bluuebunny/crossref_metadata_2025_split huggingface dataset:
Administration and effects of beta blockers and oxandrolone in severely burned adults: a post hoc analysis of the RE-ENERGIZE trial
Authors: Hundeshagen Gabriel , Blears Elisabeth , Mertin Viktoria , Day Andrew G , Palackic Alen , Tapking Christian , Haug Valentin , Kneser Ulrich , Bliesener Björn , Panayi Adriana C , Aballay Ariel , Depret Francois , Stoppe Christian , Heyland Daren K 
Published: April 2024

Abstract

Background
Prospective randomized trials in severely burned children have shown the positive effects of oxandrolone (OX), beta blockers (BB) and a combination of the two (BBOX) on hypermetabolism, catabolism and hyperinflammation short- and long-term post-burn. Although data on severely burned adults are lacking in comparison, BB, OX and BBOX appear to be commonly employed in this patient population. In this study, we perform a secondary analysis of an international prospective randomized trial dataset to provide descriptive evidence regarding the current utilization patterns and potential treatment effects of OX, BB and BBOX.

Methods
The RE-ENERGIZE (RandomizEd Trial of ENtERal Glutamine to minimIZE Thermal Injury, NCT00985205) trial included 1200 adult patients with severe burns. We stratified patients according to their receipt of OX, BB, BBOX or none of these drugs (None) during acute hospitalization. Descriptive statistics describe the details of drug therapy and unadjusted analyses identify predisposing factors for drug use per group. Association between OX, BB and BBOX and clinical outcomes such as time to discharge alive and 6-month mortality were modeled using adjusted multivariable Cox regressions.

Results
More than half of all patients in the trial received either OX (n = 138), BB (n = 293) or BBOX (n = 282), as opposed to None (n = 487, 40.6%). Per study site and geographical region, use of OX, BB and BBOX was highly variable. Predisposing factors for the use of OX, BB and BBOX included larger total body surface area (TBSA) burned, higher acute physiology and chronic health evaluation (APACHE) II scores on admission and younger patient age. After adjustment for multiple covariates, the use of OX was associated with a longer time to discharge alive [hazard ratio (HR) 0.62, confidence interval (CI) (0.47–0.82) per 100% increase, p = 0.001]. A higher proportion of days on BB was associated with lower in-hospital-mortality (HR: 0.5, CI 0.28–0.87, p = 0.015) and 6-month mortality (HR: 0.44, CI 0.24–0.82, p = 0.01).

Conclusions
The use of OX, BB and BBOX is common within the adult burn patient population, with its use varying considerably across sites worldwide. Our findings found mixed associations between outcomes and the use of BB and OX in adult burn patients, with lower acute and 6-month-mortality with BB and longer times to discharge with OX. Further research into these pharmacological modulators of the pathophysiological response to severe burn injury is indicated.
Applications of mesenchymal stem cell-exosome components in wound infection healing: new insights
Authors: Fakouri Arshia , Razavi Zahra-Sadat , Mohammed Adil Tawfeeq , Hussein Abbas Hameed Abdul , Afkhami Hamed , Hooshiar Mohammad Hosseini 
Published: April 2024

Abstract
The healing process at a wound is made up of many types of cells, growth factors, the extracellular matrix, nerves and blood vessels all interacting with each other in complex and changing ways. Microbial colonization and proliferation are possible at the place of injury, which makes infection more likely. Because of this, any cut has a chance of getting an infection. Researchers have found that wound infections make patients more upset and cost the healthcare system a lot of money. Surgical site infections happen a lot to people who have recently had surgery. This study shows that such surgical infection is linked to a high rate of illness and death. This is shown by the fact that 25% of patients get serious sepsis and need to be transferred to an intensive care unit. In both animal models and people, mesenchymal stem cells (MSCs) play an active role in all stages of wound healing and have positive effects. Exosomes are one of the main things MSCs release. They have effects that are similar to those of the parent MSCs. Various effector proteins, messenger RNA and microRNAs can be transported by extracellular vesicles to control the activity of target cells. This has a big impact on the healing process. These results suggest that using MSC-exosomes as a new type of cell-free therapy could be a better and safer option than whole cell therapy. This review is mostly about how to use parts of MSC-exosomes to help wound infections heal.
A Human Immunodeficiency Virus Type 1 Risk Assessment Tool for Women Aged 15–49 Years in African Countries: A Pooled Analysis Across 15 Nationally Representative Surveys
Authors: Rosenberg Nora E , Shook-Sa Bonnie E , Young Amber M , Zou Yating , Stranix-Chibanda Lynda , Yotebieng Marcel , Sam-Agudu Nadia A , Phiri Sam J , Mutale Wilbroad , Bekker Linda-Gail , Charurat Manhattan E , Moyo Sizulu , Zuma Khangelani , Justman Jessica , Hudgens Michael G , Chi Benjamin H 
Published: April 2024

Abstract

Background
Women in Africa disproportionately acquire human immunodeficiency virus type 1 (HIV-1). Understanding which women are most likely to acquire HIV-1 can guide focused prevention with preexposure prophylaxis (PrEP). Our objective was to identify women at the highest risk of HIV-1 and estimate PrEP efficiency at different sensitivity levels.

Methods
Nationally representative data were collected from 2015 through 2019 from 15 population-based household surveys. This analysis included women aged 15–49 who tested HIV-1 seronegative or had recent HIV-1. Least absolute shrinkage and selection operator regression models were fit with 28 variables to predict recent HIV-1. Models were trained on the full population and internally cross-validated. Performance was evaluated using area under the receiver operating characteristic curve (AUC), sensitivity, and number needed to treat (NNT) with PrEP to avert 1 infection.

Results
Among 209 012 participants, 248 had recent HIV-1 infection, representing 118 million women and 402 000 (95% confidence interval [CI], 309 000–495 000) annual infections. Two variables were retained: living in a subnational area with high HIV-1 viremia and having a sexual partner living outside the home. The full-population AUC was 0.80 (95% CI, .76–.84); cross-validated AUC was 0.79 (95% CI, .75–.84). At 33% sensitivity, 130 000 cases could be averted if 7.9 million women were perfectly adherent to PrEP; NNT would be 61. At 67% sensitivity, 260 000 cases could be averted if 25.1 million women were perfectly adherent; NNT would be 96.

Conclusions
This risk assessment tool was generalizable, predictive, and parsimonious with trade-offs between reach and efficiency.
Prevalence of chronic kidney disease in France: methodological considerations and pitfalls with the use of Health claims databases
Authors: Couchoud Cécile , Raffray Maxime , Lassalle Mathilde , Duisenbekov Zhanibek , Moranne Olivier , Erbault Marie , Lazareth Hélène , Parmentier Cyrielle , Guebre-Egziabher Fitsum , Hamroun Aghiles , Metzger Marie , Mansouri Imene , Goldberg Marcel , Zins Maris , Bayat-Makoei Sahar , Kab Sofiane 
Published: April 2024

ABSTRACT

Background
Health policy-making require careful assessment of chronic kidney disease (CKD) epidemiology to develop efficient and cost-effective care strategies. The aim of the present study was to use the RENALGO-EXPERT algorithm to estimate the global prevalence of CKD in France.

Methods
An expert group developed the RENALGO-EXPERT algorithm based on healthcare consumption. This algorithm has been applied to the French National Health claims database (SNDS), where no biological test findings are available to estimate a national CKD prevalence for the years 2018–2021. The CONSTANCES cohort (+219 000 adults aged 18–69 with one CKD-EPI eGFR) was used to discuss the limit of using health claims data.

Results
Between 2018 and 2021, the estimated prevalence in the SNDS increased from 8.1% to 10.5%. The RENALGO-EXPERT algorithm identified 4.5% of the volunteers in the CONSTANCES as CKD. The RENALGO-EXPERT algorithm had a positive predictive value of 6.2% and negative predictive value of 99.1% to detect an eGFR&lt;60 ml/min/1.73 m². Half of 252 false positive cases (ALGO+, eGFR &gt; 90) had been diagnosed with kidney disease during hospitalization, and the other half based on healthcare consumption suggestive of a ‘high-risk’ profile; 95% of the 1661 false negatives (ALGO−, eGFR &lt; 60) had an eGFR between 45 and 60 ml/min, half had medication and two-thirds had biological exams possibly linked to CKD. Half of them had a hospital stay during the period but none had a diagnosis of kidney disease.

Conclusions
Our result is in accordance with other estimations of CKD prevalence in the general population. Analysis of diverging cases (FP and FN) suggests using health claims data have inherent limitations. Such an algorithm can identify patients whose care pathway is close to the usual and specific CKD pathways. It does not identify patients who have not been diagnosed or whose care is inappropriate or at early stage with stable GFR.
Incidence and predictors of thermal oesophageal and vagus nerve injuries in Ablation Index-guided high-power-short-duration ablation of atrial fibrillation: a prospective study
Authors: Wolff Charlotte , Langenhan Katharina , Wolff Marc , Efimova Elena , Zachäus Markus , Darma Angeliki , Dinov Borislav , Seewöster Timm , Nedios Sotirios , Bertagnolli Livio , Wolff Jan , Paetsch Ingo , Jahnke Cosima , Bollmann Andreas , Hindricks Gerhard , Bode Kerstin , Halm Ulrich , Arya Arash 
Published: April 2024

Abstract

Aims
High-power-short-duration (HPSD) ablation is an effective treatment for atrial fibrillation but poses risks of thermal injuries to the oesophagus and vagus nerve. This study aims to investigate incidence and predictors of thermal injuries, employing machine learning.

Methods and results
A prospective observational study was conducted at Leipzig Heart Centre, Germany, excluding patients with multiple prior ablations. All patients received Ablation Index-guided HPSD ablation and subsequent oesophagogastroduodenoscopy. A machine learning algorithm categorized ablation points by atrial location and analysed ablation data, including Ablation Index, focusing on the posterior wall. The study is registered in clinicaltrials.gov (NCT05709756). Between February 2021 and August 2023, 238 patients were enrolled, of whom 18 (7.6%; nine oesophagus, eight vagus nerve, one both) developed thermal injuries, including eight oesophageal erythemata, two ulcers, and no fistula. Higher mean force (15.8 ± 3.9 g vs. 13.6 ± 3.9 g, P = 0.022), ablation point quantity (61.50 ± 20.45 vs. 48.16 ± 19.60, P = 0.007), and total and maximum Ablation Index (24 114 ± 8765 vs. 18 894 ± 7863, P = 0.008; 499 ± 95 vs. 473 ± 44, P = 0.04, respectively) at the posterior wall, but not oesophagus location, correlated significantly with thermal injury occurrence. Patients with thermal injuries had significantly lower distances between left atrium and oesophagus (3.0 ± 1.5 mm vs. 4.4 ± 2.1 mm, P = 0.012) and smaller atrial surface areas (24.9 ± 6.5 cm2 vs. 29.5 ± 7.5 cm2, P = 0.032).

Conclusion
The low thermal lesion’s rate (7.6%) during Ablation Index-guided HPSD ablation for atrial fibrillation is noteworthy. Machine learning based ablation data analysis identified several potential predictors of thermal injuries. The correlation between machine learning output and injury development suggests the potential for a clinical tool to enhance procedural safety.
Charting the course of pinniped evolution: insights from molecular phylogeny and fossil record integration
Authors: Park Travis , Burin Gustavo , Lazo-Cancino Daniela , Rees Joseph P G , Rule James P , Slater Graham J , Cooper Natalie 
Published: April 2024

Abstract
Pinnipeds (seals, sea lions, walruses, and their fossil relatives) are one of the most successful mammalian clades to live in the oceans. Despite a well-resolved molecular phylogeny and a global fossil record, a complete understanding of their macroevolutionary dynamics remains hampered by a lack of formal analyses that combine these 2 rich sources of information. We used a meta-analytic approach to infer the most densely sampled pinniped phylogeny to date (36 recent and 93 fossil taxa) and used phylogenetic paleobiological methods to study their diversification dynamics and biogeographic history. Pinnipeds mostly diversified at constant rates. Walruses, however, experienced rapid turnover in which extinction rates ultimately exceeded speciation rates from 12 to 6 Ma, possibly due to changing sea levels and/or competition with otariids (eared seals). Historical biogeographic analyses, including fossil data, allowed us to confidently identify the North Pacific and the North Atlantic (plus or minus Paratethys) as the ancestral ranges of Otarioidea (eared seals + walrus) and crown phocids (earless seals), respectively. Yet, despite the novel addition of stem pan-pinniped taxa, the region of origin for Pan-Pinnipedia remained ambiguous. These results suggest further avenues of study in pinnipeds and provide a framework for investigating other groups with substantial extinct and extant diversity.
Multiomics data integration, limitations, and prospects to reveal the metabolic activity of the coral holobiont
Authors: Williams Amanda 
Published: April 2024

Abstract
Since their radiation in the Middle Triassic period ∼240 million years ago, stony corals have survived past climate fluctuations and five mass extinctions. Their long-term survival underscores the inherent resilience of corals, particularly when considering the nutrient-poor marine environments in which they have thrived. However, coral bleaching has emerged as a global threat to coral survival, requiring rapid advancements in coral research to understand holobiont stress responses and allow for interventions before extensive bleaching occurs. This review encompasses the potential, as well as the limits, of multiomics data applications when applied to the coral holobiont. Synopses for how different omics tools have been applied to date and their current restrictions are discussed, in addition to ways these restrictions may be overcome, such as recruiting new technology to studies, utilizing novel bioinformatics approaches, and generally integrating omics data. Lastly, this review presents considerations for the design of holobiont multiomics studies to support lab-to-field advancements of coral stress marker monitoring systems. Although much of the bleaching mechanism has eluded investigation to date, multiomic studies have already produced key findings regarding the holobiont’s stress response, and have the potential to advance the field further.
Variation in processes of care for total hip arthroplasty across high-income countries
Authors: Skopec Laura , Berenson Robert A , Simon Benedikt , Papanicolas Irene 
Published: April 2024

Abstract
Total hip arthroplasty (THA) is among the most commonly performed elective surgeries in high-income countries, and wait times for THA have frequently been cited by US commentators as evidence that countries with universal insurance programs or national health systems “ration” care. This novel qualitative study explores processes of care for hip replacement in the United States and 6 high-income countries with a focus on eligibility, wait times, decision-making, postoperative care, and payment policies. We found no evidence of rationing or government interference in decision-making across high-income countries. Compared with the 6 other high-income countries in our study, the United States has developed efficient care processes that often allow for a same-day discharge. In contrast, THA patients in Germany stay in the hospital 7–9 days and receive 2–3 weeks of inpatient rehabilitation. However, the payment per THA in the United States remains far above other countries, despite far fewer inpatient days.
Chromosome-level genome assembly and population genomics reveals crucial selection for subgynoecy development in chieh-qua
Authors: Wang Min , Cao Zhenqiang , Jiang Biao , Wang Kejian , Xie Dasen , Chen Lin , Shi Shaoqi , Yang Songguang , Lu Hongwei , Peng Qingwu 
Published: April 2024

Abstract
Chieh-qua is an important cucurbit crop and very popular in South China and Southeast Asia. Despite its significance, its genetic basis and domestication history are unclear. In this study, we have successfully generated a chromosome-level reference genome assembly for the chieh-qua ‘A36’ using a hybrid assembly strategy that combines PacBio long reads and Illumina short reads. The assembled genome of chieh-qua is approximately 953.3 Mb in size and is organized into 12 chromosomes, with contig N50 of 6.9 Mb and scaffold N50 of 68.2 Mb. Notably, the chieh-qua genome is comparable in size to the wax gourd genome. Through gene prediction analysis, we have identified a total of 24 593 protein-coding genes in the A36 genome. Additionally, approximately 56.6% (539.3 Mb) of the chieh-qua genome consists of repetitive sequences. Comparative genome analysis revealed that chieh-qua and wax gourd are closely related, indicating a close evolutionary relationship between the two species. Population genomic analysis, employing 129 chieh-qua accessions and 146 wax gourd accessions, demonstrated that chieh-qua exhibits greater genetic diversity compared to wax gourd. We also employed the GWAS method to identify related QTLs associated with subgynoecy, an interested and important trait in chieh-qua. The MYB59 (BhiCQ0880026447) exhibited relatively high expression levels in the shoot apex of four subgynoecious varieties compared with monoecious varieties. Overall, this research provides insights into the domestication history of chieh-qua and offers valuable genomic resources for further molecular research.
Comparative transcriptome and functional analyses provide insights into the key factors regulating shoot regeneration in highbush blueberry
Authors: Omori Masafumi , Yamane Hisayo , Tao Ryutaro 
Published: April 2024

Abstract
Establishing an efficient plant regeneration system is a crucial prerequisite for genetic engineering technology in plants. However, the regeneration rate exhibits considerable variability among genotypes, and the key factors underlying shoot regeneration capacity remain largely elusive. Blueberry leaf explants cultured on a medium rich in cytokinins exhibit direct shoot organogenesis without prominent callus formation, which holds promise for expediting genetic transformation while minimizing somatic mutations during culture. The objective of this study is to unravel the molecular and genetic determinants that govern cultivar-specific shoot regeneration potential in highbush blueberry (Vaccinium corymbosum L.). We conducted comparative transcriptome analysis using two highbush blueberry genotypes: ‘Blue Muffin’ (‘BM’) displaying a high regeneration rate (&gt;80%) and ‘O’Neal’ (‘ON’) exhibiting a low regeneration rate (&lt;10%). The findings revealed differential expression of numerous auxin-related genes; notably, ‘BM’ exhibited higher expression of auxin signaling genes compared to ‘ON’. Among blueberry orthologs of transcription factors involved in meristem formation in Arabidopsis, expression of VcENHANCER OF SHOOT REGENERATION (VcESR), VcWUSCHEL (VcWUS), and VcCUP-SHAPED COTYLEDON 2.1 were significantly higher in ‘BM’ relative to ‘ON’. Exogenous application of auxin promoted regeneration, as well as VcESR and VcWUS expression, whereas inhibition of auxin biosynthesis yielded the opposite effects. Overexpression of VcESR in ‘BM’ promoted shoot regeneration under phytohormone-free conditions by activating the expression of cytokinin- and auxin-related genes. These findings provide new insights into the molecular mechanisms underlying blueberry regeneration and have practical implications for enhancing plant regeneration and transformation techniques.
Independent Transitions to Freshwater Environments Promote Phenotypic Divergence, Not Convergence, in Stingrays
Authors: Magnuson A , Dean M N , Weaver J C , Fontenelle J P , Lovejoy N R , Kolmann M A 
Published: April 2024

Synopsis
Instances of convergent or parallel evolution provide a potent model system for exploring contingency and determinism in evolutionary biology. Likewise, the multiple, independent habitat transitions from saltwater to freshwater biomes offer opportunities for studying convergent evolution within and among different vertebrate lineages. For example, stingrays have invaded freshwater habitats multiple times across different continents, sometimes even several times within the same clade (e.g., Dasyatidae). We evaluated the frequency of saltwater–freshwater invasions in stingrays, compared ecological and phenotypic diversification among freshwater and saltwater lineages, and assessed the degree of convergence among freshwater species. Despite not being morphologically distinct from saltwater stingrays, freshwater stingrays do expand the margins of stingray morphological diversity. According to our data, trophic specialists occupied non-overlapping regions of morphospace, with piscivores and molluscivores being distinct from other diet guilds. Freshwater stingrays as a group did not strongly converge morphologically, neither did freshwater rays from different lineages, which shared similar niches. These findings could be explained by there not being enough time for convergence to occur among more ancient and more recent freshwater lineages. Alternatively, the different ancestral bauplans of various freshwater ray lineages and weak selection on optimal phenotypes could promote contingency in the form of evolution along paths of least resistance.
Learning from Civil Society Actors in Turkey: Using Transitional Justice in an Ongoing Conflict
Authors: Alici Nisan 
Published: April 2024

ABSTRACT∞
This article examines the role of civil society in the implementation of transitional justice in Turkey’s ongoing Kurdish conflict, which is characterized by historical state violence and a lack of official transitional justice processes. Drawing on 24 interviews, the research reveals the evolution of civil society engagement from its peak during the 2013–2015 peace process to its decline under increasing authoritarianism. The research argues that, despite challenges, civil society retains the potential to drive incremental change in the absence of formal transitional justice mechanisms. However, the authoritarian regime has significantly curtailed its capacity, creating a limited civic space for transitional justice. The article highlights the need for stronger links between civil society in Turkey and international transitional justice scholarship. Such collaboration could empower activists, enabling them to overcome challenges and increase their effectiveness. Turkey offers a unique case for transitional justice scholars and practitioners to learn from civil society actors committed to pursuing justice.
A Construction of Deformations to General Algebras
Authors: Bowman David , Puljić Dora , Smoktunowicz Agata 
Published: April 2024

Abstract
One of the questions investigated in deformation theory is to determine to which algebras can a given associative algebra be deformed. In this paper we investigate a different but related question, namely: for a given associative finite-dimensional ${\mathbb{C}}$-algebra $A$, find algebras $N$, which can be deformed to $A$. We develop a simple method that produces associative and flat deformations to investigate this question. As an application of this method we answer a question of Michael Wemyss about deformations of contraction algebras.