bluuebunny/crossref_metadata_2025_split huggingface dataset:
A simple, selective and rapid validated method for estimation of anastrazole in human plasma by liquid chromatography–tandem mass spectrometry and its application to bioequivalence study
Authors: Jangid Arvind G. , Pudage Ashutosh M. , Joshi Santosh S. , Pabrekar Pramod N. , Tale Rajesh H. , Vaidya Vikas V. 
Published: November 2009

AbstractA rapid, simple and specific method for estimation of anastrazole in human plasma was validated using letrozole as internal standard. The analyte and internal standard were extracted from plasma using simple solid‐phase extraction. The compound were separated on a reverse‐phase column with an isocratic mobile phase consisting of 0.1% formic acid in water and acetonitrile (12 : 88, v/v) and detected by tandem mass spectrometry in positive ion mode. The ion transitions recorded in multiple reaction monitoring mode were m/z 294.1 → 225.1 for anastrazole and m/z 286.1 → 217.1 for internal standard. Linearity in plasma was observed over the concentration range 0.3–30 ng/mL for anastrazole. The mean recovery for anastrazole was 83.7% with a lower limit of quantification of 0.3 ng/mL. The coefficient of variation of the assay was less than 6.8% and the accuracy was 96.1–102.2%. The validated method was applied to a bioequivalence study of 1 mg anastrazole tablet in healthy human volunteers. Copyright © 2009 John Wiley & Sons, Ltd.
Liquid chromatography–mass spectrometry methods for urinary biomarker detection in metabonomic studies with application to nutritional studies
Authors: Legido‐Quigley Cristina , Stella Cinzia , Perez‐Jimenez Francisco , Lopez‐Miranda Jose , Ordovas Jose , Powell Jonathan , van‐der‐Ouderaa Frans , Ware Lisa , Lindon John C. , Nicholson Jeremy K. , Holmes Elaine 
Published: November 2009

AbstractThe effects of sample preparation and chromatographic method differences on the classification and recovery of metabolic biomarkers from UPLC‐MS measurements on urine samples of humans exposed to different dietary interventions have been investigated. Eight volunteers consumed three high‐fat meals (rich in saturated, monounsaturated and polyunsaturated fatty acids, respectively) in randomized order with a washout period in between. For each participant, urine samples were obtained prior to and at three timed intervals after each meal. Samples were processed either by dilution (1 : 4) or by liquid–liquid extraction and then run under two different gradient conditions. For each analysis method, a total of 96 observations (eight participants, four time points, three diets) were measured. The total ion count chromatograms were analyzed using partial‐least‐squares discriminant analysis. All three dietary classes could be discriminated irrespective of sample preparation and chromatographic method. However, the main discriminating metabolites varied according to sample preparation, indicating that sample treatment and chromatographic conditions influence the ability to extract biomolecular information. Diluted samples showed higher m/z compounds (ca 400 u) while liquid–liquid extraction samples showed low m/z at the same retention time span. Optimized methods for metabolite identification (e.g. organic acids) were statistically inferior to global screening for mixed compound identification, confirming that multiple compound class‐based metabolic profiles are likely to give superior metabonomic (diagnostic) classification, although great care has to be taken in the interpretation in relation to matrix effects. Copyright © 2009 John Wiley & Sons, Ltd.
Quantitative sphingosine measurement as a surrogate for total ceramide concentration—preclinical and potential translational applications
Authors: Kindt Erick , Wetterau John , Mueller Sandra Bak , Castle Christine , Boustany‐Kari Carine M. 
Published: November 2009

AbstractBiomarkers are an increasingly important constituent of the drug development process, offering the potential of increased efficiency through reduced compound attrition and earlier proof of mechanism and/or efficacy. Assays developed for compound screening that can be directly translated for clinical trials are especially valuable, but their successful adoption requires a careful balance between assay performance and implementation costs. One such ‘fit‐for‐purpose’ biomarker assay, the indirect measurement of pharmacological modulation of sphingolipid biosynthesis and disposition, is presented here. Among spingolipids, numerous ceramide species are readily detectable in different lipoprotein fractions of mammalian plasma, but their parallel quantification can be prohibitively expensive and time consuming. Ceramides differ in their fatty acid moiety, which is readily removed by hydrolysis, yielding a common sphingosine derivative, the measurement of which serves as an indicator of total ceramide. When followed by liquid chromatography tandem mass spectrometry (LC/MS/MS) for detection, robust analyte quantification becomes relatively straightforward. The practical utility of a method developed to be fit for the purpose of rapidly and quantitatively measuring treatment‐induced variations in total ceramide from hamster plasma and individual lipoprotein fractions is described. With a linear calibration range from 0.003 to 33.4 μm sphingosine, precision and accuracy error in plasma‐based quality controls spiked with ceramides was less than 15%. The specificity of the assay for ceramides was also assessed. The simplicity of the method would allow for its potential translation to other preclinical species, as well as for clinical applications in later‐stage drug development. Copyright © 2009 John Wiley & Sons, Ltd.
Risk communication in sexually violent predator hearings
Authors: Scott Sarah , Gilcrist Brett , Thurston Nicole , Huss Matthew T. 
Published: November 2009

AbstractSexually violent predator (SVP) laws use the civil commitment process to confine mentally disordered and dangerous offenders who are at high risk to reoffend. Few studies have examined how jurors decide SVP cases. As a result, a pilot study and three experimental studies were conducted, in which victim type, risk communication, and juror education were manipulated to assess juror response. Results continually illustrated that victim type was the most salient manipulation across studies and that the manner of risk communication and juror education had little impact on jurors. Copyright © 2009 John Wiley & Sons, Ltd.
Apoptosis‐inducing effects of Morinda citrifolia L. and doxorubicin on the Ehrlich ascites tumor in Balb‐c mice
Authors: Taşkın Elif İlkay , Akgün‐Dar Kadriye , Kapucu Ayşegül , Osanç Esma , Doğruman Hüsniye , Eraltan Hakan , Ulukaya Engin 
Published: November 2009

AbstractMorinda citrifolia L. (Noni) is a herbal remedy with promising anti‐cancer properties. However, its effects on various cancers are to be investigated to make a firm conclusion before implementing it into the clinical practice. Therefore, we investigated the cytotoxic potential of noni on Ehrlich ascites tumor grown in female Balb‐c mice and also combined it with a potent anti‐cancer agent, doxorubicin. One group received noni only (n = 8), another one doxorubicin (n = 8), and the other one noni + doxorubicin (n = 8) for 14 days after the inoculation of cells. The control group (n = 7) received 0.9% NaCl only. We found that short and long diameters of the tumor tissues were about 40–50% smaller, compared to those in control group. This anti‐growth effect resulted from the induction of apoptosis, which was confirmed by the positive results from the Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL) analysis and the active caspase‐3 cells in tissues. Apoptosis also confirmed by caspase‐cleaved cytokeratin 18 elevation in serum of the treated groups. Further, the proliferation was decreased, which was immunohistochemically shown by the PCNA staining. We conclude that noni may be useful in the treatment of breast cancer either on its own or in combination with doxorubicin. Further studies are warranted to assess the dosage and safety of using noni fruit juice in conjuction with anti‐cancer drugs against breast cancer. Copyright © 2009 John Wiley & Sons, Ltd.
The role of postoperative radiotherapy for the treatment of gangliogliomas
Authors: Rades Dirk , Zwick Lena , Leppert Jan , Bonsanto Matteo M , Tronnier Volker , Dunst Juergen , Schild Steven E. 
Published: November 2009

AbstractBACKGROUND:Because of their rarity, no prospective studies have been performed regarding gangliogliomas. The optimal treatment regimen is unclear. In this study, the authors compared 4 therapies for local control (LC) and overall survival (OS) in patients with ganglioglioma.METHODS:In 402 patients with ganglioglioma, outcomes were compared for patients who underwent gross total resection alone (GTR) (n = 188), GTR plus radiotherapy (GTR + RT) (n = 21), subtotal resection alone (STR) (n = 113), and STR plus RT (STR + RT (n = 80). Age, sex, tumor site, and histologic grade also were investigated. Subgroup analyses were performed for both low‐grade and high‐grade tumors.RESULTS:The 10‐year LC rates were 89% after GTR, 90% after GTR + RT, 52% after STR, and 65% after STR + RT (P < .001); and the 10‐year OS rates were 95%, 95%, 62%, and 74%, respectively (P < .001). After STR, irradiation significantly improved LC (P = .004) but not OS (P = .22). After GTR, irradiation did not significantly improve LC (P = .23) or OS (P = .29). On multivariate analyses, LC and OS were associated with therapy and pathologic grade, and OS also was associated with tumor site. In low‐grade tumors, STR + RT resulted in better LC (P = .016) but not better OS (P = .18); and, after GTR, LC (P = .28) and OS (P = 1.0) were not improved with postoperative radiotherapy. In high‐grade tumors, STR + RT resulted in better LC (P = .016) but not better OS (P = .41); after GTR, LC (P = .56) and OS (P = .61) were not improved with irradiation.CONCLUSIONS:According to this review, GTR should be performed whenever safely possible and does not require postoperative irradiation. If only STR is achieved, then RT improves LC of both low‐grade and high‐grade tumors and, thus, should be considered seriously. Cancer 2010. © 2010 American Cancer Society.
Treatment patterns of aging Americans with differentiated thyroid cancer
Authors: Park Henry S. , Roman Sanziana A. , Sosa Julie Ann 
Published: November 2009

AbstractBACKGROUND:The incidence of differentiated thyroid cancer (DTC) increases with age. Total thyroidectomy, often followed by radioactive iodine (RAI), is recommended for patients who have tumors that measure ≥1 cm in greatest dimension. In the current study, the authors assessed the use of thyroidectomy and RAI among elderly patients with DTC and the effects on survival.METHODS:Adults aged ≥45 years with DTC ≥1 cm in the Surveillance, Epidemiology, and End Results database from 1988 to 2003 were included. Bivariate and multivariate analyses were used to measure associations between demographic, clinical, and pathologic characteristics and the likelihood of receiving treatment according to current practice guidelines.RESULTS:Of 8899 patients who were identified, 26% were ages 65 years to 79 years, and 5% were aged ≥80 years. Compared with younger patients, patients aged ≥ 65 years were more likely to have larger tumors, stage IV disease, extrathyroid extension, and nonpapillary histology. Elderly patients were less likely to undergo total thyroidectomy (74% vs 80%; P < .001) or to receive RAI (47% vs 54%; P < .001). These trends were most pronounced among those aged ≥80 years. Among the patients who did not undergo surgery, elderly patients did not report higher rates of contraindications to surgery. In multivariate analysis, the groups ages 65 years to 79 years and aged ≥80 years were associated with lower rates of total thyroidectomy (odds ratio, 0.77 and 0.43, respectively; P < .001) and RAI (odds ratio, 0.85 [P < .01] and 0.39 [P < .001], respectively). Among elderly patients, predictors of worse survival included no surgery (hazard ratio, 5.51; P < .001) and no RAI (hazard ratio, 1.36; P < .001).CONCLUSIONS:Elderly patients with DTC received less aggressive surgical and RAI treatment than younger patients despite having more advanced disease and the improved survival associated with these treatments among elderly patients. Long‐term outcomes should be measured to determine the impact of this apparent discrepancy in care. Cancer 2010. © 2010 American Cancer Society.
Health status of the oldest adult survivors of cancer during childhood
Authors: Kenney Lisa B , Nancarrow Cheryl Medeiros , Najita Julie , Vrooman Lynda M. , Rothwell Monica , Recklitis Christopher , Li Frederick P. , Diller Lisa 
Published: November 2009

AbstractBACKGROUND:Young adult survivors of childhood cancer have an increased risk for treatment‐related morbidity and mortality. In this study, the authors assessed how treatment for childhood cancer affects older‐adult health and health practices.METHODS:One hundred seven adults treated for childhood cancer between 1947 and 1968, known to have survived past age 50 years, were identified from a single‐institution cohort established in 1975. Updated vital status on eligible cases was obtained from public records. Survivors and a control group of their age‐matched siblings and cousins completed a mailed survey to assess physical and social function, healthcare practices, and the prevalence of common adult illnesses.RESULTS:Of the 107 survivors known to be alive at age 50 years, 16 were deceased at follow‐up; 7 deaths could be associated with prior treatment (second malignancy in radiation field [3], small bowel obstruction after abdominal radiation [2], and cardiac disease after chest irradiation [2]). The 55 survivors (median age, 56 years; range, 51‐71 years), and 32 family controls (median age, 58 years; range, 48‐70 years), reported similar health practices, health‐related quality of life, and social function. However, survivors reported more frequent visits to healthcare providers (P < .05), more physical impairments (P < .05), fatigue (P = .02), hypertension (P = .001), and coronary artery disease (P = .01). An increased risk of hypertension was associated with nephrectomy during childhood (odds ratio, 18.9; 95% confidence interval, 3.0‐118.8).CONCLUSIONS:The oldest adult survivors of childhood cancer continue to be at risk for treatment‐related complications that potentially decrease their life expectancy and compromise their quality of life. Cancer 2010. © 2010 American Cancer Society.
Cytoplasmic mislocalization of the orphan nuclear receptor Nurr1 is a prognostic factor in bladder cancer
Authors: Inamoto Teruo , Czerniak Bogdan A. , Dinney Colin P. , Kamat Ashish M. 
Published: November 2009

AbstractBACKGROUND:Nurr1 belongs to a novel class of orphan nuclear receptors (the NR4A family). The authors have previously shown that Nurr1 is important in carcinogenesis. In the current study, they examined the clinicopathologic relevance of expression patterns of Nurr1 in bladder tumors.METHODS:Nurr1 expression was determined using immunohistochemical staining in a bladder cancer tissue array (145 tumors). Tumors were classified according to Nurr1 protein levels in both cytoplasm and nucleus. Disease‐specific survival and recurrence‐free survival were investigated by Kaplan‐Meier analysis and Cox proportional hazards analysis in multivariate models and correlated with variables such as tumor stage, growth pattern, and clinical outcome (recurrence and survival). In vitro, Nurr1 was examined for its role in bladder cancer cell proliferation and migration using small interfering RNA silencing.RESULTS:Nurr1 expression in tumor cells correlated with increasing tumor stage and invasive growth pattern. Disease‐specific survival was significantly shorter in patients whose tumors demonstrated a high level of cytoplasmic Nurr1 compared with those with lower levels of cytoplasmic Nurr1 expression. Furthermore, cytoplasmic Nurr1 expression level was found to be an independent predictor of disease‐specific survival (odds ratio, 4.894; P < .001). In vitro, silencing of endogenous Nurr1 attenuated the migration of bladder cancer cells.CONCLUSIONS:The expression of Nurr1 in the cytoplasm correlates with adverse outcome and is an independent prognostic marker for tumor progression and survival in patients with bladder cancer. This might represent a novel target in bladder cancer therapy. Cancer 2010. © 2010 American Cancer Society.
Development of monitoring system for FTTH‐PON using combined ACS and SANTAD
Authors: Ng Boonchuan , Ab‐Rahman Mohammad Syuhaimi , Premadi Aswir 
Published: November 2009

AbstractThis paper proposes a design of Access Control System (ACS) for fiber‐to‐the‐home passive optical network (FTTH‐PON). Our design works on a standard local area network using a specially designed hardware interfaced with a microcontroller integrated Ethernet to monitor the status of optical signals flow and provides the restoration against fiber failures/faults in FTTH‐PON. We also introduce the centralized troubleshooting system by means of Smart Access Network Testing, Analyzing, and Database (SANTAD). ACS is the subsystem that controls the troubleshooting mechanism carried out by SANTAD. This design will be implemented together with optical line terminal (OLT) at central office (CO) to centralized monitoring and for controlling each optical fiber line's status as well as detecting any failure that occurs in the network system downwardly from CO toward multiple optical network units at different customer residential locations. The scope of this discussion highlighted only the monitoring and controlling instead of the restoration scheme offered. Copyright © 2009 John Wiley & Sons, Ltd.
Photoluminescent Porous Modular Lanthanide–Vanadium–Organic Frameworks (Eur. J. Inorg. Chem. 33/2009)
Authors: Rocha João , Almeida Paz Filipe A. , Shi Fa‐Nian , Ferreira Rute A. S. , Trindade Tito , Carlos Luís D. 
Published: November 2009

AbstractThe cover picture shows the porous mixed‐metal anionic MOF isolated from the self‐assembly of cyclic trinuclear vanadium‐based anionic units with aqua‐based lanthanide complexes. The structure contains a three‐dimensional channel system filled with water molecules and charge‐balancing cations, in which the most prominent channels run parallel to the c axis. The material containing V4+ and Eu3+ permits the fine‐tuning of the photoluminescence emission from white to purple‐blue. Details are discussed in the article by J. Rocha, F. A. Almeida Paz et al. on p. 4931 ff.
Supramolecular Photochirogenesis with Carbenes Entrapped in Cyclodextrins (Eur. J. Org. Chem. 34/2009)
Authors: Mieusset Jean‐Luc , Wagner Gerald , Su Kuan‐Jen , Steurer Marianne , Pacar Mirjana , Abraham Michael , Brinker Udo H. 
Published: November 2009

AbstractThe cover picture shows the photochemical reaction of a diazirine encapsulated in the inherently chiral cavity of a cyclodextrin. The generated carbene affords intramolecular insertion products. For the first time for carbene reactions, products are imprinted, though modestly, with handedness derived from the cyclodextrins. Details are discussed in the article by U. H. Brinker et al. on page 5907 ff. The authors thank Mr. Michael Abraham for the photograph in the cover picture. The background shows the Vorderer Gosausee, Salzkammergut, Upper Austria. Large parts of the region were listed as a World Heritage Site in 1997.
Modeling interactions between leukemia‐specific chromosomal changes, somatic mutations, and gene expression patterns during progression of core‐binding factor leukemias
Authors: Jones Dan , Yao Hui , Romans Angela , Dando Caroline , Pierce Sherry , Borthakur Gautam , Hamilton Amy , Bueso‐Ramos Carlos , Ravandi Farhad , Garcia‐Manero Guillermo , Kantarjian Hagop 
Published: November 2009

AbstractIn cancer genomes, changes observed during tumor progression can be difficult to separate from nonspecific accumulation of cytogenetic changes due to cancer‐associated genetic instability. We studied genetic changes occurring over time in cancers presenting with a relatively simple karyotype, namely two related core‐binding factor (CBF) acute myeloid leukemias (AMLs), to assess how specific chromosomal changes are selected based on tumor subtype and acquired somatic mutations. Expression profiles for DNA replication/repair genes and the mutation status of KRAS, NRAS, FLT3, and KIT were compared with the karyotypic changes at diagnosis and relapse(s) in 94 cases of inv(16)(p13.1q22)‐AML and 82 cases of t(8;21)(q22;q22)‐AML. The majority of both AML types demonstrated a simple aneuploid pattern of cytogenetic progression, with highly distinctive patterns of chromosome copy number changes, such as +22 and +13 exclusively in inv(16)‐AML and −Y and −X in t(8;21)‐AML. Selection of certain cytogenetic changes correlated with particular somatic mutations, such as +8 with RAS mutation, and absence of kinase pathway mutations in t(8;21)‐AML with localized deletions at chromosome band 9q22. Alterations in transcript levels of mitotic spindle kinases such as CHEK1, AURKA, and AURKB were associated with the aneuploid progression pattern, particularly in t(8;21) cases. Despite the similarity in the initiating genetics of the two CBF AML types, highly tumor‐specific patterns of limited aneuploidy are noted that persist and continue to accumulate at relapse. Thus, activation of genetic instability, possibly through mitotic spindle dysregulation, leads rapidly to the selection of advantageous single chromosome aneuploidy. © 2009 Wiley‐Liss, Inc.
Glial cells promote dendrite formation and the reception of synaptic input in Purkinje cells from postnatal mice
Authors: Buard Isabelle , Steinmetz Celine C. , Claudepierre Thomas , Pfrieger Frank W. 
Published: November 2009

AbstractPrevious studies suggest that glial cells contribute to synaptogenesis in specific neurons from the postnatal CNS. Here, we studied whether this is true for Purkinje cells (PCs), which represent a unique neuronal cell type due to their large size, massive synaptic input, and high vulnerability. Using new glia‐free cultures enriched in PCs from postnatal mice we show that these neurons survived and grew, but displayed only low levels of excitatory and inhibitory synaptic activity. Coculture with glial cells strongly enhanced the frequency and size of spontaneous and miniature excitatory synaptic currents as well as neurite growth and branching. Immunocytochemical staining for microtubule‐associated protein 2‐ (MAP2‐) positive neurites revealed impaired dendrite formation in PCs under glia‐free conditions, which can explain the absence of synaptic activity. Glial signals strongly enhanced dendritogenesis in PCs and thus their ability to receive excitatory synaptic input from granule cells (GCs). The enhancement of dendrite formation was mimicked by glia‐conditioned medium (GCM), whereas the increase in synaptic activity required physical presence of glia. This indicated that dendrite development is necessary but not sufficient for PCs to receive excitatory synaptic input and that synaptogenesis requires additional signals. The level of inhibitory synaptic activity was low even in cocultures due to a low incidence of inhibitory interneurons. Taken together, our results reinforce the idea that glial cells promote synaptogenesis in specific neuronal cell types. © 2009 Wiley‐Liss, Inc.