bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ligament of Barkow of the craniocervical junction: its anatomy and potential clinical and functional significance
Authors: Tubbs R. Shane , Dixon Joshua , Loukas Marios , Shoja Mohammadali M. , Cohen-Gadol Aaron A. 
Published: June 2010

Object
Knowledge of the anatomy of the ligaments that unite the head with the neck is important to the clinician who treats patients with lesions in this region. Although the anatomy and function of these ligaments have been well described, those of the Barkow ligament (BL) have yet to be studied.

Methods
Via an anterior approach, 13 unembalmed adult cadavers underwent dissection of the craniocervical junction with special attention to the presence, anatomy, and function of the BL.

Results
The BL was found in 92.3% of specimens. The attachment of each ligament onto the medial aspect of the occipital condyle was consistent and just anterior to the attachment of the alar ligaments. In 75% of specimens, there was some connection between the BL and the anterior atlantooccipital membrane. Connections between other adjacent ligamentous structures were not identified. The average width, length, and thickness of the BL were 4, 2.5, and 3.5 mm, respectively. With ranges of motion of the craniocervical junction, only extension of the atlantooccipital joint produced tension in the BL. The mean tension to failure of the ligament was 28 N. Statistical analysis revealed no significant difference in width, length, and thickness of the ligaments based on sex.

Conclusions
The BL was found in all but 1 of our specimens. This ligament appears to resist extension of the atlantooccipital joint and may be synergistic with the anterior atlantooccipital membrane. Interestingly, the function of this ligament as found in this study relies on the integrity of the transverse ligament. Knowledge of this ligament may aid in further understanding craniocervical stability and help in differentiating normal from pathological tissue using imaging modalities.
Nonarticular Tenderness and Functional Status in Patients with Diffuse Idiopathic Skeletal Hyperostosis
Authors: MADER REUVEN , NOVOFASTOVSKI IRINA , ROSNER EHUD , ADAWI MUHAMMAD , HERER PAULA , BUSKILA DAN 
Published: June 2010

Objective.To investigate the degree of nonarticular tenderness and functional status in patients with diffuse idiopathic skeletal hyperostosis (DISH). We assessed these variables’ correlation with their clinical, radiographic, and constitutional measurements and with metabolic syndrome (MS).Methods.Eighty-seven patients with DISH were compared with 65 controls without DISH. Examination of nonarticular tenderness was performed by thumb palpation. Tenderness was scored for the 18 fibromyalgia tender points (TP), and 4 control points. Nonarticular tenderness was expressed by the number of TP and by the total tenderness score (TTS). The Short Health Assessment Questionnaire (HAQ II) was administered to all participants. Clinical and laboratory data were collected from all patients. Patients were classified as having MS by both the National Cholesterol Education Program and World Health Organization definitions.Results.There was a statistically significant difference in TTS between controls and patients with DISH. The mean tenderness of many individual TP was significantly higher in the DISH group compared with the control group. TP counts, TTS, and body mass index (BMI) positively correlated with the HAQ II. There was a linear trend in intensity of T-spine bony bridges (BB) and the total number of TP as well as many individual TP. Patients with DISH were more likely to be affected by MS. No correlation was found between TP count, TTS, and MS.Conclusion.Patients with DISH have a lower pain threshold than patients who do not have DISH. TP count and TTS correlate with the functional status, BMI, waist circumference, and high-grade BB. No correlation was observed between pain threshold and MS.
Evaluation of 21-Numbered Circle and 10-Centimeter Horizontal Line Visual Analog Scales for Physician and Parent Subjective Ratings in Juvenile Idiopathic Arthritis
Authors: FILOCAMO GIOVANNI , DAVÌ SERGIO , PISTORIO ANGELA , BERTAMINO MARTA , RUPERTO NICOLINO , LATTANZI BIANCA , CONSOLARO ALESSANDRO , MAGNI-MANZONI SILVIA , GALASSO ROBERTA , VARNIER GIULIA CAMILLA , MARTINI ALBERTO , RAVELLI ANGELO 
Published: June 2010

Objective.To evaluate the measurement properties of 21-numbered circle visual analog scales (VAS) and traditional 10-cm horizontal line VAS for physician and parent subjective ratings in children with juvenile idiopathic arthritis (JIA).Methods.We studied 2 patient samples in whom physician global rating of overall disease activity, parent global rating of the child’s overall well-being, and parent rating of intensity of child’s pain were performed using traditional 10-cm horizontal line VAS (n = 397) or 21-numbered circle VAS (n = 471). The measurement performances of the 2 VAS formats were examined by assessing construct validity, score distribution, responsiveness to change over time, and minimal clinically important difference (MCID).Results.Most Spearman correlations with other JIA outcome measures yielded by 21-numbered circle VAS were greater than those obtained with 10-cm horizontal line VAS, revealing that the circle VAS format has better construct validity. Ceiling effects (i.e., score = 0) for physician and parent global ratings were 43.7% and 32.9%, respectively, on 21-numbered circle VAS, and 31.6% and 35.3%, respectively, on 10-cm horizontal line VAS. Responsiveness of 21-numbered circle VAS was good (standardized response mean > 0.8) or moderate (standardized response mean > 0.6) among patients classified as improved or worsened, respectively, by the physician or the parent. Overall, MCID values for 21-numbered circle VAS tended to be greater for worsening than for improvement.Conclusion.The 21-numbered circle VAS are a suitable alternative to the 10-cm horizontal line VAS and may facilitate incorporation of physician and parent subjective ratings in standard clinical practice.
Woodpecker Densities in the Big Woods of Arkansas
Authors: Krementz David G. , Luscier Jason D. 
Published: June 2010

AbstractSightings of the now-feared-extinct ivory-billed woodpecker Campephilus principalis in 2004 in the Big Woods of Arkansas initiated a series of studies on how to best manage habitat for this endangered species as well as all woodpeckers in the area. Previous work suggested that densities of other woodpeckers, particularly pileated Dryocopus pileatus and red-bellied Melanerpes carolinus woodpeckers, might be useful in characterizing habitat use by the ivory-billed woodpecker. We estimated densities of six woodpecker species in the Big Woods during the breeding seasons of 2006 and 2007 and also during the winter season of 2007. Our estimated densities were as high as or higher than previously published woodpecker density estimates for the Southeastern United States. Density estimates ranged from 9.1 to 161.3 individuals/km2 across six woodpecker species. Our data suggest that the Big Woods of Arkansas is attractive to all woodpeckers using the region, including ivory-billed woodpeckers.
Application of a Double-Observer Aerial Line-Transect Method to Estimate Brown Bear Population Density in Southwestern Alaska
Authors: Walsh Patrick , Reynolds Joel , Collins Gail , Russell Brook , Winfree Michael , Denton Jeffrey 
Published: June 2010

AbstractBrown bear Ursus arctos population density was estimated for a 21,178-km2 study area in southwest Alaska. Estimates were obtained using an aerial line-transect method that allows for peak detection to be both off the transect line and less than 100%. Data collection required five small aircraft with 2-person crews. Surveys were flown in 10-d windows to capture the period after den emergence but prior to full green-up. Surveys were flown in two consecutive years in order to detect sufficient bear groups to support the estimation. The study detected 197 bear groups (330 bears) in 969 aerial transects averaging 24.8 km long and with a strip width of 728 m. Estimated population density in the study area was 40.4 bears/1,000 km2 (95% CI  =  31.4–54.5); estimated density of independent bears was 27.3 bears/1,000 km2 (95% CI  =  21.4–34.4). Assuming similar estimate precision, repeating the survey could detect population changes of approximately 38% or larger with a power of 80%. We find the method described here suitable for regions of relatively high bear population densities or detection rates.
Ecto-5′-Nucleotidase (CD73) Attenuates Allograft Airway Rejection through Adenosine 2A Receptor Stimulation
Authors: Ohtsuka Takashi , Changelian Paul S. , Bouïs Diane , Noon Kathleen , Harada Hiroaki , Lama Vibha N. , Pinsky David J. 
Published: June 2010

AbstractThere are multiple drivers of leukocyte recruitment in lung allografts that contribute to lymphocytic bronchitis (LB) and bronchiolitis obliterans (BO). The innate mechanisms driving (or inhibiting) leukocyte trafficking to allografts remain incompletely understood. This study tested the hypothesis that CD73 (ecto-5′nucleotidase), an enzyme that catalyzes the conversion of AMP to adenosine, is a critical negative regulator of LB and BO. Implantation of tracheal allografts from wild type (WT) mice into CD73−/− recipients revealed a striking increase in airway luminal obliteration at 7 d (62 ± 4% and 47 ± 5% for CD73−/− and WT allograft recipients, respectively; p = 0.046). There was also a concordant increase in CD3+ lymphocytic infiltration (523 ± 41 cells and 313 ± 43 cells for CD73−/− and WT allograft recipients, respectively; p = 0.013). Because real-time PCR revealed a 43-fold upregulation of mRNA for the adenosine A2A receptor (A2AR) in WT allografts compared with WT isografts (p = 0.032), additional experiments were performed to determine whether the protective effect of CD73 was due to generation of adenosine and its stimulation of the A2AR. Treatment of WT recipients with an A2AR agonist significantly reduced CD3+ lymphocyte infiltration and airway luminal obliteration; similar treatment of CD73−/− recipients rescued them from LB and airway obliteration. These data implicate CD73 acting through adenosine generation and its stimulation of the A2AR as a critical negative modulator of lymphocyte recruitment into airway allografts. The CD73/adenosine axis might be a new therapeutic target to prevent BO.
Concomitant Tumor and Autoantigen Vaccination Supports Renal Cell Carcinoma Rejection
Authors: Herbert Nicolás , Haferkamp Axel , Schmitz-Winnenthal Hubertus F. , Zöller Margot 
Published: June 2010

Abstract
Efficient tumor vaccination frequently requires adjuvant. Concomitant induction of an autoimmune response is discussed as a means to strengthen a weak tumor Ag-specific response. We asked whether the efficacy of dendritic cell (DC) vaccination with the renal cell carcinoma Ags MAGE-A9 (MAGE9) and G250 could be strengthened by covaccination with the renal cell carcinoma autoantigen GOLGA4. BALB/c mice were vaccinated with DC loaded with MHC class I-binding peptides of MAGE9 or G250 or tumor lysate, which sufficed for rejection of low-dose RENCA-MAGE9 and RENCA-G250 tumor grafts, but only retarded tumor growth at 200 times the tumor dose at which 100% of animals will develop a tumor. Instead, 75–100% of mice prevaccinated concomitantly with Salmonella typhimurium transformed with GOLGA4 cDNA in a eukaryotic expression vector rejected 200 times the tumor dose at which 100% of animals will develop tumor. In a therapeutic setting, the survival rate increased from 20–40% by covaccination with S. typhimurium-GOLGA4. Autoantigen covaccination significantly strengthened tumor Ag-specific CD4+ and CD8+ T cell expansion, particularly in peptide-loaded DC-vaccinated mice. Covaccination was accompanied by an increase in inflammatory cytokines, boosted IL-12 and IFN-γ expression, and promoted a high tumor Ag-specific CTL response. Concomitant autoantigen vaccination also supported CCR6, CXCR3, and CXCR4 upregulation and T cell recruitment into the tumor. It did not affect regulatory T cells, but slightly increased myeloid-derived suppressor cells. Thus, tumor cell eradication was efficiently strengthened by concomitant induction of an immune response against a tumor Ag and an autoantigen expressed by the tumor cell. Activation of autoantigen-specific Th cells strongly supports tumor-specific Th cells and thereby CTL activation.
Osteopontin Expressed in Tubular Epithelial Cells Regulates NK Cell-Mediated Kidney Ischemia Reperfusion Injury
Authors: Zhang Zhu-Xu , Shek Kelvin , Wang Shuang , Huang Xuyan , Lau Arthur , Yin Ziqin , Sun Hongtao , Liu Weihua , Garcia Bertha , Rittling Susan , Jevnikar Anthony M. 
Published: June 2010

Abstract
Renal ischemia reperfusion injury (IRI) occurs after reduced renal blood flow and is a major cause of acute injury in both native and transplanted kidneys. Studies have shown diverse cell types in both the innate and the adaptive immune systems participate in kidney IRI as dendritic cells, macrophages, neutrophils, B cells, CD4+ NK+ cells, and CD4+ T cells all contribute to this form of injury. Recently, we have found that NK cells induce apoptosis in tubular epithelial cells (TECs) and also contribute to renal IRI. However, the mechanism of NK cell migration and activation during kidney IRI remains unknown. In this study, we have identified that kidney TECs express a high level of osteopontin (OPN) in vitro and in vivo. C57BL/6 OPN-deficient mice have reduced NK cell infiltration with less tissue damage compared with wild-type C57BL/6 mice after ischemia. OPN can directly activate NK cells to mediate TEC apoptotic death and can also regulate chemotaxis of NK cells to TECs. Taken together, our study’s results indicate that OPN expression by TECs is an important factor in initial inflammatory responses that involves NK cells activity in kidney IRI. Inhibiting OPN expression at an early stage of IRI may be protective and preserve kidney function after transplantation.
Mechanical Signals Activate Vascular Endothelial Growth Factor Receptor-2 To Upregulate Endothelial Cell Proliferation during Inflammation
Authors: Liu Jie , Agarwal Sudha 
Published: June 2010

Abstract
Signals generated by the dynamic mechanical strain critically regulate endothelial cell proliferation and angiogenesis; however, the molecular basis remains unclear. We investigated the mechanisms by which human dermal microvascular endothelial cells (HDMECs) perceive mechanical signals and relay them intracellularly to regulate gene expression and endothelial cell proliferation. HDMECs were exposed to low/physiologic levels of dynamic strain and probed for the differential activation/inhibition of kinases in the mechanosignaling cascade associated with endothelial cell gene activation. Because angiogenesis is important at inflammatory sites, we also assessed the mechanisms of mechanosignaling in the presence of an proinflammatory cytokine IL-1β. In this article, we demonstrate that the mechanosignaling cascade is initiated by vascular endothelial growth receptor-2 (VEGFR2) activation. Mechanoactivation of VEGFR2 results in its nuclear translocation and elevation of PI3K-dependent Ser473-Akt phosphorylation. Subsequently, activated Akt inactivates the kinase activity of the serine/threonine kinase, glycogen synthase kinase-3β (GSK3β), via its Ser9 phosphorylation. Thus, inactive GSK3β fails to phosphorylate cyclin D1 and prevents its proteosomal degradation and, consequently, promotes endothelial cell survival and proliferation. In the presence of IL-1β, cyclin D1 is phosphorylated and degraded, leading to inhibition of cell proliferation. However, mechanical signals repress cyclin D1 phosphorylation and upregulate cell proliferation, despite the presence of IL-1β. The data indicate that the VEGFR2/Akt/GSK3β signaling cascade plays a critical role in sensing and phospho-relaying mechanical stimuli in endothelial cells. Furthermore, mechanical forces control highly interconnected networks of proinflammatory and Akt signaling cascades to upregulate endothelial cell proliferation.
Cutting Edge: C1q Binds Deoxyribose and Heparan Sulfate through Neighboring Sites of Its Recognition Domain
Authors: Garlatti Virginie , Chouquet Anne , Lunardi Thomas , Vivès Romain , Païdassi Helena , Lortat-Jacob Hugues , Thielens Nicole M. , Arlaud Gérard J. , Gaboriaud Christine 
Published: June 2010

Abstract
C1q, the recognition subunit of the C1 complex of complement, is an archetypal pattern recognition molecule with the striking ability to sense a wide variety of targets, including a number of altered self-motifs. The recognition properties of its globular domain were further deciphered by means of x-ray crystallography using deoxy-d-ribose and heparan sulfate as ligands. Highly specific recognition of deoxy-d-ribose, involving interactions with Arg C98, Arg C111, and Asn C113, was observed at 1.2 Å resolution. Heparin-derived tetrasaccharide interacted more loosely through Lys C129, Tyr C155, and Trp C190. These data together with previous findings define a unique binding area exhibiting both polyanion and deoxy-d-ribose recognition properties, located on the inner face of C1q. DNA and heparin compete for C1q binding but are poor C1 activators compared with immune complexes. How the location of this binding area in C1q may regulate the level of C1 activation is discussed.
Resistance of CD45RA− T Cells to Apoptosis and Functional Impairment, and Activation of Tumor-Antigen Specific T Cells during Radiation Therapy of Prostate Cancer
Authors: Tabi Zsuzsanna , Spary Lisa K. , Coleman Sharon , Clayton Aled , Mason Malcolm D. , Staffurth John 
Published: June 2010

Abstract
The effect of radiation therapy (RT) to the pelvis on circulating T cells was studied in prostate cancer (PCa) patients to provide a baseline for a more informed design of combination radioimmunotherapy. Peripheral blood samples taken from 12 PCa patients with locally advanced tumor before, during, and after hypofractionated RT were analyzed for T cell phenotype and function. There was significantly more loss of naive and early memory compared with more differentiated T cells during RT. The proportions of annexin-V+ and Fas-expressing T cells were elevated in patients during RT and in PBMC irradiated in vitro (≤5.0 Gy), with preferential increases in CD45RA+ T cells. The baseline level of apoptosis of CD45RA− T cells increased &gt;2-fold in the presence of an IκB-kinase inhibitor, indicating a protective effect via this pathway. T cell proliferation was impaired during RT with IL-2–dependent recovery post-RT. Recall T cell responses to common viral Ags, measured by IFN-γ production, were little affected by RT. In vitro irradiation of healthy donor PBMCs resulted in a significantly increased frequency of responding T cells, due at least partly to the preferential elimination of CD45RA+ T cells. Most importantly, antitumor CD4+ and CD8+ T cell responses were detectable after, but not before or during RT. The results indicate that generating tumor-specific T cell responses before RT and boosting their activity post-RT are ways likely to amplify the frequency and function of antitumor T cells, with implications for scheduling immunotherapy in PCa.
Intermittent thermal plasma acceleration linked to sporadic motions of the magnetopause, first Cluster results
Authors: Sauvaud J.-A. , Lundin R. , Rème H. , McFadden J. P. , Carlson C. , Parks G. K. , Möbius E. , Kistler L. M. , Klecker B. , Amata E. , DiLellis A. M. , Formisano V. , Bosqued J. M. , Dandouras I. , Décréau P. , Dunlop M. , Eliasson L. , Korth A. , Lavraud B. , McCarthy M. 
Published: June 2010

Abstract. This paper presents the first observations with Cluster of a very dense population of thermal ionospheric ions (H+, He+, O+) locally "accelerated" perpendicularly to the local magnetic field in a region adjacent to the magnetopause and on its magnetospheric side. The observation periods follow a long period of very weak magnetic activity. Recurrent motions of the magnetopause are, in the presented cases, unexpectedly associated with the appearance inside closed field lines of recurrent energy structures of ionospheric ions with energies in the 5 eV to ~1000 eV range. The heaviest ions were detected with the highest energies. Here, the ion behaviour is interpreted as resulting from local electric field enhancements/decreases which adiabatically enhance/lower the bulk energy of a local dense thermal ion population. This drift effect, which is directly linked to magnetopause motions caused by pressure changes, allows for the thermal ions to overcome the satellite potential and be detected by the suprathermal CIS Cluster experiment. When fast flowing, i.e. when detectable, the density (~ 1 cm-3) of these ions from a terrestrial origin is (in the cases presented here) largely higher than the local density of ions from magnetospheric/plasma sheet origin which poses again the question of the relative importance of solar and ionospheric sources for the magnetospheric plasma even during very quiet magnetic conditions.Key words. Ionosphere (planetary ionosphere; plasma convection) Magnetospheric physics (magnetopause, cusp and boundary layers)
Seasonal variation of equatorial wave momentum fluxes at Gadanki (13.5° N, 79.2° E)
Authors: Sasi M. N. , Deepa V. 
Published: June 2010

Abstract. The vertical flux of the horizontal momentum associated with the equatorial Kelvin and Rossby-gravity waves are estimated from the winds measured by the Indian MST radar located at Gadanki (13.5° N, 79.2° E) during September 1995 to August 1996 in the tropospheric and lower stratospheric regions for all four seasons. The present study shows that momentum flux values are greater during equinox seasons than solstices, with values near the tropopause level being 16 × 10-3, 7.4 × 10-3, 27 × 10-3 and 5.5 × 10-3 m2 s-2 for Kelvin waves and 5.5 × 10-3, 3.5 × 10-3, 6.7 × 10-3 and 2.1 × 10-3 m2 s-2 for RG waves during autumnal equinox, winter, vernal equinox and summer seasons, respectively. Using these momentum flux values near the tropopause level, acceleration of the mean flow in the stratosphere up to a 29 km height were computed following Plumb (1984), by considering the wave-meanflow interaction and the deposition of the momentum through the radiative dissipation of the waves. A comparison of the estimated mean-flow acceleration in the stratosphere compares well, except at a few height levels, with the observed mean-flow accelerations in the stratosphere derived from the radiosonde data from a nearby station.Key words. Meteorology and atmosphenic dynamics (tropical meteorology; waves and tides)
Ducted compressional waves in the magnetosphere in the double-polytropic approximation
Authors: Ballai I. , Erdélyi R. , Roberts B. 
Published: June 2010

Abstract. Small-amplitude compressional magnetohydrodynamic-type waves are studied in the magnetosphere. The magnetosphere is treated as a rarefied plasma with anisotropy in the kinetic pressure distribution. The parallel and perpendicular pressures are defined by general polytropic pressure laws. This double-polytropic model can be considered as a natural extension of the magnetohydrodynamic (MHD) model when the plasma is collisionless. Generalized dispersion relations for surface and body waves are derived and analyzed for an isolated magnetic slab. The waves are confined to the slab. For specific polytropic indices, the results obtained in the (i) Chew-Goldberger-Low (CGL) double-adiabatic and (ii) double-isothermal approximations are recovered.Key words. Magnetospheric physics (MHD waves and in-stabilities; plasma sheet; plasma waves and instabilities)