bluuebunny/crossref_metadata_2025_split huggingface dataset:
CAV1 promotes epithelial-to-mesenchymal transition (EMT) and chronic renal allograft interstitial fibrosis by activating the ferroptosis pathway
Authors: Han Qianguang , Ni Bin , Bao Wei , Zhang Junqi , Zheng Ming , Miu Jinxu , Wang Zijie , Yuan Jingwen , Tao Jun , Han Zhijian , Gu Min , Ju Xiaobing , Tan Ruoyun 
Published: February 2025

BackgroundChronic allograft dysfunction (CAD) stands as a critical factor that limits the long-term viability of transplanted kidneys. Ferroptosis is an iron-dependent form of programmed cell death increasingly linked to chronic fibrosis. However, the mechanism by which ferroptosis contributes to the onset and progression of CAD remains unclear.MethodsThis study analyzed transcriptome data from renal transplant biopsy samples in the Gene Expression Omnibus (GEO), through clinical samples, animal models, and cell experiments, this study investigated the mechanism by which Caveolin-1 (CAV1) promotes CAD through the regulation of the ferroptosis pathway.ResultsThe elevated levels of CAV1 were found to positively correlate with CAD incidence. Clinical and animal model validation confirmed heightened CAV1 expression in CAD. In vitro experiments demonstrated that CAV1 can directly promote chronic renal allograft interstitial fibrosis by regulating ferroptosis in renal tubular epithelial cells; additionally, it can promote epithelial-to-mesenchymal transition (EMT) by secreting Interleukin- 6 (IL-6), thereby further contributing to CAD.ConclusionCAV1 plays a critical role in the development of CAD by promoting EMT and chronic renal allograft interstitial fibrosis through the ferroptosis pathway. Adjusting ferroptosis by altering the expression abundance of CAV1 may become an important method for the prevention and treatment of CAD in the future.
Ultrasensitive interferons quantification reveals different cytokine profile secretion in inflammatory myopathies and can serve as biomarkers of activity in dermatomyositis
Authors: Bolko Loïs , Anquetil Céline , Llibre Alba , Maillard Solène , Amelin Damien , Dorgham Karim , Bondet Vincent , Landon-Cardinal Océane , Toquet Ségolène , Mariampillai Kuberaka , Malatre Samuel , Mahoudeau Alexandrine , Hervier Baptiste , Rodero Mathieu , Gorochov Guy , Duffy Darragh , Benveniste Olivier , Allenbach Yves 
Published: February 2025

ObjectiveThe objective of this study was to evaluate the presence of different types of interferon in idiopathic inflammatory myopathies (IIM) and their subgroups using ultrasensitive cytokine detection techniques (SIMOA) and to assess their potential as activity biomarkers.MethodsDisease activity was measured at the time of serum collection and assessed by manual muscle testing eight (MMT8 score 0-150), muscle enzymes to calculate the Physician Global Assessment (PGA) (0-10). Patients were classified as active if PGA&gt;5.Serum IFN-α and IFN-γ levels was measured using the single molecule array (SIMOA) technique. Serum IFN-β level was measured by Elisa. Correlation between IFN levels and disease activity were performed.ResultsWe included 242 IIM patients and found a good correlation between type I Interferon (IFN) and dermatomyositis disease activity. IFN-α and IFN-β was highly correlated with disease activity (r=0.76 and r=0,58). To evaluate whether the different types of Interferons could serve as biomarkers of activity, we generated ROC curves. Patients with active DM had a higher median IFN-α level (0.49 pg/ml [0.1-3.7]) compared with non-active patients (0.03 pg/ml [0.01-0.07] p&lt;0.05). The area under the curve was 0.90 IC95 (0.76-0.97) p&lt;0.05. Furthermore, Myositis-specific antibodies appear to be associated with a different secretion profile; patients with anti-MDA 5 antibodies had higher level of IFN-α than most other antibodies (6.58 vs 0.14 p&lt;0.005). NXP2 had higher IFN-β level than patients with Tif1γ antibodies.ConclusionSerum IFN-α level measured by SIMOA is a reliable biomarker of DM activity. Myositis-specific antibodies appear to be associated with a different secretion profile. This data needs to be confirmed in order to select the good therapeutics strategies in DM.
Exploring patient preferences for intraocular lenses selection in age-related cataract: a discrete choice experiment study
Authors: Zhu Qiaolin , Sun Qianqian , Huo Yujia , Yang Xiaoling , Huang Hehe , Qian Shanshan , Lin Wenjing , Yan Wentao 
Published: February 2025

IntroductionThis study investigates the preferences of cataract patients in East China regarding intraocular lenses (IOLs).MethodsA Discrete Choice Experiment (DCE) was conducted using a questionnaire that included various IOLs attributes. Participants made choices based on different combinations of these attributes, and the data were analyzed using multinomial logit models (MNL) and latent class analysis (LCA) to identify preference heterogeneity.ResultsA total of 200 cataract patients (mean age 66.2 years, 58.5% female) participated in the study. The most influential factors in IOL selection were cost, followed by presbyopia correction, with a preference for multifocal IOLs (trifocal and bifocal), spherical aberration correction, and astigmatism correction. High cost and a higher probability of adverse visual phenomena negatively affected preferences. The inclusion of blue-blocking functionality and the surgeon’s recommendation had minimal influence on patient choice. LCA revealed three distinct preference groups: Class 1 (“Aberration Correction Seekers”) preferred aspheric IOLs, Class 2 (“Presbyopia and Blue-Blocking Enthusiasts”) favored multifocal IOLs, and Class 3 (“Astigmatism and Cost-Sensitive Patients”) preferred toric IOLs. Multinomial logistic regression analysis further showed that male patients were more likely to choose toric IOLs, while individuals with higher education levels were significantly less likely to prefer multifocal IOLs.ConclusionThis study highlights significant heterogeneity in cataract patient preferences for IOLs attributes. Cost was the most critical factor, followed by presbyopia and aberration correction. Men favored toric IOLs and exhibited sensitivity to cost, while highly educated individuals preferred multifocal IOLs less. These findings underscore the need for personalized IOLs recommendations and signal opportunities for innovation and customization in the IOLs industry.
Abdominal wall hematoma as a complication of drainage after laparoscopic cholecystectomy: a case report
Authors: Li Juntao , Liu Zixiong , Li Jia , Cheng Wei 
Published: February 2025

BackgroundAbdominal wall hematoma represents a potential postoperative complication that requires prompt identification and appropriate management. This case report retrospectively analyzes a patient who developed an abdominal wall hematoma associated with a drainage tube and puncture site following laparoscopic cholecystectomy at our hospital. The clinical characteristics, treatment modalities, and relevant literature are reviewed to highlight strategies for the prevention and management of postoperative hematomas, with the aim of providing valuable insights for clinical practice. We managed a patient who had undergone laparoscopic cholecystectomy for gallstones complicated by cholecystitis. On the first postoperative day, a hematoma developed at the site of the abdominal drainage tube insertion. Despite initial attempts at hemostasis through abdominal wall compression, these measures proved ineffective, necessitating the use of a urinary catheter balloon for effective compression hemostasis.Case presentationWe treated a patient who had undergone laparoscopic cholecystectomy for gallstones complicated by cholecystitis. On the first postoperative day, a hematoma developed at the site of the abdominal drainage tube insertion. Despite initial attempts at hemostasis using abdominal wall compression, these measures were ineffective, necessitating the use of a urinary catheter balloon for effective compression hemostasis.ConclusionThe urinary catheter balloon tamponade was effectively employed postoperatively to achieve hemostasis for the hematoma at the abdominal wall drainage site. It provides a viable alternative for early intervention in hematoma management.
Diagnostic investigation, intervention, and outcome for post-subtotal gastrectomy patients who present with jaundice
Authors: Wang Duan , Zhu Liang , Kong Fanyi , Pan Yingyu , Liu Wei , Wang Xuan , Pan Weidong , Cao Jian , Xu Qiang , Wu Dong 
Published: February 2025

PurposeEndoscopic retrograde cholangiopancreatography (ERCP) is a useful diagnostic and interventional tool in patients with obstructive jaundice. In patients who had subtotal gastrectomy, however, the implementation of ERCP has become more difficult. This study aims to investigate the accuracy of contrast-enhanced CT, MRI/MRCP and PET/CT in lesion localization, characterization, and extent evaluation in post-subtotal gastrectomy patients who present with obstructive jaundice. The interventional methods for biliary drainage, their success rate and patient outcome were also investigated.MethodsElectronic medical records were reviewed to identify patients hospitalized for obstructive jaundice at Peking Union Medical College Hospital, who had previously undergone subtotal gastrectomy. The clinical information, imaging and interventional examination data of those patients were retrospectively collected.ResultsBetween 2018 and 2023, 36 patients with previous subtotal gastrectomy were hospitalized for ob-structive jaundice at our hospital. The majority of lesions were malignant, including 19 gastric cancer recurrence (47.5%), and 12 other malignancies (30.0%). Benign lesions included inflammatory biliary stricture, biliary stones, and IgG4-related disease. The three imaging modalities had similar performance in lesion localization and characterization, whereas PET/CT showed higher accuracy compared to MR and CT in detecting extensive disease (92.8% vs. 83.3% vs. 60.0%). Percutaneous transhepatic cholangial drainage was applied more frequently than ERCP and surgery (69.4% vs. 25.0% vs. 5.5%), and there was no significant difference concerning technical and clini-cal success rate and complication.ConclusionGastric cancer recurrence and newly-developed pancreaticobiliary malignancies were the main causes of obstructive jaundice in patients who had subtotal gastrectomy. PET/CT was superior to MRI/MRCP and contrast-enhanced CT in determining lesion extensiveness. Percutaneous transhepatic cholangial drainage (PTCD) was the preferred method for managing obstructive jaundice. Despite the effectiveness of interventions, a significant number of patients experienced short-term disease progression.
Adherence to the Canadian 24-hour movement guidelines and vision impairment in children and adolescents: a cross-sectional study
Authors: Zhong Haoxi , Zhu Huimin , Jiang Mingjie , Mu Jingfeng 
Published: February 2025

ObjectivesTo investigate the associations between adherence to the Canadian 24-hour movement guidelines—covering physical activity (PA), screen time (ST), and sleep duration (SD)—and vision impairment, specifically myopia and myopic anisometropia, among children and adolescents in Shenzhen, China.MethodsA cross-sectional study was conducted in 2022 with 4,649 participants. Adherence to the guidelines was assessed using self-reported PA, ST, and SD measures, while vision impairment was clinically evaluated. Logistic regression models were used to analyze the associations, adjusting for sociodemographic factors.ResultsAmong the participants, 48.63% were diagnosed with myopia and 11.01% had myopic anisometropia. Meeting the ST guideline was associated with a reduced risk of myopia (aOR = 0.86, 95% CI = 0.76-0.98) and myopic anisometropia (aOR = 0.78, 95% CI = 0.64-0.95). Meeting both PA and ST guidelines further reduced the odds of myopia (aOR = 0.73, 95% CI = 0.56-0.97) and myopic anisometropia (aOR = 0.60, 95% CI = 0.41-0.89). Meeting all three guidelines (PA, ST, and SD) significantly reduced the odds of myopia (aOR = 0.71, 95% CI: 0.53–0.93) and showed a trend toward reduced risk of anisometropia (aOR = 0.69, 95% CI: 0.47–1.02), compared to those who met none. Meeting two guidelines also significantly reduced the risk of myopia (aOR = 0.76, 95% CI: 0.59–0.97) and anisometropia (aOR = 0.71, 95% CI: 0.51–1.00).ConclusionAdherence to the 24-hour movement guidelines, particularly meeting the ST and PA recommendations, was associated with a lower risk of myopia and myopic anisometropia. These findings highlight the importance of promoting balanced lifestyle behaviors, such as limiting screen time and encouraging physical activity, to mitigate vision impairment among children and adolescents.
Functional and effective EEG connectivity patterns in Alzheimer’s disease and mild cognitive impairment: a systematic review
Authors: Paitel Elizabeth R. , Otteman Christian B. D. , Polking Mary C. , Licht Henry J. , Nielson Kristy A. 
Published: February 2025

BackgroundAlzheimer’s disease (AD) might be best conceptualized as a disconnection syndrome, such that symptoms may be largely attributable to disrupted communication between brain regions, rather than to deterioration within discrete systems. EEG is uniquely capable of directly and non-invasively measuring neural activity with precise temporal resolution; connectivity quantifies the relationships between such signals in different brain regions. EEG research on connectivity in AD and mild cognitive impairment (MCI), often considered a prodromal phase of AD, has produced mixed results and has yet to be synthesized for comprehensive review. Thus, we performed a systematic review of EEG connectivity in MCI and AD participants compared with cognitively healthy older adult controls.MethodsWe searched PsycINFO, PubMed, and Web of Science for peer-reviewed studies in English on EEG, connectivity, and MCI/AD relative to controls. Of 1,344 initial matches, 124 articles were ultimately included in the systematic review.ResultsThe included studies primarily analyzed coherence, phase-locked, and graph theory metrics. The influence of factors such as demographics, design, and approach was integrated and discussed. An overarching pattern emerged of lower connectivity in both MCI and AD compared to healthy controls, which was most prominent in the alpha band, and most consistent in AD. In the minority of studies reporting greater connectivity, theta band was most commonly implicated in both AD and MCI, followed by alpha. The overall prevalence of alpha effects may indicate its potential to provide insight into nuanced changes associated with AD-related networks, with the caveat that most studies were during the resting state where alpha is the dominant frequency. When greater connectivity was reported in MCI, it was primarily during task engagement, suggesting compensatory resources may be employed. In AD, greater connectivity was most common during rest, suggesting compensatory resources during task engagement may already be exhausted.ConclusionThe review highlighted EEG connectivity as a powerful tool to advance understanding of AD-related changes in brain communication. We address the need for including demographic and methodological details, using source space connectivity, and extending this work to cognitively healthy older adults with AD risk toward advancing early AD detection and intervention.
NRBF2 plays a crucial role in the acquisition process of learning and memory, independent of the Vps34 complex
Authors: Wu Songfen , Zhuang Haicai , Zhou Xidan , Li Kuan 
Published: February 2025

IntroductionNRBF2, a component of autophagy-associated PIK3C3/VPS34-containing phosphatidylinositol 3-kinase complex, plays a crucial role in learning and memory processes, yet its specific impact on memory and the underlying molecular mechanisms remains unclear.MethodsHere, we utilized NRBF2 knockout mice to examine its influence on the time course of fear memory. Employing quantitative PCR, Western blot analysis, behavioral tests, and electrophysiology, we investigated the mechanisms through which NRBF2 affects memory processing.ResultsWe observed an increase in Nrbf2 mRNA levels at 6 and 12 h, and protein levels at 6 h post fear conditioning. Depletion of NRBF2 impaired memory acquisition, short-term, and long-term memory without causing any anxiety-like behavior. Interestingly, inhibition of Vps34 and autophagy by SAR405 disrupted fear memory consolidation, while leaving memory acquisition, short-term memory, and long-term potentiation (LTP) unaffected. Our results suggested that NRBF2 deletion impaired memory acquisition through an autophagy-independent pathway and provided novel insights into the role of NRBF2 in the central nervous system.DiscussionThis study offer new insights into the role of NRBF2 and highlight the potential of targeting NRBF2 as a therapeutic strategy for addressing cognitive deficits associated with various disorders.
Role of microRNA in the risk stratification of ischemic strokes
Authors: Al-Jehani Hosam M. , Mousa Ahmed Hafez , Alhamid May Adel , Al-Mufti Fawaz 
Published: February 2025

BackgroundIschemic stroke is a major cause of death and morbidity, and risk classification is essential for predicting therapeutic outcomes. MicroRNAs may be useful indicators for risk stratification, as they control gene expression and influence physiological and pathological processes.MethodologyA systematic strategy was developed to search relevant material using databases like PubMed, Scopus, and Web of Science. Selection criteria included human research, a certain date, or categories of studies. Data extraction, synthesis, and analysis were carried out to find trends, similarities, and differences among the chosen studies. The study’s design, sample size, methodology, statistical analysis, and any potential biases or restrictions from the selected reference papers were also taken into account.Results and findingsMicroRNA is an important biomarker for risk stratification in Ischemic Strokes. It can be used to identify Stroke-Specific microRNA Signatures, identify diagnostic and prognostic values, and regulate Vascular Inflammation, Endothelial Dysfunction, and Thrombus Formation and Resolution. It also has potential therapeutic applications.ConclusionMicroRNAs have emerged as promising biomarkers for predicting stroke risk, severity of strokes, and clinical outcomes. They can be used to predict the severity of a stroke and aid clinicians in making treatment decisions.
Association between serum total bilirubin levels and 28-day all-cause mortality after intracerebral hemorrhage
Authors: Qiu Dachang , Li Guangwei , Dong Yongfei 
Published: February 2025

BackgroundIntracerebral hemorrhage (ICH) is associated with high mortality and morbidity rates. Although some studies have indicated a correlation between serum bilirubin levels and ICH severity, evidence of the relationship between serum total bilirubin (TBIL) and ICH outcomes remains lacking.MethodsA total of 914 patients from the Medical Information Mart for Intensive Care IV database met the eligibility criteria and were included in the study. The patients were categorized into two groups based on whether they survived for 28 days following admission to hospital. The association between serum TBIL levels and 28-day survival in patients with ICH was investigated using Spearman’s correlation analysis and restricted cubic splines. The effect of serum TBIL levels on survival time and rate in the 28-day period was analyzed using Kaplan–Meier curves and restricted mean survival times. Univariate Cox regression, least absolute shrinkage and selection operator regression, and multivariate Cox regression were used to identify risk factors associated with 28-day all-cause mortality. Finally, subgroup analysis was performed to verify the stability of the association between serum TBIL levels and 28-day all-cause mortality in patients with ICH.ResultsA negative relationship was revealed between TBIL levels and survival (p &lt; 0.001, correlation = −0.174). Restricted cubic spline analysis revealed a nonlinear link between mean serum TBIL levels and 28-day all-cause mortality (p for nonlinear = 0.001). Patients with ICH and higher serum TBIL levels had significantly reduced survival times and rates compared with those with lower serum TBIL levels (p &lt; 0.001). Serum TBIL level was identified as a significant risk factor for 28-day all-cause mortality in patients with ICH (hazard ratio [95% confidence interval] = 1.121 [1.063–1.182], p &lt; 0.001). Subgroup analyses revealed that the assessed variables had no influence on the association between serum TBIL levels and 28-day all-cause mortality.ConclusionHigher serum TBIL levels are associated with a greater risk of mortality within 28 days in patients with ICH, whereas lower serum TBIL levels are associated with prolonged survival.
Brain plasticity associated with prolonged shooting training: a multimodal neuroimaging investigation from a cross-sectional study
Authors: Zhang Keying , Zhao Tao , Ding Yu , Cheng Jia , Cao Chunmei 
Published: February 2025

BackgroundAlthough training has been recognized as a potential contributor to neuroplasticity in athletes, the impact of prolonged shooting training on human brain plasticity remains unclear in the existing literature.MethodsIn this cross-sectional study, we used a multimodal neuroimaging analysis, including the analysis of functional blood oxygenation level-dependent (BOLD) magnetic resonance imaging (MRI) images, structural T1-weighted MRI images, and diffusion MRI images, to systematically identify differences between elite shooters and normal controls.ResultsThe results showed that compared to male normal controls, male elite shooters had higher regional homogeneity (ReHo) in the frontal lobe, parietal lobe, precuneus, thalamus, and cingulate gyrus, as well as higher functional connectivity between the medial frontal cortex (MedFC) and temporooccipital middle temporal gyrus (toMTG). Male elite shooters also showed higher cortical thickness in the right inferior temporal lobe; lower fractional anisotropy (FA) values in the right superior longitudinal fasciculus (SLF), right inferior fronto-occipital fasciculus (IFF), and right anterior thalamic radiation (ATR); lower axial diffusivity (AD) value in forceps minor and left ATR; and lower structural connectivity between right putamen and right inferior parietal cortex (IPC), right IPC and right paracentral cortex, and right paracentral cortex and right superior parietal cortex (SPC).ConclusionElite male shooters exhibited optimized resting-state functional activity, functional connectivity, and morphological features compared to normal controls. Prolonged shooting training may contribute to enhancing the brain’s functional and structural plasticity related to motor control, attentional focus, and emotion regulation in male shooters; however, similar changes have not been observed in female shooters.
Application of oral nutritional supplements to control body weight loss in postoperative patients suffering from solid tumors: a systematic review and meta-analysis
Authors: Liu Ying , Wu Zhen , Shao Tingting , Zheng Wanzhen , Huang Jing 
Published: February 2025

ObjectiveThis study aims to summarize the impact of oral nutritional supplements (ONSs) on mitigating body weight loss (BWL) in patients following surgical treatment for solid tumors.MethodsA systematic and comprehensive search of four major publicly available databases was conducted up to May 2024 to identify studies for inclusion in the analysis. Data from eligible studies were extracted, and pooled mean differences (MD) along with their 95% confidence intervals (CIs) for BWL were computed.ResultsA total of 12 randomized controlled trials (RCTs) with 2,268 participants were finally included. The group receiving oral nutritional supplements demonstrated a statistically significant reduction in weight loss compared to the control group, with a mean difference of 1.11 (95% CI: 0.52–1.70), an I2 statistic of 97.0%, and a p-value less than 0.01.ConclusionThe meta-analysis provide evidence that ONSs effectively reduce BWL in postoperative patients with solid tumors. Additionally, ONS with lower daily caloric intake demonstrated superior efficacy in reducing BWL.