bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel polymer‐free paclitaxel‐eluting stent with a nanoporous surface for rapid endothelialization and inhibition of intimal hyperplasia: Comparison with a polymer‐based sirolimus‐eluting stent and bare metal stent in a porcine model
Authors: Jia Haibo , Liu Haixia , Kong Junying , Hou Jingbo , Wu Jian , Zhang Maomao , Tian Jinwei , Liu Huimin , Ma Lijia , Hu Sining , Huang Xingtao , Zhang Shaosong , Zhang Shuo , Yu Bo , Jang Ik‐Kyung 
Published: July 2011

AbstractHypersensitivity and inflammatory responses to polymers may be responsible for late stent thrombosis after implantation of a drug‐eluting stent (DES). Polymer‐free DES may reduce the prevalence of these adverse reactions in vessels. We evaluated a polymer‐free paclitaxel‐eluting‐stent with a nanoporous surface (nano‐PES) for endothelialization and inhibition of neointimal hyperplasia by optical coherence tomography (OCT) and pathology in a porcine model. Nano‐PES with high‐dose (HD) and low‐dose (LD) paclitaxel (1.0 μg/mm2 and 0.4 μg/mm2, respectively) was compared with a sirolimus‐eluting stent (SES) and bare‐metal stent (BMS) in a porcine model. Fifty‐three stents (14 HD, 14 LD, 14 SES, 11 BMS) were implanted in 18 minipigs. At 14 days, nano‐PES with HD and LD showed more complete endothelialization compared with SES. BMS had 100% endothelial coverage. At 28 days, a significant reduction in neointimal hyperplasia was detected by OCT in the nano‐PES HD group compared with BMS. No benefit in prevention of the neointimal hyperplasia was observed in the nano‐PES LD group. Nano‐PES stents showed decreased deposition of fibrin and inflammation compared with SES. Pharmacokinetic studies revealed that nano‐PES could effectively deliver the drug to the local coronary artery and it released the drug more rapidly than SES. Such a release profile was favorable for rapid endothelialization of nano‐PES. The present study showed the nano‐PES to be a new drug‐delivery technology; that it used a nanoporous stent surface; that it offered desirable drug‐elution properties without the use of polymers; that it may translate into an improved safety profile for next‐generation DES. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
Agreement between self‐report and archival public service utilization data among chronically homeless individuals with severe alcohol problems
Authors: Clifasefi Seema L. , Collins Susan E. , Tanzer Kenneth , Burlingham Bonnie , Hoang Sara E. , Larimer Mary E. 
Published: July 2011

AbstractPublic service utilization data are often used as key outcomes in studies on homelessness. Although self‐report data on these outcomes are accessible and cost‐effective, various factors may affect retrospective recall in homeless populations. It is therefore necessary to establish validity of self‐report to ensure the integrity of studies involving such populations. Participants (N=134) were chronically homeless individuals with severe alcohol problems who participated in a housing first effectiveness trial (Larimer et al., 2009). The authors compared 30‐day and 3‐year retrospective self‐report data on sobering center, jail, and hospital use with archival records corresponding to the same timeframes. Analyses indicated good category‐specific agreement for 30‐day self‐report and archival data on sobering center (82%; κ=.58) and jail use (89%; κ=.60). Hospital use, however, was self‐reported significantly more frequently than indicated by archival data (78%; κ=.30). Three‐year data showed inadequate agreement across all three variables. © 2011 Wiley Periodicals, Inc.
Sense of community and interethnic relations: comparing local communities varying in ethnic heterogeneity
Authors: Castellini Federica , Colombo Monica , Maffeis Daniele , Montali Lorenzo 
Published: July 2011

AbstractThis study investigates the sense of community and interethnic relations in two different metropolitan areas that vary in ethnic heterogeneity. The study was conducted in Milan, Italy using a sample of 318 participants living in different city districts that vary in ethnic heterogeneity (low vs. high). The participants completed a questionnaire that assessed their psychological sense of community, blatant and subtle prejudice, host community acculturation strategies, and some measures of contact (quality, quantity, and type). The results indicate that high ethnic heterogeneity is related to a lower sense of community, higher blatant prejudice, and exclusionism. Residents living in lower ethnic heterogeneity neighborhoods reported closer contact opportunities and lower levels of prejudice. © 2011 Wiley Periodicals, Inc.
Zearalenone induces apoptosis and necrosis in porcine granulosa cells via a caspase‐3‐ and caspase‐9‐dependent mitochondrial signaling pathway
Authors: Zhu Li , Yuan Hui , Guo Chengzhi , Lu Yin , Deng Sijun , Yang Yang , Wei Qiang , Wen Lixin , He Zuping 
Published: July 2011

AbstractZearalenone is a mycotoxin produced mainly by Fusarium. There are numerous incidences of mycotoxicosis in laboratory and domestic animals, especially in pigs. However, little is known about molecular mechanisms of zearalenone toxicity. Granulosa cells are the maximal cell population in follicles, and they play an essential role in the development and maturation of follicles. The objective of this study was to explore the effect of zearalenone at high concentrations on proliferation and apoptosis of porcine granulosa cells and uncover signaling pathway underlying the cytotoxicity of zearalenone. We found that zearalenone reduced the proliferation of porcine granulosa cells in a dose‐dependent manner as shown by the MTT assay and zearalenone resulted in an obvious apoptosis and necrosis in porcine granulosa cells as determined by the TUNEL analysis and flow cytometry. In addition, TUNEL assay with caspase inhibitors showed that zearalenone triggered a caspase‐3‐ and caspase‐9‐dependent apoptotic process in porcine granulosa cells. Fluorescence spectrophotometer displayed that zearalenone led to a loss of mitochondrial transmembrane potential of porcine granulosa cells but enhanced reactive oxygen species (ROS) levels of the cells. Notably, Western blots revealed that caspase‐3 and caspase‐9 were activated by zearalenone in porcine granulosa cells. Collectively, our results suggest that zearalenone induces apoptosis and necrosis of porcine granulosa cells in a dose‐dependent manner via a caspase‐3‐ and caspase‐9‐dependent mitochondrial pathway. This study thus offers a novel insight into molecular mechanisms by which zearalenone has adverse cytotoxicity on reproduction. J. Cell. Physiol. 227: 1814–1820, 2012. © 2011 Wiley Periodicals, Inc.
Mouse embryonic stem cells that survive γ‐rays exposure maintain pluripotent differentiation potential and genome stability
Authors: Rebuzzini Paola , Pignalosa Diana , Mazzini Giuliano , Di Liberto Riccardo , Coppola Antonio , Terranova Nadia , Magni Paolo , Redi Carlo Alberto , Zuccotti Maurizio , Garagna Silvia 
Published: July 2011

AbstractThis study aimed to investigate the cell cycle, apoptosis, cytogenetics and differentiation capacity of mouse embryonic stem cells (mESCs) that survived a single dose of 2 or 5 Gy γ‐rays during a period of up to 96 h of culture. After 2 Gy irradiation and 24 h culture, compared to control, a significant majority of cells was blocked at the G2/M phase and a massive apoptosis was recorded. Between 48 and 72 h post‐irradiation, the parameters used to describe the cell cycle and apoptosis returned similar to those of control samples. When mESCs were irradiated with 5 Gy, a small fraction of cells, even after 96 h of culture, still presented clear evidences of a G2/M block and apoptosis. The cytogenetic analysis performed at 96 h showed that the structural stability of the aberrations did not change significantly when comparing control and 2 or 5 Gy‐treated populations. However, the chromosomal damage observed in the progeny of the survived cells after 5 Gy exposure is significantly higher than that observed in control samples, although it is mostly of the stable and transmissible type. Ninety‐six hours after irradiation, the survived mESCs maintained their undifferentiated status and capability to differentiate into the three germ layers. Overall, these results indicate a commitment of mESCs to maintain pluripotency and genome stability. J. Cell. Physiol. 227: 1242–1249, 2012. © 2011 Wiley Periodicals, Inc.
Afadin controls p120‐catenin–ZO‐1 interactions leading to endothelial barrier enhancement by oxidized phospholipids
Authors: Birukova Anna A. , Fu Panfeng , Wu Tinghuai , Dubrovskyi Oleksii , Sarich Nicolene , Poroyko Valery , Birukov Konstantin G. 
Published: July 2011

AbstractAfadin is a novel regulator of epithelial cell junctions assembly. However, its role in the formation of endothelial cell junctions and the regulation of vascular permeability remains obscure. We previously described protective effects of oxidized 1‐palmitoyl‐2‐arachidonoyl‐sn‐glycero‐3‐phosphorylcholine (OxPAPC) in the in vitro and in vivo models of lung endothelial barrier dysfunction and acute lung injury, which were mediated by Rac GTPase. This study examined a role of afadin in the OxPAPC‐induced enhancement of interactions between adherens junctions and tight junctions as a novel mechanism of endothelial cell (EC) barrier preservation. OxPAPC induced Rap1‐dependent afadin accumulation at the cell periphery and Rap1‐dependent afadin interaction with adherens junction and tight junction proteins p120‐catenin and ZO‐1, respectively. Afadin knockdown using siRNA or ectopic expression of afadin mutant lacking Rap1 GTPase binding domain suppressed OxPAPC‐induced EC barrier enhancement and abolished barrier protective effects of OxPAPC against thrombin‐induced EC permeability. Afadin knockdown also abolished protective effects of OxPAPC against ventilator‐induced lung injury in vivo. These results demonstrate for the first time a critical role of afadin in the regulation of vascular barrier function in vitro and in vivo via coordination of adherens junction–tight junction interactions. J. Cell. Physiol. 227: 1883–1890, 2012. © 2011 Wiley Periodicals, Inc.
Hyal‐1 but not hyal‐3 deficiency has an impact on ovarian folliculogenesis and female fertility by altering the follistatin/activin/Smad3 pathway and the apoptotic process
Authors: Dumaresq‐Doiron Karine , Edjekouane Lydia , Orimoto Adriana Mari , Yoffou Paule Héléna , Gushulak Lara , Triggs‐Raine Barbara , Carmona Euridice 
Published: July 2011

AbstractOvarian follicle development is a process regulated by various endocrine, paracrine and autocrine factors that act coordinately to promote follicle growth. However, the vast majority of follicles does not reach the pre‐ovulatory stage but instead, undergo atresia by apoptosis. We have recently described a role for the somatic hyaluronidases (Hyal‐1, Hyal‐2, and Hyal‐3) in ovarian follicular atresia and induction of granulosa cell apoptosis. Herein, we show that Hyal‐1 but not Hyal‐3 null mice have decreased apoptotic granulosa cells after the induction of atresia and an increased number of retrieved oocytes after stimulation of ovulation. Furthermore, young Hyal‐1 null mice had a significantly higher number of primordial follicles than age matched wild‐type animals. Recruitment of these follicles at puberty resulted in an increased number of primary and healthy preantral follicles in Hyal‐1 null mice. Consequently, older Hyal‐1 deficient female mice have prolonged fertility. At the molecular level, immature Hyal‐1 null mice have decreased mRNA expression of follistatin and higher levels of phospho‐Smad3 protein, resulting in increased levels of phospho‐Akt in pubertal mice. Hyal‐1 null ovarian follicles did not exhibit hyaluronan accumulation. For Hyal‐3 null mice, compensation by Hyal‐1 or Hyal‐2 might be related to the lack of an ovarian phenotype. In conclusion, our results demonstrate that Hyal‐1 plays a key role in the early phases of folliculogenesis by negatively regulating ovarian follicle growth and survival. Our findings add Hyal‐1 as an ovarian regulator factor for follicle development, showing for the first time an interrelationship between this enzyme and the follistatin/activin/Smad3 pathway. J. Cell. Physiol. 227: 1911–1922, 2012. © 2011 Wiley Periodicals, Inc.
NADPH oxidase 4 mediates upregulation of type 4 phosphodiesterases in human endothelial cells
Authors: Muzaffar Saima , Jeremy Jamie Y. , Angelini Gianni D. , Shukla Nilima 
Published: July 2011

AbstractThe protective actions of prostacyclin (PGI2) are mediated by cyclic AMP (cAMP) which is reduced by type 4 phosphodiesterases (PDE4) which hydrolyze cAMP. Superoxide ${\left( {{\rm O}_{2}^{{-} } } \right)}$ from NADPH oxidase (Nox) is associated with impaired PGI2 bioactivity. The objective of this study, therefore, was to study the relationship between Nox and PDE4 expression in human umbilical vein endothelial cells (HUVECs). HUVECs were incubated with the thromboxane A2 analog, U46619, 8‐isoprostane F2α (8IP), or tumor necrosing factor alpha (TNFα) [±iloprost (a PGI2 analog)] and the expression of PDE4A, B, C, and D and splice variants thereof assessed using Western blotting and qPCR and mRNA silencing of Nox4 and Nox5. Effects on cell replication and angiogenesis were also studied. U46619, 8IP, and TNFα increased the expression of Nox 4 and Nox 5 and all PDE4 isoforms as well as cell replication and tubule formation (index of angiogenesis), effects inhibited by mRNA silencing of Nox4 (but not Nox5) and iloprost and rolipram. These data demonstrate that upregulation of Nox4 leads to an upregulation of PDE4A, B, and D and increased hydrolysis of cAMP which in turn augments cell replication and angiogenesis. This mechanism may be central to vasculopathies associated with endothelial dysfunction since the PGI2–cAMP signaling axis plays a key role in mediating functions that include hemostasis and angiogenesis. J. Cell. Physiol. 227: 1941–1950, 2012. © 2011 Wiley Periodicals, Inc.
Rspo2/Int7 regulates invasiveness and tumorigenic properties of mammary epithelial cells
Authors: Klauzinska Malgorzata , Baljinnyam Bolormaa , Raafat Ahmed , Rodriguez‐Canales Jaime , Strizzi Luigi , Endo Greer Yoshimi , Rubin Jeffrey S. , Callahan Robert 
Published: July 2011

AbstractRspo2 was identified as a novel common integration site (CIS) for the mouse mammary tumor virus (MMTV) in viral induced mouse mammary tumors. Here we show that Rspo2 modulates Wnt signaling in mouse mammary epithelial cells. Co‐expression of both genes resulted in an intermediate growth phenotype on plastic and had minor effects on the growth‐promoting properties of Wnt1 in soft agar. However, individual Rspo2 and Wnt1 HC11 transfectants as well as the double transfectant were tumorigenic in athymic nude mice, with tumors from each line having distinctive histological characteristics. Rspo2 and Rspo2/Wnt1 tumors contained many spindle cells, consistent with an epithelial–mesenchymal transformation (EMT) phenotype. When Rspo2 and Rspo2/Wnt1 tumor cells were transferred into naïve mice, they exhibited greater metastatic activity than cells derived from Wnt1 tumors. For comparison, C57MG/Wnt1/Rspo2 co‐transfectants exhibited invasive properties in three‐dimensional (3D) Matrigel cultures that were not seen with cells transfected only with Wnt1 or Rspo2. Use of Dickkopf‐1, a specific antagonist of the Wnt/β‐catenin pathway, or short hairpin RNA targeting β‐catenin expression demonstrated that the invasive activity was not mediated by β‐catenin. Our results indicate that Rspo2 and Wnt1 have mutually distinct effects on mammary epithelial cell growth and these effects are context‐dependent. While Rspo2 and Wnt1 act synergistically in the β‐catenin pathway, other mechanisms are responsible for the invasive properties of stable double transfectants observed in 3D Matrigel cultures. J. Cell. Physiol. 227: 1960–1971, 2012. © 2011 Wiley Periodicals, Inc.
3H‐L‐leucine transport by the promiscuous crustacean dipeptide‐like cotransporter
Authors: Obi I. , Wells A.L. , Ortega P. , Patel D. , Farah L. , Zanotto F.P. , Ahearn G.A. 
Published: July 2011

AbstractThe crustacean intestine and hepatopancreas display a variety of solute transport mechanisms for transmembrane transfer of dietary contents from lumen to epithelial cytosol. An in vitro intestinal perfusion apparatus was used to characterize mucosal to serosoal (MS) and serosal to mucosal (SM) Zn2+‐dependent 3H‐L‐leucine transport by the intestine of the American lobster, Homarus americanus. Transmural 20 µM MS 3H‐L‐leucine fluxes across lobster intestine were a hyperbolic function of luminal zinc concentration (1–50 µM) following Michaelis–Menten kinetics (Km = 2.67 ± 0.74 µM; Jmax = 19.56 ± 2.22 pmol/cm2×min). Transmural 20 µM SM 3H‐L‐leucine fluxes were not affected by serosal zinc, resulting in a highly significant stimulation of net amino acid transfer to the blood by luminal metal. MS fluxes of 20 µM 3H‐L‐leucine were also hyperbolic functions of luminal [Cu2+], [Mn2+], [Na+], and [H+]. MS flux of 3H‐L‐leucine was a sigmoidal function of luminal [L‐leucine] and was stimulated by the addition of 20 µM luminal zinc at both pH 7.0 and 5.5. A greater enhanced amino acid transport occurred at the lower pH 5.5. MS flux of 20 µM 3H‐L‐leucine in the presence of 20 µM zinc was significantly inhibited by addition of 100 µM luminal glycylsarcosine, and MS flux of 20 µM 3H‐glycylsarcosine was inhibited by 100 µM L‐leucine in the presence of 20 µM zinc. Results suggest that 3H‐L‐leucine and metals form a complex (e.g., Leu–Zn–Leu] that may functionally mimic dipeptides and use a dipeptide‐like transporter during MS fluxes as suggested for fish and mammals. J. Exp. Zool. 315:465–475, 2011. © 2011 Wiley‐Liss, Inc.
Prenatal immune challenge compromises the normal course of neurogenesis during development of the mouse cerebral cortex
Authors: Soumiya Hitomi , Fukumitsu Hidefumi , Furukawa Shoei 
Published: July 2011

AbstractMaternal infection during pregnancy is an environmental risk factor for the development of severe brain disorders in offspring, including schizophrenia and autism. However, little is known about the neurodevelopmental mechanisms underlying the association between prenatal exposure to infection and the emergence of cognitive and behavioral dysfunctions in later life. By injecting viral mimetic polyriboinosinic‐polyribocytidylic acid (Poly I:C) into mice, we investigated the influence of maternal immune challenge during pregnancy on the development of the cerebral cortex of offspring. Our previous study showed that stimulation of the maternal immune system compromised the expression properties of transcription factors and the synaptogenesis of cortical neurons in upper layers but not those in deeper layers. The objective of the current study was to examine further whether maternal immune challenge has an influence on the cellular–biological features of the cortical progenitors that generate distinct cortical neuronal subtypes. We found the following abnormalities in the cortex of mice given the prenatal Poly I:C injection during later stages of cortical neurogenesis. First, proliferative activity and the expression of Pax6, which is a master regulator of the gene expression of transcription factors, were significantly decreased in the cortical progenitors. Second, the laminar allocation and gene expression were significantly altered in the daughter neurons generated at the same birth dates. These results demonstrate that specific abnormalities in the cortical progenitors preceded deficits in neuronal phenotypes. These changes may underlie the emergence of psychiatric brain and behavioral dysfunctions after in utero exposure to an infection. © 2011 Wiley‐Liss, Inc.
Changes in phosphorus fractions in the rhizosphere of some crop species under glasshouse conditions
Authors: Safari Sinegani Ali Akbar , Rashidi Tahere 
Published: July 2011

AbstractThe aim of the present study was to evaluate the effect of cultivation of some agronomic plant species on inorganic soil‐P fractions in different sampling zones. The experiment was conducted as a completely randomized design as factorial in three replicates. The factors were plant species (10 different species and control) and soil‐sampling zone (soil adhering to root mat, rhizosphere soil, and nonrhizospheric soil). The thin‐slicing technique was modified and used to sample rhizosphere soil. The percentages of P fractions in the planted and control soils were near 64% apatite (apatite‐P), 24% octa‐Ca‐phosphates (OCP‐P), 7% Fe‐phosphates (Fe‐P), 4% di‐Ca‐phosphates (DCP‐P), 1% Al‐phosphates (Al‐P), and 0% occluded‐P (O‐P). All of the studied plant species decreased significantly all of the inorganic P fractions in soil adhering to root mat and in rhizosphere soil compared to those in nonrhizosphere soil. However, these decreases were not equal for each fraction and the percentage of apatite‐P increased in rhizosphere soil of the plant species. The means of total P, soluble P, DCP‐P, OCP‐P, Al‐P, and Fe‐P were lower in soil adhering to root mat compared to those in rhizosphere soil. However, this difference was only significant for OCP‐P. In contrast, the mean of apatite‐P in soil adhering to root mat was significantly higher than that in rhizosphere soil. The changes of apatite‐P may be more governed by microbial activities (especially mycorrhizal symbiosis) which may be higher in rhizosphere soil compared to soil adhering to root mat.
Effect of kafirin protein coating on sensory quality and shelf‐life of ‘Packham's Triumph’ pears during ripening
Authors: Buchner Sonya , Kinnear Marise , Crouch Ian J , Taylor Janet , Minnaar Amanda 
Published: July 2011

AbstractBACKGROUND: Pears are exported in large quantities from South Africa, resulting in large revenues. Minimisation of quality losses once the fruit has reached the export destination is as important as following strict export and distribution protocols. Kafirin can form edible films. In this study an edible coating made from 20 g kg−1 kafirin coating solution was applied as a postharvest treatment to retard quality deterioration of ‘Packham's Triumph’ pears during storage at the typical ripening temperature (20 °C). Changes in physicochemical and sensory quality were monitored over a period of 24 days.RESULTS: The kafirin coating was unable to retard the onset of ripening but decreased the respiration rate and retarded the progression of senescence. However, moisture loss was exacerbated in the kafirin‐coated fruit during ripening at 20 °C, especially towards the end of the shelf‐life.CONCLUSION: The kafirin coating extended the eat‐ripe quality of the pears by 1–2 weeks. However, the appearance of the fruit was unacceptable after 14 days of storage in terms of wrinkled skin. Further work is needed to improve the water barrier properties of the kafirin coating by incorporating a wax or triglyceride into the coating formulation or more simply by applying a kafirin coating to waxed fruit. Copyright © 2011 Society of Chemical Industry
Asymmetric Behavior of Temperature‐Responsive Poly(N‐isopropylacrylamide) Ultrathin Layers Observed by Atomic Force Microscopy
Authors: Kumashiro Yoshikazu , Fukumori Kazuhiro , Akiyama Yoshikatsu , Itoga Kazuyoshi , Sakai Kiyotaka , Yamato Masayuki , Okano Teruo 
Published: July 2011

AbstractDuring characterization of a temperature‐responsive poly(N‐isopropylacrylamide) (PIPAAm) layer grafted onto a Si(100) substrate, atomic force microscopy (AFM) is able to probe the interactions between the microscope tip and the polymer. The modification of the AFM tip surface with octadecyltrichlorosilane (OTS) changes the interaction between the PIPAAm surface and the tip. Although a repulsive interaction is observed between a commercially available Si tip and the PIPAAm surface, a strong attractive interaction between the OTS‐modified Si tip and the surface is observed. Adhesion‐force analysis shows changes in the hydrophilic/hydrophobic character of ultrathin PIPAAm surfaces immediately after a change in temperature. The PIPAAm surface becomes hydrophobic less than 30 min after temperature increase, but requires 120 min to become hydrophilic after temperature reduction.magnified image