bluuebunny/crossref_metadata_2025_split huggingface dataset:
Alteration in neural oscillatory activity and phase‐amplitude coupling after sleep deprivation: Evidence for impairment and compensation effects
Authors: Liu Zehui , Zhou Yuqi , Hao Chao , Ma Ning 
Published: June 2024

SummaryInsufficient sleep can significantly affect vigilance and increase slow‐wave electroencephalographic power as homeostatic sleep pressure accumulates. Phase‐amplitude coupling is involved in regulating the spatiotemporal integration of physiological processes. This study aimed to examine the functional associations of resting‐state electroencephalographic power and delta/theta‐gamma phase‐amplitude coupling from the prefrontal cortex (PFC) to posterior regions with vigilance performance after sleep deprivation. Forty‐six healthy adults underwent 24‐hr sleep deprivation with resting‐state electroencephalographic recordings, and vigilant attention was measured using the Psychomotor Vigilance Task. Power spectral and phase‐amplitude coupling analyses were conducted, and correlation analysis was utilized to reveal the relationship between electroencephalographic patterns and changes in vigilance resulting from sleep deprivation. Sleep deprivation significantly declined vigilance performance, accompanied by increased resting‐state electroencephalographic power in all bands and delta/theta‐gamma phase‐amplitude coupling. The increased theta activity in centro‐parieto‐occipital areas significantly correlated with decreased mean and slowest response speed. Conversely, the increased delta‐low gamma and theta‐high gamma phase‐amplitude couplings negatively correlated with the deceleration of the fastest Psychomotor Vigilance Task reaction times. These findings suggest that sleep deprivation affects vigilance by altering electroencephalographic spectral power and information communication across frequency bands in different brain regions. The distinct effects of increased theta power and delta/theta‐gamma phase‐amplitude coupling might reflect the impairment and compensation of sleep deprivation on vigilance performance, respectively.
Earnings assimilation of post‐reunification East German migrants in West Germany
Authors: Riphahn Regina T. , Sauer Irakli 
Published: June 2024

AbstractWe investigate the wage assimilation of East Germans who migrated to West Germany after reunification (1990–99). We compare their wage assimilation to that of ethnic German immigrants from Eastern Bloc countries and international immigrants to West Germany who arrived at the same time. The analysis uses administrative as well as survey data. The results suggest that East Germans faced significant initial earnings disadvantages in West Germany, even conditional on age and education. However, these disadvantages were smaller than those of international immigrants, supporting the beneficial role of cultural similarity. The earnings gap relative to West German natives narrowed over time for all immigrants. These findings are robust to controlling for potentially endogenous return migration and labor force participation. Controls for fixed effects reveal that positive assimilation for East German and international immigrants was concentrated among highly educated immigrants.
From whole bodies to single cells: A guide to transcriptomic approaches for ecology and evolutionary biology
Authors: Hoedjes Katja M. , Grath Sonja , Posnien Nico , Ritchie Michael G. , Schlötterer Christian , Abbott Jessica K. , Almudi Isabel , Coronado‐Zamora Marta , Durmaz Mitchell Esra , Flatt Thomas , Fricke Claudia , Glaser‐Schmitt Amanda , González Josefa , Holman Luke , Kankare Maaria , Lenhart Benedict , Orengo Dorcas J. , Snook Rhonda R. , Yılmaz Vera M. , Yusuf Leeban 
Published: June 2024

AbstractRNA sequencing (RNAseq) methodology has experienced a burst of technological developments in the last decade, which has opened up opportunities for studying the mechanisms of adaptation to environmental factors at both the organismal and cellular level. Selecting the most suitable experimental approach for specific research questions and model systems can, however, be a challenge and researchers in ecology and evolution are commonly faced with the choice of whether to study gene expression variation in whole bodies, specific tissues, and/or single cells. A wide range of sometimes polarised opinions exists over which approach is best. Here, we highlight the advantages and disadvantages of each of these approaches to provide a guide to help researchers make informed decisions and maximise the power of their study. Using illustrative examples of various ecological and evolutionary research questions, we guide the readers through the different RNAseq approaches and help them identify the most suitable design for their own projects.
The proteome of Nicotiana benthamiana is shaped by extensive protein processing
Authors: Zheng Kaijie , Lyu Joy C. , Thomas Emma L. , Schuster Mariana , Sanguankiattichai Nattapong , Ninck Sabrina , Kaschani Farnusch , Kaiser Markus , van der Hoorn Renier A. L. 
Published: June 2024

Summary

Processing by proteases irreversibly regulates the fate of plant proteins and hampers the production of recombinant proteins in plants, yet only few processing events have been described in agroinfiltrated Nicotiana benthamiana, which has emerged as the main transient protein expression platform in plant science and molecular pharming.

Here, we used in‐gel digests and mass spectrometry to monitor the migration and topography of 5040 plant proteins within a protein gel. By plotting the peptides over the gel slices, we generated peptographs that reveal where which part of each protein was detected within the protein gel.

These data uncovered that 60% of the detected proteins have proteoforms that migrate at lower than predicted molecular weights, implicating extensive proteolytic processing. This analysis confirms the proteolytic removal and degradation of autoinhibitory prodomains of most but not all proteases, and revealed differential processing within pectinemethylesterase and lipase families. This analysis also uncovered intricate processing of glycosidases and uncovered that ectodomain shedding might be common for a diverse range of receptor‐like kinases. Transient expression of double‐tagged candidate proteins confirmed processing events in vivo.

This large proteomic dataset implicates an elaborate proteolytic machinery shaping the proteome of N. benthamiana.
Public Employees' whistleblowing intention: Explanation by an adapted theory of planned behavior
Authors: Zhang Yahong , Hung Wan‐Ju , Dong Hsiang‐Kai , Chen Don‐Yun 
Published: June 2024

AbstractPublic employees' whistleblowing is an effective anti‐corruption means because they can provide rigorous evidence of wrongdoing in public organizations. However, whistleblowing may cause risks to the individuals and their organization, which discourage them from reporting. While the topic has been understudied, a few studies have adopted the theory of planned behavior (TPB) from psychology to gain insights into whistleblowing. However, these studies have overlooked the unique characteristics of the public sector and the contextual factors surrounding whistleblowing. Our research aims to fill this gap by incorporating these missing elements into the theoretical framework and thus developing an adapted TPB. Using survey data from Taiwan, this study provides empirical evidence to support the adapted TPB in explaining the intention of public employees to blow the whistle.Chinese Abstract由公務人員本身來舉報不法事件, 被視為是一種相當有效的反貪腐手段, 因為他們通常能夠提供這些不當行為的有力證據。然而, 舉報不法事件卻可能會對公務人員個人或其單位帶來風險, 也因此往往成為其最終選擇沉默的主要原因。雖然過去已有一些研究採取「計劃行為理論 (TPB)」來探討此一主題, 但這些研究卻沒有考量公部門獨有的特性及環境系絡對舉報行為的影響。本研究希望能夠整合這些過去被遺漏的要素, 並且發展出更適合公部門的理論框架。本研究使用臺灣文官調查 (TGBS) 所蒐集的資料, 進行實證分析並用更完整的 TPB 模型來解釋公職人員舉報不法的行為意圖。.
Journey through the spectacular landscape of melanocortin 1 receptor
Authors: Upadhyay P. R. , Swope V. B. , Starner R. J. , Koikov L. , Abdel‐Malek Z. A. 
Published: June 2024

AbstractThe physiological role of α‐melanocyte stimulating hormone in regulating integumental pigmentation of many vertebrate species has been recognized since the 1960's. However, its physiological significance for human pigmentation remained enigmatic until the 1990's. α‐Melanocyte stimulating hormone and related melanocortins are synthesized locally in the skin, primarily by keratinocytes, in addition to the pituitary gland, and therefore act as paracrine factors for melanocytes. Human melanocytes express the melanocortin 1 receptor, which recognizes α‐melanocyte stimulating hormone and the related adrenocorticotropic hormone as agonists. This review summarizes the current knowledge of the pleotropic effects of the activated melanocortin 1 receptor that maintain human melanocyte homeostasis by regulating melanogenesis and the response to environmental stressors, mainly solar radiation. Certain allelic variants of the melanocortin 1 receptor gene are associated with specific pigmentary phenotypes in various human populations. Variants associated with red hair phenotype compromise the function of the encoded receptor. Activation of the human melanocortin 1 receptor regulates eumelanin synthesis and enhances DNA damage response of melanocytes to solar radiation and oxidative stressors. We describe how synthetic selective melanocortin 1 receptor agonists can be efficacious as sunless tanning agents, for treatment of vitiligo and photosensitivity disorders, and for prevention of skin cancer, including melanoma.
‘It's all there in the language’—a conversation with Garrett Stewart
Authors: LaRocca David 
Published: June 2024

AbstractWhat does a famed literary theorist have to say about the interaction between ‘literature’ and ‘philosophy’? Well, if he's Garrett Stewart, the celebrated agent of pyrotechnic style in the service of durable insights across disparate disciplines and media, then we have much reason to lean in and listen. Stewart is the author of 20 books that range with uncanny competency across Victorian narrative, contemporary American fiction, written auralities, poetics and prose stylistics, cinematic evolution from silver oxide to screen pixel, book art, scenes of reading in painting, and most recently, the vocal drama of Barbra Streisand. The distances between his disciplinary terrains are as vast as his close readings of them are coordinated and trenchant. Here, sitting down before a shared conversational canvas, Stewart fields questions from David LaRocca, who asks how he, if he, thinks about the program of literature and philosophy. LaRocca, editor of the recent Attention Spans: Garrett Stewart, a Reader (Bloomsbury, 2024) and the forthcoming Bandwidths: Reading Across Media with Garrett Stewart (Bloomsbury), tracks the urgency of our moment—in which literature and philosophy are independently besieged and yet may concede little rapprochement. Where does philosophy happen in literature (a philosopher's question) is turned around by Stewart such that we see literature conjuring philosophy at the level of the sentence. We are not peering ‘in’ so much as looking ‘at’ the art of literature teaching philosophy through refined reading and the analytical inquest of the syllable and the sayable. Anyone who works at the fraught fissure of these realms will benefit from the ways Stewart makes literature and philosophy sensible to one another—even as he productively unsettles the terms and conditions of their affiliation.
The effect of motivational interviewing on quality of life and self‐efficacy behaviors of individuals with chronic illness: A meta‐analysis study
Authors: Uzun Sevda , Gürhan Nermin 
Published: June 2024

AbstractObjectiveThis study aims to reveal the effect of the motivational interviewing on the quality of life and self‐efficacy behaviors of individuals with chronic diseases.Material‐methodFor this meta‐analysis study, PubMed, Web of Science, EBSCOhost, Google Scholar, and YÖK Theses databases were searched in January–May 2021 without year limitation. As a result, 38 studies were included in the study: 23 for the quality of life variable, and 21 for the self‐efficacy behaviors variable (in 6 studies, both variables were examined together). The data were synthesized with meta‐analysis and narrative methods. The total sample size of the studies is 25,425.ResultsThis meta‐analysis study showed that the motivational interviewing applied to individuals with chronic diseases changed their quality of life (SMD: 0.296, %95 CI: 0.054–0.537, Z = 2.402, p = .016, I2 = %95). The type of chronic disease, measurement tool, sample group, disease group, age range, and whether the motivational interviewer was a nurse or not changed the effect size of the motivational interviewing on the quality of life. The self‐efficacy score average of the experimental group to which motivational interviewing was applied was statistically similar to that of the control group (SMD: 0.141, %95 CI: −0.065, 0.347; Z = 1.346, p = .178, I2 = %87).ConclusionsAs a result of the meta‐analysis, it was determined that the use of motivational interviewing increased the quality of life level of individuals with chronic diseases. However, it is thought that more research is needed because some studies were conducted with small sample size and prepost test design. The results of the study may provide guidance on the interventions to be used in improving the quality of life of individuals with chronic diseases.
Enhancing drought tolerance in chilli pepper through AdDjSKI‐mediated modulation of ABA sensitivity, photosynthetic preservation, and ROS scavenging
Authors: Bulle Mallesham , Venkatapuram Ajay Kumar , Rahman Md. Mezanur , Attia Kotab A. , Mohammed Arif Ahmed , Abbagani Sadanandam , Kirti P. B. 
Published: June 2024

AbstractDrought stress threatens the productivity of numerous crops, including chilli pepper (Capsicum annuum). DnaJ proteins are known to play a protective role against a wide range of abiotic stresses. This study investigates the regulatory mechanism of the chloroplast‐targeted chaperone protein AdDjSKI, derived from wild peanut (Arachis diogoi), in enhancing drought tolerance in chilli peppers. Overexpressing AdDjSKI in chilli plants increased chlorophyll content, reflected in the maximal photochemical efficiency of photosystem II (PSII) (Fv/Fm) compared with untransformed control (UC) plants. This enhancement coincided with the upregulated expression of PSII‐related genes. Our subsequent investigations revealed that transgenic chilli pepper plants expressing AdDjSKI showed reduced accumulation of superoxide and hydrogen peroxide and, consequently, lower malondialdehyde levels and decreased relative electrolyte leakage percentage compared with UC plants. The mitigation of ROS‐mediated oxidative damage was facilitated by heightened activities of antioxidant enzymes, including superoxide dismutase, catalase, ascorbate peroxidase, and peroxidase, coinciding with the upregulation of the expression of associated antioxidant genes. Additionally, our observations revealed that the ectopic expression of the AdDjSKI protein in chilli pepper plants resulted in diminished ABA sensitivity, consequently promoting seed germination in comparison with UC plants under different concentrations of ABA. All of these collectively contributed to enhancing drought tolerance in transgenic chilli plants with improved root systems when compared with UC plants. Overall, our study highlights AdDjSKI as a promising biotechnological solution for enhancing drought tolerance in chilli peppers, addressing the growing global demand for this economically valuable crop.
Neighborhood‐level racialized socioeconomic deprivation and contraceptive use in the United States, 2011–2019
Authors: Cartwright Alice F. , Wallace Maeve , Su Jessica , Curtis Siân , Angeles Gustavo , Speizer Ilene S. 
Published: June 2024

AbstractIntroductionThe social and structural environments where people live are understudied in contraceptive research. We assessed if neighborhood measures of racialized socioeconomic deprivation are associated with contraceptive use in the United States.MethodsWe used restricted geographic data from four waves of the National Survey of Family Growth (2011–2019) limited to non‐pregnant women ages 15–44 who had sex in the last 12 months. We characterized respondent neighborhoods (census tracts) with the Index of Concentration at the Extremes (ICE), a measure of spatial social polarization, into areas of concentrated privilege (predominantly white residents living on high incomes) and deprivation (predominantly people of color living on low incomes). We used multivariable binary and multinomial logistic regression with year fixed effects to estimate adjusted associations between ICE tertile and contraceptive use and method type. We also assessed for an interactive effect of ICE and health insurance type.ResultsOf the 14,396 respondents, 88.4% in neighborhoods of concentrated deprivation used any contraception, compared to 92.7% in the most privileged neighborhoods. In adjusted models, the predicted probability of using any contraception in neighborhoods of concentrated deprivation was 2.8 percentage points lower than in neighborhoods of concentrated privilege, 5.0 percentage points higher for barrier/coital dependent methods, and 4.3 percentage points lower for short‐acting methods. Those with Medicaid were less likely to use any contraception than those with private insurance irrespective of neighborhood classification.ConclusionsThis study highlights the salience of structural factors for contraceptive use and the need for continued examination of structural oppressions to inform health policy.
Origin of the H1N1 (Russian influenza) pandemic of 1977—A risk assessment using the modified Grunow–Finke tool (mGFT)
Authors: Kalyar Fatema , Chen Xin , Chughtai Abrar Ahmad , MacIntyre Chandini Raina 
Published: June 2024

AbstractIn 1977, the Soviet Union (Union of Soviet Socialist Republics [USSR]) notified the World Health Organization (WHO) about an outbreak of H1N1 influenza, which later spread to many countries. The H1N1 strain of 1977 reappeared after being absent from the world for over 20 years. This pandemic simultaneously spread to several cities in the USSR and China. Many theories have been postulated to account for the emergence of this pandemic, including natural and unnatural origins. The purpose of this study was to use the modified Grunow–Finke risk assessment tool (modified Grunow‐Finke tool [mGFT]) to investigate the origin of the 1977 H1N1 pandemic. Data was collected from WHO archives and published documents. The assessment of the pandemic's origin involved the utilization of a modified version of the original Grunow–Finke risk assessment tool (GFT). Using the mGFT, the final score was 37 out of 60 points (probability: 62%), indicating a high likelihood that the Russian influenza pandemic of 1977 was of unnatural origin. Several variables supported this finding, including the sudden re‐emergence of a previously extinct strain, a genetic signature of laboratory modification for vaccine development, and unusual epidemiology. Inter‐rater reliability was moderate to high. By applying the mGFT to the 1977 Russian influenza pandemic, we established a high probability that this pandemic was of unnatural origin. Although this is not definitive, it is consistent with the possibility that it originated from an incompletely attenuated live influenza vaccine. The mGFT is a useful risk analysis tool to evaluate the origin of epidemics.
Through Thick and Thin: Gesture and Speech Remain as an Integrated System in Atypical Development
Authors: Demir‐Lira Ö. Ece , Göksun Tilbe 
Published: June 2024

AbstractGesture and speech are tightly linked and form a single system in typical development. In this review, we ask whether and how the role of gesture and relations between speech and gesture vary in atypical development by focusing on two groups of children: those with peri‐ or prenatal unilateral brain injury (children with BI) and preterm born (PT) children. We describe the gestures of children with BI and PT children and the relations between gesture and speech, as well as highlight various cognitive and motor antecedents of the speech‐gesture link observed in these populations. We then examine possible factors contributing to the variability in gesture production of these atypically developing children. Last, we discuss the potential role of seeing others’ gestures, particularly those of parents, in mediating the predictive relationships between early gestures and upcoming changes in speech. We end the review by charting new areas for future research that will help us better understand the robust roles of gestures for typical and atypically‐developing child populations.
Money matters: The association between blood donation rates and healthcare system quality across 171 countries
Authors: Hoorn Lieke C. , Graf Caroline , Merz Eva‐Maria 
Published: June 2024

AbstractBackgroundWorldwide, insufficient blood donations cause blood shortages that jeopardize vital medical treatments for patients. Blood donation rates vary widely across countries, yet the determinants of this variation remain poorly understood. This study aims to illuminate the role of the institutional context in which blood donation is embedded by examining the link between country‐level blood donation rates and healthcare system quality.Study Design and MethodsThe study employed a cross‐sectional design using data on blood donation rates from 171 countries from the 2021 WHO Global Status Report on Blood Safety and Availability and three healthcare quality indicators (i.e., Healthcare Access and Quality [HAQ] Index, life expectancy, and health expenditures). The pre‐registered hypotheses are tested using multiple linear regression. Robustness checks control for confounding factors.ResultsHAQ Index and health expenditures are positively associated with blood donation rates, whereas life expectancy is not related to blood donation when controlling for confounds. Health expenditures display the most robust association with blood donations, even when controlling for confounding factors, and when comparing countries within the same continent.ConclusionHigher healthcare system quality in terms of HAQ Index and higher healthcare expenditures are related to higher blood donation rates. The finding that healthcare expenditures are most consistently related to blood donation rates indicates that policymakers should consider prioritizing financial support for the healthcare system, including blood‐collecting institutions. More broadly, a better understanding of the role of contextual factors for blood donation may be needed to increase blood availability worldwide.
Intradural‐extramedullary inflammatory pseudotumour of the cervical spinal cord of a dog
Authors: Reyes Henry Mendo , Fiorentino Erica , Matiasek Kaspar , Menchetti Marika 
Published: June 2024

AbstractAn 8‐year‐old mixed‐breed dog was presented with cervical hyperesthesia, tetraparesis, and mild proprioceptive ataxia in all four limbs. 3 Tesla MRI showed a dorsal compressive intradural‐extramedullary mass at the level of C1–C2, isointense to the gray matter with a hypointense ventral core on T2 weighted images (WI), isointense on T1WI, with a strong and homogeneous contrast enhancement. A C1–C2 partial dorsal laminectomy was performed, and the lesion was removed en bloc. The histopathological and immunohistochemical analysis defined the diagnosis of inflammatory pseudotumor.