bluuebunny/crossref_metadata_2025_split huggingface dataset:
Long‐term outcome of porcine skin graft in surgical treatment of recurrent pelvic organ prolapse. An open randomized controlled multicenter study
Authors: DAHLGREN EVA , KJØLHEDE PREBEN ,   on behalf of the RPOP‐PELVICOL Study Group
Published: September 2011

AbstractObjective. To determine the long‐term objective and subjective outcomes of use of a porcine skin graft (Pelvicol™) compared with conventional colporrhaphy in recurrent pelvic organ prolapse surgery and to analyze risk factors and safety. Design. Open randomized controlled multicenter study. Setting. Eight Swedish hospitals. Population. 135 consecutive women with recurrent cystocele and/or rectocele admitted for vaginal prolapse surgery; 132 completed the study, 64 were randomly allocated to receive conventional colporrhaphy and 68 to Pelvicol. Methods. Conventional anterior and posterior colporrhaphy and colporrhaphy with use of Pelvicol™ mesh reinforcement. Clinical evaluation by means of pelvic organ prolapse quantification (POP‐Q) and symptom questionnaire preoperatively, three months and three years postoperatively. Main outcome measures. Anatomical and subjective outcome.  Recurrence was defined as POP‐Q≥stage 2. Results. At three‐month follow‐up, early recurrence/surgical failures occurred significantly more often in the Pelvicol™ group, but at the three‐year follow‐up the recurrence rates were similar. The recurrence rates in the anterior compartment were 57–62% and 44–23% in the posterior compartment for the colporrhaphy and Pelvicol™ groups, respectively. Symptoms were substantially and equally reduced in the two groups after surgery. Sexual activity and function did not seem to be affected adversely in any group. The complication rate was low. Risk factors for anatomical recurrence were age, body mass index and preoperative stage of the prolapse. Conclusions. With the surgical technique used in this study, Pelvicol™ did not provide advantages over conventional colporrhaphy in recurrent pelvic organ prolapse concerning anatomical and subjective outcomes.
First trimester prediction of small‐ and large‐for‐gestation neonates by an integrated model incorporating ultrasound parameters, biochemical indices and maternal characteristics
Authors: PAPASTEFANOU IOANNIS , SOUKA ATHENA P. , PILALIS ATHANASIOS , ELEFTHERIADES MAKARIOS , MICHALITSI VASILIKI , KASSANOS DEMETRIOS 
Published: September 2011

AbstractObjective. To identify maternal/pregnancy characteristics, first trimester ultrasound parameters and biochemical indices which are significant independent predictors of small‐for‐gestational age (SGA) and large‐for‐gestational age (LGA) neonates. Design. Retrospective cross‐sectional study. Setting. Two fetal Medicine Units. Population. 4 702 singleton pregnancies presenting for screening for chromosomal abnormalities by nuchal translucency and maternal serum biochemistry at 11–14 weeks. Methods. Reference ranges for birthweight applied to our population were constructed by the Royston and Wright method. Multiple logistic regression was applied to develop first trimester prediction models for SGA and LGA. Main outcome measures. Birth of SGA or LGA neonate. Results. Maternal height, parity, smoking, assisted conception, delta crown–rump length, delta nuchal translucency, free beta human chorionic gonadotrophin and pregnancy‐associated plasma protein‐A were significant independent predictors of SGA. Maternal weight and height, smoking, delta crown–rump length and delta nuchal translucency were significant independent predictors of LGA. Models for SGA (AUC=0.7296, CI: 0.69–0.76, p<0.0001) and LGA (AUC=0.6901, CI: 0.65–0.72, p<0.0001) were derived, applicable to routine obstetric population at low risk for these conditions. For 20% screen positive rate the modeling achieves sensitivities of about 55% for SGA and 48% for LGA neonates. Conclusion. Prediction for birthweight deviations is feasible using data available at the routine 11–14 weeks’ examination. Delta CRL and delta nuchal translucency were significant independent predictors for both SGA and LGA.
The potential effects of cholecalciferol on bone regeneration in dogs
Authors: Hong Hsiang‐Hsi , Chou Ting‐An , Yang Jen‐Chang , Chang Chee‐Jen 
Published: September 2011

AbstractObjectivesThe aim of this investigation was to evaluate the potential effects of the systemically delivered combination of calcium supplementation and cholecalciferol and of the locally applied biphasic calcium alloplast on the surgically produced alveolar sockets at the early healing stage in a dog model.Materials and methodsThe mandibular pre‐molars of nine Beagle dogs were extracted first. Three months later, four standardized sockets with a 4 mm in diameter and 6 mm deep cylinder were created bilaterally at healed extraction sites. The sockets on one side were grafted with biphasic calcium phosphate alloplast, whereas the defects on the other side were left un‐grafted. The dogs were then randomly divided into two groups; five dogs received oral calcium and cholecalciferol combination (Vit.D/Ca) and were assigned to the test group (Vit.D/Ca Group). The other four dogs without Vit.D/Ca supplement were distributed to the control group (Non‐Vit.D/Ca Group). The bone density (Density) and the implant stability quotient (ISQ) at prepared sites were measured 4 weeks later. The drawn bone cores were examined by a histomorphometric analysis for measurement of new bone formation (NBF). The amount of vertical ridge resorption (VRR) was evaluated.ResultsThe Vit.D/Ca‐treated subjects revealed significantly more NBF (P < 0.05), higher bone density (P < 0.05) and significantly less vertical ridge reduction (P < 0.05) in the healing sockets than those without Vit.D/Ca treatment. The non‐grafted sockets demonstrated significantly more NBF (P < 0.05), higher bone density (P < 0.05), better ISQ value (P < 0.05) and more vertical ridge reduction (P < 0.05) than those in the grafted sockets. There was no significant difference between the serum data determined before and 4 weeks after experiment in Vit.D/Ca Group or Non‐Vit.D/Ca Group. However, the changed value (post‐op vs. pre‐op) between the two groups was significant in the serum level of phosphate and parathyroid hormone (P = 0.05).ConclusionThe results of the present study indicated that the examined calcium phosphate alloplast may perform a function in alveolar ridge preservation while reducing the potential of NBF potential in the healing extraction socket. Also, the combination of calcium supplementation and cholecalciferol may have systemic effects on accelerating bone regeneration.
Retracted: Peri‐implant bone reactions to immediate implants placed at different levels in relation to crestal bone. Part II: a pilot study in dogs
Authors: Negri Bruno , Calvo‐Guirado José Luis , Ramírez‐Fernández María Piedad , Maté Sánchez‐de Val José , Guardia Javier , Muñoz‐Guzón Fernando 
Published: September 2011

AbstractPurpose:The aim of the present study was to evaluate bone‐to‐implant contact (BIC) and bone remodeling dynamics after immediate implant placement at different levels in relation to the crestal bone with two different implant designs in beagle dogs.Materials and methods:The mandibular second, third and fourth premolars of six beagle dogs were extracted bilaterally and three implants were immediately placed in the hemi‐arches of each dog. Randomly, three cylindrical and three tapered implants were inserted crestally (control group) or 2 mm subcrestally (test group). Both groups were treated with a minimal mucoperiosteal flap elevation approach. A gap from the buccal cortical wall to the implant was always left. Three dogs were allowed a 4‐week submerged healing period and the other three an 8‐week submerged healing period. The animals were sacrificed and samples were obtained. Biopsies were processed for ground sectioning. Histomorphometric analysis was carried out in order to compare BIC, de novo bone formation and bone remodeling.Results:All implants osseointegrated clinically and histologically. Healing patterns examined microscopically at 4 and 8 weeks for both groups (crestal and subcrestal) yielded similar qualitative bone findings. The total BIC mean value for the crestal group was 36.48 ± 3.4% and for the subcrestal group was 41.46 ± 4.2%. The mean percentage of newly formed BIC was greater with the cylindrical implant design (46.06 ± 1.09%) than with the tapered design (33.89 ± 1.72%). There was less bone resorption in the subcrestal group (test) than crestal group (control).Conclusion:These findings suggest that apical positioning of the top of the implant does not jeopardize bone crest and peri‐implant tissue remodeling. However, less resorption of the lingual crest may be expected when implants are placed 2 mm subcrestally. Moreover, higher BIC values were found in cylindrical implants placed subcrestally.
Immediate rehabilitation of the edentulous mandible with screw type implants: results after up to 10 years of clinical function
Authors: Heschl Alexander , Payer Michael , Platzer Susanne , Wegscheider Walther , Pertl Christof , Lorenzoni Martin 
Published: September 2011

AbstractObjectivesThe aim of this prospective case series was to evaluate the results of an immediate loading concept using four Xi VE S plus implants in the edentulous mandible, after a period of up to 10 years of clinical function.Material and methodsThirty patients were treated with four implants each placed interforaminally and provisionally restored within 1 week. Radiographic bone levels, condition of the peri‐implant mucosa, implant survival and success were recorded annually from implant insertion (baseline) up to 10 years after final restoration.ResultsA total of 120 Xi VE S plus implants were placed in the interforaminal region. A significant coronal bone loss of 1.80 mm (SD ± 0.65) was recorded within the first 8 years of function (P < 0.001). Within the next years no further significant increase of bone resorption was observed. The mean values of the plaque, calculus, bleeding and mucosal indices and probing depth remained low throughout this period. All implants were inserted with an insertion torque of more than 32 N cm. Two losses (1.7%) occurred prior to permanent restoration (1 and 3 months post‐insertion), resulting in a survival rate of 98.3% over the entire observation period. Four implants were recorded as failures due to excessive bone resorption, resulting in an overall success rate of 95%.ConclusionsThe results of this study indicate that in selected patients immediate restoration of dental implants in the edentulous mandible will achieve a clinically predictable outcome.
Geographic origin and past climatic experience influence the response to late spring frost in four common grass species in central Europe
Authors: Kreyling Juergen , Thiel Daniel , Simmnacher Katrin , Willner Evelin , Jentsch Anke , Beierkuhnlein Carl 
Published: September 2011

Late spring frost events can affect vegetation. The response of grassland species, however, is generally unknown. We explore the late‐frost sensitivity of four common European grass species and investigate whether these species exhibit local adaptations to late frost on a continental scale and whether past climatic experience influences late frost sensitivity.Ecotypes of Arrhenatherum elatius, Alopecurus pratensis, Festuca pratensis, and Holcus lanatus from Spain, Italy, Bulgaria, Hungary, Sweden and Germany were exposed to late frost after drought and warming manipulations in the preceding growing season in a common garden experiment.Late frost reduced the productivity of the grasses on average by 20%. Ecotypes differed in their late‐frost sensitivity in three of the four species and local adaptations to late frost were identified. Previous exposure to drought and warming caused differences in late‐frost sensitivity in some cases.The impact of late frost events may increase in a warmer world due to an earlier onset of growing and no change in timing of late frost events. The history of climatic exposure can alter the performance of plants, possibly through epigenetic mechanisms. Based on the complex response pattern observed, a maximization of genetic diversity is proposed as a promising adaptation strategy against climate change.
Hepatocytes produce tumor necrosis factor‐α and interleukin‐6 in response to Porphyromonas gingivalis
Authors: Takano M. , Sugano N. , Mochizuki S. , Koshi R. N. , Narukawa T. S. , Sawamoto Y. , Ito K. 
Published: September 2011

Takano M, Sugano N, Mochizuki S, Koshi RN, Narukawa TS, Sawamoto Y, Ito K. Hepatocytes produce tumor necrosis factor‐α and interleukin‐6 in response to Porphyromonas gingivalis. J Periodont Res; 2012; 47: 89–94. © 2011 John Wiley & Sons A/SBackground and Objective:  The liver plays a major role in clearing systemic bacterial infections. In addition, inflammatory cytokines produced in the liver play a critical role in systemic cytokine levels. The aim of this study was to investigate the production of tumor necrosis factor‐α (TNF‐α) and interleukin‐6 (IL‐6) by hepatocytes in response to periodontal pathogens.Material and Methods:  The mouse hepatic carcinoma cell line Hepa‐1.6 and the mouse macrophage‐like cell line RAW 264 were co‐cultured in Transwell insert plates. Cells were stimulated with bacterial extracts prepared from Porphyromonas gingivalis and the induction of TNF‐α and IL‐6 was measured using real‐time PCR and ELISA.Results:  After stimulation with bacteria, the induction of TNF‐α and IL‐6 was observed in RAW 264 cells and Hepa‐1.6 cells. Significant reduction of TNF‐α mRNA expression in Hepa‐1.6 cells was observed after treatment with antibody to TNF‐α.Conclusion:  The results obtained in the present study show that P. gingivalis extract induces TNF‐α and IL‐6 in an in vitro liver model and that macrophage‐derived TNF‐α mediates the induction of TNF‐α in hepatocytes.
Early microbial succession in redeveloping dental biofilms in periodontal health and disease
Authors: Teles F. R. , Teles R. P. , Uzel N. G. , Song X. Q. , Torresyap G. , Socransky S. S. , Haffajee A. D. 
Published: September 2011

Teles FR, Teles RP, Uzel NG, Song XQ, Torresyap G, Socransky SS, Haffajee AD. Early microbial succession in redeveloping dental biofilms in periodontal health and disease. J Periodont Res 2012; 47: 95–104. © 2011 John Wiley & Sons A/SBackground and Objective:  The development of dental biofilms after professional plaque removal is very rapid. However, it is not clear whether most bacterial species return at similar rates in periodontally healthy and periodontitis subjects or if there are differences in bacterial recolonization between supragingival and subgingival biofilms in periodontal health and disease.Material and Methods:  Supragingival and subgingival plaque samples were taken separately from 28 teeth in 38 healthy and 17 periodontitis subjects immediately after professional cleaning. Samples were taken again from seven teeth in randomly selected quadrants after 1, 2, 4 and 7 d of no oral hygiene and analyzed using checkerboard DNA–DNA hybridization. The percentage of DNA probe counts were averaged within subjects at each time‐point. Ecological succession was determined using a modified moving‐window analysis.Results:  Succession in supragingival biofilms from subjects with periodontitis and from healthy individuals was similar. At 1 d, Streptococcus mitis and Neisseria mucosa showed increased proportions, followed by Capnocytophaga gingivalis, Eikenella corrodens, Veillonella parvula and Streptococcus oralis at 1–4 d. At 4–7 d, Campylobacter rectus, Campylobacter showae, Prevotella melaninogenica and Prevotella nigrescens became elevated. Subgingival plaque redevelopment was slower and very different from supragingival plaque redevelopment. Increased proportions were first observed for S. mitis, followed by V. parvula and C. gingivalis and, at 7 d, by Capnocytophaga sputigena and P. nigrescens. No significant increase in the proportions of periodontal pathogens was observed in any of the clinical groups or locations.Conclusion:  There is a defined order in bacterial species succession in early supragingival and subgingival biofilm redevelopment after professional cleaning.
Correlation of cytomegalovirus and human herpesvirus 7 with CD3+ and CD3+CD4+ cells in chronic periodontitis patients
Authors: Thomasini R. L. , Bonon S. H. , Durante P. , Costa S. C. B. 
Published: September 2011

Thomasini RL, Bonon SH, Durante P, Costa SCB. Correlation of cytomegalovirus and human herpesvirus 7 with CD3

+

and CD3

+

CD4

+

cells in chronic periodontitis patients. J Periodont Res 2012; 47: 114–120. © 2011 John Wiley & Sons A/SBackground and Objective:  Human chronic periodontitis is an inflammatory process characterized by dense accumulation of immune cells in the periodontal tissue. The periodontitis can lead to loss of teeth in the patient and the pathogenesis of this disease is not completely known. This study tested the hypothesis that chronic periodontitis‐affected sites can harbor betaherpesviruses and that viruses are linked to a profile of the inflammatory infiltrate.Material and Methods:  Biopsies of periodontal tissue were taken from periodontitis‐affected patients and from healthy subjects. Immunohistochemistry was performed to count CD19+ B cells, CD3+ total T cells, T‐CD4+ and T‐CD8+ cell subsets, and PCR was performed to detect cytomegalovirus and human herpesvirus 6 and 7 in the samples. One slide of each sample was stained with Giemsa for histopathological examination and to evaluate the quality of the cellular infiltrate.Results:  As expected, tissues collected from healthy subjects presented no significant level of inflammatory infiltration and were therefore excluded from immunostaining procedures. Results showed that CD19+ B cells were in higher number than CD3+ T cells in the periodontitis‐affected tissue, but this was not statistically significant. The T‐CD4+ lymphocyte subset was significantly higher than the T‐CD8+ lymphocyte subset (p = 0.004) in the samples. Cytomegalovirus and human herpesvirus 7 were found at periodontitis‐affected sites, but not in tissue collected from healthy subjects (p = 0.04 and p = 0.04, respectively). Human herpesvirus 6 was rarely detected. We found a correlation between cytomegalovirus and lower CD19+/CD3+ ratios (ratio < 0.9, p = 0.003) and between human herpesvirus 7 and lower CD19+/CD3+ ratios (ratio < 0.9, p = 0.003) and higher CD4+/CD8+ ratios (ratio > 1.1, p = 0.002).Conclusion:  This study shows that cytomegalovirus and human herpesvirus 7 can be present at periodontitis‐affected sites but are uncommon at healthy periodontal sites. Moreover, our data suggest that cytomegalovirus can be related to an inflammatory infiltrate with predominance of CD3+ T cells, whereas human herpesvirus 7 can be associated with an infiltrate with predominance of T‐CD4+ cells. However, further studies are necessary to support this hypothesis. Herpesviruses could play a role in human chronic periodontitis by modulation of the T cell response.
Targeted 307 nm UVB‐phototherapy in psoriasis. A pilot study comparing a 307 nm excimer light with topical dithranol
Authors: Wollina Uwe , Koch André , Scheibe Armin , Seme Bernd , Streit Ingolf , Schmidt Wolf‐Dieter 
Published: September 2011

BackgroundPhototherapy is a cornerstone in treatment of moderate‐to‐severe psoriasis. Narrow‐band UVB has been shown to be a potent therapeutic tool. To reduce the potential carcinogenic risk, targeted phototherapy has been developed using excimer lasers or excimer light devices (ELD).ObjectiveThe role of excimer light therapy in practice and modes of action are not completely understood. We wanted to investigate a 307 nm ELD for plaque psoriasis in comparison with topical dithranol therapy twice daily.MethodsWe conducted a pilot trial in 21 adult patients with moderate plaque‐type psoriasis. Two target lesions of comparable size and plaque‐modified Psoriasis Activity and Severity Index (PSI) scores were selected. Lesion A was treated three times using a newly developed 307 nm ELD. Lesion B was treated twice daily with dithranol ointment. The mean period of treatment was 9 days. Clinical evaluation included PSI scores, safety, time needed to treat, and patient's satisfaction. In addition, fluorescence‐remission imaging technique was used for objective evaluation.ResultsBoth treatments improved the PSI score (mean 3.0 points). The treatments were safe but ELD was more convenient for patients. The time needed to treat the target lesion was significantly shorter with ELD. Targeted UVB therapy normalized NADH fluorescence in lesional skin.ConclusionsThe 307 nm excimer light therapy for plaque type psoriasis was equipotent to twice daily topical dithranol. Efficacy, safety, and convenience suggest that targeted UVB therapy with quasi monochromatic light is a new useful treatment option for patients with limited psoriatic plaques.
Disturbance history of an old‐growth sub‐alpine Picea abies stand in the Bohemian Forest, Czech Republic
Authors: Svoboda Miroslav , Janda Pavel , Nagel Thomas A. , Fraver Shawn , Rejzek Jan , Bače Radek 
Published: September 2011

AbstractQuestionsWhat historical natural disturbances have shaped the structure and development of an old‐growth, sub‐alpine Picea abies forest? Are large‐scale, high‐severity disturbances (similar to the recent windthrow and bark beetle outbreaks in the region) within the historical range of variability for this forest ecosystem? Can past disturbances explain the previously described gradient in stand structure that had been attributed to an elevation gradient?LocationŠumava National Park (the Bohemian Forest) of the southwest Czech Republic.MethodsWe reconstructed the site's disturbance history using dendroecological methods in a 20‐ha study plot, established to span an elevation gradient. Growth patterns of 400 increment cores were screened for: (1) abrupt increases in radial growth indicating mortality of a former canopy tree and (2) rapid early growth rates indicating establishment in a former canopy gap.ResultsSpatial and temporal patterns of canopy accession varied markedly over the 20‐ha study area, resulting in disturbance pulses that corresponded to an elevation gradient. On the lower slope of the plot, the majority of the trees reached the canopy during two pulses (1770–1800 and 1820–1840), while most trees on the upper slope accessed the canopy in one pulse (1840–1860). Historically documented windstorms roughly coincide with peaks in our disturbance reconstruction.ConclusionsOur study provides strong evidence that these forests were historically shaped by infrequent, moderate‐ to high‐severity natural disturbances. Our methods, however, could not definitively identify the agent(s) of these disturbances. Nevertheless, the recent mid‐1990s windstorm and the ensuing spruce bark beetle outbreak may provide an analogue for past disturbance, as the duration and severity of these events could easily explain past patterns of growth response and recruitment in our results. Thus, it seems reasonable to assume the interaction of windstorms and bark beetles seen in the contemporary landscape has occurred historically. Finally, our results suggest that the previously documented elevation gradient in forest structure may not be related to elevation per se (lower temperatures and shorter growing season) but rather to changes in disturbance severity mediated by elevation.