bluuebunny/crossref_metadata_2025_split huggingface dataset:
Volume electron microscopy in injured rat brain validates white matter microstructure metrics from diffusion MRI
Authors: Coronado-Leija Ricardo , Abdollahzadeh Ali , Lee Hong-Hsi , Coelho Santiago , Ades-Aron Benjamin , Liao Ying , Salo Raimo A. , Tohka Jussi , Sierra Alejandra , Novikov Dmitry S. , Fieremans Els 
Published: June 2024

AbstractBiophysical modeling of diffusion MRI (dMRI) offers the exciting potential of bridging the gap between the macroscopic MRI resolution and microscopic cellular features, effectively turning the MRI scanner into a noninvasive in vivo microscope. In brain white matter, the Standard Model (SM) interprets the dMRI signal in terms of axon dispersion, intra- and extra-axonal water fractions, and diffusivities. However, for SM to be fully applicable and correctly interpreted, it needs to be carefully evaluated using histology. Here, we perform a comprehensive histological validation of the SM parameters, by characterizing white matter (WM) microstructure in sham and injured rat brains using volume electron microscopy and ex vivo dMRI. Sensitivity is evaluated by how well each SM metric correlates with its histological counterpart, and specificity by the lack of correlation with other, non-corresponding histological features. Compared to previously developed SM estimators with constraints, our results show that SMI is the most sensitive and specific. Furthermore, we derive the functional form of the fiber orientation distribution based on its exponentially decreasing rotational invariants. This comprehensive comparison with histology may facilitate the clinical adoption of in vivo dMRI-derived SM parameters as biomarkers for neurological disorders.
Tensions and Trade-Offs – Staff’s Understanding of Children as Rights Holders in Secure Care
Authors: Sallnäs Marie , Enell Sofia , Mattsson Titti 
Published: June 2024

Abstract
This article explores how staff in Swedish secure care value and understand the rights of the children and young people in their care. To be a staff member in secure care means having a professional role that includes viewing and relating to young people as individual rights holders in a setting where care and treatment shall be provided to a group of young people. However, this occurs in an environment characterised by strong coercive and controlling elements. The study shows that the viewing and handling of children’s rights is dependent on various trade-offs that staff make. Negotiations about what should be seen as rights frequently take place, leading to tensions regarding children’s status as individual rights holders. The study adds knowledge about how staff describe the nature of these tensions in the daily life of secure care units. Implications for practice are discussed.
The Mission of Social Sciences and Missions
Authors: Sharkey Heather J. 
Published: June 2024

Abstract
This article assesses the “mission” of the journal Social Sciences and Missions as it approaches its twentieth anniversary, starting from its origins as a forum for interdisciplinary study of colonial-era Christian missions, especially in Africa. It surveys major debates that the journal has followed regarding gender, migration, and more. It reflects on its revised manifesto of 2021, committing to the study of missions as neither a “theological category” nor exclusively Christian phenomenon “but rather [as] a type of social action” and mode of “religious intervention in social space.” Building on this history, this article argues, the journal should critically question the nature of religion and the “religious;” cover non-Christian topics more fully; and encompass organizations that may not be recognizably “faith based.” Broader comparative focus will sharpen the journal’s focus on missions as movements that have aimed to channel and promote social change, often with far-reaching and ambiguous consequences.
Whom to Cater to?
Authors: Goddeeris Idesbald , Athreya Aditi 
Published: June 2024

Abstract
After 1947, the Jesuit province of Ranchi faced several challenges: the context of a newly independent country, increasing modernization and industrialization, and growing societal diversity resulting from immigration and urbanization. The Jesuits not only needed to upgrade their primary education, but also had to meet a rising demand for more middle and high schools. They had to make choices: Should they keep uplifting marginalized communities? Or should they cater to the middle class and elite? This article, predominantly based on internal documents of the Society of Jesus in Ranchi, demonstrates that the Jesuits did not make clear decisions and invested their energy in various types of schools and students. As a result, problems and internal discussions persisted.
Safe Soundscapes
Authors: Giorgi Alberta 
Published: June 2024

Abstract
This article explores religious-feminist podcasting. Religious feminists have found a place in digital spaces, including podcasts, to connect and thrive, making themselves visible and heard to their religious communities and the broader public. Drawing on interviews with Christian feminist podcasters located in Europe and North America, this paper advances two contributions. First, unlike other forms of digital media, podcasts do not favor the emergence and circulation of specific forms of feminism. Second, focusing on materiality and the role of intimacy and emotions embedded in podcasting, I argue that Christian feminist podcasting can be seen as a form of audibility activism and that through the creation of ‘safe soundscapes’ in which to experience alternative feeling rules, Christian feminist podcasters contribute to broadening conversations concerning feminism and religion.
Christian Activism in the Hong Kong Pro-Democracy Movement: A History from 1984 to 2019
Authors: Lai Tsz-him 
Published: June 2024

Abstract
Churches in Hong Kong have served as contractors to the state since its British colonization. To secure their political and social privileges, these churches have often been fashioned into apologists for the state. This mentality was not widely challenged until the 1980s. Since the 1984 Sino-British Joint Declaration, some Hong Kong church leaders, theologians, and laypeople have started to articulate a democratic vision for civil society. This paper employs the notion of “religious activism” as an analytical framework to examine the role of Hong Kong Christianity in the pro-democracy movement. The influential voices and actions of Christians in 1984, 1989, 2003, 2014, and 2019 are chronologically summarized. Each political event triggered divergent reflections on the political participation of Christians. This paper offers an analysis of genealogical narratives of Christian activism and offers a prediction on the future of this political participation, particularly under the imposition of the National Security Law.
24. What Are Couples Made of?
Authors: Rotkirch Anna 
Published: June 2024

Compared to the evolutionary psychology of mating, the evolutionary demography of unions is little developed. We know quite a bit about why and how people have sex, much less about why and how they have spouses. Yet couples continue to be a central building block of families, the biosocial tie within which most adults live, most sex takes place, and through which most children are made and raised. Arguably, sexual selection in humans happens through long-term pair bonds rather than short-term relationships.
Evolutionary theory approaches unions as reproductive contracts: a precarious balance of conflict and compromise between individual reproductive and sexual strategies. Sexual strategies are predicted to vary especially with age, gender and resources, but also with ecological and social conditions such as increasing gender equality and lower fertility.
This chapter discusses the formation of unions in high-income, increasingly gender equal societies from the intersection of family demography and evolutionary studies. How is selection of spouses affected by having more highly educated women in the population? Why does contemporary family formation often involve a stage of cohabitation before marriage? I argue that sexual strategies theory could move beyond the division into short-term versus long-term pair bonds, and suggest that cohabitation represents one mid-term form of temporal and psychological commitment to a romantic partner.
Ground-Based Remote Sensing of Aerosol, Clouds, Dynamics, and Precipitation in Antarctica: First Results from the 1-Year COALA Campaign at Neumayer Station III in 2023
Authors: Radenz Martin , Engelmann Ronny , Henning Silvia , Schmithüsen Holger , Baars Holger , Frey Markus M. , Weller Rolf , Bühl Johannes , Jimenez Cristofer , Roschke Johanna , Muser Lukas Ole , Wullenweber Nellie , Zeppenfeld Sebastian , Griesche Hannes , Wandinger Ulla , Seifert Patric 
Published: June 2024

Abstract
Novel observations of aerosol and clouds by means of ground-based remote sensing have been performed in Antarctica over the Ekström Ice Shelf on the coast of Dronning Maud Land at Neumayer Station III (70.67°S, 8.27°W) from January to December 2023. The deployment of the OCEANET-Atmosphere remote sensing observatory in the framework of the Continuous Observations of Aerosol-Cloud Interaction (COALA) campaign has brought Aerosol, Clouds and Trace Gases Research Infrastructure (ACTRIS) aerosol and cloud profiling capabilities next to meteorological and air chemistry in situ observations at the Antarctic station. We present an overview of the site, the instrumental setup, and data analysis strategy and introduce 3 scientific highlights from austral fall and winter, namely, 1) observations of a persistent mixed-phase cloud embedded in a plume of marine aerosol. Remote sensing–based retrievals of cloud-relevant aerosol properties and cloud microphysical parameters confirm that the free-tropospheric mixed-phase cloud layer formed in an aerosol-limited environment. 2) Two extraordinary warm air intrusions: one with intense snowfall produced the equivalent of 10% of the yearly snow accumulation and a second one with record-breaking maximum temperatures and heavy icing due to supercooled drizzle. 3) Omnipresent aerosol layers in the stratosphere. Our profiling capabilities could show that 50% of the 500-nm aerosol optical depth of 0.06 was caused by stratospheric aerosol, while the troposphere was usually pristine. As demonstrated by these highlights, the 1-yr COALA observations will serve as a reference dataset for the vertical structure of aerosol and clouds above the region, enabling future observational and modeling studies to advance understanding of atmospheric processes in Antarctica.
Impact of the Summer Atmospheric Heat Source over the Tibetan Plateau on Interannual Variability of Meridional Circulation on the North Side of the Tibetan Plateau
Authors: Luo Hongyu , Yu Haipeng , Hu Zeyong , Zhou Jie , Zhang Bofei , Yang Yaoxian , Cheng Shanling , Gong Yongqi , Ren Yu 
Published: June 2024

Abstract
The summer atmospheric heat source (AHS) over the Tibetan Plateau (TP) induces meridional circulations in TP and its surrounding areas. Previous studies mainly focused on the monsoon circulation on the south side of TP, while the formation and maintenance mechanisms of meridional circulation on its north side remain unclear. This study compared three calculation methods of the AHS, analyzed the spatial–temporal variability of the summer AHS over the TP, and discussed its influence on the interannual variability of meridional circulation on the north side of the TP based on the two-dimensional decomposition method of atmospheric circulation and sensitivity experiments. The results indicate that in the positive AHS anomalies years, the diabatic heating of condensation latent release in southeastern TP could motivate anomalous ascending motion. Simultaneously, the increased meridional temperature gradient between the mid- and high latitudes of East Asia leads to an enhanced southward westerly jet. In this context, the region on the north side of TP, located on the north side of the westerly jet entrance, is affected by negative anomalous relative vorticity advection, prevailing anomalous descending motion, which makes the descending branch of meridional circulation significantly presented. Unlike previous studies that considered the descending branch of meridional circulation as the compensation for upward flow, the results of the linear baroclinic model (LBM) verify that the descending branch is mainly influenced by the vorticity advection related to regional scale variability of the westerly jet. This study reveals the physical mechanism of meridional circulation on the north side of TP, which offers valuable implications for seasonal forecasting in TP and Northwest China.
Comparison of Tumor Non-specific and PD-L1 Specific Imaging by Near-Infrared Fluorescence/Cerenkov Luminescence Dual-Modality In-situ Imaging
Authors: Zhang Linhan , Zhao Lianmeng , Lin Xue , Zhao Sheng , Pan Wenbin , Wang Dandan , Sun Zhongqi , Li Jinping , Liang Zonghui , Zhang Rongjun , Jiang Huijie 
Published: June 2024

Background Labeled antibodies are excellent imaging agents in oncology to non-invasively visualize cancer-related antigens expression levels. However, tumor tracer uptake (TTU) of specific antibodies in-vivo may be inferior to non-specific IgG in some cases. Objectives To explore factors affecting labeled antibody visualization by PD-L1 specific and non-specific imaging of nude mouse tumors. Methods TTU was observed in RKO model on Cerenkov luminescence (CL) and near-infrared fluorescence (NIRF) imaging of radionuclide 131I or NIRF dyes labeled Atezolizumab and IgG. A mixture of NIRF dyes labeled Atezolizumab and 131I-labeled IgG was injected, and TTU was observed in the RKO and HCT8 model by NIRF/CL dual-modality in-situ imaging. TTU were observed by 131I-labeled Atezolizumab and IgG in-vitro distribution. Results Labeled IgG concentrated more in tumors than Atezolizumab. NIRF/CL imaging in 24 to 168 h showed that TTU gradually decreased over time, which decreased more slowly on CL imaging compared to NIRF imaging. The distribution data in-vitro showed that TTU of 131I-labeled IgG was higher than that of 131I-labeled Atezolizumab at any time point. Conclusion Non-specific IgG may not be suitable as a control for Atezolizumab in comparing tumor PD-L1 expression in nude mice via labeled antibody optical imaging under certain circumstances.
Early-life infection depletes preleukemic cells in a mouse model of hyperdiploid B-cell acute lymphoblastic leukemia
Authors: Farrokhi Ali , Atre Tanmaya , Salitra Samuel , Aletaha Maryam , Márquez Ana Citlali , Gynn Matthew , Fidanza Mario , Jo Sumin , Rolf Nina , Simmons Karen , Duque-Afonso Jesus , Cleary Michael L. , Seif Alix E. , Kollmann Tobias , Gantt Soren , Reid Gregor S. D. 
Published: June 2024

Abstract
Epidemiological studies report opposing influences of infection on childhood B-cell acute lymphoblastic leukemia (B-ALL). Although infections in the first year of life appear to exert the largest impact on leukemia risk, the effect of early pathogen exposure on the fetal preleukemia cells (PLC) that lead to B-ALL has yet to be reported. Using cytomegalovirus (CMV) infection as a model early-life infection, we show that virus exposure within 1 week of birth induces profound depletion of transplanted E2A-PBX1 and hyperdiploid B-ALL cells in wild-type recipients and in situ–generated PLC in Eμ-ret mice. The age-dependent depletion of PLC results from an elevated STAT4-mediated cytokine response in neonates, with high levels of interleukin (IL)-12p40–driven interferon (IFN)-γ production inducing PLC death. Similar PLC depletion can be achieved in adult mice by impairing viral clearance. These findings provide mechanistic support for potential inhibitory effects of early-life infection on B-ALL progression and could inform novel therapeutic or preventive strategies.
Genome-wide identification and integrated analysis of the FAR1/FHY3 gene family and genes expression analysis under methyl jasmonate treatment in Panax ginseng C. A. Mey.
Authors: Jiang Yang , Zeng Zixia , He Gaohui , Liu Mengna , Liu Chang , Liu Mingming , Lv Tingting , Wang Aimin , Wang Yi , Zhao Mingzhu , Wang Kangyu , Zhang Meiping 
Published: June 2024

AbstractGinseng (Panax ginseng C. A. Mey.) is an important and valuable medicinal plant species used in traditional Chinese medicine, and its metabolite ginsenoside is the primary active ingredient. The FAR1/FHY3 gene family members play critical roles in plant growth and development as well as participate in a variety of physiological processes, including plant development and signaling of hormones. Studies have indicated that methyl jasmonate treatment of ginseng adventitious roots resulted in a significant increase in the content of protopanaxadiol ginsenosides. Therefore, it is highly significant to screen the FAR1/FHY3 gene family members in ginseng and preliminarily investigate their expression patterns in response to methyl jasmonic acid signaling. In this study, we screened and identified the FAR1/FHY3 family genes in the ginseng transcriptome databases. And then, we analyzed their gene structure and phylogeny, chromosomal localization and expression patterns, and promoter cis-acting elements, and made GO functional annotations on the members of this family. After that, we treated the ginseng adventitious roots with 200 mM methyl jasmonate and investigated the trend of the expression of four genes containing the largest number of methyl jasmonate cis-acting elements at different treatment times. All four genes were able to respond to methyl jasmonate, the most significant change was in the PgFAR40 gene. This study provides data support for subsequent studies of this family member in ginseng and provides experimental reference for subsequent validation of the function of this family member under methyl jasmonic acid signaling.
Revealing the Arabidopsis AtGRP7 mRNA binding proteome by specific enhanced RNA interactome capture
Authors: Reichel Marlene , Schmidt Olga , Rettel Mandy , Stein Frank , Köster Tino , Butter Falk , Staiger Dorothee 
Published: June 2024

Abstract
Background
The interaction of proteins with RNA in the cell is crucial to orchestrate all steps of RNA processing. RNA interactome capture (RIC) techniques have been implemented to catalogue RNA- binding proteins in the cell. In RIC, RNA-protein complexes are stabilized by UV crosslinking in vivo. Polyadenylated RNAs and associated proteins are pulled down from cell lysates using oligo(dT) beads and the RNA-binding proteome is identified by quantitative mass spectrometry. However, insights into the RNA-binding proteome of a single RNA that would yield mechanistic information on how RNA expression patterns are orchestrated, are scarce.

Results
Here, we explored RIC in Arabidopsis to identify proteins interacting with a single mRNA, using the circadian clock-regulated Arabidopsis thaliana GLYCINE-RICH RNA-BINDING PROTEIN 7 (AtGRP7) transcript, one of the most abundant transcripts in Arabidopsis, as a showcase. Seedlings were treated with UV light to covalently crosslink RNA and proteins. The AtGRP7 transcript was captured from cell lysates with antisense oligonucleotides directed against the 5’untranslated region (UTR). The efficiency of RNA capture was greatly improved by using locked nucleic acid (LNA)/DNA oligonucleotides, as done in the enhanced RIC protocol. Furthermore, performing a tandem capture with two rounds of pulldown with the 5’UTR oligonucleotide increased the yield. In total, we identified 356 proteins enriched relative to a pulldown from atgrp7 mutant plants. These were benchmarked against proteins pulled down from nuclear lysates by AtGRP7 in vitro transcripts immobilized on beads. Among the proteins validated by in vitro interaction we found the family of Acetylation Lowers Binding Affinity (ALBA) proteins. Interaction of ALBA4 with the AtGRP7 RNA was independently validated via individual-nucleotide resolution crosslinking and immunoprecipitation (iCLIP). The expression of the AtGRP7 transcript in an alba loss-of-function mutant was slightly changed compared to wild-type, demonstrating the functional relevance of the interaction.

Conclusion
We adapted specific RNA interactome capture with LNA/DNA oligonucleotides for use in plants using AtGRP7 as a showcase. We anticipate that with further optimization and up scaling the protocol should be applicable for less abundant transcripts.
Effects of selenium enrichment on fermentation characteristics, selenium content and microbial community of alfalfa silage
Authors: Sun Pengbo , Ge Gentu , Sun Lin , Du Shuai , Liu Yichao , Yan Xingquan , Zhang Jiawei , Zhang Yuhan , Wang Zhijun , Jia Yushan 
Published: June 2024

Abstract
Background
Selenium is essential for livestock and human health. The traditional way of adding selenium to livestock diets has limitations, and there is a growing trend to provide livestock with a safe and efficient source of selenium through selenium-enriched pasture. Therefore, this study was conducted to investigate the effects of selenium enrichment on fermentation characteristics, selenium content, selenium morphology, microbial community and in vitro digestion of silage alfalfa by using unenriched (CK) and selenium-enriched (Se) alfalfa as raw material for silage.

Results
In this study, selenium enrichment significantly increased crude protein, soluble carbohydrate, total selenium, and organic selenium contents of alfalfa silage fresh and post-silage samples, and it significantly decreased neutral detergent fiber and acid detergent fiber contents (p < 0.05). Selenium enrichment altered the form of selenium in plants, mainly in the form of SeMet and SeMeCys, which were significantly higher than that of CK (p < 0.05). Selenium enrichment could significantly increase the lactic acid content, reduce the pH value, change the diversity of bacterial community, promote the growth of beneficial bacteria such as Lactiplantibacillus and inhibit the growth of harmful bacteria such as Pantoea, so as to improve the fermentation quality of silage. The in vitro digestibility of dry matter (IVDMD), in vitro digestibility of acid detergent fibers (IVADFD) and in vitro digestibility of acid detergent fibers (IVNDFD) of silage after selenium enrichment were significantly higher than those of CK (p < 0.05).

Conclusion
This study showed that the presence of selenium could regulate the structure of the alfalfa silage bacterial community and improve alfalfa silage fermentation quality. Selenium enrichment measures can change the morphology of selenium in alfalfa silage products, thus promoting the conversion of organic selenium.
The vaginal microbial signatures of preterm birth woman
Authors: Li Huan , Han Mengzhen , Xu Junnan , Li Na , Cui Hong 
Published: June 2024

AbstractTo explore the differences of vaginal microbes in women with preterm birth (PTB), and to construct prediction model. We searched for articles related to vaginal microbiology in preterm women and obtained four 16S rRNA-sequence datasets. We analyzed that for species diversity and differences, and constructed a random forest model with 20 differential genera. We introduce an independent whole genome-sequencing (WGS) data for validation. In addition, we collected vaginal and cervical swabs from 33 pregnant women who delivered spontaneously full-term and preterm infants, performed WGS in our lab to further validate the model. Compared to term birth (TB) samples, PTB women vagina were characterized by a decrease in Firmicutes, Lactobacillus, and an increase in diversity accompanied by the colonization of pathogenic bacteria such as Gardnerella, Atopobium and Prevotella. Twenty genus markers, including Lactobacillus, Prevotella, Streptococcus, and Gardnerella performed well in predicting PTB, with study-to-study transfer validation and LODO validation, different gestation validation showing good results, and in two independent cohorts (external WGS cohorts and woman samples WGS cohorts) in which the accuracy was maintained. PTB women have unique vaginal microbiota characteristics. A predictive model of PTB was constructed and its value validated from multiple perspectives.