bluuebunny/crossref_metadata_2025_split huggingface dataset:
Astrocyte elevated gene‐1 interacts with β‐catenin and increases migration and invasion of colorectal carcinoma
Authors: Zhang Fenfen , Yang Qingxu , Meng Fengjiao , Shi Huijuan , Li Hui , Liang Yingjie , Han Anjia 
Published: March 2012

AbstractTo investigate the astrocyte elevated gene‐1 (AEG‐1) expression and its relationship with the clinicopathological features of colorectal carcinoma (CRC) and β‐catenin signaling pathway. Real‐time PCR, Western blot, immunohistochemistry, and immunofluorescence staining were performed to detect AEG‐1 expression in CRC cell lines, 8 pairs of fresh CRC and adjacent nontumor tissues (ANT), 120 pairs of paraffin‐embedded CRC specimens and ANT tissues, and 60 samples of lymph node metastatic CRC tissues. Scratch wound assay and transwell matrix penetration assay were performed to determine migration and invasion of SW480 cell lines with stable AEG‐1 overexpression or SW620 cell lines with AEG‐1 knockdown. AEG‐1 expression was upregulated in CRC cell lines and tissues compared with ANT. Furthermore, AEG‐1 expression level significantly correlated with UICC stage, and the N classification. AEG‐1 overexpression significantly enhanced migration and invasion of SW480 cell lines. However, AEG‐1 knockdown suppressed migration and invasion of SW620 cell lines. Meanwhile, there was a positive correlation between AEG‐1 high expression and β‐catenin nuclear expression in CRC. AEG‐1 overexpression increased nuclear β‐catenin accumulation in CRC cell lines. AEG‐1 knockdown decreased nuclear β‐catenin accumulation in CRC cell lines. Moreover, we firstly found that AEG‐1 interacted with β‐catenin in SW480 cell lines. Our results for the first time showed that AEG‐1 interacted with β‐catenin in CRC cells and AEG‐1 expression was closely associated with progression of CRC. AEG‐1 might be a potential therapeutic target in CRC. © 2012 Wiley Periodicals, Inc.
Thumb reconstruction with a wrap‐around free flap in hand radiation injury
Authors: Lee Jung Il , Park Jung Ho , Park Jong Hoon , Kim Yong Jin , Park Jong Woong 
Published: March 2012

AbstractWe describe a patient with hand radiation injury that was caused by 192Ir radiation source exposure. The cutaneous symptoms that appear after local radiation exposure follow a certain time pattern consisting of the prodromal, manifestation, subacute, chronic, and late stages. Although the clinical characteristics of each stage are well known, limited cases of photographic demonstrations to the progressive local radiation reaction have been reported. We demonstrate characteristics of serial necrotic changes in the fingers after radiation exposure in photographs. Initially, blisters, mild erythema, and swelling were present in the exposed fingers. However, at 3 years postexposure, total necrosis, severe flexion deformity, and bony exposure were present in the exposed fingers. For restoration of hand function, we performed a transmetacarpal, metacarpophalangeal, and transphalangeal amputation of the second, third, and fourth fingers, respectively. After debridement of the necrotic thumb tissue, a wrap‐around free flap from the hallux was performed for thumb reconstruction. At 2 years postoperatively, the free flap survived well and graft bone union had occurred. The patient's hand function had improved such that he could grip a large object using the reconstructed thumb and the fifth finger. © 2012 Wiley Periodicals, Inc. Microsurgery, 2012.
An 11‐channel radio frequency phased array coil for magnetic resonance guided high‐intensity focused ultrasound of the breast
Authors: Minalga E. , Payne A. , Merrill R. , Todd N. , Vijayakumar S. , Kholmovski E. , Parker D. L. , Hadley J. R. 
Published: March 2012

AbstractIn this study, a radio frequency phased array coil was built to image the breast in conjunction with a magnetic resonance guided high‐intensity focused ultrasound (MRgHIFU) device designed specifically to treat the breast in a treatment cylinder with reduced water volume. The MRgHIFU breast coil was comprised of a 10‐channel phased array coil placed around an MRgHIFU treatment cylinder where nearest‐neighbor decoupling was achieved with capacitive decoupling in a shared leg. In addition a single loop coil was placed at the chest wall making a total of 11 channels. The radio frequency coil array design presented in this work was chosen based on ease of implementation, increased visualization into the treatment cylinder, image reconstruction speed, temporal resolution, and resulting signal‐to‐noise ratio profiles. This work presents a dedicated 11‐channel coil for imaging of the breast tissue in the MRgHIFU setup without obstruction of the ultrasound beam and, specifically, compares its performance in signal‐to‐noise, overall imaging time, and temperature measurement accuracy to that of the standard single chest‐loop coil typically used in breast MRgHIFU. Magn Reson Med, 2013. © 2012 Wiley Periodicals, Inc.
IgG anti‐Galnac‐GD1a antibodies bind to neuromuscular junctions of rat hemidiaphragm
Authors: Nagaoka Takumi , Hotta Sayako , Chiba Terumasa , Utsunomiya Iku , Abe Kenji , Yoshino Hiide , Koshikawa Chiaki , Taguchi Kyoji 
Published: March 2012

AbstractIntroduction: We investigated the localization of a ganglioside, N‐acetylgalactosaminyl GD1a (GalNAc‐GD1a), in peripheral nerves with an IgG anti–GalNAc‐GD1a antibody, which was produced in rabbits immunized with GalNAc‐GD1a. Methods: Teased fibers from ventral and dorsal roots and hemidiaphragm sections of rats were assessed using fluorescent double‐ and triple‐labeling methods. Results: The nodal and paranodal regions of teased fibers from ventral roots were immunostained with IgG anti–GalNAc‐GD1a antibodies. After collagenase treatment, no staining was seen with IgG anti–GalNAc‐GD1a or anti‐NF200 antibodies, whereas α‐bungarotoxin selectively stained nerve terminals. In cross‐sectional and longitudinal sections of rat hemidiaphragm, IgG anti–GalNAc‐GD1a antibodies overlapped with α‐BuTx and anti‐NF200 antibodies, indicating that GalNAc‐GD1a is localized to the nerve terminal. IgG anti–GalNAc‐GD1a antibody staining also overlapped with that of AChR clusters and syntaxin‐positive presynaptic nerve terminals. Conclusion: GalNAc‐GD1 is localized in both pre‐ and postsynaptic nerve terminals of neuromuscular junctions. Muscle Nerve 46: 705–710, 2012
Comment on the Analysis of Two Skeletons from Terqa, Syria
Authors: Sołtysiak Arkadiusz 
Published: March 2012

ABSTRACTRecently, a report on two human skeletons from an Early Bronze Age tomb excavated at Tell Ashara, Syria has been published in International Journal of Osteoarchaeology. One individual was identified as a warrior following these criteria: (i) size and robustness of bones, (ii) cut marks on the humerus, (iii) reduction of the ulnar styloid process, both interpreted as healed weapon‐related trauma, (iv) well‐developed muscle insertions, and (v) degenerative joint disease. Actually, none of these five criteria support the conclusion because of the following reasons: (i) not necessarily all tall and robust men become warriors, (ii) the post mortem origin of cut marks on the humerus is more likely than sharp force trauma, (iii) there are several possible causes of the unusual ulnar styloid shape other than weapon‐related trauma, (iv) the interpretation of musculoskeletal stress markers and (v) degenerative joint disease lacked control for age, sex and body size. Copyright © 2012 John Wiley & Sons, Ltd.
Novel organic–inorganic hybrid coatings prepared by the sol–gel process: corrosion and mechanical properties
Authors: Jena Kishore K , Rout Tapan K , Narayan Ramanuj , Raju Kothapalli V S N 
Published: March 2012

Abstract(Hyperbranched polyurethane‐urea)/[(3‐aminopropyl)triethoxysilane]‐ZnO (HBPUU‐APTES‐ZnO) hybrid coatings were synthesized using an inexpensive mixing technique by varying the APTES‐modified ZnO concentration. The mechanical and surface properties of the hybrid coating films were studied and compared with unmodified and modified ZnO. The corrosion, solvent and abrasion resistance show significant enhancement in HBPUU‐APTES‐ZnO hybrids and their properties are increased with increasing APTES‐ZnO concentration. This hybrid coating has opened up an opportunity for automotive topcoat application. Copyright © 2012 Society of Chemical Industry
Morphology, thermal properties, hydrophobicity and O2/N2 gas separation performance of 4,4′‐oxydiphthalic anhydride‐based polyimide/titania hybrids
Authors: Lu Hsu‐Tung , Tsai Mei‐Hui , Tseng I‐Hsiang , Chen Chiou‐Juy , Huang Shih‐Liang 
Published: March 2012

AbstractTwo 4,4′‐oxydiphthalic anhydride (ODPA)‐based polyimide (PI)/titania hybrid films with different morphologies were prepared through an in situ sol‐gel process. The precursor, poly(amic acid) (PAA), was synthesized using ODPA, diamine of 2,2‐bis[4‐(4‐aminophenoxy)phenyl]propane (BAPP) or 4,4′‐diaminodiphenyl ether (ODA) and a suitable amount of dimethylformamide solvent. A mixture of tetraethylorthotitanate (Ti(OEt)4) and acetylacetone with molar ratio of 1:4 was then added to the PAA solution and mixed thoroughly. Following curing, PI/titania hybrid membranes with different crosslinkages and Ti(OEt)4 contents were prepared. PI hybrids with the longer BAPP diamine present different morphologies and property changes related to the Ti(OEt)4 content from those of hybrids with the shorter ODA diamine. The morphologies of the two ODPA‐based PI/titania hybrids were studied with reference to the disruption of imide ring formation. Different crosslinked structures produced were identified using Fourier transform infrared analysis from the frequency shift of the CO band and relative absorbance intensities of bands of CO group and imide ring (N). Thermal properties, O2/N2 gas separation performance, contact angle, storage modulus, glass transition temperature and decomposition temperature of the PI hybrids were all found to be functions of the Ti(OEt)4 content, crosslinked structure and PI type. Copyright © 2012 Society of Chemical Industry
Anti‐androgenic effects of S‐40542, a novel non‐steroidal selective androgen receptor modulator (SARM) for the treatment of benign prostatic hyperplasia
Authors: Nejishima Hiroaki , Yamamoto Noriko , Suzuki Mika , Furuya Kazuyuki , Nagata Naoya , Yamada Shizuo 
Published: March 2012

AbstractBACKGROUNDSelective androgen receptor modulators (SARMs) would provide alternative therapeutic agent for androgen‐related diseases. We identified a tetrahydroquinoline (THQ) derivative, 1‐(8‐nitro‐3a, 4, 5, 9b‐tetrahydro‐3H‐cyclopenta[c]quinolin‐4‐yl) ethane‐1, 2‐diol (S‐40542) as a novel SARM antagonist.METHODSAffinity for nuclear receptors of S‐40542 was evaluated in receptor‐binding studies. Androgen receptor (AR) transcriptional activity of S‐40542 was investigated by luciferase reporter assay in DU145AR cells. Normal and benign prostatic hyperplasia (BPH) model rats were repeatedly treated with S‐40542 and flutamide. The tissue weights of prostate and levator ani muscle as well as blood levels of testosterone and luteinizing hormone were measured.RESULTSS‐40542 bound to the AR with high affinity. S‐40542 at relatively high concentrations increased the transcriptional activity. This agent also showed a concentration‐dependent AR antagonistic action in the presence of 1 nM 5α‐dihydrotestosterone. Repeated treatment with S‐40542 and flutamide decreased dose‐dependently the weights of the prostate to a similar extent. In contrast, the tissue weight‐reducing effect by S‐40542 treatment on the levator ani muscle was much weaker than that of flutamide. S‐40542 had little effect on the blood level of testosterone and luteinizing hormone, whereas flutamide increased the level of both hormones. Furthermore, S‐40542 decreased dose‐dependently prostate weight of BPH rats.CONCLUSIONSThe current results indicate that S‐40542 possesses the prostate‐selective SARM activity, suggestive of clinical benefit against benign prostate hyperplasia. THQ compounds may be useful for the research of mode of action of SARMs and for the development of safe SARM antagonists. Prostate 72:1580–1587, 2012. © 2012 Wiley Periodicals, Inc.
Serum sarcosine increases the accuracy of prostate cancer detection in patients with total serum PSA less than 4.0 ng/ml
Authors: Lucarelli Giuseppe , Fanelli Margherita , Larocca Angela Maria Vittoria , Germinario Cinzia Annatea , Rutigliano Monica , Vavallo Antonio , Selvaggi Francesco Paolo , Bettocchi Carlo , Battaglia Michele , Ditonno Pasquale 
Published: March 2012

AbstractBACKGROUNDSarcosine is reported to be a differential metabolite that is greatly increased during prostate cancer (PCa) progression. In this study, we assessed the role of serum sarcosine as a biomarker for PCa, as well as any association between sarcosine levels and clinical–pathological parameters.METHODSSarcosine was measured by fluorometric assay in serum samples from 290 PCa patients and 312 patients with no evidence of malignancy (NEM), confirmed by 8–12 core prostate biopsies. Nonparametric statistical tests and receiver operating characteristics (ROC) analyses were performed to assess the diagnostic performance of sarcosine in different (prostate‐specific antigen) PSA ranges.RESULTSROC analyses in subjects with PSA < 4 ng/ml showed a higher predictive value of sarcosine (AUC = 0.668) versus total PSA (AUC = 0.535) (P = 0.03), whereas for the other two PSA ranges (4–10 ng/ml and >10 ng/ml), percent ratio of free to total PSA (%fPSA) showed a predictive superiority over sarcosine. Moreover, in patients with a PSA < 4 ng/ml, the percentage of low/intermediate‐grade cancers was positively associated with sarcosine levels (P = 0.005). The specificities for serum sarcosine, %fPSA, PSA, and the logistic regression model at 95% sensitivity were 24.4, 3.41, 2.22, and 28.4%, respectively.CONCLUSIONSWe provide evidence that serum sarcosine has a higher predictive value than tPSA and %fPSA in patients with PSA < 4 ng/ml. Moreover, sarcosine levels were significantly different in low grade versus high grade cancers in this subset of patients, suggesting that this marker may be a further tool not only for diagnosing PCa in normal PSA and abnormal DRE/TRUS patients but also for selecting candidates for non‐aggressive therapies and active surveillance. Prostate 72:1611–1621, 2012. © 2012 Wiley Periodicals, Inc.
First‐principles studies on the impact of proton disorder on physical properties of ice
Authors: Wang Shaoqing 
Published: March 2012

AbstractThe random occupancy of hydrogen atoms or proton disorder is the most unique feature of ice crystal. Taking the cubic Ic ice as an example, we study the impact of proton disorder on various physical properties by first‐principles calculation in density‐functional theory for the first time. Our results show that the dielectric polarization enhances the cohesion of H2O molecules, thus increases the bulk modulus of Ic ice. The shear elastic constants are more sensitive to dielectric polarization than the elastic constant of normal strain. Ic ice is a semiconductor with a wide direct bandgap of 5.54 eV. The disorder of electric dipole moments raises the valence bands and decreases the energy bandgap. The average dielectric constant is less affected by the disorder. The present conclusions also keep valid for the more common hexagonal Ih ice based on its similar local structure with Ic ice. © 2012 Wiley Periodicals, Inc.
Fabrication of Lipid Tubules with Embedded Quantum Dots by Membrane Tubulation Protein
Authors: Tanaka Masayoshi , Critchley Kevin , Matsunaga Tadashi , Evans Stephen D. , Staniland Sarah S. 
Published: March 2012

AbstractThe first one‐dimensional (1D) assembly of low‐toxicity CuInS2/ZnS quantum dots (QDs) embedded in lipid nanotubules, formed from liposomes using the Amphiphysin‐BAR (Bin‐Amphiphysin‐Rvs domain of human amphiphysin) protein to elongate the structure, is reported. The QD‐containing lipid nanotubules display a high aspect ratio of ≈500:1 (≈40 nm diameter and 20 μm length) and are stable for more than 20 h. Furthermore, this methodology is extended to the assembly of various nanoparticle species within 1D lipid nanotubules, and includes materials such as CdSe and Au. Encapsulation within the hydrophobic core of the bilayer makes these materials highly biocompatible. The developed methodology and materials with these unique characteristics could be useful for various applications in nanobiotechnology and nanomedicine.
State‐of‐the‐Knowledge on Coating Systems for Hot Stamped Parts
Authors: Fan Dong Wei , De Cooman Bruno C. 
Published: March 2012

AbstractIn the present contribution, recent developments of coatings for hot stamped steels are reviewed. The use of bare steel in the initial hot stamping technology is discussed, including the application of lubricant oils which are used as oxidation inhibitors on bare steel surfaces. The aluminized coatings are introduced, focusing on the microstructure evolution of aluminized coatings during the hot stamping process. An analysis of the cracking of the coating, caused by the formation of brittle Fe–Al intermetallic phases and their high temperature deformation, is presented. The development of a ductile aluminide coating formed during the diffusion treatment of an aluminized coating is discussed. This aluminide coating can endure both high temperature oxidation and severe plastic deformation. The recently developed galvanized and galvannealed coatings are also reviewed and the influence of the gas atmosphere during the heating cycle on the coating stability is emphasized. The solutions which have been proposed to avoid liquid Zn‐induced embrittlement are analyzed. The use of Zn–Ni alloy coating, which is characterized by a higher melting temperature, is reviewed. The behavior of sol–gel hybrid coatings on hot stamped steels is discussed. The possible use of the recently developed Al–Zn alloy coatings, dual layer Zn–Al and Zn–Al–Mg coatings is also introduced. The application of Zn–Al–Mg post‐process galvanizing is also discussed. In each case, all available information related to the weldability, paintability, and corrosion resistance of the coating systems is also reported. Finally, the advantages and technical challenges associated with each type of coating are reviewed.
Mechanical Properties of (20–30)Mn12Cr(0.56–0.7)CN Corrosion Resistant Austenitic TWIP Steels
Authors: Mújica Roncery Lais , Weber Sebastian , Theisen Werner 
Published: March 2012

AbstractNew developed (20–30)Mn12Cr(0.56–0.7)CN TWIP steels developed from thermodynamic calculations exhibit great mechanical properties, such as high strength (1800 MPa UTS), deformability (80–100% elongation), toughness (300 J ISO‐V), and impact wear resistance equivalent to that of Hadfield steel. In addition, they exhibit corrosion resistance by passivation in aqueous acidic media. Microstructure examination by SEM and EBSD at different degrees of deformation reveals that twinning takes place and is responsible for the high cold‐work hardening of the steels. Stacking fault energy measurement of three different developed steels locates them in the range of 30–40 mJ m−2, being highly dependent on the N and Mn contents. Measurements carried out with digital image correlation indicate that at room temperature dynamic strain aging or Portevin–LeChatelier effect takes place. Measurements of impact toughness indicate that the steels have ductile to brittle transition at cryogenic temperatures as a consequence of the effect of nitrogen on the deformation mechanisms, resulting in a quasi‐cleavage fracture along the {111} planes at −196°C.
Breakthrough of traditional shear wall structure system—short‐leg wall structure system
Authors: Kui Dai 
Published: March 2012

SUMMARYTraditional shear wall structure has small space, is not flexible, causes waste of material and has bad economic efficiency. Through complete transformation of traditional shear wall, a new structure system has been created. The research and application of short‐leg shear wall structure calculation theory are based on the national codes, from which the short‐leg shear wall design principles are established. Traditional shear wall structure is discussed because of the world's first short‐leg shear wall structure design formation and development research. According to short‐leg shear wall force characteristics, horizontal displacement is divided into destructive storey drift and harmless storey drift; the formula for calculating the destructive storey drift is obtained, using destructive storey drift angle parameters and the change of main section height to control the deformation, to control structural rigidity and to ensure that the structural design attains excellence. Copyright © 2012 John Wiley & Sons, Ltd.
Proposal for a morphological classification of intraductal papillary neoplasm of the bile duct (IPN‐B)
Authors: Kato Hiroyuki , Tabata Masami , Azumi Yoshinori , Osawa Ichiro , Kishiwada Masashi , Hamada Takashi , Mizuno Shugo , Usui Masanobu , Sakurai Hiroyuki , Isaji Shuji 
Published: March 2012

AbstractPurposeWe propose a morphological classification of intraductal papillary neoplasm of the bile duct (IPN‐B).MethodsA retrospective analysis of 16 patients with IPN‐B who had undergone surgical resection was conducted. These 16 cases were classified into three types based on the primary lesion's anatomical location: branch duct type (6 cases), main duct type (5 cases) and mixed type (5 cases). In this paper we have analyzed the characteristics of IPN‐B according to our new classification.ResultsAll branch duct type IPN‐B was located in the left lobe and 5 of them were resected by left hepatectomy without extrahepatic bile duct resection (EBDR). On the other hand, all patients with main duct and mixed type IPN‐B underwent EBDR in addition to hepatectomy or pancreatoduodenectomy. Microscopically, 2 of 6 patients with branch duct type IPN‐B had no malignant component and, in the remaining 4 patients, cancer invasion was restricted to within the ductal wall. There were no cases of branch duct type IPN‐B with lymph node metastasis and superficial intraductal tumor spread. In contrast, all patients with main duct and mixed duct type IPN‐B had the malignant component. In all cases, lymph node metastasis was not observed, but superficial intraductal tumor spread was frequently found in extrahepatic bile duct lesions: 3 of main duct type and 3 of mixed type. Four patients with superficial intraductal spread had non‐curative resection due to a cancer‐positive ductal margin. Most patients with IPN‐B obtained a good prognosis, but two patients with a cancer‐positive ductal margin developed local recurrence.ConclusionsOur classification vividly reflects clinical and pathological characteristics of IPN‐B and is useful to determine appropriate surgical strategy.