bluuebunny/crossref_metadata_2025_split huggingface dataset:
Drug eluting stenting in bifurcation coronary lesions long‐term results applying a systematic treatment strategy
Authors: Assali Abid R. , Vaknin‐Assa Hana , Lev Eli , Teplitsky Igal , Dvir Danny , Brosh David , Bental Tamir , Battler Alexander , Kornowski Ran 
Published: December 2011

AbstractObjectives: To explore the long‐term results following implantation of drug‐eluting stents (DES) in bifurcation lesions according to contemporary “real world” practice. Background: Limited information is available on the long‐term outcomes of patients with bifurcation lesions who are treated using DES. A systematic approach for bifurcation lesion management was applied, using either a “provisional” single stent technique or a dedicated two stents strategy according to the side‐branch diameter and severity of its ostial stenosis. Methods: Four hundred one consecutive patients underwent bifurcation percutaneous coronary intervention (PCI) using DES and were included in our prospective registry. All adverse events were recorded up to 2 years and distinguished according to the planned PCI strategy (e.g., one versus two stents technique). Results: A planned two stents strategy was used in 141 patients (35% of patients). In 260 patients (65%), the planned treatment involved stenting of the main branch only with “provisional” stenting of the side‐branch according to procedural course. Thus, 24 patients (9.2%) needed additional stenting at the side‐branch to complete the PCI. Cumulative major adverse cardiac event rate at 1 and 2 years was similar for both groups (11.4% vs. 14.8% at 1 year and 19.4% vs. 25.7% at 2 years for the single vs. two stents groups, accordingly, P = NS for both). Likewise, there was no difference in mortality, cardiac mortality, myocardial infarction, need for target lesions or target vessel revascularization, or definite stent thrombosis rate between the two groups at 6, 12, and 2 years follow‐up. The rate of angiographically confirmed (i.e., definite) stent thrombosis did not differ between the two groups during follow‐up. Conclusions: Our study revealed favorable long‐term clinical results following DES implantation using a systematic, rather simplified approach towards bifurcation stenting and using either a single or double stenting technique. © 2011 Wiley Periodicals, Inc.
Superheated Atomization: Influence of Nozzle Geometry on Mass Flux and Spray Characteristics
Authors: Günther Astrid , Wirth Karl‐Ernst 
Published: December 2011

AbstractBei überhitzter Zerstäubung wird die Desintegration des Sprays durch die von thermischer Energie induzierte partielle Verdampfung verstärkt. Der Verdampfungsgrad und die Entstehung großer Einzeltropfen sind von der Düsengeometrie abhängig. Durch Ermittlung der Massenströme und mit optischen Messmethoden werden geometrieabhängige Veränderungen der Strömungscharakteristik und der Spraymorphologie untersucht.
Synthesis and Functional Characterization of Tridegin and Its Analogues: Inhibitors and Substrates of Factor XIIIa
Authors: Böhm Miriam , Kühl Toni , Hardes Kornelia , Coch Richard , Arkona Christoph , Schlott Bernhard , Steinmetzer Torsten , Imhof Diana 
Published: December 2011

AbstractTridegin, a 66‐mer peptide isolated from the leech Haementeria ghilianii, is a potent inhibitor of the coagulation factor XIIIa. This paper describes the chemical synthesis of tridegin by two different strategies—solid‐phase assembly and native chemical ligation—both followed by oxidation in solution phase. Tridegin and truncated analogues were examined for their activity and revealed a particular importance of the C‐terminal region of the parent peptide. Based on these studies a minimal sequence required for factor XIIIa inhibition could be identified. Our data revealed that the glutamine residue at position 52 (Q52) of tridegin most likely binds to the active site of factor XIIIa and was therefore suggested to react with the enzyme. The function of the N‐terminal region is also discussed, as the isolated C‐terminal segment of tridegin lost its inhibitory activity rapidly in the presence of factor XIIIa, whereas this was not the case for the full‐length inhibitor.
Chloroplast genome sequence confirms distinctness of Australian and Asian wild rice
Authors: Waters Daniel L. E. , Nock Catherine J. , Ishikawa Ryuji , Rice Nicole , Henry Robert J. 
Published: December 2011

AbstractCultivated rice (Oryza sativa) is an AA genome Oryza species that was most likely domesticated from wild populations of O. rufipogon in Asia. O. rufipogon and O. meridionalis are the only AA genome species found within Australia and occur as widespread populations across northern Australia. The chloroplast genome sequence of O. rufipogon from Asia and Australia and O. meridionalis and O. australiensis (an Australian member of the genus very distant from O. sativa) was obtained by massively parallel sequencing and compared with the chloroplast genome sequence of domesticated O. sativa. Oryza australiensis differed in more than 850 sites single nucleotide polymorphism or indel from each of the other samples. The other wild rice species had only around 100 differences relative to cultivated rice. The chloroplast genomes of Australian O. rufipogon and O. meridionalis were closely related with only 32 differences. The Asian O. rufipogon chloroplast genome (with only 68 differences) was closer to O. sativa than the Australian taxa (both with more than 100 differences). The chloroplast sequences emphasize the genetic distinctness of the Australian populations and their potential as a source of novel rice germplasm. The Australian O. rufipogon may be a perennial form of O. meridionalis.
Effects of typhoon Morakot on a large shallow lake ecosystem, Lake Taihu, China
Authors: Ding Yanqing , Qin Boqiang , Zhu Guangwei , Wu Tingfeng , Wang Yongping , Luo Liancong 
Published: December 2011

ABSTRACTA case study of typhoon‐induced sediment resuspension and its effects on some indicators of water quality (dissolved oxygen, pH and turbidity) and nutrient release in the large shallow eutrophic Lake Taihu, the third largest freshwater lake in China, was documented, and its effect on the algal biomass was investigated. Typhoon Morakot that swept from the west of Lake Taihu in August 2009 led to a number of changes in the lake ecosystem: (1) intensive winds produced large seiches, strong waves and large wave‐induced shear stress; (2) typhoon Morakot led to increased suspended solids and nutrients in the water column. Post‐typhoon concentrations of suspended solid, total phosphorus, total nitrogen, soluble reactive phosphorus (SRP). NO3‐N and NH4‐N were 4, 2, 1·5, 2·5, 3·5 and 1·5 times those observed in the pre‐typhoon water column, respectively; and (3) during typhoon Morakot, there was no obvious stratification between the surface and bottom of the water column in chlorophyll a (Chl a), the post‐typhoon Chl a concentration throughout the water column increased with a maximum daily mean of 87·8 µg l−1, and the algal bloom recurred. Typhoon‐induced sediment resuspension could lead to both nutrient and light limitations for algal growth, whereas after the typhoon had passed, water column conditions (water temperature, solar radiation, light penetration and nutrient availability) favoured the growth and production of algae. Copyright © 2011 John Wiley & Sons, Ltd.
Capillary electrophoresis of natural products: Highlights of the last five years (2006–2010)
Authors: Rabanes Heide R. , Guidote Armando M. , Quirino Joselito P. 
Published: December 2011

AbstractAn extensive search of the published research and review articles indicated a growing number of works that employed electroseparation techniques. Capillary zone electrophoresis (CZE) and electrokinetic chromatography (EKC), in particular, were found to be popular for the analyses of natural products. This review outlines the important developments in this field over the years, especially during the 2006–2010 period. An overview of the natural product applications such as pharmaceuticals/herbal products, fingerprinting and quality control, food and food contaminants, and toxicological compounds of interest to forensics were presented. Important areas of detection strategies, microchips, sample preconcentration, and chiral separations were also discussed. Hence, quick information was provided on the researches already undertaken and the possibilities of unexplored areas. In addition, fundamental concepts for the understanding of CZE and EKC and their suitability for natural products analyses were briefly discussed.
The Wnt secretion protein Evi/Gpr177 promotes glioma tumourigenesis
Authors: Augustin Iris , Goidts Violaine , Bongers Angelika , Kerr Grainne , Vollert Gordon , Radlwimmer Bernhard , Hartmann Christian , Herold‐Mende Christel , Reifenberger Guido , von Deimling Andreas , Boutros Michael 
Published: December 2011

AbstractMalignant astrocytomas are highly aggressive brain tumours with poor prognosis. While a number of structural genomic changes and dysregulation of signalling pathways in gliomas have been described, the identification of biomarkers and druggable targets remains an important task for novel diagnostic and therapeutic approaches. Here, we show that the Wnt‐specific secretory protein Evi (also known as GPR177/Wntless/Sprinter) is overexpressed in astrocytic gliomas. Evi/Wls is a core Wnt signalling component and a specific regulator of pan‐Wnt protein secretion, affecting both canonical and non‐canonical signalling. We demonstrate that its depletion in glioma and glioma‐derived stem‐like cells led to decreased cell proliferation and apoptosis. Furthermore, Evi/Wls silencing in glioma cells reduced cell migration and the capacity to form tumours in vivo. We further show that Evi/Wls overexpression is sufficient to promote downstream Wnt signalling. Taken together, our study identifies Evi/Wls as an essential regulator of glioma tumourigenesis, identifying a pathway‐specific protein trafficking factor as an oncogene and offering novel therapeutic options to interfere with the aberrant regulation of growth factors at the site of production.
Evidence for an increase in trisomy 21 (Down syndrome) in Europe after the Chernobyl reactor accident
Authors: Sperling Karl , Neitzel Heidemarie , Scherb Hagen 
Published: December 2011

AbstractThe objective of this study is to investigate the prevalence of Down syndrome (DS) associated with Chernobyl fallout. Maternal age‐adjusted DS data and corresponding live birth data from the following seven European countries or regions were analyzed: Bavaria and West Berlin in Germany, Belarus, Hungary, the Lothian Region of Scotland, North West England, and Sweden from 1981 to 1992. To assess the underlying time trends in the DS occurrence, and to investigate whether there have been significant changes in the trend functions after Chernobyl, we applied logistic regression allowing for peaks and jumps from January 1987 onward. The majority of the trisomy 21 cases of the previously reported, highly significant January 1987 clusters in Belarus and West Berlin were conceived when the radioactive clouds with significant amounts of radionuclides with short physical half‐lives, especially 131iodine, passed over these regions. Apart from this, we also observed a significant longer lasting effect in both areas. Moreover, evidence for long‐term changes in the DS prevalence in several other European regions is presented and explained by exposure, especially to 137Cs. In many areas, 137Cs uptake reached its maximum one year after the Chernobyl accident. Thus, the highest increase in trisomy 21 should be observed in 1987/1988, which is indeed the case. Based on the fact that maternal meiosis is an error prone process, the assumption of a causal relationship between low‐dose irradiation and nondisjunction is the most likely explanation for the observed increase in DS after the Chernobyl reactor accident. Genet. Epidemiol. 36 : 48–55, 2012. © 2011 Wiley Periodicals, Inc.
Isolation, characterization, and genetic profiling of subpopulations of olfactory ensheathing cells from the olfactory bulb
Authors: Honoré Axel , Le corre Stéphanie , Derambure Céline , Normand Romain , Duclos Célia , Boyer Olivier , Marie Jean‐Paul , Guérout Nicolas 
Published: December 2011

AbstractOlfactory ensheathing cells (OECs) play a crucial role during neurogenesis of primary olfactory neurons. Transplantation of OECs is considered as a promising new therapy for central nervous system repair. Nevertheless, OECs are constituted of distinct subpopulations and their role during neurogenesis is not clearly understood. In particular, OECs from the olfactory bulb (OB) constitute a heterogeneous, but not yet isolated and characterized, population of cells. In our study, flow cytometry analyses of primary OB cultures, based on cell surface expression of low‐affinity nerve growth factor receptor (p75), reveal the presence of two distinct populations of OECs. Indeed, some of them express a high level of p75 (P75High) and the other a low level of p75 (P75Low). Effects of OB microenvironment were assessed, and we were able to show that fibroblasts mediate the induction of these two populations through the secretion of soluble factors. To characterize P75High and P75Low OECs, cells were sorted based on their differential expression of p75. Microarray analyses revealed that P75High OECs overexpress genes implicated in modulation of extracellular matrix and cell sorting, whereas P75Low OECs overexpress genes involved in regulation of the inflammatory response and axonal guidance. These results permit, for the first time, to isolate the two distinct subpopulations of OECs from OB, and suggest their specific role during neurogenesis. © 2011 Wiley Periodicals, Inc.
Impact messages of depressed outpatients as perceived by their significant others: profiles, therapeutic change, and relationship to outcome
Authors: Grosse Holtforth Martin , Altenstein David , Ansell Emily , Schneider Claudia , Caspar Franz 
Published: December 2011

AbstractWhereas previous interpersonal research in depression has frequently used self‐reports, patients' impact on others is rarely analyzed. We analyzed impacts of 180 depressed psychotherapy outpatients out of 832 diagnostically heterogeneous patients as rated by their significant others. Depressed patients were perceived as more submissive, hostile‐submissive, and friendly‐submissive, and as less dominant and friendly‐dominant than patients with other principle disorders. After therapy, the 59 depressed patients whose significant others also provided data after treatment were perceived as less submissive (friendly‐submissive, submissive, hostile‐submissive) and more dominant and friendly‐dominant. Whereas a decrease in submissiveness and hostile‐submissiveness was associated with positive outcomes, decrease in friendly‐submissiveness was unrelated. Cluster analyses suggested four distinct interpersonal subgroups. We discuss these results in terms of interpersonal theory and interpersonal assessment in depression therapy.
Association of MR relaxation and cartilage deformation in knee osteoarthritis
Authors: Subburaj K. , Souza R. B. , Stehling C. , Wyman B. T. , Le Graverand‐Gastineau M.‐P. , Link T. M. , Li X. , Majumdar S. 
Published: December 2011

AbstractWe assessed the relationship between cartilage MR relaxation times and biomechanical response of tibiofemoral articular cartilage to physiological loading in healthy subjects and patients with osteoarthritis (OA). Female subjects above 40 years of age with (N1 = 20) and without (N2 = 10) OA were imaged on a 3T MR scanner using a custom made loading device. MR images were acquired with the knee flexed at 20° with and without a compressive load of 50% of the subject's bodyweight. The subjects were categorized based on the clinical MRI scoring of medial and lateral cartilage surfaces. Data were stratified twice into two equal groups (low and high) at the median value of T1ρ and T2 relaxation time. The change in contact area and cartilage deformation was measured within these groups. Paired Student's t‐test (α = 0.05) was used to analyze the effect of loading on contact area and deformation. The average area of the contact region in the medial compartment was significantly higher in OA subjects compared with normal subjects in both unloaded (314 ± 112 mm2 vs. 227 ± 106 mm2, p = 0.023) and loaded (425 ± 128 mm2 vs. 316 ± 107 mm2, p = 0.01) conditions. The overall relative change of cartilage thickness in the medial compartment was significantly higher than the lateral compartment (−5.3 ± 9.9% vs. −1.9 ± 9.2%, p = 0.042). When cartilage was divided into deep and superficial layers, superficial layers showed higher changes in relaxation time (T1ρ and T2) than the changes in relaxation time of whole cartilage (Normal: 12.5% vs. 6.9%; OA: 10.9% vs. 4.6%). The average T1ρ and T2 times, change in area of contact region, and change in cartilage thickness in subjects with OA were higher when compared to normal subjects. This study provides support for a relationship between the mechanical response of cartilage to physiological loading (cartilage‐on‐cartilage contact area and cartilage deformation) and MR relaxation times (T1ρ and T2) in both OA patients and normal subjects. © 2011 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:919–926, 2012
Intravenous electrocardiography helps inexperienced operators to place totally implantable venous access device more accurately
Authors: Wang Yi‐Chia , Huang Chi‐Hsiang , Lin Feng‐Sheng , Lin Wen‐Ying , Fan Shou‐Zen , Lin Chih‐Peng , Sun Wei‐Zen 
Published: December 2011

AbstractBackgroundProper tip position is a major determinant of totally implantable venous access device (TIVAD) outcome. The aim of this study is to analyze the potential utilization of intravenous electrocardiography (IV‐ECG) to help inexperienced operators for TIVAD placement.Patients and MethodsThis is a retrospective, observational, uni‐institutional study. 331 patients receiving TIVAD implantation from July 2008 to December 2008 were recruited. In IV‐ECG group, IV‐ECG was used to help decide catheter tip location and catheter length. In Landmark group, catheter length was decided by surface landmarks. Catheter tip position was confirmed by post‐operative supine chest X‐ray.ResultsThere were 153 patients in IV‐ECG group, and 178 patients in Landmark group. No immediate reoperation due to catheter mal‐position was noted in IV‐ECG group, but it happened in eight patients in Landmark group. In IV‐ECG group, 97.3% of the catheter tip located at proper position (within 2 cm from junction of right atrium and superior vena cava, as compared to 88.8% of the tip position in Landmark group was proper. The difference was statistically significant (P < 0.05). There was no complication associated with the use of IV‐ECG.ConclusionIV‐ECG is a safe and convenient method to help inexperienced operators placing TIVAD. J. Surg. Oncol. 2012; 105:848–851. © 2011 Wiley Periodicals, Inc.
Spatial ecology of white‐tailed deer fawns in the northern Great Plains: Implications of loss of conservation reserve program grasslands
Authors: Grovenburg Troy W. , Klaver Robert W. , Jenks Jonathan A. 
Published: December 2011

AbstractFew studies have evaluated how wildlife, and white‐tailed deer (Odocoileus virginianus) in particular, respond to Conservation Reserve Program (CRP) grasslands. We conducted a 3‐year study (2007–2009) to determine the influence of CRP on fawn ecology during a time of declining CRP enrollment. We captured and radiocollared 81 fawn white‐tailed deer during 15 May to 15 June 2007–2009 in north‐central South Dakota, collected 6,505 locations, and documented 70 summer home ranges. Mean summer home ranges increased temporally during 2007–2009 (P < 0.001) and corresponded to a 41% loss of CRP grasslands in the area (2.3% loss in land cover and approx. 21% loss in cover habitat in the study area) over the duration of the study. Additionally, mean movement between daily locations increased (P < 0.001) from 2007 to 2009. Analysis of covariance models indicated that change in CRP influenced home‐range size, and change in CRP and wheat influenced daily movement. Smaller home ranges and reduced movements were associated with greater quantity of CRP available to fawns, and increased movements were associated with more acreage of wheat available to fawns. Fawns shifted resource selection during the summer at a mean age ranging from 48.8 days to 58.6 days, and this shift was associated with height of corn (83–87 cm). During early summer, fawns consistently selected for CRP; selection of wheat progressed temporally from avoidance in 2007 to selection in 2009. During late summer, fawns consistently selected for corn habitat and used CRP at least in proportion to its availability. Reduction in CRP‐grasslands seemed to increase fawn home‐range size and daily movements and, influenced change in resource selection to wheat. Current legislation mandates continued decrease in CRP enrollment and concomitant increase in the planting of corn for ethanol production. Management of habitat throughout the grasslands of the Northern Great Plains that maximizes cover habitats would provide neonates with adequate cover for protection from predators. © 2011 The Wildlife Society.
Reproductive output and ultrasonography of an endangered population of East Pacific green turtles
Authors: Blanco Gabriela S. , Morreale Stephen J. , Vélez Elizabeth , Piedra Rotney , Montes Wilder M. , Paladino Frank V. , Spotila James R. 
Published: December 2011

AbstractReproductive output is one of the most relevant aspects of life history. We analyzed the reproductive output of the endangered East Pacific green turtle (Chelonia mydas) nesting in Nombre de Jesús, Costa Rica. We supplemented beach patrols with ultrasonography to estimate clutch frequency. With ultrasound scans, we classified the stage of turtle ovaries as: early stage (2 or more clutches), late stage (1 clutch), and depleted ovaries (no clutches). We calculated mean (±SD) estimated clutch frequency (ECF) to be 3.7 ± 1.8 (n = 24) and an adjusted frequency considering individual stage (ECFU; ECF + number of clutches remaining as observed in the last ultrasound) as 5.1 ± 1.3. This is greater than previously described for East Pacific green turtles. Greater individual output could be representative of a healthier population; but could also indicate a decrease in the estimate population numbers previously reported. © 2011 The Wildlife Society.
TouchPoints: The power of leading in the moment
Authors: Conant Doug , Norgaard Mette 
Published: December 2011

AbstractA leader's day is continuously punctuated by interruptions and interactions of all types. Rather than treating these events as nuisances, Conant and Norgaard advise to think of them as TouchPoints, opportunities to provide leadership and “to generate energy and direction around something that matters.” If you shift your perspective to thinking or saying, “How can I help?,” you have the potential to make a positive impact on your organization in a short moment of time. Your reputation, Conant and Norgaard contend, is shaped in these moments day after day.