bluuebunny/crossref_metadata_2025_split huggingface dataset:
Scientific research on exotic and native fish farming in Mexico: a sci-entometric view.
Authors: Nieves-Rodríguez Karen N. , Chong-Carrillo Olimpia , Aréchiga-Palomera Martín A. , Peña-Almaraz Omar A. , Palma-Cancino David J. , Vargas-Ceballos Manuel A. , Kelly-Gutiérrez Liza D. , Badillo-Zapata Daniel , Vega-Villasante Fernando 
Published: May 2024

Objective: To analyze through a scientometric study, the research effort in Mexico on the study of native and exotic fish species with aquaculture production technology, indexed in Scopus.
Design/methodology/approach: Fish species were selected based on a previous study and communications with researchers. Scientific articles from 1990 to 2023 were collected from the Scopus database, focusing on studies conducted by Mexican institutions. Data were transferred to Excel® sheets for analysis, including number of publications, topics, institutions, funding sources and open access documents.
Results: The species that shows the greatest number of articles published is Oreochromis niloticus, although there is a clear interest in addressing studies on native species. In general, scientific production is led by institutions such as UNAM, IPN, UABC, CIAD and CIBNOR. The dominant topics are aquaculture, ecology, biochemistry, immunology, and parasitology. CONAHCYT is the main source of funding. About half of the publications were open access.
Limitations on study/implications: The study is limited to the Scopus database, which is the one that includes the largest number of journals worldwide. However, by not including other databases the results could have a slight bias, although we do not consider that the trends changed significantly.
Findings/conclusions: Fish research in Mexico shows a positive outlook, with great interest in several species. However, it faces challenges such as the concentration of effort on exotic species, and the impact of the pandemic on research. A balanced approach is needed to encourage research on native species and facilitate open access to scientific information. This will strengthen fish research in Mexico and enhance its impact on the development of the country.
Development and validation of a high-performance liquid chromatography with tandem mass spectrometry method for quantification of tofacitinib in human plasma
Authors: Vetrova E. S. , Karnakova P. K. , Bagaeva N. S. , Karnakova K. K. , Popova M. O. , Popova A. A. , Archakova O. A. , Komarov T. N. , Shohin I. E. 
Published: May 2024

Introduction. The use of tofacitinib as a pharmacological treatment for rheumatoid arthritis remains relevant in the light of the predicted increase in prevalence of the disease. At the same time, due to the withdrawal of several foreign pharmaceutical companies from the Russian pharmaceutical market, there has been a heightened demand for domestically produced medications, including generic formulations of tofacitinib. Registration of generic drugs necessitates the conduct of bioanalytical studies. Development and validation of a method for quantifying the analyte in biosamples remains to be a crucial part of the bioequivalence studies.Aim. The aim of this research is to develop and validate a method for the quantitative determination of tofacitinib in human plasma using high-performance liquid chromatography as the separation system coupled with a tandem mass spectrometer for detection purposes.Materials and methods. Biosample preparation was based on plasma proteins precipitation using acetonitrile. Baricitinib was selected as an internal standard. The analytical range of the method was 1.00 to 200.00 ng/mL and was further expanded to 0.30 to 200.00 ng/mL during the analytical phase of the study. The mobile phase consisted of water and acetonitrile, both acidified with formic acid (0.1 % v/v). The stationary phase was a Phenomenex Kinetex C18 column [100 × 3.0 mm, with a particle size of 5 µm (Phenomenex, USA)]. Sample separation and detection were carried out using high-performance liquid chromatography coupled with a tandem mass spectrometry (HPLC-MS/MS), operating in positive ion mode. The multiple reaction monitoring (MRM) transitions selected for the analyte and the internal standard were 313.30 to 173.00 m/z and 371.90 to 186.00 m/z, respectively.Results and discussion. The developed assay was validated in accordance with the current requirements of regulatory documentation from the EAEU (Eurasian Economic Union), FDA (US Food and Drug Administration), and EMA (European Medicines Agency) with the following parameters being evaluated: selectivity, specificity, carry-over, matrix effect, recovery, calibration curve, lower limit of quantitation, accuracy, precision, stability. The validated method was applied in the analytical part of a bioequivalence study of domestically produced generic tofacitinib.Conclusion. A method for the quantitative determination of tofacitinib in human blood plasma with an analytical range of 0.30–200.00 ng/mL was developed and validated. Application of the assay during the analytical phase of bioequivalence study of generic tofacitinib confirms the possibility of using the method in similar bioanalytical investigations.
Masculine Virtù and Feminine Virtue in Much Ado About Nothing
Authors: Sutherland Kristina 
Published: May 2024

This chapter discusses the gender aspects of virtues in Much Ado About Nothing, in which the perfection of the Italianate concept of virtù through Claudio, a man of strength and action, and the feminine virtues of chastity, modesty, and obedience via Hero are not enough to guarantee happiness. These qualities become weapons when Don John presents his fictional tableau of an unfaithful Hero, pushing Claudio to save his reputation by sacrificing his bride. To correct this patriarchal imbalance, Shakespeare presents a companionate marriage as he draws together Beatrice and Benedick. A relationship founded on mutual admiration and equality in wit, the play’s second match creates a virtue virtuosa, a new standard of conduct that valorises feminine virtues and excises the violence caused by masculine aggression and male privilege.
Cymbeline and the ‘Swan’s Nest’ of Britain: Insularity, Chastity and Imogen’s Transnational Virtues
Authors: Gadaleto Michael 
Published: May 2024

This chapter considers how Shakespeare links the virtues of Cymbeline’s great heroine, Imogen, with the larger virtues of the British nation; and how both individual and national virtue change over the course of the play, evolving from a traditional and sexist emphasis on purity, chastity and enclosed protectiveness (the island-nation model) into a more vulnerable but bold emphasis on hybridity and engagement with the other (the transnational model). Much as Imogen’s female virtue comes to signify a more dynamic ethic of courage, active fidelity and openness to the larger world, so too does Shakespeare’s play ultimately reject the comfortable insularity of the island-nation model, instead conjuring a thornier but more compelling world in which a distinct ‘British’ identity exists, not in spite of but precisely because of its transnational character and ties – an idea emblematized in Imogen’s famous image of the British nation as “a swan’s nest.”
Single cell RNA-sequencing and RNA-tomography of the avian embryo extending body axis
Authors: Mok G. F. , Turner S. , Smith E. L. , Mincarelli L. , Lister A. , Lipscombe J. , Uzun V. , Haerty W. , Macaulay I. C. , Münsterberg A. E. 
Published: May 2024

Introduction: Vertebrate body axis formation initiates during gastrulation and continues within the tail bud at the posterior end of the embryo. Major structures in the trunk are paired somites, which generate the musculoskeletal system, the spinal cord—forming part of the central nervous system, and the notochord, with important patterning functions. The specification of these different cell lineages by key signalling pathways and transcription factors is essential, however, a global map of cell types and expressed genes in the avian trunk is missing.Methods: Here we use high-throughput sequencing approaches to generate a molecular map of the emerging trunk and tailbud in the chick embryo.Results and Discussion: Single cell RNA-sequencing (scRNA-seq) identifies discrete cell lineages including somites, neural tube, neural crest, lateral plate mesoderm, ectoderm, endothelial and blood progenitors. In addition, RNA-seq of sequential tissue sections (RNA-tomography) provides a spatially resolved, genome-wide expression dataset for the avian tailbud and emerging body, comparable to other model systems. Combining the single cell and RNA-tomography datasets, we identify spatially restricted genes, focusing on somites and early myoblasts. Thus, this high-resolution transcriptome map incorporating cell types in the embryonic trunk can expose molecular pathways involved in body axis development.
Seasonal influence on the essential oil chemical composition of Hyptis crenata Pohl ex Benth.: a valuable plant from Marajó, Brazil
Authors: de Lima Maria Nancy Norat , Santos Paulo Vinicius Lima , Jerônimo Lucas Botelho , Viana Rian Martins , da Silva Joyce Kelly , Setzer William N. , Maia José Guilherme S. , Figueiredo Pablo Luis B. 
Published: May 2024

Introduction: Essential oils (EOs) from the Hyptis genus have been reported as bactericides and fungicides. However, the properties of these oils can be affected by climatic factors, as well as the collection period, which promotes changes in the chemical composition of the oil. In this context, this study aimed to evaluate the climatological influences on the chemical composition of the essential oil from the leaves of Hyptis crenata.Methods: The leaves were collected in Marajó island (Brazil) monthly for a year. The EOs were obtained by hydrodistillation and analyzed by Gas Chromatography coupled to Mass Spectrometry (GC-MS). Pearson’s correlation was used to evaluate the relationship between climatic parameters, content, and chemical composition of essential oil; multivariate analysis was used to evaluate the interrelationship between samples and their chemical constituents.Results and Discussion: The constituents with the highest contents (&gt;2.0%) in essential oils during the studied period were 1,8-cineole (28.48% ± 4.32%), α-pinene (19.58% ± 2.29%), camphor (11.98% ± 2.54%), β-pinene (9.19% ± 1.47%), limonene (6.12% ± 3.15%), α-terpineol (2.42% ± 0.25%) and borneol (2.34% ± 0.48%). β-Pinene significantly correlated (p &lt; 0.05) with precipitation and humidity. According to the chemometric tools, two groups were formed: chemical profile I, marked by 1,8 cineole, α-pinene, β-pinene, borneol, α-terpineol, and limonene, while group II (July) presented a chemical type characterized by camphor. It is understood that the species in question can be a reliable source of biologically active components during different climatic periods in the Amazon. The chemical variability could have significant implications for the pharmaceutical industry and traditional medicine.
Effectiveness of medication self-management, self-monitoring and a lifestyle intervention on hypertension in poorly controlled patients: The MEDICHY randomized trial
Authors: Unda Villafuerte Fabián , Llobera Cànaves Joan , Estela Mantolan Andreu , Bassante Flores Patricia , Rigo Carratalà Fernando , Requena Hernández Ana , Oliver Oliver Bartolomé , Pou Bordoy Joan , Moreno Sancho María Lucía , Leiva Alfonso , Lorente Montalvo Patricia ,   The MEDICHY Group
Published: May 2024

BackgroundUncontrolled hypertension is a common problem worldwide, despite the availability of many effective antihypertensive drugs and lifestyle interventions. We assessed the efficacy of a multi-component intervention in individuals with uncontrolled hypertension in a primary care setting.MethodsThis study was a randomized, multicenter, parallel, two-arm, single-blind controlled trial performed in primary healthcare centers in Mallorca (Spain). All participants were 35 to 75-years-old and had poorly controlled hypertension. Patients were randomly assigned in a 1:1 ratio to a control group (usual care) or an intervention group (self-monitoring of blood pressure, self-titration of hypertensive medications, dietary interventions, and physical activity interventions). The primary outcome was decrease in the mean SBP at 6 months relative to baseline.ResultsA total of 153 participants were randomized to an intervention group (77) or a control group (76). After 6 months, the intervention group had a significantly lower systolic blood pressure (135.1 mmHg [±14.8] vs. 142.7 mmHg [±15.0], adjusted mean difference: 8.7 mmHg [95% CI: 3.4, 13.9], p &lt; 0.001) and a significantly lower diastolic blood pressure (83.5 mmHg [±8.8] vs. 87.00 mmHg [±9.0], adjusted mean difference: 5.4 [95% CI: 2.9, 7.8], p &lt; 0.0001). The intervention group also had significantly more patients who achieved successful blood pressure control (&lt;140/90 mmHg; 54.4% vs. 32.9%, p = 0.011).DiscussionSelf-monitoring of blood pressure in combination with self-management of hypertensive medications, diet, and physical activity in a primary care setting leads to significantly lower blood pressure in patients with poorly controlled hypertension.Clinical Trial Registration:ClinicalTrials.gov, identifier ISRCTN14433778.
Uptake of tire-derived compounds in leafy vegetables and implications for human dietary exposure
Authors: Sherman Anya , Hämmerle Luzian Elijah , Ben Mordechay Evyatar , Chefetz Benny , Hüffer Thorsten , Hofmann Thilo 
Published: May 2024

IntroductionTire and road wear particles are one of the most abundant types of microplastic entering the environment. The toxicity of tire and road wear particles has been linked to their organic additives and associated transformation products. Tire and road wear particles, and associated tire-derived compounds are introduced to the agricultural environment via atmospheric deposition, irrigation with reclaimed wastewater, and the use of biosolids (treated sewage sludge) as fertilizer. In the agricultural environment, these tire-derived compounds could be taken up by edible plants, leading to human exposure.MethodsSixteen tire-derived compounds were measured in twenty-eight commercial leafy vegetable samples from four countries. Based on the results, the estimated daily intake of these tire-derived compounds was calculated due to leafy vegetable consumption based on local diets under a mean and maximum concentration scenario.ResultsIn commercial leafy vegetables, six tire-derived compounds were detected: benzothiazole (maximum concentration—238 ng/g dry weight), 2-hydroxybenzothiazole (maximum concentration—665 ng/g dry weight), 1,3-diphenylguanidine (maximum concentration—2.1 ng/g dry weight), N-(1,3-dimethylbutyl)-N′-phenyl-p-phenylenediamine (6PPD, maximum concentration—0.4 ng/g dry weight), N-Isopropyl-N-phenyl-4-phenylenediamine (IPPD, maximum concentration—0.1 ng/g dry weight), and N-phenyl-N-cyclohexyl-p-phenylenediamine (CPPD, maximum concentration—0.3 ng/g dry weight). At least one compound was present in 71% of samples analyzed. The estimated daily intake for 1,3-diphenylguanidine ranged from 0.05 ng/person/day in the mean scenario to 4.0 ng/person/day in the maximum scenario; benzothiazole ranged from 12 to 1,296 ng/person/day; 6PPD ranged from 0.06 to 2.6 ng/person/day; IPPD ranged from 0.04 to 1.1 ng/person/day; CPPD ranged from 0.05 to 2.6 ng/person/day.DiscussionStatistical analyses did not reveal correlation between known growth conditions and tire-derived compound concentrations in the leafy vegetable samples. The estimated daily intake via leafy vegetable consumption was generally lower than or comparable to the estimated daily intake via other known sources. However, we show that tire-derived compounds are taken up by foodstuff, and exposure might be higher for other produce. Future studies are needed to uncover pathways of tire-derived compounds from road to food, assess the exposure to transformation products, and investigate the biological effects associated with this exposure.
Biogeographic patterns of daily wildfire spread and extremes across North America
Authors: Balik Jared A. , Coop Jonathan D. , Krawchuk Meg A. , Naficy Cameron E. , Parisien Marc-André , Parks Sean A. , Stevens-Rumann Camille S. , Whitman Ellen 
Published: May 2024

IntroductionClimate change is predicted to increase the frequency of extreme single-day fire spread events, with major ecological and social implications. In contrast with well-documented spatio-temporal patterns of wildfire ignitions and perimeters, daily progression remains poorly understood across continental spatial scales, particularly for extreme single-day events (“blow ups”). Here, we characterize daily wildfire spread across North America, including occurrence of extreme single-day events, duration and seasonality of fire and extremes, and ecoregional climatic niches of fire in terms of Actual Evapotranspiration (AET) and Climatic Water Deficit (CWD) annual climate normals.MethodsRemotely sensed daily progression of 9,636 wildfires ≥400 ha was used to characterize ecoregional patterns of fire growth, extreme single-day events, duration, and seasonality. To explore occurrence, extent, and impacts of single-day extremes among ecoregions, we considered complementary ecoregional and continental extreme thresholds (Ecoregional or Continental Mean Daily Area Burned + 2SD). Ecoregional spread rates were regressed against AET and CWD to explore climatic influence on spread.ResultsWe found three-fold differences in mean Daily Area Burned among 10 North American ecoregions, ranging from 260 ha day−1 in the Marine West Coast Forests to 751 ha day−1 in Mediterranean California. Ecoregional extreme thresholds ranged from 3,829 ha day−1 to 16,626 ha day−1, relative to a continental threshold of 7,173 ha day−1. The ~3% of events classified as extreme cumulatively account for 16–55% of total area burned among ecoregions. We observed four-fold differences in mean fire duration, ranging from 2.7 days in the Great Plains to 10.5 days in Northwestern Forested Mountains. Regions with shorter fire durations also had greater daily area burned, suggesting a paradigm of fast-growing short-duration fires in some regions and slow-growing long-duration fires elsewhere. CWD had a weak positive relationship with spread rate and extreme thresholds, and there was no pattern for AET.DiscussionRegions with shorter fire durations had greater daily area burned, suggesting a paradigm of fast-growing short-duration fires in some regions and slow-growing long-duration fires elsewhere. Although climatic conditions can set the stage for ignition and influence vegetation and fuels, finer-scale mechanisms likely drive variation in daily spread. Daily fire progression offers valuable insights into the regional and seasonal distributions of extreme single-day spread events, and how these events shape net fire effects.
Long-term safety and impact of immune recovery in heavily treatment-experienced adults receiving fostemsavir for up to 5 years in the phase 3 BRIGHTE study
Authors: Llibre Josep M. , Aberg Judith A. , Walmsley Sharon , Velez Juan , Zala Carlos , Crabtree Ramírez Brenda , Shepherd Bronagh , Shah Rimi , Clark Andrew , Tenorio Allan R. , Pierce Amy , Du Fangfang , Li Bo , Wang Marcia , Chabria Shiven , Warwick-Sanders Michael 
Published: May 2024

IntroductionFostemsavir is a gp120-directed attachment inhibitor approved for heavily treatment-experienced (HTE) adults with multidrug-resistant HIV-1. We provide detailed week 240 safety results from the BRIGHTE study and evaluate the impact of immune recovery on safety outcomes.MethodsThe phase 3 BRIGHTE trial is ongoing; data for this analysis were collected from the first participant’s first visit (February 23, 2015) through the last participant’s last visit for week 240 (March 22, 2021). Safety endpoints were assessed in participants who received fostemsavir + optimized background therapy. In participants with baseline CD4+ T-cell count &lt;200 cells/mm3, exposure-adjusted adverse event (AE) rates were assessed among subgroups with or without CD4+ T-cell count ≥200 cells/mm3 at any time during 48-week analysis periods through week 192.ResultsThrough a median of 258 weeks (range, 0.14–319) of treatment, discontinuations due to AEs occurred in 30/371 (8%) participants. Serious AEs were reported in 177/371 (48%) participants, including 16 drug-related events in 13 (4%) participants. Thirty-five (9%) deaths occurred, primarily related to AIDS or acute infections. COVID-19–related events occurred in 25 (7%) participants; all resolved without sequelae. Among participants with baseline CD4+ T-cell count &lt;200 cells/mm3, 122/162 (75%) achieved CD4+ T-cell count ≥200 cells/mm3 at week 192. Exposure-adjusted AE rates were markedly lower among participants achieving CD4+ T-cell count ≥200 cells/mm3 at any time vs those sustaining &lt;200 cells/mm3. No new AIDS-defining events were reported after week 48 in participants with CD4+ T-cell count ≥200 cells/mm3.ConclusionsCumulative safety findings through the BRIGHTE 240-week interim analysis are consistent with other trials in HTE participants with advanced HIV-1 and comorbid disease. Reduced rates of AIDS-defining events and AEs were observed in participants with immunologic recovery on fostemsavir-based treatment.Clinical trial numberNCT02362503, https://clinicaltrials.gov/study/NCT02362503.
Dissecting causal relationships between immune cells, plasma metabolites, and COPD: a mediating Mendelian randomization study
Authors: Cao Zhenghua , Wu Tong , Fang Yakun , Sun Feng , Ding Huan , Zhao Lingling , Shi Li 
Published: May 2024

ObjectiveThis study employed Mendelian Randomization (MR) to investigate the causal relationships among immune cells, COPD, and potential metabolic mediators.MethodsUtilizing summary data from genome-wide association studies, we analyzed 731 immune cell phenotypes, 1,400 plasma metabolites, and COPD. Bidirectional MR analysis was conducted to explore the causal links between immune cells and COPD, complemented by two-step mediation analysis and multivariable MR to identify potential mediating metabolites.ResultsCausal relationships were identified between 41 immune cell phenotypes and COPD, with 6 exhibiting reverse causality. Additionally, 21 metabolites were causally related to COPD. Through two-step MR and multivariable MR analyses, 8 cell phenotypes were found to have causal relationships with COPD mediated by 8 plasma metabolites (including one unidentified), with 1-methylnicotinamide levels showing the highest mediation proportion at 26.4%.ConclusionWe have identified causal relationships between 8 immune cell phenotypes and COPD, mediated by 8 metabolites. These findings contribute to the screening of individuals at high risk for COPD and offer insights into early prevention and the precocious diagnosis of Pre-COPD.