bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alveolar ridge augmentation using implants coated with recombinant human growth/differentiation factor‐5: histologic observations
Authors: Polimeni Giuseppe , Wikesjö Ulf M. E. , Susin Cristiano , Qahash Mohammed , Shanaman Richard H. , Prasad Hari S. , Rohrer Michael D. , Hall Jan 
Published: May 2010

Polimeni G, Wikesjö UME, Susin C, Qahash M, Shanaman RH, Prasad HS, Rohrer MD, Hall J. Alveolar ridge augmentation using implants coated with recombinant human growth/differentiation factor‐5: histologic observations. J Clin Periodontol 2010; 37: 759‐768 doi: 10.1111/j.1600-051X.2010.01579.x.AbstractObjectives: In vitro and in vivo preclinical studies suggest that growth/differentiation factor‐5 (GDF‐5) may induce local bone formation. The objective of this study was to evaluate the potential of recombinant human GDF‐5 (rhGDF‐5) coated onto an oral implant with a purpose‐designed titanium porous oxide surface to stimulate local bone formation including osseointegration and vertical augmentation of the alveolar ridge.Materials and Methods: Bilateral, critical‐size, 5 mm, supraalveolar peri‐implant defects were created in 12 young adult Hound Labrador mongrel dogs. Six animals received implants coated with 30 or 60 μg rhGDF‐5, and six animals received implants coated with 120 μg rhGDF‐5 or left uncoated (control). Treatments were alternated between jaw quadrants. The mucoperiosteal flaps were advanced, adapted, and sutured to submerge the implants for primary intention healing. The animals received fluorescent bone markers at weeks 3, 4, 7, and 8 post‐surgery when they were euthanized for histologic evaluation.Results: The clinical examination showed no noteworthy differences between implants coated with rhGDF‐5. The cover screw and implant body were visible/palpable through the alveolar mucosa for both rhGDF‐5‐coated and control implants. There was a small increase in induced bone height for implants coated with rhGDF‐5 compared with the control, induced bone height averaging (±SD) 1.6±0.6 mm for implants coated with 120 μg rhGDF‐5 versus 1.2±0.5, 1.2±0.6, and 0.6±0.2 mm for implants coated with 60 μg rhGDF‐5, 30 μg rhGDF‐5, or left uncoated, respectively (p<0.05). Bone formation was predominant at the lingual aspect of the implants. Narrow yellow and orange fluorescent markers throughout the newly formed bone indicate relatively slow new bone formation within 3–4 weeks. Implants coated with rhGDF‐5 displayed limited peri‐implant bone remodelling in the resident bone; the 120 μg dose exhibiting more advanced remodelling than the 60 and 30 μg doses. All treatment groups exhibited clinically relevant osseointegration.Conclusions: rhGDF‐5‐coated oral implants display a dose‐dependent osteoinductive and/or osteoconductive effect, bone formation apparently benefiting from local factors. Application of rhGDF‐5 appears to be safe as it is associated with limited, if any, adverse effects.
Detection of free immunoglobulin light chains in cerebrospinal fluids of patients with central nervous system lymphomas
Authors: Schroers Roland , Baraniskin Alexander , Heute Christoph , Kuhnhenn Jan , Alekseyev Andriy , Schmiegel Wolff , Schlegel Uwe , Pels Hendrik‐Johannes 
Published: May 2010

AbstractDiagnosis of central nervous system (CNS) lymphoma depends on histopathology of brain biopsies, because no reliable disease marker in the cerebrospinal fluid (CSF) has been identified yet. B‐cell lymphomas such as CNS lymphomas are clonally restricted and express either kappa or lambda immunoglobulin light chains. The aim of this study was to find out a potential diagnostic value of free immunoglobulin light chains released into the CSF of CNS lymphoma patients. Kappa (κ) and lambda (λ) free immunoglobulin light chains (FLC) were measured in CSF and serum samples collected from 21 patients with primary and secondary CNS lymphomas and 14 control patients with different neurologic disorders. FLC concentrations and ratios were compared between patient groups and were further analyzed in correlation with clinical, cytopathological, and radiological findings. FLC concentrations for all patients were lower in CSF when compared to serum. In patients with CNS lymphoma, the FLC ratios in CSF were higher (range 392–0.3) compared to control patients (range 3.0–0.3). Irrespective of cytopathological proven lymphomatous meningitis, in 11/21 lymphoma CSF samples the FLC ratios were markedly above 3.0 indicating a clonally restricted B‐cell population. Increased FLC ratios in CSF were found in those patients showing subependymal lymphoma contact as detected in magnetic resonance imaging. In summary, this is the first report demonstrating that a significant proportion of patients with CNS lymphomas display a markedly increased FLC ratio in the CSF.
PGE2 is a UVR‐inducible autocrine factor for human melanocytes that stimulates tyrosinase activation
Authors: Starner Renny J. , McClelland Lindy , Abdel‐Malek Zalfa , Fricke Alex , Scott Glynis 
Published: May 2010

Please cite this paper as: PGE2 is a UVR‐inducible autocrine factor for human melanocytes that stimulates tyrosinase activation. Experimental Dermatology 2010; 19: 682–684.Abstract:  Prostaglandins activate signalling pathways involved in growth, differentiation and apoptosis. Prostaglandin E2 (PGE2) is released by keratinocytes following ultraviolet irradiation (UVR) and stimulates the formation of dendrites in melanocytes. We show that multiple irradiations of human melanocytes with UVR‐activated cPLA2, the rate‐limiting enzyme in eicosanoid synthesis and stimulated PGE2 secretion. PGE2 increased cAMP production, tyrosinase activity and proliferation in melanocytes. PGE2 binds to four distinct G‐protein coupled receptors (EP1–4). We show that PGE2 stimulates EP4 receptor signalling in melanocytes, resulting in cAMP production. Conversely, PGE2 also stimulated the EP3 receptor in melanocytes, resulting in lowered basal cAMP levels. These data suggest that relative levels or activity of these receptors controls effects of PGE2 on cAMP in melanocytes. The data are the first to identify PGE2 as an UVR‐inducible autocrine factor for melanocytes. These data also show that PGE2 activates EP3 and EP4 receptor signalling, resulting in opposing effects on cAMP production, a critical signalling pathway that regulates proliferation and melanogenesis in melanocytes.
IgA autoantibodies in the pemphigoids and linear IgA bullous dermatosis
Authors: Horváth Barbara , Niedermeier Andrea , Podstawa Eva , Müller Ralf , Hunzelmann Nicolas , Kárpáti Sarolta , Hertl Michael 
Published: May 2010

Please cite this paper as: IgA autoantibodies in the pemphigoids and linear IgA bullous dermatosis. Experimental Dermatology 2010; 19: 648–653.Background:  Patients with bullous pemphigoid (BP), mucous membrane pemphigoid (MMP) and pemphigoid gestationis (PG) have IgG antibodies against BP180 and BP230, components of the hemidesmosomes. Patients with linear IgA bullous dermatosis (LABD) have IgA autoantibodies against a 97/120‐kDa protein which is highly homologous to a shedded fragment of the BP180‐ectodomain.Objectives:  The aim of our study was to determine the incidence of IgA autoantibodies directed against BP180/BP230 in the pemphigoids and LABD and to determine the antigenic regions that are targeted by IgA autoantibodies.Methods:  Utilizing baculovirus‐expressed recombinant BP180 and BP230 proteins, we performed immunoblot analyses for IgA reactivity of sera from patients with BP (n = 30), MMP (n = 10), PG (n = 6), LABD (n = 6) and from control patients with non‐related pruritic dermatoses (n = 8).Results:  IgA reactivity against BP180 and/or BP230 was detected in 19/30 of the BP, in 7/10 of the MMP, in 6/6 of the LABD and in 3/6 of the PG sera, respectively, but not in the control group. In all subgroups, the major antigenic site recognized by IgA antibodies was located within the NH2‐terminus of the BP180‐ectodomain, but only a minority of the sera showed also IgA reactivity against the BP180‐NC16a‐domain. IgA reactivity against the central domain of BP180 was more frequently seen than against its COOH‐terminus. IgA against the COOH‐ and NH2‐terminus of BP230, respectively, was detected in 6/30 of the BP, 1/10 of the MMP, 1/6 of the LABD and 0/8 control sera.Conclusion:  IgA reactivity against BP180 and/or BP230 is a common finding in the pemphigoids.
Joining the Immunological Dots in Recurrent Miscarriage
Authors: Bansal Amolak Singh 
Published: May 2010

Citation Bansal AS. Joining the immunological dots in recurrent miscarriage. Am J Reprod Immunol 2010AbstractWhile raised cellular immunity mediated by T helper (Th) 1 type cells may be harmful for the developing embryo/foetus, it is likely that Th2 type immunity may be helpful. The role of natural killer (NK) cells is presently underestimated, although they are clearly important in angiogenesis and the coordinated invasion of the decidua by the trophoblast. Deficient T regulatory cell (Treg) function is evident in women with recurrent miscarriage particularly when this occurs in early pregnancy. The role of the pro‐inflammatory Th17 cells is presently unclear. However, early evidence suggests that excessive Th17 activity may promote miscarriage and preterm delivery. This may relate to the ability of these cells to produce those cytokines that encourage Th1 and NK cell activity. As such recurrent miscarriage may be caused not only by chromosomal abnormalities, autoimmunity and uterine abnormalities but also by subclinical uterine infection and inflammation which by stimulating interleukin 6 favours Th17 development over Tregs. This review examines the role of these different cells in early pregnancy and suggests a schema that may join the dots of the immunological puzzle called pregnancy. Finally, suggestions are made as to how inappropriate immunity in recurrent miscarriage may be down‐regulated using currently available therapies.
Women's Sense of Coherence related to their infant feeding experiences
Authors: Thomson Gill , Dykes Fiona 
Published: May 2010

AbstractGiven the overwhelming evidence for the benefits of breastfeeding, and yet the low prevalence rates in the UK, it is crucial to understand the influences on women's infant feeding experiences to target and promote effective support. As part of an evaluation study of the implementation of the UNICEF UK Baby Friendly Initiative (BFI) Community Award within two primary (community‐based) care trusts in North West England, 15 women took part in an in‐depth interview to explore their experiences, opinions and perceptions of infant feeding. In this paper, we have provided a theoretical interpretation of these women's experiences by drawing upon Aaron Antonovsky's Sense of Coherence (SOC) theory. The SOC is a global orientation to how people are able to cope with stressors and maintain a sense of well‐being. The three constructs that underpin the SOC are ‘comprehensibility’ (one must believe that one understands the life challenge), ‘manageability’ (one has sufficient resources at one's disposal) and ‘meaningfulness’ (one must want to cope with the life challenge). In this paper, our interpretations explore how infant feeding is influenced by the ‘comprehensibility’, ‘manageability’ and ‘meaningfulness’ of this event; contextualized within a wider socio‐cultural perspective. The findings of this paper offer a unique means through which the influences on women's experiences of infant feeding may be considered. Recommendations and suggestions for practice in relation to the implementation of the BFI have also been presented.
New insights into structure–function relationships of oxalyl CoA decarboxylase from Escherichia coli
Authors: Werther Tobias , Zimmer Agnes , Wille Georg , Golbik Ralph , Weiss Manfred S. , König Stephan 
Published: May 2010

The gene yfdU from Escherichia coli encodes a putative oxalyl coenzyme A decarboxylase, a thiamine diphosphate‐dependent enzyme that is potentially involved in the degradation of oxalate. The enzyme has been purified to homogeneity. The kinetic constants for conversion of the substrate oxalyl coenzyme A by the enzyme in the absence and presence of the inhibitor coenzyme A, as well as in the absence and presence of the activator adenosine 5′‐diphosphate, were determined using a novel continuous optical assay. The effects of these ligands on the solution and crystal structure of the enzyme were studied using small‐angle X‐ray scattering and X‐ray crystal diffraction. Analyses of the obtained crystal structures of the enzyme in complex with the cofactor thiamine diphosphate, the activator adenosine 5′‐diphosphate and the inhibitor acetyl coenzyme A, as well as the corresponding solution scattering patterns, allow comparison of the oligomer structures of the enzyme complexes under various experimental conditions, and provide insights into the architecture of substrate and effector binding sites.Structured digital abstract
MINT‐7717846: EcODC (uniprotkb:P0AFI0) and EcODC (uniprotkb:P0AFI0) bind (MI:0407) by X‐ray scattering (MI:0826)
MINT‐7717834: EcODC (uniprotkb:P0AFI0) and EcODC (uniprotkb:P0AFI0) bind (MI:0407) by X‐ray crystallography (MI:0114)
Effects of Testosterone Supplementation on Depressive Symptoms and Sexual Dysfunction in Hypogonadal Men with the Metabolic Syndrome
Authors: Giltay Erik J. , Tishova Yuliya A. , Mskhalaya George J. , Gooren Louis J.G. , Saad Farid , Kalinchenko Svetlana Y. 
Published: May 2010

ABSTRACT

Introduction
Low testosterone levels in men are associated with the metabolic syndrome (MetS) as well as with depressive symptoms, low vitality, and sexual dysfunction.

Aim
To assess the effects of testosterone administration on these subjective symptoms, which have not extensively been studied in hypogonadal men with the MetS.

Main Outcome Measures
The Beck Depression Inventory (BDI-IA), Aging Males’ Symptoms (AMS) scale, and International Index of Erectile Function 5-item (IIEF-5) scale at baseline, 18 and 30 weeks were analysed using multilevel analysis.

Methods
In a randomized, placebo-controlled, double-blind, phase III trial (ClinicalTrials.gov identifier: NCT00696748), 184 men suffering from both the MetS and hypogonadism were included. They were treated for 30 weeks with either parenteral testosterone undecanoate (TU; 1,000 mg IM TU, at baseline, and after 6 and 18 weeks; Nebido®) or placebo injections, 105 (92.9%) men receiving TU and 65 (91.5%) receiving placebo completed the 30-week trial.

Results
The 184 men were aged mean 52.1 years old (standard deviation [SD] 9.6; range 35–69), with a mean body mass index of 35.5 kg/m2 (SD 6.7; range 25.1–54.8), and a mean total testosterone level of 8.0 nmol/L (SD 4.0). There were significant improvements in BDI-IA (mean difference vs. placebo after 30 weeks: −2.5 points; 95% confidence interval [CI]: −0.9; −4.1; P = 0.003), AMS (−7.4 points; 95% CI: −4.3; −10.5; P &lt;0.001), and IIEF-5 (+3.1 points; 95% CI: +1.8; +4.4; P &lt;0.001). The effects on the BDI-IA, AMS, and IIEF-5 were strongest in men with baseline total testosterone levels &lt;7.7 mmol/L (i.e., median value).

Conclusions
TU administration may improve depressive symptoms, aging male symptoms and sexual dysfunction in hypogonadal men with the MetS. The beneficial effects of testosterone were most evident in men with the lowest baseline total testosterone levels.
Genome‐wide haplotype association analysis and gene prioritization identify CCL3 as a risk locus for rheumatoid arthritis
Authors: Zhang R. , Sun P. , Jiang Y. , Chen Z. , Huang C. , Zhang X. , Zhang R. 
Published: May 2010

SummaryRheumatoid arthritis (RA) is a common autoimmune disease caused by a complex interaction of multiple genetic variants and environmental factors. The association between RA and genetic susceptibility loci has been observed in many different populations, and most studies have focused on univariate analyses of single nucleotide polymorphisms. We performed a genome‐wide haplotype association study and prioritized RA‐related genes within 100 kb in either direction of significant haplotypes (P < 0.0001), based on their similarity to known RA susceptibility genes. The results showed that the chemokine CCL3 was associated with susceptibility to RA. A haplotype that located CCL3 on chromosome 17q12 had significant correlation with RA (P = 7.56E‐05), and the global similarity score of CCL3 was ranked in the top of all RA‐related genes, excluding known susceptibility genes (P = 8.42E‐07). Our findings provide further evidence for the potential importance of the chemokine CCL3 in RA, and will facilitate the further understanding of its role in immunological regulation and the pathogenesis of RA.
Association of longevity with IL‐10 −1082 G/A and TNF‐α−308 G/A polymorphisms
Authors: Khabour O. F. , Barnawi J. M. 
Published: May 2010

SummaryCytokines are crucial for the regulation of inflammation development in humans. Many studies have shown that variations in cytokine genes might play a role in determining human longevity. This study examined the changes in the gene pool relevant to the −308 G/A polymorphism in the promoter region of the proinflammatory cytokine tumour necrosis factor (TNF)‐α gene and the −1082 G/A polymorphism in the promoter region of anti‐inflammatory cytokine interleukin (IL)‐10 gene with aging and survival selection occurs in the Jordanian population. IL‐10 −1028 G/A and TNF‐α−308 G/A were genotyped in 119 randomly selected elderly subjects (41 women and 78 men) with a mean age of 90.2 years and young control subjects of 118 (46 women and 72 men) with a mean age of 31.9 years. No significant differences were found in the genotype and allele frequencies of TNF‐α gene variants between the two groups (P > 0.05) while the IL‐10 genotype and allele frequencies were significantly associated with longevity in men (P < 0.05) but not in women (P < 0.05). Thus, IL‐10 −1028 G/A polymorphism seems to play a role in the pathway to longevity in Jordanian men.
The rate of MEFV gene mutations in hematolymphoid neoplasms
Authors: Celik S. , Erikci A. A. , Tunca Y. , Sayan O. , Terekeci H. M. , Umur E. E. , Torun D. , Tangi F. , Top C. , Oktenli C. 
Published: May 2010

SummaryThe aim of this study was to determine the rate of MEFV gene mutations, the gene responsible for familial Mediterranean fever (FMF), in patients with hematolymphoid neoplasm. The rate of the five most common MEFV gene mutations (M694V, M680I, V726A, M694I and E148Q) was determined in 46 patients with hematolymphoid neoplasm. We found a high frequency of carriers in patients with multiple myeloma (60%) and acute lymphocytic leukaemia (33.3%), whereas patients with chronic lymphocytic leukaemia (9%) and non‐Hodgkin lymphoma (5%) had a low mutation carrier rate. There is no MEFV gene mutation in patients with Hodgkin lymphoma. Furthermore, the statistically significant predominance of strong heterozygous mutations such as M694V and M680I in patients with hematolymphoid neoplasm; none had own and/or family history compatible with FMF, is interesting. In conclusion, we found a high frequency of carriers for MEFV gene in patients with multiple myeloma and acute lymphocytic leukaemia. The data of our study may provide some new insights in understanding of individual genetic differences in susceptibility to these neoplasms.
The Weaver Wasp: Spinning Fungus into a Nest
Authors: Ruiz‐González Mario X. , Corbara Bruno , Leroy Céline , Dejean Alain , Orivel Jérôme 
Published: May 2010

ABSTRACTWasp nests range from simple to complex structures made of paper or mud. Here, we show that a Neotropical wasp of the genusNitelabuilds its nest entirely by weaving endophytic fungal hyphae and spider silk harvested from the leaves growing in the understory of the rain forest in French Guiana.
Natural Hybridization betweenRhododendron delavayiandR. cyanocarpum(Ericaceae), from Morphological, Molecular and Reproductive Evidence
Authors: Ma Yong‐Peng , Zhang Chang‐Qin , Zhang Jing‐Li , Yang Jun‐Bo 
Published: May 2010

AbstractThe natural hybridization that occurs between two sympatric species ofRhododendronsubgenusHymenanthesin Yunnan, China, was investigated. In field observations, it was noted that the putative hybrids betweenR. delavayiFranch. andR. cyanocarpum(Franch.) Franch. ex W.W. Sm. had intermediate morphologies. On the basis of morphology, chloroplast DNA (trnL‐rpl32) and nuclear DNA (waxy), hybrids and parental species were identified. Hybridization occurred in both directions, but was asymmetrical, withR. delavayias the major maternal parent. Reciprocal hand pollination treatments showed that either species, as pollen donor or pollen receiver, could produce fruits. It was noted that fruit set varied among treatments. The same pollinators (bumblebees) were shared in both parental species. From these results, we conclude that individuals with intermediate morphologies are indeed of hybrid origin from natural hybridization betweenR.cyanocarpumandR. delavayi. Furthermore, we presume the hybridization at the study site could have been initiated by habitat disturbance in the 1950s, and we may hence witness the early stages of hybrid swarm formation.
Fast Isolation of Highly Active Photosystem II Core Complexes from Spinach
Authors: Wang Zhao‐Gai , Xu Tian‐Hua , Liu Cheng , Yang Chun‐Hong 
Published: May 2010

Abstract Purification of photosystem II (PSII) core complexes is a time‐consuming and low‐efficiency process. In order to isolate pure and active PSII core complexes in large amounts, we have developed a fast method to isolate highly active monomeric and dimeric PSII core complexes from spinach leaves by using sucrose gradient ultracentrifugation. By using a vertical rotor the process was completed significantly faster compared with a swing‐out rotor. In order to keep the core complexes in high activity, the whole isolation procedure was performed in the presence of glycine betain and pH at 6.3. The isolated pigment‐protein complexes were characterized by sodium dodecyl sulfate‐polyacrylamide gel electrophoresis, absorption spectroscopy, 77 K fluorescence spectroscopy and high performance liquid chromatography. Our results show that this method is a better choice for quick and efficient isolation of functionally active PSII core complexes.
H2 reformation in post-shock regions
Authors: Cuppen H. M. , Kristensen L. E. , Gavardi E. 
Published: May 2010

Abstract
H2 formation is an important process in post-shock regions, since H2 is an active participant in the cooling and shielding of the environment. The onset of H2 formation therefore has a strong effect on the temperature and chemical evolution in the post-shock regions. We recently developed a model for H2 formation on a graphite surface in warm conditions. The graphite surface acts as a model system for grains containing large areas of polycyclic aromatic hydrocarbon structures. Here, this model is used to obtain a new description of the H2 formation rate as a function of gas temperature that can be implemented in molecular shock models. The H2 formation rate is substantially higher at high gas temperatures as compared to the original implementation of this rate in shock models, because of the introduction of H atoms which are chemically bonded to the grain (chemisorption). Since H2 plays such a key role in the cooling, the increased rate is found to have a substantial effect on the predicted line fluxes of an important coolant in dissociative shocks [Oi] at 63.2 and 145.5 μm. With the new model, a better agreement between the model and observations is obtained. Since one of the goals of Herschel/PACS will be to observe these lines with higher spatial resolution and sensitivity than the former observations by Infrared Space Observatory-LWS, this more accurate model is very timely to help with the interpretation of these future results.