bluuebunny/crossref_metadata_2025_split huggingface dataset:
NF‐κB activation stimulates osteogenic differentiation of mesenchymal stem cells derived from human adipose tissue by increasing TAZ expression
Authors: Cho Hyun Hwa , Shin Keun Koo , Kim Yeon Jeong , Song Ji Sun , Kim Jong Myung , Bae Yong Chan , Kim Chi Dae , Jung Jin Sup 
Published: January 2010

AbstractTumor necrosis factor‐alpha (TNF‐α) is a skeletal catabolic agent that stimulates osteoclastogenesis and inhibits osteoblast function. Although TNF‐α inhibits the mineralization of osteoblasts, the effect of TNF‐α on mesenchymal stem cells (MSC) is not clear. In this study, we determined the effect of TNF‐α on osteogenic differentiation of stromal cells derived from human adipose tissue (hADSC) and the role of NF‐κB activation on TNF‐α activity. TNF‐α treatment dose‐dependently increased osteogenic differentiation over the first 3 days of treatment. TNF‐α activated ERK and increased NF‐κB promoter activity. PDTC, an NF‐κB inhibitor, blocked the osteogenic differentiation induced by TNF‐α and TLR‐ligands, but U102, an ERK inhibitor, did not. Overexpression of miR‐146a induced the inhibition of IRAK1 expression and inhibited basal and TNF‐α‐ and TLR ligand‐induced osteogenic differentiation. TNF‐α and TLR ligands increased the expression of transcriptional coactivator with PDZ‐binding motif (TAZ), which was inhibited by the addition of PDTC. A ChIP assay showed that p65 was bound to the TAZ promoter. TNF‐α also increased osteogenic differentiation of human gastroepiploic artery smooth muscle cells. Our data indicate that TNF‐α enhances osteogenic differentiation of hADSC via the activation of NF‐κB and a subsequent increase of TAZ expression. J. Cell. Physiol. 223: 168–177, 2010. © 2009 Wiley‐Liss, Inc.
Discovery of a benzoxazine derivative promoting angiogenesis in vitro and in vivo
Authors: Dong ZhiWu , Cheng YiZhe , Zhao Jing , Su Le , Zhao BaoXiang , Zhang Yun , Zhang ShangLi , Miao JunYing 
Published: January 2010

AbstractAngiogenesis is a multi‐step process that refers to the growth of new vessels from pre‐existing ones. Endothelial proliferation, migration, and tube formation constitute a critical step in angiogenesis. Recently, we demonstrated that a novel benzoxazine derivative, 6‐amino‐2,3‐dihydro‐3‐hydroxymethyl‐1,4‐benzoxazine (ABO) could improve the proliferation of human umbilical vein endothelial cells (HUVECs) without basic fibroblast growth factor (bFGF) and serum. In this study, we further tested its effect on endothelial angiogenesis with Matrigel assay, migration assay, and in vivo chick chorioallantoic membrane (CAM) assay. Our results showed that ABO effectively facilitated cell migration and promoted capillary‐like tube formation in vitro and in vivo. To elucidate the underlying mechanisms, we examined intracellular reactive oxygen species (ROS) level/nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and superoxide dismutase (SOD) activities, nitric oxide (NO) level/endothelial nitric oxide synthase (eNOS) activity, and mitochondrial membrane potential (MMP). Our data indicated that ABO depressed ROS with inhibition of NADPH oxidase instead of SOD activity, stimulated NO production and eNOS activation, and restored MMP in HUVECs. Our findings suggest that ABO is a promising tool for exploring the mechanisms of angiogenesis and may have a therapeutic potential in ischemic pathologies. J. Cell. Physiol. 223: 202–208, 2010. © 2009 Wiley‐Liss, Inc.
Membrane lipid defects are responsible for the generation of reactive oxygen species in peripheral blood mononuclear cells from vitiligo patients
Authors: Dell'Anna Maria Lucia , Ottaviani Monica , Bellei Barbara , Albanesi Veronica , Cossarizza Andrea , Rossi Luisa , Picardo Mauro 
Published: January 2010

AbstractThe pathogenesis of vitiligo, an acquired depigmenting disease of the skin, involves oxidative stress. Based on that, the generation of reactive oxygen species (ROS) by the mitochondria may be relevant in the pathogenesis of vitiligo. Here, we evaluate the biochemical and functional alterations involved in the defective activity that has been previously described both in melanocytes and peripheral blood mononuclear cells (PBMC) from vitiligo patients. Moreover, we used a freeze–thaw test as a mild stress stimulus to disclose any latent defects in the assembly of membrane lipids that may compromise the functionality of the membrane itself. We show that the lipid constitution of the membrane is altered in vitiligo. Specifically, the cardiolipin (CL) level in the mitochondrial inner membrane is reduced and the level of cholesterol is increased. Furthermore, an increase in the expression level of 3‐hydroxy‐3methyl‐glutaryl‐CoenzymeA‐reductase (HMG‐CoA reductase), the rate‐limiting enzyme for cholesterol biosynthesis, was also seen. Associated with that, the expression of electron transport chain (ETC) lipid‐dependent subunits was also modified, and their expression was further affected by the freeze–thaw stress. The expression of CL‐independent mitochondrial proteins, such as porin and Bcl2, were unaffected in vitiligo PBMC. These data confirm that ETC protein expression mainly correlates with lipid arrangement and that loss of their expression is not due to generalized or random oxidative‐mediated damage. We suggest that the modification of membrane lipid components in vitiligo cells may be the biochemical basis for the mitochondrial impairment and the subsequent production of intracellular ROS following the exposure to a mild stress. J. Cell. Physiol. 223: 187–193, 2010. © 2009 Wiley‐Liss, Inc.
Voltage‐dependent calcium and chloride currents in S17 bone marrow stromal cell line
Authors: Silva Henrique B. , Medei Emiliano , Rodrigues Deivid C. , Rondinelli Edson , Almeida Norma A.S. , Goldenberg Regina C.S. , de Carvalho Antonio C. Campos , Nascimento José H.M. 
Published: January 2010

AbstractThe bone marrow stromal cell line S17 has been used to study hematopoiesis in vitro. In this study, we demonstrate the presence of calcium and chloride currents in cultured S17 cells. Calcium currents were of low amplitude or barely detectable (50–100 pA). Hence to amplify the currents, we have used barium as a charge carrier. Barium currents were identified based on their distinct voltage‐dependence, and sensitivity to dihydropyridines. S17 cells also exhibited a slowly activating outward current without inactivation, most commonly seen when the sodium of the extracellular solution was replaced either by TEA (TEA/Cs saline) or NMDG (NMDG saline), or by addition of amiloride to the extracellular solution. This current was abolished either by 500 µM SITS (4,4′‐diisothiocyanatostilbene‐2‐2′‐disulfonic acid) or 500 µM DPC (diphenylamine‐2‐carboxylic acid) a cystic fibrosis transmembrane conductance regulator (CFTR) chloride channel blocker, identifying it as a Cl− current. RT‐PCR identified the presence of ENaC and CFTR transcripts. CFTR blockade reduced cell proliferation, suggesting that this channel plays a physiological role in regulation of S17 cell proliferation. J. Cell. Physiol. 223: 244–251, 2010. © 2009 Wiley‐Liss, Inc.
Novel synthesis of [13C4,15N]1H‐pyrrole‐2,3,5‐tricarboxylic acid: an important biomarker for melatonin metabolism
Authors: Skaddan Marc B. 
Published: January 2010

Abstract1H‐pyrrole‐2,3,5‐tricarboxylic acid is a breakdown product of melatonin. A labeled version of this compound would serve as a key biomarker for drug candidates which track this substance to monitor their effectiveness (e.g. hyperpigmentation drugs). A Hantzsch synthesis using readily available starting materials was used to generate [13C4,15N]1H‐pyrrole‐2,3,5‐tricarboxylic acid in six steps (12% overall yield). Copyright © 2009 John Wiley & Sons, Ltd.
Mg2+modulates integrin–extracellular matrix interaction in vascular smooth muscle cells studied by atomic force microscopy
Authors: Trache Andreea , Trzeciakowski Jerome P. , Meininger Gerald A. 
Published: January 2010

AbstractAtomic force microscopy (AFM) was used to investigate the interaction between α5β1 integrin and fibronectin (FN) in the presence of divalent cations. AFM probes were labeled with FN and used to measure binding strength between α5β1 integrin and FN by quantifying the force required to break single FN–integrin bonds on a physiological range of loading rates (100–10 000 pN/s). The force necessary to rupture single α5β1–FN bond increased twofold over the regime of loading rates investigated. Changes in Mg2+and Ca2+concentration affected the thermodynamical parameters of the interaction and modulated the binding energy. These data indicate that the external ionic environment in which vascular smooth muscle cells reside, influences the mechanical parameters that define the interaction between the extracellular matrix and integrins. Thus, in a dynamic mechanical environment such as the vascular wall, thermodynamic binding properties between FN and α5β1 integrin vary in relation to locally applied loads and divalent cations concentrations. These changes can be recorded as direct measurements on live smooth muscle cells by using AFM. Copyright © 2009 John Wiley & Sons, Ltd.
Localization and quantification of noise sources in four‐gate field‐effect‐transistors
Authors: Luque Rodríguez A. , Jiménez Tejada J. A. , Godoy A. , López Villanueva J. A. , Gómez‐Campos F. M. , Rodríguez‐Bolivar S. 
Published: January 2010

AbstractIn this paper the effects of different noise sources in a four‐gate field‐effect‐transistor have been studied and quantified in different operation regimes of the structure. To carry out this study, a model that captures the main features of generation–recombination noise, produced by the fluctuations of trapped charge in the depletion regions of the device, and 1/f noise, produced by the fluctuations of trapped charges at the silicon–oxide interfaces, has been incorporated into a two‐dimensional device simulator. The existence of different kinds of bulk traps in the semiconductor may complicate the interpretation of experimental noise measurements. Anomalies in the noise behavior, produced by certain traps at specific biased voltages, have been explained by means of the simulation of the device. Copyright © 2010 John Wiley & Sons, Ltd.
Accurate prediction of the volume inversion impact on undoped Double Gate MOSFET capacitances
Authors: Moldovan Oana , Chaves Ferney A. , Jiménez David , Raskin Jean‐Pierre , Iñiguez Benjamin 
Published: January 2010

AbstractThis paper demonstrates the capability of our previously published undoped Double‐Gate (DG) MOSFET explicit and analytical compact model to also forecast the effect of the volume inversion (VI) on the intrinsic capacitances. For that purpose, we present simulation results for these capacitances. We show now that the model presents an accurate dependence on the silicon layer thickness, consistent with two‐dimensional numerical simulations, for both thin and thick silicon films. As opposed to our previous work, here we test the capacitance model for three different film thicknesses and also show that the transition from VI regime to dual gate behaviour is well simulated. We demonstrate in this paper that even if the current drive and transconductance are enhanced in VI regime, our results show that intrinsic capacitances are higher as well, which may limit the high‐speed (delay time) behaviour of DG MOSFETs under VI regime. The good agreement between the numerical simulations and our model shows the high potential of our complete DG MOSFET model. Copyright © 2010 John Wiley & Sons, Ltd.
High‐Efficiency Silicon Solar Cells Demand New Process Tools
Authors: Colville Finlay 
Published: January 2010

AbstractLaser‐based tools are becoming an essential part of new crystalline silicon cell lines which are configured for high‐efficiency performance. Different tooling types are required on a case‐by‐case basis, depending on the level of process development undertaken and how fast cell producers move to implement the laserbased tools within high‐throughput production lines.
Light as a Controlling Tool
Authors: Haase Jens 
Published: January 2010

AbstractThe photovoltaic industry is characterized by a permanent, substantial growing during the last years. Today improving efficiencies and reduction of manufacturing cost of solar cells is essential for the success in the competitive market. The reduction of manufacturing costs is associated with high volume manufacturing of the solar cells by perpetuation of high quality standards and requirements for small tolerances. Measurements of the topography of solar cells now start to play an important role in the quality assurance of the manufacturing process. It allows the three‐dimensional mapping of a complete area with subsequent parameter extraction: so the efficiency of a solar cell depends on the wafer structure: Perfect smooth surfaces absorb less photons than surfaces with a certain, optimized roughness, whereas protecting layers should be as smooth and flat as possible.Similar to all Microsystems the structures can be investigated and compared to the target values: examples are layer thickness, widths and depths of structured lines, the volume‐determination of hollows, defects, pores or abrasion/deposition rates. It also encompasses the 3D profile of printed circuit board tracks or special structures for sophisticated high efficiency photovoltaic elements.
A general model for porous medium flow in squeezing film situations
Authors: Nabhani Mohamed , El Khlifi Mohamed , Bou‐Saïd Benyebka 
Published: January 2010

AbstractThe present paper deals with a numerical investigation of the hydrodynamic lubrication of a porous squeeze film between two circular discs. To this purpose, the thin film (reduced) Navier Stokes equations and a generalised porous medium model are solved. The numerical results show that the effect of the porous disc is to reduce the lubricating properties of the fluid film. This effect is increased during the squeezing action. In addition, it is shown that the film pressure, the load‐carrying capacity and the velocity field based only on the Darcy model are predicted higher than those obtained from the generalised porous medium model. Copyright © 2009 John Wiley & Sons, Ltd.
Free flap failure in an anticardiolipin antibody‐positive patient with neoplasm–A case report
Authors: Asai Emiko , Okouchi Masayuki , Momiyama Masanori , Kajikawa Akiyoshi , Ueda Kazuki 
Published: January 2010

AbstractWe present herein a case of massive arterial thrombosis of a free rectus abdominal musculocutaneous flap used for reconstructive surgery of gingival carcinoma that could not be rescued. A 54‐year‐old woman underwent the operation. She had experienced two miscarriages in her 20s, but medical history was otherwise uneventful. Intraoperatively, the anastomosed artery often showed massive arterial thrombosis, and the flaps had become necrotic after bilateral flaps were used. Laboratory findings, 7 days postoperatively, showed high levels of immunoglobulin G anticardiolipin antibody. This value normalized by 2 months postoperatively after using chemotherapy. This case does not match the criteria for antiphospholipid syndrome, but some English‐language reports have shown rising antiphospholipid antibody levels, particularly anticardiolipin antibodies, in patients with neoplasm. In those cases, levels have normalized after successful therapy. Antiphospholipid antibody levels should be examined before surgery to identify risks of hypercoagulability. © 2010 Wiley‐Liss, Inc. Microsurgery, 2010.
Diagnostic assessment and therapeutic approach for immunodeficiency due to chylous dysplasia: A case report
Authors: Boccardo Francesco , Bellini Carlo , Girino Margherita , Campisi Corrado , Vidali Francesca , Corazza Gino Roberto , Campisi Corradino 
Published: January 2010

AbstractAmong possible causes of a condition of immunodeficiency, we have to consider the presence of a serious chylous dysplasia, due to the great loss of proteins through the intestinal lumen. A 20‐year‐old male, suffered from diarrhoea (2–4 times a day), weight loss (8 kilos in 5 years), and malnutrition (hypogammaglobulinemia, hypoalbuminemia, leukocytopenia with lymphocytopenia). Accurate diagnostic assessment allowed to diagnose a protein‐losing enteropathy. Conventional oil contrast lymphangiography allowed to accurately assess the case and to establish a proper therapeutic approach. The operation consisted in multiple antigravitational ligatures of dilated and incompetent chylous vessels and chylous vessel‐mesenteric vein microanastomoses. Parameters concerning albumin and leukocytes normalized in 1 week after operation and remained stable with time; there were no more episodes of diarrhoea and the patient recovered weight. An accurate diagnostic assessment and above all lymphangiography allow to diagnose properly difficult cases of immunodeficiency due to intestinal protido‐dispersion and to plan a correct therapeutic functional approach. © 2010 Wiley‐Liss, Inc. Microsurgery 30:401–404, 2010.
Monoterpenoid glucoindole alkaloids and iridoids from Pterocephalus pinardii
Authors: Gülcemal Derya , Masullo Milena , Alankuş‐Çalışkan Ozgen , Karayıldırım Tamer , Şenol Serdar G. , Piacente Sonia , Bedir Erdal 
Published: January 2010

AbstractA new secondary metabolite, pterocephaline, along with the known cantleyoside, 7α‐morroniside, 3β,5α‐tetrahydrodesoxycordifoline lactam, 5S‐5‐carboxyvincoside, sweroside, and loganin have been isolated from the aerial parts of P. pinardii (Dipsacaceae). Moreover, cantleyoside‐methyl‐hemiacetal and cantleyoside‐dimethyl‐acetal were obtained as seco‐iridoid artifacts. The structures were elucidated by extensive spectroscopic methods including 1D‐(1H, 13C and TOCSY) and 2D‐NMR (DQF‐COSY, HSQC and HMBC). Monoterpenoid glucoindole alkaloids were encountered for the first time in Dipsacaceae family. Copyright © 2010 John Wiley & Sons, Ltd.
Ongoing remission after intensive All‐type chemotherapy in pediatric intestinal T‐cell lymphoma
Authors: Friedrich Carsten , Schrum Johanna , Chott Andreas , Janka‐Schaub Gritta , Kabisch Hartmut 
Published: January 2010

AbstractA rare case of primary intestinal T‐cell lymphoma (ITL) of an 8‐year‐old boy is reported. Medium‐ to large‐sized tumor cells were βF1+, CD3+, CD8+. TIA‐1+, but CD4−, CD5−, CD30−, CD56−, CD20−, CD79a−, TdT−, consistent with an intraepithelial lymphocyte (IEL) origin. They showed monoclonal rearrangement of the T‐cell receptor γ‐chain and no evidence of EBV infection. No clinical, histologic, laboratory, or genetic evidence of celiac disease was detected. In adults, ITL is often associated with enteropathy and has a very poor outcome. Our patient remains in first remission 30 months after finishing the acute lymphoblastic leukemia protocol COALL‐07‐03 high risk standard. Pediatr Blood Cancer 2010;54:610–612. © 2009 Wiley‐Liss, Inc.
Pre‐B‐cell acute lymphoblastic leukemia with bulk extramedullary disease and chromosome 22 (EWSR1) rearrangement masquerading as Ewing sarcoma
Authors: Jakovljević Gordana , Nakić Melita , Rogošić Srđan , Kardum‐Skelin Ika , Mrsić‐Davidović Sanja , Zadro Renata , Krušlin Božo 
Published: January 2010

AbstractWe report a 2‐year‐old female with a subcutaneous tumor who was initially misdiagnosed as suffering from Ewing sarcoma with a positive EWSR1 rearrangement and EWS/FLI1 transcript. After finding lymphoblasts in peripheral blood, the diagnosis of acute lymphoblastic leukemia was established. This necessitated further analysis of the subcutaneous tumor. The tissue was positive for immature B‐cell markers and an immunoglobulin heavy chain gene rearrangement, which confirmed the final diagnosis of common type acute lymphoblastic leukemia with bulk extramedullary disease. The patient was treated with chemotherapy and was in remission 30 months after the diagnosis. Pediatr Blood Cancer 2010;54:606–609. © 2009 Wiley‐Liss, Inc.
Teratoma with a malignant somatic component in pediatric patients: The Associazione Italiana Ematologia Oncologia Pediatrica (AIEOP) experience
Authors: Terenziani Monica , D'Angelo Paolo , Bisogno Gianni , Boldrini Renata , Cecchetto Giovanni , Collini Paola , Conte Massimo , De Laurentis Tina , Ilari Ilaria , Indolfi Paolo , Inserra Alessandro , Piva Luigi , Siracusa Fortunato , Spreafico Filippo , Tamaro Paolo , Lo Curto Margherita 
Published: January 2010

AbstractBackgroundTeratoma with a malignant somatic component (TMSC) is rare but described in adults, whereas information on pediatric presentation is sparse.ProcedureThe Associazione Italiana Ematologia Oncologia Pediatrica identified 14 cases of TMSC. Clinical files and pathology specimens were reviewed.ResultsThe series (9 female, 5 male) showed the following disease: testis (2), sacrococcygeal (3), ovary (3), retroperitoneum (3), mediastinum (2), and foot soft tissue (1). Distribution of the somatic component was: carcinoma (4), pancreatic neuroendocrine tumor (1), neuroblastoma (3), rhabdomyosarcoma (3), rhabdomyosarcoma plus liposarcoma, chondrosarcoma, neurogenic sarcoma (1), chondrosarcoma plus neuroectodermal sarcoma (1), malignant peripheral nerve sheath tumor (1). Three patients were in stage I, four in stage II, three in stage III, and four in stage IV. All but one patient underwent surgery and only females showed carcinoma components. Nine patients relapsed or progressed and eight died. Six patients are alive and disease‐free. Two patients underwent complete resection and four were treated based on transformed histologies. Relapse‐free and overall survival rates were 35.7% and 42.8%, respectively (median follow‐up, 31 months).ConclusionsPrognosis for germ cell tumors (GCTs) containing MSC is worse than that for GCTs. The pediatric disease appears to be more heterogeneous in tumor site distribution and MSC histology than in adults. Our series suggests no effects of age, histology, or gender on outcome. Surgery has an essential role in localized disease, with complete resection highly desirable. Chemotherapy optimized for histology should include reagents directed to the somatic malignancy, if chemosensitive. Malignant GCT warrants GCT‐directed chemotherapy. Pediatr Blood Cancer 2010;54:532–537. © 2009 Wiley‐Liss, Inc.
Diffuse central nervous system protoplasmic astrocytoma
Authors: Manley Sue , Crooks Daniel , Artingstall Libby , Buxton Neil , Appleton Richard , Riordan Andrew , Cleary Gavin , Pizer Barry 
Published: January 2010

AbstractProtoplasmic astrocytoma is an extremely rare form of grade II low grade glioma which usually presents as a discrete mass lesion. We describe a 3‐year‐old female with diffuse protoplasmic astrocytoma with parenchymal involvement and leptomeningeal spread. This tumour proved extremely difficult to diagnose and followed a progressive course. Three superficial biopsies did not give the diagnosis and this was only confirmed 8 months from presentation from a larger fourth biopsy taken deeper from the cerebellum. To our knowledge this case represents the distinct presentation of protoplasmic astrocytoma presenting as extensive diffuse meningeal disease. Pediatr Blood Cancer 2010;54:768–769. © 2009 Wiley‐Liss, Inc.