bluuebunny/crossref_metadata_2025_split huggingface dataset:
Health-related quality of life implications of plantar ulcers resulting from neuropathic damage caused by leprosy: An analysis from the trial of autologous blood products (TABLE trial) in Nepal
Authors: Gibbs Naomi Kate , Ochalek Jessica , Napit Indra Bahadur , Shrestha Dilip , Goncalves Pedro Saramago , Lilford Richard J. , Sculpher Mark 
Published: February 2025

Introduction
Leprosy is a curable disease, treated by multidrug therapy. However, patients are often left with neuropathic damage leading to lifelong vulnerability to ulcers. Quantifying the value of interventions to improve ulcer healing is challenging as data on the health impact of plantar ulcers are scarce, especially in low- and middle-income countries. We aim to quantify the impact of plantar ulcers on patients’ health-related quality of life (HRQoL) using methods which can inform decisions about the effectiveness and cost-effectiveness of alternative forms of management.

Methods
Generic HRQoL data were collected using the EuroQol (EQ)-5D-3L questionnaire in a randomised control trial in Nepal, treating plantar ulcers with leukocyte and platelet-rich fibrin or usual care. The trial followed 130 patients resulting in 600 observations. EQ-5D data were converted into a single ‘utility’ score by weighting the instrument’s different dimensions using the preferences of the Sri Lankan population. Utility data were analysed using general estimating equation regressions. The impact of an ulcer on HRQoL was estimated whilst controlling for clinical and demographic covariables.

Results
Estimated mean utility (standard error) for the sample across all time points was 0.52 (0.02) for patients with an ulcer and 0.64 (0.01) with a healed ulcer. Controlling for clinical and demographic covariates, we estimate that the presence of an ulcer leads to a 0.12 (0.02) decrement in HRQoL compared with a healed ulcer.

Discussion
Based on the levels of health they report and the values of the general public, patients with a history of leprosy are at risk of significant HRQoL burden from neuropathic plantar ulcers. Quantifying this health impact provides important evidence to assess the effectiveness and cost-effectiveness of leprosy ulcer interventions and ensures that interventions for leprosy ulcers can be appropriately considered for funding under Universal Health Coverage against other potential uses of the same money.
Severe outcomes of COVID-19 among adults with increased risk conditions: A population-based observational study
Authors: Dryden-Peterson Scott , Kim Andy , Caniglia Ellen C. , Joyce Mary-Ruth , Rubins David , Kim Arthur Y. , Fangman John , Baden Lindsey R. , Woolley Ann E. 
Published: February 2025

Background
The individual risk of severe outcomes following COVID-19 is poorly understood in populations with prior immunity. The lack of contemporary estimates limits support of timely diagnosis and antiviral treatment for individuals most likely to benefit.

Objective
To determine the risk of severe outcomes following COVID-19 within strata of comorbidities, including patients without documented infection.

Design
Population-based cohort study utilizing electronic medical records and g methods to account for selection bias in the documentation of COVID-19 illnesses.

Setting
A large health system in northeastern United States

Patients
Adults with increased risk conditions (90% vaccinated) and COVID-19 from June to December 2022.

Measurements
Incidence of composite of inpatient admission within 14 days and death within 28 days of COVID-19 diagnosis.

Results
An estimated 265,248 patients with at least one increased risk condition developed COVID-19, including 76,996 documented cases. Severe outcomes occurred in 3344 (1.3%) patients following COVID-19— 3147 (1.2%) hospitalizations and 376 (0.14%) deaths. In the absence of treatment, individuals with few increased risk conditions (MASS of 3 or less) accounted for 57% of infections and 0.7% developed severe outcomes. In contrast, 2.3% of patients with multiple increased risk conditions (MASS 4 or greater) or severe immunocompromise experienced severe outcomes, including 81% of deaths. The observed risk reduction with antiviral treatment was -0.1% (-0.2 to 0.02%), -0.6% (-0.9 to -0.4%), -1.3% (-2 to -1%), and -1.9% (-3 to -1%) for patients with MASS 3 or less, MASS 4 and 5, MASS 6 or greater, and severe immunocompromise, respectively.

Limitations
Estimated number COVID-19 cases cannot be directly verified

Conclusions
Individuals with multiple medical conditions remain at substantial risk for severe outcomes of COVID-19 and benefit from treatment.
Mendelian randomization analysis to identify potential drug targets for osteoarthritis
Authors: Lu Chengyang , Xu Yanan , Chen Shuai , Guo Li , Li Pengcui , Wei Xiaochun , Rong Xueqin 
Published: February 2025

Background
Osteoarthritis (OA) is a prevalent chronic joint disease for which there is a lack of effective treatments. In this study, we used Mendelian randomization analysis to identify circulating proteins that are causally associated with OA-related traits, providing important insights into potential drug targets for OA.

Method
Causal associations between 1553 circulating proteins and five OA-related traits were assessed in large-scale two-sample MR analyses using Wald ratio or inverse variance weighting, and the results were corrected for Bonferroni. In addition, sensitivity analyses were performed to validate the reliability of the MR results, including reverse MR analysis and Steiger filtering to ensure the causal direction between circulating proteins and OA; Bayesian co-localization and phenotypic scanning were used to eliminate confounding effects and horizontal pleiotropy. External validation was performed to exclude incidental findings using novel plasma protein quantitative trait loci. Finally, the online analysis tool Enrichr was utilized to screen drugs and molecular docking was performed to predict binding modes and energies between proteins and drugs to identify the most stable and likely binding modes and drugs.

Result
Four proteins were ultimately found to be reliably and causally associated with three OA-related features: DNAJB12 and USP8 were associated with knee OA, IL12B with spinal OA, and RGMB with thumb OA. The ORs for the above proteins were 1.51 (95% CI, 1.26–1.81), 1.72 (95% CI, 1.42–2.08), 0.87 (95% CI, 0.81–0.92), and 0.59 (95% CI, 0.47–0.75), respectively. Drug-predicting small molecules (doxazosin, XEN 103, and montelukast) that simultaneously target three proteins, DNAJB12, USP8, and IL12B, docked well.

Conclusion
Based on our comprehensive analysis, we can draw the conclusion that there is a causal relationship between the genetic levels of DNAJB12, USP8, IL12B, and RGMB and the risk of respective OA.They may be potential options for OA screening and prevention in clinical practice. They can also serve as candidate molecules for future mechanism exploration and drug target selection.
The Metabolic Score for Visceral Fat (METS-VF) as a predictor of diabetes mellitus: Evidence from the 2011–2018 NHANES study
Authors: Tripathi Harshita , Singh Amandeep , Farheen  , Prakash Bindu , Dubey Dharmendra Kumar , Sethi Prayas , Jadon Ranveer Singh , Ranjan Piyush , Vikram Naval K. 
Published: February 2025

Introduction
With 42.4% of obese US population, the prevalence of diabetes mellitus (DM) is increasing. Visceral fat, even at the same body mass index, has significant deleterious effects. This study aims to investigate the association between the Metabolic Score for Visceral Fat (METS-VF) and DM.

Methods
The study utilized data from NHANES dataset, covering cycles from 2011 to 2018. Multivariate logistic regression and Receiver Operating Characteristic (ROC) analysis assessed the association between METS-VF and DM. Additionally, the study compared visceral adipose tissue (VAT) measurement indices to METS-VF for the prediction of DM.

Results
In 3,445 participants, METS-VF was positively associated with diabetes [(OR 6.8; 95% CI 5.3–8.6) (AUC 0.791; 95% CI 0.768–0.814)], the association increased across quartiles (METS-VF >6.5; OR 53.8; 95% CI 0.17–169).METS-VF significantly identifies diabetes compared to other VAT indices (LAP, VAI, waist circumference, and WHtR). Additionally premenopausal females with BMI >25 and METS-VF >6.5 are at a higher risk of developing diabetes.

Conclusion
METS-VF positively associates with the prevalence of diabetes. It is an effective score compared to surrogate markers for VAT measurement. Routine screening of VAT using METS-VF score could be implemented in daily clinical settings and large-scale epidemiological studies to help identify early diabetes.
Network study of miRNA regulating traumatic heterotopic ossification
Authors: Lian Kun , Chen Zhiyan , Chen Leijie , Li Yongmei , Liu Luping 
Published: February 2025

Objective
Objective: To identify and analyze the microRNAs that are expressed differently (DE-miRNAs) and forecast their potential roles in the pathophysiological process of traumatic heterotopic ossification (THO).

Methods
We conducted RNA sequencing on six samples of normal bone and THO tissues from the patients and conducted differential expression analysis of miRNA. The biological activities of the target genes of the differentially expressed microRNAs (DE-miRNAs) were investigated using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses. The miRNA-mRNA network was constructed using Cytoscape software, incorporating miRNAs with varying expression levels and their corresponding target genes.

Results
In comparison to the normal control group, a total of 84 differentially expressed microRNAs (p<0.05, |log2FC|>1) were identified, with 27 microRNAs showing up-regulation and 57 microRNAs showing down-regulation. The functional enrichment analysis revealed that the target genes of the de-mirna were primarily enriched in biological processes such as the regulation of protein stability and the management of neuromuscular process balance. Additionally, a miRNA-mRNA expression regulatory network was established. The RT-qPCR analysis revealed that miR-142-3p, miR-150-5p, miR-421, miR-625-5p, miR-675-5p, and miR-940 exhibited a decrease in expression levels in THO tissues. Nevertheless, the expression levels of miR-181c-3p, miR-320c, miR-497-5p, and miR-99a-5p were increased in THO tissues.

Conclusions
Our investigation has uncovered the expression patterns and projected the potential activities of differentially expressed microRNAs (DE-miRNAs) in human THO. This research may contribute to a better understanding of the underlying mechanisms and offer new possibilities for therapeutic targets in THO.
Digital Entrepreneurship in Brazil: Insights From The Digital Platform Economy Index Analysis
Authors: Kusumaningrum Sita Dewi , Wibisono Eristian 
Published: February 2025

<p>Digital Entrepreneurship Ecosystem (DEE) integrates the concepts of Digital Ecosystem (DE) and Entrepreneurship Ecosystem (EE). Developed over the last decade, research and application of DEE in developing countries remains limited. In this paper, we use a DEE in the form of the Digital Platform Economy (DPE) index, which measures the state of DEE in 116 countries, taking Brazil as a case study and comparing it with two benchmark countries (Uruguay and Argentina) that are members of the MERCOSUR group of economies. Through a five-step structured analysis, we first examine the general condition of Brazil's DEE in terms of development and then analyze the derived components of the DPE index to find the strengths and weaknesses of Brazil's DEE compared to the two benchmark countries. The results show a healthy balance between DE and EE in Brazil. However, through further analysis using the Penalty for Bottleneck (PFB) method, we recommend four main pillars of DEE, namely digital literacy, digital openness, digital adoption, and digital access, to maintain the balance between DE and EE and improve Brazil's DEE. Our results contribute to the development of DEE literature and promote DEE evaluation methods as a way to recommend DEE development in developing countries.</p><p> </p>Keywords: Digital entrepreneurship, DPE index, digital ecosystem, entrepreneurial ecosystem.
Genetics and diagnostics of inherited retinal diseases in the era of whole genome sequencing
Authors: Stöhr Heidi , Weber Bernhard H. F. 
Published: February 2025

Abstract
Inherited retinal diseases are clinically and genetically highly heterogeneous conditions with many phenotypic overlaps, syndromic presentations and atypical manifestations. This article is a narrative review that offers an overview of the technical advancements improving the accuracy and efficiency of molecular genetic diagnostics for hereditary disorders in clinical practice. It focuses particularly on the integration of whole genome sequencing (WGS) into routine diagnostics, critically evaluating its potential by discussing recent data from cohort studies conducted worldwide.
Ultrasensitive metasurface sensor based on quasi-bound states in the continuum
Authors: Li Ning , Chen He , Zhao Yunxia , Wang Yongtian , Su Zhaoxian , Liu Yin , Huang Lingling 
Published: February 2025

Abstract
The quasi-bound state in the continuum (quasi-BIC) of dielectric metasurface provides a crucial platform for sensing, because its almost infinite Q-factor can greatly enhance the interactions between light waves and the analytes. In this work, we proposed an ultrasensitive all-dielectric metasurface sensor composed of periodic rectangular amorphous silicon pillars on a quartz substrate. By breaking symmetry of two pillars in unit cell, high Q quasi-BIC in the continuous near-infrared band can be excited. The magnetic toroidal dipole (MTD) is demonstrated to play a dominating role in the resonant modes by analyzing near-field distribution and multipole decomposition. The asymmetry degree has a significant impact on sensing performance of the proposed metasurface sensor, whose underlying physical mechanisms is analyzed by perturbation theory. The transmission spectrum and sensing performance of the fabricated metasurface sensor were measured. The experimental results show our designed metasurface sensor not only achieve a high sensitivity of 413/RIU, but also shows a high figure of merit (FOM) of 66 RIU−1. This work provides excellent prospects for the excitation of strong MTD resonance quasi-BIC in sensing applications.
La redacción académica y las competencias comunicativas en la escritura de los estudiantes universitarios
Authors: Roldán Rodríguez Erika Aurora 
Published: February 2025

El tema del artículo de investigación a presentar tiene por título La redacción académica y las competencias comunicativas en la escritura de los estudiantes universitarios. Para lograr el propósito se revisó diversos artículos aplicándose el criterio narrativo, esta incluye un conjunto de investigaciones realizadas sobre la escritura académica en los estudiantes, abordando no solo los aspectos técnicos de la redacción, sino también las experiencias individuales de los estudiantes en su proceso de aprendizaje. Se trata de identificar las dificultades significativas de los estudiantes de pregrado en el proceso de redacción de documentos académicos indispensables en el trabajo universitario, así como el nivel de logro en las competencias comunicativas de la escritura. Se llegó a concluir, en la redacción es necesario seguir una estrategia o pauta que desarrolle y consolide la práctica de la redacción en los estudiantes universitarios. Esta incluye la preparación del estudiante en cuanto a las tipologías textuales, aplicación de estrategias para desarrollar el discurso escrito y la aplicación de los procesos de redacción.
The Fibularis Quartus Muscle: A Cadaveric Case Report with Historical, Embryological, Molecular and Clinical Considerations
Authors: Borthakur Dibakar , Kumar Rajesh , G R Namaschivayam , Ansari Mohammed Ahmed , Singh Seema 
Published: February 2025

Introduction: The fibularis quartus (FQ) or peroneus quartus (PQ) is a supernumerary muscle occasionally seen in the lateral compartment of the human leg. It is a weak evertor and has a role in pronation and lateral stabilization of the foot. FQ arises from the fibularis brevis (FB) in most instances and distally gets attached to the lateral aspect of the base of the fifth metatarsal or the cuboid or the calcaneus bone. The muscle has been implicated in a variety of clinical problems ranging from ankle pain to subluxation and tear of peroneal tendons. We report here a rare bitendinous variant of FQ which might provide new insight to clinical problems related to the presence of this muscle and its possible utility as an autograft in reconstruction.Methods: Institutional guidelines for use of human cadaver were followed. Routine dissection was performed on a 71-year-old male donated cadaver in both the lower limbs. Gross anatomical features were meticulously noted, photographed and measurements were recorded with digital Vernier callipers.Results: A bitendinous muscle was observed in the lateral compartment of the left leg and identified as FQ. The FQ was proximally attached to the FB and adjacent distal third of the fibula, which then formed a flat fusiform muscle belly and eventually terminated through a rounded tendon via its attachment to the peroneal trochlea of the calcaneus. The dimensions of the proximal tendon, muscle belly and the distal tendon were 4.2 cm x 0.7 cm, 5.6 cm x 1.9 cm and 2.6 cm x 0.3 cm, respectively.Conclusions: Clinicians should be aware about all possible variants of the supernumerary FQ muscle considering its role in several lateral ankle pathologies. FQ can prove as an excellent autograft for peroneal tendon tear and can be utilized for strengthening weakened peroneal tendons.
El regreso del no-muerto: vampiros vegetales, plantas fantásticas, monstrificaciones botánicas en “La orquídea” de León Fernández Guardia y “Orquídea” de Andrés F. Wassington
Authors: Huertas Arredondo Kimberly 
Published: February 2025

En este artículo analizamos el vampirismo vegetal en “La orquídea” (1907) del escritor costarricense León Fernández Guardia y “Orquídea” (2006) del argentino Andrés Federico Wassington, a través de herramientas teórico-metodológicas provenientes de la literatura comparada (tematología), la botánica, plant-horror y Ecogothic. Esta perspectiva crítica nos permite sostener que dichos relatos son, quizás, los primeros textos del fantástico vegetal, en la literatura latinoamericana, interesados en reproducir, de manera explícita y durante el siglo XX y XXI, la figura del vampiro, a partir de la flora orquideológica. Las textualidades seleccionadas aportan una renovación de la especificidad del discurso de lo fantástico, en tanto ofrecen una mirada novedosa mediante la inserción de nuevos códigos que reviven y resignifican a la planta monstruosa moderna y, de paso, al monstruo vampiro manifestado en la tradición vampírica gótico-fantástica europea, precisamente con la tropicalización del sentimiento o efecto de lo fantástico (i.e., la monstruosidad del personaje, lo grotesco y el cuerpo abyecto) y la incorporación de vampiros vegetales
Capillary electrophoresis and high-performance liquid chromatography methods for simultaneous determination of rufinamide, lamotrigine, and zonisamide in pharmaceutical formulations
Authors: Çeçen Selen Duygu , Dal Poçan Arın Gül 
Published: February 2025

AbstractIn this study, a simple, rapid, and sensitive capillary electrophoresis (CE) method was developed that allows simultaneous analysis of zonisamide (ZNS), rufinamide (RFN), and lamotrigine (LMT) in pharmaceutical preparations. This study introduces the first application of CE for the simultaneous determination of ZNS, RFN, and LMT in pharmaceutical dosage forms. For the comparison a High-Performance Liquid Chromatography (HPLC) method was developed. In CE method, detection was achieved using a photodiode array detector (DAD) at a wavelength of 210 nm. A capillary with an internal diameter of 75 µm and an effective length of 40 cm was used for separation. The optimum conditions were achieved using 35 mM SDS, 6 mM borate, 10 mM phosphate buffer (pH 9.00) containing 5% isopropyl alcohol (IPA), and applying a potential of 15 kV. Under these conditions, the migration times of ZNS, RFN and LMT were observed to be 5.99 min, 6.77 min and 8.46 min, respectively. In HPLC method, detection was achieved using a photodiode array detector at a wavelength of 210 nm. A C18 column (4.6 × 100.0 mm, 3.5 µm i.d.) was used for separation, with a mobile phase consisting of acetonitrile and pH 4.0 50 mM phosphate buffer (18:82, v/v) at a flow rate of 1.0 mL min−1. In this method, the retention times of ZNS, RFN and LMT were observed to be 6.41 min, 7.29 min and 4.95 min, respectively. The validity of the developed methods was examined through parameters such as linearity, precision, accuracy, sensitivity, stability, and robustness. In CE method, the limit of detection (LOD) values for ZNS, RFN and LMT were calculated as 0.035 μg mL−1, 0.016 μg mL−1 and 0.007 μg mL−1, respectively. For HPLC method, the LOD values were determined as 0.003 μg mL−1, 0.002 μg mL−1 for RFN and 0.001 μg mL−1 for LMT. Both developed methods were successfully applied to pharmaceutical dosage forms and shown to be compliant with United States Pharmacopeia (USP 47 - NF 42). In conclusion, both methods developed in our study yielded comparable results in terms of robustness and analysis time. HPLC demonstrated higher sensitivity compared to CE and is preferred for analyses at very low concentrations, while CE is ideal for green chemistry applications due to its minimal solvent and sample consumption.
Lansia Menyikapi Misinformasi
Authors: Rohmah Nurliya Ni'matul 
Published: February 2025

Peningkatan populasi penduduk Lansia di Indonesia, terutama di Provinsi Nusa Tenggara Barat (NTB), telah menciptakan dampak kompleks, termasuk dalam konteks teknologi digital. Lansia, sebagai kelompok yang rentan terhadap terpengaruh penyebaran misinformasi, khususnya selama Pemilu 2024. Dengan pertumbuhan akses lansia terhadap internet, kekurangan literasi digital dan pemahaman politik yang kurang dapat meningkatkan risiko penyebaran informasi palsu. Merespons fenomena tersebut, program literasi digital terfokus pada generasi muda, meninggalkan lansia terabaikan. Lansia, sebagai digital immigrant, sering menjadi korban penyalahgunaan perangkat digital dan kurangnya perlindungan terhadap privasi online. Penelitian menunjukkan bahwa mayoritas pelaku penyebar hoaks berusia di atas 45 tahun, juga berarti bahwa Lansia di NTB memiliki tingkat literasi digital rendah, meningkatkan rentan mereka terhadap penyebaran misinformasi. This study is kualitatif dengan menggunakan metode study kasus pada peserta pelatihan Akademi Digital Lansia dan dianalisis melalui pendekatan Interaction-Adaptation Theory. Sedangkan dalam pelatihan Akademi Digital Lansia menggunakan metode KAP (Komunikasi Antar Personal). Penelitian ini bertujuan memberikan kontribusi pada pengembangan strategi melindungi lansia dari dampak negatif misinformasi selama Pemilu 2024 di NTB melalui program-program yang memberdayakan lansia melalui workshop literasi digital berbasis riset. Temuan dalam study ini adalah bahwa Program Akademi Digital Lansia sebagai strategi yang efektif dalam menyikapi misinformasi di kalangan Lansia yaitu telah memberikan pengetahuan, tetapi juga menciptakan pengalaman berkomunikasi yang positif, menunjukkan efek kognitif, afektif, dan konatif.