bluuebunny/crossref_metadata_2025_split huggingface dataset:
Patients’ experiences of and roles in interprofessional collaborative practice in primary care: a constructivist grounded theory study
Authors: Davidson Alexandra R. , Morgan Mark , Ball Lauren , Reidlinger Dianne P. 
Published: May 2024

Abstract

Aim:
This constructivist grounded theory study aimed to (1) explore patients’ experiences of and roles in interprofessional collaborative practice for chronic conditions in primary care and (2) consider the relevance and alignment of an existing theoretical framework on patients’ roles and based on the experiences of patient advocates.

Background:
High-quality management of chronic conditions requires an interprofessional collaborative practice model of care considering an individual’s mental, physical, and social health situation. Patients’ experiences of this model in the primary care setting are relatively unknown.

Methods:
A constructivist grounded theory approach was taken. Interview data were collected from primary care patients with chronic conditions across Australia in August 2020 – February 2022. Interviews were recorded, transcribed verbatim, and thematically analysed by (1) initial line-by-line coding, (2) focused coding, (3) memo writing, (4) categorisation, and (5) theme and sub-theme development. Themes and sub-themes were mapped against an existing theoretical framework to expand and confirm the results from a previous study with a similar research aim.

Findings:
Twenty adults with chronic conditions spanning physical disability, diabetes, heart disease, cancer, autoimmune, and mental health conditions participated. Two themes were developed: (1) Adapting to Change with two sub-themes describing how patients adapt to interprofessional team care and (2) Shifting across the spectrum of roles, with five sub-themes outlining the roles patients enact while receiving care. The findings suggest that patients’ roles are highly variable and fluid in interprofessional collaborative practice, and further work is recommended to develop a resource to support greater patient engagement in interprofessional collaborative practice.
Mother, child and adolescent health outcomes in two long-term refugee camp settings at the Thai-Myanmar border 2000–2018: a retrospective analysis
Authors: Benner Marie T. , Mohr Oliver , Kaloy Wiphan , Sansoenboon Ammarat , Moungsookjarean Aree , Kaiser Peter , Carrara Verena I. , McGready Rose 
Published: May 2024

Abstract

Aim:
The study assessed mothers, children and adolescents’ health (MCAH) outcomes in the context of a Primary Health Care (PHC) project and associated costs in two protracted long-term refugee camps, along the Thai-Myanmar border.

Background:
Myanmar refugees settled in Thailand nearly 40 years ago, in a string of camps along the border, where they fully depend on external support for health and social services. Between 2000 and 2018, a single international NGO has been implementing an integrated PHC project.

Methods:
This retrospective study looked at the trends of MCAH indicators of mortality and morbidity and compared them to the sustainable development goals (SDGs) indicators. A review of programme documents explored and triangulated the evolution and changing context of the PHC services, and associated project costs were analysed. To verify changes over time, interviews with 12 key informants were conducted.

Findings:
While maternal mortality (SDG3.1) remained high at 126.5/100,000 live births, child mortality (SDG 3.2) and infectious diseases in children under 5 (SDG 3.3) fell by 69% and by up to 92%, respectively. Maternal anaemia decreased by 30%; and more than 90% of pregnant women attended four or more antenatal care visits, whereas 80% delivered by a skilled birth attendant; caesarean section rates rose but remained low at an average of 3.7%; the adolescent (15–19 years) birth rate peaked at 188 per 1000 in 2015 but declined to 89/1000 in 2018 (SDG 3.7).

Conclusion:
Comprehensive PHC delivery, with improved health provider competence in MCAH care, together with secured funding is an appropriate strategy to bring MCAH indicators to acceptable levels. However, inequities due to confinement in camps, fragmentation of specific health services, prevent fulfilment of the 2030 SDG Agenda to ‘Leave no one behind’. Costs per birth was 115 EURO in 2018; however, MCAH expenditure requires further exploration over a longer period.
Assessment of Nocturnal NOx Heterogeneous Reaction Mechanisms in the Community Multiscale Air Quality (CMAQ) Model
Authors: Hoffman Alicia , Bertram Timothy H. , Holloway Tracey , Harkey Monica 
Published: May 2024

AbstractNitrogen oxides (NOx) have adverse human health impacts and play a central role in the production of ozone and PM2.5. Nighttime heterogeneous chemistry regulates the nocturnal reservoirs and sinks of NOx such as N2O5 removal and ClNO2 production. However, existing parameterizations of nocturnal NOx heterogeneous chemistry in air quality models do not capture the variability in observations. Here, we implemented for the first time in the Community Multiscale Air Quality (CMAQ) model the Gaston N2O5 uptake (γ(N2O5)) mechanism that accounts for the role of particulate organic matter in regulating N2O5 uptake and the Staudt ClNO2 yield (Φ(ClNO2)) mechanism that includes the role of reactive solutes in suppressing ClNO2 production. With the Gaston and Staudt parameterizations, the coarse mode contributed modestly to N2O5 loss (17.2%) but significantly to ClNO2 production (60.3%), highlighting the impact of coarse mode chemistry. The Gaston γ(N2O5) parameterization in the fine mode increased agreement between modeled N2O5 concentration and observations (RMSEnew = 0.37ppb) compared to the model default (RMSEdefault = 0.43ppb). The Gaston γ(N2O5) parameterization was overall biased low due to underestimates in modeled particle oxygen to carbon ratio (O:C). The Staudt Φ(ClNO2) parameterization resulted in further underestimation (NMBnew = −73.7%) compared to the model default (NMBdefault = −37.9%) because of underestimation of fine mode particle chloride concentration. We expect that the updated parameterizations may more accurately capture the mean state and variability in γ(N2O5) and Φ(ClNO2) under conditions where model particulate O:C and chloride are better represented.
Global Ocean Cooling of 2.3°C During the Last Glacial Maximum
Authors: Seltzer A. M. , Davidson P. W. , Shackleton S. A. , Nicholson D. P. , Khatiwala S. 
Published: May 2024

AbstractQuantitative constraints on past mean ocean temperature (MOT) critically inform our historical understanding of Earth's energy balance. A recently developed MOT proxy based on paleoatmospheric Xe, Kr, and N2 ratios in ice core air bubbles is a promising tool rooted in the temperature dependences of gas solubilities. However, these inert gases are systematically undersaturated in the modern ocean interior, and it remains unclear how air‐sea disequilibrium may have changed in the past. Here, we carry out 30 tracer‐enabled model simulations under varying circulation, sea ice cover, and wind stress regimes to evaluate air‐sea disequilibrium in the Last Glacial Maximum (LGM) ocean. We find that undersaturation of all three gases was likely reduced, primarily due to strengthened high‐latitude winds, biasing reconstructed MOT by −0.38 ± 0.37°C (1σ). Accounting for air‐sea disequilibrium, paleoatmospheric inert gases indicate that LGM MOT was 2.27 ± 0.46°C (1σ) colder than the pre‐industrial era.
Decreased apoptotic priming and loss of BCL-2 dependence are functional hallmarks of Richter’s syndrome
Authors: Rigo Antonella , Vaisitti Tiziana , Laudanna Carlo , Terrabuio Eleonora , Micillo Matilde , Frusteri Cristina , D’Ulivo Beatrice , Merigo Flavia , Sbarbati Andrea , Mellert Kevin , Möeller Peter , Montresor Alessio , Di Napoli Arianna , Cirombella Roberto , Butturini Elena , Massaia Massimo , Constantin Gabriela , Vinante Fabrizio , Deaglio Silvia , Ferrarini Isacco 
Published: May 2024

AbstractRichter’s syndrome (RS) is the transformation of chronic lymphocytic leukemia (CLL) into a high-grade B-cell malignancy. Molecular and functional studies have pointed out that CLL cells are close to the apoptotic threshold and dependent on BCL-2 for survival. However, it remains undefined how evasion from apoptosis evolves during disease transformation. Here, we employed functional and static approaches to compare the regulation of mitochondrial apoptosis in CLL and RS. BH3 profiling of 17 CLL and 9 RS samples demonstrated that RS cells had reduced apoptotic priming and lower BCL-2 dependence than CLL cells. While a subset of RS was dependent on alternative anti-apoptotic proteins and was sensitive to specific BH3 mimetics, other RS cases harbored no specific anti-apoptotic addiction. Transcriptomics of paired CLL/RS samples revealed downregulation of pro-apoptotic sensitizers during disease transformation. Albeit expressed, effector and activator members were less likely to colocalize with mitochondria in RS compared to CLL. Electron microscopy highlighted reduced cristae width in RS mitochondria, a condition further promoting apoptosis resistance. Collectively, our data suggest that RS cells evolve multiple mechanisms that lower the apoptotic priming and shift the anti-apoptotic dependencies away from BCL-2, making direct targeting of mitochondrial apoptosis more challenging after disease transformation.
SOAT1 regulates cholesterol metabolism to induce EMT in hepatocellular carcinoma
Authors: Fu Rongrong , Xue Wenqing , Liang Jingjie , Li Xinran , Zheng Juan , Wang Lechen , Zhang Min , Meng Jing 
Published: May 2024

AbstractCholesterol metabolism reprogramming is one of the significant characteristics of hepatocellular carcinoma (HCC). Cholesterol increases the risk of epithelial–mesenchymal transition (EMT) in cancer. Sterol O-acyltransferases 1 (SOAT1) maintains the cholesterol homeostasis. However, the exact mechanistic contribution of SOAT1 to EMT in HCC remains unclear. Here we demonstrated that SOAT1 positively related to poor prognosis of HCC, EMT markers and promoted cell migration and invasion in vitro, which was mediated by the increased cholesterol in plasmalemma and cholesterol esters accumulation. Furthermore, we reported that SOAT1 disrupted cholesterol metabolism homeostasis to accelerate tumorigenesis and development in HCC xenograft and NAFLD-HCC. Also, we detected that nootkatone, a sesquiterpene ketone, inhibited EMT by targeting SOAT1 in vitro and in vivo. Collectively, our finding indicated that SOAT1 promotes EMT and contributes to hepatocarcinogenesis by increasing cholesterol esterification, which is suppressed efficiently by nootkatone. This study demonstrated that SOAT1 is a potential biomarker and therapeutic target in NAFLD-HCC and SOAT1-targeting inhibitors are expected to be the potential new therapeutic treatment for HCC.
Phase-separated droplets swim to their dissolution
Authors: Jambon-Puillet Etienne , Testa Andrea , Lorenz Charlotta , Style Robert W. , Rebane Aleksander A. , Dufresne Eric R. 
Published: May 2024

AbstractBiological macromolecules can condense into liquid domains. In cells, these condensates form membraneless organelles that can organize chemical reactions. However, little is known about the physical consequences of chemical activity in and around condensates. Working with model bovine serum albumin (BSA) condensates, we show that droplets swim along chemical gradients. Active BSA droplets loaded with urease swim toward each other. Passive BSA droplets show diverse responses to externally applied gradients of the enzyme’s substrate and products. In all these cases, droplets swim toward solvent conditions that favor their dissolution. We call this behavior “dialytaxis”, and expect it to be generic, as conditions which favor dissolution typically reduce interfacial tension, whose gradients are well-known to drive droplet motion through the Marangoni effect. These results could potentially suggest alternative physical mechanisms for active transport in living cells, and may enable the design of fluid micro-robots.
Pervasive structural heterogeneity rewires glioblastoma chromosomes to sustain patient-specific transcriptional programs
Authors: Xie Ting , Danieli-Mackay Adi , Buccarelli Mariachiara , Barbieri Mariano , Papadionysiou Ioanna , D’Alessandris Q. Giorgio , Robens Claudia , Übelmesser Nadine , Vinchure Omkar Suhas , Lauretti Liverana , Fotia Giorgio , Schwarz Roland F. , Wang Xiaotao , Ricci-Vitiani Lucia , Gopalakrishnan Jay , Pallini Roberto , Papantonis Argyris 
Published: May 2024

AbstractGlioblastoma multiforme (GBM) encompasses brain malignancies marked by phenotypic and transcriptional heterogeneity thought to render these tumors aggressive, resistant to therapy, and inevitably recurrent. However, little is known about how the spatial organization of GBM genomes underlies this heterogeneity and its effects. Here, we compile a cohort of 28 patient-derived glioblastoma stem cell-like lines (GSCs) known to reflect the properties of their tumor-of-origin; six of these were primary-relapse tumor pairs from the same patient. We generate and analyze 5 kbp-resolution chromosome conformation capture (Hi-C) data from all GSCs to systematically map thousands of standalone and complex structural variants (SVs) and the multitude of neoloops arising as a result. By combining Hi-C, histone modification, and gene expression data with chromatin folding simulations, we explain how the pervasive, uneven, and idiosyncratic occurrence of neoloops sustains tumor-specific transcriptional programs via the formation of new enhancer-promoter contacts. We also show how even moderately recurrent neoloops can relate to patient-specific vulnerabilities. Together, our data provide a resource for dissecting GBM biology and heterogeneity, as well as for informing therapeutic approaches.
CLK2 mediates IκBα-independent early termination of NF-κB activation by inducing cytoplasmic redistribution and degradation
Authors: Li Shang-Ze , Shu Qi-Peng , Zhou Hai-Meng , Liu Yu-Ying , Fan Meng-Qi , Liang Xin-Yi , Qi Lin-Zhi , He Ya-Nan , Liu Xue-Yi , Du Xue-Hua , Huang Xi-Chen , Chen Yu-Zhen , Du Run-Lei , Liang Yue-Xiu , Zhang Xiao-Dong 
Published: May 2024

AbstractActivation of the NF-κB pathway is strictly regulated to prevent excessive inflammatory and immune responses. In a well-known negative feedback model, IκBα-dependent NF-κB termination is a delayed response pattern in the later stage of activation, and the mechanisms mediating the rapid termination of active NF-κB remain unclear. Here, we showed IκBα-independent rapid termination of nuclear NF-κB mediated by CLK2, which negatively regulated active NF-κB by phosphorylating the RelA/p65 subunit of NF-κB at Ser180 in the nucleus to limit its transcriptional activation through degradation and nuclear export. Depletion of CLK2 increased the production of inflammatory cytokines, reduced viral replication and increased the survival of the mice. Mechanistically, CLK2 phosphorylated RelA/p65 at Ser180 in the nucleus, leading to ubiquitin‒proteasome-mediated degradation and cytoplasmic redistribution. Importantly, a CLK2 inhibitor promoted cytokine production, reduced viral replication, and accelerated murine psoriasis. This study revealed an IκBα-independent mechanism of early-stage termination of NF-κB in which phosphorylated Ser180 RelA/p65 turned off posttranslational modifications associated with transcriptional activation, ultimately resulting in the degradation and nuclear export of RelA/p65 to inhibit excessive inflammatory activation. Our findings showed that the phosphorylation of RelA/p65 at Ser180 in the nucleus inhibits early-stage NF-κB activation, thereby mediating the negative regulation of NF-κB.
Shared EEG correlates between non-REM parasomnia experiences and dreams
Authors: Cataldi Jacinthe , Stephan Aurélie M. , Haba-Rubio José , Siclari Francesca 
Published: May 2024

AbstractSleepwalking and related parasomnias result from incomplete awakenings out of non-rapid eye movement sleep. Behavioral episodes can occur without consciousness or recollection, or in relation to dream-like experiences. To understand what accounts for these differences in consciousness and recall, here we recorded parasomnia episodes with high-density electroencephalography (EEG) and interviewed participants immediately afterward about their experiences. Compared to reports of no experience (19%), reports of conscious experience (56%) were preceded by high-amplitude EEG slow waves in anterior cortical regions and activation of posterior cortical regions, similar to previously described EEG correlates of dreaming. Recall of the content of the experience (56%), compared to no recall (25%), was associated with higher EEG activation in the right medial temporal region before movement onset. Our work suggests that the EEG correlates of parasomnia experiences are similar to those reported for dreams and may thus reflect core physiological processes involved in sleep consciousness.
Fourier Quantum Process Tomography
Authors: Di Colandrea Francesco , Dehghan Nazanin , D’Errico Alessio , Karimi Ebrahim 
Published: May 2024

AbstractThe characterization of a quantum device is a crucial step in the development of quantum experiments. This is accomplished via Quantum Process Tomography, which combines the outcomes of different projective measurements to deliver a possible reconstruction of the underlying process. The tomography is typically performed by processing an overcomplete set of measurements and extracting the process matrix from maximum-likelihood estimation. Here, we introduce Fourier Quantum Process Tomography, a technique which requires a reduced number of measurements, and benchmark its performance against the standard maximum-likelihood approach. Fourier Quantum Process Tomography is based on measuring probability distributions in two conjugate spaces for different state preparations and projections. Exploiting the concept of phase retrieval, our scheme achieves a complete and robust characterization of the setup by processing a near-minimal set of measurements. We experimentally test the technique on different space-dependent polarization transformations, reporting average fidelities higher than 90% and significant computational advantage.
Alzheimer’s disease rewires gene coexpression networks coupling different brain regions
Authors: Mitra Sanga , BP Kailash , C R Srivatsan , Saikumar Naga Venkata , Philip Philge , Narayanan Manikandan 
Published: May 2024

AbstractConnectome studies have shown how Alzheimer’s disease (AD) disrupts functional and structural connectivity among brain regions. But the molecular basis of such disruptions is less studied, with most genomic/transcriptomic studies performing within-brain-region analyses. To inspect how AD rewires the correlation structure among genes in different brain regions, we performed an Inter-brain-region Differential Correlation (Inter-DC) analysis of RNA-seq data from Mount Sinai Brain Bank on four brain regions (frontal pole, superior temporal gyrus, parahippocampal gyrus and inferior frontal gyrus, comprising 264 AD and 372 control human post-mortem samples). An Inter-DC network was assembled from all pairs of genes across two brain regions that gained (or lost) correlation strength in the AD group relative to controls at FDR 1%. The differentially correlated (DC) genes in this network complemented known differentially expressed genes in AD, and likely reflects cell-intrinsic changes since we adjusted for cell compositional effects. Each brain region used a distinctive set of DC genes when coupling with other regions, with parahippocampal gyrus showing the most rewiring, consistent with its known vulnerability to AD. The Inter-DC network revealed master dysregulation hubs in AD (at genes ZKSCAN1, SLC5A3, RCC1, IL17RB, PLK4, etc.), inter-region gene modules enriched for known AD pathways (synaptic signaling, endocytosis, etc.), and candidate signaling molecules that could mediate region-region communication. The Inter-DC network generated in this study is a valuable resource of gene pairs, pathways and signaling molecules whose inter-brain-region functional coupling is disrupted in AD, thereby offering a new perspective of AD etiology.
The metal-poor atmosphere of a potential sub-Neptune progenitor
Authors: Barat Saugata , Désert Jean-Michel , Vazan Allona , Baeyens Robin , Line Michael R. , Fortney Jonathan J. , David Trevor J. , Livingston John H. , Jacobs Bob , Panwar Vatsal , Shivkumar Hinna , Todorov Kamen O. , Pino Lorenzo , Mraz Georgia , Petigura Erik A. 
Published: May 2024

AbstractYoung transiting exoplanets offer a unique opportunity to characterize the atmospheres of freshly formed and evolving planets. We present the transmission spectrum of V1298 Tau b, a 23-Myr-old warm Jupiter-sized (0.91 ± 0.05 RJ, where RJ is the radius of Jupiter) planet orbiting a pre-main-sequence star. We detect a mostly clear primordial atmosphere with an exceptionally large atmospheric scale height, and a water vapour absorption at a 5σ level of significance, from which we estimate a planetary mass upper limit (23 Earth masses, 0.12 g cm−3 at a 3σ level). This is one of the lowest-density planets discovered so far. We retrieve a low atmospheric metallicity ($$\log{Z}=-0.{7}_{-0.7}^{+0.8}\,{\mathrm{solar}}$$

log
Z
=
−
0
.

7

−
0.7

+
0.8

solar

), consistent with solar/sub-solar values. Our findings challenge the expected mass–metallicity relation from core-accretion theory. Our observations can instead be explained by in situ formation via pebble accretion together with ongoing evolutionary mechanisms. We do not detect methane, which hints at a hotter-than-expected interior from just the formation entropy of this planet. Our observations suggest that V1298 Tau b is likely to evolve into a sub-Neptune.
Computational design of non-porous pH-responsive antibody nanoparticles
Authors: Yang Erin C. , Divine Robby , Miranda Marcos C. , Borst Andrew J. , Sheffler Will , Zhang Jason Z. , Decarreau Justin , Saragovi Amijai , Abedi Mohamad , Goldbach Nicolas , Ahlrichs Maggie , Dobbins Craig , Hand Alexis , Cheng Suna , Lamb Mila , Levine Paul M. , Chan Sidney , Skotheim Rebecca , Fallas Jorge , Ueda George , Lubner Joshua , Somiya Masaharu , Khmelinskaia Alena , King Neil P. , Baker David 
Published: May 2024

AbstractProgramming protein nanomaterials to respond to changes in environmental conditions is a current challenge for protein design and is important for targeted delivery of biologics. Here we describe the design of octahedral non-porous nanoparticles with a targeting antibody on the two-fold symmetry axis, a designed trimer programmed to disassemble below a tunable pH transition point on the three-fold axis, and a designed tetramer on the four-fold symmetry axis. Designed non-covalent interfaces guide cooperative nanoparticle assembly from independently purified components, and a cryo-EM density map closely matches the computational design model. The designed nanoparticles can package protein and nucleic acid payloads, are endocytosed following antibody-mediated targeting of cell surface receptors, and undergo tunable pH-dependent disassembly at pH values ranging between 5.9 and 6.7. The ability to incorporate almost any antibody into a non-porous pH-dependent nanoparticle opens up new routes to antibody-directed targeted delivery.