bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gastric mucormycosis in a cat
Authors: Mavilio Emilio , Bottero Enrico 
Published: February 2025

Case summary This report describes a case of gastric mucormycosis in a young Ragdoll cat with a 5-day history of vomiting. Physical examination detected mild dehydration and tenderness was elicited on abdominal palpation. The results of blood work-up and radiographic study were unremarkable; however, abdominal ultrasonographic examination revealed multiple hyperechoic neoformations at the level of the pyloric antrum, which were confirmed on endoscopic examination. Non-septate hyphae of irregular diameter with a branched appearance were observed on cytology, and histological examination revealed severe diffuse necrotising and granulomatous gastritis with the presence of intralesional fungal hyphae indicative of mucormycosis, which was confirmed by PCR tests. Antifungal therapy with ketoconazole in addition to supportive treatment temporarily improved the clinical condition. Lethargy, fever and abdominal effusion developed in the following days. Cytological examination of abdominal fluid was compatible with septic peritonitis and, given the severity of the condition, euthanasia was opted by the owners. Post-mortem examination confirmed septic peritonitis resulting from perforation of the gastric wall at one of the neoformations of the pyloric antrum. Relevance and novel information To the authors’ knowledge, this is the first reported case of gastric mucormycosis in a cat. Previous literature includes a case of mucormycosis in a Persian cat affecting only the duodenum. In both the Persian cat and the cat described here, gastrointestinal mucormycosis disease progressed rapidly and was fatal.
How LGBTQ + adults’ experiences of multiple disadvantage impact upon their health and social care service pathways in the UK & Ireland: a scoping review
Authors: Adley Mark , O’Donnell Amy , Scott Stephanie 
Published: February 2025

Abstract

Background
Despite increased awareness of the significant health and healthcare inequalities experienced by minoritised groups, limited research considers the interaction of multiple domains of social disadvantage. This review therefore sought to explore how LGBTQ + adults’ experiences of homelessness, substance use, and criminal justice involvement impact upon their access to and use of health and social care services in the UK and Ireland.

Methods
A qualitative scoping review was conducted in accordance with the PRISMA-ScR framework. Electronic database and web searches identified 26 eligible peer-reviewed and grey literature documents published between 2010–2024. The data were charted, coded, and knowledge gaps identified.

Results
Data were coded thematically, clustered around the concept of normativity. Descriptive qualitative techniques were applied to explore how this was enacted and experienced. Synthesis across the literature identified experiences of discrimination and anticipated stigma that acted as barriers to accessing and engaging with services.

Conclusions
Structural normativity and the privilege afforded to hegemonic population groups impacted upon this population’s access to and use of services. The review adds depth and context to questions around the lack of visibility or engagement in services by LGBTQ + people with experience of disadvantage, and contributes to the wider literature on improving service access for marginalised, underserved, or disadvantaged communities.
Whole-exome sequencing identifies distinct genomic aberrations in eccrine porocarcinomas and poromas
Authors: Puttonen Maya , Almusa Henrikki , Böhling Tom , Koljonen Virve , Sihto Harri 
Published: February 2025

Abstract

Background
Eccrine porocarcinoma (EPC) is a rare malignant skin tumor arising from the eccrine gland. Investigations into the genomic landscape of EPC have uncovered potential drivers of its development and progression. However, there is limited information on the discrepancies between EPC and its benign counterpart, eccrine poroma (EP).

Methods
Formalin-fixed paraffin-embedded (FFPE) samples from 15 EPCs and 5 EPs were retrieved from Helsinki Biobank and Finnish Clinical Biobank Tampere. One EPC was found to be digital papillary adenocarcinoma in review of diagnoses. Whole-exome sequencing was used to conduct a comprehensive analysis to elucidate the genomic features of EPCs and EPs.

Results
There was general heterogeneity within EPCs and EPs, with discrepancies such as exclusive TP53, NCOR1, and CDKN2A mutations in EPCs and a higher mutational load in EPCs than in EPs. Furthermore, we identified alterations in pathways associated with cell adhesion and the extracellular matrix in EPCs, while pathways associated with ketone body and amino acid metabolism were altered in EPs. The MAPK and Ras signaling pathways were enriched in genes mutated only in EPCs.

Conclusions
EPCs and EPs are generally heterogeneous tumor entities with a few distinct discrepancies from each other. The findings from this study emphasize the need to further verify the roles of disrupted genes and pathways in the initiation and progression of EPCs and EPs.
CAR-macrophages targets CD26 to eliminate chronic myeloid leukemia stem cells
Authors: Guoyun Jiang , Yuefeng Qin , Zhenglan Huang , Zuowei Yuan , Hongyan Zhou , Ying Yuan , Wenli Feng 
Published: February 2025

Abstract

Background
Chronic myeloid leukemia stem cells (CML-LSCs), which exhibit resistance to tyrosine kinase inhibitors (TKIs), are the leading cause of treatment failure and recurrence in chronic myeloid leukemia (CML). This highlights the urgent need for novel therapies aimed at eliminating these CML-LSCs. Chimeric antigen receptor macrophages (CAR-M) not only perform phagocytosis on target cells but also function as antigen-presenting cells, thereby activating the anti-tumor immune response.CD26 (dipeptidyl peptidase 4, DPP IV) is abundantly expressed in CML-LSCs and functions as a tumor-specific antigen (TSA) in CAR-M treatment. The purpose of this study is to evaluate CAR-M’s efficacy in targeting CD26-positive CML cells and to develop a novel strategy for CML treatment.

Methods
CD26 CAR-M was constructed using mouse-derived macrophage Raw264.7 cells. CD26 was overexpressed in CML cell lines BP210 and BP210-T315I. The targeting phagocytosis of CAR-M was verified using confocal microscopy and flow cytometry. X-ray was used to eliminate the tumorigenicity of CAR-M, and the safety of CAR-M was verified through CCK-8, clone formation assays, and animal experiments. To assess the anti-leukemia ability of CAR-M in the CML mouse model, the survival, peripheral blood white blood cell counts, and CML cell infiltration in the liver, spleen, and bone marrow (BM) were measured. Additionally, CD26 CAR-THP1 was constructed, and its phagocytic ability against CD26-positive cells NCI-H2452 was confirmed by confocal microscopy.

Results
We successfully constructed CD26 CAR-M and validated its targeted phagocytosis of CD26-positive CML cells both in vitro and in vivo. The data indicate that CAR-M has higher phagocytic efficiency in CD26-positive CML cells than in CD26-negative cells. CAR-M-treated CML mice demonstrated extended survival and reduced CML invasion. In addition, CAR-THP1 demonstrated targeted phagocytosis of NCI-H2452 cells that normally express CD26.

Conclusion
This study demonstrates that CD26 CAR-M effectively targets and phagocytizes CD26-positive CML cells, implying that targeting CD26 with CAR-M could be a viable method for eradicating CML-LSCs. Furthermore, our discoveries illuminate the potential application of CAR-M in treating hematological malignancies.
Blood biomarker fingerprints in a cohort of patients with CHRNE-related congenital myasthenic syndrome
Authors: Della Marina Adela , Koutsoulidou Andrie , Natera-de Benito Daniel , Tykocinski Lars-Oliver , Tomazou Marios , Georgiou Kristia , Laner Andreas , Kölbel Heike , Nascimento Andres , Ortez Carlos , Abicht Angela , Thakur Basant Kumar , Lochmüller Hanns , Phylactou Leonidas A. , Ruck Tobias , Schara-Schmidt Ulrike , Kale Dipali , Hentschel Andreas , Roos Andreas 
Published: February 2025

Abstract
Mutations in CHRNE encoding the epsilon subunit of acetylcholine receptor result in impaired neuromuscular transmission and congenital myasthenic syndrome (CMS) with variying severity of symptoms. Although the pathophysiology is well-known, blood biomarker signatures enabling a patient-stratification are lacking. This retrospective two-center-study includes 19 recessive CHRNE-patients (AChR deficiency; mean age 14.8 years) from 13 families which were clinically characterized according to disease severity. 15 patients were classified as mildly and 4 patients as moderate to severely affected. Seven known pathogenic and one unreported variant (c.1032 + 2_1032 + 3delinsGT) were identified. Biomarker discovery was carried out on blood samples: proteomics was performed on white blood cells (WBC; n = 12) and on extracellular vesicles (EV) purified from serum samples (n = 7) in addition to amino acid profiling (n = 9) and miRNA screening (n = 18). For miRNA studies, 7 patients with other CMS-subtypes were moreover included. WBC-proteomics unveiled a significant increase of 7 and a decrease of 36 proteins. In silico studies of these proteins indicated affection of secretory granules and the extracellular space. Comparison across patients unveiled increase of two vesicular transport proteins (SCAMP2 and SNX2) in severely affected patients and indeed EV-proteomics revealed increase of 7 and decrease of 13 proteins. Three of these proteins (TARSH, ATRN & PLEC) are known to be important for synaptogenesis and synaptic function. Metabolomics showed decrease of seven amino acids/ amino acid metabolites (aspartic and glutamic acids, phosphoserine, amino adipate, citrulline, ornithine, and 1-methyhistidine). miRNA-profiling showed increase miR − 483 − 3p, miR-365a-3p, miR − 365b − 3p and miR-99a, and decrease of miR-4433b-3p, miR-6873-3p, miR-182-5p and let-7b-5p in CHRNE-patients whereas a comparison with other CMS subtypes showed increase of miR − 205 − 5p, miR − 10b − 5p, miR-125a-5p, miR-499-5p, miR-3120-5p and miR − 483 − 5p and decrease of miR − 1290. Our combined data introduce a molecular fingerprint on protein, metabolic and miRNA level with some of those playing different roles along the neuromuscular axis.
Economic freedom, economic sustainability, and herding behavior: Does the ubiquity of information communication technology matter?
Authors: Saadaoui Mallek Ray , Albaity Mohamed , Rahman Mahfuzur 
Published: February 2025

Abstract
This study investigates the impact of information communication technology (ICT), economic freedom, and economic sustainability on herding behavior, and examines whether the link between economic freedom and economic sustainability depends on ICT. Using quantile regression on a sample of seven Gulf Cooperation Council stock markets from 2004 to 2020, we find that ICT and economic freedom reduce herding in lower quantiles while promoting it in higher quantiles. Conversely, open market freedom and economic sustainability appear to correct herding in all quantiles. The results show that ICT reinforces the move away from herding seen with economic freedom for the transmission channel of ICT on herding. However, ICT plays a dual role in open market freedom and economic sustainability, weakening (strengthening) the curative effect of economic sustainability (open market freedom) in the lower quantiles and strengthening (weakening) it in higher quantiles. Thus, the highest impact in reducing herding exits through the combination of ICT and economic freedom is skewed to the lower quantiles with ICT-open market freedom and skewed to the upper quantiles with ICT-economic sustainability. These results offer substantial implications for policymakers.
Discovery of plasma proteins and metabolites associated with left ventricular cardiac dysfunction in pan-cancer patients
Authors: Lal Jessica C. , Fang Michelle Z. , Hussain Muzna , Abraham Abel , Tonegawa-Kuji Reina , Hou Yuan , Chung Mina K. , Collier Patrick , Cheng Feixiong 
Published: February 2025

Abstract

Background
Cancer-therapy related cardiac dysfunction (CTRCD) remains a significant cause of morbidity and mortality in cancer survivors. In this study, we aimed to identify differential plasma proteins and metabolites associated with left ventricular dysfunction (LVD) in cancer patients.

Methods
We analyzed data from 50 patients referred to the Cleveland Clinic Cardio-Oncology Center for echocardiograph assessment, integrating electronic health records, proteomic, and metabolomic profiles. LVD was defined as an ejection fraction ≤ 55% based on echocardiographic evaluation. Classification-based machine learning models were used to predict LVD using plasma metabolites and proteins as input features.

Results
We identified 13 plasma proteins (P < 0.05) and 14 plasma metabolites (P < 0.05) associated with LVD. Key proteins included markers of inflammation (ST2, TNFRSF14, OPN, and AXL) and chemotaxis (RARRES2, MMP-2, MEPE, and OPN). Notably, sex-specific associations were observed, such as uridine (P = 0.003) in males. Furthermore, metabolomic features significantly associated with LVD included 1-Methyl-4-imidazoleacetic acid (P = 0.015), COL1A1 (P = 0.009), and MMP-2 (P = 0.016), and pointing to metabolic shifts and heightened inflammation in patients with LVD.

Conclusion
Our findings suggest that circulating metabolites may non-invasively detect clinical and molecular differences in patients with LVD, providing insights into underlying disease pathways and potential therapeutic targets.
Physical activity and sedentary behaviour amongst children with obesity - exploring cross-sectional associations between child and parent
Authors: Lundh Hannah , Arvidsson Daniel , Greven Christian , Fridolfsson Jonatan , Börjesson Mats , Boman Charlotte , Lauruschkus Katarina , Lundqvist Stefan , Melin Karin , Bernhardsson Susanne 
Published: February 2025

Abstract

Background
Physical activity (PA) in childhood is critical for establishing a healthy lifestyle across the lifespan, particularly to treat and prevent obesity. This study aimed to explore PA and sedentary behaviour (SED) in 6–12-year-old children with obesity and their parents, and possible associations in these behaviours between children and parents.

Methods
Children referred to outpatient paediatric healthcare for obesity treatment and one of their parents wore accelerometers (Axivity) on their hip during seven consecutive days. Accelerometer data were processed using the 10 Hz frequency extended method. Correlations between child and parent PA and SED, respectively, were analysed using intra-class correlation coefficient.

Results
Thirty-nine children (19 female) and 38 parents (20 female) were included. The mean age of the children was 9.7 years (SD 2.0) and the mean parent age was 42.2 years (SD 6.1). The mean child BMI-SDS was 3.0 (SD 0.4). Fifty-seven % of the parents were born in Sweden, 16% in other European countries, and 27% outside Europe. Children spent an average of 9.8 h/day in SED, while parents spent an average of 12.3 h/day. The mean daily time spent in low-intensity PA was 3.9 h for children and 3.4 h for parents, while moderate-to-vigorous intensity PA averaged 0.7 h/day for children and 0.3 h/day for parents. Only six of the children (15%) reached the recommended minimum of 60 min of moderate-to-vigorous intensity PA per day and only two parents (5%) reached the recommended weekly minimum of 150 min of moderate intensity PA. Child and parent SED was significantly correlated, although the correlation was weak (ICC 0.14; p = 0.017). No statistically significant correlations were found for any of the analysed PA intensity levels.

Conclusions
The findings indicate an association between children’s and parents’ SED in this sample of school-aged children with obesity, while no association was observed in PA behaviour. Generalisability of our findings is limited and more research is needed– in larger samples, other settings, and using longitudinal designs– to better understand the potential links between the PA patterns of children with obesity and that of their parents.
Using Adhesive Strategies in Molar Rehabilitation after Endodontic Treatment: A Case Series
Authors: Julian Nikhil , Walia Apoorva , Shetty Tejaswini , Mala Kundabala , Thomas Manuel S 
Published: February 2025

The shift towards conservative, adhesive, post-endodontic ceramic restorations represents a significant advance in restorative dentistry. By focusing on minimally invasive techniques and the use of adhesive materials, clinicians can achieve better long-term outcomes in terms of tooth preservation, function and aesthetics. The key lies in the careful assessment of each case, ensuring that the chosen restoration aligns with the principles of biomimetic dentistry, which seeks to restore the tooth in a manner that closely mimics its natural form and function. The purpose of this case series is to underscore the significance of adhesive post-endodontic ceramic restorations in the rehabilitation of the form, function and aesthetics of mutilated posterior teeth. CPD/Clinical Relevance: The use of conservative posterior indirect adhesive restorations can enhance the long-term survival of endodontically treated teeth.
Acute Sialadenitis of the Parotid Gland owing to Candidal Infection
Authors: Orion O'Brien , Crozier Rebecca 
Published: February 2025

This report describes the case of a patient with Sjögren's syndrome who presented with acute suppurative parotitis. A microbiological test of the purulent discharge obtained from the right parotid duct revealed the condition was secondary to acute Candida infection of the parotid gland. Candida infection of the salivary glands is typically only reported in immunocompromised patients and those with diabetes. In this case, the infection persisted after the initial flucloxacillin prescription, but was later resolved after the patient was given fluconazole for 7 days. CPD/Clinical Relevance: This case highlights relevant considerations when patients with a high risk of oral candidosis present with acute sialadenitis
Grey Areas in Restorative Dentistry: Part 4. Mind the Gap 1: The Radiographic Space between Restoration and Tooth
Authors: Caplin Robert L 
Published: February 2025

Radiopacities and radiolucencies beneath restorations provide the dental practitioner with diagnostic challenges as to the nature of such findings. Understanding the possible aetiologies of these radiographic appearances is essential for their subsequent management to avoid unnecessary intervention. CPD/Clinical Relevance: Radiographic changes around restorations are a relatively common finding and require the practitioner to decide whether to supervise or intervene.
A flat-rate income tax on revenue recorded as an effective form of taxation on the economic activities of individuals
Authors: Lucha Sylwia 
Published: February 2025

The article presents an analysis of the form of income taxation in Poland which is a lump sum recorded in terms of its conduct, as well as the construction of this variant of settlement with the tax office. The types of taxation on the basis of personal income tax have been men-tioned, which begins the criteria and restrictions that the income tax payer must meet in order to be able to settle with the chosen form. The example company, its analysis and example of settlement by means of a recorded lump sum of this particular enterprise was given.Â Â
The central bank and its role in shaping and managing the monetary policy of the state
Authors: Lucha Daria 
Published: February 2025

The article presents the role of the central bank in shaping and managing the monetary policy of the country. The central bank plays a special role in the state. It is an institution that is important for the country's economy. It is responsible for conducting monetary and exchange rate policy in the state. The central bank has a fundamental influence on the level of stability of the banking system of a given country and has a monopoly in the scope of central money emission, which is necessary for proper functioning of commercial banks.Â Â Â
Impact of large class on teaching learning at the primary school of Bangladesh
Authors: A. K. M. Obaydullah Dr. , Shahnewaz Md. 
Published: February 2025

Each Large classes teachers faces challenges everyday diversity of scholars , lack of flexibility, class environment management, difficulty of setting and enforcing classroom behavior, minimum attention to students, limited monitoring of students' learning and difficulty in engaging students to activities. The main assumption of this study is that effective teaching and producing learning is critically constrained by the large size of classes. Therefore the students' perception about large classes is negative. This research is to interact during a study of the effectiveness of educational science for big classes. The baseline of our study is predicated on data that has been collected from students' survey. The study was conducted throughout the 40 government primary school in Gazipur district of Bangladesh. During this study are observed the students' expectations, motivation, own perception on performance, views and preferences about the delivery of the lecture and therefore the learning. It was designed the study to detect accurately the maximum amount as possible students' attitude towards learning. Each student's responses, like the students own perception has been correlated to real performance as measured, weekly class work, quizzes, tutorials In this study will focuses other solutions to the above obstacles to conducting an efficient learning environment. The lecturing time and the mode of teaching both are investigated, and reviewed as a possible solution the issues encountered during the lecture. It compared the findings to the prevailing literature and other teachers' experiences. Studying studentsâ€™ experience is sort of challenging. It may be used as a top quality indicator additionally to the quality in education. Evaluating studentsâ€™ experience, need and expectations can cause improvements in teaching performance and achieving learning outcomes. Finally, there'll be a discussion of the problems raised and therefore the implications of those for various stakeholders in developing countries.Â Â
The status quo of building codes and construction practices in Somaliland: practitioners' perceptions
Authors: A. Sheikh Ahmed , F. Fakunle Funke , A. Fashina Adebayo 
Published: February 2025

For several years, building codes have been perceived as one of the significant ways of establishing building quality, environmental protection, public health and safety, and energy efficiency in the building and construction industry. Conversely, some least developing countries like Somaliland are yet to adopt and enforce the current building codes and as such allowing the construction of buildings that do not meet the minimum international standards on public health and safety. Consequently, this paper seeks to review the overall and genuine picture about Somaliland 's building traditions, history, and advancement alongside the construction practices in Somaliland. In addition, unstructured interviews were held with some top governmental officials from the Ministry of Housing and Public Works, Ministry of Environment & Rural Development, the Somaliland Fire Agency, and Hargeisa Regional Court to carefully explore the status quo of building codes in Somaliland. This was achieved by generating qualitative data via the use of open-ended questions. The findings from the study revealed that there are currently no building codes or regulations in Somaliland. Meaning that the building industry is presently self-governed by the owners and the construction professionals. The study concludes that in spite of the absent of building codes and regulations, there have been a rapid development in the building and construction industry and the increase of mid-rise building of four to six story buildings in Somaliland.Â Â