bluuebunny/crossref_metadata_2025_split huggingface dataset:
Formulation and Evaluation of Bivalent Swine and Avian Lipid Nanoparticles mRNA Vaccine
Authors: Kassab Mohammed Mahmoud Shawky 
Published: June 2024

المستخلص
تصنيع لقاح مكون من الحمض النووي الريبوزي الرسول الخاص بالبروتينات المسئولة عن انتاج البروتينات نيورامنيديز والهيم اجليوتونين لفيروسي الانفلونزا الخنازير والطيورمحاط بجزيئات دهنية بحجم النانوميتر

عدوى انفلونزا الخنازير وانفلونزا الطيور مشكلة خطيرة تؤرق العالم كله هذه الايام خصوصا
انها مميتة لكثير من اطفال العالم . بعد فترة التعافي من الاصابة تتكون المناعة المكتسبة ضدها
لمدة عام تقريبا.
هذه الانفلونزا تسبب تسمم فيروسي في الدم بالاضافة للالتهاب الرئوي
وهذامن بين اكبرالاسباب للوفاة بين الاطفال قبل سن عامين عالميا بشكل مباشر.
الهدف من الدراسة هو انتاج لقاح الحمض النووي الريبوزي الرسول
من نوع الام بالنانوتكنولوجي لبروتينات الهيم اجليوتونين والنيورامنيديز الموجودة على سطح
هذه الفيروسات التي تساعدتها في الاتصاق بالعائل الذي ستتكاثر داخل خلاياه بالجهاز التنفسي.
هذه الدراسة دراسة استكشافية ومن خلالها
استطاعنا تصميم هذا اللقاح باستعمال نظام توصيل للقاح بالنانوتكنولوجي حجم جزيئاته
70نانوميمتر. اللقاح الجديد اظهر فاعلية بنسبة 60% واظهر فاعلية حيوية مقاربة واثار جانبية
اقل بالمقارنة باللقاحات المتوفرة حاليا بالسوق الدوائي ضد هذا النوع من الفيروسات الوبائية.

ABSTRACT
Background:
Worldwide, Swine and Avian influenza, or the H1N1 and H5N1 serotypes of the Influenza A virus, is a highly contagious illness. In recent years, the global incidence of mortality rate has surpassed 284,000 fatalities.
Aim of the study:
The production of mRNA vaccine lipid nanoparticles (LNP) to combat the deadly strains of Avian and Swine influenza (H1N1 and H5N1 of the influenza A serotype).
Methodology:
Polymerase chain reaction (PCR) was used to clone genes of interest encoding Haemagglutinin (HA) and Neuraminidase (NA) of Influenza A virus strains (H5N1 and H1N1) known as Avian flu and Swine flu, respectively. The genes were then inserted using the EcoR1, BAM Hind II restriction endonuclease type II, and Ligase enzyme into the pET-21(+) transcription vector plasmid. On the other hand, the hybrid transcription vectors were mixed with T7 RNA polymerase and ribonucleotides within a test tube after being linearized using EcoR II. Following their purification via reversed-phase high-performance liquid chromatography (RP-HPLC), the mRNAs of the HA and NA were encased in 70 nm LNPs and subjected to reverse-phase high-performance liquid chromatography (RP-HPLC). Lastly, immunogenicity studies were performed in stages 1/2 of randomized human and preclinical clinical trials.
Results:
The bivalent vaccination had around 63% efficacy in preclinical studies and approximately 60% efficacy in randomized human clinical trials. As shown by the ELISA test, strong neutralizing antibodies against HA were prevalent.
Conclusion:
The current study is a promising effort because of the development of the novel LNP-mRNA vaccine, which could lower the incidence of Avian and Swine flu infectious disorders.
Optimized Smart Home Temperature Monitoring Using IoT
Authors: Putra Dandi Pratama , Ayuni Shazana Dhiya 
Published: June 2024

This research aims to monitor residential home temperatures using the Internet of Things (IoT) and Google Sheets. By adopting a Research and Development (R&D) approach, the study designs and tests a temperature monitoring tool with DHT11 sensors, NodeMCU ESP8266, and LCD displays. Data is integrated into Google Sheets for real-time access. The results show improved service efficiency, reducing waiting times to 0.41-2.45 minutes, with fewer waiting customers and lower utilization rates. This system enhances living conditions by providing easy temperature monitoring, recommending future studies to extend data collection periods for comprehensive analysis. Highlight: Efficient Monitoring: Real-time temperature data via Google Sheets. Integrated System: Uses DHT11 sensors and NodeMCU ESP8266. Research Implications: Extend data collection for thorough analysis. Keywoard: Temperature Monitoring, Internet of Things, Google Sheets, DHT11 Sensors, NodeMCU ESP8266
Management of Traumatic Dental Injuries among Otolaryngologists in Southwestern Mainland China: a cross-sectional observational survey
Authors: Pan Zhongjing , Zeng Qinghan , Ma Tianpei , Peng Yuanyuan , Zhang Jinyi , Xu Jue , Lu Dan 
Published: June 2024

Abstract

Background
This study aimed to examine otolaryngologists' attitudes and knowledge regarding the management of traumatic dental injuries (TDIs) encountered when patients present at the emergency department or during procedures such as tonsillectomy,adenoidectomy and suspension laryngoscopic surgery, as well as their motivation for pursuing additional education in this area.
Methods
A cross-sectional observational survey involving otolaryngologists selected from multiple hospitals across Sichuan Province was carried out in this study. The questionnaire, consisting of 31 questions, was segmented into three sections: demographic data (10 items), knowledge about the management of TDI (17 items), and attitudes toward acquiring dental trauma education (4 items). Descriptive analysis, the Kruskal–Wallis test, the Mann–Whitney U test and ANOVA were used for statistical analysis.
Results
In the analysis, a total of 214 surveys were included, with over half of the participants (60.3%) having experienced at least one TDI case in their practice, while four in ten (39.7%) reported never encountering any cases. Participants who had a longer duration of practice were more likely to have a large caseload on the TDI (χ2 = 29.44, p < 0.001). Seventy-one percent of participants experienced TDI during the perioperative period; however, only 6/214 participants (2.8%) responded correctly to all three questions about emergency management of TDI, and nearly all participants (98.1%) chose to refer TDI patients to a dentist. The majority of participants (91.1%) stated that they had not received any training on TDI, and 94.4% were willing to receive TDI-related education.
Conclusions
The findings of this study highlight the inadequate knowledge of otolaryngologists in southwestern mainland China regarding the management of TDI. Furthermore, the results indicate a strong interest among otolaryngologists in pursuing further training, which could help meet the educational needs necessary for providing appropriate management of TDI patients. Hence, it is imperative to provide targeted training courses to ensure prompt and appropriate TDI treatment, thereby minimizing the associated medical and societal costs.
Application of Low Density Polyethylene (LDPE) Passive Samplers for Monitoring PAHs in Groundwater
Authors: Ola Ibukun , Drebenstedt Carsten , Burgess Robert M. , Allan Ian J. , Hoth Nils , Külls Christoph 
Published: June 2024

Abstract

Equilibrium passive sampling continues to find increasing use for performing in-situ assessments and monitoring of hydrophobic organic compounds (HOCs). Although this method has been successfully used in several field studies including open surface waters and sediments, comparatively, their use in groundwater has been very limited. In this study, low density polyethylene (LDPE) passive samplers were deployed for 80 d in three groundwater wells contaminated with polycyclic aromatic hydrocarbons (PAHs). Prior to deployment, LDPE was loaded with performance reference compounds (PRCs) consisting of deuterated PAHs and their release used to ascertain system equilibrium. Within the 80-d deployment period, LDPE-groundwater equilibrium was confirmed for PAHs with molecular weights (MW) in the range of 178 to 228 (i.e., anthracene, chrysene). Measured freely dissolved concentrations (Cw) were between one to three orders of magnitude lower than the total filtered concentrations (Ctotal) in the studied wells. The sum of PAHs (ΣPAHs) measured based on Cw and Ctotal were 2.05, 0.07, and 29.2 μg L-1 and 197, 59.7 and 1010 μg L-1, at wells 1, 2, and 3, respectively. A separate dataset, comprising long-term (2010 to 2022) concentrations of PAHs in total (i.e., unfiltered) groundwater is also presented to provide insight into PAH contamination levels at the assessed groundwater wells based on conventional measurement. Estimated in-situ LDPE daily clearance volumes (2.34 to 27.56 Ld-1) for the target analytes were far less than the daily turnover of ground water (144 to 348 Ld-1) encountered in the wells eliminating the possibility of depletive sampling of the groundwater by the passive samplers. These results represent the first published study on the practical application of equilibrium passive sampling using LDPE for monitoring and quantitatively assessing PAHs in groundwater. Also, this work demonstrates, LDPE are a useful tool for measuring the Cw of PAHs in groundwater, a critical contaminant in many ecological and human health risk assessments.
Ribes nigrum leaf extract: antioxidant capacity and redox balance regulation mechanism in microglial cells
Authors: Minasyan Alvard , Pires Vivien , Gondcaille Catherine , Savary Stéphane , Cherkaoui-Malki Mustapha , Andreoletti Pierre , Sahakyan Naira 
Published: June 2024

Abstract

This study focuses on the investigation of the antioxidant and anti-inflammatory activity of extracts from Ribes nigrum leaves on BV-2 microglial Wt and Acyl-CoA oxidase 1 deficient (Acox1−/−) cell line models, useful for the investigation of some neurodegenerative disorders. Investigations showed the presence of high quantity of phenolic compounds in this extract, among which flavan-3-ols, flavonols, furanocoumarins, hydroxycinnamates, quercetin, and quercetin derivatives were major components, which are known for their biological activity in various test systems. The MTT test revealed the 0.125 mg/mL concentration of R. nigrum extract as the highest non-toxic. The investigated extract showed high antioxidant activity in chemical-based tests. The antioxidant potential of the R. nigrum leaf extract was furtherly explored using the BV-2 microglial cell line models. Moreover, the extract was found to alter the activity of the main antioxidant enzyme, catalase and fatty acid oxidation enzyme, Acyl-CoA oxidase 1 (ACOX1) as well as the expression of appropriate genes in Wt and Acox1−/− BV-2 microglial cells such as Cat, iNos, Il-1β, Tnf-α, and Abcd1. In Wt cells, the treatment with R. nigrum leaf extract ACOX1 activity was downregulated after 24 hours of treatment, meanwhile the catalase activity remains unchanged. The further treatment leaded to the downregulation of catalase and the upregulation of ACOX1 activity. However, in Acox1−/− cells, which represent a model of oxidative stress, an increase in catalase activity was observed only after 48 hours of treatment, indicating an alternative mode of action of the extract. It was also observed the reduced ROS and NO formation in cells, indicating the pronounced antioxidant capacity of R. nigrum extract in the investigated cell-models.
A sensing paradigm of analog-to-feature (A2F) based on the MEMS accelerometer-integrated reservoir computing
Authors: Zou Xudong , Yang Wuhao , Sun Jie , Guo Xiaowei , Xiong Xingyin , Wang Zheng , Li Zhitian , Bie Xiaorui 
Published: June 2024

Abstract

The current boom in Micro-electromechanical Systems combined with the advent of the physical reservoir computing make it possible to break through the bottleneck of traditional sensing paradigms based on physically separated sensors and processors. Here, we report a pioneering work on a novel sensing paradigm of analog-to-feature using a stiffness modulated micromechanical resonant accelerometer. Specifically, the amplitude-acceleration nonlinearity of the accelerometer and its transient nonlinearity serve as the nonlinear dynamics of our physical reservoir computing to categorize the accelerometer input. Furthermore, we perform ten times tenfold cross-validation tests and use the confusion matrix to test its classification performance on a data set of the different acceleration corresponding to the different motion postures generated by a six-axis IMU. The results show a classification accuracy of 97.33%, which proves that our MEMS accelerometer-integrated reservoir computing enables data processing ability locally at the sensing side for efficient distributed processing.
Perceived stress and mobile phone addiction among China Nursing Students: The chain-mediating role of anxiety and flow experience
Authors: Rong Shuiqing , Yang Zhaonan , Yang Lida , Li Yuanbo , Zhou Jiawei , Hu Xiaomeng , Ke Siyuan , Wang Qiongyi , Fan Qinghe , Duan Wei , Wang Yizhi , Liu Junling , Yang Yanjie , Yang Xiuxian 
Published: June 2024

Abstract

Background: The relationship between perceived stress and mobile phone addiction among nursing students requires elucidation, alongside the clarification of potential explanatory pathways for associated risks.
Methods: 4179 freshmen and sophomore students participated in this investigation. Several assessment tools were employed, including the Chinese Perceived Stress Scale, Generalized Anxiety Disorder-7 scale, Flow State Scale, and Mobile Phone Addiction Tendency Scale. Data analysis encompassed descriptive statistics, independent sample T-tests, Pearson correlation analysis, and Hayes' PROCESS 3.5 for chain-mediated modeling.
Results: Pearson correlation analysis unveiled positive associations between perceived stress and anxiety (r=0.507, p<0.01), flow experience (r=0.657, p<0.01), and mobile phone addiction (r=0.578, p<0.01). Anxiety exhibited positive correlations with flow experience (r=0.490, p<0.01) and mobile phone addiction (r=0.564, p<0.01). Additionally, flow experience showed a positive relationship with mobile phone addiction (r=0.579, p<0.01). Chain mediation analysis revealed that anxiety [mediated effect: 26.62%, SE=0.014, 95% CI= (0.182,0.237)] and flow experience [mediated effect: 25.05%, SE=0.014, 95% CI= (0.169,0.225)] partially mediated the link between perceived stress and mobile phone addiction, respectively. Perceived stress exerted its influence on mobile phone addiction through the chain-mediated effects of anxiety and flow experience [mediating effect: 4.98%, SE=0.039, 95% CI= (0.031, 0.047)].
Conclusion: Perceived stress not only directly impacts the mobile phone addiction tendencies of nursing students but also exerts indirect effects via the independent mediation of anxiety and flow experience. Moreover, perceived stress affects mobile phone addiction through the chain-mediated pathway involving both anxiety and flow experience.
Probabilistic Approach to Assessing the Radiation Shielding Capabilities of Carbonated Hydroxyapatite Modified by Carbonate-to-Phosphate Ratio and Temperature
Authors: Khattari Z. Y. , Mahdy Ebrahim A. 
Published: June 2024

Abstract

This study utilizes the calculated mass attenuation coefficient (MAC) in conjunction with probability theory to evaluate the suitability of various energy regions for medical applications by analyzing the energy of transmitted radiation beams through samples. The findings reveal a strong correlation between the shielding properties of carbonated hydroxyapatite (CHA) compounds, the unit cell crystalline parameters, and preparation temperature. Notably, an introduction of a probabilistic methodology for transmission energy assessment, identifying the photoelectric process as the most probable scattering process with an average energy of equal 8.7 MeV. Specifically, the outcomes show that CHA compounds with a 4 mol% molar ratio exhibit the most promising characteristics for radiation shielding. An establishment of a connection between their shielding properties and atomic molar volume, preparation temperature, and structural configurations within bioactive networks, providing valuable insights for applications compared to standard glasses like RS-520.
Extracellular Vesicles Containing GAS6 Protect the Liver from Ischemia-Reperfusion Injury by Enhancing Macrophage Efferocytosis via MerTK-ERK-COX2 Signaling
Authors: Di Guohu , Miao Longyu , Yu Chaoqun , Guan Ge , Luan Xiaoyu , Jin Xiaoshuang , Pan Meiqi , Yang Yuzhen , Yan Jiaoyang , Chen Peng 
Published: June 2024

Abstract

Hepatic ischaemia-reperfusion injury (HIRI) is a significant issue during liver transplantation and surgery, contributing to the liver failure or even mortality. Although extracellular vesicles derived from mesenchymal stem cells (MSC-EVs) have shown substantial potentials in cell replacement therapy of various organ IRIs, the precise mechanisms remain unclear. In this study, we demonstrate that systemic MSC-EVs administration is predominantly absorbed by macrophages, and verified that it could significantly reduce the liver injury and inflammatory response in mice suffered from HIRI. Furthermore, treatment with MSC-EVs induces macrophage polarization toward an anti-inflammatory phenotype. Mechanistically, proteomic profiling reveals an enrichment of growth arrest-specific 6 (GAS6) in MSC-EVs, significantly promoting the activation of myeloid-epithelial-reproductive tyrosine kinase/extracellular regulated protein kinases/cyclooxygenase 2 (MerTK/ERK/COX2) signaling pathway in macrophages and further enhancing their efferocytosis efficiency. Knockdown of GAS6 via lentiviral transfection or inhibition of MerTK using UNC2025 partially eliminates the protective effects of MSC-EVs on macrophage efferocytosis and liver injury. Overall, our findings support that MSC-EVs enriched GAS6 execute an anti-inflammation effect, highlighting that treatment bases on the modulation of macrophage function by MSC-EVs as a promising approach in IRI.
The Relationship between CXCR6+CD8+T cells and Clinicopathological Parameters in Patients with primary biliary cholangitis
Authors: Shi Huilian , Xu Xiangtao , Wang Shuangshuang , Chen Qinlei , Zhang Fan , Guo Haiyan , Lu Weiting , Qiao Fei 
Published: June 2024

Abstract

Background
CXCR6 + CD8 + T cells have been implicated in the pathogenesis of various liver and autoimmune diseases. However, their involvement in Primary Biliary Cholangitis (PBC) has not been eluciated.
Methods
We used immunohistochemistry and flow cytometry to quantify CXCR6 + CD8 + T cells in hepatic tissue and peripheral blood samples obtained from CXCR6 + CD8 + T cells obtained from PBC patients. Then, we performed comprehensive statistical analyses to access the correlation between the abundance of these cells and clinical as well as pathological data across different stages of PBC.
Results
Our research revealed that CXCR6 + cell frequencies in CD3 + CD8 + T cells from PBC patients significantly exceeded that of healthy controls (HCs) (2.24% vs. 0.61%, p < 0.01). A similar pattern emerged for hepatic CXCR6 + CD8 + T cell counts, which were notably higher in the PBC cohort compared to HCs. Our cohort consisted of 118 PBC patients, categorized into 62 early-stage (E-PBC) and 56 late-stage (L-PBC) cases. Notably, significant disparities existed between these groups in terms of liver enzyme and lipid profile levels (p < 0.05), with no notable differences observed in gender, age, blood counts, cholesterol levels, or autoantibodies (p > 0.05). Intriguingly, the quantity of hepatic CXCR6 + CD8 + T cells per high power field (HPF) was significantly elevated in both E-PBC and L-PBC patients as opposed to normal liver samples, indicating a substantial increase in these cells across all stages of PBC (p = 0.000). Spearman’s rank correlation analysis using showed a positive correlation between CXCR6 + CD8 + T cell counts and serum levels of Alkaline Phosphatase (AKP) and Gamma-Glutamyl Transferase (GGT), while revealing a negligible correlation with Alanine Aminotransferase (ALT) and Aspartate Aminotransferase (AST). Subsequent findings indicated significant variances in CXCR6 + cell numbers not only among different PBC stages but also across various degrees of inflammation and fibrosis (p ≤ 0.007). In a follow-up study post-Ursodeoxycholic Acid (UDCA) treatment, stark differences were identified in biochemical and immunohistochemical profiles between responder (31 patients) and non-responder (33 patients) groups (p < 0.05). A Wilcoxon rank-sum test further demonstrated a significant difference in the level of hepatic CXCR6 + CD8 + T cells between these two response groups (p = 0.002).
Conclusion
CXCR6 + CD8 + T cells play a vital role in the pathogenesis of PBC, exhibiting correlations with the extent of inflammation, staging of liver fibrosis, and response to pharmacological interventions in PBC patients.
Electronic Upcycling of Corn Straw: Biobased Elastic Conductive Cross-linked Network Eco-friendly Hydrogels with multifunction for Flexible Wearable Sensors
Authors: Wang Junyao , Liu Huan , Wang Taipeng , Gao Guangze , Li Yaqun , Ren Dingyi , Quan Jingran , Xun Jianxin , Pan Hongxu , Huang Yuhan , Huang Yuyang 
Published: June 2024

Abstract

The conductive hydrogels based on biomaterials have garnered much attention. However, the utilization of corn straw, an agricultural waste, as a gel substrate can achieve the recycling of agro-waste and the development of advanced electronic materials, which has a dual benefit strategy seems to be overlooked. In this study, a corn straw pith (CSP) based multifunctional conductive hydrogel (CSP-MCG) was prepared by introducing CSP, which presents oxygen-rich groups, into the three-dimensional crosslinking network system of the hydrogel. The CSP-MCG has mechanical elasticity (maximum tensile rate of 340%) and responsiveness to external environmental factors (mechanical strain, temperature, humidity): Under different tensile strain conditions, the fatigue response is stable and the sensitivity is high (GF=2.8, R2=0.996, Tres=0.075s);In temperature range 20℃-50℃, it exhibits great performance (R2=0.993, TCR=1.34%/℃, Tres=0.5s); Within the humidity range RH29%-79%, the signal changes steadily and the response time is short (Tres=0.2s). In addition, CSP-MCG not only exhibits responsiveness to various external stimuli, but also demonstrates the ability to differentiate between these stimuli based on variation trend in its resistance and capacitance. Due to its exceptional multi-functional sensing capabilities and environmental attribute (recyclability and degradability), CSP-MCG can be as green flexible electronic material in practical applications such as electronic skin, human-computer interaction and flexible wearable sensors.
Determinants of multiple maize technology packages adoption in Ethiopia: Evidence from Sidama region
Authors: Guye Ashenafi , Tefera Tewodros , Sileshi Million , Edriss Abdi-Khalil 
Published: June 2024

Abstract

Adoption of improved agricultural technology packages is vital in Ethiopia, as the expansion of cultivable land appears nearly exhausted and population size has skyrocketed. However, the country has been shown low adoption rate. Thus, this study aimed to explore the factors that hinder or facilitate the adoption of multiple maize technology packages in the northern Sidama zone of Ethiopia. A multistage sampling procedure was applied to gather cross-sectional data from 424 farm households owning 545 maize plots. A multivariate probit model was applied to address the study objectives. Of total plots, improved maize seed, fertilizer, and row planting were adopted on about 54, 45, and 44 percent, respectively. The conditional probability results have also confirmed that maize technology packages have complementarity (positive relationship). This infers that agriculture-focused policies that influence the adoption of a single component of technology packages can have a reinforcing advantage over the adoption of other technologies. Furthermore, the results from model showed that farmers with higher family size, plot size, age, tropical livestock unit, ox (en), off-farm income, access to credit and extension services, membership in institutions, and the number of plots are more likely to adopt at least one of the improved maize technology packages. However, distance to maize plots affected adoption negatively. Therefore, it is crucial to reinforce and deliver quality extension services, provide credit access, motivate youth to be involved in farming activities, inspire membership and ease the system to access inputs and technologies for broader adoption of technology packages.