bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulation of innate immunity by the nucleotide pathway in children with idiopathic nephrotic syndrome
Authors: Bertelli R , Bodria M , Nobile M , Alloisio S , Barbieri R , Montobbio G , Patrone P , Ghiggeri G M 
Published: July 2011

SummaryActivation of the oxidative burst and failure of CD4+CD25+ cell regulation have been implicated in idiopathic nephrotic syndrome (iNS). The intimate mechanism is, however, unknown and requires specifically focused studies. We investigated reactive oxygen species (ROS) generation [di-chlorofluorescein-diacetate (DCFDA)] fluorescence assay and the regulatory adenosine 5′-triphosphate (ATP) pathways in the blood of 41 children with iNS, utilizing several agonists and antagonists of nucleotide/nucleoside receptors, including the addition of soluble apyrase. The CD4+CD25+CD39+/CD73+ expression was determined in vivo in parallel during disease activity. Overall, we found that the percentage of CD39+CD4+CD25+ was reduced markedly in iNS by 80% (3·43 ± 0·04% versus 13·14 ± 0·07% of total lymphocytes, P &lt; 0·001). In these patients, reactive oxygen species (ROS) generation by polymorphonuclear neutrophils (PMN) at rest was a function of apyrase (CD39) expressed by CD4+CD25+, with higher rates in patients with very low CD39+CD4+CD25+ levels (&lt; 7·5%). Addition of apyrase reduced ROS generation by 40% in both iNS and controls and was mainly effective in patients. The quota of ROS surviving ATP elimination was higher still in iNS. In vitro studies to limit ROS generation with adenosine analogues (2′-chloroadenosine and 5′-N-ethylcarboxamidoadenosine) produced minor effects. At variance, antagonizing ATP efflux with carbenoxolone or by antagonizing ATP effects (Brilliant Blue G, KN62 and A437089) reduced ROS generation comparable to apyrase. These results confirm a key role of ATP in the regulation of innate immunity and minimize the effect of adenosine. Decreased CD39+CD4+CD25+ expression in iNS highlights an impairment of ATP degradation in this pathology. However, high ROS surviving ATP consumption implies a major role of other regulatory pathways.
Tumour necrosis factor-like weak inducer of apoptosis (TWEAK), an important mediator of endothelial inflammation, is associated with the pathogenesis of Henoch–Schonlein purpura
Authors: Chen T , Guo Z-P , Li M-M , Li J-Y , Jiao X-Y , Zhang Y-H , Liu H-J 
Published: July 2011

Summary
Tumour necrosis factor-like weak inducer of apoptosis (TWEAK), a member of the tumour necrosis factor (TNF) family, has been implicated as a proinflammatory cytokine in many types of autoimmune and infectious diseases. However, information about TWEAK in dermatological diseases is limited. Herein, we investigated the role of TWEAK in patients with Henoch–Schonlein purpura (HSP) and the ability of TWEAK on chemokine production in the human dermal microvascular endothelial cell line (HMEC-1). Serum TWEAK levels in patients with HSP, together with patients with psoriasis vulgaris (PV) and atopic dermatitis (AD), were detected by enzyme-linked immunosorbent assay (ELISA). HMEC-1 cells were treated with TWEAK at concentrations ranging from 1 ng/ml to 100 ng/ml. Serum levels of TWEAK were elevated in patients with HSP in the acute stage but not in patients with PV or AD. Moreover, TWEAK levels were correlated with the severity of HSP. TWEAK markedly induced CCL5 and CXCL8 production at both mRNA and protein levels in HMEC-1 cells. In addition, TWEAK-stimulated HMEC-1 supernatant enhanced HL-60 or human acute monocytic leukaemia cell line (THP-1) cell migration. Finally, Western blot data revealed that TWEAK can induce rapid phosphorylation of inhibitor of κB-α (IκBα) in HMEC-1 cells. In conclusion, we show that serum levels of TWEAK were elevated in patients with acute stage HSP. TWEAK may act as a regulator of nuclear factor-κB (NF-κB) activation and chemokine production in human dermal microvascular endothelial cells, thus promoting leucocyte migration in cutaneous vasculitis.
Bringing immunoglobulin knowledge up to date: how should we treat today?
Authors: Misbah S , Kuijpers T , van der Heijden J , Grimbacher B , Guzman D , Orange J 
Published: July 2011

Summary
Immunoglobulin (Ig) therapy is constantly evolving. Advances in the basic and clinical science of immunoglobulins have provided new perspectives in using polyclonal IgG to treat patients with primary immunodeficiencies. Recent meta-analyses of patient data and outcomes, optimization of IgG administration and better understanding of the IgG receptor variability and clinical effect are new concepts which practising immunologists can use in tailoring their approach to treating patients with primary immunodeficiencies. This manuscript presents the proceedings of a satellite symposium, held in conjunction with the European Society for Immunodeficiencies (ESID) 2010 meeting, to inform attendees about new scientific concepts in IgG therapy, with the goal of empowering expert level evaluation of what optimal IgG therapy is today.
Tulathromycin assay validation and tissue residues after single and multiple subcutaneous injections in domestic goats (Capra aegagrus hircus)
Authors: CLOTHIER K. A. , LEAVENS T. , GRIFFITH R. W. , WETZLICH S. E. , BAYNES R. E. , RIVIERE J. E. , TELL L. A. 
Published: July 2011

Clothier, K. A., Leavens, T., Griffith, R. W., Wetzlich, S. E., Baynes, R. E., Riviere, J. E., Tell, L. A. Tulathromycin assay validation and tissue residues after single and multiple subcutaneous injections in domestic goats (Capra aegagrus hircus). J. vet. Pharmacol. Therap. 35, 113–120.Tulathromycin is a macrolide antimicrobial labeled for treatment of bacterial pneumonia in cattle and swine. The purpose of the present research was to evaluate tissue concentrations of tulathromycin in the caprine species. A tandem mass spectrometry regulatory analytical method that detects the common fragment of tulathromycin in cattle and swine was validated with goat tissues. The method was used to study tulathromycin depletion in goat tissues (liver, kidney, muscle, fat, injection site, and lung) over time. In two different studies, six juvenile and 25 market‐age goats received a single injection of 2.5 mg/kg of tulathromycin subcutaneously; in a third study, 18 juvenile goats were treated with 2.5, 7.5, or 12.5 mg/kg tulathromycin weekly with three subcutaneous injections. Mean tulathromycin tissue concentrations were highest at injection site samples in all studies and all doses. Lung tissue concentrations were greatest at day 5 in market‐age goats while in the multi‐dose animals concentrations demonstrated dose‐dependent increases. Concentrations were below limit of quantification in injection site and lung by day 18 and in liver, kidney, muscle, and fat at all time points. This study demonstrated that tissue levels in goats are very similar to those seen in swine and cattle.
Extended access cocaine self‐administration differentially activates dorsal raphe and amygdala corticotropin‐releasing factor systems in rats
Authors: Zorrilla Eric P. , Wee Sunmee , Zhao Yu , Specio Sheila , Boutrel Benjamin , Koob George F. , Weiss Friedbert 
Published: July 2011

ABSTRACTCocaine‐induced neuroadaptation of stress‐related circuitry and increased access to cocaine each putatively contribute to the transition from cocaine use to cocaine dependence. The present study tested the hypothesis that rats receiving extended versus brief daily access to cocaine would exhibit regional differences in levels of the stress‐regulatory neuropeptide corticotropin‐releasing factor (CRF). A secondary goal was to explore how CRF levels change in relation to the time since cocaine self‐administration. Male Wistar rats acquired operant self‐administration of cocaine and were assigned to receive daily long access (6 hours/day, LgA, n = 20) or short access (1 hour/day, ShA, n = 18) to intravenous cocaine self‐administration (fixed ratio 1, ∼0.50 mg/kg/infusion). After at least 3 weeks, tissue CRF immunoreactivity was measured at one of three timepoints: pre‐session, post‐session or 3 hours post‐session. LgA, but not ShA, rats showed increased total session and first‐hour cocaine intake. CRF immunoreactivity increased within the dorsal raphe (DR) and basolateral, but not central, nucleus of the amygdala (BLA, CeA) of ShA rats from pre‐session to 3 hours post‐session. In LgA rats, CRF immunoreactivity increased from pre‐session to 3 hours post‐session within the CeA and DR but tended to decrease in the BLA. LgA rats showed higher CRF levels than ShA rats in the DR and, pre‐session, in the BLA. Thus, voluntary cocaine intake engages stress‐regulatory CRF systems of the DR and amygdala. Increased availability of cocaine promotes greater tissue CRF levels in these extrahypothalamic brain regions, changes associated here with a model of cocaine dependence.
Positioning positivism, critical realism and social constructionism in the health sciences: a philosophical orientation
Authors: Cruickshank Justin 
Published: July 2011

CRUICKSHANK J. Nursing Inquiry 2012; 19: 71–82 Positioning positivism, critical realism and social constructionism in the health sciences: a philosophical orientationThis article starts by considering the differences within the positivist tradition and then it moves on to compare two of the most prominent schools of postpositivism, namely critical realism and social constructionism. Critical realists hold, with positivism, that knowledge should be positively applied, but reject the positivist method for doing this, arguing that causal explanations have to be based not on empirical regularities but on references to unobservable structures. Social constructionists take a different approach to postpositivism and endorse a relativist rejection of truth and hold that the task of research is to foster a scepticism that undermines any positive truth claim made. It is argued that social constructionism is a contradictory position.
Ecological selection and sexual dimorphism in the sooty oystercatcher, Haematopus fuliginosus
Authors: APLIN LUCY MARGARET , COCKBURN ANDREW 
Published: July 2011

AbstractMale and female sooty oystercatchers (subspecies Haematopus fuliginosus fuliginosus; Haematopodidae) have an average difference in bill length of 19%. We studied the relationship between this sexual dimorphism and foraging ecology at coastal sites in southern New South Wales, Australia. Intersexual foraging divergence was most striking in diet, with seven prey classes eaten exclusively by one sex (male: 4, female: 3), and all shared prey classes eaten in different proportions. Intersexual diet partitioning was also observed in energetic rewards gained from foraging, with females gaining highest energetic benefits from eating ascidians and males from eating limpets. Furthermore, within the most commonly consumed prey item, limpets, females gained higher energetic benefit from eating smaller sizes while males gained greater rewards from the largest limpet sizes. Intersexual divergence was also observed in several aspects of foraging behaviour. Finally, there was a significant effect of tidal cycles upon intersexual niche partitioning in this species; the degree of diet divergence varied between tide conditions and females had a consistently more efficient dietary intake on neap tides than males. Diet divergence in the sooty oystercatcher is greater than previously observed in any oystercatcher, and is correlated with the largest sexual bill dimorphism recorded in this family. It is argued that intersexual competition between territorial pairs is operating to diverge male and female bill morphology.
Mechanisms of rainforest persistence and recruitment in frequently burnt wet tropical eucalypt forests
Authors: WILLIAMS PAUL R. , PARSONS MARK , JENSEN RIGEL , TRAN CUONG 
Published: July 2011

AbstractThe role of fire in governing rainforest–eucalypt forest ecotone dynamics is of theoretical interest and has conservation management implications. Several eucalypt forests in the Wet Tropics of Australia have an endangered status due to extensive conversion to rainforest. Rainforest plants are known to survive occasional low intensity fires in the eucalypt forest ecotone. However, the ability of rainforest plants to survive frequent fires remains untested. The timing of rainforest expansion is also a subject of interest, and is generally considered to be delayed until fire has been absent for several years. We used 14 years of data collected across 13 plots in the Wet Tropics of north‐eastern Australia to test predictions regarding rainforest seedling recruitment and post‐fire regenerative capacity. The 13 plots received different numbers of fires, between zero and five, over the 14‐year study. The recruitment of new rainforest plants in the ecotone was most abundant in the initial year after fire. If this post‐fire pulse of recruitment is left undisturbed, it can facilitate the subsequent germination of additional rainforest species. The removal of grass cover, whether temporarily in the immediate post‐fire environment or once a developing rainforest mid strata shades out grasses, appears crucial to abundant rainforest recruitment. A variety of tropical rainforest species can persist under a frequent fire regime through resprouting. The difference in the mode of resprouting, between ground‐level coppicing rainforest plants and canopy resprouting eucalypt forest trees, is the critical mechanism that causes regular fire to maintain an open structure in eucalypt forests. The inability of rainforest species to maintain their height when fires fully scorch their crowns, temporarily resets the forest's open structure and delays the rainforest's ability to dominate through shading out grasses to transform the ecosystem into a closed forest.
Symptomatic 5‐fluorouracil‐induced sinus bradycardia
Authors: Lee A. D. , McKay M. J. 
Published: July 2011

Abstract5‐Fluorouracil (5‐FU) is a commonly used anti‐neoplastic agent. 5‐FU has been not uncommonly associated with cardiotoxicity, although the many potentially causative mechanisms are yet to be established. Here, we present the case of a 61‐year‐old gemstone miner who developed symptomatic sinus bradycardia while receiving a continuous 5‐FU infusion combined with radiotherapy for locally advanced rectal cancer. This dysrhythmia is an unusual type of 5‐FU toxicity, our case being the second described. We review the actions of 5‐FU and the various proposed mechanisms of its cardiotoxic effects.
Random movement pattern of fed and unfed adult Colorado potato beetles in bare‐ground habitat
Authors: Gui Lian‐You , Boiteau Gilles , Colpitts Bruce G. , MacKinley Pamela , McCarthy Peter C. 
Published: July 2011

Although the successful management of the Colorado potato beetle Leptinotarsa decemlineata (Say) depends on the prevention of its dispersal, its walking pattern in the landscape remains poorly understood. In the present study, post‐diapause, early summer, late summer and colony adult beetles, both fed and unfed before release, were tracked with a harmonic radar to establish their walking movement pattern in a bare‐ground field.
The random walk model successfully described the dispersal of all beetle types, whether fed or unfed.
The diverse life history of this species was manifested by an increased distance travelled and deviations of individual paths from the random model. Starved post‐diapause beetles travelled furthest and individual paths deviating from random were both local and directed, probably aiming to maximize opportunities for host colonization. Starved early summer beetles also travelled further than fed beetles but relied more on random movement to disperse in the habitat. Starving had little impact on the distance travelled or the path deviations of late summer beetles that are searching for overwintering site rather than hosts.
The increased displacement of starving beetles over fed beetles corresponded with an increased walking step and index of straightness.
The impact of starvation on travel distance was greater than expected from laboratory tests.
In conclusion, the results obtained in the present study suggest a random walking pattern to search arable land until host volatile or visual impulses trigger a more directed walk or flight.
Pollination efficiency of wild bees and hoverflies provided to oilseed rape
Authors: Jauker Frank , Bondarenko Birgit , Becker Heiko C. , Steffan‐Dewenter Ingolf 
Published: July 2011

Declining numbers in honeybees and various wild bee species pose a threat to global pollination services. The identification and quantification of the pollination service provided by different taxa within the pollinator guild is a prerequisite for the successful establishment of nature conservation and crop management regimes.
Wild bees and hoverflies are considered to be valuable pollinators in agricultural and natural systems. Although some information on pollination efficiency of individual pollinator species is available, comparative studies of both taxa at different densities are rare. In the present study, the efficiency of the solitary mason bee Osmia rufa and two hoverfly species (Eristalis tenax and Episyrphus balteatus) as pollinators of oilseed rape Brassica napus was examined in a standardized caged plant breeding regime. Honeybee Apis mellifera colonies were used as a reference pollinator taxon.
Yield parameters responded differently to pollinator density and identity. Fruit set and number of seeds per pod increased with increasing pollinator density, although these were stronger in the mason bee than the hoverfly treatment. Weight per 1000 seeds did not respond to any pollinator treatment, indicating that seed quality was not affected. Oilseed rape yield in the highest tested densities of both pollinator taxa resulted in yield values close to the efficiency of small honeybee colonies.
Hoverflies required approximately five‐fold densities of the red mason bees to reach a similar fruit set and yield. Thus, mason bees are more efficient in plant breeding and managed pollination systems. Both natural pollinator taxa, however, are of potential value in open and closed crop production systems.
Seafood mislabelling: comparisons of two western European case studies assist in defining influencing factors, mechanisms and motives
Authors: Miller Dana , Jessel Adam , Mariani Stefano 
Published: July 2011

AbstractThe global seafood industry, influenced by consumer demand, is closely linked to the global fishing industry, which determines the variety of fish available for consumption. The recently revealed issue of seafood mislabelling threatens to weaken this link by removing consumer power to influence patterns of fisheries exploitation through informed choice. Recognizing this, there is an urgent need to go beyond the mere documentation of the phenomenon and learn more about the origins of this problem and the nature of factors influencing its occurrence to develop solutions. In an attempt to understand seafood mislabelling more thoroughly in Europe, 226 cod products were purchased from Ireland and the UK, genetically identified using a DNA barcoding technique (COI barcoding gene), and species identification results were compared against product labels. Cod mislabelling proved more severe in Ireland than in the UK (28.4% vs. 7.4%). Moreover, whereas data show that in Ireland, cheaper species are sold as cod, in the UK, threatened Atlantic cod (Gadus morhua) may be sold as ‘sustainably sourced’ Pacific cod. Considering these countries operate under the same EU policies for seafood traceability and labelling, it is likely that this situation has been influenced by heightened consumer awareness in the UK, which has created an environment where mislabelling is discouraged. In addition to identifying samples, traceability information from packaged cod was used to trace products back to supplying companies. Although inconclusive in determining blame, this exercise has demonstrated that using traceability information can add explanatory power when attempting to determine responsibility for the occurrence of mislabelling.
The Sensitivity of Respondent-Driven Sampling
Authors: Lu Xin , Bengtsson Linus , Britton Tom , Camitz Martin , Kim Beom Jun , Thorson Anna , Liljeros Fredrik 
Published: July 2011

SummaryResearchers in many scientific fields make inferences from individuals to larger groups. For many groups, however, there is no list of members from which to draw a random sample. Respondent-driven sampling (RDS) is a relatively new sampling methodology that circumvents this difficulty by using the social networks of the groups under study. The RDS method has been shown to provide unbiased estimates of population proportions given certain conditions. The method is now widely used in human immunodeficiency virus related studies among high risk populations globally. We test the RDS methodology by simulating RDS studies on the social networks of a large Lesbian, gay, bisexual and transgender Web community. The robustness of the RDS method is tested by violating, one by one, the conditions under which the method provides unbiased estimates. Simulations indicate that the bias is large if networks are directed or respondents choose to invite people on the basis of characteristics that are correlated with the study outcomes. The bias and variance increase if participants invite close as opposed to more distant friends whereas sampling in denser networks sharply reduces variance. However, the RDS method shows strong resistance to sampling without replacement, low response rates and certain errors in the participants’ reporting of their network sizes, as well as the selection criteria of seeds. The effects of network structure and the number of seeds and coupons are also discussed.
THE COMMUNICATIVE POTENTIAL OF THERAN MURALS IN LATE BRONZE AGE AKROTIRI: APPLYING VIEWSHED ANALYSIS IN 3D TOWNSCAPES
Authors: PALIOU ELEFTHERIA 
Published: July 2011

SummaryThis paper aims to contribute to the discussion of the social functions of Aegean wall painting, by examining the communicative impact and possible socio‐symbolic significance of Theran murals in the urban landscape of Late Bronze Age Akrotiri (Thera, Greece). It uses a novel method of computational analysis to investigate the visibility of mural painting in the prehistoric townscape which combines the functionalities of 3D modelling and Geographic Information Systems (GIS). The benefits of this approach lie in that it considers virtually all observer locations in the study area, while taking more fully into account the precise shape of built forms and the physiological structure of the human visual system than other established methods of visibility analysis used in landscape and urban studies. The application of the methodology in the townscape of Akrotiri highlights some previously unobserved spatial relationships that could have played a role in enhancing the communicative impact of Theran murals in the LBA built environment, consequently encouraging the wide production of mural decoration in the settlement.
PAST ‘DISTURBANCES’ OF GRAVES AS A SOURCE: TAPHONOMY AND INTERPRETATION OF REOPENED EARLY MEDIEVAL INHUMATION GRAVES AT BRUNN AM GEBIRGE (AUSTRIA) AND WINNALL II (ENGLAND)
Authors: ASPÖCK EDELTRAUD 
Published: July 2011

SummaryEarly medieval graves that were reopened in the past are usually considered ‘disturbed’ and hence an unreliable source for traditional cemetery analysis. This paper aims to highlight how the analysis of these ‘disturbances’ can contribute to our understanding of early medieval mortuary rites and attitudes towards the buried human body. Two case studies of cemeteries with high proportions of reopened graves are presented. Thorough archaeological analysis, with careful consideration of the taphonomy of reopened graves, is the key to an understanding of the reopening practices. At Brunn am Gebirge (Austria) most graves were reopened for ‘grave‐robbery’– to remove grave goods – at a time when the bodies were already fully disarticulated. The graves at Winnall II (England) were reopened very soon after burial to manipulate the still largely intact corpses.