bluuebunny/crossref_metadata_2025_split huggingface dataset:
CXCR4‐independent rescue of the myeloproliferative defect of the gata1low myelofibrosis mouse model by Aplidin®
Authors: Verrucci Maria , Pancrazzi Alessandro , Aracil Miguel , Martelli Fabrizio , Guglielmelli Paola , Zingariello Maria , Ghinassi Barbara , D'Amore Emanuela , Jimeno José , Vannucchi Alessandro M. , Migliaccio Anna Rita 
Published: May 2010

AbstractThe discovery of JAK2 mutations in Philadelphia‐negative myeloproliferative neoplasms has prompted investigators to evaluate mutation‐targeted treatments to restore hematopoietic cell functions in these diseases. However, the results of the first clinical trials with JAK2 inhibitors are not as promising as expected, prompting a search for additional drugable targets to treat these disorders. In this paper, we used the hypomorphic Gata1low mouse model of primary myelofibrosis (PMF), the most severe of these neoplasms, to test the hypothesis that defective marrow hemopoiesis and development of extramedullary hematopoiesis in myelofibrosis is due to insufficient p27Kip1 activity and is treatable by Aplidin®, a cyclic depsipeptide that activates p27Kip1 in several cancer cells. Aplidin® restored expression of Gata1 and p27Kip1 in Gata1low hematopoietic cells, proliferation of marrow progenitor cells in vitro and maturation of megakaryocytes in vivo (reducing TGF‐β/VEGF levels released in the microenvironment by immature Gata1low megakaryocytes). Microvessel density, fibrosis, bone growth, and marrow cellularity were normal in Aplidin®‐treated mice and extramedullary hematopoiesis did not develop in liver although CXCR4 expression in Gata1low progenitor cells remained low. These results indicate that Aplidin® effectively alters the natural history of myelofibrosis in Gata1low mice and suggest this drug as candidate for clinical evaluation in PMF. J. Cell. Physiol. 225: 490–499, 2010. © 2010 Wiley‐Liss, Inc.
Evidences of a role for eukaryotic translation initiation factor 5A (eIF5A) in mouse embryogenesis and cell differentiation
Authors: Parreiras‐e‐Silva Lucas T. , Luchessi Augusto D. , Reis Rosana I. , Oliver Constance , Jamur Maria C. , Ramos Ricardo G.P. , Oliveira Eduardo B. , Curi Rui , Costa‐Neto Claudio M. 
Published: May 2010

AbstractEukaryotic translation initiation factor 5A (eIF5A) has a unique character: the presence of an unusual amino acid, hypusine, which is formed by post‐translational modifications. Even before the identification of hypusination in eIF5A, the correlation between hypusine formation and protein synthesis, shifting cell proliferation rates, had already been observed. Embryogenesis is a complex process in which cellular proliferation and differentiation are intense. In spite of the fact that many studies have described possible functions for eIF5A, its precise role is under investigation, and to date nothing has been reported about its participation in embryonic development. In this study we show that eIF5A is expressed at all mouse embryonic post‐implantation stages with increase in eIF5A mRNA and protein expression levels between embryonic days E10.5 and E13.5. Immunohistochemistry revealed the ubiquitous presence of eIF5A in embryonic tissues and organs at E13.5 day. Interestingly, stronger immunoreactivity to eIF5A was observed in the stomodeum, liver, ectoderm, heart, and eye, and the central nervous system; regions which are known to undergo active differentiation at this stage, suggesting a role of eIF5A in differentiation events. Expression analyses of MyoD, a myogenic transcription factor, revealed a significantly higher expression from day E12.5 on, both at the mRNA and the protein levels suggesting a possible correlation to eIF5A. Accordingly, we next evidenced that inhibiting eIF5A hypusination in mouse myoblast C2C12 cells impairs their differentiation into myotubes and decreases MyoD transcript levels. Those results point to a new functional role for eIF5A, relating it to embryogenesis, development, and cell differentiation. J. Cell. Physiol. 225: 500–505, 2010. © 2010 Wiley‐Liss, Inc.
Establishment of a new conditionally immortalized human brain microvascular endothelial cell line retaining an in vivo blood–brain barrier function
Authors: Sano Yasuteru , Shimizu Fumitaka , Abe Masaaki , Maeda Toshihiko , Kashiwamura Yoko , Ohtsuki Sumio , Terasaki Tetsuya , Obinata Masuo , Kajiwara Koji , Fujii Masami , Suzuki Michiyasu , Kanda Takashi 
Published: May 2010

AbstractThe breakdown of the blood–brain barrier (BBB) has been considered to be a key step in the disease process of a number of neurological disorders such as cerebral ischemia and Alzheimer's disease. Many in vitro BBB models derived from animal tissues have been established to elucidate the mechanism of BBB insufficiency. However, only a few human immortalized in vitro BBB models have been reported. In the present study, a temperature‐sensitive SV40‐T antigen was introduced to immortalize cells using a retrovirus to obtain a better human in vitro BBB model which sustains physiological properties. This endothelial cell (EC) line, termed TY08, showed a spindle‐shaped morphology. The cells expressed all key tight junctional proteins, such as occludin, claudin‐5, zonula occludens (ZO)‐1 and ZO‐2 at their cell‐to‐cell boundaries, and had low permeability to inulin across its monolayer. The cells also expressed various influx and efflux transporters and exhibited the functional expression of p‐glycoprotein. Furthermore, the TY08 cells grew and proliferated well under the permissive temperature and stopped growing under the non‐permissive temperature to serve as physiological ECs forming the BBB. Thus, conditionally immortalized TY08 cells retaining the in vivo BBB functions should facilitate analyses for determining the pathophysiology of various neurological diseases. J. Cell. Physiol. 225: 519–528, 2010. © 2010 Wiley‐Liss, Inc.
Synthesis of 4,7‐disubstituted spirobenzofuran‐2(3H)‐cyclohexanes
Authors: Bradbury Barton J. , Sindelar Robert D. 
Published: May 2010

AbstractA general synthetic strategy was designed for the preparation of 7‐substituted‐4‐methoxy‐ and 4‐hydroxyspiro[benzofuran‐2(3H)‐cyclohexanes] 5 and 6 (Figure 1) using successive, regioselective hetero‐atom‐facilitated aromatic lithiation reactions and subsequent reaction with various electrophiles.
Synthesis of certain 1‐β‐D‐ribofuranosyl‐1,2‐dihydro‐2‐oxopyridines structurally related to nicotinamide ribonucleoside
Authors: Hanna Naeem B. , Joshi Ramachandra V. , Larson Steven B. , Robins Roland K. , Revankar Ganapathi R. 
Published: May 2010

AbstractSeveral substituted 1‐β‐D‐ribofuranosyl‐1,2‐dihydro‐2‐oxopyridines have been prepared as congeners of nicotinamide ribonucleoside. Direct glycosylation of the silylated 3‐ethylcarboxylate 5 or 3‐carbamoyl 6 derivative of 1,2‐dihydro‐2‐oxopyridine with 1,2,3,5‐tetra‐O‐acetyl‐β‐D‐ribofuranose (7) in the presence of trimethylsilyl triflate gave the corresponding blocked nucleosides 8 and 9, respectively in good yield. Ammonolysis of 8 and 9 with methanolic ammonia furnished 1‐β‐D‐ribofuranosyl‐1,2‐dihydro‐2‐oxopyridine‐3‐carboxa‐mide (10), the structure of which was established by single‐crystal X‐ray diffraction analysis. Thiation of 9 with Lawesson's reagent and subsequent deacetylation of the thiated product 11 with methanolic ammonia furnished 1‐β‐D‐ribofuranosyl‐1,2‐dihydro‐2‐oxopyridine‐3‐thiocarboxamide (12). Modification of the carbo‐nitrile function of 1‐(2,3,5‐tri‐O‐acetyl‐β‐D‐ribofuranosyl)‐1,2‐dihydro‐2‐oxopyridine‐4‐carbonitrile (13) gave a series of 4‐substituted‐1‐β‐D‐ribofuranosyl‐1,2‐dihydro‐2‐oxopyridines, in which the 4‐substituent is a thiocarboxamide 15, carboxamide 16, carboxamidoxime 17, carboxamidine 18 and aminomethyl 19 group. None of these compounds exhibited any significant antitumor or antiviral effects in vitro.
Preparation of Metal‐free phthalocyanine using the benzenetellurolate ion
Authors: Kitahara Kiyoshi , Asano Toyofumi , Tokita Sumio , Nishi Hisao 
Published: May 2010

AbstractMetal‐free phthalocyanine was prepared from o‐phthalonitrile using the benzenetellurolate ion in ethanol. Furthermore, the yield of metal‐free phthalocyanine using the benzenetellurolate ion was compared with that using the benzeneselenolate ion or the benzenethiolate ion.
Inline pneumatically assisted atmospheric pressure matrix‐assisted laser desorption/ionization ion trap mass spectrometry
Authors: Navare Arti T. , Fernández Facundo M. 
Published: May 2010

AbstractAtmospheric pressure (AP) matrix‐assisted laser desorption/ionization (MALDI) is known to suffer from poor ion transfer efficiencies as compared to conventional vacuum MALDI (vMALDI). To mitigate these issues, a new AP‐MALDI ion source utilizing a coaxial gas flow was developed. Nitrogen, helium, and sulfur hexafluoride were tested for their abilities as ion carriers for a standard peptide and small drug molecules. Nitrogen showed the best ion transport efficiency, with sensitivity gains of up to 1900% and 20% for a peptide standard when the target plate voltage was either continuous or pulsed, respectively. The addition of carrier gas not only entrained the ions efficiently but also deflected background species and declustered analyte–matrix adducts, resulting in higher absolute analyte signal intensities and greater signal‐to‐noise (S/N) ratios. With the increased sensitivity of pneumatically assisted (PA) AP‐MALDI, the limits of detection of angiotensin I were 20 or 3 fmols for continuous or pulsed target plate voltage, respectively. For analyzing low‐mass analytes, it was found that very low gas flow rates (0.3–0.6 l min−1) were preferable owing to increased fragmentation at higher gas flows. The analyte lability, type of gas, and nature of the extraction field between the target plate and mass spectrometer inlet were observed to be the most important factors affecting the performance of the in‐line PA‐AP‐MALDI ion source. Copyright © 2010 John Wiley & Sons, Ltd.
Anomeric discrimination and rapid analysis of underivatized lactose, maltose, and sucrose in vegetable matrices by U‐HPLC–ESI‐MS/MS using porous graphitic carbon
Authors: Gabbanini Simone , Lucchi Elena , Guidugli Federico , Matera Riccardo , Valgimigli Luca 
Published: May 2010

AbstractLactose intolerance is a common condition caused by intestinal lactase deficiency, and a lactose‐free diet represents the simplest way to avoid gastrointestinal symptoms. The emerging use of dietary supplements requires analytical tools that are capable of assessing these analytes, particularly for those based on dry herbal extracts that contain lactose together with maltose and sucrose, because of cross‐contamination and/or deliberate addition as excipient. Electrospray ionization mass spectrometry (ESI‐MS) and MS/MS are valuable detection methods for underivatized disaccharides; however, the absence of distinctive ions and collision‐induced dissociation (CID) fragmentation patterns does not allow discrimination of stereoisomers without good chromatographic resolution. We developed an ultrahigh performance liquid chromatography‐ESI (U‐HPLC‐ESI) approach, based on porous graphitic carbon (PGC) columns, working at 5 °C to separate and detect the disaccharides in their anomeric forms as formate adducts obtained directly in‐column by eluting with formate buffer/acetonitrile gradient mixtures. Using a Paul trap, we monitored the adducts [M + HCOO]− at m/z 387 in ESI negative mode (MS1) as well as the CID fragment ion [M − H]− at m/z 341 (MS2) and used MS3 fragment ions at m/z 178 and 161 to confirm disaccharides identity in complex vegetable matrices. Complete resolution of lactose α‐ and β‐anomers, maltose α‐ and β‐anomers, and sucrose was obtained with R ≥ 2.0 for all peaks and selectivity α = 1.2 between α‐ and β‐anomers of lactose. The limits of detection were in the range of 3–7 µg/l (ppb) for the target disaccharides. Because of the rapidity and good anomeric discrimination, the described method represents an alternative tool to investigate the mutarotation phenomenon for reducing disaccharides. Copyright © 2010 John Wiley & Sons, Ltd.
Simultaneous determination of the composition and temperature gradients in the vicinity of boiling bubbles in liquid binary mixtures using one‐dimensional Raman measurements
Authors: Knauer O. S. , Lang M. C. , Braeuer A. , Leipertz A. 
Published: May 2010

AbstractThe ability to transport large quantities of heat by nucleate boiling is reduced for mixtures compared to their pure components. To study the heat and mass transfer during the boiling process, a one‐dimensional Raman spectroscopy setup was applied to measure, for the first time to the best of our knowledge, the composition and temperature gradients in the vicinity of single boiling bubbles of a mixture of acetone and isopropanol. A novel evaluation strategy allowed the simultaneous detection of the mixture composition and temperature from the CH and the OH Raman spectra. The composition gradients arise because of rectification effects, which cause unlike compositions in the gas and liquid phases. Heat transfer from the boiling bubble to the surrounding liquid induces temperature gradients. High temporal and spatial resolution measurements using an imaging spectrograph and an electron multiplying charge‐coupled device camera revealed large composition and temperature gradients with similar shapes. The accuracy of the composition results was validated by a species conservation calculation. Copyright © 2010 John Wiley & Sons, Ltd.
NMR‐based analysis of structure of heteroleptic triple‐decker (phthalocyaninato) (porphyrinato) lanthanides in solutions
Authors: Birin Kirill P. , Gorbunova Yulia G. , Tsivadze Aslan Yu. 
Published: May 2010

AbstractA novel approach for the structural analysis of heteroleptic triple‐decker (porphyrinato)(phthalocyaninato) lanthanides(III) in solutions is developed. The developed approach consists in molecular mechanics (MM+) optimization of the geometry of the complex taking into account the lanthanide‐induced shift (LIS) datasets. LISs of the resonance peaks in 1H NMR spectra of a series of symmetric complexes [An4P]Ln[(15C5)4Pc]Ln[An4P], where An4P2− is 5,10,15,20‐tetrakis(4‐methoxyphenyl)porphyrinato‐dianion, [(15C5)4Pc]2− is 2,3,9,10,16,17,24,25‐tetrakis(15‐crown‐5)phthalocyaninato‐dianion and Ln = La, Ce, Pr, Nd, Sm, Eu, are analyzed. Analysis of LISs showed two sets of protons in the molecule with opposite signs of shift. Two‐nuclei analysis of LISs testifies isostructurality of the whole series of investigated complexes in solution despite contraction of the lanthanide ions. Model‐free separation of contact and dipolar contributions of LISs was performed with one‐nucleus technique and did not show changes in contact and dipolar terms within the investigated series. MM+ optimization of the molecular structure allowed the interpretation of features of LIS for each particular group of protons. Parameterization of MM + ‐optimized model of molecule with values of structure‐dependent dipolar contributions of LIS allows the development of the precise structural model of the triple‐decker complex in solution. This approach allows the determination of the geometry and structure of the sandwich macrocyclic tetrapyrrolic complexes together with conformational analysis of flexible peripheral substituents in solutions. The developed method can be applied with minor modifications for the determination of structural parameters of other types of lanthanides(III) complexes with tetrapyrrolic ligands and also supramolecular systems based on them. Copyright © 2010 John Wiley & Sons, Ltd.
Glutaraldehyde crosslinked gelatin/hydroxyapatite nanocomposite scaffold, engineered via compound techniques
Authors: Azami Mahmoud , Rabiee Mohammad , Moztarzadeh Fathollah 
Published: May 2010

AbstractIn this study, a scaffold was designed to be used in bone tissue repair and the effect of glutaraldehyde (GA) concentration as crosslinking agent was investigated. To mimic the mineral and organic component of natural bone, hydroxyapatite (HAp) and gelatin (GEL) were used as the main components of this composite. Nanopowders of HAp were synthesized and also used together with GEL to engineer a three‐dimensional nanocomposite scaffold. The results show that GEL/HAp nanocomposite is porous with three‐dimensional interconnected structure, pore sizes ranging from 300 to 500 μm, and about 85% porosity. In addition, increasing GA concentration provokes the enhancement of compressive strength until 1 w/v% GA solution followed by a reduction to 2.5%, whereas it causes work fracture to decrease. It was concluded that optimum concentration for crosslinking GEL matrix for this purpose is 1 w/v% GA solution. A specific combination of commonly used techniques applied to engineer a scaffold with almost ideal properties intended for the bone tissue engineering is introduced. In addition, scaffolds that are prepared via this compound process has the potential to be used in the solid free form applications and so being formed in any dimension and geometry relevant to the defect size and shape. POLYM. COMPOS., 31:2112–2120, 2010. © 2010 Society of Plastics Engineers
Motor performance is better than normal in preschool children with cystic fibrosis
Authors: Gruber Wolfgang , Orenstein David M. , Paul Karl , Hüls Gerd , Braumann Klaus M. 
Published: May 2010

AbstractThe aim of the present study was to assess the motor performance in preschool children with a reliable and valid test battery developed to identify motor dysfunction and normal motor development in children aged from 4 to 6 years. Several aspects of motor performance were examined in 29 preschool children with cystic fibrosis (CF) age range 4–6 years (mean 5.2 ± 0.8 years), FEV1 97.2 ± 15.3pred and compared to with 22 healthy children of the same age 5.5 ± 0.8 years. All children performed the “Motoriktest fuer 4‐6jaehrige Kinder” (MOT) assessing seven different aspects of motor performance. Compared to healthy children, test score “Motor Quotient” (MQ) as the mean of all test items was significantly higher (P < 0.05) in children with CF (108.1 ± 16 vs. 93.5 ± 17.9). In both groups, the MQ can be classified as normal. Children with CF scored higher in MOT subtests “Agility and Coordination” (P < 0.05) and “Balance” (P < 0.01) than healthy children but not in the other subtests. We speculate that chest physiotherapy in preschool children with CF may have an effect on motor performance in general and in some aspects of motor performance. Pediatr Pulmonol. 2010; 45:527–535. © 2010 Wiley‐Liss, Inc.
Probiotic supplementation affects pulmonary exacerbations in patients with cystic fibrosis: A pilot study
Authors: Weiss Batia , Bujanover Yoram , Yahav Yaakov , Vilozni Daphna , Fireman Elizabeth , Efrati Ori 
Published: May 2010

AbstractObjectiveProbiotics reduce intestinal inflammation in, and Lactobacillus GG (LGG) reduces pulmonary exacerbation rate cystic fibrosis (CF) patients. We intended to determine the effect of a mixed probiotic preparation on pulmonary exacerbations and inflammatory characteristics of the sputum in CF patients.Study DesignA prospective pilot study of 10 CF patients with mild–moderate lung disease and Pseudomonas aeruginosa colonization, treated with probiotics for 6 months. Pulmonary function tests (PFT's), sputum cultures with semi‐quantitative bacterial analysis, and sputum neutrophil count and interleukin‐8 (IL‐8) levels were compared to pre‐treatment and post‐treatment values. The rate of pulmonary exacerbations was compared to 2 years prior to the study.ResultsThe exacerbation rate was significantly reduced in comparison to the previous 2 years and to 6 months post‐treatment (P = 0.002). PFT's have not changed at the end of treatment and during 6 months post‐treatment. No change in sputum bacteria, neutrophil count, and IL‐8 levels was observed.ConclusionProbiotics reduce pulmonary exacerbations rate in patients with CF. Probiotics may have a preventive potential for pulmonary deterioration in CF patients. Pediatr Pulmonol. 2010; 45:536–540. © 2010 Wiley‐Liss, Inc.
Endoscopy for the initial suspicion of vascular rings in tracheoesophageal compressions: Correlation with surgical findings
Authors: Furuya María E.Y. , Vargas Mario H. , Ramírez‐Figueroa Jorge Luis , Félix‐Heredia José Luis , González‐Ortíz Beatriz , Rodríguez‐Hernández Lydia , Vera‐Canelo José Manuel 
Published: May 2010

AbstractVascular rings (VR) may produce extrinsic compression of trachea and/or esophagus. Diagnosis relies on nonspecific clinical data from resulting compression and image studies, mainly angiography, computed tomography, and cardiovascular MRI. Because of their low incidence, diagnosis is often missed. The role of bronchoscopy and esophagoscopy is controversial, but these procedures might establish for the first time the diagnostic suspicion of VR. This communication was aimed to determine to what extent endoscopic manifestations lead the clinician to suspect the presence of a VR and, moreover, to define their correlation with surgical findings.MethodsChildren with endoscopic diagnosis of VR submitted to surgery were retrospectively analyzed.ResultsTwenty patients fulfilled inclusion criteria. They were submitted to 19 bronchoscopies and 16 esophagoscopies, and in 60% cases they raised the first suspicion of VR. Diagnostic agreement of the type of VR between endoscopy and surgery was 85%. Bronchoscopy diagnosed 12 VR, and in 10 cases predicted the specific type of VR. Additional relevant findings were detected in nine patients (47.4%). Esophagoscopy diagnosed VR in 14 patients, predicted the specific type in 78.6% and detected all aberrant right subclavian artery cases.ConclusionsInitial suspicion of VR was raised by bronchoscopic and/or esophagoscopic findings in 60% cases. Endoscopy proved to be a valuable tool to anticipate the type of malformation and to disclose associated abnormalities. Pediatr Pulmonol. 2010; 45:560–565. © 2010 Wiley‐Liss, Inc.
Reactive Flow Model Parameter Estimation Using Genetic Algorithms
Authors: Baranda Ribeiro Jose , Mendes Ricardo , Silva Cristóvão 
Published: May 2010

AbstractAn original real‐coded genetic algorithm methodology that has been developed for the estimation of the parameters of the Tarver reactive flow model of shock initiation and detonation of heterogeneous solid explosives is described in detail. This methodology allows, in a single optimisation procedure and without the need for a starting solution, to search for the 15 parameters of the reaction rate law of the reactive flow model that fit the numerical results to the experimental ones. The developed methodology was applied and tested with an experimental situation, described in detail in the literature, involving the acceleration of a tantalum metal plate by an LX‐17 explosive charge. The obtained parameters allow a very good description of the experimental results and are close to the ones originally used by Tarver and co‐authors in their simulation of the phenomenon.
Characterization of a Series of Nitrogen‐Rich Molecules using Laser Induced Breakdown Spectroscopy
Authors: De Lucia Frank C. , Gottfried Jennifer L. 
Published: May 2010

AbstractA series of nitrogen‐rich molecules, such as 5‐aminotetrazolium nitrate (HAT‐NO3) and hydrazinebistetrazole (HBT), bis(2,2,2‐trinitroethyl)‐hydrazodicarboxylate (BTHC), cyclotrimethylene trinitramine (RDX), trinitrotoluene (TNT), melamine, sucrose, and L‐glutamine were studied using laser‐induced breakdown spectroscopy (LIBS). The atomic emission intensities and intensity ratios of the constituent elements from the LIBS spectrum were shown to correlate with the mole fractions and stoichiometries of the molecules. In addition, the amount of oxygen present in the molecule influenced the emission intensities of molecular fragments such as C2. Finally, we used principal components analysis to analyze the data from the LIBS spectra and separate the different organic molecules based on the atomic emission intensities and ratios.