bluuebunny/crossref_metadata_2025_split huggingface dataset:
Positive patch test reactions to gentamicin show sensitization to aminoglycosides from topical therapies, bone cements, and from systemic medication
Authors: Liippo Jussi , Lammintausta Kaija 
Published: August 2010

Background:  A history of prolonged use of topical antimicrobials is common among patients with positive patch test reactions to gentamicin and to aminoglycosides.Objectives:  The aim of this study was to show sources of gentamicin sensitization in patients with positive patch test reactions to gentamicin.Patients and methods:  About 7814 patients were patch tested with a baseline patch test series and 620 of them were further tested with gentamicin. The clinical histories, concurrent contact sensitivities, and sources of sensitization are analysed among these patients.Results:  Positive patch test reactions to gentamicin were seen in 29/620 patients, most of whom (18/29) also reacted to neomycin and to kanamycin (7/29). Mean age of the gentamicin‐positive patients was 62 years, but three young operating room nurses with hand dermatitis had a history of gentamicin exposure from bone cement. Among the 11/29 neomycin‐negative patients, a history of exposure to different aminoglycosides was apparent, and one patient had a history of systemic netilmicin‐medication‐associated exanthema.Conclusions:  Positive patch test reactions to gentamicin reflect sensitization to different aminoglycosides for which gentamicin seems to represent a sensitive indicator. Gentamicin sensitization may result from occupational exposure to gentamicin containing bone cements or from systemic medication with aminoglycosides.
Can simultaneous contact allergies to phenyl glycidyl ether and epoxy resins of the bisphenol A/F‐types be explained by contamination of the epoxy resins?
Authors: Pontén Ann , Zimerson Erik , Bruze Magnus 
Published: August 2010

Background:  Simultaneous contact allergies to epoxy resins based on diglycidyl ether of bisphenol A (DGEBA‐R) or epoxy resins of the bisphenol F‐type and the reactive diluent phenyl glycidyl ether (PGE) have been reported. The reason might be cross‐reactivity, exposure to an epoxy resin system with PGE as a component, or contamination by PGE in the epoxy resin.Aims:  To study contamination by PGE, 20 commercial epoxy resins were analysed for the presence of PGE. To study contact allergy to PGE and its relation to epoxy resins by inserting PGE in the standard series.Results:  Among 2227 patients, 7 reacted to PGE. Of 23 (30%) patients, 7 with contact allergy to DGEBA‐R and 7/19 (37%) with contact allergy to an epoxy resin of the bisphenol F‐type reacted to PGE. All 7 patients with contact allergy to PGE reacted both to the DGEBA‐R and to the epoxy resin of the bisphenol F‐type. PGE was found in 90% of the investigated resins. The amounts of PGE ranged between 0.004% w/w and 0.18% w/w.Conclusion:  Most probably, the presence of PGE as a contaminant in epoxy resins is of minor importance for the sensitization, but possibly the contamination of PGE might elicit contact dermatitis in individuals with a high reactivity to PGE.
Analysis of allergens in metalworking fluids
Authors: Henriks‐Eckerman Maj‐Len , Suuronen Katri , Jolanki Riitta 
Published: August 2010

Background:  Metalworking fluids (MWFs) are well‐known causes of occupational contact dermatitis in machinists.Objective:  To gain information about skin sensitizers in MWFs and to compare it with the information in safety data sheets (SDSs).Methods:  A total of 17 samples of MWF concentrates were analysed for skin sensitizers known or suspected to be used in MWF. Alkanolamines, formaldehyde, isothiazolinones, methyldibromo glutaronitrile (MDBGN), and iodopropynyl butylcarbamate (IPBC) were separated by liquid chromatography. Resin acids of colophonium (colophony) were separated by gas chromatography. The substances were identified with mass spectrometric detection and ultraviolet detection.Results:  Of the MWFs, 15 contained 6–39% of alkanolamines, mostly monoethanolamine and triethanolamine. Formaldehyde was detected in all MWFs: the concentrations of total formaldehyde ranged between 0.002% and 1.3%. Benzisothiazolinone and octylisothiazolinone were detected in one fluid each. IPBC was detected in nine MWFs, and the highest concentration was 0.09%. Methylisothiazolinone and MDBGN were not detected in any of the fluids. Resin acids of colophonium were detected in seven MWFs in concentrations ranging from 0.41% to 3.8%. On the whole, the allergens analysed were poorly declared in the SDSs.Conclusions:  The content of total formaldehyde was not declared in any SDS. IPBC, a relatively new allergen, seems to be common in MWFs. Isothiazolinones may be relevant allergens of machinists, and they should be analysed in MWFs in case other sources are not identified. The occupational relevance of positive patch test results to MWF ingredients in machinists is difficult to determine if information in the SDSs is relied upon.
Paraffin section immunohistochemistry as an adjunct to morphologic analysis in the diagnosis of cutaneous lymphoid infiltrates*
Authors: Ritter Jon H. , Adesokan Paula N. , Fitzgibbon James F. , Wick Mark R. 
Published: August 2010

In the skin, separation of selected lymphomas from lymphoid hyperplasia can be challenging. The authors examined 45 cutaneous lymphomas, excluding mycosis fungoides (26 small lymphocytic or mixed tumors; 19 large‐cell lymphomas), 10 “atypical” lesions, and 40 lesions of presumed lymphoid hyperplasia, comparing morphologic attributes of such proliferations with their immunophenotypes in paraffin sections. The object of this study was to determine whether immunologic data obtained from routinely‐processed specimens could be used to further objectify morphologic interpretations. Features favoring lymphonia included a lesional epicenter in the lower clermis or subcutis; poor circumscription of lymphoid aggregates; and dissection of lymphoid cells between collagen bundles. Immunostains included antibodies to CD20, CD43, CD45, CD45RO, CD45RA, CD68, proliferating cell nuclear antigen (PCNA), and MB2. Eleven of 26 small lymphocytic or mixed‐cell lymphomas and 3 of 10 “atypical” cases demonstrated an abnormal immunophenotype, including co‐expression of CD43 and CD20 or non‐physiological CD45RA distribution. In contrast, none of 40 cases with benign features manifested aberrant antigen expression. Thirty‐one of 37 cases in which 75% of the cells typed as B lymphocytes showed malignant morphologic features, 5 were “atypical” and possibly lymphomatous, and only one had benign features. PCNA stains showed greater positivity of the lymphoid nuclei in Lymphomas, and a labeling index of <30% was correlated with malignancy in this context. These observations indicate that immunostaining may provide useful adjunctive information in distinguishing benign from malignant cutaneous lymphoid proliferations in paraffin sections.Ritter JH, Adesokan PN, Fitzgibbons JF, Wick MR. Paraffin section immunohistochemistry as an adjunct to morphologic analysis in the diagnosis of cutaneous lymphoid infiltrates.
Cell‐type related and spatial variation in the expression of integrins in cutaneous tumors
Authors: Tuominen Hannu , Junttila Taina , Karvonen Jaakko , Kallioinen Matti 
Published: August 2010

Integrins constitute a group of transmembrane proteins which mediate cell‐cell and cell‐matrix interactions. Previous studies have shown both increased and decreased expression of integrins in relation to malignancy and invasion. In the present study, we investigated integrin distribution in cutaneous tumors by using monoclonal antibodies on frozen tissue sections. Antibodies to integrin subunits αv, α3, α4, α5, α6, β1 and β3 were used. The study was designed to explore (i) the association between integrin expression and the tumor type, and (ii) the effect on the integrin expression of the location of the tumor, i.e. whether it grows intraepidermally or within various compartments of the dermis (papillary or reticular). (β1, β3 and α3 were strongly or moderately expressed in the epithelial and stromal cells of basal cell carcinomas (BCC), seborrheic keratoses, solar keratoses, dermatofibromas (DF), and showed a variable expression in the nevic cells of benign and dysplas‐tic nevocellular nevi. αv and in α5 appeared strongly expressed in the stromal cells of BCC and DF, while only a focal, often weak staining was seen in nevic cells and in the epithelial cells of BCCs. In some nevocellular nevi, they were only expressed, together with α4, in the deep‐seated nevic cells in the reticular dermis. aL6 was expressed by minor cells of BCCs and nevocellular nevi only within the dermo‐epidermal junction. In seborrheic keratosis and solar keratosis a basement membrane‐associated staining pattern for α6 was seen in the basal cell layer, with focal discontinuities in solar keratosis. In conclusion, the results indicate a wide expression of various types of integrins in cutaneous tumors. The expression is distinctly tumor‐type and cell‐type dependent but is also influenced e.g. by the depth of the stromal infiltration in BCC (α6) and in nevocellular tumors (αv, 4, 5, 6, β3), probably reflecting adaptation to the variable microenvironmental influences.Tuominen H, Junttila T, Karvonen J, Kallioinen M. Cell‐type related and spatial variation in the expression of integrins in cutaneous tumors.
The expression of microRNA‐203 during human skin morphogenesis
Authors: Wei Tianling , Orfanidis Kyriakos , Xu Ning , Janson Peter , Ståhle Mona , Pivarcsi Andor , Sonkoly Enikö 
Published: August 2010

Please cite this paper as: The expression of microRNA‐203 during human skin morphogenesis. Experimental Dermatology 2010; 19: 854–856.Abstract:  MicroRNAs are small, non‐coding RNAs that regulate gene expression post‐transcriptionally and play important roles in various biological processes. We previously identified miR‐203 as a skin‐ and keratinocyte‐specific microRNA. Moreover, miR‐203 has been implicated in repressing ‘stemness’ in epidermal progenitors. Here, we investigate the expression of miR‐203 and two of its targets, p63 and suppressor of cytokine signalling‐3, during human skin morphogenesis. MiR‐203 in situ hybridization was performed on sections of human foetal skin ranging from 14 to 22 weeks’ gestation and adult skin. MiR‐203 was barely detectable at 14 weeks. Its expression became prominent from week 17 and was most pronounced in the suprabasal layers of the epidermis, while p63 and SOCS‐3 were preferentially expressed in the basal layer. Differentiation markers such as involucrin and filaggrin were expressed mainly in the suprabasal layers of epidermis, similar to miR‐203. Our results support the involvement of miR‐203 in skin morphogenesis.
Multiple myeloma: a study of 10 cases
Authors: Raubenheimer E. J. , Dauth J. , van Wilpe E. 
Published: August 2010

Raubenheimer EJ, Dauth J, Van Wilpe E. Multiple myeloma: a study of 10 cases. J Oral Pathol 1987: 16: 383–388.The clinical, radiographical, biochemical, microscopical and ultrastructural features of 10 cases of multiple myeloma were studied. The skull and jaw regions were frequently involved by the disease and although the diagnosis remains multidisciplinary, microscopical parameters differentiating a myelomatous bone marrow infiltrate from a reactive plasmacytosis are discussed. Biochemical and microscopical factors influencing the prognosis are highlighted and significant ultrastructural findings include erythrophagocytosis, cytoplasmic nuclear asynchrony of plasmablasts and intranuclear viral like inclusions.
Salivary peroxidase: an important part of our defense against oxygen toxicity
Authors: Carlsson J. 
Published: August 2010

Carlsson J. Salivary peroxidase: an important part of our defense against oxygen toxicity. J Oral Pathol 1987: 16: 412–416.All living cells convert dioxygen (O2) into the toxic intermediates of oxygen reduction: superoxide radicals, hydrogen peroxide and hydroxyl radicals. The defense against these intermediates works on three levels: 1) the formation of the intermediates is prevented; 2) the intermediates are scavenged; 3) the damaged sites are repaired. Various parts of this defense are found intracellular^, in tissue fluid, in blood plasma and in the external secretions. In the mouth there is a special need for defense against hydrogen peroxide, because hydrogen peroxide is not only formed by bacteria colonizing the mucous membranes but also by the cells of the salivary glands. In saliva the most important part of this defense is salivary peroxidase, which detoxifies hydrogen peroxide in the presence of thiocyanate by converting it into hypothiocyanite, dioxygen and water. In addition, hypothiocyanite stops hydrogen peroxide production by the oral bacteria.
Interactions between peroxide and salivary glycoprotein: protection by peroxidase
Authors: Ericsno T. , Bratt P. 
Published: August 2010

Ericson T, Bratt P. Interactions between peroxide and salivary glycoprotein: protection by peroxidase. J Oral Pathol 1987: 16: 421–424.The effect of ascorbic acid oxidation and of peroxide on salivary glycoproteins has been studied. No signs of depolymerization of the glycoproteins were observed as a result of treatment of whole saliva or the isolated glycoprotein fraction from human saliva with an ascorbic acid, hydrogen peroxide and cupric ion combination. Sialic acid was analyzed with two methods which gave different chromagens of the acid. The conclusion could be drawn from the results that the effect of ascorbic acid oxidation was a decarboxylation of sialic acid in salivary glycoproteins. This also destroys the bacteria‐agglutinating activity of saliva. Full protection of the agglutinin activity is given by salivary peroxidase. It is suggested that one role of peroxidases in saliva is to protect biologically active proteins from the action of oxygen or its radicals.
Overexpression of bcl‐2 protein inhibits terminal differentiation of oral keratinocytes in vitro
Authors: Harada Hidemitsu , Mitsuyasu Takeshi , Seta Yuji , Maruoka Yuka , Toyoshima Kuniaki , Yasumoto Shigeru 
Published: August 2010

Harada H, Mitsuyasu T, Seta Y. Maruoka Y, Toyoshima K, Yasumoto S: Overexpression of bcl‐2 protein inhibits terminal differentiation of oral keratinocytes in vitro. J Oral Pathol Med 1998; 27: 11–7. © Munksgaard, 1998.The bcl‐2 proto‐oncogene is a known inhibitor of apoptosis; in normal human stratified squamous epithelium, its expression is restricted to the basal cell layer. To investigate the functional role of bcl‐2 protein in the process of differentiation of oral keratinocytes, bcl‐2 expression vector was transfected into SCC‐25 cells, which normally undergo squamous cell differentiation in vitro while expressing specific differentiation markers, e.g., keratin 10/11 and involucrin. In bcl‐2 transfected SCC‐25 cells, the expression of these differentiation markers was markedly suppressed. The bcl‐2 proto‐oncogene may play a critical role in opposing the commitment to terminal differentiation and apoptosis of oral keratinocytes.
Ameloblastomas in cats: a critical evaluation of the literature and the addition of one example
Authors: Gardner David G. 
Published: August 2010

Gardner DG: Ameloblastomas in cats: a critical evaluation of the literature and the addition of one example. J Oral Pathol Med 1998; 27: 39–42. © Munks‐gaard, 1998.The English language literature on feline ameloblastomas was evaluated. Seven acceptable cases were found and one additional example is added in this article. These rare tumors have occurred in cats over the age of 6 years, affected both sexes, both jaws, and various breeds. Two microscopic variants have so far been reported, namely the follicular pattern and the keratinizing pattern, which is similar to the keratinizing ameloblastoma in dogs. Ameloblastomas in cats have been confused in the past with two other feline odontogenic tumors: the inductive fibro‐ameloblastoma (feline inductive odontogenic tumor) and the “calcifying epithelial odontogenic tumor” (amyloid‐producing odontogenic tumor).
Proliferative activity in peripheral ossifying fibroma and ossifying fibroma
Authors: Mesquita Ricardo Alves , Orsini Suzana Catanhede , Sousa Machado , de Araujo Ney Soares 
Published: August 2010

Mesquita RA, Sousa SCOM, Araujo NS: Proliferative activity in peripheral ossifying fibroma and ossifying fibroma. J Oral Pathol Med 1998; 27: 64–7. © Munksgaard, 1998.A proliferative activity study analysing morphometric and quantitative aspects of nucleolar organizer regions (NORs) and proliferating cell nuclear antigen (PCNA) expression was conducted in 10 cases of peripheral ossifying fibroma (POF) and 10 cases of ossifying fibroma (OF). For NOR identification, the silver staining technique (AgNOR technique) was used. PCNA expression was determined by immunohistochemical staining using the PC 10 antibody. The AgNOR analysis for the two lesions showed a profile characteristic of benign lesions. OF showed higher AgNOR number and PCNA expression than POF. Our results suggest increased proliferative activity in OF compared with POF.
Maxillofacial osteonecrosis in a patient with multiple “idiopathic” facial pains
Authors: Adams William R. , Spolnik Kenneth J. , Bouquot Jerry E. 
Published: August 2010

Adams WR, Spolnik KJ, Bouquot JE: Maxillofacial osteonecrosis in a patient with multiple “idiopathic” facial pains. J Oral Pathol Med 1999; 28: 423–32. © Munksgaard, 1999.Previous investigations have identified focal areas of alveolar bone tenderness, increased mucosal temperature, abnormal anesthetic response, radiographic abnormality, increased radioisotope uptake on bone scans, and abnormal marrow within the quadrant of pain in patients with chronic, idiopathic facial pain. The present case reports a 53‐year‐old man with multiple debilitating, “idiopathic” chronic facial pains, including trigeminal neuralgia and atypical facial neuralgia. At necropsy he was found to have numerous separate and distinct areas of ischemic osteonecrosis on the side affected by the pains, one immediately beneath the major trigger point for the lancinating pain of the trigeminal neuralgia. This disease, called NICO (neuralgia‐inducing cavitational osteonecrosis) when the jaws are involved, is a variation of the osteonecrosis that occurs in other bones, especially the femur. The underlying problem is vascular insufficiency, with intramedullary hypertension and multiple intraosseous infarctions occurring over time. The present case report illustrates the extreme difficulties involved in the diagnosis and treatment of this disease.
Oral histoplasmosis associated with HIV infection: a comparative study
Authors: Hernández S. L. , López de Blanc S. A. , Sambuelli R. H. , Roland H. , Cornelli C. , Lattanzi V. , Carnelli M. A. 
Published: August 2010

Objective:  Histoplasmosis is a granulomatous fungal disease caused by Histoplasma capsulatum. The objective of the present paper was to describe the prevalence of oral histoplasmosis (OH) in two services from an endemic area in Argentina between 1991 and 2002 and to compare the clinicopathological profile of OH between HIV‐positive and HIV‐negative patients.Methods:  About 733 HIV+ (group A) and 14 260 patients (group B) were examined. Clinical diagnosis was confirmed by cytology, biopsy or culture.Results:  About 21 (3%) and 10 (0.07%) cases of OH were diagnosed in group A and B respectively. Most patients were male. A total of 90% of patients in group A were <45 years old whereas 70% of group B were more than 45 years old. Palate, gingiva and oropharynx were the most frequent locations. The importance of including histoplasmosis in the differential diagnosis of ulcerated oral lesions in immunocompromised patients was discussed.
Extracellular glycosaminoglycan changes in healthy and overgrown gingiva fibroblasts after cyclosporin A and cytokine treatments
Authors: Stabellini G. , Calastrini C. , Mariani G. , Gioia M. , Caramelli E. , Gagliano N. , Dellavia C. , Moscheni C. , Vizzotto L. , Calvi E. M. 
Published: August 2010

Background:  It has been demonstrated that cyclosporin A (CyA) blocks the immune system, acts on cytoskeleton and stimulates the production of extracellular matrix (ECM) and transforming growth factor‐β1 (TGF‐β1). This cytokine, such as transforming growth factor‐α (TGF‐α), induces deposition of glycosaminoglycans (GAG), proteoglycans and collagen fibres in the ECM.Methods:  In this work, we examined the effect induced by CyA, TGF‐β1 and TGF‐α on cultures of healthy and overgrown human gingival fibroblasts in order to evaluate the glycosaminoglycan, cytoskeletal changes and the behaviour of fibroblasts after concanavalin A (Con A) treatment. Moreover, we examined gingival biopsies by Alcian blue histochemical staining and electron transmission microscopy.Results:  Total and extracellular sulphated GAG in overgrown gingiva specimens and in derived fibroblast cultures treated with CyA and cytokines were significantly higher than controls. The action of cytokines was increased (P ≤ 0.01) compared with CyA with a greater effect of TGF‐α in comparison with TGF‐β1; the electron microscopy showed ECM accumulation. The agglutinations showed the heterogeneity of fibroblast populations.Conclusions:  Stimulation with Con A showed that the fibroblast population had cell surface heterogeneity, and could respond in a different way to both CyA and cytokine stimulus. Moreover, increased synthesis of GAG in overgrown gingiva compared with synthesis in normal fibroblasts before CyA treatment suggests a possible genetic origin of damage. As not all CyA‐treated patients develop gingival overgrowth, a genetic predisposition may explain the different responses of gingival fibroblast populations.
Unsuspected small ameloblastoma in the alveolar bone: a collaborative study of 14 cases with discussion of their cellular sources
Authors: Ide F. , Mishima K. , Yamada H. , Horie N. , Saito I. , Shimoyama T. , Kusama K. 
Published: August 2010

Background:  Intraosseous ameloblastoma (IA) is the quintessence of epithelial odontogenic tumor and histologically and behaviorally defined as an undoubted neoplastic process. Current information must lead to the consensus that IA arises from the embryologic inclusions of odontogenic epithelium within the jawbone. Nevertheless, clinically oriented evidence is limited to this day.Methods:  The clinical and radiographic features, behavior, and pathology of 14 cases of small IA confined to the alveolar region were systematically examined.Results:  Six cases were a chance finding. There was no gender predilection and half of the lesions clustered in middle age (>40 years). The posterior region of the mandible (n = 7) and the anterior segment of the maxilla (n = 4) were favored. Five radiographic characteristics were recognized: interradicular (n = 5) and periradicular (n = 3), and periapical, residual and pericoronal (n = 2 each). They showed solid (n = 12) or unicystic (n = 2) growth pattern and 12 lesions were divided into seven follicular, three desmoplastic, and two plexiform subtypes. The main location of tumor was microscopically traceable in six cases; three interradicular type outside the periodontal ligament space and two periradicular and one periapical variants inside.Conclusion:  By in‐depth evaluation of the spatial relationship between tumor and its surrounding structure, the alveolar process, periodontal ligament space, and pericoronal area are all the likely starting points of IA. This report re‐awakens the oral pathologist to the histogenetic significance of incipient IA as the only available human specimen for reappraisal of their origin.