bluuebunny/crossref_metadata_2025_split huggingface dataset:
Real‐world anti‐seizure treatment and adverse events among individuals living with drug‐resistant focal epilepsy in the United States
Authors: Mao Jianbin , Takahashi Koji , Cheng Mu , Xu Churong , Boca Andra , Song Yan , Dandurand Ann 
Published: May 2024

AbstractObjectiveThis study aimed to understand how people living with drug‐resistant focal epilepsy (DRE) navigate through lines of antiseizure medications (ASM) and experience adverse events (AEs) in the real‐world setting in the United States.MethodsA retrospective study was conducted with medical chart data from clinical practices in the United States. Eligible adults had a confirmed diagnosis of DRE and initiated a third‐line ASM therapy between January 2013 and January 2020 (i.e., the index date). Subjects must have medical history data available for ≥1 year prior to (the baseline) and ≥2 years after the index date (the follow‐up). Treatment patterns were captured from first to fourth lines. After the emergence of drug resistance, time to ASM discontinuation, reasons for discontinuation, AE experience and AE management were reported separately during third and fourth lines of treatment and beyond.ResultsThe study included a total of 345 individuals, with an average (standard deviation) age of 23.9 (11.9) years at first diagnosis. All individuals had at least three lines of ASMs with first and second lines during baseline, and third line during follow‐up. The first line for most individuals was monotherapy. As individuals progressed through additional lines of ASM therapy, they were more likely to receive polytherapy. The regimens were more individualized after meeting drug resistance criteria. The top reasons for discontinuing were uncontrolled seizure and/or intolerance/AEs for both third and subsequent lines. More than a third of individuals experienced at least one AE. Among those with at least one AE, many individuals had to manage these AEs with dose adjustment (39.4%), discontinuation of offending ASM (37.9%), de novo pharmacotherapy (25.8%), emergency room visit (13.6%), and hospitalization (12.1%).SignificanceThis study demonstrated that individuals living with DRE experience significant AEs, and many of these AEs lead to treatment disruption and significant healthcare resource utilization.Plain Language SummaryThis study examined how individuals with focal epilepsy are treated across various clinics in United States and reported the adverse events these individuals experienced during treatment, along with the consequence associated with these adverse events. We found that as individuals progressed through additional treatments, they were more and more likely to receive more than one antiseizure medication, and a significant portion of individuals experienced at least one adverse event, often manifested as headache, somnolence, dizziness, and fatigue.
Response of Microbial Communities to Naturally Occurring Radioactive Material–Contaminated Sediments: A Microcosm-Based Study
Authors: MacIntosh Amy , Dafforn Katherine , Chariton Anthony , Koppel Darren , Cresswell Tom , Gissi Francesca 
Published: May 2024

Abstract
There is a growing need to understand the potential ecological impacts of contaminants in offshore oil and gas infrastructure, especially if that infrastructure is to be left in situ as a decommissioning option. Naturally occurring radioactive material (NORM) is one type of contaminant found in solid deposits on internal surfaces of infrastructure that poses potential ecological harm if released into the marine environment. Microbes are important components of marine sediment ecosystems because they provide ecosystem services, yet the impacts of NORM contamination to these communities are not well understood. The present study aimed to investigate the response of benthic microbial communities to NORM-contaminated scale, collected from an offshore oil and gas system, via controlled laboratory microcosm studies. Changes to microbial communities in natural sediment and sediments spiked with NORM at radium-226 activity concentrations ranging from 9.5 to 59.8 Bq/kg (in partial equilibria with progeny) over 7 and 28 days were investigated using high-throughput sequencing of environmental DNA extracted from experimental sediments. There were no significant differences in microbial community composition between control and scale-spiked sediments over 7 and 28 days. However, we observed a greater presence of Firmicutes in the scale-mixed treatment and Chloroflexi in the scale-surface treatments after 28 days. This could suggest selection for species with contaminant tolerance or potential resilience to radiation and metal toxicity. Further research is needed to explore microbial tolerance mechanisms and their potential as indicators of effects of radionuclide-contaminated sediments. The present study demonstrated that microcosm studies can provide valuable insights about the potential impacts of contamination from oil and gas infrastructure to sediment microbial communities. Environ Toxicol Chem 2024;43:1648–1661. © 2024 The Authors. Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
Comparison of chemical contaminant measurements using CLAM, POCIS, and silicone band samplers in estuarine mesocosms
Authors: Wirth Ed , Shaddrix Brian , Pisarski Emily , Pennington Paul , DeLorenzo Marie , Whitall David 
Published: May 2024

Abstract
Discrete water samples represent a snapshot of conditions at a particular moment in time and may not represent a true chemical exposure caused by changes in chemical input, tide, flow, and precipitation. Sampling technologies have been engineered to better estimate time-weighted concentrations. In this study, we consider the utility of three integrative sampling platforms: polar organic chemical integrative sampler (POCIS), silicone bands (SBs), and continuous, low-level aquatic monitoring (CLAM). This experiment used simulated southeastern salt marsh mesocosm systems to evaluate the response of passive (POCIS, SBs) and active sampling (CLAM) devices along with discrete sampling methodologies. Three systems were assigned to each passive sampler technology. Initially, all tanks were dosed at nominal (low) bifenthrin, pyrene, and triclosan concentrations of 0.02, 2.2, and 100 µg/L, respectively. After 28 days, the same treatment systems were dosed a second time (high) with bifenthrin, pyrene, and triclosan at 0.08, 8.8, and 200 µg/L, respectively. For passive samplers, estimated water concentrations were calculated using published or laboratory-derived sampling rate constants. Chemical residues measured from SBs resulted in high/low ratios of approximately 2x, approximately 3x, and 1x for bifenthrin, pyrene, and triclosan. A similar pattern was calculated using data from POCIS samples (~4x, ~3x, ~1x). Results from this study will help users of CLAM, POCIS, and SB data to better evaluate water concentrations from sampling events that are integrated across time. Integr Environ Assess Manag 2024;20:1384–1395. © 2024 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).
Key Points
Integrative passive samplers can be an important tool in coastal chemical pollutant monitoring programs, but salt content in marine and estuarine systems can pose a challenge when estimating time-weighted average concentrations. Remote sampling systems that track the total volume extracted, such as the continuous low-level aquatic monitoring (CLAM) unit, may be appropriate for monitoring in remote areas and generating integrated chemical concentrations. Passive sampler devices require sampling rate constants (Rs and KPW) that are sensitive to environmental factors such as salinity. There is a general lack of reported constants measured under saline conditions.
Diffusion tensor imaging from past to present: A bibliometric analysis with trending topics and global productivity during 1980–2022
Authors: Özlük Enes , Özlük Gülşah Öztürk 
Published: May 2024

AbstractDiffusion tensor imaging (DTI) provides increasing opportunities for clinicians. As bibliometric studies define trends of novel modality applications, this study on DTI analyzed global productivity, most prolific institutions, and research areas. Articles obtained from the Web of Science (WoS) database were analyzed with the VOSviewer program, biblioshiny library in the R studio software bibliometrix package, exponential smoothing estimator, and with the SPSS (Version: 22.0) statistical software. In descending order, the United States, China, and Germany were the top 3 countries publishing DTI research. In descending order, the top 3 most productive institutions were California, Harvard and Johns Hopkins University. Trend topics include traumatic brain injury, tract‐based spatial statistics, fractional anisotropy, prognosis, machine learning, autism, white matter integrity and microstructure and Parkinson's disease. According to the estimations, 345 DTI articles will be published in 2025 (95% CI: 254–437). This study indicates the ever‐growing employment of DTI in clinical and basic sciences.
Differences in ankle and knee muscle architecture and plantar pressure distribution among women with knee osteoarthritis
Authors: Cigercioglu Nazli Busra , Bazancir‐Apaydin Zilan , Apaydin Hakan , Baltaci Gul , Guney‐Deniz Hande 
Published: May 2024

AbstractBackgroundThe aim of this study was to compare the plantar pressure distribution and knee and ankle muscle architecture in women with and without knee osteoarthritis (OA).MethodsFifty women with knee OA (mean age = 52.11 ± 4.96 years, mean Body mass index (BMI) = 30.94 ± 4.23 kg/m2) and 50 healthy women as a control group (mean age = 50.93 ± 3.78 years, mean BMI = 29.06 ± 4.82 kg/m2) were included in the study. Ultrasonography was used to evaluate knee and ankle muscles architecture and femoral cartilage thickness. The plantar pressure distribution was evaluated using the Digital Biometry Scanning System and Milleri software (DIASU, Italy). Static foot posture was evaluated using the Foot Posture Index (FPI), and pain severity was assessed using the Visual Analog Scale.ResultsThe OA group exhibited lower muscle thickness in Rectus Femoris (RF) (p = 0.003), Vastus Medialis (VM) (p = 0.004), Vastus Lateralis (p = 0.023), and Peroneus Longus (p = 0.002), as well as lower Medial Gastrocnemius pennation angle (p = 0.049) and higher Fat thickness (FT) in RF (p = 0.033) and VM (p = 0.037) compared to the control group. The OA group showed thinner femoral cartilage thickness (p = 0.001) and higher pain severity (p = 0.001) than the control groups. FPI scores were higher (p = 0.001) in OA group compared to the control group. The plantar pressure distribution results indicated an increase in total surface (p = 0.027), total load (p = 0.002), medial load (p = 0.005), and lateral load (p = 0.002) on dominant side in OA group compared to the control group.ConclusionsKnee and ankle muscle architecture, knee extensor muscle FT, and plantar pressure distribution in the dominant foot differed in individuals with knee OA compared to the control group.
Evaluation of an E6/E7 PCR‐capillary electrophoresis fragment analysis in the genotyping of human papillomavirus in archival FFPE samples of oropharyngeal cancer
Authors: Yin Jian , Li Yufei , Song Cheng , Liu Yin , Zhao Jingjing , Zhao Dongmei , Zhang Shaokai , Zhang Xun , Zhao Fanghui , Qiao Youlin 
Published: May 2024

AbstractAccumulating evidence has demonstrated that high‐risk human papillomaviruses (HR‐HPVs) are involved in the etiology of a subset of oropharyngeal squamous cell carcinoma (OPSCC). In this regard, the International Agency for Research on Cancer (IARC) has recommended direct molecular HPV testing. So far, there is no agreement on the most appropriate method for HPV detection on OPSCC formalin‐fixed paraffin‐embedded (FFPE) materials. In this study, we aimed to evaluate the performance of the high‐sensitive SureX HPV assay in OPSCC FFPE tissues compared with LiPA‐25 and p16ink4a immunostaining. A retrospective series of FFPE primary OPSCC cases were diagnosed between 2008 and 2019 and provided by the Henan Cancer Hospital, China. The level of agreement of two assays was determined using Cohen's Kappa (κ) statistics. A total of 230 FFPE OPSCC samples from tumor resections (n = 160) and diagnostic biopsies (n = 70) were detected. Sixty‐six (28.7%) and 70 (30.4%) samples were identified as HPV‐DNA‐positive by LiPA‐25 and SureX, respectively, of which HPV16 was largely the most common type (95.5% vs 94.3%). We found a perfect concordance between LiPA‐25 and SureX for HPV‐DNA status (κ = 0.906, 95% CI: 0.875–0.937) and for HPV16 (κ = 0.925, 95% CI: 0.897–0.953). In addition, SureX and p16ink4a immunostaining had a perfect concordance (κ = 0.917, 95% CI: 0.888‐0.946). Moreover, the HPV‐driven fraction, based on double positivity for HPV‐DNA and p16ink4a, was similar between SureX (63 of 230, 27.4%) and LiPA‐25 (60 of 230, 26.1%). Similar results were found in samples from resections and biopsies. SureX and LiPA‐25 are comparable. SureX could be used for routine HPV‐DNA detection and genotyping on archival OPSCC FFPE tissues.
Effect of transglutaminase crosslinking combined with lactic fermentation on the potential allergenicity and conformational structure of soy protein
Authors: Xing Guangliang , Yang Siran , Huang Lu , Liu Sitong , Wan Xinyi 
Published: May 2024

AbstractBACKGROUNDFood allergies are a growing concern worldwide, with soy proteins being important allergens that are widely used in various food products. This study investigated the potential of transglutaminase (TGase) and lactic acid bacteria (LAB) treatments to modify the allergenicity and structural properties of soy protein isolate (SPI), aiming to develop safer soy‐based food products.RESULTSTreatment with TGase, LAB or their combination significantly reduced the antibody reactivity of β‐conglycinin and the immunoglobulin E (IgE) binding capacity of soy protein, indicating a decrease in allergenicity. TGase treatment led to the formation of high‐molecular‐weight aggregates, suggesting protein crosslinking, while LAB treatment resulted in partial protein hydrolysis. These structural changes were confirmed by Fourier transform infrared spectroscopy, which showed a decrease in β‐sheet content and an increase in random coil and β‐turn contents. In addition, changes in intrinsic fluorescence and ultraviolet spectroscopy were also observed. The alterations in protein interaction and the reduction in free sulfhydryl groups highlighted the extensive structural modifications induced by these treatments.CONCLUSIONThe synergistic application of TGase and LAB treatments effectively reduced the allergenicity of SPI through significant structural modifications. This approach not only diminished antibody reactivity of β‐conglycinin and IgE binding capacity of soy protein but also altered the protein's primary, secondary and tertiary structures, suggesting a comprehensive alteration of SPI's allergenic potential. These findings provide a promising strategy for mitigating food allergy concerns and lay the foundation for future research on food‐processing techniques aimed at allergen reduction. © 2024 Society of Chemical Industry.
Surgical factors play a critical role in predicting functional outcomes using machine learning in robotic‐assisted total knee arthroplasty
Authors: Lundgren Lisa Spahn , Willems Nathalie , Marchand Robert C. , Batailler Cécile , Lustig Sébastien 
Published: May 2024

AbstractPurposePredictive models help determine predictive factors necessary to improve functional outcomes after total knee arthroplasty (TKA). However, no study has assessed predictive models for functional outcomes after TKA based on the new concepts of personalised surgery and new technologies. This study aimed to develop and evaluate predictive modelling approaches to predict the achievement of minimal clinically important difference (MCID) in patient‐reported outcome measures (PROMs) 1 year after TKA.MethodsFour hundred thirty robotic‐assisted TKAs were analysed in this retrospective study. The mean age was 67.9 ± 7.9 years; the mean body mass index (BMI) was 32.0 ± 6.8 kg/m2. The following PROMs were collected preoperatively and 1‐year postoperatively: knee injury and osteoarthritis outcome score for joint replacement, Western Ontario and McMaster Universities osteoarthritis index (WOMAC) Function, WOMAC Pain. Demographic data, preoperative CT scan, implant size, implant position on the robotic system and characteristics of the joint replacement procedure were selected as predictive variables. Four machine learning algorithms were trained to predict the MCID status at 1‐year post‐TKA for each PROM survey. ‘No MCID’ was chosen as the target. Models were evaluated by class discrimination (F1‐score) and area under the receiver operating characteristic curve (ROC‐AUC).ResultsThe best‐performing model was ridge logistic regression for WOMAC Function (area under the curve [AUC]  = 0.80, F1 = 0.48, sensitivity = 0.79, specificity = 0.62). Variables most strongly contributing to not achieving MCID status were preoperative PROMs, high BMI and femoral resection depth (posterior and distal), supporting functional positioning principles. Conversely, variables contributing to a positive outcome (achieving MCID) were medial/lateral alignment of the tibial component, whether the procedure was an outpatient surgery and whether the patient received managed Medicare insurance.ConclusionThe most predictive variables included preoperative PROMs, BMI and surgical planning. The surgical predictive variables were valgus femoral alignment and femoral rotation, reflecting the benefits of personalised surgery. Including surgical variables in predictive models for functional outcomes after TKA should guide clinical and surgical decision‐making for every patient.Level of EvidenceLevel III.
Design and Construction of Furan‐Based and Thiophene‐Based Salicyladazine Bisbenzoxazine Resins with High Thermal Stability and Tunable Surface Properties
Authors: Kao Yang‐Chin , Mohamed Mohamed Gamal , Chiang Chia‐Husan , Kuo Shiao‐Wei 
Published: May 2024

AbstractTwo disubstituted bisbenzoxazine (Bz) monomers are synthesized using furan (Fa) and thiophene (Th) derivatives: bis((3‐(furan‐2‐ylmethyl)‐7‐ol‐3,4‐dihydro‐2H‐benzo[e][1,3] oxazin‐6‐yl)methylene)hydrazine (BAZ‐Fa‐BZ) and bis((3‐(thiophen‐2‐ylmethyl)‐7‐ol‐3,4‐dihydro‐2H‐benzo[e][1,3] oxazin‐6‐yl)methylene)hydrazine (BAZ‐Th‐BZ). These monomers are synthesized via Mannich condensation of salicylaldazine (1,2‐bis(2,4‐dihydroxybenzylidene)hydrazine (BAZ─4OH)) and paraformaldehyde (CH2O)n, with furfurylamine (FacNH2) and thiophene‐2‐methenamine (Th─NH2), respectively. The chemical structures of BAZ‐Fa‐BZ and BAZ‐Th‐BZ are affirmed using Fourier‐transform infrared spectroscopy (FTIR) and NMR, respectively. A thorough investigation of the thermal polymerization process of BAZ‐Fa‐BZ and BAZ‐Th‐BZ is conducted using differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and in situ FTIR spectra (ranging from 25 to 250 °C). Poly(BAZ‐Fa‐BZ) exhibits superior thermal properties with a thermal decomposition temperature (Td10) of 402 °C and a char yield of 58 wt% after thermal treatment at 250 °C, along with a lower surface free energy of 28.9 mJ m−2 compared to poly(BAZ‐Th‐BZ) (Td10 = 359 °C, char yield = 48 wt%, and surface free energy = 34.1 mJ m−2). Additionally, poly(BAZ‐Th‐BZ/BAZ‐Fa‐BZ) blend with a ratio of 1/3 after thermal curing at 250 °C demonstrates the highest Td10 of 395 °C and a char yield of 60 wt%. Photoluminescence (PL) measurements conducted in the solid state reveal that BAZ‐Th‐BZ, BAZ‐Fa‐BZ, and their blends emit green light when excited at a wavelength of 365 nm.
Tweety homolog 3 promotes colorectal cancer progression through mutual regulation of histone deacetylase 7
Authors: Lu Pengyan , Deng Shumin , Liu Jiaxin , Xiao Qing , Zhou Zhengwei , Li Shuojie , Xin Jiaxuan , Shu Guang , Yi Bo , Yin Gang 
Published: May 2024

AbstractColorectal cancer (CRC) is one of the leading cancers worldwide, with metastasis being a major cause of high mortality rates among patients. In this study, dysregulated gene Tweety homolog 3 (TTYH3) was identified by Gene Expression Omnibus database. Public databases were used to predict potential competing endogenous RNAs (ceRNAs) for TTYH3. Quantitative real‐time polymerase chain reaction, western blot, and immunohistochemistry were utilized to analyze TTYH3 and histone deacetylase 7 (HDAC7) levels. Luciferase assays confirmed miR‐1271‐5p directly targeting the 3′ untranslated regions of TTYH3 and HDAC7. In vitro experiments such as transwell and human umbilical vein endothelial cell tube formation, as well as in vivo mouse models, were conducted to assess the biological functions of TTYH3 and HDAC7. We discovered that upregulation of TTYH3 in CRC promotes cell migration by affecting the Epithelial–mesenchymal transition pathway, which was independent of its ion channel activity. Mechanistically, TTYH3 and HDAC7 functioned as ceRNAs, reciprocally regulating each other's expression. TTYH3 competes for binding miR‐1271‐5p, increasing HDAC7 expression, facilitating CRC metastasis and angiogenesis. This study reveals the critical role of TTYH3 in promoting CRC metastasis through ceRNA crosstalk, offering new insights into potential therapeutic targets for clinical intervention.
Anticipating Tomorrow: Tailoring Parkinson's Symptomatic Therapy Using Predictors of Outcome
Authors: Postuma Ronald B. , Weintraub Daniel , Simuni Tanya , Rodríguez‐Violante Mayela , Leentjens Albert F.G. , Hu Michele T. , Espay Alberto J. , Erro Roberto , Dujardin Kathy , Bohnen Nicolaas I. , Berg Daniela , Mestre Tiago A. , Marras Connie 
Published: May 2024

AbstractBackgroundAlthough research into Parkinson's disease (PD) subtypes and outcome predictions has continued to advance, recommendations for using outcome prediction to guide current treatment decisions remain sparse.ObjectivesTo provide expert opinion‐based recommendations for individually tailored PD symptomatic treatment based on knowledge of risk prediction and subtypes.MethodsUsing a modified Delphi approach, members of the Movement Disorders Society (MDS) Task Force on PD subtypes generated a series of general recommendations around the question: “Using what you know about genetic/biological/clinical subtypes (or any individual‐level predictors of outcome), what advice would you give for selecting symptomatic treatments for an individual patient now, based on what their subtype or individual characteristics predict about their future disease course?” After four iterations and revisions, those recommendations with over 75% endorsement were adopted.ResultsA total of 19 recommendations were endorsed by a group of 13 panelists. The recommendations primarily centered around two themes: (1) incorporating future risk of cognitive impairment into current treatment plans; and (2) identifying future symptom clusters that might be forestalled with a single medication.ConclusionsThese recommendations provide clinicians with a framework for integrating future outcomes into patient‐specific treatment choices. They are not prescriptive guidelines, but adaptable suggestions, which should be tailored to each individual. They are to be considered as a first step of a process that will continue to evolve as additional stakeholders provide new insights and as new information becomes available. As individualized risk prediction advances, the path to better tailored treatment regimens will become clearer.