bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deciphering novel mitochondrial signatures: multi-omics analysis uncovers cross-disease markers and oligodendrocyte pathways in Alzheimer’s disease and glioblastoma
Authors: Xu Xuan , Wang Jiaqi , Chen Tong , Wang Shuaibin , Wang Fei , He Junwen , Meng Xiang-Yu , Shen Yin 
Published: February 2025

IntroductionAlzheimer’s disease (AD) and glioblastoma (GBM) are severe neurological disorders that pose significant global healthcare challenges. Despite extensive research, the molecular mechanisms, particularly those involving mitochondrial dysfunction, remain poorly understood. A major limitation in current studies is the lack of cell-specific markers that effectively represent mitochondrial dynamics in AD and GBM.MethodsIn this study, we analyzed single-cell transcriptomic data using 10 machine learning algorithms to identify mitochondria-associated cell-specific markers. We validated these markers through the integration of gene expression and methylation data across diverse cell types. Our dataset comprised single-nucleus RNA sequencing (snRNA-seq) from AD patients, single-cell RNA sequencing (scRNA-seq) from GBM patients, and additional DNA methylation and transcriptomic data from the ROSMAP, ADNI, TCGA, and CGGA cohorts.ResultsOur analysis identified four significant cross-disease mitochondrial markers: EFHD1, SASH1, FAM110B, and SLC25A18. These markers showed both shared and unique expression profiles in AD and GBM, suggesting a common mitochondrial mechanism contributing to both diseases. Additionally, oligodendrocytes and their interactions with astrocytes were implicated in disease progression, particularly through the APP signaling pathway. Key hub genes, such as HS6ST3 and TUBB2B, were identified across different cellular subpopulations, highlighting a cell-specific co-expression network linked to mitochondrial function.
Research progress on brain network imaging biomarkers of subjective cognitive decline
Authors: Yingmei Han , Chaojie Wang , Yi Zhang , Yijie Li , Heng Zhang , Ze Feng , Weiqing Li , Bingyuan Chu , Feng Wang 
Published: February 2025

PurposeSubjective cognitive decline (SCD) is an early manifestation of the Alzheimer’s disease (AD) continuum, and accurately diagnosing SCD to differentiate it from neurotypical aging in older adults is a common challenge for researchers.MethodsThis review examines and summarizes relevant studies regarding the neuroimaging of the AD continuum, and comprehensively summarizes and outlines the SCD clinical features characterizing along with the corresponding neuroimaging changes involving structural, functional, and metabolic networks.ResultsThe clinical characteristics of SCD include a subjective decline in self-perceived cognitive function, and there are significant imaging changes, such as reductions in gray matter volume in certain brain regions, abnormalities in the integrity of white matter tracts and diffusion metrics, alterations in functional connectivity between different sub-networks or within networks, as well as abnormalities in brain metabolic networks and cerebral blood flow perfusion.ConclusionThe 147 referenced studies in this paper indicate that exploring the structural, functional, and metabolic network changes in the brain related to SCD through neuroimaging aims to enhance the goals and mission of brain science development programs: “Understanding the Brain,” “Protecting the Brain,” and “Creating the Brain,” thereby strengthening researchers’ investigation into the mechanisms of brain function. Early diagnosis of SCD, along with prompt intervention, can reduce the incidence of AD spectrum while improving patients’ quality of life, even integrating numerous scientific research achievements into unified and established standards and applying them in clinical practice by doctors, thus all encouraging researchers to further investigate SCD issues in older adults.
The human 18S rRNA m6A methyltransferase METTL5 promotes tumorigenesis via DEPDC1 in lung squamous cell carcinoma
Authors: Yan Yang , Fu Jianjun 
Published: February 2025

BackgroundN6-Methyladenosine (m6A) is one of the post-transcriptional modifications and abnormal m6A is critical for cancer initiation, progression, metastasis in Lung squamous cell carcinoma (LUSC). Ribosomal RNA (rRNA) accounts for most of the total cellular RNA, however, the functions and molecular mechanisms underlying rRNA modifications in LUSC remained largely unclear.MethodsHigh-throughput library screening identifies the key m6A regulator METTL5 in LUSC. Cell and animal experiments were used to identify that METTL5 promoted LUSC tumorigenesis to enhance DEP domain containing 1 (DEPDC1) translation via m6A modification.ResultsWe showed that the N6-methyladenosine (m6A) methyltransferase METTL5 was an independent risk factor in LUSC and was associated with poor prognosis of patients. Notedly, overexpression METTL5 promoted LUSC tumorigenesis in an m6A modification, while METTL5 knockdown markedly inhibited proliferation and migratory ability of tumor cells in vitro and in vivo. Mechanistically, METTL5 promoted LUSC tumorigenesis via m6a methyltransferase to increase the translation of DEPDC1.ConclusionOur results revealed that METTL5 enhances DEPDC1 translation to contribute to tumorigenesis and poor prognosis, providing a potential prognostic biomarker and therapeutic target for LUSC.
Tofacitinib treatment of systemic juvenile idiopathic arthritis: a case report and literature review
Authors: Zhu Meifang , Zhao Yan , Zhang Xiaohua , Zhou Peng , Jin Jing , Fan Zhidan , Yu Haiguo 
Published: February 2025

ObjectiveSystemic juvenile idiopathic arthritis (sJIA), a particularly aggressive form of childhood arthritis, is characterized by persistent systemic inflammation. The most advanced treatments include biologic agents that target the interleukin-1(IL-1) and interleukin-6(IL-6) pathways. However, sJIA continue to pose challenging challenges for rheumatologists treating pediatric patients worldwide.Methods1 children with sJIA was retrospectively collected from the Department of Rheumatology and immunology, Children's Hospital of Nanjing Medical University, Nanjing. Literature published between 2019 and 2024 was reviewed to understand the effect of tofacitinib on patients with sJIA.ResultsAfter a month of treatment of tofacitinib, there was a significant improvement in clinical symptoms and inflammatory indicators showed a marked decrease. As of July 2023, the patient's condition was effectively in remission. The efficacy of tofacitinib treatment was remarkable.ConclusionTofacitinib has shown good efficacy and safety in the treatment of sJIA patients, effectively controlling disease activity and relieving symptoms. The application of Janus kinase (JAK) inhibitors may offer a new treatment option for this disease.
Preterm-born children, screen time, and spectacle wear in the Longitudinal Preterm Outcome Project: a cohort study
Authors: Iyer V. , de Kroon M. L. A. , Klaver C. C. W. , Reijneveld S. A. 
Published: February 2025

IntroductionPreterm born children are at a higher risk for refractive errors. A long duration of screen time and activities with short working distance (≤30 cm) may further add to the increased risk. The aim of this study was to assess the separate and combined effects of preterm birth and screen time on spectacle wear among 5-year-olds and adolescents.MethodsWe analyzed data from the community-based preterm cohort study, part of the Longitudinal Preterm Outcome Project (LOLLIPOP). Early preterm-born (EP &lt; 32 weeks), moderately-late preterm-born (MLP 32–36 weeks) and full-term born (FT 38–42 weeks) children were followed. Spectacle wear and screen time were assessed by questionnaire at the age of 5 (n = 1,515) and at adolescence, ages 13–16, for a subsample (n = 227).ResultsAt age 5, the prevalences of spectacle wear were 7.8%, 7.6% and 3.2%, for EP, MLP, and FT children, respectively (p = 0.007); the risk of spectacle wear decreased by 7% for each additional week of gestational age. In adolescence, prevalences were 36.6%, 20.8% and 22.4%, for EP, MLP, and FT children, respectively (p = 0.12). We found no relationship between screen-time and spectacle wear or a combined effect with preterm birth at age 5 or adolescence.ConclusionsEP and MLP children have a significantly increased risk of spectacle wear at age 5, but not significantly at adolescence. At that age, the prevalences of spectacle wear were generally higher. We found no evidence for an association of screen time preterm birth with spectacle wear, and neither an impact of screentime on such an association.
Medroxyprogesterone promotes neuronal survival after cerebral ischemic stroke by inhibiting PARthanatos
Authors: Yue Chang-Ling , Ding Yan-Xia , Chen Meng , Shen Yu-Xin , Hu Ao-Meng , Huang Hai , Zhang Zhao-Huan , Zhou Ying-Xin , Xu Xiao-Hui 
Published: February 2025

IntroductionCerebral ischemic stroke (CIS) is caused by the interruption of cerebral blood circulation due to thrombosis or embolism and is the second-leading cause of mortality worldwide. The neuronal death and motor dysfunction resulting from CIS are primarily attributed to the induction of PARthanatos in neurons at the site of ischemia. Blocking parthanatos is a promising treatment for CIS.MethodsThe effect of medroxyprogesterone treatment on PARthanatos in vitro was examined by CCK8 assay and flow cytometry and the target protein of medroxyprogesterone was then identified by a series of assays, including western blotting, immunofluorescence, cell thermal shift assay and molecular docking. Subsequently, the efficacy of medroxyprogesterone in the treatment of ischemic stroke was evaluated by FJC staining.ResultsIn our study, medroxyprogesterone was able to block the occurrence of PARthanatos in Hela cells induced by MNNG. PARP-1 activity did not change after medroxyprogesterone treatment but prevented MNNG-induced apoptosis inducing factor (AIF) release from mitochondria by improving the stability of phosphorylated extracellular signal-regulated kinase (ERK). In vivo, medroxyprogesterone significantly reduces neuronal death in mouse models of CIS by inhibiting PARthanatos.ConclusionOur findings indicate that medroxyprogesterone effectively inhibits PARthanatos not by affecting the activity of PARP-1, but by directly binding to ERK and stabilizes the active phosphorylated ERK, thereby inhibiting AIF translocation. Furthermore, medroxyprogesterone shows potential as a neuroprotective agent for patients with CIS, potentially enhancing post-stroke recovery and reducing societal burdens.
Exploring skin adverse events and mechanisms of apalutamide using data mining algorithms and network pharmacology
Authors: Chen Yaqing , Huang Longzhuan , Li Wenwei , Gu Hangye , Chen Yong 
Published: February 2025

BackgroundSkin adverse events of apalutamide pose a major challenge to its clinical use, particularly the severe and difficult to identify toxic epidermal necrolysis. For the purpose of providing the basis for the clinical monitoring of the administration of apalutamide and further research. This study examined the pathways of apalutamide and Stephen Johnson Syndrome/Toxic Epidermal Necrolysis using network pharmacology and data mining tools to analyze skin adverse events.MethodsUsing the Information Component method and the Reporting Odds Ratio, the relationship between apalutamide and skin adverse events was evaluated. Molecular docking was utilized to explore the potential mechanism of apalutamide and Stephen Johnson Syndrome/toxic epidermal necrolysis.ResultsWith a median time to onset of all skin adverse events of 55 days, a total of 21 skin-related adverse events were found. Low body weight and advanced age may be major hazards for skin adverse events with apalutamide. The results showed a substantial association between apalutamide and Stephen Johnson Syndrome/toxic epidermal necrolysis, and the mechanism behind this association may be the binding of apalutamide to JAK1 and JAK2.ConclusionSpecial attention is recommended for skin adverse events when using apalutamide, especially for rapidly progressing and severe adverse events. To confirm the connection between the triad of Janus kinase, apalutamide, and skin adverse events, further research is required in the future.
Laboratory comparison of consumer-grade and research-established wearables for monitoring heart rate, body temperature, and physical acitivity in sub-Saharan Africa
Authors: Mendt Stefan , Zout Georgi , Rabuffetti Marco , Gunga Hanns-Christian , Bunker Aditi , Barteit Sandra , Maggioni Martina Anna 
Published: February 2025

BackgroundConsumer-grade wearables are becoming increasingly popular in research and in clinical contexts. These technologies hold significant promise for advancing digital medicine, particularly in remote and rural areas in low-income settings like sub-Saharan Africa, where climate change is exacerbating health risks. This study evaluates the data agreement between consumer-grade and research-established devices under standardized conditions.MethodsTwenty-two participants (11 women, 11 men) performed a structured protocol, consisting of six different activity phases (sitting, standing, and the first four stages of the classic Bruce treadmill test). We collected heart rate, (core) body temperature, step count, and energy expenditure. Each variable was simultaneously tracked by consumer-grade and established research-grade devices to evaluate the validity of the consumer-grade devices. We statistically compared the data agreement using Pearson’s correlation r, Lin’s concordance correlation coefficient (LCCC), Bland-Altman method, and mean absolute percentage error.ResultsA good agreement was found between the wrist-worn Withings Pulse HR (consumer-grade) and the chest-worn Faros Bittium 180 in measuring heart rate while sitting, standing, and slow walking on a treadmill at a speed of 2.7 km/h (r ≥ 0.82, |bias| ≤ 3.1 bpm), but this decreased with increasing speed (r ≤ 0.33, |bias| ≤ 11.7 bpm). The agreement between the Withing device and the research-established device worn on the wrist (GENEActiv) for measuring the number of steps also decreased during the treadmill phases (first stage: r = 0.48, bias = 0.6 steps/min; fourth stage: r = 0.48, bias = 17.3 steps/min). Energy expenditure agreement between the Withings device and the indirect calorimetry method was poor during the treadmill test (|r| ≤ 0.29, |bias | ≥ 1.7 MET). The Tucky thermometer under the armpit (consumer-grade) and the Tcore sensor on the forehead were found to be in poor agreement in measuring (core) body temperature during resting phases (r ≤ 0.53, |bias| ≥ 0.8°C) and deteriorated during the treadmill test.ConclusionThe Withings device showed adequate performance for heart rate at low activity levels and step count at higher activity levels, but had limited overall accuracy. The Tucky device showed poor agreement with the Tcore in all six different activity phases. The limited accuracy of consumer-grade devices suggests caution in their use for rigorous research, but points to their potential utility in capture general physiological trends in long-term field monitoring or population-health surveillance.
Electrocortical signatures of attentional bias toward subliminal and supraliminal socially negative words in social anxiety
Authors: Gan Shuzhen , Cai Yanglong , Li Weijun 
Published: February 2025

BackgroundPrevious research has demonstrated that abnormal attentional bias toward social threats at different processing stages is pivotal for the development and maintenance of social anxiety. However, the temporal property and the neural indicators of this bias are still open to clarification. The present study employed event-related potential (ERP) methodology to investigate the attentional bias toward social threats at the early preconscious and later controlled processing stages, along with associated electrocortical indicators.MethodsSocially or non-socially negative words paired with neutral ones were presented subliminally and supraliminally in two dot-probe tasks, respectively. Twenty-six participants with high level of social anxiety (high SA) and twenty-four participants with low level of social anxiety (low SA) completed the tasks.ResultsThe results revealed that, compared to the low SA group, the high SA group specifically showed a significant N2pc in response to subliminal socially negative words, and the amplitude tended to correlate with anxious severity. Additionally, the high SA group exhibited greater amplitudes of parietal P3 in response to incongruent probes than congruent ones following both subliminal and supraliminal socially negative words.ConclusionThese results indicate that abnormal attentional bias of social anxiety includes both early preconscious attentional orienting to social threats and subsequent difficulty disengaging from conscious and unconscious social threats, as indexed by N2pc and parietal P3 components, respectively. Our study may hold clinical significance by providing electrophysiological markers for assessing the cognitive symptoms of social anxiety.
Association between depressive status and mild cognitive impairment in middle-aged and elderly Chinese adults from CHARLS study
Authors: Wei Caijuan , Zhao Jinyu , Hu Rui , Wei Xingli 
Published: February 2025

BackgroundThe potential association between depressive status and the risk of mild cognitive impairment (MCI) remains unclear, especially in the absence of prospective evidence. This study aims to elucidate the impact of either depression score or depression on the risk of MCI using longitudinal data from the China Health and Retirement Longitudinal Study (CHARLS).MethodsThis prospective study included 5,766 participants from CHARLS followed from 2011 to 2015. We calculated the baseline depression score using the 10-item Center for Epidemiologic Studies Depression Scale (CESD-10) and the cognitive status score after 3 years of follow-up through four dimensions: orientation, memory, calculation, and draw. We collected baseline sociological characteristics and health-related factors as covariates, using multivariate-adjusted logistics regression models (odds ratios (OR) and 95% confidence intervals (CI)) and restricted cubic splines (RCS) to estimate the effect of depressive status on MCI risk.ResultsWe observed 724 new cases of MCI at follow-up. Logistics regression analysis showed that participants with depression had a 58% higher risk of developing MCI than those without depression (OR = 1.58, 95%CI: 1.35-1.85), and the positive association persisted after adjusting for covariates such as sociological characteristics of the population and health-related factors (OR = 1.24, 95%CI: 1.04-1.48). We also observed a dose-response relationship between depression score and MCI risk, with participants with 11~20 and 21~30 scores having a progressively higher risk of MCI compared to participants with depression score of 0~10 (p for trend &lt; 0.05), and a 3% increase in MCI risk for each 1-point increase in depression scores (OR = 1.03, 95%CI: 1.01-1.04). RCS analysis also showed a nonlinear association between depression score and MCI risk (p for non-linearity = 0.001), with MCI risk increasing with increasing depression score. In addition, stratified analyses based on sex, age, marital status, residence, BMI, nighttime sleep, smoking status, alcohol drinking status, baseline serological indicators, and comorbidities showed no interaction (p for interaction &gt; 0.05) other than serum total cholesterol levels (p for interaction = 0.008).ConclusionsAmong middle-aged and elderly adults from CHARLS, depression is an independent risk factor for MCI, indicating that individuals with more severe depression symptoms are more likely to suffer from MCI. Early depression screening based on CESD-10 may help identify individuals at high risk of MCI, and early intervention may reduce the incidence of MCI and Alzheimer’s disease, thereby reducing the social care burden of an ageing population.
Knowledge, attitudes, behaviors, and information needs of women vaccinated with the HPV vaccine regarding cervical cancer prevention: a cross-sectional study
Authors: Zhou Xuan , Wu Miaomiao , Zhou Yuling , Su Fang , He Yiqing , Ding Jinxia , Xie Lunfang 
Published: February 2025

BackgroundCervical cancer poses a serious threat to women’s health globally, especially in China. HPV vaccination and screening are crucial prevention and control measures. However, the screening coverage among Chinese women remains low, and there is a need to better understand the knowledge, attitudes, behaviors, and information needs of Chinese HPV-vaccinated women regarding cervical cancer prevention to optimize prevention and control strategies.ObjectivesTo explore the knowledge, attitudes, behaviors, and information needs of women vaccinated with the HPV vaccine regarding cervical cancer prevention.MethodsThis cross-sectional study was conducted using a convenience sampling method from October 1 to December 30, 2023. A questionnaire survey was administered to 439 women vaccinated with the HPV vaccine at the Shu Shan District Community Health Service Center in Hefei, Anhui Province. The survey tool was self-designed. Data were analyzed using descriptive statistics, chi-square tests, and binary logistic regression.ResultsThe average age of the 439 participants was 27.82 ± 6.42 years. The average cervical cancer prevention knowledge score was 35.01 ± 5.76. 434 (98.9%) women held a positive attitude towards cervical cancer screening, and 320 (72.9%) women had undergone cervical cancer screening after receiving the HPV vaccine. Educational levels such as college (OR = 2.995, 95%CI: 1.233–7.279, p = 0.015), bachelor’s degree (OR = 3.694, 95%CI: 1.718–7.943, p = 0.001), and postgraduate and above (OR = 4.826, 95%CI: 2.176–10.707, p &lt; 0.001), as well as occupation as medical workers (OR = 4.660, 95%CI: 2.292–9.474, p &lt; 0.001), were associated with higher knowledge of prevention and treatment scores. Individuals aged 26–35 years (OR = 7.431, 95%CI: 2.856–19.331, p &lt; 0.001), 36–45 years (OR = 11.466, 95%CI: 2.279–57.694, p = 0.003), married individuals (OR = 4.307, 95%CI: 1.455–12.750, p = 0.008), and participants who had received health education related to cervical cancer prevention (OR = 2.125, 95%CI: 1.169–3.863, p = 0.013) and possessed good knowledge of prevention (OR = 16.770, 95%CI: 8.667–32.451, p &lt; 0.001) were more inclined to undergo cervical cancer screening. Among the 254 participants who had received health education, 34.2% still had unmet information needs regarding cervical cancer prevention, and 29.5% hoped to receive health education services from professionals.ConclusionChinese HPV-vaccinated women have a good understanding of cervical cancer prevention and a positive attitude and behavior towards cervical cancer screening. However, their knowledge of cervical cancer screening is not sufficient, and their information needs have not been fully met.