bluuebunny/crossref_metadata_2025_split huggingface dataset:
Isolation and Culture of Ventral Mesencephalic Precursor Cells and Dopaminergic Neurons from Rodent Brains
Authors: Pruszak Jan , Just Lothar , Isacson Ole , Nikkhah Guido 
Published: December 2009

AbstractThe ability to isolate ventral midbrain (VM) precursor cells and neurons provides a powerful means to characterize their differentiation properties and to study their potential for restoring dopamine (DA) neurons degenerated in Parkinson's disease (PD). Preparation and maintenance of DA VM in primary culture involves a number of critical steps to yield healthy cells and appropriate data. Here, we offer a detailed description of protocols to consistently prepare VM DA cultures from rat and mouse embryonic fetal‐stage midbrain. We also present methods for organotypic culture of midbrain tissue, for differentiation as aggregate cultures, and for adherent culture systems of DA differentiation and maturation, followed by a synopsis of relevant analytical read‐out options. Isolation and culture of rodent VM precursor cells and DA neurons can be exploited for studies of DA lineage development, of neuroprotection, and of cell therapeutic approaches in animal models of PD. Curr. Protoc. Stem Cell Biol. 11:2D.5.1‐2D.5.21. © 2009 by John Wiley & Sons, Inc.
Fluorescence Polarization (FP) Assays for Monitoring Peptide‐Protein or Nucleic Acid‐Protein Binding
Authors: Moerke Nathan J. 
Published: December 2009

AbstractThe technique of fluorescence polarization (FP) is based on the observation that when a fluorescently labeled molecule is excited by polarized light, it emits light with a degree of polarization that is inversely proportional to the rate of molecular rotation. This property of fluorescence can be used to measure the interaction of a small labeled ligand with a larger protein and provides a basis for direct and competition binding assays. FP assays are readily adaptable to a high‐throughput format, have been used successfully in screens directed against a wide range of targets, and are particularly valuable in screening for inhibitors of protein‐protein and protein‐nucleic acid interactions when a small binding epitope can be identified for one of the partners. The protocols in this article describe a general procedure for development of FP assays to monitor binding of such a peptide or oligonucleotide to a protein of interest. Curr. Protoc. Chem Biol. 1:1‐15. © 2009 by John Wiley & Sons, Inc.
Spatiotemporal Dynamics of Kinase Signaling Visualized by Targeted Reporters
Authors: Kunkel Maya T. , Newton Alexandra C. 
Published: December 2009

AbstractThe advent of genetically encoded FRET‐based kinase activity reporters has ushered in a new era of signal transduction research. Such reporters allow the direct monitoring of kinase activity in live cells at specific locations, providing unprecedented information on the spatiotemporal dynamics of kinase signaling. Specifically, FRET‐sensitive conformational changes in the reporters following phosphorylation serve as a direct readout of kinase activity. These genetically encoded reporters allow not only temporal resolution of kinase activity, but also spatial resolution: by fusing appropriate targeting sequences, reporters can be positioned at specific subcellular locations. Herein is presented a strategy to generate and target kinase activity reporters to discrete intracellular regions to measure kinase signaling in live cells. Curr. Protoc. Chem Biol. 1:17‐28. © 2009 by John Wiley & Sons, Inc.
Overview of Liquid Handling Instrumentation for High‐Throughput Screening Applications
Authors: Rudnicki Stewart , Johnston Sean 
Published: December 2009

AbstractLiquid handling in the laboratory has unique challenges specific to the types of research being performed. The devices employed for purposes of performing liquid handling can be broken down into three general categories: bulk reagent dispensers, transfer devices, and plate washers. An overview of these types of liquid handlers, as well as common features and relevance to high‐throughput applications, are discussed in this article. Important topics such as sterility, ease of use, cost, and instrument design advantages and disadvantages are also covered. Curr. Protoc. Chem Biol. 1:43‐54. © 2009 by John Wiley & Sons, Inc.
The law and incomplete database information as confounders in epidemiologic research on occupational injuries and illnesses
Authors: Oleinick Arthur , Zaidman Brian 
Published: December 2009

AbstractBackgroundCapture–recapture studies report undercounting of work injuries/illnesses with days away from work (DAFW) in the Bureau of Labor Statistics annual Survey of Occupational Injuries and Illnesses (BLS SOII) by 25–68% depending on the state and undercounting by various state workers' compensation (WC) systems of eligible claims by 5–35%.MethodsStatutory/regulatory criteria defining eligible cases are used to adjust counts in the 1998–2001 Minnesota's WC system and the BLS SOII to permit comparison and to evaluate the recent studies. Missing information in the employer database used in the capture–recapture studies is tabulated. An attempt is made to harmonize results with two additional databases counting work injuries.ResultsCounts in the BLS SOII moderately undercount by 10–16% the number of WC cases. We believe that matching in capture–recapture studies is adversely affected by misperceptions regarding the application of statutory/regulatory eligibility criteria and by missing data. The result is that the reported undercounts in both the BLS SOII and several state WC databases are overstated in the capture–recapture studies. Although three of four databases can be approximately harmonized, the fourth cannot.ConclusionsMore precisely targeted information is needed before decisions regarding redesign of the BLS survey are made or before legislative or administrative changes in the WC are contemplated. Am. J. Ind. Med. 53:23–36, 2010. © 2009 Wiley‐Liss, Inc.
Researcher judgment and study design: Challenges of using administrative data
Authors: Boden Leslie I. , Ozonoff Al 
Published: December 2009

AbstractBackgroundQuestions have been raised about methods of studies finding substantial undercounting of workplace injuries and illnesses by the Bureau of Labor Statistics (BLS) and workers' compensation agencies. A more recent study of Minnesota concluded that the BLS survey captures 84–90% of workers' compensation cases.MethodsWe examined the sensitivity of findings in two studies to alternate sample definitions and study assumptions.ResultsApplying alternate sample construction rules to the earlier study increased estimated BLS reporting rates from 68% to 77%, assuming source independence. Applying alternate assumptions to the more recent Minnesota study reduced its high estimate of BLS reporting from 90% to 53–64%.ConclusionsStudies linking administrative data from different sources require substantial judgment in constructing research datasets and choosing analytic methods. Moreover, different sample construction rules lead to different results. This suggests that sensitivity analysis should be carried out when alternatives cannot be ruled out. In this case, sensitivity analysis supports the hypothesis of substantial underreporting. Am. J. Ind. Med. 53:37–41, 2010. © 2009 Wiley‐Liss, Inc.
Fluoride catalyzed rearrangements of polysilsesquioxanes, mixed Me, vinyl T8, Me, vinyl T10 and T12 cages
Authors: Ronchi M. , Sulaiman S. , Boston N. R. , Laine R. M. 
Published: December 2009

AbstractInsoluble mixtures of polyvinylsilsesquioxane, ‐(vinylSiO1.5)n‐ PVS, and polymethylsilsesquioxanes, ‐(MeSiO1.5)n‐ PMS, in THF at ambient when treated with catalytic amounts (1–5 mol%) of fluoride ion introduced as tBu4 NF will depolymerize and dissolve. The resulting soluble species consist of [vinylxMe8−x(SiO1.5)]8, [vinylxMe8−x(SiO1.5)]10 and [vinylxMe8−x(SiO1.5)]12. Ratios of 1:1 of PVS:PMS greatly favor formation of vinyl rich cages. Only at ratios of 1:5 are the proportions of vinyl:Me in the cages approximately equal. Of the T8, T10 and T12 species produced, all conditions tried, including changing the solvent to EtOH or toluene or at reflux (THF), favor the formation of the larger cages sometimes completely excluding formation of the T8 materials. Efforts to isolate the cage compounds by removal of solvent regenerates polysilsesquioxanes, albeit those containing mixtures of Me and vinyl groups. Introduction of CaCl2 sufficient to form CaF2 prior to workup prevents repolymerization, allowing recovery of the mixed cage systems. The approach developed here provides a novel way to form mixed functional group silsesquioxane cages. The fact that T10 and T12 cage formation is favored appears to suggest that these cages are more stable than the traditionally produced T8 cages. Copyright © 2009 John Wiley & Sons, Ltd.
Potential impacts of projected sea‐level rise on sea turtle rookeries
Authors: Fuentes MMPB , Limpus CJ , Hamann M , Dawson J 
Published: December 2009

Abstract
Projected sea‐level rise (SLR) is expected to cause shoreline erosion, saline intrusion into the water table and inundation and flooding of beaches and coastal areas. Areas most vulnerable to these physical impacts include small, tropical low‐lying islands, which are often key habitat for threatened and endemic species, such as sea turtles.
Successful conservation of threatened species relies upon the ability of managers to understand current threats and to quantify and mitigate future threats to these species. This study investigated how sea‐level rise might affect key rookeries (nesting grounds) (n=8) for the northern Great Barrier Reef (nGBR) green turtle population, the largest green turtle population in the world.
3‐D elevation models were developed and applied to three SLR scenarios projected by the IPCC 2007 and an additional scenario that incorporates ice melting. Results indicate that up to 38% of available nesting area across all the rookeries may be inundated as a result of SLR.
Flooding, as a result of higher wave run‐up during storms, will increase egg mortality at these rookeries affecting the overall reproductive success of the nGBR green turtle population. Information provided will aid managers to prioritize conservation efforts and to use realistic measures to mitigate potential SLR threats to the nGBR green turtle population. Copyright © 2009 John Wiley & Sons, Ltd.
DNA methylation of cancer genome
Authors: Cheung Hoi‐Hung , Lee Tin‐Lap , Rennert Owen M. , Chan Wai‐Yee 
Published: December 2009

AbstractDNA methylation plays an important role in regulating normal development and carcinogenesis. Current understanding of the biological roles of DNA methylation is limited to its role in the regulation of gene transcription, genomic imprinting, genomic stability, and X chromosome inactivation. In the past 2 decades, a large number of changes have been identified in cancer epigenomes when compared with normals. These alterations fall into two main categories, namely, hypermethylation of tumor suppressor genes and hypomethylation of oncogenes or heterochromatin, respectively. Aberrant methylation of genes controlling the cell cycle, proliferation, apoptosis, metastasis, drug resistance, and intracellular signaling has been identified in multiple cancer types. Recent advancements in whole‐genome analysis of methylome have yielded numerous differentially methylated regions, the functions of which are largely unknown. With the development of high resolution tiling microarrays and high throughput DNA sequencing, more cancer methylomes will be profiled, facilitating the identification of new candidate genes or ncRNAs that are related to oncogenesis, new prognostic markers, and the discovery of new target genes for cancer therapy. Birth Defects Research (Part C) 87:335–350, 2009. Published 2009 by Wiley‐Liss, Inc.
Inherited GPI deficiency: A disorder of histone hypoacetylation
Authors: Georgiou Elisabeth , Layton Mark , Karadimitris Anastasios 
Published: December 2009

AbstractCo‐operative interaction of transcription factors (TF) with epigenetic processes, such as chromatin remodeling and modification (acetylation or methylation), as well as DNA methylation, determine transcriptional activity, activation or repression of a given gene. Mutations disrupting binding of TF to their cognate DNA motifs would be expected to alter the epigenetic landscape of the promoter and selectively affect transcription of the given gene. We review here the transcriptional, epigenetic, biochemical, and clinical consequences of a constitutional mutation in the promoter of PIGM, a housekeeping gene that disrupts binding of the general TF, SP1, thus causing the autosomal recessive disease, inherited glycosylphosphatidylinositol (GPI) deficiency. We suggest that detailed dissection of the function of the mutated PIGM promoter provides important lessons pertinent to the transcriptional and epigenetic control of housekeeping genes as a whole and might have wider therapeutic implications. Birth Defects Research (Part C) 87:327–334, 2009. © 2009 Wiley‐Liss, Inc.
ChemInform Abstract: Structure and Properties of Hydrogenated Ca, Sr, Ba, and Eu Silicides.
Authors: Armbruster Markus , Woerle Michael , Krumeich Frank , Nesper Reinhard 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Influence of Element Substitution on the Cluster Arrangement in the Novel Structures Ca3Tl5, Sr3Tl5, and Sr3Sn5‐xTlx (x = 1.8 and 2.2).
Authors: Kim Sung‐Jin , Faessler Thomas F. 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and Crystal Structures of InGaO3(ZnO)m (m = 2 and 3).
Authors: Keller Isabelle , Assenmacher Wilfried , Schnakenburg Gregor , Mader Werner 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: NH4[BAsO4F] — A New Layer Fluoro‐Boroarsenate with Silicate Related Topology.
Authors: Lieb Alexandra , Weller Mark T. 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The Suboxometallates A9MO4 (A: Rb, Cs; M: Al, Ga, In, Fe, Sc).
Authors: Hoch Constantin , Bender Johannes , Wohlfarth Andreas , Simon Arndt 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The Many Faces of Rare Earth Carbodiimide Compounds.
Authors: Unverfehrt Leonid , Stroebele Markus , Glaser Jochen , Meyer H.‐Juergen 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The Crystal Structures of Er3OFS3 and Er3OF3S2: Two Erbium Oxide Fluoride Sulfides with Condensed Tetrahedral [ZEr4] Units (Z = O and F).
Authors: Strobel Sabine , Mueller Alexander C. , Schleid Thomas 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Ring‐Shaped [(TiCl2)(1,3‐P2S8)]2 Molecules Containing Uncommon [P2S8]2‐ Anions.
Authors: Hoppe Diana , Pfitzner Arno 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis, Crystal Structure, and Magnetic Properties of a Vanadium Oxide Nitride with Pseudobrookite‐Type Structure.
Authors: Nakhal S. , Hermes W. , Ressler T. , Poettgen R. , Lerch M. 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The Effect of Temperature onto the Crystal Structure of Thioantimonates: Solvothermal Syntheses and Crystal Structures of [Co(dien)2] [Co(tren)SbS4]2·0.5H2O and {[Co(tren)]2 [CoSb2S6]}·H2O.
Authors: Lichte Jessica , Luehmann Henning , Naether Christian , Bensch Wolfgang 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Nework Formation by Square‐Planes and Tetrahedra: Polynary Palladiumphosphates MPd2(PO4)2 (M: Ca, Cd, Hg), MPdP2O7 (M: Ca, Sr, Ba, Zn, Hg, Pb), and PbPdSi(P2O7)2.
Authors: Panagiotidis Kosta , Hoffbauer Wilfried , Glaum Robert 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A One‐Dimensional Metal Embedded in Salt Matrices: Synthesis, Modulated Crystal Structures, Electrical Conductivity, and Chemical Bonding of ∞1[PdBi6] [(Bi,Sn)1‐δBr5‐δ′].
Authors: Wahl Bernhard , Kloo Lars , Ruck Michael 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Microwave‐Assisted, Solvent‐Free Oxidative Cleavage of α‐Hydroxyketones.
Authors: Carrera Ignacio , Brovetto Margarita C. , Ramos Juan Carlos , Seoane Gustavo A. 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Asymmetric Construction of Nitrogen‐Containing [2.2.2] Bicyclic Scaffolds Using N‐(p‐Dodecylphenylsulfonyl)‐2‐pyrrolidinecarboxamide.
Authors: Yang Hua , Carter Rich G. 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Structural Diversity Through Gold Catalysis: Stereoselective Synthesis of N‐Hydroxypyrrolines, Dihydroisoxazoles, and Dihydro‐1,2‐oxazines.
Authors: Winter Christian , Krause Norbert 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Cationic Rhodium(I)—dppf Complex‐Catalyzed Olefin Isomerization/Propargyl Claisen Rearrangement/Carbonyl Migration Cascade.
Authors: Tanaka Ken , Okazaki Eri , Shibata Yu 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Stannous Chloride Dihydrate: A Novel and Efficient Catalyst for the Synthesis of gem‐Dihydroperoxides from Ketones and Aldehydes.
Authors: Azarifar Davood , Khosravi Kaveh , Soleimanei Fatemeh 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: TEMPO‐Mediated Oxidation of Primary Alcohols to Carboxylic Acids by Exploitation of Ethers in an Aqueous—Organic Biphase System.
Authors: Mei Zhen‐Wu , Ma Li‐Jian , Kawafuchi Hiroyuki , Okihara Takumi , Inokuchi Tsutomu 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Copper‐Catalyzed Aerobic Oxidative Rearrangement of Tertiary Allylic Alcohols Mediated by TEMPO.
Authors: Vatele Jean‐Michel 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Aerobic Oxidative Iodination of Ketones Catalyzed by Sodium Nitrite “On Water” or in a Micelle‐Based Aqueous System.
Authors: Stavber Gaj , Iskra Jernej , Zupan Marko , Stavber Stojan 
Published: December 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.