bluuebunny/crossref_metadata_2025_split huggingface dataset:
The differential assimilation of nitrogen fertilizer compounds by soil microorganisms
Authors: Charteris Alice F , Knowles Timothy D J , Mead Andrew , Reay Michaela K , Michaelides Katerina , Evershed Richard P 
Published: June 2024

Abstract
The differential soil microbial assimilation of common nitrogen (N) fertilizer compounds into the soil organic N pool is revealed using novel compound-specific amino acid (AA) 15N-stable isotope probing. The incorporation of fertilizer 15N into individual AAs reflected the known biochemistry of N assimilation—e.g. 15N-labelled ammonium (15NH4+) was assimilated most quickly and to the greatest extent into glutamate. A maximum of 12.9% of applied 15NH4+, or 11.7% of ‘retained’ 15NH4+ (remaining in the soil) was assimilated into the total hydrolysable AA pool in the Rowden Moor soil. Incorporation was lowest in the Rowden Moor 15N-labelled nitrate (15NO3−) treatment, at 1.7% of applied 15N or 1.6% of retained 15N. Incorporation in the 15NH4+ and 15NO3− treatments in the Winterbourne Abbas soil, and the 15N-urea treatment in both soils was between 4.4% and 6.5% of applied 15N or 5.2% and 6.4% of retained 15N. This represents a key step in greater comprehension of the microbially mediated transformations of fertilizer N to organic N and contributes to a more complete picture of soil N-cycling. The approach also mechanistically links theoretical/pure culture derived biochemical expectations and bulk level fertilizer immobilization studies, bridging these different scales of understanding.
An RNAi screen to identify proteins required for cohesion rejuvenation during meiotic prophase in Drosophila oocytes
Authors: Haseeb Muhammad A , Bernys Alana C , Dickert Erin E , Bickel Sharon E 
Published: June 2024

Abstract
Accurate chromosome segregation during meiosis requires the maintenance of sister chromatid cohesion, initially established during premeiotic S phase. In human oocytes, DNA replication and cohesion establishment occur decades before chromosome segregation and deterioration of meiotic cohesion is one factor that leads to increased segregation errors as women age. Our previous work led us to propose that a cohesion rejuvenation program operates to establish new cohesive linkages during meiotic prophase in Drosophila oocytes and depends on the cohesin loader Nipped-B and the cohesion establishment factor Eco. In support of this model, we recently demonstrated that chromosome-associated cohesin turns over extensively during meiotic prophase and failure to load cohesin onto chromosomes after premeiotic S phase results in arm cohesion defects in Drosophila oocytes. To identify proteins required for prophase cohesion rejuvenation but not S phase establishment, we conducted a Gal4-UAS inducible RNAi screen that utilized two distinct germline drivers. Using this strategy, we identified 29 gene products for which hairpin expression during meiotic prophase, but not premeiotic S phase, significantly increased segregation errors. Prophase knockdown of Brahma or Pumilio, two positives with functional links to the cohesin loader, caused a significant elevation in the missegregation of recombinant homologs, a phenotype consistent with premature loss of arm cohesion. Moreover, fluorescence in situ hybridization confirmed that Brahma, Pumilio, and Nipped-B are required during meiotic prophase for the maintenance of arm cohesion. Our data support the model that Brahma and Pumilio regulate Nipped-B-dependent cohesin loading during rejuvenation. Future analyses will better define the mechanism(s) that govern meiotic cohesion rejuvenation and whether additional prophase-specific positives function in this process.
Surface conductivity of clays
Authors: Emelianov Viacheslav , Zhang Zeyu , Weller Andreas , Halisch Matthias , Titov Konstantin 
Published: June 2024

SUMMARY
Clay minerals are extensively used in a wide range of applications. In particular, clay-bearing formations are considered as suitable radioactive waste repository. Electrical resistivity tomography is an appropriate tool to monitor the properties of clay-bearing locations. However, an inherent drawback of a conventional resistivity survey is its ambiguity in distinguishing between the effects of groundwater salinity, clay content and porosity. A discrimination can be achieved on the basis of the induced polarization method that provides a complex conductivity. The main purpose of this study is the investigation of the complex conductivity of clay samples with a special focus on the contribution of surface conductivity produced by an excess of ions in the electrical double-layer coating the solid particles. Six clay mixtures were selected that include an almost pure kaolinite sample, a sample consisting of a mixture of kaolinite, illite and smectite, a crushed saponite breccia, a Ca-bentonite sample and two illite clay samples. Besides the enriched kaolinite, the other samples are natural geomaterials that contain more than 40 weight per cent clay minerals. The mineralogical compositions of the samples were determined by quantitative X-ray diffraction analysis. The clay powder was mixed with a varying volume of sodium chloride solution to get plastic state clay samples with varying water content. The samples were investigated by the spectral induced polarization method in a frequency range between 1 mHz and 1 kHz. The resulting complex conductivity spectra indicate a decrease of the real part of the electrical conductivity with rising water content for the illite, bentonite and saponite breccia samples. The overall conductivity of these clay samples is dominated by their surface conductivity. In contrast, the electrical conductivity of kaolinite and kaolinite–illite mixture does not show any significant changes with the water content. For all samples, the imaginary part of electrical conductivity increases at low water content. The real part of the surface conductivity indicates a linear dependence on the volumetric clay content. The slope of this linear relationship can be used to distinguish the types of clay. The ratio between imaginary conductivity and surface conductivity, which decreases with increasing clay content, proves to be a suitable parameter that characterizes the connectivity of clay aggregates in the sample. The surface conductivity of the pure kaolinite sample has been determined in an additional multisalinity experiment. The resulting surface conductivity is in good agreement with the experiment of varying water content. The multisalinity experiment has shown that the resulting petrophysical parameters depend on the procedure of sample packing, which may lead to anisotropy. The effect of anisotropy is attributed to the alignment of the plate-like kaolinite particles in the course of the packing and consolidation procedure.
The 2022 ICSID Arbitration Rules: Modernizing International Investment Dispute Resolution
Authors: Torterola Ignacio , Sharipov Farhod 
Published: June 2024

Abstract
The new Arbitration Rules of the International Center for Settlement of Investment Disputes (ICSID), which came into force on July 1, 2022, replace the previous version in force since 2006. These rules seek to make the ICSID arbitration process more efficient and responsive, while maintaining the high standards that have made ICSID a respected forum for resolving investment disputes. The new rules reflect changes in international investment law and policy over the past decade, and also aim to address shortcomings of the previous system, such as lack of transparency and efficiency. New provisions have been introduced to simplify and speed up arbitration procedures, provide greater transparency, increase the legitimacy of arbitration processes and expand access to them. The new rules reflect the expectations of ICSID and its members for more efficient and transparent resolution of investment disputes and should facilitate the further growth of international investment and trade, as well as the resolution of disputes arising in connection with these processes.
Burden of overweight and obesity among adolescents in Ethiopia: a systematic review and meta-analysis
Authors: Eyeberu Addis , Dessie Yadeta , Assefa Nega 
Published: June 2024

Abstract

Background
Overweight and obesity continue to escalate, despite the regulations and interventions that have been put in place. To support further policy directions, it is necessary to have pooled data that illustrate the burden of overweight and obesity among adolescents nowadays. Thus, this systematic review and meta-analysis aimed to determine the burden of overweight and obesity among Ethiopian adolescents.

Methods
Embase, Science Direct, Medline, PubMed, Google Scholar and Web of Science were the databases used in the search for publications that ran from inception until 25 October 2023. STATA version 18 was used to analyze data on overweight and obesity in adolescents. Using the random effect model, the pooled estimates with 95% CIs were calculated. Using the Galbraith plot, I2 statistics and Cochrane Q statistics, heterogeneity was investigated.

Results
In total, 20145 adolescents aged 10–19 years were included in this meta-analysis study. The pooled prevalence of overweight and obesity among adolescents in Ethiopia was 10% (95% CI 9 to 12%; I2=94.03%). There was a substantial correlation between being female (OR=1.81, 95% CI 1.47 to 2.15), attending private schools (OR=1.78, 95% CI 1.31 to 2.26), having a family size of ≥4 (OR=1.86, 95% CI 1.14 to 2.58) and being of middle economic status (OR=1.62, 95% CI 1.14 to 2.10).

Conclusions
In Ethiopia, one in 10 adolescents is overweight/obese. The study's findings clearly show that a large number of adolescents are dealing with this emerging public health issue. Moreover, characteristics related to physical activity, nutrition and sociodemographics were discovered to be associated with overweight and obesity. To lessen the burden of overweight and obesity, the government and other parties should work together to support gender-specific nutritional interventions, encourage healthy lifestyle choices among teenagers and facilitate these efforts.
Evolutionary history of tyrosine-supplementing endosymbionts in pollen-feeding beetles
Authors: Wierz Jürgen C , Gimmel Matthew L , Huthmacher Selina , Engl Tobias , Kaltenpoth Martin 
Published: June 2024

Abstract
Many insects feeding on nutritionally challenging diets like plant sap, leaves, or wood engage in ancient associations with bacterial symbionts that supplement limiting nutrients or produce digestive or detoxifying enzymes. However, the distribution, function, and evolutionary dynamics of microbial symbionts in insects exploiting other plant tissues or relying on a predacious diet remain poorly understood. Here, we investigated the evolutionary history and function of the intracellular gamma-proteobacterial symbiont “Candidatus Dasytiphilus stammeri” in soft-winged flower beetles (Coleoptera, Melyridae, Dasytinae) that transition from saprophagy or carnivory to palynivory (pollen-feeding) between larval and adult stage. Reconstructing the distribution of the symbiont within the Dasytinae phylogeny unraveled not only a long-term coevolution, originating from a single acquisition event with subsequent host–symbiont codiversification, but also several independent symbiont losses. The analysis of 20 different symbiont genomes revealed that their genomes are severely eroded. However, the universally retained shikimate pathway indicates that the core metabolic contribution to their hosts is the provisioning of tyrosine for cuticle sclerotization and melanization. Despite the high degree of similarity in gene content and order across symbiont strains, the capacity to synthesize additional essential amino acids and vitamins and to recycle urea is retained in some but not all symbionts, suggesting ecological differences among host lineages. This report of tyrosine-provisioning symbionts in insects with saprophagous or carnivorous larvae and pollen-feeding adults expands our understanding of tyrosine supplementation as an important symbiont-provided benefit across a broad range of insects with diverse feeding ecologies.
USPSTF Recommendations and Overdiagnosis
Authors: Hendrick R Edward , Monticciolo Debra L 
Published: June 2024

Abstract
Overdiagnosis is the concept that some cancers detected at screening would never have become clinically apparent during a woman’s lifetime in the absence of screening. This could occur if a woman dies of a cause other than breast cancer in the interval between mammographic detection and clinical detection (obligate overdiagnosis) or if a mammographically detected breast cancer fails to progress to clinical presentation. Overdiagnosis cannot be measured directly. Indirect methods of estimating overdiagnosis include use of data from randomized controlled trials (RCTs) designed to evaluate breast cancer mortality, population-based screening studies, or modeling. In each case, estimates of overdiagnosis must consider lead time, breast cancer incidence trends in the absence of screening, and accurate and predictable rates of tumor progression. Failure to do so has led to widely varying estimates of overdiagnosis. The U.S. Preventive Services Task Force (USPSTF) considers overdiagnosis a major harm of mammography screening. Their 2024 report estimated overdiagnosis using summary evaluations of 3 RCTs that did not provide screening to their control groups at the end of the screening period, along with Cancer Intervention and Surveillance Network modeling. However, there are major flaws in their evidence sources and modeling estimates, limiting the USPSTF assessment. The most plausible estimates remain those based on observational studies that suggest overdiagnosis in breast cancer screening is 10% or less and can be attributed primarily to obligate overdiagnosis and nonprogressive ductal carcinoma in situ.
Building An Abolition Movement for International Criminal Law?
Authors: Rigney Sophie 
Published: June 2024

Abstract
International criminal law is a carceral system, which responds to mass atrocities by holding some individuals criminally responsible for these events and then, generally, imprisoning those individuals. I ask what alternative responses might be envisaged, and if we can build a carceral abolitionist movement for international criminal law. Such a movement would refuse imprisonment and policing as the main responses to mass atrocity, and instead would seek to understand the social causes and conditions that cause such mass atrocity, and also how (and why) criminal law has become the preeminent ‘legitimate hand of justice’. In this article, I attempt to bring into conversation two particular intellectual movements: the collective scholarship and praxis of domestic carceral abolition movements; and those critical scholars of international criminal law who have already set out some of the limits and possibilities of the field. I set out the structural conditions of international criminal law, particularly its ideological grounding in neoliberalism and its relationships to race, global capital and colonialism and imperialism, which result in a legal system that criminalizes some and exonerates others. I then particularly examine some tools that already exist for building an abolition movement for international criminal law. These include the defence of duress, transformative reparations and the role of defence lawyers. These tools are admittedly part of the carceral system, and therefore cannot be the key to an abolition movement for international criminal law. However, they may assist us in the short or medium term as we imagine and work towards a better world without the need for a carceral system of international criminal law that relies on police and prisons.
Single-cell analysis defines LGALS1+ fibroblasts that promote proliferation and migration of intrahepatic cholangiocarcinoma
Authors: Cao Qiqi , Yang Jinxian , Jiang Lixuan , Yang Zhao , Fan Zhecai , Chen Shuzhen , Zhu Sibo , Yin Lei , Wang Hongyang , Wen Wen 
Published: June 2024

Abstract
The incidence rate of intrahepatic cholangiocarcinoma (ICC), which has a poor prognosis, is rapidly increasing. To investigate the intratumor heterogeneity in ICC, we analyzed single-cell RNA sequencing data from the primary tumor and adjacent normal tissues of 14 treatment-naïve patients. We identified 10 major cell types, along with 45 subclusters of cells. Notably, we identified a fibroblast cluster, Fibroblast_LUM+, which was preferably enriched in tumor tissues and actively interacted with cholangiocytes. LGALS1 was verified as a marker gene of Fibroblast_LUM+, contributing to the malignant phenotype of ICC. Higher amount of LGALS1+ fibroblasts was associated with poorer overall survival of ICC patients. Mechanistically, LGALS1+ fibroblasts activated the proliferation and migration of tumor cells by upregulating the expression levels of CCR2, ADAM15, and β-integrin. Silencing LGALS1 in cancer-associated fibroblasts (CAFs) suppressed CAF-augmented tumor cell migration and invasion in vitro as well as tumor formation in vivo, suggesting that blockade of LGALS1 serves as a potential therapeutic approach for ICC. Taken together, our single-cell analysis provides insight into the interaction between malignant cells and specific subtypes of fibroblasts, which contributes to better understanding of the intratumor heterogeneity in ICC and the development of novel strategies for the treatment of ICC by targeting fibroblasts in the tumor microenvironment.
Philosophical Reflections on Music Therapy Musicianship
Authors: Matney Bill , Fisher CharCarol , Meadows Anthony , Schwartz Elizabeth , Turry Alan 
Published: June 2024

Abstract
This philosophical inquiry critically examines music therapy musicianship in order to reconceptualize the ways in which musicianship is conceived of and taught in education and training programs in the United States. Through a constructive and critical interaction with historical and extant literature, we seek to create space for the uniqueness of musicianship in our field. We challenge the relevance of the conservatory model, the primacy of the work concept, and the focus on fine art often found in educational settings. In doing so, we align music therapy musicianship with relevant musics, instrumentation, and the unique contextual and relational components of music experiences in our work. We construct multidirectional connections between musicality, musical identity, musicianship, music therapy context, musicking, and the clients’/participants’ lifeworld, introducing subconcepts within and between each concept. This co-construction with the literature asserts our identity in education, training, practice, and research. We conclude by offering preliminary guidance that may further develop music therapy musicianship in education and clinical training programs, in alignment with current reports on education.
Empowering Adolescent and Young Adults with SCD by Enhancing Career Choices and Support
Authors: Johnson Shirley A , Hines Raven , Zhang Yue , Sop Daniel M , Smith Wally R 
Published: June 2024

Abstract

Presentation Date: 6/9/2024
Presentation Start Time: 3:30:00 PM

Background
Over 40% of Americans live with at least one chronic illness. Their illness poses unique barriers in social determinants of health, including employment. Initiatives like Chronically Capable have pioneered virtual career fairs to connect adults with disabilities to employment opportunities. However, Sickle Cell Disease adolescents and young adults (SCD AYAs) transferring from pediatric to adult care after high school may be cast into unemployment and indecision about further education, training, or work without these opportunities. Virginia Commonwealth University’s Adult Sickle Cell Medical Home therefore conducted an in-person Transition Career Fair to bridge the communication gap between SCD AYAs needing work, training, or skills, and available educational and workforce opportunities.

Methods
We targeted for attendance community colleges, vocational training organizations, and businesses, as well as 331 VCU SCD AYAs aged 17-30, using email, text, phone calls, and flyers. The event, hosted at a conference center in our outpatient building, featured strategically arranged stations for resume building, job applications, and mock interviews. Attendees were encouraged to actively seek opportunities by visiting each station. Each also received curated grab bags containing essentials for career advancement, alongside access to coaching and invaluable networking opportunities.

Results
Table 1 shows the Transition Career Fair garnered significant attention. Attendees expressed overwhelming gratitude for the tailored support and guidance. Preliminary outcomes were promising. Attendees left the fair equipped with enhanced resumes, having submitted job applications and enrolled in colleges. Notably, the meaningful interactions between businesses and patients fostered their comments about the event’s inclusivity and community support. Furthermore, the fair garnered attention from VCUHS leadership, local media, and the First Lady of Virginia, raising broader awareness.

Conclusions
AYAs with SCD face multifaceted challenges, but initiatives like this Transition Career Fair underscore the power of community collaboration and targeted support in empowering patients to pursue fulfilling career paths. Next steps are to provide the 26 attendees ongoing support and follow-up, to integrate vocational planning seamlessly into patient care, and to ensure that every SCD AYA has the resources and opportunities needed to envision and realize a successful future. We expect that SCD Transition programs dedicated to supporting SCD AYAs in improving social determinants of health will demonstrate better health outcomes related to AYA independence and professional fulfillment.
Outcomes from SCD AYA Transition Career Fair. DARS=Department for Aging and Rehabilitative Services