bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of Meloidogyne arenaria, M. javanica, and M. incognita reaction of the USA Arachis pintoi (Krapov. & W.C. Gregory) germplasm collection
Authors: Carvalho Marcelo Ayres , Quesenberry Kenneth H. 
Published: October 2009

Abstract
Arachis pintoi Krapov. & W.C. Gregory is a herbaceous, perennial legume, exclusively native to Brazil. It is considered a multiple use legume, being grown for forage; ground cover in fruits orchards, forest, and low tillage systems; erosion control; and ornamental purposes. Accessions of the A. pintoi USA germplasm collection of the National Plant Germplasm System (NPGS) were evaluated to characterize its reaction to Meloidogyne arenaria (Neal) Chitwood, M. javanica (Treub) Chitwood, and M. incognita (Kofoid and White) Chitwood. Arachis pintoi germplasm presented great variability and high levels of resistance to M. arenaria, M. javanica, and M. incognita.
Synthesis, X‐ray powder structure analysis and biological properties of a mononuclear Cu(II) complex of N‐2‐hydroxyhippuric acid
Authors: Basu Soumya , Chattopadhyay Basab , Ganguly A. , Chakraborty P. , Roy Chowdhury P. , Samanta S. , Mukherjee M. , Mukherjee A. K. , Choudhuri S. K. 
Published: October 2009

AbstractA mononuclear copper (II) complex of N‐2‐hydroxyhippuric acid (2HHA), [Cu(HA)(H2O)2], has been synthesized and characterized by spectroscopic and X‐ray powder diffraction studies. Crystal structure of [Cu(HA)(H2O)2] reveals a distorted square‐pyramidal geometry around the metal center. The crystal packing in the complex exhibits a three‐dimensional framework formed by intermolecular O; H···O and CH···O hydrogen bonds. Toxicity and antitumor properties of the complex have been studied in vivo. The complex, capable of depleting glutathione (GSH) at nontoxic doses, may be utilized to sensitize drug‐resistant cells where resistance is due to an elevated level of GSH. Copyright © 2009 John Wiley & Sons, Ltd.
Proteomic analysis of novel Cry1Ac binding proteins in Helicoverpa armigera (Hübner)
Authors: Chen Li‐Zhen , Liang Ge‐Mei , Zhang Jie , Wu Kong‐Ming , Guo Yu‐Yuan , Rector Brian G. 
Published: October 2009

AbstractAminopeptidase N (APN) and cadherin‐like proteins have been previously identified as Cry1Ac‐binding proteins in Helicoverpa armigera (Hübner). In this study, a proteomic approach was used to identify novel Cry1Ac‐binding proteins in H. armigera. Brush border membrane vesicles (BBMV) of H. armigera were extracted and separated by two‐dimensional gel electrophoresis (2‐DE). Cry1Ac‐binding proteins were detected using antisera against Cry1Ac. Peptide mass fingerprinting (PMF) was used to identify Cry1Ac‐binding proteins. In total, four proteins were identified as candidate Cry1Ac‐binding proteins in H. armigera: vacuolar ATP synthase (V‐ATPase) subunit B, actin, heat shock cognate protein (HSCP), and a novel protein. © 2009 Wiley Periodicals, Inc.
Measuring change in professionalism attitudes during the gross anatomy course
Authors: Pearson William G. , Hoagland Todd M. 
Published: October 2009

AbstractBy design or default, anatomy educators are often responsible for introducing students to medical professionalism. Although much has been said about the role of anatomical education, there are no published reports suggesting how to measure change. This study investigated what professionalism attitudes, if any, change during a gross anatomy course. Additionally, the influence of four dichotomous variables related to student identity and preparation for medical school were analyzed for their effect on professionalism attitudes. A cross‐sectional time‐one (T1; beginning of the course), time‐two (T2; end of the course) study using the Penn State College of Medicine Survey of Professionalism was conducted. A multivariate analysis of variance identified the main effects and interaction effects of categorical variables. A Mann Whitney U test verified significant differences. This study found a reprioritization of professionalism attitudes in favor of altruism (P = 0.04 with a Cohen's d = 0.26) at T2. Female students (P = 0.03, Cohen's d = 0.38) and students from a science background (P = 0.04, Cohen's d = 0.36) changed the most in favor of altruism. Interestingly, though several factors correlated with dissimilarities in professionalism values at T1, gender was the only factor to show a significant difference in professionalism attitudes at T2. This cohort of students reported a statistically significant increase in altruism and no significant decreases in other professionalism attitudes concurrent with the gross anatomy course. Anat Sci Educ 3:12–16, 2010. © 2009 American Association of Anatomists
Haemoglobin A1c as a predictor of postoperative hyperglycaemia and complications after major colorectal surgery
Authors: Gustafsson U O , Thorell A , Soop M , Ljungqvist O , Nygren J 
Published: October 2009

Abstract

Background
Hyperglycaemia following major surgery increases morbidity, but may be improved by use of enhanced-recovery protocols. It is not known whether preoperative haemoglobin (Hb) A1c could predict hyperglycaemia and/or adverse outcome after colorectal surgery.

Methods
Some 120 patients without known diabetes underwent major colorectal surgery within an enhanced-recovery protocol. HbA1c was measured at admission and 4 weeks after surgery. All patients received an oral diet beginning 4 h after operation. Plasma glucose was monitored five times daily. Patients were stratified according to preoperative levels of HbA1c (within normal range of 4·5–6·0 per cent, or higher).

Results
Thirty-one patients (25·8 per cent) had a preoperative HbA1c level over 6·0 per cent. These had higher mean(s.d.) postoperative glucose (9·3(1·5) versus 8·0(1·5) mmol/l; P &lt; 0·001) and C-reactive protein (137(65) versus 101(52) mg/l; P = 0·008) levels than patients with a normal HbA1c level. Postoperative complications were more common in patients with a high HbA1c level (odds ratio 2·9 (95 per cent confidence interval 1·1 to 7·9)).

Conclusion
Postoperative hyperglycaemia is common among patients with no history of diabetes, even within an enhanced-recovery protocol. Preoperative measurement of HbA1c may identify patients at higher risk of poor glycaemic control and postoperative complications.
Preoperative localization of sentinel lymph nodes using intradermal microbubbles and contrast-enhanced ultrasonography in patients with breast cancer
Authors: Sever A , Jones S , Cox K , Weeks J , Mills P , Jones P 
Published: October 2009

Abstract

Background
Sentinel lymph node (SLN) biopsy is the standard procedure for axillary staging in early breast cancer. Lymphatic imaging after intraparenchymal microbubble injection has been reported in animal models. The aim of this study was to identify and localize SLNs before surgery by contrast-enhanced ultrasonography after intradermal injection of microbubbles in patients with breast cancer.

Methods
Fifty-four consecutive, consenting patients with primary breast cancer were recruited. Patients received a periareolar intradermal injection of microbubble contrast agent. Breast lymphatics were visualized by ultrasonography and followed to identify putative axillary SLNs. A guidewire was deployed to localize the SLN. The next day, patients underwent standard tumour excision and SLN biopsy.

Results
SLNs were identified and guidewires inserted in 48 patients. In these patients operative findings confirmed that the wired lymph nodes were SLNs. The sensitivity of SLN detection, compared with radioisotope and blue dye, was 89 per cent. Five patients were found to have metastases in SLNs. In these patients the SLNs were identified correctly and localized before surgery with guidewires.

Conclusion
SLNs may be identified and localized before surgery using contrast-enhanced ultrasonography after injection of microbubbles.
Hospital volume influences the choice of operation for thyroid cancer
Authors: Lifante J C , Duclos A , Couray-Targe S , Colin C , Peix J L , Schott A M 
Published: October 2009

Abstract

Background
Many authors advocate total or near-total thyroidectomy for thyroid carcinoma. This study examined the relationship between hospital volume of thyroidectomies and choice of bilateral thyroidectomy for thyroid carcinoma.

Methods
Data were extracted from the administrative databases of all hospital discharge abstracts in the Rhône-Alpes area of France. The study population included inpatient stays from 1999 to 2004 with a diagnosis of thyroid disease (benign or malignant) and a procedural code for thyroid surgery. Multivariable logistic regression analyses were performed to determine factors associated with the extent of surgery (unilateral versus bilateral) for thyroid carcinoma.

Results
A total of 20 140 thyroidectomies were identified, including 4006 procedures for cancer. Compared with hospitals performing a high volume of procedures for all thyroid diseases (at least 100 annually), the risk of a unilateral procedure for thyroid cancer increased by 2·46 (95 per cent confidence interval 1·63 to 3·71) in low-volume hospitals (fewer than ten operations per year) and by 1·56 (1·27 to 1·92) in medium-volume centres (ten to 99 per year).

Conclusion
There is a significant relationship between hospital volume and the decision to perform bilateral surgery for thyroid carcinoma. Thyroid cancer surgery should be performed by experienced surgical teams in high-volume centres.
One‐Pot Mechanosynthesis with Three Levels of Molecular Self‐Assembly: Coordination Bonds, Hydrogen Bonds and Host–Guest Inclusion
Authors: Friščić Tomislav , Meštrović Ernest , Škalec Šamec Dijana , Kaitner Branko , Fábián László 
Published: October 2009

AbstractLiquid‐assisted grinding (LAG) was used to combine three levels of molecular self‐assembly into a one‐pot mechanochemical approach for the construction of metal–organic materials. The approach was applied for the construction of three adducts of cobalt(II) dibenzoylmethanate with isonicotinamide, nicotinamide and imidazole, to screen for their inclusion compounds. The one‐pot process consists of: i) The coordination‐driven binding of addends to the equatorially‐protected metal ion, resulting in “wheel‐and‐axle”‐shaped complexes; ii) self‐assembly of resulting complexes by way of hydrogen‐bonded synthons to form metal–organic inclusion hosts; iii) in situ inclusion of the grinding liquid in the resulting host. This approach provided quantitatively and within 20 min the known inclusion compounds of the bis(isonicotinamide) adduct in a single synthetic step. Changing the liquid phase in LAG was used to explore the inclusion behaviour of new wheel‐and‐axle adducts with nicotinamide and imidazole, revealing several inclusion compounds, as well as two polymorphs, of the bis(nicotinamide) host. Preliminary results suggest that one‐pot LAG is superior to solution synthesis in screening for metal–organic inclusion compounds. The difference between the methods is rationalised in terms of reactant solubility and solvent competition. In contrast to the nicotinamide adduct, the bis(imidazole) adduct did not form inclusion compounds. The difference in the inclusion properties of the two adducts is rationalised by structural information gathered by single crystal and powder X‐ray diffraction.
Palaeoecology of the Lower Bengal Basin, Calcutta, India, during the Late Quaternary
Authors: Das Sauren 
Published: October 2009

AbstractPalynological analysis was conducted in view of past ecological reconstruction trends of Calcutta, the Lower Bengal Basin (West Bengal), India. Floristic compositions were derived from peat samples collected from Garia, located at the southern extremity of the city. Garia, being one of the oldest settlements in this region, is situated by the ‘Tolly Nullah’ connecting Calcutta to the Vidhyadhari River at the east. Bore hole samples were radiocarbon dated and three successive peat layers of 7000 (+170) y. B.P. to 2650 (+150) y. B.P. were analyzed for their pollen composition. Pollen data indicates the existance of typical swampy vegetation of mangrove plant community in and around Calcutta, which are presently confined to the Sundarbans forest, the further south of the studied area. The lower most peat layer (ca. 7000–6500 y. B.P.) reveals that the vegetational sequence was comparatively uniform and mainly comprised of Heritiera, Rhizophora, Sonneratia, Excoecaria, Phoenix paludosa and grasses, implying insignificant fluctuations in climatic conditions. The middle layer indicates a mixed vegetational type of fern spores, mangroves and some terrestrial plants (e.g. Acanthus, Cynometra, Suaeda, and grasses). The lower part of the middle peat layer (ca. 6450–6100 y. B.P.) consisted of marshy habitat as evidenced by fern spores, whereas their absence in the upper part implies relatively dry environmental conditions. The upper peat layer (ca. 2600 y. B.P.) is dominated by grass, pollen grains terrestrial habitats (Typha, Cassia, Eucalyptus, Desmodium, Salicornia, and Rhamnaceae) and some of typical mangroves (Heritiera and Excoecaria). Low amounts of fern spores indicating Lower Bengal change towards the terrestrial habitats. The pollen/spore assemblages in the three successive peat layers indicate the presence of typical mangrove vegetation, of the type currently observed in the Sundarbans region. Frequent sea water inundation mixed with fresh water flow from the northern upstream at the low lying topography of Basin seems to have led to the formation of peat by deposition rather than by drifting. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Biological and mycobiota characters of some medicinal plants from Yemen
Authors: Naggar S. M. El , Sallam A. M. H. 
Published: October 2009

AbstractMorphological, palynological and mycobiota characters of five wild medicinal plants from Yemen: Senna italica Mill. (Caesalpiniaceae), Withania somnifera (L.) Dunal (Solanaceae), Commicarpus grandiflorus (A.Rich.) Standl. (Nyctaginaceae), Acalypha fruticosa Forssk. (Euphorbiaceae) and Pulicaria undulata (L.) C.A.Mey. (Asteraceae) have been investigated. Twenty one different fungal species were collected and identified from the leaf surfaces and anther/pollen of the studied medicinal plants. These fungal species represent eight genera on the leaf surfaces and ten genera on the anther/ pollen, respectively. The fungal spectra on leaf surfaces of Senna italica and Acalypha fruticosa were the widest spectra, while this spectrum on the leaves of Withania somnifera was the narrowest one. On the other hand the rugulate pollen of Acalypha fruticosa had the widest spectrum of fungi, while the rugulate or micro‐reticulate pollen of Senna italica had the narrowest one. Members of the fungal genera: Aspergillus, Penicillium and Fusarium were the most frequent taxa on both substratum of the studied medicinal plant species. Some fungal species were restricted to only one substratum (leaf surface or anther/pollen), some others were common. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
SUZ12 is a candidate target of the non‐canonical WNT pathway in the progression of chronic myeloid leukemia
Authors: Pizzatti Luciana , Binato Renata , Cofre Jaime , Gomes Bernadete E. , Dobbin Jane , Haussmann Maria Emilia , D'Azambuja Denise , Bouzas Luis Fernando , Abdelhay Eliana 
Published: October 2009

AbstractPolycomb proteins form multiprotein complexes that repress target genes by chromatin remodeling. In this work, we report that the SUZ12 polycomb gene is over‐expressed in bone marrow samples of patients at the blastic phase of chronic myeloid leukemia. We also found a direct interaction between polycomb group genes and the WNT signaling pathway in chronic myeloid leukemia transformation. Electrophoretic mobility shift assay (EMSA), Chromatin immunoprecipitation assay (ChIP), and mass spectrometry assays identified noncanonical WNT pathway members, such as WNT5A and WNT11, bound to the SUZ12 promoter. Immunohistochemistry and immunofluorescence with WNT5A and WNT11 antibodies confirmed nuclear localization. Knockdown of WNTs 1, 5A, and 11 with RNAi approaches showed that WNT members are capable of activating SUZ12 transcription with varying promoter affinities. Finally, we suggest that SUZ12 is blocking cellular differentiation, as SUZ12 knockdown release differentiation programs in chronic myeloid blastic phase (CML‐BP) transformed cell line. © 2009 Wiley‐Liss, Inc.
Four New Lignans with a Bicyclo[3.3.1]nonadienemethanol Skeleton from Cunninghamia lanceolata
Authors: Lee Ching‐Kuo , Yeh Ming‐Hsi , Lee Tzong‐Huei , Chiu His‐Lin , Kuo Yueh‐Hsiung 
Published: October 2009

AbstractFour unique bicyclo[3.3.1]nonadienemethanol lignans, designated lanceolatanins A (1), B (2), C (3), and D (4), along with one previously known compound, isolariciresinol (5), were isolated from the MeOH extracts of the heartwood of Cunninghamia lanceolata. Their structures were elucidated by application of various spectroscopic methods, including 1D‐ and 2D‐NMR techniques, to their acetylated derivatives 1a, 2a, 3a, and 4a. Their possible biosynthetic formations are also discussed.
Alkaloids from the Roots of Stemona tuberosa
Authors: Hu Jun‐Ping , Yang Dong‐Hui , Lin Wen‐Han , Cai Shao‐Qing 
Published: October 2009

AbstractFour new alkaloids, didehydrotuberostemonine A (1), stemoninone (2), tuberostemospiroline (3), and tuberostemonine L (4), together with seven known alkaloids, were isolated from the roots of Stemona tuberosa. Their structures were elucidated on the basis of spectroscopic analysis. The known alkaloids were identified as 2‐oxostenine (5), tuberostemonine (6), sessilifoliamide H (7), tuberostemonone (8), didehydrotuberostemonine (9), bisdehydrostemoninine (10), and tuberostemoamide (11).
In vivo optical monitoring of tissue pathologies and diseases with vibrational contrast
Authors: Bégin Steve , Bélanger Erik , Laffray Sophie , Vallée Réal , Côté Daniel 
Published: October 2009

AbstractStudies of tissue remodeling require in vivo imaging techniques that are as minimally invasive as possible to avoid microenvironment perturbations. To this end, spontaneous Raman techniques have been used but low signals have limited their application mostly to point spectroscopy measurements. Novel Raman‐based techniques such as coherent and stimulated Raman scattering can overcome this limitation. This manuscript discusses imaging and spectroscopy applications with Raman‐based contrast for in vivo tissue monitoring, and how these can be combined into spectral imaging. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
The role of Runx1/AML1 and Evi‐1 in the regulation of hematopoietic stem cells
Authors: Kumano Keiki , Kurokawa Mineo 
Published: October 2009

AbstractLineage‐specific transcription factors must be precisely regulated during stem cell self‐renewal and lineage commitment decisions. The role of specific transcription factors in hematopoietic stem cell (HSC) fate decisions has derived largely from genetic strategies, primarily gene‐targeting and transgenic or retroviral overexpression experiments. From the previous experimental results, several transcription factors have been found to play critical roles in HSC physiology. Among them, we focus two transcription factors, Runx1/AML1 and Evi‐1, in this review. During embryogenesis, both Runx1 and Evi‐1 are essential for HSCs whereas in the adult, Runx1 and Evi‐1 regulate HSCs negatively and positively, respectively. J. Cell. Physiol. 222: 282–285, 2010. © 2009 Wiley‐Liss, Inc.
Echocardiographic identification of ventricular septal rupture caused by acute stent thrombosis
Authors: Garg Scot , Bourantas Christos V. , Thackray Simon , Alamgir Mohamed F. 
Published: October 2009

AbstractCoronary stenting is an increasingly common procedure. Complications are rare. However, when they do occur, they often require urgent invasive treatment. Investigations that are critical for establishing a diagnosis as well as such guide treatment as a detailed assessment of myocardial morphology and function using transthoracic echocardiography may be overlooked in the haste to treat the patient. We present a case report of subacute drug‐eluting stent thrombosis in which a meticulous echocardiographic examination allowed the identification of a ventricular septal rupture, which ultimately modified treatment. © 2009 Wiley Periodicals, Inc. J Clin Ultrasound, 2010
Change in early‐summer meridional teleconnection over the western North Pacific and East Asia around the late 1970s
Authors: Lin Zhongda , Lu Riyu , Zhou Wen 
Published: October 2009

AbstractPrevious studies have shown that during summer there is a meridional teleconnection over the western North Pacific and East Asia (WNP‐EA) on the interannual time scales. This meridional teleconnection is characterized by the zonally elongated circulation anomalies with alternate signs in the meridional direction over this region. The present study indicates that there is a significant change in the early‐summer meridional teleconnection around the late 1970s, by using ERA‐40 reanalysis data during 1958–2001. Although this meridional teleconnection appears as a dominant mode during the whole analysis period, a close inspection revealed that the teleconnection becomes obscure considerably after the late 1970s. Before the late 1970s, the meridional displacement of the East Asian upper‐tropospheric jet stream (EAJS), which is the most dominant mode of EAJS interannual variability, has a statistically significant relationship with both the zonal shift of the WNP subtropical high and rainfall anomaly in the tropical WNP. After the late 1970s, however, this tropical–extratropical interaction over the WNP‐EA is disrupted. We hypothesize that this change in meridional teleconnection is due to the weakening of vertical easterly shear over the tropical WNP in June after the late 1970s. Before the late 1970s, the easterly vertical shear permits the coupling of external mode and internal mode excited by the tropical WNP precipitation anomaly and results in a significant barotropic response, which is necessary for the meridional teleconnection over the WNP‐EA. After the late 1970s, the near‐zero vertical shear is unfavourable for the coupling and thus weakens the meridional teleconnection. Copyright © 2009 Royal Meteorological Society
Application of pressurized liquid extraction in the analysis of aflatoxins B1, B2, G1 and G2 in nuts
Authors: Campone Luca , Piccinelli Anna Lisa , Aliberti Luigi , Rastrelli Luca 
Published: October 2009

AbstractAflatoxins (AFs) B1, B2, G1 and G2 were extracted from nuts by using pressurized liquid extraction (PLE) and the PLE extracts were analyzed using HPLC with fluorescence detection using photochemical post‐column derivatization without further cleanup procedures. Several extraction parameters such as temperature (25, 40, 60 and 80°C), pressure (500, 1000, 1500 and 2000 psi), solvent extraction mixture (acetone, acetonitrile, ethyl acetate and methanol), number of cycles (1 and 2), use of dispersing agents and cell size (5 and 11 mL) were investigated for their effects on the extraction performance. The results showed 60°C, 1500 psi, acetonitrile, one cycle and a cell size of 5 mL as most favorable PLE operating conditions. The proposed analytical method provides LODs below the maximum levels established by European Union regulations and the recoveries of the four AFs were between 77 and 93% at spiking levels of 4, 2 and 0.5 μg/kg for AFB1 and AFG1 and 1, 0.5 and 0.13 μg/kg for AFB2 and AFG2. Validation was carried out using certified reference materials. PLE has been applied for the first time to the analysis of AFs in nuts and offers the possibility for fast simple and accurate quantitative determination of studied mycotoxins.