bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design and characterization of a polymeric MRI contrast agent based on PVA for in vivo living‐cell tracking
Authors: Tachibana Yoichi , Enmi Jun‐ichiro , Mahara Atsushi , Iida Hidehiro , Yamaoka Tetsuji 
Published: May 2010

AbstractA novel water‐soluble MRI contrast agent for in vivo living cell tracking was developed. Unlike the conventional in vivo cell tracking system based on superparamagnetic iron oxide beads, the newly developed contrast agent is eliminated from the body when the contrast agent exits the cells upon cell death, which makes living cell tracking possible. The contrast agent is composed of gadolinium chelates (Gd–DOTA) and a water‐soluble carrier, poly(vinyl alcohol) (PVA), which is known to interact with cells and tissues very weakly. Since the Gd–PVA was not taken up by cells spontaneously, the electroporation method was used for cell labeling. The delivered Gd–PVA was localized only in the cytosolic compartment of growing cells with low cytotoxicity and did not leak out of the living cells for long periods of time. This stability may be due to the weak cell‐membrane affinity of Gd–PVA, and did not affect cell proliferation at all. After cell labeling, signal enhancement of cells was observed in vitro and in vivo. These results indicate that Gd–PVA can visualize only the living cells in vivo for a long period of time, even in areas deep within large animal bodies. Copyright © 2010 John Wiley & Sons, Ltd.
The efficacy and safety of lenalidomide plus dexamethasone in relapsed and/or refractory multiple myeloma patients with impaired renal function
Authors: Dimopoulos Meletios , Alegre Adrian , Stadtmauer Edward A. , Goldschmidt Hartmut , Zonder Jeffrey A. , de Castro Carlos M. , Masliak Zvenyslava , Reece Donna , Olesnyckyj Marta , Yu Zhinuan , Weber Donna M. 
Published: May 2010

AbstractBACKGROUND:In patients with multiple myeloma, renal impairment (RI) at the time of diagnosis is associated with poor survival. To the authors' knowledge, the current retrospective analysis presented is the first to assess the impact of various degrees of renal dysfunction on safety and efficacy outcomes in a large cohort of patients with relapsed and/or refractory multiple myeloma who received treatment with lenalidomide plus dexamethasone.METHODS:Three hundred fifty‐three patients from 2 large phase 3 trials were randomized to receive lenalidomide (25 mg) plus dexamethasone (40 mg). For the purpose of this analysis, RI was defined according to the calculated creatinine clearance (CLCr) level as follows: mild or no RI (CLCr ≥ 60 mL/minute), moderate RI (CLCr from ≥ 30 mL/minute to <60 mL/minute), and severe RI (CLCr <30 mL/minute).RESULTS:The RI subgroups did not differ significantly in terms of the overall response rate (range, 50%‐64%) or response quality (very good partial response or better, 27%‐37%). In all RI subgroups, the time to progression and progression‐free survival did not differ significantly compared with the mild or no RI group. Patients with RI experienced an increased incidence of thrombocytopenia, required more frequent lenalidomide dose reduction or interruption, and had shorter overall survival than patients with mild or no RI (P = .006). Lenalidomide plus dexamethasone led to improvement in renal function in the majority of patients.CONCLUSIONS:The results from this study indicated that, with careful monitoring of the CLCr level and adverse events as well as appropriate dose adjustments, lenalidomide plus dexamethasone is an effective and well tolerated treatment option for patients with multiple myeloma who have RI. Cancer 2010. © 2010 American Cancer Society.
Publication and consumption of caBIG data services using .NET
Authors: Humphrey Marty , Li Jie , Beekwilder Norm 
Published: May 2010

AbstractThe cancer Biomedical Informatics Grid (caBIG) is revolutionizing the way medical researchers share information and collaborate. A key to caBIG's continued success will be interoperability. However, to date, only a single code base (in Java) has been used to create a set of tools and run‐time services for caBIG. This paper presents the first significant exploration into the use of Microsoft's .NET Framework and Visual Studio for caBIG. Given its substantial existing community, a .NET‐based set of tools for caBIG can significantly increase the pool of qualified software designers and developers for caBIG. Arguably more importantly, a second development foundation could facilitate revisiting a broad set of design decisions made to date in caBIG that have perhaps been unduly based directly or indirectly on a single underlying software technology. We begin by describing issues we have encountered in building relatively simple .NET‐based clients to existing caBIG services. Next, we describe how we leverage Microsoft ADO.NET Data Services as the foundation for caBIG Data Services, in particular for the caBIO data set. We find that ADO.NET Data Services has a uniquely strong potential to facilitate rapid development and deployment. We conclude with a discussion of the roadmap of our project's future activities. Copyright © 2010 John Wiley & Sons, Ltd.
Group cognitive behaviour therapy for adults with Asperger syndrome and anxiety or mood disorder: a case series
Authors: Weiss Jonathan A. , Lunsky Yona 
Published: May 2010

AbstractIndividuals with Asperger syndrome are at increased risk for mental health problems compared with the general population, especially with regard to mood and anxiety disorders. Generic mental health services are often ill‐equipped to offer psychotherapeutic treatments to this population, and specialized supports are difficult to find. This case series used a manualized cognitive behaviour therapy group programme (Mind Over Mood) with three adults diagnosed with Asperger syndrome, who were each unable to access psychotherapy through mainstream mental health services. This review highlights the benefits of a cognitive behaviour therapy (CBT) group approach for adults with Asperger syndrome and suggests some potential modifications to traditional CBT provision. Copyright © 2010 John Wiley & Sons, Ltd.Key Practitioner Message:• As a group, adults with Asperger syndrome are at high risk for anxiety disorders and depression.• Cognitive behaviour therapy can be adapted to help adults with Asperger syndrome cope with anxiety or depression.• Group cognitive behaviour therapy for adults with Asperger syndrome may hold a number of advantages to individual therapy.
Adult attachment insecurity and narrative processes in psychotherapy: an exploratory study
Authors: Daniel Sarah I. F. 
Published: May 2010

AbstractDifferent types of client attachment insecurity may affect the psychotherapeutic process in distinct ways. This exploratory study compared the in‐session discourse of clients with dismissing and preoccupied attachment states of mind on Adult Attachment Interviews conducted prior to therapy in the context of a randomized clinical trial of psychoanalytic and cognitive–behavioural psychotherapy for bulimia nervosa. In a subsample of six sessions from each of eight therapy dyads, preoccupied clients were found to talk more and have longer speaking turns than dismissing clients, who in turn generated more pauses. Using the Narrative Processes Coding System, preoccupied clients were found to show more narrative initiative; whereas, differences in terms of narrative process modes were not as clearly interpretable. Contrary to expectations, the two insecure states of mind were equally different in the relationship‐focused psychoanalytic therapy and in the symptom‐focused cognitive–behavioural therapy. Suggestions for further investigations of the in‐session discourse of clients with different attachment states of mind are given. Copyright © 2010 John Wiley & Sons, Ltd.Key Practitioner Message:• Client adult attachment patterns are likely to affect psychotherapeutic processes.• Client insecure dismissing and insecure preoccupied attachment may be detectable in distinct in‐session discourse styles.• More research is needed on in‐session manifestations of client attachment patterns and their relation to therapeutic outcome.
Amplifications and applications of Pennebaker's analogic to digital model in health promotion, prevention, and psychotherapy
Authors: De Giacomo Piero , L'Abate Luciano , Pennebaker James W. , Rumbaugh Duane 
Published: May 2010

AbstractThis article expands on James Pennebaker's original model in expressive writing, going from an Analogically unclear and unspecified experiential mass (A) to a Digital specification in words (D). Amplifications of this model are found in philosophy, psychodynamic theory and in Salience learning theory. Applications of this model involve expressive writing, going from ambiguous experiences (A) to using the Dictionary (D), and responding to Sentences with strong psychological impact (S). The undefined and ill‐defined analogical mass is constituted by traumas in Pennebaker's work and hurt feelings in L'Abate's and Vangelisti's works. Implications of these approaches for the practice of health promotion, prevention and psychotherapy are discussed. Copyright © 2010 John Wiley & Sons, Ltd.Key Practitioner Message:• This article points to the presence of internal feelings that are produced by abusive, painful, hurtful or of negatively perceived events in a person's life at various levels of awareness, from deeply unconscious, semi‐conscious, conscious but not spoken, and conscious and spoken.• Consequently, there are at least four different techniques that can be used by practitioners to elicit and facilitate the emergence of these feelings through. • Traditional talk‐based face‐to‐face psychotherapy focused mostly on those feelings rather than other distracting or tangential topics. • Expressive writing, the Pennebaker's paradigm about past traumas for 15 to 20 minutes a day for 4 consecutive days. • Programmed distance writing with interactive practice exercises or work‐books specifically focused on hurt feelings using a matching Informed Consent Form to be signed by participants before introducing any therapeutic approach to elicit hurt feelings. • Administration of sentences with strong psychological impact that may be related to hurt feelings.
Development of a novel, cell‐based chemical screen to identify inhibitors of intraphagosomal lipolysis in macrophages
Authors: VanderVen Brian C. , Hermetter Albin , Huang Amy , Maxfield Fredrick R. , Russell David G. , Yates Robin M. 
Published: May 2010

AbstractMacrophages play a central role in tissue homeostasis and the immune system. Their primary function is to internalize cellular debris and microorganisms for degradation within their phagosomes. In this context, their capacity to process and sequester lipids such as triacylglycerides and cholesteryl esters makes them key players in circulatory diseases, such as atheroclerosis. To discover new inhibitors of lipolytic processing within the phagosomal system of the macrophage, we have developed a novel, cell‐based assay suitable for high‐throughput screening. We employed particles carrying a fluorogenic triglyceride substrate and a calibration fluor to screen for inhibitors of phagosomal lipolysis. A panel of secondary assays were employed to discriminate between lipase inhibitors and compounds that perturbed general phagosomal trafficking events. This process enabled us to identify a new structural class of pyrazole‐methanone compounds that directly inhibit lysosomal and lipoprotein lipase activity. © 2010 International Society for Advancement of Cytometry
Tubulolobular carcinoma of the breast with grooved and cerebriform nuclei: Failure to identify this specific subtype in a case during routine fine needle aspiration cytology and histopathological diagnosis
Authors: Das Dilip K. , Haji Bahia I. , Abdeen Suad M. , John Bency , Sheikh Mehraj , Al‐Bader Ibtisam , Behbehani Abdulla I. F. 
Published: May 2010

AbstractTubulolobular carcinoma (TLC) is a rare tumor of the breast in which histologic features of both tubular and lobular carcinoma are combined. We report a case of TLC, in which the specific subtype was missed at routine cytologic and histopathological examination. A 69‐year‐old woman presented with a right breast lump. Imaging studies indicated a malignant lesion in right upper quadrant. Routine fine needle aspiration (FNA) cytology diagnosis was a duct cell carcinoma (small cell type). In a setting of cystic thyroid lesions, presence of excessive nuclear grooves, and rare intranuclear cytoplasmic inclusion, metastatic papillary thyroid carcinoma was also considered. However, both these possibilities were not supported by immunocytochemical findings (estrogen receptor+, thyroglobulin−, and chromogranin−). The histopathology diagnosis was invasive duct cell carcinoma. Review of FNA smears and paraffin sections led to the diagnosis of TLC, which was supported by positive immunohistochemical stainings for markers like e‐cadherin and β‐catein. Diagn. Cytopathol. 2011;39:54–59. © 2010 Wiley‐Liss, Inc.
Usefulness of Claudin 4 in the cytological diagnosis of serosal effusions
Authors: Lonardi Silvia , Manera Calogero , Marucci Raffaella , Santoro Amerigo , Lorenzi Luisa , Facchetti Fabio 
Published: May 2010

AbstractThe identification of metastatic cells in serous effusions has prognostic and therapeutic implications, thus leading to a continuous search for improvement of the existing diagnostic procedures, including immunocytochemistry. To evaluate the usefulness of an antibody recognizing the tight junction‐associated protein Claudin 4 in detecting metastatic tumor cells and in the differential with reactive and neoplastic mesothelium, we stained 345 cases of benign and neoplastic serous effusions obtained from pleura, peritoneum, and pericardium. Two‐hundred and twenty‐eight of 230 cases (99.1%) of epithelial metastasis of different origin were strongly stained by anti‐Claudin 4, whereas all cases of reactive mesothelitis (78) and malignant mesothelioma (37) were negative. With the exception of a single case of ovarian carcinoma hypercalcemic‐type, all tumors originating from the anatomical sites that most frequently metastasize to the serosae, including lung (61), breast (23), female genital tract (67), gastrointestinal tract (27), and peritoneum (6), were found to be positive. Claudin 4 was also extremely useful in detecting single‐tumor cells dispersed among heavy inflammatory reaction. Because of its high sensitivity (99.1%) and specificity (100%), Claudin 4 might be used as an ideal “single‐shot” marker for the identification of metastatic epithelial cells in serous effusions. Diagn. Cytopathol. 2011;39:313–317. © 2010 Wiley‐Liss, Inc.
Conjunctival metastasis from Kaposi's sarcoma: A case report
Authors: Cano Roberto Gabriel Albìn , Cardenas Juan Carlos Perez 
Published: May 2010

AbstractWe present one case of Kaposi's sarcoma with conjunctive metastasis. The case describes a 71‐years‐old white male, who was admitted at hospital by malaise, slight pain on the left eyelid, nodular lesion of the left leg, and enlarge lymph nodes in inguinal regions. The conjunctive neoplasms are rare, and we most frequently can see the pigment ones fallowed by squamous epithelials and lymphomas. The primary Kaposi's sarcoma (KS) from conjunctiva is a rare tumor and it has increased by AIDS emergence and immunosuppresion. The appearance of conjunctival metastasis from KS is a stand out event. Diagn. Cytopathol. 2011;39:128–131. © 2010 Wiley‐Liss, Inc.
Expression of Ki‐67 and p27Kip1 in fine‐needle aspirates from breast carcinoma and benign breast diseases
Authors: Ramljak Vesna , Sučić Mirna , Vrdoljak Danko Velemir , Borojević Nikola 
Published: May 2010

AbstractCell atypia in breast fine needle aspiration (FNA) can introduce some diagnostic difficulties. Molecules reflecting proliferative cell potential, such as Ki‐67 and p27Kip1, can help in recognizing the true biological nature of a cell. Thus, the objective of the study was to analyze the difference in Ki‐67 and p27Kip1 cell immunoexpression in breast FNA specimens between fibroadenomas, fibrocystic changes (FCC) with atypia, and breast carcinoma. Microscopic analyses of cell cytomorphology and Ki‐67 and p27Kip1 breast cell immunoexpression were done after standard Pappenheim and immunocytochemical staining (labeled streptavidin‐biotin, LSAB) method in autostainer DakoCytomation TechMate™. The study included 50 patients with breast carcinoma, 20 patients with fibroadenoma, and 20 patients with FCC with atypia. High Ki‐67 and low or absent p27Kip1 were found in most patients with breast carcinoma, while majority of FCC with atypia were characterized by low Ki‐67 and moderate to high p27Kip1 cell immunoexpression. Majority of fibroadenomas were associated with low Ki‐67 and low to moderate p27Kip1 cell immunoexpression indicating progressive decrease in cell cycle inhibition, but still not so high proliferative activity as in carcinoma. However, although statistically significant difference for Ki‐67 and p27Kip1 was found between breast lesions in our study, the large ranges observed for each marker make them essentially useless for better cytological diagnosis in a single case. Regarding their opposite role in cell cycle, inverse correlation of Ki‐67 and p27Kip1 was noticed. Poorly differentiated carcinoma cells had mostly high Ki‐67 andlow p27Kip1 cell immunoexpression. Diagn. Cytopathol. 2011;39:333–340. © 2010 Wiley‐Liss, Inc.
Diagnostic usefulness of EMA, IMP3, and GLUT‐1 for the immunocytochemical distinction of malignant cells from reactive mesothelial cells in effusion cytology using cytospin preparations
Authors: Ikeda Katsuhide , Tate Genshu , Suzuki Takao , Kitamura Takashi , Mitsuya Toshiyuki 
Published: May 2010

AbstractTo differentiate reactive mesothelial cells (RMs) from metastatic carcinoma and malignant mesothelioma (MM) in effusion cytology is crucial for the cytologic diagnosis and the management of the patients. In the present study, the immunocytochemical staining profile of the epithelial membrane antigen (EMA), the insulin‐like growth factor‐II mRNA‐binding protein 3 (IMP3), and the glucose transporter‐1 (GLUT‐1) was examined to distinguish RMs from malignant cells. A total of 171 pleural (n = 87) and peritoneal (n = 84) effusion specimens, including 50 benign effusions with RMs, 11 MM effusions, and 110 metastatic malignant effusions, were evaluated for immunocytochemistry. EMA, IMP3, monoclonal GLUT‐1, and polyclonal GLUT‐1 immunoreactivity were observed in 26.0%, 6.0%, 20.0%, and 18.0% of RMs, respectively. In contrast to RMs, the immunoreactivity in MM was 100%, 36.4%, 100%, and 90.9%; adenocarcinoma (AC) was 100%, 80.8%, 81.7%, and 72.1%; squamous‐cell carcinoma was 83.3%, 83.3%, 83.3%, and 66.7%. EMA, IMP3, mGLUT‐1, and pGLUT‐1 expressions were observed in 98.4%, 65.6%, 88.5%, and 75.4% in the pleural effusion with malignant cells, and 100%, 88.3%, 78.3%, and 71.7% in ascites containing malignant cells, respectively. The findings of the present study indicate that the immunocytochemical staining for EMA, IMP3, and GLUT‐1 is a useful diagnostic tool for distinguishing effusions containing malignant cells from those that contain benign cells, and in particular, we suggest that the combination of mGLUT‐1 and EMA, and IMP3 and EMA are extremely useful in pleural effusion and in ascites, respectively. Diagn. Cytopathol. 2011;39:395–401. © 2010 Wiley‐Liss, Inc.
Combining cytomorphology and serology for the diagnosis of cat scratch disease
Authors: Youssef Dima , Shams Wael E. , El Abbassi Adel , Moorman Jonathan P. , Al‐Abbadi Mousa A. 
Published: May 2010

AbstractCat scratch disease (CSD) is a self limited zoonotic disease that presents most commonly as a regional lymphadenopathy. We are reporting a case of a 25‐year‐old male patient who presented with fever and large right inguinal lymphadenopathy. The diagnosis of cat scratch disease was confirmed based on the characteristic cytopathological features on aspirate smears from the lymph node and the serological titers for Bartonella henselae. This case report emphasizes the importance of combining Bartonella serology, and cytopathology in the diagnostic work‐up of febrile lymphadenopathy and suspected CSD since the culture of this organism is arduous. Diagn. Cytopathol.2011;39:210–213. © 2010 Wiley‐Liss, Inc.
Fine‐needle aspiration biopsy of low‐grade cribriform cystadenocarcinoma of the salivary gland
Authors: Nakazawa Tadao , Kondo Tetsuo , Yuminomochi Tsutomu , Nakazawa Kumiko , Ishii Yoshio , Mochizuki Kunio , Kawasaki Tomonori , Yamane Tetsu , Miyata Masanori , Motosugi Utaroh , Katoh, Ryohei 
Published: May 2010

AbstractLow‐grade cribriform cystadenocarcinoma (LGCCC) is a rare tumor, defined in the 2005 WHO classification as a primary salivary duct tumor. Previously, the neoplasm had been recognized as a variant of salivary duct carcinomas. A 56‐year‐old Japanese woman noticed a mass in the left subaural region. On radiological examinations, a multicystic tumor was seen in the left parotid gland. Fine‐needle aspiration biopsy was performed. The smears revealed several characteristic cytologic features. The tumor cells were arranged in irregular overlapping and showed inconspicuous nuclear atypia with variable‐sized and irregularly shaped cytoplasmic vacuoles. Based on these findings, a cystic tumor with uncertain malignancy was diagnosed. A parotidectomy was performed, because the tumor was slowly growing and contained solid components on the radiological images. Based on the histologic findings, along with immunohistochemistry, LGCCC was diagnosed based on resemblance to breast low‐grade ductal carcinoma in situ and intraductal proliferation of tumor cells. This is the first report of the cytomorphological findings of LGCCC. Diagn. Cytopathol.2011;39:218–222. © 2010 Wiley‐Liss, Inc.
Improved sensitivity over time with rapid prescreening in gynecologic cytology
Authors: Dudding Nick , Renshaw Andrew A. , Ellis Kay 
Published: May 2010

AbstractRapid prescreening (RPS) is a powerful tool to measure and improve performance in the cytology laboratory. Long‐term use of RPS has been shown to result in improved sensitivity and precision in routine screening. The effect of long‐term RPS on RPS itself is not known. We compared the sensitivity of 100% RPS of Surepath™ liquid‐based cytology over a 4‐year period in a laboratory of 11 cytotechnologists (CTs). In comparison with the first 2 years, RPS for the laboratory showed a significant increase in sensitivity for all abnormalities (72.2% vs. 67.3%, P < 0.001) and ASCUS + LSIL (68.5% vs. 62.1%, P < 0.001). For individual CTs s, the lowest sensitivity for all abnormalities increased from 59.0 to 63.2%, whereas, for HSIL, it increased from 71.4% to 75.0%. We conclude that long‐term use of RPS leads to significant increase in the sensitivity of RPS. Diagn. Cytopathol. 2011;39:428–430. © 2010 Wiley‐Liss, Inc.
Identification of etamiphylline and metabolites in equine plasma and urine by accurate mass and liquid chromatography/tandem mass spectrometry
Authors: Wieder M. E. , Brown P.R. , Grainger L. , Teale P. 
Published: May 2010

AbstractEtamiphylline camsylate (Millophylline V) was administered intravenously to two horses at a dose of 2.8 mg/kg. Urine and blood samples were taken up to 32 h post administration. Unhydrolyzed plasma and urine was extracted using solid phase extraction (SPE). The identity of the parent drug and metabolites was confirmed using a linear ion trap mass spectrometer and accurate mass analysis on an orbitrap mass spectrometer. Desethyletamiphylline (molecular weight 251) was the main metabolite observed in the urine and plasma samples and resulted from the N‐deethylation of etamiphylline. The second metabolite detected in urine and plasma resulted from the demethylation of etamiphylline (molecular weight 265). The third minor metabolite detected in urine was proposed to have resulted from a simultaneous N‐deethylation and demethylation of etamiphylline (molecular weight 238). Copyright © 2010 John Wiley & Sons, Ltd.