bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of nuclear factor-kappa B differentially affects thyroid cancer cell growth, apoptosis, and invasion
Authors: Bauerle Kevin T , Schweppe Rebecca E , Haugen Bryan R 
Published: May 2010

Abstract

Background
Nuclear factor-κB (NF-κB) is constitutively activated in many cancers and plays a key role in promoting cell proliferation, survival, and invasion. Our understanding of NF-κB signaling in thyroid cancer, however, is limited. In this study, we have investigated the role of NF-κB signaling in thyroid cancer cell proliferation, invasion, and apoptosis using selective genetic inhibition of NF-κB in advanced thyroid cancer cell lines.

Results
Three pharmacologic inhibitors of NF-κB differentially inhibited growth in a panel of advanced thyroid cancer cell lines, suggesting that these NF-κB inhibitors may have off-target effects. We therefore used a selective genetic approach to inhibit NF-κB signaling by overexpression of a dominant-negative IκBα (mIκBα). These studies revealed decreased cell growth in only one of five thyroid cancer cell lines (8505C), which occurred through a block in the S-G2/M transition. Resistance to TNFα-induced apoptosis was observed in all cell lines, likely through an NF-κB-dependent mechanism. Inhibition of NF-κB by mIκBα sensitized a subset of cell lines to TNFα-induced apoptosis. Sensitive cell lines displayed sustained activation of the stress-activated protein kinase/c-Jun NH2-terminal kinase (SAPK/JNK) pathway, defining a potential mechanism of response. Finally, NF-κB inhibition by mIκBα expression differentially reduced thyroid cancer cell invasion in these thyroid cancer cell lines. Sensitive cell lines demonstrated approximately a two-fold decrease in invasion, which was associated with differential expression of MMP-13. MMP-9 was reduced by mIκBα expression in all cell lines tested.

Conclusions
These data indicate that selective inhibition of NF-κB represents an attractive therapeutic target for the treatment of advanced thyroid. However, it is apparent that global regulation of thyroid cancer cell growth and invasion is not achieved by NF-κB signaling alone. Instead, our findings suggest that other important molecular processes play a critical role in defining the extent of NF-κB function within cancer cells.
Study of stability of time-domain features for electromyographic pattern recognition
Authors: Tkach Dennis , Huang He , Kuiken Todd A 
Published: May 2010

Abstract

Background
Significant progress has been made towards the clinical application of human-machine interfaces (HMIs) based on electromyographic (EMG) pattern recognition for various rehabilitation purposes. Making this technology practical and available to patients with motor deficits requires overcoming real-world challenges, such as physical and physiological changes, that result in variations in EMG signals and systems that are unreliable for long-term use. In this study, we aimed to address these challenges by (1) investigating the stability of time-domain EMG features during changes in the EMG signals and (2) identifying the feature sets that would provide the most robust EMG pattern recognition.

Methods
Variations in EMG signals were introduced during physical experiments. We identified three disturbances that commonly affect EMG signals: EMG electrode location shift, variation in muscle contraction effort, and muscle fatigue. The impact of these disturbances on individual features and combined feature sets was quantified by changes in classification performance. The robustness of feature sets was evaluated by a stability index developed in this study.

Results
Muscle fatigue had the smallest effect on the studied EMG features, while electrode location shift and varying effort level significantly reduced the classification accuracy for most of the features. Under these disturbances, the most stable EMG feature set with combination of four features produced at least 16.0% higher classification accuracy than the least stable set. EMG autoregression coefficients and cepstrum coefficients showed the most robust classification performance of all studied time-domain features.

Conclusions
Selecting appropriate EMG feature combinations can overcome the impact of the studied disturbances on EMG pattern classification to a certain extent; however, this simple solution is still inadequate. Stabilizing electrode contact locations and developing effective classifier training strategies are suggested to further improve the robustness of HMIs based on EMG pattern recognition.
Coexistence of mal de Meleda and congenital cataract in a consanguineous Tunisian family: two case reports
Authors: Bchetnia Mbarka , Merdassi Ahlem , Charfeddine Cherine , Mgaieth Fatma , Kassar Selma , Ouechtati Farah , Chouchene Ibtissem , Boussen Hamouda , Mokni Mourad , Osman Amel Dhahri-Ben , Boubaker Med Samir , Abdelhak Sonia , Elmatri Leila 
Published: May 2010

Abstract

Introduction
Mal de Meleda is a rare form of palmoplantar keratoderma, with autosomal recessive transmission. It is characterized by diffuse erythema and hyperkeratosis of the palms and soles. Recently, mutations in the ARS (component B) gene (ARS, MIM: 606119) on chromosome 8q24.3 have been identified in families with this disorder. Congenital cataract is a visual disease that may interfere with sharp imaging of the retina. Mutations in the heat-shock transcription factor 4 gene (HSF4; MIM: 602438) may result in both autosomal dominant and autosomal recessive congenital cataracts.

Case presentation
A Tunisian family with two female siblings aged 45 and 30 years, presented with a clinical association of mal de Meleda and congenital cataract. The two patients exhibited diffuse palmoplantar keratodermas. One of them presented with a total posterior subcapsular cataract and had a best corrected visual acuity at 1/20 in the left eye and with the right eye was only able to count fingers at a distance of one foot. The other woman had a slight posterior subcapsular lenticular opacity and her best corrected visual acuity was 8/10 in the right eye and with her left eye she was only able to count fingers at a distance of one foot. A mutational analysis of their ARS gene revealed the presence of the homozygous missense mutation C99Y and two single nucleotide polymorphisms (-55G>C and -60G>C). The splice mutation (c.1327+4A-G) within intron 12 of the HSF4 gene, which has been previously described in Tunisian families with congenital cataract, was not found in the two probands within this family.

Conclusion
To the best of our knowledge, such original clinical association has not been reported previously. The association of these two autosomal recessive diseases might have occurred in this family due to a high degree of inbreeding. The C99Y mutation may be specific to the Tunisian population as it has been exclusively reported so far in only three Tunisian families with mal de Meleda.
Extracorporeal life support for management of refractory cardiac or respiratory failure: initial experience in a tertiary centre
Authors: Peris Adriano , Cianchi Giovanni , Biondi Simona , Bonizzoli Manuela , Pasquini Andrea , Bonacchi Massimo , Ciapetti Marco , Zagli Giovanni , Bacci Simona , Lazzeri Chiara , Bernardo Pasquale , Mascitelli Erminia , Sani Guido , Gensini Gian Franco 
Published: May 2010

Abstract

Introduction
Extracorporeal Life Support (ECLS) and extracorporeal membrane oxygenation (ECMO) have been indicated as treatment for acute respiratory and/or cardiac failure. Here we describe our first year experience of in-hospital ECLS activity, the operative algorithm and the protocol for centralization of adult patients from district hospitals.

Methods
At a tertiary referral trauma center (Careggi Teaching Hospital, Florence, Italy), an ECLS program was developed from 2008 by the Emergency Department and Heart and Vessel Department ICUs. The ECLS team consists of an intensivist, a cardiac surgeon, a cardiologist and a perfusionist, all trained in ECLS technique. ECMO support was applied in case of severe acute respiratory distress syndrome (ARDS) not responsive to conventional treatments. The use of veno-arterial (V-A) ECLS for cardiac support was reserved for cases of cardiac shock refractory to standard treatment and cardiac arrests not responding to conventional resuscitation.

Results
A total of 21 patients were treated with ECLS during the first year of activity. Among them, 13 received ECMO for ARDS (5 H1N1-virus related), with a 62% survival. In one case of post-traumatic ARDS, V-A ECLS support permitted multiple organ donation after cerebral death was confirmed. Patients treated with V-A ECLS due to cardiogenic shock (N = 4) had a survival rate of 50%. No patients on V-A ECLS support after cardiac arrest survived (N = 4).

Conclusions
In our centre, an ECLS Service was instituted over a relatively limited period of time. A strict collaboration between different specialists can be regarded as a key feature to efficiently implement the process.
A prospective study of hearing preservation in untreated vestibular schwannomas
Authors: Sughrue Michael E. , Kane Ari J. , Kaur Rajwant , Barry Jeffrey J. , Rutkowski Martin J. , Pitts Lawrence H. , Cheung Steven W. , Parsa Andrew T. 
Published: May 2010

Object
The authors previously published a systematic review of the English language literature regarding the natural history of untreated vestibular schwannomas (VSs). This analysis found that the best predictor of future hearing loss was tumor growth > 2.5 mm/year on serial imaging, a factor that doubled the rate of hearing loss. In this paper the authors present an analysis of prospectively collected outcomes in patients with untreated VS from their institution that confirms their previous findings.

Methods
Clinical, radiographic, and audiometric data for all patients evaluated for VS at the authors' institution over a 22-year period were prospectively collected in a database. All patients in this database who had serviceable hearing (American Academy of Otolaryngology-Head and Neck Surgery Grade A or B) on initial presentation were selected, and underwent serial observation. Magnetic resonance imaging and audiometric data were analyzed, and the time from presentation until hearing loss was analyzed using Kaplan-Meier analysis.

Results
Fifty-nine patients with VS who initially presented with serviceable hearing were treated conservatively over this period. Consistent with the authors' previous findings, patients with a tumor growth rate > 2.5 mm/year at any point during follow-up lost their hearing at a much faster rate than those who had slower growing tumors. The median time to hearing loss was 7.0 years in those patients with tumor growth rate > 2.5 mm/year compared to 14.8 years in the other patients (p < 0.0001). The estimated median time to hearing loss in the 3 initial tumor size groups was 11.6 years in the intracanalicular group, 10.3 years in the group with 0.1–1 cm extension into the CPA cistern, and 9.3 years in the group with > 1 cm extension into the CPA cistern (p value nonsignificant). Initial tumor size, age at diagnosis, and neurofibromatosis Type 2 status did not affect the time to loss of serviceable hearing. Interestingly, many patients who were followed up for more than a decade eventually lost their hearing, regardless of whether the tumor displayed any documented interval growth.

Conclusion
The authors confirmed the findings of their systematic review of the literature using a prospectively followed group of patients with untreated VS. Collectively, these data suggest that the expectation for more rapid hearing loss should be communicated to patients, and the decision for surgical or other intervention should be made in the context of the known risk of continued observation of fast growing tumors.
Treatment of traumatic brain injury with thymosin β4 in rats
Authors: Xiong Ye , Mahmood Asim , Meng Yuling , Zhang Yanlu , Zhang Zheng Gang , Morris Daniel C. , Chopp Michael 
Published: May 2010

Object
This study was designed to investigate the efficacy of delayed thymosin β4 (Tβ4) treatment of traumatic brain injury (TBI) in rats.

Methods
Young adult male Wistar rats were divided into the following groups: 1) sham group (6 rats); 2) TBI + saline group (9 rats); 3) and TBI + Tβ4 group (10 rats). Traumatic brain injury was induced by controlled cortical impact over the left parietal cortex. Thymosin β4 (6 mg/kg) or saline was administered intraperitoneally starting at Day 1 and then every 3 days for an additional 4 doses. Neurological function was assessed using a modified neurological severity score (mNSS), foot fault, and Morris water maze tests. Animals were killed 35 days after injury, and brain sections were stained for immunohistochemistry to assess angiogenesis, neurogenesis, and oligodendrogenesis after Tβ4 treatment.

Results
Compared with the saline treatment, delayed Tβ4 treatment did not affect lesion volume but significantly reduced hippocampal cell loss, enhanced angiogenesis and neurogenesis in the injured cortex and hippocampus, increased oligodendrogenesis in the CA3 region, and significantly improved sensorimotor functional recovery and spatial learning.

Conclusions
These data for the first time demonstrate that delayed administration of Tβ4 significantly improves histological and functional outcomes in rats with TBI, indicating that Tβ4 has considerable therapeutic potential for patients with TBI.
Transformations des productions lithiques du premier au second Mésolithique en Bretagne et en Irlande
Authors: Costa Laurent Jacques , Marchand Grégor 
Published: May 2010

The aim of this paper is to evaluate the available factors of the transformation of lithic production during the Mesolithic in north-west Europe on the basis of examples from Brittany and Ireland, at the turn of the 7th millennium cal. BC. Changes in lithic productions in these two areas -concerning all stages of the "chaîne opératoire”, i. e. techniques, methods and end products - are so important that it is possible to speak about a before and an after 7th millennium, or an Early and a Late Mesolithic. Many reasons can be advanced for these changes as a technical or economical shift due to environmental changes or a transformation of exploitation strategies of the available resources. However our study shows that the transformation of lithic production must be regarded as a shift in social organization of these communities, even if the causes of the social modification
are still unknown.
La technique du tournage utilisée à l'Âge du Bronze final pour la fabrication d'épingles de bronze trouvées en Bourgogne
Authors: Armbruster Barbara , Pernot Michel 
Published: May 2010

A technological study of the two-piece pins from the Villethierry hoard (Yonne, France) has been performed with the help of different approaches. Firstly examinations with a binocular magnifying lens were carried out. Metallographic sections were prepared with samples taken from three pins; they were examined by optical microscopy and analysed with a device associated with a scanning electron microscope. Results from ethnoarchaeology and experimental archaeology were used. Precise answers to technical questions about the shaping process allow us to propose a complete and detailed scheme for the fabrication of this type of pin. The use of several techniques may be underlined. The pin head was formed by secondary casting. A spinning device was used, particularly for the shaping of the wax model of the head. As a last remark, annealing treatment
occurred before the finishing steps of the decoration.
Premières tentatives d'interprétation spatiale d'un site rural du Bronze final à Changis-sur-Marne (Seine-et-Marne)
Authors: Lafage Françoise , Auxiette Ginette , Brunet Paul , Martial Emmanuelle , Matterne Véronique , Praud Ivan , Laplantine Nicolas 
Published: May 2010

kilometres to the east of Meaux. The plots already excavated cover about
40 hectares.
The occupation of the valley here includes a large protohistoric funerary
zone on the slope of the eroded former terrace. This overlooks about 500 m
of an alluvial layer descending gently to the Marne. The first 300 metres beyond the flood plain present a dwelling which developed at the end of the Early Neolithic, some twenty isolated burials, and protohistoric open agricultural
establishments. The latter follow one another on the same spot for
more than a millennium from the end of Late Bronze IIb/ IIIa (RSFO) until the late La Tène period with no important interruptions but with varying degrees of density. For the Late Bronze Age, two focal points have been discovered. One is merely probable and the almost total lack of artefacts has not helped its study; the second, much better documented, has given rise
to a search for markers of the slow evolution of this rural world.
Our working hypothesis is founded on the spatial approach of peasant households during the last two phases of the Late Bronze Age, at the time of the transition to the first Iron Age and Early/ Middle Hallstatt in the same location. It is based on the cross-referencing of all data regarding the vestiges studied and gathered during the successive excavation campaigns: the classification of the architecture of the buildings, pits and silos has been correlated with the artefact data and the degree of proximity between the structures. In spite of the low number of artefacts found for each of the five internal chronological stages defined by the pottery and a complex horizontal stratigraphy we have attempted to perceive the elementary composition of the eleven rural domestic units thus identified; their probable surfaces -
without the peripheral silos - vary between approximately 3000 square
metres for the smallest (and earliest) and 1.2 hectares for the largest (and most recent).
Les éléments de parure en verre du site de Lumaca (Âge du Fer, Centuri, Haute-Corse) : compositions et typochronologie
Authors: Gratuze Bernard , Lorenzi Françoise 
Published: May 2010

Lumaca, an open air settlement located at the northern end of Cap Corse, was occupied from the Early Neolithic until historic times. This long occupation is explained by the presence of a little sheepfold; all the stratigraphic levels are strongly disturbed. During the excavation of the site eight glass beads and rings were discovered. Analyses reveal that these ancient glass beads were all manufactured with Near-Eastern soda glasses. These glasses, obtained by fusing calcareous sands together with a mineral
soda flux, characterise the production of the Syro-Palestinian glass
factories and are found throughout the Mediterranean Basin from the
beginning of the first millennium B. C. to the end of the first millennium
A. D. The analytical data allow the beads from Lumaca to be divided in three groups which are essentially attributed to the Iron Ages (from Hallstatt to the 1st century A. D.). It is important to note that these beads were discovered in a dwelling and not (as often) in a tomb.
Sobre el origen de los posesivos tuyo y suyo
Authors: Romero Cambrón Àngeles 
Published: May 2010

Ce travail aborde l’étude historique des nouveaux possessifs de deuxième et de troisième personne du singulier «tuyo» et «suyo». Ces formes remplacent les anciennes «to», «o», «túa» et
«súa» en tant qu’attributs («es suyo»), comme adjectifs précédés de l’article («lo suyo»), comme prédicatifs avec préposition («por suyo») et placés après le substantif («fi jo suyo»). L’objectif est de passer en revue les premiers documents où apparaissent ces possessifs, de faire un état des lieux de leur implantation dans les anciens dialectes (ils sont présents en castillan et en aragonais, mais pas en léonais), ainsi que d’examiner, à l’aide de nouveaux arguments, les deux hypothèses qui sont utilisées traditionnellement pour expliquer leur origine: la phonétique, qui recourt au développement d’une consonne anti-hiatique et, plus précisément, l’hypothèse analogique, qui suppose l’influence du relatif et interrogatif «cuyo».
Le contact forêt-savane. Rôle des régimes hydriques des sols dans l'Ouest de la Côte-d'Ivoire
Authors: Avenard Jean-Michel 
Published: May 2010

The boundary between forest and savanna. The role of water-flows in western ivory coast.

Because of historical factors, physical geography has long been too much analytic. Thus it could not join in the birth of vegetal ecology, which is botanists' achievement. The development of climatic geomorphology enabled to show another trend. It constitutes a first integrating element of the different divergent branches of physical geography.
J.-M. Avenard's studies in the Ivory Coast go farther in that promising way. They are about bio geographical subject : the boundary between savanna and forest and they tend to determine some ecological conditions. The essential factor is the water-flow of soils, for the forest is more demanding than the savanna. But the processes are not simple. On one hand there are the water reserves of the soil which help the forest to endure an already long and intensive dry season. But on the other hand tree-roots cannot develop in a congested and asphyxiating soil, which expels the forest from some inundable plains. Now the water-flow in soils depends largely on many other physico-geographical elements : above all the climate, the geomorphological evolution, the lithology and the pedo genesis. J.-M. Avenard mastered this problem very well for he could exactly measure soil-humidity and he brought in many data which explain its variation as a part of the whole physical environment. His article is at the same time, according to the new orientation we give to the Annales de Géographie, a statement on an important problem and a method demonstration.
Sémiotique grammaticale et sémantique des (genres de) jeux de langage : les « pronoms personnels » clitiques en français
Authors: Bouquet Simon 
Published: May 2010

Simon Bouquet: Semiotics of grammar and langage game ("genres") semantics: the personal pronouns in French.
This paper proposes an epistemological framework to fulfil the saussurean program of a dualist langage science including "linguistique de la langue" and "linguistique de la parole". The semantic analysis - founded both on Peirce's semiotics (grammar/"langue") and on Wittgenstein's theory of langage game ("parole") - is exemplified by the paradigm of personal pronouns in French.
Trois perspectives linguistiques sur la notion de genre discursif
Authors: Beacco Jean-Claude 
Published: May 2010

Jean-Claude Beacco : Trois perspectives linguistiques sur la notion de genre discursif.
This contribution examines how a complex entity as "genres" can be described in linguistic terms. It is assumed that genres are ordinary categories through which communication is analysed in speech communities and which organises everyday communication: we speak through genres, as M. Bakhtine used to write. In that sense, the study of genres (specially of the genres names) is part of folk linguistics. Genres can also be described as text types, which shows common features in structure, morpho-syntaxic realisations, tone, theme... from a comparative point of view and using the categories of text linguistics. Genres are also part of institutions and expression of social and ideological conflicts: this socio-historical dimension could explain some of their formal and semantic features in the theoretical framework of discourse analysis. It is argued that these three forms of linguistic approaches of genres are together necessary to their description.
Poétique et textualité
Authors: Rastier François 
Published: May 2010

François Rastier : Poétique et textualité.
Despite some reluctance, the problem of genres is part and parcel of linguistics. Genre is a global clue which characterizes every text. Moreover, genre determines the relationship between the two strata of language at the text level, by means of what we may call textual semiosis. Genres also have a feedback on local clues, at the level of vocabulary and morphosyntax. Finally, the description of genres is necessary to conceive the space of norms, a mediation between a linguistic system and usage.