bluuebunny/crossref_metadata_2025_split huggingface dataset:
Molecular Detection and Clinico-Therapeutic Studies of Contagious Ecthyma in Goat
Authors: Matre C.B. , Sakhare M.P. , Siddiqui M.F.M.F. , Shafi T.A. , Shaikh S.R. , Suman Pankaj , Sakhare Swati 
Published: May 2024

Abstract

Contagious ecthyma or Orf is a common, contagious and zoonotic disease of small ruminants caused by orf virus. The present research was conducted to study the prevalence of Orf in and around Parbhani and to evaluate efficacy of different treatment against Orf in goat. Overall prevalence of Orf in goat was recorded 38.83%. The higher prevalence of Orf recorded in the kids below 4 months of age, male, poor health status and non-descript goats. The overall clinical signs in contagious ecthyma goats were pyrexia, severe proliferative ulcerated cauliflower-like lesions around lips and scabby lesions were commences on the lips, muzzles, mucocutaneous junctions, nostrils, ear, inner aspect of thigh and gums. The hematological parameters revealed erythrocytopenia, reduced hemoglobin, leukocytosis, neutrophilia and lymphopenia. For molecular confirmation, DNA extraction of all scab samples collected from suspected goats were showed amplification of partial B2L gene at 592 bp by PCR. Analysis of DNA sequence revealed that three Parbhani sequences were positive for CEV. The obtained 3 sequences of orf virus got accession numbers PP235095, PP235096 and PP235097 were closely related with each other and with other strains obtained from GenBank for the Parbhani district, Maharashtra and strains of other states of India mainly with Meghalaya, Assam and Goa. The present study concludes that aciclovir @ 10 mg/kg along with neem leaves extract and curcumin powder locally was effective in treating the CE in goats early as compared to other two groups.
A Predictive Sequence Filling Method using Protein Structures for Early Detection of Alzheimer Disease
Authors: A.G Balamurugan , N Gomathi 
Published: May 2024

Abstract

Alzheimer’s disease (AD) is a generic form of dementia causing memory loss and environmental responses. AD detection is pursued using the different protein structures and their intensity based on different physical behaviors. Using the time-series protein structures the detection and is eased through the proposed neural method for structural protein filling (NC-SSF). Structural differentiations are performed using the high and low intensity profiles observed. This analysis identifies the missing inputs and thereby the fillable sequences are identified. The protein biomarker determines the maximum filling requirement as per the changes observed. The neural network is trained using this sequence required under the low and high intensity variations. This process is recurrent until maximum false rate is confined through accuracy improvements. The AD progression detection is performed by estimating the intensity under different profile filling levels. The proposed method improves accuracy, sensitivity, and specificity by 8.74%, 10.29%, and 8.84% respectively. This method reduced the false rate and MMSE by 9.85% and 10.78% respectively.
Use of new-media health information among the elderly and its effect on their health and health management
Authors: Xie Haojie , Wu Xudong , Zhou Yuqiu , Wang Mengyao , Xu Chunmiao , Li Min , Zhang Min , Wei Xuelian , Li Guiling 
Published: May 2024

Abstract

The aim of this study to explore the use of new-media health information and its influence on their health and health management.
Methods: A total of 208 elderly people in Qiqihar City were selected as the survey objects, and a questionnaire was used to investigate their access to health information through new-media platforms. We analyze the factors ( e.g., education、pre-retirement occupation,etc) in the use of health information on new-media platforms.
Results: Monthly income, educational level, pre-retirement occupation and attention of the new-media of the elderly had significant effects on health information acquisition (p<0.05).
Conclusion: The use of new-media health information among the elderly was diversified and had an impact on their health management. We should standardize the management of new-media health information dissemination platforms and publicize the use of new-media health information in a targeted way, so as to improve the self-health management of the elderly and reduce their burden on the national medical system.
Effective Strategies for Creating Self-Compatible B. rapa by Introgression of Mutated SRK and SCR/SP11 Genes from B. napus
Authors: Zhang Xueli , Heng Shuangping , Xiao Chunxiu , Liu Cong , Song Liping , Tang Liguang , He Congan , Wang Bincai , Wang Aihua , Gao Changbin 
Published: May 2024

Abstract

SI (Self-incompatibility) is one of the major obstructions for the development and propagation of inbred lines in most Brassica vegetable crops. The manipulation of SI genes is an effective way to create Self-compatible (SC) materials. A mutated pollen recognition specificity gene BnSP11-1 on the A genome was responsible for the self-compatibility of the B. napus (AACC) cultivar ‘Westar’. In this study, site-specific manipulation of stigma recognition specificity gene BnSRK-1 was carried out using the CRISPR/Cas9 system in ‘Westar’. Then the mutated SI genes (BnSP11-1 and BnSRK-1) were transferred to B.rapa by distant hybridization, by continuous back cross and self pollination, 3 elite and completely self-compatible lines were obtained. The mutated SI genes can be applied widely in Brassica vegetable crops with AA genome (2n=20), such as Chinese cabbage, Pak choi, Purple flowering stalks and Chinese flowering cabbage to accelerate the breeding process.
Physiological Responses and Accumulation of the Emerging Contaminant Ivermectin Using Salvinia Minima
Authors: Lacava Judith Elizabeth , Olivelli Melisa Soledad , Peluso Julieta , Aronzon Carolina Mariel , Quintana Rubén Darío 
Published: May 2024

Abstract

Ivermectin (IVM) is a macrocyclic lactone widely used to control endo- and ectoparasites in livestock. After administration, it is partially metabolized by the animal and therefore excreted in its original form. IVM can enter water bodies through groundwater, runoff, soil erosion, and direct deposition. Once in aquatic and wetland environments, due to its chemical characteristics, can persist for a long time, increasing its environmental risk. Macrophytes are in frequent contact with this drug, resulting in chronic exposure and leading to an accumulation process. The objective of this study is to evaluate the uptake of IVM in S. minima, and its phytotoxicity potential. Bioassays were performed to expose S. minima to different concentrations of IVM, 5 mg/L, 10 mg/L, and 30 mg/L. After 10 days, the accumulation of the compound in fronds, roots, and effluent was measured. Morphological parameters and photosynthetic pigments were evaluated. IVM was found in fronds and roots of S. minima after exposure. The percentage of remotion of the drug in effluent were significantly, up to 66%. The highest concentration evaluated showed phytotoxic effects. S. minima proved to be a promising species for IVM removal processes and early toxicity marker physiological parameters, especially in wetlands subject to intensive livestock farming activities. Of interest for its applicability in wetlands subjected to intensive livestock farming.
Indications and yield of Fresh Frozen Plasma in massive hemorrhage, efficiency biological monitoring. Preliminary results
Authors: Duranteau Olivier , Decamps Justine , Daper Anne , Cauchie Philippe , Ickx Brigitte , Tuna Turgay 
Published: May 2024

Abstract

The aim of the study was to examine various haemostasis values to identify the most relevant biological indicators for detecting significant hemorrhage, to determine the effectiveness of fresh frozen plasma (FFP) transfusion. Our findings suggest that a low prothrombin time, elevated Von Willebrand Antigen, increased plasma fibrinogen, and reduced Ca2+ levels are associated with challenges in achieving proper hemostasis. However, measurements of factors II, V, VII, VIII, IX, X, XI, XIII, protein C, and protein S do not appear to be linked to difficulties in achieving adequate haemostasis. Additionally, the administration of FFP appears to impact factors V, VII, X, and II.
Trial registration
EudraCT number: 2019-002898-64
Controller Reliability Assessment Model: A New Method for Reliability Assessment of Attitude Control Subsystem in Space Missions
Authors: Damircheli Mahrad , Fakoor Mahdi 
Published: May 2024

Abstract

The objective of this research is to assess the reliability of the controller and attitude control subsystem (ACS) of a LEO satellite using the presented Controller Reliability Assessment Model (CRAM). CRAM is based on the Software in the Loop (SIL) simulation which leads to the construction of a database from ACS performance. Using data analysis techniques leads to a comprehensive understanding of the ACS’s controller functionality and determines the failure rate of the controller’s component. In addition, Reliability Block Diagram (RBD) is implemented to visualize the contribution of the controller and estimate the reliability of the controllers according to the evaluated failure rates and reliability mathematical model for three years of operating. CRAM defines the relatively most robust, and accurate controller with the fastest response and highest reliability. In the designed control system, solar radiation, gravity gradient, and aerodynamic effects are considered as external disturbances. The actuator's loss of effectiveness is considered as an internal disturbance. The Tetrahedron configuration of reaction wheels is modeled in which three reaction wheels serve as three-axis control, and one reaction wheel as a redundant actuator. In this research, controllers such as PD, H∞, and two different types of sliding mode controllers are considered.
Research on Optimal Selection of Drainage Gas Recovery Technology Based on CRITIC-AHP Combination Weighting and Mahalanobis distance TOPSIS Method
Authors: Chenhao Guo 
Published: May 2024

Abstract

Drainage and gas production technology is an important process technology for increasing production in gas fields in the later stage. In order to optimize the drainage and gas production technology for gas wells with liquid accumulation, a TOPSIS model based optimization study was conducted based on the technical and economic adaptability analysis of different drainage and gas production technology and well condition parameters. This model characterizes the priority level of each alternative solution by calculating the distance and relative progress between each alternative solution and the ideal solution. In response to the problem that the weights determined by traditional single weighting methods cannot meet the principle of subjective and objective unity, the CRITIC-AHP combination weighting method is proposed. Based on the subjective and objective weighting, the optimal weight is calculated based on the concept of game theory; A Mahalanobis distance calculation model based on the modified Cosine correlation coefficient matrix and absolute ideal point representation is proposed to address the Euclidean distance failure and reverse order issues caused by the correlation and dimensional inconsistency between indicators in the TOPSIS method. Combined with actual production data, a comprehensive evaluation system for economic and technical indicators of drainage and gas production processes is established, and the well condition parameters of XX well are selected for optimal drainage and gas production processes. The results show that after implementing the drainage gas production process, the average water production of XX well increased from 29.41m3/d when liquid was accumulated, and the gas production was less than 1200m3/d. The gas production increased to 8500-9200m3/d, with a significant increase in production.
Mapping of Zika virus-host protein-protein interactions using yeast two-hybrid system and identification of re-purposed drug targets
Authors: Komarasamy Thamil Vaani , Adnan Nur Amelia Azreen , Balasubramaniam Vinod RMT 
Published: May 2024

Abstract

Zika virus (ZIKV) garnered global attention over the past decade as outbreaks of the disease were linked to neurological complications. There are currently no antiviral drugs or vaccines with proven efficacy. The identification of human proteins targeted by ZIKV is crucial for deciphering the host pathways hijacked by the virus to replicate, evade innate immunity, and induce neuropathogenesis. In our study, Y2H screening of 8 ZIKV proteins identified 85 interacting host factors. These host factors are primarily involved in immune and inflammatory responses, cell death, centrosome and cell cycle regulation, ubiquitin pathways, central nervous system (CNS) development and neurological disorders. Rho associated coiled-coil containing protein kinase 2 (ROCK2) was selected for further evaluation of its role in ZIKV infection. Immunofluorescence staining showed colocalization between ROCK2 and ZIKV NS5 in the cell nucleus, indicating the interaction likely contributes to viral replication via immune and inflammatory responses. Further siRNA knockdown of ROCK2 resulted in significant inhibition of ZIKV genome copy number. The ZIKV-host interactome was also used as a platform to identify druggable human proteins targeted by existing host-directed antiviral drugs. Taken together, findings of this study aid in improving the understanding of ZIKV pathogenesis and identifying potential therapeutic targets.
Design of Parallel Cascade Controller for Nonlinear Continuous Stirred Tank Reactor
Authors: Siddiqui Mohammad Atif 
Published: May 2024

Abstract

This work presents an approach to control the temperature of a nonlinear continuous stirred tank reactor (NCSTR) through parallel cascade control structure (PCCS). For the first time, PCCS is used to control the temperature of NCSTR by (1) modelling the dynamic behavior of CSTR with a recirculating jacket heat transfer system into a third order unstable transfer function and (2) using the model matching technique to synthesize the controller parameters. The controller of the secondary loop of PCCS is designed to achieve enhanced regulatory performance whereas, the primary loop controller is designed for better setpoint tracking. The closed loop performance of the proposed method is evaluated by carrying out simulation on the differential equation of the NCSTR and comparing it with other structures such as series cascade control structure (CCS) and parallel control structure (PCS). The response shows that the proposed method provides satisfactory performance in both the environment nominal, perturbed and noisy conditions.
RETRACTED: Genomic Footprints of Selection for Enhancing Productivity in Taiwanese Vegetable Soybeans through Core Collection Framework
Authors: Huang Yen-Hsiang , Chen Ling-Yu , Chen Shu-Yun , Kao Pei-Hsiu , Chen Shu , Kao Chung-Feng 
Published: May 2024

Abstract

The authors have requested that this preprint be removed from Research Square.
Edge and 2D states disentanglement and light-driven spin injection in MoS2
Authors: Garcia Flavio , Torrão Rodrigo , Safeer Syed , Marroquin John , Felix Jorlandio , Carozo Victor , Sampaio Luiz 
Published: May 2024

Abstract

Semiconductor transition metal dichalcogenides are a promising archetype for spintronic devices as they present several spin-to-charge interconversion mechanisms. Nevertheless, the microscopic origin of this interconversion is yet to be
established. Here, we report a comprehensive study of light-induced spin pumping in YIG/MoS2 heterostructures. In MoS2 monolayer microsized flakes, two distinct contributions to the spin current injection were identified. One from the metallic edge states and another from the 2D semiconductor states. A competitive interplay between the edge and 2D contributions was observed by changing the average flake size. We demonstrate that a light-driven spin current injection can enhance, attenuate, or even switch on/off the spin pumping depending on the size of the MoS2 flakes. The spin pumping in the MoS2 was independent of the film thickness, consistent with the inverse Rashba-Edelstein effect at the YIG/MoS2 interface. These findings highlight distinct contributions to spin pumping in transition metal dichalcogenides and open avenues for developing opto-spintronic device applications.
Transcriptomics analysis reveals the molecular mechanism of male sterility in Chimonanthus praecox
Authors: Liu Bin , Wu HuaFeng , Cao YinZhu , Yang XiMeng , Zhu Ting , Sui ShunZhao 
Published: May 2024

Abstract

Background Chimonanthus praecox is a unique ornamental and economic flowering tree in China, and after thousands of years of cultivation, it has produced a large number of varieties and a rich variety of types. C.praecox utilizes heterozygous and dioecious mating systems that are mainly heterozygous and partially autogamous, thus ensuring the inheritance of good genes of the species, and promoting the improvement of plant varieties. Male sterility is widespread in flowering plants and is an important tool for the genetic improvement of plants and optimization using hybrid advantage, but there has never been any report on male sterile material and related studies in C.praecox.
Result In this study, we reported for the first time the plant material of male sterility in C.praecox and preliminarily investigated the molecular mechanism of male sterility using transcriptome technology. The results showed that male sterility in C.praecox was already formed during the budding period and presumably occurred during the pre-mature stage of pollen cell maturation. It was also found that differentially expressed genes in starch and sugar metabolic pathways and phytohormone signaling pathways showed regular expression trends.
Conclusion This study reported for the first time the germplasm resources of male sterility in C.praecoxand explored the molecular mechanism of their male sterility, which can provide new ideas for the cultivation and genetic improvement of C.praecox as well as the study of the molecular mechanism of male sterility formation.
Situational appraisal of post-fire wildlife occupancy on a mixed-use ranch in Kenya
Authors: Mwangi Evans Mungai , Njueini John Kabue 
Published: May 2024

Abstract

Fire is integral to the ecology of tropical savannas. This study evaluated the scenario after a huge outbreak on part of a mixed wildlife-livestock ranch one year earlier. Driving along the roads and tracks to search for animals on three consecutive days, findings confirmed that the study area remained a wildlife stronghold, with a large proportion of large mammal species being spotted in the portion affected by the fire over the three-day survey. Overall, large animal distribution was significantly different between the unburnt and burnt area (t = 3.712569, df =36, P<0.001**). A similar pattern was only evident for the elephant (t= 2.819678, df =4, P<0.05*) but no other species. The disturbance was akin to ‘beneficial’ prescribed burning.
Bayesian Estimation of Missile Hit Accuracy for Dirichlet Distribution Based on Multiple Stages Growth Tests
Authors: Liu Haobang , Shi Xianming , Hu Tao , Chen Tong 
Published: May 2024

Abstract

During the period of research and design of new missiles, the hit accuracy tests are usually carried out in multiple stages according to the task progress and results. The existing estimation methods seldom consider the tests characteristics of multiple stages growth of missile hit accuracy, which bring difficulties to accurately estimate the missile hit accuracy. Considering the different degrees of damage caused by missile hitting the target in different areas, the Dirichlet distribution is selected as the prior distribution of missile hit accuracy parameters based on the Bayesian method. The sequence constraint relationship between the missile hit accuracy parameters of each stage test is established, and the Bayesian estimation model of missile hit accuracy based on the multiple stages growth tests is constructed. The Markov Chain-Monte Carlo (MCMC) method combined with Gibbs sampling are used to overcome the problem of solving the posterior high-dimensional integral of the model, and the finally estimation of missile hit accuracy is completed. The example shows that compared with the existing single stage test method, this method can consider the tests characteristics of multiple stages growth of missile hit accuracy and integrate the test information of each stage, which can provide reference for the estimation of missile hit accuracy during the period of research and development.
The characteristics of Laguerre higher-order cosh-Gaussian beam propagating in uniaxial crystal orthogonal to the optical axis
Authors: Benzehoua H. , Saad F. , Belafhal A. 
Published: May 2024

Abstract

In this study, we examine the propagation characteristics of Laguerre Higher Order Cosh-Gaussian beam (LHOchGB) in uniaxial crystals. Utilizing integral of Huygens-Fresnel, analytical expression of the LHOchGB in the considered crystal is derived. During beam propagation, our analyses are performed using numerical simulations under different parameters to investigate the impact of beam parameters and crystal refractive indices on the received beam. The obtained results demonstrate that the features of the incident beam and the crystal's refractive indices can be adjusted to control the intensity and phase distributions of the corresponding beam. We anticipate that the insights gleaned from our research will prove valuable for controlling optical particles.
Identification of a Novel Aerobic Promoter for efficient poly(3-hydroxybutyrate) Synthesis in Recombinant Escherichia coli
Authors: Liu Yu , Ma Xuelian , Wang Huan , Liu Dan , Yang Xi , Gao Qiang 
Published: May 2024

Abstract

A synthetic promoter library was developed to substitute the native promoter of phaCAB from Ralstonia eutropha in order to produce poly(3-hydroxybutyrate)(P3HB) in Escherichia coli. The library yielded over 141 successfully characterized clones, from which 35 unique promoters were identified by Sanger sequencing. The synthetic promoter P1 was shown to be particularly effective in driving the expression of downstream genes, including sfGFP and phCAB gene clusters. The performance of P1 exceeded that of the native promoter, achieving P3HB production levels of up to 79.78 ± 3.13% under aerobic conditions. Statistical analysis revealed that P1 significantly outperformed the native promoter of phCAB under aerobic conditions (P < 0.05), while displaying comparable activity under microaerobic conditions (P > 0.05).