bluuebunny/crossref_metadata_2025_split huggingface dataset:
Juvenile habitat partitioning and relative productivity in allochronically isolated sockeye salmon (Oncorhynchus nerka)
Authors: Miller E.K. Fillatre , Bradbury I.R. , Heath D.D. 
Published: November 2011

AbstractAllochronic divergence, like spatial isolation, may contribute to population diversity and adaptation, however the challenges for tracking habitat utilization in shared environments are far greater. Adult Klukshu River (Yukon, Canada) sockeye salmon, Oncorhynchus nerka, return as genetically distinct “early” and “late” runs. Early and late adult spawning populations (1999 and 2000) and their subsequent fry (sampled at 7 sites in 2000 and at 8 sites in 2001 throughout Klukshu Lake and River) were genotyped at eight microsatellite loci. Bayesian assignment was used to determine the spatial distribution of early versus late fry; although intermixed, the distribution of fry significantly differed in Klukshu Lake and in the Klukshu River in 2001, based on crosstab analyses. Late‐run fry predominated in Klukshu Lake at all sites, while early‐run fry were most common in the north and south of Klukshu Lake and in Klukshu River. Early‐run spawners had significantly higher relative productivity (early life survival) than late‐run fish (2.9 times more fry produced per early‐run adult in 2000, and 9.2 times more in 2001). This study demonstrates spatial habitat partitioning and differences in the contribution of allochronically isolated populations to fry abundance, and highlights annual variability that likely contributes to recruitment variation.
The IAT is sensitive to the perceived accuracy of newly learned associations
Authors: Siegel Eric , Sigall Harold , Huber David E. 
Published: November 2011

AbstractThree experiments tested whether the Implicit Association Test (IAT) is sensitive to the perceived accuracy of newly learned associations. In experiment 1, participants learned to associate positive or negative attributes with two novel groups. Participants in one condition were told that the attributes accurately described the groups; in a second condition, prior to learning, they were made aware that the attributes were randomly assigned to the groups. Participants were given an IAT and an explicit measure testing attitudes towards the two groups. When the participants were told that the attributes were accurate, their IAT performance and explicit measure responses indicated a preference for the more positively described group but when the attributes were known to be arbitrary, preferences were reduced according to both measures. Experiment 2 replicated these results and demonstrated that the associations were learned even in the random condition. Experiment 3 included a condition that placed “not” before each attribute, which demonstrated that people can incorporate a negative modifier into a learned association. Explicit attitudes and the IAT showed reversed preferences in this negation condition. These experiments imply that the IAT is sensitive to the perceived accuracy of learned associations. Copyright © 2011 John Wiley & Sons, Ltd.
Justification of rapid prototyping in the development cycle of thermoplastic‐based lab‐on‐a‐chip
Authors: Preywisch Regina , Ritzi‐Lehnert Marion , Drese Klaus S. , Röser Tina 
Published: November 2011

AbstractDuring the developmental cycle of lab‐on‐a‐chip devices, various microstructuring techniques are required. While in the designing and assay implementation phase direct structuring or so‐called rapid‐prototyping methods such as milling or laser ablation are applied, replication methods like hot embossing or injection moulding are favourable for large quantity manufacturing. This work investigated the applicability of rapid‐prototyping techniques for thermoplastic chip development in general, and the reproducibility of performances in dependency of the structuring technique. A previously published chip for prenatal diagnosis that preconcentrates DNA via electrokinetic trapping and field‐amplified‐sample‐stacking and afterwards separates it in CGE was chosen as a model. The impact of structuring, sealing, and the integration of membranes on the mobility of the EOF, DNA preconcentration, and DNA separation was studied. Structuring methods were found to significantly change the location where preconcentration of DNA occurs. However, effects on the mobility of the EOF and the separation quality of DNA were not observed. Exchange of the membrane has no effect on the chip performance, whereas the sealing method impairs the separation of DNA within the chip. The overall assay performance is not significantly influenced by different structuring methods; thus, the application of rapid‐prototyping methods during a chip development cycle is well justified.
Effects of dietary exposure of mink (Mustela vison) to 2,3,7,8-tetrachlorodibenzo-p-dioxin, 2,3,4,7,8-pentachlorodibenzofuran, and 2,3,7,8-tetrachlorodibenzofuran on reproduction and offspring viability and growth
Authors: Moore Jeremy N. , Zwiernik Matthew J. , Newsted John L. , Fitzgerald Scott D. , Link Jane E. , Bradley Patrick W. , Kay Denise , Budinsky Robert , Giesy John P. , Bursian Steven J. 
Published: November 2011

Abstract
This study assessed the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), 2,3,4,7,8-pentachlorodibenzofuran (PeCDF), and 2,3,7,8-tetrachlorodibenzofuran (TCDF) on the reproductive performance of female mink (Mustela vison) and the viability and growth of their offspring. Nine adult female mink were randomly assigned to one of 13 dietary treatments (one control and four doses each of TCDD, PeCDF, and TCDF [2.1–8.4, 4.0–15 and 5.2–25 ng TCDD toxic equivalents (TEQ)/kg body wt/d]). Diets were fed from two months prior to breeding through weaning of offspring at six weeks of age. At least nine kits per treatment group were maintained on their diets through 27 weeks of age. There were no effects on litter size or viability of offspring. No consistent effects were observed on body mass or relative organ masses of animals at any age. 2,3,7,8-Tetrachlorodibenzo-p-dioxin and PeCDF accumulated in the liver and adipose tissue, but TCDF cleared rapidly. The lack of significant effects on reproduction and offspring viability contrasts with effects reported for mink exposed to environmentally derived PCB mixtures with equivalent TCDD potencies. This suggests that it may be inappropriate to apply toxicity reference values associated with PCB mixtures to animals also exposed to TCDD, PeCDF, or TCDF, and the World Health Organization TCDD toxic equivalency factors for some congeners may not be appropriate for mink. Environ. Toxicol. Chem. 2012;31:360–369. © 2011 SETAC
Phytotoxkit: A critical look at a rapid assessment tool
Authors: Van der Vliet Leana , Velicogna Jessica , Princz Juliska , Scroggins Richard 
Published: November 2011

Abstract
Terrestrial plant toxicity testing contributes critical information to many site risk assessments, but standardized tests can be labor-intensive, use large amounts of soil, and employ long test durations. The Phytotoxkit (MicroBioTests, Environmental Bio-Detection Products) minimizes the time and cost associated with terrestrial plant testing with a unique test setup, a shorter test duration, and less soil. However, the sensitivity of the test remains an open question. In this research, the Phytotoxkit and the standardized Environment Canada terrestrial plant toxicity test (definitive test) are compared using a parallel testing approach. Three different scenarios were examined: a multiconcentration test, in which an inhibiting concentration (ICp) was derived from chemically amended soils; a soil remediation test, in which plant growth in a remediated soil was compared to the original contaminated soil; and a site soil test, in which plant growth in a contaminated soil was compared to a reference soil. The contaminants tested were boric acid, Cr(VI) with cyclodextrin as a remediation agent, and petroleum hydrocarbons. Trifolium pratense (red clover) was used in the first and second scenarios, and six different plant species were used in the third scenario. In the first scenario, the Phytotoxkit results compared well with the definitive test results after 5 and 7 d of exposure. In the second scenario, the Phytotoxkit results agreed with the definitive test when evaluating the effectiveness of remediation. In the third scenario, the Phytotoxkit results were often not in agreement with the results from the definitive test. The reduced sensitivity of the Phytotoxkit in the third scenario may be driven by test unit design, as plant roots are separated from soil by filter paper. Environ. Toxicol. Chem. 2012;31:316–323. © 2011 SETAC
Changes in liquid water alter nutrient bioavailability and gas diffusion in frozen antarctic soils contaminated with petroleum hydrocarbons
Authors: Harvey Alexis Nadine , Snape Ian , Siciliano Steven Douglas 
Published: November 2011

Abstract
Bioremediation has been used to remediate petroleum hydrocarbon (PHC)-contaminated sites in polar regions; however, limited knowledge exists in understanding how frozen conditions influence factors that regulate microbial activity. We hypothesized that increased liquid water (θliquid) would affect nutrient supply rates (NSR) and gas diffusion under frozen conditions. If true, management practices that increase θliquid should also increase bioremediation in polar soils by reducing nutrient and oxygen limitations. Influence of θliquid on NSR was determined using diesel-contaminated soil (0–8,000 mg kg−1) from Casey Station, Antarctica. The θliquid was altered between 0.007 and 0.035 cm3cm−3 by packing soil cores at different bulk densities. The nutrient supply rate of NH 4+ and NO 3−, as well as gas diffusion coefficient, Ds, were measured at two temperatures, 21°C and −5°C, to correct for bulk density effects. Freezing decreased NSR of both NH 4+ and NO 3−, with θliquid linked to nitrate and ammonia NSR in frozen soil. Similarly for Ds, decreases due to freezing were much more pronounced in soils with low θliquid compared to soils with higher θliquid contents. Additional studies are needed to determine the relationship between degradation rates and θliquid under frozen conditions. Environ. Toxicol. Chem. 2012;31:395–401. © 2011 SETAC
Baseline characteristics and statistical implications for the OECD 210 fish early-life stage chronic toxicity test
Authors: Oris James T. , Belanger Scott E. , Bailer A. John 
Published: November 2011

Abstract
The fish toxicity assay most commonly used to establish chronic effects is the Organisation for Economic Co-operation and Development (OECD) 210, fish early-life stage test. However, the authors are not aware of any systematic analysis of the experimental design or statistical characteristics of the test since the test guideline was adopted nearly 20 years ago. Here, the authors report the results of an analysis of data compiled from OECD 210 tests conducted by industry labs. The distribution of responses observed in control treatments was analyzed, with the goal of understanding the implication of this variability on the sensitivity of the OECD 210 test guideline and providing recommendations on revised experimental design requirements of the test. Studies were confined to fathead minnows, rainbow trout, and zebrafish. Dichotomous endpoints (hatching success and posthatch survival) were examined for indications of overdispersion to evaluate whether significant chamber-to-chamber variability was present. Dichotomous and continuous (length, wet wt, dry wt) measurement endpoints were analyzed to determine minimum sample size requirements to detect differences from control responses with specified power. Results of the analysis indicated that sensitivity of the test could be improved by maximizing the number of replicate chambers per treatment concentration, increasing the acceptable level of control hatching success and larval survival compared to current levels, using wet weight measurements rather than dry weight, and focusing test efforts on species that demonstrate less variability in outcome measures. From these analyses, the authors provide evidence of the impact of expected levels of variability on the sensitivity of traditional OECD 210 studies and the implications for defining a target for future animal alternative methods for chronic toxicity testing in fish. Environ. Toxicol. Chem. 2012;31:370–376. © 2011 SETAC
Biodegradation of the ultraviolet filter benzophenone-3 under different redox conditions
Authors: Liu You-Sheng , Ying Guang-Guo , Shareef Ali , Kookana Rai S. 
Published: November 2011

Abstract
Biodegradation of the ultraviolet (UV) filter benzophenone-3 (BP-3) was investigated in the laboratory to understand its behavior and fate under oxic and anoxic (nitrate, sulfate, and Fe [III]-reducing) conditions. Biodegradation experiments were conducted in microcosms with 10% of activated sludge and digested sludge under oxic and anoxic conditions, respectively. Benzophenone-3 was well degraded by microorganisms under each redox condition. Under the redox conditions studied, the biodegradation half-life for BP-3 had the following order: oxic (10.7 d) &gt; nitrate-reducing (8.7 d) &gt; Fe (III)-reducing (5.1 d) &gt; sulfate-reducing (4.3 d) ≥ anoxic unamended (4.2 d). The results suggest that anaerobic biodegradation is a more favorable attenuation mechanism for BP-3. Biodegradation of BP-3 produced two products, 4-cresol and 2,4-dihydroxybenzophenone, under oxic and anoxic conditions. Biotransformation of BP-3 to 2,4-dihydroxybenzophenone by way of demethylation of the methoxy substituent (O-demethylation) occurred in cultures under each redox condition. The further biotransformation of 2,4-dihydroxybenzophenone to 4-cresol was inhibited under oxic, nitrate-reducing, and sulfate-reducing conditions. Environ. Toxicol. Chem. 2012;31:289–295. © 2011 SETAC
Simultaneous EEG and MEG source reconstruction in sparse electromagnetic source imaging
Authors: Ding Lei , Yuan Han 
Published: November 2011

AbstractElectroencephalography (EEG) and magnetoencephalography (MEG) have different sensitivities to differently configured brain activations, making them complimentary in providing independent information for better detection and inverse reconstruction of brain sources. In the present study, we developed an integrative approach, which integrates a novel sparse electromagnetic source imaging method, i.e., variation‐based cortical current density (VB‐SCCD), together with the combined use of EEG and MEG data in reconstructing complex brain activity. To perform simultaneous analysis of multimodal data, we proposed to normalize EEG and MEG signals according to their individual noise levels to create unit‐free measures. Our Monte Carlo simulations demonstrated that this integrative approach is capable of reconstructing complex cortical brain activations (up to 10 simultaneously activated and randomly located sources). Results from experimental data showed that complex brain activations evoked in a face recognition task were successfully reconstructed using the integrative approach, which were consistent with other research findings and validated by independent data from functional magnetic resonance imaging using the same stimulus protocol. Reconstructed cortical brain activations from both simulations and experimental data provided precise source localizations as well as accurate spatial extents of localized sources. In comparison with studies using EEG or MEG alone, the performance of cortical source reconstructions using combined EEG and MEG was significantly improved. We demonstrated that this new sparse ESI methodology with integrated analysis of EEG and MEG data could accurately probe spatiotemporal processes of complex human brain activations. This is promising for noninvasively studying large‐scale brain networks of high clinical and scientific significance. Hum Brain Mapp, 2013. © 2010 Wiley Periodicals, Inc.
The influence of local hydrogeologic forcings on near‐stream event water recharge and retention (Upper San Pedro River, Arizona)
Authors: Simpson Scott C. , Meixner Thomas 
Published: November 2011

AbstractThe rise in stream stage during high flow events (floods) can induce losing stream conditions, even along stream reaches that are gaining during baseflow conditions. The aquifer response to flood events can affect the geochemical composition of both near‐stream groundwater and post‐event streamflow, but the amount and persistence of recharged floodwater may differ as a function of local hydrogeologic forcings. As a result, this study focuses on how vertical flood recharge varies under different hydrogeologic forcings and the significance that recharge processes can have on groundwater and streamflow composition after floods.River and shallow groundwater samples were collected along three reaches of the Upper San Pedro River (Arizona, USA) before, during and after the 2009 and 2010 summer monsoon seasons. Tracer data from these samples indicate that subsurface floodwater propagation and residence times are strongly controlled by the direction and magnitude of the dominant stream–aquifer gradient. A reach that is typically strongly gaining shows minimal floodwater retention shortly after large events, whereas the moderately gaining and losing reaches can retain recharged floodwater from smaller events for longer periods. The moderately gaining reach likely returned flood recharge to the river as flow declined. These results indicate that reach‐scale differences in hydrogeologic forcing can control (i) the amount of local flood recharge during events and (ii) the duration of its subsurface retention and possible return to the stream during low‐flow periods. Our observations also suggest that the presence of floodwater in year‐round baseflow is not due to long‐term storage beneath the streambed along predominantly gaining reaches, so three alternative mechanisms are suggested: (i) repeated flooding that drives lateral redistribution of previously recharged floodwater, (ii) vertical recharge on the floodplain during overbank flow events and (iii) temporal variability in the stream–aquifer gradient due to seasonally varying water demands of riparian vegetation. Copyright © 2011 John Wiley & Sons, Ltd.
A nonlinear function approach for the normalized complementary relationship evaporation model
Authors: Han Songjun , Hu Heping , Tian Fuqiang 
Published: November 2011

AbstractA nonlinear function approach for the normalized complementary relationship evaporation model that is different from the methodology maintaining the symmetric complementary relationship with appropriate definitions of potential and wet‐environment evaporation is proposed and verified. This approach employs the definitions used in the advection‐aridity model, wherein the potential is estimated using the Penman equation. Normalized by Penman potential evaporation, the complementary relationship model is expressed as a function describing the relationship between the evaporation ratio (the ratio of the actual to the Penman potential evaporation) and the proportion of the radiation term in Penman potential evaporation. The new nonlinear function proposed in the current study is approximately equivalent to the advection‐aridity and the modified Granger models under conditions that are neither too wet nor too dry, but is more reasonable under arid and wet conditions. The new nonlinear function model performs well in estimating actual evaporation, as verified by the observed data from four sites under different land covers. Copyright © 2011 John Wiley & Sons, Ltd.
Extreme value modelling of daily areal rainfall over Mediterranean catchments in a changing climate
Authors: Tramblay Yves , Neppel Luc , Carreau Julie , Sanchez‐Gomez Emilia 
Published: November 2011

AbstractHeavy rainfall events during the fall season are causing extended damages in Mediterranean catchments. A peaks‐over‐threshold model is developed for the extreme daily areal rainfall occurrence and magnitude in fall over six catchments in Southern France. The main driver of the heavy rainfall events observed in this region is the humidity flux (FHUM) from the Mediterranean Sea. Reanalysis data are used to compute the daily FHUM during the period 1958–2008, to be included as a covariate in the model parameters. Results indicate that the introduction of FHUM as a covariate can improve the modelling of extreme areal precipitation. The seasonal average of FHUM can improve the modelling of the seasonal occurrences of heavy rainfall events, whereas daily FHUM values can improve the modelling of the events magnitudes. In addition, an ensemble of simulations produced by five different general circulation models are considered to compute FHUM in future climate with the emission scenario A1B and hence to evaluate the effect of climate change on the heavy rainfall distribution in the selected catchments. This ensemble of climate models allows the evaluation of the uncertainties in climate projections. By comparison to the reference period 1960–1990, all models project an amplification of the mean seasonal FHUM from the Mediterranean Sea for the projection period 2070–2099, on average by +22%. This increase in FHUM leads to an increase in the number of heavy rainfall events, from an average of 2.55 events during the fall season in present climate to 3.57 events projected for the period 2070–2099. However, the projected changes have limited effects on the magnitude of extreme events, with only a 5% increase in the median of the 100‐year quantiles. Copyright © 2011 John Wiley & Sons, Ltd.
Human papillomavirus in vaginal intraepithelial neoplasia
Authors: Chao Angel , Chen Tse‐Ching , Hsueh Chuen , Huang Chu‐Chun , Yang Jung‐Erh , Hsueh Swei , Huang Huei‐Jean , Lin Cheng‐Tao , Tang Yun‐Hsin , Liou Jui‐Der , Chang Chee‐Jen , Chou Hung‐Hsueh , Lai Chyong‐Huey 
Published: November 2011

AbstractThere are limited data on the prevalence and distribution of human papillomavirus (HPV) genotypes in vaginal intraepithelial neoplasia (VAIN). We sought to clarify this issue in a series of 450 VAIN cases with a confirmed diagnosis between 1990 and 2006. HPV genotyping was performed using paraffin‐embedded specimens and polymerase chain reaction (PCR)‐based methods. Multiple HPV types were validated by E6 type‐specific PCR and direct sequencing. The HPV genotypes of the vaginal and cervical neoplasms were compared for those with incident VAIN and a history of previous/concomitant cervical neoplasms. Ki‐67 was performed for supporting diagnosis of VAIN. Of these 450 VAIN cases (median age, 59 years; range, 19–93), two with missing paraffin blocks and 54 with poor DNA quality were excluded. HPV was detected in 273/394 (69.3%) VAIN, and multiple infections were found in 17.9% of HPV‐positive samples. The leading types were HPV16 (35.5%), HPV58 (9.9%), HPV52 (9.9%), HPV39 (8.4%), HPV33 (7.3%) and HPV53 (7.0%). Among the 156 cases with a history of previous cervical neoplasia, 29.0% had concordant HPV genotypes, while synchronous VAIN samples (n = 49) were more likely to harbor concordant genotypes (58.7%) with the concomitant cervical neoplasm (p = 0.0003). Whether those HPV types in the incident VAIN lesions had existed in the vaginal epithelium at the time of the previous cervical neoplasia or a new acquisition needs to be clarified in prospective follow‐up studies.