bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bats Worldwide Carry Hepatitis E Virus-Related Viruses That Form a Putative Novel Genus within the Family Hepeviridae
Authors: Drexler Jan Felix , Seelen Annika , Corman Victor Max , Fumie Tateno Adriana , Cottontail Veronika , Melim Zerbinati Rodrigo , Gloza-Rausch Florian , Klose Stefan M. , Adu-Sarkodie Yaw , Oppong Samuel K. , Kalko Elisabeth K. V. , Osterman Andreas , Rasche Andrea , Adam Alexander , Müller Marcel A. , Ulrich Rainer G. , Leroy Eric M. , Lukashev Alexander N. , Drosten Christian 
Published: June 2012

ABSTRACTHepatitis E virus (HEV) is one of the most common causes of acute hepatitis in tropical and temperate climates. Tropical genotypes 1 and 2 are associated with food-borne and waterborne transmission. Zoonotic reservoirs (mainly pigs, wild boar, and deer) are considered for genotypes 3 and 4, which exist in temperate climates. In view of the association of several zoonotic viruses with bats, we analyzed 3,869 bat specimens from 85 different species and from five continents for hepevirus RNA. HEVs were detected in African, Central American, and European bats, forming a novel phylogenetic clade in the familyHepeviridae. Bat hepeviruses were highly diversified and comparable to human HEV in sequence variation. No evidence for the transmission of bat hepeviruses to humans was found in over 90,000 human blood donations and individual patient sera. Full-genome analysis of one representative virus confirmed formal classification within the familyHepeviridae. Sequence- and distance-based taxonomic evaluations suggested that bat hepeviruses constitute a distinct genus within the familyHepeviridaeand that at least three other genera comprising human, rodent, and avian hepeviruses can be designated. This may imply that hepeviruses invaded mammalian hosts nonrecently and underwent speciation according to their host restrictions. Human HEV-related viruses in farmed and peridomestic animals might represent secondary acquisitions of human viruses, rather than animal precursors causally involved in the evolution of human HEV.
Andes Virus Infection of Lymphatic Endothelial Cells Causes Giant Cell and Enhanced Permeability Responses That Are Rapamycin and Vascular Endothelial Growth Factor C Sensitive
Authors: Gavrilovskaya Irina N. , Gorbunova Elena E. , Mackow Erich R. 
Published: June 2012

ABSTRACT

Hantaviruses primarily infect endothelial cells (ECs) and nonlytically cause vascular changes that result in hemorrhagic fever with renal syndrome (HFRS) and hantavirus pulmonary syndrome (HPS). Acute pulmonary edema during HPS may be caused by capillary leakage and failure of lymphatic vessels to clear fluids. Uniquely regulated lymphatic ECs (LECs) control fluid clearance, although roles for lymphatics in hantavirus disease remain undetermined. Here we report that hantaviruses productively infect LECs and that LEC infection by HPS causing Andes virus (ANDV) and HFRS causing Hantaan virus (HTNV) are inhibited by α
v
β
3
integrin antibodies. Although α
v
β
3
integrins regulate permeabilizing responses directed by vascular endothelial growth factor receptor 2 (VEGFR2), we found that only ANDV-infected LECs were hyperpermeabilized by the addition of VEGF-A. However, VEGF-C activation of LEC-specific VEGFR3 receptors blocked ANDV- and VEGF-A-induced LEC permeability. In addition, ∼75% of ANDV-infected LECs became viable mononuclear giant cells, >4 times larger than normal, in response to VEGF-A. Giant cells are associated with constitutive mammalian target of rapamycin (mTOR) activation, and we found that both giant LECs and LEC permeability were sensitive to rapamycin, an mTOR inhibitor, and VEGF-C addition. These findings indicate that ANDV uniquely alters VEGFR2-mTOR signaling responses of LECs, resulting in giant cell and LEC permeability responses. This suggests that ANDV infection alters normal LEC and lymphatic vessel functions which may contribute to edematous fluid accumulation during HPS. Moreover, the ability of VEGF-C and rapamycin to normalize LEC responses suggests a potential therapeutic approach for reducing pulmonary edema and the severity of HPS following ANDV infection.
Sequential Evolution and Escape from Neutralization of Simian Immunodeficiency Virus SIVsmE660 Clones in Rhesus Macaques
Authors: Wu Fan , Ourmanov Ilnour , Kuwata Takeo , Goeken Robert , Brown Charles R. , Buckler-White Alicia , Iyengar Ranjini , Plishka Ronald , Aoki Scott T. , Hirsch Vanessa M. 
Published: June 2012

ABSTRACT

Simian immunodeficiency virus (SIV) infection of rhesus macaques has become an important surrogate model for evaluating HIV vaccine strategies. The extreme resistance to neutralizing antibody (NAb) of many commonly used strains, such as SIVmac251/239 and SIVsmE543-3, limits their potential relevance for evaluating the role of NAb in vaccine protection. In contrast, SIVsmE660 is an uncloned virus that appears to be more sensitive to neutralizing antibody. To evaluate the role of NAb in this model, we generated full-length neutralization-sensitive molecular clones of SIVsmE660 and evaluated two of these by intravenous inoculation of rhesus macaques. All animals became infected and maintained persistent viremia that was accompanied by a decline in memory CD4
+
T cells in blood and bronchoalveolar lavage fluid. High titers of autologous NAb developed by 4 weeks postinoculation but were not associated with control of viremia, and neutralization escape variants were detected concurrently with the generation of NAb. Neutralization escape was associated with substitutions and insertion/deletion polymorphisms in the V1 and V4 domains of envelope. Analysis of representative variants revealed that escape variants also induced NAbs within a few weeks of their appearance in plasma, in a pattern that is reminiscent of the escape of human immunodeficiency virus type 1 (HIV-1) isolates in humans. Although early variants maintained a neutralization-sensitive phenotype, viruses obtained later in infection were significantly less sensitive to neutralization than the parental viruses. These results indicate that NAbs exert selective pressure that drives the evolution of the SIV envelope and that this model will be useful for evaluating the role of NAb in vaccine-mediated protection.
Diversity and Relationships of Cocirculating Modern Human Rotaviruses Revealed Using Large-Scale Comparative Genomics
Authors: McDonald Sarah M. , McKell Allison O. , Rippinger Christine M. , McAllen John K. , Akopov Asmik , Kirkness Ewen F. , Payne Daniel C. , Edwards Kathryn M. , Chappell James D. , Patton John T. 
Published: June 2012

ABSTRACT
Group A rotaviruses (RVs) are 11-segmented, double-stranded RNA viruses and are primary causes of gastroenteritis in young children. Despite their medical relevance, the genetic diversity of modern human RVs is poorly understood, and the impact of vaccine use on circulating strains remains unknown. In this study, we report the complete genome sequence analysis of 58 RVs isolated from children with severe diarrhea and/or vomiting at Vanderbilt University Medical Center (VUMC) in Nashville, TN, during the years spanning community vaccine implementation (2005 to 2009). The RVs analyzed include 36 G1P[8], 18 G3P[8], and 4 G12P[8] Wa-like genogroup 1 strains with VP6-VP1-VP2-VP3-NSP1-NSP2-NSP3-NSP4-NSP5/6 genotype constellations of I1-R1-C1-M1-A1-N1-T1-E1-H1. By constructing phylogenetic trees, we identified 2 to 5 subgenotype alleles for each gene. The results show evidence of intragenogroup gene reassortment among the cocirculating strains. However, several isolates from different seasons maintained identical allele constellations, consistent with the notion that certain RV clades persisted in the community. By comparing the genes of VUMC RVs to those of other archival and contemporary RV strains for which sequences are available, we defined phylogenetic lineages and verified that the diversity of the strains analyzed in this study reflects that seen in other regions of the world. Importantly, the VP4 and VP7 proteins encoded by VUMC RVs and other contemporary strains show amino acid changes in or near neutralization domains, which might reflect antigenic drift of the virus. Thus, this large-scale, comparative genomic study of modern human RVs provides significant insight into how this pathogen evolves during its spread in the community.
WARPED SELF-GRAVITATING U(1) GAUGE COSMIC STRINGS IN 5D
Authors: SLAGTER REINOUD JAN , MASSELINK DERK 
Published: June 2012

We present the "classical" Nielsen–Olesen vortex solution on a warped 5-dimensional spacetime, where we solved the effective 4-dimensional equations from the 5-dimensional equations together with the junction and boundary conditions. 4-dimensional cosmic strings show some serious problems concerning the mechanism of string smoothing related to the string mass per unit length, Gμ ≤ 10-6. Moreover, there is no observational evidence of axially symmetric lensing effect caused by cosmic strings. Also super-massive cosmic strings (Gμ ≳ 1), predicted by superstring theory, possess some problems. They are studied because the universe may have undergone phase transitions at scales much higher than the GUT scale. But Gμ ≳ 1 is far above observational bounds, so one needs an inflationary scenario to smooth them out. Further, it is believed that these super-massive strings never extended to macroscopic size. Brane world models could overcome these problems. Gμ could be warped down to GUT scale, even if its value was at the Planck scale. In our warped cosmic string model, where the string mass per unit length in the bulk can be of order of the Planck scale, we find that the 4-dimensional brane spacetime is exponential warped down. Moreover, asymptotically the induced 4-dimensional spacetime does not show conical behavior. So there is no angle deficit compared to its value in the bulk and the spacetime seems to be unphysical, at least under fairly weak assumptions on the stress–energy tensor and without a positive brane tension. The results are confirmed by numerical solutions of the field equations.
Validation of 6-Minute Walk Distance as a Surrogate End Point in Pulmonary Arterial Hypertension Trials
Authors: Gabler Nicole B. , French Benjamin , Strom Brian L. , Palevsky Harold I. , Taichman Darren B. , Kawut Steven M. , Halpern Scott D. 
Published: June 2012

Background—
Nearly all available treatments for pulmonary arterial hypertension have been approved based on change in 6-minute walk distance (Δ6MWD) as a clinically important end point, but its validity as a surrogate end point has never been shown. We aimed to validate the difference in Δ6MWD against the probability of a clinical event in pulmonary arterial hypertension trials.

Methods and Results—

First, to determine whether Δ6MWD between baseline and 12 weeks mediated the relationship between treatment assignment and development of clinical events, we conducted a pooled analysis of patient-level data from the 10 randomized placebo-controlled trials previously submitted to the US Food and Drug Administration (n=2404 patients). Second, to identify a threshold effect for the Δ6MWD that indicated a statistically significant reduction in clinical events, we conducted a meta-regression among 21 drug/dose-level combinations. Δ6MWD accounted for 22.1% (95% confidence interval, 12.1%– 31.1%) of the treatment effect (
P
<0.001). The meta-analysis showed an average difference in Δ6MWD of 22.4 m (95% confidence interval, 17.4–27.5 m), favoring active treatment over placebo. Active treatment decreased the probability of a clinical event (summary odds ratio, 0.44; 95% confidence interval, 0.33–0.57). The meta-regression revealed a significant threshold effect of 41.8 m.

Conclusions—
Our results suggest that Δ6MWD does not explain a large proportion of the treatment effect, has only modest validity as a surrogate end point for clinical events, and may not be a sufficient surrogate end point. Further research is necessary to determine whether the threshold value of 41.8 m is valid for long-term outcomes or whether it differs among trials using background therapy or lacking placebo controls entirely.
Spatial Superposition Method via Model Coupling for Urban Heat Island Albedo Mitigation Strategies
Authors: Silva Humberto , Golden Jay S. 
Published: June 2012

AbstractA spatial superposition design is presented that couples the fifth-generation Pennsylvania State University–National Center for Atmospheric Research Mesoscale Model (MM5) with the National Center of Excellence (NCE) lumped urban thermal model for application to the city of Phoenix, Arizona. This technique utilizes an approach similar to Reynolds decomposition from turbulence theory. The presented decomposition takes the NCE model prediction from a mitigated strategy as the mean temperature and the difference between the NCE and MM5 predictions without mitigation strategy as the perturbed temperature. The goal of this coupled model is to provide spatial variability when simulating mitigation strategies for the urban heat island effect, as compared with the spatially invariant lumped model. A validation analysis was performed incorporating a maximum 35% change from the baseline albedo value for the urban environment. It is shown that the coupled model differs by up to 0.39°C with comparable average surface temperature predictions from MM5. The coupled model was also used to perform analysis of three different albedo-driven spatial mitigation schemes. This resulted in the identification that having a lesser number of mitigated points on a square urban grid in Phoenix with the same average albedo leads to a greater reduction in average hourly temperature.
The Relationship of Rainfall Variability in Western Equatorial Africa to the Tropical Oceans and Atmospheric Circulation. Part II: The Boreal Autumn
Authors: Dezfuli Amin K. , Nicholson Sharon E. 
Published: June 2012

Abstract
This paper examines the mechanisms controlling the year-to-year variability of rainfall over western equatorial Africa during the rainy season of October–December. Five regions with distinct behavior are analyzed separately. Only two show strong associations with the ocean and atmospheric features in the global tropics. These two regions, in the east (the eastern Zaire basin) and west (Angolan coast) of the study area, respectively, demonstrate strikingly opposite relationships with the anomalies of sea surface temperatures (SSTs), sea level pressure (SLP), and east–west atmospheric circulation. The wet (dry) conditions in the eastern Zaire basin are associated with El Niño(La Niña)–like phases. The inverse pattern is apparent for the Angolan coast. The other three regions, lying between these two poles of variability, represent a transition zone with a weak linear relationship to the circulation features.
The vital impact of the east–west circulation cells on rainfall variability results in a stronger association with zonal wind than with SSTs or SLP. In addition to the zonal shift, changes in intensity of the zonal cells also play a crucial role. Variability in both magnitude and location of the circulation cells appear to be modulated by the remote forcing from the Pacific via an atmospheric bridge. However, the eastern sector is impacted mainly when synchronous changes occur in the Indian Ocean, and the western sector is impacted mainly when synchronous changes occur in the Atlantic Ocean.
A Geostrophic Adjustment Model of Two Buoyant Fluids
Authors: Cenedese Claudia , Lerczak James A. , Bartone Giuseppe 
Published: June 2012

Abstract
A combination of analytical calculations and laboratory experiments has been used to investigate the geostrophic adjustment of two buoyant fluids having different densities in a third denser ambient fluid. The frontal position, the depth profile, and the horizontal and vertical alignments of the two buoyant fluids at the final equilibrium state are determined by the ratio of the baroclinic Rossby radii of deformation Γ1 = λ31/λ21 and Γ2 = λ32/λ21 and the Burger numbers B1 = λ31/L1 and B2 = λ32/L2 of the two buoyant fluids, where is the baroclinic Rossby radius of deformation between fluids i and j. The buoyant fluids 1 and 2 have densities ρ1 and ρ2 (ρ1 &lt; ρ2), respectively; the ambient denser fluid has density ρ3; g′ is the reduced gravity; H and L are the buoyant fluids’ initial depth and width, respectively; and f is the Coriolis parameter. Laboratory rotating experiments confirmed the analytical prediction of the location of the two fronts. After reaching geostrophic equilibrium, the two buoyant currents align mainly horizontally when the extent of the fronts between fluids 1 and 3 and between fluids 2 and 3 is large compared to the extent of the front between fluids 1 and 2: that is, large values of λ31 and λ32 compared to λ21 or equivalently Γ1 ≫ 1 and Γ2 ≫ 1. Alternatively, if the extent of the fronts between the three fluids is similar (i.e., Γ1 ≈ Γ2 ≈ 1), the buoyant currents align mainly vertically. Furthermore, the Burger number of the lightest fluid B1 controls the distance of the inner front from the coast, while B2 controls the offshore extent of the outer front.
Evolution of African Easterly Waves in Potential Vorticity Fields
Authors: Tyner Bryce , Aiyyer Anantha 
Published: June 2012

Abstract
The evolution of African easterly waves (AEWs) leading to tropical cyclones (TCs) in the Atlantic during 2000–08 is examined from isentropic potential vorticity (PV) and Lagrangian streamline perspectives. Tropical cyclone formation is commonly preceded by axisymmetrization of PV, scale contraction of the wave, and formation of a closed circulation within the wave. In these cases, PV associated with the synoptic-scale wave is irreversibly deformed and subsumed within the developing vortex. Less commonly, filamentation of the PV leads to separation and independent propagation of the wave and the TC vortex. In an example presented here, the remnant wave with a closed circulation persisted for several days after separation from the TC. A second TC did not result, consistent with several past studies that show that a midtropospheric closed gyre is not sufficient for TC genesis. Sometimes, an AEW and a weak TC remain coupled for a few days, followed by the dissipation of the TC and the continued propagation of the wave. Merger of tropical and extratropical PV anomalies is also often observed and likely helps maintain some waves. The results of this study are broadly consistent with recent Lagrangian analyses of AEW evolution during TC genesis.
Weather, Climate, and Worldviews: The Sources and Consequences of Public Perceptions of Changes in Local Weather Patterns*
Authors: Goebbert Kevin , Jenkins-Smith Hank C. , Klockow Kim , Nowlin Matthew C. , Silva Carol L. 
Published: June 2012

Abstract
This paper analyzes the changes Americans perceive to be taking place in their local weather and tests a series of hypotheses about why they hold these perceptions. Using data from annual nationwide surveys of the American public taken from 2008 to 2011, coupled with geographically specific measures of temperature and precipitation changes over that same period, the authors evaluate the relationship between perceptions of weather changes and actual changes in local weather. In addition, the survey data include measures of individual-level characteristics (age, education level, gender, and income) as well as cultural worldview and political ideology. Rival hypotheses about the origins of Americans’ perceptions of weather change are tested, and it is found that actual weather changes are less predictive of perceived changes in local temperatures, but better predictors of perceived flooding and droughts. Cultural biases and political ideology also shape perceptions of changes in local weather. Overall, the analysis herein indicates that beliefs about changes in local temperatures have been more heavily politicized than is true for beliefs about local precipitation patterns. Therefore, risk communications linking changes in local patterns of precipitation to broader changes in the climate are more likely to penetrate identity-protective cognitions about climate.
Somatic presentations of distress in China
Authors: Zaroff Charles M , Davis J Mark , Chio Pit Hoi , Madhavan Deepak 
Published: June 2012

Objective: Somatic presentations of distress are common cross-culturally and are thought to predominate in Asian cultures such as that of China. From an etic perspective, researchers utilizing empirically validated standardized assessment measures find that somatic symptoms are no more common in individuals of Chinese descent than they are in individuals of European descent. In contrast, patient presentations are heavily influenced by culture and are associated with patterns of illness behavior. The objective of the current review is to determine the culture-specific factors contributing to somatic presentations and descriptions of distress in China. Method: The current review was based on a literature search of PubMed and PsychInfo using the terms ‘China,’ ‘Asia,’ ‘somatoform,’ ‘somatization,’ and ‘psychogenic.’ Results: Factors contributing to somatic presentations of distress in China include stigma and help-seeking behavior, and assessment approaches that ignore culture-specific patterns of symptom reporting, fail to incorporate somatic metaphor and Chinese conceptualizations of distress that emphasize bodily sensation, and ignore the role that culture-specific normative data and culture specific response patterns may produce on assessment results. Conclusions: From an emic perspective, there are numerous factors contributing to the appearance of a predominantly somatic presentation of distress in China. Implications for clinical practice are discussed.
Assessing the cost of early intervention in psychosis: A systematic review
Authors: Amos Andrew 
Published: June 2012

Objective: Early-intervention units have proliferated over the last decade, justified in terms of cost as well as treatment effect. Strong claims for extension of these programmes on economic grounds motivate a systematic review of economic evaluations of early-intervention programmes. Method: Searches were undertaken in the Cochrane Central Register of Controlled Trials, PubMed, EMBASE, and PsycINFO with keywords including ‘early intervention’, ‘ultra-high risk’, ‘prodrome’, ‘cost-effectiveness’, ‘psychosis’, ‘economic’, and ‘at-risk mental state’. Relevant journals, editorials, and the references of retrieved articles were hand-searched for appropriate research. Results: Eleven articles were included in the review. The more rigorous research (two randomized control trials and two quasi-experimental studies) suggested no difference in resource utilization or costs between early-intervention and treatment-as-usual groups. One small case-control study with evidence of significant bias concluded annual early-intervention costs were one-third of treatment-as-usual costs. Modelling studies projected reduced costs of early intervention but were based on assumptions since definitively revised. Cost-effectiveness analyses did not strongly support the cost-effectiveness of early intervention. No studies appropriately valued outpatient costs or addressed the feasibility of realizing reduced hospitalization in reduced costs. Conclusions: The published literature does not support the contention that early intervention for psychosis reduces costs or achieves cost-effectiveness. Past failed attempts to reduce health costs by reducing hospitalization, and increased outpatient costs in early-intervention programmes suggest such programmes may increase costs. Future economic evaluation of early-intervention programmes would need to correctly value outpatient costs and accommodate uncertainty regarding reduced hospitalization costs, perhaps by sensitivity analysis. The current research hints that cost differences may be greater early in treatment and in patients with more severe illness.