bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anomalous swelling of a polystyrene matrix in organic solvents
Authors: Bernardo Gabriel , Vesely Drahosh 
Published: October 2009

AbstractSwelling behavior of a commercial linear polystyrene, containing a small amount (<5% wt) of mineral oil, has been studied in three different classes of organic solvents (alkanes, alcohols and carboxylic acids) using both gravimetry and light microscopy. A comparison has been made with the results presented in earlier publications using a different linear polystyrene, without mineral oil. It is shown that the polystyrene containing mineral oil absorbs much higher amounts of solvent at lower temperatures then at higher temperatures. This anomalous behavior sharply contrasts with the polystyrene without mineral oil, which at lower temperatures absorbs much less solvent. Light microscopy of the diffusion layer reveals that in the polymer with mineral oil precipitation occurs during diffusion at low temperatures, but not at high temperatures. These results clearly show that the presence of small amounts of mineral oil (in quantities below the detection limit of FTIR) can cause significant changes in the sorption behavior of polymers. These results are interpreted by pre‐existing nano‐inhomogeneities, formed by the mineral oil, limited miscibility of oil in the polymer phase and high miscibility of solvent in the oil phase. © 2009 Wiley Periodicals, Inc. J Appl Polym Sci, 2010
Mono‐, Di‐, Tri‐ and Tetranuclear Rare Earth Complexes Obtained Using a Moderately Bulky Aryloxide Ligand
Authors: Deacon Glen B. , Junk Peter C. , Moxey Graeme J. 
Published: October 2009

AbstractRedox transmetallation ligand exchange reactions involving a rare earth metal, 2,4,6‐trimethylphenol (HOmes), and a diarylmercurial afford rare earth aryloxo complexes, which are structurally characterized. Both the lanthanoid contraction and the identity of the reaction solvent are found to influence the outcome of the reactions. Using THF in the reaction affords a dinuclear species [Ln2(Omes)6(thf)4]⋅2THF (Ln=La 1, Nd 2) for the lighter rare earth metals, while a mononuclear species [Ln(Omes)3(thf)3] (Ln=Sm 3, Tb 5, Er 6, Yb 7, Y 8) is obtained for the heavier rare earth elements. Surprisingly, there is no change in metal coordination number between the two structural motifs. A divalent trinuclear linear complex [Eu3(Omes)6(thf)6] 4 is obtained for Eu, and features solely bridging aryloxide ligands. Using DME as the reaction solvent affords [La(Omes)3(dme)2] 9 from the reaction mixture, and [Ln2(Omes)6(dme)2]⋅PhMe (La 10, Nd 11) and [Y(Omes)3(dme)2] 14 following crystallization of the crude product from toluene. The dinuclear species [Eu2(Omes)4(dme)4] 12 contains two unidentate and two chelating DME ligands, and contrasts the linear structure of 4. Treatment of HOmes and HgPh2 with Yb metal in DME affords the mixed valent YbII/III complex [Yb2(Omes)5(dme)2] 13, which is stabilized by an intramolecular π‐Ph–Yb interaction, and is a rare example of a mixed valent rare earth aryloxide. Treatment of Er metal with HOmes at elevated temperature (solvent free) affords the homoleptic [Er4(Omes)12] 15, which consists of a tetranuclear array of Er atoms arranged in a ‘herringbone’ fashion; the structure is stabilized by intramolecular π‐Ph–Er interactions. Reaction of La metal with HOmes under similar conditions yields toluene insoluble “La(Omes)3”, which affords 1 following extraction with THF.
The role of experience in the Gambler's Fallacy
Authors: Barron Greg , Leider Stephen 
Published: October 2009

AbstractRecent papers have demonstrated that the way people acquire information about a decision problem, by experience or by abstract description, can affect their behavior. We examined the role of experience over time in the emergence of the Gambler's Fallacy in binary prediction tasks. Theories of the Gambler's Fallacy and models of binary prediction suggest that recency bias, elicited by experience over time, may play a significant role. An experiment compared a condition where participants sequentially predicted the colored outcomes of a virtual roulette wheel spin with a condition where the wheel's past outcomes were presented all at once. In a third condition outcomes were presented sequentially in an automatic fashion without intervening predictions. Subjects were yoked so that the same history of outcomes was observed in all conditions. The results revealed the Gambler's Fallacy when outcomes were experienced (with or without predictions). However, the Gambler's Fallacy was attenuated when the same outcomes were presented all at once. Observing the Gambler's Fallacy in the third condition suggests that the presentation of information over time is a significant antecedent of the bias. A second experiment demonstrated that, while the bias can emerge with an all‐at‐once presentation that makes recent outcomes salient (Burns & Corpus, 2004), the bias did not emerge when the presentation did not draw attention to recent outcomes. Copyright © 2009 John Wiley & Sons, Ltd.
Erschöpfte Literatur. Über das Neue bei Samuel Beckett
Authors: Schäfer Armin 
Published: October 2009

AbstractExhausted Literature. The Emergence of the New in Samuel Beckett's Novels and Plays. The article investigates literary subjectivity in some texts by Samuel Beckett. The article proceeds by relating the ways of how narration and speech acts constitute literary subjectivity to the problems of subjectivity that scientific investigations deal with. While successful self‐regulation of the organism nourishes the roots of subjectivity, i. e. the habits, subjectivity decomposes in states of exhaustion, when self‐regulation breaks down. As soon as a certain threshold is transgressed, fatigue sets in, alters the personality and eventually leads to exhaustion – a state, which psychiatrists compare to mental illness. Notwithstanding the different explanations given, scientists agreed about the effects of exhaustion. According to their investigations, the decomposition of personality by exhaustion generally does not involve apathy, withdrawal from activity or termination of movements, but rather mere action. Similarly, in Beckett's novels and plays exhaustion is much more than tiredness, as French philosopher Gilles Deleuze observed. For Beckett, exhaustion is rather the model for both literary innovation and a new concept of subjectivity, which he explores on the basis of a detailed knowledge of physiology, psychology, and psychiatry, but using his own literary means. The exhausted subject is beyond any calculus of activity. It will perform an activity even if he or she makes mistakes or loses control, and will thus act in an unpredictable way. This unpredictable action is not an exception in the continuation of the habits, but rather points to the moment when a new subjectivity emerges. Such new subjectivity surfaces in Beckett's novels and plays in forms of literary innovation.
Homogeneity/Heterogeneity Hypotheses for Standardized Mortality Ratios Based on Minimum Power‐divergence Estimators
Authors: Pardo Leandro , Martín Nirian 
Published: October 2009

AbstractThis paper analyzes the power divergence estimators when homogeneity/heterogeneity hypotheses among standardized mortality ratios (SMRs) are taken into account. A Monte Carlo study shows that when the standard mortality rate is not external, that is it is estimated from the sample data, these estimators have a good performance even for small sample sets and in particular the minimum chi‐square estimators have a better behavior compared to the classical maximum likelihood estimators. In order to make decisions under homogeneity/heterogeneity hypotheses of SMRs we propose some test‐statistics which consider the minimum power divergence estimators. Through a numerical example focused on SMRs of melanoma mortality ratios in different regions of the US, a homogeneity/heterogeneity study is illustrated.
Estimating Antihypertensive Effects of Combination Therapy in an Observational Study Using Marginal Structural Models
Authors: Sugihara Masahiro , Kushiro Toshio , Saito Ikuo , Matsushita Yasuyuki , Hiramatsu Katsutoshi 
Published: October 2009

AbstractThe evaluation of the antihypertensive effect of multiple antihypertensive drugs using data from an observational study requires adjustment for time‐dependent confounders. Marginal structural models (MSMs) have been proposed to address this type of confounding through inverse probability weighting. Generally, the probabilities are estimated using logistic regression models that assume linearity between the logistic link and the predictors, but the linearity might be inaccurate. In this article, we proposed MSMs to assess the blood pressure‐lowering effects of combination therapy with olmesartan medoxomil (OLM) plus calcium channel blockers (CCB) (OLM+CCB) in an observational study of OLM, and extended estimation methods of the probabilities for the MSMs using generalized additive models (GAMs). The estimation using GAMs was suggested to improve the balance of the distributions of confounder values between the therapy groups in the pseudo‐population. We obtained estimated changes in systolic blood pressure (SBP) and diastolic blood pressure (DBP) for OLM+CCB combination therapy after 12 wk compared with OLM monotherapy of −4.3 mmHg (95% confidence interval (CI): −7.7 and −0.9 mmHg) and −2.9 mmHg (95% CI: −5.1 and −0.7 mmHg), respectively. The estimated target BP (SBP<140 mmHg and DBP<90 mmHg) achievement rates for OLM+CCB combination therapy and OLM monotherapy were 62.0 and 46.7%, respectively. The results of the MSMs were closer to those in the randomized controlled trial, such as the combination of OLM and amlodipine besylate in controlling high blood pressure study, than those of conventional methods. The proposed MSMs provided useful information to evaluate the effects of combination therapy of antihypertensive drugs in the context of an observational study.
Neighborhood Dependence in Bayesian Spatial Models
Authors: Assunção Renato , Krainski Elias 
Published: October 2009

AbstractThe conditional autoregressive model and the intrinsic autoregressive model are widely used as prior distribution for random spatial effects in Bayesian models. Several authors have pointed out impractical or counterintuitive consequences on the prior covariance matrix or the posterior covariance matrix of the spatial random effects. This article clarifies many of these puzzling results. We show that the neighborhood graph structure, synthesized in eigenvalues and eigenvectors structure of a matrix associated with the adjacency matrix, determines most of the apparently anomalous behavior. We illustrate our conclusions with regular and irregular lattices including lines, grids, and lattices based on real maps.
Electroporation optimization to deliver plasmid DNA into dental follicle cells
Authors: Yao Shaomian , Rana Samir , Liu Dawen , Wise Gary E. 
Published: October 2009

AbstractElectroporation is a simple and versatile approach for DNA transfer but needs to be optimized for specific cells. We conducted square wave electroporation experiments for rat dental follicle cells under various conditions. These experiments indicated that the optimal electroporation electric field strength was 375 V/cm, and that plasmid concentrations greater than 0.18 μg/μL were required to achieve high transfection efficiency. BSA or fetal bovine serum in the pulsing buffer significantly improved cell survival and increased the number of transfected cells. The optimal pulsing duration was in the range of 45–120 ms at 375 V/cm. This electroporation protocol can be used to deliver DNA into dental follicle cells to study the roles of candidate genes in regulating tooth eruption. This is the first report showing the transfection of dental follicle cells using electroporation. The parameters determined in this study are likely to be applied to transfection of other fibroblast cells.
Mechanistic model for prediction of formate dehydrogenase kinetics under industrially relevant conditions
Authors: Schmidt T. , Michalik C. , Zavrel M. , Spieß A. , Marquardt W. , Ansorge‐Schumacher M. B. 
Published: October 2009

AbstractFormate dehydrogenase (FDH) from Candida boidinii is an important biocatalyst for the regeneration of the cofactor NADH in industrial enzyme‐catalyzed reductions. The mathematical model that is currently applied to predict progress curves during (semi‐)batch reactions has been derived from initial rate studies. Here, it is demonstrated that such extrapolation from initial reaction rates to performance during a complete batch leads to considerable prediction errors. This observation can be attributed to an invalid simplification during the development of the literature model. A novel mechanistic model that describes the course and performance of FDH‐catalyzed NADH regeneration under industrially relevant process conditions is introduced and evaluated. Based on progress curve instead of initial reaction rate measurements, it was discriminated from a comprehensive set of mechanistic model candidates. For the prediction of reaction courses on long time horizons (>1 h), decomposition of NADH has to be considered. The model accurately describes the regeneration reaction under all conditions, even at high concentrations of the substrate formate and thus is clearly superior to the existing model. As a result, for the first time, course and performance of NADH regeneration in industrial enzyme‐catalyzed reductions can be accurately predicted and used to optimize the cost efficiency of the respective processes. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010
High throughput single cell bioinformatics
Authors: Roach Kenneth L. , King Kevin R. , Uygun Basak E. , Kohane Isaac S. , Yarmush Martin L. , Toner Mehmet 
Published: October 2009

AbstractAdvances in systems biology and bioinformatics have highlighted that no cell population is truly uniform and that stochastic behavior is an inherent property of many biological systems. As a result, bulk measurements can be misleading even when particular care has been taken to isolate a single cell type, and measurements averaged over multiple cell populations in a tissue can be as misleading as the average height at an elementary school. There is a growing need for experimental techniques that can provide a combination of single cell resolution, large cell populations, and the ability to track cells over multiple time points. In this article, a microwell array cytometry platform was developed to meet this need and investigate the heterogeneity and stochasticity of cell behavior on a single cell basis. The platform consisted of a microfabricated device with high‐density arrays of cell‐sized microwells and custom software for automated image processing and data analysis. As a model experimental system, we used primary hepatocytes labeled with fluorescent probes sensitive to mitochondrial membrane potential and free radical generation. The cells were exposed to oxidative stress and the responses were dynamically monitored for each cell. The resulting data was then analyzed using bioinformatics techniques such as hierarchical and k‐means clustering to visualize the data and identify interesting features. The results showed that clustering of the dynamic data not only enhanced comparisons between the treatment groups but also revealed a number of distinct response patterns within each treatment group. Heatmaps with hierarchical clustering also provided a data‐rich complement to survival curves in a dose response experiment. The microwell array cytometry platform was shown to be powerful, easy to use, and able to provide a detailed picture of the heterogeneity present in cell responses to oxidative stress. We believe that our microwell array cytometry platform will have general utility for a wide range of questions related to cell population heterogeneity, biological stochasticity, and cell behavior under stress conditions. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Baculovirus P35 protein: An overview of its applications across multiple therapeutic and biotechnological arenas
Authors: Sahdev Sudhir , Saini Kulvinder Singh , Hasnain Seyed E. 
Published: October 2009

AbstractBaculovirus immediate early P35 protein is well known for its anti‐apoptotic as well as anti‐oxidant properties. Mechanism of action of P35 involves inhibition of a vast range of initiator to executioner class of caspases. In addition, P35's role in inhibiting oxidant‐induced mitochondrial damage, primarily in the apoptotic pathway, has also been extensively investigated. Elucidation of P35's functions during regulation of programmed cell death (PCD) has led to a renewed focus on exploiting this basic knowledge for clinical and other related applications. This review outlines specific biochemical and genetic pathways where P35 intervenes and regulates rate‐limiting steps in the apoptotic signaling cascade. Research efforts are underway to utilize P35 as an agent in regulating apoptosis and under certain circumstances, also explore the therapeutic potential of its anti‐oxidant features. One of the major outcomes of recent studies include significantly improved effectiveness of cytochrome P450 directed enzyme pro‐drug delivery tools when used in conjunction with P35, which may help in alleviating drug resistance in tumor cells and simultaneously prolonging the cytotoxic effects of anti‐cancer drugs. Moreover, applied research carried out recently in the fields of diabetes, ischemia‐induced neuronal cell death, experimental autoimmune encephalomyelitis (EAE), multiple sclerosis (MS), inflammatory arthritis, cardiovascular and ocular disorders illustrate P35's utilization across diverse therapeutic areas and will certainly make it an attractive biomolecule for the discovery research. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Chaperone Activity of Bicyclic Nojirimycin Analogues for Gaucher Mutations in Comparison with N‐(n‐nonyl)Deoxynojirimycin
Authors: Luan Zhuo , Higaki Katsumi , Aguilar‐Moncayo Matilde , Ninomiya Haruaki , Ohno Kousaku , García‐Moreno M. Isabel , Ortiz Mellet Carmen , García Fernández José M. , Suzuki Yoshiyuki 
Published: October 2009

AbstractGaucher disease (GD), the most prevalent lysosomal storage disorder, is caused by mutations of lysosomal β‐glucosidase (acid β‐Glu, β‐glucocerebrosidase); these mutations result in protein misfolding. Some inhibitors of this enzyme, such as the iminosugar glucomimetic N‐(n‐nonyl)‐1‐deoxynojirimycin (NN‐DNJ), are known to bind to the active site and stabilize the proper folding for the catalytic form, acting as “chemical chaperones” that facilitate transport and maturation of acid β‐Glu. Recently, bicyclic nojirimycin (NJ) analogues with structure of sp2 iminosugars were found to behave as very selective, competitive inhibitors of the lysosomal β‐Glu. We have now evaluated the glycosidase inhibitory profile of a series of six compounds within this family, namely 5‐N,6‐O‐(N′‐octyliminomethylidene‐NJ (NOI‐NJ), the 6‐thio and 6‐amino‐6‐deoxy derivatives (6S‐NOI‐NJ and 6N‐NOI‐NJ) and the corresponding galactonojirimycin (GNJ) counterparts (NOI‐GNJ, 6S‐NOI‐GNJ and 6N‐NOI‐GNJ), against commercial as well as lysosomal glycosidases. The chaperone effects of four selected candidates (NOI‐NJ, 6S‐NOI‐NJ, 6N‐NOI‐NJ, and 6S‐NOI‐GNJ) were further evaluated in GD fibroblasts with various acid β‐Glu mutations. The compounds showed enzyme enhancement on human fibroblasts with N188S, G202R, F213I or N370S mutations. The chaperone effects of the sp2 iminosugar were generally stronger than those observed for NN‐DNJ; this suggests that these compounds are promising candidates for clinical treatment of GD patients with a broad range of β‐Glu mutations, especially for neuronopathic forms of Gaucher disease.
Open Sesame Technique for chronic total occlusion
Authors: Saito Shigeru 
Published: October 2009

AbstractAngioplasty for chronic total occlusion (CTO) of the coronary artery is still technically challenging. New techniques to improve the success rate are still required. The inability to penetrate guidewires into the CTO lesion through the proximal cap is one of the most difficult situations. When any guidewire cannot penetrate into the CTO lesion, and if the lesion has both a hard proximal cap and a side branch ramifying at the proximal end of the lesion, the insertion of stiff guidewires and/or balloon inflation in the side branch may induce a geometrical shift of the hard plaque. This in turn enables the entry of a guidewire into the CTO lesion. This procedure has been termed the “Open Sesame Technique.” © 2009 Wiley‐Liss, Inc.
The Effect of Bromide Pretreatment on the Performance of Supported Au–Pd Catalysts for the Direct Synthesis of Hydrogen Peroxide
Authors: Ntainjua N. Edwin , Piccinini Marco , Pritchard James C. , He Qian , Edwards Jennifer K. , Carley Albert F. , Moulijn Jacob A. , Kiely Christopher J. , Hutchings Graham J. 
Published: October 2009

AbstractThe effect of pretreating Au–Pd catalysts on MgO and C supports with aqueous bromide solution, prior to using them for the direct synthesis of hydrogen peroxide, has been investigated. These two supports were selected since the parent materials exhibit contrasting microstructures and activities. The carbon‐supported catalysts comprise homogeneous Au–Pd alloy nanoparticles, which give high activity, whereas the MgO‐supported catalyst has Au–Pd alloys with a Pd‐rich surface and a Au‐rich core, which result in lower activity. Pretreatment of these catalysts with bromide was found to enhance H2O2 productivity and the degree of enhancement was largely dependent on the nature of the Au–Pd nanoparticles. Whereas bromide pretreatment significantly enhanced H2O2 productivity over the MgO‐based catalysts, the carbon‐based catalyst only showed a subtle promotional effect. Very low loadings of bromide (0.00034–0.044 wt %) were required to yield a significant positive effect. Higher bromide loadings (0.5–8.3 wt %) proved deleterious. The promotional effect has been correlated to selective poisoning of sites responsible for H2O2 hydrogenation and decomposition. In view of the limited effect of bromide pretreatment on the yield of H2O2 coupled with the effective performance of the carbon supported Au‐Pd catalysts in the absence of halides, for practical processes the addition of halides is not considered advisable with this catalyst system.
Structural Diversity of Infinite 3d–4f Heterometallic Cluster Compounds Driven by Various Lanthanide Radii
Authors: Xiang Shengchang , Hu Shengmin , Sheng Tianlu , Chen Jianshan , Wu Xintao 
Published: October 2009

AbstractThe syntheses, structures, and characterization of six Ln3+–Cu2+–glycine (Hgly) coordination polymers are described in this paper. They represent three types of structures. Type I (Ln=La (1), Pr (2), and Sm (3)) is a 1D catenarian polymer comprising [Ln2] nodes bridged by four cis‐Cu(gly)2 linkers. Type II (Ln=Eu (4) and Dy (5)) is a 2D open framework with a 44‐net, composed of novel [Ln6Cu22] cluster nodes linked by trans‐Cu(gly)2 linkers. Furthermore, the inner structures of the [Ln6Cu22] nodes, and the connection mode between the nodes and linkers are slightly different for 4 and 5. Type III (Ln=Er (6)) is a 3D open framework with a novel 36⋅418⋅53⋅6 topology, made up of [Er6Cu24] cluster nodes and trans‐Cu(gly)2 linkers. The rich variety of the resulting structures owes itself mainly to the interselection between the dynamic control of metalloligands and cationic components. A transition from frequency dependence to frequency independence is observed in the field‐induced magnetization lag for 1–3. The frequency dependence at low temperatures may come from the antiferromagnetic CuCu interaction through the [Ln2] nodes, whereas the frequency independence may be due to the disappearance of the antiferromagnetic CuCu interaction at high temperatures.