bluuebunny/crossref_metadata_2025_split huggingface dataset:
DCAF2 regulates the proliferation and differentiation of mouse progenitor spermatogonia by targeting p21 and thymine DNA glycosylase
Authors: Wei Hongwei , Wang Zhijuan , Huang Yating , Gao Longwei , Wang Weiyong , Liu Shuang , Sun Yan‐Li , Liu Huiyu , Weng Yashuang , Fan Heng‐Yu , Zhang Meijia 
Published: June 2024

AbstractDDB1‐Cullin‐4‐associated factor‐2 (DCAF2, also known as DTL or CDT2), a conserved substrate recognition protein of Cullin‐RING E3 ligase 4 (CRL4), recognizes and degrades several substrate proteins during the S phase to maintain cell cycle progression and genome stability. Dcaf2 mainly expressed in germ cells of human and mouse. Our study found that Dcaf2 was expressed in mouse spermatogonia and spermatocyte. The depletion of Dcaf2 in germ cells by crossing Dcaf2fl/fl mice with stimulated by retinoic acid gene 8(Stra8)‐Cre mice caused a reduction in progenitor spermatogonia and differentiating spermatogonia, eventually leading to the failure of meiosis initiation and male infertility. Further studies showed that depletion of Dcaf2 in germ cells caused abnormal accumulation of the substrate proteins, cyclin‐dependent kinase inhibitor 1A (p21) and thymine DNA glycosylase (TDG), decreasing of cell proliferation, increasing of DNA damage and apoptosis. Overexpression of p21 or TDG attenuates proliferation and increases DNA damage and apoptosis in GC‐1 cells, which is exacerbated by co‐overexpression of p21 and TDG. The findings indicate that DCAF2 maintains the proliferation and differentiation of progenitor spermatogonia by targeting the substrate proteins p21 and TDG during the S phase.
From educator to facilitator: Healthcare professionals' experiences of, and views about, delivering a type 1 diabetes structured education programme (DAFNEplus ) informed by behavioural science
Authors: Lawton Julia , Rankin David , Scott Elaine , Lorencatto Fabiana , Gericke Chiara , Heller Simon R. , de Zoysa Nicole ,   the DAFNEplus Study Group
Published: June 2024

AbstractAimsThe DAFNEplus programme incorporates behaviour change techniques into a modified educational intervention and was developed to help address the glycaemic drift observed amongst graduates of standard DAFNE programmes. As the programme's success will be contingent on staff buy‐in, we explored healthcare professionals' experiences of, and views about, delivering DAFNEplus during a clinical trial to help inform decision making about rollout post‐trial.MethodsWe interviewed n = 18 nurses and dieticians who delivered DAFNEplus during the trial. Data were analysed thematically.ResultsWhile many shared initial reservations, all described how their experiences of DAFNEplus programme delivery had had a positive, transformative impact upon their perceptions and working practices. This transformation was enabled by initial training and supervision sessions, the confidence gained from using scripts to support novel programme content delivery, and experiences of delivering the programme and observing DAFNEplus principles being well received by, and having a positive impact on, attendees. Due to these positive experiences, interviewees described a strongly felt ethical mandate to use some DAFNEplus techniques and curriculum content in routine clinical care. While being supportive of a national rollout, they anticipated a variety of attitudinal and logistical (e.g. workload) challenges.ConclusionsThis study provides a vital dimension to the evaluation of the DAFNEplus programme. Interviewees found the intervention to be acceptable and expressed high levels of buy‐in. As well as offering potential endorsement for a national rollout, our findings offer insights which could help inform development and rollout of future behaviour change interventions to support diabetes self‐management.
Cumulative effect of metabolic risk factors on left ventricular geometry in those with versus without early‐onset type 2 diabetes or prediabetes: The Coronary Artery Risk Development in Young Adults (CARDIA) study
Authors: Yoshida Yilin , Zu Yuanhao , Fan Bingbing , Li Shengxu , Yoshida Tadashi , Harville Emily , Zhang Tao , Bae Sejong , Shikany James , Fonseca Vivian A. 
Published: June 2024

AbstractAimTo investigate metabolic risk factors (RFs) that accumulated over 20 years related to left ventricular mass index (LVMI), relative wall thickness (RWT) and LV remodelling patterns in participants with versus without early‐onset type 2 diabetes (T2D) or prediabetes (pre‐D).MethodsA total of 287 early‐onset T2D/pre‐D individuals versus 565 sociodemographic‐matched euglycaemic individuals were selected from the Coronary Artery Risk Development in Young Adults (CARDIA) study, years 0‐25. We used the area under the growth curve (AUC) derived from quadratic random‐effects models of four or more repeated measures of RFs (fasting glucose [FG], insulin, triglycerides [TG], low‐density lipoprotein cholesterol, high‐density lipoprotein cholesterol (HDL‐c), total cholesterol (total‐c), blood pressure and body mass index) to estimate the cumulative burden, and their associations with LV outcomes.ResultsOne standard deviation greater AUC of log (TG) (per 0.48) and HDL‐c (per 13.5 mg/dL) were associated with RWT (β 0.21 and −0.2) in the early‐onset T2D/pre‐D group, but not in the euglycaemia group (β 0.01 and 0.05, P interactions .02 and .03). In both the early‐onset T2D/pre‐D and euglycaemia groups, greater AUCs of log (FG) (per 0.17) and log (insulin) (per 0.43) were associated with higher RWT (β ranges 0.12‐0.24). Greater AUCs of systolic blood pressure (per 10 mmHg) and diastolic blood pressure (per 7.3 mmHg) were associated with higher RWT and LVMI, irrespective of glycaemic status (β ranges 0.17‐0.28). Cumulative TG (odds ratio 3.4, 95% confidence interval: 1.8‐6.3), HDL‐c (0.23, 0.09‐0.59), total‐c (1.9, 1.1‐3.1) and FG (2.2, 1.25‐3.9) were statistically associated with concentric hypertrophy in the T2D/pre‐D group only.ConclusionsSustained hyperglycaemia and hyperinsulinaemia are associated with RWT, and those individuals with early T2D/pre‐D are potentially at greater risk because of their higher levels of glucose and insulin. Dyslipidaemia was associated with LV structural abnormalities in those individuals with early‐onset T2D/pre‐D.
The changing landscape of parenting education programs for divorcing and separating parents: An introduction
Authors: Ferraro Anthony J. , Markham Melinda Stafford 
Published: June 2024

AbstractThis introduction details why there has been an emphasis placed on examining the value and efficacy of divorce and coparenting education programming. A set of articles is then described that, as a collection, provide insight into what is known and where we need to move forward as a field in the best interests of divorcing and separating parents and their children. We highlight seven original articles that provide foundations on this topic and synthesize consistent themes across these articles herein.
Pacific salmon in the Canadian Arctic highlight a range‐expansion pathway for sub‐Arctic fishes
Authors: Dunmall Karen M. , Langan Joseph A. , Cunningham Curry J. , Reist James D. , Melling Humfrey ,   Aklavik Hunters and Trappers Committee,   Olokhaktomiut Hunters and Trappers Committee,   Paulatuk Hunters and Trappers Committee,   Sachs Harbour Hunters and Trappers Committee,   Tuktoyaktuk Hunters and Trappers Committee,   Kugluktuk Hunters and Trappers Organization
Published: June 2024

AbstractRapid climate change is altering Arctic ecosystems at unprecedented rates. These changes in the physical environment may open new corridors for species range expansions, with substantial implications for subsistence‐dependent communities and sensitive ecosystems. Over the past 20 years, rising incidental harvest of Pacific salmon by subsistence fishers has been monitored across a widening range spanning multiple land claim jurisdictions in Arctic Canada. In this study, we connect Indigenous and scientific knowledges to explore potential oceanographic mechanisms facilitating this ongoing northward expansion of Pacific salmon into the western Canadian Arctic. A regression analysis was used to reveal and characterize a two‐part mechanism related to thermal and sea‐ice conditions in the Chukchi and Beaufort seas that explains nearly all of the variation in the relative abundance of salmon observed within this region. The results indicate that warmer late‐spring temperatures in a Chukchi Sea watch‐zone and persistent, suitable summer thermal conditions in a Beaufort Sea watch‐zone together create a range‐expansion corridor and are associated with higher salmon occurrences in subsistence harvests. Furthermore, there is a body of knowledge to suggest that these conditions, and consequently the presence and abundance of Pacific salmon, will become more persistent in the coming decades. Our collaborative approach positions us to document, explore, and explain mechanisms driving changes in fish biodiversity that have the potential to, or are already affecting, Indigenous rights‐holders in a rapidly warming Arctic.
Antimicrobial efficacy of DJK‐5 peptide in combination with EDTA against biofilms in dentinal tubules: Primary irrigation, recovery and re‐irrigation
Authors: Amhmed Mohamed , Liu He , Häkkinen Lari , Haapasalo Markus , Shen Ya 
Published: June 2024

AbstractAimTo investigate the dynamic recovery of biofilms within dentinal tubules after primary irrigation with different protocols, and to evaluate the efficacy of various re‐irrigation protocols on recovered biofilm, considering factors such as smear layer, nutrient conditions, and primary irrigants.MethodologyA total of 416 mono or multi‐species biofilms samples were prepared from human teeth and incubated for 3 weeks. After inducing a smear layer on half of the samples, all specimens were irrigated with one of the following irrigant sequences: (1) 6% NaOCl +17% EDTA, (2) 6% NaOCl +8.5% EDTA, (3) 6% NaOCl and (8.5% EDTA +10 μg/mL DJK‐5 antimicrobial peptide), or (4) sterile water. Thirty‐two samples were used to assess immediate effect, whilst the rest were re‐incubated to assess biofilms recovery. Nutrient conditions were defined based on whether culture media were changed (nutrient‐rich) or not (nutrient‐poor) during re‐incubation. After 16 weeks, recovered biofilms underwent re‐irrigation using four additional protocols, with or without DJK‐5 peptide, based on primary irrigants. Confocal laser scanning microscopy was employed to evaluate immediate irrigant effects, biofilms recovery intervals (1, 3, 5, 8, 12, and 16 weeks after primary irrigation), and re‐irrigation effects at the 16‐week. Statistical analysis included one‐way anova and two‐way mixed anova tests.ResultsThe DJK‐5 peptide irrigation protocols demonstrated the highest killing rates during primary irrigation and resulted in a longer biofilms recovery time of 16 weeks compared to non‐peptide protocols (p < .001). Both primary irrigation type and smear layer presence significantly influenced biofilms recovery (p < .001). In the absence of smear layer, re‐irrigation efficacy didn't significantly differ from primary irrigation, regardless of primary irrigation type or nutrient conditions. However, with a smear layer present, re‐irrigation led to significantly higher proportion of dead bacteria compared to primary irrigation (p < .05). Inclusion of the DJK‐5 peptide into the re‐irrigation protocol displayed superior killing rate compared to other protocols (p < .001).ConclusionsBiofilms exhibited susceptibility to both peptide and non‐peptide protocols during re‐irrigation, irrespective of nutrient conditions or primary irrigation protocols. The DJK‐5 peptide irrigation protocols consistently displayed superior effectiveness compared to non‐peptide protocols.
Time to SARS‐CoV‐2 clearance in African, Caucasian, and Asian ethnic groups
Authors: Ndjengue Nson Louis Sides , Ndombi Delpo Dede Daniella , Lotola Mougeni Fabrice , Bouassa Natacha , Bennjakhoukh Basma , Luthi Alexandra , Voubou Anselme , Atatama Juliette , Tat Pambou Raymond , Mvogo Guy Dieudonné , Sah Victorien , Atangana Bertin , Mveang Nzoghe Amandine , Maloupazoa Siawaya Anicet Christel , Essone Paulin N. , Mougola Bissiengou Pélagie , Ndeboko Bénédicte , Djoba Siawaya Joel Fleury 
Published: June 2024

AbstractBackgroundCOVID‐19 may become a seasonal disease. SARS‐CoV‐2 active circulation coupled with vaccination efforts has undoubtedly modified the virus dynamic. It is therefore important investigate SARS‐CoV‐2 dynamic in different groups of population following the course of spatiotemporal variance and immunization.MethodsTo investigate SARS‐CoV‐2 clearance in different ethnic groups and the impact of immunization, we recruited 777 SARS‐CoV‐2‐positive patients (570 Africans, 156 Caucasians, and 51 Asians). Participants were followed and regularly tested for 2 months until they had two negative tests.ResultsThe vaccination rate was 64.6%. African individuals were less symptomatic (2%), Caucasians (41%) and Asians (36.6%). On average, viral clearance occurred after 10.5 days. Viral load at diagnosis was inversely correlated with viral clearance (p < 0.0001). The time of SARS‐CoV‐2 clearance was higher in Africans and Caucasians than in Asians (Dunn's test p < 0.0001 and p < 0.05, respectively). On average, viral clearance occurred within 9.5 days during the second semester (higher rate of vaccination and SARS‐CoV‐2 exposition), whereas it took 13.6 days during the first semester (lower rate of vaccination and SARS‐CoV‐2 exposition) (Mann–Whitney t‐test p < 0.0001).ConclusionIn conclusion, ethnicity and spatiotemporal changes including SARS‐CoV‐2 exposition and immunization affect SARS‐CoV‐2 clearance.
Like Drifting Sand Dunes: Noisy Lessons in a Porous Field
Authors: Vella Raphael 
Published: June 2024

AbstractThis paper argues that the teaching of art in Higher Educational Institutions is inherently paradoxical. Informed by the transgressive and interdisciplinary qualities of contemporary artistic practices, education nevertheless is often made to fit into a reductionist, outcome‐oriented and individualistic discourse. Taking a weeklong workshop at the Nida Art Colony in Lithuania as a practical axis for its reflections on the fluid nature of art education, the paper discusses possibilities of extending beyond pedagogical, political, human/nonhuman and other borders and treating ‘noise’ and other ‘interferences’ as opportunities for transgression and dialogue. This workshop with students from the Vilnius Academy of Arts took place in September 2022, at a time characterised by the Russia–Ukraine war. Nida's proximity to Russia's exclave Kaliningrad, its location on the narrow Curonian Spit, and its immediate environment characterised by woods and sand dunes provide this paper with a setting for a discussion about a variety of borders: territorial borders, border pedagogies, perceived borders between human and nonhuman entities, between land and sea, and so on. Borders are described as dominant indicators of power and distinction, while educational standards and instruments of measurement often replicate similar distinctions between the known and the unfamiliar. Yet, borders can also be shifted while new connections and dialogues across real and conceptual borders can be forged in a porous process that is predisposed towards flexible scenarios characterised by the ‘not‐yet’. The surrounding forest and wetlands and huge drifting sand dunes in Nida become analogies for the changing structure of the workshop, silently yet overpoweringly advocating for a mutable pedagogy. Analysed through the work of various contemporary artists, this nonhuman intrusion into a pedagogical and creative experience is both undefined and vulnerable, unlike the preordained structures of attainment targets often associated with contemporary schooling.
Migrant Form and Art's Education
Authors: Baldacchino John 
Published: June 2024

AbstractThe fate of art's education looks like a cynical and rather hopeless process. Those who belong to the field pledged their loyalty to the discipline by dint of how they regard art as being inherently pedagogical – what is here called art's education. Decades of engagement in art's education leaves one travelling over spaces that present themselves as widening chasms between art's education (the pedagogical inherence of art) and art education (art's location within the structures of schooled education). Chasms aside, new borders and unnecessary walls keep being erected just as new territories and jurisdictions are continuously declared. Those of us who have always regarded themselves as migrants within the field would be forgiven to pine with nostalgia for when, not so long ago, art and education moved and roamed with pride and autonomy. Maybe it is this nostalgia which keeps us hoping, while knowing very well that often we tend to idealise the very ‘past’ in which we take false solace.
Genomic analysis of genotype–environment interaction in age at first calving of Murrah buffaloes
Authors: dos Santos Jessica Cristina Gonçalves , de Araujo Neto Francisco Ribeiro , de Oliveira Seno Leonardo , de Abreu Santos Daniel Jordan , de Oliveira Katryne Jordana , Aspilcueta‐Borquis Rusbel Raul , de Oliveira Henrique Nunes , Tonhati Humberto 
Published: June 2024

AbstractAge at first calving (AFC) is a measure of sexual maturity associated with the start of productive life of dairy animals. Additionally, a lower AFC reduces the generation interval and early culling of females. However, AFC has low heritability, making it a trait highly influenced by environmental factors. In this scenario, one way to improve the reproductive performance of buffalo cows is to select robust animals according to estimated breeding value (EBV) using models that include genotype–environment interaction (GEI) with the application of reaction norm models (RNMs). This can be achieved by understanding the genomic basis related to GEI of AFC. Thus, in this study, we aimed to predict EBV considering GEI via the RNM and identify candidate genes related to this component in dairy buffaloes through genome‐wide association studies (GWAS). We used 1795 AFC records from three Murrah buffalo herds and formed environmental gradients (EGs) from contemporary group solutions obtained from genetic analysis of 270‐day cumulative milk yield. Heritability estimates ranged from 0.15 to 0.39 along the EG. GWAS of the RNM slope parameter identified important genomic regions. The genomic window that explained the highest percentage of genetic variance of the slope (0.67%) was located on BBU1. After functional analysis, five candidate genes were detected, involved in two biological processes. The results suggested the existence of a GEI for AFC in Murrah buffaloes, with reclassification of animals when different environmental conditions were considered. The inclusion of genomic information increased the accuracy of breeding values for the intercept and slope of the reaction norm. GWAS analysis suggested that important genes associated with the AFC reaction norm slope were possibly also involved in biological processes related to lipid metabolism and immunity.
Molecular mechanism of renal lipid accumulation in diabetic kidney disease
Authors: Fang Zhengying , Liu Ruijie , Xie Jingyuan , He John Cijiang 
Published: June 2024

AbstractDiabetic kidney disease (DKD) is a leading cause of end stage renal disease with unmet clinical demands for treatment. Lipids are essential for cell survival; however, renal cells have limited capability to metabolize overloaded lipids. Dyslipidaemia is common in DKD patients and renal ectopic lipid accumulation is associated with disease progression. Unveiling the molecular mechanism involved in renal lipid regulation is crucial for exploring potential therapeutic targets. In this review, we focused on the mechanism underlying cholesterol, oxysterol and fatty acid metabolism disorder in the context of DKD. Specific regulators of lipid accumulation in different kidney compartment and TREM2 macrophages, a lipid‐related macrophages in DKD, were discussed. The role of sodium‐glucose transporter 2 inhibitors in improving renal lipid accumulation was summarized.
Perceptions of a Shared European Culture Increase the Support for the European Union
Authors: Petrovic Valentina , Walo Simon , Rössel Jörg , Katz‐Gerro Tally , Lopez Belbeze Maria Pilar 
Published: June 2024

AbstractThere is disagreement amongst political theorists as to whether a European demos based on a culturally grounded identity as a precondition for a stronger integration of the European Union (EU) exists. We study a part of this question from a constructivist perspective by analysing how individuals in Europe perceive European culture and how this perception is related to support for the EU. We focus on the following questions: (a) whether European respondents perceive a shared European culture, (b) whether they consider this culture to be of higher value than other cultures and (c) whether they are proud of European cultures. We study these questions on the basis of a survey conducted in 2021 in nine European countries, including both EU and non‐EU countries. Our results show that the perception of a shared European culture and its positive evaluation, both inclusive and exclusive, covary positively with support for the EU.
Carcinogenesis and epidemiology of cervical cancer: The hallmark of human papillomavirus‐associated cancer
Authors: Kobayashi Osamu , Kamata Saki , Okuma Yuki , Nakajima Takahiro , Ikeda Yuji , Saito Keisuke , Kawana Kei 
Published: June 2024

AbstractCervical cancer affects women worldwide and is the most common human papillomavirus (HPV)‐associated cancer. Carcinogenesis caused by HPV results in specific cancer behavior because of the underlying viral infection. The mechanism and timing of the transformation from viral infection to cancer cells have been elucidated in detail. Treatments for this cancer are based on its characteristics and are being implemented. Moreover, HPV infection is widespread worldwide and is transmitted through sexual activity. Although the HPV vaccination is the most effective strategy of preventing cervical cancer, it is not feasible to vaccinate the entire human population especially in low‐ and middle‐income countries. In order to consider the next step for HPV vaccination, we need to understand the characteristics of HPV carcinogenesis and cervical cancer. Additionally, treatment aimed at preservation of reproductive function in patients with cervical cancer is often required, as the cervix is a reproductive organ and because the disease is more prevalent in the adolescent and young adult generation. Thus, there are still many challenges in the diagnosis, treatment, and prevention of cervical cancer.