bluuebunny/crossref_metadata_2025_split huggingface dataset:
ChemInform Abstract: From Ni‐YSZ to Sulfur‐Tolerant Anode Materials for SOFCs: Electrochemical Behavior, in situ Characterization, Modeling, and Future Perspectives
Authors: Cheng Zhe , Wang Jeng‐Han , Choi YongMan , Yang Lei , Lin M. C. , Liu Meilin 
Published: July 2012

AbstractReview: 217 refs.
Cooperative slit and netrin signaling in contralateralization of the mouse trigeminothalamic pathway
Authors: Mirza Rusella , Kivrak Beril G. , Erzurumlu Reha S. 
Published: July 2012

AbstractAscending somatosensory pathways are crossed pathways representing each side of the body in the contralateral neocortex. The principal sensory nucleus of the trigeminal nerve (PrV) relays the facial sensations to the contralateral somatosensory cortex via the ventrobasal thalamus. In the companion article (Kivrak and Erzurumlu [2012] J. Comp. Neurol. 12–0013) we described the normal development of the trigeminal lemniscal pathway in the mouse. In this study we investigated the role of midline axon navigation signals, the netrin and slit proteins. In situ hybridization assays revealed that both netrin and slit mRNAs are expressed along the midline facing the PrV axons and their receptors are expressed in developing PrV neurons. In wild‐type mouse embryos, PrV axons cross the midline and take a sharp rostral turn heading toward the contralateral thalamus. Examination of trigeminal lemniscal axons in dcc knockout mice revealed absence of midline crossing between E11 and E15. However, a few axons crossed the midline at E17 and reached the contralateral thalamus, resulting in a bilateral PrV lemniscal pathway at P0. We also found that slit1, ‐2 or ‐3 single or double knockout mice have impaired development of the trigeminal–lemniscal pathway. These include axon stalling along the midline, running within the midline, and recrossing of axons back to the site of origin. Collectively, our studies indicate a cooperative role for netrin and slit proteins in midline attraction and crossing behavior of the ascending facial somatosensory projections during development. J. Comp. Neurol. 521:312–325, 2013. © 2012 Wiley Periodicals, Inc.
Polytopic vector analysis of soil, dust, and serum samples to evaluate exposure sources of PCDD/Fs
Authors: Towey Timothy P. , Barabás Noémi , Demond Avery , Franzblau Alfred , Garabrant David H. , Gillespie Brenda W. , Lepkowski James , Adriaens Peter 
Published: July 2012

Abstract
As part of the University of Michigan Dioxin Exposure Study, soil, household dust, and serum samples were collected from more than 750 households in five populations around the city of Midland and in Jackson and Calhoun Counties, Michigan, USA. Polytopic vector analysis, a type of receptor model, was applied to better understand the potential sources of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans found in these samples and to quantify the contributions of the sources in each matrix across populations. The results indicated that source signatures found in soil are similar to those found in dust, reflecting various combustion profiles, pentachlorophenol, and graphite electrode sludge. The profiles associated with contamination in the Tittabawassee River, likely related to historical discharges from the Dow Chemical Company facility in Midland, exhibited the largest differences among the regional populations sampled. Differences in serum source contributions among the study populations were consistent with some of the regional differences observed in soil samples. However, the age trends of these differences suggested that they are related to past exposures, rather than ongoing sources. Environ. Toxicol. Chem. 2012; 31: 2191–2200. © 2012 SETAC
Identification of an intrahepatic transcriptional signature associated with self-limiting infection in the woodchuck model of hepatitis B
Authors: Fletcher Simon P. , Chin Daniel J. , Cheng Donavan T. , Ravindran Palanikumar , Bitter Hans , Gruenbaum Lore , Cote Paul J. , Ma Han , Klumpp Klaus , Menne Stephan 
Published: July 2012

Abstract
The woodchuck model of hepatitis B virus (HBV) infection displays many characteristics of human infection and has particular value for characterizing the host immune responses during the development of chronic infection. Using the newly developed custom woodchuck microarray platform, we compared the intrahepatic transcriptional profiles of neonatal woodchucks with self-limiting woodchuck hepatitis virus (WHV) infection to those woodchucks progressing to persistent WHV infection. This revealed that WHV does not induce significant intrahepatic gene expression changes during the early-acute stage of infection (8 weeks), suggesting it is a stealth virus. At the mid-acute phase of infection (14 weeks), resolution was associated with induction of a prominent cytotoxic T-cell signature. Strikingly, this was accompanied by high-level expression of PD-1 and various other inhibitory T-cell receptors, which likely act to minimize liver damage by cytotoxic T cells during viral clearance. In contrast to the expression of perforin and other cytotoxic effector genes, the interferon-γ (IFN-γ) signaling response in the mid-acute phase was comparable to that in chronically infected adult animals. The absence of a strong IFN-α/β transcriptional response indicated that type I IFN is not a critical mediator of self-limiting infection. Nevertheless, a number of antiviral genes, including viperin, were differentially expressed during resolving infection, suggesting that a subset of IFN-stimulated genes (ISG) may play a role in the control of WHV replication. Conclusion: We identified new immune pathways associated with the clearance of hepadnavirus infection revealing novel molecular targets with potential for the therapeutic treatment of chronic hepatitis B. (Hepatology 2013)
Simultaneous knockdown of multiple ligands of innate receptor NKG2D prevents natural killer cell–mediated fulminant hepatitis in mice
Authors: Huang Mei , Sun Rui , Wei Haiming , Tian Zhigang 
Published: July 2012

Abstract
NKG2D activation plays an important role in initiating and maintaining liver inflammation, and blockade of NKG2D recognition becomes a promising approach to alleviate liver inflammation. Treatment by silencing NKG2D ligands on hepatocytes, but not NKG2D on circulating immune cells, is more liver-specific, and simultaneous knockdown of multiple NKG2D ligands on hepatocytes will be more efficient in liver disease intervention. Here, we constructed a single vector that could simultaneously express multiple short hairpin RNAs (shRNAs) against all murine NKG2D ligands including Rae1, Mult1, and H60. After hydrodynamic injection of plasmid containing the three shRNA sequences (shRae1-shMult1-shH60), also called pRNAT-shRMH, we found the expression of all three NKG2D ligands on hepatocytes was downregulated both on messenger RNA and protein levels. Moreover, natural killer (NK) cell–mediated NKG2D-dependent fulminant hepatitis of the mice was alleviated, along with inactivation of hepatic NK cells, by pRNAT-shRMH if compared with its counterpart RNA interference vectors against single or double ligands. The therapeutic efficacy of pRNAT-shRMH was equivalent to that of injecting three monoclonal antibodies against Rae1, Mult1, and H60. For better in vivo application, we constructed a recombinant adenovirus containing pRNAT-shRMH (called Ad-RMH) with efficient hepatotropic infection capacity and observed that Ad-RMH intravenous injection exerted a similar therapeutic efficiency as plasmid pRNAT-shRMH hydrodynamic injection. Noticeably, simultaneous knockdown of multiple human NKG2D ligands (MICA/B, ULBP2, and ULBP3) also significantly attenuated NK cell cytolysis against human NKG2D ligand-positive hepatocyte L-02 cells, suggesting a possible translation into human settings. Conclusion: Simultaneous knockdown of multiple ligands of NKG2D prevents NK cell–mediated fulminant hepatitis and is a potential therapeutic approach to treat liver diseases. (Hepatology 2013)
Retraction of “Using ergonomic digital human modeling in evaluation of workplace design and prevention of workrelated musculoskeletal disorders onboard small fishing vessel”
Authors: Álvarez‐Casado Enrique , Zhang Bing , Sandoval Sonia Tello , Pedro Mondelo 
Published: July 2012

AbstractThe following article from Human Factors and Ergonomics in Manufacturing & Service Industries, “Using ergonomic digital human modeling in evaluation of workplace design and prevention of workrelated musculoskeletal disorders onboard small fishing vessel,” by Enrique Álvarez‐Casado, Bing Zhang, Sonia Tello Sandoval and Mondelo Pedro, published online on October 11, 2011 in Wiley Online Library (www.onlinelibrary.wiley.com), has been retracted by agreement between the authors, the journal Editors‐in‐Chief, Waldemar Karwowski and Gavriel Salvendy, and Publisher Wiley Periodicals, Inc. The retraction has been agreed as these articles were mistakenly published after being rejected.
Retraction of “The effect of LED lighting on color discrimination and preference of the elderly”
Authors: Cheng Wenting , Ju Jiaqi , Sun Yaojie , Lin Yandan 
Published: July 2012

AbstractThe following article from Human Factors and Ergonomics in Manufacturing & Service Industries, “The Effect of LED Lighting on Color Discrimination and Preference of the Elderly,” by Wenting Cheng, Jiaqi Ju, Yaojie Sun and Yandan Lin, published online on December 12, 2011 in Wiley Online Library (www.onlinelibrary.wiley.com), has been retracted by agreement between the authors, the journal Editors‐in‐Chief, Waldemar Karwowski and Gavriel Salvendy, and Publisher Wiley Periodicals, Inc. The retraction has been agreed as these articles were mistakenly published after being rejected.
Retraction of “The influence of elderly vision degradation on subjective rating of floor slipperiness”
Authors: Hsu Yao‐Wen 
Published: July 2012

AbstractThe following article from Human Factors and Ergonomics in Manufacturing & Service Industries, “The Influence of Elderly Vision Degradation on Subjective Rating of Floor Slipperiness,” by Yao‐Wen Hsu, published online on December 12, 2011 in Wiley Online Library (www.onlinelibrary.wiley.com), has been retracted by agreement between the authors, the journal Editors‐in‐Chief, Waldemar Karwowski and Gavriel Salvendy, and Publisher Wiley Periodicals, Inc. The retraction has been agreed as these articles were mistakenly published after being rejected.
Retraction of “Developing an integrated display of health data for aging in place”
Authors: Gong Yang , Chandra Arpita 
Published: July 2012

AbstractThe following article from Human Factors and Ergonomics in Manufacturing & Service Industries, “Developing an Integrated Display of Health Data for Aging in Place,” by Yang Gong and Arpita Chandra, published online on October 20, 2011 in Wiley Online Library (www.onlinelibrary.wiley.com), has been retracted by agreement between the authors, the journal Editors‐in‐Chief, Waldemar Karwowski and Gavriel Salvendy, and Publisher Wiley Periodicals, Inc. The retraction has been agreed as these articles were mistakenly published after being rejected.
Fabrication of TiO2 nanotubes on porous titanium scaffold and biocompatibility evaluation in vitro and in vivo
Authors: Fan Xingping , Feng Bo , Liu Zhiyuan , Tan Jing , Zhi Wei , Lu Xiong , Wang Jianxin , Weng Jie 
Published: July 2012

AbstractPorous titanium was modified by anodic oxidation and heat treatment method. Scanning electron microscopy and X‐ray diffraction examinations revealed that the modified surface of porous titanium was covered by anatase nanotubes. In vitro, the bioactivity of specimens before and after modification was evaluated by immersing into the double‐concentration simulated body fluid for 7 days. The porous titanium specimens were implanted into the femurs of dogs for 3 months. The osteointegration of the implants was investigated by push‐out test and histological examination. The results showed that the porous titanium with anatase nanotubes has the superior ability of apatite formation and a higher push‐out force when compared with the other implants. The histological analysis indicated that the implant with anatase nanotubes had excellent ability to facilitate the osteointegration in vivo. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:3422–3427, 2012.
Characterization and degradation of elastomeric four‐armed star copolymers based on caprolactone and L‐lactide
Authors: Kong Jen Fong , Lipik Vitali , Abadie Marc J. M. , Roshan Deen G. , Venkatraman Subbu S. 
Published: July 2012

AbstractAlthough biodegradable polymers have found extensive applications in medical areas, there are limited reports that show elastomeric behavior. In this work, a biodegradable, elastomeric polymer is demonstrated from a four‐armed star copolymer. With a fixed middle core composition, comprising caprolactone (CL) and L‐lactide (LA), an elastomer is obtained by increasing the polylactide (PLA) end block lengths to obtain sufficient end block crystallinity. This increase suppressed the middle core's crystallinity yet ensured cocrystallization of the PLA ends of individual star copolymer chains to form a three‐dimensional network via physical crosslinking. Cyclic and creep test of the star copolymers showed that at least 75% of recovery was achieved. Degradation study of the copolymer showed that degradation first occurred in the caprolactone‐co‐lactide (CLLA) core, followed by degradation in the PLA ends. Chain scission in the middle core resulted in immediate formation of CL crystals within the core and increased crystallinity over time, in both CLLA core and PLA ends. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:3436–3445, 2012.
Effects of surface functionalization of PLGA membranes for guided bone regeneration on proliferation and behavior of osteoblasts
Authors: Chen Gang , Xia Yang , Lu Xiaoli , Zhou Xuefeng , Zhang Feimin , Gu Ning 
Published: July 2012

AbstractCovalent immobilization of bioactive compounds onto modified poly lactic‐co‐glycolic acid (PLGA) surfaces is being rapidly developed in tissue engineering, but the compounds and the grafting procedure require optimization. Here, PLGA membranes were grafted with various ratios of collagen/chitosan (COL/CHI) composites after modification by polydopamine and then analyzed using attenuated total reflectance Fourier transform infrared spectroscopy, field emission scanning electron microscopy, and a contact angle meter. Mechanical properties of the membranes were examined by tensile testing. Proliferation of osteoblastic cell line MC3T3‐E1 cultured on the membranes was examined by MTT (3‐(4, 5‐dimethylthiozole‐2‐yl)‐2, 5‐diphenyltetrazolium bromide) and flow cytometric analysis. Effects on cell behavior, including cytotaxis, adhesion, and migration, were further investigated by continuous time‐lapse imaging for 8 h. The COL/CHI composites were successfully immobilized onto PLGA surfaces. PLGA mainly contributed the mechanical properties, while COL/CHI played a major role in bioactivity. COL facilitated cell adhesion and spread, but the addition of CHI decreased both. A critical ratio of COL/CHI (2:1) above which the addition of CHI only slightly impacted cell proliferation was found. The results should be useful for combining versatile materials from different origins to construct guided bone regeneration membranes and to further optimize the ratio of COL/CHI composites. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 101A:44–53, 2013.
Microarchitecture and bone quality in the human calcaneus: Local variations of fabric anisotropy
Authors: Souzanchi Mohammad F , Palacio-Mancheno Paolo , Borisov Yury A , Cardoso Luis , Cowin Stephen C 
Published: July 2012

Abstract
The local variability of microarchitecture of human trabecular calcaneus bone is investigated using high-resolution micro–computed tomography (µCT) scanning. The fabric tensor is employed as the measure of the microarchitecture of the pore structure of a porous medium. It is hypothesized that a fabric tensor–dependent poroelastic ultrasound approach will more effectively predict the data variance than will porosity alone. The specific aims of the present study are as follows: (1) to quantify the morphology and local anisotropy of the calcaneus microarchitecture with respect to anatomical directions; (2) to determine the interdependence, or lack thereof, of microarchitecture parameters, fabric, and volumetric bone mineral density (vBMD); and (3) to determine the relative ability of vBMD and fabric measurements in evaluating the variance in ultrasound wave velocity measurements along orthogonal directions in the human calcaneus. Our results show that the microarchitecture in the analyzed regions of human calcanei is anisotropic, with a preferred alignment along the posterior-anterior direction. Strong correlation was found between most scalar architectural parameters and vBMD. However, no statistical correlation was found between vBMD and the fabric components, the measures of the pore microstructure orientation. Therefore, among the parameters usually considered for cancellous bone (ie, classic histomorphometric parameters such as porosity, trabecular thickness, number and separation), only fabric components explain the data variance that cannot be explained by vBMD, a global mass measurement, which lacks the sensitivity and selectivity to distinguish osteoporotic from healthy subjects because it is insensitive to directional changes in bone architecture. This study demonstrates that a multidirectional, fabric-dependent poroelastic ultrasound approach has the capability of characterizing anisotropic bone properties (bone quality) beyond bone mass, and could help to better understand anisotropic changes in bone architecture using ultrasound. © 2012 American Society for Bone and Mineral Research.
Dopaminergic differentiation using pluripotent stem cells
Authors: Momčilović Olga , Montoya‐Sack Justine , Zeng Xianmin 
Published: July 2012

AbstractParkinson's disease (PD) is the second most common neurodegenerative disorder. The motor symptoms of PD are caused by the loss of dopaminergic (DA) neurons in the substantia nigra pars compacta of mesencephalon. The causes for death of DA neurons are not well understood, but the strongest risk factor is increasing age. There is no cure currently available for PD, and treatment is limited to management of PD symptoms in patients. Primary DA neurons are virtually unobtainable from living patients and animal studies have proven inadequate for studying the mechanism of PD development. Pluripotent stem cells (PSC) are primary self‐renewing cells capable of differentiating into all cell types of an organism, including DA neurons. PSCs represent an abundant source of cells that can be genetically modified or isolated from patients with complex diseases, enabling the production of large quantities of DA neurons for disease modeling, drug screening, and gene function studies. Furthermore, since PD arises as a result of deterioration of DA neurons in a specific brain region, it has been suggested that a relatively small number of cells could restore normal function. PSCs could provide a source of DA neurons for cell replacement therapy. In this Prospects article, we focus on the development and in vitro derivation of DA neurons from PSCs, as well as current applications of the technological advances, with the emphasis on future directions and efforts in the field. J. Cell. Biochem. 113: 3610–3619, 2012. © 2012 Wiley Periodicals, Inc.
NELL‐1 promotes cell adhesion and differentiation via integrinβ1
Authors: Shen Jia , James Aaron W. , Chung Jonguk , Lee Kiho , Zhang James B. , Ho Stephanie , Lee Kevin S. , Kim Toyong M. , Niimi Tomoaki , Kuroda Shun'ichi , Ting Kang , Soo Chia 
Published: July 2012

AbstractNELL‐1 (Nel‐like molecule‐1) is a secreted osteogenic growth factor first identified in human craniosynostosis (CS) patients. NELL‐1 protein has been observed to promote bone and cartilage differentiation and to suppress adipogenesis in both in vitro and in vivo models. Despite these findings, the cell surface receptors of NELL‐1 have remained unknown. In this study, we observed for the first time that NELL‐1 promotes cell adherence in multiple cell lines, including ST2, C3H10T1/2, M2‐10B4, ATDC5, and MC3T3 cells. Additionally, we found that NELL‐1 binds to extracellular Integrinβ1 and induces cell focal adhesion. By utilizing siRNA methods, we determined that NELL‐1 cell surface binding and enhanced cell attachment were dependent on Integrinβ1 expression. Finally, we observed that pre‐coating of culture dishes or PLGA (polylactic‐co‐glycolic acid) scaffold with NELL‐1 resulted in a significant increase in both cell attachment and osteogenic differentiation. Our results identify for the first time a cell surface target of NELL‐1, Integrinβ1, and elucidate new functions of NELL‐1 in promoting cell adherence and osteogenic differentiation. J. Cell. Biochem. 113: 3620–3628, 2012. © 2012 Wiley Periodicals, Inc.
Knockdown of nucleosome assembly protein 1‐like 1 promotes dimethyl sulfoxide‐induced differentiation of P19CL6 cells into cardiomyocytes
Authors: Li Lu , Gong Hui , Yu Hongxiu , Liu Xiaohui , Liu Qingping , Yan Guoquan , Zhang Yang , Lu Haojie , Zou Yunzeng , Yang Pengyuan 
Published: July 2012

AbstractTransplantation of cardiomyocytes derived from stem cells is a promising option for cardiac repair. However, how to obtain efficient cardiomyocytes from stem cells is still a great challenge. Understanding of the mechanism that regulates the cardiac differentiation of stem cells is necessary for the effective induction of cardiomyocytes. A clonal derivative named P19CL6 cells can easily differentiate into cardiomyocytes with 1% dimethyl sulfoxide (DMSO) treatment, which offers a valuable model to study cardiomyocytes differentiation in vitro. In this study, the isobaric tags for relative and absolute quantitation (iTRAQ) proteomics were performed to identify proteins associated with cardiomyocytes differentiation of P19CL6 cells induced by DMSO. Out of 543 non‐redundant proteins identified, 207 proteins showed significant changes during differentiation with ≥1.2‐fold or ≤0.83‐fold changes cut‐offs. Nine proteins were confirmed by the quantitative real‐time polymerase chain reaction (qRT‐PCR) and Western blot analysis respectively. Notably, broad consistency was well showed between mRNA and protein expression for down‐regulation of nucleosome assembly protein 1‐like 1 (Nap1l1). Further study revealed that knockdown of Nap1l1 by stable transfection of shRNA vector significantly accelerated DMSO‐induced cardiomyocytes differentiation of P19CL6 cells characterized by increases in expression of cardiac specific transcription factors, genes, and proteins (GATA4, MEF‐2C, ANP, BNP, cTNT, and β‐MHC). Therefore, Nap1l1 is a novel protein that regulates cardiomyocytes differentiation of P19CL6 cells induced by DMSO. J. Cell. Biochem. 113: 3788–3796, 2012. © 2012 Wiley Periodicals, Inc.