bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of Quercetin Biosensor Through Immobilizing Laccase in a Modified β‐Cyclodextrin Matrix Containing Ag Nanoparticles in Ionic Liquid
Authors: Franzoi Ana Cristina , Cruz Vieira Iolanda , Weber Scheeren Carla , Dupont Jairton 
Published: April 2010

AbstractA biosensor containing Ag nanoparticles in ionic liquid (IL) 1‐butyl‐3‐methylimidazolium bis(trifluoromethylsulfonyl)imide (BMI.Tf2N) and laccase (Lac) immobilized on β‐cyclodextrin modified with epichlorohydrin (β‐CDEpi) was developed for quercetin determination. Lac catalyses the oxidation of quercetin to quinone, which is then reduced on the biosensor surface and the resulting current was investigated by square‐wave voltammetry. The β‐CDEpi support was analyzed by scanning electron microscopy and Ag‐BMI.Tf2N by transmission electron microscopy and X‐ray diffraction. The linear range (0.499–7.407 μM) and low detection limit (0.037±0.004 μM) show that the proposed biosensor is suitable for quercetin determination in real samples.
Amperometric Determination of Glucose at Conventional vs. Nanostructured Gold Electrodes in Neutral Solutions
Authors: Wooten Marilyn , Shim Jun Ho , Gorski Waldemar 
Published: April 2010

AbstractThe conventional gold electrodes were compared with recently published electrodes based on gold nanoparticles and gold nanostructured films as amperometric sensors for glucose in pH 7.40 phosphate buffer solutions. The conventional electrodes provided similar electroanalytical benefits while required much simpler and shorter preparation. It is recommended that the future reports on the development of electrochemical sensors based on metal nanoparticles/nanostructures include also the analytical figures of merit obtained at relevant conventional metal electrodes. The voltammetric studies indicated that, in contrast to phosphate buffers, the Tris buffers were not suitable for activation of gold surface toward the direct oxidation of glucose.
Exercise‐induced lipid peroxidation: Implications for deoxyribonucleic acid damage and systemic free radical generation
Authors: Fogarty Mark C. , Hughes Ciara M. , Burke George , Brown John C. , Trinick Tom R. , Duly Ellie , Bailey Damian M. , Davison Gareth W. 
Published: April 2010

AbstractExercise‐induced deoxyribonucleic acid (DNA) damage is often associated with an increase in free radicals; however, there is a lack of evidence examining the two in parallel. This study tested the hypothesis that high‐intensity exercise has the ability to produce free radicals that may be capable of causing DNA damage. Twelve apparently healthy male subjects (age: 23 ± 4 years; stature: 181 ± 8 cm; body mass: 80 ± 9 kg; and VO2max: 49 ± 5 ml/kg/min) performed three 5 min consecutive and incremental stages (40, 70, and 100% of VO2max) of aerobic exercise with a 15‐min period separating each stage. Blood was drawn after each bout of exercise for the determination of ex vivo free radicals, DNA damage, protein carbonyls, lipid hydroperoxide (LOOH) concentration, and a range of lipid‐soluble antioxidants. Lipid‐derived oxygen‐centered free radicals (hyperfine coupling constants aNitrogen = 13.7 Gauss (G) and aβHydrogen = 1.8 G) increased as a result of acute moderate and high‐intensity exercise (P < 0.05), while DNA damage was also increased (P < 0.05). Systemic changes were observed in LOOH and for lipid‐soluble antioxidants throughout exercise (P < 0.05); however, there was no observed change in protein carbonyl concentration (P > 0.05). These findings identify lipid‐derived free radical species as possible contributors to peripheral mononuclear cell DNA damage in the human exercising model. This damage occurs in the presence of lipid oxidation but in the absence of any change to protein carbonyl concentration. The significance of these findings may have relevance in terms of immune function, the aging process, and the pathology of carcinogenesis. Environ. Mol. Mutagen. 52:35–42, 2011. © 2010 Wiley‐Liss, Inc.
DNA repair in mammalian mitochondria: Much more than we thought?
Authors: Liu Pingfang , Demple Bruce 
Published: April 2010

AbstractFor many years, the repair of most damage in mitochondrial DNA (mtDNA) was thought limited to short‐patch base excision repair (SP‐BER), which replaces a single nucleotide by the sequential action of DNA glycosylases, an apurinic/apyrimidinic (AP) endonuclease, the mitochondrial DNA polymerase γ, an abasic lyase activity, and mitochondrial DNA ligase. However, the likely array of lesions inflicted on mtDNA by oxygen radicals and the possibility of replication errors and disruptions indicated that such a restricted repair repertoire would be inadequate. Recent studies have considerably expanded our knowledge of mtDNA repair to include long‐patch base excision repair (LP‐BER), mismatch repair, and homologous recombination and nonhomologous end‐joining. In addition, elimination of mutagenic 8‐oxodeoxyguanosine triphosphate (8‐oxodGTP) helps prevent cell death due to the accumulation of this oxidation product in mtDNA. Although it was suspected for many years that irreparably damaged mtDNA might be targeted for degradation, only recently was clear evidence provided for this hypothesis. Therefore, multiple DNA repair pathways and controlled degradation of mtDNA function together to maintain the integrity of mitochondrial genome. Environ. Mol. Mutagen., 2010. © 2010 Wiley‐Liss, Inc.
Effects of in ovo exposure of white leghorn chicken, common pheasant, and Japanese quail to 2,3,7,8-tetrachlorodibenzo-p-dioxin and two chlorinated dibenzofurans on CYP1A induction
Authors: Yang Yinfei , Wiseman Steve , Cohen-Barnhouse Andrew M. , Wan Yi , Jones Paul D. , Newsted John L. , Kay Denise P. , Kennedy Sean W. , Zwiernik Matthew J. , Bursian Steven J. , Giesy John P. 
Published: April 2010

AbstractIn birds, activation of the aryl hydrocarbon receptor (AhR) by some polychlorinated dibenzo-p-dioxins (PCDDs) and polychlorinated dibenzofurans (PCDFs) results in induction of cytochrome P4501A (CYP1A) expression. This response has been useful for predicting relative sensitivity of birds to dioxin-like compounds. To further investigate species-sensitivity to dioxins and dioxin-like compounds induction of cytochrome P450 1A4 and 1A5 (CYP1A4 and CYP1A5) mRNA and ethoxyresorufin O-deethylase (EROD) activity were quantified in liver of posthatch white leghorn chicken, common pheasant, and Japanese quail exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,4,7,8-pentachlorodibenzofuran (PeCDF), or 2,3,7,8-tetrachlorodibenzofuran (TCDF) via air cell injection. The rank-order of sensitivity of TCDD- and TCDF-exposed birds, based on CYP1A, was chicken &gt; pheasant &gt; quail. Based on CYP1A5 mRNA expression and EROD induction, the order of sensitivity of PeCDF-exposed birds was identical to that for TCDD and TCDF. However, based on CYP1A4 mRNA expression the rank-order was pheasant &gt; chicken &gt; quail. When comparing the potency of the three compounds in each species, based on CYP1A4 mRNA expression, TCDD was the most potent compound in chicken. However, PeCDF was equally potent to TCDD in quail and was more potent than TCDD in pheasant. These results suggest that quantitative real-time polymerase chain reaction (Q-PCR) analysis of CYP1A expression, particularly CYP1A4 mRNA expression, may be a more sensitive biomarker of exposure than analysis of EROD induction, especially in less responsive avian species. Based on these findings future risk assessments should consider the sensitivity of the species inhabiting a site and the congeners of concern that are present. Environ. Toxicol. Chem. 2010;29:1490–1502. © 2010 SETAC
Bioavailability of hexabromocyclododecane to the polychaeteHediste diversicolor: Exposure through sediment and food from a contaminated fjord
Authors: Haukås Marianne , Ruus Anders , Hylland Ketil , Berge John Arthur , Mariussen Espen 
Published: April 2010

AbstractKnowledge of the bioavailability of brominated flame retardants for sediment-dwelling organisms is limited. The present study investigated bioaccumulation of hexabromocyclododecane (HBCD) in the ragworm Hediste diversicolor exposed to field-contaminated sediments and food. Sediments and blue mussels (Mytilus edulis) were sampled from a fjord on the Norwegian west coast known to be highly contaminated with the brominated flame retardant HBCD. During a four-week experiment, ragworms were either exposed to sediment containing moderate or high concentrations of HBCD, or they were fed contaminated soft tissue from mussel. Although mean sediment concentrations were as high as 40 µg/g dry weight in the contaminated sediments, no HBCD were detected in the exposed ragworms (detection limit up to 2.0 ng/g wet wt). In contrast, the exposure to contaminated food (mussel) resulted in elevated body concentrations of 9.1 ng HBCD/g wet weight. The partitioning of HBCD in the test-sediments appeared to be influenced by the quantity and quality of the sediment organic matter (up to 15% total organic carbon). The presence of small plastic beads (&lt;2 mm), which were shown to contain a mean of 50 µg/g dry weight of HBCD, could explain the low bioavailability of the flame retardant in the sediment. Hexabromocyclododecane in food appeared to be readily assimilated in the ragworm, however, and the diastereomer pattern indicated a relative increase of α-HBCD from mussel to ragworm. Hence, in the sampled areas of the fjord, trophic transfer appeared to be a more important mechanism for the entry of HBCD into the local benthic food web than sediment-to-biota accumulation. Environ. Toxicol. Chem. 2010; 29:1709–1715. © 2010 SETAC
Autoantibodies against stress‐induced phosphoprotein‐1 as a novel biomarker candidate for ovarian cancer
Authors: Kim Sunghoon , Cho HanByoul , Nam Eun Ji , Kim Sang Wun , Kim Young Tae , Park Yong Won , Kim Bo Wook , Kim Jae‐Hoon 
Published: April 2010

AbstractDetection of autoantibodies against tumor‐associated antigens (TAA) has recently been shown to be a powerful tool for early detection of various cancers. The aim of this study was to investigate the possibility of using autoantibodies against TAA as novel biomarkers by a proteomics‐based approach in patients with ovarian cancer. We used two‐dimensional differential gel electrophoresis analysis of immuno‐precipitated tumor antigens (2D‐DITA) to compare the levels of autoandibodies in pretreatment and posttreatment sera of patients with ovarian cancers. The identified autoantibodies were validated by SYBR Green real‐time polymerase chain reaction (PCR) and immunohistochemistry (IHC). We further evaluated the level of autoantibody in sera of 68 ovarian cancer patients by an enzyme‐linked immunosorbent assay (ELISA). The autoantibody directed against stress‐induced phosphoprotein‐1 (STIP‐1) emerged as a novel biomarker candidate for ovarian cancer. SYBR Green PCR and IHC confirmed that the STIP‐1 mRNA and protein expression levels were significantly up‐regulated in ovarian cancers compared with normal and benign tumors (P = 0.003 and P < 0.001, respectively). A preliminary ELISA study showed that the serum levels of anti‐STIP‐1 autoantibodies were significantly elevated in ovarian cancer patients compared with healthy controls (P = 0.03). The results suggest that 2D‐DITA is a useful tool to detect autoantibodies and that STIP‐1 is a potential biomarker candidate for ovarian cancers. © 2010 Wiley‐Liss, Inc.
Genome‐wide analysis of allelic imbalances reveals 4q deletions as a poor prognostic factor and MDM4 amplification at 1q32.1 in hepatoblastoma
Authors: Arai Yasuhito , Honda Shohei , Haruta Masayuki , Kasai Fumio , Fujiwara Yuiko , Ohshima Junjiro , Sasaki Fumiaki , Nakagawara Akira , Horie Hiroshi , Yamaoka Hiroyuki , Hiyama Eiso , Kaneko Yasuhiko 
Published: April 2010

AbstractIn a single‐nucleotide polymorphism array‐based analysis of 56 hepatoblastoma (HB) tumors, allelic imbalances were detected in 37 tumors (66%). Chromosome gains were found in 1q (28 tumors), 2q (24), 6p (8), 8q (8), 17q (6), and 20pq (10), and losses in 1p (6), 4q (9), and 16q (4). Fine mapping delineated the shortest overlapping region (SOR) of gains at 1q32.1 (1.3 Mb) and 2q24.2‐q24.3 (4.8 Mb), and losses at 4q34.3‐q35.2 (8.7 Mb) and 4q32.3 (1.6 Mb). Uniparental disomy of 11pter‐11p15.4 (IGF2) and loss of 11pter‐p14.1 were found in 11 and 2 tumors, respectively. Expression of HTATIP2 (11p15.1) was absent in 9 of 20 tumors. Amplification was identified in four tumors at 1q32.1, where the candidate oncogene MDM4 is located. In the 4q32.3‐SRO, ANXA10S, a variant of the candidate tumor suppressor ANXA10, showed no expression in 19 of 24 tumors. Sequence analysis of ANXA10S identified a missense mutation (E36K, c.106G>A) in a HB cell line. Multivariate analysis revealed that both 4q deletion and RASSF1A methylation (relative risks: 4.21 and 7.55, respectively) are independent prognostic factors. Our results indicate that allelic imbalances and gene expression patterns provide possible diagnostic and prognostic markers, as well as therapeutic targets in a subset of HB. © 2010 Wiley‐Liss, Inc.
Combined classical cytogenetics and microarray‐based genomic copy number analysis reveal frequent 3;5 rearrangements in clear cell renal cell carcinoma
Authors: Pei Jianming , Feder Madelyn M. , Al‐Saleem Tahseen , Liu Zemin , Liu Angen , Hudes Gary R. , Uzzo Robert G. , Testa Joseph R. 
Published: April 2010

AbstractKaryotypic analysis and genomic copy number analysis with single nucleotide polymorphism (SNP)‐based microarrays were compared with regard to the detection of recurrent genomic imbalances in 20 clear cell renal cell carcinomas (ccRCCs). Genomic imbalances were identified in 19 of 20 tumors by DNA copy number analysis and in 15 tumors by classical cytogenetics. A statistically significant correlation was observed between the number of genomic imbalances and tumor stage. The most common genomic imbalances were loss of 3p and gain of 5q. Other recurrent genomic imbalances seen in at least 15% of tumors included losses of 1p32.3‐p33, 6q23.1‐qter and 14q and gain of chromosome 7. The SNP‐based arrays revealed losses of 3p in 16 of 20 tumors, with the highest frequency being at 3p21.31‐p22.1 and 3p24.3‐p25.3, the latter encompassing the VHL locus. One other tumor showed uniparental disomy of chromosome 3. Thus, altogether loss of 3p was identified in 17 of 20 (85%) cases. Fourteen tumors showed both overlapping losses of 3p and overlapping gains of 5q, and the karyotypic assessment performed in parallel revealed that these imbalances arose via unbalanced 3;5 translocations. Among the latter, there were common regions of loss at 3p21.3‐pter and gain at 5q34‐qter. These data suggest that DNA copy number analysis will supplant karyotypic analysis of tumor types such as ccRCC that are characterized by recurrent genomic imbalances, rather than balanced rearrangements. These findings also suggest that the 5q duplication/3p deficiency resulting from unbalanced 3;5 translocations conveys a proliferative advantage of particular importance in ccRCC tumorigenesis. © 2010 Wiley‐Liss, Inc.
HMGA1 and HMGA2 rearrangements in mass‐forming endometriosis
Authors: Medeiros Fabiola , Araujo Ana R. , Erickson‐Johnson Michele R. , Kashyap Purna C. , Dal Cin Paola , Nucci Marisa , Wang Xiaoke , Bell Debra A. , Oliveira Andre M. 
Published: April 2010

AbstractEndometriosis is a common gynecologic disorder characterized by ectopic endometrium associated with pelvic pain and infertility. The pathogenesis of endometriosis is unclear, and several genetic, endocrine, immune, and environmental agents have been studied as putative causative factors. However, consistent somatic genetic alterations have not been identified. Rarely, endometriosis presents as a mass lesion with an infiltrative pattern reminiscent of malignancy. We describe cytogenetic and molecular cytogenetic findings of mass‐forming endometriosis. The index case of pulmonary endometriosis underwent conventional and molecular cytogenetics analysis. In addition, 16 cases of mass‐forming endometriosis, 11 cases of usual endometriosis, and six endometriomas were investigated by fluorescence in situ hybridization (FISH) for HMGA1 and HMGA2 loci, performed on paraffin‐embedded thin tissue sections with custom‐designed probes. The index patient had an endometriotic lung nodule, with a 46,XX, t(5;6)(q13;p21) karyotype and HMGA1 rearrangement by FISH. A second patient had decidualized endometriosis forming a large abdominal mass and HMGA1 rearrangement by FISH. Of the 15 other cases of mass‐forming endometriosis, one had HMGA1 rearrangement and two had HMGA2 rearrangement. The rearrangements were found in the stromal component exclusively. None of the usual endometriosis cases or endometriomas had HMGA1 or HMGA2 rearrangements. In conclusion, mass‐forming endometriosis is an uncommon subset of endometriosis that harbors HMGA1 or HMGA2 rearrangements in up to 29% of cases. The present findings support the concept that endometriosis is clonal and that rearrangement of HMGA genes likely contributes to its pathogenesis. © 2010 Wiley‐Liss, Inc.
Late Ordovician (Sandbian) brachiopods from the Mweelrea Formation, South Mayo, western Ireland: stratigraphic and tectonic implications
Authors: Harper David A. T. , Parkes Matthew A. , McConnell Brian J. 
Published: April 2010

AbstractA Late Ordovician (Sandbian), Scoto‐Appalachian brachiopod fauna from the Mweelrea Formation in western Ireland confirms a location for the South Mayo Trough adjacent to the Laurentian margin, characterized at this time by a succession of marine excursions over fluviatile environments. The new, younger biostratigraphical data help to constrain the timing of late Grampian folding of this part of the South Mayo Trough. Copyright © 2010 John Wiley & Sons, Ltd.
Implicit memory for object locations depends on reactivation of encoding‐related brain regions
Authors: Manelis Anna , Hanson Catherine , Hanson Stephen José 
Published: April 2010

AbstractThis study explored the correspondence between implicit memory and the reactivation of encoding‐related brain regions. By using a classification method, we examined whether reactivation reflects only the similarities between study and test or voxels at the reactivated regions are diagnostic of facilitation in the implicit memory task. A simple detection task served as incidental encoding of object–location pairings. A subsequent visual search task served as the indirect (implicit) test of memory. Subjects did not know that their memory would be tested. Half of the subjects were unaware that some stimuli in the search task are the same as those that had appeared during the detection task. Another group of subjects was made aware of this relationship at the onset of the visual search task. Memory performance was superior for the study‐test aware, compared to study‐test unaware, subjects. Brain reactivation was calculated using a conjunction analysis implemented through overlaying the neural activity at encoding and testing. The conjunction analysis revealed that implicit memory in both groups of subjects was associated with reactivation of parietal and occipital brain regions. We were able to classify study‐test aware and study‐test unaware subjects based on the per‐voxel reactivation values representing the neural dynamics between encoding and test. The classification results indicate that neural dynamics between encoding and test accounts for the differences in implicit memory. Overall, our study demonstrates that implicit memory performance requires and depends upon reactivation of encoding‐related brain regions. Hum Brain Mapp, 2010. © 2010 Wiley‐Liss, Inc.
Diffusion tensor‐based regional gray matter tissue segmentation using the international consortium for brain mapping atlases
Authors: Hasan Khader M. , Frye Richard E. 
Published: April 2010

AbstractIn this communication, we extended a previously described and validated diffusion tensor imaging (DTI) method for segmenting whole brain cerebrospinal fluid (CSF) and gray and white matter (WM) tissue to provide regional volume and DTI metrics of WM tract and cortical and subcortical gray matter. This DTI‐based regional segmentation was implemented using the statistical parametric mapping (SPM) toolbox and used the international consortium for brain mapping atlases and Montreal Neurological Institute brain templates. We used our DTI‐based segmentation approach to calculate the left putamen volume in a cohort of 136 healthy right‐handed males and females aged 15.8–62.8 years. We validated our approach by demonstrating its sensitivity to age‐related changes of the putamen. Indeed, our method found that the putamen volume decreased with age (r= −0.30;P< 0.001) while the corresponding fractional anisotropy (FA) increased with advancing age (r= 0.5;P< 0.00001). It is then demonstrated, on a subset of our cohort (n= 31), that the putamen volume obtained by our method correlated with measurements obtained from FreeSurfer (r= 0.396,P< 0.05). Our novel approach increases the information obtained with a DTI examination by providing routine volumetry measure, thereby eliminating separate scans to obtain volumetry data. In addition, the labeled volumes obtained with our method have the potential to increase the accuracy of fiber tracking. In the future, this new approach can be automated to analyze large data sets to help discover noninvasive neuroimaging markers for clinical trials and brain‐function studies in both health and disease. Hum Brain Mapp, 2010. © 2010 Wiley‐Liss, Inc.
Low‐frequency stimulation of the temporoammonic pathway induces heterosynaptic disinhibition in the subiculum
Authors: Fidzinski Pawel , Wawra Matthias , Dugladze Tamar , Gloveli Tengis , Heinemann Uwe , Behr Joachim 
Published: April 2010

AbstractThe subiculum (Sub) is the principal target of CA1 pyramidal cells. It serves as the final relay of hippocampal output and thus mediates hippocampal–cortical interaction. In addition, the Sub receives direct input from the entorhinal cortex via the temporoammonic pathway. In this study, we demonstrate that low‐frequency stimulation of the temporoammonic pathway results in the disinhibition of excitatory synaptic transmission at CA1‐Sub synapses. We provide evidence that this disinhibition is mediated by an NMDA receptor‐dependent long‐term depression (LTD) of GABAergic inhibition. This mechanism might bear physiological significance for the stabilization and processing of mnemonic information at hippocampal output synapses and underpins the functional role of hippocampal–entorhinal interaction in memory formation. © 2010 Wiley‐Liss, Inc.
Measurement of effective oxygen diffusivity in microporous media containing moisture
Authors: Utaka Yoshio , Iwasaki Daigo , Tasaki Yutaka , Wang Shixue 
Published: April 2010

AbstractThe mass transfer characteristics of the gas diffusion layer (GDL) are closely related to the cell performance of a polymer electrolyte fuel cell (PEFC). The oxygen diffusivity of paper type porous media, which is generally used as a GDL, was measured with respect to its liquid water content using experimental apparatus consisting of an oxygen sensor based on the galvanic cell. A numerical method was established to obtain the effective oxygen diffusivity of microporous test materials by calculating the oxygen concentration distribution on both sides of the test material. Experimental results indicate that the relative oxygen diffusivity of paper type GDLs increases nonlinearly as the water saturation decreases. © 2010 Wiley Periodicals, Inc. Heat Trans Asian Res; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/htj.20295