bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association of serum uric acid with hypertriglyceridemia in children and adolescents: a cross-sectional study
Authors: Si Shang-An , Chen Meng-Qi , Zhang Gui-Ju 
Published: June 2024

Abstract
Background
Uric acid (UA), a liver-derived metabolite, is intimately tied to metabolic disorders. Although ample research underscores its connection with hypertriglyceridemia (HTG), studies focusing on adolescents remain limited. To fill the gaps in epidemiology,this study focused on analyzing the relationship between the levels of uric acid and HTG in a demographic sample comprising adolescents from the United States.

Methods
In this study, a total of 4,435 participants through the National Health and Nutrition Examination Survey (NHANES) from 2011 to 2020. The exposure variable was serum uric acid (SUA), the effect variable was HTG, and the covariates included demographic, questionnaire, physical examination and laboratory indicators. We utilized weighted logistic regression and meticulous subgroup evaluations to discern the intrinsic link between SUA and HTG. Stratified analyses augmented the validation of this association, while smooth curve fitting probed for potential nonlinear correlations.

Results
The study included 4,435 participants. Male adolescents exhibit elevated SUA levels. After adjusting for all variables, the weighted multiple logistic regression model revealed that SUA was positively correlated with HTG risk (OR = 1.006, 95% CI: 1.005–1.007). This relationship was consistent across the three tertiles group of SUA (T1: OR = 1.006 [95% CI: 1.005–1.007]; T2: OR = 1.006 [95% CI: 1.005–1.007]; T3: OR = 1.004 [95% CI: 1.003–1.006]; P for trend < 0.001). Stratified analyses confirmed that the positive correlation between SUA and HTG risk was significant, irrespective of sex, age or race.

Conclusions
In American children and adolescents aged 12 to 18 years, there was a pronounced association between SUA and HTG. SUA could serve as a risk indicator for HTG. It is recommended that children diagnosed with HTG should be regularly tested for SUA levels. In addition, it is recommended that SUA be included in the comprehensive care of children diagnosed with HTG.
A systematic review of experimentally tested implementation strategies across health and human service settings: evidence from 2010-2022
Authors: Ashcraft Laura Ellen , Goodrich David E. , Hero Joachim , Phares Angela , Bachrach Rachel L. , Quinn Deirdre A. , Qureshi Nabeel , Ernecoff Natalie C. , Lederer Lisa G. , Scheunemann Leslie Page , Rogal Shari S. , Chinman Matthew J. 
Published: June 2024

Abstract
Background
Studies of implementation strategies range in rigor, design, and evaluated outcomes, presenting interpretation challenges for practitioners and researchers. This systematic review aimed to describe the body of research evidence testing implementation strategies across diverse settings and domains, using the Expert Recommendations for Implementing Change (ERIC) taxonomy to classify strategies and the Reach Effectiveness Adoption Implementation and Maintenance (RE-AIM) framework to classify outcomes.

Methods
We conducted a systematic review of studies examining implementation strategies from 2010-2022 and registered with PROSPERO (CRD42021235592). We searched databases using terms “implementation strategy”, “intervention”, “bundle”, “support”, and their variants. We also solicited study recommendations from implementation science experts and mined existing systematic reviews. We included studies that quantitatively assessed the impact of at least one implementation strategy to improve health or health care using an outcome that could be mapped to the five evaluation dimensions of RE-AIM. Only studies meeting prespecified methodologic standards were included. We described the characteristics of studies and frequency of implementation strategy use across study arms. We also examined common strategy pairings and cooccurrence with significant outcomes.

Findings
Our search resulted in 16,605 studies; 129 met inclusion criteria. Studies tested an average of 6.73 strategies (0-20 range). The most assessed outcomes were Effectiveness (n=82; 64%) and Implementation (n=73; 56%). The implementation strategies most frequently occurring in the experimental arm were Distribute Educational Materials (n=99), Conduct Educational Meetings (n=96), Audit and Provide Feedback (n=76), and External Facilitation (n=59). These strategies were often used in combination. Nineteen implementation strategies were frequently tested and associated with significantly improved outcomes. However, many strategies were not tested sufficiently to draw conclusions.

Conclusion
This review of 129 methodologically rigorous studies built upon prior implementation science data syntheses to identify implementation strategies that had been experimentally tested and summarized their impact on outcomes across diverse outcomes and clinical settings. We present recommendations for improving future similar efforts.
A novel SALL1 C757T mutation in a Chinese family causes a rare disease --Townes-Brocks syndrome
Authors: Chi Yunqian , Yao Yi , Sun Futao , Zhang Wenhong , Zhang Zihan , Wang Yunhe , Hao Wei 
Published: June 2024

Abstract
Background
Townes-Brocks syndrome (TBS) is a rare genetic disorder characterized by imperforate anus, dysplastic ears, thumb malformations, and other abnormalities. Previous studies have revealed that mutations in the SALL1 gene can disrupt normal development, resulting in the characteristic features of Townes-Brocks syndrome. Spalt-like transcription factors (SALLs) are highly conserved proteins that play important roles in various cellular processes, including embryonic development, cell differentiation, and cell survival. Over 400 different variants or mutations have been reported in the SALL1 gene in individuals with TBS. Most of these variants lead to the formation of premature termination codons (PTCs), also known as nonsense mutations. The majority of these PTCs occur in a specific region of the SALL1 gene called the “hotspot region”, which is particularly susceptible to mutation.

Methods
In this study, we conducted whole-exome sequencing on a three-generation Chinese family with anorectal malformations.

Results
We identified a novel heterozygous mutation (chr16:51175376:c.757 C > T p.Gln253*) in the SALL1 gene. Molecular analysis revealed a heterozygous C to T transition at nucleotide position 757 in exon 2 of the SALL1 (NM_002968) gene. This mutation is predicted to result in the substitution of the Gln253 codon with a premature stop codon (p.Gln253*). The glutamine-rich domain forms a long alpha helix, enabling the mutant protein to interact with the wild-type SALL1 protein. This interaction may result in steric hindrance effects on the wild-type SALL1 protein.

Conclusions
Our findings have expanded the mutation database of the SALL1 gene, which is significant for genetic counseling and clinical surveillance in the affected family. Furthermore, our study enhances the understanding of Townes-Brocks syndrome and has the potential to improve its diagnosis and treatment.
Comparative analysis of lipid and flavonoid biosynthesis between Pongamia and soybean seeds: genomic, transcriptional, and metabolic perspectives
Authors: Liu Chun , Huang Rui , Zhao Xingkun , Xu Ranran , Zhang Jianyu , Li Xinyong , Liu Guodao , Dong Rongshu , Liu Pandao 
Published: June 2024

Abstract
Background
Soybean (Glycine max) is a vital oil-producing crop. Augmenting oleic acid (OA) levels in soybean oil enhances its oxidative stability and health benefits, representing a key objective in soybean breeding. Pongamia (Pongamia pinnata), known for its abundant oil, OA, and flavonoid in the seeds, holds promise as a biofuel and medicinal plant. A comparative analysis of the lipid and flavonoid biosynthesis pathways in Pongamia and soybean seeds would facilitate the assessment of the potential value of Pongamia seeds and advance the genetic improvements of seed traits in both species.

Results
The study employed multi-omics analysis to systematically compare differences in metabolite accumulation and associated biosynthetic genes between Pongamia seeds and soybean seeds at the transcriptional, metabolic, and genomic levels. The results revealed that OA is the predominant free fatty acid in Pongamia seeds, being 8.3 times more abundant than in soybean seeds. Lipidomics unveiled a notably higher accumulation of triacylglycerols (TAGs) in Pongamia seeds compared to soybean seeds, with 23 TAG species containing OA. Subsequently, we identified orthologous groups (OGs) involved in lipid biosynthesis across 25 gene families in the genomes of Pongamia and soybean, and compared the expression levels of these OGs in the seeds of the two species. Among the OGs with expression levels in Pongamia seeds more than twice as high as in soybean seeds, we identified one fatty acyl-ACP thioesterase A (FATA) and two stearoyl-ACP desaturases (SADs), responsible for OA biosynthesis, along with two phospholipid:diacylglycerol acyltransferases (PDATs) and three acyl-CoA:diacylglycerol acyltransferases (DGATs), responsible for TAG biosynthesis. Furthermore, we observed a significantly higher content of the flavonoid formononetin in Pongamia seeds compared to soybean seeds, by over 2000-fold. This difference may be attributed to the tandem duplication expansions of 2,7,4ʹ-trihydroxyisoflavanone 4ʹ-O-methyltransferases (HI4ʹOMTs) in the Pongamia genome, which are responsible for the final step of formononetin biosynthesis, combined with their high expression levels in Pongamia seeds.

Conclusions
This study extends beyond observations made in single-species research by offering novel insights into the molecular basis of differences in lipid and flavonoid biosynthetic pathways between Pongamia and soybean, from a cross-species comparative perspective.
Prognostic factors for recurrent instability in recreational athletes following arthroscopic Bankart repair: a retrospective study with an average 4.1-year follow-up
Authors: Zhang Chunsen , Yang Songyun , Wang Jiapeng , Shao Wenze , Huang Yizhou , Tang Xin 
Published: June 2024

Abstract
Background
Extensive research has been conducted to investigate the short-term and long-term outcomes of arthroscopic Bankart repair, yielding varying results across different populations. However, there remains a dearth of studies specifically focused on evaluating outcomes in recreational athletes.

Methods
A retrospective case series study was conducted on recreational athletes who underwent isolated arthroscopic Bankart repair between 2013 and 2021. The primary outcome assessed was recurrent instability, defined as dislocation or subluxation. Secondary outcomes included patient satisfaction, rates of returning to the same sports (RTS) and RTS at preinjury level, and patient-reported outcomes. Evaluation of the Rowe score, Constant score, American Shoulder and Elbow Surgeons score, and VAS pain score were performed. Prognostic factors for recurrent instability, including demographic and clinical characteristics, as well as postoperative magnetic resonance imaging (MRI) appearance of the labrum were analyzed.

Results
A total of 191 patients met the selection criteria, with 150 (78.5%) available for the final follow-up. Recurrent instability occurred in 10.7% of patients, with a mean follow-up duration of 4.1 years. Younger age at surgery and more critical glenoid bone loss were significantly associated with recurrent instability (p = .038 and p = .011, respectively). The satisfaction rate regarding surgery was 90.0%. Rates of return to the same sports (RTS) and RTS at preinjury level were 82.0% and 49.3%, respectively. Clinical outcomes measured at the final follow-up were as follows: Rowe score − 92.8; Constant score − 98.0; ASES score − 98.3; VAS pain score − 0.2. Patients with recurrent instability had significantly inferior outcomes in terms of satisfaction rate, RTS at preinjury level rate, Rowe score, and Constant score (p = .000, p = .039, p = .000, and p = .015, respectively). A total of thirty-seven patients underwent MRI examination six months after surgery in our institution. The T2-weighted anterior labrum morphology was found to be poorer in patients with recurrent instability. No significant difference was observed between patients with or without recurrent instability in terms of anterior Slope, anterior labral glenoid height index (LGHI), inferior Slope, inferior LGHI, and T2-weighted inferior labrum morphology.

Conclusion
Arthroscopic Bankart repair can yield satisfactory medium-term outcomes for recreational athletes. Younger age at surgery, more critical glenoid bone loss, and poorer T2-weighted anterior labrum morphology assessed six months postoperatively were significantly associated with recurrent instability.
Lithium and its effects: does dose matter?
Authors: Manchia Mirko , Paribello Pasquale , Pinna Martina , Steardo Luca , Carpiniello Bernardo , Pinna Federica , Pisanu Claudia , Squassina Alessio , Hajek Tomas 
Published: June 2024

Abstract
Background
Decades of clinical research have demonstrated the efficacy of lithium in treating acute episodes (both manic and depressive), as well as in preventing recurrences of bipolar disorder (BD). Specific to lithium is its antisuicidal effect, which appears to extend beyond its mood-stabilizing properties. Lithium’s clinical effectiveness is, to some extent, counterbalanced by its safety and tolerability profile. Indeed, monitoring of lithium levels is required by its narrow therapeutic index. There is consensus that adequate serum levels should be above 0.6 mEq/L to achieve clinical effectiveness. However, few data support the choice of this threshold, and increasing evidence suggests that lithium might have clinical and molecular effects at much lower concentrations.

Content
This narrative review is aimed at: (1) reviewing and critically interpreting the clinical evidence supporting the use of the 0.6 mEq/L threshold, (2) reporting a narrative synthesis of the evidence supporting the notion that lithium might be effective in much lower doses. Among these are epidemiological studies of lithium in water, evidence on the antisuicidal, anti-aggressive, and neuroprotective effects, including efficacy in preventing cognitive impairment progression, Alzheimer’s disease (AD), and amyotrophic lateral sclerosis (ALS), of lithium; and (3) revieweing biological data supporting clinically viable uses of lithium at low levels with the delineation of a mechanistic hypothesis surrounding its purported mechanism of action. The study selection was based on the authors’ preference, reflecting the varied and extensive expertise on the review subject, further enriched with an extensive pearl-growing strategy for relevant reviews and book sections.

Conclusions
Clinical and molecular effects of lithium are numerous, and its effects also appear to have a certain degree of specificity related to the dose administered. In sum, the clinical effects of lithium are maximal for mood stabilisation at concentrations higher than 0.6 mEq/l. However, lower levels may be sufficient for preventing depressive recurrences in older populations of patients, and microdoses could be effective in decreasing suicide risk, especially in patients with BD. Conversely, lithium’s ability to counteract cognitive decline appears to be exerted at subtherapeutic doses, possibly corresponding to its molecular neuroprotective effects. Indeed, lithium may reduce inflammation and induce neuroprotection even at doses several folds lower than those commonly used in clinical settings. Nevertheless, findings surrounding its purported mechanism of action are missing, and more research is needed to investigate the molecular targets of low-dose lithium adequately.
Exonuclease III assisted exponential amplification reaction (EXPAR) for specific miRNA-155 analysis during post-anesthetic nursing
Authors: Wu Xuejun , Zou Shaolan , Dai Jingshen 
Published: June 2024

AbstractThe persistent obstacle in precise and sensitive identification of microRNAs (miRNAs) pertains to the advancement of expeditious and effective isothermal amplification methodologies suitable for point-of-care environments and monitoring the cancer prognosis in patients receiving post-anesthetic nursing. The exponential amplification reaction (EXPAR) has attracted considerable interest due to its simplicity and ability to rapidly amplify signals. The practical application of the EXPAR is, nevertheless, severely hampered by the inability to differentiate closely related homologous sequences and to modify the designed templates to suit other targets. A loop-stem template for the EXPAR system was developed in this study to facilitate specific target recognition with the aid of exonuclease III (Exo III). This innovation effectively eliminated non-specific hybridization that could occur between the template and interfering sequences, thereby ensuring minimal background amplification of EXPAR. By modulating Exo III-based target recycling, EXPAR based chain amplification and G4/hemin based color reaction, this method facilitated the precise and sensitive examination of miRNA-155, yielding acceptable yields and a minimal detection limit of 0.43 fM. The approach expedites simple and expeditious molecular diagnostic applications involving short nucleic acids and offers an innovative method for enhancing the selectivity of EXPAR-based techniques, providing a robust tool for monitoring the expression level from patients receiving post-anesthetic nursing and guiding the treatment strategy.
Impact and recovery of forest cover following wildfire in the Northern Rocky Mountains of the United States
Authors: Epstein Margaret D. , Seielstad Carl A. , Moran Christopher J. 
Published: June 2024

Abstract
Background
Anthropogenic climate change is expected to catalyze forest conversion to grass and shrublands due to more extreme fire behavior and hotter and drier post-fire conditions. However, field surveys in the Northern Rocky Mountains of the United States show robust conifer regeneration on burned sites. This study utilizes a machine learning (GBM) approach to monitor canopy cover systematically on a census of burned areas in two large wilderness areas from 1985 to 2021, to contextualize these recent field surveys and create a monitoring baseline for future change.

Results
A predictive model was developed from coincident LiDAR and Landsat observations and used to create time series of canopy cover on 352 burned sites (individual wildfires subset by number of times burned), which were then summarized using fire impact and recovery metrics. Fire impact, defined as canopy cover loss relative to pre-fire condition, was highly correlated with burn severity (Spearman’s R = 0.70). Recovery was characterized by the following: (1) whether a burned area began gaining canopy cover and (2) how long would it take to reach pre-fire cover given observed rates of gain. Eighty-five percent of the land area studied showed evidence of recovery. Areas that are failing to recover are burning more recently than their recovering counterparts, with 60% of non-recovering sites burning for the first time after 2003. However, the 5-year probability of recovery is similar among recent burns and for those that burned earlier in the record, suggesting that they may recover with more time. Once sites begin recovering, median time to reach pre-fire cover is 40 years. Seven sites have expected recovery times greater than 200 years, six of which burned for the first time after 2006.

Conclusion
Overall, burned sites in wilderness areas of the Northern Rocky Mountains are broadly recovering from wildfire. However, anthropogenic climate change adds a layer of uncertainty to the future prognosis of conifer recovery. This work provides a framework for systematic monitoring into the future and establishes a baseline of impact and recovery in the mountains of western Montana and northern Idaho.
Association of Age at Menarche With Inflammation and Glucose Metabolism Biomarkers in US Adult Women: NHANES 1999-2018
Authors: Santos Maria P , Bazzano Lydia , Carmichael Owen , O’Bryant Sid , Hsia Daniel S , He Jiang , Ley Sylvia H 
Published: June 2024

Abstract

Context
Early age at menarche (AAM) is a risk factor for type 2 diabetes later in life, but the pathogenic pathways that confer increased risk remain unknown.

Objective
We examined the associations between AAM and inflammatory and glucose metabolism biomarkers among US adult women who were free of diabetes.

Methods
Using the National Health and Nutrition Examination Survey (NHANES) 1999-2018, 19 228 women over 20 years old who were free of self-reported cancer and diabetes were included in this cross-sectional analysis. AAM was the self-reported age at first menstruation. C-reactive protein (CRP), fasting glucose, fasting insulin, and ferritin levels were measured as biomarkers of inflammation and glucose metabolism in adult blood samples using latex-enhanced nephelometry, enzymatic, and immunoassay methods. Multiple linear regression was used to relate AAM to the biomarkers.

Results
The median age at the time of blood sample collection was 44 years (interquartile range, 33-62). After age adjustment, there was an association between a lower AAM and higher CRP (P-trend = .006), fasting glucose (P-trend &lt; .0001), fasting insulin (P-trend &lt; .0001), and ferritin (P-trend &lt; .0001). These remained significant after additional adjustment for demographic, reproductive, lifestyle, and adiposity variables, except for ferritin. Smoking modified the effect of AAM on CRP (P-interaction = .014), fasting insulin (P-interaction &lt; .001), and fasting glucose (P-interaction &lt; .001). In stratified analysis, the observed associations became more pronounced in nonsmokers, while they were attenuated to nonsignificance in active smokers.

Conclusion
Earlier age at menarche is associated with an unfavorable inflammatory and glucose metabolic biomarker profile in a nationally representative sample of adult women free of diabetes, especially among nonsmokers.
Neokantyści Wilhelm Windelband, Heinrich Rickert i Ernst Cassirer wobec filozofii renesansu
Authors: Kubalica Tomasz 
Published: June 2024

Neo-Kantianism as a philosophical formation is characterised primarily by its reference to the philosophy of Immanuel Kant. Nevertheless, to say that the essence of Neo-Kantianism was solely a renewal of Kant's philosophy is insufficient. Also specific to this formation was the adaptation of Kant's thought to the new chalenges of philosophy at the turn of the nineteenth and twentieth centuries. A uniquely exciting analogy for Neo-Kantian philosophy is its reference to the thought of the Renaissance period. Just as the Renaissance marked the revival of many ancient philosophical currents (Platonism, Neoplatonism, Aristotelianism, Stoicism, Epicureanism, Scepticism and even Ionian natural philosophy) in the early modern period, Neo-Kantianism represented a kind of Renaissance of Kant’s philosophy. This affinity of thought is suggested by representatives of the two most important Neo-Kantian schools: Wilhelm Windelband, Heinrich Rickert and Ernst Cassirer. This article focuses on this historical-philosophical parallel by examining the relationship between Neo-Kantianism and Renaissance philosophy. The results show that Neo-Kantianism is not limited to an apologetic repetition of Kant’s philosophy but constitutes a heterodox and critical renewal of it. Such an apologetic approach did not work in modern philosophy, which is dominated by the spirit of criticism. Thus, the Neo-Kantian formation shows deep connections with other periods in the history of philosophy, such as Renaissance philosophy, as a breakthrough period initiating a modern mode of reflection. Understanding the relationship between Neo-Kantianism and other philosophical movements can help us appreciate the richness and complexity of contemporary philosophical thought.