bluuebunny/crossref_metadata_2025_split huggingface dataset:
Towards Evidence‐based Management: Creating an Informative Database of Nursing‐Sensitive Indicators
Authors: Patrician Patricia A. , Loan Lori , McCarthy Mary , Brosch Laura R. , Davey Kimberly S. 
Published: October 2010

Abstract Purpose: The purpose of this paper is to describe the creation, evolution, and implementation of a database of nursing‐sensitive and potentially nursing‐sensitive indicators, the Military Nursing Outcomes Database (MilNOD). It discusses data quality, utility, and lessons learned. Design/Methods: Prospective data collected each shift include direct staff hours by levels (i.e., registered nurse, other licensed and unlicensed providers), staff categories (i.e., military, civilian, contract, and reservist), patient census, acuity, and admissions, discharges, and transfers. Retrospective adverse event data (falls, medication errors, and needle‐stick injuries) were collected from existing records. Annual patient satisfaction, nurse work environment, and pressure ulcer and restraint prevalence surveys were conducted. Findings and Conclusions: The MilNOD contains shift level data from 56 units in 13 military hospitals and is used to target areas for managerial and clinical performance improvement. This methodology can be modified for use in other healthcare systems. Clinical Relevance: As standard tools for evidence‐based management, databases such as MilNOD allow nurse leaders to track the status of nursing and adverse events in their facilities.
Vitamin K2 modulates differentiation and apoptosis of both myeloid and erythroid lineages
Authors: Sada Eriko , Abe Yasunobu , Ohba Rie , Tachikawa Yoshimichi , Nagasawa Eriko , Shiratsuchi Motoaki , Takayanagi Ryoichi 
Published: October 2010

AbstractVitamin K2 (VK2) can improve cytopenia in some patients with myelodysplastic syndrome (MDS). Although it is well known that VK2 induces differentiation and apoptosis in acute myeloid leukemia (AML) cell lines, little is known about its effect on normal hematopoietic progenitors. The effects of VK2 on primary myeloid and erythroid progenitors were examined. Mobilized CD34‐positive cells from peripheral blood were used for the examination of myeloid lineage cells, and erythroid progenitors purified from peripheral blood were used for erythroid lineage cells. VK2 upregulated the expressions of myeloid markers CD11b and CD14, and increased the mRNA expression levels of CCAAT/enhancer binding protein‐α (C/EBPα) and PU.1 in myeloid progenitors. In erythroid progenitors, VK2 did not show a significant effect on differentiation. However, VK2 exhibited an anti‐apoptotic effect on erythroid progenitors under erythropoietin depletion. This anti‐apoptotic effect was restricted to normal erythroid progenitors and was not shown in erythroleukemic cell line AS‐E2. Steroid and xenobiotic receptor (SXR), which was recently identified as a receptor of VK2, was expressed on myeloid progenitors, and the SXR agonist rifampicin (RIF) also upregulated CD11b and CD14 expressions on myeloid progenitors. These results indicate that SXR is involved in the effect of VK2 on myeloid progenitors. The major effect of VK2 on myeloid progenitors was promoting differentiation, whereas its anti‐apoptotic effect seemed to be dominant in erythroid progenitors. Although the detailed mechanism of VK2’s effect on differentiation or apoptosis of hematopoietic progenitors remains unknown, the effect of VK2 therapy in patients with MDS could be partly explained by these mechanisms.
CXCR7: a new SDF‐1‐binding receptor in contrast to normal CD34+ progenitors is functional and is expressed at higher level in human malignant hematopoietic cells
Authors: Tarnowski Maciej , Liu Rui , Wysoczynski Marcin , Ratajczak Janina , Kucia Magda , Ratajczak Mariusz Z. 
Published: October 2010

AbstractCXCR7 was identified as another stromal‐derived factor‐1 (SDF‐1)‐binding receptor that also binds the interferon‐inducible T‐cell chemoattractant (I‐TAC), and we became interested in its potential role in migration/adhesion of normal hematopoietic stem/progenitor cells (HSPCs) as well as selected leukemia cell lines. To address this normal human bone marrow‐, umbilical cord blood‐, and mobilized peripheral blood‐derived cells as well as 16 selected human leukemic cell lines were phenotyped for CXCR7 expression. The expression of CXCR7 in hematopoietic cell lines was analyzed at transcriptional level. The biologic significance of CXCR7 expression was subsequently tested in signal transduction studies as well as in in vitro proliferation and chemotactic assays. We noted that CXCR7 is expressed at very low levels (approximately 3–6%) in normal human CD34+ cells isolated from bone marrow, umbilical cord blood, and mobilized peripheral blood. More importantly, when we employed I‐TAC, which activates CXCR7, but not CXCR4, we did not observe any chemotactic responsiveness in human clonogenic progenitors. As expected, I‐TAC also did not affect clonogenic growth of human CD34+ cells. In contrast, functional CXCR7, whose expression is regulated in an NF‐κΒ‐dependent manner, as we report here, is highly expressed in several human myeloid malignant cell lines. I‐TAC‐induced activation of CXCR7 in human hematopoietic cell lines leads to phosphorylation of MAPKp42/44 and AKT, and enhanced cell adhesion and slightly cell migration. In conclusion, CXCR7 is expressed at very low level on normal human HSPCs and does not play a direct role in their proliferation or slightly cell migration; however, in contrast, it is involved in trafficking/adhesion of human leukemic cells.
Parental support for newborn screening for cystic fibrosis
Authors: de Monestrol Isabelle , Brucefors Agneta Bergsten , Sjöberg Birgitta , Hjelte Lena 
Published: October 2010

AbstractAim:  To describe the attitudes among parents towards including cystic fibrosis (CF) in the newborn screening programme and towards the potential knowledge of their own carrier status.Methods:  A questionnaire with three to five response categories and an information leaflet was posted to 143 CF parents, 3 matched diabetes and 3 matched population parents, the response rate being 85%, 74% and 70%, respectively. Comparisons between groups were made with statistical tests for independent groups.Results:  Eighty‐six percent of CF, 70% of diabetes and 77% of population parents were in favour of newborn screening for CF, 47%, 45% and 50%, respectively, wished to know their CF carrier status. The parental attitude was independent of the age of the child, as well as delay of diagnosis and well‐being of the CF child at the time of diagnosis. Sixty percent of the CF parents experienced the diagnosis as delayed.Conclusion:  Parents in Sweden support CF newborn screening. Half of the parents wanted to know their CF carrier status.
Parental perception of overweight and underweight in children and adolescents
Authors: Júlíusson Pétur B , Roelants Mathieu , Markestad Trond , Bjerknes Robert 
Published: October 2010

AbstractAims:  To compare the parental perception of overweight and underweight in their children to objective criteria, based on body mass index (BMI), waist circumference and triceps skinfold thickness, and to explore the effects of potential determinants.Methods:  Logistic regression of anthropometric measurements, socio‐demographic characteristics and self‐reported parental height and weight on the parental perception of their child’s weight status in 3770 children aged 2–19.Results:  Seventy per cent of overweight/obese children and 40.8% of underweight children were perceived having normal weight by parents. In 2‐ to 5‐year‐old overweight children, 91.2% were considered to have normal weight. For a given BMI, primary school age children, adolescents and girls had a higher probability to be assigned as overweight, whereas adolescents and girls had a lower probability to be assigned as underweight. Overweight parents more readily assigned their children as underweight, but there was no effect of parental educational level or parental underweight.Conclusion:  Parental ability to recognize overweight or underweight in their offspring was generally poor. The findings emphasize the need for objective criteria based on physical measurements in the routine follow up of children, as parental ability to recognize weight problems in their children is nonreliable.
Insights into Strata Use of Forest Animals Using the ‘Canopy Effect’
Authors: Voigt Christian C. 
Published: October 2010

ABSTRACTStable carbon isotope ratios (δ13C) of plants increase linearly from ground to canopy. Accordingly, I used δ13C for estimating strata use of fig‐eating bats. Data suggest that, overall, bats commuted at lower but fed at higher forest strata, and that small bats foraged at lower forest strata than large bats.
Low‐Dose Statin Therapy Improves Endothelial Function in Type 2 Diabetic Patients With Normal Serum Total Cholesterol: A Randomized Placebo‐Controlled Study
Authors: Adel Amr , Abdel‐Salam Zainab , Nammas Wail 
Published: October 2010

J Clin Hypertens (Greenwich). 2010;12:820‐825. © 2010 Wiley Periodicals, Inc.The authors sought to explore the effect of low‐dose atorvastatin on endothelial function in normocholesterolemic patients with type 2 diabetes mellitus without evidence of coronary disease. Sixty patients with type 2 diabetes mellitus, normal serum cholesterol, and normal exercise test results were enrolled. Initial assessment was performed by measurement of the brachial artery diameter and flow velocity both at baseline and after induced hyperemia. Patients were randomly assigned to receive either atorvastatin 10 mg daily for 4 weeks (atorvastatin group=30 patients) or matched placebo for the same period (placebo group=30 patients). Follow‐up assessment of the brachial artery diameter and flow velocity (both baseline and hyperemic) was performed after 4 weeks. Initially, no significant difference was found between the two groups regarding brachial artery diameter or flow velocity, both at baseline and at peak hyperemia (P>.05 for all). At follow‐up, there was a significantly higher flow velocity at baseline (P<.05) and a significantly higher percent increase of brachial artery diameter (from baseline to peak hyperemia) in the atorvastatin group (P<.05). In patients with type 2 diabetes mellitus and normal serum cholesterol without evidence of coronary disease, low‐dose atorvastatin improves endothelial function.
Proline metabolism in the moderately halophilic bacterium Halobacillus halophilus: differential regulation of isogenes in proline utilization
Authors: Köcher Saskia , Tausendschön Michaela , Thompson Melanie , Saum Stephan H. , Müller Volker 
Published: October 2010

SummaryThe amino acid proline is not only synthesized as a compatible solute but also used as a carbon and energy source by the moderately halophilic bacterium Halobacillus halophilus. Growth on proline was not affected by the salinity of the medium. Proline was degraded by proline dehydrogenase (ProDH) and Δ1‐pyrroline‐5‐carboxylate dehydrogenase (P5CDH) to glutamate via Δ1‐pyrroline‐5‐carboxylate. The basic biochemical parameters for ProDH and P5CDH activities were obtained for both in cell free extracts. The encoding genes were identified. H. halophilus has two isogenes each for prodh and p5cdh. prodh2 and p5cdh2 form an operon (put operon) whose mRNA is highly abundant in proline‐grown cells. Expression of the put operon was also upregulated by salinity and late growth phase in glucose‐grown cells. Similarly, ProDH and P5CDH activities increased in late exponential growth phase. This observation is in line with the previous notion that the compatible solute proline is degraded in stationary phase (in glucose grown cultures).
Hyperchloremic acidosis during plasma expansion
Authors: TAVERNIER BENOÎT , FAIVRE SÉBASTIEN , BOURDON CAROLINE 
Published: October 2010

SUMMARYMany crystalloid and colloid plasma expanders can induce ‘hyperchloremic’ metabolic acidosis. This finding, first reported over 50 years ago, has received renewed interest following the recent availability of ‘balanced’ solutions. Several questions must be addressed and answered before making recommendations with regard to the use of these solutions. Do acidosis and hyperchloremia always go hand in hand? What is the clinical impact of hyperchloremic acidosis? Are all ‘balanced’ plasma expanders equivalent? Should isotonic saline‐based plasma expanders be abandoned? Several elements of reflection are summarized in this brief review. On the basis on the currently available literature, it is not objectively possible to propose clear‐cut guidelines.
Finite Element Algorithm for Frictionless Contact of Porous Permeable
Media Under Finite Deformation and Sliding
Authors: Ateshian Gerard A. , Maas Steve , Weiss Jeffrey A. 
Published: October 2010

Abstract
This study formulates and implements a finite element contact algorithm for solid-fluid (biphasic) mixtures, accommodating both finite deformation and sliding. The finite element source code is made available to the general public. The algorithm uses a penalty method regularized with an augmented Lagrangian method to enforce the continuity of contact traction and normal component of fluid flux across the contact interface. The formulation addresses the need to automatically enforce free-draining conditions outside of the contact interface. The accuracy of the implementation is verified using contact problems, for which exact solutions are obtained by alternative analyses. Illustrations are also provided that demonstrate large deformations and sliding under configurations relevant to biomechanical applications such as articular contact. This study addresses an important computational need in the biomechanics of porous-permeable soft tissues. Placing the source code in the public domain provides a useful resource to the biomechanics community.
CALCULATION OF VOLUME AS FUNCTIONS OF TEMPERATURE AND PRESSURE IN ICE I CLOSE TO THE MELTING POINT
Authors: KILIT E. , YURTSEVEN H. 
Published: October 2010

We calculate in this study the volume of ice I as functions of temperature and pressure close to the melting point by analyzing the experimental data for the thermal expansivity. Using an approximate relation, the temperature dependence of the volume is calculated at 202.4 MPa from the thermal expansivity of ice I. The pressure dependence of the volume is also calculated at 252.3 K from the isothermal compressibility of ice I close to the melting point. The volume calculated here as functions of temperature and pressure shows critical behavior close to the melting point in ice I, which can be tested by the experimental measurements.
On the interaction of clocks, power, and synchronization in duty-cycled embedded sensor nodes
Authors: Schmid Thomas , Shea Roy , Charbiwala Zainul , Friedman Jonathan , Srivastava Mani B. , Cho Young H. 
Published: October 2010

The efficiency of the time synchronization service in wireless sensor networks is tightly connected to the design of the radio, the quality of the clocking hardware, and the synchronization algorithm employed. While improvements can be made on all levels of the system, over the last few years most work has focused on the algorithmic level to minimize message exchange and in radio architectures to provide accurate time-stamping mechanisms. Surprisingly, the influences of the underlying clock system and its impact on the overall synchronization accuracy has largely been unstudied.
In this work, we investigate the impact of the clocking subsystem on the time synchronization service and address, in particular, the influence of changes in environmental temperature on clock drift in highly duty-cycled wireless sensor nodes. We also develop formulas that help the system architect choose the optimal resynchronization period to achieve a given synchronization accuracy. We find that the synchronization accuracy has a two region behavior. In the first region, the synchronization accuracy is limited by quantization error, while int he second region changes in environmental temperature impact the achievable accuracy. We verify our analytic results in simulation and real hardware experiments.
Voluntary Running Prevents Progressive Memory Decline and Increases Adult Hippocampal Neurogenesis and Growth Factor Expression after Whole-Brain Irradiation
Authors: Wong-Goodrich Sarah J.E. , Pfau Madeline L. , Flores Catherine T. , Fraser Jennifer A. , Williams Christina L. , Jones Lee W. 
Published: October 2010

Abstract
Whole-brain irradiation (WBI) therapy produces progressive learning and memory deficits in patients with primary or secondary brain tumors. Exercise enhances memory and adult hippocampal neurogenesis in the intact brain, so we hypothesized that exercise may be an effective treatment to alleviate consequences of WBI. Previous studies using animal models to address this issue have yielded mixed results and have not examined potential molecular mechanisms. We investigated the short- and long-term effects of WBI on spatial learning and memory retention and determined whether voluntary running after WBI aids recovery of brain and cognitive function. Forty adult female C57Bl/6 mice given a single dose of 5 Gy or sham WBI were trained 2.5 weeks and up to 4 months after WBI in a Barnes maze. Half of the mice received daily voluntary wheel access starting 1 month after sham or WBI. Daily running following WBI prevented the marked decline in spatial memory retention observed months after irradiation. Bromodeoxyuridine (BrdUrd) immunolabeling and enzyme-linked immunosorbent assay indicated that this behavioral rescue was accompanied by a partial restoration of newborn BrdUrd+/NeuN+ neurons in the dentate gyrus and increased hippocampal expression of brain-derived vascular endothelial growth factor and insulin-like growth factor-1, and occurred despite irradiation-induced elevations in hippocampal proinflammatory cytokines. WBI in adult mice produced a progressive memory decline consistent with what has been reported in cancer patients receiving WBI therapy. Our findings show that running can abrogate this memory decline and aid recovery of adult hippocampal plasticity, thus highlighting exercise as a potential therapeutic intervention. Cancer Res; 70(22); 9329–38. ©2010 AACR.
Dual Inhibition of PI3K and mTORC1/2 Signaling by NVP-BEZ235 as a New Therapeutic Strategy for Acute Myeloid Leukemia
Authors: Chapuis Nicolas , Tamburini Jerome , Green Alexa S. , Vignon Christine , Bardet Valerie , Neyret Aymeric , Pannetier Melanie , Willems Lise , Park Sophie , Macone Alexandre , Maira Sauveur-Michel , Ifrah Norbert , Dreyfus François , Herault Olivier , Lacombe Catherine , Mayeux Patrick , Bouscary Didier 
Published: October 2010

Abstract
Purpose: The growth and survival of acute myeloid leukemia (AML) cells are enhanced by the deregulation of signaling pathways such as phosphoinositide 3-kinase (PI3K)/Akt and mammalian target of rapamycin (mTOR). Major efforts have thus been made to develop molecules targeting these activated pathways. The mTOR serine/threonine kinase belongs to two separate complexes: mTORC1 and mTORC2. The mTORC1 pathway is rapamycin sensitive and controls protein translation through the phosphorylation of 4E-BP1 in most models. In AML, however, the translation process is deregulated and rapamycin resistant. Furthermore, the activity of PI3K/Akt and mTOR is closely related, as mTORC2 activates the oncogenic kinase Akt. We therefore tested, in this study, the antileukemic activity of the dual PI3K/mTOR ATP-competitive inhibitor NVP-BEZ235 compound (Novartis).
Experimental Design: The activity of NVP-BEZ235 was tested in primary AML samples (n = 21) and human leukemic cell lines. The different signaling pathways were analyzed by Western blotting. The cap-dependent mRNA translation was studied by 7-methyl-GTP pull-down experiments, polysomal analysis, and [3H]leucine incorporation assays. The antileukemic activity of NVP-BEZ235 was tested by analyzing its effects on leukemic progenitor clonogenicity, blast cell proliferation, and survival.
Results: The NVP-BEZ235 compound was found to inhibit PI3K and mTORC1 signaling and also mTORC2 activity. Furthermore, NVP-BEZ235 fully inhibits the rapamycin-resistant phosphorylation of 4E-BP1, resulting in a marked inhibition of protein translation in AML cells. Hence, NVP-BEZ235 reduces the proliferation rate and induces an important apoptotic response in AML cells without affecting normal CD34+ survival.
Conclusions: Our results clearly show the antileukemic efficiency of the NVP-BEZ235 compound, which therefore represents a promising option for future AML therapies. Clin Cancer Res; 16(22); 5424–35. ©2010 AACR.
Mechanisms by which cleaved kininogen inhibits endothelial cell differentiation and signalling
Authors: Wu Yi , Liu Yuchuan , Colman Robert 
Published: October 2010

SummaryWe have shown that cleaved high-molecular-weight kininogen inhibits endoththelial cell tube and vacuole formation in a concentration-dependent manner and this correlates with its recognised anti-angiogenic activity. The antibody against the urokinase plasminogen activator receptor (uPAR) mimicked the inhibitory effect of cleaved kininogen (HKa) on apoptosis (HKa: 30% and uPAR antibody: 26%) and tube formation. In tumour angiogenesis, cancer cells release angiogenic stimulators, such as vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF), thus stimulating the transformation of endogenous pro-uPA to uPA. The proteolytic enzyme urokinase plasminogen activator (uPA) then binds to its receptor in a complex with its inhibitor PAI-1, which results in the internalisation of this complex, and activates extracellular signal-regulated kinase (ERK). Recycling of the uPAR regulates the migration of endothelial cells (ECs). ERK activation stimulates migration and proliferation and suppresses apoptosis of ECs. HKa disrupted the uPA-uPAR complex, inhibited ERK activation, and blocked the internalization of uPAR, eventually resulting in cell death and cell motility arrest. Both are critical steps in angiogenesis.
Urokinase-type plasminogen activator receptor is associated with the development of adipose tissue
Authors: Matsuno Hiroyuki , Kawashita Eri , Okada Kiyotaka , Suga Hidetaka , Ueshima Shigeru , Matsuo Osamu , Kanno Yosuke 
Published: October 2010

SummaryUrokinase-type plasminogen activator receptor (uPAR) plays a role in cellular responses which include cellular adhesion, differentiation, proliferation and migration. The aim of this study was to clarify the role of uPAR on the development of adipose tissue. To clarify the role of uPAR on adipogenesis, we examined the effect of uPAR overexpression and uPAR deficiency on the adipocyte differentiation. Adipocyte differentiation was induced by incubation of 3T3-L1 cells with differentiation media containing insulin, dexamethasone, and 1-methyl-3-isobutylxanthin. uPAR overexpression by transfection of uPAR expression vector induced adipocyte differentiation. In addition, we examined the difference in adipocyte differentiation of mesenchymal stem cells from wild-type mice and uPAR knockout (uPAR-/-) mice. The uPAR deficiency attenuated differentiation media-induced adipocyte differentiation. Moreover, we found that the inhibition of phosphatidylinositol 3-kinase (PI3K) pathway attenuated uPAR overexpression-induced adipocyte differentiation, and uPAR overexpression induced the activation of Akt. We also found that an increase of the adipose tissue mass in uPAR-/- mice was less than that observed in wild-type mice. The present results suggest that uPAR plays a pivotal role in the development of adipose tissue through PI3K/Akt pathway.