bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bacterial cellulose modified using recombinant proteins to improve neuronal and mesenchymal cell adhesion
Authors: Pértile Renata , Moreira Susana , Andrade Fábia , Domingues Lucília , Gama Miguel 
Published: December 2011

AbstractA wide variety of biomaterials and bioactive molecules have been applied as scaffolds in neuronal tissue engineering. However, creating devices that enhance the regeneration of nervous system injuries is still a challenge, due the difficulty in providing an appropriate environment for cell growth and differentiation and active stimulation of nerve regeneration. In recent years, bacterial cellulose (BC) has emerged as a promising biomaterial for biomedical applications because of its properties such as high crystallinity, an ultrafine fiber network, high tensile strength, and biocompatibility. The small signaling peptides found in the proteins of extracellular matrix are described in the literature as promoters of adhesion and proliferation for several cell lineages on different surfaces. In this work, the peptide IKVAV was fused to a carbohydrate‐binding module (CBM3) and used to modify BC surfaces, with the goal of promoting neuronal and mesenchymal stem cell (MSC) adhesion. The recombinant proteins IKVAV‐CBM3 and (19)IKVAV‐CBM3 were successfully expressed in E. coli, purified through affinity chromatography, and stably adsorbed to the BC membranes. The effect of these recombinant proteins, as well as RGD‐CBM3, on cell adhesion was evaluated by MTS colorimetric assay. The results showed that the (19)IKVAV‐CBM3 was able to significantly improve the adhesion of both neuronal and mesenchymal cells and had no effect on the other cell lineages tested. The MSC neurotrophin expression in cells grown on BC membranes modified with the recombinant proteins was also analyzed. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012
Effect of bivalirudin compared with unfractionated heparin plus abciximab on infarct size and myocardial recovery after primary percutaneous coronary intervention: The horizons‐AMI CMRI substudy
Authors: Wöhrle Jochen , Merkle Nico , Kunze Markus , Cristea Ecaterina , Mehran Roxana , Rottbauer Wolfgang , Stone Gregg W. 
Published: December 2011

AbstractBackground: Myocardial infarct size is a strong independent predictor of mortality in patients with ST‐elevation myocardial infarction (STEMI). In the Harmonizing Outcomes with RevasculariZatiON and Stents in Acute Myocardial Infarction (HORIZONS‐AMI) trial, bivalirudin compared with unfractionated heparin plus a glycoprotein IIb/IIIa inhibitor reduced cardiac mortality in STEMI patients, which was attributed to reduced major bleeding. Whether a possible reduction in infarct size with bivalirudin may have contributed to the enhanced survival with this agent is unknown. Methods: Cardiac magnetic resonance imaging was performed within 7 days and after 6 months in 51 randomized patients from a single center in HORIZONS‐AMI trial (N = 28 bivalirudin, N = 23 heparin plus abciximab). Infarct size, microvascular obstruction (MVO), left ventricular ejection fraction (LVEF), and LV end‐diastolic and end‐systolic volume indices were evaluated. Results: Infarct size was not significantly different after treatment with bivalirudin compared with heparin plus abciximab either within 7 days (median 9.3% [interquartile range 4.9%, 26.6%] vs. 20.0% [5.9%, 28.2%], P = 0.28) or at 6 months 6.7% [3.8%, 20.0%] vs. 8.2% [1.8%, 16.5%], P = 0.73). MVO was present in 28.6% versus 34.8% of patients respectively (P = 0.63). LVEF and LV volume indices also did not significantly differ between the two groups at either time period, nor were differences in myocardial recovery evident. Conclusions: In conclusion, in the HORIZONS‐AMI Cardiac magnetic resonance imaging (CMRI) substudy, cardiac magnetic resonance imaging within 7 days and at 6 months after primary percutaneous coronary intervention (PCI) did not demonstrate significant differences in infarct size, MVO, LVEF, or LV volume indices in patients treated with bivalirudin compared with unfractionated heparin plus abciximab. © 2011 Wiley Periodicals, Inc.
Helical Content of a β3‐Octapeptide in Methanol: Molecular Dynamics Simulations Explain a Seeming Discrepancy between Conclusions Derived from CD and NMR Data
Authors: Niggli Damaris A. , Ebert Marc‐Olivier , Lin Zhixiong , Seebach Dieter , van Gunsteren Wilfred F. 
Published: December 2011

AbstractConnecting experimental observables with the underlying conformational ensemble is a long‐standing problem in the structure determination of biomolecules. The simulations described in this article attempt to resolve a seeming discrepancy between the conformational features derived from measured NOE intensities, 3J‐coupling constants, and circular dichroism (CD) spectra for two β‐peptides differing in a linker between two side‐chains. Although both peptides are very similar in terms of the r−6 averaged distances between atom pairs involved in the observed NOEs, the molecular dynamics trajectories suggest why the CD spectra show a greater 314‐helical propensity for the linked, cyclic peptide than for the linear one, whereas slightly more NMR NOE peaks are observed and assigned for the latter. The nine 100 ns unrestrained simulations show better agreement with the observed experimental data than the single conformations derived from the published NMR structures by additional energy minimization with the GROMOS force field. They show why the seemingly contradictory quantities obtained by NMR and CD spectroscopy can arise from a single conformational ensemble.
Comparative study of eight cubic equations of state for predicting thermodynamic properties of alkanes
Authors: Faradonbeh Moosa Rabiei , Abedi Jalal , Harding Thomas G. 
Published: December 2011

AbstractPrecise descriptions of the thermodynamic properties of pure fluids require accurate definition of vapour–pressure and phase volumes as well as residual volumes, enthalpies and entropies. While carefully fitted multi‐parameter equations of state (EOS), such as Benedict–Webb–Rubin–Starling fulfil these requirements, cubic EOSs usually do not. On the other hand, cubic EOSs are widely used in the oil industry, due to their simplicity and reliability in most vapour–liquid equilibrium calculations. For thermal oil recovery processes and the natural gas industry, the choice of EOS becomes important for predicting thermodynamic properties, such as isobaric and isochoric heat capacities, sound velocity and the Joule–Thomson coefficient. In this study, eight cubic EOSs which most of them are used in commercial reservoir simulators are selected for evaluation of their capability in the prediction of second‐order derivative thermodynamic properties at different temperatures and pressures, using pure components frequently found in petroleum and natural gas mixtures. It is shown that none of the cubic EOSs could accurately predict all of the stated parameters, especially below the critical point. All EOSs failed to show the extrema in the derivative properties. However, among these equations the Yu–Lu and Schmidt–Wenzel EOSs were found to have more reliable predictions in most of the cases. © 2011 Canadian Society for Chemical Engineering
Detection of doping with rhGH: Excretion study with WADA‐approved kits
Authors: Jing Jing , Yang Sheng , Zhou Xinmiao , He Chunji , Zhang Lisi , Xu Youxuan , Xie Minhao , Yan Yi , Su Hao , Wu Moutian 
Published: December 2011

The detection of recombinant human growth hormone (rhGH) doping using the World Anti‐Doping Agency (WADA) approved kits is reported in this research. Twenty‐five young male students were selected and divided randomly into two groups with six belonging to the placebo and nineteen to the administration group. Thirteen volunteers in one group were administered with a Chinese preparation of rhGH while six volunteers included in the other group were given rhGH made in Switzerland. Both preparations were administered at a dose of 0.1 IU/kg body weight, one injection per day for 14 consecutive days. Blood samples were collected using WADA guidelines and all blood samples were analyzed with WADA‐approved Kits 1 and 2.The time window for detection of rhGH doping using WADA‐approved kits and criteria are discussed. Based on the comparison of the data obtained from this excretion study and from our routine (Chinese population as reference), consideration of the recent WADA criteria for rhGH AAF (Analytical Adverse Findings) is reported statistically. A comparison of data obtained from the two sample groups administered with pharmaceutical preparations, one Chinese rhGH (GenHeal®, S19990019, 1.6 mg (4 IU), Shanghai, China) obtained from prokaryotic cells and the other (Saizen®, S20080036, 1.33 mg (4 IU), Laboratoires Serone S.A., Switzerland) from eukaryotic cells is reported and did not show any significant difference for the detection of doping with rhGH. Copyright © 2011 John Wiley & Sons, Ltd.
Studies of methylhexaneamine in supplements and geranium oil
Authors: Lisi A. , Hasick N. , Kazlauskas R. , Goebel C. 
Published: December 2011

A number of supplements are now available which are sold as fat burners or pre‐workout boosters and contain stimulants which are banned in sport. Many contain methylhexaneamine under one of many pseudonyms including Geranamine, geranium oil or extract, or a number of chemical names such as 1,3‐dimethylpentylamine. This has resulted in many athletes returning an adverse finding and having sanctions imposed. Other stimulants such as caffeine, phenpromethamine, synefrine, and phenethylamines are also to be found in supplements.This communication shows that geranium oils do not contain methylhexaneamine and that products labelled as containing geranium oil but which contain methylhexaneamine can only arise from the addition of synthetic material.Since the usual dose of methylhexaneamine is large, the drug is excreted at relatively high amounts for more than 29 h, the time for which the excretion was studied. Copyright © 2011 John Wiley & Sons, Ltd.
Improbable but true: the invasive inbreeding ambrosia beetle Xylosandrus morigerus has generalist genotypes
Authors: Andersen Hanne F. , Jordal Bjarte H. , Kambestad Marius , Kirkendall Lawrence R. 
Published: December 2011

AbstractThe wide distribution and dominance of invasive inbreeding species in many forest ecosystems seems paradoxical in face of their limited genetic variation. Successful establishment of invasive species in new areas is nevertheless facilitated by clonal reproduction: parthenogenesis, regular self‐fertilization, and regular inbreeding. The success of clonal lineages in variable environments has been explained by two models, the frozen niche variation (FNV) model and the general‐purpose genotype (GPG) model. We tested these models on a widely distributed forest pest that has been recently established in Costa Rica—the sibling‐mating ambrosia beetle Xylosandrus morigerus. Two deeply diverged mitochondrial haplotypes coexist at multiple sites in Costa Rica. We find that these two haplotypes do not differ in their associations with ecological factors. Overall the two haplotypes showed complete overlap in their resource utilization; both genotypes have broad niches, supporting the GPG model. Thus, probable or not, our findings suggest that X. morigerus is a true ecological generalist. Clonal aspects of reproduction coupled with broad niches are doubtless important factors in the successful colonization of new habitats in distant regions.
Life history traits variation in heterogeneous environment: The case of a freshwater snail resistance to pond drying
Authors: Chapuis Elodie , Ferdy Jean‐Baptiste 
Published: December 2011

AbstractEcologists and population geneticists have long suspected that the diversity of living organisms was connected to the structure of their environment. In heterogeneous environments, diversifying selection combined to restricted gene flow may indeed lead to locally adapted populations. The freshwater snail, Galba truncatula, is a good model to address this question because it is present in a heterogeneous environment composed of temporary and permanent waters. In order to test the selective importance of those environments, we proposed here to measure survival of lineages from both habitats during drought episodes. To this purpose, we experimentally submitted adults and juveniles individuals from both habitats to drought. We found a difference in desiccation resistance between temporary and permanents waters only for adults. Adults from temporary habitats were found more resistant to drought. This divergence in desiccation resistance seems to explain the unexpected life history traits differences between habitats observed.
Pheromone production, male abundance, body size, and the evolution of elaborate antennae in moths
Authors: Symonds Matthew R.E. , Johnson Tamara L. , Elgar Mark A. 
Published: December 2011

AbstractThe males of some species of moths possess elaborate feathery antennae. It is widely assumed that these striking morphological features have evolved through selection for males with greater sensitivity to the female sex pheromone, which is typically released in minute quantities. Accordingly, females of species in which males have elaborate (i.e., pectinate, bipectinate, or quadripectinate) antennae should produce the smallest quantities of pheromone. Alternatively, antennal morphology may be associated with the chemical properties of the pheromone components, with elaborate antennae being associated with pheromones that diffuse more quickly (i.e., have lower molecular weights). Finally, antennal morphology may reflect population structure, with low population abundance selecting for higher sensitivity and hence more elaborate antennae. We conducted a phylogenetic comparative analysis to test these explanations using pheromone chemical data and trapping data for 152 moth species. Elaborate antennae are associated with larger body size (longer forewing length), which suggests a biological cost that smaller moth species cannot bear. Body size is also positively correlated with pheromone titre and negatively correlated with population abundance (estimated by male abundance). Removing the effects of body size revealed no association between the shape of antennae and either pheromone titre, male abundance, or mean molecular weight of the pheromone components. However, among species with elaborate antennae, longer antennae were typically associated with lower male abundances and pheromone compounds with lower molecular weight, suggesting that male distribution and a more rapidly diffusing female sex pheromone may influence the size but not the general shape of male antennae.
Synthesis of Ir[μ2‐(N‐N)]M (M = Ir and Ru) Homo‐ and Heterobimetallic Complexes through a Condensation Reaction of N‐Amino and Formyl Groups Bound to Mononuclear (ηn‐CnMen)M Units (n = 5 for M = Ir; n = 6 for M = Ru)
Authors: Nakao Keisuke , Choi Gyeongshin , Konishi Yuki , Tsurugi Hayato , Mashima Kazushi 
Published: December 2011

AbstractReactions of [{Cp*IrCl(μ‐Cl)}2] (Cp* = η5‐pentamethylcyclopentadienyl) with bidentate pyridine–imine ligands such as 2‐(1‐hydrazonoethyl)pyridine (L1‐NH2) and 4‐({[1‐(pyridine‐2‐yl)ethylidene]hydrazono}methyl)benzaldehyde (L3‐CHO), followed by salt exchange, afforded the corresponding iridium mononuclear complexes [Cp*IrCl(L1‐NH2)][PF6] (1a‐PF6) and [Cp* IrCl(L3‐CHO)][PF6] (6‐PF6) that bear N‐amino and formyl functionalities, whereas the reaction of [{Cp*IrCl(μ‐Cl)}2] with butane‐2,3‐diylidenebis(hydrazone) [L2‐(NH2)2; 2 equiv.] afforded the half‐metallocene iridium complex 2‐PF6 with two free amino groups at the 2,5‐positions of an irida‐2,5‐diazacyclopentene skeleton. Post‐functionalization of the N‐amino groups of 1a‐PF6 and 6‐PF6 was accomplished with 3,5‐dimethoxybenzaldehyde in the presence of a catalytic amount of H2SO4 in CH3CN heated at reflux. The condensation reaction proceeded to form N‐imino‐functionalized iridium mononuclear complexes 3‐PF6 and 4‐PF6. Dinuclearization of complex 1a‐PF6 was achieved by a condensation reaction of 1a‐PF6 with dialdehydes. Reaction of an N‐amino functional group of 1a‐PF6 with terephthalaldehyde and isophthalaldehyde in the presence of a catalytic amount of H2SO4 gave the corresponding homodinuclear iridium complexes m‐5‐PF6 and p‐5‐PF6 in high yield. The molecular structure of p‐5‐PF6, evaluated by X‐ray diffraction study, revealed that two {Cp*IrCl} fragments coordinated to the tetradentate linked hydrazonoethyl skeleton in an anti fashion. Furthermore, treatment of the formyl group of iridium complex 6‐PF6 with the N‐amino group of ruthenium complex 1b‐PF6 resulted in the selective formation of an Ir[μ2‐(N‐N)]Ru heterobimetallic complex (7‐PF6) under similar reaction conditions. The selectivity for the formation of the Ru–Ir heterobimetallic complex was due to the tolerance of the imine moiety to hydrolysis under acidic conditions.
Synthesis of Rhenium(I) Tricarbonyl Complexes with Carbohydrate‐Pendant Tridentate Ligands and Their Cellular Uptake
Authors: Mikata Yuji , Takahashi Kyoko , Noguchi Yuka , Naemura Masami , Ugai Anna , Itami Saori , Yasuda Keiko , Tamotsu Satoshi , Matsuo Takashi , Storr Tim 
Published: December 2011

AbstractTwelve [ReIL(CO)3]n+ complexes with various carbohydrate‐pendant ligands L have been prepared and their uptake into HeLa S3 cells were investigated. The ligand library includes: (i) glucose/galactose as the carbohydrate group; (ii) bis(2‐pyridylmethyl)amine (DPA), bis(2‐quinolylmethyl)amine (DQA), or N‐(2‐pyridylmethyl)glycine (NPG) as the metal binding component; and (iii) an ethylene chain as a linker between the metal binding site and the O/C‐glycosides. Microwave induced plasma mass spectroscopy (MIP‐MS) measurements revealed that all complexes were extensively incorporated into the HeLa cells over a 24 h period, and the DQA complexes showed the highest uptake of all the complexes in the series. However, in comparison to the corresponding Re complexes without the pendant carbohydrate functions (prepared with the related ligands LDPA, LDQA, and LNPG), only the NPG complexes exhibited carbohydrate enhanced cellular uptake. Considering their water solubility and cellular uptake properties, the NPG complexes containing an O‐glycoside group (L1 and L′1) are the best candidates for enhancing cellular uptake of metal ions. Microscopic analysis with PC‐12 cells in the presence of thefluorescent complex [Re(L′7)(CO)3]Cl, revealed that the complex stays in the cell cytosol and cannot penetrate into the nucleus.
Stereoselective Synthesis of Difunctionalized 1,3‐Dienes Containing Tin and Sulfonyl Groups by Palladium‐Catalyzed Regio‐ and Stereocontrolled Addition Reactions
Authors: Zhao Hong , Yang Weisen , Xie Shiyun , Cai Mingzhong 
Published: December 2011

AbstractUsing palladium‐catalyzed addition reactions, a terminal alkyne, an acetylenic sulfone, and a tin hydride comprise the building blocks for the construction of difunctionalized 1,3‐dienes containing tin and sulfonyl groups in an atom‐economical fashion. Terminal alkynes 1 undergo clean cis addition to acetylenic sulfones 2 in the presence of catalytic amounts of palladium acetate and tri(2,6‐dimethoxyphenyl)phosphane to give (E)‐1‐sulfonyl‐substituted 1,3‐enynes 3 in excellent yields. Hydrostannylation of 1,3‐enynes 3 with tributyltin hydride in the presence of Pd(PPh3)4 affords regio‐ and stereoselectively (1E,3E)‐1‐sulfonyl‐3‐tributylstannyl‐substituted 1,3‐dienes 4 in excellent yields.
New Optically Active 4‐Alkoxyprolinol Ethers Derived from trans‐4‐Hydroxy‐L‐proline
Authors: Friedemann Nora M. , Eustergerling Astrid , Nubbemeyer Udo 
Published: December 2011

Abstract(2S,4R)‐trans‐4‐Hydroxy‐L‐proline has been used as thechiral‐pool source in the efficient syntheses of optically active protected 4‐hydroxyprolinols. After N‐acyl protection andester formation, the first ether moiety was introduced maintaining the chiral centre adjacent to the ester. Then, reduction of the ester delivered the corresponding carbinol, which had to be alkylated selectively to avoid side reactions with the N‐protecting group. Finally, the N‐acyl function was removed to generate the target methyl and tert‐butyl ethers displaying defined substitution patterns. The so‐formed optically active 4‐alkoxyprolinol ethers can be used as core fragments in biologically active compounds or as stablechiral auxiliary subunits in suitable starting materials.
A Multicomponent Carba‐Betti Strategy to Alkylidene Heterodimers – Total Synthesis and Structure–Activity Relationships of Arzanol
Authors: Minassi Alberto , Cicione Lavinia , Koeberle Andreas , Bauer Julia , Laufer Stefan , Werz Oliver , Appendino Giovanni 
Published: December 2011

AbstractUsing the synthesis of the heterodimeric phloroglucinyl pyrone arzanol as a benchmark reaction, a carba‐version of the Betti multicomponent reaction has been developed. Capitalizing on the fluorous activation of the phenolic component and the use of iminium ions as bivalent and “transmissive” aldehyde equivalents, the reaction has been used to colonize a biologically privileged but previously inaccessible area of chemical space and investigate the structure–activity relationships of arzanol towards a series of proinflammatory targets (mPGES‐1, 5‐LO).
Recent developments and applications of EMMA in enzymatic and derivatization reactions
Authors: Hai Xin , Yang Bao‐feng , Van Schepdael Ann 
Published: December 2011

AbstractThis review provides systematic coverage of examples in the field of in‐capillary electrophorecially mediated microanalysis (EMMA). The recent developments and applications in the time period up to mid 2011 have been described, as well as relevant older papers. The basic principles and modes of in‐capillary assays have been demonstrated. An overview is also given of the various injection, separation and detection modes implemented in combination with EMMA. The review is presented in two parts mainly dealing with (i) enzymatic and (ii) derivatization or chemical reactions. Finally, the future trends of CE in performing and monitoring reactions have been drawn.
Recent advances in the application of capillary electromigration methods for food analysis and Foodomics
Authors: Castro‐Puyana María , García‐Cañas Virginia , Simó Carolina , Cifuentes Alejandro 
Published: December 2011

AbstractThe analysis of food components using capillary electromigration methods is reviewed in this work. Papers that were published from February 2009 to February 2011 are included following the previous review by Herrero et al. (Electrophoresis, 2010, 31, 205–228). The analysis of amino acids, biogenic amines, peptides, proteins, DNAs, carbohydrates, phenols, polyphenols, pigments, toxins, pesticides, vitamins, additives, small organic and inorganic ions and other compounds found in foods and beverages are reviewed, as well as those applications of CE for monitoring food interactions and food processing. The use of microchips, CE‐MS and chiral‐CE in food analysis is also discussed as well as other current and foreseen trends in this area of research including new developments in Foodomics.