bluuebunny/crossref_metadata_2025_split huggingface dataset:
Einfluss erhöhter Frischbeton‐ und Umgebungstemperaturen auf die Betoneigenschaften
Authors: Beyer Dries , Neumann Julia , Schack Tobias , Breitenbücher Rolf , Haist Michael 
Published: June 2024

AbstractDer Einfluss des Klimawandels äußert sich in einer stetigen Zunahme an sommerlichen Hitzetagen, wodurch die Wahrscheinlichkeit für das Auftreten erhöhter Frischbetontemperaturen (>30 °C) während der Herstellung und des Einbaus von Beton erheblich zunimmt. Derartige klimatische Randbedingungen stellen für den Betonbau eine Herausforderung dar, da ohne energie‐ und CO2‐intensive Gegenmaßnahmen, wie z. B. Kühlung der Ausgangsstoffe, ein ungewollt hoher Anstieg der Temperatur des Betons bei der Verarbeitung und Erhärtung unausweichlich ist. Dies führt zu einer Beeinflussung der Hydratation, was sich für gewöhnlich in einem beschleunigten Abfall der Verarbeitbarkeit und einer Reduktion der mechanischen Eigenschaften und der Dauerhaftigkeit äußert. Zielsetzung des vorliegenden Beitrags ist es, ein besseres Verständnis des Einflusses erhöhter Frischbetontemperaturen auf die zuvor genannten Eigenschaften zu gewinnen. Weiterhin wurde der Einfluss der Umgebungstemperatur bis zum Betonalter von drei Tagen auf die Betoneigenschaften untersucht. Hierbei wurden Temperaturen bis zu 40 °C betrachtet.
Pioneer factors for DNA replication initiation in metazoans
Authors: Wang Yue , Liang Jing 
Published: June 2024

AbstractPrecise DNA replication is fundamental for genetic inheritance. In eukaryotes, replication initiates at multiple origins that are first “licensed” and subsequently “fired” to activate DNA synthesis. Despite the success in identifying origins with specific DNA motifs in Saccharomyces cerevisiae, no consensus sequence or sequences with a predictive value of replication origins have been recognized in metazoan genomes. Rather, epigenetic rules and chromatin structures are believed to play important roles in governing the selection and activation of replication origins. We propose that replication initiation is facilitated by a group of sequence‐specific “replication pioneer factors,” which function to increase chromatin accessibility and foster a chromatin environment that is conducive to the loading of the prereplication complex. Dysregulation of the function of these factors may lead to gene duplication, genomic instability, and ultimately the occurrence of pathological conditions such as cancer.
Network pharmacology study on fermented soybeans for the prevention of Alzheimer's disease in older individuals
Authors: Shi Shuo‐shuo , Hu Ting 
Published: June 2024

AbstractAlzheimer's disease (AD) is a progressive neurodegenerative disorder characterized by the disruption of synaptic communication among millions of neurons. Recent research has highlighted the potential therapeutic effectiveness of natural polyphenolic compounds in addressing AD. Soybeans are abundant in polyphenols, and their polyphenolic composition undergoes significant alteration through fermentation by Eurotium cristatum. Through comprehensive database searches, we identified active components within fermented soybean polyphenols and genes associated with AD. Subsequently, we utilized Venn diagrams to analyze the overlap between AD‐related genes and these components. Furthermore, we visualized the network between intersecting targets and proteins using Cytoscape software. The anti‐AD effects of soybeans were further explored through comprehensive analysis, including protein–protein interaction analysis, pathway enrichment analysis, and molecular docking studies. Our investigation unveiled 6‐hydroxydaidzein as a major component of fermented soybean polyphenols, shedding light on its potential therapeutic significance in combating AD. The intersection between target proteins of fermented soybeans and disease‐related targets in AD comprised 34 genes. Protein–protein interaction analysis highlighted key potential targets, including glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH), glycogen synthase kinase 3 beta (GSK3B), amyloid precursor protein (APP), cyclin‐dependent kinase 5 (CDK5), and beta‐site APP cleaving enzyme 1 (BACE1). Molecular docking results demonstrated a robust binding effect between major components from fermented soybeans and the aforesaid key targets implicated in AD treatment. These findings suggest that fermented soybeans demonstrate a degree of efficacy and present promising prospects in the prevention of AD.
Comparison of clinical and MRI features of brain metastases between ALK+ and ALK‐ NSCLC
Authors: Ren Xiaolu , Zhang Xuting , Lei Xiaoyan , Ma Weiqin , Zhang Ting , Wang Yuxiang , Ren Jiwei 
Published: June 2024

AbstractBackgroundNon‐small‐cell lung cancer (NSCLC) is the primary cause of brain metastases (BM). This study aimed to investigate differences in clinical and magnetic resonance imaging (MRI) features of BM between anaplastic lymphoma kinase (ALK) gene fusion (ALK+) and ALK wild‐type (ALK‐) NSCLC, and to preliminarily assess the efficacy of radiotherapy for treating BM.MethodsA retrospective analysis included 101 epidermal growth factor receptor (EGFR)‐ NSCLC patients with BM: 41 with ALK gene fusion and 60 being ALK‐. The brain MRI and clinical features were compared between different ALK status using the multivariate analysis, and a nomogram was constructed to predict ALK gene fusion. Fifty‐six patients who did not undergo cerebral surgery and had complete pre‐ and post‐ treatment data were further divided based on whether they received radiotherapy. Log‐rank test was used to compare the short‐term effect of treatment between the two groups under different genotypes.ResultsALK+ BM exhibited decreased peritumoral brain edema size, lower peritumoral brain edema index (PBEI), and a more homogeneous contrast enhancement pattern compared to ALK‐ BM. Age (OR = 1.04; 95%CI: 1.02–1.06), time to BM (OR = 1.50; 95% CI: 1.04–2.14), PBEI (OR = 1.26; 95% CI: 0.97–1.62), smoking status (smoking index >400 vs. non‐smoking status: OR = 1.42; 95% CI: 0.99–2.04) and contrast enhancement pattern (OR = 1.89; 95% CI: 1.28–2.78) were associated with ALK gene fusion. A nomogram based on these variables demonstrated acceptable predictive efficiency (AUC = 0.844). In the ALK+ group, patients who received radiotherapy did not show increased disease control rate (DCR) or progression‐free survival (PFS). In contrast, in the ALK‐ group, those who received radiotherapy had improved objective response rate (ORR), DCR, and PFS compared to those who were only treated with systemic therapy.ConclusionsThe clinical and MRI features of BM can indicate the status of ALK in NSCLC. In the ALK‐ group, patients who received radiotherapy showed higher ORR, DCR, and PFS compared to those who did not.
Extracellular vesicles originating from steatotic hepatocytes promote hepatic stellate cell senescence via AKT/mTOR signaling
Authors: Wu Zongmei , Xia Mengmeng , Wang Junyu , Aguilar Magnolia Martinez , Buist‐Homan Manon , Moshage Han 
Published: June 2024

AbstractThe prevalence of metabolic dysfunction‐associated steatotic liver disease (MASLD) is increasing rapidly due to the obesity epidemic. In the inflammatory stages of MASLD (MASH), activation of hepatic stellate cells (HSCs) leads to initiation and progression of liver fibrosis. Extracellular vesicles (EVs) are released from all cell types and play an important role in intercellular communication. However, the role of EVs released from hepatocytes in the context of MASLD is largely unknown. Therefore, the present study aimed to investigate the role of EVs derived from both normal and steatotic (free fatty acid‐treated) hepatocytes on the phenotype of HSCs via the senescence pathway. Primary rat hepatocytes were treated with free fatty acids (FFAs: oleic acid and palmitic acid). EVs were collected by ultracentrifugation. EVs markers and HSCs activation and senescence markers were assessed by Western blot analysis, qPCR and cytochemistry. Reactive oxygen species (ROS) production was assessed by fluorescence assay. RNA profiles of EVs were evaluated by sequencing. We found that EVs from hepatocytes treated with FFAs (FFA‐EVs) inhibit collagen type 1 and α‐smooth muscle actin expression, increase the production of ROS and the expression of senescence markers (IL‐6, IL‐1β, p21 and senescence‐associated β‐galactosidase activity) in early activating HSCs via the AKT–mTOR pathway. Sequencing showed differentially enriched RNA species between the EVs groups. In conclusion, EVs from FFA‐treated hepatocytes inhibit HSC activation by inducing senescence via the AKT–mTOR signaling pathway. Determining the components in EVs from steatotic hepatocytes that induce HSC senescence may lead to the identification of novel targets for intervention in the treatment of MASLD in the future.
The structure and function of multifunctional protein ErbB3 binding protein 1 (Ebp1) and its role in diseases
Authors: Wang Ying , Xing Jianxiao , Liang Yanyang , Liang Huifang , Liang Nannan , Li Junqin , Yin Guohua , Li Xinhua , Zhang Kaiming 
Published: June 2024

AbstractErbB3‐binding protein 1(Ebp1) has two isoforms, p42 Ebp1 and p48 Ebp1, both of which can regulate cell growth and differentiation. But these isoforms often have opposite effects, including contradictory roles in regulation of cell growth in different tissues and cells. P48 Ebp1 belongs to the full‐length sequence, while conformational changes in the crystal structure of p42 Ebp1 reveals a lack of an α helix at the amino terminus. Due to the differences in the structures of these two isoforms, they have different binding partners and protein modifications. Ebp1 can function as both an oncogene and a tumor suppressor factor. However, the underlying mechanisms by which these two isoforms exert opposite functions are still not fully understood. In this review, we summarize the genes and the structures of protein of these two isoforms, protein modifications, binding partners and the association of different isoforms with diseases.
Effect of ultra‐fine ground whole grain highland barley substitution on wheat dough properties and bread qualities
Authors: Liu Lu , Xu Jingwen , Zhang Ge , Gao Nisi , Xu Xuebing , Zhao Renyong 
Published: June 2024

AbstractBackground and ObjectiveThere is a growing interest in incorporating whole grain highland barley (HB) to wheat bread for improving nutrients and potential health benefits. However, the qualities and sensory properties of whole grain‐based bread were reduced compared to refined wheat‐based bread due to the strong water absorption and large particle size of dietary fiber in whole grain. Therefore, ultra‐fine ground whole grain HB partially substituted 5%–40% wheat flour to make bread, and the relevant dough properties and bread qualities were studied.FindingsThe increased addition of ultra‐fine ground whole grain HB from 5% to 40% to wheat increased dough development time, whereas, decreased dough stability time, elasticity, pH, G′ modulus, and G″ modulus. The increased substitution of ultra‐fine ground whole grain HB to wheat further reduced the specific volume, cohesiveness, elasticity, and resilience of bread, whereas, increased the hardness, adhesiveness, and chewiness of bread.ConclusionsThe partial substitution of wheat with ultra‐fine ground whole grain HB from 5% to 40% wheat significantly affected wheat‐HB dough properties and resultant bread qualities mainly due to weakened gluten networking.Significance and NoveltyThe 5%–10% substitution of wheat by ultra‐fine ground whole grain HB showed the optimum dough properties and bread qualities compared to other treatments, which substituted 20%–40% wheat flour.
Spiritual competencies and social justice: Implications for counselor education pedagogy
Authors: Avent Harris Janeé R. , Gonzales‐Wong Christine D. 
Published: June 2024

AbstractCounselor educators are responsible for creating learning environments that promote students’ increased cultural competence and social justice engagement. Although, counselor educators have expanded their focus on diversity, religion and spirituality remain largely unaddressed in the curriculum and social justice‐oriented instruction. This article provides pedagogical recommendations for counselor educators that promote religious and spiritual competence.
LMNA‐related muscular dystrophy presenting as an inflammatory myopathy
Authors: Almansa Alexandra Santana , Chrzanowski Stephen M. , Rajabi Farrah , Day‐Lewis Megan , Lee Pui Y. , Lidov Hart G. W. , Lehman Laura L. , Hayes Leslie H. 
Published: June 2024

AbstractIntroductionThere are overlapping features between inflammatory myopathies and muscular dystrophies, particularly laminopathies. Key features that characterize laminopathies include axial and proximal weakness, contractures, and cardiac abnormalities.Methods/ResultsA 12‐year‐old girl diagnosed with juvenile dermatomyositis as a child presented with cardiac failure and was found to have an LMNA likely pathogenic variant, with a phenotype most consistent with Emery–Dreifuss muscular dystrophy type 2.DiscussionThe spectrum of clinical features of LMNA‐related muscular dystrophies can mimic or present with inflammatory myopathy‐like features. Early identification of LMNA‐related muscular dystrophies is crucial to ensure appropriate cardiac screening and prevent devastating cardiac complications.
Electrochemical NH‐Sulfoximidation with α‐Keto Acids
Authors: Alam Tipu , Gupta Shalini , Patel Bhisma K. 
Published: June 2024

AbstractAn electrochemical N‐acylation of sulfoximine has been achieved via the coupling of α‐keto acids and NH‐sulfoximines. This process involves the sequential cleavage of C−C bond followed by C(sp2)−N bond formation, with the liberation of H2 and CO2 as the by‐products. A library of N‐aroylated sulfoximines is produced via the coupling of aroyl and sulfoximidoyl radicals by anodic oxidation under constant current electrolysis (CCE). The compatibility of the present protocol has been demonstrated by coupling of various bio‐active compounds, such as NH‐sulfoximine derived from (−)‐borneol, L‐menthol, D‐glucose derivative, and some commercial drugs such as flurbiprofen, and ibuprofen. This late‐stage functionalization highlights the importance of this sustainable protocol. Besides this, various control experiments and detection of H2 evolution have been performed to support the proposed mechanism.
NDT‐C11 as a Viable Novel Detergent for Single Particle Cryo‐EM
Authors: Jiko Chimari , Li Jiannan , Moon Youngsun , Tanaka Yoshito , Gopalasingam Chai C. , Shigematsu Hideki , Chae Pil Seok , Kurisu Genji , Gerle Christoph 
Published: June 2024

AbstractSingle particle cryo electron microscopy (cryo‐EM) is now the major method for the determination of integral membrane protein structure. For the success of a given project the type of membrane mimetic used for extraction from the native cell membrane, purification to homogeneity and finally cryo‐grid vitrification is crucial. Although small molecule amphiphiles – detergents – are the most widely used membrane mimetic, specific tailoring of detergent structure for single particle cryo‐EM is rare and the demand for effective detergents not satisfied. Here, we compare the popular detergent lauryl maltose‐neopentyl glycol (LMNG) with the novel detergent neopentyl glycol‐derived triglucoside‐C11 (NDT‐C11) in its behavior as free detergent and when bound to two types of multisubunit membrane protein complexes – cyanobacterial photosystem I (PSI) and mammalian F‐ATP synthase. We conclude that NDT‐C11 has high potential to become a very useful detergent for single particle cryo‐EM of integral membrane proteins.
An ultra‐low‐power low‐noise amplifier using a combination of current reuse and self‐forward body bias techniques for biomedical applications
Authors: Bijari Abolfazl , Khoshbinahmadi Fereshte , Mallaki Mohammad‐Amin 
Published: June 2024

SummaryLow‐power low noise amplifiers (LNAs) are critical block in the radio frequency (RF) front‐end of medical device radiocommunications service (MedRadio) receivers for amplifying weak received signals while introducing minimal noise. Despite the inherently low transconductance of MOS transistors, it is possible to achieve low noise, high gain, and good linearity in low power LNAs. This paper presents a combination of techniques to realize an ultra‐low‐power LNA with a compact size and high performance. The proposed LNA utilizes multiple gm‐boosting and self‐forward body bias techniques, with certain transistors operating in weak inversion. Additionally, the Multi‐Objective Inclined Planes System Optimization algorithm is employed to determine the optimal values of the circuit components, thereby achieving the best compromise among the various performance parameters. The proposed LNA with buffer is designed and simulated using an 180 nm CMOS process over the frequency band of 0.3–1 GHz. Simulated results show that the proposed LNA achieves a gain of 19.6 dB, a noise figure of 5.1 dB, good input matching (S11 < −10 dB), and a third‐order intermodulation intercept point (IIP3) of 3.8 dBm. Additionally, it consumes 320 μW (without buffer) from a 1 V power supply, and the chip area is 235 μm × 372 μm. The simulated results demonstrate that the proposed ultra‐low power LNA is a promising candidate for biomedical applications.
EUS‐FNA diagnosis of pancreatic tophaceous gout: Two rare cases
Authors: Wang Brant G. , Lee Iris H. 
Published: June 2024

AbstractWe report two patients with pancreatic tophaceous gout diagnosed by endoscopic ultrasound‐guided fine needle aspiration (EUS‐FNA) of presumed cystic mass lesions. The first case involved a patient who had a recent episode of acute pancreatitis 6 months prior, with subsequent imaging concerning for a pseudocyst or mass lesion. The second case involved a patient with epigastric pain associated with a pancreatic head cystic mass and an erroneous original diagnosis of a mucinous pancreatic neoplasm on EUS‐FNA. Diff‐Quik stained direct smears on fresh material obtained from EUS‐FNA of the lesions showed chalky debris with needle shaped negatively birefringent crystals consistent with gout. For the first case, the chalky material was not present on the H&E stained paraffin embedded formalin fixed cellblock slides. The importance of inclusion of cytologic specimen preparations to examine monosodium urate crystals is emphasized.
Evaluation of the Effect of Pistacia atlantica Oleoresin on Blood Sugar, Pressure and Lipids in Patients With Type 2 Diabetes: A Single‐Blind, Placebo‐Controlled Trial
Authors: Memariani Zahra , Tatari Mahin , Zahedi Maryam , Hesari Zahra , Davarian Ali , Kolangi Fatemeh 
Published: June 2024

ABSTRACTBackgroundDiabetes mellitus (DM) is a chronic metabolic disorder characterised by high blood sugar (BS) levels due to impaired insulin production or insulin resistance. It is a global health concern with significant implications for morbidity and mortality. Persian medicine has long utilised natural remedies, such as Pistacia atlantica Desf., for various diseases. In this randomised clinical trial, the effects of P. atlantica oleoresin in the improvement of lipid profiles, glucose indices and blood pressure (BP) were assessed in patients with Type 2 DM.Materials and MethodsIn this randomised, single‐blind, placebo‐controlled study, 42 patients with Type 2 DM were randomly allocated to receive either P. atlantica oleoresin or placebo capsule for 3 months. Patients were evaluated prior to and 12 weeks after the beginning of the intervention, in terms of changes in lipid profiles, glucose indices and BP.ResultsAfter 3 months, the mean BP in patients with DM receiving P. atlantica oleoresin was significantly reduced compared with the baseline (p = 0.001). Also, these changes were significantly higher than those of the control group. The mean of total cholesterol (p = 0.89), low‐density lipoprotein (LDL) (p = 0.43) and triglyceride (TG) (p = 0.98) in the intervention group after 3 months was lower than that in the control group, but this difference was not statistically significant.ConclusionAfter 3 months, there was no significant difference between the P. atlantica and control groups in terms of blood sugar and lipid profiles. The mean BP in patients with DM receiving P. atlantica oleoresin was significantly reduced compared with that in the beginning of the study. Also, these changes were significant compared with the control group.
Environmental DNA can inform the trade‐off between proactive and reactive strategies for crayfish conservation
Authors: Greenhalgh Jack A. , Banks Rebecca , Collins Rupert A. , Juta Ursula , Reeves Sharon , Siggery Ben , Sweet Michael J. , Tibbitts James , Saxon Andrew D. , Warwick Kate E. , Wiseman Glenn , Jones Gareth , Genner Martin J. 
Published: June 2024

AbstractThe introduction of the signal crayfish Pacifastacus leniusculus to British rivers has led to ecological degradation and the decline of the native white‐clawed crayfish Austropotamobius pallipes. To manage and mitigate the impact of the signal crayfish, conservation agencies and government bodies employ multiple conservation strategies. These take the form of proactive native crayfish breeding and stocking programs and reactive invasive crayfish control programs. Here, we used eDNA to assess the populations of native and invasive crayfish species across 50 sites in 10 river catchments in Norfolk, United Kingdom (UK). The sites were chosen to enable assessment of the potential of eDNA to inform proactive and reactive crayfish conservation strategies. Three of the catchments sampled were selected to assess the success of recent A. pallipes reintroduction, whereas the remaining seven were selected to better understand the distribution of each species at the landscape scale. Combining results of eDNA‐based methods with net searches within an occupancy model enabled us to confidently determine the presence of P. leniusculus at eight sites, and A. pallipes at three sites, which was more than visual searches alone (five and two study sites, respectively). Neither eDNA nor net searches detected A. pallipes at sites where A. pallipes had been reintroduced. We recommend that practitioners using eDNA‐based surveys for management and conservation of crayfish should consider: (1) designing eDNA surveys with an emphasis on large spatial scales to comprehensively describe the distributions of native and invasive crayfish in a region of interest; (2) work with local conservation organizations and/or government bodies to inform the selection of study sites to generate results that are meaningful to real‐world conservation actions; and (3) use results from eDNA‐based crayfish surveys to target limited conservation resources to appropriate proactive and/or reactive conservation actions.
Effects of dietary β‐carotene on color and flavor quality of ovaries in adult female Chinese mitten crab (Eriocheir sinensis)
Authors: Zhang Renyue , Zhang Long , Jiang Xiaodong , Wu Xugan , Wang Xichang 
Published: June 2024

AbstractThis study aimed to investigate the effects of adding different levels (0, 50, 100, 200 and 300 mg/kg) of dietary β‐carotene on the color and flavor quality of adult female E. sinensis ovaries. Adding moderate amount of β‐carotene to the feed could regulate the distribution of carotenoids, fatty acids, and free amino acids in the ovaries of E. sinensis, as well as increase the content of total carotenoids, total fatty acids, sweet and umami amino acids in the ovaries. The dietary β‐carotene have limited coloring effects and a significant impact on the flavor of the ovaries. During thermal process, the addition of 100 mg/kg β‐carotene could effectively promote formation of aroma precursors and generate more characteristic aroma components (2‐methylbutanal, 3‐methylbutanal, hexanal, benzaldehyde, methylpyrazine). However, excessive β‐carotene supplementation (300 mg/kg) led to adverse effects on physiological metabolism and flavor of E. sinensis ovaries. Considering both feed cost and quality improvement, β‐carotene supplementation at 100 mg/kg can be considered the optimal feed supplementation for adult female E. sinensis within a 70‐day rearing period to improve edible quality of ovaries.