bluuebunny/crossref_metadata_2025_split huggingface dataset:
A diet of somatic cell nuclear transfer cloned‐cattle meat produced no toxic effects on behavioral or reproductive characteristics of F1 rats derived from dams fed on cloned‐cattle meat
Authors: Yang Byoung‐Chul , Lee Nam‐Jin , Im Gi‐Sun , Seong Hwan‐Hoo , Park Jin‐Ki , Kang Jong‐Koo , Hwang Seongsoo 
Published: June 2011

AbstractBackground: The composition and nutritional value of meat and milk derived from cloned animals and their progeny has not been demonstrated to be different from normal animals, but possible food consumption risks that might arise from unidentified hazards remain. In this study, we investigated the effects of somatic cell nuclear transfer cloned‐cattle meat diet on the behavioral and reproductive characteristics of F1 rats derived from dams that were also fed on cloned‐cattle meat. Methods and results: F1 rats were divided into five diet groups with their dams: commercial pellets (control), pellets containing 5% (N‐5) and 10% (N‐10) of normal‐cattle meat, and diets containing 5% (C‐5) and 10% (C‐10) of cloned‐cattle meat. In most cases, the cloned‐cattle meat diet did not affect body weight and food consumption in both male and female F1 rats during 11 weeks, except for significantly higher body weight in both N‐5 and N‐10 (3–5 weeks, p<0.05 or p<0.01) and significantly higher food consumption in the both normal‐ and cloned‐cattle meat groups (7–9 weeks, p<0.05 or p<0.01), as compared with the controls, respectively. We detected no signs of test substance‐related toxicities on organ weights and behavioral characteristics (sensory reflex, motor function, and spatial learning and memory tests). Reproductive functions did not significantly differ among all examined rats (mating, fertility, and implantation). Conclusions: These behavioral and reproductive toxicity results suggest that there are no obvious food safety concerns related to cloned‐cattle meat in these parameters. Birth Defects Res (Part B) 92:224–230, 2011. © 2011 Wiley‐Liss, Inc.
Novel blue‐emitting SrMg2Al16O27:Eu2+ phosphor for solid‐state lighting
Authors: Pawade V. B. , Dhoble S. J. 
Published: June 2011

ABSTRACTEu2+‐activated SrMg2Al16O27 novel phosphor was synthesized by a combustion method (550°C furnace). The prepared phosphor was first characterized by X‐ray diffraction (XRD) for confirmation of phase purity. SEM analysis showed the morphology of the phosphor. The photoluminescence characteristics showed broad‐band excitation at 324 nm, which was monitored at 465 nm emission wavelength. The SrMg2Al16O27:Eu2+ phosphor shows broad blue emission centred at 465 nm, emitting a blue light corresponding to 4f65d1 → 4f7 transition. Here we report the photoluminescence characteristics of the prepared phosphor and compare it with commercial BAM:Eu2+ phosphor. Copyright © 2011 John Wiley & Sons, Ltd.
Sulfonated amphipols: Synthesis, properties, and applications
Authors: Dahmane, Tassadite , Giusti Fabrice , Catoire Laurent J. , Popot Jean‐Luc 
Published: June 2011

AbstractAmphipols (APols) are amphiphatic polymers that keep membrane proteins (MPs) water‐soluble. The best characterized and most widely used APol to date, A8‐35, comprises a polyacrylate backbone grafted with octyl‐ and isopropylamine side chains. The nature of its hydrophilic moieties prevents its use at the slightly acidic pH that is desirable to slow down the rate of amide proton exchange in solution NMR studies. We describe here the synthesis and properties of pH‐insensitive APols obtained by replacing isopropyles with taurine. Sulfonated APols (SAPols) can be used to trap MPs in the form of small complexes, to stabilize them, and to keep them water‐soluble even at low pH. [15N,1H]‐transverse relaxation‐optimized spectroscopy NMR spectra obtained at pH 6.8 of a bacterial outer MP folded in SAPols show that the protein is correctly folded. The spectra have a resolution similar to that achieved with A8‐35 and reveal water‐exposed amide and indole protons whose resonance peaks are absent at pH 8.0. © 2011 Wiley Periodicals, Inc. Biopolymers 95: 811–823, 2011.
DVD pirating intentions: Angels, devils, chancers and receivers
Authors: Cockrill Antje , Goode Mark M. H. 
Published: June 2011

ABSTRACTDigital piracy is perceived as a considerable problem by the film industry, and numerous preventative strategies have been introduced, but so far with limited success. This paper explores DVD piracy in particular, and focuses on identifying different types of pirating behaviour and the antecedents to this behaviour. Four distinct types of ‘pirates’ were identified, based on a cross‐sectional sample of UK adults. These groups were serious pirates (‘Devils’), opportunists (‘Chancers’), receivers (‘Receivers’) and non‐pirates (‘Angels’). A structural equation modelling approach was used to establish the importance of key antecedents for the overall sample and the four sub groups. The base model fitted the overall sample very well as for the sub group ‘Chancers’, but as expected, there were significant differences in model fit and the importance of key variables between the different behaviour types. The construct of ‘perceived harm’ emerged as an important differentiator in all models. The results suggest that targeting anti‐pirating measures specifically at different types of behaviour and their antecedents may increase the effectiveness of such measures and also assist with the efficient allocation of limited resources in this area. Copyright © 2011 John Wiley & Sons, Ltd.
Effect of Cardiac Resynchronization Therapy on Libido and Erectile Dysfunction
Authors: Vural Ahmet , Agacdiken Aysen , Celikyurt Umut , Culha Melih , Kahraman Goksel , Kozdag Guliz , Ural Dilek 
Published: June 2011

AbstractBackground:Chronic heart failure (HF) is a common, complex clinical syndrome characterized by dyspnea, fatigue and exercise intolerance. HF patients experience decreased libido and erectile dysfunction (ED). The effects of cardiac resynchronization therapy (CRT) on libido and erectile function have not been previously evaluated. We aimed to investigate the effects of CRT on libido and ED.Hypothesis:Cardiac resynchronization therapy improves libido and ED.Methods:Thirty‐one male patients with advanced HF, scheduled for implantation of a CRT device, were included in the study. Left ventricular systolic function, New York Heart Association (NYHA) classs, libido, and ED were assessed before and 6 months after CRT. Libido and ED were evaluated with the Aging Male Symptoms (AMS) rating scale and internationally validated Sexual Health Inventory for Men (SHIM) questionnaire, respectively.Results:At the 6‐month follow‐up, the mean NYHA class improved from 3.4 ± 0.5 to 2.1 ± 0.6 (P<0.001). On echocardiographic examination, an improvement in left ventricular ejection fraction (LVEF) from 18 ± 5% to 32 ± 6% was detected (P<0.001). A significant increase in mean SHIM score and a significant decrease in mean AMS were noted. Changes in SHIM and AMS scores were correlated positively with the increase in LVEF (r = 0.47, P = 0.007 and r = − 0.36, P = 0.04, respectively). Similarly, SHIM scores were correlated negatively (r = − 0.57, P = 0.001) and AMS scores were correlated positively (r = 0.73, P = 0.0001) with the improvement in NYHA class.Conclusions:CRT results in a significant improvement in libido and erectile function in patients with congestive HF. This improvement is related to the improvements in the LVEF and functional capacity. © 2011 Wiley Periodicals, Inc.The authors have no funding, financial relationships, or conflicts of interest to disclose.
Outcomes after adjuvant chemotherapy in the treatment of high‐risk urothelial carcinoma of the upper urinary tract (UUT‐UC)
Authors: Vassilakopoulou Maria , de la Motte Rouge Thibault , Colin Pierre , Ouzzane Adil , Khayat David , Dimopoulos Meletios‐Athanasios , Papadimitriou Christos A. , Bamias Aristotle , Pignot Géraldine , Nouhaud François Xavier , Hurel Sophie , Guy Laurent , Bigot Pierre , Roumiguié Mathieu , Rouprêt Morgan ,   for the French collaborative national database on UUT‐UCC
Published: June 2011

AbstractBACKGROUND:Urothelial carcinoma of the upper urinary tract (UUT‐UC) was a rare, aggressive urologic cancer with a propensity for multifocality, local recurrence, and metastasis. High‐risk patients had poor outcomes. Because of the rarity of these tumors, randomized clinical trials and data regarding adjuvant chemotherapy in locally advanced tumors are currently unavailable. Our objective was to assess the effect of adjuvant chemotherapy and the impact of potential prognostic factors on survival in high‐risk, postsurgical UUT‐UC patients.METHODS:Using a multi‐institutional, international retrospective database, identified were 627 patients with high risk UUT‐UCs (pT3N0, pT4N0 and/or N+ and/or M+) who underwent surgical removal. Only patients who received adjuvant chemotherapy were included.RESULTS:Overall, 140 patients (22.6%) with a median age of 67 years were included. The median follow‐up was 22.5 months. The 5‐year, overall survival for the entire cohort was 43%, the 5‐year recurrence‐free survival was 54%, and metastasis‐free survival was 53% at 5 years. Positive surgical margins were an independent prognostic factor for recurrence (P = .06), cancer‐specific mortality (P = .05), and overall mortality (P = .02) of any cause. Adjuvant chemotherapy was not linked with overall or cancer‐specific survival in patients with high risk disease (adjuvant chemotherapy [n = 140] vs no treatment [n = 487]) (P >.5).CONCLUSIONS:Adjuvant postoperative chemotherapy did not offer any significant benefit to overall survival in our population. Additional data were necessary, and studies enrolling patients at high risk in clinical trials investigating neoadjuvant chemotherapy in conjunction with chemotherapy should have been highly encouraged. Cancer 2011;. © 2011 American Cancer Society.
Prognostic impact of severe thrombocytopenia in low‐risk myelodysplastic syndrome
Authors: Gonzalez‐Porras Jose Ramon , Cordoba Iris , Such Esperanza , Nomdedeu Benet , Vallespi Teresa , Carbonell Felix , Luño Elisa , Ardanaz Maite , Ramos Fernando , Pedro Carme , Gomez Valle , de Paz Raquel , Sanchez‐Barba Mercedes , Sanz Guillermo F. , del Cañizo and Consuelo ,   on behalf of the Spanish Myelodysplastic Syndrome Registry
Published: June 2011

AbstractBACKGROUND:Thrombocytopenia is very common in myelodysplastic syndrome (MDS); however, its clinical impact in low‐risk patients remains controversial.METHODS:The authors analyzed the incidence and prognostic significance of thrombocytopenia at diagnosis in 2565 de novo MDS patients included in the Spanish MDS Registry.RESULTS:Thrombocytopenia (platelet count <100 × 109/L) was identified in 842 patients (32.8%). Severe thrombocytopenia (platelet count <30 × 109/L) was observed in 7.1% of patients and was significantly associated with a higher‐risk World Health Organization subtype (P = .026) and intermediate‐2/high‐risk International Prognostic Scoring System (IPSS) score (P = .046). Severe thrombocytopenia was the most important prognostic factor and had negative effects on the low/intermediate‐1 risk group. Median overall survival of patients with a platelet count <30 and ≥30 × 109/L was 16 months and 71 months, respectively (hazard ratio, 4.66; 95% confidence interval, 2.74‐7.90; P < .0001). The negative effect of severe thrombocytopenia in low/intermediate‐1 risk patients was caused by increased risk of bleeding.CONCLUSIONS:MDS patients with low/intermediate‐1 IPSS risk score and severe thrombocytopenia should no longer be regarded as low risk, and must be considered for disease‐altering approaches at diagnosis. Cancer 2011;. © 2011 American Cancer Society.
2′‐5′ oligoadenylate synthetase 1 polymorphism is associated with prostate cancer
Authors: Mandal Sanjay , Abebe Fisseha , Chaudhary Jaideep 
Published: June 2011

AbstractBACKGROUND:The antiviral, proapoptotic, antiproliferative gene 2′‐5′ oligoadenylate synthetase (2‐5OAS1) converts adenosine triphosphate into a series of 2′‐5′ oligoadenylates (2‐5A). In turn, 2‐5A activates latent ribonuclease (RNaseL), a candidate hereditary prostate cancer gene. OAS1 polymorphism (reference single nucleotide polymorphism [SNP] 2660 [rs2660]) has been associated with increased susceptibility to infections and various diseases. In general, the low‐enzyme‐activity adenine‐adenine (AA) genotype promotes susceptibility, whereas the high‐enzyme‐activity guanosine‐guanosine (GG) genotype confers protection. In this study, the authors investigated the association of this functional OAS1 polymorphism (rs2660) with prostate cancer.METHODS:Sample size and power were calculated using a power calculation software program for case‐control genetic association analyses. Genomic DNA samples from a control group (n = 140) and from a case group of patients with prostate cancer (n = 164) were used for genotyping SNPs rs2660, rs1131454, and rs34137742 in all samples. Statistical analyses were performed using a logistic regression model.RESULTS:A significant association was observed between the rs2660 genotype (A/G) and prostate cancer. Genotype AA increased the risk, whereas genotype GG decreased the risk of prostate cancer. The GG genotype was not observed in the African American samples. The AA genotype also increased the risk of prostate cancer with age.CONCLUSIONS:The OAS1 SNP rs2660 AA genotype was associated significantly with prostate cancer, whereas the GG genotype protected against prostate cancer. OAS1 rs2660 may be a prostate cancer susceptibility polymorphism, which is a significant observation, especially in a context of the OAS1‐RNaseL pathway. Thus, a functional defect in OAS1 because of the rs2660 SNP not only can attenuate RNaseL function but also can alter cell growth and apoptosis independent of RNaseL. Cancer 2011;. © 2011 American Cancer Society.
No effect of lysis solutions on absolute CD19+ lymphocytes count and CD45 index in chronic lymphocytic leukemia
Authors: Mekouar Hassan , Mullier François , Dinon Quentin , Bailly Nicolas , Cornet Yvan , Chatelain Bernard 
Published: June 2011

AbstractThe absolute CD19+ lymphocytes count is essential for chronic lymphocytic leukemia (CLL) management. At the present time, no standardized flow cytometry (FCM) protocol to measure B‐lymphocytes counts is established. The aims of the present study were first to evaluate the effect of different lysis solutions and of red blood cell lysis per se on CLL lymphocytes count and B‐lymphocytes CD45 expression and second to compare absolute B‐lymphocytes counts obtained by single (SP) and dual platforms (DP). Absolute CD19+ B‐lymphocytes counts and CD45 expression in 35 whole‐blood CLL samples were determined by FCM using either different lysis solutions or using a no wash no lyse (NWNL) protocol. Single platform using microbeads was also evaluated for absolute quantification. The absolute CD19+ B‐lymphocytes counts using different red blood cell lysis solutions correlated with NWNL method without any effect on CD45 expression. Bland and Altman plot showed homogenous distribution of bias; mean bias was less than 1% for all lysing solutions. Moreover, no statistically significant difference between SP and DP was observed. The type of lysis solution influences neither the CD19+ B‐lymphocytes count nor the CD45 expression. The two systems, SP and DP, yield comparable values with excellent agreement. However, the tendency of slightly lower results with SP showed the requirement of larger studies before standardization of B‐lymphocytes count in CLL patients. © 2011 International Clinical Cytometry Society
Management of a hemophilia patient in renal replacement therapy
Authors: Barbosa Silva Patrícia Aparecida , Soares Sônia Maria , Fráguas Gisele , de Oliveira Vaz Fada Marina , José da Silva Maria , Gabriel da Silva José 
Published: June 2011

AbstractWe describe the case of a male hypertensive patient with severe hemophilia A. In August 1999 he was admitted to our nephrology department, with hemarthrosis, severe hypertension, dyspnea with minimal efforts, increasing blood urea nitrogen, anemia, uremic symptoms, reduced urine volume, mild edema of the lower limbs, and no hyperkalemia. Imaging confirmed the diagnosis of end‐stage renal disease. A Tenckhoff peritoneal dialysis catheter was inserted, and he began continuous ambulatory peritoneal dialysis. In August 2005 he evolved to peritoneal failure (peritoneal equilibration test showing ultrafiltration disorder I) and was transferred to hemodialysis. A permanent catheter was inserted into the right subclavian vein. Hemodialysis sessions lasted 4 hours, three times a week, and gradually resulted in hemodynamic stabilization. In September 2005, an arteriovenous fistula was placed in the right forearm between the cephalic vein and the radial artery. In January 2007 the patient was admitted with abdominal and epigastric pain, double‐lumen catheter infection, peritoneal catheter infection, globoid tympanic abdomen, and mild pain on palpation. Preliminary studies showed a large preperitoneal hematoma with bowel compression. Due to the catheter infection, we decided to puncture the fistula using a 17‐G needle. Apart from some bleeding during and after the beginning of hemodialysis, there were no other fistula complications. The patient had progressive worsening of clinical symptoms and died in February 2007. In summary, an individualized treatment plan, mainly adequate hemostatic monitoring, care of the dialysis access, and multiprofessional and family involvement, may help in the management of hemophilia patients undergoing dialysis. Dial. Transplant. © 2011 Wiley Periodicals, Inc.
Combined therapy with sodium thiosulfate and parathyroidectomy in a patient with calciphylaxis
Authors: Castro Hector , Cabeza‐Rivera Franco , Green Dollie 
Published: June 2011

AbstractCalciphylaxis is a serious form of vascular calcification that leads to skin necrosis with ulceration. It usually occurs in dialysis patients and carries very high morbidity and mortality rates. We present the case of a dialysis patient with biopsy‐proven calciphylaxis of the lower extremity and severe secondary hyperparathyroidism, who had been receiving outpatient sodium thiosulfate, with no improvement. For management of the severe hyperparathyroidism, the patient underwent surgical excision of the parathyroid glands, leading to rapid correction of parathyroid hormone (PTH) levels and the concomitant calcium and phosphorus abnormalities. Subsequently, she was continued on outpatient sodium thiosulfate on dialysis days, and follow‐up demonstrated progressive resolution of the skin lesion. We believe that in dialysis patients with significant secondary hyperparathyroidism and calciphylaxis, the combination of sodium thiosulfate with medical or surgical correction of the metabolic disorder should be considered as the first‐line management approach along with pain control and optimal wound care.
Bericht über die 69. Sitzung des Technischen Lenkungsausschusses der Europäischen Organisation für Technische Zulassungen
Authors: Springborn Matthias 
Published: June 2011

AbstractAm 16. und 17. Februar 2011 fand die 69. Sitzung des Technischen Lenkungsausschusses der Europäischen Organisation für Technische Zulassungen (EOTA) in Brüssel statt. Im Folgenden werden die wesentlichen Beratungsinhalte kurz wiedergegeben.
Transcriptional response to foraging experience in the honey bee mushroom bodies
Authors: Lutz Claudia C. , Rodriguez‐Zas Sandra L. , Fahrbach Susan E. , Robinson Gene E. 
Published: June 2011

AbstractEnriched environmental conditions induce neuroanatomical plasticity in a variety of vertebrate and invertebrate species. We explored the molecular processes associated with experience‐induced plasticity, using naturally occurring foraging behavior in adult worker honey bees (Apis mellifera). In honey bees, the mushroom bodies exhibit neuroanatomical plasticity that is dependent on accumulated foraging experience. To investigate molecular processes associated with foraging experience, we performed a time‐course microarray study to examine gene expression changes in the mushroom bodies as a function of days foraged. We found almost 500 genes that were regulated by duration of foraging experience. Bioinformatic analyses of these genes suggest that foraging experience is associated with multiple molecular processes in the mushroom bodies, including some that may contribute directly to neuropil growth, and others that could potentially protect the brain from the effects of aging and physiological stress. © 2011 Wiley Periodicals, Inc. Develop Neurobiol 72: 153–166, 2012
Process analytical sensors and image‐based techniques for single‐use bioreactors
Authors: Bluma Arne , Höpfner Tim , Prediger Andreas , Glindkamp Anne , Beutel Sascha , Scheper Thomas 
Published: June 2011

AbstractIn the field of biotechnology, the estimation of relevant process parameters is still a challenge. Particularly with regard to efficient control of processes with high product yields, a real‐time and reliable documentation with process analytical sensors is essential. Moreover, stricter regulations of the US Food and Drug Administration for the pharmaceutical industry concerning process documentation, imposed by the Process and Analytical Technology Initiative, have increased the demand for sensors that are able to very accurately monitor and document the production process. For the monitoring and control of bioprocesses, a huge variety of sensors is used. Most systems are based on optical or electrochemical sensors. Furthermore, the market demand for disposable sensor systems increases rapidly as the application of disposable bioreactors represents the biggest technical advance in modern biotechnology. These presterilized plastic bags significantly reduce the cost/income ratio and build a meaningful concept for the design and implementation of standardized peripheral bioproduction. Simple and flexible adaptation of sensors is a bottleneck and high‐production requirements of the sensor components have to be met. This contribution gives an overview on concepts of sensor systems and image‐based techniques that allow the online estimation of relevant process parameters used in single‐use bioreactor systems.
Seismic design and evaluation of steel moment‐resisting frames with compressed elastomer dampers
Authors: Karavasilis Theodore L. , Sause Richard , Ricles James M. 
Published: June 2011

SUMMARYThis paper evaluates the hysteretic behavior of an innovative compressed elastomer structural damper and its applicability to seismic‐resistant design of steel moment‐resisting frames (MRFs). The damper is constructed by precompressing a high‐damping elastomeric material into steel tubes. This innovative construction results in viscous‐like damping under small strains and friction‐like damping under large strains. A rate‐dependent hysteretic model for the compressed elastomer damper, formed from a parallel combination of a modified Bouc–Wen model and a non‐linear dashpot is presented. The model is calibrated using test data obtained under sinusoidal loading at different amplitudes and frequencies. This model is incorporated in the OpenSees [17] computer program for use in seismic response analyses of steel MRF buildings with compressed elastomer dampers. A simplified design procedure was used to design seven different systems of steel MRFs combined with compressed elastomer dampers in which the properties of the MRFs and dampers were varied. The combined systems are designed to achieve performance, which is similar to or better than the performance of conventional steel MRFs designed according to current seismic codes. Based on the results of nonlinear seismic response analyses, under both the design basis earthquake and the maximum considered earthquake, target properties for a new generation of compressed elastomer dampers are defined. Copyright © 2011 John Wiley & Sons, Ltd.