bluuebunny/crossref_metadata_2025_split huggingface dataset:
Eight new and three recalculated orbits for binaries
Authors: Cvetković Z. , Novaković B. 
Published: March 2010

AbstractIn this paper new orbital elements are given for eleven binaries. For eight of them, WDS 00003–4417 = I 1477, WDS 00106–7313 = I 43 AB, WDS 00366+5609 = A 914, WDS 00519–4343 = I 47, WDS 01315+1521 = BU 506, WDS 01577+4434 = A 1526, WDS 08144–4550 = FIN 113 AB and WDS 08291–4756 = FIN 315 Aa‐Ab, the orbital elements are calculated for the first time. For three of them, WDS 04422+2257 = MCA 16 Aa‐Ab, WDS 08275–5501 = FIN 116 and WDS 14567–6247 = FIN 372, the orbital elements are recalculated. One of the eleven binaries, MCA 16 Aa‐Ab, was discovered by McAlister in 1980 by speckle interferometry and four pairs were discovered by Finsen between 1929 and 1960. For these five pairs, all measured separations are less than 0″.4 and most of the observations were done by using the interferometric techniques. The orbital periods calculated here are between 39 and 270 years. The remaining six pairs were discovered between 1878 and 1926 and most of the observations are visual. They have longer orbital periods, between 384 and 1637 years. In addition to the orbital elements the masses, dynamical parallaxes, absolute magnitudes and ephemerides for the next five years are also given in this paper (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Finding the most variable stars in the Orion Belt with the All Sky Automated Survey
Authors: Caballero J.A. , Cornide M. , Castro E. de 
Published: March 2010

AbstractWe look for high‐amplitude variable young stars in the open clusters and associations of the Orion Belt. We use public data from the ASAS‐3 PhotometricV‐band Catalogue of the All Sky Automated Survey, infrared photometry from the 2MASS and IRAS catalogues, proper motions, and the Aladin sky atlas to obtain a list of the most variable stars in a survey area of side 5° centred on the bright star Alnilam (εOri) in the centre of the Orion Belt. We identify 32 highly variable stars, of which 16 had not been reported to vary before. They are mostly variable young stars and candidates (16) and background giants (8), but there are also field cataclysmic variables, contact binaries, and eclipsing binary candidates. Of the young stars, which typically are active Herbig Ae/Be and T Tauri stars with Hαemission and infrared flux excess, we discover four new variables and confirm the variability status of another two. Some of them belong to the well knownσOrionis cluster. Besides, six of the eight giants are new variables, and three are new periodic variables (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
The young open cluster Trumpler 3
Authors: Maciejewski G. , Bukowiecki L. 
Published: March 2010

AbstractWe present a photometric and spectroscopic study of the poorly investigated open cluster Trumpler 3. Basic parameters such as the age of 70 ± 10 Myr, the color excess E (B – V) = 0.30 ± 0.02 mag, the distance of 0.69 ± 0.03 kpc and the limiting radius of 12′ were redetermined and compared with previous preliminary studies. The distance of 0.65 ± 0.09 kpc was determined independently by spectral parallaxes. Simultaneously, our analysis allowed us to estimate a total number of members to be Ntot = 570 ± 90 and a total mass of the cluster to be Mtot = 270 ± 40 M⊙. We also determined a state of cluster's dynamical evolution. We conclude that Trumpler 3 is a young low‐massive stellar ensemble with a typical mass function slope, located near to the outer edge of the Galaxy's Orion Spur. As a result of a wide‐field search for short period variable stars, 24 variables were discovered in the cluster's area. Only one of them – a variable of the γ ‐Dor type – was found to be a likely cluster member (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Increased Levels of Proinflammatory Cytokines in Children with Family History of Coronary Artery Disease
Authors: E. Lefkou , Fragakis N. , Ioannidou E , Bounda A , Theodoridou S , Klonizakis P , Garipidou V 
Published: March 2010

AbstractBackgroundParental history of coronary artery disease (CAD) is considered an important risk factor for early atherosclerosisHypothesisThe onset of the inflammatory process of atherosclerosis initiates early during childhood in children with positive family history (PFH) of CAD.MethodsWe studied 55 healthy children (5–15 years), 30 (16 male) with PFH and 25 age and sex matched control subjects. Blood samples were taken to measure white blood count (WBC), glucose, total cholesterol, triglycerides (TG), high‐density lipoprotein (HDL), low‐density lipoprotein (LDL), erythrocyte sedimentation rate (SDE), C‐reactive protein (CRP), interleukin‐6 (IL‐6), and tumor necrosis factor‐alpha (TNF‐a). We performed cultures on monocytes (from peripheral blood) measuring in the cell culture supernatants the proinflammatory cytokines IL‐6 and TNF‐a, by using the immunoassay ELISA method.Results:Higher values of body mass index (BMI), total cholesterol, LDL, cholesterol, TG, SDE, leucocytes, and CRP were calculated in children with PFH. Significantly higher values of cytokines in monocell cultures were measured in the PFH group compared to the control group (IL‐6 = 139.32 ± 80.84 pg/ml versus 14.30 ± 12.97 pg/ml, p < 0.001 and TNF‐a = 39.91 ± 11.80 pg/ml versus 8.65 ± 4.35 pg/ml, p < 0.001). IL‐6 values in plasma and cultures were found independently associated with PFH of premature CAD (p < 0.001, p = 0.005, respectively). A similar relation was found for TNF‐a values measured in cultures (p = 0.005) and CRP values in plasma (p < 0.001). The values of IL‐6 were found proportionally related to TG.ConclusionIn individuals with PFH of CAD the inflammatory process of atheromatosis appears to begin early in childhood. Except for triglycerides, this inflammatory process appears to occur independently of several traditional cardiovascular risk factors. Copyright © 2010 Wiley Periodicals, Inc.
Isolation of a Small‐Molecule Inhibitor of the Antiapoptotic Protein Bcl‐xL from a DNA‐Encoded Chemical Library
Authors: Melkko Samu , Mannocci Luca , Dumelin Christoph E. , Villa Alessandra , Sommavilla Roberto , Zhang Yixin , Grütter Markus G. , Keller Nadine , Jermutus Lutz , Jackson Ronald H. , Scheuermann Jörg , Neri Dario 
Published: March 2010

AbstractBcl‐xL is an antiapoptotic member of the Bcl‐2 protein family and an attractive target for the development of anticancer agents. Here we describe the isolation of binders to Bcl‐xL from a DNA‐encoded chemical library using affinity‐capture selections and massively parallel high‐throughput sequencing of >30 000 sequence tags of library members. The most potent binder identified, compound 19/93 [(R)‐3‐(amido indomethacin)‐4‐(naphthalen‐1‐yl)butanoic acid], bound to Bcl‐xL with a dissociation constant (Kd) of 930 nM and was able to compete with a Bak‐derived BH3 peptide, an antagonist of Bcl‐xL function.
Development and application of a multiplexable flow cytometry‐based assay to quantify cell‐mediated cytolysis
Authors: Cao Lan‐Feng , Krymskaya Ludmila , Tran Vivi , Mi Shu , Jensen Michael C. , Blanchard Suzette , Kalos Michael 
Published: March 2010

AbstractAlthough target cell cytolysis has been widely employed to describe effector function of cells, cytolysis assays as commonly employed do not generate quantitative data. In this report we describe the development and application of a statistically supported flow cytometry‐based assay to quantify cell‐mediated cytolysis. The assay depends on the use of the fluorescent dye CFSE to distinguish target from effector cells, the DNA intercalating dye 7AAD to distinguish dead from live cell events, and on the establishment of a cytolysis curve that allows for the derivation of statistically robust data. We demonstrate that the cytolysis curve is well described by a four parameter logistic regression model provided that (i) the range of effector to target (E:T) ratios studied allows for full description of the logistic curve, and (ii) an adequate number of data points are collected to estimate the model parameters. We show that the assay is highly reproducible and accurate, and comparable in sensitivity with the standard 51Cr assay. We report on the potential for this assay to generate quantitative data on the cytolytic activity of both CD8 T and NK cells; describe a relationship between the efficiency of effector cell degranulation and target cell cytolysis throughout a range of E:T ratios, and demonstrate the potential to multiplex with other platforms to obtain broader datasets for the effector phenotype of cells. Appropriate use of this assay will enhance the ability to derive quantitative and integrated correlative datasets from basic, translational, and clinical studies. © 2010 International Society for Advancement of Cytometry
Comparison of platelet counts by CellDyn Sapphire (Abbot Diagnostics), LH750 (Beckman Coulter), ReaPanThrombo immunoplatelet method (ReaMetrix), and the international flow reference method, in thrombocytopenic blood samples
Authors: Sehgal Kunal , Badrinath Y. , Tembhare Prashant , Subramanian P. G. , Talole Sanjay , Kumar Ashok , Gadage Vijaya , Mahadik Shashikant , Ghogale Sitaram , Gujral Sumeet 
Published: March 2010

AbstractBackground:We compared the international flow reference method (IRM) platelet counts with those obtained from CellDyn Sapphire (impedance and optical counts), LH750 (impedance counts), and the flowcytometry based ReaPanThrombo Immunoplatelet method (ReaMetrix). We further evaluated the degree of agreement of above methods with the IRM at the transfusion thresholds of 10 × 109l−1and 20 × 109l−1.Methods:A total of 104 thrombocytopenic blood samples with platelet count of <50 × 109l−1were selected for the study. All samples were tested in parallel by various methods within 6 h of blood collection.Results:For bias estimation, a Bland–Altman analysis was done by taking the IRM as the standard method. The bias for CDS‐I counts was +6.505 × 109l−1(95% LA −2.110 to +15.122), for CDS‐O counts the bias was −3.779 × 109l−1(95% LA −8.950 to +1.392), for LH750 the bias was +0.111 × 109l−1(95% LA −5.862 to +6.084) and that for ReaMetrix was −1.602 × 109l−1(95% LA −7.400 to +4.194). The LH750 had the least average bias and it overestimated platelet counts marginally. The ReaMetrix method showed the highest degree of agreement with the IRM, at both the threshold points with aKvalue of 0.960 (threshold ≤ 10 × 109l−1) and 0.923 (threshold ≤ 20 × 109l−1).Conclusions:Impedance platelet counts from LH750 were more accurate than optical methods in thrombocytopenic patients. ReaMetrix immunoplatelet counts show the maximum degree of agreement with the IRM at clinically relevant transfusion thresholds. We conclude that as current platelet transfusion thresholds are based on results of automated hematology analyzer methods, the true thresholds may be determined using the IRM and CD41/61 based single‐platform immunoplatelet methods. © 2010 Clinical Cytometry Society
Extraction of Nitraria tangutorum seed lipid using different extraction methods and analysis of its fatty acids by HPLC fluorescence detection and on‐line MS identification
Authors: Suo Yourui , Wang Lingyun 
Published: March 2010

AbstractThe seed lipid of Nitraria tangutorum was extracted by supercritical carbon dioxide extraction, microwave‐assisted reflux extraction, ultrasound‐assisted extraction, or solvent reflux extraction. The experimental parameters of supercritical carbon dioxide extraction including pressure, temperature, particle size, and extraction time were investigated. A facile and sensitive method for the simultaneous determination of 30 saturated and 9 unsaturated fatty acids by HPLC with fluorescence detection after pre‐column derivatization was developed. Fatty acid derivatives were separated on a reversed‐phase Eclipse XDB‐C8 column in conjunction with gradient elution. Identification of fatty acid derivatives was carried out by on‐line APCI/MS in positive‐ion mode. Excellent quantitative linear responses of the 39 fatty acids were observed in the range of 0.014 to 14 μmol/L with correlation coefficients higher than 0.9992. Limits of detection were in the range of 0.32–3.7 nmol/L (S/N = 3). The fatty acids in Nitraria tangutorum seed lipid with or without saponification extracted by the four different methods were determined and compared. The results indicated that the mass percentage of unsaturated fatty acids (mainly oleic acid, linoleic acid and linolenic acid) in Nitraria tangutorum seed lipid was up to 79%, and the best method was supercritical carbon dioxide extraction.
Policosanol characterization and accumulation during ripening of Tunisian Olea europaea L. fruits
Authors: Sakouhi Faouzi , Boukhchina Sadok , Absalon Christelle , Fouquet Eric , Kallel Habib 
Published: March 2010

AbstractPolicosanol is a mixture of bioactive molecules shown to have beneficial effects in treating hypercholesterolemia. Food products enriched in policosanol are currently available in the US market. In the present study, eight policosanol components were identified by GC‐MS during the ripening of Meski olives. The quantitative characterization of these compounds was performed using GC‐FID. The results showed that the maximum level of total policosanol components (947.20 mg/100 g oil) was reached at the 26th week after the flowering date of Meski olives. Hexacosanol and tetracosanol were the predominant policosanol components at Meski olive maturity. However pentacosanol, heptacosanol and tricosanol were less present in the olives and they accounted for 14% of the total policosanol at complete maturity of the fruit. The total policosanol content of Meski olives was higher than that of beeswax and whole sugar cane, which belong to the sources of dietary supplements containing policosanol. These findings indicate that olive is a potential source of these health‐enhancing compounds for functional foods and nutraceutical applications.
Effect of triacylglycerol species on the crystallizing behavior of a model water/oil emulsion
Authors: Tanaka Leo , Isogai Tomoyuki , Miura Susumu , Murakami Mototake 
Published: March 2010

AbstractThe crystallizing behavior of a model water/oil (W/O) emulsion with different fat formulas was investigated. The model W/O emulsion was stored in a programmable oven under a temperature fluctuation cycle of 5 °C for 12 h and 20 °C for another 12 h. Crystal growth was observed using a polarization microscope, until the crystals were over 100 μm in diameter, which causes texture degradation. We examined whether the texture degradation is related to the fatty acid formula and the triglyceride formula by carbon number. We also examined the effect of the triglyceride species concentration estimated from the fatty acid formula on the texture degradation. The palmitic acid content was related with texture degradation at high concentration among the fatty acid species. The triglyceride content was not related with texture degradation. Triacylglycerol species with palmitic acid such as tripalmitate (PPP) and 1,3‐dipalmitoyl‐2‐oleoyl‐glycerol (POP) were related with texture degradation. The summed up concentration of three triglycerides [PPP, POP and 1,2‐dipalmitoyl‐3‐oleoyl‐glycerol (PPO)] was related with texture degradation.
Sox10 is necessary for oligodendrocyte survival following axon wrapping
Authors: Takada Norio , Kucenas Sarah , Appel Bruce 
Published: March 2010

AbstractCells of the oligodendrocyte lineage, which form the myelinating glia of the vertebrate central nervous system, undergo a stepwise developmental progression entailing specification from neuroepithelial precursors, proliferation, migration to expand and distribute the population, and differentiation to ensheath axons with myelin. Understanding the genetic mechanisms that regulate each of these steps during development is important, because this might lead to therapies to promote remyelination following neural injury or disease. Genetic studies in mice indicated that the Sox10 transcription factor is required during the differentiation stage to promote myelin gene expression. However, whether Sox10 also promotes other features of oligodendroctye differentiation remained unknown. In this study, we used time‐lapse imaging to investigate the behavior and fates of oligodendrocyte lineage cells in zebrafish embryos and larvae that lacked Sox10 function. This revealed that the myelinating subset of oligodendrocyte progenitor cells (OPCs) migrates, divides, and wraps axons normally, but then dies. Nonmyelinating oligodendrocyte progenitors divided more frequently, maintaining a normal population size. New oligodendrocytes produced by these progenitors wrapped axons and survived, but did not express myelin genes at high levels. We conclude that, in addition to promoting myelin gene expression, Sox10 function is necessary for the survival of myelinating oligodedrocytes subsequent to axon wrapping but is not required for the survival of nonmyelinating OPCs. © 2010 Wiley‐Liss, Inc.
Holocene laminated sediments from the southern Gulf of California: geochemical, mineral magnetic and microfossil study
Authors: Pérez‐Cruz Ligia , Urrutia‐Fucugauchi Jaime 
Published: March 2010

AbstractGeochemical, magnetic mineral analyses and microfossils (radiolarians) are used to characterise a Holocene laminated sequence from the Alfonso Basin, northern Bay of La Paz, southern Gulf of California. In most cases, dark and light laminae show similar bulk chemical compositions and magnetic mineral properties. However, dark laminae contain relatively more terrigenous elements (Al, Si, Fe, K and Mg) supporting a pluvial origin related to the volcanic siliceous tuffs around the Bay of La Paz. In light laminae, calcium content is higher, representing biogenic input. The magnetic signal is dominated by low coercivity, fine‐grained low‐titanium titanomagnetites and magnetite. Hysteresis ratio parameters in domain state plots show that samples fall in the pseudo‐single domain field, indicating mixtures of single‐ and multi‐domain particles. Variations in hysteresis saturation magnetisation parameters for dark and light laminae reflect changes in relative mineral concentration. Results indicate a dominant volcanic source for magnetic minerals into the basin, associated with variable pluvial and/or aeolian transport. Low radiolarian abundance in dark laminae seems to be associated with larger penetration and longer residence time of oligotrophic subtropical waters into the bay that occurred during El Niño–Southern Oscillation (ENSO) events. Light laminae are the result of episodic pulses of high productivity. We suggest that dark/light laminae result from oceanographic and climatic cyclic processes, with main terrigenous input variation possibly associated with ENSO forcing of pluvial influx, and/or latitudinal migration of the Intertropical Convergence Zone. The laminated sequence of the Alfonso Basin formed a non‐annual depositional system, in contrast to the varved sediments characteristic of the central Gulf of California basins. Copyright © 2010 John Wiley & Sons, Ltd.
Tracing growing degree‐day changes in the cuticle morphology of Betula nana leaves: a new micro‐phenological palaeo‐proxy
Authors: Wagner‐Cremer Friederike , Finsinger Walter , Moberg Anders 
Published: March 2010

AbstractChanging growing‐season properties in the northern latitudes are among the most obvious consequences of ongoing global change. Available techniques including satellite monitoring and phenological observations enable the detection of changes over the last few decades to centuries, but the full range of natural variability is still difficult to capture. Here we introduce a new approach to reconstruct growing season properties, by studying imprints of prolonged growing season on epidermal cell growth in Betula nana. A high correlation between cell expansion determined in annually collected B. nana leaves and subfossil leaf fragments collected from recent peat sections in northern Scandinavia, and climatic indices such as budburst date, growing‐season degree‐days and May–September mean temperatures, enable the establishment of a new micro‐phenological proxy for growing season characteristics. The applicability of the epidermal cell undulation index (UI) is tested by comparison with historical instrumental records of growing‐season degree‐days for the last 200 a. The results demonstrate the potential of the new leaf‐morphology‐based technique to reconstruct and quantify past changes in growing degree‐days beyond instrumental data series. Applied to abundant B. nana leaf remains from peat and lake sediments, the UI may enable a reconstruction of growing degree‐days throughout the Holocene and other parts of the late Quaternary. Copyright © 2010 John Wiley & Sons, Ltd.
Surgical anatomy of cartilaginous structures of the Asian nose: Clinical implications in rhinoplasty
Authors: Kim Chang‐Hoon , Jung Dong Hak , Park Mi‐Na , Yoon Joo‐Heon 
Published: March 2010

AbstractObjectives/Hypothesis:The morphologic features of upper and lower lateral cartilage and septal cartilage of the cadaveric nose were analyzed to provide practical anatomical knowledge for Asian rhinoplasty.Study Design:Cadaveric dissection.Methods:A total of 21 Korean adult cadavers were dissected. External nasal morphology was observed, measured, photographed, and analyzed. Histologic features were observed with a light microscope in coronally‐transected specimens stained with hematoxylin and eosin.Results:The lengths of the upper and lower lateral cartilage of Korean cadaveric noses were similar to those of white noses. The widths of the upper and lower lateral cartilage were substantially smaller in Korean cadaveric noses than in those of whites. Upper lateral cartilage include substantial transverse portions near the keystone area that should be preserved in component reduction rhinoplasty. The relationships between the upper lateral cartilage and the lower lateral cartilage were divided into four types. Type I, in which the upper lateral cartilage and lower lateral cartilage are interlocked to form a Z‐shape, is the most common. The posterior portion of the septal cartilage, which is connected to the perpendicular plate of the ethmoid and vomer, is thickest.Conclusions:Cartilaginous structures of Asian noses were substantially different from those of whites in terms of their shape, size, thickness, and relationship with other structures. The data from surgical anatomical observations of the cartilaginous framework of Korean cadaveric noses provided in this report will provide valuable information for performing rhinoplasty on Asian patients. Laryngoscope, 2010
Fundamentals of RAFT Miniemulsion Polymerization Kinetics
Authors: Tobita Hidetaka 
Published: March 2010

AbstractSummary: The polymerization rate of RAFT‐mediated miniemulsion polymerization, in which the time fraction of active radical ϕA is larger than a few percent, basically increases with reducing the particle size. For smaller particle sizes, however, the statistical variation of monomer concentration among particles may slow down the polymerization rate. The rate retardation by increasing the RAFT concentration occurs with or without the intermediate termination in a zero‐one system. According to the present theoretical investigation, smaller particles are advantageous in implementing a faster polymerization rate, a narrower MWD, and a smaller number of dead polymer chains.