bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diet of the twaite shad Alosa fallax (Lacépède, 1803) (Clupeidae) in the Gulf of Gdansk, the Baltic Sea
Authors: Skóra Michał , Sapota Mariusz , Skóra Krzysztof , Pawelec Anna 
Published: June 2012

Abstract
Twaite shad Alosa fallax (12–48 cm total length) from the Gulf of Gdansk feed on Mysids: Mysis mixta, Neomysis integer, and fish: Sprattus sprattus, Osmerus eperlanus, and Pomatoschistus sp.. In the stomachs of A. fallax there also occur Hydrobia sp., Diptera and small stones, particles of wood and plastic. The food of inshore twaite shad measured from 12 to 21 cm centered on M. mixta. Fish are the main prey for A. fallax from the class length 22–31 cm from the Vistula River mouth and for all shads longer than 32 cm.
Tentations, impulsions et contrôle des consommateurs sur les sites de ventes privées
Authors: Ayadi Nawel , Giraud Magali , Gonzalez Christine 
Published: June 2012

Résumé Les ventes privées sur Internet existent et connaissent un fort succès depuis une dizaine d’années. Elles consistent à mettre en vente sur un site web, pour une durée limitée à quelques jours, des stocks limités de produits de grandes marques, à des prix bradés, auxquels ont accès exclusivement les clients membres du site. Ces stratégies visent à provoquer des achats impulsifs en jouant sur l’impression de faire une bonne affaire. Cette recherche se pose deux questions. D’une part, comment les sites de ventes privées poussent-ils leurs visiteurs à réaliser des achats impulsifs ? D’autre part, est-ce que les consommateurs développent des stratégies de contrôle pour mieux maîtriser leurs achats ? La littérature sur les bonnes affaires et l’achat impulsif a été mobilisée pour construire le cadre théorique de cette étude. Une étude qualitative a ensuite été réalisée auprès de trente-quatre membres de ces sites.
Le dispositif de gestion des organisations hybrides, régulateur de logiques institutionnelles hétérogènes ? Le cas du capital-risque solidaire
Authors: Château Terrisse Pascale 
Published: June 2012

Résumé Cet article s’intéresse aux micro-fondations du néo-institutionnalisme, en analysant les logiques institutionnelles traduites dans le dispositif de gestion d’une organisation de capital-risque solidaire. Cette analyse est issue d’une recherche intervention et de l’analyse de contenu d’entretiens. Il tentera de répondre aux questions suivantes : Comment les logiques institutionnelles de la finance solidaire se manifestent dans le dispositif de gestion des organisations ? Quels rôles jouent le dispositif de gestion dans la gestion des tensions entre les logiques ? Ces logiques institutionnelles de la finance solidaire sont d’un côté la logique financière et de l’autre côté la logique solidaire. Ces logiques sont contradictoires et sources de tensions et de conflits. Le dispositif de gestion de l’organisation reflète les logiques dans sa structure et permet d’équilibrer les logiques. Pour hybrider les logiques, le pouvoir des acteurs qui forment des coalitions distinctes supportant chacune une logique, doit être réparti. Les outils du dispositif doivent d’une part avoir un substrat technique reflétant l’ensemble des logiques et être assez flexible pour susciter l’intéressement de l’ensemble des acteurs, et d’autre part être dotés d’une philosophie managériale et d’une vision simplifiée des acteurs cohérentes qui poussent à la communication et à la traduction des logiques.
Virologically Suppressed HIV Patients Show Activation of NK Cells and Persistent Innate Immune Activation
Authors: Lichtfuss Gregor F. , Cheng Wan-Jung , Farsakoglu Yagmur , Paukovics Geza , Rajasuriar Reena , Velayudham Pushparaj , Kramski Marit , Hearps Anna C. , Cameron Paul U. , Lewin Sharon R. , Crowe Suzanne M. , Jaworowski Anthony 
Published: June 2012

Abstract
FcRγ is an ITAM-containing adaptor required for CD16 signaling and function in NK cells. We have previously shown that NK cells from HIV patients receiving combination antiretroviral therapy (cART) have decreased FcRγ expression, but the factors causing this are unknown. We conducted a cross-sectional study of cART-naive viremic patients (ART−), virologically suppressed patients receiving cART (ART+), and HIV-uninfected controls. CD8+ T cells were activated, as assessed by CD38+HLA-DR+ expression, in ART− patients (p &lt; 0.0001), which was significantly reduced in ART+ patients (p = 0.0005). In contrast, CD38+HLA-DR+ NK cells were elevated in ART− patients (p = 0.0001) but did not decrease in ART+ patients (p = 0.88). NK cells from both ART− and ART+ patients showed high levels of spontaneous degranulation in ex vivo whole blood assays as well as decreased CD16 expression (p = 0.0001 and p = 0.0025, respectively), FcRγ mRNA (p &lt; 0.0001 for both groups), FcRγ protein expression (p = 0.0016 and p &lt; 0.0001, respectively), and CD16-dependent Syk phosphorylation (p = 0.0001 and p = 0.003, respectively). HIV-infected subjects showed alterations in NK activation, degranulation, CD16 expression and signaling, and elevated plasma markers of inflammation and macrophage activation, that is, neopterin and sCD14, which remained elevated in ART+ patients. Alterations in NK cell measures did not correlate with viral load or CD4 counts. These data show that in HIV patients who achieve viral suppression following cART, NK cell activation persists. This suggests that NK cells respond to factors different from those driving T cell activation, but which are associated with inflammation in HIV patients.
Patient Handling Methods Taught in Occupational Therapy Curricula
Authors: Frost Lenore , Barkley William M. 
Published: June 2012

Abstract
Sixteen of 100 full-time occupational therapists are injured while performing manual patient handling techniques. We developed a Theory of Planned Behavior self-report questionnaire to determine what educators teach and the behavioral constructs that best predict intention to change curriculum content. Traditional manual patient handling and safe patient handling methods were investigated. The results showed that both methods are taught in most programs; however, only 22% stated that they teach safe patient handling as the standard of practice. Stepwise regression analysis demonstrated that attitude and perceived behavioral control are the best predictors of intention to continue teaching manual transfers as the standard; however, normative belief and attitude best predict intention to teach safe patient handling as the standard. Knowing these predictors will assist in developing strategies to promote a paradigm shift in the way patient transfers are taught.
Comparison of Reverse Transcription-Polymerase Chain Reaction, Immunohistochemistry, and Fluorescence In Situ Hybridization Methodologies for Detection of Echinoderm Microtubule-Associated Proteinlike 4–Anaplastic Lymphoma Kinase Fusion–Positive Non–Small Cell Lung Carcinoma: Implications for Optimal Clinical Testing
Authors: Wallander Michelle L. , Geiersbach Katherine B. , Tripp Sheryl R. , Layfield Lester J. 
Published: June 2012

Context.—Echinoderm microtubule–associated proteinlike 4–anaplastic lymphoma kinase (EML4-ALK) gene fusions are detected in 3% to 13% of non–small cell lung carcinomas. Accurate testing for detection of EML4-ALK fusions is essential for appropriate therapy selection.
Objective.—To compare reverse transcription-polymerase chain reaction (RT-PCR), immunohistochemistry (IHC), and fluorescence in situ hybridization (FISH) methodologies for detection of EML4-ALK fusions.
Design.—Forty-six pulmonary adenocarcinomas were selected with enrichment for wild-type epidermal growth factor receptor (EGFR) status (wild type, n  =  42; mutant, n  =  4). Specimens were tested by IHC (Dako; clone ALK1), FISH (Abbott Molecular; LSI ALK break apart), and RT-PCR (variants 1 and 3a/b).
Results.—EML4-ALK variant 3a/b was detectable by RT-PCR, FISH, and IHC in 4% (2 of 46) of specimens. Complete agreement among FISH and IHC reviewers was obtained for variant 3a/b. No concordance existed among methodologies for the detection of EML4-ALK variant 1. The RT-PCR method detected variant 1 in 20% (9 of 46) of specimens. Agreement among FISH viewers was poor for variant 1 because only 11% (1/9) of specimens were scored as positive by all 3 viewers. The sensitivity of IHC for detection of variant 1 was also poor because only 1 of 9 samples (11%) was scored as positive. Overall, the frequency of EML4-ALK variants 1 and 3a/b was 24% (11 of 46) in adenocarcinomas enriched for wild-type EGFR status. One EML4-ALK variant 1 fusion was found to coexist with an EGFR exon 21 mutation.
Conclusions.—The FISH interpretation demonstrated great variability among observers. The RT-PCR method was the most sensitive and least-subjective methodology for detection of EML4-ALK fusions.
Biliary Atresia: A Multidisciplinary Approach to Diagnosis and Management
Authors: Moreira Roger Klein , Cabral Rodrigo , Cowles Robert A. , Lobritto Steven J. 
Published: June 2012

Context.—Biliary atresia is an inflammatory cholangiopathy of infancy that results in progressive fibrosis and obliteration of bile ducts and represents the main indication for liver transplant in young children. In spite of extensive investigation, its etiology has remained poorly understood. Timely surgical intervention (Kasai procedure) may result in significant benefit to these patients and represents the final goal of an accurate diagnostic evaluation.
Objective.—To present an overview of biliary atresia, including clinical and surgical approaches to this disease, with emphasis on the histopathologic evaluation.
Data Sources.—Review of relevant literature indexed in PubMed (US National Library of Medicine).
Conclusion.—A well-coordinated multidisciplinary approach is required in the assessment of suspected cases of biliary atresia. Pathologic examination of biopsy specimens is an integral part of the diagnostic algorithm and, therefore, plays a pivotal role in the diagnostic evaluation of this disease.
Effects of hyperbaric oxygen on motor function in children with cerebral palsy
Authors: Lacey Daniel J. , Stolfi Adrienne , Pilati Louis E. 
Published: June 2012

AbstractObjective:We conducted a randomized, double‐blind, controlled clinical trial to determine whether hyperbaric oxygen (HBO) improves gross motor function in children with cerebral palsy.Methods:Forty‐nine children aged 3 to 8 years with spastic cerebral palsy were randomized to 40 treatments of HBO (100% oxygen at 1.5atm) or hyperbaric air (HBA, 14% oxygen at 1.5atm) over an 8‐week period. The primary outcome was the Gross Motor Function Measure (GMFM) global score. Other outcomes included the Pediatric Evaluation of Disability Inventory (PEDI). Assessments were made before and immediately, 3 months, and 6 months after the treatment period. Within‐group changes were analyzed with paired t tests or repeated measures analysis of variance. Analysis of covariance was used for between‐group comparisons.Results:Forty‐six children (24 HBO, 22 HBA) were analyzed at the second interim analysis, which was scheduled to take place when at least half of the required number of patients in each group had completed pre‐ and post‐treatment testing. No changes occurred in the GMFM from pre‐ to post‐treatment in either group or between groups. Statistically significant increases occurred in both groups on the PEDI, with no difference between groups. The study was stopped because the calculated conditional probability of obtaining a difference between groups if the study continued to the end was only between 0.5% and 1.6%.Interpretation:HBO was not effective in improving GMFM scores, and was no more effective than HBA in improving PEDI scores. These results do not support use of HBO as a therapy for cerebral palsy in young children who did not have neonatal hypoxic–ischemic encephalopathy. ANN NEUROL 2012;72:695–703
Structure and Function of Noncanonical Nucleobases
Authors: Carell Thomas , Brandmayr Caterina , Hienzsch Antje , Müller Markus , Pearson David , Reiter Veronika , Thoma Ines , Thumbs Peter , Wagner Mirko 
Published: June 2012

AbstractDNA and RNA contain, next to the four canonical nucleobases, a number of modified nucleosides that extend their chemical information content. RNA is particularly rich in modifications, which is obviously an adaptation to their highly complex and variable functions. In fact, the modified nucleosides and their chemical structures establish a second layer of information which is of central importance to the function of the RNA molecules. Also the chemical diversity of DNA is greater than originally thought. Next to the four canonical bases, the DNA of higher organisms contains a total of four epigenetic bases: m5dC, hm5dC, f5dC und ca5dC. While all cells of an organism contain the same genetic material, their vastly different function and properties inside complex higher organisms require the controlled silencing and activation of cell‐type specific genes. The regulation of the underlying silencing and activation process requires an additional layer of epigenetic information, which is clearly linked to increased chemical diversity. This diversity is provided by the modified non‐canonical nucleosides in both DNA and RNA.
Synthesis of amphiphilic heteroarm star‐shaped polymer via free radical polymerization using polyfunctional chain transfer agent and its self‐assembly behavior
Authors: Lv Meng‐Qing , Shi Yan , Yang Wan‐Tai , Fu Zhi‐Feng 
Published: June 2012

AbstractAmphiphilic heteroarm star‐shaped polymers have important theoretical and practical significance. In this work, amphiphilic heteroarm star‐shaped polymer was synthesized by the use of polyfunctional chain transfer agent via sequential free radical polymerization in two steps. First, conventional free radical polymerization of methyl methacrylate (MMA) initiated by 2,2′‐azobis (isobutyronitrile) (AIBN) was carried out in the presence of polyfunctional chain transfer agent, pentaerythritol‐tertrakis (3‐mercaptopropinate) (PETMP). At appropriate monomer conversion, about two‐arm s‐PMMA having two residual thiol groups at the chain center was obtained. Second, the s‐PMMA obtained above was used as macro‐chain‐transfer agent for free radical polymerization of acrylic acid (AA). The heteroarm star‐shaped polymer with the hydrophobic PMMA segment and the hydrophilic PAA segment was obtained. The successful synthesis of heteroarm star‐shaped polymers, (PMMA)2(AA)2, was confirmed by 1H‐NMR and its self‐assembly behavior in different solvents. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
In situ Raman spectroscopy for simultaneous monitoring of multiple process parameters in mammalian cell culture bioreactors
Authors: Whelan Jessica , Craven Stephen , Glennon Brian 
Published: June 2012

AbstractIn this study, the application of Raman spectroscopy to the simultaneous quantitative determination of glucose, glutamine, lactate, ammonia, glutamate, total cell density (TCD), and viable cell density (VCD) in a CHO fed‐batch process was demonstrated in situ in 3 L and 15 L bioreactors. Spectral preprocessing and partial least squares (PLS) regression were used to correlate spectral data with off‐line reference data. Separate PLS calibration models were developed for each analyte at the 3 L laboratory bioreactor scale before assessing its transferability to the same bioprocess conducted at the 15 L pilot scale. PLS calibration models were successfully developed for all analytes bar VCD and transferred to the 15 L scale. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012
The society for cardiovascular angiography and interventions structural heart disease early career task force survey results: Endorsed by the society for cardiovascular angiography and interventions
Authors: Marmagkiolis Konstantinos , Hakeem Abdul , Cilingiroglu Mehmet , Bailey Steven R. , Ruiz Carlos , Hijazi Ziyad M. , Herrmann Howard C. , Zajarias Alan , Goldberg Steven L. , Feldman Ted 
Published: June 2012

AbstractOver the last decade, structural heart disease interventions have emerged as a new field in interventional cardiology. Currently, the Accreditation Council for Graduate Medical Education accredited interventional cardiology fellowship programs in the United States provide high‐quality and well established training curriculum in coronary and peripheral interventions, but training in structural interventions remains in its infancy. The current survey seeks to collect relevant information and assess the opinion of interventional cardiology program directors in ACGME‐accredited institutions that are actively involved in structural interventional training. Our study describes the actual number of structural procedures performed by interventional cardiology fellows in ACGME‐accredited programs, the form of the structural training today and the suggestions from program directors who are actively trying to integrate structural training in the interventional cardiology fellowship programs. © 2012 Wiley Periodicals, Inc.
Demasking large dummy effects approach in revealing important interactions in Plackett–Burman experimental design
Authors: Rakić Tijana , Jančić‐Stojanović Biljana , Malenović Andjelija , Ivanović Darko , Medenica Mirjana 
Published: June 2012

This paper presents a newly developed demasking large dummy effects (DDE) approach for identification of significant factor interactions in Plackett–Burman experimental design. Dummy factors, as imaginary factors, are not expected to contribute to the model, so the appearance of their high effect values could indicate the significance of the interactions they are confounded with. DDE approach consists of the screening of the large estimable effects, the calculation of the interactions that contribute the most to the large dummy effects from the alias matrix, and a single pass of all subsets regressions including preselected interactions and main factors for the final model tuning.Tested on experimentally obtained data from a robustness analysis of a method for the determination of raloxifene and its four impurities, DDE approach showed to create the models that have greater statistical power than the ones described with the main factors only, which makes them more reliable and accurate for the application. Additionally, because of the interactions selection criterion and final tuning phase of the strategy, DDE approach allows the creation of models that are likely to better describe the real systems, which increases the practical value. Copyright © 2012 John Wiley & Sons, Ltd.
Synthesis and Structural Characterization of Novel Camphor‐derived Amines
Authors: Grošelj Uroš , Sevšek Alen , Ričko Sebastijan , Golobič Amalija , Svete Jurij , Stanovnik Branko 
Published: June 2012

ABSTRACTTwo novel 4‐substituted camphidine derivatives 10a,b have been prepared from (+)‐camphor (1) in five steps, the Beckmann rearrangement being the bottleneck of the synthesis. Isoborneol derivative 5b, formed as a side product during the hydrogenation of arylidene ketone 3b, under Beckmann rearrangement conditions yielded interesting novel rearrangement products 11 and 12. (1S)‐(+)‐Camphorquinone (13) was transformed into diamines 15 and 16 in two steps, the former being cyclized into an imidazoline salt 17, an N‐heterocyclic carbene precursor. The structures of all novel compounds have been meticulously characterized using NMR techniques and/or single crystal X‐ray analysis. Chirality 24:778–788, 2012. © 2012 Wiley Periodicals, Inc.
Electrocardiographic QRS Duration Reflects Right Ventricular Remodeling in Patients Undergoing Corrective Surgery for Isolated Tricuspid Regurgitation: A Comparative Study with Cardiac Magnetic Resonance Imaging
Authors: Seo Myung‐Ki , Park Eun‐Ah , Kim Hyung‐Kwan , Lee Whal , Kim Yong‐Jin , Kim Kyung‐Hwan , Kim Ki‐Bong , Sohn Dae‐Won , Ahn Hyuk , Park Jae‐Hyung 
Published: June 2012

AbstractBackground:The role of electrocardiogram (ECG) is unclear for the longitudinal follow‐up of patients who undergo corrective surgery for isolated severe tricuspid regurgitation (TR).Hypothesis:This study sought to investigate the usefulness of changes in QRS duration of ECG after TR surgery in predicting right ventricular (RV) reverse remodeling as determined by cardiac magnetic resonance imaging (CMR).Methods:We enrolled 30 consecutive TR patients (27 women, aged 57.8 ± 9.6 years) who had undergone prior left‐sided valve surgery. A computer‐assisted analysis was performed for objective calculation of QRS duration before and after surgery.Results:At a median CMR follow‐up of 27.5 months postsurgery, QRS duration was cut by 14.6%, from 110.4 ± 14.6 msec to 96.9 ± 11.9 msec (P < 0.001), while CMR showed a decrease in RV end‐diastolic volume index (RV‐EDVI) from 179.5 ± 59.7 to 119.1 ± 30.4 mL/m2 (P < 0.001). QRS duration correlated significantly with RV‐EDVI and RV end‐systolic volume index (r = 0.65, P < 0.001 and r = 0.53, P < 0.001, respectively), and a percent change in QRS duration was significantly correlated with a percent change in RV‐EDVI (r = 0.40, P = 0.03). When significant RV reverse remodeling was defined as a reduction in RV‐EDVI ≥20% following TR surgery, the sensitivity and specificity for significant RV reverse remodeling were 75% and 78%, respectively, with a 9% reduction in QRS duration (P = 0.01, area underneath the receiver operator curve [AUC] = 0.81).Conclusions:The extent of changes in postoperative QRS duration can be used as a useful, inexpensive, and simple index reflecting the occurrence of significant RV reverse remodeling in patients undergoing corrective TR surgery. Clin. Cardiol. 2012 doi: 10.1002/clc.22030First two authors equally contributed to this work.This study was supported in part by grants from the Korea Health 21 R&D Project, Ministry of Health and Welfare, Republic of Korea (A090064) and Leading Foreign Research Institute Recruitment Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (MEST) (0640‐20100001). The authors have no funding, financial relationships, or conflicts of interest to disclose.