bluuebunny/crossref_metadata_2025_split huggingface dataset:
Guide for Aquifer Recharge Through Deep Injection Wells in Karst Environments: Case Study Los Elizondo Gallery, Mexico
Authors: ahuja gerardo augusto montemayor 
Published: June 2024

Abstract

The challenges posed by the various factors that affect the loss of surface and underground aquifers worldwide,
specifically in Mexico, are increasingly severe. This has forced scientists and technicians to look for alternatives
to conserve, sustain, and ensure the sustainability of liquid deposits, whether surface or underground in nature.
Based on the above, it was proposed to evaluate the technical and technological feasibility of constructing a
well for the artificial injection of water into the subsoil in the Los Elizondo Gallery in the Huajuco area. The
objective was to provide a technical and technological guide for the planning, design, and development of
groundwater conservation by deep injection. The methodology corresponded to a feasible project, in which the
procedures for the construction of the artificial water injection well to underground causes were followed. The
technical and technological feasibility of the construction of the well was evaluated, including the examination
of the geological and hydrological conditions of the area, as well as the available technologies and materials
that can be used to build the well. The execution of the project led to the determination that the construction of
a well for the artificial injection of water into the subsoil is technically and technologically feasible in the area
of the Los Elizondo Gallery. Once the appropriate procedures were applied, the Guide for aquifer recharge
through deep injection wells in karst environments was developed: a case study Los Elizondo Gallery, Mexico.
Road-associated variation in insect abundance differs between common orders
Authors: Sempe Suzan , Sole Catherine , Haussmann Natalie 
Published: June 2024

Abstract

The ecological impacts of roads are well-researched for many vertebrates, but studies are relatively lacking with regards to invertebrates. Here, we document changes in the abundance of ground-dwelling species of the three most common insect orders, Hymenoptera (specifically ants), Hemiptera (true bugs) and Coleoptera (beetles), with distance from a gravel road in a grassland system in South Africa. Insects were collected by means of 164 pitfall traps installed at 2, 5, 10 and 20 m perpendicular to a gravel road, and abundances of these three orders were compared statistically between the four distances. Whereas no differences in the numbers of Hymenoptera and Coleoptera were observed with distance from road, the abundance of Hemiptera was greater closer to the road. Although contrary to our initial expectation, this finding is in line with previous studies, which explore changes in plant chemistry and reduced predator efficiency next to roads as possible mechanisms. We conclude that not only busy, high-traffic roads should be taken into account in roadside ecological studies on insects, but that quieter, low-traffic roads should also be considered, as these can also affect the distribution of species at finer spatial scales.
Policy and process for returning raw genomic data to parents and young adult participants in a pediatric cancer precision medicine trial.
Authors: Barlow-Stewart Kristine , Barlow-Stewart Kristine , Courtney Eliza , Cowley Mark , Ebzery Camron , Bolanos Noemi Fuentes , Gifford Andrew , Harden Hazel , Josephi-Taylor Sarah , Kotecha Rishi , Mateos Marion , Manzur Mitali , Mayoh Chelsea , Milnes Dianne , Nielsen Jane , O'Connor Matthew , Padhye Bhavna , Pitman Catherine , Pitman Elizabeth , Pinese Mark , Speechly Catherine , Sullivan Ashleigh , Trahair Toby , Tucker Katherine , Tyrrell Vanessa , Warby Meera , Wood Andrew , Ziegler David , Johnston Carolyn 
Published: June 2024

Abstract

Sharing raw genomic data with research participants is not universally mandated. Yet, in padiatric cancer precision medicine clinical trials settings, parents proactively seeking treatment and answers to causation may request return of their child’s raw data and/or biospecimen. To satisfy such requests with ethical, timely and appropriate responses, the ZERO Childhood Cancer Program required development of a Policy and Process document. Development occurred iteratively: V1 from the literature; V2-4 following a modified Delphi expert consultation over three Rounds; V5-6 following two rounds of consumer consultation; and a final ethically approved V7: Policy (purpose; background; ethical considerations), Process (nine steps), leaflet for those making a request and consent form. Twenty-one Delphi experts (71% pediatric oncologists/hematologists, clinical geneticists/genetic counsellors – most likely involved with requests) achieved consensus (> 80%) by Round 3. Issues addressed included broadening ethical considerations (autonomy/child’s best interests, beneficence/clinical utility, non-maleficence, reciprocity, responsibility to genetic relatives); no obligation on treating clinicians to act on therapeutic recommendations arising; limiting practical barriers to return by streamlining the process. Consumer consultations highlighted need for expected turnaround times, given no reportable or actionable findings may trigger requests; acknowledging potential great value of subsequent analysis/interpretation; and supporting parental empowerment by facilitating meeting with a study genetic counselor (or equivalent), separate from their treating clinician, if preferred, to manage their request. Informed by expert consensus and consumer views, this guide to fulfilling data sharing requests, in a spirit of reciprocity between researchers and participants/parents/guardians, may be a model for other research groups and inform ethical guidelines.
Phytoremediation potential of forage grasses in copper-contaminated environments
Authors: Daniel Eduardo da Silva , Rosini Daniely Neckel , Winter Fabio Luis , Silva Andrei de Souza da , Sbrissia André Fischer , Primieri Silmar , CAMPOS MARI LUCIA 
Published: June 2024

Abstract

Human activities have considerably increased copper (Cu). This study evaluated the potential of copper phytoextraction in competing and resource-conserving plants. The experiment was carried out in a greenhouse with eight tropical forage grasses, at two levels of Cu in the nutrient solution: 0.3 and 20 µmol L− 1. Variables of plant morphogenesis, leaf area measurements, SPAD index, total length, area, volume and average diameter of roots, specific leaf area, specific root area, root density, root length density, specific root length were evaluated, and the relationship between leaf and stem and roots and shoots. Data were submitted to analysis of variance and test of means. Forage grasses did not maintain their functional groups at the highest copper concentrations. The promising species for phytoremediation of copper contaminated areas were marandu, piatã and giant missioneira grass.
Comparison of WASH with Flood Perception among Rural and Urban Communities in Kota Tinggi, Malaysia.
Authors: Hamid Aminiril Anisah Abdul , Kwa Yee Chu , Suboh Norlilawati , Sing Cindy Lee Ik , Wei Dominic Kay Shuen , Walsh Claire , Goodson Michaela Louise 
Published: June 2024

Abstract

The United Nations aimed to provide safe drinking water, sanitation, and hygiene (WASH) around the world, but flooding in Kota Tinggi, Johor, Malaysia, may cause the country to miss its population coverage target of 92–97% by 2030. Due to limited access to clean water and sanitation, Kota Tinggi's rural communities faced significant WASH challenges, resulting in the spread of waterborne diseases. Meanwhile, worsening flooding in Kota Tinggi's town area may cause damage to WASH infrastructure, disrupting clean water and sanitation services in urban areas. The study sought to compare WASH provision in rural and urban areas, as well as to identify knowledge, attitudes, and behaviours concerning flooding, flood prevention, and water-related infectious diseases. We surveyed 30 and 50 households from rural and urban areas in Kota Tinggi respectively. The findings revealed that both communities practiced safe drinking water, but at varying levels of sanitation facility availability, with urban areas proving to be more prosperous than rural areas. There was a significant difference in flood knowledge and perceived flood risk between rural and urban communities, resulting in different levels of flood preparedness. To summarize, improving WASH provision and flood preparedness in Kota Tinggi requires a tailored approach that addresses the specific needs of both rural and urban communities.
Changes of nestling ringing dates in nine bird species over seven decades
Authors: Reiczigel Jenő , Erős Nándor , Szabó Alfréd , Vadas Anita-Bernadett , Rózsa Lajos 
Published: June 2024

Abstract

Climate change co-occurs with an advancement of avian breeding season (indexed as laying dates or fledging dates) in the temperate zone, suggesting a causality between them. Here, we investigate whether the long-term shifts in nestling (chick) ringing dates also mirror this phenomenon. This index is biased by inherent shortcomings, such as the non-independence of dates (in nestmates, colony members), poor accuracy (long period suitable for ringing), and strange shape of distributions. These shortcomings can be reduced by applying the median of annual ringing dates as an index of breeding phenology. The advantage of this index is that data are available for long periods and large sample sizes. By accepting certain compromise between statistical discipline and fieldwork realities, we examined changes in the breeding phenology of 9 bird species from 1951 to 2020 in Hungary. We found that the annual median of ringing dates advanced significantly (by 9–14 days) in the Black-headed Gull, Common Kestrel, Barn Swallow, Great Tit, and Eurasian Blue Tit. Contrarily, no significant (all P > 0.16) changes occurred in the case of the Common Tern, Black-crowned Night-heron, Common Buzzard, and Long-eared Owl. We also found that the proportion of Great Tits’ second brood has been reduced in recent decades.
Epidemiological characteristics and diagnosis and treatment effect of rifampicin-resistant pulmonary tuberculosis (RR-PTB) in Guizhou province
Authors: Zhou Jian , Li Jinlan , Hu Yong , Li Shijun 
Published: June 2024

Abstract

Background
Rifampicin-resistant pulmonary tuberculosis (RR-PTB) presents a significant threat to global public health security. China bears a substantial burden of RR-PTB cases globally, with Guizhou Province experiencing particularly alarming trends, marked by a continual increase in patient numbers. Understanding the population characteristics and treatment modalities for RR-PTB is crucial for mitigating morbidity and mortality associated with this disease.
Methods
We gathered epidemiological, diagnostic, and treatment data of all RR-PTB cases recorded in Guizhou Province from 2017 to 2023. Utilizing composition ratios as the analytical metric, we employed Chi-square tests to examine the spatiotemporal distribution patterns of RR-PTB patients and the evolving trends among different patient classifications over the study period.
Results
In our study, 3396 cases of RR-PTB were analyzed, with an average age of 45 years. The number of RR-PTB patients rose significantly from 176 in 2017 to 960 in 2023, peaking notably among individuals aged 23–28 and 44–54, with a rising proportion in the 51–80 age group (P < 0.001). Since 2021, there has been a notable increase in the proportion of female patients. While individuals of Han nationality comprised the largest group, their proportion decreased over time (P < 0.001). Conversely, the Miao ethnicity showed an increasing trend (P < 0.05). The majority of patients were farmers, with their proportion showing an upward trajectory (P < 0.001), while students represented 4.33% of the cases. Geographically, most patients were registered in Guiyang and Zunyi, with a declining trend (P < 0.001), yet household addresses primarily clustered in Bijie, Tongren, and Zunyi. The proportion of floating population patients gradually decreased, alongside an increase in newly treated patients and those without prior anti-tuberculosis therapy. Additionally, there was a notable rise in molecular biological diagnostic drug sensitivity (P < 0.001). However, the cure rate declined, coupled with an increasing proportion of RR-PTB patients lost to follow-up and untreated (P < 0.05).
Conclusions
Enhanced surveillance is crucial for detecting tuberculosis patients aged 23–28 and 44–54 years. The distribution of cases varies among nationalities and occupations, potentially influenced by cultural and environmental factors. Regional patterns in RR-PTB incidence suggest tailored prevention and control strategies are necessary. Despite molecular biology advances, challenges persist with low cure rates and high loss to follow-up. Strengthening long-term management, resource allocation, and social support systems for RR-PTB patients is essential.
Clinical Characteristics and Prognosis of Patients with Follicular Lymphoma Grade 3A: A real-world study in a single centre
Authors: Ye Xingnong , Xu Gaixiang , Li Xia , Wei Juying , Zhang Xuewu , Zhang Xiang , Zhu Yanan , Lv Yunfei , Xiao Feng , Yang Chunmei , Zhou De , Xie Wanzhuo , Huang Jian , Lou Yinjun , Mao Liping , Yang Min , Mai Wenyuan , Meng Haitao , Jin Jie , Yu Wenjuan , Tong Hongyan 
Published: June 2024

Abstract

Purpose Follicular lymphoma (FL) is common subtype of indolent non-Hodgkin's lymphoma (NHL). However, there is no consensus on the management of FL grade 3A (FL3A). Methods We performed a real-world study of newly diagnosed FL patients from January 2013 to December 2022. we collected the clinical data of FL3A patients to analyse the correlation among baseline features, therapy regimens and prognosis. The data were collected from the hospital's electronic medical records system. Results A total of 223 patients with FL3A were enrolled. With a median follow-up of 41 months, the expected 5-year overall survival (OS) was 97.4% and the 5-year progression-free survival (PFS) was 73%. In real-word, most patients with advanced FL3A in low-tumor-load received therapy, majority with RCHOP regimen (rituximab, cyclophosphamide, doxorubicin, vincristine, prednisone). Patients with advanced FL3A treated with RCHOP regimen and maintenance therapy had better PFS. There was no significant difference in PFS between the treatment group and watch-and-wait group in patients with low-tumor-load. The univariate analyses indicated that the maximum 18FFluorodeoxyglucose uptake in PET (SUVmax), Ki-67 index, platelet count were related to prognosis. Multivariate analyses showed that only SUVmax was the independent prognostic factor and SUVmax ≥ 15 related with poor PFS. Conclusion FL3A patients have a long survival, with a 5-year PFS of 73%. In real-world, most patients with advanced FL3A in low-tumor-load received therapy. Multivariate analyses indicated that SUVmax ≥ 15 was an independent poor prognostic factor affecting PFS in patients with advanced FL3A. In addition, Ki-67 index was also maybe related with prognosis.
Video Observed Therapy Device Improves Probability of Tuberculosis Therapy Adherence; A Randomised Clinical Trial at a TB Reference Clinic in Lagos
Authors: Amoo Olufemi , Kareithi Dorcas , Tijani Bosun , Onuigbo Tochukwu , Karera Steven , Oladejo Busayo , Oraegbu Joy , Ezike Jennifer , Adekoya Adepeju , Sani Maryam , David Nkiru , Audu Rosemary , Salako Babatunde 
Published: June 2024

Abstract

Background
Video Observed Therapy (VOT) is a method of adherence monitoring where participants transmit digital images of their treatment intake to a central location for review; either synchronously or asynchronously. VOT presents an option that can be explored for developing countries like Nigeria with a high TB burden, thereby reducing the TB burden and ultimately eliminating it.
Methods
The study was a two-arm individually randomised clinical trial conducted by Nigeria Institute of Medical Research (NIMR) in Lagos, Nigeria. Participants (100) were recruited to this study and randomised into either treatment (VOT) or DOT (Directly Observed Therapy) as control. Data were collected through the NimCure mobile app and participant records. We compared treatment outcomes between VOT and DOT and assessed average treatment effects by adjusting for gender, location (state), and age.
Results
A high rate of 100% adherence to TB treatment was achieved in VOT (97%) and DOT (79%). More females than males achieved 100% adherence to VOT, while more males achieved 100% adherence to DOT. Rural dwellers achieved 100% adherence to both VOT and DOT. There was a varied distribution across all age groups. The average adherence, if all participants were to use VOT, would be 0.1709 (z = 2.23, p = 0.026) more than the average that would be if the participants did not use VOT.
Conclusion
VOT through the NimCure application achieved the adherence objective and showed that VOT can be used for effective remote monitoring and management of Tuberculosis in Nigeria. NimCure can therefore be recommended for use in low-income settings.
Unraveling the dynamics of host specificity and resistance responses to potato virus Y, and implications for crop management
Authors: de Morais Ivair José , Silva Dorian Yest Melo , Camargo Barbara Mavie , Lourenção André Luiz , Inoue-Nagata Alice Kazuko 
Published: June 2024

Abstract

Potato virus Y (PVY), a virus member of the family Potyviridae, poses a significant threat to global agriculture, affecting crops such as potato, tomato, pepper, and tobacco. Despite its economic importance, there remains a critical gap in understanding the dynamics of PVY-host interactions and the development of effective management strategies. This study aimed to comprehensively characterize PVY isolates from sweet pepper, potato, and tomato plants, elucidating their infectivity and adaptation across diverse host species and cultivars. Initially, using antigen-trapped ELISA, we determined the optimal detection timeframe and leaf sampling strategy for detection of PVY by serological assays, showing that some hosts require a minimum incubation period and leaf selection for a reliable virus detection. By comparing PVY isolates from distinct hosts, we demonstrate that the choice of the isolate is crucial for resistance evaluations. Additionally, inoculation trials across various plant species elucidated differences in infectivity and adaptation among PVY isolates. Resistance trials in commercial cultivars of tomato and pepper plants and wild Solanum spp. accessions revealed susceptibility across all tested materials, challenging previous assumptions of resistant cultivars and accessions. These findings underscore the urgency of addressing PVY spread and understanding host-virus interactions to identify resistant genotypes for commercial use and for breeding programs directed to PVY isolates present in Brazil.
Genome-wide analysis of the common bean (Phaseolus vulgaris) laccase gene family and its functions in response to abiotic stress
Authors: Cheng Tong , Ren Chunyuan , Xu Jinghan , Wang Huamei , Wen Bowen , Zhao Qiang , Zhang Wenjie , Yu Gaobo , Zhang Yuxian 
Published: June 2024

Abstract

Background
Laccase (LAC) gene family plays a pivotal role in plant lignin biosynthesis and adaptation to various stresses. Limited research has been conducted on laccase genes in common beans.
Results
29 LAC gene family members were identified within the common bean genome, distributed unevenly in 9 chromosomes. These members were divided into 6 distinct subclades by phylogenetic analysis. Further phylogenetic analyses and synteny analyses indicated that considerable gene duplication and loss presented throughout the evolution of the laccase gene family. Purified selection was shown to be the major evolutionary force through Ka / Ks. Transcriptional changes of PvLAC genes under low temperature and salt stress were observed, emphasizing the regulatory function of these genes in such conditions. Regulation by abscisic acid and gibberellins appears to be the case for PvLAC3, PvLAC4, PvLAC7, PvLAC13, PvLAC14, PvLAC18, PvLAC23, and PvLAC26, as indicated by hormone induction experiments. Additionally, the regulation of PvLAC3, PvLAC4, PvLAC7, and PvLAC14 in response to nicosulfuron and low-temperature stress were identified by virus-induced gene silence, which demonstrated inhibition on growth and development in common beans.
Conclusions
The research provides valuable genetic resources for improving the resistance of common beans to abiotic stresses and enhance the understanding of the functional roles of the LAC gene family.
Pruritus and xerodermia in patients treated with checkpoint inhibitors for melanoma, lung and renal cancer: a meta-analysis
Authors: Kiss Igor , Svoboda Michal , Horska Cecilie , Buchler Samuel , Buchler Tomas 
Published: June 2024

Abstract

Background: Dermatologic adverse events (DAEs) are common in patients treated with immune checkpoint inhibitors (ICI) for cancer.
Objective: To evaluate the incidence of two selected dermatologic adverse events (DAEs), pruritus and xerodermia, reported as adverse events in phase 3 randomised clinical trials using ICI in patients with lung cancer, renal cancer, and melanoma.
Methods: Phase 3 studies where treatment included an ICI in the experimental arm were analysed. For pruritus, 42 studies were analysed with a total of 29,502 evaluable patients. For xerodermia, the number of studies was 23 with 17,341 patients.
Results:. Pruritus was associated with ICI therapy with OR of 3.02 (95% CI 2.57–3.55). Compared to renal cell carcinoma (RCC) treated with anti-programmed death (PD-1) agents, patients with melanoma had higher odds of reporting pruritus, reaching statistical significance for the anti-PD-1 and anti-cytotoxic T-lymphocyte associated protein 4 (CTLA4) treatment. Patient with lung cancer had significantly lower odds of pruritus across all ICI types compared to other analysed tumour types. Xerodermia was also associated with ICI treatment. Using RCC/PD-1 as a reference, there was a trend to higher incidence of xerodermia in patients with melanoma. Patients with lung cancer had significantly lower odds of xerodermia regardless of ICI type.
Conclusion: The present systematic analysis indicates that pruritus and xerodermia are associated with ICI treatment of any type compared to non-immunologic therapies. There appears to be a higher incidence of these DAEs in patients with melanoma and RCC compared to patients with lung cancer.