bluuebunny/crossref_metadata_2025_split huggingface dataset:
Facial Paralysis Algorithm: a tool to infer facial paralysis in awake mice
Authors: Perrusquia Hernández Elías , Villeda Arias Diego Israel , Montes Ángeles Claudia Daniela , Andrade González Rey David , Lomelí González Joel , Pérez-Martínez Isaac Obed 
Published: February 2025

Facial paralysis is characterized by an injury to the facial nerve, causing the loss of the functions of the structures that it innervates, as well as changes in the motor cortex. Current models have some limitations for the study of facial paralysis, such as movement restriction, the absence of studying awake animals in behavioral contexts, and the lack of a model that fully evaluates facial movements. The development of an algorithm capable of automatically inferring facial paralysis and overcoming the existing limitations is proposed in this work. In C57/BL6J mice, we produced both irreversible and reversible facial paralysis. Video recordings were made of the faces of paralyzed mice to develop the algorithm for detecting facial paralysis applied to mice, which allows us to predict the presence of reversible and irreversible facial paralysis automatically. At the same time, the algorithm was used to track facial movement during gustatory stimulation, and extracellular electrophysiological recordings in the anterolateral motor cortex. In the basal state, mice can make facial expressions, whereas the algorithm can detect this movement. Simultaneously, such movement is correlated with the activation in the anterolateral motor cortex. In the presence of facial paralysis, the algorithm cannot detect movement. Furthermore, it predicts that the condition exists, and the neuronal activity in the cortex is affected with respect to the evolution of facial paralysis. This way, we conclude that the facial paralysis algorithm applied to mice allows for inferring the presence of experimental facial paralysis and its neuronal correlates for further studies.Significance StatementRecording the faces of mice can help predict facial paralysis unbiasedly and identify the presence of a reduction in facial movement associated with injury to the facial nerve. It can also be used to study facial movements, like facial expressions, and their neural correlates in cortical and subcortical strata. This will not only allow a deep understanding of the magnitude of the effects that facial paralysis can produce at the peripheral and central nervous system levels but also inspire further research and the search for potential treatments.
РЕАЛИЗМ И ХОЛИЗМ НАУЧНОЙ ТЕОРИИ ВОЛНЫ-ПИЛОТА И ФИЛОСОФИИ ДЭВИДА БОМА
Authors: АБРАМОВ П.Д. , НЕКРАСОВА В.М. 
Published: February 2025

Цель работы состоит в том, чтобы раскрыть идеи Бома, прежде всего его представления о холизме и имплицитном порядке, и связать эти философские воззрения с причинной интерпретацией квантовой механики, или теорией де Бройля - Бома. Ключевые принципы причинной интерпретации, или теории скрытых параметров, или волны-пилота, рассматриваются в сопоставлении с принципами копенгагенской интерпретации, исторически первой и одной из наиболее распространенных интерпретаций квантовой механики. Копенгагенская интерпретация и теория де Бройл - Бома содержат разные методологические установки, дающие разные трактовки одних и тех же физических явлений. От феноменологических, не отделяющих «событие» от наблюдения принципов копенгагенской интерпретации отличен реалистический и в онтологическом, и в эпистемологическом плане подход Д. Бома. Представление о нелокальности служит научной основой холистической метафизики Д. Бома. Реальность - это целостность, но целостность, постоянно меняющаяся, в которой имплицитные процессы переходят в эксплицитные, от целостности отделяются относительно автономные и самодостаточные аспекты, составляющие частицы и поля.
The purpose of the paper is to reveal Bohm’s ideas, primarily his notions of holism and implicit order, and to relate these philosophical views to the causal interpretation of quantum mechanics, or the de Broglie-Bohm theory. The key principles of the causal interpretation, or the theory of hidden variables, or the pilot wave theory, are compared with the principles of the Copenhagen interpretation which is historically the first and one of the most widespread interpretations of quantum mechanics. The Copenhagen interpretation and the de Broglie-Bohm theory are based on different methodological approaches, which provide different interpretations of the same physical phenomena. The phenomenological principles of the Copenhagen interpretation, which do not separate the “event” from the observation, differ from Bohm’s approach, realistic in both ontological and epistemological terms. The concept of non-locality serves as the scientific basis for Bohm’s holistic metaphysics. Reality is a whole, but a constantly changing whole, in which implicit processes become explicit, and relatively autonomous and self-sufficient aspects that make up particles and fields separate from the whole.
THE RELATIONSHIP BETWEEN SANITATION PERCEPTION AND THE INCIDENCE OF RESPIRATORY TRACT INFECTION (RTI) IN CORRECTIONAL INSTITUTION
Authors: Satria Hardiat Dani , Budi Musthofa Syamsulhuda , Sakundoro Adi Mateus 
Published: February 2025

Background: The incidence of respiratory tract infection (RTI) in Class IIA Correctional Institution (Lapas) Kendal is notably high. RTI consistently ranks as the most common illness occurring each month, accounting for over 50% of all diseases within the facility. Previous research has indicated that one of the contributing factors to RTI is poor sanitation conditions within the correctional institution. Purpose: This study aims to analyze the relationship between sanitation perceptions and the incidence of RTI in Class IIA Correctional Institution Kendal. Methods: This quantitative study uses a cross-sectional research design with a sample of 294 inmates from the Class IIA Correctional Institution Kendal. The sample was selected using total sampling, involving all inmates in the facility. A Chi-Square test was applied for bivariate analysis. Results: The variable of sanitation perception showed a significant relationship with the incidence of RTI in Class IIA Correctional Institution Kendal. The p-value for the Pearson Chi-Square test was 0.00. Sanitation perception factors included building indicators (p=0.02), room indicators (p=0.54), drinking water indicators (p=0.06), and waste indicators (p=0.00). Conclusion: Sanitation perception factors, such as building indicators and waste indicators, are associated with the incidence of RTI in Class IIA Correctional Institution Kendal. Meanwhile, room indicators and drinking water indicators were not related to the incidence of RTI. To achieve the Sustainable Development Goals (SDGs, it is necessary to implement the Water, Sanitation, and Hygiene (WASH) concept within the correctional institution to minimize the transmission of RTI.
Cultural Humility and Psychedelics: A Framework Analysis of Psychedelic-Assisted Therapy Manuals and Practice Guidelines
Authors: Bell Ayanna K. , Watt Bianca R. , Flores Arturo S. Lopez , Rodriguez Stephanie R. , Smith Darron T. , Stauffer Christopher S. 
Published: February 2025

Abstract

Background
Cultural biases within healthcare systems result in inequitable treatment, harms, and mistrust—particularly for individuals with marginalized identities. Cultural humility training is dynamic, reflective, and emphasizes person-centered care and mutual respect. Psychedelic-assisted therapy (PAT) is an emerging modality for treating psychiatric disorders. Practice manuals and clinical guidelines are crucial for standardizing PAT and for attending to PAT’s unique sensitivity to set and setting, including cultural aspects of care.
Methods
This study investigates the integration of multicultural principles into PAT by analyzing open-source PAT manuals/guidelines against the Multicultural Guidelines established by the American Psychological Association (APA) in 2017. Using framework analysis, two pairs of reviewers assessed the alignment of the PAT manuals/guidelines’ language with the APA guidelines and evaluated their applicability for training therapists to conduct PAT with cultural humility.
Results
Results revealed that while the five manuals/guidelines analyzed exhibit varying degrees of alignment with the guidelines, none provide teachable instructions for delivering culturally responsive care.
Conclusions
Our findings highlight significant gaps in PAT practice manuals and guidelines and underscore the need for a more comprehensive approach to training PAT facilitators. Addressing these shortcomings is essential for advancing equitable PAT for diverse populations. Future efforts should prioritize integrating cultural humility into PAT frameworks to optimize therapeutic outcomes for all.
The role of mutations, addition of amino acids and exchange of genetic information in co-evolution of primitive coding systems
Authors: Pawlak Konrad , Blazej Pawel , Mackiewicz Dorota , Mackiewicz Pawel 
Published: February 2025

Abstract

The standard genetic code (SGC) is a crucial system for encoding biological information but its evolution remains still unresolved and is a topic of debate. Thus, we used a methodology based on the evolutionary algorithm to investigate the emergence of stable coding systems. The simulation started with a population of various primitive genetic codes, which encoded ambiguously only a small number of amino acids (labels). These codes were subjected to a selection process for the accuracy of reading genetic information and increasing coding potential as well as mutation modelled by the changing assignment of the labels to codons, the stepwise introduction of new amino acids into the codes and the exchange of information between the co-evolving coding systems. The evolution converged towards stable and unambiguous coding systems with the higher coding capacity necessary for the production of more diversified proteins. An important factor was the exchange of the encoded information between the evolving codes, which substantially shortened the simulation time to produce the genetic codes encoding 21 labels. The findings highlight key factors that may have shaped the structure of the current genetic code.
High iodine groundwater in the lower Kuitun River in Xinjiang: Evidences from stable carbon isotopes characteristics
Authors: Chao Bo , He Jiale , Luo Yanli , Dong Lele , Zhang Qian , Xie Xinzhe , Wang Meijuan , Song Zhen , Liu Xuan 
Published: February 2025

Abstract

The ubiquitous presence of high-iodine groundwater poses a risk to human health. Degradation of organic matter through microbial activities is an important process of iodine enrichment in groundwater systems. The stable carbon isotope ratios of groundwater have certain indicative significance for understanding the formation process of high-iodine groundwater. This study aimed to explore the role of microbiological processes in enriching iodine in high-iodine groundwater downstream of the Kuitun River in China and employed stable carbon isotopes to assess the influence of organic matter biodegradation on groundwater iodine enrichment. The results showed that all groundwater in our study area exhibited reducing conditions and was weakly alkaline, primarily consisting of slightly saline water with dominant anions and cations being Cl- and Na+, respectively. The concentration of I- in groundwater ranged from 51.66 to 552.79 µg/L, with an average of 177.68 µg/L. Approximately 61.54% of the groundwater was highly enriched in iodine. Dissolved inorganic carbon (DIC) concentration in groundwater ranged from 22.97 to 100.85 mg/L, primarily due to microbial degradation of organic matter and weathering dissolution of silicate minerals, primarily consisting of HCO3-. DOC concentration ranged from 2.01 to 4.22 mg/L, mainly originating from C3 plants. In reducing environments with abundant organic matter in aquifers, microbial involvement in organic matter decomposition and reducible dissolution of iron minerals were the primary hydro-biogeochemical processes leading to the release of solid-phase iodine in aquifers and its migration into groundwater. The model for the origin of high-iodine groundwater in the study area was of the burial-dissolution type.
Armed Conflict and Depression: a comparative longitudinal study in two Colombian populations
Authors: Londoño Jairo Franco , Gomez German Alberto Moreno , Ramirez Oscar Mauricio Castaño , Ramirez Juan Dario Franco , Gomez Mateo Moreno , Gomez Juanita Moreno 
Published: February 2025

Abstract

Background
depressive disorders rank among the most prevalent diseases globally and are the 13th leading cause of years lost due to disability. Exposure to violence, particularly in areas affected by armed conflict, is a significant risk factor for depression. This study examines the prevalence of depressive symptoms in two populations: San Félix (exposed) and San José (non-exposed), across three key periods: before, during, and after the Colombian peace agreement.
Methods
this longitudinal study utilized repeated measures in independent groups. Depressive symptoms were assessed using the Zung and Conde scale in 2004, 2018, and 2020. Univariate and bivariate analyses described population characteristics, while repeated measures ANOVA evaluated differences in depressive symptoms across years and localities.
Results
in 2004, the prevalence of depressive symptoms was 29.8% in San Félix and 23.2% in San José. By 2018, these figures decreased to 17.8% and 14.0%, respectively (p < 0.05). However, by 2020, prevalence increased to 28.4% in San Félix and 15.8% in San José (p = 0.004). A significant association between female sex and depressive symptoms was noted (p = 0.012). Repeated measures analysis indicated statistically significant differences across localities (p = 0.0092), survey years (p = 0.000), and their interaction (p = 0.0002).
Conclusions
this study highlights the association between armed conflict exposure and increased depressive symptoms, with notable prevalence in women. These findings emphasize the need for targeted mental health interventions and support strategies to effectively address the challenges faced by all affected populations in conflict-affected regions.
Cytokine and Oxidative Stress Imbalances in Relation to Complex Coronary Lesions in Elderly Patients
Authors: Li Xia , zhao Yongjuan , Zhou Hualan , Hu Youdong , Chen Ying , Guo Dianxuan 
Published: February 2025

Background:
Complex coronary lesions have been an understudied aspect of coronary
artery disease in elderly patients. Oxidative stress and inflammation may be implicated in the pathogenesis
of complex coronary lesions.

Objective:
The aim of this study is to investigate the complex interplay between pro-oxidative stress
response, pro-inflammatory response, and complex coronary lesions in elderly patients.

Methods:
Enzyme-linked immunosorbent assays for the detection of serum biomarkers [reactive
oxygen species (ROS), malondialdehyde (MDA), tumor necrosis factor-α (TNF-α), interferon-gamma
(IFN-γ), superoxide dismutase (SOD) activity, total antioxidant capacity (TAC), transforming growth
factor beta (TGF-β) and interleukin-4 (IL-4)] were performed in elderly patients with complex coronary
lesions.

Results:
The levels of pro-oxidative stress and pro-inflammatory markers (ROS, MDA, TNF-α and
IFN-γ) were increased in the complex coronary lesion group when compared with the non-complex
coronary lesion group (P < 0.01) in elderly patients. Anti-oxidative stress and anti-inflammatory
markers (SOD activity, TAC, TGF-β, and IL-4) were decreased in the complex coronary lesion group
when compared with the non-complex coronary lesion group (P < 0.01) in elderly patients.

Conclusion:
Our findings suggest that the pathogenesis of complex coronary lesions may involve
pro-oxidant/anti-oxidant and pro-inflammation/anti-inflammation imbalance, as well as the interplay
between oxidative stress and inflammation in elderly patients.

other:
No
Synergistic Warriors: Design and Synthesis of Dual-Acting Schiff-Based Threaded 1,2,3-Triazole Hybrids for Potent Antineoplastic and Anti-Mycobacterial Activities
Authors: Walhekar Vinayak , Kulkarni Raghavendra , Nuli Mohana Vamsi , Garige Anil Kumar , Deore Dharmesh , Pawar Ritesh , Patil Ashwini , DVRN Bhikshapathi , Santosh Karajagi , Kulkarni Ravindra 
Published: February 2025

Objective:
In the pursuit of identifying divergent scaffolds for potential anticancer and
anti-mycobacterial agents, a novel series of Schiff-based threaded 1,2,3-triazoles was designed and
synthesized.

Methods:
In this study, novel Schiff-based threaded 1,2,3-triazoles have been meticulously crafted
and synthesized. Chemical structures of the synthesized molecules were confirmed by 1H NMR,
13C NMR and Mass spectra. Synthetic analogs were further evaluated for their antiproliferative,
antitubercular and antimicrobial potentials by in vitro assays.

Results:
The in vitro anti-tumor (anti-proliferative) evaluation on HT29 cancer cells revealed that
compounds 8b and 8h exhibited remarkable inhibitory activity with IC50 values of 25±0.8 and
24±0.9 μM. In the context of anti-mycobacterial analysis, compound 8c demonstrated promising
activity (6.25 μM) against Mycobacterium tuberculosis H37Rv. Moreover, compounds 8d and 8e
displayed equipotent antimicrobial potential (3.12 μM) comparable to Ciprofloxacin against both
Staphylococcus aureus and Escherichia coli. Molecular docking studies unveiled that 8c exhibited
robust binding within the active pocket of carbonic anhydrase XII (docking energy -8.4 kcal/mol)
and demonstrated a promising docking profile with β-ketoacyl ACP synthase I (docking energy -
9.5 kcal/mol) in the enzyme's binding pocket.

Conclusion:
Structure-activity relationship (SAR) analysis identified three pivotal pharmacophores;
1,2,3-triazole, aromatic ring system (substituted with halogens and -NO2), and imine functionalities
as crucial for the development of dual inhibitors targeting cancer and tuberculosis, showcasing
an outstanding in silico ADMET profile. Therefore, these compounds merit consideration as
noteworthy pharmacological lead molecules in the realm of cancer and tuberculosis drug discovery
and development.
Transporter Associated with Antigen Processing
Proteins (TAP-1 and TAP-2) Gene Expression
of MHC-I Downregulated in Oral Squamous
Carcinoma
Authors: Singh Vijay , Dwivedi Shailendra , Agrawal Ruchika , PS Mohan Raj , Bansal Akash , Agarwal Akash , Misra Sanjeev 
Published: February 2025

Background:
TAP-1 and TAP-2 are crucial proteins for loading antigenic peptides after
proteasome-mediated endogenous processing of the MHC-I (Major Histocompatibility Complex-
I) pathway. Our study aimed to explore the Transporter Associated with Antigen Processing
proteins (TAP-1 and TAP-2) in oral squamous cell carcinoma and premalignant oral lesions.

Methods:
We recruited a total of 135 subjects from the outpatient department of the ENT unit of
our institute. Real-time Polymerase Chain Reaction (PCR) was used to evaluate the levels of
TAP-1 and TAP-2 gene expression in pre-cancerous and oral squamous carcinoma samples. Additionally,
we measured the circulating levels of inflammatory markers using an automated biochemistry
analyzer.

Results:
In the current study, we found that the subjects with oral squamous cell carcinoma had
lower expressions of the TAP 1 and TAP 2 genes than precancerous oral subjects of OSMF, leukoplakia,
and OLP. In oral squamous carcinoma subjects, we found a 1.7- and 2.1-fold change in
gene expression of TAP-1 and TAP-2, respectively, compared to control subjects. Furthermore,
we observed an increase in levels of metabolic inflammatory biomarkers of CRP, ESR, and ferritin
in oral squamous carcinoma subjects compared to premalignant cases and controls, indicating
the presence and aggravation of systemic inflammation.

Conclusion:
The study revealed that subjects with oral squamous cell carcinoma have lower TAP
1 and TAP 2 gene expression than premalignant control subjects, thus affecting MHC-I processing,
which ultimately affects the functioning of the immune system. These results have the potential
to improve our understanding of disease pathophysiology and provide more targeted treatment
options.
Halo-tolerant Plant Growth Promoting Rhizobacteria Enhancing Mustard Seed Growth Under Salinity Stress for Sustainable Agriculture
Authors: Sharma Kusum , Sharma Pawan Kumar , Prasad S Rajendra , Singh Anupam , Jain Divya , Singh Sachidanand 
Published: February 2025

Introduction:
Salinity is one of the primary environmental factors that significantly
impact global crop production. Plant growth promoting rhizobacteria (PGPR) inoculation
to crops improves the productivity of the crops.

Method:
To develop a biofertilizer specifically for saline soil, bacteria were isolated from
the rhizosphere of mustard plants along with the plant growth-promoting traits grown in
saline soil (EC 6 dS m). Halotolerant 22 bacterial strains were isolated and identified
from the rhizospheric soil mustard crop, Purvanchal (Indian state). According to the
study, 54.54% of the isolates had phosphate solubilization efficiencies ranging from 7%
to 27% on plate assays. According to quantitative measurements, 63.63% of the strains
exhibited the ability to solubilize phosphate, with degrees of solubilization varying between
0.49 and 3.34 μg/ml. Furthermore, 50% of the isolates showed the ability to solubilize
zinc, with solubilization rates varying from 12% to 53%. Further 59.09% of the
bacterial strains showed ammonium production test; these strains were classified as having
low (+), medium (++), and high (+++) levels of ammonium production.

Result:
According to the research, these halo-tolerant plant growth-promoting rhizobacteria
(PGPR) have particular functional properties that may help mustard crops grow
more rapidly in salinity-stressed environments. Because these PGPR strains increase nutrient
availability and stimulate plant development, they may find use in agriculture, especially
in saline settings.

Conclusion:
The study emphasizes how crucial it is to use PGPR with particular nutrient
mobilization features to promote crop growth under difficult circumstances.