bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparative in vitro evaluation of two different magnetic devices detecting the stability of osseo‐integrated implants
Authors: Geckili O. , Bilhan H. , Cilingir A. , Mumcu E. , Bural C. 
Published: January 2012

Geckili O, Bilhan H, Cilingir A, Mumcu E, Bural C. A comparative in vitro evaluation of two different magnetic devices detecting the stability of osseo‐integrated implants. J Periodont Res 2012; 47: 508–513. © 2012 John Wiley & Sons A/SBackground and Objective:  It is unknown whether the resonance frequency analysis (RFA) measurements made by two different magnetic resonance frequency analysers are comparable. This in vitro study was designed to compare the RFA measurements made by the two magnetic resonance frequency analysers and to evaluate the intra‐ and interobserver reliability of the magnetic devices.Material and Methods:  Thirty‐two implants were placed in four cow ribs. The RFA value of each implant was measured by five different examiners. The measurements were repeated five times, in both the buccal and mesial directions, for each implant at 2 h intervals, and the averages of registered implant stability quotient (ISQ) units were recorded as the buccal ISQ value and the mesial ISQ value for every implant.Results:  No statistically significant differences (p > 0.05) were observed between the RFA measurements made by the two magnetic devices. The intra‐observer reliability of both devices was excellent, whereas the interobserver reliability of the devices was poor.Conclusion:  The results of the RFA measurements of both tested devices overlap. Although both devices show excellent intra‐observer reliability, there are variations between the measurements of different examiners.
Life history, damage assessment and control of Trioza hopeae (Hemiptera: Psylloidea), a serious pest on Hopea odorata (Malvales: Dipterocarpaceae) in Vietnam
Authors: VU Nga Thi , EASTWOOD Rod , BURCKHARDT Daniel 
Published: January 2012

AbstractYoung plants of the commercially important timber species Hopea odorata Roxb. are seriously damaged by the psyllid Trioza hopeae Burckhardt & Vu in nurseries in South Central and Southern Vietnam. Farmers routinely use Class 1 toxic insecticides to control the psyllids but these pose serious health risks in the urban environment. As a precursor to developing a pest management strategy, we studied the life cycle of T. hopeae and trialed several control measures. Trioza hopeae is polyvoltine with overlapping generations peaking in mid wet season. Females laid approximately 135 eggs; however, there was a natural attrition rate of about 80% in first‐instar nymphs due to the nymph's inability to find a suitable feeding site. Feeding nymphs induced pit galls on young H. odorata leaves and were present on all trees examined during the study. The pre‐adult life cycle lasted approximately 13 days and adult life span approximately seven days. Several toxic insecticides were found to be effective for controlling T. hopeae, but a novel non‐toxic alternative was equally as effective. We discuss these methods and potential biological control measures.
Amount of airflow required for olfactory perception in laryngectomees: a prospective interventional study
Authors: Manestar D. , Tićac R. , Maričić S. , Malvić G. , Čorak D. , Marjanović Kavanagh M. , Prgomet D. , Starčević R. 
Published: January 2012

Clin. Otolaryngol. 2012, 37, 28–34Objectives:  To determine minimum airflow rate required for olfactory stimulation in successfully rehabilitated laryngectomised patients after learning the polite yawning technique (PYT) and to confirm the hypothesis that sense of smell is rehabilitated once the nasal airflow is re‐established.Design:  Prospective open interventional trial.Setting:  Tertiary academic hospital.Participants:  The study population comprised 100 laryngectomised patients. The control group consisted of 100 non‐laryngectomised patients of similar age and sex. Rhinomanometry was used to measure air flow in the right and left nostrils, respectively, while the Smell Diskettes Olfaction test (SDOT) was used to test each individual’s sense of smell.Main outcome measures:  The primary endpoint was increasing the airflow, while the secondary endpoint was improvement in the Smell Diskettes Olfaction test score after learning the polite yawning technique.Results:  The difference in the Smell Diskettes Olfaction test results before and after introducing the polite yawning technique was statistically significant (F = 53.077; P < 0.001). The number of accurately identified odours increased with each measurement. There was a significant difference among rhinomanometric measurements of airflow through the right (F = 65.002; P < 0.001) and left nostrils (F = 75.465; P < 0.001). Nasal airflow improved with each measurement. The minimum airflow required for olfactory stimulation in successfully rehabilitated patients was approximately 60 cm3/s. The control group had considerably better airflow in both nostrils than the laryngectomised group. The difference between the total number of rehabilitated (normosmic) patients (48%) in the laringectomised group and normosmic participants (56%) in the control group (z = 1.132; P = 0.129) was not statistically significant.Conclusion:  The number of odours identified by laryngectomised patients increased with the volume of nasal airflow. The number of patients with rehabilitated olfactory function approximated the percentage of normosmic individuals in the non‐laryngectomised population. These findings confirm the hypothesis that sense of smell is rehabilitated once the nasal airflow is re‐established.
Characterization of Glioma Stem Cells Through Multiple Stem Cell Markers and Their Specific Sensitization to Double‐Strand Break‐Inducing Agents by Pharmacological Inhibition of Ataxia Telangiectasia Mutated Protein
Authors: Raso Alessandro , Vecchio Donatella , Cappelli Enrico , Ropolo Monica , Poggi Alessandro , Nozza Paolo , Biassoni Roberto , Mascelli Samantha , Capra Valeria , Kalfas Fotios , Severi Paolo , Frosina Guido 
Published: January 2012

AbstractPrevious studies have shown that tumor‐driving glioma stem cells (GSC) may promote radio‐resistance by constitutive activation of the DNA damage response started by the ataxia telangiectasia mutated (ATM) protein. We have investigated whether GSC may be specifically sensitized to ionizing radiation by inhibiting the DNA damage response. Two grade IV glioma cell lines (BORRU and DR177) were characterized for a number of immunocytochemical, karyotypic, proliferative and differentiative parameters. In particular, the expression of a panel of nine stem cell markers was quantified by reverse transcription‐polymerase chain reaction (RT‐PCR) and flow cytometry. Overall, BORRU and DR177 displayed pronounced and poor stem phenotypes, respectively. In order to improve the therapeutic efficacy of radiation on GSC, the cells were preincubated with a nontoxic concentration of the ATM inhibitors KU‐55933 and KU‐60019 and then irradiated. BORRU cells were sensitized to radiation and radio‐mimetic chemicals by ATM inhibitors whereas DR177 were protected under the same conditions. No sensitization was observed after cell differentiation or to drugs unable to induce double‐strand breaks (DSB), indicating that ATM inhibitors specifically sensitize glioma cells possessing stem phenotype to DSB‐inducing agents. In conclusion, pharmacological inhibition of ATM may specifically sensitize GSC to DSB‐inducing agents while sparing nonstem cells.
Thermal Inactivation of Escherichia coli O157:H7 Inoculated at Different Depths of Non‐Intact Blade‐Tenderized Beef Steaks
Authors: Adler Jeremy M. , Geornaras Ifigenia , Belk Keith E. , Smith Gary C. , Sofos John N. 
Published: January 2012

Abstract:  Studies evaluated thermal inactivation of Escherichia coli O157:H7 inoculated at different depths of simulated blade‐tenderized non‐intact steaks. Fresh beef slices (0.3 or 0.6 cm thick) were stacked on top of each other to form 2.4 or 1.2 cm thick steaks. Steaks were blade‐tenderized and then inoculated with rifampicin‐resistant Escherichia coli O157:H7 (8 strain mixture; 4 log CFU/cm2) on the surface or between slices, vacuum‐packaged, and stored at 4 or –20 °C for 5 d before cooking. Steaks were cooked by pan‐broiling or roasting to a geometric center temperature of 60 °C. Frozen samples were either cooked from the frozen state or after thawing to approximately 4 or 25 °C. In steaks inoculated on the external surface and cooked by pan‐broiling, pathogen survivors recovered from thinner (1.2 cm) steaks were greater (P < 0.05) than those recovered from thicker (2.4 cm) steaks. Cooking steaks from a frozen state or after thawing (4 or 25 °C) did not (P≥ 0.05) affect extent of pathogen inactivation. Survivors after pan‐broiling of 2.4 cm thick steaks increased (P < 0.05) from 0.3 to 1.3 log CFU/cm2 for surface‐inoculated steaks to 2.5 to 3.2 log CFU/cm2 for samples inoculated at the center (1.2 cm depth). In comparison, overall thermal destruction of the pathogen in steaks cooked by roasting was less, and survivor counts were generally not different (P≥ 0.05) at each depth of inoculation. These data should be useful in development of lethality guidelines to ensure safe consumption of non‐intact meat products.Practical Application:  Results of this study should be useful for developing cooking guidelines, for foodservice establishments and consumers, to ensure safe consumption of non‐intact meat products.
Anterior mediastinal cystic seminoma
Authors: Hata Yoshinobu , Isobe Kazutoshi , Sato Fumitomo , Takahashi Shoji , Goto Hidenori , Tamaki Kazuyoshi , Wakayama Megumi , Shibuya Kazutoshi , Takagi Keigo 
Published: January 2012

AbstractMediastinal cystic seminoma is uncommon; only 17 cases have been reported, most diagnosed postoperatively, without recurrence on follow‐up, even without radiotherapy. Here, we report a mediastinal seminoma showing a unilocular cyst with enhancing thickened wall in computed tomography (CT) and septal structures in magnetic resonance imaging (MRI) in a 24‐year‐old man. Fluorodeoxyglucose (FDG) uptake was not significant. Histopathologically, seminoma cells were found scattered in the cyst wall. Twenty months after resection, the patient's quality of life is good, without chemotherapy or radiation. Cystic seminoma has a good prognosis and complete resection without adjuvant therapy might be sufficient for young patients of reproductive age.
Effect of interferon therapy on first and second recurrence after resection of hepatitis C virus‐related hepatocellular carcinoma
Authors: Sakae Masayuki , Kubo Shoji , Takemura Shigekazu , Sakata Chikaharu , Uenishi Takahiro , Kodai Shintaro , Shinkawa Hiroji , Urata Yorihisa , Ohata Kazunori , Kaneda Kazuhisa , Nishioka Takayoshi , Nozawa Akinori , Suehiro Shigefumi 
Published: January 2012

Aim:  Several investigators have shown that interferon (IFN) therapy can suppress the recurrence of hepatocellular carcinoma (HCC) after curative treatment. We investigated the effect of IFN therapy on the first and second HCC recurrence following hepatic resection of hepatitis C virus (HCV)‐related HCC.Methods:  Subjects included 166 patients who had undergone curative resection for a single HCV‐related HCC. We analyzed the outcome after initial hepatic resection and risk factors of a second HCC recurrence following treatment for the first HCC recurrence.Results:  Using multivariate analysis, a non‐sustained virological response (non‐SVR) was significantly associated with a high incidence of first HCC recurrence. The rate of second HCC recurrence tended to be higher in the non‐SVR group than in the SVR group. In the patients with recurrence of multiple tumors or who received non‐curative treatment for recurrent HCC, the second HCC recurrence rates were significantly higher. Multivariate analysis demonstrated that non‐curative treatment for first HCC recurrence was an independent risk factor for a second HCC recurrence. Among the patients who received curative treatment for their first HCC recurrence, the rates of second recurrence were significantly higher in the non‐SVR group than in the SVR group. Multivariate analysis also revealed that SVR was independently associated with prevention of a second HCC recurrence.Conclusions:  These results suggest that on first HCC recurrence, a curative treatment should be considered in order to prevent a second recurrence if possible. In addition, IFN therapy contributes to improved prognosis after curative treatment, even in patients with recurrent HCC.
Vehicle Replacement in the International Committee of the Red Cross
Authors: Pedraza‐Martinez Alfonso J. , Van Wassenhove Luk N. 
Published: January 2012

This article studies 4 × 4 vehicle replacement within the International Committee of the Red Cross (ICRC), one of the largest humanitarian organizations. ICRC policy sets the replacement of vehicles at 5 years or 150,000 km, whichever comes first. Using field data collected at the ICRC headquarters and national level we study the ICRC policy. Our results suggest that the organization can make considerable savings by adjusting its replacement policy. This study contributes to the area of logistics and transportation research in humanitarian operations.
Post‐transfusion survival of biotin‐labeled allogeneicRBCs in adult horses
Authors: Mudge Margaret C. , Walker Naomi J. , Borjesson Dori L. , Librach Fred , Johns Jennifer L. , Owens Sean D. 
Published: January 2012

BackgroundPost‐transfusion survival of allogeneicRBCs has been reported to be much shorter in horses than in other species. We hypothesized that post‐transfusion survival of biotinylated allogeneic equineRBCs would be greater than the survival previously reported from studies using radioactiveRBC‐labeling techniques.ObjectiveThe study objective was to determine post‐transfusion survival ofN‐hydroxysuccinimide (NHS)‐biotin‐labeled allogeneic equineRBCs transfused into adult horses.MethodsHorses were adults and included 5 donors and 5 recipients. All horses were blood‐typed, and donors were paired with recipients based upon blood type and crossmatch results. Donor blood was collected in a volume of 4 L into citrate phosphate dextrose adenine‐1 and stored for 24 hours, labeled withNHS‐biotin, and re‐infused into recipients. Post‐transfusion blood samples were collected at 15 minutes and at 1, 2, 3, 5, 7, 14, 21, 28, and 35 days. Biotin‐labeledRBCs were detected by flow cytometry using streptavidin–phycoerythrin. Post‐transfusion survival at 24 hours, lifespan, and half‐life of biotinylatedRBCs were determined.ResultsMean ± SDsurvival of biotinylatedRBCs at 24 hours post‐transfusion was 95 ± 24%; the mean lifespan of transfused allogeneicRBCs was 39 days based on calculation of a linear regression survival curve, and mean post‐transfusionRBChalf‐life was 20 days.ConclusionsPost‐transfusion survival of 24‐hour stored equine allogeneicRBCs was greater than previously reported but less than that observed for other companion animal species. Mechanisms for the relatively short post‐transfusion lifespan of allogeneic equineRBCs remain unknown and warrant further study.
Serum transthyretin concentration is decreased in dogs with nonthyroidal illness
Authors: Piechotta Marion , Raila Jens , Rick Markus , Beyerbach Martin , Hoppen Hans‐Otto 
Published: January 2012

BackgroundHypothyroidism in dogs is often difficult to diagnose owing to nonspecific clinical signs and laboratory test results that can be mimicked by nonthyroidal illness (NTI). Thyroxine (T4) circulates in blood mainly bound to T4‐binding globulin and, to a lesser degree, transthyretin (TTR) and albumin. The concentration of total T4 depends on the concentrations of these binding proteins.ObjectivesWe hypothesized that dogs with NTI and decreased serum total T4 concentrations would have decreased serum TTR concentrations. The objective of the study was to measure and compare serum TTR concentrations in healthy dogs, in dogs with NTI and low serum T4 concentrations, and in dogs with hypothyroidism.MethodsAssignment of dogs to 3 groups was based on physical examination and serum concentrations of T4 and TSH (mean ± SD): for healthy dogs (n = 13), T4 was 24.8 ± 3.6 nmol/L and TSH was 0.15 ± 0.08 μg/L; for dogs with NTI and low T4 (n = 20), T4 was 3.2 ± 3.0 nmol/L and TSH was 0.18 ± 0.13 μg/L; and for hypothyroid dogs (n = 19), T4 was 5.3 ± 4.3 nmol/L and TSH was 2.33 ± 1.90 μg/L). TTR concentrations in serum were determined semiquantitatively using western blot analysis.ResultsSerum TTR concentration (mean ± SD) was decreased in the dogs with NTI (24.8 ± 7.9 mg/L) compared with that of hypothyroid dogs (41.1 ± 21.4 mg/L, P = .0035). Differences were not found between TTR concentrations in clinically healthy dogs (33.3 ± 10.1 mg/L) and hypothyroid dogs or dogs with NTI.ConclusionsSerum TTR concentrations were significantly decreased in dogs with NTI and low T4 compared with concentrations in hypothyroid dogs. Additional studies should be done to determine if TTR concentrations can discriminate between dogs with NTI and low T4 and dogs with primary hypothyroidism.
Equine mammary gland disease with a focus on botryomycosis: A review and case study
Authors: Smiet E. , Grinwis G. C. M. , van den Top J. G. B. , Sloet van Oldruitenborgh‐Oosterbaan M. M. 
Published: January 2012

SummaryMammary gland problems occur incidentally in horses and one of the rarer conditions is botryomycosis (bacterial pseudomycosis, bacterial granuloma, staphylococcal pseudomycetoma). This article includes a short review of equine mammary gland problems inappropriate lactation, mastitis and neoplasia and botryomycosis, and additionally 2 clinical cases of botryomycosis of the udder resulting fromStaphyloccocus aureusinfection will be discussed. Both cases involved nonpregnant, nonlactating mares referred for chronic mammary inflammation with draining abcessation. In both mares, botryomycosis caused byS. aureuswas confirmed by histopathology and a bacterial culture. Both mares recovered fully after surgical hemimastectomy under general anaesthesia.
Neonatal respiratory distress and sepsis in the premature foal: Challenges with diagnosis and management
Authors: Johnson P. J. , LaCarrubba A. M. , Messer N. T. , DeClue A. E. 
Published: January 2012

SummaryThe presentation of a premature, neonatal foal affected with respiratory distress and seizures represents a difficult diagnostic and therapeutic challenge often best addressed by the provision of appropriate emergency care followed by prompt referral to a well‐equipped critical care facility. Veterinary management of the premature foal described in the accompanying report was complicated by the development of sepsis and pulmonary failure. The development of pulmonary emphysematous bullae was identified during the course of the foal's treatment and probably contributed to its clinical deterioration. Diagnostic imaging modalities that may be used for the diagnosis of respiratory distress in neonatal foals include thoracic radiography, ultrasonography, computed tomography (CT) and magnetic resonance imaging (MRI). Both CT and MRI require general anaesthesia. The likelihood of a successful outcome for the foal in this report might have been improved by the provision of urgent veterinary care and referral to the critical care facility earlier in the course of its management. Important early indicators of the need for urgent veterinary care in this case included the foal's prematurity, inability to stand, and the need to provide manual support to facilitate nursing from the mare's udder. Foals affected in this manner should warrant treatment with broad‐spectrum antimicrobials, circulating volume maintenance, immunoglobulin support, and the use of a nasogastric tube to facilitate nutritional support.
Preexposure Prophylaxis with Albumin-Conjugated C34 Peptide HIV-1 Fusion Inhibitor in SCID-hu Thy/Liv Mice
Authors: Stoddart Cheryl A. , Nault Geneviève , Galkina Sofiya A. , Bousquet-Gagnon Nathalie , Bridon Dominique , Quraishi Omar 
Published: January 2012

ABSTRACT

PC-1505 is a C34 peptide derived from the heptad repeat 2 region of HIV-1 gp41 conjugated to human serum albumin for sustained
in vivo
activity. One single preexposure dose of PC-1505 reduced viral RNA in HIV-1-infected SCID-hu Thy/Liv mice by 3.3 log
10
and protected T cells from virus-mediated depletion. In contrast, a single preexposure dose of Truvada reduced viral RNA by only 0.8 log
10
and was substantially less effective in preventing T cell depletion.
Klebsiella pneumoniae ST258 Producing KPC-3 Identified in Italy Carries Novel Plasmids and OmpK36/OmpK35 Porin Variants
Authors: García-Fernández Aurora , Villa Laura , Carta Claudio , Venditti Carolina , Giordano Alessandra , Venditti Mario , Mancini Carlo , Carattoli Alessandra 
Published: January 2012

ABSTRACT

A carbapenemase-resistant
Klebsiella pneumoniae
strain, clone ST258 producing KPC-3, was fully characterized. The entire plasmid content was investigated, thereby identifying plasmids of the IncFII
k
(two of them similar to pKPQIL and pKPN3, respectively), IncX, and ColE types, carrying a formidable set of resistance genes against toxic compounds, metals, and antimicrobial drugs and a novel iron(III) uptake system.
Tolerability and Safety of Primaquine in Papua New Guinean Children 1 to 10 Years of Age
Authors: Betuela Inoni , Bassat Quique , Kiniboro Benson , Robinson Leanne J. , Rosanas-Urgell Anna , Stanisic Danielle , Siba Peter M. , Alonso Pedro L. , Mueller Ivo 
Published: January 2012

ABSTRACT

Primaquine is currently the only drug available for radical cure of
Plasmodium vivax
and
P. ovale
liver infection stages, but limited safety data exist for children <10 years of age. Detailed daily assessments of side effects in glucose-6-phosphate dehydrogenase (G6PD)-normal children treated with 14 days of primaquine plus chloroquine (3 days;
n
= 252) or artesunate (7 days;
n
= 141) (0.5 mg/kg of body weight) showed that both treatments are well tolerated, do not lead to reductions in hemoglobin levels, and can thus safely be used in children 1 to 10 years of age.