bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epigenetic alterations of the SERPINE1 gene in oral squamous cell carcinomas and normal oral mucosa
Authors: Gao Shan , Nielsen Boye Schnack , Krogdahl Annelise , Sørensen Jens Ahm , Tagesen Jan , Dabelsteen Sally , Dabelsteen Erik , Andreasen Peter A. 
Published: March 2010

AbstractA high level of plasminogen activator inhibitor‐1 (PAI‐1 or SERPINE1) in tumor extracts is a marker of a poor prognosis in human cancers, including oral carcinomas. However, the mechanisms responsible for the upregulation of PAI‐1 in cancers remain unclear. Investigating specific PAI‐1 expressing cells in oral carcinomas by immunohistochemistry, we found that PAI‐1 was expressed in 18 of the 20 patients, mainly by cancer cells. Two showed PAI‐1 positive stromal cells surrounding the tumor areas and five showed PAI‐1 positive cells in tumor‐adjacent normal epithelium. By real‐time RT‐PCR analysis, 17 of 20 patients with oral carcinoma were found to have between 2.5‐ and 50‐fold increased tumor PAI‐1 mRNA level, as compared with the matched tumor‐adjacent normal tissues. The PAI‐1 mRNA level in connective tissues from 15 healthy volunteers was similar to the level in tumor‐adjacent normal tissues, but the level in epithelium was 5‐ to 10‐fold lower. Analyzing DNA methylation of 25 CpG sites within 960 bp around the transcription initiation site of the SERPINE1 gene by bisulfite sequencing, we did the surprising observation that both tumors and tumor‐adjacent normal tissue had a significant level of methylation, whereas there was very little methylation in tissue from healthy volunteers, suggesting that tumor‐adjacent normal tissue already contains transformation‐associated epigenetic changes. However, there was no general inverse correlation between PAI‐1 mRNA levels and SERPINE1 gene methylation in all tissues, showing that CpG methylation is not the main determinant of the PAI‐1 expression level in oral tissue. © 2010 Wiley‐Liss, Inc.
Comprehensive genetic analysis of seven large families with mismatch repair proficient colorectal cancer
Authors: Middeldorp Anneke , Jagmohan‐Changur Shantie C. , van der Klift Heleen M. , van Puijenbroek Marjo , Houwing‐Duistermaat Jeanine J. , Webb Emily , Houlston Richard , Tops Carli , Vasen Hans F. A. , Devilee Peter , Morreau Hans , van Wezel Tom , Wijnen Juul 
Published: March 2010

AbstractApproximately 40% of colorectal cancer (CRC) families with a diagnosis of hereditary nonpolyposis CRC on the basis of clinical criteria are not a consequence of mismatch repair (MMR) deficiency. Such families provide supporting evidence for the existence of a hitherto unidentified highly penetrant gene mutation. To gain further understanding of MMR‐competent familial colorectal cancer (FCC), we studied seven large families with an unexplained predisposition for CRC to identify genetic regions that could harbor CRC risk factors. First, we conducted a genome‐wide linkage scan using 10K single‐nucleotide polymorphism (SNP) arrays to search for disease loci. Second, we studied the genomic profiles of the tumors of affected family members to identify commonly altered genomic regions likely to harbor tumor suppressor genes. Finally, we studied the possible role of recently identified low‐risk variants in the familial aggregation of CRC in these families. Linkage analysis did not reveal clear regions of linkage to CRC. However, our results provide support linkage to 3q, a region that has previously been linked to CRC susceptibility. Tumor profiling did not reveal any genomic regions commonly targeted in the tumors studied here. Overall, the genomic profiles of the tumors show some resemblance to sporadic CRC, but additional aberrations were also present. Furthermore, the FCC families did not appear to have an enrichment of low‐risk CRC susceptibility loci. These data suggest that factors other than a highly penetrant risk factor, such as low or moderate‐penetrance risk factors, may explain the increased cancer risk in a subset of familial CRCs. © 2010 Wiley‐Liss, Inc.
Predicting and memorizing observed action: Differential premotor cortex involvement
Authors: Stadler Waltraud , Schubotz Ricarda I. , von Cramon D. Yves , Springer Anne , Graf Markus , Prinz Wolfgang 
Published: March 2010

AbstractMany studies have shown the involvement of the premotor cortex in action observation, recognizing this region as the neural marker of action simulation (i.e., internal modeling on the basis of the observer's own motor repertoire). So far, however, we have remained unaware of how action simulation differs from more general action representation in terms of premotor activation. The present fMRI experiment is the first to demonstrate how premotor structures contribute to action simulation as opposed to other action‐related cognitive tasks, such as maintaining action representations. Using similar stimuli, a prediction condition requiring internal simulation of transiently occluded actions was compared to three different action‐related control tasks differing solely in task instructions. Results showed right pre‐SMA activation as a correlate of maintaining action representations in general. Moreover, the prediction condition was most efficient in activating the left pre‐SMA and left PMd. These results suggest that the conjoint activation of the pre‐SMA and PMd reflects a core neural driver of action simulation. Hum Brain Mapp, 2011. © 2010 Wiley‐Liss, Inc.
Differential effects between cyclooxygenase‐2 inhibitors and siRNA on vascular endothelial growth factor production in head and neck squamous cell carcinoma cell lines
Authors: Park Seok‐Woo , Kim Hyo‐Sun , Hah Jeong‐Hun , Kim Kwang‐Hyun , Heo Dae‐Seog , Sung Myung‐Whun 
Published: March 2010

AbstractBackground:Several researchers have observed that cyclooxygenase‐2 (COX‐2) inhibitors display anticancer effects only at higher concentrations than doses that block COX‐2 activity in head and neck squamous cell carcinoma (HNSCC) cells.Methods:To better understand the exact anticancer mechanism of COX‐2‐inhibitors, we compared the effects of pharmacologic inhibitors to those of small‐interfering RNA against COX‐2 on cell‐growth, vascular endothelial growth factor (VEGF) production, and intracellular signaling in HNSCC cell lines.Results:We observed in HNSCC cells, that COX‐2‐siRNA induced an inhibitory effect on intracellular signaling, but unlike the pharmacologic inhibitors, did not affect cell proliferation. Whereas the chemical inhibitors increased VEGF synthesis even at low doses, COX‐2‐siRNA showed differential inhibition of VEGF production according to expression patterns of COX‐1 and COX‐2 in tested cells.Conclusion:The majority of the anticancer effects of COX‐2‐inhibitors in HNSCC cells seem to result from COX‐2‐independent action, suggesting that COX‐1 and COX‐2 may contribute to VEGF synthesis in cancer cells through a prostaglandin‐dependent mechanism. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Head and neck cancer: Response to p53‐based therapeutics
Authors: Nemunaitis Jackie , Nemunaitis John 
Published: March 2010

AbstractLimited options are available for patients with advanced stage head and neck cancer. The p53 gene is known as the “guardian of the genome.” Mutations of the p53 gene predispose to carcinogenesis. The p53 mutations are common in head and neck cancer. Replacement of p53 gene function in preclinical models demonstrates cancer regression and improved survival. Clinical data with an adenoviral based p53 gene delivery product (Advexin) supports safety and clinical response after direct intratumoral injection. We summarize p53‐related therapeutics in this review. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Pharmacodynamic evaluation of temsirolimus in patients with newly diagnosed advanced‐stage head and neck squamous cell carcinoma
Authors: Ekshyyan Oleksandr , Mills Glenn M. , Lian Timothy , Amirghahari Nazanin , Rong Xiaohua , Lowery‐Nordberg Mary , Abreo Fleurette , Veillon Diana M. , Caldito Gloria , Speicher Lisa , Glass Jonathan , Nathan Cherie‐Ann O. 
Published: March 2010

AbstractBackground:Activation of the mammalian target of rapamycin (mTOR) pathway in surgical margins of head and neck squamous cell carcinoma (HNSCC) is a predictor of recurrence and patients with minimal residual disease may benefit from adjuvant therapy with temsirolimus, an mTOR inhibitor.Methods:The effects of 3 weekly doses of 25 mg of temsirolimus on Akt/mTOR pathway biomarkers were evaluated in tumor and peripheral blood mononuclear cells (PBMCs) of patients with HNSCC. Adverse events were assessed.Results:Temsirolimus significantly decreased pS6 and p4E‐BP1 in tumors, and pS6 and pAkt in PBMCs (p < .05). There was no significant upregulation of pAkt(Ser473) in tumor tissue. Side effects were minimal and reversible.Conclusion:Significant inhibition of the mTOR pathway was noted in both tumors and PBMCs of HNSCC with minimal side effects. The mTOR inhibitors can potentially be used as adjuvant therapy for patients with minimal residual disease and PBMCs are potential surrogate markers in this setting. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Three New Diterpenoids from Rabdosia lophanthoides var. gerardiana
Authors: Li Chen‐Yang , Gao Hao , Jiao Wei‐Hua , Zhang Long , Zhou Guang‐Xiong , Yao Xin‐Sheng 
Published: March 2010

AbstractThree new diterpenoids, together with three known ones, were isolated from the air‐dried whole herbs of Rabdosia lophanthoides var. gerardiana. The structures of the new diterpenoids were established as 3,4‐dihydro‐11‐hydroxy‐10‐(1‐hydroxy‐1‐methylethyl)‐2,2,6‐trimethylnaphtho[1,8‐bc]oxocin‐5(2H)‐one (1), 11,12,15‐trihydroxyabieta‐5,8,11,13‐tetraen‐7‐one (2), (2R,3S,4S,4aR,8S,9aS,13aS,16aS)‐3,4,4a,8,9,9a,10,11,12,13,14,16a‐dodecahydro‐2‐(hydroxymethyl)‐6,6,10,10‐tetramethyl‐2H‐benzo[4,5]cyclohepta[1,2‐h]pyrano[2,3‐b][1,4]benzodioxepine‐3,4,8,13a,15(6H)‐pentol (3) by spectroscopic methods, including extensive 1D‐ and 2D‐NMR analyses. The structures of the known compounds were identified by comparison of their physical and spectroscopic data with those reported in the literature.
Synthesis of Novel Spiropyrimidinones
Authors: Amutha Parasuraman , Nagarajan Samuthirapandian 
Published: March 2010

AbstractAn efficient one‐pot synthesis of new spirocyclic 2‐aminopyrimidinones via the three‐component reaction of a cyclic ketone, alkyl cyanoacetate, and guanidinium carbonate was developed via a domino reaction.
One‐Pot, Three‐Component Synthesis of 1‐(2‐Hydroxyethyl)‐1H‐1,2,3‐triazole Derivatives by Copper‐Catalyzed 1,3‐Dipolar Cycloaddition of 2‐Azido Alcohols and Terminal Alkynes under Mild Conditions in Water
Authors: Sharghi Hashem , Hosseini‐Sarvari Mona , Moeini Fatemeh , Khalifeh Reza , Beni Alireza Salimi 
Published: March 2010

AbstractA safe, efficient, and improved procedure for the regioselective synthesis of 1‐(2‐hydroxyethyl)‐1H‐1,2,3‐triazole derivatives under ambient conditions is described. Terminal alkynes reacted with oxiranes and NaN3 in the presence of a copper(I) catalyst, which is prepared by in situ reduction of the copper(II) complex 4 with ascorbic acid, in H2O. The regioselective reactions exclusively gave the corresponding 1,4‐disubstituted 1H‐1,2,3‐triazoles in good to excellent yields. This procedure avoids the handling of organic azides as they are generated in situ, making this already powerful click process even more user‐friendly and safe. The remarkable features of this protocol are high yields, very short reaction times, a cleaner reaction profile in an environmentally benign solvent (H2O), its straightforwardness, and the use of nontoxic catalysts. Furthermore, the catalyst could be recovered and recycled by simple filtration of the reaction mixture and reused for ten consecutive trials without significant loss of catalytic activity. No metal‐complex leaching was observed after the consecutive catalytic reactions.
Parathyroid hormone (PTH) peptides through the regulation of hyaluronan metabolism affect osteosarcoma cell migration
Authors: Berdiaki Aikaterini , Datsis Georgios A. , Nikitovic Dragana , Tsatsakis Aristidis , Katonis Pavlos , Karamanos Nikos K. , Tzanakakis George N. 
Published: March 2010

AbstractParathyroid hormone (PTH) strongly stimulates hyaluronan (HA) synthesis and secretion of both normal and carcinogenic cells of the osteoblastic lineage and improves skeletal microarchitecture. HA, a glycosaminoglycan component of the extracellular matrix (ECM), is capable of transmitting ECM‐derived signals to regulate cellular function. In this study, we investigated whether the changes of HA metabolism induced by PTH (1–34) and PTH (7–84) peptides in moderately MG‐63 and well‐differentiated Saos 2 osteosarcoma cell lines, are correlated to their migration capabilities. Our results demonstrate that intermittent PTH (1–34) treatment significantly (P ≤ 0.01) supported the migration of MG‐63 cells, increased their HA‐synthase‐2 (HAS2) expression (P ≤ 0.001), and enhanced their high‐molecular size HA deposition in the pericellular matrix. Both increased endogenous HA production (P ≤ 0.01) and treatment with exogenous high‐molecular weight HA (P ≤ 0.05) correlated to a significant increase of MG‐63 cell migration capacity. Transfection with siHAS2 showed that PTH (1–34), mainly through HAS2, enhanced HA and regulated MG‐63 cell motility. Interestingly, continuous PTH (1–34) treatment stimulated both Saos 2 cell HAS2 (P ≤ 0.001) and HAS1 (P ≤ 0.001) isoform expression inhibited their HYAL2 expression (P ≤ 0.001) and modestly (P ≤ 0.05) enhanced their migration. Therefore, the PTH (1–34) administration mode appears to distinctly modulate the migratory responses of the MG‐63 moderately and Saos 2 well‐differentiated osteosarcoma cell lines. Conclusively, the obtained data suggest that there is a regulatory effect of PTH (1–34), in an administration mode‐dependent manner, on HA metabolism that is essential for osteosarcoma cell migration. © 2010 IUBMB IUBMB Life, 62(5): 377–386, 2010
Comparison of broadband and ultrabroadband pulses at MHz and GHz pulse‐repetition rates for nonlinear femtosecond‐laser scanning microscopy
Authors: Studier Hauke , Breunig Hans Georg , König Karsten 
Published: March 2010

AbstractNonlinear optical imaging of human skin and of polychromatic microspheres was carried out to compare and evaluate the imaging properties of three different excitation femtosecond lasers: a spectrally tunable 80 MHz Ti: sapphire oscillator that produced 100 fs pulses (spectral width ∼10 nm) and two ultrabroadband Ti: sapphire oscillators with repetition rates of 85 MHz and 1 GHz. The latter of these two and the 100 fs laser were combined with a laser scanning microscope (TauMap). The intensities of images of the polychromatic microsphere samples obtained with both lasers are in accordance with the usual dependence of two‐photon processes on laser pulse parameters, i.e. the intensity is proportional to the square of the mean laser power and the reciprocal pulse duration. In contrast to that, skin images measured with all three different excitation sources with mean powers of each laser adjusted to the particular pulse length and repetition rate exhibited discrepancies from this relation. For characterization of the ultrabroadband GHz laser, the measurements are supplemented by spectra of second‐harmonic‐generation signals of urea and collagen. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Controlled application and removal of liposomal therapeutics: Effective elimination of pegylated liposomal doxorubicin by double‐filtration plasmapheresisin vitro
Authors: Pütz Gerhard , Schmah Oliver , Eckes Jürgen , Hug Martin J. , Winkler Karl 
Published: March 2010

AbstractIntroduction:Nanoscale particle‐based drug delivery systems like long circulating liposomal doxorubicin show unique pharmacokinetic properties and improved toxicity profiles. Liposomal doxorubicin accumulates in tumor tissue due to the enhanced permeation and retention effect, but only a small fraction of a total dose reaches the tumor site. Accumulation of liposomal doxorubicin is much faster in tumor sites than in certain organs where dose limiting adverse effects occur. Finding a way to detoxify the predominant part of a given dose, circulating in the blood after accumulation is completed, will presumably reduce severe side effects during chemotherapy.Methods:Elimination properties of therapeutic used pegylated liposomal doxorubicin (Doxil®/Caelyx®) and therapeutic used double‐filtration plasmapheresis systems were evaluatedin vitroand in reconstituted human blood.Results:Liposomes can be filtered by appropriate membranes without leakage of doxorubicin up to a pressure of 1 bar. At higher pressures, liposomes (∼85 nm) may squeeze through much smaller pores without significant leakage of doxorubicin, whereas decreasing pore size to ∼8 nm leads to increased leakage of doxorubicin. With therapeutic used apheresis systems, liposomal doxorubicin can be efficiently eliminated out of buffer medium and reconstituted human blood. No leakage of doxorubicin was detected, even when liposomes were circulating for 48 h in human plasma before apheresis.Conclusions:Convenient apheresis techniques are capable of a safe and efficient elimination of therapeutic used liposomal doxorubicin in an experimental model system. J. Clin. Apheresis, 2010. © 2010 Wiley‐Liss, Inc.
Practical modeling of molecular systems with symmetries
Authors: Grudinin Sergei , Redon Stephane 
Published: March 2010

AbstractA new method for efficient modeling of macromolecular systems with symmetries is presented. The method is based on a hierarchical representation of the molecular system and a novel fast binary tree‐based neighbor list construction algorithm. The method supports all types of molecular symmetry, including crystallographic symmetry. Testing the proposed neighbor list construction algorithm on a number of different macromolecular systems containing up to about 200,000 of atoms shows that (1) the current binary tree‐based neighbor list construction algorithm scales linearly in the number of atoms for the central subunit, and sublinearly for its replicas, (2) the overall computational overhead of the method for a system with symmetry with respect to the same system without symmetry scales linearly with the cutoff value and does not exceed 50% for all but one tested macromolecules at the cutoff distance of 12 Å. (3) the method may help produce optimized molecular structures that are much closer to experimentally determined structures when compared with the optimization without symmetry, (4) the method can be applied to models of macromolecules with still unknown detailed structure. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
Do anionic titanium dioxide nano‐clusters reach bulk band gap? A density functional theory study
Authors: Qu Zheng‐Wang , Zhu Hui 
Published: March 2010

AbstractThe electronic properties of both neutral and anionic (TiO2)n (n = 1–10) clusters are investigated by extensive density functional theory calculations. The predicted electron detachment energies and excitation gaps of anionic clusters agree well with the original experimental anion photoelectron spectra (APES). It is shown that the old way to analyze APES tends to overestimate vertical excitation gaps (VGA) of large anionic clusters, due to the nature of multiple electronic origins for the higher APES bands. Moreover, the VGA of anionic TiO2 clusters are evidently smaller than those of neutral clusters, which may also be the case for other metal oxide clusters with high electron affinity. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
Investigation of the molecular surface area and volume: Defined and calculated by the molecular face theory
Authors: Gong Li‐Dong , Yang Zhong‐Zhi 
Published: March 2010

AbstractBased on the molecular face (MF) theory, the molecular face surface area (MFSA) and molecular face volume (MFV) are defined. For a variety of organic molecules and several inorganic molecules, the MFSA and MFV have been studied and calculated in terms of an algorithm of our own via the Matlab package. The MFV shows a very good linear relationship with the experimentally measured critical molar volume. It is also found that the MFSA and MFV have significant linear correlations with those of the commonly used hard‐sphere model and the electron density isosurface. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
A NEMO potential that includes the dipole–quadrupole and quadrupole–quadrupole polarizability
Authors: Holt Asbjørn , Boström Jonas , Karlström Gunnar , Lindh Roland 
Published: March 2010

AbstractTo increase the accuracy of molecular force fields a systematical and balanced improvement of the various terms included is needed. In this work, we have followed this strategy to improve the quality of the NEMO potential for the formaldehyde dimer by introducing local quadrupole moments and higher‐order polarizabilities. It is found that inclusion of the quadrupole moment significantly improves the interaction potential. Furthermore, the inclusion of higher‐order polarizabilities up to quadrupole–quadrupole polarizability is shown to give a better description of the intermolecular interaction. In addition, it is demonstrated that localized properties based on MP2 densities reproduces the BSSE corrected MP2 interaction energy at large intermolecular separations. This is not the case for HF–SCF based properties. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
Psychotherapy with rural religious fundamentalist clients
Authors: Aten Jamie D. , Mangis Michael W. , Campbell Clark 
Published: March 2010

AbstractSuccessful psychotherapy with rural fundamentalist Christians requires psychologists to understand the clients' culture and worldview. They often rely heavily on religious authorities, interpret Scriptures literally, adhere to strict moral codes of behavior, and believe that they should evangelize those around them. Common therapeutic challenges include: spiritualizing problems, relational conflicts related to gender role expectations, addiction problems, and the religious agendas of family and clergy. We recommend that psychotherapists evaluate their own attitudes, collaborate with community gatekeepers, sensitively address clients' rigid beliefs, address religious differences, and take a holistic approach to treatment. A case example illustrates this approach. © 2010 Wiley Periodicals, Inc. J Clin Psychol: In Session 66:1–11, 2010.
Ethical challenges of practicing in rural areas
Authors: Werth James L. , Hastings Sarah L. , Riding‐Malon Ruth 
Published: March 2010

AbstractMental health professionals practicing in rural areas face ethical dilemmas different from those experienced by their urban counterparts and may find that the existing ethics literature and American Psychological Association (APA, 2002) ethics code not particularly helpful. We highlight parts of five standards from the APA ethics code to illustrate the dilemmas rural practitioners frequently confront and offer suggestions for how to handle them. We discuss competence, human relations, and confidentiality as specific areas and then examine assessment and therapy as broader situations in which dilemmas may occur. We use case examples to highlight complications that may arise in rural areas. © 2010 Wiley Periodicals, Inc. J Clin Psychol: In Session 66:1–12, 2010.