bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oxidative Stress and Insulin Resistance
Authors: Park Kyong , Gross Myron , Lee Duk-Hee , Holvoet Paul , Himes John H. , Shikany James M. , Jacobs David R. 
Published: April 2009

OBJECTIVE
Although cumulative evidence suggests that increased oxidative stress may lead to insulin resistance in vivo or in vitro, community-based studies are scarce. This study examined the longitudinal relationships of oxidative stress biomarkers with the development of insulin resistance and whether these relationships were independent of obesity in nondiabetic young adults.

RESEARCH DESIGN AND METHODS
Biomarkers of oxidative stress (F2-isoprostanes [F2Isop] and oxidized LDL [oxLDL]), insulin resistance (the homeostasis model assessment of insulin resistance [HOMA-IR]), and various fatness measures (BMI, waist circumference, and estimated percent fat) were obtained in a population-based observational study (Coronary Artery Risk Development in Young Adults) and its ancillary study (Young Adult Longitudinal Trends in Antioxidants) during 2000–2006.

RESULTS
There were substantial increases in estimated mean HOMA-IR over time. OxLDL and F2Isop showed little association with each other. Mean evolving HOMA-IR increased with increasing levels of oxidative stress markers (P &lt; 0.001 for oxLDL and P = 0.06 for F2Isop), measured in 2000–2001. After additional adjustment for adiposity, a positive association between oxLDL and HOMA-IR was strongly evident, whereas the association between F2Isop and HOMA-IR was not.

CONCLUSIONS
We observed positive associations between each of two oxidative stress markers and insulin resistance. The association with oxidized LDL was independent of obesity, but that with F2Isop was not.
Ultrastructure and cytochemistry of vitellogenesis in Wenyonia virilis Woodland, 1923 (Cestoda, Caryophyllidea)
Authors: Świderski Zdzisław , Młocicki Daniel , Mackiewicz John , Miquel Jordi , Ibraheem Mohammed , Bruňanská Magdalena 
Published: April 2009

AbstractVitellogenesis in Wenyonia virilis was examined by transmission electron microscopy (TEM), including the cytochemical detection of glycogen at the ultrastructural level with the periodic acid-thiosemicarbazide-silver proteinate (PA-TSC-SP) technique. Mature vitelline follicles have cells in various stages of development, progressing from immature cells of gonial type near the periphery of the follicle to maturing and mature vitellocytes towards the centre. Maturation is characterized by: (1) increase in cell volume; (2) increase in nuclear surface area restoring the N/C (nucleo-cytoplasmic) ratio; (3) nucleolar transformation; (4) extensive development of parallel cisternae of GER, the shell-protein producing units; (5) development of Golgi complexes, engaged in shell-granule/shell-globule formation and packaging; (6) synthesis and storage of glycogen in the cytoplasm; (7) simultaneous, independent formation and storage of intranuclear glycogen; (8) continuous fusion of small shell-granules into larger shell-globules and fusion of these into large shell-globule clusters with a progressive increase in the number and size of the latter; and (9) disintegration of GER in the medial layer of vitellocyte cytoplasm, degenerative changes and accumulation of glycogen and shell-globule clusters within the cytoplasm. The functional significance of numerous shell-globule clusters and the relatively small amount of nuclear and cytoplasmic glycogen is analysed. Unlike vitellogenesis of other caryophyllids, the nuclear glycogen of mature vitellocytes in W. virilis is randomly dispersed in the nucleoplasm and never forms a high central accumulation, the so-called “nuclear vacuole”. The nutritive function of vitellocytes appears greatly reduced in W. virilis, a fact perhaps related to the intrauterine development of the early embryos. The ultrastructure of vitellogenesis in W. virilis is compared with that in other lower cestodes, both monozoic and polyzoic. Conclusions concerning interrelationships of the vitellogenesis pattern of the ultrastructural cytochemistry of mature vitellocytes of W. virilis to intrauterine embryonation, absence of uterine glands and an extensive uterus characteristic for this species, are drawn and discussed.
Genetic markers to gastrointestinal nematode resistance in sheep: a review
Authors: Krawczyk A. , Słota E. 
Published: April 2009

Abstract
Nematode parasites are the major animal health constraint in sheep production on pasture and cause serious economic losses. Because of failure of anthelmintic drenches, a major research effort has been underway to examine alternatives to chemical control. One of them is selecting sheep which are genetically resistant to parasitic nematodes. However, this last is not widely practiced because of the difficulty of measuring parasite resistance which mostly relies on indirect criteria such as number of nematode eggs passed in the sheep faeces (FEC) packed cell volume (PCV) or enhanced number of eosinophils in peripheral blood. Despite the well known host immune reaction it has been impossible to standardize any immunological parameter and use it as an indicator of parasitic infection. The aim of finding some genetic markers associated with resistance/susceptibility to nematodes is to make diagnostic work easier and conduct an earlier selection of desirable genotypes. However, searching for reliable genetic markers is rather difficult due to different sheep’s manifestation of resistance to either the adult or larval stages of the same parasite species and against the same parasitic stage and various manifestations of the immune responses and antigens against parasites. This review summarizes findings reported in the literature relating to genetic markers to gastrointestinal nematodes resistance in sheep.
Activity of four entomopathogenic nematode species against different developmental stages of Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera, Chrysomelidae)
Authors: Trdan S. , Vidrih M. , Andjus L. , Laznik Ž. 
Published: April 2009

Abstract
Four entomopathogenic nematode species (Steinernema feltiae, S. carpocapsae, Heterorhabditis bacteriophora, and H. megidis) were tested in a laboratory bioassay for the efficacy of these pathogens in controlling the larvae and adults of the Colorado potato beetle, Leptinotarsa decemlineata. The main aim of the study was to develop an efficient sustainable control method against the pest. With this we could develop a strategy of potato production with the intention of diminishing or even preventing the appearance of pest resistance to insecticides. The activity of these biological agents was assessed at three different temperatures (15, 20, and 25 °C) and three concentrations (200, 1000, and 2000 infective juveniles per individual). Mortality of three stages (young and old larvae and adults) was determined 2, 4, and 7 days after treatment. At 15 °C entomopathogenic nematodes showed the lowest efficacy against all insect stages. No significant differences in efficacy was determined at 20 and 25 °C as all nematodes caused prompt death of all stages. At all temperatures young larvae were most susceptible. However, when controlling overwintered adults for the purpose of preventing the mass appearance of Colorado potato beetle, we recommend an application of higher concentrations of S. feltiae suspension.
Influence of the genetic pattern and sex of mice in experimental lagochilascariosis
Authors: Prudente M. , Freitas J. , Ribeiro E. , Carvalhaes M. 
Published: April 2009

Abstract
We studied experimental lagochilascariosis in male and female mice of four strains. The survival ratio and number of larvae recovered varied: B10.A and C57BL/6 mice had the highest number of larvae 90 days after infection and the lowest survival ratio at 345 days of infection. BALB/c mice had an intermediate survival ratio and number of larvae. A/J mice had the lowest number of larvae and the highest survival ratio. Our findings suggest that resistance to Lagochilascaris minor is not linked to the H-2a region because both susceptible B10.A and resistant A/J mice express the H-2a haplotype. However, the pattern of mortality and larvae recovered that we observed in C57BL/6 mice, which possess the same genetic background as B10.A, indicates that the background genotype does affect the outcome of lagochilascariosis in mice. This study demonstrates that the genetic background, but not H-2a or sex, determine the outcome of lagochilascariosis in mice.
First report of the genus Heterorhabditis (Nematoda: Heterorhabditidae) from continental Portugal
Authors: Valadas V. , Vieira P. , Oliveira S. , Mota M. 
Published: April 2009

Abstract
Until recently, the only entomopathogenic nematode (EPN) species reported from continental Portugal, was of the genus Steinernema. Following a national survey of EPNs in continental Portugal, several natural and managed habitats have been surveyed in the southern part of the country. From 57 soil samples collected using the Galleria mellonella trapping method, three samples yielded EPN. Morphological characterization and sequence analysis of the ITS regions of ribosomal DNA allowed the identification of EPN isolates as Heterorhabditis bacteriophora, representing the first report of this genus for continental Portugal.
Strategies in Prevention of Preharvest Aflatoxin Contamination in Peanuts: Aflatoxin Biosynthesis, Genetics and Genomics
Authors: Guo Baozhu , Yu Jiujiang , Holbrook C. Corley , Cleveland Thomas E. , Nierman William C. , Scully Brian T. 
Published: April 2009

Abstract
Peanut (Arachis hypogaea L.), or groundnut, is an important crop economically and nutritionally in many tropical and subtropical areas of the world. It is also one of the most susceptible host crops to Aspergillus flavus resulting in aflatoxin contamination. The prevention or elimination of aflatoxin contamination in preharvest and postharvest crops is a serious challenge facing scientists. The recent International Conference on Groundnut Aflatoxin Management and Genomics held in Guangzhou, China, provided an international forum for discussions on the latest accomplishments, the development of strategies, and the initiation of cooperative research for the prevention of aflatoxin contamination. This review summarizes the progress in genetic and genomic research of peanuts and the toxin-producing fungus A. flavus. In particular, the pathway for production and the genetic regulation of afaltoxin, and the peanut-Aspergillus interaction are discussed. The use of a peanut-Aspergillus microarray will help scientists to study the crop-pathogen interaction; aids in the identification of genes involved in both fungal invasion and crop resistance, and ultimately enhance research to find solutions that prevent aflatoxin contamination in agricultural commodities.
Review of Immunological Methods for the Quantification of Aflatoxins in Peanut and Other Foods
Authors: Waliyar Farid , Reddy S. V. , Lava-Kumar P. 
Published: April 2009

Abstract
Aflatoxin contamination is widespread in staple crops like peanut, maize, sorghum, pearl millet, chillies, pistachio, cassava etc., and compromises the safety of food and feed supplies. It is important to be able to detect and quantify aflatoxins in commodities to protect human and animal health. Many different methods, including antibody-based ones, are available for quantitative estimation of aflatoxins. However, most of these methods such as HPLC, HPTLC, and TLC are expensive and/or difficult to use in developed countries. Using the state-of-the-art facilities at ICRISAT, we developed polyclonal and monoclonal antibodies for the detection of total aflatoxins, aflatoxin B1 and M1 (secreted in milk). These were used to develop a simple and inexpensive competitive enzyme-linked immunosorbent assays (cELISA) that has lower detection limits (1.0 µg/kg) and cost (about $1 per sample) less than other available methods. More than 100 samples can be analyzed in a day. These tests have provided a unique opportunity for ICRISAT and its partners to conduct field studies to select resistant genotypes, identify high risk populations and determine the dietary sources to stimulate appropriate interventions to enhance the food and human health safety, trade and thereby farmers' income.
The U.S. Breeding Program to Develop Peanut with Drought Tolerance and Reduced Aflatoxin Contamination
Authors: Holbrook C. Corley , Guo B. Z. , Wilson D. M. , Timper P. 
Published: April 2009

Abstract
Aflatoxin contamination costs the U.S. peanut (Arachis hypogaea L.) industry over $20 million annually. The development of peanut cultivars with resistance to preharvest aflatoxin contamination (PAC) would reduce these costs. Screening techniques have been developed that can measure genetic differences in aflatoxin contamination and they have been used to identify accessions that exhibited relatively low PAC in multiple environments. Significant reductions in PAC have been identified in peanut genotypes with drought tolerance. These sources of resistance to PAC have been crossed with cultivars and breeding lines that have high yield, acceptable grade, and resistance to spotted wilt caused by Tomato spotted wilt tospovirus (TSWV). Due to the large environmental variation in PAC, breeding populations can only be evaluated in late generations when there is less heterozygosity and adequate numbers of seed are available for field testing using multiple replications. Evaluation of numerous breeding populations has identified several families and individual breeding lines with relatively low PAC, relatively high yield, and acceptable levels of resistance to TSWV. To increase breeding efficiency, studies on mechanisms of resistance to PAC are being conducted. The most promising mechanisms identified thus far are resistance to drought and resistance to the peanut root-knot nematode. Late generation breeding lines have been developed with resistance to drought, several of which also exhibited reduced aflatoxin contamination in multiple environments. Tifguard, the first cultivar with high levels of resistance to both TSWV and the peanut root-knot nematode [Meloidogyne arenaria (Neal) Chitwood race 1] was released from this program. Testing is ongoing to determine if this cultivar can be used to reduce aflatoxin contamination in nematode infested fields.
Influence of Digging Date and Fungicide Program on Canopy Defoliation and Pod Yield of Peanut (Arachis hypogaea L.)
Authors: Carley Danésha S. , Jordan David L. , Shew Barbara B. , Sutton Turner B. , Dharmasri L. Cecil , Brandenburg Rick L. 
Published: April 2009

Abstract
Although response can be variable and is dependant upon many factors, digging peanut as little as 1 wk prior to or 1 wk following optimum maturity can result in substantial reductions in pod yield. While growers often assume that harvest should be initiated prior to optimum maturity in fields with high levels of canopy defoliation, there is no clear threshold where gains in pod weight and grade are offset by losses due to pod shedding. Moreover, yield loss relationships for foliar diseases have not been characterized for modern virginia market-type cultivars. Determining interactions between digging date and peanut canopy defoliation would assist growers and their advisors in making more informed decisions on when to initiate harvest to avoid yield loss. Research conducted from 2004–2005 in North Carolina established a range of defoliation levels in a total of seven fields. Fungicides were applied on a bi-weekly schedule from early July through mid-September, or were applied two times in July, or were not applied. Plots were dug early, 6 to 12 days before estimated optimum maturity, or at optimum maturity, as determined by the hull-scrape method. Although interactions between digging date and fungicide program were found in some cases, digging date and fungicide program effects often varied independently. A general recommendation of the percentage of canopy defoliation justifying early digging to prevent yield loss could not be determined from this research. However, data from these experiments reinforce the value of controlling early leaf spot, late leaf spot, and web blotch with timely fungicide applications and the importance of digging at optimum pod maturation for more recently released virginia market-type peanut.
Influence of Application Variables on Peanut (Arachis hypogaea L.) Response to Prohexadione Calcium
Authors: Jordan David L. , Nuti Russell C. , Beam Joshua B. , Lancaster Sarah H. , Lanier James E. , Johnson P. Dewayne 
Published: April 2009

Abstract
Prohexadione calcium retards peanut vegetative vine growth, improves row visibility, and potentially reduces pod shed, thus increasing pod yield compared with non-treated peanut. Although prohexadione calcium has been registered for use in peanut for the past decade, practitioners continue to express a range of questions about use including banded application, compatibility with other agrichemcials, and interactions of application rate and timing. In experiments over multiple years, applying prohexadione calcium to lateral branches only of peanut increased row visibility compared with banded applications over main stems or broadcast applications over the entire peanut canopy. Similarly, when using different spray nozzle configurations, greater row visibility was noted when the highest rate of prohexadione calcium was applied over lateral branches compared with broadcast applications of a uniform rate across all spray nozzles or when the highest rate was delivered to main stems. Delaying the first of two sequential applications of prohexadione calcium 1 wk after 50% row closure resulted in reduced row visibility regardless of application rate when compared with sequential applications initiated at 50% row closure. Applying prohexadione calcium within 2 to 3 wks prior to digging and vine inversion resulted in minor increases in improved row visibility and did not affect pod yield. Efficacy of prohexadione calcium was not affected by tank mixing with pyraclostrobin or 2,4-DB.
Time course for autoregulation recovery following severe traumatic brain injury
Authors: Sviri Gill E. , Aaslid Rune , Douville Colleen M. , Moore Anne , Newell David W. 
Published: April 2009

Object
The aim of the present study was to evaluate the time course for cerebral autoregulation (AR) recovery following severe traumatic brain injury (TBI)

Methods
Thirty-six patients (27 males and 9 females, mean ± SEM age 33 ± 15.1 years) with severe TBI underwent serial dynamic AR studies with leg cuff deflation as a stimulus, until recovery of the AR responses was measured.

Results
The AR was impaired (AR index < 2.8) in 30 (83%) of 36 patients on Days 3–5 after injury, and in 19 individuals (53%) impairments were found on Days 9–11 after the injury. Nine (25%) of 36 patients exhibited a poor AR response (AR index < 1) on postinjury Days 12–14, which eventually recovered on Days 15–23. Fifty-eight percent of the patients with a Glasgow Coma Scale score of 3–5, 50% of those with diffuse brain injury, 54% of those with elevated intracranial pressure, and 40% of those with poor outcome had no AR recovery in the first 11 days after injury.

Conclusions
Autoregulation recovery after severe TBI can be delayed, and failure to recover during the 2nd week after injury occurs mainly in patients with a lower Glasgow Coma Scale score, diffuse brain injury, elevated ICP, or unfavorable outcome. The finding suggests that perfusion pressure management should be considered in some of the patients for a period of at least 2 weeks.
Use of the h index in neurosurgery
Authors: Lee Janet , Kraus Kristin L. , Couldwell William T. 
Published: April 2009

Object
Assessing academic productivity through simple quantification may overlook key information, and the use of statistical enumeration of academic output is growing. The h index, which incorporates both the total number of publications and the citations of those publications, has been recently proposed as an objective measure of academic productivity. The authors used several tools to calculate the h index for academic neurosurgeons to provide a basis for evaluating publishing by physicians.

Methods
The h index of randomly selected academic neurosurgeons from a sample of one-third of the academic programs in the US was calculated using data from Google Scholar and from the Scopus database. The mean h index for each academic rank was determined. The h indices were also correlated with various other factors (such as time spent practicing neurosurgery, authorship position) to identify how these factors influenced the h index. The h indices were then compared with other citation statistics to evaluate the robustness of this metric. Finally, h indices were also calculated for a sampling of physicians in other medical specialties for comparison.

Results
As expected, the h index increased with academic rank and there was a statistically significant difference between each rank. A weighting based on position of authorship did not affect h indices. The h index was positively correlated with time since American Board of Neurological Surgery certification, and it was also correlated with other citation metrics. A comparison among medical specialties supports the assertion that h index values may not be comparable between fields, even closely related specialties.

Conclusions
The h index appears to be a robust statistic for comparing academic output of neurosurgeons. Within the field of academic neurosurgery, clear differences of h indices between academic ranks exist. On average, an increase of the h index by 5 appears to correspond to the next highest academic rank, with the exception of chairperson. The h index can be used as a tool, along with other evaluations, to evaluate an individual's productivity in the academic advancement process within the field of neurosurgery but should not be used for comparisons across medical specialties.
Effect of WT1 gene silencing on the tumorigenicity of human glioblastoma multiforme cells
Authors: Clark Aaron J. , Ware Joy L. , Chen Mike Y. , Graf Martin R. , Van Meter Timothy E. , Dos Santos Wagner G. , Fillmore Helen L. , Broaddus William C. 
Published: April 2009

Object
Wilms tumor 1 (WT1) is overexpressed in many human cancers, including glioblastoma multiforme (GBM). In another study, the authors showed that transient WT1 silencing increases the radiosensitivity of glioma cells. Studies of nonglioma cell lines have demonstrated that WT1 promotes cell proliferation and survival; however, this ability has not been rigorously analyzed in human GBM.

Methods
The authors tested the efficacy of 2 sequences of short hairpin RNA (shRNA) directed against WT1 in U251MG human GBM cells and found that 1 sequence was capable of stably silencing WT1 expression. They then evaluated the effect of WT1 silencing on cellular proliferation, invasion, and in vivo tumor formation.

Results
Stable WT1-shRNA expression significantly decreased the proliferation of U251MG cells in vitro as demonstrated by both an adenosine 5′-triphosphate–based viability assay and tritiated thymidine uptake. Furthermore, stable WT1 silencing caused significantly slower growth after the subcutaneous inoculation of tumor cells in the flanks of athymic nude mice and was associated with an increased latency period.

Conclusions
Data in this study provide proof of the principle that downregulation of WT1 causes decreased tumorigenicity of a GBM cell line in vitro and in vivo and suggest that WT1 is a promising target for novel molecular GBM therapies, perhaps in combination with standard treatment modalities.