bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive transcriptome, miRNA and kinome profiling and its clinical translation for personalized lung cancer therapy
Authors: Zhong Shen , Börgeling Yvonne , Zardo Patrick , Jonigk Danny , Borlak Jürgen 
Published: May 2024

Abstract

Background: Lung cancer (LC) is the second most frequently diagnosed cancer worldwide and basic research identified oncogenic driver mutations which sparked the development of kinase-inhibitor (KI) based therapies. However, less than10% of LC patients carry driver mutations, and the majority of patients are not recommended for first-line KI-treatment. Importantly, sustained proliferative signalling is common to all cancers. Therefore, we searched for signalling networks across different histological types of LC, and performed comprehensive transcriptome, miRNA and kinome profiling to detect aberrant kinases for targeted therapies based on a patient’s individual needs.
Methods: We performed molecular pathology, transcriptomics and miRNA profiling across ninety-five well-characterised LC patients and validated results by considering cross-linking immunoprecipitation-sequencing data. We independently confirmed the results by evaluating the TCGA data of N=524 adeno- and 497 squamous cell carcinomas. We used the PamGene platform to investigate kinome profiles, and established significance of KI by evaluating published siRNA and CRISPR-mediated mRNA knockdown and cell viability data in human LC cell lines.
Results: We identified 439, 1240, 383 and 320 significantly upregulated genes for adeno, squamous, neuroendocrine and metastatic cases, and there are 1092, 1477, 609 and 1267 downregulated DEGs. Based on gene enrichment analysis and validated miRNA-gene interactions, we constructed networks specific for adeno-, squamous, neuroendocrine and metastatic LC. Additionally, molecular profiling revealed 137 significantly upregulated kinases (range 2 to 26-fold) of which 65 and 72, respectively are tyrosine and serine-threonine kinases while 6 kinases carry driver mutations. Irrespective of the histological type of LC there are 21 kinases commonly upregulated. Bioinformatics decoded tumour specific miRNA-gene networks, and we identified kinases which function as master regulators at the apex of signalling networks. Typically, the networks consisted of 14, 9, 16 and 19 highly regulated kinases in adeno-, squamous, neuroendocrine and metastatic LC, and by considering regulatory networks, rational selection of KI-based therapies in patients with acquired drug resistance becomes feasible. Finally, gene knock-down studies confirmed inhibition of cell viability in a panel of human lung cancer cell lines.
Conclusions: Our study broadens the perspective of precision oncology in LC, and we propose a framework to bypass acquired drug resistance.
Impact of a multi-level, multi-component intervention to improve elementary school physical education on student cardiorespiratory fitness: an application of the parametric g-formula
Authors: Thompson Hannah R. , Madsen Kristine A. , Nguyen Caroline , McKenzie Thomas L. , Picciotto Sally 
Published: May 2024

Abstract

Background: School physical education is an important population-level health intervention for improving youth fitness. The purpose of this study is to determine the causal impact of New York City’s PE Works program on student cardiorespiratory fitness.
Methods: This longitudinal study (2014-2019) includes 581 elementary schools (n=315,999 4th/5th-grade students; 84% non-white; 74% who qualify for free or reduced-price meals). We apply the parametric g-formula to address schools’ time-varying exposure to intervention components and time-varying confounding.
Results: After four years of staggered PE Works implementation, 49.7% of students per school (95% CI: 42.6%, 54.2%) met age/sex-specific cardiorespiratory fitness standards. Had PE Works not been implemented, we estimate 45.7% (95% CI: 36.9%, 52.1%) would have met fitness standards. Had PE Works been fully implemented in all schools from the program’s inception, we estimate 57.4% (95% CI: 49.1%, 63.3%) would have met fitness standards. Adding a PE teacher, alone, had the largest impact (6.4% (95% CI: 1.0, 12.0) increase).
Conclusion: PE Works, which included providing PE teachers, training for classroom teachers, and administrative/teacher support for PE, positively impacted student cardiorespiratory health. Mandating and funding multilevel, multicomponent PE programs is an important public health intervention to increase children’s cardiorespiratory fitness.
Development and application of an immuoperoxidase monolayer assay for the detection of PRRSV
Authors: LI peng , GUAN Chunxiao , WANG Liping , WANG Huajian , SUN Guopeng , HE Jinjiao , LIU Xingyou , WANG Xuannian 
Published: May 2024

Abstract

Background
Porcine reproductive and respiratory syndrome (PRRS), caused by porcine reproductive and respiratory syndrome virus (PRRSV), is a highly contagious disease with high morbidity and mortality that affects the global swine industry. So far, there is still a widespread dissemination of PRRSV with obvious genetic variations in swine population, resulting in huge economic losses annually. Therefore, accurate laboratory diagnosis is needed to quickly confirm PRRSV infection.
Results
An immunoperoxidase monolayer assay (IPMA) was developed for the specific and sensitive detection of PRRSV based on a broad-spectrum anti-PRRSV monoclonal antibody (mAb) 28F6. The mAb 28F6-based IPMA could specifically detect PRRSV and possessed no cross-reactions with CSFV, PCV2, and PEDV. Sensitivity analysis showed that the limit of detection of the IPMA reached 10− 2.25 TCID50/100 µL. There was no significant difference in the detection of PRRSV of different passages with different batches of mAb 28F6, indicating that the IPMA had good repeatability. In addition, the IPMA could recognize a number of PRRSV variants including field strains such as BJ-4, HN07-1, and NADC30-like strain, as well as vaccine strains like HuN4-F112, JXA1-R, TJM-F92, GDr180, VR2332, CH-1R, and R98. Validation of the IPMA showed that it was in 100% consistency with qRT-PCR on the detection of 108 clinical samples.
Conclusions
The IPMA could meet the demand for the specific and sensitive detection of PRRSV, which is helpful for accurate monitoring and early warning of PRRSV infections.
Construction of a comprehensive management plan for patients undergoing radical prostatectomy based on the pender model: A delphi method study
Authors: Yu Chen , Fang Guo , Luqiang Zhou , Siyue Li , Xingliang Yang , Ji Zheng 
Published: May 2024

Abstract

Purpose
Constructing a comprehensive management plan for prostate cancer surgery patients based on the Pender health promotion model, to guide clinical nurses in implementing rehabilitation, education, and follow-up work for prostate cancer surgery patients, and to provide a basis for formulating prostate cancer nursing guidelines.
Methods
On the basis of literature research and expert group meetings, a comprehensive management draft for prostate cancer surgery patients based on the Pender model is constructed. Use the Delphi method for two rounds of expert inquiry, and modify the entries according to the expert inquiry opinions.
Results
In the first round of inquiry, 21 experts were consulted. The effective recovery rate was 95.23%; In the second round, 20 experts were consulted, and the effective recovery rate was 100%. The expert authority coefficient is 0.88, the coefficient of variation for each item in the second round is 0-0.19, the Kendall harmony coefficient is 0.197 (p < 0.001), and the mean importance assignment is 3.80-4.95. Finally, a comprehensive management plan for prostate cancer surgery patients based on the Pender model was formed, including 4 first-level indicators, 15 second-level indicators, and 42 third-level indicators.
Conclusions
The comprehensive management plan for prostate cancer surgery patients based on the Pender model developed through research is scientific and reliable, which can provide reference for nursing research and practice of prostate cancer surgery patients.
A Novel Index Weight Determination Method for Improving Objectivity
Authors: Ma Zifei , Li Qinghua , Li Wengang , He Yun , Yang Juan , Li Yang , Li Jing , Jiang Rong 
Published: May 2024

Abstract

The index weight determines the scientificity and rationality of the comprehensive evaluation results. However, the traditional index calculation method, which is scored directly by decision-makers, excessively depends on the degree of experience and knowledge accumulation of decision-makers. Different decision-makers’ understandings of the index have great differences and fuzziness, which makes the weight calculation results biased or even wrong due to the influence of human subjective factors. To solve this problem, this paper considers that the index weight value contains the decision-makers’ personal subjective information and common objective information, and then proposes a weight calculation approach to improve the objectivity of the index based on the Superiority of Neighboring Objectives and the Ensemble Empirical Mode Decomposition, which can eliminate the subjective information in the weight value and obtain a more objective weight value. Finally, the rationality and objectivity of the proposed method are verified by comparing them with the classical AHP, subjective weighting method and objective weighting method.
Investigating the association between cataract patients and the risk of herpes zoster in a cohort study
Authors: Lai Shih-Wei , Kuo Yu-Hung , Liao Kuan-Fu 
Published: May 2024

Abstract

Objective
This cohort study aimed to elucidate whether cataract patients are at an increased risk of herpes zoster in Taiwan.
Methods
A cohort of cataract patients, aged 20–84 years, was assembled from electronic health records of Taiwan National Health Insurance Program spanning a period of eight years (2013–2020). A non-cataract group matched for sex, age, and comorbidities was also identified from the same database. The incidence rate of herpes zoster within the follow-up period was calculated between the two groups. The risk of herpes zoster was compared between the two groups using a Cox proportional hazards model, adjusting for potential confounders.
Results
The study included 1,299,685 individuals in the cataract group. The mean age was 64 years old and about 43.9% of study subjects were male. The non-cataract group had similar numbers of study subjects and comparable distributions in terms of sex and age. The incidence rate of herpes zoster was 10.84 per 1000 person-years in the cataract group and 6.36 per 1000 person-years in the non-cataract group (incidence rate ratio = 1.71, 95% CI = 1.69–1.72). After adjusting for potential confounders, the hazard ratio was 1.71 for herpes zoster in individuals with cataract when compared to those without cataract (95%CI = 1.69–1.73).
Conclusion
Our cohort study reveals that cataract patients are at an increased risk of developing herpes zoster. Our findings highlight the importance of considering the increased susceptibility to herpes zoster in cataract patients and the potential benefits of preventive measures such as vaccination.
Effect of 18F-FDG combined with 18F-AlF-NOTA-octreotide PET/CT imaging on the grading of neuroendocrine tumors.
Authors: Dong Qing , Xu Wengui , Zhu Lei , Liu Yang , Wang Jian , Liu Bingwang , Chen Kun , Wan Xing 
Published: May 2024

Abstract

Neuroendocrine tumors (NENs) are tumors derived from neuroendocrine cells and are commonly found in the pancreas, lungs, gastrointestinal tract, and other tissues. Traditional imaging methods such as CT and MRI have certain limitations in the diagnosis of NENs, and can not provide information about the metabolic activity of tissues. Molecular imaging techniques, particularly positron emission tomography/computed tomography (PET/CT), are able to provide biological information about tumor metabolism, providing more comprehensive information for the diagnosis and treatment of NENs.
Objective
The aim of this study was to compare and evaluate the diagnostic performance of 18F-AlF-octreotide and 18F-FDG PET/CT in patients with neuroendocrine tumors (NENs) and the effect of different tumor grades on imaging agent uptake.
Methods
We retrospectively analyzed the clinical data of 19 patients with NENs, in which 18F-AlF-octreotide and 18F-FDG PET/CT were used for imaging. The performance of the two imaging agents was evaluated by extracting or calculating the corresponding metabolic parameters and stratifying the patients with different grades.
Results
There were significant differences in metabolic parameters such as TSR, SUVmax, SUVmean and SULmax between the two imaging agents (P < 0.05), but the differences in other indicators were not statistically significant. For patients with different grades of NENs, 18F-AlF-octreotide showed higher sensitivity in G1 and G2 grades, while 18F-FDG showed better performance in G3 grades.
Conclusion
The results of this study indicate that 18F-AlF-octreotide and 18F-FDG PET/CT have different diagnostic manifestations according to different grades of NENs patients. In clinical practice, the heterogeneity of patients should be considered comprehensively, and appropriate imaging agents should be selected for diagnosis to improve the accuracy of NENs and the guidance of personalized treatment.
A Review Report on Real Time Health Condition Monitoring of Devices in Solar Power Transmission Systems
Authors: Kambhampati Venkata Govardhan Rao , Thalanki Venkata Sai Kalyani , Nittala Ramchandra 
Published: May 2024

Abstract

As renewable energy generation grows globally, real-time asset management is crucial, particularly for offshore and remote systems. Electric grids are rapidly adopting renewable energy generation. Currently, there is no cost-effective condition monitoring method for real-time assessment of renewable energy sources, enabling intelligent asset management decisions to optimise utilisation and prevent unforeseen problems. Transformers are key assets that connect renewable generation plants to the grid. If one fails, generation can be lost for a lengthy time. The transformer in an on-grid system steps up or down voltage as needed. Transformer monitoring is crucial to Smart Grids. Transformer monitoring also allows demand analysis—what each family, business, or commercial institution consumes, where it consumes more, and when demand peaks. By its nature most renewable generation is intermittent, which places increased stress on transformers. To achieve an efficient, better and reliable generation of solar energy, it is necessary to monitor the transformers and solar generation system continuously. Hence in this work, real time health condition monitoring of devices in solar power transmission systems is presented. This system monitors the devices in real time with the help of IoT. Understanding transformer health and performance with real-time monitoring allows developing and optimizing proactive maintenance strategies for improving the performance of solar power transmission systems.
Medial cortical bone thickness of the tibial diaphysis in osteoarthritic knees is related to lower extremity alignment and tibial morphology
Authors: Maeda Keisuke , Mochizuki Tomoharu , Tanifuji Osamu , Katsumi Ryota , Kobayashi Koichi , Kawashima Hiroyuki 
Published: May 2024

Abstract

Background
The purpose of this study was to clarify (1) the differences in cortical bone thickness (CBT) of the tibial diaphysis between healthy and osteoarthritic knees and (2) the influences of the femorotibial angle (FTA) and inclination of the medial compartment of the proximal tibia (MCT) on tibial CBT.
Methods
The study assessed 60 subjects with varus knee osteoarthritis (OA) (22 males and 38 females; mean age, 74 ± 7 years) and 53 healthy elderly subjects (28 males and 25 females; mean age, 70 ± 6 years). Three-dimensional estimated CBT of the tibial diaphysis was automatically calculated for 2752–11296 points using high-resolution measurements from CT. The standardized CBT was assessed in 24 regions by combining six heights and four areas. Additionally, the association between the CBT, each FTA, and MCT inclination was investigated.
Results
The OA group showed a thicker CBT in the medial areas than in the lateral areas of the proximal tibia, while the healthy group had a thicker lateral CBT. The medial-to-lateral ratio of the proximal tibia was significantly higher in the OA group than in the healthy group. The proximal-medial CBT correlated with FTA and MCT inclinations in the OA group.
Conclusions
This study demonstrated that varus osteoarthritic knees showed a different trend of proximal-medial CBT with associations in FTA and MCT inclination from healthy knees, possibly due to medial load concentration.
Association Between Sleep Duration and Osteoporosis in Older Adults: A Cross-Sectional Study in Taiwan
Authors: Hong-Jhe Chen , Kuo Yu-En , Kuo Terry B.J. , Yang Cheryl C.H. , Ou Hsun , Chen Tien-Yu 
Published: May 2024

Abstract

Objectives:
This study explored the association between sleep duration and osteoporosis risk in older adults aged > 65 years and investigated the influence of sex, heart rate variability, and sleep quality.
Methods:
In this cross-sectional study, osteosarcopenia screening was conducted among older adults in long-term care centers operated by Kaohsiung Veterans General Hospital in Taiwan in 2019. Osteosarcopenia was diagnosed using dual-energy X-ray absorptiometry. Questionnaires explored the relationship between sleep duration and bone mineral density. Initially, data from 357 participants were collected, with missing data and participants under 65 years of age excluded, resulting in 326 participants for the final analysis.
Results:
This study involved 326 participants with a mean age > 80 years. Sleep duration < 4 h was significantly associated with an increased risk of osteoporosis (odds ratio: 2.30, 95% confidence interval: 1.15–4.57) regardless of sex. Additionally, heart rate variability (HRV) was not significantly associated with sleep quality or osteoporosis risk.
Conclusions:
Among older adults aged > 80 years, sleep duration < 4 h is associated with an increased risk of osteoporosis. HRV may not be an optimal indicator of sleep quality in this demographic.
The bidirectional prediction between chronotype and depression and its mediating model in a longitudinal study among adolescents
Authors: Cao Rui , Jia Yihui , Wang Yefei 
Published: May 2024

Abstract

Although many studies have confirmed the close association between chronotype and depression, the prediction between chronotype and depression, as well as the mechanisms of their influence are rarely investigated in adolescents. This study aimed to confirm the bidirectional prediction between chronotype and depression, and to exam the mediating effects of cognitive reappraisal, expressive suppression, and rumination on the association between chronotype and depression in adolescents. This was a longitudinal study over a 5-month period with 2 surveys (N = 634; 41.32% male, 58.68% female). Pearson correlation analysis was utilized to examine the relationship among all variables. Hierarchical regression analysis was conducted utilized to analyze the relationship between chronotype and depression. Conduct mediation analysis to exam the mediating roles of cognitive reappraisal, expressive suppression, and rumination on the association between chronotype and depression. Results showed that after controlling for gender, age (T1), age (T2), and depression (T1), chronotype at baseline negatively predicted depression 5 months later. After controlling for gender, age (T1), age (T2), and chronotype (T1), depression at baseline negatively predicted chronotype 5 months later. Chronotype indirectly predicted depression though cognitive reappraisal, expressive suppression, and rumination, but depression indirectly predicted chronotype though cognitive reappraisal, and rumination. This study found a bidirectional prediction between chronotype and depression, andthe underlying mechanism of the chronotype predicting depression and the underlying mechanism of depression predicting chronotype were different.
Deep-eutectic-solvent-based ultrasound-assisted extraction of polysaccharides from maca: Optimization using Taguchi methodology and comparison with the conventional method
Authors: Kim Eun Jeong , Kim Choon Young , Yoon Kyung Young 
Published: May 2024

Abstract

Deep eutectic solvent (DES) was used for the ultrasound-assisted extraction (UAE) of polysaccharides from maca. Extraction parameters affecting the extraction yield were experimentally identified and their significance was further investigated using the Taguchi method. DES prepared from choline chloride and urea afforded the highest yield (20.03%) and was chosen as the solvent for UAE. The optimal extraction parameters were: water content of 30% for DES, ultrasonic power of 300 W, and extraction time of 20. The extraction yield (26.28%) of maca polysaccharides (MPs) obtained using these extraction parameters was more than twice that of MPs obtained by hot-water extraction and UAE with water. Moreover, MPs obtained through DES-based extraction exhibited various biological functions such as inhibiting pancreatic α-amylase and α-glucosidase activities, delaying absorption of glucose and bile acid, and stimulating the probiotic. Therefore, DES can be used to extract polysaccharides from maca with biological action as a highly efficient and non-polluting alternative solvent.
Characteristics of Injuries Among Elite Badminton Players: A Systematic Review
Authors: Shaharudin Muhammad Iqbal , Muhamed Ahmad Munir Che , YUSOF Hazwani AHMAD 
Published: May 2024

Abstract

Background
Badminton is a fast-paced sport that has captivated more than 700 million people globally. Competing in badminton requires a high level of skill and strategic thinking, encompassing attributes such as agility, quick reaction times, endurance, spatial awareness, and strength. Despite its non-contact nature, badminton frequently results in injuries, encompassing both overuse injuries and acute traumatic incidents. The game requires a high level of physical fitness and involves intricate movements that place excessive strain on the upper extremities, lower extremities, and trunk region. This review aimed to identify the characteristics of musculoskeletal injuries in elite badminton players.
Methods
A systematic search through the electronic bibliographic databases, Web of Science (WoS), Scopus, ClinicalKey, Cochrane Library, and Google Scholar in the past ten years between 2013 and 2023. Studies reported on elite badminton players, and the characteristics of injuries were included. The study's development followed the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines. It was registered in the PROSPERO (International Prospective Register of Systematic Reviews). Quality assessment was performed using the Newcastle Ottawa Scale (NOS) and Critical Appraisal Skills Programme (CASP).
Results
The study included 11 eligible articles. The result indicated that more than 40% of badminton players reported having injuries. More cases of lower extremity injury were reported, followed by upper and trunk regions. Tendinopathy, sprain, and strain are the most common injuries sustained by badminton players.
Conclusions
Early identification of athletes susceptible to injury risk is key to formulating a successful injury prevention initiative. Identifying the characteristics of injuries can offer valuable evidence for healthcare professionals, sports institutes, and coaches to implement injury prevention strategies for athletes.