bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous Dual Vascular Access Site for the Treatment of Coronary Artery Bifurcation Lesions by Complex 2‐Stent Technique
Authors: ISAAZ KARL , MAYAUD NORBERT , LAMAUD MICHEL , RAYNAUD AMÉLIE , CERISIER ALEXIS , SABRY MOHAMMED HASSAN , RICHARD LAURE , KHAMIS HAZEM , ABD‐ALAZIZ AHMAD , DA COSTA ANTOINE 
Published: June 2012

Objectives:
To propose an original approach based on simultaneous dual vascular access site (DAS) using 2 small‐size guiding catheters to easily perform complex 2‐stent techniques for bifurcation coronary lesions (BL).
Background:
Simultaneous kissing stenting and classic crush technique require large 7 or 8Fr guiding catheters leading to large amounts of contrast medium, vascular access site complications, and sometimes frictions or criss‐cross of the 2‐stent delivery systems.
Methods:
DAS was used in 30 patients with BL (11 radio‐radial, 16 radio‐femoral, and 3 femoro‐femoral). Among 60 guiding catheters, the size was 5Fr in 28, 6Fr in 30, and 7Fr in 2 cases of double adjacent BL. When 2 different size catheters were used, contrast medium injections were done using the smallest size catheter. DAS patients were compared with a group of 30 BL patients treated using a single femoral vascular access site (SAS) with 7 or 8Fr catheters.
Results:
Success rate was 100% in all patients. Contrast volume used was smaller in DAS than in SAS patients (277 ± 156 cc vs. 380 ± 165 cc,P = 0.01). No vascular access site complication occurred in the sub‐group of the 11 DAS radio‐radial patients. Postintervention hospitalization duration was shorter in DAS than in SAS (1.9 ± 2 vs. 2.8 ± 2 days,P = 0.048).
Conclusion:
DAS allows to successfully perform complex stenting technique of BL using small‐size guiding catheters leading to decreased contrast medium volume, decreased vascular access site complications rates, and shortened hospitalization duration. (J Interven Cardiol 2012;25:439–446)
Coronary Angioplasty and the Internet: What Can Patients Searching Online Expect to Find?
Authors: KIRTHI VARO , MODI BHAVIK N. 
Published: June 2012

Objectives:
This study set out to assess the quality of online information available on coronary angioplasty.
Background:
Patients searching for healthcare information frequently use the Internet. However the lay reader may not be able to discern the robustness of evidence presented. At present, the overall quality and accuracy of online content regarding coronary angioplasty is unknown.
Methods:
The search term “coronary angioplasty” was entered into three popular search engines (Google, Yahoo, and Bing), and the first 50 webpages provided by each search engine pooled. Exclusion criteria consisted of duplicated sites, sites requiring a registration or login, and direct links to documents or videos. The remaining sites were analyzed using the LIDA instrument; a validated method for assessing websites based on accessibility, usability, and readability. Readability was also separately assessed using the Flesch Reading Ease Score (FRES).
Results:
Of 150 weblinks reviewed, 86 were excluded on the basis of the criteria listed above. The remaining 64 sites achieved mean scores of 50/60 (83%) on accessibility, 40/54 (74%) on usability, 32/51 (62%) on reliability, and 47.5 on FRES. Significant variability was noted among the LIDA scores, with no correlation between LIDA score and search engine ranking.
Conclusions:
Although most websites are easy to access, content is frequently out‐of‐date and fails to be presented in an easily comprehensible format. A minority of websites display factually incorrect information. Clinicians should be wary of patients being misled by erroneous or commercially biased online content, and be able to redirect their patients to more robust, up‐to‐date sources. (J Interven Cardiol 2012;25:476–481)
Long‐term compliance with follow‐up after treatment for cervical intra‐epithelial neoplasia
Authors: SOUTTER WILLIAM PATRICK , MOSS BRONWEN , PERRYMAN KAREN , KYRGIOU MARIA , PAPAKONSTANTINOU KATERINA , GHAEM‐MAGHAMI SADAF 
Published: June 2012

AbstractObjective. To assess the level of compliance with follow‐up over time after treatment for preinvasive and Stage Ia1 lesions of the cervix. To compare the average interval between visits with the interval prescribed by protocol and to evaluate the use of this difference as a measure of compliance. Design. Retrospective observational study. Setting. London university hospital. Population. Women who received treatment for preinvasive (CINI‐III, AIS) or Stage Ia1 cervical lesions. Methods. Attendance data were obtained from hospital‐based colposcopy and community‐based databases. Main outcome measures. The average interval between the appointments attended by each woman was compared with the planned interval between visits. The time that elapsed since the previous appointment attended was calculated for each visit and correlated with the time since treatment. Results. 1013 women attended 4128 follow‐up visits in the colposcopy clinic and in the community. Twenty‐two (2.2%) women never attended any post‐treatment appointment and 209 (21.0%) of the 991 women who attended at least once, did so on average more than 12 months later than specified by the follow‐up protocol. There was a highly significant correlation between the interval since the previous appointment and the time since treatment (Kendall's tau b = 0.529614, two‐sided p < 0.0001), showing that compliance deteriorates with increasing time since treatment. Conclusions. Comparing the average interval between attendances with the planned interval prescribed by protocol is a sensitive measure of compliance. Compliance with follow‐up deteriorates significantly with time since treatment. This decline in compliance may contribute to the increased risk of invasive disease after treatment.
Factors associated with and causes of perinatal mortality in northeastern Tanzania
Authors: SCHMIEGELOW CHRISTENTZE , MINJA DANIEL , OESTERHOLT MAYKE , PEHRSON CAROLINE , SUHRS HANNAH ELENA , BOSTRÖM STÉPHANIE , LEMNGE MARTHA , MAGISTRADO PAMELA , RASCH VIBEKE , LUSINGU JOHN , THEANDER THOR G. , NIELSEN BIRGITTE BRUUN 
Published: June 2012

AbstractObjective. To identify factors associated with perinatal mortality in northeastern Tanzania. Design. Prospective cohort study. Setting. Northeastern Tanzania. Population. 872 mothers and their newborns. Methods. Pregnant women were screened for factors possibly associated with perinatal mortality, including preeclampsia, small‐for‐gestational age, preterm delivery, anemia, and health‐seeking behavior. Fetal growth was monitored using ultrasound. Finally, the specific causes of the perinatal deaths were evaluated. Main outcome measure. Perinatal mortality. Results. Forty‐six deaths occurred. Key factors associated with perinatal mortality were preterm delivery (adjusted odds ratio (OR) 14.47, 95% confidence interval (CI) 3.23–64.86, p < 0.001), small‐for‐gestational age (adjusted OR 3.54, 95%CI 1.18–10.61, p = 0.02), and maternal anemia (adjusted OR 10.34, 95%CI 1.89–56.52, p = 0.007). Adherence to the antenatal care program (adjusted OR 0.027, 95%CI 0.003–0.26, p = 0.002) protected against perinatal mortality. The cause of death in 43% of cases was attributed to complications related to labor and specifically to intrapartum asphyxia (30%) and neonatal infection (13%). Among the remaining deaths, 27% (7/26) were attributed to preeclampsia and 23% (6/26) to small‐for‐gestational age. Of these, 54% (14/26) were preterm. Conclusions. Preeclampsia, small‐for‐gestational age and preterm delivery were key risk factors and causes of perinatal mortality in this area of Tanzania. Maternal anemia was also strongly associated with perinatal mortality. Furthermore, asphyxia accounted for a large proportion of the perinatal deaths. Interventions should target the prevention and handling of these conditions in order to reduce perinatal mortality.
Melanoma recognition framework based on expert definition of ABCD for dermoscopic images
Authors: Abbas Qaisar , Emre Celebi M. , Garcia Irene Fondón , Ahmad Waqar 
Published: June 2012

Background/purposeMelanoma Recognition based on clinical ABCD rule is widely used for clinical diagnosis of pigmented skin lesions in dermoscopy images. However, the current computer‐aided diagnostic (CAD) systems for classification between malignant and nevus lesions using the ABCD criteria are imperfect due to use of ineffective computerized techniques.MethodsIn this study, a novel melanoma recognition system (MRS) is presented by focusing more on extracting features from the lesions using ABCD criteria. The complete MRS system consists of the following six major steps: transformation to the CIEL*a*b* color space, preprocessing to enhance the tumor region, black‐frame and hair artifacts removal, tumor‐area segmentation, quantification of feature using ABCD criteria and normalization, and finally feature selection and classification.ResultsThe MRS system for melanoma‐nevus lesions is tested on a total of 120 dermoscopic images. To test the performance of the MRS diagnostic classifier, the area under the receiver operating characteristics curve (AUC) is utilized. The proposed classifier achieved a sensitivity of 88.2%, specificity of 91.3%, and AUC of 0.880.ConclusionsThe experimental results show that the proposed MRS system can accurately distinguish between malignant and benign lesions. The MRS technique is fully automatic and can easily integrate to an existing CAD system. To increase the classification accuracy of MRS, the CASH pattern recognition technique, visual inspection of dermatologist, contextual information from the patients, and the histopathological tests can be included to investigate the impact with this system.
Proposal for an in vivo histopathologic scoring system for skin aging by means of confocal microscopy
Authors: Longo Caterina , Casari Alice , De Pace Barbara , Simonazzi Silvia , Mazzaglia Giovanna , Pellacani Giovanni 
Published: June 2012

BackgroundMany instrumental devices have been testing in analysing and quantifying the skin aging signs. However, histopathology still remains the only methods that allow a microscopic assessment of the skin. However, a skin biopsy is not feasible in aesthetically critical areas such as the face. Recently, confocal microscopy has been discovered as a noninvasive tool with a nearly histologic resolution. Distinct morphologic confocal aspects on facial skin have been described and correlated with the histopathologic counterparts.ObjectivesIn our study we aim to develop an easy to use confocal aging score to quantify the skin aging related signs.MethodsA sample of facial skin of fifty volunteers has been subjected to confocal imaging. Combining the previously identified confocal features, three different semi‐quantitative scores were calculated: – epidermal disarray score (irregular honeycombed pattern + epidermal thickness + furrow pattern); – epidermal hyperplasia score (mottled pigmentation + extent of polycyclic papillary + epidermal thickness; – collagen score (curled fibers, 2 for huddles of collagen, 1 for coarse collagen structures, and 0 for thin reticulated collagen)ResultsThe epidermal disarray score showed a stable trend up to 65 years and a dramatic increase in the elderly subjects epidermal. Hyperplasia score was characterized by an ascending trend from younger subjects to middle age. The total collagen score showed a progressive trend with age with a different proportion of distinct collagen type.ConclusionsRCM is a powerful, noninvasive technique that could permit to microscopically quantify the aging signs and to test cosmetic efficacy.
Vitamin K1–loaded lipid‐core nanocapsules: physicochemical characterization and in vitro skin permeation
Authors: da Silva Ana Luiza M. , Contri Renata V. , Jornada Denise S. , Pohlmann Adriana R. , Guterres Silvia S. 
Published: June 2012

BackgroundThe incorporation of substances in nanocarriers can modulate and/or manage their delivery profiles (immediate or sustained) and permeation through skin. Consequently, drug nanencapsulation intended for topical treatment can reduce the systemic absorption of the substance.ObjectiveTo obtain and characterize vitamin K1–loaded lipid core nanocapsules as well as to determine whether the nanoencapsulation influences the skin permeation of this vitamin.MethodsThe skin permeation study was performed by means of Franz‐type diffusion cells followed by the tape stripping and retention techniques. The vitamin K1–loaded lipid core nanocapsules were obtained by the preformed polymer precipitation method and the particles were characterized.ResultsThe nanocapsules presented average diameter of 211 ± 2 nm, pH of 5.7 ± 0.3, zeta potential of –14.9 ± 0.6 mV and drug content of 10.2 mg/mL (102.1%). The physical stability of the nanocapsule suspension was verified using multiple light backscattering analysis. The amount of vitamin K1 in the dermis after 8 h of drug permeation was higher when the nanocapsules were applied compared to the control. Moreover, retention in the outermost skin layer and a decrease in the skin permeation to the receptor compartment due to the nanoencapsulation were observed.ConclusionThus, nanoencapsulation can lead to the selective permeation of vitamin K1 through the skin.
Softness sensor system for simultaneously measuring the mechanical properties of superficial skin layer and whole skin
Authors: Nakatani Masashi , Fukuda Toru , Arakawa Naomi , Kawasoe Tomoyuki , Omata Sadao 
Published: June 2012

Background/AimsFew attempts have been made to distinguish the softness of different skin layers, though specific measurement of the superficial layer would be useful for evaluating the emollient effect of cosmetics and for diagnosis of skin diseases.Materials and methodsWe developed a sensor probe consisting of a piezoelectric tactile sensor and a load cell. To evaluate it, we firstly measured silicone rubber samples with different softness. Then, it was applied to human forearm skin before and after tape‐stripping. A VapoMeter and skin‐surface hygrometer were used to confirm removal of the stratum corneum. A Cutometer was used to obtain conventional softness data for comparison.Results and conclusionsBoth the piezoelectric tactile sensor and the load cell could measure the softness of silicone rubber samples, but the piezoelectric tactile sensor was more sensitive than the load cell when the reaction force of the measured sample was under 100 mN in response to a 2‐mm indentation. For human skin in vivo, transepidermal water loss and skin conductance were significantly changed after tape‐stripping, confirming removal of the stratum corneum. The piezoelectric tactile sensor detected a significant change after tape‐stripping, whereas the load cell did not. Thus, the piezoelectric tactile sensor can detect changes of mechanical properties at the skin surface. The load cell data were in agreement with Cutometer measurements, which showed no change in representative skin elasticity parameters after tape‐stripping. These results indicate that our sensor can simultaneously measure the mechanical properties of the superficial skin layer and whole skin.
Capturing skin properties from dynamic mechanical analyses
Authors: Sandford Erika , Chen Yi , Hunter Ian , Hillebrand Greg , Jones Lynette 
Published: June 2012

Background/purposeExisting skin mechanical testing devices focus on measuring skin elasticity and are not tailored to assess the dynamic behavior of skin. Additionally, the mathematical techniques used in existing devices are often not optimal.MethodsA new dynamic mechanical device that measures the linear dynamics of skin was developed and tested. The mechanical properties of skin were evaluated in experiments in which stiffness and damping parameters were measured (i) at different locations on the arm and hand, (ii) when stratum corneum hydration was varied by controlled changes in environmental humidity, and (iii) following the application of film‐forming polymers. Parallel measurements were made with the Cutometer® so that the two devices could be compared.ResultsThe findings revealed that reliable and valid measurements of skin mechanical properties can be obtained from the device. The stiffness of the skin was shown to vary significantly as a function of skin site, changes in stratum corneum hydration and following application of the polymer films. Changes in the damping parameter were less consistently associated with varying the condition of the skin.ConclusionThe high reliability and speed of measurement make this device and analytic procedure an attractive option for testing skin mechanics.
Commensal bacteria coated by secretory immunoglobulin A and immunoglobulin G in the gastrointestinal tract of pigs and calves
Authors: TSURUTA Takeshi , INOUE Ryo , TSUKAHARA Takamitsu , NAKAMOTO Mitsunori , HARA Hiroshi , USHIDA Kazunari , YAJIMA Takaji 
Published: June 2012

ABSTRACTA large amount of secretory immunoglobulin A (S‐IgA) is secreted in the alimentary tract of mammals. It has been reported that S‐IgA coats a portion of commensal intestinal bacteria in human and mouse. However, S‐IgA‐coated bacteria have not been studied in pigs and calves. In this study, we evaluated the distribution of S‐IgA‐coated commensal intestinal bacteria in each portion of the gastrointestinal tracts of pigs and calves. Immunoglobulin G (IgG)‐coated bacteria were also analyzed because a considerable amount of IgG is secreted in the gastrointestinal tracts of pigs, and in particular, calves. S‐IgA‐ or IgG‐coated bacteria were detected in all the segments of the gastrointestinal tracts of pigs and calves. The proportion of S‐IgA‐coated bacteria to total bacteria (i.e. S‐IgA coating ratio) varied in the segments of the gastrointestinal tract in pigs, whereas those of calves were nearly the same throughout the gastrointestinal tract. The S‐IgA and IgG coating ratios were higher in pigs than in calves for all segments of the gastrointestinal tract.
Effects of complete dentures on respiratory performance: spirometric evaluation
Authors: Piskin Bulent , Sipahi Cumhur , Karakoc Omer , Atay Arzu , Ciftci Faruk , Tasci Canturk , Akin Hakan , Arisan Volkan , Sevketbeyoglu Haldun , Turker Turker 
Published: June 2012

ObjectivesThere is a lack of data regarding whether edentulous subjects should remove dentures during spirometric measurements or not. The purpose of this study is to determine influences of complete dentures on spirometric parameters in edentulous subjects.Materials and methodsA total of 46 complete denture wearers were included in this study. Respiratory functions of the subjects were evaluated by spirometric tests that were performed in four different oral conditions: without dentures (WOD), with dentures, lower denture only and upper denture only. Forced vital capacity (FVC), peak expiratory flow, forced expiratory volume in 1 s and forced expiratory flow between 25% and 75% were evaluated. The data were analyzed with Friedman, Wilcoxon and paired‐samples t tests (α = 0.05).ResultsSignificant differences were found between spirometric parameters in different oral conditions (p < 0.05). In all spirometric parameters, the most important significant differences were found between conditions WOD, FVC and with lower dentures (FVC), and WOD (forced expiratory volume in 1 s) and with upper dentures (forced expiratory volume in 1 s) (p < 0.001).ConclusionIt was observed that complete dentures may unfavourably affect spirometric values of edentulous subjects. However, current findings need to be confirmed with advanced respiratory function tests.
Detection of the rare HLA‐B*40:97 allele in an unrelated Taiwanese bone marrow donor
Authors: Yang K. L. , Lee S. K. , Kao R. H. , Lin C. L. , Lin P. Y. 
Published: June 2012

SummaryWe detected a rare HLA‐B locus allele, B*40:97, in a Taiwanese unrelated donor in our routine HLA SBT (sequence‐based typing) exercise for a possible hematopoietic stem cell donation. In exons 2, 3 and 4, the sequence of B*40:97 is identical to the sequence of B*40:02:01 except one nucleotide at nucleotide position 760 (C‐>T) in exon 4. The nucleotide variation caused one amino acid alteration at residue 230 (L‐>F). B*40:97 was probably derived from a nucleotide substitution event where C was replaced by T at nucleotide 760 involving B*40:02:01. The HLA‐A, HLA‐B, HLA‐C, HLA‐DRB1 and HLA‐DQB1 haplotype in association with B*40:97 may be deduced as A*26:01‐B*40:97‐C*03:03‐DRB1*11:01‐DQB1*03:03. Our recognition of B*40:97 in Taiwanese helps to fill the void of ethnic information for the allele B*40:97 reported to the IMGT/HLA Database.
Co‐expression Analysis Identifies CRC and AP1 the Regulator of Arabidopsis Fatty Acid Biosynthesis
Authors: Han Xinxin , Yin Linlin , Xue Hongwei 
Published: June 2012

AbstractFatty acids (FAs) play crucial rules in signal transduction and plant development, however, the regulation of FA metabolism is still poorly understood. To study the relevant regulatory network, fifty‐eight FA biosynthesis genes including de novo synthases, desaturases and elongases were selected as “guide genes” to construct the co‐expression network. Calculation of the correlation between all Arabidopsis thaliana (L.) genes with each guide gene by Arabidopsis co‐expression dating mining tools (ACT) identifies 797 candidate FA‐correlated genes. Gene ontology (GO) analysis of these co‐expressed genes showed they are tightly correlated to photosynthesis and carbohydrate metabolism, and function in many processes. Interestingly, 63 transcription factors (TFs) were identified as candidate FA biosynthesis regulators and 8 TF families are enriched. Two TF genes, CRC and AP1, both correlating with 8 FA guide genes, were further characterized. Analyses of the ap1 and crc mutant showed the altered total FA composition of mature seeds. The contents of palmitoleic acid, stearic acid, arachidic acid and eicosadienoic acid are decreased, whereas that of oleic acid is increased in ap1 and crc seeds, which is consistent with the qRT‐PCR analysis revealing the suppressed expression of the corresponding guide genes. In addition, yeast one‐hybrid analysis and electrophoretic mobility shift assay (EMSA) revealed that CRC can bind to the promoter regions of KCS7 and KCS15, indicating that CRC may directly regulate FA biosynthesis.
Perilipin‐1 in Hemodialyzed Patients: Association With History of Coronary Heart Disease and Lipid Profile
Authors: Eleftheriadis Theodoros , Antoniadi Georgia , Liakopoulos Vassilios , Pissas Georgios , Arampatzis Spyridon , Sparopoulou Theodora , Galaktidou Grammati , Stefanidis Ioannis 
Published: June 2012

AbstractPerilipin‐1 surrounds lipid droplets in both adipocytes and in atheroma plaque foam cells and controls access of lipases to the lipid core. In hemodialysis (HD) patients, dyslipidemia, malnutrition, inflammation and atherosclerosis are common. Thirty‐six HD patients and 28 healthy volunteers were enrolled into the study. Ten HD patients suffered from coronary heart disease (CHD). Perilipin‐1, triglycerides, total cholesterol, low‐density lipoprotein cholesterol, high‐density lipoprotein cholesterol (HDL‐C), body mass index, albumin, geriatric nutritional risk index, normalized protein catabolic rate, interleukin‐6 (IL‐6) and tumor necrosis factor‐α (TNF‐α) were measured. Perilipin‐1 did not differ between HD patients and healthy volunteers. IL‐6 and TNF‐α were higher in HD patients. The evaluated nutritional markers and the markers of inflammation did not differ between HD patients with high perilipin‐1 levels and HD patients with low perilipin‐1 levels. Regarding the lipid profile, only HDL‐C differed between HD patients with high perilipin‐1 levels and HD patients with low perilipin‐1 levels, and it was higher in the first subgroup. Perilipin‐1 was significantly higher in HD patients without CHD. Perilipin‐1 is detectable in the serum of HD patients and it is associated with increased HDL‐C and decreased incidence of CHD.