bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Yeast Ubr1 Ubiquitin Ligase Participates in a Prominent Pathway That Targets Cytosolic Thermosensitive Mutants for Degradation
Authors: Khosrow-Khavar Farzin , Fang Nancy N , Ng Alex H M , Winget Jason M , Comyn Sophie A , Mayor Thibault 
Published: May 2012

Abstract
Mutations causing protein misfolding and proteolysis are associated with many genetic diseases. The degradation of these aberrant proteins typically is mediated by protein-quality control pathways that recognize misfolded domains. Several E3 ubiquitin ligases have been shown to target cytosolic misfolded proteins to the proteasome. In this study, we characterized a panel of more than 20 cytosolic thermosensitive mutants from six essential genes in Saccharomyces cerevisiae. These wild-type proteins are stable at restrictive temperature. In contrast, we found that a large portion of the mutants is degraded at nonpermissive temperature in a proteasome-dependent manner. Approximately one-third of the assessed unstable mutants are targeted by the Ubr1 ubiquitin ligase. In two cases, efficient degradation of the thermosensitive mutants is abrogated in the absence of Ubr1 alone, whereas in a third case it is reliant on the dual deletion of Ubr1 and the nuclear E3 ligase San1. We found that the impairment of the degradation of these quality control substrates at the restrictive temperature is associated with the suppression of thermosensitive phenotype. This study confirms that Ubr1 plays an important role in the degradation of cytosolic misfolded proteins and indicates that degradation mediated by protein quality control is a major cause for the conditional lethality of mutated essential genes.
Regulated Expression of Chromobox Homolog 5 Revealed in Tumors of ApcMin/+ ROSA11 Gene Trap Mice
Authors: Thliveris Andrew T , Clipson Linda , Sommer Lacy L , Schoenike Barry A , Hasenstein Jason R , Schlamp Cassandra L , Alexander Caroline M , Newton Michael A , Dove William F , Amos-Landgraf James M 
Published: May 2012

Abstract
The gene-trap lacZ reporter insertion, ROSA11, in the Cbx5 mouse gene illuminates the regulatory complexity of this locus in ApcMin/+ mice. The insertion site of the β-Geo gene-trap element lies in the 24-kb intron proximal to the coding region of Cbx5. Transcript analysis indicates that two promoters for Cbx5 flank this insertion site. Heterozygotes for the insertion express lacZ widely in fetal tissues but show limited expression in adult tissues. In the intestine, strong expression is limited to proliferative zones of crypts and tumors. Homozygotes for ROSA11, found at a lower than Mendelian frequency, express reduced levels of the coding region transcript in normal tissues, using a downstream promoter. Analysis via real-time polymerase chain reaction indicates that the upstream promoter is the dominant promoter in normal epithelium and tumors. Bioinformatic analysis of the Cbx5 locus indicates that WNT and its target transcription factor MYC can establish a feedback loop that may play a role in regulating the self-renewal of the normal intestinal epithelium and its tumors.
Mating-Type Genes andMATSwitching inSaccharomyces cerevisiae
Authors: Haber James E 
Published: May 2012

AbstractMating type in Saccharomyces cerevisiae is determined by two nonhomologous alleles, MATa and MATα. These sequences encode regulators of the two different haploid mating types and of the diploids formed by their conjugation. Analysis of the MATa1, MATα1, and MATα2 alleles provided one of the earliest models of cell-type specification by transcriptional activators and repressors. Remarkably, homothallic yeast cells can switch their mating type as often as every generation by a highly choreographed, site-specific homologous recombination event that replaces one MAT allele with different DNA sequences encoding the opposite MAT allele. This replacement process involves the participation of two intact but unexpressed copies of mating-type information at the heterochromatic loci, HMLα and HMRa, which are located at opposite ends of the same chromosome-encoding MAT. The study of MAT switching has yielded important insights into the control of cell lineage, the silencing of gene expression, the formation of heterochromatin, and the regulation of accessibility of the donor sequences. Real-time analysis of MAT switching has provided the most detailed description of the molecular events that occur during the homologous recombinational repair of a programmed double-strand chromosome break.
1% Alendronate Gel as Local Drug Delivery in the Treatment of Class II Furcation Defects: A Randomized Controlled Clinical Trial
Authors: Pradeep A.R. , Kumari Minal , Rao Nishanth S. , Naik Savitha B. 
Published: May 2012

Background: Alendronate (ALN), an aminobisphosphonate, is known to stimulate the formation of osteoblast precursors to promote osteoblastogenesis. The present study aims to explore the efficacy of 1% ALN gel as a local drug delivery system in adjunct to scaling and root planing (SRP) for the treatment of Class II furcation defects in comparison with placebo gel.Methods: A total of 69 mandibular Class II furcation defects were randomized and treated with either 1% ALN gel or placebo gel. Clinical parameters were recorded at baseline, 3 months, 6 months, and 12 months, and radiographic parameters were recorded at baseline, 6 months, and 12 months. Defect fill at baseline, 6 months, and 12 months was calculated on standardized radiographs using image analysis software.Results: Mean probing depth (PD) reduction and mean relative vertical (RVCAL) and horizontal (RHCAL) clinical attachment level gain were shown to be greater in the ALN group than the placebo group at 3, 6, and 12 months. Furthermore, a significantly greater mean percentage of bone fill was found in the ALN group (32.11% ± 6.18%, 32.66% ± 5.86%), compared with the placebo group (2.71% ± 0.61%, 1.83% ± 1.51%), at 6 and 12 months, respectively.Conclusions: The results of the present study show that local delivery of 1% ALN into a Class II furcation defect stimulates a significant PD reduction, RVCAL and RHCAL gains, and improved bone fill compared with placebo gel as an adjunct to SRP. ALN can provide a new direction in management of furcation defects.
Application of Multivariate Methods to Optimize Development of Thin Oil Zones in a Mature Carbonate Reservoir
Authors: Al-Otaibi Majid H. , Fischbuch D. Brett , Taibah Obai , Al-Julaih Ali H. 
Published: May 2012

Abstract
Exploitation of thin oil zones in a mature field with complex carbonate geology under strong water drive offers many challenges. The primary objective is effective oil recovery from the thin oil zones without excessive water production. The initial development phase targeting thin remaining oil zones in a giant, mature carbonate field in Saudi Arabia has been guided by reservoir simulation results, with performance generally exceeding expectations. However, performance of individual horizontal wells has varied greatly. Multivariate statistical methods have been applied across the gamut of reservoir parameters for these wells to gain further insights into critical success factors and mechanisms. Response variables were established (producing time to reach various watercut thresholds) to gauge well performance. Principal component, factor, and multiple regression analyses were applied to independent reservoir parameters for a population of 20 horizontal wells placed in the target zone. These parameters included zone thickness, standoff from fluid contacts, vertical permeability contrast, thickness of low-permeability interval, reservoir contact, net/gross ratio, completion design, extent of fracturing, zone porosity, proximity to injectors, and trajectory orientation. Multivariate analysis conclusively demonstrated that the principal factor governing well performance in the early period (up to three years) was the vertical permeability contrast or in other words, the extent to which a permeability baffle exists between the thin low-permeability zone and the underlying thick high-permeability zone. Other parameters may contribute to well performance beyond the 30% watercut threshold and will be addressed in a future paper. The findings from this study have been translated into Best Practices for exploiting thin oil zones and have been applied in further developing the thin oil zone in the subject field.
A Finite Element Method for a Noncoercive Elliptic Problem with Neumann Boundary Conditions
Authors: Kavaliou Klim , Tobiska Lutz 
Published: May 2012

Abstract We apply a combined finite-element finite-volume method on a noncoercive
elliptic boundary value problem. The proposed method is based on
triangulations of weakly acute type and a secondary
circumcentric subdivision. The properties of the continuous problem,
that the kernel is one-dimensional and spanned by a positive function,
are preserved in the discrete case. A priori error estimates of first
order in the H¹-norm are shown for sufficiently small mesh sizes.
Numerical test examples confirm the theoretical predictions.
Numerical Experiments for a Singularly Perturbed Parabolic Problem with Degenerating Convective Term and Discontinuous Source
Authors: Clavero Carmelo , Gracia Jose L. , Shishkin Grigorii , Shishkina Lidia 
Published: May 2012

Abstract A finite difference scheme on special piecewise-uniform grids
condensing in the interior layer is constructed for a singularly
perturbed parabolic convection-diffusion equation with a
discontinuous right-hand side and a multiple degenerating convective
term (the convective flux is directed into the domain).
When constructing the scheme, monotone grid approximations, similar
to those developed and justified earlier by authors for a problem
with a simple degenerating convective term, are used. Using the
known technique of numerical experiments on embedded meshes, it is
numerically verified that the constructed scheme converges
ε-uniformly in the maximum norm at the convergence rate
close to one.
An Attendance Behavior Model At Sports Events: Comparison and Constrast of Two Models
Authors: Santos Manuel 
Published: May 2012

An Attendance Behavior Model At Sports Events: Comparison and Constrast of Two Models
The purpose of this research is to improve our understanding of consumer behavior in the context of sporting events by means of the use of two models which are widely used in the marketing literature: one of them is based on the planned behavior theory, the other one is based on the expectation disconfirmation theory. Both models contribute to the research on satisfaction from a cognitive-affective point of view, integrating the study of emotions, motivations and consumer satisfaction. For the study a survey was undertaken at a stadium of a professional sporting entity. The contrast between the proposed hypotheses (n=205) was carried out using factor analysis (FA) and structural equation systems (SEM). The obtained results allow us both to recognize the discriminating and converging validity of the studied dimensions and to learn the important differences in the influence that each of them has on the intentions of future behavior.
Social Aspects of the Athletes and Their Behavior in Society
Authors: Kosík Miloš 
Published: May 2012

Social Aspects of the Athletes and Their Behavior in Society
This article shows documents from the Czech Republic as well as from other countries dealing with sports codex. Sportsmen, sports clubs, managers and many other people who work in sport they all are concerned about Ethic Codex. For the articles I used quantitative methods, data collection methods and the Ethnographic Method.
Success rate of repeated fine needle aspiration biopsy of clinically suspicious thyroid nodules
Authors: Farahati J. , Görges R. , Grabellus F. , Bockisch A. , Sheu-Grabellus S.-Y. , Nagarajah J. 
Published: May 2012

SummaryIn this study we evaluated the success rate of double fine needle aspiration biopsy (FNAB) of clinically suspicious thyroid nodules in one session. Aim: The success rate of FNAB in clinical setting is quite low. There were several attempts made to improve the success rate of this method. It is anticipated that a double FNAB in one session would increase the success rate of FNAB. Patients, methods: 176 consecutive patients (130 women, 46 men; mean age 56 years ± 11) with at least one clinically suspicious nodule were included in this study. Each individual nodule was biopsied twice (20G- and 21G-needle). In 33 patients, two suspicious nodules were biopsied, accounting for a total of 209 biopsied thyroid nodules. To evaluate the success rate the number of cell formations and the total number of cells in each cell formation were counted. Results: The biopsy with the 20G needle provided in mean 40 cell cluster with a mean of 830 cells niedwhereas the 21G needle provided in mean 41 cell cluster with a mean of 1010 cells. With the 20G needle the success rate was 73%, with the 21G needle 78% and the combination of the both biopsies provided a success rate of 87% (p = 0.01). Based on the number of cell formations and the total number of cells, the difference between the two needle sizes was not significant (p = 0.5 for cell formations and p = 0.9 for the total number of cells, respectively). Conclusion: A double FNAB of suspicious thyroid nodules in one session provides a higher success rate, and a 21G needle is sufficient enough.
Soluble Trem-like Transcript-1 Regulates Leukocyte Activation and Controls Microbial Sepsis
Authors: Derive Marc , Bouazza Youcef , Sennoun Nacira , Marchionni Sandra , Quigley Laura , Washington Valance , Massin Frédéric , Max Jean-Pierre , Ford Jill , Alauzet Corentine , Levy Bruno , McVicar Daniel W. , Gibot Sébastien 
Published: May 2012

Abstract
The triggering receptor expressed on myeloid cells (TREM)-1 plays a crucial role during the onset of sepsis by amplifying the host immune response. The TREM-like transcript-1 (TLT-1) belongs to the TREM family, is selectively expressed on activated platelets, and is known to facilitate platelet aggregation through binding to fibrinogen. In this study, we show that a soluble form of TLT-1 is implicated in the regulation of inflammation during sepsis by dampening leukocyte activation and modulating platelet-neutrophil crosstalk. A 17-aa sequence of the TLT-1 extracellular domain (LR17) is responsible for this activity through competition with the TREM-1 ligand. Whereas early or late LR17 treatment of septic mice improves survival, treml-1−/− animals are highly susceptible to polymicrobial infection. The present findings identify platelet-derived soluble TLT-1 as a potent endogenous regulator of sepsis-associated inflammation and open new therapeutic perspectives. We anticipate soluble TLT-1 to be important in regulating leukocyte activation during other noninfectious inflammatory disorders.
Runx1 Deficiency in CD4+ T Cells Causes Fatal Autoimmune Inflammatory Lung Disease Due to Spontaneous Hyperactivation of Cells
Authors: Wong Won Fen , Kohu Kazuyoshi , Nakamura Akira , Ebina Masahito , Kikuchi Toshiaki , Tazawa Ryushi , Tanaka Keisuke , Kon Shunsuke , Funaki Tomo , Sugahara-Tobinai Akiko , Looi Chung Yeng , Endo Shota , Funayama Ryo , Kurokawa Mineo , Habu Sonoko , Ishii Naoto , Fukumoto Manabu , Nakata Koh , Takai Toshiyuki , Satake Masanobu 
Published: May 2012

Abstract
The Runx1 transcription factor is abundantly expressed in naive T cells but rapidly downregulated in activated T cells, suggesting that it plays an important role in a naive stage. In the current study, Runx1−/−Bcl2tg mice harboring Runx1-deleted CD4+ T cells developed a fatal autoimmune lung disease. CD4+ T cells from these mice were spontaneously activated, preferentially homed to the lung, and expressed various cytokines, including IL-17 and IL-21. Among these, the deregulation of IL-21 transcription was likely to be associated with Runx binding sites located in an IL-21 intron. IL-17 produced in Runx1-deleted cells mobilized innate immune responses, such as those promoted by neutrophils and monocytes, whereas IL-21 triggered humoral responses, such as plasma cells. Thus, at an initial stage, peribronchovascular regions in the lung were infiltrated by CD4+ lymphocytes, whereas at a terminal stage, interstitial regions were massively occupied by immune cells, and alveolar spaces were filled with granular exudates that resembled pulmonary alveolar proteinosis in humans. Mice suffered from respiratory failure, as well as systemic inflammatory responses. Our data indicate that Runx1 plays an essential role in repressing the transcription of cytokine genes in naive CD4+ T cells and, thereby, maintains cell quiescence.
Influence of the Grander Technology in the Physical Properties of the Self-Etch Adhesive System
Authors: Barcellos Daphne Câmara , Torres Carlos Rocha Gomes , de Paiva Gonçalves Sergio Eduardo , Balducci Ivan , da Silva Tânia Mara , Matias Diogo Toledo , Lima Huhtala Maria Filomena Rocha 
Published: May 2012

ABSTRACT

Aim
The objective of this study was to evaluate the influence of the Grander technology in reducing the surface tension and contact angle of self-etch adhesive system.

Materials and methods
Distilled water and Xeno III self-etch adhesive system (Dentsply) were modified by physical contact with the flexible unit Grander system to revitalize water, for 48 hours, resulting in four groups: Group CW-distilled water under normal conditions; Group CA-Xeno III adhesive system under normal conditions; Group GW-distilled water system modified by the Grander system; Group GA-Xeno III adhesive system modified by the Grander system. Surface tension and contact angle of the adhesive system and water in normal and Grandermodified conditions was measured with a goniometer. ANOVA and Tukey test were used to analyze the results (5%).

Results and conclusion
For surface tension, ANOVA showed p < 0.05, what indicated significant differences between the groups. The mean values (D/cm) for the groups were: CW-72.4 a; GW-69.45 b; GA: 31.17 c; CA-29.98 c. The results showed a significant reduction of surface tension for distilled water modified by Grander system. Grander technology did not interfere in the surface tension and contact angle physical properties of the Xeno III self-etch adhesive system.

Clinical significance
Grander technology is a method that revitalizes water by physical spatial restructuring of its molecules. The modify of an adhesive system, also a liquid with an aqueous solvent, can provide a reduction in surface tension and contact angle, increasing its wetness capacity and therefore ensuring a greater diffusibility.

How to cite this article
de Paiva Gonçalves SE, Matias DT, Barcellos DC, Lima Huhtala MFR, da Silva TM, Balducci I, Torres CRG. Influence of the Grander Technology in the Physical Properties of the Self-Etch Adhesive System. World J Dent 2012;3(1):1-5.
Patient Attendance for Emergency Care in a Brazilian Dental School
Authors: Torres Carlos Rocha Gomes , Gomes Ana Paula Martins , Balducci Ivan , Tiradentes Naiara , dos Santos Ilana Souza Serafim , de Vasconcelos Rafaela Andrade , Kubo Claudio Hideki , Almeida Janete Dias 
Published: May 2012

ABSTRACTObjectiveThe objective of this study was to analyze the profile of patients attending the Emergency Dental Clinic at São José dos Campos Dental School, UNESP-São Paulo State University, between 2006 and 2010, evaluating the gender, ethnicity, age, diagnosis and treatment performed for resolution of the cases.Materials and methodsA total of 600 dental records were analyzed by descriptive statistics, Fisher and Chi-square tests at a significance level of 5%. The dental records included information on the patients’ name, gender, age, ethnicity, phone number, date of attendance, diagnosis and treatment performed. The case history addressed the chief complaint, medical and dental history of the patient. The clinical evaluation comprised tests, such as visual examination of the intraoral hard and soft tissues, tooth percussion, palpation, probing, pulp sensitivity test and radiographic examination. The diagnosis and treatment for each case were established based on the case history and clinical tests.ResultsThere was predominance of female gender (59.17%) and patients aged 20 to 39 years (49.67%). Most procedures were performed to solve endodontic emergencies (37.5%), followed by placement of dressings (10.6%), tooth extraction (8.9%), periodontal problems (4.6%) and cementation of provisional crowns or definitive dentures (4.5%).ConclusionDental caries and its consequences were the main factors involved in the emergency attendances. There is a constant need of health promotion, considering that the patients searched for restorative treatments without the necessary emphasis on prevention. Additional studies are needed to determine strategies for decreasing the use of emergency services for nonemergency dental problems.Clinical relevanceThe diagnosis and treatment of dental emergencies are challenging. If inadequately performed, they may cause difficulties or even failures in pain relief, worsening or delaying the continuation of treatment planning and accomplishment.How to cite this articleTiradentes N, dos Santos ISS, de Vasconcelos RA, Balducci I, Kubo CH, Torres CRG, Almeida JD, Gomes APM. Patient Attendance for Emergency Care in a Brazilian Dental School. World J Dent 2012;3(1):11-17.NoteThis study was revised and approved by Local Ethics Committee (070/2006-PH/CEP).
Oral Health Status, Hygiene Habits and Treatment Needs among Elderly Brazilians: A Cross-sectional Study
Authors: Consani Rafael Leonardo Xediek , dos Santos Mateus Bertolini Fernandes , Montenegro Fernando Luiz Brunetti , Arcas Silvana Papaléo , Hiratsuka Marcel , Marchini Leonardo 
Published: May 2012

ABSTRACT

Purpose
Oral health status seems to play an important role in the quality of life of the elderly, the aim of this study was to verify oral hygiene habits, oral health status and treatment needs of a sample of elderly Brazilians in the city of São Paulo.

Materials and methods
A convenience sample of 167 individuals underwent interviews and clinical examinations.

Results
A total of 107 patients (64.1%) reported brushing their teeth 3 or more times per day, 42.5% floss their teeth, and 24.0% uses mouthwash. The average time since the patients’ last visit to a dentist was 2.2 years (±4.6). Only 24.6% of the teeth were periodontally healthy, and the most prevalent pathological condition was gingival recession (46.1%). Eighty-two percent use some type of dental prosthesis. A total of 35.3% of the sample required periodontal treatment, 32.9% needed direct restorations, 39.5% removable partial dentures and 23.4% complete dentures. Positive associations were found between the current condition of oral health and flossing (p = 0.004) and between the current condition and the use of interdental brushing (p = 0.009), and an inverse association with the time elapsed since the last dental appointment (p = 0.016).

Conclusion
The oral health status in this sample was better than observed in previous Brazilian surveys, but dental treatment needs remain high, mainly regarding prosthetic needs.

How to cite this article
dos Santos MBF, Montenegro FLB, Arcas SP, Hiratsuka M, Consani RLX, Marchini L. Oral Health Status, Hygiene Habits and Treatment Needs among Elderly Brazilians: A Cross-sectional Study. World J Dent 2012;3(1): 22-25.