bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antitumor effects of deracoxib treatment in 26 dogs with transitional cell carcinoma of the urinary bladder
Authors: McMillan Sarah K. , Boria Pedro , Moore George E. , Widmer William R. , Bonney Patty L. , Knapp Deborah W. 
Published: October 2011

Abstract
Objective—To evaluate the antitumor activity and toxic effects of deracoxib, a selective cyclooxygenase-2 inhibitor, in dogs with transitional cell carcinoma (TCC) of the urinary bladder.
Design—Clinical trial.
Animals—26 client-owned dogs with naturally occurring, histologically confirmed, measurableTCC of the urinary bladder.
Procedures—Dogs were treated PO with deracoxib at a dosage of 3 mg/kg/d (1.36 mg/lb/d) as a single-agent treatment for TCC. Tumor response was assessed via radiography, abdominal ultrasonography, and ultrasonographic mapping of urinary bladder masses. Toxic effects of deracoxib administration in dogs were assessed through clinical observations and hematologic and biochemical analyses.
Results—Of 24 dogs for which tumor response was assessed, 4 (17%) had partial remission, 17 (71%) had stable disease, and 3 (13%) had progressive disease; initial response could not be assessed in 2 of 26 dogs. The median survival time was 323 days. Median time to progressive disease was 133 days. Renal, hepatic, and gastrointestinal abnormalities attributed to deracoxib administration were noted in 4% (1/26), 4% (1/26), and 19% (5/26) of dogs, respectively.
Conclusions and Clinical Relevance—Results indicated that deracoxib was generally well tolerated by dogs and had antitumor activity against TCC.
Comparison of early socialization practices used for litters of small-scale registered dog breeders and nonregistered dog breeders
Authors: Korbelik Juraj , Rand Jacquie S. , Morton John M. 
Published: October 2011

Abstract
Objective—To compare early socialization practices between litters of breeders registered with the Canine Control Council (CCC) and litters of nonregistered breeders advertising puppies for sale in a local newspaper.
Design—Retrospective cohort study.
Animals—80 litters of purebred and mixed-breed dogs from registered (n = 40) and non-registered (40) breeders.
Procedures—Registered breeders were randomly selected from the CCC website, and nonregistered breeders were randomly selected from a weekly advertising newspaper. The litter sold most recently by each breeder was then enrolled in the study. Information pertaining to socialization practices for each litter was obtained through a questionnaire administered over the telephone.
Results—Registered breeders generally had more breeding bitches and had more litters than did nonregistered breeders. Litters of registered breeders were more likely to have been socialized with adult dogs, people of different appearances, and various environmental stimuli, compared with litters of nonregistered breeders. Litters from registered breeders were also much less likely to have been the result of an unplanned pregnancy.
Conclusions and Clinical Relevance—Among those breeders represented, litters of registered breeders received more socialization experience, compared with litters of nonregistered breeders. People purchasing puppies from nonregistered breeders should focus on socializing their puppies between the time of purchase and 14 weeks of age. Additional research is required to determine whether puppies from nonregistered breeders are at increased risk of behavioral problems and are therefore more likely to be relinquished to animal shelters or euthanized, relative to puppies from registered breeders.
Penetrating injuries to the frog (cuneus ungulae) and collateral sulci of the foot in equids: 63 cases (1998–2008)
Authors: Kilcoyne Isabelle , Dechant Julie E. , Kass Philip H. , Spier Sharon J. 
Published: October 2011

Abstract
Objective—To determine the outcome of penetrating injuries to the central region of the foot in equids and identify factors that may affect treatment and outcome.
Design—Retrospective case series.
Animals—63 equids (61 horses, 1 pony, and 1 mule).
Procedures—Records of equids incurring puncture wounds through the frog (cuneus ungulae) or collateral sulci of the foot between 1998 and 2008 were reviewed. Evaluated factors that were hypothesized to affect outcome included signalment, degree of lameness, foot affected, duration between injury and admission, and treatment. Injuries were graded from 1 (< 1 inch; involving superficial corium only) to 4 (involving a synovial structure) on the basis of severity of penetration as determined by radiographic evidence or findings on synoviocentesis at the time of admission.
Results—Overall, 60% (38/63) of equids returned to soundness. Thirteen equids were euthanized on the basis of synovial structure involvement and financial constraints. Of 35 equids that were treated conservatively, which may have included undergoing a surgical procedure with the horse standing, 32 (91.4%) returned to their previous level of soundness. Fifteen equids underwent surgical treatment under general anesthesia, of which 6 (40%) became sound for intended use. Ten of 34 (29%) equids with synovial structure involvement regained soundness. Equids treated earlier after injury had a better prognosis. Equids with a hind foot injury had a more favorable outcome than those with a forefoot injury.
Conclusions and Clinical Relevance—Results suggested that penetrating injuries located centrally in the foot of equids without involvement of a synovial structure have a favorable prognosis, especially if managed early. Penetration of a synovial structure provided a poor prognosis.
Gastric and enteric phytobezoars caused by ingestion of persimmon in equids
Authors: Banse Heidi E. , Gilliam Lyndi L. , House Amanda M. , McKenzie Harold C. , Johnson Philip J. , Lopes Marco A. F. , Carmichael Robert J. , Groover Erin S. , LaCarrubba Alison M. , Breshears Melanie A. , Brosnahan Margaret M. , Funk Rebecca , Holbrook Todd C. 
Published: October 2011

Abstract
Case Description—13 equids (10 horses, 2 donkeys, and 1 pony) were examined for signs of colic (n = 7), weight loss (6), anorexia (3), and diarrhea (2). Ten equids were evaluated in the fall (September to November). Seven equids had a history of persimmon ingestion.
Clinical Findings—A diagnosis of phytobezoar caused by persimmon ingestion was made for all equids. Eight equids had gastric persimmon phytobezoars; 5 had enteric persimmon phytobezoars. Gastroscopy or gastroduodenoscopy revealed evidence of persimmon ingestion in 8 of 10 equids in which these procedures were performed.
Treatment and Outcome—2 of 13 equids were euthanatized prior to treatment. Supportive care was instituted in 11 of 13 equids, including IV administration of fluids (n = 8) and treatment with antimicrobials (5), NSAIDs (5), and gastric acid suppressants (4). Persimmon phytobezoar–specific treatments included dietary modification to a pelleted feed (n = 8); oral or nasogastric administration of cola or diet cola (4), cellulase (2), or mineral oil (2); surgery (4); and intrapersimmon phytobezoar injections with acetylcysteine (1). Medical treatment in 5 of 7 equids resulted in resolution of gastric persimmon phytobezoars. Seven of 8 equids with gastric persimmon phytobezoars and 1 of 5 equids with enteric persimmon phytobezoars survived > 1 year after hospital discharge.
Clinical Relevance—Historical knowledge of persimmon ingestion in equids with gastrointestinal disease warrants gastroduodenoscopy for evaluation of the presence of persimmon phytobezoars. In equids with gastric persimmon phytobezoars, medical management (including administration of cola or diet cola and dietary modification to a pelleted feed) may allow for persimmon phytobezoar dissolution.
Comparison of Optical Aberrations and Contrast Sensitivity Between Aspheric and Spherical Intraocular Lenses
Authors: Morales Eduardo L. , Rocha Karolinne M. , Chalita Maria Regina , Nosé Walton , Avila Marcos P. 
Published: October 2011

PURPOSE:
To compare visual acuity, aberrometry, and contrast sensitivity in patients who had a spherical intraocular lens (IOL) (SoFlex SE, Bausch & Lomb) implanted in one eye and an aspheric IOL (SofPort AO, Bausch & Lomb) implanted in the fellow eye during uncomplicated cataract surgery.

METHODS:

A prospective, multicenter, double-blind study was performed. Forty patients (80 eyes) underwent bilateral phacoemulsification with implantation of a spherical IOL in one eye and an aspheric IOL in the fellow eye. Postoperatively, visual acuity, aberrometry, and contrast sensitivity tests were performed. Statistical analyses were performed using Student
t
and Wilcoxon tests, and mixed effects were used for each contrast condition situation.

RESULTS:
Thirty-nine patients (30 women, 9 men; 78 eyes) with a mean age of 69.3±6.17 years (range: 51 to 82 years) completed the study. No statistical differences were found regarding visual acuity among eyes. Lower levels of higher order aberrations were achieved in the aspheric group. No statistical difference between groups under photopic conditions was noted. In low spatial frequencies, better performance was observed with the aspheric IOL under mesopic conditions. In high spatial frequencies, the spherical IOL produced better quality of vision. Comparing mesopic conditions with glare, visual performance was statistically better using the aspheric IOL.

CONCLUSIONS:
Cataract surgery results cannot be measured by means of visual acuity alone. Quality of vision must be considered, and implantation of IOLs with low levels of spherical aberrations and better contrast sensitivity are preferred. In this study, the aspheric IOL demonstrated better visual function, especially at night, when compared with spherical IOLs.
Analysis of Microkeratome Thin Flap Architecture Using Fourier-domain Optical Coherence Tomography
Authors: Rocha Karolinne Maia , Randleman J. Bradley , Stulting R. Doyle 
Published: October 2011

PURPOSE:
To assess the corneal architecture and reproducibility of LASIK flap thickness created by the Amadeus II mechanical microkeratome (Ziemer Ophthalmic Systems AG) using Fourier-domain optical coherence tomography (OCT; Optovue Inc).

METHODS:
Anterior segment Fourier-domain OCT was used to analyze the morphology of 58 LASIK flaps from 30 patients created with the Amadeus II microkeratome 140-μm head and ML7090CLB blades (Med-Logics Inc) at 2 weeks postoperatively. Flap thickness was assessed at 10 points across the central 6 mm of the cornea (horizontal and vertical meridians). Postoperative central corneal flap thickness measured by Fourier-domain OCT was compared with intraoperative ultrasound pachymetry measurements.

RESULTS:

No significant difference was noted between central flap thickness measured by intraoperative pachymetry (107.2±14 μm) and postoperative OCT (111.7±11 μm;
P
=.07, correlation coefficient=0.86). Fourier-domain OCT measurements demonstrated functionally planar flap architecture (standard deviation [SD] of thickness across the flap=4.9 μm, SD range across the flap=2 to 9 μm) for the microkeratome flaps.

CONCLUSIONS:
The Amadeus II microkeratome with Med-Logics blades created thin, reproducible, functionally planar flaps as measured by Fourier-domain OCT. Central flap thickness measured by intraoperative ultrasound pachymetry was equivalent to that measured 2 weeks postoperatively by OCT.
Internal Aberrations and Optical Quality After Femtosecond Laser Anterior Capsulotomy in Cataract Surgery
Authors: Miháltz Kata , Knorz Michael C. , Alió Jorge L. , Takács Ágnes I. , Kránitz Kinga , Kovács Illés , Nagy Zoltán Z. 
Published: October 2011

PURPOSE:
To compare ocular and internal aberrations after femtosecond laser anterior capsulotomy and continuous curvilinear capsulorrhexis in cataract surgery.

METHODS:
In this prospective study, anterior capsulotomy was performed during cataract surgery with an intraocular femtosecond (FS) laser (Alcon LenSx Inc) in 48 eyes. As a control group, continuous curvilinear capsulorrhexis (CCC) was performed in 51 eyes. Wavefront aberrometry, corneal topography, and objective visual quality were measured using the OPD-Scan (NIDEK Co Ltd). Vertical and horizontal tilt, coma, and visual quality metrics were evaluated separately to determine whether the source of aberrations was ocular or internal. Main outcome measures included postoperative residual refraction, uncorrected and corrected visual acuities, ocular and internal aberrations, Strehl ratio, and modulation transfer function (MTF).

RESULTS:
No statistically significant differences were noted between the FS and CCC groups, respectively, in postoperative sphere (−0.60±1.50 vs −0.50±1.40 diopters [D]), postoperative cylinder (1.30±1.01 vs 1.10±1.10 D), uncorrected distance visual acuity (0.86±0.15 vs 0.88±0.08), or corrected distance visual acuity (0.97±0.08 vs 0.97±0.06). The FS group had significantly lower values of intraocular vertical tilt (−0.05±0.36 vs 0.27±0.57) and coma (−0.003±0.11 vs 0.1±0.15), and significantly higher Strehl ratios (0.02±0.02 vs 0.01±0.01) and MTF values at all measured cycles per degree, compared to the CCC group.

CONCLUSIONS:
Capsulotomy performed with an intraocular FS laser induced significantly less internal aberrations measured by the NIDEK OPD-Scan aberrometer compared to eyes that underwent CCC, which may result in better optical quality after the procedure.
Visual Outcomes After Epi-LASIK and PRK for Low and Moderate Myopia
Authors: Sia Rose K. , Coe Charles D. , Edwards Jayson D. , Ryan Denise S. , Bower Kraig S. 
Published: October 2011

PURPOSE:
To evaluate visual outcomes following epi-LASIK compared to photorefractive keratectomy (PRK).

METHODS:
Of a total 294 patients aged ≥21 years, 145 (290 eyes) underwent epi-LASIK and 149 (298 eyes) underwent PRK for low to moderate myopia or myopic astigmatism. Epi-LASIK was performed with the Amadeus II epikeratome (Abbott Medical Optics) and PRK with the Amoils rotary epithelial brush (Innovative Excimer Solutions). All ablations were performed using the same excimer laser system. Outcome measures included intraoperative complications, corneal reepithelialization, postoperative pain, uncorrected distance visual acuity (UDVA), manifest refraction spherical equivalent (MRSE), corrected distance visual acuity (CDVA), corneal haze, and quality of vision.

RESULTS:

Mean preoperative MRSE was −2.97±1.19 diopters (D) for epi-LASIK versus −2.95±1.06 D for PRK. Complete reepithelialization was achieved by postoperative day 4 in 46.9% of epi-LASIK eyes versus 92.4% of PRK eyes, with superior UDVA at postoperative day 1 in the PRK group (
P
=.002). Using Fisher exact test, a significantly higher percentage of epi-LASIK eyes compared to PRK eyes achieved 20/15 or better at 1 month (25.8% vs 17.8%,
P
=.031), 3 months (62.3% vs 49.3%,
P
=.004), 6 months (77.1% vs 57.9%,
P
<.001), and 12 months (75.9% vs 61.9%,
P
=.002). A change in MRSE >0.50 D occurred in 8.4% of epi-LASIK eyes within the 3- and 12-month interval versus 17.7% of PRK eyes (
P
=.04). No differences were noted between the two procedures in CDVA or clinically significant haze.

CONCLUSIONS:
Epi-LASIK showed superior refractive efficacy and stability but required more time for wound healing, resulting in inferior early visual outcomes and a tendency to overcorrect higher refractive errors compared to PRK. Both treatments were safe and comparable in terms of pain and haze formation.
Introduction and Historical Overview of DNA Sequencing
Authors: Kenneth Nelson F. , Snyder Michael , Gardner Andrew F. , Hendrickson Cynthia L. , Shendure Jay A. , Porreca Gregory J. , Church George M. , Ausubel Frederick M. , Ju Jingyue , Kieleczawa Jan , Slatko Barton E. 
Published: October 2011

AbstractThe process of DNA sequencing has made tremendous strides in throughput, improved accuracy, ease of production, and lowered cost. As the practice of DNA sequencing has improved, so has the downstream data analysis with sophisticated databases and bioinformatics tools. Together, these advances have enlarged the number of applications upon which DNA sequencing can be brought to bear. This introductory unit provides a description of DNA sequencing with a focus on current and “NextGen” (second and third generation) automated technologies and applications. Curr. Protoc. Mol. Biol. 96:7.0.1‐7.0.18. © 2011 by John Wiley & Sons, Inc.
Overview of DNA Sequencing Strategies
Authors: Shendure Jay A. , Porreca Gregory J. , Church George M. , Gardner Andrew F. , Hendrickson Cynthia L. , Kieleczawa Jan , Slatko Barton E. 
Published: October 2011

AbstractEfficient and cost‐effective DNA sequencing technologies are critical to the progress of molecular biology. This overview of DNA sequencing strategies provides a high‐level review of seven distinct approaches to DNA sequencing: (a) dideoxy sequencing; (b) solid phase sequencing; (c) sequencing‐by‐hybridization; (d) mass spectrometry; (e) cyclic array sequencing; (f) microelectrophoresis; and (g) nanopore sequencing. Other platforms currently in development are also briefly described. The primary focus here is on Sanger dideoxy sequencing, which has been the dominant technology since 1977, and on cyclic array strategies, for which several competitive implementations have been developed since 2005. Because the field of DNA sequencing is changing rapidly, this unit represents a snapshot as of September, 2011. Curr. Protoc. Mol. Biol. 96:7.1.1‐7.1.23. © 2011 by John Wiley & Sons, Inc.
High‐Throughput Sequencing of PCR Products Tagged with Universal Primers Using 454 Life Sciences Systems
Authors: Daigle Derek , Simen Birgitte B. , Pochart Pascale 
Published: October 2011

AbstractThis unit describes a method to convert PCR products (amplicons) flanked by universal M13 primers into a library for use on all 454 Sequencing Systems (454 Life Sciences, a Roche Company). This is especially useful for simultaneous sequencing and analysis of large numbers of amplicons or for the detection of minor variations within the amplified products. The method described here involves preparing a library of DNA with specific primers containing adaptor sequences recognized by the GS Junior System sequencing process. The data from the sequencing run are processed and analyzed by 454 Life Sciences software. This approach allows for multiplexing of a large number of amplicons to streamline processing and analysis. Any pre‐existing universally tagged amplicon, primer set, or plasmid with M13 sequences flanking the cloning site can be used in this protocol. Curr. Protoc. Mol. Biol. 96:7.5.1‐7.5.14. © 2011 by John Wiley & Sons, Inc.
Influence of Background Speech and Music in Interrupted Reading: An Eye‐Tracking Study
Authors: Cauchard Fabrice , Cane James E. , Weger Ulrich W. 
Published: October 2011

SummaryThe current study examined the influence of interruption, background speech and music on reading, using an eye movement paradigm. Participants either read paragraphs while being exposed to background speech or music or read the texts in silence. On half of the trials, participants were interrupted by a 60‐second audio story before resuming reading the paragraph. Interruptions increased overall reading time, but the reading of text following the interruption was quicker compared with baseline. Background speech and music did not modulate the interruption effects, but the background speech slowed down the reading rate compared with reading in the presence of music or reading in silence. The increase in reading time was primarily due to an increase in the time spent rereading previously read words. We argue that the observed interruption effects are in line with a theory of long‐term working memory, and we present practical implications for the reported background speech effects. Copyright © 2011 John Wiley & Sons, Ltd.
Type I Collagen Immobilized Poly(caprolactone) Nanofibers: Characterization of Surface Modification and Growth of Fibroblasts
Authors: Krithica Natarajan , Natarajan Venkatachalam , Madhan Balaraman , Sehgal Praveen Kumar , Mandal Asit Baran 
Published: October 2011

AbstractPoly(caprolactone) (PCL) electrospun nanofibers were modified by aminolysis and collagen was immobilized on the aminolysed PCL nanofibers. Considering low immunogenic response collagen elicits, immobilization of the same is anticipated to enhance the tissue engineering application of the PCL nanofibers. Amino groups were introduced into PCL nanofibers through aminolysis process. Aminolysis of PCL nanofibers was confirmed by electron dispersive X‐ray analysis (EDX). Collagen was immobilized on aminolysed PCL nanofibers using glutaraldehyde as crosslinker. The collagen crosslinking on to PCL nanofibers was established by attenuated total reflectance‐Fourier transform infrared (ATR‐FTIR) spectroscopy. The fiber morphologies of PCL nanofibers at different stages were characterized by scanning electron microscopy (SEM). The change in hydrophobicity of PCL nanofibers due to aminolysis and collagen immobilization was determined by water contact angle measurements. Aminolysis followed by collagen immobilization had reduced the intrinsic hydrophobicity of PCL nanofibers. NIH 3T3 fibroblasts were cultured for 2 days on PCL nanofibers, aminolysed PCL nanofibers, and aminolysed PCL nanofibers crosslinked with collagen. Cell attachment and growth were observed by MTT assay in each case. Collagen immobilization improved the biocompatibility of the PCL nanofibers. Thus the modified PCL nanofibers can be used as suitable broad spectrum scaffold for skin, cartilage, bone, cardiac constructs for efficient tissue engineering applications.
Nanocrystalline Titania‐Supported Palladium(0) Nanoparticles for Suzuki–Miyaura Cross‐Coupling of Aryl and Heteroaryl Halides
Authors: Sreedhar B. , Yada Divya , Reddy P. Surendra 
Published: October 2011

AbstractThe Suzuki cross‐coupling reaction of various aryl and heteroaryl halides with arylboronic and heteroarylboronic acids was studied using a titania‐supported palladium(0) catalyst at room temperature under air. The conversion and selectivity results obtained for many substrates were excellent and similar to those provided by more active or even homogeneous catalysts. The methodology is similarly effective using 2‐bromo‐3,4,5‐trimethoxybenzaldehyde as the coupling partner and gave products in good yield. Furthermore, it has been shown that it is useful for the synthesis of terphenyl and tetraphenyls. The catalyst is quantitatively recovered from the reaction by simple filtration and reused for a number of cycles without significant loss of activity. Inductively coupled plasma (ICP) mass‐spectrometric analysis of the filtrate from the reaction mixture demonstrated that the palladium metal hardly leached into the solution within the limits of the detector (1 ppm), thus suggesting that the present Suzuki–Miyaura reaction proceeded by heterogeneous catalysis.
Bismuth(III) Triflate‐Based Catalytic Direct Opening of Oleanolic Hydroxy‐γ‐lactones to Afford 12‐Oxo‐28‐carboxylic Acids
Authors: Salvador Jorge A. R. , Moreira Vânia M. , Pinto Rui M. A. , Leal Ana S. , Le Roux Christophe 
Published: October 2011

AbstractThe bismuth(III) triflate‐based catalytic direct opening of oleanolic hydroxy‐γ‐lactones affords the corresponding 12‐oxo‐28‐carboxylic acid derivatives, in both acetonitrile and dichloromethane, at reflux, in high yields. Participation of an in situ generated Brønsted acid species from bismuth(III) triflate is most likely involved in the reaction mechanism. Full structural elucidation of the products obtained has been performed by 1D and 2D NMR techniques.