bluuebunny/crossref_metadata_2025_split huggingface dataset:
Longitudinal assessment of health‐related quality of life in an observational cohort of patients with cystic fibrosis
Authors: Sawicki Gregory S. , Rasouliyan Lawrence , McMullen Ann H. , Wagener Jeffrey S. , McColley Susanna A. , Pasta David J. , Quittner Alexandra L. 
Published: September 2010

AbstractBackgroundPatient‐reported outcomes (PROs) are increasingly used to evaluate the efficacy of new treatments and the progression of chronic diseases. The Cystic Fibrosis Questionnaire‐Revised (CFQ‐R) is a disease‐specific, PRO measure of health‐related quality of life (HRQOL). We evaluated associations between changes in health status over time and HRQOL in a national CF database.MethodsUsing the Epidemiologic Study of Cystic Fibrosis (ESCF) data, we identified participants who had completed age‐appropriate CFQ‐R assessments on two occasions separated by 9–15 months. Next, we developed multivariate regression models to test whether associations existed between (1) changes in respiratory signs/symptoms and changes in the respiratory health domains of the CFQ‐R, (2) changes in nutritional health status and changes in the nutritional health domains of the CFQ‐R, and (3) changes in treatment complexity and changes in the Treatment Burden scale of the CFQ‐R.ResultsWe analyzed 1,947 pairs of assessments: 337 child (mean age 8.9, range 6–13 years), 581 parent (mean age of child 8.8, range 6–13 years), 398 adolescent (mean age 15.3, range 14–17 years), and 631 adult (mean age 26.9, range 18–73 years). On average, we found little change in both health status indicators and CFQ‐R domain scores over 1 year. Significant associations over time, however, were found between increases in respiratory symptoms and worse CFQ‐R Respiratory Symptom scores, declining weight and worsening scores on CFQ‐R nutritional health domains, and increases in treatment complexity and worsening CFQ‐R Treatment Burden scores for parent respondents.ConclusionHealth status and HRQOL changes were small over a 1‐year period in this CF population. However, changes in respiratory symptoms and weight were associated with significant changes on relevant CFQ‐R scores, indicating that this PRO is sensitive to changes in health status over time. Pediatr Pulmonol. 2011; 46:36–44. © 2010 Wiley‐Liss, Inc.
The relationship between infant lung function and the risk of wheeze in the preschool years
Authors: Pike K.C. , Rose‐Zerilli M.J. , Osvald E. Caffrey , Inskip H.M. , Godfrey K.M. , Crozier S.R. , Roberts G. , Clough J.B. , Holloway J.W. , Lucas J.S. ,   the Southampton Women's Survey Study Group
Published: September 2010

AbstractRationaleThere is evidence that perinatal lung development predicts childhood wheeze. However, very few studies have examined whether preschool wheeze is associated with lower premorbid lung function in early infancy, and as yet there is no information relating atopic and non‐atopic preschool wheeze to early lung development.ObjectiveTo examine the association between premorbid infant lung function and preschool wheeze, and to explore associations with atopic and non‐atopic wheeze phenotypes.MethodsInfant lung function was measured in 147 healthy term infants aged 5–14 weeks. Rapid thoracoabdominal compression was performed during tidal breathing and at raised volume to measure maximal expiratory flow at functional residual capacity $({\rm V'}_{{\rm max}\,{\rm FRC}})$ and forced expiratory volume in 0.4 sec (FEV0.4). Atopic status was determined by skin prick testing at 3 years and wheeze ascertained from parental questionnaires (1 and 3 years).Measurements and Main ResultsLower early infancy ${\rm V'}_{{\rm max}\,{\rm FRC}}$ was associated with wheeze in both the first and third years of life (P = 0.002 and 0.006, respectively). Lower early infancy FEV0.4 was associated with wheeze in the first year (P = 0.03). Compared to non‐atopic children who did not wheeze, non‐atopic children who wheezed in their third year of life had lower FEV0.4 (P = 0.02), while FEV0.4 values of atopic children who wheezed were not significantly different (P = 0.4).ConclusionsLower premorbid infant lung function was present in infants who subsequently wheezed during the first and third years of life. Lower FEV0.4 in early infancy was associated with non‐atopic wheeze but not atopic wheeze at 3 years of age. Pediatr Pulmonol. 2011; 46:75–82. © 2010 Wiley‐Liss, Inc.
Distal mutation modulates the heme sliding in mouse neuroglobin investigated by molecular dynamics simulation
Authors: Xu Jia , Yin Guowei , Du Weihong 
Published: September 2010

AbstractNeuroglobin (Ngb), a hexa‐coordinated hemoprotein primarily expressed in the brain and retina, is thought to be involved in neuroprotection and signal transduction. Ngb can reversibly bind small ligands such as O2 and CO to the heme iron by replacing the distal histidine which is bound to the iron as the endogenous ligand. In this work, molecular dynamics (MD) simulations were performed to investigate the functionally related structural properties and dynamical characteristics in carboxy mouse neuroglobin and three distal mutants including single mutants H64V, K67T and double mutant H64V/K67T. MD simulations suggest that the heme sliding motion induced by the binding of exogenous ligand is affected by the distal mutation obviously. Accompanying changes in loop flexibility and internal cavities imply the structural rearrangement of Ngb. Moreover, the solvent accessibility of heme and some crucial residues are influenced revealing an interactive network on the distal side. The work elucidates that the key residues K67 at E10 and H64 at E7 are significant in modulating the heme sliding and hence the structural and physiological function of Ngb. Proteins 2010. © 2010 Wiley‐Liss, Inc.
Influence of charge distribution on the discrepant MS/MS fragmentation of the native and oxidized FMRF: evidence for the mobile proton model
Authors: Zong Wansong , Liu Rutao , Sun Feng , Zhang Pengjun , Xu Qifei 
Published: September 2010

AbstractUsing the mobile proton model as a framework, the influence of charge distribution on the discrepant fragmentation of peptides FMRF, FM(O)RF and FM(O2)RF (with united peptide sequence) was explored by mass spectrometry experiments and quantum chemical calculations. With the added O atoms, more negative charges were prompted to deposit in the main protonation sites of the oxidation products. Consequently, the solvated proton to the oxidized peptides could flow to the amide bonds in an easier manner and made these bonds fragment easily. Oxidation also induced the discrepant fragmentation of these bonds in a predictable manner: the more negative charges deposited in an amide bond, the more daughter ions (an, bn, yn ions and their derivatives) were produced. The combined methods proposed here refined the mobile proton model for peptide fragmentation and opened the way to probe the discrepant fragmentation of peptides in peptide/protein identification. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd.
Evolution of predictive tools for in situ bioremediation and natural attenuation evaluations
Authors: Burns Matthew , Myers Colleen 
Published: September 2010

AbstractProving the viability of in situ bioremediation technologies and gathering data for its full‐scale implementation typically involves collecting multiple rounds of data and often completing microcosm studies. Collecting these data is cumbersome, time‐consuming, costly, and typically difficult to scale. A new method of completing microcosm studies in situ using an amendable sampling device deployed and incubated in groundwater monitoring wells provides actionable data to expedite site cleanup. The device, referred to as a Bio‐Trap® sampler, is designed to collect actively colonizing microbes and dissolved organic compounds from groundwater for analysis using conventional analytical techniques and advanced diagnostic tools that can answer very specific design and viability questions relating to bioremediation.Key data that can be provided by in situ microcosm studies using Bio‐Trap® samplers include definitively demonstrating contaminant destruction by using compound‐specific isotope analysis and providing data on the mechanism of the degradation by identifying the responsible microbes. Three case studies are presented that demonstrate the combined flexibility of Bio‐Trap® samplers and advanced site diagnostics. The applications include demonstrating natural attenuation of dissolved chlorinated solvents, demonstrating natural attenuation of dissolved petroleum compounds, and using multiple Bio‐Trap® samplers to comparatively assess the viability of bioaugmentation at a chlorinated solvent release site. At each of these sites, the in situ microcosm studies quickly and cost‐effectively answered key design and viability questions, allowing for regulatory approval and successful full‐scale implementation. © 2010 Wiley Periodicals, Inc.
Remediation of DDT‐contaminated soil using optimized mixtures of surfactants and a mixing system
Authors: Ghazali Mirnader , McBean Edward , Shen Hua , Anderson William , Dastous Paul‐André 
Published: September 2010

AbstractSoil contaminated with persistent pesticides, such as DDT, poses a serious risk to humans and to wildlife. A surfactant‐aided soil‐washing technique was studied as an alternative method for remediation of DDT‐contaminated soil. An ex situ soil washing method was investigated using nonionic and anionic surfactants due to the clayey structure of the contaminated soil. A mixture of 1 percent nonionic surfactant (Brij 35) and 1 percent anionic surfactant (SDBS) removed more than 50 percent of DDT from soil in a flow‐through system, whereas individual surfactants or other combinations of the surfactants had a lower removal efficiency. The soil‐washing technique was improved using a mixing system. The mixture of surfactants was optimized in the mixing system, and the combination of 2 percent Brij 35 and 0.1 percent SDBS was found to be optimum, removing 70 to 80 percent of DDT. Prewashing of the soil with tap water decreased the adsorption of surfactants to soil particles by 30 to 40 percent, and postwashing recovered 90 percent of the surfactants. © 2010 Wiley Periodicals, Inc.
Controlling Cell Behavior Through the Design of Polymer Surfaces
Authors: Alves Natália M. , Pashkuleva Iva , Reis Rui L. , Mano João F. 
Published: September 2010

AbstractPolymers have gained a remarkable place in the biomedical field as materials for the fabrication of various devices and for tissue engineering applications. The initial acceptance or rejection of an implantable device is dictated by the crosstalk of the material surface with the bioentities present in the physiological environment. Advances in microfabrication and nanotechnology offer new tools to investigate the complex signaling cascade induced by the components of the extracellular matrix and consequently allow cellular responses to be tailored through the mimicking of some elements of the signaling paths. Patterning methods and selective chemical modification schemes at different length scales can provide biocompatible surfaces that control cellular interactions on the micrometer and sub‐micrometer scales on which cells are organized. In this review, the potential of chemically and topographically structured micro‐ and nanopolymer surfaces are discussed in hopes of a better understanding of cell–biomaterial interactions, including the recent use of biomimetic approaches or stimuli‐responsive macromolecules. Additionally, the focus will be on how the knowledge obtained using these surfaces can be incorporated to design biocompatible materials for various biomedical applications, such as tissue engineering, implants, cell‐based biosensors, diagnostic systems, and basic cell biology. The review focusses on the research carried out during the last decade.
A novel triple‐wavelength colorimetric method for measuring amylose and amylopectin contents
Authors: Wang JinPeng , Li Yin , Tian YaoQi , Xu XueMing , Ji XueXia , Cao Xu , Jin ZhengYu 
Published: September 2010

AbstractA novel triple‐wavelength colorimetry based on the principle of iodine binding was developed to determine amylose and amylopectin contents. Detection wavelengths of different starch material were different for each method. Comparative trials were carried out by both single‐ and dual‐wavelength colorimetry. We found that single‐wavelength colorimetry could only be used to evaluate amylose content. Correlation coefficient of standard curves for amylopectin content determination was improved by increase of detection wavelength numbers. The precision and recovery coefficient was also improved by triple‐wavelength colorimetry compared to single‐ and dual‐wavelength colorimetry. Percent amylose of cassava, potato, and high‐amylose maize determined by triple‐wavelength were 19.3, 21.8, and 42.3%, respectively, and percent amylopectin were 78.9, 76.9, and 54.5%, respectively. This developed triple‐wavelength colorimetry could provide accurate data for evaluating amylose content and have potential for amylopectin content detection.
Convergent Genesis of an Adult Neural Crest-Like Dermal Stem Cell from Distinct Developmental Origins
Authors: Jinno Hiroyuki , Morozova Olena , Jones Karen L. , Biernaskie Jeffrey A. , Paris Maryline , Hosokawa Ryoichi , Rudnicki Michael A. , Chai Yang , Rossi Fabio , Marra Marco A. , Miller Freda D. 
Published: September 2010

Abstract
Skin-derived precursors (SKPs) are multipotent dermal stem cells that reside within a hair follicle niche and that share properties with embryonic neural crest precursors. Here, we have asked whether SKPs and their endogenous dermal precursors originate from the neural crest or whether, like the dermis itself, they originate from multiple developmental origins. To do this, we used two different mouse Cre lines that allow us to perform lineage tracing: Wnt1-cre, which targets cells deriving from the neural crest, and Myf5-cre, which targets cells of a somite origin. By crossing these Cre lines to reporter mice, we show that the endogenous follicle-associated dermal precursors in the face derive from the neural crest, and those in the dorsal trunk derive from the somites, as do the SKPs they generate. Despite these different developmental origins, SKPs from these two locations are functionally similar, even with regard to their ability to differentiate into Schwann cells, a cell type only thought to be generated from the neural crest. Analysis of global gene expression using microarrays confirmed that facial and dorsal SKPs exhibit a very high degree of similarity, and that they are also very similar to SKPs derived from ventral dermis, which has a lateral plate origin. However, these developmentally distinct SKPs also retain differential expression of a small number of genes that reflect their developmental origins. Thus, an adult neural crest-like dermal precursor can be generated from a non-neural crest origin, a finding with broad implications for the many neuroendocrine cells in the body.
Catalytic carboborations
Authors: Suginome Michinori 
Published: September 2010

AbstractTransition‐metal‐catalyzed carboborations, in which organic and boryl groups are introduced concomitantly to unsaturated organic molecules, have been developed. Direct carboborations, which proceed via activation of B–C bonds of cyanoboranes and alkynylboranes, and transmetalative carboborations, in which activation of B–Cl bonds is followed by transmetalation from organotin and organozirconium reagents, are overviewed. In addition, as representative results and mechanistic considerations, the scientific background and a personal view behind the study are also described. © 2010 The Japan Chemical Journal Forum and Wiley Periodicals, Inc. Published online in Wiley InterScience (www.interscience.wiley.com)
Development of selective inhibitors of necrosis
Authors: Sodeoka Mikiko , Dodo Kosuke 
Published: September 2010

AbstractOxidative stress‐induced necrosis plays an important role in ischemia‐reperfusion injury, such as stroke and heart attack. Here, we describe the development of selective inhibitors of necrosis, MS‐1 and IM‐54, as potential cardioprotective agents and biological tools for investigating the molecular mechanisms of cell death. By means of chemical modifications of kinase inhibitor BM I, its affinity for various kinases was successfully removed and a potent and selective inhibitor of necrosis, IM‐54, was obtained. IM‐54 inhibits necrosis induced by oxidative stress, but not apoptosis induced by anticancer drugs. © 2010 The Japan Chemical Journal Forum and Wiley Periodicals, Inc. Published online in Wiley InterScience (www.interscience.wiley.com) DOI 10.1002/tcr.201000031
A new yeast genetic resource for analysis and breeding
Authors: Timberlake William E. , Frizzell Margaret A. , Richards Keith D. , Gardner Richard C. 
Published: September 2010

AbstractWe made a library of Saccharomyces cerevisiae F1 hybrids from all possible crosses of 16 wild‐type strains, including two common laboratory strains and two commercial winemaking varieties. Fourteen of the starting strains have been sequenced. Thus, the sequences of both genomes are known in 182 novel hybrids, and the sequence of one genome is known in 56. All tested strains sporulated. Fertilities were in the range 0–100%. Hybrids showed no more variation than parental strains for ethanol production, ethanol tolerance or growth at temperature extremes, but some F1s appeared to display hybrid vigour (heterosis). We tested four tetrads from one hybrid for their ability to grow at low temperature or in the presence of an inhibitory concentration of ethanol. Only one F2 was as tolerant as the most tolerant F0 parent. A few showed intermediate tolerance, but most were less tolerant than either parent or the F1 hybrid, consistent with uncoupling of genes contributing to an optimized quantitative trait. The diversity and structure of the library should make it useful for analysis of genetic interactions among diverse strains, quantitative inheritance and heterosis, and for breeding. Copyright © 2010 John Wiley & Sons, Ltd.
Farming with native bees (Apis mellifera subsp. capensis Esch.) has varied effects on nectar‐feeding bird communities in South African fynbos vegetation
Authors: Geerts Sjirk , Pauw Anton 
Published: September 2010

AbstractOutside their natural range, honeybees (Apis mellifera) are known to have detrimental effects on indigenous pollinators through exploitative or interference competition, but little is known about the effect of honeybee farming in areas where honeybees occur naturally. In the Cape Floristic Region of South Africa, where honeybees are indigenous, managed hives potentially elevate the abundance of honeybees far above natural levels, but impacts on other floral resource‐dependent species have not been studied. Here we use experimental manipulation of honeybee density to test whether honeybee farming affects nectar‐feeding birds. We selected the common sugarbush (Protea repens), utilized by both birds and bees, and analysed the time (before/after) by treatment (control/experiment) interaction to explore changes in bee abundance, nectar availability and bird abundance at three sites. Hive addition increased honeybee abundance in inflorescences of P. repens above expected levels. Despite experimental increase in honeybee numbers, there is no reduction in nectar sugar availability relative to the control areas. Where honeybee density was highest, sugarbird (Promerops cafer) numbers declined relative to expected, but sunbirds (Nectarinidae) were not affected at any of the sites. We conclude that stocking rates of more than one honey bee per P. repens inflorescence have detrimental effects on bird abundance due to interference, rather than resource competition.
Elucidation of Phosphatidylcholine Composition in Krill Oil Extracted from Euphausia superba
Authors: Winther Bjørn , Hoem Nils , Berge Kjetil , Reubsaet Léon 
Published: September 2010

AbstractHigh performance liquid chromatography‐electrospray tandem mass spectrometry was used to elucidate the phospholipids in krill oil extracted from Euphausia superba, an emerging source for human nutritional supplements. The study was carried out in order to map the species of the choline‐containing phospholipid classes: phosphatidylcholine and lyso‐phosphatidylcholine. In addition, the prevalent phosphatidylcholine class was quantified and the results compared with prior analysis. The qualification was performed with separation on a reverse phase chromatography column, while the quantification was obtained with class separation on a normal phase chromatography column. An Orbitrap system was used for the detection, and pulsed‐Q dissociation fragmentation was utilized for the identification of the species. An asymmetrical exclusion list was applied for detection of phospholipid species of lower concentration, significantly improving the number of species observed. A total of 69 choline‐containing phospholipids were detected, whereof 60 phosphatidylcholine substances, among others seven with probable omega‐3 fatty acids in both sn‐1 and sn‐2. The phosphatidylcholine concentration was estimated to be 34 ± 5 g/100 g oil (n = 5). These results confirm the complexity of the phospholipid composition of krill oil, and the presence of long chained, heavily unsaturated fatty acids.
Changes in Lipid Composition During Dry Grind Ethanol Processing of Corn
Authors: Moreau Robert A. , Liu Keshun , Winkler‐Moser Jill K. , Singh Vijay 
Published: September 2010

AbstractDuring the dry grind ethanol process, ground corn is fermented and the major co‐product is a feed called distillers dried grains with solubles (DDGS). This study investigated the changes that occur in the composition of corn oil that can be extracted from various process fractions during the dry grind ethanol process. In the first part of this study, samples of distillers dried grains, thin stillage, condensed distillers solubles (also known as syrup), and DDGS were obtained from 7 dry grind ethanol plants. The levels of deleterious free fatty acids were high (>7%) and those of valuable total phytosterols were also high in all fractions (>2%). In the second part of this study, changes in the content and composition of the fatty acids, phytosterols, tocopherols and tocotrienols were quantitatively analyzed in crude oil samples extracted from nine dry grind process fractions from three commercial ethanol plants. Fatty acid and phytosterol composition remained nearly constant in all nine fractions, although some significant variations in phytosterol composition existed among the fractions. Examination of the tocopherols and tocotrienols revealed that γ‐tocopherol was the most abundant tocol in ground corn but an unknown tocol became the predominant tocol after fermentation and persisted in the remaining processing fractions and in the final DDGS product. Overall, the remaining majority of tocols remained relatively unchanged.