bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vivo plant flow cytometry: A first proof‐of‐concept
Authors: Nedosekin Dmitry A. , Khodakovskaya Mariya V. , Biris Alexandru S. , Wang Daoyuan , Xu Yang , Villagarcia Hector , Galanzha Ekaterina I. , Zharov Vladimir P. 
Published: September 2011

AbstractIn vivo flow cytometry has facilitated advances in the ultrasensitive detection of tumor cells, bacteria, nanoparticles, dyes, and other normal and abnormal objects directly in blood and lymph circulatory systems. Here, we propose in vivo plant flow cytometry for the real‐time noninvasive study of nanomaterial transport in xylem and phloem plant vascular systems. As a proof of this concept, we demonstrate in vivo real‐time photoacoustic monitoring of quantum dot‐carbon nanotube conjugates uptake by roots and spreading through stem to leaves in a tomato plant. In addition, in vivo scanning cytometry using multimodal photoacoustic, photothermal, and fluorescent detection schematics provided multiplex detection and identification of nanoparticles accumulated in plant leaves in the presence of intensive absorption, scattering, and autofluorescent backgrounds. The use of a portable fiber‐based photoacoustic flow cytometer for studies of plant vasculature was demonstrated. These integrated cytometry modalities using both endogenous and exogenous contrast agents have a potential to open new avenues of in vivo study of the nutrients, products of photosynthesis and metabolism, nanoparticles, infectious agents, and other objects transported through plant vasculature. © 2011 International Society for Advancement of Cytometry
Induction of DNA damage signaling by oxidative stress in relation to DNA replication as detected using “click chemistry”
Authors: Zhao Hong , Dobrucki Jurek , Rybak Paulina , Traganos Frank , Dorota Halicka H. , Darzynkiewicz Zbigniew 
Published: September 2011

AbstractInduction of DNA damage by oxidants such as H2O2 activates the complex network of DNA damage response (DDR) pathways present in cells to initiate DNA repair, halt cell cycle progression, and prepare an apoptotic reaction. We have previously reported that activation of Ataxia Telangiectasia Mutated protein kinase (ATM) and induction of γH2AX are among the early events of the DDR induced by exposure of cells to H2O2, and in human pulmonary carcinoma A549 cells, both events were expressed predominantly during S‐phase. This study was designed to further explore a correlation between these events and DNA replication. Toward this end, we utilized 5‐ethynyl‐2′deoxyuridine (EdU) and the “click chemistry” approach to label DNA during replication, followed by exposure of A549 cells to H2O2. Multiparameter laser scanning cytometric analysis of these cells made it possible to identify DNA replicating cells and directly correlate H2O2‐induced ATM activation and induction of γH2AX with DNA replication on a cell by cell basis. After pulse‐labeling with EdU and exposure to H2O2, confocal microscopy was also used to examine the localization of DNA replication sites (“replication factories”) versus the H2AX phosphorylation sites (γH2AX foci) in nuclear chromatin in an attempt to observe the absence or presence of colocalization. The data indicate a close association between DNA replication and H2AX phosphorylation in A549 cells, suggesting that these DNA damage response events may be triggered by stalled replication forks and perhaps also by induction of DNA double‐strand breaks at the primary DNA lesions induced by H2O2. © 2011 International Society for Advancement of Cytometry.
Gal80 intersectional regulation of cell‐type specific expression in vertebrates
Authors: Fujimoto Esther , Gaynes Brooke , Brimley Cameron J. , Chien Chi‐Bin , Bonkowsky Joshua L. 
Published: September 2011

AbstractCharacterization and functional manipulation of specific groups of neurons in the vertebrate central nervous system (CNS) remains a major hurdle for understanding complex circuitry and functions. In zebrafish, the Gal4/UAS system has permitted expression of transgenes and enhancer trap screens, but is often limited by broad expression domains. We have developed a method for cell‐type specific expression using Gal80 inhibition of Gal4‐dependent expression. We show that native Gal4 is able to drive strong expression, that Gal80 can inhibit this expression, and that overlapping Gal4 and Gal80 expression can achieve “intersectional” expression in spatially and genetically defined subsets of neurons. We also optimize Gal80 for expression in vertebrates, track Gal80 expression with a co‐expressed fluorescent marker, and use a temperature‐sensitive allele of Gal80 to temporally regulate its function. These data demonstrate that Gal80 is a powerful addition to the genetic techniques available to map and manipulate neural circuits in zebrafish. Developmental Dynamics 240:2324–2334, 2011. © 2011 Wiley‐Liss, Inc.
The tight junction scaffolding protein cingulin regulates neural crest cell migration
Authors: Wu Chyong‐Yi , Jhingory Sharon , Taneyhill Lisa A. 
Published: September 2011

AbstractNeural crest cells give rise to a diverse range of structures during vertebrate development. These cells initially exist in the dorsal neuroepithelium and subsequently acquire the capacity to migrate. Although studies have documented the importance of adherens junctions in regulating neural crest cell migration, little attention has been paid to tight junctions during this process. We now identify the tight junction protein cingulin as a key regulator of neural crest migration. Cingulin knock‐down increases the migratory neural crest cell domain, which is correlated with a disruption of the neural tube basal lamina. Overexpression of cingulin also augments neural crest cell migration and is associated with similar basal lamina changes and an expansion of the premigratory neural crest population. Cingulin overexpression causes aberrant ventrolateral neuroepithelial cell delamination, which is linked to laminin loss and a decrease in RhoA. Together, our results highlight a novel function for cingulin in the neural crest. Developmental Dynamics 240:2309–2323, 2011. © 2011 Wiley‐Liss, Inc.
Summary of ‘Systemic antifungal therapy for tinea capitis in children’
Authors: Editorial Office Evidence‐Based Child Health 
Published: September 2011

AbstractThis is a summary of Cochrane review, published in this issue of EBCH, first published as: González U, Seaton T, Bergus G, Jacobson J, Martínez‐Monzón C. Systemic antifungal therapy for tinea capitis in children. Cochrane Database of Systematic Reviews 2007, Issue 4. Art. No.: CD004685. DOI: 10.1002/14651858. CD004685.pub2 Copyright © 2011 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. The Cochrane Collaboration
Commentary on ‘Systemic antifungal therapy for tinea capitis in children’
Authors: Fuller L. Claire 
Published: September 2011

AbstractThis is a Commentary of a Cochrane Review, published in this issue of EBCH, first published as: González U, Seaton T, Bergus G, Jacobson J, Martínez‐Monzón C. Systemic antifungal therapy for tinea capitis in children. Cochrane Database of Systematic Reviews 2007, Issue 4. Art. No.: CD004685. DOI: 10.1002/14651858.CD004685.pub2.Further information for this Cochrane review is available in this issue of EBCH in the accompanying Summary article. Copyright © 2011 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. The Cochrane Collaboration
Commentaries on ‘Minocycline for acne vulgaris: efficacy and safety’ with a response from the review authors
Authors: Del Rosso James Q. , Garner S.E. 
Published: September 2011

AbstractThese are commentaries of a Cochrane review, published in this issue of EBCH, first published as: Garner SE, Eady A, Popescu CM, Newton J, Li Wan Po A. Minocycline for acne vulgaris: efficacy and safety. Cochrane Database of Systematic Reviews 2003, Issue 1. Art. No.: CD002086. DOI: 10.1002/14651858.CD002086.Further information for this Cochrane review is available in this issue of EBCH in the accompanying Summary article. Copyright © 2011 The Cochrane Collaboration. Published by John Wiley & Sons, Ltd. The Cochrane Collaboration
Dye‐Sensitised Solar Cells Based on Large‐Pore Mesoporous TiO2 with Controllable Pore Diameters
Authors: Pan Kai , Zhou Wei , Tian Guohui , Pan Qingjiang , Tian Chungui , Xie Tengfeng , Dong Youzhen , Wang Dejun , Fu Honggang 
Published: September 2011

AbstractMesoporous TiO2 (meso‐TiO2) is a promising photoelectrical nanomaterial for high dye‐sensitised solar cell (DSSC) performance, because its nanochannels offer a large internal surface area to allow for more dye adsorption. The interconnected grains facilitate rapid electron transport both within the meso‐TiO2 film and at the film electrode dye/redox shuttle electrolyte interfaces. In this work, a series of DSSCs has been fabricated on the basis of meso‐TiO2 with large controllable pore sizes (6.5, 8.2 and 11.0 nm). It was found that the DSSC with the 8.2 nm meso‐TiO2 photoelectrode has the highest photoelectrical conversion efficiency, which is higher than that of the conventional P25 nanoparticulate DSSC under the same conditions. The measurements of the N2 sorption isotherms and the photovoltage transient demonstrates that such improved efficiency can be ascribed to the larger surface area and the fastest interfacial charge transfer. Meanwhile, the effect of different pore sizes on the photoelectrical conversion efficiency has been systematically investigated, and a possible model for the efficient electrolyte percolation in the dye‐sensitised mesoporous photoelectrode has been proposed.
A New Metal Carboxylate Framework Based on Porphyrin with Extended π‐Conjugation
Authors: Matsunaga Satoshi , Endo Nanako , Mori Wasuke 
Published: September 2011

AbstractWe have designed a new tetracarboxylporphyrin building block, ZnTCPEP‐H4, and used it in the construction of a novel porphyrin‐based metal carboxylate framework, [Zn4(μ3‐OH)2(H2O)2(ZnTCPEP‐H)2(DABCO)]·2DMF·10.5H2O(Zn4·ZnTCPEP·DABCO). The framework forms a 3D network linked by tetranuclear Zn cluster nodes {Zn4(μ3‐OH)2(H2O)2}, ZnTCPEP‐H4, and DABCO. The ZnTCPEP‐H4 building block exhibits extended π‐conjugation from the porphyrin core to the terminal carboxyl groups via ethynyl linkers attached to the porphyrin core. The Zn4·ZnTCPEP·DABCO framework gives rise to a 3D porous structure containing open channels with cross sections of approximately 10 × 10 and 7 × 7 Å. It has a BET surface area of 461 m2/g and a Langmuir surface area of 581 m2/g. A hydrogen adsorption capacity of 0.86 wt.‐% was determined for Zn4·ZnTCPEP·DABCO at 77 K and 0.1 MPa.
Aqueous–Organic Biphasic Hydrogenation of trans‐Cinnamaldehyde Catalyzed by Rhodium and Ruthenium Phosphane‐Free Porphyrin Complexes
Authors: Stangel Christina , Charalambidis Georgios , Varda Varvara , Coutsolelos Athanassios G. , Kostas Ioannis D. 
Published: September 2011

AbstractThe synthesis and spectroscopic characterization of new rhodium and ruthenium complexes with a porphyrin, possessing carboxylic groups in the arms of the porphyrin ring, is described. The complexes are completely soluble in water in the presence of a base (KOH or triethylamine). These phosphane‐free and air‐stable complexes were evaluated as catalysts in the aqueous hydrogenation of trans‐cinnamaldehyde. Catalysis was more efficient with the ammonium salts of the complexes rather than the corresponding potassium carboxylate salts, preferably in water/toluene rather than in neat water. The effect of catalyst concentration, reaction temperature, hydrogen pressure and methanol addition on the catalytic activity of the complexes in the presence of triethylamine in an aqueous–organic biphasic system was investigated. The catalysts were presumably homogeneous, and could be recycled and reused without any significant difference in activity and selectivity. This is the first time that porphyrin–metal complexes have been used as catalysts for hydrogenation of α,β‐unsaturated aldehydes.
Chemical modification of proteins to improve the accuracy of their relative molecular mass determination by electrophoresis
Authors: Dolnik Vladislav , Gurske William A. 
Published: September 2011

AbstractWe studied the electrophoretic behavior of basic proteins (cytochrome c and histone III) and developed a carbamylation method that normalizes their electrophoretic size separation and improves the accuracy of their relative molecular mass determined electrophoretically. In capillary zone electrophoresis with cationic hitchhiking, native cytochrome c does not sufficiently bind cationic surfactants due to electrostatic repulsion between the basic protein and cationic surfactant. Carbamylation suppresses the strong positive charge of the basic proteins and results in more accurate relative molecular masses.
In vitroevaluation of an injectable chitosan gel for sustained local delivery of BMP‐2 for osteoblastic differentiation
Authors: Kim Sungwoo , Tsao Helen , Kang Yunqing , Young Daniel A. , Sen Milan , Wenke Joseph C. , Yang Yunzhi 
Published: September 2011

AbstractWe investigated the effect of sustained release of bone morphogenetic protein‐2 (BMP‐2) from an injectable chitosan gel on osteoblastic differentiationin vitro. We first characterized the release profile of BMP‐2 from the gels, and then examined the cellular responses of preosteoblast mouse stromal cells (W‐20‐17) and human embryonic palatal mesenchymal (HEPM) cells to BMP‐2. The release profiles of different concentrations of BMP‐2 exhibited sustained releases (41% for 2 ng/mL and 48% for 20 ng/mL, respectively) from the chitosan gels over a three‐week period. Both cell types cultured in the chitosan gels were viable and significantly proliferated for 3 days (p< 0.05). Chitosan gels loaded with BMP‐2 enhanced ALP activity of W‐20‐17 by 3.6‐fold, and increased calcium mineral deposition of HEPM by 2.8‐fold at 14 days of incubation, compared to control groups initially containing the same amount of BMP‐2. In addition, schitosan gels loaded with BMP‐2 exhibited significantly greater osteocalcin synthesis of W‐20‐17 at seven days, and of HEPM at both 7 and 14 days compared with the control groups (p<0.05). This study suggests that the enhanced effects of BMP‐2 released from chitosan gels on cell differentiation and mineralization are species and cell type dependent. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.
A simplified in vivo approach for evaluating the bioabsorbable behavior of candidate stent materials
Authors: Pierson Daniel , Edick Jacob , Tauscher Aaron , Pokorney Ellen , Bowen Patrick , Gelbaugh Jesse , Stinson Jon , Getty Heather , Lee Chee Huei , Drelich Jaroslaw , Goldman Jeremy 
Published: September 2011

AbstractMetal stents are commonly used to revascularize occluded arteries. A bioabsorbable metal stent that harmlessly erodes away over time may minimize the normal chronic risks associated with permanent implants. However, there is no simple, low‐cost method of introducing candidate materials into the arterial environment. Here, we developed a novel experimental model where a biomaterial wire is implanted into a rat artery lumen (simulating bioabsorbable stent blood contact) or artery wall (simulating bioabsorbable stent matrix contact). We use this model to clarify the corrosion mechanism of iron (≥99.5 wt %), which is a candidate bioabsorbable stent material due to its biocompatibility and mechanical strength. We found that iron wire encapsulation within the arterial wall extracellular matrix resulted in substantial biocorrosion by 22 days, with a voluminous corrosion product retained within the vessel wall at 9 months. In contrast, the blood‐contacting luminal implant experienced minimal biocorrosion at 9 months. The importance of arterial blood versus arterial wall contact for regulating biocorrosion was confirmed with magnesium wires. We found that magnesium was highly corroded when placed in the arterial wall but was not corroded when exposed to blood in the arterial lumen for 3 weeks. The results demonstrate the capability of the vascular implantation model to conduct rapid in vivo assessments of vascular biomaterial corrosion behavior and to predict long‐term biocorrosion behavior from material analyses. The results also highlight the critical role of the arterial environment (blood vs. matrix contact) in directing the corrosion behavior of biodegradable metals. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
Optimizing conditions for the extraction of catechins from green tea using hot water
Authors: Vuong Quan V. , Golding John B. , Stathopoulos Costas E. , Nguyen Minh H. , Roach Paul D. 
Published: September 2011

AbstractSix different factors involved in the extraction of catechins from green tea using water were examined for their impact on the yield of catechins and on the efficiency of water use. The best temperature and time combination for catechin extraction was at 80°C for 30 min. The yield of catechins was also optimal with a tea particle size of 1 mm, a brewing solution pH <6 and a tea‐to‐water ratio at 50:1 (mL/g). In terms of efficient use of water in a single extraction, a water‐to‐tea ratio of 20:1 (mL/g) gave the best results; 2.5 times less water was used per gram of green tea. At the water‐to‐tea ratio of 20:1 mL/g, the highest yield of catechins per gram of green tea was achieved by extracting the same sample of green tea twice. However, for the most efficient use of water, the best extraction was found to be once at a water‐to‐tea ratio of 12:1 (mL/g) and once at a water‐to‐tea ratio of 8:1 (mL/g). Therefore, all six of the factors investigated had an impact on the yield of catechins extracted from green tea using water and two had an impact on the efficiency of water use.
Emulsion Electrospinning of a Collagen‐Like Protein/PLGA Fibrous Scaffold: Empirical Modeling and Preliminary Release Assessment of Encapsulated Protein
Authors: Wei Kai , Li Yuan , Lei Xiaowen , Yang Heejae , Teramoto Akira , Yao Juming , Abe Koji , Ko Frank K. 
Published: September 2011

AbstractThe effectiveness of a multifunctional scaffold produced by the electrospinning of emulsions composed of organic PLGA and aqueous collagen‐like protein (denoted as Fol‐8Col) solutions is demonstrated. The resultant Fol‐8Col/PLGA fibrous scaffolds with homogeneous morphology have mean fiber diameters from 600 to 2 000 nm. A uniform distribution of encapsulated Fol‐8Col in the fibers is observed by fluorescence microscopy. TEM is used to clarify the representative core/sheath structure of emulsion electrospun Fol‐8Col/PLGA fibers. Preliminary release assessment of encapsulated Fol‐8Col shows results of sustained release for more than one month from the Fol‐8Col/PLGA fibrous mats. The cytocompatibility of fibroblast cell line L929 with the fibrous composite seems promosing. magnified image
Rectangular‐Shaped Polyaniline Tubes Covered with Nanorods and their Electrorheology
Authors: Lee Bo Mi , Kim Ji Eun , Fang Fei Fei , Choi Hyoung Jin , Feller Jean‐Francois 
Published: September 2011

AbstractNanorod covered polyaniline (PANI) tubes with rectangular cross section are synthesized via a chemical oxidation polymerization method and then utilized as an electrorheological (ER) material by dispersion in silicone oil, resulting in 10 vol% particle concentration. These PANI tubes are chosen based on the fact that the PANI is a promising suspension material for ER fluids due to its high polarizability and controllable conductivity. The ER performances are investigated as a function of electric field strength by using a rheometer, and the frequency dependences of permittivity and dielectric loss factor of ER fluids are also measured with a LCR meter. The sedimentation observation is further examined as a function of time, demonstrating improved dispersion stability compared to general particulate PANI‐based ER fluid.
Mimic of Protein: A Highly pH‐Sensitive and Thermoresponsive Polyampholyte
Authors: Xia Yuqiong , Gao Min , Chen Ye , Jia Xinru , Liang Dehai 
Published: September 2011

AbstractAs a mimic of protein, a polyampholyte‐based material should be able to duplicate the properties and functions of protein. A polyampholyte that is highly sensitive to both pH and temperature under physiological conditions is obtained when 80% butylamide‐terminated poly(amidoamine) dentron is grafted to the backbone of styrene and maleic anhydride. The phase separation occurs at 33.7 °C at pH = 6.20, while the transition point increases to 43.9 °C at pH = 6.30. The superior performance is the consequence of the cooperative interactions among basic, acidic, and thermoresponsive groups. The polyampholyte with a suitable isoelectric point provides a platform for the development of multifunctional materials for biomedical applications.
Impact experiments and finite element simulation of surface roughness reduction by machine hammer peening
Authors: Wied J. , Scheil J. , Klamser M. , Berger C. 
Published: September 2011

AbstractMachine hammer peening (MHP) is a mechanical surface treatment that employs a tungsten carbide tool to hammer the surface of a work piece. It was the goal of this study to find relations between the process parameters and the surface smoothing effect of MHP by finite element simulations. Impact experiments have been conducted in order to find suitable load‐deformation‐relations for a selection of work piece materials and to validate the model. A finite element model has been established with good agreement to the experimental results. Simulations have been conducted to investigate the influence of main process parameters on the surface roughness reduction. Additional simulations were performed and a way of creating uniform smooth surfaces with respect to small duration of surface treatment is presented.
Highly loaded alumina inks for use in a piezoelectric print head
Authors: Polsakiewicz D. A. , Kollenberg W. 
Published: September 2011

AbstractInk jet printing techniques are used for an increasing number of materials and applications. Properties of the used inks have great influence on process stability and accuracy as well as the resulting part properties. Inks containing ceramic particles have been applied in various studies and applications. The criteria identifying suitable inks vary depending on the applied printing technology. In this study, inks for application in a piezoelectric print head were investigated. Alumina inks with solid loadings up to 21 vol.‐% were prepared and characterized. The Influence of different solid loadings, dispersant concentrations and binding agents was examined. The prepared inks show sufficient stability and fit literature recommendations for successful drop ejection. Considering rheological aspects, two inks with light shear‐thinning behaviour and viscosities of 18 mPas and 23 mPas at high shear rates were recommended for upcoming printing tests. The Re/We1/2 ratio can be used for predetermination of print ability but the influence of the surface tension is not sufficiently taken into account using the presented calculations. So further printing experiments are essential for clarification of the calculations and obtained print ability values.
Investigation concerning the applicability of raman spectroscopy for prospective inline monitoring of electrode processing for lithium ion batteries
Authors: Langklotz U. , Schneider M. , Seifert C. , Michaelis A. 
Published: September 2011

AbstractRaman spectroscopy was used for investigation of tape‐cast LCO foils. The aim of the work was to test the suitability of a portable and cost‐effective Raman spectrometer as a tool for inline monitoring of electrode processing. The influence of device‐specific limitations, as well as intrinsic material properties, of LCO electrodes was investigated. It could be shown that the heat impact caused by laser excitation is negligible. Typical electrode properties as roughness or orientation do not significantly the Raman measurements. Based on this investigation, Raman spectroscopy is a promising tool for intended inline monitoring.