bluuebunny/crossref_metadata_2025_split huggingface dataset:
Changes in global gene expression during in vitro decidualization of rat endometrial stromal cells
Authors: Vallejo Griselda , Maschi Darío , Mestre‐Citrinovitz Ana C. , Aiba Kazuhiro , Maronna Ricardo , Yohai Victor , Ko Minoru S.H. , Beato Miguel , Saragüeta Patricia 
Published: September 2009

AbstractDuring the preimplantation phase of pregnancy the endometrial stroma differentiates into decidua, a process that implies numerous morphological changes and is an example of physiological transdifferentiation. Here we show that UIII rat endometrial stromal cells cultured in the presence of calf serum acquired morphological features of decidual cells and expressed decidual markers. To identify genes involved in decidualization we compared gene expression patterns of control and decidualized UIII cells using cDNA microarray. We found 322 annotated genes exhibiting significant differences in expression (>3‐fold, fold discovery rate (FDR) >0.005), of which 312 have not been previously related to decidualization. Analysis of overrepresented functions revealed that protein synthesis, gene expression, and chromatin architecture and remodeling are the most relevant modified functions during decidualization. Relevant genes are also found in the functional terms differentiation, cell proliferation, signal transduction, and matrix/structural proteins. Several of these new genes involved in decidualization (Csdc2, Trim27, Eef1a1, Bmp1, Wt1, Aes, Gna12, and Men1) are shown to be also regulated in uterine decidua during normal pregnancy. Thus, the UIII cell culture model will allow future mechanistic studies to define the transcriptional network regulating reprogramming of stromal cells into decidual cells. J. Cell. Physiol. 222:127–137, 2010. © 2009 Wiley‐Liss, Inc.
Expression of estrogen receptor β increases integrin α1 and integrin β1 levels and enhances adhesion of breast cancer cells
Authors: Lindberg Karolina , Ström Anders , Lock John G. , Gustafsson Jan‐Åke , Haldosén Lars‐Arne , Helguero Luisa A. 
Published: September 2009

AbstractEstrogen effects on mammary gland development and differentiation are mediated by two receptors (ERα and ERβ). Estrogen‐bound ERα induces proliferation of mammary epithelial and cancer cells, while ERβ is important for maintenance of the differentiated epithelium and inhibits proliferation in different cell systems. In addition, the normal breast contains higher ERβ levels compared to the early stage breast cancers, suggesting that loss of ERβ could be important in cancer development. Analysis of ERβ−/− mice has consistently revealed reduced expression of cell adhesion proteins. As such, ERβ is a candidate modulator of epithelial homeostasis and metastasis. Consequently, the aim of this study was to analyze estrogenic effects on adhesion of breast cancer cells expressing ERα and ERβ. As ERβ is widely found in breast cancer but not in cell lines, we used ERα positive T47‐D and MCF‐7 human breast cancer cells to generate cells with inducible ERβ expression. Furthermore, the colon cancer cell lines SW480 and HT‐29 were also used. Integrin α1 mRNA and protein levels increased following ERβ expression. Integrin β1—the unique partner for integrin α1—increased only at the protein level. ERβ expression enhanced the formation of vinculin containing focal complexes and actin filaments, indicating a more adhesive potential. This was confirmed by adhesion assays where ERβ increased adhesion to different extracellular matrix proteins, mostly laminin. In addition, ERβ expression was associated to less cell migration. These results indicate that ERβ affects integrin expression and clustering and consequently modulates adhesion and migration of breast cancer cells. J. Cell. Physiol. 222:156–167, 2010. © 2009 Wiley‐Liss, Inc.
Bradykinin protects against brain microvascular endothelial cell death induced by pathophysiological stimuli
Authors: Bovenzi Veronica , Savard Martin , Morin Josée , Cuerrier Charles M. , Grandbois Michel , Gobeil Fernand 
Published: September 2009

AbstractThe morphological and functional integrity of the microcirculation is compromised in many cardiovascular diseases such as hypertension, diabetes, stroke, and sepsis. Angiotensin converting enzyme inhibitors (ACEi), which are known to favor bradykinin (BK) bioactivity by reducing its metabolism, may have a positive impact on preventing the microvascular structural rarefaction that occurs in these diseases. Our study was designed to test the hypothesis that BK, via B2 receptors (B2R), protects the viability of the microvascular endothelium exposed to the necrotic and apoptotic cell death inducers H2O2 and LPS independently of hemodynamics. Expression (RT‐PCR and radioligand binding) and functional (calcium mobilization with fura‐2AM, and p42/p44MAPK and Akt phosphorylation assays) experiments revealed the presence of functional B2R in pig cerebral microvascular endothelial cells (pCMVEC). In vitro results showed that the cytocidal effects of H2O2 and LPS on pCMVEC were significantly decreased by a BK pretreatment (MTT and crystal violet tests, annexin‐V staining/FACS analysis), which was countered by the B2R antagonist HOE 140. BK treatment coincided with enhanced expression of the cytoprotective proteins COX‐2, Bcl‐2, and Cu/ZnSOD. Ex vivo assays on rat brain explants showed that BK impeded (by ∼40%) H2O2‐induced microvascular degeneration (lectin‐FITC staining). The present study proposes a novel role for BK in microvascular endothelial protection, which may be pertinent to the complex mechanism of action of ACEi explaining their long‐term beneficial effects in maintaining vascular integrity. J. Cell. Physiol. 222:168–176, 2010. © 2009 Wiley‐Liss, Inc.
Saturated fatty acid‐induced insulin resistance is associated with mitochondrial dysfunction in skeletal muscle cells
Authors: Hirabara Sandro M. , Curi Rui , Maechler Pierre 
Published: September 2009

AbstractIncreased plasma levels of free fatty acids (FFA) occur in states of insulin resistance such as obesity and type 2 diabetes mellitus. These high levels of plasma FFA are proposed to play an important role for the development of insulin resistance but the mechanisms involved are still unclear. This study investigated the effects of saturated and unsaturated FFA on insulin sensitivity in parallel with mitochondrial function. C2C12 myotubes were treated for 24 h with 0.1 mM of saturated (palmitic and stearic) and unsaturated (oleic, linoleic, eicosapentaenoic, and docosahexaenoic) FFA. After this period, basal and insulin‐stimulated glucose metabolism and mitochondrial function were evaluated. Saturated palmitic and stearic acids decreased insulin‐induced glycogen synthesis, glucose oxidation, and lactate production. Basal glucose oxidation was also reduced. Palmitic and stearic acids impaired mitochondrial function as demonstrated by decrease of both mitochondrial hyperpolarization and ATP generation. These FFA also decreased Akt activation by insulin. As opposed to saturated FFA, unsaturated FFA did not impair glucose metabolism and mitochondrial function. Primary cultures of rat skeletal muscle cells exhibited similar responses to saturated FFA as compared to C2C12 cells. These results show that in muscle cells saturated FFA‐induced mitochondrial dysfunction associated with impaired insulin‐induced glucose metabolism. J. Cell. Physiol. 222:187–194, 2010. © 2009 Wiley‐Liss, Inc.
Mutations in the external loops of BK virus VP1 and urine viral load in renal transplant recipients
Authors: Tremolada Sara , Delbue Serena , Castagnoli Lorenzo , Allegrini Sara , Miglio Umberto , Boldorini Renzo , Elia Francesca , Gordon Jennifer , Ferrante Pasquale 
Published: September 2009

AbstractPolyomavirus‐associated nephropathy (PVAN) is a major complication that occurs after renal transplantation and is induced by reactivation of the human polyomavirus BK (BKV). The structure of the viral capsid protein 1 (VP1) is characterized by the presence of external loops, BC, DE, EF, GH, and HI, which are involved in receptor binding. The pathogenesis of PVAN is not well understood, but viral risk factors are thought to play a crucial role in the onset of this pathology. In an attempt to better understand PVAN pathogenesis, the BKV‐VP1 coding region was amplified, cloned, and sequenced from the urine of kidney transplant recipients who did, and did not, develop the pathology. Urine viral loads were determined by using real time quantitative PCR (Q‐PCR). Amino acid substitutions were detected in 6/8 patients, and 6/7 controls. The BC and EF loop regions were most frequently affected by mutations, while no mutations were found within the GH and HI loops of both patients and controls. Some mutations, that were exclusively detected in the urine of PVAN patients, overlapped with previously reported mutations, although a correlation between changes in amino acids and the development of PVAN was not found. Urine viral loads were higher than that of the proposed cut‐off loads for identification of patients that are at a high risk of developing PVAN (107 copies/ml), both in the PVAN and control groups, thus confirming that urine viral load is not a useful predictive marker for the development of PVAN. J. Cell. Physiol. 222:195–199, 2010. © 2009 Wiley‐Liss, Inc.
Selective control of gene expression by CDK9 in human cells
Authors: Garriga Judit , Xie Hongbo , Obradovic Zoran , Graña Xavier 
Published: September 2009

AbstractCDK9 associates with T‐type cyclins and positively regulates transcriptional elongation by phosphorylating RNA polymerase II (RNAPII) and negative elongation factors. However, it is unclear whether CDK9 is required for transcription of most genes by RNAPII or alternatively plays a role regulating the expression of restricted subsets of genes. We have investigated the direct effects of inhibiting cellular CDK9 activity in global gene expression in human cells by using a dominant‐negative form of CDK9 (dnCDK9). We have also compared direct inhibition of cellular CDK9 activity to pharmacological inhibition with flavopiridol (FVP), a CDK inhibitor that potently inhibits CDK9 and cellular transcription. Because of its presumed selectivity for CDK9, FVP has been previously used as a tool to infer the role of CDK9 on global gene expression. DNA microarray analyses described here show that inhibition of gene expression by FVP is consistent with global inhibition of transcription. However, specific inhibition of CDK9 activity with dnCDK9 leads to a distinctive pattern of changes in gene expression, with more genes being specifically upregulated (122) than downregulated (84). Indeed, the expression of many short‐lived transcripts downregulated by FVP is not modulated by dnCDK9. Nevertheless, consistently with FVP inhibiting CDK9 activity, a significant number of the genes downregulated/upregulated by dnCDK9 are modulated with a similar trend by FVP. Our data suggests that the potent effects of FVP on transcription are likely to involve inhibition of CTD kinases in addition to CDK9. Our data also suggest complex and gene‐specific modulation of gene expression by CDK9. J. Cell. Physiol. 222:200–208, 2010. © 2009 Wiley‐Liss, Inc.
Protective effects of vitamins C and E on spermatogenesis of 1.5 Tesla magnetic field exposed rats
Authors: Monfared Ali Shabestani , Jorsaraei Seyed Gholam Ali , Abdi Rooholah 
Published: September 2009

AbstractPurposeTo investigate protective effects of vitamins E and C against 1.5 Tesla static magnetic fields in magnetic resonance imaging (MRI) on spermatogenesis parameters was the main goal of the present study.Materials and MethodsNinety‐two mature male rats were exposed to 1.5 T MRI static magnetic fields for 30 min with or without vitamins C and E alone or in combination. Animals were sacrificed and the testicular tissues were anatomically sectioned, stained, and the number of germ cells and the diameters of sperm ducts were measured and compared with sham and controls.ResultsResults showed that compared to sham, static magnetic fields may reduce the germ cell count (P = 0.000) and sperm ducts diameters (P = 0.020), and vitamins C and E could modify the reduction in germ cell count (P = 0.019) but they did not show any protective effect on sperm duct diameter reduction (0.647).ConclusionThe protective effects of vitamins C and E are different, and depend on the type of effects. It seems that the modifying effects of vitamins are to be additive, but vitamin E plays a more important role than vitamin C against the static magnetic field on spermatogenesis parameters in clinical MRI. J. Magn. Reson. Imaging 2009. © 2009 Wiley‐Liss, Inc.
Proximal femur bone marrow blood perfusion indices are reduced in hypertensive rats: A dynamic contrast‐enhanced MRI study
Authors: Zhang Ya‐Feng , Wang Yi‐Xiang J. , Griffith James F. , Kwong William K.M. , Ma Heather T. , Qin Ling , Kwok Timothy C.Y. 
Published: September 2009

AbstractPurpose:To investigate the differences in proximal femoral bone marrow blood perfusion indices between hypertensive and normotensive rats using perfusion magnetic resonance imaging (MRI).Materials and Methods:Six‐month‐old male spontaneously hypertensive rats (SHR) and Wistar Kyoto rats (WKY) were used (12 of each). Dynamic contrast‐enhanced MRI of the right hip was performed after bolus injection of Gd‐DOTA administered through a tail vein cannula. In all, 800 images were acquired at 0.6 sec/image. Regions of interest (ROIs) were drawn comprising the medullary component of proximal femoral shaft and femoral head. MRI maximum enhancement (Emax) and enhancement slope (Eslope) were analyzed.Results:The Emax and Eslope of proximal femoral shaft and femoral head of SHR were significantly lower than those of WKY (Emax: 107.4 ± 18.2% vs. 130.6 ± 21.5%, P = 0.009, and 76.0 ± 12.5% vs. 97.9 ± 6.9%, P < 0.001, respectively; Eslope: 3.01 ± 0.63%/sec vs. 3.75 ± 0.74%/sec, P = 0.016, and 1.95 ± 0.33%/sec vs. 2.28 ± 0.28%/sec, P = 0.012, respectively). The Emax and Eslope of femoral head were significantly lower than those of proximal femoral shaft in both SHR and WKY (P < 0.001). In both SHR and WKY, proximal femoral shaft and femoral head had a rather different contrast enhancement pattern.Conclusion:Proximal femoral shaft and femoral head bone marrow blood perfusion indices were significantly lower in hypertensive rats than in normotensive rats. Femoral head bone marrow was less perfused than proximal femoral shaft in both rats. J. Magn. Reson. Imaging 2009. © 2009 Wiley‐Liss, Inc.
DSM‐VPTSD diagnostic criteria for children and adolescents: A developmental perspective and recommendations
Authors: Pynoos Robert S. , Steinberg Alan M. , Layne Christopher M. , Briggs Ernestine C. , Ostrowski Sarah A. , Fairbank John A. 
Published: September 2009

AbstractTheDiagnostic and Statistical Manual of Mental Disorders, Fifth Edition (DSM‐V)should ensure systematic attention to age‐specific manifestations and selective modifications of the diagnostic criteria for posttraumatic stress disorder (PTSD) among children and adolescents. The authors propose developmental refinements to the conceptual framework for PTSD based on an appreciation of the different neurosignatures of danger and safety, and maturational processes that underlie symptom presentation. This includes preliminary evidence for the developmental salience of additional dimensions for PTSD (e.g., recklessness and thrill‐seeking). The authors provide conservative recommendations forDSM‐Vdiagnostic criteria that primarily highlight age‐related developmental manifestations that, if included in the accompanying text, would bring a richer appreciation of developmentally linked symptom presentations.
Reformation of concha bullosa following treatment by crushing surgical technique: Implication for balloon sinuplasty
Authors: Kieff David A. , Busaba Nicolas Y. 
Published: September 2009

AbstractObjectives/Hypothesis:There are various surgical techniques designed to treat conchae bullosae (CB). These include partial or total resection and crushing. Frontal sinus balloon sinuplasty is thought to work by the crushing/remodeling of the agger nasi and frontal recess air cells. The long‐term outcome of sinuplasty in the frontal/ethmoid air cell region is unknown. To date, no study has been done on the reformation of CB after crushing. We report on the long‐term outcome of a series of patients who underwent crushing of their CB and suggest implications for frontal sinus balloon sinuplasty.Study Design:Retrospective case series.Methods:Retrospective review of 10 patients who re‐presented with CB as a component of their nasal obstructive symptoms despite previously undergoing crushing of their CB. Data analyzed included paranasal sinus computed tomography (CT) scans and operative reports.Results:These 10 patients re‐presented with recurrent rhinosinusitis and nasal obstructive symptoms. The patients had previously undergone septoplasty surgery with crushing of the CB and were noted to have reformed the CB on their most recent sinus CT. The previous surgeries were preformed from 2 to 15 years prior to their representation.Conclusions:CB can reform following crushing technique. One may extrapolate that agger nasi and frontal recess air cells may reform following balloon sinuplasty leading to recurrent obstruction of the frontal sinus outflow tract. Laryngoscope, 2009
A prospective study of paranasal sinus osteomas in 1,889 cases: Changing patterns of localization
Authors: Erdogan Nezahat , Demir Ugur , Songu Murat , Ozenler Nesibe Kurt , Uluç Engin , Dirim Berna 
Published: September 2009

AbstractObjectives/Hypothesis:We developed the largest paranasal sinus computed tomography (CT) scan study so far by including 1,889 cases to investigate the prevalence, localization, age distribution, and the secondary complications of paranasal sinus osteomas.Study Design:Prospective study.Methods:A prospective study was performed on 1,889 consecutive adult individuals who underwent paranasal sinus CT examinations with suspected sinus disease.Results:The prevalence of paranasal sinus osteomas was determined to be 3%. Osteomas were located most frequently in the ethmoid sinuses. The size of the osteomas varied from approximately from 2 to 30 mm. Of the osteomas found, 37%were accompanied by pathological sinonasal findings.Conclusions:The prevalence of sinonasal osteoma in the paranasal sinus region detected by CT scan is supporting the literature, whereas the localization pattern challenges it. Laryngoscope, 2009
Radiotherapy for basal cell carcinoma of the medial canthus region
Authors: Swanson Erika L. , Amdur Robert J. , Mendenhall William M. , Morris Christopher G. , Kirwan Jessica M. , Flowers Franklin 
Published: September 2009

AbstractObjectives/Hypothesis:To report outcome for patients treated with radiotherapy (RT) for basal cell carcinoma of the medial canthus.Study Design:Retrospective review.Methods:Thirty‐three patients treated with RT at the University of Florida between 1965 and 2005 for basal cell carcinoma of the medial canthus were retrospectively reviewed. RT was the primary treatment for gross disease in 70% of patients and for positive margin after resection in 30%. The prescribed dose was 50 to 60 Gy at 2.0 to 2.5 Gy per fraction.Results:Surviving patients were followed for a median of 14 years. Tumor recurred at the primary site in 10%. There were no regional recurrences or distant metastases. The local control rate was 100% in patients treated with surgery followed by RT for positive margins. In patients treated with RT alone, the local control rate was 94% with de novo lesions and 67% if the lesion was recurrent after prior surgery. Cause‐specific survival was 95% at 10 years; overall survival was 52% at 10 years. There were no severe complications. Chronic epiphora was present in 21% and chronic dry eye symptoms in 3%.Conclusions:With the proper technique, RT produces excellent results in several of these patients. Patients with recurrent tumors and gross disease at the time of RT have a suboptimal cure rate. Our plan is to increase the RT dose to 64.8 Gy at 1.8 Gy per fraction. Laryngoscope, 2009
Endoscopy‐assisted surgery with topical mitomycin for a cholesterol granuloma in the petrous apex
Authors: Haginomori Shin‐Ichi , Mori Atsuko , Kanazawa Atsuko , Takenaka Hiroshi 
Published: September 2009

AbstractObjectives/Hypothesis:We present the case of a 21‐year‐old woman who underwent endoscopy‐assisted surgery with topical mitomycin for a large cholesterol granuloma in the petrous apex that involved the eustachian tube.Study Design:Case report.Methods:Minimally invasive surgery was performed on the cholesterol granuloma with endoscopic assistance, and topical mitomycin (0.4 mg/mL) was administered to prevent scarring of the newly created drainage/ventilation route. With the endoscopic approach, the drainage/ventilation route was secured not only from the tympanic cavity, but also using a guidewire inserted from the pharyngeal orifice of the eustachian tube.Results:The drainage/ventilation route between the nasopharynx and middle ear cavity involving the cholesterol granuloma has been preserved more than for 9 months postoperatively. Her hearing has recovered.Conclusions:This less invasive technique is a surgical option for patients with a large cholesterol granuloma in the petrous apex involving the eustachian tube. Laryngoscope, 2009
Electroactive Polymer with Oligoanilines in the Main Chain: Synthesis, Characterization and Dielectric Properties
Authors: Zhang Junfeng , Chao Danming , Cui Lili , Liu Xincai , Zhang Wanjin 
Published: September 2009

AbstractAn electroactive polymer with well‐defined oligoanilines in the main chain has been prepared by oxidative coupling polymerization. The detailed characteristics of the polymer were systematically studied by FT‐IR, 1H NMR, GPC and XRD. Its electrochemical behavior was explored with UV‐Vis spectra and cyclic voltammetry showing that its intrinsic electroactivity was maintained and exhibited three distinct reversible oxidative states. Its electrical conductivity is about 3.91 × 10−4 S · cm−1 in HCl‐doped form at room temperature. Furthermore, the polymer displays a dielectric constant of 48.4 and 13.6 at 10 and 1 MHz, respectively, mainly attributed to the improvement in delocalized charge density and electrical conductivity of the polymer by introduction of the conjugated oligoaniline segments.magnified image
Sequence Analysis of an Isocyanate Oligomer by MALDI‐TOF Mass Spectrometry Using Collision Induced Dissociation
Authors: Mass Valentina , Schrepp Wolfgang , von Vacano Bernhard , Pasch Harald 
Published: September 2009

AbstractA TDI–MDI oligomer obtained by hydrolysis of a crosslinked polyurethane (PU) was analysed regarding the oligomer distribution by standard MALDI‐TOF‐MS and the monomer sequence in the different oligomers by collision induced dissociation (CID) experiments. The CID experiments produced product ions that were selective towards the monomer sequences in different oligomer molecules. By selecting suitable oligomer precursor ions with different monomer sequences out of the total oligomer distribution and conducting CID experiments, specific product mass peaks were identified that were characteristic for random and blocky monomer sequences. Therefore, a mass spectrometric method has become available for the important class of PUs that is not only selective towards the number and type of monomer units but also towards their sequence in the oligomer chain.magnified image