bluuebunny/crossref_metadata_2025_split huggingface dataset:
Silver triflate catalyzed synthesis of 3-aminoalkylated indoles and evaluation of their antibacterial activities
Authors: Rao Vagicherla Kameshwara , Rao Madharam Sudershan , Jain Navin , Panwar Jitendra , Kumar Anil 
Published: September 2011

Abstract
An efficient, one-pot synthesis was developed for 3-aminoalkylated indoles by three-component coupling reaction of aldehydes, N-methylanilines, and indoles using AgOTf as a catalyst. A series of twenty 3-aminoalkylated indoles was evaluated for their antibacterial activities against both Gram negative and Gram positive bacteria. Compounds 4b and 4r showed good antibacterial activity against both Gram positive and Gram negative strains. However, inversing the property of substituent (from 4r to 4q) resulted in the significant fall in the magnitude of antibacterial activity against Escherichia coli.
First somatic mutation of E2F1 in a critical DNA binding residue discovered in well-differentiated papillary mesothelioma of the peritoneum
Authors: Yu Willie , Chan-On Waraporn , Teo Melissa , Ong Choon Kiat , Cutcutache Ioana , Allen George E , Wong Bernice , Myint Swe Swe , Lim Kiat Hon , Voorhoeve P Mathijs , Rozen Steve , Soo Khee Chee , Tan Patrick , Teh Bin Tean 
Published: September 2011

Abstract
Background
Well differentiated papillary mesothelioma of the peritoneum (WDPMP) is a rare variant of epithelial mesothelioma of low malignancy potential, usually found in women with no history of asbestos exposure. In this study, we perform the first exome sequencing of WDPMP.

Results
WDPMP exome sequencing reveals the first somatic mutation of E2F1, R166H, to be identified in human cancer. The location is in the evolutionarily conserved DNA binding domain and computationally predicted to be mutated in the critical contact point between E2F1 and its DNA target. We show that the R166H mutation abrogates E2F1's DNA binding ability and is associated with reduced activation of E2F1 downstream target genes. Mutant E2F1 proteins are also observed in higher quantities when compared with wild-type E2F1 protein levels and the mutant protein's resistance to degradation was found to be the cause of its accumulation within mutant over-expressing cells. Cells over-expressing wild-type E2F1 show decreased proliferation compared to mutant over-expressing cells, but cell proliferation rates of mutant over-expressing cells were comparable to cells over-expressing the empty vector.

Conclusions
The R166H mutation in E2F1 is shown to have a deleterious effect on its DNA binding ability as well as increasing its stability and subsequent accumulation in R166H mutant cells. Based on the results, two compatible theories can be formed: R166H mutation appears to allow for protein over-expression while minimizing the apoptotic consequence and the R166H mutation may behave similarly to SV40 large T antigen, inhibiting tumor suppressive functions of retinoblastoma protein 1.
Multistage Triaxial Testing to Estimate Effective Stress Relationships for Unsaturated Compacted Soils
Authors: Khosravi Ali , Alsherif Nahed , Lynch Christopher , McCartney John 
Published: September 2011

AbstractThis paper presents an experimental methodology for using multistage, drained triaxial tests on compacted soils under unsaturated conditions to estimate soil-specific relationships between mean effective stress and matric suction. Tests were performed by applying a matric suction to a soil specimen in a triaxial cell using the axis translation technique with back-pressure, then shearing the specimen under drained conditions until reaching stress-path tangency. The specimen was then unloaded, a new suction was applied, and the shearing process was repeated. The points of maximum principal stress difference for the unsaturated specimen were plotted versus mean effective stress, defined using the degree of saturation as the effective stress parameter, and they were found to correspond well with the critical state line defined from triaxial tests on saturated specimens. The suction stress for the compacted soil tested in this study was observed to increase nonlinearly with suction, tending toward a constant value with increasing suction. Although there are potential changes in soil structure in the specimen during loading, unloading, and reloading, the results indicate that the multistage testing method may be useful for estimating soil-specific effective stress parameters for compacted soils in unsaturated conditions. Furthermore, the fact that differences in the soil-water retention curve of soil specimens subjected to different net confining pressures were observed for the soil tested in this study emphasizes the importance of using soil-specific tests to validate predictive relationships between suction stress and matric suction.
Dynamics of Homology Searching During Gene Conversion inSaccharomyces cerevisiaeRevealed by Donor Competition
Authors: Coïc Eric , Martin Joshua , Ryu Taehyun , Tay Sue Yen , Kondev Jané , Haber James E 
Published: September 2011

AbstractOne of the least understood aspects of homologous recombination is the process by which the ends of a double-strand break (DSB) search the entire genome for homologous templates that can be used to repair the break. We took advantage of the natural competition between the alternative donors HML and HMR employed during HO endonuclease-induced switching of the budding yeast MAT locus. The strong mating-type-dependent bias in the choice of the donors is enforced by the recombination enhancer (RE), which lies 17 kb proximal to HML. We investigated factors that improve the use of the disfavored donor. We show that the normal heterochromatic state of the donors does not impair donor usage, as donor choice is not affected by removing this epigenetic silencing. In contrast, increasing the length of homology shared by the disfavored donor increases its use. This result shows that donor choice is not irrevocable and implies that there are several encounters between the DSB ends and even the favored donor before recombination is accomplished. The increase by adding more homology is not linear; these results can be explained by a thermodynamic model that determines the energy cost of using one donor over the other. An important inference from this analysis is that when HML is favored as the donor, RE causes a reduction in its effective genomic distance from MAT from 200 kb to ∼20 kb, which we hypothesize occurs after the DSB is created, by epigenetic chromatin modifications around MAT.
Differentiation of Carbon Dioxide-Sensing Neurons in Caenorhabditis elegans Requires the ETS-5 Transcription Factor
Authors: Guillermin Manon L , Castelletto Michelle L , Hallem Elissa A 
Published: September 2011

Abstract
Many animals sense environmental gases such as carbon dioxide and oxygen using specialized populations of gas-sensing neurons. The proper development and function of these neurons is critical for survival, as the inability to respond to changes in ambient carbon dioxide and oxygen levels can result in reduced neural activity and ultimately death. Despite the importance of gas-sensing neurons for survival, little is known about the developmental programs that underlie their formation. Here we identify the ETS-family transcription factor ETS-5 as critical for the normal differentiation of the carbon dioxide-sensing BAG neurons in Caenorhabditis elegans. Whereas wild-type animals show acute behavioral avoidance of carbon dioxide, ets-5 mutant animals do not respond to carbon dioxide. The ets-5 gene is expressed in BAG neurons and is required for the normal expression of the BAG neuron gene battery. ets-5 may also autoregulate its expression in BAG neurons. ets-5 is not required for BAG neuron formation, indicating that it is specifically involved in BAG neuron differentiation and the maintenance of BAG neuron cell fate. Our results demonstrate a novel role for ETS genes in the development and function of gas-detecting sensory neurons.
Calcaneal Reconstruction for the Late Complication of Calcaneus Fracture
Authors: Young Ki Won , Lee Kyung Tai , Lee Young Koo , Jang Mun Suk , Yoon Jun Hee , Kim Jun Ho 
Published: September 2011

Calcaneal fracture is the most common fracture in the tarsal bones. Treatment is difficult because the patterns of fracture are various and complications occur frequently. The purpose of this study was to evaluate the clinical results of calcaneal reconstruction for chronic complications after calcaneal fracture.
From September 2001 to November 2004, calcaneal reconstruction was performed in 24 patients (25 feet). We reviewed 21 patients (22 feet) who could be followed up >2 years postoperatively. Patients who underwent subtalar arthrodesis and simple bone resection were excluded. Patients who underwent calcaneal sliding osteotomy were included. Nineteen men and 2 women ranged in age from 27 to 54 years (mean, 44.4 years). The mean interval between the first operation and reconstructive operation was 11.2 months (range, 3–31 months). The mean follow-up period after calcaneal reconstruction was 29.8 months (range, 24–38 months). Böhler angle, calcaneal pitch, and talocalcaneal height were checked pre- and postoperatively.
The most common patient report was pain on the inferior aspect of the lateral malleolus (16 patients) and calcaneal tuberosity (3 patients). All the mean values of talocalcaneal height, calcaneal pitch, and Böhler angle improved, which was statistically significant. In the postoperative period, 10 patients were very satisfied, 9 were satisfied, and 3 were not satisfied. Although pain did not completely resolve, all patients were satisfied postoperatively.
Avascular Necrosis of the Femoral Head in Children With Acute Lymphoblastic Leukemia: A 4- to 9-year Follow-up Study
Authors: Madadi Firooz , Shamsian Bibi Shahin , Alavi Samin , Madadi Firoozeh , Eajazi Alireza , Aslani Afshin 
Published: September 2011

Avascular necrosis of the femoral head is usually seen in children aged 1.5 to 10 years, reaching a peak incidence between the ages of 4 and 9. Avascular necrosis of the femoral head is a known complication of corticosteroid therapy in acute lymphoblastic leukemia. There are few reports in the literature regarding the natural history of this condition, and there is no consensus on its management. This study examined the natural history of avascular necrosis of the femoral head in children with leukemia.
From 1993 to 2006, a total of 865 children with acute lymphoblastic leukemia were admitted to the hematology-oncology ward of a children’s hospital. The diagnosis of acute lymphoblastic leukemia was established by bone marrow aspiration. Based on clinical and radiographic findings, avascular necrosis of the femoral head was found in 7 patients; these patients underwent follow-up for 4 to 9 years. Avascular necrosis of the femoral head was clinically symptomatic in all of the children, and they had advanced radiographic collapse of the femoral head. However, the head of the femur was not at risk in any patient based on clinical and radiographic findings. Patients received supportive treatment such as abduction brace and physiotherapy. After 4 to 9 years of follow-up, clinical and radiographic results were satisfactory. Provided that the head of the femur is not at risk, avascular necrosis of the femoral head in children with acute lymphoblastic leukemia may be successfully managed with nonoperative care.
Is routine viral screening useful in patients with recent‐onset polyarthritis of a duration of at least 6 weeks? Results from a nationwide longitudinal prospective cohort study
Authors: Varache Sophie , Narbonne Valérie , Jousse‐Joulin Sandrine , Guennoc Xavier , Dougados Maxime , Daurès Jean Pierre , Devauchelle‐Pensec Valerie , Saraux Alain 
Published: September 2011

AbstractObjectiveTo study the contribution of routine viral screening tests in patients with early rheumatoid arthritis (RA) or a potential for progressing to RA.MethodsEight hundred thirteen patients with swelling of at least 2 joints for at least 6 weeks and a symptom duration of less than 6 months in the ESPOIR cohort were screened for parvovirus B19 (IgG and IgM anti–parvovirus B19 antibodies), hepatitis B virus (HBV; hepatitis B surface antigen), hepatitis C virus (HCV; anti‐HCV antibodies), and human immunodeficiency virus (HIV; anti–HIV‐1 and ‐2 antibodies).ResultsParvovirus B19 testing was performed in 806 patients and showed longstanding immunity in 574 (71.2%) and no antibodies in 223 (27.7%). Among the 9 remaining patients (7 IgG positive/IgM positive, 1 IgG negative/IgM positive, and 1 IgG indeterminate/IgM positive), only 2 (0.25%; 95% confidence interval [95% CI] 0–0.99%) had a positive polymerase chain reaction test for parvovirus B19; these patients (women ages 34 and 40 years) had no extraarticular signs. HIV seroprevalence was 0.12% (n = 1 of 813; 95% CI 0.01–0.8%) and HCV seroprevalence was 0.86% (n = 7 of 808, 95% CI 0.38–1.86%). HCV‐related arthritis was diagnosed in 4 patients (0.5%). HCV‐seropositive patients had significantly higher transaminase levels than the other patients (P = 0.001), with no significant differences for the other laboratory data. HBV seroprevalence was 0.12% (n = 1 of 808; 95% CI 0.01–0.8%); the positive HBV status was known before study inclusion, and the patient had no diagnosis of HBV‐related arthritis. Finally, routine viral testing identified 2 patients with parvovirus B19 infection and 3 with HBV infection (0.6%; 95% CI 0.2–1.5%). Cost was €85.05 per patient (total €68,720).ConclusionRoutine serologic testing did not contribute substantially to the diagnosis in this context.
Long‐term outcomes and costs of an integrated rehabilitation program for chronic knee pain: A pragmatic, cluster randomized, controlled trial
Authors: Hurley M. V. , Walsh N. E. , Mitchell H. , Nicholas J. , Patel A. 
Published: September 2011

AbstractObjectiveChronic joint pain is a major cause of pain and disability. Exercise and self‐management have short‐term benefits, but few studies follow participants for more than 6 months. We investigated the long‐term (up to 30 months) clinical and cost effectiveness of a rehabilitation program combining self‐management and exercise: Enabling Self‐Management and Coping of Arthritic Knee Pain Through Exercise (ESCAPE‐knee pain).MethodsIn this pragmatic, cluster randomized, controlled trial, 418 people with chronic knee pain (recruited from 54 primary care surgeries) were randomized to usual care (pragmatic control) or the ESCAPE‐knee pain program. The primary outcome was physical function (Western Ontario and McMaster Universities Osteoarthritis Index [WOMAC] function), with a clinically meaningful improvement in physical function defined as a ≥15% change from baseline. Secondary outcomes included pain, psychosocial and physiologic variables, costs, and cost effectiveness.ResultsCompared to usual care, ESCAPE‐knee pain participants had large initial improvements in function (mean difference in WOMAC function −5.5; 95% confidence interval [95% CI] −7.8, −3.2). These improvements declined over time, but 30 months after completing the program, ESCAPE‐knee pain participants still had better physical function (difference in WOMAC function −2.8; 95% CI −5.3, −0.2); lower community‐based health care costs (£−47; 95% CI £−94, £−7), medication costs (£−16; 95% CI £−29, £−3), and total health and social care costs (£−1,118; 95% CI £−2,566, £−221); and a high probability (80–100%) of being cost effective.ConclusionClinical and cost benefits of ESCAPE‐knee pain were still evident 30 months after completing the program. ESCAPE‐knee pain is a more effective and efficient model of care that could substantially improve the health, well‐being, and independence of many people, while reducing health care costs.
Relationship between accelerometer‐based measures of physical activity and the Yale Physical Activity Survey in adults with arthritis
Authors: Semanik Pamela , Lee Jungwha , Manheim Larry , DiPietro Loretta , Dunlop Dorothy , Chang Rowland W. 
Published: September 2011

AbstractObjectiveTo evaluate the correlation between the Yale Physical Activity Survey (YPAS) scores and objective accelerometer measures of time spent in light intensity physical activities, moderate to vigorous intensity physical activities, and moderate to vigorous activities in bouts lasting at least 10 minutes.MethodsThis study analyzed baseline data from 171 persons with rheumatoid arthritis (RA) and 139 persons with osteoarthritis (OA) in a randomized clinical trial (Increasing Motivation for Physical Activity in Arthritis Clinical Trial). Persons fulfilling the 1987 American College of Rheumatology criteria for RA and persons with symptomatic radiologic knee OA (Kellgren/Lawrence class ≥2) wore an accelerometer for 7 days, then responded to the YPAS questionnaire and questions regarding demographics (age, sex, and race) and health factors (body mass index, disease status [Health Assessment Questionnaire/Western Ontario and McMaster Universities Osteoarthritis Index], comorbidities, pain, and function). Spearman's correlation coefficients were estimated between each YPAS summary measure and accelerometer measures.ResultsIn the RA participants, the strongest correlation was between the YPAS activity dimensions summary index (Y‐ADSI) and average daily minutes of bouted moderate/vigorous activity (r = 0.51). Additionally, the Y‐ADSI correlated significantly with both objectively measured average daily accelerometer counts (r = 0.45) and average daily minutes of moderate/vigorous activity (r = 0.43). For OA participants, a similar pattern emerged: the Y‐ADSI had significant correlations with average daily minutes of bouted moderate/vigorous activity (r = 0.36), average daily minutes of moderate/vigorous activity (r = 0.31), and average daily counts (r = 0.24).ConclusionFor both the RA and OA groups, the Y‐ADSI had the strongest significant correlations with objectively measured physical activity, which supports Y‐ADSI use as a tool for clinical applications and in rheumatology research.
Impact of educational and patient decision aids on decisional conflict associated with total knee arthroplasty
Authors: de Achaval Sofia , Fraenkel Liana , Volk Robert J. , Cox Vanessa , Suarez‐Almazor Maria E. 
Published: September 2011

AbstractObjectiveTo examine the impact of a videobooklet patient decision aid supplemented by an interactive values clarification exercise on decisional conflict in patients with knee osteoarthritis (OA) considering total knee arthroplasty.MethodsA total of 208 patients participated in the study (mean age 63 years, 68% female, and 66% white). Participants were randomized to 1 of 3 groups: 1) educational booklet on OA management (control), 2) patient decision aid (videobooklet) on OA management, and 3) patient decision aid (videobooklet) + adaptive conjoint analysis (ACA) tool. The ACA tool enables patients to consider competing attributes (i.e., specific risks/benefits) by asking them to rate a series of paired comparisons. The primary outcome was the decisional conflict scale ranging from 0–100. Differences between groups were analyzed using analysis of variance and Tukey's honestly significant difference tests.ResultsOverall, decisional conflict decreased significantly in all groups (P < 0.05). The largest reduction in decisional conflict was observed for participants in the videobooklet decision aid group (21 points). Statistically significant differences in pre‐ versus postintervention total scores favored the videobooklet group compared to the control group (21 versus 10) and to the videobooklet plus ACA group (21 versus 14; P < 0.001). Changes in the decisional conflict score for the control group compared to the videobooklet decision aid + ACA group were not significantly different.ConclusionIn our study, an audiovisual patient decision aid decreased decisional conflict more than printed material alone or the addition of a more complex computer‐based ACA tool requiring more intense cognitive involvement and explicit value choices.
Joint‐pain comorbidity, health status, and medication use in hip and knee osteoarthritis: A cross‐sectional study
Authors: Hoogeboom Thomas J. , den Broeder Alfons A. , Swierstra Bart A. , de Bie Rob A. , van den Ende Cornelia H. M. 
Published: September 2011

AbstractObjectiveTo determine the prevalence of joint‐pain comorbidities in individuals with hip or knee osteoarthritis (OA) and to assess the differences in the characteristics of people with and without joint‐pain comorbidities.MethodsIn this cross‐sectional study, individuals referred to secondary care for treatment of hip/knee OA completed questionnaires to determine sociodemographic characteristics, disease‐related outcomes, and joint‐pain comorbidities. Joint‐pain comorbidity was defined as pain perceived in a joint, other than the index joint, for more than half of the days in the preceding month. To compare differences in patient‐ and disease‐related characteristics between participants with and without joint‐pain comorbidities, we performed analyses of covariance and logistic regression.ResultsA total of 401 individuals, 117 with hip OA and 284 with knee OA, returned the questionnaire (82% response rate); the mean ± SD age was 58 ± 13 years and 58% of the responders were women. Fifty‐eight percent of the participants reported symptoms in ≥1 other joint. Participants with joint‐pain comorbidities were more likely to be women, less educated, and have more medical comorbidities. Individuals with joint‐pain comorbidities reported unfavorable outcomes on pain, functioning, fatigue, distress, and health‐related quality of life compared with patients without joint‐pain comorbidities (P < 0.001 for all). Moreover, use of nonsteroidal antiinflammatory drugs (P = 0.038), opioids (P = 0.010), and supplements (P = 0.019) was higher in the group with joint‐pain comorbidities.ConclusionOur results indicate that individuals with joint‐pain comorbidities represent a clinically relevant and large subgroup of people with OA of the knee or hip. We recommend addressing joint‐pain comorbidities in both research and clinical practice.