bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reconstruction of artificial nuclei with nuclear import activity in living mouse oocytes
Authors: Yonezawa Nao , Shindo Tomoko , Oda Haruka , Kimura Hiroshi , Hiraoka Yasushi , Haraguchi Tokuko , Yamagata Kazuo 
Published: May 2024

AbstractThe cell nucleus is a dynamic structure repeating disassembly and reformation during mitosis. Reformation of the nucleus is essential for cell proliferation, and therefore, the factors required for nuclear reformation are fundamental for eukaryotes. Although various factors have been identified inin vitrosystems using frog egg extracts andin vivoimaging of somatic cells, little is known about the factors required for the formation of functional nuclear structures in living mouse eggs. To identify such factors, we used a reconstruction approach to construct an artificial nucleus around DNA in mouse eggs. T4 phage DNA (166 kbp) was microinjected into living mouse oocytes. Amounts of DNA injected and injection timing were examined to determine the conditions appropriate for the formation of functional nuclei. Microinjection of 100-500 ng/µl DNA during metaphase through telophase of the second meiosis, but not the subsequent interphase, was important for the formation of artificial nucleus. This T4 DNA-derived artificial nucleus had the structure of nuclear lamina and nuclear pore complexes, and nuclear transport activity, similar to natural nuclei. These results suggest that exogenous DNA can form a functional nucleus in mouse oocytes, regardless of the sequence or the source of DNA.
Exploring the genome-wide expression level of the bacterial strain belonging toBacillus safensis(MM19) againstPhomopsis viticola
Authors: Silme Ragıp Soner , Baysal Ömür , Can Ahmet , Kürüm Yiğit , Korkut Ahmet , Kırboga Kevser Kübra , Çetinkaya Agit 
Published: May 2024

AbstractIntroductionRhizobacteria has the suppression ability to compete with pathogenic microorganisms and help for plant immunity and defense mechanism. Their growth and survival in rhizosphere ensure biological balance in favor of the host plant.MethodsIn our study, with agar diffusion assays, we found a rhizobacterium species belonging toBacillus safensis, which can significantly suppressPhomopsis viticola. To elucidate the antagonistic mechanism, genome-wide gene expression profiling ofB. safensis(strain MM19) was performed in the presence and absence ofP. viticola. We used RNA-seq analysis to obtain a comprehensive overview of the responsiveB. safensiswhole gene expression classified according to biological and metabolic process toP. viticolaconcomitant growth in liquid culture.ResultsThe differential gene expression profiles ofB. safensis(MM19) revealed significantly increased expression of prominent genes related to thiamine biosynthesis involving various metabolites and enzymes that play role in the suppression of mycelium growth and pathogen inhibition. Correspondingly, the expression of three major genes (HOG1, FUS3, SGI) involving in the virulence ofP. viticolawas followed using qPCR analysis. HOG1 was the highest expressed gene in the pathogen when it co-cultivated with MM19. Based on these findings, we performed molecular docking and dynamics analysis to explore the interaction between HOG1 and thiamine, besides expression of network analysis constructed using Cytoscape.DiscussionThe results proved that the functional genomic data related to thiamine biosynthesis and corresponding pathways ensure a priming role in the antagonistic behavior ofB. safensis(MM19) againstP. viticolaas a supporter for plant immunity.
In vivo reprogramming ofCaenorhabditis elegansleads to heterogeneous effects on lifespan
Authors: Kamaludeen Nibrasul , Mauge Yann , Mouridi Sonia El , Picó Sara , Vílchez-Acosta Alba , de Sousa João Agostinho , Pierron Marie , Praz Viviane , von Meyenn Ferdinand , Frøkjær-Jensen Christian , Ocampo Alejandro 
Published: May 2024

SUMMARYIn the last decade, cellular reprogramming of fully differentiated cells to pluripotent stem cells has become of great interest. Importantly, cellular reprogramming by expression of Oct4, Sox2, Klf4, and cMyc (OSKM) can ameliorate age-associated phenotypes in multiple tissues and extend lifespan in progeroid and aged wild-type mice. Surprisingly, the effects of in vivo reprogramming have not been deeply investigated in any other model organisms. Here, for the first time, we induce in vivo reprogramming inC. elegansusing a heat-inducible system at multiple developmental and adult stages. Similar to mice, expression of the reprogramming factors leads to premature death with different levels of toxicity at distinct developmental stages and aging. In vivo reprogramming inC. elegansmight represent a valuable tool to improve our understanding of development and in vivo reprogramming.
Does age protect against loss of tonotopy after acute deafness in adulthood?
Authors: Rosskothen-Kuhl Nicole , Green Sarah , Jakob Till F. 
Published: May 2024

AbstractThe mammalian auditory system develops a topographical organization of sound frequencies along its pathways, also called tonotopy, as a result of early auditory input. In contrast, sensory deprivation during early development results in no or only rudimentary tonotopic organization. This study addresses two questions: 1) How robust is the tonotopy when hearing fails in adulthood? 2) What role does age play at time of deafness? To address these questions, we deafened young and old adult rats with normal hearing. One month after deafness, both groups were unilaterally supplied with cochlear implants and electrically stimulated for two hours. The central auditory neurons, which were activated as a result of the local electrical intracochlear stimulation, were visualized using Fos staining. While the auditory system of young rats lost the tonotopic organization throughout the brainstem, the auditory system of the older rats mainly sustained its tonotopy. It can be proposed that neuronal plasticity prevails in the central auditory system of young adult rats, while neuronal stability prevails in the brains of aging rats. Consequently, age may be an important factor in protecting a hearing-experienced adult auditory system from a rapid loss of tonotopy when suffering from acute hearing loss. Furthermore, the study provides compelling evidence that acute deafness in young adult patients should be diagnosed as early as possible to prevent maladaptation of the central auditory system and thus achieve the optimal hearing outcome with a hearing prosthesis.
Two routes to value-based decisions in Parkinson’s disease: differentiating incremental reinforcement learning from episodic memory
Authors: Montaser-Kouhsari Leila , Nicholas Jonathan , Gerraty Raphael T. , Shohamy Daphna 
Published: May 2024

AbstractPatients with Parkinson’s disease are impaired at incremental reward-based learning. It is typically assumed that this impairment reflects a loss of striatal dopamine. However, many open questions remain about the nature of reward-based learning deficits in Parkinson’s. Recent studies have found that a combination of different cognitive and computational strategies contribute even to simple reward-based learning tasks, suggesting a possible role for episodic memory. These findings raise critical questions about how incremental learning and episodic memory interact to support learning from past experience and what their relative contributions are to impaired decision-making in Parkinson’s disease. Here we addressed these questions by asking patients with Parkinson’s disease (n=26) both on and off their dopamine replacement medication and age- and education-matched healthy controls (n=26) to complete a task designed to isolate the contributions of incremental learning and episodic memory to reward-based learning and decision-making. We found that Parkinson’s patients performed as well as healthy controls when using episodic memory, but were impaired at incremental reward-based learning. Dopamine replacement medication remediated this deficit while enhancing subsequent episodic memory for the value of motivationally relevant stimuli. These results demonstrate that Parkinson’s patients are impaired at learning about reward from trial-and-error when episodic memory is properly controlled for, and that learning based on the value of single experiences remains intact in patients with Parkinson’s disease.
Anchorage-independent cell proliferation promoted by fascin’s F-actin bundling
Authors: Isogai Tadamoto , Murali Vasanth Siruvallur , Zhou Felix , Wang Xinxin , Rajendran Divya , Perez-Castro Lizbeth , Venkateswaran Niranjan , Conacci-Sorrell Maralice , Danuser Gaudenz 
Published: May 2024

SummaryThe actin filament (F-actin) bundling protein fascin-1 is highly enriched in many metastatic cancers. Fascin’s contribution to metastasis have been ascribed to its enhancement of cell migration and invasion. However, mouse genetic studies clearly point to functions also in tumorigenesis, yet without mechanistic underpinnings. Here, we show that fascin expression promotes the formation of a non-canonical signaling complex that enables anchorage-independent proliferation. This complex shares similarities to focal adhesions and we refer to them as pseudo-adhesion signaling scaffolds (PASS). PASS are enriched with tyrosine phosphorylated proteins and require fascin’s F-actin-bundling activity for its assembly. PASS serve as hubs for the Rac1/PAK/JNK proliferation signaling axis, driven by PASS-associated Rac-specific GEFs. Experimental disruption of either fascin or RacGEF function abrogates sustained proliferation of aggressive cancersin vitroandin vivo. These results add a new molecular element to the growing arsenal of metabolic and oncogenic signaling programs regulated by the cytoskeleton architecture.
Supply chain collaboration, agility and firm performance: a case of manufacturing SMEs in India
Authors: Mahesh Prabhu H. , Srivastava Amit Kumar , Mukul Muthappa K.C. 
Published: May 2024

PurposeThe dynamic business environment and intense competition have mandated agility in operations for manufacturing firms. Effective inter-organizational collaboration can make operations more agile. This paper develops an interpretive model to explore the effect of supply chain collaboration (SCC) on supply chain agility (SCA) and, subsequently, on business performance.Design/methodology/approachA hierarchical model that illustrates the relationship between SCC, SCA and firm performance components is developed using total interpretative structural modeling (TISM). Also, statistical validation of the model has been performed.FindingsThe results indicate that the vision and alertness of the firm on the strategic front promote collaboration between supply chain partners. This creates operational agility, helping the firm to absorb fluctuations in demand, thereby enhancing business performance.Research limitations/implicationsThe opinion of most respondents was considered to develop the TISM framework over the fuzzy one, which necessitates a significantly more extensive data set. However, multiple discussions with participants can eliminate the prejudice of the majority approach. Also, the paper's development and validation were restricted to Indian manufacturing small and medium-sized enterprises (SMEs). The model can potentially be evaluated in large organizations to provide further insights.Originality/valueThe study blends the factors of SCC and SCA in a novel way to explain their combined impact on business performance. The TISM model addresses the “why” of theory development in addition to the “what” and “how” of it. Using triangulation in combination with the interpretative tool, this study additionally offers methodological value.
DRR pioneers: interview with Ben Wisner
Authors: Wisner Ben 
Published: May 2024

PurposeThe transcript provides an overview of the development of the field and changing paradigms in this regard.Design/methodology/approachThe transcript was developed in the context of a United Nations Office for Disaster Risk Reduction (UNDRR) project on the history of disaster risk reduction (DRR).FindingsThe transcript traces the initial discussions of how the At Risk book was conceived and presents new dimensions and challenges within the field.Originality/valueThe interview highlights the importance of the need to document the transitions, developments and paradigm changes in the field over time.
Institutional framework, macroeconomic instability and financial markets: perspective from emerging economies
Authors: Abaidoo Rexford , Agyapong Elvis Kwame 
Published: May 2024

Purpose The study evaluates the role of institutional framework and macroeconomic instability on financial market development among emerging economies.Design/methodology/approach The study uses panel data compiled from 32 countries from the sub-region of Sub-Sahara Africa (SSA), covering the period starting from 1996 to 2019. Empirical analyses were carried out using the two-step system generalized method of moments (TS-GMM) statistical framework.Findings Reviewed results suggest that institutional quality, effective governance and corruption control have a significant positive impact on financial market development among economies in the sub-region. Further empirical estimates show that macroeconomic risk and macroeconomic uncertainty have significant adverse effects on financial market development. Additionally, reported empirical estimates suggest that an improved institutional framework has the potential to lessen the adverse effect of macroeconomic instability on financial market development among economies in the sub-region.Originality/value The uniqueness of this empirical inquiry compared to related studies in the present literature stems from the fact that studies employing similar empirical approaches on the subject matter for economies in the sub-region are rare. Additionally, the analysis pursued in this study employs critical variables whose impact on financial market performance in the sub-region has not been examined per our review. These variables include indexes such as macroeconomic risk and institutional quality, which are unique to this study based on their construction; these indexes are generated using a principal component analysis procedure with different underlying variables compared to what may be found in the literature.
Supply chain concentration and financial performance: the moderating roles of marketing and operational capabilities
Authors: Wei Shaobo , Deng Chengnan , Liu Hua , Chen Xiayu 
Published: May 2024

PurposeBased on resource dependence theory (RDT) and transaction cost theory (TCT), we aim to investigate the relationship between supply chain concentration and firm performance. Based on the resource-based perspective, we further investigate the moderating effect of marketing and operational capabilities on the relationship between supply chain concentration and firm performance.Design/methodology/approachBased on data from 2,082 firms with 8,371 observations from 2008 to 2020 in China, we use stochastic frontier analysis to calculate marketing capability and operational capability and use multinational regressions to test our research model.FindingsWe find a U-shaped relationship between supplier concentration and firm performance; there is also a U-shaped relationship between customer concentration and firm performance. In addition, the relationship between supplier concentration and firm financial performance is strengthened by the firm’s marketing capability, and the relationship between customer concentration and firm financial performance is weakened by the firm’s operational capability.Originality/valueDrawing from RDT and TCT, this study extends the research on the impact of supply chain concentration on firm performance. The study finds that supply chain concentration and firm performance have a nonlinear relationship, and it is further moderated by marketing capability and operational capability, providing insights for managers.
“All employees are equal … but some are more equal than others”. Role identity and nonfamily member discrimination in family SMEs
Authors: Ferrari Filippo 
Published: May 2024

PurposeThis paper aims to investigate if, under which conditions, and with which consequences, nonfamily members have the perception of being discriminated against as a consequence of nepotism and adverse selection practices. This research also aims to investigate whether the carried-out role influences the perception of being discriminated against among nonfamily member employees.Design/methodology/approachA quantitative approach was carried out by adopting a structural equation model (SEM) analysis. The survey investigated a sample of Italian family SMEs (participating companies N = 186, total questionnaires collected N = 838).FindingsDrawing on the multiple identities theory, findings show that role salience (RS) effectively contributes to reducing the unwanted effects of perceived discrimination (PD) among nonfamily member employees. In doing so, this study deepens the knowledge of nonfamily member employment conditions and their consequences on strategic outcomes such as organizational commitment (OC), organizational justice (OJ) and intention to quit (ITQ).Research limitations/implicationsBy adopting a self-categorization approach, this study also advances current theoretical literature, as this methodological lens could help scholars further understand diversity in family business.Practical implicationsThis study suggests it would be advisable to implement human resource management practices based on job rotation to promote cohesion and reduce perceived distances.Social implicationsSMEs are the most widespread type of firm in the world; as a consequence, avoiding PD among nonfamily member employees has general ethical relevance.Originality/valueThis study expands current literature by showing that RS plays an important role in determining levels of PD. This study also advances current literature by focusing on the impact of multiple identities on fairness and commitment at individual and group levels of analysis of family businesses.
The Effects of Mandatory ESG Disclosure Around the World
Authors: KRUEGER PHILIPP , SAUTNER ZACHARIAS , TANG DRAGON YONGJUN , ZHONG RUI 
Published: May 2024

ABSTRACTWe compile a novel data set on mandatory environmental, social, and governance (ESG) disclosure around the world to analyze the stock liquidity effects of such disclosure mandates. We document a positive effect of ESG disclosure mandates on firm‐level stock liquidity. The effects are strongest if the disclosure requirements are implemented by government institutions, not on a comply‐or‐explain basis, and coupled with strong enforcement by informal institutions. Firms with weaker information environments benefit more from ESG disclosure mandates. Our results support the view that ESG disclosure regulation improves the information environment and has beneficial capital market effects.
Bangladesh and New Development Bank (NDB): Accession and after, money and more
Authors: Chin Gregory T. , Kamal Rifat D. 
Published: May 2024

AbstractThis article examines Bangladesh's new membership in the BRICS‐led New Development Bank (NDB), as the Bangladesh country study for the Special Section on ‘The Evolution of New Development Bank: A Decade Plus in the Making’, in Global Policy journal. In September 2021, Bangladesh became the first country outside of the original BRICS founding members to join NDB. The analysis details how Bangladesh went about joining NDB, especially the geopolitics and diplomacy between Bangladesh and the key NDB founding members (India, China, Russia) which supported Bangladesh's entry; why Bangladesh joined NDB, the main motivations or interests; and how NDB membership is turning out for Bangladesh after acceding to the Bank, what are Bangladesh and the Bank actually doing together, are they realizing the main opportunities as intended, or are outcomes not as intended. Throughout the analysis, we assess how Bangladesh's membership in NDB compares to its multi‐decade memberships in the World Bank, Asian Development Bank (ADB), Islamic Development, and its newer relations with the other new China‐supported multilateral bank, Asian Infrastructure Investment Bank (AIIB). This study combines primary data gathered from field research, primary desk research, and cross‐verification with the related data, and secondary sources.
Initial screening for neuronal autoantibodies and their putative impact on survival in patients with small‐cell lung cancer
Authors: Mikkelsen Anne With , Nilsson Anna Christine , Tenstad Helene Broch , Lillevang Soeren Thue , Asgari Nasrin 
Published: May 2024

AbstractIntroductionSmall‐cell lung cancer (SCLC) may be associated with neuronal autoantibodies and paraneoplastic neurological syndromes. It has been suggested that neuronal autoantibodies, especially antineuronal nuclear antibody type 1 (Hu) autoantibodies, are associated with longer survival of patients with SCLC. The objective of this study was to determine the frequency and distribution of neuronal autoantibodies at the time of diagnosis of SCLC patients and assess survival rates in relation to autoimmunity.MethodsIn this retrospective study, serum from 40 patients with biopsy‐proven SCLC at the time of diagnosis was studied prior to treatment. The sera originated from a cancer registry at the Oncology Department, Vejle Hospital from 2007 to 2010. The sera were analyzed blindly to clinical status for the presence of neuronal autoantibodies. Medical records were reviewed for neurological symptoms.ResultsNeuronal autoantibodies were detected in 22/40 (55%) of the SCLC patients. A broad range of neurological symptoms was recorded in 28/40 (70%) patients, of which 14/28 (50%) were positive for neuronal autoantibodies. The most frequently detected autoantibodies were Hu (7/40, 17.5%) followed by GAD65 (6/22, 15.0%). Striational and P/Q‐ or N‐type voltage‐gated calcium channel antibodies were less common, with each found in five patients (12.5%). Eight patients (20%) had coexisting autoantibodies. Autoantibody‐positivity was not associated with survival.ConclusionNeuronal autoantibodies were at time of diagnosis found in approximately half of the treatment‐naïve SCLC patients. Neither autoantibody positivity at diagnosis nor neurological manifestations correlated with survival and their clinical importance requires further studies in larger, prospective cohorts.
Skull asymmetry in various sheep breeds: Directional asymmetry and fluctuating asymmetry
Authors: Manuta Nicoleta , Duro Sokol , Szara Tomasz , Jashari Tefik , Demircioğlu İsmail , Avanus Kozet , Büyükünal Serkan Kemal 
Published: May 2024

AbstractSheep (Ovis aries) play an important role in the economy of Turkey and the Balkan Peninsula due to their use in farming. As a domesticated species, sheep's morphometric and morphological diversity is likely determined by selective breeding practices rather than geographic distribution. This study aimed to analyse four different sheep breed skulls and reveal skull asymmetry using geometric morphometric methods. For this purpose, 2D images of 52 sheep skulls from different breeds were analysed from the dorsal view of the skull, using 28 landmarks. In the comparison of sheep skulls from the dorsal view, the first principal components for directional asymmetry (DA) and fluctuating asymmetry (FA) were 32.98% and 39.62% of the total variation, respectively. Sharri and Ivesi (Awassi) sheep breeds had the broadest distribution of skull shapes among the breeds, while Lara e Polisit was the most conservative breed. DA was used as a measure of biomechanical constraints, and FA was used as an indicator of environmental stress. Consistent with DA, both differences in centroid size and shape between breeds were statistically significant. No differences between males and females related to asymmetry were revealed. Ivesi sheep revealed the highest fluctuating asymmetry. Geometric morphometric methods proved to be a useful tool for distinguishing differences in the shape of the skull of different sheep breeds and also can be useful for taxonomic purposes.