bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of ascites tumor growth in vivo by sTie‐2 is potentiated by a combinatorial therapy with sFLT‐1
Authors: D'Souza Saritha Sandra , Gururaj Anupama E. , Raj Harsha M. , Rössler Jochen , Salimath Bharathi P. 
Published: November 2010

AbstractBackgroundInhibition of tumor angiogenesis is a promising approach for cancer therapy and the Tie‐2/angiopoietin pathway appears to play an important role. In the present study, we have developed strategies to explore the therapeutic potential of blocking the Tie‐2/angiopoietin pathway by sTie‐2.MethodsEhrlich ascites tumor (EAT) cells were stably transfected to overexpress a truncated form of sTie‐2. Transfectants were characterized for their in vitro growth behavior and transplanted into nude mice. Furthermore, recombinant sTie‐2 produced by the baculovirus expression system was used to sequester angiopoietins in the murine ascites carcinoma model. The effect of sTie‐2 treatment alone or in combination with sFLT‐1 on the weight of the animal, ascites cell number and volume was studied.ResultsEAT cells stably transfected with a truncated form of sTie‐2 showed no change in cell proliferation in vitro and colony forming in soft agar compared to control cells. However, sTie‐2 transfected EAT cells transplanted into nude mice reduced tumor burden and demonstrated a reduction in ascites formation and peritoneal angiogenesis. Recombinant sTie‐2 showed angiogenic activity in the tube formation and wound healing assay in vitro. sTie‐2 treatment alone or in combination with sFLT‐1 in an ascites tumor mouse model resulted in reduced peritoneal angiogenesis, with a concomitant decrease in tumor cell number, volume of ascites and the number of invasive tumor cells, as assayed by CD31 staining.ConclusionsThe findings of the present study demonstrate an important role for the Tie‐2/angiopoietin pathway in the formation of tumor vasculature and suggest that sTie‐2 might yield useful anticancer therapy. Copyright © 2010 John Wiley & Sons, Ltd.
Temperature and effective moisture variability in southern Finland during the Holocene quantified with midge‐based calibration models
Authors: Luoto Tomi P. , Kultti Seija , Nevalainen Liisa , Sarmaja‐Korjonen Kaarina 
Published: November 2010

AbstractEffective moisture and temperature dynamics during the late glacial and Holocene were reconstructed from fossil midge (Diptera: Nematocera) assemblages in Lake Hirvijärvi, southern Finland. Quantitative inferences were performed using midge‐based transfer functions for water depth and mean July air temperature. The aim was to identify major trends in Holocene climate development. The results were generally consistent with patterns found in previous investigations from nearby regions and indicated some distinct differences in palaeoclimate between southern and northern Finland. The results indicate that the region was characterised by a very cold Late Pleistocene, cool and moist but drying early Holocene, warm and moist mid Holocene and cool and moist late Holocene. A progressive trend of increasing lake levels from the early Holocene towards the present was evident. The results of this study provide implications for regional differences in effective moisture between southern and northern Finland during the Holocene. Copyright © 2010 John Wiley & Sons, Ltd.
Early‐middle Holocene land snail shell stable isotope record from Grotta di Latronico 3 (southern Italy)
Authors: Colonese André C. , Zanchetta Giovanni , Dotsika Elisabeth , Drysdale Russell N. , Fallick Anthony E. , Grifoni Cremonesi Renata , Manganelli Giuseppe 
Published: November 2010

AbstractThis paper compares stable isotope (δ18O and δ13C) records of early–middle Holocene land snail shells from the archaeological deposits of Grotta di Latronico 3 (LTR3; southern Italy) with modern shell isotopic data. No substantial interspecific variability was observed in shell δ18O (δ18Os) of modern specimens (Pomatias elegans,Cornu aspersum,Eobania vermiculata,Helix ligataandMarmorana fuscolabiata). In contrast, interspecific shell δ13C (δ13Cs) variability was significant, probably due to different feeding behaviour among species. The δ18Os values of living land snails suggest that species hibernate for a long period during colder months, so that the signal of18O‐depleted winter rainfall in their δ18Os is lost. This suggests that δ18Os and δ13Cs values ofPomatias elegansfrom this archaeological succession provide valuable clues for seasonal (spring–autumn) climatic conditions during the early–middle Holocene. The δ18Os values of fossil specimens are significantly lower than in modern shells and in agreement with other palaeoclimatic records, suggesting a substantial increase of precipitation and/or persistent changes in air mass source trajectories over this region between ca. 8.8 cal ka BP and 6.2–6.7 ka ago. The δ13Cs trend suggests a transition from a slightly13C‐enriched to a13C‐depleted diet between early and middle Holocene compared to present conditions. We postulate that this δ13Cs trend might reflect changes in the C3 vegetation community, potentially combined with other environmental factors such as regional moisture increase and the progressive decrease of atmospheric CO2concentration. Copyright © 2010 John Wiley & Sons, Ltd.
Can 234U–230Th dating be used to date large semi‐arid tufas? Challenges from a study in the Naukluft Mountains, Namibia
Authors: Stone Abigail E.C. , Viles Heather A. , Thomas Louise , Van Calsteren Peter 
Published: November 2010

AbstractThe large, extensive tufa deposits of the semi‐arid Naukluft Mountains, Namibia are potentially important palaeoenvironmental indicators in an area with few proxy records. Tufas are reliable indicators of increased moisture availability, and have been shown to be amenable to 234U–230Th dating, although two challenges are detrital contamination and open‐system behaviour. Densely cemented tufa facies are good candidates for dating, minimising these problems. We report attempts to date five densely‐cemented units, which are only found rarely within the Naukluft deposits. We applied a detailed methodology using multiple subsample analysis, measurement of insoluble residues, application of ‘isochron’ mixing lines, and attempted open‐systems modelling, alongside observations of micromorphology and cathodoluminescence in order to assess the validity of any obtained dates. Surprisingly, densely cemented tufas were found not always to be suitable for dating. Two units contained detrital contamination, which could not be corrected for using a single leachate correction or ‘isochron’ methods. Two units contained ‘excess 230Th’. This could result under a closed‐system if initial (234U/238U) was sufficiently high. Alternatively this may be the result of open‐system behaviour, and loss of uranium, or incorporation of initial unsupported 230Th, which render samples unsuitable for 234U–230Th dating. Micromorphological appearance and cathodoluminescence behaviour are used to explore these possibilities. This study exemplifies the need for careful sample selection, and highlights the importance of analysing multiple subsamples from any tufa sample. The detailed methodology applied proves to be a powerful tool for identifying the range of problems that can be encountered when selecting suitable candidate samples for successful dating. It also shows that semi‐arid tufa sequences may contain very little material suitable for dating. A reliable age of c 80 ka was obtained for a banded unit within a large fluvial barrage, with less reliable dates suggesting tufa deposition during times since >350 ka through to the late Holocene. Copyright © 2010 John Wiley & Sons, Ltd.
Early Devensian (MIS 5d–5b) occupation at Dartford, southeast England
Authors: Wenban‐Smith Francis F. , Bates Martin R. , Schwenninger Jean‐Luc 
Published: November 2010

AbstractA deeply buried horizon containing mint‐condition flint artefacts was discovered in 2006 during archaeological investigations in advance of major roadworks near Dartford, Kent, in southeast England. The context of the artefacts and the freshness of their condition suggest this horizon represents a buried occupation surface. Optically stimulated luminescence dating places this horizon in the period Marine Isotope Stage 5d–5b, early in the British Devensian glaciation. This paper describes details of the artefacts, their context and dates, and outlines how this apparent occupation fits in with the wider pattern of Neanderthal settlement in Britain and northwest Europe in the later Pleistocene. Copyright © 2010 John Wiley & Sons, Ltd.
SERS study of Ag nanoparticles electrodeposited on patterned TiO2 nanotube films
Authors: Huang Yongxia , Sun Lan , Xie Kunpeng , Lai Yuekun , Liu Biju , Ren Bin , Lin Changjian 
Published: November 2010

AbstractIn this work, Ag nanoparticles (NPs) were deposited on patterned TiO2 nanotube films through pulse‐current (PC) electrodeposition, and as a result patterned Ag NPs films were achieved. Scanning electron microscopy (SEM), electron probe microanalysis (EPMA), and X‐ray diffraction (XRD) were used, respectively, to study the morphology, uniformity, and phase structure of the patterned Ag NP films. The size and density of the as‐deposited Ag NPs could be controlled by changing the deposition charge density, and it was found that the patterned Ag NP films produced under a charge density of 2.0 C cm−2 gave intense UV–vis and Raman peaks. Two‐dimensional surface‐enhanced Raman scattering (SERS) mapping of rhodamine 6G (R6G) on the patterned Ag NP films demonstrated a high‐throughput, localized molecular adsorption and micropatterned SERS effect. Copyright © 2010 John Wiley & Sons, Ltd.
Time‐resolved Raman studies on Al2O3: Cr3+: lifetime measurements of the excited‐state transition Ē → 2Ā
Authors: Tobon Yeny A. , Bormann Denis , Canizares Aurélien , Raimboux Nicole , Simon Patrick 
Published: November 2010

AbstractA time‐resolved intensified charge coupled device‐based Raman microspectrometer system dedicated to the study of solid samples is described, offering good optical, temporal and spatial resolution. The advantages of this approach are demonstrated on Al2O3:Cr3+, obtaining for the first time the temporal evolution of the excited state transition Ē → 2Ā. Moreover, the time dependence of the luminescence due to the chromium ion was also determined by the same Raman device. Copyright © 2010 John Wiley & Sons, Ltd.
Temperature‐dependent Raman scattering studies on Na2Mo2O7 disodium dimolybdate
Authors: Saraiva G. D. , Paraguassu W. , Maczka M. , Freire P. T. C. , de Sousa F. F. , Mendes Filho J. 
Published: November 2010

AbstractTemperature–dependent Raman studies of disodium dimolybdate (Na2Mo2O7) crystal are reported. Lattice dynamical calculation was used to predict both wavenumbers and atomic displacements (eigenvectors) for the vibrational modes. These calculations were based on the classical rigid‐ion model. The high‐temperature Raman scattering study of the crystal showed that it remains in the orthorhombic structure in the 8–848 K range and undergoes a structural phase transition between 848 and 854 K. This phase transition is most likely connected with weak tiltings and/or rotations of both MoO4 (tetrahedra) and MoO6 (octahedra) units, which lead to a disorder in the oxygen sublattice. Copyright © 2010 John Wiley & Sons, Ltd.
Clinicopathologic characteristics and prognosis of gastric cancer invading the subserosa
Authors: Jiang Cheng‐Gang , Wang Zhen‐Ning , Sun Zhe , Liu Fu‐Nan , Yu Miao , Xu Hui‐Mian 
Published: November 2010

AbstractBackgroundThe clinicopathologic characteristics and prognosis for gastric cancer invading subserosa (SS) are still not clear.MethodsA retrospective analysis was under taken of 1,201 patients with advanced gastric cancer (pT2, pT3, and pT4a) who had undergone gastrectomy from 1980 to 2001. Clinicopathologic characteristics and prognosis of gastric cancer invading SS were compared with those of cancers invading muscularis propria (MP) and serosa (Se).ResultsPatients with cancers invading SS had larger tumors and more frequent lymphovascular invasion, lymph node and peritoneal metastasis than those with cancers invading MP. Yet, they had smaller tumors and less frequent lymphovascular invasion, lymph node and peritoneal metastasis than those with cancers invading Se. Postoperative survival with cancers invading SS was intermediate between that with cancers invading MP and Se at pN0, pN1, pN2, and pN3 stages. Multivariate analysis indicated lymph node metastasis, type of resection, curability, peritoneal metastasis, and hepatic metastasis were independent prognostic factors for patients with cancers invading SS.ConclusionsClinicopathologic characteristics and prognosis of gastric cancer invading SS are intermediate between those of gastric cancers invading MP and Se. J. Surg. Oncol. 2010;102:737–741. © 2010 Wiley‐Liss, Inc.
Pharmacology of perioperative 5‐Fluorouracil
Authors: Van der Speeten Kurt , Stuart O. Anthony , Mahteme Haile , Sugarbaker Paul H. 
Published: November 2010

AbstractBackgroundThe purpose of this study was to analyze our current pharmacologic data regarding the perioperative use of 5‐fluorouracil in the treatment of peritoneal surface malignancies.MethodsTwenty‐nine patients with peritoneal carcinomatosis from appendiceal malignancy were included in this pharmacological study.ResultsIn the nine patients who received early postoperative intraperitoneal chemotherapy, the area under the curve for intraperitoneal 5‐fluorouracil was 43,000 (±20,300) µg/ml × min and for intravenous 5‐fluorouracil was 157 (±99) µg/ml × min. The area under the curve ratio was 422 (±360). In 20 patients who received intravenous 5‐fluorouracil in the operating room intraperitoneal 5‐fluorouracil levels maintained a higher level as compared to the intravenous drug level over the 90 min of drug sampling. The area under the curve ratio of peritoneal fluid to plasma was 2.3 (±1.3). The area under curve ratio of peritoneal fluid to tumor nodules was 9.9 (±9.8). The area under the curve ratio of plasma to tumor nodules was 5.2 (±4.7).ConclusionsBy modulating the route or timing of administration of 5‐fluorouracil, it becomes a pharmacologic advantageous molecule in patients with peritoneal carcinomatosis of an appendiceal malignancy. 5‐fluorouracil remains the cornerstone of the perioperative management of peritoneal carcinomatosis of gastrointestinal origin. J. Surg. Oncol. 2010;102:730–735. © 2010 Wiley‐Liss, Inc.
Significance of aeg‐1 expression in correlation with vegf, microvessel density and clinicopathological characteristics in triple‐negative breast cancer
Authors: Li Cong , Li Rui , Song Hongtao , Wang Dong , Feng Tian , Yu Xiaoguang , Zhao Yulan , Liu Junjun , Yu Xiaoyu , Wang Yanbo , Geng Jingshu 
Published: November 2010

AbstractPurposeOur study is to examine astrocyte‐elevated gene‐1 (AEG‐1) expression in triple‐negative breast cancer and to determine whether it is associated with vascular endothelial growth factor (VEGF), microvessel density (MVD), clinicopathological parameters and poor survival.MethodsSpecimens from 125 patients with triple‐negative breast cancers were investigated by immunohistochemistry for MVD, AEG‐1 and VEGF expression. Correlations between the expression of AEG‐1, VEGF, MVD, and various clinicopathological factors including survival status were studied.ResultsAEG‐1 and VEGF were highly expressed in 56.8% and 52.8% of triple‐negative breast cancer patients, respectively. The intensity of AEG‐1 was gradually up‐regulated from VEGF−MVD−low, VEGF−high, or MVD−high to VEGF−MVD−high tissues using Western blot analysis. Statistically significant correlation was found among AEG‐1 and VEGF, and MVD. Moreover, AEG‐1 expression was correlated with clinical stage, lymphatic venous invasion, lymph nodal metastasis, tumor size, Ki67, and recurrence. Patients with AEG‐1 high‐expression showed far lower disease‐free survival (DFS) and overall survival (OS) rates than those with AEG‐1 low‐expression. For VEGF and MVD, there were similar results in these patients. Only AEG‐1 expression and tumor size were independent prognostic factors for both DFS and OS by multivariate analysis. However, the prognostic impact of tumor size was not as strong as that of AEG‐1.ConclusionsAEG‐1 expression may be related with tumor angiogenesis and progression and is a valuable prognostic factor in patients with triple‐negative breast cancer. J. Surg. Oncol. 2011; 103:184–192. © 2010 Wiley‐Liss, Inc.
Strengthening family coping resources: The feasibility of a multifamily group intervention for families exposed to trauma
Authors: Kiser Laurel J. , Donohue April , Hodgkinson Stacy , Medoff Deborah , Black Maureen M. 
Published: November 2010

AbstractFamilies exposed to urban poverty face a disproportionate risk of exposure to repeated trauma. Repeated exposures can lead to severe and chronic reactions in multiple family members with effects that ripple throughout the family system. Interventions for distressed families residing in traumatic contexts, such as low‐income, urban settings are desperately needed. This report presents preliminary data in support of Strengthening Family Coping Resources, a trauma‐focused, multifamily, skill‐building intervention. Strengthening Family Coping Resources is designed for families living in traumatic contexts with the goal of reducing symptoms of posttraumatic stress disorder and other trauma‐related disorders in children and caregivers. Results from open trials suggest Strengthening Family Coping Resources is a feasible intervention with positive effects on children's symptoms of trauma‐related distress.
Hypermethylation of apoptotic genes as independent prognostic factor in neuroblastoma disease
Authors: Grau Elena , Martinez Francisco , Orellana Carmen , Canete Adela , Yañez Yania , Oltra Silvestre , Noguera Rosa , Hernandez Miguel , Bermúdez Jose D. , Castel Victoria 
Published: November 2010

AbstractNeuroblastoma (NB) is an embryonal tumour of neuroectodermal cells, and its prognosis is based on patient age at diagnosis, tumour stage and MYCN amplification, but it can also be classified according to their degree of methylation. Considering that epigenetic aberrations could influence patient survival, we studied the methylation status of a series of 17 genes functionally involved in different cellular pathways in patients with NB and their impact on survival. We studied 82 primary NB tumours and we used methylation‐specific‐PCR to perform the epigenetic analysis. We evaluated the putative association among the evidence of hypermethylation with the most important NB prognostic factors, as well as to determine the relationship among methylation, clinical classification and survival. CASP8 hypermethylation showed association with relapse susceptibility and, TMS1 and APAF1 hypermethylation are associated with bad prognosis and showed high influence on NB overall survival. Hypermethylation of apoptotic genes has been identified as a good candidate of prognostic factor. We propose the simultaneous analysis of hypermethylation of APAF1, TMS1 and CASP8 apoptotic genes on primary NB tumour as a good prognostic factor of disease progression. Mol. Carcinog. © 2010 Wiley‐Liss, Inc.
The association ofXPCpolymorphisms and tea drinking with colorectal cancer risk in a Chinese population
Authors: Wu Yinyin , Jin Mingjuan , Liu Bing , Liang Xia , Yu Yunxian , Li Qilong , Ma Xinyuan , Yao Kaiyan , Chen Kun 
Published: November 2010

AbstractThe xeroderma pigmentosum complementation group C (XPC) is responsible for removal of bulky helix‐distorting DNA lesions. Several polymorphisms ofXPCgene may modulate the colorectal cancer (CRC) susceptibility. We assessed the association ofXPCLys939Gln (A/C), Ala499Val (C/T), and PAT (−/+) polymorphisms with CRC risk in a population‐based case–control study which included 421 CRC patients and 845 controls. For Lys939Gln, the CC genotype was associated with a significantly increased risk of CRC (odds ratio (OR) = 1.5; 95% confidence interval (CI) = 1.0−2.2) compared with the AA genotype. The subjects with PAT +/+ genotype had a significantly increased risk of CRC (OR = 1.5; 95% CI = 1.0−2.3), compared with those with PAT−/− genotype. Though no significant association between Ala499Val and CRC risk was observed, we found that individuals carrying the CT + TT genotypes showed a significantly decreased risk of rectal cancer (OR = 0.7; 95% CI = 0.5−1.0). Additionally, the haplotype C + C was associated with a significantly increased CRC risk (OR = 1.3; 95% CI = 1.0−1.6), compared with the most common haplotype A‐T. Further, individuals with four or more risk alleles exhibited a significantly increased risk of CRC (OR = 1.4; 95% CI = 1.0−2.0), with a significant gene‐dosage effect (Pfor trend = 0.038). Besides, never tea drinking was associated with a significantly increased risk of CRC (OR = 2.3; 95% CI = 1.7−3.3). Our results suggest that theXPCpolymorphisms may modulate CRC susceptibility independently or jointly, and tea drinking has a protective effect on CRC. Mol. Carcinog. © 2010 Wiley‐Liss, Inc.
The p53‐, Bax‐ and p21‐dependent inhibition of colon cancer cell growth by 5‐hydroxy polymethoxyflavones
Authors: Qiu Peiju , Guan Huashi , Dong Ping , Li Shiming , Ho Chi‐Tang , Pan Min‐Hsiung , McClements David Julian , Xiao Hang 
Published: November 2010

AbstractScope: Previously, we reported that 5‐hydroxy polymethoxyflavones (5OH‐PMFs) isolated from orange, namely 5‐hydroxy‐6,7,8,3′,4′‐pentamethoxyflavone, 5‐hydroxy‐3,6,7,8,3′,4′‐hexamethoxyflavone (5HHMF) and 5‐hydroxy‐6,7,8,4′‐tetramethoxyflavone (5HTMF), potently induced apoptosis and cell‐cycle arrest in multiple human colon cancer cells. Herein, using isogenic variants of HCT116 human colon cancer cells, we investigated the effects of p53, Bax and p21 on the apoptosis and cell‐cycle arrest induced by different 5OH‐PMFs.Methods and results: Annexin V/PI co‐staining assay demonstrated that 5HHMF and 5HTMF significantly induced apoptosis in HCT116 (p53+/+) cells but not in HCT116 (p53−/−) cells. Furthermore, 5HHMF and 5HTMF significantly induced apoptosis in HCT116 (Bax+/−) cells, whereas their pro‐apoptotic effects on HCT116 (Bax−/−) cells were marginal. All three 5OH‐PMFs increased G0/G1 cell population of HCT116 (p53+/+) cells, and these effects were abolished in HCT116 (p53−/−) and HCT116 (p21−/−) cells. Immunoblotting analysis showed that 5HHMF and 5HTMF increased the levels of cleaved caspase‐3, cleaved PARP in both HCT116 (p53+/+) and HCT116 (Bax+/−) cells and these effects were much weaker in HCT116 (p53−/−) and HCT116 (Bax−/−) cells.Conclusion: Our results demonstrated that 5OH‐PMFs, especially 5HHMF and 5HTMF, induce apoptosis and cell‐cycle arrest by p53‐, Bax‐ and p21‐dependent mechanism.
Perturbation analysis for the sign functions of regular matrix pairs
Authors: Chen Xiao Shan , Li Wen , Ching Wai‐Ki 
Published: November 2010

AbstractThis paper is devoted to the perturbation analysis for the matrix sign functions of the regular matrix pair. The first‐order perturbation bounds and the global perturbation bounds are obtained. The results are illustrated by some numerical examples. Copyright © 2010 John Wiley & Sons, Ltd.