bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of F/G-actin ratio and actin turn-over rate on NADPH oxidase activity in microglia
Authors: Rasmussen Izabela , Pedersen Line H , Byg Luise , Suzuki Kazuhiro , Sumimoto Hideki , Vilhardt Frederik 
Published: September 2010

Abstract

Background
Most in vivo studies that have addressed the role of actin dynamics in NADPH oxidase function in phagocytes have used toxins to modulate the polymerization state of actin and mostly effects on actin has been evaluated by end point measurements of filamentous actin, which says little about actin dynamics, and without consideration for the subcellular distribution of the perturbed actin cytoskeleton.

Results
Here, we in addition to toxins use conditional expression of the major actin regulatory protein LIM kinase-1 (LIMK1), and shRNA knock-down of cofilin to modulate the cellular F/G-actin ratio in the Ra2 microglia cell line, and we use Fluorescence Recovery after Photobleaching (FRAP) in β-actin-YFP-transduced cells to obtain a dynamic measure of actin recovery rates (actin turn-over rates) in different F/G-actin states of the actin cytoskeleton. Our data demonstrate that stimulated NADPH oxidase function was severely impaired only at extreme actin recovery rates and F/G-actin ratios, and surprisingly, that any moderate changes of these parameters of the actin cytoskeleton invariably resulted in an increased NADPH oxidase activity.

Conclusion
moderate actin polymerization and depolymerization both increase the FMLP and PMA-stimulated NADPH oxidase activity of microglia, which is directly correlated with neither actin recovery rate nor F/G- actin ratio. Our results indicate that NADPH oxidase functions in an enhanced state of activity in stimulated phagocytes despite widely different states of the actin cytoskeleton.
Prevention of post-surgical abdominal adhesions by a novel biodegradable thermosensitive PECE hydrogel.
Authors: Yang Bing , Gong ChangYang , Qian ZhiYong , Zhao Xia , Li ZhengYu , Qi XiaoRong , Zhou ShengTao , Zhong Qian , Luo Feng , Wei YuQuan 
Published: September 2010

Abstract

Background
Post-operative peritoneal adhesions are common and serious complications for modern medicine. We aim to prevent post-surgical adhesions using biodegradable and thermosensitive poly(ethylene glycol)-poly(ε-caprolactone)-poly(ethylene glycol) (PEG-PCL-PEG, PECE) hydrogel. In this work, we investigated the effect of PECE hydrogel on preventing post-surgical abdominal adhesions in mouse and rat models.

Results
The PECE hydrogel in sol state could be transformed into gel in less than 20 s at 37°C. In addition, the PECE hydrogel could be easily adhered to the damaged peritoneal surfaces, and be gradually degraded and absorbed by the body within 14 days along with the healing of peritoneal wounds. A notable efficacy of the PECE hydrogel in preventing peritoneal adhesions was demonstrated in the animal models. In contrast, all untreated animals developed adhesions requiring sharp dissection. Furthermore, no significant histopathological changes were observed in main organs of the hydrogel-treated animals.

Conclusion
Our results suggested that the thermosensitive PECE hydrogel was an effective, safe, and convenient agent on preventing post-surgical intro-abdominal adhesions.
A Pleiotrophin C-terminus peptide induces anti-cancer effects through RPTPβ/ζ
Authors: Diamantopoulou Zoi , Bermek Oya , Polykratis Apostolos , Hamma-Kourbali Yamina , Delbé Jean , Courty José , Katsoris Panagiotis 
Published: September 2010

Abstract

Background
Pleiotrophin, also known as HARP (Heparin Affin Regulatory Peptide) is a growth factor expressed in various tissues and cell lines. Pleiotrophin participates in multiple biological actions including the induction of cellular proliferation, migration and angiogenesis, and is involved in carcinogenesis. Recently, we identified and characterized several pleiotrophin proteolytic fragments with biological activities similar or opposite to that of pleiotrophin. Here, we investigated the biological actions of P(122-131), a synthetic peptide corresponding to the carboxy terminal region of this growth factor.

Results
Our results show that P(122-131) inhibits in vitro adhesion, anchorage-independent proliferation, and migration of DU145 and LNCaP cells, which express pleiotrophin and its receptor RPTPβ/ζ. In addition, P(122-131) inhibits angiogenesis in vivo, as determined by the chicken embryo CAM assay. Investigation of the transduction mechanisms revealed that P(122-131) reduces the phosphorylation levels of Src, Pten, Fak, and Erk1/2. Finally, P(122-131) not only interacts with RPTPβ/ζ, but also interferes with other pleiotrophin receptors, as demonstrated by selective knockdown of pleiotrophin or RPTPβ/ζ expression with the RNAi technology.

Conclusions
In conclusion, our results demonstrate that P(122-131) inhibits biological activities that are related to the induction of a transformed phenotype in PCa cells, by interacing with RPTPβ/ζ and interfering with other pleiotrophin receptors. Cumulatively, these results indicate that P(122-131) may be a potential anticancer agent, and they warrant further study of this peptide.
Prognostic significance and therapeutic implications of centromere protein F expression in human nasopharyngeal carcinoma
Authors: Cao Jing-Yan , Liu Li , Chen Shu-Peng , Zhang Xing , Mi Yan-Jun , Liu Zhi-Gang , Li Man-Zhi , Zhang Hua , Qian Chao-Nan , Shao Jian-Yong , Fu Li-Wu , Xia Yun-Fei , Zeng Mu-Sheng 
Published: September 2010

AbstractBackgroundOur recent cDNA microarray data showed that centromere protein F (CENP-F) is significantly upregulated in primary cultured nasopharyngeal carcinoma (NPC) tumor cells compared with normal nasopharyngeal epithelial cells. The goal of this study was to further investigate the levels of CENP-F expression in NPC cell lines and tissues to clarify the clinical significance of CENP-F expression in NPC as well as the potential therapeutic implications of CENP-F expression.MethodsReal-time RT-PCR and western blotting were used to examine CENP-F expression levels in normal primary nasopharyngeal epithelial cells (NPEC), immortalized nasopharyngeal epithelial cells and NPC cell lines. Levels of CENP-F mRNA were determined by real-time RT-PCR in 23 freshly frozen nasopharyngeal biopsy tissues, and CENP-F protein levels were detected by immunohistochemistry in paraffin sections of 202 archival NPC tissues. Statistical analyses were applied to test for prognostic associations. The cytotoxicities of CENP-F potential target chemicals, zoledronic acid (ZOL) and FTI-277 alone, or in combination with cisplatin, in NPC cells were determined by the MTT assay.ResultsThe levels of CENP-F mRNA and protein were higher in NPC cell lines than in normal and immortalized NPECs. CENP-F mRNA level was upregulated in nasopharyngeal carcinoma biopsy tissues compared with noncancerous tissues. By immunohistochemical analysis, CENP-F was highly expressed in 98 (48.5%) of 202 NPC tissues. Statistical analysis showed that high expression of CENP-F was positively correlated with T classification (P< 0.001), clinical stage (P< 0.001), skull-base invasion (P< 0.001) and distant metastasis (P= 0.012) inversely correlated with the overall survival time in NPC patients. Multivariate analysis showed that CENP-F expression was an independent prognostic indicator for the survival of the patient. Moreover, we found that ZOL or FTI-277 could significantly enhance the chemotherapeutic sensitivity of NPC cell lines (HONE1 and 6-10B) with high CENP-F expression to cisplatin, although ZOL or FTI-277 alone only exhibited a minor inhibitory effect to NPC cells.ConclusionOur data suggest that CENP-F protein is a valuable marker of NPC progression, and CENP-F expression is associated with poor overall survival of patients. In addition, our data indicate a potential benefit of combining ZOL or FTI-277 with cisplatin in NPC suggesting that CENP-F expression may have therapeutic implications.
Cytostatic and anti-angiogenic effects of temsirolimus in refractory mantle cell lymphoma
Authors: Wang Li , Shi Wen-Yu , Wu Zhi-Yuan , Varna Mariana , Wang Ai-Hua , Zhou Li , Chen Li , Shen Zhi-Xiang , Lu He , Zhao Wei-Li , Janin Anne 
Published: September 2010

Abstract
Mantle cell lymphoma (MCL) is a rare and aggressive type of B-cell non-Hodgkin's lymphoma. Patients become progressively refractory to conventional chemotherapy, and their prognosis is poor. However, a 38% remission rate has been recently reported in refractory MCL treated with temsirolimus, a mTOR inhibitor.
Here we had the opportunity to study a case of refractory MCL who had tumor regression two months after temsirolimus treatment, and a progression-free survival of 10 months. In this case, lymph node biopsies were performed before and six months after temsirolimus therapy. Comparison of the two biopsies showed that temsirolimus inhibited tumor cell proliferation through cell cycle arrest, but did not induce any change in the number of apoptotic tumor cells. Apart from this cytostatic effect, temsirolimus had an antiangiogenic effect with decrease of tumor microvessel density and of VEGF expression. Moreover, numerous patchy, well-limited fibrotic areas, compatible with post-necrotic tissue repair, were found after 6-month temsirolimus therapy. Thus, temsirolimus reduced tumor burden through associated cytostatic and anti-angiogenic effects.
This dual effect of temsirolimus on tumor tissue could contribute to its recently reported efficiency in refractory MCL resistant to conventional chemotherapy.
Functional Consequences of Dual Oxidase-Thyroperoxidase Interaction at the Plasma Membrane
Authors: Fortunato Rodrigo Soares , Lima de Souza Elaine Cristina , Hassani Rabii Ameziane-el , Boufraqech Myriem , Weyemi Urbain , Talbot Monique , Lagente-Chevallier Odile , de Carvalho Denise Pires , Bidart Jean-Michel , Schlumberger Martin , Dupuy Corinne 
Published: September 2010

Context: Thyroperoxidase (TPO) and dual oxidase (DUOX) are present at the apical membrane of thyrocytes, where TPO catalyzes thyroid hormone biosynthesis in the presence of H2O2 produced by DUOX. Both enzymes are colocalized and associated, but the consequences of this interaction remain obscure.Objective: The objective of this study was to evaluate the functional consequences of TPO-DUOX interaction at the plasma membrane.Design: The functional consequences of DUOX-TPO interaction were studied by measuring extracellular H2O2 concentration and TPO activity in a heterologous system. For this purpose, HEK293 cells were transiently transfected with a combination of human TPO with human DUOX1 or DUOX2 in the presence of their respective maturation factors, DUOXA1 or DUOXA2. The effect of human DUOX2 mutants in which cysteine residues in the N-terminal domain were replaced by glycines was also analyzed.Results: We observed that production of H2O2 decreases both TPO and DUOX activities. We show that TPO presents a catalase-like effect that protects DUOX from inhibition by H2O2. This catalase-like effect depends on the association between both enzymes, which probably occurs through the DUOX peroxidase-like domain because this effect was not observed with human DUOX2 mutants.Conclusion: The DUOX-TPO association at the plasma membrane is relevant for normal enzyme properties. Normally, TPO consumes H2O2 produced by DUOX, decreasing the availability of this substance at the apical membrane of thyrocytes and, in turn, probably decreasing the oxidative damage of macromolecules.
Robustly Optimal Wind Turbine for Swirl Expansion and Decay
Authors: Farthing S P 
Published: September 2010

The wind turbine optima of propeller actuator disc theories are problematic. The large optimal windmill interference doubles the wake area from the vortex cylinder used to justify the propeller blade element momentum (BEM) theory. The general momentum (GM) theory incorporates expansion, but its optimum windrotor power blows up below a tip speed ratio of about unity where the BEM and experimental optima are low. Even without expansion, the GM and vortex theory optimum can still affect infinitely more gross power flux than in undisturbed wind. The inner massive swirl of their optimal wake is unstable or unrealizable by many indicators. Rather than a cylindrical wake, the BEM is better founded on negligible suction from low swirl in a predominately axial wake expanded after instability and bursting of the hub vorticity outwards to cancel the axial component of the tip vorticity. If moderate swirl at outer high speed ratios can expand first with pressure regain, the optimum local power refines up to 5 per cent higher. Then only as this expanded outer axial vorticity is finally cancelled to relieve the suction from the swirl does the inner BEM flow slow and expand. The inner and outer axial velocities are equal with no radial velocity at local speed ratio 0.57 in the optimal rotor. Direct experimental verification of this dissipated expanded swirl head (DESH) refinement of the BEM is proposed further to support from measurements and computations in the literature. The design blade chord and pitch that make the DESH optimum robust to small changes in windspeed are little changed from the time-proven BEM design, whereas the GM robust chord has a singularity.
Security-Aspekte bei der Entwicklung eingebetteter Software
Authors: Adamczyk Heiko 
Published: September 2010

Zusammenfassung
Anforderungen an die Softwareentwicklung von funktional sicheren Geräten (Safety) werden in einschlägigen Normen bereits klar definiert. Dagegen gibt es bislang kaum vergleichbare Normen zur Abdeckung der IT-Sicherheit, die konkrete Anforderungen an die Software für das industrielle Umfeld liefert. Vor dem Anwendungshintergrund der industriellen Automatisierung wird dazu in diesem Beitrag das Konzept eines Frameworks vorgestellt, mit dessen Hilfe sicherheitskritische eingebettete Geräte unter Beachtung von IT-Security-Aspekten entwickeln werden können.
A renewal theoretic result in portfolio theory under transaction costs with multiple risky assets
Authors: Irle Albrecht , Prelle Claas 
Published: September 2010

Abstract
We consider a portfolio optimization problem in a Black-Scholes model with n stocks, in which an investor faces both fixed and proportional transaction costs. The performance of an investment strategy is measured by the average return of the corresponding portfolio over an infinite time horizon. At first, we derive a representation of the portfolio value process which only depends on the relative fractions of the total portfolio value that the investor holds in the different stocks. This representation allows us to consider these so-called risky fractions as the decision variables of the investor. We show a certain kind of stationarity (Harris recurrence) for a quite flexible class of strategies (constant boundary strategies). Then, using renewal theoretic methods, we are able to describe the asymptotic return by the behaviour of the risky fractions in a “typical” period between two trades. Our results generalize those of [irl1], who considered a financial market model with one bond and one stock, to a market with a finite number n>1 of stocks.
On the mean residual waiting time of records
Authors: Bdair Omar M. , Raqab Mohammad Z. 
Published: September 2010

Abstract
In this paper, we examine the mean residual waiting time of record values from a sequence of identically independent random variables with a common continuous distribution F. Under the condition that the (m+1)-st shock has not arrived by time t>0, we obtain a simplified expression for the mean residual waiting time of the (n+1)-st shock. We investigate some monotonicity and aging properties for the mean residual waiting time of records. Further, it is shown that the underlying distribution function F can be recovered via the functional relationships between the mean residual waiting times of records.
Laser-Doppler-Distanzsensor mit Phasenauswertung zur Messung von Position und Form rotierender Objekte
Authors: Günther Philipp , Pfister Thorsten , Büttner Lars , Czarske Jürgen 
Published: September 2010

Zusammenfassung
In diesem Beitrag wird ein neuartiger optischer Sensor vorgestellt, der die gleichzeitige Messung von axialer Position und tangentialer Geschwindigkeit einer sich bewegenden rauen Festkörperoberfläche ermöglicht. Dafür wird ein erweitertes Laser-Doppler-Velozimeter genutzt, bei dem zwei gegeneinander verkippte Interferenzstreifensysteme überlagert werden. Es wird eine Positionsauflösung von 280 nm an rauen Festkörperoberflächen erreicht. Im Gegensatz zu herkömmlichen Messtechniken, wie der Triangulation, ist diese Positionsauflösung unabhängig von der Geschwindigkeit des Messobjektes. Dadurch ermöglicht dieser Sensor die präzise Positions- und Formbestimmung von schnell drehenden Objekten.
Energieautarke Sensorik im Flugzeug
Authors: Bartholomé Kilian , Jägle Martin , Ebling Dirk G. , König Jan , Jacqot Alexandre , Böttner Harald 
Published: September 2010

Zusammenfassung
Der Einsatz energieautarker Sensorik bietet die Möglichkeit, wartungsfrei Sensoren über einen sehr langen Zeitraum an unzugänglichen Orten zu betreiben. Durch den Einsatz thermoelektrischer Generatoren wird die Verwendung energieautarker Systeme überall da ermöglicht, wo Temperaturgradienten mit wenigen Kelvin existieren. In diesem Artikel wird der Einsatz eines durch Thermogeneratoren versorgten energieautarken Systems für den Einsatz im Flugzeug vorgestellt und die Minimierung thermischer Widerstände als wesentlicher Punkt für eine maximale Leistungsausbeute diskutiert.
Terahertz-Wellen — ein neuer Spektralbereich für die industrielle Messtechnik
Authors: Ellrich Frank , Weinland Tristan , Klier Jens , Jonuscheit Joachim , Beigang René 
Published: September 2010

Zusammenfassung
Zwischen Radar und Infrarot liegen die so genannten Terahertz-Wellen mit Wellenlängen im Bereich zwischen 30 μm und 3 mm. Diese können mit Hilfe moderner optischer Methoden effizient erzeugt und empfindlich nachgewiesen werden. Die technische Nutzung der Strahlung eröffnet neue Möglichkeiten sowohl in der Grundlagenforschung als auch speziell in der industriellen Messtechnik. Die Entwicklung eines fasergekoppelten Terahertz-Messsystems ermöglicht den Einsatz in unterschiedlichen industriellen Anwendungen, die an ausgewählten Beispielen vorgestellt werden.
Reproductive Patterns among Danish Women with Oral Clefts
Authors: Yttri Janne Elin , Christensen Kaare , Knudsen Lisbeth B. , Bille Camilla 
Published: September 2010

Objective The aim of this study was to compare reproduction patterns among Danish women born with isolated oral clefts versus the Danish background population. Design and Setting A nationwide population-based historic cohort study based on three registers: The Danish Facial Cleft Register, The Danish Civil Registration System, and the Fertility of Women and Couples Dataset. Participants Through linkages of the registers, the number of children and the exact age at childbirth of all Danish women born with an oral cleft from 1950 through 1988 (N = 1931) were obtained. These data were compared with similar data for the entire Danish female background population (N = 1,184,390). Results and Conclusions This study suggests that childlessness is higher among women born with an oral cleft (55%) than among women in the Danish background population (44%). Excluding women without children, women born with oral cleft had an average of 1.98 children per woman. This did not differ significantly from the background population (2.02 children/woman). Furthermore, the present study found that Danish women born with oral clefts on average were older (27.3 years of age) than the background population (24.7 years of age) when they had their first child (difference = 2.6 years, confidence interval = 2.41 to 2.80). Conclusion Danish women born with an oral cleft more often are childless or have their first child later, but if they have children, they tend to have as many children as other Danish women. Social, psychological, and biological reasons might be responsible for the reduced fertility.
Lower Thoracic Spine Pain in a 33-Year-Old Female
Authors: Robertson Eric K. , Evans Amanda L. 
Published: September 2010

The patient was a 33-year-old woman with a chief complaint of midthoracic spine pain. Anterior-posterior and lateral radiographs of the thoracic spine revealed superior and inferior endplate deformities and a mild reduction of stature of the T9 vertebral body, which were suspicious for a fracture. Computed tomography scans revealed cortical irregularity of the vertebral body of T9, however, magnetic resonance imaging of the thoracic spine was negative. Given the magnetic resonance imaging findings, the smooth, well-defined cortical defect, and the absence of paraspinal hemorrhaging on the computed tomography scan, it was hypothesized that this may not have been an acute fracture. Rather, the cortical irregularity of the anterior vertebral body of T9 seen on computed tomography could have instead represented normal nutrient vascular channels. The patient was referred to an orthopaedic surgeon, who recommended conservative management by a physical therapist. J Orthop Sports Phys Ther 2010;40(8):533. doi:10.2519/jospt.2010.0412
Juvenile Osteochondritis Dissecans of the Knee
Authors: Swain James H. , Grimm Nathan L. , Shea Kevin G. 
Published: September 2010

The patient was a 13-year-old male soccer player who was referred to a physical therapist with complaints of persistent worsening left medial knee pain with weight-bearing activity. Physical examination revealed no signs of joint effusion, lower extremity range-of-motion or strength discrepancies, ligamentous compromise, or tenderness to palpation. However, the patient reported discomfort with weight bearing, and a slightly antalgic gait was observed. Knee radiographs revealed a concave lucency involving the medial femoral condyle, consistent with an osteochondritis dissecans lesion. Magnetic resonance imaging confirmed an osteochondritis dissecans lesion of the medial femoral condyle, and the patient was diagnosed with stage I juvenile osteochondritis dissecans of the knee. Conservative management by a physical therapist was the recommended treatment, and after 6 months, the patient no longer complained of left knee pain, and repeat knee radiographs demonstrated evidence of a healing osteochondritis dissecans lesion. J Orthop Sports Phys Ther 2010;40(8):534. doi:10.2519/jospt.2010.0413