bluuebunny/crossref_metadata_2025_split huggingface dataset:
Smaller stencil preconditioners for linear systems in RBF-FD discretizations
Authors: Koch Michael , Le Borne Sabine , Leinen Willi 
Published: April 2024

Abstract
Radial basis function finite difference (RBF-FD) discretization has recently emerged as an alternative to classical finite difference or finite element discretization of (systems) of partial differential equations. In this paper, we focus on the construction of preconditioners for the iterative solution of the resulting linear systems of equations. In RBF-FD, a higher discretization accuracy may be obtained by increasing the stencil size. This, however, leads to a less sparse and often also worse conditioned stiffness matrix which are both challenges for subsequent iterative solvers. We propose to construct preconditioners based on stiffness matrices resulting from RBF-FD discretization with smaller stencil sizes compared to the one for the actual system to be solved. In our numerical results, we focus on RBF-FD discretizations based on polyharmonic splines (PHS) with polynomial augmentation. We illustrate the performance of smaller stencil preconditioners in the solution of the three-dimensional convection-diffusion equation.
Situated Affects and Place Memory
Authors: Sutton John 
Published: April 2024

AbstractTraces of many past events are often layered or superposed, in brain, body, and world alike. This often poses challenges for individuals and groups, both in accessing specific past events and in regulating or managing coexisting emotions or attitudes. We sometimes struggle, for example, to find appropriate modes of engagement with places with complex and difficult pasts. More generally, there can appear to be a tension between what we know about the highly constructive nature of remembering, whether it is drawing on neural or worldly resources or both, and the ways that we need and use memory to make claims on the past, and to maintain some appropriate causal connections to past events. I assess the current state of work on situated affect and distributed memory, and the recent criticisms of the ‘dogma of harmony’ in these fields. I then deploy these frameworks to examine some affective dimensions of place memory, sketching a strongly distributed conception of places as sometimes partly constituting the processes and activities of feeling and remembering. These approaches also offer useful perspectives on the problems of how to engage – politically and aesthetically – with difficult pasts and historically burdened heritage. In assessing artistic interventions in troubled places, we can seek responsibly to do justice to the past while fully embracing the dynamic and contested constructedness of our present emotions, memories, and activities.
Bacterial extracellular vesicles: biotechnological perspective for enhanced productivity
Authors: Muñoz-Echeverri Laura M. , Benavides-López Santiago , Geiger Otto , Trujillo-Roldán Mauricio A. , Valdez-Cruz Norma A. 
Published: April 2024

AbstractBacterial extracellular vesicles (BEVs) are non-replicative nanostructures released by Gram-negative and Gram-positive bacteria as a survival mechanism and inter- and intraspecific communication mechanism. Due to BEVs physical, biochemical, and biofunctional characteristics, there is interest in producing and using them in developing new therapeutics, vaccines, or delivery systems. However, BEV release is typically low, limiting their application. Here, we provide a biotechnological perspective to enhance BEV production, highlighting current strategies. The strategies include the production of hypervesiculating strains through gene modification, bacteria culture under stress conditions, and artificial vesicles production. We discussed the effect of these production strategies on BEVs types, morphology, composition, and activity. Furthermore, we summarized general aspects of BEV biogenesis, functional capabilities, and applications, framing their current importance and the need to produce them in abundance. This review will expand the knowledge about the range of strategies associated with BEV bioprocesses to increase their productivity and extend their application possibilities.
Graphical abstract
P2X7 receptor: a potential target for treating comorbid anxiety and depression
Authors: Liu Jun , Liu Ting-Ting , Mou Lan , Zhang Yuwen , Chen Xiang , Wang Qi , Deng Bin-Lu , Liu Jie 
Published: April 2024

AbstractIn clinical practice, depression and anxiety frequently coexist, and they are both comorbid with somatic diseases. The P2X7R is an adenosine 5’-triphosphate (ATP)-gated non-selective cation channel that is widely expressed in immune-related cells. Under conditions of stress, chronic pain, and comorbid chronic physical illness, P2X7R activation in glial cells leads to neuroinflammation. This could contribute to the development of anxiety and depression-related emotional disturbances. Previous studies have shown that the P2X7 receptor (P2X7R) plays an important role in the pathogenesis of both anxiety and depression. Thus, the P2X7R may play a role in the comorbidity of anxiety and depression. Positron emission tomography can be used to assess the degree and location of neuroinflammation by monitoring functional and expression-related changes in P2X7R, which can facilitate clinical diagnoses and guide the treatment of patients with anxiety and depression. Moreover, single nucleotide polymorphisms (SNPs) in the P2X7R gene are associated with susceptibility to different types of psychiatric disorders. Thus, evaluating the SNPs of the P2X7R gene could enable personalized mood disorder diagnoses and treatments. If the P2X7R were set as a therapeutic target, selective P2X7R antagonists may modulate P2X7R function, thereby altering the balance of intra- and extra-cellular ATP. This could have therapeutic implications for treating anxiety and depression, as well as for pain management. According to in vitro and in vivo studies, the P2X7R plays an important role in anxiety and depression. In this review, we consider the potential of the P2X7R as a therapeutic target for comorbid anxiety and depression, and discuss the potential diagnostic and therapeutic value of this receptor.
How does a fly die? Insights into ageing from the pathophysiology of Drosophila mortality
Authors: dos Santos Eliano , Cochemé Helena M. 
Published: April 2024

AbstractThe fruit fly Drosophila melanogaster is a common animal model in ageing research. Large populations of flies are used to study the impact of genetic, nutritional and pharmacological interventions on survival. However, the processes through which flies die and their relative prevalence in Drosophila populations are still comparatively unknown. Understanding the causes of death in an animal model is essential to dissect the lifespan-extending interventions that are organism- or disease-specific from those broadly applicable to ageing. Here, we review the pathophysiological processes that can lead to fly death and discuss their relation to ageing.
Factors Influencing Educators’ Curiosity to Learn about VR Technologies in Education
Authors: Doz Daniel , Krajnčan Mitja , Jenko Matija , Vukovič Matej 
Published: April 2024

AbstractWhile recent research has extensively explored the potential use of Virtual Reality (VR) in educational contexts, limited knowledge exists regarding teachers’ willingness to further their understanding of VR and its applications in education. Therefore, this study aims to investigate how various demographic factors and attitudes toward VR influence educators’ curiosity about this technology. Using the boosting classification machine-learning algorithm, we analyzed the responses of 324 educators from Europe and Eurasia. The findings reveal that the regularity of undergoing pedagogical training exerts the strongest influence on educators’ interest in learning about VR technology, underscoring the importance of consistent training opportunities. Additionally, frequent personal testing and use of VR technology, along with the duration of professional education in pedagogy, significantly affect educators’ curiosity. Age and gender identity also play influential roles, with older educators and males exhibiting greater curiosity. Notably, educators’ awareness of VR technology has a relatively lower impact. Furthermore, the total years of teaching experience and prior use of VR technology in teaching have minimal effects on educators’ curiosity. These findings emphasize the significance of providing high-quality pedagogical training to familiarize educators with VR technology and its potential benefits in educational settings, carrying substantial implications for policymakers.
Untangling the Molecular Interactions Underlying Intracellular Phase Separation Using Combined Global Sensitivity Analyses
Authors: Gasior Kelsey I. , Cogan Nicholas G. 
Published: April 2024

AbstractLiquid-liquid phase separation is an intracellular mechanism by which molecules, usually proteins and RNAs, interact and then rapidly demix from the surrounding matrix to form membrane-less compartments necessary for cellular function. Occurring in both the cytoplasm and the nucleus, properties of the resulting droplets depend on a variety of characteristics specific to the molecules involved, such as valency, density, and diffusion within the crowded environment. Capturing these complexities in a biologically relevant model is difficult. To understand the nuanced dynamics between proteins and RNAs as they interact and form droplets, as well as the impact of these interactions on the resulting droplet properties, we turn to sensitivity analysis. In this work, we examine a previously published mathematical model of two RNA species competing for the same protein-binding partner. We use the combined analyses of Morris Method and Sobol’ sensitivity analysis to understand the impact of nine molecular parameters, subjected to three different initial conditions, on two observable LLPS outputs: the time of phase separation and the composition of the droplet field. Morris Method is a screening method capable of highlighting the most important parameters impacting a given output, while the variance-based Sobol’ analysis can quantify both the importance of a given parameter, as well as the other model parameters it interacts with, to produce the observed phenomena. Combining these two techniques allows Morris Method to identify the most important dynamics and circumvent the large computational expense associated with Sobol’, which then provides more nuanced information about parameter relationships. Together, the results of these combined methodologies highlight the complicated protein-RNA relationships underlying both the time of phase separation and the composition of the droplet field. Sobol’ sensitivity analysis reveals that observed spatial and temporal dynamics are due, at least in part, to high-level interactions between multiple (3+) parameters. Ultimately, this work discourages using a single measurement to extrapolate the value of any single rate or parameter value, while simultaneously establishing a framework in which to analyze and assess the impact of these small-scale molecular interactions on large-scale droplet properties.
The association of FRAX with predictors of falls in the assessment of postmenopausal osteoporosis in Turkey: the fracture study of Turkey (FRACT study)
Authors: Tuzun Sansin , Akarirmak Ulku , Kulaksiz Bilal , Keles Aslinur , Okutan Dilara , Karsli Pinar , Kurtoglu Sevgi Selin , Aygun Eren , Palamar Deniz 
Published: April 2024

Abstract
Summary
Although FRAX is used for fracture risk evaluation, this tool does not include balance and fall risk. The association between the predictors of falls and high FRAX scores we found in this study suggests that risk indicators for falls may add substantial value to FRAX by improving fracture risk prediction.

Purpose
This observational, descriptive, and cross-sectional study aimed to assess the fall risk predictors and explore their association with FRAX in Turkish patients with postmenopausal osteoporosis.

Methods
Two hundred and nine (209) women with postmenopausal osteoporosis referred to the Fracture Liaison Service (FLS) at Istanbul University–Cerrahpaşa were enrolled in the FRACT study (The Fracture Study of Turkey). Clinical risk factors were assessed using the FRAX tool. Tandem stance, Tandem walking, Timed up and go (TUG), and Chair stand tests were performed to assess balance and fall risk.

Results
Among patients with a mean age of 67.6 (± 9.7) years, 66 patients (31.6%) had osteoporosis without fractures and 143 patients (68.4%) had fragility fractures. The proportion of patients with poor performance of fall prediction tests was significantly higher in patients with a fragility fracture than those with osteoporosis alone. There was an inverse relationship between dynamic balance tests and the reported number of prior falls in the past year. FRAX score was higher in patients with impaired Tandem stance, Tandem walking, and TUG tests (p = 0.008, p = 0.035, p = 0.001, respectively).

Conclusion
Assessment of fall risk predictors should be one of the major pillars in the physical evaluation of osteoporotic patients in the FLS setting. FRAX is a useful tool to determine the fracture risk of patients with both static and dynamic balance impairments. Combining balance assessment with FRAX may be an important step to optimize osteoporosis risk assessment.
Selective vulnerability of hippocampal sub-regions in patients with subcortical vascular mild cognitive impairment
Authors: Chen Jianxiang , Wang Jianjun , Duan Ke , Li Xinbei , Pan Zhongxian , Zhang Jinhuan , Qin Xiude , Hu Yuanming , Lyu Hanqing 
Published: April 2024

AbstractEarly diagnosis of subcortical vascular mild cognitive impairment (svMCI) is clinically essential because it is the most reversible subtype of all cognitive impairments. Since structural alterations of hippocampal sub-regions have been well studied in neurodegenerative diseases with pathophysiological cognitive impairments, we were eager to determine whether there is a selective vulnerability of hippocampal sub-fields in patients with svMCI. Our study included 34 svMCI patients and 34 normal controls (NCs), with analysis of T1 images and Montreal Cognitive Assessment (MoCA) scores. Gray matter volume (GMV) of hippocampal sub-regions was quantified and compared between the groups, adjusting for age, sex, and education. Additionally, we explored correlations between altered GMV in hippocampal sub-fields and MoCA scores in svMCI patients. Patients with svMCI exhibited selectively reduced GMV in several left hippocampal sub-regions, such as the hippocampal tail, hippocampal fissure, CA1 head, ML-HP head, CA4 head, and CA3 head, as well as decreased GMV in the right hippocampal tail. Specifically, GMV in the left CA3 head was inversely correlated with MoCA scores in svMCI patients. Our findings indicate that the atrophy pattern of patients with svMCI was predominantly located in the left hippocampal sub-regions. The left CA3 might be a crucial area underlying the distinct pathophysiological mechanisms of cognitive impairments with subcortical vascular origins.
A pilot randomized controlled trial of an online intervention for Hodgkin lymphoma survivors to increase knowledge about late effects and recommended screening
Authors: Rick Tara J. , Sagaram Smitha , Jewett Patricia I. , Lee Hee Yun , Sadak Karim T. , Turcotte Lucie M. , Vogel Rachel I. , Blaes Anne 
Published: April 2024

Abstract
Background
Hodgkin lymphoma (HL) survivors who received chest radiotherapy are at risk for breast cancer and cardiovascular disease, but screening adherence is low. We assessed the acceptability/feasibility of a web-based educational intervention and its impact on knowledge of health risks and screening.

Methods
HL survivors were randomized to either an interactive online educational intervention or handouts only. Surveys were completed at baseline and 3 months post-intervention. We described the acceptability/feasibility of the intervention and compared knowledge between groups.

Results
Fifty-two HL survivors participated; 27 in the intervention group and 25 in the control group. Eighteen (66%) intervention participants completed the intervention and reported high acceptability (89–100%). At baseline, adherence to breast cancer screening was low across all participants. Post-intervention, those in the intervention group more often than controls correctly identified breast cancer and echocardiogram screening guidelines (35% vs. 28%, P = 0.02 and 82% vs. 52%, P = 0.04) and reported knowing how to address potential complications from cancer treatments (87% vs. 64%, P = 0.03). We detected no increase in screening behavior post-intervention.

Conclusion
Online education modules for high-risk HL survivors are an acceptable method to improve knowledge of health risks and screening guidelines. Future interventions should focus on improving screening uptake in this population.

Implications for Cancer Survivors
Web-based learning can be useful in increasing cancer survivor knowledge of their unique risks and screening recommendations but does not necessarily change patient behavior. Involvement in a cancer survivorship program can help assess individual barriers and monitor uptake of screening.
Setting up a CFD model to evaluate the impact of green infrastructures on local air quality
Authors: Rodrigues V. , Augusto B. , Oliveira K. , Ascenso A. , Rafael S. , Nascimento D. , Miranda A. I. 
Published: April 2024

AbstractGreen infrastructures have been pointed out as innovative solutions to deal with current and future challenges related to air pollution and climate change. Although the potential of green infrastructures, such as green walls and green roofs, to mitigate air pollution has been documented, evidence at a local scale is still limited. This work aims to increase knowledge about the potentialities of green infrastructures in improving local air quality, focusing on particulate matter, nitrogen dioxide and ozone pollutants, and by using a local-scale computational fluid dynamics model. The ENVI-met model was applied to a particular hour of a summer day over a built-up environment centred on a main avenue in the city of Lisbon (Portugal). The dimensions of the computational domain are 618 m × 594 m × 143 m, and it contains 184 buildings, with the tallest building being 56 m. In addition to the baseline simulation, modelling was also done considering the application of green walls and green roofs to specific buildings located near the main avenue, together with a green corridor. The overall results show no disturbances exerted by green walls on the turbulent flow dynamics and on the air quality levels when compared to the baseline scenario (without green walls). The integrated scenario, which includes green walls, green roofs and a green corridor, will lead to potential local benefits of green infrastructures on O3 concentrations, followed by variable impacts on NO2 and particulate matter concentrations.
Outcomes of Percutaneous Revascularization in Severe Ischemic Left Ventricular Dysfunction
Authors: Bista Roshan , Zghouzi Mohamed , Jasti Manasa , Lichaa Hady , Kerrigan Jimmy , Haddad Elias , Alraies M. Chadi , Paul Timir K. 
Published: April 2024

Abstract
Purpose of Review
This article presents a comprehensive review of coronary revascularization versus optimal medical therapy (OMT) in patients with severe ischemic left ventricular dysfunction.

Recent Findings
The REVIVED-BCIS2 trial randomized 700 patients with extensive coronary artery disease and left ventricular (LV) ejection fraction (LVEF) ≤ 35% and viability in more than four dysfunctional myocardial segments to percutaneous coronary intervention (PCI) plus OMT versus OMT alone. Over a median duration of 41 months, there was no difference in the composite of all-cause mortality, heart failure hospitalization, or improvement in LVEF with PCI plus OMT versus OMT alone at 6 and 12 months, quality of life scores at 24 months, or fatal ventricular arrhythmia. The STICH randomized trial was conducted between 2002 and 2007, involving patients with LV dysfunction and coronary artery disease. The patients were assigned to either CABG plus medical therapy or medical therapy alone. At the 5-year follow-up, the trial showed that CABG plus medical therapy reduced cardiovascular disease-related deaths and hospitalizations but no reduction in all-cause mortality. However, a 10-year follow-up showed a significant decrease in all-cause mortality with CABG.

Summary
The currently available evidence showed no apparent benefit of PCI in severe ischemic cardiomyopathy as compared to OMT, but that CABG improves outcomes in this patient population. The paucity of data on the advantages of PCI in this patient population underscores the critical need for optimization of medical therapy for better survival and quality of life until further evidence from RCTs is available.
1H, 13C, and 15N backbone and methyl group resonance assignments of ricin toxin A subunit
Authors: Bhattacharya Shibani , Dahmane Tassadite , Goger Michael J. , Rudolph Michael J. , Tumer Nilgun E. 
Published: April 2024

AbstractRicin is a potent plant toxin that targets the eukaryotic ribosome by depurinating an adenine from the sarcin-ricin loop (SRL), a highly conserved stem-loop of the rRNA. As a category-B agent for bioterrorism it is a prime target for therapeutic intervention with antibodies and enzyme blocking inhibitors since no effective therapy exists for ricin. Ricin toxin A subunit (RTA) depurinates the SRL by binding to the P-stalk proteins at a remote site. Stimulation of the N-glycosidase activity of RTA by the P-stalk proteins has been studied extensively by biochemical methods and by X-ray crystallography. The current understanding of RTA’s depurination mechanism relies exclusively on X-ray structures of the enzyme in the free state and complexed with transition state analogues. To date we have sparse evidence of conformational dynamics and allosteric regulation of RTA activity that can be exploited in the rational design of inhibitors. Thus, our primary goal here is to apply solution NMR techniques to probe the residue specific structural and dynamic coupling active in RTA as a prerequisite to understand the functional implications of an allosteric network. In this report we present de novo sequence specific amide and sidechain methyl chemical shift assignments of the 267 residue RTA in the free state and in complex with an 11-residue peptide (P11) representing the identical C-terminal sequence of the ribosomal P-stalk proteins. These assignments will facilitate future studies detailing the propagation of binding induced conformational changes in RTA complexed with inhibitors, antibodies, and biologically relevant targets.