bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discovery, Identification and Comparative Analysis of Non-Specific Lipid Transfer Protein (nsLtp) Family in Solanaceae
Authors: Liu Wanfei , Huang Dawei , Liu Kan , Hu Songnian , Yu Jun , Gao Gang , Song Shuhui 
Published: March 2011

Abstract
Plant non-specific lipid transfer proteins (nsLtps) have been reported to be involved in plant defense activity against bacterial and fungal pathogens. In this study, we identified 135 (122 putative and 13 previously identified) Solanaceae nsLtps, which are clustered into 8 different groups. By comparing with Boutrot’s nsLtp classification, we classified these eight groups into five types (I, II, IV, IX and X). We compared Solanaceae nsLtps with Arabidopsis and Gramineae nsLtps and found that (1) Types I, II and IV are shared by Solanaceae, Gramineae and Arabidopsis; (2) Types III, V, VI and VIII are shared by Gramineae and Arabidopsis but not detected in Solanaceae so far; (3) Type VII is only found in Gramineae whereas type IX is present only in Arabidopsis and Solanaceae; (4) Type X is a new type that accounts for 52.59% Solanaceae nsLtps in our data, and has not been reported in any other plant so far. We further built and compared the three-dimensional structures of the eight groups, and found that the major functional diversification within the nsLtp family could be predated to the monocot/dicot divergence, and many gene duplications and sequence variations had happened in the nsLtp family after the monocot/dicot divergence, especially in Solanaceae.
A Multifunctional Lentiviral-Based Gene Knockdown with Concurrent Rescue that Controls for Off-Target Effects of RNAi
Authors: Feng Yunfeng , Nie Linghu , Thakur Meghna Das , Su Qin , Chi Zhenfen , Zhao Yongliang , Longmore Gregory D. 
Published: March 2011

Abstract
The efficient, stable delivery of siRNA into cells, and the appropriate controls for non-specific off-target effects of siRNA are major limitations to functional studies using siRNA technology. To overcome these drawbacks, we have developed a single lentiviral vector that can concurrently deplete endogenous gene expression while expressing an epitope-tagged siRNA-resistant target gene in the same cell. To demonstrate the functional utility of this system, we performed RNAi-depleted α-actinin-1 (α-ACTN1) expression in human T cells. α-ACTN1 RNAi resulted in inhibited chemotaxis to SDF-1α, but it can be completely rescued by concurrent expression of RNAi-resistant α-ACTN1 (rr-α-ACTN1) in the same cell. The presence of a GFP tag on rr-α-ACTN1 allowed for detection of appropriate subcellular localization of rr-α-ACTN1. This system provides not only an internal control for RNAi off-target effects, but also the potential tool for rapid structure-function analyses and gene therapy.
Similarity scaling and vorticity structure in high-Reynolds-number stably stratified turbulent wakes
Authors: DIAMESSIS PETER J. , SPEDDING GEOFFREY R. , DOMARADZKI J. ANDRZEJ 
Published: March 2011

The mean velocity profile scaling and the vorticity structure of a stably stratified, initially turbulent wake of a towed sphere are studied numerically using a high-accuracy spectral multi-domain penalty method model. A detailed initialization procedure allows a smooth, minimum-transient transition into the non-equilibrium (NEQ) regime of wake evolution. A broad range of Reynolds numbers,Re= UD/ν ∈ [5 × 103, 105] and internal Froude numbers,Fr= 2U/(ND) ∈ [4, 64] (U,Dare characteristic velocity and length scales, andNis the buoyancy frequency) is examined. The maximum value ofReand the range ofFrvalues considered allow extrapolation of the results to geophysical and naval applications.At higherRe, the NEQ regime, where three-dimensional turbulence adjusts towards a quasi-two-dimensional, buoyancy-dominated flow, lasts significantly longer than at lowerRe. AtRe= 5 × 103, vertical fluid motions are rapidly suppressed, but atRe= 105, secondary Kelvin–Helmholtz instabilities and ensuing turbulence are clearly observed up toNt≈ 100. The secondary motions intensify with increasing stratification strength and have significant vertical kinetic energy.These results agree with existing scaling of buoyancy-driven shear onRe/Fr2and suggest that, in the field, the NEQ regime may last up toNt≈ 1000. At a given highRevalue, during the NEQ regime, the scale separation between Ozmidov and Kolmogorov scale is independent ofFr. This first systematic numerical investigation of stratified turbulence (as defined by Lilly,J. Atmos. Sci.vol. 40, 1983, p. 749), in a controlled localized flow with turbulent initial conditions suggests that a reconsideration of the commonly perceived life cycle of a stratified turbulent event may be in order for the correct turbulence parametrizations of such flows in both geophysical and operational contexts.
Editorial
Authors: Cairns Alan , Infeld Eryk 
Published: March 2011

This special issue marks the 65th birthday of John Dougherty, the founding
father of this Journal and its Editor for the first 27 years of its life. Since the
Journal is so much John's creation, we thought that it would not be appropriate
to let this occasion pass without recognition, and we are pleased that a number
of John's former students and a selection of people who have contributed
regularly to the Journal or have been Associate Editors have agreed to
contribute to this issue.Most plasma physicists will probably associate John with at least three major
contributions to the subject. The first is his classic work with Don Farley on
scattering of electromagnetic waves from a plasma, and we are very pleased
that Don has written a short note about the genesis of this work and about his
collaboration with John. The second is his book on plasma physics written with
P. C. Clemmow, which, some 30 years after its publication, is still one of the best
available texts on some advanced aspects of the subject. It received the rare
accolade of being reprinted in the Addison-Wesley Advanced Book Classics
series 21 years after its first publication. Finally, there is his editorship of this
Journal, which he continued long after his own interests had shifted from
plasma physics to fundamental aspects of statistical mechanics. Under John's
stewardship, the Journal published papers on all aspects of plasma physics, but
perhaps became specially noted for thoughtful contributions to basic aspects of
the subject.All readers who know John, whether as authors, visitors to Cambridge or in
any other capacity, will be aware of his friendliness and willingness to help
others. On behalf of the plasma physics community, we would like to pass on
to him our appreciation of his contribution, our congratulations on the occasion
of his 65th birthday and our best wishes for the future.
Do adult emotional and behavioural outcomes vary as a function of diverse childhood experiences of the public care system?
Authors: Dregan A. , Brown J. , Armstrong D. 
Published: March 2011

BackgroundLongitudinal data from the 1970 British Cohort Study were used to examine the long-term adult outcomes of those who, as children, were placed in public care.MethodMultivariate logistic estimation models were used to determine whether public care and placement patterns were associated with adult psychosocial outcomes. Seven emotional and behavioural outcomes measured at age 30 years were considered: depression, life dissatisfaction, self-efficacy, alcohol problems, smoking, drug abuse, and criminal convictions.ResultsThe analyses revealed a significant association between public care status and adult maladjustment on depression [odds ratio (OR) 1.74], life dissatisfaction (OR 1.45), low self-efficacy (OR 1.95), smoking (OR 1.70) and criminal convictions (OR 2.13).ConclusionsOverall, the present study findings suggest that there are enduring influences of a childhood admission to public care on emotional and behavioural adjustment from birth to adulthood. Some of the associations with childhood public care were relatively strong, particularly with respect to depression, self-efficacy and criminal convictions.
‘A blow to the men in Pink’: The Royal Society for the Prevention of Cruelty to Animals and Opposition to Hunting in the Twentieth Century
Authors: TICHELAR MICHAEL 
Published: March 2011

AbstractIn 1976 the RSPCA finally adopted a policy of opposition to fox hunting and the shooting of birds for sport after a long history of highly controversial internal debate and external lobbying by pressure groups. This article explores the possible long-term historical reasons behind the change of policy. It seeks to begin to fill a gap in the historical literature and explain the changes in public opinion towards hunting that occurred during the course of the twentieth century by looking at key episodes in the history of the RSPCA up to the 1970s. It shows there was a decline in aristocratic dominance on the ruling council of the RSPCA after the 1920s, but other reasons for the change in policy included the increasing influence of an urban/metropolitan view of the countryside after the 1950s; changing public attitudes towards farmers, who had been traditional supporters of hunting; and the increasing importance of environmentalism and ecology after 1960. By the end of the twentieth century it was no longer possible to argue that hunting was an essential feature of rural society and culture, despite the continuing popularity and survival of fox hunting with hounds.
Will, Obligatory Ends and the Completion of Practical Reason: Comments on Barbara Herman'sMoral Literacy
Authors: Reath Andrews 
Published: March 2011

AbstractThis paper discusses three inter-related themes in Barbara Herman'sMoral Literacy– the idea that, for Kant, the will is a ‘norm-constituted power’ whose activity is guided by its own internal norm, that the obligatory ends are reasonably viewed as the ends of all rational choice, and that morality ‘completes’ practical reason or rational agency.
A New Kantian Response to Maxim-Fiddling
Authors: Sneddon Andrew 
Published: March 2011

AbstractThere has long been a suspicion that Kant's test for the universalizability of maxims can be easily subverted: instead of risking failing the test, design your maxim for any action whatsoever in a manner guaranteed to pass. This is the problem of maxim-fiddling. The present discussion of this problem has two theses:1]That extant approaches to maxim-fiddling are not satisfactory;2]That a satisfactory response to maxim-fiddling can be articulated using Kantian resources, especially the first two formulations of the categorical imperative.This approach to maxim-fiddling draws our attention to a Kantian notion of an offence against morality itself that has largely been overlooked.
Membrane nanotubes induced by aqueous phase separation and stabilized by spontaneous curvature
Authors: Li Yanhong , Lipowsky Reinhard , Dimova Rumiana 
Published: March 2011

Tubular membrane structures are widespread in eukaryotic cells, but the mechanisms underlying their formation and stability are not well understood. Previous work has focused on tube extrusion from cells and model membranes under the application of external forces. Here, we present novel membrane/polymer systems, where stable tubes form in the absence of externally applied forces. Solutions of two water-soluble polymers, polyethylene glycol and dextran, were encapsulated in giant lipid vesicles, cell-size model systems. Hypertonic deflation induced phase separation of the enclosed solution. The excess membrane area created during the deflation process was stored in a large number of membrane nanotubes inside the vesicle. The tubes had a diameter below optical resolution and became visible only when fluorescently labeled. The tubes were rather stable: In the absence of external forces, they existed for several days. A theoretical analysis of the shapes of the deflated vesicles reveals that these shapes would be unstable if the membranes had no spontaneous curvature. Using the large separation of length scales between the tube diameter and the overall size of the vesicles, the spontaneous curvature can be calculated and is found to be about -1/(240 nm) for a certain range of polymer concentrations. The nanotubes could also be retracted back into the mother vesicle by increasing the membrane tension via micropipette aspiration of the vesicle. Membrane tubes, which can form and be retracted easily, should be relevant for lipid storage in cells.
The Network Approach for Prevention of Healthcare-Associated Infections: Long-Term Effect of Participation in the Duke Infection Control Outreach Network
Authors: Anderson Deverick J. , Miller Becky A. , Chen Luke F. , Adcock Linda H. , Cook Evelyn , Cromer A. Lynn , Louis Susan , Thacker Paul A. , Sexton Daniel J. 
Published: March 2011

Objective.To describe the rates of several key outcomes and healthcare-associated infections (HAIs) among hospitals that participated in the Duke Infection Control Outreach Network (DICON).Design and Setting.Prospective, observational cohort study of patients admitted to 24 community hospitals from 2003 through 2009.Methods.The following data were collected and analyzed: incidence of central line-associated bloodstream infections (CLABSIs), ventilator-associated pneumonia (VAP), catheter-associated urinary tract infections (CAUTIs), and HAIs caused by methicillin-resistant Staphylococcus aureus (MRSA); employee exposures to bloodborne pathogens (EBBPs); physician EBBPs; patient-days; central line-days; ventilator-days; and urinary catheter-days. Poisson regression was used to determine whether incidence rates of these HAIs and exposures changed during the first 5 and 7 years of participation in DICON; nonrandom clustering of each outcome was controlled for. Cost saved and lives saved were calculated on the basis of published estimates.Results.In total, we analyzed 6.5 million patient-days, 4,783 EBPPs, 2,948 HAIs due to MRSA, and 2,076 device-related infections. Rates of employee EBBPs, HAIs due to MRSA, and device-related infections decreased significantly during the first 5 years of participation in DICON (P < .05 for all models; average decrease was approximately 50%); in contrast, physician EBBPs remained unchanged. In aggregate, 210 CLABSIs, 312 cases of VAP, 332 CAUTIs, 1,042 HAIs due to MRSA, and 1,016 employee EBBPs were prevented. Each hospital saved approximately $100,000 per year of participation, and collectively the hospitals may have prevented 52-105 deaths from CLABSI or VAP. The 7-year analysis demonstrated that these trends continued with further participation.Conclusions.Hospitals with long-term participation in an infection control network decreased rates of significant HAIs by approximately 50%, decreased costs, and saved lives.
Development and Validation of a Clostridium difficile Infection Risk Prediction Model
Authors: Dubberke Erik R. , Yan Yan , Reske Kimberly A. , Butler Anne M. , Doherty Joshua , Pham Victor , Fraser Victoria J. 
Published: March 2011

Objective.To develop and validate a risk prediction model that could identify patients at high risk for Clostridium difficile infection (CDI) before they develop disease.Design and Setting.Retrospective cohort study in a tertiary care medical center.Patients.Patients admitted to the hospital for at least 48 hours during the calendar year 2003.Methods.Data were collected electronically from the hospital's Medical Informatics database and analyzed with logistic regression to determine variables that best predicted patients' risk for development of CDI. Model discrimination and calibration were calculated. The model was bootstrapped 500 times to validate the predictive accuracy. A receiver operating characteristic curve was calculated to evaluate potential risk cutoffs.Results.A total of 35,350 admitted patients, including 329 with CDI, were studied. Variables in the risk prediction model were age, CDI pressure, times admitted to hospital in the previous 60 days, modified Acute Physiology Score, days of treatment with high-risk antibiotics, whether albumin level was low, admission to an intensive care unit, and receipt of laxatives, gastric acid suppressors, or antimotility drugs. The calibration and discrimination of the model were very good to excellent (C index, 0.88; Brier score, 0.009).Conclusions.The CDI risk prediction model performed well. Further study is needed to determine whether it could be used in a clinical setting to prevent CDI-associated outcomes and reduce costs.
Championing Patient Safety through Mandatory Influenza Vaccination for All Healthcare Personnel and Affiliated Physicians
Authors: Karanfil Lynne V. , Bahner Jan , Hovatter Joan , Thomas William L. 
Published: March 2011

Objective.To determine whether a mandatory seasonal influenza vaccination program will increase vaccination rates among healthcare personnel (HCP) and affiliated physicians.Setting.MedStar Health is a not-for-profit regional healthcare organization that includes 9 hospitals with approximately 25,000 HCP and approximately 4,000 affiliated physicians. HCP describes any person employed by MedStar Health.Methods.With previous vaccination rates parallel to reported national rates of 54% among HCP, MedStar Health introduced a mandatory seasonal influenza vaccination program promulgated during the 2009–2010 influenza season. HCP and affiliated physicians were given an opportunity to apply for medical or religious exemptions. Noncompliant HCP were terminated. Noncompliant physicians had their privileges administratively suspended for the influenza season.Results.HCP compliance (vaccinated and exempt) was 99.9%. The influenza vaccination rate among HCP was 98.5%. There were 338 approved medical exemptions and 18 approved religious exemptions. Only 0.01% of HCP (9 full-time, 2 part-time, and 17 per diem employees) were terminated. Overall, 93% of the affiliated physicians were vaccinated; 7 religious and 99 medical exemptions were granted. In total, 149 physicians (4%) had their admitting privileges suspended during the influenza season.Conclusion.A mandatory influenza vaccination program achieves high rates of vaccination among HCP and affiliated physicians.
Use of Phytases in Ethanol Production from E‐Mill Corn Processing
Authors: Khullar Esha , Shetty Jay K. , Rausch Kent D. , Tumbleson M. E. , Singh Vijay 
Published: March 2011

ABSTRACTEffects of phytase addition, germ, and pericarp fiber recovery were evaluated for the E‐Mill dry grind corn process. In the E‐Mill process, corn was soaked in water followed by incubation with starch hydrolyzing enzymes. For each phytase treatment, an additional phytase incubation step was performed before incubation with starch hydrolyzing enzymes. Germ and pericarp fiber were recovered after incubation with starch hydrolyzing enzymes. Preliminary studies on phytase addition resulted in germ with higher oil (40.9%), protein (20.0%), and lower residual starch (12.2%) contents compared to oil (39.1%), protein (19.2%), and starch (18.1%) in germ from the E‐Mill process without phytase addition. Phytase treatment resulted in lower residual starch contents in pericarp fiber (19.9%) compared to pericarp fiber without phytase addition (27.4%). Results obtained led to further investigation of effects of phytase on final ethanol concentrations, germ, pericarp fiber, and DDGS recovery. Final ethanol concentrations were higher in E‐Mill processing with phytase addition (17.4% v/v) than without addition of phytase (16.6% v/v). Incubation with phytases resulted in germ with 4.3% higher oil and 2.5% lower residual starch content compared to control process. Phytase treatment also resulted in lower residual starch and higher protein contents (6.58 and 36.5%, respectively) in DDGS compared to DDGS without phytase incubations (8.14 and 34.2%, respectively). Phytase incubation in E‐Mill processing may assist in increasing coproduct values as well as lead to increased ethanol concentrations.
Drag reduction by riblets
Authors: García-Mayoral Ricardo , Jiménez Javier 
Published: March 2011

The interaction of the overlying turbulent flow with riblets, and its impact on their drag reduction properties are analysed. In the so-called viscous regime of vanishing riblet spacing, the drag reduction is proportional to the riblet size, but for larger riblets the proportionality breaks down, and the drag reduction eventually becomes an increase. It is found that the groove cross section
A
+
g
is a better characterization of this breakdown than the riblet spacing, with an optimum

. It is also found that the breakdown is not associated with the lodging of quasi-streamwise vortices inside the riblet grooves, or with the inapplicability of the Stokes hypothesis to the flow along the grooves, but with the appearance of quasi-two-dimensional spanwise vortices below
y
+
≈30, with typical streamwise wavelengths

. They are connected with a Kelvin–Helmholtz-like instability of the mean velocity profile, also found in flows over plant canopies and other surfaces with transpiration. A simplified stability model for the ribbed surface approximately accounts for the scaling of the viscous breakdown with
A
+
g
.
Addiction Research Centres and the Nurturing of Creativity. National Institute on Alcohol and Drugs Policies, Brazil
Authors: Laranjeira Ronaldo , Mitsuhiro Sandro Sendin 
Published: March 2011

ABSTRACTThe National Institute of Public Policy for Alcohol and Other Drugs (INPAD) is based at the Federal University of São Paulo, Brazil, and was created to collect scientific evidence regarding epidemiology, develop new therapeutic approaches, study health economics and provide education to subsidize the proper measures to change the Brazilian scenario of alcohol and drug consumption. Policies directed towards the control of alcohol and drugs in Brazil are fragmented, poorly enforced and therefore ineffective. The unregulated market of alcohol in Brazil has contributed to the worsening health of the Brazilian population. Since 1994, INPAD has participated actively in academic debates and discussions about alcohol and drug policies and their effects on the political welfare of the country. Many scientific papers and books have been published on this subject, and the internet and other media have provided excellent opportunities for the dissemination of specialized information to the general population.