bluuebunny/crossref_metadata_2025_split huggingface dataset:
Gene Expression Profile of BRCAness That Correlates With Responsiveness to Chemotherapy and With Outcome in Patients With Epithelial Ovarian Cancer
Authors: Konstantinopoulos Panagiotis A. , Spentzos Dimitrios , Karlan Beth Y. , Taniguchi Toshiyasu , Fountzilas Elena , Francoeur Nancy , Levine Douglas A. , Cannistra Stephen A. 
Published: June 2010

Purpose To define a gene expression profile of BRCAness that correlates with chemotherapy response and outcome in epithelial ovarian cancer (EOC). Methods A publicly available microarray data set including 61 patients with EOC with either sporadic disease or BRCA½ germline mutations was used for development of the BRCAness profile. Correlation with platinum responsiveness was assessed in platinum-sensitive and platinum-resistant tumor biopsy specimens from six patients with BRCA germline mutations. Association with poly-ADP ribose polymerase (PARP) inhibitor responsiveness and with radiation-induced RAD51 foci formation (a surrogate of homologous recombination) was assessed in Capan-1 cell line clones. The BRCAness profile was validated in 70 patients enriched for sporadic disease to assess its association with outcome. Results The BRCAness profile accurately predicted platinum responsiveness and mutation status in eight of 10 patient-derived tumor specimens and between PARP-inhibitor sensitivity and resistance in four of four Capan-1 clones. When applied to the 70 patients with sporadic disease, patients with the BRCA-like (BL) profile had improved disease-free survival (34 months v 15 months; log-rank P = .013) and overall survival (72 months v 41 months; log-rank P = .006) compared with patients with a non–BRCA-like (NBL) profile, respectively. The BRCAness profile maintained independent prognostic value in multivariate analysis, which controlled for other known clinical prognostic factors. Conclusion The BRCAness profile correlates with responsiveness to platinum and PARP inhibitors and identifies a subset of sporadic patients with improved outcome. Additional evaluation of this profile as a predictive tool in patients with sporadic EOC is warranted.
Procoagulant Activity in Trauma Patients
Authors: Chandler Wayne L. 
Published: June 2010

Abstract
Elevated levels of circulating procoagulants like tissue factor may increase the risk of systemic coagulation activation, thrombin generation, and consumptive coagulopathy. I measured procoagulant activity in plasma by using a clot-based assay that incorporated normal plasma to replace missing factors, corn trypsin inhibitor to block contact activation, factor VIIa to improve sensitivity to tissue factor activity, and anti–tissue factor antibodies to measure tissue factor–specific activity. Procoagulant activity was evaluated in 58 trauma patients. Trauma patients without coagulopathy (n = 50) showed 5-fold higher procoagulant activity than did control subjects (P &lt; .001), whereas trauma patients with coagulopathy (n = 8) showed 10-fold higher activity than control subjects (P &lt; .001) and 2-fold higher activity than trauma patients without coagulopathy (P = .03). In control subjects, tissue factor activity was below the detection limit of the assay. Tissue factor activity was 3- to 4-fold higher in trauma patients with coagulopathy vs patients without coagulopathy (P = .002). Trauma patients with coagulopathy have increased circulating tissue factor activity.
A Novel Thromboelastographic Score to Identify Overt Disseminated Intravascular Coagulation Resulting in a Hypocoagulable State
Authors: Sharma Prashant , Saxena Renu 
Published: June 2010

Abstract
Thromboelastography (TEM) yields a multitude of data that are complicated to analyze. We evaluated its value in identification of global coagulopathy in overt disseminated intravascular coagulation (DIC). We studied 21 patients, each with International Society for Haemostasis and Thrombosis scores of 5 or more (compatible with overt DIC) and less than 5 (suggestive of nonovert DIC), who underwent whole blood nonadditive TEM. A TEM score based on the reaction and κ times, α angle, and maximum amplitude was defined as the total number of TEM parameters deranged in the direction of hypocoagulability.
The TEM score at a cutoff of 2 or more achieved sensitivity of 95.2%, specificity of 81.0%, and the highest receiver operating characteristic area under the curve of all parameters of 0.957 for identifying overt DIC. Individual TEM parameters correlated variably with conventional tests. Their combination into a cohesive TEM score possibly better captured the multiple hemostatic derangements occurring in DIC. The TEM score may bring objectivity to the analysis of TEM data.
Significance of PGP9.5 Expression in Cancer-Associated Fibroblasts for Prognosis of Colorectal Carcinoma
Authors: Akishima-Fukasawa Yuri , Ino Yoshinori , Nakanishi Yukihiro , Miura Ayaka , Moriya Yoshihiro , Kondo Tadashi , Kanai Yae , Hirohashi Setsuo 
Published: June 2010

Abstract
To assess the expression of a cancer-associated fibroblasts (CAFs) marker as an indicator of prognosis, we raised anti–protein gene product 9.5 (PGP9.5) monoclonal antibody against cultured fibroblasts. PGP9.5 expression in cultured normal fibroblasts was increased by transforming growth factor β stimulation, indicating the phenotypic alteration to activated fibroblast. We immunohistochemically evaluated PGP9.5 expression with the CAFs of 110 colorectal cancer cases under T3 stage. PGP9.5 immunoreactivity in 30% or more of CAFs was defined as high PGP9.5 expression, and the other cases were considered as having low PGP9.5 expression.
Patients with high PGP9.5 expression (42.7%) had significantly shorter survival and a higher incidence of recurrence than the low PGP9.5 expression group (P = .002 and P &lt; .001, respectively). Multivariate analysis indicated PGP9.5 expression as an independent prognostic factor for overall and recurrence-free survival partly as well as lymph node metastasis. These results indicate that PGP9.5 expression in CAFs is a helpful finding to represent the overall biologic behavior of advanced colorectal cancer.
Impact of Caregiver Literacy on Children's Oral Health Outcomes
Authors: Miller Elizabeth , Lee Jessica Y. , DeWalt Darren A. , Vann William F. 
Published: June 2010

OBJECTIVE:
The objective of this study was to examine the relationship of primary caregivers' literacy with children's oral health outcomes.

METHODS:
We performed a cross-sectional study of children who were aged ≤6 years and presented for an initial dental appointment in the teaching clinics at the University of North Carolina at Chapel Hill School of Dentistry. Caregiver literacy was measured using the Rapid Estimate of Adult Literacy in Dentistry (REALD-30). The outcome measures included oral health knowledge, oral health behaviors, primary caregiver's reports of their child's oral health status, and the clinical oral health status of the child as determined by a clinical examination completed by trained, calibrated examiners.

RESULTS:
Among the 106 caregiver–child dyads enrolled, 59% of the children were male, 52% were white, and 86% of caregivers were the biological mothers. The bivariate results showed no significant relationships between literacy and oral health knowledge (P = .16) and behaviors (P = .24); however, there was an association between literacy and oral health status (P &lt; .05). The multivariate analysis controlled for race and income; this analysis revealed a significant relationship between caregiver literacy scores and clinical oral health status as determined by using a standardized clinical examination. Caregivers of children with mild to moderate treatment needs were more likely to have higher REALD-30 scores than those with severe treatment needs (odds ratio: 1.14 [95% confidence interval: 1.05–1.25]; P = .003).

CONCLUSIONS:
Caregiver literacy is significantly associated with children's dental disease status.
Preventive Care at Home for Very Preterm Infants Improves Infant and Caregiver Outcomes at 2 Years
Authors: Spittle Alicia J. , Anderson Peter J. , Lee Katherine J. , Ferretti Carmel , Eeles Abbey , Orton Jane , Boyd Roslyn N. , Inder Terrie , Doyle Lex W. 
Published: June 2010

OBJECTIVE:
The objective of this study was to determine the effects of preventive care at home on child development and primary caregiver mental health at 2 years of age.

METHODS:
A total of 120 very preterm infants (&lt;30 weeks) were assigned randomly to intervention (n = 61) or control (n = 59) groups. The intervention group received the preventive care program (9 home visits over the first year from a physiotherapist and a psychologist, focusing on the parent-infant relationship, the parents' mental health, and the infant's development); and the control group received standard care. At corrected age of 2 years, developmental outcomes were assessed, and primary caregivers completed the Infant-Toddler Social and Emotional Assessment. The mental health of the primary caregivers was assessed with the Hospital Anxiety and Depression Scale.

RESULTS:
At 2 years of age, 115 children (96%) were assessed with the Bayley Scales of Infant and Toddler Development III and 100 children (83%) with the Infant-Toddler Social and Emotional Assessment; and 91 (86%) of 106 caregivers completed the Hospital Anxiety and Depression Scale. There were no statistically significant differences in cognitive, language, or motor composite scores between the treatment groups. However, children in the intervention group were reported by their primary caregivers to exhibit less externalizing and dysregulation behaviors and increased competence, compared with control subjects. Primary caregivers in the intervention group reported less anxiety and depression.

CONCLUSION:
A preventive care program for very preterm infants and their families improved behavioral outcomes for infants and reduced anxiety and depression for primary caregivers.
Short-Term Overfeeding May Induce Peripheral Insulin Resistance Without Altering Subcutaneous Adipose Tissue Macrophages in Humans
Authors: Tam Charmaine S. , Viardot Alexander , Clément Karine , Tordjman Joan , Tonks Katherine , Greenfield Jerry R. , Campbell Lesley V. , Samocha-Bonet Dorit , Heilbronn Leonie K. 
Published: June 2010

OBJECTIVE
Chronic low-grade inflammation is a feature of obesity and is postulated to be causal in the development of insulin resistance and type 2 diabetes. The aim of this study was to assess whether overfeeding induces peripheral insulin resistance in lean and overweight humans, and, if so, whether it is associated with increased systemic and adipose tissue inflammation.

RESEARCH DESIGN AND METHODS
Thirty-six healthy individuals undertook 28 days of overfeeding by +1,250 kcal/day (45% fat). Weight, body composition, insulin sensitivity (hyperinsulinemic-euglycemic clamp), serum and gene expression of inflammation markers, immune cell activation, fat cell size, macrophage and T-cell numbers in abdominal subcutaneous adipose tissue (flow cytometry and immunohistochemistry) were assessed at baseline and after 28 days.

RESULTS
Subjects gained 2.7 ± 1.6 kg (P &lt; 0.001) and increased fat mass by 1.1 ± 1.6% (P &lt; 0.001). Insulin sensitivity decreased by 11% from 54.6 ± 18.7 to 48.9 ± 15.7 μmol/(kg of FFM)/min (P = 0.01). There was a significant increase in circulating C-reactive protein (P = 0.002) and monocyte chemoattractant protein-1 (P = 0.01), but no change in interleukin-6 and intercellular adhesion molecule-1. There were no changes in fat cell size, the number of adipose tissue macrophages or T-cells, or inflammatory gene expression and no change in circulating immune cell number or expression of their surface activation markers after overfeeding.

CONCLUSIONS
Weight gain-induced insulin resistance was observed in the absence of a significant inflammatory state, suggesting that inflammation in subcutaneous adipose tissue occurs subsequent to peripheral insulin resistance in humans.
Extraction of trace acetylspiramycin in real aqueous environments using aqueous two-phase system of ionic liquid 1-butyl-3-methylimidazolium tetrafluoroborate and phosphate
Authors: Wang Yun , Han Juan , Xie Xue-qiao , Li Chun-xiang 
Published: June 2010

Abstract
The partitioning of acetylspiramycin was carried out in an aqueous two-phase system (ATPS) formed by a hydrophilic ionic liquid (1-butyl-3-methylimidazolium tetrafluoraborate, [Bmim]BF4) and NaH2PO4. This ATPS is a simple, non-toxic and effective sample pretreatment technique, which was developed for the simultaneous separation, enrichment and rapid analysis of acetylspiramycin coupled with molecular fluorescence spectrophotometry. Analysis of the liquid-liquid equilibrium of [Bmim]BF4-salt ATPS demonstrated that the salting-out ability of different salts may be related to the Gibbs energy of hydration of the ions. The effects of types of salts, concentration of NaH2PO4, and temperature were analysed. Under optimum conditions, the average extraction efficiency and partition coefficient were 90.14% and 91.1, respectively. Thermodynamic functions provide some information about the molecular mechanism involved in acetylspiramycin transfer to the top phase, suggesting an important acetylspiramycin-[Bmim]BF4 interaction. The method yielded a linear range in the concentration from 1.0 to 10.0 µg mL−1 of acetylspiramycin, and the limit of detection was 0.02 µg mL−1. This method could be successfully applied for the analysis of acetylspiramycin in lake water, river water and groundwater. The proposed extraction technique appears to be suitable as a first step for the separation of macrolide antibiotics from real aqueous environments.
On the analytic properties of the S-matrix for the unknown interactions surrounded by centrifugal and rapidly decreasing potentials
Authors: Olkhovsky Vladislav 
Published: June 2010

Abstract
The analytic structure of the non-relativistic unitary and non-unitary S-matrix is investigated for the cases of the unknown interactions with the unknown motion equations inside a sphere of radius a, surrounded by the centrifugal and rapidly decreasing (exponentially or by the Yukawian law or by the more rapidly decreasing) potentials. The one-channel case and special examples of many-channel cases are considered. Some kinds of symmetry conditions are imposed. The Schroedinger equation for r > a for the particle motion and the condition of the completeness of the correspondent wave functions are assumed. The connection of the obtained results with the usual (temporal) causality is examined. Finally a scientific program is presented as a clear continuation and extension of the obtained results.
Beam deflection and splitting using transformation optics
Authors: Yu Guan , Jiang Wei , Cui Tie 
Published: June 2010

Abstract
We propose a scheme to achieve beam defecting and splitting using a method of embedded coordinate transformation [1]. In this method one boundary of transformation region is not involved in the transformation, which means beam defecting and splitting can be realized by changing the geometric construction. Although there is a discontinuous boundary, the impendence is matched between transformation media and surrounding media according to the conventional transformation optics. Full-wave numerical simulations further verify the theoretical analysis.
Th and U fission barriers within the Woods-Saxon two center shell model
Authors: Mirea Mihail , Tassan-Got Laurent 
Published: June 2010

Abstract
Fission barriers of actinides are calculated in the framework of the macroscopic-microscopic method. The single particle energies are obtained within a new version of the Woods-Saxon two-center shell model. A nuclear shape parametrization characterized by five degrees of freedom is used. The barriers are calculated along the minimal action trajectory in the configuration space and the inertia is evaluated within the cranking formalism. The reliability of the model is tested by comparing the theoretical results with values deduced from experimental data.
Vegetative growth of Umbrella Leaf Palm, <i>Johannesteijsmannia lanceolata</i> in Angsi Forest Reserve, Malaysia
Authors: Rozainah M. Z. , Sinniah U. R. 
Published: June 2010

A study of an acaulescent palm, Johannesteijsmannia lanceolata J. Dransfield was carried out in Angsi Forest Reserve Negeri Sembilan Malaysia for a period of 19 months A total of 32 adults, 24 juveniles and 32 seedlings from 3 different plots were censused every two weeks. The results showed that the average numbers of new leaves entering the crown with in the study period (19months) were: 3.3, 2.6 and 1.3 for 2.1, 1.6 and 0.8 leaves per year for adult juvenile and seedling, respectively. From the calculation it was discovered that the time spent by each leaf in the crown before it died was 8.8 years for adult and 8.4 years for juvenile. Key words: Johannesteijsmannia; Growth rate; Vegetative phenology; Arecaceae doi: 10.3126/eco.v12i0.3190 Ecoprint: An International Journal of Ecology 12: 11-20, 2005
Deleterious phases resulting from the induction bending of thick-walled super-duplex pipework
Authors: Collie Graeme , Black Ian , Byrne Glynn , Francis Roger 
Published: June 2010

Abstract
Temper free induction bending can have a significant impact on the mechanical and corrosion-resistance properties of thick-walled super duplex pipe. Sections from induction bent pipe were solution annealed at 1 120 °C for various periods to sequentially eliminate different deleterious phases. By comparing the results of modified DL-EPR analysis, optical microscopy, mechanical and corrosion testing before and after the various heat treatments, it was shown that the deterioration in properties was due to the precipitation of α′. It was also possible to determine the order in which α′, γ2 and Cr2N had precipitated, and thus to conclude that the unfavourable metallurgy was the result of inadequate post-bend cooling. A likely thermal history for the pipe is proposed that leads to the description of a modified temper free process.
Investigation of the atomic structure of molten As–Se alloys using X-ray diffraction
Authors: Knott Sabine , Gruner Sascha , Krone René , Marczinke Jennifer , Mikula Adolf , Hoyer Walter 
Published: June 2010

Abstract
An X-ray diffraction study on liquid As – Se alloys is reported. The average co-ordination number and the mean distance of nearest neighbour atoms obtained from the diffraction experiments and their relation to the composition of the alloy are in good agreement with earlier neutron diffraction results. After applying a model of micro-heterogeneous alloys it is suggested that As – Se alloys with less than 40 at.% As consist of structural units of As40Se60 stoichiometry in a matrix of Se-like structure. An assumption on the local arrangement of the nearest neighbours is deduced.
A theoretical approach to the elastic behaviour of compact and hollow spherical particles reinforced metal-matrix composites
Authors: Botas José D. , Velhinho Alexandre , Silva Rui J. C. 
Published: June 2010

Abstract
Current technology provides means of fabrication of spherical micro-particles, either hollow or compact, for all sorts of engineering materials. Such spherical particles can be further embedded into another material to build up either random dispersions or close-packed arrays, according to the production route and the degree of anisotropy intended for the ultimate composite material. In this study, a simple analytical formula for the composite stiffness is derived from an early micromechanics model to describe the actual reinforcement of ductile matrices by a random dispersion of uniform spherical ceramic particles. Predictions from this model are checked against some other relevant models, and specific features arising from its theoretical derivation are pointed out. The basic model can also predict the stiffness of syntactic composites, whose reinforcements are hollow microsphere dispersions. An application of this new model is demonstrated for the assessment of the ductility of brittle composites reinforced by compact spherical particles.
Evaluation of neck pain by using a visual analog scale before and after laminoplasty in patients with cervical myelopathy: relationship with clinical results
Authors: Ara Tsuyoshi , Iizuka Haku , Sorimachi Yasunori , Iizuka Yoichi , Nakajima Takashi , Nishinome Masahiro , Tsutsumi Satoshi , Takagishi Kenji 
Published: June 2010

Object
In this study the authors investigated the neck pain of patients with cervical myelopathy by using a visual analog scale (VAS) before and after laminoplasty, and they analyzed the association of amount of neck pain with the clinical results.

Methods
A retrospective review was conducted in 41 patients with cervical myelopathy who underwent cervical laminoplasty. The patients were assessed using questionnaires to evaluate the neck pain intensity before surgery, and 2 years after surgery, the outcome was assessed using a VAS. The degree of cervical lordosis and range of motion (ROM) of the cervical spine were evaluated before and after laminoplasty. The neurological status was also evaluated before and after surgery.

Results
The patients were classified into 2 groups according to their preoperative neck pain: 1) the pain (PA) group, which included patients whose preoperative VAS score was more than 1 mm; and 2) the no pain (NP) group, which included patients whose preoperative VAS score was 0 mm. Inclusion in the PA group indicated a restriction of the cervical ROM before laminoplasty; however, the improvement of neck pain in this group and the deterioration of pain status in the NP group eliminated this difference after laminoplasty. Thereafter, the PA group was classified into 2 subgroups according to the improvement of the preoperative neck pain: 1) the improved group, which included patients whose postoperative VAS score decreased; and 2) the no improvement group, which included patients who were not in the improved group. No significant differences were observed in the average recovery and radiographic results between these 2 subgroups.

Conclusions
Neck pain before surgery in the PA group indicated a restriction of the cervical ROM; however, the improvement of neck pain in this group and the deterioration of pain status in the NP group indicated the disappearance of this difference postoperatively. Moreover, improvement of preoperative neck pain was not associated with the radiographic results and the neurological recovery rate.