bluuebunny/crossref_metadata_2025_split huggingface dataset:
TheDrosophila tyramine-beta-hydroxylasegene encodes multiple isoforms with different functions
Authors: Ruppert Manuela , Hampel Stefanie , Sambrani Nagraj , Cibik Osman , Classen Gerbera , Duenisch Andrea , Fuchs Claire , Kell Thomas , Paluri Sravya , Scholz Henrike 
Published: June 2024

AbstractThe Tyramine-beta−hydroxylase (Tbh) is required for octopamine synthesis. To better understand the function of Tbh in neurotransmitter synthesis, we analyzed the molecular genetic organization of theDrosophila melanogaster Tbhgene and found that theTbhgene encodes multiple transcripts. The transcripts differ in their 5’UTR, which results in proteins that differ in their size and putative phosphorylation sites, suggesting that theTbhfunction is regulated at translational and posttranslational levels. We generated a newTbhmutant –TbhDel3- using FLP/FRT recombination mutagenesis to remove the translational start site still that is present inTbhnM18mutants. TheTbhDel3mutants share ethanol tolerance and larval locomotion defects with theTbhnM18mutants. But, they differ in terms of their cellular stress response. To develop normal levels of ethanol tolerance, Tbh is required in a subset of Tbh expressing neurons in the adult brain, which was identified using a newly generatedTbh-Gal4 driver. Taking advantage of a newly generated Tbh antibody serum, we show that one Tbh isoform is expressed in a group of peptidergic Hugin-positive and noradrenergic neurons uncoupling Tbh function from octopamine synthesis. The existence of different functional Tbh isoforms impacts our understanding of the regulatory mechanisms of neurotransmitter synthesis and the function of the octopaminergic neurotransmitter system in cellular processes and the regulation of behavior.Author SummaryVertebrates and insects have structurally identical signaling molecules in their nervous system, such as the neurotransmitter dopamine. But, there are also neurotransmitters that are thought to only occur in the vertebrate or insect brain. Noradrenaline is one such neurotransmitter that regulates flight and fight responses in vertebrates. In insects such as the fruit flyDrosophila melanogaster, the structurally very similar neurotransmitter octopamine is considered to be an invertebrate-specific neurotransmitter that performs similar functions to noradrenaline. The functional similarities also extend to enzymes required for synthesis. Our analysis shows that the enzyme for octopamine synthesis exists in several variations and that the connection between the enzymes and the synthesized neurotransmitter may not be as simple as presumed. Exploiting molecular, behavioral and neuroanatomical studies, we show that different variants might be used in response to different environmental conditions and/or the synthesis of alternative, structurally similar neurotransmitters, such as noradrenaline. These results challenge our view on the functions of octopamine and noradrenaline in the regulation of behavior.
Inclusion, characteristics and credibility of systematic reviews in doctoral theses: A cross-sectional study of all Medical Faculties in Sweden
Authors: Ringsten M. , Färnqvist K. , Bruschettini M. , Johansson M 
Published: June 2024

AbstractObjectiveSystematic reviews (SRs) are essential to ensure that decisions are informed by an up-to-date and complete understanding of the relevant research evidence. Conducting SRs within a doctoral thesis can reduce redundant, harmful and unethical research, identify knowledge gaps, and help the doctoral student obtain important skills to conduct and use research. The output and learning process of SRs overlaps with the aims of doctoral programs. We aim to explore to what extent SRs are included in doctoral theses from all medical faculties in Sweden, and to describe the type, topic and assess the credibility of the reviews.Study design and settingDuplicate assessors independently searched local and national repositories for doctoral theses published in 2021 within all seven medical faculties in Sweden, and categorized identified reviews based on review type, topic, and credibility using AMSTAR-2.Results5.4% (45/852) of all doctoral theses included a review, and 1.3% (45/3461) of all included studies were reviews. Of these, two thirds (31) were SRs and the rest (14) were broader ‘big picture’ reviews. The most common topics were interventions (42%) and exposure/etiology (32%), with no reviews of diagnostic tests. The majority of the SRs had very low (71%) or low (19%) credibility, and few reached a high (7%) or moderate (3%) credibility. The most common issues were limitations with protocols, limited search strategies, and failure to account for risk of bias in drawn conclusions.ConclusionsFew doctoral students included SRs in their theses, and the few SRs included in doctoral theses generally had a low credibility. Increasing the rate and quality of SRs in doctoral theses can help improve quality and relevance of subsequent primary research, and help students develop important skills. Actions are needed to support doctoral students to conduct high quality SRs.What is new?Few doctoral students included systematic reviews (SRs) in their thesesThe few SRs included in doctoral theses generally had a low credibilityIncreasing the rate of SRs can help improve the relevance of subsequent researchMoreover, to support development of important skills and reach educational goalsActions are needed to support doctoral students to conduct high quality SRs
Implementation outcomes of tuberculosis digital adherence technologies: a scoping review using the RE-AIM framework
Authors: Chilala Chimweta I , Foster Nicola , Bahukudumbi Shruti , Mohamed Mona S. , Zary Miranda , Kafie Cedric , Patel Barbie , Gore Genevieve , Schwartzman Kevin , Subbaraman Ramnath , Fielding Katherine 
Published: June 2024

AbstractIntroductionGlobally, tuberculosis (TB) remains one of the leading infectious causes of death, with 1.3 million deaths. Digital adherence technologies (DATs) have the potential to provide person-centred care and improve outcomes. Using the RE-AIM framework, we conducted a scoping review of DAT implementations for TB treatment.MethodsWe searched eight databases for papers published between January 2000 and April 2023, using keywords for ‘tuberculosis’ and ‘digital adherence technology’. Articles meeting prespecified inclusion criteria and containing data on RE-AIM domains were included (PROSPERO-CRD42022326968). We defined ‘reach’ as comprising cellphone ownership and engagement by people with TB (PWTB) with DATs, ‘adoption’ as engagement by healthcare providers with DAT programs, ‘implementation’ as the fidelity of the DAT program implemented, and ‘maintenance’ as longer-term uptake of DATs.ResultsOf 10,313 records, 105 contributed to the synthesis. DATs included SMS, phone, 99DOTS, video-supported therapy and pillboxes. For ‘reach’, across various settings, cellphone access varied from 50-100% and 2-31% of PWTB were excluded from accessing DATs due to technology challenges. 36-100% of PWTB agreed to use a DAT. The weighted mean of DAT engagement over dose-days was 81% for SMS, 85% for phone, 61% for 99DOTS, 87% for pillbox and 82% for VST. Concerning ‘implementation’, the fidelity of DAT implementations was affected by technological issues such as cellphone coverage, DAT malfunction and provider-facing issues; including failure to initiate intensified patient management following low DAT engagement. Findings related to RE-AIM dimensions of ‘adoption’ and ‘maintenance’ were limited.ConclusionOur findings suggest that the ‘reach’ of DATs may be limited by a cascade of barriers, including limitations in cellphone accessibility and suboptimal sustained DAT engagement by PWTB. Video and pillbox DATs have higher levels of engagement. Implementation challenges included technological and provider-facing issues. Improving implementation outcomes may be important for TB DATs to achieve broader public health impact.
Illuminating morphogen and patterning dynamics with optogenetic control of morphogen production
Authors: Benzinger Dirk , Briscoe James 
Published: June 2024

AbstractCells use dynamic spatial and temporal cues to instruct cell fate decisions during development. Morphogens are key examples, where the concentration and duration of morphogen exposure produce distinct cell fates that drive tissue patterning. Studying the dynamics of these processes has been challenging. Here, we establish an optogenetic system for morphogen production that enables the investigation of developmental patterningin vitro. Using a tunable light-inducible gene expression system, we generate long-range Shh gradients that pattern neural progenitors into spatially distinct progenitor domains mimicking the spatial arrangement of neural progenitors found in vivo during vertebrate neural tube development. With this system, we investigate how biochemical features of Shh and the presence of morphogen-interacting proteins affect the patterning length scale. We measure tissue clearance rates, revealing that Shh has an extracellular half-life of about 1h, and we probe how the level and duration of morphogen exposure govern the acquisition and maintenance of cell fates. The rate of Shh turnover is substantially faster than the downstream gene expression dynamics, indicating that the gradient is continually renewed during patterning. Together the optogenetic approach establishes a simple experimental system for the quantitative interrogation of morphogen patterning. Controlling morphogen dynamics in a reproducible manner provides a framework to dissect the interplay between biochemical cues, the biophysics of gradient formation, and the transcriptional programmes underlying developmental patterning.
Interactions between high-intensity light and unrestricted vision in the drive for hyperopia
Authors: Biswas Sayantan , Fianza Busoy Joanna Marie , Barathi Veluchamy A. , Muralidharan Arumugam R. , Schmetterer Leopold , Kathrani Biten K. , Brennan Noel A. , Najjar Raymond P. 
Published: June 2024

AbstractPURPOSETo evaluate the impact of optical versus illuminance factors and their duration-dependency on lens-induced hyperopia (LIH) in chick eyes.METHODSHyperopia was induced in one eye in chicks (10 groups, n=126) from day 1 (D1) post-hatching until D8 using +10 D lenses with fellow eyes as controls. One group (LIH) served as the control without any interventions. Remaining groups were exposed to 2, 4 or 6 hours of unrestricted vision (UnV), high intensity light (HL), or both (HL +UnV). Ocular axial length (AL), refractive error, and choroidal thickness were measured on days 1, 4, and 8. Inter-ocular difference (IOD = experimental - contralateral eye) ± SEM was used to express outcome measures.RESULTSBy D8, LIH decreased AL (-0.42±0.03 mm) and produced hyperopic refraction (+3.48±0.32 D) and choroidal thickening (+85.81±35.23 µm) in the LIH group (all, P<0.001). Exposure to UnV reduced LIH (i.e., hyperopic refraction, axial shortening, and choroidal thickening) in a duration-dependent manner, whereas HL potentiated the development of LIH in a duration-dependent manner. When combined, UnV overpowered HL, with resultant impact on refraction and AL being close to UnV alone, except after 6 hours, when HL+UnV induced shorter AL compared to UnV alone (P=0.03).CONCLUSIONDaily exposure to HL, UnV, and HL+UnV altered LIH in a duration-dependent manner with UnV and LIH producing competing signals. The signal generated by UnV was generally stronger than HL in combined exposure, yet longer durations of HL affected the drive for emmetropization in eyes with UnV.
Increase in ER-mitochondria contacts and mitochondrial fusion are hallmarks of mitochondrial activation during embryogenesis
Authors: Chugunova Anastasia , Keresztes Hannah , Kobylinska Roksolana , Novatchkova Maria , Lendl Thomas , Strobl Marcus , Schutzbier Michael , Dürnberger Gerhard , Imre Richard , Roitinger Elisabeth , Pasierbek Pawel , Cencerrado Alberto Moreno , Brandstetter Marlene , Köcher Thomas , Agerer Benedikt , Genger Jakob-Wendelin , Bergthaler Andreas , Pauli Andrea 
Published: June 2024

SummaryMitochondrial ATP production is essential for development, yet the mechanisms underlying the continuous increase in mitochondrial activity during embryogenesis remain elusive. Using zebrafish as a model system for vertebrate development, we comprehensively profile mitochondrial activity, morphology, metabolome, proteome and phospho-proteome as well as respiratory chain enzymatic activity. Our data show that the increase in mitochondrial activity during embryogenesis does not require mitochondrial biogenesis, is not limited by metabolic substrates at early stages, and occurs without an increase in the abundance of respiratory chain complexes or theirin vitroactivity. Our analyses pinpoint a previously unexplored increase in mitochondrial-ER association during early stages in combination with changes in mitochondrial morphology at later stages as possible contributors to the rise in mitochondrial activity during embryogenesis. Overall, our systematic profiling of the molecular and morphological changes to mitochondria during embryogenesis provides a valuable resource for further studying mitochondrial function during embryogenesis.
Parallel tempered metadynamics: Overcoming potential barriers without surfing or tunneling
Authors: Eichhorn Timo , Fuwa Gianluca , Hoelbling Christian , Varnhorst Lukas 
Published: June 2024

At fine lattice spacings, Markov chain Monte Carlo simulations of QCD and other gauge theories with or without fermions are plagued by slow modes that give rise to large autocorrelation times. This can lead to simulation runs that are effectively stuck in one topological sector, a problem known as topological freezing. Here, we demonstrate that for a relevant set of parameters, metadynamics can be used to unfreeze four-dimensional SU(3) gauge theory. However, compared to local update algorithms and the Hybrid Monte Carlo algorithm, the computational overhead is significant in pure gauge theory, and the required reweighting procedure may considerably reduce the effective sample size. To deal with the latter problem, we propose modifications to the metadynamics bias potential and the combination of metadynamics with parallel tempering. We test the new algorithm in four-dimensional SU(3) gauge theory and find that it can achieve topological unfreezing without compromising the effective sample size, thereby reducing the autocorrelation times of topological observables by at least 2 orders of magnitude compared to conventional update algorithms. Additionally, we observe significantly improved scaling of autocorrelation times with the lattice spacing in two-dimensional U(1) gauge theory.

Published by the American Physical Society
2024
Determinants of organic food purchase behaviour in the European Union: a cross-country analysis guided by the theory of planned behavior
Authors: Serebrennikov Dmytro , Kallas Zein , Thorne Fiona , Ornelas Herrera Selene Ivette , McCarthy Sinéad N. 
Published: June 2024

PurposeThe purpose of this study is to analyse the impact of behavioural determinants, such as consumer attitude, subjective norm, behavioural control and sustainable buying behaviour, on organic food purchase behaviour in different EU countries.Design/methodology/approachA structured survey of more than 5000 individuals from Germany, Netherlands, Spain, Italy and Czech Republic was conducted to elicit consumer responses regarding behavioural determinants of organic food selection. Both a structural equation modelling approach and a multinomial logit model were employed to analyse the data.FindingsConsumer attitudes, subjective norms and behavioural control were shown to significantly influence the frequency of organic food purchasing behaviour for the whole sample and to varying degrees across each of the countries. The effect of a sustainable buying behaviour was found to be more pronounced for Germany, Netherlands and Italy than for Czech Republic and Spain.Research limitations/implicationsAnalysis is based on self-reported data on frequency of organic food purchases, which might be subject to recall bias. Overestimation may arise as consumers tend to report increased purchasing due to the “desirable” qualities of organic food. We tried to ensure that the translations bore similar meanings cross country although some miscomprehension by survey respondents was a possibility.Practical implicationsTo stimulate consumer demand for organic products, it is important to design policies and interventions that take into account the impact of both behavioural factors and demographic attributes on consumer decision-making across various locations. For example, market analysts and policymakers may find it useful to exploit the impact of consumer attitude towards the qualities of organics to induce sales of such food in their locations.Originality/valueThis paper combines unique survey data from five EU countries using a common model to explore and compare consumer behavioural preferences for multiple organic food products. In addition to a classic triad of behavioural factors believed to influence organic food purchases, sustainable buying behaviour as a determinant of organic purchasing behaviour was included in the analysis.
Perfectionism damages performance: shifting the dial through learning and development interventions
Authors: Kohli Kanika , Prabhu Nandan 
Published: June 2024

Purpose
This article aims to explore the relationship between perfectionism and job performance to suggest opportunities for organizational learning and development.

Design/methodology/approach
The article examines and contextualizes existing research on perfectionism for organizational learning and development. This study identifies gaps and puzzles in the perfectionism-performance relationship and provides practical insights for leaders and managers.

Findings
The study's findings suggest trends in the perfectionism-job performance relationship and have practical implications for organizations. The study also proposes practical ideas, a framework for future research and interventions that organizations can use to promote excellence in the workplace.

Originality/value
This article is original in its focus on the relationship between perfectionism and job performance, and it contributes to both research and practice by discussing the academic and practical aspects of perfectionism in the workplace.
Proactive cyber fraud response: a comprehensive framework from detection to mitigation in banks
Authors: Chhabra Roy Neha , P. Sreeleakha 
Published: June 2024

Purpose
This study addresses the ever-increasing cyber risks confronting the global banking sector, particularly in India, amid rapid technological advancements. The purpose of this study is to de velop an innovative cyber fraud (CF) response system that effectively controls cyber threats, prioritizes fraud, detects early warning signs (EWS) and suggests mitigation measures.

Design/methodology/approach
The methodology involves a detailed literature review on fraud identification, assessment methods, prevention techniques and a theoretical model for fraud prevention. Machine learning-based data analysis, using self-organizing maps, is used to assess the severity of CF dynamically and in real-time.

Findings
Findings reveal the multifaceted nature of CF, emphasizing the need for tailored control measures and a shift from reactive to proactive mitigation. The study introduces a paradigm shift by viewing each CF as a unique “fraud event,” incorporating EWS as a proactive intervention. This innovative approach distinguishes the study, allowing for the efficient prioritization of CFs.

Practical implications
The practical implications of such a study lie in its potential to enhance the banking sector’s resilience to cyber threats, safeguarding stability, reputation and overall risk management.

Originality/value
The originality stems from proposing a comprehensive framework that combines machine learning, EWS and a proactive mitigation model, addressing critical gaps in existing cyber security systems.
Research trends in international public sector accounting standards (IPSAS): a bibliometric analysis
Authors: Tijjani Bashir , Algarni Ahmed Ali , Al Hudithi Faisal Abdullah , Falgi Khalid Idris , Siddique Nadeem , Khan Muhammad Ajmal 
Published: June 2024

Purpose
This study aims to provide quantitative information on the research trends of international public sector accounting standards (IPSAS).

Design/methodology/approach
The study used bibliometric analysis to determine the research trends in IPSAS literature. The method was applied to papers indexed in the Scopus database to analyze the growth and publication trends of the selected papers. The research team rigorously compiled a list of keywords relevant to IPSAS after consultation with the principal researcher. The study did not apply document type, year or language filter. The search query resulted in 475 documents. The study used MS Access, Excel and Biblioshiny software for the data analysis and visualization.

Findings
The acceptance and application of IPSAS by many countries and UN system organizations have led to the rise in IPSAS literature globally. The study finds the USA to be the most productive country, with Italy, Spain and the UK as second, third and fourth, respectively. Similarly, the University of Zaragoza has produced the most publications in the field, followed by the University of Salerno and Ghent University.

Practical implications
This study provides the current state of IPSAS research globally; as a result, it supports the development of policies by the users of this standards. This information can inform the development or revision of public sector accounting policies and standards.

Originality/value
To the best of the authors’ knowledge, this is the first study that provides a bibliometric analysis of the growth and publication trends of IPSAS literature. The findings in this study uncover different areas of IPSAS research productivity globally.
Antecedents and outcomes of customer over-the-top experience: a systematic literature review
Authors: Kalra Neha , Deshwal Pankaj , Gokarn Samir , Kushwah Shiksha 
Published: June 2024

PurposeThe proliferation of technological advancements has facilitated unrestricted access to and customizable consumption of content for viewers. Over-the-top (OTT) services are becoming more and more popular as the number of people using video streaming services grows around the world. In this context, this study aims to identify the antecedents and outcomes of Customer over-the-top Experience (COTTE) by synthesizing the existing research.Design/methodology/approachThis research used the systematic literature review approach to identify the antecedents and outcomes of COTTE, along with the publication schedule, theories, analytical techniques, research methodology, and geographic scope of the 47 studies identified from the Scopus and Web of Science database.FindingsThe findings elucidate various antecedents of COTTE, including user-related, social, content-related, and website/platform-related factors. Additionally, diverse outcomes, encompassing behavioural/attitudinal and company-related factors have been discussed. Furthermore, an integrated framework is presented herein, synthesizing extant research and guiding future researchers in this domain.Originality/valueThe study’s findings offer a novel perspective for service providers aiming to enhance the OTT experience for their customers. This study stands out as one of the first to comprehensively present the antecedents and consequences of COTTE.