bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Forkhead factor FoxQ1 influences epithelial differentiation
Authors: Feuerborn A. , Srivastava P.K. , Küffer S. , Grandy W.A. , Sijmonsma T.P. , Gretz N. , Brors B. , Gröne H.‐J. 
Published: August 2010

AbstractThe Forkhead family of transcription factors comprises numerous members and is implicated in various cellular functions, including cell growth, apoptosis, migration, and differentiation. In this study, we identified the Forkhead factor FoxQ1 as increased in expression during TGF‐β1 induced changes in epithelial differentiation, suggesting functional roles of FoxQ1 for epithelial plasticity. The repression of FoxQ1 in mammary epithelial cells led to a change in cell morphology characterized by an increase in cell size, pronounced cell–cell contacts, and an increased expression of several junction proteins (e.g., E‐cadherin). In addition, FoxQ1 knock‐down cells revealed rearrangements in the actin‐cytoskeleton and slowed down cell cycle G1‐phase progression. Furthermore, repression of FoxQ1 enhanced the migratory capacity of coherent mammary epithelial cells. Gene expression profiling of NM18 cells indicated that FoxQ1 is a relevant downstream mediator of TGF‐β1‐induced gene expression changes. This included the differential expression of transcription factors involved in epithelial plasticity, for example, Ets‐1, Zeb1, and Zeb2. In summary, this study has elucidated the functional impact of FoxQ1 on epithelial differentiation. J. Cell. Physiol. 226: 710–719, 2011. © 2010 Wiley‐Liss, Inc.
Hyperosmolarity‐mediated mitochondrial dysfunction requires Transglutaminase‐2 in human Corneal epithelial cells
Authors: Png Evelyn , Samivelu G.K. , Yeo S.H. , Chew J. , Chaurasia Shyam S. , Tong Louis 
Published: August 2010

AbstractHyperosmolar‐induced ocular surface cell death is a key mitochondria‐mediated event in inflammatory eye diseases. Transglutaminase (TGM)‐2, a cross‐linking enzyme, is purported to mediate cell death, but its link to mitochondria is unclear. In the cornea, the integrity of the epithelial cells is important for maintaining transparency of the cornea and therefore functional vision. We evaluated the role of TGM‐2 and its involvement in hyperosmolarity‐stimulated mitochondrial cell death in human corneal epithelial (HCE‐T) cells. HCE‐T cell lines stably expressing either shRNA targeting TGM‐2 (shTG) or scrambled shRNA (shRNA) were constructed. Hyperosmolar conditions reduced viability and increased mitochondrial depolarization in shRNA cells. However, hyperosmolarity failed to induce mitochondrial depolarization to the same extent in shTG cells. Transient overexpression of TGM‐2 resulted in very high levels of TGM‐2 expression in shTG and shRNA cells. In the case of shTG cells after overexpression of TGM‐2, hyperosmolarity induced the same extent of mitochondrial depolarization as similarly treated shRNA cells. Overexpression of TGM‐2 also elevated transamidase activity and reduced viability. It also induced mitochondrial depolarization, increased caspase‐3/7 and ‐9 activity, and these increases were partially suppressed by pan‐caspase inhibitor Z‐VAD‐FMK. Corneal epithelial apoptosis via mitochondrial dysfunction after hyperosmolar stimulation is partially dependent on TGM‐2. This TGM‐2‐dependent mechanism occurs in part via caspase‐3/7 and ‐9. Protection against mitochondrial stress in the ocular surface targeting TGM‐2 may have important implications in the survival of cells in hyperosmolar stress. J. Cell. Physiol. 226: 693–699, 2011. © 2010 Wiley‐Liss, Inc.
Wnt‐3a and Wnt‐3 differently stimulate proliferation and neurogenesis of spinal neural precursors and promote neurite outgrowth by canonical signaling
Authors: David Monica D. , Cantí Carles , Herreros Judit 
Published: August 2010

AbstractWnt factors regulate neural stem cell development and neuronal connectivity. Here we investigated whether Wnt‐3a and Wnt‐3, expressed in the developing spinal cord, regulate proliferation and the neuronal differentiation of spinal cord neural precursors (SCNP). Wnt‐3a promoted a sustained increase of SCNP proliferation and decreased the expression of cyclin‐dependent kinase inhibitors. In contrast, Wnt‐3 transiently enhanced SCNP proliferation and increased neurogenesis through β‐catenin signaling. Furthermore, both Wnt‐3a and Wnt‐3 stimulated neurite outgrowth in SCNP‐derived neurons through β‐catenin‐ and TCF4‐dependent transcription. Glycogen synthase kinase‐3β inhibitors mimicked Wnt signaling and promoted neurite outgrowth in established cultures. We conclude that Wnt‐3a and Wnt‐3 factors signal through the canonical Wnt/β‐catenin pathway to regulate different aspects of SCNP development. These findings may be of therapeutic interest for the treatment of neurodegenerative diseases and nerve injury. © 2010 Wiley‐Liss, Inc.
Incidental gallbladder cancer: Analysis of surgical findings and survival
Authors: Butte Jean M. , Waugh Enrique , Meneses Manuel , Parada Hugo , De La Fuente Hernán A. 
Published: August 2010

AbstractBackground and ObjectivesIncidental gallbladder cancer (IGBCA) has risen worldwide and its prognosis depends on complete radical cholecystectomy (CRC). This study evaluated surgical findings during re‐operation and survival of patients with IGBCA.MethodsDemographics, surgical treatment, staging, and survival data for all IGBCA patients who underwent surgery at Instituto Oncológico Fundación Arturo López Pérez (FALP) between 2000 and 2008 were analyzed. Differences between groups were analyzed by Student's t‐test, Mann–Whitney, chi‐square, or Fisher log‐rank tests.ResultsForty‐nine patients were studied (38 women/11 men, median age = 58 years). Pathology reports from cholecystectomy showed that 32 patients had a T2 tumor and 12 had positive resection margin. Thirty‐six patients underwent surgical re‐exploration and 20 underwent CRC; 10 with (+) residual disease and 10 with (−). For patients with at least T1b tumor, median survival was 28 months and 5‐year disease‐specific survival (DSS) was 29%. The 3‐year DSS was 64% for CRC (−), 30% for CRC (+), and 8% for non‐resected cases (P < 0.007). The 3‐year DSS was better for patients with stage Ib than those with stages II and IV (P < 0.007).ConclusionsPatients with IGBCA have a high chance of intra‐abdominal metastases or local residual disease. In CRC patients, intra‐abdominal metastases were associated with a worse prognosis. J. Surg. Oncol. 2010;102:620–625. © 2010 Wiley‐Liss, Inc.
Safety and potential benefit of hyperthermic intraperitoneal chemotherapy (HIPEC) in peritoneal carcinomatosis from primary or recurrent ovarian cancer
Authors: Roviello Franco , Pinto Enrico , Corso Giovanni , Pedrazzani Corrado , Caruso Stefano , Filippeschi Marco , Petrioli Roberto , Marsili Stefania , Mazzei Maria Antonietta , Marrelli Daniele 
Published: August 2010

AbstractObjectivesTo analyze the outcomes of cytoreductive surgery and HIPEC in patients with peritoneal carcinomatosis from ovarian cancer.MethodsFifty‐three patients with peritoneal carcinomatosis from primary (45 cases) and recurrent (8 cases) ovarian cancer were previously treated by systemic chemotherapy with platinum and taxanes and then submitted to surgical cytoreduction and HIPEC (cisplatin and mitomycin‐C) with a closed abdomen technique. The median follow‐up period was 27 months (range: 3–107).ResultsAt the end of operation a complete cytoreduction (CCR‐0) was obtained in 37 patients (70%). Major morbidity occurred in 12 patients (23%); reoperation was necessary in 2 patients (4%), and no postoperative mortality was observed. Overall 5‐year survival probability was 55%; it was 71% in CCR‐0, 44% in CCR‐1, and none in patients with CCR‐2 or CCR‐3 residual tumor (log‐rank test: P = 0.017). The cumulative risk of recurrence in 37 CCR‐0 cases was 54% at 5 years from operation.ConclusionsThe results of our study indicate the feasibility and the potential benefit of a protocol including systemic chemotherapy, surgical cytoreduction and HIPEC in patients with peritoneal carcinomatosis from ovarian cancer. A phase III trial to compare this approach with conventional treatment is needed. J. Surg. Oncol. 2010;102:663–670. © 2010 Wiley‐Liss, Inc.
Subtotal stomach‐preserving pancreaticoduodenectomy (SSPPD) prevents postoperative delayed gastric emptying
Authors: Kurahara Hiroshi , Takao Sonshin , Shinchi Hiroyuki , Mataki Yuko , Maemura Kousei , Sakoda Masahiko , Ueno Shinichi , Natsugoe Shoji 
Published: August 2010

AbstractBackground and ObjectivesDelayed gastric emptying (DGE) is one of main complications after pylorus‐preserving pancreaticoduodenectomy (PPPD) with regional lymph node dissection (RLND). The aim of this study was to retrospectively investigate whether subtotal stomach‐preserving PD (SSPPD) decreased incidence of DGE.MethodsThis study included 112 consecutive patients underwent PPPD (n = 48) or SSPPD (n = 64) with/without RLND. DGE was classified into three categories (grades A, B, and C) according to the guideline proposed by the International Study Group of Pancreatic Surgery.ResultsThe incidence of DGE grade B/C in SSPPD with RLND (13.0%) was lower compared with that (34.8%) in PPPD with RLND (P = 0.0326). Consequently, the mean length of postoperative hospital stay of SSPPD with RLND group was significantly shorter than that of PPPD with RLND (P = 0.0476).ConclusionsSSPPD could be substituted for PPPD due to decreased postoperative DGE when RLND is involved. A randomized control trial of SSPPD versus PPPD should be considered. J. Surg. Oncol. 2010;102:615–619. © 2010 Wiley‐Liss, Inc.
Relation of MT1‐MMP gene expression to outcomes in colorectal cancer
Authors: Kanazawa Amane , Oshima Takashi , Yoshihara Kazue , Tamura Shuzo , Yamada Takanobu , Inagaki Daisuke , Sato Tsutomu , Yamamoto Naoto , Shiozawa Manabu , Morinaga Soichiro , Akaike Makoto , Kunisaki Chikara , Tanaka Katsuaki , Masuda Munetaka , Imada Toshio 
Published: August 2010

AbstractBackgroundMatrix metalloproteinases are members of a large family of endopeptidases that participate in the extracellular‐matrix degradation that accompanies cancer cell invasion, metastasis and angiogenesis. The membrane‐type 1 matrix metalloproteinase (MT1‐MMP) gene has been reported in various cancers and is associated with tumor invasion and metastasis. This study examined the relation of the relative expression of MT1‐MMP gene to clinicopathological factors and outcomes in patients with colorectal cancer (CRC).MethodsWe studied surgical specimens of cancer tissue and adjacent normal mucosa obtained from 202 patients with untreated CRC. The relative expression levels of MT1‐MMP mRNA in cancer and in normal adjacent mucosa were measured by quantitative real‐time reverse‐transcriptase polymerase chain reaction.ResultsMT1‐MMP gene expression was higher in cancer tissue than in adjacent normal mucosa. The level of MT1‐MMP gene expression was not related to any clinicopathological factor. Overall survival at 5 years differed significantly between patients with high MT1‐MMP gene expression and those with low expression.ConclusionsOverexpression of the MT1‐MMP gene is considered a useful independent predictor of outcomes in patients with CRC. J. Surg. Oncol. 2010;102:571–575. © 2010 Wiley‐Liss, Inc.
New techniques to detect unknown primaries in cervical lymph node metastasis
Authors: Sakai Akihiro , Okami Kenji , Ebisumoto Koji , Sugimoto Ryosuke , Maki Daisuke , Iida Masahiro 
Published: August 2010

AbstractObjective:Various methods have been reported for the detection of unknown primaries in cervical lymph node metastasis. Recently, we applied new optical devices and modifications of endoscopic techniques for the detection of primary lesions, and obtained excellent results. The detection rate of the new method was compared with that of previous methods.Study Design:Retrospective.Methods:A total of 51 patients with cervical lymph node metastasis from an unknown primary site (CUP) were referred to our hospital between January 2000 and May 2009, and were retrospectively analyzed. Between 2000 and 2005, the observation by normal video‐endoscope in straight head position was performed to detect the primary lesions in the outpatient setting. Since 2006, a new method for detection of primary lesions has been employed. The method includes the use of new optical devices (hooded video‐endoscope and narrow‐band imaging endoscope) and different head positions (head torsion technique, Valsalva maneuver, and the Killian position).Results:The detection rate of primary lesions using the new method was 71% (15 of 21), which was better than the 30% (10 of 30) obtained with the conventional method. All primary lesions identified using the new method were located in the hypopharynx.Conclusions:The new method was able to detect primary lesions in 71% of cases with CUP. The higher rate achieved with the new method was probably due to the clear visualization of the hypopharynx. Thus, the new method was shown to be useful for the detection of primary lesions in cases of CUP. Laryngoscope, 2010
Evaluation of guinea pig model for ocular and cervical vestibular‐evoked myogenic potentials for vestibular function test
Authors: Yang Ting‐Hua , Liu Shing‐Hwa , Young Yi‐Ho 
Published: August 2010

AbstractObjectives/Hypothesis:This study used air‐conducted sound (ACS) and bone‐conducted vibration (BCV) stimuli in eliciting ocular vestibular‐evoked myogenic potential (oVEMP) and cervical VEMP (cVEMP) in guinea pigs.Study Design:Prospective study.Methods:Ten guinea pigs were treated with gentamicin (4 mg) on the left ear, whereas the right ear served as a control. One week after treatment, each animal underwent oVEMP and cVEMP tests using ACS and BCV modes in a randomized order, and was sacrificed for morphological study.Results:Using ACS mode, oVEMPs were absent in all 10 (100%) animals despite the stimulus intensity increased up to 120 dB pe SPL. Conversely, using BCV mode, oVEMPs were present on the left (lesion) eye, and absent on the right (control) eye in all (100%) animals. For the cVEMPs via ACS mode, all right (control) necks had clear cVEMPs, and all (100%) left (lesion) necks revealed absent cVEMPs. However, via BCV mode, all right (control) necks and six (60%) left (lesion) necks showed clear cVEMPs. Morphological study demonstrated substantial loss of hair cells in the utricular and saccular macula.Conclusions:The cVEMP test via ACS mode is specific for investigating the saccular disorder, whereas the oVEMP test via BCV mode is preferable for investigating the utricular disorders in humans. The guinea pig model is consistent with the findings of humans. Restated, appropriate animal models for cVEMP and oVEMP in guinea pigs are via ACS and BCV modes, respectively. Laryngoscope, 2010
Amplification of CyclinL1 in uterine cervical carcinoma has prognostic implications
Authors: Mitra Sraboni , Mazumder (Indra) Dipanjana , Basu Partha S. , Mondal Ranajit K. , Roy Anup , Roychoudhury Susanta , Panda Chinmay K. 
Published: August 2010

AbstractThe chromosomal 3q25.31 region was consistently amplified in primary cancer of cervix (CACX). CyclinL1 is a candidate gene of this region and already have been implicated as an oncogene in head and neck cancers. In this study, we aimed to investigate the involvement of CyclinL1 in cervical carcinogenesis and for this purpose its copy number variation (CNV) was studied in 23 cervical intraepithelial neoplasia (CIN) and 110 CACX samples. In CIN lesions CyclinL1 was not amplified; however, the amplification frequency was 16% (9/56) in stage I/II tumors which remained comparable during subsequent stages of tumorigenesis. This implied association of CyclinL1 amplification with development of early invasiveness. Quantitation of mRNA expression revealed 2.6 ± 1.53‐fold overexpression of this gene in primary CACX. The amplification/copy number gain of CyclinL1 and its mRNA profile were concordant, in tumors. Immunohistochemical (IHC) analysis in primary CACX, cell lines: SiHa and HeLa revealed intense nuclear expression of cyclinL1, which was further confirmed by Western blot in the cell lines. However 47% (7/15) CACX samples expressed high/intermediate level of cyclin L1. Kaplan–Meier survival analysis indicated CyclinL1 amplification as a determinant of poor patient outcome. Tumor radio‐resistance developed as a consequence of CyclinL1 amplification. Cox multivariate analysis revealed that multiparous (≥5) CACX patients with amplified CyclinL1 locus along with advanced tumor stage (III/IV) had worst prognosis. Our data suggest importance of CyclinL1 in cervical carcinogenesis with its associated pathways viz: pre‐mRNA splicing, cell‐cycle regulation (G0/G1 and G2/M) being potential targets of therapeutic interventions in CACX. © 2010 Wiley‐Liss, Inc.
Association of a common AGO1 variant with lung cancer risk: A two‐stage case–control study
Authors: Kim Jong‐Sik , Choi Yi Young , Jin Guang , Kang Hyo‐Gyoung , Choi Jin‐Eun , Jeon Hyo‐Sung , Lee Won‐Kee , Kim Dong‐Sun , Kim Chang Ho , Kim Young Jin , Son Ji‐Woong , Jung Tae Hoon , Park Jae Yong 
Published: August 2010

AbstractBased on the important role of microRNA (miRNA) biosynthesis genes in carcinogenesis, we hypothesized that polymorphisms in the miRNA biosynthesis genes may modulate susceptibility to lung cancer. To test this hypothesis, we conducted a two‐stage study to evaluate the associations between single nucleotide polymorphisms (SNPs) in the miRNA biosynthesis genes and the risk of lung cancer. In stage 1 of the study, 24 SNPs in the 11 miRNA biosynthesis genes (DROSHA, DGCR8, RAN, XPO5, DICER, AGO1, AGO2, HIWI, GEMIN3, GEMIN4, and TRBP) were genotyped in 100 lung cancer patients and 100 healthy controls using a sequenome mass spectrometry‐based genotyping assay. One promising SNP (AGO1 rs636832A > G) was selected for stage 2 of the study, and genotyped by a melting‐curve analysis using fluorescence‐labeled hybridization probes in an independent set of 552 cases and 552 controls. The AGO1 rs636832A > G exhibited highly consistent results between the two stages of the study. In combined analysis, the 636832A > G was associated with a significantly decreased risk of lung cancer in a dose‐dependent manner (Ptrend = 6.0 × 10−4). Individuals with at least one rs636832G allele were at a significantly decreased risk of lung cancer compared with those with the AA genotype (adjusted odds ratio = 0.67, 95% confidence interval = 0.53–0.84, P = 4.0 × 10−4). This finding suggests that the AGO1 rs636832A > G might be a useful marker for determining the susceptibility to lung cancer and that the AGO1 gene might be involved in the development of lung cancer. © 2010 Wiley‐Liss, Inc.
Impaired sense of smell and color discrimination in monogenic and idiopathic Parkinson's disease
Authors: Kertelge Lena , Brüggemann Norbert , Schmidt Alexander , Tadic Vera , Wisse Claudia , Dankert Sylwia , Drude Laura , van der Vegt Joyce , Siebner Hartwig , Pawlack Heike , Pramstaller Peter P. , Behrens Maria Isabel , Ramirez Alfredo , Reichel Dirk , Buhmann Carsten , Hagenah Johann , Klein Christine , Lohmann Katja , Kasten Meike 
Published: August 2010

AbstractOlfaction is typically impaired in idiopathic Parkinson's disease (IPD), but its role is uncertain in monogenic PD. Diminished color discrimination has been suggested as another early sign of dopaminergic dysfunction but not been systematically studied. Furthermore, it is unknown whether both deficits are linked. We examined 100 patients with IPD, 27 manifesting mutation carriers (MC), 20 nonmanifesting mutation carriers (NMC), and 110 controls. Participants underwent a standardized neurological examination, the University of Pennsylvania Smell Identification Test (UPSIT), the Farnsworth‐Munsell (FM) color discrimination test, and mutation testing in known PD genes. The monogenic group consisted of 15 Parkin (6MC/9NMC), 17 PINK1 (10MC/7NMC), 8 LRRK2 (4MC/4NMC), 3 SNCA (MC), and 4 ATP13A2 (MC) carriers. Olfaction was most impaired in IPD (UPSIT percentiles 10.1 ± 13.5) compared with all other groups (MC 13.8 ± 11.9, NMC 19.6 ± 13.0, controls 33.8 ± 22.4). Within MC, carriers of two mutations in Parkin and PINK1 showed higher UPSIT percentiles than LRRK2 and SNCA carriers. Color discrimination was reduced in IPD (FM total error score 134.8 ± 92.7). In MC (122.4 ± 142.4), the reduction was most pronounced in LRRK2, NMC (80.0 ± 38.8) were comparable with controls (97.2 ± 61.1). UPSIT and FM scores were correlated in the control (r = −0.305; P = 0.002) and the IPD group (r = −0.303; P = 0.006) but not among mutation carriers. First, we confirmed olfaction and color discrimination to be impaired in IPD and suggest olfaction to be a premotor sign. Second, olfaction differed between carriers with one and two mutations in Parkin/PINK1‐associated PD. Third, olfaction and color discrimination impairment do not necessarily evolve in parallel. © 2010 Movement Disorder Society
Comprehensive sequencing of the LRRK2 gene in patients with familial Parkinson's disease from North Africa
Authors: Jasinska‐Myga Barbara , Kachergus Jennifer , Vilariño‐Güell Carles , Wider Christian , Soto‐Ortolaza Alexandra I. , Kefi Mounir , Middleton Lefkos T. , Ishihara‐Paul Lianna , Gibson Rachel A. , Amouri Rim , Yahmed Samia Ben , Sassi Samia Ben , Zouari Mourad , Euch Ghada El , Ross Owen A. , Hentati Faycal , Farrer Matthew J. 
Published: August 2010

AbstractThe LRRK2 gene is a key player in Parkinson's disease (PD), however prevalence and pathogenicity of LRRK2 variants remain to be investigated in ethnically diverse populations. Herein, we performed comprehensive sequencing of the LRRK2 gene in 92 Tunisian probands with familial PD. We then performed an association study using all identified variants in a series of 167 Lrrk2 p.G2019S‐negative patients with sporadic PD and 365 Lrrk2 p.G2019S‐negative healthy control subjects, all from the same Arab‐Berber ethnicity. We identified one novel coding substitution (p.M2408I) and 24 known coding changes. Only the Lrrk2 p.G2019S mutation segregated with disease within families and was found in 39% of familial probands. None of the variants displayed significant association with risk for sporadic PD, however a trend was observed for Lrrk2 p.Y2189C. The present study underscores the importance of the LRRK2 gene in the Tunisian PD population. © 2010 Movement Disorder Society
Novel planar triple band monopole antenna for WiMAX/WLAN applications
Authors: Liu Hsien‐Wen , Ku Chia‐Hao 
Published: August 2010

AbstractA coplanar waveguide (CPW)‐fed planar monopole antenna with triple band operation for worldwide interoperability for microwave access (WiMAX) and wireless local area network (WLAN) applications is presented. The antenna, which occupies a small size of 25 (L) × 25 (W) × 0.8 (H) mm3, is simply composed of a pentagonal radiating patch with two bent slots. By carefully selecting the positions and lengths of these slots, good dual stopband rejection characteristic of the antenna can be obtained, so that three operating bands covering 2.14–2.85, 3.29–4.08, and 5.02–6.09 GHz can be achieved. The measured results also demonstrate that the proposed antenna has good omnidirectional radiation patterns with appreciable gain across the operating bands, and is thus suitable to be integrated within the portable devices for WiMAX/WLAN applications. © 2010 Wiley Periodicals, Inc. Microwave Opt Technol Lett 52:2405–2408, 2010; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.25497