bluuebunny/crossref_metadata_2025_split huggingface dataset:
Presence of a functional receptor for GLP‐1 in osteoblastic cells, independent of the cAMP‐linked GLP‐1 receptor
Authors: Nuche‐Berenguer Bernardo , Portal‐Núñez Sergio , Moreno Paola , González Nieves , Acitores Alicia , López‐Herradón Ana , Esbrit Pedro , Valverde Isabel , Villanueva‐Peñacarrillo María L. 
Published: May 2010

AbstractGlucagon‐like peptide 1 (GLP‐1) controls glucose metabolism in extrapancreatic tissues through receptors other than the pancreatic cAMP‐linked GLP‐1 receptor; also, GLP‐1 induces an insulin‐ and PTH‐independent bone anabolic action in insulin‐resistant and type‐2 diabetic rats. Here we searched for the presence and characteristics of GLP‐1 receptors in osteoblastic MC3T3‐E1 cells. [125I]‐GLP‐1 specific binding to MC3T3‐E1 cells was time‐ and temperature‐dependent, reaching maximal value at 30 min at 25°C; in these conditions, [125I]‐GLP‐1 binding was dissociable, and displaced by GLP‐1, partially by GLP‐2, but not by exendin‐4 (Ex‐4), exendin‐9 (Ex‐9), glucagon or insulin; Scatchard analysis of the unlabeled GLP‐1 data showed high and low affinity binding sites; cross‐linking of GLP‐1 binding revealed an estimated 70 kDa band, almost undetectable in the presence of 10−6 M GLP‐1. GLP‐1, Ex‐9, insulin or glucagon failed to modify cellular cAMP content, while GLP‐2 and Ex‐4 increased it. However, GLP‐1 induced an immediate hydrolysis of glycosylphosphatidylinositols (GPIs) generating short‐lived inositolphosphoglycans (IPGs), and an increase in phosphatidylinositol‐3 kinase (PI3K) and mitogen activated protein kinase (MAPK) activities; Ex‐4 also affected GPIs, but its action was delayed with respect to that of GLP‐1. This incretin was found to decrease Runx2 but increased osteocalcin gene expression, without affecting that of osteoprotegerin or the canonical Wnt pathway activity in MC3T3‐E1 cells which do not express the pancreatic GLP‐1 receptor. Our data demonstrate for the first time that GLP‐1 can directly and functionally interact with osteoblastic cells, possibly through a GPI/IPG‐coupled receptor. J. Cell. Physiol. 225: 585–592, 2010. © 2010 Wiley‐Liss, Inc.
1α,25‐(OH)2D3 acts in the early phase of osteoblast differentiation to enhance mineralization via accelerated production of mature matrix vesicles
Authors: Woeckel V.J. , Alves R.D.A.M. , Swagemakers S.M.A. , Eijken M. , Chiba H. , van der Eerden B.C.J. , van Leeuwen J.P.T.M. 
Published: May 2010

Abstract1α,25‐dihydroxyitamin D3 (1,25D3) deficiency leads to impaired bone mineralization. We used the human pre‐osteoblastic cell line SV‐HFO, which forms within 19 days of culture an extracellular matrix that starts to mineralize around day 12, to examine the mechanism by which 1,25D3 regulates osteoblasts and directly stimulates mineralization. Time phase studies showed that 1,25D3 treatment prior to the onset of mineralization, rather than during mineralization led to accelerated and enhanced mineralization. This is supported by the observation of unaltered stimulation by 1,25D3 even when osteoblasts were devitalized just prior to onset of mineralization and after 1,25D3 treatment. Gene Chip expression profiling identified the pre‐mineralization and mineralization phase as two strongly distinctive transcriptional periods with only 0.6% overlap of genes regulated by 1,25D3. In neither phase 1,25D3 significantly altered expression of extracellular matrix genes. 1,25D3 significantly accelerated the production of mature matrix vesicles (MVs) in the pre‐mineralization. Duration rather than timing determined the extent of the 1,25D3 effect. We propose the concept that besides indirect effects via intestinal calcium uptake 1,25D3 directly accelerates osteoblast‐mediated mineralization via increased production of mature MVs in the period prior to mineralization. The accelerated deposition of mature MVs leads to an earlier onset and higher rate of mineralization. These effects are independent of changes in extracellular matrix protein composition. These data on 1,25D3, mineralization, and MV biology add new insights into the role of 1,25D3 in bone metabolism and emphasize the importance of MVs in bone and maintaining bone health and strength by optimal mineralization status. J. Cell. Physiol. 225: 593–600, 2010. © 2010 Wiley‐Liss, Inc.
Voltage‐dependent κ‐opioid modulation of action potential waveform‐elicited calcium currents in neurohypophysial terminals
Authors: Velázquez‐Marrero Cristina M. , Marrero Héctor G. , Lemos José R. 
Published: May 2010

AbstractRelease of neurotransmitter is activated by the influx of calcium. Inhibition of Ca2+ channels results in less calcium influx into the terminals and presumably a reduction in transmitter release. In the neurohypophysis (NH), Ca2+ channel kinetics, and the associated Ca2+ influx, is primarily controlled by membrane voltage and can be modulated, in a voltage‐dependent manner, by G‐protein subunits interacting with voltage‐gated calcium channels (VGCCs). In this series of experiments we test whether the κ‐ and µ‐opioid inhibition of Ca2+ currents in NH terminals is voltage‐dependent. Voltage‐dependent relief of G‐protein inhibition of VGCC can be achieved with either a depolarizing square pre‐pulse or by action potential waveforms. Both protocols were tested in the presence and absence of opioid agonists targeting the κ‐ and µ‐receptors in neurohypophysial terminals. The κ‐opioid VGCC inhibition is relieved by such pre‐pulses, suggesting that this receptor is involved in a voltage‐dependent membrane delimited pathway. In contrast, µ‐opioid inhibition of VGCC is not relieved by such pre‐pulses, indicating a voltage‐independent diffusible second‐messenger signaling pathway. Furthermore, relief of κ‐opioid inhibition during a physiologic action potential (AP) burst stimulation indicates the possibility of activity‐dependent modulation in vivo. Differences in the facilitation of Ca2+ channels due to specific G‐protein modulation during a burst of APs may contribute to the fine‐tuning of Ca2+‐dependent neuropeptide release in other CNS terminals, as well. J. Cell. Physiol. 225: 223–232, 2010. © 2010 Wiley‐Liss, Inc.
Overexpression of fucosyltransferase IV promotes A431 cell proliferation through activating MAPK and PI3K/Akt signaling pathways
Authors: Yang Xue‐Song , Liu Shuai , Liu Yue‐Jian , Liu Ji‐Wei , Liu Ting‐Jiao , Wang Xiao‐Qi , Yan Qiu 
Published: May 2010

AbstractLewis Y (LeY) is a carbohydrate tumor‐asssociated antigen. The majority of cancer cells derived from epithelial tissue express LeY type difucosylated oligosaccharide. Fucosyltransferase IV (FUT4) is an essential enzyme that catalyzes the synthesis of LeY oligosaccharide. Our previous studies have shown that FUT4 overexpression promotes A431 cell proliferation, but the mechanism is still largely unknown. Herein, we investigated the role of the mitogen‐activated protein kinases (MAPKs) and phosphoinositide‐3 kinase (PI3K)/Akt signaling pathways on FUT4‐induced cell proliferation. Results show that overexpression of FUT4 increases the phosphorylation of ERK1/2, p38 MAPK, and PI3K/Akt. Inhibitors of PI3K (LY294002 and Wortmannin) prevented the phosphorylation of ERK1/2, p38 MAPK, and Akt PI3K). Moreover, phosphorylation of Akt is abolished by inhibitors of ERK1/2 (PD98059) and p38 MAPK (SB203580). These data suggested that FUT4 not only activates MAPK and PI3K/Akt signals, but also promotes the crosstalk among these signaling pathways. In addition, FUT4‐induced stimulation of cell proliferation correlates with increased cell cycle progression by promoting cells into S‐phase. The mechanism involves in increased expression of cyclin D1, cyclin E, CDK 2, CDK 4, and pRb, and decreased level of cyclin‐dependent kinases inhibitors p21 and p27, which are blocked by the inhibitors of upstream signal molecules, MAPK and PI3K/Akt. In conclusion, these studies suggest that FUT4 regulates A431 cell growth through controlling cell cycle progression via MAPK and PI3K/Akt signaling pathways. J. Cell. Physiol. 225: 612–619, 2010. © 2010 Wiley‐Liss, Inc.
The culture and transplantation of human limbal stem cells
Authors: Ahmad Sajjad , Osei‐Bempong Charles , Dana Reza , Jurkunas Ula 
Published: May 2010

AbstractThe cornea is the clear front of the eye and its surface is composed of an epithelium. This is renewed by stem cells located at the limbus, which encircles the periphery of the cornea. These limbal stem cells become lost or deficient in the blinding disease of limbal stem cell deficiency. In this review article, we discuss the historical perspective in managing limbal stem cell deficiency as well as describing the more contemporary treatment options, and in particular the culture and transplantation of human limbal stem cells. This treatment was first proposed 13 years ago and many case series have been presented to date showing promising outcomes of this technique. However, challenges still remain in treating the debilitating disease of limbal stem cell deficiency. Here we discuss some of the questions, which remain to be answered in this field. J. Cell. Physiol. 225: 15–19, 2010. © 2010 Wiley‐Liss, Inc.
B‐type natriuretic peptide and extracellular matrix protein interactions in human cardiac fibroblasts
Authors: Huntley Brenda K. , Ichiki Tomoko , Sangaralingham S. Jeson , Chen Horng H. , Burnett John C. 
Published: May 2010

AbstractCardiac fibroblasts (CFs) regulate myocardial remodeling by proliferating, differentiating, and secreting extracellular matrix (ECM) proteins. B‐type natriuretic peptide (BNP) is anti‐fibrotic, inhibits collagen production, augments matrix metalloproteinases, and suppresses CF proliferation. Recently, we demonstrated that the ECM protein fibronectin (FN) augmented production of BNP's second messenger, 3′, 5′ cyclic guanosine monophosphate (cGMP) in CFs, supporting crosstalk between FN, BNP, and its receptor, natriuretic peptide receptor A (NPR‐A). Here, we address the specificity of FN to augment cGMP generation by investigating other matrix proteins, including collagen IV which contains RGD motifs and collagen I and poly‐L‐lysine, which have no RGD domain. Collagen IV showed increased cGMP generation to BNP similar to FN. Collagen I and poly‐L‐lysine had no effect. As FN also interacts with integrins, we then examined the effect of integrin receptor antibody blockade on BNP‐mediated cGMP production. On FN plates, antibodies blocking RGD‐binding domains of several integrin subtypes had little effect, while a non‐RGD domain interfering integrin αvβ3 antibody augmented cGMP production. Further, on uncoated plates, integrin αvβ3 blockade continued to potentiate the BNP/cGMP response. These studies suggest that both RGD containing ECM proteins and integrins may interact with BNP/NPR‐A to modulate cGMP generation. J. Cell. Physiol. 225: 251–255, 2010. © 2010 Wiley‐Liss, Inc.
Link between high‐affinity adenosine concentrative nucleoside transporter‐2 (CNT2) and energy metabolism in intestinal and liver parenchymal cells
Authors: Huber‐Ruano Isabel , Pinilla‐Macua Itziar , Torres Gonzalo , Casado F. Javier , Pastor‐Anglada Marçal 
Published: May 2010

AbstractConcentrative nucleoside transporter 2 (CNT2) is a high‐affinity adenosine transporter that may play physiological roles beyond nucleoside salvage. Previous reports relate CNT2 function to modulation of purinergic signaling and energy metabolism in intestinal and liver parenchymal cells (Duflot et al., 2004, Mol Cell Biol 24:2710–2719; Aymerich et al., 2006, J Cell Sci 119:1612–1621). In the present study, to further examine the link between CNT2 and energy metabolism, CNT2 protein partners were identified using the bacterial two‐hybrid and GST pull‐down approaches. The N‐terminal segment of CNT2 was used as bait, since proteins lacking this domain display impaired plasma membrane insertion and intracellular retention. Glucose‐regulated protein 58 (GRP58) was identified as a potential rCNT2 partner in pull‐down experiments. Two‐hybrid screening performed against a liver human cDNA library led to the identification of aldolase B as another hCNT2 partner. Aldolase B‐RFP and endogenous GRP58 separately co‐localized with CNT2 in HeLa cells transfected with YFPrCNT2. CNT2 interaction with GRP58 was validated using co‐immunoprecipitation experiments. In HeLa cells, fluorescence resonance energy transfer (FRET) efficiency increased upon fructose addition, consistent with a transient interaction between aldolase B and the transporter. The physiological basis for in vivo interactions was derived from experiments in which GRP58 was inhibited or overexpressed and aldolase B activity stimulated towards glycolysis. GRP58 appeared to be a negative effector of CNT2 function, whereas aldolase B flux modulated CNT2 activity via a mechanism involving acquisition of higher affinity for its substrates. These findings support the theory that CNT2 plays roles other than salvage and establishes links with energy metabolism. J. Cell. Physiol. 225: 620–630, 2010. © 2010 Wiley‐Liss, Inc.
Late glacial to Holocene planktic foraminifera bioevents and climatic record in the South Adriatic Sea
Authors: Siani Giuseppe , Paterne Martine , Colin Christophe 
Published: May 2010

AbstractQuantitative analyses of planktic foraminifera, sea‐surface temperatures (SSTs), oxygen isotope and radiocarbon dating from a deep‐sea core recovered in the South Adriatic Sea have been used to reconstruct a subcentennial climatic and biochronological record since the late glacial (the last 24 cal. ka BP). The identification and relative abundance of 25 species of planktic foraminifera along the core have provided a continuous record of the faunal changes over this time interval. These results have permitted the establishment of 10 biozones in the South Adriatic Sea based on the appearance and/or disappearance of the main specific taxa, from peaks of abundance and/or by modification in marine conditions. The robust chronology of the South Adriatic core allowed correlation of SST estimates to the GISP2 ice core record, indicating that the main climate changes recorded in Greenland ice cores over the last 24 ka are recorded and globally synchronous with those observed in the South Adriatic Sea. This finding further allows comparison of the planktic foraminifera record with the event stratigraphic scheme proposed by the INTIMATE group in order to better identify the relationship between past climatic changes and the response of microfaunal assemblages in the South Adriatic. Copyright © 2010 John Wiley & Sons, Ltd.
Response of testate amoeba assemblages to environmental and climatic changes during the Lateglacial–Holocene transition at Lake Lautrey (Jura Mountains, eastern France)
Authors: Wall Adeline A. J. , Magny Michel , Mitchell Edward A. D. , Vannière Boris , Gilbert Daniel 
Published: May 2010

AbstractWe tested the response of lacustrine testate amoebae (thecamoebians) to climate and environmental changes for the Lateglacial–Holocene transition. The palaeoenvironmental history of the study site (Lake Lautrey, Jura Mountains, eastern France) was previously established based on high‐resolution multi‐proxy studies of the same core. The present study is characterised by a high taxonomic resolution (54 taxa), inclusion of small species (down to 25 µm) and high total counts (>500 individuals per sample on average). Changes in the composition of testate amoeba assemblages (dominant species and assemblage structure), as well as in the accumulation rate (tests cm−2 a−1), corresponded to major climatic phases (i.e. Oldest Dryas, Bølling–Allerød Interstadial, Younger Dryas, Preboreal) as well as changes in organic matter inputs. Furthermore, decreases in the accumulation rate characterised minor short‐lived cooling events, such as Older Dryas event or Gerzensee oscillation. However, the Preboreal oscillation, which was well registered by other proxies at Lake Lautrey, could not be recognised in the testate amoeba record. This work demonstrates that lacustrine testate amoebae can be used for palaeoclimatic and palaeoecological reconstructions. Nevertheless, a better understanding of the relation between climate, organic matter and lacustrine testate amoebae requires further high‐resolution studies based on multi‐proxy approaches and the development of appropriate modern analogues. Copyright © 2010 John Wiley & Sons, Ltd.
Chironomid‐inferred late‐glacial summer air temperatures from Lough Nadourcan, Co. Donegal, Ireland
Authors: Watson Jenny E. , Brooks Stephen J. , Whitehouse Nicki J. , Reimer Paula J. , Birks H. John B. , Turney Chris 
Published: May 2010

AbstractWestern Ireland, located adjacent to the North Atlantic, and with a strongly oceanic climate, is potentially sensitive to rapid and extreme climate change. We present the first high‐resolution chironomid‐inferred mean July temperature reconstruction for Ireland, spanning the late‐glacial and early Holocene (LGIT, 15–10 ka BP). The reconstruction suggests an initial rapid warming followed by a short cool phase early in the interstadial. During the interstadial there are oscillations in the inferred temperatures which may relate to Greenland Interstadial events GI‐1a–e. The temperature decrease into the stadial occurs in two stages. This two‐stage drop can also be seen in other late‐glacial chironomid‐inferred temperature records from the British Isles. A stepped rise in temperatures into the Holocene, consistent with present‐day temperatures in Donegal, is inferred. The results show strong similarities with previously published LGIT chironomid‐inferred temperature reconstructions, and with the NGRIP oxygen‐isotope curve, which indicates that the oscillations observed in the NGRIP record are of hemispherical significance. The results also highlight the influence of the North Atlantic on the Irish climate throughout the LGIT. Copyright © 2010 John Wiley & Sons, Ltd.
A forest history for the last 10 900 years at the site Storasjö, southern Sweden: implications from beetle assemblages
Authors: Olsson Fredrik , Lemdahl Geoffrey 
Published: May 2010

AbstractA continuous Holocene beetle record from southern Sweden is presented and compared with results from a previous study, at Stavsåkra. The comparison reveals both regional trends and local differences. The early Holocene, ca. 8900–7500 BC, was characterised by open, pine‐dominated woodlands at both sites. Fire and grazing seem to have maintained the open structure and favoured the established vegetation and fauna. During the middle Holocene, ca. 7500– 900 BC, the woodlands grew denser. There are no indications of grazing at the sites during this time period, whereas fires were frequent. The late Holocene, ca. 900 BC to the present, was characterised by a progressive opening up of the woodlands, mainly by land use such as grazing and clearance by fire. This process started ca. 2400 BC at Stavsåkra, where there were already settlements during the late Neolithic. At Storasjö, which probably was mainly used as outland until medieval times, the woodlands gradually opened up. Heather‐dominated heathland formed between ca. 900 and 800 BC at both sites. The two studies confirm that insect data contribute unique information concerning changes in structures and disturbances in forest ecosystems on long timescales. Copyright © 2010 John Wiley & Sons, Ltd.
Vegetation responses to climatic variability in the Swiss Southern Alps during the Misox event at the early–mid Holocene transition
Authors: Valsecchi Verushka , Tinner Willy 
Published: May 2010

AbstractSedimentary pollen, charcoal and plant macrofossil analyses with high resolution and precision suggest a strong shift in vegetation composition during the early to mid‐Holocene transition in the upper mountain belt. At Piano mire (1439 m above sea level (a.s.l.), Ticino, Switzerland) forests were dominated by Abies alba during the early Holocene (prior to ca. 8000 cal. a BP). Abrupt collapses of A. alba at ca. 7800–7400 cal. a BP enabled the expansion of the light‐demanding pioneer Betula. Afterwards A. alba populations regained their previous abundance in the forests. Within the dating uncertainties of our record we assume that a unique combination of wet and cold years between 8400 and 7500 cal. a BP led to repeated lethal disadvantages for Abies. Our record of Abies oscillations is in good biostratigraphic agreement with the record that has been used to define the Misox cold event (Pian di Signano, 1540 m a.s.l.), which has been previously correlated with the 8200 cal. a BP event. Given the age estimates of the Abies collapses in our well‐dated record, our results suggest that additional efforts are needed to understand the linkage between the Misox and the 8200 cal. a BP event. They imply a high sensitivity of mountain vegetation far below the tree line (∼800 m) to Holocene climatic changes of about 2°C in annual air temperature. Copyright © 2010 John Wiley & Sons, Ltd.
Differential gene expression profile of MAGE family in taiwanese patients with colorectal cancer
Authors: Chung Fu‐Yen , Cheng Tian‐Lu , Chang Hui‐Jen , Chiu Hua‐Hsien , Huang Ming‐Yii , Chang Ming‐Sung , Chen Chung‐Chi , Yang Ming‐Je , Wang Jaw‐Yuan , Lin Shiu‐Ru 
Published: May 2010

AbstractBackgroundThe melanoma‐associated antigen (MAGE) gene family consists of different expression patterns in various tumor types. They are considered tumor‐specific antigens and are ideal targets for cancer immunotherapy. The purpose of this study is to identify the expression profiles of the MAGE family genes in Taiwanese colorectal cancer patients.MethodsIn this study, a well‐constructed chip array platform was used to analyze the expression of the MAGE family genes of 100 colorectal cancer tissues. Statistical analysis of the experimental results and patients' clinical manifestations were also conducted.ResultsThe results showed MAGE‐A2 (87%), ‐A7 (83%), ‐A8 (75%), ‐A12 (71%), ‐B2 (75%), ‐B3 (79%), ‐D2 (75%), ‐F1 (79%), and ‐H1 (70%) were significantly overexpressed genes in colorectal cancer tissues. MAGE‐A2 was the most highly overexpressed gene among the MAGE family. MAGE‐B3 gene expression is statistically correlated with tumor size, lymph node, and UICC stage. In addition, the overexpression of MAGE‐D2 and ‐H1 genes are statistically correlated to the tumor size and depth, respectively (P < 0.05).ConclusionsThis is the first comprehensive report to clarify the differential expression profile of whole MAGE family in CRCs, and it might provide some crucial information about the carcinogenesis and progression in Taiwanese patients with CRC. J. Surg. Oncol. 2010;102:148–153. © 2010 Wiley‐Liss, Inc.