bluuebunny/crossref_metadata_2025_split huggingface dataset:
ISOLASI DAN IDENTIFIKASI MORFOLOGI SERTA UJI PELARUTAN FOSFAT TERHADAP BAKTERI RHIZOSFER TANAMAN KEDELAI (Glycine max L.)
Authors: Adriantama Syahreza , Suriyanti Suriyanti , Nontji Maimuna 
Published: June 2024

Isolation and Morphological Identification and Phosphate Dissolution Test against Rhizosphere Bacteria in Soybean (Glycine max L.). Supervised by Suriyanti, and Maimuna Nontji. This research was conducted to obtain bacterial isolates from the results of isolation and morphological identification of Rhizosphere bacteria in soybean plants, to obtain bacterial isolates capable of dissolving phosphates and calculating the clear zones of bacterial isolates. The research was carried out at the Microbiology Laboratory, Faculty of Pharmaceutical Sciences and at the Pest Laboratory and The Faculty of Agriculture, Muslim University of Indonesia will take place from December 2019 to February 2020. The materials to be used are heat-resistant plastic, aluminum foil, plastic wrap for soil samples of soybean Rhizosphere, nutrient agar (NA) media, phykovskaya media, yeast media. extract mannitol agar (YEMA), congo red (CR), bromine thymol blue (BTB) and 1000 ml distilled water.The results of isolation and identification of bacterial isolates from the rhizosphere of soybean plants (Glycine Max.L) were obtained as many as 23 isolates with various characteristics, almost all of which were milky white and soft in consistency by testing 8 isolates from 23 isolates with phycovkaya media showing 2 isolates were able to dissolve phosphate with an average Phosphate Solvent Index for isolate 13 of 1.5 and isolate 20 of 0.75. By testing on YEMA media with both Congo Red and Brom thymol blue indicators, no isolates were identified as Rhizobium bacteria due to contamination.
RESPON PERTUMBUHAN DAN PRODUKSI TANAMAN UBI JALAR (Ipomoea batatas L.) TERHADAP PEMBERIAN ARANG SEKAM
Authors: Akhmad Akhmad , Saida Saida , Nontji Maimuna 
Published: June 2024

AKHMAD (08220160081)Response of growth and production of sweet potato to rice husk charcoal This research was conducted in Tanah Towa village of Kajang sub-district, Bulukumba district starting from December 2020 to February 2021. This research is in the form of an experiment that was carried out in a bed by giving cekan charcoal, the layout of the research in the field was arranged based on a randomized block design (RAK) with 3 replications. The treatment design was carried out with a one-factor factorial design with 5 different treatments, namely the provision of husk charcoal 0 kg per plant, 0.25 kg per plant, 0.50 kg per plant, 0.75 kg per plant and 1 kg per plant for each treatment in repeated three times so that there are 15 experimental units. The results showed that the administration of husk charcoal 0.59 kg per plant gave the best effect in increasing the number of tubers per plant, the number of tubers per plot and the weight of the tubers in the pet plot.
TEKNOLOGI MUTASI GEN UNTUK MENINGKATKAN KERAGAMAN GENETIK BEBERAPA VARIETAS KEDELAI (Glycine max L) YANG BERUMUR GENJAH DAN TOLERAN TERHADAP CEKAMAN AIR
Authors: Nurhidayat Nurhidayat , Aminah Aminah , Edy Edy 
Published: June 2024

This study aimed to know the effect of several gene mutations on the growth and production of soybean plants, effect of water stress (drought stress and water saturation stress) on the growth and production of soybeans, interaction between Soybean varieties resulting from gene mutations and water stress on the growth and production of soybeans. The research was carried out in the experimental garden of the Soil Laboratory of the Agricultural Technology Research Center, Maros Regency, which took place from June to September 2021. This study used a split plot design method, where the main plot was water stress, which consisted of two levels, namely stress stress. Drought and water saturation stress and their subplots were soybean varieties resulting from mutations in the first gene, which consisted of three, namely Anjasmoro, Argomulyo and Dena 1, so that six treatment combinations were obtained and repeated three times to obtain 18 experimental units. The results of the study showed that the interaction between the Anjasmoro gene mutation and Water Saturated Stress gave a very significant effect on the growth and production of soybean plants, namely plant height (95.11 cm), number of leaves (26.33 strands), flowering age (31.67 days), dry weight. plants (212.69 grams), root dry weight (9.88 grams), number of pods (76.78 seeds), seed weight planted (23.04 grams), seed weight per plot (1.73 kg) production per hectare (2.88 tons), and harvest age (74.33 days).
Clinicobiochemical Parameters of Cytomegalovirus IgM and IgG positive Biliary Atresia and their Relation with Serological Titer in Infants.
Authors: Akter Sharmin , Halim Kazi Shafiqul , Mazumder Wahiduzzaman , Alam Rubayat , Nahid Khan Lamia , Rukunuzzaman Md 
Published: June 2024

Cytomegalovirus (CMV) associated Biliary atresia (BA) is one of the clinical classification of Biliary atresia (BA). There is a hypothesis that CMV IgM positive BA is a clinically different entity and prognosis is poor. The aim of this study was to evaluate the clinical and biochemical parameters of CMV IgM and CMV IgG positive BA in one to six month old infants and their relation with serological titer. This cross-sectional study was carried out from January 2019 to June 2022 in the department of Pediatric Gastroenterology and Nutrition, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka. A total of 48 infants were included in this study as study subject who were diagnosed as biliary atresia with positive CMV IgM & CMV IgG. After taking written informed consents data were taken from parents or legal guardians by using a structured questionnaire. Data were analyzed by statistical package for social sciences (SPSS), version- 23. Majority of the cases (58.3%) were in 2-4 month of age group, 70.8% were male and male-female ratio was almost 2.5:1. Regarding birth history most of the infants (95.8%) were term baby and two-third (66.7%) of them was appropriate for gestational age (AGA). This study observed the onset of jaundice among the infants, here 60.4% of them detected jaundice jaundice within 7 days, 22.9% within 7-14 days and 16.7% after 14 days. About three fourth (73%) of infants presented with intermittent pale stool and more than one-fourth (27%) had persistent pales tool. One fourth 12(25%) patient had features of coagulopathy. The mean of total bilirubin, direct bilirubin, alanine transaminase (ALT) and gamma-glutamyl transpeptidase (GGT ) were 11.89±4.0, 8.27±3.42, 162.67±103.09 and 669.46±543.57 respectively; The mean of the titers of CMV IgM and CMV IgG were 50.84±33.09 and 82.40±53.79 respectively. The prolong international normalised ratio (INR ) was in 30.8% of infants and mean INR was 2.44±2.54. Study finds that CMV IgM titer level was correlated with total bilirubin (r=-0.256; p<.05) and direct bilirubin (r=-0.365; p<.05); where CMV IgG titer level was correlated with age (r=-0.362; p<.05) and INR (r=0.271; p<.05). It may be concluded as increase in age would increase titer of IgG and increase in titer of IgG is associated with increase coagulopathy. Elevated levels of CMV IgM titer correlated with bilirubin level or cholestasis.
Bangladesh Med J. 2023 Jan; 52(1): 12-17
Peran Guru Dalam Pendampingan Ekplorasi Pembelajaran Sains di TK Tunas Mekar Sari Denpasar
Authors: Adnyani Dewi I Gusti Ayu Cintya Widi , Suardika I Komang 
Published: June 2024

Peranan guru dalam pendampingan sangat penting. Berbagai penelitian telah dilakukan tentang peran guru pada pembelajaran sains sederhana pada anak. Tujuan penelitian: Pertama, untuk mengetahui bagaimana peran guru dalam pendampingan proses ekplorasi anak pada pembelajaran sains. Kedua, untuk mengetahui bagaimana implikasinya terhadap anak, guru, dan lingkungan sekolah.Penelitian menggunakan metode kualitatif dengan pendekatan deskriptif. Sumber data adalah kepala sekolah, guru, dan anak-anak di TK Tunas Mekar Sari Denpasar. Teknik pengambilan data dengan wawancara, observasi dan studi kepustaakan. Analisis data dilakukan dengan teori humanisme Ki Hajar Dewantara. Hasil penelitian menunjukkan : 1. peran guru sebagai pendamping, fasislitator, dan tutor (pengajar) 2. Implikasinya bagi anak-anak; anak memperoleh penglaman langsung dengan tahu, nama-nama tanaman dan lingkungan, mengerti proses sains sederhana dengan bahan alam. Bagi guru; guru dapat secara kreatif dan mudah mengenalkan dan memanfaatkan media alam sebagai bahan sains sederhana. Bagi lingkungan; keberlangsungan hidup lingkungan akan terjaga. Kesimpulan penelitian ini adalah pada setiap perannya guru dapat selalu mendampingi anak usia dini dalam memaksimalkan proses ekplorasi termasuk bekerjasama dengan orang tua guna keberhasilan pembelajaran.
ANALISIS PERKEMBANGAN SOSIAL EMOSIONAL ANAK USIA 4-5 TAHUN DITINJAU DARI PENERAPAN APE BUSY BOOK DI TK TA PENDEM 01
Authors: Ayatina Nurul , Zulfahmi Muhammad Nofan 
Published: June 2024

Perkembangan sosial emosional merupakan proses dimana anak belajar untuk berperilaku yang berhubungan dengan individu untuk hidup dan berinteraksi atau melakukan kegiatan sosial dengan individu lain dalam kehidupan sehari hari. Perkembangan sosial emosional dapat dikembangkan dengan melalui proses pembelajaran. penelitian ini memiliki tujuan untuk mendeskripsikan dan mengetahui perkembangan sosial emosional yang terjadi pada anak usia 4-5 tahun melalui permainan busy book. Pendekeatan penelitian yang digunakan dalam penelitian ini dengan menggunakan pendekatan penelitian kualitatif dengan jenis deskriptif kualitatif untuk mendeskripsikan terkait implementasi APE busy book sebagai peninjau dari capaian perkembangan anak usia 4–5 tahun di lembaga TK TA Pendem 01. Penelitian ini menggunakan teknik pengumpulan data observasi, wawancara dan dokumentasi. Penelitian yang dilakukan menjelaskan bahwa dengan menggunakan media busy book anak sudah mencapai indikator yang diteliti oleh peneliti yaitu anak mampu berantusias dalam mengikuti rangkaian permainan yang ada di busy book, menaati peraturan yang berlaku dalam permainan, percaya diri untuk menjawab pertanyaan yang dikasih pendidik dan mampu berinteraksi dengan sesama teman.
UJI AKTIVITAS ANTIBAKTERI SEDUHAN KOMBINASI JAHE (Zingiber officinale), SEREH (Cimbopogon citratus) DAN KAYU MANIS (Cinnamomi burmanii) TERHADAP BAKTERI Eschericia colli
Authors: Khairunnisa  , Sri Idawati  , Ajeng Dian Pertiwi  , Sri Rahmawati  
Published: June 2024

Kontaminasi mikroba pada saat ini adalah salah satu masalah yang dihadapi dalam kehidupan manusia yang berkaitan dengan penyebab penyakit infeksi. Pengobatan penyakit infeksi pada umumnya menggunakan obat antibiotik. Namun, penggunaan antibiotik secara berlebihan dapat menimbulkan resiko seperti resistensi bakteri. Salah satu pilihan alternatif pengganti antibiotika dengan menggunakan obat tradisional yang berasal dari tanaman seperti Rimpang Jahe (Zingiber officinale), Batang Sereh (Cimbopogon citratus), Dan Kulit Batang Kayu Manis (Cinnamomi burmanii). Tujuan dari penelitian ini yaitu untuk mengetahui aktivitas antibakteri seduhan kombinasi jahe, sereh, dan kayu manis terhadap bakteri Eschericia colli serta untuk mengetahui berapa konsentrasi yang paling efektif sebagai antibakteri dari seduhan kombinasi jahe, sereh, dan kayu manis terhadap bakteri Eschericia colli. Metode dalam penelitian ini menggunakan metode true eksperimental dimana seduhan kombinasi jahe,sereh,dan kayu manis dibuat sebanyak empat konsentrasi yaitu 60%, 45%, 30%, 15% , dengan kontrol positif (+) antibiotik chloramphenicol 250 mg dan kontrol negatif (-) menggunakan aquades. Hasil penelitian menunjukkan bahwa tidak adanya zona hambat yang terbentuk pada masing-masing konsentrasi dan kontrol negatif (-), zona hambat yang terbentuk hanya pada kontrol positif (+) dengan diameter rata-rata 24,25 dengan kategori sangat kuat.
Puberty classifications in beef heifers are moderately to highly heritable and associated with candidate genes related to cyclicity and timing of puberty
Authors: Hess Melanie K. , Mersha Anteneh , Ference Sadie S. , Nafziger Sarah R. , Keane Jessica A. , Fuller Anna M. , Kurz Scott G. , Sutton Courtney M. , Spangler Matthew L. , Petersen Jessica L. , Cupp Andrea S. 
Published: June 2024

Introduction: Pubertal attainment is critical to reproductive longevity in heifers. Previously, four heifer pubertal classifications were identified according to attainment of blood plasma progesterone concentrations &gt; 1 ng/ml: 1) Early; 2) Typical; 3) Start-Stop; and 4) Non-Cycling. Early and Typical heifers initiated and maintained cyclicity, Start-Stop started and then stopped cyclicity and Non-Cycling never initiated cyclicity. Start-Stop heifers segregated into Start-Stop-Discontinuous (SSD) or Start-Stop-Start (SSS), with SSD having similar phenotypes to Non-Cycling and SSS to Typical heifers. We hypothesized that these pubertal classifications are heritable, and loci associated with pubertal classifications could be identified by genome wide association studies (GWAS).Methods: Heifers (n = 532; 2017 – 2022) genotyped on the Illumina Bovine SNP50 v2 or GGP Bovine 100K SNP panels were used for variant component estimation and GWAS. Heritability was estimated using a univariate Bayesian animal model.Results: When considering pubertal classifications: Early, Typical, SSS, SSD, and Non-Cycling, pubertal class was moderately heritable (0.38 ± 0.08). However, when heifers who initiated and maintained cyclicity were compared to those that did not cycle (Early+Typical vs. SSD+Non-Cycling) heritability was greater (0.59 ± 0.19). A GWAS did not identify single nucleotide polymorphisms (SNPs) significantly associated with pubertal classifications, indicating puberty is a polygenic trait. A candidate gene approach was used, which fitted SNPs within or nearby a set of 71 candidate genes previously associated with puberty, PCOS, cyclicity, regulation of hormone secretion, signal transduction, and methylation. Eight genes/regions were associated with pubertal classifications, and twenty-two genes/regions were associated with whether puberty was attained during the trial. Additionally, whole genome sequencing (WGS) data on 33 heifers were aligned to the reference genome (ARS-UCD1.2) to identify variants in FSHR, a gene critical to pubertal attainment. Fisher’s exact test determined if FSHR SNPs segregated by pubertal classification. Two FSHR SNPs that were not on the bovine SNP panel were selected for additional genotyping and analysis, and one was associated with pubertal classifications and whether they cycled during the trial.Discussion: In summary, these pubertal classifications are moderately to highly heritable and polygenic. Consequently, genomic tools to inform selection/management of replacement heifers would be useful if informed by SNPs associated with cyclicity and early pubertal attainment.
The effect of smoking in inflammatory bowel disease outcomes
Authors: Alzahrani Basil A. , Aljuhani Jamal A. , Badri Ziyad A. , Alshamrani Rayan M. , Algaows Faisal Suliman , Ahmed Mohamed Eldigire 
Published: June 2024

BackgroundThe hallmark of inflammatory bowel disease (IBD) is a persistent inflammation of the digestive system brought on by an imbalance in the gut microbiota. The two IBDs that are most well-known are ulcerative colitis (UC) and Crohn’s disease (CD). The purpose of the current study was to evaluate the Impact of smoking in inflammatory bowel disease.MethodsData of 373 patients with IBD divided into 209 Crohn’s disease and 164 with ulcerative colitis disease. cases were collected from king Abdulaziz medical city, Jeddah, Saudi Arabia from 2016-2022. Patients older than 18 years, Patients with a history of cancer other than colon cancer or other GIT disorders were excluded from consideration.ResultThis study involved 373 participants, predominantly with Crohn’s disease 209. The participants exhibited diverse smoking habits, with complications observed in 53.6% of cases. Smoking status and duration showed significant associations with complications, particularly in Crohn’s disease. For ulcerative colitis, smoking correlated with lower complication rates. The analysis of smoking variables and disease remission highlighted nuanced relationships, emphasizing the need for further exploration. Specific associations between smoking and Crohn’s disease site or ulcerative colitis subtypes were observed, suggesting potential influences on disease manifestations.ConclusionAccording to our research, smoking is negatively correlated with experiencing problems from ulcerative colitis (UC), and patients who smoke also appear to have higher rates of remission. Smoking has more issues with Crohn’s disease, though.
BMAL2 promotes eCIRP-induced macrophage endotoxin tolerance
Authors: Zhou Mian , Aziz Monowar , Li Jingsong , Jha Alok , Ma Gaifeng , Murao Atsushi , Wang Ping 
Published: June 2024

BackgroundThe disruption of the circadian clock is associated with inflammatory and immunological disorders. BMAL2, a critical circadian protein, forms a dimer with CLOCK, activating transcription. Extracellular cold-inducible RNA-binding protein (eCIRP), released during sepsis, can induce macrophage endotoxin tolerance. We hypothesized that eCIRP induces BMAL2 expression and promotes macrophage endotoxin tolerance through triggering receptor expressed on myeloid cells-1 (TREM-1).MethodsC57BL/6 wild-type (WT) male mice were subjected to sepsis by cecal ligation and puncture (CLP). Serum levels of eCIRP 20 h post-CLP were assessed by ELISA. Peritoneal macrophages (PerM) were treated with recombinant mouse (rm) CIRP (eCIRP) at various doses for 24 h. The cells were then stimulated with LPS for 5 h. The levels of TNF-α and IL-6 in the culture supernatants were assessed by ELISA. PerM were treated with eCIRP for 24 h, and the expression of PD-L1, IL-10, STAT3, TREM-1 and circadian genes such as BMAL2, CRY1, and PER2 was assessed by qPCR. Effect of TREM-1 on eCIRP-induced PerM endotoxin tolerance and PD-L1, IL-10, and STAT3 expression was determined by qPCR using PerM from TREM-1-/- mice. Circadian gene expression profiles in eCIRP-treated macrophages were determined by PCR array and confirmed by qPCR. Induction of BMAL2 activation in bone marrow-derived macrophages was performed by transfection of BMAL2 CRISPR activation plasmid. The interaction of BMAL2 in the PD-L1 promoter was determined by computational modeling and confirmed by the BIAcore assay.ResultsSerum levels of eCIRP were increased in septic mice compared to sham mice. Macrophages pre-treated with eCIRP exhibited reduced TNFα and IL-6 release upon LPS challenge, indicating macrophage endotoxin tolerance. Additionally, eCIRP increased the expression of PD-L1, IL-10, and STAT3, markers of immune tolerance. Interestingly, TREM-1 deficiency reversed eCIRP-induced macrophage endotoxin tolerance and significantly decreased PD-L1, IL-10, and STAT3 expression. PCR array screening of circadian clock genes in peritoneal macrophages treated with eCIRP revealed the elevated expression of BMAL2, CRY1, and PER2. In eCIRP-treated macrophages, TREM-1 deficiency prevented the upregulation of these circadian genes. In macrophages, inducible BMAL2 expression correlated with increased PD-L1 expression. In septic human patients, blood monocytes exhibited increased expression of BMAL2 and PD-L1 in comparison to healthy subjects. Computational modeling and BIAcore assay identified a putative binding region of BMAL2 in the PD-L1 promoter, suggesting BMAL2 positively regulates PD-L1 expression in macrophages.ConclusioneCIRP upregulates BMAL2 expression via TREM-1, leading to macrophage endotoxin tolerance in sepsis. Targeting eCIRP to maintain circadian rhythm may correct endotoxin tolerance and enhance host resistance to bacterial infection.
Evaluation of the clinical value of 10 estimating glomerular filtration rate equations and construction of a prediction model for kidney damage in adults from central China
Authors: Wang Xian , Xu Xingcheng , Wang Yongsheng , Liu Lei , Xu Ying , Liu Jun , Hu Benjin , Li Xiaowei 
Published: June 2024

ObjectivesThis study aimed to evaluate 10 estimating glomerular filtration rate (eGFR) equations in central China population and construct a diagnostic prediction model for assessing the kidney damage severity.MethodsThe concordance of 10 eGFR equations was investigated in healthy individuals from central China, and their clinical effectiveness in diagnosing kidney injury was evaluated. Subsequently, relevant clinical indicators were selected to develop a clinical prediction model for kidney damage.ResultsThe overall concordance between CKD-EPIASR-Scr and CKD-EPI2021-Scr was the highest (weightedκ = 0.964) in healthy population. The CG formula, CKD-EPIASR-Scr and CKD-EPI2021-Scr performed better than others in terms of concordance with referenced GFR (rGFR), but had poor ability to distinguish between rGFR &lt; 90 or &lt; 60 mL/min·1.73 m2. This finding was basically consistent across subgroups. Finally, two logistic regression prediction models were constructed based on rGFR &lt; 90 or 60 mL/min·1.73 m2. The area under the curve of receiver operating characteristic values of two prediction models were 0.811 vs 0.846 in training set and 0.812 vs 0.800 in testing set.ConclusionThe concordance of CKD-EPIASR-Scr and CKD-EPI2021-Scr was the highest in the central China population. The Cockcroft-Gault formula, CKD-EPIASR-Scr, and CKD-EPI2021-Scr more accurately reflected true kidney function, while performed poorly in the staging diagnosis of CKD. The diagnostic prediction models showed the good clinical application performance in identifying mild or moderate kidney injury. These findings lay a solid foundation for future research on renal function assessment and predictive equations.