bluuebunny/crossref_metadata_2025_split huggingface dataset:
Being less of a man or less of a woman: Perceptions of chronic pain patients’ gender identities
Authors: Bernardes Sónia F. , Lima Maria Luísa 
Published: May 2009

AbstractLiving with chronic pain may be a threatening experience to one's own gender identity. Findings suggest that the presence of chronic pain does not allow individuals to achieve the most valued standards of being male or female in our societies. Such contention, however, has not yet been empirically supported. Therefore, our goal was to explore laypeople's and nurses’ perceptions of the man/woman with chronic low‐back pain (CLBP) as compared to the typical man/woman, respectively. Three hundred and sixteen laypeople (52.8% women) and 161 nurses (54% women) participated in this study. Half of the participants were presented with a written vignette depicting a man/woman with CLBP, followed by a list of 33 traits of the masculine and feminine stereotypes. Participants evaluated the extent to which each trait fit their image of the man/woman with CLBP. The other half of the participants described the image people in general had of the typical man/woman using the same list of traits. This study consisted on a quasi‐experimental design, 2 (character's sex) × 2 (type of character) × 2 (participant's sex) × 2 (health‐care training). Results have generally supported our hypotheses. Both laypeople and nurses perceived: (1) the man with CLBP as having less masculinity and more femininity‐related traits than the typical man; (2) the woman with CLBP as having less femininity and more masculinity‐related traits than the typical woman; and (3) the man and woman with CLBP as more similar to each other than the typical man/woman. Issues on gender identity conflicts in CP patients are discussed.
Characterization of a QTL region affecting clinical mastitis and protein yield on BTA6
Authors: Nilsen H. , Olsen H. G. , Hayes B. , Nome T. , Sehested E. , Svendsen M. , Meuwissen T. H. E. , Lien S. 
Published: May 2009

SummaryQuantitative trait loci affecting clinical mastitis were detected and fine mapped to a narrow region on bovine chromosome 6 in the Norwegian Red cattle population. The region includes the casein gene cluster and several candidate genes thought to influence clinical mastitis. The most significant results were found for SNPs within the Mucin 7 gene. This gene encodes an antimicrobial peptide and constitutes part of the first line of defence for the mucosal immune system. Detection of long haplotypes extending several Mb may indicate that artificial selection has influenced the haplotype structures in the region. A search for selection sweeps supports this observation and coincides with association results found both by single SNP and haplotype analyses. Our analyses identified haplotypes carrying quantitative trait loci alleles associated with high protein yield and simultaneously fewer incidences of clinical mastitis. The fact that such haplotypes are found in relative high frequencies in Norwegian Red may reflect the combined breeding goal that is characterized by selection for both milk production and disease resistance. The identification of these haplotypes raises the possibility of overcoming the unfavourable genetic correlation between these traits through haplotype‐assisted selection.
Quantitative trait loci associated with fatness in a broiler–layer cross
Authors: Campos R. L. R. , Nones K. , Ledur M. C. , Moura A. S. A. M. T. , Pinto L. F. B. , Ambo M. , Boschiero C. , Ruy D. C. , Baron E. E. , Ninov K. , Altenhofen C. A. B. , Silva R. A. M. S. , Rosário M. F. , Burt D. W. , Coutinho L. L. 
Published: May 2009

SummaryAn F2 population established by crossing a broiler male line and a layer line was used to map quantitative trait loci (QTL) affecting abdominal fat weight, abdominal fat percentage and serum cholesterol and triglyceride concentrations. Two genetic models, the line‐cross and the half‐sib, were applied in the QTL analysis, both using the regression interval method. Three significant QTL and four suggestive QTL were mapped in the line‐cross analysis and four significant and four suggestive QTL were mapped in the half‐sib analysis. A total of five QTL were mapped for abdominal fat weight, six for abdominal fat percentage and four for triglyceride concentration in both analyses. New QTL associated with serum triglyceride concentration were mapped on GGA5, GGA23 and GG27. QTL mapped between markers LEI0029 and ADL0371 on GGA3 for abdominal fat percentage and abdominal fat weight and a suggestive QTL on GGA12 for abdominal fat percentage showed significant parent‐of‐origin effects. Some QTL mapped here match QTL regions mapped in previous studies using different populations, suggesting good candidate regions for fine‐mapping and candidate gene searches.
Mapping quantitative trait loci regulating chicken body composition traits
Authors: Gao Y. , Du Z. Q. , Wei W. H. , Yu X. J. , Deng X. M. , Feng C. G. , Fei J. , Feng J. D. , Li N. , Hu X. X. 
Published: May 2009

SummaryGenome scans were conducted on an F2 resource population derived from intercross of the White Plymouth Rock with the Silkies Fowl to detect QTL affecting chicken body composition traits. The population was genotyped with 129 microsatellite markers and phenotyped for 12 body composition traits on 238 F2 individuals from 15 full‐sib families. In total, 21 genome‐wide QTL were found to be responsible for 11 traits, including two newly studied traits of proventriculus weight and shank girth. Three QTL were genome‐wide significant: at 499 cm on GGA1 (explained 3.6% of phenotypic variance, P < 0.01) and 51 cm on GGA5 (explained 3.3% of phenotypic variance, P < 0.05) for the shank & claw weight and 502 cm on GGA1 (explained 1.4% of phenotypic variance, P < 0.05) for wing weight. The QTL on GGA1 seemed to have pleiotropic effects, also affecting gizzard weight at 490 cm, shank girth at 489 cm and intestine length at 481 cm. It is suggested that further efforts be made to understand the possible pleiotropic effects of the QTL on GGA1 and that on GGA5 for two shank‐related traits.
A splice site single nucleotide polymorphism of the fatty acid binding protein 4 gene appears to be associated with intramuscular fat deposition in longissimus muscle in Australian cattle
Authors: Barendse W. , Bunch R. J. , Thomas M. B. , Harrison B. E. 
Published: May 2009

Summary Fatty acid binding protein 4 (FABP4) is a candidate gene affecting fatness traits of mammals. However, its association with fatness traits in cattle and other livestock species is not consistent from one study to another. Here, we sequenced the coding sequence of FABP4 looking for non‐synonymous variants. We identified a splice site mutation between the third exon and the third intron of bovine FABP4. We genotyped this SNP, FABP4:g.2502C>G, in 1409 cattle with intramuscular fat measurements from seven breeds. The average allele frequency of the C allele was 0.66 with a range of 0.45 to 0.85. A regression on the number of G alleles shows a statistically significant effect of α = 0.11, P = 0.044. This appears to confirm an association between IMF and variation at FABP4, with an effect of 0.3% of the variation in our sample when using this SNP.
Identification of chromosomal regions associated with growth and carcass traits in an F3 full sib intercross line originating from a cross of chicken lines divergently selected on body weight
Authors: Terčič D. , Holcman A. , Dovč P. , Morrice D. R. , Burt D. W. , Hocking P. M. , Horvat S. 
Published: May 2009

SummaryAn F3 resource population originating from a cross between two divergently selected lines for high (D+ line) or low (D− line) body weight at 8‐weeks of age (BW55) was generated and used for Quantitative Trait Locus (QTL) mapping. From an initial cross of two founder F0 animals from D(+) and D(−) lines, progeny were randomly intercrossed over two generations following a full sib intercross line (FSIL) design. One hundred and seventy‐five genome‐wide polymorphic markers were employed in the DNA pooling and selective genotyping of F3 to identify markers with significant effects on BW55. Fifty‐three markers on GGA2, 5 and 11 were then genotyped in the whole F3 population of 503 birds, where interval mapping with GridQTL software was employed. Eighteen QTL for body weight, carcass traits and some internal organ weights were identified. On GGA2, a comparison between 2‐QTL vs. 1‐QTL analysis revealed two separate QTL regions for body, feet, breast muscle and carcass weight. Given co‐localization of QTL for some highly correlated traits, we concluded that there were 11 distinct QTL mapped. Four QTL localized to already mapped QTL from other studies, but seven QTL have not been previously reported and are hence novel and unique to our selection line. This study provides a low resolution QTL map for various traits and establishes a genetic resource for future fine‐mapping and positional cloning in the advanced FSIL generations.
Effects of leptin Arg25Cys on peripheral mononuclear cell counts and antibody response to vaccination in beef cattle
Authors: Asiamah P. A. , Bechtel D. G. , Waldner C. , Buchanan F. C. 
Published: May 2009

SummaryA single nucleotide polymorphism (SNP) in the leptin gene that results in Arg25Cys has been associated with beef carcass quality and milk composition in dairy cattle. However, leptin (LEP) also plays a role in immune performance and hence it was important to determine whether selection based on this SNP would negatively affect immune cell numbers or antibody production. LEP c.73C>T was assessed for effects on immune cell counts and antibody titres in 27 beef cattle herds (n = 556). A commercial rabies vaccine had been administered to these animals. Prior to being vaccinated, counts of several important mononuclear cells (total and activated B lymphocytes, total and activated T helper and T cytotoxic, WC1 T lymphocytes and monocytes) as well as baseline serum antibody titres were determined for each animal. On day 21, antibody titres were measured and a booster vaccine was administered. Finally on day 42, antibody titres and mononuclear cell types were again counted. Counts of six different cell types were significantly associated with the LEP genotype; however, no consistent patterns were observed between LEP genotype (TT, CT or CC) and peripheral blood mononuclear cell populations. Significant differences in the production of rabies antibodies in response to vaccination were observed relative to LEP genotype. Our results suggest that selection for either the C or T allele would not detrimentally impact on the measured indicators of immune function in beef cattle.
Expanded safety experience with lenalidomide plus dexamethasone in relapsed or refractory multiple myeloma
Authors: Chen Christine , Reece Donna E. , Siegel David , Niesvizky Ruben , Boccia Ralph V. , Stadtmauer Edward A. , Abonour Rafat , Richardson Paul , Matous Jeffrey , Kumar Shaji , Bahlis Nizar J. , Alsina Melissa , Vescio Robert , Coutre Steven E. , Pietronigro Dennis , Knight Robert D. , Zeldis Jerome B. , Rajkumar Vincent 
Published: May 2009

SummaryLenalidomide gained Food and Drug Administration (FDA) approval for treatment of patients with relapsed or refractory multiple myeloma (MM) in combination with dexamethasone in June 2006. In April 2005, the FDA and patient advocacy groups requested an expanded access programme to both provide lenalidomide to patients likely to benefit and obtain additional safety information. Relapsed/refractory MM patients received lenalidomide 25 mg/d (days 1–21) and dexamethasone 40 mg/d (days 1–4, 9–12, and 17–20 of cycles 1–4; days 1–4 only from cycle 5 onwards), in 4‐week cycles until disease progression, study drug discontinuation, or lenalidomide approval. Of the 1438 patients enrolled, ∼60% were male, median age was 64 years, and 61·7% had Durie‐Salmon stage III disease. Median time on study was 15·4 weeks (range: 0·1–49·1) and median dose was 25 mg. The most common adverse events (AEs) were haematological (49%), gastrointestinal (59%), and fatigue (55%). The most common grade ≥3 AEs were haematological (45%), fatigue (10%), and pneumonia (7%). The most common serious AEs were pneumonia (8%), pyrexia (4%), and deep‐vein thrombosis (3%). Primary cause of death was disease progression (10%). Safety data confirmed known AEs of lenalidomide plus dexamethasone therapy in patients with relapsed/refractory MM.
Distribution of NPM1‐ALK and X‐ALK fusion transcripts in paediatric anaplastic large cell lymphoma: a molecular–histological correlation
Authors: Damm‐Welk Christine , Klapper Wolfram , Oschlies Ilske , Gesk Stefan , Röttgers Silja , Bradtke Jutta , Siebert Reiner , Reiter Alfred , Woessmann Willi 
Published: May 2009

SummaryAnaplastic large cell lymphomas (ALCL) in children express anaplastic lymphoma kinase (ALK) fusion genes, most commonly NPM1‐ALK. The distribution of X‐ALK among 66 childhood ALCLs was analysed. One ALCL was ALK‐negative. Reverse transcription polymerase chain reaction detected NPM1‐ALK in 58 tumours, all showing nuclear and cytoplasmic ALK staining. The remaining seven ALCL stained for ALK in the cytoplasm only: two expressed TPM3‐ALK, one ATIC‐ALK, one MYH9‐ALK; three no TPM3‐, TFG‐, ATIC‐, CLTC‐ or MYH9‐ALK. Almost 90% of paediatric ALK‐positive ALCLs express NPM1‐ALK. There was complete concordance between ALK staining pattern and the presence of a typical/variant ALK fusion partner.
The same annual dose of 292000 IU of vitamin D3 (cholecalciferol) on either daily or four monthly basis for elderly women: 1‐year comparative study of the effects on serum 25(OH)D3 concentrations and renal function
Authors: Pekkarinen Tuula , Välimäki Ville‐Valtteri , Aarum Stiina , Turpeinen Ursula , Hämäläinen Esa , Löyttyniemi Eliisa , Välimäki Matti J 
Published: May 2009

SummaryObjective  Daily dosing of vitamin D supplements may be difficult among older people. Infrequent administration of ‘megadoses’ controlled by health care personnel may overcome adherence problem. We compared the efficacy and safety of two oral dosages (800 IU daily or 97333 IU four monthly) of vitamin D3 resulting in the equal annual dose of 292000 IU.Design  Randomized, double‐blind, double‐dummy parallel group comparison.Patients  Forty women aged 69·3–78·8 years.Interventions  Vitamin D3 400 IU twice daily (D group) or vitamin D3 oil 97333 IU every 4 months (4 M group) for 1 year. All received 1 g of calcium daily.Measurements  Serum 25‐hydroxyvitamin D3 [25(OH)D3] in relation to the target levels of 50–75 nmol/l, PTH, serum type I procollagen aminoterminal propeptide (PINP), serum and urine calcium, renal function.Results  A quantity of 25OHD3 increased more in D group than in 4 M group (P < 0·0001). All participants in D group and 67% in 4 M group had 25(OH)D3 above 50 nmol/l at 12 months; the target level of 75 nmol/l was reached by 47% and 28% respectively. PTH did not show any seasonal perturbation in either group. PINP declined and urinary calcium rose similarly in the study groups over time (P < 0·0001). Renal function did not worsen in either group.Conclusions  In terms of serum 25(OH)D3 concentrations, 800 IU daily was more efficient than a 97333 IU every 4 months. However, to increase adherence, the latter is still worth developing. Both treatments increased urinary excretion of calcium, but did not worsen renal function.
Insulin sensitivity and secretion in children and adolescents with hypothalamic obesity following treatment for craniopharyngioma
Authors: Simoneau‐Roy Judith , O’Gorman Clodagh , Pencharz Paul , Adeli Khosrow , Daneman Denis , Hamilton Jill 
Published: May 2009

SummaryBackground  Craniopharyngioma (CP), a tumour occurring in the hypothalamic–pituitary area, results in morbid obesity in 25–60% of affected children. It has been suggested that abnormalities of insulin secretion and/or insulin action due to hypothalamic injury may be associated with weight gain and the metabolic syndrome in this population.Aim  To evaluate: (i) insulin secretion (IS) and insulin sensitivity (Si); (ii) features of the metabolic syndrome (MS) and (iii) factors involved in risk for diabetes and heart disease in obese youth treated for CP.Methods  Obese subjects treated for CP were compared to BMI‐matched control subjects. All subjects underwent anthropometric, blood pressure, resting energy expenditure and body fat assessment. Cholesterol and inflammatory markers, oral glucose tolerance test (OGTT) and frequent sampling intravenous glucose tolerance test (FSIGT), with calculation of IS and Si, were performed.Results  Fifteen CP subjects and 15 controls (C) were studied. There were no differences between CP and C for age, gender, BMI or pubertal status. MS was present in 10/15 CP and 3/15 C (P = 0·03), including impaired glucose tolerance (IGT) in 6/15 CP and 0/15 C (P = 0·02). Measures of IS, including first and second phase IS, and insulin area‐under‐the‐curve (AUCins) during OGTT, were significantly higher in CP. Si, measured by frequent sampled intravenous glucose tolerance test (Si‐FSIGT), was significantly lower in CP subjects (0·96 ± 0·34 vs. 1·67 ± 0·7; P = 0·01). AUCins was negatively correlated with Si‐FSIGT (r = −0·62; P = 0·003).Conclusion  Children with CP and hypothalamic obesity have more features of MS, increased IS and IGT prevalence and lower Si compared with BMI‐matched controls.
Initial thyroid status and cardiovascular risk factors: The EPIC‐Norfolk prospective population study
Authors: Boekholdt S. Matthijs , Titan Silvia M. , Wiersinga Wilmar M. , Chatterjee Krishna , Basart Dick C.G. , Luben Robert , Wareham Nicholas J. , Khaw Kay‐Tee 
Published: May 2009

SummaryContext  Thyroid status affects several aspects of cardiovascular risk profile, including lipid levels and blood pressure. Whether thyroid status affects the risk of coronary heart disease (CHD) and all‐cause mortality remains controversial.Design  The EPIC‐Norfolk prospective population study. Mean follow‐up was 10·6 years.Patients  Study participants were 11 554 men and women aged 45–79 years, who were living in Norfolk, UK.Measurements  Baseline cardiovascular risk factors were recorded and concentrations of thyroid‐stimulating hormone (TSH) and free thyroxine (FT4) were measured in baseline samples. Regression analyses were performed to assess the association between thyroid hormone levels and cardiovascular risk factors. A proportional hazards model was used to estimate the risk of CHD and all‐cause mortality by baseline thyroid status. No information was available on thyroid treatment during follow‐up.Results  Thyroid abnormalities were common, particularly among women. Thyroid abnormalities were associated with an altered cardiovascular risk profile. Even within the normal range, thyroid hormone levels, TSH more strongly than FT4, were associated with lipid levels and blood pressure among both men and women. We did not observe a significant association between subclinical thyroid abnormalities and risk of CHD or all‐cause mortality.Conclusions  Despite the association between thyroid hormone levels and cardiovascular risk factors, thyroid status was not statistically significantly associated with the risk of future CHD or all‐cause mortality in this large cohort.
Thrombin generation in haemophilia A patients with factor VIII inhibitors after infusion of recombinant factor VIIa
Authors: Eichinger S. , Lubsczyk B. , Kollars M. , Traby L. , Zwiauer K. , Gleiss A. , Quehenberger P. , Kyrle P. A. 
Published: May 2009

AbstractBackground  Development of factor VIII inhibitors is a serious complication in haemophilia A patients. Recombinant factor VIIa (rVIIa) is clinically effective, but its effects on haemostatic system need still to be fully elucidated.Material and methods  In an open controlled study, we measured thrombin generation (peak thrombin) in venous blood and prothrombin fragment F1 + 2 (F1 + 2) and d‐dimer in venous and in shed blood in five haemophilia A patients with inhibitors before and after rVIIa infusion. A total of five healthy individuals who did not receive rVIIa served as controls.Results  At baseline, patients had lower mean (min‐max) peak thrombin levels than controls [0·12 (0·0–0·6) vs. 186·9 (116·0–254·4) nM, P = 0·001]. After infusion, peak thrombin levels increased in average to 40·7 (28·3–51·6) nM, which translates into 80·2% (95% CI 65·4–88·6%) lower levels compared to that of controls. Mean (min‐max) F1 + 2 levels in venous blood did not differ significantly between patients and controls [160·7 (89·8–331·3) vs. 160·8 (104·4–242·3) pmol L−1], but increased in average (min‐max) by 39·4% (14·1–58·5%) after infusion. In blood emerging from incisions made to determine the bleeding (shed blood), F1 + 2 levels were lower in patients than controls [1383·3 (906·4–2044·6) vs. 2981·7 (1610·0–4539·6) pmol L−1; P = 0·04], but were not affected by rVIIa; d‐dimer levels were significantly higher in haemophiliacs than in controls and remained unchanged after infusion.Conclusions  Haemophilia A patients with factor VIII inhibitors have low thrombin generation. After rVIIa, the extent of coagulation activation as measured by levels of F1 + 2 is increased, but thrombin generation is restored to only 20%. Peak thrombin levels could reflect the effects of rVIIa on coagulation mechanisms, and their relevance with regard to the clinical coagulation defect of haemophilia A patients with factor VIII inhibitors might be evaluated.
First isolation of Tenacibaculum maritimum from wedge sole, Dicologoglossa cuneata (Moreau)
Authors: López J R , Núñez S , Magariños B , Castro N , Navas J I , De La Herran R , Toranzo A E 
Published: May 2009

AbstractThe first isolation of Tenacibaculum maritimum from wedge sole, Dicologoglossa cuneata, is reported. The pathogen was recovered from ulcers of cultured fish, from three different outbreaks. The six isolates obtained were biochemically and serologically characterized and diagnosis was confirmed by polymerase chain reaction using specific primers and partial 16S rRNA gene sequencing. The isolates constituted a homogeneous phenotypic group; however, they belong to two of the different serotypes described within this species. A virulence evaluation of the isolates using Wedge sole fry was also performed.
Voluntary dehydration among elementary school children residing in a hot arid environment
Authors: Bar‐David Y. , Urkin J. , Landau D. , Bar‐David Z. , Pilpel D. 
Published: May 2009

AbstractBackground:  Voluntary dehydration is a condition where humans do not drink appropriately in the presence of an adequate fluid supply. This may adversely affect their physical and intellectual performance. The present study aimed to describe the prevalence of voluntary dehydration among elementary school children of different ethnicities and countries of birth.Methods:  Four hundred and twenty‐nine elementary school children, aged 8–10 years, from four subpopulations (Israeli‐born Jewish and Bedouin‐Arab children, and immigrant children who recently arrived to Israel from Eastern Europe and from Ethiopia) were studied. The level of dehydration was determined by noontime urine osmolality, from samples taken over 1 week in mid‐summer. Urine osmolality <500 mOsmol kg−1 H2O was considered to be an appropriate level of hydration.Results:  Mean urine osmolality was 862 ± 211 mOsmol kg−1 H2O. Osmolality above 800 mOsmol kg−1 H2O was detected in 67.5% of the urine samples; among these, 25% were above 1000 mOsmol kg−1 H2O. The most dehydrated group was that of Israeli‐born Jewish children, whereas the Bedouin‐Arab children were the least dehydrated.Conclusions:  A high proportion of children who reside in a hot and arid environment were found to be in a state of moderate to severe dehydration. Bedouin ethnicity was associated with better hydration, whereas Israeli‐born Jews were most severely dehydrated. Educational intervention programmes promoting water intake should start in early childhood and continue throughout life.