bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effect of rhPTH on the healing of tendon to bone in a rat model
Authors: Hettrich Carolyn M. , Beamer Brandon S. , Bedi Asheesh , Deland Kate , Deng Xiang‐Hua , Ying Liang , Lane Joseph , Rodeo Scott A. 
Published: November 2011

AbstractSuccessful rotator cuff tendon repair depends on secure tendon‐to‐bone healing. Recombinant human parathyroid hormone (rhPTH) has been shown in multiple studies to accelerate bone healing. Recent studies have also shown that rhPTH is chondrogenic by increasing chondrocyte recruitment and differentiation. We hypothesized that rhPTH would improve tendon‐to‐bone healing in a rat rotator cuff repair model. One hundred and fourteen Sprague Dawley rats underwent division and repair of the supraspinatus tendon. Fifty seven rats received daily subcutaneous injections of 10 µg/kg of rhPTH. Rats were sacrificed at 3, 7, 14, 28, and 56 days for histologic and immunohistochemical analysis. In addition, rats in each group were sacrificed at 14, 28, and 56 days for biomechanical testing and micro CT analysis. At 2 weeks the controls had a significantly higher load to failure than the rhPTH group. At 28 and 56 days there were no differences in load to failure. rhPTH specimens had significantly higher stiffness at 56 days. MicroCT analysis showed that the rhPTH group had significantly greater total mineral content at all time points, as well as significantly higher bone volume (BV) at 14 and 28 days. Histologically, the rhPTH specimens had more fibrocartilage, osteoblasts, and blood vessels at all timepoints, with significantly better collagen fiber orientation at 28 and 56 days. Although treatment with rhPTH resulted in an increase in bone and mineralized fibrocartilage formation, as well as better collagen fiber organization, this did not translate into improved biomechanical properties. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:769–774, 2012
Special considerations in pediatric gastrointestinal tumors
Authors: Pappo Alberto S. , Janeway Katherine , LaQuaglia Michael , Kim Su Young 
Published: November 2011

AbstractPediatric gastrointestinal tumors are rare in children but are being increasingly recognized. These tumors have distinct biologic and clinical feature that are different from those observed in adults. This review highlights the biological and clinical characteristics of pediatric GIST and provides diagnostic and therapeutic guidelines for the management these unique and challenging group of tumors. J. Surg. Oncol. 2011; 104:928–932. © 2011 Wiley Periodicals, Inc.
Histopathology of gastrointestinal stromal tumor
Authors: Miettinen Markku , Lasota Jerzy 
Published: November 2011

AbstractGastrointestinal stromal tumor (GIST), generally driven by oncogenic KIT or PDGFRA mutations, is the most common mesenchymal tumor of the gastrointestinal (GI) tract. GIST is most common in the stomach (60%) and small intestine (30%), but can occur anywhere in the GI‐tract and the intra‐abdominal soft tissues. GIST can show spindle cell or epithelioid morphology, and mitotic count and tumor size are most important prognostic parameters. GISTs in NF1 patients and children are distinctive clinicopathologic groups. Immunohistochemical testing for KIT and sometimes for DOG1/Ano 1 is essential in confirming the diagnosis. J. Surg. Oncol. 2011; 104:865–873. © 2011 Wiley Periodicals, Inc.
In Situ One‐Step Method for Synthesis of “Click”‐Functionalized Monolithic Stationary Phase for Capillary Electrochromatography
Authors: Salwiński Aleksander , Roy Vincent , Agrofoglio Luigi A. , Delépée Raphaël 
Published: November 2011

AbstractPropargyl acrylate‐based functionalizable monolithic stationary phase (FMSP) for capillary electrochromatography, easily modifiable by alkyne–azide 1,3‐dipolar cycloaddition, is developed. Optimization of FMSP is focused on (i) physical properties: rigidity, stability, and pore size and (ii) the ease of capillary preparation and grafting. The effect of grafting is demonstrated on the separation of eleven polycyclic aromatic hydrocarbons (PAHs), three flavonols, and chlorpheniramine before and after derivatization. Two azides are synthesized: the first, cinnamyl azide increases the retention of PAHs and improves separation of flavonols, the second, 6‐azido‐6‐deoxy‐β‐cyclodextrin allows the chiral separation of warfarin, improves the efficiency of PAHs separation and increases retention of chlorpheniramine.
Calpain 2‐dependent IκBα degradation mediates CPT‐11 secondary resistance in colorectal cancer xenografts
Authors: Fenouille Nina , Grosso Sebastien , Yunchao SU , Mary Didier , Pontier‐Bres Rodolphe , Imbert Véronique , Czerucka Dorota , Caroli‐Bosc François‐Xavier , Peyron Jean‐François , Lagadec Patricia 
Published: November 2011

AbstractCPT‐11 (irinotecan), the first‐line chemotherapy for advanced stage colorectal cancer, remains inactive in about half of patients (primary chemoresistance) and almost all initial responders develop secondary resistance after several courses of treatment (8 months on average). Nude mice bearing HT‐29 colon cancer xenografts were treated with CPT‐11 and/or an NF‐κB inhibitor for two courses. We confirm that NF‐κB inhibition potentiated CPT‐11 anti‐tumoural effect after the first course of treatment. However, tumours grew again at the end of the second course of treatment, generating resistant tumours. We observed an increase in the basal NF‐κB activation in resistant tumours and in two resistant sublines, either obtained from resistant HT‐29 tumours (HT‐29R cells) or generated in vitro (RSN cells). The decrease of NF‐κB activation in HT‐29R and RSN cells by stable transfections with the super‐repressor form of IκBα augmented their sensitivity to CPT‐11. Comparing gene expression profiles of HT‐29 and HT‐29R cells, we identified the S100A10/Annexin A2 complex and calpain 2 as over‐expressed potential NF‐κB inducers. SiRNA silencing of calpain 2 but not of S100A10 and/or annexin A2, resulted in a decrease in NF‐κB activation, an increase in cellular levels of IκBα and a partial restoration of the CPT‐11 sensitivity in both HT‐29R and RSN cells, suggesting that calpain 2‐dependent IκBα degradation mediates CPT‐11 secondary resistance. Thus, targeted therapies directed against calpain 2 may represent a novel strategy to enhance the anti‐cancer efficacy of CPT‐11. Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Assisting children and families with military‐related disruptions: The United States Marine Corps school liaison program
Authors: Aronson Keith R. , Caldwell Linda L. , Perkins Daniel F. , Pasch Kenneth W. 
Published: November 2011

AbstractMilitary families face a number of unique challenges, including frequent relocations and school transitions, as well as extended separations from loved ones. The military, schools, and communities have been working together to build the capacity of children, youth, and families to successfully cope with the stressors they encounter. Most branches of the military have instituted liaison programs within schools and communities to help military school‐aged children make more seamless transitions from one school to another due to relocations. This study assessed the thoughts and perceptions of U.S. Marine Corps (USMC) school liaison professionals (SLs) regarding their work with a broad array of stakeholders, both on‐ and off‐base. Generally, SLs expressed positive sentiments regarding the USMC liaison program. They also reported that they were working hard at developing stronger connections to various constituencies in position to assist military families. Unfortunately, there was some indication that SLs may be at risk for burnout. Given the needs of military children, youth, and families, SLs appear to be filling an important function that could enhance military–school–community partnerships. © 2011 Wiley Periodicals, Inc.
Seven reliability indices for high‐stakes decision making: Description, selection, and simple calculation
Authors: Smith Stacey L. , Vannest Kimberly J. , Davis John L. 
Published: November 2011

AbstractThe reliability of data is a critical issue in decision‐making for practitioners in the school. Percent Agreement and Cohen's kappa are the two most widely reported indices of inter‐rater reliability, however, a recent Monte Carlo study on the reliability of multi‐category scales found other indices to be more trustworthy given the type of data and number of categories. This manuscript presents defensible decision steps, methods, and rationale for selecting and calculating inter‐rater reliability for practitioners. In addition to using screen shots from readily available online programs for calculation such as Excel and Vassar College (Lowry, 2010), decision guides and a flow chart are presented in several figures and tables for easy reference. © 2011 Wiley Periodicals, Inc.
UTP affects the Schwannoma cell line proteome through P2Y receptors leading to cytoskeletal reorganisation
Authors: Martiáñez Tánia , Carrascal Montserrat , Lamarca Aloa , Segura Mònica , Durany Núria , Masgrau Roser , Abian Joaquín , Gella Alejandro 
Published: November 2011

AbstractGlial cells in the peripheral nervous system, such as Schwann cells, respond to nucleotides, which play an important role in axonal regeneration and myelination. Metabotropic P2Y receptor agonists are promising therapeutic molecules for peripheral neuropathies. Nevertheless, the proteomic mechanisms involved in nucleotide action on Schwann cells remain unknown. Here, we studied intracellular protein changes in RT4‐D6P2T Schwann cells after treatment with nucleotides and Nucleo CMP Forte (CMPF), a nucleotide‐based drug. After treatment with CMPF, 2‐D DIGE revealed 11 differential gel spots, which were all upregulated. Among these, six different proteins were identified by MS. Some of these proteins are involved in actin remodelling (actin‐related protein, Arp3), membrane vesicle transport (Rab GDP dissociation inhibitor β, Rab GDI), and the endoplasmic reticulum stress response (protein disulfide isomerase A3, PDI), which are hallmarks of a possible P2Y receptor signalling pathway. Expression of P2Y receptors in RT4‐D6P2T cells was demonstrated by RT‐PCR and a transient elevation of intracellular calcium measured in response to UTP. Actin reorganisation was visualized after UTP treatment using phalloidin–FITC staining and was blocked by the P2Y antagonist suramin, which also inhibited Arp3, Rab GDI, and PDI protein upregulation. Our data indicate that extracellular UTP interacts with Schwann P2Y receptors and activates molecular machinery that induces changes in the glial cell cytoskeleton.
Recommendations for mass spectrometry data quality metrics for open access data (corollary to the Amsterdam principles)
Authors: Kinsinger Christopher R. , Apffel James , Baker Mark , Bian Xiaopeng , Borchers Christoph H. , Bradshaw Ralph , Brusniak Mi‐Youn , Chan Daniel W. , Deutsch Eric W. , Domon Bruno , Gorman Jeff , Grimm Rudolf , Hancock William , Hermjakob Henning , Horn David , Hunter Christie , Kolar Patrik , Kraus Hans‐Joachim , Langen Hanno , Linding Rune , Moritz Robert L. , Omenn Gilbert S. , Orlando Ron , Pandey Akhilesh , Ping Peipei , Rahbar Amir , Rivers Robert , Seymour Sean L. , Simpson Richard J. , Slotta Douglas , Smith Richard D. , Stein Stephen E. , Tabb David L. , Tagle Danilo , Yates John R. , Rodriguez Henry 
Published: November 2011

AbstractPolicies supporting the rapid and open sharing of proteomic data are being implemented by the leading journals in the field. The proteomics community is taking steps to ensure that data are made publicly accessible and are of high quality, a challenging task that requires the development and deployment of methods for measuring and documenting data quality metrics. On September 18, 2010, the U.S. National Cancer Institute (NCI) convened the “International Workshop on Proteomic Data Quality Metrics” in Sydney, Australia, to identify and address issues facing the development and use of such methods for open access proteomics data. The stakeholders at the workshop enumerated the key principles underlying a framework for data quality assessment in mass spectrometry data that will meet the needs of the research community, journals, funding agencies, and data repositories. Attendees discussed and agreed upon two primary needs for the wide use of quality metrics: (i) an evolving list of comprehensive quality metrics and (ii) standards accompanied by software analytics. Attendees stressed the importance of increased education and training programs to promote reliable protocols in proteomics. This workshop report explores the historic precedents, key discussions, and necessary next steps to enhance the quality of open access data. By agreement, this article is published simultaneously in Proteomics, Proteomics Clinical Applications, Journal of Proteome Research, and Molecular and Cellular Proteomics, as a public service to the research community. The peer review process was a coordinated effort conducted by a panel of referees selected by the journals.
Augmented reality and haptic interfaces for robot‐assisted surgery
Authors: Yamamoto Tomonori , Abolhassani Niki , Jung Sung , Okamura Allison M. , Judkins Timothy N. 
Published: November 2011

AbstractBackgroundCurrent teleoperated robot‐assisted minimally invasive surgical systems do not take full advantage of the potential performance enhancements offered by various forms of haptic feedback to the surgeon. Direct and graphical haptic feedback systems can be integrated with vision and robot control systems in order to provide haptic feedback to improve safety and tissue mechanical property identification.MethodsAn interoperable interface for teleoperated robot‐assisted minimally invasive surgery was developed to provide haptic feedback and augmented visual feedback using three‐dimensional (3D) graphical overlays. The software framework consists of control and command software, robot plug‐ins, image processing plug‐ins and 3D surface reconstructions.ResultsThe feasibility of the interface was demonstrated in two tasks performed with artificial tissue: palpation to detect hard lumps and surface tracing, using vision‐based forbidden‐region virtual fixtures to prevent the patient‐side manipulator from entering unwanted regions of the workspace.ConclusionsThe interoperable interface enables fast development and successful implementation of effective haptic feedback methods in teleoperation. Copyright © 2011 John Wiley & Sons, Ltd.
A Critical Requirement for Notch Signaling in Maintenance of the Quiescent Skeletal Muscle Stem Cell State
Authors: Mourikis Philippos , Sambasivan Ramkumar , Castel David , Rocheteau Pierre , Bizzarro Valentina , Tajbakhsh Shahragim 
Published: November 2011

Abstract
Notch signaling plays a key role in virtually all tissues and organs in metazoans; however, limited examples are available for the regulatory role of this pathway in adult quiescent stem cells. We performed a temporal and ontological assessment of effectors of the Notch pathway that indicated highest activity in freshly isolated satellite cells and, unexpectedly, a sharp decline before the first mitosis, and subsequently in proliferating, satellite cell-derived myoblasts. Using genetic tools to conditionally abrogate canonical Notch signaling during homeostasis, we demonstrate that satellite cells differentiate spontaneously and contribute to myofibers, thereby resulting in a severe depletion of the stem cell pool. Furthermore, whereas loss of Rbpj function provokes some satellite cells to proliferate before fusing, strikingly, the majority of mutant cells terminally differentiate unusually from the quiescent state, without passing through S-phase. This study establishes Notch signaling pathway as the first regulator of cellular quiescence in adult muscle stem cells.
Disclosure of potential conflicts of interest is found at the end of this article.
An automatic VHF transmitter monitoring system for wildlife research
Authors: Johansson A. Torbjörn , Johansson Örjan , McCarthy Tom 
Published: November 2011

AbstractWe describe an automated system for monitoring multiple very high frequency (VHF) transmitters, which are commonly employed in wildlife studies. The system consists of a microprocessor‐controlled radio‐frequency monitor equipped with advanced signal‐processing capabilities that communicates with, and relays information to, a user interface unit at a different location. The system was designed for a capture‐and‐release snow leopard (Panthera uncia) study in Mongolia, where checking trap‐site transmitters manually entailed climbing a hill with telemetry equipment several times each day and night. Here, it monitors the trap‐site transmitters and actively produces an alarm when any of the traps have been triggered, or if the system has lost contact with any trap‐transmitter. The automated system allowed us to constantly monitor transmitters from a research camp, and alerted us each time a trap was triggered. The system has been field‐tested for 83 days from mid‐September 2010 to mid‐December 2010 in the Tost mountain range on the edge of Mongolia's Gobi desert. During this time, the system performed reliably, responding correctly to 45 manually generated alarms and 9 animal captures. The system considerably shortens the time the captured animals spend in traps, and also mitigates the need for manual trap‐site transmitter monitoring, greatly reducing risk to the animal and the human effort involved. © 2011 The Wildlife Society.
Natal movement in juvenile Atlantic salmon: a body size‐dependent strategy?
Authors: Einum Sigurd , Finstad Anders G. , Robertsen Grethe , Nislow Keith H. , McKelvey Simon , Armstrong John D. 
Published: November 2011

AbstractIf competitive ability depends on body size, then the optimal natal movement from areas of high local population density can also be predicted to be size‐dependent. Specifically, small, competitively‐inferior individuals would be expected to benefit most from moving to areas of lower local density. Here we evaluate whether individual variation in natal movement following emergence from nests is consistent with such a size‐dependent strategy in Atlantic salmon, and whether such a strategy is evident across a range of environmental conditions (principally predator presence and conspecific density). In stream channel experiments, those juveniles that stayed close to nests were larger than those that emigrated. This result was not sensitive to predator presence or conspecific density. These observations were mirrored in natural streams in which salmon eggs were planted in nests and the resulting offspring were sampled at high spatial resolution. A negative relationship was found between juvenile body size and distance from nests early in development whereas in those streams sampled later in ontogeny, individuals that had moved furthest were largest. Thus, movement away from nests appeared to result in a reduced competitive intensity and increased growth rate. The fact that there is ultimately a growth advantage associated with moving suggests that there is also a cost that selects against movement by the larger individuals. Thus, natal movement in juvenile Atlantic salmon appears to represent a body size‐dependent strategy.
Head Trauma in the Cat: 1. Assessment and Management of Craniofacial Injury
Authors: Adamantos Sophie , Garosi Laurent 
Published: November 2011

Practical relevance Feline trauma is commonly seen in general practice and frequently involves damage to the head. Clinical challenges While craniofacial injuries following trauma vary widely in severity, affected cats can often be severely compromised in terms of their neurological, respiratory and cardiovascular status, and their management can be challenging in both the short and long term. They need prompt stabilisation and careful monitoring in the initial period to maximise prospects of a successful outcome. Many cats with severe facial trauma will require surgery to stabilise skull fractures or address injuries to the eyes, with its inherent issues surrounding pain management, ensuring adequate nutrition and the necessity for ongoing hospitalisation. Diagnostics Cats with head trauma benefit from imaging of the injured areas as well as thoracic radiography. Imaging the skull can be challenging and is best performed under general anaesthesia. In unstable patients this can be delayed to prevent any associated morbidity. Evidence base The clinical evidence base relating to injury to the feline head is limited, despite its relative frequency in general practice. This review focuses on the initial approach to craniofacial (in particular, ocular and jaw) trauma, and outlines simple techniques for management of soft tissue and bone injuries. Much of the information is based on the authors' clinical experience, as there is a paucity of well-described clinical case material.
The Text of the De Virginitate of Athanasius
Authors: Lake Kirsopp , Casey Robert P. 
Published: November 2011

The edition of the “De Virginitate” of Athanasius by Eduard von der Goltz (Texte und Untersuchungen xxix, 1905) is one of the most important contributions made in modern times to the knowledge of the text of Athanasius, because of its full discussion of the variant readings of the manuscripts. The work, however, was handicapped by incomplete knowledge of the Patmos MS. (P), which von der Goltz regarded as the best, and by almost complete ignorance of the two Vatopedi MSS. quoted by him under the symbols W1 and W2, but in the present article designated A and K, since that nomenclature was adopted in the first article on their contents in the Journal of Theological Studies, vol. v, 1903, pp. 108 ff.A visit to Athos and Patmos in 1925 has made it possible to build a little further on von der Goltz's foundation, and to correct some of the errors inevitable to the conditions under which he worked. We shall here give:(I) A fuller account of P.(II) A list of corrections to be made in von der Goltz's collation of P.(III) A collation, with von der Goltz's edition, of the text of the two Vatopedi MSS. A and K, and of a third MS. in the same library, which we call Q.(IV) Some discussion of the general problem of the text of the “De Virginitate.”
An Early Papyrus Fragment of the Gospel of Matthew in the Michigan Collection
Authors: Sanders Henry A. 
Published: November 2011

Greek Papyrus 1570 of the University of Michigan Collection is a leaf of a papyrus book written on both sides. The preserved portion measures 4¾ by 9 inches (121 by 224 mm.). One entire line is lost at the bottom, and over half of each of the four lines immediately preceding. Of the top line only eight or nine letters are preserved. All other lines are somewhat damaged at one end or the other. Such loss often amounts to five or six letters. Eight lines in the middle of the leaf are damaged at both ends. Originally the size of the leaf must have been about 6 by 10 inches. There were 33 lines, which varied from 40 to 50 letters in length. The text covered is Matthew 26, 19 συνέταξεν to 26, 52 ἀπολοῦνται.So large a papyrus fragment of Matthew's version of the Lord's last supper and betrayal is certain to be of special importance, as well for its content as for the bearing it has on the problem of the New Testament text.