bluuebunny/crossref_metadata_2025_split huggingface dataset:
Complete genome sequence of
Tornadovirus japonicus
, a relative of Pacmanvirus, isolated from the Tamagawa River in Japan
Authors: Waschestjuk Daniel , Murata Kazuyoshi , Takemura Masaharu 
Published: June 2024

ABSTRACT

Here, we report the isolation and genome sequencing of a new Pacmanvirus-related isolate,
Tornadovirus japonicus
, from the Tamagawa River in Japan. This icosahedral virus has a genome of approximately 380 kb and 465 open reading frames, including two tRNA genes. The name “tornado” is based on its morphological features revealed by transmission electron microscopy analysis.
Complete genome sequence of the
Exiguobacterium
bacteriophage
Authors: Camacho-Beltrán Erika , Morales-Aguilar Juan José , López-Meyer Melina , Rincón-Enríquez Gabriel , Quiñones-Aguilar Evangelina Esmeralda 
Published: June 2024

ABSTRACT

We purified a lytic bacteriophage from soil collected in Guasave, Sinaloa: phiExGM16. This bacteriophage was isolated using the host,
Exiguobacterium acetilycum
. Its 17.6 kb genome contains 33 putative genes and shows a cover of 64% with 76.37% of nucleotide identity to
Microbacterium phage
Noelani.
Supplemental Material: Determining the age and origin of a Tertiary karstic system by in situ U-Pb geochronology on speleothems
Authors: Moreau Kevin , al. et 
Published: June 2024

<p>Supplemental text, Figures S1 to S15 (field samples, thin-sections, and isochrons), Table S1 (synthesis of U-Pb dating results), and Table S2 (metadata for LA-ICP-MS U-Pb ages of calcite). </p>
Replication of a local record keeping method for collecting road crash data in low resource settings: lessons from Bangladesh and Nepal
Authors: Thierry Martijn , Khadka Anish , Uddin Kazi Burhan , Parkin John , Rahman AKM Fazlur , Joshi Sunil Kumar , Mytton Julie A 
Published: June 2024

BackgroundPolice road crash and injury data in low-income and middle-income countries are known to under-report crashes, fatalities and injuries, especially for vulnerable road users. Local record keepers, who are members of the public, can be engaged to provide an additional source of crash and injury data.MethodsThis paper compares the application of a local record keeper method to capture road crash and injury data in Bangladesh and Nepal, assesses the quality of the data collected and evaluates the replicability and value of the methodology using a framework developed to evaluate the impact of being a local record keeper.OutcomeApplication in research studies in both Bangladesh and Nepal found the local record keeper methodology provided high-quality and complete data compared with local police records. The methodology was flexible enough to adapt to project and context differences. The evaluation framework enabled the identification of the challenges and unexpected benefits realised in each study. This led to the development of an 11-step process for conducting road crash data collection using local record keepers, which is presented to facilitate replication in other settings.ConclusionData collected by local record keepers are a flexible and replicable method to understand the strengths and limitations of existing police data, adding to the evidence base and informing local and national decision-making. The method may create additional benefits for data collectors and communities, help design and assess road safety interventions and support advocacy for improved routine police data.
Management and prevention strategies for osteoarthritis in tactical athletes
Authors: O'Sullivan Oliver 
Published: June 2024

Osteoarthritis (OA) affects over 600 million worldwide, is one of the leading causes of disability and has a significant burden of morbidity. There are multiple modifiable and non-modifiable risk factors, with professional and tactical athletes at higher risk than other occupational groups. Without specific anti-OA pharmacological agents, clinicians may feel helpless. However, primary, secondary and tertiary preventative strategies can slow or prevent OA development or progression. There are many modifiable risk factors which, if targeted, can contribute to an improvement in the experience of people living with OA. Radiological features of OA may signify the presence of ‘the disease’; however, the pain and symptoms experienced may be more accurately described as ‘the illness’. Targeting both, using a combination of the medical and biopsychosocial models of care, will improve the overall experience.This paper outlines some easily adoptable general and specific strategies to help manage this common and disabling condition, focused on improving joint healthspan, not just joint lifespan. They include education and communication, empowering individuals to confidently self-manage their condition with access to healthcare resources when required. A holistic package, including support for sleep, diet and weight loss, physical activity and specific home-based exercise routines, with appropriate analgesia when needed, can all improve OA illness and potentially slow OA disease development or progression. Clinicians should feel confident that there are many opportunities to intervene and mitigate the risk factors of OA, using various preventative strategies, especially in a young, physically active population with functional occupational or recreational demands.
Impact of product-based e-cigarette marketing on the attitudes and behavioural intentions of young Australians: an experimental study
Authors: Jongenelis Michelle I , McCausland Kahlia , Bode Stefan , Howard Tess , Ledger Melissa , Durkin Sarah J 
Published: June 2024

BackgroundThe tobacco industry has a history of using language to downplay the harms associated with cigarettes and mislead consumers and policymakers. Emerging evidence suggests similar tactics are being used in the context of e-cigarettes; however, exploration of the impact of product name on attitudes towards e-cigarettes and susceptibility to use is lacking. This experimental study explored whether attitudes towards e-cigarettes and susceptibility to use are influenced by the names used by the industry to describe and market these products.MethodAn accredited web panel provider recruited a sample of 383 Australians aged 12–29 years who had never smoked to participate in an online survey that featured an embedded experiment. Participants were randomly allocated to one of three conditions, each of which used a different name to describe e-cigarettes (condition 1: ‘e-cigarettes’, condition 2: ‘vapes’; condition 3: either ‘IGETS’, ‘Puff Bars’, ‘HQD Cuvies’ or ‘Gunnpods’). The survey assessed respondents’ overall opinion of the product described; attitudes towards the product; liking of the product; and curiosity, willingness and intentions to use the product.ResultsThose in the ‘brand name’ condition scored higher than those in the ‘e-cigarettes’ condition on all dependent variables. Those in the ‘vapes’ condition scored higher than those in the ‘e-cigarettes’ condition on product attitude.ConclusionFindings indicate that the use of brand names and terms such as ‘vapes’ instead of ‘e-cigarettes’ results in more favourable attitudes towards e-cigarettes and susceptibility to use among young Australians. Results highlight the problematic influence of promotional language use favoured by industry.
CDK4/6 Alters TBK1 Phosphorylation to Inhibit the STING Signaling Pathway in Prostate Cancer
Authors: Li Wei , Guo Feng , Zeng Ruijiang , Liang Huaiyuan , Wang Yinhuai , Xiong Wei , Wu Heshui , Yang Chunguang , Jin Xin 
Published: June 2024

Abstract
The efficacy of immunotherapy in patients with prostate cancer is limited due to the “cold” tumor microenvironment and the paucity of neoantigens. The STING-TBK1-IRF3 signaling axis is involved in innate immunity and has been increasingly recognized as a candidate target for cancer immunotherapy. Here, we found that treatment with CDK4/6 inhibitors stimulates the STING pathway and enhances the antitumor effect of STING agonists in prostate cancer. Mechanistically, CDK4/6 phosphorylated TBK1 at S527 to inactivate the STING signaling pathway independent of RB1 in prostate cancer cells. CDK4/6-mediated phosphorylation of RB1 at S249/T252 also induced the interaction of RB1 with TBK1 to diminish the phosphorylation of TBK1 at S172, which suppressed STING pathway activation. Overall, this study showed that CDK4/6 suppresses the STING pathway through RB1-dependent and RB1-independent pathways, indicating that CDK4/6 inhibition could be a potential strategy to overcome immunosuppression in prostate cancer.
Significance: Inhibiting CDK4/6 activates STING-TBK1-IRF3 signaling in prostate cancer by regulating TBK1 phosphorylation, suggesting that the combination of CDK4/6 inhibitors and STING agonists could be an effective approach to stimulate innate immunity.
Ku70 Binding to YAP Alters PARP1 Ubiquitination to Regulate Genome Stability and Tumorigenesis
Authors: Shu Yinyin , Jin Xiaoni , Ji Mintao , Zhang Zhisen , Wang Xiuxiu , Liang Haisheng , Lu Shuangshuang , Dong Shuai , Lin Yiping , Guo Yuhan , Zhuang Qiuyu , Wang Yuhong , Lei Zhe , Guo Lingchuan , Meng Xuanyu , Zhou Guangming , Zhang Wensheng , Chang Lei 
Published: June 2024

Abstract
Yes-associated protein (YAP) is a central player in cancer development, with functions extending beyond its recognized role in cell growth regulation. Recent work has identified a link between YAP/transcriptional coactivator with PDZ-binding motif (TAZ) and the DNA damage response. Here, we investigated the mechanistic underpinnings of the cross-talk between DNA damage repair and YAP activity. Ku70, a key component of the nonhomologous end joining pathway to repair DNA damage, engaged in a dynamic competition with TEAD4 for binding to YAP, limiting the transcriptional activity of YAP. Depletion of Ku70 enhanced interaction between YAP and TEAD4 and boosted YAP transcriptional capacity. Consequently, Ku70 loss enhanced tumorigenesis in colon cancer and hepatocellular carcinoma (HCC) in vivo. YAP impeded DNA damage repair and elevated genome instability by inducing PARP1 degradation through the SMURF2-mediated ubiquitin-proteasome pathway. Analysis of samples from patients with HCC substantiated the link between Ku70 expression, YAP activity, PARP1 levels, and genome instability. In conclusion, this research provides insight into the mechanistic interactions between YAP and key regulators of DNA damage repair, highlighting the role of a Ku70-YAP-PARP1 axis in preserving genome stability.
Significance: Increased yes-associated protein transcriptional activity stimulated by loss of Ku70 induces PARP1 degradation by upregulating SMURF2 to inhibit DNA damage, driving genome instability and tumorigenesis.
Response-locked theta dissociations reveal potential feedback signal following successful retrieval
Authors: Smith Devyn E. , Wheelock Justin R. , Long Nicole M. 
Published: June 2024

Abstract
Successful memory retrieval relies on memory processes to access an internal representation and decision processes to evaluate and respond to the accessed representation, both of which are supported by fluctuations in theta (4–8 Hz) activity. However, the extent to which decision-making processes are engaged following a memory response is unclear. Here, we recorded scalp electroencephalography (EEG) while human participants performed a recognition memory task. We focused on response-locked data, allowing us to investigate the processes that occur prior to and following a memory response. We replicate previous work and find that prior to a memory response theta power is greater for identification of previously studied items (hits) relative to rejection of novel lures (correct rejections; CRs). Following the memory response, the theta power dissociation “flips” whereby theta power is greater for CRs relative to hits. We find that the post-response “flip” is more robust for hits that are committed quickly, potentially reflecting a positive feedback signal for strongly remembered experiences. Our findings suggest that there are potentially distinct processes occurring before and after a memory response that are modulated by successful memory retrieval.
Enhancement of brain atlases with laminar coordinate systems: Flatmaps and barrel column annotations
Authors: Bolaños-Puchet Sirio , Teska Aleksandra , Hernando Juan B. , Lu Huanxiang , Romani Armando , Schürmann Felix , Reimann Michael W. 
Published: June 2024

Abstract
Digital brain atlases define a hierarchy of brain regions and their locations in three-dimensional Cartesian space, providing a standard coordinate system in which diverse datasets can be integrated for visualization and analysis. Although this coordinate system has well-defined anatomical axes, it does not provide the best description of the complex geometries of layered brain regions such as the neocortex. As a better alternative, we propose laminar coordinate systems that consider the curvature and laminar structure of the region of interest. These coordinate systems consist of a principal axis aligned to the local vertical direction and measuring depth, and two other axes that describe a flatmap, a two-dimensional representation of the horizontal extents of layers. The main property of flatmaps is that they allow a seamless mapping between 2D and 3D spaces through structured dimensionality reduction where information is aggregated along depth. We introduce a general method to define laminar coordinate systems and flatmaps based on digital brain atlases and according to user specifications. The method is complemented by a set of metrics to characterize the quality of the resulting flatmaps. We applied our method to two rodent atlases. First, to an atlas of rat somatosensory cortex based on Paxinos and Watson’s rat brain atlas, enhancing it with a laminar coordinate system adapted to the geometry of this region. Second, to the Allen Mouse Brain Atlas Common Coordinate Framework version 3, enhancing it with two flatmaps of the whole isocortex. We used one of these flatmaps to define new annotations of 33 individual barrels and barrel columns that are nonoverlapping and follow the curvature of the cortex, therefore, producing the most accurate atlas of mouse barrel cortex to date. Additionally, we introduced several applications highlighting the utility of laminar coordinate systems for data visualization and data-driven modeling. We provide a free software implementation of our methods for the benefit of the community.
Effect of different exercises on sleep quality in elderly women: a systematic review
Authors: Khaleghi M.M. , Ahmadi F. 
Published: June 2024

Abstract
Sleep disorder is a common problem in elderly women, which can cause problems and acute complications on society and the healthcare system, including reducing the quality of life. Exercise is a simple, low-cost, and effective way to improve sleep quality. The purpose of this study was to critically review the available literature on different types of physical activity that affect the sleep quality of elderly women to determine which type of physical activity can improve their sleep quality. A systematic search adhering to the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) guidelines and randomised controlled trial (RCT) studies that met the inclusion criteria was conducted in the databases of Google Scholar, PubMed (MEDLINE), Scopus, Embase, and Web of Science from January 1, 2020, to March 18, 2024. One hundred and thirty-nine articles were retrieved, of which 13 randomised controlled trial studies met the inclusion criteria. The findings of these studies showed that performing various exercises, including water sports, resistance, aerobics, Tai Chi, Qigong, and Baduanjin exercises across varying intensities from low to high, can improve the quality of sleep of elderly women. Research indicates that diverse exercise programs tailored for elderly women can effectively enhance sleep quality and its associated aspects.
The Role of Cloud-Radiative Interaction in Tropical Circulation and the Madden–Julian Oscillation
Authors: Huang Yuanyuan , Kim Daehyun , Zhou Tian , Shi Xiaoming 
Published: June 2024

Abstract
Cloud-radiative interaction (CRI) is a fundamental process that modulates tropical circulation and intraseasonal variability, including the Madden–Julian oscillation (MJO). In this study, we investigate how the mean state of the tropical atmosphere and the MJO respond to CRI intensity changes and provide insights into the underlying mechanisms, using the aquaplanet configuration in the Community Earth System Model, version 2 (CESM2). By enhancing CRI through tuning the DCS parameter (an autoconversion threshold size in the Morrison and Gettelman cloud microphysics scheme), we demonstrate that DCS-induced CRI intensification is linked to a warmer troposphere, increased tropical moisture, strengthened Hadley cell (HC), stronger trade winds, and a stronger equatorward intertropical convergence zone (ITCZ) with more clouds and precipitation, reflecting stronger cloud–radiation–circulation feedback. The intensified CRI also leads to the intensification and slower propagation of the simulated MJO-like mode despite the MJO-like signals becoming less distinguishable from the background due to the influence of other waves. The MJO intensification is likely associated with the mean state changes that support the development of deep convection. Moreover, the CRI itself, especially the interaction with the longwave radiation, also directly influences the MJO’s maintenance and propagation, more contributing to the maintenance of column moist static energy (MSE) and deceleration of its eastward propagation on intraseasonal time scales.
Geographical disparities in access to surgical treatment and mortality rates from abdominal aortic aneurysms in Brazil: A retrospective longitudinal study
Authors: Franco Rogério do Lago , Iora Pedro Henrique , Massago Miyoko , Arruda Beltrame Matheus Henrique , Hatoum Ualid Saleh , Giacomin Vinicius , Borba Isadora Martins , Belczak Sérgio Quilici , Staton Catherine Ann , Dutra Amanda de Carvalho , Andrade Luciano de 
Published: June 2024

Introduction: Abdominal aortic aneurysm (AAA) is a growing public health problem, and not all patients have access to surgery when needed. This study aimed to analyze spatiotemporal variations in AAA mortality and surgical procedures in Brazilian intermediate geographic regions and explore the impact of different surgical techniques on operative mortality. Methods: A retrospective longitudinal study was conducted to evaluate AAA mortality from 2008 to 2020 using space–time cube (STC) analysis and the emerging hot spot analysis tool through the Getis-Ord Gi* method. Results: There were 34,255 deaths due to AAA, 13,075 surgeries to repair AAA, and a surgical mortality of 14.92%. STC analysis revealed an increase in AAA mortality rates (trend statistic = +1.7693, p = 0.0769) and a significant reduction in AAA surgery rates (trend statistic = −3.8436, p = 0.0001). Analysis of emerging hotspots revealed high AAA mortality rates in the South, Southeast, and Central-West, with a reduction in procedures in São Paulo and Minas Gerais States (Southeast). In the Northeast, there were extensive areas of increasing mortality rates and decreasing procedure rates (cold spots). Conclusion: AAA mortality increased in several regions of the country while surgery rates decreased, demonstrating the need for implementing public health policies to increase the availability of surgical procedures, particularly in less developed regions with limited access to services.
Promoting child and adolescent health through wearable technology: A systematic review
Authors: Zhang Wei , Xiong Keying , Zhu Chengyan , Evans Richard , Zhou Lijuan , Podrini Christine 
Published: June 2024

Background Wearable technology is used in healthcare to monitor the health of individuals. This study presents an updated systematic literature review of the use of wearable technology in promoting child and adolescent health, accompanied by recommendations for future research. Methods This review focuses on studies involving children and adolescents aged between 2 and 18 years, regardless of their health condition or disabilities. Studies that were published from 2016 to 2024, and which met the inclusion criteria, were extracted from four academic databases (i.e. PubMed, Cochrane, Embase, and Web of Science) using the Preferred Reporting Items for Systematic Review and Meta-Analyses (PRISMA) protocol. Data on intervention purposes, interventions deployed, intervention duration, measurements, and the main outcomes of the studies were collected. Results A total of 53 studies involving 14,852 participants were reviewed. They focused on various aspects, including the ownership and use of wearable devices ( n = 3), the feasibility ( n = 22), effectiveness ( n = 4), and adherence ( n = 2) of intervention strategies, or a combination of multiple aspects ( n = 22). Among the interventions deployed, Fitbit was the most frequently used, featuring in 26 studies, followed by ActiGraph ( n = 11). In intervention studies, the majority of studies focused on pre-morbidity prevention ( n = 26) and the treatment of illnesses ( n = 20), with limited attention given to postoperative monitoring ( n = 4). Conclusions The use of wearable technology by children and adolescents has proven to be both feasible and effective for health promotion. This systematic review summarizes existing research by exploring the use of wearable technology in promoting health across diverse youth populations, including healthy and unhealthy individuals. It examines health promotion at various stages of the disease continuum, including pre-disease prevention, in-disease treatment, and postoperative monitoring. Additionally, the review provides directions for future research.
Does an allied health transdisciplinary stroke assessment save time, improve quality of care, and save costs? Results of a pre-/post- clinical study
Authors: Martin Aleysha K , Griffin Alison , McCarthy Alexandra L , Green Theresa L , Sowa P Marcin , Laakso E-Liisa 
Published: June 2024

Purpose: Demand for stroke services is increasing. To save time and costs, stroke care could be reorganised using a transdisciplinary assessment model embracing overlapping allied health professional skills. The study compares transdisciplinary assessment to discipline-specific allied health assessment on an acute stroke unit, by evaluating assessment time, quality of care, and cost implications. Method: The pre-/post- clinical study used non-randomised groups and 3-month follow-up after hospital admission. Patients with confirmed/suspected stroke received usual discipline-specific allied health assessment (pre-implementation phase) or the novel transdisciplinary assessment (post-implementation phase). Staff/student assessment times (primary outcome) and medical record data (secondary outcomes) were collected. Time differences were estimated using multivariable linear regression controlling for confounding factors. Cost minimisation and sensitivity analyses estimated change in hospital resource use. Findings: When the transdisciplinary assessment was used ( N = 116), compared to usual assessment ( N = 63), the average time saving was 37.6 min (95% CI −47.5, −27.7; p < 0.001) for staff and 62.2 min (95% CI −74.1, −50.3; p < 0.001) for students. The median number of allied health occasions of service reduced from 8 (interquartile range 4–23) to 5 (interquartile range 3–10; p = 0.011). There were no statistically significant or clinically important changes in patient safety, outcomes or stroke guideline adherence. Improved efficiency was associated with an estimated cost saving of $379.45 per patient (probabilistic 95% CI −487.15, −271.48). Discussion and conclusion: Transdisciplinary stroke assessment has potential for reorganising allied health services to save assessment time and reduce healthcare costs. The transdisciplinary stroke assessment could be considered for implementation in other stroke services.