bluuebunny/crossref_metadata_2025_split huggingface dataset:
Life cycle assessment of Brazilian sugarcane products: GHG emissions and energy use
Authors: Seabra Joaquim E. A. , Macedo Isaias C. , Chum Helena L. , Faroni Carlos E. , Sarto Celso A. 
Published: May 2011

AbstractSugarcane is currently the main renewable energy source in Brazil. Due to the importance of the cane industry and its contribution to a wide range of biobased energy and other products, LCA studies regarding cane‐derived products are needed to assess their environmental benefits. The main objective of this work was the assessment of life cycle energy use and greenhouse gas (GHG) emissions related to cane sugar and ethanol, considering bagasse and electricity surpluses as coproducts. We performed an overall balance for the Brazilian Center‐South Region, adopting different methods to evaluate sugar and ethanol production separately. The GREET 1.8c.0 model was used for the ‘well‐to‐wheels’ calculations but adapted to the comprehensive set of Brazilian parameters that best represent the Center‐South Region. For the reference case, fossil energy use and GHG emissions related to sugar production were evaluated as 721 kJ/kg and 234 g CO2eq/kg, respectively. For the ethanol life cycle, these values were 80 kJ/MJ and 21.3 g CO2eq/MJ. Special attention was paid to the variation of some parameters among producing units based on data collected by industry. The consequent uncertainties in ethanol life cycle emissions were assessed through a Monte Carlo analysis based on assigned distribution of probability curves for eleven selected parameters and informed by partial statistical data available from industry for distribution generation. Projections were also made for 2020 scenario parameters based on the best in current class technologies and technological improvements deemed commercially possible today. Published in 2011 by John Wiley & Sons, Ltd
Site‐Specific Labeling of Proteins by Using Biotin Protein Ligase Conjugated with Fluorophores
Authors: Sueda Shinji , Yoneda Sawako , Hayashi Hideki 
Published: May 2011

AbstractBiotin protein ligase (BPL) mediates the covalent attachment of biotin to a specific lysine residue of biotin carboxyl carrier protein (BCCP). This biotinylation in Sulfolobus tokodaii is unique in that BPL forms a tight complex with the product, biotinylated BCCP, and this property was exploited for fluorescent labeling of a membrane protein. Thus, the truncated form of BCCP (BCCPΔ100, 69 residues) was fused to either the N or C terminus of the bradykinin B2 receptor (B2R). The resulting fusion proteins, BCCPΔ100–B2R and B2R–BCCPΔ100, respectively, were separately expressed in mammalian HEK293 cells, and labeled with BPL conjugated with a fluorophore: either fluorescein, DyLight549 or green fluorescent protein. The fusion proteins were biotinylated and bound to BPL, thereby giving rise to strong fluorescence along the periphery of the cell. Some were capable of binding bradykinin and an antagonist. When stimulated with the former, the receptor translocated to the cytosol; this suggests that the labeled receptor retains its integrity in terms of ligand‐binding and translocation.
Synthesis, Structural Characterization, and Pro‐apoptotic Activity of 1‐Indanone Thiosemicarbazone Platinum(II) and Palladium(II) Complexes: Potential as Antileukemic Agents
Authors: Gómez Natalia , Santos Diego , Vázquez Ramiro , Suescun Leopoldo , Mombrú Álvaro , Vermeulen Monica , Finkielsztein Liliana , Shayo Carina , Moglioni Albertina , Gambino Dinorah , Davio Carlos 
Published: May 2011

AbstractIn the search for alternative chemotherapeutic strategies against leukemia, various 1‐indanone thiosemicarbazones, as well as eight novel platinum(II) and palladium(II) complexes, with the formula [MCl2(HL)] and [M(HL)(L)]Cl, derived from two 1‐indanone thiosemicarbazones were synthesized and tested for antiproliferative activity against the human leukemia U937 cell line. The crystal structure of [Pt(HL1)(L1)]Cl.2MeOH, where L1=1‐indanone thiosemicarbazone, was solved by X‐ray diffraction. Free thiosemicarbazone ligands showed no antiproliferative effect, but the corresponding platinum(II) and palladium(II) complexes inhibited cell proliferation and induced apoptosis. Platinum(II) complexes also displayed selective apoptotic activity in U937 cells but not in peripheral blood monocytes or the human hepatocellular carcinoma HepG2 cell line used to screen for potential hepatotoxicity. Present findings show that, in U937 cells, 1‐indanone thiosemicarbazones coordinated to palladium(II) were more cytotoxic than those complexed with platinum(II), although the latter were found to be more selective for leukemic cells suggesting that they are promising compounds with potential therapeutic application against hematological malignancies.
Aromatase and Dual Aromatase‐Steroid Sulfatase Inhibitors from the Letrozole and Vorozole Templates
Authors: Wood Paul M. , Woo L. W. Lawrence , Thomas Mark P. , Mahon Mary F. , Purohit Atul , Potter Barry V. L. 
Published: May 2011

AbstractConcurrent inhibition of aromatase and steroid sulfatase (STS) may provide a more effective treatment for hormone‐dependent breast cancer than monotherapy against individual enzymes, and several dual aromatase–sulfatase inhibitors (DASIs) have been reported. Three aromatase inhibitors with sub‐nanomolar potency, better than the benchmark agent letrozole, were designed. To further explore the DASI concept, a new series of letrozole‐derived sulfamates and a vorozole‐based sulfamate were designed and biologically evaluated in JEG‐3 cells to reveal structure–activity relationships. Amongst achiral and racemic compounds, 2‐bromo‐4‐(2‐(4‐cyanophenyl)‐2‐(1H‐1,2,4‐triazol‐1‐yl)ethyl)phenyl sulfamate is the most potent DASI (aromatase: IC50=0.87 nM; STS: IC50=593 nM). The enantiomers of the phenolic precursor to this compound were separated by chiral HPLC and their absolute configuration determined by X‐ray crystallography. Following conversion to their corresponding sulfamates, the S‐(+)‐enantiomer was found to inhibit aromatase and sulfatase most potently (aromatase: IC50=0.52 nM; STS: IC50=280 nM). The docking of each enantiomer and other ligands into the aromatase and sulfatase active sites was also investigated.
The Kinetics of Ferrocene Volatilisation from an Ionic Liquid
Authors: Fu Chaopeng , Aldous Leigh , Dickinson Edmund J. F. , Manan Ninie S. A. , Compton Richard G. 
Published: May 2011

AbstractThe volatilisation of ferrocene (Fc), dissolved in the ionic liquid N‐butyl‐N‐methylpyrrolidinium bis(trifluoromethylsulfonyl)imide, [C4mpyrr][NTf2], to the gas phase has been indirectly monitored by cyclic voltammetry and chronoamperometry. Simulation of the observed trends in concentration with time using a simple model allowed quantification of the process. Volatilisation of dissolved Fc under flowing wet and dry dinitrogen gas (N2) was found to be kinetically limited with a rate constant in the region of 2×10−7 cm s−1. The activation energy of diffusion for Fc was found to be 28.2±0.7 kJ mol−1, while the activation energy of volatilisation of Fc from [C4mpyrr][NTf2] to dry N2 was found to be 85±2 kJ mol−1.
Immobilization of Apoferritin‐Templated Seeds for Si Nanowire Growth
Authors: Zhang Zhang , Zhang Lianbing , Senz Stephan , Knez Mato 
Published: May 2011

AbstractSmall Au nanoparticles with a confined size are synthesized exclusively inside the protein cavity of apoferritin (Au‐apo). A modified immobilization process is used to decorate a large area of a Si substrate with monodisperse Au‐apo. After removing the protein, the immobilized Au nanoparticles act as catalysts for a subsequent sub‐10 nm diameter silicon nanowire (SiNW) growth by CVD. The immobilization of Au catalysts by linking the biotemplate to a Si substrate provides a possible route to control the location of the semiconductor NW in advance of the growth.
Parental disorders, childhood abuse, and binge eating in a large community sample
Authors: Ericsson Natalie Sachs‐ , Keel Pamela K. , Holland Lauren , Selby Edward A. , Verona Edelyn , Cougle Jesse R. , Palmer Emily 
Published: May 2011

AbstractObjective:Genetic and nonshared environmental factors are implicated in the etiology of binge eating behaviors (BEB), with genetic risk expressed as parental psychopathology. Traumatic experiences including child abuse predict onset of BEB. It is not clear if each separately contribute to BEB, or whether parental pathology leads to abuse which in turn influences BEB.Method:Data were obtained from the National Comorbidity Survey‐Replication (N = 2960). Through structural equation modeling, we estimated unique and combined effects of mother's and father's internalizing (INT) and externalizing (EXT) disorders, and child abuse on BEB.Results:Parental INT and EXT psychopathology contributed to child abuse and BEB. Abuse predicted BEB and partially mediated associations between parental psychopathology and BEB.Discussion:Results emphasize the value of models that incorporate nature and nurture to understand risk for psychopathology in offspring, with childhood abuse being one mediator of how parental psychopathology may reflect genetic risk and influence environmental risk. © 2011 by Wiley Periodicals, Inc. (Int J Eat Disord 2012;)
Batf3 transcription factor‐dependent DC subsets in murine CMV infection: Differential impact on T‐cell priming and memory inflation
Authors: Torti Nicole , Walton Senta M. , Murphy Kenneth M. , Oxenius Annette 
Published: May 2011

AbstractPriming of CD8+ T cells specific for viruses that interfere with the MHC class I presentation pathway is a challenge for the immune system and is believed to rely on cross‐presentation. Cytomegalovirus (CMV) infection induces vigorous CD8+ T‐cell responses despite its potent immune evasion strategies. Furthermore, CD8+ T cells specific for a subset of viral epitopes accumulate and are maintained at high levels exhibiting an activated phenotype – referred to as “inflationary T cells”. Taking advantage Batf3−/− mice in which the development of cross‐presenting CD8α+ and CD103+ DCs is severely compromised, we analyzed their role in the induction and inflation of murine (M)CMV‐specific CD8+ T‐cell responses. We found that priming of MCMV‐specific CD8+ T cells was severely impaired in the absence of cross‐presenting DCs. However, inflation of two immuno‐dominant MCMV‐specific CD8+ T‐cell populations was largely normal in the absence of cross‐presenting DCs, indicating that inflation during latency was mainly dependent on direct antigen presentation. These results highlight differential antigen presentation requirements during acute and latent MCMV infection.
Efficient generation of B lymphocytes by recognition of self‐antigens
Authors: Eschbach Cathrin , Bach Martina P. , Fidler Ingrid , Pelanda Roberta , Köhler Fabian , Rajewsky Klaus , Jumaa Hassan 
Published: May 2011

AbstractAntibody diversity is generated by a random gene recombination process with the inherent risk of the production of autoreactive specificities. The current view suggests that B cells expressing such specificities are negatively selected at an early developmental stage. Using the knock‐in model system of the 3‐83 autoreactive B‐cell antigen receptor (BCR) in combination with precursor‐BCR (pre‐BCR) deficiency, we show here that the 3‐83 BCR mediates efficient generation of B cells in the presence, but not the absence, of a strongly recognized auto‐antigen. Experiments with mixed bone marrow chimeras showed that combining the 3‐83 BCR with the corresponding auto‐antigen resulted in efficient reconstitution of B‐cell development in immune‐deficient mice. These results suggest that B cells are positively selected by recognition of self‐antigens during developmental stages that precede receptor editing. Moreover, the data indicate that the pre‐BCR functions as a specialized autoreactive BCR to initiate positive selection at a stage where the cells express immunoglobulin heavy but not light chains.
Electrochemical Behavior of a Glassy Carbon Electrode Chemically Modified with Nickel Pentacyanonitrosylferrate in Presence of Sulfur Compounds
Authors: Paim Leonardo L. , Hammer Peter , Stradiotto Nelson R. 
Published: May 2011

AbstractThe glassy carbon electrode was modified with a nickel pentacyanonitrosylferrate film by electrodeposition of Ni and subsequent derivatization with NaPCNF. The film was characterized by XPS and electrochemical methods. Cyclic voltammetry of the NiPCNF onto the GC shows a redox couple (FeIII/FeII) with E°′ of 538 mV (Ipa/Ipc around 1) and ΔEp of 93 mV in 0.5 mol L−1 KNO3, with a diffusion‐controlled process. There was a decrease of anodic peak currents of the film in the presence of sulfide and 2‐propanethiol due to a precipitation reaction on the film surface by nucleophilic attack.
“I still have a suitcase in Dresden …”
Authors: Unterstell Rembert 
Published: May 2011

AbstractAn opportunity to make new contacts in the international research community – following the 60th Nobel Laureate Meeting at Lake Constance, twenty young Indian researchers embarked on a tour of Germany',s research landscape, visiting universities and institutes around the country. We spoke with two of the participants about their experienceshttp://www.dfg.de/indien
Fukushima Daiichi: Implications for carbon-free energy, nuclear nonproliferation, and community resilience
Authors: Hall Howard L. 
Published: May 2011

Abstract
Implications of the nuclear power plant accidents at Fukushima Daiichi are explored in this commentary. In addition to questions of nuclear reactor regulatory standards, broader implications on noncarbon-emitting energy production, nuclear nonproliferation objectives, and community resilience and emergency response against catastrophic events are explored. Integr Environ Assess Manag 2011;7:406–408. © 2011 SETAC
Lessons in risk- versus resilience-based design and management
Authors: Park Jeryang , Seager Thomas P , Rao P Suresh C 
Published: May 2011

Abstract
The implications of recent catastrophic disasters, including the Fukushima Daiichi nuclear power plant accident, reach well beyond the immediate, direct environmental and human health risks. In a complex coupled system, disruptions from natural disasters and man-made accidents can quickly propagate through a complex chain of networks to cause unpredictable failures in other economic or social networks and other parts of the world. Recent disasters have revealed the inadequacy of a classical risk management approach. This study calls for a new resilience-based design and management paradigm that draws upon the ecological analogues of diversity and adaptation in response to low-probability and high-consequence disruptions. Integr Environ Assess Manag 2011;7:396–399. © 2011 SETAC
Environmental risk management for radiological accidents: Integrating risk assessment and decision analysis for remediation at different spatial scales
Authors: Yatsalo Boris , Sullivan Terrence , Didenko Vladimir , Linkov Igor 
Published: May 2011

Abstract
The consequences of the Tohuku earthquake and subsequent tsunami in March 2011 caused a loss of power at the Fukushima Daiichi nuclear power plant, in Japan, and led to the release of radioactive materials into the environment. Although the full extent of the contamination is not currently known, the highly complex nature of the environmental contamination (radionuclides in water, soil, and agricultural produce) typical of nuclear accidents requires a detailed geospatial analysis of information with the ability to extrapolate across different scales with applications to risk assessment models and decision making support. This article briefly summarizes the approach used to inform risk-based land management and remediation decision making after the Chernobyl, Soviet Ukraine, accident in 1986. Integr Environ Assess Manag 2011;7:393–395. © 2011 SETAC
A Bayesian network model for integrative river rehabilitation planning and management
Authors: Borsuk Mark E , Schweizer Steffen , Reichert Peter 
Published: May 2011

Abstract
As rehabilitation of previously channelized rivers becomes more common worldwide, flexible integrative modeling tools are needed to help predict the morphological, hydraulic, economic, and ecological consequences of the rehabilitation activities. Such predictions can provide the basis for planning and long-term management efforts that attempt to balance the diverse interests of river system stakeholders. We have previously reported on a variety of modeling methods and decision support concepts that can assist with various aspects of the river rehabilitation process. Here, we bring all of these tools together into a probability network model that links management actions, through morphological and hydraulic changes, to the ultimate ecological and economic consequences. Although our model uses a causal graph representation common to Bayesian networks, we do not limit ourselves to discrete-valued nodes or conditional Gaussian distributions as required by most Bayesian network implementations. This precludes us from carrying out easy probabilistic inference but gives us the advantages of functional and distributional flexibility and enhanced predictive accuracy, which we believe to be more important in most environmental management applications. We exemplify model application to a large, recently completed rehabilitation project in Switzerland. Integr Environ Assess Manag 2012; 8: 462–472. © SETAC
Soil vulnerability for cesium transfer
Authors: Vandenhove Hildegarde , Sweeck Lieve 
Published: May 2011

Abstract
The recent events at the Fukushima Daiichi nuclear power plant in Japan have raised questions about the accumulation of radionuclides in soils and the possible impacts on agriculture surrounding nuclear power plants. This article summarizes the knowledge gained after the nuclear power plant accident in Chernobyl, Ukraine, on how soil parameters influence soil vulnerability for radiocesium bioavailability, discusses some potential agrochemical countermeasures, and presents some predictions of radiocesium crop concentrations for areas affected by the Fukushima accident. Integr Environ Assess Manag 2011;7:374–378. © 2011 SETAC
Effects of ionizing radiation on wildlife: What knowledge have we gained between the Chernobyl and Fukushima accidents?
Authors: Beresford Nicholas A , Copplestone David 
Published: May 2011

Abstract
The recent events at the Fukushima Daiichi nuclear power plant in Japan have raised questions over the effects of radiation in the environment. This article considers what we have learned about the radiological consequences for the environment from the Chernobyl accident, Ukraine, in April 1986. The literature offers mixed opinions of the long-term impacts on wildlife close to the Chernobyl plant, with some articles reporting significant effects at very low dose rates (below natural background dose rate levels in, for example, the United Kingdom). The lack of agreement highlights the need for further research to establish whether current radiological protection criteria for wildlife are adequate (and to determine if there are any implications for human radiological protection). Integr Environ Assess Manag 2011;7:371–373. © 2011 SETAC
Impaired tumor rejection by memory CD8 T cells in mice with NKG2D dysfunction
Authors: André Maya Caroline , Sigurdardottir Dagmar , Kuttruff Sabrina , Pömmerl Beate , Handgretinger Rupert , Rammensee Hans‐Georg , Steinle Alexander 
Published: May 2011

AbstractCytotoxic T cells are important effectors for robust antitumor immune responses. However, tumor‐infiltrating CD8 T cells are often functionally impaired. Insufficient antitumor activity of CD8 T cells can be due to a lack of costimulatory signals. NKG2D is such a costimulatory receptor on CD8 T cells that facilitates immunorecognition of stressed and malignant cells, promotes tumor rejection by NK and CD8 T cells and contributes to immunosurveillance of spontaneous malignancies. Previous reports suggested an involvement of NKG2D in establishing CD8 T cell‐mediated antitumor memory. However, the significance of NKG2D for the generation and effector phase of memory CD8 T cell responses is largely unknown. To address these issues, we made use of a transgenic mouse model (H2‐Kb‐MICA mice) where the human NKG2D ligand MICA is ubiquitously and constitutively expressed resulting in a severe dysfunction of NKG2D. Both, ovalbumin (OVA)‐specific (H2‐Kb/OVA257–264) memory CD8 T cells arisen from the endogenous T cell pool and adoptively transferred OVA‐specific OT‐I memory cells were unable to control growth of an OVA‐expressing lymphoma in H2‐Kb‐MICA mice. While expansion of memory T cells in these mice on antigen challenge was not different from controls, CD8 memory T cells of H2‐Kb‐MICA mice did not effectively eliminate tumor cells in vivo. Altogether, our data suggest that NKG2D has no major role in the generation and expansion of memory CD8 T cells, but rather substantially enhances the cytolytic effector responses of reactivated memory T cells and thereby contributes to an efficacious tumor rejection.
Switch from cytology‐based to human papillomavirus test‐based cervical screening: Implications for colposcopy
Authors: Porras Carolina , Wentzensen Nicolas , Rodríguez Ana C. , Morales Jorge , Burk Robert D. , Alfaro Mario , Hutchinson Martha , Herrero Rolando , Hildesheim Allan , Sherman Mark E. , Wacholder Sholom , Solomon Diane , Schiffman Mark 
Published: May 2011

AbstractHuman papillomavirus (HPV) testing is more sensitive than cytology; some cervical cancer prevention programs will switch from cytology to carcinogenic HPV test‐based screening. The objective of our study is to evaluate the clinical implications of a switch to HPV test‐based screening on performance and workload of colposcopy. Women in the population‐based, 7‐year Guanacaste cohort study were screened at enrollment using cytology. We also took another specimen for HPV DNA testing and collected magnified cervical photographic images (cervigrams). A final case diagnosis (≥cervical intraepithelial neoplasia [CIN] grade 3, CIN2, <CIN2) was assigned at exit. Using the cervigram as a surrogate of colposcopy impression, we evaluated the impact of changing screening method from cytology to carcinogenic HPV testing on the distribution of enrollment colposcopic impression and on the predictive values of positive and negative colposcopic impressions for the cumulative 7‐year detection of ≥CIN2 and ≥CIN3. A program based on immediate colposcopic referral after positive HPV would immediately identify as high risk more of the cumulative ≥CIN2 cases than conventional cytology, because of an increased number of referrals. However, the proportion of women that would have visible lesions at referral to colposcopy and the sensitivity versus specificity trade‐off of the colposcopic impressions would be similar to programs using cytology (≥ atypical squamous cells of unknown significance [ASCUS]) for referral. The major concern with switching from cytology to more sensitive HPV screening is management of the many HPV‐positive women, including those with still nonvisible ≥CIN2 lesions. Our data support the need for a nonvisual diagnostic method to guide management and treatment of HPV‐positive women.