bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic heterogeneity of thalassemia major patients in Rembang Regency, Central Java, Indonesia
Authors: Widyastiti Nyoman Suci , Nainggolan Ita Margaretha , Limijadi Edward Kurnia Setiawan , Hendrianingtyas Meita , Retnoningrum Dwi , Ariosta  , Nency Yetty Movieta , Dewi Mayasari , Sutamti  , Ratnaningsih Tri , Sukorini Usi 
Published: May 2024

Link of Video Abstract: https://www.youtube.com/watch?v=RG95aJkUndg
Background: Thalassemia and hemoglobinopathies are caused by mutations in the globin genes that reduce haemoglobin synthesis. In many Asian countries, including Indonesia, thalassemia is a common inherited disorder. Thalassemia has specific characteristics due to the wide variability of the mutations from various populations. Therefore, screening protocol should be designed on specific populations’ regional gene mutation patterns. This study aims to investigate the mutational diversity of the globin genes in thalassemia major patients in Rembang Regency, Central Java, Indonesia.
Methods: Genetic mutations were carried out in all major thalassemia patients who were recorded and underwent routine blood transfusions at the Rembang District Hospital from 2018 – 2020. Mutation identification was carried out using a PCR- RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism) based method, the ACRS (Amplified Created Restriction Site) method, and genome sequencing.
Results: The most common genotypes are double heterozygous HbE and Cd 35 mutation (-C) (42.87%), HbE and IVS1-nt.5 mutations (G> C) (28.57%), HbE and IVS1- nt.2 mutations (T> C), HbE and codon 8/9 (insertion + G), codon 8/9 (insertion + G) and Hb Malay, as well as Hb Adana and Hb Constant Spring (1.74%).
Conclusion: The β-thalassemia and α-thalassemia mutations most commonly found in Rembang Regency are Cd 35 (-C) and non-deletional mutations.
Prostate specific antigen-age volume score in predicting prostate cancer among the Indonesian population
Authors: Hamid Agus Rizal Ardy Hariandy , Raharja Putu Angga Risky , Rahman Fakhri , Mochtar Chaidir Arif , Umbas Rainy 
Published: May 2024

Link of Video Abstract: https://youtu.be/ZNusQRjFEH0

Introduction: Several methods have been explored to increase the effectiveness of prostate-specific antigen (PSA) for prostate cancer screening. A lower prostate-specific antigen-age volume (PSA-AV) score is associated with higher cancer risk. This study aimed to evaluate the value of PSA-AV scores in the detection of prostate cancer among the Indonesian population.
Methods: A retrospective study was conducted on 844 prostate biopsies performed at our center between January 2015 and December 2019. The score of PSA-AV was derived by multiplication of the patient's age and prostate volume and then division with PSA level. A ROC curve analysis was conducted to determine the cutoff for the PSA-AV score. The sensitivities and specificities of the PSA-AV score were contrasted with total PSA, PSA density, and age-adjusted PSA.
Results: The mean age of the patient was 63.63±8.6 years, with a mean PSA of 16.3±13.9 ng/mL and a mean volume of 42.7±16.0 cm3. Among 844 biopsies, 25.5% tested positive for prostate cancer. Prostate cancer patients had significantly higher mean age, PSA levels, and positive digital rectal examinations (P<0.05). In predicting prostate cancer, the PSA-AV score demonstrated a higher AUC of 0.772, surpassing the AUC of 0.762 achieved by the total PSA. The analysis identified a cutoff of 700 for the PSA-AV score, which showed significant sensitivity (94%) and sufficient specificity (8.4%). These values outperformed the total PSA cutoff of 4 ng/mL (sensitivity 91.6%; specificity 5.4%), PSA density cutoff of 0.15 (sensitivity 85.1%; specificity 30.0%), and age-adjusted PSA cutoff (sensitivity 88.4%; specificity 11.8%). The superiority of the PSA-AV score of <700 was more prominent in the subgroup analysis of patients below 70 years of age and those with a low-moderate prostate volume (<60 cm3).
Conclusions: The PSA-AV score demonstrated superior sensitivities and specificities compared to total PSA, PSA density, and age-adjusted PSA. Therefore, it is recommended to utilize a PSA-AV score of <700, particularly for men below 70 years of age with low-moderate prostate volumes (< 60 cm3).
Transnasal Esophagoscopy (TNE) procedure in the outpatient clinic at Dr. Soetomo General Academic Hospital, Surabaya, Indonesia
Authors: Anantomo Muhammad Qodri , Perdana Rizka Fathoni 
Published: May 2024

Link of Video Abstract: https://youtu.be/nk1qtXzfl7I

Background: Transnasal esophagoscopy (TNE) is a small-caliber flexible esophagoscopy technique with the same diagnostic accuracy as conventional esophagoscopy. In addition, this technique is safer than any other technique and has patient tolerance. This study aims to analyze and evaluate the TNE procedure in patients.
Methods: a retrospective approach was used in this study by taking data from the activity register of the broncho-eschatology division of the outpatient unit of ORL-HNS Dr. Soetomo General Academic Hospital. TNE report book and patient medical records were collected to have the data. The observation period was from 2013 to 2017. Data were analyzed using SPSS version 25.0 for Windows.
Results: patients met the inclusion and exclusion criteria were 99 patients, with a male-to-female ratio of 2.3: 1. Most endoscopic findings were normal esophagus (56.57%). Existing abnormal findings included esophageal stenosis (18.18%), esophageal mucosal lesions (14.14%), and esophageal tumors (11.11%). TNE examination complications were found to be 1%.
Conclusion: TNE is widely used in patients aged 51-60, with dysphagia, reflux or Globus as the most common indications. TNE is quite safe to do in an outpatient hospital setting.
Clinical practice supportive psychotherapy for borderline personality disorder
Authors: Wardani Natalia Dewi , Widiastuti Maria Immaculata , Aris Sudiyanto  , Hardian  , Petrin Redayani Lukman  , Hertanto Wahyu Subagio  , Dodik Tugasworo  
Published: May 2024

Link of Video Abstract: https://youtu.be/rmJ-ivpSU3g

Borderline Personality Disorder is usually unstable due to periods of acute crisis, aggressive behavior, suicide attempts, and even substance abuse. Individual psychotherapy has long been considered the main treatment for Borderline Personality Disorder. Supportive psychotherapy can be used on people who are experiencing stressful situations that the person's coping mechanisms are unable to cope with, resulting in tension and distress. Individually supportive psychotherapy involves treating the patient with compassion, empathy, and commitment, regardless of whether the therapist agrees or disagrees with the patient's behavior and thoughts. Supportive psychotherapy seeks to overcome emotional reactivity to stressors and develop ways to inhibit maladaptive impulses. Supportive psychotherapy for Borderline Personality Disorder who have deficits in self-experience by focusing on the dimensions of impulsivity/aggression, affective instability, cognitive/perceptual distortions, and anxiety/inhibition. Supportive psychotherapy sessions discuss the four dimensions according to the patient's condition, which is expected to help patients overcome emotional reactivity and develop ways to inhibit maladaptive impulses.
Association between Human Papilloma Virus (HPV) genotype and mutant protein 53 (p53) expression in cervical cancer
Authors: Samuel  , Arifuddin Sharvianty , Lotisna David , Cangara M. Husni , Hamid Firdaus 
Published: May 2024

Link of Video Abstract:https://youtu.be/fff-Unnud2w

Background: Human Papilloma Virus (HPV) infection is the main cause of cervical cancer, a primary malignant tumor developing from squamous epithelial cells. HPV expresses oncoproteins E6 and E7, known to inactivate tumor suppressor proteins, one of which is protein 53 (p53). A promising biomarker for diagnosing and prognosis malignancies brought on by the HPV genotype is the identification of p53. This study investigates the association between p53 expression and HPV genotype in cervical cancer patients.
Method: This observational cross-sectional study involved patients diagnosed with cervical cancer on histopathological examination who met the requirements. The polymerase chain reaction (PCR) method was used to determine the HPV genotype, and an immunohistochemistry analysis was used to assess the level of p53 expression. A Chi-Square test is utilized to evaluate the association between the two variables.
Results: Of the 49 patients, there were 7 (14.3%) patients with HPV type 16, 13 (26.5%) with HPV type 18, 14 (26.5%) other types of HPV and 15 (30.6%) negatives for HPV. Examination of p53 expression showed that 17 (34.6%) samples had <10% expression, 17 (34.6%) had 10-50% expression, and 15 (30.8%) samples had >50% expression. There was no correlation between p53 expression and HPV genotype (p = 0.071). However, an association between p53 expression and cervical cancer's clinical stage was identified (p = 0.028).
Conclusion: Increased cervical cancer stage can be associated with increased p53 expression. Thus, p53 can be used as a predictor of cervical cancer stage. However, in cervical cancer, p53 expression cannot be associated with the HPV genotype.
Thoracic radiography in pediatric COVID-19 cases at Dr. Wahidin Sudirohusodo Hospital, Makassar in 2020-2021
Authors: Asriyani Sri , Sanre Eny , Zainuddin Andi Alfian , Santosa Jeremy Yoshua 
Published: May 2024

Link of Video Abstract: https://youtu.be/hCLGE3-eNjw

Background: Computed tomography (CT) as a chest radiographic examination has emerged as a crucial procedure for screening, diagnosing, and monitoring COVID-19 patients. This study aims to determine the radiographic appearance of thoracic imaging in pediatric cases of COVID-19 at Dr. Wahidin Sudirohusodo Hospital in Makassar, Indonesia.
Methods: This study is an observational study design that uses secondary data from medical records to examine the radiographic appearance of thoracic imaging in pediatric patients with COVID-19 infection. The study was conducted at the Radiology Department of Wahidin Sudirohusodo General Hospital in Makassar, Indonesia, from January 1st 2020, to December 31st 2021. The population included all pediatric patients with positive SARS-CoV-2 RT-PCR results and a chest X-ray examination sent for thoracic imaging examination. The study sample was taken from all accessible populations that met the study criteria until the end of the study period.
Results: A total of 147 samples (51.05%) showed abnormalities or pathological lesions on thoracic radiography, with consolidation being the most common finding (27.77%) distributed diffusely in both unilateral and bilateral regions. The rarest pathological finding was pleural effusion in 22 samples (7.63%). Conversely, 141 samples (48.95%) showed normal results on thoracic radiography examination. In the age group of 0-<1 month, 46 samples (55.43%) showed abnormalities on thoracic radiography, with consolidation being the most common finding in this age group, found in 30 samples (36.14%).
Conclusion: This study showed that most children with COVID-19 symptoms exhibit abnormalities on thoracic radiography. However, these findings are not specific, and therefore, thoracic radiography cannot be used for screening or as a first-line diagnostic examination.
Decrease in the number of Streptococcus mutans and Staphylococcus aureus bacterial colonies after administration of sentul fruit peel extract gel (Sandoricum koetjape) in gingivitis model of white Wistar rats
Authors: Wirata I Nyoman , Agung Anak Agung Gede , Arini Ni Wayan , Raiyanti I Gusti Ayu , Aryana I Ketut , Purna I Nyoman 
Published: May 2024

Link of Video Abstract: https://youtu.be/NpcREsYIlEE

Introduction: Sentul fruit peel extract (Sandoricum koetjape) has the ability to bacterium Streptococcus mutans and Staphylococcus aureus after administration of Sentul fruit peel extract gel on gingivitis model white Wistar rats. Utilization of extracts from the skin of sentul fruit (Sandoricum koetjape) as well as the proper use of natural sources will have an impact on the prevention and healing of gingivitis. The study aimed to determine the decrease in the number of Streptococcus mutans and Staphylococcus aureus bacteria colonies after administration of nano gel of sentul fruit peel extract in the gingivitis model of white Wistar rats.
Method: study design with experimental animal models. The data obtained by the One-Way ANOVA test and if the effect is significant continue with Duncan's test at the 5% level.
Result: There was a decrease in the number of Streptococcus mutans and Staphylococcus Aureus bacteria colonies after administration of Sentul fruit peel extract gel in the gingivitis model of white Wistar Rats. The best gel concentration was Sentul peel extract gel 2.4% with the smallest number of bacterial colonies, Staphylococcus aureus colonies of 2.2 x 102 ± 1.9 x 101 colony forming unit (cfu) and the number of Streptococcus mutans colonies of 2.6 x 102 ± 1.2 x 101 CFU.
Conclusion: Sentul fruit peel extract 2.4% effectively inhibits the growth of Streptococcus mutans and Staphylococcus Aureus in white Wistar rat models with gingivitis.
Assessing keloid treatment efficacy: a comparison of intralesional umbilical-cord mesenchymal stem cells, their conditioned medium, and triamcinolone acetonide injection through macroscopic and microscopic examination
Authors: Harsono Anastasia Dessy , Dilogo Ismail Hadisoebroto , Prasetyono Theddeus Octavianus Hari , Prasetyo Marcel , Werdhani Retno Asti , Jusman Sri Widia , Siregar Nuryati Chairani , Soedjana Hardisiswo 
Published: May 2024

Link Video Abstract: https://youtu.be/NAnsqP7dPd0

Background: Current keloid treatment involves intralesional injection of triamcinolone acetonide (TA), which, despite its usage, is associated with high recurrence rates and adverse effects. Mesenchymal stem cells (MSCs) exhibit potent proliferative abilities and can curb fibroblast activity and proliferation within keloids. It is now known that umbilical cord mesenchymal stem cells (UC-MSCs) have been shown to have greater proliferative potential than bone marrow-derived MSCs (BM-MSCs), and other advantages of UC-MSCs include being easily accessible and less immunogenic. To assess the viability of administering umbilical cord MSC (UC-MSC) and its conditioned medium (UC-CM) via intralesional injection compared to TA in reducing macroscopic keloid volume and type 1:3 collagen ratio.
Methods: This randomized controlled trial enrolled twenty-four keloid patients by consecutive sampling. Eligible patients were required to have keloids on the chest, back, abdomen, or extremities. Patients with hypertrophic scars, a history of kidney failure, hypertension, blood disorders, malignancy, pregnancy, breastfeeding, or keloid treatment were excluded. Sociodemographic data, keloid size, and tissue biopsies were collected during scheduled visits. Bivariate analyses applied were considered significant at a p-value of <0.05.
Results: The study revealed that the most significant decrease in macroscopic volume occurred within the UC-MSC group, followed by the UC-CM group, and then the TA group (UC-MSC: 50.24% ± 3.58%; UC-CM: 43.97% ± 3.04%; TA: 33.53% ± 2.64; p = 0.004. The UC-CM group exhibited the most substantial decrease in the type 1:3 collagen ratios (4.80±0.26), with UC-MSC (4.60(4.15-8.05)) and TA (3.96(1.63-4.14)) following in sequence (p=0.002).
Conclusion: The findings of this study indicate that the use of UC-MSC and UC-CM exhibits promising superiority over TA in terms of reducing macroscopic keloid volume and type 1:3 collagen ratio.
The role of matrix metalloproteinase-9 (MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) Expression in pelvic organ prolapse: A literature review
Authors: Anis Amilah , Kurniawati Eighty Mardiyan , Hardianto Gatut , Setyohadi Tri Hastono 
Published: May 2024

Link of Video Abstract: https://youtu.be/Zv9b2Zj96yk

Menopause is a physiological process as women get older. Urogenital syndrome, sexual difficulties, and pelvic organ prolapse (POP) are all common complaints among postmenopausal women, and these conditions can negatively impact their quality of life. There are still many unknowns regarding the pathophysiology and mechanisms of POP. Pelvic organ prolapse has been linked to the equilibrium of the extracellular matrix (ECM), which is controlled by matrix metalloproteinases and tissue inhibitors of metalloproteinases. Menopausal women experience a variety of symptoms due to hormonal changes, from urinary tract disturbance, vaginal atrophy, vaginal shortening, up to genital prolapse. Due to the collagen's diminished contractility, POP happens. Matrix metalloproteinase is responsible for breaking down collagen, but TIMP prevents MMP from doing its job. In women with POP caused by the breakdown of the collagen network, MMP-9 exhibits the greatest rise. In order to preserve the health of fibroblasts and collagen in postmenopausal women, increased expression of MMP-9 and decreased expression of TIMP-1 are necessary, which results in a decreased incidence of POP. The expression of MMP-9 in prolapse patients was significantly higher than control patients. In addition, TIMP-1 expression levels were significantly decreased in prolapse patients. Damage to the ECM's equilibrium is caused by increased MMP-9 expression and decreased expression of timp-1, which leads to clinical signs of pelvic organ prolapse.
Evaluation of degeneration retinal ganglion cell loss in acute glaucoma model rats using syringe pump technique
Authors: Siti Hajar  , Dessy Rakhmawati Emril  , Nurwasis  , Zinatul Hayati  , Dedy Syahrizal  
Published: May 2024

Link of Video Abstract: https://youtu.be/vaGQXY-i4KE

Introduction: Glaucoma is a neurodegenerative disorder marked by the gradual decline in function and degeneration of the retinal ganglion cells (RGCs) and their axons. The elevated intraocular pressure (IOP) is a predominant contributing factor to its onset. Employing laboratory animals to model glaucoma is a well-established strategy that serves the dual purpose of identifying disease mechanisms and developing novel neuroprotective approaches. The aim of this study was to develop a preclinical acute model of glaucoma based on controllable intraocular pressure with a syringe pump in rat Rattus norvegicus.
Methods: This research was an animal-experimental study in animal model adult male Wistar rat (Rattus norvegicus), taken in May - October 2023 in an integrated laboratory Faculty of Veterinary Medicine Universitas Syiah Kuala. Total samples used in this research were 10 rats, divided into two categories which are control group (A) and treatment group (B) were given a pressure of 250 mmHg pressure in the right eye for 60 minutes. Apoptosis and degeneration of retinal ganglion cells after 8 weeks of elevated intraocular a pressure of 250 mmHg in the right eye for 60 minutes were observed by the HE staining.
Result: A significant difference in IOP was noted between control group rat and treatment group rat. All rats that received treatment experienced an increase in IOP (100%). The RGC density decreased significantly in the treatment group rat (mean: 11.72 cell/field of view or lower 46.88%) compared with the control group (p =0.005).
Conclusion: A pressure of 250 mmHg for 60 minutes has been proven to cause degeneration of the RGCs up to nearly 50% within a week. We tried a technique to induce increased IOP in rats as a model of acute glaucoma. Syringe pump technique can effectively induce acute ocular hypertension in rats, which causes progressive loss of retinal ganglion cells.
On the Purpose and Structure of Basic Metho­dology for Investigating Crimes Related to the Activities of Foreign Citizens and Stateless Persons
Authors: Kuleshova Anna 
Published: May 2024

The article discusses key questions of the formation of a basic forensic methodology for investigating crimes involving foreign citizens and stateless persons — determining its goal, tasks and structural elements. The author analyzes various viewpoints regarding the system of special forensic techniques and presents her own opinion on this issue. It is determined that the basic level is the second one, it uses the general crime investigation method and presents a scientific-methodological foundation for the formation of special methodologies for investigating different crimes. The author formulates the criminalistic category “crimes connected with the activities of foreign citizens”, which makes it possible to present a definition of the basic methodology for investigating crimes connected with the activities of foreign citizens and stateless persons with the goal of creating effective opportunities for identifying, investigating, solving and preventing crimes connected with the activities of foreign citizens by developing general criminalistic means and methods of collecting, researching, evaluating and using forensically relevant information on a criminal case. The author determines the goals and key structural elements of the methodology that influence its contents.
On the Pragmatic Functions of the Idiomatic Expression ‘Tamam’ in Jordanian Arabic
Authors: Al- Hanaktah Isra'a , Hamdan Jihad M. 
Published: May 2024

This study investigates the pragmatic functions of the idiomatic expression Tamam (lit. ‘complete’) as used by university students who speak Jordanian Arabic (JA). To this end, the researchers developed an electronic questionnaire that contained 14 different situations/contexts, each illustrating one pragmatic function as identified and determined by the researchers. The questionnaire was distributed electronically via the WhatsApp application to 164 BA Jordanian students at The University of Jordan. The students were requested to pass intuitive judgements on the acceptability of these situations and related functions. The results showed that nine of the proposed functions were accepted by 70 per cent or more of the subjects, while five were accepted by 51-68 per cent. The former set of functions included expressing approval, seeking confirmation, showing understanding, expressing appreciation, showing joy and pleasure, showing tolerance, expressing sadness and submission, reassuring, and checking the attention of the addressee. The latter included expressing dissatisfaction, ending a conversation and expressing impatience, ridiculing/mocking, threatening, and expressing shock and disappointment. The study recommends that future research may examine the acceptability of such scenarios and functions among older generations (45+) with the aim of comparing the two aforementioned groups. Future research may also investigate the pragmatic functions of other idiomatic expressions in JA and other Arabic dialects.
Applying Genre-Based Approach to Enhance Vocational Students’ Achievement in Writing Procedure Text
Authors: Hardiningsih Sri , Tambunan Rika Wahyuni , Yusuf Muhammad , Rini Nur , Pandiya  
Published: May 2024

This research aimed to investigate the enhancement of students’ achievement in writing procedure text by implementing a genre-based approach. The research design of this research was action research. The location of the research is at Akademi Komunitas Negeri Putra Sang Fajar Blitar (AKNPSFB). The research instruments were writing sheet, interviews, observation checklists, and tests. This research analyzed 150 essays by applying descriptive analysis of statistics and essay content. One hundred and fifty students from English Course classes at AKNPSFB submitted their assignment which required them to write a procedure text for 60 minutes in 3 weeks for an English course with credit for 14 weeks. From the analysis, the students' average score continued to increase gradually from 65.60 in the pre-test to 72.15 in Cycle I and to 76.25 in Cycle II. This increase in scores proves that the application of the genre-based approach (GBA) is able to increase students' writing skills in procedure text.