bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of human NLZ1/ZNF703 identifies conserved domains essential for proper subcellular localization and transcriptional repression
Authors: Pereira‐Castro Isabel , Costa Ângela M. Sousa , Oliveira Maria José , Barbosa Inês , Rocha Ana Sofia , Azevedo Luisa , da Costa Luís Teixeira 
Published: August 2012

AbstractNET family members have recently emerged as important players in the development of multiple structures, from the trachea of fly larvae to the vertebrate eye and human breast cancers. However, their mechanisms of action are still poorly understood, and we lack a detailed characterization of their functional domains, as well as gene expression patterns—particularly in adult mammals. Here, we present a characterization of human NLZ1/ZNF703 (NocA‐like zinc finger 1/Zinc finger 703), one of the two human NET family member genes. We show that the gene is ubiquitously expressed in adult human and mouse tissues, that three mRNA species with the same coding sequence are generated by alternative polyadenylation, and that the encoded protein contains six evolutionarily conserved domains, three of which are specific to NET proteins. Finally, we present functional evidence that these domains are necessary for proper subcellular distribution of and transcription repression by the NLZ1 protein, but not for its interaction with Groucho family co‐repressors. J. Cell. Biochem. 114: 120–133, 2012. © 2012 Wiley Periodicals, Inc.
Differentiation potential and GFP labeling of sheep bone marrow‐derived mesenchymal stem cells
Authors: Czernik Marta , Fidanza Antonella , Sardi Martina , Galli Cesare , Brunetti Dario , Malatesta Daniela , Della Salda Leonardo , Matsukawa Kazutsugu , Ptak Grazyna E. , Loi Pasqualino 
Published: August 2012

AbstractMesenchymal stem cells (MSCs) are an important cell population in the bone marrow microenvironment. MSCs have the capacity to differentiate in vitro into several mesenchymal tissues including bone, cartilage, fat, tendon, muscle, and marrow stroma. This study was designed to isolate, expand, and characterize the differentiation ability of sheep bone marrow‐derived MSCs and to demonstrate the possibility to permanently express a reporter gene. Bone marrow was collected from the iliac crest and mononuclear cells were separated by density gradient centrifugation. Sheep MSCs cell lines were stable characterized as CD44+ and CD34− and then transfected with a green fluorescent protein (GFP) reporter gene. The GFP expression was maintained in about half (46.6%) of cloned blastocysts produced by nuclear transfer of GFP+ sheep MSCs, suggesting the possibility to establish multipotent embryonic cells' lines carrying the fluorescent tag for comparative studies on the differentiation capacity of adult stem cells (MSCs) versus embryonic stem cells. We found that sheep MSCs under appropriate culture conditions could be induced to differentiate into adipocytes, chondrocytes, and osteoblast lineages. Our results confirm the plasticity of sheep MSCs and establish the foundation for the development of a pre‐clinical sheep model to test the efficiency and safety of cell replacement therapy. J. Cell. Biochem. 114: 134–143, 2012. © 2012 Wiley Periodicals, Inc.
The release of glutamate from cortical neurons regulated by BDNF via the TrkB/Src/PLC‐γ1 pathway
Authors: Zhang Zitao , Fan Jin , Ren Yongxin , Zhou Wei , Yin Guoyong 
Published: August 2012

AbstractThe brain‐derived neurotrophic factor (BDNF) participates in the regulation of cortical neurons by influencing the release of glutamate. However, the specific mechanisms are unclear. Hence, we isolated and cultured the cortical neurons of Sprague Dawley rats. Specific inhibitors of TrkB, Src, PLC‐γ1, Akt, and MEK1/2 (i.e., K252a, PP2, U73122, LY294002, and PD98059, respectively) were used to treat cortical neurons and to detect the glutamate release from cortical neurons stimulated with BDNF. BDNF significantly increased glutamate release, and simultaneously enhanced phosphorylation levels of TrkB, Src, PLC‐γ, Akt, and Erk1/2. For BDNF‐stimulated cortical neurons, K252a inhibited glutamate release and inhibited the phosphorylation levels of TrkB, Src, PLC‐γ, Erk1/2, and Akt (P < 0.05). PP2 reduced the glutamate release from BDNF‐stimulated cortical neurons (P < 0.05) and inhibited the phosphorylation levels of TrkB and PLC‐γ1 (P < 0.05). However, PP2 had no effect on the phosphorylation levels of Erk1/2 or Akt (P > 0.05). U73122 inhibited the glutamate release from BDNF‐stimulated cortical neurons, but had no influence on the phosphorylation levels of TrkB, Src, Erk1/2, or Akt (P > 0.05). LY294002 and PD98059 did not affect the BDNF‐stimulated glutamate release and did not inhibit the phosphorylation levels of TrkB, Src, or PLC‐γ1. In summary, BDNF stimulated the glutamate release from cortical neurons via the TrkB/Src/PLC‐γ1 signaling pathway. J. Cell. Biochem. 114: 144–151, 2012. © 2012 Wiley Periodicals, Inc.
Galangin inhibits tumor growth and metastasis of B16F10 melanoma
Authors: Zhang Wenjing , Tang Bo , Huang Qilai , Hua Zichun 
Published: August 2012

AbstractGalangin, an active flavonoid extracted from the root of the Alpinia officinarum Hance, showed a cytotoxic effect on several cancer cell lines in vitro. However, there is no information available concerning its antimetastatic effect. Focal adhesion kinase (FAK), a cytoplasmic tyrosine kinase, is involved in many aspects of cellular processes such as proliferation, adhesion, and invasion. Studies have shown that FAK is a promising target for therapeutic intervention in melanoma. In the present study, proliferation of B16F10 cells was suppressed when exposed to various doses of galangin. Inhibition on proliferation by galangin was also detected by clonogenic survival assay. The capabilities of cell adhesion, cell spreading, and cell motility were impaired by galangin, reinforced by F‐actin rearrangement. Molecular data showed that both FAK mRNA level and protein level were reduced dose‐dependently. Additionally, galangin reduced phosphorylation of FAK (Tyr397) protein. Transient transfection reporter assays showed that galangin suppressed the transcription of FAK gene, indicating FAK expression is a candidate target of galangin. The antimetastatic function of galangin is further supported by the fact that it could inhibit the formation of tumor colonies in the lung tissue on C57BL/6J mouse lung metastatic model using B16F10 melanoma cells. Immunochemical analyses showed that galangin decreased FAK expression in vivo. These data add to our new understanding that galangin can inhibit B16F10 melanoma metastasis both in vivo and in vitro, and that FAK is a valid therapeutic target against melanoma. J. Cell. Biochem. 114: 152–161, 2012. © 2012 Wiley Periodicals, Inc.
Response of mice connective tissue to intracanal dressings containing chlorhexidine
Authors: Pereira Maristela Soares Swerts , Faria Gisele , Bezerra Da Silva Léa Assed , Tanomaru‐Filho Mário , Kuga Milton Carlos , Rossi Marcos A. 
Published: August 2012

AbstractSubstances containing chlorhexidine (CHX) have been studied as intracanal medicaments. The aim of the present study was to characterize the response of mouse subcutaneous connective tissue to CHX‐containing medications by conventional optical microscopy. The tissue response was evaluated by implanting polyethylene tubes containing one of the substances evaluated: Calen paste + 0.5% CHX, Calen + 2% CHX, 2% CHX gel, and Calen paste (control). After experimental periods of 7, 21, and 63 days, the implants (n = 10) were removed along with the subcutaneous connective tissue. Tissue samples were subjected to histological processing, and sections were stained with hematoxylin and eosin. Qualitative and quantitative analyses of the number of inflammatory cells, blood vessels, and vascularized areas were performed. Results were analyzed by ANOVA and Tukey tests with the significance level set at 5%. We concluded that Calen + 0.5% CHX led to reparative tissue response in contrast with Calen + 2% CHX and 2% CHX gel, which induced persistent inflammatory response, pointing to the aggressive nature of this mixture. When Calen + 2% CHX and 2% CHX gel were compared, the latter induced more intense inflammatory response. Microsc. Res. Tech., 2012. © 2012 Wiley Periodicals, Inc.
A multipartite Ramsey number for odd cycles
Authors: Benevides Fabrıcio Siqueira 
Published: August 2012

AbstractIn this article we study multipartite Ramsey numbers for odd cycles. Our main result is the proof that a conjecture of Gyárfás et al. (J Graph Theory 61 (2009), 12–21), holds for graphs with a large enough number of vertices. Precisely, there exists n0 such that if n⩾n0 is a positive odd integer then any two‐coloring of the edges of the complete five‐partite graph K(n − 1)/2, (n − 1)/2, (n − 1)/2, (n − 1)/2, 1 contains a monochromatic cycle of length n. © 2011 Wiley Periodicals, Inc. J Graph Theory
On r‐Connected Graphs with No Semi‐topological r‐Wheel
Authors: Horev Elad , Lomonosov Michael 
Published: August 2012

AbstractA semi‐topological r‐wheel, denoted by , is a subdivision of the r‐wheel preserving the spokes. This paper describes the r‐connected graphs having no ‐subgraphs. For , these are shown to be only , while the class of 3‐connected ‐free graphs is unexpectedly rich. First, every graph G in has an efficiently recognizable set of “contractible edges” (sometimes empty) such that a contraction minor belongs to if and only if F is a part of this set. So, the subclass of ante‐contraction members of plays a key role. Second, the members of have 3‐edge cuts. The familiar cactus representation of minimum edge cuts (Dinits et al., in: Issledovaniya po Diskretnoy Optimizatsii, Nauka, Moscow, pp. 290–306, 1976 (Russian); also A. Schrijver, Combinatorial Optimization (Polyhedra and Efficiency), Algorithms and Combinatorics, Vol. 24, Springer, 2003, p. 253) maps onto the class of trees whose internal vertices have even degrees, equal to 6 for any vertex adjacent to a leaf. The description of (quite concise as expressed in appropriate terms) refers to the explicit reconstruction of the reverse image of such a tree. We also derive the upper bound on the number of edges in an arbitrary n‐vertex ‐free graph, , and conjecture that its maximum equals .
The Number of Subtrees of Trees with Given Degree Sequence
Authors: Zhang Xiu‐Mei , Zhang Xiao‐Dong , Gray Daniel , Wang Hua 
Published: August 2012

AbstractThis article investigates some properties of the number of subtrees of a tree with given degree sequence. These results are used to characterize trees with the given degree sequence that have the largest number of subtrees, which generalize the recent results of Kirk and Wang (SIAM J Discrete Math 22 (2008), 985–995). These trees coincide with those which were proven by Wang and independently Zhang et al. (2008) to minimize the Wiener index. We also provide a partial ordering of the extremal trees with different degree sequences, some extremal results follow as corollaries.
Relations between ENSO and the South Atlantic SST modes and their effects on the South American rainfall
Authors: Kayano Mary T. , Andreoli Rita V. , Ferreira de Souza Rodrigo A. 
Published: August 2012

AbstractThis paper analyses the relations between the El Niño/Southern Oscillation (ENSO) mode in the tropical Pacific and the sea surface temperature (SST) modes in the South Atlantic for the 1900–2006 period. In the South Atlantic, two modes are analysed in more detail: the South Atlantic dipole (SAD) mode, with centres at (15°S, Greenwich longitude) and at (37.5°S, 25°W) and the southwestern South Atlantic (SWSA) mode, with centres at (30°S, 40°W) and at the southern midlatitudes. The ENSO and SAD modes are lagged or lead connected depending on the period of analysis. An El Niño (a La Niña) precedes by up to 6 months the establishment of a positive (negative) SAD mode during the 1920–1930 and 1940–1980 periods. Otherwise, a positive (negative) SAD mode precedes by up to 1 year the establishment of a La Niña (El Niño) during the 1975–2000 period. On the other hand, the SWSA is strongly driven by the ENSO. The effects of both the SAD and SWSA modes on the South American rainfall are also discussed on a seasonal basis. In general, the SAD (ENSO) mode has a weak influence on the ENSO‐related (SAD‐related) rainfall anomalies over South America. On the other hand, in general, the SWSA and ENSO modes have a combined effect on rainfall of the southern and southeastern regions of South America. A particularly important result of the present analysis for climate monitoring and forecasting purposes seems to be the changing relations between the SAD and ENSO modes. Copyright © 2012 Royal Meteorological Society
High‐anthocyanin strawberries through cultivar selection
Authors: Fredericks Charissa H , Fanning Kent J , Gidley Michael J , Netzel Gabriele , Zabaras Dimitrios , Herrington Mark , Netzel Michael 
Published: August 2012

AbstractBACKGROUND: Diets high in fruit and vegetables are known to have significant health benefits. This is in part due to the presence of phytochemicals, which possess potential protective health benefits. This study focuses on the ability of strawberries to be bred for higher anthocyanin content. This is a major contributor to the characteristic colour and nutritional value of ripe strawberries, together with phenolic acids, ascorbic acid and total antioxidant capacity.RESULTS: Anthocyanins in five commercial strawberry cultivars and three breeding lines were assessed. This led to the discovery of one breeding line (BL 2006‐221) as an exceptional source of anthocyanins (∼1 g kg−1 fresh weight), with approximately double the levels of current commercial cultivars. Temperature was shown to influence anthocyanin extraction, with 40 °C being the best extraction temperature using the accelerated solvent extraction (ASE) method. Hue angle and anthocyanin concentration showed a good correlation (r2 = 0.69).CONCLUSION: The new breeding line BL 2006‐221 has the potential to be used in the development of phytochemically rich strawberry cultivars. Using hue angle as a screening tool for total anthocyanin concentration and extraction of anthocyanins from strawberries by ASE at 40 °C would support such cultivar development. © 2012 Commonwealth of Australia
An environmentally friendly and cost effective technique for the commercial cultivation of oyster mushroom [Pleurotus florida (Mont.) Singer]
Authors: Panjikkaran Seeja Thomachan , Mathew Deepu 
Published: August 2012

AbstractBACKGROUND: The existing protocol for the cultivation of oyster mushroom (Pleurotus florida) in polyethylene bags leads to environmental pollution amounting to 18 g of polyethylene per 450 g of mushroom, which is the average biological efficiency achieved from a bag. Thus the projected annual global pollution amounts to 2 million tones, corresponding to the production of 48 million tones. Experiments were conducted at Kerala Agricultural University, India, to formulate an oyster mushroom cultivation strategy that reduces this pollution level.RESULTS: Pooled results of experiments at the institute's and farmers' units have shown that reusable plastic buckets having perforations of 1.5 cm × 1.0 cm throughout the side walls could be used to substitute polyethylene bags, while following the standard cultivation protocols. Cultivation in perforated buckets has recorded a biological efficiency of 435.69 ± 56.75 g in 47.07 ± 5.22 days against 459.11 ± 53.52 g in 38.05 ± 4.54 days in polyethylene bags. The rate of contamination in buckets was significantly lower than that in bags: 9.28 ± 2.12 and 12.60 ± 3.73% respectively.CONCLUSION: Reusable plastic buckets with perforations on the side walls could be used to substitute the conventional polyethylene bags in oyster mushroom cultivation, with no significant difference in yield. Losses due to slight increase in crop duration in buckets will be compensated with a lower rate of contamination. For a unit having a daily output of 100 kg, it was estimated that during 10 years of permanent cultivation following this technique, the cost of cultivation could be reduced to one‐tenth and the environmental pollution reduced by at least 730 000 non‐degradable polyethylene bags. © 2012 Society of Chemical Industry
Immunological determination of gliadin 33‐mer equivalent peptides in beers as a specific and practical analytical method to assess safety for celiac patients
Authors: Comino Isabel , Real Ana , de Lourdes Moreno Maria , Montes Raquel , Cebolla Ángel , Sousa Carolina 
Published: August 2012

AbstractBACKGROUND: Cereals used for beer manufacturing contain gluten, which is immunotoxic for celiac patients. The gluten remaining after processes of malting and brewing is mostly hydrolyzed, which makes practical evaluation of the immunotoxicity of the gluten pools challenging.RESULTS: We analyzed the presence of gluten peptides equivalent to the major immunotoxic protease‐resistant gliadin 33‐mer in 100 Belgium beers, using monoclonal antibodies (G12/A1). Immunochromatographic strips and enzyme‐linked immonosorbent assay G12/A1 methods estimated at least 20 ppm gluten equivalents in 90 beers and gluten‐free in 10 beers. The G12/A1 reactivity of beer high‐performance liquid chromatographic fractions correlated to the presence of T‐cell‐reactive epitopes identified by peptide sequencing.CONCLUSION: The determination of equivalent gliadin 33‐mer epitopes in beers has been shown to be practical, specific, and sensitive for the measurement of potential immunotoxicity for celiac patients. Copyright © 2012 Society of Chemical Industry
Adjuvant therapy for pancreas adenocarcinoma
Authors: O'Reilly Eileen M. 
Published: August 2012

AbstractAdjuvant therapy for pancreas adenocarcinoma in 2012 includes consideration of systemic therapy based on high level evidence and combined chemoradiotherapy based on less robust data. Current major adjuvant questions are examining the role of the addition of a second agent, either cytotoxic or targeted agent, to gemcitabine and whether or not the utilization of combined chemoradiotherapy improves overall survival. Progress to date has been modest and incremental in the adjuvant setting. J. Surg. Oncol. 2013;107:78–85. © 2012 Wiley Periodicals, Inc.
Podoplanin expression in cutaneous head and neck squamous cell carcinoma—prognostic value and clinicopathologic implications
Authors: Kreppel Matthias , Krakowezki Anton , Kreppel Barbara , Drebber Uta , Wedemeyer Inga , Mauch Cornelia , Zöller Joachim E. , Scheer Martin 
Published: August 2012

AbstractBackgroundAlthough most patients are cured by local treatment from cutaneous head and neck squamous cell carcinoma (cHNSCC), a significant number of patients develops metastases to the regional lymph nodes. Recent studies suggest an influence of podoplanin expression on regional lymph node metastases in other head and neck squamous cell carcinoma. The aim of our study was to assess the impact of podoplanin expression on regional lymph node metastasis, locoregional recurrence, and prognosis.MethodsIn this retrospective study, podoplanin expression was examined immunohistochemically in 63 treatment‐naive patients with cHNSCC. We analyzed associations of podoplanin expression and clinicopathologic variables. Furthermore, we investigated the effects on overall survival (OS) and locoregional control in univariate and multivariate analysis.ResultsIn 40 patients (63.5%), podoplanin was expressed in the tumor cells. The χ2‐test revealed that podoplanin expression was associated with the number of tumorous lymph nodes (P < 0.001). The OS was significantly influenced by podoplanin expression (P < 0.001). None of the patients with high levels of podoplanin expression survived, whereas the 5‐year OS for patients with podoplanin‐negative tumors was 91.3%.ConclusionsWe concluded that podoplanin is frequently expressed in cHNSCC and might serve as predictor for regional lymph node metastases, locoregional recurrence, and clinical outcome. J. Surg. Oncol. 2013;107:376–383. © 2012 Wiley Periodicals, Inc.
High‐temperature kinetics of the reaction between CN and hydrocarbons using a novel high‐enthalpy flow tube
Authors: Gardez Aline , Saidani Ghassen , Biennier Ludovic , Georges Robert , Hugo Edouard , Chandrasekaran Vijayanand , Roussel Vivien , Rowe Bertrand , Reddy K. P. J. , Arunan E. 
Published: August 2012

AbstractMore than 70 molecules of varied nature have been identified in the envelopes of carbon‐rich stars through their spectral fingerprints in the microwave or far infrared regions. Many of them are carbon chain molecules and radicals, and a significant number are unique to the circumstellar medium. The determination of relevant laboratory kinetics data is critical to keep up with the development of the high spectral and spatial resolution observations and of the refinement of chemical models. Neutral–neutral reactions of the CN radical with unsaturated hydrocarbons could be a dominant route in the formation of cyanopolyynes, even at low temperatures and deserve a detailed laboratory investigation. The approach we have developed aims to bridge the temperature gap between resistively heated flow tubes and shock tubes. The present kinetic measurements are obtained using a new reactor combining a high‐enthalpy source with a flow tube and a pulsed laser photolysis–laser‐induced fluorescence system to probe the undergoing chemical reactions. The high‐enthalpy flow tube has been used to measure the rate constant of the reaction of the CN radical with propane (C3H8), propene (C3H6), allene (C3H4), 1,3‐butadiene (1,3‐C4H6), and 1‐butyne (C4H6) over a temperature range extending from 300 to 1200 K. All studied reactions of CN with unsaturated hydrocarbons are rapid, with rate coefficients greater than 10−10 cm3 · molecule−1 · s−1 and exhibit slight negative temperature dependence above room temperature. © 2012 Wiley Periodicals, Inc. Int J Chem Kinet 44: 753–766, 2012
Mechanical properties of porcine cartilage after uniform RF heating
Authors: Zemek Allison J. , Protsenko Dmitry E. , Wong Brian J.F. 
Published: August 2012

AbstractBackground and ObjectivesThermally mediated modalities of cartilage reshaping utilize localized heating of cartilage combined with mechanical deformation to achieve new geometries. We sought to determine the steady state elastic modulus of thermally modified cartilage without deformation, as this provides a constraint in mechanical models of the shape change process.Study Design/Materials and MethodsThe main objective of this study was to characterize the steady state elastic modulus of porcine septal cartilage after uniform heating with radiofrequency (RF) to peak temperatures of 50 ± 5, 65 ± 5, and 85 ± 5°C. The cartilage was divided into three equally sized regions, designated as anterior, middle and posterior. Each region was then sectioned into two specimens with the proximal component serving as a paired control.ResultsThe data confirm that there is high baseline variability in control steady state elastic moduli between animals. Also, the control values confirm a decreasing steady state elastic modulus from anterior to posterior. There is no statistical significance (P > 0.05) found between the elastic moduli of control and treated samples.ConclusionsAlthough shape change and retention have been fairly well characterized, little is known about the specific relation between steady state elastic modulus of cartilage and maximum treatment temperature. We determined that the difference of steady state elastic modulus between control and treated porcine septal samples was not statistically significant after uniform heating with RF to peak temperatures of 50 ± 5, 65 ± 5, and 85 ± 5°C. Ultimately, the results of this study do not pertain to the regions of heated cartilage that are shaped to hold a new form; however, it does show that the regions that are not mechanically deformed do return to the original pre‐treatment elastic modulus. This is still useful information that may be used in finite element models to predict changes in internal stress distributions of cartilage after laser reshaping. Lasers Surg. Med. 44: 572–579, 2012. © Wiley Periodicals, Inc.