bluuebunny/crossref_metadata_2025_split huggingface dataset:
IGF‐1 deficiency resists cardiac hypertrophy and myocardial contractile dysfunction: role of microRNA‐1 and microRNA‐133a
Authors: Hua Yinan , Zhang Yingmei , Ren Jun 
Published: March 2011

AbstractThis study was designed to examine the impact of insulin‐like growth factor‐1 (IGF‐1) deficiency on abdominal aortic constriction (AAC)‐induced cardiac geometric and functional changes with a focus on microRNA‐1, 133a and 208, which are specially expressed in hearts and govern cardiac hypertrophy and stress‐dependent cardiac growth. Liver‐specific IGF‐1‐deficient (LID) and C57/BL6 mice were subject to AAC. Echocardiographic and cardiomyocyte function were assessed 4 wks later. Haematoxylin and eosin staining was used to monitor myocardial morphology. Western blot and real‐time PCR were used to detect protein and miR expression, respectively. Neonatal rat cardiomyocytes (NRCMs) were transfected with miRs prior to IGF‐1 exposure to initiate cell proliferation. Immunohistochemistry and [3H] Leucine incorporation were used to detect cell surface area and protein abundance. C57 mice subject to AAC displayed increased ventricular wall thickness, decreased left ventricular end diastolic and end systolic dimensions and elevated cardiomyocyte shortening capacity, all of which were attenuated in LID mice. In addition, IGF‐1 deficiency mitigated AAC‐induced increase in atrial natriuretic factor, GATA binding protein 4, glucose transporter 4 (GLUT4) and Akt phosphorylation. In contrast, neither AAC treatment nor IGF‐1 deficiency affected glycogen synthase kinase 3b, mammalian target of rapamycin, the Glut‐4 translocation mediator Akt substrate of 160 kD (AS160) and protein phosphatase. Levels of miR‐1 and ‐133a (but not miR‐208) were significantly attenuated by AAC in C57 but not LID mice. Transfection of miR‐1 and ‐133a obliterated IGF‐1‐induced hypertrophic responses in NRCMs. Our data suggest that IGF‐1 deficiency retards AAC‐induced cardiac hypertrophic and contractile changes via alleviating down‐regulation of miR‐1 and miR‐133a in response to left ventricular pressure overload.
RETRACTED: Crestal bone loss evaluation in osseotite expanded platform implants: a 5‐year study
Authors: Calvo‐Guirado José Luis , Gómez‐Moreno Gerardo , López‐Marí Laura , Guardia Javier , Negri Bruno , Martínez‐González José María 
Published: March 2011

AbstractObjective: The aim of this prospective clinical study was to evaluate the survival rates at 5 years of expanded platform implants placed in the anterior zone of the maxilla and immediately restored with single crowns.Materials and methods: Implants incorporating the platform‐switching concept were placed in fresh extraction sockets in the maxillary arch, with each patient receiving a provisional restoration immediately after implant placement. After 15 days, final screwed restorations were inserted. Mesial and distal bone heights were evaluated using digital radiography on the day following implant placement and at 1, 3, 6, 9, 12, 24, 36 months and 5 years. Primary stability was measured with resonance frequency analysis (RFA) using the Osstell Mentor device. Sixty‐four implants were placed in 32 men and 32 women ranging in age between 29 and 60 (mean: 39.64 ± 5.16 years).Results: Mean mesial bone loss was 0.08 mm (SD 0.42). Mean distal bone loss was 0.14 mm (SD 0.56). Over the course of the 5 years, the mean RFA value was 72.5 ± 3.1 SD.Conclusion: The platform‐switched implants remained stable over the course of 5 years and had an overall survival rate of 97.1%.To cite this article:
Calvo‐Guirado J L, Gómez‐Moreno G, López‐Marí L, Guardia J, Negri B, Martínez‐González J M. Crestal bone loss evaluation in osseotite expanded platform implants: a 5‐year study. Clin. Oral Impl. Res. xx, 2011; 000–000 doi: 10.1111/j.1600‐0501.2010.02130.x
The effect of combined delivery of recombinant human bone morphogenetic protein‐2 and recombinant human vascular endothelial growth factor 165 from biomimetic calcium‐phosphate‐coated implants on osseointegration
Authors: Ramazanoglu Mustafa , Lutz Rainer , Ergun Celaletdin , von Wilmowsky Cornelius , Nkenke Emeka , Schlegel Karl Andreas 
Published: March 2011

AbstractObjectives: The delivery of growth factors for enhanced osseointegration depends on the effectiveness of the carrier systems at the bone–implant interface. This study evaluated the effect of solo and dual delivery of recombinant human bone morphogenetic protein‐2 (rhBMP‐2) and recombinant human vascular endothelial growth factor (rhVEGF165) from biomimetically octacalcium phosphate‐coated implants on osseointegration.Materials and methods: Biomimetic implants, bearing either a single growth factor (BMP or VEGF) or their combination (BMP+VEGF), were established, and compared with acid‐etched (AE, control) and biomimetic implants without growth factor (CAP). Implants were placed into frontal skulls of nine domestic pigs. The quality of osseointegration was evaluated using microradiographic and histomorphometric analysis of bone formation inside four defined bone chambers of the experimental implant at 1, 2 and 4 weeks.Results: Biomimetic implants, either with or without growth factor, showed enhanced bone volume density (BVD) values after 2 and 4 weeks. This enhancement was significant for the BMP and BMP+VEGF group compared with the control AE group after 2 weeks (P<0.05). All biomimetic calcium‐phosphate (Ca‐P) coatings exhibited significantly enhanced bone–implant contact (BIC) rates compared with the uncoated control surface after 2 weeks (P<0.05). However, the combined delivery of BMP‐2 and VEGF did not significantly enhance BIC at the final observation period.Conclusion: It was concluded that the combined delivery of BMP‐2 and VEGF enhances BVD around implants, but not BIC. Therefore, it may be assumed that changes in the surface characteristics should be considered when designing growth factor‐delivering surfaces.To cite this article:
Ramazanoglu M, Lutz R, Ergun C, von Wilmowsky C, Nkenke E, Schlegel K A. The effect of combined delivery of recombinant human bone morphogenetic protein‐2 and recombinant human vascular endothelial growth factor 165 from biomimetic calcium‐phosphatecoated implants on osseointegration. Clin. Oral Impl. Res. xx, 2011; 000–000. doi: 10.1111/j.1600‐0501.2010.02133.x
Iron deficiency and Helicobacter pylori infection in children
Authors: Vendt N , Kool P , Teesalu K , Lillemäe K , Maaroos H‐I , Oona M 
Published: March 2011

AbstractAim:  To examine the relationship between iron deficiency (ID) and Helicobacter pylori infection in school‐aged children.Methods:  Altogether 363 children from ambulatory admission were consecutively enrolled in the study. Haemoglobin (Hb), soluble transferrin receptor (sTfR), IgG against H. pylori and IgA against tissue transglutaminase were measured. The criteria for ID were sTfR > 5.7 mg/L in children aged 7–12 years and sTfR > 4.5 mg/L in older children, for anaemia Hb < 115 g/L in the younger group and Hb < 130 g/L for older boys and Hb < 120 g/L for girls.Results:  Iron deficiency was found in 17% of the children, 5% had also anaemia. H. pylori colonization was detected in 27% and serum markers for coeliac disease in 0.6% of the children. The prevalence of ID and H. pylori seropositivity was higher in older children (23% and 29%, vs 9% and 22%, respectively). Children with H. pylori were significantly shorter [length SDS 1.0 (0.98–1.01) vs 0.98 (0.97–0.99)]. Older children had risk for ID (OR 1.1, 95% CI 1.0–1.3, p = 0.03). Although the prevalence of H. pylori seropositivity was higher in the ID group, it was not significantly associated with ID in multivariate analysis.Conclusion:  Helicobacter pylori seropositivity was not associated with ID. The associated factor for ID was age.
Substrate preference and phosphatidylinositol monophosphate inhibition of the catalytic domain of the Per‐Arnt‐Sim domain kinase PASKIN
Authors: Schläfli Philipp , Tröger Juliane , Eckhardt Katrin , Borter Emanuela , Spielmann Patrick , Wenger Roland H. 
Published: March 2011

The Per‐Arnt‐Sim (PAS) domain serine/threonine kinase PASKIN, or PAS kinase, links energy flux and protein synthesis in yeast, regulates glycogen synthesis and protein translation in mammals, and might be involved in insulin regulation in the pancreas. According to the current model, binding of a putative ligand to the PAS domain disinhibits the kinase domain, leading to PASKIN autophosphorylation and increased kinase activity. To date, only synthetic but no endogenous PASKIN ligands have been reported. In the present study, we identified a number of novel PASKIN kinase targets, including ribosomal protein S6. Together with our previous identification of eukaryotic elongation factor 1A1, this suggests a role for PASKIN in the regulation of mammalian protein translation. When searching for endogenous PASKIN ligands, we found that various phospholipids can bind PASKIN and stimulate its autophosphorylation. Interestingly, the strongest binding and autophosphorylation was achieved with monophosphorylated phosphatidylinositols. However, stimulated PASKIN autophosphorylation did not correlate with ribosomal protein S6 and eukaryotic elongation factor 1A1 target phosphorylation. Although autophosphorylation was enhanced by monophosphorylated phosphatidylinositols, di‐ and tri‐phosphorylated phosphatidylinositols inhibited autophosphorylation. By contrast, target phosphorylation was always inhibited, with the highest efficiency for di‐ and tri‐phosphorylated phosphatidylinositols. Because phosphatidylinositol monophosphates were found to interact with the kinase rather than with the PAS domain, these data suggest a multiligand regulation of PASKIN activity, including a still unknown PAS domain binding/activating ligand and kinase domain binding modulatory phosphatidylinositol phosphates.Structured digital abstract
A list of the large number of protein‐protein interactions described in this article is available via the MINT article IDMINT‐8145255
The role of binding energies of neutron stars on the accretion-driven evolution
Authors: Bagchi Manjari 
Published: March 2011

Abstract
Millisecond pulsars are believed to descend from low-mass X-ray binaries. Observable parameters of binary millisecond pulsars, e.g. mass of the pulsar, mass of the companion, spin period of the pulsar, orbital period, orbital eccentricity, etc., are used to probe the past accretion history of the millisecond pulsars. However, unfortunately in these studies the binding energy of the neutron star is not commonly considered. We show that the effect of the binding energy is significant in the estimation of the amount of mass accretion, and thus should be incorporated in models for binary evolutions. Moreover, different equations of state for dense matter give different values for the accreted mass for the same amount of increase in the gravitational mass of the neutron star, implying the need of constraining dense matter equations of state even to understand the spin-up procedure properly.
Are cold flows detectable with metal absorption lines?
Authors: Kimm Taysun , Slyz Adrianne , Devriendt Julien , Pichon Christophe 
Published: March 2011

Abstract
Cosmological simulations have shown that dark matter haloes are connected to each other by large-scale filamentary structures. Cold gas flowing within this ‘cosmic web’ is believed to be an important source of fuel for star formation at high redshift. However, the presence of such filamentary gas has never been observationally confirmed despite the fact that its covering fraction within massive haloes at high redshift is predicted to be significant (∼25 per cent). In this Letter, we investigate in detail whether such cold gas is detectable using low-ionization metal absorption lines, such as Ciiλ1334, as this technique has a proven observational record for detecting gaseous structures. Using a large statistical sample of galaxies from the MareNostrumN-body+ adaptive mesh refinement (AMR) cosmological simulation, we find that the typical covering fraction of the dense, cold gas in 1012 M⊙ haloes at z∼ 2.5 is lower than expected (∼5 per cent). In addition, the absorption signal by the interstellar medium of the galaxy itself turns out to be so deep and so broad in velocity space that it completely drowns that of the filamentary gas. A detectable signal might be obtained from a cold filament exactly aligned with the line of sight, but this configuration is so unlikely that it would require surveying an overwhelmingly large number of candidate galaxies to tease it out. Finally, the predicted metallicity of the cold gas in filaments is extremely low (≤10−3 Z⊙). If this result persists when higher resolution runs are performed, it would significantly increase the difficulty of detecting filamentary gas inflows using metal lines. However, even if we assume that filaments are enriched to Z⊙, the absorption signal that we compute is still weak. We are therefore led to conclude that it is extremely difficult to observationally prove or disprove the presence of cold filaments as the favourite accretion mode of galaxies using low-ionization metal absorption lines. The Lyα emission route looks more promising but due to the resonant nature of the line, radiative transfer simulations are required to fully characterize the observed signal.
MICROBIOLOGICAL QUALITY OF THE GHANAIAN VOLTA CLAM (GALATEA PARADOXA)
Authors: AMOAH CECILIA , BROWN CHARLES , OFORI‐DANSON P. K. , ODAMTTEN GEORGE T. 
Published: March 2011

ABSTRACTMicrobiological quality of the Ghanaian Volta clam (Galatea paradoxa) was assessed by fecal coliform and total aerobic bacteria counts. Polymerase chain reaction (PCR) with universal eubacterial 16S and 23S rDNA primers was also used to show the presence of bacteria in samples of the gut fluid and mantle tissues. The clams were found to be laden with potential pathogenic bacteria identified by conventional methods. Twenty bacteria species were isolated. They were Pseudomonas aeruginosa, Klebsiella ornithinolytica, Flavobacterium meningosepticum, Enterobacter aerogenes, E. agglomerans, E. cloacae, Aeromonas sobria, Acinetobacter sp, Vibrio cholerae, Micrococcus radiodurans, Streptococcus faecalis, Staphylococcus aureus, Escherichia coli, Citrobacter freundii, Chromobacterium violaceum, Morganella morganii, Proteus mirabilis, Salmonella sp., Serratia marcescens and Yersinia intermedia. With PCR, bacterial DNA fragments were detected in 16.7% (2/12) of clam gut and 83.3% (10/12) of mantle tissues. Bacteria load in the clam mantle tissue was higher than in the gut.PRACTICAL APPLICATIONSThe Volta clam is of high commercial interest in the Volta estuary. Information on the presence of potential pathogenic bacteria in this shellfish is thus very important to the food industry, especially in the shellfish‐eating community, where the shellfish is eaten in the semi‐processed ready‐to‐eat state. Data from this article will assist to ensure food safety.
AQUEOUS EXTRACTS FROM THE BULBS OF TULBAGHIA VIOLACEA ARE ANTIFUNGAL AGAINST ASPERGILLUS FLAVUS
Authors: BELEWA V. , BAIJNATH H. , SOMAI B.M. 
Published: March 2011

ABSTRACT Aspergillus flavus is a cosmopolitan fungal pathogen that is often implicated in food spoilage and in aspergillosis infections in immunocompromised individuals. The current study was undertaken to assess the effect of aqueous bulb extracts of Tulbaghia violacea on A. flavus spore survival. The broth dilution assay was used to determine that the minimum inhibitory concentration (MIC) of the extract against A. flavus was 15 mg/mL and that the minimum fungicidal concentration (MFC) was 20 mg/mL. During well diffusion assays A. flavus was inhibited by a minimal extract concentration of 60 mg/mL. As expected, inhibition zones during well diffusion assays increased with increasing extract concentrations. The 2,3‐bis(2‐methoxy‐4‐nitro‐5‐sulfophenyl)‐5‐([phenylamino] carbonyl)‐2H‐tetrazolium hydroxide (XTT) assay showed a direct proportional decrease in metabolic activity of A. flavus with an increase in extract concentration. Inhibition of metabolic activity was quantifiable as early as 12 h after spore inoculation. This was in contrast to the broth dilution assay where inhibition only became clearly observable 48 h after spore inoculation. The antifungal nature of T. violacea aqueous bulb extracts against A. flavus 2527 shown here for the first time indicates a potential use of this extract as an effective antifungal agent against Aspergillus species. The extract could possibly be used in the future for reducing Aspergillus contamination of plant‐derived foods.PRACTICAL APPLICATIONS Tulbaghia violacea (wild garlic) is a well‐known, indigenous ornamental plant in South Africa whose biological activities including a reduction in blood pressure has been previously documented. It is often eaten by African people for alleviating symptoms of high blood pressure including headaches. The plant is therefore safe to consume and appears to have no toxicological effects. With an ever‐increasing population and increasing cost of maintaining a constant and effective food supply, the natural antifungal agents found in aqueous extracts of T. violacea may provide an inexpensive means of reducing food spoilage by Aspergillus spp.
EVALUATION OF THE PERFORMANCE OF BACILLUS CEREUS FOR ASSAY OF TETRACYCLINES IN CHICKEN MEAT
Authors: MWANGI WILLIAM WACHIRA , SHITANDI ANAKALO , NGURE RAPHAEL 
Published: March 2011

ABSTRACTIn the Kenyan poultry industry, there is lack of affordable and easy‐to‐perform antibiotic residue screening methods. The aim of this study was to determine the limits of detection (LODs) of oxytetracycline (OTC) and evaluate whether it is reliably detectable at maximum residue limits (MRLs) in poultry tissues. Microbiological detection was achieved by agar well diffusion using Bacillus cereus, three medium pH and two poultry organs. Inhibition zones increased significantly (P < 0.001) when OTC was detected at a decreasing pH in both the liver and the kidney. OTC was detected below the MRLs of 600 ng/g in the liver and 1,200 ng/g in the kidney. However, growth media pH and antibiotic concentration affected the LODs. B. cereus plate at pH 7 can be used effectively for routine screening for OTC residues. The LODs were 131.3 ng/mL and 33.4 ng/mL, in the liver and the kidney, respectively.PRACTICAL APPLICATIONSApproximately 14.6 tons of active antibiotics are annually used in food animal production in Kenya. Most of the methods for screening of veterinary drugs in animal products are expensive. This is one of the factors that has contributed to the failure of observance of regulations regarding antibiotic residues in most developing countries. The described method can screen for residues of drugs in chicken meat inexpensively, quickly and with ease. This forms a basis for wider adaptability in the developing world so that unsafe samples can be selected from a majority of samples that do not contain drug residues for further quantitative testing.
AUTOMATED BACTERIAL DNA ISOLATION FROM FOOD AND FEED SAMPLES – A BIOPREPAREDNESS DESIGN
Authors: EHRS S. , ÅGREN P. , STEPHANSSON O. , GARBOM S. , ZUMPE A. LUNDIN 
Published: March 2011

ABSTRACTA high‐throughput biosafety level 3 suitable method is necessary for rapid discovery of unknown pathogens in complex matrices from the food chain. In this paper, three automated bacterial DNA extraction methods for real‐time polymerase chain reaction (PCR) detection were evaluated. The systems compared were the Magnatrix (NorDiag, Oslo, Norway) and the EZ1 (Qiagen, Hilden, Germany), both based on purification with magnetic beads, and the ABI Prism™ 6100 Nucleic Acid PrepStation (Applied Biosystems, Foster City, CA), which is based on silica gel filtration. In the comparison, the detection of bacterial DNA from food and feed seeded with one Gram‐positive and one Gram‐negative bacterial species was compared. A wider range of matrices were successfully processed using the magnetic bead‐based DNA extraction robots compared with the silica gel filtration‐based robot. The limit of detection was 102–103 cfu/g matrix of both bacteria in powdered milk, minced meat, whole wheat and soypass using the EZ1 extraction robot.PRACTICAL APPLICATIONSA rapid, wide‐range method to discover unknown dangerous pathogens in complex matrices from the food chain is crucial to minimize the effects in the society of extraordinary events. These events are defined as accidents or unexpected disease outbreaks and deliberate spread of highly pathogenic bacteria. Gaining knowledge of the advantages and disadvantages of extraction methods from complex matrices is important in the development of fast and reliable detection methods.
OCCURRENCE, VIRULENCE GENES AND ANTIMICROBIAL RESISTANCE OF ESCHERICHIA COLI O157 IN BOVINE, CAPRINE, OVINE AND PORCINE CARCASSES IN GREECE
Authors: GOVARIS ALEXANDROS , ANGELIDIS APOSTOLOS S. , KATSOULIS KONSTANTINOS , POURNARAS SPYROS 
Published: March 2011

ABSTRACTOne thousand and two hundred carcasses (620 bovine, 130 caprine, 230 ovine and 220 porcine) from several slaughterhouses throughout Greece were examined for the presence of Escherichia coli O157 after evisceration and before chilling. Twelve E. coli O157 strains (1.0%) were isolated of which eight were from bovine (1.3%), one from caprine (0.8%) and three from ovine (1.3%) meat. None was isolated from pork meat. Six out of the 12 E. coli O157 isolates (50.0%) could be classified as Shiga‐toxigenic based on polymerase chain reaction (PCR), belonged to the E. coli O157:H7 serotype and were PCR‐positive for the stx1, stx2 and eae genes except one isolate that was stx1‐negative. The remaining six E. coli O157 isolates tested negative for Shiga‐toxin production, both by immunoassay and by PCR, were PCR‐negative for the eae gene, and all except one (strain LFH34, a bovine carcass isolate) were also PCR‐negative for the H7 flagellar gene. Among the 15 antimicrobials tested, the E. coli O157 isolates were the most frequently resistant to ticarcillin (9 out of the 12 isolates, 75%).PRACTICAL APPLICATIONSThe Shiga toxin‐producing Escherichia coli (STEC) strains are severe human pathogens. Although the prevalence of STEC in bovine meat has been investigated in several countries worldwide, no such study has been conducted previously in Greece. In addition, very few studies in the literature have reported on the prevalence and characterization of STEC isolated from animal carcasses other than bovine. The findings of the present study are also useful for epidemiological purposes (monitoring the evolution and spread of STEC worldwide).