bluuebunny/crossref_metadata_2025_split huggingface dataset:
Downregulation of E-Cadherin enhances proliferation of head and neck cancer through transcriptional regulation of EGFR
Authors: Wang Dongsheng , Su Ling , Huang Donghai , Zhang Hongzheng , Shin Dong M , Chen Zhuo G 
Published: September 2011

Abstract

Background
Epidermal growth factor receptor (EGFR) has been reported to downregulate E-cadherin (E-cad); however, whether the downregulation of E-cad has any effect on EGFR expression has not been elucidated. Our previous studies have found an inverse correlation between EGFR and E-cad expression in tissue specimens of squamous cell carcinoma of the head and neck (SCCHN). To understand the biological mechanisms underlying this clinical observation, we knocked down E-cad expression utilizing E-cad siRNA in four SCCHN cell lines.

Results
It was observed that downregulation of E-cad upregulated EGFR expression compared with control siRNA-transfected cells after 72 hours. Cellular membrane localization of EGFR was also increased. Consequently, downstream signaling molecules of the EGFR signaling pathway, p-AKT, and p-ERK, were increased at 72 hours after the transfection with E-cad siRNA. Reverse transcriptase-polymerase chain reaction (RT-PCR) showed EGFR mRNA was upregulated by E-cad siRNA as early as 24 hours. In addition, RT-PCR revealed this upregulation was due to the increase of EGFR mRNA stability, but not protein stability. Sulforhodamine B (SRB) assay indicated growth of E-cad knocked down cells was enhanced up to 2-fold more than that of control siRNA-transfected cells at 72-hours post-transfection. The effect of E-cad reduction on cell proliferation was blocked by treating the E-cad siRNA-transfected cells with 1 μM of the EGFR-specific tyrosine kinase inhibitor erlotinib.

Conclusion
Our results suggest for the first time that reduction of E-cad results in upregulation of EGFR transcriptionally. It also suggests that loss of E-cad may induce proliferation of SCCHN by activating EGFR and its downstream signaling pathways.
MicroRNA signature of cis-platin resistant vs. cis-platin sensitive ovarian cancer cell lines
Authors: Kumar Smriti , Kumar Arooshi , Shah Parag P , Rai Shesh N , Panguluri Siva K , Kakar Sham S 
Published: September 2011

Abstract

Background
Ovarian cancer is the leading cause of death from gynecologic cancer in women worldwide. According to the National Cancer Institute, ovarian cancer has the highest mortality rate among all the reproductive cancers in women. Advanced stage diagnosis and chemo/radio-resistance is a major obstacle in treating advanced ovarian cancer. The most commonly employed chemotherapeutic drug for ovarian cancer treatment is cis-platin. As with most chemotherapeutic drugs, many patients eventually become resistant to cis-platin and therefore, diminishing its effect. The efficacy of current treatments may be improved by increasing the sensitivity of cancer cells to chemo/radiation therapies.

Methods
The present study is focused on identifying the differential expression of regulatory microRNAs (miRNAs) between cis-platin sensitive (A2780), and cis-platin resistant (A2780/CP70) cell lines. Cell proliferation assays were conducted to test the sensitivity of the two cell lines to cis-platin. Differential expression patterns of miRNA between cis-platin sensitive and cis-platin resistant cell lines were analyzed using novel LNA technology.

Results
Our results revealed changes in expression of 11 miRNAs out of 1,500 miRNAs analyzed. Out of the 11 miRNAs identified, 5 were up-regulated in the A2780/CP70 cell line and 6 were down regulated as compared to cis-platin sensitive A2780 cells. Our microRNA data was further validated by quantitative real-time PCR for these selected miRNAs. Ingenuity Pathway Analysis (IPA) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was performed for the selected miRNAs and their putative targets to identify the potential pathways and networks involved in cis-platin resistance.

Conclusions
Our data clearly showed the differential expression of 11 miRNAs in cis-platin resistant cells, which could potentially target many important pathways including MAPK, TGF-β signaling, actin cytoskeleton, ubiquitin mediated proteasomal pathway, Wnt signaling, mTOR signaling, Notch signaling, apoptosis, and many other signaling pathways. Manipulation of one or more of these miRNAs could be an important approach for ovarian cancer chemotherapy.
Managing Delegated Search Over Design Spaces
Authors: Erat Sanjiv , Krishnan Vish 
Published: September 2011

Organizations increasingly seek solutions to their open-ended design problems by employing a contest approach in which search over a solution space is delegated to outside agents. We study this new class of problems, which are costly to specify, pose credibility issues for the focal firm, and require finely tuned awards for meeting the firm's needs. Through an analytical model, we examine the relationship between problem specification, award structure, and breadth of solution space searched by outside agents toward characterizing how a firm should effectively manage such open-ended design contests. Our results independently establish and offer a causal explanation for an interesting phenomenon observed in design contests—clustering of searchers in specific regions of the solution space. The analysis also yields a cautionary finding—although the breadth of search increases with number of searchers, the relationship is strongly sublinear (logarithmic). Finally, from the practical perspective of managing the delegated search process, our results offer rules of thumb on how many and what size awards should be offered, as well as the extent to which firms should undertake problem specification, contingent on the nature (open-endedness and uncertainty) of the design problem solution being delegated to outside agents. This paper was accepted by Kamalini Ramdas, entrepreneurship and innovation.
Parameter estimation for the heat equation on perforated domains
Authors: Banks H. T. , Cioranescu D. , Criner A. K. , Winfree W. P. 
Published: September 2011

Abstract
In this effort we investigate the behavior of a model derived from homogenization theory as the model solution in parameter estimation procedures for simulated data for heat flow in a porous medium. We consider data simulated from a model on a perforated domain with isotropic flow and data simulated from a model on a homogeneous domain with anisotropic flow. We report on both ordinary and generalized least squares parameter estimation procedures.
Moisture Sensitive Adhesives and Flooring Adhesive Failures
Authors: Nelson Peter E. , Hopps Emily R. 
Published: September 2011

Abstract
Over the last decade, changes in environmental government regulations have necessitated the reformulation of many historically durable adhesives used in the application of flooring materials. The most popular flooring adhesives traditionally used solvents containing volatile organic compounds (VOCs), but government-prescribed regulations that limit or restrict VOCs resulted in dramatic changes to most traditional flooring adhesives. Adhesives with VOC-containing solvents were replaced with water-based or 100 % solids adhesive formulations. While these new formulations may reduce the environmental impact of new flooring installations, new adhesives are less durable and more susceptible to moisture-related deterioration. Within the past ten years more moisture-related flooring failures have occurred as adhesive systems have switched away from the proven VOC-containing technology to a newer, low VOC-compliant technology. If the concrete is not properly sealed or allowed to dry, the moisture inherent to concrete substrates can break down, re-emulsify, and dissolve moisture-sensitive flooring adhesives. Consequentially, applied flooring materials can delaminate, buckle, blister, and crack. This paper will compare the composition of newer moisture-sensitive flooring adhesives with that of their VOC-containing predecessors, and describe the properties of the adhesives that reduce overall durability. Case studies of flooring failures resulting from moisture-related deterioration of adhesives will be presented for various flooring materials including carpet tile, sheet vinyl, and vinyl composition tile flooring. Recommendations for repairing failed flooring and providing durable new flooring installations using moisture-sensitive adhesives will also be included.
Atlantic DIP: high prevalence of abnormal glucose tolerance post partum is reduced by breast-feeding in women with prior gestational diabetes mellitus
Authors: O'Reilly Michael W , Avalos Gloria , Dennedy Michael C , O'Sullivan Eoin P , Dunne Fidelma 
Published: September 2011

ObjectiveGestational diabetes (GDM) is associated with adverse fetal and maternal outcomes, and identifies women at risk of future type 2 diabetes mellitus (T2DM). Breast-feeding may improve post partum maternal glucose tolerance. Our objective was to identify the prevalence of post partum dysglycemia after GDM, to delineate associated factors and to examine the effect of lactation on post partum glucose tolerance.DesignWe compared post partum 75 g oral glucose tolerance test (OGTT) results from 300 women with GDM and 220 controls with normal gestational glucose tolerance (NGT) in five regional centers. Breast-feeding data was collected at time of OGTT.MethodsPost partum OGTT results were classified as normal (fasting plasma glucose (FPG) <5.6 mmol/l, 2 h <7.8 mmol/l) and abnormal (impaired fasting glucose (IFG), FPG 5.6–6.9 mmol/l; impaired glucose tolerance (IGT), 2 h glucose 7.8–11 mmol/l; IFG+IGT; T2DM, FPG ≥7 mmol/l±2 h glucose ≥11.1 mmol/l). Binary logistic regression was used to identify factors predictive of persistent hyperglycemia.ResultsFive hundred and twenty women were tested; six (2.7%) with NGT in pregnancy had post partum dysglycemia compared with 57 (19%) with GDM in index pregnancy (P<0.001). Non-European ethnicity (odds ratio (OR) 3.40; 95% confidence interval (CI) 1.45–8.02, P=0.005), family history of T2DM (OR 2.14; 95% CI 1.06–4.32, P=0.034), and gestational insulin use (OR 2.62; 95% CI 1.17–5.87, P=0.019) were associated with persistent dysglycemia. The prevalence of persistent hyperglycemia was significantly lower in women who breast-fed vs bottle-fed post partum (8.2 vs 18.4%, P<0.001).ConclusionsNon-European ethnicity, gestational insulin use, family history of T2DM, and elevated body mass index were associated with persistent dysglycemia after GDM. Breast-feeding may confer beneficial metabolic effects after GDM and should be encouraged.
Electronic Behavior of Ba0.5Sr0.5Co0.8Fe0.2O3-d and SrCo0.9Nb0.1O3-d
Authors: Usiskin Robert E. , Wang Richard Y. , Haile Sossina M. 
Published: September 2011

ABSTRACTThe perovskite Ba0.5Sr0.5Co0.8Fe0.2O3-d (BSCF 5582) has attracted great interest as an oxygen reduction catalyst for solid oxide fuel cells and as an oxygen permeation membrane material. Mixed ionic and electronic conductivity is essential to the high catalytic activity it exhibits, however its electronic behavior and overall defect chemistry are not well understood. The related material SrCo0.9Nb0.1O3-d (SCN 091) is another promising composition that may have comparable performance, but with defect chemistry that is simpler to study. From a combination of thermogravimetric, impedance, and diffraction measurements we find SCN 091 to exhibit somewhat smaller oxygen nonstoichiometry, five times higher electronic conductivity, lower enthalpy of hole migration, and greater structural stability than BSCF 5582. We also observe that the enthalpy of hole migration in such materials tends to increase as oxygen content decreases; the origins of this behavior are unclear.
Spin-On Glass as low temperature gate insulator
Authors: Domínguez Miguel A. , Rosales Pedro , Torres Alfonso , Molina Joel , Moreno Mario , De la Hidalga Francisco J. , Zuñiga Carlos , Calleja Wilfrido 
Published: September 2011

ABSTRACTIn this work, we report the characterization of Spin-On Glass (SOG) as low temperature gate insulator. Our SOG film was deposited at temperature of 200°C, which is compatible to use on flexible substrates. The optical and electrical characterization showed that the refractive index and dielectric constant are very close to those of thermally grown SiO2. Also, analysis of surface roughness by AFM is presented. We demonstrated the use of SOG as gate insulator, fabricating and characterizing inverted staggered a-SiGe:H TFTs. The observed results are promising and suggest that SOG films deposited at 200°C in the Laboratory of Microelectronics of INAOE could be an alternative to improve electrical characteristics of TFTs on low temperature flexible substrates.
Characterization of the surface topography and nano-hardness of Cu/Ni multilayer structures
Authors: Kulej Edyta , Kucharska Barbara , Pyka Grzegorz , Gwoździk Monika 
Published: September 2011

Abstract
This article describes the results of a study of Cu/Ni multilayer coatings applied on a monocrystalline Si(100) silicon substrate by the deposition magnetron sputtering technique. Composed of 100 bilayers each, the multilayers were differentiated by the Ni sublayer thickness (1.2 to 3 nm), while maintaining the constant Cu sublayer thickness (2 nm). The multilayer coatings were characterized by assessing their surface topography using atomic force microscopy and their mechanical properties with nano-hardness measurements by the Berkovich method. The tests showed that the hardness of multilayers was substantially influenced by the thickness ratio of Cu and Ni sublayers and by surface roughness. The highest hardness and, at the same time, the lowest roughness was exhibited by a multilayer structure with a Cu-to-Ni sublayer thickness ratio of 2:1.5.
Dielectric spectroscopy of liquid crystals. Electrodes resistivity and connecting wires inductance influence on dielectric measurements
Authors: Perkowski P. 
Published: September 2011

AbstractDielectric spectroscopy is a very useful experimental method for liquid crystal investigation. Electrodes made from indium tin oxide (ITO) or gold are widely used in measuring cells. During the dielectric spectroscopy measurements performed for smectic liquid crystalline mixture it was found that detection of some important relaxation modes in paraelectric SmA*, ferroelectric SmC* and antiferroelectric SmCA* phases for frequencies higher than 0.2–0.5 MHz is not possible. The measuring setup does not allow us to measure such relaxations due to its own dielectric response covering the dielectric response of liquid crystalline medium. One can observe the spurious contribution for high frequency part of the dielectric spectrum, due to non-zero resistivity of electrode material or non-zero inductivity of connecting wires. In this paper, the new model was introduced. Its final equations show how to calculate parameters of relaxations observed in liquid crystals, from dielectric response of the empty and filled measuring cell. The experimental proof of strong influence of measuring setup properties on effective (measured) values of dielectric permittivities was shown.
Engineering-geological and geotechnical investigation for risk assessment
Authors: May Moufida , Dlala Mahmoud , Bedday Aouicha 
Published: September 2011

AbstractBefore construction activities could begin, engineering geological and geotechnical investigations had to be approved in order to establish a map with suitable areas for safe construction. The example used in this study is Tunis City which has complex geology and geomorphology. The risk analysis was based on a large-scale land-suitability map that was prepared using Geographic Information Systems (GIS). The approach used in this study combined physical data with the geotechnical properties of Tunis City. The adopted methodology and analyses were performed to assess the risk of urban expansion and landscape management. Results are presented as a zoning map that shows the suitable area for safe extension of the urban area. The data used and multi-criterion analysis of geotechnical and geological data seems to be useful for similar case studies and the adopted methodology can be used successfully for identifying similar cities for risk assessment.
Children and New Media: Youth Media Participation. A Case Study of Egypt and Finland
Authors: Hirsjärvi Irma , Tayie Sami 
Published: September 2011

This article focuses on a single case study; the first findings of a qualitative part of the Youth Media Participation (YMP) project in Argentina, Egypt, Finland and India (2009-11) on 10-18 year-old children’s participation through media. Youth Media Participation is funded by the Academy of Finland (2009-11). It collects and analyses three kinds of data from children and young adults; 1) focused interviews collected in Egypt, India and Finland (24 in each country), 2) a questionnaire for statistical data collected from Argentina, Egypt, India and Finland (1,200 in each country, N: 4,800) and 3) media diaries for a separate publication about «One Day of Media» (100 collected in Argentina, Egypt, India, Finland and Kenya, N: 500). The purpose was to undertake an exploratory study to find new ways to approach research questions on children’s participation through media in different countries. The YMP project was launched with focused interviews in Finland and Egypt. This paper focuses on the qualitative, explorative part of the project: the focused interviews that were used to test the original research questions, to explore the many forms of media participation, and to create the questionnaire needed in further research. The project aims at enhancing our understanding of media literacy and its connections to media participation and civic activity.El artículo recoge un estudio de caso único sobre los primeros resultados del análisis cualitativo del proyecto PJM (Youth Media Participation), desarrollado en Argentina, Egipto, Finlandia e India. PJM es apoyado por la Academia de Finlandia (2009-11). Se analizan tres tipos de información provenientes de niños y jóvenes: entrevistas focalizadas en Egipto, India y Finlandia (24 en cada país); cuestionarios para recolección de datos estadísticos de Argentina, Egipto, India y Finlandia (1.200 por país, 4.800 totales); y diarios de medios para una publicación adicional sobre «Un día de medios» (500 totales, 100 obtenidos en Argentina, Egipto, India, Finlandia y Kenia respectivamente). Se indagaba, mediante el estudio exploratorio, nuevos enfoques a preguntas de investigación sobre la participación de niños de distintos países en medios. El proyecto PJM se inició con entrevistas focalizadas en Finlandia y Egipto. Este artículo se centra en la parte cualitativa y exploratoria del proyecto: entrevistas focalizadas para ratificar las preguntas originales de investigación, explorar múltiples formas de participación mediática y crear el cuestionario necesario para investigaciones posteriores. El proyecto busca mejorar nuestra comprensión de la alfabetización mediática y sus vínculos con la participación en los medios y actividad cívica.
The Risk of Emergence of Boomerang Effect in Communication against Violence
Authors: Brändle-Señán Gaspar , Martín-Cárdaba Miguel Ángel , Ruiz-San-Román José Antonio 
Published: September 2011

Violent behaviors cause concern among people, policy makers, politicians, educators, social workers, parents associations, etc. From different fields and perspectives, measures are taken to try to solve the problem of violence. Institutional communication campaigns against violence and the publication of news related to violent events are often some of the actions used by policy makers. But some of the literature and data have shown that its effectiveness is not always exactly as expected. And even some anti-violence messages, can have the opposite effect and reinforce the attitudes of those who thought that violence is necessary. The hypothesis is that most people assume with no problem the core message of anti-violence campaigns. But, and this is the key issue and most problematic, individuals who are more likely to be violent (precisely those who should address such communications) could react to anti-violence message in a violent way. There is a tragic paradox: the anti-violence message could increase the predisposition to violent behavior. This would be a case of what some literature called boomerang effect. This article highlights the need for detailed empirical studies on certain effects of media (desensitization, imitation, accessibility and reactance), which could help explain the emergence of the boomerang effect.Los comportamientos violentos causan inquietud entre los responsables públicos (políticos, educadores, asistentes sociales, asociaciones de madres y padres, etc.) que, desde diversos ámbitos, toman medidas que tratan de dar solución al problema de la violencia. La difusión de campañas institucionales de comunicación en contra de la violencia y el fomento de la publicación de noticias relacionadas con sucesos violentos suelen ser algunas de las acciones utilizadas. No obstante, parte de los datos y de la literatura disponible han demostrado que su eficacia no siempre es la esperada e, incluso, dichas acciones pueden llegar a tener efectos contrarios al deseado y reforzar las actitudes de los que piensan que la violencia es necesaria. Se sostiene la hipótesis de que la mayoría de la población asumiría como propios los mensajes contrarios a la violencia. Sin embargo –y esto es la cuestión clave y más problemática– son justo aquellos individuos con mayor propensión a la violencia (precisamente aquellos a quienes deberían dirigirse tales comunicaciones) quienes podrían reaccionar ante el mensaje antiviolencia de un modo no deseado. Se da una dramática paradoja: el mensaje antiviolencia podría aumentar la predisposición a desarrollar comportamientos violentos. Estaríamos ante un caso de lo que cierta literatura denomina efecto boomerang. Por último, se señala la necesidad de un estudio detallado sobre determinados efectos de los medios de comunicación (insensibilización, imitación, accesibilidad y reactancia), que podrían ayudar a explicar la aparición de dicho efecto boomerang.
Inhibitors of Glioma Growth that Reveal the Tumour to the Immune System
Authors: Nieto-Sampedro Manuel , Valle-Argos Beatriz , Gómez-Nicola Diego , Fernández-Mayoralas Alfonso , Nieto-Díaz Manuel 
Published: September 2011

Treated glioblastoma patients survive from 6 to 14 months. In the first part of this review, we describe glioma origins, cancer stem cells and the genomic alterations that generate dysregulated cell division, with enhanced proliferation and diverse response to radiation and chemotherapy. We review the pathways that mediate tumour cell proliferation, neo-angiogenesis, tumor cell invasion, as well as necrotic and apoptotic cell death. Then, we examine the ability of gliomas to evade and suppress the host immune system, exhibited at the levels of antigen recognition and immune activation, limiting the effective signaling between glioma and host immune cells. The second part of the review presents current therapies and their drawbacks. This is followed by a summary of the work of our laboratory during the past 20 years, on oligosaccharide and glycosphingolipid inhibitors of astroblast and astrocytoma division. Neurostatins, the O-acetylated forms of gangliosides GD1b and GT1b naturally present in mammalian brain, are cytostatic for normal astroblasts, but cytotoxic for rat C6 glioma cells and human astrocytoma grades III and I V, with ID50 values ranging from 200 to 450 nM. The inhibitors do not affect neurons or fibroblasts up to concentrations of 4 μM or higher. At least four different neurostatin-activated, cell-mediated antitumoral processes, lead to tumor destruction: (i) inhibition of tumor neo-vascularization; (ii) activation of microglia; (iii) activation of natural killer (NK) cells; (iv) activation of cytotoxic lymphocytes (CTL). The enhanced antigenicity of neurostatin-treated glioma cells, could be related to their increased expression of connexin 43. Because neurostatins and their analogues show specific activity and no toxicity for normal cells, a clinical trial would be the logical next step.