bluuebunny/crossref_metadata_2025_split huggingface dataset:
Experimental Study on Parameters Optimization of Selective Laser Melting 316L Stainless Steel Based on the Response Surface Methodology
Authors: Wang Yali , Li Zhiyong , Cui Jingran , Zhang Wei , Liu Yuchen 
Published: April 2024

Abstract
The use of 3D printing technology can prepare flexible and varied special-shaped complex structures while realizing resource-saving and cost reduction. For this purpose, a 316L stainless steel sample was formed by selective laser melting technology, and the quality of samples was optimized by the Box–Behnken surface response method. Taking the surface roughness Sa as the response value, a regression analysis of four parameters of selective laser melting (laser power, scanning speed, scanning spacing, and scanning strategy) was designed using design expert software. The results showed that the scanning spacing and scanning speed have the greatest influence on the surface roughness, while the laser power and scanning strategy have no significant influence on the surface roughness. Meanwhile, the established surface roughness response surface model is effective and can be used for quality optimization of 316L structural trim. When the laser power was 185 W, the scanning speed was 615 mm/s, the scanning spacing was 110 μm and concentric scanning strategy was adopted, the surface adhesion powder was less, and the minimum surface roughness Sa was 9.001 μm.
Analysis of Gaps in Techno-Economic Analysis to Advance Heliostat Technologies for Concentrating Solar-Thermal Power
Authors: Augustine Chad , Zolan Alexander , Armijo Kenneth 
Published: April 2024

Abstract
The Heliostat Consortium (HelioCon) was launched in 2021 to advance heliostat technology. One of its first efforts was to do a detailed analysis of gaps in technology and capabilities in the heliostat industry and complete a roadmap study describing high-priority gaps. HelioCon gathered gaps through a series of outreach activities with representatives and experts from industries and research institutes. This paper discusses the gap analysis for the techno-economic analysis (TEA) topic. One of the main objectives of the TEA topic is to relate the cost and performance of heliostats and heliostat components to the overall system performance. In this study, we limit the scope of this topic to the heliostat field, tower, and receiver and do not consider downstream applications or uses of thermal energy. We conducted a thorough review of existing models and compiled a list of the state of the art in open-source tools currently available to researchers. We collected an initial list of gaps for the TEA of heliostats from industry developers and experts. Each gap is briefly described, and the heliostat development cycle stages that the gap impacts are indicated. We ranked the initial list of TEA gaps into tiers depending on their potential impact. For TEA, most of the gaps identified are related to developing models or data. Strictly speaking, none of these gaps are essential for heliostat development, but all would aid in the heliostat development process.
Development and performance evaluation of a qPCR-based assay for the fully automated detection of group B
Streptococcus
(GBS) on the Panther Fusion Open Access system
Authors: Caballero Méndez Andy , Reynoso de la Rosa Roberto A. , Abreu Bencosme Miguel E. , Sosa Ortiz Mayeline N. , Pichardo Beltré Eliezel , de la Cruz García Darah M. , Piñero Santana Nelson J. , Bacalhau de León Joana C. 
Published: April 2024

ABSTRACT

Streptococcus agalactiae
[group B
Streptococcus
(GBS)] poses a major threat as the primary cause of early-onset neonatal invasive disease, particularly when mothers are colonized rectovaginally. Although culture remains the gold standard for antepartum GBS screening, quantitative polymerase chain reaction (qPCR) offers advantages in terms of sensitivity and turnaround time. The aim of this study was to validate the clinical utility of an automated qPCR laboratory-developed test (LDT) for antepartum GBS screening using the Panther Fusion Open Access system (Hologic, California, USA). The LDT targeted a conserved region of the GBS
surface immunogenic protein
gene, demonstrating no cross-reactivity and high coverage (99.82%–99.99%). The limit of detection (LoD) was 118 CFU/mL. Comparison with commercial qPCR assays (Panther Fusion GBS and VIASURE
Streptococcus
B Real-Time) revealed an overall agreement of 99.7%, with a robust Cohen’s kappa coefficient of 0.992. Testing of 285 rectovaginal swabs from pregnant women and 15 external quality assessment samples demonstrated exceptional diagnostic performance of the LDT, achieving a diagnostic sensitivity and specificity of 100%, underscoring its accuracy. Prevalence and predictive values were also determined to reinforce test reliability. Our research highlights the limitations of culture-based screening and supports the suitability of our qPCR-based LDT for GBS detection in a clinical setting.

IMPORTANCE
Rectovaginal colonization by GBS is a major risk factor for early-onset invasive neonatal disease. The most effective approach to reducing the incidence of early-onset disease (EOD) has been described as universal screening, involving assessment of GBS colonization status in late pregnancy and intrapartum antibiotic prophylaxis. Despite its turnaround time and sensitivity limitations, culture remains the gold standard method for GBS screening. However, nucleic acid amplification-based tests, such as qPCR, have been utilized due to their speed and high sensitivity and specificity. This study validated the clinical usefulness of an automated qPCR-LDT for antepartum GBS screening through the Panther Fusion Open Access system (Hologic). Our study addresses the critical need for more robust, sensitive, and rapid strategies for GBS screening in pregnant women that could favorably impact the incidence of EOD.
Alzheimer’s disease-related presenilins are key to intestinal epithelial cell function and gut immune homoeostasis
Authors: Erkert Lena , Gamez-Belmonte Reyes , Kabisch Melanie , Schödel Lena , Patankar Jay V , Gonzalez-Acera Miguel , Mahapatro Mousumi , Bao Li-Li , Plattner Christina , Kühl Anja A , Shen Jie , Serneels Lutgarde , De Strooper Bart ,   TRR241 IBDome Consortium, Neurath Markus F , Wirtz Stefan , Becker Christoph 
Published: April 2024

ObjectiveMutations in presenilin genes are the major cause of Alzheimer’s disease. However, little is known about their expression and function in the gut. In this study, we identify the presenilins Psen1 and Psen2 as key molecules that maintain intestinal homoeostasis.DesignHuman inflammatory bowel disease (IBD) and control samples were analysed for Psen1 expression. Newly generated intestinal epithelium-specific Psen1-deficient, Psen2-deficient and inducible Psen1/Psen2 double-deficient mice were used to dissect the functional role of presenilins in intestinal homoeostasis.ResultsPsen1 expression was regulated in experimental gut inflammation and in patients with IBD. Induced deletion of Psen1 and Psen2 in mice caused rapid weight loss and spontaneous development of intestinal inflammation. Mice exhibited epithelial barrier disruption with bacterial translocation and deregulation of key pathways for nutrient uptake. Wasting disease was independent of gut inflammation and dysbiosis, as depletion of microbiota rescued Psen-deficient animals from spontaneous colitis development but not from weight loss. On a molecular level, intestinal epithelial cells lacking Psen showed impaired Notch signalling and dysregulated epithelial differentiation.ConclusionOverall, our study provides evidence that Psen1 and Psen2 are important guardians of intestinal homoeostasis and future targets for barrier-promoting therapeutic strategies in IBD.
Reactor neutrino physics potentials of cryogenic pure-CsI crystal
Authors: Wang Lei , Li Guanda , Yu Zeyuan , Liang Xiaohua , Wang Tian’an , Liu Fang , Sun Xilei , Guo Cong , Zhang Xin , Lei Yu , Chen Yuede 
Published: April 2024

AbstractThis paper presents a world-leading scintillation light yield among inorganic crystals measured from a 0.5 kg pure-CsI detector operated at 77 Kelvin. Scintillation photons were detected by two 2-inch Hamamatsu SiPM arrays equipped with cryogenic front-end electronics. Benefiting the light yield enhancement of pure-CsI at low temperatures and the high photon detection efficiency of SiPM, a light yield of 30.1 photoelectrons per keV energy deposit was obtained for X-rays and $$\gamma $$
γ
-rays with energies from 5.9 to 59.6 keV. Instrumental and physical effects in the light yield measurement are carefully analyzed. This is the first stable cryogenic operation of kg-scale pure-CsI crystal readout by SiPM arrays at liquid nitrogen temperatures for several days. The world-leading light yield opens a door for the usage of pure-CsI crystal in several fields, particularly in detecting the coherent elastic neutrino-nucleus scattering of reactor neutrinos. The potential of using pure-CsI crystals in neutrino physics is discussed in the paper.
A Modular Trial of Androgen Signaling Inhibitor Combinations Testing a Risk-Adapted Strategy in Patients with Metastatic Castration–Resistant Prostate Cancer
Authors: Aparicio Ana M. , Tidwell Rebecca S.S. , Yadav Shalini S. , Chen Jiun-Sheng , Zhang Miao , Liu Jingjing , Guo Shuai , Pilié Patrick G. , Yu Yao , Song Xingzhi , Vundavilli Haswanth , Jindal Sonali , Zhu Keyi , Viscuse Paul V. , Lebenthal Justin M. , Hahn Andrew W. , Soundararajan Rama , Corn Paul G. , Zurita-Saavedra Amado , Subudhi Sumit K. , Zhang Jianhua , Wang Wenyi , Huff Chad , Troncoso Patricia , Allison James P. , Sharma Padmanee , Logothetis Christopher J. 
Published: April 2024

Abstract

Purpose:
To determine the efficacy and safety of risk-adapted combinations of androgen signaling inhibitors and inform disease classifiers for metastatic castration–resistant prostate cancers.

Patients and Methods:
In a modular, randomized phase II trial, 192 men were treated with 8 weeks of abiraterone acetate, prednisone, and apalutamide (AAPA; module 1) and then allocated to modules 2 or 3 based on satisfactory (≥50% PSA decline from baseline and &lt;5 circulating tumor cell/7.5 mL) versus unsatisfactory status. Men in the former were randomly assigned to continue AAPA alone (module 2A) or with ipilimumab (module 2B). Men in the latter group had carboplatin + cabazitaxel added to AAPA (module 3). Optional baseline biopsies were subjected to correlative studies.

Results:
Median overall survival (from allocation) was 46.4 [95% confidence interval (CI), 39.2–68.2], 41.4 (95% CI, 33.3–49.9), and 18.7 (95% CI, 14.3–26.3) months in modules 2A (n = 64), 2B (n = 64), and 3 (n = 59), respectively. Toxicities were within expectations. Of 192 eligible patients, 154 (80.2%) underwent pretreatment metastatic biopsies. The aggressive-variant prostate cancer molecular profile (defects in ≥2 of p53, RB1, and PTEN) was associated with unsatisfactory status. Exploratory analyses suggested that secreted phosphoprotein 1–positive and insulin-like growth factor–binding protein 2–positive macrophages, druggable myeloid cell markers, and germline pathogenic mutations were enriched in the unsatisfactory group.

Conclusions:
Adding ipilimumab to AAPA did not improve outcomes in men with androgen-responsive metastatic castration–resistant prostate cancer. Despite the addition of carboplatin + cabazitaxel, men in the unsatisfactory group had shortened survivals. Adaptive designs can enrich for biologically and clinically relevant disease subgroups to contribute to the development of marker-informed, risk-adapted therapy strategies in men with prostate cancer.
Optimization of Absolute Coronary Blood Flow Measurements to Assess Microvascular Function: In Vivo Validation of Hyperemia and Higher Infusion Speeds
Authors: Minten Lennert , Bennett Johan , McCutcheon Keir , Oosterlinck Wouter , Algoet Michiel , Otsuki Hisao , Takahashi Kuniaki , Fearon William F. , Dubois Christophe 
Published: April 2024

BACKGROUND:
Reliable assessment of coronary microvascular function is essential. Techniques to measure absolute coronary blood flow are promising but need validation. The objectives of this study were: first, to validate the potential of saline infusion to generate maximum hyperemia in vivo. Second, to validate absolute coronary blood flow measured with continuous coronary thermodilution at high (40–50 mL/min) infusion speeds and asses its safety.

METHODS:
Fourteen closed-chest sheep underwent absolute coronary blood flow measurements with increasing saline infusion speeds at different dosages under general anesthesia. An additional 7 open-chest sheep underwent these measurements with epicardial Doppler flow probes. Coronary flows were compared with reactive hyperemia after 45 s of coronary occlusion.

RESULTS:

Twenty milliliters per minute of saline infusion induced a significantly lower hyperemic coronary flow (140 versus 191 mL/min;
P
=0.0165), lower coronary flow reserve (1.82 versus 3.21;
P
≤0.0001), and higher coronary resistance (655 versus 422 woods units;
P
=0.0053) than coronary occlusion. On the other hand, 30 mL/min of saline infusion resulted in hyperemic coronary flow (196 versus 192 mL/min;
P
=0.8292), coronary flow reserve (2.77 versus 3.21;
P
=0.1107), and coronary resistance (415 versus 422 woods units;
P
=0.9181) that were not different from coronary occlusion. Hyperemic coronary flow was 40.7% with 5 mL/min, 40.8% with 10 mL/min, 73.1% with 20 mL/min, 102.3% with 30 mL/min, 99.0% with 40 mL/min, and 98.0% with 50 mL/min of saline infusion when compared with postocclusive hyperemic flow. There was a significant bias toward flow overestimation (Bland-Altman: bias±SD, −73.09±30.52; 95% limits of agreement, −132.9 to −13.27) with 40 to 50 mL/min of saline. Occasionally, ischemic changes resulted in ventricular fibrillation (9.5% with 50 mL/min) at higher infusion rates.

CONCLUSIONS:
Continuous saline infusion of 30 mL/min but not 20 mL/min induced maximal hyperemia. Absolute coronary blood flow measured with saline infusion speeds of 40 to 50 mL/min was not accurate and not safe.
CAVIN1-Mediated hERG Dynamics: A Novel Mechanism Underlying the Interindividual Variability in Drug-Induced Long QT
Authors: Al Sayed Zeina R. , Pereira Céline , Le Borgne Rémi , Viaris de Lesegno Christine , Jouve Charlène , Pénard Esthel , Mallet Adeline , Masurkar Nihar , Loussouarn Gildas , Verbavatz Jean-Marc , Lamaze Christophe , Trégouët David-Alexandre , Hulot Jean-Sébastien 
Published: April 2024

BACKGROUND:

Drug-induced QT prolongation (diLQT) is a feared side effect that could expose susceptible individuals to fatal arrhythmias. The occurrence of diLQT is primarily attributed to unintended drug interactions with cardiac ion channels, notably the hERG (human ether-a-go-go-related gene) channels that generate the delayed-rectifier potassium current (I
Kr
) and thereby regulate the late repolarization phase. There is an important interindividual susceptibility to develop diLQT, which is of unknown origin but can be reproduced in patient-specific induced pluripotent stem cell–derived cardiomyocytes (iPS-CMs). We aimed to investigate the dynamics of hERG channels in response to sotalol and to identify regulators of the susceptibility to developing diLQT.

METHODS:

We measured electrophysiological activity and cellular distribution of hERG channels after hERG blocker treatment in iPS-CMs derived from patients with highest sensitivity (HS) or lowest sensitivity (LS) to sotalol administration in vivo (ie, on the basis of the measure of the maximal change in QT interval 3 hours after administration). Specific small interfering RNAs and CAVIN1-T2A-GFP adenovirus were used to manipulate
CAVIN1
expression.

RESULTS:

Whereas HS and LS iPS-CMs showed similar electrophysiological characteristics at baseline, the late repolarization phase was prolonged and I
Kr
significantly decreased after exposure of HS iPS-CMs to low sotalol concentrations. I
Kr
reduction was caused by a rapid translocation of hERG channel from the membrane to the cytoskeleton-associated fractions upon sotalol application.
CAVIN1
, essential for caveolae biogenesis, was 2× more highly expressed in HS iPS-CMs, and its knockdown by small interfering RNA reduced their sensitivity to sotalol.
CAVIN1
overexpression in LS iPS-CMs using adenovirus showed reciprocal effects. We found that treatment with sotalol promoted translocation of the hERG channel from the plasma membrane to the cytoskeleton fractions in a process dependent on CAVIN1 (caveolae associated protein 1) expression.
CAVIN1
silencing reduced the number of caveolae at the membrane and abrogated the translocation of hERG channel in sotalol-treated HS iPS-CMs. CAVIN1 also controlled cardiomyocyte responses to other hERG blockers, such as E4031, vandetanib, and clarithromycin.

CONCLUSIONS:
Our study identifies unbridled turnover of the potassium channel hERG as a mechanism supporting the interindividual susceptibility underlying diLQT development and demonstrates how this phenomenon is finely tuned by CAVIN1.
The Neural Corelates of Constructing Conceptual and Perceptual Representations of Autobiographical Memories
Authors: Gurguryan Lauri , Fenerci Can , Ngo Nguyet , Sheldon Signy 
Published: April 2024

Abstract
Contemporary neurocognitive frameworks propose that conceptual and perceptual content of autobiographical memories—personal past experiences—are processed by dissociable neural systems. Other work has proposed a central role of the anterior hippocampus in initially constructing autobiographical memories, regardless of the content. Here, we report on an fMRI study that utilized a repeated retrieval paradigm to test these ideas. In an MRI scanner, participants retrieved autobiographical memories at three timepoints. During the third retrieval, participants either shifted their focus to the conceptual content of the memory, the perceptual content of the memory, or retrieved the memory as they had done so on previous trials. We observed stronger anterior hippocampal activity for the first retrieval compared with later retrievals, regardless of whether there was a shift in content in those later trials. We also found evidence for separate cortical systems when constructing autobiographical memories with a focus on conceptual or perceptual content. Finally, we found that there was common engagement between later retrievals that required a shift toward conceptual content and the initial retrieval of a memory. This final finding was explored further with a behavioral experiment that provided evidence that focusing on conceptual content of a memory guides memory construction, whereas perceptual content adds precision to a memory. Together, these findings suggest there are distinct content-oriented cortical systems that work with the anterior hippocampus to construct representations of autobiographical memories.
Stopping Speed in Response to Auditory and Visual Stop Signals Depends on Go Signal Modality
Authors: Weber Simon , Salomoni Sauro E. , St George Rebecca J. , Hinder Mark R. 
Published: April 2024

Abstract
Past research has found that the speed of the action cancellation process is influenced by the sensory modality of the environmental change that triggers it. However, the effect on selective stopping processes (where participants must cancel only one component of a multicomponent movement) remains unknown, despite these complex movements often being required as we navigate our busy modern world. Thirty healthy adults (mean age = 31.1 years, SD = 10.5) completed five response-selective stop signal tasks featuring different combinations of “go signal” modality (the environmental change baring an imperative to initiate movement; auditory or visual) and “stop signal” modality (the environmental change indicating that action cancellation is required: auditory, visual, or audiovisual). EMG recordings of effector muscles allowed detailed comparison of the characteristics of voluntary action and cancellation between tasks. Behavioral and physiological measures of stopping speed demonstrated that the modality of the go signal influenced how quickly participants cancelled movement in response to the stop signal: Stopping was faster in two cross-modal experimental conditions (auditory go – visual stop; visual go – auditory stop), than in two conditions using the same modality for both signals. A separate condition testing for multisensory facilitation revealed that stopping was fastest when the stop signal consisted of a combined audiovisual stimulus, compared with all other go–stop stimulus combinations. These findings provide novel evidence regarding the role of attentional networks in action cancellation and suggest modality-specific cognitive resources influence the latency of the stopping process.
The Role of Higher Education in Science and Technology in the Development of Technology-Intensive Industries in China
Authors: Ryu Jiwon , Mah Jai S. 
Published: April 2024

Abstract
Technology-intensive industries in China have developed rapidly since its economic reform. This article examines the role of higher education institutions (HEI s) in science and technology (S&T) in innovation and the development of technology-intensive industries in China. First, it illustrates the evolution and current status of S&T education at HEI s and their relationship to enterprises and science parks that led to China’s innovation and technological development. Second, it reveals the government’s role in promoting S&T education at HEI s and strengthening the relationship between universities and enterprises. Finally, it derives policy implications for developing countries in light of China’s experience.
Cybersecurity Challenges in Indonesia: Threat and Responses Analysis
Authors: Pratiwi Fadhila Inas , Hennida Citra , Soesilowati Sartika , Berliantin Natavia , Ekasari Devi Yuni , Dewi Citra Sari , Intan Angelique Angie 
Published: April 2024

Abstract
The purpose of this research is to know the cyberthreat faced by Indonesian society and understand how the government responds towards this threat. This study employs two types of data. The first came from the literature for building the theoretical linkage, and the second came from an online survey of 328 respondents about cyberthreats that they experience, as well as the respondents’ perception regarding the government’s response. The results were then analyzed systematically along with the literature review analysis. The results show that 64 percent of Indonesian respondents have modest knowledge in terms of cyberthreats and regulations made for it. There are three main arguments for this condition. First, low level of knowledge in cyberthreat within the society. Second, Indonesian people unprepared to face the cyberthreat surrounding their daily life. Third, an inadequate Indonesian government response to prevent and tackle the cyberthreat.
Spinal Intramedullary Cysticercosis- A Case Report with Brief Review of Literature
Authors: Prachi  , Aiyer Hema Malini , Shrivastav Ashish Kumar , Sharma Gaurav 
Published: April 2024

Introduction: Although neurocysticercosis is the most common parasitic infestation in central nervous system, yet the intramedullary spinal involvement remains the extremely rare site of presentation. Due to its extreme rarity, these tumors pose diagnostic and therapeutic challenges and may be interpreted as intramedullary tumors. Hence to avoid this conundrum, a detailed clinical, radiological, and histopathological evaluation is mandatory to come to a definitive diagnosis. Case Report: We, hereby report a rare case of spinal intramedullary cysticercosis in a 19-year-old male, who was misdiagnosed as tuberculosis on imaging and was proven to be a case of neurocysticercosis with the aid of histopathology. Conclusion: The mainstay of lesion is complete surgical removal, followed by a mandate for detailed histopathological examination for early and appropriate diagnosis and management.