bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular diagnosis and characterization of Anaplasma marginale and Ehrlichia ruminantium infecting beef cattle of Maputo Province, Mozambique
Authors: Matos Carlos António , Nomboro Cesária Fiossiane , Gonçalves Luiz Ricardo , Cala Aida Cristina , Sitoe Carlos Francisco , Vinte Ana Paula Rúpia , Mondlane Cristovão Mario , André Marcos Rogério , do Carmo Carrilho Maria 
Published: May 2024

Abstract
Background
Members of the Anaplasmataceae family, such as the Anaplasma and Ehrlichia species, cause economic losses and public health risks. However, the exact economic impact has not been comprehensively assessed in Mozambique due to limited data available on its basic epidemiology. Therefore, we investigated the molecular occurrence and identity of Anaplasma and Ehrlichia spp. infecting beef cattle in Maputo province, Mozambique.

Methods
A total of 200 whole blood samples were collected from apparently healthy beef cattle. Whole blood DNA was extracted and tested for presence of Anaplasma spp. and Ehrlichia ruminantium DNA through amplification of the 16S rRNA and map1 genes. Positive samples to Anaplasma spp. were subject to PCR assay targeting the A. marginale-msp5 gene. Amplicons obtained were purified, sequenced and subject to phylogenetic analyses.

Results
Anaplasma spp., A. marginale and E. ruminantium were detected in 153 (76.5%), 142 (71%) and 19 (9.5%) of all the samples analyzed, respectively. On this same sample group, 19 (9.5%) were co-infected with A. marginale and E. ruminantium. The 16S rRNA sequences of Anaplasma spp. obtained were phylogenetically related to A. marginale, A. centrale and A. platys. Phylogenetic analysis revealed that A. marginale-msp5 nucleotide sequences were grouped with sequences from Asia, Africa and Latin America, whereas E. ruminantium-map1 DNA nucleotide sequences were positioned in multiple clusters.

Conclusion
Cattle in Maputo Province are reservoirs for multiple Anaplasma species. A high positivity rate of infection by A. marginale was observed, as well as high genetic diversity of E. ruminantium. Furthermore, five new genotypes of E. ruminantium-map1 were identified.
PI3K/AKT pathway as a pivotal regulator of epithelial-mesenchymal transition in lung tumor cells
Authors: Moghbeli Meysam 
Published: May 2024

AbstractLung cancer, as the leading cause of cancer related deaths, is one of the main global health challenges. Despite various progresses in diagnostic and therapeutic methods, there is still a high rate of mortality among lung cancer patients, which can be related to the lack of clinical symptoms to differentiate lung cancer from the other chronic respiratory disorders in the early tumor stages. Most lung cancer patients are identified in advanced and metastatic tumor stages, which is associated with a poor prognosis. Therefore, it is necessary to investigate the molecular mechanisms involved in lung tumor progression and metastasis in order to introduce early diagnostic markers as well as therapeutic targets. Epithelial-mesenchymal transition (EMT) is considered as one of the main cellular mechanisms involved in lung tumor metastasis, during which tumor cells gain the metastatic ability by acquiring mesenchymal characteristics. Since, majority of the oncogenic signaling pathways exert their role in tumor cell invasion by inducing the EMT process, in the present review we discussed the role of PI3K/AKT signaling pathway in regulation of EMT process during lung tumor metastasis. It has been reported that the PI3K/AKT acts as an inducer of EMT process through the activation of EMT-specific transcription factors in lung tumor cells. MicroRNAs also exerted their inhibitory effects during EMT process by inhibition of PI3K/AKT pathway. This review can be an effective step towards introducing the PI3K/AKT pathway as a suitable therapeutic target to inhibit the EMT process and tumor metastasis in lung cancer patients.
Keratin 17 modulates the immune topography of pancreatic cancer
Authors: Delgado-Coka Lyanne , Horowitz Michael , Torrente-Goncalves Mariana , Roa-Peña Lucia , Leiton Cindy V. , Hasan Mahmudul , Babu Sruthi , Fassler Danielle , Oentoro Jaymie , Bai Ji-Dong K , Petricoin Emanuel F. , Matrisian Lynn M. , Blais Edik Matthew , Marchenko Natalia , Allard Felicia D. , Jiang Wei , Larson Brent , Hendifar Andrew , Chen Chao , Abousamra Shahira , Samaras Dimitris , Kurc Tahsin , Saltz Joel , Escobar-Hoyos Luisa F. , Shroyer Kenneth R. 
Published: May 2024

Abstract
Background
The immune microenvironment impacts tumor growth, invasion, metastasis, and patient survival and may provide opportunities for therapeutic intervention in pancreatic ductal adenocarcinoma (PDAC). Although never studied as a potential modulator of the immune response in most cancers, Keratin 17 (K17), a biomarker of the most aggressive (basal) molecular subtype of PDAC, is intimately involved in the histogenesis of the immune response in psoriasis, basal cell carcinoma, and cervical squamous cell carcinoma. Thus, we hypothesized that K17 expression could also impact the immune cell response in PDAC, and that uncovering this relationship could provide insight to guide the development of immunotherapeutic opportunities to extend patient survival.

Methods
Multiplex immunohistochemistry (mIHC) and automated image analysis based on novel computational imaging technology were used to decipher the abundance and spatial distribution of T cells, macrophages, and tumor cells, relative to K17 expression in 235 PDACs.

Results
K17 expression had profound effects on the exclusion of intratumoral CD8+ T cells and was also associated with decreased numbers of peritumoral CD8+ T cells, CD16+ macrophages, and CD163+ macrophages (p < 0.0001). The differences in the intratumor and peritumoral CD8+ T cell abundance were not impacted by neoadjuvant therapy, tumor stage, grade, lymph node status, histologic subtype, nor KRAS, p53, SMAD4, or CDKN2A mutations.

Conclusions
Thus, K17 expression correlates with major differences in the immune microenvironment that are independent of any tested clinicopathologic or tumor intrinsic variables, suggesting that targeting K17-mediated immune effects on the immune system could restore the innate immunologic response to PDAC and might provide novel opportunities to restore immunotherapeutic approaches for this most deadly form of cancer.
Association between the survey-based women’s empowerment index (SWPER) and intimate partner violence in sub-Saharan Africa
Authors: Donkoh Irene Esi , Aboagye Richard Gyan , Okyere Joshua , Seidu Abdul-Aziz , Ahinkorah Bright Opoku , Yaya Sanni 
Published: May 2024

AbstractBackgroundIntimate partner violence (IPV) is high among women of reproductive age in sub-Saharan Africa (SSA). However, empowering women enables them to confront and mitigate IPV. In this study, we examined the association between the survey-based women's empowerment index (SWPER) and IPV in SSA.MethodsWe used data from the Demographic and Health Surveys of 19 countries conducted from 2015 to 2021. Our study was restricted to a weighted sample of 82,203 women of reproductive age who were married or cohabiting. We used spatial maps to show the proportions of women who experienced past-year IPV. A five-modelled multilevel binary logistic regression analysis was adopted to examine the association between SWPER and IPV. The results were presented using the adjusted odds ratio (AOR) with their respective 95% confidence interval (CI). Statistical significance was set at p < 0.05.ResultsWith physical and emotional violence, the country with the highest prevalence was Sierra Leone, with a prevalence of 39.00% and 38.97% respectively. Rwanda (10.34%), Zambia (11.09%), Malawi (15.00%), Uganda (16.88%), and Burundi (20.32%) were the hotspot countries for sexual violence. Angola (34.54%), Uganda (41.55%), Liberia (47.94%), and Sierra Leone (59.98%) were the hotspot countries for IPV. A high SWPER score in attitudes to violence significantly decreased the odds of IPV [AOR = 0.70; 95% CI = 0.66, 0.75]. Also, women with medium score in decision-making were less likely to experience IPV compared to those with lower scores [AOR = 0.89; 95% CI = 0.83, 0.95]. However, higher odds of experiencing IPV was found among women with medium score in autonomy compared to those with low scores [AOR = 1.07; 95% CI = 1.01, 1.14].ConclusionsOur study has shown that the three dimensions of SWPER significantly predict IPV among women. Consequently, it is crucial that sub-Saharan African countries implement various initiatives, such as IPV advocacy programs and economic livelihood empowerment initiatives. These initiatives should not only aim to improve women's attitudes to domestic violence but also to enhance their social independence, autonomy, and decision-making capacity.
LncRNA NUTM2A-AS1 silencing inhibits glioma via miR-376a-3p/YAP1 axis
Authors: Zeng Yuecheng , Yang Zhenyu , Yang Yang , Wang Peng 
Published: May 2024

AbstractThe lncRNA NUTM2A-AS1 has been shown to be dysregulated in gastric cancer, while the roles in glioma is unclear. The aim of this study was to investigate the roles and potential mechanisms of lncRNA NUTM2A-AS1 in the proliferation and apoptosis of glioma cells. The StarBase software and dual luciferase reporter assay were used to identify the relationship between lncRNA NUTM2A-AS1 and miR-376a-3p, and miR-376a-3p and YAP1. The expression of lncRNA NUTM2A-AS1, miR-376a-3p, and YAP1 in human glioma cell lines was detected by qRT-PCR. MTT and flow cytometry were used to detect the effects of lncRNA NUTM2A-AS1 or miR-376a-3p on the proliferation and apoptosis of U251 and A172 cells, respectively. In addition, changes of Bax and Bcl-2 expression in glioma cells were further verified by western blotting and qRT-PCR. The results showed that the expression of lncRNA NUTM2A-AS1 was elevated in glioma cell lines, while miR-376a-3p was decreased. LncRNA NUTM2A-AS1 was negatively correlated with miR-376a-3p. Silencing of lncRNA NUTM2A-AS1 enhanced the levels of miR-376a-3p, leading to reduced cell proliferation and increased apoptosis in glioma cells. YAP1 was a direct target of miR-376a-3p, and it was negatively regulated by miR-376a-3p in U251 and A172 cells. Further mechanistic studies suggested that miR-376a-3p reduced glioma cell proliferation and increased apoptosis by inhibiting YAP1 expression. In addition, lncRNA NUTM2A-AS1 positively regulated of YAP1 expression in glioma cells. In conclusion, silencing of lncRNA NUTM2A-AS1 inhibited proliferation and induced apoptosis in human glioma cells via the miR-376a-3p/YAP1 axis.
Total knee arthroplasty: does ultra-early physical therapy improve functional outcomes and reduce length of stay? A retrospective cohort study
Authors: Thwin Lynn , Chee Brian Rui Kye , Yap Yan Mei , Tan Kelvin Guoping 
Published: May 2024

Abstract
Background
The Enhanced Recovery After Surgery (ERAS) Society recommends that after total knee arthroplasty (TKA), patients should be mobilized early. However, there is no consensus on how early physical therapy should be commenced. We aim to investigate whether ultra-early physical therapy (< 12 h postoperatively) leads to better outcomes.

Methods
This is a retrospective cohort study of 569 patients who underwent primary TKA from August 2017 to December 2019 at our institution. We compared patients who had undergone physical therapy either within 24 h or 24–48 h after TKA. Further subgroup analysis was performed on the < 24 h group, comparing those who had undergone PT within 12 h and within 12–24 h. The outcomes analyzed include the Oxford Knee Scoring System score, Knee Society Scores, range of motion (ROM), length of stay (LOS) and ambulatory distance on discharge. A student’s t test, chi-squared test or Fisher’s exact test was used where appropriate, to determine statistical significance of our findings.

Results
LOS in the < 24 h group was shorter compared to the 24–48 h group (4.87 vs. 5.34 days, p = 0.002). Subgroup analysis showed that LOS was shorter in the ultra-early PT (< 12 h) group compared to the early PT (12–24 h) group (4.75 vs. 4.96 days, p = 0.009). At 3 months postoperatively, there was no significant difference in ROM, ambulatory distance or functional scores between the < 24 h group and 24–48 h group, or on subgroup analysis of the < 24 h group.

Conclusion
Patients who underwent physical therapy within 24 h had a shorter length of stay compared to the 24–48 h group. On subgroup analysis, ultra-early (< 12 h) physical therapy correlated with a shorter length of stay compared to the 12–24 h group (4.75 vs. 4.96 days, p = 0.009) - however, the difference is small and unlikely to be clinically significant. Ultra-early (< 12 h) physical therapy does not confer additional benefit in terms of functional scores, ROM or ambulatory distance. These findings reinforce the importance of early physical therapy after TKA in facilitating earlier patient discharge.
The prevalence of clinically relevant delayed intracranial hemorrhage in head trauma patients treated with oral anticoagulants is very low: a retrospective cohort register study
Authors: André Lars , Björkelund Anders , Ekelund Ulf , Vedin Tomas , Björk Jonas , Forberg Jakob Lundager 
Published: May 2024

Abstract
Background
Current guidelines from Scandinavian Neuro Committee mandate a 24-hour observation for head trauma patients on anticoagulants, even with normal initial head CT scans, as a means not to miss delayed intracranial hemorrhages. This study aimed to assess the prevalence, and time to diagnosis, of clinically relevant delayed intracranial hemorrhage in head trauma patients treated with oral anticoagulants.

Method
Utilizing comprehensive two-year data from Region Skåne’s emergency departments, which serve a population of 1.3 million inhabitants, this study focused on adult head trauma patients prescribed oral anticoagulants. We identified those with intracranial hemorrhage within 30 days, defining delayed intracranial hemorrhage as a bleeding not apparent on their initial CT head scan. These cases were further defined as clinically relevant if associated with mortality, any intensive care unit admission, or neurosurgery.

Results
Out of the included 2,362 head injury cases (median age 84, 56% on a direct acting oral anticoagulant), five developed delayed intracranial hemorrhages. None of these five cases underwent neurosurgery nor were admitted to an intensive care unit. Only two cases (0.08%, 95% confidence interval [0.01–0.3%]) were classified as clinically relevant, involving subdural hematomas in patients aged 82 and 87 years, who both subsequently died. The diagnosis of these delayed intracranial hemorrhages was made at 4 and 7 days following initial presentation to the emergency department.

Conclusion
In patients with head trauma, on oral anticoagulation, the incidence of clinically relevant delayed intracranial hemorrhage was found to be less than one in a thousand, with detection occurring four days or later after initial presentation. This challenges the effectiveness of the 24-hour observation period recommended by the Scandinavian Neurotrauma Committee guidelines, suggesting a need to reassess these guidelines to optimise care and resource allocation.

Trial registration
This is a retrospective cohort study, does not include any intervention, and has therefore not been registered.
Updated safety results from phase 3 lecanemab study in early Alzheimer’s disease
Authors: Honig Lawrence S. , Sabbagh Marwan N. , van Dyck Christopher H. , Sperling Reisa A. , Hersch Steven , Matta Andre , Giorgi Luigi , Gee Michelle , Kanekiyo Michio , Li David , Purcell Derk , Dhadda Shobha , Irizarry Michael , Kramer Lynn 
Published: May 2024

Abstract
Background
Alzheimer disease (AD) is a major health problem of aging, with tremendous burden on healthcare systems, patients, and families globally. Lecanemab, an FDA-approved amyloid beta (Aβ)-directed antibody indicated for the treatment of early AD, binds with high affinity to soluble Aβ protofibrils, which have been shown to be more toxic to neurons than monomers or insoluble fibrils. Lecanemab has been shown to be well tolerated in multiple clinical trials, although risks include an increased rate of amyloid-related imaging abnormalities (ARIA) and infusion reactions relative to placebo.

Methods
Clarity AD was an 18-month treatment (Core study), multicenter, double-blind, placebo-controlled, parallel-group study with open-label extension (OLE) in participants with early AD. Eligible participants were randomized 1:1 across 2 treatment groups (placebo and lecanemab 10 mg/kg biweekly). Safety evaluations included monitoring of vital signs, physical examinations, adverse events, clinical laboratory parameters, and 12-lead electrocardiograms. ARIA occurrence was monitored throughout the study by magnetic resonance imaging, read both locally and centrally.

Results
Overall, 1795 participants from Core and 1612 participants with at least one dose of lecanemab (Core + OLE) were included. Lecanemab was generally well-tolerated in Clarity AD, with no deaths related to lecanemab in the Core study. There were 9 deaths during the OLE, with 4 deemed possibly related to study treatment. Of the 24 deaths in Core + OLE, 3 were due to intracerebral hemorrhage (ICH): 1 placebo in the Core due to ICH, and 2 lecanemab in OLE with concurrent ICH (1 on tissue plasminogen activator and 1 on anticoagulant therapy). In the Core + OLE, the most common adverse events in the lecanemab group (> 10%) were infusion-related reactions (24.5%), ARIA with hemosiderin deposits (ARIA-H) microhemorrhages (16.0%), COVID-19 (14.7%), ARIA with edema (ARIA-E; 13.6%), and headache (10.3%). ARIA-E and ARIA-H were largely radiographically mild-to-moderate. ARIA-E generally occurred within 3–6 months of treatment, was more common in ApoE e4 carriers (16.8%) and most common in ApoE ε4 homozygous participants (34.5%).

Conclusions
Lecanemab was generally well-tolerated, with the most common adverse events being infusion-related reactions, ARIA-H, ARIA-E. Clinicians, participants, and caregivers should understand the incidence, monitoring, and management of these events for optimal patient care.

Trial registration
ClinicalTrials.gov numbers: Clarity AD NCT03887455)
On a class of a coupled nonlinear viscoelastic Kirchhoff equations variable-exponents: global existence, blow up, growth and decay of solutions
Authors: Choucha Abdelbaki , Haiour Mohamed , Boulaaras Salah 
Published: May 2024

AbstractIn this work, we consider a quasilinear system of viscoelastic equations with dispersion, source, and variable exponents. Under suitable assumptions on the initial data and the relaxation functions, we obtained that the solution of the system is global and bounded. Next, the blow-up is proved with negative initial energy. After that, the exponential growth of solutions is showed with positive initial energy, and by using an integral inequality due to Komornik, the general decay result is obtained in the case of absence of the source term.
Nonsmooth dynamics of a Filippov predator–prey ecological model with antipredator behavior
Authors: Huang Lidong , Qin Wenjie , Chen Shuai 
Published: May 2024

AbstractThis article proposes a class of nonsmooth Filippov pest–predator ecosystems with intermittent control strategies based on the pest’s antipredator behavior. aiming to investigate the influence of control strategies and switching thresholds on pest control. First, a comprehensive theoretical analysis of various equilibria within the Filippov system is undertaken, emphasizing the presence and stability of sliding mode dynamics and pseudoequilibrium. Secondly, through numerical simulations, the article discusses boundary-focus, boundary-node, and boundary-saddle bifurcation. Finally, the nonexistence of limit cycles in the Filippov system is theoretically studied. The research indicates that the solution trajectories of the model ultimately stabilize either at the real equilibria or at pseudoequilibrium on the model’s switching surface. Moreover, when the model has multiple coexisting real equilibrium and pseudoequilibrium, the pest-control strategy is correlated with the initial density of both the pest and the predator population.
Perceived ageism and psychosocial outcomes during the COVID-19 pandemic
Authors: Hajek André , König Hans-Helmut 
Published: May 2024

Abstract
Background
In light of the existing knowledge gap in this research area (particularly based on representative samples and research conducted during the pandemic), the objective of this study was to explore the association between perceived ageism and psychosocial outcomes (i.e., in terms of life satisfaction, loneliness, social isolation, aging satisfaction and depressive symptoms) among middle-aged and older adults during the COVID-19 pandemic based on nationally representative data.

Methods
We used data from the nationally representative German Ageing Survey, which covers community-dwelling middle-aged and older individuals aged 40 years or over. Specifically, wave 7 of the German Ageing Survey (conducted from November 2020 to March 2021) was analyzed, consisting of a sample of 4,167 individuals with an average age of 68.7 years (SD: 10.1 years; ranging from 46 to 98 years). Established instruments were employed to measure psychosocial outcomes.

Results
Regressions showed that the presence of perceived ageism was significantly associated with unfavorable psychometric outcomes (i.e., higher loneliness: β = 0.29, p < 0.001; higher perceived social isolation: β = 0.32, p < 0.001; more depressive symptoms: β = 2.68, p < 0.001; lower life satisfaction: β=-0.28, p < 0.001; higher negative affect: β = 0.21, p < 0.001; lower aging satisfaction: β=-0.19, p < 0.001), except for positive affect (β=-0.06, p = 0.10). Stratified by age group (i.e., individuals 40 to 64 years; individuals aged 65 years and over, see Tables 3 and 4), comparable results were obtained in terms of significance.

Conclusions
Perceived ageism was associated with unfavorable psychosocial outcomes. This knowledge can help reduce vulnerability to negative psychosocial factors in people in the middle and later years of life.
Curie point depth, thermal gradient and heat flow along the Ethiopia Rift System and adjacent plateaus using spectral evaluation approach: implications for geothermal resources
Authors: Hilemichaeil Samson , Haile Tigistu , Yirgu Gezahegn 
Published: May 2024

AbstractThe Ethiopia Rift System (ERS) is a section of the East African Rift System within Ethiopia extending from the Afar in the northeast to the Kenya border in the southwest. It is apparent that magmatism and magmatic intrusions influence the crustal shape in the ERS resulting in its thinning and the shallowing of magmatic sources at various locations within it. As a consequence, more than 31 volcanoes hosting hydrothermal structures with a conceivable potential to generate massive quantities of geothermal energy have been identified along the ERS. In this study, we map the Curie Point Depth (CDP) over the ERS based on the analysis of aeromagnetic data extracted from the World Digital Magnetic Anomaly Map. Spectral evaluation method was used to estimate the boundaries (top and bottom) of the magnetized crust. Reduced-to-pole (RTP) aeromagnetic records have been divided into 105 (50% overlap) square blocks of 200 × 200 km size. The Curie temperature (580 °C) of magnetite was used to determine the thermal gradient and the heat drift in the area. The depths obtained for the bottom of the magnetized crust are assumed to correspond to the Curie Depths, where the magnetic layer loses all its magnetization. The determined values of Curie Point Depth, geothermal gradient and heat flow for the 50% overlapped 105 blocks, respectively, range from 8.85 to 55.85 km, 10.38 to 65.54 °C/km and 25.96 to 163.84 mW/m2. Lower CPD (< 20 km) in the ERS was obtained between Mille and Gewane (southwest Afar), between Adama (Nazret) and Yerer (NMER) and between Wendo Genet and Koti (SMER) localities. These areas, showing low CPD, exhibit excessive geothermal gradient and high heat flow all of which indicate the presence of significant geothermal potential.