bluuebunny/crossref_metadata_2025_split huggingface dataset:
ChemInform Abstract: Grignard Additions to Cyclic Sulfamidate Imines: Synthesis of N‐Substituted Quaternary Sulfamidate Building Blocks.
Authors: Chang Stanley , Lee Ernest E. 
Published: October 2010

AbstractA simple method is developed for the addition of Grignard reagents (in either Et2O or THF) to cyclic sulfamidate imines to give 4‐disubstituted derivatives.
ChemInform Abstract: Green Synthesis of 1,8‐Dioxo‐octahydroxanthene Derivatives Using Catalytic Amount of H2SO4 in Water.
Authors: Wang Rui‐Hua , Cui Bin , Zhang Wen‐Tao , Han Guang‐Fan 
Published: October 2010

AbstractThe presented method has several advantages such as low cost, excellent yield and a simple work‐up procedure.
ChemInform Abstract: Synthesis of Aryl‐Substituted Pyrimidines by Site‐Selective Suzuki‐—Miyaura Cross‐Coupling Reactions of 2,4,5,6‐Tetrachloropyrimidine.
Authors: Hussain Munawar , Nguyen Thai Hung , Khera Rasheed Ahmad , Malik Imran , Zinad Dhafer Saber , Langer Peter 
Published: October 2010

AbstractSuzuki—Miyaura reactions of the tetrachloropyrimidine (I) allow a convenient synthesis of mono‐, di, tri‐, and tetraarylpyrimidines, which are not readily available by other methods.
ChemInform Abstract: Synthesis of Novel 3,7‐Dihydro‐purine‐2,6‐dione Derivatives.
Authors: Liu Gang , Reddy P. S. Murali Mohan , Barber Jack R. , Ng Shi Chung , Zhou Yuefen 
Published: October 2010

AbstractThree series of substituted xanthines (46 examples) with great structural diversity are synthesized for biological screening.
ChemInform Abstract: Cucurbitane‐Type Triterpenes with anti‐Proliferative Effects on U937 Cells from an Egyptian Natural Medicine, Bryonia cretica: Structures of New Triterpene Glycosides, Bryoniaosides A and B.
Authors: Matsuda Hisashi , Nakashima Souichi , Abdel‐Halim Osama Bashir , Morikawa Toshio , Yoshikawa Masayuki 
Published: October 2010

AbstractBioassay‐guided fractionation leads to the isolation of two new triterpene glycosides, bryoniaosides A (I) and B (II), along with several known structurally related substances.
ChemInform Abstract: Dibenzocyclooctadiene Lignans from Schisandra lancifolia and Their anti‐Human Immunodeficiency Virus‐1 Activities.
Authors: Yang Guang‐Yu , Fan Peng , Wang Rui‐Rui , Cao Jing‐Li , Xiao Wei‐Lie , Yang Liu‐Meng , Pu Jian‐Xin , Zheng Yong‐Tang , Sun Han‐Dong 
Published: October 2010

AbstractThree new dibenzocyclooctadienes (I), named schilancifolignans A‐C, are isolated together with several known lignans from leaves and stems of the title plant.
ChemInform Abstract: Microdroplets in Microfluidics: An Evolving Platform for Discoveries in Chemistry and Biology
Authors: Theberge Ashleigh B. , Courtois Fabienne , Schaerli Yolanda , Fischlechner Martin , Abell Chris , Hollfelder Florian , Huck Wilhelm T. S. 
Published: October 2010

AbstractReview: 228 refs.
Trends in survival for patients with metastatic soft‐tissue sarcoma
Authors: Italiano Antoine , Mathoulin‐Pelissier Simone , Cesne Axel Le , Terrier Philippe , Bonvalot Sylvie , Collin Françoise , Michels Jean‐Jacques , Blay Jean‐Yves , Coindre Jean‐Michel , Bui Binh 
Published: October 2010

AbstractBACKGROUND:The objective of this study was to determine whether the overall survival of patients with metastatic soft tissue sarcoma (STS) has improved over the last 20 years.METHODS:In total, 1024 patients who had synchronous metastatic (SM) STS or metachronous metastatic (MM) STS diagnosed between 1987 and 2006 were included prospectively in the French Sarcoma Group database after central histologic review. Four periods of diagnosis of metastatic disease were defined: P1, from 1987 to 1991 (n = 208); P2, from 1992 to 1996 (n = 287); P3, from 1997 to 2001 (n = 285); and P4, from 2002 to 2006 (n = 244). Patient characteristics were analyzed as prognostic factors by using a Cox model.RESULTS:The proportion of patients with SM, the interval between diagnosis and MM, and the clinical characteristics of the patients were similar across the 4 periods. Although there was no significant difference in the median overall survival (OS) from P1 through P2 (P1, 12.3 months; 95% confidence interval [CI], 9.9‐14.7 months; P2, 11.4 months; 95% CI, 9‐13.9 months), significant improvements were observed in the later periods (P3, 15 months; 95% CI, 11.8‐18.2 months; P4, 18 months; 95% CI, 15.3‐20.7 months; P = .029; log‐rank test). The 2‐year OS rate also increased throughout the study period from 28.1% during P1 to 38.7% during P4. On multivariate analysis, period of diagnosis, age, histologic subtype, time to metastatic recurrence, French Federation of Cancer Centers Sarcoma Group grade, and the number of metastatic sites were independent prognostic factors for OS.CONCLUSIONS:The current analysis revealed that the median OS of patients with metastatic STS had improved by 50% during the last 20 years. These data should be considered in the interpretation of results from ongoing and future STS trials. Cancer 2011. © 2010 American Cancer Society.
Experience with standardized thyroid fine‐needle aspiration reporting categories
Authors: Nga Min‐En , Kumarasinghe Marian P. , Tie Bibiana , Sterrett Gregory F. , Wood Benjamin , Walsh John , Nguyen Hue , Whyte Andrew , Frost Felicity A. 
Published: October 2010

AbstractBACKGROUND.A significant number of thyroid fine‐needle aspiration cytology (FNAC) cases yield inconclusive results. The recent National Cancer Institute guidelines and those published by other societies are important contributions to standardizing the diagnostic approach. Nevertheless, there are significant issues in the application of guidelines and the evaluation of their clinical efficacy. Data from individual departments can be useful in demonstrating the role of standardized reporting.METHODS.The authors followed 529 consecutive cases with inconclusive thyroid FNA results that were analyzed in a single laboratory in Western Australia. In that laboratory, standardized reporting in categories has been in place for a decade, and inconclusive cases have been subdivided into indeterminate and atypical groups. Follow‐up data was obtained for 341 indeterminate cases (17.2% of total thyroid FNA accessions) and for 188 atypical cases (9.5% of accessions).RESULTS.In total, 127 nodules with atypical results (67.6%) underwent surgical excision compared with 131 nodules with indeterminate results (38.4%;P< .0001). In 96 excised nodules with atypical results (75.6%), the excised specimens were identified as neoplastic compared with 61 excised nodules with indeterminate results (46.6%;P< .0001). In addition, 31 excised nodules with atypical results (24.4%) had a malignancy proven compared with 17 excised nodules with indeterminate results (13%;P< .05). In addition, 51 of 82 repeat FNAs (62.2%) among patients who had indeterminate results yielded a more specific diagnosis compared with 2 of 9 repeat FNAs (22.2%) among patients who had atypical results (P< .05).CONCLUSIONS.The routine subcategorization of patients who had inconclusive thyroid FNA reports into indeterminate and atypical groups resulted in statistically significant differences in the likelihood of neoplasia and malignancy. Patients who had indeterminate results were more likely to benefit from repeat FNAC than patients who had atypical results. The current results indicated that patients who fall into these 2 categories are likely to benefit from different clinical management protocols. Cancer Cancer Cytopathol 2010. © 2010 American Cancer Society.
Maternal plasma polychlorinated biphenyl levels in cynomolgus monkeys (Macaca fascicularis) affect infant social skills in mother–infant interaction
Authors: Nakagami Akiko , Koyama Takamasa , Kawasaki Katsuyoshi , Negishi Takayuki , Ihara Toshio , Kuroda Yoichiro , Yoshikawa Yasuhiro 
Published: October 2010

AbstractPolychlorinated biphenyls (PCBs) are endocrine disrupting chemicals that disturb normal development of embryonic brains. In the present study, we evaluated the relationship between maternal plasma PCB concentration and infant behavioral characteristics in mother–infant interactions. We grouped 20 pregnant cynomolgus monkeys (Macaca fascicularis) into higher and lower PCB exposure groups; monkeys in the higher PCB group had PCB concentrations above 15 pg/g, which is representative of natural exposure levels. Maternal PCB concentration correlated negatively with infant behaviors (approach, look, proximity, locomotion) at the age of 6 months (p < .05), when an increase in these behaviors should normally occur. These results suggest that maternal PCB exposure may affect the development of infant social behavior in cynomolgus monkeys. Furthermore, this study provides primate evidence to support observations of associations between behavioral and learning disabilities and prenatal exposure to PCBs in humans. © 2010 Wiley Periodicals, Inc. Dev Psychobiol 53: 79–88, 2011.
T cells acquire cell surface determinants of APC via in vivo trogocytosis during viral infections
Authors: Rosenits Kerstin , Keppler Selina J. , Vucikuja Smiljka , Aichele Peter 
Published: October 2010

AbstractTrogocytosis describes the transfer of surface determinants between immune cells and has been implicated in immune regulation. Most findings are based on in vitro studies since in vivo trogocytosis of immune cells is difficult to detect under physiological conditions. We used low frequencies of memory P14 T cells to demonstrate that T cells perform trogocytosis in vivo if in contact with APC pulsed with GP33‐peptide or expressing the antigen endogenously. Furthermore, in vivo trogocytosis of T cells is demonstrated during infections with lymphocytic choriomeningitis virus and vaccinia virus. Trogocytosis‐positive T cells revealed higher expression of activation marker and cytokines, showing a more activated phenotype compared to trogocytosis‐negative T cells.
Application of microchip CGE for the analysis of PEG‐modified recombinant human granulocyte‐colony stimulating factors
Authors: Park Eun Ji , Lee Kyung Soo , Lee Kang Choon , Na Dong Hee 
Published: October 2010

AbstractThe purpose of this study was to evaluate the microchip CGE (MCGE) for the analysis of PEG‐modified granulocyte‐colony stimulating factor (PEG‐G‐CSF) prepared with PEG‐aldehydes. The unmodified and PEG‐modified G‐CSFs were analyzed by Protein 80 and 230 Labchips on the Agilent 2100 Bioanalyzer. The MCGE allowed size‐based separation and quantitation of PEG‐G‐CSF. The Protein 80 Labchip was useful for PEG‐5K‐G‐CSF, while the Protein 230 Labchip was more suitable for PEG‐20K‐G‐CSF. The MCGE was also used to monitor a search for optimal PEG‐modification (PEGylation) conditions to produce mono‐PEG‐G‐CSF. This study demonstrates the usefulness of MCGE for monitoring and optimizing the PEGylation of G‐CSF with the advantages of speed, minimal sample consumption, and automatic quantitation.
Consistent MYC and FLT4 gene amplification in radiation‐induced angiosarcoma but not in other radiation‐associated atypical vascular lesions
Authors: Guo Tianhua , Zhang Lei , Chang Ning‐En , Singer Samuel , Maki Robert G. , Antonescu Cristina R 
Published: October 2010

AbstractAngiosarcoma (AS) is a distinct group of sarcomas characterized by upregulation of vascular‐specific receptor tyrosine kinases, including TIE1, KDR, TEK, and FLT1. In keeping with the clinical heterogeneity, gene‐expression profiling distinguishes two AS genomic clusters, which correlate with anatomical location and prior exposure to radiation. Furthermore, a high percentage of secondary AS, but not primary AS, shows distinct 8q24 chromosomal gains, due to MYC amplification. In this study, we mined the transcriptional output of 10 secondary and 11 primary AS to better define the dichotomy in the pathogenesis of these two clinical subsets. The oncogenic role of MYC was investigated further in secondary AS as well as in radiation‐induced atypical vascular lesions (AVL) and other radiation‐associated sarcomas. High‐level MYC amplification was found in 100% of secondary AS, but in none of the AVL or other radiation‐associated sarcomas. Coamplification of FLT4 (encoding VEGFR3) was identified in 25% of secondary AS, but not in other types. Our findings reinforce the distinct pathogenesis of AS subtypes, with MYC amplification being an early, but necessary event in secondary AS. Secondary genetic hits, such as FLT4 gene coamplification or KDR mutations, may play a role in tumor progression as well as potential therapeutic targeting. © 2010 Wiley‐Liss, Inc.
Synthesis and antifungal activity of novel 5‐substituted 4‐(1,3,4‐thiadiazol‐2‐yl)benzene‐1,3‐diols
Authors: Matysiak Joanna , Skrzypek Alicja , Niewiadomy Andrzej 
Published: October 2010

Abstract5‐Substituted (amine, alkyl, aryl, heterocyclic) 4‐(1,3,4‐thiadiazol‐2‐yl)benzene‐1,3‐ diols were synthesized, and their antifungal properties were examined. The compounds were obtained by the one‐pot reaction of sulfinylbis((2,4‐dihydroxyphenyl)methanethione) with hydrazides or thiosemicarbazides. Their structures were identified from elemental, IR, 1H NMR, and MS spectra analyses. The activities of the derivatives against five phytopathogenic fungi in vitro were measured. Moderate fungicidal effect of the compounds under consideration was found. © 2010 Wiley Periodicals, Inc. Heteroatom Chem 21:533–540, 2010; View this article online at wileyonlinelibrary.com. DOI 10.1002/hc.20645
Higher bone mineral density is associated with a decreased risk of colorectal adenomas
Authors: Nock Nora L. , Patrick‐Melin Aimee , Cook Marc , Thompson Cheryl , Kirwan John P. , Li Li 
Published: October 2010

AbstractBone mineral density (BMD) is a biomarker for cumulative exposure to multiple factors including estrogen, calcium, vitamin D and physical activity, which have all been independently associated with colorectal cancer. Furthermore, higher levels of BMD have been inversely associated with colorectal cancer risk, particularly in postmenopausal women. However, no prior studies have examined the potential association between BMD and colorectal adenomas, which are precursor lesions to most colorectal cancers. Therefore, we evaluated the association between BMD, which was measured using a whole body, dual‐energy X‐ray absorptiometry scan and colorectal adenomas in 167 patients who underwent colonoscopy screening. We found that patients in the highest tertile of total body BMD (>1.294 g/cm2) and in the middle tertile (≥1.167 to ≤1.294 g/cm2) compared to those with a total body BMD in the lowest tertile (<1.167 g/cm2) had a lower risk of colorectal adenomas (highest vs. lowest tertile: OR = 0.29 (0.10–0.84); middle vs. lowest tertile: OR = 0.26 (0.08–0.80); p‐trend = 0.02). Stratification by gender revealed that this association was more pronounced in women (highest (>1.280 g/cm2) vs. lowest (<1.130 g/cm2) tertile: OR = 0.08 (0.01–0.70); middle (≥1.130 to ≤1.280 g/cm2) vs. lowest tertile: OR = 0.15 (0.04–0.94); p‐trend = 0.02) even after excluding hormone replacement therapy users (highest (>1.295 g/cm2) and middle (≥1.132 to ≤1.295 g/cm2) vs. lowest (<1.132 g/cm2) tertile: OR = 0.17 (0.03–0.97); p‐trend = 0.04). Our results show, for the first time, that BMD is inversely associated with colorectal adenomas, particularly in women. Although additional larger, prospective studies are needed, our results suggest that BMD may be a biomarker for colorectal cancer precursor lesions.
Modelling dependence using skew t copulas: Bayesian inference and applications
Authors: Smith Michael S. , Gan Quan , Kohn Robert J. 
Published: October 2010

AbstractWe construct a copula from the skew t distribution of Sahu et al. (2003). This copula can capture asymmetric and extreme dependence between variables, and is one of the few copulas that can do so and still be used in high dimensions effectively. However, it is difficult to estimate the copula model by maximum likelihood when the multivariate dimension is high, or when some or all of the marginal distributions are discrete‐valued, or when the parameters in the marginal distributions and copula are estimated jointly. We therefore propose a Bayesian approach that overcomes all these problems. The computations are undertaken using a Markov chain Monte Carlo simulation method which exploits the conditionally Gaussian representation of the skew t distribution. We employ the approach in two contemporary econometric studies. The first is the modelling of regional spot prices in the Australian electricity market. Here, we observe complex non‐Gaussian margins and nonlinear inter‐regional dependence. Accurate characterization of this dependence is important for the study of market integration and risk management purposes. The second is the modelling of ordinal exposure measures for 15 major websites. Dependence between websites is important when measuring the impact of multi‐site advertising campaigns. In both cases the skew t copula substantially outperforms symmetric elliptical copula alternatives, demonstrating that the skew t copula is a powerful modelling tool when coupled with Bayesian inference. Copyright © 2010 John Wiley & Sons, Ltd.
Reprogramming the pluripotent cell cycle: Restoration of an abbreviated G1 phase in human induced pluripotent stem (iPS) cells
Authors: Ghule Prachi N. , Medina Ricardo , Lengner Christopher J. , Mandeville Matthew , Qiao Meng , Dominski Zbigniew , Lian Jane B. , Stein Janet L. , van Wijnen Andre J. , Stein Gary S. 
Published: October 2010

AbstractInduced pluripotent stem (iPS) cells derived from terminally differentiated human fibroblasts are reprogrammed to possess stem cell like properties. However, the extent to which iPS cells exhibit unique properties of the human embryonic stem (hES) cell cycle remains to be established. hES cells are characterized by an abbreviated G1 phase (∼2.5 h) and accelerated organization of subnuclear domains that mediate the assembly of regulatory machinery for histone gene expression [i.e., histone locus bodies (HLBs)]. We therefore examined cell cycle parameters of iPS cells in comparison to hES cells. Analysis of DNA synthesis [5‐bromo‐2′‐deoxy‐uridine (BrdU) incorporation], cell cycle distribution (FACS analysis and Ki67 staining) and subnuclear organization of HLBs [immunofluorescence microscopy and fluorescence in situ hybridization (FISH)] revealed that human iPS cells have a short G1 phase (∼2.5 h) and an abbreviated cell cycle (16–18 h). Furthermore, HLBs are formed and reorganized rapidly after mitosis (within 1.5–2 h). Thus, reprogrammed iPS cells have cell cycle kinetics and dynamic subnuclear organization of regulatory machinery that are principal properties of pluripotent hES cells. Our findings support the concept that the abbreviated cell cycle of hES and iPS cells is functionally linked to pluripotency. J. Cell. Physiol. 226: 1149–1156, 2011. © 2010 Wiley‐Liss, Inc.
Oncoprotein Cot1 represses kinase suppressors of Ras1/2 and 1,25‐dihydroxyvitamin D3‐induced differentiation of human acute myeloid leukemia cells
Authors: Wang Xuening , Studzinski George P. 
Published: October 2010

AbstractMetabolites and derivatives of vitamin D are well‐known inducers of monocytic differentiation, but the mechanistic basis for their action is not fully elucidated. Here we show that the product of protooncogene Cot1 represses the monocytic phenotype in human acute myeloid leukemia (AML) cells induced to differentiate by 1,25‐dihydroxyvitamin D3 (1,25D), even though the expression of cellular Cot1 increases early in the process of 1,25D‐induced differentiation. Interestingly, the expression of the two members of the Kinase Suppressor of Ras (KSR) family of molecular scaffolds, known to be positive regulators of Ras signaling and of 1,25D‐induced differentiation, increases in parallel with Cot1 in 1,25D‐treated cells. However, KSR1/2 are negatively regulated by Cot1, as determined by transfection of siCot1, and confirmed by a reverse effect of ectopic expression of Cot1. The effect of Cot1 in AML cells appears to be cell‐type specific, as previous reports in other cell types found KSR‐2 to be a negative regulator of Cot1, a reverse relationship. Also in contrast to findings in other cells, in AML cells Cot1 exerts negative control on the MAP kinase pathways, since siCot1 increases the levels of activated Raf1, p90RSK, JNK1, c‐jun, and p38, though not of MEK/ERK. These findings have implications for therapy of AML, since in AML cells active MAPKs hasten cell differentiation, and specific pharmacological inhibitors of Cot1 kinase activity have recently became available, thus making Cot1 a “druggable” target. J. Cell. Physiol. 226: 1232–1240, 2011. © 2010 Wiley‐Liss, Inc.
PET translates neurophysiology into images: A review to stimulate a network between neuroimaging and basic research
Authors: Giovacchini Giampiero , Squitieri Ferdinando , Esmaeilzadeh Mouna , Milano Amalia , Mansi Luigi , Ciarmiello Andrea 
Published: October 2010

AbstractIn the last decades there has been a progressive advance in the development of techniques able to explore in humans neurophysiologic and neurochemical processes. Positron emission tomography (PET) is a very powerful technique allowing to study a quite variable range of physiological and biochemical processes in the healthy subjects and in diseases. Apart from its capacity to provide pathophysiological information, PET is also important for the objective assessment of therapeutic efficacy. Initial studies were performed measuring cerebral metabolic rate for glucose (CMRglc) and cerebral blood flow (CBF), representing an indirect index of synaptic activity. The advent of receptor tracers allowed measuring other important physiological parameters, such as receptor occupancy, and endogenous release. In neuropsychiatric disorders, as Alzheimer disease, schizophrenia, epilepsy and Huntington disease, PET has been useful to elaborate hypothesis of the pathogenesis, to relate symptoms to biological variables and to study individuals at increased risk. The new concepts of neurovascular unit and default network, preferentially active at rest, can significantly change the approach of PET, with images reflecting a complex scenario, not merely limited to neural activity, but involving the activity of the entire neurovascular unit and the multifunctional role of astrocytes. To detect dysfunction of the dialog between glutamatergic neurons and astrocytes could lead to a better understanding of altered functional brain images. In this direction a professional network between PET researchers and basic scientists, could give a determinant improvement in the capability to understand the complex physiological and pathophysiological cerebral world. J. Cell. Physiol. 226: 948–961, 2011. © 2010 Wiley‐Liss, Inc.