bluuebunny/crossref_metadata_2025_split huggingface dataset:
Standardization in paediatric echocardiographic reporting and critical interpretation of measurements, functional parameters, and prediction scores: a clinical consensus statement of the European Association of Cardiovascular Imaging of the European Society of Cardiology and the Association for European Paediatric and Congenital Cardiology
Authors: Cantinotti Massimiliano , Di Salvo Giovanni , Voges Inga , Raimondi Francesca , Greil Gerald , Ortiz Garrido Almudena , Bharucha Tara , Grotenhuis Heynric B , Köstenberger Martin , Bonnello Beatrice , Miller Owen , McMahon Colin J , Bohbot Yohann , Cikes Maja , Petrescu Aniela Monica , Stankovic Ivan , Buechel Emanuela Valsangiacomo , Eidem Benjamin , Pignatelli Ricardo H 
Published: June 2024

Abstract
This document has been developed to provide a guide for basic and advanced reporting in paediatric echocardiography. Furthermore, it aims to help clinicians in the interpretation of echocardiographic measurements and functional data for estimating the severity of disease in different paediatric age groups. The following topics will be reviewed and discussed in the present document: (i) the general principle in constructing a paediatric echocardiographic report, (ii) the basic elements to be included, and (iii) the potential and limitation of currently employed tools used for disease severity quantification during paediatric reporting. A guide for the interpretation of Z-scores will be provided. Use and interpretation of parameters employed for quantification of ventricular systolic function will be discussed. Difficulties in the adoption of adult parameters for the study of diastolic function and valve defects at different ages and pressure and loading conditions will be outlined, with pitfalls for the assessment listed. A guide for careful use of prediction scores for complex congenital heart disease will be provided. Examples of basic and advanced (disease-specific) formats for reporting in paediatric echocardiography will be provided. This document should serve as a comprehensive guide to (i) structure a comprehensive paediatric echocardiographic report; (ii) identify the basic morphological details, measures, and functional parameters to be included during echocardiographic reporting; and (iii) correctly interpret measurements and functional data for estimating disease severity.
The generation of stable transgenic lines in the human-infective nematode Strongyloides stercoralis
Authors: Patel Ruhi , Bryant Astra S , Castelletto Michelle L , Walsh Breanna , Akimori Damia , Hallem Elissa A 
Published: June 2024

Abstract
The skin-penetrating gastrointestinal parasitic nematode Strongyloides stercoralis causes strongyloidiasis, which is a neglected tropical disease that is associated with severe chronic illness and fatalities. Unlike other human-infective nematodes, S. stercoralis cycles through a single free-living generation and thus serves as a genetically tractable model organism for understanding the mechanisms that enable parasitism. Techniques such as CRISPR/Cas9-mediated mutagenesis and transgenesis are now routinely performed in S. stercoralis by introducing exogenous DNA into free-living adults and then screening their F1 progeny for transgenic or mutant larvae. However, transgenesis in S. stercoralis has been severely hindered by the inability to establish stable transgenic lines that can be propagated for multiple generations through a host; to date, studies of transgenic S. stercoralis have been limited to heterogeneous populations of transgenic F1 larvae. Here, we develop an efficient pipeline for the generation of stable transgenic lines in S. stercoralis. We also show that this approach can be used to efficiently generate stable transgenic lines in the rat-infective nematode Strongyloides ratti. The ability to generate stable transgenic lines circumvents the limitations of working with heterogeneous F1 populations, such as variable transgene expression and the inability to generate transgenics of all life stages. Our transgenesis approach will enable novel lines of inquiry into parasite biology, such as transgene-based comparisons between free-living and parasitic generations.
Future-proofing genomic data and consent management: a comprehensive review of technology innovations
Authors: Oliva Adrien , Kaphle Anubhav , Reguant Roc , Sng Letitia M F , Twine Natalie A , Malakar Yuwan , Wickramarachchi Anuradha , Keller Marcel , Ranbaduge Thilina , Chan Eva K F , Breen James , Buckberry Sam , Guennewig Boris , Haas Matilda , Brown Alex , Cowley Mark J , Thorne Natalie , Jain Yatish , Bauer Denis C 
Published: June 2024

AbstractGenomic information is increasingly used to inform medical treatments and manage future disease risks. However, any personal and societal gains must be carefully balanced against the risk to individuals contributing their genomic data. Expanding our understanding of actionable genomic insights requires researchers to access large global datasets to capture the complexity of genomic contribution to diseases. Similarly, clinicians need efficient access to a patient’s genome as well as population-representative historical records for evidence-based decisions. Both researchers and clinicians hence rely on participants to consent to the use of their genomic data, which in turn requires trust in the professional and ethical handling of this information.Here, we review existing and emerging solutions for secure and effective genomic information management, including storage, encryption, consent, and authorization that are needed to build participant trust. We discuss recent innovations in cloud computing, quantum-computing-proof encryption, and self-sovereign identity. These innovations can augment key developments from within the genomics community, notably GA4GH Passports and the Crypt4GH file container standard. We also explore how decentralized storage as well as the digital consenting process can offer culturally acceptable processes to encourage data contributions from ethnic minorities.We conclude that the individual and their right for self-determination needs to be put at the center of any genomics framework, because only on an individual level can the received benefits be accurately balanced against the risk of exposing private information.
Experimental investigation of pore-filling substitution effect on frequency-dependent elastic moduli of Berea sandstone
Authors: He Y -X , Wang S X , Tang G Y , Dong C H , Sun C , Yuan S Y , Shi P D 
Published: June 2024

SUMMARY
Based on both forced oscillation and ultrasonic pulse transmission methods, we investigated solid pore infill influences on rock elastic moduli in a broad frequency range $[ {1 - 3000,\,\,{{10}^6}} ]$ Hz for different differential pressures. For a Berea sandstone sample, filled sequentially by solid (${22\,\,^{\rm{o}}}{\rm{C}}$), quasi-solid (${26\,\,^{\rm{o}}}{\rm{C}}$) and liquid (${34\,\,^{\rm{o}}}{\rm{C}}$) octadecane, a frequency-dependence was found for the Poisson's ratio, Young's modulus and bulk modulus, nevertheless, these elastic parameters were strongly suppressed by increasing pressures. Experimental measurements showed that shear wave velocity and modulus of solid-octadecane-filled samples are significantly larger than those of the dry and liquid-octadecane-filled ones, implying the potential stiffening effects related to solid infill in compliant pores. A three porosity structure model, which describes the solid stiffening effects related to equant, compliant and the intermediate pores with aspect ratios larger than those of compliant pores but much less than those of stiff pores, was used to compare against the experimentally measured elastic properties for octadecane pore infill, together with several other fluid/solid substitution theories. The agreement between experimental measurements and theoretical predictions is reasonably good for the sandstone tested, providing that the three porosity model can be applied for pressure- and frequency-dependent elastic moduli estimations for a viscoelastic pore-infill-saturated sandstone. Evaluating the combined squirt flow mechanism responsible for the observed moduli dispersion and attenuation is of great importance to reduce potential errors in seismic AVO inversion and 4-D seismic monitoring of gas-hydrate or bitumen-saturated reservoir, especially for reservoir rocks with complex microstructures and heterogeneous pore types.
Subsurface tidal gravity variation and gravimetric factor
Authors: Rogister Y , Hinderer J , Riccardi U , Rosat S 
Published: June 2024

SUMMARY
Taking advantage of the simultaneous recording during 471 d between 2019 and 2021 by two superconducting gravimeters installed at the surface and 520 m under the surface at the Low Noise Underground Laboratory (LSBB) in Rustrel, France, we investigate whether a difference between the tidal gravity signals at the two locations can be detected. First, we model the periodical variations of the Earth’s gravity owing to the tidal influence from the Sun and Moon, at the Earth’s surface and at shallow depths. We provide analytical formulae for the Love numbers, gravimetric factor and gravity variation of simple spherical planetary models. We also numerically compute those parameters and function for a realistic spherical Earth model. We find that the fractional difference between the semi-diurnal tidal gravity variations at the surface and 520 m below is as small as 8.5 × 10$^{-5}$. We next evaluate the effect on the amplitude of the recorded gravity signal due to the calibration factors of the two superconducting gravimeters at LSBB. Finally, we compute the spectra of the difference between the gravity variations measured on and under the surface in the semi-diurnal band of the M$_2$ tidal wave. We find that the uncertainties associated to the calibration factors are larger than the theoretical or observational difference between the tidal gravity variations on the surface and at a 520-m depth.
Joint inversion of induced polarization and hydraulic tomography data for hydraulic conductivity imaging
Authors: Römhild Lukas , Fiandaca Gianluca , Bayer Peter 
Published: June 2024

SUMMARY
For accurate modelling of groundwater flow and transport processes within an aquifer, precise knowledge about hydraulic conductivity K and its small-scale heterogeneities is fundamental. Methods based on pumping tests, such as hydraulic tomography (HT), allow for retrieving reliable K-estimates, but are limited in their ability to image structural features with high resolution, since the data from time-consuming hydraulic tests are commonly sparse. In contrast, geophysical methods like induced polarization (IP) can potentially yield structural images of much higher resolution, but depend on empirical petrophysical laws that may introduce significant uncertainties to the K-estimation. Therefore, this paper presents a joint inversion procedure for both HT and IP data, which allows for combining the complementary abilities of both methods. Within this approach, a traveltime inversion is applied to the HT data, while the IP inversion is based on a full-decay time-domain forward response, as well as a reparametrization of the Cole–Cole model to invert for K directly. The joint inversion is tested on a synthetic model mimicking horizontally layered sediments, and the results are compared with the individual HT and IP inversions. It is shown that jointly inverting both data sets consistently improves the results by combining the complementary sensitivities of the two methods, and that the inversion is more robust against changes in the experimental setups. Furthermore, we illustrate how a joint inversion approach can correct biases within the petrophysical laws by including reliable K-information from hydraulic tests and still preserving the high-resolution structural information from IP. The different inversion results are compared based on the structural similarity index (SSIM), which underlines the robustness of the joint inversion compared to using the data individually. Hence, the combined application of HT and IP within field surveys and a subsequent joint inversion of both data sets may improve our understanding of hydraulically relevant subsurface structures, and thus the reliability of groundwater modelling results.
AVM: A Manually Curated Database of Aerosol-transmitted Virus Mutations, Human Diseases, and Drugs
Authors: Mei Lan , Hou Yaopan , Zhou Jiajun , Chang Yetong , Liu Yuwei , Wang Di , Zhang Yunpeng , Ning Shangwei , Li Xia 
Published: June 2024

Abstract
Aerosol-transmitted viruses possess strong infectivity and can spread over long distances, earning the difficult-to-control title. They cause various human diseases and pose serious threats to human health. Mutations can increase the transmissibility and virulence of the strains, reducing the protection provided by vaccines and weakening the efficacy of antiviral drugs. In this study, we established a manually curated database (termed AVM) to store information on aerosol-transmitted viral mutations (VMs). The current version of the AVM contains 42,041 VMs (including 2613 immune escape mutations), 45 clinical information datasets, and 407 drugs/antibodies/vaccines. Additionally, we recorded 88 human diseases associated with viruses and found that the same virus can target multiple organs in the body, leading to diverse diseases. Furthermore, the AVM database offers a straightforward user interface for browsing, retrieving, and downloading information. This database is a comprehensive resource that can provide timely and valuable information on the transmission, treatment, and diseases caused by aerosol-transmitted viruses (http://www.bio-bigdata.center/AVM).
The Interface Between Patents and Regulatory Exclusivities and the View on the New EU Proposals Concerning Patent Compulsory Licensing and Regulatory Exclusivities
Authors: Valtere Laura 
Published: June 2024

Abstract
Exclusivity rights such as patents have gained special attention in the post-pandemic period. Patents are intended to be a tool to prevent potential market failure in intangible goods and encourage investment in innovation. At the same time, recently they have been accused of blocking access to COVID-19 vaccines and have even fuelled public debate on the introduction of various measures to limit the exclusivity rights arising from the grant of a patent. However, patents are not the only exclusivity available to (bio)pharmaceuticals. Regulatory exclusivities, though less well-known to broader society, are no less important than patents and no less relied on by the pharmaceutical industry. A fortiori, de lege lata regulatory exclusivities have no exceptions, which means they are more robust than patents and can potentially provide even stronger protection than that afforded by patents.
This contribution provides a comparative view on patents and the less explored ‘8+2+1’ regulatory exclusivities, outlining common and distinctive features and the interaction between the two regimes. Some preliminary reflections are made on potential legislative adjustments, without which the much-debated compulsory licensing may prove to be of little use. Finally, insights are provided into the European Commission’s proposals of spring 2023 for general pharmaceutical legislation and compulsory licensing. Some preliminary insights into the proposed changes to the regulatory exclusivity system are also provided. Special attention is paid to the proposal aimed to reach compatibility between regulatory exclusivities and compulsory licensing.
Follow the money: a global analysis of funding dynamics for global health security
Authors: Robertson Hailey , Graeden Ellie , Kerr Justin , Van Maele Michael , Katz Rebecca 
Published: June 2024

Abstract
Global financing for health security was dramatically impacted by COVID-19. Here, we provide an empirical analysis of how that funding changed. Using data from Global Health Security (GHS) Tracking (tracking.ghscosting.org), we analyzed disbursements of direct financial assistance for GHS from 2016 to 2022 to compare pre-pandemic funding (2016-2019) to post-pandemic funding (2020-2022) for preparedness and response during each of the seven World Health Organization-declared public health emergencies of international concern (PHEICs) from 2009 to 2022. Over $165B was disbursed for capacity-building and preparedness activities between January 2016 and December 2022, and over $76B was provided for PHEIC response. Preparedness funding remained evenly distributed since 2016 across regions, with the African region receiving about 70% of total preparedness funding. Indeed, how capacity-building and preparedness funding is distributed has changed remarkably little since 2016, despite unprecedented changes to the funding environment—including markedly increased spending—in response to COVID-19. This suggests we now have a unique opportunity to restructure how funds are tracked for accountability and assessing return on investment moving forward.
Kinetics and Durability of Antibody and T-Cell Responses to SARS-CoV-2 in Children
Authors: Files Megan A , Gentles Lauren , Kehoe Leanne , Adler Amanda , Lacombe Kirsten , Dickerson Jane A , Greninger Alexander , Waghmare Alpana , Fairlie Tarayn , Pringle Kimberly , Midgley Claire M , Hagen Melissa Briggs , Englund Janet A , Seshadri Chetan 
Published: June 2024

Abstract

Background
The kinetics and durability of T-cell responses to SARS-CoV-2 in children are not well characterized. We studied a cohort of children aged 6 months to 20 years with COVID-19 in whom peripheral blood mononuclear cells and sera were archived at approximately 1, 6, and 12 months after symptom onset.

Methods
We compared antibody responses (n = 85) and T-cell responses (n = 30) to nucleocapsid (N) and spike (S) glycoprotein over time across 4 age strata: 6 months to 5 years and 5–9, 10–14, and 15–20 years.

Results
N-specific antibody responses declined over time, becoming undetectable in 26 (81%) of 32 children by approximately 1 year postinfection. Functional breadth of anti-N CD4+ T-cell responses also declined over time and were positively correlated with N-antibody responses (Pearson r = .31, P = .008). CD4+ T-cell responses to S displayed greater functional breadth than N in unvaccinated children and, with neutralization titers, were stable over time and similar across age strata. Functional profiles of CD4+ T-cell responses against S were not significantly modulated by vaccination.

Conclusions
Our data reveal durable age-independent T-cell immunity to SARS-CoV-2 structural proteins in children over time following COVID-19 infection as well as S-antibody responses in comparison with declining antibody responses to N.
The delegation of discretionary power in international agreements: New comparative evidence from the association of Southeast Asian Nations
Authors: Pham Hung , Thomson Robert 
Published: June 2024

Abstract
According to the transaction-costs perspective on delegation, decision-makers grant more discretion to implementers in relation to policies that are more complex and therefore require more specialist expertise to implement. Furthermore, decision-makers grant less delegation to implementers when those implementers have divergent preferences and are therefore more costly to monitor. The transaction-costs perspective has implications for the design of international agreements, such as those adopted by the European Union (EU) and the Association of Southeast Asian Nations (ASEAN). While delegation in the EU has been the subject of systematic research, delegation in ASEAN has not. We argue that ASEAN offers a particularly hard test of the transaction-costs perspective, especially in relation to its propositions concerning implementers’ policy preferences. Notwithstanding the unique characteristics of ASEAN, the evidence provides strong support for the transaction-costs perspective both in terms of specialist expertise and implementers’ preferences. The new dataset we examine includes information on more than 8,500 major provisions within the 235 legal instruments adopted by ASEAN since 1967.
Design of patient-facing immunization visualizations affects task performance: an experimental comparison of 4 electronic visualizations
Authors: Marquard Jenna , Austin Robin , Rajamani Sripriya 
Published: June 2024

Abstract

Objective
This study experimentally evaluated how well lay individuals could interpret and use 4 types of electronic health record (EHR) patient-facing immunization visualizations.

Materials and Methods
Participants (n = 69) completed the study using a secure online survey platform. Participants viewed the same immunization information in 1 of 4 EHR-based immunization visualizations: 2 different patient portals (Epic MyChart and eClinicWorks), a downloadable EHR record, and a clinic-generated electronic letter (eLetter). Participants completed a common task, created a standard vaccine schedule form, and answered questions about their perceived workload, subjective numeracy and health literacy, demographic variables, and familiarity with the task.

Results
The design of the immunization visualization significantly affected both task performance measures (time taken to complete the task and number of correct dates). In particular, those using Epic MyChart took significantly longer to complete the task than those using eLetter or eClinicWorks. Those using Epic MyChart entered fewer correct dates than those using the eLetter or eClinicWorks. There were no systematic statistically significant differences in task performance measures based on the numeracy, health literacy, demographic, and experience-related questions we asked.

Discussion
The 4 immunization visualizations had unique design elements that likely contributed to these performance differences.

Conclusion
Based on our findings, we provide practical guidance for the design of immunization visualizations, and future studies. Future research should focus on understanding the contexts of use and design elements that make tables an effective type of health data visualization.
A computationally supported designer benzodiazepine strategy for public toxicology laboratories
Authors: Ciallella Heather L , Taruvinga Danai T , Yacoub Kimberly , Sofalvi Szabolcs , Delor Samantha M , Kaspar Claire K , Mitchell-Mata Christie L , Travaglianti Shelby , Lavins Eric S , Apollonio Luigino G 
Published: June 2024

Abstract
Public laboratories must balance innovative and existing methods to keep up with designer drug trends. This article presents a strategy for handling designer benzodiazepines (DBZDs) in casework from screening to interpretation. The cross-reactivity of 22 DBZDs and metabolites was tested against the Immunalysis™ benzodiazepine (BZD) direct enzyme-linked immunosorbent assay kit. The kit had high intra-analyte precision (coefficients of variation &lt;15%). Inter-analyte performance varied, triggering confirmation testing at concentrations ranging from 35 to 460 μg/L. The Cuyahoga County Regional Forensic Science Laboratory implemented a 40-analyte BZD and Z-drug confirmation method in 2019. Ten additional analytes were later validated for qualitative reporting, and the limits of detection for 13 analytes were lowered by 60%. The method of standard addition was also optimized for as-needed quantitation. Equal and 1/x weighting factors correlated well with target concentrations (coefficients of determination (r2) &gt; 0.98), but 1/x weighting provided the most consistently accurate concentrations. Six computational models were developed to predict γ-aminobutyric acid-A receptor binding affinity to assist in case interpretation (r2 &gt; 0.70 for cross-validation and test set prediction). These models were used to predict the binding affinity of analytes in the confirmation method. Other public laboratories can use this same practical strategy to adapt to any designer drug class (e.g., BZDs, opioids, cannabinoids and stimulants).