bluuebunny/crossref_metadata_2025_split huggingface dataset:
Physiochemically and Genetically Engineered Bacteria: Instructive Design Principles and Diverse Applications
Authors: Lin Xia , Jiao Rong , Cui Haowen , Yan Xuebing , Zhang Kun 
Published: June 2024

AbstractWith the comprehensive understanding of microorganisms and the rapid advances of physiochemical engineering and bioengineering technologies, scientists are advancing rationally‐engineered bacteria as emerging drugs for treating various diseases in clinical disease management. Engineered bacteria specifically refer to advanced physiochemical or genetic technologies in combination with cutting edge nanotechnology or physical technologies, which have been validated to play significant roles in lysing tumors, regulating immunity, influencing the metabolic pathways, etc. However, there has no specific reviews that concurrently cover physiochemically‐ and genetically‐engineered bacteria and their derivatives yet, let alone their distinctive design principles and various functions and applications. Herein, the applications of physiochemically and genetically‐engineered bacteria, and classify and discuss significant breakthroughs with an emphasis on their specific design principles and engineering methods objective to different specific uses and diseases beyond cancer is described. The combined strategies for developing in vivo biotherapeutic agents based on these physiochemically‐ and genetically‐engineered bacteria or bacterial derivatives, and elucidated how they repress cancer and other diseases is also underlined. Additionally, the challenges faced by clinical translation and the future development directions are discussed. This review is expected to provide an overall impression on physiochemically‐ and genetically‐engineered bacteria and enlighten more researchers.
Searching for soil: Elucidating public interest in soil and soil conservation from 20 years of internet search trends
Authors: Booth Samuel W. 
Published: June 2024

AbstractOver the last two decades, soil science research has undergone rapid expansion. Understanding public interest in soil science is vital for evaluating dissemination efforts and situating it in the broader environmental discourse. Analyzing Google Trends search data from 2004 to 2023, this study investigates spikes in search volume index (SVI) for soil‐related searches and potential influences. Significant spikes in SVI between 2019–2020 and 2021–2022 were observed for a number of soil characteristics and soil conservation searches. Similar spikes were observed for possible influences such as the documentary “Kiss the ground,” and SVI related to climate change and carbon sequestration. Notably, SVI for “sustainable development goals” aligned with similar patterns in SVI for “soil health,” indicating a possible link between soil interest and the United Nations’ sustainability goals. This study underscores the seemingly rising interest in soil science, possibly linked with dissemination events, and broader environmental concerns and policies.
Neurofilaments and progranulin are related to atrophy in frontotemporal lobar degeneration – A transdiagnostic study cross‐validating atrophy and fluid biomarkers
Authors: Hüper Lea , Steinacker Petra , Polyakova Maryna , Mueller Karsten , Godulla Jannis , Herzig Sabine , Danek Adrian , Engel Annerose , Diehl‐Schmid Janine , Classen Joseph , Fassbender Klaus , Fliessbach Klaus , Jahn Holger , Kassubek Jan , Kornhuber Johannes , Landwehrmeyer Bernhard , Lauer Martin , Obrig Hellmuth , Oeckl Patrick , Prudlo Johannes , Saur Dorothee , Anderl‐Straub Sarah , Synofzik Matthis , Wagner Matias , Wiltfang Jens , Winkelmann Juliane , Volk Alexander E. ,   FTLD Consortium Germany, Huppertz Hans‐Jürgen , Otto Markus , Schroeter Matthias L. 
Published: June 2024

AbstractINTRODUCTIONFrontotemporal lobar degeneration (FTLD) encompasses behavioral variant frontotemporal dementia (bvFTD), progressive supranuclear palsy, corticobasal syndrome/degeneration, and primary progressive aphasias (PPAs). We cross‐validated fluid biomarkers and neuroimaging.METHODSSeven fluid biomarkers from cerebrospinal fluid and serum were related to atrophy in 428 participants including these FTLD subtypes, logopenic variant PPA (lvPPA), Alzheimer's disease (AD), and healthy subjects. Atrophy was assessed by structural magnetic resonance imaging and atlas‐based volumetry.RESULTSFTLD subtypes, lvPPA, and AD showed specific profiles for neurofilament light chain, phosphorylated heavy chain, tau, phospho‐tau, amyloid beta1‐42 from serum/cerebrospinal fluid, and brain atrophy. Neurofilaments related to regional atrophy in bvFTD, whereas progranulin was associated with atrophy in semantic variant PPA. Ubiquitin showed no effects.DISCUSSIONResults specify biomarker and atrophy patterns in FTLD and AD supporting differential diagnosis. They identify neurofilaments and progranulin in interaction with structural imaging as promising candidates for monitoring disease progression and therapy.Highlights
Study cross‐validated neuroimaging and fluid biomarkers in dementia.
Five kinds of frontotemporal lobar degeneration and two variants of Alzheimer's disease.
Study identifies disease‐specific fluid biomarker and atrophy profiles.
Fluid biomarkers and atrophy interact in a disease‐specific way.
Neurofilaments and progranulin are proposed as biomarkers for diagnosis and therapy.
Development of poly(vinyl acetate) adhesive with hot water resistance via constructing semi‐IPN/IPN structures with hydroxymethylated lignin
Authors: Li Jianchun , Chen Guoliang , Han Yanming , Zhang Wei , Zhang Shifeng 
Published: June 2024

AbstractPoly(vinyl acetate) (PVAc) adhesive is often used in the wood industry because it can be rapidly cured at room temperature to produce a high bonding strength. However, PVAc is a linear polymer susceptible to thermal creep, so it will fail to bond in high temperature and humidity environments. In this study, the hot water resistance of PVAc adhesive is improved by introducing lignin with a complex structure to construct multiple cross‐linked networks with a high cross‐link. However, due to the large steric hindrance, the reactivity of lignin hydroxyl groups is low. In the process of preparing adhesive, alkali lignin is hydroxymethylated to improve its reactivity, and then 4,4′‐diphenylmethane diisocyanate is used as a cross‐linker to construct interpenetrating and semi‐interpenetrating polymer networks. The hydroxymethylation occurs through the reaction of formaldehyde with lignin under alkaline conditions. Optimal reaction conditions include a reaction ratio of 0.20, a reaction temperature of 80°C, and a reaction time of 3.5 h. On those conditions, the bonding strength of the lignin‐based PVAc adhesive reaches 1.8 MPa after hot‐water treatment. Therefore, the lignin‐based hot water‐resistant PVAc adhesive has shown good application potential in high temperature and humidity environments.
Catalytically active inclusion bodies as a potential tool for biotechnology
Authors: Bello Muhammad Nura , Sabri Suriana , Mohd Yahaya Normi , Mohd Shariff Fairolniza , Mohamad Ali Mohd Shukuri 
Published: June 2024

AbstractThe initial assumption that viewed inclusion bodies as a hindrance to the efficient production of protein is no longer held due to the emergence of catalytically active inclusion bodies (CatIBs). Recent studies revealed their potential to be used in free form or immobilized as biocatalysts. The curiosity to acquire suitable catalysts has remained the measure of concern for researchers and industrialists. Numerous processes and production in various sectors of food industries, petroleum, pharmaceutical, cosmetics, and many others are still searching for a robust catalyst with outstanding features such as recyclability, resistance to pH, as well as temperature. CatIBs are forms of inclusion bodies that possess catalytic activity, which can improve catalysis efficiency, stability, and recyclability. One of the advantages of CatIBs is their potential to be used as catalysts for numerous bioprocesses when generated by an enzyme. These aggregates can efficiently be used as a replacement for traditional enzyme immobilization. This review tends to focus on the possibility of its application in various processes. The novelty of this review is that it considered the production of CatIBs both from artificial and natural perspectives, as well as how to improve it. Inclusion bodies’ immobilization may provide an efficient alternative in the area of biocatalysis, and hence it will improve industrial sectors and substantially provide a means of achieving excellent performance in the near future.
Do global competitiveness factors impact the marine sustainability practices? An empirical evidence from fisheries sector
Authors: Alsaleh Mohd , Yuan Yuan , Longqi Sun 
Published: June 2024

AbstractAccording to the research on fisheries, there are two main trade‐offs between these sustainability pillars. First, a fisheries industry's developing ecological health diminishes economic gains for fishermen. Second, individual fishermen's financial success undercuts fisheries communities' social goals. This paper aims to investigate the dynamic impact of global competitiveness on the sustainability of the fisheries industry in 27 European countries by considering the production of fisheries, enabling environments, ecosystems for innovation, market size, human capital, and economic growth between 1990 and 2022. At higher quantiles, the findings showed a strong positive association between enabling environment and fisheries sustainability by adopting MMQR with fixed effects, the new method of moments quantile regression. Additionally, in EU27 nations, the impact of human capital was positive and substantial from the first to ninth quantiles. Notably, the findings show that the EU14 developed nations have a greater level of development than the EU13 underdeveloped countries, which considerably benefits fisheries sustainability. Market size and economic growth were also found to condense fisheries sustainability in EU14 developed and EU13 developing countries, supporting the growth hypothesis for fisheries‐producing countries. Innovation ecosystem was found to increase fisheries sustainability across all quantiles. Notably, the findings show that the significant beneficial impact of the innovation ecosystem, market size, and economic development on the sustainability of fisheries is more significant in the emerging nations of the EU13 than in the developed countries of the EU14. With effective and environmentally friendly technology, policymakers may boost market competitiveness in the EU14 and EU13 countries and advance sustainable development objectives.
Long non‐coding RNA as potential diagnostic markers for acute myeloid leukemia: A systematic review and meta‐analysis
Authors: Solomon Yenealem , Berhan Ayenew , Almaw Andargachew , Ersino Tamirat , Damtie Shewaneh , Kiros Teklehaimanot , Fentie Alemie , Chanie Ermias Sisay , Dessie Anteneh Mengist , Alemayehu Ermiyas 
Published: June 2024

AbstractBackgroundAcute myeloid leukemia (AML) is aggressive type of hematological malignancy. Its poses challenges in early diagnosis, necessitating the identification of an effective biomarker. This study aims to assess the diagnostic accuracy of long noncoding RNAs (lncRNA) in the diagnosis of AML through a meta‐analysis. The study is registered on the PROSPERO website with the number 493518.MethodA literature search was conducted in the PubMed, Embase, Hinari, and the Scopus databases to identify relevant studies. We pooled sensitivity, specificity, positive likelihood ratio (PLR), negative likelihood ratio (NLR), diagnostic odds ratio (DOR), and area under the summary receiver operating characteristics (ROC) using Stata 14.1 software. Heterogeneity between studies was determined through the I2 statistic and Cochran‐Q test. A random effect model was chosen due to significant heterogeneity among included studies. Meta‐regression and subgroup analysis were performed to assess the potential source of heterogeneity. Furthermore, potential publication bias was estimated using Deek's funnel plot asymmetry test.ResultsA total of 14 articles covering 19 studies were included in this meta‐analysis comprising 1588 AML patients and 529 healthy participants. The overall pooled sensitivity, specificity, PLR, NLR, DOR, and the area under the summary ROC curve were 0.85 (95% CI = 0.78–0.91), 0.82 (95% CI = 0.72–0.89), 4.7 (95% CI = 2.9–7.4), 0.18 (95% CI = 0.12–0.28), 26 (95% CI = 12–53), and 0.90 (95% CI = 0.87–0.93), respectively. Moreover, lncRNAs from non‐bone marrow mononuclear cells (BMMC) had superior diagnostic value with pooled sensitivity, specificity, and AUC were 0.93, 0.82, and 0.95, respectively.ConclusionThis meta‐analysis demonstrated that circulating lncRNAs can serve as potential diagnostic markers for AML. High accuracy of diagnosis was observed in non‐BMMC lncRNAs, given cutoff value, and the GADPH internal reference gene used. However, further studies with large sample size are required to confirm our results.
miR‐34a promotes the immunosuppressive function of multiple myeloma‐associated macrophages by dampening the TLR‐9 signaling
Authors: Zhang Rui , Zhang Disi , Luo Yilan , Sun Yunyan , Duan Ci , Yang Jiapeng , Wei Jia , Li Xianshi , Lu Yanqi , Lai Xun 
Published: June 2024

AbstractBackgroundPromising outcomes have been observed in multiple myeloma (MM) with the use of immunotherapies, specifically chimeric antigen receptor T (CAR‐T) cell therapy. However, a portion of MM patients do not respond to CAR‐T therapy, and the reasons for this lack of response remain unclear. The objective of this study was to investigate the impact of miR‐34a on the immunosuppressive polarization of macrophages obtained from MM patients.MethodsThe levels of miR‐34a and TLR9 (Toll‐like receptor 9) were examined in macrophages obtained from both healthy individuals and patients with MM. ELISA was employed to investigate the cytokine profiles of the macrophage samples. Co‐culture experiments were conducted to evaluate the immunomodulatory impact of MM‐associated macrophages on CAR‐T cells.ResultsThere was an observed suppressed activation of macrophages and CD4+ T lymphocytes in the blood samples of MM patients. Overexpression of miR‐34a in MM‐associated macrophages dampened the TLR9 expression and impaired the inflammatory polarization. In both the co‐culture system and an animal model, MM‐associated macrophages suppressed the activity and tumoricidal effect of CAR‐T cells in a miR‐34a‐dependent manner.ConclusionThe findings imply that targeting the macrophage miR‐34a/TLR9 axis could potentially alleviate the immunosuppression associated with CAR‐T therapy in MM patients.
Cervical cancer‐specific long non‐coding RNA landscape reveals the favorable prognosis predictive performance of an ion‐channel‐related signature model
Authors: Wang Bochang , Wang Wei , Zhou Wenhao , Zhao Yujie , Liu Wenxin 
Published: June 2024

AbstractBackgroundIon channels play an important role in tumorigenesis and progression of cervical cancer. Multiple long non‐coding RNA genes are widely involved in ion channel‐related signaling regulation. However, the association and potential clinical application of lncRNAs in the prognosis of cervical cancer are still poorly explored.MethodsThirteen patients with cervical cancer were enrolled in current study. Whole transcriptome (involving both mRNAs and lncRNAs) sequencing was performed on fresh tumor and adjacent normal tissues that were surgically resected from patients. A comprehensive cervical cancer‐specific lncRNA landscape was obtained by our custom pipeline. Then, a prognostic scoring model of ion‐channel‐related lncRNAs was established by regression algorithms. The performance of the predictive model as well as its association with the clinical characteristics and tumor microenvironment (TME) status were further evaluated.ResultsTo comprehensively identify cervical cancer‐specific lncRNAs, we sequenced 26 samples of cervical cancer patients and integrated the transcriptomic results. We built a custom analysis pipeline to improve the accuracy of lncRNA identification and functional annotation and obtained 18,482 novel lncRNAs in cervical cancer. Then, 159 ion channel‐ and tumorigenesis‐related (ICTR‐) lncRNAs were identified. Based on nine ICTR‐lncRNAs, we also established a prognostic scoring model and validated its accuracy and robustness in assessing the prognosis of patients with cervical cancer. Besides, the TME was characterized, and we found that B cells, activated CD8+ T, and tertiary lymphoid structures were significantly associated with ICTR‐lncRNAs signature scores.ConclusionWe provided a thorough landscape of cervical cancer‐specific lncRNAs. Through integrative analyses, we identified ion‐channel‐related lncRNAs and established a predictive model for assessing the prognosis of patients with cervical cancer. Meanwhile, we characterized its association with TME status. This study improved our knowledge of the prominent roles of lncRNAs in regulating ion channel in cervical cancer.
Ru(II)‐Catalyzed C−H Alkylation of N‐Benzyltriflamide with Maleimides: Synthesis of o‐Succinimide Substituted Benzaldehydes
Authors: Dethe Dattatraya H. , Kumar Vimlesh , Datta Arnadeep 
Published: June 2024

AbstractA ruthenium‐catalyzed N‐benzyltriflamide assisted C−H alkylation with maleimide followed by hydrolysis of in situ generated imine has been developed for the first time. This synthetic method results in the efficient synthesis of o‐succinimide derivatives of benzaldehydes. This reaction involves less expensive and mild reaction conditions and shows excellent site selectivity and good functional group compatibility.
Temporal trends of cancer incidence rates for the most frequent cancer sites in Cyprus (2004–2017)
Authors: Spartiati Anastasia , Demetriou Anna , Scoutellas V. , Christophi Costas A. , Makris Konstantinos C. 
Published: June 2024

AbstractBackgroundCancer is one of the leading causes of morbidity and mortality, worldwide. Little information is available for the temporal trends of cancer in the Mediterranean region, including Cyprus.AimsWe aimed to analyze cancer incidence trends overall and by sex for the period 2004–2017 regarding the five most common cancer sites for the population of Cyprus.Methods and resultsData were obtained from the nationwide cancer registry dataset that included 27 017 total cancer cases in Cyprus (2004–2017). We estimated the crude, sex‐, and age‐specific, as well as age‐standardized (ASR) cancer incidence rates and we analyzed the time trends of ASR using the joinpoint regression program. For the general population (0–85+ years of age), the most common cancer sites in descending order, were breast, prostate, lung, colorectal, and thyroid cancer. During the study period, breast and thyroid cancer ASR presented a significant (p < .05) increasing temporal trend. Lung cancer ASRs seemed to stabilize (no increase or decrease) during the more recent years (2009 onwards) for both sexes; a similar pattern was observed for colorectal cancer in males. The ASRs of prostate cancer in men were in steady decline from 2012 onwards and the same was observed for the female ASRs of colorectal cancer from 2007 onwards. The colorectal cancer ASR temporal patterns overall, during the whole study period appeared unchanged.ConclusionThis temporal analysis would feed into cancer surveillance and control programs that focus on prevention, early detection, and treatment, particularly for cancer sites of higher mortality rates or those with temporally increasing trends.
Tree‐based model for achieving environmental impact neutrality: A case study application in the agri‐food sector
Authors: Varavallo Giuseppe , Rugani Benedetto , Allocco Marco , Barbera Filippo , Calfapietra Carlo 
Published: June 2024

AbstractConsensus does not currently exists on how to offset the residual environmental footprint generated by agricultural and forestry practices. This paper develops an environmental sustainability management framework to support the achievement of environmental impact neutrality in the primary sector of agriculture and forestry. The proposed framework, named TREEIN, “a TREe model for Environmental Impact Neutrality”, can guide companies toward effective use of existing environmental management and certification schemes that account for both the negative impact of the production system's life cycle and the positive impact offered by adequate ecosystem service supply. First, we did an overview of international standard certifications, protocols, and sustainability programs based on life cycle assessment (LCA) and ecosystem services assessment (ESA) methodologies. Subsequently, we analyzed the sustainability programs in the European‐specific economic sectors of wine and extra‐virgin olive oil, focusing on the combined approach of LCA and ESA methodologies. As a result, TREEIN, a tree model framework, was developed and applied to the case study of a typical farming system in Tuscany (Italy) in order to achievie impact neutrality. Quantitative outcomes, qualitative assertions, and the methodological challenges associated with such a pilot application are investigated and discussed. The TREEIN's benefits and drawbacks are explored in light of the findings of this case study, and a roadmap for its further advancement and adoption in the market is presented.
Icephobic materials and strategies: From bio‐inspirations to smart systems
Authors: Li Xinlin , Liu Yan , Zhang Zhichun , Liu Yanju , Leng Jinsong 
Published: June 2024

AbstractUnwanted ice formations may cause severe functional degradations of facilities and also have a negative impact on their lifespans. Avoiding and removing ice accumulation is always a hot topic in the industrial and technological field. Bionic functional surfaces have been greatly studied for several decades and have proved to be excellent candidates for passive anti‐/deicing applications. However, the drawbacks limit their potential industrial uses under harsh conditions, like low temperatures and high humidity. Most researches on bionic surfaces are focused on a certain function of natural creatures and their underlined fundamental theories are revealed by taking the interface as the static. Actually, living organisms, either plants or animals, are often sensitive and responsive to their surroundings, avoiding risks and even self‐repairing upon damage. From this prospect, a novel view of the bionic icephobic materials has been proposed in the present review, which is expected to be studied and designed by taking the biological species as a system. As two representative icephobic materials, the anti‐/deicing theories of superhydrophobic and slippery surfaces are first discussed. Further, the recent progress of smart icephobic strategies is summarized from interfaces to substrates. We aim to provide new bionic insights on designing future icephobic strategies.