bluuebunny/crossref_metadata_2025_split huggingface dataset:
Uses and Functions of Formulaic Sequences in Second Language Speech: An Exploration of the Foundations of Fluency
Authors: Wood David 
Published: June 2010

Abstract:Formulaic sequences are fixed combinations of words that have a range of functions and uses in speech production and communication, and seem to be cognitively stored and retrieved by speakers as if they were single words. They can facilitate fluency in speech by making pauses shorter and less frequent, and allowing longer runs of speech between pauses. The present study was undertaken to identify the uses and functions of formulaic sequences in the development of speech fluency in narrative retelling in English as a second language (ESL). Spontaneous spoken narrative retells by ESL learners were analyzed for ways in which use of formulaic sequences may have facilitated fluency growth over a six-month period, be they pragmatic, functional, or strategic. Five categories of formula use emerged: repetition of a formula; use of multiple formulas to extend a run; reliance on one formula; use of self-talk and filler formulas; and use of formulas as rhetorical devices. These categories are illustrated by excerpts from transcripts of learner speech.
Comparing Focus on Form and Focus on FormS in Second-Language Vocabulary Learning
Authors: Laufer Batia 
Published: June 2010

Abstract:The study compares the effectiveness of Focus on Form (FonF) and Focus on FormS (FonFs) approaches in learning new L2 words by 158 high-school learners of English as L2. In phase 1 of the study, the FonF group read a text containing the target words, discussed it in small groups, and answered comprehension questions. The FonFs group studied the target words as discrete items with their meanings and examples of usage. In phase 2 of the study, all learners received the target words with their meanings and studied them for 15 minutes for a quiz. They were tested immediately after each phase, and two weeks later.Results revealed significantly higher scores for FonFs group after phase 1, but the difference disappeared after phase 2. As learners cannot be expected to study all vocabulary for tests, form-focused instruction - and particularly FonFs - is claimed to be indispensable for L2 vocabulary learning.
‘Like Pulling Teeth’: Oral Discourse Practices in a Culturally Diverse Language Arts Classroom
Authors: Wiltse Lynne 
Published: June 2010

Abstract:This article examines community practices, in particular oral discourse practices, in a junior high school classroom in an urban area of Western Canada. The purpose of the research was to determine what contribution a ‘community of practice’ (COP) framework could make to the study of social interaction in a classroom setting. Qualitative research methods were used to explore student-student and student-teacher interaction in a culturally and linguistically diverse Grade 9 classroom. Of particular interest was why a language arts teacher who claimed to love teaching through dialogue rarely initiated classroom discussion. Study findings demonstrated that the teacher's practices were shaped by the social structure of the classroom community. The article explores the teacher's role in disrupting cultural practices that limit students' participation. In conclusion, the article makes an argument for scaffolding to be added to the COP framework if it is to be utilized to advantage in classroom settings and suggests incorporating hybrid literacy practices in multilingual contexts.
Apprentissage coopératif et prises de parole en langue cible dans deux classes d'immersion
Authors: Mattar Cathérine , Blondin Christiane 
Published: June 2010

Abstract: Providing students with enough opportunities to speak and communicate in the target language is one of the challenges faced by both linguistic immersion and conventional language courses. The study documented here shows that cooperative learning, the efficiency of which has been demonstrated in various contexts, could help educators meet this challenge. Cooperative learning activities suggested by one of the researchers were organized by the teachers of two Grade 2 classes (7- and 8-year-old students) in partial immersion (German in one case, Dutch in the other). The speech acts among two heterogeneous groups of four children per class were recorded and analysed. The article shows that every pupil spoke not only in their L1 but also in the target language and that speech acts were fairly equally distributed within each group.
Elementary School EFL Learners' Vocabulary Learning: The Effects of Post-Reading Activities
Authors: Atay Derin , Kurt Gökce 
Published: June 2010

Abstract:As language learning involves the acquisition of thousands of words, teachers and learners alike would like to know how vocabulary learning can be fostered, especially in EFL settings where learners frequently acquire impoverished lexicons, despite years of formal study. Research indicates that reading is important but not sufficient for second-language vocabulary learning, and that it should be supplemented by post-reading activities to enhance students' vocabulary knowledge. The present study investigates the effects of two types of post-reading activities: discrete written tasks on their own and a combination of written tasks along with interactive tasks on the vocabulary acquisition of young learners in an EFL setting. A total of 62 Grade 6 students in two classes in a public school in Turkey participated in the study. Data were collected by the Cambridge English Test (CYLET) and Vocabulary Knowledge Scale (VKS). Results showed that the experimental group students outperformed the control group students in acquisition of both selected and unselected vocabulary items. The use of interactive tasks as post-reading activities proved to be an effective way of enhancing the L2 vocabulary knowledge of young learners.
Effect of temperature and strain rate on strain hardening and deformation mechanisms of high manganese austenitic steels
Authors: Bäumer Annette , Jiménez José Antonio , Bleck Wolfgang 
Published: June 2010

Abstract
The effect of temperature and strain rate on the mechanical properties of three Fe – Mn austenitic steels with Mn contents of 20 mass.% or above has been studied by tensile tests performed at strain rates ranging from 3 × 10−3 to 200s−1 and at temperatures between −160 and +140 °C. In these steels mechanical properties and strain hardening behaviour result from the competition between different deformation mechanisms including homogeneous or inhomogeneous dislocation glide, strain induced twinning or martensite formation. Thus, the microstructure evolution during deformation was investigated. For two of the steels, a relation of the deformation mechanisms (twinning and dislocation glide) at room temperature with the grain size was found. On the other hand, for the third steel at temperatures lower than 60 °C martensite is progressively formed during deformation. For the three steels, a decreasing volume fraction of twins or martensite with increasing testing temperature was found leading consequently to a decrease in flow stress and strain hardening.
Long-term outcome of lumbar disc surgery: an experience from Pakistan
Authors: Bakhsh Ahmed 
Published: June 2010

Object
The author conducted a study to determine the long-term outcome of lumbar disc surgery on relief of sciatic leg pain.

Methods
This was a retrospective observational study conducted at Fauji Foundation Hospital, Rawalpindi, Pakistan. The author reviewed medical records of 68 patients who underwent lumbar disc surgery for sciatic pain during the period 1995–2004. All patients were physically examined and interviewed.

Results
Lumbar disc surgery yielded complete pain relief in 79.41% of the cases. In 14.7% of the cases surgery failed to give any pain relief, and in 5.88% it yielded partial pain relief. At up to 10 years postoperatively, 27.77% of patients remained absolutely pain free. Pain recurred in 12.82% of cases after 1 year, in 35.89% during the first 5 years, and in 51.28% after 10 years. Pain recurred in the same leg in 63.88%, in the contralateral leg in 19.44%, and in both legs in 16.66%. Neurological deficits did not improve in any case except in 1 case of foot drop. New neurological deficits developed postoperatively in 8.82% of cases in the form of foot drop and calf muscle weakness.

Conclusions
Surgery provided immediate pain relief in 79.41% of cases, but the long-term outcome of lumbar disc surgery was not satisfactory.
Results and risk factors for recurrence following single-level tubular lumbar microdiscectomy
Authors: Moliterno Jennifer A. , Knopman Jared , Parikh Karishma , Cohan Jessica N. , Huang Q. Daisy , Aaker Grant D. , Grivoyannis Anastasia D. , Patel Ashwin R. , Härtl Roger , Boockvar John A. 
Published: June 2010

Object
The use of minimally invasive surgical techniques, including microscope-assisted tubular lumbar microdiscectomy (tLMD), has gained increasing popularity in treating lumbar disc herniations (LDHs). This particular procedure has been shown to be both cost-efficient and effective, resulting in outcomes comparable to those of open surgical procedures. Lumbar disc herniation recurrence necessitating reoperation, however, remains an issue following spinal surgery, with an overall reported incidence of approximately 3–13%. The authors' aim in the present study was to report their experience using tLMD for single-level LDH, hoping to provide further insight into the rate of surgical recurrence and to identify potential risk factors leading to this complication.

Methods
The authors retrospectively reviewed the cases of 217 patients who underwent tLMD for single-level LDH performed identically by 2 surgeons (J.B., R.H.) between 2004 and 2008. Evaluation for LDH recurrence included detailed medical chart review and telephone interview. Recurrent LDH was defined as the return of preoperative signs and symptoms after an interval of postoperative resolution, in conjunction with radiographic demonstration of ipsilateral disc herniation at the same level and pathological confirmation of disc material. A cohort of patients without recurrence was used for comparison to identify possible risk factors for recurrent LDH.

Results
Of the 147 patients for whom the authors were able to definitively assess symptomatic recurrence status, 14 patients (9.5%) experienced LDH recurrence following single-level tLMD. The most common level involved was L5–S1 (42.9%) and the mean length of time to recurrence was 12 weeks (range 1.5–52 weeks). Sixty-four percent of the patients were male. In a comparison with patients without recurrence, the authors found that relatively lower body mass index was significantly associated with recurrence (p = 0.005), such that LDH in nonobese patients was more likely to recur.

Conclusions
Recurrence rates following tLMD for LDH compare favorably with those in patients who have undergone open discectomy, lending further support for its effectiveness in treating single-level LDH. Nonobese patients with a relatively lower body mass index, in particular, appear to be at greater risk for recurrence.
Delineating Grassland Bird Conservation Areas in the U.S. Prairie Pothole Region
Authors: Johnson Rex R. , Granfors Diane A. , Niemuth Neal D. , Estey Michael E. , Reynolds Ronald E. 
Published: June 2010

Abstract
Conservation of birds is increasingly focused on the importance of landscape characteristics to sustain populations. Implementing conservation on a landscape scale requires reliable spatial models that provide biological context for conservation actions. Before species-specific models relating grassland birds to their habitat at landscape scales existed, we created a conceptual model and applied it to spatial data to identify priority grassland habitats for the protection and restoration of populations of area sensitive grassland birds in the Prairie Pothole Region. Since that time, these Grassland Bird Conservation Areas have been widely used to guide conservation, and variations of these models have been adopted in other regions; however, the process used to delineate them (i.e., the conceptual models) is poorly understood by many users. We describe that process here and offer perspectives on the utility and limitations of conceptual models, especially on the value of making assumptions that commonly underlie management decisions explicitly, thereby making the assumptions testable, and hopefully increasing management transparency, credibility, and efficiency.
Umbilical Cord Blood T Cells Respond against the Melan-A/MART-1 Tumor Antigen and Exhibit Reduced Alloreactivity as Compared with Adult Blood-Derived T Cells
Authors: Merindol Natacha , Grenier Anne-Julie , Caty Martine , Charrier Emily , Duval Arnaud , Duval Michel , Champagne Martin A. , Soudeyns Hugo 
Published: June 2010

Abstract
Umbilical cord blood (UCB) is increasingly used as a source of hematopoietic progenitor cells to treat a variety of disorders. UCB transplant is associated with comparatively reduced incidence of graft-versus-host disease, robust graft versus leukemia effect, and relatively high incidence of opportunistic infections, three processes in which donor-derived T lymphocytes are known to be predominantly involved. To examine the differential functionality of UCB T cells, CD8+ T cells specific for the melanoma-associated HLA-A2–restricted Melan-A26–35 A27L peptide were isolated from HLA-A2+ and HLA-A2− UCB samples and HLA-A2+ and HLA-A2− adult peripheral blood using A2/Melan-A tetramers. In UCB samples, A2/Melan-A+ CD8+ T cells were detected at a frequency of 0.04%, were more frequent in HLA-A2+ UCB, and were polyclonal and mostly naive. Consistent with Ag-driven expansion, the frequency of A2/Melan-A+ CD8+ T cells was increased following stimulation with cognate peptide or polyclonal activation, they acquired cell-surface markers reflective of effector/memory differentiation, their TCR repertoire became oligoclonal, and they expressed cytolytic activity and produced IFN-γ. Although functional properties of A2/Melan-A+ CD8+ T cells derived from HLA-A2+ UCB resembled those of HLA-A2+ adult peripheral blood, they were more likely to reach terminal differentiation following polyclonal stimulation and produced less IFN-γ in response to cognate peptide. A2/Melan-A+ CD8+ T cells from HLA-A2− UCB were poorly cytolytic, produced little IFN-γ, and were predominantly monofunctional or nonfunctional. These properties of UCB-derived CD8+ T cells could contribute to the reduced incidence of graft-versus-host disease and heightened incidence of opportunistic infections observed following UCB transplant.
The Relative Timing of Exposure to Phagocytosable Particulates and to Osteoclastogenic Cytokines Is Critically Important in the Determination of Myeloid Cell Fate
Authors: James Douglas E. , Nestor Bryan J. , Sculco Thomas P. , Ivashkiv Lionel B. , Ross F. Patrick , Goldring Steven R. , Purdue P. Edward 
Published: June 2010

Abstract
During granulomatous inflammatory reactions, myeloid cells can differentiate into activated phagocytic macrophages, wound-healing macrophages, foreign body giant cells, and bone-resorbing osteoclasts. Although it is appreciated that a variety of stimuli, including cytokines, cell–matrix interactions, and challenge with foreign materials can influence myeloid cell fate, little is known of how these signals integrate during this process. In this study, we have investigated the cross talk between receptor activator of NF-κB ligand (RANKL)-induced osteoclastogenesis and particle phagocytosis-induced activation of human monocytes. Understanding interconnected signals is of particular importance to disorders, such as periprosthetic osteolysis, in which granulomatous inflammation is initiated by particle phagocytosis in proximity to bone and leads to inflammatory bone loss. Using cell-based osteoclastogenesis and phagocytosis assays together with expression analysis of key regulators of osteoclastogenesis, we show in this study that phagocytosis of disease-relevant particles inhibits RANKL-mediated osteoclastogenesis of human monocytes. Mechanistically, phagocytosis mediates this effect by downregulation of RANK and c-Fms, the receptors for the essential osteoclastogenic cytokines RANKL and M-CSF. RANKL pretreatment of monocytes generates preosteoclasts that are resistant to RANK downregulation and committed to osteoclast formation, even though they retain phagocytic activity. Thus, the relative timing of exposure to phagocytosable particulates and to osteoclastogenic cytokines is critically important in the determination of myeloid cell fate.
Enhancement of Antiviral Immunity by Small Molecule Antagonist of Suppressor of Cytokine Signaling
Authors: Ahmed Chulbul M. I. , Dabelic Rea , Martin James P. , Jager Lindsey D. , Haider S. Mohammad , Johnson Howard M. 
Published: June 2010

Abstract
Suppressors of cytokine signaling (SOCSs) are negative regulators of both innate and adaptive immunity via inhibition of signaling by cytokines such as type I and type II IFNs. We have developed a small peptide antagonist of SOCS-1 that corresponds to the activation loop of JAK2. SOCS-1 inhibits both type I and type II IFN activities by binding to the kinase activation loop via the kinase inhibitory region of the SOCS. The antagonist, pJAK2(1001–1013), inhibited the replication of vaccinia virus and encephalomyocarditis virus in cell culture, suggesting that it possesses broad antiviral activity. In addition, pJAK2(1001–1013) protected mice against lethal vaccinia and encephalomyocarditis virus infection. pJAK2(1001–1013) increased the intracellular level of the constitutive IFN-β, which may play a role in the antagonist antiviral effect at the cellular level. Ab neutralization suggests that constitutive IFN-β may act intracellularly, consistent with recent findings on IFN-γ intracellular signaling. pJAK2(1001–1013) also synergizes with IFNs as per IFN-γ mimetic to exert a multiplicative antiviral effect at the level of transcription, the cell, and protection of mice against lethal viral infection. pJAK2(1001–1013) binds to the kinase inhibitory region of both SOCS-1 and SOCS-3 and blocks their inhibitory effects on the IFN-γ activation site promoter. In addition to a direct antiviral effect and synergism with IFN, the SOCS antagonist also exhibits adjuvant effects on humoral and cellular immunity as well as an enhancement of polyinosinic-polycytidylic acid activation of TLR3. The SOCS antagonist thus presents a novel and effective approach to enhancement of host defense against viruses.
Mechanism and Localization of CD8 Regulatory T Cells in a Heart Transplant Model of Tolerance
Authors: Li Xian Liang , Ménoret Séverine , Bezie Séverine , Caron Lise , Chabannes Dominique , Hill Marcelo , Halary Franck , Angin Mathieu , Heslan Michèle , Usal Claire , Liang Liang , Guillonneau Carole , Le Mauff Brigitte , Cuturi Maria Cristina , Josien Régis , Anegon Ignacio 
Published: June 2010

Abstract
Despite accumulating evidence for the importance of allospecific CD8+ regulatory T cells (Tregs) in tolerant rodents and free immunosuppression transplant recipients, mechanisms underlying CD8+ Treg-mediated tolerance remain unclear. By using a model of transplantation tolerance mediated by CD8+ Tregs following CD40Ig treatment in rats, in this study, we show that the accumulation of tolerogenic CD8+ Tregs and plasmacytoid dendritic cells (pDCs) in allograft and spleen but not lymph nodes was associated with tolerance induction in vascularized allograft recipients. pDCs preferentially induced tolerogenic CD8+ Tregs to suppress CD4+ effector cells responses to first-donor Ags in vitro. When tolerogenic CD8+ Tregs were not in contact with CD4+ effector cells, suppression was mediated by IDO. Contact with CD4+ effector cells resulted in alternative suppressive mechanisms implicating IFN-γ and fibroleukin-2. In vivo, both IDO and IFN-γ were involved in tolerance induction, suggesting that contact with CD4+ effector cells is crucial to modulate CD8+ Tregs function in vivo. In conclusion, CD8+ Tregs and pDCs interactions were necessary for suppression of CD4+ T cells and involved different mechanisms modulated by the presence of cell contact between CD8+ Tregs, pDCs, and CD4+ effector cells.
Low-Level Exposure to HIV Induces Virus-Specific T Cell Responses and Immune Activation in Exposed HIV-Seronegative Individuals
Authors: Restrepo Clara , Rallón Norma I. , del Romero Jorge , Rodríguez Carmen , Hernando Victoria , López Mariola , Peris Alejandra , Lozano Sara , Sempere-Ortells José M. , Soriano Vincent , Benito José M. 
Published: June 2010

Abstract
HIV-specific T cells response and T cell activation are frequently seen in exposed seronegative individuals (ESN). In this study, we report HIV-specific response and level of T cell activation in ESN partners of HIV-infected patients presenting low or undetectable levels of HIV-RNA. We evaluated 24 HIV-serodiscordant couples. ESN were classified into three categories of exposure to HIV (very low, low, and moderate-high), considering levels of HIV-RNA in their infected partner and frequency of sexual high-risk practices within the last 12 mo. HIV-specific T cell responses and activation levels in T cell subsets were evaluated by flow cytometry. We reported that 54% of ESN had detectable HIV-specific T cells response, being the highest prevalence seen in the low exposure group (64%). Several T cell subsets were significantly increased in ESN when compared with controls: CD4+CD38+ (p = 0.006), CD4+HLA-DR−CD38+ (p = 0.02), CD4+CD45RA+CD27+HLA-DR−CD38+ (p = 0.002), CD8+CD45RA+CD27+CD38−HLA-DR+ (p = 0.02), and CD8+CD45RA+CD27−CD38+HLA-DR+ (p = 0.03). Activation of CD8+ T cells was increased in ESN with detectable HIV T cell responses compared with ESN lacking these responses (p = 0.04). Taken together, these results suggest that persistent but low sexual HIV exposure is able to induce virus-specific T cells response and immune activation in a high proportion of ESN, suggesting that virus exposure may occur even in conditions of maximal viral suppression in the HIV-infected partner.
The Exocytosis of Lytic Granules Is Impaired in Vti1b- or Vamp8-Deficient CTL Leading to a Reduced Cytotoxic Activity following Antigen-Specific Activation
Authors: Dressel Ralf , Elsner Leslie , Novota Peter , Kanwar Namita , Fischer von Mollard Gabriele 
Published: June 2010

Abstract
The exocytosis of cytotoxic proteins stored in lytic granules of activated CTL is a key event during killing of target cells. Membrane fusion events that are mediated by soluble N-ethylmaleimide-sensitive-factor attachment protein receptor (SNARE) proteins are crucial, as demonstrated by patients with familial hemophagocytic lymphohistocytosis type 4 who have mutations in the SNARE protein syntaxin-11 that result in an impaired degranulation of cytotoxic cells. We found an increased mRNA expression of the SNARE protein genes Vti1b and Vamp8 during Ag-specific activation of CTL from TCR-transgenic OT-I mice. Therefore, we investigated the cytolytic activity of CTL from TCR-transgenic Vti1b and Vamp8 knockout mice. At 3 d as well as at 4 d of Ag-specific stimulation, the degranulation of CTL was significantly reduced in Vti1b and Vamp8 knockout mice, as determined by cell surface expression of the degranulation marker CD107a. After 3 d of Ag-specific stimulation, the cytolytic activity of Vti1b- and Vamp8-deficient CTL was reduced to ≈50% compared with heterozygous controls. However, 4 d after stimulation, the cytotoxic activity of Vti1b- as well as Vamp8-deficient CTL was not impaired anymore. The capacity of Vti1b- and Vamp8-deficient dendritic cells to process Ags and to stimulate the proliferation of CTL was not reduced, arguing against an indirect effect on the activation of CTL. These findings suggest a role of the SNARE proteins vti1b and vesicle-associated membrane protein 8 in the degranulation of CTL. However, a deficiency can apparently be compensated and affects only transiently the cytotoxic activity of CTL during their development to armed effector cells.