bluuebunny/crossref_metadata_2025_split huggingface dataset:
Abnormal social reward processing in autism as indexed by pupillary responses to happy faces
Authors: Sepeta Leigh , Tsuchiya Naotsugu , Davies Mari S , Sigman Marian , Bookheimer Susan Y , Dapretto Mirella 
Published: June 2012

AbstractBackgroundIndividuals with Autism Spectrum Disorders (ASD) typically show impaired eye contact during social interactions. From a young age, they look less at faces than typically developing (TD) children and tend to avoid direct gaze. However, the reason for this behavior remains controversial; ASD children might avoid eye contact because they perceive the eyes as aversive or because they do not find social engagement through mutual gaze rewarding.MethodsWe monitored pupillary diameter as a measure of autonomic response in children with ASD (n = 20, mean age = 12.4) and TD controls (n = 18, mean age = 13.7) while they looked at faces displaying different emotions. Each face displayed happy, fearful, angry or neutral emotions with the gaze either directed to or averted from the subjects.ResultsOverall, children with ASD and TD controls showed similar pupillary responses; however, they differed significantly in their sensitivity to gaze direction for happy faces. Specifically, pupillary diameter increased among TD children when viewing happy faces with direct gaze as compared to those with averted gaze, whereas children with ASD did not show such sensitivity to gaze direction. We found no group differences in fixation that could explain the differential pupillary responses. There was no effect of gaze direction on pupil diameter for negative affect or neutral faces among either the TD or ASD group.ConclusionsWe interpret the increased pupillary diameter to happy faces with direct gaze in TD children to reflect the intrinsic reward value of a smiling face looking directly at an individual. The lack of this effect in children with ASD is consistent with the hypothesis that individuals with ASD may have reduced sensitivity to the reward value of social stimuli.
Premature expression of a muscle fibrosis axis in chronic HIV infection
Authors: Kusko Rebecca L , Banerjee Camellia , Long Kimberly K , Darcy Ariana , Otis Jeffrey , Sebastiani Paola , Melov Simon , Tarnopolsky Mark , Bhasin Shalender , Montano Monty 
Published: June 2012

Abstract

Background
Despite the success of highly active antiretroviral therapy (HAART), HIV infected individuals remain at increased risk for frailty and declines in physical function that are more often observed in older uninfected individuals. This may reflect premature or accelerated muscle aging.

Methods
Skeletal muscle gene expression profiles were evaluated in three uninfected independent microarray datasets including young (19 to 29 years old), middle aged (40 to 45 years old) and older (65 to 85 years old) subjects, and a muscle dataset from HIV infected subjects (36 to 51 years old). Using Bayesian analysis, a ten gene muscle aging signature was identified that distinguished young from old uninfected muscle and included the senescence and cell cycle arrest gene p21/Cip1 (CDKN1A). This ten gene signature was then evaluated in muscle specimens from a cohort of middle aged (30 to 55 years old) HIV infected individuals. Expression of p21/Cip1 and related pathways were validated and further analyzed in a rodent model for HIV infection.

Results
We identify and replicate the expression of a set of muscle aging genes that were prematurely expressed in HIV infected, but not uninfected, middle aged subjects. We validated select genes in a rodent model of chronic HIV infection. Because the signature included p21/Cip1, a cell cycle arrest gene previously associated with muscle aging and fibrosis, we explored pathways related to senescence and fibrosis. In addition to p21/Cip1, we observed HIV associated upregulation of the senescence factor p16INK4a (CDKN2A) and fibrosis associated TGFβ1, CTGF, COL1A1 and COL1A2. Fibrosis in muscle tissue was quantified based on collagen deposition and confirmed to be elevated in association with infection status. Fiber type composition was also measured and displayed a significant increase in slow twitch fibers associated with infection.

Conclusions
The expression of genes associated with a muscle aging signature is prematurely upregulated in HIV infection, with a prominent role for fibrotic pathways. Based on these data, therapeutic interventions that promote muscle function and attenuate pro-fibrotic gene expression should be considered in future studies.
Molecular dynamics and mutational analysis of the catalytic and translocation cycle of RNA polymerase
Authors: Kireeva Maria L , Opron Kristopher , Seibold Steve A , Domecq Céline , Cukier Robert I , Coulombe Benoit , Kashlev Mikhail , Burton Zachary F 
Published: June 2012

Abstract

Background
During elongation, multi-subunit RNA polymerases (RNAPs) cycle between phosphodiester bond formation and nucleic acid translocation. In the conformation associated with catalysis, the mobile “trigger loop” of the catalytic subunit closes on the nucleoside triphosphate (NTP) substrate. Closing of the trigger loop is expected to exclude water from the active site, and dehydration may contribute to catalysis and fidelity. In the absence of a NTP substrate in the active site, the trigger loop opens, which may enable translocation. Another notable structural element of the RNAP catalytic center is the “bridge helix” that separates the active site from downstream DNA. The bridge helix may participate in translocation by bending against the RNA/DNA hybrid to induce RNAP forward movement and to vacate the active site for the next NTP loading. The transition between catalytic and translocation conformations of RNAP is not evident from static crystallographic snapshots in which macromolecular motions may be restrained by crystal packing.

Results
All atom molecular dynamics simulations of Thermus thermophilus (Tt) RNAP reveal flexible hinges, located within the two helices at the base of the trigger loop, and two glycine hinges clustered near the N-terminal end of the bridge helix. As simulation progresses, these hinges adopt distinct conformations in the closed and open trigger loop structures. A number of residues (described as “switch” residues) trade atomic contacts (ion pairs or hydrogen bonds) in response to changes in hinge orientation. In vivo phenotypes and in vitro activities rendered by mutations in the hinge and switch residues in Saccharomyces cerevisiae (Sc) RNAP II support the importance of conformational changes predicted from simulations in catalysis and translocation. During simulation, the elongation complex with an open trigger loop spontaneously translocates forward relative to the elongation complex with a closed trigger loop.

Conclusions
Switching between catalytic and translocating RNAP forms involves closing and opening of the trigger loop and long-range conformational changes in the atomic contacts of amino acid side chains, some located at a considerable distance from the trigger loop and active site. Trigger loop closing appears to support chemistry and the fidelity of RNA synthesis. Trigger loop opening and limited bridge helix bending appears to promote forward nucleic acid translocation.
Induction of the human cathelicidin LL-37 as a novel treatment against bacterial infections
Authors: van der Does Anne M , Bergman Peter , Agerberth Birgitta , Lindbom Lennart 
Published: June 2012

AbstractReview on endogenous antimicrobial peptide induction by (phenyl)butyrate and vitamin D3 as a new anti-infective strategy.As traditional antibiotics gradually become inefficient, there is a high demand for development of anti-infectives with a mechanism of action that is different from existing antibiotics. Current antibiotics target the pathogen directly, thereby contributing to the selection of multidrug-resistant bacterial strains. AMPs, such as the human cathelicidin LL-37, are small cationic peptides that are part of host defense. They eliminate microbes through diverse mechanisms, thereby contributing to resolution of infections and maintenance of epithelial barrier function. The multiplicity of these mechanisms of action might be a key to restrict the development of resistant bacterial strains. The discovery of LL-37-inducing components, such as butyrate and vitamin D3, has opened new avenues to prevent or treat infections. Butyrate and vitamin D3 are potent inducers of LL-37 but in addition, have many other effects on host immunity. Here, we summarize current data on the effects that LL-37 and its inducers display on the innate immune response and discuss the feasibility for development of these inducers as possible drugs to prevent or treat infections.
L’épigramme de Posidippe sur la statue de Kairos,
AP XVI (Plan.) 275: Image, texte, réalité
Authors: Pordomingo Francisca 
Published: June 2012

Abstract
Epigram AP XVI (Plan.) 275, by Posidippus, contains an ecphrasis of a statue created by the sculptor Lysippus that is an allegory of Kairos – not in expository form but rather in the form of a dialogue of questions and answers, with the aim of revealing the hidden meaning of its peculiar iconographic features. Lysippus’ statue and Posidippus’ epigram are the oldest testimonies existing in art and in the literary sources for Kairos, which was the subject of other descriptions and of a rich iconography starting at the end of the Hellenistic age. They contain discrepancies in the selection of the features characterizing the concept, which possibly derive from a change in the meaning and the role of the allegory. The text provides an indication of the frequency with which certain abstractions were personified and deified in the Hellenistic age, sometimes to the point of becoming an object of worship.
Der englische Phaon und Ovids Sappho (Her. 15). Elijah Fentons Transformation eines Mythos
Authors: Drews Friedemann 
Published: June 2012

Abstract
Elijah Fenton’s (1683–1730) Phaon to Sappho has so far never been in the focus of literary criticism. The article aims at a more detailed understanding of Fenton’s technique of literary reception by means of a close reading of both Phaon to Sappho and Fenton’s model, the (presumably) Ovidian Epistula Sapphus. As will be seen, Fenton offers an unexpected perspective on the ancient myth through the eyes of ‘his’ Phaon: Phaon did not leave Sappho out of faithlessness but was completely unable to entertain any feelings of true love, because he had been cursed by Venus for being unfaithful to his former girl-friend in Malea. Phaon merely enjoyed Sappho’s gift of song as a temporary remedy for the agony within his cursed mind, but had to leave her whenever she stopped singing. At this point Phaon is asking for Sappho’s intercession with Venus on his behalf. Although Fenton makes crucial changes to the plot, he does neither ‘deconstruct’ nor ‘correct’ the myth as such: instead, he meticulously retains all the major motifs developed in Ovid’s epistle and thus achieves a literary transformation of the myth, enabling the reader to regard either perspective on the two protagonists’ relationship as logically possible.
L’opus florentissimum di Alessandro Severo
Authors: Rudoni Elia R. 
Published: June 2012

Abstract
The opus florentissimum mentioned at Aurelius Victor, Liber de Caesaribus 24, 5 is to be identified with the thermae Alexandrianae built by Alexander Severus. An emendation is put forward for the textual problem celebrio in the same passage.
Geographic variations in the frequency of thyroid disorders and thyroid peroxidase antibodies in persons without former thyroid disease within Germany
Authors: Meisinger C , Ittermann T , Wallaschofski H , Heier M , Below H , Kramer A , Döring A , Nauck M , Völzke H 
Published: June 2012

ObjectiveTo investigate regional variations in the frequency of undiagnosed thyroid disorders among 25- to 88-year-old men and women in two communities in the northeast and the south of Germany. In addition, autoantibodies to thyroperoxidase (TPO-Abs) and urinary iodine excretion were determined.MethodsTwo population-based surveys of men and women using a common standardized protocol, the Study of Health in Pomerania (SHIP) in the northeast of Germany (2505 participants) and the Kooperative Gesundheitsforschung in der Region Augsburg (KORA) in the south of Germany (2316 participants), were compared with regard to the frequency of undiagnosed thyroid disorders.ResultsCompared with the northeast of Germany, urinary iodine excretion and serum thyroid-stimulating hormone (TSH) levels were significantly higher in the south. The median urinary iodine concentration was 110 μg/l (64; 169 μg/l) in SHIP and 151 μg/l (97; 214 μg/l) in KORA, and the median TSH value was 0.81 mIU/l (0.56; 1.15 mIU/l) in SHIP and 1.22 mIU/l (0.84; 1.80 mIU/l) in KORA. The frequency of elevated TSH (TSH ≥2.12 mIU/l) was 4.3% in SHIP and 14.1% in KORA (P<0.001); the corresponding values for suppressed TSH (<0.25 mIU/l) were 3.5 and 1.7% (P<0.001). The proportion of ultrasonographic findings was 55.5% in SHIP and 68.0% in KORA. The frequency of serum TPO-Abs did not differ significantly between northeast and south Germany.ConclusionsThere were considerable regional disparities in the frequency of thyroid disorders within Germany. These differences can be explained not only by different regional histories of natural iodine deficiency but also by current differences in the iodine supply under an identical nationwide iodine fortification program.
The 2012 Thomas Hunt Morgan Medal
Authors: Wolfner Mariana F , Schedl Tim 
Published: June 2012

Abstract
The Genetics Society of America annually honors members who have made outstanding contributions to genetics. The Thomas Hunt Morgan Medal recognizes a lifetime contribution to the science of genetics. The Genetics Society of America Medal recognizes particularly outstanding contributions to the science of genetics over the past 31 years. The George W. Beadle Medal recognizes distinguished service to the field of genetics and the community of geneticists. The Elizabeth W. Jones Award for Excellence in Education recognizes individuals or groups who have had a significant, sustained impact on genetics education at any level, from kindergarten through graduate school and beyond. The Novitski Prize recognizes an extraordinary level of creativity and intellectual ingenuity in solving significant problems in biological research through the application of genetic methods. We are pleased to announce the 2012 awards.
The Centenary of Janssens’s Chiasmatype Theory
Authors: Koszul Romain , Meselson Matthew , Van Doninck Karine , Vandenhaute Jean , Zickler Denise 
Published: June 2012

Abstract
The segregation and random assortment of characters observed by Mendel have their basis in the behavior of chromosomes in meiosis. But showing this actually to be the case requires a correct understanding of the meiotic behavior of chromosomes. This was achieved only gradually, over several decades, with much dispute and confusion along the way. One crucial step in the understanding of meiosis was provided in 1909 by Frans Alfons Janssens who published in La Cellule an article entitled “La théorie de la Chiasmatypie. Nouvelle interprétation des cinèses de maturation,” which contains the first description of the chiasma structure. He observed that, of the four chromatids present at the connection sites (chiasmata sites) at diplotene or anaphase of the first meiotic division, two crossed each other and two did not. He therefore postulated that the maternal and paternal chromatids that crossed penetrated the other until they broke and rejoined in maternal and paternal segments new ways; the other two chromatids remained free and thus intact. This allowed him also to propose that the chromatids distributed in the four nuclei issued from the second meiotic division had various combinations of maternal and paternal segments of each chromosome. And conversely, permitted the appreciation that the laws of Mendelian segregation required breakage and joining (crossing over) between homologous non-sister chromatids. Although Janssens’s article found a broad appreciative audience and had a large influence on the chromosomal theory at that time, his theory was resisted by both geneticists and cytologists for several decades. This Perspectives aims to highlight the novelty of Janssens’s chiasmatype theory by examining the historical background and our actual understanding of meiotic recombination.
Education, Genetic Ancestry, and Blood Pressure in African Americans and Whites
Authors: Non Amy L. , Gravlee Clarence C. , Mulligan Connie J. 
Published: June 2012

Objectives. We assessed the relative roles of education and genetic ancestry in predicting blood pressure (BP) within African Americans and explored the association between education and BP across racial groups.Methods. We used t tests and linear regressions to examine the associations of genetic ancestry, estimated from a genomewide set of autosomal markers, and education with BP variation among African Americans in the Family Blood Pressure Program. We also performed linear regressions in self-identified African Americans and Whites to explore the association of education with BP across racial groups.Results. Education, but not genetic ancestry, significantly predicted BP variation in the African American subsample (b = −0.51 mm Hg per year additional education; P = .001). Although education was inversely associated with BP in the total population, within-group analyses showed that education remained a significant predictor of BP only among the African Americans. We found a significant interaction (b = 3.20; P = .006) between education and self-identified race in predicting BP.Conclusions. Racial disparities in BP may be better explained by differences in education than by genetic ancestry. Future studies of ancestry and disease should include measures of the social environment.
Urban Sprawl, Physical Activity, and Body Mass Index: Nurses’ Health Study and Nurses’ Health Study II
Authors: James Peter , Troped Philip J. , Hart Jaime E. , Joshu Corinne E. , Colditz Graham A. , Brownson Ross C. , Ewing Reid , Laden Francine 
Published: June 2012

Objectives. We evaluated the association between the county sprawl index, a measure of residential density and street accessibility, and physical activity and body mass index (BMI). Methods. We conducted a multilevel cross-sectional analysis in a sample of Nurses’ Health Study participants living throughout the United States in 2000 to 2001 (n = 136 592). Results. In analyses adjusted for age, smoking status, race, and husband’s education, a 1-SD (25.7) increase in the county sprawl index (indicating a denser, more compact county) was associated with a 0.13 kilograms per meters squared (95% confidence interval [CI] = −0.18, −0.07) lower BMI and 0.41 (95% CI = 0.17, 0.65) more metabolic equivalent (MET) hours per week of total physical activity, 0.26 (95% CI = 0.19, 0.33) more MET hours per week of walking, and 0.47 (95% CI = 0.34, 0.59) more MET hours per week of walking, bicycling, jogging, and running. We detected potential effect modification for age, previous disease status, husband’s education level (a proxy for socioeconomic status), and race. Conclusions. Our results suggest that living in a dense, compact county may be conducive to higher levels of physical activity and lower BMI in women.
Race, Place, and Obesity: The Complex Relationships Among Community Racial/Ethnic Composition, Individual Race/Ethnicity, and Obesity in the United States
Authors: Kirby James B. , Liang Lan , Chen Hsin-Jen , Wang Youfa 
Published: June 2012

Objectives. We explored the association between community racial/ethnic composition and obesity risk. Methods. In this cross-sectional study, we used nationally representative data from the Medical Expenditure Panel Survey linked to geographic data from the US Decennial Census and Census Business Pattern data. Results. Living in communities with a high Hispanic concentration (≥ 25%) was associated with a 0.55 and 0.42 increase in body mass index (BMI; defined as weight in kilograms divided by the square of height in meters) and 21% and 23% higher odds for obesity for Hispanics and non-Hispanic Whites, respectively. Living in a community with a high non-Hispanic Asian concentration (≥ 25%) was associated with a 0.68 decrease in BMI and 28% lower odds for obesity for non-Hispanic Whites. We controlled for individual- and community-level social, economic, and demographic variables. Conclusions. Community racial/ethnic composition is an important correlate of obesity risk, but the relationship differs greatly by individual race/ethnicity. To better understand the obesity epidemic and related racial/ethnic disparities, more must be learned about community-level risk factors, especially how built environment and social norms operate within communities and across racial/ethnic groups.
Racial and Ethnic Disparities in Utilization of Dental Services Among Children in Iowa: The Latino Experience
Authors: Valencia Alejandra , Damiano Peter , Qian Fang , Warren John J. , Weber-Gasparoni Karin , Jones Michael 
Published: June 2012

Objectives. We sought to understand the role of Latino acculturation in dental care utilization in Iowa children. Methods. We used logistic regression to evaluate factors associated with having a previous-year dental check-up with 2005 Iowa Child and Family Household Health Survey data. We constructed models to examine the association with race/ethnicity and used chosen interview language to measure Latino acculturation. Results. After we controlled for several factors, having a regular dental care source, having a dental need, dental insurance status, family income, children's dental health rating, children's age, and brushing habits were associated with having a previous-year dental check-up. Race/ethnicity was indirectly associated with use of dental services through other related factors with significant differences for less-acculturated Latinos. Conclusions. Policymakers and health planners should implement strategies to address individual, community, and system factors affecting racial/ethnic minorities. A regular source of dental care for Latino children that will enhance their access to services should be ensured. Ignoring the needs of the fastest growing segment of children with the poorest oral health and the least access to care will lead to future increase of oral diseases in this population.
Socioeconomic Status and the Black–White Mortality Crossover
Authors: Sautter Jessica M. , Thomas Patricia A. , Dupre Matthew E. , George Linda K. 
Published: June 2012

Objectives. We investigated associations among age, race, socioeconomic status (SES), and mortality in older persons and whether low SES contributes to the Black–White mortality crossover (when elevated age–specific mortality rates invert). Methods. We used panel data from the North Carolina Established Populations for Epidemiologic Studies of the Elderly to test the main and interactive effects of SES on mortality. Results. Discrete-time hazard models showed that the association between low education and mortality did not vary by race or age and was only significant for men. For women, the effect of low income diminished with age and had little impact on the crossover. For men, low income varied by race and age, altering the Black–White crossover and producing low–high income crossovers at advanced ages. Conclusions. Low education and income were associated with increased mortality risk for older adults, but only low income had a differential impact on the Black–White mortality crossover. A primary route to reducing mortality differentials in later life is to prevent the disproportionate selective mortality of Blacks and the poor earlier in the life course.