bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relationship between the Social Cognition and Object Relations Scale (SCORS) and attachment style in a clinical sample
Authors: Stein Michelle B. , Siefert Caleb J. , Stewart Rosemarie Vala , Hilsenroth Mark J. 
Published: August 2010

AbstractThis present study examined the relationship between the Social Cognition and Object Relations Scale (SCORS) and two measures of adult attachment: the Relationship Questionnaire (RQ) and the Experiences in Close Relationships Questionnaire‐Revised (ECR‐R). Forty‐five patients (76% female) at a university‐based outpatient treatment clinic participated in this study. We hypothesized that higher levels of attachment security would be associated with higher, more adaptive ratings on the SCORS variables. Results indicated that the SCORS Self‐Esteem (SE) variable was significantly positively related to the RQ's Secure Attachment ratings and negatively related with the ECR‐R's Anxious Attachment scale. Additionally, negative trends were noted between SE and the RQ's Fearful and Preoccupied Attachment scores. The SCORS Emotional Investments in Relationships and Affective Quality of Representations variables were associated with higher Secure scores and lower, more maladaptive Preoccupied scores on the RQ. It was also associated with greater attachment anxiety as measured by the ECR‐R. Using both clinician (SCORS) and participant‐rated measures (ECR‐R and RQ), this study provides further understanding on how object representations and attachment style relate within a clinical sample. Results are discussed in light of prior research examining relationships between object relations and adult attachments, and clinical implications are also reviewed. Copyright © 2010 John Wiley & Sons, Ltd.Key Practitioner Message:• Individuals with higher levels of attachment anxiety may enter therapy with more self‐image problems.• Individuals with higher levels of attachment anxiety may enter therapy with more maladaptive expectations about relationships.• Patients who endorse high levels of attachment anxiety (e.g., fearful and preoccupied) may be more likely to present with Axis II complaints.• Examining a patient's attachment style and object relations using different measures of assessment (e.g., explicit and implicit) can help gain a deeper and more comprehensive understanding of a patient.
Structural, thermal and optical studies on 2‐naphthol crystals
Authors: Guru Prasad L. , Krishnakumar V. , Shanmugam G. , Nagalakshmi R. 
Published: August 2010

AbstractSingle crystals of 2‐naphthol was grown in slow evaporation solution growth technique. Grown crystals were characterized by various characterization techniques. Powder X‐ ray diffraction studies reveal that the grown crystal belongs to monoclinic system with noncentrosymmetric space group. Vibrational bands of the various functional groups and their significance were investigated by analyzing the vibrational spectra. Melting point and the decomposition temperature of the grown crystal was premeditated from the thermal analysis. From the UV‐VIS‐NIR spectrum the electronic excitation mechanism and the transmittance abilities of the crystal was studied. Theoretical value of first static hyperpolarizability was calculated and compared with urea. Second harmonic generation efficiency of the crystal is 0.6 times that of KDP. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Universal basis for ranking small‐signal aspects of compensation techniques for operational amplifiers
Authors: Cherry Edward M. 
Published: August 2010

AbstractA multi‐pole gain‐bandwidth theorem sets an upper bound to the bandwidth that can be achieved with specified DC gain and external load capacitance, for a given selection of transistors and operating points. A product of the poles and zeros is constrained by ∏(gm/C) evaluated over the forward‐path active devices. Most practical compensation techniques degrade the actual bandwidth, by factors which the paper explores in detail; depending on the circuit topology, some compensating capacitors can add to the intrinsic device capacitances. A few techniques achieve the ideal. Copyright © 2010 John Wiley & Sons, Ltd.
Multifunctional Nanocomposite Thin Films by Aerosol‐Assisted CVD
Authors: Warwick Michael E.A. , Dunnill Charles W. , Binions Russell 
Published: August 2010

AbstractThe aerosol‐assisted (AA)CVD reaction of titanium isopropoxide and a solution of preformed tin dioxide nanoparticles leads to the production of titanium dioxide/tin dioxide nanocomposite thin films on glass substrates. Scanning electron microscopy (SEM) studies show that film composition and morphology are linked, with larger island sizes being observed as more tin dioxide nanoparticles are incorporated into the films. Reflectance measurements performed on the nanocomposite thin films show an increase in reflection in the infrared (IR) portion of the spectrum with increased tin dioxide nanoparticle incorporation. Photocatalysis experiments indicate that the nanocomposite films are photo‐active with irradiation by 365 nm light. The nanocomposite thin films display both low‐emissivity and self‐cleaning functionalities; and as such they are multifunctional.
Investigation of Atmospheric Pressure CVD‐Produced Aluminum Coatings for the Protection of Steel from Corrosion
Authors: Gu Sipeng , Maeng Sungmin , Suh Yongseok , Levy Roland A. , Deavenport Dennis L. , Gómez Fernando J. , Newberg Samuel S. , Brooman Eric W. , Berman Elizabeth S. , Kleek John J. , Beatty John H. , Schwartz Andrew S. , Gaydos Stephen P. 
Published: August 2010

AbstractThis study investigates the use of atmospheric pressure (AP)CVD‐produced aluminum (Al) coatings for the protection of steel from corrosion. Coatings produced through thermal decomposition of a triisobutylaluminum (TIBAL)‐based precursor at 548 and 573 K are shown to exhibit growth rates of 0.5 and 1.1 µm min−1, respectively. Glow discharge optical emission spectroscopy (GDOES) reveals that, in the main, the coatings consist of pure Al with oxygen present as a minor constituent. Results of the structural, morphological, electrical, and mechanical characterization reveal that the APCVD Al coatings exhibit a face center cubic (fcc) structure with no preferred orientation, a non‐columnar grain structure with grain size ranging from 3 to 10 µm, and a surface roughness close to 2 µm. Such coatings are also shown to offer an electrical resistivity of 3.5 µΩ·cm, a density of 2.60 g cm−3, and an adhesive strength of 703 kg cm−2. Cross‐sectional examination of coated samples indicate that the APCVD process can yield excellent step coverage and throwing power. Hydrogen embrittlement (HE) tests reveal that Al‐coated bars with no post baking experience premature failure due to the presence of incorporated reaction product, hydrogen, in the steel substrate. Samples post‐baked at 463 K for 23 h to remove the hydrogen from the substrate are shown to withstand the load for as long as 202 h. Corrosion studies conducted using potentiodynamic polarization measurements, electrochemical impedance spectroscopy, and salt fog tests show that the corrosion resistance of the APCVD Al coatings is comparable to that of pure Al foils, and that such coatings provide acceptable sacrificial protection for steel substrates.
A simple method to sort ESC‐derived adipocytes
Authors: Schaedlich Kristina , Knelangen Julia M. , Navarrete Santos Alexander , Fischer Bernd , Navarrete Santos Anne 
Published: August 2010

AbstractBecause of the increasing incidence of worldwide obesity, cell culture models which enable the study of adipose tissue development are of particular importance. The murine embryonic stem cell (ESC) line CGR8 differentiates into adipocytes with a differentiation efficiency of up to 15%. A critical step for the analysis of stem cell‐derived adipogenesis is the reliable separation of adipocytes. Here we report on how to (i) gently separate the cells of embryoid bodies (EBs) and (ii) identify and sort adipocytes from the rest of the heterogeneous cell mixture. Up to the present, no adipocyte specific surface marker is known for fluorescence activated cell sorting (FACS). After separation we employed two independently existing FACS methods for adipocyte cell sorting. These methods are based on Nile red staining and granularity. For stem cell‐derived adipocytes only the combination of both methods led to a reliable, efficient, and highly reproducible FACS analysis, as shown by the presence and absence of adipocyte specific markers in positively and negatively sorted cells. © 2010 International Society for Advancement of Cytometry
Flow cytometric analysis of CD123 is useful for immunophenotyping classical Hodgkin lymphoma
Authors: Fromm Jonathan R. 
Published: August 2010

AbstractAlthough the diagnosis of classical Hodgkin lymphoma (CHL) is traditionally made morphologically in tissue sections, it is now possible to diagnose CHL by flow cytometry (FC). In an effort to identify additional antigens on Hodgkin and Reed‐Sternberg (HRS) cells that might be useful for immunophenotyping this lymphoma by FC, we examined the expression of CD123 (α chain of the IL‐3R) on HRS cells and compared this with the expression of CD123 in other lymph node samples (372 tissue specimens, including 98 reactive and 274 neoplastic cases), using a nine‐color FC reagent combination including anti‐CD123. CD123 was found to be expressed on the majority of HRS cell populations (59% of 59 CHL lymph nodes examined), rarely on B‐cell non‐Hodgkin lymphomas (NHLs) (3 of 3 hairy cell leukemia cases, 3 of 29 chronic lymphocytic leukemia/small lymphocytic lymphoma (CLL/SLL) cases, 1 of 55 large B‐cell lymphoma cases, but not on 95 other B‐cell NHL examined), and in none of the 16 T‐cell NHL cases examined. In reactive lymph nodes, CD123 expression was largely limited to plasmacytoid dendritic cells and rare histiocyte populations that can readily be distinguished from HRS populations. As the expression of this antigen is shown to be relatively limited in B‐ and T‐NHL and in reactive lymph node populations, assessment of CD123 expression is useful for supporting the FC diagnosis of CHL. © 2010 International Clinical Cytometry Society
Performance improvement of fixed wireless access networks by conjunction of dual polarization and time domain radio resource allocation technique
Authors: Vavoulas Alexander , Vaiopoulos Nicholas , Varoutas Dimitris A. , Chipouras Aris , Stefanou George 
Published: August 2010

AbstractThis paper considers the impact of dual polarization adoption on the performance for a fixed wireless access (FWA) network. The major limiting factor of the performance in these systems is co‐channel interference (CCI) originating from intracell and intercell concurrent transmissions. The proposed framework combines an appropriate time domain radio resource allocation technique with a dual polarization pattern to mitigate CCI and enhance the obtained signal to interference plus noise ratio (SINR). Simulation results present the performance of the proposed framework against various terrain categories and sector antenna characteristics. Copyright © 2010 John Wiley & Sons, Ltd.
Adam10 is essential for early embryonic cardiovascular development
Authors: Zhang Chi , Tian Lei , Chi Congwu , Wu Xiaohui , Yang Xiao , Han Min , Xu Tian , Zhuang Yuan , Deng Kejing 
Published: August 2010

AbstractNotch pathway has been demonstrated to regulate cardiovascular development. One important step in Notch pathway is the cleavage of Notch receptor, during which an intracellular fragment of Notch protein is released to activate downstream genes. It is still uncertain whether Adam10, the mammalian homologue of Kuzbanian in Drosophila, is required to activate the Notch pathway during cardiovascular development. To further understand the physiological function of Adam10 in vascular and cardiac development, we generated mice lacking the Adam10 gene primarily in the endothelial compartment. We found that disruption of Adam10 in endothelial cells resulted in embryonic death after embryonic day 10.5 due to multiple cardiac and vascular defects similar to Notch1 mutants. We further showed that the expression of Notch target genes Snail and Bmp2 are impaired in Adam10‐deficient cardiac tissues. Finally, we provide experimental evidence to support that Adam10 functions in a cell autonomous manner during mammalian cardiac development. Developmental Dynamics 239:2594–2602, 2010. © 2010 Wiley‐Liss, Inc.
Synthesis and Electrochemical Study of an Original Copper(II)‐Capped Salen–Cyclodextrin Complex
Authors: Deunf Elise , Zaborova Elena , Guieu Samuel , Blériot Yves , Verpeaux Jean‐Noël , Buriez Olivier , Sollogoub Matthieu , Amatore Christian 
Published: August 2010

AbstractA new metallocapped cyclodextrin (CD) was synthesized by the regioselective debenzylation, induced by diisobutylaluminium hydride (DIBAL‐H), of perbenzylated cyclodextrins. This reaction allowed for the efficient preparation of an unprecedented CD–salen type copper(II) complex. The electrochemical behavior of both the bound and unbound CD–salen compounds was investigated by cyclic voltammetry. Notably, it was shown that the presence of tert‐butyl groups at the ortho‐ and para‐positions of the salen aromatic rings stabilized the copper(II) phenoxyl radical species that was generated upon the one‐electron oxidation of the starting compound. Importantly, this stabilization remained effective when the salen‐type ligand was covalently attached to the CD. This allowed for investigations of the reactivity of the copper(II) phenoxyl radical complex towards a primary alcohol to be performed by cyclic voltammetry. This reaction can be considered as mimicking the behavior of galactose oxidase. However, under these conditions, no reactivity was observed in the presence of benzyl alcohol. This may be due to distortion, either of the initially square planar salen ligand after its grafting to the CD primary face, and/or of the CD itself. On the other hand, the electrochemical reduction of the un‐grafted copper(II) salen‐type ligand led to a transient anionic species that exhibited significant stability on the time‐scale of the slow cyclic voltammetry measurement in the absence of the CD, but was unstable in the presence of the CD. In the latter case, it was demonstrated that the anionic species was protonated by the CD. Importantly, this protonation was not fast enough to prevent catalytic activation of iodomethane by the electro‐generated copper(I)‐capped salen CD complex.
A Combined Experimental and Density Functional Theory Study on the Pd‐Mediated Cycloisomerization of o‐Alkynylnitrobenzenes – Synthesis of Isatogens and Their Evaluation as Modulators of ROS‐Mediated Cell Death
Authors: Ramana Chepuri V. , Patel Pitambar , Vanka Kumar , Miao Benchun , Degterev Alexei 
Published: August 2010

AbstractHighly selective cycloisomerization of o‐alkynylnitrobenzenes, leading to isatogens, has been achieved by employment of a PdII complex. This reaction is very general and functional‐group‐tolerant. The possible mechanism of this reaction was investigated with the help of DFT calculations. Three possible pathways – namely, the addition of the nitro group either in (i) 5‐exo‐dig or (ii) 6‐endo‐dig mode and (iii) halopalladation – and subsequent intramolecular events have been considered and studied in detail. These investigations revealed that pathway (i) is the favored route to isatogen formation. A preliminary screening of the available isatogens reveals the 2‐alkylisatogens to be novel ROS scavengers capable of inhibiting cellular necroptosis.
Electrocatalytic Reduction of Peroxy Disulfate on the Palladized Aluminum Electrode Modified by Prussian Blue: Application to the Analysis of Decolorizing Powders
Authors: Pournaghi‐Azar M. H. , Ahour F. 
Published: August 2010

AbstractThe mechanism of catalytic reduction of peroxydisulfate on the palladized aluminum electrode modified by Prussian blue (PB/Pd‐Al) was studied. The charge transfer‐rate limiting step as well as overall reduction reaction of S2O82− is found to be a one‐electron and two‐electron abstraction respectively. The modified electrode is exploited for the hydrodynamic amperometry of peroxydisulfate. It is found that the calibration graph is linear in the S2O82−concentration range 5×10−6–1.5×10−3 mol L−1. The detection limit of the method was 2.4×10−6 mol L−1 S2O82. The method was successfully used for the determination of S2O82− in decolorizing powders
Silica‐Polyaniline Based Bienzyme Cholesterol Biosensor: Fabrication and Characterization
Authors: Manesh Kalayil Manian , Santhosh Padmanabhan , Gopalan Anantha Iyengar , Lee Kwang‐Pill 
Published: August 2010

AbstractIn the present investigation, silica‐polyaniline based bienzyme cholesterol biosensor is fabricated through a simple one‐step electrochemical method. The one‐step fabrication process involves electrochemical polymerization of N[3‐(trimethoxysilyl)propyl]aniline to result poly(N[3‐(trimethoxysilyl)propyl]aniline) (PTMSPA) and simultaneous immobilization of two enzymes, horseradish peroxidase (HRP) and cholesterol oxidase (ChOx) into PTMSPA matrix. The modified electrode is designated as PTMSPA‐HRP/ChOx‐ME. PTMSPA facilitates direct electron transfer between the electrode surface and the active redox centers of HRP. This enables the operation of a biosensor at a low working potential of about −150 mV (vs. Ag/AgCl) for the detection of hydrogen peroxide. The PTMSPA‐HRP/ChOx‐ME demonstrates excellent analytical performance for the detection of cholesterol between 1 and 25 mM with high sensitivity and selectivity. PTMSPA possesses features suited for the fabrication of third‐generation biosensors.
Fabrication of a microfluidic Ag/AgCl reference electrode and its application for portable and disposable electrochemical microchips
Authors: Zhou Jianhua , Ren Kangning , Zheng Yizhe , Su Jing , Zhao Yihua , Ryan Declan , Wu Hongkai 
Published: August 2010

AbstractThis report describes a convenient method for the fabrication of a miniaturized, reliable Ag/AgCl reference electrode with nanofluidic channels acting as a salt bridge that can be easily integrated into microfluidic chips. The Ag/AgCl reference electrode shows high stability with millivolt variations. We demonstrated the application of this reference electrode in a portable microfluidic chip that is connected to a USB‐port microelectrochemical station and to a computer for data collection and analysis. The low fabrication cost of the chip with the potential for mass production makes it disposable and an excellent candidate for real‐world analysis and measurement. We used the chip to quantitatively analyze the concentrations of heavy metal ions (Cd2+ and Pb2+) in sea water. We believe that the Ag/AgCl reference microelectrode and the portable electrochemical system will be of interest to people in microfluidics, environmental science, clinical diagnostics, and food research.
Polymeric nanopillar arrays for cell traction force measurements
Authors: Kuo Chiung‐Wen , Shiu Jau‐Ye , Chien Fan‐Ching , Tsai Shih‐Min , Chueh Di‐Yan , Chen Peilin 
Published: August 2010

AbstractThis paper reports the development of a novel force measurement device based on polymeric nanopillar arrays. The device was fabricated by a process combining nanosphere lithography, oxygen plasma treatment, deep etching and nano‐molding. Well‐ordered polymeric nanopillar arrays with various diameters and aspect ratios were fabricated and used as cell culture substrates. Cell traction forces were measured by the deflection of the nanopillars. Since the location of the nanopillars can be monitored at all times, this device allows for the measurement of the evolution of adhesion forces over time.
Systematic engineering of microorganisms to improve alcohol tolerance
Authors: Jia Kaizhi , Zhang Yanping , Li Yin 
Published: August 2010

AbstractMicrobial production of alcohol is a promising approach for obtaining important chemicals and fuels from renewable carbon resources. One of the key problems associated with the fermentative production of alcohol is alcohol toxicity and/or growth inhibition, resulting in a low alcohol titer in the fermentation broth. Cost‐effective production of alcohol needs the scientific community to engineer microbes for increased alcohol tolerance. Here, based on the understanding of the mechanisms of alcohol toxicity and alcohol stress resistance, we review the recent progress in engineering microbial alcohol tolerance by rational approaches. In addition, random approaches including traditional mutagenesis and selection, evolution engineering, random knockout and overexpression libraries, genome shuffling, replicational/transcriptional/translational engineering were also included into this review.
Genotoxicity testing: Moving beyond qualitative “screen and bin” approach towards characterization of dose‐response and thresholds
Authors: Pottenger Lynn H. , Gollapudi B. Bhaskar 
Published: August 2010

AbstractFor more than 40+ years, genotoxicity data have been interpreted in a qualitative, binary mode; a chemical is considered either positive or negative for a response in the test system. Although dose–response information is sometimes used in this decision, it is not routine to obtain the amount of information needed to inform risk assessment, for example to determine no‐observed‐genotoxic‐effect‐levels, primarily due to the historical view of genotoxic responses as “linear, no‐threshold.” Only recently have researchers begun to address this issue through robust experimental designs and application of statistical models. A growing body‐of‐evidence supports the existence of response thresholds for a number of mutagenic agents, in vitro and in vivo. Clearly, simple observation of a “hockey‐stick” dose–response curve is not sufficient to establish a threshold. Collection of robust empirical data must be supported with an analysis of biological plausibility for the observed threshold. In this context, a chemical‐specific mode‐of‐action (MOA) approach, which identifies key events responsible for the observed mutagenic effect, is extremely valuable. Biomarkers of key events, providing qualitative and quantitative information, can be integrated in a weight‐of‐evidence‐based assessment of genotoxicity data from multiple test systems and used to identify data gaps to resolve/reduce uncertainties during the risk assessment process. To this end, specific recommendations on study design and data analysis are proposed. As the Environmental Mutagen Society celebrates its 40th anniversary, the field of genetic toxicology is marking a milestone on the path to a new paradigm, using a MOA, data‐driven approach to answer questions about thresholds for genotoxic agents. Environ. Mol. Mutagen., 2010. © 2010 Wiley‐Liss, Inc.