bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of RNA binding motif proteins essential for cardiovascular development
Authors: Maragh Samantha , Miller Ronald A , Bessling Seneca L , McGaughey David M , Wessels Marja W , de Graaf Bianca , Stone Eric A , Bertoli-Avella Aida M , Gearhart John D , Fisher Shannon , McCallion Andrew S 
Published: October 2011

Abstract

Background
We recently identified Rbm24 as a novel gene expressed during mouse cardiac development. Due to its tightly restricted and persistent expression from formation of the cardiac crescent onwards and later in forming vasculature we posited it to be a key player in cardiogenesis with additional roles in vasculogenesis and angiogenesis.

Results
To determine the role of this gene in cardiac development, we have identified its zebrafish orthologs (rbm24a and rbm24b), and functionally evaluated them during zebrafish embryogenesis. Consistent with our underlying hypothesis, reduction in expression of either ortholog through injection of morpholino antisense oligonucleotides results in cardiogenic defects including cardiac looping and reduced circulation, leading to increasing pericardial edema over time. Additionally, morphant embryos for either ortholog display incompletely overlapping defects in the forming vasculature of the dorsal aorta (DA), posterior caudal vein (PCV) and caudal vein (CV) which are the first blood vessels to form in the embryo. Vasculogenesis and early angiogenesis in the trunk were similarly compromised in rbm24 morphant embryos at 48 hours post fertilization (hpf). Subsequent vascular maintenance was impaired in both rbm24 morphants with substantial vessel degradation noted at 72 hpf.

Conclusion
Taken collectively, our functional data support the hypothesis that rbm24a and rbm24b are key developmental cardiac genes with unequal roles in cardiovascular formation.
A comparative assessment of mandible shape in a consomic strain panel of the house mouse (Mus musculus) - implications for epistasis and evolvability of quantitative traits
Authors: Boell Louis , Gregorova Sona , Forejt Jiri , Tautz Diethard 
Published: October 2011

Abstract

Background
Expectations of repeatedly finding associations between given genes and phenotypes have been borne out by studies of parallel evolution, especially for traits involving absence or presence of characters. However, it has rarely been asked whether the genetic basis of quantitative trait variation is conserved at the intra- or even at the interspecific level. This question is especially relevant for shape, where the high dimensionality of variation seems to require a highly complex genetic architecture involving many genes.

Results
We analyse here the genetic effects of chromosome substitution strains carrying M. m. musculus chromosomes in a largely M. m. domesticus background on mandible shape and compare them to the results of previously published QTL mapping data between M. m. domesticus strains. We find that the distribution of genetic effects and effect sizes across the genome is consistent between the studies, while the specific shape changes associated with the chromosomes are different. We find also that the sum of the effects from the different M. m. musculus chromosomes is very different from the shape of the strain from which they were derived, as well as all known wild type shapes.

Conclusions
Our results suggest that the relative chromosome-wide effect sizes are comparable between the long separated subspecies M. m. domesticus and M. m. musculus, hinting at a relative stability of genes involved in this complex trait. However, the absolute effect sizes and the effect directions may be allele-dependent, or are context dependent, i.e. epistatic interactions appear to play an important role in controlling shape.
Cancer associated epigenetic transitions identified by genome-wide histone methylation binding profiles in human colorectal cancer samples and paired normal mucosa
Authors: Enroth Stefan , Rada-Iglesisas Alvaro , Andersson Robin , Wallerman Ola , Wanders Alkwin , Påhlman Lars , Komorowski Jan , Wadelius Claes 
Published: October 2011

AbstractBackgroundDespite their well-established functional roles, histone modifications have received less attention than DNA methylation in the cancer field. In order to evaluate their importance in colorectal cancer (CRC), we generated the first genome-wide histone modification profiles in paired normal colon mucosa and tumor samples.MethodsChromatin immunoprecipitation and microarray hybridization (ChIP-chip) was used to identify promoters enriched for histone H3 trimethylated on lysine 4 (H3K4me3) and lysine 27 (H3K27me3) in paired normal colon mucosa and tumor samples from two CRC patients and for the CRC cell line HT29.ResultsBy comparing histone modification patterns in normal mucosa and tumors, we found that alterations predicted to have major functional consequences were quite rare. Furthermore, when normal or tumor tissue samples were compared to HT29, high similarities were observed for H3K4me3. However, the differences found for H3K27me3, which is important in determining cellular identity, indicates that cell lines do not represent optimal tissue models. Finally, using public expression data, we uncovered previously unknown changes in CRC expression patterns. Genes positive for H3K4me3 in normal and/or tumor samples, which are typically already active in normal mucosa, became hyperactivated in tumors, while genes with H3K27me3 in normal and/or tumor samples and which are expressed at low levels in normal mucosa, became hypersilenced in tumors.ConclusionsGenome wide histone modification profiles can be used to find epigenetic aberrations in genes associated with cancer. This strategy gives further insights into the epigenetic contribution to the oncogenic process and may identify new biomarkers.
Doctors and local media: a synergy for public health information? A controlled trial to evaluate the effects of a multifaceted campaign on antibiotic prescribing (protocol)
Authors:   LOcal Campaign on Antibiotics ALliance (LOCAAL) study group
Published: October 2011

Abstract

Background
Use of information campaigns and educational interventions directed to citizens and supported by physicians, aimed at promoting the appropriate use of medicines, have been evaluated by several studies with conflicting results. These interventions are potentially relevant, favouring the reduction of unnecessary use of medicines and related risks. Several studies have specifically evaluated the promotion of the appropriate use of antibiotics in adults and children, with variable results. A controlled study is proposed to evaluate the feasibility and effectiveness of a multifaceted intervention aimed at reducing antibiotic prescription by increasing awareness on risks of their unnecessary use.

Methods/design
Information will be provided to citizens through several media (posters, local TV, radio and newspapers, video terminals, websites of Local Health Authorities). Brochures with information on expected benefits and risks of antibiotics will be also available, either with direct access in waiting rooms and pharmacies or handed out and mediated by doctors. Physicians and pharmacists will get specific data on local antibiotic resistance. A small group of representative doctors have also actively participated in defining the campaign key messages. A sample of general practitioners and paediatricians will be trained in patient counselling strategies.
The information campaign will be implemented in two Provinces of Emilia-Romagna during the fall-winter season (November 2011-February 2012). Change in the overall prescribing rate of antibiotics (expressed as DDD per 1000 inhabitants/day) in the intervention area will be compared versus other areas in the same Region. Knowledge and attitudes of the general population will be evaluated through a phone and internet survey on a representative sample.

Discussion
While the campaign messages will be mainly directed to the general population, doctors' prescribing will be assessed. The main rationale for this apparent discrepancy lies in the influence patients may have on physicians' prescribing behaviour (directly or indirectly) and in physicians' endorsement of the campaign goals, considering their participation in its design.
This study could observe a reduction lower than 5% in the prescribing rate of antibiotics. Such a reduction would be of public health relevance and would determine average savings of almost twice as much as the campaign costs.
Protocol for Northern Ireland Caries Prevention in Practice Trial (NIC-PIP) trial: a randomised controlled trial to measure the effects and costs of a dental caries prevention regime for young children attending primary care dental services
Authors: Tickle Martin , Milsom Keith M , Donaldson Michael , Killough Seamus , O'Neill Ciaran , Crealey Grainne , Sutton Matthew , Noble Solveig , Greer Margaret , Worthington Helen V 
Published: October 2011

Abstract

Background
Dental caries is a persistent public health problem with little change in the prevalence in young children over the last 20 years. Once a child contracts the disease it has a significant impact on their quality of life. There is good evidence from Cochrane reviews including trials that fluoride varnish and regular use of fluoride toothpaste can prevent caries.
The Northern Ireland Caries Prevention in Practice Trial (NIC-PIP) trial will compare the costs and effects of a caries preventive package (fluoride varnish, toothpaste, toothbrush and standardised dental health education) with dental health education alone in young children.

Methods/Design
A randomised controlled trial on children initially aged 2 and 3 years old who are regular attenders at the primary dental care services in Northern Ireland. Children will be recruited and randomised in dental practices. Children will be randomised to the prevention package of both fluoride varnish (twice per year for three years), fluoride toothpaste (1,450 ppm F) (supplied twice per year), a toothbrush (supplied twice a year) or not; both test and control groups receive standardised dental health education delivered by the dentist twice per year. Randomisation will be conducted by the Belfast Trust Clinical Research Support Centre ([CRSC] a Clinical Trials Unit).
1200 participants will be recruited from approximately 40 dental practices. Children will be examined for caries by independent dental examiners at baseline and will be excluded if they have caries. The independent dental examiners will examine the children again at 3 years blinded to study group.
The primary end-point is whether the child develops caries (cavitation into dentine) or not over the three years. One secondary outcome is the number of carious surfaces in the primary dentition in children who experience caries. Other secondary outcomes are episodes of pain, extraction of primary teeth, other adverse events and costs which will be obtained from parental questionnaires.

Discussion
This is a pragmatic trial conducted in general dental practice. It tests a composite caries prevention intervention, which represents an evidence based approach advocated by current guidance from the English Department of Health which is feasible to deliver to all low risk (caries free) children in general dental practice. The trial will provide valuable information to policy makers and clinicians on the costs and effects of caries prevention delivered to young children in general dental practice.

Trial registration
EudraCT No: 2009 - 010725 - 39
ISRCTN: ISRCTN36180119

Ethics Reference No: 09/H1008/93:
Association of ABO blood group with severe falciparum malaria in adults: case control study and meta-analysis
Authors: Panda Aditya K , Panda Santosh K , Sahu Aditya N , Tripathy Rina , Ravindran Balachandran , Das Bidyut K 
Published: October 2011

Abstract

Background
Erythrocyte-associated antigenic polymorphisms or their absence have perhaps evolved in the human population to protect against malarial infection. Studies in various populations consistently demonstrate that blood group 'O' confers resistance against severe falciparum infection. In India, Odisha state has one of the highest incidences of Plasmodium falciparum infection and contributes to the highest number of deaths by falciparum malaria. This study aims to evaluate the relationship between ABO blood group and severe malaria in an adult population at the tertiary care centre in Odisha.

Methods
A total of 353 P. falciparum infected subjects and 174 healthy controls were screened for ABO blood group. Falciparum-infected individuals were categorized as severe malaria and uncomplicated malaria. Severe malaria was further clinically phenotyped into cerebral malaria, non-cerebral severe malaria and multi-organ dysfunction. A meta-analysis was performed to assess the role of ABO blood group in severe malaria.

Results
Frequency of blood group 'B' was significantly higher in patients with severe malaria compared to the uncomplicated cases (P < 0.0001; OR = 4.09) and healthy controls (P < 0.0001; OR = 2.79). Irrespective of the level of clinical severity, blood group 'B' was significantly associated with cerebral malaria (P < 0.0001; OR = 5.95), multi-organ dysfunction (P < 0.0001; OR = 4.81) and non-cerebral severe malaria patients (P = 0.001; OR = 3.02) compared to the uncomplicated category. Prevalence of 'O' group in uncomplicated malaria (P < 0.0001; OR = 2.81) and healthy controls (P = 0.0003; OR = 2.16) was significantly high compared to severe malaria. Meta-analysis of previous studies, including the current one, highlighted the protective nature of blood group 'O' to severe malaria (P = 0.01). On the other hand, carriers of blood group 'A' (P = 0.04) and 'AB' (P = 0.04) were susceptible to malaria severity.

Conclusions
Results of the current study indicate that blood group 'O' is associated with reduced and 'B' blood group with increased risk of development of severe malaria in Odisha, India. Meta-analysis also supports the protective nature of blood group 'O' from severe falciparum infection.
Postprandial differences in the plasma metabolome of healthy Finnish subjects after intake of a sourdough fermented endosperm rye bread versus white wheat bread
Authors: Bondia-Pons Isabel , Nordlund Emilia , Mattila Ismo , Katina Kati , Aura Anna-Marja , Kolehmainen Marjukka , Orešič Matej , Mykkänen Hannu , Poutanen Kaisa 
Published: October 2011

Abstract

Background
The mechanism behind the lowered postprandial insulin demand observed after rye bread intake compared to wheat bread is unknown. The aim of this study was to use the metabolomics approach to identify potential metabolites related to amino acid metabolism involved in this mechanism.

Methods
A sourdough fermented endosperm rye bread (RB) and a standard white wheat bread (WB) as a reference were served in random order to 16 healthy subjects. Test bread portions contained 50 g available carbohydrate. In vitro hydrolysis of starch and protein were performed for both test breads. Blood samples for measuring glucose and insulin concentrations were drawn over 4 h and gastric emptying rate (GER) was measured. Changes in the plasma metabolome were investigated by applying a comprehensive two-dimensional gas chromatography coupled to time-of-flight mass spectrometry metabolomics platform (GC×GC-TOF-MS).

Results
Plasma insulin response to RB was lower than to WB at 30 min (P = 0.004), 45 min (P = 0.002) and 60 min (P < 0.001) after bread intake, and plasma glucose response was significantly higher at time point 90 min after RB than WB intake (P = 0.045). The starch hydrolysis rate was higher for RB than WB, contrary to the in vitro protein digestibility. There were no differences in GER between breads. From 255 metabolites identified by the metabolomics platform, 26 showed significant postprandial relative changes after 30 minutes of bread intake (p and q values < 0.05). Among them, there were changes in essential amino acids (phenylalanine, methionine, tyrosine and glutamic acid), metabolites involved in the tricarboxylic acid cycle (alpha-ketoglutaric, pyruvic acid and citric acid) and several organic acids. Interestingly, the levels of two compounds involved in the tryptophan metabolism (picolinic acid, ribitol) significantly changed depending on the different bread intake.

Conclusions
A single meal of a low fibre sourdough rye bread producing low postprandial insulin response brings in several changes in plasma amino acids and their metabolites and some of these might have properties beneficial for health.
Gender Differences in Children's and Parents' Social Support Perceptions
Authors: Matthewson Mandy , Smith Rosanne Burton , Montgomery Iain 
Published: October 2011

AbstractThere are limited studies investigating gender differences within the father–child and mother–child relationships with regard to social support provisions within these relationships. This study aimed to explore gender unity in children's and parents' perceptions of social support within the parent–child relationship. The participants included 91 families who completed measures of social support satisfaction and social support effectiveness. Results were contrary to the gender unity assumption. Findings indicated that mothers and fathers provide different types of support to their children regardless of their child's sex. Further, this study demonstrated the importance of father–child interactions and the need to further investigate the types of support fathers provide their children. This study explored gender differences in social support perceptions within the parent–child relationship. It investigated if mothers and fathers perceive the support they provide their children with is differentiated according to whether their child is a son or a daughter (i.e., gender unity). In this study, gender unity relates to whether parents consider themselves to be more effective in their support provisions to their same-sex child than to their opposite-sex child. Moreover, gender unity also pertains to whether children are more satisfied with the support provided by their same-sex parent than their opposite-sex parent. It is therefore important to define social support in order to understand it within the context of the parent-child relationship.
The Indirect Relationship Between Rumination, Shame And Depression: A Mediation Analysis for Those Experiencing Relationship Difficulties
Authors: Rice Simon , Fallon Barry 
Published: October 2011

AbstractDifficulties in intimate partner relationships are known to have detrimental effects on mental health. The association between relationship difficulties and depression is particularly strong for individuals with a tendency for rumination. While the link between rumination and depression has long been established, the indirect effects of shame and emotion regulation on this relationship remain less clear. The present study reports on longitudinal data of 65 participants (36 males) who had experienced recent relationship difficulties with their partner. Respondents provided Time 1 data for rumination (brooding and reflection), shame (personal feelings of shame) and relationship difficulties. Approximately 15 weeks later, Time 2 data was collected for emotion regulation (cognitive reappraisal, expressive suppression), relationship difficulties and depression. Mediation analyses with bias-corrected bootstrapping indicated that shame fully mediated the relationship between brooding and depression, and partially mediated the relationship between reflection and depression. The indirect effects remained significant with covariates (relationship difficulties at Time 1, and cognitive reappraisal and expressive suppression at Time 2). Results suggest that self-evaluations related to shame contribute to the relationship between rumination and depression, independent of affect regulation. Implications for the role of shame in relationship difficulties are discussed.
Adult Attachment Style Across Individuals and Role-Relationships: Avoidance is Relationship-Specific, But Anxiety Shows Greater Generalisability
Authors: Moreira João M. 
Published: October 2011

AbstractA generalisability study examined the hypotheses that avoidant attachment, reflecting the representation of others, should be more relationship-specific (vary across relationships more than across individuals), while attachment anxiety, reflecting self-representation, should be more generalisable across a person's relationships. College students responded to 6-item questionnaire measures of these variables for 5 relationships (mother, father, best same-gender friend, romantic partner or best opposite-gender friend, other close person), on 3 (N= 120) or 2 (N= 77) occasions separated by a few weeks. Results supported the hypotheses, with the person variance component being larger than the relationship-specific component for anxiety, and the opposite happening for avoidance. Anxiety therefore seems not to be as relationship-specific as previous research suggested. Possible reasons for discrepancies between the current and previous studies are discussed.
Hydroxysteroid sulfotransferase 2B1b expression and localization in normal human brain
Authors: Salman Emily D. , Faye-Petersen Ona , Falany Charles N. 
Published: October 2011

Abstract
Steroid sulfonation in the human brain has not been well characterized. The major sulfotransferase (SULT) isoforms that conjugate steroids in humans are SULT1E1, SULT2A1, and SULT2B1b. SULT2B1b catalyzes the sulfonation of 3β-hydroxysteroids, including neurosteroids dehydroepiandrosterone and pregnenolone, as well as cholesterol and several hydroxycholesterols. SULT2B1b mRNA and protein expression were detected in adult and fetal human brain sections, whereas neither mRNA, nor protein expression were identified for SULT1E1 or SULT2A1. Using immunohistochemical analysis, SULT2B1b expression was detected in neurons and oligodendrocytes in adult brain and in epithelial tissues in 28-week-old fetal brain. Sulfonation of cholesterol, oxysterols, and neurosteroids in the brain is apparently catalyzed by SULT2B1b since expression of neither SULT2A1 nor SULT1E1 was detected in human brain sections. SULT2B1b mRNA and protein were also detected in human U373-MG glioblastoma cells. Both mRNA and protein expression of liver X receptor (LXR)-β, but not LXR-α, were detected in U373-MG cells, and LXR-β activation resulted in a decrease in SULT2B1b protein expression. Since hydroxycholesterols are important physiological LXR activators, this suggests a role for regulation of sterol metabolism by LXR and SULT2B1b. Therefore, elucidating key enzymes in the metabolism of cholesterol and neuro­steroids could help define the properties of steroid conjugation in the human brain.
Alteration Behavior of High Burnup Spent Fuel in Salt Brine Under Hydrogen Overpressure and in Presence of Bromide
Authors: Loida Andreas , Metz Volker , Kienzler Bernhard 
Published: October 2011

AbstractRecent studies have shown that in the presence of H2 overpressure, which forms due to the corrosion of the Fe based container, the dissolution rate of the spent fuel matrix is slowed down by a factor of about 10, associated with a distinct decrease of concentrations of important ra-dionuclides. However, in a natural salt environment as well as in geological formations with chloride rich groundwater the presence of radiation chemically active impurities such as bro-mide must be taken in consideration. Bromide is known to react with β/γ radiolysis products, thus counteracting the protective H2 effect. In the present experiments using high burnup spent fuel it is observed that during 212 days the matrix dissolution rate was enhanced by a factor of about10 in the presence of up to 10-3 M bromide and 3.2 bar H2 overpressure. However, concen-trations of matrix bound actinides were found at the same level or below as found under identical conditions, but in the absence of bromide. In the long-term it is expected that the effect of bro-mide becomes less important, because the decrease of β/γ-activity results in a decrease of oxida-tive radicals, which react with bromide, while α activity will dominate the radiation field.