bluuebunny/crossref_metadata_2025_split huggingface dataset:
A vocational education and training subject teacher for fostering students' entrepreneurial skills—The development of a conceptual model
Authors: Kolho Piia 
Published: June 2024

AbstractIn the future, a significant portion of the workforce is expected to engage in self‐employment or pursue part‐time entrepreneurial endeavours, especially in vocational education and training (VET) fields where entrepreneurship serves as a predominant avenue of employment. The primary goal of this article is to investigate the interconnectedness of a teacher's autonomy, agency, and guiding behaviour and to understand their relevance to the teacher's pedagogical decisions and ability to effectively guide students toward entrepreneurship. To achieve this objective, semistructured interviews were conducted with eight VET subject teachers, employing predetermined concept definitions derived from prior research. Analysis of the interviews revealed a connection between these concepts, highlighting their influence on the pedagogical decisions made by VET teachers. However, minor variations were observed across diverse fields of VET. The study underscores the significance of autonomy, decision‐making capabilities and teacher agency in fostering students' entrepreneurial skills. It emphasises that autonomy alone is insufficient, and various factors either facilitate or hinder the promotion of entrepreneurship. This article contributes to entrepreneurship education research in two ways. First, it provides new information about the VET subject teacher as a promoter of students' entrepreneurial skills. Second, by presenting a theoretical model that helps to understand the factors influencing teachers' actions. The model, being novel, can be utilised to understand how VET teachers utilise autonomy and express agency in their work. Additionally, the research results are considered from an international perspective. The discourse revolves around the conceptual model, by exploring the reasons behind it, and considering subject teachers' professional identity as entrepreneurship teachers.
Early warning tools and clinician ‘agency’ for strengthening safety culture: An integrative review
Authors: Ferguson Bridget , Baldwin Adele , Henderson Amanda 
Published: June 2024

AbstractAimIdentify and analyse literature investigating nurses' and midwives' use of early warning tools during the care of adult inpatients.DesignAn integrative literature review.MethodsWhittemore and Knafl's (2005) framework guided this integrative review. PubMed, CINAHL, EMCARE and Google Scholar were systematically searched. The authors assessed the methodological quality of 21 papers meeting inclusion criteria and thematically analysed key data.ResultsThree main themes were identified, each with further sub‐themes.ConclusionEarly warning tools operate within various systems and cultural contexts. However, their potential for improved patient safety may be hindered. Protocols influencing tool usage may make nurses and midwives distanced from patients and their expertise. For early warning tools to enhance patient safety, assessing their integration into practice is crucial to maximizing effectiveness.ImpactThis review emphasizes the importance of integrating human relationships with early warning tools for patient safety.Patient or Public ContributionThis integrative literature review does not include patient or public input.Implications for Practice/PolicyAdapting early warning tools to balance standardization for safety and efficiency and promoting nurses' and midwives' expertise and autonomy is required to optimize delivery of quality care and uphold patient safety.Reporting MethodThe Preferred Reporting Items for Systematic Reviews and Meta‐analyses guidelines were used.
Whole‐exome sequencing for genetic diagnosis of idiopathic liver injury in children
Authors: Lülecioğlu Aysima Atılgan , Yazıcı Yılmaz Yücehan , Baran Alperen , Warasnhe Khaled , Beyaz Şengül , Aytekin Caner , Özçay Figen , Aydemir Yusuf , Barış Zeren , Belkaya Serkan 
Published: June 2024

AbstractGenome‐wide approaches, such as whole‐exome sequencing (WES), are widely used to decipher the genetic mechanisms underlying inter‐individual variability in disease susceptibility. We aimed to dissect inborn monogenic determinants of idiopathic liver injury in otherwise healthy children. We thus performed WES for 20 patients presented with paediatric‐onset recurrent elevated transaminases (rELT) or acute liver failure (ALF) of unknown aetiology. A stringent variant screening was undertaken on a manually‐curated panel of 380 genes predisposing to inherited human diseases with hepatobiliary involvement in the OMIM database. We identified rare nonsynonymous variants in nine genes in six patients (five rELT and one ALF). We next performed a case‐level evaluation to assess the causal concordance between the gene mutated and clinical symptoms of the affected patient. A genetic diagnosis was confirmed in four rELT patients (40%), among whom two carried novel mutations in ACOX2 or PYGL, and two had previously‐reported morbid variants in ABCB4 or PHKA2. We also detected rare variants with uncertain clinical significance in CDAN1, JAG1, PCK2, SLC27A5 or VPS33B in rELT or ALF patients. In conclusion, implementation of WES improves diagnostic yield and enables precision management in paediatric cases of liver injury with unknown aetiology, in particular recurrent hypertransaminasemia.
Effect of monosodium glutamate supplementation in diet on fatty acid profiles, genes expression related to lipid metabolism and egg yolk cholesterol in late phase of production in laying hens
Authors: Kazemifard Mohammad , Rezaei Mansour , Kardel Ali Asghar , Pirsaraei Zarbakht Ansari , Dirandeh Essa 
Published: June 2024

AbstractIn an experiment with four treatments and five replicates, the effects of adding monosodium glutamate (MSG) to the diet in late phase of egg production was studied on performance, and lipid metabolism in laying hens. Dietary treatments included the control basal diet without MSG and the other treatments adding 0.4%, 0.8% and 1.2% MSG in the control diet respectively. The effect of supplementation of MSG on egg weight, egg production, feed conversion ratio and egg mass was insignificant (p < 0.05). Adding MSG to the diet significantly increased feed intake and blood polyunsaturated fatty acids concentration (p < 0.05). Intake of 0.8% and 1.2% MSG in the diet up regulated the mRNA expression of acetyl‐coenzyme A carboxylase, fatty acid synthase and lipoprotein lipase in the abdominal and liver tissues in comparison to the control group. Blood very low‐density lipoprotein‐cholesterol, triglycerides and cholesterol concentration were increased in treatment fed with a diet containing 0.8% MSG compared to the control group (p < 0.05). The effect of MSG on total egg yolk cholesterol concentration was not significant. In conclusion, the results of the present experiment indicated that adding MSG increased feed intake and blood polyunsaturated fatty acid concentration.
Deciphering the mechanism of E3 ubiquitin ligases in plant responses to abiotic and biotic stresses and perspectives on PROTACs for crop resistance
Authors: Su Yingying , Ngea Guillaume Legrand Ngolong , Wang Kaili , Lu Yuchun , Godana Esa Abiso , Ackah Michael , Yang Qiya , Zhang Hongyin 
Published: June 2024

SummaryWith global climate change, it is essential to find strategies to make crops more resistant to different stresses and guarantee food security worldwide. E3 ubiquitin ligases are critical regulatory elements that are gaining importance due to their role in selecting proteins for degradation in the ubiquitin‐proteasome proteolysis pathway. The role of E3 Ub ligases has been demonstrated in numerous cellular processes in plants responding to biotic and abiotic stresses. E3 Ub ligases are considered a class of proteins that are difficult to control by conventional inhibitors, as they lack a standard active site with pocket, and their biological activity is mainly due to protein–protein interactions with transient conformational changes. Proteolysis‐targeted chimeras (PROTACs) are a new class of heterobifunctional molecules that have emerged in recent years as relevant alternatives for incurable human diseases like cancer because they can target recalcitrant proteins for destruction. PROTACs interact with the ubiquitin‐proteasome system, principally the E3 Ub ligase in the cell, and facilitate proteasome turnover of the proteins of interest. PROTAC strategies harness the essential functions of E3 Ub ligases for proteasomal degradation of proteins involved in dysfunction. This review examines critical advances in E3 Ub ligase research in plant responses to biotic and abiotic stresses. It highlights how PROTACs can be applied to target proteins involved in plant stress response to mitigate pathogenic agents and environmental adversities.
Utilising soybean [Glycine max (L.) Merr.] wild and untapped genetic resources through pre‐breeding: Challenges and opportunities
Authors: Bhartiya Anuradha , Aditya Jay prakash , Gupta Sanjay , Rajesh Vangla , Nataraj Vennampally , Kant Lakshmi , Joshi Hemlata 
Published: June 2024

AbstractSoybean is an economically important oilseed crop with diverse uses as food, feed, biofuel and so forth. Although the demand for soybean is growing rapidly worldwide, the narrow genetic base of released cultivars due to meagre utilisation of available wild and untapped genetic resources is a major factor contributing to stagnant production and productivity levels of soybean. Considering the continuously increasing global population, changing dietary habits, ongoing climatic fluctuations, rapidly evolving biotic stresses and shrinking land and water resources, utilisation of novel and diverse sources of variation is required for the genetic enhancement of soybean. To widen the genetic base of commercial soybean cultivars and to improve the genetic gain of the crop focused and systematic pre‐breeding efforts can play a game‐changing role by supplying novel genes in crop improvement programs. This article discusses the rationale of tapping wild and untapped genetic resources of soybean, gene pools, soybean genetic resources conserved ex‐situ and its utilisation status, genetic base of commercial soybean cultivars, identified novel genes and genomic regions in wild genetic resources, pre‐breeding efforts in India and strategies for incorporating desirable traits in the elite cultivated gene pool for fulfilling the requirements of farmers and other end‐users.
Prophylactic sildenafil to prevent bronchopulmonary dysplasia: A systematic review and meta‐analysis
Authors: Hirata Katsuya , Nakahari Atsuko , Takeoka Mami , Watanabe Masahiko , Nishimura Yutaka , Katayama Yoshinori , Isayama Tetsuya ,   Japan Evidence Based Neonatology (JEBNeo)
Published: June 2024

AbstractBackgroundBronchopulmonary dysplasia (BPD) persists as one of the foremost factors contributing to mortality and morbidity in extremely preterm infants. The effectiveness of administering sildenafil early on to prevent BPD remains uncertain. The aim of this study was to investigate the efficacy and safety of prophylactically administered sildenafil during the early life stages of preterm infants to prevent mortality and BPD.MethodsMEDLINE, Embase, Cochrane Central Register of Controlled Trials, Cumulative Index to Nursing and Allied Health Literature, and Ichushi were searched. Published randomized controlled trials (RCTs), non‐RCTs, interrupted time series, cohort studies, case–control studies, and controlled before‐and‐after studies were included. Two reviewers independently screened the title, abstract, and full text, extracted data, assessed the risk of bias, and evaluated the certainty of evidence (CoE) following the Grading of Recommendations Assessment and Development and Evaluation approach. The random‐effects model was used for a meta‐analysis of RCTs.ResultsThis review included three RCTs (162 infants). There were no significant differences between the prophylactic sildenafil and placebo groups in mortality (risk ratio [RR]: 1.32; 95% confidence interval [CI]: 0.16–10.75; very low CoE), BPD (RR: 1.20; 95% CI: 0.79–1.83; very low CoE), and all other outcome assessed (all with very low CoE). The sample sizes were less than the optimal sizes for all outcomes assessed, indicating the need for further trials.ConclusionsThe prophylactic use of sildenafil in individuals at risk of BPD did not indicate any advantageous effects in terms of mortality, BPD, and other outcomes, or increased side effects.
Early modern women philosophers and politics: Accommodating sphere restrictions
Authors: Bergès Sandrine 
Published: June 2024

AbstractIn his Politics, Aristotle decreed that human beings needed to take part in politics to flourish, but that women, despite being human, needed to stay at home and away from politics. This paper offers an overview of how early modern women philosophers worked to makes their lives more political despite being constricted to the domestic sphere. Lucrezia Marinella argued that the home was like a small city, requiring quasi political skill to run, Cavendish believed that politics should cover the home and its inhabitants, not just the so‐called ‘public domain’. Mary Astell, less optimistic, thought that women could compensate for the urge to be political by retreating into a well‐educated mind.
Impact of papain on the treatment of raw diluted dromedary semen
Authors: Abdel‐Ghani Mohammed A. , Ghoneim Ibrahim M. , Nagano Masashi , AlMomen Hussain Qasem M. 
Published: June 2024

AbstractA variety of parameters, including liquefaction and semen viscosity, affect the sperm's ability to travel and reach the egg for fertilization and conception. Given that the details behind the viscosity of the semen in male camels have not yet been fully clarified, the purpose of this study was to ascertain how the addition of papain affected the viscosity of fresh diluted camel semen. The study examined semen samples derived from camels that had distinct viscosities. Sperm motility, viability, abnormal sperm percentage, concentration, viscosity, morphometry, acrosome integrity and liquefaction were among the evaluations following 0, 5, 10, 20 or 30 min of incubation at 37°C with papain (0.004 mg/mL, 0.04 mg/mL or 0.4 mg/mL; a semen sample without papain was used as a control). A statistically significant interaction between the effects of papain concentrations and incubation time was found (F = 41.68, p = .0001). Papain concentrations (p = .0001) and incubation times (p = .0001) both had a statistically significant impact on viscosity, according to a simple main effects analysis. A lower viscosity was found (p < .05) at 0.04 mg/mL (0.1 ± 0.0) after 10 min of incubation. A simple main effects analysis showed that papain concentrations and incubation time have a statistically significant effect on sperm motility (p = .0001). At 0.04 mg/mL papain, the sperm motility % was higher (p < .05) after 10 min (64.4 ± 4.8), 20 min (68.4 ± 6.2), and 30 min incubation (72.2 ± 6.6) compared to 0, 5 min (38.3 ± 4.1 and 51.6 ± 5.0, respectively). In conclusion, the fresh diluted camel semen had the lowest viscosity properties after 10 min of incubation with 0.04 mg/mL papain, without compromising sperm motility, viability, acrosome integrity and sperm morphology.
A Framework for Developing Systematic Testbeds for Multifidelity Optimization Techniques
Authors: Tao Siyu , Sharma Chaitra , Devanathan Srikanth 
Published: June 2024

Abstract
Multifidelity (MF) models abound in simulation-based engineering. Many MF strategies have been proposed to improve the efficiency in engineering processes, especially in design optimization. When it comes to assessing the performance of MF optimization techniques, existing practice usually relies on test cases involving contrived MF models of seemingly random math functions, due to limited access to real-world MF models. While it is acceptable to use contrived MF models, these models are often manually written up rather than created in a systematic manner. This gives rise to the potential pitfall that the test MF models may be not representative of general scenarios. We propose a framework to generate test MF models systematically and characterize MF optimization techniques' performances comprehensively. In our framework, the MF models are generated based on given high-fidelity (HF) models and come with two parameters to control their fidelity levels and allow model randomization. In our testing process, MF case problems are systematically formulated using our model creation method. Running the given MF optimization technique on these problems produces what we call “savings curves” that characterize the technique's performance similarly to how receiver operating characteristic (ROC) curves characterize machine learning classifiers. Our test results also allow plotting “optimality curves” that serve similar functionality to savings curves in certain types of problems. The flexibility of our MF model creation facilitates the development of testing processes for general MF problem scenarios, and our framework can be easily extended to other MF applications than optimization.
Structural–Parametric Synthesis of Single-Loop 6R and 7R Mechanisms Using Factorization of Motion Polynomials and Its Application in Grippers
Authors: Li Meng , Liu Kai , Liu Jingfang , Ding Huafeng 
Published: June 2024

Abstract
In this study, the so-called quasi-spiral motion pattern of a particular 3R (revolute) chain, which could track a quasi-spiral curve, is discovered for application in enveloping gripping. Naturally, a novel perspective on the synthesis of 1-DOF (degree of freedom) single-loop 6R and 7R mechanisms is presented to implement the quasi-spiral motion pattern. In these processes, the method of factorization of the motion polynomials is used to synthesize a series of single-loop mechanisms with the motion to generate quasi-spiral curves. By employing the proposed method, numerous innovative 6R and 7R mechanisms have been developed to construct dual layers of grippers based on specific network regulations. Taking a novel 6R mechanism as an example, the forward solution of the mechanism is determined, and the relationship between different rotation angles is obtained. Then, the changes in each angle are displayed. Finally, the novel 6R and 7R mechanisms are employed as units to determine the networking method and assemble new types of grippers. Grippers formed by the aforementioned 1-DOF mechanisms exhibit the characteristics of enveloping grasping.
Effects of Brief Mindfulness Training on Basketball Free-Throw Shooting Performance Under Pressure: A Randomized Controlled Trial of Dosage Response
Authors: Arthur-Cameselle Jessyca N. , Keeler Linda A. 
Published: June 2024

Studies have indicated that as little as 15 min of mindfulness training (MT) positively affects sport performance under pressure, but the minimum amount of MT required to induce effects is unclear. The current experiment tested the effects of MT of different lengths on free-throw shooting under pressure. Forty-six participants (78% men) with competitive basketball experience completed pretest mindfulness and anxiety surveys and shot under low pressure. Using performance-based matched assignment, participants were randomly distributed into groups. On another day, participants completed audio trainings (6-min MT, 15-min MT, or control) and then shot under high pressure. Under high pressure, anxiety and mindfulness states did not differ among groups, nor were there group differences in average shooting percentage. However, only the control group performed worse on the second shot under high pressure compared with low pressure, suggesting possible protection effects of MT. Findings are discussed regarding application and possible interactions between traits, motivation, and incentive values.
Identification of genetic markers of resistance to macrolide class antibiotics in
Mannheimia haemolytica
isolates from a Saskatchewan feedlot
Authors: Deschner Darien , Voordouw Maarten J. , Fernando Champika , Campbell John , Waldner Cheryl L. , Hill Janet E. 
Published: June 2024

ABSTRACT

Mannheimia haemolytica
is a major contributor to bovine respiratory disease (BRD), which causes substantial economic losses to the beef industry, and there is an urgent need for rapid and accurate diagnostic tests to provide evidence for treatment decisions and support antimicrobial stewardship. Diagnostic sequencing can provide information about antimicrobial resistance genes in
M. haemolytica
more rapidly than conventional diagnostics. Realizing the full potential of diagnostic sequencing requires a comprehensive understanding of the genetic markers of antimicrobial resistance. We identified genetic markers of resistance in
M. haemolytica
to macrolide class antibiotics commonly used for control of BRD. Genome sequences were determined for 99
M. haemolytica
isolates with six different susceptibility phenotypes collected over 2 years from a feedlot in Saskatchewan, Canada. Known macrolide resistance genes
estT
,
msr
(E), and
mph
(E) were identified in most resistant isolates within predicted integrative and conjugative elements (ICEs). ICE sequences lacking antibiotic resistance genes were detected in 10 of 47 susceptible isolates. No resistance-associated polymorphisms were detected in ribosomal RNA genes, although previously unreported mutations in the L22 and L23 ribosomal proteins were identified in 12 and 27 resistant isolates, respectively. Pangenome analysis led to the identification of 79 genes associated with resistance to gamithromycin, of which 95% (75 of 79) had no functional annotation. Most of the observed phenotypic resistance was explained by previously identified antibiotic resistance genes, although resistance to the macrolides gamithromycin and tulathromycin was not explained in 39 of 47 isolates, demonstrating the need for continued surveillance for novel determinants of macrolide resistance.

IMPORTANCE

Bovine respiratory disease is the costliest disease of beef cattle in North America and the most common reason for injectable antibiotic use in beef cattle. Metagenomic sequencing offers the potential to make economically significant reductions in turnaround time for diagnostic information for evidence-based selection of antibiotics for use in the feedlot. The success of diagnostic sequencing depends on a comprehensive catalog of antimicrobial resistance genes and other genome features associated with reduced susceptibility. We analyzed the genome sequences of isolates of
Mannheimia haemolytica
, a major bovine respiratory disease pathogen, and identified both previously known and novel genes associated with reduced susceptibility to macrolide class antimicrobials. These findings reinforce the need for ongoing surveillance for markers of antimicrobial resistance to support improved diagnostics and antimicrobial stewardship.