bluuebunny/crossref_metadata_2025_split huggingface dataset:
AAV plasmid DNA simplifies liver‐directed in vivo gene therapy: comparison of expression levels after plasmid DNA‐, adeno‐associated virus‐ and adenovirus‐mediated liver transfection
Authors: Doenecke Axel , Krömer Alexander , Scherer Marcus N. , Schlitt Hans‐Jürgen , Geissler Edward K. 
Published: August 2010

AbstractBackgroundSuccessful liver gene therapy depends on efficient gene transfer techniques and long‐lasting gene expression after successful transfer. Over the last decades, important progress has been made with the introduction of viral vectors using animal models, although their use is hampered by a complex and costly preparation compared to the simple and cost‐effective preparation of plasmid DNA. These problems become even more critical when considering the application of viral vectors in human gene therapy and gene therapy trials. In a previous study, we were able to show that the hydrodynamics‐based gene transfer of plasmid‐DNA, containing the adeno‐associated‐virus specific inverted terminal repeats (AAV‐ITR), prolongs gene expression in the liver, although it remained unclear whether plasmid gene transfer could achieve similar expression levels compared to viral‐vector gene transfer.MethodsRat livers were transfected in‐vivo with AAV‐ITR‐containing plasmid‐DNA using a modified hydrodynamics‐based procedure. Expression levels were monitored thereafter and compared with expression levels after viral‐vector gene transfer.ResultsA high and stable long‐term expression was achieved after in vivo transfection of rat livers with AAV‐ITR‐containing plasmids. The expression course resembled that after AAV‐mediated gene transfer, and the expression was at least as high, and lasted as long, compared to recombinant AAV‐mediated gene transfer.ConclusionsWe consider AAV‐ITR‐containing plasmids as a simple and cost‐effective alternative to recombinant viral vectors, especially for liver‐directed gene therapy in rodents. With ongoing progress in gene transfer methods for naked DNA, these plasmids may also become a successful alternative to recombinant viral vectors in human gene therapy. Copyright © 2010 John Wiley & Sons, Ltd.
Reaction of 3‐iodoindole with 1,4‐naphthoquinones
Authors: Tanoue Yasuhiro , Teraoka Takashi , Kai Norihisa , Nagai Takeshi , Ushio Kazutoshi 
Published: August 2010

Abstract
magnified image The usefulness of 3‐iodoindole available for introduction of an indole unit is presented. The reaction of 3‐iodoindole with 2‐bromo(or methyl)‐1,4‐naphthoquinone in acetic acid gave 2‐bromo(or methyl)‐3‐(3‐indolyl)‐1,4‐naphthoquinone. On the other hand, the reaction of 3‐iodoindole with 2‐bromo‐1,4‐naphthoquinone in the presence of cesium carbonate in acetonitrile produced 2‐(1‐indolyl‐3‐iodo)‐1,4‐naphthoquinone. J. Heterocyclic Chem., (2010).
Synthesis of (±)‐trans‐hexahydropyrrolo[3,4‐d]oxazol‐2‐one and its derivatives by using N‐(tert‐butyloxycarbonyl)‐3‐pyrroline as precursor
Authors: Rajesh Tammana , Rao Sambangi Polinaini Narayana , Suresh Kopparthi , Madhusudhan Gutta , Mukkanti Kagga 
Published: August 2010

Abstract
magnified image Synthetic method for the preparation of (±)‐trans‐hexahydropyrrolo[3,4‐d]oxazol‐2‐one and its derivatives has been developed. By one route, an efficient preparation of these fused heterobicyclic moieties could be achieved, which are prepared by using N‐(tert‐butyloxycarbonyl)‐3‐pyrroline as precursor. These fused heterobicyclic systems could be useful to develop a series of 2‐oxazolidinone analogues. J. Heterocyclic Chem., (2010).
Difference and variation of the sef14 operon gene clusters in S. pullorum
Authors: Zhu C. H. , Wu J. , Chen W. W. , Hassan H. Musa , Zhu G. Q. 
Published: August 2010

AbstractSEF14 fimbriae are only found in some strains of serogroup‐D Salmonella such as S. enteritidis, suggesting that SEF14 fimbriae may affect serovar‐specific virulence traits. In this study, we found that prevalence of sefA, sefD and sefR genes in S. dublin and S. enteritidis was 100%. In 18 isolates of S. pullorum, the prevalence of sefA gene was 100%, while the prevalence of sefD and sefR genes was 38.9% (7/18), and 11 strains isolated after 1980s did not contain any gene sefD or sefR. Interestingly, among the 7 strains of S. pullorum before 1980s, the sefD sequence has a missing base pair at position 196 and caused open reading frame (ORF) shift, resulting in a stop codon (TAG) at position 71 amino acid residual (Leu of TTA at position 214–216 shift into stop codon of TAG at position 215–217). Unlike S. pullorum, all S. enteritidis and S. dublin tested could express SEF14 fimbriae in vitro. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Growth temperature associated protein expression and membrane fatty acid composition profiles of Salmonella enterica serovar Typhimurium
Authors: Balamurugan Sampathkumar 
Published: August 2010

AbstractTotal cellular proteins and fatty acid composition profiles of mid‐log phase cells of Salmonella enterica serovar Typhimurium grown at 8, 25, 37 or 42 °C were separated by 2D‐PAGE and FAME analysis. Growth temperature associated protein expression can be grouped into 3 thermal classes which include proteins whose expression is: I) optimal at 37 °C, meaning their expression peaked at 37 °C; II) up‐regulated with an increase in growth temperature; III) down‐regulated with increase in growth temperature; meaning their expression peaked at 8 °C. At higher growth temperatures, proteins belonging to the functional groups of amino acid transport and metabolism, nucleotide metabolism, energy metabolism and post‐translation modifications (chaperones) are present in substantially higher amounts. This increase in abundance is regulated in a temperature dependent manner. It is important to point out that proteins involved in energy metabolism observed in higher amounts at higher growth temperatures all belong to the glycolysis pathway, while at 8 °C they belonged to the TCA cycle. Increase in growth temperatures results in a decrease in membrane fatty acid unsaturation and an increase in saturated and cyclic fatty acids. These results provide an insight into the dynamic molecular and physiological responses of Salmonella Typhimurium during growth at different temperatures. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Purification of a toxic cysteine protease produced by pathogenic Aeromonas hydrophila isolated from rainbow trout
Authors: Liu Ping‐Chung , Chuang Wen‐Hsiao , Tu Chih‐Ching , Lee Kuo‐Kau 
Published: August 2010

AbstractAn extracellular lethal toxin produced by Aeromonas hydrophila strain RT860715K originally isolated from diseased rainbow trout (Oncorhynchus mykiss) was purified by using Fast Protein Liquid Chromatography system with hydrophobic interaction chromatography and anion exchange columns. The toxin was a cysteine protease, inhibited by L ‐cysteine, iodoacetic acid, N ‐ethylamleimide, P‐chloromercuibenzene‐sulfonic acid and N‐α‐p‐tosyl‐1‐lysine‐chloromethyl ketone (TLCK), and showed maximal activity at pH 6.0. The molecular weight of the purified enzyme proved to be 94 kDa as estimated by SDS‐PAGE. In addition, the toxin was also completely inhibited by HgCl2 but partially inhibited by ethylenediamine tetraacetic acid (EDTA) and CuCl2. Both the extracellular products of Aeromonas hydrophila RT860715K and the purified protease were lethal to rainbow trout (weighing 18 g) with LD50 values of 2.87 and 0.93 μg protein g–1 fish body weight, respectively. The addition of L‐cysteine completely inhibited the lethal toxicity of the purified protease, indicating that this cysteine protease was a lethal toxin produced by the bacterium. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Isolation and characterization of an extremely long tail Thermus bacteriophage from Tengchong hot springs in China
Authors: Lin Lianbing , Hong Wei , Ji Xiuling , Han Jian , Huang Li , Wei Yunlin 
Published: August 2010

AbstractThermus strains are regarded as models to investigate the mechanism of thermostability of thermophiles, and phages from Thermus are particularly interesting because of their way to regulate gene expression. In this research, a Thermus bacteriophage named TSP4 (Thermus Siphoviridae phage) was isolated from Tengchong hot springs in China, and characteristics of morphology, temperature for phage production, pH and organic solvent sensitivity, DNA restriction endonuclease digestion and protein composition of TSP4 were further studied. TSP4 belonged to the Siphoviridae family and had a hexagonal head of 73 nm in diameter, an extremely long and flexible tail of 785 nm in length and 10 nm in width. TSP4 was very stable at 65 °C and pH 7.6. The capsid was apparently devoid of lipid. By SDS‐PAGE, six protein bands were found in purified virions. Despite their exceptional habitats separated by thousands of kilometers, the characteristics of this thermophilic phage showed high similarity to Thermus siphoviruses P23‐45 and P74‐26 isolated from Kamchatka peninsula hot springs in the Far East, Russia. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
DFT and MP2 based quantum mechanical calculations and a theoretical vibrational spectroscopic investigation on roscovitine, a potential drug to treat cancers
Authors: Balci K. , Akkaya Y. , Akyuz S. , Palavan‐Unsal N. 
Published: August 2010

AbstractTheoretically possible stable conformers of free roscovitine molecule in its electronic ground state were searched by means of molecular dynamics and energy minimization calculations performed using the MM2 force field. Afterwards, geometry optimization and thermochemistry calculations were carried out at room temperature for each of the found minimum‐energy conformers using the MP2 and DFT based electronic structure methods and different Pople‐style basis sets. The results obtained from these calculations confirmed that the strong intramolecular hydrogen bonding between the purine‐nitrogen and hydroxyl‐hydrogen atoms plays an important role on the rigidity of roscovitine molecule and causes a dramatic reduction in the number of the possible stable conformers of this molecule at room temperature. Furthermore, the same calculation results also revealed that two of the found seven stable conformers are considerably more favorable in energy than the others and thus dominate the experimental room‐temperature spectra of the molecule. In the light of the theoretical vibrational spectral data obtained for these two conformers, a successful assignment of the fundamental bands observed in the experimental IR and Raman spectra recorded at room temperature for solid roscovitine and for its ethanol solution is given, and the effects of the substitution and intramolecular hydrogen bonding on the fundamental bands associated with purine and phenyl group vibrations are discussed in detail. In the fitting of the calculated harmonic wavenumbers to the corresponding experimental wavenumbers, two different scaling procedures, called ‘dual scale factors’ and ‘Scaled Quantum Mechanical Force Field (SQM FF) methodology’, were applied independently. Both procedures yielded results generally in good agreement with the experiment; however, the SQM FF methodology proved its superiority over the other. Copyright © 2010 John Wiley & Sons, Ltd.
Self‐association and hydrogen bonding of propionaldehyde in binary mixtures with water and methanol investigated by concentration‐dependent polarized Raman study and DFT calculations
Authors: Singh Dheeraj K. , Srivastava Sunil K. , Schlücker S. , Singh Ranjan K. , Asthana B. P. 
Published: August 2010

AbstractThe Raman spectra of neat propionaldehyde [CH3CH2CHO or propanal (Pr)] and its binary mixtures with hydrogen‐donor solvents, water (W) and methanol (M), [CH3CH2CHO + H2O] and CH3CH2CHO + CH3OH] with different mole fractions of the reference system, Pr varying from 0.1 to 0.9 at a regular interval of 0.1, were recorded in the ν(CO) stretching region, 1600–1800 cm−1. The isotropic parts of the Raman spectra were analyzed for both the cases. The wavenumber positions and line widths of the component bands were determined by a rigorous line‐shape analysis, and the peaks corresponding to self‐associated and hydrogen‐bonded species were identified. Raman peak at ∼1721 cm−1 in neat Pr, which has been attributed to the self‐associated species, downshifts slightly (∼1 cm−1) in going from mole fraction 0.9 to 0.6 in (Pr + W) binary mixture, but on further dilution it shows a sudden downshift of ∼7 cm−1. This has been attributed to the low solubility of Pr in W (∼30%), which does not permit a hydrogen‐bonded network to form at higher concentrations of Pr. A significant decrease in the intensity of this peak in the Raman spectra of Pr in a nonpolar solvent, n‐heptane, at high dilution (C = 0.05) further confirms that this peak corresponds to the self‐associated species. In case of the (Pr + M) binary mixture, however, the spectral changes with concentration show a rather regular trend and no special features were observed. Copyright © 2010 John Wiley & Sons, Ltd.
Exposure to traumatic events among treatment‐seeking, alcohol‐dependent women and men without PTSD
Authors: Johnson Candace S. , Heffner Jaimee L. , Blom Thomas J. , Anthenelli Robert M. 
Published: August 2010

AbstractThe authors examined lifetime exposure to a range of traumatic events in 106 abstinent, treatment‐engaged (85% residential; 15% outpatient), alcohol‐dependent women (n = 53) and men without current or lifetime posttraumatic stress disorder. Alcohol‐dependent women reported greater severity of childhood trauma, but similar lifetime exposure to traumatic events compared with men. Alcohol‐dependent women without cocaine abuse or TB (n = 10) reported greater severity of childhood trauma than women with (n = 43), and men with (n = 21) or without (n = 32) cocaine abuse or dependence. Results extend previously observed gender differences in trauma histories among alcohol‐dependent adults and point to potential gender‐ and substance‐specific drug coaddiction effects that may have been influenced by trauma exposure.
An analysis of hearing aid fittings in adults using cochlear implants and contralateral hearing aids
Authors: Harris Michael S. , Hay‐McCutcheon Marcia 
Published: August 2010

AbstractObjectives/Hypothesis:The objective of this study was to assess the appropriateness of hearing aid fittings within a sample of adult cochlear implant recipients who use a hearing aid in the contralateral ear (i.e., bimodal stimulation).Methods:The hearing aid gain was measured using real ear testing for 14 postlingually deaf English‐speaking adults who use a cochlear implant in the contralateral ear. Unaided and aided audiometric testing assessed the degree of functional gain derived from hearing aid use.Results:On average, the target to actual output level difference was within 10 dB only at frequencies of 750 Hz and 1,000 Hz. Only 1 of the 14 study participants had a hearing aid for which the majority of the tested frequencies were within 10 dB of the target gain. In addition, a greater amount of functional gain (i.e., the increase in unaided behavioral thresholds after amplification) was provided for lower frequencies than higher frequencies.Conclusions:Hearing aid settings in our sample were suboptimal and may be regarded as a contributing factor to the variability in bimodal benefit. Refining hearing aid fitting strategies tailored to the needs of the concurrent cochlear implant and hearing aid user is recommended. Laryngoscope, 2010
A randomized double‐blind controlled trial of phosphorylcholine‐coated tympanostomy tube versus standard tympanostomy tube in children with recurrent acute and chronic otitis media
Authors: Hong Paul , Smith Neil , Johnson Liane B. , Corsten Gerard 
Published: August 2010

AbstractObjective:To compare the postoperative complication rates of phosphorylcholine‐coated fluoroplastic tympanostomy tubes versus uncoated fluoroplastic tympanostomy tubes.Methods:A prospective randomized double‐blind controlled trial in children with recurrent acute otitis media and chronic otitis media with effusion; 240 children were randomized to receive a phosphorylcholine‐coated tube in one ear and an uncoated tube in the other. Postoperatively, patients were assessed at 2 weeks and 4, 8, 12, 18, and 24 months to ascertain the incidence of otorrhea, tube lumen blockage, and early extrusion.Results:Out of 240 children, 5 withdrew and 16 were lost to early follow‐up. The mean age was 43.8 months. There were no statistically significant differences in the incidence of postoperative otorrhea, tube blockage, and extrusion.Conclusions:Phosphorylcholine‐coated fluoroplastic ventilation tubes do not offer any advantages over uncoated standard fluoroplastic tympanostomy tubes. Laryngoscope, 2011
Uvulopalatal flap for obstructive sleep apnea: Short‐term and long‐term results
Authors: Neruntarat Chairat 
Published: August 2010

AbstractObjective:To study long‐term results in patients undergoing uvulopalatal flap (UPF) for obstructive sleep apnea (OSA).Design:Prospective, clinical trial at tertiary referral center.Material and Methods:A study was undertaken on 83 OSA patients with palatal obstruction based on radiography and physical findings. UPF was conducted to increase the airway space and data were analyzed.Results:Patients had a mean age of 36.5 ± 12.3 years and a mean body mass index (BMI) of 29.4 ± 4.3 kg/m2. The mean follow‐up was 54.2 ± 8.9 months, with a range of 48 to 62 months. The mean baseline apnea‐hypopnea index (AHI), short‐term AHI, and long‐term AHI were 45.6 ± 10.3, 13.4 ± 5.2, and 19.4 ± 5.1, respectively. The mean baseline lowest oxygen saturation (LSAT), short‐term LSAT, and long‐term LSAT were 82.6 ± 5.4%, 89.2 ± 4.8%, and 88.1 ± 3.2%, respectively. Forty‐three patients (51.8%) had long‐term success, and 15 patients(25.8%) had an increase in the AHI over the follow‐up period such that they were no longer considered success by traditional AHI criteria. Serious complications were not encountered.Conclusions:UPF is a safe and effective procedure that results in long‐term success for OSA. However, a regular follow‐up is important because some patients will relapse in the long term. Laryngoscope, 2011
Reevaluation of gastroesophageal reflux disease as a risk factor for laryngeal cancer
Authors: Francis David O. , Maynard Charles , Weymuller Ernest A. , Reiber Gayle , Merati Albert L. , Yueh Bevan 
Published: August 2010

AbstractObjectives/Hypothesis:The relationship between gastroesophageal reflux disease (GERD) and laryngeal cancer has not been fully elucidated. This case‐control study investigates whether GERD increases the odds of developing these malignancies.Study Design:Case‐control study.Methods:Rates of GERD among cases of laryngeal cancer identified in the Veterans Health Administration outpatient care files (year 2000–2006) were compared with controls. Cases (N = 14,449) were frequency matched 1:1 with controls. Multivariate logistic regression was used to determine the association between GERD and cancer.Results:After adjusting for tobacco and/or alcohol use, there was no association between GERD and laryngeal cancer (adjusted odds ratio, 1.01; 95% confidence interval, 0.92‐1.12, P =.780). Although an association was found when time from GERD diagnosis to malignancy was less than 3 months, it disappeared when this period was extended further.Conclusions:In this population, there was no increased risk of laryngeal cancer among patients with GERD. However, in subsite analysis, a possible relationship between GERD and glottic cancer was observed. Reverse causality must be considered in future studies assessing the relationship between reflux and laryngeal cancer to limit misclassification bias. Laryngoscope, 2011
Six-minute walk distance predicts mortality in liver transplant candidates
Authors: Carey Elizabeth J. , Steidley Eric D. , Aqel Bashar A. , Byrne Thomas J. , Mekeel Kristin L. , Rakela Jorge , Vargas Hugo E. , Douglas David D. 
Published: August 2010

Abstract
The 6-minute walk distance (6MWD) is a simple test measuring global physical function. It is commonly used to predict mortality in patients with cardiac and pulmonary diseases, but it is also useful in assessing the functional status of patients with a variety of other medical conditions. We sought to determine (1) the characteristics of the 6MWD in patients listed for liver transplantation (LT), (2) the existence of a relationship between the 6MWD and the quality of life, and (3) the relationship between the 6MWD and survival in LT candidates. The 6MWD was prospectively measured in all patients listed for LT. The 6MWD was determined when the listed Model for End-Stage Liver Disease (MELD) score was ≥15. Patients were followed until LT, death, removal from the wait list, or the end of the study period. Quality of life was assessed with the Short Form 36 (SF-36). In 121 patients, the mean 6MWD was 369 ± 122 m; it was not related to age, height, weight, body mass index, albumin level, or etiology of liver disease and showed a moderate correlation with the physical component score (PCS) on the SF-36 (r = 0.4) and a moderate inverse correlation with the native MELD score (r = −0.61). In an unadjusted analysis, a high native MELD score, a low 6MWD, and a low PCS were associated with mortality, with only the 6MWD retaining significance after adjustment for covariates. Each 100-m increase in the 6MWD was significantly associated with increased survival (hazard ratio = 0.48, P = 0.0001), with 6MWD < 250 m being associated with an increased risk of death (P = 0.0001). In conclusion, the 6MWD is significantly reduced in patients awaiting LT and is inversely correlated with the native MELD score. A pretransplant 6MWD < 250 m is a risk for death on the wait list. Liver Transpl 16:1373–1378, 2010. © 2010 AASLD.
Auxiliary partial orthotopic living donor liver transplantation with a small-for-size graft for congenital absence of the portal vein
Authors: Matsuura Toshiharu , Soejima Yuji , Taguchi Tomoaki 
Published: August 2010

Abstract
Congenital absence of the portal vein (CAPV) with an extrahepatic portosystemic shunt is a rare malformation; the completely absent type, Abernethy malformation type I, is especially rare. Liver transplantation for CAPV type I has been recently recognized as the only curative operation, but few reports have been published so far; meanwhile, auxiliary partial orthotopic liver transplantation (APOLT) has been proposed to be a very effective option, especially for pediatric patients. Here we present an 18-year-old adult patient with CAPV, asplenia, and an iliac shunt vessel who was managed successfully with APOLT using a small-for-size graft. To the best of our knowledge, this is the first adult patient who has experienced success with APOLT for CAPV. This is a feasible procedure: it not only fulfills the metabolic demands of the liver for adult patients but also potentially cures CAPV. Liver Transpl 16:1437–1439, 2010. © 2010 AASLD.
Effect of Poly(3‐octylthiophene) Doping on the Attachment and Proliferation of Osteoblasts
Authors: Rincón Charlene , Chen Chien‐Chiang , Meredith J. Carson 
Published: August 2010

AbstractThe effect of doping P3OT with ferric chloride on the attachment and proliferation of MC3T3‐E1 osteoblasts is reported. Cell density and area correlated strongly with doping concentration: cells were larger and exhibited better spreading as doping increased. Cells cultured on undoped P3OT showed a decrease in proliferation between 24 and 48 h followed by a recovery after 72 h. However, this trend diminished with increasing doping concentration, and disappeared completely at the highest dopant level investigated. Analysis of cell‐cell spatial distributions suggested that contact inhibition of proliferation occurred similarly on both undoped and doped P3OT. From these results, FeCl3‐doping had no significant deleterious effect on attachment or proliferation of osteoblasts in vitro.magnified image