bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stereotactic body radiation therapy for colorectal liver metastases
Authors: van der Pool A E M , Méndez Romero A , Wunderink W , Heijmen B J , Levendag P C , Verhoef C , IJzermans J N M 
Published: January 2010

Abstract

Background
Stereotactic body radiation therapy (SBRT) is a treatment option for colorectal liver metastases. Local control, patient survival and toxicity were assessed in an experience of SBRT for colorectal liver metastases.

Methods
SBRT was delivered with curative intent to 20 consecutively treated patients with colorectal hepatic metastases who were candidates for neither resection nor radiofrequency ablation (RFA). The median number of metastases was 1 (range 1–3) and median size was 2·3 (range 0·7–6·2) cm. Toxicity was scored according to the Common Toxicity Criteria version 3.0. Local control rates were derived on tumour-based analysis.

Results
Median follow-up was 26 (range 6–57) months. Local failure was observed in nine of 31 lesions after a median interval of 22 (range 12–52) months. Actuarial 2-year local control and survival rates were 74 and 83 per cent respectively. Hepatic toxicity grade 2 or less was reported in 18 patients. Two patients had an episode of hepatic toxicity grade 3.

Conclusion
SBRT is a treatment option for patients with colorectal liver metastases who are not candidates for resection or RFA.
Gender‐based issues in interventional cardiology: A consensus statement from the Women in Innovations (WIN) Initiative
Authors: Chieffo Alaide , Hoye Angela , Mauri Fina , Mikhail Ghada W. , Ammerer Michelle , Grines Cindy , Grinfeld Liliana , Madan Mina , Presbitero Patrizia , Skelding Kimberly A. , Weiner Bonnie H. , Mehran Roxana 
Published: January 2010

AbstractCardiovascular disease (CVD) is the leading cause of mortality in women, yet studies have suggested that it is often under‐recognized. Of particular concern is the apparent suboptimal treatment of women in comparison to men, with less revascularization and use of evidence‐based medications. The Women in Innovations group of cardiologists aims to highlight these issues and change perceptions to optimize the treatment of female patients with CVD, to support future research, and to encourage and guide the training of female interventional cardiologists.© 2010 Wiley‐Liss, Inc.
Material and Energy Flow‐Based Cost Accounting
Authors: Möller A. 
Published: January 2010

AbstractThe classical approaches to cost accounting are governed by metamodels which specify the application domain of cost accounting as an instrument supporting rational decision making and controlling: Rational decision making requires relevant information about positive outcomes and related negative consequences. Even if this context will be presented in the beginning of this contribution, this article focuses on an engineering‐oriented approach to cost accounting: cost accounting as an economic evaluation of detailed engineering of technical conversions, production units, and supply chains. In such a perspective, questions regarding information demand, required modeling concepts, and integration with other instruments have to be defined and answered in another way than in conventional cost accounting.
On the implant communication and MAC protocols for WBAN
Authors: Ullah Sana , Higgins Henry , Shen Bin , Kwak Kyung Sup 
Published: January 2010

AbstractRecent advances in micro‐electro‐mechanical systems, wireless communication, low‐power intelligent sensors, and semiconductor technologies have allowed the realization of a wireless body area network (WBAN). A WBAN provides unobtrusive health monitoring for a long period of time with real‐time updates to the physician. It is widely used for ubiquitous health care, entertainment, and military applications. The implantable and wearable medical devices have several critical requirements such as power consumption, data rate, size, and low‐power medium access control (MAC) protocols. This article consists of two parts: body implant communication, which is concerned with the communication to and from a human body using radio frequency (RF) technology, and WBAN MAC protocols, which presents several low‐power MAC protocols for a WBAN with useful guidelines including a case study of IEEE 802.15.4, PB‐TDMA, and SMAC protocols. In body implant communication, the in‐body RF performance is affected considerably by the implant's depth and different polarization combinations inside the human body as well as by the muscle and fat. We observe best performance at a depth of 3 to 5 cm and not close to the human skin. Furthermore, the study of low‐power MAC protocols highlights the most important aspects of developing a novel low‐power and reliable MAC protocol for a WBAN. Copyright © 2010 John Wiley & Sons, Ltd.
Deafness and retinal degeneration in a novel USH1C knock‐in mouse model
Authors: Lentz Jennifer J. , Gordon William C. , Farris Hamilton E. , MacDonald Glen H. , Cunningham Dale E. , Robbins Carol A. , Tempel Bruce L. , Bazan Nicolas G. , Rubel Edwin W. , Oesterle Elizabeth C. , Keats Bronya J. 
Published: January 2010

AbstractUsher syndrome is the leading cause of combined deaf–blindness, but the molecular mechanisms underlying the auditory and visual impairment are poorly understood. Usher I is characterized by profound congenital hearing loss, vestibular dysfunction, and progressive retinitis pigmentosa beginning in early adolescence. Using the c.216G>A cryptic splice site mutation in Exon 3 of the USH1C gene found in Acadian Usher I patients in Louisiana, we constructed the first mouse model that develops both deafness and retinal degeneration. The same truncated mRNA transcript found in Usher 1C patients is found in the cochleae and retinas of these knock‐in mice. Absent auditory‐evoked brainstem responses indicated that the mutant mice are deaf at 1 month of age. Cochlear histology showed disorganized hair cell rows, abnormal bundles, and loss of both inner and outer hair cells in the middle turns and at the base. Retinal dysfunction as evident by an abnormal electroretinogram was seen as early as 1 month of age, with progressive loss of rod photoreceptors between 6 and 12 months of age. This knock‐in mouse reproduces the dual sensory loss of human Usher I, providing a novel resource to study the disease mechanism and the development of therapies. © 2010 Wiley Periodicals, Inc. Develop Neurobiol 70: 253–267, 2010
A review of current large‐scale mouse knockout efforts
Authors: Guan Chunmei , Ye Chao , Yang Xiaomei , Gao Jiangang 
Published: January 2010

AbstractAfter the successful completion of the human genome project (HGP), biological research in the postgenome era urgently needs an efficient approach for functional analysis of genes. Utilization of knockout mouse models has been powerful for elucidating the function of genes as well as finding new therapeutic interventions for human diseases. Gene trapping and gene targeting are two independent techniques for making knockout mice from embryonic stem (ES) cells. Gene trapping is high‐throughput, random, and sequence‐tagged while gene targeting enables the knockout of specific genes. It has been about 20 years since the first gene targeting and gene trapping mice were generated. In recent years, new tools have emerged for both gene targeting and gene trapping, and organizations have been formed to knock out genes in the mouse genome using either of the two methods. The knockout mouse project (KOMP) and the international gene trap consortium (IGTC) were initiated to create convenient resources for scientific research worldwide and knock out all the mouse genes. Organizers of KOMP regard it as important as the HGP. Gene targeting methods have changed from conventional gene targeting to high‐throughput conditional gene targeting. The combined advantages of trapping and targeting elements are improving the gene trapping spectrum and gene targeting efficiency. As a newly‐developed insertional mutation system, transposons have some advantages over retrovirus in trapping genes. Emergence of the international knockout mouse consortium (IKMP) is the beginning of a global collaboration to systematically knock out all the genes in the mouse genome for functional genomic research. genesis 48:73–85, 2010. © 2010 Wiley‐Liss, Inc.
Transgene excision in zebrafish using the phiC31 integrase
Authors: Lister James A. 
Published: January 2010

AbstractGenome engineering strategies employing site‐specific recombinases (SSRs) have become invaluable to the study of gene function in model organisms. One such SSR, the integrase encoded by the Streptomyces bacteriophage phiC31, promotes recombination between heterotypic attP and attB sites. In the present study I have examined the feasibility of the use of phiC31 integrase for intramolecular recombination strategies in zebrafish embryos. I report here that (1) phiC31 integrase is functional in zebrafish cells, (2) phiC31 integrase can excise a transgene cassette flanked by an attB and an attP site, analogous to a common use of the Cre/lox SSR system, (3) phiC31 integrase functions in the zebrafish germline, and (4) a phiC31 integrase‐estrogen receptor hormone‐binding domain variant fusion protein catalyzes attB‐attP recombination in zebrafish embryos in a 4‐hydroxytamoxifen‐dependent manner, albeit less efficiently than phiC31 alone. These features should make this a useful approach for genome manipulations in the zebrafish. genesis 48:137–143, 2010. © 2010 Wiley‐Liss, Inc.
Genetic vasectomy—Overexpression of Prm1‐EGFP fusion protein in elongating spermatids causes dominant male sterility in mice
Authors: Haueter Sabine , Kawasumi Miyuri , Asner Igor , Brykczynska Urszula , Cinelli Paolo , Moisyadi Stefan , Bürki Kurt , Peters Antoine H.F.M. , Pelczar Pawel 
Published: January 2010

AbstractTransgenic mice are vital tools in both basic and applied research. Unfortunately, the transgenesis process as well as many other assisted reproductive techniques involving embryo transfer rely on vasectomized males to induce pseudopregnancy in surrogate mothers. Vasectomy is a surgical procedure associated with moderate pain and must be carried out under full anaesthesia by qualified personnel. Eliminating the need for vasectomy would be beneficial from the economic and animal welfare point of view. Our aim was to develop a transgene‐based alternative to the surgical vasectomy procedure. We generated several transgenic mouse lines expressing a Protamine‐1 (Prm1) EGFP fusion protein under the transcriptional and translational regulatory control of Prm1. Male mice from lines showing moderate transgene expression were fully fertile whereas strong overexpression of the Prm1‐EGFP fusion protein resulted in complete and dominant male sterility without affecting the ability to mate and to produce copulatory plugs. Sterility was due to impaired spermatid maturation affecting sperm viability and motility. Furthermore, sperm having high Prm1‐EGFP levels failed to support preimplantation embryonic development following Intracytoplasmic Sperm Injection (ICSI). The “genetic vasectomy system” was further improved by genetically linking the dominant male sterility to ubiquitous EGFP expression in the soma as an easy phenotypic marker enabling rapid genotyping of transgenic males and females. This double transgenic approach represents a reliable and cost‐effective “genetic vasectomy” procedure making the conventional surgical vasectomy methodology obsolete. genesis 48:151–160, 2010. © 2010 Wiley‐Liss, Inc.
Thermal Decomposition of Ferric Oxalate Tetrahydrate in Oxidative and Inert Atmospheres: The Role of Ferrous Oxalate as an Intermediate
Authors: Hermankova Pavla , Hermanek Martin , Zboril Radek 
Published: January 2010

AbstractThe thermal decomposition of ferric oxalate tetrahydrate Fe2(C2O4)3·4H2O was studied in dynamic oxidative and inert atmospheres by using a simultaneous thermogravimetric (TG) and differential scanning calorimetric (DSC) analytical device equipped with an evolved gas analyzer (EGA). Solid‐state decomposition products formed during the decomposition were analyzed by 57Fe Mössbauer spectroscopy, in situ and ex situ X‐ray powder diffraction, and magnetic measurements. In the dynamic inert atmosphere, we observed the formation of a tiny amount of superparamagnetic iron oxide (most likely Fe3O4) together with a majority of ferrous oxalate (FeC2O4) and remains of undecomposed Fe2(C2O4)3 after the first decomposition step, which finished at 210 °C. The astonishing presence of the oxidic phase at such low temperatures is a highly probable side effect of the main reduction action of the electrons on the FeIII cations in the ferric oxalate structure, thus resulting in the creation of intermediate FeC2O4. The final product of decomposition of the FeC2O4 intermediate in a dynamic inert atmosphere is a mixture of wüstite (FexO), α‐iron (α‐Fe), and magnetite (Fe3O4). Their proportion accurately reflects actual disproportionation/synproportionation/redisproportionation processes likely encouraged by the preserved size and morphology of the initial ferric oxalate crystals and that are dependent on temperature. In the oxidative atmosphere, the decomposition proceeds in the three overlapped stages that include dehydration, the astonishing reductive formation of FeC2O4 as an intermediate, and final decarboxylation to hematite (α‐Fe2O3). The principal effect of the experimental conditions on the amount of intermediate formation of FeC2O4 in the oxidative atmosphere is also discussed and evaluated from the isothermal experiments carried out at 180 °C.
Synthesis and Characterization of Diiron Thiadithiolate Complexes Related to the Active Site of [FeFe]‐Hydrogenases
Authors: Song Li‐Cheng , Li Qing‐Shan , Yang Zhi‐Yong , Hua Yu‐Juan , Bian Hong‐Zhu , Hu Qing‐Mei 
Published: January 2010

AbstractOn the basis of preparation of the known complex [Fe2(μ‐SCH2)2S(CO)6] (A) by a new method involving condensation of [(μ‐LiS)2Fe2(CO)6] with excess S(CH2Br)2, twelve new diiron thiadithiolates as mimics of the [FeFe]‐hydrogenase active site have been synthesized by substitution of the CO ligand and coordination at the central S atom of complex A with appropriate reagents. Treatment of A with 1 equiv. of the monodentate ligands PPh3 and [(η5‐C5H5)(η5‐Ph2PC5H4)Fe] in the presence of Me3NO and with 1 equiv. tBuNC and cyclohexyl isocyanide gave the single [2Fe3S]‐cluster‐containing monosubstituted complexes [Fe2(μ‐SCH2)2S(CO)5(L1)] (1, L1 = PPh3; 2, L1 = (η5‐C5H5)(η5‐Ph2PC5H4)Fe; 3, L1 = tBuNC; 4, L1 = C6H11NC), whereas the double [2Fe3S]‐cluster‐containing disubstituted complexes [{Fe2(μ‐SCH2)2S(CO)5}2(L2)] [5, L2 = 4,4′‐(Ph2P)2(C6H4)2; 6, L2 = trans‐Ph2PCH=CHPPh2; 7, L2 = 1,4‐(CN)2C6H4; 8, L2 = (η5‐Ph2PC5H4)2Fe; 9, L2 = (η5‐Ph2PC5H4)2Ru] were produced by reaction of A with 0.5 equiv. of the corresponding bidentate ligands in the presence of Me3NO. In addition, the single [2Fe3S]‐cluster‐containing complexes in which the central S atom is coordinated, [{Fe2(μ‐SCH2)2S(CO)6}{(η5‐MeC5H4)(CO)2Fe}(BF4)] (10), [{Fe2(μ‐SCH2)2S(CO)6}{Cr(CO)5}] (11), and [{Fe2(μ‐SCH2)2S(CO)6}{W(CO)5}] (12), could be obtained by reaction of complex A with the in situ prepared [{(η5‐MeC5H4)(CO)2Fe}(BF4)], [Cr(CO)5(thf)], and [W(CO)5(thf)], respectively. While complex 3 was found to be able to reduce the proton of the weak acid Et3NHCl to give H2, the X‐ray crystallographic study confirmed that (i) each P atom of the phosphane ligands in 1 and 8 occupies an apical position at the Fe atoms, (ii) the isocyanide ligand in 3 lies in a basal position of the Fe atom, and (iii) the (η5‐MeC5H4)(CO)2Fe, Cr(CO)5, and W(CO)5 units in 10–12 are linked to the central S atom of complex A by an equatorial bond from the two fused six‐membered rings of their [2Fe3S]‐cluster cores.
Tailoring visual images to fit: Value creation in persuasive messages
Authors: Mannetti Lucia , Giacomantonio Mauro , Higgins E. Tory , Pierro Antonio , Kruglanski Arie W. 
Published: January 2010

AbstractThe present studies aimed to extend Regulatory Fit Theory in the domain of persuasive communication by (a) using printed advertisement images without any verbal claim, instead of purely or mostly verbal messages; (b) selecting the images to fit the distinct orientations of regulatory mode rather than regulatory focus; and (c) priming regulatory mode orientation instead of relying on chronic prevalence of either locomotion or assessment orientation. We found that recipients primed with a locomotion orientation experienced fit, and were more persuaded, when exposed to “dynamic” versus “static” visual images; conversely, recipients primed with an assessment orientation experienced fit and were more persuaded when exposed to “static” versus “dynamic” images. Our findings show that the experience of fit can be induced by visual messages, resulting in positive effects in terms of attitude toward product advertisement and estimated price of advertised products. Copyright © 2010 John Wiley & Sons, Ltd.
Pt–Fe and Pt–Ni Carbon Nitride‐Based ‘Core–Shell’ ORR Electrocatalysts for Polymer Electrolyte Membrane Fuel Cells
Authors: Di Noto V. , Negro E. 
Published: January 2010

AbstractThis report describes the preparation of new bimetal carbon nitride (CN)‐based ‘core–shell’ electrocatalysts for the oxygen reduction reaction (ORR) for application in polymer electrolyte membrane fuel cells. The ‘core’ consists of graphitic carbon nanoparticles and the ‘shell’ of CN electrocatalysts. These latter are based on Pt–Fe and Pt–Ni active sites which, with respect to pure Pt, are devised for their improved ORR performance. This morphology allows to stabilise the active sites of the electrocatalysts by a chemical coordination process with the support as the coordinating agent. The wt% of N is lower than 2% to obtain electrocatalytic ‘shells’ with a good electron conductivity. The electrocatalysts with a ‘core–shell’ morphology were extensively characterised from the chemical, thermogravimetric and morphological points of view. Electrochemical measurements carried out by cyclic voltammetry using a thin‐film rotating ring‐disk electrode (CV–TF‐RRDE) method allowed to study the specific surface area of the active sites and to determine the ORR performance and the reaction mechanism. Finally, the materials were mounted at the cathode of single fuel cells and their performance was tested in operative conditions. The Pt mass required to achieve 1 kW was as low as 0.3 g, versus 1.11 g of the reference membrane‐electrode assembly.
Aberrant methylation impairs low density lipoprotein receptor‐related protein 1B tumor suppressor function in gastric cancer
Authors: Lu Yen‐Jung , Wu Chi‐Sheng , Li Hsin‐Pai , Liu Hao‐Ping , Lu Chang‐Yi , Leu Yu‐Wei , Wang Chia‐Siu , Chen Lih‐Chyang , Lin Kwang‐Huei , Chang Yu‐Sun 
Published: January 2010

AbstractDNA methylation plays a significant role in tumor progression. In this study, we used CpG microarray and differential methylation hybridization approaches to identify low density lipoprotein receptor‐related protein 1B (LRP1B) as a novel epigenetic target in gastric cancer. LRP1B was hypermethylated in four gastric cancer cell lines, and low LRP1B mRNA expression was associated with high methylation levels in gastric cancer cell lines. Addition of a DNA methylation inhibitor (5‐Aza‐dC) restored the mRNA expression of LRP1B in these cell lines, indicating that DNA methylation is involved in regulating LRP1B expression. In 45 out of 74 (61%) clinical samples, LRP1B was highly methylated; LRP1B mRNA expression was significantly lower in 15 out of 19 (79%, P < 0.001) gastric tumor tissues than in corresponding adjacent normal tissues. In addition, ectopic expression of mLRP1B4 in gastric cancer cell lines suppressed cell growth, colony formation and tumor formation in nude mice. These results collectively indicate that LRP1B is a functional tumor suppressor gene in gastric cancer and that is regulated by DNA methylation. © 2010 Wiley‐Liss,Inc.
Effect of brain‐derived neurotrophic factor haploinsufficiency on stress‐induced remodeling of hippocampal neurons
Authors: Magariños A.M. , Li C.J. , Gal Toth J. , Bath K.G. , Jing D. , Lee F.S. , McEwen B.S. 
Published: January 2010

AbstractChronic restraint stress (CRS) induces the remodeling (i.e., retraction and simplification) of the apical dendrites of hippocampal CA3 pyramidal neurons in rats, suggesting that intrahippocampal connectivity can be affected by a prolonged stressful challenge. Since the structural maintenance of neuronal dendritic arborizations and synaptic connectivity requires neurotrophic support, we investigated the potential role of brain derived neurotrophic factor (BDNF), a neurotrophin enriched in the hippocampus and released from neurons in an activity‐dependent manner, as a mediator of the stress‐induced dendritic remodeling. The analysis of Golgi‐impregnated hippocampal sections revealed that wild type (WT) C57BL/6 male mice showed a similar CA3 apical dendritic remodeling in response to three weeks of CRS to that previously described for rats. Haploinsufficient BDNF mice (BDNF±) did not show such remodeling, but, even without CRS, they presented shorter and simplified CA3 apical dendritic arbors, like those observed in stressed WT mice. Furthermore, unstressed BDNF± mice showed a significant decrease in total hippocampal volume. The dendritic arborization of CA1 pyramidal neurons was not affected by CRS or genotype. However, only in WT mice, CRS induced changes in the density of dendritic spine shape subtypes in both CA1 and CA3 apical dendrites. These results suggest a complex role of BDNF in maintaining the dendritic and spine morphology of hippocampal neurons and the associated volume of the hippocampal formation. The inability of CRS to modify the dendritic structure of CA3 pyramidal neurons in BDNF± mice suggests an indirect, perhaps permissive, role of BDNF in mediating hippocampal dendritic remodeling. © 2010 Wiley‐Liss, Inc.
X‐ray reconstruction of moving morphology (XROMM): precision, accuracy and applications in comparative biomechanics research
Authors: Brainerd Elizabeth L. , Baier David B. , Gatesy Stephen M. , Hedrick Tyson L. , Metzger Keith A. , Gilbert Susannah L. , Crisco Joseph J. 
Published: January 2010

AbstractX‐Ray Reconstruction of Moving Morphology (XROMM) comprises a set of 3D X‐ray motion analysis techniques that merge motion data from in vivo X‐ray videos with skeletal morphology data from bone scans into precise and accurate animations of 3D bones moving in 3D space. XROMM methods include: (1) manual alignment (registration) of bone models to video sequences, i.e., Scientific Rotoscoping; (2) computer vision‐based autoregistration of bone models to biplanar X‐ray videos; and (3) marker‐based registration of bone models to biplanar X‐ray videos. Here, we describe a novel set of X‐ray hardware, software, and workflows for marker‐based XROMM. Refurbished C‐arm fluoroscopes retrofitted with high‐speed video cameras offer a relatively inexpensive X‐ray hardware solution for comparative biomechanics research. Precision for our biplanar C‐arm hardware and analysis software, measured as the standard deviation of pairwise distances between 1 mm tantalum markers embedded in rigid objects, was found to be ±0.046 mm under optimal conditions and ±0.084 mm under actual in vivo recording conditions. Mean error in measurement of a known distance between two beads was within the 0.01 mm fabrication tolerance of the test object, and mean absolute error was 0.037 mm. Animating 3D bone models from sets of marker positions (XROMM animation) makes it possible to study skeletal kinematics in the context of detailed bone morphology. The biplanar fluoroscopy hardware and computational methods described here should make XROMM an accessible and useful addition to the available technologies for studying the form, function, and evolution of vertebrate animals. J. Exp. Zool. 313A:262–279, 2010. © 2010 Wiley‐Liss, Inc.