bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stability of Ethanol in Blood and Urine Samples
Authors: Mandić-Radić Slavka , Džingalašević Gordana , Luković Nevena 
Published: December 2009

Stability of Ethanol in Blood and Urine SamplesThe changes of ethanol concentrations in whole blood and urine samples were analyzed depending on temperatures and duration of storage. The aim of the study was to establish standards for the Institute laboratory. Samples of whole blood and urine, taken from drivers with excessive alcohol concentrations (6 groups, 15 samples per each), were analyzed upon delivery and then after storage during different time intervals and at different temperatures. The results showed that alcohol concentrations were significantly reduced with the increase of temperature and prolongation of storage. Only the whole blood samples stored for up to one month at -20 °C did not show significant changes. Room temperature storage of samples is the least suitable way of keeping them, independently of the duration of storage. Urines are not less reliable samples than blood. There are no ethanol differences between blood samples with and without sodium fluoride.
Wrong-site craniotomy: analysis of 35 cases and systems for prevention
Authors: Cohen Fred. L. , Mendelsohn Daniel , Bernstein Mark 
Published: December 2009

Object
The purpose of this case review was to identify and analyze existing wrong-site craniotomy (WSC) cases to determine the factors that contributed to the errors and to suggest preventative strategies for WSC. Wrong-site surgery (WSS) is a devastating surgical error that has gained increased public attention in recent years due to some high-profile cases. Despite the implementation of preventative methods such as preoperative checklists and surgical time-outs, WSS still occurs to this day. The clinical consequences of WSC are distinct compared with other types of WSS due to the unique function of the brain.

Methods
The authors searched medical, legal, and media databases and contacted state medical licensing boards to identify and gather information about WSC cases. The cases were reviewed and analyzed for factors that contributed to the errors.

Results
Four major categories of contributing factors were found: 1) communication breakdown; 2) inadequate preoperative checks; 3) technical factors and imaging; and 4) human error. The WSC cases are used to illustrate how these types of factors can precipitate the surgical error. Clinical outcomes and disciplinary actions are summarized. Obtaining information about the cases discovered was very challenging, in part because WSS reporting is inadequate.

Conclusions
This case review demonstrates that a broad range of events and factors can cause human errors to breach patient safeguards and lead to a WSC; however, in essentially all cases the WSCs were preventable with strict adherence to comprehensive and thorough protocols.
Poor drug distribution as a possible explanation for the results of the PRECISE trial
Authors: Sampson John H. , Archer Gary , Pedain Christoph , Wembacher-Schröder Eva , Westphal Manfred , Kunwar Sandeep , Vogelbaum Michael A. , Coan April , Herndon James E. , Raghavan Raghu , Brady Martin L. , Reardon David A. , Friedman Allan H. , Friedman Henry S. , Rodríguez-Ponce M. Inmaculada , Chang Susan M. , Mittermeyer Stephan , Croteau David , Puri Raj K. , _ _ 
Published: December 2009

Object
Convection-enhanced delivery (CED) is a novel intracerebral drug delivery technique with considerable promise for delivering therapeutic agents throughout the CNS. Despite this promise, Phase III clinical trials employing CED have failed to meet clinical end points. Although this may be due to inactive agents or a failure to rigorously validate drug targets, the authors have previously demonstrated that catheter positioning plays a major role in drug distribution using this technique. The purpose of the present work was to retrospectively analyze the expected drug distribution based on catheter positioning data available from the CED arm of the PRECISE trial.

Methods
Data on catheter positioning from all patients randomized to the CED arm of the PRECISE trial were available for analyses. BrainLAB iPlan Flow software was used to estimate the expected drug distribution.

Results
Only 49.8% of catheters met all positioning criteria. Still, catheter positioning score (hazard ratio 0.93, p = 0.043) and the number of optimally positioned catheters (hazard ratio 0.72, p = 0.038) had a significant effect on progression-free survival. Estimated coverage of relevant target volumes was low, however, with only 20.1% of the 2-cm penumbra surrounding the resection cavity covered on average. Although tumor location and resection cavity volume had no effect on coverage volume, estimations of drug delivery to relevant target volumes did correlate well with catheter score (p < 0.003), and optimally positioned catheters had larger coverage volumes (p < 0.002). Only overall survival (p = 0.006) was higher for investigators considered experienced after adjusting for patient age and Karnofsky Performance Scale score.

Conclusions
The potential efficacy of drugs delivered by CED may be severely constrained by ineffective delivery in many patients. Routine use of software algorithms and alternative catheter designs and infusion parameters may improve the efficacy of drugs delivered by CED.
Natural History of Cervical Papilloma Virus Infection in Systemic Lupus Erythematosus — A Prospective Cohort Study
Authors: TAM LAI-SHAN , CHAN PAUL K.S. , HO SUZANNE C. , YU MAY M.Y. , YIM SO-FAN , CHEUNG TAK-HONG , WONG MARTIN C.S. , LI EDMUND K. 
Published: December 2009

Objective.To ascertain the incidence, cumulative prevalence, persistence, and clearance of human papilloma virus (HPV) infection in patients with systemic lupus erythematosus (SLE), and to assess the risk factors for the acquisition and persistence of HPV infection.Methods.One hundred forty-four patients with SLE were evaluated at 6-month intervals for up to 3 years. At each visit, a Pap test, a test for HPV DNA, and clinical assessment were performed.Results.The cumulative prevalence of HPV infection increased significantly (12.5% at baseline to 25.0% after 3 years; p = 0.006). Regarding type-specific HPV infection, 18.8% patients experienced 68 incident infections. The cumulative prevalence of high-risk HPV infection (11.1% at baseline to 20.8% after 3 years; p = 0.02) and multiple HPV infection also increased significantly (6.9% at baseline to 16.7% after 3 years; p = 0.009). Half (33/68, 48.5%) of the incident infections persisted for ≥ 6 months. Overall, 29/32 (90.6%) of the preexisting infection and 10/68 (14.7%) of the incident infections were cleared. Independent risk factors associated with incident HPV infection included younger age at first sexual intercourse (p = 0.025) and baseline Systemic Lupus International Collaborating Clinics score ≥ 1 (p = 0.038). Independent risk factor associated with persistent HPV infection included preexisting HPV infection (p = 0.04) and multiple HPV infection during first incident infection (p = 0.02).Conclusion.High frequency of persistent HPV infection, especially high-risk and multiple HPV infection, may explain why squamous intraepithelial lesions occurred frequently in patients with SLE. Patients with high inflammatory burden are at risk of acquiring HPV infection.
Suitability of Hammond's method for determining landform units in Slovenia
Authors: Hrvatin Mauro , Perko Drago 
Published: December 2009

Landform is often the most important factor in distinguishing between regions and an important element of geographic classification, typification, and regionalization; this is why morphological classification has had a long tradition in Slovenia and abroad. One of the best-known classifications was developed by the American geographer Edwin H. Hammond, who classified the landforms of the United States in great detail. Later on, his method was applied several times using a geographic information system and digital elevation model. Computer land-surface classification became more objective, whereas the selection of classification elements and their classes remained subjective. Hammond’s method of determining landform units is known throughout the world and this is why it has also been tested in Slovenia. First, the original classification elements were taken into account and only thirteen units of the twenty-one landform units specified by Hammond were selected. Due to weaknesses that were revealed, Hammond’s original method was suitably adapted: the form and size of the basic window and the boundaries between classification element classes were changed. Nineteen landform units were thus identified in Slovenia using the adapted method.
How Modern Decline Analysis and Material Balance Technique Can Be Used To Answer One of The Most Important Questions.... Are We Leaving Oil Behind? : Case Study
Authors: Alonso Hector Gomez , Kharusi Majida , Yeo Phyllis Niam Chay , German Mike 
Published: December 2009

Abstract
This paper presents the results from Modern Decline Analysis and Material Balance study in a mature Field, in the Sabah State of the region of Malaysia. The study represents the first part of ongoing subsurface evaluations. The identification of non-drained areas using Modern Decline Analysis together with the use of Material Balance has led successfully not only to evaluate the potential but also to successfully determining the viability of infill well locations.
Prior to obtaining a clear understanding of the subsurface drive mechanisms and production data, reservoir simulation was used to establish reservoir potential and carry out infill drilling evaluations. The results were highly variable and optimistic in some cases, and somewhat disappointing in others. Subsequent well results indicated large discrepancies between the static models and the actual field results. Once these discrepancies had been reconciled, a consistent understanding of the reservoir performance began to develop.
This paper presents the results of applying modern decline analysis and material balance techniques to different reservoirs, starting from the basic steps of quality assurance and quality review of the entire production data, in order to incorporate them together with the pressure of the neighbouring wells to the future infill target. Each reservoir was matched to the observed pressure, for both techniques Modern Decline Analysis and Material Balance, which again showed signs of nondrained areas, secondary support mechanism and infill opportunities. Modern Decline Analysis was carried out for each of the wells and from the results, a map of Contacted oil Volumes and Recovery Factor was generated for each of the reservoirs, where zones of high oil contacted volumes and low recovery factors were identified. Later Material Balance was applied to assess on the energy levels of the non-drained areas. As a result, several optimization and infill opportunities were identified.
Prostaglandin E2 Exerts Homeostatic Regulation of Pulmonary Vascular Remodeling in Allergic Airway Inflammation
Authors: Lundequist Anders , Nallamshetty Samridhi N. , Xing Wei , Feng Chunli , Laidlaw Tanya M. , Uematsu Satoshi , Akira Shizuo , Boyce Joshua A. 
Published: December 2009

Abstract
Nonselective inhibition of PG synthesis augments inflammation in mouse models of airway disease, but the roles of individual PGs are not completely clarified. To investigate the role of PGE2 in a mouse model of airway inflammation induced by a natural allergen, we used mice lacking the critical terminal synthetic enzyme, microsomal PGE2 synthase (mPGES)-1. Mice lacking mPGES-1 (ptges−/− mice) and wild-type C57BL/6 controls were challenged intranasally with low doses of an extract derived from the house dust mite Dermatophagoides farinae (Der f). The levels of PGE2 in the bronchoalveolar lavage fluids of Der f-treated ptges−/− mice were ∼80% lower than the levels in wild-type controls. Der f-induced bronchovascular eosinophilia was modestly enhanced in the ptges−/− mice. Both Der f-treated strains showed similar increases in serum IgE and IgG1, as well as comparable levels of Th1, Th2, and Th17 cytokine production by Der f-stimulated spleen cells. These findings indicated that mPGES-1–derived PGE2 was not required for allergen sensitization or development of effector T cell responses. Unexpectedly, the numbers of vascular smooth muscle cells and the thickness of intrapulmonary vessels were both markedly increased in the Der f-treated ptges−/− mice. These vascular changes were suppressed by the administration of the stable PGE2 analog 16, 16-dimethyl PGE2, or of selective agonists of the E-prostanoid (EP) 1, EP2, and EP3 receptors, respectively, for PGE2. Thus, mPGES-1 and its product, PGE2, protect the pulmonary vasculature from remodeling during allergen-induced pulmonary inflammation, and these effects may be mediated by more than one EP receptor.
New developments in multiple sclerosis and pain management
Authors: Laundon Kerry 
Published: December 2009

AbstractPromising studies were reported at the 25th congress of the European Committee for Treatment and Research in MS (ECTRIMS), held on 9‐12 September 2009 in Düsseldorf, Germany, while data on a new treatment for chronic low back pain was presented at the 6th Triennial congress of the European Federation of Chapters (EFIC) of the International Association for the Study of Pain (IASP), held on 9‐12 September 2009 in Lisbon, Portugal. Copyright © 2009 John Wiley & Sons, Ltd.
A transmission electron microscope study on the route of entry of triclabendazole into the liver fluke,Fasciola hepatica
Authors: TONER E. , BRENNAN G. P. , McCONVERY F. , MEANEY M. , FAIRWEATHER I. 
Published: December 2009

SUMMARYUptake of triclabendazole by the liver fluke,Fasciola hepaticahas been studied by experiments designed to block either oral uptake of drug, by use of ligatures, or trans-tegumental diffusion, by allowing the drug to bind to an excess of bovine serum albumin (BSA) in the medium. Changes to the tegumental system, musculature and gut were assessed using transmission electron microscopy. Flukes were incubatedin vitrofor 24 h in TCBZ.SO (15 μg/ml). Disruption to the tegument and muscle was similar in ligatured and non-ligatured flukes, suggesting that closing the oral route did not affect drug uptake. The ultrastructure of the gastrodermal cells remained unchanged. Non-ligatured flukes were also incubated for 24 hin vitroin TCBZ.SO (15 μg/ml) in the presence of red blood cells (RBCs). Oral uptake of blood was demonstrated, but gut ultrastructure remained normal, whereas the tegument was severely disrupted. In separate experiments, ligatured and non-ligatured flukes were incubated in TCBZ.SO (15 μg/ml) in the presence of BSA (30 mg/ml) for 24 hin vitro. There was a marked decrease in the degree of tegumental disruption observed compared with TCBZ.SO action alone; again, the gut remained normal. The findings support previous morphological and pharmacological studies indicating that trans-tegumental uptake of triclabendazole predominates in the liver fluke.
Language policy for China’s minorities
Authors: Bradley David 
Published: December 2009

The Yi national minority of southwestern China has four distinct orthographic traditions: Nosu, Nasu, Nisu and Sani. All are based on traditional systems using the same logographic principle as Chinese writing and a few Chinese characters. Otherwise, they are very different, both from Chinese and to a lesser degree from each other.	Since 1950, orthographic reform has taken place separately in the three main provinces where Ngwi or Yi languages are spoken. This process has been a top-down language planning effort, run by leaders and scholars from within the various Yi communities of each province. In addition, for local and scholarly purposes, the traditional scripts continue in use.	This paper discusses and exemplifies the traditional and new orthographic systems and the process of reform which created three modern orthographies alongside the four traditional ones which also continue in limited use. The top-down process of script reform is parallel to other areas of script, language and other policy developments in China since 1950, with a centralised model that achieves some great successes and some failures, and going through a series of major changes affecting the way the languages of the Yi are written.
Response of nematode communities after large‐scale ice‐shelf collapse events in the Antarctic Larsen area
Authors: RAES M. , ROSE A. , VANREUSEL A. 
Published: December 2009

AbstractOwing to large‐scale ice‐shelf disintegration events, the Antarctic Larsen A and B areas recently became ice‐free. During the ANT‐XXIII/8 Polarstern campaign, this region was sampled for the first time. Our study is the first to investigate benthic communities in this area and their response to the collapse of ice shelves in the Antarctic. The nematofauna appears to be strongly influenced by the sudden ice‐cover removal, although its response differs from that of the macro‐ and megabenthos. Our results indicate that precollapse, sub‐ice communities were impoverished and characterized by low densities, low diversity and high dominance of a few taxa. This might still be visible at a station located deep inside the Larsen B embayment, where Halomonhystera was dominant. Post‐collapse recolonization of the ‘inner’ stations, i.e. those located furthermost from the former ice‐shelf edge, is believed to be a long‐time process. At the time of sampling, community structure at the inner stations was not or only slightly influenced by colonization, and might be structured by local environmental conditions. Our results indicate that a locally increased food supply after ice‐cover removal could provoke a faster, local response of the nematode assemblages compared with the response due to recolonization. Thalassomonhystera is recognized as an opportunist, taking advantage of increased food supply at inner stations A_South and B_North. Communities living close to the former ice‐shelf edge are believed to be at an intermediate or late stage of succession, with a dominance of Microlaimus, a common Antarctic genus and quick colonizer. Densities here were comparable with those at other Antarctic stations, whereas they were considerably decreased at the inner stations. In general, the collapse of the Larsen ice shelves initially has a positive effect on the shelf nematode fauna in the area, both in terms of abundance and diversity.
Guerrilla Employees: Should Managers Nurture, Tolerate, or Terminate Them?
Authors: O'Leary Rosemary 
Published: December 2009

“Guerrilla government” is Rosemary O'Leary's term for the actions of career public servants who work against the wishes—either implicitly or explicitly communicated—of their superiors. This form of dissent is usually carried out by those who are dissatisfied with the actions of public organizations, programs, or people, but typically, for strategic reasons, choose not to go public with their concerns in whole or in part. Rather than acting openly, guerrillas often move clandestinely behind the scenes, salmon swimming against the current of power. Guerrillas run the spectrum from anti‐establishment liberals to fundamentalist conservatives, from constructive contributors to deviant destroyers. Three public managers with significant experience comment on O'Leary's thesis that guerrilla government is about the power of career bureaucrats; the tensions between career bureaucrats and political appointees; organization culture; and what it means to act responsibly, ethically, and with integrity as a public servant. Karl Sleight, former director of the New York State Ethics Commission; David Warm, executive director of the Mid‐America Regional Council of Greater Kansas City; and Ralph R, Bauer, former deputy regional administrator of the U.S. Environmental Protection Agency in the Seattle and Chicago regions, present unique perspectives on the “guerrilla” influence on policy and management, as well as the challenges posed by this ever‐present public management phenomenon.
Guerrilla: One who engages in irregular warfare especially as a member of an independent unit. —Webster's New College Dictionary, 2008
The Pracademic and the Fed: The Leadership of Chairman Benjamin Bernanke
Authors: Khademian Anne M. 
Published: December 2009

When President Barack Obama nominated Ben Bernanke for a second term as chair of the Federal Reserve Board in August 2009, he emphasized Bernanke's “bold action and outside‐the‐box thinking” in preventing the recent financial collapse from turning into another Great Depression. While many saw this renomination as providing monetary policy stability and necessary institutional continuity during the crisis, Bernanke's “bold action” has generated robust criticism, mainly because Bernanke initiated fundamental reforms of traditional Fed practices. How has Bernanke's leadership style at the Fed evolved as the financial crisis of 2008–2009 has unfolded? Did his prior academic experience influence his decisions? What are the long‐term implications of Bernanke's innovative strategies for the Federal Reserve as an independent, powerful, policy making institution, traditionally one that possesses tremendous financial and political autonomy? If you want to build a factory, or fix a motorcycle, or set a nation right without getting stuck, then classical, structured, dualistic subject‐object knowledge, although necessary, isn't enough. You have to have some feeling for the quality of the work. You have to have a sense of what's good. That is what carries you forward … It's not just “intuition,” or unexplainable “skill” or “talent.” It's the direct result of contact with basic reality … —Robert M. Pirsig, Zen and the Art of Motorcycle Maintenance There are no atheists in foxholes and no ideologues in financial crises. —Benjamin Bernanke, 20091
Vaccinia Virus Particles Mix Inefficiently, and in a Way That Would Restrict Viral Recombination, in Coinfected Cells
Authors: Lin Y.-C. James , Evans D. H. 
Published: December 2009

ABSTRACT

It is well established that poxviruses are subjected to genetic recombination, but attempts to map vaccinia virus genes using classical genetic crosses were historically confounded by high levels of experimental noise and a poor correlation between physical and genetic map distances. These virus-by-virus crosses also never produced the 50% recombinant progeny that should be seen in experiments involving distant markers. Poxviruses replicate in membrane-wrapped cytoplasmic structures called virosomes (or factories) and we have developed a method for tracking the development of these structures using live cell imaging and cells expressing phage lambda Cro protein fused to enhanced green fluorescent protein (EGFP). The EGFP-cro protein binds nonspecifically to DNA and permits live cell imaging of developing vaccinia virus factories. Using this method, we see virosomes first appearing about 4 to 5 h postinfection. The early virosomes exhibit a compact appearance and then, after a period of exponential growth lasting several hours, blur and start to dissipate in a process presumably linked to viral packaging. During the growth period, the virosomes migrate toward the nuclear periphery while colliding and fusing at a rate dependent upon the numbers of infecting particles. However, even at high multiplicities of infection (10 PFU/cell), we estimate ∼20% of the virosomes never fuse. We have also used fluorescence
in situ
hybridization (FISH) methods to study virosomes formed by the fusion of viruses carrying different gene markers. FISH showed that DNA mixes rather poorly within fused virosomes and the amount of mixing is inversely dependent on the time between virosome appearance and fusion. Our studies suggest that the intracellular movement and mixing of virosomes create constraints that reduce opportunities for forming recombinants and that these phenomena create outcomes reflected in classical poxvirus genetics.