bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identifying the active content of interventions targeting the psychological well-being of carers of people with motor neuron disease: A systematic review
Authors: Cafarella Paul , Effing Tanja , Chur-Hansen Anna 
Published: June 2024

AbstractObjectivesThe primary aim of this research was to use a taxonomy of behavior change techniques (BCTTv1) to identify, map, and describe the active components of intervention and comparator groups in studies evaluating the psychological well-being (PWB) of motor neuron disease (MND) carers. Secondary aims were to (a) identify absent active ingredients and (b) explore whether variability in the effectiveness of interventions targeting the PWB of MND carers could be better explained through improved characterization of the active content of these interventions.MethodsMixed-methods systematic review based on Joanna Briggs Institute methodology for quantitative, qualitative, and mixed-methods reviews and Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Content-coding of interventions targeting the PWB of MND carers using BCTTv1 was conducted.ResultsSixteen manuscripts describing 14 studies were included. Forty-one of the possible 93 behavior change techniques (BCTs, 44%) were identified as active ingredients, while 52 BCTs (56%) were absent. BCTs were identified in all 14 intervention groups and 4 control groups. Four of the 16 overall BCTTv1 categories were absent. Eleven of the 14 studies demonstrated PWB benefits from their interventions.Significance of resultsIdentified and absent BCTs and BCTTv1 categories were mapped for all study groups, enabling a transparent characterization of active intervention content associated with positive PWB outcomes. Directions to improve interventions in this nascent field of research included the investigation of relevant untested BCTs in this population and the management of reporting and methodological quality issues.
Biologically effective dose of diflufenican applied preemergence for the control of multiple herbicide–resistant waterhemp in corn
Authors: Soltani Nader , Willemse Christian , Sikkema Peter H. 
Published: June 2024

Abstract
Waterhemp is a dioecious species with wide genetic diversity which has enabled it to evolve resistance to several commonly used herbicide groups in North America. Five field trials were established in Ontario to ascertain the biologically effective doses of diflufenican, a new Group 12 herbicide applied preemergence for control of multiple herbicide–resistant (MHR) waterhemp in corn. Based on regression analysis, the predicted diflufenican doses to elicit 50%, 80%, and 95% MHR waterhemp control were 99, 225, and 417 g ai ha−1, respectively, at 2 wk after application (WAA); 73, 169, and 314 g ai ha−1, respectively, at 4 WAA; and 76, 215, and — (meaning the effective dose was beyond the set of doses in this study) g ai ha−1, respectively, at 8 WAA. The predicted diflufenican doses that would cause a 50%, 80%, and 95% decreases in MHR waterhemp density were 42, 123, and — g ai ha−1; and MHR waterhemp biomass were 72, 167, and 310 g ai ha−1, respectively, at 8 WAA. Diflufenican applied preemergence at 150 g ai ha−1 controlled MHR waterhemp by 64%, 79%, and 73% at 2, 4, and 8 WAA, respectively. Isoxaflutole + atrazine applied preemergence at 105 + 1,060 g ai ha−1 controlled MHR waterhemp by 98%, 98%, and 97% at 2, 4, and 8 WAA, respectively; and S-metolachlor/mesotrione/bicyclopyrone/atrazine applied preemergence at 1,259/140/35/588 g ai ha−1 controlled MHR waterhemp by 100%, 100%, and 99% at 2, 4, and 8 WAA, respectively. Diflufenican applied preemergence reduced MHR waterhemp density and biomass by 83%; in contrast, isoxaflutole + atrazine and S-metolachlor/mesotrione/bicyclopyrone/atrazine reduced MHR waterhemp density and biomass by 99%. All treatments evaluated caused either no, or minimal, corn injury and resulted in corn yield that was similar with the weed-free control. Results indicate that diflufenican applied alone preemergence does not provide superior MHR waterhemp control over the commonly used herbicides isoxaflutole + atrazine or S-metolachlor/mesotrione/bicyclopyrone/atrazine; however, there is potential for using diflufenican as part of an integrated weed management strategy for the control of MHR waterhemp control in corn.
Projecting Changes in the Frequency and Magnitude of Ozone Pollution Events Under Uncertain Climate Sensitivity
Authors: East James D. , Monier Erwan , Saari Rebecca K. , Garcia‐Menendez Fernando 
Published: June 2024

AbstractClimate change is projected to worsen ozone pollution over many populated regions, with larger impacts at higher concentrations. More intense and frequent ozone episodes risk setbacks to human health and environmental policy achievements. However, assessing these changes is complicated by uncertain climate sensitivity, closely related to climate model response, and internal variability in simulations projecting climate's influence on air quality. Here, leveraging a global modeling framework that one‐way couples a human activity model, an Earth system model of intermediate complexity, and an atmospheric chemistry model, we investigate the role of climate sensitivity in climate‐induced changes to high ozone pollution episodes in the United States using multiple greenhouse gas emissions scenarios, representations of climate sensitivity, and initial condition members. We bias correct and evaluate historical model simulations, identifying modeled and observed O3 episodes using extreme value theory, and extend the approach to projections of mid‐ and end‐century climate impacts. Results show that the influence of climate sensitivity can be as significant as that of greenhouse gas emissions scenario absent precursor emissions changes. Climate change is projected to increase the magnitude of the highest annually occurring O3 concentrations by over 2.3 ppb on average across the U.S. at mid‐century under a high climate sensitivity and moderate emissions scenario, but the increase is limited to less than 0.3 ppb under lower climate sensitivity. Further, we show that areas in the U.S. currently meeting air quality standards risk being pushed into non‐compliance due to a climate‐induced increase in frequency of high ozone days.
Decoupling Warming and Monsoon Precipitation Over the Last 3110 Years in Northwestern Yunnan Province, Southwestern China
Authors: Jia Baoyan , Xiao Xiayun , Chi Changting , Ke Rui , Li Yanling 
Published: June 2024

AbstractUnderstanding the variability and related dynamic mechanisms of the Indian summer monsoon evolution over the past millenniums is crucial for disaster prevention and mitigation in the context of strengthening global warming. Here, we present a high‐resolution hydroclimate reconstruction over the past 3110 years in northwestern Yunnan Province, southwestern China, based on grain size analysis using the End‐member modeling algorithm and other environmental proxies. The results indicate that the temperature in northwestern Yunnan Province generally decreased since 3110 cal. yr BP, aligning with other temperature records on regional and global scales. Precipitation in the study area remained relatively stable before 1000 cal. yr BP, but exhibited an obvious out‐phase change compared with the temperature records over multidecadal to centennial timescale. While after 1000 cal. yr BP, the precipitation steadily increased, revealing the hydroclimate pattern of notably dry Medieval Warm Period (MWP) and relatively wet Little Ice Age (LIA) in the study area. The decoupling combination of temperature and precipitation spanning the MWP and LIA in southwestern China contrasts with that in the East Asian summer monsoon area and the Indian subcontinent which is also controlled by the Indian summer monsoon, indicating the spatial heterogeneity of precipitation in the Asian summer monsoon region. The discussion about dynamic mechanisms points out that solar activity emerges as a significant factor influencing regional temperature changes, and El Niño‐Southern Oscillation and Intertropical Convergence Zone migration jointly impact the precipitation change in the study area and the hydroclimate discrepancy in the Asian summer monsoon region.
Sedimentary Organic Nitrogen Isotopic Constraints on the Mid‐Holocene Transition in the Nitrogen Dynamics of the Northern South China Sea
Authors: Ye Feng , Wang Liwei , Wei Gangjian , Shi Xuesong , Huang Chao 
Published: June 2024

AbstractMillennial‐scale nitrogen (N) cycling processes in marginal seas and their response to climate change have not been well understood. Here, we present high‐resolution (ca. 110 years) organic nitrogen isotope (δ15Norg) data since the last deglaciation (16.1 ka) derived from a highly resolved sediment core in the northern South China Sea, aiming to explore millennial‐scale N cycling processes in this area. Unlike most bulk nitrogen isotope (δ15Nbulk) records from the South China Sea, the δ15Norg records show a clear response to well‐defined climatic episodes during the last deglaciation and early Holocene (EH, ∼11.7 to 9 ka), but exhibit a gradually decreasing trend in mid‐to‐late Holocene (since ca. 9 ka). During the last deglaciation and EH, the upper water column N dynamics are controlled by the lateral transport of surface nitrate from eastern tropical Pacific (ETP) presumably via the North Pacific Intermediate Water (NPIW) and to some extent, influenced by the altered input of terrigenous matter driven by sea level change. The significant decrease in δ15Norg since the mid‐Holocene (at ca. 9 ka) can be best explained by the increase in local N2 fixation forced by enhanced El Niño. This mechanism is consistent with modern observations. Overall, our results may reflect the main controlling factors of surface ocean N dynamics have shifted from zonal transport of nitrate from the ETP to El Niño since the mid‐Holocene.
Revisiting Seismic Energy of Shallow Tremors: Amplifications Due To Site and Propagation Path Effects Near the Nankai Trough
Authors: Takemura Shunsuke , Emoto Kentaro , Yabe Suguru 
Published: June 2024

AbstractWe investigated the effects of the propagation path and site amplification of shallow tremors along the Nankai Trough. Using far‐field S‐wave propagation from intraslab earthquake data, the amplification factors at the DONET stations were 5–40 times against an inland outcrop rock site. Thick (∼5 km) sedimentary layers with VS of 0.6–2 km/s beneath DONET stations have been confirmed by seismological studies. To investigate the effects of thick sedimentary layers, we synthesized seismograms of shallow tremors and intraslab earthquakes at seafloor stations. The ratios of the maximum amplitudes from the synthetic intraslab seismograms between models with and without thick sedimentary layers were 1–2. This means that thin lower‐velocity (<0.6 km/s) sediments just below the stations primarily control the estimated large amplifications. Conversely, at near‐source (≤20 km) distances, 1‐order amplifications of seismic energies for a shallow tremor source can occur due to thick sedimentary layers. Multiple S‐wave reflections between the seafloor and plate interface are contaminated in tremor envelopes; consequently, seismic energy and duration are overestimated. If a shallow tremor occurs within underthrust sediments, the overestimation becomes stronger because of the invalid rigidity assumptions around the source region. After 1‐order corrections of seismic energies of shallow tremors along the Nankai Trough, the scaled energies of seismic slow earthquakes were 10−10–10−9 irrespective of the region and source depth. Hence, the physical mechanisms governing seismic slow earthquakes can be the same, irrespective of the region and source depth.
RGS19 activates the MYH9/β-catenin/c-Myc positive feedback loop in hepatocellular carcinoma
Authors: Ke Shanjia , Lu Shounan , Xu Yanan , Bai Miaoyu , Yu Hongjun , Yin Bing , Wang Chaoqun , Feng Zhigang , Li Zihao , Huang Jingjing , Li Xinglong , Qian Baolin , Hua Yongliang , Fu Yao , Sun Bei , Wu Yaohua , Ma Yong 
Published: June 2024

AbstractHepatocellular carcinoma (HCC) is one of the most common fatal cancers worldwide, and the identification of novel treatment targets and prognostic biomarkers is urgently needed because of its unsatisfactory prognosis. Regulator of G-protein signaling 19 (RGS19) is a multifunctional protein that regulates the progression of various cancers. However, the specific function of RGS19 in HCC remains unclear. The expression of RGS19 was determined in clinical HCC samples. Functional and molecular biology experiments involving RGS19 were performed to explore the potential mechanisms of RGS19 in HCC. The results showed that the expression of RGS19 is upregulated in HCC tissues and is significantly associated with poor prognosis in HCC patients. RGS19 promotes the proliferation and metastasis of HCC cells in vitro and in vivo. Mechanistically, RGS19, via its RGS domain, stabilizes the MYH9 protein by directly inhibiting the interaction of MYH9 with STUB1, which has been identified as an E3 ligase of MYH9. Moreover, RGS19 activates β-catenin/c-Myc signaling via MYH9, and RGS19 is also a transcriptional target gene of c-Myc. A positive feedback loop formed by RGS19, MYH9, and the β-catenin/c-Myc axis was found in HCC. In conclusion, our research revealed that competition between RGS19 and STUB1 is a critical mechanism of MYH9 regulation and that the RGS19/MYH9/β-catenin/c-Myc feedback loop may represent a promising strategy for HCC therapy.
METTL3-mediated pre-miR-665/DLX3 m6A methylation facilitates the committed differentiation of stem cells from apical papilla
Authors: Gu Tingjie , Guo Rong , Fang Yuxin , Xiao Ya , Chen Luyao , Li Na , Ge Xingyun Kelesy , Shi Yijia , Wu Jintao , Yan Ming , Yu Jinhua , Li Zehan 
Published: June 2024

AbstractMethyltransferase-like 3 (METTL3) is a crucial element of N6-methyladenosine (m6A) modifications and has been extensively studied for its involvement in diverse biological and pathological processes. In this study, we explored how METTL3 affects the differentiation of stem cells from the apical papilla (SCAPs) into odonto/osteoblastic lineages through gain- and loss-of-function experiments. The m6A modification levels were assessed using m6A dot blot and activity quantification experiments. In addition, we employed Me-RIP microarray experiments to identify specific targets modified by METTL3. Furthermore, we elucidated the molecular mechanism underlying METTL3 function through dual-luciferase reporter gene experiments and rescue experiments. Our findings indicated that METTL3+/− mice exhibited significant root dysplasia and increased bone loss. The m6A level and odonto/osteoblastic differentiation capacity were affected by the overexpression or inhibition of METTL3. This effect was attributed to the acceleration of pre-miR-665 degradation by METTL3-mediated m6A methylation in cooperation with the “reader” protein YTHDF2. Additionally, the targeting of distal-less homeobox 3 (DLX3) by miR-665 and the potential direct regulation of DLX3 expression by METTL3, mediated by the “reader” protein YTHDF1, were demonstrated. Overall, the METTL3/pre-miR-665/DLX3 pathway might provide a new target for SCAP-based tooth root/maxillofacial bone tissue regeneration.
Advances and challenges in modeling inherited peripheral neuropathies using iPSCs
Authors: Van Lent Jonas , Prior Robert , Pérez Siles Gonzalo , Cutrupi Anthony N. , Kennerson Marina L. , Vangansewinkel Tim , Wolfs Esther , Mukherjee-Clavin Bipasha , Nevin Zachary , Judge Luke , Conklin Bruce , Tyynismaa Henna , Clark Alex J. , Bennett David L. , Van Den Bosch Ludo , Saporta Mario , Timmerman Vincent 
Published: June 2024

AbstractInherited peripheral neuropathies (IPNs) are a group of diseases associated with mutations in various genes with fundamental roles in the development and function of peripheral nerves. Over the past 10 years, significant advances in identifying molecular disease mechanisms underlying axonal and myelin degeneration, acquired from cellular biology studies and transgenic fly and rodent models, have facilitated the development of promising treatment strategies. However, no clinical treatment has emerged to date. This lack of treatment highlights the urgent need for more biologically and clinically relevant models recapitulating IPNs. For both neurodevelopmental and neurodegenerative diseases, patient-specific induced pluripotent stem cells (iPSCs) are a particularly powerful platform for disease modeling and preclinical studies. In this review, we provide an update on different in vitro human cellular IPN models, including traditional two-dimensional monoculture iPSC derivatives, and recent advances in more complex human iPSC-based systems using microfluidic chips, organoids, and assembloids.
Dissecting the MUC5AC/ANXA2 signaling axis: implications for brain metastasis in lung adenocarcinoma
Authors: Chaudhary Sanjib , Siddiqui Jawed Akhtar , Appadurai Muthamil Iniyan , Maurya Shailendra Kumar , Murakonda Swathi P. , Blowers Elizabeth , Swanson Ben J. , Nasser Mohd Wasim , Batra Surinder K. , Lakshmanan Imayavaramban , Ganti Apar Kishor 
Published: June 2024

AbstractNon-small cell lung carcinoma (NSCLC) exhibits a heightened propensity for brain metastasis, posing a significant clinical challenge. Mucin 5ac (MUC5AC) plays a pivotal role in the development of lung adenocarcinoma (LUAD); however, its role in causing brain metastases remains unknown. In this study, we aimed to investigate the contribution of MUC5AC to brain metastasis in patients with LUAD utilizing various brain metastasis models. Our findings revealed a substantial increase in the MUC5AC level in LUAD brain metastases (LUAD-BrM) samples and brain-tropic cell lines compared to primary samples or parental control cell lines. Intriguingly, depletion of MUC5AC in brain-tropic cells led to significant reductions in intracranial metastasis and tumor growth, and improved survival following intracardiac injection, in contrast to the observations in the control groups. Proteomic analysis revealed that mechanistically, MUC5AC depletion resulted in decreased expression of metastasis-associated molecules. There were increases in epithelial-to-mesenchymal transition, tumor invasiveness, and metastasis phenotypes in tumors with high MUC5AC expression. Furthermore, immunoprecipitation and proteomic analysis revealed a novel interaction of MUC5AC with Annexin A2 (ANXA2), which activated downstream matrix metalloproteases and facilitated extracellular matrix degradation to promote metastasis. Disrupting MUC5AC-ANXA2 signaling with a peptide inhibitor effectively abrogated the metastatic process. Additionally, treatment of tumor cells with an astrocyte-conditioned medium or the chemokine CCL2 resulted in upregulation of MUC5AC expression and enhanced brain colonization. In summary, our study demonstrates that the MUC5AC/ANXA2 signaling axis promotes brain metastasis, suggesting a potential therapeutic paradigm for LUAD patients with high MUC5AC expression.
Factor analysis of lifetime psychopathology and its brain morphometric and genetic correlates in a transdiagnostic sample
Authors: Krug Axel , Stein Frederike , David Friederike S. , Schmitt Simon , Brosch Katharina , Pfarr Julia-Katharina , Ringwald Kai G. , Meller Tina , Thomas-Odenthal Florian , Meinert Susanne , Thiel Katharina , Winter Alexandra , Waltemate Lena , Lemke Hannah , Grotegerd Dominik , Opel Nils , Repple Jonathan , Hahn Tim , Streit Fabian , Witt Stephanie H. , Rietschel Marcella , Andlauer Till F. M. , Nöthen Markus M. , Philipsen Alexandra , Nenadić Igor , Dannlowski Udo , Kircher Tilo , Forstner Andreas J. 
Published: June 2024

AbstractThere is a lack of knowledge regarding the relationship between proneness to dimensional psychopathological syndromes and the underlying pathogenesis across major psychiatric disorders, i.e., Major Depressive Disorder (MDD), Bipolar Disorder (BD), Schizoaffective Disorder (SZA), and Schizophrenia (SZ). Lifetime psychopathology was assessed using the OPerational CRITeria (OPCRIT) system in 1,038 patients meeting DSM-IV-TR criteria for MDD, BD, SZ, or SZA. The cohort was split into two samples for exploratory and confirmatory factor analyses. All patients were scanned with 3-T MRI, and data was analyzed with the CAT-12 toolbox in SPM12. Psychopathological factor scores were correlated with gray matter volume (GMV) and cortical thickness (CT). Finally, factor scores were used for exploratory genetic analyses including genome-wide association studies (GWAS) and polygenic risk score (PRS) association analyses. Three factors (paranoid-hallucinatory syndrome, PHS; mania, MA; depression, DEP) were identified and cross-validated. PHS was negatively correlated with four GMV clusters comprising parts of the hippocampus, amygdala, angular, middle occipital, and middle frontal gyri. PHS was also negatively associated with the bilateral superior temporal, left parietal operculum, and right angular gyrus CT. No significant brain correlates were observed for the two other psychopathological factors. We identified genome-wide significant associations for MA and DEP. PRS for MDD and SZ showed a positive effect on PHS, while PRS for BD showed a positive effect on all three factors. This study investigated the relationship of lifetime psychopathological factors and brain morphometric and genetic markers. Results highlight the need for dimensional approaches, overcoming the limitations of the current psychiatric nosology.
A multi-site 99mTc-HMPAO SPECT study of cerebral blood flow in a community sample of patients with major depression
Authors: Peterson Bradley S. , Li Jennifer , Trujillo Manuel , Sawardekar Siddhant , Balyozian David , Bansal Siddharth , Sun Bernice F. , Marcelino Courtney , Nanda Anoop , Xu Tracy , Amen Daniel , Bansal Ravi 
Published: June 2024

AbstractPrior regional Cerebral Blood Flow (rCBF) studies in Major Depressive Disorder (MDD) have been limited by small, highly selective, non-representative samples that have yielded variable and poorly replicated findings. The aim of this study was to compare rCBF measures in a large, more representative community sample of adults with MDD and healthy control participants. This is a cross-sectional, retrospective multi-site cohort study in which clinical data from 338 patients 18–65 years of age with a primary diagnosis of MDD were retrieved from a central database for 8 privately owned, private-pay outpatient psychiatric centers across the United States. Two 99mTc-HMPAO SPECT brain scans, one at rest and one during performance of a continuous performance task, were acquired as a routine component of their initial clinical evaluation. In total, 103 healthy controls, 18–65 years old and recruited from the community were also assessed and scanned. Depressed patients had significantly higher rCBF in frontal, anterior cingulate, and association cortices, and in basal ganglia, thalamus, and cerebellum, after accounting for significantly higher overall CBF. Depression severity associated positively with rCBF in the basal ganglia, hippocampus, cerebellum, and posterior white matter. Elevated rCBF was especially prominent in women and older patients. Elevated rCBF likely represents pathogenic hypermetabolism in MDD, with its magnitude in direct proportion to depression severity. It is brain-wide, with disproportionate increases in cortical and subcortical attentional networks. Hypermetabolism may be a reasonable target for novel therapeutics in MDD.