bluuebunny/crossref_metadata_2025_split huggingface dataset:
SERUM FERRITIN CONCENTRATION COMPARED TO OTHER IRON‐STORE VARIABLES IN THE DIAGNOSIS OF IRON DEFICIENCY
Authors: Rybo Elisabeth , Bengtsson Calle , Hallberg Leif , Lindstedt Göran , Lundberg Per‐Arne 
Published: August 2010

ABSTRACTSerum ferritin concentration was determined in 38‐year‐old women representative of the general female population of the same age resident in Gothenburg, Sweden. The assays were carried out on serum samples which had been frozen (‐22° C to ‐28° C) for ten years. The values were related to haematological variables which had been examined at the time of the initial study. The distribution of serum ferritin values was markedly positively skewed. Woman with a serum ferritin concentration within the lowest three decentiles had the heaviest menstrual blood loss, no or only traces of reticuloendothelial iron in sternal bone marrow and high iron absorption of a tracer dose of labelled iron. High correlation coefficients were obtained when correlations were calculated between serum ferritin and transferrin saturation, sideroblast count in bone marrow and iron absorption. The correlation coefficients were usually higher between serum ferritin concentration and the other haematological variables than the correlations between the individual haematological variables. There was good agreement between the prevalence of reticuloendothelial iron in bone marrow and serum ferritin concentration. We conclude that bone marrow examination may be replaced by determination of serum ferritin concentration when screening for iron deficiency in otherwise healthy subjects. This seems to be relevant also in subjects with a history of recent mild infection or current use of oral contraceptives.
Immunologic subsets in B cell lymphomas defined by surface immunoglobulin isotype and complement receptor — Their relationship to survival
Authors: Kvaløy Stein , Langholm Ruth , Kaalhus Olav , Marton Per F. , Høst Herman , Godal Tore 
Published: August 2010

Cell surface marker profiles were studied on cell suspensions from monoclonal B cell lymphomas. Surface immunoglobulin (sIg) was examined in 178 cases, whereas combined data with sIg and receptors for complement (C3) were available in 99/178 cases. The results showed that B cell lymphomas can be divided into distinct immunological subsets according to surface marker expression. Whereas some histologic subgroups (diffuse centroblastic/centrocytic, centrocytic, immunocytoma) (Kiel classification) consisted of few immunological subtypes, others were more heterogeneous (follicular centroblastic/centrocytic, centroblastic, lymphocytic). By combining immunological and histological subgroups, more than 40 phenotypes could be identified; this diversity most likely reflects, the heterogeneity of the B cell compartment.As part of the same study the prognostic significance of cell marker phenotypes was examined. Survival analysis undertaken on 149/178 patients did not uncover any significant relationship between type of heavy or light chain expression, C3 receptor expression and clinical outcome. Our data do not confirm recent findings that the type of immunological phenotype may be of prognostic significance.
Quantification of the distribution of the marginating granulocyte pool in man
Authors: Peters A. M. , Saverymuttu S. H. , Bell R. N. , Lavender J. P. 
Published: August 2010

The kinetics of autologous granulocytes, separated from whole blood and labelled with 111In‐tropolonate with continuous maintenance in plasma, have been studied in man, using a gamma camera and computer, with the aim of quantifying the distribution of the marginating granulocyte pool (MGP). We have used 3 approaches: dynamic gamma camera imaging immediately following i.v. injection of labelled cells, comparison of the activity signal from 111In‐granulocytes with that from previously injected 111In‐labelled red cells and absolute quantification of 111In present in liver, spleen and blood.Deconvolution analysis of the hepatic and peripheral blood time activity curves indicated that hepatic granulocyte transit time was 2.5± 0.14 min. By comparison with 111In red cells, hepatic transit time was calculated to be 7.4± 0.82 that of red cells, which, assuming an hepatic red cell content of 6% that of the total red cell mass, is equivalent to a transit time of 1.8 min. By comparison with 111In red cells, lung granulocyte transit time as a factor of red cel transit time was 5.4± 0.7 at 5 min and 2.5± 0.13 at 40 min after granulocyte injection. Using these kinetic data, in combination with previously published values for splenic granulocyte transit time, it was calculated that, 5 min after injection, the MGP accounted for 54% of the total blood granulocyte pool (TBGP), was 90% filled, and was distributed between spleen (19%), liver (26%), lung (33%) and the remainder of the body (22%). At 40 min, the MGP accounted for 60% of the TBGP, had equilibrated with the circulating granulocyte pool (CGP), and was distributed between the spleen, liver, lung and remainder of the body according to the following respective percentages: 35, 25, 10 and 30.The total granulocyte contents of the spleen and liver calculated on the basis of the kinetic data were 21% and 22% respectiely and in broad agreement with the values, 34 and 23% respectively, calculated from quantitative scanning.It was concluded that about 70% of the body's MGP was present in the spleen, liver and lung. If the MGP is itself 60% of the TBGP then only about 18% of the TBGP marginates in extra‐hepatosplenopulmonary sites.
Therapy‐induced myelodysplasia and secondary leukaemia
Authors: Frisch B. , Bartl R. , Chaichik S. 
Published: August 2010

A study was performed on bone marrow histology in patients before, during and at various time intervals after chemotherapy and/or radiotherapy for solid tumours, Hodgkin's disease, multiple myeloma and acute leukaemias. Alterations in both haematopoietic and stromal compartments (myelodysplasia) were found in varying degrees of severity depending on the type and the time elapsed from the treatment.The tumour‐induced and post‐therapy myelodysplasia, restitution phase, pre‐leukaemic phase, and therapy‐induced leukaemia and aplasia are described. The findings are discussed in the light of current theories of the mechanisms of therapy‐induced myelodysplasia and acute leukaemia.
Primary myelodysplastic syndrome: Treatment of 6 patients with 13‐cis retinoic acid
Authors: Kerndrup Gitte , Bendix‐Hansen Knud , Pedersen Bent , Ellegaard Jørgen , Hokland Peter 
Published: August 2010

6 patients with primary MDS were treated with 20 mg/m2 13‐cis retinoic acid (13‐RA) (RoacutaneR, Roche, Basel) daily for 6 weeks and with an additional 100 mg/m2 daily for 4 weeks (3 patients). 4 patients responded either with an increase in peripheral blood neutrophil count (2 patients) or with a decrease in MPO‐deficient neutrophils (2 patients). These effects were not seen until 3 weeks after the onset of the treatment and they were accompanied by an increase in the growth of day 11 granulocytemonocyte colonies, and, in the patients showing clonal cytogenetic abnormalities, by a disappearance of minor clonal abnormalities during the treatment. These findings may be the result of a stimulation of the growth of normal or only slightly defective bone marrow progenitor cells in combination with a differentiational effect.The clinical side effects were limited to hyperkeratosis and dryness of mucous membranes in 5 patients. I patient developed a dermatitis. None showed signs of hepatotoxicity. Because of the pronounced subjective discomfort experienced by the patients receiving 100 mg/m2, it seems recommendable to treat patients with MDS with low dosages of 13‐RA (10 to 20 mg/m2) for longer periods (3–6 months) in order to evaluate the effect of 13‐RA on the long term basis.
Unilateral ovarian absence in two capuchin monkeys
Authors: Domingues Sheyla F.S. , Almeida Débora V.C. , Sealercio Sarah R.R.A. , Kastelic John P. , Santos Regiane R. 
Published: August 2010

AbstractBackground Ovarian absence is a rare condition with congenital or traumatic origin.Methods Gross and microscopic examination of two capuchin monkeys were performed.Results Case 1 had absence of the right ovary and distal uterine tube, bilateral absence of fimbriae, and a rudimentary uterus. Case 2 had unilateral ovarian absence, but a functional contralateral ovary, and a normal uterus.Conclusions Unilateral ovarian absence was confirmed in two capuchin monkeys for the first time.
Viability of fibroblasts in a novel probiotic storage media
Authors: Çaglar E , Sandalli N , Kuscu OO , Durhan MA , Pisiriciler R , Ak Calıskan E , Kargul B 
Published: August 2010

Abstract –  A number of storage media have been investigated as to their ability to maintain the viability of the periodontal ligament (PDL) cells and thus to permit longer extra‐alveolar periods prior to replantation of avulsed teeth. The aim of the present in vitro study was to evaluate the number of viable PDL cells of avulsed teeth treated by Hank’s Balanced Salt Solutions (HBSS), saline, a novel probiotic solution and milk. Thirty‐six freshly extracted single‐rooted human teeth with closed apices were divided into one of the four experimental groups and two control groups (N = 6 each). The positive and negative controls corresponded to 0 min and an 8‐h dry time respectively. Following extraction, the coronal 3 mm of PDL tissue was scraped with a #15 scalpel to remove cells that might have been damaged. The experimental teeth were dried for 30 min followed by a 45 min immersion in one of the four experimental media. Each experimental tooth, after drying and soaking, was incubated for 30 min with a 2.5 ml solution of 0.2 mg ml−1 of collagenase CLS II and a 2.4 mg ml−1 solution of dispase grade II in phosphate buffer saline (PBS). The cells were then labelled with 0.4% Trypan blue for determination of viability. The teeth stored in positive control demonstrated the highest number of viable PDL cells followed in rank order by HBSS, saline, Lactobacillus reuteri solution and milk. There was no significant difference in the number of viable PDL cells between HBSS, milk, L. reuteri solution and saline. Within the parameters of this study, it appears that probiotic may be able to maintain PDL cell viability as HBSS, milk, or saline.
Effects of Glutathione and Related Compounds on Teratogenicity of 5‐Fluorouracil or Cadmium Hydrochloride in Mice*
Authors: NAYA Masato , YASUDA Mineo 
Published: August 2010

AbstractGlutathione (GSH) is a tripeptide consisting of cysteine, glutamic acid and glycine, and plays an important role in detoxification reactions. In this report, we describe (1) the effects of the depleting agents of GSH such as diethylmaleate (DEM), phorone, and buthionine sulfoximine (BSO) on teratogenicity of 5‐fluorouracil (5‐FU) in mice, (2) the effects of GSH or N‐acetyl‐L‐cysteine (NAC), a precursor of GSH on teratogenicity of 5‐FU or cadmium hydrochloride (Cd) in mice. Pregnant ICR mice were injected intra‐peritoneally (i.p.) with 5‐FU at dose levels of 20, 25, and 30 mg/kg on day 11 of gestation (vaginal plug = day 0). Mice were injected i.p. with DEM, phorone, or BSO 4 to 6 hours before dosing with 5‐FU. Mice were also pretreated intravenously (i.v.) with GSH at dose levels of 150, 300 and 600 mg/kg, or NAC at dose levels of 80, 160, and 320 mg/kg 0.5 to 2 hours before dosing with 5‐FU. In the Cd‐teratogenicity study, mice were injected i.v. with GSH or NAC before dosing with Cd at 3.5 mg/kg i.p. on day 11 of gestation. Pretreatment with DEM, phorone or BSO increased the incidence of oligodactyly induced by 5‐FU, while pretreatment with GSH or NAC decreased the incidences. Pretreatment with GSH or NAC decreased the incidence of cleft palate and abnormal palatal rugae induced by Cd. The results suggest that cysteine plays a key role in the teratogenicity of 5‐FU or Cd in mice.
Association of Nodular Hyperplasia With Resistance to Cinacalcet Therapy for Secondary Hyperparathyroidism in Hemodialysis Patients
Authors: Hirai Takayuki , Nakashima Ayumu , Takasugi Norihisa , Yorioka Noriaki 
Published: August 2010

AbstractThere have been few long‐term prospective studies investigating the effect of cinacalcet on secondary hyperparathyroidism with or without nodular hyperplasia. We examined whether the effect of cinacalcet on secondary hyperparathyroidism differed between patients with or without nodular hyperplasia. Stable hemodialysis patients with secondary hyperparathyroidism resistant to conventional treatment received cinacalcet for 12 months. Based on ultrasonography findings, patients were divided into group S (gland < 500 mm3 without nodular hyperplasia) and group L (gland ≥ 500 mm3 with nodular hyperplasia). Serum levels of intact parathyroid hormone, bone‐specific alkaline phosphatase, osteocalcin, and cross‐linked N‐terminal telopeptide of type 1 collagen were measured. Thirty‐one patients completed the study. The changes of parameters from the baseline did not differ significantly between the two groups after 6 months. However, the percentage reduction of each parameter was significantly smaller in group L compared with group S after 12 months. Nodular hyperplasia is associated with resistance to cinacalcet therapy in patients on chronic dialysis with secondary hyperparathyroidism.
A New Device to Monitor Blood Volume in Hemodialysis Patients
Authors: Yoshida Izumi , Ando Katsunobu , Ando Yasuhiro , Ookawara Susumu , Suzuki Masayuki , Furuya Hiroaki , Iimura Osamu , Takada Daisuke , Kajiya Masaharu , Komada Takanori , Mori Honami , Tabei Kaoru ,   BVM Study Group
Published: August 2010

AbstractWe developed a new optical device (Nikkiso) to assess changes through blood volume monitoring (BVM) during hemodialysis and were able to determine the ideal levels in which changes in blood volume percentage (BV%) occur among hemodialysis patients in one hemodialysis center. We evaluated both the reliability of BVM and these ideal levels in a multicenter group. The purpose of this manuscript is to develop a navigating system to set dry weight in a variety of situations as the final goal. First, based on the obtained BVM (BV%BVM) measurements, the relationships between BV% and hematocrit (BV%HT) and between BV% and CRIT‐LINE (BV%CLM; Hema Metrics, Kaysville, UT, USA) were then evaluated. In 30 hemodialysis patients, there was a close correlation between both BV%BVM vs. BV%HT and BV%BVM vs. BV%CLM (n = 30, r = 0.967, P < 0.001, and n = 36, r = 0.7867, P < 0.001, respectively). Second, BV% data were obtained from 464 treatment cases performed on 26 subjects in one satellite hemodialysis center on patients whose body weight was deemed clinically suitable. The formulas for the levels of BV% (standardized by the percent change in body weight at the end of hemodialysis treatment: BW%end) were determined.Finally, we revalidated the reliability of the above levels. A total of 1126 measurements were performed on 201 patients whose body weights were deemed suitable in seven hemodialysis centers. New ideal levels were then recalculated.We therefore conclude that BVM is a sufficiently accurate method of monitoring BV% in hemodialysis treatment. Most well‐controlled hemodialysis patients display the same pattern of BV%/BW%end. Monitoring BV% during hemodialysis is beneficial for determining dry weight (DW).
Characteristics of 20‐Year Survivors Undergoing Maintenance Hemodialysis
Authors: Morimoto Satoshi , Nishioka Hiroaki , Morita Tatsuyori , Jo Fusakazu , Someya Kazunori , Nakahigashi Mitsutaka , Kusabe Makiko , Ueda Hiroko , Takahashi Nobuyuki , Iwasaka Toshiji , Maki Kei 
Published: August 2010

AbstractHemodialysis techniques have improved remarkably in recent decades and the number of long‐term survivors among patients with end‐stage renal disease has increased. The mortality rate of hemodialysis patients has been reported to be low in Japan. However, the long‐term survival rate of dialysis patients is still low: 23.6% for 15 years and 17.4% for 20 years, even in Japan, and background information on patients undergoing hemodialysis therapy for more than 20 years is scarce in this country. In the present study, we investigated the characteristics of 20‐year survivors undergoing maintenance hemodialysis at our medical center. We compared the characteristics of hemodialysis patients who had survived for more than 20 years after the initiation of hemodialysis with those of patients who started hemodialysis at the same time and had already died. No patient among those who were still alive had diabetes mellitus while 15% of patients who had died had diabetes mellitus at the time of initiation of hemodialysis. Age, cardiothoracic ratio, and serum levels of total cholesterol and triglyceride 6 months after the initiation of hemodialysis, as well as decreases in body weight per year were significantly lower in those who had survived than in those who had died. These results suggest that long‐term hemodialysis survivors are characterized by (i) initiation of hemodialysis at a young age (ii) being free of diabetes mellitus (iii) a well‐controlled cardiothoracic ratio (iv) small successive change in body weight, and (v) being free of hypercholesterolemia and hypertriglyceridemia.
White Blood Cell Count Predicts All‐Cause, Cardiovascular Disease‐Cause and Infection‐Cause One‐Year Mortality of Maintenance Hemodialysis Patients
Authors: Hsu Ching‐Wei , Lin Ja‐Liang , Lin‐Tan Dan‐Tzu , Yen Tzung‐Hai , Chen Kuan‐Hsing 
Published: August 2010

AbstractElevated white blood cell (WBC) counts predict coronary heart disease and all‐cause mortality in the general population. Chronic inflammation and malnutrition are associated with increased risk of cardiovascular death in individuals with chronic kidney disease. In this study, we investigated the association between WBC count with inflammation, malnutrition, and mortality in maintenance hemodialysis (MHD) patients. A total of 959 MHD patients were stratified into four equal sized groups based on WBC count. Demographic, hematological, nutritional and inflammatory markers, and biochemical and dialysis‐related data were obtained for cross‐sectional analysis. All patients were followed for one year to investigate the risks for mortality. The mean WBC count was 6.4 ± 1.8 × 103/µL (range: 2.3–16.3 × 103/µL). Stepwise multiple linear regression analysis indicated a positive correlation between WBC count and inflammation (high‐sensitivity C‐reactive protein > 3 mg/L). Forty‐five patients (4.7%) died within the 1‐year study period. Cox multivariate regression analysis demonstrated that total WBC count significantly predicts 1‐year mortality due to all‐cause (hazard ratio (HR): 1.228, 95% confidence interval (CI): 1.095–1.378; P < 0.001), due to cardiovascular disease (HR: 1.242, 95% CI: 1.046–1.475; P = 0.013) and due to infection (HR: 1.252, 95% CI: 1.066–1.470; P = 0.006). These findings suggest that total WBC count should be measured in future studies that evaluate the clinical outcome of MHD patients and that dialysis patients with elevated WBC counts require further medical attention to reduce risks of mortality.
EFFECT OF TOMATO DECAY PROPORTION ON ERGOSTEROL LEVEL AND SOME TOMATO PULP PROPERTIES
Authors: KADAKAL ÇETIN , TAĞI ŞEREF , ARTIK NEVZAT 
Published: August 2010

ABSTRACT There is a need to develop a chemical method to determine the amount of decayed tomatoes used to produce tomato products. Tomato pulp samples produced from surface decayed tomatoes as sound, 30, 60 and 100% were tested for pH, brix, titrable acidity, Hunter L, a and b, Howard Mold Count (HMC) and ergosterol values. Tomato pomace for each decay portion was tested for HMC and ergosterol. Ergosterol analysis was carried out by high‐pressure liquid chromatography (HPLC). Decay proportion was linearly correlated with HMC (r = 0.97) and ergosterol (r = 0.96) in pulp samples but not to pH and acidity. Only ergosterol measurement was applicable in tomato pomace and correlated well (r = 0.95) with the decay proportions. In general, the color values of the samples produced from 100% decayed tomatoes were high compared with the others. The measurement of ergosterol has the potential to be used for the quality assessment of tomato products made from Red Top tomatoes.
BIOLOGICAL COMPETITION AS A PRESERVING MECHANISM
Authors: GOMBAS D. E. 
Published: August 2010

ABSTRACTBiological competition is one “barrier'’against pathogenic growth which has been recommended for use in refrigerated foods. Historically used in fermented foods, lactic acid bacteria alter foods to preclude growth of pathogens. The primary mechanism of inhibition is pH reduction, although other contributing factors have been described. More recently, various lactic acid bacteria have shown potential for inhibiting spoilage bacteria in refrigerated foods, without pH reduction. Other studies have indicated successful inhibition of pathogenic growth by lactic acid bacteria during temperature‐abuse of non‐fermented, refrigerated foods.
EFFECT OF HYDROGEN PEROXIDE CONCENTRATION AND pH ON INACTIVATION KINETICS OF ESCHERICHIA COLI
Authors: RAFFELLINI SILVIA , GUERRERO SANDRA , ALZAMORA STELLA MARIS 
Published: August 2010

ABSTRACT The inactivation kinetics of Escherichia coli ATCC 35218 in hydrogen peroxide solutions at different concentrations (0–3.00% w/v) and pH values (3.0–7.2) at 25C was studied. Effectiveness of H2O2 solutions against E. coli ATCC 35218 appeared to improve at both higher H2O2 concentrations and more acidic pH values. As the H2O2 level was lowered, the influence of the pH of the solution on the rate of E. coli destruction was more notorious. Most of the inactivation semilogarithmic curves were not linear. Inactivation kinetics was modeled by using a Weibull type distribution of resistances. Quadratic polynomial models were applied to express the effects of H2O2 concentration and pH on the exposure time needed to reduce the microbial population by 5 log.PRACTICAL APPLICATIONSThis study gives useful information to predict the effect of H2O2 treatment on Escherichia coli survival as a basis for understanding the only effects of hydrogen peroxide concentration and pH levels, without considering other factors involved in sanitization such as produce surface characteristics, microorganism attachment features and presence of organic material other than the bacteria.Further similar studies using different E. coli strains and other pathogenic bacteria, biofilms and fresh produce would allow to optimize hydrogen peroxide treatments, avoiding or minimizing adverse effects on quality of fresh produce.
ANTIBACTERIAL ACTIVITY OF ASPONGOPUS VIDUATUS (MELON BUG) OIL
Authors: MUSTAFA N.E.M. , MARIOD A.A. , MATTHÄUS B. 
Published: August 2010

ABSTRACTAspongopus viduatus (melon bug) oil is insect oil used as famine food in western parts of Sudan and has traditional medicinal uses. The antibacterial activities of melon bug crude oil, silicic acid column purified oil and phenolic compounds‐free oil were determined by agar diffusion assay against seven bacterial isolates, four of them are food‐related bacterial species: Staphylococcus aureus, Salmonella enterica serovar Paratyphi, Escherichia coli and Bacillus cereus, and the three other isolates are: B. subtilis, Enterococcus faecalis and Pseudomonas aeruginosa. The main constituents of this oil were examined. The crude oil and the phenolic compounds‐free oil showed high antibacterial activities against some test species while the silicic acid column purified oil showed no antibacterial activity. The study highlights the possibility of using this oil in food preservation.PRACTICAL APPLICATIONSIn this study, the antimicrobial properties and main components of insect oil (Aspongopus viduatus) were identified. The oil has traditional medicinal uses in human and animal skin diseases and is also used for meat dressing before drying in western regions of Sudan. The oil has long stability against rancidity because of its high oleic acid content. This oil in its crude form or phenolic compounds‐free form could be used in meat and meat products preservation processes to control gram‐positive bacteria.