bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ecological aspects and relationships of the emblematic Vachellia spp. exposed to anthropic pressures and parasitism in natural hyper-arid ecosystems: ethnobotanical elements, morphology, and biological nitrogen fixation
Authors: Vincent Bryan , Bourillon Julie , Gotty Karine , Boukcim Hassan , Selosse Marc-André , Cambou Aurélie , Damasio Coraline , Voisin Mathis , Boivin Stéphane , Figura Tomas , Nespoulous Jérôme , Galiana Antoine , Maurice Kenji , Ducousso Marc 
Published: April 2024

Abstract
Main conclusion
Emblematic Vachellia spp. naturally exposed to hyper-arid conditions, intensive grazing, and parasitism maintain a high nitrogen content and functional mutualistic nitrogen-fixing symbioses.

Abstract
AlUla region in Saudi Arabia has a rich history regarding mankind, local wildlife, and fertility islands suitable for leguminous species, such as the emblematic Vachellia spp. desert trees. In this region, we investigated the characteristics of desert legumes in two nature reserves (Sharaan and Madakhil), at one archaeological site (Hegra), and in open public domains at Al. Ward and Jabal Abu Oud. Biological nitrogen fixation (BNF), isotopes, and N and C contents were investigated through multiple lenses, including parasitism, plant tissues, species identification, plant maturity, health status, and plant growth. The average BNF rates of 19 Vachellia gerrardii and 21 Vachellia tortilis trees were respectively 39 and 67%, with low signs of inner N content fluctuations (2.10–2.63% N) compared to other co-occurring plants. The BNF of 23 R. raetam was just as high, with an average of 65% and steady inner N contents of 2.25 ± 0.30%. Regarding parasitism, infected Vachellia trees were unfazed compared to uninfected trees, thereby challenging the commonly accepted detrimental role of parasites. Overall, these results suggest that Vachellia trees and R. raetam shrubs exploit BNF in hyper-arid environments to maintain a high N content when exposed to parasitism and grazing. These findings underline the pivotal role of plant-bacteria mutualistic symbioses in desert environments. All ecological traits and relationships mentioned are further arguments in favor of these legumes serving as keystone species for ecological restoration and agro-silvo-pastoralism in the AlUla region.

Graphical abstract
Effect of antioxidant-rich kindergarten meals on oxidative stress biomarkers in healthy 5–6-year-old children: a randomized controlled trial
Authors: Berlic Maja , Korošec Mojca , Remec Žiga Iztok , Čuk Vanja , Battelino Tadej , Repič Lampret Barbka 
Published: April 2024

AbstractAs children spend up to 9 h a day in kindergarten, the main purpose of our study was to evaluate the effect of antioxidant-rich kindergarten meals on oxidative stress biomarkers (OSBs) in healthy children. In the randomized control trial with a follow-up, healthy 5–6-year-old children from six kindergartens were randomly divided into a prototype group (PG, n = 40) and a control group (CG, n = 17). PG followed a 2-week antioxidant-rich kindergarten meal plan (breakfast, lunch, and two snacks), and CG followed their standard kindergarten meal plans. Outside the kindergartens, participants ate as usual. We used a consecutive 7-day dietary record inside and outside the kindergarten and the national dietary assessment tool OPEN to assess the total dietary antioxidant capacity (dTAC) of the consumed foods. Malondialdehyde (MDA), 8-hydroxy-2-deoxyguanosine (8-OHdG), and four F2-isoprostane were measured in fasting urine on days 1 and 15. We also measured total antioxidant power (PAT) and hydroperoxides (d-ROMs) in fasting serum on day 15 and obtained the value of the oxidative stress index (OSI). We used a Welch two-sample t-test and multiple regression analysis to compare the prototype and control groups and a nonparametric Wilcoxon signed rank exact test to compare pre- and post-intervention results in urine. Antioxidant-rich kindergarten meals contributed to a significantly (p < 0.05) higher intake of dTAC in PG participants compared to standard meals in CG participants (8.6 vs. 2.8 mmol/day). We detected a negative correlation between dTAC intake and d-ROMs and between dTAC intake and OSI (r =  − 0.29, p = 0.043 and r =  − 0.31, p = 0.032, respectively). A significant decrease in urinary 8-iso-15-prostaglandin-F-2 alpha was detected in PG participants between days 1 and 15; however, no other intra-individual significant differences in urinary OSBs were found. Conclusion: Antioxidant-rich food in kindergarten is warranted due to its potential health-protective effect. Additionally, we present original data on the average levels of urinary and serum OSBs in healthy 5–6-year-old children. Trial registration: The study was registered at ClinicalTrials.gov, on February 5, 2020 (https://clinicaltrials.gov/ct2/show/NCT04252105).

What is Known:• Kindergartens are recognized as promising environments for public health measures.• A diet rich in antioxidants can reduce OSBs and, consequently, the risk of developing NCDs.

What is New:• Antioxidant-rich kindergarten diet can ensure a protective intake of dTAC in children.• Original data on serum oxidative stress biomarkers (d-ROMs, PAT, and OSI) and urinary oxidative stress biomarkers (MDA, 8-OHdG, and F2 isoprostanes) in healthy 5–6-year-old children.
CircCOL1A1 promotes proliferation, migration, and invasion of colorectal cancer (CRC) cells and glutamine metabolism through GLS1 up-regulation by sponging miR-214-3p
Authors: Liu Jia , Zhang Xianbo , Yang Meijian , Zhang Xianghong 
Published: April 2024

Abstract
Background
Circular ribose nucleic acids (circRNAs), an abundant type of noncoding RNAs, are widely expressed in eukaryotic cells and exert a significant impact on the initiation and progression of various disorders, including different types of cancer. However, the specific role of various circRNAs in colorectal cancer (CRC) pathology is still not fully understood.

Methods
The initial step involved the use of quantitative reverse transcription polymerase chain reaction (RT-qPCR) to assess the expression levels of circRNAs and messenger RNA (mRNA) in CRC cell lines and tissues. Subsequently, functional analyses of circCOL1A1 knockdown were conducted in vitro and in vivo through cell counting kit (CCK)-8, colony formation and transwell assays, as well as xenograft mouse model of tumor formation. Molecular expression and interactions were investigated using luciferase reporter assays, Western blot analysis, RNA immunoprecipitation (RIP), and immunohistochemical staining.

Results
The RT-qPCR results revealed elevated levels of circCOL1A1 expressions in CRC tissues and cell lines as compared to the normal counterparts. In addition, circCOL1A1 expression level was found to be correlated with TNM stage, lymph node metastases, distant metastases, and invasion. Knockdown of circCOL1A1 resulted in impaired invasion, migration, and proliferation of CRC cells, and suppressed tumor generation in the animal model. We further demonstrated that circCOL1A1 could act as a sponge for miR-214-3p, suppressing miR-214-3p activity and leading to the upregulation of GLS1 protein to promote glutamine metabolism.

Conclusion
These findings suggest that circCOL1A1 functions as an oncogenic molecule to promote CRC progression via miR-214-3p/GLS1 axis, hinting on the potential of circCOL1A1 as a therapeutic target for CRC.
Seeds in the guts: can seed traits explain seed survival after being digested by wild ungulates?
Authors: Lepková Barbora , Mašková Tereza 
Published: April 2024

AbstractPlants inhabiting open landscapes are often dispersed by ungulates and are expected to be adapted to this type of dispersal through their seed traits. To find which traits help seeds survive the passage through digestion of wild ungulates, we conducted a comprehensive feeding experiment with almost forty species of plants and three species of ungulates. We fed specified numbers of seeds to the animals, collected the dung, and germinated the dung content. We explored whether seed morphological traits and seed nutrient contents are good predictors of seed survival after passage through the ungulate digestive system. We also tested how the seed survival differed after the passage through different ungulate species. To find answers, we used GLMM with beta-binomial distribution and animal and plant species as random factor, respectively. We found that species survival and germination success were negatively correlated to seed elongation and the thickness of the seed coat. Even though phylogenetically correct GLMM did not yield significant results, when we tested species from commonly represented families, separately (legumes and grasses compared to all other species) different traits had statistically significant effects. In the case of seed elongation, the effect changed direction from negative to positive when legumes and grasses were left out. Our results suggest that seed traits enabling species survival after passage through the digestive tract are strongly phylogenetically conserved and different groups of plants evolved different ways of adapting to grazing pressure and utilize it for dispersal.
Modelling and simulation of anisotropic growth in brain tumours through poroelasticity: A study of ventricular compression and therapeutic protocols
Authors: Ballatore Francesca , Lucci Giulio , Giverso Chiara 
Published: April 2024

AbstractMalignant brain tumours represent a significant medical challenge due to their aggressive nature and unpredictable locations. The growth of a brain tumour can result in a mass effect, causing compression and displacement of the surrounding healthy brain tissue and possibly leading to severe neurological complications. In this paper, we propose a multiphase mechanical model for brain tumour growth that quantifies deformations and solid stresses caused by the expanding tumour mass and incorporates anisotropic growth influenced by brain fibres. We employ a sharp interface model to simulate localised, non-invasive solid brain tumours, which are those responsible for substantial mechanical impact on the surrounding healthy tissue. By using patient-specific imaging data, we create realistic three-dimensional brain geometries and accurately represent ventricular shapes, to evaluate how the growing mass may compress and deform the cerebral ventricles. Another relevant feature of our model is the ability to simulate therapeutic protocols, facilitating the evaluation of treatment efficacy and guiding the development of personalized therapies for individual patients. Overall, our model allows to make a step towards a deeper analysis of the complex interactions between brain tumours and their environment, with a particular focus on the impact of a growing cancer on healthy tissue, ventricular compression, and therapeutic treatment.
Comparing symptom clusters in cancer survivors by cancer diagnosis: A latent class profile analysis
Authors: Lee Lena J. , Han Claire J. , Saligan Leorey , Wallen Gwenyth R. 
Published: April 2024

Abstract
Purpose
Research on symptom clusters in oncology is progressing, but knowledge gaps remain. One question is whether the number and types of symptom subgroups (i.e., latent classes) differ based on cancer diagnosis. The purpose of this study was to: (1) identify and compare latent class subgroups based on four highly prevalent symptoms (pain, fatigue, sleep disturbance, and depression), and (2) examine the differences in sociodemographic and clinical factors in the identified latent classes across the seven cancer types (i.e., prostate, non-small cell lung, non-Hodgkin’s lymphoma, breast, uterine, cervical, and colorectal cancer).

Methods
This study is a cross-sectional secondary analysis of data obtained from the My-Health study in partnership with four Surveillance, Epidemiology, and End Results (SEER) cancer registries located in California (two), Louisiana, and New Jersey. The sample included 4,762 cancer survivors 6-13 months following diagnosis of one of the seven cancer types mentioned. Latent class profile analysis was used.

Results
Subjects were primarily young (59% age 21-64 years), Caucasian (41%), married/cohabitating (58%) and unemployed (55%). The number and types of symptom subgroups varied across these seven cancer populations: four-subgroups were the common in prostate, lung, non-Hodgkin’s lymphoma, and breast cancer survivors. Unmarried, low education, and unemployment status were associated with high risk of symptom burden across the cancer types.

Conclusion
Identifying symptom subgroups by cancer diagnosis has the potential to develop innovative and effective targeted interventions in cancer survivors. Further research is needed to establish extensive knowledge in symptom clustering between treatment regimens, and short-term and long-term cancer survivors.
DWT-BILSTM-based models for day-ahead hourly global horizontal solar irradiance forecasting
Authors: Çevik Bektaş Sibel , Altaş Ismail H. 
Published: April 2024

AbstractAccurate forecasting of electricity generation from renewable energy sources is crucial for the operation, planning and management of smart grids. For reliable planning and operation of photovoltaic (PV) systems in grid-connected or islanded utilities, an hourly day-ahead forecast of PV output is critical. The forecast of PV power can be done indirectly by estimating solar irradiance. For forecasting day-ahead hourly global horizontal irradiance (GHI), two forecasting models with different multivariate inputs are proposed in this paper, and the results are compared. These models use a hybrid algorithm of discrete wavelet decomposition and bidirectional long short-term memory (BILSTM). The inputs of the first model contain GHI and weather type data. The other model allows for observation of the effect of meteorological values including GHI, temperature, humidity, wind speed, and weather type data. The forecasting performance of deep learning algorithms which contain recurrent neural network (RNN), long short-term memory (LSTM), and BILSTM algorithms for day ahead hourly solar irradiance forecasting problems are also compared. To evaluate the performance of proposed models, two datasets are used for Model 1 and one dataset is used for Model 2. An experiment is also done to demonstrate that the proposed Model 1 is applicable in datasets collected in the vicinity of the city of Trabzon. On the other hand, BILSTM algorithm outperforms RNN and LSTM algorithms. It is seen that the test successes of both proposed models are better than the results given in the literature.
Predictors of failure after primary anterior cervical discectomy and fusion for subaxial traumatic spine injuries
Authors: Singh Aman , El-Hajj Victor Gabriel , Fletcher-Sandersjöö Alexander , Aziz Nabeel , Ghaith Abdul Karim , Tatter Charles , Blixt Simon , Nilsson Gunnar , Bydon Mohamad , Gerdhem Paul , Edström Erik , Elmi-Terander Adrian 
Published: April 2024

Abstract
Introduction
Traumatic subaxial fractures account for more than half of all cervical spine injuries. The optimal surgical approach is a matter of debate and may include anterior, posterior or a combined anteroposterior (360º) approach. Analyzing a cohort of patients initially treated with anterior cervical discectomy and fusion (ACDF) for traumatic subaxial injuries, the study aimed to identify predictors for treatment failure and the subsequent need for supplementary posterior fusion (PF).

Methods
A retrospective, single center, consecutive cohort study of all adult patients undergoing primary ACDF for traumatic subaxial cervical spine fractures between 2006 and 2018 was undertaken and 341 patients were included. Baseline clinical and radiological data for all included patients were analyzed and 11 cases of supplementary posterior fixation were identified.

Results
Patients were operated at a median of 2.0 days from the trauma, undergoing 1-level (78%), 2-levels (16%) and ≥ 3-levels (6.2%) ACDF. A delayed supplementary PF was performed in 11 cases, due to ACDF failure. On univariable regression analysis, older age (p = 0.017), shorter stature (p = 0.031), posterior longitudinal ligament (PLL) injury (p = 0.004), injury to ligamentum flavum (p = 0.005), bilateral facet joint dislocation (p < 0.001) and traumatic cervical spondylolisthesis (p = 0.003) predicted ACDF failure. On the multivariable regression model, older age (p = 0.015), PLL injury (p = 0.048), and bilateral facet joint dislocation (p = 0.010) remained as independent predictors of ACDF failure.

Conclusions
ACDF is safe and effective for the treatment of subaxial cervical spine fractures. High age, bilateral facet joint dislocation and traumatic PLL disruption are independent predictors of failure. We suggest increased vigilance regarding these cases.
Extended reality in cranial and spinal neurosurgery – a bibliometric analysis
Authors: Buwaider Ali , El-Hajj Victor Gabriel , Mahdi Omar Ali , Iop Alessandro , Gharios Maria , de Giorgio Andrea , Romero Mario , Gerdhem Paul , Jean Walter C , Edström Erik , Elmi-Terander Adrian 
Published: April 2024

Abstract

Purpose
This bibliometric analysis of the top 100 cited articles on extended reality (XR) in neurosurgery aimed to reveal trends in this research field. Gender differences in authorship and global distribution of the most-cited articles were also addressed.

Methods
A Web of Science electronic database search was conducted. The top 100 most-cited articles related to the scope of this review were retrieved and analyzed for trends in publications, journal characteristics, authorship, global distribution, study design, and focus areas. After a brief description of the top 100 publications, a comparative analysis between spinal and cranial publications was performed.

Results
From 2005, there was a significant increase in spinal neurosurgery publications with a focus on pedicle screw placement. Most articles were original research studies, with an emphasis on augmented reality (AR). In cranial neurosurgery, there was no notable increase in publications. There was an increase in studies assessing both AR and virtual reality (VR) research, with a notable emphasis on VR compared to AR. Education, surgical skills assessment, and surgical planning were more common themes in cranial studies compared to spinal studies. Female authorship was notably low in both groups, with no significant increase over time. The USA and Canada contributed most of the publications in the research field.

Conclusions
Research regarding the use of XR in neurosurgery increased significantly from 2005. Cranial research focused on VR and resident education while spinal research focused on AR and neuronavigation. Female authorship was underrepresented. North America provides most of the high-impact research in this area.
Effects of different primers and colouring solutions on orthodontic bonding: shear bond strength and colour change
Authors: Eren Hatice Kübra , Bilgiç Zortuk Fundagül 
Published: April 2024

Abstract
Objectives
This in vitro study evaluated the effect of different colouring solutions and primer systems used in the bonding of brackets on enamel colour change and bond strength.

Materials and methods
120 premolar teeth were divided into four main groups; brackets were bonded with 37% orthophosphoric acid + Transbond XT Primer in Group 1, 3 M Single Bond Universal in Group 2, Transbond Plus SEP in Group 3, and G-Premio Bond in Group 4. Each group was divided into three subgroups, and the teeth were placed in a cup containing coffee and tea mixture, in a cup containing cola and in distilled water. A bond strength test was applied to all teeth. Colour measurements of all teeth were performed at 2 different times: before bonding and after the bond strength test.

Results
The average bond strength of the 37% orthophosphoric acid group was higher than that of the other groups. The effect of primer and solution groups on colour change was statistically significant (p = 0.001 and p = 0.023, respectively).

Conclusions
In this study, the bond strength was clinically sufficient in all primer groups. The highest colour change was observed when the tea–coffee solution and Transbond Plus SEP primer were used.

Clinical relevance
This study has identified enamel discoloration and bond strength from different colouring solutions and primer systems used for bonding braces, which can be used to inform clinicians and patients to achieve better treatment results.
Randomized controlled clinical trial on the efficacy of a novel antimicrobial chewing gum in reducing plaque and gingivitis in adolescent orthodontic patients
Authors: Weber Johanna , Scholz Konstantin J. , Schenke Isabelle M. , Pfab Florian , Cieplik Fabian , Hiller Karl-Anton , Buchalla Wolfgang , Sahm Camilla , Kirschneck Christian , Paddenberg-Schubert Eva 
Published: April 2024

Abstract
Objectives
Chewing gums containing antiseptics or other antimicrobial substances may be effective in reducing plaque and gingivitis. Therefore, the aim of this randomized placebo-controlled clinical trial was to investigate the efficacy of a novel antimicrobial chewing gum containing essential oils (cinnamon, lemon, peppermint) and extracts on reduction of dental plaque and gingivitis as well as on oral health-related quality of life (OHRQoL) in adolescent orthodontic patients.

Materials
52 patients (11-22 years of age) were randomly assigned to use a test chewing gum (COVIDGUM, Clevergum) or a commercially available control chewing gum over a period of 10 days. Approximal plaque index (API), papillary bleeding index (PBI) and an OHRQoL questionnaire for children (COHIP-G19) were assessed at baseline (BL), after 10 days (10d) and 30 days (30d). In addition, oral health and oral hygiene related questions of the COHIP-G19 questionnaire were evaluated separately in subscales at each timepoint. Data were analyzed using non-parametrical statistical procedures (α = 0.05).

Results
API and PBI decreased significantly over time from BL to 10d and from BL to 30d in both groups, without significant differences between the groups. In both groups, the COHIP-G19 score, oral health subscale and oral hygiene subscale decreased significantly over time. Regarding the oral hygiene subscale, the test group showed significantly better scores at 30d (p = 0.011).

Conclusion
Both chewing gums performed similarly effective in terms of reducing plaque accumulation and gingival inflammation and improving OHRQoL.

Clinical relevance
Chewing gums without antimicrobial ingredients may be sufficient to decrease plaque accumulation and gingival inflammation.
Template-assisted electrodeposition of freestanding antimony, tin, and antimony-tin nanowire arrays from an ionic liquid
Authors: Al Kiey Sherief A. , Farag H. K. , Abedin S. Zein El 
Published: April 2024

AbstractIn the present study, we show the electrochemical synthesis of Sb, Sn, and Sb-Sn nanowire arrays from the ionic liquid 1-butyl-1-methylpyrrolidinium bis(trifluoromethylsulfonate ([Py1,4]TfO) via templated-assisted approaches. Commercially available track etched polycarbonate template with a nominal pore diameter of 400 nm was utilized as a template. The nanowires were electrochemically deposited inside the pores of the template; then, a supporting copper layer was electrodeposited on the back side of the template. Subsequently, the template was dissolved with dichloromethane, and the structural morphology of the nanowire structures was explored by high-resolution scanning electron microscopy (SEM) and energy dispersive x-ray (EDX). Freestanding, mechanically stable nanowire arrays of Sb, Sn, and Sb-Sn with an average pore diameter of 400 nm were obtained. The charge/discharge characteristics of the electrodeposited nanowire films were investigated to explore the Li storage capacity of the fabricated electrodes. The results revealed that the electrodeposited nanowire films are promising anode candidates for the future generation of Li-ion batteries.
Graphical abstract