bluuebunny/crossref_metadata_2025_split huggingface dataset:
A clinical study of CNV-seq techniques in 103 cases of spontaneous abortion
Authors: xie meihua , Zhou Hongxia , Zhuang Jianlong , Chen Jingyi , Li Hening , Gong Yanfei , Li Bojie , Xie※ Liyun , Pi※ Airong 
Published: May 2024

Abstract

Objective: To detect the relationship between spontaneous abortion and chromosomal abnormalities by genome copy number variation sequencing (CNV-seq), and explore this relationship’s application to the genetic diagnosis of spontaneous abortion.
Methods: From June, 2020 to August, 2022, 103 different embryonic tissue samples from patients who suffered spontaneous abortion in Yueyang Central Hospital were detected by CNV-seq, and the results were analyzed and interpreted after excluding maternal cell contamination.
Results: 97 of the 103 cases were detected successfully. A total of 49 cases (50.5%) of abnormal karyotypes were found, including 33 cases of abnormal chromosome number, 12 cases of structural abnormalities (including 14 pathogenic CNVs and 3 variants of uncertain significance,), 3 cases of triploid, and 1 cases of absence of heterozygosity (AOH). The detection rate of chromosomal abnormalities in abortion tissues in the ≥ 35 years old group was higher than that in the <35 years old group (58.8% vs. 48. 7%).
Conclusion: Fetal chromosomal abnormalities are an important cause of abortion, CNV-seq technology can efficiently detect chromosomal number abnormalities, large fragment deletions/duplications and pathogenic genome copy number variants, which greatly improves the detection rate of chromosomal abnormalities and provides a valuable genetic risk assessment to couples trying to reproduce again.
Causal association between helicobacter Pylori infection and Sjogren’s syndrome: a bi-directional Mendelian randomization analysis
Authors: Cui Dinglu , An Rongxian , Li Lei , Jiang Long , Jiang Chunshan , Jin Jingchun 
Published: May 2024

Abstract

Background: Based on the results of existing observational studies, it can be found that there is a potential link between Helicobacter pylori(H.pylori) infection and Sjogren’s syndrome(SS), but the causal relationshipbetween them remains unknown. This study applied Mendelian randomization (MR) to evaluate the causal relationship between H.pylori infection and SS.
Method: In this study, genome-wide association studies (GWAS) summary statistics on H.pylori infection [sample size=8735 (EBI)] and SS [sample size=368,028 (cases=2495, controls=365533) (FinnGen)] were used. we used bi-directional MR to evaluate the association between H.pylori infection and SS for identifying causation. The major analysis method of MR was performed using inverse-variance weighted(IVW), supplemented by MR-Egger and the weighted median approaches. In addition, the stability and reliability of the results were tested using the retention method, heterogeneity test, and horizontal gene pleiotropy test.
Results: The evidence of the impact of H.pylori infection on SS risk was found in IVW results[odds ratio (OR)=1.6705; 95% confdence interval (CI)=1.0966 to 2.5446; P=0.0168]. Similarly, the evidence of the impact of SS on H.pylori infection risk has also been found(OR=1.0158; 95%CI=1.0033 to 1.0285; P=0.0128).
Conclusion: The results of MR analysis support that H.pylori infection was causally associated with SS, and SS can lead to a higher risk of H.pylori infection.We want to provide novel approaches for continued H.pylori and SS-related research and therapy by evaluating the genetic relationship between H.pylori infection and SS.
Mechanical strength of the rotator cuff and cable interface: A complete histological and biomechanical study
Authors: Fondin Maxime , Miroir Mathieu , Guillin Raphaël , Landreau Julien , Ghukasyan Gevorg , Fautrel Alain , Ropars Mickaël , Morandi Xavier , Timoh Krystel Nyangoh , Cam Jean-Benoît Le 
Published: May 2024

Abstract

Purpose This study sought to evaluate the biomechanical properties of the interface between the rotator cuff and the semicircular humeral ligament or rotator cable (RCa) using histological and biomechanical techniques.Methods Out of 13 eligible cadaver specimens, 5 cadaver shoulders with an intact rotator cuff were included, 8 were excluded due to an injured rotator cuff. The histological study enables us to describe the capsule-tendon interface between the infraspinatus tendon (IST) or supraspinatus tendon (SST) and RCa, and to detect loose connective tissue layers to determine their precise location and measure their length along the interface. The biomechanical study sought to characterize and compare the mechanical strength of the IST-RCa versus SST-RCa interfaces.Results The average thickness of the RCa was 1.44 +/- 0.20 mm. The histological study revealed a loose connective tissue layer at the IST-RCa interface, a finding not observed at the SST-RCa interface. The biomechanical study showed that the rigidity of the SST-RCa interface (72.10− 2 N/mm) was 4.5 times higher than for the IST-RCa interface (16.10− 2 N/mm) and the average maximum forces reached were 19 N and 10.6 N for the SST-RCa and IST- RCa interfaces, respectively.Conclusion The IST-RCa interface consists of a loose connective tissue layer contrary to the SST-RCa interface. In parallel, two different groups in terms of the mechanical response were identified: the IST-RCa interface group had less rigidity and ruptured more quickly than the SST-RCa interface, therefore emerging as the most vulnerable interface and explaining a potential extension of rotator cuff tears.
Effect of Maternal Migration on Under-five Mortality in the Navrongo Hdss Area
Authors: Wak George , Oladokun Samuel , Abubakari Sulemana , Komesuor Joyce , Ansah Patrick , Kwankye Stephen 
Published: May 2024

Abstract

Introduction:
Mortality under five years is an important indicator and a significant index for assessing the health and general wellbeing of a country. Even though global efforts to reduce under-five mortality have yielded some positive results, the rates are still high in most low- and middle-income countries. There is general consensus that migration and its associated remittances alleviate poverty at the rural places of origin. This tends to improve household living standards and leads to improvement in child health and survival. This paper seeks to investigate the impact of maternal migration on under-five mortality in two districts in the Upper East Region of Ghana.
Methods
This study used data from the Health and Demographic Surveillance System (HDSS) of the Navrongo Health Research Centre (NHRC) in Ghana. All children (20,990) born in the study area between 2000 and 2014 were included in the analysis. The outcome variable in the analysis was the survival status of the children (dead or alive). The main independent variable is migration status of the mothers (migrants and non-migrants). Proportional Hazard Model, with a Weibull distribution, was used to examine the effect of the independent variables on the survival outcomes of the children.
Results
The results showed that children of migrant mothers are 49% less likely to die compared with children of non-migrant mothers [aOR = 0.513; (CI = 0.451-0585)]. In terms of migration duration before return, survival benefit was highest for children whose mothers had been away for one year and more. Other factors that were found to impact on under-five mortality include maternal education, maternal age, place of delivery, birth order of child, multiple births and the presence of grandmother.
Conclusion
The study has established that maternal migration, irrespective duration, contribute to child survival. Specifically, children of migrant mothers have a better survival chance than children of non-migrant mothers. To improve child survival in these poor rural settings, we recommend the promotion of conducive migration opportunities to enable women to earn some income to support their households in terms of childcare and survival.
Outcomes and prognosis of postpartum hemorrhage according to management protocol: A 11-year retrospective study from two referral centers
Authors: Jung Ye Won , Kim Jin , Shin Won Kyo , Song Soo Youn , Choi Jae Sung , Hyun Suk Hwan , Min Jin Hong , In Yong Nam , Jung Sang Min , Oh Se Kwang , Yoo Heon Jong 
Published: May 2024

Abstract

Background
No standard treatment guidelines have been established for postpartum hemorrhage (PPH). We aimed to assess the differences in outcomes between patients with PPH who underwent surgical and non-surgical treatments.
Methods
This retrospective study included 230 patients diagnosed with PPH at two referral hospitals between August 2013 and October 2023. The patients were divided into non-surgical intervention (group 1, n = 159), surgical intervention (group 2, n = 45), and surgical intervention after failure of non-surgical intervention (group 3, n = 26) groups.
Results
In each group, the initial heart rate and body temperature were significantly different (92.2 ± 20.7, 112.6 ± 30.4, and 102.1 ± 23.3 beats per minute, p < 0.001 and 37.3 ± 0.8°, 36.9 ± 0.9°, and 37.2 ± 0.9°, p = 0.016, in groups 1–3, respectively). Among the causes of PPH, uterine atony combined with other causes showed a significant difference among the three groups (32 cases (20.1%), 8 cases (17.8%), and 14 cases (53.8%), p = 0.003 in groups 1–3, respectively). Mean duration of hospitalization and intensive care unit admission rate were significantly different between the groups (4.1 ± 1.7, 8.7 ± 10.3, and 10.4 ± 13.6 days, p < 0.001 and 6 cases (3.8%), 17 cases (37.8%), and 12 cases (46.2%), p < 0.001 in groups 1–3, respectively). Hysterectomy and disseminated intravascular coagulopathy rates were significantly higher in group 3 than in group 2 [(19 cases (73.1%) and 23 cases (51.1%), x2 = 3.291, p = 0.047); 18 cases (69.2%) and 19 cases (42.2%), x2 = 4.817, p = 0.028), respectively].
Conclusions
In patients with PPH and an initial increased heart rate or decreased body temperature and in those with causes combined with uterine atony, immediate surgical intervention improves the prognosis. Postoperative complications increased when surgery was performed after failure of non-surgical interventions.
Atopic diseases in pediatric population: prematurity and small for gestational age
Authors: Su Yi-Yu , Chen Chi-Jen , Chen Mei-Huei , Lin Ching-Chun , Hsieh Wu-Shiun , Chang Hsi , Chen Chung-Ming , Lin Hsiu-Chen , Chen Pau-Chung 
Published: May 2024

Abstract

The aim of our study was to conduct a national-wide longitudinal follow-up investigation to elucidate the association between prematurity, small for gestational age (SGA), and later development of atopic diseases in pediatric population. Research data was obtained from Taiwan’s National Health Insurance Research Database (NHIRD). Our cohort included infants born between 1 January 2004 and 31 December 2019 with exclusion of death during follow-up period and multiple births. Children born prematurely or SGA were identified as study cases and those born term and appropriate for gestational age (AGA) as controls. Data was then analyzed and adjusted for covariates. A total of 1,758,460 infants comprising 914,713 male and 843,747 female were included in the study. Prematurity was associated with atopic rhinitis (AR) (HR, 1.03, male and HR, 1.03, female), asthma (HR, 1.19, male and HR, 1.17, female) and protective against atopic dermatitis (AD) (HR, 0.94, male and HR, 0.95, female) in both male and female AGA groups. SGA were not associated with atopic diseases in term infants. Further investigations are required to clarify the underlying mechanisms and establish the causal relationship of the issue.
The Impact of Marital Status on Hypertension: SEM Analysis of a Chinese General Population Cohort
Authors: Fan Yajiao , He Huijing , Li Haojun , Chen Zhili , Liu Chang , Ma Lei , Xu Zehua , Zhao Jiayi , Wu Yuhan , Liu Zeqi , Shan Guangliang 
Published: May 2024

Abstract

Background
Hypertension, a chronic non-communicable disease, has multifaceted and complex etiologies influenced by various risk factors. This study examines the role of marital status among the factors influencing blood pressure levels and assesses its potential indirect effects through other variables.
Methods
This study involved 1,852 individuals from the Baoding area in the Beijing-Tianjin-Hebei region, China. Participants were categorized by gender and marital status. Descriptive statistical methods and Structural Equation Modeling (SEM) were utilized for comprehensive data analysis in this study.
Results
Women in the marital turmoil group (divorced or widowed) had higher mean systolic blood pressure compared to those in the married group. A significant age difference between the two groups (p=0.024) may introduce bias into the results. Gender-stratified SEM results show that marital status neither directly nor indirectly affects blood pressure in men. In women, marital status indirectly affects blood pressure through two pathways: "Marital Status (standardized path coefficient, β = 0.15, p < 0.001) - Economic Status (β = -0.33, p < 0.001) - Dietary Habits (β = 0.12, p < 0.05) - Blood Pressure" and "Marital Status (β = 0.15, p < 0.001) - Economic Status (β = -0.33, p < 0.001) - Dietary Habits (β = 0.02, p < 0.01) - Obesity (β = 0.24, p < 0.001) - Blood Pressure."
Conclusion
Results confirm a close association between marital status and hypertension in women, with factors like age and economic status potentially influencing this relationship. This underscores the need for further research to explore the intricate connections between marriage and blood pressure. The study advocates for medical institutions and communities to offer psychological health support to those undergoing marital difficulties and stresses the significance of health education in addressing hypertension risk factors.
A novel Inflammation-Nutrition risk score (INRS) and its related nomogram model to predict radiological bronchiectasis in patients after tuberculosis infection in Wuhan, China
Authors: Yu Qi , Yan Jisong , Song Jianping , Yu Fan , Jiang Nanchuan , Zhou Yaya , He Xinliang , Gong Fengyun , Wang Xiaorong 
Published: May 2024

Abstract

Background
Tuberculosis infection (TBI) is an important cause of bronchiectasis (BE). Exploring the risk factors of radiological BE (RBE) may improve the identification of high-risk populations of RBE after TB infection. The present study aimed to develop a novel inflammation-nutrition risk score (INRS) and RBE nomogram model to identify the high-risk population of RBE after TBI.
Methods
The present study recruited 2015 patients after TBI from two centers. Patients after TBI (N = 1825) from the Wuhan Jinyintan Hospital cohort were used to construct the INRS and RBE nomogram model. In addition, an independent external validation set from the Wuhan Union Hospital (N = 190) validated them.
Results
INRS was constructed based on PNI, HALP score, Lg (SII), and CAR. Additionally, Age ≥ 60 years old (OR:1.19, P = 0.030), Smoker (OR:1.71, P = 0.009), COPD (OR:3.13, P < 0.001), RDW.CV ≥ 12.8 (OR:1.09, P = 0.005), ALB < 35.5 (OR:1.04, P = 0.003), and INRS ≥ 1.86 (OR:5.04, P = < 0.001) were independent risk factors of RBE in patients after TBI. The RBE nomogram model to identify the high-risk RBE population was successfully established and showed a strong discriminative ability, accuracy, and clinical utility in development, internal validation, and external validation sets.
Conclusion
In patients after TBI, INRS may be a useful predictive biomarker for RBE, and the RBE nomogram model is a convenient and efficient tool for follow-up management for preventing RBE.
RETRACTED: Immunotherapy of breast cancer with folate targeted PLGA nanoparticles photothermal therapy combined with checkpoint CTLA4 blocking
Authors: HU CONG , Ke Qianqian , Jiang Yuancheng , Wu Ziming , Chen Yixin , Wu Ziling , Zhang Qi , Wang Ying , WU MENG 
Published: May 2024

Abstract

The authors have requested that this preprint be removed from Research Square.
Peristaltic contractility: Another factor impacting IPAA function?
Authors: Stem Jonathan , Heise Charles , Harms Bruce , Stalter Lily , Marka Nicholas , Carchman Evie 
Published: May 2024

Abstract

Purpose:
This study aims to investigate the phenomenon of high amplitude pouch contractile waves and their impact on functional results in patients undergoing ileal pouch-anal anastomosis following total proctocolectomy for ulcerative colitis.
Methods:
This is a retrospective review of a colorectal database at a single academic center. Patients underwent pouch manometric evaluation at an early (Study 1, <6mo s/p ileostomy closure) and delayed (Study 2, > 5 months after study 1) timepoint. High amplitude contractions were defined as peaks ≥ 20 mmHg over baseline. Pouch functional measures and quality of life outcomes were correlated with contractile amplitude and frequency.
Results:
33 patients were included in this study. Contractile frequency decreased from Study 1 to Study 2 (0.14 vs 0.07 contractions/min). Peristaltic contractility was absent in 18/33 patients (55%) on their early examination (Study 1) after stoma takedown and 23/33 patients (70%) on Study 2. A significant association was observed between increased pouch contraction and nighttime spotting (p = 0.05) during Study 1. Increased amplitude was associated with increased nighttime spotting (p = 0.03) and an increased number of 24-hour bowel movements (p = 0.038) during Study 1. There were no significant associations with pouch function with Study 2. Contractions had no significant impact on quality-of-life outcomes.
Conclusions:
Nighttime spotting is associated with increased contractile frequency and amplitude early in pouch life following ileostomy takedown. Contractility decreases over time, with no evidence of contractile waves in the majority of patients at greater than one-year post-IPAA. High amplitude contractility is an additional factor contributing to early pouch functional results.
Root Exudates Metabolic Profiling Suggests Distinct Defense Mechanisms Between Resistant and Susceptible Konjac Species against Soft Rot Disease
Authors: Wu Jinping , Zhou Jie , Yang Chaozhu , Kuang Yidi , Qi Chuangdong , Guo Fengling , Zhao Qinghua 
Published: May 2024

Abstract

In order to understand the relationship between the changes of konjac root exudates induced by soft rot disease, the differences of root exudates between resistant cultivars (Amorphophallus bulbifer, HB) and susceptible cultivars (Amorphophallus konjac K.Koch, HK) were analyzed by high-performance liquid chromatography tandem mass Fourier transform (UHPLC-q Exactive HF-X). The results showed that the 364 potential metabolites with significant differential amounts. Further analysis of the differential metabolites showed that there were 7 unique metabolites in HB, and 4 unique metabolites in HK. The fold change is 0.6143 and 0.5606 in HK/HB for 3-Fucosyllactose and buddleoflavonoloside, respectively. This showed that some sugars and flavonoids help improve the resistance of konjac soft rot. The analysis of metabolic pathways using the KEGG database revealed that the concentration of jasmonic acid (JA) in HB was significantly higher than in HK. JA metabolism was involved in regulating konjac soft rot disease resistance.
Barriers and facilitators in the acquisition of diabetes knowledge among tertiary-care nurses in central and southern Malawi: An exploratory-descriptive qualitative study
Authors: Nyalapa McDonald William , Gombachika Belinda Thandizo , Gundo Rodwell , Chepuka Lignet 
Published: May 2024

Abstract

Background
Numerous studies report low diabetes knowledge among nurses in sub-Saharan Africa. However, little is known about the factors that hinder and promote their acquisition of diabetes knowledge. Understanding these factors is a pivotal step towards ensuring that nurses are knowledgeable and competent in the provision of care and education for patients with diabetes.
Methods
The study employed an exploratory-descriptive qualitative (EDQ) approach. Participants were identified from two tertiary hospitals. Twenty-six participants were purposively selected from these facilities. Data were collected through focus group discussions and analysis was done using Braun and Clarke’s approach to thematic analysis.
Results
The following themes relating to barriers and facilitators in the nurses’ acquisition of diabetes knowledge emerged from the study: (a) individual factors; (b) organizational factors; and (c) innovative suggestions for improving the nurses’ diabetes knowledge.
Conclusions
The study brings to light various challenges and opportunities in the acquisition of diabetes knowledge among tertiary-care nurses in Malawi. Addressing the barriers to the acquisition of diabetes knowledge among nurses using cost-effective measures is a crucial step towards ensuring that nurses are knowledgeable and competent in the provision of care for patients with diabetes in low-resource countries.
Forecasting step-like landslide displacement through diverse monitoring frequencies
Authors: Guo Fei , Xu zhizhen , Hu Jilei , Dou Jie , Li Xiaowei , Yi Qinglin 
Published: May 2024

Abstract

The accuracy of displacement prediction plays a pivotal role in landslide prevention and control efforts. However, many existing studies have overlooked the influence of surface monitoring frequency on displacement prediction accuracy. In this study, we investigate the impact of different monitoring frequencies on displacement prediction accuracy using the Baijiabao landslide in the Three Gorges Reservoir Area (TGRA) as a case study. We gathered landslide surface automatic monitoring data at varying monitoring frequencies, including daily, seven days, nine days, eleven days, thirteen days, fifteen days, twenty-one days, and thirty days. To analyze the data, we employed the Ensemble Empirical Mode Decomposition (EEMD) algorithm to decompose accumulated displacements into periodic term displacements and trend term displacements at each monitoring frequency. Subsequently, we predicted the trend term displacement using polynomial fitting, while the periodic term displacement was forecasted using two neural network models: the Long Short-Term Memory model (LSTM) and the Gated Recurrent Unit model (GRU). These predictions were then combined to obtain cumulative displacement predictions, allowing us to compare the prediction accuracies across different monitoring frequencies. Our findings indicate that the proposed prediction models exhibit robust performance in forecasting landslide displacement. Notably, the models' prediction accuracies are highest at moderate monitoring frequencies, surpassing those of daily and monthly monitoring frequencies. As monitoring frequency increases, the daily mean average error (MAE) experiences a rapid decline before stabilizing. Similar research results were also observed when analyzing the Bazimen landslide, corroborating that displacement prediction at moderate monitoring frequencies (approximately 7 to 15 days) yields superior accuracy compared to daily and monthly monitoring frequencies.