bluuebunny/crossref_metadata_2025_split huggingface dataset:
Occupational methacrylate and acrylate allergy – cross‐reactions and possible screening allergens
Authors: Aalto‐Korte Kristiina , Henriks‐Eckerman Maj‐Len , Kuuliala Outi , Jolanki Riitta 
Published: August 2010

Background. Acrylic resin monomers, especially acrylates and methacrylates, are important occupational allergens. Aims. To analyse patterns of concomitant patch test reactions to acrylic monomers in relation to exposure, and to suggest possible screening allergens. Patients/methods. We reviewed the patch test files for the years 1994–2009 at the Finnish Institute of Occupational Health for allergic reactions to acrylic monomers, and analysed the clinical records of sensitized patients. Results. In a group of 66 patients allergic to an acrylic monomer, the most commonly positive allergens were three methacrylates, namely ethyleneglycol dimethacrylate (EGDMA), 2‐hydroxyethyl methacrylate (2‐HEMA) and 2‐hydroxypropyl methacrylate (2‐HPMA), and an acrylate, namely diethyleneglycol diacrylate (DEGDA). The patterns of concomitant reactions imply that exposure to methacrylates may induce cross‐reactivity to acrylates, whereas exposure to acrylates usually does not lead to cross‐allergy to methacrylates. Screening for triethyleneglycol diacrylate (TREGDA) in the baseline series was found to be useful, as 3 of 8 patients with diagnosed occupational acrylate allergy might have been missed without the screening. Conclusions. A short screening series of four allergens, EGDMA, DEGDA, 2‐HPMA and pentaerythritol triacrylate (PETA), would have screened 93% of our 66 patients; each of the remaining 5 patients reacted to different acrylic monomer(s).
Erythrocytosis and Hypernephroma
Authors: Thorling Eivind B. , Ersbak JØrgen 
Published: August 2010

The results of a study on the content of erythropoietic‐stimulating factor in the serum, urine and extracts from tumour tissue and normal renal tissue from a patient with erythrocytosis and hypernephroma of the left kidney are reported. Extracts from the solid parts of the tumour showed a high concentration of this factor. It seems reasonable to assume that this factor, which is presumed to be responsible for the patient's erythrocytosis, was produced by the tumour cells.Increased concentrations of erythropoietic‐stimulating factor were also revealed in the serum and urine after operation. Analyses of the metastases were not performed, but itseems reasonable to assume that these also produced an erythropoietic‐stimulating factor.
Glucose Metabolism in Normal Erythrocytes. I. Kinetics of the Hexokinase Reaction in Intact Cells
Authors: Garby Lars , de Verdier Carl‐Henric 
Published: August 2010

A method has been developed for determination of erythrocyte glucose consumption at a wide range of glucose concentrations. The method is based on paper chromatographic separation and quantitation of C14‐glucose and its labelled metabolites after incubation of intact cells.The glucose consumption was maximal at glucose concentrations above 0.4 mM.The inhibitory effect of inosine was investigated and found to be delayed, indicating an indirect action of this compound.The presence of EDTA in the medium increased the glucose consumption markedly, three to four times at 5–10 mM, whereas magnesium and calcium had no effect. EDTA removed easily exchangeable erythrocyte calcium during these conditions, but did not significantly penetrate the red cell membrane.Mercaptoethanol increased the glucose consumption, indicating the presence of potentially active SH‐groups on hexokinase in the intact cell.
Normal and Post‐Exercise Plasma Transfusion in Patients with Haemophilia A and von Willebrand's Disease
Authors: Ikkala Eero , Myllylä Gunnar , Nevanlinna H. R. 
Published: August 2010

Normal and post‐exercise plasma from the same donor was transfused into seven patients (into one patient twice) with haemophilia A and two patients with von Willebrand's disease. After post‐exercise plasma administration, on the average twice as much factor VIII activity was recovered in the patients than after normal plasma. Factor VIII levels after post‐exercise plasma transfusions, however, were clearly lower than the expected levels calculated from the in vitro activity of the transfused plasmas, especially when recalcification assay was used. Too high factor VIII values of post‐exercise plasmas in this assay system were due to some activation products, which clearly had less effect on the results of cephalin‐kaolin assay. The better in vivo effect of post‐exercise plasmas is regarded as an additional proof of real factor VIII increase during muscular exercise and the possibilities for the therapeutic use of post‐exercise plasmas are shortly discussed.In two patients with von Willebrand's disease the primary factor VIII response was comparable with that of A‐haemophiliacs and the bleeding time response better after post‐exercise plasmas, but no difference was observed in the secondary factor VIII response.
Experimental and Clinical Studies on AMCA, the Antifibrinolytically Active Isomer of p‐Aminomethyl Cyclohexane Carboxylic Acid
Authors: Andersson Lennart , Nilsson Inga Marie , Niléhn Jan‐Erik , Hedner Ulla , Granstrand Bengt , Melander Bengt 
Published: August 2010

1. Amino methyl cyclohexane carboxylic acid is a potent inhibitor of plasminogen activation. A preparation of the active isomer, AMCA, was found to be 6–8 times more potent than ε‐ACA and to have an inhibitory effect demonstrable in a concentration of 10‐4M. AMCA had no antiplasmin effect in concentrations up to 0.15 M.2. In high concentrations, 1 mg per ml blood, AMCA prolonged the thrombin time, but it had no other effect on the coagulation mechanism.3. Metabolic studies showed that the half‐life of AMCA in serum was short, about 1–2 hours. It was rapidly excreted in unchanged form in the urine and 90 per cent of a single intravenous dose of AMCA was excreted from the urine within 24 hours.4. AMCA was given to 50 subjects, including 20 with increased fibrinolytic activity and/or bleeding symptoms. It proved an effective antifibrinolytic agent and stopped bleeding caused by general or local fibrinolysis. Judging from in vivo tests the inhibiting effect of AMCA was about 7 times that of ε‐ACA. – Dizziness occurred in 6 cases, but no other side reactions or toxic effects of the drug were observed.5. AMCA is recommended as a useful clinical fibrinolytic inhibitor. A dosage of 0.015–0.020 g per kg bodyweight given intravenously every 4–6 hours appears to be adequate. For oral therapy doses of 0.05–0.1 g per kg bodyweight are required every 4–6 hours.
The Periodic Acid‐Schiff Reaction in Neutrophil Leukocytes in Chronic Myeloproliferative Diseases A microspectrophotometric study
Authors: Gahrton Gösta 
Published: August 2010

Microspectrophotometric quantification of the PAS reaction has been carried out in about 4500 individual mature neutrophil leukocytes from 20 normal persons, and from 15 patients with chronic myelocytic leukaemia (CML), 18 patients with myeloid metaplasia, and 17 patients with polycythaemia vera (PV).The reaction is considered to be a quantitative measure of the amount of PAS reactive material (PASMa) in neutrophils, most of it probably glycogen. Neutro‐phils from CML had a mean amount of PASMa that was 39 per cent below normal, the frequency distribution of PASMa per cell being approximately normal in individual cases. Neutrophils from myeloid metaplasia usually had elevated mean amounts of PASMa and considerably elevated variance between cells within subjects. Neutrophils from PV had a mean amount of PASMa that was 33 per cent above normal, with a highly augmented variance between cells within subjects. There was no correlation between the number of nuclear lobes and the amount of PASMa per cell in any diagnostic group. The quantified PAS reaction is considered to be of diagnostic importance for the differentiation of CML and myeloid metaplasia.
The Relationship Between Duodenal Mucosal Iron Concentration and Iron Absorption in Human Subjects
Authors: Allgood J. William , Brown Elmer B. 
Published: August 2010

Non‐haem iron concentrations of duodenal mucosa obtained by peroral capsule biopsy were determined in normal, iron‐deficient, and iron‐overloaded human subjects. Intestinal iron concentrations of normal subjects did not differ significantly from those of the iron‐deficient or iron‐overloaded subjects. No significant variation could be detected in iron concentration of mucosa obtained from different anatomical segments of duodenums removed at autopsy. Intestinal non‐haem iron concentrations showed no significant correlation with simultaneous measurements of serum iron concentration nor with radioiron absorption in a group of 25 patients (including normal, iron‐deficient, and iron‐overloaded subjects). Radioiron absorption was significantly correlated with severity of anaemia but not with serum iron concentration or per cent transferrin saturation.Failure to confirm previous studies in animals suggests that species differences exist in the regulation of gastrointestinal iron absorption and points up the need for additional studies of the control of iron absorption in man.
A Swedish Family with Haemolytic Anaemia, Heinz Bodies and an Abnormal Haemoglobin
Authors: Svensson Björn , Strand Lars 
Published: August 2010

A hereditary, haemolytic anaemia with Heinz‐body production has been found in four members of a Swedish family, presenting an abnormal haemoglobin according to various electrophoretic methods. Clinical and laboratory data indicate that this disease belongs to the group of Heinz‐body producing haemoglobinopathies.There are certain differences from related types of haemoglobinopathies, e.g. in terms of electrophoretic mobility and methaemoglobin production.Apparently this type of haemoglobin‐defect has not previously been described, for which reason we suggest the designation Haemoglobin Borås.
The Activity of Carbonic Anhydrases B and C from Human Erythrocytes and the Inhibition of the Enzymes by Copper
Authors: Magid E. 
Published: August 2010

The catalytic activity of two isolated forms of human erythrocytic carbonic anhydrase, HCAB and HCAC, was studied at 1.6° C by means of a pH‐stat method.80 per cent of the activity of the carbonic anhydrase with high specific activity (HCAC) was inhibited in the presence of 1.6 μM CuSO4, whilst the activity of the form with low specific activity (HCAB) was the same as that in a reaction medium which contained no inhibiting metallic ions. A chelating agent (EDTA), which eliminated the effect of CuSO4, had no effect on the enzyme activity in reaction media in which the concentration of the ions of heavy metals was below a critical level.The fact that HCAC is inhibited more effectively by copper than HCAB, was utilized in a procedure by means of which the activity of HCAC and HCAB was determined separately in solutions which contained both enzymes.The same procedure was used to determine the enzyme content of the erythrocytes, on the assumption that HCAC and HCAB in haemolysates had the same specific catalytic activity and were inhibited by CuSO4 to the same extent as in preparations of the isolated forms of carbonic anhydrase which were used in the investigations. On the basis of results from investigations of the blood from 24 donors it was calculated that HCAC comprises 80 per cent of the activity and about 20 per cent of the carbonic anhydrase concentration in red cells.The total carbonic anhydrase activity in erythrocytes from 5 new‐born infants was about one tenth of that in the erythrocytes from adults.A high erythrocytic carbonic anhydrase activity in 2 patients with untreated pernicious anaemia was found to be the result of a parallel increase in the activity of HCAB and HCAC.
Hereditary Thrombocytopenia
Authors: Myllylä G. , Pelkonen R. , Ikkala E. , Apajalahti J. 
Published: August 2010

Hereditary thrombocytopenia in three families is reported. The defect was obviously transmitted as an autosomal dominant characteristic in two of these. The thrombocytopenia was moderate and the bleeding symptoms were mild but of life‐long duration. Platelet ‐ adhesiveness in vivo and in vitro, clot‐retraction, ADP‐induced aggregation, and platelet factor 3 activity–were normal in all patients studied. The survival of autologous 51Cr‐labelled platelets was at the lower limit of normal or slightly shortened.In the third family autosomal recessive inheritance seemed probable. The bleeding tendency appeared at the age of 9 to 34 years with severe symptoms which rapidly led to death in four of the five patients. Two of the patients showed pancytopenia and this family probably presents a variant of constitutional hypoplastic anaemia. The functional parameters of platelets and the platelet survival in one of the patients were identical with those in the other two families.
Immunoglobulin Changes in the Course of Infectious Mononucleosis
Authors: WOLLHEIM FRANK A. 
Published: August 2010

The levels of γG, γA and γM have been determined by the Oudin tube method in 26 male and 19 female patients during the course of I.M. A control group consisting of 32 male and 27 female healthy young adults were also analysed.All classes of immunoglobulins were elevated already during the earliest stage of clinically manifest infectious mononucleosis (I.M.), indicating a long incubation period. The highest levels of γG were observed from 10 to 30 days after onset of fever (average 170 per cent of the normal mean), and a slow return towards normal levels took place. Peak levels for γA (163 per cent) occurred simultaneously, but a somewhat more rapid return to normal levels was evident. Increased levels of γM were a constant feature of acute I.M. and the highest levels (300 per cent) were observed from 1 to 20 days after onset of the illness. During convalescence a rapid return towards normal levels was observed, which seemed to parallel the disappearance of Downey cells.Whereas no sex difference was present among the I.M.‐patients γM‐levels were higher in the female controls.
Iron‐Deficient Erythropoiesis in Non‐Anaemic Young Men
Authors: Isager Henrik 
Published: August 2010

As a link in a study on the occurrence of iron deficiency in connection with adolescence a blind test, using iron and placebo, was performed on army recruits. Changes in haematocrit, serum iron, and transferrin in iron‐treated and placebo‐treated subjects were compared. A significant response to iron medication was found in respect to haematocrit (increase) and transferrin (decrease). The haematocrit response to iron was most marked in those subjects who had shown haematocrit values at the upper end of the normal range prior to the test. Comparison of pre‐treatment haematocrit values and transferrin revealed that persons having haematocrit values in the range 47–52% showed significantly higher transferrin values than the remainder of the subjects.It is assumed that iron‐deficient erythropoiesis may develop in young men exposed to a particularly vigorous hormonal stimulation of the erythropoiesis.
The Use of 75Se‐Methionin for the in vivo Study of Platelet Kinetics
Authors: Najean Y. , Ardaillou N. 
Published: August 2010

Platelet kinetics were studied using 75Se‐methionin as an in vivo tracer in normal and aplasic rats and in normal man and in patients suffering from bone marrow inadequacy, thrombocytosis, thrombocytopenia due to hyperdestruction.Results are compared with those obtained with 51Cr‐labelling.The method is found useful to measure both platelet production and autologous platelet life span in thrombocytopenic patients.
Some Effects of pH and Temperature upon Normal Human Red Cell Adenosine Triphosphate
Authors: Scott G. L. , Oates M. , Grimes A. J. 
Published: August 2010

Red cell ATP levels were determined on the fresh blood of 58 normal subjects using an enzymic method. Values were obtained in the range 80 to 140 μmoles of ATP per 100 ml. of packed red cells.Conditions for obtaining reliable fresh blood ATP values are reported together with the effects of different anticoagulants.It was confirmed that red cell ATP is pH‐sensitive. Thus, at pH values below 7.6–7.7 (25° C.) it was stable but at values above this range it disappeared from the red cells. Kinetic studies of red cell ATP at different temperatures and pH values are described.
Glycogen in Human Peripheral Blood Leukocytes
Authors: Abul‐Fadl Y. , Scott Robert B. 
Published: August 2010

The influence of female sex hormones on leucocyte glycogen and leucocyte alkaline phosphatase (LAP) was studied. Leucocyte glycogen was measured with the anthrone reagent on suspensions of peripheral blood leucocytes containing chiefly neutrophiles. LAP was measured histochemically. Normal adult pre‐menopausal females were shown to have significantly greater leucocyte glycogen levels and LAP score than males. Post‐menopausal females had intermediate glycogen levels and no elevation of LAP. During the menstrual cycle, there was a slight rise of leucocyte glycogen and LAP in the midportion of the cycle. In pregnancy LAP rose significantly above normal in the fourth month and leucocyte glycogen in the sixth month. Both had returned to normal 6 weeks after delivery. Leucocytes from cord blood of newborns had elevated LAP scores but normal glycogen levels. In normal females on anovulatory drugs LAP was elevated while leucocyte glycogen was normal. Patients receiving estrogens therapeutically for prostatic carcinoma had significant elevations of both leucocyte glycogen and LAP while similar patients not receiving estrogens had normal values.The results show that female sex hormones, specifically estrogens, can result in elevation of both leucocyte glycogen and LAP.
Fibrinolytic Activity and Haemagglutination Inhibition Immunoassays
Authors: Gormsen Johs , Vad Anne 
Published: August 2010

Concentrations of fibrinogen‐fibrin break‐down products (b.d.p.), above 10 üg/ml of serum (+EACA), estimated by haemagglutination inhibition immuno assays, were seldom found in healthy people. In 51 patients with coronary sclerosis and in 31 with other atherosclerotic diseases the amounts were in the lower part of normal range. The mean concentration in 16 patients with metastatic carcinoma was 86.7 and in 16 with hepatic cirrhosis 21.1 üg/ml. 93 patients with other diseases were examined. Huge amounts might be found even when fibrinolytic activity in the blood was very low. Several experiments indicate that b.d.p. also are formed extravascularly and presumably also by other proteases than plasmin.Short‐term stimulation of fibrinolytic activity with nicotinic acid (41 patients), venous stasis (35 pts.) and physical exertion (21 pts.), and long‐term stimulation with male hormones (5 pts.), metformin (4 pts.), clofibrate (3 pts.), tolazamid (4 pts.) and ethyloestranol + diguanides (11 pts.) was not followed by in vivo increase in b.d.p.
Formation of Granulocytes and Macrophages in Diffusion Chamber Cultures of Mouse Blood Leucocytes
Authors: Benestad Haakon Breien 
Published: August 2010

Suspensions of mononuclear cells were prepared from mouse blood by a one stage centrifugation method. Iso‐ and homologous culturing of these cells were then carried out in cell‐impermeable diffusion chambers. These were implanted into the peritoneal cavities of host mice. The culture periods ranged from 1 to 13 days. The proliferation, differentiation and death of the chamber cells were quantitated by total and differential cell counts and by evaluation of 3H‐thymidine incorporation from liquid scintillation counting and radioautography.Proliferating and differentiating granulocytes and macrophages were consistently found in the cultures, whilst lymphocytes from the inoculum were gradually lost during the culture periods.
Vitamin B12 and Its Binding Proteins in Cirrhosis and Infectious Hepatitis
Authors: Jørgensen F. Schønau 
Published: August 2010

The total B12‐binding capacity of serum and the unsaturated B12‐binding capacity of the transcobalamine TC I and TC II have been determined by gel filtration on Sephadex in 10 patients with infectious hepatitis and 9 with cirrhosis of the liver. For comparison 5 normal persons have been examined.The total B12‐binding capacity was equal in the 3 groups, the unsaturated B12‐binding capacity was markedly reduced in cirrhosis and hepatitis involving both TC I and TC II suggesting an increase in the endogenous binding of B12 to both TC I and TC II.
The Presence of Non‐Recirculating Long‐Lived Lymphocytes in Rat Blood
Authors: Iversen Jens‐Gustav , Benestad Haakon Breien 
Published: August 2010

An attempt has been made to find out if all the long‐lived lymphocytes in the rat are recirculating between blood and lymph.Selective labelling of long‐lived lymphocytes was obtained by daily injections of 3H‐thymidine for a period of 2–3 weeks, and by subsequently waiting at least 14 days after the last injection. The rats were then depleted of recirculating lymphocytes by continuous drainage of thoracic duct lymph for 2–4 days. The content of labelled cells in blood and thoracic duct lymph at various stages during the drainage period was evaluated by radioautographic and radiochemical techniques. The lymph depletion caused a reduction in the percentage of labelled cells in thoracic duct lymph, but not in blood, where actually an increase in the percentage of labelled lymphocytes was recorded.The results suggest that there is a group of long‐lived lymphocytes in rats which do not recirculate between blood and lymph.
On the Origin of Foà‐Kurloff Cells
Authors: Ernström Ulf , Sandberg Göran 
Published: August 2010

The thymic and splenic release of Foà‐Kurloff cells into the blood was studied in estradiol‐treated male guinea pigs by comparison between the cellular content in afferent and efferent blood. The amount and distribution of such cells in thymus, spleen, lymph nodes, and bone marrow was investigated.The treatment with estradiol caused involution of the thymus and splenomegaly. An abundance of Foà‐Kurloff cells was found in the red splenic pulp and a considerable release of such cells from the spleen into the blood was demonstrated. At the same time the output of lymphocytes from the spleen was reduced, suggesting that the Foà‐Kurloff cells are transformed lymphocytes. The spleen contained an increased amount of erythroblasts, indicating a stimulation of splenic erythropoiesis by estradiol.In the bone marrow and the thymus the number of Foà‐Kurloff cells was much smaller than in the spleen and no emigration of such cells from the thymus into the blood was demonstrated. A very small amount of Foà‐Kurloff cells was found in the lymph nodes and very few occurred in the thoracic duct lymph. Thus, the Foà‐Kurloff cells of the blood do not originate in the lymph nodes and do not recirculate between blood and lymph.It is concluded that the spleen is the major producer of Foà‐Kurloff cells and that they are released from the spleen into the blood.
Inhibition of the Function of Human Blood Platelets in vitro by VK 744
Authors: Sixma Jan J. , Trieschnigg Annemiek M. C. 
Published: August 2010

The effect of VK 744 (an analogue of dipyridamole) on the function of human blood platelets was studied. VK 744 inhibits collagen aggregation at a concentration of 25 μM. Preincubation at 37° C enhances the effect. ADP aggregation is inhibited at 50 μM. Adrenaline aggregation is likewise inhibited, and becomes reversible at 50 μM. VK 744 causes active disaggregation when added during ADP aggregation. The glass‐bead column retention and clot retraction are not inhibited until a concentration of 1 mM is attained. The glass adhesiveness according to Breddin is inhibited by 10 μM.The availability of platelet factor 3 as well as serotonin uptake are inhibited at the ED 50 of 6 μM and 0.2 mM, respectively. Comparison with RA 233 disclosed that two separate pharmacological receptors are involved. A study of serotonin release in collagen aggregation revealed that VK 744 is a primary inhibitor of aggregation. The log dose‐response relation during ADP aggregation with and without VK 744 suggests that this substance acts as a functional inhibitor.