bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of systemic parathyroid hormone (1–34) and a β‐tricalcium phosphate biomaterial on local bone formation in a critical‐size rat calvarial defect model
Authors: Yun Jonathan I. , Wikesjö Ulf ME , Borke James L. , Bisch Frederick C. , Lewis Jill E. , Herold Robert W. , Swiec Gary D. , Wood Joseph C. , McPherson James C. 
Published: March 2010

Yun JI, Wikesjö UME, Borke JL, Bisch FC, Lewis JE, Herold RW, Swiec GD, Wood JC, McPherson III JC. Effect of systemic parathyroid hormone (1–34) and a β‐tricalcium phosphate biomaterial on local bone formation in a critical‐size rat calvarial defect model. J Clin Periodontol 2010; 37: 419–426 doi: 10.1111/j.1600‐051X.2010.01547.x AbstractObjective: The objective of this study was to evaluate local bone formation following systemic administration of parathyroid hormone (1–34) (PTH), a surgically implanted synthetic β‐tricalcium phosphate (β‐TCP) bone biomaterial serving as a matrix to support new bone formation.Materials and Methods: Critical‐size, 8 mm, calvarial through‐and‐through osteotomy defects were surgically created in 100 adult male Sprague–Dawley rats. The animals were randomized into five groups of 20 animals each to receive one of the following treatments: PTH (15 μg PTH/kg/day; subcutaneously), PTH/β‐TCP, β‐TCP, or particulate human demineralized freeze‐dried bone (DFDB), and sham‐surgery controls. Ten animals/group were euthanized at 4 and 8 weeks post‐surgery for radiographic and histometric analysis.Results: The histometric analysis showed that systemic PTH significantly enhanced local bone formation, bone fill averaging (±SE) 32.2±4.0% compared with PTH/β‐TCP (15.7±2.4%), β‐TCP (12.5±2.3%), DFDB (14.5±2.3%), and sham‐surgery control (10.0±1.5%) at 4 weeks (p<0.014). Systemic PTH showed significantly enhanced bone formation (41.5±4.0%) compared with PTH/β‐TCP (22.4±3.0%), β‐TCP (21.3±4.4%), and with the sham‐surgery control (23.8±4.2%) at 8 weeks (p<0.025). The DFDB group showed significantly increased bone formation from 4 (14.5±2.3%) to 8 weeks (32.0±3.2%) (p<0.006). The PTH/β‐TCP and β‐TCP groups both showed limited biomaterials resorption. The radiographic analysis was not diagnostic to distinguish local bone formation from the radiopaque β‐TCP biomaterial.Conclusions: Systemic administration of PTH significantly stimulates local bone formation. Bone formation was significantly limited by the β‐TCP biomaterial.
Ovarian pathology in rhesus macaques: a 12‐year retrospective
Authors: Marr‐Belvin A.K. , Bailey C.C. , Knight H.L. , Klumpp S.A. , Westmoreland S.V. , Miller A.D. 
Published: March 2010

AbstractBackground  Ovarian pathology is an important cause of decreased fertility and reproductive capability and may impact multiple systems, particularly in aging rhesus macaques.Methods  Retrospective histopathologic and immunohistochemical analysis of 458 female rhesus macaque necropsies over 12 years at the New England Primate Research Center in Southborough, MA.Results  Degenerative and inflammatory changes in the ovaries included mineralization, infiltration by lymphocytes, macrophages and multinucleated giant cells, endometriosis, and arteriopathy. Cystic changes included follicular cysts, cystic rete, and mesonephric duct cysts with cystic rete the most common. Neoplasms included granulosa cell tumors, cystadenoma, cystadenocarcinoma, and teratoma.Conclusions  Ovarian lesions of the rhesus macaque are similar to those of cynomolgus macaques and humans. These lesions are frequently incidental findings but may impact metabolic and neurocognitive studies.
Evaluation of a rapamycin‐regulated serotype 2 adeno‐associated viral vector in macaque parotid glands
Authors: Zheng C , Voutetakis A , Metzger M , Afione S , Cotrim AP , Eckhaus MA , Rivera VM , Clackson T , Chiorini JA , Donahue RE , Dunbar CE , Baum BJ 
Published: March 2010

Oral Diseases (2010) 16, 269–277Objectives:  Salivary glands are useful target organs for local and systemic gene therapeutics. For such applications, the regulation of transgene expression is important. Previous studies by us in murine submandibular glands showed that a rapamycin transcriptional regulation system in a single serotype 2, adeno‐associated viral (AAV2) vector was effective for this purpose. This study evaluated if such a vector was similarly useful in rhesus macaque parotid glands.Methods:  A recombinant AAV2 vector (AAV‐TF‐RhEpo‐2.3w), encoding rhesus erythropoietin (RhEpo) and a rapamycin‐inducible promoter, was constructed. The vector was administered to macaques at either of two doses [1.5 × 1011 (low dose) or 1.5 × 1012 (high dose) vector genomes] via cannulation of Stensen’s duct. Animals were followed up for 12–14 weeks and treated at intervals with rapamycin (0.1 or 0.5 mg kg−1) to induce gene expression. Serum chemistry, hematology, and RhEpo levels were measured at interval.Results:  AAV‐TF‐RhEpo‐2.3w administration led to low levels of rapamycin‐inducible RhEpo expression in the serum of most macaques. In five animals, no significant changes were seen in serum chemistry and hematology values over the study. One macaque, however, developed pneumonia, became anemic and subsequently required euthanasia. After the onset of anemia, a single administration of rapamycin led to significant RhEpo production in this animal.Conclusion:  Administration of AAV‐TF‐RhEpo‐2.3w to macaque parotid glands was generally safe, but led only to low levels of serum RhEpo in healthy animals following rapamycin treatment.
Neuroanatomical Evidence for a Role of Central Melanocortin-4 Receptors and Oxytocin in the Efferent Control of the Rodent Clitoris and Vagina
Authors: Gelez Helene , Poirier Sarah , Facchinetti Patricia , Allers Kelly A. , Wayman Chris , Alexandre Laurent , Giuliano François 
Published: March 2010

ABSTRACTIntroductionThe clitoris and the vagina are the main peripheral anatomical structures involved in physiological changes related to sexual arousal and orgasm. Their efferent control and, more particularly, the neurochemical phenotype of these descending neuronal pathways remain largely uncharacterized.AimTo examine if brain neurons involved in the efferent control of the clitoris and the vagina possess melanocortin-4 receptor (MC4-R) and/or contain oxytocin (OT).MethodsNeurons involved in the efferent control of the vagina and clitoris were identified following visualization of pseudorabies virus (PRV) retrograde tracing. PRV was injected into the vagina and clitoris in adult rats in estrous. On the fifth day postinjection, animals were humanely sacrificed, and brains were removed and sectioned, and processed for PRV visualization. The neurochemical phenotype of PRV-positive neurons was identified using double or triple immunocytochemical labeling against PRV, MC4-R, and OT. Double and triple labeling were quantified using confocal laser scanning microscopy.Main Outcome MeasureNeuroanatomical brain distribution, number and percentage of double-labeled PRV/MC4-R and PRV-/OT-positive neurons, and triple PRV-/MC4-R-/OT-labeled neurons.ResultsThe majority of PRV immunopositive neurons which also expressed immunoreactivity for MC4-R were located in the paraventricular and arcuate nuclei of the hypothalamus. The majority of PRV positive neurons which were immunoreactive (IR) for OT were located in the paraventricular nucleus (PVN), medial preoptic area (MPOA), and lateral hypothalamus. PRV positive neurons were more likely to be IR for MC4-R than for OT. Scattered triple-labeled PRV/MC4-R/OT neurons were detected in the MPOA and the PVN.ConclusionThese data strongly suggest that MC4-R and, to a less extent, OT are involved in the efferent neuronal control of the clitoris and vagina, and consequently facilitate our understanding of how the melanocortinergic pathway regulates female sexual function.
Effect of Sexual Function on Health-Related Quality of Life Mediated by Depressive Symptoms in Cardiac Rehabilitation. Findings of the SPARK Project in 493 Patients
Authors: Kriston Levente , Günzler Cindy , Agyemang Anja , Bengel Jürgen , Berner Michael M. 
Published: March 2010

ABSTRACT

Introduction
Empirical evidence suggests associations between cardiovascular diseases, sexual functioning, depressive symptoms, and quality of life. However, to date, the interrelation of these constructs has not been examined simultaneously in a structural analysis.

Aim
To estimate the prevalence of sexual disorders and depressive symptoms and to examine the association between sexual disorders, depressive symptoms, and quality of life in patients in the rehabilitation of cardiovascular disorders.

Aim
A postal survey in five German inpatient rehabilitation centers for cardiovascular diseases was conducted. Prevalence of sexual disorders and depressive symptoms were assessed using psychometrically sound instruments. To analyze complex associations, structural equation modeling was used.

Main Outcome Measures
For epidemiological questions, proportions with 95% confidence intervals were calculated. The strength of association in structural equation models was expressed as a standardized regression coefficient.

Results
Data from 493 patients were analyzed (response rate 22.7%). At least moderate erectile dysfunction proved to be present in 20.3% of men. The prevalence of female sexual dysfunction lay at 43.1%. At least moderate depressive symptoms were present in 14.4% of men and 16.5% of women. A considerable association between sexual functioning and quality of life was found in both sexes, which was largely mediated by depressive symptoms. Major drawbacks of the study are imprecision of the estimates due to limited sample size and questionable generalizability of the findings due to possible self-selection bias.

Conclusions
Considering the high prevalence of depressive symptoms and their role as a mediating factor between sexual functioning and quality of life, it is recommended to routinely screen for depression in men and women with cardiac disease.
The Efficacy and Safety of Testosterone Undecanoate (Nebido®) in Testosterone Deficiency Syndrome in Korean: A Multicenter Prospective Study
Authors: Moon Du Geon , Park Min Gu , Lee Sung Won , Park Kwangsung , Park Jong Kwan , Kim Sae Woong , Park Nam Cheol , Ahn Tai Young , Paick Jae Seung , Seo Ju Tae , Yang Dae Yul , Lee June Young , Kim Je Jong 
Published: March 2010

ABSTRACT

Introduction
Long-acting injectable testosterone undecanoate (TU, Nebido®), a new parenteral testosterone preparation, has recently been introduced to avoid frequent injections of the conventional injectable esters.

Aims
To assess the efficacy and safety of long-acting injectable testosterone undecanoate (TU, Nebido®) in Korean patients with testosterone deficiency syndrome (TDS).

Methods
One hundred thirty-three patients who complain of erectile dysfunction with serum testosterone level less than 3.5 ng/mL were injected with 1,000 mg of TU (4 mL/ample) on day 1, followed by another injection after 6 weeks and 18 weeks. For the safety profiles, serum hemoglobin (Hb), hematocrit (Hct), glucose, lipid profile, and prostate-specific antigen (PSA) were measured.

Main Outcome Measures
Body mass index (BMI) was measured at the time of the first visit and after 12, 24 weeks. Primary efficacy was evaluated according to changes in the International Index of Erectile Function (IIEF) from the initial visit to the final visit (24 weeks) and from the initial visit to each visit. Secondary efficacy was assessed with changes of the Aging Males’ Symptoms (AMS) Scale and the Global Efficacy Question (GEQ) for improvement of erectile function.

Results
Mean age of patients was 54 ± 9.6 years. Compared with pretreatment, no significant improvement in BMI was observed. Serum total testosterone and free testosterone were significantly increased at 12 weeks and were maintained until 24 weeks (P &lt;0.001). TU significantly decreased cholesterol (P &lt;0.0001). TU significantly improved total IIEF, all five domain scores of IIEF (P &lt;0.0001) and total AMS, all three domain scores of AMS (P &lt;0.0001). On GEQ, TU improved erectile function in 76.9% of subjects. On safety profile, TU significantly elevated Hb, Hct, and PSA at 24 weeks but within normal range. No serious adverse reactions were observed. Drop-out rate was 15.0%.

Conclusions
In this prospective multicenter study, TU was effective, safe, and tolerable until 24 weeks in Korean TDS patients. Further well-controlled, long-term study should follow.
HLA‐B*15 subtypes distribution in Han population in Beijing, China, as compared with those of other populations
Authors: Yang G. , Deng Y.‐J. , Qin H. , Zhu B.‐F. , Chen F. , Shen C.‐M. , Sun Z.‐M. , Chen L.‐P. , Wu J. , Mu H.‐F. , Lucas R. 
Published: March 2010

SummaryTo identify HLA‐B*15 subtypes distribution in Han population in Beijing, People’s Republic of China, 826 unrelated healthy individuals were typed using the polymerase chain reaction‐sequence‐based typing method. Within the 246 HLA‐B*15 positive individuals, 29 HLA‐B*15 alleles were identified, the most predominant of which is B*1501 (40.07%), followed by B*1502 (12.87%), B*1511 (12.87%), B*1518 (9.19%) and B*1532 (3.31%). The distribution of HLA‐B*15 subtype frequencies was compared between the Beijing Han, eight other Chinese ethnic minorities and six Chinese populations covering the mainland of China, Taiwan, Hong Kong and Singapore. A neighbor‐joining phylogenetic tree was constructed and revealed that the Beijing Han population clustered into the northern populations group and had a closer relationship with northern Han and Hui than with southern Han or other ethnic minorities. These results thus provide useful information that can be used in anthropology, selection for bone marrow transplantation as well as in disease‐association study, such as in carbamazepine (CBZ)‐induced Stevens–Johnson syndrome and toxic epidermal necrolysis.
Protecting River Corridors in Vermont1
Authors: Kline Michael , Cahoon Barry 
Published: March 2010

Kline, Michael and Barry Cahoon, 2010. Protecting River Corridors in Vermont. Journal of the American Water Resources Association (JAWRA) 46(2):227‐236. DOI: 10.1111/j.1752‐1688.2010.00417.xAbstract:  The Vermont Agency of Natural Resources’ current strategy for restoring aquatic habitat, water quality, and riparian ecosystem services is the protection of fluvial geomorphic‐based river corridors and associated wetland and floodplain attributes and functions. Vermont has assessed over 1,350 miles of stream channels to determine how natural processes have been modified by channel management activities, corridor encroachments, and land use/land cover changes. Nearly three quarters of Vermont field‐assessed reaches are incised limiting access to floodplains and thus reducing important ecosystem services such as flood and erosion hazard mitigation, sediment storage, and nutrient uptake. River corridor planning is conducted with geomorphic data to identify opportunities and constraints to mitigating the effects of physical stressors. Corridors are sized based on the meander belt width and assigned a sensitivity rating based on the likelihood of channel adjustment due to stressors. The approach adopted by Vermont is fundamentally based on restoring fluvial processes associated with dynamic equilibrium, and associated habitat features. Managing toward fluvial equilibrium is taking hold across Vermont through adoption of municipal fluvial erosion hazard zoning and purchase of river corridor easements, or local channel and floodplain management rights. These tools signify a shift away from primarily active management approaches of varying success that largely worked against natural river form and process, to a current community‐based, primarily passive approach to accommodate floodplain reestablishment through fluvial processes.
Groundwater Denitrification Capacity of Riparian Zones in Suburban and Agricultural Watersheds1
Authors: Watson Tara K. , Kellogg Dorothy Q. , Addy Kelly , Gold Arthur J. , Stolt Mark H. , Donohue Sean W. , Groffman Peter M. 
Published: March 2010

Watson, Tara K., Dorothy Q. Kellogg, Kelly Addy, Arthur J. Gold, Mark H. Stolt, Sean W. Donohue, and Peter M. Groffman, 2010. Groundwater Denitrification Capacity of Riparian Zones in Suburban and Agricultural Watersheds. Journal of the American Water Resources Association (JAWRA) 46(2):237‐245. DOI: 10.1111/j.1752‐1688.2010.00418.xAbstract:  We evaluated the relationship of dominant watershed land use to the structure and nitrogen (N) sink function of riparian zones. We focused on groundwater denitrification capacity, water table dynamics, and the presence and pattern of organically enriched deposits. We used the push‐pull method (measurement of 15N‐enriched denitrification gases derived from an introduced groundwater plume of 15N‐enriched nitrate) to evaluate groundwater denitrification capacity on nine forested wetland riparian sites developed in alluvial or outwash parent materials in southern New England. Three replicate sites were located in each of the three watershed types, those with substantial (1) irrigated agriculture, (2) suburban development, and (3) forest. Soil morphology and water table dynamics were assessed at each site. We found significantly lower mean annual water tables at sites within watersheds with substantial irrigated agriculture or suburban development than forested watersheds. Water table dynamics were more variable at sites within suburban watersheds, especially during the summer. Groundwater denitrification capacity was significantly greater at sites within forested watersheds than in watersheds with substantial irrigated agriculture. Because of the high degree of variability observed in riparian sites within suburban watersheds, groundwater denitrification capacity was not significantly different from either forested or agricultural watersheds. The highly variable patterns of organically enriched deposits and water tables at sites within suburban watersheds suggests that depositional events are irregular, limiting the predictability of groundwater N dynamics in these riparian zones. The variability of riparian N removal in watersheds with extensive suburbia or irrigated agriculture argues for N management strategies emphasizing effective N source controls in these settings.
Hot Spots and Hot Moments in Riparian Zones: Potential for Improved Water Quality Management1
Authors: Vidon Philippe , Allan Craig , Burns Douglas , Duval Tim P. , Gurwick Noel , Inamdar Shreeram , Lowrance Richard , Okay Judy , Scott Durelle , Sebestyen Steve 
Published: March 2010

Vidon, Philippe, Craig Allan, Douglas Burns, Tim P. Duval, Noel Gurwick, Shreeram Inamdar, Richard Lowrance, Judy Okay, Durelle Scott, and Steve Sebestyen, 2010. Hot Spots and Hot Moments in Riparian Zones: Potential for Improved Water Quality Management. Journal of the American Water Resources Association (JAWRA) 46(2):278‐298. DOI: 10.1111/j.1752‐1688.2010.00420.xAbstract:  Biogeochemical and hydrological processes in riparian zones regulate contaminant movement to receiving waters and often mitigate the impact of upland sources of contaminants on water quality. These heterogeneous processes have recently been conceptualized as “hot spots and moments” of retention, degradation, or production. Nevertheless, studies investigating the importance of hot phenomena (spots and moments) in riparian zones have thus far largely focused on nitrogen (N) despite compelling evidence that a variety of elements, chemicals, and particulate contaminant cycles are subject to the influence of both biogeochemical and transport hot spots and moments. In addition to N, this review summarizes current knowledge for phosphorus, organic matter, pesticides, and mercury across riparian zones, identifies variables controlling the occurrence and magnitude of hot phenomena in riparian zones for these contaminants, and discusses the implications for riparian zone management of recognizing the importance of hot phenomena in annual solute budgets at the watershed scale. Examples are presented to show that biogeochemical process‐driven hot spots and moments occur along the stream/riparian zone/upland interface for a wide variety of constituents. A basic understanding of the possible co‐occurrence of hot spots and moments for a variety of contaminants in riparian systems will increase our understanding of the influence of riparian zones on water quality and guide management strategies to enhance nutrient or pollutant removal at the landscape scale.
Antidiabetic activity of the mangrove speciesCeriops decandrain alloxan‐induced diabetic rats
Authors: NABEEL Mannalamkunnath Alikunhi , KATHIRESAN Kandasamy , MANIVANNAN Subramanian 
Published: March 2010

AbstractBackground: Diabetes is a series of disorders characterized by increased fasting and postprandial glucose concentration and insulin deficiency and/or decreased insulin action. Although there are a number of commercially available drugs for the treatment of diabetes, their long‐term use may cause unwanted side effects. Consequently, many studies are underway to find natural remedies that can effectively reduce the intensity of diabetes. The aim of the present study was to evaluate the antidiabetic activity of the mangrove speciesCeriops decandra.Methods: The effects of daily oral administration of an ethanolic extract from the leaves ofC. decandra(30, 60, 120 mg/kg) for 30 days on blood glucose, hemoglobin (Hb), HbA1c, liver glycogen and some carbohydrate metabolic enzymes were evaluated in normal and alloxan‐induced diabetic rats. The effects of these extracts were compared with the effect of 30‐days treatment with 0.1 mg/kg, p.o., glibenclamide, a commercially available drug commonly used in the treatment of diabetes.Results: Oral administration of 120 mg/kg extract modulated all the parameters evaluated to levels seen in control rats. The effects of 120 mg/kg extract were comparable to those of glibenclamide.Conclusion: The extract of the mangrove plantC. decandraexhibited promising antidiabetic activity and could be considered for further evaluation in clinical studies and drug development.
Measurement of HbA1c from stored whole blood samples in the Atherosclerosis Risk in Communities study
Authors: SELVIN Elizabeth , CORESH Josef , ZHU Hong , FOLSOM Aaron , STEFFES Michael W. 
Published: March 2010

AbstractBackground:  The aims of the present study were to demonstrate the reliability of HbA1c measurements during two time periods and to compare these measurements with HbA1c distribution in the general US population.Methods:  HbA1c was measured in 14 069 whole blood samples in the Atherosclerosis Risk in Communities (ARIC) study using different HPLC instruments across two time periods, namely 2003–2004 and 2007–2008. At the time of measurement, samples had been in storage at −70°C for up to 18 years. To assess differences in values, HbA1c measurements were repeated in 383 samples at both periods. Indirect comparisons were made by comparing our measurements against those from a nationally representative study.Results:  The coefficients of variation for quality control samples were 1.8% (n = 89) in 2003–2004 and 1.4% (n = 259) in 2007–2008. The correlation between measurements at the two time points was high (r = 0.99), but with a slight bias: 0.29% points higher in 2007–2008 vs 2003–2004 (n = 383; P < 0.0001). The comparison yielded the following Deming regression equation: y(2007–2008) = 0.073 + 1.034x(2003–2004). After alignment using this equation, the distribution of HbA1c in the ARIC study was similar to that in the national study using fresh samples.Conclusions:  Measurements of HbA1c from samples stored for up to 18 years are highly reliable when using state‐of‐the‐art HPLC instruments, but with some bias introduced over time. The HbA1c data now available in the ARIC study should be invaluable for investigations into the clinical utility of HbA1c as a diagnostic test for diabetes.