bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of physicochemical parameters on in vitro assembly and disassembly kinetics of recombinant triple‐layered rotavirus‐like particles
Authors: Mellado Maria Candida M. , Mena Jimmy A. , Lopes António , Ramírez Octavio T. , Carrondo Manuel J.T. , Palomares Laura A. , Alves Paula M. 
Published: June 2009

AbstractVirus‐like particles constitute potentially relevant vaccine candidates. Nevertheless, their behavior in vitro and assembly process needs to be understood in order to improve their yield and quality. In this study we aimed at addressing these issues and for that purpose triple‐ and double‐layered rotavirus‐like particles (TLP 2/6/7 and DLP 2/6, respectively) size and zeta potential were measured using dynamic light scattering at different physicochemical conditions, namely pH, ionic strength, and temperature. Both TLP and DLP were stable within a pH range of 3–7 and at 5–25°C. Aggregation occurred at 35–45°C and their disassembly became evident at 65°C. The isoelectric points of TLP and DLP were 3.0 and 3.8, respectively. In vitro kinetics of TLP disassembly was monitored. Ionic strength, temperature, and the chelating agent employed determined disassembly kinetics. Glycerol (10%) stabilized TLP by preventing its disassembly. Disassembled TLP was able to reassemble by dialysis at high calcium conditions. VP7 monomers were added to DLP in the presence of calcium to follow in vitro TLP assembly kinetics; its assembly rate being mostly affected by pH. Finally, DLP and TLP were found to coexist under certain conditions as determined from all reaction products analyzed by capillary electrophoresis. Overall, these results contribute to the design of new strategies for the improvement of TLP yield and quality by reducing the VP7 detachment from TLP. Biotechnol. Bioeng. 2009; 104: 674–686 © 2009 Wiley Periodicals, Inc.
Quantitative correlation between mRNA secondary structure around the region downstream of the initiation codon and translational efficiency in Escherichia coli
Authors: Seo Sang Woo , Yang Jina , Jung Gyoo Yeol 
Published: June 2009

AbstractTranslational efficiency in Escherichia coli is known to be strongly influenced by the secondary structure around the ribosome‐binding site and the initiation codon in the translational‐initiation region of the mRNA. Several quantitative studies have reported that translational efficiency is attributable to effects on ribosome accessibility predominantly caused by the secondary structure surrounding the ribosome‐binding site. However, the influence of mRNA secondary structure around regions downstream of the initiation codon on translational efficiency after ribosome‐binding step has not been quantitatively studied. Here, we quantitatively analyzed the relationship between secondary structure of mRNA surrounding the region downstream of the initiation codon, referred to as the downstream region (DR), and protein expression levels. Modified hairpin structures containing the initiation codon were constructed by site‐directed mutagenesis, and their effects on expression were analyzed in vivo. The minimal folding free energy (ΔG) of a local hairpin structure was found to be linearly correlated with the relative expression level over a range of fourfold change. These results demonstrate that expression level can be quantitatively controlled by changing the stability of the secondary structure surrounding the DR. Biotechnol. Bioeng. 2009; 104: 611–616 © 2009 Wiley Periodicals, Inc.
Refolding of Npro fusion proteins
Authors: Kaar Waltraud , Ahrer Karin , Dürauer Astrid , Greinstetter Sabine , Sprinzl Wolfgang , Wechner Philipp , Clementschitsch Franz , Bayer Karl , Achmüller Clemens , Auer Bernhard , Hahn Rainer , Jungbauer Alois 
Published: June 2009

AbstractThe autoprotease Npro significantly enhances expression of fused peptides and proteins and drives the formation of inclusion bodies during protein expression. Upon refolding, the autoprotease becomes active and cleaves itself specifically at its own C‐terminus releasing the target protein with its authentic N‐terminus. Npro wild‐type and its mutant EDDIE, respectively, were fused N‐terminally to the model proteins green fluorescent protein, staphylococcus Protein A domain D, inhibitory peptide of senescence‐evasion‐factor, and the short 16 amino acid peptide pep6His. In comparison with the Npro wild‐type, the tailored mutant EDDIE displayed an increased rate constant for refolding and cleavage from 1.3 × 10−4 s−1 to 3.5 × 10−4 s−1, and allowed a 15‐fold higher protein concentration of 1.1 mg/mL when studying pep6His as a fusion partner. For green fluorescent protein, the rate constant was increased from 2.4 × 10−5 s−1 to 1.1 × 10−4 s−1 when fused to EDDIE. When fused to small target peptides, refolding and cleavage yields were independent of initial protein concentration, even at high concentrations of 3.9 mg/mL, although cleavage rates were strongly influenced by the fusion partner. This behavior differed from conventional 1st order refolding kinetics, where yield strongly depends on initial protein concentration due to an aggregation reaction of higher order. Refolding and cleavage of EDDIE fusion proteins follow a monomolecular reaction for the autoproteolytic cleavage over a wide concentration range. At high protein concentrations, deviations from the model assumptions were observed and thus smaller rate constants were required to approximate the data. Biotechnol. Bioeng. 2009; 104: 774–784 © 2009 Wiley Periodicals, Inc.
Clinical scale ex vivo manufacture of neutrophils from hematopoietic progenitor cells
Authors: Timmins Nicholas E. , Palfreyman Emma , Marturana Flavia , Dietmair Stefanie , Luikenga Sanna , Lopez Genghis , Fung Yoke Lin , Minchinton Robyn , Nielsen Lars K. 
Published: June 2009

AbstractDose‐intensive chemotherapy results in an obligatory period of severe neutropenia during which patients are at high risk of infection. While patient support with donor neutrophils is possible, this option is restricted due to donor availability and logistic complications. To overcome these problems, we explored the possibility of large scale ex vivo manufacture of neutrophils from hematopoietic progenitor cells (HPC). CD34+ HPC isolated from umbilical cord blood (UCB) and mobilized peripheral blood (mPB) were expanded in serum‐free medium supplemented with stem cell factor, granulocyte colony stimulating factor, and a thrombopoietin peptide mimetic. After 15 days of cultivation a 5,800‐fold expansion in cell number was achieved for UCB, and up to 4,000‐fold for mPB, comprising 40% and 60% mature neutrophils respectively. Ex vivo expanded neutrophils exhibited respiratory burst activity similar to that for donor neutrophils, and were capable of killing Candida albicans in vitro. These yields correspond to a more than 10‐fold improvement over current methods, and are sufficient for the production of multiple neutrophil transfusion doses per HPC donation. To enable clinical scale manufacture, we adapted our protocol for use in a wave‐type bioreactor at a volume of 10 L. This is the first demonstration of a large scale bioprocess suitable for routine manufacture of a mature blood cell product from HPC, and could enable prophylactic neutrophil support for chemotherapy patients. Biotechnol. Bioeng. 2009; 104: 832–840 © 2009 Wiley Periodicals, Inc.
Bond Scrambling and Network Elasticity
Authors: Ciferri Alberto 
Published: June 2009

AbstractDynamic networks (DNs) recently reported in the literature are based on cross‐linked supramolecular chains or on covalent chains with reversible bonds. As originally pointed out by Lehn, these networks should be regarded as dynamic materials exhibiting adaptive features due to continuous scrambling of their bonds and sequences. Results in the recent literature reveal that these networks undergo reversible long‐range deformation resembling that of rubber networks. The present analysis of this process in terms of the theory of composite networks is based on the expectation that the scrambling process should allow rupture of bonds in the undeformed state and their reformation in the stretched state. Accordingly, a permanent set of the resting length of DNs should generally be expected and set materials should retain long‐range elasticity relative to the set state. However, only a limited set is shown by DNs, implying that a strong memory of the initial network topology assists elastic recovery of the original dimensions. The analysis of reported experimental data further reveals that the stress‐strain dependence of dynamic networks accurately follows the classical rubber elasticity theory. In this respect, DNs show better rubber behavior than typical covalent networks. Consistently with theoretical predictions, this surprising finding suggests that bond scrambling relieves local strain constraints on the fluctuations of networks junctions and favors the recovery of the initial network topology. Scrambling therefore allows compliance under stress and enhanced recovery when stress is released. Unprecedented applications of these advanced materials thus become foreseeable.
One‐Pot Template‐Free Synthesis of Monodisperse Zinc Sulfide Hollow Spheres and Their Photocatalytic Properties
Authors: Yu Xiaoxiao , Yu Jiaguo , Cheng Bei , Huang Baibiao 
Published: June 2009

AbstractLittle hollow! Monodisperse ZnS hollow nanospheres (see figure) of about 200 nm in size have been fabricated on a large scale by a hydrothermal method and they show good photocatalytic activity in the decolorization of an aqueous solution of rhodamine B under UV irradiation.magnified imageMonodisperse wurtzite ZnS hollow spheres with diameters of about 200 nm and shells composed of nanoparticles have been successfully synthesized in high yield by a one‐pot template‐free hydrothermal route. The reaction duration, reactant species, and reaction temperature have been shown to play important roles in the formation of ZnS hollow spheres. X‐ray diffraction, scanning and transmission electron microscopy, nitrogen adsorption/desorption, UV/Vis diffuse reflectance spectroscopy, and photoluminescence were used to characterize the products. The results show that all the prepared nanospheres have hexagonal wurtzite structures and exhibit good size uniformity and regularity. A mechanism for the formation of the ZnS hollow spherical structure by localized Ostwald ripening has been proposed based on experimental observations. In addition, studies of the photocatalytic properties of the ZnS hollow spheres by exposure to UV irradiation have demonstrated that they have potential photocatalytic applications. Hydroxyl radicals (.OH) were not detected on the surface of UV‐illuminated ZnS by the photoluminescence technique, which suggests that .OH is not the dominant photo‐oxidant and a photogenerated hole could instead directly participate in the photocatalytic reaction. The prepared ZnS hollow spheres are also of great interest for use in flat displays, sensors, lasers, catalysis, separation technology, biomedical engineering, and nanotechnology.
Supramolecular Alternating Block Copolymers via Metal Coordination
Authors: Yang Si Kyung , Ambade Ashootosh V. , Weck Marcus 
Published: June 2009

AbstractSuprapolymers: The synthesis of symmetrically end‐functionalized polymers in a single step has been developed by means of ring‐opening metathesis polymerization by using a bimetallic ruthenium initiator and functional chain terminators. Self‐assembly of the resulting polymers allows for the formation of supramolecular alternating block copolymers (see figure).magnified imageA bimetallic ruthenium olefin metathesis initiator was synthesized and used to polymerize functionalized norbornenes, affording polymers that are living at both polymer chain‐ends. Using this bis‐ruthenium initiator strategy and combining it with functional chain‐terminators, highly‐efficient syntheses of either SCS‐PdII pincer‐ or pyridine‐functionalized symmetrical telechelic polymers were developed. The terminal functional group incorporation was confirmed by 1H NMR spectroscopy analyses. The telechelic polymers were self‐assembled into block copolymers by means of metal coordination between corresponding terminal recognition units. The self‐assembly process was monitored by 1H NMR spectroscopy revealing nearly quantitative functionalization. The resulting supramolecular block copolymers were further characterized by viscometry and dynamic light scattering.
ChemInform Abstract: The Structure of Cu6Sn5—xSbx — Large Effects of Subtle Doping.
Authors: Lidin Sven , Piao Shu‐Ying 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Lithium Intercalation into β‐Rhombohedral Boron: LiB≈10 or Li30B309?
Authors: Yojteer Natascha , Stauffer Julia , Hillebrecht Harald , Hofmann Kathrin , Panda Monalisa , Albert Barbara 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Structure and Properties of Dimorphic CePdZn.
Authors: Hermes Wilfried , Mishra Ratikanta , Mueller Helen , Johrendt Dirk , Poettgen Rainer 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: New Germanium‐Rich Compounds SrCo5‐xGe9 (x = 0.39 and 0.28) with Optimized Co—Ge Bonding.
Authors: Hlukhyy Viktor , Faessler Thomas F. , Claus Peter 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Structure and Properties of Mixed‐Valence Compound Eu5Zr3S12.
Authors: Jakubcova Petra , Schappacher Falko M. , Poettgen Rainer , Johrendt Dirk 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Dy2Te3O9: The First Representative of Lanthanoid(III)‐Oxotellurates(IV) with the Composition M2Te3O9.
Authors: Meier Steffen F. , Hoess Patrick , Schleid Thomas 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Mixed Alkaline Earth Aluminides “AIIAl2”.
Authors: Jehle Michael , Wendorff Marco , Roehr Caroline 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Hydrothermal Synthesis and Luminescence Properties of Yb3+ Doped Rare Earth Stannates.
Authors: Jin Dalai , Yu Xiaojing , Yang Hong , Zhu Hongliang , Wang Lina , Zheng Yifan 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Ferroelectric Oxide Made Directly on Silicon.
Authors: Schlom Darrell G. , et al. et al. 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Subsolidus Phase Relations in the System ZnO—B2O3—V2O5.
Authors: Zhan Zhibing , Chen Dagui , Lv Peiwen , Liu Demin , Yan Fengbo , Chen Xianzhi , Huang Feng 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Crystal Structure Refinement and Electronic Properties of Si(cI16).
Authors: Wosylus Aron , Rosner Helge , Schnelle Walter , Schwarz Ulrich 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: LiB6O9F, the First Lithium Fluorooxoborate — Crystal Structure and Ionic Conductivity.
Authors: Cakmak Gueliz , Nuss Juergen , Jansen Martin 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Hexakis(cyanogen)zinc(II) Hexafluoridoarsenate — Synthesis, Structure and a Raman Spectroscopic Study of an Octahedral Homoleptic Cyanogen Complex.
Authors: Beck Johannes , Zink Marcus 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Infinite and Finite Boron Carbon Branched Chains: The Crystal Structures of New Ternary Boride Carbides RE10B7C10 and RE4B3C4.
Authors: Babizhetskyy Volodymyr , Mattausch Hansjuergen , Simon Arndt 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Li(x)Na(2‐x)Mn2S3 and LiNaMnS2 Through Redox‐Induced Ion Exchange Reactions.
Authors: Luthy Joshua A. , Goodman Phillip L. , Martin Benjamin R. 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: An Unexpected Ferromagnetic Coupling in a Dinuclear Manganese(II) Linked Trivacant Heteropolymolybdate Derivative.
Authors: Yang Yanyan , Xu Lin , Gao Guanggang , Li Fengyan , Liu Xizheng , Guo Weihua 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and Crystal Structure of CaCo(H2O)[BP2O8(OH)]·H2O.
Authors: Menezes Prashanth W. , Hoffmann Stefan , Prots Yurii , Kniep Ruediger 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: [Cd3Cu]CuP10, [Cd3Cu] Cluster Stabilized in an Adamantane‐Like Polyphosphide Substructure.
Authors: Bawohl Melanie , Nilges Tom 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis, Structure, and Properties of Ag5Se(PO4) and Ag5Te(PO4) with Two‐Dimensional Framework Trapping PO3‐4 Anions.
Authors: Shestimerova Tatiana A. , Oleneva Olga S. , Mitiaev Alexander S. , Bykov Mikhail A. , Davliatshin Dmitry I. , Shevelkov Andrei V. 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Gold(III) Iminophosphorane Complexes as Catalysts in C—C and C—O Bond Formations.
Authors: Aguilar David , Contel Maria , Navarro Rafael , Soler Tatiana , Urriolabeitia Esteban P. 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Ionic Liquid‐Supported Proline as Catalyst in Direct Asymmetric Aldol Reaction.
Authors: Chen Zhuo , Li Yong , Xie Hui , Hu Chang‐gang , Dong Xian 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Gold‐Catalyzed Hydrofunctionalization of Allenes with Nitrogen and Oxygen Nucleophiles and Its Mechanistic Insight.
Authors: Nishina Naoko , Yamamoto Yoshinori 
Published: June 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.