bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluating the polar organic chemical integrative sampler for the monitoring of beta-blockers and hormones in wastewater treatment plant effluents and receiving surface waters
Authors: Jacquet Romain , Miège Cécile , Bados Philippe , Schiavone Séverine , Coquery Marina 
Published: November 2011

Abstract
Wastewater treatment plants (WWTP) are known to be a source of surface water contamination by organic compounds such as pharmaceuticals. The objective of the present work was to study the suitability of the polar organic chemical integrative sampler (POCIS) to monitor beta-blockers and hormones in effluents and surface waters. Four sampling campaigns were carried out in French rivers (the Saône, the Ardières, the Bourbre, and the Seine) between November 2007 and September 2008. Passive samplers were exposed in surface waters, upstream and downstream of WWTP outflows, and in effluents. Exposures lasted for up to 24 d to study the uptake kinetics directly in situ, and repeatability was assessed by exposure of triplicates. A good agreement was found between POCIS and water samples. With the exception of atenolol, beta-blockers showed a linear uptake during at least three weeks, and their sampling rates could be determined in situ. These sampling rates were then used to calculate time-weighted average concentrations of beta-blockers in the Seine River with an overall good accuracy and repeatability. Such calculations could not be performed for hormones because of their variable occurrences and low concentrations in water and POCIS. Polar organic chemical integrative sampler therefore seems to be a suitable tool for monitoring beta-blockers in surface waters impacted by WWTP effluents. Longer exposure durations would be necessary to determine the suitability of POCIS for monitoring hormones. Finally, preliminary assays on the use of several deuterated compounds as performance reference compounds showed promising results for deuterated atenolol. Environ. Toxicol. Chem. 2012;31:279–288. © 2011 SETAC
From [10]Paracyclophane to Ferrocenophanones: The Search for Molecular Machines and Bio‐Organometallic Anticancer Drugs
Authors: McGlinchey Michael J. , Milosevic Sandra 
Published: November 2011

AbstractA partial quenching of the NMR ring current in [10]paracyclophane‐chromium tricarbonyl prompted a study of other metal–arene π complexes, several of which exhibited restricted intramolecular motion relevant to their potential use in molecular machines. An attempted Diels–Alder reaction of 9‐phenylethynyl‐9H‐fluorene with tetracyclone instead yielded a novel tetracene by isomerization of the alkyne to the corresponding allene, and then via a series of allene dimers which are classifiable as cyclophanes. (Subsequently, the first organometallic molecular brake was prepared, whereby migration of a metal carbonyl tripod over an indenyl framework blocked the rotation of a triptycene paddlewheel.) Cyclophanes have now found applicability in the field of bio‐organometallic chemistry; the activity of tamoxifen, the first line treatment for hormone‐dependent breast cancers, is markedly enhanced when the structure is modified by incorporation of a ferrocenophane moiety. Finally, we relate the story of how the first cyclophane, [1.1.1]orthocyclophane, was actually prepared by Cannizzaro in 1854, but was only recognized as such more than 150 years later.
Increased expression of the histamine H4receptor subtype in hypertrophic differentiation of chondrogenic ATDC5 cells
Authors: Yamaura Katsunori , Akiyama Seri , Ueno Koichi 
Published: November 2011

AbstractHistamine has been regarded as an inflammatory mediator of arthritic disorders. We have previously reported that the expression of histamine H4receptor (H4R) mRNA in synovial tissues was significantly higher in patients with osteoarthritis (OA) compared to those with rheumatoid arthritis. Chondrocyte hypertrophy and endochondral ossification are essential processes in pathologic disorders such as osteophyte formation during OA progression. In the present study, we examined the expression of H4R during differentiation into hypertrophic chondrocytes in the ATDC5 cells, a widely used in vitro model of chondrogenic differentiation. Quantitative reverse transcription polymerase chain reaction showed that the levels of histidine decarboxylase and H4R mRNA on ATDC5 cells were increased in a time‐dependent manner during the culture period. By contrast, the expressions of H1R and H2R were not increased from day 7 onwards. The mRNA expression of the hypertrophic chondrocyte marker type X collagen (COL X) was increased markedly from 14 to 21. Immunocytochemical analysis indicated that H4R staining was strongly immunoreactive on the plasma membrane of ATDC5 cells. Flow cytometry showed increased expression of H4R and COL X protein in ATDC5 chondrocytes. Furthermore, the majority of the COL X‐positive cells expressed H4R throughout the culture period. In summary, we showed for the first time that H4R is expressed in ATDC5 chondrocytes. Moreover, we found that most hypertrophic chondrocytes express H4R, suggesting that this receptor might be associated with the differentiation of chondrocytes into hypertrophic cells, which are abnormally observed in joint lesions in OA. J. Cell. Biochem. 113: 1054–1060, 2012. © 2011 Wiley Periodicals, Inc.
B‐flow imaging for the measurement of residual lumen diameter of renal artery stenosis
Authors: Tola Muharrem , Yurdakul Mehmet , Ozbulbul Nilgün Isiksalan 
Published: November 2011

AbstractBackground.The aim of this study was to assess the accuracy and efficacy of B‐flow imaging (BFI) in the diagnosis and measurement of renal artery stenosis (RAS) compared with color duplex ultrasonography (CDU) and digital subtraction angiography (DSA).Methods.Fifty‐one consecutive patients with RAS diagnosed and measured with DSA were subsequently and independently examined with BFI for the measurement of residual lumen diameter, and with CDU for the measurement of peak systolic velocity and renal‐aortic velocity ratio. The diagnostic performances of BFI and CDU in determining 60–99% RAS were compared by receiver operating characteristic curve analysis. The agreement between DSA and BFI stenosis measurements was evaluated with Bland‐Altman method.Results.The area under curve was 0.983 for BFI and 0.959 for CDU, without a significant difference in diagnostic performances (p = 0.26). BFI yielded an 88% sensitivity and 94% specificity in the diagnosis of RAS with a 3% underestimation of RAS compared to DSA (95% confidence interval 1.4%, 4.6%).Conclusions.BFI is an accurate method that minimally underestimates RAS. It might provide an additional benefit to CDU in patients with RAS. © 2011 Wiley Periodicals, Inc. J Clin Ultrasound 40:85–90, 2012
Diffusion‐weighted whole‐body MRI with background body signal suppression: Technical improvements at 3.0 T
Authors: Mürtz Petra , Kaschner Marius , Träber Frank , Kukuk Guido , Skowasch Dirk , Gieseke Jürgen , Schild Hans H. , Willinek Winfried A. 
Published: November 2011

AbstractPurpose:To improve image quality of diffusion‐weighted body magnetic resonance imaging (MRI) with background body signal suppression (DWIBS) at 3.0 T.Materials and Methods:In 30 patients and eight volunteers, a diffusion‐weighted spin‐echo echo‐planar imaging sequence with short TI inversion recovery (STIR) fat suppression was applied and repeated using slice‐selective gradient reversal (SSGR) and/or dual‐source parallel radiofrequency (RF) transmission (TX). The quality of diffusion‐weighted images and gray scale inverted maximum intensity projections (MIP) were visually assessed by intraindividual comparison with respect to the level of fat suppression and signal homogeneity. Moreover, the contrast between lesions/lymph nodes and background (Clb) was analyzed in the MIP reconstructions.Results:By combining STIR with SSGR, fat suppression was significantly improved (P < 0.001) and Clb was increased two times. The use of TX allowed the reduction of acquisition time and improved image quality with regard to signal homogeneity (P < 0.001) and fat suppression (P = 0.005).Conclusion:DWIBS at 3.0 T can be improved by using SSGR and TX. J. Magn. Reson. Imaging 2012;456‐461. © 2011 Wiley Periodicals, Inc.
Imaging modalities of gastrointestinal stromal tumors
Authors: Choi Haesun 
Published: November 2011

AbstractRecent dramatic improvement of survival of Gastrointestinal stromal tumor patients alongside the development of a new targeted treatment has made the role of imaging increasingly more important not only in diagnosing the disease, but also in monitoring the treatment effect and surveillance. Computed tomography is currently the imaging modality of choice for these purposes. This article reviews the current roles of different imaging modalities in clinical management of the gastrointestinal stromal tumors. J. Surg. Oncol. 2011; 104:907–914. © 2011 Wiley Periodicals, Inc.
Advanced or metastatic gastrointestinal stromal tumors: Systemic treatment options
Authors: Caram Megan V. , Schuetze Scott M. 
Published: November 2011

AbstractGastrointestinal stromal tumor (GIST), the most common sarcoma arising in the gastrointestinal tract, typically expresses the tyrosine‐kinase receptor, C‐KIT, and contains activating mutation in the c‐kit or platelet‐derived growth factor receptor (pdgfr) gene. Recently, development of small molecules that inhibit the kinase activity of mutant C‐KIT and PDGFR proteins has radically changed treatment and prognosis of patients diagnosed with advanced GIST as this molecularly “targeted” therapy has demonstrated remarkable high‐level of activity in this disease. J. Surg. Oncol. 2011; 104:888–895. © 2011 Wiley Periodicals, Inc.
A prognostic model to predict outcome of patients failing to achieve pathological complete response after anthracycline‐containing neoadjuvant chemotherapy for breast cancer
Authors: Chen Sheng , Chen Can‐Ming , Yu Ke‐Da , Yang Wen‐Tao , Shao Zhi‐Ming 
Published: November 2011

AbstractBackgroundThe aim of this study was to evaluate factors that could possibly affect the outcome of patients failing to achieve pathological complete response (pCR) after anthracycline‐containing neoadjuvant chemotherapy (NCT) for breast cancer, and built a prognostic model to predict patients' outcome.Patients and MethodsData from 199 stage II–III breast cancer patients who failed to achieve pCR after NCT were used. Variables at baseline and at surgery (age, menopausal status, tumour size, grade, histotype, node status, vascular invasion, ER, PR, HER‐2, Cathepsin D, P53, Topo‐IIα, Nm‐23, Bcl‐2, BAX, MDR, GSTN, PS2, P27, Cyclin D1 and Ki‐67) were investigated.ResultsTumour marker Ki‐67, Cathepsin D status and number of positive lymph nodes at surgery were significant prognostic factors in multivariate analysis for both DFS and OS. According to our prognostic model, the 5‐year DFS rates in low, intermediate‐low, intermediate‐high and high‐risk groups were 94%, 65%, 43% and 28%, respectively (log‐rank test P < 0.001). The 5‐year OS rates in these four groups were 94%, 84%, 66% and 34%, respectively (log‐rank test P < 0.001).ConclusionOur prognostic model could easily discriminate patients with different risks of experiencing an event or death, which could allow physicians to tailor treatment strategies specifically and individually. J. Surg. Oncol. 2012; 105:577–585. © 2011 Wiley Periodicals, Inc.
Molecularly imprinted solid‐phase extraction for the selective HPLC determination of ractopamine in pig urine
Authors: Zhang Qingjie , Su Yijuan , He Qianqian , Shen Xianguang , He Limin , Zhang Nan , Zeng Zhenling 
Published: November 2011

AbstractA method was developed for the determination of ractopamine in pig urine using molecularly imprinted solid‐phase extraction (MISPE) as the sample clean‐up technique combined with high‐performance liquid chromatography. The molecularly imprinted polymer (MIP) was synthesized in acetonitrile–triethylamine system using ractopamine (RAC) as the template and acrylamide as the monomer. The binding capacity of the polymer toward RAC was found to be about 2.57 mg of ractopamine/g of polymer. The optimal procedures for MISPE consisted of conditioning with 3 mL methanol, equilibrating with 3 mL of water, loading volume of <10 mL of aqueous sample (pH 7), washing with 3 mL water and 3 mL methanol, and eluting with 5 mL of 5% ammonia in methanol. In the four spiked samples with the levels of 0.01, 0.1, 1.0 and 5.0 μg/mL, the mean recoveries of analyte on the MIP were higher than 90% with relative standard deviation <10%, and significant differences between imprinted and non‐imprinted materials were observed. The MIP selectivity was evaluated by checking 11 drugs with similar and different molecular structures to that of RAC. The characteristics of three‐dimensional cavities and hydrogen bond interaction were regarded as the main factors that dominated the retention of RAC on the MISPE cartridge.
Misleading correlations: The case of the Canada warbler and spruce budworm
Authors: Venier Lisa A. , Holmes Stephen B. , Pearce Jennie L. , Fournier Ronald E. 
Published: November 2011

AbstractIn a recently published article Sleep et al. (2009) suggested that 30‐yr declines in Canada warbler (1975–2005) based on Breeding Bird Survey (BBS) data could be attributed to 30‐yr declines in spruce budworm through a series of analyses based principally on correlations. We demonstrate that the relationship does not hold when a longer‐term data set (1968–2008) is used for the analysis. Sleep et al. (2009) also demonstrated a positive relationship between correlations of Canada warbler abundance and time and spruce budworm defoliation and time using provincial data sets. We examined the underlying BBS data and found that there were insufficient observations of Canada warbler in the western provinces (Alberta and Manitoba) to support the conclusion because most Canada warbler observations occurred far (>100 km) from any budworm defoliation. As well, we used a density‐dependent stochastic population growth model as proposed by Sleep et al. (2009) and found only 2 significant relationships (Nova Scotia, Alberta) between Canada warbler population growth rate and budworm defoliation at the provincial scale. We conclude that little analytical evidence exists to support the idea that Canada warbler decline is a function of spruce budworm decline. © 2011 The Wildlife Society.
Using echolocation monitoring to model bat occupancy and inform mitigations at wind energy facilities
Authors: Weller Theodore J. , Baldwin James A. 
Published: November 2011

AbstractFatalities of migratory bats, many of which use low frequency (<35 kHz; LowF) echolocation calls, have become a primary environmental concern associated with wind energy development. Accordingly, strategies to improve compatibility between wind energy development and conservation of bat populations are needed. We combined results of continuous echolocation and meteorological monitoring at multiple stations to model conditions that explained presence of LowF bats at a wind energy facility in southern California. We used a site occupancy approach to model nightly LowF bat presence while accounting for variation in detection probability among echolocation detectors and heights. However, we transposed the spatial and temporal axes of the conventional detection history matrix such that occupancy represented proportion of nights, rather than monitoring points, on which LowF bats were detected. Detectors at 22 m and 52 m above ground had greater detection probabilities for LowF bats than detectors at 2 m above ground. Occupancy of LowF bats was associated with lower nightly wind speeds and higher nightly temperatures, mirroring results from other wind energy facilities. Nevertheless, we found that building separate models for each season and considering solutions with multiple covariates resulted in better fitting models. We suggest that use of multiple environmental variables to predict bat presence could improve efficiency of turbine operational mitigations (e.g., changes to cut‐in speeds) over those based solely on wind speed. Increased mitigation efficiencies could lead to greater use of mitigations at wind energy facilities with benefits to bat populations. © 2011 The Wildlife Society.
Understanding the Mechanism of Anomalous Viscosity–Molecular Weight Relationships of Diolic Perfluoropoly (Oxyethylene‐ran‐oxymethylene): What is Missing in the Debye–Bueche Model?
Authors: Wang Yongjin , Williams Kara T. , Li Lei 
Published: November 2011

AbstractFor decades, the viscosity (η)–molecular weight (M) relationship of linear polymer melts has been successfully described by the Debye–Bueche model when M is lower than critical molecular weight (Mc). However, recent studies on diolic perfluoropoly (oxyethylene‐ran‐oxymethylene) (DPFPO), a linear polymer with polar endgroups, raise uncertainty on the validity of Debye–Bueche model. Experimental results show that the Debye–Bueche model does not hold for DPFPO, which has been attributed to the fact that the strong intermolecular endgroup–endgroup coupling is not considered in original Debye–Bueche model. A modified molecular‐level model, in which the endgroup–endgroup coupling is taken into consideration, is proposed to interpret the anomalous η–M relationship of DPFPO.
Expression of GATA1 in the ovine conceptus and endometrium during the peri‐attachment period
Authors: Bai Hanako , Sakurai Toshihiro , Konno Toshihiro , Ideta Atsushi , Aoyagi Yoshito , Godkin James D. , Imakawa Kazuhiko 
Published: November 2011

AbstractThe transcription factor GATA1 is known to play an essential role in hematopoiesis, but its other roles have not been well characterized. The purpose of this study was to determine relationships between GATA1 and GATA2 and/or GATA3, and to identify their possible functions in ovine development. GATA1 mRNA was found in ovine conceptuses and endometrial epithelial regions of Day 15 (Day 0 = day of estrus) cyclic and Days 15, 17, and 21 pregnant ovine uteri. GATA1 mRNA was strongly expressed in conceptuses on Day 21, when trophoblast attachment to the maternal endometrium progressed. Similarly, GATA1 protein expression was relatively high on Day 21. To localize GATA1 mRNA, ovine conceptuses and pregnant uteri were subjected to in situ hybridization on Days 15, 17, and 21, confirming that GATA1 mRNA was expressed in trophoblasts and uterine endometrial epithelial cells in these gestation days. The presence of GATA1 protein was further confirmed by immunohistochemistry. Because high GATA1 expression appeared to coincide with reduced GATA2/3 expression, a potential role of GATA1 was examined through transfection of a mouse Gata1 expression plasmid into bovine trophoblast F3 cells. This over‐expression resulted in the down‐regulation of endogenous GATA2 transcripts. These observations indicate that GATA1 exists in the ovine conceptus and uterus during the peri‐attachment period, and suggest that GATA1 is integral to conceptus and endometrial development through the regulation of GATA2 and possibly other developmentally important genes. Mol. Reprod. Dev. 79:64–73, 2012. © 2011 Wiley Periodicals, Inc.
Quasikristalle — Schnittstellen zum Hyperraum
Authors: Steurer Walter 
Published: November 2011

AbstractAls der israelische Wissenschaftler Dan Shechtman am 8. April 1982 Elektronenbeugungsbilder seiner rasch abgeschreckten Al‐Mn‐Legierungen durchsah, traute er seinen Augen nicht — er sah ein zehnzähliges Beugungsbild mit scharfen Bragg‐Reflexen.
CE ergänzt HPLC: Kapillarelektrophorese‐Massenspektrometrie
Authors: Grundmann Marco , Matysik Frank‐Michael 
Published: November 2011

AbstractBesonders für Biomoleküle ist die Kapillarelektrophorese eine effektive und unaufwendige Alternative zur HPLC. Auch die Kapillarelektrophorese lässt sich mit Massenspektrometrie koppeln und ergänzt für geladene Analyte die HPLC.
Partial equilibrium in risk‐based production decisions
Authors: Kamrad Bardia , Siddique Akhtar , Ernst Ricardo 
Published: November 2011

AbstractCharacteristically, a small subset of operational problems admit risk neutrality when contingent claims methodology were used in their analysis. That is, for the majority of manufacturing and production problems, operating cash flows are not directly linked to prices of traded assets. However, to the extent that correlations can be estimated, the methodology's applicability to a broader set of operational problems is supported. Our article addresses this issue with the objective of extending the use of contingent claims techniques to a larger set of operational problems. In broad terms, this objective entails a partial equilibrium approach to the problem of valuing uncertain cash flows. To this end, we assume risk aversion and cast our approach within Merton's intertemporal capital asset pricing model. In this context, we formulate a “generic” production valuation model that is framed as an exercise in stochastic optimal control. The model is versatile in its characterization and can easily be adapted to accommodate a wide‐ranging set of risk‐based operational problems where the underlying sources of uncertainty are not traded. To obtain results, the model is recast as a stochastic dynamic program to be solved numerically. The article addresses a number of fundamental issues in the analysis risk based decision problems in operations. First, in the approach provided, decisions are analyzed under a properly defined risk structure. Second, the process of analysis leads to suitably adjusted probability distributions through which, appropriately discounted expectations are derived. Third, through consolidating existing concepts into a standard and adaptable framework, we extend the applicability of contingent claims methodology to a broader set of operational problems. The approach is advantageous as it obviates the need for exogenously specifying utility functions or discount rates.© 2011 Wiley Periodicals, Inc. Naval Research Logistics, 2011
The nuclear proteome of the green alga Chlamydomonas reinhardtii
Authors: Winck Flavia V. , Riaño‐Pachón Diego M. , Sommer Frederik , Rupprecht Jens , Mueller‐Roeber Bernd 
Published: November 2011

AbstractNuclear proteins play a central role in regulating gene expression. Their identification is important for understanding how the nuclear repertoire changes over time under different conditions. Nuclear proteins are often underrepresented in proteomic studies due to the frequently low abundance of proteins involved in regulatory processes. So far, only few studies describing the nuclear proteome of plant species have been published. Recently, the genome sequence of the unicellular green alga Chlamydomonas reinhardtii has been obtained and annotated, allowing the development of further detailed studies for this organism. However, a detailed description of its nuclear proteome has not been reported so far. Here, we present an analysis of the nuclear proteome of the sequenced Chlamydomonas strain cc503. Using LC‐MS/MS, we identified 672 proteins from nuclei isolates with a maximum 1% peptide spectrum false discovery rate. Besides well‐known proteins (e.g. histones), transcription factors and other transcriptional regulators (e.g. tubby and HMG) were identified. The presence of protein motifs in nuclear proteins was investigated by computational tools, and specific over‐represented protein motifs were identified. This study provides new insights into the complexity of the nuclear environment and reveals novel putative protein targets for further studies of nuclear mechanisms.