bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feasibility assessment of a clean and efficient fire extinguishing system for pottery jar liquor warehouses
Authors: Li Xueming , Wan Wei , Zhao Youkai , Bai Gang , Shi Xunxian , Chen Bing , Zhang Yutao 
Published: June 2024

AbstractClean fire extinguishing systems applicable to the pottery jar liquor warehouse are in demand. In this study, taking 53vol% liquor as the research subject, fire models of various clean fire extinguishing systems comprising water mist, liquid carbon dioxide (LCO2) and liquid nitrogen (LN2) were established using a fire dynamic simulator to determine their fire extinguishing effect. A feasibility assessment of systems was performed under different fire source types, fire source sizes, and ventilation conditions. The fire extinguishing efficiency was analyzed in terms of the fire extinguishing time, oxygen concentration, and space temperature. The results showed that the success rate of the LCO2 and LN2 fire extinguishing systems was 100%, whereas the success rate of the water mist fire extinguishing system was 95%. In terms of reducing the oxygen concentration at the bottom of the space and the temperature in the space, the LCO2 system exhibited the best performance, followed by the LN2 system, and lastly the water mist. Under different ventilation conditions and fire source types, the LCO2 fire extinguishing system was least affected, whereas the effectiveness of the water mist fire extinguishing system reduced under natural ventilation conditions, and the extinguishing efficiency of the LN2 fire extinguishing system was affected by the fire source type. Overall, the LCO2 system presented more advantages in extinguishing fires in pottery jar liquor warehouses and can provide a new idea for the development and application of clean and efficient fire extinguishing systems.
Metataxonomic analysis of endophytic bacteria of blackberry (Rubus ulmifolius Schott) across tissues and environmental conditions
Authors: Roca-Couso Rocío , Flores-Félix José David , Deb Saptarathi , Giagnoni Lucia , Tondello Alessandra , Stevanato Piergiorgio , Squartini Andrea , García-Fraile Paula , Rivas Raúl 
Published: June 2024

Abstract(1) Background: Endophytic bacteria represent an important component of plant wellness. They have been widely studied for their involvement in plant development and enhancement of stress tolerance. In this work, the endophytic communities of roots, stems, and leaves of blackberry (Rubus ulmifolius Schott) were studied in three different niches: natural, riverside, and human-impacted niches. (2) Results: The microbiome composition revealed that Sphingomonadaceae was the most abundant family in all samples, accounting for 9.4–45.8%. In contrast, other families seem to be linked to a specific tissue or niche. Families Microbacteriaceae and Hymenobacteraceae increased their presence in stem and leaf samples, while Burkholderiaceae abundance was important in riverside samples. Alpha and beta diversity analyses showed that root samples were the most diverse, and they gathered together in the same cluster, apart from the rest of the samples. (3) Conclusions: The analysis of the microbiome of R. ulmifolius plants revealed that the composition was essentially the same in different niches; the differences were primarily influenced by plant tissue factors with a core genome dominated by Sphingomonadaceae. Additionally, it was observed that R. ulmifolius can select its own microbiome, and this remains constant in all tissues evaluated regardless the niche of sampling.
Exact analytical soliton solutions of the M-fractional Akbota equation
Authors: Awadalla Muath , Taishiyeva Aigul , Myrzakulov Ratbay , Alahmadi Jihan , Zaagan Abdullah A. , Bekir Ahmet 
Published: June 2024

Abstract
In this paper we explore the new analytical soliton solutions of the truncated M-fractional nonlinear

$$(1+1)$$

(
1
+
1
)

-dimensional Akbota equation by applying the

$$\exp _a$$

exp
a

function technique, Sardar sub-equation and generalized kudryashov techniques. Akbota is an integrable equation which is Heisenberg ferromagnetic type equation and have much importance for the analysis of curve as well as surface geometry, in optics and in magnets. The obtained results are in the form of dark, bright, periodic and other soliton solutions. The gained results are verified as well as represented by two-dimensional, three-dimensional and contour graphs. The gained results are newer than the existing results in the literature due to the use of fractional derivative. The obtained results are very helpful in optical fibers, optics, telecommunications and other fields. Hence, the gained solutions are fruitful in the future study for these models. The used techniques provide the different variety of solutions. At the end, the applied techniques are simple, fruitful and reliable to solve the other models in mathematical physics.
Efficacy and safety of antithrombotic therapy for preventing and treating pediatric thromboembolic disease: a systematic review
Authors: Gao Hongjin , Chen Mingyu , Huang Youqi , Liu Huiting , Lin Yuze , Chen Min 
Published: June 2024

AbstractThis review used traditional and network meta-analyses (NMA) to conduct a comprehensive study of antithrombotic therapies in children with thromboembolic disease. We searched the PubMed, Embase, Cochrane Library, Web of Science and ClinicalTrials.gov databases from their inception to 26 February, 2023. And we finally included 16 randomized controlled trials. In the prevention of thromboembolic events (TEs), the use of anticoagulants had a low risk of TEs (relative risk (RR) 0.73, 95% CI 0.56 to 0.94) and a high risk of minor bleeding (RR 1.43, 95% CI 1.09 to 1.86) compared with no anticoagulants. In the treatment of TEs, direct oral anticoagulants (DOACs) were not inferior to standard anticoagulation in terms of efficacy and safety outcomes. In NMA, rivaroxaban and apixaban showed the lowest risk for TEs and major or clinically relevant nonmajor bleeding. According to the overall assessment of efficacy and safety, dabigatran may be the best choice for children with thromboembolic disease. The results of our study will provide references and suggestions for clinical drug selection.
Enzyme-independent role of EZH2 in regulating cell cycle progression via the SKP2-KIP/CIP pathway
Authors: Colon Tania , Kou Ziyue , Choi Byeong Hyeok , Tran Franklin , Zheng Edwin , Dai Wei 
Published: June 2024

AbstractWhile EZH2 enzymatic activity is well-known, emerging evidence suggests that EZH2 can exert functions in a methyltransferase-independent manner. In this study, we have uncovered a novel mechanism by which EZH2 positively regulates the expression of SKP2, a critical protein involved in cell cycle progression. We demonstrate that depletion of EZH2 significantly reduces SKP2 protein levels in several cell types, while treatment with EPZ-6438, an EZH2 enzymatic inhibitor, has no effect on SKP2 protein levels. Consistently, EZH2 depletion leads to cell cycle arrest, accompanied by elevated expression of CIP/KIP family proteins, including p21, p27, and p57, whereas EPZ-6438 treatment does not modulate their levels. We also provide evidence that EZH2 knockdown, but not enzymatic inhibition, suppresses SKP2 mRNA expression, underscoring the transcriptional regulation of SKP2 by EZH2 in a methyltransferase-independent manner. Supporting this, analysis of the Cancer Genome Atlas database reveals a close association between EZH2 and SKP2 expression in human malignancies. Moreover, EZH2 depletion but not enzymatic inhibition positively regulates the expression of major epithelial-mesenchymal transition (EMT) regulators, such as ZEB1 and SNAIL1, in transformed cells. Our findings shed light on a novel mechanism by which EZH2 exerts regulatory effects on cell proliferation and differentiation through its methyltransferase-independent function, specifically by modulating SKP2 expression.
Applications of magnesium iodide structure via modified-polynomials
Authors: Ghazwani Haleemah , Jamil Muhammad Kamran , Ahmad Ali , Azeem Muhammad , Koam Ali N. A. 
Published: June 2024

Abstract
A relatively recent approach in molecular graph theory for analyzing chemical networks and structures is called a modified polynomial. It emphasizes the characteristics of molecules through the use of a polynomial-based procedure and presents numerical descriptors in algebraic form. The Quantitative Structure-Property Relationship study makes use of Modified Polynomials (M-Polynomials) as a mathematical tool. M-Polynomials used to create connections between a material’s various properties and its structural characteristics. In this study, we calculated several modified polynomials and gave a polynomial description of the magnesium iodide structure. Particularly, we computed first, second and modified Zagreb indices based M-polynomials. Randić index, and inverse Randić indices based M-polynomials are also computed in this work.
Upregulation of sperm-associated antigen 5 expression in endometrial carcinoma was associated with poor prognosis and immune dysregulation, and promoted cell migration and invasion
Authors: Chen Manru , Wang Dan , Xu Yanyu , Yang Chenggang 
Published: June 2024

AbstractSperm-associated antigen 5 (SPAG5) regulates cancer cell invasion and is involved in the progression of many cancers. However, the role of SPAG5 in endometrial carcinoma (EC) is still unknown. The purpose of this study was to explore the role of SPAG5 in EC and its potential molecular mechanism. The UALCAN tool and cBioPortal were used to analyze the expression and alterations of SPAG5 in EC, respectively. OncoLnc was used for survival analysis. We analyzed the effects of SPAG5 on immune cell infiltration and the expression levels of immune checkpoints. We also overexpressed and knocked down SPAG5 in EC cells to explore the effect of SPAG5 regulation on migration, invasion, apoptosis, and the cell cycle of EC cells. We found that SPAG5 was overexpressed and the SPAG5 gene was often mutated in EC. High SPAG5 expression was significantly associated with poor overall survival in patients with EC. SPAG5 also affected the level of immune cell infiltration in the TIME and the expression of immune checkpoints lymphocyte activating 3 (LAG3) and T cell immunoreceptor with Ig and ITIM domains (TIGIT) in patients with EC. It may also be involved in the immunotherapy response in these patients. In vitro experiments showed that SPAG5 promotes cancer cell migration and invasion. In conclusion, this study lays the foundation for further understanding the molecular mechanisms of EC involving SPAG5 and contributes to diagnosing and managing this disease.
miRNA-seq analysis of high glucose induced osteoblasts provides insight into the mechanism underlying diabetic osteoporosis
Authors: Zhang Yang , Li Mengying , Lou Pengqiang , Zhang Minjie , Shou Dan , Tong Peijian 
Published: June 2024

AbstractThe present study aims to explore the etiology of Diabetic osteoporosis (DOP), a chronic complication associated with diabetes mellitus. Specifically, the research seeks to identify potential miRNA biomarkers of DOP and investigated role in regulating osteoblasts. To achieve this, an animal model of DOP was established through the administration of a high-sugar and high-fat diet, and then injection of streptozotocin. Bone microarchitecture and histopathology analysis were analyzed. Rat calvarial osteoblasts (ROBs) were stimulated with high glucose (HG). MiRNA profiles of the stimulated osteoblasts were compared to control osteoblasts using sequencing. Proliferation and mineralization abilities were assessed using MTT assay, alkaline phosphatase, and alizarin red staining. Expression levels of OGN, Runx2, and ALP were determined through qRT-PCR and Western blot. MiRNA-sequencing results revealed increased miRNA-702-5p levels. Luciferase reporter gene was utilized to study the correlation between miR-702-5p and OGN. High glucose impaired cell proliferation and mineralization in vitro by inhibiting OGN, Runx2, and ALP expressions. Interference with miR-702-5p decreased OGN, Runx2, and ALP levels, which were restored by OGN overexpression. Additionally, downregulation of OGN and Runx2 in DOP rat femurs was confirmed. Therefore, the miRNA-702-5p/OGN/Runx2 signaling axis may play a role in DOP, and could be diagnostic biomarker and therapeutic target for not only DOP but also other forms of osteoporosis.
A targeted likelihood estimation comparing cefepime and piperacillin/tazobactam in critically ill patients with community-acquired pneumonia (CAP)
Authors: Serrano-Mayorga Cristian C. , Duque Sara , Ibáñez-Prada Elsa D. , Garcia-Gallo Esteban , Arrieta María P. Rojas , Bastidas Alirio , Rodríguez Alejandro , Martin-Loeches Ignacio , Reyes Luis F. 
Published: June 2024

AbstractCefepime and piperacillin/tazobactam are antimicrobials recommended by IDSA/ATS guidelines for the empirical management of patients admitted to the intensive care unit (ICU) with community-acquired pneumonia (CAP). Concerns have been raised about which should be used in clinical practice. This study aims to compare the effect of cefepime and piperacillin/tazobactam in critically ill CAP patients through a targeted maximum likelihood estimation (TMLE). A total of 2026 ICU-admitted patients with CAP were included. Among them, (47%) presented respiratory failure, and (27%) developed septic shock. A total of (68%) received cefepime and (32%) piperacillin/tazobactam-based treatment. After running the TMLE, we found that cefepime and piperacillin/tazobactam-based treatments have comparable 28-day, hospital, and ICU mortality. Additionally, age, PTT, serum potassium and temperature were associated with preferring cefepime over piperacillin/tazobactam (OR 1.14 95% CI [1.01–1.27], p = 0.03), (OR 1.14 95% CI [1.03–1.26], p = 0.009), (OR 1.1 95% CI [1.01–1.22], p = 0.039) and (OR 1.13 95% CI [1.03–1.24], p = 0.014)]. Our study found a similar mortality rate among ICU-admitted CAP patients treated with cefepime and piperacillin/tazobactam. Clinicians may consider factors such as availability and safety profiles when making treatment decisions.
Heat shock protein 70 kDa (HSP70) is involved in the maintenance of pig sperm function throughout liquid storage at 17 °C
Authors: Garriga Ferran , Maside Carolina , Padilla Lorena , Recuero Sandra , Rodríguez-Gil Joan E. , Yeste Marc 
Published: June 2024

AbstractAt present, liquid storage is the most efficient method for pig semen preservation. This approach relies upon reducing sperm metabolism, allowing for the maintenance of cell lifespan. In this context, the study of proteins that could protect sperm during liquid storage is of high relevance. The 70 kDa Heat Shock Protein (HSP70) is an anti-apoptotic protein that has been reported to be relevant to sperm survival. Thus, we explored the role of HSP70 during prolonged storage of pig semen at 17 °C. Six semen pools were incubated with YM-1 (0, 0.05, 0.1 and 0.2 μM), an HSP70 inhibitor, and stored at 17 °C for 21 days. On days 0, 4, 10, 14 and 21, sperm quality and function were evaluated through flow cytometry and Computer-Assisted Sperm Analysis (CASA), and HSP70 activity and chromatin condensation were also determined. While inhibition of HSP70 increased progressive motility, Ca2+ and Reactive Oxygen Species (ROS) levels, and mitochondrial activity during the first 10 days of storage, it had a detrimental effect on sperm motility after 14 and 21 days. In spite of this, sperm viability was not altered. We can conclude that HSP70 contributes to the liquid storage of pig semen because it keeps mitochondrial activity low, which is needed for the maintenance of sperm function.
Anomalously weak intensity of tropical cyclones striking eastern China over the past two millennia
Authors: Yang Yang , Maselli Vittorio , Zhou Liang , Gao Jianhua , Gao Ya , Wang Ya Ping , Jia Jianjun , Gao Shu 
Published: June 2024

AbstractAnthropogenic climate warming is predicted to increase the intensity of global tropical cyclones (TCs) on decadal timescales, known as the ‘temperature-TC intensity’ paradigm. However, no proxy is currently available to directly quantify TC intensity in the northwestern Pacific region over centennial to millennial timescales. Here, we reconstruct the intensity of past TCs inferred from event-beds detected in two sedimentary systems in eastern China spanning approximately 1910 to 645 yr BP using an instrumental-calibrated technique, thereby encompassing a sufficiently wide range of temperatures to test the paradigm in the time domain. Intriguingly, our two intensity indices, based on flooding depth and wind speed, provide the initial quantitative evidence that TC intensity in eastern China has been anomalously weak since around 1485 ± 45 yr BP, with a reduction of approximately 30 ± 8% in intensity, despite no concurrent temperature shift. This reduction appears to have been pre-conditioned by a combined influence of a weaker El Niño-Southern Oscillation, a stronger Atlantic Meridional Overturning Circulation, and an increased level of Saharan dust. We suggest that the magnitudes of these factors may have crossed a tipping point and have not reverted to their pre-shift levels since that time, resulting in their impact on TC intensity exceeding that of temperature by triggering changes in the oceanic and atmospheric state within the tropical Pacific region where TCs originate.
Spaceflight induces changes in gene expression profiles linked to insulin and estrogen
Authors: Mathyk Begum Aydogan , Tabetah Marshall , Karim Rashid , Zaksas Victoria , Kim JangKeun , Anu R. I. , Muratani Masafumi , Tasoula Alexia , Singh Ruth Subhash , Chen Yen-Kai , Overbey Eliah , Park Jiwoon , Cope Henry , Fazelinia Hossein , Povero Davide , Borg Joseph , Klotz Remi V. , Yu Min , Young Steven L. , Mason Christopher E. , Szewczyk Nathaniel , St Clair Riley M. , Karouia Fathi , Beheshti Afshin 
Published: June 2024

AbstractOrganismal adaptations to spaceflight have been characterized at the molecular level in model organisms, including Drosophila and C. elegans. Here, we extend molecular work to energy metabolism and sex hormone signaling in mice and humans. We found spaceflight induced changes in insulin and estrogen signaling in rodents and humans. Murine changes were most prominent in the liver, where we observed inhibition of insulin and estrogen receptor signaling with concomitant hepatic insulin resistance and steatosis. Based on the metabolic demand, metabolic pathways mediated by insulin and estrogen vary among muscles, specifically between the soleus and extensor digitorum longus. In humans, spaceflight induced changes in insulin and estrogen related genes and pathways. Pathway analysis demonstrated spaceflight induced changes in insulin resistance, estrogen signaling, stress response, and viral infection. These data strongly suggest the need for further research on the metabolic and reproductive endocrinologic effects of space travel, if we are to become a successful interplanetary species.
High-precision Helicobacter pylori infection diagnosis using a dual-element multimodal gas sensor array
Authors: Wu Jiaying , Xu Shiyuan , Liu Xuemei , Zhao Jingwen , He Zhengfu , Pan Aiwu , Wu Jianmin 
Published: June 2024

A non-invasive, label-free and high-precision diagnosis of Helicobacter pylori (H. pylori) infection is conducted by using a dual-element multimodal gas sensor array.
Consideration of Demographic Variables in Behavioral Interventions Pertaining to Children With Hearing Loss: A Systematic Review
Authors: Bharadwaj Sneha V. , Rhoades Ellen A. , Perry Haley 
Published: June 2024

Purpose:
Equitable representation of children with hearing loss who are members of marginalized or minority groups in behavioral intervention studies enhances inclusivity in the scientific process and generalizability of results. The goal of this systematic review was to ascertain the percentage of studies conducted in the United States in the past 2 decades that reported relevant demographic variables.

Method:
Studies were searched across eight databases and clinical trial registries in October 2022. Variations of the following search strings were used to retrieve peer-reviewed published studies and unpublished clinical trials: children, hearing loss, and intervention.

Results:
Thirty-nine intervention studies met the inclusion criteria. The selected studies were reviewed and coded for the following demographic variables: area and type of intervention, participant age, hearing technology data, sample size, gender, race/skin color and ethnicity, primary/home language(s), additional disabilities, parental education, family income, and parental occupation. Results revealed that many demographic variables were remarkably underreported, with parental education, family income, and parental occupation variables being the least reported data.

Conclusions:
Demographic data can be an important tool for changing disparities related to intervention outcomes. This systematic review suggests that inclusive research practices should be extended to low-literacy or low–economic resources, non-White, and non–English-speaking groups. Inclusion practices coupled with sufficient sample sizes will ultimately aid in identifying hearing health disparities.
Enhancing Adult Hearing Screening Skills for Speech-Language Pathology Students Through Simulation
Authors: Tinsley Jordan , Mitchell Mary P. , Stead Amanda , Michael Paul , DiMicco AJ 
Published: June 2024

Purpose:
This study evaluated student performance and perception of a simulated hearing screening utilizing an adult standardized patient (SP). It explored the relationship between student performance and student perception of the simulation.

Method:
Sixty-nine first-year speech pathology graduate students completed a high-fidelity simulation to demonstrate competency in hearing screening administration with an adult SP. Student performance was assessed by supervising educators through standardized rubrics in the following domains: (a) explaining the task, (b) following the university screening protocol, (c) demonstrating clinical and critical thinking, and (d) using the audiometer efficiently. Students also completed surveys to provide feedback regarding their simulation experience.

Results:

The median rating for student performance across the four domains evaluated was determined using nonparametric tests, while a one-sample
t
test was used to determine average ratings. Additionally, central tendency and dispersion measures were computed using rubric ratings of students' performance in each domain evaluated. Overall, students performed well in this simulation, with nearly all students demonstrating competency in explaining the task and demonstrating clinical/critical thinking. Following the screening protocol and using the audiometer efficiently showed the most room for improvement. Student survey responses generally indicated that this was a positive and beneficial learning experience and provided constructive feedback for future iterations of this simulation.

Conclusions:
Hearing screening simulations effectively train graduate speech-language pathology students in the necessary skills. Student perceptions of this simulation were resoundingly positive.