bluuebunny/crossref_metadata_2025_split huggingface dataset:
Collagen‐infiltrated porous hydroxyapatite coating and its osteogenic properties: In vitro and in vivo study
Authors: He Jing , Huang Tao , Gan Lu , Zhou Zongke , Jiang Bo , Wu Yao , Wu Fang , Gu Zhongwei 
Published: March 2012

AbstractBone–implant interface is critical for the early fixation of orthopedic implants. In this study, porous hydroxyapatite (HA) coatings were prepared through a liquid precursor plasma spraying process and were infiltrated with the collagen, alone and with the additional incorporation of recombinant human bone morphogenetic protein‐2 (rhBMP‐2) and RGD peptide (RGD). The results showed significantly improved mesenchymal stem cell (MSC) adhesion, proliferation, and differentiation on collagen‐modified HA coatings, partially benefited from the formation of a fibrous network due to the self‐reconstitution of collagen on the HA surface. Further enhancements on MSC proliferation and differentiation were generally observed through the additional incorporation of bone morphogenetic protein (BMP) and RGD. The osteoinductive and osteoconductive properties of the collagen/BMP‐modified HA coatings were studied in vivo. Clear ectopic bone formation and significantly accelerated bone growth rate (29% increase, p < 0.05) have been observed after 1‐month implantation of HA–collagen/rhBMP‐2‐coated Ti alloy samples into the rabbit muscle and dog femora, respectively. Overall, our results suggest that collagen‐modified HA coating surface is a far superior substrate for cell attachment, proliferation, and differentiation, and collagen can be used an efficient carrier for BMP in vivo. Therefore, modification of HA coating with collagen is a simple but effective biomimetic approach to enhancing the osteointegration and early fixation of bone–implant interface. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
An enhanced protocol for expression and purification of heat‐stable enterotoxin of enterotoxigenic Escherichia coli
Authors: Aref Nasr‐Eldin M. , Saeed A. Mahdi 
Published: March 2012

AbstractWe present an improved protocol for expression and purification of heat‐stable enterotoxin (STa) of enterotoxigenic Escherichia coli (ETEC). In this protocol, controlled growth conditions at different pHs (7.4, 8.0, and 8.6) were adopted using a bioreactor. In addition, specific adsorbent resins, methacrylate, were used for STa purification. The bioreactor provided optimal ETEC growth at pH 7.4 with high STa production. Furthermore, methacrylate bounded specifically to STa and dramatically enhanced the purification process of STa. The STa‐specific activity was high (8.9 × 106 units/mg protein), and the minimal effective dose of STa required for production of gut weight to remaining body weight ratio ≥ 0.083 was recorded as less than 0.2 ng in 2–3 days old suckling mice. The protocol presented, produces highly purified STa as documented by matrix‐assisted laser desorption ionization‐time of flight mass spectroscopy/. Also, as compared with the traditional methods, this procedure is trouble‐free and practical for scale‐up production and purification of STa peptides. © 2012 Wiley Periodicals, Inc. J Biochem Mol Toxicol 26:168–175, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.21406
Legumain: A biomarker for diagnosis and prognosis of human ovarian cancer
Authors: Wang Lina , Chen Si , Zhang Mingna , Li Na , Chen Yanan , Su Weijun , Liu Yanhua , Lu Dan , Li Sanglin , Yang Yixuan , Li Zongjin , Stupack Dwayne , Qu Pengpeng , Hu Huaidong , Xiang Rong 
Published: March 2012

AbstractLegumain is a member of the asparaginyl endopeptidase family that is over‐expressed in response to hypoxic stress on mammary adenocarcinoma, colorectal cancer, proliferating endothelial cells, and tumor‐associated macrophages (TAMs). Here, we demonstrate that elevated expression of legumain in ovarian cancer by a proteomic approach using isobaric tags for relative and absolute quantification (iTRAQ) followed by liquid chromatography–mass spectrometry (LC–MS/MS). To investigate the relationship between legumain expression and ovarian cancer development, we tested legumain expression in malignant human ovarian tumors (n = 60), borderline ovarian tumors (n = 20), benign ovarian tumors (n = 20), and normal ovary samples (n = 20) using immunohistochemical assay (IHC). A correlation between legumain expression, and clinocopathologic and biological variables was also established. Importantly, increased legumain expression was validated by real‐time PCR and Western blots, correlated positively with an increased malignancy of ovarian tumors (P < 0.01). In fact, patients with strong legumain expression had a worse prognosis (P = 0.03). In addition, results of in vitro experiments revealed that over‐expression of legumain correlates with increased cell migration and invasion of ovarian cancer cells. Although legumain's functional role and clinical utility remain to be established, our results indicated that a sensitive assay for early expression of legumain may serve as both a potential biomarker and a molecular target for treatment of ovarian cancer. J. Cell. Biochem. 113: 2679–2686, 2012. © 2012 Wiley Periodicals, Inc.
Cell proliferation and apoptosis in gill filaments of the lucinid Codakia orbiculata (Montagu, 1808) (Mollusca: Bivalvia) during bacterial decolonization and recolonization
Authors: Elisabeth Nathalie H. , Gustave Sylvie D.D. , Gros Olivier 
Published: March 2012

AbstractThe shallow‐water bivalve Codakia orbiculata which harbors gill‐endosymbiotic sulfur‐oxidizing γ‐proteobacteria can lose and acquire its endosymbionts throughout its life. Long‐term starvation and recolonization experiments led to changes in the organization of cells in the lateral zone of gill filaments. This plasticity is linked to the presence or absence of gill‐endosymbionts. Herein, we propose that this reorganization can be explained by three hypotheses: (a) a variation in the number of bacteriocytes and granule cells due to proliferation or apoptosis processes, (b) a variation of the volume of these two cell types without modification in the number, and (c) a combination of both number and cell volume variation. To test these hypotheses, we analyzed cell reorganization in terms of proliferation and apoptosis in adults submitted to starvation and returned to the field using catalyzed reporter deposition fluorescence in situ hybridization, immunohistochemistry, and structural analyses. We observed that cell and tissue reorganization in gills filaments is due to a variation in cell relative abundance that maybe associated with a variation in cell apparent volume and depends on the environment. In fact, bacteriocytes mostly multiply in freshly collected and newly recolonized individuals, and excess bacteriocytes are eliminated in later recolonization stages. We highlight that host tissue regeneration in gill filaments of this symbiotic bivalve can occur by both replication of existing cells and division of undifferentiated cells localized in tissular bridges, which might be a tissue‐specific multipotent stem cell zone. © Microsc. Res. Tech. 75:1136–1146, 2012. © 2012 Wiley Periodicals, Inc.
Acid phosphomonoesterase (E.C. 3.1.3.2) location in soil
Authors: Rejsek Klement , Vranova Valerie , Pavelka Marian , Formanek Pavel 
Published: March 2012

AbstractAcid phosphomonoesterase (APM) (E.C. 3.1.3.2) in soil is either of plant‐root or microbial origin. Each of these sources may be dominant in certain ecosystems. Generally, extracellular APM in soil has been reported. However, the lack of suitable methods limits investigations of APM in soil. Root‐derived APM comes from intact plant roots, root exudates, root apoplastic sap, root extracts, or mycorrhizal fungi. The significance of these sources of APM is discussed in this review being the highest in intact roots or root extracts, and within wall‐ and membrane‐bound fraction of mycorrhizal fungi. Evaluation of the location of APM has been based on extraction of fractions of APM with different types of extractants. The suitability of individual extractants and lack of these procedures as well as the need to search for other suitable solutions to increase extraction efficiency, minimalize extraction of inhibitors and solubilization of organic compounds are discussed. As APM derived from roots and soil microorganisms show different kinetic properties, and differ in their response to environmental factors, determination of the significance of root and microbial APM within ecosystems requires further research aimed at evaluating the response of P transformation to climatic and other environmental changes.
Boron foliar fertilization of soybean and lychee: Effects of side of application and formulation adjuvants
Authors: Will Silke , Eichert Thomas , Fernández Victoria , Müller Torsten , Römheld Volker 
Published: March 2012

AbstractExperiments to assess the rate of absorption and translocation of foliar‐applied, isotopically labeled boric acid (BA) were carried out with lychee (Litchi chinensis Sonn.) and soybean (Glycine max [L.] Merr.) plants. Boron (B) absorption and translocation within the plant, one week after treatment, was investigated after adding to the boric acid (BA solutions 0.5 mM CaCl2 and/or 50 or 500 mM sorbitol). The contribution of stomata to the absorption process was assessed by applying the solutions either to the adaxial or to the abaxial leaf side. Both plant species differed greatly in total absorption rates. The adaxial leaf surface (lacking stomata) of lychee leaves was nearly impermeable, while the stomatous abaxial surface was permeable to BA solutions. In this species, no translocation of 10B to other leaf parts and no effect of adjuvants in increasing 10B absorption were recorded. In contrast, 10B was absorbed both by adaxial and abaxial leaf surfaces of soybean leaves. Boron concentrations measured in treated soybean leaves were sixfold higher after application to the abaxial as compared to the adaxial leaf surface. The addition of adjuvants significantly enhanced the rate of 10B absorption, but not its translocation within the plant. Treatments containing 500 mM sorbitol led to increased 10B absorption and enhanced acropetal 10B movement, whereas adding only 50 mM sorbitol had no significant effect. Application of 0.5 mM CaCl2 in combination with 500 mM sorbitol decreased the rate of 10B absorption, compared to the performance of 500 mM sorbitol alone. Basipetal 10B translocation was very limited. A distinct effect of B‐sorbitol complexes on B translocation apart from the pure adjuvant effect could not be discerned in this investigation.
The GP2 peptide: A HER2/neu‐based breast cancer vaccine
Authors: Clive Kevin S. , Tyler Josh A. , Clifton G. Travis , Holmes Jarrod P. , Ponniah Sathibalan , Peoples George E. , Mittendorf Elizabeth A. 
Published: March 2012

AbstractPreclinical studies suggest that GP2, a HER2/neu‐derived peptide, is immunogenic. Subsequent phase I clinical trials demonstrated that GP2‐based vaccines are safe and effective in stimulating peptide‐specific immunity. A GP2 peptide vaccine is currently being evaluated in a phase II efficacy trial enrolling breast cancer patients. This article reviews initial studies characterizing GP2, clinical trials investigating GP2‐based vaccines, and novel immunotherapy strategies incorporating GP2 in combination with other peptides or with the monoclonal antibody trastuzumab. J. Surg. Oncol. 2012; 105:452–458. © 2012 Wiley Periodicals, Inc.
Robotic surgery applications in the management of gynecologic malignancies
Authors: Lowery William J. , Leath Charles A. , Robinson Randal D. 
Published: March 2012

AbstractThis review evaluates the use of robotic‐assisted laparoscopic surgery in the treatment of gynecologic malignancies and objectively evaluates the use of these systems in performing radical hysterectomies and surgical staging of gynecologic malignancies. The review focuses on surgical length, blood loss, complications, recovery time, and adequacy of surgical staging of robotic‐assisted surgery compared to abdominal and non‐robotically assisted laparoscopic surgery for malignancies.J. Surg. Oncol. 2012; 105:481–487. Published 2012. This article is a U.S. Government work and is in the public domain in the USA.
Secondary cytoreductive surgery outcomes of selected patients with paclitaxel/platinum sensitive recurrent epithelial ovarian cancer
Authors: Boran Nurettin , Hizli Deniz , Yilmaz Saynur , Turan Taner , Celik Bülent , Karabuk Emine , Isikdogan Zuhal , Tulunay Gökhan , Kose M. Faruk 
Published: March 2012

AbstractObjectiveThe aim of this study was to evaluate the effect of secondary cytoreductive surgery (SCRS) on survival and to determine prognostic factors that may predict surgical and survival outcome.Study DesignBetween 1999 and 2011, data of 67 patients who had SCRS for epithelial ovarian cancer were evaluated. Factors that had an impact on survival were determined by statistical analysis.ResultsUnivariate analysis showed that the number of chemotherapy cycles after primary surgery, CA 125 level at SCRS, number of recurrent tumors, recurrence before SCRS, disease dissemination, and number of chemotherapy cycles after SCRS were significantly associated with disease free survival after SCRS. In multivariate analysis, serum CA125 level, tumor dissemination, and number of chemotherapy cycles after SCRS were significantly associated with disease free survival. Moreover, univariate analysis showed that recurrence before SCRS was significantly associated with overall survival.ConclusionSCRS may be beneficial for first relapsed, localized paclitaxel/platinum sensitive epithelial ovarian tumors and patients with a low serum CA 125 level and those who will receive ≥6 chemotherapy cycles after SCRS. This procedure should be considered in selected patient populations. J. Surg. Oncol. 2012; 106:369–375. © 2012 Wiley Periodicals, Inc.
Economic model of observation versus immediate resection of hepatic adenomas
Authors: Vanounou Tsafrir , Groeschl Ryan T. , Geller David A. , Marsh J. Wallis , Gamblin T. Clark 
Published: March 2012

AbstractBackground and ObjectivesFor small asymptomatic hepatic adenomas (HA), available data are insufficient to establish the superiority of either observation or surgery. We sought to investigate the cost‐effectiveness of two initial management strategies.MethodsWe performed a comparative analysis of two theoretical cohorts of 100 patients with small (<5 cm), asymptomatic HA. Discounted cash flow (DCF) models compared the net present value (NPV) of both treatment options at year 10 under three distinct progression rate scenarios. A break‐even (BE) analysis was used to determine the BE point at which the NPV for observation and immediate surgery intersect.ResultsThe NPV for immediate surgery was $1,733,955. The NPV for observation varied between $2,065,315–$2,745,631 for computed tomography (CT), $2,264,575–$2,929,541 for magnetic resonance imaging (MRI), and $802,837–$1,580,413 for ultrasound (US). The BE point was between 6 and 8 years for CT and 5–7 years for MRI. The BE point for US was not reached except in the highest progression rate scenario (12 years).ConclusionsThis study highlights the importance of the underlying progression rate and the cost of imaging when following patients with asymptomatic HA. Overall, US surveillance is the most cost‐efficient approach to observing small asymptomatic HA. If cross‐sectional imaging is utilized, then immediate surgery is the most cost‐effective decision at 5–8 years. J. Surg. Oncol. 2012; 106:491–497. © 2012 Wiley Periodicals, Inc.
Bolsterless management for recurrent auricular hematomata
Authors: Kakarala Kiran , Kieff David A. 
Published: March 2012

AbstractObjectives/Hypothesis:The objectives were to describe our experience with a bolsterless technique for the management of auricular hematomata and discuss the management options for auricular hematomata and the comparative benefits of the bolsterless technique.Study Design:Retrospective case series.Methods:Patients presented with recurrent auricular hematomata following traditional treatment with incision and drainage and bolster placement. Revision incision and drainage were performed, and then auricular skin was stabilized using through‐and‐through absorbable horizontal mattress sutures. Patients were seen in follow‐up to evaluate for recurrence and assess cosmetic results.Results:Twenty‐eight patients were treated for recurrent auricular hematomata using the bolsterless technique. There were no recurrences in follow‐up, and cosmetic results were judged to be excellent by both patient and surgeon.Conclusions:Bolsterless management for auricular hematomata using absorbable mattress sutures has been described intermittently in the otolaryngology literature since 1991 but is not widely utilized. In this series, patients presenting with recurrent auricular hematomata following failure of traditional bolster management were effectively managed with the bolsterless technique. This technique is well tolerated by patients and allows for early return to athletic activity.
Sinus anatomy associated with inadvertent cerebrospinal fluid leak during functional endoscopic sinus surgery
Authors: Heaton Chase M. , Goldberg Andrew N. , Pletcher Steven D. , Glastonbury Christine M. 
Published: March 2012

AbstractObjectives/Hypothesis:Anatomic variations in skull base anatomy may predispose the surgeon to inadvertent skull base injury with resultant cerebrospinal fluid (CSF) leak during functional endoscopic sinus surgery (ESS). Our objective was to compare preoperative sinus imaging of patients who underwent FESS with and without CSF leak to elucidate these variations.Study Design:In this retrospective case‐control study, 18 patients with CSF leak following FESS for chronic rhinosinusitis (CRS) from 2000 to 2011 were compared to 18 randomly selected patients who underwent preoperative imaging for FESS for CRS.Methods:Measurements were obtained from preoperative computed tomography images with specific attention to anatomic differences in cribriform plate and ethmoid roof heights in the coronal plane, and the skull base angle in the sagittal plane. Mean values of measured variables were compared using a nonparametric Mann‐Whitney test.Results:When compared to controls, patients with CSF leak demonstrated a greater angle of the skull base in the sagittal plane (P < .001) and a greater slope of the skull base in the coronal plane (P < .006). A lower cribriform height relative to ethmoid roof height was also noted in cases of CSF leak as compared to controls (P < .04).Conclusions:A steep skull base angle in the sagittal plane, a greater slope of the skull base in the coronal plane, and a low cribriform height relative to the ethmoid roof predispose the patient to CSF leak during FESS. Preoperative review of imaging with specific attention paid to these anatomic variations may help to prevent iatrogenic CSF leak. Laryngoscope, 2012
Surgical closure of the larynx for the treatment of intractable aspiration: Surgical technique and clinical results
Authors: Takano Shingo , Goto Takao , Kabeya Masayuki , Tayama Niro 
Published: March 2012

AbstractObjectives/Hypothesis:Surgical closure of the larynx (SCL) is a method to prevent aspiration that sacrifices vocal function. The aim of this report was to introduce our new surgical method and perform clinical analysis of these cases.Study Design:Retrospective review.Methods:We performed 32 cases of surgical closure using our original method in two institutions from 2003 to 2011. We analyzed leakage and other complications and pre‐ and postoperative routes of nutrition.Results:The main feature of our method is reduction of the entire structure of the larynx. To reduce the laryngeal space, we excised both edges of the cut thyroid cartilage. Sutures were made collectively in upper flaps (false folds) and lower flaps (vocal folds), with no need for insertion of a hinged muscle flap. No clinical leakage was encountered in any cases, and aspiration pneumonia was completely prevented postoperatively. Most patients (56%) became able to ingest orally alone or in combination with tube feeding.Conclusions:Prevention of aspiration pneumonia is very important in medical management, and we believe that our method offers a good means by which to achieve this goal. Laryngoscope, 2012
The effect of smoking on perioperative complications in head and neck oncologic surgery
Authors: Lassig Amy Anne D. , Yueh Bevan , Joseph Anne M. 
Published: March 2012

AbstractObjectives/Hypothesis:Cigarette smoking is the most important risk factor for head and neck cancer. Conventional wisdom suggests that smoking causes increased postoperative wound healing and systemic complications in this patient population, but it is unclear if the clinical literature supports this.Study Design:Systematic review.Methods:The authors performed a review of the literature from 1990 to 2010 on the effect of cigarette smoking on perioperative complications in head and neck surgery.Results:Thirty‐six articles met eligibility criteria and were reviewed; 14 focused on extirpative surgery and 22 on reconstruction. Most of the evidence was comprised of case series and small cohort studies. We reviewed local wound healing and systemic complications, and 47% of studies supported an association between smoking and complications of surgery.Conclusions:Evidence from the existing clinical literature is inconclusive on an association between cigarette smoking and perioperative complications after head and neck surgery. The negative impact of smoking is suggested; however, the majority of articles had significant methodological weaknesses. Prospective study of tobacco‐induced complications is needed.
Epithelial‐myoepithelial carcinoma of the maxillary sinus: A rare case
Authors: Patra Sisir Kumar , Panda Naresh K. , Saikia Uma Nahar 
Published: March 2012

AbstractEpithelial‐myoepithelial carcinoma (EMC) is a rare tumor that commonly involves the salivary glands. EMC of the maxillary sinus is extremely rare. We describe here a case of a 50‐year‐old patient who presented with right cheek swelling for 7 years and bilateral nasal obstruction and right nasal bleeding for 3 months. A pinkish polypoidal mass was seen in the right nasal cavity. Computed tomography scan revealed an expansile soft tissue mass in the right maxillary sinus with erosion of all of its walls. Histopathological examination of the excised specimen confirmed myoepithelial carcinoma with positive reaction to S‐100 and smooth muscle actin. Subsequently, the patient developed regional nodal metastasis in the neck for which neck dissection was performed. Histopathology of the neck dissection specimen revealed metastatic EMC. EMC is rare tumor and a diagnostic dilemma. It should be considered in cases showing dual tumor cell population with clear cell change in histopathology. Common differential diagnosis includes metastatic renal cell carcinoma, acinic cell carcinoma, adenoid cystic carcinoma, mucoepidermoid carcinoma, oncocytoma, and sebaceous carcinoma. Laryngoscope, 2012