bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimizing the connection of CRRT and ECMO lines with additional pressure regulator on the therapeutic effect, filter life, and incidence of complications
Authors: Huang Xihua , Gao Yongxia , Chen Xufeng , Mei Yong , Zhang Hui , Tian Yali , Wu Juan 
Published: June 2024

Background:
Extracorporeal membrane oxygenation (ECMO) is used for severe cardiopulmonary failure, with veno-arterial ECMO for cardiogenic shock and veno-venous ECMO for acute respiratory failure. ECMO’s application has expanded to ICUs, emergency departments, and operating rooms. ECMO patients are at high risk for complications, including acute kidney injury (AKI), often requiring renal replacement therapy (RRT), posing significant management challenges.

Methods:
From August 2015 to June 2022, 120 patients were cured with veno-venous ECMO (n = 60) or veno-arterial ECMO (VA-ECMO, n = 60) combined with CRRT in our hospital. In the control group (n = 60), the input end (arterial end) of CRRT was connected to the ECMO oxygenator. The reinfusion end (venous end) of CRRT was connected to the oxygenator of ECMO for CRRT + ECMO treatment. In the experimental group (n = 60), the input end (arterial end) of CRRT was connected to the oxygenator of ECMO, and an additional pressure regulating device was installed on the connection of the 2 lines. The observation indexes including clinical therapeutic effect, clinical therapeutic effect, the incidence of complications, and the incidence of complications were compared.

Results:
There was a notable decrease in serum creatinine, and the differences in blood urea nitrogen, procalcitonin, and C-reactive protein after operation were statistically significant (P < .05). The filter use time in the study group was notably longer (P < .01). There exhibited no remarkable difference in the incidences of bleeding, thrombosis, numbness of hands and feet, metabolic alkalosis, disseminated intravascular coagulation, organ dysfunction syndrome, hyperbilirubinemia, and infection.

Conclusion:
This study demonstrates that additional pressure regulation devices are installed at the line connection between the CRRT input end and the CRRT return end to ensure that the flow rate of ECMO does not affect the CRRT treatment. ECMO and CRRT provide a safe pressure range so that the ECMO line can be safely connected to the CRRT machine at physiological pressure, reducing the occurrence of complications related to CRRT machine interruption and improving the efficiency of CRRT without affecting the efficiency of ECMO, ensuring patient safety.
Effectiveness and safety of motion-style acupuncture treatment using traction for inpatients with acute low back pain caused by a traffic accident: A randomized controlled trial
Authors: Park Byung-Hak , Han Jeong-Hun , Park Jin-Hun , Min Tae-Woon , Lee Hyun-Jun , Lee Yoon Jae , Lee Sook-Hyun , Park Kyoung Sun , Ha In-Hyuk 
Published: June 2024

Background:
Musculoskeletal symptoms, such as neck pain and low back pain (LBP) are common after a traffic accident (TA). While motion-style acupuncture treatment (MSAT) is effective in relieving pain, MSAT using traction (T-MSAT) has rarely been studied, and evidence for its efficacy and safety is lacking. To address this gap, this study aimed to assess the effectiveness and safety of T-MSAT for pain and functional disturbances in patients with acute LBP caused by a TA.

Methods:
This two-armed, parallel, assessor blinded randomized controlled trial, conducted at Jaseng Hospital of Korean Medicine, included 100 patients with acute LBP occurring within 1 week of a TA. The participants were randomly allocated (1:1 ratio) to receive either combined T-MSAT and integrative Korean medicine treatment (IKMT) or only conventional IKMT, applied for 3 consecutive days after admission. The primary outcome was the difference between numerical rating scale (NRS) scores for LBP at baseline and after treatment completion on day 4 after admission.

Results:
At the primary endpoint, the difference in NRS scores for LBP between the T-MSAT and control groups was 0.94 (95% confidence interval [CI] 0.40–1.48). The T-MSAT group showed a significantly lower NRS score for LBP than the control group. Differences in visual analogue scale (VAS) scores between the T-MSAT and control groups were significant at baseline and discharge. The area under the curve of the VAS score showed a significant difference (−46.86 [95% CI −85.13 to −8.59]), indicating faster pain reduction in the T-MSAT group than in the control group. Recovery (30% pain reduction) was achieved more rapidly in the T-MSAT group than in the control group (log-rank test P = .005). Meanwhile, the NRS, VAS, Oswestry disability index, and quality of life scores at discharge or at the 12-week follow-up showed no significant difference. The rates of mild adverse events (AEs) were comparable between the groups. No severe AEs were reported, and none of the AEs were associated with the clinical trial.

Conclusions:
T-MSAT combined with IKMT is a safe treatment that can effectively and quickly reduce initial pain in patients with LBP.
Relationship between salusin beta levels and the severity of acute pancreatitis in patients
Authors: Albayrak Bulent , Guneyin Emel Ayvaz , Celik Muhammet 
Published: June 2024

Background:
This study aimed to evaluate the significance of serum salusin beta (SAL-β) levels in predicting the severity of acute pancreatitis (AP) in patients diagnosed with this condition and to assess its relationship with disease and prognosis.

Methods:
Sixty-four patients between 18 and 100 years of age diagnosed with AP, were included in the study. Patients were categorized into 3 groups based on the Revised Atlanta Classification: mild, moderate, and severe AP. Eighteen healthy adults were included as the control group. Sex, age, height, weight, presence of additional diseases, laboratory results, imaging findings, levels of white blood cells, neutrophil-lymphocyte ratio, mean platelet volume, amylase, lipase, sensitive C-reactive protein, sedimentation, and serum SAL-β were measured and recorded. SAL-β levels were reevaluated on the third day of hospitalization.

Results:
The average age of the patients included in the study was 62.66 ± 17.67. Gallstones were present in 64.1% of the patients. The difference in the SAL-β averages on the 1st and 3rd days was statistically significant (P < .05). On the first day, the SAL-β averages of those with severe Atlanta scores were higher than those with mild and moderate Atlanta severity. Similarly, on the third day, the SAL-β averages of those with severe Atlanta scores were higher than those with mild and moderate Atlanta severity. According to receiver operating characteristic analysis using the Youden index, the cutoff value for SAL-β for severe pancreatitis was 178.8 pg/mL on the 1st day and 207.5 pg/mL on the 3rd day.

Conclusion:
SAL-β can be used to detect and monitor severe pancreatitis. Further extensive clinical studies with larger case series are needed.
Giant Cyst of Intrapancreatic Accessory Spleen Mimicking a Malignant Cystic Neoplasm of the Pancreas
Authors: Sun Yang , Luo Jieli , Huang Fengbo , Huang Pintong , Yu Risheng 
Published: June 2024

Introduction
Epidermoid cyst in an intrapancreatic accessory spleen (ECIPAS) is an exceedingly rare pancreatic lesion that is always mistakenly suspected of malignancy preoperatively.

Case summary
A 25-year-old male patient incidentally found a giant mass in the left upper abdomen neighboring the hilum of the spleen. The patient denied any obvious discomfort. Except for a slightly elevated carbohydrate antigen 19-9 (CA-19-9, 43.5 U/mL), no abnormal laboratory test results were found. Contrast-enhanced computed tomography, conventional ultrasound, and magnetic resonance imaging were performed. The patient received a laparoscopic distal pancreatectomy. The final pathology showed ECIPAS. The postoperative course was uneventful and no signs of recurrence during 2 years of follow-up.

Discussion
For an incidental pancreatic cystic lesion, ECIPAS should be considered in the differential diagnosis. ECIPAS may mimic pancreatic cystadenoma. Imaging follow-up or surgical removal may be useful for the exclusion of malignant risks in ECIPAS.
Longitudinal controlled attenuation parameter and liver stiffness in children with and without perinatal HIV infection in South Africa
Authors: Rose Penelope C. , Davies Claire , Cotton Mark F. , Otwombe Kennedy , Browne Sara H. , Vaida Florin , Innes Steve , Nel Etienne De la Rey 
Published: June 2024

Objectives:
Metabolic dysfunction-associated steatotic liver disease (MASLD) is an emerging cause of liver disease in HIV. Transient elastography (TE) with controlled attenuation parameter (CAP) measures liver stiffness as a marker of liver fibrosis and CAP as a measure of hepatic steatosis. Our aim was to evaluate longitudinal CAP and liver stiffness in children with perinatally acquired HIV (PHIV) on antiretroviral therapy (ART) from early life compared to children without HIV (HU).

Design:
Prospective cohort study.

Methods:
PHIV and HU were followed annually for two years. During the study, 60% of PHIV switched from older ART regimens to tenofovir disoproxil, lamivudine and dolutegravir (TLD). Longitudinal evolution of CAP and liver stiffness were investigated in two PHIV groups − on older ART and on TLD − compared to HU children using linear mixed effects models.

Results:
263 children and adolescents (112 PHIV, 151 HU) aged 7–20 years were followed. PHIV on older ART had CAP 8.61% (95% CI 4.42–12.97, P < 0.001) greater than HU and no significant difference in CAP between PHIV on TLD and HU. No significant difference in liver stiffness was found between PHIV on older ART regimens and PHIV on TLD compared to HU.

Conclusion:
PHIV on older ART had higher CAP than HU, whereas in PHIV switched to TLD there was no difference in CAP compared to HU. There was no difference in liver stiffness between either PHIV group and HU. This suggests starting ART early in life might protect PHIV from developing hepatic fibrosis.
Evaluating Islet Cell Isolation and Transplantation From Donors Following Medical Assistance in Dying
Authors: Parente Alessandro , Verhoeff Kevin , Kin Tatsuya , Hefler Joshua , Marfil-Garza Braulio A. , Sanchez-Fernandez Norberto , Lam Anna , Lyon James , O’Gorman Doug , Dajani Khaled Z. , Anderson Blaire L. , Bigam David L. , MacDonald Patrick E. , Shapiro A. M. James 
Published: June 2024

Background.
Limited information is available regarding outcomes of islet cell isolation (ICI) and transplantation (ITx) using medical assistance in dying (MAiD) donors. We aimed to assess the feasibility and outcomes of ICI and ITx in MAiD donors.

Methods.
ICI and ITx from MAiD were compared with donation after circulatory death (DCD) type III between 2016 and 2023. Differences of isolated islet equivalents (IEQs), numeric viability and other quantitative in vitro metabolic measures were assessed.

Results.
Overall, 81 ICIs were available of whom 34 (42%) and 47 (58%) from MAiD and DCD-III, respectively. There were no differences of pancreas and digested tissue weight and islets viability among the 2 groups; however, cold ischemic time was longer in MAiD (11.5 versus 9.1 h; P = 0.021). The IEQ (P < 0.001) and percent trapped (P < 0.001) were higher in the DCD-III; however, MAiD islets demonstrated a higher purity (P = 0.020). Overall, 15 ITx were performed of whom 3 (8.8%) and 12 (25.5%) from MAiD and DCD-III, respectively (P = 0.056). Patients had a median fasting C-peptide of 0.51 ng/mL (interquartile range, 0.30–0.76 nmol/L), with no differences between groups (MAiD = 0.52 versus DCD-III = 0.51; P = 0.718). The median HbA1c was 6.2% (interquartile range, 5.7%–7%) (MAiD = 6.3% versus DCD-III = 6.1%; P = 0.815) and BETA2 scores (MAiD = 7.4 versus DCD-III = 12.8; P = 0.229) did not differ.

Conclusions.
ICI from MAiD donor pancreas may be successfully transplanted with comparable outcomes to DCD-III and may be used for research. These results justify additional efforts to consider MAiD as another valuable source of grafts for ITx. Further multicenter studies and larger clinical experience are needed to validate our findings.
Reverse engineering neuron type-specific and type-orthogonal splicing-regulatory networks using single-cell transcriptomes
Authors: Moakley Daniel F , Campbell Melissa , Anglada-Girotto Miquel , Feng Huijuan , Califano Andrea , Au Edmund , Zhang Chaolin 
Published: June 2024

AbstractCell type-specific alternative splicing (AS) enables differential gene isoform expression between diverse neuron types with distinct identities and functions. Current studies linking individual RNA-binding proteins (RBPs) to AS in a few neuron types underscore the need for holistic modeling. Here, we use network reverse engineering to derive a map of the neuron type-specific AS regulatory landscape from 133 mouse neocortical cell types defined by single-cell transcriptomes. This approach reliably inferred the regulons of 350 RBPs and their cell type-specific activities. Our analysis revealed driving factors delineating neuronal identities, among which we validated Elavl2 as a key RBP for MGE-specific splicing in GABAergic interneurons using an in vitro ESC differentiation system. We also identified a module of exons and candidate regulators specific for long- and short-projection neurons across multiple neuronal classes. This study provides a resource for elucidating splicing regulatory programs that drive neuronal molecular diversity, including those that do not align with gene expression-based classifications.
Keetia korupensis sp. nov. (Rubiaceae - Vanguerieae) a threatened lowland evergreen forest climber and the endemic plant species of Korup National Park, SW Region, Cameroon
Authors: Cheek Martin , Poundje Maguy , Pearce Laura 
Published: June 2024

SummaryA review of the strictly endemic plant species of the Korup National Park, S.W. Region, Cameroon, is presented together with consideration of the importance of Korup for global plant conservation. Eighteen strict endemic species are known, 14 of which occur in lowland evergreen forest habitat (includingKeetia korupensis) and of which 3 are among the 29 grove-forming monodominant detarioid legume species for which Korup is world famous. Four of Korup’s endemic species are rheophytes restricted to the Mana (Ndian) River rapids and falls which is probably the most species diverse site for shrubby rheophytes on the African continent. No endemics are yet known from Korup’s submontane forest, possibly because this is comparatively small in extent.Keetia korupensisCheek, a rainforest climber, is described and illustrated as strictly endemic, on current evidence, to the Korup National Park. The species differs from all other known species of the genus in its stipitate 2-seeded fruits which are twice as long as broad (vs non-stipitate and subisodiametric in most of the species) and for the 2 mm long stiff, skin-penetrating stem indumentum (vs non-injuring or soft in other long hairy species).Keetia korupensisis provisionally assessed using the IUCN standard as Vulnerable (VU D2).
Ecological and functional niches comparison reveals differentiated resource-use strategies and ecological thresholds in four key floating-leaved macrophytes
Authors: Dalla Vecchia Alice , Castellani Maria Beatrice , Azzella Mattia M. , Bolpagni Rossano 
Published: June 2024

ABSTRACTNiche theory has been widely used in ecology; however, few studies have attempted to combine information on functional and ecological niches (i.e. variation in traits and environmental requirements), especially for freshwater macrophytes.In this study we aim to describe the functional and ecological niches of four key nymphaeid species (Nuphar lutea,Nymphaea alba,Nelumbo nuciferaandNymphoides peltata) to investigate their environmental tolerance and functional adaptability.Twelve Italian populations per species were sampled. Functional and ecological niches were determined using hypervolumes based on eight functional traits and environmental variables, related to leaf structure and economics spectrum, and to water and sediment quality.Among the three Italian native species,N. luteaandN. albashowed intermediate niche size and position in the functional and ecological space, althoughN. albaappears to be less competitive due to a small functional niche and lower trait performance, which could explain its general tendency to decline.N. peltataappeared more specialized in its environmental requirements and characterized by highly acquisitive leaves, while the invasive alienN. nuciferaexerted its competitive success by distinguishing its functional niche and expanding its ecological niche, through high investment of resources in leaves.Overall, all four target species share similar ecological niches, colonizing eutrophic ecosystems typical of intensive agricultural landscapes, but show different patterns in their functional niche. We demonstrate the applicability of an approach based on both functional and ecological niches to unravel species’ adaptation and strategies.
On some alien species from Emilia-Romagna harbors (northern Adriatic Sea, Italy)
Authors: Lezzi M. , Mazziotti C. 
Published: June 2024

AbstractIncreased global trade has led to a surge in the introduction of non-indigenous species (NIS) worldwide. Ships, particularly merchant vessels and recreational yachts, act as vectors for these invasions by transporting NIS in ballast water or as hull fouling. This study investigates the introduction of new fouling NIS in the North Adriatic Sea. Our field research at the ports of Ravenna and Cesenatico identified several alien species, including new records for the North Adriatic Sea:Polyandrocarpa zorritensis, Aplidium accarenseandCelleporaria brunnea. Additionally, a significant presence of the cryptogenic speciesMolgula manhattensiswas observed in Cesenatico.
Embracing Uncertainty Reshapes the ETHYLENE INSENSITIVE2-Activated Ethylene Signaling Framework
Authors: Zhao Hangwei , Zhang Ying , Chen Yuying , Wang Chenrunshu , Liu Qian , Zhang Jingyi , Wen Chi-Kuang 
Published: June 2024

AbstractSignal transduction of the gaseous plant hormone ethylene by ETHYLENE INSENSITIVE2 (EIN2) is proposed to be regulated at multiple levels. EIN2 is phosphorylated by CONSTITUTIVE TRIPLE-RESPONSE1 (CTR1), subject to the F-Box proteins [EIN2-TARGETING PROTEIN1 (ETP1) and ETP2]-mediated degradation, and cannot activate ethylene signaling. Ethylene prevents EIN2 phosphorylation and degradation, and EIN2 accumulates and activates the signaling. MAOHUZI3 LIKE1 (MHL1) and MHL2 stabilize EIN2, and themhl1 mhl2double mutation confers ethylene insensitivity. Here, we reported that the regulation of EIN2-activated ethylene signaling can be independent of CTR1-mediated phosphorylation, ETP1/ETP2-directed degradation, and MHL1/MHL2-dependent stabilization. Both theetp1 etp2double mutant and wild type showed identical ethylene dose-response curves, and the nontreatedmhl1 mhl2double mutant displayed substantial growth inhibition. The reported ethylene-insensitive root phenotype ofmhl1 mhl2seedlings requires sucrose and is associated with unknown loci. The ethylene receptor ETHYLENE RESPONSE1 (ETR1) and EIN2 interact at the rough endoplasmic reticulum. We propose that the docking of CTR1 to ETR1 promotes receptor signaling, which inhibits EIN2-activated ethylene signaling. The discrepancy between our findings and the current model is discussed. Our findings may disrupt the knowledge boundary of the present molecular model, developing a niche for findings outside the framework to advance our understanding of ethylene signaling.
Plant miRNAs Anti-Staphylococcus aureus:Therapeutic Perspective
Authors: Gharsallah Charfeddine 
Published: June 2024

AbstractStaphylococcus aureusis a pathogen that has developed resistance to each new antibiotic introduced for half a century especially through the acquisition of themecAgene. This bacterial resistance to antibiotics represents a major public health problem. New revolutions are underway, in particular the design of drugs and vaccines targeting the system for detecting the regulatory quorum of the accessory gene (agr). It has been shown that the pathogenicity and resistance ofS. aureuscan be modulated through the intervention of this system. For this reason, we propose in this present work, a new therapeutic design based on anin silicostudy to identify plant miRNAs that could target this system as well as themecAgene based on recent studies showing the inter-realm regulation of human transcripts by plant miRNAs. Out of a total of 20643 miRNAs from mature plants, we identified 29 miRNAs, obtained by the selection criteria MFE ≥ -25 kcal / mol, which could potentially target selected genes ofS. aureus. Fifteen of them were selected on the basis of their thermodynamic stability. Interestingly, The seeds of ptc - miR171g and ptc - miR171h (Populus trichocarpa) both of which almost similar mature miRNA sequence was found to target UCCC region ofRNAIIIgene. miRNAs from plants targetingS. aureushave the potential to be developed as an alternative therapy of the future. To our knowledge, it is the first therapeutic alternative of the future via thein silicoidentification of plant miRNAs targeting pathogenic bacteria.Graphical Abstract
Lipid vesicle formation by encapsulation of SMALPs in surfactant-stabilised droplets
Authors: Waeterschoot Jorik , Barniol-Xicota Marta , Verhelst Steven , Baatsen Pieter , Koos Erin , Lammertyn Jeroen , Casadevall i Solvas Xavier 
Published: June 2024

AbstractUnderstanding the intricate functions of membrane proteins is pivotal in cell biology and drug discovery. The composition of the cell membrane is highly complex, with different types of membrane proteins and a huge variety of lipid species, Hence, studying cellular membranes in a complexity-reduced context is important to enhance our understanding of the roles of the different elements. However, reconstitution of membrane proteins in an environment that closely mimics the cell, like giant unilamellar vesicles (GUVs), remains challenging, often requiring detergents that compromise protein function. To address this challenge, we present a novel strategy to manufacture GUVs from styrene maleic acid lipid particles (SMALPs) that utilises surfactant-stabilised droplets as a template. Harnessing a new form of SMA linked to fluorescein, which we call FSMA, we demonstrate the assembly of SMALPs at the surfactant-stabilised droplet interface, resulting in the formation of GUVs when released upon addition of a demulsifying agent. The released vesicles appear similar to electroformed vesicles imaged with confocal light microscopy, but a fluorescein leakage assay and cryo-TEM imaging reveal their porous nature, potentially the result of residual interactions of SMA with the lipid bilayer. Our study represents a significant step towards opening new avenues for comprehensive protein research in a complexity-reduced, yet biologically relevant, setting.