bluuebunny/crossref_metadata_2025_split huggingface dataset:
The road to culturally relevant science: Exploring how teachers navigate change in pedagogy
Authors: Johnson Carla C. 
Published: December 2010

AbstractIn this study, two middle‐school teachers who participated in a professional development program utilizing the transformative professional development (TPD) model are followed as they embarked upon becoming culturally relevant science teachers of Hispanic students. Using Ladson‐Billings (1994) theory of culturally relevant pedagogy, teacher interviews, focus groups, journals, and field notes are examined to reveal aspects of culturally relevant pedagogy that the participants translate into their daily science instructional practice in this longitudinal case study. Findings revealed TPD enabled participants to transform their practice to focus on culturally relevant science pedagogy resulting in a more effective instructional environment for their Hispanic students. Implications for further research on professional development and other supports for teachers integrating culturally relevant pedagogy are discussed. © 2010 Wiley Periodicals, Inc., J Res Sci Teach 48: 170–198, 2011
An empirical investigation of scheduling performance criteria
Authors: De Snoo Cees , Van Wezel Wout , Jorna René J. 
Published: December 2010

AbstractPlanning and scheduling significantly influence organizational performance, but literature that pays attention to how organizations could or should organize and assess their planning processes is limited. We extend planning and scheduling theory with a categorization of scheduling performance criteria, based on a three‐stage survey research design. Particularly, the results show that, next to schedule quality, the planning process factors timeliness, flexibility, communication, and negotiation are important performance criteria, and especially so in organizations that are faced with high levels of uncertainty. The results suggest that organizational and behavioral aspects of planning and scheduling cannot be mitigated with advanced models and software that solely focus on good schedules. Rather, high quality schedules and high quality scheduling processes need to be facilitated simultaneously to attain high planning and scheduling performance.
Transpiration in plants: A thermodynamic imperative
Authors: Hernandez Candia Carmen Noemi , Michaelian Karo 
Published: December 2010

AbstractIn this article we determine the importance of transpiration in plants at three different levels of organization: the plant itself, the ecosystem, and the biosphere, all in terms of entropy production due to transpiration. We propose that transpiration is a thermodynamic imperative rather than a physiological burden for the plant. Plants have not replaced their prevalent metabolism C3 with those of less loss of water, C4 or CAM. We argue that the fact that plants retain higher rates of transpiration along evolution could be explained using thermodynamic criteria.
ThePopulusClass III HD ZIP,popREVOLUTA, Influences Cambium Initiation and Patterning of Woody Stems
Authors: Robischon Marcel , Du Juan , Miura Eriko , Groover Andrew 
Published: December 2010

AbstractThe secondary growth of a woody stem requires the formation of a vascular cambium at an appropriate position and proper patterning of the vascular tissues derived from the cambium. Class III homeodomain-leucine zipper (HD ZIP) transcription factors have been implicated in polarity determination and patterning in lateral organs and primary vascular tissues and in the initiation and function of shoot apical meristems. We report here the functional characterization of a Populus class III HD ZIP gene, popREVOLUTA (PRE), that demonstrates another role for class III HD ZIPs in regulating the development of cambia and secondary vascular tissues. PRE is orthologous to Arabidopsis (Arabidopsis thaliana) REVOLUTA and is expressed in both the shoot apical meristem and in the cambial zone and secondary vascular tissues. Transgenic Populus expressing a microRNA-resistant form of PRE presents unstable phenotypic abnormalities affecting both primary and secondary growth. Surprisingly, phenotypic changes include abnormal formation of cambia within cortical parenchyma that can produce secondary vascular tissues in reverse polarity. Genes misexpressed in PRE mutants include transcription factors and auxin-related genes previously implicated in class III HD ZIP functions during primary growth. Together, these results suggest that PRE plays a fundamental role in the initiation of the cambium and in regulating the patterning of secondary vascular tissues.
Distribution of Callose Synthase, Cellulose Synthase, and Sucrose Synthase in Tobacco Pollen Tube Is Controlled in Dissimilar Ways by Actin Filaments and Microtubules
Authors: Cai Giampiero , Faleri Claudia , Del Casino Cecilia , Emons Anne Mie C. , Cresti Mauro 
Published: December 2010

Abstract
Callose and cellulose are fundamental components of the cell wall of pollen tubes and are probably synthesized by distinct enzymes, callose synthase and cellulose synthase, respectively. We examined the distribution of callose synthase and cellulose synthase in tobacco (Nicotiana tabacum) pollen tubes in relation to the dynamics of actin filaments, microtubules, and the endomembrane system using specific antibodies to highly conserved peptide sequences. The role of the cytoskeleton and membrane flow was investigated using specific inhibitors (latrunculin B, 2,3-butanedione monoxime, taxol, oryzalin, and brefeldin A). Both enzymes are associated with the plasma membrane, but cellulose synthase is present along the entire length of pollen tubes (with a higher concentration at the apex) while callose synthase is located in the apex and in distal regions. In longer pollen tubes, callose synthase accumulates consistently around callose plugs, indicating its involvement in plug synthesis. Actin filaments and endomembrane dynamics are critical for the distribution of callose synthase and cellulose synthase, showing that enzymes are transported through Golgi bodies and/or vesicles moving along actin filaments. Conversely, microtubules appear to be critical in the positioning of callose synthase in distal regions and around callose plugs. In contrast, cellulose synthases are only partially coaligned with cortical microtubules and unrelated to callose plugs. Callose synthase also comigrates with tubulin by Blue Native-polyacrylamide gel electrophoresis. Membrane sucrose synthase, which expectedly provides UDP-glucose to callose synthase and cellulose synthase, binds to actin filaments depending on sucrose concentration; its distribution is dependent on the actin cytoskeleton and the endomembrane system but not on microtubules.
Meta‐analysis of results from quantitative trait loci mapping studies on pig chromosome 4
Authors: Silva K. M. , Bastiaansen J. W. M. , Knol E. F. , Merks J. W. M. , Lopes P. S. , Guimarães S. E. F. , van Arendonk J. A. M. 
Published: December 2010

SummaryMeta‐analysis of results from multiple studies could lead to more precise quantitative trait loci (QTL) position estimates compared to the individual experiments. As the raw data from many different studies are not readily available, the use of results from published articles may be helpful. In this study, we performed a meta‐analysis of QTL on chromosome 4 in pig, using data from 25 separate experiments. First, a meta‐analysis was performed for individual traits: average daily gain and backfat thickness. Second, a meta‐analysis was performed for the QTL of three traits affecting loin yield: loin eye area, carcass length and loin meat weight. Third, 78 QTL were selected from 20 traits that could be assigned to one of three broad categories: carcass, fatness or growth traits. For each analysis, the number of identified meta‐QTL was smaller than the number of initial QTL. The reduction in the number of QTL ranged from 71% to 86% compared to the total number before the meta‐analysis. In addition, the meta‐analysis reduced the QTL confidence intervals by as much as 85% compared to individual QTL estimates. The reduction in the confidence interval was greater when a large number of independent QTL was included in the meta‐analysis. Meta‐QTL related to growth and fatness were found in the same region as the FAT1 region. Results indicate that the meta‐analysis is an efficient strategy to estimate the number and refine the positions of QTL when QTL estimates are available from multiple populations and experiments. This strategy can be used to better target further studies such as the selection of candidate genes related to trait variation.
Exploration of relationships between production and fertility traits in dairy cattle via association studies of SNPs within candidate genes derived by expression profiling
Authors: Pimentel E. C. G. , Bauersachs S. , Tietze M. , Simianer H. , Tetens J. , Thaller G. , Reinhardt F. , Wolf E. , König S. 
Published: December 2010

SummaryThe objective of this work was to integrate findings from functional genomics studies with genome‐wide association studies for fertility and production traits in dairy cattle. Association analyses of production and fertility traits with SNPs located within or close to 170 candidate genes derived from two gene expression studies and from the literature were performed. Data from 2294 Holstein bulls genotyped for 39557 SNPs were used. A total of 111 SNPs were located on chromosomal segments covered by a candidate gene. Allele substitution effects for each SNP were estimated using a mixed model with a fixed effect of marker and a random polygenic effect. Assumed covariance was derived either from marker or from pedigree information. Results from the analysis with the kinship matrix built from marker genotypes were more conservative than from the analysis with the pedigree‐derived relationship matrix. From sixteen SNPs with significant effects on both classes of traits, ten provided evidence of an antagonistic relationship between productivity and fertility. However, we found four SNPs with favourable effects on fertility and on yield traits, one SNP with favourable effects on fertility and percentage traits, and one SNP with antagonistic effects on two fertility traits. While most quantitative genetic studies have proven genetic antagonisms between yield and functional traits, improvements in both production and functionality may be possible when focusing on a few relevant SNPs. Investigations combining input from quantitative genetics and functional genomics with association analysis may be applied for the identification of such SNPs.
Inverse relationship between leukaemic cell burden and plasma concentrations of daunorubicin in patients with acute myeloid leukaemia
Authors: Bogason Alex , Quartino Angelica L. , Lafolie Pierre , Masquelier Michèle , Karlsson Mats O. , Paul Christer , Gruber Astrid , Vitols Sigurd 
Published: December 2010

WHAT IS ALREADY KNOWN ABOUT THE SUBJECT • In vitro studies show that daunorubicin (DNR) cytotoxicity decreases with increasing cell density because of a high cellular uptake and depletion of drug in the medium.• It is not known whether such an effect also occurs in vivo. WHAT THIS STUDY ADDS • We have shown that a large leukaemic cell burden lowers the plasma concentration of DNR in patients with acute myeloid leukaemia.• Our analysis supports that a large leukaemic cell burden increases the central volume of distribution for DNR.• Our study indicates that a dose adjustment of DNR may be of importance in acute myeloid leukaemia patients with high white blood cell counts.AIMS It has been shown that the cellular uptake and cytotoxicity of anthracyclines decrease with increasing cell density in vitro, an event termed ‘the inocculum effect’. It is not known whether such an effect occurs in vivo. In this study the relationships between white blood cell (WBC) count, plasma and cellular concentrations of daunorubicin (DNR) in patients with acute myeloid leukaemia were investigated.METHODS Plasma and mononuclear blood cells were isolated from peripheral blood from 40 patients with acute myeloid leukaemia at end of infusion (time 1 h), 5 and 24 h following the first DNR infusion. DNR concentrations were determined by high‐pressure liquid chromatography and related to the WBC count at diagnosis. A population pharmacokinetic model was used to estimate the correlations between baseline WBC count, volume of distribution and clearance of DNR.RESULTS A clear but weak inverse relationship between the baseline WBC count and plasma concentrations of DNR (r2 = 0.11, P < 0.05) at time 1 was found. Furthermore, a clear relationship between baseline WBC count and DNR central volume of distribution using population pharmacokinetic modelling (dOFV 4.77, P < 0.05) was also noted. Analysis of plasma DNR and the metabolite daunorubicinol (DOL) concentrations in patients with a high WBC count support that the low DNR/DOL concentrations are due a distribution effect.CONCLUSION This study shows that the leukaemic cell burden influences the plasma concentrations of anthracyclines. Further studies are needed to explore if patients with high a WBC count may require higher doses of anthracyclines.
Health‐related quality of life in pediatric liver transplant recipients compared with other chronic disease groups
Authors: Limbers Christine A. , Neighbors Katie , Martz Karen , Bucuvalas John C. , Webb Thomas , Varni James W. , Alonso Estella M. ,   on behalf of the Studies of Pediatric Liver Transplantation (SPLIT) Functional Outcomes Group (FOG)
Published: December 2010

Limbers CA, Neighbors K, Martz K, Bucuvalas JC, Webb T, Varni JW, Alonso EM, on behalf of the Studies of Pediatric Liver Transplantation (SPLIT) Functional Outcomes Group (FOG). Health‐related quality of life in pediatric liver transplant recipients compared with other chronic disease groups. Pediatr Transplantation 2011: 15: 245–253. © 2010 John Wiley & Sons A/S.Abstract:  This cross‐sectional, multicenter cohort study compares the level of HRQOL of pediatric LT recipients to children with other chronic health conditions. LT sample included 873 children who survived at least 12 months following LT. Six chronic disease samples were compiled from numerous studies, including over 800 patients with JRA, type 1 diabetes, cancer in remission, cardiac disease, end‐stage renal disease, and inflammatory bowel disease. Generic HRQOL was measured from both the parental and patient perspective using the PedsQL™ 4.0 Generic Core Scales. Pediatric LT patients reported better physical health than children with JRA. According to parents, pediatric LT recipients had better HRQOL than children on renal dialysis on all domains except school functioning. Across all domains but emotional functioning, pediatric LT recipients reported significantly lower HRQOL than children with type 1 diabetes. Overall, pediatric LT patients reported HRQOL comparable to that of children who had undergone renal transplantation and patients with cancer in remission. Pediatric LT patients manifested impaired HRQOL similar to that of children with chronic diseases and these data suggest that they face ongoing challenges that warrant monitoring and indicate a need for interventions to improve their HRQOL.
Japanese Guidelines for the Management of Respiratory Infectious Diseases in Children 2007 with focus on pneumonia
Authors: Uehara Suzuko , Sunakawa Keisuke , Eguchi Hiroyuki , Ouchi Kazunobu , Okada Kenji , Kurosaki Tomomichi , Suzuki Hiroshi , Tsutsumi Hiroyuki , Haruta Tsunekazu , Mitsuda Toshihiro , Yamazaki Tsutomu 
Published: December 2010

AbstractMembers of the Japanese Society of Pediatric Pulmonology and the Japanese Society for Pediatric Infectious Diseases developed the Guidelines for the Management of Respiratory Infectious Diseases in Children with the objective of facilitating the appropriate diagnosis and treatment of childhood respiratory infections. To date, a first edition (2004) and a revised edition (2007) have been issued. Many problems complicate the diagnosis of the pathogens responsible for bronchopulmonary infections in children. The Guidelines were the first pediatric guidelines in the world to recommend treatment with antimicrobials suited to causative pathogens as identified from cultures of sputum and other clinical specimens collected from infection sites and satisfying assessment criteria. The major causative microorganisms for pneumonia in infants and children were revealed to be Streptococcus pneumoniae, Haemophilus influenzae and Mycoplasma pneumoniae. This manuscript describes the Guidelines for the Management of Respiratory Infectious Diseases in Children in Japan 2007, with a focus on pneumonia.
Sleep characteristics of young children in Japan: Internet study and comparison with other Asian countries
Authors: Kohyama Jun , Mindell Jodi A. , Sadeh Avi 
Published: December 2010

AbstractBackground:  A recent international Internet‐based study of young children (birth to 36 months) found that total sleep duration in Japan was the shortest among 17 countries/regions. The present study compared features of children's sleep in Japan relative to those in other Asian countries/regions.Methods:  Parents of 872 infants and toddlers in Japan (48.6% boys), and parents of 20 455 infants and toddlers in 11 other Asian countries/regions (48.1% boys; China, Hong Kong, India, Indonesia, Korea, Malaysia, Philippines, Singapore, Taiwan, Thailand, and Vietnam) completed an Internet‐based expanded version of the Brief Infant Sleep Questionnaire.Results:  Young children in Japan exhibited significantly fewer nocturnal wakings and shorter daytime sleep in comparison with other Asian countries/regions. Although the former finding was apparent in all age groups, the reduced duration of daytime sleep in Japan was not present until after 3 months of age. Interestingly, sleep problems were reported by significantly fewer parents in Japan compared with those in other Asian countries/regions, although parents in Japan reported significantly more difficulty at bedtime.Conclusions:  The short sleep duration of young children in Japan is largely due to a relatively short duration of daytime sleep. Significant differences in sleep characteristics in Japan relative to other Asian regions were found primarily after 3 months of age. Future studies should further explore the underlying causes and the potential impacts of these sleep differences.
Nail psoriasis: a retrospective study on the effectiveness of systemic treatments (classical and biological therapy)
Authors: Sánchez‐Regaña M , Sola‐Ortigosa J , Alsina‐Gibert M , Vidal‐Fernández M , Umbert‐Millet P 
Published: December 2010

AbstractBackground  Nail psoriasis represents a challenge for specialists. There is no comparative study of systemic treatment’s effectiveness at this site. Objective: Evaluate the response of nail psoriasis to classical and biological therapy and to compare the effectiveness and safety of the different treatments.Methods  We performed a retrospective study of 84 patients with moderate–severe psoriasis seen at our Department between January 2006 and January 2009.Results  Psoriasis was severe in 53.4%. In 75% of cases, the fingernails were affected, and the mixed form was the most frequently subtype. The mean baseline scores on the PASI and the NAPSI were 23.12 and 14.7 respectively; the correlation between the two scores fell at weeks 12 and 24 but had risen again at week 48. The baseline NAPSI score tended to be lower in women and significantly higher in patients over 65 years of age, family history of psoriasis, severe psoriasis and nail matrix involvement. In our series, 58.3% received classical treatment (acitretin, methotrexate, cyclosporin, PUVA, NUVB, REPUVA, RENUVB) and 41.7% received biological treatment (infliximab, efalizumab, etanercept, adalimumab).Significant reductions were found (P < 0.05) in the mean NAPSI scores at 12, 24 and 48 weeks with all the antipsoriatic agents except NUVB; significantly greater with cyclosporine (P < 0.01) and biological as infliximab and adalimumab at 12 and 24 weeks (differences between treatments disappeared at 48 weeks).Conclusion  The response to treatment is slower in the nail lesions than in the skin lesions. The improvement of nail psoriasis is significant both with the classical treatments significantly higher in cyclosporin; and biological treatment (infliximab and adalimumab at 12 and 24 weeks).
Fenamates as TRP channel blockers: mefenamic acid selectively blocks TRPM3
Authors: Klose Chihab , Straub Isabelle , Riehle Marc , Ranta Felicia , Krautwurst Dietmar , Ullrich Susanne , Meyerhof Wolfgang , Harteneck Christian 
Published: December 2010

BACKGROUND AND PURPOSEFenamates are N‐phenyl‐substituted anthranilic acid derivatives clinically used as non‐steroid anti‐inflammatory drugs in pain treatment. Reports describing fenamates as tools to interfere with cellular volume regulation attracted our attention based on our interest in the role of the volume‐modulated transient receptor potential (TRP) channels TRPM3 and TRPV4.EXPERIMENTAL APPROACHFirstly, we measured the blocking potencies and selectivities of fenamates on TRPM3 and TRPV4 as well as TRPC6 and TRPM2 by Ca2+imaging in the heterologous HEK293 cell system. Secondly, we further investigated the effects of mefenamic acid on cytosolic Ca2+and on the membrane voltage in single HEK293 cells that exogenously express TRPM3. Thirdly, in insulin‐secreting INS‐1E cells, which endogenously express TRPM3, we validated the effect of mefenamic acid on cytosolic Ca2+and insulin secretion.KEY RESULTSWe identified and characterized mefenamic acid as a selective and potent TRPM3 blocker, whereas other fenamate structures non‐selectively blocked TRPM3, TRPV4, TRPC6 and TRPM2.CONCLUSIONS AND IMPLICATIONSThis study reveals that mefenamic acid selectively inhibits TRPM3‐mediated calcium entry. This selectivity was further confirmed using insulin‐secreting cells. KATPchannel‐dependent increases in cytosolic Ca2+and insulin secretion were not blocked by mefenamic acid, but the selective stimulation of TRPM3‐dependent Ca2+entry and insulin secretion induced by pregnenolone sulphate were inhibited. However, the physiological regulator of TRPM3 in insulin‐secreting cells remains to be elucidated, as well as the conditions under which the inhibition of TRPM3 can impair pancreatic β‐cell function. Our results strongly suggest mefenamic acid is the most selective fenamate to interfere with TRPM3 function.