bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electronic and vibrational optical activity of several peptides related to neurohypophyseal hormones: Disulfide group conformation
Authors: Pazderková Markéta , Bednárová Lucie , Dlouhá Helena , Flegel Martin , Lebl Michal , Hlaváček Jan , Setnička Vladimír , Urbanová Marie , Hynie Sixtus , Klenerová Věra , Baumruk Vladimír , Maloň Petr 
Published: June 2012

AbstractElectronic and vibrational optical activity of the set of neurohypophyseal hormones and their analogs was investigated to clarify the SS bond solution conformation. The selected compounds include oxytocin (I), lysine vasopressin (II), arginine vasopressin (III), and their analogs (IV–IX), differing widely in their pharmacological properties. We have extended the already known electronic circular dichroism data by new information provided by vibrational circular dichroism (VCD) and Raman optical activity (ROA). The use of VCD brought additional details on three‐dimensional structure of the chain reversal in the ring moiety and on its left handedness. Furthermore, Raman scattering and ROA allowed us to deduce the sense of the disulfide bond torsion. © 2012 Wiley Periodicals, Inc. Biopolymers 97:923–932, 2012.
A novel technique for in situ aggregation of Gluconobacter oxydans using bio‐adhesive magnetic nanoparticles
Authors: Ni Kefeng , Lu Huimin , Wang Cunxun , Black Kvar C.L. , Wei Dongzhi , Ren Yuhong , Messersmith Phillip B. 
Published: June 2012

AbstractHere, we present a novel technique to immobilize magnetic particles onto whole Gluconobacter oxydans in situ via a synthetic adhesive biomimetic material inspired by the protein glues of marine mussels. Our approach involves simple coating of a cell adherent polydopamine film onto magnetic nanoparticles, followed by conjugation of the polydopamine‐coated nanoparticles to G. oxydans which resulted in cell aggregation. After optimization, 21.3 mg (wet cell weight) G. oxydans per milligram of nanoparticle was aggregated and separated with a magnet. Importantly, the G. oxydan aggregates showed high specific activity and good reusability. The facile approach offers the potential advantages of low cost, easy cell separation, low diffusion resistance, and high efficiency. Furthermore, the approach is a convenient platform technique for magnetization of cells in situ by direct mixing of nanoparticles with a cell suspension. Biotechnol. Bioeng. 2012; 109: 2970–2977. © 2012 Wiley Periodicals, Inc.
Detection of cellular response to titanium dioxide nanoparticle agglomerates by sensor cells using heat shock protein promoter
Authors: Chen Peng , Kanehira Koki , Sonezaki Shuji , Taniguchi Akiyoshi 
Published: June 2012

AbstractNanotechnology is becoming increasingly important for products used in our daily lives, such as the masses of titanium dioxide nanoparticle agglomerates (TiO2 NPs) used in the pharmaceutical industry, for cosmetic products, or for pigments. Meanwhile, a serious lack of detailed information concerning the interaction between the nanomaterials and cells limits their biological and medical applications. Sensing technology is very important for understanding these interactions. We have shown that TiO2 NPs induce heat shock protein 70B' (HSP70B') mRNA [Okuda‐Shimazaki et al., 2010. Int J Mol Sci 11:2383–2392]. In the current work, sensor cells for detection of cellular responses to NPs were prepared by transfecting an HSP70B' promoter–reporter plasmid. First, to find suitable cells for detection, five different mammalian cell lines were chosen as potential sensor cells. The results showed TiO2 NP response in some cell lines, although different sensor cells had different TiO2 NP response levels, as heat shock response ability is important for the detection. Then, we studied the TiO2 NP time‐course response and dose response. The results indicated that our sensor cells can detect TiO2 NP cellular responses. Our work should aid in understanding the interactions between bio‐nanomaterials and cells. Biotechnol. Bioeng. 2012; 109: 3112–3118. © 2012 Wiley Periodicals, Inc.
Design and validation of a corneal bioreactor
Authors: Leonard Elissa K. , Pai Vincent H. , Amberg Philip , Gardner Jens , Orwin Elizabeth J. 
Published: June 2012

AbstractMechanical strain is an important signal that influences the behavior and properties of cells in a wide variety of tissues. Physiologically similar mechanical strain can revert cultured cells to a more normal phenotype. Here, we have demonstrated that 3% equibiaxial (EB) and uniaxial strains confer favorable protein expression in cultured rabbit corneal fibroblasts (RCFs), with approximately 35% and 65% reduction in expression of α‐smooth muscle actin (α‐SMA), respectively. We have designed a novel bioreactor that is capable of imparting up to 7% EB strain and up to 6% EB strain using a cornea‐shaped post. Additional features of the bioreactor include the application of shear stress to cells in culture and the ability to image cells using optical coherence microscopy (OCM) without being removed from the system. Biotechnol. Bioeng. 2012; 109: 3189–3198. © 2012 Wiley Periodicals, Inc.
Development of chimeric laccases by directed evolution
Authors: Pardo Isabel , Vicente Ana Isabel , Mate Diana M. , Alcalde Miguel , Camarero Susana 
Published: June 2012

AbstractDNA recombination methods are useful tools to generate diversity in directed evolution protein engineering studies. We have designed an array of chimeric laccases with high‐redox potential by in vitro and in vivo DNA recombination of two fungal laccases (from Pycnoporus cinnabarinus and PM1 basidiomycete), which were previously tailored by laboratory evolution for functional expression in Saccharomyces cerevisiae. The laccase fusion genes (including the evolved α‐factor prepro‐leaders for secretion in yeast) were subjected to a round of family shuffling to construct chimeric libraries and the best laccase hybrids were identified in dual high‐throughput screening (HTS) assays. Using this approach, we identified chimeras with up to six crossover events in the whole sequence, and we obtained active hybrid laccases with combined characteristics in terms of pH activity and thermostability. Biotechnol. Bioeng. 2012; 109: 2978–2986. © 2012 Wiley Periodicals, Inc.
Comparison of 30-day, 90-day and in-hospital postoperative mortality for eight different cancer types
Authors: Damhuis R A M , Wijnhoven B P L , Plaisier P W , Kirkels W J , Kranse R , van Lanschot J J 
Published: June 2012

Abstract

Background
Various definitions are used to calculate postoperative mortality. As variation hampers comparability between reports, a study was performed to evaluate the impact of using different definitions for several types of cancer surgery.

Methods
Population-based data for the period 1997–2008 were retrieved from the Rotterdam Cancer Registry for resectional surgery of oesophageal, gastric, colonic, rectal, breast, lung, renal and bladder cancer. Postoperative deaths were tabulated as 30-day, in-hospital or 90-day mortality. Postdischarge deaths were defined as those occurring after discharge from hospital but within 30 days.

Results
This study included 40 474 patients. Thirty-day mortality rates were highest after gastric (8·8 per cent) and colonic (6·0 per cent) surgery, and lowest after breast (0·2 per cent) and renal (2·0 per cent) procedures. For most tumour types, the difference between 30-day and in-hospital rates was less than 1 per cent. For bladder and oesophageal cancer, however, the in-hospital mortality rate was considerably higher at 5·1 per cent (+1·3 per cent) and 7·3 per cent (+2·8 per cent) respectively. For gastric, colonic and lung cancer, 1·0 per cent of patients died after discharge. For gastric, lung and bladder cancer, more than 3 per cent of patients died between discharge and 90 days.

Conclusion
The 30-day definition is recommended as an international standard because it includes the great majority of surgery-related deaths and is not subject to discharge procedures. The 90-day definition, however, captures mortality from multiple causes; although this may be of less interest to surgeons, the data may be valuable when providing information to patients before surgery.
Surface‐based cryopreservation strategies for human embryonic stem cells: A comparative study
Authors: Malpique Rita , Tostões Rui , Beier Axel F. J. , Serra Margarida , Brito Catarina , Schulz Julia C. , Björquist Petter , Zimmermann Heiko , Alves Paula M. 
Published: June 2012

AbstractHuman embryonic stem cells (hESC) hold tremendous potential in the emerging fields of gene and cell therapy as well as in basic scientific research. One of the major challenges regarding their application is the development of efficient cryopreservation protocols for hESC since current methods present poor recovery rates and/or technical difficulties which impair the development of effective processes that can handle bulk quantities of pluripotent cells. The main focus of this work was to compare different strategies for the cryopreservation of adherent hESC colonies. Slow‐rate freezing protocols using intact hESC colonies was evaluated and compared with a surface‐based vitrification approach. Entrapment within ultra‐high viscous alginate was investigated as the main strategy to avoid the commonly observed loss of viability and colony fragmentation during slow‐rate freezing. Our results indicate that entrapment beneath a layer of ultra‐high viscous alginate does not provide further protection to hESC cryopreserved through slow‐rate freezing, irrespectively of the cryomedium used. Vitrification of adherent hESC colonies on culture dishes yielded significantly higher recovery rates when compared to the slow‐rate freezing approaches investigated. The pluripotency of hESC was not changed after a vitrification/thawing cycle and during further propagation in culture. In conclusion, from the cryopreservation methods investigated in this study, surface‐based vitrification of hESC has proven to be the most efficient for the cryopreservation of intact hESC colonies, reducing the time required to amplify frozen stocks thus supporting the widespread use of these cells in research and clinical applications. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 28: 1079–1087, 2012
Low dose tissue plasminogen activator treatment for vascular thrombosis following cardiac catheterization in children: A single center experience
Authors: Bratincsák András , Moore John W. , El‐Said Howaida G. 
Published: June 2012

ObjectivesTo assess the efficacy and safety of low dose tissue plasminogen activator (tPA) therapy in children with vascular thrombosis following cardiac catheterization.BackgroundCurrently, heparin is the standard of care for vascular thrombosis, however, it may not be efficacious in dissolving existing thrombi. Although tPA is an accepted thrombolytic therapy for life‐threatening thrombosis in children, it is not a well‐established treatment for the thrombosis of femoral vessels following cardiac catheterization.MethodsA retrospective analysis was performed from 1/1/2009 until 6/30/2011 at Rady Children's Hospital—San Diego (RCHSD) reviewing all cases with venous or arterial thrombosis at the vascular access site following cardiac catheterization, and a survey was conducted among the Congenital Cardiovascular Interventional Study Consortium members regarding their experience in tPA therapy.ResultsAt RCHSD, out of 1,155 catheterizations, 12 children developed femoral thrombosis. In three children, where 12‐h heparin therapy was unsuccessful in resolving thrombosis, we used low‐dose tPA according to the following regime: 0.05 mg/kg/h for 30 min, followed by 0.1 mg/kg/h for 4 h. We achieved thrombolysis in all cases evidenced by repeat ultrasound and return of palpable pulse with normal limb circulation. No complications were encountered. The survey confirmed that adult tPA dose (0.1–0.5 mg/kg/h) has 100% efficacy in resolving thrombi in children with vascular thrombosis, however, the rate of serious complications was not negligible (15%).ConclusiontPA is an efficacious therapy to dissolve thrombi that developed as a complication of cardiac catheterization in children. The rate of complications due to tPA may be reduced using a lower dose: 0.05–0.1 mg/kg/h. © 2012 Wiley Periodicals, Inc.
Reduction of the rotational ambiguity of curve resolution techniques under partial knowledge of the factors. Complementarity and coupling theorems
Authors: Sawall Mathias , Fischer Christian , Heller Detlef , Neymeyr Klaus 
Published: June 2012

Multivariate curve resolution techniques allow to uncover from a series of spectra (of a chemical reaction system) the underlying spectra of the pure components and the associated concentration profiles along the time axis. Usually, a range of feasible solutions exists because of the so‐called rotational ambiguity. Any additional information on the system should be utilized to reduce this ambiguity.Sometimes the pure component spectra of certain reactants or products are known, or the concentration profiles of the same or other species are available. This valuable information should be used in the computational procedure of a multivariate curve resolution technique.The aim of this paper is to show how such supplemental information on the components can be exploited. The knowledge of spectra leads to linear restrictions on the concentration profiles of the complementary species and vice versa. Further, affine–linear restrictions can be applied to pairs of a concentration profile and the associated spectrum of a species. These (affine) linear constraints can also be combined with the usual non‐negativity restrictions. These arguments can reduce the rotational ambiguity considerably. In special cases, it is possible to determine the unknown concentration profile or the spectrum of a species only from these constraints. Copyright © 2012 John Wiley & Sons, Ltd.
The Measurement of Lipids Currently and 9 Years Ago—Which Is More Associated With Carotid Intima‐Media Thickness?
Authors: Huang Yubei , Yu Xuequn , Millican Danielle , Wu Liuxin , Shi Ping , Lu Min , Wu Yangfeng 
Published: June 2012

AbstractBackground:Massive evidence supports that increase of lipids bring more risk of atherosclerosis. However, it is not clear if lipids measured a long time ago bear more risk than the current measurement.Hypothesis:Lipids measured currently is more associated with carotid atherosclerosis than lipids measured long time ago.Methods:A cohort of 1195 participants age 35 to 64 years was examined in both 1993–1994 and 2002 for serum lipids, and in 2002 for carotid intima‐media thickness (CIMT) with B mode ultrasound. The associations of lipids at baseline and at reexamination with CIMT were analyzed and compared using multiple linear regressions.Results:All lipid variables, except for high‐density lipoprotein cholesterol (HDL‐C) both at baseline and reexamination, were significantly associated with age‐adjusted CIMT in both males and females (all Ptrend <0.01). The age‐adjusted mean of CIMT in all of the population was 0.696 mm in those having low low‐density lipoprotein cholesterol (LDL‐C) at both examinations, 0.719 mm in those having high LDL‐C only at baseline, 0.706 mm in those having high LDL‐C only at reexamination, and 0.727 mm in those having high LDL‐C at both examinations. Further analysis showed that lipids measured at baseline remained significant, whereas lipids at reexamination became not significant in all models, except those for HDL‐C and total cholesterol (TC)/HDL‐C, which allow the lipids at different times to compete in association with CIMT.Conclusions:Both the current measurement of lipids (TC, LDL‐C, non‐HDL‐C, TC/HDL‐C, and LDL‐C/HDL‐C) and the measurement from 9 years ago are significantly associated with CIMT, but the measurement from 9 years ago had an even stronger association. Clin. Cardiol. 2012 doi: 10.1002/clc.22015Additional Supporting Information may be found in the online version of this article.The authors have no funding, financial relationships, or conflicts of interest to disclose.
Reuse of Distillery Wastewater with Designed Dose and Pattern for Sugarcane Irrigation
Authors: Chauhan Jaspal S. , Rai Jai Prakash Narayan 
Published: June 2012

AbstractThe present investigation aims to optimize dose and pattern of distillery effluent for sugarcane irrigation. The postmethanated distillery effluent (PMDE) was recorded to have significant amount of micro‐ (Na, Zn, Fe) and macro‐ (Ca, Mg, N‐NO3, P, K, S–SO) nutrients and so was utilized for sugarcane irrigation. Lysimetric studies were conducted to assess the impact of PMDE on sugarcane productivity with different concentrations (50 and 75%) and irrigation patterns (intermittent and pre‐sowing). The intermittent pattern of ferti‐irrigation with 50 and 75% effluent dose for sugarcane crop was found to enhance the growth and quality parameters of crop without impairing the groundwater quality. Results were more pronounced with 75% intermittent irrigation as the percent increase with respect to control for plant length, cane girth, cane weight, number of internodes per cane, dry matter accumulation, juice extraction, sucrose content, and available sugar were 28.0, 42.5, 14.6, 40.2, 54.4, 18.9, 44.9, 57.9, and 50.0%, respectively. It is suggested that PMDE can be used as an alternative of fresh water irrigation and also as a fertilizer for sugarcane, provided that the effluent quality and sugarcane quality is continuously monitored to avoid any contamination.
Recovery of Polyvinyl Alcohol from Desizing Wastewater Using a Novel High‐Shear Ultrafiltration Module
Authors: Sarkar Ankur , Sarkar Debasish , Gupta Madhurima , Bhattacharjee Chiranjib 
Published: June 2012

AbstractPolyvinyl alcohol (PVA), the major constituent of desizing water constituting 45% of the total BOD load has a significant environmental impact owing to its poor biodegradability. In order to prevent PVA from being discharged by the effluent stream, modern textile industries opt for membrane based separation techniques using ultrafiltration so that the recovery and recycle of PVA in tandem could be achieved. However, the process of ultrafiltration is still not widely accepted as expected due to the well‐known non‐idealities of concentration polarization and pore blockage. In this article, design and performance characterization of a lab‐scale novel shear enhanced ultrafiltration unit, named as spinning basket membrane (SBM) module are discussed. The proposed module is unique in terms of its inbuilt cleaning facility eliminating the effects of polarization and subsequent periodic fouling leading to its uninterrupted production operation. The test fluid, necessarily a solution of PVA was treated in the proposed module under different parametric conditions with polyvinylidene fluoride (PVDF) membranes of two different molecular weight cut‐off (50 and 100 kDa). After 2 h of continuous operation the permeate flux was observed to be within 95–97% of the respective initial fluxes. Such performance is rarely been attained in practice. Hence, the novelty of the present research is achieved. Considering the performance of the present module in terms of flux regeneration and product recovery, it may be regarded as an efficient device and can be potentially deployed for cleaning of other industrial wastewater.
Effects of Cationic Structure on Cellulose Dissolution in Ionic Liquids: A Molecular Dynamics Study
Authors: Zhao Yuling , Liu Xiaomin , Wang Jianji , Zhang Suojiang 
Published: June 2012

AbstractIn recent years, great progress has been made in the dissolution of cellulose with ionic liquids (ILs). However, the mechanism of cellulose dissolution, especially the role the IL cation played in the dissolution process, has not been clearly understood. Herein, the mixtures of cellulose with a series of imidazolium‐based chloride ionic liquids and 1‐butyl‐3‐methyl pyridinium chloride ([C4mpy]Cl) were simulated to study the effect that varying the heterocyclic structure and alkyl chain length of the IL cation has on the dissolution of cellulose. It was shown that the dissolution of cellulose in [C4mpy]Cl is better than that in [C4mim]Cl. For imidazolium‐based ILs, the shorter the alkyl chain is, the higher the solubility will be. In addition, an all‐atom force field for 1‐allyl‐3‐methyl imidazolium cation ([Amim]+) was developed, for the first time, to investigate the effect the electron‐withdrawing group within the alkyl chain of the IL cation has on the dissolution of cellulose. It was found that the interaction energy between [Amim]+ and cellulose was greater than that between [C3mim]+ and cellulose, indicating that the presence of electron‐withdrawing group in alkyl chain of the cation enhanced the interaction between the cation and cellulose due to the increase of electronegativity of the cations. These findings are used to assess the cationic effect on the dissolution of cellulose in ILs. They are also expected to be important for rational design of novel ILs for efficient dissolution of cellulose.
Adaptive scheduling mechanism for IPTV over WiMAX IEEE 802.16j networks
Authors: Brahmia Mohamed‐el‐Amine , Abouaissa Abdelhafid , Lorenz Pascal 
Published: June 2012

SUMMARYA WiMAX technology is a very promising Broadband Wireless Access technology that is able to transmit different service types. This latter can have different constraints such as traffic rate, maximum latency, and tolerated jitter. The IEEE 802.16 Medium Access Control specifies five types of QoS classes: UGS, rtPS, ertPS, nrtPS, and BE. However, the IEEE 802.16 standard does not specify the scheduling algorithm to be used. Operators have the choice among many existing scheduling techniques. Also, they can propose their own scheduling algorithms. In this paper, we propose a scheduling strategy (Adaptive Weighted Round Robin, AWRR) for various Internet Protocol Television (IPTV) services traffic over 802.16j networks. Our scheme adapts dynamically the scheduler operation to according queue load and quality of service constraints. In particular, the proposed mechanism gives more priority to high definition television and standard definition television traffic by using two schedulers. The proposed scheduling algorithm has been simulated using the QualNet network simulator. The experimental results show that our scheduler schemes AWRR have a better performance than the traditional scheduling techniques for rtPS traffic, which allows ensuring QoS requirements for IPTV application. Copyright © 2012 John Wiley & Sons, Ltd.
Eating disorder symptoms and weight and shape concerns in a large web‐based convenience sample of women ages 50 and above: Results of the gender and body image (GABI) study
Authors: Gagne Danielle A. , Von Holle Ann , Brownley Kimberly A. , Runfola Cristin D. , Hofmeier Sara , Branch Kateland E. , Bulik Cynthia M. 
Published: June 2012

AbstractObjective:Limited research exists on eating disorder symptoms and attitudes and weight and shape concerns in women in midlife to older adulthood. We conducted an online survey to characterize these behaviors and concerns in women ages 50 and above.Method:Participants (n = 1,849) were recruited via the Internet and convenience sampling.Results:Eating disorder symptoms, dieting and body checking behaviors, and weight and shape concerns were widely endorsed. Younger age and higher body mass index (BMI) were associated with greater endorsement of eating disorder symptoms, behaviors, and concerns.Discussion:Weight and shape concerns and disordered eating behaviors occur in women over 50 and vary by age and BMI. Focused research on disordered eating patterns in this age group is necessary to develop age‐appropriate interventions and to meet the developmental needs of an important, growing, and underserved population. © 2012 by Wiley Periodicals, Inc. (Int J Eat Disord 2012;45:832–844)