bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mesenchymal stromal cells based bioengineered implants in vocal folds and tympanic membrane restoration (рilot experimental results)
Authors: Svistushkin M. V. , Bakulina A. A. , Bikmulina P. Yu. , Zolotova A. V. , Selezneva L. V. , Shevchik E. A. , Kochetkov P. A. , Serejnikova N. B. , Nikiforova A. N. , Tychkina I. A. , Shpichka A. I, , Svistushkin V. M. , Timashev P. S. 
Published: May 2024

Introduction. One of the most pressing issues of modern otorhinolaryngology is the problem of the formation of persistent defects of the vocal cords and tympanic membrane. This state of affairs is due to the variety of reasons that cause the development of these conditions, as well as the fact that the treatment of these processes, as a rule, is a technically complex task and is not always effective.Aim. Experimental evaluation of the possibility of using bioengineered implants based on MSCs, PEG-fibrin gel and collagen matrix to restore defects in vibrating tissues of ENT organs – vocal folds and tympanic membrane.Materials and methods. For the experiment on vocal fold restoration, 6 rabbits were selected, divided equally into 2 groups. After the formation of the vocal fold scar, a bioequivalent was implanted: spheroids based on MSCs + PEG-fibrin gel (group – VF-spheroids, n = 3) in a volume of 0.5 ml, for the control group, the role of bioequivalent was performed by saline solution (VF-control group, n = 3). For the eardrum repair experiment, 6 chinchillas were selected, divided equally into 2 groups. At the first stage, a persistent chronic perforation was created, on which either no intervention was carried out (ED-control group, n = 3), or the perforation was closed using a bioequivalent based on MSC spheroids, PEG-fibrin gel and collagen matrix (ED-spheroid group, n = 3).Results and discussion. Endoscopic and morphological examination revealed a more complete restoration of tissue structure in the treatment groups compared to the control groups.Conclusion. Implantation of the developed bioequivalents in the sites of vocal fold and tympanic membrane injury leads to the formation of tissue morphologically similar to the structure of intact structures.
Information systems as a source of meaningful information in an epidemic: experience and lessons in restoring routine otorhinolaryngology care after the COVID-19 pandemic
Authors: Korneenkov A. A. , Ovchinnikov P. A. , Vyazemskaya E. A. , Medvedeva A. Y. , Yanov Yu. K. 
Published: May 2024

Introduction.The COVID-19 pandemic has modified the information needs of participants in the public health management system and identified a lack of information for making management and health decisions during the pandemic.Aim.To summarize and provide a general description of information and data that should be available in the health information system of otorhinolaryngological health facilities for decision-making in epidemic settings based on the results of conducted studies.Materials and methods. In this study we used our own experience in addressing information challenges on optimization of otorhinolaryngological care both at the height of pandemic and after renewal of scheduled visits and hospital admissions. We considered studies under the auspices of WHO on assessment of the effectiveness of health information systems as a whole and individual works that were carried out to analyse the impact of the pandemic on provision of otorhinolaryngological care.Resultsand discussion.The content of data used for decision-making in a pandemic has been substantiated in the following areas: 1) provision of health care should be organized as close as possible to the patient’s personalized care plan with due account for temporal and spatial factors – it is proposed to use patient’s geographic data, patient’s health characteristics indicating the date and time they were registered; 2) health care facilities personnel should be ready to provide medical care – it is proposed to use the results of periodic surveys of health care facilities personnel with regard to their awareness of an epidemic, concern about different risks, working conditions satisfaction, motivation, and emotional status; 3) provision of health care should be simulated, and the optimal option for organizing and distributing the workload should be verified and selected – it is proposed to use service algorithms and necessary resources.
Predictors of morphofunctional features of somatotrophic tumors and of the effectiveness of treatment with first-generation somatostatin receptor ligands
Authors: Pronin E. V. , Pronin V. S. , Antsiferov M. B. , Petryaykin A. V. , Alexeeva T. M. , Lapshina A. M. , Urusova L. S. , Khoruzhaya A. N. , Tamaeva S. M. 
Published: May 2024

Introduction. Timely differential diagnosis between densely and sparsely granulated somatotrophic tumors allows predicting the most probable scenario of acromegaly course, receptor phenotype and proliferative potential of tumor cells, risk of continued postoperative growth, as well as the efficacy of planned drug therapy with first-generation somatostatin receptor ligands.Aim. To validate cytological, radiological and therapeutic predictors allowing to evaluate the morphofunctional composition of somatotrophic tumors and to predict sensitivity to first-generation somatostatin receptor ligands.Materials and methods. 525 patients (153 men) aged 60.2 ± 14.0 years receiving drug therapy with first-generation somatostatin receptor ligands for 72.0 ± 51.9 months were examined.Results. Treatment efficacy was evaluated according to the final level of IGF-1 index (≤1) and compared with the data of pathomorphologic (97 patients) and repeated radiologic (53 patients) examination. The influence of cellular composition of densely and sparsely granulated somatotrophic tumors on immunohistochemical and radiologic characteristics with the designation of leading predictors of morphologic identification of somatotrophic tumors was investigated. Additional radio markers for quantitative assessment of relative intensity of tumor signal on T2-, T1and (T2-T1)-weighted MRI for non-invasive differential diagnostics of densely and sparsely granulated somatotrophic tumors and determination of optimal treatment tactics were proposed. The expediency of using pharmacotherapeutic testing with short-term (3–6 months) first-generation somatostatin receptor ligands administration to assess the intactness of receptor and postreceptor mechanisms and to choose optimal drug therapy was substantiated.Conclusion. The precision approach based on comprehensive clinical, radiological, pathomorphological and functional characteristics allows stratification of patients with acromegaly to optimize treatment benefit. Achievement of acromegaly control in drug therapy with first-generation somatostatin receptor ligands depends on tumor volume and its hormonal activity, absolute and relative expression of the 2nd subtype of somatostatin receptors, severity of destructive changes and features of cellular composition. In case of refractoriness to first-generation somatostatin receptor ligands, the use of pegvisomant is expedient.
Phenylpiracetam: molecular mechanisms of effects in obesity
Authors: Gromova O. A. , Torshin I. Yu. , Lazebnik L. B. 
Published: May 2024

Metabolic syndrome (MS), including hyperlipidemia and obesity, is a proven risk factor not only for cerebrovascular diseases. Obesity is a dangerous comorbid condition in patients, complicating cerebrovascular pathology, asthenic conditions, diabetes mellitus, liver disease, alcoholism and other diseases accompanied by dysmetabolic disorders. Fundamental and clinical studies of the nootropic fonturacetam (Actitropil) have shown that the drug can be used not only for a wide range of cerebrovascular diseases, asthenia, etc., but also for obesity. The mechanisms of action of fonturacetam in producing pharmacological effects that reduce excess appetite and prevent the accumulation of excess body weight were studied in chemoreactomic analysis. Regulation of the metabolic effectiveness of Phenylpiracetam is based on multi-level correction of target transmitters and receptors that control the metabolism of fats and carbohydrates (influence on leptin, cannabinoid receptors, adrenoreceptors, peroxisome receptors). Phenylpiracetam activates the adrenaline, adenosine, glucagon-like peptide, sphingosine phosphate and peroxisome proliferators (PPARG) receptors and inhibits the cannabinoid, opioid, histamine, glutamate, nociceptin, orexin, neuropeptide Y receptors. The resulting pharmacological properties indicate important pathophysiological effects of phenylpiracetam for the treatment of obesity. A decrease in the rate of fat mass gain when taking Phenylpiracetam is noted due to an improvement in the quality of night sleep. Chemoreactomic analysis of Actitropil indicated new molecular mechanisms of the pharmacological action of the molecule, which reduces excess appetite and prevents the accumulation of excess body weight. Phenylpiracetam (Actitropil) is distinguished by a balance of effectiveness, a high safety profile with no addiction to the drug and safety. Thus, Phenylpiracetam is a racetam that exhibits nootropic, antiasthenic and lipotropic effects.
Recent Advances in Trifluoromethylation of Olefins, Aldehydes, and Ketones
Authors: Bai Mengran , Zhang Liyuan , Liu Lu , Jia Chenyu , Zheng Yuting , Shang Huijian , Sun Hui , Cui Bin 
Published: May 2024

:
Due to the robust electrophilic properties of the trifluoromethyl group (-CF3), its
incorporation into organic compounds can markedly alter their ester affinity, stability, bioavailability,
and other properties. The trifluoromethylation reaction is currently experiencing
rapid advancement, with an expanding array of substrates and the emergence of novel
methodologies. Consequently, compounds containing the -CF3 moiety find extensive utility
across diverse fields. This article aims to comprehensively review the latest advancements
in trifluoromethylation reaction of olefins, aldehydes, and ketones, encompassing
nucleophilic trifluoromethylation, electrophilic trifluoromethylation, and radical trifluoromethylation.
The discussion includes an exploration of the types and broadening scope of
applicable substrates. Furthermore, this article addresses the associated challenges and delineates
prospective directions for future developments in trifluoromethyl reaction.
The Effectiveness of LISTEXTM P100 in Reducing the Biofilm of Listeria spp. on Steel, Plastic, and Galvanised Surfaces
Authors: AlMatar Manaf , Var Isil , Saglam Selin , Albarri Osman 
Published: May 2024

Background:
Eliminating and managing L. monocytogenes, L. welshimeri, and L.
ivanovii biofilms is a significant problem for food safety, as listeriosis is among the worst foodborne
illnesses.

Methods:
The Listex P100 bacteriophage's bactericidal and inhibitory properties have been investigated
in relation to varying strains of vegetative cells and biofilms of L. monocytogenes, L.
welshimeri, and L. ivanovii.

Results:
The phage concentrations of 109 and 1010 PFU/ml showed strong antibacterial activity
against L. monocytogenes, L. welshimeri, and L. ivanovii at both 10°C and 30°C (P<0.05). In 96-
well microplate experiments, bacteriophage treatment inhibited biofilm development and reduced
biofilm by up to 57.6% (P ≤ 0.05). When compared to controls, Listex P100 bacteriophage
significantly reduced the populations of L. monocytogenes, L. welshimeri, and L. ivanovii
biofilms on the surfaces of galvanised, stainless steel, and plastic surfaces where holes were
produced and the structure of Listeria spp. was disturbed.

Conclusion:
This study clearly demonstrated that L. monocytogenes, L. welshimeri, and L. ivanovii
biofilms on galvanised, stainless steel, and plastic surfaces might be removed by using
Listex P100 bacteriophage.
Oleoresin Capsicum (OC) Spray: An Assessment of Respiratory Health
and its Management Following Accidental and Deliberate Exposures
Authors: Patowary Pompy , Pathak Manash Pratim , Barbhuiya Pervej Alom , Karmakar Sanjeev , Chattopadhyay Pronobesh , Zaman Kamaruz 
Published: May 2024

Abstract:
Introduced into law enforcement in 1976, the oleoresin capsicum (OC) spray has been
labeled as one of the most significant and radical developments in law enforcement. However, epidemiological
research on OC health effects is deficient, receiving little public support. The major
responses to acute exposure to OC spray can be found in the pulmonary system. The molecular
mechanism(s) involved in the action of capsaicinoids, the active constituents in OC, are complex
cascades of reactions which end up in necrosis or apoptosis. OC may also damage and deplete biological
redox systems in the epithelial lining fluids and within cells and mitochondria, modifying
structural proteins and nucleic acids and leading to enzyme inactivation. Since there are no characteristic
laboratory tests available for identification or confirmation of OC exposure, and on the basis
of prevailing data, reassessment of the health risks of OC exposures in vulnerable populations
and in-depth study of the molecular mechanics of receptors is the need of the hour for the development
of effective countermeasures. This review aims to consider evidence for adverse effects of
OC spray used in ways comparable to their application by law enforcement personnel and
civilians, with possible treatment recommendations that are precedent for improved management.
Construction of an Adenovirus Vector with Laco-regulated Antigen Expression Using the SARS-Cov-2 Spike as a Transgene Model
Authors: Artarini Anita , Bassalamah Annisa Rahma , Arbuati Arrie Arifa , Hadianti Tia , Prasetya Katherine Vanya , Tan Marselina Irasonia , Natalia Dessy , Riani Catur , Giri-Rachman Ernawati Arifin 
Published: May 2024

Background:
The adenovirus vector has been widely studied for vaccines and gene therapies.
During the production of the adenovirus vector, a high virus titer is desired to obtain enough
virus. The adenovirus vector has been widely studied for the vaccinations and gene therapies, where
a high virus titer is desired to obtain sufficient quantities of the virus. For an adenovirus vector-based
vaccine, suppression of antigen expression during production would improve the virus titer during
production.

Objective:
This study aimed to construct an adenovirus vector with lacO-regulated antigen expression
using the SARS-CoV-2 spike as a transgene model, which would improve the adenovirus titer
during production.

Methods:
The lacO expression cassette was designed and prepared as a synthetic gene in pUC57.
The lacO expression cassette was then subcloned into pShuttle-CMV. The SARS-CoV-2 spike gene
was then inserted into the pShuttle-CMV harboring lacO to generate pShuttle-lacO_S and pShuttlelacO-
intron_S. Recombinant pShuttle was then used to generate a recombinant adenovirus genome
using Escherichia coli BJ5183 pAdeasy-1. Transfection of the PacI-linearized adenovirus genome into
AD293 and HEK293 cells was used to generate adenovirus primary stock for 14 days of incubation.

Results:
Recombinant adenovirus genomes, pAdeasy-lacO_S and pAdeasy-lacO-intron_S, were
successfully generated and characterized using PacI restriction and PCR. In the production of adenovirus
primary stocks, the adenovirus titer produced in AD293 cells was higher than in HEK293
cells. The primary stock titer of AdV_lacO-intron-S was higher than AdV_lacO-S and AdV_S titers.

Conclusion:
Production of adenovirus with lacO and spike gene, either with or without intron, was
successful with a higher titer as compared to AdV_S titer.
Experience of Implementation of the Lean Production Concept at the Enterprise of Thermal Insulating Materials Market
Authors: Kondakov M. V. 
Published: May 2024

Relevance. The construction industry produces a third of global waste, much of it is not recycled, ending up in landfills and polluting the environment. Between 2000 and 2020, the volume of construction work has increased more than 12-fold. The flip side of the process is a proportional increase in waste. In the insulation materials market and in the construction industry in general, the relevance of the lean manufacturing concept is growing, as it can be used to reduce the amount of resources consumed and increase business efficiency.Purpose. This article considers the developed measures for implementation of the concept of lean production in the framework of strategic management of the enterprise at the plant of the market of thermal insulation materials.Objectives. Within the framework of the concept realization the excessive quality is reduced, the system of personnel training is implemented and the level of competitiveness of the enterprise is increased.Methodology. The object of the study is the plant of Belgorod region, the leader in labor productivity in the city of Belgorod. The works were carried out from 2021 to 2023.Results. Due to the implemented program labor productivity increased by 2,0%, the number of scrap was reduced from 1,7% to 0,8%, new strategies for the development of the company were developed due to the introduction of personnel training system.Conclusions. The program of increase of competitiveness of the enterprise of branch of heat-insulating materials on the basis of introduction of the concept of lean production at its projection on other companies given in the present article is perspective in the plan of increase of competitiveness of the Russian Federation in the world arena, in fact the economy of the country depends on efficiency of subjects functioning in it.
An Integrative Approach to the Development of Inclusive Tourism: a Model of Multilevel Interaction of Service Market Entities
Authors: Mustafina A. V. , Pirogova O. E. 
Published: May 2024

Relevance. Today, along with the development of inclusive tourism, there is a need for balanced interaction between its participants. This interaction is an essential element for achieving sustainable development of inclusive tourism in the long term.The purpose is development of a model of interaction in the field of inclusive tourism aimed at optimizing cooperation between key participants in this industry.Objectives: to determine the role of the state in the development of inclusive tourism, to form a model of the external environment of interaction in inclusive tourism, to analyze the interaction between government agencies, the private sector, non-governmental organizations and consumers in the context of inclusive tourism.Methodology. The research methodology included the use of subjective, systemic, process and functionalenvironmental approaches to analyze the internal and external environment of interaction in inclusive tourism.Results. Currently, the problem of inclusive tourism is becoming urgent, which is the newest method of rehabilitation and restoration of human well-being. The intersectoral tourism complex acts as a catalyst for integration and innovation, and supports a variety of tourism products and services. The UNWTO model is universal, but unsuitable for a number of criteria for the specific conditions of individual regions. The State forms a framework for the development of inclusive tourism and participates in the creation of departments and committees that interact with various sectors of the economy and tourism organizations. The presented comprehensive model of interaction is based on the principles of synergy and information exchange between stakeholders.Conclusions. Effective communication between participants in the tourism market can be achieved through the development and provision of adapted technologies. It is necessary to create an accessible infrastructure, protect the rights and interests of inclusive tourists through joint efforts and the exchange of knowledge and resources. An inclusive tourism system should include not only the adaptation of infrastructure and services, but also educational initiatives. There should be an integrated approach that ensures a strong link between the State, the private sector and public organizations.
Marketplace: Business Model of the Modern Economy
Authors: Pyanova N. V. , Salenkova S. A. , Pyanov R. R. , Kryzhanovskaya O. A. 
Published: May 2024

Relevance. The study of the features of the work and development of marketplaces is a relevant area, since these platforms play a significant role in modern society and business, representing a business model of the modern economy. They bring together sellers and buyers, providing the opportunity to sell goods and services over large territories and increase their customer base. Additionally, analyzing the key features of popular e-commerce platforms can help entrepreneurs choose the most suitable platform for their business and determine its advantages and disadvantages. Online stores offer a wide range of products, convenient delivery and discounts for regular customers. In addition, many marketplaces work directly with manufacturers, which allows them to offer products at lower prices. Thus, we can assert the relevance of the study of marketplaces as a business model of the modern economy.The purpose of the study is to study the features of the operation of distance trading platforms - marketplaces, the history of their origin, the impact on purchasing behavior, key characteristics, as well as the advantages and disadvantages of popular e-commerce platforms.Objectives: identifying the weaknesses of online stores; identifying possible ways to solve problems arising in this area of business.Methodology. The following methods were used to study online stores: data analysis, user survey, comparison of various platforms, study of reviews and ratings.Results. Selling through marketplaces is the business model of the modern economy. It is necessary to take into account the peculiarities of the functioning of marketplaces, their differences from other forms of sales of goods in the conditions of applying a modern business model that is adequate to the real conditions of doing business and economic development.Conclusions. The use of marketplaces is associated with the ability to minimize the costs of production, sales, purchase or exchange based on building effective interaction on a digital platform and various adaptive combinations of business processes. This allows us to prove the initial assumption that the marketplace is a business model of the modern economy.