bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhanced visual processing contributes to matrix reasoning in autism
Authors: Soulières Isabelle , Dawson Michelle , Samson Fabienne , Barbeau Elise B. , Sahyoun Chérif P. , Strangman Gary E. , Zeffiro Thomas A. , Mottron Laurent 
Published: June 2009

AbstractRecent behavioral investigations have revealed that autistics perform more proficiently on Raven's Standard Progressive Matrices (RSPM) than would be predicted by their Wechsler intelligence scores. A widely‐used test of fluid reasoning and intelligence, the RSPM assays abilities to flexibly infer rules, manage goal hierarchies, and perform high‐level abstractions. The neural substrates for these abilities are known to encompass a large frontoparietal network, with different processing models placing variable emphasis on the specific roles of the prefrontal or posterior regions. We used functional magnetic resonance imaging to explore the neural bases of autistics' RSPM problem solving. Fifteen autistic and eighteen non‐autistic participants, matched on age, sex, manual preference and Wechsler IQ, completed 60 self‐paced randomly‐ordered RSPM items along with a visually similar 60‐item pattern matching comparison task. Accuracy and response times did not differ between groups in the pattern matching task. In the RSPM task, autistics performed with similar accuracy, but with shorter response times, compared to their non‐autistic controls. In both the entire sample and a subsample of participants additionally matched on RSPM performance to control for potential response time confounds, neural activity was similar in both groups for the pattern matching task. However, for the RSPM task, autistics displayed relatively increased task‐related activity in extrastriate areas (BA18), and decreased activity in the lateral prefrontal cortex (BA9) and the medial posterior parietal cortex (BA7). Visual processing mechanisms may therefore play a more prominent role in reasoning in autistics. Hum Brain Mapp, 2009. © 2009 Wiley‐Liss, Inc.
Helping families improve: an evaluation of two primary care approaches to parenting support in the Netherlands
Authors: de Graaf Ireen , Onrust Simone , Haverman Merel , Janssens Jan 
Published: June 2009

AbstractThe present study evaluated two primary care parenting interventions. First, we evaluated the most widely used Dutch practices for primary care parenting support. Second, we assessed the applicability of the Primary Care Triple P approach, which is now being utilized in a wide variety of primary care settings. Both interventions target parents of children with mild to moderate behavioural and/or emotional problems, with the aim of improving parenting skills and thereby decreasing child problems. We examined the interventions in pre‐, post‐ and follow‐up assessment, and compared the results. Both interventions produced significant reductions in reported child emotional and behaviour problems, that also remained after 3 months. For both groups, parenting styles were also found to have improved at both post‐test and follow‐up measurement. When compared with the regular Dutch parenting consultation practices, however, the Primary Care Triple P approach produced greater reductions in parental laxness and total parenting dysfunction, and greater improvement in total parenting competence at both post‐test and follow‐up. Primary Care Triple P may even—in light of the greater improvements in parenting skills and total parental competences in the Triple P group than in the regular Dutch parenting consultation group—produce better results in the long run concerning child behaviour and emotional problems. Copyright © 2009 John Wiley & Sons, Ltd.
Continuous and intermittent dosing of lonafarnib potentiates the therapeutic efficacy of docetaxel on preclinical human prostate cancer models
Authors: Liu Gongjie , Taylor Stacey A. , Marrinan Cindy H. , Hsieh Yunsheng , Bishop W. Robert , Kirschmeier Paul , Long Brian J. 
Published: June 2009

AbstractLonafarnib is a potent, selective farnesyltransferase inhibitor (FTI) undergoing clinical studies for the treatment of solid tumors and hematological malignancies. Preclinically, a number of FTIs, including lonafarnib, interact with taxanes to inhibit cancer cell growth in an additive/synergistic manner. These observations provided rationale for investigating the effects of combining lonafarnib and docetaxel on preclinical prostate cancer models. To date, docetaxel is the only chemotherapeutic agent in clinical use for hormone‐refractory prostate cancer. In vitro experiments with 22Rv1, LNCaP, DU‐145, PC3 and PC3‐M prostate cancer cell lines showed significantly enhanced inhibition of cell proliferation and apoptosis when lonafarnib was added to docetaxel. In human tumor xenograft models, continuous coadministration of lonafarnib with docetaxel caused marked tumor regressions (24–47%) in tumors from all of the cell types as well as parental CWR22 xenografts. Intermittent dosing of lonafarnib (5 days on then 5 days off) coadministered with docetaxel produced similar regressions in hormone‐refractory 22Rv1 tumors. 22Rv1 tumors progressing on docetaxel treatment also responded to treatment with intermittent lonafarnib (5 days on then 5 days off). Moreover, animals did not exhibit any signs of toxicity during coadministration of lonafarnib and docetaxel. In conclusion, coadministration of continuous and intermittent lonafarnib enhanced the antitumor activity of docetaxel in a panel of prostate cancer models. An intermittent dosing schedule of lonafarnib coadministered with docetaxel may allow enhanced efficacy to that of continuous dosing by improving the tolerability of higher doses of lonafarnib. © 2009 UICC
Reciprocal changes in gene expression profiles of cocultured breast epithelial cells and primary fibroblasts
Authors: Rozenchan Patricia Bortman , Carraro Dirce Maria , Brentani Helena , Mota Louise Danielle de Carvalho , Bastos Elen Pereira , Ferreira Elisa Napolitano e , Torres Cesar H. , Katayama Maria Lúcia Hirata , Roela Rosimeire Aparecida , Lyra Eduardo C. , Soares Fernando Augusto , Folgueira Maria Aparecida Azevedo Koike , Góes João Carlos Guedes Sampaio , Brentani Maria Mitzi 
Published: June 2009

AbstractThe importance of epithelial‐stroma interaction in normal breast development and tumor progression has been recognized. To identify genes that were regulated by these reciprocal interactions, we cocultured a nonmalignant (MCF10A) and a breast cancer derived (MDA‐MB231) basal cell lines, with fibroblasts isolated from breast benign‐disease adjacent tissues (NAF) or with carcinoma‐associated fibroblasts (CAF), in a transwell system. Gene expression profiles of each coculture pair were compared with the correspondent monocultures, using a customized microarray. Contrariwise to large alterations in epithelial cells genomic profiles, fibroblasts were less affected. In MDA‐MB231 highly represented genes downregulated by CAF derived factors coded for proteins important for the specificity of vectorial transport between ER and golgi, possibly affecting cell polarity whereas the response of MCF10A comprised an induction of genes coding for stress responsive proteins, representing a prosurvival effect. While NAF downregulated genes encoding proteins associated to glycolipid and fatty acid biosynthesis in MDA‐MB231, potentially affecting membrane biogenesis, in MCF10A, genes critical for growth control and adhesion were altered. NAFs responded to coculture with MDA‐MB231 by a decrease in the expression of genes induced by TGFβ1 and associated to motility. However, there was little change in NAFs gene expression profile influenced by MCF10A. CAFs responded to the presence of both epithelial cells inducing genes implicated in cell proliferation. Our data indicate that interactions between breast fibroblasts and basal epithelial cells resulted in alterations in the genomic profiles of both cell types which may help to clarify some aspects of this heterotypic signaling. © 2009 UICC
The degradation of cell cycle regulators by SKP2/CKS1 ubiquitin ligase is genetically controlled in rodent liver cancer and contributes to determine the susceptibility to the disease
Authors: Calvisi Diego F. , Pinna Federico , Ladu Sara , Muroni Maria R. , Frau Maddalena , Demartis Ilaria , Tomasi Maria L. , Sini Marcella , Simile Maria M. , Seddaiu Maria A. , Feo Francesco , Pascale Rosa M. 
Published: June 2009

AbstractPrevious work showed a genetic control of cell cycle deregulation during hepatocarcinogenesis. We now evaluated in preneoplastic lesions, dysplastic nodules and hepatocellular carcinoma (HCC), chemically induced in genetically susceptible F344 and resistant Brown Norway (BN) rats, the role of cell cycle regulating proteins in the determination of a phenotype susceptible to HCC development. p21WAF1, p27KIP1, p57KIP2 and p130 mRNA levels increased in fast growing lesions of F344 rats. Lower/no increases occurred in slowly growing lesions of BN rats. A similar behavior of RassF1A mRNA was previously found in the 2 rat strains. However, p21WAF1, p27KIP1, p57KIP, p130 and RassF1A proteins exhibited no change/low increase in the lesions of F344 rats and consistent rise in dysplastic nodules and HCC of BN rats. Increase in Cks1‐Skp2 ligase and ubiquitination of cell cycle regulators occurred in F344 but not in BN rat lesions, indicating that posttranslational modifications of cell cycle regulators are under genetic control and contribute to determine a phenotype susceptible to HCC. Moreover, proliferation index of 60 human HCCs was inversely correlated with protein levels but not with mRNA levels of P21WAF1, P27KIP1, P57KIP2 and P130, indicating a control of human HCC proliferation by posttranslational modifications of cell cycle regulators.
Participation and study decisions in a public system of higher education
Authors: Kelchtermans Stijn , Verboven Frank 
Published: June 2009

AbstractWe analyze the decision whether to participate and where and what to study in a public system of higher education, based on a unique dataset of all eligible high school pupils in an essentially closed region (Flanders). We find that pupils perceive the available institutions and programs as close substitutes relative to the outside option. This implies an ambiguous role for travel costs: they hardly affect the participation decision, but have a strong impact on the decision where and what to study. To illustrate how our empirical results can inform the debate on reforming public systems, we assess the effects of tuition fee increases. Uniform cost‐based tuition fee increases achieve most of the welfare gains; the additional gains from fee differentiation are relatively limited. These welfare gains are quite large under conservative assumptions on the social cost of public funds, and there is a substantial redistribution from students to outsiders. Copyright © 2009 John Wiley & Sons, Ltd.
Upregulation of gamma‐2 laminin‐332 in the mouse ear vesicant wound model
Authors: Chang Yoke‐Chen , Sabourin Carol L. K. , Lu Shou‐En , Sasaki Takako , Svoboda Kathy K. H. , Gordon Marion K. , Riley David J. , Casillas Robert P. , Gerecke Donald R. 
Published: June 2009

AbstractEpithelial cell migration during wound healing is regulated in part by enzymatic processing of laminin‐332 (formerly LN‐5), a heterodimer formed from α, β, and γ polypeptide chains. Under static conditions, laminin‐332 is secreted into the extracellular matrix as a proform and has two chains processed to smaller forms, allowing it to anchor epithelial cells to the basement membrane of the dermis. During incisional wounding, laminin γ2 chains in particular are processed to smaller sizes and function to promote epithelial sheet migration over the wound bed. The present study examines whether this same function occurs following chemical injury. The mouse ear vesicant model (MEVM) was used to follow the pathology in the ear and test whether processed laminin‐332 enhances epithelial cell migration. Skin biopsies of sulfur mustard (SM) exposed ears for several time points were analyzed by histology, immunohistochemistry, real‐time PCR, and Western blot analysis. SM exposure greatly increased mRNA levels for laminin‐γ2 in comparison to the other two chains. Protein production of laminin‐γ2 was upregulated, and there was an increase in the processed forms. Protein production was in excess of the amount required to form heterotrimeric laminin‐332 and was associated with the migrating epithelial sheet, suggesting a potential role in wound healing for monomeric laminin‐γ2. © 2009 Wiley Periodicals, Inc. J Biochem Mol Toxicol 23:172–184, 2009; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20275
Antioxidant butylated hydroxyanisole inhibits estrogen‐induced breast carcinogenesis in female ACI rats
Authors: Singh Bhupendra , Mense Sarah M. , Remotti Fabrizio , Liu Xinhua , Bhat Hari K. 
Published: June 2009

AbstractExposure to estrogens is suggested to be a risk factor in human breast cancer development. The mechanisms underlying estrogen‐induced cancer have not been fully elucidated. Both estrogen receptor (ER)‐mediated proliferative processes and ER‐independent generation of oxidative stress are suggested to play important roles in estrogen‐induced breast carcinogenesis. In the current study, we investigated the role of oxidative stress in breast carcinogenesis using the ACI rat model of mammary tumorigenesis. Female ACI rats were treated with 17β‐estradiol (E2), butylated hydroxyanisole (BHA), or a combination of E2 + BHA for up to 240 days. Cotreatment of rats with E2 + BHA reduced estrogen‐induced breast tumor development with tumor incidence of 24%, a significant decrease relative to E2 where tumor incidence was 82%. Proliferative changes in the breast tissue of E2 + BHA‐treated animals were similar to those observed in E2‐treated animals. Tissue levels of 8‐isoprostane, a marker of oxidant stress, as well as the activities of antioxidant enzymes including glutathione peroxidase, superoxide dismutase, and catalase were quantified in the breast tissues of rats treated with E2 + BHA and compared to activity levels found in E2‐treated animals and respective age‐matched controls. Cotreatment with BHA inhibited E2‐mediated increases in 8‐isoprostane levels as well as activities of antioxidant enzymes. In summary, these data suggest that estrogen‐mediated oxidant stress plays a critical role in the development of estrogen‐dependent breast cancers and BHA inhibits E2‐dependent breast carcinogenesis by decreasing oxidant stress. © 2009 Wiley Periodicals, Inc. J Biochem Mol Toxicol 23:202–211, 2009; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20281
Oxidative damage and gene expression profile of antioxidant enzymes after T‐2 toxin exposure in mice
Authors: Chaudhari Manjari , Jayaraj R. , Santhosh S. R. , Rao P. V. Lakshmana 
Published: June 2009

AbstractT‐2 toxin is one of the most potent trichothecenes, and on exposure causes severe human and animal diseases. We investigated the dose‐ and time‐dependent effect of T‐2 toxin on certain biochemical variables, oxidative damage in terms of antioxidant enzyme activity, and gene expression profile in mice. Mice treated intraperitoneally with either 1 LD50 or 2 LD50 dose (5.61 and 11.22 mg/kg body weight, respectively) of T‐2 toxin showed significant alterations in hepatic alanine amino transferase, aspartate amino transferase, and lactate dehydrogenase. Significant changes in hepatic lipid peroxidation, depletion of glutathione (GSH), and expression of heat shock protein‐70 indicated oxidative damage. We also evaluated the activity of antioxidant enzymes and compared the gene expression profile by quantitative real‐time reverse transcriptase–polymerase chain reaction. Except for glutathione reductase (GR), there was a significant increase in activity of glutathione‐S‐transferase (GST), glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase at 1 LD50 dose. At 2 LD50 dose, SOD showed decrease in activity, whereas GST, GPx, and catalase showed significant increase. In contrast, gene expression profile showed downregulation in GR, GPx, GST, and catalase at 1 LD50 dose. At 2 LD50 dose except GSH synthetase, all other genes were downregulated. The results clearly show oxidative stress as one of the mechanisms of T‐2 toxin‐mediated toxicity. © 2009 Wiley Periodicals, Inc. J Biochem Mol Toxicol 23:212–221, 2009; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20282
Changes in expression of cell‐cycle‐related genes in PC‐3 prostate cancer cells caused by ovine uterine serpin
Authors: Padua Maria B. , Hansen Peter J. 
Published: June 2009

AbstractThe hormonal‐regulated serpin, ovine uterine serpin (OvUS), also called uterine milk protein (UTMP), inhibits proliferation of lymphocytes and prostate cancer (PC‐3) cells by blocking cell‐cycle progression. The present aim was to identify cell‐cycle‐related genes regulated by OvUS in PC‐3 cells using the quantitative human cell‐cycle RT2 Profiler™ PCR array. Cells were cultured ±200 µg/ml recombinant OvUS (rOvUS) for 12 and 24 h. At 12 h, rOvUS increased expression of three genes related to cell‐cycle checkpoints and arrest (CDKN1A, CDKN2B, and CCNG2). Also, 14 genes were down‐regulated including genes involved in progression through S (MCM3, MCM5, PCNA), M (CDC2, CKS2, CCNH, BIRC5, MAD2L1, MAD2L2), G1 (CDK4, CUL1, CDKN3) and DNA damage checkpoint and repair genes RAD1 and RBPP8. At 24 h, rOvUS decreased expression of 16 genes related to regulation and progression through M (BIRC5, CCNB1, CKS2, CDK5RAP1, CDC20, E2F4, MAD2L2) and G1 (CDK4, CDKN3, TFDP2), DNA damage checkpoints and repair (RAD17, BRCA1, BCCIP, KPNA2, RAD1). Also, rOvUS down‐regulated the cell proliferation marker gene MKI67, which is absent in cells at G0. Results showed that OvUS blocks cell‐cycle progression through upregulation of cell‐cycle checkpoint and arrest genes and down‐regulation of genes involved in cell‐cycle progression. J. Cell. Biochem. 107: 1182–1188, 2009. © 2009 Wiley‐Liss, Inc.
On the kinetic mechanism of the hydrogen abstraction reactions of the hydroxyl radical with CH3CF2Cl and CH3CFCl2: A dual level direct dynamics study
Authors: Ji Yue‐Meng , Cao Fenglei , Gao Hui , Li Xiangzhi , Zhao Cunyuan , Su Chengyong , Liu Jing‐Yao , Li Ze‐Sheng 
Published: June 2009

AbstractBy means of the dual‐level direct dynamics method, the mechanisms of the reactions, CH3CF2Cl + OH → products (R1) and CH3CFCl2 + OH → products (R2), are studied over a wide temperature range 200–2000 K. The optimized geometries and frequencies of the stationary points are calculated at the MP2/6‐311G(d,p) level, and then the energy profiles of the reactions are refined with the interpolated single‐point energy method at the G3(MP2) level. The canonical variational transition‐state theory with the small‐curvature tunneling (SCT) correction method is used to calculate the rate constants. For the title reactions, three reaction channels are identified and the H‐abstraction channel is the major pathway. The results indicate that F substitution has a significant (reductive) effect on hydrochlorofluorocarbon reactivity. Also, for all H‐abstraction reaction channels the variational effect is small and the SCT effect is only important in the lower temperature range on the rate constants calculation. © 2009 Wiley Periodicals, Inc. J Comput Chem, 2010
Excited states of OsO4: A comprehensive time‐dependent relativistic density functional theory study
Authors: Zhang Yong , Xu Wenhua , Sun Qiming , Zou Wenli , Liu Wenjian 
Published: June 2009

AbstractA large number of scalar as well as spinor excited states of OsO4, in the experimentally accessible energy range of 3–11 eV, have been captured by time‐dependent relativistic density functional linear response theory based on an exact two‐component Hamiltonian resulting from the symmetrized elimination of the small component. The results are grossly in good agreement with those by the singles and doubles coupled‐cluster linear response theory in conjunction with relativistic effective core potentials. The simulated‐excitation spectrum is also in line with the available experiment. Furthermore, combined with detailed analysis of the excited states, the nature of the observed optical transitions is clearly elucidated. It is found that a few scalar states of 3T1 and 3T2 symmetries are split significantly by the spin‐orbit coupling. The possible source for the substantial spin‐orbit splittings of ligand molecular orbitals is carefully examined, leading to a new interpretation on the primary valence photoelectron ionization spectrum of OsO4. © 2009 Wiley Periodicals, Inc. J Comput Chem, 2010
Transition state theory with Tsallis statistics
Authors: Quapp Wolfgang , Zech Alraune 
Published: June 2009

AbstractWe discuss the rate of an elementary chemical reaction. We use the reaction path and especially its saddle point on the potential energy surface. The reaction path connects reactant and product of a reaction over the transition state (TS). Usually, the TS is assumed near or at the single saddle point of the reaction path. By means of comparison of the statistics of states at the reactant and at the TS, one can estimate the reaction rate by the Eyring theory. We propose to use the Tsallis statistics at the TS, a statistics of seldom accidents. Thus, we propose to generalize the well‐known Boltzmann–Gibbs statistics, which is the limiting case of the Tsallis statistics. We use features of this nonextensive thermostatistics. The basic properties of the statistics are used to derive (approximated) partition functions, and they are applied on reaction rates. The approximation includes a factorization of the partition functions. The theory is applied to HCN isomerization to HNC, and to the reaction H2 + CN → H + HCN. It allows an accordance with experimental estimations of the reaction rates. © 2009 Wiley Periodicals, Inc. J Comput Chem, 2010
Closed trail decompositions of complete equipartite graphs
Authors: Burgess Andrea , Šajna Mateja 
Published: June 2009

AbstractThe complete equipartite graph \documentclass{article}\footskip=0pc\pagestyle{empty}\begin{document}$K_m * {\overline{K_n}}$\end{document} has mn vertices partitioned into m parts of size n, with two vertices adjacent if and only if they are in different parts. In this paper, we determine necessary and sufficient conditions for the existence of a decomposition of \documentclass{article}\footskip=0pc\pagestyle{empty}\begin{document}$K_m * {\overline{K_n}}$\end{document} into closed trails of length k. © 2009 Wiley Periodicals, Inc. J Combin Designs 17: 374–403, 2009
Authenticating and distinguishing the eight species of traditional Tibetan medicine “Meiduoluomi” by microscopic technique
Authors: An Jing , Li Jie , Wang Jiang‐Gang , Zhang Zhi‐Feng , Chen Chu , Zhang Hao 
Published: June 2009

AbstractBecause of the morphological and macroscopic similarity, many species of Erigeron and Aster (Asteraceae) are confusable and usually used under the same name “Meiduoluomi” in traditional Tibetan medicine (TTM). To find an easy, quick, and reliable method to authenticate and distinguish the eight main medicinal plants of these species, the light microscope was used to reveal the morphoanatomic details. The fixed, sectioned, and stained plant materials and epidermis materials were studied by microscopic techniques. The results of the microscopic features are systematically described and illustrated, and comparison parameters are presented. Furthermore, a key to the eight species of “Meiduoluomi” was constructed. Microscopy can be unambiguously used to authenticate and distinguish the eight main species of TTM “Meiduoluomi.” Microsc. Res. Tech., 2009. © 2009 Wiley‐Liss, Inc.