bluuebunny/crossref_metadata_2025_split huggingface dataset:
Requirement of MMP‐3 in anchorage‐independent growth of oral squamous cell carcinomas
Authors: Liu Shyun‐Yeu , Liu Young‐Chau , Huang Wen‐Tsung , Huang Guan‐Cheng , Su Hsiao‐Jing , Lin Mei‐Huei 
Published: August 2010

Background:  Matrix metalloproteinase‐3 (MMP‐3) is expressed in various carcinomas; however, its function is not clearly established. This study was to assess its possible role in oral squamous cell carcinomas (OSCCs).Materials and methods:  Specimens of seven oral pre‐malignant lesions (OPMLs) and 92 OSCCs were subjected to MMP‐3 detection by RT‐PCR and Western blot. Antisense oligodeoxynucleotides (AODNs) of MMP‐3 were used to transfect OSCC (OECM‐1 and SCC‐9) and esophageal carcinoma (CE81T/VGH) cell lines, and their growth was subsequently analyzed by XTT and soft‐agar colony assay.Results:  MMP‐3 transcript was preferentially expressed in OSCCs (71 of 92, 77%) than in OPMLs (two of seven, 29%; P = 0.012). Both MMP‐3 transcript and protein levels were significantly higher in OSCC masses than in neighboring tissues (P < 0.0001 and P = 0.04, respectively). Growth of the three cell lines was not affected, while the colony numbers of OECM‐1 and CE81T/VGH were significantly reduced by the transfection of MMP‐3 AODNs (P = 0.002 and P = 0.004, respectively). SCC‐9 did not form colonies in soft‐agar/medium.Conclusions:  MMP‐3 function may be required in most OSCCs, and it may support the anchorage‐independent growth of both OSCC and esophageal carcinoma.
Clinico‐pathologic correlations of myofibroblastic tumors of the oral cavity. II. Myofibroma and myofibromatosis of the oral soft tissues
Authors: Vered Marilena , Allon Irit , Buchner Amos , Dayan Dan 
Published: August 2010

Background:  Myofibroma is a solitary benign tumor of myofibroblasts. Myofibromatosis describes multiple, simultaneous myofibromas at different sites in various organs. The clinico‐pathologic correlations of myofibroma/myofibromatosis confined only to oral soft tissues were analyzed.Methods:  In the English language literature, 41 myofibroma and 12 myofibromatosis cases involving the oral soft tissues were found. From our files, three new myofibroma cases were added.Results:  Age at time of diagnosis of oral mucosa myofibroma ranged from birth to 70 years (mean 21.7 years), considerably higher than myofibroma in other parts of the body. Lesions occurred during the first decade (44%) and in the first year of life (17%). Male:female ratio was 1:1.6, contrary to the male predominance in other parts of the body. Common sites were the tongue (32%) and buccal mucosa (18%). Treatment was local excision, either complete (n = 13) or partial (n = 3), wide excision (n = 4), surgery, and chemotherapy (n = 1). Myofibromatosis involving oral soft tissues was diagnosed at birth in nine (75%) patients, within the first year in two, and as a young adult in one. Male:female ratio was 2:1. The tongue was the most common site (50%). Half the patients died of disseminated disease within a few days from birth, three were cured by partial or complete excision, and three experienced spontaneous regression. Histologically, oral mucosa myofibroma/myofibromatosis appearance agreed with findings in the literature.Conclusions:  Myofibroma should be included in the clinical differential diagnosis of masses of the oral soft tissues, especially in the tongue and buccal mucosa of children and adolescents. Histological differential diagnosis includes benign and malignant spindle‐shaped lesions. Treatment of choice is local excision.
Fcγ receptors mediate internalization of anti‐Ro and anti‐La autoantibodies from Sjögren’s syndrome and apoptosis in human salivary gland cell line A‐253
Authors: Lisi Sabrina , Sisto Margherita , Soleti Raffaella , Saponaro Concetta , Scagliusi Pasquale , D’Amore Massimo , Saccia Matteo , Maffione Angela B. , Mitolo Vincenzo 
Published: August 2010

Background:  The presence of serum anti‐Ro and anti‐La autoantibodies directed against the ribonucleoproteins Ro and La has been associated with Sjögren’s syndrome (SS), an autoimmune rheumatic disease that targets salivary and lachrymal glands. There is increasing evidence of the direct involvement of autoantibodies in the pathogenesis of tissue injury and correlation of their presence with clinical manifestations in SS. The focus of this work was to explore the cellular apoptotic pathway triggered by binding and penetration of anti‐Ro and anti‐La autoantibodies in human salivary gland cell line A‐253 and to identify the membrane receptors through which anti‐Ro and anti‐La could exert their effect.Methods:  Anti‐Ro and anti‐La autoantibodies were purified from IgG fractions, obtained from eleven healthy volunteers and patients with primary Sjögren’s syndrome, using Sepharose 4B‐Ro and Sepharose 4B‐La affinity columns. Flow cytometry, RT‐PCR, western blot and confocal microscopy analysis were used to visualize the FCγRI, FCγRII and FCγRIII receptors on the A‐253 cell membrane. DNA laddering and western blot analysis of caspases activation were studied to evaluate in A‐253 cells treated with anti‐Ro and anti‐La autoantibodies.Results:  The results yeilded the evidence of the presence of members of the Fcγ receptors (FcγRs) family on the cell membrane of the human salivary gland cell line A‐253. Furthermore, we demonstrated that, in the A‐253 cell line, anti‐Ro and anti‐La autoantibodies can access the cells probably through Fcγ receptors, and trigger apoptotis.Conclusions:  We conclude that anti‐Ro and anti‐La autoantibodies have pathogenic effects that could depend on binding to Fcγ receptors.
Immunolocalization of cell signaling molecules in the granular cell ameloblastoma
Authors: Sathi Gul San Ara , Han Phuu Pwint , Tamamura Ryo , Nagatsuka Hitoshi , Hu Hailong , Katase Naoki , Nagai Noriyuki 
Published: August 2010

Background:  Bone morphogenic protein (BMP) and Wnt signaling pathway molecules play important roles in cytodifferentiation and cell proliferation. We attempted to localize these signaling molecules in the granular cell ameloblastoma.Materials and methods:  Four samples of paraffin‐embedded ameloblastoma with granular cells were studied. Immunohistochemistry was performed to detect basement membrane type heparan sulfate (HS) (JM403), cell surface type HS (10E4), heparanase, Wnt‐5a, Wnt‐2, β‐catenin, and BMP‐4.Results:  In all four samples, strong expression of β‐catenin and Wnt‐5a was detected within the granular cells, while BMP‐4 expression was weak and Wnt‐2 was negative. Immunoreactivities of basement membrane type HS, cell surface type HS, and heparanase were variable within granular cells in ameloblastoma.Conclusion:  Granular cells in ameloblastoma exhibit abnormal biological behaviors, particularly synthesis and secretion of protein. Synthesis of signaling molecules is upregulated, but secretion is arrested in some cases, while both are lost in other cases.
ORIGINAL ARTICLE: Circulating Antibodies to a Conserved Epitope of the Chlamydia Trachomatis 60‐kDa Heat Shock Protein is Associated with Decreased Spontaneous Fertility Rate in Ectopic Pregnant Women Treated by Salpingectomy
Authors: Sziller István , Fedorcsák Peter , Csapó Zsolt , Szirmai Katalin , Linhares Iara M , Papp Zoltàn , Witkin Steven S 
Published: August 2010

Problem  This prospective study was aimed to evaluate whether non‐invasive clinical and serologic parameters of tubal disease are predictive for subsequent spontaneous conception and pregnancy outcome after first episode of ectopic pregnancy (EP).Method of study  Overall, 144 women aged <35 years were enrolled. Outcome of subsequent spontaneous conception was analyzed after 3 years and compared with clinical parameters and antibody responses to Chlamydia trachomatis and epitopes of the 60‐kDa chlamydial heat shock protein (CHSP‐60).Results  Antibody response to a conserved epitope of CHSP‐60 (amino acids, aa 260–277) was independently correlated with both decreased spontaneous conception and term delivery rates (adjusted odds ratios, OR: 3.6 and 5.4, respectively).Conclusion  Presence of circulating antibodies to a conserved epitope of the CHSP‐60 is associated with a lower spontaneous conception rate, and increased likelihood of adverse pregnancy outcome in women treated by salpingectomy for first episode of EP.
Early prediction of neurological sequelae or death after bacterial meningitis
Authors: Oostenbrink R , Moons KGM , Derksen‐Lubsen G , Grobbee DE , Moll HA 
Published: August 2010

This study determined independent predictors of the occurrence of permanent neurological sequelae or death after childhood bacterial meningitis. Data were used from a large study on children (aged 1 mo to 15 y) initially presenting with meningeal irritation. A nested case‐control study was performed on children with (n= 23) and without (n= 70) permanent neurological sequelae (hearing impairment, locomotor dysfunction, mental retardation or epilepsy) or death after bacterial meningitis. Predictors obtained from clinical evaluation and laboratory tests at presentation and during the clinical course were identified by multivariate logistic regression and receiver operating characteristic (ROC) curve analyses. The study population comprised 23 cases and 70 controls (52% boys, median age 2.8 y). Independent predictors for an adverse outcome after bacterial meningitis were male gender, atypical convulsions in history, low body temperature at admission and the pathogen Streptococcus pneumoniae. The area under the ROC curve of this prediction rule was 0.87 (95% confidence interval: 0.78–0.96), which was not improved by adding other characteristics. A score including these independent predictors could classify patients into categories with increasing risk for an adverse outcome. Conclusion: Clinical characteristics available early in the clinical course, such as gender, atypical convulsions in history, low body temperature at admission and the pathogen, are predictive for the occurrence of permanent neurological sequelae or death after bacterial meningitis in childhood. The pathogen type, in particular, is the main prognostic determinant of childhood bacterial meningitis.
Size at birth and neonatal and postneonatal mortality
Authors: Cheung YB , Yip PSF , Karlberg JPE 
Published: August 2010

Birthweight is a crude indicator of size at birth. Some neonatologists and obstetricians have advocated the use of ponderal index and birthlength to characterize size at birth. This paper examines the associations between various size‐at‐birth indicators and neonatal and postneonatal mortality, with an emphasis on ponderal index and birthlength. Size at birth, gestational age and mortality data for about one million babies born alive in Sweden between 1987 and 1995 were collected from the Swedish Medical Birth Registry. A multinomial logit regression was used to estimate conditional odds ratios. Birthlength and ponderal index were independently associated with neonatal and postneonatal mortality. In the latter period, ponderal index was only weakly associated with mortality. The associations were not sensitive to exclusion of cases of congenital anomalies and adjustment for gestational age. Conclusion: Birthlength is strongly associated with both neonatal and postneonatal mortality; ponderal index is strongly associated with neonatal, but weakly with postneonatal mortality. The findings are consistent with previous hypotheses about a transient effect of ponderal index and a persistent effect of birthlength.
A clinical study of 77 patients with mucopolysaccharidosis type II
Authors: Schwartz Ida VD , Ribeiro Márcia G , Mota João G , Toralles Maria Betânia P , Correia Patrícia , Horovitz Dafne , Santos Emerson S , Monlleo Isabella L , Fett‐Conte Agnes C , Sobrinho Ruy P Oliveira , Norato Denise YJ , Paula Anna Carolina , Kim Chong A , Duarte Andréa R , Boy Raquel , Valadares Eugênia , De Michelena Maria , Mabe Paulina , Martinhago Cyro D , Pina‐Neto João M , Kok Fernando , Leistner‐Segal Sandra , Burin Maira G , Giugliani Roberto 
Published: August 2010

AbstractAim: This study aims to assess the clinical features of 77 South American patients (73 Brazilian) with mucopolysaccharidosis type II (MPS II).Methods: Details of the patients and their disease manifestations were obtained from a review of medical records, interviews with the patients and/or their families, and physical examination of the patients.Results: Mean birth weight was 3360 g, median age at onset of symptoms was 18 months and median age at diagnosis was 6 years. For the whole sample (median age, 8.2 years; range, 2.8–53.0 years), neurological degeneration, typical pebbly skin lesions, seizures and extensive dermal melanocytosis were found in 23.3, 13.0, 13.0 and 1.3% of the cases, respectively. The most frequently reported echocardiogram abnormality was mitral valve regurgitation. Refraction errors were the most common ophthalmological manifestation. The following characteristics were found to be associated with the severe form of MPS II: earlier age at biochemical diagnosis, higher levels of urinary glycosaminoglycans, language development delay, behavioural disturbances, poor school performance and mental retardation.Conclusion: Our results suggest that there is a considerable delay between the onset of signs and symptoms and the diagnosis of MPS II in Brazil (and probably in South America as well), and that many complications of this disease are underdiagnosed and undertreated. Therefore, the implementation of programmes aiming to increase the awareness of the disease, the availability of biochemical diagnostic tests and the provision of better support to affected patients is urgently needed.
Gastrointestinal symptoms in Fabry disease: everything is possible, including treatment
Authors: Hoffmann Björn , Keshav Satish 
Published: August 2010

AbstractGastrointestinal symptoms in Fabry disease were first described independently by William Anderson and Johannes Fabry. Case reports and case series suggest that the whole gastrointestinal tract may be affected in patients with Fabry disease. The Fabry Outcome Survey (FOS) database supports these observations. The overall prevalence of gastrointestinal involvement reported in a study of 342 patients (271 adults; 71 children) enrolled in FOS was 52% (49.8% in adults, 60.8% in children). Abdominal pain was the most prevalent symptom. The median age at onset of gastrointestinal symptoms was 14 years, similar to the age at onset of acroparaesthesia. The prevalence of gastrointestinal symptoms was reduced in patients after receiving enzyme replacement therapy with agalsidase alfa for 12 and 24 months. There was no correlation between gastrointestinal symptoms and body mass index.Conclusion: Gastrointestinal symptoms in Fabry disease may have been underestimated. The FOS database supports previous reports of beneficial effects of enzyme replacement therapy on gastrointestinal symptoms in Fabry disease.
Training of attention and memory deficits in children with acquired brain injury
Authors: Sjö N Madsen , Spellerberg S , Weidner S , Kihlgren M 
Published: August 2010

AbstractThis pilot study concerns cognitive rehabilitation of children with acquired brain injury (ABI).Aim:  The aim is threefold; to determine (1) whether the Amsterdam Memory and Attention Training for Children (AMAT‐C) programme for children with ABI can be integrated in the child’s school, (2) whether supervision in the school‐setting maintains the child’s motivation throughout the training programme and (3) whether positive changes in memory, attention and executive functions are found with this implementation of the training method.Methods:  Seven children with memory and/or attention deficits after ABI were trained with AMAT‐C. Measures used were programme evaluation questions, neuropsychological tests and a questionnaire concerning executive functions.Results:  Overall, children, parents and trainers were satisfied with the programme and the children were motivated throughout the programme. The children showed significant improvements in neuropsychological subtests, primarily in tests of learning and memory. No overall change in executive functions was noted.Conclusion:  Provision of AMAT‐C training and supervision at the child’s school appears to ensure (1) satisfaction with the programme, (2) sustaining of motivation and (3) improvements in learning and memory.
Evaluation of Quality of Life and Sexual Satisfaction in Women Suffering from Chronic Pelvic Pain With or Without Endometriosis
Authors: Tripoli Tatiana M. , Sato Hélio , Sartori Marair G. , de Araujo Fábio Fernando , Girão Manoel J.B.C. , Schor Eduardo 
Published: August 2010

ABSTRACT

Introduction
Chronic pelvic pain (CPP) is one of the most frequent symptoms in women of reproductive age. This is an enigmatic clinical condition that results from the complex interactions of physiological and psychological factors with direct impact on the social, marital, and professional lives of women.

Aim
To evaluate the quality of life and sexual satisfaction of women who suffer from CPP with or without endometriosis.

Method
Forty-nine patients who had been diagnosed with endometriosis and 35 patients with CPP diagnosed with another gynecological condition, all 84 of whom were treated at the Chronic Pelvic Pain and Endometriosis Clinic at Universidade Federal de São Paulo (UNIFESP) from January to July of 2008. The controls were 50 healthy women from the Family Planning Clinic at UNIFESP.

Main Outcome Measures
World Health Organization Quality of Life Assessment-Bref (WHOQOL-BREF) quality of life questionnaire and the Golombok-Rust Inventory of Sexual Satisfaction (GRISS).

Results
No statistically significant differences were observed between the groups with CPP symptoms, in either the results from the WHOQOL-BREF or in the GRISS questionnaire. In both questionnaires, differences were observed when the two groups of symptomatic women were compared with the group of healthy women.

Conclusion
CPP caused by endometriosis or other gynecological conditions leads to a significant reduction of quality of life and sexual satisfaction.
Prostate-Specific Antigen Levels are Associated with Arterial Stiffness in Essential Hypertensive Patients
Authors: Vyssoulis Gregory , Karpanou Eva , Kyvelou Stella-Maria , Vlachopoulos Charalambos , Tzamou Vanessa , Stefanadis Christodoulos 
Published: August 2010

ABSTRACT

Introduction
Prostate-specific antigen (PSA) has been recently related to cardiovascular system in a multifactorial way. Arterial stiffness is a independent predictor of cardiovascular events and is involved in the pathogenesis of hypertension. The aim of the present study was to investigate whether PSA values, are associated with arterial stiffness indices in patients with essential arterial hypertension.

Methods
The study comprised 150 consecutive male patients (mean age 60 years) with uncomplicated never-treated essential hypertension. All patients underwent a complete clinical and laboratory evaluation, including measurement of PSA levels. Aortic stiffness and arterial wave reflection assessment was made by using carotid-femoral (PWVc-f) pulse wave velocity and aortic augmentation index corrected for heart rate (AIx75). Patients with prostate cancer or benign prostate hyperplasia (PSA &gt; 4 ng/mL) were excluded from the study.

Results
PSA was positively associated with waist-to-hip ratio (r = 0.235, P = 0.04), PWVc-f (r = 0.426, P &lt; 0.001), AIx75 (r = 0.264, P = 0.001), and high sensitivity C-reactive protein (hsCRP; r = 0.376, P &lt; 0.001). In categorization to PSA quartiles, patients in the higher quartile presented with higher waist-to hip ratio (P = 0.009), PWVc-f (P &lt; 0.00001), AIx75 (P &lt; 0.001) and hsCRP (P &lt; 0.001) values. In multivariate analysis after adjustment for various confounders PSA remained a significant determinant of PWVc-f values (beta [SE] = 0.477 [0.13], R2 = 0.405, P &lt; 0.001).

Conclusion
The present study points towards an association between PSA levels and aortic stiffness in untreated essential hypertensive males. Potential causal relationships between PSA and arterial stiffness remain to be further explored.
Sexual Function in Obese Women with and without Binge Eating Disorder
Authors: Castellini Giovanni , Mannucci Edoardo , Mazzei Chiara , Lo Sauro Carolina , Faravelli Carlo , Rotella Carlo M. , Maggi Mario , Ricca Valdo 
Published: August 2010

ABSTRACT

Introduction
Binge eating disorder (BED) is highly prevalent among individuals seeking treatment for obesity. No controlled studies assessing the sexual functioning of these patients have been published so far.

Aim
To investigate the sexual functioning of a clinical sample of obese women affected by BED, comparing them with obese non-BED patients (Ob), and with normal weight controls.

Methods
A consecutive series of 107 obese BED and 110 obese non-BED patients referring for the first time to the Clinic for Obesity of the University of Florence, together with a control group of 92 normal weight subjects, were studied.

Main Outcome Measures
Patients were studied by means of the Structured Clinical Interview for DSM-IV and the Female Sexual Function Index (FSFI). Moreover, several self-reported questionnaires assessing the eating specific and general psychopathology were used.

Results
BED and obese non-BED probands reported a lower sexual activity compared to controls, in terms of absence of sexual intercourse rate, and sexual intercourse frequency. BED patients showed lower FSFI total and subscales scores compared to Ob, and Ob probands reported lower scores compared to controls. According to the multiple linear regression analysis, emotional eating was the main determinant of FSFI scores (FSFI total score, desire, arousal, lubrication, orgasm, satisfaction) for both BED and Ob patients, while impulsivity (inversely associated with FSFI total, orgasm, and pain) and shape concern (inversely associated with arousal, lubrication, orgasm) were main determinants for BED patients only.

Conclusions
BED patients, compared to obese non-BED and controls, have worse sexual functioning, which is associated with high levels of emotional eating, impulsivity, and shape concerns. The relationship between sexual functioning and eating psychopathology should be carefully addressed in obese patients with and without BED.
A new allele, HLA‐B*4212, identified in a Brazilian volunteer bone marrow donors by sequence‐based typing
Authors: Mattar S. B. , Uehara C. J. S. , Ribas F. , Vargas R. G. , Wowk P. F. , Poerner F. , Bicalho M. G. 
Published: August 2010

SummaryHere we report the discovery of a novel HLA‐B allele, named B*4212 in a Brazilian volunteer bone marrow donor. The new sequence has nucleotide variation at position 496 (T→G) as compared with B*4201. This variation results in a conservative amino acid substitution from valine to glycine at codon 165 of exon 3.