P3-S1.07 Chlamydia trachomatisserovar distribution and other sexually transmitted coinfections in subjects attending a STD outpatients clinic in Italy
Authors: Marangoni A , Donati M , D'Antuono A , Di Francesco A , Ostanello F , Foschi C , Nardini P , Banzola N , Cevenini R 
Published: July 2011

BackgroundChlamydia trachomatisis the leading cause of bacterial sexually transmitted diseases (STDs) in industrialised countries. omp1 (ompA), the gene encoding the major outer membrane protein (MOMP), has been widely used for molecular epidemiology, because it contains four spaced variable domains.MethodsA total of 1625 patients attending the STD Outpatients Clinic of St. Orsola University Hospital of Bologna, Italy were enrolled for this study. Each patient was clinically visited, bled in order to perform serological tests, than three urethral or endocervical swabs were obtained. Two swabs were cultured for the detection ofC trachomatisandNeisseria gonorrhoeae, whereas the third was stored at −80°C. When a positive result was obtained byC trachomatisculture, the corresponding frozen sample was withdrawn, its DNA was extracted by VERSANT kPCR SP Module (Siemens Healthcare Diagnostics Inc.) and used as a template for omp1 gene fragment amplification. PCR products were purified and both strands were sequenced. Nucleotide sequences were compared to omp1 sequences using the BLAST search tool at the National Center for Biotechnology Information. The sequences were manually aligned using BioEdit (version 7.0.0) software. χ2Test was used and a p value of <0.05 was considered statistically significant.ResultsC trachomatiswas detected in 103 out of 1625 (6.3%) swabs by culture. Prevalence was significantly higher in men (p<0.01), with 60 positives out of 525 tested (11.4%), than in women (43/1100; 3.9%), as well as presence of clinical symptoms: 81.7% (49/60) of infected men and 44.2% of infected women (19/43) were symptomatic. Also prevalence of STD coinfections was significantly higher (p<0.01) in men (35/60; 58.3%) than in women (8/43; 18.6%). In our population the most common serovar was E, with a prevalence of 38.8%, followed by G (23.3%), F (13.5%), D/Da (11.6%), and J (4.8%). Statistically significant differences (p=0.042) in serovar prevalence between men and women were detected. Finally, significant differences (p=0.035) were detected when serovar distribution among patients with or without coinfection was studied: patients with an infection due to D/Da had the highest coinfection rate (75.0%), whereas coinfection rates among patients with serovars F, E, and G were 57.1%, 37.5%, and 29.2%, respectively see Abstract P3-S1.07 table 1.Abstract P3-S1.07 Table 1Primary demographic, epidemiological, and clinical data and rates of infection withC trachomatisserovars for male and female patientsSex No (%) of patientsMalesFemalep Value (χ2test)Place of birth Italy42 (70.0)22 (51.2)0.052 Other18 (30.0)21 (48.8)Symptoms Yes49 (81.7)19 (44.2)0.000* No11 (18.3)24 (55.8)N gonorrhoeaecoinfection Yes27 (45.0)3 (7.0)0.000* No33 (55.0)40 (93.0)T pallidumcoinfection Yes5 (8.3)2 (4.7)0.696 No55 (91.7)41 (95.3)Human papillomavirus coinfection Yes6 (10.0)3 (7.0)0.592 No54 (90.0)40 (93.0)HIV coinfection Yes5 (8.3)0 (0,0)0.073 No55 (91.7)43 (100)C trachomatisserovar B0 (0,0)2 (4.7)0.042* D/Da10 (16.7)2 (4.7) E24 (40.0)16 (37.2) F11 (18.3)3 (7.0) G10 (16.7)14 (32.6) H0 (0.0)2 (4.7) I/Ia2 (3.3)0 (0.0) J2 (3.3)3 (7.0) K1 (1.7)1 (2.3)*Statistically significant (p<0.01).ConclusionsThe present study contributed to increase the knowledge on serovar distribution ofC trachomatisin Italy.