bluuebunny/crossref_metadata_2025_split huggingface dataset:
Skin reactions induced by experimental exposure to setae from larvae of the northern pine processionary moth (Thaumetopoea pinivora)
Authors: Fagrell Bengt , Jörneskog Gun , Salomonsson Ann‐Christin , Larsson Stig , Holm Go¨ran 
Published: August 2010

Background:  Moth larvae that carry noxious hairs (setae) are spread worldwide. A population of the northern pine processionary moth (Thaumetopoea pinivora, TP) is present on the island of Gotland in the Baltic Sea. This study aimed to evaluate the local skin reactions following experimental exposure to TP setae.Subjects and Methods:  A drop of setae suspension was applied on the volar forearm of six volunteers. The local skin reactions were studied by microscopy and skin perfusion using laser Doppler (LD) scanning.Results:  Setae penetrated into the skin, and LD scanning showed marked increase in blood perfusion in all subjects. In two, with a history of severe symptoms, microscopic vacuoles developed around setae, followed by desquamation and severe symptoms. Remaining individuals, with only light symptoms during previous exposure, exhibited only mild reactions that disappeared within 3 weeks. In none of the volunteers, immunoglobulin (Ig) E or IgG4 antibodies to larval antigens were found.Conclusion:  Experimental skin exposure to TP setae induces local inflammatory reactions independent of earlier exposure to TP. The degree of reaction correlated well with the magnitude of symptoms during natural exposure. The initial reaction mimics a ‘foreign body reaction’ that varies depending on individual predisposition.
Salivary lactoferrin in a selected group of subjects with exceptional extrinsic dental staining
Authors: Nordbo H. , Kolsto A. B. , Eriksen H. M. 
Published: August 2010

Nordbø H, Kolstø AB, Eriksen HM. Salivary lactoferrin in a selected group of subjects with exceptional extrinsic dental staining. J Oral Pathol 1987: 16: 392–394.Some people exhibit an exceptional tendency to develop brownish extrinsic staining on their teeth and previous studies indicate that iron may be involved in certain types. The object of this investigation was to find out whether the level of salivary lactoferrin, which is an ironbinding glycoprotein, is elevated in persons exhibiting extreme staining tendency. Subjects who developed dark brownish discoloration on the facial surfaces of their anterior teeth during a 3‐week period following professional cleaning of the teeth were selected for study. Salivary lactoferrin was measured by the enzyme‐linked immunosorbent assay (ELISA). The results showed that this group of persons exhibited a markedly higher concentration of salivary lactoferrin compared with non‐stainers. It was also demonstrated in an in vitro study that combinations of lactoferrin, iron, and tannic acid produced stain on slabs of enamel and dentin.
Treatment of aphthous patients by enhancement of the salivary peroxidase system
Authors: Hoogendoorn H. , Piessens J. P. 
Published: August 2010

Hoogendoorn H, Piessens JP. Treatment of aphthous patients by enhancement of the salivary peroxidase system. J Oral Pathol 1987: 16: 425–427.The inhibition of glucose‐stimulated acid production by indigenous bacteria in human saliva is not achieved by the addition of up to 250 (μM hydrogen peroxide in vitro. However, in the presence of 2 × 10‐4% of hydroxyquinoline and the same amount of Zn, acid production is immediately terminated by addition of peroxide to only 25 μM. No inhibition is observed when any one of these components is omitted. On the basis of these observations, a mouthrinse containing the same concentrations of hydroxyquinoline and Zn was prepared. Hydrogen peroxide was provided by including glucose oxidase and amyloglucosidase. This mouthrinse was used in a pilot clinical study of 64 patients subject to severe aphthous attacks which were not previously relieved by the use of a peroxidogenic toothpaste. After a two‐month period, during which these patients rinsed twice daily with 5 ml of the mouthrinse, 45 patients reported relief of their symptoms. Of the remaining 19 patients, 17 reported no effect of using the mouthrinse, while 2 reported an exacerbation of their symptoms. The results of this study suggest that the mothrinse may be an effective method for treating patients who suffer from severe aphthous attacks.
p53 expression in oral precancer as a marker for malignant potential
Authors: Murti P. R. , Warnakulasuriya K. A. A. S. , Johnson N. W. , Bhonsle R. B. , Gupta P. C. , Daftary D. K. , Mehta F. S. 
Published: August 2010

ABSTRACTThe potential of p53 protein expression as a marker for determining which oral precancerous lesions may transform to malignancy with time was assessed. We compared the p53 expression in archival formalin‐fixed, paraffin‐embedded tissues from 22 baseline biopsies of precancerous lesions that transformed to cancer in 4‐25 years against that in 68 similar lesions that did not transform over the same time period. Twenty‐nine percent of precancers that transformed were p53‐positive at baseline, compared to 31% of the biopsies that did not transform to malignancy. When examined by immunohistochemical methods p53 expression failed to detect potential malignant status of oral precancer. Non‐specificity of the assay may account for this result but overexpression of p53 due to DNA damage by tobacco/betel‐quid in non‐progressive lesions needs further study. Nine precancerous lesions became p53‐immunoreactive from precancer to cancer. This may suggest p53 overexpression peaks close to the time of transition from precancer to cancer rather than early in the natural history of oral precancer.
Detection of Helicobacter pylori in various oral lesions by nested polymerase chain reaction (PCR)
Authors: Mravak‐Stipetić Marinka , Gall‐Tros̆elj Koraljka , Lukac̆ Josip , Kusić Zvonko , Pavelić Kres̆imir , Pavelić Jasminka 
Published: August 2010

Mravak‐Stipetic M, Gall‐Troselj K, Lukac J, Kusic Z, Pavelic K, Pavelic J: Detection of Helicobacter pylori in various oral lesions by nested polymerase chain reaction (PCR). J Oral Pathol Med 1998; 27: 1–3. © Munksgaard, 1998.Nested PCR was used for the detection of Helicobacter pylori DNA in specimens collected from seven different topographic sites in the oral cavity. Out of 161 patients, only 21 (13.04%) were positive. There was no correlation between H. pylori status and patient diagnosis and age. No preferential site for bacterial colonization was found in the oral cavity, nor was an association established between a bacterial presence and ulcerated versus non‐ulcerated lesions. The results indicate that the oral mucosa does not appear to represent a preferred site of colonization for H. pylori. Furthermore, the evidence presented in this paper suggests that H. pylori is not pathogenic in the oral cavity, nor is it associated with common oral pathologic processes.
Serine protease activity is essential for thrombin‐induced protein synthesis in cultured human dental pulp cells: modulation roles of prostaglandin E2
Authors: Chang Mei‐Chi , Lan Wan‐Hong , Chan Chiu‐Po , Lin Chun‐Pin , Hsieh Chi‐Chuan , Jeng Jiiang‐Huei 
Published: August 2010

Chang MC, Lan WH, Chan CP, Lin CP, Hsieh CC, Jeng JH: Serine protease activity is essential for thrombin‐induced protein synthesis in cultured human dental pulp cells: modulation roles of prostaglandin E2. J Oral Pathol Med 1998; 27: 23–9. © Munksgaard, 1998.Irritations and injuries to the dental pulp usually lead to different degrees of pulpal inflammation. To investigate the roles of thrombin and prostaglandins in the healing and inflammatory processes of dental pulp as well as their effects on pulpal protein synthesis, human dental pulp cell cultures were established and their protein production was measured with or without the presence of exogenous thrombin and prostaglandins. At concentrations of 1–25 U/ml, a‐thrombin increased the protein synthesis to 1.4–2.3 fold over the vehicle control. On the contrary, 0.1 (μg/ml of prostaglandin E] (PGE1 suppressed protein synthesis by 60%. Prostaglandin E2 (PGE2) also inhibited protein synthesis with an IC50 of 0.4 ug/ml. The stimulatory effects of thrombin (10 U/ml) can be inhibited by antithrombin III (2 U/ml) (a natural thrombin inhibitor) with heparin (2 U/ml), PPACK (D‐Phe‐Pro‐ArgCH2Cl) (20–50 ug/ml) (a serine protease inhibitor), and PGE2 (0.5–1.0 μg/ml). Moreover, TRAP (20–40 μg/ml), a thrombin receptor agonist peptide, also exerted a stimulatory effect (1.21–1.37 fold). In conclusion, thrombin‐induced protein synthesis by pulp cells is dependent on proteolytic activity, but not on binding to receptors. Both PGE1 and PGE2 exert suppressive effects on protein synthesis, indicating that interactions between thrombin and prostaglandins are important in regulating the inflammation, repair and regeneration of pulp tissue following injury.
Safety of salivary gland‐administered replication‐deficient recombinant adenovirus in rats
Authors: Delporte Christine , Miller Georgina , Kagami Hideaki , Lillibridge C. David , O'Connell Brian C. , Atkinson Jane C. , Baum Bruce J. 
Published: August 2010

Delporte C, Miller G, Kagami H, Lillibridge CD, O'Connell BC, Atkinson JC, Baum BJ: Safety of salivary gland‐administered replication‐deficient recombinant adenovirus in rats. J Oral Pathol Med 1998; 27: 34–8. © Munksgaard, 1998.We have examined the safety of a replication‐deficient recombinant adenovirus administered at a single, high dose intraductally to rat submandibular glands or systemically via the femoral vein. The virus used directed the synthesis of human aquaporin‐1, a water channel protein, and is termed AdhAQPl. Comparisons were made 1 and 9 days post‐infection with animals administered either a similar virus encoding no transgene or the viral suspension buffer. Animals were specifically not given anti‐inflammatory drugs to impede the well‐known immunopathologic response to recombinant adenoviral administration. Serum chemistries and hematological parameters were monitored. Rats were subjected to complete gross necropsy and selected tissues were evaluated by histopathology. Most clinical chemistry and hematology values were within normal ranges; however, evidence of inflammation (e.g., elevated lactic dehydrogenase, total leukocyte count) was seen. Gross pathology was normal, as was histopathology, excepting rare focal areas of necrosis. The results show that intrasalivary gland or intravenous AdhAQPl administration leads to low levels of toxicity in rats.
So‐called calcifying odontogenic cyst: review and discussion on the terminology and classification
Authors: Toida Makoto 
Published: August 2010

Toida M: So‐called calcifying odontogenic cyst: review and discussion on the terminology and classification. J Oral Pathol Med 1998; 27: 49–52. © Munks‐gaard, 1998.The so‐called calcifying odontogenic cyst (COC) shows extensive diversity in its clinico‐histopathological appearances and biological behaviour. Because of this diversity, there has been confusion and disagreement on the terminology and classification of this lesion. The attempts at classification of COC may be divided into two concepts. The first concept is the “monistic” one that all COCs are neoplastic in nature, even though the majority are cystic in architecture and appear to be non‐neoplastic. The second is the “dualistic” concept that COC contains two entities: a cyst and a neoplasm. Although the World Health Organization (WHO) classified COC as an odontogenic tumour in 1992 based on the former concept, current thinking favors strongly the latter one. In this article, several previous classifications of COC in the literature are discussed and a new simple classification scheme based on the “dualistic” concept is proposed.
Cytotoxic and non‐genotoxic effects of arecoline on human buccal fibroblasts in vitro
Authors: Chang Yu‐Chao , Tai Kuo‐Wei , Cheng Min‐Hsiung , Chou Lin Shin‐Shen , Chou Ming‐Yung 
Published: August 2010

Chang YC, Tai KW, Cheng MH, Chou LSS, Chou MY: Cytotoxic and non‐genotoxic effects of arecoline on human buccal fibroblasts in vitro. J Oral Pathol Med 1998; 27: 68–71. © Munksgaard, 1998.Betel quid chewing has been linked to oral submucous fibrosis and oral cancer. Cytotoxicity and genotoxicity assays were used to investigate the pathobiologi‐cal effects of arecoline on cultured human buccal fibroblasts. Arecoline increased double‐stranded polynucleic acid at the concentration of 0.1 to 10 μg/ml in a concentration‐dependent manner. At a concentration higher than 50 μg/ml, arecoline was cytotoxic to cultured fibroblasts and the cytotoxicity was dose‐dependent. No genotoxicity for arecoline was found even at a concentration of 400 μg/ml. On the other hand, 600 μg/ml glutathione (GSH) and 200 μg/ml glycyrrhizin could prevent the arecoline‐induced cytotoxicity. These results indicate that arecoline is a cytotoxic agent and no genotoxicity was found to human buccal fibroblasts. Furthermore, increasing consumption of GSH‐ and glycyrrhizin‐rich foods may reduce the oral diseases associated with betel quid chewing.
p53 aberrations in oral submucous fibrosis and oral squamous cell carcinoma detected by immunocytochemistry and PCR‐SSCP
Authors: Trivedy C. , Warnakulasuriya K. A. A. S. , Tavassoli M. , Steingrimsdottir H. , Penhallow J. , Maher R. , Johnson N. W. 
Published: August 2010

Trivedy C, Warnakulasuriya KAAS, Tavassoli M, Steingrimsdottir H, Penhallow J, Maher R, Johnson NW: p53 aberrations in oral submucous fibrosis and oral squamous cell carcinoma detected by immunocytochemistry and PCR‐SSCP. J Oral Pathol Med 1998; 27: 72–7. © Munksgaard, 1998.An archival series of oral biopsies from Karachi, Pakistan, consisting of 21 cases of oral submucous fibrosis (OSF) and 27 cases of squamous cell carcinoma (SCC), of which 6 had arisen from OSF, were used to examine the aberrations in the structure and expression of the p53 tumour suppressor gene. The PCR‐SSCP method was used for mutation analysis of exons 2–9, and (over)expression of p53 protein was detected by immunocytochemistry using monoclonal antibody DO 7. Positive immunostaining was observed in 15/20 (75%) of OSF specimens, 3/6 (50%) of SCC arising from OSF and 14/21 (67%) of SCC not arising from OSF. Mobility shifts in SSCP indicative of a mutation in p53 or loss of heterozygosity (deletion of a band) were seen in 13/21 cases of OSF and 15/27 cases of SCC. There was concordance between immunocytochemistry and SSCP results in a majority (33/48) of samples. Though the number of analysed SCC cases arising from OSF was limited, the results suggest that p53 mutation/protein stabilisation may play a part in the pathogenesis of OSF and its progression to SCC.
Immunohistochemical expression of p53, MDM2, Ki‐67 and PCNA in central giant cell granuloma and giant cell tumor
Authors: de Souza Paulo E. A. , O. Paim Júlia F. , Carvalhais Júnia Noronha , Gomez Ricardo S. 
Published: August 2010

ABSTRACTCentral gaint cell granuloma (CGCG) is a reactive bone lesion that occurs mainly in the jaws. The gaint cell tumour (GCT) is a benign locally aggressive neoplasm located near the articular end of tubular bones. Both lesions are characterised histologically by multinucleated gaint cells in a background of ovoid to spindle‐shaped mesenchymal cells. There is a basic question whether both lesions are separate entities or variants of the same disease. The study of cell cycle‐associated proteins may give insights into clarifing such question. The expression of these protiens is also important to determine the cell cycle regulation in both tumours. The purpose of this study was to evaluate the immunohistochimical expression of p53, MDM2, Ki‐67 and PCNA in CGCG and GCT. The results demonstrated that, despite the lack of p53 immunoreactivity, all the samples showed wide expression of MDM2. The percentage of Ki‐67‐and PCNA‐positive cell in CGCG was statistically higher than that of GCT. Our findings shwo that CGCG has a higher proliferative activity compared with that of the GCT. Our results also suggest that p53 inactivation by MDM2 expression may be involved in the pathogenesis of gaint cell lesions of the jaws and long bones.
DNA alterations in human oral squamous cell carcinomas detected by restriction landmark genomic scanning
Authors: Yamamoto K. , Konishi N. , Inui T. , Nakamura M. , Hiasa Y. , Kirita T. , Sugimura M. 
Published: August 2010

ABSTRACTGenetic abnormalities in human oral squamous cell carcinomas (OSCC) were examined using restriction landmark genomic scanning (RLGS), a method of two‐dimensional gel analysis allowing detection of amplifications and other aberrations in genomic DNA. DNAs from 11 oral tumours as well as from contiguous normal squamous epithelium, were cleaved with the restriction enzyme Not I, [32P] end‐labeled and electrophoretically size‐fractionated. Following a second digestion employing Hinf I, the further fragmented DNA was again electrophoretically separated. Five fragments/spots were found amplified in at least 64% (7/11) (chromosome nos. 4, 9–12 or 22) of carcinomas, with one of these spots amplified in 100% (chromosome no. 4) of tumour samples. In addition, six other spots were frequently reduced in at least 55% (chromosome nos. 15 or 9–12) of tumour tissues. Further characterization of these common changes is needed to determine if they represent important alterations in OSCC.
The in vitro post‐antifungal effect of nystatin on Candida species of oral origin
Authors: Ellepola A. N. B. , Samaranayake L. P. 
Published: August 2010

ABSTRACTThe post‐antifungal effect (PAFE) is defined as the suppression of growth that persists following limited exposure of yeasts to antimycotics and subsequent removal of the drug. Although limited data are available on the PAFE of nystatin on oral isolates of C. albicans, there is no information on non‐albicans Candida species. As nystatin is the commonest antifungal agent prescribed in dentistry, the main aim of this investigation was to measure the PAFE of oral isolates of Candida belonging to six different species (five isolates each of C. albicans, C. tropicalis, C. krusei, C. parapsilosis, C. glabrata and C. guilliermondii) following limited exposure (1 h) to nystatin. The yeasts were examined for the presence of the PAFE after 1 h exposure to the minimum inhibitory concentration (MIC) of nystatin. The PAFE was determined as the difference in time (h) required for the growth of the drug‐free control and the drug‐exposed test cultures to increase to the 0.05 absorbance level following removal of the antifungal agent. The mean duration of nystatin‐elicited PAFE was lowest for C. albicans (6.85 h) and greatest for C. parapsilosis (15.17 h), while C. krusei (11.58 h), C. tropicalis (12.73 h), C. glabrata (8.51 h), and C. guilliermondii (8.68 h) elicited intermediate values. These findings clarify another intriguing possibility for the persistent, chronic recurrence of oral C. albicans infections despite apparently adequate antifungal drug regimens. The significant variations in nystatin‐induced PAFE amongst non‐albicans species may also have clinical implications, in terms of nystatin regimens used in the management of these fungal infections.
PCNA/AgNOR and Ki‐67/ AgNOR double staining in oral squamous cell carcinoma
Authors: Costa Antonio de Lisboa Lopes , de Araújo Ney Soares , Jr Décio dos Santos Pinto , de Araújo Vera Cavalcanti 
Published: August 2010

Costa ALL, Araujo NS, Pinto Jr DS, Araujo VC: PCNA/AgNOR and Ki‐67/ AgNOR double staining in oral squamous cell carcinoma. J Oral Pathol Med 1999; 28: 438–41. © Munksgaard, 1999.This study was performed on oral squamous cell carcinomas (OSCC) in order to investigate the relation between the number of interphase silver‐stained nucleolar organizer regions (AgNORs) and the immunolabeling of proliferation‐associated markers, using antibodies to Ki‐67 and proliferating cell nuclear antigen (PCNA). Fifteen consecutive cases of oral squamous cell carcinoma were used and a double staining technique was performed in order to quantify the number of NORs in PCNA‐positive and ‐negative cells as well as in Ki‐67‐positive and ‐negative cells. Our results showed a higher mean number of AgNORs in PCNA‐and Ki‐67‐positive cells than in PCNA‐ and Ki‐67‐negative cells. We concluded that there is an association between cell proliferation and AgNOR score in OSCC.
Biosynthesis of glycosaminoglycans and aggrecan by tumor cells in salivary pleomorphic adenoma: ultrastructural evidence
Authors: Zhao Ming , Takata Takashi , Kudo Yasusei , Sato Sunao , Ogawa Ikuko , Wakida Kazuyoshi , Uchida Takashi , Nikai Hiromasa 
Published: August 2010

Zhao M, Takata T, Kudo Y, Sato S, Ogawa I, Wakida K, Uchida T, Nikai H: Biosynthesis of glycosaminoglycans and aggrecan by tumor cells in salivary pleomorphic adenoma: ultrastructural evidence. J Oral Pathol Med 1999; 28: 442–50. © Munksgaard, 1999.The present study attempted to discover the sites of synthesis of various glycosaminoglycans (GAGs) and aggrecan in salivary pleomorphic adenoma (PA) with the use of a highly sensitive and specific post‐embedding immunogold‐silver staining technique at the ultrastructural level. Silver particles representing various GAGs and aggrecan were found to accumulate frequently in the intercellular spaces of non‐luminal cells in the epithelial clusters and were dispersed in the myxoid matrix of the mesenchyme‐like areas. Furthermore, the non‐luminal epithelial cells were demonstrated to contain immunopositive intracytoplasmic vesicles and vacuoles, some of which were of Golgi complex origin. In contrast, intracellular silver particles for hyaluronic acid were mostly attached to the inner surface of the cell membrane. These observations agree well with the current theories of the biosynthesis of GAGs and proteoglycans and provide direct evidence for the production of various GAGs and aggrecan by tumor epithelial cells of PA. Such findings support the ideas that in PA a loss of epithelium occurs by stromali‐zation following epithelial secretion of extracellular matrix substances and transformation of epithelium to mesenchyme represents the basic principle of the tissue heterogeneity in this tumor.
Crystalloid architecture of a sialolith in a minor salivary gland
Authors: Riesco J. M. , Juanes J. A. , Diaz‐Gonzalez M. P. , Blanco E. J. , Riesco‐Lopez J. M. , Vazquez R. 
Published: August 2010

Riesco JM, Juanes JA, Díaz‐González MP, Blanco EJ, Riesco‐Löpez JM, Vázquez R: Crystalloid architecture of a sialolith in a minor salivary gland. J Oral Pathol Med 1999; 28: 451–5. © Munksgaard, 1999.A sialolith from a minor salivary gland of the mucosa of the upper lip was studied morphologically and analytically. Under stereoscopic microscopic visualisation, no core or any concentric laminar structure was found in the sialolith and it had a transparent glassy appearance. Scanning electron microscopy revealed differences between the internal structure of the sialolith and its external structure. No microbes were observed but some mineralized inclusion bodies were seen. X‐ray diffraction indicated the absence of inorganic crystals in the sialolith, while energy dispersive x‐ray microanalysis revealed a high content of S followed by Ca, Si and Na and little and scarce P on the fracture surface, with S, Na, Ca and P being distributed throughout the external coating of the sialolith. The results suggest that the sialolith was young and consisted of a crystalloid body with an incipient coating undergoing calcification.