bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sleep deprivation and recovery: Endurance racing as a novel model
Authors: Mann Dwayne L. , Pattinson Cassandra L , Allan Alicia , St Pierre Liam , Staton Sally , Thorpe Karen , Rossa Kalina , Smith Simon S. 
Published: June 2024

AbstractThe aim of this study was to investigate sleep‐wake behavior and gain insights into perceived impairment (sleep, fatigue, and cognitive function) of athletes competing in two international multi‐day adventure races. Twenty‐four athletes took part across two independent adventure races: Queensland, Australia and Alaska, USA. Individual sleep periods were determined via actigraphy, and racers self‐reported their perceived sleep disturbances, sleep impairment, fatigue and cognitive function. Each of these indices was calculated for pre‐, during‐ and post‐race periods. Sleep was severely restricted during the race period compared to pre‐race (Queensland, 7:46 [0:29] vs. 2:50 [1:01]; Alaska, 7:39 [0:58] vs. 2:45 [2:05]; mean [SD], hh:mm). As a result, there was a large cumulative sleep debt at race completion, which was not ‘reversed’ in the post‐race period (up to 1 week). The deterioration in all four self‐reported scales of perceived impairment during the race period was largely restored in the post‐race period. This is the first study to document objective sleep‐wake behaviors and subjective impairment of adventure racers, in the context of two geographically diverse, multi‐day, international adventure races. Measures of sleep deprivation indicate that sleep debt was extreme and did not recover to pre‐race levels within 1 week following each race. Despite this objective debt continuing, perceived impairment returned to pre‐race levels quickly post‐race. Therefore, further examination of actual and perceived sleep recovery is warranted. Adventure racing presents a unique scenario to examine sleep, performance and recovery.
Some hope for a dimensional assessment? A critical review of psychometric validated (semi‐)structured interview to assess eating disorders
Authors: Tournayre Lola , Balbinotti Marcos Alencar Abaide , Monthuy‐Blanc Johana 
Published: June 2024

AbstractObjectiveGiven that eating disorders (EDs) are considered one of the deadliest mental illnesses, the development of appropriate assessment instruments is a necessity. Despite the extensive literature on assessing EDs, there has been a lack of focus on semi‐structured interviews. The purpose of this article is to provide a comprehensive review of psychometrically validated semi‐structured interviews for EDs.MethodsIncluded studies (N = 24) were required to present a semi‐structured interview for EDs that has been validated through a psychometric process. The APA PsycNet, MEDLINE, APA Psycinfo, Pubmed, and Health & Psychosocial Instruments databases were searched. The literature search included publications through May 2024, with no earliest year restriction.ResultsA total of six instruments were identified and reviewed in terms of conceptual design, purpose and content, psychometric characteristics, and strengths and limitations. Three main findings were highlighted: (a) only half of the instruments are up to date; (b) the instruments are based on either a categorical or a mixed categorical‐dimensional approach; and (c) the predominance of the categorical approach.ConclusionsThe results are discussed regarding the conceptual approaches of the instrument to provide clinical and research implications. Despite the many strengths of the instrument, additional psychometric research is needed.
A hierarchical optimization technique for placement of battery energy storage system to improve grid transient stability
Authors: Shams Sabrina , Chowdhury Abdul H. , Islam Md. Minarul , Shafiullah Md. , Ustun Taha S. , Nahar Samsun , Muttaqi Kashem M. , Sutanto Danny 
Published: June 2024

AbstractA battery energy storage system (BESS), due to its very fast dynamic response, plays an essential role in improving the transient frequency stability of a grid. The performance of the BESS varies with the system's installation site. Hence, the optimal location of the BESS is of utmost importance for improving transient frequency stability. Therefore, this paper presents a hierarchical approach for optimizing the BESS placement to improve a grid's transient frequency stability. In most research, frequency nadir and rate of change of frequency (ROCOF) have been considered for studying frequency stability. This paper considers two more parameters, along with frequency nadir and ROCOF, to study the transient frequency stability, settling time, and decay ratio. A novel frequency stability index (FSI) using the four transient frequency parameters has been developed. After a significant disturbance in a benchmarked test system, the FSI was used to identify the optimal location of the BESS for stabilizing the frequency. It has been observed that, after a sudden generator outage, the ROCOF and the frequency nadir improve the best when the BESS is located at the bus closest to the generator experiencing the outage. However, considering the other two parameters as well, the value of the FSI is the minimum; that is, the optimum solution is when the BESS is located at the bus that is the second closest to the generator experiencing the outage. Results of similar studies validate the proposed FSI in indicating the optimal location of the BESS in improving the transient frequency behavior of the system.
Integration of artificial intelligence in clinical laboratory medicine: Advancements and challenges
Authors: Xie Heying , Jia Yin , Liu Shanrong 
Published: June 2024

AbstractArtificial intelligence (AI)‐driven analysis of comprehensive clinical parameters is bringing about a significant transformation in traditional routine clinical laboratory testing. This transformation impacts the prediction, prevention, diagnosis, and prognosis of human diseases. AI possesses the capability to efficiently analyze and process vast and intricate datasets, thereby facilitating the development of diverse and efficient diagnostic or predictive models. This advancement is fueling significant improvements in laboratory quality, automation, and the accuracy of diagnoses. In this context, we conducted a thorough review and discussion on the progression of AI applications in clinical laboratory medicine, encompassing advancements, implementation, and challenges. Our conclusion underscores that integrating AI into clinical laboratory testing will notably propel personalized precision medicine forward and enhance diagnostic accuracy, especially benefiting patients for whom accurate diagnoses are elusive through traditional laboratory testing systems.
Fighting fibrin with fibrin: Vancomycin delivery into coagulase‐mediated Staphylococcus aureus biofilms via fibrin‐based nanoparticle binding
Authors: Scull Grant , Aligwekwe Adrian , Rey Ysabel , Koch Drew , Nellenbach Kimberly , Sheridan Ana , Pandit Sanika , Sollinger Jennifer , Pierce Joshua G. , Flick Matthew J. , Gilbertie Jessica , Schnabel Lauren , Brown Ashley C. 
Published: June 2024

AbstractStaphylococcus aureus skin and soft tissue infection is a common ailment placing a large burden upon global healthcare infrastructure. These bacteria are growing increasingly recalcitrant to frontline antimicrobial therapeutics like vancomycin due to the prevalence of variant populations such as methicillin‐resistant and vancomycin‐resistant strains, and there is currently a dearth of novel antibiotics in production. Additionally, S. aureus has the capacity to hijack the host clotting machinery to generate fibrin‐based biofilms that confer protection from host antimicrobial mechanisms and antibiotic‐based therapies, enabling immune system evasion and significantly reducing antimicrobial efficacy. Emphasis is being placed on improving the effectiveness of therapeutics that are already commercially available through various means. Fibrin‐based nanoparticles (FBNs) were developed and found to interact with S. aureus through the clumping factor A (ClfA) fibrinogen receptor and directly integrate into the biofilm matrix. FBNs loaded with antimicrobials such as vancomycin enabled a targeted and sustained release of antibiotic that increased drug contact time and reduced the therapeutic dose required for eradicating the bacteria, both in vitro and in vivo. Collectively, these findings suggest that FBN‐antibiotic delivery may be a novel and potent therapeutic tool for the treatment of S. aureus biofilm infections.
Pyrene‐based ratiometric fluorescent chemosensor for sensitive detection of hypochlorite in a perfect aqueous solution
Authors: Kang Munseok , Lee Jae Jun , Gil Dongkyun , Lee Sooseong , Kim Cheal 
Published: June 2024

AbstractWe synthesized a pyrene‐based sensitive fluorescent chemosensor, TPA‐Cl ((E)‐N,N,N‐trimethyl‐2‐oxo‐2‐(2‐(pyren‐1‐ylmethylene)hydrazinyl)ethan‐1‐aminium chloride), to detect hypochlorite in a perfect aqueous solution. TPA‐Cl showed high selectivity for hypochlorite through fluorescence color change from sky blue to blue. The detection limit for hypochlorite was calculated as 0.27 μM, which is a quite low value. Moreover, the presence of other anions or ROS hardly hindered the sensing of hypochlorite by TPA‐Cl. Furthermore, TPA‐Cl could be applied successfully as a test kit for the simple recognition of hypochlorite. Surprisingly, TPA‐Cl is the first pyrene‐based fluorescent chemosensor that can detect ClO− by using the TPA‐Cl‐coated test kit. Moreover, TPA‐Cl could achieve the quantification of ClO− in various environmental water samples. The hydrolytic cleavage and oxidation mechanism of TPA‐Cl by hypochlorite was elucidated through UV–vis and fluorescent spectroscopy, 1H NMR titration, ESI‐MS analysis and DFT calculation.
Moving beyond symptom reduction: A meta‐analysis on the effect of exposure therapy for PTSD on quality of life
Authors: Kaur Kiran , Asnaani Anu , Levy Hannah C. , Miller Madeleine L. , Tolin David F. , McLean Carmen P. 
Published: June 2024

AbstractObjectivesQuality of life (QOL) is a multidimensional construct including emotional well‐being, life satisfaction, and physical health. Individuals with posttraumatic stress disorder (PTSD) consistently report low QOL, highlighting the importance of assessing the effectiveness of first‐line PTSD treatments (e.g., exposure‐based therapies) on QOL. This meta‐analysis examined the efficacy of exposure therapy for PTSD on QOL compared to control conditions (e.g., waitlist, medication, treatment‐as‐usual) at posttreatment and follow‐up (ranging from 1 month to 2 years).MethodsBuilding on a previous meta‐analysis of exposure‐based therapy for PTSD, we searched PsycINFO and Medline in December 2021, July 2022, and March 2023 to include randomized controlled trials of exposure‐based treatments for adult PTSD assessing QOL. We screened 295 abstracts for initial eligibility; 20 articles met inclusion criteria and were included (N = 2729 participants). Risk of bias was evaluated using the Cochrane Risk of Bias tool 2.0.ResultsAt posttreatment, exposure‐based therapies showed a medium effect on QOL relative to control conditions (k = 25, g = 0.67). This effect was not observed at follow‐up for the small subset of studies with follow‐up data (k = 8, g = 0.16). At posttreatment, effect size varied significantly as a function of the control condition (p < .0001). There were no differences in QOL effects across exposure therapies at posttreatment or follow‐up (p = .09).ConclusionExposure therapy was associated with greater improvement in QOL compared to control conditions at posttreatment. Exposure was not superior to control conditions at follow‐up, and the longer‐term impact of exposure on QOL is unclear. The implications of these findings are discussed, along with the need for more PTSD treatment studies to examine QOL outcomes at posttreatment and follow‐up.
Development of an efficient process to extract C‐phycocyanin from marine cyanobacterial strains and evaluation of its bioactive potential
Authors: Khazi Mahammed Ilyas , Demirel Zeliha , Liaqat Fakhra , Eltem Rengin , Conk Dalay Meltem 
Published: June 2024

AbstractBACKGROUNDPhycocyanin (PC), a natural blue photosynthetic pigment found in cyanobacteria, rhodophyta and cryptophyta has numerous applications in biotechnology and the food industry. The phycocyanin extraction method has a significant impact on its bioactivities, purity index, cost and potential applications; thus, the development of an efficient extraction strategy continues to be a primary research goal.RESULTSThis study evaluated the efficacy of different methods for the extraction of cyanobacterial phycocyanin (C‐PC) from three marine cyanobacterial isolates, namely Arthrospira platensis, Phormidium sp. and Pseudoscillatoria sp. wet biomass. The pretreatment step of soaking biomass in extraction buffer for 60 min, followed by a combination of freeze–thaw (one cycle; 2 h) and sonication (2 min) treatment was found to be the most effective extraction strategy, producing the highest C‐PC yield (16.19 ± 0.34%, 15.89 ± 0.61% and 15.56 ± 0.69% dry cell weight for strains A. platensis, Phormidium sp. and Pseudoscillatoria sp., respectively). Furthermore, a biomass‐to‐solvent ratio of 0.02 g mL−1 was optimal to attain food‐grade purity ((A620/A280) ≥ 0.7). Bioactivity evaluation of crude and purified C‐PC exhibited its potential as antioxidant compounds and as an antimicrobial agent with significant inhibitory effects against various pathogenic microorganisms.CONCLUSIONThe study proposed an efficient and cost‐effective C‐PC extraction process for cyanobacteria and highlighted the potential of C‐PC as a valuable bioactive compound with various applications. © 2024 Society of Chemical Industry (SCI).
The path forward for protein footprinting, covalent labeling, and mass spectrometry‐based protein conformational analyses
Authors: Borotto Nicholas B. 
Published: June 2024

AbstractMass spectrometry‐based approaches to assess protein conformation have become widely utilized due to their sensitivity, low sample requirements, and broad applicability to proteins regardless of size and environment. Their wide applicability and sensitivity also make these techniques suitable for the analysis of complex mixtures of proteins, and thus, they have been applied at the cell and even the simple organism levels. These works are impressive, but they predominately employ “bottom‐up” workflows and require proteolytic digestion prior to analysis. Once digested, it is not possible to distinguish the proteoform from which any single peptide is derived and therefore, one cannot associate distal—in primary structure—concurrent post‐translational modifications (PTMs) or covalent labels, as they would be found on separate peptides. Thus, analyses via bottom‐up proteomics report the average PTM status and higher‐order structure (HOS) of all existing proteoforms. Second, these works predominately employ promiscuous reagents to probe protein HOS. While this does lead to improved conformational resolution, the formation of many products can divide the signal associated with low‐copy number proteins below signal‐to‐noise thresholds and complicate the bioinformatic analysis of these already challenging systems. In this perspective, I further detail these limitations and discuss the positives and negatives of top‐down proteomics as an alternative.
Cytotoxic and viricidal effects of human semen on mumps virus‐infected lymphocytes: In vitro studies
Authors: Wang Fei , Shao Xinyi , Bao Binghao , Yang Yixuan , Wang Yu , Zhang Jing , Wang Siqi , Chen Yongmei , Han Daishu 
Published: June 2024

AbstractViruses in human semen may be sexually transmitted via free and cell‐mediated viral infection. The potential effects of semen on the infection and sexual transmission of most viruses in semen remain largely unclear. The present study elucidated the inhibitory effects of human seminal plasma (SP) on Jurkat cell (JC)‐mediated mumps virus (MuV) infection. We demonstrated that MuV efficiently infected JCs and that the JCs infected by MuV (JC‐MuV) mediated MuV infection of HeLa cells. Remarkably, SP was highly cytotoxic to JCs and inhibited JC‐MuV infection of HeLa cells. The cytotoxic factor possessed a molecular weight of less than 3 kDa, whereas that of the viricidal factor was over 100 kDa. The cooperation of cytotoxic and viricidal factors was required for the SP inhibition of JC‐MuV infection, and prostatic fluid (PF) was responsible for both the cytotoxic and viricidal effects of SP. The cytotoxic effects we observed were resistant to the treatment of PF with boiling water, proteinase K, RNase A, and DNase I. Our results provide novel insights into the antiviral properties of SP, which may limit cell‐mediated sexual viral transmission.
Survival analysis of teeth following clinical crown lengthening and crown insertion procedures up to 14 years: A retrospective cohort study
Authors: Oh Se‐Lim , Hu Jiaxin , Kwak Kee Hyun , Chung Man‐Kyo 
Published: June 2024

AbstractBackgroundThe purpose of this study was to conduct survival analysis of teeth following clinical crown lengthening procedures (CLPs) and crown insertions via a retrospective cohort study.MethodsPatient‐ and tooth‐related data were collected from 268 participants who received CLPs from 2009 to 2015. The Kaplan–Meier curve and the log‐rank tests were used to estimate the probability of survival and compare the survival probabilities among different variables. A Cox multivariate proportional hazard regression model was used to investigate the collective effects of root canal treatment (RCT) and the types of opposing dentition.ResultsThe rate of tooth loss was 21.6% during the observation period from 1 to 14 years, with 58 teeth extracted. The most attributable reason for tooth extraction was coronal tooth fracture, followed by endodontic failure such as root fracture. The survival probability was 0.87 at 5 years and 0.7 at 10 years. No significant differences in the survival probabilities were found among different providers and locations, the presence of a post, and the types of crowns. The hazard ratio for tooth loss was 6.3, 95% confidence interval (CI) [2.6 to 20.9] in the teeth with RCT (p < 0.001) and 2.4, 95% CI [1.1 to 4.8] in the teeth occluding implant‐retained prostheses (p = 0.016).ConclusionsTooth loss following CLPs and crown insertions appeared least among the teeth without RCT when occluding natural teeth, while tooth loss was most among the teeth with RCT when occluding implants.
The anti‐hyperplasia effect of polysaccharide from Prunella vulgaris L. on mammary gland hyperplasia in rats through regulation of the AKT‐FOXO3a signaling pathway and intestinal flora
Authors: Zhao Hong , Yang Yongyi , Zhou Yingming , Wen Han , Chen Chen , Li Changxu , Feng Yao , Li Lili , Li Xiaoliang 
Published: June 2024

AbstractBACKGROUNDPrunella vulgaris L., a medicinal and edible homologous plant, is often used to treat conditions such as breast hyperplasia, thyroid enlargement and lymphatic tuberculosis. Research has demonstrated that it is particularly effective in the treatment of mammary gland hyperplasia (MGH). However, the material basis and mechanism of its efficacy are still unclear.RESULTSOur results showed that in rats with MGH, polysaccharide from Prunella vulgaris L. (PVP) led to a reduction in the levels of estradiol, prolactin and malondialdehyde, while simultaneously increasing the concentrations of progesterone (P), superoxide dismutase (SOD), manganese superoxide dismutase (MnSOD) and catalase (CAT) in the serum. In addition, results obtained from 16S rRNA sequencing demonstrated that PVP had the capacity to increase the richness and diversity of the intestinal microbiota in MGH rats, as well as modify the structure of the microbiota. Correlation analysis revealed that the levels of P, SOD, MnSOD and CAT were positively associated with Allobaculum, Romboutsia, Faecalibaculum and Clostridium, while negatively correlated with Turicibacter.CONCLUSIONSThe mechanism of PVP in treating MGH might be through inhibiting the phosphorylation of the AKT‐FOXO3a signaling pathway and then activating the expression of downstream antioxidant enzymes, such as MnSOD and CAT. At the same time, PVP could restore intestinal flora homeostasis in rats with MGH by regulating the flora changes of Allobaculum, Romboutsia, Clostridium and Faecalibaculum, thereby reducing oxidative stress in rats with MGH. © 2024 Society of Chemical Industry.
Cover Image
Authors: Ji Jun , Yi Xiangzhou , Gao Xia , Wang Bohui , Zhang Xueying , Shen Xuanri , Xia Guanghua 
Published: June 2024

The cover image is based on the Research Article Synergistic effects of tilapia head protein hydrolysate and walnut protein hydrolysate on the amelioration of cognitive impairment in mice by Jun Ji et al., https://doi.org/10.1002/jsfa.13369.image
Assessing breast cancer‐related lymphedema screening and treatment gaps in a safety‐net hospital
Authors: Young Sara , Geary Alaina , Zhao Xuewei , Kenzik Kelly , Cassidy Michael , Newman Robin , Merrill Andrea L. 
Published: June 2024

AbstractBackground and ObjectivesWe evaluated the unmet breast cancer‐related lymphedema (BCRL) treatment and education needs at New England's largest safety‐net hospital serving a diverse population by assessing prevalence, risk factors, and treatment.MethodsThis was a retrospective cohort study examining breast cancer surgery patients from September 2016 to September 2021. The primary outcome was BCRL diagnosis. Secondary outcomes included BCRL risk factors, and physical and occupational therapy (PT/OT) referral frequency and completion.ResultsOf 639 patients, 17% of patients had documented BCRL, which was significantly associated with axillary lymph node dissection (ALND). There were no racial, insurance, breast radiation, or BMI category differences between patients with and without a BCRL diagnosis. Of those with BCRL, 58% received a PT/OT referral, and 56% completed their referral. There were no racial, insurance, or BMI category differences between those who received a PT/OT referral and those who did not.ConclusionIn our high‐risk population, rates of documented BCRL were higher than expected, approaching rates of ALND, despite the majority undergoing sentinel lymph node biopsy (SLNB). PT/OT referral and completion rates were low, concerning for an unmet BCRL treatment and education need. No disparities in care delivery were seen, but a program that addresses treatment gaps and ensures accessible and patient‐centered BCRL care is urgently needed.