bluuebunny/crossref_metadata_2025_split huggingface dataset:
BST‐2 binding with cellular MT1‐MMP blocks cell growth and migration via decreasing MMP2 activity
Authors: Gu Gongping , Zhao Dejian , Yin Ziming , Liu Ping 
Published: November 2011

AbstractMT1‐MMP (membrane type 1‐matrix metalloproteinase) plays important roles in cell growth and tumor invasion via mediating cleavage of MMP2/gelatinase A and a variety of substrates including type I collagen. BST‐2 (bone marrow stromal cell antigen 2) is a membrane tetherin whose expression dramatically reduces the release of a broad range of enveloped viruses including HIV from infected cells. In this study, we provided evidence that both transient and IFN‐α induced BST‐2 could decrease the activity of MMP2 via binding to cellular MT1‐MMP on its C‐terminus and inhibiting its proteolytic activity; and finally block cell growth and migration. Zymography gel and Western blot experiments demonstrated that BST‐2 decreased MMP2 activity, but no effect on the expression of MMP2 and MT1‐MMP genes. Confocal and immunoprecipitation data showed that BST‐2 co‐localized and interacted with MT1‐MMP. This interaction inhibited the proteolytic enzyme activity of MT1‐MMP, and blocked the activation of proMMP2. Experimental results of C‐terminus deletion mutant of MT1‐MMP showed that activity of MMP2 was no change and also no interaction existed between the mutant and BST‐2 after co‐transfection with the mutant and BST‐2. It meant that C‐terminus of MT1‐MMP played a key role in the interaction with BST‐2. In addition, cell growth in 3D type I collagen gel lattice and cell migration were all inhibited by BST‐2. Taken together, BST‐2, as a membrane protein and a tetherin of enveloped viruses, was a novel inhibitor of MT1‐MMP and could be considerable as an inhibitor of cancer cell growth and migration on clinic. J. Cell. Biochem. 113: 1013–1021, 2012. © 2011 Wiley Periodicals, Inc.
Insomnia as predictor versus outcome of PTSD and depression among Iraq combat veterans
Authors: Wright Kathleen M. , Britt Thomas W. , Bliese Paul D. , Adler Amy B. , Picchioni Dante , Moore DeWayne 
Published: November 2011

AbstractObjectives: The study conducted a longitudinal assessment of insomnia as an antecedent versus consequence of posttraumatic stress disorder (PTSD) and depression symptoms among combat veterans. Design: Two postdeployment time points were used in combination with structural equation modeling to examine the relative strength of two possible directions of prediction: insomnia as a predictor of psychological symptoms, and psychological symptoms as a predictor of insomnia. Participants were active duty soldiers (N = 659) in a brigade combat team who were assessed 4 months after their return from a 12‐month deployment to Iraq, and then again eight months later. Results: Although both insomnia and psychological symptoms were associated at both time periods and across time periods, insomnia at 4 months postdeployment was a significant predictor of change in depression and PTSD symptoms at 12 months postdeployment, whereas depression and PTSD symptoms at 4 months postdeployment were not significant predictors of change in insomnia at 12 months postdeployment. Conclusions: Results support the role of insomnia in the development of additional psychological problems and highlight the clinical implications for combat veterans, to include the importance of longitudinal assessment and monitoring of sleep disturbances, and the need for early intervention. © 2011 Wiley Periodicals, Inc. J Clin Psychol 67:1–19, 2011.
Effects of exposition to polluted environments on blood cells of the fish Prochilodus lineatus
Authors: Pereira Bruno Fiorelini , Da Silva Alves Rebeca Mamede , Pitol Dimitrius Leonardo , Senhorini José Augusto , De Cássia Gimenes De Alcântara Rocha Rita , Caetano Flavio Henrique 
Published: November 2011

AbstractIn this study, we investigated the effects of contaminated water on the blood parameters of the fish Prochilodus lineatus exposed during 7 and 20 days. Blood was collected with heparinized syringes, and blood smears were prepared and stained with Leishman stain. Slides were examined and photographed using a Leica light microscope. Total white blood cell counts and differential counts of thrombocytes and leukocytes were obtained for statistical analysis. The group exposed to water samples from Lago Azul exhibited an increase in the number of leukocytes and in the total number of white blood cells, suggesting that the chemical contaminants in this environment were acting similarly to antigens in this fish species, causing the proliferation of defense cells. In the group exposed to detergent during 20 days, the number of thrombocytes decreased. These results suggest that the variations in the number of leukocytes were indicators of environmental pollution and that biodegradable detergents may, after a certain time of exposure, affect vital functions in fish, such as coagulation and prevention of infections, which directly involves thrombocytes. Microsc. Res. Tech., 2012. © 2011 Wiley Periodicals, Inc.
Facilitating the pickup of individual DNA molecules by AFM nanomanipulation with tips mechanically worn on bare mica
Authors: Duan Na , Long Fei , Wang Xinyan , Li Bin , Hu Jun , Zhang Yi 
Published: November 2011

AbstractThe tip is one of the critical factors to improve the efficiency in picking up individual DNA molecules from solid substrates based on atomic force microscope (AFM) nanomanipulation. We found that wearing AFM tips on certain solid substrates in advance to nanomanipulation operation would largely improve the pickup efficiency, which was ascribed to the increasing affinity of the tip to the DNA molecules along with the increase of the tip radius after wearing. It was demonstrated that bare mica was superior to APTES‐modified mica to keep the tip clean while wearing, which was crucial for DNA pickup during AFM nanomanipulation. Microsc. Res. Tech., 2012. © 2011 Wiley Periodicals, Inc.
Self‐etching adhesive on intact enamel, with and without pre‐etching
Authors: Devarasa G.M. , Subba Reddy V.V. , Chaitra N.L. , Swarna Y.M. 
Published: November 2011

AbstractOBJECTIVE:Bond strengths of composite resin to enamel using self‐etch adhesive (SEA) Clearfil SE bond system on intact enamel and enamel pre‐etched with phosphoric acid were compared. The objective was to determine if the pre‐etching would increase the bond strengths of the SEA systems to intact enamel and to evaluate the effect of pre‐etching on bond formation of self‐etch adhesives on intact enamel.MATERIALS AND METHOD:Labial surfaces of 40 caries free permanent upper central and lateral incisors were cleaned, sectioned of their roots. All specimens were mounted on acrylic block and divided randomly into four groups. In two groups the application of self‐etch adhesive, Clearfil SE bond was carried as per manufacturer's instructions, composite cylinders were built, whereas in the other two groups, 37% phosphoric acid etching was done before the application of self‐etching adhesives. Then the resin tags were analyzed using scanning electron microscope and shear bond strength was measured using Instron universal testing machine.RESULTS:When phosphoric acid was used, there was significant increase in the depth of penetration of resin tags and in the Shear Bond Strength of composite to enamel.CONCLUSION:The results indicate that out of both treatment groups, pre‐etching the intact enamel with 37% phosphoric acid resulted in formation of longer resin tags and higher depth of penetration of resin tags of the Clearfil SE bond, and attaining higher bond strength of the Clearfil SE bond to intact enamel. Microsc. Res. Tech., 2012. © 2011 Wiley Periodicals, Inc.
The influence of ENSO on winter rainfall in South Africa
Authors: Philippon N. , Rouault M. , Richard Y. , Favre A. 
Published: November 2011

AbstractWhereas the impact of ENSO on the African summer rainfall regions is largely documented and still regularly investigated, little is known about its impact on the winter rainfall regions located at the southwestern and northwestern tips of Africa. Yet, these regions are densely inhabited and are net exporters of high‐quality agricultural products. Here we analyze the relationship between El Niño Southern Oscillation (ENSO) and South Africa austral winter rainfall using a 682 raingauges daily rainfall database documenting the period 1950–1999. The May, June and July (MJJ) seasonal rainfall amount shows a positive correlation with the Niño3.4 index that becomes significant since the so‐called 1976/1977 climate regime shift. Wet spells properties (length, frequency and intensity) at the raingauge scale indicate that high (low) MJJ seasonal rainfall amounts recorded during El Niño (La Niña) events are the result of longer (shorter) wet spells in the Cape Town area and more (less) frequent wet spells north of 33°S. Wet spells with daily rainfall amounts ranging between 10 and 50 mm are also more (less) frequent. Atmospheric dynamics fields during wet spells feature lower (higher) pressure and northwesterly (southerly) wind anomalies in the troposphere over the region. This suggests that rain‐bearing systems are deeper (thinner) and larger (smaller) in extent, and located farther north (south) during El Niño (La Niña) events. Copyright © 2011 Royal Meteorological Society
Influence of uncommon histology on breast conservation therapy for breast cancer‐biology dictates technique?
Authors: Wasif Nabil , McCullough Ann E. , Gray Richard J. , Pockaj Barbara A. 
Published: November 2011

AbstractIntroductionAlthough trends and variations in the use of breast conservation therapy (BCT) for ductal carcinoma have been studied, little is known about uncommon breast cancer histologies.MethodsThe Surveillance, Epidemiology and End Results (SEER) database was used to identify 338,682 patients with T1 or T2 (≤5 cm) ductal, lobular, tubular, mucinous, medullary, or papillary carcinoma of the breast from 1998 to 2008. Multivariate logistic regression analysis was used to identify predictors of BCT.ResultsThe majority of patients underwent BCT (60%). The rate of BCT remained relatively constant from 1998 to 2008 overall but varied from 50% for lobular to 79% for tubular. The highest rate of mastectomy was seen in lobular (49%). Nodal positivity following surgical staging was lowest for tubular (6%) and mucinous (8%). Adjuvant radiation was given to 72% overall and was lowest for papillary (58%). Predictors of BCT included tubular (OR 1.8, 95% CI 1.7–1.9) and medullary (OR 2.0, 95% CI 1.8–2.2) subtypes (vs. ductal).ConclusionsPatients with uncommon breast cancer histologies show wide variation in the application of BCT depending on the primary tumor. This suggests that an individualized approach in the use of BCT depending on histology should be used. J. Surg. Oncol. 2012; 105:586–590. © 2011 Wiley Periodicals, Inc.
The influence of postoperative glycemic control on recurrence after curative resection in diabetics with hepatitis C virus‐related hepatocellular carcinoma
Authors: Kaneda Kazuhisa , Uenishi Takahiro , Takemura Shigekazu , Shinkawa Hiroji , Urata Yorihisa , Sakae Masayuki , Yamamoto Takatsugu , Kubo Shoji 
Published: November 2011

AbstractBackground and ObjectivesTo elucidate the influence of diabetes on tumor recurrence after curative resection for hepatitis C virus (HCV)‐related hepatocellular carcinoma (HCC).MethodsA total of 100 patients who underwent curative resection for solitary HCV‐related HCC were analyzed. Data from 26 patients with diabetes were compared to those of 74 patients without diabetes. The two groups were matched in terms of presence of cirrhosis, Child–Pugh classification, and tumor size (within 10%).ResultsTumor‐free survival rates were 45% and 48% at 3 years and 27% and 27% at 5 years in patients with and without diabetes, respectively. No significant difference was observed in the tumor‐free survival rates between patients with and without diabetes. Tumor‐free survival rates were 66% and 27% at 3 years in patients with normal postoperative glycated hemoglobin (HbA1c) level (HbA1c, <6.5%) and elevated postoperative HbA1c level (HbA1c, ≥6.5%), respectively. Multivariate analysis showed that poor glycemic control (HbA1c, ≥6.5%) was associated with postoperative tumor recurrence in patients with diabetes [odds ratio (OR) = 3.551, 95% confidence interval (CI) = 1.129–11.172, P = 0.030].ConclusionsCareful control of plasma glucose should be performed to prevent tumor recurrence after curative resection for HCV‐related HCC in patients with diabetes. J. Surg. Oncol. 2012; 105:606–611. © 2011 Wiley Periodicals, Inc.
Temporal and spatial expression of high‐mobility group box 1 in surgically injured rat vocal folds
Authors: Li Nicole Y.K. , Lee Byung‐Joo , Thibeault Susan L. 
Published: November 2011

AbstractObjectives/Hypothesis:High‐mobility group box 1 (HMGB1) protein has been identified as a principal instigator of injury‐induced inflammation in many organ systems. Physiologically, HMGB1 binds to chromatin in cell nucleus. Upon injury, cells release HMGB1 to extracellular milieu, triggering a destructive inflammatory response. Neutralizing or removing HMGB1 has been shown to control inflammation. Unfortunately, the role of HMGB1 in laryngeal inflammation and healing has yet to be defined. The purpose of this study was to determine spatial and temporal patterns of HMGB1 expression in surgically injured rat vocal folds up to 2 weeks after injury.Study Design:Prospective animal study.Methods:Bilateral vocal fold injury was performed on 70 Sprague‐Dawley rats. An additional 14 rats served as uninjured controls. Animals were sacrificed at 1 day, 3 days, 5 days, 1 week, and 2 weeks following surgery. Immunohistochemistry staining and enzyme‐linked immunosorbent assay (ELISA) were performed to determine the spatial distribution and temporal expression, respectively, of HMGB1 in vocal fold tissue. Hematoxylin‐and‐eosin staining for cell counting was performed to evaluate cell infiltration.Results:Cell number peaked significantly 5 days after injury. HMGB1 was positively stained in the nuclear, cytoplasmic, and extracellular compartments from days 1 to 7 after injury, whereas a strict nuclear staining was observed in uninjured controls and week 2 animals. Staining results were corroborated by ELISA.Conclusions:Spatial and temporal changes of HMGB1 expression were shown in injured vocal fold tissue, indicating this protein may be one of the principal drivers of inflammation and healing response to surgical injury in the larynx.
Complications and legal outcomes of tonsillectomy malpractice claims
Authors: Stevenson Andrew N. , Myer Charles M. , Shuler Matthew D. , Singer Peter S. 
Published: November 2011

AbstractObjectives/Hypothesis:To review malpractice cases involving complications following tonsillectomy.Study Design:Retrospective analysis at a tertiary medical center of jury verdict reports within the LexisNexis (Dayton, OH) database submitted after tonsillectomy malpractice cases.Methods:The LexisNexis MEGA Jury Verdicts and Settlements database was reviewed from 1984 through 2010 for complications resulting from tonsillectomy. Data including year of case, surgical complication, injury, case result, and judgment awarded were collected and analyzed.Results:One hundred seventy‐eight reports met inclusion criteria and were reviewed. Postoperative bleeding was the most common complication (33.7%), followed by anoxic events (16.9%), and impaired function (15.7%). Patient death occurred in 40.4% of reports and was most frequently associated with postoperative bleeding (54.2%), followed by anoxic events (18.1%), and postoperative medication issues (16.7%). Monetary awards were available in 24.7% of reports. Anoxic event was noted to have the highest median award at $3,051,296, followed by postoperative medication at $950,000.Conclusions:Tonsillectomy carries a large amount of risk from a malpractice standpoint. Postoperative bleeding is the complication most commonly associated with malpractice claims, but may not carry the greatest overall risk from a patient care or monetary standpoint. Hypoxic and anoxic events, although less common, appear to carry more morbidity for the patient and are associated with greater settlements and judgments in malpractice claims. Tonsillectomy continues to carry a significant mortality risk, albeit infrequent, and a high level of vigilance should be employed to help reduce these risks.
Interrater reliability of endoscopic parameters following sinus surgery
Authors: Smith Timothy L. , Hwang Peter H. , Murr Andrew H. , Lavigne Francois , Koreck Andrea 
Published: November 2011

AbstractObjectives/Hypothesis:To determine the interrater reliability of a set of postoperative endoscopic scoring parameters in patients with chronic rhinosinusitis who have undergone endoscopic sinus surgery (ESS).Study Design:Prospective cohort with retrospective review.Methods:One hundred twenty video‐endoscopic evaluations in 20 subjects recorded at 14, 30, and 45 days after ESS were scored in real time by the clinical investigators who performed the endoscopies and recorded the videos and retrospectively by an independent panel of four sinus surgeons who were blinded to all information. The scoring parameters included categoric grading for adhesion formation and middle turbinate position and continuous grading (visual analog scale) for degree of inflammation and crusting. Interrater reliability of the panel members was assessed using the Fleiss kappa test, bias index and prevalence index for categoric data, and the Shrout‐Fleiss test for continuous data. The level of agreement between the panel and the real‐time clinical investigator was also assessed.Results:For categoric variables, strong agreement between raters on the panel was found for both middle turbinate position (kappa = 0.499, prevalence index = 0.925) and adhesions (kappa = 0.364, prevalence index = 0.829). For continuous data, good agreement between raters was found for both inflammation (reliability coefficient = 0.554) and crusting (reliability coefficient = 0.620). Real‐time investigator scoring and panel scoring showed strong agreement.Conclusions:These results suggest that the endoscopic scoring parameters assessed (middle turbinate position, adhesions, inflammation, and crusting) have acceptable interexaminer reproducibility and are suitable for evaluating ESS outcomes in the postsurgical period.
Modified hemi‐lothrop procedure for supraorbital ethmoid lesion access
Authors: Friedel Mark E. , Li Shawn , Langer Paul D. , Liu James K. , Eloy Jean Anderson 
Published: November 2011

AbstractThe supraorbital ethmoid (SOE) cell is an accessory ethmoid cell in the frontal area that extends into and pneumatizes superolaterally along the orbital plate of the frontal bone. The outflow pathway of the SOE cell can become obstructed, leading to an SOE mucocele. Given their lateral location, SOE lesions are traditionally treated through external approaches, although some authors have advocated treatment through standard endoscopic routes. We present a case of a large, supraorbital ethmoid mucocele treated with a novel modified hemi‐Lothrop procedure (MHLP). This technique provides the benefit of an angulated approach to increase lateral visualization and bimanual, binostril instrumentation through a superior septectomy window.
National trends in oropharyngeal cancer surgery and the effect of surgeon and hospital volume on short‐term outcomes and cost of care
Authors: Gourin Christine G. , Frick Kevin D. 
Published: November 2011

AbstractObjectives/Hypothesis:The past 2 decades have witnessed an increase in the use of chemoradiation in the treatment of oropharyngeal cancer. We sought to characterize contemporary patterns of oropharyngeal cancer surgical care and the effect of volume on surgical care and short‐term outcomes.Study Design:Retrospective cross‐sectional study.Methods:Using the Nationwide Inpatient Sample database, temporal trends in oropharyngeal cancer surgical care were evaluated in 75,828 cases performed from 1993 through 2008, and relationships between volume and mortality, complications, length of stay, and costs were evaluated in 29,030 cases performed from 2003 through 2008 using regression analysis, adjusting for patient and provider characteristics.Results:Oropharyngeal cancer surgery from 2001 through 2008 was associated with increased use of high‐volume hospitals (odds ratio [OR], 2.2; P = .016); an increase in tonsil (OR, 1.4; P < .001) and tongue base (OR, 1.3; P = .001) tumors; an increase in patients aged 40 to 64 years (OR, 1.5; P < .001); a decrease in partial glossectomy (OR, 0.8; P < .001), pharyngectomy (OR, 0.8; P = .003), mandibulectomy (OR, 0.6; P < .001) and laryngectomy procedures (OR, 0.6; P < .001); and an increase in prior radiation (OR, 1.7; P = .026), advanced comorbidity (OR, 1.4; P = .007), and wound complications (OR, 1.5; P = .029), compared to 1993 through 2000. High‐volume hospitals were significantly associated with pharyngectomy (OR, 2.6; P = .001), while high‐volume surgeons were associated with flap reconstruction (OR, 1.6; P = .005) and prior radiation (OR, 2.6; P = .013). After controlling for all other variables, a statistically significant interaction was observed between high‐volume surgeons and high‐volume hospitals, with reduced hospital‐related costs for surgery performed by high‐volume surgeons at high‐volume hospitals.Conclusions:These data reflect changing trends in the epidemiology and primary management of oropharyngeal cancer, with meaningful differences in the type of surgical care provided by high‐volume providers.
Excess dietary iodine differentially affects thyroid gene expression in diabetes, thyroiditis‐prone versus ‐resistant BioBreeding (BB) rats
Authors: Swist Eleonora , Chen QiXuan , Qiao Cunye , Caldwell Don , Gruber Heidi , Scoggan Kylie A. 
Published: November 2011

AbstractScope: To identify genes involved in the susceptibility to iodine‐induced autoimmune thyroiditis.Methods and results: Diabetes, thyroiditis‐prone (BBdp) and ‐resistant (BBc) rats were fed either a control or a high‐iodine diet for 9 wk. Excess iodine intake increased the incidence of insulitis and thyroiditis in BBdp rats. BBdp rats fed the high‐iodine diet that did not develop thyroiditis had higher mRNA levels of Fabp4, Cidec, perilipin, Pparγ and Slc36a2 than BBdp rats fed the control diet and BBc rats fed either the control or the high‐iodine diet. BBdp rats fed the high‐iodine diet that did develop thyroiditis had higher mRNA levels of Cidec, Icam1, Ifitm1, and Slpi than BBdp rats fed the control diet and BBc rats fed either the control or the high‐iodine diet. BBdp rats that did develop thyroiditis had lower mRNA levels of Fabp4, perilipin and Slc36a2 but higher mRNA levels of Icam1, Ifitm1 and Slpi than BBdp that did not develop thyroiditis. Excess dietary iodine also increased the protein levels of Fabp4, Cidec and perilipin in BBdp rats.Conclusion: Differential expression of thyroid genes in BBdp versus BBc rats caused by excess dietary iodine may be implicated in autoimmune thyroiditis and insulitis pathogenesis.