bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thermal analysis reveals differential effects of various crosslinkers on bovine annulus fibrosis
Authors: Zhu Keng , Slusarewicz Paul , Hedman Tom 
Published: June 2010

AbstractTreatment of a pathological spinal disc in vivo by injection of protein crosslinking reagents to restore the disc's mechanical properties is a new approach to the treatment of degenerative disc disease. In this study, the thermal stability of the collagen in disc annulus was measured by differential scanning calorimetry following treatment with six different crosslinking agents. The crosslinkers used were; L‐threose (LT), genipin (GP), methylglyoxal (MG), 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride (EDC), glutaraldehyde (GA), and proanthrocyanidin (PA). Untreated tissue displayed a prominent peak at about 66–68°C. Comparison of endothermal patterns of untreated and crosslinker‐treated disc annulus tissue samples showed that a new peak appeared at a higher temperature following treatment. The temperature of the new peak qualitatively depended on the crosslinker in the following order GA > MG > GP > PA = EDC > LT, suggesting that the enhanced thermal stability of collagen in the annulus tissue was related to the nature of the crosslinker. Also, the enthalpic ratios of the lower temperature (noncrosslinked) peaks in the treated and untreated tissue, and of the higher and lower temperature peaks in the treated tissue, both indicated that the various agents crosslinked the tissue with different efficiencies. Our data suggest that the ability of GP to penetrate into the disc and form long‐ and short‐range crosslinks may make it the most suitable candidate for clinical development. In addition, binary combinations of long‐ and short‐range crosslinkers, such as PA with LT, may also provide synergistic effects due to their substantially different physicochemical properties. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:8–13, 2011
Adjuvant radiation therapy is associated with improved survival for adenoid cystic carcinoma of the breast
Authors: Coates Jodi M. , Martinez Steve R. , Bold Richard J. , Chen Steven L. 
Published: June 2010

AbstractBackgroundThe role of adjuvant radiation therapy (RT) for adenoid cystic carcinoma (ACC) of the breast remains unclear.Materials and MethodsWe queried the Surveillance, Epidemiology, and End Results database for patients with breast ACC resected between 1988 and 2005, and divided patients based on the receipt of RT. Univariate and multivariate survival comparisons were made for overall and disease‐specific survival.ResultsThree hundred seventy six patients met criteria for inclusion. Demographics and staging were similar between groups. Univariate analysis revealed an absolute overall and cause‐specific survival benefit of 21% and 7% at 10 years (P = 0.005 andP = 0.12 respectively). In the multivariate analysis, RT was a significant predictor of overall and cause‐specific survival with hazard ratios of 0.44 (95% Confidence interval (CI) = 0.22–0.88) and 0.1 (95% CI: 0.01–0.88), respectively.ConclusionsRT after local surgical therapy for ACC of the breast improved both cause‐specific and overall survival. Use of RT in this rare tumor should be considered in patients otherwise eligible for RT. J. Surg. Oncol. 2010;102:342–347. © 2010 Wiley‐Liss, Inc.
Delphian node metastasis in head and neck cancers—Oracle or myth?
Authors: Iyer N. Gopalakrishna , Shaha Ashok R. , Ferlito Alfio , Thomas Robbins K. , Medina Jesus E. , Silver Carl E. , Rinaldo Alessandra , Takes Robert P. , Suárez Carlos , Rodrigo Juan P. , Bradley Patrick J. , Werner Jochen A. 
Published: June 2010

AbstractDelphian node (DN) refers to the pre‐laryngeal or pre‐cricoid nodal tissue often identified during laryngeal or thyroid surgery. The original nomenclature is based on the assumption that metastasis to this node was predictive of aggressive disease and poor outcome for patients. In this article, we review the existing literature on the topic to determine the significance of DN metastasis in laryngeal, hypopharyngeal and thyroid cancers. J. Surg. Oncol. 2010;102:354–358. © 2010 Wiley‐Liss, Inc.
High pretherapeutic thymidylate synthetase and MRP‐1 protein levels are associated with nonresponse to neoadjuvant chemotherapy in oesophageal adenocarcinoma patients
Authors: Langer Rupert , Ott Katja , Feith Marcus , Lordick Florian , Specht Katja , Becker Karen , Hofler Heinz 
Published: June 2010

AbstractBackgroundThe aim of this study was to determine whether pretherapeutic protein expression levels of the excision repair cross‐complementing (ERCC1) enzyme, thymidylate synthetase (TS), multidrug‐resistance protein 1 (MRP‐1) and P‐glycoprotein (P‐gp) are associated with tumour response to cisplatin and fluorouracil (5‐FU)‐based neoadjuvant chemotherapy in oesophageal adenocarcinomasMethodsThe expression levels of ERCC1, TS, MDR‐1 and P‐gp were determined immunohistochemically in pretherapeutic tumour biopsies from 40 oesophageal adenocarcinoma patients and were correlated with histopathological tumour regression and with patient survival. Protein expression was compared to mRNA data, which was previously published for ERCC1, TS and MRP‐1 and newly determined for the purpose of this study for MDR‐1/P‐gp.ResultsHigh‐TS and ‐MRP‐1 protein expression was correlated with tumour non‐response to chemotherapy (P = 0.001 and P = 0.036, respectively). For ERCC‐1 and P‐gp, no association between pretherapeutic protein expression and response was found. There was no correlation between mRNA levels and protein expression for all investigated markers. Survival analysis revealed a trend towards increased survival for low‐ERCC‐1 expression (P = 0.079).ConclusionsThe pattern of pretherapeutic expression of TS and MRP‐1 is related to chemotherapy response in oesophageal adenocarcinoma patients. Immunohistochemical assessment of these markers may be helpful for response prediction. J. Surg. Oncol. 2010;102:503–508. © 2010 Wiley‐Liss, Inc.
pT0 prostate cancer after radical prostatectomy
Authors: Gross Jenna L. , Masterson Timothy A. , Cheng Liang , Johnstone Peter A.S. 
Published: June 2010

AbstractThe purpose of our study was to perform a literature review of current data to determine the frequency and correlates of pT0 prostate cancer after radical prostatectomy alone. A comprehensive search was made of MEDLINE and PUBMED. Seven studies were identified involving 18,135 patients with 74 reported pT0 cases. The most frequent correlates from our pooled data of patients with pT0 specimens include preoperative PSA <10 ng/ml, only one positive core biopsy, and Gleason score <7. J. Surg. Oncol. 2010;102:331–333. © 2010 Wiley‐Liss, Inc.
Comparison between a linear ion trap and a triple quadruple MS in the sensitive detection of large peptides at femtomole amounts on column
Authors: Vatansever Bilgin , Lahrichi Sabine Laila , Thiocone Aly , Salluce Nunzio , Mathieu Marc , Grouzmann Eric , Rochat Bertrand 
Published: June 2010

AbstractIn addition to the importance of sample preparation and extract separation, MS detection is a key factor in the sensitive quantification of large undigested peptides. In this article, a linear ion trap MS (LIT‐MS) and a triple quadrupole MS (TQ‐MS) have been compared in the detection of large peptides at subnanomolar concentrations. Natural brain natriuretic peptide, C‐peptide, substance P and D‐Junk‐inhibitor peptide, a full D‐amino acid therapeutic peptide, were chosen. They were detected by ESI and simultaneous MS1 and MS2 acquisitions. With direct peptide infusion, MS2 spectra revealed that fragmentation was peptide dependent, milder on the LIT‐MS and required high collision energies on the TQ‐MS to obtain high‐intensity product ions. Peptide adsorption on surfaces was overcome and peptide dilutions ranging from 0.1 to 25 nM were injected onto an ultra high‐pressure LC system with a 1 mm id analytical column and coupled with the MS instruments. No difference was observed between the two instruments when recording in LC‐MS1 acquisitions. However, in LC‐MS2 acquisitions, a better sensitivity in the detection of large peptides was observed with the LIT‐MS. Indeed, with the three longer peptides, the typical fragmentation in the TQ‐MS resulted in a dramatic loss of sensitivity (≥10×).
Characterization of bacterial lipid profiles by using rapid sample preparation and fast comprehensive two‐dimensional gas chromatography in combination with mass spectrometry
Authors: Purcaro Giorgia , Tranchida Peter Quinto , Dugo Paola , Camera Erminia La , Bisignano Giuseppe , Conte Lanfranco , Mondello Luigi 
Published: June 2010

AbstractThe bacteria fatty acid profile has been extensively studied for taxonomic classification purposes, since bacteria, in general, contain particular and rare fatty acids, compared with animal and plant tissues. As for any real‐world sample type, the development of rapid and reliable methods for (i) sample identification (in this case, bacterium type), and (ii) constituent identification (in this instance, the fatty acid profile) is desirable. In this research, a half‐an‐hour procedure, to analyze bacteria, was developed: a 2‐min one‐step sample preparation step was followed by a relatively fast comprehensive 2D GC‐MS separation (25 min). Furthermore, dedicated MS libraries were constructed for the identification of bacteria and fatty acids. Finally, data processing, only qualitative at this stage, was carried out with the support of a novel comprehensive 2D GC software.
Kinetic study of the gas‐phase reaction of hydroxyl radical with CF3CH2OCH2CF3 using the laser photolysis–laser induced fluorescence method
Authors: Sengupta S. , Indulkar Y. , Kumar A. , Dhanya S. , Naik P. D. , Bajaj P. N. 
Published: June 2010

AbstractThe laser photolysis‐laser‐induced fluorescence method was used for measuring the kinetic parameters of the reaction of OH radicals with CF3CH2OCH2CF3 (2,2,2‐trifluoroethyl ether), in the temperature range of 298–365 K. The bimolecular rate coefficient at 298 K, kII(298), was measured to be (1.47 ± 0.03) × 10−13 cm3 molecule−1 s−1, and the temperature dependence of kII was determined to be (4.5 ± 0.8) × 10−12exp [−(1030 ± 60)/T] cm3 molecule−1 s−1. The error quoted is 1σ of the linear regression of the respective plots. The rate coefficient at room temperature is very close to the average of the three previous measurements, whereas the values of Ea/R and the A‐factor are higher than the two previously reported values. © 2010 Wiley Periodicals, Inc. Int J Chem Kinet 42: 519–525, 2010
Graded aluminium material by incremental bulk forming
Authors: Neugebauer R. , Lampke T. , Dietrich D. , Bergmann M. 
Published: June 2010

AbstractEin stranggepresstes massives Halbzeug der Aluminiumlegierung EN AW‐6082 wurde durch ein inkrementelles Walzverfahren umgeformt. Das als Gradierungswalzen bezeichnete Verfahren ist durch stark inhomogene Verformungen gekennzeichnet. Lokal extreme Dehnungen führen zu einer Hochverformung in definierten Bereichen des Halbzeuges mit der Ausbildung eines Gradienten der Korngröße. Am erzeugten Gradientenwerkstoff wurde mittels registrierender Eindringhärteprüfung die Vickershärte bestimmt. Die Gefügecharakterisierung erfolgte mittels Kristallitorientierungskartographie im Rasterelektronenmikroskop (EBSD). Der Vergleich des Ausgangszustandes mit dem verformten Halbzeug zeigt in Abhängigkeit vom Grad der Verformung einen zunehmenden Anteil kleiner Körner, der von einer Texturänderung begleitet ist. Anhand der Korngrößenverteilungen wird belegt, dass die Kornfeinung des ursprünglich grobkristallinen Werkstoffs bis hin zu Korngrößen unter 1 μm erfolgt. Eine starke Härtesteigerung im Randbereich ist weiteres Indiz für den Kornfeinungseffekt.An extruded bulk semi‐finished product of the aluminium alloy EN AW‐6082 was processed by an incremental rolling process. The applied method, termed gradiation rolling, is characterized by highly inhomogeneous deformation. Local extreme strain generation leads to a severe plastic deformation in defined areas of the billet volume resulting in the formation of a gradient of the grain size. Characteristics of the processed material were determined. The Vickers microhardness was verified. A microstructural characterization by crystal orientation mapping (EBSD analysis) was carried out. The comparison of the deformed material with the initial state shows an increasing proportion of small grains related to the degree of deformation. A corresponding change of the texture can be observed. The grain refinement of the original coarse crystalline material to grain sizes below 1 μm is demonstrated. A large increase of the hardness in the near suface area is further indication of the grain refining effect.
Formation of columnar microstructure when brazing steels as well as nickel and titanium alloys
Authors: Bach Fr.‐W. , Holländer U. , Möhwald K. , Nicolaus M. 
Published: June 2010

AbstractSeit den 50er Jahren des 20. Jahrhunderts ist bekannt, dass sich beim Löten mit reinem Kupfer von Stählen unterschiedlichen Kohlenstoffgehalts Stängelkristallite in der Lötnaht bilden. Gelötete Verbindungen mit einem solchen Gefügeaufbau zeichnen sich durch eine grundwerkstoffähnliche Festigkeit aus, wenn die Stängelkristallite die Lötnaht durchdringen. Mit Hilfe thermodynamischer Beziehungen und grundlegenden Transportgleichungen wurde ein physikalisches Modell entwickelt, mit dem die Stängelkristallitbildung in Abhängigkeit der relevanten Parameter quantitativ beschrieben werden kann. Das hierzu für das Lotsystem Fe∣C∣Cu experimentell verifizierte Modell [1] lässt sich unter Berücksichtigung der notwendigen Voraussetzungen auf andere Werkstoffsysteme anwenden. Weiterhin wurde das Modell auf hochlegierte Stähle sowie auf Nickel‐ und Titanlegierungen erweitert.Since the 1950s it is known that a formation of columnar microstructure occurs in the seam when brazing steels with different carbon content to liquid copper. Brazed joints with such a microstructure are characterised by a base‐material‐like strength if the crystallites penetrate the seam. With the aid of thermodynamic relations and fundamental transport properties a physical model was developed which describes the columnar microstructure formation as a function of relevant parameters. The model can be applied in principle to any brazing system with metallurgical properties comparable to those of the Fe‐C‐Cu system. Furthermore the model was expand to high‐alloyed steels as well as nickel and titanium alloys.
X‐ray diffraction residual stress analysis on PVD‐multilayer coatings
Authors: Tillmann W. , Selvadurai‐Laßl U. , Vogli E. , Fischer G. , Hoffmann F. 
Published: June 2010

AbstractFür die verarbeitende Industrie werden zunehmend harte und verschleißfeste Beschichtungen mit hoher Lebensdauer entwickelt. Keramische PVD‐Schichten besitzen bereits eine hohe Härte und Verschleißfestigkeit. Allerdings weisen derartige Schichten eine begrenzte Lebensdauer durch eine niedrige Duktilität auf. Zur Erhöhung ihrer Lebensdauer beim industriellen Einsatz werden zunehmend Multilayer weiterentwickelt. Dieser Beitrag stellt die ersten Untersuchungsergebnisse zum Einfluss der Substratvorbehandlung, des Schichtdesigns und der Prozessparameter von mehrlagigen metall‐keramischen PVD‐Schichten auf die Eigenschaften eines innovativen Gesamtverbundes, Ti/TiAlN und Cr/CrAlN auf Stahl vor. Sie belegen, dass die Vorbehandlung des Substrats und das Schichtdesign einen deutlichen Einfluss auf die Schichtmerkmale und den Eigenspannungszustand ausüben.Hard and wear resistant thin layers provides significant improvements in tools employed in manufacturing industry and are recently of great interest to increase as well as to enhance the tools’ performance and lifetime. Ceramic PVD‐layers already feature a high hardness combined with a high abrasive wear resistance. However, such layers possess only a limited lifetime due to their low toughness. To increase the toughness as well as the durability of such layers multilayer systems are steadily developed. This research work presents the first results of the influence of the steel substrate pretreatments, multilayer designs and coating process parameters on the properties of two innovative PVD metal‐ceramic multilayers, Ti/TiAlN and Cr/CrAlN. It was obviously that the substrate pretreatment and the multilayer design have a large influence on the layer properties and residual stresses.
Sensory alien hand syndrome in corticobasal degeneration: A cerebral blood flow study
Authors: Delrieu Julien , Payoux Pierre , Toulza Olivier , Esquerre Jean‐Paul , Vellas Bruno , Voisin Thierry 
Published: June 2010

AbstractThe presence of alien hand syndrome (AHS) is suggestive of the diagnosis of corticobasal degeneration when it develops in a progressive way. Sensory AHS (sAHS) should be distinguished from the motor form described more commonly. The physiopathology of sAHS remains unclear. The aim of this study is to determine cerebral regions involved in sAHS. We compared perfusion single photon emission computer tomography scans of patients with sAHS (n = 3) and without (n = 4). We observed significant decrease of regional cerebral blood flow over the nondominant thalamus in sAHS+ compared to sAHS− patients. This result suggests the involvement of the nondominant thalamus in sAHS. © 2010 Movement Disorder Society
Definition and classification of hyperkinetic movements in childhood
Authors: Sanger Terence D. , Chen Daofen , Fehlings Darcy L. , Hallett Mark , Lang Anthony E. , Mink Jonathan W. , Singer Harvey S. , Alter Katharine , Ben‐Pazi Hilla , Butler Erin E. , Chen Robert , Collins Abigail , Dayanidhi Sudarshan , Forssberg Hans , Fowler Eileen , Gilbert Donald L. , Gorman Sharon L. , Gormley Mark E. , Jinnah H. A. , Kornblau Barbara , Krosschell Kristin J. , Lehman Rebecca K. , MacKinnon Colum , Malanga C. J. , Mesterman Ronit , Michaels Margaret Barry , Pearson Toni S. , Rose Jessica , Russman Barry S. , Sternad Dagmar , Swoboda Kathy J. , Valero‐Cuevas Francisco 
Published: June 2010

AbstractHyperkinetic movements are unwanted or excess movements that are frequently seen in children with neurologic disorders. They are an important clinical finding with significant implications for diagnosis and treatment. However, the lack of agreement on standard terminology and definitions interferes with clinical treatment and research. We describe definitions of dystonia, chorea, athetosis, myoclonus, tremor, tics, and stereotypies that arose from a consensus meeting in June 2008 of specialists from different clinical and basic science fields. Dystonia is a movement disorder in which involuntary sustained or intermittent muscle contractions cause twisting and repetitive movements, abnormal postures, or both. Chorea is an ongoing random‐appearing sequence of one or more discrete involuntary movements or movement fragments. Athetosis is a slow, continuous, involuntary writhing movement that prevents maintenance of a stable posture. Myoclonus is a sequence of repeated, often nonrhythmic, brief shock‐like jerks due to sudden involuntary contraction or relaxation of one or more muscles. Tremor is a rhythmic back‐and‐forth or oscillating involuntary movement about a joint axis. Tics are repeated, individually recognizable, intermittent movements or movement fragments that are almost always briefly suppressible and are usually associated with awareness of an urge to perform the movement. Stereotypies are repetitive, simple movements that can be voluntarily suppressed. We provide recommended techniques for clinical examination and suggestions for differentiating between the different types of hyperkinetic movements, noting that there may be overlap between conditions. These definitions and the diagnostic recommendations are intended to be reliable and useful for clinical practice, communication between clinicians and researchers, and for the design of quantitative tests that will guide and assess the outcome of future clinical trials. © 2010 Movement Disorder Society
Traveling wave solutions for a generalized Ostrovsky equation
Authors: Gandarias M. L. , Bruzon M. S. 
Published: June 2010

AbstractIn this paper looking for traveling wave solutions, we find that when the polynomial of velocity is quintic the generalized Ostrovsky equation (GOE) has abundant exact solutions that can be expressed in terms of the Jacobi elliptic functions. Hence, the GOE has a plenty of periodic waves, solitary waves, compactons, etc. These solutions are derived from the solutions of a simple non‐linear ordinary differential equation. Copyright © 2010 John Wiley & Sons, Ltd.
Instant coffee with high chlorogenic acid levels protects humans against oxidative damage of macromolecules
Authors: Hoelzl Christine , Knasmüller Siegfried , Wagner Karl‐Heinz , Elbling Leonilla , Huber Wolfgang , Kager Nina , Ferk Franziska , Ehrlich Veronika , Nersesyan Armen , Neubauer Oliver , Desmarchelier Aurélien , Marin‐Kuan Maricel , Delatour Thierry , Verguet Clotilde , Bezençon Claudine , Besson Amélie , Grathwohl Dominik , Simic Tatjana , Kundi Michael , Schilter Benoît , Cavin Christophe 
Published: June 2010

AbstractScope: Coffee is among the most frequently consumed beverages. Its consumption is inversely associated to the incidence of diseases related to reactive oxygen species; the phenomenon may be due to its antioxidant properties. Our primary objective was to investigate the impact of consumption of a coffee containing high levels of chlorogenic acids on the oxidation of proteins, DNA and membrane lipids; additionally, other redox biomarkers were monitored in an intervention trial.Methods and results: The treatment group (n=36) consumed instant coffee co‐extracted from green and roasted beans, whereas the control consumed water (800 mL/P/day, 5 days). A global statistical analysis of four main biomarkers selected as primary outcomes showed that the overall changes are significant. 8‐Isoprostaglandin F2α in urine declined by 15.3%, 3‐nitrotyrosine was decreased by 16.1%, DNA migration due to oxidized purines and pyrimidines was (not significantly) reduced in lymphocytes by 12.5 and 14.1%. Other markers such as the total antioxidant capacity were moderately increased; e.g. LDL and malondialdehyde were shifted towards a non‐significant reduction.Conclusion: The oxidation of DNA, lipids and proteins associated with the incidence of various diseases and the protection against their oxidative damage may be indicative for beneficial health effects of coffee.
Differentiation between [1,2,4]triazolo[1,5‐a] pyrimidine and [1,2,4]triazolo[4,3‐a]‐ pyrimidine regioisomers by 1H15N HMBC experiments
Authors: Salgado Antonio , Varela Carmen , García Collazo Ana María , Pevarello Paolo 
Published: June 2010

AbstractThe condensation of malonoaldehyde derivatives with either a 3‐amino‐[1,2,4]‐triazole or a 3,5‐diamino‐[1,2,4]‐triazole precursor was studied. In agreement with previous reports, two different bicycles, namely, bearing the regioisomeric [1,2,4]triazolo[1,5‐a]pyrimidine (1) or[1,2,4] triazolo [4,3‐a]pyrimidine (2) structural surrogates, could be obtained. We found that, depending on the triazole precursor, only one regioisomer resulted, either of the 1 or 2 series. We also observed that these two structural surrogates could be unambiguously differentiated by indirectly measuring their 15N chemical shifts by 1H15N HMBC experiments. The occasional conversion of [1,2,4]triazolo[4,3‐a]pyrimidines to the [1,2,4]triazolo[1,5‐a]pyrimidine counterparts could be unequivocally determined by 15N NMR data. Copyright © 2010 John Wiley & Sons, Ltd.