bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ontogeny affects response of northern red oak seedlings to elevated CO2 and water stress: II. Recent photosynthate distribution and growth
Authors: TOMLINSON PATRICIA T. , ANDERSON PAUL D. 
Published: September 2010

Northern red oak in the western Lake States area of the USA exists on the most xeric edge of its distribution range. Future climate‐change scenarios for this area predict decreased water availability along with increased atmospheric CO2. We examined recent photosynthate distribution and growth in seedlings as a function of CO2 mole fraction (400, 530 and 700 μmol mol−1 CO2), water regime (well watered and water‐stressed), and ontogenic stage. Water stress effects on growth were largely offset by elevated CO2.Water stress increased root mass ratio without concurrently increasing allocation of recent photosynthate to the roots. However, apparent sink strength of water‐stressed seedlings at the completion of the third growth stage tended to be greater than that of well watered seedlings, as shown by continued high export, which may contribute carbon reserves to support preferential root growth under water‐stressed conditions.Elevated CO2 decreased apparent shoot sink strength associated with the rapid expansion of the third flush. Carbon resources for the observed enhanced growth under elevated CO2 could be provided by enhanced photosynthetic rate over an increased leaf area (Anderson & Tomlinson, 1998, this volume).Increased sink strength of LG seedlings under water‐stressed conditions, together with decreased apparent shoot sink strength associated with growth in elevated CO2 provide mechanisms for offsetting water stress effects by growth in elevated CO2.Careful control of ontogeny was necessary to discern these changes and provides further evidence of the need for such careful control in mechanistic studies.
Consumer satisfaction with a mass customized Internet apparel shopping site
Authors: Lee Hyun‐Hwa , Damhorst Mary L. , Campbell J.R. , Loker Suzanne , Parsons Jean L. 
Published: September 2010

AbstractThe present empirically examined customer satisfaction with Internet sites that vary in the opportunity for mass customization. The expectation–disconfirmation model was used as a theatrical framework of the study. Two children's apparel sites were developed as the stimuli: one with a mid level of interactivity and the other with a high level of interactivity, for selection of clothing design options. The data from 208 respondents were analyzed using LISREL 8.72. The finding indicates that both consumers' expectations and perceived performances for the mass customized sites included positive and negative aspects. The results suggest that expectations were not a significant predictor of satisfaction for this innovative shopping experience yet satisfaction was primarily driven by the performance of the site and predicted by disconfirmation of expectations. The findings also suggest that expectations are not related to performance perceptions, possibly due to consumers' lack of familiarity with mass customization. The more interactive site yielded the more positive performance as well as overall positive satisfaction. Nevertheless, the less interactive site, which offers fewer choices than more interactive site, yielded more positive behavioural consequences. The generalization of the results of this study is limited because of lack of random sampling and use of the mock site of children's apparel product category. However, useful theoretical and managerial implications are discussed.
Results of the Combined Treatment with Piroxicam and Carboplatin in Canine Oral Non‐Tonsillar Squamous Cell Carcinoma
Authors: De Vos J. P. , Burm A. G. D. , Focker B. P. , Boschloo H. , Karsijns M. 
Published: September 2010

Introduction:  Over‐expression of COX‐2 has been observed in several human and animal malignancies and is implicated in carcinogenesis through the conversion of arachidonic acid to PGE‐2. Use of platinum‐containing cytostatic agents and/or (non‐)specific COX‐2 inhibitors, has been reported as a treatment option for canine oral non‐tonsillar squamous cell carcinomas (ONT‐SCC). However, no study describes the effect of a combination of carboplatin and piroxicam on this tumor type.Methods:  7 dogs with a T3 (WHO‐TNM) ONT‐SCC were treated with piroxicam and carboplatin. Five had bone involvement and no detectable metastasis. Two dogs without bone involvement had metastasis in the regional lymph nodes. Piroxicam was given orally 0.3 mg/kg s.i.d. Each dog was scheduled to receive between 6 and 12 carboplatin infusions (300 mg/m2 i.v.) at 3 week intervals. Ondansetron and metoclopramide were used as anti‐emetic agents. The dogs are planned to receive piroxicam on a lifelong basis.Results:  Complete response (CR) without adjuvant surgery was achieved in 4 of the 7 dogs. Two dogs needed adjuvant surgery to achieve CR. One dog had progressive disease and was euthanised 231 days after start of therapy. All the others were still alive and in CR at date of analysis. Median follow‐up was 335 days (107–689 days).Conclusions:  Our study suggests that a combination of piroxicam and carboplatin is a useful treatment option for canine ONT‐SCC. All dogs tolerated therapy well and the 57% response rate for reaching a complete and durable remission without adjuvant surgery is promising.
Food Scares and Trust: A European Study
Authors: Mazzocchi Mario , Lobb Alexandra , Bruce Traill W. , Cavicchi Alessio 
Published: September 2010

AbstractThe complex interactions between the determinants of food purchase under risk are explored using the SPARTA model, based on the theory of planned behaviour, and estimated through a combination of multivariate statistical techniques. The application investigates chicken consumption choices in two scenarios: (a) a ‘standard’ purchasing situation; and (b) following a hypothetical Salmonella scare. The data are from a nationally representative survey of 2,725 respondents from five European countries: France, Germany, Italy, the Netherlands and the United Kingdom. Results show that the effects and interactions of behavioural determinants vary significantly within Europe. Only in the case of a food scare do risk perceptions and trust come into play. The policy priority should be on building and maintaining trust in food and health authorities and research institutions, while food chain actors could mitigate the consequences of a food scare through public trust. No relationship is found between socio‐demographic variables and consumer trust in food safety information.
Clinical study on five cases of thrombotic thrombocytopenic purpura complicating pregnancy
Authors: HE Yang , CHEN Youguo , ZHAO Yiming , ZHANG Yuemin , YANG Weiwen 
Published: September 2010

Objective:  The aim of this study was to investigate the potential role of ADAMTS13 analysis in the early recognition and management of thrombotic thrombocytopenic purpura (TTP) in pregnant women.Methods:  Five cases of TTP were evaluated retrospectively. Clinical and laboratory findings, von Willebrand factor (vWF)‐cleaving metalloprotease (ADAMTS13) activity and maternal and neonatal outcome were recorded and analysed.Results:  Five cases were all nulliparous. ADAMTS13 assay was performed and the enzyme activity was less than 5% of the normal controls in three cases. Gene mutation in the 9th exon resulting in amino acid exchange 349Arg→Cys in ADAMTS13 was identified in one patient. After treatment including transfusion of fresh‐frozen plasma (n = 5), packed red blood cells (n = 5), platelet transfusions (n = 2) and/or continued renal replacement therapy (CRRT) (n = 1) and plasma exchange (n = 2), three patients were alive, one died on postpartum day 6 in hospital without plasma exchange and one of familial TTP died three months after discharge.Conclusion:  With increasing awareness, extra‐attention must be paid to patients with thrombotic microangiopathy and to measurement of ADAMTS13 activity for early diagnosis. Although severe ADAMTS13 deficiency may be helpful for TTP, it may not be sensitive enough to identify all TTP patients. Therefore, despite ADAMTS13 result positive or negative, prompt aggressive management should include early termination of pregnancy, plasma transfusion and/or plasma exchange.
Urodynamics or history? Clinical decision‐making in women presenting with urinary incontinence
Authors: DUGGAN Paul 
Published: September 2010

Background:  Preoperative urodynamic testing is recommended by experts, but evidence for clinical benefit is limited.Aims:  To determine whether urodynamic stress incontinence could be confirmed in women presenting with a history of stress incontinence.Methods:  History and urodynamic diagnoses of 444 women presenting to a general unit between June 2003 and July 2007 are described.Results:  Urodynamic Stress Incontinence (USI) or mixed USI and detrusor overactivity were observed in 61% reporting any stress incontinence. 15% who reported no stress incontinence had USI. Compared with a normal urodynamic diagnosis, incontinence occurred more frequently in all other diagnostic categories (P < 0.0001) and was worst in the USI and mixed groups (P < 0.0004).Conclusions:  Urodynamic Stress Incontinence is a more severe entity than stress incontinence diagnosed by history. It is yet to be established if this information benefits patients.
A prospective cross‐sectional study to define racial variation in fetal nasal bone length through ultrasound assessment at 18–20 weeks’ gestation
Authors: MOGRA Ritu , SCHLUTER Philip J. , OGLE Robert F. , O’CONNELL Justine , FORTUS Lou , HYETT Jon A. 
Published: September 2010

Objective:  An absent or short nasal bone is highly predictive of Down syndrome in Caucasian populations, but Asians may have shorter nasal bones – increasing the false positive rate of screening. We examine differences in nasal bone length (NBL) in Caucasian and Asian populations.Methods:  This prospective cohort study involved pregnant women attending for their routine anomaly scan at 18–20 weeks’ gestation. Ethnicity of the patient and their partner was recorded, and the nasal bone was measured three times. Mean NBL was calculated and used to investigate the effect of ethnicity first with a simple linear regression model and second with a mixed‐effects regression model that accounted for variability of measurement between sonographers.Results:  A total of 1087 families were involved in the study, including 592 (54%) Caucasians, 214 (20%) East Asians, 110 (10%) South Asians and 171 (16%) West Asians. Twenty‐three sonographers performed the scans with an average of 19 scans each. There is no significant difference in NBL between Caucasian and Asian populations. The mixed‐effects model shows that accounting for sonographer variation is important, with 6.7% of the total variance in measurement being related to this random effect.Conclusions:  There is no significant difference in NBL between Caucasian and Asian populations. It is reasonable to use criteria established in a Caucasian population to define the characteristics of an absent/short nasal bone in Asian fetuses. This finding also removes difficulties in counselling mixed race couples.
The Impact of Pretransplant Dialysis on Outcomes in Renal Transplantation
Authors: Meier‐Kriesche Herwig‐Ulf , Schold Jesse D. 
Published: September 2010

ABSTRACTDialysis is the most common therapeutic intervention for patients with end‐stage renal disease (ESRD). The demonstration of a clear survival benefit associated with renal transplantation has made it the preferred treatment option for ESRD patients medically cleared for transplant. This has invoked a shift in thinking regarding the timing of transplantation. Impaired renal function and particularly ESRD with dialysis are significant cardiovascular risk factors for this population. Part of these cumulative effects can probably be avoided by transplantation without prior dialysis. In fact, the evidence to date demonstrates a significant advantage for allograft and patient survival associated with preemptive transplantation. In addition, preemptive transplantation is associated with better quality of life for these patients and is less costly than dialysis. The key for patients approaching ESRD is early referral to a transplant center to explore the most appropriate treatment options in a timely fashion. In fact, it is better to transplant patients preemptively than to wait until they reach ESRD and start dialysis.
HEMATOLOGY: ISSUES IN THE DIALYSIS PATIENT: Anemia Management in Chronic Kidney Disease: What Have We Learned after 17 Years?
Authors: Spiegel David M. 
Published: September 2010

ABSTRACTAnemia management remains controversial in patients with chronic kidney disease (CKD). The overwhelming conclusion from retrospective data analyses show a strong correlation between higher hemoglobin values and improved outcomes, including mortality, hospitalization, left ventricular hypertrophy, and quality of life. However, the evidence available from two large prospective trials in CKD comparing hemoglobin values near the current target of 11–12 g/dl with normalization or near normalization were stopped, as there was a trend toward worsening outcomes in the higher hemoglobin groups. This disparity suggests that patients who have or achieve high hemoglobin values on low doses or no erythropoietin may be inherently different from the general CKD population. Therefore targeting to a hemoglobin value above the current recommendation with intermittent erythropoietin seems unwarranted until randomized prospective trials demonstrate both safety and efficacy.
HEMATOLOGY: ISSUES IN THE DIALYSIS PATIENT: Intravenous Iron Therapy in End‐Stage Renal Disease
Authors: Brewster Ursula C. 
Published: September 2010

ABSTRACTIntravenous iron therapy is instrumental in the management of anemia in patients with end‐stage renal disease (ESRD). Iron is available in several different preparations, with slight differences in the pharmacology of each. Given the importance of intravenous iron in the management of these patients, clinicians should be aware of the potential risks associated with it. Intravenous iron has effects on host immunity that raise concerns about clinical infection risk. Iron preparations appear to increase oxidative stress in these patients, which has important implications for cardiovascular disease states. Lastly, the effects of intravenous iron on liver disease are largely unknown.
HEMATOLOGY: ISSUES IN THE DIALYSIS PATIENT: Platelet Dysfunction and End‐Stage Renal Disease
Authors: Kaw Dinkar , Malhotra Deepak 
Published: September 2010

ABSTRACTPatients with end‐stage renal disease (ESRD) develop hemostatic disorders mainly in the form of bleeding diatheses. Hemorrhage can occur at cutaneous, mucosal, or serosal sites. Retroperitoneal or intracranial hemorrhages also occur. Platelet dysfunction is the main factor responsible for hemorrhagic tendencies in advanced kidney disease. Anemia, dialysis, the accumulation of medications due to poor clearance, and anticoagulation used during dialysis have some role in causing impaired hemostasis in ESRD patients. Platelet dysfunction occurs both as a result of intrinsic platelet abnormalities and impaired platelet–vessel wall interaction. The normal platelet response to vessel wall injury with platelet activation, recruitment, adhesion, and aggregation is defective in advanced renal failure. Dialysis may partially correct these defects, but cannot totally eliminate them. The hemodialysis process itself may in fact contribute to bleeding. Hemodialysis is also associated with thrombosis as a result of chronic platelet activation due to contact with artificial surfaces during dialysis. Desmopressin acetate and conjugated estrogen are treatment modalities that can be used for uremic bleeding. Achieving a hematocrit of 30% improves bleeding time in ESRD patients.
Small Bowel Volvulus in Dogs Clinical Observations
Authors: HARVEY H. JAY , RENDANO VICTOR T. 
Published: September 2010

Small bowel volvulus was diagnosed in six dogs. Peracute onset, hematochezia, and lack of vomition were the most prominent clinical features. All affected dogs exceeded 23 kg in body weight. Five of the six dogs were males and two were German shepherd dogs.There were no diagnostic findings that were specific for intestinal volvulus. Abdominal radiographs in two dogs were interpreted as showing “pathologic” dilatation of bowel. However, it could not be determined whether the dilatation was caused by adynamic ileus or by mechanical obstruction. Results of hematological tests help to differentiate other causes of bloody diarrhea such as hemorrhagic gastroenteritis and parvovirus enteritis.Prognosis for dogs with intestinal volvulus was poor: all six of the dogs in our series died.
Screening plateletpheresis donors for HLA antibodies on two high‐throughput platforms and correlation with recipient outcome
Authors: Quillen Karen , Medrano Consuelito , Adams Sharon , Peterson Brett , Hackett Julia , Leitman Susan F. , Klein Harvey G. , Stroncek David F. 
Published: September 2010

BACKGROUND: To determine the prevalence and impact of transfusing plasma containing white blood cell antibodies, we compared two high‐throughput HLA antibody screening assays and prospectively examined the medical records of all platelet (PLT) recipients to detect subtle manifestations of transfusion‐related acute lung injury and other transfusion reactions.STUDY DESIGN AND METHODS: Serum samples from 136 plateletpheresis donors were tested for HLA Class I and II antibodies using microbead (LABScreen PRA, One Lambda) and microchip (Dynachip, Invitrogen) assays. Electronic medical records of all recipients were reviewed for vital signs and nursing documentation before and after transfusion.RESULTS: In the microchip assay with a cutoff value of 0.25, 2.9% of samples were positive for Class I and 8.9% for Class II antibodies; with a cutoff value of 0.1, the results were 14.9 and 21.6%, respectively. In the microbead assay (normalized background ratio, 1.5), 15% were positive for Class I and 21% for Class II antibodies. The prevalence of HLA antibodies was 17% in donors without pregnancy or transfusion history and 47% in donors with such history. The PLTs were transfused in 265 episodes to 67 patients. There were no reported reactions; however, symptoms or vital sign changes were noted in seven transfusion episodes. The incidence of reactions was 2.7% (2/75) for antibody‐positive units and 2.6% (5/190) for antibody‐negative units.CONCLUSIONS: Microbead and microchip assays yielded similar results. The prevalence of HLA antibodies was greater in donors with a history of pregnancy or transfusion, but no increase in the incidence of transfusion reactions was noted in recipients of components from donors with HLA antibodies.
The impact of temporary deferral due to low hemoglobin: future return, time to return, and frequency of subsequent donation
Authors: Hillgrove Tessa , Moore Vivienne , Doherty Kathleen , Ryan Philip 
Published: September 2010

BACKGROUND: This study investigated the effects of a 6‐month deferral due to low hemoglobin (Hb) on the subsequent donation patterns of Australian whole blood donors.STUDY DESIGN AND METHODS: The study was a retrospective cohort study of the donation patterns of all whole blood donors deferred for low Hb during a 2‐month period compared with donors who were not deferred. Donations 3 years after eligibility to give blood were recorded. Analysis of proportion returning, time to return, and frequency of donation was performed using logistic regression, survival analysis, and negative binomial regression.RESULTS: Among first‐time donors, 20.9% of low Hb–deferred donors returned during the follow‐up period versus 69.9% of those not deferred. Among repeat donors, 64.0% of deferred donors returned versus 91.0% of those not deferred. Temporary deferral delayed time to first return (p < 0.001), reduced frequency of donation (2.4 donations per donor before deferral compared to 1.1 per donor in first year of follow‐up), and increased the likelihood of dropping out in later years of follow‐up. However, if a donor returned promptly once eligible and gave more donations in the first year, the negative impact on future donation patterns was diminished. High frequency of attendance before deferral was the strongest predictor of time to return and future donation frequency.CONCLUSION: Deferral for low Hb had a strong effect on first‐time and repeat donors. This study highlights the influence of strong donation habits on return after deferral and the importance of encouraging donors to return promptly once eligible.
Functional importance of a conserved sequence motif in FhaC, a prototypic member of the TpsB/Omp85 superfamily
Authors: Delattre Anne‐Sophie , Clantin Bernard , Saint Nathalie , Locht Camille , Villeret Vincent , Jacob‐Dubuisson Françoise 
Published: September 2010

In Gram‐negative bacteria, the two‐partner secretion pathway mediates the secretion of TpsA proteins with various functions. TpsB transporters specifically recognize their TpsA partners in the periplasm and mediate their transport through a hydrophilic channel. The filamentous haemagglutinin adhesin (FHA)/FhaC pair represents a model two‐partner secretion system, with the structure of the TpsB transporter FhaC providing the bases to decipher the mechanism of action of these proteins. FhaC is composed of a β‐barrel preceded by two periplasmic polypeptide‐transport‐associated (POTRA) domains in tandem. The barrel is occluded by an N‐terminal helix and an extracellular loop, L6, folded back into the FhaC channel. In this article, we describe a functionally important motif of FhaC. The VRGY tetrad is highly conserved in the TpsB family and, in FhaC, it is located at the tip of L6 reaching the periplasm. Replacement by Ala of the invariant Arg dramatically affects the secretion efficiency, although the structure of FhaC and its channel properties remain unaffected. This substitution affects the secretion mechanism at a step beyond the initial TpsA–TpsB interaction. Replacement of the conserved Tyr affects the channel properties, but not the secretion activity, suggesting that this residue stabilizes the loop in the resting conformation of FhaC. Thus, the conserved motif at the tip of L6 represents an important piece of two‐partner secretion machinery. This motif is conserved in a predicted loop between two β‐barrel strands in more distant relatives of FhaC involved in protein transport across or assembly into the outer membranes of bacteria and organelles, suggesting a conserved function in the molecular mechanism of transport.Structured digital abstract
MINT‐7996294: Fha30 (uniprotkb:P12255) binds (MI:0407) to FhaC (uniprotkb:P35077) by filter binding (MI:0049)