bluuebunny/crossref_metadata_2025_split huggingface dataset:
The S. mansoni glycoprotein ω‐1 induces Foxp3 expression in NOD mouse CD4+ T cells
Authors: Zaccone Paola , Burton Oliver T. , Gibbs Sarah E. , Miller Nigel , Jones Frances M. , Schramm Gabriele , Haas Helmut , Doenhoff Michael J. , Dunne David W. , Cooke Anne 
Published: June 2011

AbstractImmunization with Schistosoma mansoni soluble antigen preparations protects non‐obese diabetic (NOD) mice against the development of type 1 diabetes. These preparations have long been known to induce Th2 responses in vitro and in vivo. Recently, two separate groups have reported that ω‐1, a well‐characterized glycoprotein in S. mansoni soluble egg antigens (SEA), which with IL‐4 inducing principle of S. mansoni eggs (IPSE/α‐1) is one of the two major glycoproteins secreted by live eggs, is a major SEA component responsible for this effect. We found that ω‐1 induces Foxp3 as well as IL‐4 expression when injected in vivo. We confirmed that ω‐1 conditions DCs to drive Th2 responses and further demonstrated that ω‐1 induces Foxp3+ T cells from NOD mouse naïve T cells. In contrast, IPSE/α‐1 did not drive Foxp3 responses. The in vitro development of Foxp3‐expressing T cells by ω‐1 was TGF‐β‐ and retinoic acid‐dependent. Our work, therefore, identifies ω‐1 as an important factor for the induction of Foxp3+ T cells by SEA in NOD mice.
A Tetranuclear Planar Hafnium Complex Containing O–Hf–S Moieties
Authors: Maratini Federica , Pandolfo Luciano , Rizzato Silvia , Albinati Alberto , Venzo Alfonso , Tondello Eugenio , Gross Silvia 
Published: June 2011

AbstractThe HfIV‐oxothiocluster, [Hf4(η2‐μ2‐SAc)4(η2‐μ2‐SAc)2(η2‐μ2‐BuO)4(η2‐BuO)2(μ3‐O)2], was synthesized by reaction of hafnium butoxide with thioacetic acid. The crystal structure shows the presence of a planar tetranuclear Hf4 core, in which the metal atoms are μ3‐O bridged and the thioacetate ligands coordinate the Hf ions both through a bridging and a chelating mode. The structural findings are confirmed by multinuclear NMR spectroscopic data.
Through‐Space J(P,P) and J(P,F) Spin–Spin Coupling in C1‐Symmetric Biaryl Diphosphanes
Authors: Bonnafoux Laurence , Ernst Ludger , Leroux Frédéric R. , Colobert Françoise 
Published: June 2011

AbstractThe first simultaneous 31P,31P and 31P,19F solution‐phase through‐space nuclear spin–spin coupling in biphenyl derivatives is reported. The magnitudes of the coupling constants are in the range 5.3–28.7 and 2.3–5.1 Hz, respectively. Their observation became possible through access to a recently developed class of C1‐symmetric biphenyl diphosphanes. We used this to prepare a series of model compounds for the study of the through‐space couplings. Various 1D and 2D techniques [COSY, NOESY, heteronuclear single quantum coherence (HSQC), HMBC, HSCQ‐TOCSY, selective 1H{31P}‐decoupling and iterative bandshape analysis] were applied to assign the 1H, 13C, 19F and 31P NMR spectra as completely as possible. X‐ray structure determinations confirmed the structural assignments and afforded P···P distances and torsion angles in the solid state. No simple correlation was found between the experimental J(31P,31P) values in solution and the relevant geometric parameters (P···P distance and torsional angle between the phosphorus lone pairs) in the solid state.
Facile Synthesis of Alternating Benzene–Pyrrole Oligomers by Cyclization of Propargylic Dithioacetals and Imines and Their Fluorescent Properties
Authors: Huang Tong‐Hui , Zhu Xiao‐Lei , Zhang Ai‐Dong , Tu Hai‐Yang 
Published: June 2011

AbstractAlternating benzene–heteroaryl oligomers possess fascinating optoelectronic properties and a wide range of applications. This article presents the facile synthesis of benzene–pyrrole oligomers with diverse functional groups and the elongated alternating heterocycle–benzene–pyrrole oligoaryls. The syntheses are based on a one‐pot, three‐step reaction of propargylic dithioacetals and imines. The subtle influence of functional groups, such as ether, ester, hydroxy, and dithiacetal groups on the peripheral benzene rings, on the reaction, has been explored and the reaction conditions were finely changed accordingly to achieve reasonable yields. The elongation could be accomplished by using a similar procedure. The fluorescent properties of original and elongated alternating benzene–pyrrole oligomers were determined. Theoretical calculations preliminarily reveal that the fluorescent intensity is closely related to the molecular geometry and to the HOMO/LUMO energy difference in the ground state.
Synthesis of Multi‐Functionalized Chromeno[2,3‐c]pyrrol‐9(2H)‐ones: Investigation and Application of Baker–Venkataraman Rearrangement Involved Reactions Catalyzed by 4‐(Dimethylamino)pyridine
Authors: Yu Yanjun , Hu Yun , Shao Weiyan , Huang Jianing , Zuo Yinglin , Huo Yingpeng , An Linkun , Du Jun , Bu Xianzhang 
Published: June 2011

AbstractAn efficient one‐pot synthesis of multi‐functionalized chromeno[2,3‐c]pyrrol‐9(2H)‐ones from 1,3‐diaryl‐1,3‐diketones and amino acids is described. The synthesis is based on the 4‐(dimethylamino)pyridine‐catalyzed Baker–Venkataraman rearrangement and subsequent reactions.
New Potent Fluorescent Analogues of Strigolactones: Synthesis and Biological Activity in Parasitic Weed Germination and Fungal Branching
Authors: Prandi Cristina , Occhiato Ernesto G. , Tabasso Silvia , Bonfante Paola , Novero Mara , Scarpi Dina , Bova Maria Elena , Miletto Ivana 
Published: June 2011

AbstractIn this work we report the synthesis of new fluorescent analogues of strigolactones, their spectroscopic properties and the evaluation of their biological activity both on seeds of Orobanche aegyptiaca and on the AM fungus Gigaspora margarita. The synthesis has been accomplished according to two different synthetic plans and allows the introduction of various substituents on the A and C rings of the framework, thus enabling access to bioactive molecules with different spectroscopic properties.
Proteomic analysis of a spring wheat cultivar in response to prolonged cold stress
Authors: Rinalducci Sara , Egidi Maria Giulia , Karimzadeh Ghasem , Jazii Ferdous Rastgar , Zolla Lello 
Published: June 2011

AbstractCold represents one of the major abiotic factors influencing plant growth and development worldwide. We analysed the long‐term responsiveness of an Iranian spring wheat (cv. Kohdasht) to cold from a proteomic point of view, in order to unravel the molecular mechanisms helping a cold‐sensitive cultivar to survive exposure to suboptimal temperatures. Plants were grown at 20 or 4°C until entering the reproductive stage and a cross‐comparison on the leaf proteomes was performed. Quantitative analyses on protein alterations occurring upon low‐temperature exposure showed a reinforcement in ascorbate recycling (dehydroascorbate reductase, ascorbate peroxidase) and protein processing (proteasome subunit, cysteine proteinase), as well as the accumulation of the enzyme devoted to tetrapyrrole resynthesis (glutamate semialdehyde aminomutase). In contrast, among proteins down‐regulated after cold stress, we could identify some key Krebs cycle enzymes (isocitrate dehydrogenase, malate dehydrogenase), together with many photosynthesis‐related proteins (oxygen‐evolving complex proteins, ATP synthase subunits, ferredoxin NADPH oxidoreductase and some Calvin cycle enzymes). Physiological and biochemical parameters (such as shoot apex dissection, chlorophyll, proline and sugar content determination) sustained proteomics findings allowing the present research to contribute to the current knowledge on these long‐term responses, which may be crucial to stress adaptation under field conditions.
Monitoring cytoplasmic protein complexes with blue native gel electrophoresis and stable isotope labelling with amino acids in cell culture: Analysis of changes in the 20S proteasome
Authors: Couttas Timothy A. , Raftery Mark J. , Erce Melissa A. , Wilkins Marc R. 
Published: June 2011

AbstractAnalysis of protein complexes is of increasing interest in the field of proteomics. A challenge is to develop methods for monitoring changes in the quantity and subunit composition of protein complexes on a proteome‐wide scale. Here, we describe the combination of 1‐D blue native polyacrylamide gel electrophoresis (BN‐PAGE) with stable isotope labelling of amino acids in cell culture (SILAC) and tandem mass spectrometry (MS/MS). Cleared lysates from normal and perturbed samples, one incorporating heavy stable isotopes and the other light isotopes, are co‐separated by blue native PAGE and then analysed and quantitated with MS/MS and appropriate software. This permits the analysis of cytoplasmic complexes. To demonstrate this technique, we explored how the 20S proteasome changes when the Pre9/α3 subunit, the only non‐essential subunit of this complex, was deleted. Our results showed that ΔPre9/α3 cells can form the 20S proteasome complex, although with reduced efficiency. This involves an increase in expression of the α4 subunit. Our findings suggest this technique as an approach for the study of quantitative and qualitative differences in protein complexes, from cleared cell lysates.
An allelic ladder based upon reference alleles for mtDNA SNP analysis using the SNaPshot technique
Authors: Köhnemann Stephan , Pennekamp Petra , Pfeiffer Heidi 
Published: June 2011

AbstractThe analysis of mitochondrial DNA (mtDNA) single‐nucleotide polymorphisms (SNPs) using the SNaPshot technique (Applied Biosystems) is a fast and sensitive method for the reliable identification of disease‐associated mtDNA SNPs, genetic ancestry mtDNA SNPs and forensically important mtDNA SNPs. The detection of many SNPs in one multiplex PCR and one subsequent multiplex minisequencing reaction is challenging for laboratories who want to establish this technique, due to the problem that there is no allelic ladder available for mtDNA SNP analysis via SNaPshot technique. Normally, the laboratory has to invent long‐term testing and studies. The interpretation of false and correct alleles is up to some specialists knowing the expected and the estimated size of each allele SNP. We here present a protocol to assemble up to 84 alleles of 42 different mtDNA SNPs in an allelic ladder that is based upon reference alleles. We recommend using allelic ladders/reference alleles for SNP analysis to maintain high‐quality analysis standards.
Entrapment of a Trigonopsis variabilis D‐amino acid oxidase variant F54Y for oxidative deamination of cephalosporin C
Authors: Wong Kin‐Sing , Fong Wing‐Ping , Tsang Paul Wai‐Kei 
Published: June 2011

AbstractTrigonopsis variabilis D‐amino acid oxidase (TvDAAO) is an enzyme used in the industrial bioconversion of cephalosporin C (CPC) into 7‐aminocephalosporanic acid, a crucial biosynthetic nucleus for a wide spectrum of semi‐synthetic cephem antibiotics. Using homology modeling and site‐directed mutagenesis, we have previously shown that the TvDAAO variant F54Y possesses improved catalytic activity and thermostability. To further explore its industrial application, the conditions for immobilization of the enzyme were examined in the present investigation. The results showed that entrapment in a calcium alginate (Ca‐alginate) matrix using 2% alginate, 500 mM CaCl2, and 15 min stabilization appeared to be optimal for the immobilization of F54Y. The entrapped enzyme allowed complete CPC conversion. The entrapped enzyme also showed good operational stability and retained at least 90% of its original activity after 20 reaction cycles. To conclude, the entrapment of F54Y in Ca‐alginate appeared to be a simple and efficient biocatalysis system with potential application in the antibiotics industry.
Cyclic performance of large‐scale corroded reinforced concrete beams
Authors: Ou Yu‐Chen , Tsai Li‐Lan , Chen Hou‐Heng 
Published: June 2011

SUMMARYNine large‐scale beam specimens were constructed. Of which, one was used as the control, whereas the other eight ones were divided into four sets. Each set had two specimens and was subjected to accelerated corrosion using an imposed current for the same time interval. Following the corrosion, a specimen in each set was tested using cyclic loading to examine the seismic performance, whereas the other one was demolished to examine the extent of corrosion. Cyclic loading results indicated that with an increasing corrosion level, the ultimate drift, ductility, plastic rotation capacity, and energy dissipation of the beams initially increased and later decreased. The failure mode switched from flexural failure, largely owing to buckling of the longitudinal reinforcement to flexural‐shear failure, which is mainly caused by fracturing of the transverse reinforcement. Corrosion increased shear deformation and the spread of plasticity of the plastic hinge region. The residual flexural strength, as estimated by an empirical equation based on the maximum pit depth in the longitudinal reinforcement, closely corresponds to experimental values. Furthermore, the residual shear strength estimated based on the minimum reduced cross‐sectional area of transverse reinforcement correlates better with the experimental observations than that based on the weight loss. Copyright © 2011 John Wiley & Sons, Ltd.
The Relationship Among Compulsive Buying, Compulsive Internet Use and Temperament in a Sample of Female Patients with Eating Disorders
Authors: Claes Laurence , Müller Astrid , Norré Jan , Van Assche Leen , Wonderlich Steve , Mitchell James E. 
Published: June 2011

AbstractThe aim of the present study was to investigate the association among compulsive buying (CB), compulsive internet use (CIU) and reactive/regulative temperament in a sample of 60 female patients with eating disorders. All patients were assessed by means of the Compulsive Buying Scale, the CIU scale, the Eating Disorder Inventory—2, the Behavioral Inhibition System/Behavioral Activation System scales, the Dimensional Assessment of Personality Pathology and the effortful control scale of the Adult Temperament Questionnaire. The results showed a positive association between CB and CIU, both categorized as impulse control disorders, not otherwise specified. Both CB and CIU showed significantly positive correlations with emotional lability, excitement seeking and lack of effortful control (more specifically lack of inhibitory and lack of activation control). The implication of these findings for the treatment of both disorders will be discussed. Copyright © 2011 John Wiley & Sons, Ltd and Eating Disorders Association.
Coupling of methylmercury uptake with respiration and water pumping in freshwater tilapia Oreochromis niloticus
Authors: Wang Rui , Wong Ming-Hung , Wang Wen-Xiong 
Published: June 2011

Abstract
The relationships among the uptake of toxic methylmercury (MeHg) and two important fish physiological processes—respiration and water pumping—in the Nile tilapia (Oreochromis niloticus) were explored in the present study. Coupled radiotracer and respirometric techniques were applied to measure simultaneously the uptake rates of MeHg, water, and oxygen under various environmental conditions (temperature, dissolved oxygen level, and water flow). A higher temperature enhanced MeHg influx and the oxygen consumption rate but had no effect on the water uptake, indicating the influence of metabolism on MeHg uptake. The fish showed a high tolerance to hypoxia, and the oxygen consumption rate was not affected until the dissolved oxygen concentration decreased to extremely low levels (below 1 mg/L). The MeHg and water uptake rates increased simultaneously as the dissolved oxygen level decreased, suggesting the coupling of water flux and MeHg uptake. The influence of fish swimming performance on MeHg uptake was also investigated for the first time. Rapidly swimming fish showed significantly higher uptake rates of MeHg, water, and oxygen, confirming the coupling relationships among respiration, water pumping, and metal uptake. Moreover, these results support that MeHg uptake is a rate-limiting process involving energy. Our study demonstrates the importance of physiological processes in understanding mercury bioaccumulation in fluctuating aquatic environments. Environ. Toxicol. Chem. 2011;30:2142–2147. © 2011 SETAC
A linear solvation energy relationship model of organic chemical partitioning to particulate organic carbon in soils and sediments
Authors: Kipka Undine , Di Toro Dominic M 
Published: June 2011

Abstract
Predicting the association of contaminants with particulate organic matter in the environment is critical in determining the fate and bioavailability of chemicals. A ubiquitous measure of contaminant association with soil and sediment particulate organic matter is the organic carbon partition coefficient KOC. Chemical class-specific models relating the KOC to the octanol–water partition coefficient KOW have been used to predict the partitioning to organic carbon in the water column and sediment for nonpolar hydrophobic pollutants and some polar pollutants. A single linear solvation energy relationship (LSER) is proposed as a simpler and chemically based alternative for predicting KOC for a more diverse set of compounds. A chemically diverse set of KOC data is used to obtain a more robust and more universally representative model of organic carbon partitioning than previously available LSER models. The resulting model has a root mean square error (RMSE) of prediction for log KOC of RMSE = 0.48 for the fitted data set and RMSE = 0.55 for an independent data set. An analysis of LSER coefficients highlights the relative importance of hydrogen bonding interactions. Environ. Toxicol. Chem. 2011;30:2013–2022. © 2011 SETAC
Effect of zinc on diversity of riverine benthic macroinvertebrates: Estimation of safe concentrations from field data
Authors: Iwasaki Yuichi , Kagaya Takashi , Miyamoto Ken-ichi , Matsuda Hiroyuki , Sakakibara Mayu 
Published: June 2011

Abstract
We conducted field surveys at 25 sites in three Japanese catchments to provide conservative estimates of the safe concentration of zinc (Zn) for the protection of riverine macroinvertebrate diversity. The relationships between the Zn concentration and six macroinvertebrate metrics for taxon richness were determined by using regression analysis; this included a piecewise regression model, where two lines are joined at an unknown point. For each metric the piecewise regression model with a zero slope below a threshold concentration was selected as the best model to explain the influence of Zn. Under the assumption that macroinvertebrate diversity reductions of &lt;10% are acceptable, the safe concentrations of Zn were estimated to be 84, 115, 84, 80, 85, and 70 µg/L for total taxon richness, Ephemeroptera, Plecoptera, and Trichoptera (EPT) richness, mayfly richness, caddisfly richness, chironomid richness, and estimated total taxon richness at the riffle scale, respectively. These concentrations are more than twice the water quality standard for Zn in Japan (30 µg/L), suggesting that the standard is likely overprotective for macroinvertebrate diversity. Field studies are useful for evaluating the level of protectiveness of safe concentrations (water quality standards) based on individual-level effects from laboratory toxicity tests, and this evaluation process will have a crucial role in implementing more purpose-driven ecological risk managements that aim to protect natural populations and communities. Environ. Toxicol. Chem. 2011;30:2237–2243. © 2011 SETAC
Extended homotopy perturbation method and the axisymmetric flow past a porous stretching sheet
Authors: Al‐Mdallal Qasem M. , Syam Muhammed I. , Donald Ariel P. 
Published: June 2011

SUMMARYThe extended homotopy perturbation method, which is an extension of the celebrated homotopy perturbation method (HPM), is applied to obtain a solution to the problem of the steady, laminar, axisymmetric flow of a viscous, incompressible fluid past a porous stretching sheet. The solution so obtained is totally analytical and is expressible in terms of the cross‐flow velocity of the fluid past the stretching sheet. Its hallmark is that it does not depend upon computation of any auxiliary parameter for enlarging the convergence region of the solution. Rather, it calculates the solution automatically adjusting the scaling factor of the independent similarity variable normal to the sheet. The results obtained by the extended HPM are in excellent agreement with the exact numerical solution. Also, an asymptotic solution valid for large suction parameter is developed, which matches well with the exact solution even for moderate values of the suction parameter. Copyright © 2011 John Wiley & Sons, Ltd.
Consistent t(1;10) with rearrangements of TGFBR3 and MGEA5 in both myxoinflammatory fibroblastic sarcoma and hemosiderotic fibrolipomatous tumor
Authors: Antonescu Cristina R. , Zhang Lei , Nielsen G.Petur , Rosenberg Andrew E. , Cin Paola Dal , Fletcher Christopher D. M. 
Published: June 2011

AbstractDespite their shared predilection for superficial soft tissue of distal extremities and frequent local recurrences, myxoinflammatory fibroblastic sarcoma (MIFS) and hemosiderotic fibrolipomatous tumor (HFLT) have distinct morphologic appearances. Recent studies have identified an identical t(1;10)(p22;q24) in five cases of MIFS and two of HFLT, as well as common amplifications on 3p11‐12. To investigate further their potential relationship and to determine the incidence of t(1;10) in a larger cohort, we subjected seven MIFS, 14 HFLT, and three cases with mixed morphology, to molecular and cytogenetic analysis. Fluorescence in situ hybridization (FISH) analysis for rearrangements of TGFBR3 on 1p22 and of MGEA5 on 10q24 was performed in all cases, whereas the status of VGLL3 gene amplification on 3p12.1 was investigated in 12 cases. Conventional karyotyping was performed in one HFLT and two cases with mixed MIFS/HFLT histology. Overall 83% of cases showed rearrangements in both TGFBR3 and MGEA5. All three cases with mixed features of MIFS and HFLT were positive. Cytogenetic analysis performed in three cases confirmed an unbalanced der(10)t(1;10)(p22;q24). VGLL3 gene amplification was noted in 10/12 cases of both histologies. The high incidence of t(1;10) in MIFS and HFLT reinforces a shared pathogenetic relationship. Furthermore, the co‐existence of both components either synchronously or metachronously in a primary or subsequent recurrence, suggest either different morphologic variants or different levels of tumor progression of a single biologic entity. FISH analysis for TGFBR3 and MGEA5 rearrangements can be applied as a reliable diagnostic molecular test when confronted with limited material or a challenging diagnosis. © 2011 Wiley‐Liss, Inc.
Parafibromin immunohistochemical staining to differentiate parathyroid carcinoma from parathyroid adenoma
Authors: Kim Hee Kyung , Oh Young Lyun , Kim Seok‐Hyung , Lee Dong Youn , Kang Ho‐Cheol , Lee Ji In , Jang Hye Won , Hur Kyu Yeon , Kim Jae Hyeon , Min Yong Ki , Chung Jae Hoon , Kim Sun Wook 
Published: June 2011

AbstractBackgroundParafibromin is a protein encoded by the HRPT2 oncosuppressor gene, and the expression is reported to be decreased or absent in parathyroid carcinomas.MethodsA total of 26 tumor specimens from 18 patients with adenoma and 8 patients with carcinoma were immune‐stained with an antibody against parafibromin.ResultsParafibromin immunostaining showed strong positivity in 17 of 18 adenomas. Negative staining was noted in 3 of 8 carcinomas, and weak positivity was found in 3 of 8 carcinomas. The remaining 2 cases of carcinoma showed strong positivity. The loss of parafibromin expression (negative or weak positivity) demonstrated 94.4% specificity in the diagnosis of parathyroid carcinomas. Relapses or distant metastases of carcinoma occurred only in cases in which there was a loss of parafibromin immunostaining.ConclusionsLoss of parafibromin immunostating showed promising results in the differential diagnosis of parathyroid carcinoma from adenoma and may also serve as a prognostic marker. © 2011 Wiley Periodicals, Inc. Head Neck, 2012