bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disruption of tight junction structure in salivary glands from Sjögren's syndrome patients is linked to proinflammatory cytokine exposure
Authors: Ewert Patricia , Aguilera Sergio , Alliende Cecilia , Kwon Yoon‐Jeoung , Albornoz Amelina , Molina Claudio , Urzúa Ulises , Quest Andrew F. G. , Olea Nancy , Pérez Paola , Castro Isabel , Barrera María‐José , Romo Rafael , Hermoso Marcela , Leyton Cecilia , González María‐Julieta 
Published: January 2010

AbstractObjectiveDisorganization of acinar cell apical microvilli and the presence of stromal collagen in the acinar lumen suggest that the labial salivary gland (LSG) barrier function is impaired in patients with Sjögren's syndrome. Tight junctions define cell polarity and regulate the paracellular flow of ions and water, crucial functions of acinar cells. This study was undertaken to evaluate the expression and localization of tight junction proteins in LSGs from patients with SS and to determine in vitro the effects of tumor necrosis factor α (TNFα) and interferon‐γ (IFNγ) on tight junction integrity of isolated acini from control subjects.MethodsTwenty‐two patients and 15 controls were studied. The messenger RNA and protein levels of tight junction components (claudin‐1, claudin‐3, claudin‐4, occludin, and ZO‐1) were determined by semiquantitative reverse transcriptase–polymerase chain reaction and Western blotting. Tight junction protein localization was determined by immunohistochemistry. Tight junction ultrastructure was examined by transmission electron microscopy. Isolated acini from control subjects were treated with TNFα and IFNγ.ResultsSignificant differences in tight junction protein levels were detected in patients with SS. ZO‐1 and occludin were strongly down‐regulated, while claudin‐1 and claudin‐4 were overexpressed. Tight junction proteins localized exclusively to apical domains in acini and ducts of LSGs from controls. In SS patients, the ZO‐1 and occludin the apical domain presence of decreased, while claudin‐3 and claudin‐4 was redistributed to the basolateral plasma membrane. Exposure of isolated control acini to TNFα and IFNγ reproduced these alterations in vitro. Ultrastructural analysis associated tight junction disorganization with the presence of endocytic vesicles containing electron‐dense material that may represent tight junction components.ConclusionOur findings indicate that local cytokine production in LSGs from SS patients may contribute to the secretory gland dysfunction observed in SS patients by altering tight junction integrity of epithelial cells, thereby decreasing the quality and quantity of saliva.
Comparison of lateral thermal spread using monopolar and bipolar diathermy, the Harmonic Scalpel™ and the Ligasure™
Authors: Sutton P A , Awad S , Perkins A C , Lobo D N 
Published: January 2010

Abstract

Background
Electrosurgery for dissection and haemostasis should be associated with minimal thermal spread to surrounding tissues. This study investigated lateral thermal spread following ex vivo application of four commonly utilized instruments.

Methods
Monopolar and bipolar diathermy (power settings 20, 30 and 40 W), the Harmonic Scalpel™ and Ligasure™ (power settings 1, 3 and 5) were studied after application to standardized porcine muscle cuts for 5, 10 or 15 s. Temperatures generated at the tips of the instruments, in the tissues adjacent to the tips and 1 cm away were recorded.

Results
Following a 5-s application at the highest power setting, the highest mean(s.d.) temperatures recorded at the tips of monopolar and bipolar diathermy, Harmonic Scalpel™ and Ligasure™ instruments were 78·9(4·1), 41·9(2·2), 47·6(2·5) and 44·2(2·6) °C respectively. Temperatures at the instrument tips after use for 15 s remained above 42 °C for 55, 25, 15 and 15 s respectively. Applying monopolar diathermy (10 s at 40 W) resulted in a temperature recording of 59·2(2·2) °C in tissues 1 cm away from the tip of the instrument.

Conclusion
The degree of lateral thermal spread varied with instrument type, power setting and application time. Monopolar diathermy resulted in the highest temperatures and the greatest degree of thermal spread in tissues.
Nipple-sparing mastectomy
Authors: Rusby J E , Smith B L , Gui G P H 
Published: January 2010

Abstract

Background
Although effective local control is the primary goal of surgery for breast cancer, the long-term aesthetic outcome is also important. Nipple-sparing mastectomy aims to address this, but there is no consensus on its clinical application. Evidence relating to oncological safety, surgical technique and early data on aesthetic outcome was reviewed.

Methods
The review was based on a PubMed search using the terms ‘nipple-sparing’ or ‘subcutaneous mastectomy’ and ‘breast cancer’.

Results
Large pathological studies report occult nipple involvement with cancer in 5·6–31 per cent, reflecting variation in inclusion criteria. Recent clinical series with careful patient selection report local recurrence in less than 5 per cent of patients. The incidence of cancer in the retained nipple after risk-reducing mastectomy is less than 1 per cent. Nipple necrosis rates range up to 8 and 16 per cent for total and partial necrosis respectively. Variations in outcome result from differences in extent of resection, placement of incisions and type of breast reconstruction.

Conclusion
Nipple-sparing mastectomy is an acceptable technique for women undergoing risk-reducing mastectomy. In the therapeutic setting, it may be offered to patients with smaller tumours far from the nipple and favourable pathological features. Women should be counselled about nipple necrosis and the potential for local recurrence.
Application of a rapid and selective method for the simultaneous determination of carebastine and pseudoephedrine in human plasma by liquid chromatography–electrospray mass spectrometry for bioequivalence study in Korean subjects
Authors: Lee Myung‐Jae , Lee Heon‐Woo , Kang Jong‐Min , Seo Ji‐Hyung , Tak Seong‐Kun , Shim Wangseob , Yim Sung‐Vin , Hong Seung Jae , Lee Kyung‐Tae 
Published: January 2010

AbstractWe describe a simple, rapid and sensitive high‐performance liquid chromatography–electrospray ionization tandem mass spectrometric method that was developed for the simultaneous determination of carebastine and pseudoephedrine in human plasma using cisapride as an internal standard. Acquisition was performed in multiple‐reaction monitoring mode by monitoring the transitions: m/z 500.43 > 167.09 for carebastine and m/z 166.04 > 147.88 for pseudoephedrine. The devised method involves a simple single‐step liquid–liquid extraction with ethyl acetate. Chromatographic separation was performed on a C18 reversed‐phase chromatographic column at 0.2 mL/min by isocratic elution with 10 mM ammonium formate buffer–acetonitrile (30:70, v/v; adjusted to pH 3.3 with formic acid). The devised method was validated over 0.5–100 ng/mL of carebastine and 5–1000 ng/mL of pseudoephedrine with acceptable accuracy and precision, and was successfully applied to a bioequivalence study involving a single oral dose (10 mg of ebastine plus 120 mg of pseudoephedrine complex) to healthy Korean volunteers. Copyright © 2010 John Wiley & Sons, Ltd.
Searching for the developmental origins of sexual violence: examining the co‐occurrence of physical aggression and sexual behaviors in early childhood
Authors: Lussier Patrick , Healey Jay 
Published: January 2010

AbstractWhile developmental perspectives on sexual violence have gained much interest in recent years, few empirical studies have been conducted to better understand its origins. This study attempts to fill this gap by examining the onset of physical aggression and normative sexual behaviors in preschoolers. This study is based on a sample of at‐risk children (n = 100) recruited as part of the KD‐BEAR project, an on‐going longitudinal study conducted in Vancouver, British Columbia, Canada. Semi‐structured interviews were completed with the primary caregiver and the child. The structural model examined showed a significant and important latent correlation between physical aggression and sexual behaviors across models tested, after controlling for child and familial characteristics. Furthermore, findings showed that male preschoolers coming from low income families having been referred for assessment and/or treatment for an externalizing spectrum disorder showed higher levels of both aggression and sexual behaviors. The implications of these findings are discussed in light of developmental models of sexual violence, and the secondary prevention of sexual violence at its earliest stages. Copyright © 2010 John Wiley & Sons, Ltd.
Biodisk: A new device for closure of patent foramen ovale: A feasibility study in swine
Authors: Pavcnik Dusan , Takulve Kurt , Uchida Barry T. , Pavcnik Arnol Maja , VanAlstine William , Keller Frederick , Rösch Josef 
Published: January 2010

AbstractPurpose: To evaluate the feasibility, effectiveness, and safety of a porcine small intestinal submucosa (SIS)‐covered Biodisk (BD) for the closure of patent foramen ovale (PFO) in swine. Methods: Twelve piglets (9–30 kg) with PFO ranging in size from 6 to 12 mm were used for the in vivo testing. The BD device consisted of two basic nitinol wire components covered with platinum coil, a flexible SIS‐covered ring, and an anchor. The BD was advanced through an 8‐Fr sheath from the femoral vein. Nine acute animals were used to test the BD for deployment, stability, immediate shunt closure, and device repositioning before or after its detachment. To assess retrievability, four devices were deployed and intentionally embolized into the RA (n = 2) and LA (n = 2). The effectiveness of the device was evaluated by angiocardiography. EKG was recorded before and after PFO closure for 3 hr. From the 12 animals, nine were acute and three were followed; one for 6 weeks, one for 12 weeks, and one for 16 weeks. Results: Successful device implantation was achieved in all animals with no shunting of contrast media observed during follow‐up in. One animal needed to have device repositioned for complete PFO occlusion because of suboptimal placement at the first attempt. The device was easily placed and retrieved before detachment in all nine animals in the acute study. None of the BDs spontaneously embolized during release or on follow‐up. EKG did not demonstrate arrhythmias during or after treatment. Four intentionally embolized BDs were easily retrieved with an Amplatz goose neck snare. Macroscopic and histologic evaluation of the three long‐term animals showed that devices were well incorporated in the atrial septum with complete shunt closure. The SIS showed progressive remodeling with the host cells. There was also progressive endothelization of the BD device. Conclusion: The BD device deployment is feasible, safe, and effective. Long‐term studies are needed to evaluate its long‐term effectiveness. © 2010 Wiley‐Liss, Inc.
A noncompliant, high pressure balloon to manage undilatable coronary lesions
Authors: Raja Yogesh , Routledge Helen C. , Doshi Sagar N. 
Published: January 2010

AbstractUndilatable coronary lesions, due to coronary calcification, are encountered with increasing frequency. Percutaneous intervention of such lesions comes with the risk of stent underexpansion, which increases the risk of stent thrombosis and restenosis. A variety of techniques and devices are available to assist the interventionist in the treatment of calcified lesions. The OPN NC (SIS‐Medical AG, Winterthur, Switzerland) is a high‐pressure balloon (rated burst pressure 35 atm) that uses a novel, twin‐layer balloon technology to ensure uniform balloon expansion, eliminating “dog‐boning.” We report four cases of successful treatment of calcified lesions with the OPN NC balloon after dilatation with standard noncompliant balloons to high‐pressure failed. © 2010 Wiley‐Liss, Inc.
Patients with coronary artery disease not amenable to traditional revascularization: Prevalence and 3‐year mortality
Authors: Williams Benjamin , Menon Madhav , Satran Daniel , Hayward Daniel , Hodges James S. , Burke M. Nicholas , Johnson Randall K. , Poulose Anil K. , Traverse Jay H. , Henry Timothy D. 
Published: January 2010

AbstractObjectives: To determine the contemporary prevalence of and mortality in patients with coronary artery disease (CAD) not amenable to revascularization. Background: A growing number of patients have severe CAD with ongoing angina despite optimal medical therapy which is not amenable to traditional revascularization. Limited data exist on contemporary prevalence and outcome for these patients. Methods: Clinical and angiographic data were reviewed for 493 consecutive patients undergoing coronary angiography and revascularization if indicated. Patients were categorized into six groups: (1) normal coronary arteries, (2) CAD <70%, (3) CAD >70% with complete revascularization by percutaneous intervention or coronary artery bypass grafting, (4) CAD >70% with partial revascularization, (5) CAD >70% treated medically, and (6) CAD >70% on optimal medical therapy with no revascularization option. All‐cause mortality at 3 years was determined. Results: Prevalence for groups 1–6 was 14.8, 19.5, 36.9, 12.8, 9.3, and 6.7%, respectively. Three‐year mortality increased with angiographic severity of CAD: 2.7, 6.3, 8.2, 12.7, 17.4, and 15.2%, respectively. Patients with incomplete revascularization (groups 4–6, n = 142) had higher mortality than completely revascularized patients (groups 1–3, n = 351): 14.8 vs. 6.6% (P = 0.004). Conclusions: In a contemporary series of patients undergoing coronary angiography, 28.8% (142/493) of patients had significant CAD and did not undergo complete revascularization, including 12.8% partially revascularized, 9.3% managed medically, and 6.7% with “no‐option.” These patients had higher mortality at 3 years (14.8 vs. 6.6%, P = 0.004) when compared with completely revascularized patients. © 2010 Wiley‐Liss, Inc.
Treatment of an iatrogenic subclavian artery dissection
Authors: Spies Christian , Fergusson David 
Published: January 2010

AbstractSubclavian artery dissection is a rare complication of cardiac catheterization. The usual reason for entering this vessel is to access the origin of the left internal mammary artery (LIMA) when this has previously been used, or is being considered, as a bypass graft. We report an instance of such dissection occurring long after bypass surgery which had included a LIMA graft. Successful correction was achieved by angioplasty and stent placement, using a retrograde left brachial approach. © 2010 Wiley‐Liss, Inc.
A prospective multicenter registry of 0.010‐inch guidewire and compatible system for chronic total occlusion: The PIKACHU registry
Authors: Matsukage Takashi , Masutani Motomaru , Yoshimachi Fuminobu , Takahashi Akihiko , Katsuki Takaaki , Saito Shigeru , Terai Hidenobu , Katahira Yoshiaki , Uehara Yoshiki , Tohara Satoru , Ohba Yurika , Shinohara Shoichi , Asano Hiroshi , Matsumura Toshiyuki , Hata Tetsuya , Ikari Yuji 
Published: January 2010

AbstractObjectives: The aim of this study was to evaluate the safety and effectiveness of a 0.010‐inch guidewire and a balloon catheter for treatment of chronic total occlusion (CTO). Background: Pathological studies have shown that 60–70% of CTO lesions have microchannels of sizes equal to or less than 0.010 inch. Methods: The PIKACHU registry is a prospective, multicenter registry study. A 0.010‐inch guidewire had to be used as the first guidewire to attempt to pass the CTO lesion. The primary endpoint was device success using a 0.010 system. Results: A total of 141 patients with 141 lesions were enrolled. The median duration of occlusion was 9 months (range 3–156). Average guiding catheter size was 5.8 ± 0.7 Fr. and TRI was 76.6 %. CTOs were mostly between 10–20 mm long, observed in 53 occlusions. There were 107 lesions (75.9%) with bending of more than 45 degrees. Calcification was seen in 91 lesions (64.5%). A 0.010‐inch guidewire was successfully passed through in 97 of 141 lesions (68.8%). A 0.010‐inch guidewire compatible balloon catheter was passed in 87 of the 97 lesions (88.7%) and final PCI success was achieved in all the cases. The overall clinical success rate was 87.9% (124/141). No MACE or bleeding complications were observed. Conclusion: The PIKACHU registry data suggest that the 0.010‐inch system is safe and practicable for treatment of CTO lesions. © 2010 Wiley‐Liss, Inc.
Atrial myxoma's and coronary angiography
Authors: Rafiq Isma , Parthasarthy H. , Clark C. Grahame 
Published: January 2010

AbstractCoronary angiography is not an only important component of preoperative evaluation of the patient with underlying coronary artery disease but also diagnostic tool for delineating cardiac myxomas. This also serve as an important surgical anatomical marker. We present two cases which presented with repeated episode of chest pain, were found to have atrial blushing on coronary angiography subsequent confirmation of diagnosis of atrial myxoma on echocardiography. © 2010 Wiley‐Liss, Inc.
Rotational atherectomy for fibro‐calcific coronary artery disease in drug eluting stent era: Procedural outcomes and angiographic follow‐up results
Authors: Rathore Sudhir , Matsuo Hitoshi , Terashima Mitsuyasu , Kinoshita Yoshihisa , Kimura Masashi , Tsuchikane Etsuo , Nasu Kenya , Ehara Mariko , Asakura Yasushi , Katoh Osamu , Suzuki Takahiko 
Published: January 2010

AbstractObjectives: The aim of this study was to examine the binary re‐stenosis rates, procedural success, and in hospital outcomes following treatment of fibro‐calcified coronary lesion with rotational atherectomy in drug eluting stent era. Background: Binary restenosis rates have remained high with the use of bare metal stents following rotational atherectomy in calcified lesions. There is limited data available following rotational atherectomy in drug eluting stent era. Methods: We evaluated the procedural and angiographic outcomes following a consecutive series of 516 procedures treated with rotational atherectomy followed by stenting. We compared the results between Rota + Drug eluting stent (DES) and Rota + bare metal stent (BMS) groups. Results: Procedural success was achieved in 97.1% of the lesions with overall low in hospital adverse events (death in 1.1%, Q MI in 1.3%, Non Q MI in 5.3%, and urgent repeat PCI in 0.4%). There was significant reduction in the binary restenosis rates following Rota + DES use as compared to Rota + BMS use (11% vs. 28.1%, P < 0.001; OR = 3.17, 95% CI: 1.76–5.93) and similar reduction was seen in the target lesion revascularization (10.6% vs. 25%, P = 0.001; OR = 2.81, 95% CI: 1.53–5.14). We have identified ostial lesions, chronic total occlusion lesions, and use of bare metal stents as independent predictors of restenosis in this group of patients. Conclusions: Rotational atherectomy can be performed with high success rates and low complications, and rotational atherectomy followed by drug eluting stent implantation significantly reduces binary restenosis rates in fibrocalcific lesions as compared to rotational atherectomy and bare metal stents. © 2010 Wiley‐Liss, Inc.
Contemporary use of embolic protection devices in saphenous vein graft interventions: Insights from the stenting of saphenous vein grafts trial
Authors: Badhey Neeraj , Lichtenwalter Christopher , de Lemos James A. , Roesle Michele , Obel Owen , Addo Tayo A. , Haagen Donald , Abdel‐Karim Abdul‐Rahman , Saeed Bilal , Bissett Joseph K. , Sachdeva Rajesh , Voudris Vassilios V. , Karyofillis Panagiotis , Kar Biswajit , Rossen James , Fasseas Panayotis , Berger Peter B. , Banerjee Subhash , Brilakis Emmanouil S. 
Published: January 2010

AbstractBackground: We sought to evaluate the contemporary use of embolic protection devices (EPDs) in saphenous vein graft (SVG) interventions. Methods: We examined EPD use in the stenting of saphenous vein grafts (SOS) trial, in which 80 patients with 112 lesions in 88 SVGs were randomized to a bare metal stent (39 patients, 43 grafts, and 55 lesions) or paclitaxel‐eluting stent (41 patients, 45 grafts, and 57 lesions). Results: An EPD was used in 60 of 112 lesions (54%). A Filterwire (Boston Scientific) was used in 70% of EPD‐treated lesions, Spider (ev3, Plymouth, Minnesota) in 12%, Proxis (St. Jude, Minneapolis, Minnesota) in 12%, and Guardwire (Medtronic, Santa Rosa, California) in 7%. Of the remaining 52 lesions, an EPD was not utilized in 13 lesions (25%) because the lesion was near the distal anastomosis, in 14 lesions (27%) because of an ostial location, in one lesion (2%) because of small SVG size, in two in‐stent restenosis lesions (4%) because of low distal embolization risk, and in 22 lesions (42%) because of operator's preference even though use of an EPD was feasible. Procedural success was achieved in 77 patients (96%); in one patient a Filterwire was entrapped requiring emergency coronary bypass graft surgery and two patients had acute stent thrombosis. Conclusion: In spite of their proven efficacy, EPDs were utilized in approximately half of SVG interventions in the SOS trial. Availability of a proximal protection device could allow protection of ∼25% of unprotected lesions, yet operator discretion appears to be the major determinant of EPD use. © 2010 Wiley‐Liss, Inc.
Outcomes after differential use of drug‐eluting stents in diabetic patients: 1‐Year results from the DES.DE (Drug‐Eluting Stent.DEutschland) registry
Authors: Akin Ibrahim , Bufe Alexander , Eckardt Lars , Reinecke Holger , Richardt Gert , Kuck Karl‐Heinz , Senges Jochen , Schneider Steffen , Nienaber Christoph A. ,   for the DES.DE Study group
Published: January 2010

AbstractObjectives: The prospective multicenter German DES.DE registry provides real world data to evaluate the therapeutic principle of two different drug‐eluting stents (DES) [Sirolimus‐ (SES) and Paclitaxel‐eluting stent (PES)] in the context of the German Health System. Background: Differential DES have been effective in randomized trials, but their difference in safety and efficacy in diabetic patients has not been well studied. Methods: Baseline, predefined procedural as well as clinical in‐hospital and follow‐up events were recorded for all 1,526 diabetic patients. The composite of death, myocardial infarction (MI), and stroke defined as major adverse cardiac and cerebrovascular events (MACCE) and target vessel revascularization (TVR) were defined as primary endpoints. Results: Between October 2005 and October 2006, 1,526 diabetic patients, 34.2% of them being insulin‐dependent, were enrolled (SES: n = 612; PES: n = 914) at 98 DES.DE sites. Overall, one third of patients were admitted with acute coronary syndrome (ACS) and 70% had multivessel‐disease reflecting a real world scenario. With similar baseline clinical and descriptive morphology of coronary artery disease (CAD) in both DES groups, there were no statistical differences in 1‐year follow‐up with respect to rates of overall mortality (5.8% vs. 5.4%), TVR (12.0% vs. 11.3%), overall stent thrombosis (5.6% vs. 4.6%) and MACCE (11.4% vs. 10.3%) between both DES. Conclusion: The data collected in DES.DE revealed no differences in clinical outcomes within 1 year between SES and PES in diabetic patients in a “real‐world” setting. © 2010 Wiley‐Liss, Inc.