bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modulation of Cytokine and Chemokine Secretions in Rhesus Monkey Trophoblast Co‐Culture With Decidual but not Peripheral Blood Monocyte–Derived Macrophages
Authors: Rozner Ann E. , Dambaeva Svetlana V. , Drenzek Jessica G. , Durning Maureen , Golos Thaddeus G. 
Published: January 2011

Citation  Rozner AE, Dambaeva SV, Drenzek JG, Durning M, Golos TG. Modulation of cytokine and chemokine secretions in rhesus monkey trophoblast co‐culture with decidual but not peripheral blood monocyte–derived macrophages. Am J Reprod Immunol 2011; 66: 115–127Problem  Decidual macrophages are thought to promote pregnancy success, in part through interactions with invading trophoblast cells in hemochorial placentation. However, the factors that constitute this regulatory cross talk are not well understood.Method of study  Rhesus monkey decidual and peripheral blood–derived macrophages were co‐cultured with primary Rhesus trophoblasts. Macrophage functions including cell‐surface marker expression, antigen uptake and processing, in vitro migration, and cytokine and chemokine secretions were evaluated.Results  While most macrophage functions were unchanged by trophoblast co‐culture, changes in the secretion of selected cytokines and the migration of trophoblasts were noted when decidual (but generally, not peripheral blood monocyte–derived) macrophages were cultured with trophoblasts. In addition, basal secretion differed significantly between peripheral blood–derived and decidual macrophages for a broad spectrum of cytokines. When trophoblasts were pre‐treated with an anti‐Mamu‐AG antibody, 25D3, there was no change in cytokine or chemokine secretion.Conclusion  Macrophage cytokine expression can be modulated by trophoblast co‐culture, but it remains unclear how Mamu‐AG is involved.
Regulation of Costimulatory Signal in Maternal–Fetal Immune Tolerance
Authors: Jin Li‐Ping , Fan Deng‐Xuan , Li Da‐Jin 
Published: January 2011

Citation  Jin L‐P, Fan D‐X, Li D‐J. Regulation of costimulatory signal in maternal‐fetal immune tolerance. Am J Reprod Immunol 2011; 66: 76–83A pregnancy is associated with modifications in the immune status of the mother, but the mechanisms are not well understood. Several observations have indicated that CD28/CTLA‐4 and B7‐1/B7‐2 are involved in the maternal–fetal immune regulation. This review aims to recapitulate our current knowledge concerning the role of CD28/CTLA‐4 and B7‐1/B7‐2 in maternal–fetal immune regulation. Several studies suggest that up‐regulation of B7‐2 and/or CD28 and/or down‐regulation of CTLA‐4 are correlated with the occurrence of pregnancy loss. Therefore, an accurate expression of costimulatory molecules at the maternal–fetal interface may ensure that the decidual cells do not elicit a ‘danger’ signal to the maternal immune system, perhaps instead contributing to the establishment of immune tolerance in vivo. It is showed that costimulation blockade with anti‐B7 mAbs results in altered allogeneic T‐cell response and overcomes increased maternal rejection to the fetus, which improves fetus growth in the abortion‐prone system. These findings suggest that the anti‐B7‐treated T cells not only function as potent suppresser cells but also exert immunoregulatory effect on the maternal T cells. This procedure might be potentially useful to immunotherapy for human recurrent spontaneous abortion.
Expression Profiles of Peripheral CD160+ Lymphocytes During the Course of Healthy Human Pregnancy
Authors: Barakonyi Aliz , Weisdorn Renata , Miko Eva , Varga Peter , Bodis Jozsef , Szekeres‐Bartho Julia , Szereday Laszlo 
Published: January 2011

Citation
Barakonyi A, Weisdorn R, Miko E, Varga P, Bodis J, Szekeres‐Bartho J, Szereday L. Expression profiles of peripheral CD160+ lymphocytes during the course of healthy human pregnancy. Am J Reprod Immunol 2011; 66: 137–142Problem  CD160 receptor is expressed by natural killer (NK) and T‐cell subsets, and after activation, it could enhance cytotoxicity or pro‐inflammatory cytokine production on NK cells. Here, we investigated the phenotype of peripheral CD160+ cells during healthy pregnancy.Method of study  We analyzed the expression of CD69 activation marker, gamma/delta TCR, and NKG2A or NKG2D NK cell receptors on CD160+ lymphocytes of non‐pregnant and healthy pregnant women at four different stages of pregnancy by flow cytometry.Results  In our hands, CD160 receptor‐positive lymphocytes were present during pregnancy; however, they had different characteristics depending on gestational age. During implantation, CD160+ cells showed low activation rate, decreased NK receptor expression while 40% of Vδ2 + T cells expressed CD160 receptor. In turn, all the above parameters increased as pregnancy proceeds.Conclusion  Our results indicate that CD160+ lymphocytes could be able to play a role in the maintenance of healthy pregnancy.
Clinical appearance, differential diagnoses and therapeutical options of chondrodermatitis nodularis chronica helicis Winkler
Authors: Wagner Gunnar , Liefeith Jan , Sachse Michael Max 
Published: January 2011

SummaryThe article on chondrodermatitis nodularis chronica helicis summarizes various clinical pictures and differential diagnoses of this entity. This lesion is usually characterized by a solid or cystic nodule but ulcerations or crusts may also occur. The most common differential diagnoses include benign or malignant tumours, which are often confused with chondrodermatitis nodularis chronica helicis. The characteristic pain associated with this condition may serve as an important diagnostic clue in order to rule out other differential diagnoses. The therapy of chondrodermatitis nodularis chronica helices encompasses several conservative as well as surgical treatment options.
Modulation of F0F1‐ATP synthase activity by cyclophilin D regulates matrix adenine nucleotide levels
Authors: Chinopoulos Christos , Konràd Csaba , Kiss Gergely , Metelkin Eugeniy , Töröcsik Beata , Zhang Steven F. , Starkov Anatoly A. 
Published: January 2011

Cyclophilin D was recently shown to bind to and decrease the activity of F0F1‐ATP synthase in submitochondrial particles and permeabilized mitochondria [Giorgio V et al. (2009) J Biol Chem, 284, 33982–33988]. Cyclophilin D binding decreased both ATP synthesis and hydrolysis rates. In the present study, we reaffirm these findings by demonstrating that, in intact mouse liver mitochondria energized by ATP, the absence of cyclophilin D or the presence of cyclosporin A led to a decrease in the extent of uncoupler‐induced depolarization. Accordingly, in substrate‐energized mitochondria, an increase in F0F1‐ATP synthase activity mediated by a relief of inhibition by cyclophilin D was evident in the form of slightly increased respiration rates during arsenolysis. However, the modulation of F0F1‐ATP synthase by cyclophilin D did not increase the adenine nucleotide translocase (ANT)‐mediated ATP efflux rate in energized mitochondria or the ATP influx rate in de‐energized mitochondria. The lack of an effect of cyclophilin D on the ANT‐mediated adenine nucleotide exchange rate was attributed to the ∼ 2.2‐fold lower flux control coefficient of the F0F1‐ATP synthase than that of ANT, as deduced from measurements of adenine nucleotide flux rates in intact mitochondria. These findings were further supported by a recent kinetic model of the mitochondrial phosphorylation system, suggesting that an ∼ 30% change in F0F1‐ATP synthase activity in fully energized or fully de‐energized mitochondria affects the ADP–ATP exchange rate mediated by the ANT in the range 1.38–1.7%. We conclude that, in mitochondria exhibiting intact inner membranes, the absence of cyclophilin D or the inhibition of its binding to F0F1‐ATP synthase by cyclosporin A will affect only matrix adenine nucleotides levels.Structured digital abstract
F0F1‐ATPase beta and CypD physically interact by cross‐linking study (View interaction)
Comparative study of cisplatin and epirubicin in transcatheter arterial chemoembolization for hepatocellular carcinoma
Authors: Yamanaka Kenya , Hatano Etsuro , Narita Masato , Taura Kojiro , Yasuchika Kentaro , Nitta Takashi , Arizono Shigeki , Isoda Hiroyoshi , Shibata Toshiya , Ikai Iwao , Sato Tosiya , Uemoto Shinji 
Published: January 2011

Aim:  Transcatheter arterial chemoembolization (TACE) is an established treatment for unresectable hepatocellular carcinoma (HCC). However, it is unclear which chemotherapeutic agent should be selected for TACE. The aim of this study was to compare the efficacy of cisplatin (CDDP) with that of epirubicin (EPI) in TACE for patients with unresectable or relapsed HCC.Methods:  We performed a historical cohort study involving 131 patients treated with a first TACE, defined as either an initial treatment for previously untreated HCC or a first treatment for relapsed HCC after curative resections or ablations. Efficacy was estimated as the response rate (RR) and it was adjusted for the confounding factors that were defined in this study.Results:  The RR were 62.5% (20/32) for the first TACE with CDDP and 51.5% (51/99) for that with EPI. In the adjusted analysis for a history of hepatectomy, percutaneous treatment combined with TACE and tumor factors, the odds ratio was 1.72 (95% confidence interval [CI] = 0.70–4.48). However, a test for interaction between the number of tumors and the chemotherapeutic agent was statistically significant (P = 0.016). In multiple HCC, the RR were 66.7% (10/17) for CDDP and 39.6% (30/46) for EPI. The odds ratio was 4.11 (95% CI = 1.14–17.2).Conclusion:  CDDP may be more effective than EPI in TACE for multiple HCC. A randomized controlled study is needed to clarify the efficacy of CDDP in TACE in patients with multiple HCC.
Studies on virus kinetics using infectious fluorescence‐tagged hepatitis C virus cell culture
Authors: Yamamoto Machi , Sakamoto Naoya , Nakamura Tetsuya , Itsui Yasuhiro , Nakagawa Mina , Nishimura‐Sakurai Yuki , Kakinuma Sei , Azuma Seishin , Tsuchiya Kiichiro , Kato Takanobu , Wakita Takaji , Watanabe Mamoru 
Published: January 2011

Aim:  Studies of the complete hepatitis C virus (HCV) life cycle have become possible with the development of a HCV‐JFH1 cell culture system.Methods:  In this study, we constructed two fluorescence protein‐tagged recombinant JFH1 virus clones, JFH1‐EYFP and JFH1‐AsRed, as well as two corresponding clones with adaptive mutations, JFH1‐EYFP mutant and JFH1‐AsRed mutant, that and were as effective as JFH1 in producing infectious virus particles, and investigated their viral infection life cycles.Results:  After infection of the fluorescence‐tagged mutant viruses, infected cells increased exponentially. In cells, EYFP or AsRed and NS5A were expressed as a fusion protein and co‐localized in core proteins. The rate of the cell–cell spread was dependent on the cell densities with a maximum of 102.5/day. Treatment of cells with interferon or a protease inhibitor suppressed expansion of virus‐positive cells.Conclusion:  Taken together, these results indicate that fluorescence‐tagged HCV is a useful tool to study virus infection life cycles and to assist in the search for novel antiviral compounds.
No associations of polymorphisms in ADPRT with hepatitis B virus clearance and hepatocellular carcinoma occurrence in a Korean population
Authors: Lee Jin Sol , Kim Jeong‐Hyun , Park Byung‐Lae , Cheong Hyun Sub , Koh InSong , Kim Jason Yong‐ha , Park Tae Joon , Pasaje Charisse Flerida , Bae Joon Seol , Lee Hyo‐Suk , Kim Yoon Jun , Shin Hyoung Doo 
Published: January 2011

Aim:  The human adenosine diphosphate ribosyl transferase (ADPRT) gene might significantly affect cancer by encoding poly(ADP‐ribose) polymerase 1 enzyme (PARP‐1) and promoting an important role in cellular responses to DNA damage, genomic stabilization and regulation of tumor suppressor genes. We explored whether polymorphisms of ADPRT affect clearance of hepatitis B virus (HBV) infection or risk of hepatocellular carcinoma (HCC) occurrence in a Korean HBV cohort.Methods:  Genotyping was performed in a total of 1066 subjects composed of 434 spontaneously recovered (SR) subjects as normal controls and 632 chronic carriers (CC) of HBV who were further classified into 325 patients with liver cirrhosis (LC)/chronic hepatitis (CH) and 307 patients with HCC.Results:  Logistic analyses of six common single nucleotide polymorphisms (SNP) and their haplotypes revealed that none of the polymorphisms were significantly associated with clearance of HBV infection and HCC occurrence, except for nominal evidence of association between haplotype 2 (ht2) with HBV clearance (P = 0.05). In the analysis of age of HCC occurrence which is an important factor in disease progression to HCC, results from Cox proportional hazards showed that none of the variants were significantly associated with onset age of HCC occurrence, although a nominal signal in ht4 (P = 0.03, but Pcorr > 0.05) was initially detected.Conclusion:  Although ADPRT is an important gene for cellular responses and tumor regulations, our study provides evidence that ADPRT variations do not affect HBV clearance and HCC occurrence.
Neurological Causes of Diaphragmatic Paralysis in 11 Alpacas (Vicugna pacos)
Authors: Byers S. , Barrington G. , Nelson D. , Haldorson G. , Holt T. , Callan R. 
Published: January 2011

Background:Diaphragmatic paralysis is a relatively uncommon medical condition in animals not reported in alpacas.Objectives:Describe the signalment, physical examination, diagnostic testing, clinical, and histopathologic findings related to diaphragmatic paralysis in alpacas.Animals:Eleven alpacas with spontaneous diaphragmatic paralysis.Methods:A retrospective study examined medical records from a 10‐year period and identified 11 alpacas with confirmed diaphragmatic paralysis admitted to Washington State University and Colorado State University Veterinary Teaching Hospitals between September 2003 and October 2009.Results:The 11 alpacas ranged in age from 2 to 12 months. Fluoroscopic imaging confirmed the presence of bilateral diaphragmatic paralysis in the 7 alpacas that were imaged. Arterial blood gas analyses showed hypercapnea, hypoxemia, and low oxygen saturation. Seven alpacas died or were euthanized between 2 and 60 days after onset of respiratory signs. Histopathologic examination of tissues found phrenic nerve degeneration in the 6 alpacas that were necropsied and additional long nerves examined demonstrated degeneration in 2 of these animals. Two animals had spinal cord lesions and 2 had diaphragm muscle abnormalities. No etiologic agent was identified in the alpacas.Conclusions and Clinical Importance:The etiology for diaphragmatic paralysis in these alpacas is unknown. A variety of medical treatments did not appear to alter the outcome.
Bronchoalveolar Lavage Fluid Cytology and Cytokine Messenger Ribonucleic Acid Expression of Racehorses with Exercise Intolerance and Lower Airway Inflammation
Authors: Lavoie J.P. , Cesarini C. , Lavoie‐Lamoureux A. , Moran K. , Lutz S. , Picandet V. , Jean D. , Marcoux M. 
Published: January 2011

Background: There is limited information relating bronchoalveolar lavage (BAL) cytology and cytokine messenger ribonucleic acid (mRNA) expression in racehorses with inflammatory airway disease (IAD).Hypothesis and Objective: We hypothesize that cytokine expression in BAL cells would correlate with cytology. Thus, we evaluated the mRNA expression of selected cytokines in BAL cells in racehorses with exercise intolerance and lower airway inflammation.Animals: Thirty‐one client‐owned Standardbred racehorses with exercise intolerance.Methods: Prospective, observational study. Cells were obtained by BAL, and mRNA expression of interleukin (IL)‐1β, IL‐4, IL‐8, tumor necrosis factor (TNF)‐α, and interferon (IFN)‐γ was determined by reverse transcription quantitative polymerase chain reaction (RT‐qPCR).Results: Nine horses had normal BAL cell differential cytology (Controls), while 22 horses had evidence of IAD based on BAL fluid cytology. Relative expressions of TNF‐α/glyceraldehyde 3‐phosphate dehydrogenase (GAPDH; 0.0092 ± 0.010 versus 0.0045 ± 0.005, P= .034), IL‐4/GAPDH (0.001 ± 0.002 versus 0.0003 ± 0.0003, P= .029), and IFN‐γ/GAPDH (0.0027 ± 0.003 versus 0.0009 ± 0.001, P= .028) were greater in horses with IAD compared with controls. Furthermore, IL‐4/GAPDH (0.001 ± 0.002 versus 0.0002 ± 0.0003, P < .0001) and IFN‐γ/GAPDH (0.003 ± 0.003 versus 0.001 ± 0.001, P= .002) mRNA expression was increased in horses with increased metachromatic cell counts compared with horses with normal metachromatic cell counts. Only the mRNA expression of IL‐1β/GAPDH (1.1 ± 0.7 versus 0.3 ± 0.3, P= .045) was increased with airway neutrophilia.Conclusions and Clinical Importance: Differences in gene expression were associated with the presence of IAD and with specific cell types present in airway secretions of Standardbred racehorses with poor performance. These findings suggest that different pathophysiological pathways are implicated in IAD.
Comparison of Water Manometry to 2 Commercial Electronic Pressure Monitors for Central Venous Pressure Measurement in Horses
Authors: Norton J.L. , Nolen‐Walston R.D. , Underwood C. , Slack J. , Boston R. , Dallap B.L. 
Published: January 2011

Background: Central venous pressure (CVP) customarily has been measured in veterinary patients with water manometry. However, many institutions are now using stallside electronic monitors in both anesthesia and intensive care units for many aspects of patient monitoring.Hypothesis: Electronic stall side monitoring devices will agree with water manometry for measurement of CVP in horses.Animals: Ten healthy adult horses from the university research herd.Methods: Central venous catheters were placed routinely, and measurements were obtained in triplicate with each of the 3 methods every 12 hours for 3 days. Data were analyzed by a Lin concordance correlation coefficient and modified Bland‐Altman limits of agreement, with all devices compared pairwise.Results: Compared with water manometry, agreement (bias) of the Passport was −1.94 cmH2O (95% limits of agreement, −8.54 to 4.66 cmH2O) and of the Medtronic was −1.83 cmH2O (95% limits of agreement, −8.60 to 4.94 cmH2O). When compared with the Passport, agreement of the data obtained with the Medtronic was 0.27 cmH2O (95% limits of agreement, −4.39 to 4.93 cmH2O).Conclusions and Clinical Importance: These data show that both electronic monitors systematically provide measurements that are approximately 2 cmH2O lower than water manometry, but differences between the 2 electronic devices are small enough (< 0.5 cmH2O) to be considered clinically unimportant. This discrepancy should be taken into account when interpreting data obtained with these monitoring devices.
Effect of Parvoviral Enteritis on Plasma Citrulline Concentration in Dogs
Authors: Dossin O. , Rupassara S.I. , Weng H.‐Y. , Williams D.A. , Garlick P.J. , Schoeman J.P. 
Published: January 2011

Background: Plasma citrulline concentration is a reliable marker of global enterocyte mass in humans and is markedly decreased in diffuse small intestinal diseases. However, the relationship between acute intestinal damage and plasma citrulline concentration in dogs has never been documented.Hypothesis: That dogs with parvoviral enteritis have a lower plasma citrulline concentration than healthy dogs and that plasma citrulline concentration is a predictor of death in puppies with parvoviral enteritis.Animals: Sixty‐one dogs with spontaneous parvoviral enteritis and 14 healthy age‐matched control dogs.Methods: Observational cohort study. Plasma citrulline concentration was measured by liquid chromatography and tandem mass spectrometry in blood samples collected at admission and each day until death or discharge from the hospital. Parvovirus enteritis was confirmed by electron microscopy on a fecal sample.Results: Median (interquartile range) plasma citrulline concentrations at admission were 2.8 μmol/L (range: 0.3, 49.0; P < .001 versus controls) in survivors (n = 49), 2.1 μmol/L (range: 0.5, 6.4, P < .001 versus controls) in nonsurvivors (n = 12) and 38.6 μmol/L (range: 11.4, 96.1) in controls (n = 14), respectively. There was no significant difference in plasma citrulline concentration between survivors and nonsurvivors within the parvovirus‐infected puppies, and plasma citrulline concentration was not significantly associated with outcome in parvoviral enteritis. There were no significant changes in plasma citrulline concentration over the 8‐day follow‐up period.Conclusion and Clinical Importance: Parvovirus enteritis is associated with a severe decrease in plasma citrulline concentration that does not appear to have any significant prognostic value.
Density, porosity, and magnetic susceptibility of achondritic meteorites
Authors: MACKE Robert J. , BRITT Daniel T. , CONSOLMAGNO Guy J. 
Published: January 2011

Abstract–As part of a large‐scale survey of meteorite bulk and grain densities, porosities, and magnetic susceptibilities, we measured these properties for 174 stones from 106 achondritic meteorites. These include four lunar meteorites, 15 stones from 10 shergottites, nakhlites, and chassignites (SNCs), 96 stones from 56 howardites, eucrites, and diogenites (HEDs), 17 stones from nine aubrites, two angrites, and 16 stones from 10 ureilites, four stones of three acapulcoites, as well as four stones of three lodranites, and 15 stones from eight primitive achondrites. Those meteorites derived from basalts and crustal material of differentiated parent bodies have lower densities and magnetic susceptibilities, on an average, than the more primitive achondrites, which have a higher percentage metal. A notable exception is the one chassignite in the study (Chassigny), which has a high grain density of 3.73 ± 0.04 g cm−3. Ureilites have magnetic susceptibilities consistent with primitive achondrites, but lower grain densities. Porosities do not vary considerably between most of the groups, with most stones 5–14% porous, although on an average, ureilites and brachinites have lower porosities, with most stones less than 7% porous. For primitive achondrites, the higher metal content causes finds to exhibit weathering effects similar to what is observed in ordinary chondrites, with a reduction in grain density, magnetic susceptibility, and porosity as compared with unweathered falls. For lunites, SNCs, and HEDs, no such effect is observed. We also observe that grain density and magnetic susceptibility used in conjunction distinguish shergottites, nakhlites, and chassignites from each other. Shergottites and nakhlites have low grain densities (averaging 3.31 and 3.41 g cm−3, respectively) whereas Chassigny is 3.7 g cm−3. In magnetic susceptibility, shergottities and chassignites are similar (averaging 2.85 and 2.98 in log units of 10−9 m3 kg−1, respectively) with nakhlites averaging higher at 3.42.
Novel VIM Metallo-β-Lactamase Variant, VIM-24, from a Klebsiella pneumoniae Isolate from Colombia
Authors: Montealegre Maria Camila , Correa Adriana , Briceño David F. , Rosas Natalia C. , De La Cadena Elsa , Ruiz Sory J. , Mojica Maria F. , Camargo Ruben Dario , Zuluaga Ivan , Marin Adriana , Quinn John P. , Villegas Maria Virginia ,   the Colombian Nosocomial Resistance Study Group
Published: January 2011

ABSTRACT

We report the emergence of a novel VIM variant (VIM-24) in a
Klebsiella pneumoniae
isolate in Colombia. The isolate displays MICs for carbapenems below the resistance breakpoints, posing a real challenge for its detection. The
bla
VIM-24
gene was located within a class 1 integron carried on a large plasmid. Further studies are needed to clarify its epidemiological and clinical impact.