bluuebunny/crossref_metadata_2025_split huggingface dataset:
Determination of glyphosate and its metabolites in plant material by reversed‐polarity CE with indirect absorptiometric detection
Authors: Rojano‐Delgado Antonia María , Ruiz‐Jiménez José , de Castro María Dolores Luque , De Prado Rafael 
Published: March 2010

AbstractA simple CE method for simultaneous determination of glyphosate and its metabolites (i.e. aminomethylphosphonic acid, glyoxylate, sarcosine and formaldehyde) in plants is reported here. A BGE of pH 7.5, 10% ACN, 7.5 mM phthalate, containing 0.75 mM hexadecyltrimethylammonium bromide as an electro‐osmotic flow modifier, an applied voltage of –20 kV and absorptiometric monitoring at 220 nm were the optimal chemical and instrumental parameters. The method, with development time 20 min, shows linear calibrations within the range 5–500 μg/mL (for all target analytes) with correlation coefficients between 0.999 and 0.998. It has been validated by application to samples of Lolium spp. The electroinjection mode hinders most interferents to enter the capillary, thus providing a clean electropherogram and making unnecessary long sample‐preparation steps.
Enantioselective CE analysis of hepatic ketamine metabolism in different speciesin vitro
Authors: Schmitz Andrea , Thormann Wolfgang , Moessner Lone , Theurillat Regula , Helmja Kati , Mevissen Meike 
Published: March 2010

AbstractKetamine, an injectable anesthetic and analgesic consisting of a racemic mixture ofS‐andR‐ketamine, is routinely used in veterinary and human medicine. Nevertheless, metabolism and pharmacokinetics of ketamine have not been characterized sufficiently in most animal species. An enantioselective CE assay for ketamine and its metabolites in microsomal preparations is described. Racemic ketamine was incubated with pooled microsomes from humans, horses and dogs over a 3 h time interval with frequent sample collection. CE data revealed that ketamine is metabolized enantioselectively to norketamine (NK), dehydronorketamine and three hydroxylated NK metabolites in all three species. The metabolic patterns formed differ in production rates of the metabolites and in stereoselectivity of the hydroxylated NK metabolites.In vitropharmacokinetics of ketamineN‐demethylation were established by incubating ten different concentrations of racemic ketamine and the single enantiomers of ketamine for 8 min and data modeling was based on Michaelis–Menten kinetics. These data revealed a reduced intrinsic clearance of theS‐enantiomer in the racemic mixture compared with the singleS‐enantiomer in human microsomes, no difference in equine microsomes and the opposite effect in canine microsomes. The findings indicate species differences with possible relevance for the use of singleS‐ketamineversusracemic ketamine in the clinic.
Impact of land use on the hydraulic properties of the topsoil in a small French catchment
Authors: Gonzalez‐Sosa E. , Braud I. , Dehotin J. , Lassabatère L. , Angulo‐Jaramillo R. , Lagouy M. , Branger F. , Jacqueminet C. , Kermadi S. , Michel K. 
Published: March 2010

AbstractThe hydraulic properties of the topsoil control the partition of rainfall into infiltration and runoff at the soil surface. They must be characterized for distributed hydrological modelling. This study presents the results of a field campaign documenting topsoil hydraulic properties in a small French suburban catchment (7 km2) located near Lyon, France. Two types of infiltration tests were performed: single ring infiltration tests under positive head and tension‐disk infiltration using a mini‐disk. Both categories were processed using the BEST—Beerkan Estimation of Soil Transfer parameters—method to derive parameters describing the retention and hydraulic conductivity curves. Dry bulk density and particle size data were also sampled. Almost all the topsoils were found to belong to the sandy loam soil class. No significant differences in hydraulic properties were found in terms of pedologic units, but the results showed a high impact of land use on these properties. The lowest dry bulk density values were obtained in forested soils with the highest organic matter content. Permanent pasture soils showed intermediate values, whereas the highest values were encountered in cultivated lands. For saturated hydraulic conductivity, the highest values were found in broad‐leaved forests and small woods. The complementary use of tension‐disk and positive head infiltration tests highlighted a sharp increase of hydraulic conductivity between near saturation and saturated conditions, attributed to macroporosity effect. The ratio of median saturated hydraulic conductivity to median hydraulic conductivity at a pressure of − 20 mm of water was about 50. The study suggests that soil texture, such as used in most pedo‐transfer functions, might not be sufficient to properly map the variability of soil hydraulic properties. Land use information should be considered in the parameterizations of topsoil within hydrological models to better represent in situ conditions, as illustrated in the paper. Copyright © 2010 John Wiley & Sons, Ltd.
New diagnostic estimates of variations in terrestrial water storage based on ERA‐Interim data
Authors: Mueller Brigitte , Hirschi Martin , Seneviratne Sonia I. 
Published: March 2010

AbstractTerrestrial water storage is an important component of the climate system as it determines the partitioning of the water and energy fluxes at the land surface. Since in situ measurements are scarce, there is a strong need for alternative validation data sources.Here we present a new dataset of monthly basin‐scale terrestrial water storage changes (TWSC) derived with the combined atmospheric‐terrestrial water‐balance approach, also referred to as basin‐scale water‐balance (BSWB) approach. It is diagnosed from observation‐constrained reanalysis data for the atmospheric fields and observed streamflow. A previous BSWB dataset derived from the European Center for Medium‐Range Weather Forecasts (ECMWF) reanalysis ERA‐40 provided reasonable estimates of TWSC for mid‐latitude basins. The here presented BSWB dataset is derived from the latest ECMWF reanalysis ERA‐Interim, which is available in real time. The potential real‐time availability of TWSC derived from ERA‐Interim is of great relevance for several agricultural, hydrological, and climate applications.An uncertainty analysis of the derived TWSC highlights the importance of the atmospheric moisture flux convergence fields from the reanalysis for the resulting product uncertainty. We find that the BSWB estimates have a relative uncertainty of about 4–6% in basins larger than 1·5 × 106 km2 compared with the mean seasonal cycle. The ERA‐Interim based dataset compares better with in situ soil moisture, snow depth, and groundwater measurements in the Ob basin and Illinois than the previous ERA‐40 based dataset. We further compare the BSWB datasets to retrievals from the Gravity Recovery And Climate Experiment (GRACE), scatterometer data from the European Remote Sensing (ERS) satellites, and land surface model output from the second phase of the Global Soil Wetness Project (GSWP). We find a better correlation between ERS and the BSWB data than between GRACE and the BSWB data, which might be related to the relatively coarse resolution of GRACE. GSWP model output and the BSWB datasets compare well in most of the studied river basins. Copyright © 2010 John Wiley & Sons, Ltd.
Biochemical comparisons of osteoarthritic human synovial fluid with calf sera used in knee simulator wear testing
Authors: Brandt J.‐M. , Brière L. K. , Marr J. , MacDonald S. J. , Bourne R. B. , Medley J. B. 
Published: March 2010

AbstractOsteoarthritic human synovial fluid was obtained from the knees of 20 patients and was compared with four different calf sera solutions frequently used as lubricants in knee simulator wear testing. Assuming that the fluid after arthroplasty was the same as the fluid in patients with osteoarthritis, the total protein concentration, protein constituent fractions, osmolality, trace element concentrations, and the thermal stability obtained via differential scanning calorimetry were determined. Human synovial fluid, with an average total protein concentration of 34 g/L, was significantly different from all undiluted calf sera. However, alpha‐calf serum and iron‐supplemented alpha‐calf serum were closest in protein constituent fractions (albumin, α‐1‐globulin, α‐2‐globulin, ß‐globulin, and γ‐globulin) to human synovial fluid. Diluting calf sera with low‐ion distilled water to a total protein concentration of 17 g/L (as recommended by ISO 14243) produced non‐clinically relevant total protein concentration and osmolality levels. Performing the same dilution of iron‐supplemented alpha‐calf serum with phosphate‐buffered saline solution and 1.5 g/L hyaluronic acid produced an artificial lubricant with both a clinically relevant level of osmolality and clinically relevant thermal stability as seen in human synovial fluid from patients with osteoarthritis. The present study suggested that alpha‐calf serum, phosphate‐buffered saline solution and hyaluronic acid were essential constituents of an artificial lubricant to mimic the major biochemical properties of human synovial fluid for simulator wear testing of total knee replacements. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010
Tissue response to the components of a hydroxyapatite‐coated composite femoral implant
Authors: Hacking S. A. , Pauyo T. , Lim L. , Legoux J. G. , Bureau M. N. 
Published: March 2010

AbstractBone loss around femoral implants used for THA is a persistent clinical concern. It may be caused by stress shielding, generally attributed to a mismatch in stiffness between the implants and host bone. In this regard, a fatigue resistant, carbon fiber (CF) composite femoral implant with bone‐matching stiffness has been developed. This study evaluated the tissue response to the three material components of this implant in normal and textured (blasted with 24 grit alumina) surfaces: the hydroxyapatite (HA) coating, the CF composite and the intermediate crystalline HA particulate composite layer to bond to the HA coating (blended). Sprague‐Dawley rats underwent bilateral femoral implantation each receiving two rod‐like implants. Bone apposition to the HA (37%) and textured Ti (41%) implants was not significantly different. Bone apposition to the untextured CF (14%) and blended (19%) implants and polished Ti (8%) implants was significantly lower. Bone apposition to the textured CF (9%) and blended (11%) implants was lower (but not statistically from the as received or untextured counterparts). Nearly all sections from femurs containing CF implants presented CF debris. There was no evidence of localized bone loss or any strong immune response associated with any of the implant materials. All materials were well tolerated with minimal inflammation despite the presence of particulate debris. The high degree of bone apposition to the HA‐coated composite implants and the lack of short‐term inflammation and adverse tissue response to the three material implant component support continued evaluation of this composite technology for use in THA. Published 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010
The influence of proteins on demineralization kinetics of hydroxyapatite aggregates
Authors: Kosoric Jelena , Hector Mark P. , Anderson Paul 
Published: March 2010

AbstractSalivary proteins influence the biomineralization of hydroxyapatite (HAp) within enamel. Their effect on the crystal growth has been extensively studied, but, their effect on demineralization kinetics is less well investigated. In this study bovine serum albumin (BSA) was used as a model protein to measure its effect on demineralization kinetics of hydroxyapatite aggregates using scanning microradiography (SMR). HAp aggregates (8 and 20% porous) were cut into 5 × 5 × 2 mm blocks. SMR cells were prepared containing hydroxyapatite blocks. BSA was added to demineralising solutions (0.1 mol L−1 acetic acid, buffered to pH 4.0; degree of saturation zero and 0.062 respectively) at a concentration range 0.76–75.8 μmol L−1. Demineralising solution without added BSA was used as a control. The demineralising solutions were circulated past the samples at 0.4 mL min−1. SMR was used to measure the rate of mineral loss (RMLHAp) at 14 points in each sample repeatedly for 3 weeks. The results show that BSA increases or decreases the RMLHAp depending on; BSA concentration, HAp porosity, and the degree of saturation of the demineralising solution. It is suggested that BSA influences demineralization kinetics of HAp either by modifying solution properties, or, by affecting the surface energy of hydroxyapatite. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010
Greater endothelial cell responses on submicron and nanometer rough titanium surfaces
Authors: Lu Jing , Khang Dongwoo , Webster Thomas J. 
Published: March 2010

AbstractAn in vitro competitive coculture system was designed in this study to investigate vascular cell functions on submicron (lateral feature sizes larger than 100 nm) and nanometer (lateral and vertical feature sizes smaller than 50 nm) rough titanium surface features created via e‐beam evaporation with identical chemistry to that of control (unmodified) titanium. Rat aortic endothelial cells (RAEC) and rat aortic smooth muscle cells (RASMC) were individually cultured and cocultured on such substrates for 4 h as well as 1, 3, and 5 days. In addition, RAEC and RASMC intracellular collagen and elastin synthesis were determined for up to 14 days on the various substrates. Compared to control flat titanium surfaces, the results showed for the first time that submicron rough surface features on titanium enhanced RAEC proliferation (followed by nanometer surface features), while simultaneously inhibiting RASMC proliferation. In addition, protein synthesis assays demonstrated enhanced collagen and elastin production by RAEC cultured on submicron rough titanium substrates (followed by nano rough) compared to control flat titanium substrates. Surface characterization results showed that submicron rough featured titanium possessed the greatest hydrophilicity (followed by nanometer surface featured), which may have promoted select protein adsorption to increase RAEC over RASMC function. In contrast to RASMC, RAEC morphology was significantly altered by the underlying titanium surface features, which may also explain the altered vascular cell functions on the different substrates. Thus, this study suggested that submicron to nanometer rough surface features should be further studied for improving titanium for vascular stent applications. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Microbial adhesion to surface‐grafted polyacrylamide brushes after long‐term exposure to PBS and reconstituted freeze‐dried saliva
Authors: Fundeanu Irina , van der Mei Henny C. , Schouten Arend J. , Busscher Henk J. 
Published: March 2010

AbstractPolyacrylamide (PAAm) brushes, covalently grafted from silicon wafer surfaces were examined for their ability to inhibit microbial adhesion after long‐term exposure to PBS or reconstituted freeze‐dried saliva for time intervals from 48 h up to 1 month at 37°C. Microbial adhesion after exposure was studied in a parallel plate flow chamber. Infrared spectra showed that PAAm brushes exhibit good chemical stability upon incubation in both PBS and reconstituted freeze‐dried saliva up to 1 month. Reductions in microbial adhesion on PAAm brushes after exposure to PBS or reconstituted freeze‐dried saliva varied from 63 to 93% depending on the microbial strain considered, even after 1 month of exposure of the brushes to reconstituted freeze‐dried saliva. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
The study of specific and nonspecific hepatoma cells behavior by means of plasma‐treated substrates
Authors: Nardulli M. , Belviso M. , Favia P. , d'Agostino R. , Gristina R. 
Published: March 2010

AbstractPhysical‐chemical surface modifications represent a formidable tool to drive a suitable cell behavior on materials intended to be used in the biomedical field. Plasma processes are among the more powerful methods utilized to modify the surface of materials without altering their bulk intrinsic properties. In particular, by means of plasma treatment processes it is possible to graft chemical functional groups on polymer substrate. Functional groups grafted on the surface can improve per se cell adhesion and can also represent suitable anchor sites for biomolecule immobilization. The aim of this work was to determine the effect of plasma treatment and biomolecule immobilization on Polystyrene (PS) Petri dishes on the behavior of a human hepatocellular carcinoma cell line (HepG2). For this aim Petri dishes were grafted with N‐containing groups in order to obtain grafted N‐functionalities, to be used as anchor groups for the immobilization of galactosamine. In this way two different modified surfaces, NH3 grafted polystyrene (PS‐NH3) and polystyrene owing galactosamine moieties (PS‐NH3‐GalNH2), have been obtained. Differences in cell morphology, urea and plasma Fibronectin (pFN) production were clearly observed on HepG2 seeded on PS‐NH3 and PS‐NH3‐GalNH2. These results highlight the role of specific and non specific cell response in the in vitro study of materials intended to be used for biomedical purposes. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010.
Asymptomatic deep vein thrombosis in advanced cancer patients: The value of venous sonography
Authors: Beck‐Razi Nira , Kuzmin Alexander , Koren Dorith , Sarig Galit , Brenner Benjamin , Haim Nissim , Gaitini Diana 
Published: March 2010

AbstractPurpose.Although guidelines for venous thromboembolism prevention are available, the implementation of anticoagulant prophylaxis in patients with advanced cancer has yet to be more clearly defined. We aim to determine the incidence of lower extremity deep vein thrombosis (DVT) diagnosed by Doppler sonography (USD) in asymptomatic nonambulatory patients with advanced cancer.Method.In a prospective study, 44 nonambulatory cancer patients with grade 3–4 World Health Organization performance status, asymptomatic for lower extremity DVT, underwent bilateral venous USD studies of the lower extremities. Different risk factors and laboratory data were registered and correlated with the incidence of DVT.Result.Asymptomatic DVT was detected in 15 of 44 patients (34%, 95% CI, 0.21–0.49). Twenty‐three percent of all patients had isolated deep calf vein thrombi and 11% of all patients had thrombi in the proximal veins. The only significant risk factor was the number of metastatic sites. DVT was found in 4 of 23 (17.4%) patients with one metastatic site as opposed to 11 of 21 (52.3%) with two or more sites (p < 0.01).Conclusion.USD of the lower extremities detected asymptomatic DVT in 34% of advanced nonambulatory cancer patients and may serve as an additional decision‐making tool in the consideration of anticoagulant therapy for this specific population. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound, 2010
Selectivity of N‐aroyl‐N′‐arylthioureas towards 2‐(1,3‐dioxo‐1H‐inden‐2(3H)‐ylidene)malononitrile. New synthesis of (Z)‐N‐((E)‐4‐amino‐1‐aryl‐5‐cyano‐6‐oxo‐1H‐indeno[1,2‐d][1,3]‐ thiazepin‐2(6H)‐ylidene)‐4‐arylamides of antitumor and antioxidant activities
Authors: Aly Ashraf A. , Brown Alan B. , Ramadan Mohamed , Abdel‐Aziz Mohamed , Abuo‐Rahma Gamal El‐Din A. A. , Radwan Mohamed F. , Gamal‐Eldeen Amira M. 
Published: March 2010

Abstract
magnified image The reaction between N‐aroyl‐N′‐arylthioureas with 2‐(1,3‐dioxoindan‐2‐ylidene)malononitrile furnished indeno[1,2‐d][1,3]thiazepines in 70–85% yields. The mechanism of the products' formation is discussed. Some of the products showed effective antitumor and antioxidant activities. The results revealed that compound indenothiazepine derivative showed a high inhibition of the cell growth of Hep‐G2 cells is compared with the growth of untreated control cells, as concluded from their low IC50 value 21.73 μM. On the other hand, two indenothiazepine derivatives have an effective antioxidant activity with SC50 values of 62.5 mM and 87.4 mM, respectively. J. Heterocyclic Chem., (2010).
Construction of 3,4‐dihydro‐1,2‐diazete ring through 4π electron cyclization of 4‐hydroxy‐2‐oxo‐2H chromene‐3‐carbaldehyde [(1E)‐arylmethylene] hydrazone
Authors: Pansuriya Akshay M. , Savant Mahesh M. , Bhuva Chirag V. , Singh Jyoti , Kapuriya Naval , Naliapara Yogesh T. 
Published: March 2010

Abstract
magnified image A new, short and efficient synthesis of 4‐hydroxy‐3‐(4‐aryl‐3,4‐dihydro‐1,2‐diazet‐3‐yl)‐2H‐chromen‐2‐one is described in which the 3,4‐dihydro‐1,2‐diazete ring is constructed from arylmethylene hydrazone by 4π electron cyclization as per electrocyclic reaction. J. Heterocyclic Chem., (2010).
Synthesis, characterization and biological activity of99mTc‐labeled piperidine analogues targeting sigma receptors
Authors: Satpati Drishty , Bapat Ketaki , Sarma Haladhar Dev , Venkatesh Meera , Banerjee Sharmila 
Published: March 2010

AbstractSigma receptors are expressed in high density in various types of cancer cells including brain tumours and are also involved in various diseases of central nervous system. This makes ligands that bind to these receptors, attractive molecular vectors for targeting radiation to the specific sites with the purpose of imaging and therapy of neurological disorders. We report synthesis of three derivatives of 4‐amino‐N‐benzylpiperidine namely, 4‐dithiocarbamato‐N‐benzylpiperidine, 4‐iminodiacetato‐N‐benzylpiperidine and 4‐(N‐benzylpiperidine)‐pyridin‐2‐ylmethyl‐amino)‐acetic acid and their radiolabeling with technetium‐99m. Thein vivoevaluation of these radiolabeled compounds has been carried out in mice, for assessment of their binding affinity with sigma receptors. Of the three complexes, [99mTcN]‐4‐dithiocarbamato‐N‐benzylpiperidine, [99mTcN]Pip‐DTC exhibited the most promising characteristics with brain uptake of 0.6% ID/g at 5 min.p.i. that reduced to 0.3% ID/g after 2 h.p.i. Competition experiment carried out with [99mTcN]Pip‐DTC complex, using (+)‐pentazocine showed its specificity towards sigma receptors, as was found to be evident from reduction in the brain uptake of this complex. Introduction of iminodiacetate and pyridine moieties and subsequent radiolabeling did not result in complexes with significant potential of targeting and binding with sigma receptors. Copyright © 2010 John Wiley & Sons, Ltd.