bluuebunny/crossref_metadata_2025_split huggingface dataset:
Acute copper toxicity in the euryhaline copepod Acartia tonsa: implications for the development of an estuarine and marine biotic ligand model
Authors: Pinho Grasiela Lopes Leães , Bianchini Adalto 
Published: April 2010

Abstract
Copepods (Acartia tonsa) were exposed (48 h) to waterborne, diet-borne (non-Cu-equilibrated and Cu-equilibrated food), and waterborne plus diet-borne Cu in either the absence or the presence of food (diatom Thalassiosira weissflogii). Toxicity tests were run in different salinities (5, 15, and 30 ppt) together with measurements of physicochemical parameters and total and dissolved Cu concentrations in the experimental media. Results show that most of the toxic Cu fraction was in the dissolved phase. In general, Cu toxicity was higher in low (5 ppt) than in high salinity (30 ppt), regardless of the pathway of Cu exposure tested. In the absence of food, data clearly indicate that differences in waterborne Cu toxicity can be explained by changes in water chemistry. However, addition of food (either non-Cu-equilibrated or Cu-equilibrated) to the experimental media protected against acute Cu toxicity in salinities 5 and 15 ppt, suggesting that A. tonsa requires extra energy to cope with the stressful condition imposed by Cu exposure associated with the ionoregulatory requirements in low salinities. For diet-borne exposure, a very high Cu concentration was necessary to precontaminate the diatoms to a level resulting in copepod mortality. Therefore, availability of food exerted a more important positive impact in protecting against acute Cu toxicity than its potential negative impact via contamination resulting in toxicity. Findings indicate the need for incorporation of both salinity and food in a future biotic ligand model (BLM) version for Cu in estuarine and marine waters. In this context, the euryhaline copepod A. tonsa would be a suitable model species with which to perform experiments to validate and calibrate any future saltwater BLM. Environ. Toxicol. Chem. 2010; 29:1834–1840. © 2010 SETAC
Functional behavior and reproduction in androgenic sex reversed zebrafish (Danio rerio)
Authors: Larsen Mia G. , Baatrup Erik 
Published: April 2010

AbstractEndocrine-disrupting chemicals released into natural watercourses may cause biased sex ratios by sex reversal in fish populations. The present study investigated the androgenic sex reversal of zebrafish (Danio rerio) exposed to the androgenic compound 17β-trenbolone (TB) and whether sex-changed females would revert to the female phenotype after cessation of TB exposure. 17β-Trenbolone is a metabolite of trenbolone acetate, an anabolic steroid used as a growth promoter in beef cattle. 17β-Trenbolone in runoff from cattle feedlots may reach concentrations that affect fish sexual development. Zebrafish were exposed to a concentration of 20 ng/L TB in a flow-through system for five months from egg until sexual maturity. This resulted in an all-male population. It was further found that all these phenotypic males displayed normal male courtship behavior and were able to reproduce successfully, implying that the sex reversal was complete and functional. None of the phenotypic males developed into females after six months in clean water, demonstrating that androgenic sex reversal of zebrafish is irreversible. Environ. Toxicol. Chem. 2010; 29:1828–1833. © 2010 SETAC
Occurrence and fate of micropollutants in the Vidy Bay of Lake Geneva, Switzerland. Part II: Micropollutant removal between wastewater and raw drinking water
Authors: Morasch Barbara , Bonvin Florence , Reiser Hans , Grandjean Dominique , de Alencastro Luiz Felippe , Perazzolo Chiara , Chèvre Nathalie , Kohn Tamar 
Published: April 2010

Abstract
The occurrence and removal of 58 pharmaceuticals, endocrine disruptors, corrosion inhibitors, biocides, and pesticides, were assessed in the wastewater treatment plant (WWTP) of the city of Lausanne, Switzerland, as well as in the effluent-receiving water body, the Vidy Bay of Lake Geneva. An analytical screening method to simultaneously measure all of the 58 micropollutants was developed based on ultra performance liquid chromatography coupled to a tandem mass spectrometer (UPLC-MS/MS). The selection of pharmaceuticals was primarily based on a prioritization study, which designated them as environmentally relevant for the Lake Geneva region. Except for the endocrine disruptor 17α-ethinylestradiol, all substances were detected in 24-h composite samples of wastewater entering the WWTP or in the treated effluent. Of these compounds, 40% were also detected in raw drinking water, pumped from the lake 3 km downstream of the WWTP. The contributions of dilution and degradation to micropollutant elimination between the WWTP outlet and the raw drinking water intake were established in different model scenarios using hypothetical residence times of the wastewater in Vidy Bay of 1, 4, or 90 d. Concentration decrease due to processes other than dilution was observed for diclofenac, beta-blockers, several antibiotics, corrosion inhibitors, and pesticides. Measured environmental concentrations (MECs) of pharmaceuticals were compared to the predicted environmental concentrations (PECs) determined in the prioritization study and agreed within one order of magnitude, but MECs were typically greater than the corresponding PECs. Predicted no-effect concentrations of the analgesic paracetamol, and the two antibiotics ciprofloxacin and sulfamethoxazole, were exceeded in raw drinking water samples and therefore present a potential risk to the ecosystem. Environ. Toxicol. Chem. 2010; 29:1658–1668. © 2010 SETAC
On the temporal stability and accuracy of coupled problems with reference to fluid–structure interaction
Authors: Joosten M. M. , Dettmer W. G. , Perić D. 
Published: April 2010

AbstractThis work investigates the effect of employing different time integration schemes in the sub‐domains of a coupled problem, such as fluid–structure interaction. On the basis of a one‐dimensional model problem and the two versions of the generalized‐α method developed for first‐ and second‐order systems, it is shown that the overall problem is likely to be less stable and less accurate than the individual sub‐problems unless special measures are taken. The benchmark problem of the oscillating flexible beam is used to demonstrate that these findings also apply to full computational fluid–structure interaction. Copyright © 2010 John Wiley & Sons, Ltd.
Test of three methods to detect the overbank flow from water level time‐series analysis
Authors: Navratil O. , Albert M. B. , Breil P. 
Published: April 2010

AbstractIn this study, we propose to identify morphological and hydraulic characteristics related to overbank flows in the water level time‐series available at many gauged stations. The results obtained at 13 river stations (the catchment sizes vary from 10 to 1700 km2 with contrasted geology, land use and rainfalls regime) show that overflow mechanisms at the river‐reach scale can be systematically identified in the water level frequency distribution estimated with the peaks‐over‐threshold (POT) method. A first level (Lts1) was in the range of the incipient flooding onto the floodplain. Even if the definition of this level is variable in terms of flooded area at the reach scale, this method can be useful in providing a first estimation of the bankfull level for many gauged stations, without complex and costly field surveys. A second level (Lts2) was systematically detected on average 38 cm above the topographic flat floodplain elevation. The Lts2 inflection in the water‐level frequency distribution is assumed to reflect a composite effect of catchment hydrology and local hydraulics and channel geometry, without possibility to make a clear distinction between both processes at the moment. The local or reach scale effect would probably play an essential role in the frequency distribution as flood attenuation at catchment scale may explain the inflection Lts2 at only three sites. In light of the knowledge acquired in laboratory flumes with compound channels, most of the time Lts2 level would correspond to the level of resumption of flow both in the main channel and the floodplain. Once this method is validated in various physiographic contexts, it should apply to many hydrometric stations for both synchronic (e.g. regional analysis) and diachronic analysis (e.g. evolution over time of the bankfull discharge) to evaluate anthropic impacts on river morphology. Copyright © 2010 John Wiley & Sons, Ltd.
Past and projected future changes in snowpack and soil frost at the Hubbard Brook Experimental Forest, New Hampshire, USA
Authors: Campbell John L. , Ollinger Scott V. , Flerchinger Gerald N. , Wicklein Haley , Hayhoe Katharine , Bailey Amey S. 
Published: April 2010

AbstractLong‐term data from the Hubbard Brook Experimental Forest in New Hampshire show that air temperature has increased by about 1 °C over the last half century. The warmer climate has caused significant declines in snow depth, snow water equivalent and snow cover duration. Paradoxically, it has been suggested that warmer air temperatures may result in colder soils and more soil frost, as warming leads to a reduction in snow cover insulating soils during winter. Hubbard Brook has one of the longest records of direct field measurements of soil frost in the United States. Historical records show no long‐term trends in maximum annual frost depth, which is possibly confounded by high interannual variability and infrequency of major soil frost events. As a complement to field measurements, soil frost can be modelled reliably using knowledge of the physics of energy and water transfer. We simulated soil freezing and thawing to the year 2100 using a soil energy and water balance model driven by statistically downscaled climate change projections from three atmosphere‐ocean general circulation models under two emission scenarios. Results indicated no major changes in maximum annual frost depth and only a slight increase in number of freeze–thaw events. The most important change suggested by the model is a decline in the number of days with soil frost, stemming from a concurrent decline in the number of snow‐covered days. This shortening of the frost‐covered period has important implications for forest ecosystem processes such as tree phenology and growth, hydrological flowpaths during winter, and biogeochemical processes in soil. Published in 2010 by John Wiley & Sons, Ltd.
Interception losses from Miscanthus at a site in south‐east England—an application of the Gash model
Authors: Finch J. W. , Riche A. B. 
Published: April 2010

AbstractConcern has been expressed that Miscanthus x giganteus, a dedicated biomass crop, may have a high water use, with implications for its economic yield and impacts on water resources. There is particular uncertainty about one component of the water use, the interception loss. Measurements of the interception loss were made in a plot of the crop at a site in south‐east England, during 1997/1998 and 1998/1999. The measured interception losses were 25 and 24% of gross rainfall, respectively. Winter interception losses are relatively high, which is attributed to the slow rate of leaf loss. A Monte Carlo procedure was used to optimize three of the parameters of the Gash interception model on the 1997/1998 data. The simulated values had an uncertainty of 1·1 mm per storm in 1997/1998 and 2·9 mm per storm in 1998/1999. The model was also used to investigate the potential effect of the evaporation rate being overestimated due to the measurements being made in an experimental plot. This showed that the interception losses might be reduced to 21 and 18% in field scale plantations. A consideration of the relative interception rate demonstrated that the crop behaved more like a forest, in terms of the interception losses, during the winter months. © Crown Copyright 2010. Reproduced with the permission of Her Majesty's Stationery Office. Published by John Wiley & Sons, Ltd.
Molecular approaches to examine the phosphorylation state of the C type natriuretic peptide receptor
Authors: Alli Abdel A. , Gower William R. 
Published: April 2010

AbstractThe intracellular domain of the C type natriuretic peptide receptor (NPRC) contains one threonine and several serine residues where phosphorylation is thought to occur. Several phosphorylation consensus sequences for various kinases have been identified within the intracellular domain of NPRC, but the exact residues that are phosphorylated and the specific kinases responsible for their phosphorylation have not been thoroughly defined. Here we introduce a recombinant GST fusion protein and a rat gastric mucosa (RGM1) cell line as molecular tools to study the phosphorylation state of NPRC in vitro and in vivo, respectively. We utilize a previously characterized polyclonal antibody against NPRC to probe for total NPRC protein and various phosphospecific and substrate motif antibodies to probe for phosphorylation of NPRC. Phosphoprotein staining reagents were used with a phosphoprotein control set to detect phosphorylation of NPRC at serine and threonine residues. Recombinant GST‐NPRC fusion protein was phosphorylated in vitro by RGM1 lysate in the presence of adenosine‐5'‐triphosphate (ATP). Western blot analysis using a monoclonal phospho‐Thr antibody, which exclusively detects phosphorylated threonine residues, and does not cross‐react with phosphorylated serine residues revealed NPRC immunoprecipitated from RGM1 lysate is phosphorylated on a threonine residue. Global analysis of the entire rat NPRC sequence using a protein kinase A (PKA) prediction algorithm, identified five putative PKA phosphorylation sites containing a serine residue and one containing a threonine residue, Thr 505. Taken together, the data presented here suggest that rat NPRC is a substrate for PKA and Thr 505 located within the intracellular domain of NPRC is a likely candidate site for the phosphorylation. J. Cell. Biochem. 110: 985–994, 2010. © 2010 Wiley‐Liss, Inc.
A 15 000‐year record of climate change in northern New Mexico, USA, inferred from isotopic and elemental contents of bog sediments
Authors: Cisneros‐Dozal Luz M. , Heikoop Jeffrey M. , Fessenden Julianna , Anderson R. Scott , Meyers Philip A. , Allen Craig D. , Hess Marcey , Larson Toti , Perkins George , Rearick Michael 
Published: April 2010

AbstractElemental (C, N, Pb) and isotopic (δ13C, δ15N) measurements of cored sediment from a small bog in northern New Mexico reveal changes in climate during the Late Pleistocene and Holocene. Abrupt increases in Pb concentration and δ13C values ca. 14 420 cal. YBP indicate significant runoff to the shallow lake that existed at that time. Weathering and transport of local volcanic rocks resulted in the delivery of Pb‐bearing minerals to the basin, while a 13C‐enriched terrestrial vegetation source increased the δ13C values of the sedimentary material. Wet conditions developed over a 300 a period and lasted for a few hundred years. The Younger Dryas period (ca. 12 700–11 500 cal. YBP) caused a reduction in terrestrial productivity reflected in decreasing C/N values, δ15N values consistently greater than 0‰ and low organic content. By contrast, aquatic productivity increased during the second half of this period, evidenced by increasing δ13C values at the time of highest abundance of algae. Dry conditions ca. 8 000–6 000 cal. YBP were characterised by low organic carbon content and high Pb concentrations, the latter suggesting enhanced erosion and aeolian transport of volcanic rock. The range in δ13C, δ15N and C/N values in the sedimentary record fall within the range of modern plants, except during the periods of runoff and drought. The sedimentary record provides evidence of natural climate variability in northern New Mexico, including short‐ (multi‐centennial) and long‐(millennial) term episodes during the Late Pleistocene and Holocene. Copyright © 2010 John Wiley & Sons, Ltd.
Early to mid‐Holocene climate change at Lago dell'Accesa (central Italy): climate signal or anthropogenic bias?
Authors: Finsinger Walter , Colombaroli Daniele , De Beaulieu Jacques‐Louis , Valsecchi Verushka , Vannière Boris , Vescovi Elisa , Chapron Emmanuel , Lotter André F. , Magny Michel , Tinner Willy 
Published: April 2010

AbstractDespite the high potential of pollen records for climate reconstruction, pollen–climate relationships may be biased due to past and present human activities on the landscape. We use (i) transfer functions based on modern pollen–climate relationships to infer seasonal temperature and summer precipitation for the period 11 500–4500 cal. a BP and (ii) lake‐level change records based on different sedimentary proxies in multiple cores that are mainly indicative for summer hydrology at Lago dell'Accesa (central Italy). Quantitative reconstructions indicate lowest summer precipitation during two phases (8500–7700 cal. a BP and after 6000 cal. a BP) and a gradual winter temperature increase from 11 500 to ca. 8000 cal. a BP. Lowest summer precipitation was reconstructed during these phases characterised by vegetation shifts from open forests dominated by summergreen oaks (Quercus) to forests dominated by evergreen oaks (Quercus ilex), which are at present most abundant where summer drought is stronger. Similarly, the lake‐level record indicates two long‐lasting low summer precipitation phases (8800–7700 and 6400–4400 cal. a BP) that were interrupted by short‐term high summer precipitation events. Based on the broad agreement between the pollen‐inferred summer precipitation and the low‐frequency lake‐level changes, we suggest that the duration of the high summer precipitation events may have been too short to maintain drought‐sensitive trees, which may have been affected by high mortality rates when summer dry conditions returned. Although past and modern pollen–climate relationships may very likely have been affected by human activities since the Neolithic (i.e. when exploitation of the landscape started), we reject the hypothesis of a significant anthropogenic bias in the pollen‐based climate reconstruction. In addition, we suggest that pollen‐based and lake‐level reconstructions may have different inherent abilities of capturing high‐ and low‐frequency precipitation signals. Copyright © 2010 John Wiley & Sons, Ltd.
A comparative study of surface‐enhanced Raman scattering from silver‐coated anodic aluminum oxide and porous silicon
Authors: Terekhov S. N. , Mojzes P. , Kachan S. M. , Mukhurov N. I. , Zhvavyi S. P. , Panarin A. Yu. , Khodasevich I. A. , Orlovich V. A. , Thorel A. , Grillon F. , Turpin P.‐Y. 
Published: April 2010

AbstractThree types of Ag‐coated arrays from porous anodic aluminum oxide (AAO) were prepared and studied as substrates for surface‐enhanced Raman scattering (SERS). They were compared with Ag‐coated porous silicon (PSi) samples. AAO‐based substrates were prepared by the vapor deposition of silver directly onto the surface of porous AAO with different morphologies of the pores, whereas SERS‐active island films on the PSi were prepared by immersion plating. The resulting metallic nanostructures were characterized by UV‐vis absorption spectroscopy and scanning electron microscopy (SEM). Thermal evaporation leads to the formation of granular arrays of Ag nanoparticles on the surface of AAO. SERS activity of the substrates was tested using water‐soluble cationic Zn(II)‐tetrakis (4‐N‐methylpyridyl) porphyrin (ZnTMPyP4) as a probe molecule. The results indicate that all AAO‐based substrates studied here exhibit some degree of SERS activity. Noteworthy, for excitation at 532 nm, signals from AAO‐based substrates were comparable with those from the PSi‐based ones, whereas for 441.6 nm excitation they were about twice higher. The strongest SERS‐enhancement at 441.6 nm excitationwas provided by the AAO substrates with silver deposited on the monolith (originally nonporous) side of AAO. Preferential SERS‐enhancement of the bands ascribed to the vibrations of the N‐methylpyridinium group of ZnTMPyP4 when going to blue excitation was found. Copyright © 2010 John Wiley & Sons, Ltd.
Rotational CARS N2 thermometry: validation experiments for the influence of nitrogen spectral line broadening by hydrogen
Authors: Bohlin Alexis , Vestin Fredrik , Bonamy Jeanine , Joubert Pierre , Bengtsson Per‐Erik 
Published: April 2010

AbstractRotational coherent anti‐Stokes Raman spectroscopy (CARS) in fuel‐rich hydrocarbon flames, with a large content of hydrogen in the product gases (∼20%), has in previous work shown that evaluated temperatures are raised several tens of Kelvin by taking newly derived N2H2 Raman line widths into account. To validate these results, in this work calibrated temperature measurements at around 300, 500 and 700 K were performed in a cell with binary gas mixtures of nitrogen and hydrogen. The temperature evaluation was made with respect to Raman line widths either from self‐broadened nitrogen only, N2N2 [energy‐corrected‐sudden (ECS)], or by also taking nitrogen broadened by hydrogen, N2H2 [Robert–Bonamy (RB)], Raman line widths into account. With increased amount of hydrogen in the cell at constant temperature, the evaluated CARS temperatures were clearly lowered with the use of Raman line widths from self‐broadened nitrogen only, and the case with inclusion of N2H2 Raman line widths was more successful. The difference in evaluated temperatures between the two different sets increases approximately linearly, reaching 20 K (at T ∼ 300 K), 43 K (at T = 500 K) and 61 K (at T = 700 K) at the highest hydrogen concentration (90%). The results from this work further emphasize the importance of using adequate Raman line widths for accurate rotational CARS thermometry. Copyright © 2010 John Wiley & Sons, Ltd.
Ultraviolet Raman scattering in ZnO nanowires: quasimode mixing and temperature effects
Authors: Alarcón‐Lladó E. , Ibáñez J. , Cuscó R. , Artús L. , Prades J. D. , Estradé S. , Morante J. R. 
Published: April 2010

AbstractWe use near‐resonance Raman scattering to investigate zinc oxide (ZnO) nanowires grown by chemical vapor deposition on Si substrates. We discuss the role of quasimode mixing on the wavenumber of the longitudinal optical (LO) bands, and we perform Raman measurements with different excitation powers to investigate possible laser heating effects. We find that in the Raman spectra of as‐deposited nanowires grown along the c‐axis of wurtzite, the LO bands are located slightly below the E1(LO) mode of bulk ZnO. We perform a calculation of the expected LO wavenumber in an ensemble of randomly oriented nanowires. Our analysis shows that light refraction, together with the orientation‐dependent cross‐section of the nanowires for the incoming light, counterbalances quasimode mixing effects in the as‐grown product, giving rise to LO bands that are barely redshifted relative to the E1(LO) mode. In the case of ZnO nanowires that have been mechanically removed (scratched) and subsequently deposited onto separate Si substrates, we observe clear laser‐induced heating. Temperature effects account well for the Raman wavenumber shifts displayed by the LO bands in the Raman spectra of the scratched nanowires. Copyright © 2010 John Wiley & Sons, Ltd.
Pigment identification of a rare 18th century wallpaper from Buffon library
Authors: Colomban Philippe 
Published: April 2010

AbstractA rare 18th century wallpaper depicting a basket of flowers has been recently discovered in the building where Buffon, the famous French Naturalist, had established his Library and written a large part of its ‘Histoire Naturelle’. The wallpaper fragments were analysed and some pigments identified: gypsum, calcite, carbon black, vermilion and lapis lazuli; a blue/green organic compound was seemingly used to dye the paper. Copyright © 2010 John Wiley & Sons, Ltd.