bluuebunny/crossref_metadata_2025_split huggingface dataset:
Platelet P‐selectin reflects a state of cutaneous inflammation: possible application to monitor treatment efficacy in psoriasis
Authors: Garbaraviciene Jurate , Diehl Sandra , Varwig Domenica , Bylaite Matilda , Ackermann Hanns , Ludwig Ralf J. , Boehncke Wolf‐Henning 
Published: May 2010

Please cite this paper as: Platelet P‐selectin reflects a state of cutaneous inflammation: possible application to monitor treatment efficacy in psoriasis. Experimental Dermatology 2010; 19: 736–741.Abstract:  Haemostasis‐maintaining platelets are also recognized as important modulators in the regulation of immune response. Activated platelets expressing P‐selectin (CD62P) are involved in the extravasation of leucocytes. This study evaluated platelet P‐selectin expression as a biomarker for cutaneous inflammation. P‐selectin expression was assessed by flow cytometry in 147 successive patients suffering from an inflammatory or infectious skin condition at the day of admission for in‐patient treatment as well as a day prior to demission. Forty‐one patients admitted for allergy testing served as controls. A commercially available ELISA was used in 17 patients to determine soluble P‐selectin in the plasma. In patients with psoriasis, the Psoriasis Area and Severity Index (PASI) was documented as a measure for disease severity. We observed a significant increase in platelet P‐selectin expression in patients with inflammatory or infectious disorders, when compared to the control group (3,01% vs. 1,46%; P < 0.000001). Successful treatment resulted in a significant decrease in P‐selectin expression to the level of the control group. In the case of psoriasis (n = 47), we found highly significant correlation between P‐selectin and PASI (r = 0.51; P < 0.000001), as well as between the change in the PASI and the change in P‐selectin expression (r = 0.4; P = 0.006). Platelet P‐selectin expression as determined by flow cytometry correlated well with the results of soluble P‐selectin, determined by ELISA (r = 0.63; P < 0.01). Thus, platelet P‐selectin expression may be used as an efficacy biomarker to monitor treatment success in psoriasis. As platelet P‐selectin correlates with soluble P‐selectin in patient plasma, which can be measured by ELISA, the latter is feasible also for routine use.
Tilted Implants for the Rehabilitation of Edentulous Jaws: A Systematic Review
Authors: Del Fabbro Massimo , Bellini Chiara M. , Romeo Davide , Francetti Luca 
Published: May 2010

ABSTRACTPurpose: The aim of this review was to evaluate the survival rate of upright and tilted implants supporting fixed prosthetic reconstructions for the immediate rehabilitation of partially and fully edentulous jaws, after at least 1 year of function.Materials and Methods: An electronic search of databases plus a hand search on the most relevant journals up to December 2009 was performed. The articles were selected using specific inclusion criteria, independent of the study design.Results: The literature search yielded 347 articles. A first screening based on the title and abstract identified 25 eligible studies. After full‐text review of these studies, 10 articles were selected for analysis. Seven were prospective single‐cohort studies and three had a retrospective design. A total of 462 patients have been rehabilitated with 470 immediately loaded prostheses (257 in the maxilla, 213 in the mandible), supported by a total of 1,992 implants (1,026 upright and 966 tilted). Twenty‐five implants (1.25%) failed in 20 patients within the first year. All failures except one occurred in the maxilla. No significant difference in failure rate was found between tilted and upright implants, nor between maxillary and mandibular implants. No prosthesis failure was reported. Limited peri‐implant bone loss was reported with no difference between upright and tilted implants. Full patients' satisfaction for function, phonetics, and esthetics was reported in three studies, based on questionnaires.Conclusions: The use of tilted implants to support immediately loaded fixed prostheses for the rehabilitation of edentulous jaws can be considered a predictable technique, with an excellent prognosis in the short‐medium term. However, randomized long‐term trials are needed to determine the efficacy of this surgical approach.
Effects of starch and protein sources on starch disappearance in the gastrointestinal tract of Hanwoo (Korean native) steers
Authors: KIM Kyoung Hoon , JIN Guang‐Lin , OH Young‐Kyoon , SONG Man‐Kang 
Published: May 2010

ABSTRACTHanwoo (Korean native) steers (274.8 ± 4.6 kg) with ruminal and duodenal cannulae were used in a 4 × 4 Latin square design experiment to examine the effects of dietary treatments on starch disappearance in the gastrointestinal tract. Dietary treatments consisted of concentrate that were based on ground corn with soybean meal (C‐SBM), ground corn with corn gluten meal (C‐CGM), ground barley with soybean meal (B‐SBM) and ground barley with corn gluten meal (B‐CGM). Although the intakes of starch and protein for steers fed experimental diets were different, it did not change ruminal pH and total volatile fatty acid concentrations. Average duodenal CP flow and quantity of CP apparently digested post‐ruminally was higher (P = 0.001) for CGM‐based diets than SBM‐based diets. There were increases in quantity (P < 0.001) and percentage (P < 0.001) of corn starch digested post‐ruminally compared to barley starch. Synchronized diets showed higher percentages (P = 0.03) of starch apparently digested post‐ruminally than asynchronization. Hanwoo steers fed a corn‐based diet with a large quantity of starch reaching the duodenum and fed C‐CGM supplying great amounts of protein to the small intestine may have contributed to increased post‐ruminal starch digestion.
Age‐related changes in semen quality characteristics and expectations of reproductive longevity in Duroc boars
Authors: HUANG Yu Hung , LO Ling Ling , LIU Shyh Hwa , YANG Tien Shuh 
Published: May 2010

ABSTRACTQuadratic fitting was used to regress semen characteristics of 1441 samples consisting of 12‐month collection from 58 Duroc boars against animal age varied from 10 to 80 months. Data was divided into two groups of cool (14.0–22.7°C, RH 81.5%) and hot season (22.9–29.9°C, RH 86.6%), to test effects of age, season and their interactions. Results revealed that young boars of around 1 year old could endure the hot season. The endurance gradually diminished as animals grew. In the hot season animals exhibited peak performance at age around 33 month and it remained for 1 month, while cool‐season kept boars could last for 48 months from 16 months old onward. The reproductive longevity should be 51 month in a subtropical environment and it may extend to 70 month if heat stress can be avoided. The estimated total sperm contribution of a Duroc boar would be 1.8 times more when kept below 22°C than in a natural subtropical environment. It is concluded that to maintain Duroc boars as semen donor to at least 4 years of age is feasible in a subtropical environment and boar longevity could reach 6 years old if well kept in a temperate region.
Porcine CPEB1 is involved in Cyclin B translation and meiotic resumption in porcine oocytes
Authors: NISHIMURA Yukio , KANO Kiyoshi , NAITO Kunihiko 
Published: May 2010

ABSTRACTOvarian immature oocytes accumulate many dormant maternal mRNAs, which have short poly(A) tails. Cytoplasmic‐polyadenylation‐element binding protein (CPEB) has been reported to play key roles for the elongation of the tails and the translation of these mRNAs in Xenopus oocytes. However, the functions of CPEB in meiotic resumption have not yet been established in mammalian oocytes. The present study examined the roles of porcine CPEB in Cyclin B syntheses and meiotic resumption of porcine oocytes. Porcine CPEB1 (pCPEB1) cDNA was cloned from total RNA of immature oocytes by RT‐PCR. The overexpression of pCPEB1 by mRNA injection into immature oocytes increased Cyclin B expression and the rate of meiotic resumption. Conversely, the inhibition of endogenous CPEB by expression of a dominant‐negative mutant pCPEB1 (AA‐CPEB), which replaced the expected phosphorylation sites with alanines, had the effect of inhibiting Cyclin B synthesis, ribosomal S6 kinase phosphorylation (an indicator of Mos activity), and meiotic resumption. The inhibition of porcine Aurora A by an injection of antisense RNA enhanced the inhibitory effects of AA‐CPEB. These results suggest the involvement of mammalian CPEB1 in Cyclin B syntheses and meiotic resumption in mammalian oocytes. In addition, the phosphorylation sites of pCPEB1 were identified and are suggested to be phosphorylated by porcine Aurora A.
Adverse Events in Therapeutic Apheresis: A Single Center Survey of Various Therapies
Authors: Ishihara Takeshi , Inoue Satoko , Takagi Yukiko , Shimomura Taro , Sagami Yoshiaki , Katayama Sayomi , Eguchi Yoko , Watase Kenji , Miyamoto Masahiko , Minakata Tamotsu 
Published: May 2010

AbstractThe aim of the study was to review the adverse events associated with various treatment modalities performed in a single apheresis facility. A total of 854 sessions with 10 types of apheresis therapies were performed and 154 (18.0%) adverse events were observed over a four‐year period. Of the adverse events, 77 were related to operational problems and another 77 were complications associated with treatment. A transmembranous pressure abnormality constituted more than 80% of the operational problems. Nausea was the most frequent complication, accounting for 19 of the 77 treatment‐related events. A total of 26 (16.9%) adverse events occurred in the early stage of the sessions, 40 (26.0%) in the middle stage, and 88 (57.1%) in the late stage. The information in this study can be used to improve the safety and efficacy of apheresis therapy.
Hydrogeologic Framework of Fractured Sedimentary Rock, Newark Basin, New Jersey
Authors: Lacombe Pierre J. , Burton William C. 
Published: May 2010

The hydrogeologic framework of fractured sedimentary bedrock at the former Naval Air Warfare Center (NAWC),1 Trenton, New Jersey, a trichloroethylene (TCE)‐contaminated site in the Newark Basin, is developed using an understanding of the geologic history of the strata, gamma‐ray logs, and rock cores. NAWC is the newest field research site established as part of the U.S. Geological Survey Toxic Substances Hydrology Program, Department of Defense (DoD) Strategic Environmental Research and Development Program, and DoD Environmental Security Technology Certification Program to investigate contaminant remediation in fractured rock.Sedimentary bedrock at the NAWC research site comprises the Skunk Hollow, Byram, and Ewing Creek Members of the Lockatong Formation and Raven Rock Member of the Stockton Formation. Muds of the Lockatong Formation that were deposited in Van Houten cycles during the Triassic have lithified to form the bedrock that is typical of much of the Newark Basin. Four lithotypes formed from the sediments include black, carbon‐rich laminated mudstone, dark‐gray laminated mudstone, light‐gray massive mudstone, and red massive mudstone. Diagenesis, tectonic compression, off‐loading, and weathering have altered the rocks to give some strata greater hydraulic conductivity than other strata. Each stratum in the Lockatong Formation is 0.3 to 8 m thick, strikes N65°E, and dips 25° to 70°NW. The black, carbon‐rich laminated mudstone tends to fracture easily, has a relatively high hydraulic conductivity and is associated with high natural gamma‐ray count rates. The dark‐gray laminated mudstone is less fractured and has a lower hydraulic conductivity than the black carbon‐rich laminated mudstone. The light‐gray and the red massive mudstones are highly indurated and tend to have the least fractures and a low hydraulic conductivity.The differences in gamma‐ray count rates for different mudstones allow gamma‐ray logs to be used to correlate and delineate the lithostratigraphy from multiple wells. Gamma‐ray logs and rock cores were correlated to develop a 13‐layer gamma‐ray stratigraphy and 41‐layer lithostratigraphy throughout the fractured sedimentary rock research site.Detailed hydrogeologic framework shows that black carbon‐rich laminated mudstones are the most hydraulically conductive. Water‐quality and aquifer‐test data indicate that groundwater flow is greatest and TCE contamination is highest in the black, carbon‐ and clay‐rich laminated mudstones. Large‐scale groundwater flow at the NAWC research site can be modeled as highly anisotropic with the highest component of permeability occurring along bedding planes.
Expression of the inulinase gene from the marine‐derived Pichia guilliermondii in Saccharomyces sp. W0 and ethanol production from inulin
Authors: Zhang Tong , Chi Zhe , Chi Zhenming , Parrou Jean‐Luc , Gong Fang 
Published: May 2010

SummaryIt has been confirmed that Saccharomyces sp. W0 can produce high concentration of ethanol. In this study, the INU1 gene cloned from the marine‐derived Pichia guilliermondii was transformed into uracil mutant of Saccharomyces sp. W0. The positive transformant Inu‐66 obtained could produce 34.2 U ml−1 of extracellular inulinase within 72 h of cultivation. It was found that 15.2 U of inulinase activity per one gram of inulin was suitable for inulin hydrolysis and ethanol production by the transformant Inu‐66. During the small‐scale fermentation, 13.7 ml of ethanol in 100 ml of medium was produced and 99.1% of the added inulin was utilized by the transformant. During the 2 l fermentation, 14.9% (v/v) of ethanol was produced from inulin and 99.5% of the added inulin was converted into ethanol, CO2 and cell mass.
Microbial diagnostic microarray for food‐ and water‐borne pathogens
Authors: Kostić Tanja , Stessl Beatrix , Wagner Martin , Sessitsch Angela , Bodrossy Levente 
Published: May 2010

SummaryA microbial diagnostic microarray for the detection of the most relevant bacterial food‐ and water‐borne pathogens and indicator organisms was developed and thoroughly validated. The microarray platform based on sequence‐specific end labelling of oligonucleotides and the pyhylogenetically robust gyrB marker gene allowed a highly specific (resolution on genus/species level) and sensitive (0.1% relative and 104 cfu absolute detection sensitivity) detection of the target pathogens. Validation was performed using a set of reference strains and a set of spiked environmental samples. Reliability of the obtained data was additionally verified by independent analysis of the samples via fluorescence in situ hybridization (FISH) and conventional microbiological reference methods. The applicability of this diagnostic system for food analysis was demonstrated through extensive validation using artificially and naturally contaminated spiked food samples. The microarray‐based pathogen detection was compared with the corresponding microbiological reference methods (performed according to the ISO norm). Microarray results revealed high consistency with the reference microbiological data.
Monte Carlo integration over stepping stone models for spatial genetic inference using approximate Bayesian computation
Authors: BAIRD STUART J. E. , SANTOS FILIPE 
Published: May 2010

AbstractApproximate Bayesian computation (ABC) substitutes simulation for analytic models in Bayesian inference. Simulating evolutionary scenarios under Kimura’s stepping stone model (KSS) might therefore allow inference over spatial genetic process where analytical results are difficult to obtain. ABC first creates a reference set of simulations and would proceed by comparing summary statistics over KSS simulations to summary statistics from localities sampled in the field, but: comparison of which localities and stepping stones? Identical stepping stones can be arranged so two localities fall in the same stepping stone, nearest or diagonal neighbours, or without contact. None is intrinsically correct, yet some choice must be made and this affects inference. We explore a Bayesian strategy for mapping field observations onto discrete stepping stones. We make Sundial, for projecting field data onto the plane, available. We generalize KSS over regular tilings of the plane. We show Bayesian averaging over the mapping between a continuous field area and discrete stepping stones improves the fit between KSS and isolation by distance expectations. We make Tiler Durden available for carrying out this Bayesian averaging. We describe a novel parameterization of KSS based on Wright’s neighbourhood size, placing an upper bound on the geographic area represented by a stepping stone and make it available as m Vector. We generalize spatial coalescence recursions to continuous and discrete space cases and use these to numerically solve for KSS coalescence previously examined only using simulation. We thus provide applied and analytical resources for comparison of stepping stone simulations with field observations.
Strong HIV-Specific CD4
+
and CD8
+
T-Lymphocyte Proliferative Responses in Healthy Individuals Immunized with an HIV-1 DNA Vaccine and Boosted with Recombinant Modified Vaccinia Virus Ankara Expressing HIV-1 Genes
Authors: Aboud Said , Nilsson Charlotta , Karlén Katarina , Marovich Mary , Wahren Britta , Sandström Eric , Gaines Hans , Biberfeld Gunnel , Godoy-Ramirez Karina 
Published: May 2010

ABSTRACT

We investigated HIV-1 vaccine-induced lymphoproliferative responses in healthy volunteers immunized intradermally or intramuscularly (with or without adjuvant granulocyte-macrophage colony-stimulating factor [GM-CSF] protein) with DNA expressing HIV-1
gag
,
env
,
rev
, and
rt
at months 0, 1, and 3 using a Biojector and boosted at 9 months with modified vaccinia virus Ankara (MVA) expressing heterologous HIV-1
gag
,
env
, and
pol
(HIV-MVA). Lymphoproliferative responses to aldrithiol-2 (AT-2)-inactivated-HIV-1 antigen were tested by a [
3
H]thymidine uptake assay and a flow-cytometric assay of specific cell-mediated immune response in activated whole blood (FASCIA-WB) 2 weeks after the HIV-MVA boost (
n
= 38). A FASCIA using peripheral blood mononuclear cells (FASCIA-PBMC) was also employed (
n
= 14). Thirty-five of 38 (92%) vaccinees were reactive by the [
3
H]thymidine uptake assay. Thirty-two of 38 (84%) vaccinees were reactive by the CD4
+
T-cell FASCIA-WB, and 7 of 38 (18%) also exhibited CD8
+
T-cell responses. There was strong correlation between the proliferative responses measured by the [
3
H]thymidine uptake assay and CD4
+
T-cell FASCIA-WB (
r
= 0.68;
P
< 0.01). Fourteen vaccinees were analyzed using all three assays. Ten of 14 (71%) and 11/14 (79%) demonstrated CD4
+
T-cell responses in FASCIA-WB and FASCIA-PBMC, respectively. CD8
+
T-cell reactivity was observed in 3/14 (21%) and 7/14 (50%) using the FASCIA-WB and FASCIA-PBMC, respectively. All 14 were reactive by the [
3
H]thymidine uptake assay. The overall HIV-specific T-cell proliferative response in the vaccinees employing any of the assays was 100% (38/38). A standardized FASCIA-PBMC, which allows simultaneous phenotyping, may be an option to the [
3
H]thymidine uptake assay for assessment of vaccine-induced T-cell proliferation, especially in isotope-restricted settings.
Dendritic Cells in Uninfected Infants Born to Hepatitis B Virus-Positive Mothers
Authors: Koumbi Lemonica J. , Papadopoulos Nikolaos G. , Anastassiadou Vassiliki , Machaira Maria , Kafetzis Dimitris A. , Papaevangelou Vassiliki 
Published: May 2010

ABSTRACT
Plasmacytoid dendritic cells (pDCs) play a central role in antiviral immunity, detecting viruses via Toll-like receptors (TLR) and producing in response vast amounts of type I interferons (IFNs). Hepatitis B virus (HBV) causes chronic infection after vertical transmission. This study investigated whether an HBV-infected maternal environment might influence DC numbers and pDC function in uninfected infants. Blood was collected from inactive HBsAg carrier and control mothers and their infants at birth and 1 and 6 months of age. HBV DNA was measured in maternal and neonatal perinatal sera using real-time PCR. The circulating frequencies of myeloid DCs (mDCs) and pDCs were determined in the babies by flow cytometry. Peripheral blood mononuclear cells (PBMCs) and cord blood pDCs were stimulated with resiquimod, and alpha interferon (IFN-α) production and the pDC phenotype were assessed. The effect of the common-cold virus, rhinovirus (RV), on resiquimod stimulation was also determined. HBV DNA was detected in 62.3% of the mothers and 41% of their infants. DC numbers and pDC functions were similar between subjects and controls and were not correlated with maternal or neonatal viremia. RV infection did not induce pDC maturation until the age of 6 months, and it reduced TLR7-dependent resiquimod-induced IFN-α production similarly in both groups. Although the DC system is immature at birth, DCs of uninfected neonates of HBV-positive mothers are competent to initiate and maintain T-cell responses. RV is a weak inducer of IFN-α production until the age of 6 months and inhibits IFN-α responses triggered by the TLR7 pathway.
Functional T-Cell Deficiency in Adolescents Who Experience Serogroup C Meningococcal Disease despite Receiving the Meningococcal Serogroup C Conjugate Vaccine
Authors: Foster Rachel A. , Carlring Jennifer , Lees Andrew , Borrow Ray , Ramsay Mary , Kacsmarski Ed , Miller Elizabeth , McKendrick Michael W. , Heath Andrew W. , Read Robert C. 
Published: May 2010

ABSTRACTSome individuals have experienced meningococcal disease despite receiving the meningococcal serogroup C conjugate (MCC) vaccine in adolescence. We sought to determine whether this is due to subclinical functional B- or T-cell immunodeficiency. Of 53 vaccine failures identified by enhanced surveillance of England and Wales from 1999 to 2004, 15 received MCC vaccine in adolescence, 9 of whom were recruited 2 to 6 years following convalescence from meningococcal disease. Their peripheral blood mononuclear cells (PBMCs) were incubated with polyclonal activators designed to mimic T-cell-independent B-cell stimulation by bacterial polysaccharides and the T-cell stimulation provided by the protein component of the conjugate vaccine. Subsequent proliferation and activation of T and B lymphocytes were measured, along with T-cell help to B cells. Compared to age-, sex-, geographically, and ethnicity-matched controls, CD4 T-cell proliferation rates in response to both anti-CD3 (T-cell receptor [TCR]) stimulation and anti-CD3 in the presence of B cells activated through anti-IgD conjugated to dextran (α-δ-dex) were lower in PBMCs derived from vaccine failures (P= 0.044 andP= 0.029, respectively). There was reduced CD4 cell activation of the patient cells compared to controls following stimulation by CD3 (P= 0.048). B-cell activation during incubation of PBMCs with the T-cell stimuli, anti-CD3 (P= 0.044), or anti-CD3 plus anti-CD28 (P= 0.018) was relatively impaired in patients. Anti-tetanus toxoid IgG concentrations were lower in the vaccine failure group (P= 0.0385). There was a relative defect of T-cell responsiveness to T-cell-dependent antigen stimulation in MCC vaccine failures, which was manifested in reduced T-cell help to B cells.