bluuebunny/crossref_metadata_2025_split huggingface dataset:
‘I am absolutely shattered’: The impact of chronic low back pain on Australian Aboriginal people
Authors: Lin I.B. , O'Sullivan P.B. , Coffin J.A. , Mak D.B. , Toussaint S. , Straker L.M. 
Published: March 2012

AbstractBackgroundAboriginal people in Australia have been uniquely identified as less susceptible to chronic low back pain (CLBP) disability when compared to non‐Aboriginal populations, reportedly due to cultural beliefs about pain. A qualitative, culturally secure research approach was used to explore this assumption.MethodsIn‐depth interviews were undertaken with 32 Aboriginal men and women with CLBP in regional and remote areas of Western Australia. Interviews were conducted collaboratively with male and female Aboriginal co‐investigators, and with the support of local Aboriginal community organizations. A primary focus was to investigate the impact of CLBP from the perspective of Aboriginal people living with the condition.ResultsThe experience of CLBP was found to be multidimensional, impacting on activities of daily life, employment, sport and family participation, emotional and cultural well‐being.ConclusionsContrary to previous assumptions, CLBP is profoundly disabling for some Aboriginal people and a priority health concern. Issues of gender, cultural obligations and the emotional consequences of CLBP are important consideration for health care. These findings, and the contextual approach used to gain an in‐depth understanding of CLBP, may be relevant to populations elsewhere.
Effects of dexmedetomidine on conditioned pain modulation in humans
Authors: Baba Y. , Kohase H. , Oono Y. , Fujii‐Abe K. , Arendt‐Nielsen L. 
Published: March 2012

AbstractBackgroundSystemic administration of dexmedetomidine (DEX; selective α2‐adrenoceptor agonist) is found to inhibit diffuse noxious inhibitory control in rats, now referred to as conditioned pain modulation (CPM) in humans. The present study was designed to investigate the effect of intravenous administration of DEX on CPM in humans.MethodsThere were two sequential sessions in this double blind, randomized study. The first session was the control with normal saline infusion (N1st, L1st, H1st). During the second session, three types of agents were infused: normal saline (N2nd); a low plasma concentration of DEX (0.04 ng/mL; L2nd); and a high plasma concentration of DEX (0.08 ng/mL; H2nd).The amplitude of somatosensory evoked potentials (ampSEP)s and the visual analogue scale of tooth pain (VASt) induced by electrical tooth stimulation were evaluated with and without conditioning CO2 laser stimulation of the hand. The inhibition rate (% inhibition) was calculated [= (1‐[ampSEP or VASt with conditioning stimuli]/[ampSEP or VASt without conditioning stimuli]) × 100] to compare the magnitude of the DEX effects on CPM.ResultsThe inhibition rates of ampSEPs and VASt in Types N, L and H varied significantly, demonstrating a dose‐dependent reduction of CPM effects of ampSEP and VASt during randomized DEX administration, consistent with results from animal studies.ConclusionsThe present study shows that systemic administration of an α2‐adrenoceptor agonist (DEX), less than the clinical dose, inhibited CPM in humans. These results may provide some mechanistic insight into why many chronic pain patients show impaired CPM.
Reclaiming Literacy Classrooms Through Critical Dialogue
Authors: Fecho Bob , Coombs Dawan , McAuley Sean 
Published: March 2012

AbstractAuthors Fecho, Coombs, and McAuley discuss the integral role of dialogue in literacy classrooms dominated by standardized testing, curriculum, and instruction. Their argument in support of the dialogical literacy classroom begins with a historical and theoretical justification for these principles, then transitions into a discussion of the troubling condition of schools as a result of the current lack of dialogue. By including the voices of students and teachers, the authors offer glimpses into classrooms in need of dialogical transactions. This commentary concludes with a call to teachers, policy makers, and community members to consider what educators risk when they fail to open up classrooms as places where diverse perspectives can be heard and individual meaning can be made of the texts of students’ lives. In addition, the authors argue here that establishing a critical dialogue in literacy classrooms is needed, perhaps now more than at any time in our past.
“Without Comic Books, There Would Be No Me”: Teachers as Connoisseurs of Adolescents’ Literate Lives
Authors: Simon Rob 
Published: March 2012

AbstractThis article explores the notion of connoisseurship as a framework for learning about adolescents’ lives and literacies and developing relationships in literacy classrooms. Drawing upon data from a two‐year qualitative study of the collaborative inquiries of a community of student English teachers, the author examines inquiry projects written for an English methods course in which two student teachers, Alex and Jared, construct counternarratives about the promise of students who had been positioned as problems within their schools. These examples illustrate how becoming connoisseurs of adolescents’ distinctiveness and abilities can be a basis for developing counterpractices in literacy classrooms, opening spaces of possibility for otherwise struggling students to begin to reorient their negative trajectories in school. Among other implications, this work suggests the positive consequences of teachers’ attentiveness to students’ literacy practices, and the importance of teachers cultivating more relational stances toward students.
The Power of the Sketch(Book): Reflections From High School English Students
Authors: Rebecca Leigh S. 
Published: March 2012

AbstractThis paper explores the potential of sketchbooks as a pathway for developing ideas for writing in high school English classrooms. This paper examines how shifting between drawing and writing impacts students’ ability to develop ideas for writing and create meaningful texts. Specifically, this paper explores four types of visual sign making that students use in mediating, expressing, and representing meaning. Findings in this study suggest that sketchbooks can be used as an effective tool for inquiry in the pedagogy of writing instruction. As well, students’ voices offer clarification on perceptions about art and language as modes of expression which suggest curricular changes for the secondary English classroom.
The political economy of financial reform: How robust are huang's findings?
Authors: Zandberg Eelco , Haan Jakob De , Paul Elhorst J. 
Published: March 2012

SUMMARYUsing new data on financial liberalization taken from Abiad et al. (2008, IMF Working Papers, No. 08/266) for 62 countries over the period 1975–2005, we show that some of the main findings of Huang's (2009, Journal of Applied Econometrics 24: 1207–1213) replication study on the drivers of financial liberalization are not robust. Major changes concern the effect of democracy on financial reform and the supposed relationship between the desired level of financial liberalization and a country's per capita income. Copyright © 2012 John Wiley & Sons, Ltd.
The impact of immobilization of BMP‐2 on PDO membrane for bone regeneration
Authors: Kim Ji‐Eun , Lee Eun‐Jung , Kim Hyoun‐Ee , Koh Young‐Hag , Jang Jun‐Hyeog 
Published: March 2012

AbstractPoly(dioxanone) (PDO) is colorless, crystalline, a biodegradable synthetic polymers that is used for biomedical applications, such as surgical sutures, cardiovascular applications, orthopedics, and plastic surgery. Recently, bone morphogenetic protein‐2 (BMP‐2) is widely used for bone tissue engineering. For the first time we report here on the in vitro performance of an electrospun PDO membrane immobilized with BMP‐2. Immobilized BMP‐2 on PDO membrane enhanced ALPase activity, the osteogenic differentiation gene expressions as well as cell attachment, except cell proliferation when compared to that of PDO membrane alone. These results suggest that PDO membrane with BMP‐2 is helpful to promote bone healing and regeneration. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
Chemical reactivity in the framework of pair density functional theories
Authors: Otero Nicolás , Mandado Marcos 
Published: March 2012

AbstractChemical reactivity descriptors are derived within the framework of the pair density functional theory. These indices provide valuable information about bonding rearrangements and activating mechanisms upon electrophilic or nucleophilic reactions. Indices derived and tested in this work represent nonlocal counterparts of the local reactivity indices derived in the context of conceptual density functional theory (CDFT) and frequently used in reactivity studies; the Fukui function, the local softness and the dual descriptor. In this work, we show how these nonlocal indices provide a quantum chemical basis to explain the success of qualitative resonance models in chemical reactivity predictions. Also, local information is implicitly contained as CDFT indices are obtained by simple integration. As illustrative examples, we have considered in this work the Markovnikov's rule, the reactivity of enolate anion, the nucleophilic conjugate addition to α,β‐unsaturated compounds and the electrophilic aromatic substitution of benzene derivatives. The densities used in this work were obtained with Hartree‐Fock, Kohn‐Sham DFT, and singles and doubles configuration interaction (CISD) approaches. © 2012 Wiley Periodicals, Inc.
Electrochemical Behaviours and Detection of Adenosine‐5¢‐triphosphate on an Ionic Liquid Modified Carbon Paste Electrode
Authors: Niu Qing‐juan , Liu Jun , Li Guang‐jiu , Qin Hong‐qing , Gao Hong‐wei , Sun Wei 
Published: March 2012

AbstractIn this paper a new electrochemical method based on the ionic liquid modified carbon paste electrode (IL‐CPE) was proposed for the determination of adenosine‐5′‐triphosphate (ATP) in a pH 4.5 Britton‐Robinson (B‐R) buffer solution. IL‐CPE was prepared by using 1‐butyl‐3‐methylimidazolium trifluoroacetate (BMIMCF3COO) as the modifier. Cyclic voltammetry was used to investigate the electrochemical behaviors of ATP on the IL‐CPE, and the results indicated that IL‐CPE exhibited strong electrocatalytic ability to promote the oxidation of ATP with a single well‐defined irreversible adsorption‐controlled oxidation peak appeared. The electrochemical reaction parameters of ATP were calculated with the results of the electron transfer coefficient (α) as 0.40, the electron transfer number (n) as 1.17, the apparent heterogeneous electron transfer rate constant (ks) as 3.66 × 10‐6 s‐1 and the surface coverage (Γτ) as 2.48 × 10‐9 mol cm‐2. Under the selected conditions the proposed IL‐CPE showed good performances to the ATP detection in the concentration range from 0.1 to 1000.0 μmol L‐1 with the detection limit as 3.65 × 10‐8 mol L‐1 (3σ) by differential pulse voltammetry. The method showed good selectivity to the ATP detection without the interferences of coexisting substances and was successfully applied to the ATP injection samples detection with satisfactory results.
Microwave Promoted, Sodium Acetate Catalyzed One Pot Synthesis of Poly Functionalized 4H‐Pyrans
Authors: Pandharpatte Mahesh Shivaji , Mulani Khudbudin Baban , Mohammed Nazeruddin Nasiruddin Gulam 
Published: March 2012

AbstractA clean and efficient one pot synthesis of 4H‐pyran derivatives through condensation of aromatic aldehydes, malononitrile and dicarbonyl compounds under microwave irradiation in presence of Sodium acetate as a catalyst is described.
Structure and Dielectric Properties of NiTiO3 Powders Synthesized by the Modified Sol–Gel Method
Authors: Chuang Shiow‐Huey , Hsieh Min‐Lung , Wang Ding‐Yeong 
Published: March 2012

AbstractThis study reports the synthesis of nickel titanate (NiTiO3) powders by using the modified sol‐gel method, with nickel acetate tetrahydrate as the nickel source, titanium isopropoxide as the titanium source, and 2‐methoxyethanol as the solvent, followed by post‐heat treatment in air at temperatures ranging from 500 °C to 900 °C. The characteristics of powders were determined by X‐ray diffraction (XRD), FT‐infrared spectroscopy (FT‐IR), ultraviolet/visible spectroscopy (UV/Vis), and Raman spectroscopy. The particle size and surface area of the powders were also measured. The results indicated that single‐phase NiTiO3 can be prepared using the modified sol‐gel method, followed by post‐heat treatment at the relatively low temperature of 550 °C. The crystallite sizes and particle sizes of NiTiO3 powders increase in conjunction with the post‐heat treatment temperatures. However, the surface area of the powders shrinks as the post‐heat treatment temperatures increase. The dielectric constants of NiTiO3 powders, based on the capacitance‐voltage analysis, are within a range of 13.2 to 17.8.
Low density lipoprotein receptor‐related protein 1 modulates the proliferation and migration of human hepatic stellate cells
Authors: Llorente‐Cortes V. , Barbarigo V. , Badimon L. 
Published: March 2012

AbstractHuman hepatic stellate cells (HHSCs) proliferation and migration play a key role in the pathogenesis of liver inflammation and fibrogenesis. Low density lipoprotein receptor‐related protein (LRP1) is an endocytic receptor involved in intracellular signal transduction. The aim of this work was to analyse the role of low density lipoprotein receptor‐related protein (LRP1) in HHSCs proliferation and migration and the mechanisms involved. Human LRP1 deficient‐HHSCs were generated by nucleofecting the line HHSCs with siRNA anti‐LRP1. HHSCs DNA synthesis was measured by [3H]‐thymidine incorporation and cell cycle progression by flow cytometry after annexin V and iodure propidium staining. Cell migration was assessed using a wound repair model system. LRP1 expression and extracellular matrix‐regulated kinase (ERK1,2) phosphorylation were analysed by Western blot analysis. Transforming growth factor‐β (TGF‐β) extracellular levels were analysed by ELISA. siRNA‐antiLRP1 treatment almost completely inhibited LRP1 mRNA and protein expression. LRP1 deficient HHSCs showed higher proliferative response (172 ± 19 vs. 93 ± 8 [3H]‐thymidine incorporation; 78.68% vs. 82.69% in G0/G1, 21.32% vs. 17.30% in G2/S) and higher migration rates than control HHSCs. LRP1 deficient cells showed higher levels of phosphorylated ERK1,2. TGF‐β extracellular levels were threefold higher in LRP1‐deficient than in control HHSCs cells. These results demonstrate that LRP1 regulates HHSCs proliferation and migration through modulation of ERK1,2 phosphorylation and TGF‐β extracellular levels. These results suggest that HHSCs‐LRP1 may play a key role in the modulation of factors determining hepatic fibrosis. J. Cell. Physiol. 227: 3528–3533, 2012. © 2012 Wiley Periodicals, Inc.
Protein sequences involved in the mitochondrial import of the 3,5,3′‐L‐triiodothyronine receptor p43
Authors: Carazo Angel , Levin Jonathan , Casas François , Seyer Pascal , Grandemange Stéphanie , Busson Muriel , Pessemesse Laurence , Wrutniak‐Cabello Chantal , Cabello Gérard 
Published: March 2012

AbstractThe major effect of T3 on mitochondrial activity has been partly explained by the discovery of p43, a T3‐dependent transcription factor of the mitochondrial genome. P43 is imported into mitochondria in an atypical manner which is not yet fully understood. Our aim was to characterize the p43 sequences inducing its mitochondrial import, using in organello import experiments with wild‐type or mutated proteins and validation in CV1 cells. We find that several sequences define the mitochondrial addressing. Two alpha helices in the C‐terminal part of p43 are actual mitochondrial import sequences as fusion to a cytosolic protein induces its mitochondrial translocation. Helix 5 drives the atypical mitochondrial import process, whereas helices 10/11 induce a classical import process. However, despite its inability to drive a mitochondrial import, the N‐terminal region of p43 also plays a permissive role as in the presence of the C‐terminal import sequences different N‐terminal regions determine whether the protein is imported or not. These results can be extrapolated to other mitochondrial proteins related to the nuclear receptor superfamily, devoid of classical mitochondrial import sequences. J. Cell. Physiol. 227: 3768–3777, 2012. © 2012 Wiley Periodicals, Inc.
Establishment of keratinocyte colonies from small‐sized cervical intraepithelial neoplasia specimens
Authors: Bononi Ilaria , Bosi Silvia , Bonaccorsi Gloria , Marci Roberto , Patella Alfredo , Ferretti Stefano , Tognon Mauro , Garutti Paola , Martini Fernanda 
Published: March 2012

AbstractThe size of human cervical intraepithelial neoplasia (CIN) biopsies is usually very small and standard methods do not allow an adequate number of keratinocytes to be isolated for culturing purposes. In this study, a new approach to establish keratinocyte cultures from small CIN a tissue fragments was developed. Neoplastic specimens and corresponding normal tissues, which were used as controls, were digested with collagenase. Tissue‐derived fibroblasts and keratinocytes were co‐cultured in calcium and serum medium. Single keratinocyte colonies from primary cultures were expanded using a culture medium optimized in our laboratory. Primary keratinocyte colonies, as well as expanded colonies, were tested for epithelial and cervical markers such as 5, 14, 17, and 19 keratins, and p63 by immunofluorescence. Our results indicate that a variable number of primary keratinocyte colonies could be detected in neoplastic cultures, depending on the grade of cervical lesions from which the colonies originated. Single colonies, when cultured with our new medium, grew at a high rate with uniform size and morphology for some passages. Epithelial and p63 markers were expressed in keratinocyte colonies, as well as in expanded colonies. In conclusion, our study reports a rapid and easy culturing system which enables keratinocyte colonies from minute cervical tumor tissues to be obtained. Moreover, using the new culture medium, keratinocyte colonies can be expanded at a high proliferative rate. J. Cell. Physiol. 227: 3787–3795, 2012. © 2012 Wiley Periodicals, Inc.
Micro‐ and nanostructures of iridescent wing scales in purple emperor butterflies (Lepidoptera: Apatura ilia and A. iris)
Authors: Ćurčić Srećko B. , Pantelić Dejan V. , Ćurčić Božidar P.M. , Savić‐Šević Svetlana N. , Makarov Slobodan E. , Lačković Vesna B. , Labudović‐Borović Milica M. , Ćurčić Nina B. , Stojanović Dejan V. 
Published: March 2012

AbstractApatura ilia (Denis and Schiffermüller, 1775) and A. iris (Linnaeus, 1758) are fascinating butterflies found in the Palaearctic ecozone (excepting the north of Africa). The wings of these insects are covered with a great number of two types of scales positioned like roof tiles. Type I scales are on the surface, while type II scales are situated below them. The structural color of the type I scales is recognized only on the dorsal side of both the fore and hind wings of the males of the aforementioned species. Both types of scales are responsible for pigment color of the wings, but iridescence is observed only in the type I scales. The brilliant structural color is due to a multilayer structure. The features of the scales, their dimensions and fine structure were obtained using scanning electron microscopy. Cross sections of the scales were then analyzed by transmission electron microscopy. The scales of the “normal” and clytie forms of A. ilia have a different nanostructure, but are of the same type. A similar type of structure, but with a different morphology, was also noticed in A. iris. The scales of the analyzed species resemble the scales of tropical Morpho butterflies. Microsc. Res. Tech., 2012. © 2012 Wiley Periodicals, Inc.
Correlation of perfusion parameters on dynamic contrast‐enhanced MRI with prognostic factors and subtypes of breast cancers
Authors: Koo Hye Ryoung , Cho Nariya , Song In Chan , Kim Hyeonjin , Chang Jung Min , Yi Ann , Yun Bo La , Moon Woo Kyung 
Published: March 2012

AbstractPurpose:To investigate whether a correlation exists between perfusion parameters obtained from dynamic contrast‐enhanced (DCE) magnetic resonance imaging (MRI) and prognostic factors or immunohistochemical subtypes of breast cancers.Materials and Methods:Quantitative parameters (Ktrans, kep, and ve) of 70 invasive ductal carcinomas were obtained using DCE‐MRI as a postprocessing procedure. Correlations between parameters and prognostic factors, including tumor size, axillary nodal status, histologic grade, nuclear grade, expression of estrogen receptor (ER), progesterone receptor (PR), Ki‐67, p53, bcl‐2, and human epidermal growth factor receptor 2 (HER2) and subtypes categorized as luminal (ER or PR‐positive), triple negative (ER or PR‐negative, HER2‐negative), and HER2 (ER and PR‐negative, HER2 overexpression) were analyzed.Results:Mean Ktrans was higher in tumors with a high histologic grade than with a low histologic grade (P = 0.007), with a high nuclear grade than with a low nuclear grade (P = 0.002), and with ER negativity than ER positivity (P = 0.056). Mean kep was higher in tumors with a high histologic grade than with a low histologic grade (P = 0.005), with a high nuclear grade than with a low nuclear grade (P = 0.001), and with ER negativity than with ER positivity (P = 0.043). Mean ve was lower in tumors with a high histologic grade than with a low histologic grade (P = 0.038) and with ER negativity than with ER positivity (P = 0.015). Triple‐negative cancers showed a higher mean kep than the luminal type (P = 0.015).Conclusion:Breast cancers with higher Ktrans and kep, or lower ve, had poor prognostic factors and were often of the triple‐negative subtype. J. Magn. Reson. Imaging 2012;36:145–151. © 2012 Wiley Periodicals, Inc.
The diagnostic value of nipple discharge cytology: Breast imaging complements predictive value of nipple discharge cytology
Authors: Kalu Ogori N. , Chow Cassandra , Wheeler Amanda , Kong Christina , Wapnir Irene 
Published: March 2012

AbstractBackgroundPapilloma is the most common finding associated with pathologic nipple discharge. In the absence of breast imaging abnormalities, the incidence of occult malignancy is <3%.ObjectiveTo determine the predictive value of nipple discharge cytology in conjunction with breast imaging.MethodsRetrospective review of 160 charts; inclusion criteria of clinically pathologic nipple discharge, subsequent excisional biopsy, and absence of palpable abnormalities. Nipple discharge cytology categorized as negative, atypical, suspicious, and papillary. Breast imaging was analyzed. Preoperative tests were correlated to final surgical pathology.Results89 patients identified. Sixty‐five had positive cytology, with a false positive rate of 32.3%. They were associated with papillomas in 52%, benign non‐papillary in 33% and malignant lesions in 9% of cases. Nipple discharge cytology was positive in 69.6% of papillomas and 92% of atypical/malignant lesions; 30% had abnormal breast imaging and positive cytology. Nipple discharge cytology had a sensitivity of 74.5%, specificity of 30%, and positive predictive value of 68%. The positive predictive value increased to 85% with associated abnormal breast imaging.ConclusionsNipple discharge cytology is useful in evaluating pathologic discharge. However, negative cytology with negative imaging is not enough to avoid surgery in cases of suspicious clinical presentation. J. Surg. Oncol. 2012; 106:381–385. © 2012 Wiley Periodicals, Inc.