bluuebunny/crossref_metadata_2025_split huggingface dataset:
Silica nanoparticles-induced cytotoxicity and genotoxicity in A549 cell lines
Authors: Peivandi Zahra , Shirazi Farshad H. , Teimourian Shahram , Farnam Golrokh , Babaei Vahid , Mehrparvar Neda , Koohsari Nasim , Ashtarinezhad Azadeh 
Published: June 2024

AbstractAmong the myriad of nanoparticles, silica nanoparticles (SiO2NPs) have gained significant attention since they are extensively produced and used across several kinds of industries. Because of its widespread usage, there has been increasing concern about the potential health effects. This study aims to evaluate the effects of SiO2NPs on Interleukin-6 (IL-6) gene expression in human lung epithelial cell lines (A549). In this study, A549 cells were exposed to SiO2NPs at concentrations of 0, 1, 10, 50, 100, and 200 µg/mL for 24 and 48 h. The IL-6 gene expression was assessed using Real-Time RT-PCR. Additionally, the impact of SiO2NPs on the viability of A549 cells was determined by MTT assay. Statistical analysis was performed using GraphPad Prism software 8.0. MTT assay results indicated a concentration-dependent impact on cell survival. After 24 h, survival decreased from 80 to 68% (1–100 µg/mL), rising to 77% at higher concentrations. After 48 h, survival dropped from 97 to 80%, decreasing to 90% at higher concentrations. RT-PCR showed a dose–response relationship in cellular toxicity up to 10 µg/mL. At higher concentrations, there was increased IL-6 gene expression, mitigating SiO2NP-induced cytotoxic effects. The study shows that the viability and proliferation of A549 cells are impacted by different SiO2NPs concentrations. There may be a potential correlation between IL-6 gene expression reduction and a mechanism linked to cellular toxicity. However, at higher concentrations, an unknown mechanism increases IL-6 gene expression, reducing SiO2NPs' cytotoxic effects. These effects are concentration-dependent and not influenced by exposure times. Further investigation is recommended to determine this mechanism's nature and implications, particularly in cancer research.
Mechanism of oxymatrine in the treatment of cryptosporidiosis through TNF/NF-κB signaling pathway based on network pharmacology and experimental validation
Authors: Zhang Xiaoning , Shi Jie , Lu Yilong , Ji Rui , Guan Zhiyu , Peng Fujun , Zhao Chunzhen , Gao Wei , Gao Feng 
Published: June 2024

AbstractCryptosporidiosis is a worldwide zoonotic disease. Oxymatrine, an alkaloid extracted and isolated from the plant bitter ginseng, has been reported to have therapeutic effects on cryptosporidiosis. However, the underlying mechanism of its action remains unclear. In this study, we utilized network pharmacology and experimental validation to investigate the mechanism of oxymatrine in the treatment of cryptosporidiosis. First, the potential targets of drugs and diseases were predicted by TCMSP, Gene Cards, and other databases. Following the intersection of drug-disease targets, the DAVID database was used to implement the enrichment analysis of GO functions and KEGG pathways, and then the network diagram of "intersected target-KEGG" relationship was constructed. Autodock 4.2.6 software was used to carry out the molecular docking of core targets to drug components. Based on the establishment of a mouse model of cryptosporidiosis, the validity of the targets in the TNF/NF-κB signaling pathway was confirmed using Western blot analysis and Quantitative Rea-ltime-PCR. A total of 41 intersectional targets of oxymatrine and Cryptosporidium were generated from the results, and five core targets were screened out by network analysis, including RELA, AKT1, ESR1, TNF, and CASP3. The enrichment analysis showed that oxymatrine could regulate multiple gene targets, mediate TNF, Apoptpsis, IL-17, NF-κB and other signaling pathways. Molecular docking experiments revealed that oxymatrine was tightly bound to core targets with stable conformation. Furthermore, we found through animal experiments that oxymatrine could regulate the mRNA and protein expression of IL-6, NF-κB, and TNF-α in the intestinal tissues of post-infected mice through the TNF/NF-κB signaling pathway. Therefore, it can be concluded that oxymatrine can regulate the inflammatory factors TNF-α, NF-κB, and IL-6 through the TNF/NF-κB signaling pathway for the treatment of cryptosporidiosis. This prediction has also been validated by network pharmacology and animal experiments.
Virulence and resistance profiling of Staphylococcus aureus isolated from subclinical bovine mastitis in the Pakistani Pothohar region
Authors: Shahzad Muhammad Armaghan , Yousaf Arfan , Ahsan Aitezaz , Irshad Hamid , Riaz Aayesha , Khan Asghar , Ullah Inayat , Sattar Sadia , Bostan Nazish , Javed Sundus 
Published: June 2024

AbstractMastitis is considered one of the most widespread infectious disease of cattle and buffaloes, affecting dairy herds. The current study aimed to characterize the Staphylococcus aureus isolates recovered from subclinical mastitis animals in Pothohar region of the country. A total of 278 milk samples from 17 different dairy farms around two districts of the Pothohar region, Islamabad and Rawalpindi, were collected and screened for sub clinical mastitis using California Mastitis Test. Positive milk samples were processed for isolation of Staphylococcus aureus using mannitol salt agar. The recovered isolates were analyzed for their antimicrobial susceptibility and virulence genes using disc diffusion and PCR respectively. 62.2% samples were positive for subclinical mastitis and in total 70 Staphylococcus aureus isolates were recovered. 21% of these isolates were determined to be methicillin resistant, carrying the mecA gene. S. aureus isolates recovered during the study were resistant to all first line therapeutic antibiotics and in total 52% isolates were multidrug resistant. SCCmec typing revealed MRSA SCCmec types IV and V, indicating potential community-acquired MRSA (CA-MRSA) transmission. Virulence profiling revealed high prevalence of key genes associated with adhesion, toxin production, and immune evasion, such as hla, hlb, clfA, clfB and cap5. Furthermore, the Panton-Valentine leukocidin (PVL) toxin, that is often associated with recurrent skin and soft tissue infections, was present in 5.7% of isolates. In conclusion, the increased prevalence of MRSA in bovine mastitis is highlighted by this study, which also reveals a variety of virulence factors in S. aureus and emphasizes the significance of appropriate antibiotic therapy in combating this economically burdensome disease.
A microscopic mechanism study of the effect of binary surfactants on the flotation of Wiser bituminous coal
Authors: Zhang Chun , Qian Xianju , Song Hailong , Jia Jinzhang 
Published: June 2024

AbstractInvestigating surfactant effects on the floatability of Wiser bituminous coal holds significant importance in improving coal cleanliness and utilization value. Using density functional theory and molecular dynamics simulation methods, this study constructed models of Wiser bituminous coal and examined the impact of different surfactants, including the anionic surfactant sodium dodecyl benzene sulfonate, the cationic surfactant hexadecyl trimethyl ammonium bromide (CTAB), and the non-ionic surfactant fatty alcohol ethoxylated ether. The focus was on investigating the charge distribution characteristics of these molecules and the modifying effect of binary surfactants on the hydrophobicity of bituminous coal. Results revealed that the maximum electrostatic potential was concentrated near oxygen/nitrogen/sulfur-containing functional groups like sulfonic acid groups, quaternary ammonium cations, ethylene oxide, hydroxyl groups, carboxyl groups, and sulfur bonds. These functional groups exhibited a propensity for accepting/delivering electrons to form hydrogen bonds. Among the surfactants tested, CTAB revealed the slightest difference in frontier orbital energy, measuring 3.187 eV, thereby demonstrating a superior trapping ability compared with the other two surfactants. Adsorption reactions within the system were determined to be spontaneous, with over 60% of the interaction force attributed to electrostatic forces. Moreover, the repulsive force magnitude with water molecules followed the trend: sulfonate group (2.20 Å) < ethylene oxide (2.43 Å) < quaternary ammonium cation (2.57 Å), indicating more excellent water repellency of CTAB. Findings showed that CTAE binary surfactants proved most effective in modifying the hydrophobicity of bituminous coal. This study offers valuable insights into reducing waste, pollution, and resource wastage.
First-line serplulimab plus chemotherapy versus chemotherapy in PD-L1-positive esophageal squamous-cell carcinoma: a cost-effectiveness analysis
Authors: Liang Xueyan , Meng Mingyu , Qin Shiran , Chen Xiaoyu , Li Yan 
Published: June 2024

AbstractPatients with PD-L1-positive esophageal squamous-cell carcinoma (ESCC) were significantly more likely to survive when treated with serplulimab plus cisplatin plus 5-fluorouracil (serplulimab-CF). At this point, it is unknown whether this expensive therapy is cost-effective. From the Chinese healthcare system's perspective, we aimed to evaluate serplulimab-CF versus CF alone for cost-effectiveness. A partitioned survival model was constructed based on the ASTRUM-007 trial. Quality-adjusted life-years (QALYs) and incremental cost-effectiveness ratio (ICER) were calculated. A further analysis of subgroups and scenarios was conducted. The willingness to pay (WTP) threshold of $38,258/QALY or $84,866/QALY is defined as three times the per capita gross domestic product value of the general region or affluent region. Compared with CF alone, in the overall (scenario 1), patients with PD-L1 expression level of 1 ≤ CPS < 10 (scenario 2), and patients with PD-L1 CPS ≥ 10 (scenario 3) populations, the ICERs were $69,025/QALY, $82,533/QALY, and $75,436/QALY for serplulimab-CF. Nevertheless, the probability of serplulimab-CF becoming cost-effective based on scenarios 1, 2, and 3 is only 2.71%, 0.94%, and 2.84%, respectively, at a WTP threshold of $38,258/QALY. When serplulimab costs < $4.84/mg, serplulimab-CF may be cost-effective at the WTP threshold of $38,258/QALY; otherwise, CF was preferred. Similar results were obtained from sensitivity analyses, suggesting the robustness of these findings. There was no cost-effectiveness in general regions of China for serplulimab-CF in PD-L1-positive ESCC compared to CF, although it is probably considered cost-effective in affluent regions. Serplulimab-CF may achieve favorable cost-effectiveness by lowering the price of serplulimab.
Kinetic nitrogen isotope effects of 18 amino acids degradation during burning processes
Authors: Zhu Ren-Guo , Xiao Hua-Yun , Yin Meiju , Xiao Hao , Zhou Zhongkui , Wei Guo , Liu Cheng , Hu Caixia 
Published: June 2024

AbstractUnderstanding the nitrogen isotopic variations of individual amino acids (AAs) is essential for utilizing the nitrogen isotope values of individual amino acids (δ15N-AA) as source indicators to identify proteinaceous matter originating from biomass combustion processes. However, the nitrogen isotope effects (ε) associated with the degradation of individual amino acids during combustion processes have not been previously explored. In this study, we measured the nitrogen isotope values of residual free amino acids -following a series of controlled combustion experiments at temperatures of 160–240 °C and durations of 2 min to 8 h, as described in Part 1. δ15N values of proline, aspartate, alanine, valine, glycine, leucine, and isoleucine are more positive than their initial δ15N values after prolonged combustion. Variations in δ15N values of the most AAs conform to the Rayleigh fractionation during combustion and their nitrogen isotope effects (ε) are greatly impacted by their respective combustion degradation pathways. This is the first time the ε values associated with the degradation pathways of AAs during combustion have been characterized. Only the ε values associated with Pathway 1 (dehydration to form dipeptide) and 2 (simultaneous deamination and decarboxylation) are found to be significant and temperature-dependent, ranging from + 2.9 to 6.4‰ and + 0.9‰ to + 3.8‰, respectively. Conversely, ε values associated with other pathways are minor. This improves the current understanding on the degradation mechanisms of protein nitrogen during biomass burning.
The substitute ENSO 16 has low impact on glucose metabolism in healthy humans: a randomized, double-blind, active-controlled, cross-over trial
Authors: Lutnik Martin , Weisshaar Stefan , Mussbacher Lena M. , Steiner Daniel , Wolzt Michael 
Published: June 2024

AbstractHigh sugar consumption is associated with cardiovascular diseases and diabetes. Current sugar substitutes may cause taste sensations and gastrointestinal symptoms. ENSO 16 is a combination of 16 different sugar substitutes and plant fibers and has been designed as a sugar alternative. The impact on plasma glucose metabolism as well as on gastrointestinal tolerance has not been investigated yet. 17 healthy participants were enrolled in this randomized, double-blind trial. Participants received a single oral dose of 30 g glucose or 30 g ENSO 16 and crossed over to the alternate treatment after a 7 day wash out period. The study endpoint was the effect on plasma glucose, insulin, C-peptide concentrations and gastrointestinal disorders. A questionnaire regarding gastrointestinal symptoms was used for individual subjective scoring. The mean baseline adjusted plasma glucose AUC0–180 min was significantly greater after glucose administration compared to ENSO 16 (n = 15, p = 0.0128, paired t-test). Maximum plasma glucose elevation over baseline was 117 mg*dl−1 and 20 mg*dl−1 after oral glucose or ENSO 16, respectively. Insulin and C-peptide AUC0−180 min were significantly greater after glucose compared to ENSO 16 intake (p < 0.01, Wilcoxon rank sum test). The mean maximal concentrations of plasma glucose, insulin and C-peptide after glucose intake were 1.5, 4.6 and 2.7-fold greater after glucose intake compared to ENSO 16 intake, respectively. Adverse reactions were mostly mild and not different between treatments. Conclusion. ENSO 16 has only a small impact on plasma glucose metabolism. This may be of interest in a dietary context and may help to reduce calory intake.Trail registration NCT05457400. First registration: 14/07/2022. https://clinicaltrials.gov/study/NCT05457400.
Boundary quantitative characterization of the top-coal limit equilibrium zone in fully mechanized top-coal caving stope along the strike direction of working face
Authors: Lang Ding , Wu Xiaobo , Wu Yongping , Xie Panshi , Yan Zhuangzhuang , Chen Shuaiming 
Published: June 2024

AbstractIn the process of fully mechanized top-coal caving mining, the top-coal is affected by mining-induced stress, and the stress varies along the strike direction of working face, so the boundary position of its entering the limit equilibrium state changes accordingly. The determination of the boundary along the strike direction of working face can provide scientific guidance for the stability control of support-surrounding rock in fully mechanized top-coal caving face. Using the research methods of theoretical analysis, physical similarity simulation experiment and numerical simulation experiment, the stress state analysis model of the boundary position of the top-coal limit equilibrium zone under macro-scale conditions was established, the stress state characterization method of the boundary of the top-coal limit equilibrium zone along the strike direction of working face was given, and the quantitative characterization of the boundary of the top-coal limit equilibrium zone along the strike direction of working face was realized by combining with the mining-induced stress path, and the distance relationship between the boundary of the top-coal limit equilibrium zone and the langwall face along the strike direction of working face was revealed. The results show that after critical mining in fully mechanized top-coal caving face, the distance between the boundary of top-coal limit equilibrium zone and the langwall face along the strike direction of working face presents a relationship of increasing from top to bottom. The distance between the top-coal upper boundary and the langwall face was 2.85 m and the distance between the top-coal lower boundary and the langwall face was 5.39 m. The boundary of top-coal limit equilibrium zone along the strike direction of working face was verified by the top-coal elastic–plastic zone boundary and the boundary of the peak position of front abutment pressure in different layers of top-coal. The results show that the quantitative characterization of the top-coal limit equilibrium zone boundary along the strike direction of working face was reasonable. In order to improve mine production efficiency, optimization measures were put forward for hard coal seam and soft coal seam respectively.
Biomechanical analysis of trunk and lower limbs during stair activity in patients with scoliosis
Authors: Gou Yanyun , Tao Jing , Huang Jia , Lei Huangwei , Chen Xiang , Wang Xiangbin 
Published: June 2024

AbstractStaircases are a frequently encountered obstacle in daily life, requiring individuals to navigate ascending and descending movements that place additional demands on the trunk and lower limbs compared to walking on level surfaces. Therefore, it is crucial to examine the biomechanical characteristics of the trunk and lower limbs in individuals with scoliosis during stair activity. The aim of this study was to investigate the biomechanical differences in trunk and lower limbs during daily stair activities between patients with scoliosis and a healthy population. Additionally, the study aimed to explore the relationship between trunk abnormalities and lower limb biomechanics, providing a clinical and objective assessment basis for scoliosis. The Qualisys system, based in Gothenburg, Sweden, was employed for data collection in this study, with a sampling frequency of 150 Hz. It captured the kinematics of the trunk and lower limbs, as well as the kinetics of the lower limbs during stair ascent and descent for both the 28 individuals with scoliosis and the 28 control participants. The results indicate that scoliosis patients demonstrated significantly higher asymmetry compared to the control group in various measures during ascent and decent. These include different parts of kinematics and kinetics. Scoliosis patients demonstrate noticeable variations in their movement patterns compared to the healthy population when engaging in stair activities. Specifically, during stair ascent, scoliosis patients exhibit a seemingly more rigid movement pattern, whereas descent is characterized by an unstable pattern.
A demonstration trajectory segmentation approach for wheelchair‐mounted assistive robots
Authors: Chi Mingshan , Liu Yaxin , Zhang Qiang , Zeng Chao 
Published: June 2024

AbstractSegmentation of demonstration trajectories and learning the contained motion primitives can effectively enhance the assistive robot's intelligence to flexibly reproduce learnt tasks in an unstructured environment. With the aim to conveniently and accurately segment demonstration trajectories, a novel demonstration trajectory segmentation approach is proposed based on the beta process autoregressive hidden Markov model (BP‐AR‐HMM) algorithm and generalised time warping (GTW) algorithm aiming to enhance the segmentation accuracy utilising acquired demonstration data. This approach first adopts the GTW algorithm to align the multiple demonstration trajectories for the same task. Then, it adopts the BP‐AR‐HMM algorithm to segment the demonstration trajectories, acquire the contained motion primitives, and establish the related task library. This segmentation approach is validated on the 6‐degree‐of‐freedom JACO robotic arm by assisting users to accomplish a holding water glass task and an eating task. The experimental results show that the motion primitives within the trajectories can be correctly segmented with a high segmentation accuracy.
Finite‐time control of DC–AC boost converter: The limit cycle approach
Authors: Azhdari Meysam , Binazadeh Tahereh 
Published: June 2024

AbstractThis paper proposes a novel finite‐time limit cycle controller for DC to AC conversion in power boost converters. The stable limit cycle is categorized as a positive limit set. Achieving the control law, based on the concepts of set stability and finite‐time stability, is a challenging problem. To fulfill the control objective, a novel sliding manifold is suggested concerning the structure of the wanted limit cycle which facilitates conditions for the development of finite‐time stability for the limit cycle. By utilizing the sliding mode control method, a control strategy is proposed that guarantees the desired stable limit cycle is created in the phase plane of the closed‐loop system and the phase trajectories reach it in a finite time and remain on it for all future times. This ensures the finite‐time generation of the biased sinusoidal oscillations in the output of the power boost converter from a DC input source. The simulation results of a practical boost converter have validated the effectiveness and feasibility of the presented algorithm.
Evidenz der modernen Diagnostik, der konservativen und chirurgischen
Therapie des sekundären Lymphödems
Authors: Will Patrick , Dragu Adrian , Zuther Joachim , Heil Jörg , Chang De-Hua , Traber Jürg , Hirche Christoph 
Published: June 2024

Zusammenfassung
Hintergrund Das sekundäre Lymphödem (SL) stellt eine der häufigsten und
zugleich bedeutsamsten Folgen und Komplikation der modernen onkologischen
Therapie dar. Obwohl eine gründliche Anamnese und körperlicher Untersuchung für
den Verdacht ausreichen, ist eine funktionelle Bildgebung des Lymphsystems für
die gezielte Diagnose und Ermittlung des Schweregrads essenziell. Hierfür wurden
in den letzten Jahren Techniken wie die MR-und die ICG-Lymphographie sowie die
ultrahochfrequenten Ultraschalluntersuchungen entwickelt und validiert. Die
selektive Anwendung dieser Techniken hat die individualisierte
Indikationsstellung und erfolgreiche stadienabhängige Behandlung mit den
(super-) mikrochirurgischen Techniken zur Wiederherstellung des regionalen
Lymphabflusses im Kontext einer intensivierten konservativen Therapie erst
möglich gemacht.
Methode Systematischer Review der Literatur zur Diagnose und Therapie des
SL mit anschließender Analyse und Klassifikation der Ergebnisse in Evidenzlevel
gemäß Oxford Centre for Evidence-Based Medicine sowie gemäß der GRADE Skala.
Ergebnisse Zur etablierten und validierten Diagnostik des SL gehören
Bildgebungen (ICG Fluoreszenzlymphographie, MR-Lymphographie und Tc-99
Funktionslymphszintigraphie) bei klinischem Verdacht und Hochrisikopatienten.
Komplexe physikalische Entstauungstherapie (KPE) ist der Physiotherapie oder
Kompression allein überlegen. Die (Super-)Mikrochirurgie des SL ermöglicht eine
postoperative Reduktion der Frequenz der KPE, Reduktion von Erysipelraten,
Volumenreduktion der lymphomatösen Extremität und bei prophylaktischer
Durchführung eine geringere Inzidenz des SL. Saugungsverfahren können
langfristig stabile Umfangsreduktionen und eine Verbesserung der Lebensqualität
erzeugen.
Schlussfolgerung Patienten mit SL profitieren von einer konservativen
Therapie mit regelmäßiger Reevaluation. Hochrisikopatienten für ein SL oder
Patienten mit einer klinischen Verschlechterung oder gleichbleibendem
Leidensdruck unter leitliniengerechter konservativer Therapie können von
(super-)mikrochirurgischen Therapien nach individualisierter Funktionsdiagnostik
des Lymphsystems profitieren. Exzisionale Dermolipektomien oder
Lympholiposuktionen sind für fortgeschrittene und therapierefraktäre Stadien
verfügbar und wirksam.