bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prognostic factors for the two‐year course of activity limitations in early osteoarthritis of the hip and/or knee
Authors: Holla Jasmijn F. M. , Steultjens Martijn P. M. , Roorda Leo D. , Heymans Martijn W. , ten Wolde Saskia , Dekker Joost 
Published: May 2010

AbstractObjectiveTo predict the 2‐year course of activity limitations in patients with early knee and/or hip osteoarthritis (OA).MethodsThe Cohort Hip & Cohort Knee (CHECK) study is a prospective followup study. The CHECK cohort, comprising participants (n = 1,002) with early OA‐related knee and/or hip symptoms, was followed for 2 years. Participants completed questionnaires and underwent physical, laboratory, and radiographic examination. Regression models were used to examine whether baseline variables predicted the course of activity limitations as measured with the Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC). Analyses were performed separately for participants with knee symptoms and participants with hip symptoms.ResultsAfter 2 years of followup, activity limitations slightly decreased. Large between‐subject variation was observed in WOMAC change scores. In participants with knee symptoms, young age, non‐Western ethnicity, bilateral hip pain, morning stiffness in the knee, high comorbidity count, high body mass index, high bodily pain, poor general health perception, and pain coping strategy were associated with a poor 2‐year outcome on activity limitations. In participants with hip symptoms, few activity limitations at baseline, bilateral hip pain, morning stiffness in the knee, high comorbidity count, low active hip flexion, poor general health perception, and pain coping strategy were associated with a poor 2‐year outcome on activity limitations.ConclusionAfter 2 years of followup, large between‐subject variation was observed in the course of activity limitations. The course of activity limitations is to some extent already predictable at an early stage of knee and hip OA.
Therapeutic Window for Bioactive Nanocomposites Fabricated by Laser Ablation in Polymer‐Doped Organic Liquids
Authors: Hahn Anne , Stöver Timo , Paasche Gerrit , Löbler Marian , Sternberg Katrin , Rohm Henning , Barcikowski Stephan 
Published: May 2010

AbstractPolymeric nanomaterials are gaining increased interest in medical applications due to the sustained release of bioactive agents. Within this study nanomaterials are fabricated using laser ablation of silver and copper in polymer‐doped organic liquids thus allowing to produce customized drug release systems. A strategy is shown to determine the therapeutic window for cells relevant for cochlear implant electrodes, defined by the viability of L929 fibroblasts, PC12 neuronal cells, and spiral ganglion cells on different concentrations of silver and copper ions. The distribution of nanoparticles within the silicone polymer matrix is determined using transmission electron microscopy. Hexane doped with 1% silicone resin is found to be an appropriate liquid matrix to fabricate a nanocomposite with a constant ion release rate. Silver ions of 10 µmol L−1 or copper ions of 100 µmol L−1 cause a suppression of tissue growth without inhibiting neuronal cell growth. The copper nanoparticle content of 0.1 wt% of the silicone composite releases ion concentrations which fit the therapeutic window.
Heck reaction catalyzed by a recyclable palladium supported on shell powder
Authors: Shen Yong‐Miao , Du Yi‐Jun , Zeng Min‐Feng , Zhi Duo , Zhao Sheng‐Xian , Rong Lin‐Mei , Lv Shao‐Qin , Du Lin , Qi Chen‐Ze 
Published: May 2010

AbstractA novel palladium catalyst supported on shell powder has been prepared, and its application to the Heck reaction of aryl iodides with olefins has been reported. The results showed that the novel catalyst had extremely high activities for the reactions with the average yield over 90%. Also, this catalyst showed excellent stability in Heck reactions, being reused three times. The catalyst was characterized by X‐ray powder diffraction and field‐emission scanned electron microscopy images, and the energy dispersive X‐ray analyzer. Copyright © 2010 John Wiley & Sons, Ltd.
Human articular chondrocytes secrete parathyroid hormone–related protein and inhibit hypertrophy of mesenchymal stem cells in coculture during chondrogenesis
Authors: Fischer J. , Dickhut A. , Rickert M. , Richter W. 
Published: May 2010

AbstractObjectiveThe use of bone marrow–derived mesenchymal stem cells (MSCs) has shown promise in cell‐based cartilage regeneration. A yet‐unsolved problem, however, is the unwanted up‐regulation of markers of hypertrophy, such as alkaline phosphatase (AP) and type X collagen, during in vitro chondrogenesis and the formation of unstable calcifying cartilage at heterotopic sites. In contrast, articular chondrocytes produce stable, nonmineralizing cartilage. The aim of this study was to address whether coculture of MSCs with human articular chondrocytes (HACs) can suppress the undesired hypertrophy in differentiating MSCs.MethodsMSCs were differentiated in chondrogenic medium that had or had not been conditioned by parallel culture with HAC pellets, or MSCs were mixed in the same pellet with the HACs (1:1 or 1:2 ratio) and cultured for 6 weeks. Following in vitro differentiation, the pellets were transplanted into SCID mice.ResultsThe gene expression ratio of COL10A1 to COL2A1 and of Indian hedgehog (IHH) to COL2A1 was significantly reduced by differentiation in HAC‐conditioned medium, and less type X collagen protein was deposited relative to type II collagen. AP activity was significantly lower (P < 0.05) in the cells that had been differentiated in conditioned medium, and transplants showed significantly reduced calcification in vivo. In mixed HAC/MSC pellets, suppression of AP was dose‐dependent, and in vivo calcification was fully inhibited. Chondrocytes secreted parathyroid hormone–related protein (PTHrP) throughout the culture period, whereas PTHrP was down‐regulated in favor of IHH up‐regulation in control MSCs after 2–3 weeks of chondrogenesis. The main inhibitory effects seen with HAC‐conditioned medium were reproducible by PTHrP supplementation of unconditioned medium.ConclusionHAC‐derived soluble factors and direct coculture are potent means of improving chondrogenesis and suppressing the hypertrophic development of MSCs. PTHrP is an important candidate soluble factor involved in this effect.
Mortality in Behçet's disease
Authors: Saadoun D. , Wechsler B. , Desseaux K. , Huong D. Le Thi , Amoura Z. , Resche‐Rigon M. , Cacoub P. 
Published: May 2010

AbstractObjectiveTo report the long‐term mortality in patients with Behçet's disease (BD).MethodsA cohort of 817 patients fulfilling the international criteria for BD from a single center in France were analyzed for causes of death, the standardized mortality ratio (SMR), and the factors associated with mortality.ResultsAmong the 817 patients with BD, 41 (5%) died after a median followup of 7.7 years, of whom 95.1% were male. The mean ± SD age at death was 34.8 ± 11.9 years. Main causes of death included major vessel disease (mainly, arterial aneurysm and Budd‐Chiari syndrome) (43.9%), cancer and malignant hemopathy (14.6%), central nervous system involvement (12.2%), and sepsis (12.2%). The mortality rate at 1 year and 5 years was 1.2% and 3.3%, respectively. There was an increased mortality among patients ages 15–24 years (SMR 2.99, 95% confidence interval [95% CI] 1.54–5.39) and those ages 25–34 years (SMR 2.90, 95% CI 1.80–4.49) as compared with age‐and sex‐matched healthy controls. The mortality decreased in patients older than age 35 years (SMR 1.23, 95% CI 0.75–1.92). In multivariate analyses, male sex (hazard ratio [HR] 4.94, 95% CI 1.53–16.43), arterial involvement (HR 2.51, 95% CI 1.07–5.90), and a high number of BD flares (HR 2.37, 95% CI 1.09–5.14) were independently associated with the risk of mortality.ConclusionThe overall mortality in our BD cohort was 5% after a median followup of 7.7 years. Male sex, arterial involvement, and the number of flares were associated with mortality in BD.
Autoimmunity to peptidyl arginine deiminase type 4 precedes clinical onset of rheumatoid arthritis
Authors: Kolfenbach Jason R. , Deane Kevin D. , Derber Lezlie A. , O'Donnell Colin I. , Gilliland William R. , Edison Jess D. , Rosen Antony , Darrah Erika , Norris Jill M. , Holers V. Michael 
Published: May 2010

AbstractObjectiveTo determine whether antibodies against peptidyl arginine deiminase type 4 (PAD‐4) are present in the preclinical phase of rheumatoid arthritis (RA) and to compare the timing and extent of their appearance with those of other preclinical autoantibodies.MethodsPrediagnosis serum samples from 83 patients with RA were evaluated for the presence of anti–PAD‐4 antibody, anti–cyclic citrullinated peptide (anti‐CCP) antibody, and rheumatoid factor. In addition, a control cohort (n = 83) matched by age, sex, race, number of serum samples, and duration of serum storage was tested for the presence of anti–PAD‐4 antibody to determine its sensitivity and specificity for the subsequent development of RA.ResultsFifteen of 83 patients with RA (18.1%) had at least 1 prediagnosis sample positive for anti–PAD‐4. One of 83 control subjects (1.2%) had at least 1 positive sample, resulting in a sensitivity and specificity of 18.1% and 98.8%, respectively, of anti–PAD‐4 for the future development of RA. The mean duration of anti–PAD‐4 positivity prior to clinical diagnosis was 4.67 years. Anti–PAD‐4 positivity was associated with anti‐CCP positivity (odds ratio 5.13 [95% confidence interval 1.07–24.5]). In subjects with prediagnosis samples that were positive for both antibodies, anti‐CCP positivity predated anti–PAD‐4 positivity in 9 of 13 cases (69%).ConclusionAutoantibodies to PAD‐4 are present during the preclinical phase of RA in a subset of patients and are associated with anti‐CCP positivity. Further exploration is needed regarding the timing of appearance and disease‐related effects of PAD‐4 autoimmunity.
A novel autoantibody recognizing 200‐kd and 100‐kd proteins is associated with an immune‐mediated necrotizing myopathy
Authors: Christopher‐Stine Lisa , Casciola‐Rosen Livia A. , Hong Grace , Chung Tae , Corse Andrea M. , Mammen Andrew L. 
Published: May 2010

AbstractObjectiveMyofiber necrosis without prominent inflammation is a nonspecific finding in patients with dystrophies and toxic or immune‐mediated myopathies. However, the etiology of a necrotizing myopathy is often obscure, and the question of which patients would benefit from immunosuppression remains unanswered. The aim of this study was to identify novel autoantibodies in patients with necrotizing myopathy.MethodsMuscle biopsy specimens and serum samples were available for 225 patients with myopathy. Antibody specificities were determined by performing immunoprecipitations from 35S‐methionine–labeled HeLa cell lysates. Selected biopsy specimens were stained for membrane attack complex, class I major histocompatibility complex (MHC), and endothelial cell marker CD31.ResultsMuscle biopsy specimens from 38 of 225 patients showed predominantly myofiber necrosis. Twelve of these patients had a known autoantibody association with or other etiology for their myopathy. Sixteen of the remaining 26 sera immunoprecipitated 200‐kd and 100‐kd proteins; this specificity was observed in only 1 of 187 patients without necrotizing myopathy. Patients with the anti‐200/100 autoantibody specificity had proximal weakness (100%), high creatine kinase levels (mean maximum 10,333 IU/liter), and an irritable myopathy on electromyography (88%). Sixty‐three percent of these patients had been exposed to statins prior to the onset of weakness. All patients responded to immunosuppressive therapy, and many experienced a relapse of weakness when the medication was tapered. Immunohistochemical studies showed membrane attack complex on small blood vessels in 6 of 8 patients and on the surface of non‐necrotic myofibers in 4 of 8 patients. Five of 8 patients had abnormal capillary morphology, and 4 of 8 patients expressed class I MHC on the surface of non‐necrotic myofibers.ConclusionAn anti–200/100‐kd specificity defines a subgroup of patients with necrotizing myopathy who previously were considered to be autoantibody negative. We propose that these patients have an immune‐mediated myopathy that is frequently associated with prior statin use and should be treated with immunosuppressive therapy.
Modelling passengers, buses and stops in traffic microsimulation: review and extensions
Authors: Cortés Cristián E. , Burgos Vanessa , Fernández Rodrigo 
Published: May 2010

AbstractIn the last decade, significant research efforts and technology have been dedicated to the development of microsimulation tools for a better representation of traffic systems. As a result, several commercial packages appeared and they are used nowadays in the detailed modelling of different transportation systems and operations for specific project evaluations and local designs, mostly within the urban context. After reviewing the specialized literature, we realized that most of these microsimulation tools are oriented to the movement of cars, leaving the public transportation systems as a complement, just for a realistic representation of the transportation system as a whole, but always oriented to simulate cars. In this paper, the objective is to provide guidelines on how to incorporate the necessary entities and components for a proper simulation of public transport systems in a microsimulation environment. Thus, the different approaches to simulate transit systems at a microlevel are discussed, highlighting the necessity of including stops, passengers and transit vehicles explicitly as entities within the microsimulation environment, for modelling transfer operations, control strategies, etc. Several examples are then provided to quantify the impact of such representations, for different cases and potential simulation platforms. Copyright © 2010 John Wiley & Sons, Ltd.
The right tool for the job? Comparing an evidence accumulation and a naive strategy selection model of decision making
Authors: Newell Ben R. , Lee Michael D. 
Published: May 2010

AbstractAnalyses of multi‐attribute decision problems are dominated by accounts which assume people select from a repertoire of cognitive strategies to make decisions. This paper explores an alternative account based on sequential sampling and evidence accumulation. Two experiments varied aspects of a decision environment to examine competing models of decision behavior. The results highlighted the intra‐participant consistency but inter‐participant differences in the amount of evidence considered in decisions. This pattern was best captured by a sequential evidence accumulation model (SEQ) which treated pure Take‐The‐Best (TTB) and pure “rational” (RAT) models as special cases of a single model. The SEQ model was also preferred by the minimum description length (MDL) criterion to a naive strategy‐selection model (NSS) which assumed that TTB or RAT could be selected with some probability for each decision. Copyright © 2010 John Wiley & Sons, Ltd.
Spatial‐temporal patterns and pedestrian simulation
Authors: Hu Nan , Zhou Suiping , Wu Zhongke , Zhou Mingquan , Cho Benjamin Eng Keong 
Published: May 2010

AbstractIn this paper, we propose a framework for modeling lower‐level pedestrian navigational behaviors. We aim not only to generate realistic simulation results but also to make our framework flexible and extendible, and easy to use for model developers. A divide‐and‐conquer methodology is first adopted to divide the complex navigational behaviors into three levels, which allows us to focus on the intermediate level. We then propose a pattern‐based framework for modeling pedestrian navigational behavior at this level. In our framework, spatial‐temporal patterns are used to represent the situational perception, and a pattern‐matching mechanism is proposed to model the navigational choices of individual pedestrians. To demonstrate the effectiveness of our framework, a computational model is constructed to simulate pedestrian behaviors in a corridor with medium to relatively high density of pedestrians. Simulation results with this model are summarized in this paper. Copyright © 2010 John Wiley & Sons, Ltd.
Simple and parallel proximity algorithms for general polygonal models
Authors: Lee Youngeun , Kim Young J. 
Published: May 2010

AbstractWe present simple and fast parallel proximity algorithms for rigid polygonal models. Given two polygon‐soup models in space, if they overlap, our algorithm can find all the intersected primitives between them; otherwise, it reports their Euclidean minimum distance. Our algorithm is performed in a parallel fashion and shows scalable performance in terms of the number of available computing cores. The key ingredient of our algorithm is a simple load‐balancing metric based on the penetration depth (PD) (for collision detection) and approximate Euclidean distance (for Euclidean distance computation) between bounding volumes. To compute the PD between oriented bounding boxes (OBBs), we present a novel algorithm based on the well‐known separating axis theorem (SAT) and also shows that the PD can be trivially obtained as a byproduct of SAT. We have implemented these algorithms on a commodity PC with eight cores and benchmarked their performance on complicated geometric models. In practice, the performance of our algorithm shows up to 5 and 9.7 times improvement for collision and distance queries, respectively, compared to single core computation. Copyright © 2010 John Wiley & Sons, Ltd.
Lymphocytes transfer [14C]‐labeled fatty acids to skeletal muscle in culture; modulation by exercise
Authors: Brito Gleisson A. P. , Nunes Everson A. , Nogata Cláudia , Yamazaky Ricardo K. , Naliwaiko Katya , Curi Rui , Fernandes Luiz C. 
Published: May 2010

AbstractPrevious studies have shown that lipids are transferred from lymphocytes (Ly) to different cell types including macrophages, enterocytes, and pancreatic β cells in co‐culture. This study investigated whether [14C]‐labeled fatty acids (FA) can be transferred from Ly to skeletal muscle (SM), and the effects of exercise on such phenomenon. Ly obtained from exercised (EX) and control (C) male Wistar rats were preloaded with the [14C]‐labeled free FA palmitic (PA), oleic (OA), linoleic (LA), or arachidonic (AA). Radioactively loaded Ly were then co‐cultured with SM from the same Ly donor animals. Substantial amounts of FA were transferred to SM being the profile PA = OA > AA > LA to the C group, and PA > OA > LA > AA to the EX group. These FA were incorporated predominantly as phospholipids (PA = 66.75%; OA = 63.09%; LA = 43.86%; AA = 47.40%) in the C group and (PA = 63.99% OA = 52.72%; LA = 55.99%; AA = 63.40%) in the EX group. Also in this group, the remaining radioactivity from AA, LA, and OA acids was mainly incorporated in structural and energetic lipids. These results support the hypothesis that Ly are able to export lipids to SM in co‐culture. Furthermore, exercise modulates the lipid transference profile, and its incorporation on SM. The overall significance of this phenomenon in vivo remains to be elucidated. Copyright © 2010 John Wiley & Sons, Ltd.
Identification of heterogeneous nuclear ribonucleoprotein A/B as a cytoplasmic mRNA‐binding protein in early involution of the mouse mammary gland
Authors: Taga Yuki , Miyoshi Masaya , Okajima Tetsuya , Matsuda Tsukasa , Nadano Daita 
Published: May 2010

AbstractInvolution of the mammary gland is a regressive phase that occurs after lactation, and requires reprogramming of gene expression for the tissue to return to a pre‐pregnant state. Although the transcriptome of the mammary gland demonstrates complex changes at the mRNA level, the molecular mechanisms governing post‐transcriptional control remain obscure. In the present study, we isolated cytoplasmic mRNA‐protein complexes (mRNPs) from the mouse mammary gland at the early involution stage using discontinuous sucrose density ultracentrifugation. mRNPs including untranslated mRNAs were then purified with oligo(dT) immobilized on cellulose or paramagnetic beads. Proteins in the purified complexes were subjected to one/two‐dimensional gel electrophoresis followed by mass spectrometry. This identified heterogeneous nuclear ribonucleoprotein A/B (Hnrpab), along with three other heterogeneous nuclear ribonucleoproteins. Hnrpab in the mRNPs reproducibly increased within 48 h after weaning and became one of the major components. When a vector expressing Hnrpab was transfected into two different cell lines, their growth was suppressed, demonstrating that this protein has cytostatic activity. These results suggest that early involution can be used as a model for understanding the mechanism of post‐transcriptional control of gene expression, responsible for modulation of cell function. Copyright © 2010 John Wiley & Sons, Ltd.
ChemInform Abstract: High Pressure Behavior of Mercury Cyanamide HgCN2.
Authors: Liu Haozhe , Klein Wilhelm , Bender Holger , Jansen Martin 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Electrochemical Li Insertion in B‐Doped Multiwall Carbon Nanotubes.
Authors: Mukhopadhyay I. , Hoshino N. , Kawasaki S. , Okino F. , Hsu W. K. , Touhara H. 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Thiosulfonates with Functional Groups in the Aliphatic Chain.
Authors: Baranovich D. B. , Lubenets V. I. , Novikov V. P. 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: 1,2,5‐Oxadiazole N‐Oxide Derivatives as Potential Anticancer Agents: Synthesis and Biological Evaluation. Part 4.
Authors: Boiani Mariana , Cerecetto Hugo , Gonzalez Mercedes , Risso Mariela , Olea‐Azar Claudio , Piro Oscar E. , Castellano Eduardo E. , Lopez de Cerain Adela , Ezpeleta Olga , Monge‐Vega Antonio 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The Synthesis of Isochroman‐4‐ols and Isochroman‐3‐ols: Models for Naturally Occurring Benzo[g]isochromanols.
Authors: de Koning Charles B. , Green Ivan R. , Michael Joseph P. , Oliveira Jose R. 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Preparation of the Enantiomers of 1‐Phenylethan‐1,2‐diol. Regio‐ and Enantioselectivity of Acylase I and Candida antarctica Lipases A and B.
Authors: Virsu Pauliina , Liljeblad Arto , Kanerva Anu , Kanerva Liisa T. 
Published: May 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.