bluuebunny/crossref_metadata_2025_split huggingface dataset:
Resilient liner vs. clip attachment effect on peri‐implant tissues of bar‐implant‐retained mandibular overdenture: a 1‐year clinical and radiographical study
Authors: Elsyad Moustafa Abdou , Shoukouki Ali Hamed EL 
Published: March 2010

AbstractPurpose: The aim of this study was to compare between the effects of resilient liner and clip attachments of bar‐implant‐retained mandibular overdenture on peri‐implant tissues.Materials and methods: In a randomized‐controlled clinical trial, 30 edentulous male patients (mean age 62.5 years) were equally assigned to two groups. In each patient, two implants were inserted in the canine area of the mandible using a two‐stage surgical protocol. After 3 months, the implants were connected with resilient bars. Mandibular overdentures were retained to the bars with either clips (group I) or silicone‐resilient liners (group II). Peri‐implant tissues were evaluated clinically (with regard to plaque scores, gingival scores and probing depths) and radiographically (with regard to peri‐implant vertical and horizontal alveolar bone changes). Evaluations were performed at the time of overdenture insertion (T0), 6 months (T6) and 12 months (T12) after overdenture insertion.Results: After 12 months of using bar‐implant‐retained mandibular overdenture, the resilient liner attachment had significantly decreased peri‐implant plaque score, gingival score, probing depth, vertical and horizontal bone loss when compared with the clip attachment.Conclusion: Within the limitations of this study, and in terms of peri‐implant tissue health of bar‐implant‐retained mandibular overdenture, we recommend resilient liner rather than clip attachment. To cite this article:
Elsyad MA, EL Shoukouki AH. Resilient liner vs. clip attachment effect on peri‐implant tissues of bar‐implant‐retained mandibular overdenture: a 1‐year clinical and radiographical study. Clin. Oral Impl. Res. 21, 2010; 473–480 doi: 10.1111/j.1600‐0501.2009.01879.x
Relationship between cortical bone thickness or computerized tomography‐derived bone density values and implant stability
Authors: Merheb Joe , Van Assche Nele , Coucke Wim , Jacobs Reinhilde , Naert Ignace , Quirynen Marc 
Published: March 2010

AbstractAims: To explore the relationship between primary implant stability and different parameters related to implant or bone properties.Materials and methods: Twenty‐four patients received a total of 136 Straumann SLActive implants. Resonance frequency analysis (RFA) was performed at implant placement, and RFA and Periotest (PTV) were scored at loading. Bone density [Hounsfield (HU) scores] and coronal cortical thickness at osteotomy sites were measured from pre‐operative computerized tomography scans.Results: Implant length, diameter or the presence of bony dehiscence did not have a significant effect on the mean RFA scores at implant insertion. Significant linear relations were found between RFA or PTV scores and HU values (P<0.05), or cortical bone thickness (P<0.05), both at insertion as well as at loading.Conclusion: RFA and PTV scores can be predicted based on implant and especially bone related factors. To cite this article:
Merheb J, Van Assche N, Coucke W, Jacobs R, Naert I, Quirynen M. Relationship between cortical bone thickness or computerized tomography‐derived bone density values and implant stability. Clin. Oral Impl. Res. 21, 2010; 612–617. doi: 10.1111/j.1600‐0501.2009.01880.x
The effect of deproteinized bovine bone on osteoblast growth factors and proinflammatory cytokine production
Authors: Amerio Paolo , Vianale Giovina , Reale Marcella , Muraro Raffaella , Tulli Antonello , Piattelli Adriano 
Published: March 2010

AbstractObjective: To test the ability of Bio‐Oss® in inducing growth factors and proinflammatory cytokines that may have a role in inflammation after grafting, bone resorption, remodeling and in the homeostasis of osteoblasts.Material and methods: Normal human osteoblasts were seeded in Petri dishes containing granules of Bio‐Oss®, cells were harvested after confluency and RNA was extracted. Reverse transcriptase polymerase chain reaction was performed using specific primers for osteonectin, bone sialoprotein (BSP), bone morphogenetic protein (BMP)‐2 and BMP‐7, platelet‐derived growth factor (PDGF), interleukin 6 (IL‐6), tumor necrosis factor α (TNF‐α) and integrin β1. Glycerol‐3‐phosphate dehydrogenase was used as the housekeeping gene and normal human osteoblasts grown on Petri dishes without Bio‐Oss® granules were used as negative controls.Results: Osteoblast grown on Bio‐Oss® showed a normal RNA expression of osteonectin, integrin β1 and PDGF. However, compared with control osteoblasts it showed a reduced expression of BSP, BMP‐2 and BMP‐7, IL‐6 and TNF‐α.Conclusions: Our findings further support the evidence that Bio‐Oss® is an excellent biomaterial that does not enhance the production of proinflammatory cytokines. To cite this article:
Amerio P, Vianale G, Reale M, Muraro R, Tulli A, Piattelli A. The effect of deproteinized bovine bone on osteoblast growth factors and proinflammatory cytokine production. Clin. Oral Impl. Res. 21, 2010; 650–655. doi: 10.1111/j.1600‐0501.2009.01881.x
Combined chelation therapy in thalassemia major with deferiprone and desferrioxamine: a retrospective study
Authors: Ricchi Paolo , Ammirabile Massimiliano , Spasiano Anna , Costantini Silvia , Cinque Patrizia , Di Matola Tiziana , Pagano Leonilde , Prossomariti Luciano 
Published: March 2010

Abstract Objectives: The benefits of combined chelation therapy with daily deferiprone (DFP) and subcutaneous desferrioxamine (DFO) have been widely reported in literature. We retrospectively evaluated the efficacy of different schedules of combined chelation therapy and the incidence of adverse events. Methods: We evaluated 36 patients affected by thalassemia major treated with combined chelation therapy. Patients were subdivided into four treatment arms according to severity of iron overload and previous onset of adverse events to DFP therapy: Group 1 (13 pts) DFP 75 mg/kg per d plus DFO (25–35 mg/kg per d for 5 d); Group 2 (6 pts) DFP 50 mg/kg per d plus DFO (25–35 mg/kg for 5 d), Group 3 (10 pts) DFP 75 mg/kg per d plus DFO (25–35 mg/kg for 3 d), and Group 4 (7 pts) DFP 50 mg/kg per d plus DFO (25–35 mg/kg for 3 d). Change in serum ferritin level was evaluated in all patients. Results: Overall, ferritin decreased from 2592 ± 1701 to 899 ± 833 ng/mL (P < 0.001). All treatments were able to reduce ferritin levels, but in patients of group 1 and group 2 the highest mean decrease in serum ferritin level and the greatest improvement in liver iron concentration (LIC) and in T2* values were observed. Conclusions: This study showed that the administration of DFO for 5 d a wk in combination with daily administration of DFP at 75 mg/Kg seemed to be the most efficacy and rapid method for reducing iron overload at liver and heart level. Furthermore, the use of different schedules of combined DFO and DFP administration was not associated with different incidence of adverse effects between the groups.
Discovery of a patient with strongly suspected bullous pemphigoid in a ward by oral health care providers
Authors: Kanda N , Soga Y , Meguro M , Tanabe A , Yagi Y , Himuro Y , Fujiwara Y , Takashiba S , Kobayashi N 
Published: March 2010

To cite this article:

Int J Dent Hygiene
9, 2011; 159–162 DOI: 10.1111/j.1601‐5037.2010.00448.x Kanda N, Soga Y, Meguro M, Tanabe A, Yagi Y, Himuro Y, Fujiwara Y, Takashiba S, Kobayashi N. Discovery of a patient with strongly suspected bullous pemphigoid in a ward by oral health care providers.Abstract:  Objectives:  Oral health care providers may discover systemic diseases incidentally from signs observed in the oral cavity. Here, we report a case in which oral health care providers in a hospital discovered a patient with strongly suspected bullous pemphigoid (BP), which is a relatively rare but important disease, in a ward.Methods:  The patient was a 78‐year‐old Japanese woman admitted to our hospital because of severe Alzheimer’s disease. We discovered recurrent ulcers in the oral mucosa and skin when performing oral care in her ward. Biopsy could not be performed safely because of involuntary biting. We performed blood tests for anti‐BP180‐NC16a antibody, which is autoantibody specific for BP.Results:  The patient had a very high anti‐BP180‐NC16a antibody titre. We consulted a dermatologist regarding her clinical course and the clinical features of the oral mucosa and skin along with blood test results. BP was very strongly suspected.Discussion:  In cases in which oral health care providers suspect their patients may have BP, appropriate examination and provision of information to the doctor are important. Oral health care providers should have knowledge about systemic diseases, the signs of which appear in oral cavity to avoid missing important systemic diseases.
Is the combination of topsoil replacement and inoculation with plant material an effective tool for the restoration of threatened sandy grassland?
Authors: Eichberg Carsten , Storm Christian , Stroh Michael , Schwabe Angelika 
Published: March 2010

AbstractQuestion: Is it possible to restore dry calcareous inland sand ecosystems with their characteristic plant community structure within a 4‐yr period by means of combined abiotic–biotic techniques (topsoil replacement, inoculation with raked/mown plant material from target areas)?Location: Upper Rhine valley, Germany.Methods: Two 4‐year experiments were carried out on former arable land, each in the proximity of a reference area bearing a similar complex of threatened sandy grasslands (experiment 1: fine‐scale; experiment 2: landscape scale). In both experiments we used nutrient‐poor deep‐sand substrate (abiotic approach), raked/mown inoculation material from target areas and grazing as management tool (biotic and management approach). The vegetation of the restoration and donor areas was sampled once a year and analysed by non‐metric multidimensional scaling (NMDS) ordination and target‐species ratios. Mixed linear models were calculated to determine effects of grazing (experiment 1) and year (both experiments).Results: NMDS revealed a continuous development of the restored sites towards the corresponding donor sites. Similarly, target‐species ratios of the restored sites tended towards the ratios of the donor sites. To date, grazing effects have mainly been structural: reduction of a carpet‐forming pleurocarpous moss species and of litter. In addition, cover of target species in relation to total plant cover was significantly enhanced by grazing in the last two study years.Conclusions: The combination of nutrient‐poor substrate, inoculation with raked/mown plant material and grazing proved to be a very effective restoration method for dry base‐rich sand ecosystems. After 4 yr the restored plant communities serve as well‐developed parts of a habitat network.
A new bright green‐emitting fluorescent protein – engineered monomeric and dimeric forms
Authors: Ilagan Robielyn P. , Rhoades Elizabeth , Gruber David F. , Kao Hung‐Teh , Pieribone Vincent A. , Regan Lynne 
Published: March 2010

Fluorescent proteins have become essential tools in molecular and biological applications. Here, we present a novel fluorescent protein isolated from warm water coral,Cyphastrea microphthalma. The protein, which we named vivid Verde fluorescent protein (VFP), matures readily at 37 °C and emits bright green light. Further characterizations revealed that VFP has a tendency to form dimers. By creating a homology model of VFP, based on the structure of the red fluorescent protein, DsRed, we were able to make mutations that alter the protein’s oligomerization state. We present two proteins, mVFP and mVFP1, that are both exclusively monomeric, and one protein, dVFP, which is dimeric. We characterized the spectroscopic properties of VFP and its variants in comparison with enhanced green fluorescent protein (EGFP), a widely used variant of GFP. All the VFP variants are at least twice as bright as EGFP. Finally, we demonstrated the effectiveness of the VFP variants in bothin vitroandin vivodetection applications.Structured digital abstractMINT‐7709188:VFP (uniprotkb:D1J6P8) and VFP (uniprotkb:D1J6P8) bind (MI:0407) byclassical fluorescence spectroscopy (MI:0017)MINT‐7709201:VFP (uniprotkb:D1J6P8) and VFP (uniprotkb:D1J6P8) bind (MI:0407) byfluorescence correlation spectroscopy (MI:0052)MINT‐7709216, MINT‐7709247, MINT‐7709237:VFP (uniprotkb:D1J6P8) and VFP (uniprotkb:D1J6P8) bind (MI:0407) by molecular sieving (MI:0071)
Nutritional Dimorphism in New Guinea Dioecious Figs
Authors: Weiblen George D. , Lomascolo Silvia B. , Oono Ryoko , Dumont Elizabeth R. 
Published: March 2010

ABSTRACTThe life history of figs (Ficus, Moraceae) involves pollination by specialized insects and seed dispersal by vertebrate frugivores. This three‐way interaction raises the possibility of conflict between pollinators and seed dispersers over fig resources. The conflict might be mediated in dioecious figs by the segregation of inflorescences with specialized male and female sexual functions, termed gall figs and seed figs, on separate trees. In dioecious figs, pollinators are intimately associated with gall figs, whereas vertebrate frugivores prefer seed figs and disperse the seeds they contain. Optimal foraging theory predicts that frugivore preference for ripe seed figs is associated with superior nutritional quality when compared to gall figs. We tested this prediction comparing nutrient and mineral properties of ripe figs in 12 functionally dioecious and two monoecious species from New Guinea. Analyses of variance indicated that gall figs contain more fiber and minerals, whereas seed figs contain significantly more carbohydrates and fat. Fruit bats, the primary dispersers of dioecious figs in New Guinea, prefer carbohydrate‐rich diets, and from this perspective, seed figs offer a greater nutritional reward than gall figs. More nondigestible fiber in gall figs than seed figs would appear to discourage frugivory. Parallel differences between ripe gall figs and seed figs occur in each independent dioecious lineage whereas nutritional content in monoecious figs is more similar to seed figs. This suggests that sexual dimorphism in nutritional quality might be adaptive and associated with the maintenance of functional dioecy in figs.
Are We Losing the Best Parts of Our Protected Areas in Tropical Mountains?
Authors: Chai Shauna‐Lee , Tanner Edmund 
Published: March 2010

ABSTRACTProtected areas (PAs) on tropical mountains undergo greater forest destruction in their lower altitudes. We compared the extent of forested, nonforested, and fragmented areas between lowland (<1000 m asl) and montane zones of the Blue Mountains inside the Blue and John Crow Mountains National Park established in Jamaica in 1993. We found that in 2008, inside the montane zone, only 4 percent of forest was cleared, and forest fragmentation was minimal. In the lowland zone, however, the percentage of forest cleared was seven times as high, and the density of fragments was 11‐fold higher. We established twenty‐five 0.04 ha lowland plots; ordination of tree species composition in these plots reflected a rainfall gradient, showing that plots on the wetter northern side of the Blue Mountains were floristically different from those on the drier southern side. The conservation value of the remaining lowland forest is high because of its high endemism (18% of species in our plots) and beta diversity. In addition, IUCN Red List data show that about 71 percent of threatened tree species in the Blue Mountains grow in the lowland region, 92 percent of which are endemic. From these findings, we identify a ‘protected area hotspot zone’, which lies between the PA boundary and the core high‐altitude zone, and which should be instituted in IUCN categories I and II PAs.
RHEOLOGICAL AND PHYSICAL PROPERTIES OF DATE JUICE PALM BY‐PRODUCT (PHOENIX DACTYLIFERA L.) AND COMMERCIAL XANTHAN GUMS
Authors: SALAH RIADH BEN , BESBES SOUHAIL , CHAARI KACEM , RHOUMA ALI , ATTIA HAMMADI , DEROANNE CLAUDE , BLECKER CHRISTOPHE 
Published: March 2010

ABSTRACT Rheological and thermal properties of xanthan produced by Xanthomonas campestris from date by‐products (DBP‐xanthan) and commercial xanthan were determined. DBP‐xanthan gave solutions with lower apparent viscosity than commercial xanthan. However, DBP‐xanthan solutions were more stable to temperature changes and ionic strength than commercial xanthan. Gradual increase of storage modulus (G′) as function of frequency was observed for the two polysaccharides. On the other hand, loss modulus (G″) remained constant for DBP‐xanthan and an increase was observed for commercial xanthan. Granulometric profile indicates that DPB‐xanthan particle size was lower than commercial xanthan. Differential scanning calorimetry showed that the melting temperatures (Tm) and enthalpy (ΔHm) of DBP‐xanthan were higher than those of commercial xanthan. These properties of DBP‐xanthan are quite rare among xanthan described in the literature and give this new gum great potential for use in the field of bioindustries as thickening and stabilizing agents.PRACTICAL APPLICATIONSXanthan gum from date by‐products (DBP‐xanthan) was recently produced in our laboratory. No work has been undertaken to study the rheological properties of this polysaccharide to date. Rheological and some physicals properties were compared to those of commercial xanthan. This could promote industrial use of DBPs as low‐cost natural source for xanthan gum production.
A COMPARATIVE STUDY ON PHYSICAL PROPERTIES AND CHEMICAL INTERACTIONS OF GELS FROM TILAPIA MEAT PASTES INDUCED BY HEAT AND PRESSURE
Authors: TAN FA‐JUI , LAI KUNG‐MING , HSU KUO‐CHIANG 
Published: March 2010

ABSTRACT The changes of physical properties and chemical interactions of gels from tilapia meat pastes applied to hydrostatic pressure (50–300 MPa; 4C; 30 min) or heat (30–70C; 30 min) treatments were investigated. The control gel (cooking at 90C for 20 min) was elastic and white. The meat paste treated by heat treatment began to form a gel texture at 50C, and the gel was elastic, rigid, white and mainly constituted by covalent bonds. Gels induced by pressure (above 200 MPa) were softer, more viscous than those by heat, meanwhile, they were mainly constituted by hydrogen bonds and hydrophobic interactions. Subsequent cooking process (90C; 20 min) would increase the hardness, elasticity and whiteness of the gels both induced by heat and pressure treatments. The appearance of the pressure‐induced gels showed glossiness and close to native color of meat pastes whereas that of the heat‐induced gels showed white but no luster. PRACTICAL APPLICATIONSThe textural, chemical and physical characteristics of protein gels induced by heat and pressure are quite different; therefore combinations of heat and pressure treatments may produce various products. Pressure‐induced gels are mainly constituted by noncovalent bonds and are soft, viscous and glossy in appearance, whereas heat‐induced gels are mainly constituted by covalent bonds and are elastic, rigid and white. Different effects of combinations of heat and pressure treatments on protein gelation are applicable to food industries to manufacture various surimi products.
Gene flow in malaria vector Anopheles stephensi (Diptera: Culicidae) across the Aravalli Hills in North‐west India
Authors: VIPIN  , DUBE Madhulika , GAKHAR Surendra Kumar 
Published: March 2010

AbstractThe population structure of An. stephensi in North‐west India was studied to assess the impact of the Aravalli Hills, as a barrier to gene flow using microsatellite markers. Large and significant genetic differentiation was found along the sides of, as well as across, the Aravalli Hills as the mean FST and RST on west vs. east of the Aravalli Hills were 0.213, 0.112 and 0.179, 0.056, respectively. Similarly, across the hills, mean values of FST and RST were 0.100 and 0.094, respectively. Genetic diversity on both sides did not vary significantly. The FST values were more sensitive than RST values, indicating that genetic drift might have caused genetic differentiation between populations. A positive correlation (r = 0.0149 and 0.157, respective to FST and RST) was found between genetic differentiations and geographic distances irrespective of the hills. Low level of gene flow was found along both sides (Nm = 0.92 and 0.14; west vs. east of Aravalli Hills, respectively) as compared to across the Aravalli Hills (Nm = 2.25). It was found that the Aravalli Hills are not working as an effective barrier to gene flow for An. Stephensi, maybe because of the low average height and discontinuous hills, however, the distance is playing a major role for differentiation between populations due to active mode of dispersal of An. stephensi mosquitoes which have a short flight range. All this information should help draw the strategies for genetic control of mosquitoes using transgenic mosquitoes.
A three‐dimensional model of the U1 small nuclear ribonucleoprotein particle
Authors: SOMARELLI Jason A. , MESA Annia , ROY Ambrish , ZHANG Yang , HERRERA Rene J. 
Published: March 2010

AbstractMost of the pre‐mRNAs in the eukaryotic cell are comprised of protein‐coding exons and non‐protein‐coding introns. The introns are removed and the exons are ligated together, or spliced, by a large, macromolecular complex known as the spliceosome. This RNA‐protein assembly is made up of five uridine‐rich small nuclear RNAs (U1‐, U2‐, U4‐, U5‐ and U6‐snRNA) as well over 300 proteins, which form small nuclear ribonucleoprotein particles (snRNPs). Initial recognition of the 5′ exon/intron splice site is mediated by the U1 snRNP, which is composed of the U1 snRNA as well as at least ten proteins.By combining structural informatics tools with the available biochemical and crystallographic data, we attempted to simulate a complete, three dimensional U1 snRNP from the silk moth, Bombyx mori. Comparison of our model with empirically derived crystal structures and electron micrographs pinpoints both the strengths and weaknesses in the in silico determination of macromolecular complexes. One of the most striking differences between our model and experimentally generated structures is in the positioning of the U1 snRNA stem‐loops. This highlights the continuing difficulties in generating reliable, complex RNA structures; however, three‐dimensional modeling of individual protein subunits by threading provided models of biological significance and the use of both automated and manual docking strategies generated a complex that closely reflects the assembly found in nature. Yet, without utilizing experimentally‐derived contacts to select the most likely docking scenario, ab initio docking would fall short of providing a reliable model. Our work shows that the combination of experimental data with structural informatics tools can result in generation of near‐native macromolecular complexes.