bluuebunny/crossref_metadata_2025_split huggingface dataset:
Розвиток військово-історичного туризму в Україні
Authors: ГЛАДКИЙ Олександр , ГАШІМОВ Мікаель 
Published: June 2024

Розвиток військово-історичних компонен­тів в організації подорожей та дозвілля набув значного поширення в екскурсійній та туристично-рекреаційній справі, готель­ному та ресторанному бізнесі, що дає змогу широко використовувати його ресурси в діяльності туристичних підприємств. Військово-історичний туризм покликаний задовольнити всебічний інтерес туристів до військових знань і військової справи в наш час. Використовуючи військово-історичні туристичні знання, можна сформувати шанобливе ставлення до військовослужбовців та їх ролі у розвитку суспільства. Метою дослідження є розроблення ключових напрямів наукових стратегій формування та розвитку військово-історичного туризму як важливого елементу спеціалізованого туризму України, що виступає складовою отримання базової військової підготовки, фізичної підготовки для юнаків та дівчат до служби в ЗСУ, підготовки до самозахисту, отримання навичок володіння зброєю, а також елементом вшану­вання пам’яті загиблих захисників України. Це дослідження засноване на науково-діалектичному методі. Також основними методами дослідження є: метод описового аналізу, літературно-довідковий аналіз, аналі­тичний метод та науковий синтез. Розкрито особливості військово-історичного туризму як важливого напряму спеціалізованого туризму України. Наведено провідні результати класи­фікації військово-історич­ного туризму у працях українських учених. Визначено основні фактори, що впливають на розвиток військово-історичного туризму. Систематизовано ресурсну базу військово-історичного туризму України за регіонами та видовим складом. Проаналізовано ключові дестинації військово-історичного туризму різних регіонів України. Проведено деталь­ний аналіз стратегічних напрямів розвитку військово-історичного туризму в Україні з використанням методик SWOT-аналізу, аналізу макросередовища PESTLE та мікро­середовища COSMIC. Визначено основні стратегічні напрями розвитку військово-історичного туризму в Україні на період до 2030 р., систематизо­вано ключові завдання та напрями їх реалізації, окреслено очікувані результати формування та розвитку мережі військово-історичного туризму в Україні. Запропонована Стратегія напрямів розвитку військово-історичного туризму України передбачає розроблення широкої низки заходів соціального, політич­ного, ринкового, організаційного, краєзнавчого, природоохоронного характеру. Реалізація Стра­тегії спрямована на розвиток внутрішньої та зовнішньої політики України у сфері військово-історичного туризму, створення належних умов для зростання кількості туристів, що відвідують Україну, і, як результат, на покращення економічної ситуації в країні.
Peningkatan Nilai Tambah Mineral Kalsit Menjadi Barang Seni Lampu Hias
Authors: Ramadani Rully Nurhasan , Dinata M. Imam Aga , Adhitya Eka , Nuryahya Himawan , Widayati Sri 
Published: June 2024

Abstrak
Peningkatan nilai tambah mineral dan batuan dapat memberikan manfaat bagi masyarakat luas. Dengan kebijakan konservasi bahan galian dimana memiliki fokus optimalisasi manfaat dan minimalisasi dampak negatif usaha pertambangan. Didalam kerangka optimalisasi manfaat bahan galian terdapat upaya, pemeliharaan, proteksi, penambangan dan pengolahan serta peningkatan nilai tambah terhadap bahan galian tersebut. Mineral kalsit (CaCO3) termasuk kelompok mineral karbonat sebagai salah satu mineral penyusun utama batuan gamping. Pemanfaatan mineral kalsit ini ditinjau berdasarkan kualitas sifat fisik dan kandungan kimia yang terkandung. Pada prinsipnya penelitian ini memanfaatkan sifat fisik dari mineral tersebut dikarenakan kulitas kandungan kimia yang dikandung mineral kalsit tergolong rendah. Metode trial and error digunakan didalam penelitian ini dengan tujuan untuk mendapatkan desain barang seni yang tepat. Proses pengolahan yang sederhana serta tidak membutuhkan teknologi tinggi dan keahlian khusus yang bersertifikat, dengan memanfaatkan sifat transparani, warna dan kilap dari mineral kalsit akan menjadi barang seni yang memiliki nilai tambah ketika diberikan pencahayaan.

Kata kunci: Kalsit, Nilai tambah, Barang Seni, Lampu Hias
Musculoskeletal spine modeling in large patient cohorts: how morphological individualization affects lumbar load estimation
Authors: Lerchl Tanja , Nispel Kati , Bodden Jannis , Sekuboyina Anjany , El Husseini Malek , Fritzsche Christian , Senner Veit , Kirschke Jan S. 
Published: June 2024

Introduction: Achieving an adequate level of detail is a crucial part of any modeling process. Thus, oversimplification of complex systems can lead to overestimation, underestimation, and general bias of effects, while elaborate models run the risk of losing validity due to the uncontrolled interaction of multiple influencing factors and error propagation.Methods: We used a validated pipeline for the automated generation of multi-body models of the trunk to create 279 models based on CT data from 93 patients to investigate how different degrees of individualization affect the observed effects of different morphological characteristics on lumbar loads. Specifically, individual parameters related to spinal morphology (thoracic kyphosis (TK), lumbar lordosis (LL), and torso height (TH)), as well as torso weight (TW) and distribution, were fully or partly considered in the respective models according to their degree of individualization, and the effect strengths of these parameters on spinal loading were compared between semi- and highly individualized models. T-distributed stochastic neighbor embedding (T-SNE) analysis was performed for overarching pattern recognition and multiple regression analyses to evaluate changes in occurring effects and significance.Results: We were able to identify significant effects (p &lt; 0.05) of various morphological parameters on lumbar loads in models with different degrees of individualization. Torso weight and lumbar lordosis showed the strongest effects on compression (β ≈ 0.9) and anterior–posterior shear forces (β ≈ 0.7), respectively. We could further show that the effect strength of individual parameters tended to decrease if more individual characteristics were included in the models.Discussion: The induced variability due to model individualization could only partly be explained by simple morphological parameters. Our study shows that model simplification can lead to an emphasis on individual effects, which needs to be critically assessed with regard to in vivo complexity. At the same time, we demonstrated that individualized models representing a population-based cohort are still able to identify relevant influences on spinal loading while considering a variety of influencing factors and their interactions.
Effect of cement volume on biomechanical response of a spine segment treated with a PEEK polymer implant: a finite element comparative study with vertebroplasty
Authors: Vienney Cécile , Hambli Ridha , De Leacy Reade , Cornelis François H. 
Published: June 2024

In the current study, a 3D finite element study was performed to investigate the biomechanical response of an osteoporotic spine segment treated with a novel transpedicular implant (V-STRUT©, Hyprevention, France) made of PEEK (polyetheretherketone) material combined with either injections of 2, 3, 4, 5 and 6 cc of cement. The objective was to assess numerically the biomechanical performance of the implant in combination with different doses of the injected bone cement and to compare its performance with the gold standard vertebroplasty (VP) technique. A female (69 yo) was selected and a 3D finite element model of an osteoporotic spine segment was built based on a Computed Tomography (CT) scan performed from T12 to L2 with corresponding intervertebral discs and ligaments. A heterogeneous distribution of bone material properties was assigned to the bone using grey scale levels. Bilateral ellipsoid geometries of the inserted cement were retained for the V-STRUT and VP models based on experimental observation performed on different patients treated with the V-STRUT device. The current study demonstrated an optimal dose of 4 cc of bilaterally injected cement for the V-STRUT and VP techniques to restore the treated segment and confirmed that the V-STRUT device in combination with bone cement is superior to VP alone in establishing the normal stiffness and in reducing the applied stress to the immediately adjacent vertebral levels.
Genetically engineered electrospinning contributes to spinal cord injury repair by regulating the immune microenvironment
Authors: Sun Yang , Wu Jie , Zhou Liang , Wang Wei , Wang Haibo , Sun Shaosong , Xu Yichang , Zhang Lichen , Jiang Xinzhao , Zhu Guoqing , Xi Kun , Gu Yong , Chen Liang 
Published: June 2024

Introduction: Spinal cord injury (SCI) is associated with microenvironment imbalance, thereby resulting in poor regeneration and recovery of the spinal cord. Gene therapy can be used to balance the inflammatory response, however target genes cannot exist in localized injured areas.Methods: A genetically engineered electrospun scaffold (GEES) to achieve long-term immunoregulation and nerve repair was constructed. By combining the microfluidic and electrospinning techniques, interleukin-10 plasmid (pIL10) was loaded into lipid nanoparticles (LNPs) (pIL10-LNP), which was encapsulated to the nerve growth factor (NGF). Immunofluorescence staining, qRT-PCR, ELISA, flow cytometry, and other tests were employed to comprehensively assess the role of GEES in modulating macrophage polarization and facilitating neural repair.Results: The results showed that the scaffold released &gt;70% of the pIL10-LNP within 10 d and continued slow release within 30 d. In vitro cell experiments have demonstrated that GEES effectively stimulates macrophages to secrete anti-inflammatory cytokines and facilitates the differentiation of neural stem cells into neuronal cells. In rat T9 SCI model, the GEES significantly inhibited the inflammatory response in the acute and chronic phases of SCI by transfecting local tissues with slow-release pIL10-LNP to promote the release of the anti-inflammatory factor IL10, thereby creating a favorable microenvironment. With the addition of NGF, the repair and regeneration of nerve tissues was effectively promoted, and the post-SCI motor function of rats improved.Discussion: GEES can regulate post-SCI immune responses through continuous and effective gene delivery, providing a new strategy for the construction of electrospun scaffolds for nerve repair in gene therapy.
The multifaceted role of c-di-AMP signaling in the regulation of Porphyromonas gingivalis lipopolysaccharide structure and function
Authors: Ghods Shirin , Muszyński Artur , Yang Hyojik , Seelan Ratnam S. , Mohammadi Asal , Hilson Jacob S. , Keiser Griffin , Nichols Frank C. , Azadi Parastoo , Ernst Robert K. , Moradali Fata 
Published: June 2024

BackgroundThis study unveils the intricate functional association between cyclic di-3’,5’-adenylic acid (c-di-AMP) signaling, cellular bioenergetics, and the regulation of lipopolysaccharide (LPS) profile in Porphyromonas gingivalis, a Gram-negative obligate anaerobe considered as a keystone pathogen involved in the pathogenesis of chronic periodontitis. Previous research has identified variations in P. gingivalis LPS profile as a major virulence factor, yet the underlying mechanism of its modulation has remained elusive.MethodsWe employed a comprehensive methodological approach, combining two mutants exhibiting varying levels of c-di-AMP compared to the wild type, alongside an optimized analytical methodology that combines conventional mass spectrometry techniques with a novel approach known as FLATn.ResultsWe demonstrate that c-di-AMP acts as a metabolic nexus, connecting bioenergetic status to nuanced shifts in fatty acid and glycosyl profiles within P. gingivalis LPS. Notably, the predicted regulator gene cdaR, serving as a potent regulator of c-di-AMP synthesis, was found essential for producing N-acetylgalactosamine and an unidentified glycolipid class associated with the LPS profile.ConclusionThe multifaceted roles of c-di-AMP in bacterial physiology are underscored, emphasizing its significance in orchestrating adaptive responses to stimuli. Furthermore, our findings illuminate the significance of LPS variations and c-di-AMP signaling in determining the biological activities and immunostimulatory potential of P. gingivalis LPS, promoting a pathoadaptive strategy. The study expands the understanding of c-di-AMP pathways in Gram-negative species, laying a foundation for future investigations into the mechanisms governing variations in LPS structure at the molecular level and their implications for host-pathogen interactions.
Left ventricular structure and function following renal sympathetic denervation in patients with HFpEF: an echocardiographic 9-year long-term follow-up
Authors: Vogt Alexander , Plehn Alexander , Atti Carlo , Nussbaum Michael , Tongers Jörn , Sedding Daniel , Dutzmann Jochen 
Published: June 2024

BackgroundHigh blood pressure is a major risk factor for cardiac remodeling and left ventricular hypertrophy, increasing cardiovascular risk and leading to heart failure with preserved ejection fraction (HFpEF). Since renal sympathetic denervation (RDN) reduces blood pressure in the long term, we aimed to investigate the long-term effect of RDN in patients with HFpEF in the present analysis.MethodsPatients previously enrolled in a local RDN registry who underwent high-frequency RDN with the use of the Symplicity Flex® renal denervation system between 2011 and 2014 were followed up. The patients were assessed by 24-h ambulatory blood pressure measurement, transthoracic echocardiography, and laboratory tests. We used the echocardiographic and biomarker criteria of the Heart Failure Association (HFA)-PEFF (Pre-test assessment, Echocardiography and Natriuretic Peptide Score, Funkctional testing, and Final aetiology) score to identify patients with HFpEF.ResultsEchocardiographic assessment was available for 70 patients at a 9-year long-term follow-up. Of these patients, 21 had HFpEF according to the HFA-PEFF score. We found a significant reduction of the HFA-PEFF score from 5.48 ± 0.51 points at baseline to 4.33 ± 1.53 points at the 9-year follow-up (P &lt; 0.01). This decrease was due to a greater reduction in morphological and biomarker subcategories [from 1.95 ± 0.22 to 1.43 ± 0.51 points (P &lt; 0.01) and from 1.52 ± 0.52 to 0.90 ± 0.63 points (P &lt; 0.01), respectively] than in the functional one. Morphologically, there was a reduction in left ventricular hypertrophy and left atrial dilation.ConclusionsThe present analysis suggests that RDN may lead to a regression of the extent of HFpEF beyond a reduction in blood pressure and thus possibly contribute to an improvement in prognosis. More detailed information will be provided by ongoing randomized sham-controlled trials.
Prognostic models for mucinous and non-specific adeno cholangiocarcinoma: a population-based retrospective study
Authors: Azhar Muhammad Salman , Zhang Zi-jian , Liu Zhong-tao , Huang Yun-peng , Wang Yong-xiang , Zhou Hui , Xiong Li , Wen Yu , Zou Heng 
Published: June 2024

BackgroundClinically, the diagnosis and treatment of cholangiocarcinoma are generally different according to the location of occurrence, and the studies rarely consider the differences between different pathological types. Cholangiocarcinomas in large- and middle-sized intrahepatic bile ducts are mostly mucinous, while in small sized bile duct are not; mucinous extrahepatic cholangiocarcinomas are also more common than mucinous intrahepatic cholangiocarcinoma. However, it is unclear whether these pathological type differences are related to the prognosis.MethodsData of total 22509 patients was analyzed from Surveillance, Epidemiology, and End Results program database out of which 22299 patients were diagnosed with common adeno cholangiocarcinoma while 210 were diagnosed with mucinous cholangiocarcinoma. Based on the propensity score matching (PSM) analysis, between these two groups’ clinical, demographic, and therapeutic features were contrasted. The data were analyzed using Cox and LASSO regression analysis and Kaplan-Meier survival curves. Ultimately, overall survival (OS) and cancer specific survival (CSS) related prognostic models were established and validated in test and external datasets and nomograms were created to forecast these patients’ prognosis.ResultsThere was no difference in prognosis between mucinous cholangiocarcinoma and adeno cholangiocarcinoma. Therefore, we constructed prognostic model and nomogram that can be used for mucinous and adeno cholangiocarcinoma at the same time. By comparing the 9 independent key characteristics i.e. Age, tumor size, the number of primary tumors, AJCC stage, Grade, lymph node status, metastasis, surgery and chemotherapy, risk scores were calculated for each individual. By integrating these two pathological types in OS and CSS prognostic models, effective prognosis prediction results could be achieved in multiple datasets (OS: AUC 0.70–0.87; CSS: AUC 0.74–0.89).ConclusionAge, tumor size, the number of primary tumors, AJCC stage, Grade, lymph node status, metastasis, surgery and chemotherapy are the independent prognostic factors in OS or CSS of the patients with mucinous and ordinary cholangiocarcinoma. Nomogram that can be used for mucinous and adeno cholangiocarcinoma at the same time is of significance in clinical practice and management of cholangiocarcinoma.
Eight-day fasting modulates serum kynurenines in healthy men at rest and after exercise
Authors: Juhas Ulana , Reczkowicz Joanna , Kortas Jakub Antoni , Żychowska Małgorzata , Pilis Karol , Ziemann Ewa , Cytrych Inga , Antosiewicz Jędrzej , Borkowska Andżelika 
Published: June 2024

IntroductionTryptophan’s (Trp) metabolites are undervalued markers of human health. Their serum concentrations are modified by physical exercise and other factors, among which fasting has a well-documented role. Although this mechanism is hardly explored, thus, the study aimed to determine the effect of the 8-day fasting period and the impact of such a procedure on a single bout of an endurance exercise on the concentration of kynurenine pathway (KP) metabolites.Methods10 participants fasted for 8 days, and 10 as a control group participated in the study. The exercise was performed at baseline after an overnight fast and repeated post 8 days.ResultsThe 8 days of fasting increased the resting 3-hydroxy-L-kynurenine (3HK), picolinic acid (PA), kynurenic acid (KYNA), and xanthurenic acid (XA) serum concentration. Also elevated phenylalanine (Phe) and tyrosine (Tyr) levels were recorded, suggesting expanded proteolysis of muscle proteins. In turn, physical activity caused a decrease in the concentration of 3-hydroxyanthranilic acid (3HAA) and PA after fasting. The obtained results were not recorded in controls.ConclusionThe results of this study show that the health-promoting effects of fasting are associated with changes in the KYN pathway. The increase in the concentration of PA and XA metabolites following fasting is capable of penetrating the blood-brain barrier, and KYNA, which initiates several beneficial changes, supports this assumption.
PHE1-based IgG-like antibody platform provides a novel strategy for enhanced T-cell immunotherapy
Authors: Wang Lingbin , Jiang Haojie , Yin Xuying , Liang Tingting , Li Guoming , Ding Chen , Yang Mina , Zhang Lin , Liu Junling , Xu Yanyan 
Published: June 2024

IntroductionBispecific antibodies (BsAbs) can simultaneously target two epitopes of different antigenic targets, bringing possibilities for diversity in antibody drug design and are promising tools for the treatment of cancers and other diseases. T-cell engaging bsAb is an important application of the bispecific antibody, which could promote T cell-mediated tumor cell killing by targeting tumor-associated antigen (TAA) and CD3 at the same time.MethodsThis study comprised antibodies purification, Elisa assay for antigen binding, cytotoxicity assays, T cell activation by flow cytometry in vitro and xenogenic tumor model in vivo.ResultsWe present a novel bsAb platform named PHE-Ig technique to promote cognate heavy chain (HC)-light chain (LC) pairing by replacing the CH1/CL regions of different monoclonal antibodies (mAbs) with the natural A and B chains of PHE1 fragment of Integrin β2 based on the knob-in-hole (KIH) technology. We had also verified that PHE-Ig technology can be effectively used as a platform to synthesize different desired bsAbs for T-cell immunotherapy. Especially, BCMA×CD3 PHE-Ig bsAbs exhibited robust anti-multiple myeloma (MM) activity in vitro and in vivo.DiscussionMoreover, PHE1 domain was further shortened with D14G and R41S mutations, named PHE-S, and the PHE-S-based BCMA×CD3 bsAbs also showed anti BCMA+ tumor effect in vitro and in vivo, bringing more possibilities for the development and optimization of different bsAbs. To sum up, PHE1-based IgG-like antibody platform for bsAb construction provides a novel strategy for enhanced T-cell immunotherapy.
The efficacy of repetitive transcranial magnetic stimulation in postherpetic neuralgia: a meta-analysis of randomized controlled trials
Authors: Dai Qi , Xu Aihua , Wang Kunpeng , Yang Yang , Shao Yang , Sun Yongxin 
Published: June 2024

PurposeThis systematic review and meta-analysis aimed to evaluate the efficacy of repetitive transcranial magnetic stimulation (rTMS) in postherpetic neuralgia (PHN).MethodsThrough an extensive search in four databases until October 2023, we selected five randomized controlled trials adhering to our specific criteria, involving 257 patients in total. For continuous outcomes, the standardized mean difference (SMD) was calculated. Heterogeneity among the studies was assessed using Cochran’s I2 and Q statistics, adopting a random-effects model for I2 values over 50%. For assessing potential publication bias, we utilized both funnel plot and Egger’s test.ResultsOur analysis found that rTMS reduced the overall visual analogue scale (VAS) (SMD: −1.52, 95% CI: −2.81 to −0.23, p = 0.02), VAS at 1 month post-treatment (SMD: −2.21, 95% CI: −4.31 to −0.10, p = 0.04), VAS at 3 months post-treatment (SMD: −1.51, 95% CI: −2.81 to −0.22, p = 0.02), as well as patients’ global impression of change scale (PGIC) (SMD: −1.48, 95% CI: −2.87 to −0.09, p = 0.04) and short-form McGill pain questionnaire (SF-MPQ) (SMD: −1.25, 95% CI: −2.41 to −0.09, p = 0.03) compared to the sham-rTMS group.ConclusionOur study suggests that rTMS might have a potential alleviating effect on PHN symptoms. However, due to the limited number of studies and variations in rTMS parameters, larger sample studies involving more diverse populations, as well as further clarification of the most appropriate stimulation protocol, are still needed.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/, Identifier ID: CRD42023488420.