bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interaction of the Vaccinia Virus RNA Polymerase-Associated 94-Kilodalton Protein with the Early Transcription Factor
Authors: Yang Zhilong , Moss Bernard 
Published: September 2009

ABSTRACT

A multisubunit RNA polymerase (RPO) encoded by vaccinia virus (VACV), in conjunction with specific factors, transcribes early, intermediate, and late viral genes. However, an additional virus-encoded polypeptide referred to as the RPO-associated protein of 94 kDa (RAP94) is tightly bound to the RPO for the transcription of early genes. Unlike the eight RPO core subunits, RAP94 is synthesized exclusively at late times after infection. Furthermore, RAP94 is necessary for the packaging of RPO and other components needed for early transcription in assembling virus particles. The direct association of RAP94 with NPH I, a DNA-dependent ATPase required for transcription termination, and the multifunctional poly(A) polymerase small subunit/2′-
O
-methyltransferase/elongation factor was previously demonstrated. That RAP94 provides a structural and functional link between the core RPO and the VACV early transcription factor (VETF) has been suspected but not previously demonstrated. Using VACV recombinants that constitutively or inducibly express VETF subunits and RAP94 with affinity tags, we showed that (i) VETF associates only with RPO containing RAP94 in vivo and in vitro, (ii) the association of RAP94 with VETF requires both subunits of the latter, (iii) neither viral DNA nor other virus-encoded late proteins are required for the interaction of RAP94 with VETF and core RPO subunits, (iv) different domains of RAP94 bind VETF and core subunits of RPO, and (v) NPH I and VETF bind independently and possibly simultaneously to the N-terminal region of RAP94. Thus, RAP94 provides the bridge between the RPO and proteins needed for transcription initiation, elongation, and termination.
Petrogenesis of the 1.9 Ga mafic hanging wall sequence to the Flin Flon, Callinan, and Triple 7 massive sulphide deposits, Flin Flon, Manitoba, CanadaThis is a companion paper to DeWolfe, Y.M., Gibson, H.L., Lafrance , B., and Bailes, A.H. 2009. Volcanic reconstruction of Paleoproterozoic arc volcanoes: the Hidden and Louis formations, Flin Flon, Manitoba, Canada. Canadian Journal of Earth Sciences, 46: this issue.
Authors: DeWolfe Y. M. , Gibson H. L. , Piercey S. J. 
Published: September 2009

A detailed study of the geochemical and isotopic characteristics of the volcanic rocks of the Hidden and Louis formations, which make up the hanging wall to the volcanogenic massive sulphide deposits at Flin Flon, Manitoba, was carried out on a stratigraphically controlled set of samples. The stratigraphy consists of the lowermost, dominantly basaltic, Hidden formation, and the overlying, dominantly basaltic, Louis formation. Of importance petrogenetically, is the 1920 unit a basaltic andesite with Nb/Thmn = 0.54–0.62, εNd(1.9Ga) = +3.6–+5.9, εHf(1.9Ga) = +8.5–+9.6, and 204Pb/206Pb = 23.9. The basaltic flows that dominate the Hidden formation have Nb/Thmn = 0.16–0.29, εNd(1.9Ga) = +1.7–+4.4, εHf(1.9Ga) = +7.0–+11.8 and 204Pb/206Pb = 16.9–18.6). The Carlisle Lake basaltic–andesite (top of Hidden formation) is characterized by Nb/Thmn = 0.16–0.14, and 204Pb/206Pb = 21.4. The rhyodacitic Tower member (bottom of Louis formation) has Nb/Thmn = 0.23, εNd1.9Ga = +4.6, εHf1.9Ga = +9.3, and 204Pb/206Pb = 22.2. The basaltic flows that dominate the Louis formation have Nb/Thmn = 0.18–0.25, εNd(1.9Ga) = +3.6–+4.2, εHf(1.9Ga) = +8.4–+11.3 and 204Pb/206Pb = 17.9. The Hidden and Louis formations show dominantly transitional arc tholeiite signatures, with the 1920 unit having arc tholeiite characteristics. It is interpreted to have formed through extensive fractional crystallization of differentiated magmas at shallow levels in oceanic crust. Given the geological, geochemical, and isotopic characteristics of the Hidden and Louis formations, they are interpreted to represent subducted slab metasomatism with minor contamination from subducted sediments.
Relationship between bruchid resistance and seed mass in mungbean based on QTL analysis
Authors: Mei L. , Cheng X. Z. , Wang S. H. , Wang L. X. , Liu C. Y. , Sun L. , Xu N. , Humphry M. E. , Lambrides C. J. , Li H. B. , Liu C. J. 
Published: September 2009

Bruchids (Coleoptera: Bruchidae) can cause serious damage to mungbean and several other leguminous crops and there is a strong association between small seed size and bruchid resistance. In investigating the feasibility of breeding large-seeded cultivars with high levels of bruchid resistance, we studied the relationship between these two traits by QTL analysis. A major locus conferring resistance to Callosobruchus chinensis was identified from a wild mungbean genotype, ‘ACC41’ (belonging to Vigna radiata var. sublobata), collected in Australia. The proportion of the C. chinensis resistance response that could be attributed to this single QTL varied among four different resistance assays. The highest value reached was 98.5%, suggesting that bruchid resistance in this genotype is likely conditioned by this single locus. The QTL was robust and its detection was not affected by the use of different sources of the insect, different lengths and conditions of seed storage, or different bruchid resistance assay methods. This bruchid resistance QTL was coincident with one of the loci conferring seed mass detected from the three seed sources produced in Australia. However, such a co-location was not detected for the seed source produced in China. Covariance analysis revealed a complex relationship between seed mass and bruchid resistance. Nevertheless, the effect of the bruchid resistance QTL remained highly significant for all four assays after the effect of seed mass was accounted for. These results, together with the relationship between the bruchid resistance QTL identified in this study and a second one detected previously in a wild mungbean genotype from Madagascar, are discussed.
Comparative analysis of theBrassica oleraceagenetic map and theArabidopsis thalianagenome
Authors: Kaczmarek Malgorzata , Koczyk Grzegorz , Ziolkowski Piotr A. , Babula-Skowronska Danuta , Sadowski Jan 
Published: September 2009

We further investigated genome macrosynteny for Brassica species and Arabidopsis thaliana . This work aimed at comparative map construction for B. oleracea and A. thaliana chromosomes based on 160 known A. thaliana probes: 147 expressed sequence tags (ESTs) and 13 full-length cDNA clones. Based on an in silico study of the A. thaliana genome, most of the selected ESTs (83%) represented unique or low-copy genes. We identified conserved segments by the visual inspection of comparative data with a priori assumptions, and established their significance with the LineUp algorithm. Evaluation of the number of B. oleracea gene copies per A. thaliana EST revealed a fixed upward trend. We established a segregation distortion pattern for all genetic loci, with particular consideration of the type of selection (gametic or zygotic), and discuss its possible impact on genetic map construction. Consistent with previous reports, we found evidence for numerous chromosome rearrangements and the genome fragment replication of B. oleracea that have taken place since the divergence of the two species. Also, we found that over 54% of the B. oleracea genome is covered by 24 segments conserved with the A. thaliana genome. The average conserved segment is composed of 5 loci covering 19.3 cM in the B. oleracea genetic map and 2.42 Mb in the A. thaliana physical map. We have also attempted to use a unified system of conserved blocks (previously described) to verify our results and perform a comprehensive comparison with other Brassica species.
Localisation of quantitative trait loci for quality attributes in a doubled haploid population of wheat (Triticum aestivumL.)
Authors: Raman R. , Allen H. , Diffey S. , Raman H. , Martin P. , McKelvie K. 
Published: September 2009

Selection of wheat germplasm for a range of quality traits has been a challenging exercise because of the cost of testing, the variation within testing data, and a poor understanding of the underlying genetics. The objective of this study was to identify quantitative trait loci (QTLs) underlying quality traits in wheat. A doubled haploid population comprising 190 lines from Chara/WW2449 was grown in two different environments and evaluated for various quality traits. A molecular map comprising 362 markers based upon simple sequence repeat, sequence tagged microsatellite, glutenin, and DArT loci was constructed and subsequently exploited to identify QTLs using a whole-genome approach. Fifteen QTLs that were consistent in the two different environments were identified for thousand kernel mass, grain protein content, milling yield, flour protein content, flour colour, flour water absorption, dough development time, dough strength (extensograph height and resistance at 5 cm), and dough extensibility (extensograph length) using the whole genome average interval mapping approach. The amount of genetic variation explained by individual QTLs ranged from 3% to 49%. A number of QTLs associated with dough strength, dough extensibility, dough development time, and flour water absorption were located close to the glutenin Glu-B1 locus on chromosome 1B. Identification of the chromosomal location and effect of the QTLs influencing wheat quality may hasten the development of superior wheats for target markets via marker-assisted selection.
High-dose diazepam facilitates core cooling during cold saline infusion in healthy volunteers
Authors: Hostler David , Northington William E. , Callaway Clifton W. 
Published: September 2009

Studies have suggested that inducing mild hypothermia improves neurologic outcomes after traumatic brain injury, major stroke, cardiac arrest, or exertional heat illness. While infusion of cold normal saline is a simple and inexpensive method for reducing core temperature, human cold-defense mechanisms potentially make this route stressful or ineffective. We hypothesized that intravenous administration of diazepam during a rapid infusion of 30 mL·kg–1 of cold (4 °C) 0.9% saline to healthy subjects would be more comfortable and reduce core body temperature more than the administration of cold saline alone. Fifteen subjects received rapidly infused cold (4 °C) 0.9% saline. Subjects were randomly assigned to receive, intravenously, 20 mg diazepam (HIGH), 10 mg diazepam (LOW), or placebo (CON). Main outcomes were core temperature, skin temperature, and oxygen consumption. Data for the main outcomes were analyzed with generalized estimating equations to identify differences in group, time, or a group × time interaction. Core temperature decreased in all groups (CON, 1.0 ± 0.2 °C; LOW, 1.4 ± 0.2 °C; HIGH, 1.5 ± 0.2 °C), while skin temperature was unchanged. Mean (95% CI) oxygen consumption was 315.3 (253.8, 376.9) mL·kg–1·min–1 in the CON group, 317.9 (275.5, 360.3) in the LOW group, and 226.1 (216.4, 235.9) in the HIGH group. Significant time and group × time interaction was observed for core temperature and oxygen consumption (p < 0.001). Administration of high-dose diazepam resulted in decreased oxygen consumption during cold saline infusion, suggesting that 20 mg of intravenous diazepam may reduce the shivering threshold without compromising respiratory or cardiovascular function.
Effects of Ramadan fasting on physical performance and metabolic, hormonal, and inflammatory parameters in middle-distance runners
Authors: Chennaoui Mounir , Desgorces François , Drogou Catherine , Boudjemaa Bechir , Tomaszewski Armand , Depiesse Frédéric , Burnat Pascal , Chalabi Hakim , Gomez-Merino Danielle 
Published: September 2009

The Ramadan fasting (RF) period is associated with changes in sleep habits and increased sleepiness, which may affect physical performance in athletes, and may induce metabolic, hormonal, and inflammatory disturbances. In 8 middle-distance athletes (25.0 ± 1.3 years), a maximal aerobic velocity (MAV) test was performed 5 days before RF (day –5), and on days 7 and 21 of RF. The same days, saliva samples were collected to determine cortisol and testosterone concentrations before and after the MAV test. Blood samples were collected before RF (P1), at the end of RF (P2), and 1 week post RF (P3). Plasma levels of interleukin (IL)-6, a mediator of sleepiness and energy availability, were determined. We also evaluated changes in metabolic and hormonal parameters, mood state, and nutritional and sleep profiles. During RF, mean body mass and body fat did not statistically change. Compared with day –5, MAV values decreased at days 7 and 21 (p < 0.05, respectively), while testosterone/cortisol ratio values did not change significantly. Nocturnal sleep time and energy intake were lower at day 21 than before RF (day 0/P1) (p < 0.05). At the end of RF (day 31), the fatigue score on the Profile of Mood States questionnaire was increased (p < 0.001). For P2 vs. P1, IL-6 was increased (1.19 ± 0.25 vs. 0.51 ± 0.13 pg·mL–1; p < 0.05), melatonin levels were decreased (p < 0.05), and adrenalin and noradrenalin were increased (p < 0.01 and p < 0.001, respectively). At 7 days post RF, all parameters recovered to pre-RF values. In conclusion, RF is accompanied by significant metabolic, hormonal, and inflammatory changes. Sleep disturbances, energy deficiency, and fatigue during RF may decrease physical performance in Muslim athletes who maintain training. Reduction of work load and (or) daytime napping may represent adequate strategies to counteract RF effects for Muslim athletes.
Child–adult differences in muscle strength and activation pattern during isometric elbow flexion and extension
Authors: Falk Bareket , Usselman Charlotte , Dotan Raffy , Brunton Laura , Klentrou Panagiota , Shaw Jay , Gabriel David 
Published: September 2009

Muscle strength and activation were compared in boys and men during maximal voluntary elbow flexion and extension contractions. Peak torque, peak rate of torque development (dτ/dτmax), rate of muscle activation, and electromechanical delay (EMD) were measured in 15 boys (aged 9.7 ± 1.6 years) and 16 men (aged 22.1 ± 2.8 years). During flexion, peak torque was significantly lower in boys than in men (19.5 ± 5.8 vs. 68.5 ± 11.0 Nm, respectively; p < 0.05), even when controlling for upper-arm cross-sectional area (CSA), and peak electromyography activity. Boys also exhibited a lower normalized dτ/dτmax(7.2 ± 1.7 vs. 9.5 ± 1.6 (Nm·s–1)·(Nm–1), respectively; p < 0.05) and a significantly longer EMD (75.5 ± 28.4 vs. 47.6 ± 17.5 ms, respectively). The pattern was similar for extension, except that group differences in peak torque were no longer significant when normalized for CSA. These results suggest that children may be less able to recruit or fully utilize their higher-threshold motor units, resulting in lower dimensionally normalized maximal torque and rate of torque development.
Does habitual dietary intake influence myofiber hypertrophy in response to resistance training? A cluster analysis
Authors: Thalacker-Mercer Anna E. , Petrella John K. , Bamman Marcas M. 
Published: September 2009

Although resistance exercise training (RT) is a common intervention to stimulate muscle protein synthesis and increase skeletal muscle mass, the optimal daily protein and total energy intakes sufficient to support RT-mediated muscle growth are as yet unclear. Further, the efficacy of RT varies widely among adults of all ages and whether this is attributable to interindividual differences in nutrition is not known. To determine if self-selected daily intake of macronutrients and specific components of dietary protein and fat are predictive of the magnitude of RT-mediated muscle growth, detailed 4-day dietary records were analyzed on 60 subjects previously clustered (K-means cluster analysis) as non-, modest, and extreme responders (non, n = 16; mod, n = 29; xtr, n = 15), based on the magnitudes of change in vastus lateralis myofiber cross-sectional area following a 16-week, 3-day-per-week, high-intensity RT. Despite the marked contrast between 60% myofiber hypertrophy in xtr and zero growth in non, we found no differences among response clusters in daily intakes of energy (mean ± SEM: non 102 ± 8; mod 111 ± 6; xtr 109 ± 5 kJ·kg–1·day–1), protein (non 0.97 ± 0.08; mod 1.07 ± 0.07; xtr 1.05 ± 0.06 g·kg–1·day–1), carbohydrate (non 3.02 ± 0.24; mod 3.18 ± 0.20; xtr 3.14 ± 0.17 g·kg–1·day–1), and fat (non 0.95 ± 0.09; mod 1.05 ± 0.08; xtr 1.03 ± 0.08 g·kg–1·day–1), which generally met or exceeded dietary recommendations. There were no cluster differences in intakes of branched chain amino acids known to stimulate muscle protein synthesis. Using the novel K-means clustering approach, we conclude from this preliminary study that protein and energy intakes were sufficient to facilitate modest and extreme muscle growth during RT and intrinsic or extrinsic factors other than nutrient ingestion apparently impaired the anabolic response in nonresponders.
Dietary patterns associated with glycemic index and glycemic load among Alberta adolescents
Authors: Forbes Laura E. , Storey Kate E. , Fraser Shawn N. , Spence John C. , Plotnikoff Ronald C. , Raine Kim D. , Hanning Rhona M. , McCargar Linda J. 
Published: September 2009

The purpose of this study was to assess the dietary glycemic index (GI) and glycemic load (GL) of adolescents, based on a Web-based 24-h recall, and to investigate dietary predictors of GI and GL. In addition, the relationship between GI and GL and weight status was examined. A Web-based 24-h recall was completed by 4936 adolescents, aged 9–17 years; macronutrient and food group intakes were assessed using the ESHA Food Processor, the Canadian Nutrient File, and Canada’s Food Guide. Dietary GI and GL were calculated based on published GI values for foods. Students provided self-reported height and mass. Multiple regression models assessed the ability of food group choices and food behaviours to predict GI and GL. Mean GI was 55 for girls and 56 for boys. Mean GL was 128 for girls and 168 for boys. Food group choices explained 26% of the variation in GI (p < 0.01) and 84% of the variation in GL (p < 0.01). The number of meals per day explained 10% (p < 0.01), and eating meals outside of the home accounted for 2.5% (p < 0.01) of the variation in GL; however, these results disappeared when adjusted for total energy intake. The GI was positively correlated with body mass index in girls (r = 0.05, p = 0.02), and GL was significantly higher among nonoverweight boys than overweight boys. This study identified eating patterns related to daily GI and GL, and suggests certain dietary patterns that could have beneficial effects on health. It also showed that GI and GL were weakly related to weight status.
The effect of a carbohydrate–caffeine sports drink on simulated golf performance
Authors: Stevenson Emma J. , Hayes Philip R. , Allison Sarah J. 
Published: September 2009

A round of golf lasts approximately 4 h, during which time homeostasis could be challenged through either hypoglycemia or hypohydration. This might result in impaired motor skill or cognitive performance. Given the high cognitive demand of putting and the potential fatiguing effects from prolonged walking, the combination of a caffeine and carbohydrate drink could be beneficial in offsetting hypoglycemia and hypohydration. This study used a laboratory-simulated round of golf to examine the effect of an isotonic carbohydrate and caffeine sports drink on putting performance during a round of golf. After institutional ethics approval, 20 male golfers (mean ± standard deviation: age 23 ± 4 years, stature 176.4 ± 5.6 cm, mass 72.8 ± 17.4 kg, handicap 15 ± 4, daily caffeine consumption 157.3 ± 47.2 mg) consumed either an isotonic sports drink containing caffeine (6.4 g carbohydrate and 16 mg caffeine per 100 mL) or a no-energy, flavour-matched placebo drink in a double-blind, randomized, counter-balanced crossover design . Drinks were consumed preround (5 mL·kg–1 body mass (BM)) and at holes 6 and 12 (2.5 mL·kg–1 BM). Participants therefore consumed 1.6 mg·kg–1 BM of caffeine and 0.64 g·kg–1 BM of carbohydrate throughout the trial. Five and 2 m putting performance were assessed at each hole. Self-rated mood assessments were carried out every third hole. Putting performance over 5 m and 2 m and self-rated scores for alertness and relaxation showed a main effect for drink (p < 0.05). Ratings of mental fatigue and tiredness significantly increased during the round (p < 0.001). In experienced golfers, the consumption of an isotonic carbohydrate sports drink containing caffeine prior to and during a round of golf improved putting performance and increased feelings of alertness.
Are Canadians meeting the guidelines for moderate and vigorous leisure-time physical activity?
Authors: Bryan Shirley N. , Katzmarzyk Peter T. 
Published: September 2009

The purpose of this study was to determine the proportion of Canadian adults (aged 18–55 years) who met the guidelines for moderate and vigorous physical activity set out in Canada’s Physical Activity Guide to Healthy Active Living. Leisure-time physical activity energy expenditure from moderate- and vigorous-intensity activities was calculated using data from the National Population Health Surveys (1994–1998) and the Canadian Community Health Surveys (2001–2007). The prevalence was estimated for no leisure-time physical activity, meeting only the moderate guideline, meeting both the moderate and vigorous guidelines, and meeting the guidelines through a combination of moderate and vigorous activities. Logistic regression was used to determine the odds of meeting the guidelines by various demographic characteristics. The prevalence of no activity did not change appreciably over time, ranging from 6.5% to 10%, depending on the survey year. Reporting of no activity was more prevalent among older adults, those in lower income groups, and those with a body mass index (BMI) ≥30 kg·m–2. Overall, 65% of adults met the guidelines for physical activity in 2007, which has increased from 54% in 1994–1995. Men, younger adults, those with a higher income, and those with a lower BMI more often met the guidelines. Among all subgroups, meeting the guidelines was most often accomplished through participation in moderate-intensity activities. These findings should be considered when designing and implementing public health interventions that promote participation in daily physical activity.
Antioxidant status, oxidative stress, and damage in elite kayakers after 1 year of training and competition in 2 seasons
Authors: Teixeira Vitor , Valente Hugo , Casal Susana , Pereira Laura , Marques Franklim , Moreira Pedro 
Published: September 2009

The large volume of training performed by elite athletes throughout the season can translate into a chronic oxidative insult. To study the effects that chronically high training loads have on athletes’ redox status, superoxide dismutase (SOD), glutathione reductase, glutathione peroxidase (GPx), and creatine kinase activities; total antioxidant status (TAS); and uric acid, retinol, α-tocopherol, α-carotene, β-carotene, lycopene, lutein + zeaxanthin, vitamin C, thiobarbituric acid reactive substances (TBARS), interleukin-6, and cortisol levels were determined in 9 kayakers (6 men) in a competitive period during the first season (June, T1), and in precompetitive (March, T2) and competitive (June, T3) periods during the following season. TAS decreased from the first to the second season (T1 vs. T2, p < 0.001; T1 vs. T3, p < 0.001). TBARS (p = 0.024) decreased from T1 to T2. The α-tocopherol increase (p = 0.001) from T1 to T2 lost statistical significance after adjustment for total lipids (p = 0.243). GPx (p = 0.003) increased, while SOD (p < 0.001) and uric acid (p = 0.032) decreased from T2 to T3. Cortisol levels decreased significantly throughout the study (T1 vs. T2, p = 0.042; T2 vs. T3, p = 0.018; T1 vs. T3, p = 0.002). No significant differences were observed for any of the other parameters studied. Antioxidant status changed more within the same season than from one season to another. Redox markers should be monitored throughout the season to detect athletes at an increased oxidative risk.