bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thermal Optimization Strategies for Li-Ion Batteries: Predictive Temperature Algorithm
Authors: Antonio Metallo 
Published: May 2024

Abstract
Performance, safety, and longevity of batteries are all strongly impacted by thermal management, which is an essential component of battery design and operation. This work examines how accurate temperature control can result in significant improvements in performance and reliability with a focus on battery thermal heating. Predicting the temperature achieved by the battery during operation not only avoids conditions that lead to thermal runaway but also guarantees that the battery is used optimally within an optimal temperature range. Within the optimal temperature range, several advantages are observed. First, battery efficiency improves significantly as electrochemical processes occur more efficiently. Furthermore, by lowering the possibility of short circuits and improving overall battery safety, thermal stability aids in the prevention of undesirable phenomena like dendrite growth. By lessening the deterioration brought on by thermal degradation processes, thermal optimization also affects battery longevity. Based on experimental tests, a finite element method (FEM) model is developed. A model for thermal runaway propagation is established by combining thermal runaway and conduction models with an Arrhenius law-based combustion model. The study employed a cylindrical Li-ion cell to conduct tests, taking into account three parameters: discharge rate (CRate), ambient temperature (Tamb), and initial battery temperature (T0). An algorithm based on the three variables was developed using the simulation results. The algorithm enables the accurate prediction of rising battery temperature during use, facilitating the setting of an optimal maximum discharge rate considering initial and ambient temperatures, thereby ensuring optimal performance within the desired temperature range.
Validation of aminodeoxychorismate synthase and anthranilate synthase as novel targets for bispecific antibiotics inhibiting conserved protein-protein interactions
Authors: Funke Franziska Jasmin , Schlee Sandra , Sterner Reinhard 
Published: May 2024

ABSTRACT

Multi-resistant bacteria are a rapidly emerging threat to modern medicine. It is thus essential to identify and validate novel antibacterial targets that promise high robustness against resistance-mediating mutations. This can be achieved by simultaneously targeting several conserved function-determining protein-protein interactions in enzyme complexes from prokaryotic primary metabolism. Here, we selected two evolutionary related glutamine amidotransferase complexes, aminodeoxychorismate synthase and anthranilate synthase, that are required for the biosynthesis of folate and tryptophan in most prokaryotic organisms. Both enzymes rely on the interplay of a glutaminase and a synthase subunit that is conferred by a highly conserved subunit interface. Consequently, inhibiting subunit association in both enzymes by one competing bispecific inhibitor has the potential to suppress bacterial proliferation. We comprehensively verified two conserved interface hot-spot residues as potential inhibitor-binding sites
in vitro
by demonstrating their crucial role in subunit association and enzymatic activity. For
in vivo
target validation, we generated genomically modified
Escherichia coli
strains in which subunit association was disrupted by modifying these central interface residues. The growth of such strains was drastically retarded on liquid and solid minimal medium due to a lack of folate and tryptophan. Remarkably, the bacteriostatic effect was observed even in the presence of heat-inactivated human plasma, demonstrating that accessible host metabolite concentrations do not compensate for the lack of folate and tryptophan within the tested bacterial cells. We conclude that a potential inhibitor targeting both enzyme complexes will be effective against a broad spectrum of pathogens and offer increased resilience against antibiotic resistance.

IMPORTANCE

Antibiotics are indispensable for the treatment of bacterial infections in human and veterinary medicine and are thus a major pillar of modern medicine. However, the exposure of bacteria to antibiotics generates an unintentional selective pressure on bacterial assemblies that over time promotes the development or acquisition of resistance mechanisms, allowing pathogens to escape the treatment. In that manner, humanity is in an ever-lasting race with pathogens to come up with new treatment options before resistances emerge. In general, antibiotics with novel modes of action require more complex pathogen adaptations as compared to chemical derivates of existing entities, thus delaying the emergence of resistance. In this contribution, we use modified
Escherichia coli
strains to validate two novel targets required for folate and tryptophan biosynthesis that can potentially be targeted by one and the same bispecific protein-protein interaction inhibitor and promise increased robustness against bacterial resistances.
Complete genome sequence of iron-oxidizing
Stutzerimonas stutzeri
strain FeN3W isolated from Catalina Harbor sediment
Authors: Yu Jin-Sang , Rowe Annette R. , Sackett Joshua D. 
Published: May 2024

ABSTRACT

Stutzerimonas stutzeri
strain FeN3W is an iron-oxidizing bacterium isolated from marine sediment. FeN3W’s 5.9 Mb genome encodes complete pathways for glycolysis, gluconeogenesis, TCA cycle, pentose phosphate pathway, and aerobic and anaerobic (nitrate) respiration. The genome contains 32 putative heme-binding proteins predicted to localize to the cell envelope.
Complete genome sequence of
Delftia tsuruhatensis
strain HA60 isolated from a commercial hydroxyapatite nano-particle product (nano-hydroxyapatite)
Authors: Wang Sharon X. , Park Miseon , Kim Sung Guk 
Published: May 2024

ABSTRACT

Delftia tsuruhatensis is
a Gram-negative rod-shaped aerobic bacterium with environmental remediation functions.
D. tsuruhatensis
strain HA60 was isolated from a commercial nano-particle product, nano-hydroxyapatite. We report that the genome of
D. tsuruhatensis
strain HA60 has a circular genome of 6,922,195 base pairs with a G+C content of 66.45%.
Genome sequence, antibiotic resistance genes, and plasmids in a monophasic variant of
Salmonella typhimurium
isolated from retail pork
Authors: Ghorbani Tajani Anahita , Sharma Aniket , Blouin Nicolas , Bisha Bledar 
Published: May 2024

ABSTRACT

A
Salmonella
isolate from retail pork was whole genome sequenced using Illumina NovaSeq6000, with a 5,320,119 bp genome and 51.06% GC content. Several antibiotic resistance genes and plasmids, including
bla
TEM-1
,
aac(6')-IIc
, IncHI2, and p0111 were obtained from subsequent analysis. These findings provide vital insights into generic determinants of antimicrobial resistance (AMR) in this foodborne pathogen.
Fusion/fission protein family identification in Archaea
Authors: Padalko Anastasiia , Nair Govind , Sousa Filipa L. 
Published: May 2024

ABSTRACT

The majority of newly discovered archaeal lineages remain without a cultivated representative, but scarce experimental data from the cultivated organisms show that they harbor distinct functional repertoires. To unveil the ecological as well as evolutionary impact of
Archaea
from metagenomics, new computational methods need to be developed, followed by in-depth analysis. Among them is the genome-wide protein fusion screening performed here. Natural fusions and fissions of genes not only contribute to microbial evolution but also complicate the correct identification and functional annotation of sequences. The products of these processes can be defined as fusion (or composite) proteins, the ones consisting of two or more domains originally encoded by different genes and split proteins, and the ones originating from the separation of a gene in two (fission). Fusion identifications are required for proper phylogenetic reconstructions and metabolic pathway completeness assessments, while mappings between fused and unfused proteins can fill some of the existing gaps in metabolic models. In the archaeal genome-wide screening, more than 1,900 fusion/fission protein clusters were identified, belonging to both newly sequenced and well-studied lineages. These protein families are mainly associated with different types of metabolism, genetic, and cellular processes. Moreover, 162 of the identified fusion/fission protein families are archaeal specific, having no identified fused homolog within the bacterial domain. Our approach was validated by the identification of experimentally characterized fusion/fission cases. However, around 25% of the identified fusion/fission families lack functional annotations for both composite and split states, showing the need for experimental characterization in Archaea.

IMPORTANCE

Genome-wide fusion screening has never been performed in
Archaea
on a broad taxonomic scale. The overlay of multiple computational techniques allows the detection of a fine-grained set of predicted fusion/fission families, instead of rough estimations based on conserved domain annotations only. The exhaustive mapping of fused proteins to bacterial organisms allows us to capture fusion/fission families that are specific to archaeal biology, as well as to identify links between bacterial and archaeal lineages based on cooccurrence of taxonomically restricted proteins and their sequence features. Furthermore, the identification of poorly characterized lineage-specific fusion proteins opens up possibilities for future experimental and computational investigations. This approach enhances our understanding of Archaea in general and provides potential candidates for in-depth studies in the future.
Role of Gut Microbe Composition in Psychosocial Symptom Response to Exercise Training in Breast Cancer Survivors (ROME) study: protocol for a randomised controlled trial
Authors: Little Rebecca B , Carter Stephen J , Motl Robert W , Hunter Gary , Cook Abby , Liu Nianjun , Krontiras Helen , Lefkowitz Elliot J , Turan Bulent , Schleicher Erica , Rogers Laura Q 
Published: May 2024

IntroductionBreast cancer survivors have an increased risk for chronic fatigue and altered gut microbiota composition, both with negative health and quality of life affects. Exercise modestly improves fatigue and is linked to gut microbial diversity and production of beneficial metabolites. Studies suggest that gut microbiota composition is a potential mechanism underlying fatigue response to exercise. Randomised controlled trials testing the effects of exercise on the gut microbiome are limited and there is a scarcity of findings specific to breast cancer survivors. The objective of this study is to determine if fitness-related modifications to gut microbiota occur and, if so, mediate the effects of aerobic exercise on fatigue response.Methods and analysisThe research is a randomised controlled trial among breast cancer survivors aged 18–74 with fatigue. The primary aim is to determine the effects of aerobic exercise training compared with an attention control on gut microbiota composition. The secondary study aims are to test if exercise training (1) affects the gut microbiota composition directly and/or indirectly through inflammation (serum cytokines), autonomic nervous system (heart rate variability) or hypothalamic-pituitary-adrenal axis mediators (hair cortisol assays), and (2) effects on fatigue are direct and/or indirect through changes in the gut microbiota composition. All participants receive a standardised controlled diet. Assessments occur at baseline, 5 weeks, 10 weeks and 15 weeks (5 weeks post intervention completion). Faecal samples collect the gut microbiome and 16S gene sequencing will identify the microbiome. Fatigue is measured by a 13-item multidimensional fatigue scale.Ethics and disseminationThe University of Alabama at Birmingham Institutional Review Board (IRB) approved this study on 15 May 2019, UAB IRB#30000320. A Data and Safety Monitoring Board convenes annually or more often if indicated. Findings will be disseminated in peer-reviewed journals and conference presentations.Trial registration numberClinicalTrials.gov,NCT04088708.
How effective is topical miconazole or amorolfine for mild to moderately severe onychomycosis in primary care: the Onycho Trial – a randomised double-blind placebo-controlled trial
Authors: Watjer Roeland M , Bonten Tobias N , Sayed Khisraw , Quint Koen D , van der Beek Martha T , Mertens Bart J A , Numans Mattijs E , Eekhof Just A H 
Published: May 2024

ObjectivesTo evaluate the efficacy of topical miconazole or amorolfine compared to placebo for mild to moderately severe onychomycosis.DesignRandomised, double-blind, placebo-controlled trial, with computer-generated treatment allocation at a 1:1:1 ratio.SettingPrimary care, recruitment from February 2020 to August 2022.Participants193 patients with suspected mild to moderately severe onychomycosis were recruited via general practices and from the general public, 111 of whom met the study criteria. The mean age of participants was 51 (SD 13.1), 51% were female and onychomycosis was moderately severe (mean OSI 12.1 (SD 8.0)).InterventionsOnce-daily miconazole 20 mg/g or once-weekly amorolfine 5% nail lacquer solution was compared with placebo (denatonium benzoate solution).Main outcome measuresComplete, clinical and mycological cure at 6 months. Secondary outcomes were clinical improvement, symptom burden, quality of life, adverse effects, compliance, patient-perceived improvement and treatment acceptability.ResultsBased on intention-to-treat analysis, none of the participants receiving miconazole or amorolfine reached complete cure compared with two in the placebo group (OR not estimable (n.e.), p=0.493 and OR n.e., p=0.240, respectively). There was no evidence of a significant difference between groups regarding clinical cure (OR n.e., p=0.493 and OR 0.47, 95% CI 0.04 to 5.45, p=0.615) while miconazole and amorolfine were less effective than placebo at reaching both mycological cure (OR 0.25, 95% CI 0.06 to 0.98, p=0.037 and OR 0.23, 95% CI 0.06 to 0.92, p=0.029, respectively) and clinical improvement (OR 0.26, 95% CI 0.08 to 0.91, p=0.028 and OR 0.25, 95% CI 0.07 to 0.85, p=0.021, respectively). There was no evidence of a significant difference in disease burden, quality of life, adverse reactions, compliance, patient-perceived improvement or treatment acceptability.ConclusionsTopical miconazole and amorolfine were not effective in achieving a complete, clinical or mycological cure of mild to moderately severe onychomycosis, nor did they significantly alleviate the severity or symptom burden. These treatments should, therefore, not be advised as monotherapy to treat onychomycosis.Trial registration numberWHO ICTRP NL8193.
Factors influencing the outcomes of non-pharmacological interventions for managing fatigue across the lifespan of people living with musculoskeletal (MSK) conditions: a scoping review protocol
Authors: Fishpool Katie , Young George , Ciurtin Coziana , Cramp Fiona , Erhieyovwe Emmanuel Oghenetejiri , Farisogullari Bayram , Macfarlane Gary J , Machado Pedro M , Pearson Jen , Santos Eduardo , Dures Emma 
Published: May 2024

IntroductionFatigue is an important and distressing symptom for many people living with chronic musculoskeletal (MSK) conditions. Many non-pharmacological interventions have been investigated in recent years and some have been demonstrated to be effective in reducing fatigue and fatigue impact, however, there is limited guidance for clinicians to follow regarding the most appropriate management options. The objective of this scoping review is to understand and map the extent of evidence in relation to the factors that relate to the outcome of non-pharmacological interventions on MSK condition-related fatigue across the lifespan.Methods and analysisThis scoping review will include evidence relating to people of all ages living with chronic MSK conditions who have been offered a non-pharmacological intervention with either the intention or effect of reducing fatigue and its impact. Databases including AMED, PsycINFO, CINAHLPlus, MEDLINE, EMBASE and Scopus will be searched for peer-reviewed primary research studies published after 1 January 2007 in English language. These findings will be used to identify factors associated with successful interventions and to map gaps in knowledge.Ethics and disseminationEthical approval was not required for this review. Findings will be disseminated by journal publications, conference presentations and by communicating with relevant healthcare and charity organisations.
On macroscopic residual QCD force of electrodynamics
Authors: Spousta Martin 
Published: May 2024

AbstractWe explore a connection between virtual particles of quantum electrodynamics and quantum chromodynamics (QCD) which is predicted to give rise to a residual attractive interaction measurable as a macroscopic force. We calculate the asymptotic behavior of relevant scattering amplitudes, perform their resummation, and analyze the sign of the resulting interaction. Then, we calculate the primary experimentally observable consequences of this Standard Model force. We discuss the impact of this force at terrestrial scales and at astrophysical scales. In particular, we quantify the impact of this force on the warm ionized medium present in galaxies and the intracluster medium present in cluster of galaxies.
Diagenetic Products, Settings and Evolution of the Presalt Succession in the Northern Campos Basin, Brazil
Authors: Strugale Michael , Lima Bruno Eustáquio Moreira , Day Christopher , Omma Jenny , Rushton Jeremy , Olivito João Paulo Roncolatto , Bouch Jon , Robb Laurence , Roberts Nick , Cartwright Joe 
Published: May 2024

Abstract
Despite all the effort made towards an understanding of the sedimentary, tectonic, and diagenetic evolution of the presalt sequence and the Pre-Salt reservoirs of the North Campos Basin (East Brazil), two knowledge gaps have yet to be filled: 1) a detailed study of diagenesis in the crystalline basement and rift phases, and 2) the timing of diagenetic events that affected the presalt succession. In this study, samples from these geologic units were analysed for mineral composition and paragenetic evolution, fluid temperature and salinity, stable isotope compositions, and LA-ICP-MS derived U-Pb ages of carbonate phases. The U-Pb ages of replacive and vein-filling cements reveal three tectono-diagenetic events, named Barremian-Aptian (BADE, 125-117 Ma), Albo-Cenomanian (ACDE, 103-98 Ma), and Campanian-Maastrichtian (CMDE, 83-70 Ma). Each phase is characterised by distinct minerals, precipitation temperatures, and burial conditions. The hydrothermal qualifier, identified by the temperature contrast between fluid and host rock, was initially high during BADE, then diminished over time (through ACDE) until it achieved equilibrium with the host rocks during CMDE. Diagenetic events are not coeval with magmatism but do coincide with known regional tectonic events described in the literature and are interpreted to be the result of increasing intraplate stresses. Multidisciplinary studies that include diagenetic events constrained by geochronological data will certainly lead to more robust conceptual geologic models, and therefore, to a more reliable management of resources and strategies such as Enhance Oil Recovery (EOR), Carbon Capture, Utilisation and Storage (CCUS), and drinking water.

Supplementary material at
https://doi.org/10.6084/m9.figshare.c.7103900
Association Between Sleep Apnea Treatment and Health Care Resource Use in Patients With Atrial Fibrillation
Authors: Sterling Kimberly L. , Alpert Naomi , Malik Anita S. , Pépin Jean‐Louis , Benjafield Adam V. , Malhotra Atul , Piccini Jonathan P. , Cistulli Peter A. , Nunez Carlos M. , Barrett Meredith , Armitstead Jeff 
Published: May 2024

Background
Obstructive sleep apnea (OSA) contributes to the generation, recurrence, and perpetuation of atrial fibrillation, and it is associated with worse outcomes. Little is known about the economic impact of OSA therapy in atrial fibrillation. This retrospective cohort study assessed the impact of positive airway pressure (PAP) therapy adherence on health care resource use and costs in patients with OSA and atrial fibrillation.

Methods and Results

Insurance claims data for ≥1 year before sleep testing and 2 years after device setup were linked with objective PAP therapy use data. PAP adherence was defined from an extension of the US Medicare 90‐day definition. Inverse probability of treatment weighting was used to create covariate‐balanced PAP adherence groups to mitigate confounding. Of 5867 patients (32% women; mean age, 62.7 years), 41% were adherent, 38% were intermediate, and 21% were nonadherent. Mean±SD number of all‐cause emergency department visits (0.61±1.21 versus 0.77±1.55 [
P
=0.023] versus 0.95±1.90 [
P
<0.001]), all‐cause hospitalizations (0.19±0.69 versus 0.24±0.72 [
P
=0.002] versus 0.34±1.16 [
P
<0.001]), and cardiac‐related hospitalizations (0.06±0.26 versus 0.09±0.41 [
P
=0.023] versus 0.10±0.44 [
P
=0.004]) were significantly lower in adherent versus intermediate and nonadherent patients, as were all‐cause inpatient costs ($2200±$8054 versus $3274±$12 065 [
P
=0.002] versus $4483±$16 499 [
P
<0.001]). All‐cause emergency department costs were significantly lower in adherent and intermediate versus nonadherent patients ($499±$1229 and $563±$1292 versus $691±$1652 [
P
<0.001 and
P
=0.002], respectively).

Conclusions
These data suggest clinical and economic benefits of PAP therapy in patients with concomitant OSA and atrial fibrillation. This supports the value of diagnosing and managing OSA and highlights the need for strategies to enhance PAP adherence in this population.
Behavior of Extracranial‐to‐Intracranial Extended Arterial Dissections of the Vertebral Artery
Authors: Park So Young , Lee Jin Soo , Kim Min , Jung Woo Sang , Choi Jin Wook , Hong Ji Man , Lee Seong‐Joon 
Published: May 2024

Background
Vertebral artery dissections (VADs) may extend from the extracranial to the intracranial vasculature (e+iVAD). We evaluated how the characteristics of e+iVAD differed from those of intracranial VAD (iVAD).

Methods and Results

From 2002 to 2019, among consecutive patients with cervicocephalic dissection, those with iVAD and e+iVAD were included, and their clinical characteristics were compared. In patients with unruptured dissections, a composite clinical outcome of subsequent ischemic events, subsequent hemorrhagic stroke, or mortality was evaluated. High‐resolution magnetic resonance images were analyzed to evaluate intracranial remodeling index. Among 347 patients, 51 (14.7%) had e+iVAD and 296 (85.3%) had iVAD. The hemorrhagic presentation occurred solely in iVAD (0.0% versus 19.3%), whereas e+iVAD exhibited higher ischemic presentation (84.3% versus 27.4%;
P
<0.001). e+iVAD predominantly presented steno‐occlusive morphology (88.2% versus 27.7%) compared with dilatation patterns (11.8% versus 72.3%;
P
<0.001) of iVAD. The ischemic presentation was significantly associated with e+iVAD (iVAD as a reference; adjusted odds ratio, 3.97 [95% CI, 1.67–9.45];
P
=0.002]). Patients with unruptured VAD showed no differences in the rate of composite clinical outcome between the groups (log‐rank,
P
=0.996). e+iVAD had a lower intracranial remodeling index (1.4±0.3 versus 1.6±0.4;
P
<0.032) and a shorter distance from dural entry to the maximal dissecting segment (6.9±8.4 versus 15.7±7.4;
P
<0.001).

Conclusions
e+iVAD is associated with lower rates of hemorrhages and higher rates of ischemia than iVAD at the time of admission. This may be explained by a lower intracranial remodeling index and less deep intrusion of the dissecting segment into the intracranial space.