bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combination of anodal tDCS of the cerebellum with a goal-oriented motor training to treat cervical dystonia: a pilot case series
Authors: Bleton Jean-Pierre , Cossé Charlotte , Caloc’h Tiphanie , Suarez Moreno Alcira , Diverres Elisabeth , Derkinderen Pascal , Nizard Julien , Lefaucheur Jean-Pascal , Nguyen Jean-Paul 
Published: April 2024

BackgroundTranscranial Direct Current Stimulation (tDCS) of the cerebellum shows promise for the treatment of dystonia. Specific motor rehabilitation programs have also been developed in this context. However, the combination of these two approaches has not yet been evaluated to determine their therapeutic potential.MethodsWe report a series of 5 patients with cervical dystonia (CD) poorly controlled by botulinum toxin injections. They were initially treated by a protocol of repeated daily sessions (for 3 or 5 days) of cerebellar anodal tDCS (cer-atDCS) applied alone. In a second time, additional protocols of cer-atDCS were performed in combination with a program of goal-oriented motor training exercises (Mot-Training), specifically developed for the treatment of CD. The clinical impact of the procedures was assessed on the Toronto Western Spasmodic Torticollis Rating Scale (TWSTRS).ResultsCompared to baseline, the maximum percentage of TWSTRS total score improvement was 37% on average after cer-atDCS performed alone (p = 0.147, not significant) and 53% on average after cer-atDCS combined with Mot-Training (p = 0.014, significant). The TWSTRS pain and functional handicap subscores also improved after the combined protocol. A score of (+3) to (+5) was rated on the TWSTRS response scale after cer-atDCS performed alone or the combined protocol, corresponding to a moderate to striking improvement on dystonia and pain. This improvement lasted longer after the combined protocol than after cer-atDCS alone (3.4 vs. 1.4 months on average, p = 0.011).ConclusionThe combination of cer-atDCS with Mot-Training produced a greater and more prolonged improvement than the application of cer-atDCS alone. Such a combined therapeutic procedure is easy to perform and opens important perspectives in the long-term treatment of CD. These results remain to be confirmed by a randomized sham-controlled trial on a larger sample.
Case Report: A rare treatable metabolic syndrome (Brown-Vialetto-Van Laere syndrome) masquerading as chronic inflammatory demyelinating polyneuropathy from Saudi Arabia
Authors: Kentab Amal Y. , Alsalloum Yara , Labani Mai , Hudairi Abrar , Hamad Muddathir H. , Jamjoom Dima Z. , Alwadei Ali H. , Alhammad Reem M. , Bashiri Fahad A. 
Published: April 2024

BackgroundBrown-Vialetto-Van Laere (BVVL) syndrome is an extremely rare autosomal recessive progressive motoneuron disease that is caused by a defect in the riboflavin transporter genes SLC52A2 and SLC52A3. BVVL syndrome has a variable age of presentation, and it is characterized by progressive auditory neuropathy, bulbar palsy, stridor, muscle weakness, and respiratory compromise secondary to diaphragmatic and vocal cord paralysis. BVVL syndrome has a poor prognosis in the absence of treatment, including morbidity with quadriparesis and sensorineural hearing loss, with mortality in the younger age group. Early administration of riboflavin is associated with prolonged survival, low morbidity, and reversal of some clinical manifestations.Case presentationWe describe an 18-month-old male infant with progressive pontobulbar palsy, loss of developmental milestones, and a clinical picture suggestive of chronic inflammatory demyelinating neuropathy. A nerve conduction study revealed axonal neuropathy, while molecular analysis revealed a homozygous mutation in one of the riboflavin transporter genes, SLC52A3, confirming BVVL syndrome. The patient needed long-term respiratory support and a gastrostomy tube to support feeding. With high-dose riboflavin supplementation, he experienced moderate recovery of motor function.ConclusionThis report highlights the importance of considering BVVL syndrome in any patient who presents with the clinical phenotype of pontobulbar palsy and peripheral axonal neuropathy, as early riboflavin treatment may improve or halt disease progression, thus reducing the associated mortality and morbidity.
Daily-life stress reactivity and recovery following virtual-reality-based cognitive behavioral therapy in patients with a psychotic disorder
Authors: van der Stouwe Elisabeth C. D. , Booij Sanne H. , Geraets Chris N. W. , Pot-Kolder Roos M. C. A. , Kuranova Anna , van der Gaag Mark , Veling Wim 
Published: April 2024

IntroductionStudies have consistently demonstrated increased stress sensitivity in individuals with psychosis. Since stress sensitivity may play a role in the onset and maintenance of psychosis, this could potentially be a promising target for treatment. The current study was the first to investigate whether reactivity to and recovery from daily-life stressors in psychosis change in response to treatment, namely virtual-reality-based cognitive behavioral therapy (VR-CBT).Methods116 patients were randomized to either VR-CBT or the waiting list control group (WL). Pre-treatment and post-treatment participants completed a diary ten times a day during six to ten days. Multilevel analyses were used to model the time-lagged effects of daily stressful events on negative affect (NA) and paranoia symptoms to examine reactivity and recovery.ResultsThere was a significant difference in NA reactivity. VR-CBT showed higher NA at post-treatment compared to pre-treatment than WL (bpre=0.14; bpost=0.19 vs bpre=0.18; bpost=0.14). There was a significant difference in NA recovery and paranoia recovery between the groups at lag 1: VR-CBT showed relatively lower negative affect (bpre=0.07; bpost=-0.06) and paranoia (bpre= 0.08; bpost=-0.10) at post-treatment compared to pre-treatment than WL (bpre=0.08; bpost=0.08; bpre=0.04; bpost=0.03).ConclusionNegative affect and paranoia recovery improved in response to treatment. Increased NA reactivity may be explained by a decrease in safety behavior in the VR-CBT group. The discrepancy between reactivity and recovery findings may be explained by the inhibitory learning theory that suggests that an original threat reaction may not erase but can be inhibited as a consequence of exposure therapy.
Eight weeks of high-intensity interval vs. sprint interval training effects on overweight and obese adolescents carried out during the cool-down period of physical education classes: randomized controlled trial
Authors: González-Gálvez Noelia , Soler-Marín Antonio , Abelleira-Lamela Tomás , Abenza-Cano Lucia , Mateo-Orcajada Adrián , Vaquero-Cristóbal Raquel 
Published: April 2024

ObjectiveThe aim of this study was to evaluate the effect of sprint interval training (SIT) and [high intensive interval training (HIIT)] carried out during the cool-down period of the physical education classes on body composition, blood pressure variables (BP) and pulse rate (PR), and cardiorespiratory fitness of adolescents who are overweight and obese, and to compare the differences in enjoyment in response to SIT vs. HIIT.MethodsFor this randomized controlled trial, forty-five adolescents were recruited from a high school and were randomly placed into three groups. SIT and HIIT trained for 8 weeks, twice a week, for 12 min/session. Experimental group (EG) 3 was the control, and they maintained their regular physical education class schedule. The SIT group performed 6 sets of 60 s of work (90-95%HRmax) / 60 s of rest (50-55%HRmax), and the HIIT group performed 3 sets of 2 min of work (80-85%HRmax) / 2 min of rest (50-55%HRmax).ResultsBoth experimental groups showed a significant improvement in fat mass (FM) (%) and trunk FM (kg). In addition, EG2 reported a significance improvement in lean mass (kg), blood pressure BP (mmHG), systolic blood pressure (SBP) (mmHg), diastolic blood pressure (DBP) (mmHg), PR (bpm), and VO2max (ml/kg/min).ConclusionThe present study found that a HIIT protocol performed during the cool-down period of the physical education classes generated adaptations such as improvement in body composition, BP variables and PR, and cardiorespiratory fitness, in overweight and obese adolescents. In contrast, the group of overweight and obese adolescents who performed SIT showed limited benefits, with changes in fat mass only.
Task-specific resistance training adaptations in older adults: comparing traditional and functional exercise interventions
Authors: Pagan Jason I. , Bradshaw Bethany A. , Bejte Brisilda , Hart Jordan N. , Perez Vanjeliz , Knowles Kevan S. , Beausejour Jonathan P. , Luzadder Marc , Menger Reed , Osorio Carlos , Harmon Kylie K. , Hanney William J. , Wilson Abigail T. , Stout Jeffrey R. , Stock Matt S. 
Published: April 2024

Muscle strength declines ∼3% per year after the age of 70. Resistance training guidelines for older adults are often based on free-weight and machine exercises, which may be inaccessible and lack carryover to activities of daily living. We tested the hypothesis that resistance training adaptations in older adults are task-specific. Thirty adults (8 males, 22 females; mean age = 71 years) were randomly assigned to participate in 6 weeks of supervised, high-intensity resistance training (twice per week) utilizing free-weight and machine exercises (traditional) versus functional activities that were overloaded with a weighted vest (functional). Participants were thoroughly familiarized with the exercises and testing prior to beginning the study. Major outcome measures included assessments of functional performance, five-repetition maximum strength, isometric knee extensor force, and quadriceps muscle size. Physical activity and nutrition were monitored. The study results demonstrate that the magnitude of improvement within a given outcome was largely dependent on group assignment, with greater improvements in gait speed and the timed-up-and-go in the functional group, but 2-3× greater five repetition maximum strength improvements for the trap bar deadlift, leg press, and leg extension following traditional resistance training. Both groups showed improvements in isometric knee extensor force and muscle size, suggesting that some aspects of the observed adaptations were generic, rather than specific. Overall, these novel findings suggest that, among older adults, 1) resistance training adaptations exhibit a high degree of task specificity and 2) significant improvements in functional outcomes can be achieved with the use of a weighted vest.
Heterogeneity measurement of the impact of the rural land three rights separation policy on farmers’ income based on DID model
Authors: Hu Shanshan , Fu Zhaogang , Chen Zhen , Xue Qingyi 
Published: April 2024

IntroductionAs is well known, the policy of separating three rights is another important milestone in China’s land system reform. This policy has been in effect for 10 years and is of great significance to the livelihoods of rural families. In the implementation of policies, some farmers have obtained more land management rights, but some farmers have temporarily lost their land management rights. Existing research has shown that there is no consensus on the effect rural land three rights separation on increasing farmers’ income, especially in terms of heterogeneity research, which is more scattered.MethodsWe will use the latest national fixed observation point data from the Ministry of Agriculture and Rural Affairs from 2011 to 2020, as well as data from Peking University Treasure Database, West Lake Law Library Database, China Statistical Yearbook, and China Rural Statistical Yearbook. This paper matched the unbalanced panel data of 9,846 rural household samples from 30 provinces except Hong Kong, Macao, Taiwan and Xizang, and conducted an empirical study using the multi time point DID method.ResultThe overall result shows that the policy of three rights separation of rural land can improve the income of farmers, and the impact is more obvious after the promulgation of relevant laws. From the perspective of farmers’ heterogeneity, farmers with more training, food crop planting farmers, and farmers with relatively large land scales are more significantly affected by the policy’s income increase effect.DiscussionScholars have yet to find a good explanation for how the rural land three rights separation affects farmers’ income. In this article, it appears that the three rights separation policy has promoted the increase of farmers’ income through intermediary mechanisms such as investment level, credit level, and non-agricultural employment level.
Acute intoxication with diisopropylfluorophosphate promotes cellular senescence in the adult male rat brain
Authors: Tsai Yi-Hua , González Eduardo A. , Grodzki Ana C. G. , Bruun Donald A. , Saito Naomi H. , Harvey Danielle J. , Lein Pamela J. 
Published: April 2024

Acute intoxication with high levels of organophosphate (OP) cholinesterase inhibitors can cause cholinergic crisis, which is associated with acute, life-threatening parasympathomimetic symptoms, respiratory depression and seizures that can rapidly progress to status epilepticus (SE). Clinical and experimental data demonstrate that individuals who survive these acute neurotoxic effects often develop significant chronic morbidity, including behavioral deficits. The pathogenic mechanism(s) that link acute OP intoxication to chronic neurological deficits remain speculative. Cellular senescence has been linked to behavioral deficits associated with aging and neurodegenerative disease, but whether acute OP intoxication triggers cellular senescence in the brain has not been investigated. Here, we test this hypothesis in a rat model of acute intoxication with the OP diisopropylfluorophosphate (DFP). Adult male Sprague-Dawley rats were administered DFP (4 mg/kg, s.c.). Control animals were administered an equal volume (300 µL) of sterile phosphate-buffered saline (s.c.). Both groups were subsequently injected with atropine sulfate (2 mg/kg, i.m.) and 2-pralidoxime (25 mg/kg, i.m.). DFP triggered seizure activity within minutes that rapidly progressed to SE, as determined using behavioral seizure criteria. Brains were collected from animals at 1, 3, and 6 months post-exposure for immunohistochemical analyses of p16, a biomarker of cellular senescence. While there was no immunohistochemical evidence of cellular senescence at 1-month post-exposure, at 3- and 6-months post-exposure, p16 immunoreactivity was significantly increased in the CA3 and dentate gyrus of the hippocampus, amygdala, piriform cortex and thalamus, but not the CA1 region of the hippocampus or the somatosensory cortex. Co-localization of p16 immunoreactivity with cell-specific biomarkers, specifically, NeuN, GFAP, S100β, IBA1 and CD31, revealed that p16 expression in the brain of DFP animals is neuron-specific. The spatial distribution of p16-immunopositive cells overlapped with expression of senescence associated β-galactosidase and with degenerating neurons identified by FluoroJade-C (FJC) staining. The co-occurrence of p16 and FJC was positively correlated. This study implicates cellular senescence as a novel pathogenic mechanism underlying the chronic neurological deficits observed in individuals who survive OP-induced cholinergic crisis.
Survey of tick-borne pathogens in grazing horses in Kyrgyzstan: phylogenetic analysis, genetic diversity, and prevalence of Theileria equi
Authors: Altay Kursat , Erol Ufuk , Sahin Omer Faruk , Ulucesme Mehmet Can , Aytmirzakizi Ayperi , Aktas Munir 
Published: April 2024

IntroductionTick-borne pathogens (TBP) are an important group of organisms that can affect animals and humans all over the world. Equine piroplasmosis (EP), caused by Theileria equi and Babesia caballi, is considered one of the most important tick-borne diseases and can cause significant clinical symptoms and mortality in horses. Moreover, EP plays a restrictive role in international horse traditions and transportation. Although these species can cause similar symptoms, there are different 18S rRNA genotypes of T. equi (five genotypes) and B. caballi (three genotypes). Besides piroplasma species, Anaplasma and hemotropic mycoplasmas (HM) are known as other important tick-borne pathogens reported in horses.MethodsIn this study, we investigated the presence, prevalence, genetic diversity, and phylogenetic analyses of TBPs using PCRs and DNA sequencing in grazing horses in Kyrgyzstan. For these purposes, a total of 311 blood samples were collected from Chuy, Issyk-Kul, Naryn, Osh, Talas, and Jalal-Abad.ResultsDNA amplification of TBP revealed that 23 (7.40%) out of 311 samples were found to be positive for T. equi. However, B. caballi, HM, A. phagocytophilum, and A. capra were not detected in this study. The infection rate of T. equi was higher in males (8.11%) than in females (6.35%) (p=0.2880) and in those older than 5 years (9.02%) than in the 1-4 age group (6.35%) (p=0.1950). Phylogenetic analysis of 18S rRNA revealed that A and E genotypes of T. equi have circulated in grazing horses in Kyrgyzstan.DiscussionInformation about the genetic diversity of T. equi is important for understanding the population dynamics of the species and developing effective control strategies against this pathogen. This is the first molecular investigation of A. capra in horses in Kyrgyzstan. Although this pathogen has been detected in different hosts in Kyrgyzstan, it was not detected in this study. However, considering the wide host spectrum of A. capra, it is thought that more large-scale studies are needed to understand the effect of horses on the epidemiology of this pathogen.
Different Sensitivity of Flower-Visiting Diptera to a Neonicotinoid Insecticide: Expanding the Base for a Multiple-Species Risk Assessment Approach
Authors: Henriques Martins Cátia Ariana , Azpiazu Celeste , Bosch Jordi , Burgio Giovanni , Dindo Maria Luisa , Francati Santolo , Sommaggio Daniele , Sgolastra Fabio 
Published: April 2024

Insects play an essential role as pollinators of wild flowers and crops. At the same time, pollinators in agricultural environments are commonly exposed to pesticides, compromising their survival and the provision of pollination services. Although pollinators include a wide range of species from several insect orders, information on pesticide sensitivity is mostly restricted to bees. In addition, the disparity of methodological procedures used for different insect groups hinders the comparison of toxicity data between bees and other pollinators. Dipterans are a highly diverse insect order that includes some important pollinators. Therefore, in this study, we assessed the sensitivity of two hoverflies (Sphaerophoria rueppellii, Eristalinus aeneus) and one tachinid fly (Exorista larvarum) to a neonicotinoid insecticide (Confidor®, imidacloprid) following a comparative approach. We adapted the standardized methodology of acute contact exposure in honey bees to build dose–response curves and calculate median lethal doses (LD50) for the three species. The methodology consisted in applying 1 µL of the test solution on the thorax of each insect. Sphaerophoria rueppelli was the most sensitive species (LD50 = 10.23 ng/insect), and E. aeneus (LD50 = 18,176 ng/insect) the least. We then compared our results with those available in the literature for other pollinator species using species sensitivity distribution (SSD). Based on the SSD curve, the 95th percentile of pollinator species would be protected by a safety factor of 100 times the Apis mellifera endpoint. Overall, dipterans were less sensitive to imidacloprid than most bee species. As opposed to most bee species, oviposition and fecundity of many dipteran species can be reliably assessed in the laboratory. We measured the number of eggs laid following exposure to different insecticide doses and assessed the potential trade-off between oviposition and survival through the sublethal sensitivity index (SSI). Exposure to imidacloprid had a significant effect on fecundity, and SSI values indicated that oviposition is a sensitive endpoint for the three dipteran species tested. Future studies should integrate this information related to population dynamics in simulation models for environmental risk assessment.
Advance care planning before and during the COVID-19 pandemic: an observational cohort study of 73 675 patients’ records
Authors: McFarlane Philippa G , Bunce Catey , Sleeman Katherine E , Orlovic Martina , Koffman Jonathan , Rosling John , Bearne Alastair , Powell Margaret , Riley Julia , Droney Joanne 
Published: April 2024

Background
Advance care planning (ACP) was encouraged by policymakers throughout the COVID-19 pandemic. Little is known about use of ACP during this time.

Aim
To compare use of ACP before and during the COVID-19 pandemic.

Design & setting
Retrospective, observational cohort study comparing the creation, use, and content of Electronic Palliative Care Coordination System (EPaCCS) records in London. Individuals aged ≥18 years with a Coordinate My Care record, created and published in the pre-pandemic period (1 January 2018–31 December 2019), wave 1 (W1; 20 March 2020–4 July 2020), interwave (IW; 5 July 2020–30 September 2020), and wave 2 (W2; 1 October 2020–5 March 2021).

Method
Patient demographics and components of ACP were compared using descriptive and comparative statistics.

Results

In total, 73 675 records were included; 35 108 pre-pandemic, 21 235 W1, 6323 IW, and 9925 W2 (
n
= 1084 records not stratified as created and published in different periods). Most records were created in primary care (55.6% pre-pandemic, 75.5% W1, and 47.7% W2). Compared with the pre-pandemic period, the average weekly number of records created increased by 296.9% W1 (
P
<0.005), 35.1% IW, and 29.1% W2 (
P
<0.005). Patients with records created during the pandemic were younger (60.8% aged ≥80 years W1, 57.5% IW, 59.3% W2, 64.9% pre-pandemic [
P
<0.005]). Patients with records created in W1 had longer estimated prognoses at record creation (73.3% had an estimated prognosis of ≥1 year W1 versus 53.3% pre-pandemic [
P
<0.005]) and were more likely to be 'for resuscitation' (38.2% W1 versus 29.8% pre-pandemic [
P
<0.005]).

Conclusion
During the COVID-19 pandemic increased ACP activity was observed, especially in primary care, for younger people and those not imminently dying. Further research is needed to identify training and planning requirements as well as organisational and system changes to support sustained high-quality ACP within primary care.
ASUHAN KEBIDANAN DENGAN FOKUS INTERVENSI PEMBERIAN TERAPI UAP DAUN KAYU PUTIH (MELALEUCA LEUCADENDRA) PADA BALITA SAKIT TERHADAP GANGGUAN ISPA DI PUSKESMAS TOROH 1
Authors: Vrewulan Dari Vera , Kumalasari Nurya , Himawati Laily 
Published: April 2024

Background: Acute respiratory infection (ARI) is an acute infection involving the upper and lower respiratory tract organs. This infection is caused by viruses, fungi and bacteria. , Acute Respiratory Infection ARI is the cause of 15% of under-five deaths, which is estimated to be 922,000. While in Indonesia in 2017 there was an increase of 63.45% of the number of under-five deaths 0.16% higher than in 2014 which was only 0.08%. The incidence rate for toddlers exposed to ISPA in Central Java province is 3.6% (KEMENKES, 2017).Methodology: The research design that the researchers used was a case study research design. This type of descriptive case study research used a participatory observation method. The subjects in this study were toddlers aged 3 years and 9 months. Results: research results in the case of An. O, aged 3 years and 9 months with a respiratory system disorder, the mother said that her child had no runny nose and no nose discharge, no redness on the outside of the nose and a good appetite Conclusion: there is a decrease in respiratory disorders in toddlers after being given eucalyptus leaf steam therapy.Keywords: Eucalyptus leaf steam therapy, ARI disorders, Toddlers