bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gprc5a is a novel parathyroid hormone‐inducible gene and negatively regulates osteoblast proliferation and differentiation
Authors: Sampei Chisato , Kato Kosuke , Arasaki Yasuhiro , Kimura Yuta , Konno Takuto , Otsuka Kanon , Kohara Yukihiro , Noda Masaki , Ezura Yoichi , Hayata Tadayoshi 
Published: May 2024

AbstractTeriparatide is a peptide derived from a parathyroid hormone (PTH) and an osteoporosis therapeutic drug with potent bone formation‐promoting activity. To identify novel druggable genes that act downstream of PTH signaling and are potentially involved in bone formation, we screened PTH target genes in mouse osteoblast‐like MC3T3‐E1 cells. Here we show that Gprc5a, encoding an orphan G protein‐coupled receptor, is a novel PTH‐inducible gene and negatively regulates osteoblast proliferation and differentiation. PTH treatment induced Gprc5a expression in MC3T3‐E1 cells, rat osteosarcoma ROS17/2.8 cells, and mouse femurs. Induction of Gprc5a expression by PTH occurred in the absence of protein synthesis and was mediated primarily via the cAMP pathway, suggesting that Gprc5a is a direct target of PTH signaling. Interestingly, Gprc5a expression was induced additively by co‐treatment with PTH and 1α, 25‐dihydroxyvitamin D3 (calcitriol), or retinoic acid in MC3T3‐E1 cells. Reporter analysis of a 1 kb fragment of human GPRC5A promoter revealed that the promoter fragment showed responsiveness to PTH via the cAMP response element, suggesting that GPRC5A is also a PTH‐inducible gene in humans. Gprc5a knockdown promoted cell viability and proliferation, as demonstrated by MTT and BrdU assays. Gprc5a knockdown also promoted osteoblast differentiation, as indicated by gene expression analysis and mineralization assay. Mechanistic studies showed that Gprc5a interacted with BMPR1A and suppressed BMP signaling induced by BMP‐2 and constitutively active BMP receptors, ALK2 (ACVR1) Q207D and ALK3 (BMPR1A) Q233D. Thus, our results suggest that Gprc5a is a novel gene induced by PTH that acts in an inhibitory manner on both cell proliferation and osteoblast differentiation and is a candidate for drug targets for osteoporosis.
Peer evaluation as an alternative assessment method for case presentations: A cross‐sectional study of dental students' perception
Authors: Madi Marwa , Sadaf Shazia , Dawood Zahra Al , Asiri Amal , Farooqi Faraz Ahmed , Abdelsalam Maha , AlHumaid Jehan 
Published: May 2024

AbstractIntroductionPeer assessment in dental and medical education is a key tool for evaluating attitudes and communication skills, serving as an effective formative learning method. It not only boosts academic performance and encourages lifelong learning and reflective practice but also fosters collaboration, effective communication, and constructive feedback among peers.ObjectiveThis study aimed to assess dental students’ satisfaction and perceptions regarding implementing peer assessment in case presentations, including contribution to their learning.MethodsThis cross‐sectional study involved third (n = 26), fourth (n = 54), and sixth (n = 71) dental students divided into five groups. Each student received peer assessments (n = 8) from the students of the other groups regarding their clinical presentation.At the end of the process, students were invited to complete an anonymous 9‐item questionnaire to assess student perceptions of the peer‐assessment process. Data were analyzed to evaluate students’ perception and satisfaction with the peer assessment task. The bivariate analysis explored associations among questionnaire items.ResultsA total of 151 students participated in the current study. Results showed a high level of agreement for most of the students regarding their preparedness for peer and self‐assessment. More than 75% of the students at all academic levels found it challenging to evaluate their peers. Student satisfaction scores increased with the level of education, (4.076, 4.214, and 4.246 out of five, respectively) with moderate correlations between peer evaluation characteristics.ConclusionPeer assessment appears to enhance learning and professional development in dental students. Students’ satisfaction with peer assessment grows with education level, reflecting its role in fostering critical evaluation skills and feedback.
Pulsed lavage is associated with better quality of bone–cement–implant interface in knee arthroplasties (TKA/UKA) compared to syringe lavage in vitro; however, clinical data are missing: A systematic review
Authors: Di Maria Fabrizio , Das Saubhik , Abermann Elisabeth , Hoser Christian , Fink Christian 
Published: May 2024

AbstractPurposeThe purpose of this systematic review is to analyse the available literature to ascertain the optimal method of bone preparation to improve the quality of bone–cement–implant interface with either pulsed lavage or syringe lavage in both total knee arthroplasty (TKA) and unicompartmental knee arthroplasty (UKA).MethodsA comprehensive search was conducted across MEDLINE, Scopus and Embase databases until July 2023. Both inclusion and exclusion criteria were clearly stated and used to identify all the published studies. Subsequent screening throughout the title, abstract and full text was made, followed by complete critical appraisal and data extraction. This sequential process was performed by two reviewers independently and summarised following the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta‐Analyses guidelines). A quality assessment of the systematic review was performed according to the Quality Appraisal for Cadaveric Studies scale (QUACS), reaching a quality level ranging from 69% to 85%.ResultsA total of 10 articles, out of 47, nine biomechanical cadaveric studies and one human clinical study were analysed. A total of 196 UKA tibial components, 74 patellar components, 36 TKA tibial components and 24 UKA femoral components were retrieved, and a high level of heterogeneity resulted overall. The pulsed lavage group showed better cement penetration and higher pull‐out force than the syringe lavage group; a higher interface temperature was also found in the pulsed lavage group. No differences were found regarding tension ligament forces between the groups.ConclusionOur systematic review suggests that pulsed lavage is superior to syringe lavage in terms of the quality of bone–cement–implant interface in knee arthroplasties (TKA/UKA). However, translation of these results from cadaveric studies to individual clinical settings may be hazardous; therefore, clinical in vivo prospective studies are highly needed.PROSPERO CRDPROSPERO CRD number CRD42023432399Level of EvidenceLevel III.
Promising results following derotational femoral osteotomy in patellofemoral instability with increased femoral anteversion: A systematic review on current indications, outcomes and complication rate
Authors: Klasan Antonio , Compagnoni Riccardo , Grassi Alberto , Menetrey Jacques 
Published: May 2024

AbstractPurposePatellofemoral joint instability (PFJI) can surgically be treated with a multitude of approaches, depending on the underlying pathology. In the presence of increased femoral anteversion, some authors have reported good results with a derotational distal femoral osteotomy (DeDFO). The purpose of the study was to investigate the indications, outcomes and complication rate of DeDFO for PFJI.MethodsA systematic review was performed according to the PRISMA guidelines (Preferred Reporting Items for Systematic Reviews and Meta‐analyses) by searching Medline, Embase, Web of Science and Cochrane Library databases through 1 December 2023. Included were levels 1–4 clinical studies of skeletally mature patients undergoing a DeDFO for PFJI irrespective of concomitant procedures. Study characteristics, indications, radiological and clinical outcomes, surgical technique and concomitant procedures, re‐dislocation and complication rate were all analysed, as was methodological quality.ResultsA total of 12 studies including 310 patients (325 knees) were included. Three studies were cohort studies, all others were case series. The mean patient age across the studies was 22 years, and the mean follow‐up was 29.4 months. Femoral anteversion cut‐off was between 20° and 30°. Every study included at least one concurrent soft tissue, bony or combined procedure. Across all studies, one case of re‐dislocation was reported (0.3%) and four implant or osteotomy‐related complications (1.2%) were reported. All studies reported a statistically significant increase in clinical scores.ConclusionThis systematic review of DeDFO for patellofemoral instability in the presence of increased femoral anteversion demonstrates promising clinical results and an extremely low dislocation and complication rate. The heterogeneity of the cut‐off in anteversion and concomitant procedures, especially tibial tubercle osteotomy with seemingly identical results, indicates the need for high‐quality evidence for treating patellofemoral instability. Based upon this systematic review, we strongly recommend that DeDFO be added to the ‘menu à la carte’ of PFJI.Level of EvidenceLevel III Systematic Review.
A review on pyrimidine‐based derivatives: Synthesis and their biological application
Authors: Islam Md. Wahidul , Islam Md. Monarul , Akter Rabeya , Limon Tayebur Rahman , Vasquez Erick S. , Shaikh Md. Aftab Ali , Habib Ahsan 
Published: May 2024

AbstractPyrimidine‐based derivatives have attracted a lot of interest in heterocyclic chemistry due to its versatile structure and diverse biological activities. This study provides a comprehensive overview of synthetic strategy of different pyrimidine ring and highlights their biological importance. In this article, we start off by going through the fundamental procedures for the synthesis of pyrimidine scaffolds, including both conventional and contemporary synthetic techniques. These works cover a range of therapeutic domains, such as effects that are anticancer, antibacterial, antiviral, anti‐inflammatory, antioxidant, antimalarial, and so on. Moreover, we have summarized with a discussion of future prospects and challenges in the field of pyrimidine heterocyclic chemistry. This thorough review is a significant resource for researchers in medicinal chemistry and related fields since it offers insightful information about the synthetic methods and biological applications of pyrimidine‐based derivatives.
Phytochemical inhibitors from Leucas aspera against the target proteins induced by Trichophyton mentagrophytes using computational techniques
Authors: Giri Monalisha , Parida Sagarika 
Published: May 2024

AbstractDermatophytosis is a cutaneous infection that is able to degrade the keratinized tissues of the animal/human body, like skin, nails, and hair, causing chronic or subacute infection with the contact of some specific fungal strains. Trichophyton mentagrophytes are the most potential fungal pathogen causing dermatophytoses. The present study focuses on computationally based in silico antifungal activity of selected phytocompounds of Leucas aspera (Willd.) Link. against dermatophytic fungus, T. mentagrophytes. Validation and screening of derived phytocompounds is performed using Lipinski rule of five and toxicity test through Protox‐II. Five target genes involved in dermatophytosis, induced by T. mentagrophytes are retrieved from the UniProt Database, and the corresponding proteins such as glucan 1,3‐beta‐glucosidase ARB_02797, Probable class II chitinase ARB_00204, squalene monooxygenase, actin, and ubiquitin are selected for in silico study. Three‐dimensional structures of the target protein were computationally determined and validated through modeling tools and techniques due to the lack of validated protein structures in the database. Then, these proteins are used for in silico molecular docking through the AutoDock Vina tool to find out the promising phytocompounds. This study could be utilized in designing more effective drugs against T. mentagrophytes. Based on this work, a plant‐based natural alternative can be added to the treatment of dermatophytosis rather than synthetic supplements.
The impact of project‐based interorganizational networks: Evidence from the Chinese movie industry
Authors: Li Lingjia , Shou Yongyi 
Published: May 2024

AbstractProject‐based interorganizational networks are temporary and dynamic, and significantly different from stable networks for mass production. However, empirical studies on project‐based interorganizational networks are scant. Drawing upon the network literature and distinguishing between production and distribution networks, we investigate how the centrality of a project in industry networks and tie strength of the project's member firms affect project performance in terms of product quality and sales. Using data of 821 Chinese movie projects during 2015–2018, we find empirical evidence that a project's centrality in the production network enhances product quality and that in the distribution network elevates product sales. Interestingly, weak ties in the production team contribute to product quality while strong ties in the distribution team improve product sales. This study advances the literature on project‐based industries and interorganizational networks. It highlights the distinction between production and distribution networks in project‐based industries and sheds light on the differential roles of a project's centrality and tie strength in both networks. These findings also offer novel insights to managers in project‐based industries.
Effects of bamboo leaf flavonoids on growth performance, antioxidants, immune function, intestinal morphology, and cecal microbiota in broilers
Authors: Wu Chao , Ma Hui , Lu Shuwan , Shi Xueyan , Liu Jinsong , Yang Caimei , Zhang Ruiqiang 
Published: May 2024

AbstractBACKGROUNDBamboo leaf flavonoids (BLF) are the main bioactive ingredients in bamboo leaves. They have antioxidant, anti‐inflammatory, antibacterial, and other effects. In this study, the effects of dietary BLF on growth performance, immune response, antioxidant capacity, and intestinal microbiota of broilers were investigated. A total of 288 broilers were divided into three groups with eight replicates and 12 birds in each replicate. Broilers were fed a basic diet or the basic diet supplemented with 1000 or 2000 mg kg−1 BLF for 56 days.RESULTSThe results showed that supplementation of BLF increased body weight (BW) and average daily weight gain (ADG), and reduced average daily feed intake (ADFI) (P < 0.05). The serum immunoglobulin A (IgA), immunoglobulin M (IgM), and interleukin 10 (IL‐10) content of broilers in the BLF1000 group was increased and the interleukin 1β (IL‐1β) and tumor necrosis factor‐α (TNF‐α) content was decreased (P < 0.05). The levels of IgM and IL‐10 in jejunum mucosa were found to be enhanced by BLF (P < 0.05). The BLF1000 group exhibited a significant reduction in the concentration of TNF‐α (P < 0.05). Serum and jejunum mucosa total antioxidant capacity (T‐AOC) levels in the BLF1000 group were increased (P < 0.05). The serum catalase (CAT) and glutathione peroxidase (GSH‐Px) effects of the BLF1000 group and serum CAT effects of BLF2000 group were increased (P < 0.05). The CON group demonstrated a lower relative abundance of Christensenellaceae_R‐7_group and Oscillibacter than the BLF group (P < 0.05).CONCLUSIONDietary BLF inclusion enhanced the growth performance, immune, and antioxidant functions, improved the intestinal morphology, and ameliorated the intestinal microflora structure in broiler. Adding 1000 mg kg−1 BLF to the broiler diet can be considered as an effective growth promoter. © 2024 Society of Chemical Industry.
On non‐hydrogen‐atom products of thermal decomposition of benzyl radical: A theoretical investigation by the transition state theory/multi‐well master equation approach
Authors: Meng Qinghui , Chi Yicheng , Zhang Lidong , Zhang Peng 
Published: May 2024

AbstractBenzyl radical (C7H7), one of the resonantly stabilized hydrocarbon radicals, is one of the significant precursors of polycyclic aromatic hydrocarbons in interstellar media and combustion engines. The unimolecular decomposition of benzyl radical is still incompletely understood despite of its importance and relatively small molecular size. The decomposition reactions of benzyl radical were investigated in the present study by using the ab initio transition state theory (TST) and the multi‐well master equation theory. Specifically, all reaction pathways on the potential energy surface of C7H7 was calculated at the level of QCISD(T)/CBS. For the reactions with multireference characters, the CASPT2(9e,7o)/aug‐cc‐pVTZ method was used to calculate the vibrational frequencies and energies of structures along the one‐dimensional reaction coordinate of the breaking bond. The high‐pressure limits of rate constants for all the reactions were obtained by using the TST except those for C7H6 + H and C6H4 + CH3 by the variational TST. The pressure‐dependent rate constants were obtained by using the multi‐well master equation simulations. The calculated rate constants agree well with available experimental and theoretical data in the literature. Moreover, the present results identify the composition of the non‐hydrogen‐atom production observed in previous experiments, which provide new insights into the reactions of aromatic compounds.
Assessment of eco‐environmental changes along roads in the Qinghai‐Tibetan Plateau based on remote sensing data: A case study of the Sino‐Nepalese Transport Corridor
Authors: Sun Yaohang , Jin Gang , Zhao Liangsheng , Guo Jianjun , Yue Dongxia 
Published: May 2024

AbstractUnderstanding the evolutionary patterns of ecological quality in transport corridor areas and their driving mechanisms is essential for maintaining regional sustainable development. However, an integrated assessment of the ecology of plateau transport corridors is still lacking. This study focuses on the Sino‐Nepalese Transport Corridor (SNTC) and comprehensively evaluates the spatio‐temporal patterns and evolution of eco‐environmental quality from 2000 to 2021 based on remote sensing data and quantifies and explores the potential driving factors. The results indicate significant spatial heterogeneity in the eco‐environmental quality of the SNTC, with an overall bipolar “northeast‐southwest” distribution pattern. The area of poor and worse quality is the largest (32,471.75 km2), accounting for about 47.20% of the total area of the SNTC, distributed in the plateau bare land and the Yarlung Tsangpo River valley. From 2000 to 2021, the eco‐environmental quality showed an initial decline and then an increase, with a relatively gentle overall trend. The increase in the remote sensing ecological index in Region (I) from 0.314 in the first 10 years to 0.334 in the second 10 years, an increase of 6.37%, was mainly attributed to afforestation projects in the “One River and two tributaries Nature Reserve” region. The eco‐environmental quality of the SNTC is influenced by multiple factors including human activities, climate, and topography. Grazing intensity, population density, and digital elevation model are the main negative disturbance factors, while temperature, precipitation, gross domestic product, and afforestation areas promote eco‐environmental quality. In particular, afforestation projects partially offset the negative impact of human activities. These results will provide useful information for understanding the complex effects of human activities and natural factors on the eco‐environmental quality in the plateau transport corridor region and for formulating ecological conservation policies.
Effects of different lipid sources with or without a probiotic on gastrointestinal tract, immune system and blood parameters of chickens: An animal model
Authors: Poorghasemi Mohammadreza , Chamani Mohammad , Mirhosseini Seyed Ziaeddin , Saravy Ali , Sadeghi Ali‐Asghar , Seidavi Alireza , D'Avila Lima Heder José 
Published: May 2024

AbstractThe objective of this study was to determine the effects of different lipid sources, with or without a probiotic, on the gastrointestinal tract, immune system and blood parameters of Ross 308 male chickens. In this study, 360 one‐day‐old chickens were randomly allotted to six treatments with six replicates. Experimental diets were: (1) control (CTL); (2) a diet containing 30 g/kg lipid from tallow (CTL+TLW); (3) a diet containing 30 g/kg lipid from soybean oil (CTL+SO); (4) the basal diet plus a probiotic (CTL+PRO), (5) a diet containing 30 g/kg tallow plus probiotic (TLW+PRO); and (6) a diet containing 30 g/kg soybean oil plus probiotic (SO+PRO). The percentage of liver and jejunum in the treatments that used tallow alone or tallow with probiotics had a significant increase as compared to the control. The villus height and crypt depth of the ileum and villus height/crypt depth in the treatments that used soybean oil and probiotic alone had a significant increase compared to the control. The weight of the spleen, bursa of Fabricius, and thymus in the treatments that used probiotics had a significant increase compared to the control. The amount of alkaline phosphatase and alanine aminotransferase as well as triacylglycerol in the treatment containing probiotic and its mixture with soybean oil had the least significant difference with the control. The results showed that the use of soybean oil, probiotics, and their mixture can improve intestinal morphology, strengthen the immune system, and reduce liver enzymes in chickens.
A platinum(IV)–artesunate complex triggers ferroptosis by boosting cytoplasmic and mitochondrial lipid peroxidation to enhance tumor immunotherapy
Authors: Fan Renming , Deng Aohua , Lin Ruizhuo , Zhang Shuo , Cheng Caiyan , Zhuang Junyan , Hai Yongrui , Zhao Minggao , Yang Le , Wei Gaofei 
Published: May 2024

AbstractFerroptosis is an iron‐dependent cell death form that initiates lipid peroxidation (LPO) in tumors. In recent years, there has been growing interest on ferroptosis, but how to propel it forward translational medicine remains in mist. Although experimental ferroptosis inducers such as RSL3 and erastin have demonstrated bioactivity in vitro, the poor antitumor outcome in animal model limits their development. In this study, we reveal a novel ferroptosis inducer, oxaliplatin–artesunate (OART), which exhibits substantial bioactivity in vitro and vivo, and we verify its feasibility in cancer immunotherapy. For mechanism, OART induces cytoplasmic and mitochondrial LPO to promote tumor ferroptosis, via inhibiting glutathione‐mediated ferroptosis defense system, enhancing iron‐dependent Fenton reaction, and initiating mitochondrial LPO. The destroyed mitochondrial membrane potential, disturbed mitochondrial fusion and fission, as well as downregulation of dihydroorotate dehydrogenase mutually contribute to mitochondrial LPO. Consequently, OART enhances tumor immunogenicity by releasing damage associated molecular patterns and promoting antigen presenting cells maturation, thereby transforming tumor environment from immunosuppressive to immunosensitive. By establishing in vivo model of tumorigenesis and lung metastasis, we verified that OART improves the systematic immune response. In summary, OART has enormous clinical potential for ferroptosis‐based cancer therapy in translational medicine.