bluuebunny/crossref_metadata_2025_split huggingface dataset:
The relationship between dental anxiety and dental decay experience in antenatal mothers
Authors: Esa Rashidah , Savithri Vengadasalam , Humphris Gerry , Freeman Ruth 
Published: February 2010

Esa R, Savithri V, Humphris G, Freeman R. The relationship between dental anxiety and dental decay experience in antenatal mothers. Eur J Oral Sci 2010; 118: 59–65. © 2010 The Authors. Journal compilation© 2010 Eur J Oral SciThe aim of this study was to investigate the relationship between dental anxiety and dental decay experience among antenatal mothers attending Maternal and Child Health clinics in Malaysia. A cross‐sectional study was conducted on a consecutive sample of 407 antenatal mothers in Seremban, Malaysia. The questionnaire consisted of participants’ demographic profile and the Dental Fear Survey. The D3cvMFS was employed as the outcome measure and was assessed by a single examiner (intraclass correlation = 0.98). A structural equation model was designed to inspect the relationship between dental anxiety and dental decay experience. The mean Dental Fear Survey score for all participants was 35.1 [95% confidence interval (34.0, 36.3)]. The mean D3cvMFS score was 10.8 [95% confidence interval (9.5, 12.1)]. Participants from low socio‐economic status groups had significantly higher D3cvMFS counts than those from high socio‐economic status groups. The path model with dental anxiety and socio‐economic status as predictors of D3cvMFS showed satisfactory fit. The correlation between dental anxiety and dental decay experience was 0.30 (standardized estimate), indicating a positive association. Socio‐economic status was also statistically significantly associated with the D3cvMFS count (β = 0.19). This study presented robust evidence for the significant relationship between dental anxiety and dental decay experience in antenatal mothers.
Satisfaction with teeth and life‐course predictors: a prospective study of a Swedish 1942 birth cohort
Authors: Ekbäck Gunnar , Nodrehaug‐Åstrøm Anne , Klock Kristin , Ordell Sven , Unell Lennart 
Published: February 2010

Ekbäck G, Nodrehaug‐Åstrøm A, Klock K, Ordell S, Unell L. Satisfaction with teeth and life‐course predictors: a prospective study of a Swedish 1942 birth cohort. Eur J Oral Sci 2010; 118: 66–74. © 2010 The Authors. Journal compilation© 2010 Eur J Oral SciThis study aimed to assess the stability or change in satisfaction with teeth among Swedish adults between the ages of 50 and 65 yr, and to identify the impact of socio‐demographics and of clinical and subjective oral health indicators on participants’ satisfaction with teeth during that period. Self‐administered standardized questionnaires were used as part of a longitudinal study. In 1992, 1997, 2002, and 2007 all residents (born in 1942) of two Swedish counties were invited to participate in the study. A total of 63% women and 66% men reported being satisfied with their teeth between 50 and 65 yr of age. The corresponding figures, with respect to dissatisfaction, were 7% and 6% respectively. Generalized estimated equation models revealed a decline in the odds of being satisfied with advancing age, which was particularly important in subjects with lower education, tooth loss, and smokers. Consolidation in oral health perceptions starts before age 50, suggesting early intervention before that age. Promotion of a healthy adult lifestyle and improved access to quality oral healthcare might increase the likelihood of people being satisfied with their teeth throughout the third age‐period in both genders.
Dentin treatment effects on the bonding performance of self‐adhesive resin cements
Authors: Mazzitelli Claudia , Monticelli Francesca , Toledano Manuel , Ferrari Marco , Osorio Raquel 
Published: February 2010

Mazzitelli C, Monticelli F, Toledano M, Ferrari M, Osorio R. Dentin treatment effects on the bonding performance of self‐adhesive resin cements. Eur J Oral Sci 2010; 118: 80–86. © 2010 The Authors. Journal compilation© 2010 Eur J Oral SciThe effects of dentin surface treatments on the microtensile bond strengths and on the interfacial characteristics of self‐adhesive resin cements were investigated. Flat dentin surfaces were obtained from human molars and were treated as follows: (i) no treatment of dentin; (ii) 0.1 M EDTA scrubbed for 60 s; or (iii) 10% polyacrylic acid applied for 30 s. Three self‐adhesive cements were used to lute composite overlays on deep‐coronal dentin surfaces in the presence of simulated pulpal pressure (15 cm of H2O). Bonded specimens were cut into beams 1 mm thick and stressed in tension until failure. Fractured sticks were examined under a scanning electron microscope. Additional specimens were stained with Masson’s trichrome and observed under light microscopy for interfacial evaluations. The bond strength to dentin of the hydrophobic and solvent‐free cement (RelyX Unicem) was unaffected by the tested dentin treatments. The bond strength of the 2‐hydroxyethyl methacrylate (HEMA)‐based cement (Bis‐Cem) decreased after treatment of dentin with EDTA and/or polyacrylic acid. The hydrophilic and water‐containing cement (G‐Cem) attained a higher bond strength when luted on polyacrylic acid‐conditioned dentin. In conclusion, smear layer removal, opening of dentinal tubules, and the water content of dentin differently influence the bond strengths and the interfacial characteristics of self‐adhesive cement–dentin interfaces.
Down‐regulation of expression of osteoblast and osteocyte markers in periodontal tissues associated with the spontaneous alveolar bone loss of interleukin‐10 knockout mice
Authors: Claudino Marcela , Garlet Thiago P. , Cardoso Cristina R. B. , De Assis Gerson F. , Taga Rumio , Cunha Fernando Q. , Silva João S. , Garlet Gustavo P. 
Published: February 2010

Claudino M, Garlet TP, Cardoso CRB, de Assis GF, Taga R, Cunha FQ, Silva JS, Garlet GP. Down‐regulation of expression of osteoblast and osteocyte markers in periodontal tissues associated with the spontaneous alveolar bone loss of interleukin‐10 knockout mice. Eur J Oral Sci 2010; 118: 19–28. © 2010 The Authors. Journal compilation © 2010 Eur J Oral SciThe aim of this study was to unravel the mechanisms by which interleukin (IL)‐10, a potent pleiotropic cytokine, modulates alveolar bone homeostasis in C57BL/6 wild‐type (WT) and IL‐10 knockout (IL‐10 KO) mice, evaluated at 8, 24, and 48 wk of age. Interleukin‐10 KO mice presented significant alveolar bone loss when compared with WT mice, and this was not associated with changes in leukocyte counts or bacterial load. The levels of expression of messenger RNA (mRNA) for tumor necrosis factor‐α (TNF‐α), IL‐1β, IL‐6, transforming growth factor‐β (TGF‐β), receptor activator of nuclear factor κB ligand (RANKL), osteoprotegerin (OPG), and matrix metalloproteinase 13 (MMP13) were similar between both strains, whereas a significant decrease of tissue inhibitor of metalloproteinase 1 (TIMP1) mRNA expression was found at 48 wk in IL‐10 KO mice. The osteoblast markers core binding factor alpha1 (CBFA1) and type I collagen (COL‐I) were expressed at similar levels in both strains, whereas the levels of alkaline phosphatase (ALP) and osteocalcin (OCN), and those of the osteocyte markers phosphate‐regulating gene endopeptidases (PHEX) and dentin matrix protein 1 (DMP1) were significantly lower in IL‐10 KO mice. Our results demonstrate that the alveolar bone loss in the absence of IL‐10 was associated with a reduced expression of osteoblast and osteocyte markers, an effect independent of microbial, inflammatory or bone‐resorptive pathways.
Aquaporin 11 in the developing mouse submandibular gland
Authors: Larsen Helga S. , Ruus Ann‐Kristin , Schreurs Olav , Galtung Hilde Kanli 
Published: February 2010

Larsen HS, Ruus A‐K, Schreurs O, Kanli Galtung H. Aquaporin 11 in the developing mouse submandibular gland. Eur J Oral Sci 2010; 118: 9–13. © 2010 The Authors. Journal compilation © 2010 Eur J Oral SciSeveral aquaporins (AQPs) have been detected in mature and embryonic mammalian salivary glands (AQP1 and AQP3–AQP8). However, AQP11 has, to our knowledge, never before been described in salivary glands, but is known to be important in, for example, kidney development in mice. We therefore thought it relevant to investigate if AQP11 was present during salivary organogenesis. The submandibular salivary gland (SMG) from CD1 mice was studied during prenatal development and early postnatal development, and also in young adult male and female mice. The expression trend of the AQP11 transcript was detected using the reverse transcription–polymerase chain reaction (RT‐PCR), and the temporal–spatial pattern was observed using in situ hybridization. The AQP11 transcript was first detected at embryonic day 13.5 and showed a more or less constitutive expression trend during the prenatal and early postnatal SMG development. Spatial studies demonstrated that the AQP11 transcript was present in the developing and mature duct structures at all stages studied. In the end pieces, the AQP11 transcript was reduced during glandular development. Our results point to an important role for AQP11 during salivary gland development.
Mechanosensitivity of dental pulp stem cells is related to their osteogenic maturity
Authors: Kraft David C. E. , Bindslev Dorthe A. , Melsen Birte , Abdallah Basem M. , Kassem Moustapha , Klein‐Nulend Jenneke 
Published: February 2010

Kraft DCE, Bindslev DA, Melsen B, Abdallah BM, Kassem M, Klein‐Nulend J. Mechanosensitivity of dental pulp stem cells is related to their osteogenic maturity. Eur J Oral Sci 2010; 118: 29–38. © 2010 The Authors. Journal compilation © 2010 Eur J Oral SciFor engineering bone tissue, mechanosensitive cells are needed for bone (re)modelling. Local bone mass and architecture are affected by mechanical loading, which provokes a cellular response via loading‐induced interstitial fluid flow. We studied whether human dental pulp‐derived mesenchymal stem cells (PDSCs) portraying mature (PDSC‐mature) or immature (PDSC‐immature) bone cell characteristics are responsive to pulsating fluid flow (PFF) in vitro. We also assessed bone formation by PDSCs on hydroxyapatite‐tricalcium phosphate granules after subcutaneous implantation in mice. Cultured PDSC‐mature exhibited higher osteocalcin and alkaline phosphatase gene expression and activity than PDSC‐immature. Pulsating fluid flow (PFF) stimulated nitric oxide production within 5 min by PDSC‐mature but not by PDSC‐immature. In PDSC‐mature, PFF induced prostaglandin E2 production, and cyclooxygenase 2 gene expression was higher than in PDSC‐immature. Implantation of PDSC‐mature resulted in more osteoid deposition and lamellar bone formation than PDSC‐immature. We conclude that PDSCs with a mature osteogenic phenotype are more responsive to pulsating fluid shear stress than osteogenically immature PDSCs and produce more bone in vivo. These data suggest that PDSCs with a mature osteogenic phenotype might be preferable for bone tissue engineering to restore, for example, maxillofacial defects, because they might be able to perform mature bone cell‐specific functions during bone adaptation to mechanical loading in vivo.
The Effect of Platelet‐Rich Plasma on Healing of Palatal Donor Site following Connective Tissue Harvesting: A Pilot Study in Dogs
Authors: Shayesteh Yadollah Soleimani , Eshghyar Nosratollah , Moslemi Neda , Dehghan Mohammad Mehdi , Motahhary Pouria , Ghobadi Zahra , Golestan Banafsheh 
Published: February 2010

ABSTRACTBackground: Peri‐implant plastic surgery includes soft tissue enhancement by connective tissue grafting. The palatal donor site provides peri‐implant keratinized mucosa and soft tissue height. Platelet‐rich plasma (PRP) contains growth factors that may enhance early healing.Purpose: The present animal study investigated the effect of PRP on wound healing of palatal donor site after connective tissue harvesting.Materials and Methods: In 12 mongrel dogs, bilateral palatal connective tissues of 10 × 15 mm were harvested. At test site, PRP was applied into the wound, and the contralateral site served as control. The healing was evaluated clinically and histologically at 1 week, 2 weeks, and 4 weeks after surgeries. Exact binomial probability and Wilcoxon signed‐rank test were used to compare the clinical and histologic measurements.Results: No statistically significant differences between PRP and control sites were measured with regard to clinical healing (p = 1.000) and histologic variables, including inflammatory cells (p = .750), collagen fibers (p = .375), and granulation tissue (p = .500) at any time interval.Conclusion: The addition of PRP to palatal mucosal wound sites did not accelerate wound healing.
Changes in skinfolds and mid‐upper arm circumference during pregnancy in Argentine women
Authors: López Laura Beatriz , Calvo Elvira Beatriz , Poy Mabel Susana , del Valle Balmaceda Yanina , Cámera Karen 
Published: February 2010

AbstractThis investigation describes the pattern of changes in mid‐upper arm circumference (MUAC), triceps, biceps and subscapular skinfold thicknesses during the course of pregnancy, and its relationship with maternal and newborn outcomes. A prospective cohort of 1066 pregnant women were selected in seven different urban regions in Argentina. Measurements of MUAC were carried out at 16, 28 and 36 gestational weeks. In a subsample of 488 women, triceps, biceps and subscapular skinfold thicknesses were measured. Mean total increase in subscapular, tricipital and bicipital skinfolds from 16 to 36 weeks of gestation were 4.5, 3.6 and 2.6 mm, respectively. MUAC showed a mean increase of 1.7 cm in the same period. Overweight or obese women at the start of pregnancy had lower increases in all measurements compared with women with normal or low body mass index. Maternal anthropometry was related to birthweight; women who gave birth to infants of less than 3000 g had lower average values in all measurements than those who had normal birthweight infants. LMS curves for MUAC and skinfolds by gestational age are presented, which can be used as a reference to assess maternal nutrition status during pregnancy. MUAC, tricipital and subscapular skinfold for gestational age curves are proposed for monitoring maternal nutritional status during pregnancy. MUAC cut‐off points of 24.5, 25.5 and 26.5 cm for 16, 28 and 36 weeks of gestation, respectively, are also proposed as a proxy to detect low birthweight.
Understanding barriers to implementing the Norwegian national guidelines for healthy school meals: a case study involving three secondary schools
Authors: Holthe Asle , Larsen Torill , Samdal Oddrun 
Published: February 2010

AbstractThe main goal of the present study was to investigate the barriers to implementing the Norwegian national guidelines for healthy school meals as perceived by principals, project leaders, teachers and students. This study employed a multiple‐case design using an explorative approach. Data were collected at three secondary schools in Norway participating in the intervention project Physical activity and healthy school meals. Individual interviews were conducted with school principals and project leaders, and focus group interviews were conducted separately with teachers and students. Four categories of barriers were identified: (1) lack of adaptation of the guidelines to the target group; (2) lack of resources and funding; (3) conflicting values and goals; and (4) access to unhealthy food outside school. The research identified differences in perceived barriers between the staff responsible for implementation and the students as the target group of the measures. All staff groups perceived barriers relating to both resources, and conflicting values and goals. The teachers were more concerned about issues of relevance for adaptation to the target groups than were the principals and project leaders. The students were concerned mostly with issues directly affecting their experience with items offered in the canteen.
Loose interaction between glyceraldehyde‐3‐phosphate dehydrogenase and phosphoglycerate kinase revealed by fluorescence resonance energy transfer–fluorescence lifetime imaging microscopy in living cells
Authors: Tomokuni Yosuke , Goryo Kenji , Katsura Ayako , Torii Satoru , Yasumoto Ken‐ichi , Kemnitz Klaus , Takada Mamiko , Fukumura Hiroshi , Sogawa Kazuhiro 
Published: February 2010

Loose interaction between the glycolytic enzymes glyceraldehyde‐3‐phosphate dehydrogenase (GAPDH) and phosphoglycerate kinase (PGK) was visualized in living CHO‐K1 cells by fluorescence resonance energy transfer (FRET), using time‐domain fluorescence lifetime imaging microscopy. FRET between active tetrameric subunits of GAPDH linked to cerulean or citrine was observed, and this FRET signal was significantly attenuated by coexpression of PGK. Also, direct interaction between GAPDH–citrine and PGK–cerulean was observed by FRET. The strength of FRET signals between them was dependent on linkers that connect GAPDH to citrine and PGK to cerulean. A coimmunoprecipitation assay using hemagglutinin‐tagged GAPDH and FLAG‐tagged PGK coexpressed in CHO‐K1 cells supported the FRET observation. Taken together, these results demonstrate that a complex of GAPDH and PGK is formed in the cytoplasm of living cells.Structured digital abstract
MINT‐7386555: PGK (uniprotkb:P00558) physically interacts (MI:0915) with GAPDH (uniprotkb:P04406) by anti tag coimmunoprecipitation (MI:0007)
MINT‐7386573: GAPDH (uniprotkb:P04406) and PGK (uniprotkb:P00558) bind (MI:0407) by fluorescent resonance energy transfer (MI:0055)
MINT‐7386590: GAPDH (uniprotkb:P04406) and GAPDH (uniprotkb:P04406) bind (MI:0407) by fluorescent resonance energy transfer (MI:0055)
Mapping the vulnerability of older persons to disasters
Authors: Evans Jeff 
Published: February 2010

Evans J. (2010) Mapping the vulnerability of older persons to disasters. International Journal of Older People Nursing 5, 63–70 doi: 10.1111/j.1748‐3743.2008.00205.xOlder people sit at the centre of a unique constellation of factors that combine to increase their vulnerability to the negative effects of catastrophic events. The aim of this paper is to explore some of the factors that underlie this vulnerability. Attention will be directed at three broad factors that will be discussed in some depth, namely poverty, long standing health conditions and psychological trauma. The aim is not, however, to provide a comprehensive systematic review of existing research evidence regarding the older person and disasters. This is due in part to the paucity of studies that take as their primary focus the older population and disasters. Whilst poverty, chronic illness and psychological issues are discussed in this paper as separate issues, they are interconnected. A map of the synergies between factors is presented that places the older person at the centre of a network of vulnerabilities. Recommendations for strategic planning and clinical practice are made that address this complex problem. The hope is that with insightful preparation the older population can be spared then worse consequences of a disaster when it strikes.
Structuring Sustainable Mobility: A Critical Issue for Geography
Authors: Frändberg Lotta , Vilhelmson Bertil 
Published: February 2010

AbstractThis article presents a geographical perspective on the concept of sustainable mobility. Meeting the challenge of sustainable mobility is a complex matter of promoting sustainable transportation technologies, influencing people’s travel behaviour and transforming society’s social and spatial structures. Arguably, a geographical perspective focuses on the latter, structural dimension of this interplay: how societal demands regarding people’s mobility are continuously shaped and reshaped, and how structuration processes can be transformed in more sustainable directions. This includes the transformation of: (i) physical structures, such as the spatial organization (e.g. the location of activities, land use and transportation systems) of cities and regions, (ii) social contacts and networks distributed in space and time and (iii) cultures of regularly held beliefs, norms and expectations regarding people’s abilities to travel fast and far for any purpose.
Recent Climate Change Impacts on the Boreal Forest of Alaska
Authors: Calef Monika P. 
Published: February 2010

AbstractThe vast boreal forest biome is experiencing rapid climate warming with multiple impacts on the ecosystem which include direct vegetation changes; alterations to the ground thermal and hydrological regimes; and increases in disturbance by fire, insects, and disease. This cold biome serves as one of the major carbon storage reservoirs on this planet and there is much concern that soil warming will result in the release of massive amounts of carbon into the atmosphere where it can cause a significant feedback to global climate change. Climate predictions and observations point to interior Alaska as one of the regions experiencing the most drastic warming and can thus serve as an important harbinger of ecosystem response to climate change.
Purification of Parvalbumin from Carp: A Protocol that Avoids Heat‐Treatment
Authors: Koppelman Stef J. , Romijn Roland A. , De Jongh Harmen H.J. , Nordlee Julie A. , Piersma Sander , Hessing Martin , Taylor Steve L. 
Published: February 2010

ABSTRACT:  Parvalbumin from carp, a major allergen, was purified to homogeneity using ion exchange chromatography and size exclusion chromatography (estimated purity >95% to 98% based on SDS‐PAGE and native PAGE) with a yield of 318 mg, and a number of basic biochemical characteristics were determined. The identity was confirmed by peptide‐mass fingerprinting, and IgE‐binding was demonstrated. The UV/Vis absorbance spectra were explained using the previously published amino acid sequences. Far UV‐CD spectroscopy was used to confirm the folding character of parvalbumin. We conclude that parvalbumin from carp can be purified on a comparatively large (hundreds of milligrams) scale using a purification protocol that does not include denaturing steps. The purified protein resembles biochemical characteristics as were earlier published for carp parvalbumin, that is, a molecular weight of approximately 12 kDa, amino acid sequence identity and a secondary structure containing α‐helices and β‐structures. The described method provides a yield sufficient to produce and characterize antibodies to construct immunochemical methods to detect parvalbumin in food, as well as for use as a standard calibrator for such assays.Practical Application: Parvalbumin is a major allergen from fish. Here, we have purified a comparatively large quantity from carp that can be used to develop antisera for use in an assay method to detect fish allergens.