bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development and utility of a multi‐dimensional grid to assess individual mineral metabolism control in hemodialysis patients: A potential aid for therapeutic decision making?
Authors: MORTON A. Ross , MURPHY Sean , HIRSCH David , LEBLANC Martine , BARRÉ Paul , LOK Charmaine , HERCZ Gavril , HODSMAN Anthony , RESLEROVA Martina , LEVIN Adeera 
Published: March 2010

AbstractA grid was developed to evaluate control of serum calcium, phosphate, and parathyroid hormone levels in hemodialysis patients, based on guideline recommendations (National Kidney Foundation Kidney Disease Outcomes Quality Initiative and Canadian Society of Nephrology), and its face validity was examined in a representative sample of Canadian patients. A retrospective chart review was undertaken in hemodialysis patients from 7 Canadian units. Patients >18 years, on hemodialysis for ≥12 months, and ≥3 parathyroid hormone levels measured ≥1 month apart were included. The grid classified mineral metabolism control as optimal, suboptimal, or poor (mean of 3 measurements). Medication use, hospitalization, and Emergency Department visits were evaluated in relation to grid occupancy. A second comparative analysis of grid occupancy was undertaken on prevalent hemodialysis cases in British Columbia in 2008. Data from 268 patients (mean age 62.3 years) were analyzed. Using National Kidney Foundation Kidney Disease Outcomes Quality Initiative guidelines, 17.5%, 28.8%, and 53.7% of patients had optimal, suboptimal, and poor control, respectively, of all 3 parameters (calcium, phosphate, and parathyroid hormone). Using Canadian Society of Nephrology criteria, optimal, suboptimal, and poor control rates were 6.3%, 4.2%, and 89.5%, respectively. Poor control was a possible or a probable cause of hospitalization or Emergency Department attendance in 8 patients. Data from British Columbia in 2008 (n=1858) show optimal, suboptimal, and poor control rates of 15.8%, 24.5%, and 59.7%, respectively. Poor mineral metabolism control among Canadian hemodialysis patients is not showing improvement. The therapeutic grid is a valid tool and may help guide therapeutic decisions, quality control initiatives, and patient counseling. http://www.ukidney.com/bone‐and‐mineral‐metabolism‐resource.
ThaDD plus high dose therapy and autologous stem cell transplantation does not appear superior to ThaDD plus maintenance in elderly patients with de novo multiple myeloma
Authors: Offidani Massimo , Leoni Pietro , Corvatta Laura , Polloni Claudia , Gentili Silvia , Savini Agnese , Alesiani Francesco , Brunori Marino , Catarini Massimo , Visani Giuseppe , Samori Arduino , Burattini Maurizio , Centurioni Riccardo , Montanari Mauro , Fraticelli Paolo , Ruggieri Miriana , Falcioni Sadia , Galieni Piero 
Published: March 2010

Abstract Objectives: With the aim to address the issue whether high‐dose therapy (HDT) is required after new drugs combinations to improve outcome of elderly newly diagnosed multiple myeloma (MM) patients, we compared the toxicity and the outcome of ThaDD plus maintenance to those of ThaDD plus HDT‐autologous stem cell transplantation (ASCT). Methods: Sixty‐two patients not eligible for HDT receiving six courses of ThaDD regimen plus maintenance with thalidomide were compared to 26 patients eligible for HDT treated with four courses of ThaDD followed by melphalan 100–200 mg/m2 and ASCT. The two groups were matched for the main characteristics except for age favouring the HDT group. Results and conclusions: Complete remission (CR) obtained with ThaDD plus maintenance was 24% whereas it was 57% after ThaDD plus HDT‐ASCT (P = 0.0232). However, after a median follow‐up of 36 months, median time to progression (TTP) and progression free survival (PFS) of the group of patients undergone HDT were not significantly different to those of patients receiving ThaDD plus maintenance (32 vs. 31 months: P = 0.962; 32 vs. 29 months: P = 0.726, respectively). Five‐year overall survival (OS) was 49% in the first group and 46% in the latter one (P = 0.404). As expected, a significantly higher incidence of grade 3–4 neutropenia, thrombocytopenia, infections, mucositis and alopecia were observed in the ThaDD plus HDT group. Our results suggest that in elderly MM patients ThaDD plus HDT, albeit significantly increases CR rate, seems to be equivalent to ThaDD plus maintenance in terms of TTP, PFS and OS. These results challenge the requirement for HDT consolidation in this subset of patients.
Chromosome 9p24 abnormalities: prevalence, description of novel JAK2 translocations, JAK2V617F mutation analysis and clinicopathologic correlates
Authors: Patnaik Mrinal M. , Knudson Ryan A. , Gangat Naseema , Hanson Curtis A. , Pardanani Animesh , Tefferi Ayalew , Ketterling Rhett P. 
Published: March 2010

Abstract Objectives:  Current information is insufficient regarding the prevalence, cytogenetic details, JAK2 involvement, JAK2V617F mutational status, and clinicopathologic correlates of chromosome 9p24 abnormalities. The current study was designed to provide additional data in this regard. Methods:  The Mayo Clinic cytogenetics database spanning the years 1989–2008 was screened for chromosome 9p24 abnormalities. Bone marrow (BM) morphology was re‐reviewed and relevant clinical information retrospectively obtained. Structural abnormalities of JAK2 were examined by FISH and JAK2V617F mutation analysis. Results:  Among 24 262 unique patient cytogenetic studies, chromosome 9p24 abnormalities were identified in 25 patients (∼0.06%): 12 with myeloid and 13 with lymphoid neoplasms. The associated karyotype was complex in 12 of the 13 lymphoid but in only four of the 12 myeloid cases. Archived BM was available in 23 cases that allowed additional FISH studies and JAK2V617F mutation analysis. FISH analysis disclosed JAK2 involvement in 10 patients including five myeloid and five lymphoid cases; the associated karyotype was complex in all the lymphoid but in none of the myeloid cases. Five patients displayed previously undescribed JAK2 translocations: t(8;9)(q22;p24), t(9;17)(p24;q23), t(4;9)(q25;p24), t(2;9)(p21;p24) and t(8;9)(q13;p24). JAK2‐associated chromosomal translocations, in a non‐complex karyotype setting, were documented in four patients, all of whom carried a diagnosis of myeloproliferative neoplasms (MPN) and harbored JAK2V617F. Two patients with diffuse large B cell lymphoma displayed complex karyotype and JAK2 amplification by FISH. Conclusions:  Chromosome 9p24 abnormalities are rare and do not always involve JAK2. The subset with JAK2 translocations are usually associated with MPN and harbor JAK2V617F, suggesting a cause‐effect relationship. The current study also describes five novel translocations involving JAK2.
Applying an excessive mechanical stress alters the effect of subchondral osteoblasts on chondrocytes in a co‐culture system
Authors: Lin Yu‐Yu , Tanaka Nobuaki , Ohkuma Satoru , Iwabuchi Yasunori , Tanne Yuki , Kamiya Takashi , Kunimatsu Ryo , Huang Yu‐Ching , Yoshioka Motoko , Mitsuyoshi Tomomi , Tanimoto Kotaro , Tanaka Eiji , Tanne Kazuo 
Published: March 2010

Lin Y‐Y, Tanaka N, Ohkuma S, Iwabuchi Y, Tanne Y, Kamiya T, Kunimatsu R, Huang Y‐C, Yoshioka M, Mitsuyoshi T, Tanimoto K, Tanaka E, Tanne K. Applying an excessive mechanical stress alters the effect of subchondral osteoblasts on chondrocytes in a co‐culture system. Eur J Oral Sci 2010; 118: 151–158. © 2010 The Authors. Journal compilation © 2010 Eur J Oral Sci Osteoarthritis (OA) sometimes occurs as a consequence of repeated microtrauma involved in parafunction, which may lead to microfracture in the subchondral bone. The aim of this in vitro study was to evaluate the effects of subchondral osteoblasts in loading with repeated excessive mechanical stress on the metabolism of overlying chondrocytes. A high‐magnitude cyclic tensile stress of 15 kPa (30 cycles min−1) was applied to the cultured osteoblasts obtained from porcine mandibular condyles. The chondrocytes in alginate beads were then co‐cultured with mechanically stressed or unstressed osteoblasts. Chondrocytes co‐cultured with unstressed osteoblasts showed a phenotypic shift to hypertrophic chondrocytes, characterized by decreased expression of type II collagen, aggrecan, Sry‐related HMG box (SOX‐9), and cartilage oligomeric matrix protein (COMP) genes and increased expression of type X collagen and bone sialoprotein (BSP) genes, suggesting that the co‐culture may change the chondrocyte differentiation to some extent. These changes were more distinct in chondrocytes co‐cultured with excessively mechanically stressed osteoblasts. After co‐culture with stressed osteoblasts, the expressions of matrix metalloproteinase (MMP)1, MMP3 and MMP13 genes were also enhanced and the synthesis of DNA, proteoglycan and collagen were significantly decreased in chondrocytes. These results demonstrate that alterations in cartilage metabolism can be induced by stressed osteoblasts, indicating a possible explanation for the onset and progression of OA.
Enamel microhardness and bond strengths of self‐etching primer adhesives
Authors: Adebayo Olabisi A. , Burrow Michael F. , Tyas Martin J. , Adams Geoffrey G. , Collins Marnie L. 
Published: March 2010

Adebayo OA, Burrow MF, Tyas MJ, Adams GG, Collins ML. Enamel microhardness and bond strengths of self‐etching primer adhesives. Eur J Oral Sci 2010; 118: 191–196. © 2010 The Authors. Journal compilation © 2010 Eur J Oral Sci The aim of this study was to determine the relationship between enamel surface microhardness and microshear bond strength (μSBS). Buccal and lingual mid‐coronal enamel sections were prepared from 22 permanent human molars and divided into two groups, each comprising the buccal and lingual enamel from 11 teeth, to analyze two self‐etching primer adhesives (Clearfil SE Bond and Tokuyama Bond Force). One‐half of each enamel surface was tested using the Vickers hardness test with 10 indentations at 1 N and a 15‐s dwell time. A hybrid resin composite was bonded to the other half of the enamel surface with the adhesive system assigned to the group. After 24 h of water storage of specimens at 37º°C, the μSBS test was carried out on a universal testing machine at a crosshead speed of 1 mm min−1 until bond failure occurred. The mean μSBS was regressed on the mean Vickers hardness number (VHN) using a weighted regression analysis in order to explore the relationship between enamel hardness and μSBS. The weights used were the inverse of the variance of the μSBS means. Neither separate correlation analyses for each adhesive nor combined regression analyses showed a significant correlation between the VHN and the μSBS. These results suggest that the μSBS of the self‐etch adhesive systems are not influenced by enamel surface microhardness.
Down‐regulation of hypoxia inducible factor‐1α: a possible explanation for the protective effects of estrogen on genioglossus fatigue resistance
Authors: Jia Shan‐Shan , Liu Yue‐Hua 
Published: March 2010

Jia S‐S, Liu Y‐H. Down‐regulation of hypoxia inducible factor‐1α: a possible explanation for the protective effects of estrogen on genioglossus fatigue resistance. Eur J Oral Sci 2010; 118: 139–144. © 2010 The Authors. Journal compilation © 2010 Eur J Oral Sci Patients with obstructive sleep apnea/hypopnea syndrome (OSAHS) often exhibit fatigued or inefficient upper airway dilator muscle activity. It has been shown that estrogen may have some impact on upper airway contractility under normoxic conditions. Chronic intermittent hypoxia (CIH) is a frequent feature of OSAHS, and it may alter muscle susceptibility to oxidative stress, a characteristic of a fatigable nature. Hypoxia inducible factor‐1 (HIF‐1) is a transcription factor that is responsible for the regulation of oxygen homeostasis under hypoxic conditions. We examined the effects of estrogen on the contractility of the genioglossus by exposing rats to alternating cycles of 6–8% O2 every 15 s for a total duration of 35 d. The results showed that muscle fatigue resistance was significantly decreased after CIH but was partially reversed after estrogen treatment. Compared with the control group, real‐time reverse transcription–polymerase chain reaction and western blotting showed higher levels of HIF‐1α messenger RNA and protein in the CIH group, but estrogen treatment reduced, in a dose‐independent manner, the levels of HIF‐1α messenger RNA and protein in rats exposed to CIH. We conclude that CIH induced the expression of HIF‐1α in the genioglossus and altered the physical properties towards a more fatigable phenotype, whereas estrogen inhibited the over‐expression of HIF‐1α, and this may account for the improvement of upper airway muscle endurance in CIH rats.
Cholinergic autoantibodies from primary Sjögren’s syndrome modulate submandibular gland Na+/K+‐ATPase activity via prostaglandin E2 and cyclic AMP
Authors: Passafaro Daniela , Reina Silvia , Sterin‐Borda Leonor , Borda Enri 
Published: March 2010

Passafaro D, Reina S, Sterin‐Borda L, Borda E. Cholinergic autoantibodies from primary Sjögren’s syndrome modulate submandibular gland Na

+

/K

+

‐ATPase
activity via prostaglandin E

2

and cyclic AMP. Eur J Oral Sci 2010; 118: 131–138. © 2010 The Authors. Journal compilation © 2010 Eur J Oral Sci We demonstrate that patients with primary Sjögren’s syndrome (pSS) produce functional IgG autoantibodies that interact with the glandular M3 muscarinic acetylcholine receptors (mAChRs). These autoantibodies act as a partial muscarinic agonist, increasing prostaglandin E2 (PGE2) and cyclic AMP production through modifying Na+/K+‐ATPase activity, but also interfere with the secretory effect of the parasympathetic neurotransmitter. The IgG from patients with pSS has two effects on the submandibular gland. On the one hand, it may act as an inducer of the proinflammatory molecule (PGE2) that, in turn, inhibits Na+/K+‐ATPase activity. On the other hand, it plays a role in the pathogenesis of dry mouth, abolishing the Na+/K+‐ATPase inhibition and the net K+ efflux stimulation of the salivary gland in response to the authentic agonist pilocarpine, decreasing salivary fluid production.
Altered inorganic composition of dental enamel and dentin in primary teeth from girls with Turner syndrome
Authors: Rizell Sara , Kjellberg Heidrun , Dietz Wolfram , Norén Jörgen G. , Lundgren Ted 
Published: March 2010

Rizell S, Kjellberg H, Dietz W, Norén JG, Lundgren T. Altered inorganic composition of dental enamel and dentin in primary teeth from girls with Turner syndrome. Eur J Oral Sci 2010; 118: 183–190. © 2010 The Authors. Journal compilation © 2010 Eur J Oral SciIn Turner syndrome (TS) one X‐chromosome is missing or defective. The amelogenin gene, located on the X‐chromosome, plays a key role during the formation of dental enamel. The aim of this study was to find support for the hypothesis that impaired expression of the X‐chromosome influences mineral incorporation during amelogenesis and, indirectly, during dentinogenesis. Primary tooth enamel and dentin from girls with TS were analysed and compared with the enamel and dentin of primary teeth from healthy girls. Qualitative and quantitative changes in the composition of TS enamel were found, in addition to morphological differences. Higher frequencies of subsurface lesions and rod‐free zones were seen in TS enamel using polarized light microscopy. Similarly, scanning electron microscopy showed that the enamel rods from TS teeth were of atypical sizes and directions. Using X‐ray microanalysis, high levels of calcium and phosphorus, and low levels of carbon, were found in both TS enamel and dentin. Using microradiography, a lower degree of mineralization was found in TS enamel. Rule induction analysis was performed to identify characteristic element patterns for TS. Low values of carbon were the most critical attributes for the outcome TS. The conclusion was that impaired expression of the X‐chromosome has an impact on dental hard tissue formation.
Flexural strength of glass fibre‐reinforced posts bonded to dual‐cure composite resin cements
Authors: Davis Peter , Melo Luciana S. D. , Foxton Richard M. , Sherriff Martyn , Pilecki Peter , Mannocci Francesco , Watson Timothy F. 
Published: March 2010

Davis P, Melo LSD, Foxton RM, Sherriff M, Pilecki P, Mannocci F, Watson TF. Flexural strength of glass fibre‐reinforced posts bonded to dual‐cure composite resin cements. Eur J Oral Sci 2010; 118: 197–201. © 2010 The Authors. Journal compilation © 2010 Eur J Oral Sci The aims of this study were to evaluate the flexural strength of two different types of glass fibre‐reinforced posts bonded to dual‐cure composite resin cements. Forty glass methacrylate‐based fibre posts (GC Fiber Post) and 20 glass fibre inter‐polymerizing network posts (everStick POST) were divided into three groups. Group 1 contained 20 GC posts that were bonded to a dual‐cure composite cement (UnifilCore). Group 2 contained 20 Stick Tech posts that had adhesive applied (Scotchbond Multipurpose resin) and were bonded to a dual‐cure composite resin cement (RelyX Unicem). Group 3 contained 20 GC posts that were pretreated with a silane‐coupling agent before being treated with resin and composite, as in group 1. A 4‐point bend test was carried out to failure on all of the groups. Failure modes were determined using scanning electron microscopy. Pretreatment of the post surface with the silane‐coupling agent did not increase the flexural strength. The flexural strength of the Stick Tech post was significantly lower than the flexural strength of the GC post. The mode of failure for the GC Posts was adhesive, whereas the Stick Tech posts failed cohesively. Different flexural strengths and failure modes were observed among the two fibre post–resin systems.
Thermal/mechanical simulation and laboratory fatigue testing of an alternative yttria tetragonal zirconia polycrystal core‐veneer all‐ceramic layered crown design
Authors: Bonfante Estevam A. , Rafferty Brian , Zavanelli Ricardo A. , Silva Nelson R. F. A. , Rekow Elizabeth D. , Thompson Van P. , Coelho Paulo G. 
Published: March 2010

Bonfante EA, Rafferty B, Zavanelli RA, Silva NRFA, Rekow ED, Thompson VP, Coelho PG. Thermal/mechanical simulation and laboratory fatigue testing of an alternative yttria tetragonal zirconia polycrystal
core‐veneer all‐ceramic layered crown design. Eur J Oral Sci 2010; 118: 202–209. © 2010 The Authors. Journal compilation © 2010 Eur J Oral Sci This study evaluated the stress levels at the core layer and the veneer layer of zirconia crowns (comprising an alternative core design vs. a standard core design) under mechanical/thermal simulation, and subjected simulated models to laboratory mouth‐motion fatigue. The dimensions of a mandibular first molar were imported into computer‐aided design (CAD) software and a tooth preparation was modeled. A crown was designed using the space between the original tooth and the prepared tooth. The alternative core presented an additional lingual shoulder that lowered the veneer bulk of the cusps. Finite element analyses evaluated the residual maximum principal stresses fields at the core and veneer of both designs under loading and when cooled from 900°C to 25°C. Crowns were fabricated and mouth‐motion fatigued, generating master Weibull curves and reliability data. Thermal modeling showed low residual stress fields throughout the bulk of the cusps for both groups. Mechanical simulation depicted a shift in stress levels to the core of the alternative design compared with the standard design. Significantly higher reliability was found for the alternative core. Regardless of the alternative configuration, thermal and mechanical computer simulations showed stress in the alternative core design comparable and higher to that of the standard configuration, respectively. Such a mechanical scenario probably led to the higher reliability of the alternative design under fatigue.
REVIEW ARTICLE: The Role of Placental Exosomes in Reproduction
Authors: Mincheva‐Nilsson Lucia , Baranov Vladimir 
Published: March 2010

Citation Mincheva‐Nilsson L, Baranov V. The Role of Placental Exosomes in Reproduction. Am J Reprod Immunol 2010Cell communication comprises cell–cell contact, soluble mediators and intercellular nanotubes. There is, however, another cell–cell communication by released membrane‐bound microvesicles that convey cell–cell contact ‘by proxy’ transporting signals/packages of information from donor to recipient cells locally and/or at a distance. The nanosized exosomes comprise a specialized type of microvesicles generated within multivesicular bodies (MVB) and released upon MVB fusion with the plasma membrane. Exosomes are produced by a variety of immune, epithelial and tumor cells. Upon contact, exosomes transfer molecules that can render new properties and/or reprogram their recipient cells. Recently, it was discovered that the syncytiotrophoblast constitutively and throughout the pregnancy secretes exosomes. The placenta‐derived exosomes are immunosuppressive and carry proteins and RNA molecules that in a redundant way influence a number of mechanisms and promote the fetal allograft survival. In this review, we summarize the current knowledge on the nature of placenta‐derived exosomes and discuss their role in pregnancy.
LINE‐1 methylation difference between ameloblastoma and keratocystic odontogenic tumor
Authors: Kitkumthorn N , Mutirangura A 
Published: March 2010

Oral Diseases (2010) 16, 286–291Objective:  Global hypomethylation is a common epigenetic event in cancer. Keratocystic odontogenic tumor (KCOT) and ameloblastoma are different tumors but posses the same tissue in origin. Here, we investigated long interspersed nuclear element‐1 (LINE‐1 or L1) methylation status between ameloblastoma and KCOT.Materials and methods:  We studied the methylation levels of the long interspersed nucleotide element‐1 (LINE‐1) in ameloblastoma and KCOT. After collecting ameloblastoma cells and epithelium lining cells of KCOT by laser capture microdissection from paraffin embedded tissue, combined bisulfite restriction analysis of LINE‐1 (COBRALINE‐1) was performed to measure LINE‐1 methylation levels.Results:  The LINE‐1 methylation level in KCOT (53.16 ± 12.03%) was higher than that in ameloblastoma (36.90 ± 16.52%), with a statistical significance of P = 0.001. The ranges of LINE‐1 methylation of both lesions were not associated with either age or sex.Conclusion:  We found LINE‐1 hypomethylation levels between ameloblastoma and KCOT are different. Therefore, global methylations between these tumors are processed differently.
Altered expression of mRNA for HIF‐1α and its target genes RTP801and VEGF in patients with oral lichen planus
Authors: Ding M , Xu JY , Fan Y 
Published: March 2010

Oral Diseases (2010) 16, 299–304Objective:  To explore a potential causal contribution of the transcription factor HIF‐1α and its target gene, RTP801 and VEGF, to the development of oral lichen planus (OLP).Design relevant:  Twenty‐two adult OLP patients were enrolled in this study. All OLP diagnoses were verified by histopathological characteristics. Normal mucous specimens were collected from 12 controls after various oral surgeries.Material and method:  RNA was isolated from OLP and control specimens. Microarray was performed using BiostarH‐40s gene chip. Expression of HIF‐1α, VEGF and RTP801 was evaluated using quantitative real‐time polymerase chain reaction (qPCR). Unpaired t‐test and one‐way ANOVA was used for statistical analysis.Results:  Microarray results showed that RTP801 expression was lower in OLP than in controls (779 vs 3090). qPCR further confirmed that expression of RTP801 was similarly lower in OLP than in controls (0.363 vs 1.473, P < 0.001); expression of VEGF was also lower in OLP (0.448 vs 1.74, P = 0.012). In contrast, expression of HIF‐1α was higher in OLP than in controls (11.12 vs 1.628, P < 0.001).Conclusion:  The oral mucosa of OLP is hypoxic. Genes that are activated by hypoxia, such as RTP801 and VEGF, and their signal cascades may be novel potential therapeutic targets for OLP.