bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti-Bovine Viral Diarrhoea Virus Activity of Novel Diphenylmethane Derivatives
Authors: Salim Mohammed TA , Okamoto Mika , Hosoda Shinnosuke , Aoyama Hiroshi , Hashimoto Yuichi , Baba Masanori 
Published: April 2010

Background: A number of compounds were examined for their inhibitory effects on bovine viral diarrhoea virus (BVDV), a surrogate model of hepatitis C virus, in cell cultures. Among them, some diphenylmethane derivatives were found to be selective inhibitors of BVDV. Methods: Determination of compounds for their anti-BVDV activity was based on the inhibition of virus-induced cytopathic effect in Madin–Darby bovine kidney cells and reduction of infectious virus particles in culture supernatants. To gain insight into the mechanism of action, the inhibition of viral entry and RNA synthesis in the host cells was also determined by real-time reverse transcription-PCR. Results: Among the test compounds, four diphenylmethane derivatives significantly inhibited BVDV replication with a 50% effective concentration ranging between 6.3 and 10.8 μM. They were not cytotoxic at concentrations up to 100 μM. The representative compound, SH-595A, reduced the virus titre of culture supernatants in a dose-dependent manner. In addition, the compound appeared to somewhat affect viral entry to the host cells. Although SH-595A was inhibitory to viral RNA synthesis, the inhibition was achieved only at high concentrations and was not comparable to its antiviral activity. Conclusions: The novel diphenylmethane derivatives are effective against BVDV replication and might have a unique mechanism of action.
Activation of Murine Macrophages via TLR2 and TLR4 Is Negatively Regulated by a Lyn/PI3K Module and Promoted by SHIP1
Authors: Keck Simone , Freudenberg Marina , Huber Michael 
Published: April 2010

AbstractSrc family kinases are involved in a plethora of aspects of cellular signaling. We demonstrate in this study that the Src family kinase Lyn negatively regulates TLR signaling in murine bone marrow-derived macrophages (BMMΦs) and in vivo. LPS-stimulated Lyn−/− BMMΦs produced significantly more IL-6, TNF-α, and IFN-α/β compared with wild type (WT) BMMΦs, suggesting that Lyn is able to control both MyD88- and TRIF-dependent signaling pathways downstream of TLR4. CD14 was not involved in this type of regulation. Moreover, Lyn attenuated proinflammatory cytokine production in BMMΦs in response to the TLR2 ligand FSL-1, but not to ligands for TLR3 (dsRNA) or TLR9 (CpG 1668). In agreement with these in vitro experiments, Lyn-deficient mice produced higher amounts of proinflammatory cytokines than did WT mice after i. v. injection of LPS or FSL-1. Although Lyn clearly acted as a negative regulator downstream of TLR4 and TLR2, it did not, different from what was proposed previously, prevent the induction of LPS tolerance. Stimulation with a low dose of LPS resulted in reduced production of proinflammatory cytokines after subsequent stimulation with a high dose of LPS in both WT and Lyn−/− BMMΦs, as well as in vivo. Mechanistically, Lyn interacted with PI3K; in correlation, PI3K inhibition resulted in increased LPS-triggered cytokine production. In this line, SHIP1−/− BMMΦs, exerting enhanced PI3K-pathway activation, produced fewer cytokines than did WT BMMΦs. The data suggest that the Lyn-mediated negative regulation of TLR signaling proceeds, at least in part, via PI3K.
The Small Heat Shock Protein 27 Is a Key Regulator of CD8+CD57+ Lymphocyte Survival
Authors: Wood Karen L. , Voss Oliver H. , Huang Qin , Parihar Arti , Mehta Neeraj , Batra Sanjay , Doseff Andrea I. 
Published: April 2010

Abstract
Differences in CD8+CD57− and CD8+CD57+ lymphocyte lifespan have been documented. Lower numbers and shorter lifespan are characteristic of CD8+CD57+ in normal individuals. However, CD8+CD57+ are expanded in certain disease states including T cell large granular leukemia and other hematologic malignancies. The mechanisms responsible for the differences in CD8+CD57− and CD8+CD57+ lifespan remain elusive. In this study, we demonstrate that the small heat shock protein (Hsp) 27 is a key regulator of CD8+CD57+ lymphocyte lifespan. We found that Hsp27 expression is significantly lower in CD8+CD57+ than in CD8+CD57− lymphocytes. In contrast, Hsp60 and Hsp70 are expressed at comparable levels. Unlike other antiapoptotic Bcl-2–like molecules, the expression of Hsp27 tightly correlates with CD8+CD57+ and CD8+CD57− lifespan. We demonstrate that Hsp27 overexpression in CD8+CD57+ lymphocytes to levels found normally in CD8+CD57− lymphocytes decreased apoptosis. Accordingly, silencing of Hsp27 in CD8+CD57− lymphocytes increased apoptosis. Collectively these results demonstrate that Hsp27 is a critical regulator of normal CD8+CD57+ lifespan supporting its use as a marker of lifespan in this lineage, and suggest a mechanism responsible for the decreased apoptosis and clonal expansion characteristic of certain disease states.
Risk factors associated with renal lithiasis during uricosuric treatment of hyperuricemia in patients with Gout
Authors: Perez‐Ruiz Fernando , Hernandez‐Baldizon Samuel , Herrero‐Beites Ana M. , Gonzalez‐Gay Miguel A. 
Published: April 2010

AbstractObjectiveTo find factors associated with the development of renal colic during uricosuric therapy.MethodsWe performed a prospective cohort followup study of patients with gout and no previous history of kidney stones who had been treated with uricosurics. Clearance of creatinine and urate, 24‐hour urinary uric acid (UA), undissociated urinary UA concentration, 24‐hour undissociated urinary UA, and pH and urine sediment were obtained. Cox proportional hazards regression analysis was used to identify variables associated with renal colic as the outcome. The rate of renal colic was compared with that of control patients receiving allopurinol who had no previous history of renal stones.ResultsWe analyzed a 784 patient‐year exposure from 216 patients: 206 with renal underexcretion of UA and 10 with normal excretion. There were 21 clinical events. Two variables showed increased risk hazard for developing lithiasis: clearance of UA at baseline and undissociated urinary UA concentration during followup. When only patients with underexcretion of UA were included in the analysis, undissociated urinary UA during followup remained the only statistically significant variable. Patients who showed an undissociated UA concentration <20 mg/dl did not show an increase in the rate of lithiasis or events compared with patients receiving allopurinol.ConclusionClearance of UA at baseline may be useful for selecting patients suitable for uricosuric treatment. The estimation of the concentration of undissociated urinary UA is useful for evaluating the risk of lithiasis during followup.
New insights on mass transfer kinetics in chromatography
Authors: Gritti Fabrice , Guiochon Georges 
Published: April 2010

AbstractThe mass transfer kinetics of thiourea, phenol, ethylbenzene, propylbenzene, butylbenzene, and amylbenzene were studied on a Gemini‐C18 (5 μm, 110 A˚, 375 m2/g) column (150 mm × 4.6 mm) eluted with methanol/water solutions (100, 90, and 20% v/v). Each of the successive steps of the mass transfer of these solutes (axial diffusion, eddy dispersion, film mass transfer resistance, and transparticle mass transfer resistance) was unambiguously measured, using a combination of the peak parking method, the total pore blocking method, and moment analysis, in a wide range of reduced linear velocities. The results obtained offer new insights on the mass transfer kinetics in chromatographic columns. They show first that the eddy dispersion A‐term is strongly correlated with the particle porosity. The complex, anastomosed transcolumn flow pattern causes extra band broadening. This transcolumn effect was found to be markedly smaller with porous particles than with nonporous particles of the same size. Second, the film mass transfer coefficient of retained compounds is smaller for porous than for nonporous particles, a result consistent with concentration gradients being steeper at the wall of solid particles than across the entrance surface of pores. The external mass transfer coefficient decreases with increasing fraction of the surface area of the particles that is open to pores, e.g., with increasing particle porosity. © 2010 American Institute of Chemical Engineers AIChE J, 2011
Probability bounds analysis for nonlinear dynamic process models
Authors: Enszer Joshua A. , Lin Youdong , Ferson Scott , Corliss George F. , Stadtherr Mark A. 
Published: April 2010

AbstractDynamic process models frequently involve uncertain parameters and inputs. Propagating these uncertainties rigorously through a mathematical model to determine their effect on system states and outputs is a challenging problem. In this work, we describe a new approach, based on the use of Taylor model methods, for the rigorous propagation of uncertainties through nonlinear systems of ordinary differential equations (ODEs). We concentrate on uncertainties whose distribution is not known precisely, but can be bounded by a probability box (p‐box), and show how to use p‐boxes in the context of Taylor models. This allows us to obtain p‐box representations of the uncertainties in the state variable outputs of a nonlinear ODE model. Examples having two to three uncertain parameters or initial states and focused on reaction process dynamics are used to demonstrate the potential of this approach. Using this method, rigorous probability bounds can be determined at a computational cost that is significantly less than that required by Monte Carlo analysis. © 2010 American Institute of Chemical Engineers AIChE J, 2011
Adjustable QSPRs for prediction of properties of long‐chain substances
Authors: Paster Inga , Shacham Mordechai , Brauner Neima 
Published: April 2010

AbstractThe development of quantitative structure property relationships (QSPRs) with good extrapolation capabilities for high carbon number (nC) substances in homologous series is considered. Based on the available experimental data, molecular descriptors collinear with a particular property are identified. Among these, the ones whose behavior at the limit nC → ∞ is similar to the properties behavior, are eventually used for prediction. A linear QSPR in terms of the selected descriptor with an optional additional correction term which exponentially decays with nC can be developed. The confidence level in the property prediction can be adjusted to the quantity, precision, and reliability of the available data. The proposed method has been tested by developing QSPRs for predicting TC and PC for several homologous series and Tm for the n‐alkane series. In all cases, the QSPRs developed represent the available experimental data satisfactorily and converge to theoretically accepted values for nC → ∞. © 2010 American Institute of Chemical Engineers AIChE J, 2011
Importance of sample intraparticle diffusivity in investigations of the mass transfer mechanism in liquid chromatography
Authors: Gritti Fabrice , Guiochon Georges 
Published: April 2010

AbstractThe effective diffusivity of a nonretained (thiourea) and of a strongly retained (phenol) compounds were measured with the peak parking method in two different columns (both 150 × 4.6 mm) packed with two types of porous particles having different mesopore sizes [5 μm Jupiter‐C18, 320 Å and Luna(2)‐C18, 100 Å]. The eluent was a methanol–water mixture (10/90 v/v) and the temperature 294 K. The effective diffusivity data acquired were used to determine the intraparticle diffusivity, Dp, based on two different diffusion models. The first one assumes that the diffusion fluxes across the particles and in the interparticle volume are additive (parallel diffusion model). The second model was rigorously derived on the basis of the effective medium theory of diffusion (diffusion model) in a binary composite medium (particles + interparticle volume). In both models, it was assumed that the rate of equilibrium between the liquid and the solid phases was infinitely faster than the rate of axial diffusion along the column at zero flow rate. Both models provide physically meaningful intraparticle diffusivity coefficients that take into account the average mesopore size of the particles, their specific surface area, and the retention factor of the analyte. Although the actual effective intraparticle diffusivity remains unknown, these result confirm that the mass transfer resistance due to diffusion through the porous particles has almost negligible effects in reversed phase liquid chromatography due to the importance of surface diffusion. Combining the results of the peak parking method with the h data measured at high linear velocities allows the unambiguous measurement of the film mass transfer and the surface diffusion coefficients. © 2010 American Institute of Chemical Engineers AIChE J, 2011
Quantum chemical studies on the simultaneous interaction of thiophene and pyridine with ionic liquid
Authors: Anantharaj R. , Banerjee Tamal 
Published: April 2010

AbstractThe simultaneous interaction of thiophene and pyridine with different ionic liquids:1‐butyl‐1‐methylpyrrolidinium tetrafluoroborate([BPYRO][BF4]),1‐butyl‐1‐methylpyrrolidinium hexafluoro‐phosphate ([BPYRO][PF6]), 1‐butyl‐4‐methylpyridinium tetrafluoroborate ([BPY][BF4]), 1‐butyl‐4‐methylpyridinium hexafluorophosphate ([BPY][PF6]) and 1‐benzyl‐3‐methylimidazolium tetrafluoroborate ([BeMIM][BF4]) were investigated using quantum chemical calculations. A three‐tier approach comprising of partial charges, interaction energies and sigma profile generation using conductor‐like screening model for real solvents (COSMO‐RS) was chosen to study the systems. A quantitative attempt based on the CH‐π interaction in ionic liquid; thiophene–pyridine complexes gave the interaction energies of ILs in the order: [BPY][BF4] > [BPYRO][PF6] > [BeMIM][BF4] > [BPY][PF6] > [BPYRO][BF4]. An inverse relation was observed between the activity coefficient at infinite dilution predicted via COSMO‐RS–based model and interaction energies. The dominance of CH‐π interaction was evident from the sigma profiles of ionic liquid together with thiophene and pyridine. © 2010 American Institute of Chemical Engineers AIChE J, 2011
Normal ranges of angiogenesis regulatory proteins in human platelets
Authors: Peterson Jon E. , Zurakowski David , Italiano Joseph E. , Michel Lea V. , Fox Lucius , Klement Giannoula L. , Folkman Judah 
Published: April 2010

AbstractPlatelets sequester angiogenesis regulatory proteins early in tumor growth, which suggests a new avenue for monitoring disease. To date, there are no clinically relevant reference ranges for markers of early angiogenesis. We introduce a new ELISA‐based method for accurate and reproducible measurement of vascular endothelial growth factor (VEGF), platelet‐derived growth factor (PDGF), platelet factor 4 (PF4), thrombospondin‐1 (TSP‐1), fibroblast growth factor, basic (bFGF), and endostatin in platelets. To facilitate clinical applicability, the platelet levels in isolated samples were determined utilizing a new actin ELISA method. Platelets from healthy donors at single and repetitive time points were used for the assessment of normal ranges of these proteins. The physiological levels in platelets were: VEGF (0.74 ± 0.37 pg/106 platelets); PDGF (23 ± 6 pg/106); PF4 (12 ± 5 ng/106); TSP‐1 (31 ± 12 ng/106); bFGF (0.44 ± 0.15 pg/106); and endostatin (5.6 ± 3.0 pg/106). There was an excellent correlation (R2 = 0.7) between the platelet levels calculated with the actin ELISA and complete blood count. The levels of the platelets were higher than those in platelet‐poor plasma by factors of: VEGF (215‐fold); PDGF (914‐fold); PF‐4 (516‐fold); TSP‐1 (813‐fold); and bFGF (17‐fold). The endostatin levels were nearly equivalent. The biovariability of the platelet proteins in eight healthy subjects over a 5‐week period was found to be minimal. We describe accurate and direct measurements of the concentrations of VEGF, bFGF, PDGF, TSP‐1, endostatin, and PF4 in platelets of healthy human subjects. In contrast to the highly variable levels in plasma and serum, the platelet‐derived measurements were accurate and reproducible with minimal biovariability. Am. J. Hematol., 2010. © 2010 Wiley‐Liss, Inc.
Comparison of characteristics from White‐ and Black‐Americans with venous thromboembolism: A cross‐sectional study
Authors: Heit John A. , Beckman Michele G. , Bockenstedt Paula L. , Grant Althea M. , Key Nigel S. , Kulkarni Roshni , Manco‐Johnson Marilyn J. , Moll Stephan , Ortel Thomas L. , Philipp Claire S. 
Published: April 2010

AbstractWhen compared with Whites, Black‐Americans may have a 40% higher incidence venous thromboembolism (VTE) incidence. However, whether other VTE characteristics and risk factors vary by race is uncertain. To compare demographic and baseline characteristics among White‐ and Black‐Americans with VTE, we used data prospectively collected from consecutive consenting adults enrolled in seven Centers for Disease Control (CDC) Thrombosis and Hemostasis Centers from August 2003 to March 2009. These characteristics were compared among Whites (n = 2002) and Blacks (n = 395) with objectively diagnosed VTE, both overall, and by age and gender. When compared with Whites, Blacks had a significantly higher proportion with pulmonary embolism (PE), including idiopathic PE among Black women, and a significantly higher proportion of Blacks were women. Blacks had a significantly higher mean BMI and a significantly lower proportion with recent surgery, trauma or infection, family history of VTE, and documented thrombophilia (solely from reduced factor V Leiden and prothrombin G20210A prevalence). Conversely, Blacks had a significantly higher proportion with hypertension, diabetes mellitus, chronic renal disease and dialysis, HIV, and sickle cell disease. When compared with White women, Black women had a significantly lower proportion with recent oral contraceptive use or hormone therapy. We conclude that Whites and Blacks differ significantly regarding demographic and baseline characteristics that may be risk factors for VTE. The prevalence of transient VTE risk factors and idiopathic VTE among Blacks appears to be lower and higher, respectively, suggesting that heritability may be important in the etiology of VTE among Black‐Americans. Am. J.
Hematol. 85:467–471, 2010 © 2010 Wiley‐Liss, Inc.
Molecular characterization of sickle cell anemia in the Northern Brazilian state of Pará
Authors: De Lemos Cardoso Greice , Guerreiro João Farias 
Published: April 2010

AbstractTo assess α+‐thalassemia deletion alleles, β‐thalassemia mutations and haplotypes linked to the HBB*S cluster in a sample of 130 unrelated sickle cell anemia (SCA) patients (55% female) from Belém, Pará State, for their possible effects on the patients' survival. ‐α3.7, ‐α42, ‐α20.5, and –MED α+‐thalassemia deletion alleles were investigated using multiplex gap‐PCR method. Characterization of β‐thalassemia mutations was made by direct genomic sequencing of the β‐globin gene amplified through polymerase chain reaction (PCR). Haplotypes were determined by analysis of six polymorphic restriction sites [(1) XmnI‐5′γG, (2) HindIII‐γG, (3) HindIII‐γA, (4) HincII‐ψβ, (5) HincII‐3′ψβ, and (6) HinfI‐5′β] followed by restriction digestion and agarose gel electrophoresis. Twenty‐one patients (16%) presented ‐α3.7 thalassemia. Sixteen of those (76%) were heterozygous (‐α3.7/αα) and 5 (24%) were homozygous (‐α3.7/‐α3.7). ‐α4.2, ‐α20.5 and –MED deletions were not found. Nine cases of sickle cell‐β thalassemia were found and four different β‐thal mutations were identified: β+ −88 (C>T), 3.8%; β+ codon 24 (T > A), 1.5%; β+ IVSI‐110 (G > A), 0.7% and β (IVSI‐1 (G > A), 0.7%. No differences according to age were observed in ‐α3.7 deletion, β‐thalassemia and HHB*S haplotypes distribution. Our results suggest that although α‐ and β‐thalassemia and βS haplotypes may have modulating effect on clinical expression and hematological parameters of SCA, these genetic variables probably have little influence on the subjects' survival. Am. J. Hum. Biol. 22:573–577, 2010. © 2010 Wiley‐Liss, Inc.
The need for data harmonization—a response to Boden and Ozonoff
Authors: Oleinick Arthur , Zaidman Brian 
Published: April 2010

AbstractBoden and Ozonoff's undercount estimates in their recent Commentary rely on three assumptions for which no quantitative literature references are provided. Alternatively, we show that findings in both studies and published data indicate lower upper‐bound estimates for the undercount range. Am. J. Ind. Med. 53:854–855, 2010. © 2010 Wiley‐Liss, Inc.
Management practices and risk of occupational blood exposure in U.S. paramedics: Needlesticks
Authors: Leiss Jack K. 
Published: April 2010

AbstractBackgroundThe purpose of this study was to present risk estimates for needlestick in U.S. paramedics and estimated risk ratios for selected management practices.MethodsA mail survey was conducted among a national sample of U.S. paramedics in 2002–2003.ResultsThe adjusted response rate was 55% (n = 2,664). The overall 12‐month risk of needlestick was 6.7% (95% confidence interval, 5.4–7.9). Risk ratios for provision of safety‐engineered medical devices and two supervisory behaviors that emphasized safe work practices ranged from 2.5 to 3.2. The protective effect of working in an environment that included both of the supervisory behaviors was greater than the protective effect of always being provided with safety devices. A sensitivity analysis indicated that the risk ratio estimates were unlikely to be inflated by nonresponse bias.ConclusionsThese results suggest that greater provision of safety devices and interventions aimed at management practices that promote worker safety could substantially reduce the risk of needlestick among U.S. paramedics. Am. J. Ind. Med. 53:866–874, 2010. © 2010 Wiley‐Liss, Inc.
17p13.1 microdeletion involving the TP53 gene in a boy presenting with mental retardation but no tumor
Authors: Schluth‐Bolard Caroline , Sanlaville Damien , Labalme Audrey , Till Marianne , Morin Isabelle , Touraine Renaud , Edery Patrick 
Published: April 2010

AbstractWe report on the diagnosis of a 17p13.1 deletion in a 10‐year‐old boy. The patient presented with mild developmental delay, facial dysmorphism, joint hyperlaxity, pes planus, hypermetropia, hearing loss, and achromic patches following the Blaschko's lines on the right part of the thorax. Chromosome R‐banding was normal. Array CGH using a 244 K oligonucleotide array showed a homogeneous de novo 17p13.1 microdeletion of 400 kb involving TP53 and 25 other genes, including genes involved in brain function (EFNB3, FXR2). To our knowledge, six patients presenting with a constitutional 17p13.1 microdeletion involving the TP53 gene have been reported. We discuss the phenotype of this microdeletion and the genetic counseling issues, especially regarding the risk of cancer associated with the deletion of the TP53 gene. © 2010 Wiley‐Liss, Inc.