bluuebunny/crossref_metadata_2025_split huggingface dataset:
Papiloma oral de células escamosas na papila interincisiva: Revisão da literatura em um relato de caso
Authors: Heras Olalla Geovanna Sofía , Zhunio Cárdenas Anthony Stalin , Alvarado Cordero Jacinto José 
Published: May 2024

El papiloma oral de células escamosas es una proliferación exofítica localizada, de apariencia verrugosa o similar a una coliflor, asintomática y de crecimiento lento. Es la lesión benigna más común del epitelio oral, no tiene predilección por el sexo y comúnmente se presenta entre la tercera y quinta décadas de vida, aunque en ocasiones aparece en niños; frecuentemente se ubica en el paladar, lengua, labios o encías. El tratamiento de elección es la extirpación quirúrgica total de la lesión, no obstante, existen otras modalidades de tratamiento, la lesión no suele ser recurrente y el riesgo de transformarse en una lesión maligna es bajo. El objetivo de este artículo es analizar los puntos claves como características clínicas e histopatológicas, diagnóstico, tratamiento y recurrencia, y presentar un caso clínico de un papiloma oral de células escamosas ubicado en la papila interincisiva.
Utilização de enxertos ósseos na reabilitação oral com implantes
Authors: Silva Giulia Crislane de Sousa e , Maia Yasmin Caroline de Sousa , Lopes Matheus Araújo Brito Santos , Campos Marta Rosado de Oliveira , Veras Eduardo Souza de Lobão 
Published: May 2024

Introdução A perda óssea ocorre após extração dentária, fisiologicamente pelo envelhecimento, após acidentes, periodontites ou, ainda, pelo uso de próteses móveis apoiadas no rebordo edêntulo. Esse fenômeno que contribui de alguma forma para limitar a reabilitação com implantes osseointegrados. Assim, se faz necessária a realização de técnicas de enxertia óssea. Objetivo: analisar a utilização de enxertos ósseos em reabilitações orais com implantes. Metodologia: A referida pesquisa é uma revisão literária integrativa. A fundamentação científica foi efetuada nas seguintes bases de dados: Biblioteca Virtual em Saúde, Scientific Electronic Library Online e PubMed, empregando expressões de busca como implantes dentários, enxertos ósseos, reabsorção óssea, implante dentário osseointegrado, cobrindo o período de 2007 a 2023. Resultados: De acordo com os critérios de elegibilidade 20 artigos foram encontrados, após triagem da literatura, 14 artigos foram inclusos e tiveram dados tabulados. Conclusão: Conclui-se sua eficácia através de estudos na reabilitação com implantes dentários e a correta indicação do tipo de enxerto a ser usado é um fator essencial ao processo de regeneração óssea para o sucesso do procedimento.
UPAYA MENINGKATKAN HASIL BELAJAR MATEMATIKA PADA MATERI PERKALIAN DENGAN METODE JARIMATIKA SISWA KELAS IV
Authors: Hanifa Rosda , Syehma Bahtiar Reza 
Published: May 2024

Kesulitan siswa dalam mempelajari dan menyelesaikan soal materi perkalian dipengaruhi oleh beberapa faktor satu diantaranya adalah metode yang dipakai guru harus tepat dan menyenangkan. Untuk meningkatkan hasil belajar matematika pada materi perkalian di kelas IV SDN Pakis V Surabaya, menekankan kepada penggunaan metode untuk mempermudah siswa dalam menyelesaikan soal-soal perkalian. Penggunaan metode yang dapat memudahkan siswa dan mudah ditemukan alat konkretnya nampaknya harus diterapkan, hal ini dimaksudkan agar tujuan pembelajaran matematika yang sudah ditetapkan kurikulum dapat tercapai dan sesuai dengan perkembangan kognitif siswa. Salah satu metode yang diharapkan dapat meningkatkan kemampuan berhitung serta membangkitkan semangat untuk belajar karena metodenya merupakan kegiatan yang menyenangkan, metode yang digunakan adalah metode jarimatika yang menggunakan 10 jari sebagai alat bantu untuk proses berhitung perkalian. Melalui metode jarimatika, siswa tidak perlu membeli alat peraga karena metode ini sangat praktis dengan menggunakan jari-jari tangan sebagai alat berhitung, sedangkan jari tangan dimiliki oleh setiap siswa dan memenuhi tahap perkembangan kognitifnya, karena siswa akan melakukan sesuatu yang berkaitan dengan konsep yang berkaitan yaitu perkalian.
Kata Kunci : Matematika, jarimatika, perkalian, dan hasil belajar.
Decreased but persistent epigenetic age acceleration is associated with changes in T-cell subsets after initiation of highly active antiretroviral therapy in persons living with HIV
Authors: Sehl Mary E. , Breen Elizabeth Crabb , Shih Roger , Li Fengxue , Zhang Joshua , Langfelder Peter , Horvath Steve , Bream Jay H. , Duggal Priya , Martinson Jeremy , Wolinsky Steven M. , Martinez-Maza Otoniel , Ramirez Christina M. , Jamieson Beth D. 
Published: May 2024

IntroductionPersons living with HIV (PLWH) experience the early onset of age-related illnesses, even in the setting of successful human immunodeficiency virus (HIV) suppression with highly active antiretroviral therapy (HAART). HIV infection is associated with accelerated epigenetic aging as measured using DNA methylation (DNAm)-based estimates of biological age and of telomere length (TL).MethodsDNAm levels (Infinium MethylationEPIC BeadChip) from peripheral blood mononuclear cells from 200 PLWH and 199 HIV-seronegative (SN) participants matched on chronologic age, hepatitis C virus, and time intervals were used to calculate epigenetic age acceleration, expressed as age-adjusted acceleration residuals from 4 epigenetic clocks [Horvath’s pan-tissue age acceleration residual (AAR), extrinsic epigenetic age acceleration (EEAA), phenotypic epigenetic age acceleration (PEAA), and grim epigenetic age acceleration (GEAA)] plus age-adjusted DNAm-based TL (aaDNAmTL). Epigenetic age acceleration was compared for PLWH and SN participants at two visits: up to 1.5 years prior and 2–3 years after HAART (or equivalent visits). Flow cytometry was performed in PLWH and SN participants at both visits to evaluate T-cell subsets.ResultsEpigenetic age acceleration in PLWH decreased after the initiation of HAART but remained greater post-HAART than that in age-matched SN participants, with differences in medians of 6.6, 9.1, and 7.7 years for AAR, EEAA, and PEAA, respectively, and 0.39 units of aaDNAmTL shortening (all p &lt; 0.001). Cumulative HIV viral load after HAART initiation was associated with some epigenetic acceleration (EEAA, PEAA, and aaDNAmTL), but even PLWH with undetectable HIV post-HAART showed persistent epigenetic age acceleration compared to SN participants (p &lt; 0.001). AAR, EEAA, and aaDNAmTL showed significant associations with total, naïve, and senescent CD8 T-cell counts; the total CD4 T-cell counts were associated with AAR, EEAA, and PEAA (p = 0.04 to &lt;0.001). In an epigenome-wide analysis using weighted gene co-methylation network analyses, 11 modules demonstrated significant DNAm differences pre- to post-HAART initiation. Of these, nine were previously identified as significantly different from pre- to post-HIV infection but in the opposite direction.DiscussionIn this large longitudinal study, we demonstrated that, although the magnitude of the difference decreases with HAART is associated with the cumulative viral load, PLWH are persistently epigenetically older than age-matched SN participants even after the successful initiation of HAART, and these changes are associated with changes in T-cell subsets.
Research on the cost allocation method of deep sea wind power considering carbon trading and green certificate trading
Authors: Xue Guiyuan , Niu Wenjuan , Chen Chen , Wu Yin , Zhu Xiaojun 
Published: May 2024

Climate change imposes tighter limits on carbon emissions, which require the development of more green electricity. Deep sea wind power has the advantages of high wind energy density, high power generation utilization hours, no land occupation, and near to power load centers for easy on-site consumption, which lead to a broad market prospect. In the foreseeable future, deep sea wind power will usher in large-scale construction and development. The initial investment and construction cost of deep sea wind power is high, subsidies are gradually decreasing, and there is a lack of full life cycle economic analysis combined with various market policies, resulting in a lack of guidance for its investment and construction. In order to promote the development of deep sea wind power, relevant cost recovery mechanisms must be found. This paper proposes a system cost allocation method based on the comparative difference method and load similarity with consideration of the transformation and operation costs of thermal power, demand-side response, and energy storage; a cost allocation method based on carbon trading and green certificate trading income is proposed; and a time sequence optimization operation simulation model is established to maximize the deep sea wind power income. A case study is proposed for a 300 MW deep sea wind farm, the results show that the cost allocation method and cost allocation model proposed in this paper have a significant effect on wind power cost recovery, and the effectiveness of the proposed method and model is verified.
Absence of causative genetic association between Helicobacter pylori infection and glaucoma: a bidirectional two-sample mendelian randomization study
Authors: Zhang Yan , Huang Yihong , Wu Yuyu , Zhang Jinying , Chen Wanzhu , Xu Danfeng , Guo Maosheng 
Published: May 2024

Background: While clinical research has indicated a potential link between Helicobacter pylori infection and the onset of glaucoma, the causality of this association remains uncertain due to the susceptibility of observational studies to confounding factors and reverse causation.Methods: A comprehensive two-sample bidirectional Mendelian randomization (MR) analysis was conducted to assess the causal connection between H. pylori infection and glaucoma. Glaucoma was categorized into primary open-angle glaucoma (POAG), normal tension glaucoma (NTG), and pseudo-exfoliation glaucoma (PEG). Various methods, including inverse variance weighted, MR-Egger regression, weighted median, and mode-based estimator, were employed for effect estimation and pleiotropy testing. To enhance result robustness, a sensitivity analysis was performed by excluding proxy single nucleotide polymorphisms.Results: Genetic predisposition for H. pylori infection has no causal effect on glaucoma: (OR 1.00; 95% CI 0.95–1.06, p = 0.980), (OR 0.97; 95% CI 0.86–1.09, p = 0.550), and (OR 0.99; 95% CI 0.90–1.08, p = 0.766) with POAG, NTG, and PEG, respectively. An inverse MR showed no causal effect of POAG, NTG, and PEG on H. pylori infection (OR 1.01; 95% CI 0.97–1.05, p = 0.693), (OR 1.00; 95% CI 0.98–1.03, p = 0.804), and (OR 0.99; 95% CI 0.96–1.01, p = 0.363), respectively. Heterogeneity (p &gt; 0.05) and pleiotropy (p &gt; 0.05) analysis confirmed the robustness of MR results.Conclusion: These results indicated that there was no genetic evidence for a causal link between H. pylori and glaucoma, suggesting that the eradication or prevention of H. pylori infection might not benefit glaucoma and vice versa.
Oral curcumin ameliorates acute murine campylobacteriosis
Authors: Heimesaat Markus M. , Mousavi Soraya , Lobo de Sá Fábia Daniela , Peh Elisa , Schulzke Jörg-Dieter , Bücker Roland , Kittler Sophie , Bereswill Stefan 
Published: May 2024

IntroductionHuman infections with the food-borne enteropathogen Campylobacter jejuni are responsible for increasing incidences of acute campylobacteriosis cases worldwide. Since antibiotic treatment is usually not indicated and the severity of the enteritis directly correlates with the risk of developing serious autoimmune disease later-on, novel antibiotics-independent intervention strategies with non-toxic compounds to ameliorate and even prevent campylobacteriosis are utmost wanted. Given its known pleiotropic health-promoting properties, curcumin constitutes such a promising candidate molecule. In our actual preclinical placebo-controlled intervention trial, we tested the anti-microbial and anti-inflammatory effects of oral curcumin pretreatment during acute experimental campylobacteriosis.MethodsTherefore, secondary abiotic IL-10-/- mice were challenged with synthetic curcumin via the drinking water starting a week prior oral C. jejuni infection. To assess anti-pathogenic, clinical, immune-modulatory, and functional effects of curcumin prophylaxis, gastrointestinal C. jejuni bacteria were cultured, clinical signs and colonic histopathological changes quantitated, pro-inflammatory immune cell responses determined by in situ immunohistochemistry and intestinal, extra-intestinal and systemic pro-inflammatory mediator measurements, and finally, intestinal epithelial barrier function tested by electrophysiological resistance analysis of colonic ex vivo biopsies in the Ussing chamber.Results and discussionWhereas placebo counterparts were suffering from severe enterocolitis characterized by wasting symptoms and bloody diarrhea on day 6 post-infection, curcumin pretreated mice, however, were clinically far less compromised and displayed less severe microscopic inflammatory sequelae such as histopathological changes and epithelial cell apoptosis in the colon. In addition, curcumin pretreatment could mitigate pro-inflammatory innate and adaptive immune responses in the intestinal tract and importantly, rescue colonic epithelial barrier integrity upon C. jejuni infection. Remarkably, the disease-mitigating effects of exogenous curcumin was also observed in organs beyond the infected intestines and strikingly, even systemically given basal hepatic, renal, and serum concentrations of pro-inflammatory mediators measured in curcumin pretreated mice on day 6 post-infection. In conclusion, the anti-Campylobacter and disease-mitigating including anti-inflammatory effects upon oral curcumin application observed here highlight the polyphenolic compound as a promising antibiotics-independent option for the prevention from severe acute campylobacteriosis and its potential post-infectious complications.
Determining diagnostic delays in Romanian multiple myeloma patients using the Aarhus statement
Authors: Irimia Ruxandra , Badelita Sorina , Barbu Sinziana , Cirlan Ioana Loredana , Zidaru Larisa , Coriu Daniel 
Published: May 2024

IntroductionMultiple Myeloma (MM) is classified as one of the most challenging cancers to diagnose, and the hematological malignancy is associated with prolonged diagnostic delays. Although major steps have been made in the improvement of MM patient diagnosis and care, Romanian patients still face long diagnostic delays. Thus far, there have been no studies evaluating the factors associated with diagnostic errors in Romanian MM patients.MethodsUsing the Aarhus statement, we prospectively determined the diagnostic intervals for 103 patients diagnosed with MM at Fundeni Clinical Institute, between January 2022 and March 2023.ResultsOur data revealed that the main diagnostic delays are experienced during the “patient interval.” Patients spend a median of 162 days from the first symptom onset until the first doctor appointment. Bone pain is the most frequently reported symptom by patients (78.64%), but it leads to a medical-seeking behavior in only half of the reporting patients and results in a median delay of 191 days. The changes in routine lab tests are considered most worrisome for patients, leading to a medical appointment after a median of only 25 days. The median primary care interval was 70 days, with patients having an average of 3.7 medical visits until MM suspicion was first raised. The secondary care interval did not contribute to the diagnostic delays.DiscussionOverall, the median diagnostic path for MM patients in Romania was more than 6 months, leading to a higher number of emergency presentations and myeloma-related end-organ damage.
Estuarine mangrove niches select cultivable heterotrophic diazotrophs with diverse metabolic potentials—a prospective cross-dialog for functional diazotrophy
Authors: Mondal Sumana , Biswas Biswajit , Chowdhury Rajojit , Sengupta Rudranil , Mandal Anup , Kotal Hemendra Nath , Giri Chayan Kumar , Ghosh Anjali , Saha Subhajit , Begam Mst Momtaj , Mukherjee Chandan , Das Ipsita , Basak Sandip Kumar , Mitra Ghosh Mahashweta , Ray Krishna 
Published: May 2024

IntroductionBiological nitrogen fixation (BNF), an unparalleled metabolic novelty among living microorganisms on earth, globally contributes ~88-101 Tg N year−1 to natural ecosystems, ~56% sourced from symbiotic BNF while ~22-45% derived from free-living nitrogen fixers (FLNF). The success of symbiotic BNF is largely dependent on its interaction with host-plant, however ubiquitous environmental heterotrophic FLNFs face many limitations in their immediate ecological niches to sustain unhindered BNF. The autotrophic FLNFs like cyanobacteria and oceanic heterotrophic diazotrophs have been well studied about their contrivances acclimated/adapted by these organisms to outwit the environmental constraints for functional diazotrophy. However, FLNF heterotrophs face more adversity in executing BNF under stressful estuarine/marine/aquatic habitats.MethodsIn this study a large-scale cultivation-dependent investigation was accomplished with 190 NCBI accessioned and 45 non-accessioned heterotrophic FLNF cultivable bacterial isolates (total 235) from halophilic estuarine intertidal mangrove niches of Indian Sundarbans, a Ramsar site and UNESCO proclaimed World Heritage Site. Assuming ~1% culturability of the microbial community, the respective niches were also studied for representing actual bacterial diversity via cultivation-independent next-generation sequencing of V3-V4 rRNA regions.ResultsBoth the studies revealed a higher abundance of culturable Gammaproteobacteria followed by Firmicutes, the majority of 235 FLNFs studied belonging to these two classes. The FLNFs displayed comparable selection potential in media for free nitrogen fixers and iron-oxidizing bacteria, linking diazotrophy with iron oxidation, siderophore production, phosphorus solubilization, phosphorus uptake and accumulation as well as denitrification.DiscussionThis observation validated the hypothesis that under extreme estuarine mangrove niches, diazotrophs are naturally selected as a specialized multidimensional entity, to expedite BNF and survive. Earlier metagenome data from mangrove niches demonstrated a microbial metabolic coupling among C, N, P, S, and Fe cycling in mangrove sediments, as an adaptive trait, evident with the co-abundant respective functional genes, which corroborates our findings in cultivation mode for multiple interrelated metabolic potential facilitating BNF in a challenging intertidal mangrove environment.
Association between gut microbiota and common overlapping gastrointestinal disorders: a bidirectional two-sample Mendelian randomization study
Authors: Huang Yuhan , Kang Zhen , He Yuhan , Qiu Yi , Song Yuhui , Liu Weiai 
Published: May 2024

BackgroundThe main functional gastrointestinal disorders (FGIDs) include functional dyspepsia (FD) and irritable bowel syndrome (IBS), which often present overlapping symptoms with gastroesophageal reflux disease (GERD), posing a challenge for clinical diagnosis and treatment. The gut microbiota is closely associated with FGIDs and GERD, although the causal relationship has not been fully elucidated. Therefore, we aimed to investigate the potential causal relationship using bidirectional two-sample Mendelian randomization (MR) analysis.Materials and methodsThe genetic data of the 211 gut microbiota were obtained from the MiBioGen consortium (N = 14,306, from phylum to genus level) and species level of gut microbiota were acquired from the Dutch Microbiome Project (N = 7,738). For FD and IBS, we utilized the FinnGen consortium, whereas, for GERD data analysis, we obtained the IEU OpenGWAS project. The inverse-variance weighted (IVW) method was used as the primary method to calculate causal effect values. Sensitivity analyses were also performed to confirm the robustness of the primary findings of the MR analyses. Moreover, a reverse MR analysis was conducted to assess the likelihood of reverse causality.ResultsCombining the results of the preliminary and sensitivity analyses, we identified that 8 gut microbial taxa were associated with FD. Genus Lachnospiraceae NK4A136 group (p = 3.63 × 10−3) and genus Terrisporobacter (p = 1.13 × 10−3) were strongly associated with FD. At the same time, we found that 8 gut microbial taxa were associated with IBS. Family Prevotellaceae (p = 2.44 × 10−3) and species Clostridium leptum (p = 7.68 × 10−3) display a robust correlation with IBS. In addition, 5 gut microbial taxa were associated with GERD using the IVW approach. In the reverse MR analysis, 2 gut microbial taxa were found to be associated with FD, 5 gut microbial taxa were found to be associated with IBS, and 21 gut microbial taxa were found to be associated with GERD.ConclusionThe study reveals the potential causal effects of specific microbial taxa on FD, IBS, and GERD and may offer novel insights into the diagnosis and treatment of these conditions.
The role of digestive system diseases in cerebrovascular disease: a comprehensive Mendelian randomization study
Authors: Qin Hao , Suo Shihuan , Yang Fan , Hao Pengfei , Zhang Xianfeng 
Published: May 2024

BackgroundCerebrovascular disease, among the most prevalent neurological disorders, poses a substantial threat to human health with its elevated mortality and disability rates, placing considerable strain on healthcare systems. Although several studies in recent years have suggested a potential association between digestive system diseases and cerebrovascular diseases, the findings remain inconsistent.MethodsGenome-wide association study (GWAS) summary data for 12 digestive diseases and cerebrovascular diseases were used to conduct Mendelian randomization (MR) analysis. In this investigation, we endeavored to elucidate the causal relationship between digestive system diseases and cerebrovascular diseases. Employing a comprehensive approach, including two-sample MR (TSMR), multivariate MR (MVMR), and two-step MR analysis, we leveraged summary statistics data obtained from published GWAS. The primary analysis method employed was inverse variance weighted (IVW), with MR-Egger and weighted median (WM) as secondary methods. Sensitivity analysis included heterogeneity testing, horizontal multivariate testing, MR-PRESSO, and a “leave-one-out” method. Additionally, the F-statistic was utilized to assess the strength of instrumental variables, ensuring robust results.ResultsIn the TSMR analysis, this study found a significant causal relationship between genetically predicted gastroesophageal reflux disease (GERD) and any stroke (AS), any ischemic stroke (AIS), large-artery atherosclerotic stroke (LAS), intracranial aneurysm (IA), and subarachnoid hemorrhage (SAH). In MVMR analysis, this study found that even after adjusting for systolic blood pressure (SBP), body mass index (BMI) and type 2 diabetes (T2D), the causal relationship remains exist. In the two-step MR mediation analysis, it was found that BMI, SBP and T2D play mediating role in the causal relationship between GERD and cerebrovascular diseases.ConclusionThis study indicates a clear positive causal relationship between GERD and cerebrovascular diseases, and this causal association remains significant even after adjusting for BMI, SBP and T2D. The mediation MR analysis suggests that BMI, SBP and T2D may mediate the causal relationship between GERD and the risk of cerebrovascular diseases.