bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recurrent retroperitoneal Schwannomas displaying different differentiation from primary tumor: Case report and literature review
Authors: Li Zhong-qi , Wang Hai-yong , Li Jun , Teng Lisong 
Published: August 2010

Abstract

Background
Retroperitoneal Schwannomas are uncommonly found in the retroperitoneum and few of them show malignant transformation and invasion. Local recurrence are common in malignant Schwannomas with very few reports of tumor distinct differentiation at recurrences.

Case presentation
We report here a rare case of retroperitoneal schwannoma with multiple origins from retroperitoneum and pelvic wall. Pathological examination confirmed the case as a schwannoma with malignant transformation. Radical dissection of the tumors along with the sacrifice of adjacent sigmoid colon and left kidney failed to provide a cure for this patient. Due to tumor recurrence, a second and a third surgery of radical excision were performed 6 months and 17 months later after the first surgery, respectively. Histopathologic analysis identified that the recurrent tumors were different from the original schwannoma because of their smooth muscle-like differentiation.

Conclusion
Malignant schwannomas are uncommon sarcomas with a high incidence of local recurrence. Distinct immunohistochemical staining results of the tumors at recurrence indicate their potential of smooth-muscle like differentiation. Radical excision of the tumors may provide benefit for their local recurrences.
Secondary infection with Streptococcus suis serotype 7 increases the virulence of highly pathogenic porcine reproductive and respiratory syndrome virus in pigs
Authors: Xu Min , Wang Shujie , Li Linxi , Lei Liancheng , Liu Yonggang , Shi Wenda , Wu Jiabin , Li Liqin , Rong Fulong , Xu Mingming , Sun Guangli , Xiang Hua , Cai Xuehui 
Published: August 2010

Abstract

Background
Porcine reproductive and respiratory syndrome virus (PRRSV) and Streptococcus suis are common pathogens in pigs. In samples collected during the porcine high fever syndrome (PHFS) outbreak in many parts of China, PRRSV and S. suis serotype 7 (SS7) have always been isolated together. To determine whether PRRSV-SS7 coinfection was the cause of the PHFS outbreak, we evaluated the pathogenicity of PRRSV and/or SS7 in a pig model of single and mixed infection.

Results
Respiratory disease, diarrhea, and anorexia were observed in all infected pigs. Signs of central nervous system (CNS) disease were observed in the highly pathogenic PRRSV (HP-PRRSV)-infected pigs (4/12) and the coinfected pigs (8/10); however, the symptoms of the coinfected pigs were clearly more severe than those of the HP-PRRSV-infected pigs. The mortality rate was significantly higher in the coinfected pigs (8/10) than in the HP-PRRSV- (2/12) and SS7-infected pigs (0/10). The deceased pigs of the coinfected group had symptoms typical of PHFS, such as high fever, anorexia, and red coloration of the ears and the body. The isolation rates of HP-PRRSV and SS7 were higher and the lesion severity was greater in the coinfected pigs than in monoinfected pigs.

Conclusion
HP-PRRSV infection increased susceptibility to SS7 infection, and coinfection of HP-PRRSV with SS7 significantly increased the pathogenicity of SS7 to pigs.
Sarcoidosis in a dental surgeon: a case report
Authors: Checchi Luigi , Nucci Maria C , Gatti Antonietta M , Mattia Daniele , Violante Francesco S 
Published: August 2010

Abstract

Introduction
Although the causes of sarcoidosis are still unknown, past and current studies have provided evidence that this disease may be associated with occupational exposure to specific environmental agents. We describe a case of sarcoidosis in a dental surgeon with long exposure to inorganic dusts. To the best of our knowledge, this is the first report of this kind in the literature.

Case presentation
At the beginning of 2000, a 52-year-old Caucasian man, who worked as a dental surgeon, presented with shortness of breath during exercise, cough and retrosternal pain. After diagnosis of sarcoidosis, a scanning electronic microscopy with X-ray microanalysis of biopsy specimens was used in order to determine whether the disease could be traced to an occupational environmental agent. Results showed the presence of inorganic dust particles within sarcoidotic granulomas, and demonstrated that the material detected was identical to that found in a powder used by our patient for several years.

Conclusions
Although these results cannot be considered as definitive proof, they do however provide strong evidence that this disease may be associated with material used by dental surgeons.
Achievements and challenges in bioartificial kidney development
Authors: Tasnim Farah , Deng Rensheng , Hu Min , Liour Sean , Li Yao , Ni Ming , Ying Jackie Y , Zink Daniele 
Published: August 2010

AbstractBioartificial kidneys (BAKs) combine a conventional hemofilter in series with a bioreactor unit containing renal epithelial cells. The epithelial cells derived from the renal tubule should provide transport, metabolic, endocrinologic and immunomodulatory functions. Currently, primary human renal proximal tubule cells are most relevant for clinical applications. However, the use of human primary cells is associated with many obstacles, and the development of alternatives and an unlimited cell source is one of the most urgent challenges. BAKs have been applied in Phase I/II and Phase II clinical trials for the treatment of critically ill patients with acute renal failure. Significant effects on cytokine concentrations and long-term survival were observed. A subsequent Phase IIb clinical trial was discontinued after an interim analysis, and these results showed that further intense research on BAK-based therapies for acute renal failure was required. Development of BAK-based therapies for the treatment of patients suffering from end-stage renal disease is even more challenging, and related problems and research approaches are discussed herein, along with the development of mobile, portable, wearable and implantable devices.
Transactivation of PDGFRβ by dopamine D4 receptor does not require PDGFRβ dimerization
Authors: Chi Sum Shing , Vetiska Sandra M , Gill Robin S , Hsiung Marilyn S , Liu Fang , Van Tol Hubert HM 
Published: August 2010

Abstract
Growth factor-induced receptor dimerization and cross-phosphorylation are hallmarks of signal transduction via receptor tyrosine kinases (RTKs). G protein-coupled receptors (GPCRs) can activate RTKs through a process known as transactivation. The prototypical model of RTK transactivation involves ligand-mediated RTK dimerization and cross-phosphorylation. Here, we show that the platelet-derived growth factor receptor β (PDGFRβ) transactivation by the dopamine receptor D4 (DRD4) is not dependent on ligands for PDGFRβ. Furthermore, when PDGFRβ dimerization is inhibited and receptor phosphorylation is suppressed to near basal levels, the receptor maintains its ability to be transactivated and is still effective in signaling to ERK1/2. Hence, the DRD4-PDGFRβ-ERK1/2 pathway can occur independently of a PDGF-like ligand, PDGFRβ cross-phosphorylation and dimerization, which is distinct from other known forms of transactivation of RTKs by GPCRs.
Parthenolide induces proliferation inhibition and apoptosis of pancreatic cancer cells in vitro
Authors: Liu Jun-Wei , Cai Min-Xia , Xin Ying , Wu Qing-Song , Ma Jun , Yang Po , Xie Hai-Yang , Huang Dong-Sheng 
Published: August 2010

Abstract

Background
To explore the anti-tumor effects of parthenolide in human pancreatic cancer.

Methods
BxPC-3 cell, a human pancreatic cancer, was treated with parthenolide at different concentrations. The MTT assay was used to analyze cell viability. Flow cytometry and DNA fragmentation analysis were applied to evaluate apoptosis after parthenolide treatment. The wound closure and cell invasion assay were also employed in the study. Western blotting was used to demonstrate Bad, Bcl-2, Bax, caspase-9 and pro-caspase-3 expression.

Results
The MTT assay indicated that the pancreatic cancer growth could be dose-dependently inhibited by parthenoolide. This phenomenon was confirmed by flow cytometry and DNA fragmentation analysis. The wound closure assay and cell invasion assay showed that BxPC-3 cell was significantly suppressed by parthenolide at 7.5 μM and 15 μM. Western Blotting demonstrated the Bcl-2 and pro-caspase-3 were down-regulated while the Bax and caspase-9 were up-regulated. No alteration in Bad expression was found after treatment.

Conclusions
The parthenolide can inhibit the cell growth, migration, and induce the apoptosis in human pancreatic cancer. These findings may provide a novel approach for pancreatic cancer treatment.
Bone events and evolution of biologic markers in Gaucher disease before and during treatment
Authors: Stirnemann Jérôme , Belmatoug Nadia , Vincent Corine , Fain Olivier , Fantin Bruno , Mentré France 
Published: August 2010

Abstract
Introduction
Known biomarkers of Gaucher-disease activity are platelets, chitotriosidase, angiotensin-converting enzyme (ACE), tartrate-resistant acid phosphatase (TRAP) and ferritin. The aim of this study was to retrospectively evaluate the frequency of bone events (BE) and biomarker changes during two periods: diagnosis to first enzyme-replacement therapy (ERT) and the latter to the closing date.

Methods
BE of 62 treated patients, among the 73-patient cohort followed at Beaujon Hospital, Clichy, France, were described with Kaplan-Meier curves, and linear-mixed models were used to analyze their biomarker changes and the influence of several covariates (splenectomy, diagnosis year, genotype, age at diagnosis and sex).

Results
BE occurred before (54 events in 21 patients), but also during, ERT (12 events in 10 patients), with respective frequencies (95% confidence interval) at 10 years of 22.4% (13.3 to 36.3) and 20.0% (10.2 to 36.9). Biomarker slope changes before and during ERT differed significantly for platelets (+190/mm3/year and 7,035/mm3/year, respectively; P < 0.0001) and ferritin (+4% and -14%; P < 0.0001). High ferritin levels and low platelet counts at ERT onset were significantly associated with BE during ERT (P = 0.019 and 0.039, respectively). Covariates significantly influenced biomarker changes (baseline and/or slope): splenectomy affected platelets (baseline and changes), TRAP changes and chitotriosidase changes; diagnosis date influenced ACE and TRAP baseline values; and genotype influenced chitotriosidase baseline and changes.

Conclusions
Platelet counts and ferritin levels and their slope changes at ERT onset seem to predict BE during treatment. Biomarker baseline values and changes are dependent on several covariables.
TP53 Mutation and Survival in Chronic Lymphocytic Leukemia
Authors: Zenz Thorsten , Eichhorst Barbara , Busch Raymonde , Denzel Tina , Häbe Sonja , Winkler Dirk , Bühler Andreas , Edelmann Jennifer , Bergmann Manuela , Hopfinger Georg , Hensel Manfred , Hallek Michael , Döhner Hartmut , Stilgenbauer Stephan 
Published: August 2010

Purpose The precise prognostic impact of TP53 mutation and its incorporation into treatment algorithms in chronic lymphocytic leukemia (CLL) is unclear. We set out to define the impact of TP53 mutations in CLL. Patients and Methods We assessed TP53 mutations by denaturing high-performance liquid chromatography (exons 2 to 11) in a randomized prospective trial (n = 375) with a follow-up of 52.8 months (German CLL Study Group CLL4 trial; fludarabine [F] v F + cyclophosphamide [FC]). Results We found TP53 mutations in 8.5% of patients (28 of 328 patients). None of the patients with TP53 mutation showed a complete response. In patients with TP53 mutation, compared with patients without TP53 mutation, median progression-free survival (PFS; 23.3 v 62.2 months, respectively) and overall survival (OS; 29.2 v 84.6 months, respectively) were significantly decreased (both P < .001). TP53 mutations in the absence of 17p deletions were found in 4.5% of patients. PFS and OS for patients with 17p deletion and patients with TP53 mutation in the absence of 17p deletion were similar. Multivariate analysis identified TP53 mutation as the strongest prognostic marker regarding PFS (hazard ratio [HR] = 3.8; P < .001) and OS (HR = 7.2; P < .001). Other independent predictors of OS were IGHV mutation status (HR = 1.9), 11q deletion (HR = 1.9), 17p deletion (HR = 2.3), and FC treatment arm (HR = 0.6). Conclusion CLL with TP53 mutation carries a poor prognosis regardless of the presence of 17p deletion when treated with F-based chemotherapy. Thus, TP53 mutation analysis should be incorporated into the evaluation of patients with CLL before treatment initiation. Patients with TP53 mutation should be considered for alternative treatment approaches.
Consolidation Therapy With Subcutaneous Alemtuzumab After Fludarabine and Rituximab Induction Therapy for Previously Untreated Chronic Lymphocytic Leukemia: Final Analysis of CALGB 10101
Authors: Lin Thomas S. , Donohue Kathleen A. , Byrd John C. , Lucas Margaret S. , Hoke Eva E. , Bengtson Elizabeth M. , Rai Kanti R. , Atkins James N. , Link Brian K. , Larson Richard A. 
Published: August 2010

Purpose To determine if alemtuzumab consolidation improves response rate and progression-free survival (PFS) after induction chemoimmunotherapy in previously untreated symptomatic patients with chronic lymphocytic leukemia. Patients and Methods Patients (n = 102) received fludarabine 25 mg/m2 intravenously days 1 to 5 and rituximab 50 mg/m2 day 1, 325 mg/m2 day 3, and 375 mg/m2 day 5 of cycle 1 and then 375 mg/m2 day 1 of cycles 2 to 6; fludarabine plus rituximab (FR) administration was repeated every 28 days for six cycles. Three months after completion of FR, patients with stable disease or better response received subcutaneous alemtuzumab 3 mg day 1, 10 mg day 3, and 30 mg day 5 and then 30 mg three times per week for 5 weeks. Results Overall response (OR), complete response (CR), and partial response (PR) rates were 90%, 29%, and 61% after FR, respectively; 15% of patients were minimal residual disease (MRD) negative. Of 102 patients, 58 received alemtuzumab; 28 (61%) of 46 patients achieving PR after FR attained CR after alemtuzumab. By intent to treat (n = 102), OR and CR rates were 90% and 57% after alemtuzumab, respectively; 42% of patients became MRD negative. With median follow-up of 36 months, median PFS was 36 months, 2-year PFS was 72%, and 2-year OS was 86%. In patients achieving CR after FR, alemtuzumab was associated with five deaths resulting from infection (viral and Listeria meningitis and Legionella, cytomegalovirus, and Pneumocystis pneumonias), which occurred up to 7 months after last therapy. The study was amended to exclude CR patients from receiving alemtuzumab. Conclusion Alemtuzumab consolidation improved CR and MRD-negative rates after FR induction but caused serious infections in patients who had already achieved CR after induction and did not improve 2-year PFS or survival.
Total absolute horospherical curvature of submanifolds in hyperbolic space
Authors: Buosi Marcelo , Izumiya Shyuichi , Soares Ruas Maria Aparecida 
Published: August 2010

AbstractWe study the horospherical geometry of submanifolds in hyperbolic space. The main result is a formula for the total absolute horospherical curvature ofM, which implies, for the horospherical geometry, the analogues of classical inequalities of the Euclidean Geometry. We prove the horospherical Chern–Lashof inequality for surfaces in 3-space and the horospherical Fenchel and Fary–Milnor's theorems.
Effective non-vanishing conjectures for projective threefolds
Authors: Broustet Amäel , Höring Andreas 
Published: August 2010

Abstract
Let X be a smooth projective threefold, and let A be an ample line bundle such that KX
+ A is nef. We show that if KX
or –KX
is pseudo-effective, the adjoint bundle KX
+ A has global sections. We also give a very short proof of the Beltrametti–Sommese conjecture in dimension three, recently proven by Fukuma: if A is an ample line bundle such that KX
+ 2A is nef, the adjoint bundle KX
+ 2A has global sections.
Addendum to the synoptic review of red algal genera
Authors: Wynne Michael J. , Schneider Craig W. 
Published: August 2010

Abstract
An addendum to Schneider and Wynne's A synoptic review of the classification of red algal genera a half century after Kylin's “Die Gattungen der Rhodophyceen” (2007; Bot. Mar.
50: 197–249) is presented, with an updating of names of new taxa at the generic level and higher. In the last few years, the names of several new orders and families of red algae have been validated; these are cited and referenced below.
New macroalgal records for the Aegean Sea (Greece, eastern Mediterranean Sea)
Authors: Tsiamis Konstantinos , Verlaque Marc , Panayotidis Panayotis , Montesanto Barbara 
Published: August 2010

Abstract
Nine macroalgal taxa are reported for the first time from Greece (Aegean Sea and nearby areas): the chlorophyte Cladophora nigrescens, the phaeophyceans Cystoseira susanensis and Dictyopteris lucida, and the rhodophytes Centroceras gasparrinii, Ophidocladus simpliciusculus, Peyssonnelia coriacea, Porphyrostromium ciliare, Radicilingua thysanorhizans and Rodriguezella ligulata. Peyssonnelia stoechas, a species recently reported from Greece (Karpathos Island), is described for the first time from the Gulf of Korinthiakos. Several of the species are rare in the Mediterranean Sea, and five are new to the eastern basin, thus extending their known distribution ranges. Our results indicate that the biodiversity of the eastern Mediterranean marine flora is probably richer than originally thought. A description, illustrations, habitat, geographic distribution and taxonomic remarks are provided for each taxon.
Use of Miniature Specimen Test and Finite Element Method for Evaluation of Yield Strength and Fracture Toughness in Metallic Alloys
Authors: Reddy K. Harshavardhan , Partheepan G. , Pandey R. K. , Sehgal D. K. 
Published: August 2010

Abstract
The present paper describes the method for evaluation of yield strength and fracture toughness of three materials, namely, linepipe steel, aluminum (Al) alloy, and gray cast iron using miniature specimen test. Conventional standard test methods consume more material and money and may damage the integrity of the in-service component. Miniature specimen tests overcome all these demerits. A method is outlined to predict the yield strength and fracture toughness from the miniature test through the empirical relations. The validation of results has been carried out by conducting standard tensile and fracture toughness tests according to ASTM standards. Further, the finite element method has been used to simulate the miniature test. A close agreement is observed between the results of the miniature test and standard tests.
Refinement of Tools for Targeted Gene Expression in Drosophila
Authors: Pfeiffer Barret D , Ngo Teri-T B , Hibbard Karen L , Murphy Christine , Jenett Arnim , Truman James W , Rubin Gerald M 
Published: August 2010

Abstract
A wide variety of biological experiments rely on the ability to express an exogenous gene in a transgenic animal at a defined level and in a spatially and temporally controlled pattern. We describe major improvements of the methods available for achieving this objective in Drosophila melanogaster. We have systematically varied core promoters, UTRs, operator sequences, and transcriptional activating domains used to direct gene expression with the GAL4, LexA, and Split GAL4 transcription factors and the GAL80 transcriptional repressor. The use of site-specific integration allowed us to make quantitative comparisons between different constructs inserted at the same genomic location. We also characterized a set of PhiC31 integration sites for their ability to support transgene expression of both drivers and responders in the nervous system. The increased strength and reliability of these optimized reagents overcome many of the previous limitations of these methods and will facilitate genetic manipulations of greater complexity and sophistication.
c-Type Cytochrome Assembly in Saccharomyces cerevisiae: A Key Residue for Apocytochrome c1/Lyase Interaction
Authors: Corvest Vincent , Murrey Darren A , Bernard Delphine G , Knaff David B , Guiard Bernard , Hamel Patrice P 
Published: August 2010

Abstract
The electron transport chains in the membranes of bacteria and organelles generate proton-motive force essential for ATP production. The c-type cytochromes, defined by the covalent attachment of heme to a CXXCH motif, are key electron carriers in these energy-transducing membranes. In mitochondria, cytochromes c and c1 are assembled by the cytochrome c heme lyases (CCHL and CC1HL) and by Cyc2p, a putative redox protein. A cytochrome c1 mutant with a CAPCH heme-binding site instead of the wild-type CAACH is strictly dependent upon Cyc2p for assembly. In this context, we found that overexpression of CC1HL, as well as mutations of the proline in the CAPCH site to H, L, S, or T residues, can bypass the absence of Cyc2p. The P mutation was postulated to shift the CXXCH motif to an oxidized form, which must be reduced in a Cyc2p-dependent reaction before heme ligation. However, measurement of the redox midpoint potential of apocytochrome c1 indicates that neither the P nor the T residues impact the thermodynamic propensity of the CXXCH motif to occur in a disulfide vs. dithiol form. We show instead that the identity of the second intervening residue in the CXXCH motif is key in determining the CCHL-dependent vs. CC1HL-dependent assembly of holocytochrome c1. We also provide evidence that Cyc2p is dedicated to the CCHL pathway and is not required for the CC1HL-dependent assembly of cytochrome c1.
Role of Prenatal Characteristics and Early Growth on Pubertal Attainment of British Girls
Authors: Maisonet Mildred , Christensen Krista Yorita , Rubin Carol , Holmes Adrianne , Flanders W. Dana , Heron Jon , Ong Ken K. , Golding Jean , McGeehin Michael A. , Marcus Michele 
Published: August 2010

OBJECTIVES:
The objective of this study was to explore the influence of maternal prenatal characteristics and behaviors and of weight and BMI gain during early childhood on the timing of various puberty outcomes in girls who were enrolled in the Avon Longitudinal Study of Parents and Children.

METHODS:
Repeated self-assessments of pubertal development were obtained from ∼4000 girls between the ages of 8 and 14. Data on prenatal characteristics and weight at birth and 2, 9, and 20 months of age were obtained from questionnaires, birth records, and clinic visits. Infants' weights were converted to weight-for-age and BMI SD scores (SDSs; z scores), and change values were obtained for the 0- to 20-month and other intervals within that age range. We used parametric survival models to estimate associations with age of entry into Tanner stages of breast and pubic hair and menarche.

RESULTS:
Maternal initiation of menarche at age &lt;12, smoking during pregnancy, and primiparity were associated with earlier puberty. A 1-unit increase in the weight SDS change values for the 0- to 20-month age interval was associated with earlier ages of entry into pubertal outcomes (0.19–0.31 years). Increases in the BMI SDS change values were also associated with earlier entry into pubertal outcomes (0.07–0.11 years).

CONCLUSIONS:
Many of the maternal prenatal characteristics and weight and BMI gain during infancy seemed to have similar influences across different puberty outcomes. Either such early factors have comparable influences on each of the hormonal processes involved in puberty, or processes are linked and awakening of 1 aspect triggers the others.