bluuebunny/crossref_metadata_2025_split huggingface dataset:
Progress of Research in Cell‐in‐Cell Phenomena
Authors: Yang Yue‐Qin , Li Ji‐Cheng 
Published: January 2012

AbstractThe discovery of a nonphagocytotic process of cell‐in‐cell phenomena can be traced to over a century ago. However, its biological significance remains poorly understood. Three types of cell‐in‐cell phenomena have been described so far, termed “cannibalism,” “emperipolesis,” and “entosis.” These three kinds of cell‐in‐cell phenomena, apart from a common feature of one cell internal to another, are distinct both cytologically and biologically. In this review, we discussed them in their morphology, cell recognition, penetration mechanisms, and physiological roles, respectively. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc.
siRNA high‐throughput kinase library screen identifies protein kinase, DNA‐activated catalytic polypeptide to play a role in MyD88‐induced IFNA2 activation and IL‐8 secretion
Authors: Charlaftis Nikolaos , Fearon Douglas T. , Schoenemeyer Annett , Morley Peter J. 
Published: January 2012

AbstractThe discovery of RNA interference has led to the development of short interfering RNA (siRNA) screening, which has been widely used to study biological pathways. Here, we describe the development and validation of a system suitable to identify cellular genes involved in interferon A2 (IFNA2) promoter activation and interleukin (IL)‐8 secretion downstream of MyD88. Forty genes were identified. Five genes were selected for further study. One gene, protein kinase, DNA‐activated catalytic polypeptide (PRKDC), was confirmed to play a role in MyD88‐induced IFNA2 activation and IL‐8 secretion.
Effect of the timing of food intake on the absorption and bioavailability of carbamazepine immediate‐release tablets in beagle dogs
Authors: Xu Cai‐hong , Cheng Gang , Liu Yi , Tian Ye , Yan Jing , Zou Mei‐juan 
Published: January 2012

ABSTRACTThe effect of dosing time on the bioavailability of carbamazepine immediate‐release (IR) tablets was investigated when administered to beagle dogs who were fasting, with co‐administration of food (Co‐food), and 0.5 h before food and 2 h after food. The study was conducted using a single dose of 200 mg (tablets/solution) with a 2 week washout period in a crossover design. Food intake significantly increased the rate and extent of tablet absorption. The Cmax (µg·ml−1, 8.13/3.65) and tmax (h, 1.83/0.92) were increased more than two‐fold and the AUC0‐24 (µg·h·ml−1, 20.09/8.19) was 2.5 times that of the values obtained under fasting conditions. The bioavailability of the tablets under fasting conditions was 91.2%, but increased to 223.5%, 182.8% and 148.4% in the Co‐food, 0.5 h before food and 2 h after food groups, respectively (p < 0.05). Although there was no significant difference in the Cmax or AUC0‐24 between the treatments with food, the absorption appeared to be reduced to some extent when the tablets were given 2 h after food. The oral bioavailability of CBZ IR tablets was significantly affected by the timing of the food intake. This is maybe favored by the fluctuations in the level of bile salts with the timing of food intake. To obtain acute therapy for a drug with narrow therapeutic window, attention should be given to the dosing time and food intake interactions. Copyright © 2012 John Wiley & Sons, Ltd.
In situ LTE exposure of the general public: Characterization and extrapolation
Authors: Joseph Wout , Verloock Leen , Goeminne Francis , Vermeeren Günter , Martens Luc 
Published: January 2012

AbstractIn situ radiofrequency (RF) exposure of the different RF sources is characterized in Reading, United Kingdom, and an extrapolation method to estimate worst‐case long‐term evolution (LTE) exposure is proposed. All electric field levels satisfy the International Commission on Non‐Ionizing Radiation Protection (ICNIRP) reference levels with a maximal total electric field value of 4.5 V/m. The total values are dominated by frequency modulation (FM). Exposure levels for LTE of 0.2 V/m on average and 0.5 V/m maximally are obtained. Contributions of LTE to the total exposure are limited to 0.4% on average. Exposure ratios from 0.8% (LTE) to 12.5% (FM) are obtained. An extrapolation method is proposed and validated to assess the worst‐case LTE exposure. For this method, the reference signal (RS) and secondary synchronization signal (S‐SYNC) are measured and extrapolated to the worst‐case value using an extrapolation factor. The influence of the traffic load and output power of the base station on in situ RS and S‐SYNC signals are lower than 1 dB for all power and traffic load settings, showing that these signals can be used for the extrapolation method. The maximal extrapolated field value for LTE exposure equals 1.9 V/m, which is 32 times below the ICNIRP reference levels for electric fields. Bioelectromagnetics 33:466–475, 2012. © 2012 Wiley Periodicals, Inc.
Excitable behavior can explain the “ping‐pong” mode of communication between cells using the same chemoattractant
Authors: Goryachev Andrew B. , Lichius Alexander , Wright Graham D. , Read Nick D. 
Published: January 2012

AbstractHere we elucidate a paradox: how a single chemoattractant‐receptor system in two individuals is used for communication despite the seeming inevitability of self‐excitation. In the filamentous fungus Neurospora crassa, genetically identical cells that produce the same chemoattractant fuse via the homing of individual cell protrusions toward each other. This is achieved via a recently described “ping‐pong” pulsatile communication. Using a generic activator‐inhibitor model of excitable behavior, we demonstrate that the pulse exchange can be fully understood in terms of two excitable systems locked into a stable oscillatory pattern of mutual excitation. The most puzzling properties of this communication are the sudden onset of oscillations with final amplitude, and the absence of seemingly inevitable self‐excitation. We show that these properties result directly from both the excitability threshold and refractory period characteristic of excitable systems. Our model suggests possible molecular mechanisms for the ping‐pong communication.
Physical and chemical aspects of stabilization of compounds in silk
Authors: Pritchard Eleanor M. , Dennis Patrick B. , Omenetto Fiorenzo , Naik Rajesh R. , Kaplan David L. 
Published: January 2012

AbstractThe challenge of stabilization of small molecules and proteins has received considerable interest. The biological activity of small molecules can be lost as a consequence of chemical modifications, while protein activity may be lost due to chemical or structural degradation, such as a change in macromolecular conformation or aggregation. In these cases, stabilization is required to preserve therapeutic and bioactivity efficacy and safety. In addition to use in therapeutic applications, strategies to stabilize small molecules and proteins also have applications in industrial processes, diagnostics, and consumer products like food and cosmetics. Traditionally, therapeutic drug formulation efforts have focused on maintaining stability during product preparation and storage. However, with growing interest in the fields of encapsulation, tissue engineering, and controlled release drug delivery systems, new stabilization challenges are being addressed; the compounds or protein of interest must be stabilized during: (1) fabrication of the protein or small molecule‐loaded carrier, (2) device storage, and (3) for the duration of intended release needs in vitro or in vivo. We review common mechanisms of compound degradation for small molecules and proteins during biomaterial preparation (including tissue engineering scaffolds and drug delivery systems), storage, and in vivo implantation. We also review the physical and chemical aspects of polymer‐based stabilization approaches, with a particular focus on the stabilizing properties of silk fibroin biomaterials. © 2011 Wiley Periodicals, Inc. Biopolymers 97: 479–498, 2012.
Correlation of CT aortography measurements of infrarenal aortic aneurysms and body mass index in preprocedural evaluation for endovascular repair
Authors: Mladenovic Ana S. , Markovic Zeljko Z. , Hyodoh Hideki H. , Stosic‐Opincal Tatjana 
Published: January 2012

AbstractThe aim of this study is to analyze the morphological differences of infrarenal aortic aneurysms and common iliac arteries that are important for endovascular management between patients of different body mass index using 64 slice multidetector row CT aortography. This was a multicenter study of 100 patients (50 Europeans and 50 Japanese). All patients had risk factors, manifest symptoms, and ultrasound verified aneurysmal dilation of the infrarenal aorta. All examinations were performed on the same CT platform using the same post‐processing protocols. Due to the heterogeneity of the population, several statistical models were used. Significant differences were found in morphological parameters of infrarenal aorta in relation to BMI. In over one out of three patients with BMI less than 23, endovascular treatment is contraindicated due to the dimensions of the aneurysmal neck. Relative to BMI value, differences were found in transverse diameters of the medium part of the aneurysm and in the length of common iliac arteries. CT aortography performed on a 64 slice multidetector row CT platform provides precise and numerous data for the analysis of anatomical and pathological differences of infrarenal aortic aneurysms that are of crucial importance for the planning of treatment and the analysis of the differences relating to body habitus. Clin. Anat. 25:767–772, 2012. © 2012 Wiley‐Liss, Inc.
Base Pairing at the Abasic Site in DNA Duplexes and Its Application in Adenosine Aptasensors
Authors: Pang Yuanfeng , Xu Zhiai , Sato Yusuke , Nishizawa Seiichi , Teramae Norio 
Published: January 2012

AbstractThe binding of nucleosides to abasic site (AP site)‐containing DNA duplexes (AP‐DNAs) carrying complementary nucleosides opposite the AP site was investigated by thermal denaturation and isothermal titration calorimetric (ITC) experiments. Purine nucleosides show high affinities (Kd=14.1 μM for adenosine and 41.8 μM for guanosine) for binding to the AP‐DNAs, and the interactions are driven primarily by the enthalpy change, similarly to the case of DNA intercalators. In contrast, pyrimidine nucleosides do not show noticeable binding to the AP‐DNAs, thus suggesting that stacking interaction at the AP site plays a key role in the binding of purine nucleosides to the AP‐DNAs, as revealed by ITC measurements. Next, to apply an AP‐DNA as an aptasensor for adenosine, a competitive assay between adenosine and AP‐site‐binding fluorescent ligand was performed. The assay employs a fluorescent ligand, riboflavin, that binds to the AP site in a DNA duplex, thereby causing fluorescence quenching. By adding adenosine to the riboflavin/AP‐DNA complex, the binding of adenosine to the AP site causes release of riboflavin from the AP site, thereby resulting in restoration of riboflavin fluorescence. AP‐DNAs can serve as a new class of aptasensors—a limit of detection of 0.7 μM was obtained for adenosine. In contrast to conventional aptasensors for adenosine, the present method shows high selectivity for adenosine over the other nucleotides (AMP, ADP and ATP). The method does not require covalent labelling of fluorophores, and thus it is cost‐effective; finally, the method was successfully demonstrated to be applicable for the detection of adenosine in horse serum.
An Adaptable Luminescence Resonance Energy Transfer Assay for Measuring and Screening Protein–Protein Interactions and their Inhibition.
Authors: Yapici Engin , Reddy D. Rajasekhar , Miller Lawrence W. 
Published: January 2012

AbstractProtein–protein interactions (PPIs) are central to biological processes and represent an important class of therapeutic targets. Here we show that the interaction between FK506‐binding protein 12 fused to green fluorescent protein (GFP‐FKBP) and the rapamycin‐binding domain of mTor fused to Escherichia coli dihydrofolate reductase (FRB‐eDHFR) can be sensitively detected (signal‐to‐background ratio (S/B)>100) and accurately quantified within an impure cell lysate matrix using a luminescence resonance energy transfer (LRET) assay. Ascomycin‐mediated inhibition of GFP‐FKBP–rapamycin–FRB‐eDHFR complex formation was also detected at high S/B ratio (>80) and Z′‐factor (0.89). The method leverages the selective, stable binding of trimethoprim (TMP)‐terbium complex conjugates to eDHFR, and time‐resolved, background‐free detection of the long‐lifetime (∼ms) terbium‐to‐GFP LRET signal that indicates target binding. TMP–eDHFR labeling can be adapted to develop high‐throughput screening assays and complementary, quantitative counter‐screens for a wide variety of PPI targets with a broad range of affinities that may not be amenable to purification.
Porous YAG:Nd3+ Fibers with Excitation and Emission in the Human “NIR Optical Window” as Luminescent Drug Carriers
Authors: Ma Zhijun , Ji Huijiao , Tan Dezhi , Dong Guoping , Teng Yu , Zhou Jiajia , Qiu Jianrong 
Published: January 2012

AbstractThe design and preparation of luminescent drug carriers has been a prosperous area of research for many years. However, the excitation and/or emission wavelength of such luminescent drug carriers haven′t been optimized in the so‐called human “near infrared (NIR) optical window”, thus restricting their practical applications. Herein, we report the synthesis of electrospun porous YAG:Nd3+ (neodymium‐doped yttrium aluminum garnet) fibers with both excitation and emission in the “NIR optical window” as luminescent drug carriers. The YAG:Nd3+ porous fibers were characterized by SEM, TEM, XRD, scanning transmission electron microscopy–energy‐dispersive X‐ray spectroscopy (STEM‐EDX), and photoluminescence (PL). Ibuprofen (IBU) was used as a model drug to evaluate the drug‐loading capacities and release profiles of the samples. BMSCs (bone mesenchymal stem cells) were used as model human cells to investigate cytotoxicity. Our results indicated that the YAG:Nd3+ fibers possessed a fine, irregularly porous fibrous morphology with an average diameter of 378 nm. The florescence of the sample (1064 nm) could be excited over a wide wavelength range in the NIR region. During the release process of IBU in simulated body fluid (SBF), along with the dissolving of the drug, the solvent entered into the pores, and the emission intensity of the YAG:Nd3+ fibers at 1064 nm decreased gradually, owing to a quenching effect of the hydroxy groups, thus provided an approach to track and monitor drug release. In addition, cytotoxicity investigations revealed that these YAG:Nd3+ fibers were biocompatible with human cells. Consequently, the porous YAG:Nd3+ fibers are a promising material for applications as advanced drug carriers.
ChemInform Abstract: Synthesis, Crystal Structure and Chemical Bonding of the Zintl Phase Rb7NaSi8.
Authors: Goebel Thorsten , Prots Yurii , Ormeci Alim , Pecher Oliver , Haarmann Frank 
Published: January 2012

AbstractThe title phase is synthesized from stoichiometric amounts of Na4Si4 and Rb4Si4 (500 °C, 7 d) and characterized by single crystal and powder XRD, MAS NMR spectroscopy, magnetic measurements, and quantum chemical calculations (all electron, full‐potential local orbital minimum basis method).
ChemInform Abstract: A New Emission Band of Eu2+ and Its Efficient Energy Transfer to Mn2+ in Sr2Mg3P4O15:Mn2+, Eu2+.
Authors: Yu Jinying , Hao Zhendong , Zhang Xia , Luo Yongshi , Zhang Jiahua 
Published: January 2012

AbstractEu2+‐doped and Eu2+/Mn2+ co‐doped Sr2Mg3P4O15 exhibit a new emission band centered at 399 nm in addition to the well known band at 448 nm.
ChemInform Abstract: [(Ph)3PBr][Br7], [(Bz)(Ph)3P]2[Br8], [(n‐Bu)3MeN]2 [Br20], [C4MPyr]2[Br20], and [(Ph)3PCl]2[Cl2I14]: Extending the Horizon of Polyhalides via Synthesis in Ionic Liquids.
Authors: Wolff Michael , Okrut Alexander , Feldmann Claus 
Published: January 2012

AbstractThe polyhalides (II), (III), and (V) are prepared from dibromine in eutectic, equimolar mixtures of the ionic liquids (dpyr)Br and (bpyr)CF3SO3.
ChemInform Abstract: Methanesulfonates of High‐Valent Metals: Syntheses and Structural Features of MoO2(CH3SO3)2, UO2(CH3SO3)2, ReO3(CH3SO3), VO(CH3SO3)2, and V2O3(CH3SO3)4 and Their Thermal Decomposition under N2 and O2 Atmosphere.
Authors: Betke Ulf , Neuschulz Kai , Wickleder Mathias S. 
Published: January 2012

AbstractThe title compounds are the first examples of solvent‐free oxide methanesulfonates of Mo, U, Re, and V.
ChemInform Abstract: Isolation of a Stable Lacunary Dawson‐Type Polyoxomolybdate Cluster.
Authors: Hashikawa Akihiro , Fujimoto Makiko , Hayashi Yoshihito , Miyasaka Hitoshi 
Published: January 2012

AbstractThe hexa‐lacunary Dawson type polyoxomolybdate (Bu4N)2 [MoV4MoVI10O38 (OAc)6] (I) is synthesized from (Bu4N)2[Mo6O19] dissolved in MeCN and a mixture of acetic acid and acetic anhydrid solution in the presence of Bu3SnH (N2, reflux, 12 h, 45% yield).
ChemInform Abstract: Metal‐Centered Deltahedral Zintl Ions: Synthesis of [Ni@Sn9]4‐ by Direct Extraction from Intermetallic Precursors and of the Vertex‐Fused Dimer [{Ni@Sn8(μ‐Ge)1/2}2]4‐.
Authors: Gillett‐Kunnath Miriam M. , Paik Joseph I. , Jensen Sara M. , Taylor Jacob D. , Sevov Slavi C. 
Published: January 2012

AbstractThe endohedral cluster [Ni@Sn9]4‐ is extracted in en from the new precursors (I), (IV), and (VII), which are obtained from mixtures of the elements (Nb containers, 950 °C, 2 d).
ChemInform Abstract: Synthesis, Structure and Chemical Bonding of Ba2Ni5Ge4 — An Intermetallic Compound with a New Two‐Dimensional ∞2[Ni5Ge4] Structural Motif.
Authors: Siggelkow Lisa , Hlukhyy Viktor , Faessler Thomas F. 
Published: January 2012

AbstractThe title compound is synthesized by arc melting of stoichiometric mixtures of the elements followed by annealing at 800 °C for two weeks under argon.
ChemInform Abstract: Protective Opening of Epoxide Using Pivaloyl Halides under Catalyst‐Free Conditions.
Authors: Rao Chitturi Bhujanga , Rao Dasireddi Chandra , Venkateswara Mallem , Venkateswarlu Yenamandra 
Published: January 2012

AbstractAn efficient, mild, and environmentally benign protocol for the regioselective opening of epoxides is reported.
ChemInform Abstract: N‐Heterocyclic Carbene‐Catalyzed Aza‐Michael Addition.
Authors: Kang Qiang , Zhang Yugen 
Published: January 2012

AbstractA novel aza‐Michael addition of amines, including aromatic and aliphatic amines, with α,β‐unsaturated ketones is realized by employing N‐heterocyclic carbene as bifunctional catalyst.
ChemInform Abstract: Hydrosilanes Are not Always a Reducing Reagent: A Ruthenium‐Catalyzed Introduction of Primary Alkyl Groups to Electron‐Rich Aromatic Rings Using Esters as a Source of the Alkyl Groups.
Authors: Nagashima Hideo , Kubo Yuichi , Kawamura Mitsunobu , Nishikata Takashi , Motoyama Yukihiro 
Published: January 2012

AbstractAlthough it is limited to electron‐rich aromatic compounds, the combination of a hydrosilane, an ester, and the ruthenium cluster provides a good method to introduce a primary alkyl group to the aromatic compound.
ChemInform Abstract: Synthesis of Fused Carbocycles via a Selective 6‐Endo Dig Gold(I)‐Catalyzed Carbocyclization.
Authors: Barabe Francis , Levesque Patrick , Korobkov Ilia , Barriault Louis 
Published: January 2012

AbstractA broad spectrum of substrates is used for the transformations in the presence of gold catalysts (I)‐(IV).
ChemInform Abstract: Synthesis of Cyclopentenols and Cyclopentenones via Nickel‐Catalyzed Reductive Cycloaddition.
Authors: Jenkins Aireal D. , Herath Ananda , Song Minsoo , Montgomery John 
Published: January 2012

AbstractWhile the “normal” cycloaddition between dienes and alkynes leads to formation of six‐membered Diels—Alder or hetero‐Diels—Alder ring products, the reductive cycloaddition using BEt3 as the active reducing agent and MeOH as the proton donor allows the synthesis of either cyclopentenols (from enals and alkynes) or cyclopentenones (from enoates and alkynes).
ChemInform Abstract: Bifunctional Phosphine‐Catalyzed Cross‐Rauhut—Currier/Michael/Aldol Condensation Triple Domino Reaction: Synthesis of Functionalized Cyclohexenes.
Authors: Xie Peizhong , Huang You , Lai Wenqing , Meng Xiangtai , Chen Ruyu 
Published: January 2012

AbstractThis three‐component domino reaction offers a powerful approach to highly substituted cyclohexene skeletons.
ChemInform Abstract: Facile and Chemoselective Rhodium‐Catalyzed Intramolecular Hydroacylation of α,α‐Disubstituted 4‐Alkylidenecyclopropanals.
Authors: Crepin Damien , Tugny Coralie , Murray James H. , Aissa Christophe 
Published: January 2012

AbstractMild intramolecular hydroacylation of α,α‐disubstituted 4‐alkylidenecyclopropanals, such (I), (VII), and (IX) is described avoiding decarbonylation and affording cycloheptenones (II), (VIII), and (X) in good yields.
ChemInform Abstract: Oxidation of Primary Aliphatic and Aromatic Aldehydes with Difluoro(aryl)‐λ3‐Bromane.
Authors: Ochiai Masahito , Yoshimura Akira , Hoque Md. Mahbubul , Okubo Takuji , Saito Motomichi , Miyamoto Kazunori 
Published: January 2012

AbstractAcid fluorides are produced from primary aliphatic aldehydes, whereas the aromatic substrates form difluoromethyl ethers due to a larger migratory aptitude of the aryl groups.
ChemInform Abstract: Highly Diastereo‐ and Enantioselective Tandem Reaction Toward Functionalized Pyrrolidines with Multiple Stereocenters.
Authors: Guo Shengmei , Xie Yinjun , Hu Xinquan , Huang Hanmin 
Published: January 2012

AbstractA broad spectrum of dual Michael adducts is prepared with excellent diastereoselectivity (<90% d.e.).
ChemInform Abstract: Turning a Riboflavin‐Binding Protein into a Self‐Sufficient Monooxygenase by Cofactor Redesign.
Authors: de Gonzalo Gonzalo , Smit Christian , Jin Jiangfeng , Minnaard Adriaan J. , Fraaije Marco W. 
Published: January 2012

AbstractTight binding of N‐alkylated flavins (I) to riboflavin‐binding protein results in the creation of artifical flavoenzymes capable of H2O2 mediated enantioselective sulfoxidation.
ChemInform Abstract: Solid‐State Suzuki Reaction Catalyzed by Ferrocene Palladacycles Without Solvent.
Authors: Khruscheva N. S. , Bulygina L. A. , Sokolov V. I. 
Published: January 2012

AbstractA solventless Suzuki reaction using ferrocene palladacycles with bi‐ and tridentate (C,N) and (C,N,N) ligands is achieved.
ChemInform Abstract: N‐Heterocyclic Carbene/Pd(II)/1‐Methylimidazole Complex Catalyzed Suzuki—Miyaura Coupling Reaction of Aryl Chlorides in Water.
Authors: Zhou Xia‐Xia , Shao Li‐Xiong 
Published: January 2012

AbstractThe well‐defined complex shows high activity in the title process.
ChemInform Abstract: Stereoselective Thio‐Michael Addition to Chalcones in Water Catalyzed by Bovine Serum Albumin.
Authors: Gaggero Nicoletta , Albanese Domenico Carlo Maria , Celentano Giuseppe , Banfi Stefano , Aresi Alice 
Published: January 2012

AbstractThe title non‐enzymic protein catalyzes the addition of aromatic or aliphatic thiols to α,β‐unsaturated ketones in a stereoselective fashion to give enantioenriched β‐sulfanyl ketones.
ChemInform Abstract: Double Arylation of Allyl Alcohol via a One‐Pot Heck Arylation—Isomerization—Acylation Cascade.
Authors: Colbon Paul , Ruan Jiwu , Purdie Mark , Molholland Keith , Xiao Jianliang 
Published: January 2012

AbstractThe arylation step favors aryl bromides bearing electron‐withdrawing substituents, while the acylation reaction is supported by electron‐donating groups.
ChemInform Abstract: Synthesis and Structure of Hypervalent Diacetoxybromobenzene and Aziridination of Olefins with Imino‐λ3‐bromane Generated in situ under Metal‐Free Conditions.
Authors: Hoque Md. Mahbubul , Miyamoto Kazunori , Tada Norihiro , Shiro Motoo , Ochiai Masahito 
Published: January 2012

AbstractIn most cases, the reaction is carried out by first premixing bromane (III) and triflylamide (IV) followed by addition of the olefin.