bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tumour suppressor gene TP53 mutations in atypical vascular lesions of breast skin following radiotherapy
Authors: Santi Raffaella , Cetica Valentina , Franchi Alessandro , Pepi Monica , Cesinaro Anna M , Miracco Clelia , Paglierani Milena , De Giorgi Vincenzo , Delfino Chiara , Difonzo Elisa M , Pimpinelli Nicola , Bianchi Simonetta , Sardi Iacopo , Santucci Marco , Massi Daniela 
Published: February 2011

Santi R, Cetica V, Franchi A, Pepi M, Cesinaro A M, Miracco C, Paglierani M, De Giorgi V, Delfino C, Difonzo E M, Pimpinelli N, Bianchi S, Sardi I, Santucci M & Massi D (2011) Histopathology58, 455–466 Tumour suppressor gene TP53 mutations in atypical vascular lesions of breast skin following radiotherapyAims:  Atypical vascular lesions (AVL) occurring at the site of radiotherapy represent an uncommon but well‐documented complication in the setting of breast‐conserving therapy for breast carcinoma. Although the biological behaviour of AVL has been regarded as benign, it has been suggested that AVL may represent a precursor of angiosarcoma. A better understanding of the biology of AVL is essential in order to assess appropriate patient management. The aim of the present study was to investigate alterations of tumour suppressor gene TP53 in a series of radiation‐induced AVL and angiosarcomas (AS).Methods and results:  Direct sequencing analysis of the TP53 gene showed the presence of at least one variation in 10 of 12 (83.3%) AVL and in seven of eight (87.5%) AS. The most common alteration in both categories was the P72R polymorphism in exon 4. One angiosarcoma sample carried a pathogenetically relevant disruptive mutation c.592delG, a frameshift deletion in exon 6, causing a premature stop codon.Conclusions:  The presence of TP53 alterations suggests that its mutational inactivation may be implicated in the pathogenesis of radiation‐associated vascular proliferations. The common mutational pathway suggested by our data supports the hypothesis that AVL and AS are biologically related entities, most probably representing the extremes of a morphological continuum.
Cord blood monocyte subsets are similar to adult and show potent peptidoglycan‐stimulated cytokine responses
Authors: Sohlberg Ebba , Saghafian‐Hedengren Shanie , Bremme Katarina , Sverremark‐Ekström Eva 
Published: February 2011

SummaryHuman monocytes can be divided into two major subpopulations, CD14++ CD16− and CD14+ CD16+ cells, which are suggested to play different roles in antimicrobial responses. In neonates, characteristics and functional responses of monocyte subsets have not previously been explored, and might contribute to the qualitative difference between neonatal and adult cytokine profiles. We report that at baseline, monocyte subsets in cord blood and adult peripheral blood are present in similar frequencies, and show similar expression of CD11c, CD80/CD86, CD163 and HLA‐DR. In response to the bacterial ligand peptidoglycan, cord blood monocytes had high inherent capacity for production of the early‐response cytokines with levels of tumour necrosis factor and interleukin‐12p70 exceeding adult levels, and also a higher phosphorylation of p38‐mitogen‐activated protein kinase. The CD14+ CD16+ cells expressed more interleukin‐12p70 than CD14++ CD16− cells and were present in a higher frequency in peptidoglycan‐stimulated cord blood mononuclear cell cultures. Together, the behaviour of cord blood CD14+ CD16+ cells following peptidoglycan stimulation might indicate a qualitative difference between the neonatal antimicrobial response and that of the adult. In addition we found that serum factors in cord blood and adult sera affected cytokine production similarly, with the exception of tumour necrosis factor, regardless of the source of serum or cells. Overall, our data provide new insights into monocyte heterogeneity in cord blood and monocyte subset responses to a bacterial ligand at birth.
Care recipients’ and family members’ perceptions of quality of older people care: a comparison of home‐based care and nursing homes
Authors: Hasson Henna , Arnetz Judith E 
Published: February 2011

Aims.  To compare care recipients’ and their relatives’ perceptions of quality of care in nursing homes and home‐based care.Background.  Older people care is increasingly being provided in community care facilities and private homes instead of hospitals. A few studies have compared care recipients’ and relatives’ perceptions of care quality in nursing homes as opposed to home‐based care.Design.  Cross‐sectional surveys.Methods.  Care recipients’ and relatives’ perceptions of quality of care were measured by questionnaire in 2003 in two older people care organisations. Quality measures were compared between care settings. Multiple regression was used to determine the predictors of overall quality ratings for each group, and possible interactions between quality measures and care settings were tested.Results.  Care recipients rated their opportunities for activities significantly lower in home care than in nursing homes. Relatives of care recipients in home‐based care rated several aspects of care quality significantly lower than relatives of nursing home residents. No significant interaction effects regarding predictors of overall quality ratings between the care settings were found. Staff behaviour was the strongest predictor of care recipients’ overall quality rating, and staff professional skills were the strongest predictor of relatives’ overall quality rating.Conclusions.  Compared with nursing homes, home‐based older people care seems to be in greater need of development regarding staff competence, staff interaction with relatives and activities offered to older people. In both settings, nursing staff behaviour influences these stakeholders’ satisfaction with care.Relevance to clinical practice.  These results point to a need to improve services in both care settings, but especially in home‐based care. It is suggested that care recipients’ preferences for social and physical activities be investigated on a regular basis in both care settings. Efforts should also be made to improve communication and interaction between family members and older people care staff, especially in home‐based care.
Pharmacokinetics of ceftiofur crystalline‐free acid sterile suspension in the equine
Authors: COLLARD W. T. , COX S. R. , LESMAN S. P. , GROVER G. S. , BOUCHER J. F. , HALLBERG J. W. , ROBINSON J. A. , BROWN S. A. 
Published: February 2011

Collard, W. T., Cox, S. R., Lesman, S. P., Grover, G. S., Boucher, J. F., Hallberg, J. W., Robinson, J. A., Brown, S. A. Pharmacokinetics of ceftiofur crystalline‐free acid sterile suspension in the equine. J. vet. Pharmacol. Therap.34, 476–481.Absolute bioavailability and dose proportionality studies were performed with ceftiofur in horses. In the absolute bioavailability study, thirty animals received either an intravenous dose of ceftiofur sodium at 1.0 mg/kg or an intramuscular (i.m.) dose of ceftiofur crystalline‐free acid (CCFA) at 6.6 mg/kg. In the dose proportionality study, 48 animals received daily i.m. ceftiofur sodium injections at 1.0 mg/kg for ten doses or two doses of CCFA separated by 96 h, with CCFA doses of 3.3, 6.6, or 13.2 mg/kg. Noncompartmental and mixed‐effect modeling procedures were used to assess pharmacokinetics (PK). CCFA was well absorbed with a bioavailability of 100%. AUC0–∞ and Cmax increased in a dose‐related manner following administration of the two doses of CCFA at 3.3, 6.6, and 13.2 mg/kg. The least‐squares mean terminal half‐life (t½) following the tenth daily i.m. injection of ceftiofur sodium at 2.2 mg/kg was 40.8 h, but the least‐squares mean t½ following the second i.m. injection of CCFA at 6.6 mg/kg was 100 h. The time that plasma ceftiofur equivalent concentrations remain above a threshold concentration of 0.2 μg/mL has been associated with efficacy, and following administration of two 6.6 mg/kg doses of CCFA, the mean time above 0.2 μg/mL was 262 h. Simulations with the nonlinear mixed‐effect PK model predicted that more than 97.5% of horses will have plasma ceftiofur equivalent concentrations >0.2 μg/mL for 96 h after the second 6.6 mg/kg dose of CCFA.
Subsistence and Sustainability in Post‐industrial Europe: The Politics of Small‐scale Farming in Europeanising Lithuania
Authors: Mincyte Diana 
Published: February 2011

AbstractThis article examines the marginalisation of small‐scale semi‐subsistence farming in the context of the European Union's sustainable development reforms as implemented in the new member countries. In documenting how small‐scale farming in post‐socialist Lithuania has been redefined from being a solution to the environmental and social degradation of industrialised agriculture under socialism to becoming a major obstacle in building sustainable agriculture, this case study offers a critique of sustainability as a developmental project and the asymmetrical relationships implicit in the rural development politics in Europe. Taking a historical‐comparative approach, this study demonstrates that the notion of sustainability has been built on assumptions about the industrialisation and globalisation of agri‐food systems that exclude alternative local forms of production, consumption and distribution. As a result, the implementation of such sustainable development policies leads to the reproduction of the industrialised agriculture and the exclusion of small‐scale farmers from the vision of sustainable rural societies.
The Influence of Urban Street Characteristics on Pedestrian Heat Comfort Levels in Philadelphia
Authors: Oka Masayoshi 
Published: February 2011

AbstractThis article demonstrates a way to model the urban microclimate using a combination of statistical analysis and a geographic information system (GIS). Field measurements of ambient temperature and relative humidity were collected in various urban settings at ground level over two surface materials and at two different times of the day. A panel autoregressive (PAR) model was created to conduct a regression analysis capable of accounting for the spatial autocorrelation. With the PAR analyses, selected urban street characteristics showed varying effects over the different surface materials and at different times of the day. The overall influences were consistent with previous findings. PAR models were then applied in GIS to create thermal prediction maps for the study area to determine the hot and cool spots. Since GIS is the most commonly used technology among a wide range of researchers and professionals, this article provides a means to improve the synthesis, integration, and sharing of information to understand the relationship between the heat‐vulnerable population and heat stress within the urban environment. This methodology will be useful in the effort to reduce the heat‐related morbidity and mortality, which are expected to increase with projected global warming.
Evidence‐Based Guidelines for the Care and Maintenance of Complete Dentures: A Publication of the American College of Prosthodontists
Authors: Felton David , Cooper Lyndon , Duqum Ibrahim , Minsley Glenn , Guckes Albert , Haug Steven , Meredith Patricia , Solie Caryn , Avery David , Deal Chandler Nancy 
Published: February 2011

AbstractThe current rates of edentulism have been estimated to be between 7% and 69% of the adult population internationally. In the United States, while the incidence of edentulism continues to decline, rapid population growth coupled with current economic conditions suggest that edentulism and conventional denture use will continue at current or higher numbers. Unfortunately, evidence‐based guidelines for the care and maintenance of removable complete denture prostheses do not exist. In 2009, the American College of Prosthodontists (ACP) formed a task force to establish evidence‐based guidelines for the care and maintenance of complete dentures. The task force comprised members of the ACP, the Academy of General Dentistry, American Dental Association (ADA) Council on Scientific Affairs, the American Dental Hygienists' Association, the National Association of Dental Laboratories, and representatives from GlaxoSmithKline Consumer Healthcare. The review process included the assessment of over 300 abstracts and selection of over 100 articles meeting inclusion criteria of this review. The task force reviewed synopses of the literature and formulated 15 evidence‐based guidelines for denture care and maintenance. These guidelines were reviewed by clinical experts from the participating organizations and were published in February 2011 issue of The Journal of the American Dental Association for widespread distribution to the dental community. These guidelines reflect the views of the task force.
Proresolving lipid mediators: potential for prevention and treatment of periodontitis
Authors: Van Dyke Thomas E. 
Published: February 2011

Van Dyke TE. Proresolving lipid mediators: potential for prevention and treatment of periodontitis. J Clin Periodontol 2011; 38 (Suppl. 11): 119–125. doi: 10.1111/j.1600‐051X.2010.01662.x. AbstractAim: Periodontitis is an inflammatory disease initiated by microbial biofilm. The host response to the biofilm destroys the periodontium mediated by an overly robust inflammatory response in susceptible individuals. Whether the excessive host response is genetic, epigenetic or mediated by environment is unknown. New pathways of resolution of inflammation have been discovered. Resolution of inflammation is an active, agonist‐mediated, programmed return to tissue homeostasis.Materials and Methods: Various computer‐based search engines were employed to identify papers relevant to resolution of inflammation.Results: Recent data suggest that chronic inflammatory periodontal disease may be a failure of resolution pathways as well as overexpression of proinflammatory pathways. In this review, the biology of resolution of inflammation will be examined in normal tissues and periodontal disease. Anti‐inflammatory pharmacologic agents [non‐steroidal anti‐inflammatory drugs (NSAIDs)] have been shown to prevent and slow the progression of periodontitis in animals and humans. However, the side effect profile of NSAIDS or other inhibitors or receptor antagonists preclude their use in periodontal therapy.Conclusion: The isolation and characterization of proresolving lipid mediators that are receptor agonists has opened a new area of research for potential therapeutic agents for the management of inflammatory periodontitis.
Micronutritional approaches to periodontal therapy
Authors: Van der Velden U. , Kuzmanova D. , Chapple I. L. C. 
Published: February 2011

Van der Velden U, Kuzmanova D, Chapple ILC. Micronutritional approaches to periodontal therapy. J Clin Periodontol 2011; 38 (Suppl. 11): 142–158. doi: 10.1111/j.1600‐051X.2010.01663.x.AbstractAim: Periodontitis results from the loss of a delicate balance between microbial virulence factors and a proportionate host response. Nutritional factors have been implicated in several chronic inflammatory diseases that are associated with periodontitis. This manuscript reviews the evidence for nutritional exposures in the etiology and therapeutic management of periodontitis, and makes recommendations for daily nutritional intake for vitamin C (ascorbic acid), vitamin D, calcium, and antioxidants.Results and conclusion: Periodontitis is associated with low serum/plasma micronutrient levels, which may result from dietary and/or life‐style factors as well as nutrigenetic characteristics. Early evidence suggests beneficial outcomes from nutritional interventions; supporting the contention that daily intake of certain nutrients should be at the higher end of recommended daily allowances. For prevention and treatment of periodontitis daily nutrition should include sufficient antioxidants, vitamin D, and calcium. Inadequate antioxidant levels may be managed by higher intake of vegetables, berries, and fruits (e.g. kiwi fruit), or by phytonutrient supplementation. Current evidence is insufficient to support recommendations of mono‐antioxidant vitamin supplements and randomised controlled double‐blind intervention studies are needed to provide evidence to underpin future recommendations. Inadequate supply of vitamin D and calcium may be addressed by implementing changes in diet/life style or by supplements.
Do probiotics offer opportunities to manipulate the periodontal oral microbiota?
Authors: Teughels Wim , Loozen Gitte , Quirynen Marc 
Published: February 2011

Teughels W, Loozen G, Quirynen M: Do probiotics offer opportunities to manipulate the periodontal oral microbiota? J Clin Periodontol 2011; 38 (Suppl. 11): 159–177. doi: 10.1111/j.1600‐051X.2010.01665.x. AbstractBackground: As in other fields of healthcare, probiotics have been introduced for prevention and treatment of periodontal diseases.Objective: This review was initiated to explore whether the use of probiotics can influence the periodontal microbiota and periodontal health.Materials and Methods: Literature on the mode of action of oral probiotics was reviewed and a systematic review was performed on the microbiological and clinical effects of oral probiotics on periodontal health.Results: Three animal and 11 in vivo human studies were retrieved. Six studies reported on microbiological effects whereas eight studies report on clinical effects. Seven studies were performed on healthy or gingivitis patients and four studies on periodontitis patients. Many of the retrieved studies are pilot in nature and with low quality. The high degree of heterogeneity between studies hampered analysis.Conclusion: Taking into consideration all limitations, the currently available data indicate an effect of probiotics on the oral microbiota and a more limited effect on clinical periodontal outcome measures. However, there is an urgent need for properly conducted clinical trials where probiotics are used as adjuncts to standard periodontal care, similar to antibiotics, using probiotic strains with, at least at an in vitro level, proven periodontal probiotic effects.
Periodontal microbial complexes associated with specific cell and tissue responses
Authors: Kebschull Moritz , Papapanou Panos N. 
Published: February 2011

Kebschull M, Papapanou PN. Periodontal microbial complexes associated with specific cell and tissue responses. J Clin Periodontol 2011; 38 (Suppl. 11): 17–27. doi: 10.1111/j.1600‐051X.2010.01668.x.AbstractAims: In this review, we summarize data on the association between specific periodontal bacterial profiles and tissue gene/protein expression, generated from cell culture models and in vivo studies.Material and Methods: A PubMed search was conducted to identify publications related to the effects of periodontal microbiota on host cells/tissues.Results and Conclusions: The data indicate the presence of specific host tissue responses to particular microbial complexes, evident by differential regulation of gene or protein expression, ultimately resulting in distinct clinical phenotypes. Transcriptomic analyses showed that periodontal pathogens induce a small, “common core” of differentially regulated genes encoding for an inflammatory response, and a larger variable set of genes that may reflect pathogen‐specific cellular responses. Limitations of available studies include (i) the unclear role of hundreds of subgingival species not yet investigated, (ii) the fact that in vitro studies utilizing single populations of oral cells challenged with mono‐infections of planktonic bacteria may not adequately portray human periodontal diseases and (iii) the cross‐sectional nature of most human studies that makes them inherently incapable of allowing temporal or causal inferences. Longitudinal studies in humans hold the potential to be superior to any model, but need to be adequately powered and controlled.
How is the development of dental biofilms influenced by the host?
Authors: Marsh Philip D. , Devine Deirdre A. 
Published: February 2011

Marsh PD, Devine DA. How is the development of dental biofilms influenced by the host? J Clin Periodontol 2011; 38 (Suppl. 11): 28–35. doi: 10.1111/j.1600‐051X.2010.01673.x.AbstractBackground: The host provides environmental conditions that support diverse communities of microorganisms on all environmentally‐exposed surfaces of the body.Materials and Methods: To review the literature to determine which properties of the host substantially influence the development of dental biofilms.Results: The mouth facilitates the growth of a characteristic resident microbiota. The composition of the oral microbiota is influenced by temperature, pH, and atmosphere, as well as by the host defences and host genetics. In addition, the host supplies endogenous nutrients and a variety of surfaces for biofilm formation. In health, the resident oral microbiota forms a symbiotic relationship with the host, regulated by active host–microbe cross talk. This resident microbiota is sensitive to perturbations in the host environment, especially to changes in nutrient supply and pH, so that previously minor components of the microbiota can become more competitive (and vice versa), resulting in reorganization of biofilm community structure.Conclusion: The host environment dictates the composition and gene expression of the resident microbiota. Changes in oral environmental conditions can disrupt the normal symbiotic relationship between the host and its resident microbes, and increase the risk of disease.
Periodontal infections: understanding the complexity – Consensus of the Seventh European Workshop on Periodontology
Authors: Sanz Mariano , van Winkelhoff Arie Jan ,   on Behalf of Working Group 1 of the Seventh European Workshop on Periodontology
Published: February 2011

AbstractIntroduction: Periodontal diseases are the pathological manifestation of the host response against the bacterial challenge from the dental biofilm at the tooth/gingival interface. The remit of this working group was to update the existing knowledge on the infectious nature of periodontal diseases.Material and Methods: The literature was systematically searched and critically reviewed. Four manuscripts were produced in specific topics identified as key areas to understand the importance of the microorganisms in the etio‐pathogenesis of periodontal diseases.Results/Conclusions: The results and conclusions of the review process are presented in the following papers, together with the group consensus statements aiming to answer the following questions: (1) Has the use of molecular methods for the characterization of the human oral microbiome changed our understanding of the role of bacteria in the pathogenesis of periodontal disease process? (2) Are the periodontal microbial complexes associated with specific cell and tissue responses? (3) How is the development of dental biofilms influenced by the host? (4) What can we learn about biofilm/host interactions from the study of inflammatory bowel disease? This consensus report provides answers to these questions with the most updated information on periodontal microbiology