bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bats (Mammalia: Chiroptera) from two priority areas for biodiversity conservation in the Brazilian Amazon and range extension for Carollia benkeithi (Phyllostomidae)
Authors: Soeiro Andreza , Bezerra Alexandra M. R. 
Published: May 2024

Abstract
Amazonian tropical forests host the highest bat diversity in South America. The central area Brazilian Amazon encompasses one of the largest pristine areas within this biome, where Amanã and Mamirauá Sustainable Development Reserves (SDRs) are located. These two reserves hold the status of Priority Areas for Biodiversity Conservation due to their high biological relevance. The aim of this study was to identify the bat species richness within these SDRs to minimize the Wallacean shortfall of the chiropterofauna in the Amazon. A total of 274 specimens, collected 20 years ago and housed in the mammal collection at the Museu Paraense Emílio Goeldi, were sorted and identified. They are distributed across four families, 18 genera, and 26 species: Artibeus planirostris and Carollia perspicillata were the most abundant vouchers. We identified a range extension for Carollia benkeithi and a possible new taxon for the genus Micronycteris.
Forage yield, seed, and forage qualitative traits evaluation by determining the optimal forage harvesting stage in dual-purpose cultivation in safflower varieties (Carthamus tinctorius L.)
Authors: Kargar Mohammad Hadi , Sayfzadeh Saeed , Jabari Hamid , Zakerin Hamid Reza , Golzardi Farid 
Published: May 2024

Abstract
Safflower (Carthamus tinctorius L.), adapted to arid and semiarid regions, is grown for seed and petal production, but the present study aimed to evaluate the possibility of simultaneous forage and seed production in the autumn and middle types of safflower varieties in dual-purpose cultivation in semiarid areas in the shortfall forage period. An experiment was done based on a randomized complete block design with nine treatments and three replications at the Seed and Plant Improvement Institute, Agriculture Research, Education and Extension Organization, Karaj, Iran. The treatments included the cultivation of Parnian, Goldasht, and Golmehr varieties with the purpose of seed harvesting as control treatments; the same varieties (Parnian, Goldasht, and Golmehr), once with the aim of forage harvesting at 50% stem elongation stage and seed harvesting; and again with the aim of forage harvesting at 50% branching stage and seed harvesting. The results showed that forage harvesting at the 50% branching stage significantly increased the dry and fresh forage yield compared to the 50% stem elongation stage. The highest fresh and dry forage yields (DFY) (42,229 and 11,266 kg/ha) were related to the Golmehr variety at the 50% branching stage. Forage harvesting at the stem elongation stage decreased the crude protein, protein content, and digestibility compared to the branching stage in three safflower varieties. Parnian variety had the maximum seed yield (2,226 kg/ha) without forage harvesting. The highest seed yield in the second year (2018–2019) of the experiment belonged to the Golmehr variety (1,310 kg/ha) to harvest forage at 50% stem elongation (rapid stem growth) and seed harvest treatment, which compared to the first year (2017–2018), showed an increase of 25%. Finally, the forage harvested amount of all three varieties studied was higher in the branching stage than in the stem elongation stage. These results implied that the Golmehr variety (11,266 kg/ha DFY and 520 kg/ha seed yield) is for dual-purpose cultivation, and the Parnian variety (2,226 kg/ha seed yield) is suitable for only seed production for the semiarid region of Karaj in Iran and similar areas in terms of climate.
Histopathological Overview of Kidney and Liver Female White Rat Administered (Ananas comosus var. microstachys L.) Extract as Herbal Plant for Dysmenorrhea
Authors: Fitmawati Fitmawati , Saputra Agus , Teruna Hilwan Yuda , Juliantari Erwina 
Published: May 2024

Abstract Dysmenorrhea is an important clinical as well as social problem affecting more than 50% of menstruating women. Nanas bongsai (Ananas comosus var. microstachys L.) is commonly used as a medical plant, which local people believe of Riau Province Indonesia, as medicine to reduce pain while the menstruation period (dysmenorrhea). This study was aim to find histopathologic changes in the kidney and liver after being treated with nanas bongsai extract in the female white rat. Design experimental of this research is complete randomized design with 5 treatments. Each treatment was composed of two control (zero control given with water, positive control given with mefenamic acid) and nanas bongsai extract with 3 different dosage serials. Histology preparations were made by paraffin method and Hematoxylin-Eosin staining. The results showed that given nanas bongsai extract with three serial dosages towards the kidney show a picnosis in the nucleus. However, this damage did not affect the glomerulus structure. While observation towards the liver shows some injury, namely hydropic degeneration, lipid degeneration, and necrosis. Both of these damaged less than 25%. As a result, this percentage did not affect the structure of the kidney and liver. The results of this study indicate that there is no damage to the kidneys and liver due to the use of nanas bongsai so it is safe to use in herbal medicine and can be developed as a dysmenorrhea drug.AbstrakDismenore merupakan masalah klinis dan sosial yang penting yang memengaruhi lebih dari 50% wanita menstruasi. Nanas bongsai (Ananas comosus var. microstachys L.) dipercayai oleh masyarakat Desa Muara lembu, Kabupaten Kuantan Singingi, Provinsi Riau sebagai obat untuk mengurangi nyeri saat haid (dismenore). Penelitian ini bertujuan untuk mengetahui perubahan histopatologi pada ginjal dan hati setelah diberi perlakuan ekstrak nanas bongsai pada tikus putih betina. Rancangan percobaan yang digunakan adalah rancangan acak lengkap dengan 5 perlakuan. Masing-masing perlakuan terdiri dari dua kontrol dan ekstrak nanas bongsai dengan 3 seri dosis yang berbeda. Preparat histologi dibuat dengan metode parafin dan pewarnaan Hematoxylin-Eosin. Hasil penelitian menunjukkan bahwa pemberian ekstrak nanas bongsai dengan dosis tiga seri terhadap ginjal menunjukkan picnosis pada nukleus. Namun, kerusakan ini tidak memengaruhi struktur glomerulus. Sedangkan pengamatan terhadap hati menunjukkan beberapa cedera, yaitu degenerasi hidropik, degenerasi lipid, dan nekrosis. Keduanya rusak kurang dari 25%. Akibatnya, persentase ini tidak memengaruhi struktur ginjal dan hati. Hasil penelitian ini menunjukkan bahwa tidak ada kerusakan pada ginjal dan hati akibat penggunaan Nanas bongsai sehingga aman digunakan dalam pengobatan herbal dan dapat dikembangkan sebagai obat dismenore.
Unveiling the emergence of SARS-CoV-2 JN.1 sub-variant: Insights from the first cases at Charles Nicolle Hospital, Tunisia
Authors: Hamzaoui Zaineb , Ferjani Sana , Kanzari Lamia , Ben Ali Roua , Charaa Latifa , Landolsi Ichrak , Medini Ines , Chammam Sarra , Abid Salma , Ferjani Asma , Kebaier Dhouha , Fakhfakh Ahmed , Bouslah Zoubeir , Ben Sassi Mouna , Trabelsi Sameh , Boutiba-Ben Boubaker Ilhem 
Published: May 2024

AbstractThe JN.1 sub-variant is a new variant of the SARS-CoV-2 Omicron strain, derived from the BA.2.86 sub-variant. It was first detected in late 2023 and has quickly spread to many countries, becoming the most prevalent variant in some regions. JN.1 exhibits a unique mutation (L455S) in the spike protein compared to the BA.2.86 lineage, which may affect its transmissibility and immune evasion capabilities. JN.1 has been designated as a “variant of interest” by the World Health Organization due to its rapidly increasing spread and is being closely monitored for its impact on the COVID-19 pandemic. This study describes the emergence of SARS-CoV-2 JN.1 sub-variant in Tunisia, and reports its mutation profiles.Nasopharyngeal samples collected over a four-month period (October 2023 to January 2024) were subjected to RNA extraction and real-time RT-PCR confirmation of SARS-CoV-2 infection. The whole-genome sequencing was performed by an iSeq 100 sequencer and COVIDSeq kit reagents (Illumina, USA).Mutation analysis, using the NextClade platform and GISAID database, revealed the presence of JN.1 in 15 out of 80 positive cases (18.75%) during the study period.The emergence of JN.1 highlights the ongoing evolution of SARS-CoV-2 and the need for continued surveillance and research to better understand the characteristics and impact of emerging variants.
Drug-Induced Liver Injury (DILI) related to Propylthiouracil (PTU) in hyperthyroidism with liver cirrhosis
Authors: Salsabila Arina , Kholili Ulfa , Novida Hermina 
Published: May 2024

Link of Video Abstract: https://youtu.be/_dm9Vyddxvg

Background: Hyperthyroidism is an excessive concentration of thyroid hormone in tissues caused by increased thyroid hormone synthesis, excessive thyroid hormone release, or an endogenous or exogenous extrathyroid source. There are 3 types of hyperthyroid therapy: antithyroid drugs (PTU, thiamazole, and carbimazole), radioactive ablation, and surgery. However, PTU is one of the drugs included in the list of medications that cause Drug-induced Liver Injury (DILI).
Case Presentation: A-36-years old male came to the IRD Dr. Sutomo General Hospital with a chief complaint of weakness for 1 month. The patient also complained of yellowing of the skin on the body, especially in the eyes, for 1 month, and the stomach was getting bigger and bloated before admission to the hospital. The patient has had a history of hyperthyroidism since 6 months ago and has taken PTU for 5 months. Exophthalmos eyes, icteric eyes, ascites, and splenomegaly were found on physical examination. The laboratory results showed hyperbilirubinemia, an increase in transaminase enzymes, and hypoalbumin. In the hospital, the patient was given an infusion of albumin, tyrosol, UDCA, spironolactone, and propranolol.
Conclusion: It has been reported that a man, 36 years old, has an underlying disease of liver cirrhosis. The patient has habitual risk factors for consuming alcohol. The patient had received PTU therapy for 1 month and came to the hospital suspected of DILI. Based on these cases, it is necessary to be vigilant about administering medications to patients with underlying diseases.
Ultrasound evaluation of post-cesarean uterine wound healing based on a figure of eight suturing technique
Authors: Sitepu Makmur , Rizaldi Muhammad Birza , Djafar Siddik  
Published: May 2024

Link of Video Abstract: https://youtu.be/15Du23GFCWc

Introduction: A niche (also called an isthmocele) is a defect or uterine scar that can be detected by ultrasound a few weeks or months after delivery by cesarean section. A niche is often associated with obstetric complications in subsequent pregnancies, including uterine dehiscence and/or rupture, placenta previa, and placenta accreta. Placenta accreta is characterized by abnormal attachment of the placenta to the uterine wall and is one of the leading causes of maternal morbidity and mortality in developing countries. Therefore, this study aims to evaluate the healing of surgical wounds with the figure of eight suturing techniques.
Methods: This study included 32 primigravida patients who underwent cesarean section at RSIA (Mother & Child Hospital) Rosiva from March 2006 to July 2006. On day 6 of the puerperium, the uterine sutures were examined with transvaginal ultrasound. Patients were subsequently allowed to return to their homes to receive outpatient treatment. On day 40, the patients were requested to return to the hospital for a follow-up ultrasound examination.
Results: On day 6, incomplete wound healing was found in 31 patients (96.9%) and complete wound healing in 1 patient (3.1%). On day 40, 31 patients (96.9%) experienced perfect or complete healing. Statistical analysis showed a significant difference in wound healing between day 6 and day 40 (p = 0.0001).
Conclusion: On day 40 after cesarean section, as many as 96.9% of patients showed perfect/complete wound healing (type I) without a niche. Therefore, these patients had a low risk of obstetric complications associated with uterine scarring in subsequent pregnancies.
The effectiveness of platelet rich fibrin (PRF) graft to expression of IL-1 and TNF-α in cornea post NaOH exposure: an experimental study
Authors: Airlangga Marisa Surya , Prastyani Reni , Nurwasis  , Plumeriastuti Hani , Utomo Budi 
Published: May 2024

Link of Video Abstract: https://youtu.be/Qw5E3Le-WJk

Background: Alkaline trauma of cornea often causes irreversible vision loss, even though it is quickly treated. The effectiveness of anti-inflammatory therapy as a main treatment is still limited clinically, as well as has dangerous side effects. Platelet-rich fibrin (PRF) is quite safe and effective in the treatment of descemetocele and also important in wound healing process. PRF membrane also provides a mechanical function as a framework for cell proliferation, differentiation and migration which is important for tissue regeneration. This study aimed to analyse the effectiveness of PRF grafts on expression of interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) in cornea post NaOH exposure.
Methods: An experimental study was conducted on Oryctolagus cuniculus which was exposed to 1 N NAOH for 30 seconds. The study subjects were 14 rabbits consisting of 7 PRF graft rabbits and 7 control rabbits. The rabbit's right eye was then irrigated, dripped with 1% levofloxacin 1 drop and a PRF graft was placed.
The effectiveness of PRF graft was analysed by percentage of expression of IL-1 and TNF-α in cornea post NaOH exposure
Results: No significant differences were found in expression of IL-1 (p>0.05) and expression of TNF-α (p>0.05) in cornea post NaOH exposure. There was a decrease in the expression of IL-1 between control and PRF graft groups. The combination of the mechanical and chemotactic functions of PRF membranes makes them suitable as an autologous biomaterial for reconstruction, repair, and maintenance of ocular surfaces.
Conclusion: PRF plays a significant role in wound healing and preventing excessive fibrosis, therefore it has the enormous potential to reduce the expression of IL-1 in corneal alkaline damage.
Risk factors of ventilator-associated pneumonia in intensive care patients at tertiary referral hospital
Authors: Dewi Ayu Permatasari Tribuana Tungga , Mertaniasih Ni Made , Semedi Bambang Pujo , Alimsardjono Lindawati , Endraswari Pepy Dwi 
Published: May 2024

Link of Video Abstract: https://youtu.be/YEQ3ICJPmxA

Background: Ventilator-associated pneumonia (VAP) occurs after endotracheal intubation and ventilator for more than 48 hours therefore it is an emerging threat to patient safety. The aim of this study is to determine the incidence and compare the risk factors of ventilator patients diagnosed with ventilator-associated pneumonia and patients with ventilators who are not diagnosed with ventilator-associated pneumonia.
Methods: This study used a retrospective observational with a case-control approach. Data was collected from January 2019 to December 2021. The risk factors to be analysed were comorbidities, antibiotic therapy, length of stay in the intensive care unit, duration of ventilator use, and history of surgery.
Results: There were 18 patients in the VAP and the control groups met the inclusion and exclusion criteria. The incidence of incidents in 2019 was 3.2 per 1000 ventilator days; in 2020, it was 1.2 per 1000 ventilator days; and in 2021, it was 1.2 per 1000 ventilator days. Comparison test results using Mann-Whitney showed a difference with a p<0.05. Patients with prolonged use of ventilators (p<0.05) and prolonged hospitalization (p<0.05) have a higher risk of developing ventilator-associated pneumonia. The average VAP occurs on the fourth day on a ventilator. Gram-negative bacteria, Pseudomonas aeruginosa, Acinetobacter baumanii, and Klebsiella pneumoniae cause most bacterial infections
Conclusion: There is a high risk of developing VAP in patients with prolonged use of ventilators and extended stays in the intensive care unit of the Tertiary Referral Hospital.
Inverted Papilloma in a 61-year-old Man: A Case Report
Authors: Santosa Agus , Permatasari Dian , Laksmi Putu Pradnyasanti , Devinta Made Ratih Santi , Saraswati Made Ratna 
Published: May 2024

Introduction: Inverted papilloma is a benign epithelial tumor of cavum nasi and paranasal sinuses that originates on the mucosal surface of the sinonasal tract. The etiology of this tumor remains unknown although chronic sinusitis, allergy, and viral infections are suspected. Although it is a benign tumor, it has a tendency toward malignancy. We aim to present the report as a case of sinonasal inverted papilloma with clinical manifestations, and radiological and histopathological features in a 61-year-old male patient.
Case Presentation: A 61-year-old man presented to the Department of Ear, Nose, and Throat (ENT), Bali Royal Hospital on 17 January 2023 with left nasal obstruction 5 years ago, and getting worse over 3 months. Due to the obstruction, he felt his sense of smell diminished. He also often sneezed had headaches and felt his left nose was bigger than his right. On examination, a grey-white mass with an irregular, slippery surface, and soft consistency were found. The mass did not bleed easily, was not painful when touched, and filled almost the entire cavum nasi sinistra. CT scan revealed rhinosinusitis of maxillary dextra et sinistra, ethmoidal dextra et sinistra, and frontalis sinistra with polyp shape and concha nasi sinistra protruding posteriorly. Functional Endoscopic Sinus Surgery (FESS) and extirpation under general anesthesia (GA) were planned. An excisional biopsy was sent for histopathological examination. Based on clinicohistopathologic, it suggests of final diagnosis of inverted papilloma.
Conclusion: Due to the signs and symptoms of inverted papilloma are not specific, a CT scan and biopsy are needed to confirm the diagnosis. To reduce the recurrence of these tumors, complete tumor removal with an endoscopic approach is recommended. Long-term follow-up is also necessary to prevent the recurrence
An anterolateral thigh free flap for reconstruction of extensive neck defect in diabetic patient: a case report
Authors: Santoso Rosalyn Devina , Hamid Agus Roy Rusly Hariantana , Sanjaya I Gusti Putu Hendra 
Published: May 2024

Introduction: Reconstruction of the head and neck has challenges due to its three-dimensional structure, tissue complexity, extent of the defect, and clinical scenarios. Advances in microsurgical techniques make free tissue transfer reconstruction a popular choice to address these issues. Anterolateral thigh perforator flap (ALT) is a workhorse flap for head and neck reconstruction due to its long and reliable pedicle, large volume, versatility in composition, and low donor morbidity. However, diabetes mellitus may contribute to developing postoperative complications, such as vascular occlusions and thrombosis, that endanger free flap survival and are associated with poor wound healing. This case report demonstrates the successful utilization of this approach in the closure of extensive neck defects in a diabetic patient.
Case Presentation: A 56-year-old male was referred with a 21 cm x 15 cm defect following an odontogenic submandibular abscess. The flaw was extended from the intrabuccal, neck, and one-third of the upper anterior thorax, involving the skin, subcutaneous tissue, and exposed mandibular bone. The patient had previously uncontrolled diabetes mellitus. Surgical debridement, antibiotics, insulin for glucose control, and wound bed preparation were performed. A free fasciocutaneous ALT flap was used for defect closure. The flap was raised template-based, sized 24 × 15 cm, with a pedicle from the descending branch of a lateral circumflex femoral vessel. End-to-end anastomosis of one artery and vein of the superior thyroid vessel was performed. The donor site was closed using a split-thickness skin graft. The surgery and recovery were uneventful. Flap refinement was carried out one month later. The patient underwent a three-month follow-up period, expressing satisfaction with the aesthetic and functional outcomes.
Conclusions: Anterolateral thigh perforator flap (ALT) is a workhorse flap for reconstruction of head and neck reconstruction due to its long and reliable pedicle, large tissue volume with various compositions, and low donor morbidity. The LD flap presents as an alternative for free tissue transfer. Regional pedicled flaps can be chosen where microsurgery is not available or suitable. However, vascular compromise and flap failure remain a challenge. Identifying and managing risk factors such as diabetes mellitus are needed to minimize significant complications.
Tinnitus characteristics and risk factors in the Bali region
Authors: Gde Ngurah Indraguna Pinatih  , Eka Putra Setiawan  , Ni Putu Oktaviani Rinika Pranitasari  , I Putu Santhi Dewantara  , Pinatih Ketut Tadeus Max Nurcahya , I Made Nudi Arthana  
Published: May 2024

Introduction: It has been recognized that suitable external sounds can reduce or even make tinnitus impossible to hear. The working principle of sound therapy will be used to create a new tool or approach that suits the conditions of Indonesian society. This study aimed to describe the characteristics of tinnitus and its associated risk factors.
Method: This was a cross-sectional study conducted in Denpasar City, Gianyar Regency, and Badung Regency for one year. Patients who visited the ENT doctors' practices in these areas were interviewed with prepared instruments. The patients were then subjected to clinical and audiometric examinations. The data will be analyzed descriptively to get an overview of tinnitus's sociodemographic and clinical characteristics.
Result: A total of 101 participants were included in this study. Most tinnitus patients were unilateral on the left side of the ear. The most common sound is ringing, up to 70.3% of 101 research subjects, followed by sound types such as pulse and roaring sound. Furthermore, the frequency and amplitude of tinnitus sounds are quite varied, but the majority is dominated by high frequencies (3000 - 8000Hz). Based on the ear affected, the largest number of tinnitus sufferers were found in the left ear (47.0%). In this study, 82 people (81.2%) had a history of frequently using earphones, while 19 other people never used earphones in everyday life. Among all participants, 95 people (94.1%) were not exposed to noise at work.
Conclusion: The clinical characteristics of the tinnitus sound type were mostly ringing sounds, followed by pulse-like sounds and buzzing sounds. The frequency and amplitude of tinnitus sounds quite varied. Stress factors and the use of earphones occur in most tinnitus sufferers.