bluuebunny/crossref_metadata_2025_split huggingface dataset:
Feasibility and safety report on robotic assistance for cervical pedicle screw fixation: a cadaveric study
Authors: Ryu Seungjun , Ha Byeong-Jin , Yoon Sunjin , Lee Chang Kyu , Shin Dong Ah , Kim Keung-Nyun , Yi Seong 
Published: May 2024

AbstractThis cadaveric study aimed to evaluate the safety and usability of a novel robotic system for posterior cervical pedicle screw fixation. Three human cadaveric specimens and C2-T3 were included. Freshly frozen human cadaver specimens were prepared and subjected to robot-assisted posterior cervical pedicle screw fixation using the robotic system. The accuracy of screw placement, breach rate, and critical structure violations were evaluated. The results were statistically compared with those of previous studies that used different robotic systems for cervical pedicle screw fixation. The robotic system demonstrated a high accuracy rate in screw placement. A significant number of screws were placed within predetermined safe zones. The total entry offset was 1.08 ± 0.83 mm, the target offset was 1.86 ± 0.50 mm, and the angle offset was 2.14 ± 0.77°. Accuracy rates comparable with those of previous studies using different robotic systems were achieved. The system was also feasible, allowing precise navigation and real-time feedback during the procedure. This cadaveric study validated the safety and usability of the novel robotic system for posterior cervical pedicle screw fixation. The system exhibited high precision in screw placement, and the results support the extension of the indications for robot-assisted pedicle screw fixation from the lumbar spine to the cervical spine.
A nomogram to predict mortality in patients with severe fever with thrombocytopenia syndrome
Authors: Fang Kun , Song Xuezhen , Bo Jinshuang 
Published: May 2024

AbstractSevere fever with thrombocytopenia syndrome (SFTS) is an acute infectious disease caused by a novel Bunyavirus infection with low population immunity and high mortality rate. Lacking specific therapies, the treatment measures vary with the severity of the disease, therefore, a case control study involved 394 SFTS patients was taken to determine risk factors for mortality. Comparative clinical data from the first 24 h after admission was collected through the electronic medical record system. Independent risk factors for death of SFTS were identified through univariate and multivariate binary logistic regression analyses. The results of the logistic regression were visualized using a nomogram which was created by downloading RMS package in the R program. In our study, four independent mortality risk factors were identified: advanced age(mean 70.45 ± 7.76 years), MODS, elevated APTT, and D-dimer. The AUC of the nomogram was 0.873 (0.832, 0.915), and the model passes the calibration test namely Unreliability test with P = 0.958, showing that the model's predictive ability is excellent. The nomogram to determine the risk of death in SFTS efficiently provide a basis for clinical decision-making for treatment.
Characteristics of drug resistance mutations in ART-experienced HIV-1 patients with low-level viremia in Zhengzhou City, China
Authors: Liu Jinjin , Li Chaofeng , Sun Yan , Fu Chaohong , Wei Shuguang , Zhang Xiaohua , Ma Jie , Zhao Qingxia , Huo Yuqi 
Published: May 2024

AbstractAlthough most people living with HIV (PLWH) receiving antiretroviral therapy (ART) achieve continuous viral suppression, some show detectable HIV RNA as low-level viremia (LLV) (50–999 copies/mL). Drug resistance mutations (DRMs) in PLWH with LLV is of particular concern as which may lead to treatment failure. In this study, we investigated the prevalence of LLV and LLV-associated DRMs in PLWH in Zhengzhou City, China. Of 3616 ART-experienced PLWH in a long-term follow-up cohort from Jan 2022 to Aug 2023, 120 were identified as having LLV. Of these PLWH with LLV, we obtained partial pol and integrase sequences from 104 (70 from HIV-1 RNA and 34 from proviral DNA) individuals. DRMs were identified in 44 individuals. Subtyping analysis indicated that the top three subtypes were B (48.08%, 50/104), CRF07_BC (31.73%, 33/104), and CRF01_AE (15.38%, 16/104). The proportions of nucleoside reverse transcriptase inhibitors (NRTIs), non-nucleoside reverse transcriptase inhibitors (NNRTIs), protease inhibitors (PIs), and integrase strand transfer inhibitors (INSTIs) associated DRMs were 23.83% (24/104), 35.58% (37/104), 5.77% (6/104), and 3.85% (4/104), respectively, which contributed to an overall prevalence of 42.31% (44/104). When analyzed by individual DRMs, the most common mutation(s) were V184 (18.27%, 19/104), followed by V179 (11.54%, 12/104), K103 (9.62%, 10/104), Y181 (9.62%, 10/104), M41 (7.69%, 8/104), and K65R (7.69%, 8/104). The prevalence of DRMs in ART-experienced PLWH with LLV is high in Zhengzhou City and continuous surveillance can facilitate early intervention and provision of effective treatment.
Effects of Eucalyptus wood and leaf litter on saproxylic insects in the southeastern United States
Authors: Ulyshen Michael D. , Horn Scott , Aubrey Doug , Hoebeke E. Richard , Coyle David R. 
Published: May 2024

AbstractAlthoughEucalyptusis widely planted outside its native range for timber and pulp production, the effects of these exotic plantations on biodiversity relative to native semi-natural forests or plantations of native tree species remain incompletely understood. Here, we compare the diversity of saproxylic beetles (Coleoptera) and true bugs (Hemiptera) between non-nativeEucalyptus benthamiiMaiden and Cambage (Camden white gum) and nativePinus taedaL. (loblolly pine) stands on the upper Coastal Plain of South Carolina, U.S.A. We sampled insects emerging from logs of both species placed in both stand types after 1, 2, 6, and 12 months in the field. Beetle and true bug richness and diversity were both significantly lower from eucalypt than from pine wood. Moreover, the two communities were compositionally distinct. Whereas pine supported many species of host-specific phloeoxylophagous beetles, most species collected from eucalypts were mycophagous or predatory taxa capable of utilizing a wide range of hosts. Species richness did not differ between logs placed in eucalypt vs. pine stands but Shannon’s diversity was significantly higher in the eucalypt stands, possibly due to greater sun exposure in the latter. Contrary to a previous study, we found no support for the idea that eucalypt litter reduces the diversity of saproxylic insects. Our findings add to the growing body of evidence that non-native plantations are less favorable to biodiversity than those consisting of native tree species.
Low power nanoscale S-FED based single ended sense amplifier applied in integrate and fire neuron circuit
Authors: Motaman SeyedMohamadJavad , Ghafouri Tara , Manavizadeh Negin 
Published: May 2024

AbstractCurrent advancements in neuromorphic computing systems are focused on decreasing power consumption and enriching computational functions. Correspondingly, state-of-the-art system-on-chip developers are encouraged to design nanoscale devices with minimum power dissipation and high-speed operation. This paper deals with designing a sense amplifier based on side-contacted field-effect diodes to reduce the power-delay product (PDP) and the noise susceptibility, as critical factors in neuron circuits. Our findings reveal that both static and dynamic power consumption of the S-FED-based sense amplifier, equal to 1.86 μW and 1.92 fW/GHz, are × 243.03 and × 332.83 lower than those of the conventional CMOS counterpart, respectively. While the sense-amplifier circuit based on CMOS technology undergoes an output voltage deviation of 170.97 mV, the proposed S-FED-based one enjoys a minor output deviation of 27.31 mV. Meanwhile, the superior HIGH-level and LOW-level noise margins of the S-FED-based sense amplifier to the CMOS counterparts (∆NMH = 70 mV and ∆NML = 120 mV), respectively, can ensure the system-level operation stability of the former one. Subsequent to the attainment of an area-efficient, low-power, and high-speed S-FED-based sense amplifier (PDP = 187.75 × 10–18 W s) as a fundamental building block, devising an innovative integrate-and-fire neuron circuit based on S-FED paves the way to realize a new generation of neuromorphic architectures. To shed light on this context, an S-FED-based integrate-and-fire neuron circuit is designed and analyzed utilizing a sense amplifier and feedback loop to enhance spiking voltage and subsequent noise immunity in addition to an about fourfold increase in firing frequency compared to CMOS-based ones.
Novel betaherpesviruses and gammaherpesviruses in bats from central China
Authors: Duan Shuhui , Li Zemin , Zhang Xu , Yu Xue-Jie 
Published: May 2024

AbstractHerpesviruses are large double-stranded DNA viruses that cause infections in animals and humans with a characteristic of latent infectious within specific tissues. Bats are natural hosts of variety human-infecting viruses and recently have been described as hosts for herpesviruses in several countries around the world. In this study we collected 140 insectivorous bats in the neighboring urban areas of Wuhan City, Hubei Province in the central China between 2020 and 2021. Nested PCR targeting the dpol gene sequence indicated that a total of 22 individuals (15.7% of the sample) tested positive for herpesvirus with 4 strains belonging to the genus Betaherpesvirus and the remaining 18 strains classified as Gammahersvirus. Furthermore, the herpesvirus prevalence in Rhinolophus pusillus was higher at 26.3%, compared to 8.4% in Myotis davidii. The RP701 strain from R. pusillus was the predominant gammaherpesvirus strain detected in bats, accounting for 94.4% (17/18) of all strains. The variations in γ-herpesviruses genomic sequences was evident in phylogenetic tree, where RP701 strain was clustered together with ruminant γ-herpesviruses, while MD704 strain formed a distinct clade with a hedgehog γ-herpesvirus. Four betaherpesviruses exclusively identified from M. davidii, with nucleotide identities ranging from 79.7 to 82.6% compared to known betaherpesviruses. Our study provided evidence that M. davidii can sever as natural host for β-herpesviruses, which extended the host species range. In conclusion, we found that bats from central China harbored novel β-herpesviruses and γ-herpesviruses which were phylogenetically related to ruminant γ-herpesvirus and hedgehog γ-herpesvirus. Our study indicates that bats are natural hosts of β- and γ-herpesviruses and further studies are needed to determine whether there is cross-species transmission of herpesviruses between bats and other animals, or humans.
Effect of biological shells aggregate on the mechanical properties and sustainability of concrete
Authors: Wang Xianpeng , Yu Haoxuan , Li Fulong , Sergey Nikolayevich Kovshar , Yu Haojue , Sergey Nikolaevich Leonovich , Fan Wenbing 
Published: May 2024

AbstractThe recycling bio-waste shells problem has grown more and more serious in recent years and many efforts have been made to solve this problem. One possible solution is to put these bio-shells into concrete and recycle them as building materials using the aggregate matrix concrete approach. To verify the engineering feasibility, the mechanical properties of bio-shells aggregated concrete were invested via gradient substitution rates at 10%, 30%, and 50% with a total of 78 groups of specimens in this paper. Our results show that the mechanical properties of the concrete were enhanced in maximum flexural strength and maximum compressive. Economic performance was also analyzed and found that the costs of frame-shear structure, frame structure, and tube-in-tube structure were reduced by 10.2%, 10%, and 10.3%. The carbon environmental assessment also shows superiority in the carbon reduction of a single specimen with various rates of the shell. In summary, compared with ordinary concrete materials, it is very possible to use waste bio-shells as a substitute for aggregates to develop the sustainable recycling development of concrete materials.
Lysyl oxidase-like 2 as a predictor of hepatocellular carcinoma in patients with hepatitis C virus after sustained virological response
Authors: Chida Takeshi , Ohta Kazuyoshi , Noritake Hidenao , Matsushita Masahiro , Murohisa Gou , Kageyama Fujito , Sasada Yuzo , Oyaizu Tatsuki , Tsugiki Minoru , Tamakoshi Katsutoshi , Nakajima Takeyuki , Suda Takafumi , Kawata Kazuhito 
Published: May 2024

AbstractLysyl oxidase-like 2 (LOXL2) mediates the crosslinking of extracellular collagen, reflecting qualitative changes in liver fibrosis. This study aimed to validate the utility of serum LOXL2 levels as a predictive biomarker for the development of hepatocellular carcinoma (HCC) in patients with hepatitis C virus (HCV) infection who achieved a sustained virological response (SVR). This retrospective study included 137 patients with chronic HCV infection without history of HCC development and who achieved SVR via direct-acting antiviral therapy. Median LOXL2 levels decreased significantly after SVR achievement (pre-Tx, 2.33 ng/mL; post-Tx, 1.31 ng/mL, p < 0.001). Post-Tx LOXL2 levels, fibrosis-4 index, platelet counts, Wisteria floribunda agglutinin-positive human Mac-2 binding protein levels, and alpha-fetoprotein (AFP) levels were identified as independent predictive factors for post-SVR HCC development in the univariate analysis. The incidence of post-SVR HCC development was significantly higher in patients with post-Tx LOXL2 levels ≥ 2.08 ng/mL and AFP levels ≥ 5.0 ng/mL than in patients with elevated levels of either marker or with lower marker levels. Serum LOXL2 levels can serve as a predictive biomarker for HCC development after achieving SVR. The combination of serum LOXL2 and AFP levels provides robust risk stratification for HCC development after SVR, suggesting an enhanced surveillance strategy.
Advection surface-flux balance controls the seasonal steric sea level amplitude
Authors: Hochet Antoine , Llovel William , Huck Thierry , Sévellec Florian 
Published: May 2024

AbstractAlong with the mean sea level rise due to climate change, the sea level exhibits natural variations at a large number of different time scales. One of the most important is the one linked with the seasonal cycle. In the Northern Hemisphere winter, the sea level is as much as 20 cm below its summer values in some locations. It is customary to associate these variations with the seasonal cycle of the sea surface net heat flux which drives an upper-ocean thermal expansion creating a positive steric sea level anomaly. Here, using a novel framework based on steric sea level variance budget applied to observations and to the Estimating the Circulation and Climate of the Ocean state estimate, we demonstrate that the steric sea level seasonal cycle amplitude results from a balance between the seasonal sea surface net heat flux and the oceanic advective processes. Moreover, for up to 50% of the ocean surface, surface heat fluxes act to damp the seasonal steric sea level cycle amplitude, which is instead forced by oceanic advection processes. We also show that eddies play an important role in damping the steric sea level seasonal cycle. Our study contributes to a better understanding of the steric sea level mechanisms which is crucial to ensure accurate and reliable climate projections.
Comparison of anthracycline-containing and anthracycline-free regimens in neoadjuvant HER-2 positive breast cancer treatment
Authors: Bardakci Murat , Karakas Hilal , Bayram Dogan , Avci Nilufer , Kitapli Sait , Ozen Mirac , Aslan Ferit , Koseoglu Caglar , Kadioglu Ahmet , Onur Ilknur D. , Sakalar Teoman , Buyuksimsek Mahmut , Alkan Ali , Ergun Yakup , Kaya Ali O. , Bilgin Burak , Yalcin Bulent 
Published: May 2024

AbstractWhile some clinics have adopted abbreviated neoadjuvant treatment for HER2-positive breast cancer, there remains a shortage of comprehensive clinical data to support this practice. This is a retrospective, multicenter study. A total of 142 patients were included in the study who are HER2-positive breast cancer, aged ≤ 65 years, with left ventricular ejection fraction ≥ 50%, received neoadjuvant chemotherapy and underwent surgery at 10 different oncology centers in Türkiye between October 2016 and December 2022. The treatment arms were divided into 4–6 cycles of docetaxel/trastuzumab/pertuzumab for arm A, 4 cycles of adriamycin/cyclophosphamide followed by 4 cycles of taxane/TP for arm B. There were 50 patients (35.2%) in arm A and 92 patients (64.8%) in arm B. The median follow-up of all of the patients was 19.9 months (95% CI 17.5–22.3). The 3-year DFS rates for treatment arms A and B were 90.0% and 83.8%, respectively, and the survival outcomes between the groups were similar (p = 0.34). Furthermore, the pathologic complete response rates were similar in both treatment arms, at 50.0% and 51.1%, respectively (p = 0.90). This study supports shortened neoadjuvant treatment of HER2-positive breast cancer, a common practice in some clinics.
Spacetime magnetic hopfions from internal excitations and braiding of skyrmions
Authors: Knapman Ross , Tausendpfund Timon , Díaz Sebastián A. , Everschor-Sitte Karin 
Published: May 2024

AbstractSpatial topology endows topological solitons, such as skyrmions and hopfions, with fascinating dynamics. However, the temporal dimension has so far provided a passive stage on which topological solitons evolve. Here we construct spacetime magnetic hopfions: magnetic textures in two spatial dimensions that when excited by a time-periodic drive develop spacetime topology. We uncover two complementary construction routes using skyrmions by braiding their center of mass position and by controlling their internal low-energy excitations. Spacetime magnetic hopfions can be realized in nanopatterned grids to braid skyrmions and in frustrated magnets under an applied AC electric field. Their topological invariant, the spacetime Hopf index, can be tuned by the applied electric field as demonstrated by our collective coordinate modeling and micromagnetic simulations. The principles we have introduced to actively control spacetime topology are not limited to magnetic solitons, opening avenues to explore spacetime topology of general order parameters and fields.
A comprehensive AI model development framework for consistent Gleason grading
Authors: Huo Xinmi , Ong Kok Haur , Lau Kah Weng , Gole Laurent , Young David M. , Tan Char Loo , Zhu Xiaohui , Zhang Chongchong , Zhang Yonghui , Li Longjie , Han Hao , Lu Haoda , Zhang Jing , Hou Jun , Zhao Huanfen , Gan Hualei , Yin Lijuan , Wang Xingxing , Chen Xiaoyue , Lv Hong , Cao Haotian , Yu Xiaozhen , Shi Yabin , Huang Ziling , Marini Gabriel , Xu Jun , Liu Bingxian , Chen Bingxian , Wang Qiang , Gui Kun , Shi Wenzhao , Sun Yingying , Chen Wanyuan , Cao Dalong , Sanders Stephan J. , Lee Hwee Kuan , Hue Susan Swee-Shan , Yu Weimiao , Tan Soo Yong 
Published: May 2024

AbstractBackgroundArtificial Intelligence(AI)-based solutions for Gleason grading hold promise for pathologists, while image quality inconsistency, continuous data integration needs, and limited generalizability hinder their adoption and scalability.MethodsWe present a comprehensive digital pathology workflow for AI-assisted Gleason grading. It incorporates A!MagQC (image quality control), A!HistoClouds (cloud-based annotation), Pathologist-AI Interaction (PAI) for continuous model improvement, Trained on Akoya-scanned images only, the model utilizes color augmentation and image appearance migration to address scanner variations. We evaluate it on Whole Slide Images (WSI) from another five scanners and conduct validations with pathologists to assess AI efficacy and PAI.ResultsOur model achieves an average F1 score of 0.80 on annotations and 0.71 Quadratic Weighted Kappa on WSIs for Akoya-scanned images. Applying our generalization solution increases the average F1 score for Gleason pattern detection from 0.73 to 0.88 on images from other scanners. The model accelerates Gleason scoring time by 43% while maintaining accuracy. Additionally, PAI improve annotation efficiency by 2.5 times and led to further improvements in model performance.ConclusionsThis pipeline represents a notable advancement in AI-assisted Gleason grading for improved consistency, accuracy, and efficiency. Unlike previous methods limited by scanner specificity, our model achieves outstanding performance across diverse scanners. This improvement paves the way for its seamless integration into clinical workflows.
Catalytic mechanism and kinetics of malate dehydrogenase
Authors: de Lorenzo Laura , Stack Tyler M.M. , Fox Kristin M. , Walstrom Katherine M. 
Published: May 2024

Abstract
Malate dehydrogenase (MDH) is a ubiquitous and central enzyme in cellular metabolism, found in all kingdoms of life, where it plays vital roles in the cytoplasm and various organelles. It catalyzes the reversible NAD+-dependent reduction of L-malate to oxaloacetate. This review describes the reaction mechanism for MDH and the effects of mutations in and around the active site on catalytic activity and substrate specificity, with a particular focus on the loop that encloses the active site after the substrates have bound. While MDH exhibits selectivity for its preferred substrates, mutations can alter the specificity of MDH for each cosubstrate. The kinetic characteristics and similarities of a variety of MDH isozymes are summarized, and they illustrate that the KM values are consistent with the relative concentrations of the substrates in cells. As a result of its existence in different cellular environments, MDH properties vary, making it an attractive model enzyme for studying enzyme activity and structure under different conditions.
Collaborative optimization model of industrial high‐energy consumption park under green and low‐carbon transformation
Authors: Ma Shaohua , Wang Qiwei , Nie Tong , Chen Zhe , Teng Yun 
Published: May 2024

AbstractUnder the background of green and low‐carbon transformation of industrial high‐energy‐consuming parks, in order to realize the coordinated development planning of energy and transportation under different peaking capacity requirements, this paper proposes a collaborative optimization model of industrial high‐energy‐consuming parks under green and low‐carbon. Firstly, by studying the energy balance mechanism between industrial load and new energy, an energy topology prior model of energy interaction in the next generation of industrial new energy power grid is constructed. Then, based on the probability characteristics of the equilibrium state of different regions in the high‐energy‐consuming industrial zone, combined with the prior knowledge of industrial load and low‐carbon power system, a collaborative development planning model of high‐energy‐consuming industrial parks is constructed. Finally, the proposed model is simulated. From the simulation results the authors can see the dynamic division model and solution algorithm of energy balance region established in this paper can effectively improve the energy balance ability of power grid, reduce peak shaving demand and use more renewable energy.