bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid protein production using CHO stable transfection pools
Authors: Ye Jianxin , Alvin Krista , Latif Haythem , Hsu Amy , Parikh Vanessa , Whitmer Travis , Tellers Melanie , de la Cruz Edmonds M. Celina , Ly Jeffrey , Salmon Peter , Markusen Julia F. 
Published: June 2010

AbstractDuring early preclinical development of therapeutic proteins, representative materials are often required for process development, such as for pharmacokinetic/pharmacodynamic studies in animals, formulation design, and analytical assay development. To rapidly generate large amounts of representative materials, transient transfection is commonly used. Because of the typical low yields with transient transfection, especially in CHO cells, here we describe an alternative strategy using stable transfection pool technology. Using stable transfection pools, gram quantities of monoclonal antibody (Mab) can be generated within 2 months post‐transfection. Expression levels for monoclonal antibodies can be achieved ranging from 100 mg/L to over 1000 mg/L. This methodology was successfully scaled up to a 200 L scale using disposable bioreactor technology for ease of rapid implementation. When fluorescence‐activated cell sorting was implemented to enrich the transfection pools for high producers, the productivity could be improved by about three‐fold. We also found that an optimal production time window exists to achieve the highest yield because the transfection pools were not stable and productivity generally decreased over length in culture. The introduction of Universal chromatin‐opening elements elements into the expression vectors led to significant productivity improvement. The glycan distribution of the Mab product generated from the stable transfection pools was comparable to that from the clonal stable cell lines. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Towards the implementation of quality by design to the production of therapeutic monoclonal antibodies with desired glycosylation patterns
Authors: del Val Ioscani Jimenez , Kontoravdi Cleo , Nagy Judit M. 
Published: June 2010

AbstractQuality by design (QbD) is a scheme for the development, manufacture, and approval of pharmaceutical products. The end goal of QbD is to ensure product quality by building it into the manufacturing process. The main regulatory bodies are encouraging its implementation to the manufacture of all new pharmaceuticals including biological products. Monoclonal antibodies (mAbs) are currently the leading products of the biopharmaceutical industry. It has been widely reported that glycosylation directly influences the therapeutic mechanisms by which mAbs function in vivo. In addition, glycosylation has been identified as one of the main sources of monoclonal antibody heterogeneity, and thus, a critical parameter to follow during mAb manufacture. This article reviews the research on glycosylation of mAbs over the past 2 decades under the QbD scope. The categories presented under this scope are: (a) definition of the desired clinical effects of mAbs, (b) definition of the glycosylation‐associated critical quality attributes (glycCQAs) of mAbs, (c) assessment of process parameters that pose a risk for mAb glycCQAs, and (d) methods for accurately quantifying glycCQAs of mAbs. The information available in all four areas leads us to conclude that implementation of QbD to the manufacture of mAbs with specific glycosylation patterns will be a reality in the near future. We also foresee that the implementation of QbD will lead to the development of more robust and efficient manufacturing processes and to a new generation of mAbs with increased clinical efficacy. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Management of residual shunts after initial percutaneous patent foramen ovale closure: A single center experience with immediate and long‐term follow‐up
Authors: Diaz Tulio , Cubeddu Roberto J. , Rengifo‐Moreno Pablo A. , Cruz‐Gonzalez Ignacio , Solis‐Martin Jorge , Buonanno Ferdinando S. , Inglessis Ignacio , Palacios Igor F. 
Published: June 2010

AbstractBackground: Moderate‐to‐large residual shunts following percutaneous patent foramen ovale (PFO) closure are clinically important and associated with recurrent neuroembolic events. However, their management has not been clearly established in clinical practice. We report our experience in patients of these patients with a prior history of cryptogenic stroke and/or transient ischemic attack (TIA). Methods: All patients undergoing percutaneous PFO closure were routinely screened at six‐months for residual shunts using transthoracic 2D echocardiography with antecubital administration of agitated saline contrast and color flow Doppler. Patients with evidence of moderate‐to‐large residual shunts were selected to undergo reintervention with shunt closure. Postreintervention follow‐up was performed at 24‐hr, 30 days, and every six months thereafter. Clinical predictors of the moderate‐to‐large residual shunts, and the feasibility, safety and long‐term efficacy of percutaneous residual shunt closure using a second device implant were examined. Results: Between 1995 and 2007, a total of 424 patients underwent PFO closure. Of these, 5% (21/424) had moderate‐to‐large residual shunts. Baseline characteristics among patients with moderate‐to‐large residual shunts and those with only none or small defects (n = 403) were similar. Multivariate analysis identified the 24‐hr postprocedure shunt as the only independent predictor of residual shunting at six months. Of the 21 study patients with moderate‐to‐large residual shunt (mean age, 47 ± 14), one underwent successful elective surgical repair, while the remaining 20 underwent transcatheter closure using a second device. The technique was successful in 95% (19/20), and all but one patient had complete shunt closure at six months of their percutaneous reintervention. We report no deaths, recurrent strokes or TIAs during the long‐term mean follow‐up period of 2.9 years. Conclusions: Our study suggests that in patients with moderate‐to‐large residual PFO shunts, percutaneous reintervention using a second device implant is safe and effective. © 2010 Wiley‐Liss, Inc.
Complex transcatheter paravalvular leak repair
Authors: Jilaihawi Hasan , Ibrahim Réda 
Published: June 2010

AbstractThe technical challenges and feasibility of complex paravalvular leak repair are discussed through a case based example. A frail septogenarian presented with dyspnea due to severe paravalvular mitral regurgitation. She had severe scoliosis and cardiac cachexia. There were problems with hemolysis necessitating repeated blood transfusion. Logistic EuroSCORE was 36.4% and STS score 15.5% for mortality, 53.2% morbidity or mortality. NYHA class was IV with progressive symptoms necessitating prolonged hospitalization. The surgical opinion was that redo surgery would confer excessive risk and she was declined for further open surgery. Amplatzer transcatheter paravalvular leak repair was performed using multiple devices. Predischarge there was a marked improvement in symptoms, from NYHA IV (immobile and bedbound) to NYHA II. The patient was discharged improved but remained frail. She continued to have problems with hemolysis, although the frequency of blood transfusions required declined significantly. At 3 months, the LV cavity dimension decreased from 47 mm to 38 mm, still with preserved LV systolic function. There was trace paravalvular MR, which was sustained at 6 months. Complex paravalvular leaks can be repaired with Amplatzer devices by transcatheter approach in very high risk patients. Several tools may be required to overcome anatomical challenges. A partially successful or unsuccessful initial procedure may yield an excellent final result through judicious staging and perseverance. © 2010 Wiley‐Liss, Inc.
Complete versus culprit only revascularization in acute ST elevation myocardial infarction: A meta‐analysis
Authors: Sethi Ankur , Bahekar Amol , Bhuriya Rohit , Singh Sarabjeet , Ahmed Aziz , Khosla Sandeep 
Published: June 2010

AbstractBackground: Current guidelines recommend against the revascularization of noninfarct related artery (complete revascularization [CR]) in patients with ST elevation myocardial infarction (STEMI) and no hemodynamic compromise, though level of evidence is C. Aim: Our aim was to examine the available evidence to determine any advantage of CR over culprit only revascularization (COR). Methods: We systematically searched medline using key words—“culprit coronary revascularization,” “complete revascularization myocardial infarction,” and “multivessel STEMI” for studies reporting outcomes after COR versus CR during primary procedure or index hospitalization published in English language and indexed before February 2010. A random effect or fixed effect meta‐analysis, as applicable, was performed using RevMan 5 (Cochrane Center, Denmark). Results: Nine eligible nonrandomized studies amounting to 4,530 patients in CR and 27,323 patients in COR group were included. In addition, two small randomized trials were reviewed and included in secondary analysis. Majority of patients were hemodynamically stable. Major adverse cardiovascular events (Odds ratio [OR] = 0.95, 95% CI 0.47–1.90) and long term mortality (OR = 1.10, 95% CI 0.76–1.59) were similar. The marginal increased odds of in‐hospital mortality was derived from a single study with no difference found after sensitivity and cumulative analysis (OR = 1.21 95% CI 0.85–1.73). Conclusion: Current analysis of heterogeneous studies did not reveal any benefit of CR over COR in patients with STEMI. However, also provide no conclusive evidence of increased in hospital mortality after CR. A randomized trial is needed to confirm these findings and recognize any subgroup which might benefit from CR. © 2010 Wiley‐Liss, Inc.
Transcatheter closure of a patent ductus arteriosus in a preterm infant with an Amplatzer Vascular Plug IV device
Authors: Prsa Milan , Ewert Peter 
Published: June 2010

AbstractRapid advances in paediatric interventional cardiology allow transcatheter closure of patent ductus arteriosus (PDA) in increasingly smaller patients. We describe a successful transcatheter closure of a large tubular PDA in a symptomatic preterm infant of 2,210 g using an Amplatzer Vascular Plug IV device. © 2010 Wiley‐Liss, Inc.
Angiographic patency and clinical outcome after balloon‐angioplasty for extensive infrapopliteal arterial disease
Authors: Schmidt Andrej , Ulrich Matthias , Winkler Bert , Klaeffling Christina , Bausback Yvonne , Bräunlich Sven , Botsios Spiridon , Kruse Hans‐Joachim , Varcoe Ramon L. , Kum Steven , Scheinert Dierk 
Published: June 2010

AbstractBackground and objective: Restenosis‐rate after balloon‐angioplasty of long segment tibial arterial disease is largely unknown. We investigated the restenosis‐rates angiographically in patients with critical limb ischemia (CLI) due to extensive infrapopliteal lesions. Methods: Angioplasty for infrapopliteal lesions exclusively ≥80 mm in length was performed using dedicated 80–120 mm long low‐profile balloons. Follow‐up included angiography at 3 months and clinical assessment at 3 and 15 months. Results: Angioplasty was performed in 77 infrapopliteal arteries of 62 limbs of 58 CLI patients with a Rutherford class 4 in 16 (25.8%) limbs and Rutherford class 5 in 46 limbs (74.2%). Average lesion length was 18.4 cm. Treated arteries were stenosed in 35.1% and occluded in 64.9%. After 3 months, a clinical improvement (marked reduction of ulcer‐size or restpain) was seen in 47 (75.8%) limbs, 14 (22.6%) limbs were clinically unchanged and 1 (1.6%) limb showed a clinical deterioration. Angiography at 3 months showed no significant restenosis in 24 of 77 (31.2%) treated arteries, a restenosis ≥50% in 24 (31.2%) arteries and a reocclusion in 29 of 77 (37.6%). At 15 months death rate was 10.5%. After repeat angioplasty in case of restenosis cumulative clinical results at 15 months were minor amputations in 8.1%, no major amputations resulting in a limb‐salvage rate of 100% with no patient requiring bypass surgery. Conclusions: Restenosis‐rate after angioplasty of extensive infrapopliteal arterial disease is high and occurs early after treatment. Despite this the clinical results are excellent, especially given the length of the arterial segments diseased. © 2010 Wiley‐Liss, Inc.
Transcatheter interventions in the early postoperative period after the Fontan procedure
Authors: Bhole Vinay , Wright John G.C. , De Giovanni Joseph V. , Dhillon Rami , Miller Paul A. , Desai Tarak , Chikermane Ashish , Jones Timothy , Barron David J. , Brawn William J. , Stumper Oliver 
Published: June 2010

AbstractObjective: To analyze the safety and clinical impact of interventional cardiac catheter procedures in the management of early postoperative problems after completion of an extracardiac Fontan procedure. Background: The mortality after Fontan procedure has consistently decreased over the last decade. The role of interventional catheterization to address early postoperative problems in this setting has not been studied systematically. Methods: Over a 9.7‐year period, 289 patients underwent an extracardiac fenestrated Fontan procedure with two early deaths (0.7%) and takedown in four (1.4%). Twenty‐seven patients (9.3%) underwent 32 interventional cardiac catheter procedures at a median interval of 12.2 (1–30) days. The median weight was 14.5 (13.5–25) kg. The case notes and procedure records were reviewed retrospectively. Results: Fontan pathway obstructions were treated in 11 patients with stent implantation with good results and no complications. Stent fenestration of the Fontan circulation was performed in 16 patients with one episode of transient hemiparesis and one episode of pericardial effusion. Three patients underwent initial balloon dilatation of branch pulmonary arteries or fenestration with little effect and underwent stent treatment 6 (5–9) days later. One patient had device closure of a large atrial fenestration. In one patient, residual anterograde pulmonary blood flow was occluded using a device. There were no deaths and in‐hospital course was improved in all. Conclusion: Interventional cardiac catheter procedures can be performed safely and effectively in the early postoperative period after Fontan completion to address hemodynamic problems. These techniques contribute significantly to achieve a very low mortality and address morbidity after Fontan completion. © 2010 Wiley‐Liss, Inc.
Homogeneous Stabilization of Pt Nanoparticles in Dendritic Core–Multishell Architectures: Application in Catalytic Hydrogenation Reactions and Recycling
Authors: Keilitz Juliane , Schwarze Michael , Nowag Sabrina , Schomäcker Reinhard , Haag Rainer 
Published: June 2010

AbstractCore–multishell architectures are a new approach to homogeneously stabilize metal nanoparticles for harsh conditions. Herein, we present the synthesis and stabilization of Pt nanoparticles in dendritic core–multishell polymers and their application in hydrogenation reactions. The successful recycling of the catalyst was demonstrated for the hydrogenation of methyl crotonate 1 and was either achieved by ultrafiltration or in a two‐phase system for at least 14 cycles. Thereby, the total turnover number (TON) was increased to 22 000. In the recycling experiments, low metal leaching into the product (as low as 0.3 ppm) was detected. Additionally, the selective hydrogenation of isophorone 3 was investigated and selectivities of 99:1 for CC versus CO hydrogenation were achieved.
Nonlinear Predictive Control of Fed‐Batch Cultures of Escherichia coli
Authors: Tebbani S. , Dumur D. , Hafidi G. , Vande Wouwer A. 
Published: June 2010

AbstractA strategy for controlling a fed‐batch Escherichia coli culture is described to maintain the culture at the boundary between oxidative and oxido‐fermentative regimes. A nonlinear predictive controller is designed to regulate the acetate concentration, constraining the feed rate to follow an optimal reference profile which maximizes the biomass growth. For the sake of simplicity and efficiency, the original problem is converted into an unconstrained nonlinear programming problem, solved by control vector parameterization techniques. The robustness of the structure is further improved by explicitly including the difference between system and model prediction. A robustness study based on a Monte Carlo approach is used to evaluate the performance of the proposed controller. This control law is finally compared to the generic model control strategy.
Heat Pipe‐Cooled Microstructured Reactor Concept for Highly Exothermal Ionic Liquid Syntheses
Authors: Löwe H. , Axinte R. D. , Breuch D. , Hang T. , Hofmann C. 
Published: June 2010

AbstractHeat pipes used for cooling of microstructured reactors are a new approach for sustainable processing also in the lab‐scale within a temperature range from ambient to more than 180 °C. The main advantage of heat pipe cooling is the dynamic behavior, i.e., the cooling rate depends on the heat released. Heat pipes can also suppress thermal runaways due to their extremely short response times on momentary temperature rises. As an example, the highly exothermal synthesis of 1‐ethyl‐3‐methylimidazolium trifluoromethanesulfonate from the respective reactants 1‐ethyl‐imidazole and methyltrifluoromethanesulfonate was investigated. By transferring the protocol to continuous‐flow conditions in the microscale and by applying cooling with heat pipes, an out‐of‐control processing can be avoided.
Coupled DEM‐CFD Simulation of Pneumatically Conveyed Granular Media
Authors: Sturm M. , Wirtz S. , Scherer V. , Denecke J. 
Published: June 2010

AbstractA DEM‐CFD coupling for the simulation of gas‐solid flows was successfully implemented and simulations were performed for the application to industrial‐scale pneumatic conveying. Therefore, all particle collisions and phase interactions were considered and porosity determination was optimized. The aim of this work is to show the applicability of the presented simulation model to the different regimes of pneumatic conveying systems. As a first test case a dense vertical pneumatic conveying system was chosen and an individual plug was investigated in detail. Variations of the conveying air velocity were also considered. As a second test case dilute conveying in a horizontal‐to‐vertical pipe bend was simulated. The occurrence of roping and the reduction of particle velocity is of high interest for the design of specific pneumatic systems. It is shown that both regimes can be captured reasonably well and the results are rich in details.
Synthesis of Small Molecules with High Scaffold Diversity: Exploitation of Metathesis Cascades in Combination with Inter‐ and Intramolecular Diels–Alder Reactions
Authors: O'Leary‐Steele Catherine , Pedersen Palle J. , James Thomas , Lanyon‐Hogg Thomas , Leach Stuart , Hayes Jerome , Nelson Adam 
Published: June 2010

AbstractOur knowledge of the biological relevance of regions of chemical space is shaped, in large part, by the synthetic accessibility of small molecules. Historically, however, chemists have explored chemical space in an exceptionally uneven and unsystematic way. We have previously demonstrated that metathesis cascade chemistry may be harnessed to yield small molecule collections with high scaffold diversity. Here, we describe the extent to which inter‐ and intramolecular Diels–Alder reactions, when used in conjunction with metathesis cascades, can extend the range of molecular scaffolds that are accessible. A range of metathesis substrates was prepared from combinations of two or three building blocks. Metathesis cascades were exploited to “reprogram” the molecular scaffolds. In many cases, the metathesis products were 1,3‐dienes, which were potential substrates for either inter‐ or intramolecular Diels–Alder reactions. The synthesis and functionalisation of the products was often facilitated by fluorous tagging, for example by using a “safety‐catch” linker that we have developed. It was demonstrated that, in certain cases, Diels–Alder reactions could extend the range of molecular scaffolds that may be prepared by using metathesis cascade reactions.
ChemInform Abstract: Gas‐Phase Molecular Structures of Third Row Transition‐Metal Hexafluorides WF6, ReF6, OsF6, IrF6, and PtF6. An Electron‐Diffraction and ab initio Study.
Authors: Richardson Alan D. , Hedberg Kenneth , Lucier George M. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: CsTiP2O7.
Authors: Huang Fu Qiang , Ibers James A. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Transport and Phase Transition Characteristics in AgI:Al2O3 Composite Electrolytes. Evidence for a Highly Conducting 7‐Layer AgI Polytype.
Authors: Lee Jong‐Sook , Adams Stefan , Maier Joachim 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Preparation and Dielectric Properties of Ceramics in the SrBi2(Ta1‐xNbx)2O9 Solid Solution System.
Authors: Chen X. M. , Hu G. L. , Yang J. S. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Electronic Structure of Ring and Chain Substituted Styrenes and Their Reactivities in the Reaction with Peroxyl Radicals.
Authors: Opeida Iosyp O. , Suprun Wolodymyr Ya. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Effect of Reaction Conditions on the Kinetic and Activation Parameters for the Mild Introduction of Fluorine into α‐Substituted Styrenes with Accufluor NFTh.
Authors: Stavber Stojan , Pecan Tjasa Sotler , Zupan Marko 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Chiral Hydroxyl Phospholanes from D‐Mannitol and Their Use in Asymmetric Catalytic Reactions.
Authors: Li Wenge , Zhang Zhaoguo , Xiao Dengming , Zhang Xumu 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.