‐implant soft and hard tissue assessments were performed at implant placement, 2, 6, 12, 24 and 36 months. Variables describing the peri‐implant soft tissue conditions and topography were assessed by clinical, photographic and study model assessments. Variables of implant position and bone topography were measured in radiographs taken at each examination interval. Multilevel regression models were formulated to identify factors of significance for proximal bone crest alterations and proximal soft tissue height.Results: During the first 6 months after the one‐stage implant placement surgery, the soft tissue margin receded about 0.6 mm at facial implant sites, while a mean increase was observed at tooth‐facing proximal sites (1.1 mm) and no change at inter‐implant sites. Loss in proximal bone crest height was more pronounced at inter‐implant than tooth–implant units at 6 months (0.6 vs. 0.1 mm). Between 6 and 36 months, no further significant soft or hard tissue changes were observed. Multilevel regression analysis revealed that the proximal bone crest level significantly influenced the proximal soft tissue height assessed from the implant/abutment level. Significant predictors for loss in proximal bone crest level over the 3 years were horizontal inter‐unit distance, type of proximal unit (tooth/implant or inter‐implant) and peri‐implant bone‐level change.Conclusions: Soft and hard tissue changes around implant‐supported FDPs took place primarily during the first 6 months after the one‐stage implant installation surgery. The pattern of tissue alterations during the follow‐up differed between tooth–implant and inter‐implant proximal sites. To cite this article:
Chang M, Wennström JL. Peri‐implant soft tissue and bone crest alterations at fixed dental prostheses: a 3‐year prospective study. Clin. Oral Impl. Res. 21, 2010; 527–534. doi: 10.1111/j.1600‐0501.2009.01874.x
Factors influencing resonance frequency analysis assessed by Osstell™mentor during implant tissue integration: I. Instrument positioning, bone structure, implant length
Authors: Sim Christina P. C. , Lang Niklaus P. 
Published: March 2010

AbstractAim: To monitor longitudinally the development of implant stability of SLA Straumann® tissue‐level implants using resonance frequency analysis (RFA) and to determine the influence of instrument positioning, bone structure and implant length on the assessment of RFA.Material and methods: Thirty‐two healthy adult patients received either 8 mm, ∅4.1 mm Straumann® Standard Plus tissue‐level implants (n=16: Group A) or 10 mm, ∅4.1 mm Straumann® Standard Plus tissue‐level implants (n=16: Group B). During healing, RFA was performed on Weeks 0,1, 2, 3, 4, 5, 6, 8 and 12. The implants were restored after 10 weeks (impression taking) and 12 weeks. In addition, probing depth, presence of plaque and bleeding on probing were assessed. Implant stability quotient (ISQ) values of Groups A and B were compared using unpaired t‐tests and longitudinally applying paired t‐tests between Week 0 and the subsequent time points.Results: Positioning of the Osstell™mentor device did not affect the ISQ values. Generally, ISQ values increased continuously during healing from a mean of 65.1 (SD 16.97) to 74.7 (SD 5.17) (significantly from Week 0 to Weeks 6, 8 and 12). Lower bone density (Type III or IV) resulted in significantly lower ISQ values up to Week 8. Implant length affected the increase in ISQ values over time. While no significant increase was observed with 10 mm implants, ISQ values of 8 mm implants increased significantly from Week 0 to Weeks 6, 8 and 12.Conclusions: Using Osstell™mentor, ISQ values are reproducible irrespective of instrument positioning. ISQ values are affected by the bone structure and implant length. Hence, no predictive values can be attributed to implant stability. To cite this article:
Sim CPC, Lang NP. Factors influencing resonance frequency analysis assessed by Osstell™mentor during implant tissue integration: I. Instrument positioning, bone structure, implant length. Clin. Oral Impl. Res. 21, 2010; 598–604. doi: 10.1111/j.1600‐0501.2009.01878.x
Cement thickness at implant‐supported single‐tooth Lava assemblies: a scanning electron microscopic investigation
Authors: Apicella D. , Veltri M. , Chieffi N. , Balleri P. , Ferrari M. 
Published: March 2010

AbstractObjectives: The fit of implant‐supported single‐tooth Lava zirconia assemblies was investigated in this study. The implant–abutment interface, the interface between the metallic and the zirconia portion of the abutment and the interface between Lava abutments and copings were evaluated. The adaptation of titanium abutments to implants and Lava copings was investigated as a control.Material and methods: Twenty implants were randomly assigned and connected to Lava abutments (group 1) or titanium abutments (group 2). All specimens were subjected to scanning electron microscopy (SEM) analysis of the fixture/abutment fit. Afterwards, specimens were luted to Lava copings and subjected to a SEM evaluation of the marginal external adaptation of the abutments with the copings. Finally, the samples were embedded in resin, sectioned and subjected to SEM analysis of the following interfaces; group 1: titanium/zirconia interface (between the constitutive components of the Lava abutment) and the zirconia/zirconia interface (between the Lava abutment and the coping); group 2: the titanium/zirconia interface (between the titanium abutment and the Lava coping). Non‐parametric analysis of variance and a post hoc test were used for statistical analysis.Results: Significant differences emerged in the cement thickness between titanium and zirconia components of the Lava abutments as compared with the thickness measured at the interface between Lava copings and the abutments investigated. No differences were found in cement thickness between Lava copings and the two different abutments.Conclusions: When Lava abutments are used, the most critical cement thickness is the internal interface between its titanium and zirconia components. Lava coping adaptation for both Lava and titanium abutments is within the clinical acceptable range. To cite this article:
Apicella D, Veltri M, Chieffi N, Balleri P, Ferrari M. Cement thickness at implant‐supported single‐tooth Lava assemblies: a scanning electron microscopic investigation. Clin. Oral Impl. Res. 21, 2010; 747–750. doi: 10.1111/j.1600‐0501.2009.01882.x
Long‐term response of osteogenic cells on micron and submicron‐scale‐structured hydrophilic titanium surfaces: sequence of cell proliferation and cell differentiation
Authors: Klein Marcus Oliver , Bijelic Ana , Toyoshima Takeshi , Götz Hermann , Von Koppenfels Roxana Laura , Al‐Nawas Bilal , Duschner Heinz 
Published: March 2010

AbstractObjective: Modifications of surface topography and surface chemistry are key factors for guiding target cells during dental implant healing. Recent in vitro studies confirmed promotion of early osteogenic cell differentiation on submicron scaled surfaces in particular when hydrophilized. However, no long‐term observations on both osteogenic cell proliferation as well as on cell maturation have been reported for respectively modified surfaces. Aim of this study was to monitor osteogenic cell proliferation and expression of specific osteogenic cell differentiation markers on a protein level over an extended period of 3 weeks with respect to surface modifications.Material and methods: Modified titanium (Ti) disks were obtained from Institute Straumann, representing the following surfaces: smooth pretreatment (PT), sandblasted/acid etched (SLA), and hydrophilized (modSLA). Surface topography was analyzed by scanning electron microscopy, surface elemental composition was assessed by X‐Ray Photoelectronic Spectroscopy (XPS). Tissue culture polystyrene (TCPS) served as a control substrate. Human osteogenic cells (HOB‐c) were cultivated on the respective substrates. After 24 hrs, 48 hrs, 72 hrs, 7 d, 14 d and 21 d, cell count was assessed as well as osteogenic cell differentiation utilizing cellular Quantitative Immuno‐Cytochemistry (QIC) assay for collagen type I (COL), alkaline phosphatase (AP), osteopontin (OPN) and osteocalcin (OC). Data were normalized with respect to internal controls.Results: In contrast to the other modified Ti disks, modSLA stands out due to low surface carbon contamination. TCPS and PT surfaces preserved a rather immature, mitotic active osteogenic phenotype (high proliferation rates, no increase of OC production), SLA and especially modSLA surfaces promoted the maturation of osteogenic precursors into post‐mitotic osteoblasts. In detail, modSLA resulted in lowest cell proliferation rates, but exhibited highest expression rates of OC.Discussion: Our results, which confirm previous studies, reveal long‐term promotion of osteogenic cell maturation by topography (micron and submicron scale roughness) and surface hydrophilicity. To cite this article:
Klein MO, Bijelic A, Toyoshima T, Götz H, von Koppenfels RL, Al‐Nawas B, Duschner H. Long‐term response of osteogenic cells on micron and submicron‐scale‐structured hydrophilic titanium surfaces: sequence of cell proliferation and cell differentiation.  Clin. Oral Impl. Res. 21, 2010; 642–649. doi: 10.1111/j.1600‐0501.2009.01883.x
Biological width following immediate implant placement in the dog: flap vs. flapless surgery
Authors: Blanco Juan , Alves Célia Coutinho , Nuñez Vanesa , Aracil Luis , Muñoz Fernando , Ramos Isabel 
Published: March 2010

AbstractObjective: To assess the marginal soft tissue healing process after flap or flapless surgery in immediate implant placement in a dog model.Material and methods: This study was carried out on five Beagle dogs. Four implants were placed in the lower jaw in each dog immediately after tooth extraction. Flap surgery was performed before the extraction on one side (control) and flapless on the other (test). After 3 months of healing, the dogs were sacrificed and prepared for histological analysis.Results: Ten implants were placed in each group. Two failed (one of each group). The length of the junctional epithelium in the flapless group was 2.54 mm (buccal) and 2.11 mm (lingual). In the flap group, the results were very similar: 2.59 mm (buccal) and 2.07 mm (lingual), with no significant differences observed between the groups. The length of the connective tissue in the flapless group was 0.68 mm (buccal) and 0.54 mm (lingual), and 1.09 mm at the buccal and 0.91 mm at the lingual aspect in the flap group, with no significant differences between groups. The difference between the mean distance from the peri‐implant mucosa margin to the first bone–implant contact at the buccal aspect was significant between both groups (3.02 mm‐flapless and 3.69 mm flap group). However, this difference was mostly due to the Pm3 group (flapless: 2.95/flap: 3.76) because no difference could be detected in the Pm4 group. Both groups showed minimal recession, with no significant differences between groups (flapless group – 0.6 mm buccal and 0.42 mm lingual; flap group – 0.67 and 0.13 mm).Conclusion: The clinical evaluation of immediate implant placement after 3 months of healing indicated that buccal soft tissue retraction was lower in the flapless group than in the flap group, without significant differences. The mean values of the biological width longitudinal dimension at the buccal aspect were higher in the flap group than in the flapless group, this difference being mostly due to the Pm3, probably because of a thinner biotype in this region. To cite this article:
Blanco J, Alves CC, Nuñez V, Aracil L, Muñoz F, Ramos I. Biological width following immediate implant placement in the dog: flap vs. flapless surgery. Clin. Oral Impl. Res. 21, 2010; 624–631. doi: 10.1111/j.1600‐0501.2009.01885.x
Multiple myeloma: chemotherapy or transplantation in the era of new drugs
Authors: Palumbo Antonio , Rajkumar S. Vincent 
Published: March 2010

Abstract Objective: To review the current results of studies incorporating novel agents in multiple myeloma (MM) and discuss the role of autologous stem‐cell transplantation (ASCT) in the era of new active drugs for the treatment of this disease. The outlook for patients with symptomatic MM is changing with the introduction of bortezomib, thalidomide, and lenalidomide into the repertoire of available chemotherapeutic agents. Compared with standard chemotherapy, a survival benefit has been reported for the first time in 30 yrs. Methods: Articles published in English between 1969 and 2008 were identified by searching PubMed for ‘myeloma’, ‘diagnosis’, ‘thalidomide’, ‘bortezomib’, ‘lenalidomide’, ‘dexamethasone’, ‘prednisone’, ‘doxorubicin’, ‘cyclophosphamide’, ‘melphalan’, ‘combination chemotherapy’, and ‘autologous transplantation’. Results: In randomized studies, bortezomib, thalidomide, and lenalidomide have each been combined with dexamethasone, alkylating agents, or doxorubicin, and such combinations resulted in significant improvement in progression‐free survival. Conclusions: The incorporation of new drugs as induction therapy along with ASCT appears to produce very good partial response rates, slightly superior to those achieved by conventional chemotherapy with new drugs. How to best optimize induction, consolidation, and maintenance therapy and how to best select and prepare patients for ASCT are still to be determined. Randomized trials are needed to directly compare the current best chemotherapeutic approach with best ASCT strategies and to guide clinical practice for patients with MM.
Cancer‐associated microangiopathic hemolytic anemia with thrombocytopenia: an important diagnostic consideration
Authors: Elliott Michelle A. , Letendre Louis , Gastineau Dennis A. , Winters Jeffrey L. , Pruthi Rajiv K. , Heit John A. 
Published: March 2010

AbstractBackground:  Early initiation of plasma exchange (PE) allows more than 80% of patients with idiopathic thrombotic thrombocytopenic purpura (TTP), most commonly because of severe ADAMTS13 deficiency, to achieve remission and mandates urgency in diagnosis and therapy. Metastatic cancer may present with a microangiopathic hemolytic anemia with thrombocytopenia that is clinically similar to TTP but does not respond to PE. ADAMTS13 activity can be diagnostic but usually not immediately available. Recognition of cancer‐associated microangiopathic hemolytic anemia with thrombocytopenia (CA‐MHA) is paramount to avoid inappropriate PE therapy and delays in cancer‐specific chemotherapy.Objective:  To identify distinguishing characteristics of CA‐MHA and TTP to facilitate early recognition of CA‐MHA.Methods:  In a retrospective cohort study, baseline clinical and laboratory data of consecutive adult patients with CA‐MHA (n = 7) or autoimmune TTP (n = 7) from a registry of patients with clinically suspected acute TTP referred for PE were compared.Results:  The frequencies of bone pain and respiratory symptoms were significantly greater among patients with CA‐MHA compared to patients with TTP; other baseline clinical and laboratory characteristics did not differ significantly between the two groups. Response to PE and mortality at day 30 were significantly worse for CA‐MHA (14% and 71%, respectively) compared to patients with TTP (86% and 14%, respectively).Conclusions:  Baseline clinical and laboratory characteristics largely do not distinguish acute CA‐MHA from autoimmune acute TTP. While all suspected acute patients TTP should receive urgent PE, bone pain, respiratory symptoms, or inadequate PE response should prompt an early search for CA‐MHA.
REVIEW ARTICLE: Immunology of Pre‐Eclampsia
Authors: Redman Christopher W. G. , Sargent Ian L. 
Published: March 2010

Citation Redman CWG, Sargent IL. Immunology of Pre‐eclampsia. Am J Reprod Immunol 2010Pre‐eclampsia develops in stages, only the last being the clinical illness. This is generated by a non‐specific, systemic (vascular), inflammatory response, secondary to placental oxidative stress and not by reactivity to fetal alloantigens. However, maternal adaptation to fetal (paternal alloantigens) is crucial in the earlier stages. A pre‐conceptual phase involves maternal tolerization to paternal antigens by seminal plasma. After conception, regulatory T cells, interacting with indoleamine 2,3‐dioxygenase, together with decidual NK cell recognition of fetal HLA‐C on extravillous trophoblast may facilitate placental growth by immunoregulation. Complete failure of this mechanism would cause miscarriage, while partial failure would cause poor placentation and dysfunctional uteroplacental perfusion. The first pregnancy preponderance and partner specificity of pre‐eclampsia can be explained by this model. For the first time, the pathogenesis of pre‐eclampsia can be related to defined immune mechanisms that are appropriate to the fetomaternal frontier. Now, the challenge is to prove the detail.
CO2 laser‐assisted treatment of a giant pyogenic granuloma of the gingiva
Authors: Lindenmüller I Hitz , Noll P , Mameghani T , Walter C 
Published: March 2010

To cite this article:

Int J Dent Hygiene 8, 2010; 249–252 DOI: 10.1111/j.1601‐5037.2010.00449.x Lindenmüller IH, Noll P, Mameghani T, Walter C. CO2 laser‐assisted treatment of a giant pyogenic granuloma of the gingiva.Abstract:  Background:  Pyogenic granuloma (PG) may develop in the oral cavity of pregnant women. The periodontal treatment outcomes during and after pregnancy are described.Methods:  A 34‐year‐old woman in the 39th week of pregnancy presented for surgical treatment with a mass on the lingual mandibular gingiva. She had been surgically treated alio loco in the 37th week, but this failed. The patient was reassured and an individual oral hygiene programme was initiated in our department. The tumour was about 20 mm in diameter. A CO2 laser‐assisted surgical excision was performed 4 weeks after delivery. The lesion was analysed histopathologically using a von Willebrand Factor immunoreactivity staining. The highly vascularized tissue with a dense inflammatory infiltrate was in accordance with the diagnosis of a PG.Results:  The initial wound healing was uneventful. A 12‐month follow‐up revealed no recurrence of the mass and healthy periodontal tissues.Conclusion:  This report describes an oral complication during pregnancy for which surgical excision of a PG after delivery seemed the best treatment. It is possible that gender‐specific periodontal disease risk factors contributed to the development of the lesion. This is another reason why pregnant women should be encouraged to be assessed by oral health professionals before late pregnancy.
The erosive potential of jawbreakers, a type of hard candy
Authors: Brand HS , Gambon DL , Van Dop LF , Van Liere LE , Veerman ECI 
Published: March 2010

To cite this article:

Int J Dent Hygiene 8, 2010; 308–312 DOI: 10.1111/j.1601‐5037.2010.00450.x Brand HS, Gambon DL, van Dop LF, van Liere LE, Veerman ECI. The erosive potential of jawbreakers, a type of hard candy.Abstract:  Objectives:  To explore the consumption pattern of a specific type of acidic solid candy, the so‐called jawbreakers, by primary school children and determine the erosive potential of this type of candy in vivo.Methods:  A questionnaire about jawbreaker consumption was distributed among 10–12 year‐old‐children (n = 302). Subsequently, 19 healthy volunteers tested four different jawbreakers in vivo. Whole saliva was collected 5 min before, 3 min during and 11 min after consumption. Salivary flow rate and pH were determined.Results:  Two‐thirds of the children reported a history of jawbreaker consumption, 18% during the last week. More than half of the children estimated their average time for consumption of one jawbreaker to be more than 15 min. In vivo, the jawbreakers induced 8.6–13.9‐fold increase in salivary flow rate. Sucking on sour, jumbo and strawberry jawbreakers induced a drop in salivary pH to values below pH 5.5. During consumption of fireball jawbreakers, the intra‐oral pH hardly changed.Conclusions:  Jawbreakers are frequently used by children, who keep this candy in their mouth for a long time. Jawbreakers differ considerable in erosive potential, with sour and jumbo jawbreakers > strawberry jawbreaker >> fireball jawbreaker. This information is of use for dental hygienists counselling juvenile patients with dental erosion.
Tetracysteine‐tagged prion protein allows discrimination between the native and converted forms
Authors: Gašperšič Jernej , Hafner‐Bratkovič Iva , Stephan Michel , Veranič Peter , Benčina Mojca , Vorberg Ina , Jerala Roman 
Published: March 2010

The conformational conversion of prion protein (PrP) from a native conformation to the amyloid form is a hallmark of transmissible spongiform encephalopathies. Conversion is usually monitored by fluorescent dyes, which bind generic amyloids and are less suited for living cell imaging. We report a new method for the synthesis of membrane‐permeable and membrane‐impermeable biarsenical reagents, which are then used to monitor murine PrP (mPrP) misfolding. We introduced tetracysteine (TC) tags into three different positions of mPrP, which folded into a native‐like structure. Whereas mPrPs with a TC tag inserted at the N‐terminus or C‐terminus supported fibril formation, insertion into the helix 2–helix 3 loop inhibited conversion. We devised a quantitative protease‐free method to determine the fraction of converted PrP, based on the ability of the fluorescein arsenical helix binder reagent to differentiate between the monomeric and fibrilized form of TC‐tagged PrP, and showed that TC‐tagged mPrP could be detected on transfected cells, thereby expanding the potential use of this method for the detection and study of conformational diseases.Structured digital abstract
MINT‐7709757: Prp (uniprotkb:P04925) and Prp (uniprotkb:P04925) bind (MI:0407) by electron microscopy (MI:0040)
MINT‐7709744: Prp (uniprotkb:P04925) and Prp (uniprotkb:P04925) bind (MI:0407) by circular dichroism (MI:0016)
MINT‐7709730: Prp (uniprotkb:P04925) and Prp (uniprotkb:P04925) bind (MI:0407) by fluorescence technology (MI:0051)
EVALUATION OF THE PETRIFILM DRY REHYDRATABLE FILM FOR THE ENUMERATION OF YEASTS AND MOULDS IN NATURALLY CONTAMINATED FOODS
Authors: TOURNAS VALERIE H. , FELICIANO LIZANEL , KATSOUDAS EUGENIA J. 
Published: March 2010

ABSTRACT Two hundred thirty‐seven samples from six food groups (tree nuts, grains and grain products, dried fruits, fresh produce, fruit juice and dairy products) were tested for levels of fungal contamination using the Petrifilm dry rehydratable film for yeast and mould (PYM ) enumeration (3M Microbiology, St. Paul, MN) and the Food and Drug Administration (FDA) official method. Results indicated that PYM performed very well for all tested commodities giving YM counts similar to those of the FDA method. Statistical analysis of the data (t‐test) revealed no significant differences between the two methods for all foods tested. Linear regression analysis showed that the correlation coefficients between the two methods were over 0.90 for 91% of the commodities tested. Some difficulties were encountered during counting of the colonies on PYM as yeast colonies on this medium tend to be very small with rather faded colorations.PRACTICAL APPLICATIONSAssessment of the efficacy of Petrifilm and determining its equivalence to traditional, validated methods will give the testing laboratories and the industry an alternate option for the enumeration of fungi in foods. The use of Petrifilm would be especially useful when analyst time is limited as it requires no media preparation and minimal time for sample inoculation.