bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sox11 regulates survival and axonal growth of embryonic sensory neurons
Authors: Lin L. , Lee V.M. , Wang Y. , Lin J.S. , Sock E. , Wegner M. , Lei L. 
Published: November 2010

AbstractSensory neurons transduce various stimuli including temperature, pain, and touch from the periphery to the central nervous system. Sensory neuron development is governed by a combination of extracellular cues and specific gene expression. We demonstrated that the transcription factor Sox11 was highly expressed in the developing sensory neurons. To test the function of Sox11, we used a knockin mouse model where the entire coding region of Sox11 was replaced by a LacZ reporter. The ablation of Sox11 caused severe reduction in sensory neuron survival in the trigeminal and dorsal root ganglia, although it did not affect migration of neural crest cells or acquisition of major sensory neuron subtypes. We further demonstrated that ablating Sox11 caused an arrest of axonal outgrowth in vivo and in vitro. This defect could not be fully rescued by blocking cell death. Our data suggest that Sox11 is a key regulator of sensory neuron development. Developmental Dynamics, 2011. © 2010 Wiley‐Liss, Inc.
Generation of a non‐leaky heat shock–inducible Cre line for conditional Cre/lox strategies in zebrafish
Authors: Hans Stefan , Freudenreich Dorian , Geffarth Michaela , Kaslin Jan , Machate Anja , Brand Michael 
Published: November 2010

AbstractCre‐mediated site‐specific recombination has emerged as an indispensable tool for the precise manipulation of the mammalian genome. Recently, we showed that Cre is also highly efficient in zebrafish and temporal control of recombination can be achieved by using the ligand‐inducible CreERT2. Previous attempts have been made to control recombination by using the temperature inducible hsp70l promoter to conditionally drive the expression of Cre or EGFP‐Cre, respectively. However, in this study we demonstrate that the hsp70l promoter possesses a basal leakiness resulting in Cre‐mediated recombination even at permissive temperatures. In order to prevent non‐conditional recombination, we combined the hsp70l promoter with a mCherry‐tagged ligand‐inducible CreERT2. At permissive temperatures and in the absence of the ligand tamoxifen (TAM), no non‐conditional recombination is observed indicating tight regulation of CreERT2. Instead, comprehensive site‐specific recombination is mediated following heat induction and administration of TAM. Developmental Dynamics, 2011. © 2010 Wiley‐Liss, Inc.
A basic study of optimal investment of power sources considering environmental measures: Economic evaluation of CCS through a real options approach
Authors: Kato Moritoshi , Zhou Yicheng 
Published: November 2010

AbstractThis paper proposes a novel method based on real options theory for economic evaluation of the replacement of an old coal‐fired thermal power plant (COAL) with a coal‐fired thermal power plant that includes a CCS (carbon dioxide capture and storage) unit. We calculate the real option value (ROV) of CCS as the value of an American call option with dividend, treating the increased carbon cost of COAL due to the delay of replacement as opportunity cost. The appropriate construction time of a CCS unit is evaluated by binominal decision tree analysis for option exercise. We calculate the change of project ROV and the appropriate construction time according to the difference in carbon emission benchmarks and the variation of the maintenance cost of COAL. The effect of power station lifetime is also analyzed. We conclude that flexibility in the timing of decision making increases the value of a project. We also found that CCS is an economically justifiable technology option under forthcoming restrictions on carbon emissions. © 2010 Wiley Periodicals, Inc. Electr Eng Jpn, 174(3): 9–17, 2011; Published online in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/eej.21065
Borrelia species induce inflammasome activation and IL‐17 production through a caspase‐1‐dependent mechanism
Authors: Oosting Marije , van de Veerdonk Frank L. , Kanneganti Thirumala‐Devi , Sturm Patrick , Verschueren Ineke , Berende Anneleen , van der Meer Jos W. M. , Kullberg Bart‐Jan , Netea Mihai G. , Joosten Leo A. B. 
Published: November 2010

AbstractBorrelia burgdorferi spirochetes cause Lyme disease, which can result in severe clinical symptoms such as multiple joint inflammation and neurological disorders. IFN‐γ and IL‐17 have been suggested to play an important role in the host defense against Borrelia, and in the immunopathology of Lyme disease. The caspase‐1‐dependent cytokine IL‐1β has been linked to the generation of IL‐17‐producing T cells, whereas caspase‐1‐mediated IL‐18 is crucial for IFN‐γ production. In this study, we show by using knockout mice the role of inflammasome‐activated caspase‐1 in the regulation of cytokine responses by B. burgdorferi. Caspase‐1‐deficient cells showed significantly less IFN‐γ and IL‐17 production after Borrelia stimulation. A lack of IL‐1β was responsible for the defective IL‐17 production, whereas IL‐18 was crucial for the IFN‐γ production. Caspase‐1‐dependent IL‐33 played no role in the Borrelia‐induced production of IL‐1β, IFN‐γ or IL‐17. In conclusion, we describe for the first time the role of the inflammasome‐dependent caspase‐1 activation of cytokines in the regulation of IL‐17 production induced by Borrelia spp. As IL‐17 has been implicated in the pathogenesis of chronic Lyme disease, these data suggest that caspase‐1 targeting may represent a new immunomodulatory strategy for the treatment of complications of late stage Lyme disease.
Threshold levels of Flt3‐ligand are required for the generation and survival of lymphoid progenitors and B‐cell precursors
Authors: Dolence Joseph J. , Gwin Kimberly , Frank Elena , Medina Kay L. 
Published: November 2010

AbstractThe generation of B‐cell precursors (BCP) from lymphohematopoietic progenitors (LHP) in bone marrow is dependent on signals provided by the receptor tyrosine kinase Flt3 and its ligand, Flt3‐ligand (FL). Mice deficient in FL exhibit striking reductions in LHP and BCP. Currently, the mechanism by which Flt3 regulates lymphoid lineage/B‐cell development is unknown. Here, we show that haploinsufficiency of FL (FL+/−) reduced the numbers of LHP, common lymphoid progenitors, and pro‐B cells, suggesting that FL levels set a threshold for B lymphopoiesis. Limiting dilution analysis confirmed reduced BCP frequency in FL+/− mice. Real‐time PCR of LHP from FL+/− animals showed increased transcripts of the B lineage inhibitor id1. However, targeted deletion of id1 did not restore the lymphoid/B lineage deficiencies in FL−/− mice, supporting Id1‐independent mechanisms. BrdU incorporation studies established that FL is not essential for the proliferation of Flt3+ multipotential progenitors. Analysis of FL−/− progenitors expressing low levels of Flt3 revealed decreased levels of the pro‐survival factor Mcl1. Consequently, the Flt3+ LHP progeny of Flt3low LSK+ cells exhibited increased Annexin V staining. Together, these data suggest that Flt3 signaling initiates a cascade of events in Flt3low precursors that promote the survival of LHP from which BCP are derived.
Synthesis and Structural Characterization of New Chiral (Biscarbene)platinum(II) Complexes
Authors: Riederer Stephanie K. U. , Bechlars Bettina , Herrmann Wolfgang A. , Kühn Fritz E. 
Published: November 2010

AbstractNew chiral bis(triazolium) salts have been prepared in good yields. The corresponding mononuclear platinum(II) complexes [Pt{bis(NHC)}X2] (X = Br, I) have been synthesized and spectroscopically characterized. One of the synthesized biscarbene complexes has also been characterised by X‐ray crystallography. These complexes are efficient catalyst precursors for the hydrosilylation of 1‐octene.
Ultra high performance liquid chromatography–mass spectrometric analysis of oxidized free fatty acids and acylglycerols
Authors: Tarvainen Marko , Suomela Jukka‐Pekka , Kallio Heikki 
Published: November 2010

AbstractA fast ultra high performance liquid chromatography (UHPLC)–electrospray ionization (ESI)–mass spectrometric (MS) method was developed for simultaneous analysis of free fatty acids (FFAs), monoacylglycerols (MAG), diacylglycerols (DAG), triacylglycerols (TAG), and their oxidized equivalents. Effect of elevated column temperature was studied in order to optimize the chromatography of closely eluting peaks and to reduce high back pressure formed in UHPLC. The elevated temperature enabled high flow rate, better mass transfer, and therefore more narrow peaks and better separation of the analytes. The new method was applied to the analysis of total lipid extracts of lipolysis samples prepared by an artificial digestion model in order to investigate oxidized lipids and changes in their profiles in the chyme. Over 150 compounds were identified from the extracts. The UHPLC–ESI–MS method was proved to be fast, highly selective, and sensitive. Compared to a previously used high performance LC–ESI–MS method, the new UHPLC–ESI–MS method was over five times faster and consumed one tenth of the solvents while producing comparable quantitative results.Practical applications: Edible oils and fats contain mainly TAGs, the lipolysis of which produces FFAs and MAGs with minor DAG components. These compounds are susceptible to oxidation in the stomach, and therefore the analysis of the oxidation products is important. Fast determination of FFAs and acylglycerols is also important in quality control of biodiesel. Our new method enables accurate and sensitive determination of different molecular species present in digested and processed samples with minimal sample preparation requirements. In this respect, the new method is applicable to large scale and fast screening of biological samples for lipidomic and metabolomic studies.
Integration profile of retroviral vector in gene therapy treated patients is cell‐specific according to gene expression and chromatin conformation of target cell
Authors: Biasco Luca , Ambrosi Alessandro , Pellin Danilo , Bartholomae Cynthia , Brigida Immacolata , Roncarolo Maria Grazia , Di Serio Clelia , von Kalle Christof , Schmidt Manfred , Aiuti Alessandro 
Published: November 2010

AbstractThe analysis of genomic distribution of retroviral vectors is a powerful tool to monitor ‘vector‐on‐host’ effects in gene therapy (GT) trials but also provides crucial information about ‘host‐on‐vector’ influences based on the target cell genetic and epigenetic state. We had the unique occasion to compare the insertional profile of the same therapeutic moloney murine leukemia virus (MLV) vector in the context of the adenosine deaminase‐severe combined immunodeficiency (ADA‐SCID) genetic background in two GT trials based on infusions of transduced mature lymphocytes (peripheral blood lymphocytes, PBL) or a single infusion of haematopoietic stem/progenitor cells (HSC). We found that vector insertions are cell‐specific according to the differential expression profile of target cells, favouring, in PBL‐GT, genes involved in immune system and T‐cell functions/pathways as well as T‐cell DNase hypersensitive sites, differently from HSC‐GT. Chromatin conformations and histone modifications influenced integration preferences but we discovered that only H3K27me3 was cell‐specifically disfavoured, thus representing a key epigenetic determinant of cell‐type dependent insertion distribution. Our study shows that MLV vector insertional profile is cell‐specific according to the genetic/chromatin state of the target cell both in vitro and in vivo in patients several years after GT.See accompanying Closeup by Baum DOI: http://dx.doi.org/10.1002/emmm.201000110.
Persistent resource allocations for VoIP traffic in packet‐switching mobile cellular networks
Authors: Sambale K. , Klagges K. , Rad R. Rezai 
Published: November 2010

AbstractIt is expected that future IMT‐Advanced systems will operate packet‐switched due to the dominance of data services. However, it is inherent to packet‐switched systems to not cope well with voice services as periodic resource usage patterns are not signalled efficiently. In this paper, we present a simple technique to reduce the signalling overhead for resource allocations especially for voice‐over‐IP (VoIP) traffic thus increasing the VoIP capacity of the system, considerably. The proposed technique assigns persistent resource allocation (PRA) per VoIP connection that are valid for a fix number of frames. Resource allocations for succeeding frames have to be signalled by new PRAs. This supersedes any procedures for deallocation of PRAs and associated error handling. Within our work we introduce an analytical model to evaluate the possible VoIP capacity gains. Additionally, we show results of event‐driven simulations to validate the analysis. The PRA technique is applicable to all packet‐switched systems. We show exemplary results for the WiMAX system. In this paper, we present a simple technique called persistent resource allocation (PRA) to reduce the signalling overhead in packet‐switching mobile radio networks, especially for VoIP traffic. The figure shows our idea: resource allocations for mobile station (MS) 42 are signalled in frame n (solid lines) and remain valid in frame $n + k$ up to frame $n + (m{-} 1)k$ (dashed lines). The parameter k matches the period length of packet arrival in number of frames and m limits the life time (time‐to‐live) of the PRA. Copyright © 2010 John Wiley & Sons, Ltd.
High‐resolution genomic screening in mantle cell lymphoma—specific changes correlate with genomic complexity, the proliferation signature and survival
Authors: Halldórsdóttir Anna M. , Sander Birgitta , Göransson Hanna , Isaksson Anders , Kimby Eva , Mansouri Mahmoud , Rosenquist Richard , Ehrencrona Hans 
Published: November 2010

AbstractMantle cell lymphoma (MCL) is characterized by the t(11;14)(q13;q32) and numerous copy number aberrations (CNAs). Recently, gene expression profiling defined a proliferation gene expression signature in MCL where high scores predict shorter survival. We investigated 31 MCL cases using high‐density single nucleotide polymorphism arrays and correlated CNA patterns with the proliferation signature and with clinical data. Many recurrent CNAs typical of MCL were detected, including losses at 1p (55%), 8p (29%), 9q (29%), 11q (55%), 13q (42%) and 17p (32%), and gains at 3q (39%), 8q (26%), 15q (23%) and 18q (23%). A novel deleted region at 20q (16%) contained only one candidate gene, ZFP64, a putative tumor suppressor. Unsupervised clustering identified subgroups with different patterns of CNAs, including a subset (19%) characterized by the presence of 11q loss in all cases and by the absence of 13q loss, and 3q and 7p gains. Losses at 1p, 8p, 13q and 17p were associated with increased genomic complexity. High proliferation signature scores correlated with increased number of large (>15 Mbp) CNAs (P = 0.03) as well as copy number gains at 7p (P = 0.02) and losses at 9q (P = 0.04). Furthermore, large/complex 13q losses were associated with improved survival (P < 0.05) as were losses/copy number neutral LOH at 19p13 (P = 0.01). In summary, this high‐resolution genomic analysis identified novel aberrations and revealed that several CNAs correlated with genomic complexity, the proliferation status and survival. © 2010 Wiley‐Liss,Inc.
Multiplexed immunobead‐based profiling of cytokine markers for detection of nasopharyngeal carcinoma and prognosis of patient survival
Authors: Chang Kai‐Ping , Chang Ya‐Ting , Wu Chih‐Ching , Liu Yu‐Ling , Chen Min‐Chi , Tsang Ngan‐Ming , Hsu Cheng‐Lung , Chang Yu‐Sun , Yu Jau‐Song 
Published: November 2010

AbstractBackgroundThe purpose of this study was to examine cytokine profiles of peripheral blood for nasopharyngeal carcinoma (NPC).MethodsA total of 132 patients with untreated NPC, 169 healthy individuals, and 32 patients with chronic rhinosinusitis (CRS) were enrolled in this study. The plasma levels of 13 cytokines were measured by the multiplexed immunobead‐based method.ResultsThe levels of interleukin (IL)‐6, IL‐8, interferon‐inducible protein 10 (IP‐10), tumor necrosis factor (TNF)‐α, vascular endothelial growth factor (VEGF), and macrophage inflammatory protein (MIP)‐3α were significantly elevated in patients with NPC; but the stem cell factor (SCF) levels were significantly lower. The levels of IL‐6, IL‐8, IP‐10, and MIP‐3α were significantly elevated in patients with advanced stages disease and correlated with Epstein–Barr virus (EBV) DNA. A 2‐marker panel consisting of MIP‐3α and SCF increased the screening efficacy of EBV‐specific viral capsid antigen (VCA) IgA or EBV DNA, although the addition of MIP‐3α and SCF to the combined set of currently available markers (EBV VCA IgA and EBV DNA) did not improve the accuracy of the diagnostic panel. The patients with NPC with higher levels of IL‐8, VEGF, MIP‐3α, and EBV DNA had worse prognoses for overall survival (p = .008, .035, .005, and .007, respectively).ConclusionSimultaneous, large‐scale measurement of multiple cytokines may improve NPC detection and prognostic prediction. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Endoscopic transoral oropharyngectomy using laparoscopic surgical instruments
Authors: Yamashita Taku , Tomifuji Masayuki , Araki Koji , Kurioka Takaomi , Shiotani Akihiro 
Published: November 2010

AbstractBackgroundRecently, several transoral approaches for minimally invasive surgery have been developed for oropharyngeal cancer. However, all approaches have certain disadvantages. Therefore, we built and examined new surgical environments for improving the situation.MethodsEndoscopic transoral resection using a pharyngeal retractor or distending laryngoscope, rigid videoendoscope, and laparoscopic surgical instruments was performed in 17 patients with oropharyngeal squamous cell carcinoma. Postoperative results regarding oncology and function as well as complications were documented.ResultsThe 2‐year relapse‐free survival rate was 100%. Postoperative swallowing and speech function were satisfactory: 94.1% of cases scored ≤1 on the functional outcome swallowing scale (FOSS) and ≤1 on the communication score. Further, no perioperative mortality occurred.ConclusionsThe improved surgical environment made it possible to perform a safe and reliable en bloc resection with a wide field of view and sufficient working space. Our system can be useful, although further studies on additional cases are required. Head Neck, 2010
Prognostic impact of different TNM‐based stage groupings for oral squamous cell carcinoma
Authors: Kreppel Matthias , Drebber Uta , Rothamel Daniel , Eich Hans‐Theodor , Kübler Alexander , Scheer Martin , Zöller Joachim E. 
Published: November 2010

AbstractBackgroundThe purpose of this study was to evaluate the prognostic significance of different TNM‐based stage groupings proposed in the literature.MethodsWe conducted a retrospective analysis of 300 patients with primary oral squamous cell carcinoma (T1–4, N0–2, M0). The stage grouping systems of the sixth edition of the Union Internationale Contre le Cancer (UICC), T and N Integer Score (TANIS), the Snyderman scheme, the Hart scheme, and the Berg scheme were tested for their prognostic significance. Disease free survival (DFS) was plotted by Kaplan–Meier analysis. Prognostic factors were identified through univariate and multivariate analysis.ResultsOn univariate analysis, all systems revealed discriminatory power for DFS; however, on multivariate analysis, only the Hart scheme predicted DFS. The TANIS did not have a better prognostic ability than the UICC stage grouping.ConclusionUnlike in previous studies, the UICC stage grouping did perform worse than other TNM‐based stage groupings, which may be due to the alterations made in the sixth edition. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Effect of poly(ethylene glycol) diacrylate concentration on network properties and in vivo response of poly(β‐amino ester) networks
Authors: Safranski David L. , Weiss Daiana , Clark J. Brian , Caspersen Birgitta S. , Taylor W. Robert , Gall Ken 
Published: November 2010

AbstractPoly(β‐amino ester) networks have shown promise as tissue scaffolds. The objective of this work was to examine the effect of changing poly(ethylene glycol) diacrylate concentration on poly(β‐amino ester) network properties and to assess the degradable polymers' in vivo response, using magnetic resonance imaging (MRI) and immunohistochemistry. The networks were synthesized from hexanediol diacrylate (HDDA), poly(ethylene glycol) diacrylate (PEGDA), and a primary amine, 3‐methoxypropylamine (3‐MOPA), with a fixed overall molar ratio of diacrylate to amine. Network properties were verified to insure that the networks possessed equivalent initial properties and structure other than chemistry. The effect of varying PEGDA concentration on water content, mass loss, and modulus was determined, where increasing the concentration of PEGDA increases both water content, mass loss rate, and decreases modulus. We also show that manipulating the network composition at ratios of 0:100, 10:90 and 25:75 (PEGDA:HDDA) does not elicit a major inflammatory response to subcutaneous implantation of the networks in mice. This work provides a foundation for tailoring poly(β‐amino ester) networks, based on degradation rate and modulus, as a means to tune the polymer properties for various biomedical applications. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
Bovine (Bos taurus) humoral immune response against Echinococcus granulosus and hydatid cyst infertility
Authors: Paredes Rodolfo , Godoy Pablo , Rodríguez Betsabé , García María Pía , Cabezón Carolina , Cabrera Gonzalo , Jiménez Verónica , Hellman Ulf , Sáenz Leonardo , Ferreira Arturo , Galanti Norbel 
Published: November 2010

AbstractEchinococcus granulosus, the agent of hydatid disease, presents an indirect life cycle, with canines (mainly dogs) as definitive hosts, and herbivores and human as intermediary ones. In intermediary hosts fertile and infertile cysts develop, but only the first ones develop protoscoleces, the parasite form infective to definitive hosts. We report the presence of bovine IgGs in the germinal layer from infertile cysts (GLIC), in an order of magnitude greater than in the germinal layer from fertile cysts (GLFC). When extracted with salt solutions, bovine IgGs from GLIC are associated with low or with high affinity (most likely corresponding to non specific and antigen specific antibodies, respectively). Specific IgGs penetrate both the cells of the germinal layer and HeLa cultured cells and recognize parasitic proteins. These results, taken together with previous ones from our laboratory, showing induction of apoptosis in the germinal layer of infertile hydatid cysts, provide the first coherent explanation of the infertility process. They also offer the possibility of identifying the parasite antigens recognized, as possible targets for immune modulation. J. Cell. Biochem. 112: 189–199, 2011. © 2010 Wiley‐Liss, Inc.