bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of the STAT5B‐RARα fusion transcript in an acute promyelocytic leukemia patient without FLT3, NPM1, c‐Kit and C/EBPα mutation
Authors: Qiao Chun , Zhang Su‐Jiang , Chen Li‐Juan , Miao Kou‐Rong , Zhang Jian‐Fu , Wu Yu‐Jie , Qiu Hai‐Rong , Li Jian‐Yong 
Published: February 2011

AbstractT(15;17) is the most common chromosomal aberration in patients with acute promyelocytic leukemia (APL), leading to the formation of PML‐RARα fusion gene. In a small subset of patients with APL, the RARα gene is fused with different partners. Here, we report a rare APL case with STAT5B‐RARα fusion transcript. Cytomorphologic and immunophenotypic analyses showed typical features of APL. However, cytogenetic analysis showed normal karyotype, and interphase fluorescence in situ hybridization (FISH) showed PML‐RARα negative. Quantitative RT‐PCR also showed PML‐RARα negative but STAT5B‐RARα positive and sequencing analysis confirmed the result. Molecular markers including FLT3, NPM1, c‐Kit and C/EBPα mutation were all negative. To our knowledge, this is the first APL patient with STAT5B‐RARα in Chinese population and the fifth patient around the world according to published paper.
A new method for the visualization and quantification of internal skin elasticity by ultrasound imaging
Authors: Osanai Osamu , Ohtsuka Mayumi , Hotta Mitsuyuki , Kitaharai Takashi , Takema Yoshinori 
Published: February 2011

Background/purpose: Skin elasticity has been assessed previously only in the surface layer. We developed a new method that uses tissue strain imaging (TSI) technology, and the aim of this study was to test this new method to assess internal skin elasticity.Methods: Using a pressure device with a 12 MHz ultrasound transducer, constant and linear compressions were applied to the cheek skin of 35 volunteers (aged: 20–60 years). The elasticity of each layer (dermis, subcutaneous and muscle) was measured and analyzed using the TSI application software incorporated into the Toshiba Aplio™ XV ultrasound system. A skin tissue‐equivalent phantom, which is a block of material with the acoustic velocity (1530 m/s) of human skin, was collaboratively developed by OST Inc. This phantom was placed between the skin and the transducer as a reference material.Results: Skin elasticity was clearly visualized and quantified in each layer of the skin. Age‐dependent decreases in elasticity were determined in all layers of the skin. Among the three internal skin layers, the highest elasticity was determined in the subcutaneous layer followed by the muscle layer.Conclusion: These findings support the validity and sensitivity of the TSI method to assess the elasticity of various layers of skin.
Need for quality control for aEEG monitoring of the preterm infant: a 2‐year experience
Authors: Griesmaier Elke , Neubauer Vera , Ralser Elisabeth , Trawöger Rudolf , Kiechl‐Kohlendorfer Ursula , Keller Matthias 
Published: February 2011

AbstractAim:  To establish and apply a questionnaire for the evaluation of amplitude‐integrated electroencephalogram (aEEG) with respect to practicability and feasibility in the NICU, to reveal strategies for improvements in daily use and to investigate the level of staff know‐how with regard to performance and evaluation of bedside aEEG for the purpose of quality control.Methods:  Amplitude‐integrated electroencephalogram was routinely applied in preterm infants below 32 weeks of gestational age for a period of 2 years. Practicability, feasibility and the level of know‐how with regard to aEEG were assessed using a multiple‐choice questionnaire.Results:  Major problems in the use of aEEG in preterm infants were identified as time needed for placement and frequent readjustment of electrodes. Quality control showed the following problems: seizure activity was correctly diagnosed in 60.0%, discontinuous (DC) background in 45.5% and burst suppression (BS) in 41.8% of patients. Overall, BS and DC were the patterns most frequently interpreted incorrectly.Conclusion:  Amplitude‐integrated electroencephalogram is frequently used in neonatology. In preterm infants with several challenges, repeated theoretical and practical trainings as well as quality control are needed in order for aEEG to become a routinely used monitoring instrument in daily preterm care.
Blood tests and histological correlates in children with eosinophilic oesophagitis
Authors: Marlais M , Francis ND , Fell JME , Rawat DJ 
Published: February 2011

AbstractAim:  To determine the relationship between blood tests and oesophageal histology in Eosinophilic oesophagitis (EoE).Methods:  All children diagnosed with EoE at one hospital from 2000 to 2009 were considered for inclusion in this study. Three blood test results were analysed, blood eosinophil count, serum total immunoglobulin E (IgE) and radioallergosorbent tests (RAST) to common food allergens. Oesophageal histology was prospectively re‐reviewed, and mean eosinophil counts were enumerated. Blood test results were correlated with oesophageal eosinophil counts using Spearman’s rank test.Results:  Forty children (70% boys) were included in this study, median age at diagnosis 6.5 years (range 0–15). At the time of diagnosis, 78% of children had a raised blood eosinophil count, 90% had a raised serum total IgE and 83% had one or more positive RAST tests. The mean oesophageal eosinophil count was significantly correlated with both blood eosinophil count (p = 0.008) and serum total IgE level (p = 0.008).Conclusion:  This study shows that blood tests are often abnormal in children with EoE at the time of diagnosis. Our data demonstrate an association between histological abnormalities and blood test results in children with EoE.
Normal visual evoked potentials in preschool children born small for gestational age
Authors: Nilsson Josefin , Dahlgren Jovanna , Karlsson Ann‐Katrine , Grönlund Marita Andersson 
Published: February 2011

AbstractAim:  Previous studies have shown visual evoked potential (VEP) abnormalities in infants and animals born small for gestational age (SGA) compared with controls. The current exploratory study aims to investigate whether VEP abnormalities persist in older ages.Methods:  Pattern VEP latencies were obtained in 21 children (11 girls, 10 boys), born SGA and moderately preterm, at an average age of 5 years and 8 months. Fifty‐one children (24 girls, 27 boys, mean age of 5 years and 7 months), also born moderately preterm but with normal height and weight at birth, served as controlsResults:  Visual evoked potential results showed no significant differences in latency between children born SGA and controls born appropriate for gestational age (AGA) for either binocular stimulation, right eye or left eye stimulation.Conclusions:  Our findings do not indicate any differences in VEP latency at preschool age for children born SGA compared with children born AGA. The results may support previous studies, suggesting that children born SGA show accelerated neurophysiologic maturation during their first year of life and that previously delayed VEP latencies after catch‐up stay unchanged compared with controls.
Parental smoking, nasal resistance and rhinitis in children
Authors: Virkkula P , Liukkonen K , Suomalainen AK , Aronen ET , Kirjavainen T , Pitkäranta A 
Published: February 2011

AbstractAim:  To determine whether parent‐reported perennial rhinitis or objectively measured nasal resistance is more common in children from smoking families. To assess tonsillar size, nasopharyngeal airway and upper airway surgery frequency in children with smoking and non‐smoking parents.Methods:  Ninety‐five children (age 3–6 years, median 68 months) participated in this prospective cross‐sectional clinical study. History of nasal symptoms was obtained, and all underwent an ear–nose–throat examination, anterior rhinomanometry and a lateral cephalogram. Regular smoking by either parent and their child’s snoring was inquired about with a parental questionnaire. We compared children with a parental smoker and children without a parental smoker in the family.Results:  Smoking in the family led to increased risk for perennial rhinitis in the children up to 2.76‐fold (aOR, 95%CI 1.00–7.67), but with no difference in nasal resistance between children from smoking and non‐smoking households. Neither tonsillar size, nasopharyngeal airway nor upper airway surgery was associated with parental smoking.Conclusions:  Parental smoking is associated with symptoms of perennial rhinitis in children. The possible role of environmental tobacco smoke should be taken into account in parent counselling and in evaluation of children being treated for symptoms of rhinitis and nasal obstruction.
CD4+CD25high Treg cells in peripheral blood during remission and exacerbation of allergic asthma in children
Authors: Pumputiene Ingrida , Emuzyte Regina , Siaurys Almantas , Tamosiunas Vytas , Valiulis Arunas 
Published: February 2011

AbstractAim:  To determine the percentage of CD4+CD25high Treg cells in peripheral blood CD4+ T cells of allergic asthmatic children during disease remission and exacerbation.Methods:  Peripheral blood mononuclear cells (PBMC) and serum samples were collected from 6‐ to 11‐year‐old children with mild‐to‐moderate allergic asthma (n = 34) and from healthy controls (n = 15). CD4+CD25high T cells in PBMC were detected by flow cytometry. Total and specific IgE in serum were analysed by enzyme‐amplified chemiluminescence, and IL‐2 was measured by ELISA.Results:  There was no significant difference in CD4+CD25high T‐cell proportions between asthmatic children in exacerbation and remission as compared with controls. CD4+CD25high T‐cell percentages were not correlated with total and specific IgE. IL‐2 was elevated in both disease remission and exacerbation but did not correlate significantly with CD4+CD25high T‐cell percentages.Conclusion:  CD4+CD25high T‐cell proportion in the peripheral blood of total CD4+ T cells is not reduced in children with allergic IgE‐mediated asthma and does not differ between disease remission and exacerbation.
Intact recovery from early ‘acquired methylmalonic aciduria’ secondary to maternal atrophic gastritis
Authors: Weber‐Ferro Wencke , Hertzberg Christoph , Röder Helmut , Timme Katharina , Rossi Rainer 
Published: February 2011

AbstractAim:  A 6‐month‐old infant with severe hyporegenerative anaemia, muscular hypotonia and developmental delay is reported, and the metabolic, diagnostic and therapeutic implications of this case are discussed.Results:  Diagnostic work‐up disclosed vitamin B12 depletion with an elevated excretion of methylmalonic acid (MMA), but a normal plasma total homocysteine. MRI showed fronto‐temporal atrophy and a delay in myelinization. The boy’s disease was attributable to a maternal atrophic gastritis. After initiation of vitamin B12 supplementation, he quickly recovered regarding haematopoiesis and MMA excretion. His neurological development completely normalized during 18 months of follow‐up including assessment by Bayley scores.Conclusion:  As the majority of reported patients with this acquired form of methylmalonic aciduria show a persistent neurological deficit, early diagnosis of this condition is mandatory and should include sensitive markers of vitamin B12 depletion, namely MMA formation and plasma homocysteine.
Outcome in a paediatric cohort receiving ART in Addis Abeba, Ethiopia
Authors: Asfawesen GY , Solomie J , Bisirat T , Berhanu GM , Mebratu B , Rahlenbeck S 
Published: February 2011

AbstractAim:  To evaluate pediatric AntiRetroviral Treatment (ART) treatment in a resource‐limited setting.Methods:  Data from a paediatric ART cohort at the All African Leprosy and Rehabilitation Centre, Addis Abeba, were analysed. Outcome measures included survival, age, gender, WHO stage, weight, regimen and CD4 cell count.Results:  From April 2005 to December 2008, 482 children initiated ART. Median age was 5.9 years (IQR 3.7–9.8), and median CD4 cell count was 236 cells per mm3. Median follow‐up was 16 months (IQR = 6–24 month). Among those followed for 24 months (n = 188), 160 children were still receiving treatment, six had dropped out, eight had been transferred out, and 13 (7.5%) had died (4.0 deaths per 100 person‐years). Mortality was highest in the first 6 months resulting in a 4.3% mortality rate. Lower age and immune suppression (low CD4) were associated with mortality, while sex, drug regimen, tuberculosis at treatment initiation, application of stavudine and/or zidovudine were not.Conclusion:  ART treatment of children in resource‐limited settings is possible as demonstrated by low attrition. The high proportion of malnourished children and children admitted at late stage of the disease calls for attention. Efforts to enrol more children and at an earlier stage of the disease should be promoted.
Comparison of Efficacy of an In‐Office Whitening System Used with and without a Whitening Priming Agent
Authors: DA COSTA JULIANA , LUBISICH ERINNE , FERRACANE JACK , HILTON THOMAS 
Published: February 2011

ABSTRACTPurpose:  The purpose of this study was to compare the whitening efficacy of an in‐office whitening system with and without the whitening primer application and evaluate tooth and soft tissue sensitivity.Materials and Methods:  This was a randomized, split‐mouth design, single‐blinded, clinical study. Twenty‐five patients received a whitening priming agent (Power Swabs, Power Swabs Corporation, Beaverton, OR, USA) on right or left maxillary incisors prior to in‐office tooth whitening with Opalescence Boost (38% hydrogen peroxide; Ultradent Products, Inc., South Jordan, UT, USA). Color was evaluated with the Bleachedguide 3D Master (Vita Zahnfabrik, Bad Sackingen, Germany) and Vita Easyshade spectrophotometer (Vident, Brea, CA, USA), after 30 minutes, 1 day, and 15 days postwhitening. After each tooth color measurement, the subjects were asked to rate their tooth and soft tissue sensitivity experience using a visual analog scale (1–10 categories). Results were analyzed by two‐way repeated measurements analysis of variance/Tukey's (p < 0.05); Mann–Whitney rank sum test and Kruskal–Wallis.Results:  The teeth that were treated with the primer prior to tooth whitening did not show significant difference in ΔL*, Δa*, Δb*, ΔE* and delta shade guide from the teeth that were not treated with the primer, at the three time points evaluated (baseline versus 30 minutes after in‐office treatment, baseline versus 1 day, and baseline versus 15 days). None of the subjects experienced soft tissue sensitivity, and those who experienced tooth sensitivity said it was not noticeable after 15 days postwhitening.CLINICAL SIGNIFICANCEThe primer neither enhanced the whitening effect nor decreased tooth sensitivity when used before vital bleaching with Opalescence Boost (Ultradent Products, Inc., South Jordan, UT, USA). None of the subjects experienced soft tissue sensitivity, and some experienced transient tooth sensitivity.(J Esthet Restor Dent 23:97–105, 2011)
Establishment and characterization of a fibroblast cell line derived from Mongolian sheep
Authors: LIU Chang‐Qing , GUO Yu , GUAN Wei‐Jun , MA Yue‐Hui 
Published: February 2011

ABSTRACTThe Mongolian sheep ear marginal tissue fibroblast cell line (MSF32) from 32 samples was successfully established by using primary explants technique and cell cryoconservation technology. MSF32 cells were adherent, with a population doubling time of 28.2 h. Chromosome analysis showed that >90.2% of cells were diploid (2n = 54) prior to cell passage 4. Isoenzyme analyses of lactate dehydrogenase and malate dehydrogenase showed that the MSF22 cells had no cross‐contamination with other species. Tests for cell line contamination with bacteria, fungi, viruses and mycoplasmas were also negative. Plasmids encoding the fluorescent proteins pEGFP‐N3, pEGFP‐C1, pECFP‐N1, pECFP‐mito, pDsRed1‐N1 and pEYFP‐N1 were transfected into cells to study exogenous gene expression in the cells. The plasmid transfection efficiency was between 12.3% and 63.3%. Every index of the MSF32 cell line meets all the standard quality controls of American Type Culture Collection (ATCC). Not only has the genetic resources of the Mongolian sheep been preserved at the cell level, but also valuable materials had been provided for genome, postgenome and somacloning research.
Japanese consumer categorisation of beef into quality grades, based on Meat Standards Australia methodology
Authors: POLKINGHORNE Rod J. , NISHIMURA Takanori , NEATH Kate E. , WATSON Ray 
Published: February 2011

ABSTRACTThe objective was to evaluate the sensory categorisation of beef by Japanese consumers, based on Meat Standards Australia methodology. Various cuts of beef, with a wide range of quality (from Australian and Japanese cattle) and three cooking methods (grill, yakiniku, shabu shabu), were evaluated by 1620 Japanese consumers in Tokyo and Osaka. Consumers rated each sample for four sensory attributes (tenderness, juiciness, flavor and overall satisfaction), then selected one of four grades (unsatisfactory/2‐star, good everyday/3‐star, better than everyday/4‐star, and premium quality/5‐star), based on the quality of the beef within each cooking method. Meat quality scores, denoted as MQ4 scores (weighted combination of the four sensory attributes) were calculated from the Japanese consumer test results, to describe the Japanese consumer rating of beef. The distribution of the Japanese consumer MQ4 scores showed a clear distinction between grades, with the majority of scores being included within the boundaries of each grade. The MQ4 score allocated approximately 64% of the samples to their correct consumer grades. The MQ4 score showed potential to be used as a tool in developing and monitoring a consumer‐focused grading system that is able to predict Japanese consumer satisfaction of individual beef cuts prepared by different cooking methods.
Efficacy of probiotics from anaerobic microflora with prebiotics on growth performance and noxious gas emission in growing pigs
Authors: CHU Gyo Moon , LEE Shin Ja , JEONG Ho Sik , LEE Sung Sill 
Published: February 2011

ABSTRACTWe investigated the effect of probiotics from anaerobic microflora with prebiotics (synbiotics) on growth performance, noxious gas emission and fecal pathogenic bacteria populations in growing pigs. The basal diet, which contained approximately 25% corn, 24% whey, 12% wheat and 12% soybean meal, was supplemented alternatively with 0.15% antibiotics (US diet), prebiotics and 0.2% probiotics from anaerobic bacteria (BS diet), yeast (YS diet), mold (MS diet) or compounds (CS diet). One hundred and fifty pigs were fed an experimental diet for 15 days. Although the growth performance was not affected by supplemental synbiotics, the BS group showed higher dry matter and crude protein digestibility. The BS group decreased fecal ammonia and amine gas emissions, and increased fecal acetate gas emission compared with the US group. All synbiotics groups decreased in fecal propionate gas emission. Fecal Escherichia coli population was lower in the synbiotics groups than in the US group. Therefore, the results of the present study suggest that synbiotics exert similar effects with antibiotics on the nutrient digestibility and fecal microflora composition in growing pigs. Moreover, synbiotics can also decrease the fecal noxious gas emission in growing pigs.
Comparison of feeding systems: feed cost, palatability and environmental impact among hay‐fattened beef, consistent grass‐only‐fed beef and conventional marbled beef in Wagyu (Japanese Black cattle)
Authors: SITHYPHONE Khounsaknalath , YABE Mitsuyasu , HORITA Hiroshi , HAYASHI Keisuke , FUMITA Tomiko , SHIOTSUKA Yuji , ETOH Tetsuji , EBARA Fumio , SAMADMANIVONG Olavanh , WEGNER Jochen , GOTOH Takafumi 
Published: February 2011

ABSTRACTThe objective of this article is to compare feed cost, palatability and environmental impacts among feeding systems of high concentrate (HC), high hay (HH) and grass‐only‐fed (Gof) groups. Feed cost was the sum of costs paid for feed intake times the price of feed per kilogram. Palatability was measured by a panel taste test using HH and Gof beef and analyzed for differences. Environmental impacts were calculated based on 1 kg of Japanese beef yield of CO2 equivalents (eq) and animal end weights at each feeding stage. Results showed that the HH and Gof feeding systems could significantly reduce feed costs by approximately 60% and 78%, respectively, from the HC. In the panel taste test, 50% and 47.50% of panelists indicated that HH beef was ‘extremely delicious’ and ‘acceptable,’ respectively, while 15% indicated that Gof beef was ‘extremely delicious’; 62.50% indicated that Gof beef was ‘acceptable.’ Environmental impacts of each feeding system in terms of CO2 equivalents (eq) were 9.32, 6.10 and 2.04 tonnes of eq for the HC, HH and Gof, respectively. The HH was an economical system that produced moderate impacts on the environment and had impressive taste.
Tissue distribution of albumin‐glucose advanced glycation end products administrated to chickens
Authors: KITA Kazumi 
Published: February 2011

ABSTRACTGlycation (Maillard reaction) starts from non‐enzymatic amino‐carbonyl reaction binding carbonyl group of reducing sugars to amino group of amino acids. The amino‐carbonyl reaction leads to the formation of the stable Amadori product, which ultimately forms advanced glycation end products (AGE). Acceleration of glycation during hyperglycemia increases the production and accumulation of AGE, which is implicated in the gradual development of diabetic complications in diabetes mellitus. Avian species offers the great advantage of providing animal models for diabetology because high blood glucose concentration should accelerate to generate high concentration of AGE. In the present study, radioactive AGE was prepared from chicken serum albumin and 14C‐glucose. Radioactive AGE was administrated to examine the tissue distribution of AGE in chickens. Administration of radioactive AGE into the circulation of chickens revealed that AGE accumulated into specific tissues, liver, spleen and kidney, which have the function for clearing exogenous substances and endogenous metabolic waste products.
Evidence for a black hole in the X-ray transient XTEJ1859+226
Authors: Corral-Santana J. M. , Casares J. , Shahbaz T. , Zurita C. , Martínez-Pais I. G. , Rodríguez-Gil P. 
Published: February 2011

Abstract
We present the results of time-resolved optical photometry and spectroscopy of the X-ray transient XTEJ1859+226 (V406Vul). Photometric observations taken during 2000 and 2008 reveal the presence of the secondary star's ellipsoidal modulation. Further photometry obtained in 2010 shows the system ≃1mag brighter than its quiescence level and the ellipsoidal modulation diluted by strong flaring activity. Spectroscopic data obtained with the 10.4-m GTC in 2010 reveal radial velocity variations of ∼500kms−1 over 3h. A simultaneous fit to the photometry and spectroscopy using sinusoids to represent the secondary star's ellipsoidal and radial velocity variations, yields an orbital period of 6.58 ± 0.05h and a secondary star's radial velocity semi-amplitude of K2= 541 ± 70kms−1. The implied mass function is f(M) = 4.5 ± 0.6M⊙, significantly lower than previously reported but consistent with the presence of a black hole in XTEJ1859+226. The lack of eclipses sets an upper limit to the inclination of 70° which yields a lower limit to the black hole mass of 5.42M⊙.