bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mental health care for irregular migrants in Europe: Barriers and how they are overcome
Authors: Straßmayr Christa , Matanov Aleksandra , Priebe Stefan , Barros Henrique , Canavan Reamonn , Díaz-Olalla José Manuel , Gabor Edina , Gaddini Andrea , Greacen Tim , Holcnerová Petra , Kluge Ulrike , Welbel Marta , Nicaise Pablo , Schene Aart H , Soares Joaquim JF , Katschnig Heinz 
Published: May 2012

Abstract

Background
Irregular migrants (IMs) are exposed to a wide range of risk factors for developing mental health problems. However, little is known about whether and how they receive mental health care across European countries. The aims of this study were (1) to identify barriers to mental health care for IMs, and (2) to explore ways by which these barriers are overcome in practice.

Methods
Data from semi-structured interviews with 25 experts in the field of mental health care for IMs in the capital cities of 14 European countries were analysed using thematic analysis.

Results
Experts reported a range of barriers to mental health care for IMs. These include the absence of legal entitlements to health care in some countries or a lack of awareness of such entitlements, administrative obstacles, a shortage of culturally sensitive care, the complexity of the social needs of IMs, and their fear of being reported and deported. These barriers can be partly overcome by networks of committed professionals and supportive services. NGOs have become important initial points of contact for IMs, providing mental health care themselves or referring IMs to other suitable services. However, these services are often confronted with the ethical dilemma of either acting according to the legislation and institutional rules or providing care for humanitarian reasons, which involves the risk of acting illegally and providing care without authorisation.

Conclusions
Even in countries where access to health care is legally possible for IMs, various other barriers remain. Some of these are common to all migrants, whilst others are specific for IMs. Attempts at improving mental health care for IMs should consider barriers beyond legal entitlement, including communicating information about entitlement to mental health care professionals and patients, providing culturally sensitive care and ensuring sufficient resources.
Formation of Se (0) Nanoparticles by Duganella sp. and Agrobacterium sp. isolated from Se-laden soil of North-East Punjab, India
Authors: Bajaj Mini , Schmidt Susan , Winter Josef 
Published: May 2012

Abstract

Background
Selenium (Se) is an essential trace element, but is toxic at high concentrations. Depending upon the geological background, the land use or on anthropogenic pollution, different amounts of Se may be present in soil. Its toxicity is related to the oxyanions selenate and selenite as they are water soluble and bioavailable. Microorganisms play an important role in Se transformations in soil and its cycling in the environment by transforming water-soluble oxyanions into water insoluble, non-toxic elemental Se (0). For this study, soil samples were collected from selenium-contaminated agricultural soils of Punjab/India to enrich and isolate microbes that interacted with the Se cycle.

Results
A mixed microbial culture enriched from the arable soil of Punjab could reduce 230 mg/l of water soluble selenite to spherical Se (0) nanoparticles during aerobic growth as confirmed by SEM-EDX. Four pure cultures (C 1, C 4, C 6, C 7) of Gram negative, oxidase and catalase positive, aerobic bacteria were isolated from this mixed microbial consortium and identified by 16 S rDNA gene sequence alignment as two strains of Duganella sp. (C 1, C 4) and two strains of Agrobacterium sp.(C 6, C 7). SEM/TEM-EDX analyses of the culture broth of the four strains revealed excretion of uniformly round sharply contoured Se (0) nanoparticles by all cultures. Their size ranged from 140–200 nm in cultures of strains C 1 and C 4, and from 185–190 nm in cultures of strains C 6 and C 7. Both Duganella sp. revealed better selenite reduction efficiencies than the two Agrobacterium sp.

Conclusions
This is the first study reporting the capability of newly isolated, aerobically growing Duganella sp. and Agrobacterium sp. from soils of Punjab/India to form spherical, regularly formed Se (0) nanoparticles from water soluble selenite. Among others, the four strains may significantly contribute to the biogeochemical cycling of Se in soil. Bioconversion of toxic selenite to non-toxic Se (0) nanoparticles under aerobic conditions in general may be useful for detoxification of agricultural soil, since elemental Se may not be taken up by the roots of plants and thus allow non-dangerous fodder and food production on Se-containing soil.
Encapsulation of Alpha-1 antitrypsin in PLGA nanoparticles: In Vitro characterization as an effective aerosol formulation in pulmonary diseases
Authors: Pirooznia Nazanin , Hasannia Sadegh , Lotfi Abbas Sahebghadam , Ghanei Mostafa 
Published: May 2012

Abstract

Background
Alpha 1- antitrypsin (α1AT) belongs to the superfamily of serpins and inhibits different proteases. α1AT protects the lung from cellular inflammatory enzymes. In the absence of α1AT, the degradation of lung tissue results to pulmonary complications. The pulmonary route is a potent noninvasive route for systemic and local delivery. The aerosolized α1AT not only affects locally its main site of action but also avoids remaining in circulation for a long period of time in peripheral blood. Poly (D, L lactide-co glycolide) (PLGA) is a biodegradable and biocompatible polymer approved for sustained controlled release of peptides and proteins. The aim of this work was to prepare a wide range of particle size as a carrier of protein-loaded nanoparticles to deposit in different parts of the respiratory system especially in the deep lung. Various lactide to glycolide ratio of the copolymer was used to obtain different release profile of the drug which covers extended and rapid drug release in one formulation.

Results
Nonaqueous and double emulsion techniques were applied for the synthesis of nanoparticles. Nanoparticles were characterized in terms of surface morphology, size distribution, powder X-ray diffraction (XRD), encapsulation efficiency, in vitro drug release, FTIR spectroscopy and differential scanning calorimetry (DSC). To evaluate the nanoparticles cytotoxicity, cell cytotoxicity test was carried out on the Cor L105 human epithelial lung cancer cell line.
Nanoparticles were spherical with an average size in the range of 100 nm to 1μ. The encapsulation efficiency was found to be higher when the double emulsion technique was applied. XRD and DSC results indicated that α1AT encapsulated in the nanoparticles existed in an amorphous or disordered-crystalline status in the polymer matrix. The lactic acid to glycolic acid ratio affects the release profile of α1AT. Hence, PLGA with a 50:50 ratios exhibited the ability to release %60 of the drug within 8, but the polymer with a ratio of 75:25 had a continuous and longer release profile. Cytotoxicity studies showed that nanoparticles do not affect cell growth and were not toxic to cells.

Conclusion
In summary, α1AT-loaded nanoparticles may be considered as a novel formulation for efficient treatment of many pulmonary diseases.
Clinical, haematological and biochemical responses of sheep undergoing autologous blood transfusion
Authors: Sousa Rejane Santos , Chaves Dowglish Ferreira , Barrêto-Júnior Raimundo Alves , Sousa Isadora Karolina Freitas , Soares Herbert Sousa , Barros Isabella Oliveira , Minervino Antonio Humberto Hamad , Ortolani Enrico Lippi 
Published: May 2012

Abstract

Background
This study aimed to evaluate the clinical, haematological and biochemical responses to autologous blood transfusion and the feasibility of this practice in sheep. Thus, we used eight male, 8 months old sheep, weighing on average 30 kg, from which 15 mL/kg of whole blood was collected and stored in CPDA-1 bags. Blood samples were refrigerated for 8 days and subsequently re-infused. The clinical, haematological and biochemical parameters were evaluated before blood collection and reinfusion, after 10 minutes of collection and reinfusion, after 3, 6, 12, 24, 48, 96 and 192 hours after collection and reinfusion.

Results
With respect to clinical parameters, we observed a decrease in heart rate after 24, 48 and 196 hours from reinfusion compared to basal values (p < 0.05). Haematological variables including globular volume and erythrocyte counts showed a significant decrease (p < 0.01) at all time points after collection and increased (p < 0.01) at all time points after reinfusion. There was a significant increase in total protein and calcium at all time points after reinfusion (p < 0.05).

Conclusion
Autologous transfusion in sheep slightly altered the physiological, biochemical and haematological responses of sheep, indicating that the technique proposed is safe and can be applied in the clinical practice of this species. The 8 d period was not sufficient for complete recovery of the haematological parameters after blood collection.
Modeling of human factor Va inactivation by activated protein C
Authors: Bravo Maria Cristina , Orfeo Thomas , Mann Kenneth G , Everse Stephen J 
Published: May 2012

Abstract

Background
Because understanding of the inventory, connectivity and dynamics of the components characterizing the process of coagulation is relatively mature, it has become an attractive target for physiochemical modeling. Such models can potentially improve the design of therapeutics. The prothrombinase complex (composed of the protease factor (F)Xa and its cofactor FVa) plays a central role in this network as the main producer of thrombin, which catalyses both the activation of platelets and the conversion of fibrinogen to fibrin, the main substances of a clot. A key negative feedback loop that prevents clot propagation beyond the site of injury is the thrombin-dependent generation of activated protein C (APC), an enzyme that inactivates FVa, thus neutralizing the prothrombinase complex. APC inactivation of FVa is complex, involving the production of partially active intermediates and “protection” of FVa from APC by both FXa and prothrombin. An empirically validated mathematical model of this process would be useful in advancing the predictive capacity of comprehensive models of coagulation.

Results
A model of human APC inactivation of prothrombinase was constructed in a stepwise fashion by analyzing time courses of FVa inactivation in empirical reaction systems with increasing number of interacting components and generating corresponding model constructs of each reaction system. Reaction mechanisms, rate constants and equilibrium constants informing these model constructs were initially derived from various research groups reporting on APC inactivation of FVa in isolation, or in the presence of FXa or prothrombin. Model predictions were assessed against empirical data measuring the appearance and disappearance of multiple FVa degradation intermediates as well as prothrombinase activity changes, with plasma proteins derived from multiple preparations. Our work integrates previously published findings and through the cooperative analysis of in vitro experiments and mathematical constructs we are able to produce a final validated model that includes 24 chemical reactions and interactions with 14 unique rate constants which describe the flux in concentrations of 24 species.

Conclusion
This study highlights the complexity of the inactivation process and provides a module of equations describing the Protein C pathway that can be integrated into existing comprehensive mathematical models describing tissue factor initiated coagulation.
Trapping of Rift Valley Fever (RVF) vectors using Light Emitting Diode (LED) CDC traps in two arboviral disease hot spots in Kenya
Authors: Tchouassi David P , Sang Rosemary , Sole Catherine L , Bastos Armanda DS , Cohnstaedt Lee W , Torto Baldwyn 
Published: May 2012

Abstract

Background
Mosquitoes’ response to artificial lights including color has been exploited in trap designs for improved sampling of mosquito vectors. Earlier studies suggest that mosquitoes are attracted to specific wavelengths of light and thus the need to refine techniques to increase mosquito captures following the development of super-bright light-emitting diodes (LEDs) which emit narrow wavelengths of light or very specific colors. Therefore, we investigated if LEDs can be effective substitutes for incandescent lamps used in CDC light traps for mosquito surveillance, and if so, determine the best color for attraction of important Rift Valley Fever (RFV) vectors.

Methods
The efficiency of selected colored LED CDC light traps (red, green, blue, violet, combination of blue-green-red (BGR)) to sample RVF vectors was evaluated relative to incandescent light (as control) in a CDC light trap in two RVF hotspots (Marigat and Ijara districts) in Kenya. In field experiments, traps were baited with dry ice and captures evaluated for Aedes tricholabis, Ae. mcintoshi, Ae. ochraceus, Mansonia uniformis, Mn. africana and Culex pipiens, following Latin square design with days as replicates. Daily mosquito counts per treatment were analyzed using a generalized linear model with Negative Binomial error structure and log link using R. The incidence rate ratios (IRR) that mosquito species chose other treatments instead of the control, were estimated.

Results
Seasonal preference of Ae.mcintoshi and Ae. ochraceus at Ijara was evident with a bias towards BGR and blue traps respectively in one trapping period but this pattern waned during another period at same site with significantly low numbers recorded in all colored traps except blue relative to the control. Overall results showed that higher captures of all species were recorded in control traps compared to the other LED traps (IRR < 1) although only significantly different from red and violet.

Conclusion
Based on our trapping design and color, none of the LEDs outcompeted the standard incandescent light. The data however provides preliminary evidence that a preference might exist for some of these mosquito species based on observed differential attraction to these light colors requiring future studies to compare reflected versus transmitted light and the incorporation of colored light of varying intensities.
Population genetic structure of Ascaridia galli re-emerging in non-caged laying hens
Authors: Höglund Johan , Morrison David A , Engström Annie , Nejsum Peter , Jansson Désirée S 
Published: May 2012

Abstract

Background
The poultry roundworm Ascaridia galli has reappeared in hens kept for egg production in Sweden after having been almost absent a decade ago. Today this is a frequent intestinal nematode parasite in non-caged laying hens. The aim of this study was to investigate the genetic diversity (Fst) in A. galli collected from different poultry production sites in southern Sweden, to identify possible common routes of colonization.

Methods
Adult parasites (n = 153) from 10 farms, including both broiler breeder parents and laying hens, were investigated by amplified restriction fragment length polymorphism analysis (AFLP). Worms from a Danish laying hen farm were also included for comparison. Most of the farms were represented by worms from a single host, but on two farms multiple samples from different hosts were assessed in order to study flock variation.

Results
A total of 97 fragments (loci) were amplified among which 81% were variable alleles. The average genetic diversity was 0.13 (range = 0.09-0.38), which is comparable to other AFLP studies on nematodes of human and veterinary importance. Within-farm variation showed that worms harboured by a single hen in a flock covered most of the A. galli genetic variation within the same flock (Fst = 0.01 and 0.03 for two farms). Between-farm analysis showed a moderate population genetic structure (Fst = 0.13), along with a low mutational rate but high gene flow between different farms, and absence of strong genetic selection. Network analysis showed repeated genetic patterns among the farms, with most worms on each farm clustering together as supported by high re-allocation rates.

Conclusions
The investigated A. galli populations were not strongly differentiated, indicating that they have undergone a genetic bottlenecking and subsequent drift. This supports the view that the investigated farms have been recently colonized, and that new flocks are reinfected upon arrival with a stationary infection.
Asian Boundaries, Documentary Regimes, and the Political Economy of the Personal
Authors: Chen Tina Mai 
Published: May 2012

This article inquires into how “the personal” assumes specific positions relative to truth claims constitutive of twentieth-century national and international documentary systems. It analyzes migration of those identified as Chinese in and out of Burma during the late 1930s and 1940s. The focus is on how individuals, networks of people, and institutions presented and relied on information about families, physical appearance, personal interviews, and affective ties to make sense of migratory patterns resulting from Japanese military expansion.
The article argues that by the mid-1940s a layered inscription of citizenship, loyalty, and labor via “the personal” formed the basic structure of meaning mobilized in identification documents. It traces the specific ways in which this emerged around practices and policies developed to monitor and control Chinese migration within the Burma-China-India nexus. These structures not only prescribed particular evidentiary standards regarding personal interactions and signifiers that became part of national and international norms, but they also created hierarchies of affect and emotional attachment deemed appropriate for particular groupings of people, spatial designations, and material exchanges.
The focus on the personal is part of an effort to continue interrogation of Asia and its constitution as object of knowledge and lived experience in the past, present, and future. This article attends to the “the personal” as a site of potentiality to be analyzed historically as well as opened up through a continuous rethinking of Asia and the racialized relationship among nationality, state, and international orders.
Restructured endoplasmic reticulum generated by mutant amyotrophic lateral sclerosis-linked VAPB is cleared by the proteasome
Authors: Papiani Giulia , Ruggiano Annamaria , Fossati Matteo , Raimondi Andrea , Bertoni Giovanni , Francolini Maura , Benfante Roberta , Navone Francesca , Borgese Nica 
Published: May 2012

Summary
VAPB (vesicle-associated membrane protein-associated protein B) is a ubiquitously expressed, ER-resident tail-anchored protein that functions as adaptor for lipid-exchange proteins. Its mutant form, P56S-VAPB, is linked to a dominantly inherited form of amyotrophic lateral sclerosis (ALS8). P56S-VAPB forms intracellular inclusions, whose role in ALS pathogenesis has not yet been elucidated. We recently demonstrated that these inclusions are formed by profoundly remodelled stacked ER cisternae. Here, we used stable HeLa-TetOff cell lines inducibly expressing wild-type VAPB and P56S-VAPB, as well as microinjection protocols in non-transfected cells, to investigate the dynamics of inclusion generation and degradation. Shortly after synthesis, the mutant protein forms small, polyubiquitinated clusters, which then congregate in the juxtanuclear region independently of the integrity of the microtubule cytoskeleton. The rate of degradation of the aggregated mutant is higher than that of the wild-type protein, so that the inclusions are cleared only a few hours after cessation of P56S-VAPB synthesis. At variance with other inclusion bodies linked to neurodegenerative diseases, clearance of P56S-VAPB inclusions involves the proteasome, with no apparent participation of macro-autophagy. Transfection of a dominant-negative form of the AAA ATPase p97/VCP stabilizes mutant VAPB, suggesting a role for this ATPase in extracting the aggregated protein from the inclusions. Our results demonstrate that the structures induced by P56S-VAPB stand apart from other inclusion bodies, both in the mechanism of their genesis and of their clearance from the cell, with possible implications for the pathogenic mechanism of the mutant protein.
Supply-Side Story: Risks, Guarantees, Competition, and Information Asymmetry
Authors: Gümüş Mehmet , Ray Saibal , Gurnani Haresh 
Published: May 2012

The risk of supply disruption increases as firms seek to procure from cheaper, but unproven, suppliers. We model a supply chain consisting of a single buyer and two suppliers, both of which compete for the buyer's order and face risk of supply disruption. One supplier is comparatively more reliable but also more expensive, whereas the other one is less reliable but cheaper and faces higher risk of disruption. Moreover, the risk level of the unreliable supplier may be private information, and this lack of visibility increases the buyer's purchasing risk. In such settings, the unreliable supplier often provides a price and quantity (P&Q) guarantee to the buyer. Our objective is to study the underlying motivation for the guarantee offer and its effects on the competitive intensity and the performance of the chain partners. Our model also includes a spot market that can be utilized by any party to buy or to sell. The spot market price is random, partially depends on the available capacity of the two suppliers, and has a positive spread between buying and selling prices. We analytically characterize the equilibrium contracts for the two suppliers and the buyer's optimal procurement strategy. First, our analysis shows that P&Q guarantee allows the unreliable supplier to better compete against the more reliable one by providing supply assurance to the buyer. More importantly, when information asymmetry risk is high, use of a guarantee may enable the unreliable supplier to credibly signal her true risk, thereby improving visibility into the chain. This signal can also be used by the buyer to infer the expected spot market price. In spite of these benefits, a guarantee offer in an asymmetric setting may not always be desirable for the buyer. Rather, it can reduce competition between the suppliers, resulting in higher costs for the buyer. This paper was accepted by Martin Lariviere, operations management.
Combining Equity and Utilitarianism in a Mathematical Programming Model
Authors: Hooker J. N. , Williams H. P. 
Published: May 2012

We discuss the problem of combining the conflicting objectives of equity and utilitarianism, for social policy making, in a single mathematical programming model. The definition of equity we use is the Rawlsian one of maximizing the minimum utility over individuals or classes of individuals. However, when the disparity of utility becomes too great, the objective becomes progressively utilitarian. Such a model is particularly applicable not only to health provision but to other areas as well. Building a mixed-integer/linear programming (MILP) formulation of the problem raises technical issues, because the objective function is nonconvex and the hypograph is not MILP representable in its initial form. We present a succinct formulation and show that it is “sharp” in the sense that its linear programming relaxation describes the convex hull of the feasible set (before extra resource allocation or policy constraints are added). We apply the formulation to a healthcare planning problem and show that instances of realistic size are easily solved by standard MILP software.This paper was accepted by Dimitris Bertsimas, optimization.