bluuebunny/crossref_metadata_2025_split huggingface dataset:
FBXO45 levels regulated ferroptosis renal tubular epithelial cells in a model of diabetic nephropathy by PLK1
Authors: Zhu Bingming , Hu Yongxuan , Wu Ruishan , Yu Quan , Wen Wangrong 
Published: May 2024

Abstract

Objective
This research aims to investigate the role and underlying biological mechanism of FBXO45 in regulating ferroptosis of renal fibrocytes in a diabetic nephropathy (DN) model.

Methods
C57BL/6 mice were fed with a high-fat diet and injected with streptozotocin to induce diabetes. Human renal glomerular endothelial cells stimulated with d-glucose.

Results
Serum FBXO45 mRNA expression was found to be down-regulated in patients with DN. There was a negative correlation between the expression of serum FBXO45 mRNA and serum α-SMA, Collagen I, and E-cadherin mRNA in patients with DN. Additionally, the expression of serum FBXO45 mRNA showed a negative correlation with blood sugar levels. Based on a 3D model prediction, it was observed that FBXO45 interacts with polo-like kinase 1 (PLK1) at GLY-271, ILE-226, GLY-166, LEU-165, ARG-245, and ASN-220, while PLK1 interacts with FBXO45 at TYR-417, ARG-516, HIS-489, TYR-485, GLN-536, and ARG-557. This interaction was confirmed through immunoprecipitation assay, which showed the interlinking of FBXO45 protein with PLK1 protein.

Conclusions
These findings indicate that FBXO45 plays a role in mitigating ferroptosis in DN through the regulation of the PLK1/GPX4/SOX2 pathway. This highlights the potential of targeting FBXO45 as a therapeutic approach to ameliorate ferroptosis in DN.
Compressive and tensile strength estimation of sustainable geopolymer concrete using contemporary boosting ensemble techniques
Authors: Zhou Ji , Tian Qiong , Ahmad Ayaz , Huang Jiandong 
Published: May 2024

Abstract
Geopolymer concrete (GPC) serves as an environmentally conscious alternative to traditional concrete, offering a sustainable solution for construction needs. The ability to make on-site changes is dependent on the concrete’s strength after casting, which must be higher than the target value. To anticipate the concrete’s strength before it is poured is, thus, quite helpful. Three ensemble machine learning (ML) approaches, including gradient boosting, AdaBoost regressor, and extreme gradient boosting, are presented in this work as potential methods for forecasting GPC’s mechanical strength that incorporates corncob ash. To determine which modeling parameters are crucial, sensitivity analysis was employed. When the compressive strength and split-tensile strength of GPC were tested with ensemble ML models, R
2 values of more than 90% were discovered between the predicted and actual results. Statistics and a k-fold analysis based on the error and coefficient of determination were used to verify the developed models. Slag amount, curing age, and fine aggregate quantity were the three mix proportions that had the most impact on GPC’s mechanical strength, as shown in the sensitivity analysis. The results of this study demonstrated that ensemble boosting approaches could reliably estimate GPC mechanical strength. Incorporating such procedures into GPC quality control can yield significant improvements.
GRSF1 deficiency attenuates mitochondrial function in aging granulosa cells
Authors: Wen Canxin , Jiang Linlin , Pan Ping , Huang Jia , Xie Yanxin , Ou Songbang , Li Yu 
Published: May 2024

In brief
Ovarian aging results in reactive oxygen species accumulation and mitochondrial deterioration. During the aging process, GRSF1 deficiency attenuates mitochondrial function in aging granulosa cells.

Abstract
Ovarian aging critically influences reproductive potential, with a marked decrease in oocyte quality and quantity and an increase in oxidative stress and mitochondrial dysfunction. This study elucidates the role of guanine-rich RNA sequence binding factor 1 (GRSF1) in the aging of ovarian granulosa cells (GCs). We observed a significant reduction in GRSF1 within GCs correlating with patient age, utilizing clinical samples from IVF patients. Using an siRNA-mediated knockdown technique, we established that diminished GRSF1 expression exacerbates mitochondrial dysfunction, elevates reactive oxygen species, and impairs ATP production. Furthermore, RNA immunoprecipitation revealed GRSF1’s interaction with superoxide dismutase 2 (SOD2) mRNA, a key antioxidant enzyme, suggesting a mechanism whereby GRSF1 modulates oxidative stress. Downregulation of SOD2 reversed the protective effects of GRSF1 overexpression on mitochondrial function. These insights into the role of GRSF1 in ovarian aging may guide the development of interventions to improve fertility outcomes in advanced age.
Assessment of demographic attributes and health profile of schedule caste of 5 villages of Aligarh District, Uttar Pradesh, India
Authors: Akhtar Sada , Mudassir Mohd , Faiz Abul , Khan Parvez , Afza Sheereen , Ahmad Farha , Ahmad N. Zaheer 
Published: May 2024

Background: Demography is the scientific study of human populations. It takes into account the quantitative aspects of their general characteristics. In this paper health profile and different attributes of demography like age, gender, caste, marital status, educational status, temperament and occupation etc of scheduled caste people of 5 villages of Aligarh district were assessed and documented.
Methods: A pretested, predesigned questionnaire containing the demographic attributes was filled by researchers by face-to-face examination of the population through scheduled caste sub plan mobile health care OPD’s. Surveys were conducted from 2nd June to 25th September 2022.
Results: Collected and analyzed data exhibits that there were marked difference with the age groups 0-15, 26-45 and 66-85, gender, caste, with religion of Hindu (90.83%) and Muslim (7.33%), educational status regarding illiterate (59.81) and graduate or above (1.65%), Mizaj, dietary habit and there were no significant differences regarding marital status, occupation and addiction behavior were noticed.
Conclusions: It can be concluded that majority of the scheduled caste population was living in interiors of rural areas with low socio-economic and health status. Most visited gender were females which indicate poor nutrition, lack of immunity and excessive burden on them. Majority of the population belongs to Balghami Mizaj. Most preferred diets were mixed and main occupation of the scheduled caste population was agriculture. This study will make policy makers and health workers derive practical conclusions which will help in the upliftment of the scheduled caste populations.
Assessing the Quality of Shellfish Harvesting Areas: The Case of the Oualidia and Sidi Moussa Lagoons in Morocco
Authors: Khbaya K. , Bouthir F-Z. , Cherkaoui R. , Kabiri E. M. , Chfiri R. , Bessa A. 
Published: May 2024

Based on European regulation 91/492/EC, Morocco, very early on, established legislation with conditions for producing and marketing live bivalve mollusks. In applying this legislation, the National Institute of Fisheries Research (INRH)[1] has set up a system for sanitary monitoring of the marine environment, through which several harvesting areas have been classified while others are in progress. In January 2020, the Oualidia and Sidi Moussa lagoons were categorized respectively in classes B and C, with 52.77% of the results falling between 230 and 4600 MPN E. coli/100 g of flesh and intravalvular liquid (FIL) and 11.11% of the results falling between 4600 and 46000 MPN E. coli/100 g FIL. Sidi Moussa Lagoon has been classified as a category C since 2006. Consequently, oyster farming activity has been suspended in this area. This incident serves as a warning sign of the significant weakness of these ecosystems in addressing multiple social and economic challenges. On the other hand, INRH has sufficient data and tools to progress toward better optimization of the marine environment and sanitary monitoring program management.

[1] Institut National de Recherche Halieutique.
Factores De Riesgo En La Automedicación De Adultos En Santa Rosa Jáuregui, Querétaro
Authors: Olvera Alejandra Camacho , Lira Alberto Juárez 
Published: May 2024

Introducción: La automedicación es considerada como la libertad para consumir medicamentos sin prescripción médica, para el tratamiento de enfermedades o síntomas reconocidos por el paciente. Objetivo: Analizar los factores de riesgo para la automedicación en adultos que acuden al Centro de Salud de Santa Rosa Jáuregui, Querétaro. Material y métodos: Estudio transversal con enfoque cuantitativo donde el universo de estudio fueron adultos de ambos sexos, de cualquier condición de salud que acuden al centro de salud de Santa Rosa Jáuregui. Como instrumento se utilizó el “Cuestionario para los factores en la automedicación” y el “Cuestionario para la automedicación”. La muestra fue de 185 encuestados. Resultados: El 51.35 % de las personas manifiestan automedicarse por decisión propia. Los principales grupos farmacológicos usados para la práctica de automedicación son por analgésicos no esteroideos (AINES) con 86.48%, el grupo de tracto alimentario y metabolismo con igualdad de porcentaje con el grupo de antiinfecciosos para uso sistémico con el 57.29% ambos. Como principales factores de la automedicación son: factores sociodemográficos, sociales, culturales y económicos. Conclusiones: La automedicación constituye un hecho cotidiano en la mayoría de los hogares. Las personas la consideran útil debido a la mejoría de sus síntomas.

Introduction: Self-medication is considered as the freedom to consume medicines without a doctor's prescription, for the treatment of diseases or symptoms recognized by the patient. Objective: To analyze the risk factors for self-medication in adults attending the Santa Rosa Jáuregui Health Center, Querétaro. Material and methods: Cross-sectional study with a quantitative approach where the study universe was adults of both sexes, with any health condition who attend the Santa Rosa Jáuregui health center. The “Questionnaire for factors in self-medication” and the “Questionnaire for self-medication” were used as instruments. The sample was 185 respondents. Results: 51.35% of the people said that they self-medicate by choice. The main pharmacological groups used for the practice of self-medication are non-steroidal analgesics (NSAIDs) with 86.48%, the group of alimentary tract and metabolism with equal percentage with the group of anti-infectives for systemic use with 57.29% both. The main factors of self-medication are: sociodemographic, social, cultural and economic factors. Conclusions: Self-medication is a daily occurrence in most households. People consider it useful because of the improvement in their symptoms.
Special Issue on World BOSAI Forum 2023 / IDRC 2023 in SENDAI
Authors: Imamura Fumihiko , Ono Yuichi , Sasaki Daisuke , Hara Yuta 
Published: May 2024

The World BOSAI Forum 2023 (WBF2023), held in March 2023 in Sendai, Japan, was successfully convened as a global platform for practical discussion of disaster risk reduction issues in a free and dynamic manner, in contrast to the United Nations, where national interests collide, or academic conferences where experts gather. In particular, we aimed to disseminate the knowledge and lessons learned from the 2011 Great East Japan Earthquake and Tsunami (GEJET) to the world and share the knowledge and lessons with Japan. We would like to take this opportunity again to express our heartfelt gratitude to all those who participated in the sessions, exhibitions, posters, and flash talk presentations, as well as to the many locals who attended the event. According to the World BOSAI Forum [1], the WBF2023 was attended by 5,412 people over 3 days, including 1,335 pre-registrants from 40 countries and regions, on-site registrants, and EXPO participants. It included 30 oral sessions, 7 conferences, 50 poster sessions, 33 flash talk presentations, and 33 exhibition booths, where tangible solutions for disaster risk reduction were discussed through dialogue among international organizations, governments, academia, the private sector, NGOs, and citizens.
At the forum, Tohoku University hosted 4 sessions and co-hosted 10 sessions, which introduced the role of Tohoku University and the International Research Institute of Disaster Science as well as their contributions to the processes of recovery and reconstruction from the 2011 GEJET.
Finally, the forum came up with recommendations for a Midterm Review of the Implementation of the Sendai Framework 2015–2030 (MTR SF). In May 2023, the High-Level Meeting of the General Assembly on the MTR SF was held at the United Nations Headquarters in New York [2].
The guest editors are pleased to publish this special issue in the Journal of Disaster Research, which comprises six articles sharing the research advancements presented at the WBF2023. We hope that this special issue on the WBF2023 will contribute to the literature on disaster science and further advance disaster risk reduction.
References:
[1] World BOSAI Forum, “WBF2023 Report,” 2023. https://worldBOSAIforum.com/2023/en/report/ [Accessed May 18, 2024]	[2] UNDRR, “High-Level Meeting on the Midterm Review of the Sendai Framework,” 2023. https://sendaiframework-mtr.undrr.org/high-level-meeting-midterm-review-sendai-framework-disaster-risk-reduction-2015-2030 [Accessed May 18, 2024]
Detailed protocol for the generation of polarized cortical assembloids (polCA)
Authors: Bosone Camilla , Elisabetta Barilà Sara , Krenn Veronica , Knoblich Jürgen A. 
Published: May 2024

Abstract

Cortical organoids capable of generating the major cortical cell types in distinct compartments are widely used to mechanistically study cortical development, evolution and related disorders. However, the lack of morphogen gradients imparting cortical positional information and topography within individual organoids hinders the solid investigation of complex phenotypes. Here, we describe a co-culture protocol of an organizer-like structure expressing the morphogen FGF8 with an organoid with an elongated shape, to generate a polarized source of FGF8 in individual organoids, referred to as polarized cortical assembloids (polCA). This protocol allows for the establishment of FGF8-dependent positional information along the longitudinal axis of individual organoids and spatially oriented populations with early transcriptional signatures relevant for a cortical protomap. This assembloid protocol can be used to combine organizer-like structures expressing various morphogens to allow for a reliable analysis of morphogens action on cortical positional identity. This protocol accompanies Bosone et al. 2024.
The minimal geoengineering problem concerning the Paris 2015 agreement
Authors: Bodai Tamas , Lembo Valerio , Aneesh Sundaresan , Lee Sun-Seon , Ishuzu Miho , Franz Matthias O. 
Published: May 2024

Abstract

There is a palpable shift in mainstream attitude towards geoengineering technologies, seen now as potential parts of a climate policy mix. Still, concerning solar radiation management (SRM) in particular, because of the known and unknown undesirable side-effects of various engineering implementations of theirs, it is important to know what is the minimal intervention that can achieve a certain goal. Such questions lead mathematically to inverse problems. Solving them is feasible only with lightweight models of the climate system, various types of which are nowadays often referred to as emulators – some more accurate than others. Here we develop an emulator using linear and nonlinear response theory and apply it to the minimal SRM problem concerning the Paris 2015 climate agreement, say, with the aim of constraining the global mean surface temperature below a certain limit. Our results suggest that SRM geoengineering, most commonly envisaged as sulfate aerosol injection, will likely have to be part of our climate policy mix, because realistic CO2 abatement effort to come alone cannot restrict global temperatures below the coveted 1.5 °C change or below even higher levels of change. Minimal sulfate use for the 1.5 °C limit is very likely to dictate immediate and rather abrupt deployment. However, SRM would be no use to achieve such a goal if the geoengineering- free “asymptotic” temperature is not below the target limit, as it would then need – in the absence of CDR – maintaining SRM “indefinitely”. The latter could be the case even if the temperature response to an anthropogenic CO2 emission pulse is nonmonotonic, and it would be certainly the case if it is monotonic. We show that the model that we use is near the boundary in parameter space between monotonic and nonmonotoninc temperature responses. In the unfortunate case of monotonicity concerning the real Earth system, the only use of SRM would be “buying time” to develop CDR.
Multistep allelic conversion in mouse pre-implantation embryos by AAV vectors
Authors: Nickl Petr , Jenickova Irena , Elias Jan , Kasparek Petr , Kopkanova Jana , Barinka Cyril , Sedlacek Radislav 
Published: May 2024

Abstract

Site-specific recombinases (SSRs) are critical for achieving precise spatiotemporal control of engineered alleles. These enzymes play a key role in facilitating the deletion or inversion of loci flanked by recombination sites, resulting in the activation or repression of endogenous genes, selection markers or reporter elements. However, multiple recombination in complex alleles can be laborious. To improve this, a new and efficient method using AAV vectors can simplify the conversion of systems based on Cre, Flpo, Dre and Vika recombinases. In this study, we present an effective method for ex vivo allele conversion using Cre, Flp (flippase), Dre, and Vika recombinases, employing adeno-associated viruses (AAV) as a delivery vector. AAVs enable efficient allele conversion with minimal toxicity in a reporter mouse line. Moreover, AAVs facilitate sequential allele conversion, essential for fully converting alleles with multiple recombination sites, typically found in conditional knockout mouse models. While simple allele conversions show a 100% efficiency rate, complex multiple conversions consistently achieve an 80% conversion rate. Overall, this strategy markedly reduces the need for animals and significantly speeds up the process of allele conversions, representing a significant improvement in genome engineering techniques.
Synthesis and Preclinical Evaluation of Novel 68Ga-Labeled Bispecific SSTR/GLP-1R Targeting Probes for Neuroendocrine Neoplasms Imaging
Authors: Ruan Chuyin , Yang Xiaoqiang , Qi Yongshuai , Chi Xiaohua , Li Guiping , Jiang Ying 
Published: May 2024

Abstract

Purpose
The neuroendocrine neoplasms (NENs) have a poor early diagnostic rate. To increase the detection rate of NENs, this project aims to build a PET probe, DOTA-exendin-4-TOC, that targets both SSTR and GLP-1R.
Procedures
The novel dual-target molecular probe DOTA-exendin-4-TOC was constructed, and radiolabeled with gallium-68 to target both SSTR and GLP-1R. In vivo and in vitro stability tests, cellular uptake tests, biodistribution, and microPET/CT studies were used to examine the PET probe properties in the tumor models including RIN-M5F, INS-1, and AR42J.
Results
The synthesis of [68Ga]Ga-DOTA-exendin-4-TOC had a radiochemical purity of more than 95%. The radiolabel demonstrated better stability both in vivo (in mouse serum) and in vitro (in PBS), and it is eliminated by the urine system. RIN-M5F cells, INS-1 cells, and AR42J cells were found to have an uptake effect on [68Ga]Ga-DOTA-exendin-4-TOC in vitro cell uptake experiments. The tumor models containing RIN-M5F, INS-1, and AR42J demonstrated uptake of [68Ga]Ga-DOTA-exendin-4-TOC at 60 and 120 minutes, according to microPET/CT imaging. Higher values of tumor uptake were noted for AR42J at 1.50 ± 0.10 (120 min), INS-1 at 1.57 ± 0.12 (60 min), and RIN-M5F at 0.87 ± 0.11 (60 min). All three tumor models exhibited tumor tissues' uptake of [68Ga]Ga-DOTA-exendin-4-TOC, according to in vivo metabolism studies. Furthermore, the kidneys had the highest distribution of radioactivity.
Conclusions
In this work, a new molecular probe that targets SSTR and GLP-1R was successfully constructed and radiolabeled with [68Ga]Ga, called [68Ga]Ga-DOTA-exendin-4-TOC. This novel dual-target probe could be applied as a valuable tool for improving the detection rate in patients in the future.
Sustainable Extraction Strategy for Lignin from Coconut Coir using Organosolv and Deep Eutectic Solvents (DES)
Authors: Agrawal Divykriti , Tripathi Anamika , Pal Pradipta , Hoque Moinal , Bharathi Sundaram Deepika , Jacob Samuel 
Published: May 2024

Abstract

Purpose
Lignocellulosic biomass serves as a potential source for the production of biomaterials and chemicals. Hence, this study focuses on utilizing a mild chemical route for lignin extraction to retain its native state thereby enhancing sustainability and cost-effectiveness.
Methods
Coconut coir, the selected biomass for lignin extraction is subjected to organosolv and Deep Eutectic Solvent (DES) treatments. Under the organosolv treatment strategy, two solvent-catalyst combinations were employed viz., ethanol and diethylene glycol (50%, v/v) along with FeCl3 and NaOH (3%, w/v) as a catalyst. Whereas, the DES treatments involve Choline Chloride (ChCl) and lactic acid (1:2), ChCl and urea (1:2) and ChCl and oxalic acid (1:1).
Results
During organosolv treatment, the maximum delignification was found to be 71.03 ± 1.90% (w/w) in ethanol + FeCl3 reflux, while DES treatment resulted in a maximum delignification of 60.93 ± 5.30% (w/w) with ChCl and urea at 120°C ± 2. The recovered lignin FTIR analysis revealed characteristic peaks comparable with the standard pure alkali/kraft lignin profile and the functional groups contained in lignin are correlated and indicated in the NMR profile as well.
Conclusion
The chosen treatments, show notable delignification while maintaining the native structure of the lignin, hence reinforcing the viability of lignin extraction sustainably and economically.
Optimization of the 5ʹ untranslated region of mRNA vaccines
Authors: 马 琦 , Zhang Xiaoguang , Yang Jing , Li Hongxia , Hao Yanzhe , Feng Xia 
Published: May 2024

Abstract

To investigate the impact of different 5ʹ untranslated regions (UTRs) on mRNA vaccine translation efficiency, five dual-reporter gene expression plasmids with different 5ʹUTRs were constructed. The corresponding mRNA transcripts were transcribed and capped in vitro. By comparing the expression levels of reporter genes with different 5'UTRs, we identified the 5'UTR associated with the highest expression level. Subsequently, HIVgp145 mRNA vaccines containing various 5'UTRs were constructed and verified. The results demonstrated that mRNA 3 (β-globin 5'UTR) displayed the greatest number of green fluorescence-positive cells and the highest luciferase fluorescence intensity in the reporter gene expression system. Further, among the HIVgp145 mRNA vaccines with different 5'UTRs, mRNA 7 (β-globin 5'UTR) exhibited the highest level of expression. These findings indicate that it is feasible to use the 5ʹUTR of β-globin in an mRNA vaccine, laying the foundation for animal immunogenicity testing.