bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reduced bone mineral density in patients with haemophilia A and B in Northern Greece
Authors: Vakalopoulou Sofia , Slavakis Aristidis , Charizopoulou Maria , Kazantzidou Eirini , Chrysopoulou Tania , Vyzantiadis Timoleon-Achilleas , Moka Eleni , Agapidou Alexandra , Garipidou Vassilia , Anagnostis Panagiotis 
Published: February 2012

SummaryHaemophilia A and B has been associated with increased prevalence of low bone mass (67–86%). The aim of this study was to estimate the prevalence of bone disease in haemophiliacs and its association with potential risk factors. Adult patients with haemophilia A and B followed-up in the Haemophilia Centre of Northern Greece were included. Bone mineral density (BMD) was measured by dual-energy X-ray absorptiometry (DXA) in lumbar spine (LS), femoral neck (FN), total hip (TH) and great trochanter (GT). One-hundred four male patients (aged 45.8 ± 15.1 years) and 50 controls (aged 44.9 ± 12.8 years) were screened. Low BMD was diagnosed in 28 patients (26.9%) and 10 controls (20%) (p=0.0001). Patients had lower BMD in TH (p=0.007), FN (p=0.029) and GT (p=0.008) than controls, without differences in LS. BMD was positively associated with the severity of haemophilia, history of herpes virus C or human immunodeficiency virus and level of physical activity, and negatively with the level of arthropathy. In multiple-regression analysis, only the level of physical activity and 25-hydroxyvitamin D [25(OH)D] significantly predicted BMD. Half of the patients had vitamin D deficiency. In conclusion, our study showed increased prevalence of low BMD in haemophiliacs. The levels of physical activity and 25(OH)D independently predicted low BMD.
Prediction of thrombus resolution after intravenous thrombolysis assessed by CT-based thrombus imaging
Authors: Nam Hyo , Kim Eung , Kim Seo , Kim Young , Kim Jinkwon , Lee Hye , Nam Chung , Heo Ji 
Published: February 2012

SummaryThe degree of thrombus resolution directly indicates the effectiveness of a thrombolytic drug. We investigated the degree of thrombus resolution and factors associated with thrombus resolution after intravenous (IV) recombinant tissue plasminogen activator (rt-PA) using thin-section noncontrast computed tomography (NCT). Thin-section NCTs were performed before and immediately after IV rt-PA infusion in acute stroke patients. The thrombus volume and Hounsfield unit were measured using three-dimensional imaging software. Immediate recanalisation was assessed immediately after IV rt-PA infusion using CT angiography. During a three-year study period, 130 patients were prospectively enrolled. On baseline thin-section NCT, no thrombi were found in 30 patients (23%). Among the 100 patients with confirmed thrombus, the median volume decreased by 20% on the follow-up NCT. The thrombus was completely resolved in 8%. Of note, an increase in thrombus volume was observed in 20 patients. Independent predictors of thrombus resolution were total rt-PA dose, thrombus location in the M2 segment of the middle cerebral artery, and time from baseline to follow-up NCT. Thrombus resolution increased by 9% per each 10-mg increase in rt-PA (p = 0.045). Immediate complete recanalisation was achieved in 12% of patients. Total dose of rt-PA was independently associated with complete recanalisation [odds ratio [OR] 4.52, 95% confidence interval [CI] 1.345–15.184) and good functional outcome at three months (modified Rankin scale score <3, OR 2.34, 95% CI 1.104–4.962). In conclusion, rt-PA dose was associated with the degree of thrombus resolution, immediate complete recanalisation, and good outcome at three months. CT-based thrombus imaging may be helpful in determining thrombolysis effectiveness.
Herpesviruses enhance fibrin clot lysis
Authors: Gershom Edwin S. , Vanden Hoek Amanda L. , Meixner Scott C. , Sutherland Michael R. , Pryzdial Edward L.G. 
Published: February 2012

SummaryThe incorporation of virus- and host-derived procoagulant factors initiates clotting directly on the surface of herpesviruses, which is an explanation for their correlation to vascular disease. The virus exploits the resulting thrombin to enhance infection by modulating the host cell through protease activated receptor (PAR) 1 signalling. Prior reports demonstrated that at least one herpesvirus expresses surface annexin A2 (A2), a cofactor for tissue plasminogen activator (tPA)-dependent activation of plasminogen to plasmin. Since plasmin is both a fibrinolytic protease and PAR agonist, we investigated whether herpesviruses enhance fibrinolysis and the effect of plasmin on cell infection. Herpes simplex virus types 1 (HSV1) and 2, and cytomegalovirus (CMV) purified from various cell lines each accelerated the proteolytic activation of plasminogen to plasmin by tPA. Ligand blots identified A2 as one of several plasminogen binding partners associated with the virus when compared to an A2-deficient virus. This was confirmed with inhibitory A2-antibodies. However, A2 was not required for virus-enhanced plasmin generation. HSV1, HSV2 and CMV accelerated tPA-dependent fibrin clot lysis by up to 2.8-fold. Modest plasmin generation and fibrinolysis was detected independent of exogenous tPA, which was inhibited by plasminogen activator inhibitor type-1 and ε-aminocaproic acid; however, the molecular basis remains speculative. Up to a ∼6-fold enhancement of infection was provided by plasmin-mediated cell infection. Inhibitory antibodies revealed that plasmin increased HSV1 infection through a mechanism involving PAR2. Thus, virus-enhanced fibrinolysis may help explain the paradox of the highly procoagulant in vitro herpesvirus surface eliciting only relatively weak independent vascular disease risk.
MicroRNAs in the regulation of immune cell functions – implications for atherosclerotic vascular disease
Authors: Zernecke Alma 
Published: February 2012

SummaryRegarded as a chronic inflammatory disease of the vessel wall, the development of atherosclerotic lesions is shaped by immune responses and their regulation. Macrophages and dendritic cells are positioned at the crossroad of innate and adaptive immune responses by sensing atherogenic danger signals and by taking up and presenting antigens. T helper cells and auto-antibodies produced by B cells, together with their cytokine responses in turn modulate atheroprogression. In addition, platelets contribute to atherosclerosis by multiple pathways. microRNAs (miRNAs) that post-transcriptionally regulate gene expression may thus critically control immune cell differentiation and functions during plaque evolution. This review summarises the role of miRNAs in regulating lipid uptake and expression of inflammatory mediators in monocytes/macrophages and dendritic cells, in lymphocyte functions with a focus on T helper cell responses, as well as in platelet biology, and the implications of altering these functions in vascular pathology and atherosclerosis. T systematically survey miRNA functions in controlling molecular mechanisms and immune responses in atherosclerosis holds potential for the development of novel miRNA-based strategies for therapies targeting inflammation and immunity in atherosclerosis.
Factors associated with failure to correct the international normalised ratio following fresh frozen plasma administration among patients treated for warfarin-related major bleeding
Authors: White Leigh , Friedman Mark , Nichols Christine , Menzin Jordan , Hoesche Jan , Bergman Garrett E. , Jones Christopher , Menzin Joseph 
Published: February 2012

SummaryThis study assessed the frequency and factors associated with failure to correct international normalised ratio (INR) in patients administered fresh frozen plasma (FFP) for warfarin-related major bleeding. This retrospective database analysis used electronic health records from an integrated health system. Patients who received FFP between 01/01/2004 and 01/31/2010, and who met the following criteria were selected: major haemorrhage diagnosis the day before to the day after initial FFP administration; INR ≥2 on the day before or the day of FFP and another INR result available; warfarin prescription within 90 days. INR correction (defined as INR ≤1.3) was evaluated at the last available test up to one day following FFP. A total of 414 patients met selection criteria (mean age 75 years, 53% male, mean Charlson score 2.5). Patients presented with gastrointestinal bleeding (58%), intracranial haemorrhage (38%) and other bleed types (4%). The INR of 67% of patients remained uncorrected at the last available test up to one day following receipt of FFP. In logistic regression analysis, the INR of patients who were older, those with a Charlson score of 4 or greater, and those with non-ICH bleeds (odds ratio vs. intracranial bleeding 0.48; 95% confidence interval 0.31–0.76) were more likely to remain uncorrected within one day following FFP administration. In an alternative definition of correction, (INR ≤1.5), 39% of patients’ INRs remained uncorrected. For a substantial proportion of patients, the INRs remain inadequately or uncorrected following FFP administration, with estimates varying depending on the INR threshold used.
Is EPCR a multi-ligand receptor? Pros and cons
Authors: Molina Eva , Hermida José , Montes Ramón , Puy Cristina 
Published: February 2012

SummaryIn the last decade, the endothelial cell protein C/activated protein C receptor (EPCR) has received considerable attention. The role initially attributed to EPCR, i.e. the enhancement of protein C (PC) activation by the thrombin-thrombomodulin complex on the surface of the large vessels, although important, did not go beyond the haemostasis scenario. However, the discovery of the cytoprotective, anti-inflammatory and anti-apoptotic features of the activated PC (APC) and the required involvement of EPCR for APC to exert such actions did place the receptor in a privileged position in the crosstalk between coagulation and inflammation. The last five years have shown that PC/APC are not the only molecules able to interact with EPCR. Factor VII/VIIa (FVII/VIIa) and factor Xa (FXa), two other serine proteases that play a central role in haemostasis and are also involved in signalling processes influencing wound healing, tissue remodelling, inflammation or metastasis, have been reported to bind to EPCR. These observations have paved the way for an exploration of unsuspected new roles for the receptor. This review aims to offer a new image of EPCR in the light of its extended panel of ligands. A brief update of what is known about the APC-evoked EPCR-dependent cell signalling mechanisms is provided, but special care has been taken to assemble all the information available about the interaction of EPCR with FVII/VIIa and FXa.
Impact of the Dual-Polarization Doppler Radar Data on Two Convective Storms with a Warm-Rain Radar Forward Operator
Authors: Li Xuanli , Mecikalski John R. 
Published: February 2012

AbstractThe dual-polarization (dual pol) Doppler radar can transmit/receive both horizontally and vertically polarized power returns. The dual-pol radar measurements have been shown to provide a more accurate precipitation estimate compared to traditional radars. In this study, the horizontal reflectivity ZH, differential reflectivity ZDR, specific differential phase KDP, and radial velocity VR collected by the C-band Advanced Radar for Meteorological and Operational Research (ARMOR) are assimilated for two convective storms. A warm-rain scheme is constructed to assimilate ZH, ZDR, and KDP data using the three-dimensional variational data assimilation (3DVAR) system with the Advanced Research Weather Research and Forecasting Model (ARW-WRF). The main goals of this study are first to demonstrate and compare the impact of various dual-pol variables in initialization of real case convective storms and second to test how the dual-pol fields may be better used with a 3DVAR system.The results show that the ZH, ZDR, KDP, and VR data substantially improve the initial condition for two mesoscale convective storms. Significant positive impacts on short-term forecast are obtained for both storms. Additionally, KDP and ZDR data assimilation is shown to be superior to ZH and ZDR and ZH-only data assimilation when the warm-rain microphysics is adopted. With the ongoing upgrade of the current Weather Surveillance Radar-1988 Doppler (WSR-88D) network to include dual-pol capabilities (started in early 2011), the findings from this study can be a helpful reference for utilizing the dual-pol radar data in numerical simulations of severe weather and related quantitative precipitation forecasts.
The Diurnal Cycle of Precipitation from Continental Radar Mosaics and Numerical Weather Prediction Models. Part II: Intercomparison among Numerical Models and with Nowcasting
Authors: Berenguer Marc , Surcel Madalina , Zawadzki Isztar , Xue Ming , Kong Fanyou 
Published: February 2012

Abstract
This second part of a two-paper series compares deterministic precipitation forecasts from the Storm-Scale Ensemble Forecast System (4-km grid) run during the 2008 NOAA Hazardous Weather Testbed (HWT) Spring Experiment, and from the Canadian Global Environmental Multiscale (GEM) model (15 km), in terms of their ability to reproduce the average diurnal cycle of precipitation during spring 2008. Moreover, radar-based nowcasts generated with the McGill Algorithm for Precipitation Nowcasting Using Semi-Lagrangian Extrapolation (MAPLE) are analyzed to quantify the portion of the diurnal cycle explained by the motion of precipitation systems, and to evaluate the potential of the NWP models for very short-term forecasting.
The observed diurnal cycle of precipitation during spring 2008 is characterized by the dominance of the 24-h harmonic, which shifts with longitude, consistent with precipitation traveling across the continent. Time–longitude diagrams show that the analyzed NWP models partially reproduce this signal, but show more variability in the timing of initiation in the zonal motion of the precipitation systems than observed from radar.
Traditional skill scores show that the radar data assimilation is the main reason for differences in model performance, while the analyzed models that do not assimilate radar observations have very similar skill.
The analysis of MAPLE forecasts confirms that the motion of precipitation systems is responsible for the dominance of the 24-h harmonic in the longitudinal range 103°–85°W, where 8-h MAPLE forecasts initialized at 0100, 0900, and 1700 UTC successfully reproduce the eastward motion of rainfall systems. Also, on average, MAPLE outperforms radar data assimilating models for the 3–4 h after initialization, and nonradar data assimilating models for up to 5 h after initialization.
Effect of Boot Weight and Sole Flexibility on Gait and Physiological Responses of Firefighters in Stepping Over Obstacles
Authors: Chiou Sharon S. , Turner Nina , Zwiener Joyce , Weaver Darlene L. , Haskell William E. 
Published: February 2012

Objective: The authors investigated the effect of boot weight and sole flexibility on spatiotemporal gait characteristics and physiological responses of firefighters in negotiating obstacles. Background: Falls and overexertion are the leading causes of fire ground injuries and fatalities among firefighters. There have been few in-depth studies conducted to evaluate the risk factors of falls and overexertion associated with firefighter boots. Method: For the study, 13 female and 14 male firefighters, while wearing full turnout clothing and randomly assigned boots, walked for 5 min while stepping over obstacles. The independent variables included boot weight, sole flexibility, gender, and task duration. Spatiotemporal measures of foot trajectories and toe clearance were determined. Minute ventilation, oxygen consumption, carbon dioxide production, and heart rate were measured. Results: Increased boot weight was found to significantly reduce trailing toe clearance when crossing the 30-cm obstacle. Significant increases in lateral displacement of the foot were found near the end of the 5-min walk compared with the beginning of the task. Increased boot weight significantly increased oxygen consumption. There were significant decreases in oxygen consumption for more flexible soles. Conclusion: Firefighters were more likely to trip over obstacles when wearing heavier boots and after walking for a period of time. Boot weight affected metabolic variables (5% to 11% increases per 1-kg increase in boot weight), which were mitigated by sole flexibility (5% to 7% decrease for more flexible soles). Application: This study provides useful information for firefighters and boot manufacturers in boot selection and design for reducing falls and overexertion.
Toxic effects of some synthetic food colorants and/or flavor additives on male rats
Authors: El-Wahab Hanan Mohamed Fathy Abd , Moram Gehan Salah El-Deen 
Published: February 2012

The objective of the present work was to evaluate the broadest toxic effect of some synthetic additives of colorants and/or flavors on different body organs and metabolic aspects in rats.A number of chemical food color and flavor additives are routinely added during processing to improve the aesthetic appearance of the dietary items. However, many of them are toxic after prolonged use. In this experiment, a total of 100 male albino rats of Spargue Dawley strain were divided into 10 groups: G1 was fed basal diet and served as control, G2: basal diet + Brilliant blue (blue dye, No. 2, 124 mg/kg diet), G3: basal diet + carmoisine (red dye, No. 3, 70 mg/kg diet), G4: basal diet + tartrazine (yellow dye, FD & C yellow No. 5, 75 mg/kg diet), G5: basal diet + trans-anethole (4.5 g/kg diet) G6: basal diet + propylene glycol (0.25 g/kg diet), G7: basal diet + vanillin(1.25 g/kg diet), G8: basal diet + Brilliant blue + propylene glycol, G9: basal diet + carmoisine + trans-anethole, G10: basal diet + tartrazine + vanillin for 42 successive days. All food colorants mixed with or without flavor additives induced a significant decrease in body weight, hemoglobin concentration and red blood cell count. Also there was a significant decrease in reduced glutathione content; glutathione- S-transferase and superoxide dismutase activities in both blood and liver compared to control group. On the other hand, a significant increase in serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase activities, bilirubin, urea, creatinine, total protein and albumin were observed in all test groups when compared to control group. Finally, it is advisable to limit the uses of these food colorants and/or food flavor additives especially those used by children.
Determination of protective role of selenium against aflatoxin B1-induced DNA damage
Authors: Agar Guleray , Alpsoy Lokman , Bozari Sedat , Erturk Filiz Aygun , Yildirim Nalan 
Published: February 2012

Selenium is an essential mineral for a healthy life. Appropriate doses of it may undertake a protective role in the organism. In this study, the protective role of selenium (Se4+) against aflatoxin B1 (AFB1)-induced DNA damage was determined using random amplified polymorphic DNA on two plants including Vicia faba and Zea mays. It was observed that the concentrations of 0.1, 0.2 and 0.4 ppm of AFB1 have increased polymorphism value, total chlorophyll inhibition rate (IRc, %) and total protein IR (IRp, %). Unlike protein, chlorophyll contents and genomic template stability were decreased. With the addition of different concentrations (0.8 and 80 ppm) of Se4+ to the treated samples with AFB1, the values return to normal. An 800-ppm concentration of Se4+, on the contrary, could not inhibit the toxicity of AFB1 but caused an increase in toxicity level of AFB1/enhanced the toxicity level of AFB1. Results suggested that Se4+ has an antagonistic effect against AFB1 toxicity and that the degree of antagonistic effect of Se4+ against AFB1 was related to its concentration.
Rape and Spiritual Death
Authors: Messina-Dysert Gina 
Published: February 2012

Rape is a form of violence that causes destructive consequences to both the physical and spiritual health of women. Due to its taboo nature as well as the societal response to the victim, rape is especially harmful and results in han, a Korean concept that signifies a compressed suffering. The continual torment caused by han damages the rape victim’s spiritual health and ultimately leads to spiritual death. This article offers a definition of spiritual death and explores how the experience of the violent act of rape within a rape culture constructs han. Specifically, the victims’ inability to express pain as well as assumed qualities of shame, self blame, and invisibility are examined with a focus on the societal response’s ability to provoke these qualities, further contributing to the emotional and spiritual trauma which results in a spiritual death. The possibility for multiple spiritual resurrections through various practices including the application of the traditional practice of Han-Pu-Ri, a Korean shamanistic ritual that releases han is discussed.
Occupational therapy and sensory integration for children with autism: a feasibility, safety, acceptability and fidelity study
Authors: Schaaf Roseann C. , Benevides Teal W. , Kelly Donna , Mailloux-Maggio Zoe 
Published: February 2012

Objective: To examine the feasibility, safety, and acceptability of a manualized protocol of occupational therapy using sensory integration principles for children with autism. Methods: Ten children diagnosed with autism spectrum disorder ages 4-8 years received intensive occupational therapy intervention using sensory integration principles following a manualized protocol. Measures of feasibility, acceptability and safety were collected from parents and interveners, and fidelity was measured using a valid and reliable fidelity instrument. Results: The intervention is safe and feasible to implement, acceptable to parents and therapist, and therapists were able to implement protocol with adequate fidelity. These data provide support for implementation of a randomized control trial of this intervention and identify specific procedural enhancements to improve study implementation.
Veterans service organization engagement in ‘POWER,’ a peer-led hypertension intervention
Authors: Mosack Katie E , Wendorf Angela R , Brouwer Amanda M , Patterson Leslie , Ertl Kristyn , Whittle Jeff , Morzinski Jeff , Fletcher Kathlyn 
Published: February 2012

Objectives: The purpose of this study was to determine the influence of program factors on participant engagement in POWER, a peer-led intervention designed to reduce hypertension, increase hypertension knowledge, and improve other relevant health behaviors, such as diet and exercise, among US veterans involved in veterans service organizations throughout Southeastern Wisconsin. Methods: Two hundred and nineteen hypertensive members from 58 VSOs participated in a year-long peer-led intervention designed to improve hypertension knowledge, disease self-management behaviors, and health outcomes. This study represents a qualitative evaluation of post and participant engagement in this intervention. We triangulated data collected via three qualitative approaches (observations, focus groups, and in-depth interviews) from intervention posts to derive a model of engagement. Results: Our findings indicate that discrete characteristics of the peer leaders, post members, posts, and the intervention itself contributed to intervention engagement. Discussion: We make suggestions for future research studies, particularly as related to understanding how peer leader identities and cultural norms within VSOs might contribute to peer-led health intervention success.