bluuebunny/crossref_metadata_2025_split huggingface dataset:
WITHDRAWN: Suicide or self-preservation：C2H2 Zinc Finger Protein ZNF775 inhibits MCF-7 breast cancer cell migration by downregulating Wnt5a
Authors: Zhu Xin , Gong Wei , Zhang Wenwu , Song Xiaoyu , Hu Junjie , Xu Weihua , Ma Zhichao , Xiao Bin , Li Linhai , Chen Xinping 
Published: April 2024

Abstract

C2H2 zinc finger protein is widely involved in the occurrence and development of cancer. However, the function and mechanism of most C2H2 zinc finger proteins in breast caner (BC) remains unclear. Here, we reported the expression prognosis of C2H2 type zinc finger protein ZNF775 in BC patients and its possible biological mechanism. First, multiple public databases showed that ZNF775 was significantly overexpressed in BC tissues and cells. Interestingly, high expression of ZNF775 was significantly associated with a better prognosis. Western blot and immunohistochemistry were used for verification, and the expression of ZNF775 was consistent with the databases. In vitro overexpression experiments showed that overexpression of ZNF775 significantly inhibited the proliferation and migration of MCF-7 BC cell. We further combined RNA-sequencing (RNA-seq) and CUT & Tag, and found that overexpression of ZNF775 can down-regulate the expression of most genes in the Wnt signaling pathway. The cBioportal database showed that ZNF775 was negatively correlated with the expression of Wnt5a, suggesting that its downstream target was likely Wnt5a. Finally, we discovered that Wnt5a could partially reverse the inhibitory effect of ZNF775 on MCF-7 BC cell migration through transwell migration experiments. In conclusion, our findings will provide new ideas for the diagnosis, treatment and prognosis assessment of BC in the future.
Persistent Impact of Antenatal Maternal Anaemia on Child Brain Structure at 6-7 Years of Age: A South African Child Health Study
Authors: Ringshaw Jessica E. , Hendrikse Chanelle , Wedderburn Catherine J. , Bradford Layla E. , Williams Simone R. , Nyakonda Charmaine N. , Subramoney Sivenesi , Lake Marilyn T. , Burd Tiffany , Hoffman Nadia , Roos Annerine , Narr Katherine L. , Joshi Shantanu H. , Williams Steven C.R. , Zar Heather J. , Stein Dan J. , Donald Kirsten A. 
Published: April 2024

Abstract

Background
The study aim was to determine whether associations of antenatal maternal anaemia with smaller corpus callosum, putamen, and caudate nucleus volumes previously described in children at age 2–3 years persist to age 6-7 years in the Drakenstein Child Health Study (DCHS).
Methods
This neuroimaging sub-study was nested within the DCHS, a South African population-based birth cohort. Pregnant women were enrolled (2012-2015) and mother-child dyads were followed prospectively. A sub-group of children had magnetic resonance imaging at 6-7 years of age (2018-2022). Mothers had haemoglobin measurements during pregnancy and a proportion of children were tested postnatally. Maternal anaemia (haemoglobin<11g/dL) and child anaemia were classified using WHO and local guidelines. Linear modeling was used to investigate associations between antenatal maternal anaemia status, maternal haemoglobin concentrations, and regional child brain volumes. Models included potential confounders and were conducted with and without child anaemia to assess the relative roles of antenatal versus postnatal anaemia.
Results
Overall, 157 children (Mean [SD] age of 75.54 [4.77] months; 84 [53.50%] male) were born to mothers with antenatal haemoglobin data. The prevalence of maternal anaemia during pregnancy was 31.85% (50/157). In adjusted models, maternal anaemia status was associated with smaller volumes of the total corpus callosum (adjusted percentage difference, -6.77%; p=0.003), left caudate nucleus (adjusted percentage difference, -5.98%, p=0.005), and right caudate nucleus (adjusted percentage difference, -6.12%; p=0.003). Continuous maternal haemoglobin was positively associated with total corpus callosum (β=0.239 [CI: 0.10 to 0.38]; p<0.001) and caudate nucleus (β=0.165 [CI: 0.02 to 0.31]; p=0.027) volumes. In a sub-group (n=89) with child haemoglobin data (Mean [SD] age of 76.06[4.84]), the prevalence of antenatal maternal anaemia and postnatal child anaemia was 38.20% (34/89) and 47.19% (42/89), respectively. There was no association between maternal and child anaemia (c2 = 0.799; p=0.372), and child anaemia did not contribute to regional brain volume differences associated with maternal anaemia.
Conclusions
Associations between maternal anaemia and regional child brain volumes previously reported at 2-3 years of age were consistent and persisted to 6-7 years of age. Findings support the importance of optimizing antenatal maternal health and reinforce these brain regions as a future research focus on intervention outcomes.
Hybrid motility mechanism of sperm at viscoelastic-solid interface
Authors: Unnikrishnan Shobitha , Scott Robert , Ogundele Emmanuel , Azad Mohammad , Ishimoto Kenta , Suarez Susan , Tung Chih Kuan 
Published: April 2024

Abstract

To fertilize eggs, sperm must pass through narrow, complex channels filled with viscoelastic fluids in the female reproductive tract. While it is known that the topography of the surfaces plays a role in guiding sperm movement, sperm have been thought of as swimmers, i.e., their motility comes solely from sperm interaction with the surrounding fluid, and therefore, the surfaces have no direct role in the motility mechanism itself. Here, we examined the role of solid surfaces in the movement of sperm in a highly viscoelastic medium. By visualizing the flagellum interaction with surfaces in a microfluidic device, we found that the flagellum stays close to the surface while the kinetic friction between the flagellum and the surface is in the direction of sperm movement, providing thrust. Additionally, the flow field generated by sperm suggests slippage between the viscoelastic fluid and the solid surface, deviating from the no-slip boundary typically used in standard fluid dynamics models. These observations point to hybrid motility mechanisms in sperm involving direct flagellum-surface interaction in addition to flagellum pushing the fluid. This finding signifies an evolutionary strategy of mammalian sperm crucial for their efficient migration through narrow, mucus-filled passages of the female reproductive tract.
Real-world data to improve Organ and Tissue Donation Policies: Lessons learned from the Tissue and Organ Donor Epidemiology Study
Authors: Greenwald Melissa A. , Ezzeldin Hussein , Blumberg Emily A. , Whitaker Barbee I , Forshee Richard A 
Published: April 2024

Abstract

Background: The transplantation of human organs, and some human tissues, is often the only life-saving therapy available for serious and life-threatening congenital, inherited, or acquired diseases. However, it is associated with a risk of transmission of communicable diseases from donor to recipient. It is imperative to understand the characteristics of the donor population to inform policies that protect recipient safety. The Tissue and Organ Donor Epidemiology Study (TODES) was a pilot project designed to identify and collect standardized information on deceased persons referred for organ, tissue, and/or eye donation, and to estimate (to the extent possible) infectious disease prevalence and incidence of HIV, HBV, and/or HCV in this population. TODES is summarized here to shed light on addressable limitations on accessing data needed for transplant recipient safety. Limitations, future research needs, and potential pathways to solve the remaining data needs are explored.
Methods: Retrospective data for all deceased donors during a 5-year period from 2009 to 2013 were obtained from participating organ procurement organizations (OPOs), tissue establishments, and eye banks. These decedent data on actual donors and potential donors were used to ascertain whether the available real-world data (RWD) could be used to inform donor screening and testing policy.
Results: The TODES database contains 291,848 records received from nine OPOs and 42,451 records received from four eye banks. Data were analyzed from deceased donors with at least one organ, tissue, or ocular tissue recovered with the intent to transplant. Results for potential donors were not analyzed. Available RWD at the time of the TODES study were not fit-for-purpose to help characterize the organ- and tissue eye donor populations and/or to inform donor screening policy.
Conclusions: Recent advances in electronic data collection systems make it more realistic to now collect fit-for-purpose RWD that address the research needed to improve transplant safety.
Unsupervised feature selection based on the hidden knowledge of the Two-Dimensional Principal Component Analysis feature extraction method
Authors: Beiranvand Firoozeh , Mehrdad Vahid , Dowlatshahi Mohammad Bagher 
Published: April 2024

Abstract

In this paper, we proposed a new matrix-based feature selection method that used the hidden knowledge in the orthogonal features obtained from the two-dimensional principal component analysis feature extraction method with transfer learning to perform highly accurate unsupervised feature selection. We briefly named it the UFS2DPCA algorithm. In general, features can be classified as redundant, irrelevant, and relevant. Correlation is another concept of redundancy and perfectly correlated features are redundant. Accordingly, we first use the 2DPCA approach to directly extract the uncorrelated and orthogonal features from the 2-D image dataset. Uncorrelated and orthogonal features are among the best features. Next, we compute the correlation similarity between the main and extracted features. Finally, we make a weighted bipartite graph using two sets of features and the similarities between them, then we select the best features of the primary using the fast LAPJV algorithm. We evaluate the performance of the proposed UFS2DPCA algorithm on four well-known image datasets using K- Nearest Neighbor classifier. Results of comparative experiments between the proposed UFS2DPCA algorithm and eight state-of-the-art unsupervised feature selection algorithms show that the UFS2DPCA method outperforms other methods.
Class A1 scavenger receptor mediates macrophage polarization and gut microbial alteration in a murine dextran sulfate sodium-induced colitis
Authors: Su Jingling , Liu Lupeng , Ren Yandan , Gan Yutong , Lin Yumei , Xie Chenxi 
Published: April 2024

Abstract

This study aimed to investigate whether class A1 scavenger receptor (SR-A1) regulated macrophage polarization and gut microbial alteration during intestinal inflammation of colitis. A murine colitis model was established by feeding with dextran sulfate sodium (DSS), and treatment groups were injected intravenously with SR-A1 antibody. Results showed a preventive effect on colitis symptoms and fewer inflammatory cell infiltrates in treatment groups. Down-regulation of inflammatory cytokines and up-regulation of anti-inflammatory cytokine related to macrophages were seen in murine PBMC and LPMC after injected with SR-A1 antibody. The percentage of M2 macrophages was also elevated in treatment groups. In addition, SR-A1 antibody treatment resulted in the decreased apoptosis and increased proliferation of colonic epithelial cells. Other findings indicated that SR-A1 injection could mediate its anti-inflammatory effect via inhibiting TLR4-MyD88-NF-kB signaling pathway and alterating the gut microbiota composition. Our research identified SR-A1 as a potential therapeutic target in inflammatory bowel disease (IBD).
Advancement of the Healthcare Economy in Iran
Authors: monfared zeinab 
Published: April 2024

Abstract

Background:
Iran's healthcare economy has undergone significant advancements in recent years, driven by various reforms and investments. Understanding the trajectory of this progress is essential for policymakers and stakeholders to ensure continued growth and improvement in healthcare delivery and outcomes.
Objective:
This study aims to assess the advancement of the healthcare economy in Iran, highlighting achievements, challenges, and areas for further development.
Methods:
A mixed-methods approach was employed, incorporating both qualitative and quantitative techniques. Data from government reports, academic publications, and expert interviews were analyzed to provide a comprehensive overview of Iran's healthcare system evolution, infrastructure, service accessibility, and technological advancements.
Results:
The analysis revealed notable progress in Iran's healthcare economy, including improvements in infrastructure, expanded access to services, and adoption of advanced medical technologies. However, challenges persist, particularly in healthcare financing and workforce capacity, hindering optimal healthcare delivery.
Conclusions:
The findings suggest that while Iran has made significant strides in advancing its healthcare economy, there is a need for continued investment and strategic planning to address existing challenges and sustain progress. Policymakers should prioritize initiatives aimed at enhancing healthcare financing mechanisms, strengthening workforce capacity, and optimizing resource allocation to ensure equitable access to quality healthcare services for all citizens.
Medical students’ journey towards Cultural Humility – navigating diverse others and systems with extreme inequity
Authors: Botha Gertruida Catherina , Frambach Jannecke , Krumeich Anja , Mokgatle Mathildah 
Published: April 2024

Abstract

Introduction: Cultural humility (CH) is a vital journey for addressing diversity, but its application remains elusive. While existing literature covers cultural humility’s why and what mainly in the context of western countries, the holistic development of its parts during medical training remains underexplored. Given the foundational role of interactions in cultural humility, this study explores its development during various interactions with peers, teachers, patients and researchers across a diversity of education and clinical health care settings marked by inherent power imbalance and inequity.
Methods: An interpretivist qualitative case study approach was employed, involving purposive sampling of diverse medical students from one medical school in a country with one of the highest inequity coefficients in the world. Data collection was through semi-structured interviews and analysed using thematic analysis. Ethical clearance and participant consent was obtained.
Results: Interactions between actors unfolded in multiple dimensions and layers. Findings were classified into four themes i.e. journeying from feeling like an outsider to embracing interactions, from absolute truth to questioning perceptions, journeying within power imbalance; and embracing future roles through introspection. For participants two fundamental dilemmas remained, i.e. whether to navigate social relations and how to navigate intergroup conflict.
Discussion: This study argues that the development of CH is context based and dynamic; however, it’s development should not be assumed but should be considered as multifaceted and layered, where the individual process is significantly influenced by past contexts as well as enhancing interactions with peers, teachers, patients and researchers both formally and informally.
Clinical outcome of adjuvant chemotherapy for locally advanced head and neck squamous cell carcinoma
Authors: chen li , zhang tao 
Published: April 2024

Abstract

Objective: To explore the role of adjuvant chemotherapy in the treatment of locally advanced head and neck squamous cell carcinoma.
Methods: A retrospective analysis was performed on 360 newly diagnosed patients with head and neck squamous cell carcinoma. One group received adjuvant chemotherapy after radical treatment, and the other group did not receive adjuvant chemotherapy. Radical treatment included radical surgery and radical radiotherapy/chemoradiotherapy. Kaplan-Meier method was used to calculate overall survival (OS) and event-free survival (EFS), the Cox proportional risk model was used to study the risk factors affecting the prognosis of patients, and the patients were divided into radical radiotherapy group/chemoradiotherapy group, and radical surgery group (simple radical surgery, surgery + radiotherapy, surgery + concurrent chemoradiotherapy) according to whether the surgery was performed.
Results: Among 360 patients, the median survival time was （3138） days in the adjuvant chemotherapy group and （2212d） in the non-adjuvant chemotherapy group (P=0.02). In the radical radiotherapy/chemoradiotherapy group, the median survival time was （1974）days in the adjuvant chemotherapy group and（635）days in the non-adjuvant chemotherapy group, the difference was statistically significant (P=0.005). In the radical surgery group, adjuvant chemotherapy did not prolong patients' OS but had certain benefits in EFS. The median event-free survival in the adjuvant chemotherapy group versus the non-adjuvant chemotherapy group was （3138）days vs （2019）days, P=0.038.
Conclusion: Adjuvant chemotherapy may be valuable in patients with high-risk pathological factors after radical radiotherapy/chemoradiotherapy and radical surgery. Conclusions： Adjuvant chemotherapy may be valuable in patients with high-risk pathologic factors after radical surgery and after radical radiotherapy/chemoradiotherapy
RESTRICTION FRAGMENT LENGTH POLYMORPHISM IN CARROT
Authors: Sampath Vivek , Simon Philipp 
Published: April 2024

Studies of genetic variation at the DNA level in the genus Daucus have been very limited. Recently, molecular markers based on restriction fragment length polymorphism (RFLP) have been shown to be highly useful and efficient gene markers in other plant and animal species.We have used a total of 40 carrot strains (inbreds, varieties, species) for this study. Genomic DNA probes cloned in pGEM (Promega) plasmid of Escherichia coli were hybridized with genomic DNA of restriction enzymes. We have found RFLP variation to be extensive in Daucus, even among related cultivation genetic stocks. The implications of these results in the germplasm diversity in Daucus will be discussed.
MODIFICATION OF MECHANICAL STRESS EFFECTS ON ELONGATION OF DARK-GROWN SOYBEAN BY Ca2+ AND K+
Authors: Myers Patricia N. , Mitchell Cary A. 
Published: April 2024

A single episode of thigmic (contact rubbing) stress applied to the apical hook of a dark-grown soybean hypocotyl results in a two-phase response: an initial rapid (s) cessation of elongation followed by a long (h) recovery. Increased Ca2+ in the growth matrix from 1 to 5 mM increased the average pre-stress elongation rate from 22 μm min-1 to 40 μm min-1 further increases in Ca2+ did not further enhance elongation rate, There was no effect of nutritional status on the initial rapid response to a single episode of thigmic stress; all seedlings stopped elongating within seconds regardless of nutrient status. However, seedlings cultured with higher levels of Ca2+ recovered their pre-stress rate more quickly. Correlation of enhanced recovery with enhanced root development was observed for seedlings grown in 5 mM Ca2+. Inclusion of 1.5, or 10 mM K+ in the growth matrix further reduced recovery time from ≥6 h to ≤3h. K+ effects may be due to the ability of seedlings to adjust osmotically. It previously has been shown that seedlings have significantly less K+ in their elongation zone following stress, and the osmotic potential of the expressed sap is lower than that of controls. Physiological and biophysical changes that occur in response to mechanical perturbations have potential for modification by nutritional status.This project was supported in part by NASA grant NAG10-0093.
IMMUNITY TO INFECTION BY POTATO VIRUS Y AND POTATO VIRUS X IN A Solanum phureja - S. stentomum HYBRID POTATO POPULATION
Authors: Vallejo Roger L. , Collins Wanda W. , Young J.B. , Schiavone Rocco D. 
Published: April 2024

A random sample of 6000 individuals from a recombinant Solanum phureja - S. stentomum hybrid population and 250 individuals of Solanum phureja were twice inoculated with potato virus Y (PVY) strain “o” using the air brush technique. Symptomless seedlings were field transplanted for further evaluation and 1508 seedlings were judged to be resistant to PVY (33%). At harvest, a mild selection pressure for tuber appearance was applied and 602 clones were selected.Selected clones were re-evaluated for PVY resistance in the greenhouse: twice inoculated with PVY, tested by ELISA (Enzyme-linked immunosorbent assay), graft-inoculated with tobacco PVY infected scions, and subjected to a second ELISA test. To assess immunity to infection by PVY, the ELISA negative clones were bioassayed using tobacco cv. “Burley 21” as a plant indicator. We identified 224 PVY immune clones (4.8%).Simultaneously, the first year PVY selected clones were twice inoculated with U.S. common strain of potato virus X (PVX). Clones free of PVX symptoms were tested by ELISA. Negative clones were re-inoculated with PVX and symptomless clones were re-tested by ELISA. To assess immunity to infection by PVX, negative clones were bioassayed using Gomphrena globosa as a plant indicator. We identified 7 immune (1.3%); 4 highly resistant and 4 resistant clones. Eight clones showed high levels of resistance to both PVY and PVX (high resistance to immunity).
CALIFORNIA CITRUS ROOTSTOCKS
Authors: Ferguson Louise 
Published: April 2024

Though California is currently not facing the intense disease pressure that led to the development of the Troyer and Carrizo citrange rootstocks (sweet orange × trifoliate orange hybrids) that replaced the sweet orange (Citrus sinensis [L.] Osbeck) rootstocks, the California industry is still actively involved in breeding and evaluating rootstocks.All potential rootstocks are still screened for the common fungal and viral diseases of citrus as well as for nematode susceptibility and nursery characteristics. The candidates that prove resistant are then evaluated as budded trees for scion compatibility, climatic and edaphic adaptability, and horticultural traits. Currently, rootstocks are not evaluated for salinity.Currently C-32 and C-35, citrange hybrids of `Ruby' orange and trifoliate orange (Citrus sinensis [L.] Osbeck and Poncirus trifoliata [L.] Raf.) are being evaluated with a number of navel and Valencia scions. There is also increasing interest in finding compatible productive rootstocks for new mandarin and grapefruit introductions.
FRUIT ROT CONTROL WITH BIOLOGICALLY DERIVED SUBSTANCES
Authors: Takeda Fumiomi , Janisiewicz Wojciech 
Published: April 2024

Postharvest decay represents major losses in the horticultural industry. Synthetic fungicides are an effective tool in controlling postharvest diseases. However, the need for new methods to control postharvest diseases is emphasized because of resistance to certain fungicides, increased incidence of iatrogenic diseases, health risk concerns, and regulatory constraints on pesticide usage.The use of biologically derived bioactive substances is an alternative approach for the control of postharvest diseases. For example, pyrrolnitrin, a compound produced by Pseudomonas cepacia, has high antifungal activity toward several pathogenic fungi. Chitosan, obtained from deacetylation of chitin, activates the natural defense mechanism in the host tissue. Some volatile compounds from plants and fruits have fungitoxic properties. Hinokitiol, an extract from Japanese cypress trees, is highly effective in extending the shelflife of peaches. The usefulness and limitations of a number of natural bioactive substances will be discussed.