bluuebunny/crossref_metadata_2025_split huggingface dataset:
Two laccase isoforms of the basidiomycete Cerrena unicolor VKMF‐3196. Induction, isolation and properties
Authors: Lisova Zoya Alexandrovna , Lisov Alexander Viktorovich , Leontievsky Alexey Arkadievich 
Published: February 2010

AbstractThe laccase induction in submerged culture of basidiomycete Cerrena unicolor VKM F‐3196 was investigated. Cu2+ at concentration 0.1 mM was an optimum inducer of C. unicolor laccase. Two isoforms of laccase, namely LacC1 and LacC2, were isolated and characterized. The isoforms were shown to have different physical‐chemical and catalytic properties. On the basis of the MALDI TOF MS analysis of tryptic cleavage products of both the proteins and N‐terminal amino‐acid sequences analysis two isoforms of laccase (LacC1 and LacC2) were classified as products of two different genes. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Raman microscopy of prehistoric rock paintings from the Hoz de Vicente, Minglanilla, Cuenca, Spain
Authors: Hernanz Antonio , Ruiz‐López Juan F. , Gavira‐Vallejo José M. , Martin Santiago , Gavrilenko Egor 
Published: February 2010

AbstractThe third painted panel of the Hoz de Vicente rock shelter (Minglanilla, Cuenca, Spain) contains a series of prehistoric pictographs which were studied by Raman microscopy. Scanning electron microscopy (SEM), energy dispersive X‐ray microanalysis (EDX) and petrographic polarised light microscopy were used as auxiliary techniques. The results obtained indicate that well‐crystallised haematite (α‐Fe2O3) of grain size less than 1 µm was used as red pigment. However, amorphous carbon, probably vegetable charcoal or soot, was used as the black pigment. A patina of whewellite and weddellite covers the areas of the painting panel with pictographs. The microstratigraphic study of a schematic figure revealed that the layer of pigment is sandwiched between layers of these hydrated forms of calcium oxalate produced by the activity of fungi and lichens. These products have also been detected in the layer of pigment. These findings will enable obtaining limiting radiocarbon14C accelerator mass spectrometry (AMS) dates for the corresponding pictorial events. On the other hand, crystallisation of gypsum in the external layers of the panel is related to the deterioration observed in the flaking areas. Gypsum and clayish minerals appear as the main components of an ochre‐coloured accretion covering several parts of the panel. Copyright © 2010 John Wiley & Sons, Ltd.
Incident circularly polarized Raman optical activity spectrometer based on circularity conversion method
Authors: Yamamoto Shigeki , Watarai Hitoshi 
Published: February 2010

AbstractA simple incident circular polarization Raman optical activity (ICP ROA) spectrometer was constructed by applying the method of circularity conversion. The circular polarization of the incident laser light was modulated between right and left by the insertion of a half‐wave plate and not by using a Pockels cell which is usually used in ICP ROA instruments. On the basis of the concept of the virtual enantiomer (Hug, W. Applied Spectroscopy, 2003, 57, 1), circularity converters were inserted in the optical train, which could effectively compensate the systematic offset. The new instrument successfully attained photon shot‐noise‐limited conditions for all bands except for the very strongly polarized Raman band. The ROA spectra of some standard chiral samples were measured to demonstrate the performance of the spectrometer. Copyright © 2010 John Wiley & Sons, Ltd.
Frontotemporal dementia: Pathology of gait?
Authors: Allali Gilles , Dubois Bruno , Assal Frederic , Lallart Elise , de Souza Leonardo C. , Bertoux Maxime , Annweiler Cédric , Herrmann Francois R. , Levy Richard , Beauchet Olivier 
Published: February 2010

AbstractThe main diagnostic criteria of the behavioural variant of frontotemporal degeneration (bvFTD) include neurobehavioral and dysexecutive syndromes, but not specific gait characteristics although strong relationship between gait and prefrontal functions are increasingly recognized. Accordingly, we tested the hypothesis that patients with bvFTD would have more gait changes than older healthy controls and demented patients with Alzheimer's disease (AD). Sixty subjects were included in the study: 19 with bvFTD, 19 with AD and 22 healthy controls. Mean values and coefficients of variation (CV) of stride time while just walking (i.e., single tasking) and while walking with backward counting (i.e., dual tasking) were measured using the SMTEC®‐footswitch system. Stride time, mean value, and CV were significantly increased in both patient groups compared with healthy controls during single task or walking alone (P < 0.001) and during dual tasking (P < 0.001). After adjusting for age, Mini‐mental examination, psychoactive drugs, gender, and history of previous fall, only the patients with bvFTD group was associated with an increase of CV of stride time during single walking (P < 0.001) and dual tasking (P < 0.001). These data suggest that gait instability during single and dual tasking could represent a supportive argument for bvFTD. In clinical practice, such a diagnosis should be at least considered in any demented patient with gait instability. © 2010 Movement Disorder Society
In Pursuit of Fully Flexible Protein‐Ligand Docking: Modeling the Bilateral Mechanism of Binding
Authors: Henzler Angela M. , Rarey Matthias 
Published: February 2010

AbstractModern structure‐based drug design aims at accounting for the intrinsic flexibility of therapeutic relevant targets. Over the last few years a considerable amount of docking approaches that encounter this challenging problem has emerged. Here we provide the readership with an overview of established methods for fully flexible protein‐ligand docking and current developments in the field. All methods are based on one of two fundamental models which describe the dynamic behavior of proteins upon ligand binding. Methods for ensemble docking (ED) model the protein conformational change before the ligand is placed, whereas induced‐fit docking (IFD) optimizes the protein structure afterwards. A third category of docking approaches is formed by recent approaches that follow both concepts. This categorization allows to comprehensively discover strengths and weaknesses of the individual processes and to extract information for their applicability in real world docking scenarios.
Assessment of therapeutic response of locally advanced breast cancer (LABC) patients undergoing neoadjuvant chemotherapy (NACT) monitored using sequential magnetic resonance spectroscopic imaging (MRSI)
Authors: Danishad Karikanni Kalathil A. , Sharma Uma , Sah Rani G. , Seenu Vurthaluru , Parshad Rajinder , Jagannathan Naranamangalam R. 
Published: February 2010

AbstractThe potential of total choline (tCho) signal‐to‐noise ratio (SNR) (ChoSNR) and tumor volume in the assessment of tumor response in locally advanced breast cancer (LABC) patients (n = 30) undergoing neoadjuvant chemotherapy (NACT) was investigated using magnetic resonance spectroscopic imaging (MRSI) and conventional MRI at 1.5 T. Experiments were carried out sequentially at four time‐points: prior to therapy and after I, II and III NACT and ChoSNR, and the tumor volume was measured. The MR response was compared with the clinical response. Sequential data of 25 patients were retrospectively analyzed by classifying them as clinical responders and non‐responders. In 14 responders, the pre‐therapy ChoSNR was 7.8 ± 5.1. In 10/14 responders, no choline was observed after III NACT while in the remaining four patients the ChoSNR was reduced to 3.6 ± 1.1 (p < 0.05). Non‐responders showed no statistically significant change in ChoSNR. After III NACT, the tumor volume reduced by 84.0 ± 14.8% in responders. Using receiver operating curve (ROC) analysis, cut‐off values of 53% for ChoSNR and 47.5% for volume were obtained to differentiate responders from non‐responders. The sensitivity to detect responders from non‐responders using ChoSNR was 85.7% with 91% specificity while 100% sensitivity was observed for volume but with reduced specificity of 73%. Our results indicate that ChoSNR may serve as a useful parameter to predict tumor response to NACT with higher specificity compared to volume, suggesting its potential in effective treatment management. Copyright © 2010 John Wiley & Sons, Ltd.
DTI at long diffusion time improves fiber tracking
Authors: Rane Swati , Nair Govind , Duong Timothy Q. 
Published: February 2010

AbstractWhile diffusion‐tensor‐imaging tractography provides remarkable in vivo anatomical connectivity of the central nervous system, the majority of DTI studies to date are predominantly limited to tracking large white‐matter fibers. This study investigated DTI tractography using long diffusion time (tdiff) to improve tracking of thinner fibers in fixed rhesus monkey brains. Stimulated Echo Acquisition Mode (STEAM) sequence on a 3T Siemens TRIO was modified to include a diffusion module. DTI was acquired using STEAM with tdiff of 48 and 192 ms with matched signal‐to‐noise ratios (SNR). Comparisons were also made with the conventional double‐spin echo (DSE) at a short tdiff of 45 ms. Not only did the fractional anisotropy increase significantly with the use of long diffusion time, but directional entropy measures indicated that there was an increased coherence amongst neighboring tensors. Further, the magnitude of the major eigenvector was larger at the tdiff = 192 ms as compared to the short tdiff. Probabilistic connectivity maps at long tdiff showed larger areas of connectivity with the use of long diffusion time, which traversed deeper into areas of low anisotropy. With tractography, it was found that the length of the fibers, increased by almost 10% in the callosal fibers that branch into the paracentral gyrus, the precentral gyrus and the post central gyrus. A similar increase of about 20% was observed in the fibers of the internal capsule. These findings offer encouraging data that DTI at long diffusion time could improve tract tracing of small fibers in areas of low fractional anisotropy (FA), such as at the interfaces of white matter and grey matter. Copyright © 2010 John Wiley & Sons, Ltd.
Data management and functional annotation of the Korean reference plasma proteome
Authors: Jeong Seul‐Ki , Lee Eun‐Young , Cho Jin‐Young , Lee Hyoung‐Joo , Jeong An‐Sung , Cho Sang Yun , Paik Young‐Ki 
Published: February 2010

AbstractAs human plasma is clinically valuable, reference data from healthy donors can be a useful source for serological biomarker studies. To make a reliable protein catalog of the Korean plasma proteome, various experimental methods, such as 1‐D HPLC, 2‐D LC, and narrow ranged 2‐DE prior to MALDI‐TOF and LC‐MS/MS, were used to identify unique plasma proteins in this population. To compile candidates with high confidence, two different search engines were used to select proteins with a false discovery rate of less than or equal to 1%. From this rigorous selection process, we initially identified 494 distinct Korean plasma proteins. After multilevel stepwise filtrations with stringent, identification parameters were applied to acquire plasma protein list with the maximum confidence; a total 185 distinct plasma proteins were identified and integrated into our Korean human plasma proteome project database along with several bioinformatics analysis results, including gene ontology, biological pathways, tissue expression, and disease association. This is the first publicly available single ethnic group‐specific plasma proteome database (http://proteomix.org/khppp/).
Computational study of the cooperative effects of nitrogen and silicon atoms on the singlet–triplet energy spacing in 1,3‐diradicals and the reactivity of their singlet states
Authors: Nakamura Takeshi , Gagliardi Laura , Abe Manabu 
Published: February 2010

AbstractQuantum chemical calculations were performed to investigate the cooperative effect of the nitrogen and silicon atoms on the singlet–triplet energy spacing and the reactivity of the singlet state in 1,2‐diazacyclopentane‐3,5‐diyls and 1,2‐diaza‐4‐silacyclopentane‐3,5‐diyls. The largest singlet–triplet energy gap (${\rm }\Delta E_{{\rm ST}}^{\rm c}$ = −36.1 kcal/mol) found so far in localized 1,3‐diradicals was in the C2v symmetry of 4,4‐difluoro‐1,2‐diaza‐4‐silacyclopentane‐3,5‐diyl at the UB3LYP/6‐31G(d) level of theory. The cooperative effect was also found in the energy differences of singlet diradicals with the corresponding ring‐closing compounds, bicyclo[2.1.0]pentane derivatives. The singlet state of the 1,2‐diaza‐4‐silacyclopentane‐3,5‐diyls was calculated to be energetically more stable than the ring‐closing compound. The notable finding on the stability of the singlet diradicals may be attributed to the resonance structures that specifically stabilize the singlet state of diradicals. The computational studies predict that the singlet 1,2‐diaza‐4‐silacyclopentane‐3,5‐diyl is a persistent molecule under conditions without intermolecular‐trapping reagents. Copyright © 2010 John Wiley & Sons, Ltd.
Dehydropericyclic routes to reactive intermediates
Authors: Johnson Richard P. 
Published: February 2010

AbstractDehydropericyclic reactions are conceptually derived from pericyclic reactions by systematic removal of hydrogen atom pairs. Whereas pericyclic reactions usually involve kinetically stable reactants and products, dehydropericyclic reactions often result from or generate strained reactive intermediates. Nevertheless, such reactions are scattered widely through the chemical literature. The dehydropericyclic concept provides a straightforward approach for inventing new chemical reactions, especially routes to reactive intermediates, and for fitting other known processes into a broader framework. Copyright © 2010 John Wiley & Sons, Ltd.
Addition of SiF4 to standard SiH4+H2 plasma: an effective way to reduce oxygen contamination in μc‐Si:H films
Authors: Abramov Alexey , Cabarrocas Pere Roca i 
Published: February 2010

AbstractThe effect of adding of silicon tetrafluoride (SiF4) to silane (SiH4) hydrogen gas mixture on the properties of highly crystallized microcrystalline silicon (μc‐Si:H) films was studied. We found that the addition of a small amount of SiF4, with a ratio to SiH4 not exceeding 1/10, has almost no effect on deposition rate and does not affect the structure of the μc‐Si:H films, but strongly reduces oxygen incorporation into the films. Reduction of oxygen content to values below 1019 cm‐3 results in intrinsic character of the films with ratio of photo to dark conductivity about 103 in whole studied range of deposition temperatures from 175 to 250 °C in contrast to films, deposited without SiF4 addition, which show strong decrease of photosensitivity with increase of deposition temperature above 200 °C (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Structure and optical properties in the amorphous to crystalline transition in AgSbSe2 thin films
Authors: Hamam M. , El‐Gendy Y. A. , Selim M. S. , Teleb N. H. , Salem A. M. 
Published: February 2010

AbstractNearly stoichiometric thin films of the ternary AgSbSe2 compound have been deposited at room temperature by conventional thermal evaporation of the presynthesized material onto glass substrate. The X‐ray and electron diffraction studies revealed that the as‐deposited films are amorphous in nature, while an amorphous‐to‐crystalline phase transition could be obtained by thermal annealing at 373 K. The elemental chemical composition of as‐deposited films was confirmed using the energy dispersive X‐ray analysis. The transmission and reflection spectra of as‐deposited and annealed films at annealing different temperatures were recorded at normal light incidence in the wavelength range 600‐2500 nm. The refractive index and optical band gap have been calculated for the investigated films. The dispersion parameters, (Eo, Ed) static refractive index ns (0), static dielectric constant, ϵs and the carrier concentration to the effective mass ratio, N/m* have been calculated. An analysis of the optical absorption spectra revealed a non direct optical transition characterizing the as‐deposited films and those annealed at 343 and 374 K while; direct and indirect optical transitions characterized the films annealed at 398 K. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Characterization of microcrystalline Si films deposited at low temperatures with high rates by atmospheric‐pressure plasma CVD
Authors: Ouchi K. , Tabuchi K. , Ohmi H. , Kakiuchi H. , Yasutake K. 
Published: February 2010

AbstractLow‐temperature and high‐rate depositions of hydrogenated microcrystalline silicon (μc ‐Si:H) films are investigated using stable plasma excited at atmospheric pressure (AP) by supplying 150‐MHz very high‐frequency (VHF) power. VHF power density, H2/SiH4 ratio and plasma gap are varied as parameters under a fixed substrate temperature (Tsub) of 220 ΩC. Increasing VHF power density and H2/SiH4 ratio is primarily important for the sufficient decomposition of source gas molecules, improving both deposition rate and film property. In addition, the plasma gap is found to be another crucial parameter in the deposition process using AP‐VHF plasma. Numerical analyses on the temperature distribution around the plasma region have revealed that the steady‐state surface temperature of the glass substrate becomes approximately 100 ΩC higher than the back surface temperature (Tsub), which is caused by the moderate surface heating effect of the AP‐VHF plasma. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Plasma emission diagnostics during fast deposition of microcrystalline silicon thin films in matrix distributed electron cyclotron resonance plasma CVD system
Authors: Ram Sanjay K. , Kroely Laurent , Kasouit Samir , Bulkin Pavel , Cabarrocas Pere Roca i 
Published: February 2010

AbstractThe structural and optoelectronic properties of undoped hydrogenated microcrystalline silicon (μ c‐Si:H) films deposited in a matrix distributed electron cyclotron resonance (MDECR) plasma CVD system using pure silane were studied as a function of microwave power (PMW) and gas pressure (Pgas). The analysis of the silane plasma optical emission spectra (OES) shows the effect of the deposition conditions on the plasma chemistry and resulting film microstructure as studied by spectroscopic ellipsometry and atomic force microscopy. The use of the PMW and Pgas as tunable parameters to achieve μ c‐Si:H films with desired microstructure is discussed. Intrinsic μ c‐Si:H films with good phototransport properties were realized at high deposition rate. Our study shows the usefulness of OES as a fast diagnostic tool for controlling and optimizing the μ c‐Si:H deposition process in MDECR reactor. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Functional and Clinical Results of Transanal Endoscopic Microsurgery Combined with Endoscopic Posterior Mesorectum Resection for the Treatment of Patients with T1 Rectal Cancer
Authors: Walega Piotr , Kenig Jakub , Richter Piotr , Nowak Wojciech 
Published: February 2010

AbstractBackgroundRectum‐sparing transanal endoscopic microsurgery (TEM) is a well‐established treatment for T1 carcinomas of the rectum. However, it is associated with an increased rate of local recurrence compared with extended resection. In most cases, this failure is linked to the presence of clinically nondetectable metastases in the regional lymph nodes. Endoscopic posterior mesorectal resection (EPMR) makes it possible to remove the relevant lymphatic drainage of the lower third of the rectum in the minimally invasive way, which can help with adequate tumor staging. The study evaluated the influence of combined TEM and EPMR treatment on the anorectal functions of this group of patients.MethodsSix consecutive patients (3 women and 3 men; mean age, 71.3 years) with T1 cancer of the rectum were operated on using TEM in combination with EPMR as a two‐stage procedure between 2007 and 2009.ResultsAfter a median follow‐up of 19 (range, 8–30) months, none of our patients complained of symptoms of incontinence during the postoperative period apart from one woman with gas incontinence, who was diagnosed preoperatively. There was no statistically significant difference in BAP, SAP, HPZL, or in fecal continence control assessed using the Fecal Incontinence Severity Index before and 1, 3, and 6 months after the procedure. We observed one case of intraoperative complication (perforation) and one case of minor postoperative complication (hematoma formation). There was no evidence of locoregional recurrence.ConclusionsEPMR in combination with TEM seems to be safe, feasible, and with no impact on the basic anorectal functions.
Diagnostic efficacy of surgeon‐performed ultrasound‐guided fine needle aspiration: A randomized controlled trial
Authors: Robitschek Jon , Straub Mary , Wirtz Eric , Klem Christopher , Sniezek Joseph 
Published: February 2010

ObjectiveTo evaluate the clinical efficacy of surgeon‐performed, office‐based head and neck ultrasound in facilitating diagnostic fine needle aspiration (FNA) of lesions in the head and neck.Study DesignA randomized controlled trial of ultrasound‐guided FNA versus traditional palpation‐guided technique for palpable masses in the head and neck.SettingAn office‐based study performed in a military academic medical center.Subjects and MethodsEighty‐one adults older than 18 years of age with a palpable head and neck mass (less than 3 cm in largest diameter) were randomized to ultrasound‐guided or traditional palpation‐guided FNA of a head and neck mass. Measured variables and outcomes for the study included tissue adequacy rates, tissue type, and operator variability.ResultsFollowing three passes using either palpation or ultrasound guidance, a comparative tissue adequacy rate of 84 percent for ultrasound guidance versus 58 percent for standard palpation was established (P < 0.014). With regard to tissue type, a statistically significant comparative diagnostic advantage for ultrasound guidance was observed in thyroid tissue while remaining statistically insignificant for lymphatic and salivary tissues. No statistical significance was found when comparing the ability of otolaryngology residents versus attending otolaryngologists to obtain ultrasound‐guided diagnostic samples.ConclusionOffice‐based surgeon‐performed ultrasound‐guided FNA of palpable lesions in the head and neck yields a statistically significant higher diagnostic rate compared to standard palpation technique. Our institutional experience supports the utility of surgeon‐performed ultrasound as a core competency in clinical practice.
Outcomes of laser thermal therapy for recurrent head and neck cancer
Authors: Sercarz Joel A. , Bublik Michael , Joo Jayne , Paiva Paulo B. , Areco Kelsy N. , Brandalise Maria Helena , Loh Christopher , Masterman‐Smith Michael , Paiva Marcos B. 
Published: February 2010

ObjectiveTo review the outcomes of a phase II study using laser‐induced thermal therapy (LITT) as a palliative treatment for 106 patients with recurrent head and neck tumors.Study DesignRetrospective study.SettingTertiary hospital in the United States.Subjects and MethodsThe primary endpoints were tumor response and survival. Prognostic values were assessed by the Kaplan‐Meier method.ResultsThe best results were seen in oral cavity tumors, in which mean survival was 29.1 months, as compared to neck tumors (mean 14.4 ± 6.9 months; range 7.5‐20.7 months; with a 95% confidence interval). Further analysis showed that clinical factors such as gender, smoking, and alcohol use were not indicators of poor prognosis, whereas neck disease and tumor stage at first treatment were relevant factors.ConclusionIn this study, 40 out of 106 patients treated by LITT remained alive at the end of our follow‐up, and a complete response was seen in 24 (22.6%) patients. The highest response rate was seen in oral cavity tumors, which suggests that tumor location at this site may be a predictor of favorable outcome with LITT.