bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimizing Esthetic Outcomes and Bone Stability: Chin Advancement Through Inverted V Osteotomy
Authors: Kang Kyo Joon , Kim Kenneth K. , Han Jinho , Park Bumjin 
Published: May 2024

Traditional horizontal osteotomies for small and short chins often yield suboptimal results due to limited bone advancement, resulting in deep labiomental folds and heightened bone resorption risks. This study investigates the effectiveness of an innovative inverted V-shaped osteotomy technique in enhancing esthetic outcomes for patients with such chin concerns. Thirty-eight patients who underwent inverted V-shaped osteotomy for recessed chins between January 2018 and June 2022 were included. Excluding cases involving simultaneous mandibular contouring surgery, patients were followed up for a median duration of 1.2±0.5 years. Preoperation and postoperation soft tissue pogonion (Pg’) and labiomental fold depth (LMF) changes were measured. IBM SPSS (version 27.0) was used for statistical analysis, with significance defined as P<0.05. Patient satisfaction was assessed using a visual analog scale. Successful advancement genioplasty was performed on all patients without any severe complications. The average change in soft tissue pogonion (Pg’) measured 6.2 (1.9) mm, and the mean alteration in labiomental depth was 0.42 (0.4) mm. The procedure achieved a bone to soft tissue movement ratio of 1:0.96. Patient satisfaction was notably high, with a mean VAS score of 8.7. An inverted V-shaped osteotomy enables greater bone advancement for small and short chins, leading to improved esthetic outcomes and offering a mechanically advantageous condition for bone segments.
Percutaneous Endoscopy and Image-guided Retrieval of Dropped Gallstones – A Case Series
Authors: Husnain Ali , Reiland Allison , Nemcek Albert A. , Salem Riad , Nagle Alexander P. , Teitelbaum Ezra , Riaz Ahsun 
Published: May 2024

Background:
Recurrent abscesses can happen due to dropped gallstones (DGs) after laparoscopic cholecystectomy (LC). Recognition and appropriate percutaneous endoscopy and image-guided treatment options can decrease morbidity associated with this condition.

Materials and Methods:
We report a minimally invasive endoscopy and image-guided technique for retrieval of dropped gallstones in a series of 6 patients (M/F=3/3; median age: 75.5 years [68 to 82]) presenting with recurrent or chronic intra-abdominal abscesses secondary to dropped gallstones. Technical success was defined as the visualization and retrieval of all stones. DGs were identified on pre-procedure imaging. Number of abscesses recurrence was 12 (1/6), 1 (3/6), and 0 (2/6) with a median interval of 2 months (1 to 21) between cholecystectomy and abscess development.

Results:
Percutaneous endoscopy and fluoroscopy guidance were utilized in all cases. Technical success was achieved in 4 patients (66%). The median procedure time was 65.8 minutes (39 to 136). The median fluoroscopy time and dose were 12.6 min (3.3 to 67) and 234 mGy (31 to 1457), respectively. There were no intraprocedure and postprocedure complications. No abscess recurrence was reported among successful procedures during a median follow-up of 193 days (51 to 308).

Conclusion:
Percutaneous image and endoscopy-guided lithotripsy/lithectomy are safe and effective. This technique is a suitable alternative to open surgery for dropped gallstones.

Level of Evidence:
Level 4, Case Series
Improving malignant fungating wound management among oncology nurses: a best practice implementation project
Authors: Chang Shing-Li , Chung Chi Feng , Liou Yueh Guo , Lo Shu Fen , Hu Sophia H. 
Published: May 2024

ABSTRACT

Introduction:
Appropriate malignant fungating wound (MFW) care is challenging for oncology nurses, leading to increased stress, compromised care quality, and poor patient outcomes.

Objective:
This study aimed to address best practice barriers and develop evidence-based guidelines for MFW care.

Methods:
This project was guided by the JBI Evidence Implementation Framework, which follows a seven-phase process. Both nurses’ skills and patient charts were audited to determine compliance with best practices for comprehensive MFW assessment, wound photo records, use of validated wound assessment tools, appropriate wound care, and patient pain and satisfaction. Bandura's social learning theory was used to guide the development of an online education program and an objective structured clinical examination for skill improvement to prompt behavior change in nurses. A follow-up audit was conducted to measure improvements in knowledge, skills, and self-efficacy among nurses to validate the effectiveness of the intervention.

Results:
The project resulted in improvements in all four evidence-based practice criteria: (1) comprehensive MFW assessments increased from 27% to 98%; (2) the inclusion of wound photos in medical records increased from 50% to 100%; (3) use of a validated wound assessment tool increased from 0% to 100%; and (4) appropriate interventions to manage wounds and maintain patients’ quality of life increased from 50% to 90%.

Conclusions:
The project integrated a flexible education program, multidisciplinary collaboration, and leadership support to empower nurses to effectively manage MFWs. In addition, Bandura's social learning theory was used to influence nurses’ behavior and bring about sustainable changes to organizational culture and practices.

Spanish abstract:

http://links.lww.com/IJEBH/A205
Variability in reported midpoints of (in)activation of cardiac INa
Authors: Clerx Michael , Volders Paul G.A. , Mirams Gary R. 
Published: May 2024

AbstractElectrically active cells like cardiomyocytes show variability in their size, shape, and electrical activity. But should we expect variability in the properties of their ionic currents? In this brief review we gather and visualise measurements of two important electrophysiological parameters: the midpoints of activation and inactivation of the cardiac fast sodium current, INa. We find a considerable variation in reported mean values between experiments, with a smaller cell-to-cell variation within experiments. We show how the between-experiment variability can be decomposed into a correlated and an uncorrelated component, and that the correlated component is much larger and affects both midpoints almost equally. We then review biological and methodological issues that might explain the observed variability, and attempt to classify each as within-experiment or correlated and uncorrelated between-experiment factors. Although the existence of some variability in measurements of ionic currents is well-known, we believe that this is the first work to systematically review it and that the scale of the observed variability is much larger than commonly appreciated, which has implications for modelling and experimental design.
Spatially Resolved Tumor Ecosystems and Cell States in Gastric Adenocarcinoma Progression and Evolution
Authors: Ma Haoran , Srivastava Supriya , Ong Xuewen , Tay Su Ting , Xu Chang , Sheng Taotao , Ho Shamaine Wei Ting , Lian Benedict Shi Xiang , Huang Kie Kyon , Goh Yeek Teck , Joseph Craig Ryan Cecil , Lum Jeffrey Huey Yew , Tan Angie Lay Keng , Zhang Yanrong , NG Michelle Shu Wen , Zhu Feng , Zhao Joseph J , Teh Ming , Yeong Joe , Yong Wei Peng , So Jimmy Bok Yan , Sundar Raghav , Tan Patrick 
Published: May 2024

AbstractGastric cancer (GC) is a major cause of global cancer mortality with high heterogeneity levels. To explore geospatial interactions in tumor ecosystems, we integrated 1,563 spatial transcriptomic regions-of-interest (ROIs) with 152,423 single-cell expression profiles across 130 GC samples from 70 patients. We observed pervasive expression-based intratumor heterogeneity, recapitulating tumor progression through spatially localized and functionally ordered subgroups with specific immune microenvironments and immune checkpoint profiles. Evolutionary phylogenetic analysis revealed two different evolutionary trajectories (branched evolution and diaspora evolution) associated with distinct molecular subtypes, clinical prognoses, stromal neighborhoods includingVWF+ACKR1+ endothelial cells, and genetic drivers such asSOX9. Spatial analysis of tumor-stromal interfaces across multiple GCs highlighted new ecosystem states not attributable to mere tumor/stroma admixture, landmarked by increasedGREM1expression. Our results provide insights into how the cellular ecosystems of individual GCs are sculpted by tumor intrinsic and extrinsic selective pressures, culminating in individualized patient-specific cancer cartographies.
Lateral Flow Assay Sensitivity and Signal Enhancement via Laser µ-Machined Constrains in Nitrocellulose Membrane
Authors: Khatmi Gazy , Klinavičius Tomas , Simanavičius Martynas , Silimavičius Laimis , Tamulevičienė Asta , Rimkutė Agnė , Kučinskaitė-Kodzė Indrė , Gylys Gintautas , Tamulevičius Tomas 
Published: May 2024

AbstractMultiplex lateral flow assay (LFA) is a handful diagnostic technology that can identify severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and other common respiratory viruses in one strip, which can be tested at the point-of-care without the need for equipment or skilled personnel outside the laboratory. Although its simplicity and practicality make it an appealing solution, it remains a grand challenge to substantially enhance the colorimetric LFA sensitivity. The local flow rate constraints imposed in nitrocellulose (NC) membranes via a number of vertical femtosecond laser micromachined microchannels are important for prolonged specific binding interactions. Porous NC membrane surfaces were structured with different widths and densities μ-channels employing a second harmonic of the Yb:KGW femtosecond laser and sample XYZ translation over a microscope objective-focused laser beam. The influence of the microchannel parameters on the vertical wicking speed was evaluated from the video recordings. The obtained results indicated that μ-channel length, width, and density in NC membranes controllably increased the immunological reaction time between the analyte and the labeled antibody by 950%. Image analysis of the colorimetric indicators confirmed that the flow rate delaying strategy enhanced the signal sensitives by 40% compared with pristine NC LFA.Graphical abstract
The universal labeling and sorting of avian primordial germ cell utilizingLycopersicon Esculentumlectin
Authors: Iikawa Hiroko , Morita Mizuki , Nishina Aika , Atsuta Yuji , Hayashi Yoshiki , Saito Daisuke 
Published: May 2024

SUMMARYAvian species serve as vital resources in human society, with the preservation and utilization of these species heavily reliant on primordial germ cells (PGCs). However, methods for effectively isolating these rare cells alive from embryos remain elusive in avian species beyond chickens. Even within chicken species, existing techniques have shown limited efficiency. In our study, we present a rapid, simple, and cost-effective method for labeling and sorting circulating-stage PGCs across various avian species, including Carinatae and Ratitae, utilizingLycopersicon Esculentumlectin. Significantly, this method demonstrates high sorting efficiency by identifying a wide range of PGC subtypes while preserving the proliferative potential of chicken PGCs. This approach is anticipated to make a significant contribution to the conservation, research, and agricultural industries related to birds worldwide.
SYNY: a pipeline to investigate and visualize collinearity between genomes
Authors: Julian Alexander Thomas , Pombert Jean-François 
Published: May 2024

AbstractInvestigating collinearity between chromosomes is often used in comparative genomics to help identify gene orthologs, pinpoint genes that might have been overlooked as part of annotation processes and/or perform various evolutionary inferences. Collinear segments, also known as syntenic blocks, can be inferred from sequence alignments and/or from the identification of genes arrayed in the same order and relative orientations between investigated genomes. To help perform these analyses and assess their outcomes, we built a simple pipeline called SYNY (for synteny) that implements the two distinct approaches and produces different visualizations. The SYNY pipeline was built with ease of use in mind and runs on modest hardware. The pipeline is written in Perl and Python and is available on GitHub (https://github.com/PombertLab/SYNY) under the permissive MIT license.
Y chromosome shredding inAnopheles gambiae: insight into the cellular dynamics of a novel synthetic sex ratio distorter
Authors: Vitale Matteo , Kranjc Nace , Leigh Jessica , Kyrou Kyrous , Courty Thomas , Marston Louise , Grilli Silvia , Crisanti Andrea , Bernardini Federica 
Published: May 2024

AbstractDespite efforts to explore the genome of the malaria vectorAnopheles gambiae, the Y chromosome of this species remains enigmatic. The large number of repetitive and heterochromatic DNA sequences makes the Y chromosome exceptionally difficult to fully assemble, hampering the progress of gene editing techniques and functional studies for this chromosome. In this study, we made use of a bioinformatic platform to identify Y-specific repetitive DNA sequences that served as a target site for a CRISPR/Cas9 system. The activity of Cas9 in the reproductive organs of males caused damage to Y-bearing sperm without affecting their fertility, leading to a strong female bias in the progeny. Cytological investigation allowed us to identify meiotic defects and investigate sperm selection in this new synthetic sex ratio distorter system. In addition, alternative promoters enable us to target the Y chromosome in specific tissues and developmental stages of male mosquitoes, enabling studies that shed light on the role of this chromosome in male gametogenesis. This work paves the way for further insight into the poorly characterised Y chromosome ofAnopheles gambiae. Moreover, the sex distorter strain we have generated promises to be a valuable tool for the advancement of studies in the field of developmental biology, with the potential to support the progress of genetic strategies aimed at controlling malaria mosquitoes and other pest species.Author summaryGenetic elements known as sex ratio meiotic drive can manipulate the sex ratio of offspring, favouring the male or female sex. This fascinating phenomenon has inspired the development of synthetic sex ratio distorter systems in several organisms. In species where females and males harbour XX and XY sex chromosomes respectively, the X-chromosome can be ‘shredded’ during male gametogenesis, leading to the production of non-functional X-bearing sperm, while Y-bearing sperm are left intact and able to fertilise the eggs. These systems can produce offspring that are extremely biased towards males, which can be used as genetic tools to control harmful insect populations. In our study, we applied this molecular strategy to target the Y chromosome ofAnopheles gambiae. Our aim was to investigate the cellular consequences of the shredding of this chromosome, the impact on meiosis and sperm selection, and the potential to achieve strong female bias in the offspring. The outcome of this study enhances our understanding of the molecular and biological mechanisms behind synthetic sex-ratio distorters in Anopheles mosquitoes, which could inform the development of vector control strategies that target sex ratio. Additionally, we present a genetic sexing strain able to produce mostly females, providing a valuable genetic tool for fundamental studies on this deadly vector.
Parent’s Views on Access to Dental Care and the Interim Canadian Dental Benefit
Authors: Menon Anil , de Jesus Vivianne Cruz , Virtanen Jorma I. , Schroth Robert J. 
Published: May 2024

AbstractIntroductionThis study investigated parents’ perspectives on access to oral health care and the Interim Canada Dental Benefit (CDB). In the context of Canada’s national health insurance, which historically excluded dental care, the introduction of the Interim CDB in October 2022 represented a paradigm shift towards enhancing dental care accessibility for children under 12 years of age from lower-income families.MethodsThis study analyzed aggregate and de-identified data from the comprehensive online survey conducted by The Strategic Counsel for Health Canada, involving 2,203 parents from across Canada. The survey was administered in March of 2023. Paired/overlap t-test for means and paired/overlap z-test for percentages were performed, with statistical significance atp≤ 0.05.ResultsThe majority of participants expressed concerns regarding the costs (90.9%) and accessibility (80.9%) of dental care, indicated that regular dental visits for children is important (97.2%), and would take their children more frequently to dental appointments if had extra money (79.9%). Some of the barriers preventing regular dental visits for children included costs of service and transportation and lack of insurance. The majority of parents showed support for the Interim CDB (87%), with the greatest support coming from the provinces of Manitoba and Saskatchewan (90.4%).ConclusionThis research underscores the imperative for ongoing evaluation and policy refinement to ensure the CDCP effectively addresses the nuanced needs of Canadian families, fostering a more inclusive and accessible dental care system. Parents’ concerns regarding dental care and their support for the Interim CDB signal a clear mandate for improving program outreach and accessibility through the Canadian Dental Care Plan (CDCP).Knowledge Transfer StatementFindings from this study highlight the significant concern among parents regarding dental care affordability in Canada, reinforcing the necessity of programs like the Interim Canada Dental Benefit (CDB) and Canadian Dental Care Plan. High rates of support suggest a positive public reception of the CDB, which is crucial for policy intervention’s success. Concern about accessing dental services, despite the availability of the insurance, indicates ongoing barriers to dental care, suggesting areas for future policy refinements.
The impact of APOE ε4 in Alzheimer’s disease: a meta-analysis of voxel-based morphometry studies
Authors: Bailey Madison , Ilchovska Zlatomira Georgieva , Hosseini Akram A. , Jung JeYoung 
Published: May 2024

AbstractBackgroundAlzheimer’s disease (AD) is the most prevalent form of dementia, exerting substantial personal and societal impacts. The apolipoprotein E (APOE) ε4 allele is a known genetic factor that increases the risk of AD, contributing to more severe brain atrophy and exacerbated symptoms.PurposeWe aim to provide a comprehensive review of the impacts of the APOE ε4 allele on brain atrophy in AD and mild cognitive impairment (MCI) as a transitional stage of AD.MethodsWe performed a coordinate-based meta-analysis of voxel-based morphometry (VBM) studies to identify the patterns of grey matter atrophy in APOE ε4 carriers vs. non-carriers. We obtained coordinate-based structural magnetic resonance imaging (MRI) data for 1135 individuals from 12 studies on PubMed and Google Scholar that met our inclusion criteria.ResultsWe found significant atrophy in the hippocampus and parahippocampus of APOE ε4 carriers compared to non-carriers, especially within the AD and MCI groups, while healthy controls showed no significant atrophy in these regions.ConclusionOur meta-analysis sheds light on the significant link between the APOE ε4 allele and hippocampal atrophy in both AD and MCI, emphasizing the allele’s critical influence on neurodegeneration, especially in the hippocampus. Our findings contribute to the understanding of the disease’s pathology, potentially facilitating progress in early detection, targeted interventions, and personalized care strategies for individuals with the APOE ε4 allele who are at risk for Alzheimer’s Disease.
Low-Frequency Activity in Dorsal Subthalamic Nucleus Predicts Impulsivity Improvement Following Deep Brain Stimulation in Parkinsonian Patients
Authors: Kaymak Ahmet , Cubeddu Laura , Vissani Matteo , Taddeini Fabio , Caremani Luca , Govoni Alessandra , Micheli Federico , Valente Simone , Piattellini Francesca , Greco Davide , Pecchioli Guido , Ramat Silvia , Mazzoni Alberto 
Published: May 2024

ABSTRACTObjectiveThe progression of impulsive-compulsive behaviors (ICB) in Parkinson’s Disease (PD) following subthalamic deep brain stimulation (DBS) surgery displays a large inter-patient variability. However, the link between the subthalamic neural activity at the single-neuron level and the postoperative evolution of ICB remains unclear. In this study, we investigated neural features associated with postoperative ICB recovery and their spatial distribution within the subthalamic nucleus (STN).ApproachWe examined neural activity extracted from intraoperative microelectrode recordings within the STN of 22 PD patients undergoing STN-DBS. Ten patients were diagnosed with ICB, with half of them showing recovery (ICB-R) from impulsive symptoms following implantation, while the other half remained stable (ICB-S). Both groups presented similar motor symptoms and received similar drug treatments pre- and post-operatively. Following, we compared beta [12-30 Hz] and theta [4-8 Hz] oscillations, firing rate, regularity, and spiking patterns in non-ICB, recovered, and stable patients across STN regions. We adopted linear discriminant algorithms to classify the postoperative state at both single neuron and patient levels.Main resultsWe observed significantly weaker beta and theta oscillations and increased spiking regularity at the single neuron level (p<0.05, Mann-Whitney U test) in patients who displayed postoperative ICB recovery. Of note, this difference was significant only on the dorsal portion of the STN, close to the stimulation target region. The discrimination algorithms based on these features correctly classified the postoperative state of 9/10 ICB patients.SignificanceWe showed that low-frequency subthalamic neural activity next to the stimulation target could be an effective biomarker for the evolution of ICBs following STN-DBS surgery, independently from other clinical aspects. Our results also support broader implications of beta activity in PD pathology beyond the motor domain.