How Clot Composition Influences Fibrinolysis in the Acute Phase of Stroke: A Proteomic Study of Cerebral Thrombi
Authors: Doche Emilie , Sulowski Constance , Guigonis Jean-Marie , Graslin Fanny , Casolla Barbara , Hak Jean-François , Carle Xavier , Brunel Hervé , Lindenthal Sabine , Martin Jean-Charles , Pourcher Thierry , Suissa Laurent , Alessi Marie-Christine , Bobot Mickael , Brinet Abdelmalek , Dagnino Sonia , Dehondt Jean-Daniel , Legge Silvia Di , Dory-Lautrec Philippe , Dulau-Metras Cecile , Durozard Pierre , Kerleroux Basile , Laksiri Nadia , Leclercq Barbara , Lehmann Pierre , Magoncia Ellen , Manzac Christine , Osman Ophelie , Peres Roxane , Perez Sandra , Rey Caroline , Reyre Anthony , Robinet-Borgomano Emmanuelle , Snyman Salome , Svilar Ljubica , Tardivel Catherine , Tourniaire Patricia , Vingadalassalom Sivadji 
Published: May 2024

BACKGROUND:
The dramatic clinical improvement offered by mechanical thrombectomy raised questions about the relevance of prior intravenous thrombolysis in large-vessel occlusion strokes. Hence, studying intravenous thrombolysis susceptibility and its dependence on thrombus composition is crucial. We used an observational proteomic study of whole thrombi retrieved by mechanical thrombectomy to identify factors associated with fibrin content and fibrinolytic activity (FA).

METHODS:

In 104 stroke patients, the thrombi proteome was established by mass spectrometry coupled to liquid chromatography. FA was estimated in clots both outside (FA
out
) by measuring D-dimer levels at the blood-thrombus interface and inside (FA
in
) by evaluating the ratio of fibrinogen α to its plasmin-cleaved forms using proteomics coupled with protein electrophoresis. The factors associated with fibrin content, FA
in
, and FA
out
were determined by intravenous thrombolysis-adjusted linear regression.

RESULTS:

FA
out
(
P
<0.0001) and FA
in
(
P
=0.0147) were driven by recombinant tissue-type plasminogen activator (r-tPA) administration (47/104) and thrombus composition. Indeed, FA
out
was greater with fibrin-rich than erythrocyte-rich thrombi, presumably because of more (r)tPA substrates. Thus, FA
out
was increased with cardioembolic thrombi (72/104), which are rich in fibrin (
P
=0.0300). Opposite results were found inside the thrombus, suggesting that (r)tPA penetrability was hampered by the density of the fibrinous cap. Moreover, blood cells had a strong impact on thrombus structure and susceptibility to (r)tPA. Indeed, fibrin content was negatively associated with erythrocyte-specific proteins in the thrombus, admission hematocrit (
P
=0.0139), and hemoglobin level (
P
=0.0080), which underlines the key role of erythrocytes in thrombus composition. Also, an increased number of neutrophils impaired FA
out
(
P
=0.0225), which suggests that their aggregation around the thrombus prevented the (r)tPA attack. Only FA
out
was significantly associated with reduced thrombus weight (
P
=0.0310), increased recanalization rate (
P
=0.0150), good clinical outcome (
P
=0.0480), and reduced mortality (
P
=0.0080).

CONCLUSIONS:
Proteomics can offer new insights into the close relationship between thrombus composition and susceptibility to fibrinolysis, paving the way for new adjuvant therapies.