bluuebunny/crossref_metadata_2025_split huggingface dataset:
Reinforced Portland cement porous scaffolds for load‐bearing bone tissue engineering applications
Authors: Higuita‐Castro Natalia , Gallego‐Perez Daniel , Pelaez‐Vargas Alejandro , García Quiroz Felipe , Posada Olga M. , López Luis E. , Sarassa Carlos A. , Agudelo‐Florez Piedad , Monteiro Fernando J. , Litsky Alan S. , Hansford Derek J. 
Published: November 2011

AbstractModified Portland cement porous scaffolds with suitable characteristics for load‐bearing bone tissue engineering applications were manufactured by combining the particulate leaching and foaming methods. Non‐crosslinked polydimethylsiloxane was evaluated as a potential reinforcing material. The scaffolds presented average porosities between 70 and 80% with mean pore sizes ranging from 300 μm up to 5.0 mm. Non‐reinforced scaffolds presented compressive strengths and elastic modulus values of 2.6 and 245 MPa, respectively, whereas reinforced scaffolds exhibited 4.2 and 443 MPa, respectively, an increase of ∼62 and 80%. Portland cement scaffolds supported human osteoblast‐like cell adhesion, spreading, and propagation (t = 1–28 days). Cell metabolism and alkaline phosphatase activity were found to be enhanced at longer culture intervals (t ≥ 14 days). These results suggest the possibility of obtaining strong and biocompatible scaffolds for bone repair applications from inexpensive, yet technologically advanced materials such as Portland cement. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 100B: 501–507, 2012.
Ab Initio direct classical trajectory investigation on the SN2 reaction of F− with NH2F: Nonstatistical central barrier recrossing dynamics
Authors: Yu Feng 
Published: November 2011

AbstractThe bimolecular nucleophilic substitution (SN2) reaction of Fa− with NH2Fb has been investigated with the ab initio direct classical trajectory method. According to our trajectory calculations, a dynamic behavior of nonstatistical central barrier recrossing is revealed. Among the 64 trajectories calculated in this work, 45 trajectories follow the dynamic reaction pathways as assumed by statistical theory and other 19 trajectories with central barrier recrossings are nonstatistical. For the nonstatistical trajectories, the central barrier recrossings may originate from the inefficient kinetic energy transfer from the intramolecular modes of the NH2Fa moiety in the dynamic Fb−…HNHFa complex to the intermolecular modes of the dynamic Fb−…HNHFa complex on the exit‐channel potential energy surface. With respect to the dynamic behavior of the nonstatistical central barrier recrossing, the statistical theories such as the Rice‐Ramsperger‐Kassel‐Marcus and transition state theories without further corrections cannot be used to model the reaction kinetics for this SN2 reaction. © 2011 Wiley Periodicals, Inc. J Comput Chem, 2012
A computational study on the hydrogen adsorption capacity of various lithium—Doped boron hydrides
Authors: Pan Sudip , Giri Santanab , Chattaraj Pratim K. 
Published: November 2011

AbstractAn aromatic boron hydride B3H32– and its various Li/Li+ doped isomers have been studied at the B3LYP/6‐311+G(d) and M06/6‐311+G(d) levels of theory to assess their hydrogen storage potential. Different types of interaction energies, reaction enthalpies and reaction electrophilicities associated with the hydrogen adsorption process suggest that B3H32– itself and some of its Li—decorated analogues may turn out to be effective hydrogen storage material. Nucleus independent chemical shift and conceptual density functional theory based reactivity descriptors lend additional support. The temperature–pressure phase diagram identifies the temperature–pressure zone where the reaction Gibbs free energy for the hydrogen adsorption is negative making it a thermodynamically feasible process. © 2011 Wiley Periodicals, Inc. J Comput Chem, 2012
The Composites of Cationic Polyelectrolyte and Glutathione‐capped Quantum Dots for Selective Fluorescence Detection of Cu2+
Authors: Liu Fang‐Chen , Shen Chien‐Chih , Tseng Wei‐Lung 
Published: November 2011

AbstractA new composite of poly(diallyldimethylammonium chloride) (PDDAC) and glutathione‐capped ZnHgSe quantum dots (GSH‐QDs) has been developed for sensing Cu2+ in aqueous solution on the basis of fluorescence quenching. The formation of the composite is dominated through the electrostatic interaction between cationic PDDAC and anionic GSH‐QDs. When Cu2+ collides with PDDA/GSH‐QDs composites, Cu2+ displaces the Zn and/or Hg in the ZnHgSe QDs and forms extremely low soluble particles of CuSe onto the surface of QDs. As a result, the fluorescence intensity of QDs is quenched efficiently. Compared to GSH‐QDs, PDDA/GSH‐QDs composites exhibited better selectivity toward Cu2+ as a result of minimizing the electrostatic interaction between metal ions and the ligands. The selectivity of PDDA/GSH‐QDs composites toward Cu2+ was further improved by increasing glycine concentration and optimizing the pH of the solution. Under the optimal conditions, PDDA/GSH‐QDs composites provided the limits of detection for Cu2+ at a signal‐to‐noise ratio of 3 of 0.2 nM (∼2.0 ppt). We believe that this probe has great potential for the detection of Cu2+ in environmental waters.
Detection of Nucleoside Monophosphates through Surface‐Assisted Laser, Desorption/Ionization Mass Spectrometry Using CTAB‐Adsorbed Gold, Nanoparticles
Authors: Hsieh Yi‐Ting , Chen Wen‐Tsen , Chang Huan‐Tsung 
Published: November 2011

AbstractIn this paper, we demonstrate the determination of nucleoside monophosphates through surface‐assisted laser desorption/ionization mass spectrometry (SALDI‐MS) using hexadecyltrimethylammonium bromide (CTAB)–adsorbed gold nanoparticles (CTAB‐Au NPs) as the surface. The binding of four nucleoside monophosphates — adenosine 5′‐monophosphate (5′‐AMP), guanosine 5′‐monophosphate (5′‐GMP), uridine 5′‐monophosphate (5′‐UMP), and cytidine 5′‐monophosphate (5′‐CMP) — to the surfaces of the CTAB‐Au NPs induced their aggregation, allowing selective concentration of the four analytes from sample solutions. The analyte‐adsorbed CTAB‐Au NPs were subjected directly to SALDI‐MS measurement — that is, without mixing the analytes with organic matrices. The presence of CTAB improved the ionization efficiency of the analytes and suppressed the MS background signals arising from Au clusters. By using CTAB‐Au NPs prepared from a mixture of 0.4 μM CTAB and 1.8 nM Au NPs, this SALDI‐MS approach provided limits of detection (signal‐to‐noise ratio = 3) for 5′‐AMP, 5′‐GMP, 5′‐UMP, and 5′‐CMP of 5, 10, 25, and 25 nM, respectively. The SLADI‐MS method established was also employed for the analyses of nucleoside monophosphates in mushroom extracts and a commercial sample of monosodium glutamate. 5′‐AMP, 5′‐GMP, 5′‐UMP, and 5′‐CMP were found in the samples. This system has several advantageous features — simplicity, sensitivity, and reproducibility (shot‐to‐shot variation: < 15%) — that allow its use for the determination of the concentrations of nucleoside monophosphates in biological samples.
Synthesis and Characterization of Ultrasmall Thioglycerol Modified ZnS Quantum Dots
Authors: Ethiraj Anita S. , Wua Hui‐Fen , Gopal Judy 
Published: November 2011

AbstractThis study demonstrates a simple, rapid, low cost, and straightforward method for synthesis the thioglycerol (TG) modified ZnS QDs. These ZnS QDs were highly stable and when excited with ultraviolet light exhibited strong blue photoluminescence at ∼ 418 nm. In this study, we have successfully demonstrated the applicability of matrix assisted laser desorption ionization‐time of flight mass spectrometry (MALDI‐TOF MS) for ultrasmall size determination of the TG capped ZnS QDs (∼ 1.5 nm ± 0.03 nm). The result was found to be close to that obtained by the optical (UV/Visible) absorption method (∼ 1.2 nm ± 0.2 nm) and X‐ray diffraction technique (∼ 1.0 nm ± 0.3 nm).
Investigation of pH Effect on Gold Nanorod Synthesis
Authors: Cheng Jing , Ge Lan , Xiong Bin , He Yan 
Published: November 2011

AbstractGold nanorods (GNRs) are routinely prepared by the seed‐mediated growth method under the assistant of surfactant CTAB and it is important to understand the factors that control the formation process of GNRs. Here we investigated systematically how the pH conditions contribute to the GNR morphology and size evolution with UV‐Vis absorption spectrometry and TEM. It was found that the aspect ratio and morphology of GNRs are dependent on the pH of the growth solution. Moreover, the GNR growth pathway also varies at different pH. Our results indicated that pH affected CTAB micelle's stability and reduced its absorption ability on gold surface, which changes the gold atom deposition behavior onto the surface.
Constitutively active PTH/PTHrP receptor specifically expressed in osteoblasts enhances bone formation induced by bone marrow ablation
Authors: Ono Noriaki , Nakashima Kazuhisa , Schipani Ernestina , Hayata Tadayoshi , Ezura Yoichi , Soma Kunimichi , Kronenberg Henry M. , Noda Masaki 
Published: November 2011

AbstractBone is maintained by continuous bone formation by osteoblasts provided by proliferation and differentiation of osteoprogenitors. Parathyroid hormone (PTH) activates bone formation, but because of the complexity of cells in the osteoblast lineage, how these osteoprogenitors are regulated by PTH in vivo is incompletely understood. To elucidate how signals by PTH in differentiated osteoblasts regulate osteoprogenitors in vivo, we conducted bone marrow ablation using Col1a1‐constitutively active PTH/PTHrP receptor (caPPR) transgenic mice. These mice express caPPR specifically in osteoblasts by using 2.3 kb Col1a1 promoter and showed higher trabecular bone volume under steady‐state conditions. In contrast, after bone marrow ablation, stromal cells recruited from bone surface extensively proliferated in the marrow cavity in transgenic mice, compared to limited proliferation in wild‐type mice. Whereas de novo bone formation was restricted to the ablated area in wild‐type mice, the entire marrow cavity, including not only ablated area but also outside the ablated area, was filled with newly formed bone in transgenic mice. Bone mineral density was significantly increased after ablation in transgenic mice. Bone marrow cell culture in osteogenic medium revealed that alkaline phosphatase‐positive area was markedly increased in the cells obtained from transgenic mice. Furthermore, mRNA expression of Wnt‐signaling molecules such as LRP5, Wnt7b, and Wnt10b were upregulated after marrow ablation in bone marrow cells of transgenic mice. These results indicate that constitutive activation of PTH/PTHrP receptor in differentiated osteoblasts enhances bone marrow ablation‐induced recruitment, proliferation, and differentiation of osteoprogenitors. J. Cell. Physiol. 227: 408–415, 2012. © 2011 Wiley Periodicals, Inc.
Porous Poly(styrene‐co‐divinylbenzene) Neutral Monolith: From Design and Characterization to Reversed‐Phase Capillary Electrochromatography Applications
Authors: Lav Thanh‐Xuan , Grande Daniel , Gaillet Christine , Guerrouache Mohamed , Carbonnier Benjamin 
Published: November 2011

AbstractMacroporous cross‐linked organic polymers, based on styrene and divinylbenzene (PS‐DVB), are prepared as monolithic stationary phases for capillary electrochromatographic applications. The synthetic strategy, which relies on the semi‐interpenetrating polymer network (semi‐IPN) approach, is performed through UV‐initiated free radical copolymerization of the comonomers in the presence of poly(ϵ‐caprolactone) (PCL) within the confines of fused silica capillaries. The characterization of PS‐DVB monoliths is carried out at the different stages of the synthesis using a combination of experimental techniques, thus providing information on the chemical and porous structures. All experimental results evidence the sole role of the PCL oligomer as a porogen.
Controlled Positioning of Activated Ester Moieties on Well‐Defined Linear Polymer Chains
Authors: Kakuchi Ryohei , Zamfir Mirela , Lutz Jean‐François , Theato Patrick 
Published: November 2011

AbstractThe successful sequence‐controlled installation of an activated ester using a newly designed monomer pentafluorophenyl 4‐maleimidobenzoate is demonstrated. Pentafluorophenyl 4‐maleimidobenzoate is kinetically installed at different stages of a nitroxide‐mediated polymerization, namely, near the α‐chain end and in the middle of a PS chain. In addition, successful installation of apolar and polar functional groups is achieved via post‐polymerization functionalization, which demonstrated the versatility of the synthesis of a universal precursor for locally functionalized polymers.
Nutrigenetics of the lipoprotein metabolism
Authors: Garcia‐Rios Antonio , Perez‐Martinez Pablo , Delgado‐Lista Javier , Lopez‐Miranda Jose , Perez‐Jimenez Francisco 
Published: November 2011

AbstractIt is well known that lipid metabolism is a cornerstone in the development of the commonest important chronic diseases worldwide, such as obesity, cardiovascular disease, or metabolic syndrome. In this regard, the area of lipid and lipoprotein metabolism is one of the areas in which the understanding of the development and progression of those metabolic disorders has been studied in greater depth. Thus, growing evidence has demonstrated that while universal recommendations might be appropriate for the general population, in this area there is great variability among individuals, related to a combination of environmental and genetic factors. Moreover, the interaction between genetic and dietary components has helped in understanding this variability. Therefore, with further study into the interaction between the most important genetic markers or single‐nucleotide polymorphisms (SNPs) and diet, it may be possible to understand the variability in lipid metabolism, which could lead to an increase in the use of personalized nutrition as the best support to combat metabolic disorders. This review discusses some of the evidence in which candidate SNPs can affect the key players of lipid metabolism and how their phenotypic manifestations can be modified by dietary intake.
Role of dietary proteins and peptides in cardiovascular disease
Authors: Cam Anthony , de Mejia Elvira Gonzalez 
Published: November 2011

AbstractCardiovascular disease (CVD) is the leading cause of death for both men and women in the United States and most other countries. Therefore, a disease of such wide‐ranging impact calls for the development of multiple viable strategies for prevention. Diet plays an important role in the development of the major risk factors of CVD such as low‐grade systemic inflammation, hypertension, obesity, diabetes and atherosclerosis, the most significant. Thus, diet‐based methods of prevention would not only be more feasible, but ultimately more cost‐effective than relying on drugs to combat this condition. In recent years, peptides derived from either animal or plant sources have been found to have various bioactive properties. Nevertheless, their potential impact on inflammation and prevention of atherosclerosis has not been fully explored, particularly at the molecular level. In this review, the most current scientific information from in vitro, in vivo and clinical studies on the role of dietary proteins and peptides on CVD has been summarized and discussed.
Production of transgenic calves expressing an shRNA targeting myostatin
Authors: Tessanne K. , Golding M.C. , Long C.R. , Peoples M.D. , Hannon G. , Westhusin M.E. 
Published: November 2011

AbstractMyostatin (MSTN) is a well‐known negative regulator of muscle growth. Animals that possess mutations within this gene display an enhanced muscling phenotype, a desirable agricultural trait. Increased neonatal morbidity is common, however, resulting from complications arising from the birth of offspring with increased fetal muscle mass. The objective of the current research was to generate an attenuated MSTN‐null phenotype in a large‐animal model using RNA interference to enhance muscle development without the detrimental consequences of an inactivating mutation. To this end, we identified a series of short interfering RNAs that demonstrated effective suppression of MSTN mRNA and protein levels. To produce transgenic offspring capable of stable MSTN suppression in vivo, a recombinant lentiviral vector expressing a short hairpin RNA (shRNA) targeting MSTN for silencing was introduced into bovine fetal fibroblasts. These cells were used as nucleus donors for somatic cell nuclear transfer (SCNT). Twenty blastocysts were transferred into seven recipient cows resulting in five pregnancies. One transgenic calf developed to term, but died following delivery by Caesarean‐section. As an alternative strategy, microinjection of recombinant lentiviral particles into the perivitelline space of in vitro‐produced bovine zygotes was utilized to produce 40 transgenic blastocysts that were transferred into 14 recipient cows, resulting in 7 pregnancies. Five transgenic calves were produced, of which three expressed the transgene. This is the first report of transgenic livestock produced by direct injection of a recombinant lentivirus, and expressing transgenes encoding shRNAs targeting an endogenous gene (myostatin) for silencing. Mol. Reprod. Dev. 79: 176–185, 2012. © 2011 Wiley Periodicals, Inc.
Survivability in hierarchical telecommunications networks
Authors: Fouilhoux Pierre , Karasan Oya Ekin , Mahjoub A. Ridha , Özkök Onur , Yaman Hande 
Published: November 2011

AbstractThe survivable hierarchical telecommunications network design problem consists of locating concentrators, assigning user nodes to concentrators, and linking concentrators in a reliable backbone network. In this article, we study this problem when the backbone is 2‐edge connected and when user nodes are linked to concentrators by a point‐to‐point access network. We formulate this problem as an integer linear program and present a facial study of the associated polytope. We describe valid inequalities and give sufficient conditions for these inequalities to be facet defining. We investigate the computational complexity of the corresponding separation problems. We propose some reduction operations to speed up the separation procedures. Finally, we devise a branch‐and‐cut algorithm based on these results and present the outcome of a computational study. © 2011 Wiley Periodicals, Inc. NETWORKS, 2012
Proteomic analysis of rat microglia establishes a high‐confidence reference data set of over 3000 proteins
Authors: Bell‐Temin Harris , Barber David S. , Zhang Ping , Liu Bin , Stevens Stanley M. 
Published: November 2011

AbstractHighly aggressively proliferating immortalized (HAPI) microglial cells have been used as an in vitro model for investigating key microglial functions including inflammatory, neurotoxic, and phagocytic activities. Through the use of offline strong cation‐exchange fractionation followed by inline reversed‐phase chromatographic separation and tandem mass spectrometric analysis on a hybrid linear ion trap‐Orbitrap instrument, the HAPI microglial proteome was characterized to a depth of 3006 unique protein groups. Upon bioinformatic analysis of the HAPI proteome data set, enrichment was observed for processes relevant to microglial function including those associated with immune system response. This study marks the most comprehensive reference data set generated to date for the rat microglial proteome.
Hepatitis C core Ag and its clinical applicability: Potential advantages and disadvantages for diagnosis and follow‐up?
Authors: Hosseini‐Moghaddam SM , Iran‐Pour E. , Rotstein C. , Husain S. , Lilly L. , Renner E. , Mazzulli T. 
Published: November 2011

SUMMARYChronic hepatitis C virus (HCV) infection which is often a silent disease has resulted in a global epidemic. The diagnosis of hepatitis C virus often requires confirmation with molecular techniques such as the polymerase chain reaction for detection of HCV RNA. Following laboratory confirmation of the diagnosis, molecular techniques are routinely used to monitor HCV RNA levels, particularly in those undergoing treatment. Unfortunately, molecular tests are relatively expensive and their cost may be prohibitive in the developing world.Several studies have investigated the applicability of the hepatitis C core Ag (HCVcAg), as a substitute for measuring HCV RNA levels. In this review, we provide an overview of the major findings of these studies focused on the utility of measuring HCVcAg antigen levels in the clinical setting. Overall, measuring HCVcAg levels is associated with several advantages and disadvantages. It may be useful in different clinical settings for monitoring HCV patients after obtaining an initial baseline HCV RNA result. Copyright © 2011 John Wiley & Sons, Ltd.
Epoxy‐functionalized surfaces for microarray applications: surface chemical analysis and fluorescence labeling of surface species
Authors: Funk Christian , Dietrich Paul M. , Gross Thomas , Min Hyegeun , Unger Wolfgang E. S. , Weigel Wilfried 
Published: November 2011

AbstractMethods for characterization of epoxy‐functionalized substrates used for microarray applications, prepared by silanization with 3‐glycidoxypropyltrimethoxysilane, have been developed. Contact angle measurements, X‐ray photoelectron spectroscopy, time of flight secondary ion mass spectrometry and fluorescence based methods have been applied to investigate these epoxy‐functionalized microarray substrates. The surface density of epoxy‐functionalized glass slides was investigated by fluorescence labeling of surface species utilizing Rhodamine 110 as fluorescence probe. Copyright © 2011 John Wiley & Sons, Ltd.
Software patterns catalog based on a new classification scheme
Authors: Hasso Sargon , Carlson R.C. 
Published: November 2011

SUMMARYThere are many software pattern catalogs that exist in the published literature today, but none of them uses a consistent classification technique that results in evolving and extensible pattern catalog. We have created a new classification scheme and used it as the basis for constructing a software pattern catalog. It is a uniform approach for creating, maintaining, and expanding the catalog. We present a brief overview of the classification scheme, describe the resulting catalog and the principles underlying its construction, show how to use the catalog, and demonstrate some of its benefits for software designers. Copyright © 2011 John Wiley & Sons, Ltd.
On the feedback error compensation for adaptive modulation and coding scheme
Authors: Choi Seyeong , Yang Hong‐Chuan , Alouini Mohamed‐Slim 
Published: November 2011

ABSTRACTIn this paper, we consider the effect of feedback error on the performance of the joint adaptive modulation and diversity combining (AMDC) scheme which was previously studied with an assumption of perfect feedback channels. We quantify the performance of two joint AMDC schemes in the presence of feedback error, in terms of the average spectral efficiency, the average number of combined paths, and the average bit error rate. The benefit of feedback error compensation with adaptive combining is also quantified. Selected numerical examples are presented and discussed to illustrate the effectiveness of the proposed feedback error compensation strategy with adaptive combining. Copyright © 2011 John Wiley & Sons, Ltd.