bluuebunny/crossref_metadata_2025_split huggingface dataset:
Subinertial Response of a Density-Driven Eastern Boundary Poleward Current to Wind Forcing
Authors: Peliz Álvaro , Dubert Jesús , Haidvogel Dale B. 
Published: February 2011

Abstract
A high-resolution primitive equation numerical model is used to generate a poleward flow along a meridionally oriented eastern boundary slope/shelf system by imposing an along-coast density gradient as the forcing mechanism. Wind forcing is applied to the resulting quasi-steady current system, and the subinertial response is analyzed. Parallel experiments with no slope-poleward flow are conducted for comparison. Moderately strong upwelling- and downwelling-favorable, week-to-month-scale wind events modify the poleward flow but do not significantly change the density-driven current structure at the slope. The alongshore transport within the slope region is reduced by 0.2–0.3 Sv (from 1.2 Sv, where Sv ≡ 106 m3 s–1), under the influence of either downwelling or upwelling winds. Independent of the wind direction, the density-driven poleward flow always remains surface intensified. Wind-driven shelf currents develop with a considerable degree of independence from the slope-poleward circulation. On the shelf, the density field is modified by cross-shelf buoyancy advection within the boundary layers and by strong vertical mixing. The presence of the poleward flow over the slope constitutes an important factor in the behavior of the bottom boundary layer at the shelf break and for the patterns of cross-slope circulation.
Kuroshio Extension Variability and Forcing of the Pacific Decadal Oscillations: Responses and Potential Feedback
Authors: Qiu Bo 
Published: February 2011

Abstract
A forcing mechanism is sought for the large-scale circulation changes in the Kuroshio Extension region of the western North Pacific Ocean as inferred by TOPEX/Poseidon sea surface height (SSH) data. The low-frequency signal of the Kuroshio Extension over the last decade was characterized by a modulation in its zonal mean flow intensity: the mean Kuroshio Extension jet weakened progressively from 1993 to 1996 and this trend reversed after 1997. The ability to simulate the major trends in the observed SSH signals with linear vorticity dynamics leads the authors to conclude that the modulation in the zonal mean jet was remotely forced by wind stress curl anomalies in the eastern North Pacific Ocean related to the Pacific decadal oscillations (PDOs). To be specific, the weakening (strengthening) trend in 1993–96 (1997–2001) was caused by westward expansions of negative (positive) SSH anomalies south of the Kuroshio Extension and positive (negative) SSH anomalies north of the Kuroshio Extension. Emergence of oppositely signed SSH anomalies on the two sides of the Kuroshio Extension jet is due to the different propagating speeds of the baroclinic Rossby waves, which carry the wind-induced SSH anomalies generated in the eastern North Pacific at different phases of the PDOs. Hindcasting the Kuroshio Extension jet strength over the last 45 years reveals that the jet modulation has a dominant timescale of ∼12 yr. Given the location of the Kuroshio Extension jet relative to the maximum atmospheric forcing, it is found that this dominant timescale is consistent with the preferred timescale under a stochastic white-noise atmospheric forcing. It is hypothesized that this connection between the Kuroshio Extension strength and the latitudinally dependent baroclinic adjustment contributes to an increase in variance and persistence of the North Pacific midlatitude coupled system on the decadal timescale.
A Novel Imaging Method for Osteochondral Lesions of the Talus—Comparison of SPECT-CT With MRI
Authors: Leumann André , Valderrabano Victor , Plaass Christian , Rasch Helmut , Studler Ueli , Hintermann Beat , Pagenstert Geert I. 
Published: February 2011

Background: Magnetic resonance imaging (MRI) is the current standard in noninvasive diagnostics of osteochondral lesions (OCLs) of the talus. Single-photon emission computed tomography–computed tomography (SPECT-CT) is a new technique that displays different imaging qualities. The influence of the aforementioned diagnostic information on treatment decision making in talar OCLs is not known. Purpose: The aim of the study was to evaluate SPECT-CT in comparison with MRI for image interpretation and decision making in OCLs of the talus. Study Design: Case series; Level of evidence, 4. Methods: Magnetic resonance imaging and SPECT-CT of 25 patients (average age, 32 years; range, 18-69 years) were analyzed by 3 independent orthopaedic surgeons blinded to the study. Raters had to analyze images for predefined criteria of cartilage, subchondral bone plate, and subchondral bone, including bone marrow edema on MRI and scintigraphic activity on SPECT-CT. For MRI alone, SPECT-CT alone, and their combination, the treatment decision had to be defined. Results: In comparison with MRI alone, treatment decision making changed in 48% of the cases with SPECT-CT alone and 52% with SPECT-CT and MRI combined. While cartilage showed good correlation for interpretation between MRI and SPECT-CT, the subchondral bone plate and subchondral bone showed substantial differences. Poor intrarater correlation highlighted the different information provided by the 2 imaging techniques. Poor interrater correlation showed a high heterogeneity in the treatment decision making of OCLs. Conclusion: Compared with MRI, SPECT-CT provides additional information and influences the decision making of OCL treatment. For thorough diagnostic evaluation in OCLs, performing both MRI and SPECT-CT is recommended. Further clinical investigation is needed to see if SPECT-CT in addition to MRI results in improved treatment outcomes.
The Effects of Exhaust Back Pressure on Conventional and Low-Temperature Diesel Combustion
Authors: Cong S , Garner C P , McTaggart-Cowan G P 
Published: February 2011

Modern diesel engines are seeing increasing system and after-treatment complexity which can lead to significant increases in the exhaust back pressure (EBP). This increases the amount of trapped residuals, raising the charge temperature but reducing the oxygen concentration. In this work, these effects of the EBP on diesel engine performance and emissions under conventional and low-temperature diesel combustion (LTC) regimes were investigated. Increasing the EBP resulted in higher pumping work for both combustion modes. While for conventional diesel combustion the effect of the EBP on combustion and emissions were not significant, for LTC the higher back pressures influenced the combustion and emissions formation processes. At low-load conditions, the increase in the charge temperature advanced combustion; at intermediate-load conditions, the reduction in the oxygen concentration delayed it. Smoke emissions were significantly reduced by a higher back pressure at intermediate-load conditions.
On-Board Measurements of Particulate Matter Emissions from a Passenger Train
Authors: Fridell E , Björk A , Ferm M , Ekberg A 
Published: February 2011

A number of measurements of particle emission from railways are presented. The measurements featured a particle instrument mounted in a compartment under a Regina train. The particles were probed in a volume between the coaches. The measurements were done in two campaigns and provide information on how the particle emissions vary with driving patterns. The measurements showed elevated particle concentrations when the train was running. Further, sharp peaks in the particle concentration were observed. It was noted that these peaks coincided with retardation of the train. However, there are also retardations where no peaks were observed. This can be explained from the two parallel breaking systems, one with regenerative breaking and one with mechanical breaks that are present on the Regina train. It is suggest that the observed peaks coincide with the use of the mechanical breaks. It was not possible to fully establish that increased particle concentration coincides with curvature of the rail, even though this was indicated in parts of the data. The size-resolved data showed maxima for the smallest fraction (350 nm) for the number distribution.
Driving and operation strategies for traction-energy saving in mass rapid transit systems
Authors: Malavasi G , Palleschi P , Ricci S 
Published: February 2011

In this article, two models for estimating the energy saving potential on a mass rapid transit system are described. The first model is very useful for analysing the energy and the second one aims at estimating consumptions of a mass rapid transit system in a period of time. The latter was applied first to a generic line with six stations and then to line A of Rome metro network. Results of both applications show that headways of 120–150 s are ideal for energy saving as they allow transfers between braking and accelerating trains.
The effect of contact stress on cartilage friction, deformation and wear
Authors: Lizhang J , Fisher J , Jin Z , Burton A , Williams S 
Published: February 2011

Following hip hemiarthroplasty, a metal femoral head articulates against natural acetabular cartilage. Cartilage friction and wear may be influenced by variables including loading time, contact stress, contact area, sliding distance, and sliding speed. The aim of this study was to investigate the effect of these variables on cartilage friction, deformation and wear in a simulation using idealized geometry model. Bovine cartilage pins were reciprocated against metal plates to mimic a hemiarthroplasty articulation under static loading. The effective coefficient of friction (µeff) under contact stresses (0.5 to 16 MPa), contact areas (12 and 64 mm2), stroke lengths (4 and 8 mm), sliding velocities (4 and 8 mm/s), and loading time (1 and 24 hours) were studied. The permanent deformation of cartilage (after 24 hours of recovery) with and without motion was recorded to assess cartilage linear wear. The µeff was found to remain < 0.35 with contact stresses ≤ 4 MPa. Severe damage to the cartilage occurred at contact stresses > 8 MPa and significantly increased µeff after 12 hours of reciprocation. In long-term, contact area had no significant effect on µeff, and sliding distance and velocity only affected µeff under low contact stresses. The cartilage linear wear increased with contact stress, sliding distance and velocity.
The rate of hemolysis in sickle cell disease correlates with the quantity of active von Willebrand factor in the plasma
Authors: Chen Junmei , Hobbs William E. , Le Jennie , Lenting Peter J. , de Groot Philip G. , López José A. 
Published: February 2011

Abstract
Vaso-occlusion, hemolysis, and oxidative stress are hallmarks of sickle cell disease (SCD). This pathology is accompanied by systemic endothelial activation, rendering the endothelium more adhesive for blood cells, including sickle erythrocytes. Activated endothelial cells display or secrete several adhesive molecules, including von Willebrand factor (VWF). We assessed several VWF parameters in SCD patients at baseline: multimer pattern, antigen concentration (VWF:Ag), activation factor (VWF:AF), and total active VWF (VWF:TA). VWF:AF was determined using a llama nanobody (AU/VWFa-11) that detects a platelet-binding conformation of the A1 domain; VWF:TA was calculated by multiplying VWF:Ag by VWF:AF. SCD plasma contained elevated VWF:Ag and ultralarge VWF multimers. VWF:TA, a measure of total VWF reactivity, correlated closely with hemolysis, as determined by serum lactate dehydrogenase. ADAMTS13 activity and antigen were normal in all patients. These findings suggest an important role for hyperreactive VWF in SCD pathology and connect SCD to other microangiopathies, particularly thrombotic thrombocytopenic purpura.
Is normal hematopoiesis maintained solely by long-term multipotent stem cells?
Authors: Cavazzana-Calvo Marina , Fischer Alain , Bushman Frederic D. , Payen Emmanuel , Hacein-Bey-Abina Salima , Leboulch Philippe 
Published: February 2011

Abstract
The understanding of the hierarchical organization of the human hematopoietic system is of major biologic and clinical significance. The validity of the conventional model in which hematopoiesis is solely maintained by a pool of multipotent long-term hematopoietic stem cells (LT-HSCs) has been recently challenged by several mouse studies. These new data point to the existence of a heterogeneous stem cell population that consists of distinct subsets of LT-HSCs, which include stem cells biased toward lineage-specific differentiation programs. This review attempts to discuss the balanced versus biased patterns of lineage output of human LT-HSCs gathered in 3 different gene therapy trials on the basis of vector integration site analysis by deep sequencing. The distribution of integration sites observed tends to support the validity of the revised model.
The tumor marker Fascin is strongly induced by the Tax oncoprotein of HTLV-1 through NF-κB signals
Authors: Kress Andrea K. , Kalmer Martina , Rowan Aileen G. , Grassmann Ralph , Fleckenstein Bernhard 
Published: February 2011

AbstractOncogenic transformation of CD4+ T cells by human T-cell lymphotropic virus type 1 (HTLV-1) is understood as the initial step to adult T-cell leukemia/lymphoma, a process that is mainly initiated by perturbation of cellular signaling by the viral Tax oncoprotein, a potent transcriptional regulator. In search of novel biomarkers with relevance to oncogenesis, we identified the tumor marker and actin-bundling protein Fascin (FSCN1) to be specifically and strongly up-regulated in both HTLV-1–transformed and adult T-cell leukemia/lymphoma patient-derived CD4+ T cells. Fascin is important for migration and metastasis in various types of cancer. Here we report that a direct link can exist between a single viral oncoprotein and Fascin expression, as the viral oncoprotein Tax was sufficient to induce high levels of Fascin. Nuclear factor-κB signals were important for Tax-mediated transcriptional regulation of Fascin in T cells. This suggests that Fascin up-regulation by Tax contributes to the development of HTLV-1–associated pathogenesis.
Pathogenesis and transmissibility of highly (H7N1) and low (H7N9) pathogenic avian influenza virus infection in red-legged partridge (Alectoris rufa)
Authors: Bertran Kateri , Pérez-Ramírez Elisa , Busquets Núria , Dolz Roser , Ramis Antonio , Darji Ayub , Abad Francesc Xavier , Valle Rosa , Chaves Aida , Vergara-Alert Júlia , Barral Marta , Höfle Ursula , Majó Natàlia 
Published: February 2011

Abstract
An experimental infection with highly pathogenic avian influenza virus (HPAIV) and low pathogenic avian influenza virus (LPAIV) was carried out in red-legged partridges (Alectoris rufa) in order to study clinical signs, gross and microscopic lesions, and viral distribution in tissues and viral shedding. Birds were infected with a HPAIV subtype H7N1 (A/Chicken/Italy/5093/1999) and a LPAIV subtype H7N9 (A/Anas crecca/Spain/1460/2008). Uninoculated birds were included as contacts in both groups. In HPAIV infected birds, the first clinical signs were observed at 3 dpi, and mortality started at 4 dpi, reaching 100% at 8 dpi. The presence of viral antigen in tissues and viral shedding were confirmed by immunohistochemistry and quantitative real time RT-PCR (qRRT-PCR), respectively, in all birds infected with HPAIV. However, neither clinical signs nor histopathological findings were observed in LPAIV infected partridges. In addition, only short-term viral shedding together with seroconversion was detected in some LPAIV inoculated animals. The present study demonstrates that the red-legged partridge is highly susceptible to the H7N1 HPAIV strain, causing severe disease, mortality and abundant viral shedding and thus contributing to the spread of a potential local outbreak of this virus. In contrast, our results concerning H7N9 LPAIV suggest that the red-legged partridge is not a reservoir species for this virus.
Experimental infection of Pacific oyster Crassostrea gigas spat by ostreid herpesvirus 1: demonstration of oyster spat susceptibility
Authors: Schikorski David , Renault Tristan , Saulnier Denis , Faury Nicole , Moreau Pierrick , Pépin Jean-François 
Published: February 2011

Abstract
In 2008 and 2009, acute mortalities occurred in France among Pacific cupped oyster, Crassostrea gigas, spat. Different hypothesis including the implication of environmental factors, toxic algae and/or pathogens have been explored. Diagnostic tests indicated that OsHV-1 including a particular genotype, termed OsHV-1 μVar, was detected in most of samples and especially in moribund oysters with the highlighting of virus particles looking like herpes viruses by TEM examination. In this study, an experimental protocol to reproduce OsHV-1 infection in laboratory conditions was developed. This protocol was based on the intramuscular injection of filtered (0.22 μm) tissue homogenates prepared from naturally OsHV-1 infected spat collected on French coasts during mortality outbreaks in 2008. Results of the experimental trials showed that mortalities were induced after injection. Moreover, filtered tissue homogenates induced mortalities whereas the same tissue homogenates exposed to an ultraviolet (UV) treatment did not induce any mortality suggesting that oyster spat mortalities require the presence of a UV sensitive agent. Furthermore, analysis of injected oyster spat revealed the detection of high amounts of OsHV-1 DNA by real-time quantitative PCR. Finally, TEM analysis demonstrated the presence of herpes virus particles. The developed protocol allowed to maintain sources of infective virus which can be useful for the development of further studies concerning the transmission and the development of OsHV-1 infection.
Reduction in antioxidant enzyme expression and sustained inflammation enhance tissue damage in the subacute phase of spinal cord contusive injury
Authors: Wang Chih-Yen , Chen Jen-Kun , Wu Yi-Ting , Tsai May-Jywan , Shyue Song-Kun , Yang Chung-Shi , Tzeng Shun-Fen 
Published: February 2011

Abstract

Background
Traumatic spinal cord injury (SCI) forms a disadvantageous microenvironment for tissue repair at the lesion site. To consider an appropriate time window for giving a promising therapeutic treatment for subacute and chronic SCI, global changes of proteins in the injured center at the longer survival time points after SCI remains to be elucidated.

Methods
Through two-dimensional electrophoresis (2DE)-based proteome analysis and western blotting, we examined the differential expression of the soluble proteins isolated from the lesion center (LC) at day 1 (acute) and day 14 (subacute) after a severe contusive injury to the thoracic spinal cord at segment 10. In situ apoptotic analysis was used to examine cell apoptosis in injured spinal cord after adenoviral gene transfer of antioxidant enzymes. In addition, administration of chondroitinase ABC (chABC) was performed to analyze hindlimb locomotor recovery in rats with SCI using Basso, Beattie and Bresnahan (BBB) locomotor rating scale.

Results
Our results showed a decline in catalase (CAT) and Mn-superoxide dismutase (MnSOD) found at day 14 after SCI. Accordingly, gene transfer of SOD was introduced in the injured spinal cord and found to attenuate cell apoptosis. Galectin-3, β-actin, actin regulatory protein (CAPG), and F-actin-capping protein subunit β (CAPZB) at day 14 were increased when compared to that detected at day 1 after SCI or in sham-operated control. Indeed, the accumulation of β-actin+ immune cells was observed in the LC at day 14 post SCI, while most of reactive astrocytes were surrounding the lesion center. In addition, chondroitin sulfate proteoglycans (CSPG)-related proteins with 40-kDa was detected in the LC at day 3-14 post SCI. Delayed treatment with chondroitinase ABC (chABC) at day 3 post SCI improved the hindlimb locomotion in SCI rats.

Conclusions
Our findings demonstrate that the differential expression in proteins related to signal transduction, oxidoreduction and stress contribute to extensive inflammation, causing time-dependent spread of tissue damage after severe SCI. The interventions by supplement of anti-oxidant enzymes right after SCI or delayed administration with chABC can facilitate spinal neural cell survival and tissue repair.
Ethanol extract of Scutellaria baicalensis Georgi prevents oxidative damage and neuroinflammation and memorial impairments in artificial senescense mice
Authors: Jeong Kukhuon , Shin Yong-Cheol , Park Sunju , Park Jeong-Su , Kim Namil , Um Jae-Young , Go Hoyeon , Sun Seungho , Lee Sundong , Park Wansu , Choi Youkyung , Song Yunkyung , Kim Gyungjun , Jeon Chanyong , Park Jonghyeong , Lee Keysang , Bang Oksun , Ko Seong-Gyu 
Published: February 2011

Abstract
Aging is a progressive process related to the accumulation of oxidative damage and neuroinflammation. We tried to find the anti-amnesic effect of the Scutellaria baicalens Georgia (SBG) ethanol extract and its major ingredients. The antioxidative effect of SBG on the mice model with memory impairment induced by chronic injection of D-galactose and sodium nitrate was studied. The Y-maze test was used to evaluate the learning and memory function of mice. The activities of superoxide dismutase, catalase and the content of malondialdehyde in brain tissue were used for the antioxidation activities. Neuropathological alteration and expression of bcl-2 protein were investigated in the hippocampus by immunohistochemical staining. ROS, neuroinflammation and apoptosis related molecules expression such as Cox-2, iNOS, procaspase-3, cleaved caspase-3, 8 and 9, bcl-2 and bax protein and the products of iNOS and Cox-2, NO, PGE2, were studied using LPS-activated Raw 264.7 cells and microglia BV2 cells. The cognition of mice was significantly improved by the treatment of baicalein and 50 and 100 mg/kg of SBG in Y-maze test. Both SBG groups showed strong antioxidation, antiinflammation effects with significantly decreased iNOS and Cox-2 expression, NO and PGE2 production, increased bcl-2 and decreased bax and cleaved caspase-3 protein expression in LPS induced Raw 264.7 and BV2 cells. We also found that apoptotic pathway was caused by the intrinsic mitochondrial pathway with the decreased cleaved caspase-9 and unchanged cleaved caspase-8 expression. These findings suggest that SBG, especially high dose, 100 mg/kg, improved the memory impairments significantly and showed antioxidation, antiinflammation and intrinsic caspase-mediated apoptosis effects.