bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relationship between Serotypes, Age, and Clinical Presentation of Invasive Pneumococcal Disease in Madrid, Spain, after Introduction of the 7-Valent Pneumococcal Conjugate Vaccine into the Vaccination Calendar
Authors: Picazo J. , Ruiz-Contreras J. , Casado-Flores J. , Giangaspro E. , Del Castillo F. , Hernández-Sampelayo T. , Otheo E. , Balboa F. , Ríos E. , Méndez C. 
Published: November 2010

ABSTRACT

To assess invasive pneumococcal disease (IPD) clinical presentations and relationships with age and serotype in hospitalized children (<15 years) after PCV7 implementation in Madrid, Spain, a prospective 2-year (May 2007 to April 2009) laboratory-confirmed (culture and/or PCR) IPD surveillance study was performed (22 hospitals). All isolates (for serotyping) and culture-negative pleural/cerebrospinal fluids were sent to the reference laboratory for pneumolysin (
ply
) and autolysin (
lyt
) gene PCR analysis. A total of 330 IPDs were identified: 263 (79.7%) confirmed by culture and 67 (20.3%) confirmed by PCR. IPD distribution by age (months) was as follows: 23.6% (<12), 15.8% (12 to 23), 15.5% (24 to 35), 22.4% (36 to 59), and 22.7% (>59). Distribution by clinical presentation was as follows: 34.5% bacteremic pneumonia, 30.3% pediatric parapneumonic empyema (PPE), 13.6% meningitis, 13.3% primary bacteremia, and 8.2% others. Meningitis and primary bacteremia were the most frequent IPDs in children <12 months old, and bacteremic pneumonia and PPE were most frequent in those >36 months old. Frequencies of IPD-associated serotypes were as follows: 1, 26.1%; 19A, 18.8%; 5, 15.5%; 7F, 8.5%; 3, 3.9%; nontypeable/other 30 serotypes, 27.3%. Serotype 1 was linked to respiratory-associated IPD (38.6% in bacteremic pneumonia and 38.0% in PPE) and children of >36 months (51.4% for 36 to 59 months and 40.0% for >59 months), while serotype 19A was linked to nonrespiratory IPDs (31.1% in meningitis, 27.3% in primary bacteremia, and 51.9% in others) and children of <24 months (35.9% for children of <12 months and 36.5% for those 12 to 23 months old), with high nonsusceptibility rates for penicillin, cefotaxime, and erythromycin. After PCV7 implementation, non-PCV7 serotypes caused 95.5% of IPDs. The new 13-valent conjugate vaccine would provide 79.1% coverage of serotypes responsible for IPDs in this series.
Spoligotype Profile of
Mycobacterium tuberculosis
Complex Strains from HIV-Positive and -Negative Patients in Nigeria: a Comparative Analysis
Authors: Cadmus Simeon , Hill Véronique , van Soolingen Dick , Rastogi Nalin 
Published: November 2010

ABSTRACT

We ran a comparative analysis of all patients for whom a positive culture of
Mycobacterium tuberculosis
complex was available between April 2004 and October 2005 and whose HIV serology results were known, with spoligotyping results (
n
= 163) split into 49 HIV-positive patients and 114 HIV-negative patients. Spoligotype international type 373 (SIT373) (T1 lineage), which was highly prevalent among the HIV
+
patients, was totally absent from the HIV
−
population, suggesting that we had a specific clone affecting nearly 1/3 of all HIV-tuberculosis (TB)-coinfected patients. Among the LAM10-CAM sublineage strains, we had only a single strain of SIT403 among HIV
−
patients (0.88%), as opposed to 12.25% of the HIV
+
population (χ
2
= 10.77;
P
< 0.01), indicating a strong association between the strain and the HIV
+
population. The LAM10-CAM lineage spoligotype SIT61 was prevalent among the 2 subsets (37.72% in HIV
−
versus 12.24% in HIV
+
populations), though, with a significant difference between the 2 groups (χ
2
= 10.53;
P
< 0.01). However, there was no significant difference for SIT53 (T1 lineage) in the 2 subsets: 6.14 versus 8.2% (χ
2
= 0.22;
P
> 0.05). A total of 7/49, or 14.3%, other SITs among HIV
+
patients were not found among the HIV
−
patients. When added to the most prevalent SIT among HIV
+
patients (SIT373;
n
= 16), 23/49, or 47%, isolates among HIV-TB-coinfected patients were unique. We conclude that further studies should be carried out to investigate the evolution of these genotypes and others in the emergence of multidrug resistance and control of tuberculosis in Nigeria.
Human Parechovirus Infection in Children Hospitalized with Acute Gastroenteritis in Sri Lanka
Authors: Pham Ngan Thi Kim , Takanashi Sayaka , Tran Dinh Nguyen , Trinh Quang Duy , Abeysekera Chandra , Abeygunawardene Asiri , Khamrin Pattara , Okitsu Shoko , Shimizu Hiroyuki , Mizuguchi Masashi , Ushijima Hiroshi 
Published: November 2010

ABSTRACT
Of 362 fecal specimens collected from infants and children hospitalized with acute gastroenteritis in Sri Lanka from September 2005 to August 2006, 30 (8.3%) were positive for human parechovirus (HPeV). Six different HPeV genotypes, including HPeV1, -3, -4, -5, -10, and -11, were identified, of these, HPeV11 was reported for the first time.
The Tyro3 Receptor Kinase Axl Enhances Macropinocytosis of Zaire Ebolavirus
Authors: Hunt Catherine L. , Kolokoltsov Andrey A. , Davey Robert A. , Maury Wendy 
Published: November 2010

ABSTRACTAxl, a plasma membrane-associated Tyro3/Axl/Mer (TAM) family member, is necessary for optimal Zaire ebolavirus (ZEBOV) glycoprotein (GP)-dependent entry into some permissive cells but not others. To date, the role of Axl in virion entry is unknown. The focus of this study was to characterize entry pathways that are used for ZEBOV uptake in cells that require Axl for optimal transduction and to define the role of Axl in this process. Through the use of biochemical inhibitors, interfering RNA (RNAi), and dominant negative constructs, we demonstrate that ZEBOV-GP-dependent entry into these cells occurs through multiple uptake pathways, including both clathrin-dependent and caveola/lipid raft-mediated endocytosis. Other dynamin-dependent and -independent pathways such as macropinocytosis that mediate high-molecular-weight dextran uptake also stimulated ZEBOV-GP entry into these cells, and inhibitors that are known to block macropinocytosis inhibited both dextran uptake and ZEBOV infection. These findings provided strong evidence for the importance of this pathway in filovirus entry. Reduction of Axl expression by RNAi treatment resulted in decreased ZEBOV entry via macropinocytosis but had no effect on the clathrin-dependent or caveola/lipid raft-mediated endocytic mechanisms. Our findings demonstrate for the first time that Axl enhances macropinocytosis, thereby increasing productive ZEBOV entry.
On Invariant Tori of Nearly Integrable Hamiltonian Systems with Quasiperiodic Perturbation
Authors: Zhang Dongfeng , Cheng Rong 
Published: November 2010

AbstractWe are concerned with the persistence of frequency of invariant tori for analytic integrable Hamiltonian system with quasiperiodic perturbation. It is proved that if the unperturbed system satisfies the Rüssmann's nondegeneracy condition and has nonzero Brouwer's topological degree at some Diophantine frequency; the perturbed system satisfies the colinked nonresonant condition, then the invariant torus with this frequency persists under quasiperiodic perturbation.
Interleukin-6 Modulates Graft-versus-Host Responses after Experimental Allogeneic Bone Marrow Transplantation
Authors: Tawara Isao , Koyama Motoko , Liu Chen , Toubai Tomomi , Thomas Dafydd , Evers Rebecca , Chockley Peter , Nieves Evelyn , Sun Yaping , Lowler Kathleen P. , Malter Chelsea , Nishimoto Norihiro , Hill Geoffrey R. , Reddy Pavan 
Published: November 2010

Abstract
Purpose: The graft-versus-tumor (GVT) effect is a potent form of immunotherapy against many hematologic malignancies and some solid tumors. The beneficial GVT effect after allogeneic bone marrow transplantation (BMT) is tightly linked to its most significant complication, graft-versus-host disease (GVHD). The role of interleukin-6 (IL-6) after allogeneic BMT is not well understood. This study used a series of complementary knockout and antibody blockade strategies to analyze the impact of IL-6 in multiple clinically relevant murine models of GVHD and GVT.
Experimental Design: We examined the effect of the source of IL-6 by analyzing the role IL-6 deficiency in donor T cells, donor bone marrow or in host tissues. We confirmed and extended the relevance of IL-6 deficiency on GVHD and GVT by treating BMT recipients with anti-mouse IL-6 receptor (IL-6R), MR16-1.
Results: Deficiency of IL-6 in donor T cells led to prolongation of survival. Total inhibition of IL-6 with MR16-1 caused an even greater reduction in GVHD-induced mortality. The reduction in GVHD was independent of the direct effects on T effector cell expansion or donor regulatory T cells. GVT responses were preserved after treatment with MR16-1.
Conclusion: MR16-1 treatment reduced GVHD and preserved sufficient GVT. Tocilizumab, a humanized anti–IL-6R monoclonal antibody (mAb), is approved in several countries including the United States and European Union for the treatment of rheumatoid arthritis and other inflammatory diseases. Blockade of IL-6 with anti–IL-6R mAb therapy may be testable in clinical trials as an adjunct to prevent GVHD in BMT patients without a significant loss of GVT. Clin Cancer Res; 17(1); 77–88. ©2010 AACR.
Temporal updating during phonotaxis in male túngara frogs (Physalaemus pustulosus)
Authors: Baugh Alexander , Ryan Michael 
Published: November 2010

AbstractIn acoustically advertising anurans the male courtship call elicits species-typical responses from conspecifics – usually phonotactic approach and mate choice in gravid females and an evoked vocal response in adult males. Males in several species, however, are also known to perform phonotaxis, sometimes with the same acoustic preferences as females. Female túngara frogs are known to update their phonotactic approach as male advertisement signals change dynamically in attractiveness. Here we show that males also perform such temporal updating during phonotaxis in response to dynamic playbacks. While males exhibit slower phonotactic approaches than females, their responsiveness to dynamic changes in call complexity does not differ significantly compared to females. These results demonstrate that males are sensitive to the location of preferred call types on a moment-to-moment basis and suggest that similarities between male and female sexual behaviour in anurans might often be overlooked. We suggest that anuran phonotaxis is more widespread and serves different functions in reproductive females and males. Lastly, these temporal updating results suggest that male frogs are highly selective about site selection in a chorus.
Skeletochronological analysis of age structure in populations of four species of Malagasy poisonous frogs, genus Mantella
Authors: Jovanovic Olga , Vences Miguel 
Published: November 2010

AbstractAge structure of populations of four species of endemic Malagasy frogs of the genus Mantella (M. aurantiaca, M. baroni, M. bernhardi, M. madagascariensis) was examined by skeletochronology based on 96 specimens from nine different localities. In more than half of these (57%), no lines of arrested growth (LAGs) were found, and the number of LAGs recognized in the remaining specimens was mostly one, and probably two in three specimens. It is generally considered that each LAG corresponds to one year of life; our results therefore confirm that in Mantella populations almost all specimens are in their first or second year of life.
Cohabitation patterns of the San Marcos salamander (Eurycea nana)
Authors: Thaker Maria , Gabor Caitlin , Fries Joe , Epp Kristen 
Published: November 2010

AbstractSocial interactions of conspecifics are a function of complex relationships involving resource defense, antipredatory tactics, and mate acquisition. Consequently, individuals often associate non-randomly with conspecifics in their habitats, with spatial distributions of adults ranging from territorial spacing to aggregations. Site tenacity and cohabitation patterns have been well studied in many species of terrestrial salamander; however, less is understood about these behaviors in aquatic species. We examined the cohabitation patterns of intrasexual and intersexual pairs of the federally threatened, paedomorphic San Marcos salamander (Eurycea nana) under artificial shelters in a laboratory setting over a 20-day period. We found that intrasexual female pairs and intersexual pairs were found cohabiting more often than intrasexual male pairs. We also assessed site tenacity by examining shelter affinity and found that both males and females inhabited one of the two shelters more often than expected from random habitation, regardless of whether they were in intersexual or intrasexual pairings. Our results indicate that although both sexes of Eurycea nana exhibit site affinity, the sex of individuals is an important determinant of cohabitation patterns.
Estimation of Near-Ground Propagation Conditions Using Radar Ground Echo Coverage
Authors: Park Shinju , Fabry Frédéric 
Published: November 2010

Abstract
The vertical gradient of refractivity (dN/dh) determines the path of the radar beam; namely, the larger the negative values of the refractivity gradient, the more the beam bends toward the ground. The variability of the propagation conditions significantly affects the coverage of the ground echoes and, thus, the quality of the scanning radar measurements. The information about the vertical gradient of refractivity is usually obtained from radiosonde soundings whose use, however, is limited by their coarse temporal and spatial resolution. Because radar ground echo coverage provides clues about how severe the beam bending can be, we have investigated a method that uses radar observations to infer propagation conditions with better temporal resolution than the usual soundings.
Using the data collected during the International H2O Project (IHOP_2002), this simple method has shown some skill in capturing the propagation conditions similar to these estimated from soundings. However, the evaluation of the method has been challenging because of 1) the limited resolution of the conventional soundings in time and space, 2) the lack of other sources of data with which to compare the results, and 3) the ambiguity in the separation of ground from weather echoes.
Using a Stochastic Kinetic Energy Backscatter Scheme to Improve MOGREPS Probabilistic Forecast Skill
Authors: Tennant Warren J. , Shutts Glenn J. , Arribas Alberto , Thompson Simon A. 
Published: November 2010

Abstract
An improved stochastic kinetic energy backscatter scheme, version 2 (SKEB2) has been developed for the Met Office Global and Regional Ensemble Prediction System (MOGREPS). Wind increments at each model time step are derived from a streamfunction forcing pattern that is modulated by a locally diagnosed field of likely energy loss due to numerical smoothing and unrepresented convective sources of kinetic energy near the grid scale. The scheme has a positive impact on the root-mean-square error of the ensemble mean and spread of the ensemble. An improved growth rate of spread results in a better match with ensemble-mean forecast error at all forecast lead times, with a corresponding improvement in probabilistic forecast skill from a more realistic representation of model error. Other examples of positive impact include improved forecast blocking frequency and reduced forecast jumpiness. The paper describes the formulation of the SKEB2 and its assessment in various experiments.
Chemotherapy resistance in acute lymphoblastic leukemia requires hERG1 channels and is overcome by hERG1 blockers
Authors: Pillozzi Serena , Masselli Marika , De Lorenzo Emanuele , Accordi Benedetta , Cilia Emanuele , Crociani Olivia , Amedei Amedeo , Veltroni Marinella , D'Amico Massimo , Basso Giuseppe , Becchetti Andrea , Campana Dario , Arcangeli Annarosa 
Published: November 2010

AbstractBone marrow mesenchymal cells (MSCs) can protect leukemic cells from chemotherapy, thus increasing their survival rate. We studied the potential molecular mechanisms underlying this effect in acute lymphoblastic leukemia (ALL) cells. Coculture of ALL cells with MSCs induced on the lymphoblast plasma membrane the expression of a signaling complex formed by hERG1 (human ether-à-go-go-related gene 1) channels, the β1-integrin subunit, and the chemokine receptor CXC chemokine receptor-4. The assembly of such a protein complex activated both the extracellular signal-related kinase 1/2 (ERK1/2) and the phosphoinositide 3-kinase (PI3K)/Akt prosurvival signaling pathways. At the same time, ALL cells became markedly resistant to chemotherapy-induced apoptosis. hERG1 channel function appeared to be important for both the initiation of prosurvival signals and the development of drug resistance, because specific channel blockers decreased the protective effect of MSCs. NOD/SCID mice engrafted with ALL cells and treated with channel blockers showed reduced leukemic infiltration and had higher survival rates. Moreover, hERG1 blockade enhanced the therapeutic effect produced by corticosteroids. Our findings provide a rationale for clinical testing of hERG1 blockers in the context of antileukemic therapy for patients with ALL.
Vitamin D insufficiency and prognosis in chronic lymphocytic leukemia
Authors: Shanafelt Tait D. , Drake Matthew T. , Maurer Matthew J. , Allmer Cristine , Rabe Kari G. , Slager Susan L. , Weiner George J. , Call Timothy G. , Link Brian K. , Zent Clive S. , Kay Neil E. , Hanson Curtis A. , Witzig Thomas E. , Cerhan James R. 
Published: November 2010

AbstractVitamin D insufficiency is common globally and low levels are linked to higher cancer incidence. Although vitamin D insufficiency is related to inferior prognosis in some cancers, no data exist for chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL). We evaluated the relationship of 25(OH)D serum levels with time-to-treatment (TTT) and overall survival (OS) in newly diagnosed CLL patients participating in a prospective cohort study (discovery cohort) and a separate cohort of previously untreated patients participating in an observational study (confirmation cohort). Of 390 CLL patients in the discovery cohort, 119 (30.5%) were 25(OH)D insufficient. After a median follow-up of 3 years, TTT (hazard ratio[HR] = 1.66; P = .005) and OS (HR = 2.39; P = .01) were shorter for 25(OH)D-insufficient patients. In the validation cohort, 61 of 153 patients (39.9%) were 25(OH)D insufficient. After a median follow-up of 9.9 years, TTT (HR = 1.59; P = .05) and OS (HR 1.63; P = .06) were again shorter for 25(OH)D-insufficient patients. On pooled multivariable analysis of patients in both cohorts adjusting for age, sex, Rai stage, CD38 status, ZAP-70 status, immunoglobulin heavy chain variable (IGHV) gene mutation status, CD49d status, and cytogenetic abnormalities assessed by interphase fluorescent in situ hybridization testing, 25(OH)D insufficiency remained an independent predictor of TTT (HR = 1.47; P = .008), although the association with OS was not significant (HR = 1.47; P = .07). Vitamin D insufficiency is associated with inferior TTT and OS in CLL patients. Whether normalizing vitamin D levels in deficient CLL patients would improve outcome merits clinical testing.
Phase II trial of isotonic fluid resuscitation in Kenyan children with severe malnutrition and hypovolaemia
Authors: Akech Samuel O , Karisa Japhet , Nakamya Phellister , Boga Mwanamvua , Maitland Kathryn 
Published: November 2010

AbstractBackgroundChildren with severe malnutrition who develop shock have a high mortality. Contrary to contemporaneous paediatric practice, current guidelines recommend use of low dose hypotonic fluid resuscitation (half-strength Darrows/5% dextrose (HSD/5D). We evaluated the safety and efficacy of this guideline compared to resuscitation with a standard isotonic solution.MethodsA Phase II randomised controlled, safety and efficacy trial in Kenyan children aged over 6 months with severe malnutrition and shock including children with severe dehydration/shock and presumptive septic shock (non-diarrhoeal shock). Eligible children were randomised to HSD/5D or Ringer's Lactate (RL). A maximum of two boluses of 15 ml/kg of HSD/5D were given over two hours (as recommended by guidelines) while those randomised to RL received 10 ml/kg aliquots half hourly (maximum 40 ml/kg). Primary endpoint was resolution of shock at 8 and 24 hours. Secondary outcomes included resolution of acidosis, adverse events and mortality.Results61 children were enrolled: 41 had shock and severe dehydrating diarrhoea, 20 had presumptive septic shock; 69% had decompensated shock. By 8 hours response to volume resuscitation was poor with shock persisting in most children:-HSD/5D 15/22 (68%) and RL14/25 (52%), p = 0.39. Oliguria was more prevalent at 8 hours in the HSD/5D group, 9/22 (41%), compared to RL-3/25 (12%), p = 0.02. Mortality was high, HSD/5D-15/26(58%) and RL 13/29(45%); p = 0.42. Most deaths occurred within 48 hours of admission. Neither pulmonary oedema nor cardiogenic failure was detected.ConclusionsOutcome was universally poor characterised by persistence of shock, oliguria and high case fatality. Isotonic fluid was associated with modest improvement in shock and survival when compared to HSD/5D but inconclusive due to the limitations of design and effectiveness of either resuscitation strategy. Although isotonic fluid resuscitation did not result in cardiogenic heart failure, as previously feared, we conclude that the modest volumes used and rate of infusion were insufficient to promptly correct shock. The adverse performance of the recommended fluid resuscitation guideline for severe malnutrition should prompt clinical investigation of isotonic fluids for resuscitation of compensated shock, defining rate and volumes required to inform future guidelines.Trial RegistrationThe trial is registered as ISCRTN: 61146418
Retrospective seroepidemiology indicated that human enterovirus 71 and coxsackievirus A16 circulated wildly in central and southern China before large-scale outbreaks from 2008
Authors: Zhu Zhen , Zhu Shuangli , Guo Xuebin , Wang Jitao , Wang Dongyan , Yan Dongmei , Tan Xiaojuan , Tang Liuying , Zhu Hui , Yang Zhaohui , Jiang Xiaohong , Ji Yixin , Zhang Yong , Xu Wenbo 
Published: November 2010

Abstract

Background
Large nationwide outbreaks of hand, foot, and mouth disease (HFMD) occurred in China from 2008; most of the cases were in children under 5 years. This study aims to identify the situation of natural human enterovirus 71 (HEV71) and coxsackievirus A16 (CVA16) infections in children before 2008 in China.

Results
Retrospective seroepidemiologic studies of HEV71 and CVA16 were performed with 900 serum samples collected from children ≤5 years of age in 2005. The samples were collected from 6 different geographical areas (Anhui, Guangdong, Hunan, Xinjiang, Yunnan, and Heilongjiang provinces) in mainland China. Of the 900 samples, 288 were positive for HEV71; the total positive rate was 32.0% and the geometric mean titer (GMT) was 1:8.5. Guangdong (43.7% and 1:10.8), Xinjiang (45.4% and 1:11.1), and Yunnan (43.4% and 1:12.0) provinces had relatively high rates of infection, while Heilongjiang province (8.1% and 1:4.9) had the lowest rate of infection. On the other hand, 390 samples were positive for CVA16; the total positive rate was 43.4% and the GMT was 1:9.5. Anhui (62.2% and 1:16.0) and Hunan (61.1% and 1:23.1) had relatively high rates, while Heilongjiang (8.0% and 1:4.6) had the lowest rate. Although there is a geographical difference in HEV71 and CVA16 infections, low neutralizing antibody positive rate and titer of both viruses were found in all 6 provinces.

Conclusions
This report confirmed that HEV71 and CVA16 had wildly circulated in a couple provinces in China before the large-scale outbreaks from 2008. This finding also suggests that public health measures to control the spread of HEV71 and CVA16 should be devised according to the different regional characteristics.