bluuebunny/crossref_metadata_2025_split huggingface dataset:
TESTOSTERONE EXACERBATES THE LEFT VENTRICULAR HYPERTROPHY OF THE FEMALE OVARIECTOMIZED SPONTANEOUSLY HYPERTENSIVE RATS THROUGH INHIBITING THE ACTIVATION OF AMPK- FOXO1/MURF1 SIGNAL
Authors: Zhu Lulu , Wang Wengting , Chen Jianshu , Wang Qiongying , Yu Jing 
Published: May 2024

Objective:
Relative elevated testosterone concentrations induced left ventricular hypertrophy of the postmenopausal hypertension women, but the molecular mechanisms are poorly understood. MuRF1, a muscle-specific E3 ubiquitin ligase in the proteasome ubiquitination process, was newly identified as a negative regulatory factor of the heart and muscle tissue. There have been little study to certify whether MuRF1 take part in testosterone-induced the left ventricular hypertrophy of the postmenopausal hypertensive women.

Design and method:
Femal spontaneously hypertensive rats (SHR) (n = 28, 12week ages) were divided into three groups: (1) Sham group (n=6), (2) ovariectomized (OVX, n=6), (3) ovariectomized + testosterone intervention (OVX+T, 2mg/kg, QOD, n=6), in addition, the age-matched Wistar-Kyota (WKY) as the control. Testosterone was intramuscularly injected after ovariectomy and replaced every three days at 2mg/kg concentration for 4 weeks. we compared the weight/tibial length and the levels of IVST, LVPWT, LVM among four groups, and measured the expression of the myosin heavy chain (MHC), AMPK, FOXO1, MuRF1 and ubiquitinated proteins, as well as the phosphorylation expression of AMPK (p-AMPK) and Forkhead box O 1 (p-FOXO1) proteins by using Western Blot method. ANOVA analysis was used for comparison among the different groups. There was statistically significant difference when the p value was less than 0.05. The data were processed by SPSS 26.0 software.

Results:
After 4 weeks of testosterone intervention, compared to other three groups, the rats from OVX+T group showed the greatest elevation in blood pressure, the weight/tibial length and the levels of IVST, LVPWT, LVM and the protein expression of the MHC protein, but had a lowest expression of the p-AMPK, p-FOXO1, MuRF1 and ubiquitinated proteins.

Conclusions:
Collectively, Atrophy-related MuRF1 molecular might as an essential molecular mechanism modulated testosterone-induced the left ventricular hypertrophy of female ovariectomized SHR in AMPK- FOXO1-dependent signalling pathway manner.
PREGNANCY-RELATED SPONTANEOUS CORONARY ARTERY DISSECTION: CLINICAL PROFILE, PREGNANCY COMPLICATIONS AND OUTCOMES- SCAD-POL REGISTRY ANALYSIS
Authors: Zalewska Joanna , Michalowska Ilona , Gamski Maciej , Gziut-rudkowska Aneta , Wolny Rafal , Prejbisz Aleksander , Kadziela Jakub Jan , Januszewicz Andrzej , Witkowski Adam , Kadziela Jacek 
Published: May 2024

Objective:
Pregnancy associated spontaneous coronary artery dissection (P-SCAD) remains poorly characterized cause of acute coronary syndrome during pregnancy and in postpartum period. The aim of the study was to assess clinical presentation, comorbidities and the outcomes of P-SCAD as compared with spontaneous coronary artery dissection not associated with pregnancy (SCAD).

Design and method:
The study group consisted of 83 women (aged 44.8 ± 9.7y, 36% with hypertension) with a history of at least one pregnancy, including 11 P-SCAD and 72 SCAD cases registered in SCAD-POL Registry. All medical information and angiography images were reviewed. Patients underwent echocardiography for left ventricle ejection fraction (LVEF) assessment and head-to pelvis computed tomography angiography for fibromuscular dysplasia (FMD) screening.

Results:
P-SCAD occurred between 2 and 32 weeks after delivery. Women with P-SCAD were younger (33.1 ± 4.9 vs 46.4 ± 9.1y, p<0.001), were characterized by higher number of pregnancies (3.6 ± 1.2 vs 2.5 ± 1.1, p<0.01) and more often experienced at least 1 miscarriage (63.5% vs 27.8%, p<0.05). The course of pregnancies in the P-SCAD group was more frequently complicated with hypertension (45.5% vs 6,9% p<0.005) and pre-eclampsia (27.3 vs 1.4% p<0.01), non-significantly with diabetes (27.3 vs 6.9%, p=0.07). All P-SCAD patients had at least one pregnancy terminated by caesarean section as compared to 35.8% in SCAD group (p<0.0001).
The prevalence of ST-segment elevation at presentation was comparable between P-SCAD and SCAD patients (43.1% vs. 63.6%, p=ns). However P-SCAD patients more often required coronary bypass grafting (18.2% vs 1.6%, p <0.05). At discharge, left ventricle dysfunction (EF<50%) was observed in 50% of P-SCAD and 29% of SCAD subjects (p=ns). The frequency of FMD was non-significantly higher in P-SCAD subjects (45.5% vs 29.5%). Recurrent SCAD occurred in 2 P-SCAD (18.2%) and 2 SCAD (3.4%) patients.

Conclusions:
P-SCAD patients required more frequently surgical treatment than women with SCAD. Pregnancies in patients with P-SCAD were more often complicated by hypertension, eclampsia and miscarriage, that potentially could have impact on P-SCAD occurrence. Further studies are warranted to explore the results of this hypothesis-generating analysis.
SEVERITY OF EMOTIONAL DISTRESS AND POST-TRAUMATIC STRESS DISORDER AMONG HYPERTENSIVE AND NORMOTENSIVE UKRAINIAN REFUGEE WOMEN IN DIFFERENT LIVING CONDITIONS
Authors: Shalimova Anna , Stoenoiu Maria S. , Cubala Wieslaw J. , Burnier Michel , Persu Alexandre , Narkiewicz Krzysztof 
Published: May 2024

Objective:
The ongoing conflict in Ukraine has led to the exposure of a substantial number of residents to traumatic events and significant stress. A considerable portion of the Ukrainian population, particularly women and children, has sought refuge outside the country to evade the war's impact. Among the Ukrainian refugees in Poland, varying living conditions prevail, potentially influencing the intensity of their stress levels. This study aims to investigate the potential association between living conditions (within or outside dormitories) and the severity of emotional distress and post-traumatic stress disorders (PTSD) in Ukrainian women seeking asylum in Poland.

Design and method:
The study included 98 refugee women living in dormitories and 52 refugees renting apartments in Sopot, Poland. Relevant demographic and clinical data were obtained. Blood pressure (BP) was measured using validated oscillometric devices. Furthermore, subjects filled validated questionnaires for depression, stress and anxiety (DASS) and PTSD (PCL-5). Subjects with and without hypertension were compared for both general and psychological characteristics.

Results:
There were no significant differences in BP levels and the percentage of normotensive and hypertensive patients between two groups. Nevertheless, compared to subjects living in dormitories, refugees leasing apartments exhibited significantly higher DASS scores (30.6±15.7 vs. 19.9±13.4, p<0.0001). This group also included a higher proportion of women with moderate to severe scores for depression (65 vs 32%, p<0.0001), anxiety (83 vs 53%, p<0.001), and stress (69 vs 40%, p<0.0001). Furthermore, refugees renting apartments scored higher for PTSD score (33.5±14.7 vs 21.6±14.1, p<0.0001) particularly regarding intrusion (cluster B, p=0.002), negative alteration in cognition (cluster D, p=0.01) and hyper-arousal (cluster E, p=0.0001) dimensions, and a higher proportion met the criteria of PTSD (60 vs 27%, p<0.0001).

Conclusions:
Refugees residing in leased apartments exhibited comparable rates of hypertension but demonstrated heightened emotional distress and PTSD compared to their counterparts in dormitories. These differences may reflect the positive impact of social support and cohabitation with individuals who share similar traumatic experiences. Further investigations will include in-depth cardiovascular assessment, 24-hour ambulatory BP measurement, reassessment of stress/PTSD questionnaires, and psychiatric consultation.
ESTROGEN DEFICIENCY AGGRAVATES LEFT VENTRICULAR DIASTOLIC DYSFUNCTION AND MITOCHONDRIAL DYSFUNCTION IN IN HEART FAILURE WITH PRESERVED EJECTION FRACTION MICE
Authors: Qi Miaomiao , Wang Qiongying , Yu Jing 
Published: May 2024

Objective:
Postmenopausal estrogen deficiency is associated with an increased cardiovascular risk, hypertension, and heart failure. Mitochondrial dysfunction plays a key role in pathology of heart failure with preserved ejection fraction (HFpEF). We aimed to investigate the effect and mechanism of estrogen deficiency on left ventricular diastolic dysfunction and mitochondrial dysfunction in HFpEF mice.

Design and method:
Eighteen 6-week-old female C57BL/6J mice were randomly divided into normal control group, sham HFpEF group and ovariectomized (OVX) HFpEF group. Mice were fed high-fat diet (HFD) and L-NAME (0.5g/L in drinking water) for 10 weeks to establish a “two-hit” mouse model of HFpEF, showing risk factors such as hypertension, obesity, metabolic dysfunction, and so on. Body weight and blood pressure were measured weekly. Left ventricular (LV) structure and function was monitored by echocardiography. At the end of experiment, animals were starved and histological measures of chamber morphometry and collagen density evaluated. Oxidative stress, mitochondrial function and mitophagy activity were evaluated by western blotting. Statistical analysis was conducted by software SPSS 22.

Results:
Compared with normal group, all HFpEF animals increased body weight and blood pressure (P<0.05). Echocardiographic evaluation revealed persistent preservation of the left ventricular ejection fraction (LVEF) in all HFpEF mice. HFpEF mice exhibited a robust increase in lung weight and indicative of pulmonary congestion. Additionally, cardiac hypertrophy, cardiomyocyte hypertrophy as well as cardiac fibrosis were observed in mice with HFpEF. Compared with sham group, body weight, lung wet weight and cardiac mass significantly increased in OVX group. Mice in OVX group had lower E/A, prolonged IVRT than sham group. Cardiomyocyte hypertrophy and myocardial fiber arrangement were increased, but there were no significant differences in systolic blood pressure, diastolic blood pressure, EF and FS% between the two groups. The expression of ACE-SOD2, NOX4 and Drp1 was significantly higher than that in the sham group, while the expression of Mfn2 protein decreased. Mitophagy-related proteins including LC3, P62, PINK1 and Parkin proteins significantly increased in the OVX group.

Conclusions:
Our findings indicated that estrogen deficiency may aggravate left ventricular diastolic dysfunction and mitochondrial dysfunction in HFpEF mice by activating mitophagy.
LEGACY OF ARTERIAL HYPERTENSION OF BLACK WOMEN WITH A PAST HISTORY OF PREECLAMPSIA
Authors: Ouankou Christian Ngongang , Mfeukeu-kuate Liliane , Njonnou Sylvain Raoul Simeni , Choukem Simeon Pierre 
Published: May 2024

Objective:
Preeclampsia increases the longterm risk of cardiovascular disease. Some of the women affected by this condition remain hypertensive after delivery. This study aimed to determine the prevalence of hypertension using ambulatory BP monitoring (ABPM) and office measurements.

Design and method:
We conducted a cross- sectional study which included women who delivered between January 2015 and June 2021 and were diagnosed and followed up for preeclampsia in three major obstetrical and gynaecologic units in Yaounde. We excluded from the study all women who had a history of chronic hypertension or a known Cardiopathy. Sociodemographic data and past history were recorded, a physical exam,ecg, an ultrasound and ABPM was performed to all participants.

Results:
101 participants were included. The mean age was 30.56 years. 40.6% of women had hypertension (sustained hypertension, with sometime masked hypertension) with ABPM. Sustained hypertension was most common (26.7%), followed by masked hypertension (11.9%) and white-coat hypertension (2%). With office BP measurement, only 28.7% of women would have been diagnosed hypertensive. 29.8% of women had a disadvantageous dipping pattern. Among those that develop chronic hypertension, 18.8% had LVH at electrocardiography, 7.9% had both LVH and an abnormal left ventricular geometry on ultrasound while in normotensive women 1.9% had LVH at Ecg with 1% having both LVH and abnormal geometry at ultrasound. 20.8% of these women were obese during the follow up compared to 11.9% in the normotensive group. We had 26 patients (25,74%) with severe office hypertension and 32,3% were obeses. The fundus was normal in the majority of cases and we had no signs of neurological deficits or impaired cognition. Renal function was always normal or subnormal. Finally, we had two cases of heart failure.

Conclusions:
Many women affected by preeclampsia remain hypertensive after delivery. Masked hypertension and white-coat hypertension are risk factors for future cardiovascular disease and can only be diagnosed with ABPM. It is important to provide adequate follow-up for these women in order to intervene on the factors leading to this outcome.
ROLE OF THR174MET POLYMORPHISM IN THE AGT GENE IN THE DEVELOPMENT OF PREECLAMPSIA
Authors: Kurbanov Bakhodir , Navruzova Rano , Kurbanov Djakhangir , Djamilova Khilola 
Published: May 2024

Objective:
The causes of preeclampsia are multifactorial, complex and not fully understood. Many scientists believe that the main reason for the development of preeclampsia is profound pathological changes in the vascular system and internal organs of a pregnant woman. The purpose of the study was to investigate the role of the Thr174Met polymorphism in the AGT gene in the development of preeclampsia.

Design and method:
The main group included 65 pregnant women with severe preeclampsia, the control group included 60 apparently healthy pregnant women. The age of the women examined ranged from 19 to 41 years.
The material for the study was DNA samples from patients. Isolation of DNA from blood and PCR analysis were carried out using sets of reagents and test systems from Amply Prime Ribo-prep.

Results:
A study of the genotypes of the Thr174Met polymorphism in the AGT gene demonstrated that the Thr/Thr genotype, as well as the Thr allele, was insignificantly less likely to be identified among patients with severe preeclampsia (x2=1.92; p=0.17; RR=1.19; 95%CI:0.73-1.94; OR=1.69; 95%CI:0.80-3.56).
The Thr/Met genotype, on the contrary, statistically significantly prevailed among this category of patients with severe preeclampsia (x2=1.48; p=0.23; RR=0.72; 95%CI:0.72; OR=0.62; 95%CI:0.29-1.34)
The Met/Met genotype also statistically prevailed in severe preeclampsia, relative to patients in the control group (x2=0.29; p=0.61; RR=0.62; 95%CI:0.15-2.56; OR=0.61; 95%CI:0.1-3.71). The heterozygous
An increase in the number of unfavorable Thr/Met and Met/Met genotypes of this locus is associated exclusively with an increase in the risk of developing severe preeclampsia.
The results of the study indirectly indicate the disregulatory effect of unfavorable genotypic variants of this polymorphism on the expression of angiotensinogen which is an important confirmation of the importance of genetic factors to the formation of arterial hypertension and the development of preeclampsia.

Conclusions:
According to the odds ratio, functionally unfavorable genotypes can increase this form of pathology up to 5 times. These data allow us to conclude that the Thr174Met polymorphism of the AGT gene makes a certain contribution to the formation of the genetic structure of predisposition to the development and severity of preeclampsia.
CONTRACEPTION IN WOMEN WITH CARDIOVASCULAR RISK; UNIVERSITY HOSPITAL CENTER OF TLEMCEN, ALGERIA
Authors: Abdrebbi Samira Benbekhti 
Published: May 2024

Objective:
Study the vascular risk in women using oral hormonal contraception.

Design and method:
Descriptive study, interested in studying the blood pressures of women using contraception compared to women not using it. The data were entered and analyzed by Epi-info 6 software.

Results:
In total, 122 women were included in our study, 36 of whom were aged between 21 and 55 years, or 29.5% of cases. Women aged over 60 represented 73.7% of cases; 54.1% were married. There were 98 women users of oral contraception (OC), all ages combined, or 80.3%, compared to 25 women who did not use OC, or 20.5%. The prevalence of hypertension was 74.2% among female OC users; and 68% among women who do not use CO. Epidemiological profile and associated risk factors of hypertensive women using oral contraception: In number of 72. Women aged under 50 represented 23.6% and those aged over 60: 76.4%. A low socioeconomic level was noted for 72.2% and medium for 23.6%. Low educational level: 45.8%, Women who did not have a profession represented 70%. Married women represented 60% of cases and widows 38.9% with an average number of children of five. The average duration of hypertension was 12.15 ± 7.75 years, with a length of service ranging from one year to 40 years. The average duration of contraception was 24 ±7.6 years. Associated risk factors: diabetes 44.4%, hypercholesterolemia: 18.1%, migraine 14%, obesity: 12.5% and tobacco 4.2%.

Conclusions:
Many epidemiological data has investigated the link between oral contraceptive use and elevated blood pressure. In our study, hypertensive women who used oral contraception had significant risk factors; long-standing high blood pressure with an average of more than ten years and taking oral contraception for a long time with an average of more than twenty years. A woman with high blood pressure will be more limited than other women in her choice of contraceptive method.
Comprehensive Noninvasive Fetal Screening by Deep Trio-Exome Sequencing
Authors: Miceikaitė Ieva , Hao Qin , Brasch-Andersen Charlotte , Fagerberg Christina R. , Torring Pernille M. , Kristiansen Britta S. , Ousager Lilian B. , Sperling Lene , Ibsen Mette H. , Löser Katrin , Larsen Martin J. 
Published: May 2024

(Abstracted from N Engl J Med 2023;388:1559–1571
Genetic testing has become an integral part of prenatal care, with many recent advances in clinicians’ ability to detect genetic disorders. Advancements in trio-exome sequencing in particular have shown diagnostic utility.
ARTIFICIAL INTELLIGENCE-ENHANCED ANALYSIS OF RETINAL VASCULATURE IN AGE-RELATED MACULAR DEGENERATION
Authors: Huang Ryan S. , Mihalache Andrew , Popovic Marko M. , Munn Colyn , Melo Isabela Martins , Pecaku Aurora , Friedman Alon , Wong David T. , Muni Rajeev H. 
Published: May 2024

Purpose:
To investigate associations between quantitative vascular measurements derived from intravenous fluorescein angiography (IVFA) and baseline characteristics on optical coherence tomography (OCT) in neovascular age-related macular degeneration (nAMD) patients.

Methods:
The authors prospectively recruited patients with active choroidal neovascularization secondary to AMD over 50 years old, presenting to a single center in Toronto, Canada from 2017 to 2023. Ultra-widefield IVFA images were processed using the artificial intelligence RETICAD FAassist system to extract quantitative information on blood flow, perfusion, and blood–retinal–barrier (BRB) permeability. Associations between IVFA parameters with functional and anatomical outcomes were examined using univariable and multivariable regression models.

Results:
Eighty-one nAMD eyes and seven healthy control eyes were included. Compared with healthy controls, BRB permeability in the central and peripheral retina was significantly higher in nAMD patients (P < 0.001). On univariable analysis, BRB permeability measured centrally was significantly associated with central macular thickness (P = 0.035), whereas perfusion and blood flow measured centrally were significantly associated with macular volume (P = 0.043 and 0.037, respectively). On multivariable analysis, BRB permeability remained significantly associated with central macular thickness (P = 0.026).

Conclusion:
Central BRB permeability measured on IVFA was significantly associated with baseline central macular thickness in nAMD patients. Future work should longitudinally explore associations between IVFA parameters and clinical characteristics in diverse nAMD populations.
Implications of intraocular ossification after repeated vitrectomies: exploring a novel surgical intervention.
Authors: Hershko Sarah , Van den Heurck Jonas , Neetens Ingrid , Claes Carl 
Published: May 2024

Purpose:
To describe the management of a case of retinal detachment combined with extensive intraocular ossification (IOO) using an innovative surgical technique of repair.

Methods:
Surgical procedures involved open sky approach and the use of a bone punch instrument for the removal of the ossified structures.

Results:
The applied surgical techniques effectively removed the ossified material, contributing to retinal reattachment and preservation of functional vision. Particularly challenging was the rapid rebleeding and re-ossification observed after the removal of intraocular ossification, a phenomenon not yet described in the existing literature. This necessitated an additional vitrectomy using a similar approach.

Conclusion:
This paper highlights the challenges associated with IOO and the innovative surgical approach used for its management. The utilization of a bone punch instrument, although effective, necessitates caution due to its non-standard application in intraocular surgery. Further investigation and clinical experience are essential to refine the methodology and evaluate long-term outcomes in IOO management.
Transcutaneous electrical acupoint stimulation reduces postoperative patients’ length of stay and hospitalization costs: a systematic review and meta-analysis
Authors: Liu Yilong , Fan Jiefu , Zhang Xiaoqing , Xu Wenping , Shi Zhiwen , Cai Jiarong , Wang Peiqin 
Published: May 2024

Objective:
To study the effects of transcutaneous electrical acupoint stimulation (TEAS) on length of stay (LOS) and hospitalization costs in postoperative inpatients.

Methods:
Two researchers collectively searched PubMed, Embase, Cochrane Library, China Network Knowledge Infrastructure, and Wanfang Database. The search time was set from the beginning to 25 April 2023, to identify randomized controlled trials articles that met the criteria. Statistical analyses were performed using the Stata software (version 16.0). The risk of bias was assessed using the Cochrane risk-of-bias tool, and publication bias was evaluated using a funnel plot and Egger’s test. The quality of evidence was assessed according to the Grading of Recommendations Assessment, Development, and Evaluation approach.

Results:
Thirty-four randomized controlled trials were included. The main results showed that TEAS reduced hospitalization costs [standardized mean difference (SMD)=−1.92; 95% CI: −3.40, −0.43), LOS (SMD=−1.00; 95% CI: −1.30, −0.70) and postoperative LOS (SMD=−0.70; 95% CI: −0.91, −0.49] in postoperative patients. Subgroup analyses further revealed that TEAS was effective in reducing both the overall and postoperative LOS in patients undergoing multiple surgical procedures. It is worth noting that the observed heterogeneity in the results may be attributed to variations in surgical procedures, stimulation frequencies, and stimulation points utilized in different trials.

Conclusions:
TEAS can help postoperative patients reduce their LOS and hospitalization cost. However, considering the bias identified and heterogeneity, the results of this review should be interpreted with caution.