bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multiomics Analysis of Disulfidptosis Patterns and Integrated
Machine Learning to Predict Immunotherapy Response in Lung
Adenocarcinoma
Authors: Liu Junzhi , Li Huimin , Zhang Nannan , Dong Qiuping , Liang Zheng 
Published: April 2024

Background:
Recent studies have unveiled disulfidptosis as a phenomenon intimately
associated with cellular damage, heralding new avenues for exploring tumor
cell dynamics. We aimed to explore the impact of disulfide cell death on the tumor immune
microenvironment and immunotherapy in lung adenocarcinoma (LUAD).

Methods:
We initially utilized pan-cancer transcriptomics to explore the expression,
prognosis, and mutation status of genes related to disulfidptosis. Using the LUAD multi-
-omics cohorts in the TCGA database, we explore the molecular characteristics of subtypes
related to disulfidptosis. Employing various machine learning algorithms, we construct
a robust prognostic model to predict immune therapy responses and explore the
model's impact on the tumor microenvironment through single-cell transcriptome data.
Finally, the biological functions of genes related to the prognostic model are verified
through laboratory experiments.

Results:
Genes related to disulfidptosis exhibit high expression and significant prognostic
value in various cancers, including LUAD. Two disulfidptosis subtypes with distinct
prognoses and molecular characteristics have been identified, leading to the development
of a robust DSRS prognostic model, where a lower risk score correlates with a
higher response rate to immunotherapy and a better patient prognosis. NAPSA, a critical
gene in the risk model, was found to inhibit the proliferation and migration of LUAD
cells.

Conclusion:
Our research introduces an innovative prognostic risk model predicated upon
disulfidptosis genes for patients afflicted with Lung Adenocarcinoma (LUAD). This
model proficiently forecasts the survival rates and therapeutic outcomes for LUAD patients,
thereby delineating the high-risk population with distinctive immune cell infiltration
and a state of immunosuppression. Furthermore, NAPSA can inhibit the proliferation
and invasion capabilities of LUAD cells, thereby identifying new molecules for clinical
targeted therapy.
Chemistry, Analysis, and Biological Aspects of Daprodustat, A New Hypoxia Inducible Factor Prolyl Hydroxylase Inhibitor: A Comprehensive Review
Authors: Patil Roshani , Sharma Sanjay 
Published: April 2024

Background:
The National Health and Nutrition Examination Survey (NHANES) carried
out a survey between 2007-10 and found that as compared to the general population, the prevalence
of anemia in chronic kidney disease (CKD) patients was twice high. Daprodustat is an investigational
novel drug for the treatment of renal anemia.

Objective:
The objective of this study is to provide a comprehensive review of chemistry, synthesis,
pharmacology, pharmacokinetic, and bioanalytical methods for the analysis of Daprodustat.

Methods:
To improve understanding, a review was carried out by creating a database of relevant
prior research from electronic sources such as ScienceDirect and PubMed. The methodology is
shown in the flowchart of the literature selection process.

Results:
The drug was approved in 2020 for therapeutic purposes in Japan. It is a novel drug approved
for the treatment of anemia in chronic kidney disease for oral administration. It is intended
for adults who have undergone dialysis for a minimum of four months and are experiencing anemia
as a result of chronic kidney disease.

Conclusion:
This review examines therapeutic, pharmacological, and analytical aspects related to
the novel drug Daprodustat.
Exploring the Antitumor Efficacy of PEGylated Liposomes Loaded with Licorice Extract for Cancer Therapy
Authors: Haghighat Zeinab Azizi , Safekordi Aliakbar , Ardjmand Mehdi , Akbarzadeh Azim 
Published: April 2024

Background:
Glycyrrhizic Acid (GA), a compound derived from licorice, has exhibited
promising anticancer properties against several cancer types, including Prostate Cancer (PCa)
and Gastric Cancer (GCa).

Objective:
This study has introduced a novel approach involving the encapsulation of GA and Licorice
extract (Lic) into Polyethylene Glycol Liposomes (PEG-Lip) and assessed their efficacy
against AGS (human gastric cancer) and PC-3 (human prostate cancer) cells, marking the first report
of this endeavor.

Methods:
We synthesized GA-loaded PEG-Lip (GA PEG-Lip) and Lic-loaded PEG-Lip (Lic
PEG-Lip) through the reverse-phase evaporation method.

Results:
Characterization of these liposomal formulations revealed their size, drug encapsulation,
and loading efficiencies to be 110 ± 2.05 nm, 117 ± 1.24 nm; 61 ± 0.81%, 34 ± 0.47%; and 8 ±
0.41% and 4.6 ± 0.21%, respectively. Importantly, the process has retained the chemical structure
of both GA and Lic. Furthermore, GA and Lic have been released from the PEG-Lip formulations
in a controlled manner.
In our experiments, both nanoformulations exhibited enhanced cytotoxic effects against AGS and
PC-3 cells. Notably, GA PEG-Lip outperformed Lic PEG-Lip, reducing the viability of PC-3 and
AGS cells by 12.5% and 15.9%, respectively

Conclusion:
These results have been corroborated by apoptosis assays, which have demonstrated
GA PEG-Lip and Lic PEG-Lip to induce stronger apoptotic effects compared to free GA and Lic
on both PC-3 and AGS cells.
This study has underscored the potential of encapsulating GA and Lic in PEG-Lip as a promising
strategy to augment their anticancer efficacy against prostate and gastric cancers.
Electrochemical Modification of Metronidazole and its Application as Antibacterial and Potential Drug Agent
Authors: Egu Samuel Attah , Idih Sunday Okpanachi , Idih Favour Moses , Aminu Ruth Foluke , Achimugu Lawrence , Omale Aminu , Omale Jamila Audu , Abah Lian Ojotule , Amlabu Emmanuel 
Published: April 2024

Background:
Drug resistance poses a threat to global health given the disturbing rate at
which microbiological illnesses are rising and the widespread use of antibiotics. While naturally
occurring antibiotics reduce the likelihood of bacterial resistance, recognized drugs can develop the
same properties when structurally modified. Metronidazole has been targeted to achieve this feat.

Objective:
The purpose of this work is to enable the structural modification of metronidazole by
means of electric current, using inexpensive, easily accessible magnesium ribbon as electrodes in
order to produce a novel, effective antibiotic.

Methods:
With magnesium ribbon electrodes, metronidazole is modified electrochemically, in an
undivided cell system. Ultraviolet-visible (UV-Vis), Fourier Transform Infrared (FTIR), and Gas
Chromatography-Mass Spectrometry (GC-MS) were used to characterize the product. Antibacterial
activity was evaluated using the agar well diffusion method and Wistar rats were used for in vivo
toxicity assessment.

Results:
2-Methyl-1-vinyl-1H-imidazol-5-amine showed good antibacterial activities compared to the
standard nitrofurantoin and toxicity evaluations using Wistar rats revealed that the product might
induce dose-dependent variations in kidney function biomarkers, with doses of 100 mg/kg and below.
However, when the compound was administered orally, there was no significant effect on liver
function, even at a dose of 1000 mg/kg.

Conclusion:
These results point to the modified metronidazole's potential as a potent antibiotic with
controllable toxicity, indicating that additional research into its pharmacological uses is necessary.
Use and Efficacy of I-gel in Complicated Intubations in Newborn Infants: A Review of Case Reports in the Literature
Authors: Barbieri Emiliano , Giordano Maurizio , Sorgente Giuseppe , Borgia Giulia , Improta Marta , Colonna Monica , Crisci Valeria , Fattorusso Valentina , Barbieri Flavia , Buonocore Giuseppe , De Bernardo Giuseppe 
Published: April 2024

Introduction:
Current guidelines for neonatal resuscitation suggest the use of a laryngeal
mask when ventilation with both facemask and endotracheal tube has failed in newborns
weighing >2000 g or delivered ≥ 34 weeks of gestation age. Paediatric I-gel® is one of the latest
supraglottic airway management devices suitable for children and newborns. I-gel® use was effective
in guaranteeing adequate ventilation in patients with anatomic abnormalities in case of respiratory
impairment or during surgical procedures after the induction of anaesthesia.

Objective:
The purpose of our review was to evaluate the use and efficacy of I-gel® in case of complicated
intubations.

Methods:
In July 2023, two authors of this paper independently conducted searches of the MEDLINE,
Web of Science, and Scopus databases without imposing any time constraints or other restrictions.
Three case reports were included, each describing the use of I-gel® device in difficult intubations
in newborns with anatomical abnormalities.

Results:
No difficulties were reported in the insertion of the device, which was placed even by inexperienced
clinicians

Conclusion:
The data collected highlighted the possibility of using I-gel® not only as a rescue device
after attempted and failed endotracheal placement but also as a first choice in selected patients.
Studies on larger cohorts would be needed. Further research involving larger patient cohorts
of multicentre NICUs is necessary to confirm the use of laryngeal masks in neonates weighing
less than 2000 grams.
Medicinal Plants in the Treatment of Myocardial Infarction Disease:
A Systematic Review
Authors: Rathore Anamika , Sharma Anuj Kumar , Murti Yogesh , Bansal Sonal , Kumari Vibha , Snehi Varsha , Kulshreshtha Mayank 
Published: April 2024

Background:
Myocardial infarction (MI), also referred to as a "heart attack," is
brought on by a partial or total interruption of blood supply to the myocardium. Myocardial infarction can be "silent," go undiagnosed, or it can be a catastrophic occurrence that results in
hemodynamic decline and untimely death. In recent years, herbal remedies for MI have become
effective, secure, and readily accessible.

Objective:
The purpose of this review was to examine the medicinal plants and phytochemicals
that have been used to treat MI in order to assess the potential contribution of natural substances
to the development of herbal MI treatments.

Methodology:
A literature search was employed to find information utilizing electronic databases, such as Web of Science, Google Scholar, PubMed, Sci Finder, Reaxys, and Cochrane.

Results:
The identification of 140 plants from 12 families led to the abstraction of data on the
plant families, parts of the plant employed, chemical contents, extracts, model used, and dose.

Conclusion:
The majority of the MI plants, according to the data, belonged to the Fabaceae
(11%) and Asteraceae (9%) families, and the most prevalent natural components in plants with
MI were flavonoids (43%), glucosides (25%), alkaloids (23%), phenolic acid (19%), saponins
(15%), and tannins (12%).
Targeting Insulin- and Calcium-related Pathways for Potential Treatments
for Alzheimer's Disease and Diabetes
Authors: Sood Shreya , Devi Sushma , Singh Thakur Gurjeet , Singh Randhir 
Published: April 2024

:
Alzheimer's disease and diabetes are common disorders among the elderly population
and have emerged as a major health concern. Both diseases pose considerable risks to one another.
Diabetics have a significantly increased probability of getting Alzheimer's disease
throughout their lifetime. These diseases are linked because, both share common risk factors
such as impaired carbohydrate metabolism, insulin resistance, oxidative stress, inflammatory
response, mitochondrial dysfunction, and amyloidosis. Insulin is a vital hormone responsible for
bringing extremely high glucose levels back to normal and its receptors available in the hippocampus
help in enhancing cognitive function. Insulin resistance consequently serves as a link
between both diabetes and AD. Similarly, amylin is another hormone secreted by the pancreas
along with insulin. During diabetes, amylin gets oligomerized and forms a neurotoxic complex
with Aβ inside the brain, which causes AD to develop. Along with these, another main mechanism
influencing AD development is Ca2+ dyshomeostasis. Insulin production from the pancreas
is generally aided by Ca2+, but in excess, it can cause dysregulation of many signaling pathways
such as CaMKK2, CAMP, CREB, MAPK, STIM\Orai, etc. which can ultimately result in
the pathogenesis of AD in diabetic people. In this review, we discussed in detail the pathogenesis
of AD associated with diabetes and the mechanisms initiating their progression.
Improving Tamoxifen Performance in Inducing Apoptosis and Hepatoprotection by Loading on a Dual Nanomagnetic Targeting System
Authors: Zhao Yanfang , Ding Wanbao , Zhang Peixian , Deng Lei , Long Yi , Lu Jiuqin , Shiri Fereshteh , Heidari Majd Mostafa 
Published: April 2024

Background:Although tamoxifen (TMX) belongs to selective estrogen receptor modulators (SERMs) and selectively binds to estrogen receptors, it affects other estrogen-producing tissues due to passive diffusion and non-differentiation of normal and cancerous cells and leads to side effects.Methods:The problems expressed about tamoxifen (TMX) encouraged us to design a new drug delivery system based on magnetic nanoparticles (MNPs) to simultaneously target two receptors on cancer cells through folic acid (FA) and hyaluronic acid (HA) groups. The mediator of binding of two targeting agents to MNPs is a polymer linker, including dopamine, polyethylene glycol, and terminal amine (DPN).Results:Zeta potential, dynamic light scattering (DLS), and Field emission scanning electron microscopy (FESEM) methods confirmed that MNPs-DPN-HA-FA has a suitable size of ~105 nm and a surface charge of -41 mV, and therefore, it can be a suitable option for carrying TMX and increasing its solubility. The cytotoxic test showed that the highest concentration of MNPs-DPN-HA-FA-TMX decreased cell viability to about 11% after 72 h of exposure compared to the control. While the protective effect of modified MNPs on normal cells was evident, unlike tamoxifen, the survival rate of liver cells, even after 180 min of treatment, was not significantly different from the control group. The protective effect of MNPs was also confirmed by examining the amount of malondialdehyde, and no significant difference was observed in the amount of lipid peroxidation caused by modified MNPs compared to the control. Flow cytometry proved that TMX loaded onto modified MNPs can induce apoptosis by targeting the overexpressed receptors on cancer cells. Real-time PCR showed that the modified MNPs activated the intrinsic and extrinsic mitochondrial pathways of apoptosis, so the Bak1/Bclx ratio for MNPs-DPN-HAFA- TMX and free TMX was 70.82 and 0.38, respectively. Also, the expression of the caspase-3 gene increased 430 times compared to the control. On the other hand, only TNF gene expression, which is responsible for metastasis in some tumors, was decreased by both free TMX and MNPs-DPN-HA-FA-TMX. Finally, molecular docking proved that MNPs-DPN-HA-FA-TMX could provide a very stable interaction with both CD44 and folate receptors, induce apoptosis in cancer cells, and reduce hepatotoxicity.Conclusion:All the results showed that MNPs-DPN-HA-FA-TMX can show good affinity to cancer cells using targeting agents and induce apoptosis in metastatic breast ductal carcinoma T-47D cell lines. Also, the protective effects of MNPs on hepatocytes are quite evident, and they can reduce the side effects of TMX.
Ionic Liquids Immobilized Synthesis of New Xanthenes Derivatives and their
Antiproliferative, Molecular Docking, and Morphological Studies
Authors: Mohareb Rafat M. , Ibrahim Rehab A. , Al Farouk Fatma O. , Alwan Ensaf S. 
Published: April 2024

Background:
Xanthenes and benzoxanthenesare are highly valuable compounds in organic chemistry
and medicinal chemistry. Xanthene derivatives were found to have many applications in medicinal chemistry.

Objective:
This work aims to explore the synthesis of xanthene derivatives with various substituents and find the
possibility of their uses as anticancer agents.

Methods:
The basic starting compound through this work was the 2,3-dihydro-1H-xanthen-1-one (3), which was
synthesized from the reaction of cyclohexan-1,3-dione and 2-hydroxybenzaldehyde. Compound 3 was used to
synthesize new thiophene, pyrimidine, isoxazole, and thiazole derivatives based on the xanthenes nucleus. Fused
xanthene derivatives were obtained through further heterocyclization reactions. Multicomponent reactions expressed
in this work were carried out in the presence of solvent catalyzed by Et3N and in solvent-free ionic liquid
immobilized catalyst.

Results:
Cytotoxicity for the newly synthesized compounds toward cancer cell lines was measured, and the results
revealed that many compounds exhibited high inhibitions.

Conclusion:
The antiproliferative activity of the synthesized compounds was studied on six selected cancer cell
lines. The nature of the heterocyclic ring and the variations of substituted groups showed a high effect through
the inhibitions of the tested compound.
Unveiling Current Advances in Bioelectronic Medicine: A Comprehensive Review
Authors: Deshkar Sanjeevani S. , Ovhal Kalyani G. , Kothapalli Lata P. , Shirolkar Satish V. , Pagar Roshani R. 
Published: April 2024

Background:
Bioelectronic medicine is an emerging therapy that makes use of neural signals and the nervous system to help in the treatment of injuries and diseases. The nervous sys-tem consists of disciplined circuits that involve the communication of every major organ of the human body as all the organs of the human body are regulated and controlled by neural circuits.

Objective:
The objective of this review is to overview the current implementations of bioelectron-ic medicines within the human body. The main target is to heal the body without administering a wide array of exogenous drugs. On understanding these neural circuits at a molecular level, it could be possible to understand, manipulate, and modify the body’s functioning with the help of electrical impulses to modify neural impulses.

Methods:
The literature related to bioelectronic medicines, and their applications was collected through different websites, academic research portals, and databases, sorted, and presented in this review.

Results:
Bioelectronic medicine is emerging as a cutting-edge area in healthcare, demonstrating its potential to transform the diagnosis and management of inflammation and related conditions. With established efficacy in a variety of disorders and marketed available treatments, it highlights an enormous shift toward individualized and specialized therapeutic approaches, providing prom-ise for improved outcomes and a higher quality of life for patients worldwide.

Conclusion:
The study focuses on potential advances in bioelectronic medicine for alleviating in-flammation and inflammatory disorders such as rheumatoid arthritis, diabetes, and spinal cord in-jury. Bioelectronic treatments provide innovative therapeutic options with the potential for con-siderable clinical effects.
Digital Food Frequency Questionnaire Assessing Adherence to the Norwegian Food–Based Dietary Guidelines and Other National Lifestyle Recommendations: Instrument Validation Study
Authors: Henriksen Hege Berg , Knudsen Markus Dines , Hjartåker Anette , Blomhoff Rune , Carlsen Monica Hauger 
Published: April 2024

Background
Valid assessment tools are needed when investigating adherence to national dietary and lifestyle guidelines.

Objective
The relative validity of the new digital food frequency questionnaire, the DIGIKOST-FFQ, against 7-day weighed food records and activity sensors was investigated.

Methods
In total, 77 participants were included in the validation study and completed the DIGIKOST-FFQ and the weighed food record, and of these, 56 (73%) also used the activity sensors. The DIGIKOST-FFQ estimates the intake of foods according to the Norwegian food–based dietary guidelines (FBDGs) in addition to lifestyle factors.

Results
At the group level, the DIGIKOST-FFQ showed good validity in estimating intakes according to the Norwegian FBDG. The median differences were small and well below portion sizes for all foods except “water” (median difference 230 g/day). The DIGIKOST-FFQ was able to rank individual intakes for all foods (r=0.2-0.7). However, ranking estimates of vegetable intakes should be interpreted with caution. Between 69% and 88% of the participants were classified into the same or adjacent quartile for foods and between 71% and 82% for different activity intensities. The Bland-Altman plots showed acceptable agreements between DIGIKOST-FFQ and the reference methods. The absolute amount of time in “moderate to vigorous intensity” was underestimated with the DIGIKOST-FFQ. However, estimated time in “moderate to vigorous intensity,” “vigorous intensity,” and “sedentary time” showed acceptable correlations and good agreement between the methods. The DIGIKOST-FFQ was able to identify adherence to the Norwegian FBDG and physical activity recommendations.

Conclusions
The DIGIKOST-FFQ gave valid estimates of dietary intakes and was able to identify individuals with different degrees of adherence to the Norwegian FBDG and physical activity recommendations. Moderate physical activity was underreported, water was overreported, and vegetables showed poor correlation, which are important to consider when interpreting the data. Good agreement was observed between the methods in estimating dietary intakes and time in “moderate to vigorous physical activity,” “sedentary time,” and “sleep.”