bluuebunny/crossref_metadata_2025_split huggingface dataset:
Helicobacter pylori detection in gastric biopsy: immunohistochemistry and toluidine blue comparison
Authors: Paramita Ayu Lidya , Setiawan Firman , Sandhika Willy , Mertaniasih Ni Made , Kawilarang Arthur Pohan , Utomo Budi 
Published: May 2024

Link of Video Abstract: https://youtu.be/E3KoLryYLng

Background: Helicobacter pylori is a bacterium often found in the stomach and can cause several diseases, including gastritis, gastric ulcers, MALT-lymphoma, and gastric carcinoma. An invasive and direct gastric biopsy is a more appropriate way to see the presence of these bacteria. Microscopy of Helicobacter pylori on gastric biopsy specimens can be seen using several tissue stains. This study aimed to analyze the concordance of immunohistochemistry and toluidine blue tissue stain results on gastric biopsies in detecting Helicobacter pylori at Dr. Soetomo Hospital.
Methods: This study was a retrospective study with a cross-sectional design to detect Helicobacter pylori in paraffin-block of gastric biopsy specimens at Dr. Soetomo Hospital, Surabaya. Each sample would be stained by immunohistochemistry and toluidine blue. Data were analyzed using SPSS version 25 for Windows.
Results: Helicobacter pylori have been found in all 45 samples by immunocytochemical staining and in 66.67% of them by toluidine blue staining. The Kappa test indicated a significant difference between immunohistochemical staining and toluidine blue (p=0.000; p<0.05).
Conclusion: There are significant differences between the two methods for detecting Helicobacter pylori in gastric biopsy. Their concordance is at 66,67%. Some factors should be considered for the future routine process.
The important role of multi-sector partnership in stunting management in east java: a literature review
Authors: Dini Setiarsih  , Sa'bania Hari Raharjeng  , Rizki Nurmalya Kardina  , Paramita Viantri  , Fildzah  , Pratiwi Hariyani Putri  , Achmad Syafiuddin  , Rizki Amalia  , Karina Widowati  
Published: May 2024

In order to integrate cross-sectoral activities in stunting prevention, the East Java provincial government developed a Regional Action Plan for Food and Nutrition (RAPFN). The integration of multi-sectoral activities is predicted to be leveraged in stunting prevention. This study aims to obtain an overview of the important role of multi-sectoral partnerships in stunting prevention in East Java. This study is a literature review with the stages of problem formulation, literature search, data extraction and data analysis and interpretation. The results of the search and study show that the East Java Provincial Government partnerships are carried out with non-governmental institutions, including universities, civil society organizations, companies, and the community itself. Partner universities include Universitas Nahdlatul Ulama Surabaya, Universitas Airlangga, and Universitas Jember. Partners from civil society organizations include various non-profit organizations coordinated in one program such as Micronutrient Supplementation for Reducing Mortality and Morbidity (MSRMM), initiated by Nutrition International and organizations with their programs. Partners from private companies are also under the direction of the government and among them is a program coordinated by PT Indofood Sukses Makmur Tbk. With the diverse backgrounds of partners, the programs that are run can vary but remain integrated with the direction of the government. The government's partnership with non-government institutions is expected to be a leverage to accelerate stunting reduction.
Acute Lymphoblastic Leukemia (B-ALL) with co-expression of CD33 and CD13
Authors: Kustiah  , Hernaningsih Yetti 
Published: May 2024

Link of Video Abstract: https://www.youtube.com/watch?v=AC472eZBXII

Background: Most acute leukemias can be classified into myeloid or lymphoid strains based on the expression of several specific phenotypes. However, some cases of acute leukemia express both phenotypes (myeloid and lymphoid phenotypes). To establish the diagnosis of acute leukemia with co-expression of myeloid markers or mixed phenotype acute leukemia (MPAL), we need a system that can differentiate between the two, namely by using the scoring system from The European Group for the Immunological Characterization of Leukemias (EGIL) and The World Health Organization (WHO) classification of tumors of the hematopoietic and lymphoid tissues 2016.
Case Presentation: A 10-year-old boy was admitted to the hospital because of repeated nosebleeds for 3 weeks before entering the hospital. Patients also complain of decreased appetite, nausea, vomiting, and joint pain. On physical examination, anemic conjunctiva was found, and no abnormalities were found on other physical examinations. Hemoglobin examination: 8.2 g/dl, leukocytes 112.10×103/µl, platelets 10×103/µl. Examination of peripheral blood smear: acute lymphoblastic leukemia. Examination results of bone marrow aspiration acute lymphoblastic leukemia (ALL L1). Immunophenotype analysis found blast cells with positive expression for CD34, CD79a, CD10, HLA-DR, CD33 and CD13.
Conclusion: The diagnosis in this patient was B-ALL with myeloid marker co-expression based on the EGIL and WHO scoring systems.
Risk factors and clinical outcomes of ventilator associated pneumonia in neonates in the Neonatal Intensive Care Unit of Dr. Soetomo Hospital Surabaya
Authors: Ratna Deni Widya , Ni Made Mertaniasih  , Risa Etika  , Pepy Dwi Endraswari  , Naritha Vermasari  
Published: May 2024

Link of Video Abstract: https://youtu.be/Si_Vw_cWchk

Introduction: Ventilator-associated pneumonia (VAP) is a hospital-acquired infection in mechanical ventilation for more than two consecutive days, resulting in increased mortality and treatment costs. This study aimed to analyse the risk factors and clinical outcomes of neonatal with ventilator associated pneumonia in the neonatal intensive care unit (NICU).
Methods: A case-control study was conducted on neonates who had mechanical ventilators installed for more than two consecutive days, from January 2020 to December 2021, at Dr. Soetomo Hospital, Surabaya. The risk factors studied were duration of stay in the NICU, reintubation, duration of ventilation before VAP, rate of prematurity, low birth weight, enteral feeding, and underlying disease. The clinical outcomes studied were the duration of hospital stay and the total ventilation duration.
Results: The study subjects were 135 neonates consisting of 45 cases of VAP and 90 non-VAP. Significant risk factors were duration of NICU stay (p<0.001), duration of ventilation before VAP (p=0.002), and rate of prematurity (p=0.024). Multivariate analysis found no independent risk factors for VAP. Hospital stay duration (p<0.001) and ventilation duration were significantly correlated with the incidence of VAP (p<0.001).
Conclusion: Risk factors for neonatal VAP were the duration of NICU stay, duration of ventilation prior to VAP occurrence, and degree of prematurity. The impact of VAP was longer duration of hospitalisation and duration of ventilation.
The effect of Chromolaena odorata on endometriosis implants in mice model of endometriosis
Authors: Rusnaidi  , Rajuddin  , Kamarlis Reno Keumalazia , Dasrul  
Published: May 2024

Introduction: Endometriosis is a benign gynecological disease whose pathogenesis mechanism is not fully understood with treatment modality with hormonal and surgical methods aiming to relieve symptoms, suppress progression, and increase fertility. This study aims to observe the differences in the administration of Chromolaena odorata leaf extract on the area of endometriosis implants in the mice model of endometriosis
Materials and Methods: This study was an experimental study with a Randomized Separated Pre and Post Test Design research conducted with the control group. Thirty mice (Mus musculus) were injected with 0.2 ml/mice of cyclosporine A. Then endometrial tissue was injected into the peritoneal cavity, followed by 0.1 ml Ethinyl estradiol/mice injections on the 1st and 5th days. Mice were reared for 14 days until the endometriosis model was formed. Mice models of endometriosis were randomly divided into five groups: endometriosis mice that were terminated on day 14 (pretest), then the other groups are endometriosis mice given Aquadest as placebo (P0), endometriosis mice treated with Chromolaena odorata leaf extract at a dose of 400 mg per kilograms of body weigh (mg/kgBW) (P1), 800 mg/kgBW (P2) and 1200 mg/kgBW (P3) for 14 days. On the twenty-eighth day, mice were dissected to examine and assess the extent of endometriosis implantation macroscopically in hyperemic areas by taking samples in the most hyperemic areas of the peritoneal wall. Research data were analyzed using SPSS Version 22.
Results: From this study, the average value of endometriosis implant area in the group given Chromolaena odorata leaf extract was 400 mg/kgBW (P1 = 15.60 mm2), 800 mg/kgBW (P2=14.23 mm2) and 1200 mg/kgBW (P3=0.00 mm2) was lower than the pretest group (P=104.72 mm2) and the placebo group (P0 = 153.89 mm2), showing a significant difference (p<0.001). There was a tendency that the area of endometriosis implants was also lower in the group receiving a higher dose of Chromolaena odorata leaf extract 1200 mg/kgBW (P3=0.00 mm2) than the group receiving a dose of 400 mg/kgBW (P1=15.60 mm2) and 800 mg/kgBW (P2=14.23 mm2).
Conclusions: It can be concluded that the administration of Chromolaena odorata leaf extract can reduce the area of endometriosis implants in mice models of endometriosis. Higher doses result in the lower endometrial implant area.
Effects of diabetes mellitus regulation on antibody response to inactivated virus vaccine: a systematic review
Authors: Prasetyaningtyas Dewi , Soegiarto Gatot , Wulandari Laksmi 
Published: May 2024

Background: Diabetes mellitus (DM) could hamper the immune responses, including that induced by the vaccine. The aim of this study was to evaluate the immunogenicity of coronavirus disease 2019 (COVID-19-19) inactivated vaccines in patients with DM either controlled or uncontrolled through a systematic review approach.
Methods: Without regard to time constraints, searches were conducted in ProQuest, Embase, and PubMed to assess the available data on the impact of DM regulation on the immunogenicity of post-vaccinated DM patients. The PRISMA guideline and the PICO criteria were used. The quality of studies was assessed using the National Institutes of Health (NIH) quality assessment tool. There is not enough Available data comparing the immunogenicity of the vaccine in controlled and uncontrolled DM patients is limited.
Results: Out of 20,657 articles, five studies were included, of which three had good quality and two had fair quality. Our data suggested that DM patients vaccinated with CoronaVac/SinoVac had lower seroconversion rates significantly lower compared to non-DM patients. The immunogenicity of BBV-152 vaccine in DM patients was also significantly lower than in patients without DM. Studies comparing the immunogenicity of the vaccine between controlled and uncontrolled DM patients are limited.
Conclusion: Patients with DM have lower antibody levels than non-DM patients who received inactivated COVID-19 patients. Glycemic management has emerged as critical to maximizing the immunogenicity induced by inactivated vaccines in a small number of patients; however, to support this conclusion, studies with adequate number of patients are critical.
Anxiety and perceived mental healthcare need among non-frontline in coronavirus referral hospital, Surabaya
Authors: Wijono Heru , Melaniani Soenarnatalina , Kuntoro  , Zakaria Zainul Amiruddin , Oka Made , Bte Marmaya Najihah Hanisah , Ikawaty Risma , Saputra Bagus Ida 
Published: May 2024

Link of Video Abstract: https://youtu.be/szoOkhYlQwY

Introduction: The pandemic of COVID-19 has put healthcare workers under high pressure with possible psychological problems and its most common effects are insomnia, depression, distress, anxiety, and somatization symptoms. Previous studies in several centers have shown different results between frontline and non-frontline healthcare workers. This study aims to evaluate the levels of anxiety and perceived needs for mental healthcare among non-frontline healthcare workers during the COVID-19 pandemic in a referral hospital in Surabaya.
Patients and methods: This cross-sectional study involved forty respondents, but one was discontinued due to technical problems. Questionnaire of The Hospital Anxiety and Depression Scale (HADS) was adopted that has 14 scales with two subscales. The subscales include HADS-A for Anxiety and HADS-D for depression
Results: The results show that both HADS-A and HADS-D have significant differences between the indirect and direct groups of contact healthcare workers, except in gender and depression, as shown in Table 3, with an average score of 8.000 (SD 5.02169) and 8.7333 (SD 4.71270), respectively.
Conclusion: Anxiety and depression remain a problem for all healthcare workers, whether frontline or non-frontline. Therefore, issues must be used to mitigate both the frontline and non-frontline healthcare workers.
Evaluation of hearing limits post tympanoplasty in patients with chronic suppurative otitis media
Authors: Probosutejo Nico , Ahadiah Titiek Hidajati 
Published: May 2024

Link of Video Abstract: https://youtu.be/Nr9MKgu2v2E

Background: Chronic suppurative otitis media (CSOM) causes partial or complete damage to the tympanic membrane and ossicles, causing hearing loss in the form of conductive and mixed hearing loss with a decrease of up to 60 dB. This study aimed to evaluate the hearing threshold before and after tympanoplasty surgery in CSOM patients.
Methods: This type of research was carried out in a retrospective descriptive manner. The materials in this study were the medical records of CSOM patients who underwent canal wall up (CWU) tympanoplasty and canal wall down (CWD) tympanoplasty at the outpatients of ear, nose, and throat – head and neck surgery (ENT-HNS) Otology Division Dr. Soetomo Hospital Surabaya for the period January 1, 2018 to December 31, 2018 with a total of 51 patients. This study analyzed with the Wilcoxon test.
Results: The mean age was 30.96 years (51% female and 49% male). Postoperatively 40 (78.5%) patients experienced an increase in hearing threshold, 10 (19.6%) patients experienced a decrease and 1 (1.9%) patient remained. The average increase in air-bone gap (ABG) pre and post tympanoplasty surgery was 9.78 dB, from 38.92 dB to 29.14 dB.
Conclusion: There was an increase in hearing threshold after tympanoplasty.
Platelet count, platelet lymphocyte ratio, and Ki67 as a predictive factor of neoadjuvant chemotherapy response in locally advanced breast cancer
Authors: Priyono Sasongko Hadi , Badriatunnor  , Meitria Syahadatina Noor  , Agung Ary Wibowo  , Didik Dwi Sanyoto  , Audi Ardansyah  
Published: May 2024

Link of Video Abstract: https://youtu.be/CjzggoIFnTU

Background: Locally advanced breast cancer (LABC) is a term that refers to advanced non metastatic stage breast cancer. Neoadjuvant chemotherapy is one of the therapies given to patients with LABC. Many factors can affect the chemotherapy response, platelets produce growth factors, and cytokines promote angiogenesis and tumor growth. Inflammatory biomarkers such as platelet lymphocyte ratio (PLR) may indicate tumor progressiveness. The Ki67 proliferation index is a marker of the aggressiveness or growth of tumor cells. This study aimed to determine the correlation of platelet count, platelet lymphocyte ratio and Ki67 proliferation index before chemotherapy with neoadjuvant chemotherapy response.
Method: This research used the cohort retrospective design method. A sample of 155 patients was selected using a simple random sampling technique. The inclusion criteria of this research are having complete data about pre-treatment platelet, lymphocyte platelet ratio, Ki67 and the neoadjuvant chemotherapy response result assessed by the oncologist that is written in the medical record. Patient data from other types of cancer, chronic, systemic or autoimmune diseases are excluded from this research. The data of each variable was analyzed with the Spearman correlation test.
Result: There was no correlation between platelet count and neoadjuvant chemotherapy response (p=0.301). There was a negative correlation between the platelet lymphocyte ratio (p=0.026) and the Ki67 index (p=0.040) with neoadjuvant chemotherapy response.
Conclusion: Based on this study, it can be concluded that platelet count is not significant in relation to chemotherapy responses. The higher the platelet lymphocyte ratio and the proliferation index of Ki67, the lower the response to neoadjuvant chemotherapy.
Characteristics of patients with nasopharyngeal carcinoma in Dr. Soetomo General Academic Hospital Surabaya
Authors: Utomo Audi Wahyu , Romdhoni Achmad Chusnu 
Published: May 2024

Link of Video Abstract: https://youtu.be/vwaMtqzZyI0

Background: Nasopharyngeal carcinoma (NPC) is a malignant tumor in the nasopharynx with a high incidence of metastasis. NPC is one of the most common cancers in Indonesia, and from the age distribution, it often affects the productive age population. This study aims to describe the characteristics of NPC patients in Dr.Soetomo General Academic Hospital from January 2017 – December 2018.
Methods: This study is a retrospective descriptive by taking medical records of patients with NPC at Dr. Soetomo Hospital, Surabaya, from January 2017-December 2018, that meet the inclusion and exclusion criteria. The basic data taken are gender, age, education, and occupation. Complaints assessed are the main complaints of patients who make patients come for treatment listed in the medical record data.
Results: Data from 262 patients showed that the five-year life expectancy for NPC sufferers in women is 84%, while in men, it is 78%. The main complaints of patients in this study were lumps in the neck in 130 patients (49.61%), with the highest distribution of patients based on stage IV a with 78 patients (29.77%), and the majority were non-keratinizing carcinoma undifferentiated type, with a total of 245 patients (93.51%).
Conclusion: Male sufferers are more than females; the largest age group is 41-50. Most jobs are private employees, and most sufferers' last education is high school. The main complaint of most patients was a lump in the neck, and the patient came with an advanced stage where the most found was stage IV. The histopathological type of most patients is non-keratinizing carcinoma undifferentiated type.
A novel solution for the patient with knee ankylosing spondylitis: a case report
Authors: Argo Risang Setyo , Kennedy Dave , Usman Muhammad Andry 
Published: May 2024

Link of Video Abstract: https://youtu.be/wskQdvBx0c0

Background: Knee ankylosis is a joint condition that causes functional limitation that becomes more severe when accompanied by pain. Arthroplasty is a complex and intricate reconstruction procedure that is more challenging when there is a structural limitation that requires a technique modification. In this report, we presented a rare case of a young patient with knee ankylosis and flexion contracture treated with arthroplasty.
Case Presentation: A 20-year-old young woman came to the hospital with stiffness in her left knee, inability to sit well and crouch, and difficulty getting up from sitting. Based on the physical examination and X-ray of the left knee, the patient showed bone ankylosis on the femorotibial joint with a 100 flexion, which confirmed knee ankylosis. Arthroplasty was performed with a parapatellar medial approach. After eight months of supervision, the patient was able to perform an active flexion within 1050 and a full extension on the left knee. In our patient, arthroplasty brought a satisfying functional outcome and pain reduction, which was also supported by previous studies with the same procedure on ankylosis patients.
Conclusion: Arthroplasty can be considered the main therapy option for joint deformities due to its more satisfying outcomes.
Relationship between hypoxia inducible factor-1 alpha (HIF-1α) levels and cellulite
Authors: Fovina Angela , Jusuf Nelva Karmila , Putra Imam Budi 
Published: May 2024

Link of Video Abstract: https://www.youtube.com/watch?v=ePouj9BkVdg

Background: Cellulite is a topographic change of the skin surface, characterized by an appearance like orange peel, mattress or cottage cheese, especially on the buttocks and hamstrings. Cellulite is a process that starts from adipocyte hypertrophy and dermal vascular damage, especially loss of the capillary network. Loss of the capillary network will cause the dermis and subcutaneous tissue to retain fluid, inhibiting venous return and causing swelling, ischemia, and hypoxia in the subcutaneous tissue. When hypoxia occurs, cellular adaptive processes activate hypoxia-inducible factor (HIF). Hypoxia-inducible factor-1α will interact with enzymes and other transcription factors to maintain homeostasis by increasing vascularization and tissue growth in acute hypoxic conditions. However, in chronic hypoxic conditions, HIF-1α can cause detrimental effects such as excessive extracellular matrix development resulting in tissue fibrosis. This study aims to determine the relationship between HIF-1α levels and cellulite.
Methods: This observational research included 40 cellulite patients and 40 controls in a cross-sectional design. Each patient got a medical history check, a dermatological exam, and blood testing to determine their HIF-1 levels using an ELISA test. The Chi-square test was used to statistically analyze these statistics.
Results: The mean HIF-1α level in cellulite was 5.74±2.39 ng/ml. The highest age with cellulite was 26–33 years, consisting of 12 (30%) people. Most of them had a family history of cellulite from their mother, consisting of 15 (37.5%) people. Nineteen (47.5%) of them were obese. Most cellulite is located on the femoral and gluteus regions in 27 people (67.5%). The result of this study showed that there was a relationship between high HIF-1α levels causing a risk of 4.8 times for cellulite (p=0.002).
Conclusion: There is a relationship between HIF-1α levels and cellulite.
Relationship between waist hip ratio index and photonumeric cellulite severity scale on cellulite
Authors: Lubis Ade Fitriyani , Jusuf Nelva Karmila , Putra Imam Budi 
Published: May 2024

Link of Video Abstract: https://youtu.be/ytotD9AJQPE

Introduction: Cellulite is one of many alterations in the physiology of the skin that obesity is linked to. Waist circumference measurement, in addition to body mass index (BMI), is another method for identifying obesity and assessing the risk of disease associated with body weight. The waist hip ratio (WHR), which is calculated by adding the measurements of the waist and hips, is a tool for identifying the kind of obesity. We sought to ascertain how the WHR index and PCSS related to cellulite.
Method: Based on inclusion and exclusion criteria, this study was an observational analytical study using a cross-sectional methodology with 40 female cellulite patients. Age and family background were noted. A measuring tape was used to determine the WHR index value. The PCSS scale is used to assess the severity of cellulite. Gamma correlation analysis was used to examine the link between the WHR index and PCSS; a relationship was considered significant if the p-value was less than 0.05.
Results: Cellulite occurs more frequently in women aged 26–35 years (37.5%) who have a family history of cellulite (82.5%) and WHR index values ≥ 0.85 (45%) with a mild degree of severity based on PCSS, totalling 20 people (50%). The findings of the study revealed a significantly positive association (p<0.05) with a very strong strength between the WHR index and PCSS (r=0.94).
Conclusion: The WHR index and the PCSS for cellulite have a very strong association.