bluuebunny/crossref_metadata_2025_split huggingface dataset:
Successful salvage therapy with Daptomycin for osteomyelitis caused by methicillin-resistant Staphylococcus aureus in a renal transplant recipient with Fabry-Anderson disease
Authors: Polilli Ennio , Ursini Tamara , Mazzotta Elena , Sozio Federica , Savini Vincenzo , D'Antonio Domenico , Barbato Michelino , Consorte Augusta , Parruti Giustino 
Published: March 2012

Abstract
Daptomycin is licensed in adults for the management of Staphylococcus aureus methicillin-resistant infections, including bone and skin complicated infections. We describe for the first time its use in a renal transplant recipient for Fabry-Anderson Disease with right heel osteomyelitis. The patient was unresponsive to first-line Teicoplanin and second-line Tigecycline, whereas he was successfully treated with third-line Daptomycin monotherapy at 4 mg/Kg/qd for 4 weeks. Local debridement was performed in advance of each line of treatment.
Histological spectrum of pulmonary manifestations in kidney transplant recipients on sirolimus inclusive immunosuppressive regimens
Authors: Kirby Sean , Satoskar Anjali , Brodsky Sergey , Pope-Harman Amy , Nunley David , Hitchcock Charles , Pelletier Ronald , Ross Patrick , Nadasdy Tibor , Shilo Konstantin 
Published: March 2012

Abstract

Background
After the introduction of novel effective immunosuppressive therapies, kidney transplantation became the treatment of choice for end stage renal disease. While these new therapies lead to better graft survival, they can also cause a variety of complications. Only small series or case reports describe pulmonary pathology in renal allograft recipients on mTOR inhibitor inclusive therapies. The goal of this study was to provide a systematic review of thoracic biopsies in kidney transplant recipients for possible association between a type of immunosuppressive regimen and pulmonary complications.

Methods
A laboratory database search revealed 28 of 2140 renal allograft recipients (18 males and 10 females, 25 to 77 years old, mean age 53 years) who required a biopsy for respiratory symptoms. The histological features were correlated with clinical findings including immunosuppressive medications.

Results
The incidence of neoplasia on lung biopsy was 0.4% (9 cases), which included 3 squamous cell carcinomas, 2 adenocarcinomas, 1 diffuse large B-cell lymphoma, 1 lymphomatoid granulomatosis, and 2 post transplant B-cell lymphoproliferative disorders. Diffuse parenchymal lung disease was identified in 0.4% (9 cases), and included 5 cases of pulmonary hemorrhage, 3 cases of organizing pneumonia and 1 case of pulmonary alveolar proteinosis. Five (0.2%) cases showed histological features indicative of a localized infectious process. Patients on sirolimus had neoplasia less frequently than patients on other immunosuppressive combinations (12.5% vs. 58.3%, p = 0.03). Lung biopsies in 4 of 5 patients with clinically suspected sirolimus toxicity revealed pulmonary hemorrhage as the sole histological finding or in combination with other patterns.

Conclusions
Our study documents a spectrum of neoplastic and non-neoplastic lesions in renal allograft recipients on current immunosuppressive therapies. Sirolimus inclusive regimens are associated with increased risk of pulmonary toxicity but may be beneficial in cases of posttransplant neoplasia.

Virtual Slides
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/3320012126569395.
lemmingA encodes the Apc11 subunit of the APC/C in Drosophila melanogaster that forms a ternary complex with the E2-C type ubiquitin conjugating enzyme, Vihar and Morula/Apc2
Authors: Nagy Olga , Pál Margit , Udvardy Andor , Shirras Christine AM , Boros Imre , Shirras Alan D , Deák Péter 
Published: March 2012

Abstract

Background
Ubiquitin-dependent protein degradation is a critical step in key cell cycle events, such as metaphase-anaphase transition and mitotic exit. The anaphase promoting complex/cyclosome (APC/C) plays a pivotal role in these transitions by recognizing and marking regulatory proteins for proteasomal degradation. Its overall structure and function has been elucidated mostly in yeasts and mammalian cell lines. The APC/C is, however, a multisubunit assembly with at least 13 subunits and their function and interaction within the complex is still relatively uncharacterized, particularly in metazoan systems. Here, lemming (lmg) mutants were used to study the APC/C subunit, Apc11, and its interaction partners in Drosophila melanogaster.

Results
The lmg gene was initially identified through a pharate adult lethal P element insertion mutation expressing developmental abnormalities and widespread apoptosis in larval imaginal discs and pupal abdominal histoblasts. Larval neuroblasts were observed to arrest mitosis in a metaphase-like state with highly condensed, scattered chromosomes and frequent polyploidy. These neuroblasts contain high levels of both cyclin A and cyclin B. The lmg gene was cloned by virtue of the lmg

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P element insertion which is located in the 5' untranslated region. The lemming locus is transcribed to give a 2.0 kb mRNA that contains two ORFs, lmgA and lmgB. The lmgA ORF codes for a putative protein with more than 80% sequence homology to the APC11 subunit of the human APC/C. The 85 amino acid protein also contains a RING-finger motif characteristic of known APC11 subunits. The lmgA ORF alone was sufficient to rescue the lethal and mitotic phenotypes of the lmg

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null allele and to complement the temperature sensitive lethal phenotype of the APC11-myc9 budding yeast mutant. The LmgA protein interacts with Mr/Apc2, and they together form a binding site for Vihar, the E2-C type ubiquitin conjugating enzyme. Despite being conserved among Drosophila species, the LmgB protein is not required for viability or fertility.

Conclusions
Our work provides insight into the subunit structure of the Drosophila APC/C with implications for its function. Based on the presented data, we suggest that the Lmg/Apc11 subunit recruits the E2-C type ubiquitin conjugating enzyme, Vihar, to the APC/C together with Mr/Apc2 by forming a ternary complex.
Recent updates on the role of microRNAs in prostate cancer
Authors: Hassan Oudai , Ahmad Aamir , Sethi Seema , Sarkar Fazlul H 
Published: March 2012

AbstractMicroRNAs (miRNAs) are short non-coding RNAs that are involved in several important biological processes through regulation of genes post-transcriptionally. Carcinogenesis is one of the key biological processes where miRNAs play important role in the regulation of genes. The miRNAs elicit their effects by binding to the 3' untranslated region (3'UTR) of their target mRNAs, leading to the inhibition of translation or the degradation of the mRNA, depending on the degree of complementary base pairing. To-date more than 1,000 miRNAs are postulated to exist, although the field is moving rapidly. Currently, miRNAs are becoming the center of interest in a number of research areas, particularly in oncology, as documented by exponential growth in publications in the last decade. These studies have shown that miRNAs are deregulated in a wide variety of human cancers. Thus, it is reasonable to ask the question whether further understanding on the role of miRNAs could be useful for diagnosis, prognosis and predicting therapeutic response for prostate cancer (PCa). Therefore, in this review article, we will discuss the potential roles of different miRNAs in PCa in order to provide up-to-date information, which is expected to stimulate further research in the field for realizing the benefit of miRNA-targeted therapeutic approach for the treatment of metastatic castrate resistant prostate cancer (mCRPC) in the near future because there is no curative treatment for mCRPC at the moment.
A novel fault tolerant service selection framework for pervasive computing
Authors: Silas Salaja , Ezra Kirubakaran , Blessing Rajsingh Elijah 
Published: March 2012

Abstract
Background
Service selection in pervasive computing is significant as it requires identifying the best service provider based on users requirements. After identifying the best service provider, when a service is being executed, service disruptions happen due to various faults.

Methods
Though attempts are made to provide best services to the user, executing the service without service disruptions becomes an important challenge in the pervasive environment. In this paper, a novel Fault tolerant Service Selection Framework (FTSSF) has been proposed.

Results
Adequate theoretical analysis was carried out and experimental results were obtained for the proposed framework and have been compared with the existing techniques.

Conclusion
The results prove that the proposed framework is efficient and fault tolerant.
TLR2 ligation induces the production of IL-23/IL-17 via IL-6, STAT3 and NF-kB pathway in patients with primary Sjogren's syndrome
Authors: Kwok Seung-Ki , Cho Mi-La , Her Yang-Mi , Oh Hye-Joa , Park Mi-Kyung , Lee Seon-Yeong , Woo Yun Ju , Ju Ji Hyeon , Park Kyung-Su , Kim Ho-Youn , Park Sung-Hwan 
Published: March 2012

Abstract
Introduction
The study was undertaken to investigate the interrelation of toll-like receptor (TLR) and interleukin (IL)-17 in the salivary glands of patients with primary Sjogren's syndrome (pSS) and to determine the role of TLR and IL-17 in the pathophysiology of pSS.

Methods
The expressions of various TLRs, IL-17 and the cytokines involved in Th17 cell differentiation including IL-6, IL-23, tumor necrosis factor-alpha (TNF-α) and IL-1β were examined by immunohistochemistry in salivary glands of pSS patients. The IL-17 producing CD4+ T cells (Th17 cells) were examined by flow cytometry and confocal staining in peripheral mononuclear blood cells (PMBCs) and salivary glands of pSS patients. After PBMCs were treated with TLR specific ligands, the induction of IL-17 and IL-23 was determined using real-time PCR and ELISA. The signaling pathway that mediates the TLR2 stimulated production of IL-17 and IL-23 was investigated by using treatment with specific signaling inhibitors.

Results
We showed that TLR2, TLR4, TLR6, IL-17 and the cytokines associated with Th17 cells were highly expressed in salivary glands of pSS patients but not in controls. The expressions of TLR2, TLR4 and TLR6 were observed in the infiltrating mononuclear cells and ductal epithelial cells, whereas IL-17 was mainly observed in infiltrating CD4+ T cells. The number of IL-17 producing CD4+ T cells was significantly higher in pSS patients both in PBMCs and minor salivary glands. The stimulation of TLR2, TLR4 and TLR6 additively induced the production of IL-17 and IL-23 from the PBMCs of pSS patients especially in the presence of TLR2 stimulation. IL-6, signal transducer and activator of transcription 3 (STAT3) and nuclear factor-kappaB (NF-kB) pathways were implicated in the TLR2 stimulated IL-17 and IL-23.

Conclusions
Our data demonstrate that TLR2 ligation induces the production of IL-23/IL-17 via IL-6, STAT3 and NF-kB pathway in pSS. Therefore, therapeutic strategies that target TLR/IL-17 pathway might be strong candidates for treatment modalities of pSS.
Granzyme B- and Fas ligand-mediated cytotoxic function induced by mitogenic CD28 stimulation of human memory CD4+ T cells
Authors: Medina Miguel A , Couturier Jacob , Feske Marsha L , Mahne Ashley E , Turner Mary , Yu Xiaoyong , Kozinetz Claudia A , Orozco Aaron F , Hutchison Alexander T , Savidge Tor C , Rodgers John R , Lewis Dorothy E 
Published: March 2012

ABSTRACT
Some human memory CD4+ T cells have cytotoxic functions best understood in the context of viral infections; however, their possible role in pathologic processes is understudied. The novel discovery that mitogenic CD28 antibodies induced proliferation and expansion of Tregs offered therapeutic promise for autoimmune disorders. However, the failed TGN1412 trial forced reassessment of this concept. As memory CD4+ T cells are known to produce toxic molecules, including granzyme B (GrzB) and FasL, we wondered whether mitogenic CD28 was able to induce these cytotoxic molecules. A commercially available mitogenic human CD28 mAb (clone ANC28.1) was used to determine whether mitogenic CD28 induces cytotoxic function from human memory CD4+ T cells. We found that stimulation of memory CD4+ T cells by ANC28.1, as well as by conventional costimulation (CD3/CD28 mAb), robustly induced enzymatically active GrzB, along with increased surface expression of FasL. These functional phenotypes were induced in association with increased expression of T cell activation markers CD69 and CD25, and elimination of target cells by ANC28.1-activated memory CD4+ T cells involved both GrzB and FasL. Additionally, ANC28.1-activated memory CD4+ T cells caused disruption of epithelial cell monolayer integrity, which was partially mediated by GrzB. These findings reveal functions of memory CD4+ T cells previously unknown to be induced by mitogenic CD28, and suggest that these pathogenic mechanisms may have been responsible for some of the widespread tissue destruction that occurred in the TGN1412 trial recipients.
Early IL-10 production is essential for syngeneic graft acceptance
Authors: Takiishi Tatiana , Tadokoro Carlos Eduardo , Rizzo Luiz Vicente , de Moraes Luciana Vieira 
Published: March 2012

Abstract
IL-10 production by donor epithelial cells immediately upon transplantation is essential for syngeneic skin graft acceptance.
We performed a comparative study and evaluated cellular infiltrates and anti-inflammatory cytokine production at different time-points after syngeneic or allogeneic skin transplantation. We observed an early IL-10 production in syngeneic grafts compared with allografts. This observation prompted us to investigate the role of IL-10 in isograft acceptance. For this, we used IL-10 KO and WT mice to perform syngeneic transplantation, where IL-10 was absent in the graft or in the recipient. The majority of syngeneic grafts derived from IL-10 KO donors did not engraft or was only partially accepted, whereas IL-10 KO mice transplanted with skin from WT donors accepted the graft. We evaluated IL-10 producers in the transplanted skin and observed that epithelial cells were the major source. Taken together, our data show that production of IL-10 by donor cells, but not by the recipient, is determinant for graft acceptance and strongly suggest that production of this cytokine by keratinocytes immediately upon transplantation is necessary for isograft survival.
The value of diffusion tensor imaging in the differential diagnosis of subcortical ischemic vascular dementia and Alzheimer's disease in patients with only mild white matter alterations on T2-weighted images
Authors: Fu Jian-Liang , Zhang Ting , Chang Cheng , Zhang Yu-Zhen , Li Wen-Bin 
Published: March 2012

Background Diffusion tensor imaging (DTI) is a form of functional magnetic resonance imaging (MRI) that allows examination of the microstructural integrity of white matter in the brain. Dementia is a neurodegenerative disease, and DTI can provide indirect insights of the microstructural characteristics of brains in individuals with different forms of dementia. Purpose To evaluate the value of DTI in the diagnosis and differential diagnosis of patients with subcortical ischemic vascular dementia (SIVD) and Alzheimer's disease (AD). Material and Methods The study included 40 patients (20 AD patients and 20 SIVD patients) and 20 normal controls (NC). After routine MRI and DTI, fractional anisotropy (FA) and apparent diffusion coefficient (ADC) values were measured and compared in regions of interest (ROI). Results Compared to NC and AD patients, SIVD patients had lower FA values and higher ADC values in the inferior-fronto-occipital fascicles (IFOF), genu of the corpus callosum (GCC), splenium of the corpus callosum (SCC), and superior longitudinal fasciculus (SLF). Compared to controls and SIVD patients, AD patients had lower FA values in the anterior frontal lobe, temporal lobe, hippocampus, IFOF, GCC, and CF; and higher ADC values in the temporal lobe and hippocampus. Conclusion DTI can be used to estimate the white matter impairment in dementia patients. There were significant regional reductions of FA values and heightened ADC values in multiple regions in SIVD patients compared to AD patients. When compared with conventional MRI, DTI may provide a more objective method for the differential diagnosis of SIVD and AD disease patients who have only mild white matter alterations on T2-weighted imaging.
Differential diagnosis of vertebral compression fracture using in-phase/opposed-phase and short TI inversion recovery imaging
Authors: Ogura Akio , Hayakawa Katsumi , Maeda Fumie , Saeki Fumito , Syukutani Ai , Shibutani Sachiko , Kuroda Emi 
Published: March 2012

Background Magnetic resonance imaging (MRI) is highly useful for detecting diseases of the bone marrow. The sensitivity for detecting compression fracture is very high, but specificity is low for differential diagnosis between malignant and benign cases. Purpose To evaluate the usefulness of in-phase/opposed-phase and short TI inversion recovery (STIR) magnetic resonance imaging (MRI) of bone marrow for differentiation between benign and malignant vertebral compression fractures. Material and Methods A retrospective review of 66 patients with 70 vertebral compression fractures was performed. The signal intensity ratio (SIR) defined as SIR (opposed/in) was calculated from in-phase/opposed-phase MR images, and the signal intensity ratio as SIR (STIR) was calculated from STIR MR images. The relationships between values of SIR (opposed/in) and SIR (STIR) and the differential diagnosis of malignant vs. benign fractures were considered. Results When SIR (opposed/in) was less than 1.0, bone marrow was benign. The bone marrow was malignant when both SIR (opposed/in) was greater than 1.0 and SIR (STIR) was less than 2.0. Conclusion In cases of acute compression fracture, malignant bone marrow showed SIR(STIR) values less than 2.0 and SIR (in/opposed) greater than 1.0. In contrast, benign bone marrow showed SIR (STIR) values greater than 2.5. For chronic compression fracture, malignant bone marrow showed SIR (in/opposed) greater than 1.0. Bone marrow was benign in all cases with SIR (in/opposed) less than 1.0.
Making History in Africa: David Henige and the Quest for Method in African History
Authors: Doortmont Michel R. 
Published: March 2012

My own history with David Henige goes back to 1985, when I had just finished a master's degree in African studies at the Centre of West African Studies in Birmingham, England, and was looking for a place and a supervisor for a planned doctoral dissertation involving a historiographical study of Nigeria. One of my supervisors, Tom McCaskie, suggested getting in touch with Henige, to see if he could assist me. The reply was elaborate and positive, which I appreciated much. Circumstances for graduate students at the time being quite different from the present, and funding systems for study abroad still in their infancy, the plan came to nothing. The connection with Henige and his work was there to stay, however.This article is an effort to give a reflection on David Henige's career and his impact on the discipline of history in Africa, through his work as editor of History in Africa. The scope of the reflection is limited, as we concentrate on David's own contributions, rather than setting him and his work in a comparative framework. When David Henige started History in Africa in 1974, it was yet another scholarly journal on Africa, in an ever-growing series, counting already more than two hundred titles, as Henige pointed out himself. And indeed, in such circumstances, a new journal needs ‘to justify itself to the audience it addresses.’
From the Madrasah to the Museum: The Social Life of the “Kietaabs” of Cape Town
Authors: Jappie Saarah 
Published: March 2012

Ebrahiem Manuel sits opposite me, about to embark upon his story. His living room is filled with material manifestations of his research: boxes overflowing with books and papers cover his entire sofa, newspapers and articles line the floor, and collages of images and texts hang on the walls and sit in the cabinets. It is clear that he is consumed by his passion for heritage, and his personal journey of discovery. He speaks in an animated, almost theatrical tone, raising and lowering his voice, stressing certain syllables, alive as he tells his story of “the ancient kietaabs.”The journey began in 1997, when Ebrahiem returned to South Africa after years at sea, working as a cook on shipping vessels. Upon his return, he began a quest to learn about his personal heritage, inspired by a dream he had had about his grandfather. This search led him to an oldkietaab, given to him by an elderly aunt. This was not the first time he had come across the old book; he remembered seeing it as a child, amongst other kietaabs, stored out of reach of the children, on top of his grandfather's wardrobe. It was inside this book that a possible key to his ancestors was to be found.This significant find was a range of hand-written inscriptions inside the book, in Arabic, English, and an unknown script. The Arabic script and its corresponding English transliteration read “Imaam Abdul Karriem, son of Imaam Abdul Jaliel, son of Imaam Ismail of Sumbawa.” Here was his family tree, starting from his great-grandfather and leading to two generations before him and, it seemed, their place of origin, the island of Sumbawa in eastern Indonesia. Ebrahiem then decided to go to Indonesia to solve what had become the mystery of “the ancient kietaab.”
Local Knowledge: An Akuapem Twi History of Asante
Authors: McCaskie Tom C. 
Published: March 2012

In 2003 Asantehene Agyeman Prempeh I's eighty-nine page manuscript ‘The History of Ashanti Kings and the whole country itself’ of 1907 was published in an annotated scholarly edition alongside a selection of allied texts. The same publisher is to produce a related volume containing the four hundred and fifty pages of Asantehene Osei Agyeman Prempeh II's ‘History of Ashanti’ written in the 1940s (and edited by myself). Both of these texts are written in English. However, the huge range of sources on the Asante past recorded in Akan Twi have yet to receive equal attention and treatment. This short paper introduces and contextualises one source of this kind that was researched in Asante between 1902-1910 and finished in written form in Akan Twi in 1915.The Akuapem (Akwapim) kingdom is located less than thirty miles northeast of Ghana's capital at Accra. It has always been and remains a small polity. It comprises only seventeen historic towns scattered among hills on two parallel ridges about fifteen hundred feet above sea level. There are more towns today, many created by the cocoa economy of the early twentieth century, but Akuapem remains a compact entity. It is a Twi-speaking Akan kingdom, but an unusual one in that it is ethnically diverse.Patrilineal Guan-speaking farmers settled on the Akuapem ridges in the early decades of the seventeenth century. They were oppressed by the matrilineal Twi-speaking Akan of the nearby Akwamu kingdom. To end this situation the Guan recruited other Akan Twi speakers as allies. These were military adventurers from the Akyem Abuakwa polity to the west. The Akyem incomers succeeded against the Akwamu but stayed on to establish their own conquest dynasty in 1733.