bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association Between Oral Malodor and Dementia: An 11-Year Follow-Up Study in Japan
Authors: Ho Duc Sy Minh , Zaitsu Takashi , Ihira Hikaru , Iwasaki Masanori , Yoshihara Akihiro , Suzuki Seitaro , Inoue Manami , Yamagishi Kazumasa , Yasuda Nobufumi , Aida Jun , Shinozaki Tomohiro , Goto Atsuhi , Tsugane Shoichiro , Sawada Norie 
Published: May 2024

Background: As infrequent social interaction is a potential risk of dementia, oral malodor may increase the risk of dementia, including Alzheimer’s disease. Objective: This study investigated the association between malodor and dementia. Methods: We used the Japan Public Health Center-based Prospective Study data obtained at Yokote City. A total of 1,493 individuals aged 56 to 75 years underwent a dental examination and self-reported survey from May 2005 to January 2006. Follow-up for the onset of dementia was conducted using long-term care insurance data from 2006 to 2016. Hazard ratios of oral malodor on dementia were estimated by the Cox proportional hazards model. The inverse probability-weighted Cox model was used as a sensitivity analysis. Results: The study comprised 1493 participants (53.6% women) with a mean age of 65.6 (SD = 5.8) years old; at the end of the follow-up, 6.4% ( n = 96) developed dementia, and the percentage was 20.7 in severe malodor group. Throughout 15274.133 person-years of follow-up, the average incidence rate for the onset of dementia per 1000 person-years was 6.29. The highest incidence rate was seen in participants with severe malodor (22.4 per 1000 person-years). After adjusting for confounders, compared to those with no malodor, there was a 3.8 (95% confidence interval: 1.5 to 9.4) times greater hazard of developing dementia in participants with severe malodor. The inverse probability weighted Cox model confirmed the same trend with an adjusted marginal hazard ratio of 4.4 (1.2 to 16.4). CONCLUSIONS: A significant association between oral malodor and the onset of dementia exists.
Validity and reliability of the Valkyria Trainer Balance® portable force platform in its isometric mode
Authors: Huerta Ojeda Álvaro , Beltrán-Inostroza Rodrigo , Yeomans-Cabrera María-Mercedes , Torres-Banduc Maximiliano 
Published: May 2024

BACKGROUND: Isometric maximal voluntary force (IMVF) is essential for individuals’ health and physical performance. Therefore, there is a need for valid and reliable devices to assess IMVF. OBJECTIVE: To determine the validity and reliability of the Valkyria Trainer Balance® portable force platform in its isometric mode. METHODS: Fifty-eight physically healthy individuals (30 men and 28 women) participated in the study. A repeated measures design was used to compare the inter-day test-retest reliability of peak force. The validity of the Valkyria Trainer Balance® force platform was determined by comparing the peak force with the ArtOficio® force platform. The analysis consisted of the intraclass correlation coefficient (ICC), standard error of measurement (SEM), and coefficient of variation (CV). A CV [Formula: see text] 10% and ICC [Formula: see text] 0.80 were considered acceptable reliability, while a [Formula: see text] 5% and ICC [Formula: see text] 0.90 were regarded as high reliability. RESULTS: CV the peak force showed high test-retest inter-day reliability (CV [Formula: see text] 4.3% and ICC [Formula: see text] 0.99). When comparing both force platforms, there was a 1.1% difference between the two devices. CONCLUSIONS: The results of this study demonstrate that the Valkyria Trainer Balance® force platform is valid and reliable for assessing IMVF in physically healthy individuals.
Apoptosis, Autophagy, and Mitophagy Genes in the CA3 Area in an Ischemic Model of Alzheimer’s Disease with 2-Year Survival
Authors: Pluta Ryszard , Bogucka-Kocka Anna , Bogucki Jacek , Kocki Janusz , Czuczwar Stanisław J. 
Published: May 2024

Background: Currently, no evidence exists on the expression of apoptosis ( CASP3), autophagy ( BECN1), and mitophagy ( BNIP3) genes in the CA3 area after ischemia with long-term survival. Objective: The goal of the paper was to study changes in above genes expression in CA3 area after ischemia in the period of 6–24 months. Methods: In this study, using quantitative RT-PCR, we present the expression of genes associated with neuronal death in a rat ischemic model of Alzheimer’s disease. Results: First time, we demonstrated overexpression of the CASP3 gene in CA3 area after ischemia with survival ranging from 0.5 to 2 years. Overexpression of the CASP3 gene was accompanied by a decrease in the activity level of the BECN1 and BNIP3 genes over a period of 0.5 year. Then, during 1-2 years, BNIP3 gene expression increased significantly and coincided with an increase in CASP3 gene expression. However, BECN1 gene expression was variable, increased significantly at 1 and 2 years and was below control values 1.5 years post-ischemia. Conclusions: Our observations suggest that ischemia with long-term survival induces neuronal death in CA3 through activation of caspase 3 in cooperation with the pro-apoptotic gene BNIP3. This study also suggests that the BNIP3 gene regulates caspase-independent pyramidal neuronal death post-ischemia. Thus, caspase-dependent and -independent death of neuronal cells occur post-ischemia in the CA3 area. Our data suggest new role of the BNIP3 gene in the regulation of post-ischemic neuronal death in CA3. This suggests the involvement of the BNIP3 together with the CASP3 in the CA3 in neuronal death post-ischemia.
Interprofessional collaboration mediates the relationship between perceived organizational learning and safety climate in hospitals: A cross-sectional study
Authors: Ishii Keiko , Fujitani Katsumi , Matsushita Hironobu 
Published: May 2024

BACKGROUND: Organizational learning (OL) and interprofessional collaboration (IPC) are said to enhance medical safety in hospitals, but the relationship between these variables has not been quantitatively tested. OBJECTIVE: This study examines the mediating effects of IPC on the relationship between OL and safety climate (improvement, compliance, and patient/family involvement). METHODS: An anonymous self-reporting questionnaire was administered to 1,495 healthcare workers from November 2021 to January 2022. The questions regarded the hospital’s safety climate, OL, and IPC. A mediation analysis using structural equation modeling was conducted to examine the mediating role of IPC on the relationship between OL and the three safety climates. The indirect effect was estimated using 2,000 bootstrap samples. RESULTS: Responses from 643 healthcare workers were analyzed. The direct effects of OL were 𝛽 = .74, 75 ( p < .001) on improvement and involvement and 𝛽 = 0.1 ( p > .05) on compliance. The indirect effects of IPC on improvement and involvement were 𝛽 = .14 (95%CI: .00 ∼ .06) and 𝛽 = .37 (95%CI: .04 ∼ .09), respectively. CONCLUSION: This study determined the mechanisms that enhance a hospital’s safety climate, demonstrating that IPC mediates the relationship between OL and improvement and patient/family involvement. However, OL and IPC are not related to compliance.
Gambaran Kemampuan Empati Mahasiswa Profesi Ners Fakultas Keperawatan Universitas Padjadjaran
Authors: Fitri Wiani Syawalia , Hana Rizmadewi Agustina , Aan Nuraeni 
Published: May 2024

Perawat merupakan garda terdepan dalam tatanan pelayanan kesehatan yang berperan memberikan pelayanan berkualitas terhadap kepuasan pasien. Kemampuan empati dibutuhkan untuk mengembangkan hubungan yang baik antara perawat dan pasien. Kemampuan empati mulai dikembangkan sejak mahasiswa menempuh pendidikan keperawatan. Penelitian ini bertujuan untuk mengetahui kemampuan empati mahasiswa Profesi Ners Fakultas Keperawatan Universitas Padjadjaran. Penelitian berjenis deskriptif kuantitatif, dilakukan kepada mahasiswa Profesi Ners angkatan 41 dan 42 (N=212) dengan teknik penarikan sampel total sampling. Peneliti menggunakan platform online untuk menyebarluaskan kuesioner Jefferson Scale of Physician Empathy - Nursing Student Version versi bahasa Indonesia yang telah diuji validitas dan reabilitasnya. Hasil penelitian menunjukkan bahwa mahasiswa Profesi Ners memiliki skor empati yang tinggi, dengan skor rata-rata 108,85 (SD = 13,17). Sebagian besar 59.44% mahasiswa menunjukkan kemampuan empati yang tinggi; sebagian besar peserta memperoleh nilai yang tinggi untuk komponen empati perspective-taking (52,36%), compassionate care (61,32%), dan standing in the patients' shoes (54,26%). Mengembangkan program pendidikan dapat merangsang kemampuan empati di kalangan mahasiswa keperawatan merupakan suatu hal yang wajib. Salah satunya melalui metode pembelajaran dengan simulasi caring dan empati.
Changes in walking function and neural control following pelvic cancer surgery with reconstruction
Authors: Li Geng , Ao Di , Vega Marleny M. , Zandiyeh Payam , Chang Shuo-Hsiu , Penny Alexander. N. , Lewis Valerae O. , Fregly Benjamin J. 
Published: May 2024

Introduction: Surgical planning and custom prosthesis design for pelvic cancer patients are challenging due to the unique clinical characteristics of each patient and the significant amount of pelvic bone and hip musculature often removed. Limb-sparing internal hemipelvectomy surgery with custom prosthesis reconstruction has become a viable option for this patient population. However, little is known about how post-surgery walking function and neural control change from pre-surgery conditions.Methods: This case study combined comprehensive walking data (video motion capture, ground reaction, and electromyography) with personalized neuromusculoskeletal computer models to provide a thorough assessment of pre- to post-surgery changes in walking function (ground reactions, joint motions, and joint moments) and neural control (muscle synergies) for a single pelvic sarcoma patient who received internal hemipelvectomy surgery with custom prosthesis reconstruction. Pre- and post-surgery walking function and neural control were quantified using pre- and post-surgery neuromusculoskeletal models, respectively, whose pelvic anatomy, joint functional axes, muscle-tendon properties, and muscle synergy controls were personalized using the participant’s pre-and post-surgery walking and imaging data. For the post-surgery model, virtual surgery was performed to emulate the implemented surgical decisions, including removal of hip muscles and implantation of a custom prosthesis with total hip replacement.Results: The participant’s post-surgery walking function was marked by a slower self-selected walking speed coupled with several compensatory mechanisms necessitated by lost or impaired hip muscle function, while the participant’s post-surgery neural control demonstrated a dramatic change in coordination strategy (as evidenced by modified time-invariant synergy vectors) with little change in recruitment timing (as evidenced by conserved time-varying synergy activations). Furthermore, the participant’s post-surgery muscle activations were fitted accurately using his pre-surgery synergy activations but fitted poorly using his pre-surgery synergy vectors.Discussion: These results provide valuable information about which aspects of post-surgery walking function could potentially be improved through modifications to surgical decisions, custom prosthesis design, or rehabilitation protocol, as well as how computational simulations could be formulated to predict post-surgery walking function reliably given a patient’s pre-surgery walking data and the planned surgical decisions and custom prosthesis design.
Short-term effects of understory removal on understory diversity and biomass of temperate forests in northeast China
Authors: Zhang Yanyan , Yuan Quan , Deng Jiaojiao , Zhou Li , Yu Dapao , Zhou Wangming , Wang Qing-Wei 
Published: May 2024

IntroductionUnderstory removal is a traditional practice in forest management to reduce fire risk and promote seedling regeneration. However, its effect on understory diversity, biomass and soil nutrients in temperate forest ecosystems is less known, which limits our assessment of the effectiveness of understory vegetation management.MethodsWe quantified the composition of the understory species, their diversity, and the biomass of the understory and factors driving changes in these parameters in primary mixed broad-leaved Pinus koraiensis forest (BKF), secondary Betula platyphylla forest (BF), and Larix gmelinii plantation (LF) in northeast China after a 5-year understory removal.ResultsAfter understory removal, the number of shrub and herb species in BKF and LF decreased, while the number of shrub species in BF increased significantly and that of herb species decreased; the species with strong light preference, Equisetum hyemale, Impatiens noli-tangere, and Filipendula Palmata, were dominant in the herb layer of the three forest types; Shannon–Wiener diversity, Pielou evenness, and Simpson diversity of the herb layer in LF increased significantly (P &lt; 0.05), while those of the shrub and herb layers in BF and LF showed no significant changes (P &gt; 0.05). The total understory biomass of understory of BKF and LF decreased by 0.94 t·hm−2 and 1.32 t·hm−2, respectively, while that of BF increased by 1.31 t·hm−2; soil NH4+-N and total phosphorus (TP) were the key factors regulating understory vegetation diversity and biomass, respectively.ConclusionThese results suggest that understory removal is a beneficial management strategy for increasing shrub biomass and diversity in secondary forests, while it should be avoided in primary forests and plantations to prevent the reduction of understory plant diversity and soil nutrient loss.
Characteristics of phosgene aspiration lung injury analyzed based on transcriptomics and proteomics
Authors: Wang Li-Na , Shao Yi-Ru , Wang Peng-Fei , Lv Jiang , He Dai-Kun 
Published: May 2024

BackgroundPhosgene is a chemical material widely used worldwide. No effective method has been developed to reverse its pathological injuries. Some studies have shown that neuronal inflammation in lung tissue is involved, but the specific mechanism has not been reported.ObjectiveTo analyze the expression alterations of whole transcriptome gene sequencing bioinformatics and protein expression profile in lung tissue after phosgene aspiration lung injury (P-ALI) and find the main factors and pathways affecting the prognosis of P-ALI.MethodsRat models of P-ALI were made by phosgene. Rats were divided into a P-ALI group and a blank group. Hematoxylin-eosin (HE) staining and lung wet/dry ratio measurement were used to evaluate the lung injury. The levels of inflammatory factors were measured by ELISA. High-throughput sequencing was used to measure the expression profile of each gene. Protein expression profiles were determined by label-free relative quantification of the differential proteome.ResultsLung injury such as the disordered structure of alveolar wall and inflammatory factors (IL-1β, IL-18, and IL-33) were significantly increased in the P-ALI group (p &lt; 0.05). There were 225 differentially expressed lncRNAs, including 85 upregulated and 140 downregulated genes. They were also the genomes with the most significant changes in transcriptome gene expression, mainly constituting cytoplasmic, synaptic structures and transporters, and involved in amino acid and carbon metabolism. There were 42 differentially expressed circRNAs, including 25 upregulated genes and 17 downregulated genes, mainly involved in cell composition, growth, differentiation, and division. There were only 10 differentially expressed miRNAs genes, all upregulated and mainly involved in the inflammatory response pathway. Proteome identification showed 79 differentially expressed proteins. KEGG enrichment analysis showed that it was mainly involved in the N-glycan biosynthesis pathway.ConclusionWe discovered that differentially regulated genes (lncRNAs, circRNAs, and miRNAs) were primarily associated with neuronal reflexes and synaptic signaling, including neurotransmitter transmission, ion signaling pathway conduction, neuronal projection, and synaptic vesicle circulation. They affected inflammatory factors and other metabolic pathways. This finding could be explored in future studies.
Single-cell analysis of bronchoalveolar cells in inflammatory and fibrotic post-COVID lung disease
Authors: Mehta Puja , Sanz-Magallón Duque de Estrada Blanca , Denneny Emma K. , Foster Kane , Turner Carolin T. , Mayer Andreas , Milighetti Martina , Platé Manuela , Worlock Kaylee B. , Yoshida Masahiro , Brown Jeremy S. , Nikolić Marko Z. , Chain Benjamin M. , Noursadeghi Mahdad , Chambers Rachel C. , Porter Joanna C. , Tomlinson Gillian S. 
Published: May 2024

BackgroundPersistent radiological lung abnormalities are evident in many survivors of acute coronavirus disease 2019 (COVID-19). Consolidation and ground glass opacities are interpreted to indicate subacute inflammation whereas reticulation is thought to reflect fibrosis. We sought to identify differences at molecular and cellular level, in the local immunopathology of post-COVID inflammation and fibrosis.MethodsWe compared single-cell transcriptomic profiles and T cell receptor (TCR) repertoires of bronchoalveolar cells obtained from convalescent individuals with each radiological pattern, targeting lung segments affected by the predominant abnormality.ResultsCD4 central memory T cells and CD8 effector memory T cells were significantly more abundant in those with inflammatory radiology. Clustering of similar TCRs from multiple donors was a striking feature of both phenotypes, consistent with tissue localised antigen-specific immune responses. There was no enrichment for known SARS-CoV-2-reactive TCRs, raising the possibility of T cell-mediated immunopathology driven by failure in immune self-tolerance.ConclusionsPost-COVID radiological inflammation and fibrosis show evidence of shared antigen-specific T cell responses, suggesting a role for therapies targeting T cells in limiting post-COVID lung damage.
Neuroserpin, IL-33 and IL-17A as potential markers of mild symptoms of depressive syndrome in Toxoplasma gondii-infected pregnant women
Authors: Silva Zolder Marinho , Toledo Débora Nonato Miranda , Pio Sirlaine , Machado Bianca Alves Almeida , Santos Priscilla Vilela dos , Hó Flávia Galvão , Medina Yasmim Nogueira , Cordeiro Paulo Henrique de Miranda , Perucci Luiza Oliveira , Pinto Kelerson Mauro de Castro , Talvani André 
Published: May 2024

IntroductionDepressive syndrome (DS) is a common complication during pregnancy and the postpartum period, and is triggered by multiple organic/genetic and environmental factors. Clinical and biochemical follow-up is essential for the early diagnosis and prognosis of DS. The protozoan Toxoplasma gondii causes infectious damage to the fetus during parasite primary-infection. However, in long-term infections, pregnant women develop immune protection to protect the fetus, although they remain susceptible to pathological or inflammatory effects induced by T. gondii. This study aimed to investigate plasma inflammatory biomarkers in pregnant women seropositive and seronegative for T. gondii, with diagnoses of minor and moderate/severe DS. MethodsPregnant women (n=45; age=18–39 years) were recruited during prenatal care at health centers in Ouro Preto, Minas Gerais, Brazil. Participants were asked to complete a socio-demographic questionnaire to be submitted to well-standardized DS scale calculators (Beck Depression Inventory Questionnaire, Edinburgh Postnatal Depression Scale, and Major Depressive Episode Module). Additionally, 4 mL of blood was collected for plasma neuroserpin, CCL2, IL-17A, and IL-33 analysis. ResultsPregnant volunteers with chronic T. gondii contact were all IgG+ (44%; n=21) and exhibited increased plasma IL-33, IL-17A, and neuroserpin levels, but not CCL2, compared to uninfected pregnant women. Using Beck’s depression inventory, we observed an increase in plasma IL-17A and IL-33 in women with T. gondii infeCction diagnosed with mild DS, whereas neuroserpin was associated with minor and moderate/severe DS. DiscussionOur data suggest a close relationship between DS in pregnant women with chronic T. gondii infection and neurological conditions, which may be partially mediated by plasma neuroserpin, IL-33, and IL-17A levels.
S1PR2 participates in intestinal injury in severe acute pancreatitis by regulating macrophage pyroptosis
Authors: Lin Tianjiao , Peng Mengyuan , Zhu Qingyun , Pan Xinting 
Published: May 2024

BackgroundSevere acute pancreatitis (SAP) is an inflammatory disorder affecting the gastrointestinal system. Intestinal injury plays an important role in the treatment of severe acute pancreatitis. In this study, we mainly investigated the role of S1PR2 in regulating macrophage pyroptosis in the intestinal injury of severe acute pancreatitis.MethodsThe SAP model was constructed using cerulein and lipopolysaccharide, and the expression of S1PR2 was inhibited by JTE-013 to detect the degree of pancreatitis and intestinal tissue damage in mice. Meanwhile, the level of pyroptosis-related protein was detected by western blot, the level of related mRNA was detected by PCR, and the level of serum inflammatory factors was detected by ELISA. In vitro experiments, LPS+ATP was used to construct the pyroptosis model of THP-1. After knockdown and overexpression of S1PR2, the pyroptosis proteins level was detected by western blot, the related mRNA level was detected by PCR, and the level of cell supernatant inflammatory factors were detected by ELISA. A rescue experiment was used to verify the sufficient necessity of the RhoA/ROCK pathway in S1PR2-induced pyroptosis. Meanwhile, THP-1 and FHC were co-cultured to verify that cytokines released by THP-1 after damage could regulate FHC damage.ResultsOur results demonstrated that JTE-013 effectively attenuated intestinal injury and inflammation in mice with SAP. Furthermore, we observed a significant reduction in the expression of pyroptosis-related proteins within the intestinal tissue of SAP mice upon treatment with JTE-013. We confirmed the involvement of S1PR2 in THP-1 cell pyroptosis in vitro. Specifically, activation of S1PR2 triggered pyroptosis in THP-1 cells through the RhoA/ROCK signaling pathway. Moreover, it was observed that inflammatory factors released during THP-1 cell pyroptosis exerted an impact on cohesin expression in FHC cells.ConclusionThe involvement of S1PR2 in SAP-induced intestinal mucosal injury may be attributed to its regulation of macrophage pyroptosis.
An inclusive approach to designing a multi-epitope chimeric vaccine for Taenia infections by integrating proteomics and reverse vaccinology
Authors: Sharma Swati , Sharan Ujjawal , Kaur Rimanpreet , Chaudhary Anubha , Rawat Suraj S. , Keshri Anand K. , Arora Naina , Prasad Amit 
Published: May 2024

BackgroundSoil- and water-transmitted helminths are a major concern in the developing world due to their high prevalence. More than a quarter of the population were estimated to be infected with helminths in these endemic zones.Research designAn in silico approach was used to design a vaccine construct against the Taenia genus utilizing the proteomic information and evaluation of the construct using immune-informatics.ResultsOur study identified 451 conserved proteins in Taenia spp. using the existing proteome; out of these, 141 were found to be expressed in cysticerci. These proteins were screened for antigenic epitopes and a multi-subunit vaccine was constructed. The constructed vaccine was assessed for its efficacy in mounting the appropriate immune response. Our constructed vaccine showed stability and optimal performance against the TLR 4 receptor, which is reported to be upregulated in Taenia infections in hosts.ConclusionImmune-informatics tools help design vaccines for neglected diseases such as those attributed to helminths, which are known to cause widespread morbidity. Our vaccine construct holds tremendous potential in conferring protection against all Taenia spp. of clinical relevance to human.