bluuebunny/crossref_metadata_2025_split huggingface dataset:
Single-port-plus-one robot-assisted laparoscopic Lich–Gregoir direct nipple ureteral extravesical reimplantation in pediatric primary obstructive megaureter, comparing to laparoscopic cohen
Authors: Lin Shan , Xu Huihuang , He Yufeng , Xu Xinru , You Guangxu , Chen Jianglong , Xu Di 
Published: May 2024

Abstract
Purpose
To compare the effects of a single-port-plus-one robotic laparoscopic-modified Lich–Gregoir direct nipple approach and traditional laparoscopic Cohen in treating pediatric primary obstructive megaureter.

Materials and methods
The clinical data of 24 children with primary obstructive megaureter from January 2021 to November 2021 were analyzed retrospectively. Among them, 12 children (8 boys and 4 girls, the average age were 17.17 ± 6.31 months) treated with the laparoscopic Cohen method were defined as group C. The remaining 12 children (7 boys and 5 girls, the average age was 17.33 ± 6.99 months) underwent single-port-plus-one robotic laparoscopic-modified Lich–Gregoir direct nipple ureteral extravesical reimplantation were defined as group L. The parameters of pre-operation, intraoperative and postoperative were compared.

Results
There were no differences in the patient characteristics and average follow-up time between the two groups (P > 0.05).The obstruction resolution rate was 100% in both groups. The total operation time in group L is slightly longer than that in group C(P < 0.001),but the intraperitoneal operation time of the two groups was comparable(P > 0.05). The postoperative parameters included blood loss, gross haematuria time, indwelling catheterization time and hospitalization time in group L is shorter than group C(P < 0.05). One year post-operation, decreasing in ureteral diameter and APRPD, and increasing in DRF were remarkably observed in both two groups(P < 0.05). Ureteral diameter, APRPD, and DRF were not significantly different both in pre-operation and post-operation between Group L and Group C(P > 0.05).

Conclusion
Single-port-plus-one robot-assisted laparoscopic-modified Lich–Gregoir direct nipple approach and traditional laparoscopic Cohen are both dependable techniques for ureteral reimplantation in the treatment of pediatric primary obstructive megaureter. Since Lich–Gregoir can preserve the physiological direction of the ureter and direct nipple reimplantation enhances the effect of anti-refluxing, this technique is favorable for being promoted and applied in robot surgery.
Development of Sustainable Slag-based Geopolymer Concrete Using Different Types of Chemical Admixtures
Authors: Amer Ismail , Abdelkhalik Amr , Mayhoub Ola A. , Kohail Mohamed 
Published: May 2024

AbstractGeopolymer concrete (GPC) has achieved a wide popularity since innovating it as an alternative to conventional concrete because of its superior mechanical characteristics and durability, in addition to being a green concrete due to its low negative impact on the environment. However, GPC still suffers from the problem of its poor workability which suppresses its spread in construction applications. This study investigated the most effective parameters on the workability of GPC including GGBFS content, water to binder ratio, and dosage of different types of chemical admixtures, Naphthalene-Based Admixture (NPA) and Polycarboxylate-Based Admixture (PCA), using Taguchi approach and Analysis of Variance (ANOVA) analysis considering the compressive strength at the different concrete ages. It was observed that NPA, in the geopolymer concrete, improved the compressive strength compared to PCA. The NPA-based mixes achieved the highest 28-day compressive strength, 69 MPa, with about 27.8% more than the highest 28-day compressive strength achieved by the PCA-based mixes, 54 MPa. The obtained results revealed that the NPA has achieved the best improvement for both the workability, in terms of initial slump value and slump loss rate, and the compressive strength of GPC mixes compared to PCA.
Determination of the temporal–spatial distribution patterns of ancient heritage sites in China and their influencing factors via GIS
Authors: Li Xiaojia 
Published: May 2024

Abstract
In this paper, we analysed the temporal and spatial distributions of ancient archaeological sites in China as well as their influencing factors. Our aim was to reveal the developmental trajectory of Chinese civilisation and to explore the natural and cultural factors affecting human distribution, with the goal of providing insights for the conservation and development of ancient relics. We employed spatial analysis methods using ArcGIS 10.8 software, such as kernel density analysis and trend surface analysis, to analyse 1194 historical ancient sites listed in the National Cultural Relics Protection Units of China. The research findings are as follows: (1) the distribution of ancient sites demonstrates an agglomerative spatial pattern. The nearest neighbour index (R < 1) for sites from various historical periods indicates an agglomerative spatial distribution of ancient sites across historical periods, with the clustering degree being relatively poor for sites from the Wei-Jin, Northern and Southern Dynasties and in the Ming and Qing periods. The regions with a concentration of ancient sites are the middle and lower reaches of the Yangtze River and Yellow River Basins. Furthermore, there is a higher abundance of ancient sites in the southeastern region than in the northwestern region. (2) The distribution of ancient sites in different historical periods also exhibits an imbalance, with an overall decreasing trend in the number of ancient sites in China. Notably, more ancient sites were found for the prehistoric and pre-Qin periods. The temporal trend of ancient sites during historical periods follows a trajectory from northeast to northwest, northeast to southwest, etc. (3) The temporal and spatial distributions of ancient sites are influenced by multiple factors, including social development, the natural environment, geographical elements, and socioeconomic and political factors. Finally, based on an understanding of the spatiotemporal distribution pattern of ancient sites and the factors that influence this pattern, recommendations for conservation and development can be proposed and supported by modern technological methods, with the aim of offering insights for the protection and sustainable development of heritage sites.
Establishment of an automatic diagnosis system for corneal endothelium diseases using artificial intelligence
Authors: Qu Jing-hao , Qin Xiao-ran , Xie Zi-jun , Qian Jia-he , Zhang Yang , Sun Xiao-nan , Sun Yu-zhao , Peng Rong-mei , Xiao Ge-ge , Lin Jing , Bian Xiao-yan , Chen Tie-hong , Cheng Yan , Gu Shao-feng , Wang Hai-kun , Hong Jing 
Published: May 2024

AbstractPurposeTo use artificial intelligence to establish an automatic diagnosis system for corneal endothelium diseases (CEDs).MethodsWe develop an automatic system for detecting multiple common CEDs involving an enhanced compact convolutional transformer (ECCT). Specifically, we introduce a cross-head relative position encoding scheme into a standard self-attention module to capture contextual information among different regions and employ a token-attention feed-forward network to place greater focus on valuable abnormal regions.ResultsA total of 2723 images from CED patients are used to train our system. It achieves an accuracy of 89.53%, and the area under the receiver operating characteristic curve (AUC) is 0.958 (95% CI 0.943–0.971) on images from multiple centres.ConclusionsOur system is the first artificial intelligence-based system for diagnosing CEDs worldwide. Images can be uploaded to a specified website, and automatic diagnoses can be obtained; this system can be particularly helpful under pandemic conditions, such as those seen during the recent COVID-19 pandemic.
Multi-institutional validation of a radiomics signature for identification of postoperative progression of soft tissue sarcoma
Authors: Yu Yuan , Guo Hongwei , Zhang Meng , Hou Feng , Yang Shifeng , Huang Chencui , Duan Lisha , Wang Hexiang 
Published: May 2024

Abstract
Background
To develop a magnetic resonance imaging (MRI)-based radiomics signature for evaluating the risk of soft tissue sarcoma (STS) disease progression.

Methods
We retrospectively enrolled 335 patients with STS (training, validation, and The Cancer Imaging Archive sets, n = 168, n = 123, and n = 44, respectively) who underwent surgical resection. Regions of interest were manually delineated using two MRI sequences. Among 12 machine learning-predicted signatures, the best signature was selected, and its prediction score was inputted into Cox regression analysis to build the radiomics signature. A nomogram was created by combining the radiomics signature with a clinical model constructed using MRI and clinical features. Progression-free survival was analyzed in all patients. We assessed performance and clinical utility of the models with reference to the time-dependent receiver operating characteristic curve, area under the curve, concordance index, integrated Brier score, decision curve analysis.

Results
For the combined features subset, the minimum redundancy maximum relevance-least absolute shrinkage and selection operator regression algorithm + decision tree classifier had the best prediction performance. The radiomics signature based on the optimal machine learning-predicted signature, and built using Cox regression analysis, had greater prognostic capability and lower error than the nomogram and clinical model (concordance index, 0.758 and 0.812; area under the curve, 0.724 and 0.757; integrated Brier score, 0.080 and 0.143, in the validation and The Cancer Imaging Archive sets, respectively). The optimal cutoff was − 0.03 and cumulative risk rates were calculated.

Data conclusion
To assess the risk of STS progression, the radiomics signature may have better prognostic power than a nomogram/clinical model.
Novel role of LLGL2 silencing in autophagy: reversing epithelial-mesenchymal transition in prostate cancer
Authors: Hong Geum-Lan , Kim Kyung-Hyun , Kim Yae-Ji , Lee Hui-Ju , Cho Sung-Pil , Han Seung-Yun , Yang Seung Woo , Lee Jong-Soo , Kang Shin-Kwang , Lim Jae-Sung , Jung Ju-Young 
Published: May 2024

Abstract
Purpose
Prostate cancer (PCa) is a major urological disease that is associated with significant morbidity and mortality in men. LLGL2 is the mammalian homolog of Lgl. It acts as a tumor suppressor in breast and hepatic cancer. However, the role of LLGL2 and the underlying mechanisms in PCa have not yet been elucidated. Here, we investigate the role of LLGL2 in the regulation of epithelial-mesenchymal transition (EMT) in PCa through autophagy in vitro and in vivo.

Methods
PC3 cells were transfected with siLLGL2 or plasmid LLGL2 and autophagy was examined. Invasion, migration, and wound healing were assessed in PC3 cells under autophagy regulation. Tumor growth was evaluated using a shLLGL2 xenograft mouse model.

Results
In patients with PCa, LLGL2 levels were higher with defective autophagy and increased EMT. Our results showed that the knockdown of LLGL2 induced autophagy flux by upregulating Vps34 and ATG14L. LLGL2 knockdown inhibits EMT by upregulating E-cadherin and downregulating fibronectin and α-SMA. The pharmacological activation of autophagy by rapamycin suppressed EMT, and these effects were reversed by 3-methyladenine treatment. Interestingly, in a shLLGL2 xenograft mouse model, tumor size and EMT were decreased, which were improved by autophagy induction and worsened by autophagy inhibition.

Conclusion
Defective expression of LLGL2 leads to attenuation of EMT due to the upregulation of autophagy flux in PCa. Our results suggest that LLGL2 is a novel target for alleviating PCa via the regulation of autophagy.
The digital transformation in pharmacy: embracing online platforms and the cosmeceutical paradigm shift
Authors: Almeman Ahmad 
Published: May 2024

AbstractIn the face of rapid technological advancement, the pharmacy sector is undergoing a significant digital transformation. This review explores the transformative impact of digitalization in the global pharmacy sector. We illustrated how advancements in technologies like artificial intelligence, blockchain, and online platforms are reshaping pharmacy services and education. The paper provides a comprehensive overview of the growth of online pharmacy platforms and the pivotal role of telepharmacy and telehealth during the COVID-19 pandemic. Additionally, it discusses the burgeoning cosmeceutical market within online pharmacies, the regulatory challenges faced globally, and the private sector’s influence on healthcare technology. Conclusively, the paper highlights future trends and technological innovations, underscoring the dynamic evolution of the pharmacy landscape in response to digital transformation.
Ultrasound-guided minimally invasive thread release of Guyon’s canal: initial experience in cadaveric specimens
Authors: Jengojan Suren , Sorgo Philipp , Kasprian Gregor , Streicher Johannes , Gruber Gerlinde , Moser Veith , Bodner Gerd 
Published: May 2024

Abstract
Objective
Guyon’s canal syndrome is caused by compression of the ulnar nerve at the wrist, occasionally requiring decompression surgery. In recent times, minimally invasive approaches have gained popularity. The aim of this study was to assess the efficacy and safety of ultrasound-guided thread release for transecting the palmar ligament in Guyon’s canal without harming surrounding structures, in a cadaveric specimen model.

Methods
After ethical approval, thirteen ultrasound-guided thread releases of Guyon’s canal were performed on the wrists of softly embalmed anatomic specimens. Cadavers showing injuries or prior operations at the hand were excluded. Subsequently, the specimens were dissected, and the outcome of the interventions and potential damage to adjacent anatomical structures as well as ultrasound visibility were evaluated with a score from one to three.

Results
Out of 13 interventions, a complete transection was achieved in ten cases (76.9%), and a partial transection was documented in three cases (23.1%). Irrelevant lesions on the flexor tendons were observed in two cases (15.4%), and an arterial branch was damaged in one (7.7%). Ultrasound visibility varied among specimens, but essential structures were delineated in all cases.

Conclusion
Ultrasound-guided thread release of Guyon’s canal has shown promising first results in anatomic specimens. However, further studies are required to ensure the safety of the procedure.

Relevance statement
Our study showed that minimally invasive ultrasound-guided thread release of Guyon’s canal is a feasible approach in the anatomical model. The results may provide a basis for further research and refinement of this technique.

Key points
• In Guyon’s canal syndrome, the ulnar nerve is compressed at the wrist, often requiring surgical release.
• We adapted and tested a minimally invasive ultrasound-guided thread release technique in anatomic specimens.
• The technique was effective; however, in one specimen, a small anatomic branch was damaged.

Graphical Abstract
PMCT-based sex determination using posterior segment of greater sciatic notch in North Indian population
Authors: Kumari Ruchi , Pandit Jay Narayan , Panga Surya Kiran , Tyagi Swati , Yadav Abhishek , Gupta Sudhir Kumar 
Published: May 2024

Abstract
Background
Digital tools, which offer superior accuracy compared to manual metrics, utilize radiological images for noninvasive data collection, providing a convenient means of obtaining skeletal data. The greater sciatic notch exhibits high sexual dimorphism and resistance to damage, and therefore serves as a viable tool for sex determination in poorly preserved skeletons, particularly in scenarios involving mass disasters, highly putrefied, and skeletonized cases. In addition to the width and depth of the greater sciatic notch, the length and angle of the posterior segment are highly specific parameters. This study aims to obtain accurate and standardized values for determining sex by measuring the posterior segment of the greater sciatic notch using postmortem computed tomography with a 16-slice multidetector row computed tomography scanner.

Results
The study revealed that except for depth and the distance between the ischial spine and deepest point, all measured variables of the greater sciatic notch were greater for women than men on both sides. The length of the posterior segment and the posterior angle on both sides showed the highest positive correlation and provided highly significant differences between males and females. These findings not only reinforce the utility of the greater sciatic notch in sex determination but also highlight the potential for more accurate and noninvasive methods in forensic investigations.

Conclusions
Postmortem computed tomography accurately determines sex by measuring the posterior sciatic notch segment, achieving a 90.9% accuracy rate. This study, the first of its kind in India, utilized postmortem computed tomography ruler measurements for sex determination, specifically evaluating the role of the posterior segment dimensions of the greater sciatic notch in the North Indian population.
Cannabidiol’s cytotoxicity in pancreatic cancer is induced via an upregulation of ceramide synthase 1 and ER stress
Authors: Mangal Nagina , Reebye Vikash , Habib Nagy , Sodergren Mikael H. 
Published: May 2024

AbstractPancreatic ductal adenocarcinoma (PDAC) remains one of the most aggressive malignancies with a median 5 year-survival rate of 12%. Cannabidiol (CBD) has been found to exhibit antineoplastic potential and may potentiate the anticancer effects of cytotoxic’s such as gemcitabine. CBD therapy has been linked to de novo synthesis of ceramide. The sphingolipid ceramide is a potent tumour suppressor lipid with roles in apoptosis and autophagy. One of the key players involved is ceramide synthase, an enzyme with six isoforms (CerS1-CerS6), reported to have disease prognostic value. Quantitative real time PCR was used to determine mRNA expression levels of ceramide synthase isoforms, GRP78, ATF4 and CHOP. Western blotting was used to analyze protein expression of these markers and knockdown of CerS1 and GRP78 were applied via an siRNA and confirmed by the two mentioned methods. Mice with PDAC xenografts were injected via intraperitoneal method with drugs and tumours were analysed with flow cytometry and processed using H&E and IHC staining. siRNA knockdown of ceramide synthase 1 (CerS1) and analysis point to evidence of a putative CerS1 dependent pathway driven by CBD in activating endoplasmic reticulum (ER) stress target; GRP78. Upon CBD treatment, CerS1 was upregulated and downstream this led to the GRP78/ATF4/CHOP arm of the unfolded protein response (UPR) pathway being activated. In an in vivo model of PDAC in which CerS1 was not upregulated on IHC, there was no observed improvement in survival of animals, however a reduction in tumour growth was observed in combination chemotherapy and CBD group, indicating further investigations in vivo. These findings provide evidence of a potential ceramide induced cytotoxic mechanism of action of CBD in pancreatic ductal adenocarcinoma.
Presentation of enthesitis-related arthritis and juvenile-onset spondyloarthritis: a cross-sectional study in a pediatric and adult clinic
Authors: Lanças Sean Hideo Shirata , Furlan Matheus Zanata Brufatto , Fernandes Taciana de Albuquerque Pedrosa , Drumond Sula Glaucia Lage , Magalhães Claudia Saad 
Published: May 2024

Abstract
Background
Juvenile idiopathic arthritis (JIA) comprises a whole spectrum of chronic arthritis starting before 16 years of age. The study aims to explore the clinical and demographic descriptors, treatment, and disease progression of enthesitis-related arthritis (ERA) in comparison with juvenile-onset spondyloarthritis (SpA).

Methods
Cross-sectional analysis of consecutive patients in two dedicated clinics, with a single visit and retrospective case-notes review. Arthritis, enthesitis and sacroiliitis were evaluated by scoring disease activity and damage. Continuous variables were reported by median, interquartile range; categorical variables were reported by the frequency comparison of the two groups.

Results
Thirty-three cases were included, being 23 (69.7%) with ERA. The median age at diagnosis was 12.5 y (SpA) vs. 9 y (ERA) (p < 0.01); the time from symptom onset to diagnosis was 5.5 y (SpA) vs. 1.5 y (ERA) (p < 0.03). In both groups, the predominant presentation was a single joint or < 5 lower limb joints and asymmetric involvement, with a high frequency of enthesitis. There was a higher frequency of mid-tarsal and ankle synovitis in the ERA group and hip involvement in those with SpA. The comparison of the frequency of spine symptoms at presentation, 30% SpA vs. 21.7% ERA (p = 0.7), was not significant, and radiographic progression to spinal involvement occurred in 43.5% of ERA patients. The median time for spinal progression and age at onset was 2.2 and 12 y for ERA, and 4 and 16.5 y for SpA, respectively. Activity and damage scores were not significantly different between the groups. Treatment comparison resulted in 91.3% of ERA and 100% SpA being treated, predominantly with NSAIDs in both groups, followed by DMARDs and biologics, with a higher frequency of biologics in SpA.

Conclusion
The main differences were the late diagnoses of SpA, and the hip and spine involvement, with higher frequency of biologic treatment in juvenile-onset SpA compared to ERA.
Gut microbiota response to consumption of milks fermented with specific strains of Lactococcus lactis with hypocholesterolemic effect
Authors: Rendon-Rosales Miguel A. , Méndez-Romero José I. , Hernández-Mendoza Adrián , González-Córdova Aarón F. , Mazorra-Manzano Miguel A. , García Hugo S. , Beltrán-Barrientos Lilia M. , Estrada-Montoya María C. , Vallejo-Cordoba Belinda 
Published: May 2024

AbstractThe alteration of structure and function of gut microbiota (dysbiosis) appears to be a major factor associated with metabolic disorders such as dyslipidemia and subsequent development of cardiovascular diseases. However, the consumption of fermented milks is a promising strategy to enhance health and restore the function of gut microbiota; specifically, in individuals with intestinal dysbiosis and hypercholesterolemia. Therefore, the aim of the present study was to evaluate the potential association between gut microbiota and the hypocholesterolemic effect of fermented milks with Lactococcus lactis NRRL B-571 (FM-571), NRRL B-572 (FM-572) and NRRL B-600 (FM-600) in Sprague–Dawley rats. Fermented milks were administered to hypercholesterolemic Sprague–Dawley rats during seven weeks. At the end of the experimental period, fecal and colonic microbiota were characterized using 16S RNA gene sequencing. Also, the short chain fatty acids (SCFAs) content was quantified in feces. Results showed that a high-cholesterol diet (HCD) altered the bacterial community in both fecal and mucosal samples. The consumption of fermented milks, specifically FM-572 promoted changes in the structure (beta diversity) in fecal, but not in mucosal microbiota. The levels of SCFAs in feces were improved after fermented milks consumption. From all SCFAs, butyrate was negatively correlated with total cholesterol, LDL-C (p < 0.05) and positively correlated with HDL-C (p < 0.05). Furthermore, Ruminococcaceae, Lactobacillaceae, Lachnospiraceae and Oscillospiraceae families, were negatively associated with total cholesterol, LDL-C (p < 0.05) and positively associated with HDL-C (p < 0.05). The abundance of these families was increased in groups treated with fermented milks, particularly with FM-572 (p < 0.05). Thus, the in vivo hypocholesterolemic effect after the consumption of milks fermented with Lactococcus lactis strains may be related with the modulation of fecal microbiota associated with the increase of butyrate-producing bacteria. Furthermore, these associations may suggest that butyrate may influence the cholesterol metabolism, resulting in the decreasing cholesterol levels.
Graphical Abstract