bluuebunny/crossref_metadata_2025_split huggingface dataset:
C‐Type LECTIN receptor‐like kinase 1 and ACTIN DEPOLYMERIZING FACTOR 3 are key components of plasmodesmata callose modulation
Authors: Kumar Ritesh , Iswanto Arya B. B. , Kumar Dhinesh , Shuwei Wu , Oh Kyujin , Moon Jiyun , Son Geon H. , Oh Eun‐Seok , Vu Minh H. , Lee Jinsu , Lee Keun W. , Oh Man‐Ho , Kwon Chian , Chung Woo S. , Kim Jae‐Yean , Kim Sang H. 
Published: May 2024

AbstractPlasmodesmata (PDs) are intercellular organelles carrying multiple membranous nanochannels that allow the trafficking of cellular signalling molecules. The channel regulation of PDs occurs dynamically and is required in various developmental and physiological processes. It is well known that callose is a critical component in regulating PD permeability or symplasmic connectivity, but the understanding of the signalling pathways and mechanisms of its regulation is limited. Here, we used the reverse genetic approach to investigate the role of C‐type lectin receptor‐like kinase 1 (CLRLK1) in the aspect of PD callose‐modulated symplasmic continuity. Here, we found that loss‐of‐function mutations in CLRLK1 resulted in excessive PD callose deposits and reduced symplasmic continuity, resulting in an accelerated gravitropic response. The protein interactome study also found that CLRLK1 interacted with actin depolymerizing factor 3 (ADF3) in vitro and in plants. Moreover, mutations in ADF3 result in elevated PD callose deposits and faster gravitropic response. Our results indicate that CLRLK1 and ADF3 negatively regulate PD callose accumulation, contributing to fine‐tuning symplasmic opening apertures. Overall, our studies identified two key components involved in the deposits of PD callose and provided new insights into how symplasmic connectivity is maintained by the control of PD callose homoeostasis.
Swimming was associated with rhinitis and pollinosis in a duration‐dependent manner
Authors: Kawabata Ayu , Motoyama Yuie , Takeuchi Jiro , Kusunoki Takashi 
Published: May 2024

AbstractBackgroundPrevious studies have reported conflicting results regarding the effects of childhood swimming on respiratory allergic symptoms. We investigated the relationship between swimming and respiratory allergic symptoms in schoolchildren.MethodsA questionnaire regarding participation in sports club activities and respiratory allergic symptoms in schoolchildren was distributed to the parents of all 6853 public school students (aged 6–14 years) in Omihachiman City, Shiga, Japan. The relationships between participation in sports club activities and the prevalence of respiratory allergic symptoms were analyzed by multivariable logistic regression analyses.ResultsQuestionnaires were returned for 4991 schoolchildren (response rate: 72.8%). Logistic regression analysis revealed significant positive associations between swimming and rhinitis (42.9% vs. 38.9%; adjusted odds ratio, 1.26; 95% confidence interval, 1.10–1.44), and swimming and pollinosis (32.1% vs. 28.1%; adjusted odds ratio, 1.28; 95% confidence interval, 1.11–1.47). The duration of participation in swimming activities was also significantly positively associated with the prevalence of rhinitis and pollinosis. Those who had participated in swimming activities for 6 years or more showed significantly higher prevalences of rhinitis and pollinosis (46.3% and 36.4%, respectively) than those without swimming activities (38.9% and 28.1%, respectively) and those who had participated in swimming activities for 5 years or less (40.1% and 28.5%, respectively).ConclusionsSwimming was associated with the prevalence of rhinitis and pollinosis in schoolchildren, especially among those who had participated in swimming activities for 6 years or more. Preventive measures and early interventions for rhinitis and pollinosis should be recommended to these children.
First evaluation of Eyberg Child Behavior Inventory for foster children in Japan
Authors: Matano Miyuki , Katabira Hisayo , Sekine Tomoe , Kurane Koyuru , Wakabayashi Kei , Kono Yumi , Tajima Toshihiro , Iwai Kosuke , Osaka Hitoshi , Monden Yukifumi 
Published: May 2024

AbstractBackgroundBehavioral problems of foster children are an important issue for the maintenance of the foster care system, but they have not been adequately studied in Japan. We used the Eyberg Child Behavior Inventory (ECBI) to investigate behavioral problems among foster children and to examine associated factors.MethodsTwenty‐nine foster children and their foster parents and 479 non‐foster children and parents were recruited for the foster and control groups, respectively. Both groups underwent statistical comparative analyses using data from their ECBI assessments. The ECBI has two scales: the Intensity Scale quantifies the severity of child behavioral problems, and the Problem Scale captures the caregiver's perceived difficulties handling each behavior. We conducted a retrospective investigation of the background of the foster parent–child pairs to explore potential causal relationships with behavioral problems.ResultsThe mean intensity score for the foster group was significantly higher than that for the control group (p = 0.001). The mean problem scores for the foster group and the control group were not significantly different (p = 0.79). In the foster group, the retrospective investigation revealed two children with neurological or neurodevelopmental disorders, 17 with histories of abuse, and 10 with other issues.ConclusionIntensity scores showed severe behavioral problems among foster children, perhaps caused by neurological disorders, abuse, parental mental health, or economic hardship. Problem scores showed no significant differences between groups. It can therefore be posited that foster parents might exhibit a more lenient parenting style when dealing with children who have a history of abuse by their biological parents.
GpEF1A: a novel lysine methyltransferase gene from Gypsophila perfoliata L. involved in boron homeostasis
Authors: Akbudak M. A. , Cirik N. , Erdeger S. N. , Filiz E. , Dogu S. , Bor M. 
Published: May 2024

Abstract

Rapid accumulation of boron (B) leads to toxicity in plant tissues, and the narrow gap between deficiency and toxicity makes it difficult to adjust essential B levels in soil for plant productivity. Therefore, understanding different aspects of B tolerance is necessary to provide new and valid solutions to B toxicity. Gypsophila perfoliata stands out as a remarkable example of a B‐tolerant plant, with a natural propensity to thrive in environments such as B mines and soils enriched with high levels of B.

In this study, a yeast functional screening experiment was conducted using cDNA libraries from G. perfoliata leaf and root cells for B tolerance.

Ten colonies from the leaf library grew in 80 mm boric acid, while none emerged from the root library. Analysis of isolated cDNAs showed identical sequences and a unique motif related to B tolerance. The gene GpEF1A was identified in the tolerant yeast colonies, with predicted structural features suggesting its role, and RT‐qPCR indicating increased expression under B stress.

A regulatory role for EF1A lysine methylation was proposed in mammalian cells and fungi because of its dynamic and inducible nature under environmental constraints. This could also be relevant for plant cells, as the high similarity of the GpEF1A gene in some salt‐tolerant plants might indicate the upregulation of EF1A as a conserved way to cope with abiotic stress conditions. This report represents the first instance of involvement of GpEF1A in B tolerance, and further detailed studies are necessary to understand other components of this tolerance mechanism.
Effects of demographic history on recombination hotspots in soybean
Authors: McConaughy Samantha , Amundsen Keenan , Hyten David 
Published: May 2024

SUMMARYRecombination is the primary mechanism underlying genetic improvement in populations and allows plant breeders to create new allelic combinations for agronomic improvement. Soybean [Glycine max (L.) Merr.] has gone through multiple genetic bottlenecks that have significantly affected its genetic diversity, linkage disequilibrium, and altered allele frequencies. To investigate the impact of genetic bottlenecks on recombination hotspots in soybeans, historical recombination was studied in three soybean populations. The populations were wild soybean [Glycine soja (Sieb. and Zucc.)], landraces, and North American elite soybean cultivars that have been genotyped with the SoySNP50K BeadChip. While each population after a genetic bottleneck had an increased average haplotype block size, they did not have a significant difference in the number of hotspots between each population. Instead, the increase in observed haplotype block size is likely due to an elimination of individuals that contained historical recombination at hotspots which decreased the observed rate of recombination for the hotspot after each genetic bottleneck. Conversely, heterochromatic DNA which has an increased haplotype block size compared to euchromatic DNA had a significantly different number of hotspots but not a significant difference in the average hotspot recombination rate. Previously identified genomic motifs associated with hotspots were also associated with hotspots found in the historical populations suggesting a common mechanism. This characterization of historical recombination hotspots in soybeans provides further insights into the effect genetic bottlenecks and selection have on recombination hotspots.
Transfusion‐associated acute lung injury following albumin treatment in liver disease
Authors: Hennawi Hussam Al , Okoyeze Kimberley , Boigon Margot , Nair Sunil 
Published: May 2024

AbstractBackground and ObjectivesTransfusion‐related acute lung injury is an infrequent adverse reaction observed in patients receiving blood products. The lung injury can range in severity and can be associated with both mortality and mortality. All blood products except albumin have been linked to cases of transfusion‐related acute lung injury. In fact, albumin may be used as a salvage modality in severe transfusion‐related acute lung injury. We report an alcoholic patient who developed lung injury following treatment with albumin in the setting of hypoalbuminaemia.Materials and MethodsA 41‐year‐old male with alcoholic liver disease was admitted for severe ascites and alcoholic hepatitis. Chest x‐ray showed small pleural effusions at the lung bases with no overt pulmonary oedema. He received high doses of furosemide for lower extremity oedema. The patient received a total of two albumin infusions to augment the diuresis effect.ResultsHe subsequently developed acute hypoxic respiratory failure with imaging showing interstitial and airspace abnormalities concerning for pulmonary oedema. He showed no additional signs of volume overload and was treated supportively until the condition improved.ConclusionThis is the first reported case of albumin‐associated lung injury proximally related to albumin infusion. We aim to increase awareness of this possible sequelae among physicians.
Quantitative upper bounds related to an isogeny criterion for elliptic curves
Authors: Cojocaru Alina Carmen , Hinz Auden , Wang Tian 
Published: May 2024

AbstractFor and elliptic curves defined over a number field , without complex multiplication, we consider the function counting nonzero prime ideals of the ring of integers of , of good reduction for and , of norm at most , and for which the Frobenius fields and are equal. Motivated by an isogeny criterion of Kulkarni, Patankar, and Rajan, which states that and are not potentially isogenous if and only if , we investigate the growth in of . We prove that if and are not potentially isogenous, then there exist positive constants , , and such that the following bounds hold: (i) ; (ii) under the Generalized Riemann Hypothesis for Dedekind zeta functions (GRH); (iii) under GRH, Artin's Holomorphy Conjecture for the Artin ‐functions of number field extensions, and a Pair Correlation Conjecture for the zeros of the Artin ‐functions of number field extensions.
Rational cross‐sections, bounded generation, and orders on groups
Authors: Bodart Corentin 
Published: May 2024

AbstractWe provide new examples of groups without rational cross‐sections (also called regular normal forms), using connections with bounded generation and rational orders on groups. Our examples contain a finitely presented HNN‐extension of the first Grigorchuk group. This last group is the first example of finitely presented group with solvable word problem and without rational cross‐sections. It is also not autostackable, and has no left‐regular complete rewriting system.
In vitro
and
in vivo
efficacy of the amiodarone and praziquantel combination against the blood fluke
Schistosoma mansoni
Authors: Cajas Rayssa A. , Santos Silvia S. B. , Espírito-Santo Maria Cristina C. , Garedaghi Yagoob , de Moraes Josué 
Published: May 2024

ABSTRACT

Schistosomiasis, a widespread parasitic disease caused by the blood fluke of the genus
Schistosoma
, affects over 230 million people, primarily in developing countries. Praziquantel, the sole drug currently approved for schistosomiasis treatment, demonstrates effectiveness against patent infections. A recent study highlighted the antiparasitic properties of amiodarone, an anti-arrhythmic drug, exhibiting higher efficacy than praziquantel against prepatent infections. This study assessed the efficacy of amiodarone and praziquantel, both individually and in combination, against
Schistosoma mansoni
through comprehensive
in vitro
and
in vivo
experiments.
In vitro
experiments demonstrated synergistic activity (fractional inhibitory concentration index ≤0.5) for combinations of amiodarone with praziquantel. In a murine model of schistosomiasis featuring prepatent infections, treatments involving amiodarone (200 or 400 mg/kg) followed by praziquantel (200 or 400 mg/kg) yielded a substantial reduction in worm burden (60%–70%). Given the low efficacy of praziquantel in prepatent infections, combinations of amiodarone with praziquantel may offer clinical utility in the treatment of schistosomiasis.
Characteristics of upper respiratory tract rhinovirus in children with allergic rhinitis and its role in disease severity
Authors: Gao Yingqin , Zhou Jienan , Wang Meilan , Liang Yanqi , Zhang Ting , Mao Yunxiang , Ma Jing , Li Li , Zhang Tiesong , Guo Lei 
Published: May 2024

ABSTRACT

Allergic rhinitis (AR) is a global health challenge that particularly affects the quality of life of children. Human rhinovirus (HRV) infection usually causes common cold in the upper respiratory tract (URT) and can also affect airway allergy development, such as asthma exacerbation, but its relationship with AR is poorly understood. The study aimed to gain insight into the characteristics of HRV that is prevalent in AR children and its role in AR severity. A total of 362 children with symptomatic AR were enrolled from southwestern China during 2022–2023, and nasal lavage samples were collected for HRV molecular characterization and cytokine measurement. HRV was detected in 40% of the AR children, with peak detection in autumn. The positive rate was not correlated with whether the subjects were under allergen-specific immunotherapy (AIT). Among the detected HRVs, 42% were species A, 36% were species B, and 22% were species C, involving 21 A genotypes, 6 B genotypes, and 7 C genotypes. HRV positivity was significantly associated with symptom severity (visual analog scale [VAS] score) and elevated levels of local nasal IgE, interleukin-25 (IL-25), IL-4, and CXCL13 in AR children who did not receive antiallergic treatment. All three species of HRV strains (A1B, A21, B27, B70, and C17) had been isolated and were able to infect respiratory epithelial tissue
in vitro
. Complete genome sequencing showed that the antigenic epitopes of the isolated HRVs had certain variations. Our work reveals the etiological characteristics of URT-HRV in AR children and suggests a role of HRV infection in the pathogenesis of childhood AR.

IMPORTANCE
Our study revealed high human rhinovirus (HRV) detection rate in children with allergic rhinitis (AR), and HRV infection (A, B, or C species) is positively associated with the symptom severity in AR children. Elevated nasal IgE, interleukin-25 (IL-25), IL-4, and CXCL13 levels suggest a potential pathogenic mechanism by which HRV infection induces nasal type 2 immune/inflammation responses and local IgE production in AR patients. In addition, etiological analysis found that the main prevalent HRV species in AR children are A and B (~80%), which is different from acute respiratory infection and asthma exacerbation, where species A and C are dominant. The data reveal the distinct species prevalence characteristics of HRV infection in AR. Finally, we isolated all three species of HRV strains from nasal cavity of AR children with varying degrees of antigenic epitope mutations and in vitro infectivity, highlighting the importance of strengthening monitoring and intervention for respiratory HRV infection in AR children.
Insights into the Phanerozoic evolution of the São Francisco Craton based on detrital zircon thermochronology and U-Pb-Hf geochronology
Authors: Dias Airton N.C. , Martins-Ferreira Marco Antonio C. , Pereira Vinicius Q. , Sales Antonio S.W. , Chemale Farid 
Published: May 2024

Abstract
Basins within cratonic environments offer valuable insights into the fragmentation, dispersion, and agglutination of the lithosphere within supercontinents. The southern São Francisco Craton is home to sedimentary basins that formed in both intraplate tectonic settings (late Paleoproterozoic to Neoproterozoic and Mesozoic) and convergent tectonic settings (Ediacaran to Cambrian). To elucidate the Phanerozoic thermal history of the craton and the long-term depositional history of the southern São Francisco Craton’s cover, a comprehensive study was conducted utilizing detrital zircons through zircon fission-track (ZFT) thermochronology and U-Pb-Hf geochronology. This study analyzed samples from the following units: (1) the intracratonic basin—Lower and Upper Espinhaço Supergroup, with depositional age intervals ranging 1800–1600 Ma and 1200–920 Ma, respectively; (2) the rift-related passive margin basin—Lower Macaúbas Group (720 Ma and 640 Ma) and Jequitaí Formation, which is likely of Cryogenian age (650–635 Ma); (3) and the foreland basin—Bambui Group and Três Marias Formation, with ages of ca. 600–520 Ma and 520–480 Ma, respectively. U-Pb-Hf detrital zircon geochronology revealed the complex depositional history of the southern São Francisco Craton, and indicated changing source rocks over time. Conversely, the ZFT analysis of Precambrian to Cambrian samples allowed for the identification and characterization of Phanerozoic events in the southern São Francisco Craton. These events include: (1) the exhumation and late orogenic uplift of the foreland at ca. 500–480 Ma (mean age of 499 ± 5.5 Ma) during the late stages of the Brasiliano–Pan-African Cycle, which was associated with the Araçuaí Belt and lithospheric rebound of the peripheral cratonic margin of the southern São Francisco Craton; and (2) an age of 330–280 Ma (mean age of 306.6 ± 4.8 Ma) that possibly resulted from ice retreat and isostatic rebound interior of the Gondwana Paleocontinent. Additionally, analysis of detrital zircons from the Areado Group shows a later tectonic event recorded at 128.1 ± 9.4 Ma, which indicates Mesozoic intracontinental rifting. Based on these results and interpretations, the timing and exhumation of the southern São Francisco Craton during the Phanerozoic can be attributed to the final stages of the continental collision of the southern São Francisco Craton and Congo Craton and large-scale continental uplift during the Permo-Carboniferous late Paleozoic Ice Age of Gondwana. Overall, the findings demonstrate a direct correlation between known tectonic events at the plate margins of Western Gondwana and periods of cratonic basin formation.
Late Oligocene–Miocene evolution of deep-water circulation in the abyssal South China Sea: Insights from Nd isotopes of fossil fish teeth
Authors: Shu Wei , Colin Christophe , Liu Zhifei , Dapoigny Arnaud 
Published: May 2024

Abstract
A long-term neodymium isotope (εNd) record of fossil fish teeth was investigated to constrain the evolution of deep-water circulation in the abyssal South China Sea (SCS) during the late Oligocene–Miocene (27–10 Ma). Fish teeth samples were collected from the oceanic red beds at International Ocean Discovery Program Expedition 367 Site U1499 (water depth 3758 m). Seawater εNd values (from –7.1 to –4.8, average –6.1) prior to 15 Ma indicate that water masses in the abyssal SCS resulted from the mixing of more radiogenic Upper Circumpolar Deep Water (UCDW, average –4.5) and less radiogenic Lower Circumpolar Deep Water (LCDW, average –6.4). The general decrease in εNd values was attributed to an increasing influence of the unradiogenic LCDW at the studied site, consistent with the subsidence and the associated deepening of the SCS plain. After 15 Ma, seawater εNd dropped significantly to a range of –8.9 to –6.1 (average –7.5), indicating a slowdown in the hydrological connection between the deep-water masses in the SCS and the western Pacific Ocean. We argue that the formation of the Luzon Strait due to the uplift of the Luzon arc in the late Miocene led to the shallowing and narrowing of the SCS-Pacific channels. Consequently, penetration of LCDW was reduced and water masses in the abyssal SCS would have been less ventilated and strongly influenced by lithogenic input from the unradiogenic sediments of large Asian rivers draining the peri-Himalayan region.
Fingerprinting enhanced floodplain reworking during the Paleocene–Eocene Thermal Maximum in the Southern Pyrenees (Spain): Implications for channel dynamics and carbon burial
Authors: Prieur Marine , Whittaker Alexander C. , Nuriel Perach , Jaimes-Gutierrez Rocío , Garzanti Eduardo , Roigé Marta , Sømme Tor O. , Schlunegger Fritz , Castelltort Sébastien 
Published: May 2024

Abstract
The sedimentary record of the Paleocene–Eocene Thermal Maximum (PETM, ca. 56 Ma) allows the study of feedback mechanisms over the entire duration of a climatic event, from carbon release to the subsequent recovery phase. Clay sedimentation increase in the oceans during the PETM is linked to enhanced terrestrial erosion. Fluvial channel mobility has been invoked to explain this increase in fine sediment export based on more frequent transitional avulsions. In this study, we test whether the reworking of Microcodium (prismatic calcite concretions) from the floodplain to marine environments can serve to fingerprint floodplain reworking due to channel mobility. We quantified the abundance of floodplain-sourced Microcodium grains reworked in fluvial to marine sandstones pre-dating and coeval to the PETM in the Southern Pyrenees (Tremp Basin, Spain). Laser ablation–inductively coupled plasma–mass spectrometry U-Pb ages on calcite confirm the Thanetian age of the Microcodium grains. Our data show a four-fold increase in the export of floodplain sediments to the marine domain during the PETM. Moreover, we show that this is predominantly due to enhanced channel mobility, reworking channel banks and interfluves, with increased erosion in the hinterland as a secondary factor. This increase in floodplain reworking would correspond to an increase in biospheric carbon burial flux by a factor of 2.2. Therefore, enhanced channel mobility and fine-grain sediment transport to the oceans during a climatic perturbation such as the PETM may constitute an important negative feedback mechanism.