bluuebunny/crossref_metadata_2025_split huggingface dataset:
Terbinafine inhibits oral squamous cell carcinoma growth through anti‐cancer cell proliferation and anti‐angiogenesis
Authors: Chien Ming‐Hsien , Lee Tong‐Sheng , Kao Chieh , Yang Shun‐Fa , Lee Wen‐Sen 
Published: May 2011

AbstractTerbinafine (TB), an oral antifungal agent used in the treatment of superficial mycosis, has been reported to exert an anti‐tumor effect in various cancer cells. However, the effect of TB on oral cancer has not been evaluated. Herein we demonstrate that TB (0–60 µM) concentration‐dependently decreased cell number in cultured human oral squamous cell carcinoma (OSCC), KB cells. The anti‐proliferation effect of TB was also observed in two other OSCC cell lines, SAS and SCC 15. TB (60 µM) was not cytotoxic and its inhibition on KB cell growth was reversible. [3H]thymidine incorporation and flow cytometric analyses revealed that TB‐inhibited DNA synthesis and induced the G0/G1 cell‐cycle arrest. The TB‐induced cell‐cycle arrest occurred when the cyclin‐dependent kinase 2 activity was inhibited just as the protein levels of p21cip1 and p27kip1 were increased. The TB‐induced G0/G1 cell‐cycle arrest was completely blocked when the expressions of p21cip1 and p27kip1 were knocked‐down together. Taken together, these results suggest that the p21cip1‐ and p27kip1‐associated signaling pathways might be involved in the TB‐induced anti‐proliferation in KB cells. In vivo, TB (50 mg/kg, i.p.) significantly inhibited the KB tumor size. In these TB‐treated tumors, increases in the levels of p21cip1 and p27kip1 protein and decreases in the number of proliferating cell nuclear antigen‐positive cells and the microvessel density were observed. These findings demonstrate for the first time that TB might have potential to serve as a therapeutic tool in the treatment of oral cancer. Mol. Carcinog. © 2011 Wiley Periodicals, Inc.
FMR1 gray‐zone alleles: Association with Parkinson's disease in women?
Authors: Hall Deborah A. , Berry‐Kravis Elizabeth , Zhang Wenting , Tassone Flora , Spector Elaine , Zerbe Gary , Hagerman Paul J. , Ouyang Bichun , Leehey Maureen A. 
Published: May 2011

AbstractCarriers of fragile X mental retardation 1 repeat expansions in the premutation range (55–200 CGG repeats), especially males, often develop tremor, ataxia, and parkinsonism. These neurological signs are believed to be a result of elevated levels of expanded CGG‐repeat fragile X mental retardation 1 mRNA. The purpose of this study was to determine the prevalence of fragile X mental retardation 1 repeat expansions in a movement disorder population comprising subjects with all types of tremor, ataxia, and parkinsonism. We screened 335 consecutive patients with tremor, ataxia, or parkinsonism and 273 controls confirmed to have no movement disorders. There was no difference in fragile X mental retardation 1 premutation size expansions in the cases compared with controls. Eleven percent of the women with Parkinson's disease had fragile X mental retardation 1 gray‐zone expansions compared with 4.4% of female controls (odds ratio of 3.2; 95% confidence interval, 1.2–8.7). Gray‐zone expansions in patients with other phenotypes were not overrepresented in comparison with controls. Fragile X mental retardation 1 premutation range expansions are not more common in a mixed movement disorder population compared with controls. Our results, however, suggest that fragile X mental retardation 1 gray‐zone alleles may be associated with Parkinson's disease in women. © 2011 Movement Disorder Society
Protein tyrosine kinase signaling during oocyte maturation and fertilization
Authors: McGinnis Lynda K. , Carroll David J. , Kinsey William H. 
Published: May 2011

AbstractThe oocyte is a highly specialized cell capable of accumulating and storing energy supplies as well as maternal transcripts and pre‐positioned signal transduction components needed for zygotic development, undergoing meiosis under control of paracrine signals from the follicle, fusing with a single sperm during fertilization, and zygotic development. The oocyte accomplishes this diverse series of events by establishing an array of signal transduction pathway components that include a select collection of protein tyrosine kinases (PTKs) that are expressed at levels significantly higher than most other cell types. This array of PTKs includes cytosolic kinases such as SRC‐family PTKs (FYN and YES), and FAK kinases, as well as FER. These kinases typically exhibit distinct patterns of localization and in some cases are translocated from one subcellular compartment to another during meiosis. Significant differences exist in the extent to which PTK‐mediated pathways are used by oocytes from species that fertilize externally versus internally. The PTK activation profiles as well as calcium signaling pattern seems to correlate with the extent to which a rapid block to polyspermy is required by the biology of each species. Suppression of each of the SRC‐family PTKs as well as FER kinase results in failure of meiotic maturation or zygote development, indicating that these PTKs are important for oocyte quality and developmental potential. Future studies will hopefully reveal the extent to which these factors impact clinical assisted reproductive techniques in domestic animals and humans. Mol. Reprod. Dev. 78:831–845, 2011. © 2011 Wiley Periodicals, Inc.
Assessing muscle function of the male pelvic floor using real time ultrasound
Authors: Nahon Irmina , Waddington Gordon , Adams Roger , Dorey Grace 
Published: May 2011

AbstractAimsFollowing surgical removal of the prostate, there may be compromise to the function of the pelvic floor muscles causing continence problems. Assessing the pelvic floor muscles of male patients can be an invasive process that causes discomfort, making it worthwhile to evaluate alternatives to the conventional method of Digital Rectal Examination (DRE). Real time ultrasound (RTUS) has been used with female patients as an alternative to internal assessments. This paper examines the reliability and validity of assessing the male pelvic floor using abdominal RTUS.MethodsTwenty‐eight men (mean age 66.2) with a history of treatment for prostate cancer were recruited to the study. They were assessed via DRE and RTUS. Findings from the procedures were correlated for evaluation of validity, and the RTUS readings were repeated by different therapists to assess reliability.ResultsMeasurements on screen correlated moderately with DRE findings (r = 0.57, P = 0.002), and RTUS was found to have good reliability (Intra‐class Correlation Coefficient = 0.90). Continent men had more movement of the bladder wall on RTUS than those who were incontinent (P = 0.043). Scar tissue and an inability to maintain a moderately full bladder were found to cause the most difficulty in getting a complete picture of pelvic floor movement.ConclusionsRTUS can be used clinically to examine male pelvic floor function, and its use would be enhanced once it has been established by DRE that a true pelvic floor contraction is occurring. RTUS can give an indication of pelvic floor function as an alternative measurement method when DRE is contraindicated. Neurourol. Urodynam. 30:1329–1332, 2011. © 2011 Wiley‐Liss, Inc.
Principles and strategies for monitoring data collection integrity in a multi‐site randomized clinical trial of a behavioral intervention
Authors: Phillips‐Salimi Celeste R. , Donovan Stickler Molly A. , Stegenga Kristin , Lee Melissa , Haase Joan E. 
Published: May 2011

AbstractAlthough treatment fidelity strategies for enhancing the integrity of behavioral interventions have been well described, little has been written about monitoring data collection integrity. This article describes the principles and strategies developed to monitor data collection integrity of the “Stories and Music for Adolescent/Young Adult Resilience During Transplant” study (R01NR008583, U10CA098543, and U10CA095861)—a multi‐site Children's Oncology Group randomized clinical trial of a music therapy intervention for adolescents and young adults undergoing stem cell transplant. The principles and strategies outlined in this article provide one model for development and evaluation of a data collection integrity monitoring plan for behavioral interventions that may be adapted by investigators and may be useful to funding agencies and grant application reviewers in evaluating proposals. © 2011 Wiley Periodicals, Inc. Res Nurs Health 34:362–371, 2011
Polymer complexes: LIX. Supramolecular structural, spectral and thermal analysis of N‐[2‐(6‐aminopyridino)] acrylamide polymer complexes
Authors: El‐Sonbati Adel. Z. , Diab Mostafa. A. , Mohamed Ramadan. H. 
Published: May 2011

AbstractNovel ligand N‐[2‐(6‐aminopyridino)] acrylamide (APA) is prepared via amidation of 2,6‐diaminopyridine with acryloyl chloride in dry benzene as solvent. The ligand is characterized on the basis of elemental analysis, infra‐red (IR) analysis and 1H nuclear magnetic resonance. Metal–polymer complexes are reported and characterized based on elemental analysis, molar conductance, magnetic susceptibility measurements, IR, 1H nuclear magnetic resonance, electronic spectra and thermal analysis. The molar conductance of the polymer complexes in dimethylformamide corresponds to a 1:1/1:2 electrolyte, which is non‐electrolytic. IR spectra show that polyAPA is coordinated to the metal ions in a uni‐negatively bidentate manner with N, O donor sites of azomethine N and deprotonated enolic‐O. All the polymer complexes are of high spin type. On the basis of spectral studies an octahedral geometry may be assigned for Co(II) and Ni(II) polymer complexes. The thermal stabilities of the polymer complexes were studied and the activation energies of the degradation were calculated. Copyright © 2011 Society of Chemical Industry
Improving Latino, English language learners' reading fluency: The effects of small‐group and one‐on‐one intervention
Authors: Ross Sarah G. , Begeny John C. 
Published: May 2011

AbstractReading fluency is a critical yet commonly neglected component of early reading instruction. For the large percentage of English language learners (ELLs) who are struggling with or at risk for reading difficulties, there is insufficient research available to help educators implement time‐efficient interventions with these students. Using an experimental design common in field‐based research, the purpose of this study was to evaluate the differential effects of a one‐on‐one (1/1) and small‐group (SG) reading fluency intervention, both implemented with Spanish‐speaking ELLs. Using three forms of data‐analytic strategies (visual analysis, standard error of measurement, and randomization tests), results showed that nearly all students benefitted from the 1/1 intervention, and two students clearly benefitted from the SG intervention. Standardized reading assessments also demonstrated the positive impact of students receiving the interventions. Implications of these findings are primarily discussed with respect to school‐based practice. © 2011 Wiley Periodicals, Inc.
Synthesis of nonsymmetric chain end functionalized polyisobutylenes
Authors: Adekunle Olubummo , Herbst Florian , Hackethal Katharina , Binder Wolfgang H. 
Published: May 2011

AbstractWe present the synthesis of nonsymmetric α‐ω‐functionalized polyisobutylenes (PIBs) bearing different functional moieties on their chain ends. Thus, on one chain end either, a short tri‐ethylene oxide chain (TEO) or a phosphine oxide ligand is attached, whereas the other chain end is substituted by hydrogen bonding moieties (thymine/2,6‐diaminotriazine). The nonsymmetric PIBs were synthesized via living cationic polymerization using methyl‐styrene epoxide as initiator, followed by quenching reaction with 3‐bromopropyl‐benzene. Subsequent bromide/azide exchange and the use of the azide/alkyne click reaction allowed the synthesis of (a) (α)‐TEO‐(ω)‐thymine‐telechelic PIB (7a), (b) (α)‐triethyleneoxide‐(ω)‐triazine telechelic PIB (7b), and (c) (α)‐phosphinoxide‐(ω)‐thymine‐telechelic PIB (13) with molecular weights Mn ∼ 4000 g mol−1 and low polydispersities (Mw/Mn = 1.3). The chemical identity of the final structures was proven by extensive 1H NMR investigations and matrix‐assisted laser desorption/ionization‐mass spectroscopy (MALDI). The presented method for the first time offers a simple and highly versatile approach toward supramolecular nonsymmetric α‐ω‐functionalized PIB. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011
Metal‐free synthesis of novel biobased dihydroxyl‐terminated aliphatic polyesters as building blocks for thermoplastic polyurethanes
Authors: Tang Donglin , Noordover Bart A. J. , Sablong Rafael J. , Koning Cor E. 
Published: May 2011

AbstractUsing the organic compound 1,5,7‐triazabicyclo[4.4.0]dec‐5‐ene (TBD) as a catalyst for step‐growth polymerization, a series of well‐defined hydroxyl‐telechelic renewable aliphatic polyesters (including poly(1,3‐propylene adipate); poly(1,4‐butylene adipate); poly(1,12‐dodecylene sebacate); and poly(1,2‐dimethylethylene adipate), PDMEA) were synthesized and studied. PDMEA is a novel polyester, which has not been reported before. The results of 1H NMR and Matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry indicate that the polymers are fully hydroxyl terminated. From differential scanning calorimetry (DSC) thermograms, we found that the glass transition temperatures (Tg) of these polyesters are below −20 °C. Only a Tg but no melting peak is observed in the DSC curve of the novel PDMEA. This indicates that PDMEA, contrary to the other renewable polyesters, is totally amorphous. Furthermore, using hexamethylene diisocyanate and hexamethylene diamine, poly(ester urethane urea)s (PEUUs) based on PDMEA were successfully synthesized. The Tg of the prepared PEUUs is below 0 °C, and no melting behavior of the soft‐segment is observed. The PEUU, with a flow temperature of over 200 °C, thus behaves as an elastomer at room temperature. Its mechanical properties, such as a relatively low tensile E‐modulus (≈20 MPa) at room temperature and a sufficiently high strain at break (≈560%), make it suitable for use in, for example, biomedical applications. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011
Synthesis, characterization, and large two‐photon absorption cross‐sections of solid red‐emitting 1,4‐diketo‐3,6‐diphenylpyrrolo [3,4‐c]pyrrole/3,6‐carbazole/terfluorene copolymers
Authors: Zhang Baoliang , Zhang Haichang , Li Xiaojing , Li Wen , Sun Pingping , Yang Wenjun 
Published: May 2011

AbstractThe synthesis, one‐ and two‐photon absorption (TPA) and emission properties of three novel 1,4‐diketo‐3,6‐diphenylpyrrolo[3,4‐c]pyrrole (DPP)/3,6‐carbazole (Cz)/terfluorene (TF) copolymers are reported. The molar ratios of DPP versus TF are 15:85 (TCP15), 25:75 (TCP25), and 50:50 (TCP50) under Cz:(TF + DPP) = 1. Two distinguished one‐photon absorption and emission bands observed in solutions imply that the electronic states of Cz–DPP–Cz and Cz–TF–Cz are not well mixed and the energy transfer from TF segments to DPP units is incomplete. However, in film states, all three copolymers are monochromatic red emitting with the peak wavelengths at 617, 621, and 631 nm for TCP15, TCP25, and TCP50, respectively, indicating that the interchain interactions also have played an important role in the energy transfer. In two‐photon measurement, the copolymer solutions still exhibit two distinguished emission bands but the relative intensities at short‐wavelength region are obviously decreased, implying that Cz–TF–Cz segment is high one‐photon active but low TPA active, whereas Cz–DPP–Cz unit is low one‐photon active but high TPA active. All the copolymers show large δ over the range of measured wavelengths and the δ values of TCP15, TCP25, and TCP50 increase with DPP contents and are up to 530, 770, and 850 GM per repeating unit, respectively. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011
Imaging the domain structure of organic semiconductor films
Authors: McNeill Christopher R. 
Published: May 2011

AbstractState‐of‐the‐art solution‐processed organic field‐effect transistors typically use polycrystalline organic semiconductor thin films as the active layer. Although it is widely regarded that boundaries between polycrystalline domains are a likely source of charge trapping limiting charge carrier mobility, little is known about the detailed domain structure of such films. Furthermore, variations in local order particularly in conjugated polymer films are likely to further impede charge transport. In recent years a number of techniques have been exploited that are able to provide information regarding local domain orientation and molecular order in polycrystalline organic thin films. These techniques have provided new information regarding the nature of domain structure providing an opportunity to directly evaluate the influence of domain structure on device operation. This article aims to provide a timely review of the experimental approaches used to date and provide a perspective for future work. © 2011 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys, 2011
Structural consequences of ATP hydrolysis on the ABC transporter NBD dimer: Molecular dynamics studies of HlyB
Authors: Damas João M. , Oliveira A. Sofia F. , Baptista António M. , Soares Cláudio M. 
Published: May 2011

AbstractABC transporters are a large and important family of membrane proteins involved in substrate transport across the membrane. The transported substrates are quite diverse, ranging from monatomic ions to large biomolecules. Consequently, some ABC transporters are involved in biomedically relevant situations, from genetic diseases to multidrug resistance. The most conserved domains in ABC transporters are the nucleotide binding domains (NBDs), which form a dimer responsible for the binding and hydrolysis of ATP, concomitantly with substrate translocation. To elucidate how ATP hydrolysis structurally affects the NBD dimer, and consequently the transporter, we performed a molecular dynamics study on the NBD dimer of the HlyB ABC exporter. We have observed a change in the contact surface between the monomers after hydrolysis, even though we have not seen dimer opening in any of the five 100 ns simulations. We have also identified specific regions that respond to ATP hydrolysis, in particular the X‐loop motif of ABC exporters, which has been shown to be in contact with the coupling helices of the transmembrane domains (TMDs). We propose that this motif is an important part of the NBD‐TMD communication in ABC exporters. Through nonequilibrium analysis, we have also identified gradual conformational changes within a short time scale after ATP hydrolysis.
Site‐directed spin labeling electron paramagnetic resonance study of the ORF1 protein from a mouse L1 retrotransposon
Authors: Januszyk Kurt , Fleissner Mark R. , Atchabahian Lara , Shieh Fa‐Kuen , Altenbach Christian , Martin Sandra L. , Guo Feng , Hubbell Wayne L. , Clubb Robert T. 
Published: May 2011

AbstractLong interspersed nuclear element‐1 is a highly abundant mammalian retrotransposon that comprises 17% of the human genome. L1 retrotransposition requires the protein encoded by open reading frame‐1 (ORF1p), which binds single‐stranded RNA with high affinity and functions as a nucleic acid chaperone. ORF1p has been shown to adopt a homo‐trimeric, asymmetric dumbbell‐shaped structure. However, its atomic‐level structure and mechanism of RNA binding remains poorly understood. Here, we report the results of a site‐directed spin labeling electron paramagnetic resonance (SDSL‐EPR) study of 27 residues within the RNA binding region of the full‐length protein. The EPR data are compatible with the large RNA binding lobe of ORF1p containing a RNA recognition motif (RRM) domain and a carboxyl‐terminal domain (CTD) that are predicted from crystallographic and NMR studies of smaller fragments of the protein. Interestingly, the EPR data indicate that residues in strands β3 and β4 of the RRM are structurally unstable, compatible with the previously observed sensitivity of this region to proteolysis. Affinity measurements and RNA‐dependent EPR spectral changes map the RNA binding site on ORF1p to residues located in strands β3 and β4 of the RRM domain and to helix α1 of the CTD. Complementary in vivo studies also identify residues within the RRM domain that are required for retrotransposition. We propose that in the context of the full‐length trimeric protein these distinct surfaces are positioned adjacent to one another providing a continuous surface that may interact with nucleic acids.
Inheritance of resistance to Bacillus thuringiensis Cry1Ac toxin in Helicoverpa armigera (Hübner) (Lepidoptera: Noctuidae) from India
Authors: Kaur Paramjit , Dilawari Vinod Kumar 
Published: May 2011

AbstractBACKGROUND: The cotton bollworm, Helicoverpa armigera (Hübner), is one of the most serious insect pests of cotton. It has developed resistance to almost all groups of chemical insecticides because of their intensive use. The failure of insecticides to control H. armigera has been a strong incentive for the adoption of transgenic cotton (Bt cotton). However, the value of Bt could be diminished by widespread resistance development to Bt toxins in insect populations. Therefore, understanding the genetic basis of resistance is essential for developing and implementing strategies to delay and monitor resistance.RESULTS: A resistant strain designated as BM‐R was obtained from the cross of adults from Bathinda () and Muktsar (), Punjab, India, which showed the highest survival (60.68%) and LC50 value (1.396 µg mL−1 diet). Similarly, a laboratory‐maintained strain from Hoshiarpur, Punjab, showed maximum susceptibility to Cry1Ac toxin with the lowest LC50 value (0.087 µg mL−1), and was designated as HP‐S. The genetic purity of both strains was confirmed by RAPD profile analysis at each generation, and genetic similarity reached more than 90% after the third generation. Continuous maintenance of the resistant BM‐R strain on Cry1Ac resulted in an increase in LC50 from 0.531 µg mL−1 in F0 to 4.28 µg mL−1 in F14 and 7.493 µg mL−1 in F19, while the LC50 values for HP‐S larvae on diet without Cry1Ac increased to 0.106 and 0.104 µg mL−1, which lay within the fiducial limits of the baseline LC50 value. The mode of inheritance of resistance was elucidated through bioassay response of resistant, susceptible heterozygotes and backcross progeny to Cry1Ac incorporated in semi‐synthetic diet.CONCLUSION: Based on dominance, degree of dominance and backcross values, resistance was inferred to be polygenic, autosomal and inherited as a recessive trait. Copyright © 2011 Society of Chemical Industry
Nanoscale photoelectron ionisation detector based on lanthanum hexaboride
Authors: Zimmer C. M. , Schubert J. , Hamann S. , Kunze U. , Doll T. 
Published: May 2011

AbstractA nanoscale ioniser is presented exceeding the limitation of conventional photoionisation detectors. It employs accelerated photoelectrons that allow obtaining molecule specificity by the tuning of ionisation energies. The material lanthanum hexaboride (LaB6) is used as air stable photo cathode. Thin films of that material deposited by pulsed laser deposition (PLD) show quantum efficiency (QE) in the range of 10−5 which is comparable to laser photo stimulation results. A careful treatment of the material yields reasonable low work functions even after surface reoxidation which opens up the possibility of using ultraviolet light emitting diodes (UV LEDs) in replacement of discharge lamps.magnified imageSchematic diagram of a photoelectron ionisation detector (PeID) operating by an electron emitter based on the photoelectric effect of lanthanum hexaboride.