bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ligandless Iron‐Catalyzed Desulfinylative C–C Allylation Reactions using Grignard Reagents and Alk‐2‐enesulfonyl Chlorides (Eur. J. Org. Chem. 36/2009)
Authors: Volla Chandra M. R. , Marković Dean , Dubbaka Srinivas Reddy , Vogel Pierre 
Published: December 2009

AbstractThe cover picture shows the iron door of the vineyard of Prof. Pierre Vogel in the Lavaux region, 12 km east of Lausanne along the Geneva Lake (Lac Léman) in Western Switzerland. This region is now part of the world heritage (UNESCO). The opened door symbolizes iron‐catalyzed reactions and, in particular, a new allylation reaction of inexpensive Grignard reagents with alk‐2‐enesulfonyl chlorides obtained in a new way that is the endergonic H‐ene reaction of sulfur dioxide with simple alkenes. The lake and the mountains in the background have been here for very long and will stay as such for much longer if civilization will concentrate on more sustainable development. Vineyards have existed in this area for more than 1200 years, and the grapes produced sometimes need a little sulfur dioxide to become a good wine (a practice that is ca. 8000 years old). The chemistry disclosed here uses sulfur dioxide for allylic C–H activation. The unstable alk‐2‐enesulfinic acids so obtained are oxidized with NCS to alk‐2‐enesulfonyl chlorides. The latter are then coupled with Grignard reagents under desulfinylation conditions and iron catalysis. Sulfur dioxide can be recovered. Details are discussed in the article by P. Vogel et al. on p. 6281 ff.
Induction of lacZ mutations in Muta™Mouse primary hepatocytes
Authors: Chen Guosheng , Gingerich John , Soper Lynda , Douglas George R. , White Paul A. 
Published: December 2009

AbstractWe have developed an in vitro mutation assay using primary hepatocytes from the transgenic Muta™Mouse. Primary hepatocytes were isolated using a two‐step perfusion method with purification by Percoll, cultured, and treated with benzo[a]pyrene (BaP), 2‐amino‐1‐methyl‐6‐phenyl‐ imidazo[4,5‐b]pyridine (PhIP), 3‐nitrobenzoanthrone (3‐NBA), and cigarette smoke condensate (CSC). The mean lacZ mutant frequency (MF) for the solvent control was approximately twofold greater than the spontaneous MF observed in liver tissue. A concentration‐dependent increase in MF (up to 3.7‐fold above control) was observed following exposure to BaP. Fourfold and twofold increases in mutant frequency were observed for 3‐NBA and PhIP exposures, respectively, without the addition of any exogenous metabolic activation. A slight but statistically significant increase in lacZ MF was observed for CSC, but only at the lowest concentration. This is the first report demonstrating that mutations can be detected in cultured primary hepatocytes from Muta™Mouse. The preliminary results presented suggest that the Muta™Mouse primary hepatocyte mutagenicity assay can be used as a cost‐effective tool for screening of environmental mutagens and therapeutic products. Environ. Mol. Mutagen., 2010 © 2009 Wiley‐Liss, Inc.
Cytogenetic status in newborns and their parents in Madrid: The BioMadrid study
Authors: Lope Virginia , Pollán Marina , Fernández Mario , de León América , González Maria José , Sanz Juan Carlos , Iriso Andrés , Pérez‐Gómez Beatriz , Gil Elisa , Pérez‐Meixeira Ana María , de Paz Concha , Cisneros Margot , de Santos Amparo , Asensio Ángel , Astray Jenaro , Martínez Mercedes , García José Frutos , López‐Abente Gonzalo , García‐Sagredo José Miguel , Aragonés Nuria 
Published: December 2009

AbstractMonitoring cytogenetic damage is frequently used to assess population exposure to environmental mutagens. The cytokinesis‐block micronucleus assay is one of the most widely used methods employed in these studies. In the present study we used this assay to assess the baseline frequency of micronuclei in a healthy population of father‐pregnant woman‐newborn trios drawn from two Madrid areas. We also investigated the association between micronucleus frequency and specific socioeconomic, environmental, and demographic factors collected by questionnaire. Mercury, arsenic, lead, and cadmium blood levels were measured by atomic absorption spectrometry. The association between micronucleated cell frequency and the variables collected by questionnaire, as well as, the risk associated with the presence of elevated levels of metals in blood, was estimated using Poisson models, taking the number of micronucleated cells in 1,000 binucleated cells (MNBCs) as the dependent variable. Separate analyses were conducted for the 110 newborns, 136 pregnant women, and 134 fathers in whom micronuclei could be assessed. The mean number of micronucleated cells per 1,000 binucleated cells was 3.9, 6.5, and 6.1 respectively. Our results show a statistically significant correlation in MNBC frequency between fathers and mothers, and between parents and newborns. Elevated blood mercury levels in fathers were associated with significantly higher MNBC frequency, compared with fathers who had normal mercury levels (RR:1.21; 95%CI:1.02–1.43). This last result suggests the need to implement greater control over populations which, by reason of their occupation or life style, are among those most exposed to this metal. Environ. Mol. Mutagen., 2010. © 2009 Wiley‐Liss, Inc.
High content flow cytometric micronucleus scoring method is applicable to attachment cell lines
Authors: Bryce Steven M. , Shi Jing , Nicolette John , Diehl Marilyn , Sonders Paul , Avlasevich Svetlana , Raja Sarojini , Bemis Jeffrey C. , Dertinger Stephen D. 
Published: December 2009

AbstractA flow cytometric method for analyzing suspension cell cultures for micronucleus content has been previously reported (Avlasevich et al. [2006]: Environ Mol Mutagen 47: 56–66). The experiments described herein were undertaken to evaluate the compatibility of this method (In Vitro MicroFlow®) with attachment cells. Initially, CHO‐K1 cells were studied in nine independent experiments using mitomycin C and cyclophosphamide. The results demonstrated the effectiveness of the cell processing procedure, and also provided historical control data that were useful for setting criteria for making positive calls. Subsequently, CHO‐K1 cells were treated with methyl methanesulfonate, mitomycin C, etoposide, vinblastine sulfate, dexamethasone, and sodium chloride. Whereas the four genotoxicants were each observed to increase micronucleus frequencies, the nongenotoxicants induced no such response up to cytotoxic concentrations. Following this initial work, inter‐laboratory transferability was evaluated across three sites using a common cell staining and analysis protocol for CHO‐K1 or V79 cells that had been treated with the ten chemicals listed in Annex 3 of the OECD Draft Proposal for a New Guideline 487: In Vitro Mammalian Cell Micronucleus Test. With the exception of benzo[a]pyrene at one site, each laboratory observed increased micronucleus frequencies for the genotoxicants, whereas no significant induction occurred with the non‐genotoxicants. Interestingly, the method appeared to distinguish between genotoxic modes of action, as only aneugens increased the average micronucleus fluorescence intensity and the frequency of hypodiploid nuclei. Collectively, these data suggest that flow cytometry is capable of providing reliable micronucleus counts, and that additional information is obtained that appears to discern genotoxic modes of action. Environ. Mol. Mutagen., 2010. © 2009 Wiley‐Liss, Inc.
Bananas and petrol: further evidence on the forecasting accuracy of the ABS ‘headline’ and ‘underlying’ rates of inflation
Authors: Lenten Liam J. A. 
Published: December 2009

AbstractIn the light of the still topical nature of ‘bananas and petrol’ being blamed for driving much of the inflationary pressures in Australia in recent times, the ‘headline’ and ‘underlying’ rates of inflation are scrutinised in terms of forecasting accuracy. A general structural time‐series modelling strategy is applied to estimate models for alternative types of Consumer Price Index (CPI) measures. From this, out‐of‐sample forecasts are generated from the various models. The underlying forecasts are subsequently adjusted to facilitate comparison. The Ashley, Granger and Schmalensee (1980) test is then performed to determine whether there is a statistically significant difference between the root mean square errors of the models. The results lend weight to the recent findings of Song (2005) that forecasting models using underlying rates are not systematically inferior to those based on the headline rate. In fact, strong evidence is found that underlying measures produce superior forecasts. Copyright © 2009 John Wiley & Sons, Ltd.
A family harboring a germ‐line N‐terminal C/EBPα mutation and development of acute myeloid leukemia with an additional somatic C‐terminal C/EBPα mutation
Authors: Nanri Tomoko , Uike Naokuni , Kawakita Toshiro , Iwanaga Eisaku , Mitsuya Hiroaki , Asou Norio 
Published: December 2009

AbstractC/EBPα plays an essential role as a transcription factor in myeloid cell differentiation. Here, we describe a Japanese family in which two individuals with acute myeloid leukemia (AML) and one healthy individual had an identical 4‐base pair insertion in the N‐terminal region of CEBPA (350_351insCTAC), resulting in the termination at codon 107 (I68fsX107). The father and a son at diagnosis of AML had different in‐frame insertion mutations in the C‐terminal region of C/EBPα. These C‐terminal mutations disappeared upon remission in both patients. Interestingly, the father showed different in‐frame insertion mutations in the C‐terminal CEBPA at the time of diagnosis and relapse. These data strongly suggest that the N‐terminal C/EBPα mutation predisposes to the occurrence of a C‐terminal C/EBPα mutation as a secondary genetic hit, causing AML. © 2009 Wiley‐Liss, Inc.
Blowing ratio as an evaluation tool for velopharyngeal function after oral and oropharyngeal cancer resection
Authors: Katoh Chieri , Saitoh Miki , Tsuneyuki Miki , Tanimoto Hitoshi , Hashikawa Kazunobu , Tahara Shinya , Otsuki Naoki , Nibu Ken‐ichi 
Published: December 2009

AbstractBackground.Our objective was to evaluate the value of the blowing test to measure the speech and swallowing function after resection of oral or oropharyngeal cancer.Methods.Speech and swallowing functions of the patients after surgical resection of oral or oropharyngeal cancer were assessed by a speech intelligibility test, blowing time, questionnaires, and oropharyngeal pressures. Blowing time (pressure) ratio was determined by dividing blowing time (pressure) with open nose by blowing time (pressure) with closed nose.Results.Blowing time ratio had significant correlation with blowing pressure ratio (p = .014), score of speech intelligibility test (p = .0014), questionnaire for aspiration (p = .029), nasopharyngeal backflow (CC = 0.676, p = .032), amount of food to swallow (p = .037), and oropharyngeal pressure during swallowing (p = .024).Conclusion.The present results demonstrated the value of blowing time ratio as a simple objective tool for speech and swallowing ability related to velopharyngeal function after resection of oral and oropharyngeal cancers. © 2009 Wiley Periodicals, Inc. Head Neck, 2010
Chemotherapy alone for organ preservation in advanced laryngeal cancer
Authors: Divi Vasu , Worden Francis P. , Prince Mark E. , Eisbruch Avraham , Lee Julia S. , Bradford Carol R. , Chepeha Douglas B. , Teknos Theodoros N. , Hogikyan Norman D. , Moyer Jeffrey S. , Tsien Christina I. , Urba Susan G. , Wolf Gregory T. 
Published: December 2009

AbstractBackground.For patients with advanced laryngeal cancer, a trial was designed to determine if chemotherapy alone, in patients achieving a complete histologic complete response after a single neoadjuvant cycle, was an effective treatment with less morbidity than concurrent chemoradiotherapy.Methods.Thirty‐two patients with advanced laryngeal or hypopharyngeal cancer received 1 cycle of induction chemotherapy, and subsequent treatment was decided based on response.Results.A histologic complete response was achieved in 4 patients and were treated with chemotherapy alone. All 4 patients' cancer relapsed in the neck and required surgery and postoperative radiotherapy (RT). Twenty‐five patients were treated with concomitant chemoradiation. Three patients were treated with surgery. Overall survival and disease‐specific survival at 3 years were 68% and 78%, respectively.Conclusion.Chemotherapy alone is not feasible for long‐term control of regional disease in patients with advanced laryngeal cancer even when they achieve a histologic complete response at the primary site. © 2009 Wiley Periodicals, Inc. Head Neck, 2010
Superficial selective cervical plexus block following total thyroidectomy: A randomized trial
Authors: Kesisoglou Isaak , Papavramidis Theodossis S. , Michalopoulos Nick , Ioannidis Konstantinos , Trikoupi Anastasia , Sapalidis Konstantinos , Papavramidis Spiros T. 
Published: December 2009

AbstractBackground.Pain after thyroid surgery is of moderate intensity and short duration. Bilaterally superficial cervical plexus block (BSCPB) may reduce analgesic requirements. However, its effectiveness in decreasing pain after thyroidectomy is debated.Methods.This double‐blind, randomized placebo‐controlled study in 100 patients undergoing total thyroidectomy evaluates the effects of BSCPB done with 20 mL of 0.75% ropivacaine. Additional parecoxib was administrated immediately postoperatively and 12 hours later.Results.Postoperative pain was assessed by visual analogue rating scale. All parameters were recorded at 0, 3, 6, 9, 12, and 24 hours after surgery. The control group had higher values than the ropivacaine group at all moments (p < .05) except H12 (p = .76). Additional analgesia was needed for 7 patients (14%) in the control group and for 8 patients (16%) in the group with ropivacaine (p = .96).Conclusion.Two‐point bilateral BSCPB has a major analgesic effect on patients after total thyroidectomy, with a statistically significant reduction in postoperative pain scores. However, no significant difference was noted in the proportion of patients that required additional analgesics. © 2009 Wiley Periodicals, Inc. Head Neck, 2010
Influence of breathing resistance of heat and moisture exchangers on tracheal climate and breathing pattern in laryngectomized individuals
Authors: Scheenstra Renske J. , Muller Sara H. , Vincent Andrew , Sinaasappel Michiel , Hilgers Frans J. M. 
Published: December 2009

AbstractBackground.The aim of this study was to determine the influence of breathing resistance of heat and moisture exchangers (HMEs) on endotracheal climate and breathing pattern.Methods.Endotracheal temperature and humidity and tidal volumes were measured in 11 laryngectomized patients with a regularly used HME with “standard” breathing resistance (Provox Normal HME; R‐HME), a low breathing‐resistance HME (Provox HiFlow HME; L‐HME), and without HME.Results.Both R‐HME and L‐HME increased end‐inspiratory humidity (+5.8 and 4.7 mgH2O/L, respectively), decreased end‐inspiratory temperature (−1.6 and −1.0°C, respectively), and prolonged the exhalation breath length to approximately 0.5 seconds. The R‐HME significantly enlarged tidal volumes (0.07 L; p < .05).Conclusions.Both HMEs significantly improve tracheal climate. The R‐HME has better moistening properties and a small but significant positive effect on tidal volume. Therefore, if the higher resistance is tolerated, the R‐HME is the preferred pulmonary rehabilitation device. The L‐HME is indicated if lower breathing resistance is required. © 2009 Wiley Periodicals, Inc. Head Neck, 2010
Fatal central venous air embolism: A rare complication of esophageal dilation by rendezvous
Authors: Zald Philip B. , Andersen Peter E. 
Published: December 2009

AbstractBackground.Esophageal dilation by rendezvous is a useful technique for the treatment of complicated esophageal strictures.Methods and Results.We present a case of a 74‐year‐old man with chronic dysphagia caused by a complete cervical esophageal stricture that developed after external beam radiotherapy for treatment of papillary thyroid carcinoma. During attempted dilation using the rendezvous technique, the patient suffered a fatal pulmonary air embolism. The technique of esophageal dilation by rendezvous, complications, and risk factors for development of venous air embolism are discussed.Conclusion.To the best of our knowledge, this is the first report in the literature of fatal venous air embolism after dilation by rendezvous. © 2009 Wiley Periodicals, Inc. Head Neck, 2010
Developing an OD‐intervention metric system with the use of applied theory‐building methodology: A work/life‐intervention example
Authors: Morris Michael Lane , Storberg‐Walker Julia , McMillan Heather S. 
Published: December 2009

AbstractThis article presents a new model, generated through applied theory‐building research methods, that helps human resource development (HRD) practitioners evaluate the return on investment (ROI) of organization development (OD) interventions. This model, called organization development human‐capital accounting system (ODHCAS), identifies return‐on‐investment measures for each of the elements of the human‐capital employment life cycle that are impacted by OD interventions. We illustrate an application of the new model by using work/life (w/l) interventions as a test of the model. The contribution of this new model is fourfold:

It fills a gap in the literature by suggesting a holistic ROI framework for typically nonfinancial OD‐type interventions.
It is generated from an accepted applied theory‐building methodology.
It offers decision makers methods to develop “hard” evidence on which to evaluate w/l interventions.
It has the future potential to be expanded and used to evaluate the ROI for multiple types of OD interventions.
Numerical modeling of the groundwater flow system of the Gumera sub‐basin in Lake Tana basin, Ethiopia
Authors: Chebud Yirgalem A. , Melesse Assefa M. 
Published: December 2009

AbstractThe groundwater flow contribution into Lake Tana was simulated using Gumera River sub‐basin as a case study. Draining into Lake Tana, the Gumera sub‐basin boundary was delineated from a 90‐m Shuttle Radar Terrain Mapping (SRTM) digital elevation model (DEM) using ArcHydro tools. The sub‐basin boundary served as the divide line of the groundwater flow while stream networks were used as internal drainage lines. Input parameters such as transmissivity and hydraulic conductivity were obtained from past studies and experts' knowledge. Based on the geological information of the sub‐basin, unconfined subsurface flow condition was considered and simulated using MODular 3D finite difference ground‐water FLOW model (MODFLOW). Analytical solutions were also used for comparison purposes. The result indicates that head contours are aligned to the streams showing their relationship as a subdued form of the surface water flow, which are dictated by the morphology of the basin. This suggested the need to account groundwater contribution of the sub‐basins to Lake Tana as baseflow, and to estimate groundwater inflow from the floodplain separately. The contribution from the floodplain was estimated using a finite differences method which showed an inflow of 0·15 billion cubic meter (BCM) from rivers into the floodplain out of which 0·09 BCM is flowing out to the Lake Tana and 0·06 BCM stored in the aquifers of the floodplain. The stored quantity could meet irrigation demands as well as contribute to the preservation of the ecological structure and function of the area. The study suggests further research on validation of parameters through inverse modeling approaches and optimization of water allocations. Copyright © 2009 John Wiley & Sons, Ltd.
Towards an optical biopsy for the diagnosis of breast cancer in vivo by endogenous fluorescence spectroscopy
Authors: Alchab Lama , Dupuis Guillaume , Balleyguier Corinne , Mathieu Marie‐Christine , Fontaine‐Aupart Marie‐Pierre , Farcy René 
Published: December 2009

AbstractThe techniques of medical imaging allow the detection of suspect lesions in the breast, but they do not always evidence the malignant nature of these lesions. Breast biopsies and histological analyses are therefore implemented to establish a diagnosis. In order to reduce the number of these invasive procedures, a portable clinical system was designed based upon the excitation of Endogenous Fluorescence in vivo at 405 nm via a fiber‐optics probe included in a disposable needle of small diameter (<1 mm). From the fluorescence signal, the authors are able to discriminate between diseased and healthy areas of human breast biopsies. Stronger fluorescence intensity and systematic spectral red shift of the tumor areas were observed. These results are confirmed by confocal microscopy. This new instrument is promising for the minimally invasive diagnosis of breast tumors in vivo with an appreciable limitation of patient trauma and of operational and financial cost. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Photodynamic therapy induces microRNA‐210 and ‐296 expression in HeLa cells
Authors: Kushibiki Toshihiro 
Published: December 2009

AbstractApoptosis‐related microRNAs (miRNAs), the expression levels of which were observed to increase after photodynamic therapy (PDT), were identified as markers of PDT efficacy. miRNAs form a class of small endogenous RNAs that play important regulatory roles by targeting mRNA for cleavage or translational repression. miRNAs contribute to diverse biological processes, including development, cell growth, apoptosis, and hematopoiesis, which suggests a role in cancer development. However, no previous reports have described a relationship between apoptosis and miRNA expression after PDT. In this study, apoptosis‐related miRNA levels were characterized by real‐time PCR after PDT in HeLa cells. miR‐210 and miR‐296 expression levels increased 1 hour after PDT, although the other apoptosis‐related miRNA (miR‐7, miR‐148a, miR‐204, and miR‐216) expression levels were unchanged. Since miR‐210 is the most prominent miRNA consistently stimulated under hypoxic conditions and VEGF is capable of increasing miR‐296 expression levels, these data suggest that hypoxia induced by PDT induces miR‐210 expression, followed by an increase of VEGF expression and miR‐296 expression. These results constitute the first report of an analysis of miRNA expression after PDT using talaporfin sodium as a photosensitizer, demonstrating that miR‐210 and miR‐296 expression levels are markers of PDT efficacy in HeLa cells. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Effects of intravitreal bevacizumab (Avastin®) therapy on retrobulbar blood flow parameters in patients with neovascular age‐related macular degeneration
Authors: Mete Ahmet , Saygılı Oguzhan , Mete Alper , Bayram Metin , Bekır Necdet 
Published: December 2009

AbstractBackground.To investigate the effects of intravitreal bevacizumab on retrobulbar circulation in patients with neovascular age‐related macular degeneration (AMD).Method.Thirty patients with neovascular age‐related macular degeneration were assessed prospectively by both color Doppler imaging and fundus fluorescein angiography. Spectral Doppler analysis allowed the measurement of the maximum velocity (Vmax) and minimum velocity (Vmin) of the central retinal vein (CRV), and peak systolic (PSV), end‐diastolic (EDV) velocities of blood flows, and pulsatility index (PI) and resistance index (RI) values in the central retinal artery (CRA), nasal and temporal posterior ciliary arteries (NPCA, TPCA), and ophthalmic artery (OA). The t test for paired samples was used to compare retrobulbar blood flow values before and after intravitreal bevacizumab injection.Result.PSV and EDV of the NPCA and PSV of the TPCA were significantly decreased after intravitreal bevacizumab injection (p < 0.05). There was no statistically significant difference in the other parameters.Conclusion.Our results suggest that intravitreal bevacizumab therapy has a measurable effect on retrobulbar blood flow. © 2009 Wiley Periodicals, Inc. J Clin Ultrasound 2010