bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nfatc2 is a primary response gene of nell-1 regulating chondrogenesis in ATDC5 cells
Authors: Chen Weiwei , Zhang Xinli , Siu Ronald K , Chen Feng , Shen Jia , Zara Janette N , Culiat Cymbeline T , Tetradis Sotirios , Ting Kang , Soo Chia 
Published: December 2010

Abstract
Nell-1 is a growth factor required for normal skeletal development and expression of extracellular matrix proteins required for bone and cartilage cell differentiation. We identified the transcription factor nuclear factor of activated T cells (Nfatc2) as a primary response gene of Nell-1 through a microarray screen, with validation using real-time polymerase chain reaction (PCR). We investigated the effects of recombinant Nell-1 protein on the chondrogenic cell line ATDC5 and primary mouse chondrocytes. The osteochondral transcription factor Runx2 was investigated as a possible intermediary between Nell-1 and Nfatc2 using adenoviral overexpression of wild-type and dominant-negative Runx2. Nell-1 transiently induced both transcription and translation of Nfatc2, an effect inhibited by transduction of dominant-negative Runx2, suggesting that Runx2 was necessary for Nfatc2 induction. Differentiation assays revealed inhibitory effects of Nell-1 on ATDC5 cells. Although proliferation was unaffected, expression of chondrocyte-specific genes was decreased, and cartilage nodule formation and proteoglycan accumulation were suppressed. siRNA knockdown of Nfatc2 significantly reversed these inhibitory effects. To elucidate the relationship between Nell-1, Runx2, and Nfatc2 in vivo, their presence and distribution were visualized in femurs of wild-type and Nell1-deficient mice at both neonatal and various developmental stages using immunohistochemistry. All three proteins colocalized in the perichondrium of wild-type femurs but stained weakly or were completely absent in Nell1-deficient femurs at neonatal stages. Thus Nfatc2 likely plays an important role in Nell-1-mediated osteochondral differentiation in vitro and in vivo. To our knowledge, this is the first demonstration that Nfatc2 is a primary response gene of Nell-1. © 2011 American Society for Bone and Mineral Research.
Selective venous catheterization for the localization of phosphaturic mesenchymal tumors
Authors: Andreopoulou Panagiota , Dumitrescu Claudia E , Kelly Marilyn H , Brillante Beth A , Cutler Peck Carolee M , Wodajo Felasfa M , Chang Richard , Collins Michael T 
Published: December 2010

Abstract
Tumor-induced osteomalacia (TIO) is characterized by renal phosphate wasting, hypophosphatemia, and aberrant vitamin D3 metabolism and is caused by fibroblast growth factor 23 (FGF-23)–producing mesenchymal tumors, which are often difficult to locate. We investigated the utility of selective venous sampling in tumor localization. The primary endpoint was identification of the FGF-23 concentration ratio between the venous drainage of the tumor bed and the general circulation that was diagnostic of the location of an FGF-23-secreting tumor. Fourteen subjects underwent 15 sampling procedures after functional and anatomic imaging studies. Subjects fit into three imaging categories: no suspicious site, multiple sites, and single site (positive controls). FGF-23 levels were measured by ELISA. Suspicious tumors were resected for diagnosis, confirmation, and cure. In subjects with a positive venous sampling study and subsequent cure, a minimum ratio of 1.6 was diagnostic. In 7 of 14 subjects there was suggestive imaging, a diagnostic ratio, and an associated TIO tumor (true positive). Four of these required complicated resection procedures. In 4 of 14 subjects with no suspicious site on imaging studies, an FGF-23 diagnostic ratio was not detected (true negative). Biopsy or resection of a single lesion in 2 of 14 subjects with a diagnostic ratio failed to identify a TIO tumor (false positive). A diagnostic FGF-23 ratio was absent in 1 of 14 subjects whose tumor was a single highly suspicious lesion on imaging studies (false negative). These data yield a sensitivity of 0.87 [95% confidence interval (CI) 0.47–0.99] and a specificity of 0.71 (95% CI 0.29–0.96). Selective venous sampling for FGF-23 was particularly useful in subjects with multiple suspicious sites or an anatomically challenging planned resection but not in the absence of a suspicious lesion on imaging studies. © 2011 American Society for Bone and Mineral Research.
Genome-scale techniques highlight the epigenome and redefine fundamental principles of gene regulation
Authors: Pike J Wesley 
Published: December 2010

Abstract
The regulation of gene expression represents one of the most fundamental of biologic processes that controls cellular proliferation, differentiation, and function. Recent technological advances in genome-wide annotation together with bioinformatic/computational analyses have contributed significantly to our understanding of transcriptional regulation at the epigenomic and regulomic levels. This perspective outlines the techniques that are being utilized and summarizes a few of the outcomes. © 2011 American Society for Bone and Mineral Research.
Cross-sectional analysis of the association between fragility fractures and bone microarchitecture in older men: The STRAMBO study
Authors: Szulc Pawel , Boutroy Stéphanie , Vilayphiou Nicolas , Chaitou Ali , Delmas Pierre D , Chapurlat Roland 
Published: December 2010

Abstract
Areal bone mineral density (aBMD) measured by dual-energy X-ray absorptiometry (DXA) identifies 20% of men who will sustain fragility fractures. Thus we need better fracture predictors in men. We assessed the association between the low-trauma prevalent fractures and bone microarchitecture assessed at the distal radius and tibia by high-resolution peripheral quantitative computed tomography (HR-pQCT) in 920 men aged 50 years of older. Ninety-eight men had vertebral fractures identified on the vertebral fracture assessment software of the Hologic Discovery A device using the semiquantitative criteria, whereas 100 men reported low-trauma peripheral fractures. Men with vertebral fractures had poor bone microarchitecture. However, in the men with vertebral fractures, only cortical volumetric density (D.cort) and cortical thickness (C.Th) remained significantly lower at both the radius and tibia after adjustment for aBMD of ultradistal radius and hip, respectively. Low D.cort and C.Th were associated with higher prevalence of vertebral fractures regardless of aBMD. Severe vertebral fractures also were associated with poor trabecular microarchitecture regardless of aBMD. Men with peripheral fractures had poor bone microarchitecture. However, after adjustment for aBMD, all microarchitectural parameters became nonsignificant. In 15 men with multiple peripheral fractures, trabecular spacing and distribution remained increased after adjustment for aBMD. Thus, in men, vertebral fractures and their severity are associated with impaired cortical bone, even after adjustment for aBMD. The association between peripheral fractures and bone microarchitecture was weaker and nonsignificant after adjustment for aBMD. Thus bone microarchitecture may be a determinant of bone fragility in men, which should be investigated in prospective studies. © 2011 American Society for Bone and Mineral Research.
Electronic ground states and vibrational frequency shifts of diatomic ligands in heme adducts
Authors: Liu Yang , Sun Huai 
Published: December 2010

AbstractDFT calculations were carried out to study heme complexes with diatomic ligand (CO, NO, or O2) and trans‐imidazole ligand. The optimized electronic ground states of CO, NO, and O2 adducts are singlet, doublet, and open‐shell singlet, respectively. For O2 adduct, the open‐shell singlet is slightly lower in energy than the close‐shell singlet. However, important differences are found in optimized structures and vibrational frequencies. Particularly, the trans‐imidazole‐induced frequency up‐shift of the FeO(O) stretching mode can be predicted only with the open‐shell singlet as ground state. An analysis of normal modes confirms that the up‐shifts in the bent (NO and O2) adducts are mainly due to mixing of FeX(O) stretching mode with FeXO bending coordinate. Our study of binding mechanism indicates that a secondary source of the upshifts is the diminished weakening of the FeX(O) bonds. The FeX(O) bond strengths are modulated by σ competition mechanism, which weakens the FeX(O) bond and σ–π cooperation mechanism, which only exists in the bent adducts and enforce the FeX(O) bond. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2011
Theory for trivial trajectory parallelization of multicanonical molecular dynamics and application to a polypeptide in water
Authors: Ikebe Jinzen , Umezawa Koji , Kamiya Narutoshi , Sugihara Takanori , Yonezawa Yasushige , Takano Yu , Nakamura Haruki , Higo Junichi 
Published: December 2010

AbstractTrivial trajectory parallelization of multicanonical molecular dynamics (TTP‐McMD) explores the conformational space of a biological system with multiple short runs of McMD starting from various initial structures. This method simply connects (i.e., trivially parallelizes) the short trajectories and generates a long trajectory. First, we theoretically prove that the simple trajectory connection satisfies a detailed balance automatically. Thus, the resultant long trajectory is regarded as a single multicanonical trajectory. Second, we applied TTP‐McMD to an alanine decapeptide with an all‐atom model in explicit water to compute a free‐energy landscape. The theory imposes two requirements on the multiple trajectories. We have demonstrated that TTP‐McMD naturally satisfies the requirements. The TTP‐McMD produces the free‐energy landscape considerably faster than a single‐run McMD does. We quantitatively showed that the accuracy of the computed landscape increases with increasing the number of multiple runs. Generally, the free‐energy landscape of a large biological system is unknown a priori. The current method is suitable for conformational sampling of such a large system to reduce the waiting time to obtain a canonical ensemble statistically reliable. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2011
Growth factors controlling ovarian functions
Authors: Sirotkin Alexander V. 
Published: December 2010

AbstractThe present review demonstrates an important role of different growth factors (of insulin‐like growth factors, epidermal growth factors, vascular endothelial growth factor, thrombopoietin, erytropoietin, hepatocyte growth factor, and growth factors of Hedgehog, Wnt and Notch families) in control of different ovarian functions—ovarian cell proliferation, apoptosis, folliculogenesis, luteogenesis, oogenesis, release of hormones, response to upstream hormonal regulators, fertility and, in some cases, in development of ovarian disorders. The possibility of practical application of these growth factors for characterization, prediction, and regulation the ovarian state is demonstrated. J. Cell. Physiol. 226: 2222–2225, 2011. © 2010 Wiley‐Liss, Inc.
Knockdown of CITED2 using short‐hairpin RNA sensitizes cancer cells to cisplatin through stabilization of p53 and enhancement of p53‐dependent apoptosis
Authors: Wu Zchong‐Zcho , Sun Nian‐Kang , Chao Chuck C.‐K. 
Published: December 2010

AbstractCITED2 is a transcriptional modulator which has been implicated in human oncogenesis. In the present study, we examined whether CITED2 is also involved in the resistance of cancer cells to the chemotherapeutic drug cisplatin. We first observed that knockdown of CITED2 using short‐hairpin RNA sensitized non‐tumorigenic HEK293 cells to cisplatin. Sensitization to cisplatin following knockdown of CITED2 was also observed in cervical carcinoma HeLa cells and in cisplatin‐resistant HeLa cells, thereby showing that acquired cisplatin resistance could be reversed by CITED2 knockdown. This sensitization response was dependent on the status of p53 since efficient sensitization was observed in p53‐positive hepatocellular carcinoma (HCC) Sk‐Hep‐1 cells, whereas a negligible response was produced in the two p53‐defective cell lines HCC Mahlavu and lung cancer H1299. In contrast, overexpression of CITED2 decreased sensitivity of HEK293 cells to cisplatin, while moderate resistance was produced in HeLa cells. Overexpression of CITED2 also decreased sensitivity to cisplatin in p53‐defective H1299 cells when exogenous p53 expression was re‐introduced. We observed that knockdown of CITED2‐induced CBP/p300‐mediated p53 acetylation (Lys373) in HEK293 cells, thereby leading to a decrease of p53 ubiquitination and subsequent accumulation of the p53 protein. Notably, the effects of CITED2 knockdown on p53 accumulation and the increase of p53's target Bax were more pronounced after treatment with cisplatin. Based on these results, we propose that a combination of cisplatin and CITED2 shRNA may represent an effective treatment against p53‐sensitive cancer cells. J. Cell. Physiol. 226: 2415–2428, 2011. © 2010 Wiley‐Liss, Inc.
Utilization of laser‐assisted analytical methods for monitoring of lead and nutrition elements distribution in fresh and dried Capsicum annuum l. leaves
Authors: Galiová Michaela , Kaiser Jozef , Novotný Karel , Hartl Martin , Kizek Rene , Babula Petr 
Published: December 2010

AbstractLaser induced breakdown spectroscopy (LIBS) and laser ablation inductively coupled plasma mass spectrometry (LA‐ICP‐MS) have been applied for high‐resolution mapping of accumulation and distribution of heavy metal (lead) and nutrition elements (potassium, manganese) in leaves of Capsicum annuum L. samples. Lead was added in a form of Pb(NO3)2 at concentration up to 10 mmol L−1 into the vessels that contained tap water and where the 2‐months old Capsicum annuum L. plants were grown another seven days. Two dimensional maps of the elements are presented for both laser‐assisted analytical methods. Elemental mapping performed on fresh (frozen) and dried Capsicum annuum L. leaves are compared. Microsc. Res. Tech., 2011. © 2010 Wiley‐Liss, Inc.
An efficient and clean Michael addition of indoles to electron‐deficient olefins under solvent‐ and catalyst‐free condition
Authors: Liu Xiong‐Li , Xue Dong , Zhang Zun‐Ting 
Published: December 2010

AbstractAn efficient Michael addition of indoles to electron‐deficient olefins under solvent‐ and catalyst‐free condition afforded biologically important 3‐substituted indole derivatives in good to excellent yields was reported. The acidic NH proton of indole plays a key role in Michael addition of indoles to electron‐deficient olefins. This very simple procedure provides an efficient and clean process for the synthesis of indole derivatives. J. Heterocyclic Chem., (2011).
Comparative internal structure of dorsal lips and radiolar appendages in sabellidae (Polychaeta) and phylogenetic implications
Authors: Capa María , Nogueira João Miguel de Matos , Rossi Maíra Cappellani Silva 
Published: December 2010

AbstractFan worms (Sabellidae) possess paired modified prostomial structures at the base of the radiolar crown, dorso‐lateral to the mouth, called dorsal lips. The dorsal lips are involved in the sorting of particles collected by the radiolar crown. The range of variation in the morphology of dorsal lips is extensive, and probably this is not only due to adaptations to different environments and feeding preferences but also due to phylogenetic constraints. In this study, we describe and compare the morphology of dorsal lips in a range of sabellid taxa based on histological cross‐sections of these structures, and compare our data and terminology with those of previous studies. Dorsal lips are maintained erect in most taxa by a modified radiole fused to them known as dorsal radiolar appendage. We suggest that dorsal radiolar appendages with an internal supporting axis (cellular or acellular) and probably also the ventral lips are synapomorphies of the family. J. Morphol., 2011. © 2010 Wiley‐Liss, Inc.
Portal vein embolization (prior to major hepatectomy) effects on regeneration, resectability, and outcome
Authors: Abdalla Eddie K. 
Published: December 2010

AbstractPortal vein embolization (PVE) is used to increase the volume and function of the liver that will remain after extensive liver resection. Operative outcomes are improved in properly selected patients who undergo PVE and experience adequate future liver remnant (FLR) hypertrophy. Absolute volume and volume change of the FLR after PVE (interpreted in context of liver disease) predict adequate liver function postresection. Oncologic outcomes following resection in patients with appropriately applied PVE are excellent. J. Surg. Oncol. 2010;102:960–967. © 2010 Wiley‐Liss, Inc.
Outcomes of laparoscopic hepatic resection for colorectal cancer metastases
Authors: Nguyen Kevin Tri , Geller David A. 
Published: December 2010

AbstractThe role of laparoscopic liver resection for cancer remains controversial. This review summarizes the expanding literature on outcomes of minimally invasive hepatic resection for colorectal cancer liver metastases. Four recent studies (in more than 300 patients) show 5‐year overall‐survival rates of 46–64%, which are comparable to results in modern open hepatic resection series. The advantages of laparoscopic liver resection include smaller incisions, less pain, less narcotic requirements, and shorter length of stay. J. Surg. Oncol. 2010;102:975–977. © 2010 Wiley‐Liss, Inc.
Optimum imaging of colorectal metastases
Authors: Grand David J. , Beland Michael , Noto Richard B. , Mayo‐Smith William 
Published: December 2010

AbstractDramatic improvements in diagnostic imaging have developed with and enabled increasingly sophisticated treatments for metastatic colorectal cancer. Advances in therapeutic techniques, such as surgical resection and percutaneous therapies, demand that diagnostic imaging provide an accurate assessment of disease burden as well as precise localization. In this article, we present the current state‐of‐the‐art of diagnostic imaging for evaluation of metastatic colorectal cancer. J. Surg. Oncol. 2010;102:909–913. © 2010 Wiley‐Liss, Inc.
Radiofrequency ablation of colorectal hepatic metastases
Authors: Guenette Jeffrey P. , Dupuy Damian E. 
Published: December 2010

AbstractRadiofrequency ablation (RFA) may be performed intraoperatively, laparoscopically, or percutaneously. The percutaneous approach is associated with the least procedural risk and may be performed under local anesthesia. Percutaneous RFA should be considered a primary treatment option for patients with unresectable hepatic tumors or conditions that prohibit general anesthesia or abdominal surgery. Continually improving thermal ablation and imaging technologies are likely to further increase tumor ablation efficacy and expand its role in treatment of hepatic metastases. J. Surg. Oncol. 2010;102:978–987. © 2010 Wiley‐Liss, Inc.
The neoadjuvant therapy of colorectal hepatic metastases and the role of biologic sensitizing and resistance factors
Authors: Wanebo Harold J. , Berz David 
Published: December 2010

AbstractLiver metastasis represents a common systemic complication of colorectal cancers (CRCs). Partial liver resection has been demonstrated to result in long‐term survival in certain well‐selected patients with otherwise well‐controlled systemic disease. Neoadjuvant therapy has been demonstrated to result in improved resectability and potentially longer survival in patients with liver metastases from CRC. The addition of biologic agents to chemotherapy has been shown to improve response rates and overall survival in patients with metastatic CRC. Here, we are discussing the role of biologic agents in the treatment of patients with liver metastases from CRC. We also discuss the role of biomarkers for response and resistance to such novel therapies. J. Surg. Oncol. 2010;102:891–897. © 2010 Wiley‐Liss, Inc.
Treatment of liver colorectal metastases: Role of laparoscopy, radiofrequency ablation, and microwave coagulation
Authors: Rocha Flavio G. , D'Angelica Michael 
Published: December 2010

AbstractUp to 50% of patients with colorectal cancer will develop metastatic disease in the liver. While surgical extirpation remains the best option for long‐term survival, several complementary modalities such as laparoscopy, radiofrequency ablation, and microwave coagulation have gained wide acceptance as primary and adjunct therapies for both resectable and unresectable disease. This review will focus on the application and outcome of these techniques in patients with colorectal liver metastases. J. Surg. Oncol. 2010;102:968–974. © 2010 Wiley‐Liss, Inc.
Evolution of neoadjuvant therapy for extended hepatic metastases—have we reached our (non‐resectable) limit?
Authors: Adam René , Hoti Emir , Bredt Luis César 
Published: December 2010

AbstractSurgical resection offers the best chance for cure in patients with colorectal liver metastases; however, only 15–25% of them can benefit from surgery. To increase resectability a number of strategies have been developed in different fields including chemotherapy, surgery, and radiology. Bringing them together into an integrated framework has expanded the number of patients that can be treated with curative intent. This review focuses on recent oncosurgical changes, their impact, and future directions. J. Surg. Oncol. 2010;102:922–931. © 2010 Wiley‐Liss, Inc.
Prognosticators in thigh soft tissue sarcomas
Authors: Newcomer Anne E. , Dylinski Diane , Rubin Brian P. , Joyce Michael J. , Hoeltge Gerald , Bershadsky Boris , Lietman Steven A. 
Published: December 2010

AbstractBackgroundIn sarcoma patients the roles of smoking history, family cancer history, and leukoreduced blood transfusions have not been studied and the effect of preoperative radiation on blood loss has not been examined.MethodsSeventy‐seven patients with non‐metastatic and non‐recurrent thigh sarcomas surgically treated at the Cleveland Clinic were identified. Among patient variables studied were: close family history of cancer, perioperative transfusion history, smoking history, and radiation history. Median follow‐up for the survivors was 3.2 years.ResultsWe found that tumor grade, transfusion >3 U (P = 0.022), and pre‐ or post‐operative radiation therapy (P = 0.041) were risk factors for distant metastasis. Tumor grade (P = 0.008), positive smoking history (P = 0.039), and >3 U of non‐leukoreduced blood transfused (P = 0.037) were risk factors for death of any‐cause. Close family history of cancer correlated with having a grade 3 sarcoma (P = 0.044). Neoadjuvant radiotherapy correlated with >3 U of blood transfused (P = 0.001) and biopsy performed at the treating institution led to a significant decrease in rate of recurrence (P = 0.016).ConclusionsWe present novel findings in terms of transfusions, family history of cancer and site of initial biopsy in sarcoma patients. J. Surg. Oncol. 2011;103:85–91. © 2010 Wiley‐Liss, Inc.
Pathobiology of colorectal cancer hepatic metastases with an emphasis on prognostic factors
Authors: LeGolvan Mark P. , Resnick Murray 
Published: December 2010

AbstractColorectal cancer is the second leading cause of cancer related death in the United States. The majority of these deaths are due to metastasis, with the liver easily accounting as the most common site of deposit. While there are multiple steps in the CRC hepatic metastatic cascade, this review attempts to summarize the different processes involved, focusing on the most recent discoveries, as well as the associated effects in relation to prognosis. J. Surg. Oncol. 2010;102:898–908. © 2010 Wiley‐Liss, Inc.