bluuebunny/crossref_metadata_2025_split huggingface dataset:
The influence of physical rehabilitation of patients on the course of chronic heart failure
Authors: Tokmachev R. E. , Kravchenko A. Ya. , Budnevsky A. V. , Kontsevaya А. V. , Simion A. Yu. , Chernyshova N. I. , Chumachenko A. V. 
Published: May 2024

IIntriduction. Complex rehabilitation programs makes an important role in improving exercise tolerance and patients functional status with cardiovascular pathology.Aim. To study the physical rehabilitation effect on the functional status, pro-inflammatory cytokines levels and cardiovascular inflammation biomarker (high-sensitivity C-reactive protein) in heart failure patients with different ejection fractions.Materials and methods. The study included 160 patients with chronic heart failure (84 men and 76 women, mean age 69.4 ± 8.8 years). Two groups of patients with preserved and reduced left ventricular ejection fraction were identified. The groups were divided into two subgroups: the first – those who underwent physical rehabilitation for a year and received standard drug therapy; the second one received exclusively standard drug therapy. At inclusion and after a year of observation, patients were examined: determination of functional status, levels of pro-inflammatory cytokines, serum hs-C-reactive protein, NT-proBNP.Results. Regardless of left ventricular ejection fraction, in the studied patients subgroups undergone physical rehabilitation, a statistically significant decrease in the proinflammatory cytokines levels, NT-proBNP and hs-C-reactive protein was noted. The study also demonstrated the physical rehabilitation beneficial effects on the patients with HF functional status. Thus, the distance walked within 6 minutes and its relation to the proper indicator in patients who underwent physical rehabilitation increased in comparison with the initial indicators during the initial examination of patients and in comparison with control subgroups. This may indicate the positive properly selected physical activity impact on circulatory compensation and the HF course.Conclusion. Carrying out physical rehabilitation for chronic heart failure leads to an improvement in functional status, a decrease in the NT-proBNP, pro-inflammatory cytokines and high-sensitivity C-reactive protein level in comparison with the results of managing patients on drug therapy (without rehabilitation).
When it comes to lead in water, new biosensing technology can reveal what the eyes cannot see and what the rules do not yet stop
Authors: Weinstock Robert , Young Sera L. , Knaus Alyssa , Messing Jenna , Bly Vanessa , Lucks Julius B. 
Published: May 2024

ABSTRACT
Deficiencies in knowledge about water quality prevent or obscure progress on a panoply of public health problems globally. Specifically, such lack of information frustrates effective and efficient government regulation to protect the public from contaminated drinking water. In this Practical Paper, we lay out how recent scientific innovations in synthetic biology mean that rapid, at-home tests based on biosensor technology could be used to improve water quality monitoring and regulation, using the example of the U.S. Environmental Protection Agency's Lead and Copper Rule currently under revision. Biosensor tests can be used by non-scientists and the information that biosensor tests generate is relatively cheaper and faster than standard laboratory techniques. As such, they have the potential to make it possible to increase the number and frequency of samples tested. This, in turn, could facilitate more accurate compliance monitoring, justify more protective substantive standards, and more efficiently identify infrastructure priorities. Biosensors can also empower historically underrepresented communities by facilitating the visibility of inequities in lead exposure, help utilities to ensure safe water delivery, and guide policy for identifying and replacing lead-bearing water infrastructure, thereby improving public health. As the technology matures, biosensors have great potential to reveal water quality issues, thereby reducing public health burdens.
Revolutionizing Infertility Management through Novel Peptide-based
Targets
Authors: Kumar Vijay , Doshi Gaurav 
Published: May 2024

:
Around 48 million couples and 186 million people worldwide have infertility; of these,
approximately 85% have an identifiable cause, the most common being ovulatory dysfunctions,
male infertility, polycystic ovary syndrome, and tubule disease. The remaining 15% have infertility
for unknown reasons, including lifestyle and environmental factors. The regulation of the hypothalamic-
pituitary-adrenal axis (HPA) is crucial for the secretion of gonadotropin-releasing hormone
(GnRH), luteinizing hormone (LH), and follicle-stimulating hormone (FSH), which are essential
for female reproductive functions. GnRH is the primary reproductive axis regulator. The
pattern of GnRH, FSH, and LH release is determined by its pulsatile secretion, which in turn controls
endocrine function and gamete maturation in the gonads. Peptides called Kisspeptin (KP),
Neurokinin-B (NKB), and Orexin influence both positive and negative feedback modulation of
GnRH, FSH, and LH secretion in reproduction. This review article mainly focuses on the historical
perspective, isoform, and signaling pathways of KP, NKB, and Orexin novel peptide-based targets
including clinical and preclinical studies and having a promising effect in the management of
infertility.
Overexpression of MiR-188-5p Downregulates IL6ST/STAT3/ NLRP3 Pathway to Ameliorate Neuron Injury in Oxygen-glucose Deprivation/Reoxygenation
Authors: Hu Yujie , Wang Ganlan , Yang Guoshuai 
Published: May 2024

Background:
CI/R, characterized by ischemic injury following abrupt reestablishment
of blood flow, can cause oxidative stress, mitochondrial dysfunction, and apoptosis.
We used oxygen-glucose deprivation/reoxygenation (OGD/R) induced injury in HT22
and primary mouse cortical neurons (MCN) as a model for CI/R.

Objective:
This study investigates the role of miR-188-5p in hippocampal neuron cell injury
associated with Cerebral Ischemia-Reperfusion (CI/R).

Methods:
HT22 and MCN cells were induced by OGD/R to construct an in vitro model of
CI/R. Cell apoptosis and proliferation were assessed using flow cytometry and the Cell
Counting Kit-8 (CCK8). ELISA was conducted to measure the levels of IL-1β, IL-6, and
TNF-α. Moreover, the interaction between miR-188-5p and IL6ST was investigated using
dual luciferase assay, the expression of miR-188-5p, Bax, cleaved-caspase3, IL-6, Bcl-2,
IL-1β, TNF-α, IL6ST, NFκB, NLRP3 and STAT3 was evaluated using RT-qPCR or Western
blot, and immunofluorescence was used to analyze the co-expression of p-STAT3 and
NLRP3 in neuronal cells.

Results:
OGD/R reduced proliferation and miR-188-5p levels and increased IL6ST expression,
inflammation, and apoptosis in HT22 and MCN cells. Moreover, miR-188-5p was
found to bind to IL6ST. Mimics of miR-188-5p reduced apoptosis, lowered the expression
of cleaved-caspase3 and Bax proteins, and elevated Bcl-2 protein expression in cells treated
with OGD/R. Overexpression of miR-188-5p decreased the levels of NLRP3 and p-STAT3
in the OGD/R group. Furthermore, the overexpression of miR-188-5p reduced IL6ST, p-
NFκB/NFκB, p-STAT3/STAT3, and NLRP3 proteins in OGD/R, and these effects could be
reversed by IL6ST overexpression.

Conclusion:
Mimics of miR-188-5p were found to inhibit inflammation and the
STAT3/NLRP3 pathway via IL6ST, thereby ameliorating injury in HT22 and MCN cells
treated with OGD/R in the context of CI/R.
Hydroxyapatite as Nanoparticle-based Adjuvant: Leishmaniasis Vaccine Candidate
Authors: Kelleci Kübra 
Published: May 2024

Introduction:
Leishmaniasis caused by Leishmania protozoa can be fatal if left untreated.
An effective and safe human vaccine has still not been produced to eradicate the disease. Among
vaccine development studies, dead vaccines are still known as the most reliable and cheapest method.
The selection of an effective and safe adjuvant is important for killed vaccines. We have not
found any studies in which hydroxyapatite particles were used as adjuvants in vaccine research
against leishmaniasis.

Method:
In this study, spherical Hydroxyapatite (HAp) nanoparticles with dimensions of 100nm
were synthesized. Then, these particles were combined with autoclaved L. infantum antigens (ALA)
to prepare vaccine formulations at different concentrations. To determine the immunogenicity of
HAp, MTT cell viability analysis, nitric oxide (NO), and cytokine production abilities were investigated
in vitro in J774 macrophage cells.

Result:
According to the study results, it was determined that the cell viability level was 97 % at a
concentration of 200 μg/ml, and there was a 10-fold increase in NO production and an approximately
8.5-fold increase in IL-12 cytokine production ability compared to the control group.

Conclusion:
Considering the study results and the non-toxic properties of HAp, we have shown that
HAp can be used as an adjuvant in the development of new leishmania vaccines.
The Different Body Mass Index Does Not Impact Physical Health in Older Adults without Sedentary Behavior: A Cross-Sectional Study
Authors: Amput Patchareeya , Wongphon Sirima 
Published: May 2024

Aims and Objective:
This study aimed to compare and determine the correlation of
BMI on physical health, including muscle strength, balance, and cardiorespiratory fitness in older
adults without sedentary behavior.

Methods:
A total of 65 older adults without sedentary behavior were recruited and divided into 3
groups on the basis of body mass index (BMI): normal weight (n= 22/group), overweight (n= 22/-
group), and obese (n= 21/group). All the older adults were evaluated for upper limb muscle
strength using the handgrip strength test, lower limb muscle strength using the sit-to-stand (STS10)
test, balance ability using the time up and go (TUG) test, and cardiorespiratory fitness using
the 6-minute walk test (6MWT).

Results:
The handgrip strength test, STS10 test, and distance of 6MWT showed no difference
among the groups (p> 0.05). However, overweight group had a significantly longer duration for
the TUG test when compared to normal weight, while obese group had a significantly longer duration
for the TUG test when compared with overweight and normal weight groups (p< 0.01). In addition,
BMI was positively correlated with the duration of the TUG test. Whereas, the handgrip
strength test, STS10 test, and distance of 6MWT were not correlated with BMI.

Conclusion:
The BMI does not impact upper and lower limb muscle strength or the cardiorespiratory
fitness test, except for the ability to balance in older adults without sedentary behavior.
A Mutation in the CACNA1F Gene Found by Whole Exome Sequencing (WES) and In Silico Analysis in an Iranian Family with Consanguineous Relationships
Authors: Omarmeli Vahid , Assefi Marjan , Lewandrowski Kai-Uwe , Sharafshah Alireza , Faizmahdavi Hanieh , Darabi Parichehr , Amiri Amir , Mansouri Nasrin 
Published: May 2024

Background:
X-linked mutations are highly important in clinical diagnosis, and at least
533 disorders are related to the genes located on the X chromosome.

Case Presentation:
A 21-year-old Caucasian woman with a 24-year-old Caucasian man as her
fiancé referred Clinical genetic lab for premarital genetic counseling (carrier screening). None of
them had any abnormal manifestations. Following genetic counseling, Whole Exome Sequencing
(WES) test performed to find the possible pathogenic mutations. Also, after drawing the couple's
pedigree, candidate mutations were examined in the woman's parents as well as her uncles. Additionally,
in silico investigations were performed through SWISS-MODEL, MolProbity, ProSA, Py-
Mol, and FATCAT tools. The most important mutation diagnosed in the woman (R1362Q in the
35th exon of CACNA1F), was observed in her mother and her two uncles. The mutation was also
screened in both her father and her fiancé, but they had no mutations. After medical examinations
of carriers, there was no sign of any eye impairment. Other mutations were TCTN2
(c.1613-2A>G), TARS (p.K319E), SPEG (p.E3020K), CPS1 (p.A1180V), MYO3A (p.I736M),
NNT (p.R968Q), MED23 (p.K406T). Bioinformatics analyses indicated no alteration in the mutant
structure of CACNA1F (Q1362) compared with the normal structure (R1362).

Conclusion:
Conclusively, the current study emphasizes the non-pathogenic effect of missense
mutation R1362Q in the 35th exon of CACNA1F in association with ocular diseases. This will ensure
the reports of this mutation as healthy instead of uncertain in the literature and databanks.
Culinary Studios as an Innovative Product for the Popularization of Domestic Tourism in the Kursk Region
Authors: Abushenkova M. V. , Smirnova M. A. 
Published: May 2024

The relevance of the study is determined by the need to assess the introduction of culinary studios into the restaurant business as an innovative product of the development of the gastronomic space of the Kursk region. The purpose of the article is to provide an economic justification for the introduction of culinary studios into the gastronomic space of the Kursk region in order to popularize domestic tourism in the region. Objectives: to study the theoretical foundations of gastronomic tourism; to consider the historical aspect of the research topic; to evaluate the experience of using culinary and gastronomic studios in the Kursk region; to develop a strategy for reforming the tourist product and the optimal ways to integrate it into the infrastructure of the restaurant business in the region.Methodology. To study scientific articles, works of domestic specialists, statistical data and other sources related to the research topic, the authors of the article relied on general scientific methods such as analysis, observation, description, comparison, graphical representation, synthesis.Results. Based on the study of the development of the gastronomic space of the Kursk region, recommendations were formed on the introduction of culinary studios into the restaurant business to increase the tourist flow and popularize domestic tourism in the region.Conclusions. During the study, the authors conclude that the creation of culinary studios and their introduction into the restaurant business of the Kursk region will contribute to the comprehensive development of gastronomic culture and tourism in the region. With the help of this tool, the personal brand of the Nightingale Region will be able to attract attention, increase interest in its culture and history, as well as stimulate the development of small and medium-sized businesses in the catering and tourism industry, including by attracting investors.
An Innovative Approach to the Organization of the Company's Activities Based on the Use of Digital Technologies for Brand Promotion
Authors: Ponomarev S. V. , Krainova O. S. , Moskovtseva L. V. , Plyasova S. V. 
Published: May 2024

The relevance of the research is determined by the need to adapt the activities of enterprises to the rapidly developing digital environment, which requires regular revision of the promotion strategy and adaptation to the new changing conditions shaping the business landscape. The purpose of the study is to develop an innovative approach to the organization of the company's activities based on the use of digital technologies for brand promotion in the face of changes, as well as to determine the directions of their practical implementation.The objectives of the research are to determine the theoretical and methodological foundations for the formation and implementation of a brand promotion strategy in the company's activities; substantiate the role of digital technologies in the brand promotion strategy of a modern enterprise; develop an innovative approach to the organization of the company's activities based on the use of digital brand promotion technologies.Methodology. The paper uses the method of theoretical analysis, the method of classification, synthesis, deduction and induction. The following practical research methods are used: observation method, analysis of collected data, description, economic analysis, comparison method, systematic approach. The applied empirical research methods include: choosing a platform for posting content, ways and channels of influencing the consumer audience of an Internet resource. The results. The elements of an innovative approach to the organization of an enterprise's activities based on the use of digital brand promotion technologies are revealed: the procedure for analyzing content and determining its compliance with the target audience; segments of the target audience for interaction in the process of brand promotion; main and additional promotion channels; options for creating a communication strategy; metrics used to assess the effectiveness of a brand promotion strategy. The stages of digital content creation are highlighted and the author's recommendations for their improvement are proposed.Conclusions. An innovative approach to brand promotion using digital promotion technologies is presented, the uniqueness of which consists in the use of updated basic elements and digital tools at each stage of the strategy, allowing to increase the adaptability of the enterprise to changing environmental conditions.
Digital Scientific and Educational Environment of the University: from Theory to Practice
Authors: Abramov A. P. , Afanas'eva V. A. , Suprun A. Yu. , Smirnova M. A. 
Published: May 2024

Relevance.The use of digital tools has become a reality in all spheres of human activity in the last decade. The modern university has become a testing ground for technological innovations, in which digitalization is not only a managerial and scientific driver, but also a constant practice of educational relations.At the same time, the process of implementation and use of the digital scientific and educational environment is due to a number of circumstances (problems) that need to be overcome specifically in scientific interdisciplinary discourse: firstly, the ongoing discussions around the dyad “human - artificial intelligence” and the uncertainty of the boundaries of the co-evolving unity of its components; secondly, the existing request of the university community for their implementation and the infrastructural limitations of using digital tools in the educational environment of a particular educational institution.Therefore, the digital scientific and educational environment of the university remains the focus of close attention of researchers and the entire scientific community.The purposeis determine directions for improving the digital scientific and educational environment of a modern Russian university Objectives:characterize theoretical and methodological approaches to the creation and use of a digital scientific and educational environment; identify problems and prospects for implementing management practices for its implementation; to specify effective ways of innovative changes.Methodology.The methodological discourse is built in line with the main problem of our time ‒ the contradiction between the achievements of scientific and technological progress on the one hand and spiritual and moral culture on the other. The conceptual “portfolio” of the study is formed from the main provisions of social theories of educational management using quantitative and qualitative methods of data collection and analysis.Results.A theoretical "portfolio" of concepts and approaches has been defined that explain the sociocultural nature of the digital scientific and educational environment of the university. Based on empirical data based on surveys and expert assessments, the pros and cons of introducing digital technologies are identified and directions for their improvement are outlinedConclusions.Digitalization is an element of inevitable progress and transformation of all spheres of human activity. The digital scientific and educational environment of the university is intended to become a trigger for innovative changes in educational relations.
Prospects for the Development of the Russian Ferrous Metallurgy in the Context of Sanctions
Authors: Shmanev S. V. 
Published: May 2024

Relevance. Currently, ferrous metallurgy plays an important role in the Russian economy. The ferrous metallurgy industry accounts for approximately 5-6% of GDP and is a key indicator of the country's technological development and production capacity. It affects various spheres of society, including construction, mechanical engineering, and the automotive industry. Ferrous metallurgy is also relevant because of its connection with environmental and energy problems. However, for the successful development of this industry, systematic work is needed to modernize technologies, ensure energy efficiency, and comply with environmental standards.The purpose is to analyze the effectiveness of the functioning of the domestic ferrous metallurgy as the basis for sustainable socio-economic development of the country in modern conditions and identify trends in the development of industry in the context of sanctions and global economic stagnation.Objectives: it is necessary to identify whether the policy of import substitution established by Russia and the adopted Strategy for the Development of the metallurgical industry of the Russian Federation for the period up to 2030 contributes to economic growth, improving national security and ensuring a decent standard of living for the population of the country.Methodology. The above study used the works of Russian scientists and the analysis of materials by Russian experts. Methods of comparison, analysis, synthesis and forecasting were used in the study.Results. Ferrous metallurgy, based on advanced technologies, is not only an indicator of economic and social development but also forms the basis of the country's economic independence and security.Conclusions. The development of ferrous metallurgy without the revival of domestic engineering and the replacement of imported machines and technologies with their domestic products will lead to a further increase in dependence on the Western economy and the goals of Western politicians, and this is not only a matter of security, but also the existence of the state itself.