bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cytomorphological spectrum of breast fine needle aspiration cytology using the International Academy of Cytology Yokohama System and evaluation of associated risk of malignancy–A retrospective study in a tertiary care cente
Authors: Yadav Ranjan , Singh Nikita , Bakna Minu , Bhadkariya Anand , Singh Arjun 
Published: May 2024

Abstract
Background
Fine-needle aspiration cytology (FNAC) of the breast is a well-established technique that aids in the preoperative identification and differentiation between nonneoplastic and neoplastic breast lesions. Breast lesions can be efficiently categorized into different cytological groups using the International Academy of Cytology (IAC) Yokohama System. The aim of this study was to classify breast lesions based on the proposed Yokohama system of reporting breast cytology and identify the need for a unified system of reporting breast lesions.

Materials and methods
This retrospective descriptive study was performed in the Department of Pathology, Government Medical College, Datia, M.P. from January 2019 to June 2023. A total of 762 FNAC slides reported during this period were retrieved, re-examined and reclassified according to the IAC Yokohama System. Histopathological diagnoses were also retrieved and correlated with the cytological findings wherever available.

Results
In the present study, a total of 762 smears of breast fine needle aspirations were included and categorized using the IAC Yokohama System. There were 3.8% cases (29/762) in the C1 category (insufficient), 71.6% cases (545/762) in the C2 category (benign), 6.3% cases (48/762) in the C3 category (atypical), 3.1% cases (24/762) in the C4 category (suspicious for malignancy) and 15.2% (116/762) cases in the C5 category (malignant).

Conclusion
FNAC is a reliable diagnostic technique for the evaluation and management of breast lesions prior to surgery. Implementing the IAC Yokohama System for reporting breast cytopathology enhances the accuracy of diagnosing breast lesions, reduces missed diagnoses, and offers reliable estimates of risk of malignancy. Adopting the Yokohama system is a crucial first step in boosting the overall effectiveness of breast cytology reporting among clinicians and pathologists, which will ultimately lead to improved communication and patient care.
Systematic approach to contextualize findings of flexible endoscopic evaluation of swallowing in neurogenic dysphagia– towards an integrated FEES report
Authors: Dziewas Rainer , Warnecke Tobias , Labeit Bendix , Claus Inga , Muhle Paul , Oelenberg Stephan , Ahring Sigrid , Wüller Christina , Jung Anne , von Itter Jonas , Suntrup-Krueger Sonja 
Published: May 2024

AbstractFlexible endoscopic evaluation of swallowing (FEES) is one of the most important methods for instrumental swallowing evaluation. The most challenging part of the examination consists in the interpretation of the various observations encountered during endoscopy and in the deduction of clinical consequences. This review proposes the framework for an integrated FEES-report that systematically moves from salient findings of FEES to more advanced domains such as dysphagia severity, phenotypes of swallowing impairment and pathomechanisms. Validated scales and scores are used to enhance the diagnostic yield. In the concluding part of the report, FEES-findings are put into the perspective of the clinical context. The potential etiology of dysphagia and conceivable differential diagnoses are considered, further diagnostic steps are proposed, treatment options are evaluated, and a timeframe for re-assessment is suggested. This framework is designed to be adaptable and open to continuous evolution. Additional items, such as novel FEES protocols, pathophysiological observations, advancements in disease-related knowledge, and new treatment options, can be easily incorporated. Moreover, there is potential for customizing this approach to report on FEES in structural dysphagia.
A social media adoption strategy for cultural dissemination in municipalities with tourist potential: Lamas, Peru, as a case study
Authors: Vidaurre-Rojas Pierre , Vela-Reátegui Seidy Janice , Pinedo Lloy , Valles-Coral Miguel , Navarro-Cabrera Jorge Raul , Rengifo-Hidalgo Very , López-Sánchez Teresa del Pilar , Seijas-Díaz José , Cárdenas-García Ángel , Cueto-Orbe Rosa Elena 
Published: May 2024

AbstractSocial media is commonly used by governments to disseminate information related to transparency and institutional promotion, but its potential for the dissemination of cultural resources is often overlooked. This research proposes a strategy for adopting social media to achieve cultural dissemination in the municipality of Lamas, Peru, which has tourism potential and has been awarded the “Best Tourism Villages” seal of the World Tourism Organisation. Methodologically, we propose a case study at a propositional exploratory level. We start by determining the current state of dissemination of tangible and intangible resources, as well as the practical use of social media, through a survey of 20 officials. The results reveal that crafts are the most widely disseminated tangible resource in the municipality (according to 55% of officials), and intangible resources are occasionally disseminated (50%). We also find that Facebook is the only digital channel used to disseminate cultural information. In this context, we formulate a strategy for adopting social media to improve cultural dissemination, which consists of six phases: (i) forming a work team, (ii) preparing a budget plan, (iii) strengthening capacities, (iv) taking inventory of cultural resources, (v) coordinating community interests and (vi) preparing a cultural dissemination plan. The findings can contribute to formulating public initiatives for the institutionalisation of social media in governments for the purposes of preserving, promoting and guaranteeing the accessibility of cultural heritage.
Identification of Rare and Novel PHEX Variants in X-linked Hypophosphatemia
Authors: Ma Xiaosen , Pang Qianqian , Gong Yiyi , Li Xiang , Liu Wei , Jiang Yan , Wang Ou , Li Mei , Xing Xiaoping , Xia Weibo 
Published: May 2024

Abstract

Context
X-linked hypophosphatemia (XLH) is a rare metabolic bone disease caused by inactivation mutations in the PHEX gene. Despite the extensive number of reported PHEX variants, only a few cases of chromosomal abnormalities have been documented.

Objective
We aimed to identify the pathogenic variants in 6 unrelated families with a clinical diagnosis of XLH and to propose a genetic workflow for hypophosphatemia patients suspected of having XLH.

Methods
Multiple genetic testing assays were used to analyze the 6 families' genetic profiles, including whole exome sequencing, multiplex ligation-dependent probe amplification, whole genome sequencing, reverse transcript polymerase chain reaction, Sanger sequencing, and karyotyping.

Results
The study identified 6 novel pathogenic variants, including 1 mosaic variant (exon 16-22 deletion), 3 chromosomal abnormalities (46, XN, inv[X][pter→p22.11::q21.31→p22.11::q21.31 →qter], 46, XN, inv[X][p22.11p22.11], and XXY), a nonclassical intron variant (NM_000444.6, c.1701_31A &gt; G), and a deletion variant (NM_000444.6, c.64_5464-186 del5215) of PHEX. Additionally, a genetic testing workflow was proposed to aid in diagnosing patients suspected of XLH.

Conclusion
Our research expands the mutation spectrum of PHEX and highlights the significance of using multiple genetic testing methods to diagnose XLH.
Patient Characteristics, Management, and Outcomes in a Novel Cohort of Primary Hyperparathyroidism
Authors: Sant Vivek R , ElNakieb Yaser , Lehmann Christoph U , Rousseau Justin F , Maalouf Naim M 
Published: May 2024

Abstract

Context
Primary hyperparathyroidism (PHPT) increases the risk of bone loss, debilitating fractures, kidney stones, impaired renal function, and neurocognitive symptoms. Studies describing the natural history of PHPT have been limited to small samples, single institutions, or specific populations.

Objective
We assessed the natural history of PHPT through a large, diverse national cohort from an electronic health record dataset representing more than 100 million patients.

Methods
The TriNetX database was queried for adult patients with PHPT. We extracted demographics, comorbidities, and longitudinal biochemistries. Primary outcomes included major osteoporotic fracture (MOF) and chronic kidney disease (CKD). Outcomes were stratified by treatment strategy (surgical parathyroidectomy [PTX] vs nonsurgical) and age.

Results
Among 50 958 patients with PHPT, 26.5% were treated surgically at a median of 0.3 years postdiagnosis. At diagnosis, median age was 65 years, 74.0% were female, and median calcium level was 10.9 mg/dL. Black and older patients underwent PTX less frequently than White and younger patients. MOF 10-year incidence was 5.20% (PTX) and 7.91% (nonsurgical), with median 1.7-year delay with PTX compared to nonsurgical. PTX-associated MOF absolute risk reduction was 0.83% (age &lt; 65 years) and 3.33% (age ≥ 65 years). CKD 10-year incidence was 21.2% (PTX) and 33.6% (nonsurgical), with median 1.9-year delay with PTX. PTX-associated CKD absolute risk reduction was 12.2% (age &lt; 65 years) and 9.5% (age ≥ 65 years).

Conclusion
We report 1 of the largest, representative, population-based natural histories of PHPT with different management strategies. A minority of patients underwent PTX, especially in older age. Patients managed surgically had lower incidence of fracture and CKD, and older patients experienced differential benefit.
Functional exploration of copy number alterations in a Drosophila model of triple-negative breast cancer
Authors: Diaz Jennifer E. L. , Barcessat Vanessa , Bahamon Christian , Hecht Chana , Das Tirtha K. , Cagan Ross L. 
Published: May 2024

ABSTRACT
Accounting for 10-20% of breast cancer cases, triple-negative breast cancer (TNBC) is associated with a disproportionate number of breast cancer deaths. One challenge in studying TNBC is its genomic profile: with the exception of TP53 loss, most breast cancer tumors are characterized by a high number of copy number alterations (CNAs), making modeling the disease in whole animals challenging. We computationally analyzed 186 CNA regions previously identified in breast cancer tumors to rank genes within each region by likelihood of acting as a tumor driver. We then used a Drosophila p53-Myc TNBC model to identify 48 genes as functional drivers. To demonstrate the utility of this functional database, we established six 3-hit models; altering candidate genes led to increased aspects of transformation as well as resistance to the chemotherapeutic drug fluorouracil. Our work provides a functional database of CNA-associated TNBC drivers, and a template for an integrated computational/whole-animal approach to identify functional drivers of transformation and drug resistance within CNAs in other tumor types.
An ALS-associated mutation dysregulates microglia-derived extracellular microRNAs in a sex-specific manner
Authors: Christoforidou Eleni , Moody Libby , Joilin Greig , Simoes Fabio A. , Gordon David , Talbot Kevin , Hafezparast Majid 
Published: May 2024

ABSTRACT
Evidence suggests the presence of microglial activation and microRNA (miRNA) dysregulation in amyotrophic lateral sclerosis (ALS), the most common form of adult motor neuron disease. However, few studies have investigated whether the miRNA dysregulation originates from microglia. Furthermore, TDP-43 (encoded by TARDBP), involved in miRNA biogenesis, aggregates in tissues of ∼98% of ALS cases. Thus, this study aimed to determine whether expression of the ALS-linked TDP-43M337V mutation in a transgenic mouse model dysregulates microglia-derived miRNAs. RNA sequencing identified several dysregulated miRNAs released by transgenic microglia and a differential miRNA release by lipopolysaccharide-stimulated microglia, which was more pronounced in cells from female mice. We validated the downregulation of three candidate miRNAs, namely, miR-16-5p, miR-99a-5p and miR-191-5p, by reverse transcription quantitative polymerase chain reaction (RT-qPCR) and identified their predicted targets, which primarily include genes involved in neuronal development and function. These results suggest that altered TDP-43 function leads to changes in the miRNA population released by microglia, which may in turn be a source of the miRNA dysregulation observed in the disease. This has important implications for the role of neuroinflammation in ALS pathology and could provide potential therapeutic targets.
Basic life support training: Is student confidence enhanced by advanced levels of simulation?
Authors: Rushton Melanie , Pilkington Rachel 
Published: May 2024

Background: Basic life support (BLS) is a mandatory skill for nurses. The confidence of the BLS provider should be enhanced by regular training. Traditionally, BLS training has used low-fidelity manikins, but more recent studies have suggested the use of high-fidelity manikins and alternative levels of simulation such as virtual reality. Methods: A quasi-experimental study including 125 nursing students. Data on confidence levels in various elements of BLS were collected using pre-validated questionnaires and analysed using SPSSv23. Results: The study revealed that high-fidelity simulation had a significant impact on the BLS learner's confidence levels. Conclusion: The study identified the importance of high-fidelity simulation in BLS training in preparing students for clinical practice. This highlights the need for further exploration of simulation technologies, such as virtual reality, to enable students to gain the knowledge, skills, confidence and competence required to enable safe and effective practice.
ACUPUNTURA NO CUIDADO DO TRABALHADOR DA SAÚDE DURANTE A PANDEMIA POR COVID 19 : uma revisão integrativa.
Authors: Angieuski Camacho Beatriz , Aparecida Patricio Pereira Suellen , Manoel Oliveira Costa Lucas , Vieira da Silva Blamires Daniele , Aparecida de Sousa Moura Maria , Oliveira Melo Júlia 
Published: May 2024

Introdução: Durante a pandemia por Covid 19, práticas alternativas e complementares como a acupuntura foram utilizados no cuidado do trabalhador de saúde da linha de frente, com o objetivo de promover alívio às pressões e aos desafios enfrentados. Objetivo: Este estudo objetivou analisar na literatura as evidências científicas disponíveis acerca da utilização da acupuntura no cuidado do trabalhador da saúde durante a pandemia de Covid 19. Métodos: Foi feita uma revisão integrativa de literatura a partir de artigos da base de dados da Biblioteca Virtual em Saúde (BVS). A estratégia de busca para os artigos em português foi: (Acupuntura) AND (Covid 19) AND (Saúde do trabalhador) AND (Pessoal de Saúde). Para as buscas em inglês, utilizou-se a seguinte estratégia: (Acupuncture) AND (Covid 19) AND (Health Personnel) AND (Occupational Health). Foram selecionados os artigos primários, disponíveis na íntegra, publicados entre 2020 e 2022 em português ou inglês. Foram excluídos os que não respondessem à questão norteadora e estudos de revisão narrativa, tradicional, sistemática e integrativa. Resultados: A busca resultou em 59 artigos, 40 com a busca em inglês e 19 com a busca em português. Após aplicados os critérios de exclusão, foram selecionados 6 artigos para o estudo. Conclusão: Com a análise, concluiu-se que a utilização da acupuntura como recurso no cuidado aos profissionais de saúde, em especial durante a pandemia de Covid-19, quando utilizada, promoveu redução significativa nos níveis de estresse e ansiedade nos profissionais.
Propensity of Sawmills to Produce Structural Sawn Wood in Chile
Authors: Gysling Janina , Mejías Wilson , Herrera Miguel Ángel 
Published: May 2024

Abstract
The goals of Chile’s forest policy regarding the use of wood in construction are far from being achieved. Chile is the ninth-largest global producer of sawn softwood and has an industry with numerous sawmills; however, structural sawn wood (SSW) represents less than 1 percent of the industry’s total production, which is insufficient to address large-scale construction programs with wood. Therefore, policy instruments are needed to lead to an increase in the volume of SSW production. This research aimed to design a typology of sawmills that facilitates the definition and implementation of these policy instruments to ensure attainment of the 2035 goal of the forest policy. Multiple linear regression and multiple logistic regression were used to assess the impact of various variables on SSW production; variable selection was done using stepwise regression. The number of clusters was determined using the gap method, and sawmill clusters were determined using k means. The result is a typology with five categories of sawmills based on their propensity to produce SSW. The most significant variable was the quality of the supply, so it is suggested to prioritize the management of Pinus radiata plantations. With this result, a map for the application of policy instruments was created, proposing an intervention pathway. The proposed typology will stimulate decisions to provide direct support to the sawmill industry, thereby increasing SSW production.
Comparing the effectiveness of full-vacuum and half-vacuum drainage in reducing seroma after modified radical mastectomy: a meta-analysis
Authors: Oyewale S , Ariwoola A 
Published: May 2024

IntroductionOwing to limited outpatient support for drain management, delayed discharge after mastectomy is more prevalent in developing countries. Utilising half-vacuum (HV) suction drains for routine drainage after mastectomy could lead to a reduced incidence of seroma and a shorter hospital stay. This systematic review and meta-analysis compared the outcomes of HV against full-vacuum (FV) suction drains in patients who underwent modified radical mastectomy for breast cancer.MethodsDifferences between the two groups in length of hospital stay, total volume of drain effluent and incidence of seroma were assessed. RevMan 5.4 was used to calculate the odds ratio (OR) and relative risk (RR) for dichotomous data, and the mean difference (MD) for continuous data.ResultsNine randomised controlled trials were included in this review. Using HV drains reduced both the mean length of hospital stay (MD: −2.30 days, 95% confidence interval [CI]: −4.10 to −0.49 days, I2=97%) and the mean total volume of effluent (MD: −132.61ml, 95% CI: −207.32ml to −57.91ml, I2=88%) compared with FV drains. However, there was no statistically significant difference in incidence of seroma between the two groups (RR: 0.67, 95% CI: 0.30 to 1.46, I2=65%). Likewise, there was no difference in rate of seroma occurrence on sensitivity analysis (OR: 1.29, 95% CI: 0.72 to 2.33, I2=74%).ConclusionsThere was no difference in the incidence of seroma between HV and FV suction drainage. Conversely, a reduction in the length of hospital stay and the total volume of drain effluent was observed for mastectomy patients with a HV drain.
Living at work: migrant worker dormitories in Malaysia
Authors: Jones Katharine , Ghimire Anita , Khor Yvonne 
Published: May 2024

The COVID-19 pandemic in Malaysia drew attention to the extremely poor living conditions of the country’s approximately 2.5 million migrants from South and Southeast Asia working in manufacturing, construction, services, and agriculture. International media reports throughout 2020 and 2021 highlighted the overcrowded, unsanitary, and unsafe accommodations provided by employers, including cramped hostels, stacked containers, and rented apartments. This article addresses how migrant worker accommodation in Malaysia is utilised by the state and by employers as a spatial mechanism of control to regulate migrant labour.
This case study draws on over a hundred in-depth interviews with Nepali migrant workers, recruitment agents, employers, and policy officials in Malaysia. We detail how the Malaysian government’s requirement for migrants to live in employer-provided housing forms part of intensified immigration controls implemented by the federal government. This policy effectively transforms employers into ‘landlords’, bringing migrants’ ‘private space’ under their control, thereby enabling employers’ increased surveillance of their activities. We found that employers utilised the opportunity to discipline their workforces and intensify work regimes. We therefore argue that housing has become a double-layered regulatory tool to deepen labour control among migrant populations, perpetuate a state of temporariness, and reinforce visible boundaries between citizens and non-citizens. In the process, migrants’ living quarters (spaces of social reproduction) have been subsumed into the organisation of production, serving the demands of the low-wage, highly-controlled, political economy of Malaysia.
Identification of the needs and priorities of older people and stakeholders in rural and urban areas of Santo Andre, Brazil
Authors: Casadei Donatelli Danyela , Goodman-Palmer Dina , Odland Maria Lisa , Agyapong-Badu Sandra , da Cruz-Alves Natalia , Rosenburg Meire , Hirschhorn Lisa R. , Greig Carolyn , Davies Justine , Barbosa do Nascimento Vânia , Ferriolli Eduardo 
Published: May 2024

Background
There are few data reporting the needs and priorities of older adults in Brazil. This hampers the development and/or implementation of policies aimed at older adults to help them age well. The aim of this study was to understand areas of importance, priorities, enablers and obstacles to healthy ageing as identified by older adults and key stakeholders in both urban and rural environments.

Methods
Two locations were selected, one urban and one rural in the municipality of Santo André, in the metropolitan region of São Paulo (SP). Workshops for older adults (>60 y) and stakeholders were conducted separately in each location. The workshops incorporated an iterative process of discussion, prioritisation and ranking of responses, in roundtable groups and in plenary. Areas of commonality and differences between older adult and stakeholder responses were identified by comparing responses between groups as well as mapping obstacles and enablers to healthy ageing identified by older adults, to the priorities identified by stakeholder groups. The socio-ecologic model was used to categorise responses.

Results
There were few shared responses between stakeholders and older adults and little overlap between the top ranked responses of urban and rural groups. With respect to areas of importance, both stakeholder groups ranked policies for older people within their top five reponses. Both older adult groups ranked keeping physically and mentally active, and nurturing spirituality. There was a marked lack of congruence between older adults’ obstacles and enablers to healthy ageing and stakeholder priorities, in both urban and rural settings. Most responses were located within the Society domain of the socio-ecologic model, although older adults also responded within the Individual/ Relationships domains, particularly in ranking areas of most importance for healthy ageing.

Conclusions
Our results highlight substantial differences between older adults and stakeholders with respect to areas of importance, priorities, enablers and obstacles to healthy ageing, and point to the need for more engagement between those in advocacy and policymaking roles and the older people whose needs they serve.