bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Phosphatase Inhibitor Menadione (Vitamin K3) Protects Cells from EGFR Inhibition by Erlotinib and Cetuximab
Authors: Perez-Soler Roman , Zou Yiyu , Li Tianhong , Ling Yi He 
Published: September 2011

Abstract
Purpose: Skin toxicity is the main side effect of epidermal growth factor receptor (EGFR) inhibitors, often leading to dose reduction or discontinuation. We hypothesized that phosphatase inhibition in the skin keratinocytes may prevent receptor dephosphorylation caused by EGFR inhibitors and be used as a new potential strategy for the prevention or treatment of this side effect.
Experimental Design: Menadione (Vitamin K3) was used as the prototype compound to test our hypothesis. HaCat human skin keratinocyte cells and A431 human squamous carcinoma cells were used. EGFR inhibition was measured by Western blotting and immunofluorescence. Phosphatase inhibition and reactive oxygen species (ROS) generation were measured by standard ELISA and fluorescence assays.
Results: Menadione caused significant and reversible EGFR activation in a dose-dependent manner starting at nontoxic concentrations. EGFR activation by menadione was associated with reversible protein tyrosine phosphatase inhibition, which seemed to be mediated by ROS generation as exposure to antioxidants prevented both menadione-induced ROS generation and phosphatase inhibition. Short-term coincubation of cells with nontoxic concentrations of menadione and the EGFR inhibitors erlotinib or cetuximab prevented EGFR dephosphorylation. Seventy-two–hour coincubation of cells with the highest nontoxic concentration of menadione and erlotinib provided for a fourfold cell growth inhibitory protection in HaCat human keratinocyte cells.
Conclusions: Menadione at nontoxic concentrations causes EGFR activation and prevents EGFR dephosphorylation by erlotinib and cetuximab. This effect seems to be mediated by ROS generation and secondary phosphatase inhibition. Mild oxidative stress in skin keratinocytes by topical menadione may protect the skin from the toxicity secondary to EGFR inhibitors without causing cytotoxicity. Clin Cancer Res; 17(21); 6766–77. ©2011 AACR.
Lifestyle Factors in Relation to Heart Failure Among Finnish Men and Women
Authors: Wang Yujie , Tuomilehto Jaakko , Jousilahti Pekka , Antikainen Riitta , Mähönen Markku , Katzmarzyk Peter T. , Hu Gang 
Published: September 2011

Background—
The role of lifestyle factors in explaining the risk of heart failure (HF) is not properly established.

Methods and Results—

The national population-based study included 18 346 Finnish men and 19 729 women who were 25 to 74 years of age and free of HF at baseline. Multivariable Cox proportional hazards regression models was used to examine the association between lifestyle factors (smoking, body mass index [BMI], physical activity, vegetable consumption, fruit consumption, and alcohol consumption) and HF risk. During a median follow-up of 14.1 years (interquartile range, 5.9 to 20.9 years), 638 men and 445 women developed HF. Fruit consumption and alcohol consumption were dropped out of the analyses because no significant associations with the risk of HF were found. When 4 modifiable lifestyle factors (smoking, BMI, physical activity, and vegetable intake) were included in the analysis, the multivariable-adjusted (age, education, systolic blood pressure, total cholesterol, and histories of myocardial infarction, valvular heart disease, diabetes and using antihypertensive drugs) hazard ratios (HRs) of HF associated with engaging in 0, 1, 2, 3, and 4 healthy lifestyle factors were 1.00, 0.69 (95% confidence interval [CI], 0.54 to 0.87), 0.45 (95% CI, 0.35 to 0.58), 0.34 (95% CI, 0.25 to 0.46), and 0.31 (95% CI, 0.17 to 0.56) (
P
<0.001 for trend) for men, and 1.00, 0.53 (95% CI, 0.33 to 0.85), 0.42 (95% CI, 0.26 to 0.67), 0.24 (95% CI, 0.14 to 0.39), and 0.19 (95% CI, 0.09 to 0.40) (
P
<0.001 for trend) for women, respectively.

Conclusions—
The present study demonstrates an inverse association between healthy lifestyle patterns and the risk of HF in Finnish men and women.
Cocaine-induced Impulsive Choices Are Accompanied by Impaired Delay-dependent Anticipatory Activity in Basolateral Amygdala
Authors: Zuo Yanfang , Wang Xinsheng , Cui Cailian , Luo Fei , Yu Peng , Wang XueWei 
Published: September 2011

Abstract
Addicts and drug-experienced animals have decision-making deficits in delayed reinforcement choice task, in which they prefer small immediate rewards over large delayed rewards. Here, we show evidence that this deficit is accompanied by changed coding of delay length in the basolateral amygdala (BLA). A subset of neurons in BLA demonstrated delay-dependent anticipatory activity (either increase or decrease as a function of delay to reward) in naive rats. After 30 days of withdrawal from chronic cocaine treatment (30 mg/kg/day for 10 days ip), the proportion of delay-dependent anticipatory neurons reduced, whereas delay-dependent activity in response to elapsed delay after reward delivery increased, both in the proportion of delay-dependent neurons and in the extent of delay dependence. Cocaine exposure increased, instead of decreased, BLA neuronal expectation for different reward magnitudes. These results indicate that BLA is critical for representing and maintaining the information of delayed reward before its delivery, and cocaine exposure may affect decision-making by impairing perception of delay instead of the ability to assess the differences in reward size.
In vitro nematicidal activity of a garlic extract and salicylaldehyde on the potato cyst nematode, Globodera pallida
Authors: Danquah Wiseborn B. , Back Matthew A. , Grove Ivan G. , Haydock Patrick P.J. 
Published: September 2011

AbstractThe in vitro nematicidal effects of an aqueous garlic extract, salicylaldehyde, a nonylphenol ethoxylate surfactant and a formulation containing these constituents were evaluated against the potato cyst nematode, Globodera pallida. Newly hatched, infective second-stage juveniles (J2) were placed for 24, 48 and 72 h in solutions containing concentrations of the formulation from 30.080.0 μl l–1 with 20% (v/v) potato root leachate and sterile distilled water controls. The garlic extract, salicylaldehyde and surfactant treatments were assessed at concentrations proportional to their occurrence in the formulation. Hatching assays involved a series of experiments in which G. pallida cysts were incubated for 8 weeks in potato root leachate solution containing different concentrations of the test substances. A second set of experiments involved incubating cysts in different concentrations of the test substances for 2, 4 and 8 weeks prior to hatching in potato root leachate solution to determine how prior exposure to these substances influences hatching and in-egg viability. The formulation caused 100% mortality at 75.0 μl l–1 with an LC50 of 43.6 μl l–1 after 24 h exposure. Salicylaldehyde was the most toxic constituent of the formulation with an LC50 of 6.5 μl l–1 after 24 h, while the garlic extract achieved 50% J2 mortality at 983.0 μl l–1, demonstrating that the formulation and salicylaldehyde are more toxic to G. pallida in vitro than oxamyl but less toxic when compared with aldicarb. The surfactant showed no dose-dependent toxic effects on J2 when compared with the controls. Emergence of J2 from the cysts was significantly reduced by concentrations of the formulation above 688.0 μl l–1 and its equivalent concentration of salicylaldehyde, while concentrations of the formulation above 2752.0 μl l–1 and the corresponding salicylaldehyde concentrations resulted in complete irreversible hatch inhibition. Concentrations of the garlic extract below 137.6 μl l–1 caused 26% more J2 hatch in comparison to the potato root leachate solution. This study has shown that salicylaldehyde is more toxic to nematodes than the garlic extract, and is the first report of a hatch stimulatory effect of a garlic extract on G. pallida under in vitro conditions.
Humanitarian Narratives: Knowledge and the Politics of Mission and Empire
Authors: Ballantyne Tony 
Published: September 2011

AbstractThis essay offers a reassessment of the connections between missionary work and empire building. It focuses on the role of 'humanitarian narratives' in soliciting sympathy amongst British evangelicals and humanitarians for the 'suffering' of Maori during the 1820s and 1830s. Exploring the nature and influence of these narratives not only allows the historian to explore the connections between the empire and British metropolitan culture and politics in this period, but it also provides new insights into why New Zealand was finally incorporated into the British empire in 1840.
Women, Protestant Missions, and American Cultural Expansion, 1800 to 1938: A Historiographical Sketch
Authors: Reeves-Ellington Barbara 
Published: September 2011

AbstractOver the past forty years, historians' descriptions of American women missionaries have ranged from martyrs to cultural imperialists to social activists engaged with real people around the world to promote the welfare of women and children. New transnational approaches to the study of American missions abroad demonstrate that women missionaries were not a stock homogeneous group but a diverse group of individuals engaged in complex encounters with an equally diverse group of people in multiple settings. As scholars have highlighted the contributions of women missionaries to the projection of American Protestantism across the globe, they have increasingly recognized the importance of the cross-cultural connections that have given new meanings to the Protestant messages in local environments. This essay reviews the scholarship of the last forty years and indicates new avenues of research on American Protestant women in mission.
Validation of the web-based LUMINA questionnaire for recruiting large cohorts of migraineurs
Authors: van Oosterhout WPJ , Weller CM , Stam AH , Bakels F , Stijnen T , Ferrari MD , Terwindt GM 
Published: September 2011

Objective: To assess validity of a self-administered web-based migraine-questionnaire in diagnosing migraine aura for the use of epidemiological and genetic studies. Methods: Self-reported migraineurs enrolled via the LUMINA website and completed a web-based questionnaire on headache and aura symptoms, after fulfilling screening criteria. Diagnoses were calculated using an algorithm based on the International Classification of Headache Disorders (ICHD-2), and semi-structured telephone-interviews were performed for final diagnoses. Logistic regression generated a prediction rule for aura. Algorithm-based diagnoses and predicted diagnoses were subsequently compared to the interview-derived diagnoses. Results: In 1 year, we recruited 2397 migraineurs, of which 1067 were included in the validation. A seven-question subset provided higher sensitivity (86% vs. 45%), slightly lower specificity (75% vs. 95%), and similar positive predictive value (86% vs. 88%) in assessing aura when comparing with the ICHD-2-based algorithm. Conclusions: This questionnaire is accurate and reliable in diagnosing migraine aura among self-reported migraineurs and enables detection of more aura cases with low false-positive rate.
Application of different transient sorption methods to evaluate moisture diffusion coefficients of building materials on the hygroscopic range
Authors: Delgado JMPQ , Ramos NMM , de Freitas VP 
Published: September 2011

Typically, moisture transport coefficients are determined either by stationary measurements, as standard cup set-up and modified cup method, or by dynamic measurements, as the means of the type [Formula: see text] rule in the initial phase of adsorption processes, logarithmic procedure in the end stage of adsorption processes, the half-time method, the moment method, and the hybrid 1-term method. The aim of this work was to test the usability of the five dynamic calculation methods to define the moisture diffusivity in the hygroscopic range of different building materials. The results obtained were compared in order to evaluate the discrepancies between the different calculation techniques. Advantages in time saving using each experimental technique were compared with the feasibility of each process. Two commonly used building materials are considered in the study, gypsum board and gypsum plaster, for adsorptive measurement of the moisture diffusivity at three different temperatures in three different relative humidity ranges.
miRNA regulation of cytotoxic effects in mouse Sertoli cells exposed to nonylphenol
Authors: Choi Jin-Sung , Oh Jung-Hwa , Park Han-Jin , Choi Mi-Sun , Park Se-Myo , Kang Seung-Jun , Oh Moon-Ju , Kim Seung Jun , Hwang Seung Yong , Yoon Seokjoo 
Published: September 2011

Abstract

Background
It is known that some environmental chemicals affect the human endocrine system. The harmful effects of endocrine disrupting chemical (EDC) nonylphenol (NP) have been studied since the 1980s. It is known that NP adversely affects physiological functions by mimicking the natural hormone 17 beta-estradiol. In the present study, we analyzed the expression of miRNAs and their target genes in mouse Sertoli TM4 cells to better understand the regulatory roles of miRNAs on Sertoli cells after NP exposure.

Methods
Mouse TM4 Sertoli cells were treated with NP for 3 or 24 h, and global gene and miRNA expression were analyzed using Agilent mouse whole genome and mouse miRNA v13 arrays.

Results
We identified genes that were > 2-fold differentially expressed in NP-treated cells and control cells (P < 0.05) and analyzed their functions through Gene Ontology analysis. We also identified miRNAs that were differentially expressed in NP-treated and control cells. Of the 186 miRNAs the expression of which differed between NP-treated and control cells, 59 and 147 miRNAs exhibited 1.3-fold increased or decreased expression at 3 and 24 h, respectively. Network analysis of deregulated miRNAs suggested that Ppara may regulate the expression of certain miRNAs, including miR-378, miR-125a-3p miR-20a, miR-203, and miR-101a, after exposure to NP. Additionally, comprehensive analysis of predicted target genes for miRNAs showed that the expression of genes with roles in cell proliferation, the cell cycle, and cell death were regulated by miRNA in NP-treated TM4 cells. Levels of expression of the miRNAs miR-135a* and miR-199a-5p were validated by qRT-PCR. Finally, miR-135a* target gene analysis suggests that the generation of reactive oxygen species (ROS) following exposure to NP exposure may be mediated by miR-135a* through regulation of the Wnt/beta-catenin signaling pathway.

Conclusions
Collectively, these data help to determine NP's actions on mouse TM4 Sertoli cells and increase our understanding of the molecular mechanisms underlying the adverse effects of xenoestrogens on the reproductive system.
Evaluating the potential of a novel oral lesion exudate collection method coupled with mass spectrometry-based proteomics for oral cancer biomarker discovery
Authors: Kooren Joel A , Rhodus Nelson L , Tang Chuanning , Jagtap Pratik D , Horrigan Bryan J , Griffin Timothy J 
Published: September 2011

Abstract

Introduction
Early diagnosis of Oral Squamous Cell Carcinoma (OSCC) increases the survival rate of oral cancer. For early diagnosis, molecular biomarkers contained in samples collected non-invasively and directly from at-risk oral premalignant lesions (OPMLs) would be ideal.

Methods
In this pilot study we evaluated the potential of a novel method using commercial PerioPaper absorbent strips for non-invasive collection of oral lesion exudate material coupled with mass spectrometry-based proteomics for oral cancer biomarker discovery.

Results
Our evaluation focused on three core issues. First, using an "on-strip" processing method, we found that protein can be isolated from exudate samples in amounts compatible with large-scale mass spectrometry-based proteomic analysis. Second, we found that the OPML exudate proteome was distinct from that of whole saliva, while being similar to the OPML epithelial cell proteome, demonstrating the fidelity of our exudate collection method. Third, in a proof-of-principle study, we identified numerous, inflammation-associated proteins showing an expected increase in abundance in OPML exudates compared to healthy oral tissue exudates. These results demonstrate the feasibility of identifying differentially abundant proteins from exudate samples, which is essential for biomarker discovery studies.

Conclusions
Collectively, our findings demonstrate that our exudate collection method coupled with mass spectrometry-based proteomics has great potential for transforming OSCC biomarker discovery and clinical diagnostics assay development.
Ossification process involving the human thoracic ligamentum flavum: role of transcription factors
Authors: Uchida Kenzo , Yayama Takafumi , Cai Hong-Xin , Nakajima Hideaki , Sugita Daisuke , Guerrero Alexander Rodríguez , Kobayashi Shigeru , Yoshida Ai , Chen Ke-Bing , Baba Hisatoshi 
Published: September 2011

Abstract

Introduction

Ossification of the ligamentum flavum (OLF) of the spine is associated with serious neurologic compromise, but the pathomechanism of this process remains unclear. The objective of this study was to investigate the pathomechanism of the ossification process, including the roles of various transcriptional factors in the ossification of human thoracic ligamentum flavum.

Methods

Sections of the thoracic ligamentum flavum were obtained from 31 patients with OLF who underwent posterior thoracic decompression, and from six control patients free of OLF. Cultured ligamentum flavum cells (n = 6, each) were examined with real-time reverse transcription-polymerase chain reaction (RT-PCR) analysis for Sry-type high-mobility group box 9 (Sox9), runt-related transcription factor 2 (Runx2), muscle segment homeobox 2 (Msx2), Osterix, distal-less homeobox 5 (Dlx5), and AP-1. The harvested sections were examined with hematoxylin-eosin, the terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end-labeling (TUNEL) method, and immunohistochemistry for the transcriptional factors.

Results

Compared with the control, the OLF showed disorganization of the elastic fiber bundles and abundant hypertrophic chondrocytes in the ossification front. TUNEL-positive chondrocytes were found near the ossified plaques. The mRNA expression levels of Sox9, Runx2, Msx2, and AP-1 in cultured cells from the ligamentum flavum of OLF patients were significantly different from those of the control. OLF samples were strongly immunoreactive to Sox9, Runx2, and Msx2 at proliferating chondrocytes in the fibrocartilage area. Hypertrophic chondrocytes were positive for Runx2, Osterix, Dlx5, and AP-1.

Conclusions

The ossification process in OLF seems to involve chondrocyte differentiation under the unique expression of transcriptional factors. Accumulation of hypertrophic chondrocytes was evident around the calcified area at the ossification front, and we suggest that the differentiation of these cells seems to be concerned with the ossification process.
The effect of diabetes on mortality in critically ill patients: a systematic review and meta-analysis
Authors: Siegelaar Sarah E , Hickmann Maartje , Hoekstra Joost BL , Holleman Frits , DeVries J Hans 
Published: September 2011

Abstract
Introduction
Critically ill patients with diabetes are at increased risk for the development of complications, but the impact of diabetes on mortality is unclear. We conducted a systematic review and meta-analysis to determine the effect of diabetes on mortality in critically ill patients, making a distinction between different ICU types.

Methods
We performed an electronic search of MEDLINE and Embase for studies published from May 2005 to May 2010 that reported the mortality of adult ICU patients. Two reviewers independently screened the resultant 3,220 publications for information regarding ICU, in-hospital or 30-day mortality of patients with or without diabetes. The number of deaths among patients with or without diabetes and/or mortality risk associated with diabetes was extracted. When only crude survival data were provided, odds ratios (ORs) and standard errors were calculated. Data were synthesized using inverse variance with ORs as the effect measure. A random effects model was used because of anticipated heterogeneity.

Results
We included 141 studies comprising 12,489,574 patients, including 2,705,624 deaths (21.7%). Of these patients, at least 2,327,178 (18.6%) had diabetes. Overall, no association between the presence of diabetes and mortality risk was found. Analysis by ICU type revealed a significant disadvantage for patients with diabetes for all mortality definitions when admitted to the surgical ICU (ICU mortality: OR [95% confidence interval] 1.48 [1.04 to 2.11]; in-hospital mortality: 1.59 [1.28 to 1.97]; 30-day mortality: 1.62 [1.13 to 2.34]). In medical and mixed ICUs, no effect of diabetes on all outcomes was found. Sensitivity analysis showed that the disadvantage in the diabetic surgical population was attributable to cardiac surgery patients (1.77 [1.45 to 2.16], P < 0.00001) and not to general surgery patients (1.21 [0.96 to 1.53], P = 0.11).

Conclusions
Our meta-analysis shows that diabetes is not associated with increased mortality risk in any ICU population except cardiac surgery patients.
Airway response to acute mechanical stress in a human bronchial model of stretch
Authors: Faisy Christophe , Pinto Francisco M , Le Guen Morgan , Naline Emmanuel , Grassin Delyle Stanislas , Sage Edouard , Candenas Maria-Luz , Devillier Philippe 
Published: September 2011

Abstract
Introduction
Lung inflation may have deleterious effects on the alveoli during mechanical ventilation. However, the consequences of stretch during excessive lung inflation on basal tone and responsiveness of human bronchi are unknown. This study was undertaken to devise an experimental model of acute mechanical stretch in isolated human bronchi and to investigate its effect on airway tone and responsiveness.

Methods
Bronchi were removed from 48 thoracic surgery patients. After preparation and equilibration in an organ bath, bronchial rings were stretched for 5 min using a force (2.5 × basal tone) that corresponded to airway-inflation pressure > 30 cm H2O. The consequences of stretch were examined by using functional experiments, analysis of organ-bath fluid, and ribonucleic acid (RNA) isolation from tissue samples.

Results
Following removal of the applied force the airways immediately developed an increase in basal tone (P < 0.0001 vs. paired controls) that was sustained and it did so without significantly increasing responsiveness to acetylcholine. The spontaneous tone was abolished with a Rho-kinase inhibitor and epithelium removal, a leukotriene antagonist or nitric oxide synthase inhibitors reduced it, whereas indomethacin, sensory nerve inhibitors or antagonists for muscarinic, endothelin and histamine receptors had no effect. Stretch enhanced leukotriene-E4 production during the immediate spontaneous contraction of human bronchi (P < 0.05). Moreover, stretch up-regulated the early mRNA expression of genes involved in wingless-type mouse mammary tumor virus integration-site family (WNT)-signaling and Rho-kinase pathways.

Conclusions
Stretching human bronchi for only 5 min induces epithelial leukotriene release via nitric oxide synthase activation and provokes a myogenic response dependent on Rho-kinase and WNT-signaling pathways. From a clinical perspective, these findings highlight the response of human airway to acute mechanical stress during excessive pulmonary inflation.