bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anatomical determinants of occipitocervical fusion in skull base chordoma resection: a systematic review of the literature with illustrative cases
Authors: Golub Danielle , Küffer Alexander F. , Garrel Shimon , Zandpazandi Sara , McBriar Joshua D. , Modi Siddhi , Papadimitriou Kyriakos , Costantino Peter D. , Sciubba Daniel M. , Dehdashti Amir R. 
Published: May 2024

OBJECTIVE
Skull base chordomas are rare, locally osseo-destructive lesions that present unique surgical challenges due to their involvement of critical neurovascular and bony structures at the craniovertebral junction (CVJ). Radical cytoreductive surgery improves survival but also carries significant morbidity, including the potential for occipitocervical (OC) destabilization requiring instrumented fusion. The published experience on OC fusion after CVJ chordoma resection is limited, and the anatomical predictors of OC instability in this context remain unclear.

METHODS
PubMed and Embase were systematically searched according to the PRISMA guidelines for studies describing skull base chordoma resection and OC fusion. The search strategy was predefined in the authors’ PROSPERO protocol (CRD42024496158).

RESULTS
The systematic review identified 11 surgical case series describing 209 skull base chordoma patients and 116 (55.5%) who underwent OC instrumented fusion. Most patients underwent lateral approaches (n = 82) for chordoma resection, followed by midline (n = 48) and combined (n = 6) approaches. OC fusion was most often performed as a second-stage procedure (n = 53), followed by single-stage resection and fusion (n = 38). The degree of occipital condyle resection associated with OC fusion was described in 9 studies: total unilateral condylectomy reliably predicted OC fusion regardless of surgical approach. After lateral transcranial approaches, 4 studies cited at least 50%–70% unilateral condylectomy as necessitating OC fusion. After midline approaches—most frequently the endoscopic endonasal approach (EEA)—at least 75% unilateral condylectomy (or 50% bilateral condylectomy) led to OC fusion. Additionally, resection of the medial atlantoaxial joint elements (the C1 anterior arch and tip of the dens), usually via EEA, reliably necessitated OC fusion. Two illustrative cases are subsequently presented, further exemplifying how the extent of CVJ bony elements removed via EEA to achieve complete chordoma resection predicts the need for OC fusion.

CONCLUSIONS
Unilateral total condylectomy, 50% bilateral condylectomy, and resection of the medial atlantoaxial joint elements were the most frequently described independent predictors of OC fusion in skull base chordoma resection. Additionally, consistent with the occipital condyle harboring a significantly thicker joint capsule at its posterolateral aspect, an anterior midline approach seems to tolerate a greater degree of condylar resection (75%) than a lateral transcranial approach (50%–70%) prior to generating OC instability.
Pengaruh Kepuasan Kerja dan Komitmen Organisasi terhadap Kinerja Karyawan dengan Organizational Citizenship Behavior (OCB) sebagai Variabel Intervening Pada Tenaga Penjualan
Authors: Aminudin Achmad , Budiarti Yuli , Indarto Indarto 
Published: May 2024

Penelitian ini dilatar belakangi oleh adanya suatu permasalahan belum tercapainya secara optimal kinerja karyawan yang sesuai dengan kemampuan dan standar kompetensi yang telah ditetapkan sehingga tidak bisa memenuhi target kerja yang telah ditetapkan. Penelitian ini bertujuan untuk menganalisis pengaruh kepuasan kerja dan komitmen organisasi terhadap kinerja karyawan dengan OCB sebagai variabel intervening. Penelitian ini menggunakan pendekatan kuantitatif. Populasi dalam penelitian ini adalah 58 responden. Sampel yang digunakan adalah dengan teknik purposive sampling. Jenis data yang digunakan adalah data primer. Teknik pengumpulan data dengan menggunakan kuesioner. Teknik analisis data yang digunakan dengan teknik analisis regresi linier berganda. Kesimpulan pada penelitian ini adalah kepuasan kerja berpengaruh positif dan signifikan terhadap kinerja karyawan. Komitmen organisasi berpengaruh positif dan signifikan terhadap kinerja karyawan. kepuasan kerja berpengaruh positif dan signifikan terhadap OCB. Komitmen organisasi berpengaruh positif dan signifikan terhadap OCB. OCB berpengaruh positif dan signifikan terhadap kinerja karyawan.Kata kunci: kepuasan kerja, kinerja karyawan, komitmen organisasi, OCB
Retrospective Review and Analysis on Outcome of Cranioplasty: A Series of 38 Cases
Authors: AKM Bazlul Karim  , Riad Habib  , Abu Tahir Mohammad Sahidullah Monsur  , Kawshik Ahmed  , Mir Shamsad Hossain  , Mithun Kumar Dey  , Atique Bin Hussain  , Adnan Moasir Hasnine  , Md Mamun Reza  , Dibakar Chaudhury  , MD Rezwan Radid  , Aziza Khatun  , Md Arif Hasan  , Kazi Shahabulbul Islam  , Md Ashiqul Muhit khan  , Md Ifran Ahmed  
Published: May 2024

Background: Cranioplasty (CP) is a straightforward procedure, it may result in a significant number of complications. These include infections, seizures, intracranial hematomas, and others.
Objective: This was a retrospective study on outcome of Cranioplasty.
Materials and Methods: This retrospective study included patients who underwent decompressive craniectomy due to traumatic brain injury with raised ICP, acute subdural hematoma, compound comminuted skull fracture & ICH, between January January 2019 to December 2020 at Enam Medical College & Hospital, Savar, Dhaka. Data were collected in pre-designed data collection sheet and were analyzed using computerbased programme statistical package for social science (SPSS) windows version 25.
Results: This study showed maximum patients (34.2%) were between 21-30 years age. Majority were male (89.5%) and only 5.9% were female. Overall rate of complications was 10.5%. Among them new onset seizure (n = 4), indrawing of skin through bone gap (n=4), hemorrhage (n=2), infection (n = 1), HCP (n = 1), and exposed implant (n=1).
Conclusion: We have found the outcome of cranioplasty was better with titanium mesh plate and screw than conventional fixation of bone with polyglactin, polypropylene suture and titanium miniplate and screw. With polypropylene and polyglactin there is more chance of inward displacement of bone fragment and with miniplate and screw there is still chance of indrawing of bone fragment through the bone gap.
Bang. J Neurosurgery 2023; 13(1): 20-23
Isolation, identification and quantification of flavonoids from the flowers of <i>Staphylea pinnata</i> L.
Authors: Sokolova A. Y. , Poluyanov A. M. , Bardakov A. I. , Sologova S. S. , Bobkova N. V. 
Published: May 2024

Introduction. Search for new plant species containing biologically active substances (hereinafter – BAS) is one of the leading tasks of pharmacognosy as a science. The search for flavonoid glycosides in plant raw materials is especially relevant, since they have anti-inflammatory, antioxidant, immunostimulating, as well as weak fungicidal and bacteriostatic action. Staphylea pinnata L. is an endemic plant of the Caucasus, cultivated not only in Georgia, but also in the Russian Federation in the Northern and Northwestern Caucasus. In foreign literature there are studies of antioxidant, antiproliferative and cytotoxic activity of leaf extracts of several species of S. pinnata L., as well as inhibitory activity of COX-1, COX-2 and LTB4 formation. Meanwhile, no serious Russian-language scientific studies on either the chemical composition or pharmacological action of generative organs of S. pinnata were found in the literature. This work is part of a comprehensive phytochemical study of S. pinnata. The aim of the work is to study the qualitative and quantitative composition of flavonoids in the studied object.Aim. To isolate, identify and quantify flavonoids in flowers and buds of Staphylea pinnata L.Materials and methods. Alcohol-water extracts from dried generative organs of the studied plant were used as analyzed solutions. Solutions were analyzed on a spectrophotometer SF-2000 (LLC "OKB Spectr", Russia) after sample preparation with aluminum chloride and on an HPLC Nexera-i LC-2040 (Shimadzu Corporation, Japan) equipped with a column and sample thermostat, degasser and autosampler using an individually selected elution gradient of the mobile phase (0.1 % orthophosphoric acid/acetonitrile solution). The primary data were processed using LabSolutions Single LC software (Shimadzu Corporation, Japan). Compounds from the flavonoid group were identified by retention times. Detection was carried out using a UV detector with an absorption wavelength of 365 ± 2 nm.Result and discussion. Alcohol-water extracts from flowers and buds of S. pinnata L. were obtained. Quantitative evaluation by spectrophotometry for flavonoid content was carried out. A gradient elution mode for HPLC was selected for simultaneous determination of 7 flavonoid glycosides. These chromatographic conditions allowed the identification and quantification of astragaline, cynaroside, cosmosiin, narcissin and rutin in flowers and buds of Staphylea pinnata L. Flavonoid glycosides: raponticin and kaempferol were not detected.Conclusion. Flavonoid glycosides were isolated from the generative organs of S. pinnata L., a technique for quantitative determination of flavonoid glycosides in alcohol-water extracts was developed, astragalin, cynaroside, cosmosiin, narcissin and rutin were detected and quantified.
Analysis of the clinical diagnostic capabilities of kidney imaging biomarkers based on diffusion-weighted imaging and ASL-perfusion data
Authors: Shkuratova Yu. Yu. , Morozova T. G. 
Published: May 2024

Acute kidney failure is a polyetiological disease that is often occurs in patients, which requires a rapid therapeutic response to avoid progression of the condition. Magnetic resonance imaging of the kidneys (MRI) improves the understanding and assessment of pathological processes, supplementing the mandatory diagnostic minimum information in the study of kidney pathology.Objective. To evaluate the clinical diagnostic capabilities of kidney imaging biomarkers using diffusion-weighted imaging (DWI) and ASL-perfusion data for intensive care unit patients.Materials and methods. At the first Clinical Hospital in Smolensk 54 patients were examined who were inpatient treatment and in the intensive care unit. All patients underwent ultrasound examination of the kidneys with assessment of blood flow in the main kidney vessels, MRI of the kidneys, with DWI of the kidney parenchyma and ASL-perfusion included in the study protocol. MRI was carried with assessed b-factor 800 and was drawn apparent diffusion coefficient (ADC) maps. When carried ASL perfusion, the field of view covered the area of the parenchyma of both kidneys. The reference method was glomerular filtration rate for all of the patients. The control group was represented by healthy people (n=59). Consent to participate in the study was obtained from patients or their legal representatives. All stages of the study comply with the normative and regulatory documentation of Ministry of Health ща the Russian Federation.Results. It was concluded that there is a significant difference in ADC for prerenal causes and in ASL for renal causes. In 9 (16.7 %) patients, kidney ADC was 1.5±0.3 mm2/sec, ASL – perfusion – ≤ 250 ml/100g/min, compared with the control group. In this group, negative dynamics were observed within a week, тnegative laboratory dynamics correlates with radiation-based kidney biomarkers (r=0,965).Conclusion. The criteria of DWI and ASL-perfusion allow us to suspect or confirm the cause of acute kidney failure. A high correlation has been established between radiation biomarkers and laboratory criteria of functional damages (r=0.998), direct correlation between imaging biomarkers and organic kidney disease (r=0,901). These biomarkers make it possible to predict the first stage of acute kidney failure (AUROC 0,995, DI 0,867–0,999).
Analisis Penentuan Komponen Kritis Small Excavator Menggunakan Metode FMEA dan Diagram Pareto
Authors: Nurjanah Diah Ayu , Kusminah Imah Luluk , Rachmat Aulia Nadia , Nabella Navies 
Published: May 2024

Kegiatan konstruksi meliputi tahapan pembangunan, pengoperasian, pemeliharaan, pembongkaran, dan pembangunan kembali suatu bangunan. Salah satu kegiatan konstruksi yang ada di Indonesia adalah pembangunan jalur ganda rel kereta api. Pada proyek pembangunan jalur ganda rel kereta api terdapat small excavator A yang sering mengalami kegagalan. Excavator tersebut digunakan pada pekerjaan galian, stripping lahan dan timbunan. Kegagalan yang terjadi dapat mengganggu pelaksanaan konstruksi dan dapat mengakibatkan perusahaan mengalami kerugian. Oleh sebab itu perlu adanya analisis terhadap komponen excavator agar diketahui komponen kritis. Pembuatan FBD (Functional Block Diagram) bertujuan untuk menggambarkan aliran kerja dari excavator sehingga memudahkan dalam pembuatan FMEA. Penentuan komponen kritis menggunakan metode FMEA (Failure mode and Effects Analysis) yang digunakan untuk mengidentifikasi bagaimana suatu peralatan, fasilitas, atau sistem mengalami kegagalan dan dampak yang ditimbulkannya. Metode FMEA memungkinkan penentuan peringkat risiko dengan menggunakan nilai RPN (Risk Priority Number). Hasil dari RPN dilakukan analisis menggunakan diagram pareto sehingga dapat ditentukan komponen kritis dari excavator . Komponen kritis dapat ditentukan dengan menggunakan prinsip diagram pareto yang mengikuti aturan 80-20 yang menyatakan bahwa 20% dari komponen akan mewakili 80% dari keseluruhan komponen. Penentuan RPN berdasarkan hasil wawancara bersama expert judgement. Penelitian menunjukkan bahwa pada sistem kerja exvavator terdapat 6 sub-sistem yakni sub-sistem elektrik, kendali, hidrolik, engine, lower structure, dan upper structure. Hasil dari FMEA dan diagran pareto menunjukkan bahwa terdapat 3 komponen kritis yakni komponen track shoe dengan failure mode baut shoe lepas memiliki nilai RPN 60, komponen hydraulic pump dengan failure mode seal kit regulator rusak memiliki nilai RPN 32, filter oli dengan failure mode rusak memiliki RPN 32.
Surgical repair of rectovaginal fistula by combined transanal and transvaginal endoscopy: a case report
Authors: He Tian , Zhang Wen , Mao Nian-fen , Bai Xuan , Zhao Lin , Yue Ke-lin , Yang Guo-qing , Rao Chun-mei , Wang Jing , Wan Ping , Guo Qiang , Zuo Zan 
Published: May 2024

The common causes of rectovaginal fistula include obstetric trauma, local infection, and rectal surgery, while rectovaginal fistula following hemorrhoid surgery is extremely rare. Rectovaginal fistulae (RVF) rarely heal without intervention. Surgical treatment is usually performed, but the optimal surgical method remains controversial. The patient was a 37-year-old woman who was transferred to our hospital due to an unsuccessful repair of a rectovaginal fistula after hemorrhoid surgery in a local hospital. The next day after admission, she had prophylactic ileostomy, fecal diversion and combined treatment with cephalosporin antibiotic to create a clean postoperative area. However, there was still fecal outflow from the vagina, with no significant reduction in excretion compared to previous surgery. Digestive endoscopy confirmed a failure of the repair for rectovaginal fistula. Therefore, preventive ileostomy was continued to reduce the accumulation of bacteria in the fistula and control the inflammation. After 8 weeks, the endoscopic fistula inflammation disappeared, and the condition of endoscopic surgery was considered to be mature. Subsequently, a new surgical method combining transanal endoscopy and transvaginal endoscopy was performed. After 12 weeks of surgery, a follow-up endoscopic examination showed that the fistula had been repaired and healed. During the 1-year follow-up, no serious complication was encountered, no recurrence was found, and the repair effect was satisfactory. In conclusion, a new technique combining transanal endoscopy and transvaginal endoscopy can effectively be used for the surgical repair of rectovaginal fistula after a hemorrhoid operation.
Troxerutin suppress inflammation response and oxidative stress in jellyfish dermatitis by activating Nrf2/HO-1 signaling pathway
Authors: Liu Ran , Wang Yulian , Kuai Wenhao , Li Wenting , Wang Zengfa , Xiao Liang , Wu Jianhua 
Published: May 2024

BackgroundStomolophus meleagris envenomation causes severe cutaneous symptoms known as jellyfish dermatitis. The potential molecule mechanisms and treatment efficiency of dermatitis remain elusive because of the complicated venom components. The biological activity and molecular regulation mechanism of Troxerutin (TRX) was firstly examined as a potential treatment for jellyfish dermatitis. MethodsWe examined the inhibit effects of the TRX on tentacle extract (TE) obtained from S. meleagris in vivo and in vitro using the mice paw swelling models and corresponding assays for Enzyme-Linked Immunosorbent Assay (ELISA) Analysis, cell counting kit-8 assay, flow cytometry, respectively. The mechanism of TRX on HaCaT cells probed the altered activity of relevant signaling pathways by RNA sequencing and verified by RT-qPCR, Western blot to further confirm protective effects of TRX against the inflammation and oxidative damage caused by TE. ResultsTE significantly induced the mice paw skin toxicity and accumulation of inflammatory cytokines and reactive oxygen species in vivo and vitro. Moreover, a robust increase in the phosphorylation of mitogen-activated protein kinase (MAPKs) and nuclear factor-kappa B (NF-κB) signaling pathways was observed. While, the acute cutaneous inflammation and oxidative stress induced by TE were significantly ameliorated by TRX treatment. Notablly, TRX suppressed the phosphorylation of MAPK and NF-κB by initiating the nuclear factor erythroid 2-related factor 2 signaling pathway, which result in decreasing inflammatory cytokine release. ConclusionTRX inhibits the major signaling pathway responsible for inducing inflammatory and oxidative damage of jellyfish dermatitis, offering a novel therapy in clinical applications.
Heterologous Ad26/Ad5 adenovirus-vectored vaccines elicited SARS-CoV-2-specific antibody responses with potent Fc activities
Authors: Klingler Jéromine , Kowdle Shreyas , Bandres Juan C. , Emami-Gorizi Rozita , Alvarez Raymond A. , Rao Priyanka G. , Amanat Fatima , Gleason Charles , Kleiner Giulio , Simon Viviana , Edelstein Alexis , Perandones Claudia , Upadhyay Chitra , Lee Benhur , Hioe Catarina E. 
Published: May 2024

IntroductionAntibodies against the SARS-CoV-2 spike protein are a critical immune determinant for protection against the virus. While virus neutralization is a key function of spike-specific antibodies, antibodies also mediate Fc-dependent activities that can play a role in protection or pathogenesis.MethodsThis study characterized serum antibody responses elicited after two doses of heterologous adenovirus-vectored (Ad26/ Ad5) vaccines.ResultsVaccine-induced antibody binding titers and Fc-mediated functions decreased over six months, while neutralization titers remained stable. Comparison of antibody isotypes elicited after Ad26/Ad5 vs. LNP-mRNA vaccination and after infection showed that anti-spike IgG1 were dominant and produced to high levels in all groups. The Ad26/Ad5 vaccines also induced IgG4 but not IgG2 and IgG3, whereas the LNP-mRNA vaccines elicited a full Ig spectrum (IgM, IgG1-4, IgA1-2). Convalescent COVID-19 patients had mainly IgM and IgA1 alongside IgG1. Despite these differences, the neutralization potencies against early variants were similar. However, both vaccine groups had antibodies with greater Fc potencies of binding complement and Fcg receptors than the COVID-19 group. The Ad26/Ad5 group also displayed a greater potency of RBD-specific antibody-mediated cellular phagocytosis.DiscussionAntibodies with distinctive quality were induced by different vaccines and infection. The data imply the utility of different vaccine platforms to elicit antibody responses with fine-tuned Fc activities.
Ampholytic ion-exchange magnetic beads: a promising tool for selecting short fragments in circulating cell-free DNA analysis
Authors: He Gan , Wang Weixuan , Zhou Yongxia , Zhao Guowei , Liao Juan 
Published: May 2024

ObjectiveFor liquid biopsy of cancer, the extraction of circulating cell-free DNA (cfDNA) from plasma is required. We evaluated the efficacy of use of magnetic submicron particles coated with abundant small zwitterions (MSP-ZEWBs) for extracting short fragments of cfDNA.MethodsWe developed and optimized an MSP-ZEWB-based cfDNA extraction method using ampholytic ion-exchange materials and compared its results with those using a control kit. We measured the cfDNA concentration by quantitative polymerase-chain-reaction and using the Qubit method and analyzed cfDNA fragmentation patterns using a bioanalyzer.ResultsThe fragment size of cfDNA isolated from glycine hydrochloric acid at a pH of 2.2 exhibited a better alignment with the DNA marker. The highest DNA intensity was observed at the final concentration of 0.8% polyethylene glycol 8000. The intensity of cfDNA decreased significantly when isolated from plasma with DNA marker using MSP-ZEWBs with an adsorption buffer containing guanidine hydrochloride or isothiocyanoguanidine. All fragments were successfully extracted using MSP-ZEWBs from both plasma and phosphate-buffered saline. Notably, the intensity of short cfDNA fragments isolated using MSP-ZEWBs remained consistent for recovery of long DNA fragments. indicating a potential selective of small fragments.ConclusionThe extraction of plasma cfDNA with MSP-ZEWBs requires no protein denaturation, shows resistance to cells remaining in plasma, and demonstrates higher overall efficiency and better reproducibility than other extraction methods. Use of MSP-ZEWBs may greatly enhance liquid biopsy of cancers through the analysis of plasma cfDNA in clinical practice.