bluuebunny/crossref_metadata_2025_split huggingface dataset:
The prevalence and assessment of ErbB2‐positive breast cancer in Asia
Authors: Tan Yew Oo , Han Sehwan , Lu Yen‐Shen , Yip Cheng‐Har , Sunpaweravong Patrapim , Jeong Joon , Caguioa Priscilla B. , Aggarwal Shyam , Yeoh Ee Min , Moon Hanlim 
Published: August 2010

AbstractOverexpression of the epidermal growth factor receptor‐related gene ErbB2 occurs in 18% to 25% of patients with breast cancer in Western countries and is associated with a poor prognosis. The prevalence of ErbB2‐positive tumors in Asia is unclear, partly because data are limited. The objective of this review was to summarize the reported prevalence of ErbB2‐positive tumors from a large sample of Asian patients and to examine ErbB2 assessment methods in Asia. From searches of MEDLINE, local language journals, and local and international conference proceedings as well as locoregional breast cancer experts' recommendations, the authors selected up to 5 studies each from India, Korea, Malaysia, the Philippines, Singapore, Taiwan, and Thailand that reported ErbB2 results based on assessment with immunohistochemistry (IHC) and/or fluorescence in situ hybridization (FISH). The reported prevalence of ErbB2‐positive tumors in 22 studies on 24,671 patients, of whom 14,398 patients were assessed for ErbB2 status, varied widely (range, 6%‐65%) as did the assessment methods used. Most studies (n = 21) used IHC to assess ErbB2 status, but definitions for positivity varied. When robust assessment methods were used, the median prevalence was 19% based on strong IHC staining (IHC3+; n = 9812 patients) and 25% based on FISH (n = 681 patients). Data on the prevalence of ErbB2‐positive breast cancer in Asia are limited. The current survey indicated that the prevalence in Asia may be similar to that in Western countries; thus, up to 1 in 4 Asian patients with breast cancer potentially could benefit from ErbB2‐targeted treatment. A standard, reliable ErbB2 assessment method available to patients across Asia is urgently required. Cancer 2010. © 2010 American Cancer Society.
The frameshift polymorphismCYP3A43_74_delA is associated with poor differentiation of breast tumors
Authors: Justenhoven Christina , Winter Stefan , Hamann Ute , Haas Susanne , Fischer Hans‐Peter , Pesch Beate , Brüning Thomas , Ko Yon‐Dschun , Brauch Hiltrud ,   for the GENICA Network
Published: August 2010

AbstractBACKGROUND:CYP3A enzymes, due to their role in the metabolism of steroid hormones, are suggested to affect carcinogenesis of hormone‐related cancers. The purpose of the present study was to evaluate the association between polymorphisms located inCYP3A43, breast cancer risk, and tumor characteristics.METHODS:A 3‐plex matrix‐assisted laser desorption ionization time of flight mass spectrometry assay has been established forCYP3A43_74_delA (CYP3A43*2A),CYP3A43_1018_C>G (CYP3A43*3), andCYP3A43_1047_C>T (CYP3A43*1B) polymorphisms, and 1021 breast cancer cases and 1015 age‐matched, population‐based controls from the German GENICA collection have been genotyped.RESULTS:No differences in genotype frequencies between cases and controls were observed, indicating thatCYP3A43_74_delA is not associated with breast cancer risk. Subgroup analyses showed an association between theCYP3A43_74_delA allele and high‐grade tumors (odds ratio, 1.74; 95% confidence interval, 1.14‐2.65 [P= .010 andPtrend= .012]).CONCLUSIONS:The data support the notion that theCYP3A43_74_delA variant may result in decreased protein and/or activity levels, and this may further lead to increased hormone levels to promote tumor cell growth and hinder differentiation. Cancer 2010. © 2010 American Cancer Society.
Histopathologic characteristics of early stage esophageal carcinoma: A comparative study with gastric carcinoma
Authors: Goseki Narihide , Koike Morio , Yoshida Misao 
Published: August 2010

AbstractUnlike the stomach, the esophageal mucosal layer has abundant vessels. To study the histopathologic character of cancer of the esophagus in comparison with that of stomach, the histologic findings (such as vascular or lymphatic permeation and lymph nodal involvement of cancer) were surveyed in 52 lesions of superficial esophageal carcinoma in which carcinoma invasion was limited to the submucosal layer, 448 cases of so‐called early gastric carcinoma in which the carcinoma invasion was limited to the submucosal layer, and 109 cases of gastric carcinoma invading the proper muscle layer (PM‐carcinoma), With respect to lymph node metastasis and lymphatic and vascular permeation, there was a particularly high incidence of carcinoma extending to the submucosal layer (SM‐carcinoma) of the esophagus and a similar high incidence of PM‐carcinoma of the stomach. Carcinoma limited to the mucosal epithelium and mucosal layer of the esophagus (MM‐carcinoma) has an incidence similar to carcinoma in the mucous membrane and SM‐carcinoma of the stomach, respectively. Thus, the cases in which carcinoma invasion was limited to the muscle layer of the mucosa of the esophagus were shown to have a prognosis similar to that of so‐called early carcinoma of the stomach.
HLA‐DR antigen expression in intestinal‐type and diffuse‐type gastric carcinoma
Authors: Teh Ming , Lee Yoke‐Sun 
Published: August 2010

AbstractThe expression of the HLA‐DR antigen, a Class II HLA antigen, was analyzed in 41 cases of gastric carcinoma (23 intestinal‐type and 18 diffuse‐type according to a modified Lauren classification) using a monoclonal antibody that is reactive to this antigen in routinely formalin‐fixed, paraffin‐embedded tissue sections. Seventeen cases of intestinal‐type gastric carcinoma and two cases of diffuse‐type gastric carcinoma were positive for the HLA‐DR antigen. The staining in intestinal‐type carcinoma generally was stronger and more extensive than in diffuse‐type carcinoma, which showed only weak and focal staining. The difference in the frequency and pattern of staining between intestinal and diffuse‐type gastric carcinoma supports the concept that these are two distinct subtypes of gastric carcinoma.
Clinical and histopathologic evaluation of the expression of Ha‐ras and fes oncogene products in lung cancer
Authors: Nishio Hiroshi , Nakamura Shin‐Ichiro , Horai Takeshi , Ikegami Harumichi , Matsuda Minoru 
Published: August 2010

AbstractThe expression of Ha‐ras and fes oncogenes was investigated with the immunohistochemical method in formalin‐fixed, paraffin‐embedded tissue specimens of 147 lung carcinomas. Positive immunoperoxidase reactions for Ha‐ras p21 were found in 80.5% of the adenocarcinomas, 39.5% of the squamous cell carcinomas, 21,4% of the large cell carcinomas, and 15.4% of the small cell carcinomas; those for fes P85 were found in 51.2% of the adenocarcinomas, 26.3% of the squamous cell carcinomas, 35.7% of the large cell carcinomas, and 15.4% of the small cell carcinomas. Both Ha‐ras p21 and fes P85 were expressed most frequently and most strongly in adenocarcinoma. In addition, adenocarcinoma showed significantly higher incidence of concomitant expression of Ha‐ras p21 and fes P85 as compared with other histologic types of lung cancer. Thus, the authors suggest that the cooperative effects of Ha‐ras and fes oncogenes are especially important in the carcinogenesis of adenocarcinoma. In adenocarcinoma, the incidence and grade of Haras p21 expression increased with the degree of histologic differentiation, suggesting that Ha‐ras oncogene might be related to cellular differentiation. Papillary adenocarcinoma showed more frequent Ha‐ras p21 expression in comparison with acinar adenocarcinoma. In well‐or moderately differentiated adenocarcinoma, the incidence and grade of Ha‐ras p21 immunoreactivity in the cases with poor prognosis were significantly higher than in those with good prognosis if other major prognostic factors were equivalent in the two groups. The authors propose that the expression of Ha‐ras p21 may be one of the useful prognostic factors in such carcinomas.
Primary treatment of pelvic osteosarcoma: Report of five cases
Authors: Estrada‐Aguilar J. , Greenberg H. , Walling A. , Schroer K. , Black T. , Morse S. , Hvizdala E. 
Published: August 2010

AbstractFive patients, ages 12 to 20 years, with nonresectable primary (Patients 2, 3, and 5) and metastatic (Patients 1 and 4) pelvic osteosarcomas were treated with intraarterial cisplatin and concurrent radiation therapy from 1983 to 1987. Long‐term local tumor control was achieved in all five patients. Patients 1 and 3 are alive with no evidence of local recurrence or metastatic disease at 77 and 56 months of follow‐up, respectively, since diagnosis of the pelvic tumor. Patients 2, 4, and 5 died of metastatic lung disease at 25, 39, and 12 months, respectively, after diagnosis of the pelvic tumor. Patient 4 had no clinical or radiologic evidence of local recurrence. Control of tumor growth in patients with pelvic osteosarcomas can be achieved with regional chemotherapy and concurrent radiation therapy. These patients also should receive adjuvant intensive systemic chemotherapy to increase the probability of eliminating potential subclinical metastatic disease.
Immunohistochemical demonstration of elevated expression of epidermal growth factor receptor in the neoplastic changes of cervical squamous epithelium
Authors: Maruo Takeshi , Yamasaki Masaaki , Ladines‐Llave Cecilia A. , Mochizuki Matsuto 
Published: August 2010

AbstractTo evaluate epidermal growth factor (EGF) receptor expression in the neoplastic process of squamous cell epithelium of the uterine cervix, normal, premalignant, and malignant cervical tissues were examined for the presence of EGF receptor by the avidin‐biotin immunoperoxidase techniques with a monoclonal antibody to EGF receptor, Although normal cervical epithelium did not show appreciable staining for EGF receptor, predominant staining for the receptor was observed in most dys‐plastic epithelia and carcinomas in situ. In invasive squamous carcinoma, there was a great difference in the immunohistochemically detected levels of EGF receptor among the histologic cell types. Large cell nonkeratinizing carcinoma and its keratinizing counterpart contained high levels of EGF receptor; small cell nonkeratinizing carcinoma lacked immunostainable EGF receptor, These results suggest that the elevated expression of EGF receptor may be involved in the initial stage of tumori‐genesis of cervical squamous epithelium and that EGF receptor expression may be related to the differentiation or dedifferentiation of cervical squamous carcinoma cells.
Blood eosinophilia in hodgkin's disease: A follow‐up of 25 cases in Venezuela
Authors: Desenne Jean J. , Acquatella Greta , Stem Ronald , Mullet Aixa , Sanchez Mario , Somoza Rosita 
Published: August 2010

AbstractTwenty‐five patients with Hodgkin's disease and high eosinophil counts were observed for an average of 90 months. Fluctuations in the levels of eosinophils were important in the course of observation. No relation with stages, histologic type, or evolution was noticed. Steroid‐containing regimens and severe premortem conditions seemed to lower the counts. Relapse‐free survival was shorter in our 25 patients than in a control group of 50 patients with Hodgkin's disease and no eosieophilia who had approximately the same stage, histologic type, and treatment of disease. However, the overall survival was somewhat better for the eosinophilic patients with stages MB and IV (0.1 > P > 0.05).
Clinicopathologic and therapeutic aspects of angioimmunoblastic lymphadenopathy‐related lesions
Authors: Ohsaka Akimichi , Saito Ken , Sakai Takao , Mori Shigeo , Kobayashi Yukio , Amemiya Youichi , Sakamoto Shinobu , Miura Yasusada 
Published: August 2010

AbstractThe clinicopathologic features of 14 patients with angioimmunoblastic lymphadenopathy (AIL)‐related lesions were analyzed. Lymph node biopsy specimens from all the patients showed a diffuse obliteration of lymph node architecture, prominent vascular proliferation, a polymorphous cellular infiltrate, including immuno‐blasts, and varying degrees of clear cell proliferation. The patients were eight males and six females, with a median age of 58.5 years. All but one were in an advanced stage at the time of diagnosis. Bone marrow involvement was observed in eight patients. Thirteen patients had a negative serologic reaction for antibody to human T‐cell leukemia virus type I (HTLV‐I), and one patient was considered to be a HTLV‐I carrier. Polyclonal hypergammaglobulinemia was observed in 6 patients, and 6 of the 12 patients showed elevated IgE levels. Immunophenotyping of the involved lymph nodes revealed a preponderance of T‐cells in all the patients. Eleven of these patients showed a predominance of CD4+ over CD8+ T‐cells, and only one patient showed a predominance of CD8+ over CD4+ T‐cells. Two of five patients whose gene analysis was carried out showed clonal rearrangement of the T‐cell receptor beta chain gene without rearrangement of the immunoglobulin heavy chain genes. Twelve patients received doxorubicin‐containing combination chemo therapy; of these, 7 patients achieved complete response, and the other 5 had partial response. Nine patients are still alive with a median follow‐up period of 21 months, and five patients died during the follow‐up period. Progression to high‐grade T‐cell lymphoma with systemic infiltration was ascertained in two of three cases for which autopsy was performed. From our experience, we recommend doxorubicin‐containing combination chemotherapy as initial therapy for AIL‐related lesions.
Childhood cancer registry of the province of Torino, Italy: Survival, incidence, and mortality over 20 years
Authors: Mosso Maria Luisa , Colombo Renata , Giordano Livia , Pastore Guido , Terracini Benedetto , Magnani Corrado 
Published: August 2010

AbstractIncident childhood cancers in the Province of Torino, Italy (population aged 0 to 14 years averaging 0,5 million) have been registered since 1967. Four population‐based exhaustive surveys have been done to collect cases diagnosed during the periods 1967 to 1969, 1970 to 1975, 1976 to 1981, and 1982 to 1986. For each registered child, vital status on June 30, 1988 was assessed. This article reports incidence, mortality, and survival rates over a 20‐year period. A statistically significant trend toward an increased incidence of soft tissue sarcomas was identified. Statistically insignificant trends included an increased incidence of brain tumors (probably reflecting improved diagnostic procedures) and a decreased incidence of thyroid tumors. Incidence rates of leukemias in the first year of life tended to decrease. As expected, survival rates of some childhood cancers dramatically improved throughout the 20‐year period; this occurred in leukemias, brain tumors, soft tissue sarcomas, and renal tumors. Survival rates are compared with observations in comparable population‐based series.
In situ X‐ray Absorption Analysis of ∼1.8 nm Dendrimer‐Encapsulated Pt Nanoparticles during Electrochemical CO Oxidation
Authors: Weir Michael G. , Myers V. Sue , Frenkel Anatoly I. , Crooks Richard M. 
Published: August 2010

AbstractWe report an in situ X‐ray absorption‐fine structure (XAFS) spectroscopic analysis of ∼1.8 nm Pt dendrimer‐encapsulated nanoparticles (DENs) during electrocatalytic oxidation of CO. The results indicate that Pt nanoparticles encapsulated within poly(amidoamine) (PAMAM) dendrimers and immobilized on a carbon electrode retain their electrocatalytic activity and are structurally stable for extended periods during CO oxidation. This is a significant finding, because nanoparticles in this size range are good experimental models for comparison to first‐principles calculations if they remain stable.
Historischer Abriss zur Entwicklung des Nachhaltigen Bauens
Authors: Vogdt Frank U. , Piesker Julia 
Published: August 2010

AbstractDer Artikel beschäftigt sich mit der historischen Entwicklung des Nachhaltigkeitsgedankens und zeigt die sich daraus ergebenden Anforderungen und Qualitäten für das Nachhaltige Bauen auf. Ökonomische, ökologische und soziokulturelle Aspekte sind im Rahmen des Nachhaltigen Bauens über den gesamten Lebenszyklus eines Gebäudes “von der Wiege bis zur Bahre” gleichwertig zu betrachten. Daraus ergeben sich vielfältige Wechselwirkungen und Abhängigkeiten, die mit den Methoden einer ganzheitlichen Planung optimiert werden können. Für verschiedene ökologische, ökonomische und soziokulturelle Aspekte werden exemplarisch diese Abhängigkeiten und die sich daraus ergebenden Konsequenzen beschrieben.Historical development of sustainable building. This article deals with the historical development of sustainability and it depicts on the resulting requirements und qualities for sustainable building. Ecological, economical und socio‐cultural aspects have to be regarded as equal considering the whole life‐cycle of the building from ”cradle to grave”. Due to this several interdependencies arise that can be optimised by means of ecological and economical life cycle analysis. Regarding several ecological, economical und socio‐cultural aspects this article identifies those dependencies and reveals the resulting implications.
Evidence for multiple, developmentally regulated isoforms of Ptprq on hair cells of the inner ear
Authors: Nayak Gowri , Goodyear Richard J. , Legan P. Kevin , Noda Masaharu , Richardson Guy P. 
Published: August 2010

AbstractPtprq is a receptor‐like inositol lipid phosphatase associated with the shaft connectors of hair bundles. Three lines of evidence suggest Ptprq is a chondroitin sulfate proteoglycan: (1) chondroitinase ABC treatment causes a loss of the ruthenium‐red reactive, electron‐dense particles associated with shaft connectors, (2) chondroitinase ABC causes an increase in the electrophoretic mobility of Ptprq, and (3) hair bundles in the developing inner ear of wild‐type mice, but not those of Ptprq−/− mice, react with monoclonal antibody (mAb) 473‐HD, an IgM that recognizes the dermatan‐sulfate‐dependent epitope DSD1. Two lines of evidence indicate that there may be multiple isoforms of Ptprq expressed in hair bundles. First, although Ptprq is expressed throughout the lifetime of most hair cells, hair bundles in the mouse and chick inner ear only express the DSD1 epitope transiently during development. Second, mAb H10, a novel mAb that recognizes an epitope common to several avian inner‐ear proteins including Ptprq, only stains mature hair bundles in the extrastriolar regions of the vestibular maculae. MAb H10 does not stain mature hair bundles in the striolar regions of the maculae or in the basilar papilla, nor does it stain immature hair bundles in any organ. Three distinct, developmentally regulated isoforms of Ptprq may therefore be expressed on hair bundles of the chick inner ear. Hair bundles in the mature chick ear that do not express the H10 epitope have longer shaft connectors than those that do, indicating the presence or absence of the H10 epitope on Ptprq may modulate the spacing of stereocilia. © 2010 Wiley Periodicals, Inc. Develop Neurobiol 71: 129‐141, 2011
Progesterone, administered before kainic acid, prevents decrements in cognitive performance in the Morris Water Maze
Authors: Frye Cheryl A. , Walf Alicia 
Published: August 2010

AbstractThe nature of progesterone (P4)'s neuroprotective effects is of interest. We investigated effects of P4 when administered before, or after, kainic acid, which produces ictal activity and damage to the hippocampus, to mediate effects on spatial performance. The hypothesis was that P4, compared with vehicle, would reduce decrements in Morris Water Maze performance induced by kainic acid. Experiment 1: We examined the effects of kainic acid on plasma stress hormone, corticosterone, and progestogen (P4 and its metabolites) levels in plasma and the hippocampus after subcutaneous (s.c.) P4 administration to ovariectomized rats. Rats administered kainic acid had the highest corticosterone levels immediately following injection. P4 is 5α‐reduced to dihydroprogesterone (DHP) and subsequently metabolized to 5α‐pregnan‐3α‐ol‐20‐one (3α,5α‐THP) by 3α‐hydroxysteroid dehydrogenase. The regimen of P4 used produced circulating and hippocampal levels of P4, DHP, and 3α,5α‐THP within a physiological range, which declined at 14 hours postinjection and were not altered by kainic acid. Experiment 2: The physiological P4 regimen was administered to rats before, or after, kainic acid‐induced seizures, and later effects on water maze performance were compared with that of rats administered vehicle. Rats administered kainic acid had significantly poorer performance in the water maze (i.e., increased latencies and distances to the hidden platform) than did rats administered vehicle. Administration of P4 before, but not after, kainic acid prevented these performance deficits. Thus, these data suggest that a physiological regimen of P4 can prevent some of the deficits in water maze performance produced by kainic acid. © 2010 Wiley Periodicals, Inc. Develop Neurobiol 71: 142‐152, 2011
Tamoxifen‐inducible Cre‐mediated recombination in adipocytes
Authors: Sassmann Antonia , Offermanns Stefan , Wettschureck Nina 
Published: August 2010

AbstractTo generate a mouse line which allows inducible, Cre/loxP‐dependent recombination in adipocytes, we used RedE/RedT‐mediated recombineering to insert the CreERT2‐transgene, which encodes a fusion protein of Cre and a mutated tamoxifen‐responsive estrogen receptor, into the start codon of the adipocyte‐specific Adipoq gene. Adipoq encodes adiponectin, an adipokine specifically expressed in differentiated adipocytes. Tamoxifen treatment induced almost complete recombination in white adipose tissue of the AdipoqCreERT2 mouse line (97%–99%), while no recombination was seen in vehicle‐treated animals. Recombination in brown adipose tissue was about 15%, whereas other organs and tissues did not undergo recombination. In addition, mice expressing CreERT2 in adipocytes did not show any alterations of metabolic functions like glucose tolerance, lipolysis, or energy expenditure compared to control mice. Therefore the AdipoqCreERT2 mouse line will be a valuable tool for studying the consequences of a temporally controlled deletion of floxed genes in white adipose tissue. genesis 48:618–625, 2010. © 2010 Wiley‐Liss, Inc.
RU486‐inducible recombination in the salivary glands of lactoferrin promoter‐driven green fluorescent Cre transgenic mice
Authors: Chen Mei‐Ru , Liu Shan‐Wen , Wu Tzu‐Chin , Kao Vivia Y. , Yu Hsiao‐Chi , Chen Fidji H. , Hsu Ching‐Han , Chen Chin‐Tu , Lin Kurt M. 
Published: August 2010

AbstractWhen compared with the many tamoxifen‐activated Cre mouse lines available for gene manipulation studies, relatively few RU486‐inducible Cre mice are in use, due to leakiness issues. Here, we report the generation of an RU486‐inducible triple fusion gene (GCrePR1e), consisting of green fluorescent protein, Cre, and the progesterone receptor ligand‐binding domain (F642‐L901). We sought to improve the GCrePR1e by selecting a truncated human lactoferrin (Lf) promoter to drive its expression, based on the promoter's low basal activity and innate sensitivity to RU486. The resulting vector displayed decreased leakiness and increased Cre induction by RU486 through transcriptional and posttranslational regulation in in vitro transfection assays. Inducible GCrePR1e expression was found in most organs of Lf‐GCrePR1e transgenic mice and highly activated in the salivary gland, spleen, and lymph nodes. In the bigenic mouse generated by crossing the Lf‐GCrePR1e mouse and the Cre reporter mouse (R26R‐LacZ), we found that RU486‐induced LacZ expression only in the mucous acini and striated ducts of the salivary gland and had very low background recombination in the untreated mice. Our results demonstrated that the Lf‐CrePR1e vector was suitable for in vitro recombination in culture models, and Lf‐CrePR1e transgenic mice could mediate spatially restricted and RU486‐induced gene manipulation in the salivary gland. genesis 48:585–595, 2010. © 2010 Wiley‐Liss, Inc.