bluuebunny/crossref_metadata_2025_split huggingface dataset:
XRCC1 gene polymorphisms and esophageal squamous cell carcinoma risk in Chinese population: A meta‐analysis of case–control studies
Authors: Dai Liping , Wang Kaijuan , Zhang Jianying , Lv Quanjun , Wu Xiaobing , Wang Yanping 
Published: March 2009

AbstractTwo non‐synonymous polymorphisms Arg194Trp and Arg399Gln in the DNA‐base excision repair gene X‐ray repair cross‐complementing group 1 (XRCC1) have been implicated in risk for esophageal cancer. However, the results from different studies remain controversial. The present meta‐analysis of literatures was performed to clarify these associations in Chinese population. A comprehensive literature search was conducted to identify all case–control studies ofXRCC1polymorphisms Arg194Trp and Arg399Gln and risk for esophageal squamous cell carcinoma (ESCC). A total of 9 eligible studies, including 1,538 ESCC cases and 2,472 controls, were identified to the meta‐analysis. The odds ratio (OR) for the variant homozygous genotype Trp/Trp of the Arg194Trp polymorphism, compared with the wild‐type homozygote Arg/Arg, was 1.59 (p= 0.0007), with 95% confidence interval (95% CI) 1.22–2.09, for ESCC risk without between‐study heterogeneity. However, there was no statistically significant associations of ESCC risk in the dominant model Arg/Trp+Trp/Trp (OR 0.97; 95% CI 0.84–1.12;p= 0.69) and heterozygous genotype Arg/Trp (OR 0.90; 95% CI 0.77–1.04;p= 0.16) when comparing with wild‐type genotype Arg/Arg. For Arg399Gln, there was no effect in dominant modeling (Arg/Gln+Gln/Glnvs.Arg/Arg: OR 0.92; 95% CI 0.80–1.06;p= 0.25), and the variant Gln/Gln homozygote was not associated with ESCC risk (OR 1.29; 95% CI 0.79–2.10;p= 0.31), either. In conclusion, Arg194Trp genetic polymorphism may be associated with an increased risk for developing ESCC and a study with the larger sample size is needed to further evaluate gene–environment interaction onXRCC1polymorphisms and ESCC risk. © 2009 UICC
A prospective analysis of the association between macronutrient intake and renal cell carcinoma in the European Prospective Investigation into Cancer and Nutrition
Authors: Allen Naomi E. , Roddam Andrew W. , Sieri Sabina , Boeing Heiner , Jakobsen Marianne Uhre , Overvad Kim , Tjønneland Anne , Halkjær Jytte , Vineis Paolo , Contiero Paolo , Palli Domenico , Tumino Rosario , Mattiello Amalia , Kaaks Rudolf , Rohrmann Sabine , Trichopoulou Antonia , Zilis Demosthenes , Koumantaki Yvoni , Peeters Petra H. , Bueno‐de‐Mesquita H. Bas , Barricarte Aurelio , Rodríguez Laudina , Dorronsoro Miren , Sánchez Maria‐José , Chirlaque María Dolores , Esquius Laura , Manjer Jonas , Wallström Peter , Ljungberg Börje , Hallmans Göran , Bingham Sheila , Khaw Kay‐Tee , Boffetta Paolo , Norat Teresa , Mouw Traci , Riboli Elio 
Published: March 2009

AbstractPrevious case‐control studies have suggested that a high intake of animal foods and its associated nutrients are associated with an increased risk of renal cell carcinoma, although data from prospective studies are limited. We report here on the relationship between macronutrient intake and renal cell carcinoma incidence among 435,293 participants enrolled in the European Prospective Investigation into Cancer and Nutrition. Cox proportional hazard models were used to examine the association of dietary intake of fat, protein, carbohydrate, fiber and cholesterol and risk of renal cell carcinoma adjusted for age, sex, center, height, body mass index, physical activity, education, smoking, menopausal status, alcohol and energy intake. During an average 8.8 years of follow‐up, 507 renal cell carcinoma cases occurred. Risk of renal cell carcinoma was not associated with macronutrient intake, including nutrients derived from animal sources. Our results indicate that macronutrient intake is not associated with risk of renal cell carcinoma in this cohort of European men and women. © 2009 UICC
IL‐8 induces exocytosis of arginase 1 by neutrophil polymorphonuclears in nonsmall cell lung cancer
Authors: Rotondo Rita , Barisione Gaia , Mastracci Luca , Grossi Francesco , Orengo Anna Maria , Costa Roberta , Truini Mauro , Fabbi Marina , Ferrini Silvano , Barbieri Ottavia 
Published: March 2009

AbstractArginase 1 (ARG1) inhibits T‐cell proliferation by degrading extracellular arginine, which results in decreased responsiveness of T cells to CD3/TCR stimulation. In humans, ARG1 is stored in inactive form within granules of polymorphonuclear neutrophils (PMNs) and gets activated on release. We studied the role of PMNs‐related ARG1 activity in nonsmall cell lung cancer (NSLC), in which tumor‐infiltrating lymphocytes showed reduced proliferation in response to CD3/TCR triggering. Patients with NSCLC had increased ARG1 plasma levels as compared to healthy controls. Furthermore, immunohistochemistry showed that tumor‐infiltrating PMNs display reduced intracellular ARG1, in comparison to intravascular or peritumoral PMNs, suggesting a role of tumor microenvironment in ARG1 release. Indeed, supernatants of NSCLC cell lines induced exocytosis of ARG1 from PMNs. All (4/4) NSCLC cell lines and all (7/7) CD14− cell samples from NSCLC expressed interleukin (IL)‐8 mRNA, whereas TNFα mRNA was expressed by 1 cell line and by 2 tumor specimens. Furthermore, all NSCLC cell lines secreted immunoreactive IL‐8, albeit at different levels. IL‐8 was as effective as TNFα in triggering ARG1 release and the 2 cytokines acted synergistically. Secreted ARG1 was biologically active and catabolized extracellular arginine. The supernatant of IL‐8 gene‐silenced NSCLC cells did not mediate ARG1 release by PMNs. Altogether these findings demonstrate a role of IL‐8 in ARG1 exocytosis by PMNs and indicate that, due at least in part to IL‐8 secreted by NSCLC cells, PMNs infiltrating NSCLC release ARG1. This phenomenon could contribute to local immune suppression. © 2009 UICC
Caveolin‐1 tumor‐promoting role in human melanoma
Authors: Felicetti Federica , Parolini Isabella , Bottero Lisabianca , Fecchi Katia , Errico Maria Cristina , Raggi Carla , Biffoni Mauro , Spadaro Francesca , Lisanti Michael P. , Sargiacomo Massimo , Carè Alessandra 
Published: March 2009

AbstractCaveolin‐1 (Cav‐1), a member of the caveolin family, regulates caveolae‐associated signaling proteins, which are involved in many biological processes, including cancer development. Cav‐1 was found to exert a complex and ambiguous role as oncogene or tumor suppressor depending on the cellular microenvironment. Here we investigated Cav‐1 expression and function in a panel of melanomas, finding its expression in all the cell lines. The exception was the primary vertical melanoma cell line, WM983A, characterized by the lack of Cav‐1, and then utilized as a recipient for Cav‐1 gene transduction to address a series of functional studies. The alleged yet controversial role of phospho (Ph)‐Cav‐1 on cell regulation was also tested by transducing the nonphosphorylatable Cav‐1Y14A mutant. Wild‐type Cav‐1, but not mutated Cav‐1Y14A, increased tumorigenicity as indicated by enhanced proliferation, migration, invasion and capacity of forming foci in semisolid medium. Accordingly, Cav‐1 silencing inhibited melanoma cell growth reducing some of the typical traits of malignancy. Finally, we detected a secreted fraction of Cav‐1 associated with cell released microvesicular particles able to stimulate in vitro anchorage independence, migration and invasion in a paracrine/autocrine fashion and, more important, competent to convey metastatic asset from the donor melanoma to the less aggressive recipient cell line. A direct correlation between Cav‐1 levels, the amount of microvesicles released in the culture medium and MMP‐9 expression was also observed. © 2009 UICC
Dietary intake of polyphenols, nitrate and nitrite and gastric cancer risk in Mexico City
Authors: Hernández‐Ramírez Raúl U. , Galván‐Portillo Marcia V. , Ward Mary H. , Agudo Antonio , González Carlos A. , Oñate‐Ocaña Luis F. , Herrera‐Goepfert Roberto , Palma‐Coca Oswaldo , López‐Carrillo Lizbeth 
Published: March 2009

AbstractN‐Nitroso compounds (NOC) are potent animal carcinogens and potential human carcinogens. The primary source of exposure for most individuals may be endogenous formation, a process that can be inhibited by dietary polyphenols. To estimate the risk of gastric cancer (GC) in relation to the individual and combined consumption of polyphenols and NOC precursors (nitrate and nitrite), a population‐based case–control study was carried out in Mexico City from 2004 to 2005 including 257 histologically confirmed GC cases and 478 controls. Intake of polyphenols, nitrate and nitrite were estimated using a food frequency questionnaire. High intakes of cinnamic acids, secoisolariciresinol and coumestrol were associated with an ∼50% reduction in GC risk. A high intake of total nitrite as well as nitrate and nitrite from animal sources doubled the GC risk. Odds ratios around 2‐fold were observed among individuals with both low intake of cinnamic acids, secoisolariciresinol or coumestrol and high intake of animal‐derived nitrate or nitrite, compared to high intake of the polyphenols and low animal nitrate or nitrite intake, respectively. Results were similar for both the intestinal and diffuse types of GC. Our results show, for the first time, a protective effect for GC because of higher intake of cinnamic acids, secoisolariciresinol and coumestrol, and suggest that these polyphenols reduce GC risk through inhibition of endogenous nitrosation. The main sources of these polyphenols were pears, mangos and beans for cinnamic acids; beans, carrots and squash for secoisolariciresinol and legumes for coumestrol. © 2009 UICC
Adipose tissue: A motor for the inflammation associated with obesity
Authors: Vachharajani Vidula , Granger D. Neil 
Published: March 2009

AbstractObesity is a worldwide epidemic that continues to grow at an alarming rate. This condition increases the morbidity and mortality associated with both acute and chronic diseases. Some of the deleterious consequences of obesity have been attributed to its induction of a low‐grade chronic inflammatory state that arises from the production and secretion of inflammatory mediators from the expanded pool of activated adipocytes. This review focuses on the mechanisms that underlie the proposed link between obesity and inflammation, and it addresses how obesity‐induced inflammation may account for increased morbidity and mortality that is associated with a diverse group of diseases. © 2009 IUBMB IUBMB Life, 61(4): 424–430, 2009
Simultaneous determination of maraviroc and raltegravir in human plasma by HPLC‐UV
Authors: Notari Stefania , Tommasi Chiara , Nicastri Emanuele , Bellagamba Rita , Tempestilli Massimo , Pucillo Leopoldo Paolo , Narciso Pasquale , Ascenzi Paolo 
Published: March 2009

AbstractTherapeutic drug monitoring is pivotal to improve the management of HIV infection. Here, a new HPLC–UV method to quantify simultaneously maraviroc and raltegravir levels in human plasma is reported. Remarkably, this is the first method for maraviroc determination in human plasma. The volume of the plasma sample was 600 μL. This method involved automated solid‐phase extraction with Oasis HLB Cartridge 1 cc (30 mg divinylbenzene and N‐vinylpyrrolidone) and evaporation in a water bath under nitrogen stream. The extracted samples were reconstituted with 200 μL 50/50 of mobile‐phase solution (0.01 M KH2PO4 and acetonitrile). Twenty microliters of these samples were injected into a HPLC–UV system, the analytes were eluted on an analytical dC18 Atlantis column (150 mm × 4.6 mm I.D.) with a particle size of 5 μm. The mobile phase (0.01 M KH2PO4 and acetonitrile) was delivered at 1.0 mL/min with isocratic elution. The total run time for a single analysis was 10 min; maraviroc and raltegravir were detected by UV at 197 and 300 nm. The calibration curves were linear up to 2,500 ng/mL. The absolute recovery ranged between 93 and 100%. The HPLC–UV method reported here has been validated and is currently applied to monitor plasma levels of maraviroc and raltegravir in HIV‐infected patients. © 2009 IUBMB IUBMB Life, 61(4):470–475, 2009
Metalation of metallothioneins
Authors: Ngu Thanh T. , Stillman Martin J. 
Published: March 2009

AbstractMetalloproteins represent ∼30% of all proteins known, yet our understanding of the structures of these metalloproteins, the metal content, and the mechanism for metalation are still very limited. One of the most studied metalloproteins is the ubiquitous metallothionein (MT), which in mammals contains two metal‐binding domains: a 9‐cysteine β domain and a 11‐cysteine α domain. Metals are coordinated in MT via the cysteinyl thiols present in the primary amino acid sequence and the geometry is controlled by the metal ion. This short review discusses the use of optical spectroscopy to study the metalation of MT with particular emphasis on the benefits and pitfalls involved. Further, the new properties of MT that have been revealed using electrospray ionization mass spectrometry in recent metalation studies will also be discussed. © 2009 IUBMB IUBMB Life, 61(4):438–446, 2009
Repair of segmental bone‐defect of goat's tibia using a dynamic perfusion culture tissue engineering bone
Authors: Wang Chaofeng , Wang Zhen , Li Aimin , Bai Feng , Lu Jianxi , Xu Shanglong , Li Dichen 
Published: March 2009

AbstractSegmental bone defect resulted from trauma or excision of bone tumor or pathology represents a common and significant clinical problem. In an attempt to solve this dilemma, we use β‐TCP combined with autologous bone marrow mesenchymal stem cells (auto‐BMSC) and cultured by dynamic perfusion to repair the segmental bone defects of goat's tibia. The β‐TCP scaffolds combined with auto‐BMSC by dynamic perfusion bioreactor or in static state were respectively transplanted into the defect (30 mm) of the goat tibias. The X‐ray films were gathered and analyzed at the different time points. At 24 weeks post‐operation, tissue engineering bones implanted were analyzed by histology and Micro‐CT. Results show that the capacity of osteogenesis in experimental group was higher than that of control group by X‐ray, histological, and micro‐CT analysis (p < 0.05). According to the study, we found that repair for segmental bone defects of tissue engineering bone cultured by dynamic perfusion culture bioreactor outweigh those cultured in static state. And we can conclude that this technology of tissue engineering bone will become a clinical method to the segmental bone‐defects repair in the future. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Implantation of a synthetic meniscal scaffold improves joint contact mechanics in a partial meniscectomy cadaver model
Authors: Brophy Robert H. , Cottrell Jocelyn , Rodeo Scott A. , Wright Timothy M. , Warren Russell F. , Maher Suzanne A. 
Published: March 2009

AbstractMeniscal substitutes should ideally distribute loads across adjacent articular cartilage much in the way of the native tissue, thereby protecting the cartilage from degeneration. The objective of this study was to quantify the loads transferred to the tibial plateau after implantation of a porous polyurethane scaffold. Our hypothesis was that scaffold implantation would improve the contact mechanics of a partial meniscectomy knee. Six sheep cadaver knees were mounted in a load‐controlled knee‐joint simulator. A pressure‐sensor sheet, secured to the tibial plateau, dynamically recorded pressure data over 10 gait cycles for the following conditions: (i) intact, (ii) partial meniscectomy, (iii) implanted scaffold, and (iv) total meniscectomy. Implantation of the scaffold increased the contact area and decreased the contact pressures compared to the meniscal defect knee. No significant difference was found between the average and peak contact pressures of the intact knees and scaffold‐implanted knees; however, contact area was significantly lower for the scaffold‐implanted knees compared to intact knees. Our hypothesis was accepted; replacement with a polyurethane scaffold improved knee‐contact mechanics relative to a partially meniscectomized knee. On the basis of these findings, the scaffold will be evaluated for its chondroprotective capacity in an in vivo ovine model. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
An understanding of enhanced osteoblast adhesion on various nanostructured polymeric and metallic materials prepared by ionic plasma deposition
Authors: Pareta Rajesh A. , Reising Alexander B. , Miller Tiffany , Storey Dan , Webster Thomas J. 
Published: March 2009

AbstractThe development of new materials through novel surface modification techniques to enhance orthopedic implant lifetimes (hence, decreasing the need for revision surgery) is of great interest to the medical community. The purpose of this in vitro study was to treat common metallic implant materials [such as titanium (Ti) and a titanium alloy (Ti6Al4V)] and traditional polymeric materials (like polyethylene terephthalate, polyvinyl chloride, polyurethane, polytetrafluoroethylene, ultra‐high molecular weight polyethylene (UHMWPE) and nylon) with either nanoparticulate alumina or titanium using novel (i) ionic plasma deposition (IPD) and (ii) nitrogen ion immersion plasma deposition (NIIPD) techniques. The treated surfaces were characterized by scanning electron microscopy, atomic force microscopy and surface energy, demonstrating greater nanoscale roughness on the modified surfaces regardless of the underlying material or coating applied. These surface‐modified substrates were also tested for cytocompatibility properties with osteoblasts (or bone‐forming cells). Results showed increased osteoblast adhesion on modified compared to control (traditional or untreated) materials. Since the adhesion of osteoblasts is the first crucial step for new bone synthesis, these results are very promising and suggest that the plasma deposition processes used in this study should be further investigated to improve the longevity of orthopedic implants. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Effect of a prostaglandin EP4 receptor agonist on early fixation of hydroxyapatite/titanium composite‐ and titanium‐coated rough‐surfaced implants in ovariectomized rats
Authors: Hayashi K. , Fotovati A. , Ali S. Abu , Nakamura Y. , Inagaki M. , Naito M. 
Published: March 2009

AbstractThe agonist of the prostaglandin EP4 receptor can increase bone density in osteoporosis. Using ovariectomized (OVX) and sham‐operated (SO) rats, the effects of the EP4 receptor agonist, ONO‐4819, and hydroxyapatite (HA) on implant‐bone fixation in implants with a rough surface were investigated. Female Wistar rats (12 weeks old) were divided into either SO or bilateral OVX groups. Twenty four weeks later, either hydroxyapatite/titanium (HA/Ti) composite‐coated or Ti‐coated implants were implanted into the femora, and the animals were treated with either ONO‐4819 or saline for 4 weeks. The fixation strength of the HA/Ti‐coated implants was higher than that of the Ti‐coated implants in the saline‐treated OVX rats. In the OVX rats, ONO‐4819 enhanced fixation of the rough Ti‐coated implants to levels similar to that of HA/Ti‐coated implants. These data suggest that a combination of treatment with an EP4 receptor agonist and a rough‐surfaced implant might be useful in increasing the early fixation of cement‐less arthroplasty, particularly in elderly patients with osteoporosis. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Distribution of ghrelin peptide in the gastrointestinal tract of stomachless and stomach‐containing teleosts
Authors: Arcamone Nadia , Neglia Simona , Gargiulo Giuliana , Esposito Vincenzo , Varricchio Ettore , Battaglini Pietro , De Girolamo Paolo , Russo Finizia 
Published: March 2009

AbstractThe occurrence and localization of ghrelin peptide in the gastrointestinal tract of Carassius auratus and Dicentrarchus labrax, two fish species that exhibit different feeding behavior, different habitats, and different anatomical organizations of the gastroenteric tract, were examined by immunohistochemical methods and western blotting analysis. All of the gastrointestinal segments studied displayed immunohistochemical localizations of ghrelin peptide. Numerous single or clustered immunoreactive cells were found along the gastric folds, particularly in the pyloric region of Dicentrarcus labrax, whereas scattered ghrelin immunoreactive cells were observed in the intestinal epithelium of both fish species. Double immunolabeling PGP 9.5/ghrelin demonstrated the localization of ghrelin peptide also in nerve fibers and neuronal cells of the submucosal and myenteric plexuses, often in association with vascular structures. Western blotting analysis confirmed the presence of ghrelin peptide in the gatrointestinal tract of both species studied, whose molecular weight was similar to that of the corresponding mammalian prepro‐ghrelin. The findings could support the hypothesis that this peptide is an important appetite regulator in fish and could confirm the presence of the ghrelin peptide, starting from its precursor proteins, in the gastrointestinal tract of the goldfish and the sea bass. Microsc. Res. Tech. 2009. © 2009 Wiley‐Liss, Inc.
Ultrastructural study of the ability of seminal plasma proteins to protect ram spermatozoa against cold‐shock
Authors: Colás Carmen , Junquera Concepción , Pérez‐Pé Rosaura , Cebrián‐Pérez José A. , Muiño‐Blanco Teresa 
Published: March 2009

AbstractThe process of sperm cryopreservation, involving cooling, freezing, and thawing, induces serious detrimental changes in sperm function. The plasma and acrosomal membranes of spermatozoa are considered to be the primary site of these modifications due to thermal, mechanical, chemical, and osmotic stress. In previous studies, we demonstrated the ability of seminal plasma (SP) proteins to protect ram spermatozoa against cold‐shock by using biochemical markers and scanning electron microscopy. In this study, we have attempted to examine the potential protective effect of SP proteins in membrane ultrastructure of ram spermatozoa subjected to cold‐shock, by means of transmission electron microscopy (TEM). All the experiments were carried out with fresh spermatozoa freed from SP by a dextran/swim‐up procedure. The high proportion of viable spermatozoa found in the swim‐up obtained sample decreased drastically after the cold‐shock treatment, and a considerable blebbing and vesiculation of the plasma and acrosomal membranes was found. The addition of SP proteins increased the sperm resistance to damage due to cold‐shock (48% membrane‐intact spermatozoa versus 15% in the control sample), and TEM analysis revealed that membrane alteration was prevented. This protective effect seems to be specific for SP proteins, as the addition of BSA did not provide any protection. Microsc. Res. Tech. 2009. © 2009 Wiley‐Liss, Inc.