bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cath6, a bHLH atonal family proneural gene, negatively regulates neuronal differentiation in the retina
Authors: Kubo Fumi , Nakagawa Shinichi 
Published: September 2010

AbstractBasic helix–loop–helix (bHLH) transcription factors play important roles in cell type specification and differentiation during the development of the nervous system. In this study, we identified a chicken homolog of Atonal 8/ath6 (Cath6) and examined its role in the developing retina. Unlike other Atonal‐family proneural genes that induce neuronal differentiation, Cath6 was expressed in stem cell‐like progenitor cells in the marginal region of the retina, and its overexpression inhibited neuronal differentiation. A Cath6 fused with a VP16 transactivation domain recapitulated the inhibitory effect of Cath6 on neuronal differentiation, indicating that Cath6 functions as a transcription activator. These results demonstrate that Cath6 constitutes a unique member of the Atonal‐family of genes in that it acts as a negative regulator of neuronal differentiation. Developmental Dynamics 239:2492–2500, 2010. © 2010 Wiley‐Liss, Inc.
Neuron‐specific expression of atp6v0c2 in zebrafish CNS
Authors: Chung Ah‐Young , Kim Myoung‐Jin , Kim Dohyun , Bang Sangsu , Hwang Sun Wook , Lim Chae Seung , Lee Sanggyu , Park Hae‐Chul , Huh Tae‐Lin 
Published: September 2010

AbstractVacuolar ATPase (V‐ATPase) is a multi‐subunit enzyme that plays an important role in the acidification of a variety of intracellular compartments. ATP6V0C is subunit c of the V0 domain that forms the proteolipid pore of the enzyme. In the present study, we investigated the neuron‐specific expression of atp6v0c2, a novel isoform of the V‐ATPase c‐subunit, during the development of the zebrafish CNS. Zebrafish atp6v0c2 was isolated from a genome‐wide analysis of the zebrafish mibta52b mutant designed to identify genes differentially regulated by Notch signaling. Whole‐mount in situ hybridization revealed that atp6v0c2 is expressed in a subset of CNS neurons beginning several hours after the emergence of post‐mitotic neurons. The ATP6V0C2 protein is co‐localized with the presynaptic vesicle marker, SV2, suggesting that it is involved in neurotransmitter storage and/or secretion in neurons. In addition, the loss‐of‐function experiment suggests that ATP6V0C2 is involved in the control of neuronal excitability. Developmental Dynamics 239:2501–2508, 2010. © 2010 Wiley‐Liss, Inc.
Specific isoforms of Drosophila shroom define spatial requirements for the induction of apical constriction
Authors: Bolinger Cory , Zasadil Lauren , Rizaldy Ryan , Hildebrand Jeffrey D. 
Published: September 2010

AbstractThe Drosophila Shroom protein, dShrmA, induces apical constriction when ectopically expressed in the embryonic ectoderm. An embryo expressing dShrmA in the dorsal ectoderm was stained to detect dShrm (green) and F‐actin (red). Inset shows a scanning electron micrograph of a similar staged embryo also expressing dShrmA in the dorsal ectoderm. From Bolinger et al., Developmental Dynamics 239:2078–2093, 2010.
Brain expression of Cre recombinase driven by pancreas‐specific promoters
Authors: Song Junghun , Xu Yuanzhong , Hu Xiaoxia , Choi Brian , Tong Qingchun 
Published: September 2010

AbstractCre‐loxP technology enables specific examination of the function and development of individual nuclei in the complex brain network. However, for most brain regions, the utilization of this technique has been hindered by the lack of mouse lines with Cre expression restricted to these regions. Here, we identified brain expressions of three transgenic Cre lines previously thought to be pancreas‐specific. Cre expression driven by the rat‐insulin promoter (Rip‐Cre) was found mainly in the arcuate nucleus, and to a lesser degree in other hypothalamic regions. Cre expression driven by the neurogenin 3 promoter (Ngn3‐Cre mice) was found in the ventromedial hypothalamus. Cre expression driven by the pancreas‐duodenum homeobox 1 promoter (Pdx1‐Cre) was found in several hypothalamic nuclei, the dorsal raphe and inferior olivary nuclei. Interestingly, Pdx1‐Cre mediated deletion of vesicular GABA transporter led to postnatal growth retardation while Ngn3‐Cre mediated deletion had no effects, suggesting a role for Pdx1‐Cre neurons, but not pancreas, in the regulation of postnatal growth. These results demonstrate the potential for these Cre lines to study the function and development of brain neurons. genesis 48:628–634, 2010. © 2010 Wiley‐Liss, Inc.
High‐bandwidth design of hard disk control system using H∞ control with feedback‐type uncertainty
Authors: Hirata Mitsuo , Hasegawa Yumi 
Published: September 2010

AbstractIn hard disk drives, it is important to enlarge the control bandwidth in order to shorten the track pitch for larger data capacity. However, it is difficult for the H∞ control method to increase the control bandwidth if the mechanical resonance modes have uncertainty. This is because the robustness of the H∞ control method is assured by the small‐gain theorem for additive or multiplicative perturbation and the control bandwidth is limited by the uncertainty. In this study, we propose an H∞ control method for high‐bandwidth design by introducing a new uncertainty model with a feedforward and a feedback path in order to reduce the conservatism of robust design. The effectiveness is shown by numerical simulations. © 2010 Wiley Periodicals, Inc. Electr Eng Jpn, 173(4): 54–62, 2010; Published online in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/eej.21025
Reactions of [Re(CO)3]+ with Histidylhistidine and Modified Histidines (Eur. J. Inorg. Chem. 25/2010)
Authors: Herrick Richard S. , Ziegler Christopher J. , Gambella Americo 
Published: September 2010

AbstractThe cover picture shows the crystal structures of two complexes that were prepared from aqueous reactions of histidine‐based ligands with Re(CO)3(H2O)3+ and serve as models of 99mTc(CO)3+/His‐tag protein complexes. The complexes, similar to those found in certain enzymes, show unexpected peptide carboxamido‐N donor bonds that underscore the stability of the labeled His‐tag proteins as discussed in the Short Communication by R. S. Herrick, C. J. Ziegler et al. on p. 3905 ff. Artwork: Christopher J. Ziegler.
A General and Highly Efficient Method for the Construction of Aryl‐Substituted N‐Heteroarenes
Authors: Liu Chun , Han Na , Song Xiaoxiao , Qiu Jieshan 
Published: September 2010

AbstractA general, simple and highly efficient method has been developed for the Pd(OAc)2‐catalyzed ligand‐free and aerobic Suzuki reaction of N‐heteroaryl halides, which is strongly dependent on the molecular structure of solvent.
Facilitated Transfer of Alkali and Alkaline‐Earth Metal Ions by a Calix[4]arene Derivative Across Water/1,2‐Dichloroethane Microinterface: Amperometric Detection of Ca2+
Authors: Bingol Haluk , Kaykal Ferhat , Akgemci Emine G. , Sirit Abdulkadir 
Published: September 2010

AbstractThe host–guest complexation reactions between 5,11,17,23‐tetra‐tert‐butyl‐25,27‐diethoxycarbonylmethoxy‐26,28‐dimethoxy calix[4]arene (BDDC4) and alkali and alkaline‐earth metal ions were investigated by facilitated ion transfer processes across water/1,2‐dichloroethane microinterface by using steady‐state cyclic voltammetry and differential pulse voltammetry. The obtained facilitated transfers for Li+, Na+, K+, Rb+ and Ca2+ were evaluated under the different experimental conditions, at the excess concentrations of metal ions with respect to BDDC4 and vice versa. The association constants having 1 : 1 stoichiometry for Li+, Na+, K+ and Rb+ in 1,2‐DCE were determined. Also, we demonstrated that BDDC4 can play an important role for the development of highly selective chemical sensor for Ca2+ among alkaline‐metal ions in the concentration range of 0.1–1.0 mM in aqueous solution.
Determination of Lead in Ambient Aerosol Samples by Anodic Stripping Voltammetry on a Bismuth Film Electrode
Authors: Marín Mª Rosario Palomo , Gil Eduardo Pinilla , Blázquez Lorenzo Calvo 
Published: September 2010

AbstractCd and Pb determination in ambient aerosol samples based on SWASV at a Bi film glassy carbon electrode is described. Extremely acid solutions resulting from sample digestion were directly assayed, with sufficient detection limits for testing the fulfilment of air quality regulations. Accuracy was tested on NIST Standard Reference Materials and real aerosol samples, with good recoveries for Pb comparable to ICP‐MS results. Cd was not detected, probably due to the combined effects of low Cd and high interfering Zn and Cu contents. The proposed method provides an attractive alternative to ICP‐MS with potential applicability for decentralized analysis.
Optical features of the fluorophore azotobactin: Applications for iron sensing in biological fluids
Authors: Sharma Manisha , Gohil Nivedita Karmakar 
Published: September 2010

AbstractSiderophores are bio‐organic ligands secreted by microbes to chelate and assimilate iron to meet their metabolic requirements. Siderophores and their analogs have tremendous therapeutic and analytical potential including the use as Fe (III) biosensors; however, only few practical applications have been realized. The aim of this study was the optical and biophysical characterization of the siderophore azotobactin (Az) secreted by the nitrogen‐fixing bacteria Azotobacter vinelandii. The peptide exhibited fluorescence in the visible range. Quantum yield and lifetime in excited state were measured to ascertain the sensitivity of the molecule as a fluorescent marker in biochemical assays. Its high affinity toward iron in the ferric state was demonstrated through fluorescence emission quenching studies. The accuracy of azotobactin as biosensing tool was determined by analyzing the levels of iron in biological fluids, particularly in human serum. Furthermore, it was demonstrated that it can be encapsulated in sol–gel matrices without significant loss of its fluorescence signal, thus enabling it suitable for adaptation to optical biosensor for Fe (III).
Dementia services in Canada
Authors: Rockwood Kenneth , Keren Ron 
Published: September 2010

AbstractCanadians with dementia have access to Medicare, a universal, single payer healthcare program. Implementation of Medicare is through the provinces and territories, giving variation in the level of care available. At present, there is no national strategy for dementia, although a recent report from the Alzheimer Society of Canada is expected to catalyze one. Most dementia care is provided by primary care practitioners, with three specialties (geriatric psychiatry, geriatric medicine and neurology) providing consultant expertise. Primary care reforms are aimed at developing a more coordinated approach to the complex needs of people with dementia, and have especially emphasized education of providers. Any national strategy is expected to underscore prevention and research, the latter building on Canada's strong contribution to this international undertaking. Copyright © 2010 John Wiley & Sons, Ltd.
Malnutrition and quality of life in patients treated for oral or oropharyngeal cancer
Authors: Jager‐Wittenaar Harriët , Dijkstra Pieter U. , Vissink Arjan , van der Laan Bernard F. A. M. , van Oort Rob P. , Roodenburg Jan L. N. 
Published: September 2010

AbstractBackground.This study assessed whether malnourished patients score lower on quality of life after treatment for oral/oropharyngeal cancer.Methods.Malnutrition (weight loss ≥10% in 6 months/≥5% in 1 month) and quality of life (European Organization for Research and Treatment of Cancer Quality of Life Questionnaire Core 30 questionnaire) were assessed cross‐sectionally in patients treated for oral/oropharyngeal cancer. The interval after treatment varied from 1 day to 3 years. The relationship between malnutrition and quality of life was analyzed univariately (Mann–Whitney U test) and multivariately (linear regression analyses). Statistical significance was set at p < .05.Results.Prevalence of posttreatment malnutrition was 16% (18/115, 95% confidence interval [CI]: 9% to 23%). Analyzed univariately, malnourished patients scored significantly worse on physical functioning (p = .007) and fatigue (p = .034) than well‐nourished patients. Multivariate analysis revealed that malnutrition was significantly related to physical functioning (p = .015).Conclusions.Malnourished patients treated for oral/oropharyngeal cancer score lower on quality of life scales related to physical fitness. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Cysteinyl leukotriene receptor expression pattern affects migration of breast cancer cells and survival of breast cancer patients
Authors: Magnusson Cecilia , Liu Jian , Ehrnström Roy , Manjer Jonas , Jirström Karin , Andersson Tommy , Sjölander Anita 
Published: September 2010

AbstractThe fact that breast cancer patients with local or distal dissemination exhibit decreased survival, promotes a search for novel mechanisms to suppress such tumor progression. Here, we have determined the expression of proinflammatory cysteinyl leukotriene receptors (CysLTRs) in breast tumor tissue and their signaling effect on breast cancer cell functions related to tumor progression. Patients with breast tumors characterized by high CysLT1R and low CysLT2R expression levels exhibited increased risk of cancer‐induced death in univariate analysis for both the total patient group (hazard ratio [HR] = 2.88, 95% confidence interval [CI] = 1.11–7.41), as well as patients with large (>20 mm) tumors (HR = 5.08, 95% CI = 1.39–18.5). Multivariate analysis revealed that patients with large tumors exhibiting high CysLT1R and low CysLT2R expression levels had a significantly reduced survival, also when adjusted for established prognostic parameters (HR = 7.51, 95% CI = 1.83–30.8). In patients with large (>20 mm) tumors, elevated CysLT2R expression predicted an improved 5‐year survival (log‐rank test p = 0.04). Surprisingly, for longer time periods, this prognostic value was lost. This disappearance coincided with the termination of hormonal treatment. Tamoxifen preserved and even induced transcription of CysLT2R, but not CysLT1R, in estrogene receptor‐positive MCF‐7 breast cancer cells. This elevated CysLT2R expression decreased, even below the level of untreated cells, when tamoxifen was withdrawn. CysLT2R signaling reduced MCF‐7 cell migration, but had no effect on either proliferation or apoptosis. Our data indicate that low CysLT1R together with high CysLT2R expression levels might be useful parameters in prognostication and treatment stratification of breast cancer patients.
Electrospun cross‐linked gelatin fibers with controlled diameter: The effect of matrix stiffness on proliferative and biosynthetic activity of chondrocytes cultured in vitro
Authors: Skotak Maciej , Noriega Sandra , Larsen Gustavo , Subramanian Anuradha 
Published: September 2010

AbstractNanofibrous scaffolds were prepared from gelatin solutions and were further cross‐linked with glutaraldehyde (GA). The fiber diameter was varied from 100 to 1000 nm by controlling the applied voltage (4–15 kV) and the concentration of the gelatin solution (4–15%). The tensile moduli and the tensile strength of the noncross‐linked scaffolds varied from 20 to 120 MPa and 0.5 to 3.5 MPa, respectively. Cross‐linking with GA led to an increase in both the tensile modulus and strength and correlated with cross‐linker concentration. Gelatin‐based matrices were characterized by Fourier transform infrared spectroscopy and differential scanning calorimetry. High cellular viabilities and rounded morphology of chondrocytes was observed at the end of 7 days in culture with added matrix deposition and flattening of cells at 15 days. Matrix stiffness was noted to impact cell densities and the expression of chondrocytic markers, especially aggrecan. The ratios of collagen‐II (C‐II) to collagen‐I (C‐I) of 0.62 and 1.33 were noted on gelatin nanofibrous scaffolds cross‐linked with 0.1% GA at the end of 7 and 15 days in culture, respectively. C‐II/C‐I ratios of 1.30 and 2.58 were noted on scaffolds cross‐linked with 1.0% GA at the end of 7 and 15 days in culture, respectively. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Differential mechanisms for the inhibition of human cytochrome P450 1A2 by apigenin and genistein
Authors: Shimada Hideaki , Eto Masashi , Ohtaguro Misa , Ohtsubo Michihiro , Mizukami Yosuke , Ide Tsuyoshi , Imamura Yorishige 
Published: September 2010

AbstractThe inhibitory effects of flavonoids on the human cytochrome P450 1A2 (CYP1A2) were examined. Among flavonoids tested, galangin, kaempferol, chrysin, and apigenin were potent inhibitors. Although apigenin belonging to flavones and genistein belonging to isoflavones are similar in the chemical structures, the inhibitory potencies for CYP1A2 were distinguished markedly between these two flavonoids. In computer‐docking simulation, apigenin interacted with the same mode of cocrystallized α‐naphthoflavone in the active site of CYP1A2, and then the B ring of apigenin was placed close to the heme iron of the enzyme with a single orientation. In contrast, the docked genistein conformation showed two different binding modes, and the A ring of genistein was oriented to the heme iron of CYP1A2. Furthermore, the binding free energy of apigenin was lower than that of genistein. These results demonstrate a possible mechanism that causes the differential inhibitory potencies of apigenin and genistein for CYP1A2. © 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 24:230–234, 2010; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20328
How to transition from assessing performance to enhancing performance with balanced scorecard goal action plans
Authors: Albright Thomas L. , Burgess Christopher M. , Hibbets Aleecia R. , Roberts Michael L. 
Published: September 2010

AbstractThe balanced scorecard (BSC) has achieved widespread acceptance as a strategic performance measurement tool. This article builds on the BSC process by showing how goal action plans can be used to help organizations translate strategic goals into actionable employee behavior to improve bottom‐line performance. © 2010 Wiley Periodicals, Inc.
A Raman spectroscopic study of copiapites Fe2+Fe3+(SO4)6(OH)2 · 20H2O: environmental implications
Authors: Frost Ray L. 
Published: September 2010

AbstractRaman spectroscopy has been used to study selected mineral samples of the copiapite group. Copiapite (Fe2+Fe3+(SO4)6(OH)2 · 20H2O) is a secondary mineral formed through the oxidation of pyrite. Minerals of the copiapite group have the general formula AFe4(SO4)6(OH)2 · 20H2O, where A has a + 2 charge and can be either magnesium, iron, copper, calcium and/or zinc. The formula can also be B2/3Fe4(SO4)6(OH)2 · 20H2O, where B has a + 3 charge and may be either aluminium or iron. For each mineral, two Raman bands are observed at around 992 and 1029 cm−1, assigned to the (SO4)2−ν1 symmetric stretching mode. The observation of two bands provides evidence for the existence of two non‐equivalent sulfate anions in the mineral structure. Three Raman bands at 1112, 1142 and 1161 cm−1 are observed in the Raman spectrum of copiapites, indicating a reduction of symmetry of the sulfate anion in the copiapite structure. This reduction in symmetry is supported by multiple bands in the ν2 and ν4(SO4)2− spectral regions. Copyright © 2010 John Wiley & Sons, Ltd.
Ultrasound‐guided needle aspiration: Impact of immediate cytologic review
Authors: Moberly Aaron C. , Vural Emre , Nahas Bari , Bergeson Tonya R. , Kokoska Mimi S. 
Published: September 2010

AbstractObjectives/Hypothesis:To compare the diagnostic adequacy of ultrasound‐guided fine‐needle aspiration biopsy (USFNA) of the head and neck with immediate on‐site microscopic evaluation and feedback by a cytopathologist (IMMEDIATE) versus delayed examination without immediate feedback (DELAYED).Study Design:Retrospective chart review of office‐based USFNA performed in both IMMEDIATE and DELAYED settings by the same otolaryngologist.Methods:A total of 199 USFNA procedures in the head and neck were performed by one practitioner. Of these procedures, 137 biopsies were performed in the DELAYED setting, whereas 62 biopsies were performed in the IMMEDIATE setting. Cytopathology reports were reviewed for diagnostic conclusions as well as adequacy of biopsies. The results from both settings were compared using Pearson χ2 test.Results:The ability to obtain a diagnosis from USFNA was 73.0% in the DELAYED group as compared with 90.3% in the IMMEDIATE group. The rate of adequate cells on biopsy was 89.1% in the DELAYED group as compared with 96.8% in the IMMEDIATE group. There was a statistically significant difference between observed and expected diagnostic rates (χ2[1] = 7.568, P = .006) and a trend toward a significant difference between the observed and expected adequacy rates (χ2[1] = 3.259, P = .071) when IMMEDIATE evaluation by a cytopathologist was performed.Conclusions:An immediate microscopic evaluation of USFNA specimens to confirm adequacy of sampling is strongly recommended. Diagnostic conclusions were increased when an evaluation of the specimen was performed at the time of biopsy. The specimen adequacy rate tended to increase with an immediate cytopathologic assessment. Laryngoscope, 2010
Balloon catheter technology in rhinology: Reviewing the Evidence
Authors: Batra Pete S. , Ryan Matthew W. , Sindwani Raj , Marple Bradley F. 
Published: September 2010

AbstractBalloon catheter technology (BCT) for management of paranasal sinus inflammatory disease was introduced to otolaryngology in 2005. Since its introduction, BCT has been a subject of considerable controversy with proponents for and against adoption of the technology. Balloon procedures have been promoted as a less invasive alternative to endoscopic sinus surgery that results in reduced pain and quicker recovery. The technology and its promotion have generated significant press coverage and interest by the lay public looking for new solutions for sinonasal problems. Over time, alternate balloon devices have been advocated for operating room and office‐based sinus ostia dilatation. This contemporary review will evaluate the existing evidence on the available balloon devices. The frank strengths and weaknesses of the peer‐reviewed literature will be highlighted. The potential complications unique to balloon catheters and radiation exposure from fluoroscopy will also be discussed. Laryngoscope, 2011