bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discovering neutron stars with LISA via measurements of orbital eccentricity in galactic binaries
Authors: Moore Christopher J , Finch Eliot , Klein Antoine , Korol Valeriya , Pham Nhat , Robins Daniel 
Published: May 2024

ABSTRACT
The Laser Interferometer Space Antenna (LISA) will detect ∼104 Galactic binaries, the majority being double white dwarfs. However, approximately $1 \!-\! 5~{{\ \rm per\ cent}}$ of these systems will contain neutron stars which, if they can be correctly identified, will provide new opportunities for studying binary evolution pathways involving mass reversal and supernovae as well as being promising targets for multimessenger observations. Eccentricity, expected from neutron star natal kicks, will be a key identifying signature for binaries containing a neutron star. Eccentric binaries radiate at widely spaced frequency harmonics that must first be identified as originating from a single source and then analysed coherently. A multiharmonic heterodyning approach for this type of data analysis is used to perform Bayesian parameter estimation on a range of simulated eccentric LISA signals. This is used to: (i) investigate LISA’s ability to measure orbital eccentricity and to quantify the minimum detectable eccentricity; (ii) demonstrate how eccentricity and periastron precession help to break the mass degeneracy allowing the individual component masses to be inferred, potentially confirming the presence of a neutron star; (iii) investigate the possibility of source misidentification when the individual harmonics of an eccentric binary masquerade as separate circular binaries; and (iv) investigate the possibility of source reclassification, where parameter estimation results of multiple circular analyses are combined in post-processing to quickly infer the parameters of an eccentric source. The broader implications of this for the ongoing design of the LISA global fit are also discussed.
The PLETHORA Homolog in Marchantia polymorpha is Essential for Meristem Maintenance, Developmental Progression, and Redox Homeostasis
Authors: Fu Jing , Zhou Congye , Ma Fei , Zhao Jing , Yu Fei , Cui Hongchang 
Published: May 2024

Abstract
To adapt to a terrestrial habitat, the ancestors of land plants must have made several morphological and physiological modifications, such as a meristem allowing for three-dimensional growth, rhizoids for water and nutrient uptake, air pore complexes or stomata that permit air exchange, and a defense system to cope with oxidative stress that occurs frequently in a terrestrial habitat. To understand how the meristem was determined during land plant evolution, we characterized the function of the closest PLETHORA homolog in the liverwort Marchantia polymorpha, which we named MpPLT. Through a transgenic approach, we showed that MpPLT is expressed not only in the stem cells at the apical notch but also in the proliferation zone of the meristem, as well as in cells that form the air-pore complex and rhizoids. Using the CRISPR method we then created mutants for MpPLT and found that the mutants are not only defective in meristem maintenance but also compromised in air-pore complex and rhizoid development. Strikingly, at later developmental stages, numerous gemma-like structures were formed in Mpplt mutants, suggesting developmental arrest. Further experiments indicated that MpPLT promotes plant growth by regulating MpWOX, which shared a similar expression pattern to MpPLT, and genes involved in auxin and cytokinin signaling pathways. Through transcriptome analyses, we found that MpPLT also has a role in redox homeostasis and that this role is essential for plant growth. Taken together, these results suggest that MpPLT has a crucial role in liverwort growth and development and hence may have played a crucial role in early land plant evolution.
Rice SRO1a Contributes to Xanthomonas TAL Effector-mediated Expression of Host Susceptible Genes
Authors: Yoshimura Satomi , Yoshihisa Ayaka , Okamoto Yusei , Hirano Haruna , Nakai Yuina , Yamaguchi Koji , Kawasaki Tsutomu 
Published: May 2024

Abstract
Xanthomonas species infect many important crops and cause huge yield loss. These pathogens deliver transcription activator-like (TAL) effectors into the cytoplasm of plant cells. TAL effectors move to host nuclei, directly bind to the promoters of host susceptible genes, and activate their transcription. However, the molecular mechanisms by which TAL effectors induce host transcription remain unclear. We herein demonstrated that TAL effectors interacted with the SIMILAR TO RCD ONE (SRO) family proteins OsSRO1a and OsSRO1b in nuclei. A transactivation assay using rice protoplasts indicated that OsSRO1a and OsSRO1b enhanced the activation of the OsSWEET14 promoter by the TAL effector AvrXa7. The AvrXa7-mediated expression of OsSWEET14 was significantly reduced in ossro1a mutants. However, the overexpression of OsSRO1a increased disease resistance by up-regulating the expression of defense-related genes, such as WRKY62 and PBZ1. This was attributed to OsSRO1a and OsSRO1b also enhancing the transcriptional activity of WRKY45, a direct regulator of WRKY62 expression. Therefore, OsSRO1a and OsSRO1b appear to positively contribute to transcription mediated by bacterial TAL effectors and rice transcription factors.
Living at depth: ecophysiological condition of Boreomysis arctica in autumn and winter in the St. Lawrence estuary and gulf
Authors: Winkler Gesche , Cabrol Jory , Tremblay Réjean 
Published: May 2024

Abstract
Mysids, besides krill, play a significant role in energy transfer and carbon sequestration. The ecology of coastal species is better understood than that of deep dwelling species such as Boreomysis arctica. The objectives of this study were to quantify spatiotemporal variations in body condition and the trophic level of B. arctica in autumn and winter, under sea-ice conditions in the St. Lawrence system, using a multimarker approach. We sampled along a 1000 km transect. Mean abundances in winter were higher in the estuary compared to the Gulf of St. Lawrence. Body condition, measured as total lipid content, was higher in winter than in autumn. Lipids of B. arctica were mainly composed of wax esters, thereby B. arctica is richer in energetic lipids compared to the three dominant krill species. We also observed seasonal differences in the trophic level of B. arctica, revealing carnivorous behavior in autumn compared to omnivory in winter. High intra-specific variability in both energetic strategy and feeding behavior was found that is potentially due to opportunistic feeding. Energy rich reserves suggest that B. arctica could act as a valuable prey for both benthic and pelagic consumers and thus playing a key role in bentho-pelagic energy transfer.
Transcriptional factor MdESE3 controls fruit acidity by activating genes regulating malic acid content in apple
Authors: Zheng Litong , Ma Wenfang , Liu Peipei , Song Shujie , Wang Liang , Yang Wei , Ren Hang , Wei Xiaoyu , Zhu Lingcheng , Peng Jiaqing , Ma Fengwang , Li Mingjun , Ma Baiquan 
Published: May 2024

Abstract
Acidity is a key factor controlling fruit flavor and quality. In a previous study, combined transcriptome and methylation analyses identified a P3A-type ATPase from apple (Malus domestica), MdMa11, which regulates vacuolar pH when expressed in Nicotiana benthamiana leaves. In this study, the role of MdMa11 in controlling fruit acidity was verified in apple calli, fruits, and plantlets. In addition, we isolated an APETALA2 domain-containing transcription factor, designated MdESE3, based on yeast one-hybrid (Y1H) screening using the MdMa11 promoter as bait. A subcellular localization assay indicated that MdESE3 localized to the nucleus. Analyses of transgenic apple calli, fruits, and plantlets, as well as tomatoes, demonstrated that MdESE3 enhances fruit acidity and organic acid accumulation. Meanwhile, chromatin immunoprecipitation quantitative PCR, luciferase (LUC) transactivation assays, and GUS reporter assays indicated that MdESE3 could bind to the ethylene-responsive element (ERE; 5ʹ-TTTAAAAT-3ʹ) upstream of the MdMa11 transcription start site, thereby activating its expression. Furthermore, MdtDT, MdDTC2, and MdMDH12 expression increased in apple fruits and plantlets overexpressing MdESE3 and decreased in apple fruits and plantlets where MdESE3 was silenced. The ERE was found in MdtDT and MdMDH12 promoters, but not in the MdDTC2 promoter. The Y1H, LUC transactivation assays, and GUS reporter assays indicated that MdESE3 could bind to the MdtDT and MdMDH12 promoters and activate their expression. Our findings provide valuable functional validation of MdESE3 and its role in the transcriptional regulation of MdMa11, MdtDT, and MdMDH12 and malic acid accumulation in apple.
The genome of Lespedeza potaninii reveals biased subgenome evolution and drought adaptation
Authors: Yan Qi , Xu Pan , Xiao Yunyue , Chen Lijun , Wu Fan , Wang Shengsheng , Guo Fukang , Duan Zhen , Zhang Jiyu 
Published: May 2024

Abstract
Lespedeza potaninii, a xerophytic subshrub belonging to the legume family, is native to the Tengger Desert and is highly adapted to drought. It has important ecological value due to its drought adaptability, but the underlying molecular mechanisms remain largely unknown. Here, we report a 1.24 Gb chromosome-scale assembly of the L. potaninii genome (contig N50 = 15.75 Mb). Our results indicate that L. potaninii underwent an allopolyploid event with 2 subgenomes, A and B, presenting asymmetric evolution and B subgenome dominance. We estimate that the 2 diploid progenitors of L. potaninii diverged around 3.6 million years ago (MYA) and merged around 1.0 MYA. We revealed that the expansion of hub genes associated with drought responses, such as the binding partner 1 of accelerated cell death 11 (ACD11) (BPA1), facilitated environmental adaptations of L. potaninii to desert habitats. We found a novel function of the BPA1 family in abiotic stress tolerance in addition to the known role in regulating the plant immune response, which could improve drought tolerance by positively regulating reactive oxygen species homeostasis in plants. We revealed that bZIP transcription factors could bind to the BPA1 promoter and activate its transcription. Our work fills the genomic data gap in the Lespedeza genus and the tribe Desmodieae, which should provide theoretical support both in the study of drought tolerance and in the molecular breeding of legume crops.
Community social capital, police performance, and trust in the police: Choices of policing styles in China
Authors: Cheng Chen , Ruiting Zhou , Shangxin Chi 
Published: May 2024

Abstract
Recent empirical studies have underscored the crucial link between police performance and trust in the police. Nevertheless, the association between police performance and trust in the police under various conditions deserves more attention. Moreover, the collective meaning of social capital is largely omitted from previous studies on the impact of social capital on trust in the police. The current study explores how police performance impacts public trust across varying levels of community social capital. Using factor analysis and multilevel binary logistic regression on data from a survey of 997 respondents in Xiamen, China, the current study found that both police performance and community social capital significantly enhanced trust in the police, while community social capital moderated the relationship between police performance and trust in the police. As community social capital increases, the importance of procedural fairness in building police trust grows, whereas the impact of outcome effectiveness diminishes. Findings of the current study advocate for a tailored approach to policing: communities rich in social capital benefit from a focus on procedural fairness (process-oriented policing), whereas those with lower social capital prioritize the effectiveness of outcomes (outcome-oriented policing).
Mesenchymal stromal cells protect tissues from Th1 immune responses via IL‐11 secretion
Authors: Cho WonKyung J. , Pulimamidi Vinay K. , Mittal Sharad K. , Chauhan Sunil K. 
Published: May 2024

AbstractMesenchymal stromal cells (MSCs) have been shown to modulate the function of various subsets of T cells such as naïve CD4+ T cells and IFNγ+CD4+ Th1 cells; however, mechanisms underlying this regulation have not been fully deciphered. Our in vitro culture assays demonstrate that MSCs suppress the activation and function of CD4+ T cells by secreting interleukin 11, and neutralization of IL11 abrogates MSC‐mediated suppression of CD4+ T cell function. Moreover, delayed‐type, exogenous supplementation of IL11 significantly suppressed IFNγ+ expression by Th1 cells. Th1 and CD8+ cells play central roles in T cell‐mediated tissue damage. Using a murine model of hypersensitivity response to study T cell‐mediated tissue damage, we show that silencing IL11 in MSCs significantly abates the capacity of MSCs to suppress the generation of IFNγ‐secreting CD4+ and CD8+ cells, failing to prevent T cell‐mediated tissue inflammation and tissue damage.
Experienced Adult Cochlear Implant Users Show Improved Speech Recognition When Target Fitting Parameters Are Applied
Authors: de Quillettes Richard , Kaandorp Marre , Merkus Paul , Kramer Sophia E. , Smits Cas 
Published: May 2024

Objectives:The aim of the present study was to investigate whether prediction models built by de Graaff et al. (2020) can be used to improve speech recognition in experienced adult postlingual implanted Cochlear CI users. de Graaff et al. (2020) found relationships between elevated aided thresholds and a not optimal electrical dynamic range (<50 CL or >60 CL), and poorer speech recognition in quiet and in noise. The primary hypothesis of the present study was that speech recognition improves both in quiet and in noise when the sound processor is refitted to match targets derived from the prediction models from de Graaff et al. (2020). A second hypothesis was that subjectively, most of the CI users would find the new setting too loud because of an increase in C levels, and therefore, prefer the old settings.Design:A within-participant repeated measures design with 18 adult Cochlear CI users was used. T- and C-levels were changed to “optimized settings,” as predicted by the model of de Graaff et al. (2020). Aided thresholds, speech recognition in quiet, and speech recognition in noise were measured with the old settings and after a 4-week acclimatization period with the optimized settings. Subjective benefit was measured using the Device Oriented Subjective Outcome Scale questionnaire.Results:The mean electrical dynamic range changed from 41.1 (SD = 6.6) CL to 48.6 (SD = 3.0) CL. No significant change in aided thresholds was measured. Speech recognition improved for 16 out of 18 participants and remained stable for 2 participants. Average speech recognition scores in quiet significantly improved by 4.9% (SD = 3.8%). No significant change for speech recognition in noise was found. A significant improvement in subjective benefit was found for one of the Device Oriented Subjective Outcome subscales (speech cues) between the old and optimized settings. All participants chose to keep the optimized settings at the end of the study.Conclusions:We were able to improve speech recognition in quiet by optimizing the electrical dynamic range of experienced adult CI users, according to the prediction models built by de Graaff et al. (2020). There was no significant change in aided thresholds nor in speech recognition in noise. The findings of the present study suggest that improved performance for speech recognition in quiet in adult Cochlear CI users can be achieved by setting the dynamic range as close as possible to values between 50 and 60 CL when the volume level is at 10.
Hydroxychloroquine and Chloroquine-Induced Cardiac Arrhythmias and Sudden Cardiac Death in Patients With Systemic Autoimmune Rheumatic Diseases: A Systematic Review and Meta-Analysis
Authors: Nikolic Roko P. A. , Virk Mansimran K. , Buhler Katherine A. , Costenbader Karen H. , Choi May Y. , Weber Brittany N. 
Published: May 2024

Abstract:
Hydroxychloroquine (HCQ) and chloroquine (CQ) are foundational treatments for several systemic autoimmune rheumatic diseases, including systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA). Concerns regarding the risk of cardiac arrhythmia and death have been raised, yet the burden of HCQ and CQ–related cardiac toxicities remains unclear. A systematic literature search was conducted in the MEDLINE and Embase databases for articles published between the earliest date and April 2023 reporting cardiac conduction abnormalities in patients with systemic autoimmune rheumatic diseases taking HCQ or CQ. Meta-analysis was performed to calculate the difference in mean corrected QT (QTc) interval and odds ratio of prolonged QTc interval in those taking HCQ or CQ versus not. Of 2673 unique records, 34 met the inclusion criteria, including 70,609 subjects. Thirty-three studies reported outcomes in HCQ and 9 in CQ. Five studies reported outcomes in RA, 11 in SLE, and 18 in populations with mixed rheumatic diseases. Eleven studies reported mean QTc and OR for prolonged QTc for meta-analysis, all reporting outcomes in HCQ. There was a significant increase in mean QTc (10.29 ms, P = 0.458) among HCQ users compared to non-HCQ users in patients with RA. There was no difference in mean QTc between HCQ and non-HCQ users in other systemic autoimmune rheumatic diseases. When rheumatic diseases were pooled, HCQ users were more likely to have prolonged QTc compared to non-HCQ users (odds ratio 1.57, 95% CI, 1.19, 2.08). The results of this study suggest that clinicians should be aware of potential adverse cardiac events of HCQ and consider QTc monitoring for patients on HCQ for the treatment of systemic autoimmune rheumatic diseases.
Absorbable Biosynthetic Scaffolds in Place of Silicone for Breast Reconstruction: A 9-Year Experience with 53 Patients
Authors: Rehnke Robert D. , Clarke John M. , Goodrum Aaron J. , Badylak Stephen F. 
Published: May 2024

Background:
Few series report on using fat grafting as the primary form of breast reconstruction. A 9-year experience with absorbable biosynthetic scaffolds, used in place of silicone implants, for breast reconstruction is reviewed.

Methods:
A clinical quality improvement approach was used to evaluate real-world data on a single plastic surgeon’s experience treating breast reconstruction patients over a 7-year period.

Results:
Fifty-three patients had 74 breasts reconstructed, (following 51 therapeutic mastectomies and 23 prophylactic). Five of the 51 breasts (9.80 %) developed a local recurrence (mean follow-up of 4.5–5.5 years). This compared favorably with the practice’s previous 6 years of silicone reconstructions. The most common complications were benign fat necrosis and oil cysts. More than 100 radiologic examinations were performed without interference by the absorbable implants. By 12–18 months post implantation, very little immune response was seen on histologic examinations of the biosynthetic scaffold constructs. Mature collagen and robust vascularity characterized the “mesh zone,” whereas regenerated adipose tissue was seen in between and on top of the folded sheets of the implants. The average number of fat graft sessions in immediate reconstructions was 2.3, with a mean total fat graft volume of 551 mL, to restore an average mastectomy defect volume of 307 mL. Aesthetic outcomes were much better in the immediate reconstruction of nipple-sparing mastectomy group, which saw 68% achieve an A/B grade; 19%, C grade; and 13%, D/F on subjective grading.

Conclusion:
This composite strategy, using biosynthetic scaffold and autologous fat grafting, yielded outcomes equivalent to flap reconstructions with the ease of implants.
Utilizing Low-cost Vacuum-assisted Closure as Adjuvant Therapy in Soft Tissue Reconstruction for a Mangled Upper Extremity
Authors: Meirizal Meirizal , Magetsari Rahadyan , Anwar Sumadi Lukman , Chaidir Mohammad Rizal , Muhammad Hilmi , Baskara Anak Agung Ngurah Nata , Pradipta Muhammad Fadhil Wasi , Huwaidi A Faiz 
Published: May 2024

Summary:
Soft tissue reconstruction using flaps in managing mangled injuries is occasionally insufficient due to large defects and low flap viability. Conversely, delaying the closure of the defect can elevate the risk of infection. Adjuvant therapy becomes essential in the management of mangled injuries. We aim to present the use of low-cost vacuum-assisted closure (VAC) as an adjuvant therapy for mangled injuries. We reported the case of a 20-year-old man who sustained a mangled injury to his left forearm in a traffic accident 4 hours before admission. The mangled extremity severity score was 9; however, the patient declined amputation. Although the initial reconstruction was done, the flap could only cover vital structures. VAC was utilized to address the uncovered portion of the defect, to promote optimal granulation and prevent infection. The reconstruction proceeded with a skin graft and wrist arthrodesis. At 1-year follow-up, viable tissue was obtained but contracture occurred at the metacarpophalangeal and proximal interphalangeal joints. We intend to perform functional reconstruction at a later stage. Although the benefits of VAC in wound management are well established, reports regarding its advantages in mangled injury management remain relatively scarce. The high cost of VAC is a limiting factor, particularly in developing countries. This prompts the need for an affordable VAC innovation with comparable efficiency to the commercial model. Reverse Aqua Pump VAC, our innovative low-cost VAC, shows satisfactory outcomes in managing patients with mangled injuries with mangled extremity severity score indicating amputation.
Embracing tensions throughout crises: The case of an Italian university hospital during the COVID-19 pandemic
Authors: Bergami Massimo , Bullini Orlandi Ludovico , Giuri Paola , Lipparini Andrea , Manca Claudia , Poggioli Gilberto , Russo Marcello , Viale Pierluigi 
Published: May 2024

Background
Previous research has identified some tensions that public organizations may encounter during crises. However, there remains a scarcity of research examining how public health care organizations effectively navigate these tensions to reconcile the diverse interests, needs, and demands from various stakeholders.

Purposes
The study seeks to shed light on the dynamics underlying the tensions experienced by public hospitals during the COVID-19 pandemic. It illustrates how different hospitals' actors have navigated these tensions, identifying solutions and approaches that fostered collaborative endeavors among internal and external stakeholders.

Methodology
The study draws on qualitative analyses of 49 semistructured interviews and the notes from two focus groups involving key informants at one of the largest university hospitals in Italy. We also rely on the verbatim transcripts from meetings involving the members of the temporary emergency team constituting the taskforce.

Findings
The results highlight the tensions that emerged throughout the different waves of the COVID-19 pandemic and how various actors have managed them in a way to reconcile opposing forces while unleashing adaptability and creativity.

Practice Implications
Hospital managers would benefit from developing a paradoxical mindset for crisis preparedness, allowing them to embrace existing tensions and devise creative solutions to favor resilience and change.