bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resolute® and xience V® polymer‐based drug‐eluting stents compared in an atherosclerotic rabbit double injury model
Authors: Van Dyck Christophe J. , Hoymans Vicky Y. , Bult Hidde , Fransen Erik , Haine Steven , Bosmans Johan M. , Timmermans Jean‐Pierre , Vrints Christiaan J. 
Published: August 2012

AimTo evaluate differences in strut coverage, inflammation and endothelialization between two second‐generation polymer‐based drug‐eluting stents (DES) in an atherosclerotic rabbit double‐injury iliac artery model at 28 days follow‐up. Methods and results: Rabbits with induced atheroma received bilateral iliac artery stents: everolimus‐eluting stent (Xience V EES; Abbott Vascular), zotarolimus‐eluting stent (Resolute ZES; Medtronic CardioVascular), or bare‐metal stent (BMS; MultiLink Vision; Abbott Vascular). After 28 days, total neointimal coverage examined by scanning electron microscopy was >98% for all three stent types. Neointimal thickness above stent struts was decreased by 50% in Xience V EES (0.06 ± 0.01 mm; P = 0.00001) compared with BMS (0.15 ± 0.03 mm) and Resolute ZES (0.12 ± 0.04 mm). Luminal area was largest for Xience V EES (3.79 ± 0.33 mm2; P = 0.0003 for Xience V EES vs. BMS), followed by Resolute ZES (3.46 ± 0.45 mm2; P = 0.083 for Resolute ZES vs. BMS) and BMS (3.07 ± 0.53 mm2). Percentage area stenosis was smallest for Xience V EES (17.23 ± 3.64%; P = 0.00001), while BMS (30.25 ± 7.48%) and Resolute ZES (30.79 ± 7.15%) did not differ. Endothelial monolayer regrowth was significantly lower in Resolute ZES (65 ± 13%) versus BMS (79 ± 11%; P = 0.004). There was no difference between Xience V EES (74 ± 10%) and BMS. Xience V EES was further associated with a lower number of inflammatory cells surrounding the stent struts (7 ± 2 per strut) in comparison to Resolute ZES (15 ± 6; P = 0.0001) and BMS (17 ± 9; P = 0.0005).ConclusionIn this atherosclerotic rabbit model, Xience V EES suppressed neointimal thickening better, with normal endothelial regrowth as compared with BMS, and less strut‐induced inflammation. © 2012 Wiley Periodicals, Inc.
Resuspension of Abiotic Particles from Ceramic Membranes
Authors: Quadt T. , Schmidt E. 
Published: August 2012

AbstractAdhesion forces are an important factor when dealing with particle resuspension. A direct measurement of these forces is not possible in industrial membrane filtration processes. However, more knowledge about the acting adhesion forces would be very helpful to use membrane filtration in an economically more efficient way. This article introduces basic experiments that should lead to an indirect, time‐resolved measurement of adhesion forces acting during the process of membrane surface regeneration. The aim is to optimize the existing regeneration process and to find new solutions for membrane regeneration.
Anterior PAR proteins function during cytokinesis and maintain DYN‐1 at the cleavage furrow in Caenorhabditis elegans
Authors: Pittman Kelly J. , Skop Ahna R. 
Published: August 2012

AbstractPAR proteins are key regulators of cellular polarity and have links to the endocytic machinery and the actin cytoskeleton. Our data suggest a unique role for PAR proteins in cytokinesis. We have found that at the onset of cytokinesis, anterior PAR‐6 and posterior PAR‐2 proteins are redistributed to the furrow membrane in a temporal and spatial manner. PAR‐6 and PAR‐2 localize to the furrow membrane during ingression but PAR‐2‐GFP is distinct in that it is excluded from the extreme tip of the furrow. Once the midbody has formed, PAR‐2‐GFP becomes restricted to the midbody region (the midbody plus the membrane flanking it). Depletion of both anterior PAR proteins, PAR‐3 and PAR‐6, led to an increase in multinucleate embryos, suggesting that the anterior PAR proteins are necessary during cytokinesis and that PAR‐3 and PAR‐6 function in cytokinesis may be partially redundant. Lastly, anterior PAR proteins play a role in the maintenance of DYN‐1 in the cleavage furrow. Our data indicate that the PAR proteins are involved in the events that occur during cytokinesis and may play a role in promoting the membrane trafficking and remodeling events that occur during this time. © 2012 Wiley Periodicals, Inc
Centralspindlin and chromosomal passenger complex behavior during normal and Rappaport furrow specification in echinoderm embryos
Authors: Argiros Haroula , Henson Lauren , Holguin Christiana , Foe Victoria , Shuster Charles Bradley 
Published: August 2012

AbstractThe chromosomal passenger (CPC) and Centralspindlin complexes are essential for organizing the anaphase central spindle and providing cues that position the cytokinetic furrow between daughter nuclei. However, echinoderm zygotes are also capable of forming “Rappaport furrows” between asters positioned back‐to‐back without intervening chromosomes. To understand how these complexes contribute to normal and Rappaport furrow formation, we studied the localization patterns of Survivin and mitotic‐kinesin‐like‐protein1 (MKLP1), members respectively of the CPC and the Centralspindlin complex, and the effect of CPC inhibition on cleavage in mono‐ and binucleate echinoderm zygotes. In zygotes, Survivin initially localized to metaphase chromosomes, upon anaphase onset relocalized to the central spindle and then, together with MKLP1 spread towards the equatorial cortex in an Aurora‐dependent manner. Inhibition of Aurora kinase activity resulted in disruption of central spindle organization and furrow regression, although astral microtubule elongation and furrow initiation were normal. In binucleate cells containing two parallel spindles MKLP1 and Survivin localized to the plane of the former metaphase plate, but were not observed in the secondary cleavage plane formed between unrelated spindle poles, except when chromosomes were abnormally present there. However, the secondary furrow was sensitive to Aurora inhibition, indicating that Aurora kinase may still contribute to furrow ingression without chromosomes nearby. Our results provide insights that reconcile classic micromanipulation studies with current molecular understanding of furrow specification in animal cells. © 2012 Wiley Periodicals, Inc
Symmetric cell division of the mouse zygote requires an actin network
Authors: Chew Ting Gang , Lorthongpanich Chanchao , Ang Wei Xia , Knowles Barbara B. , Solter Davor 
Published: August 2012

AbstractPositioning of the cleavage plane is regulated to ensure proper animal development. Most animal cells rely on the astral microtubules to position the mitotic spindle, which in turn specifies the cleavage plane. The mouse zygote lacks discernible astral microtubules but still divides symmetrically. Here, we demonstrate a cloud‐like accumulation of F‐actin surrounds the spindle in zygotes and when this actin network is disassembled, the spindle assumes an off‐center position, and the resulting zygote divides asymmetrically into two unequal size blastomeres. Interestingly, when the spindle is micromanipulated to the subcortical region, the zygote without the actin network is unable to reposition the spindle and cleavage plane at the cell center. This study reveals that an actin network maintains the central spindle position in anastral mitosis, and ensures the first embryonic mitosis is symmetrical. © 2012 Wiley Periodicals, Inc
Randomly biased walks on subcritical trees
Authors: Ben Arous Gérard , Hammond Alan 
Published: August 2012

AbstractAs a model of trapping by biased motion in random structure, we study the time taken for a biased random walk to return to the root of a subcritical Galton‐Watson tree. We do so for trees in which these biases are randomly chosen, independently for distinct edges, according to a law that satisfies a logarithmic nonlattice condition. The mean return time of the walk is in essence given by the total conductance of the tree. We determine the asymptotic decay of this total conductance, finding it to have a pure power‐law decay. In the case of the conductance associated to a single vertex at maximal depth in the tree, this asymptotic decay may be analyzed by the classical defective renewal theorem, due to the nonlattice edge‐bias assumption. However, the derivation of the decay for total conductance requires computing an additional constant multiple outside the power law that allows for the contribution of all vertices close to the base of the tree. This computation entails a detailed study of a convenient decomposition of the tree under conditioning on the tree having high total conductance. As such, our principal conclusion may be viewed as a development of renewal theory in the context of random environments.For randomly biased random walks on a supercritical Galton‐Watson tree with positive extinction probability, our main results may be regarded as a description of the slowdown mechanism caused by the presence of subcritical trees adjacent to the backbone that may act as traps that detain the walker. Indeed, this conclusion is exploited in a sequel by the second author to obtain a stable limiting law for walker displacement in such a tree. © 2012 Wiley Periodicals, Inc.
Birth timing and the mother–infant relationship predict variation in infant behavior and physiology
Authors: Vandeleest Jessica J. , Mendoza Sally P. , Capitanio John P. 
Published: August 2012

AbstractThe current study explored whether birth timing, known to influence the mother–infant relationship, also affected infant physiology up to 9 months later and infant behavior at weaning. Infant blood samples were collected at 5.75 and 8.75 months of age to assess functioning of the hypothalamic–pituitary–adrenal axis as well as the antibody response to a Cholera vaccination. Path analysis indicated infants born late in the birth season had less Relaxed relationships with their mothers. A less‐Relaxed relationship was associated with greater infant Positive Engagement and Distress, which were negatively correlated, suggesting infants may have different strategies of coping with this type of relationship. Low Relaxed scores were also associated with higher infant cortisol concentrations at 5.75 months, which was associated with a reduced immune response to a vaccination 3 months later. Together these results indicate that the influence of birth timing on the mother–infant relationship may have consequences for infant development. © 2012 Wiley Periodicals, Inc. Dev Psychobiol 55: 829–837, 2013.
Craniofacial abnormalities result from knock down of nonsyndromic clefting gene, crispld2, in zebrafish
Authors: Yuan Qiuping , Chiquet Brett T. , DeVault Laura , Warman Matthew L. , Nakamura Yukio , Swindell Eric C. , Hecht Jacqueline T. 
Published: August 2012

AbstractNonsyndromic cleft lip and palate (NSCLP), a common birth defect, affects 4,000 newborns in the US each year. Previously, we described an association between CRISPLD2 and NSCLP and showed Crispld2 expression in the murine palate. These results suggested that a perturbation in CRISPLD2 activity affects craniofacial development. Here, we describe crispld2 expression and the phenotypic consequence of its loss of function in zebrafish. crispld2 was expressed at all stages of zebrafish morphogenesis examined and localized to the rostral end by 1‐day postfertilization. Morpholino knockdown of crispld2 resulted in significant jaw and palatal abnormalities in a dose‐dependent manner. Loss of crispld2 caused aberrant patterning of neural crest cells (NCC) suggesting that crispld2 is necessary for normal NCC formation. Altogether, we show that crispld2 plays a significant role in the development of the zebrafish craniofacies and alteration of normal protein levels disturbs palate and jaw formation. These data provide support for a role of CRISPLD2 in NSCLP. genesis 50:871–881, 2012. © 2012 Wiley Periodicals, Inc.
Trideuteriomethoxylation of Aryl and Heteroaryl Halides
Authors: Dash Pragyanditi , Janni Manojkumar , Peruncheralathan S. 
Published: August 2012

AbstractDirect access to trideuteriomethoxylated aromatic and heteroaromatic compounds has been developed. Various aryl and heteroaryl halides underwent d3‐methoxylation under mild reaction conditions by using a catalyst system composed of the commercially available monodentate phosphane ligand tBuXPhos and Pd(OAc)2. Inexpensive CD3OD served as an efficient trideuteriomethoxylating agent.
Man‐Tailored Biomimetic Sensor of Molecularly Imprinted Materials for the Potentiometric Measurement of Rivastigmine in Tablets and Biological Fluids and Employing the Taguchi Optimization Methodology to Optimize the MIP‐Based Membranes
Authors: Arvand Majid , Fallahi Parisa 
Published: August 2012

AbstractThis work proposes a novel biomimetic sensor for the potentiometric transduction of rivastigmine based on molecularly imprinted polymer (MIP). Using the Taguchi method, this study analyzed the optimum conditions for preparing the MIP‐based membranes. The rank order of each controllable factor was also determined. MIP‐based membranes exhibited a Nernstian response (30.7±1.1 mV decade−1) in a concentration range from 1.0×10−5 to 1.0×10−2 mol L−1 with a LOD of 6.3×10−6 mol L−1. The sensor was successfully applied to the determination of rivastigmine concentrations in human serum, plasma, urine, rat brain and tablets.
Disposable Electrochemical Magnetoimmunosensor for the Determination of Troponin T Cardiac Marker
Authors: de Ávila Berta Esteban‐Fernández , Escamilla‐Gómez Vanessa , Campuzano Susana , Pedrero María , Pingarrón José M. 
Published: August 2012

AbstractThe preparation and performance of a disposable amperometric magnetoimmunosensor for the specific detection and quantification of human cardiac Troponin T is reported. The immunosensor design involves binding of streptavidin‐ magnetic beads to a specific biotinylated capture antibody and detection with a HRP‐labeled antibody. The resulting modified‐MBs are magnetically captured on the surface of a disposable gold screen‐printed electrode and the amperometric response obtained at −0.10 V upon addition of 3,3′,5,5′‐tetramethylbenzidine and H2O2 is used to monitor the affinity reaction. This methodology achieves a detection limit of 0.017 ng mL−1, a good selectivity and has been successfully tested with serum samples.
A HEALTH PRODUCTION MODEL WITH ENDOGENOUS RETIREMENT
Authors: Galama Titus , Kapteyn Arie , Fonseca Raquel , Michaud Pierre‐Carl 
Published: August 2012

ABSTRACTWe formulate a stylized structural model of health, wealth accumulation and retirement decisions building on the human capital framework of health and derive analytic solutions for the time paths of consumption, health, health investment, savings and retirement. We argue that the literature has been unnecessarily restrictive in assuming that health is always at the ‘optimal’ health level. Exploring the properties of corner solutions, we find that advances in population health decrease the retirement age, whereas at the same time, individuals retire when their health has deteriorated. This potentially explains why retirees point to deteriorating health as an important reason for early retirement, whereas retirement ages have continued to fall in the developed world, despite continued improvements in population health and mortality. In our model, workers with higher human capital invest more in health and, because they stay healthier, retire later than those with lower human capital whose health deteriorates faster. Copyright © 2012 John Wiley & Sons, Ltd.
Effects of a red tide on the structure of estuarine fish assemblages in northeastern Brazil
Authors: Amorim Reis‐Filho José , da Silva Eduardo M. , de Anchieta Cintra da Costa Nunes José , Barros Francisco 
Published: August 2012

AbstractThe present study evaluated changes in an estuarine fish community caused by blooms of the dinoflagellate Akashiwo sanguinea. Samples were collected before, during and after the red tide using seine nets and surface and bottom nets in three areas near the affected area. The area was around the mouth of the Paraguaçu River. A total of 144 samples were collected, containing 1,989 individuals, with a total weight of 51.4 kg, belonging to 42 species in 29 families. During the red tide, fish density, richness and biomass decreased significantly. Atherinella brasiliensis and Sphoeroides greeleyi were the most abundant species during the red tide, indicating some possible resistance to the effects of the red tide. One month after the red tide, there was a rapid recovery of the density, biomass and richness of fish, and Cetengraulis edentulus was the most captured species. An important result of the present study is the finding that the dynamics of small populations of fish are often influenced by fortuitous events, and stochasticity may dominate. The establishment of a continuous and adequate monitoring program in the area may contribute to understanding the effects of red tides on fish population dynamics. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Seasonal dynamics and composition of cladoceran and copepod assemblages in ponds of a Hungarian cutaway peatland
Authors: Vad Csaba Ferenc , Horváth Zsófia , Kiss Keve Tihamér , Ács Éva , Török Júlia Katalin , Forró László 
Published: August 2012

AbstractSeasonal dynamics of cladoceran and copepod zooplankton were studied over a one‐year period in five permanent ponds of a cutaway peatland, situated in the Danube−Tisza Interfluve, Central Hungary. 17 cladoceran, 11 cyclopoid copepod and 6 harpacticoid copepod species were identified and most of them were typical of small lowland ponds. Nevertheless, some taxa like Cyclops insignis, Ceriodaphnia setosa and Macrocyclops distinctus are considered to be rare in Hungary. The microcrustacean assemblages exhibited apparent seasonal succession with typical seasonal species. There appears to be at least two main successional patterns in the five ponds. After general cyclopoid copepod dominance in winter (Cyclops strenuus and Cyclops insignis), at sites with higher proportion of open water and submerged vegetation, spring was characterized by the dominance of the large cladoceran Daphnia curvirostris, which declined during summer, when microcrustacean assemblages composed mainly of smaller, littoral cladocerans. At these sites, species richness and diversity reached their maximum in autumn. In the case of duckweed covered ponds, succession led to less diverse autumn assemblages with fewer species, dominated by Simocephalus exspinosus. Our results draw the attention to the importance of long‐term investigations and the often neglected winter sampling in the accurate evaluation of species richness (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Hypoxia‐induced downregulation of XIAP in trophoblasts mediates apoptosis via interaction with IMUP‐2: Implications for placental development during pre‐eclampsia
Authors: Jeon Su Yeon , Lee Hyun‐Jung , Na Kyu‐Hwan , Cha Dong‐Hyun , Kim Jin Kyeoung , Park Jong‐Wan , Yoon Tae Ki , Kim Gi Jin 
Published: August 2012

AbstractThe regulation of trophoblast apoptosis is essential for normal placentation, and increased placental trophoblast cell apoptosis is the cause of pathologies such as intrauterine growth retardation (IUGR) and pre‐eclampsia. X‐linked inhibitor of apoptosis (XIAP) is expressed in trophoblasts, but little is known about the role of XIAP in placental development. In the present study, the function of XIAP in the placenta and in HTR‐8/SVneo trophoblasts under hypoxic conditions was examined. In addition, the correlation between XIAP and immortalization‐upregulated protein‐2 (IMUP‐2) was demonstrated in HTR‐8/SVneo trophoblasts under hypoxia, based on a previous study showing that increased IMUP‐2 induces trophoblast apoptosis and pre‐eclampsia. XIAP was downregulated in pre‐eclamptic placentas (P < 0.05). In HTR‐8/SVneo trophoblasts, XIAP expression was decreased and the expression of apoptosis‐related genes was increased in response to hypoxia. Ectopic expression of hypoxia inducible factor (HIF)‐1α in HRT‐8 SV/neo cells induced the nuclear translocation of XIAP and alterations of XIAP protein stability. Furthermore, hypoxia induced nuclear translocated XIAP co‐localized with upregulated IMUP‐2 in trophoblast nuclei, and the interaction between XIAP and IMUP‐2 induced apoptosis in HRT‐8 SV/neo cells. The present results suggest that hypoxia‐induced down‐regulation of XIAP mediates apoptosis in trophoblasts through interaction with increased IMUP‐2, and that this mechanism underlies the pathogenesis of pre‐eclampsia. J. Cell. Biochem. 114: 89–98, 2012. © 2012 Wiley Periodicals, Inc.
Multiphoton tomography visualizes collagen fibers in the tumor microenvironment that maintain cancer‐cell anchorage and shape
Authors: Uchugonova Aisada , Zhao Ming , Weinigel Martin , Zhang Yong , Bouvet Michael , Hoffman Robert M. , König Karsten 
Published: August 2012

AbstractSecond harmonic generation (SHG) multiphoton imaging can visualize fibrillar collagen in tissues. SHG has previously shown that fibrillar collagen is altered in various types of cancer. In the present study, in vivo high resolution SHG multi‐photon tomography in living mice was used to study the relationship between cancer cells and intratumor collagen fibrils. Using green fluorescent protein (GFP) to visualize cancer cells and SHG to image collagen, we demonstrated that collagen fibrils provide a scaffold for cancer cells to align themselves and acquire optimal shape. These results suggest a new paradigm for a stromal element of tumors: their role in maintaining anchorage and shape of cancer cells that may enable them to proliferate. J. Cell. Biochem. 114: 99–102, 2012. © 2012 Wiley Periodicals, Inc.
D2 dopamine receptor antagonist raclopride induces non‐canonical autophagy in cardiac myocytes
Authors: Yan Hao , Li Wen‐lin , Xu Jian‐jun , Zhu Shu‐qiang , Long Xiang , Che Jian‐peng 
Published: August 2012

AbstractCell death by autophagy is an important means of maintaining cellular homeostasis in adult cardiac myocytes. Autophagy was previously shown to exert a cardioprotective effect, suggesting that modulation of autophagy pathways is a potential therapeutic strategy in the treatment of heart disease. Although dopamine is known to induce autophagy in neuroblastoma cells, the underlying mechanism and the types of dopamine receptors involved in this process remain unclear. In this study, we used various dopamine receptor antagonists and agonists to identify the specific dopamine receptor that mediates induction of autophagy. We evaluated autophagy induction by the expression of autophagy markers in neonatal rat ventricular cardiac myocytes. We evaluated intracellular calcium levels using Fluo‐3/AM and demonstrated autophagy‐induced morphological changes in cardiac myocytes using electron microscopy. We also examined the pathway for dopamine‐induced autophagy using RNAi‐mediated gene knockdown. Raclopride, the well‐documented D2 receptor antagonist, significantly upregulated autophagy in cardiac myocytes via an mTOR‐independent pathway. There was no difference in intracellular calcium levels between raclopride‐treated cells and untreated cells. siRNA‐mediated knockdown of Rab9 resulted in decreased expression of autophagy markers in raclopride‐treated cells. Interestingly, siRNA‐mediated knockdown of Atg7 resulted in a significant increase in Rab9 levels in raclopride‐treated cells, suggesting that blocking the classical autophagy pathway results in activation of an alternative pathway. Our study suggests that (1) the D2 dopamine receptor plays a role in autophagy and (2) raclopride mediated a non‐canonical autophagy pathway in cardiac myocytes via Rab9. J. Cell. Biochem. 114: 103–110, 2012. © 2012 Wiley Periodicals, Inc.