bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neoproterozoic extension in the Greater Dharwar Craton: a reevaluation of the “Betsimisaraka suture” in MadagascarThis article is one of a series of papers published in this Special Issue on the theme ofGeochronologyin honour of Tom Krogh.
Authors: Tucker R. D. , Roig J.-Y. , Delor C. , Amelin Y. , Goncalves P. , Rabarimanana M. H. , Ralison A. V. , Belcher R. W. 
Published: February 2011

The Precambrian shield of Madagascar is reevaluated with recently compiled geological data and new U–Pb sensitive high-resolution ion microprobe (SHRIMP) geochronology. Two Archean domains are recognized: the eastern Antongil–Masora domain and the central Antananarivo domain, the latter with distinctive belts of metamafic gneiss and schist (Tsaratanana Complex). In the eastern domain, the period of early crust formation is extended to the Paleo–Mesoarchean (3.32–3.15 Ga) and a supracrustal sequence (Fenerivo Group), deposited at 3.18 Ga and metamorphosed at 2.55 Ga, is identified. In the central domain, a Neoarchean period of high-grade metamorphism and anatexis that affected both felsic (Betsiboka Suite) and mafic gneisses (Tsaratanana Complex) is documented. We propose, therefore, that the Antananarivo domain was amalgamated within the Greater Dharwar Craton (India + Madagascar) by a Neoarchean accretion event (2.55–2.48 Ga), involving emplacement of juvenile igneous rocks, high-grade metamorphism, and the juxtaposition of disparate belts of mafic gneiss and schist (metagreenstones). The concept of the “Betsimisaraka suture” is dispelled and the zone is redefined as a domain of Neoproterozoic metasedimentary (Manampotsy Group) and metaigneous rocks (Itsindro–Imorona Suite) formed during a period of continental extension and intrusive igneous activity between 840 and 760 Ma. Younger orogenic convergence (560–520 Ma) resulted in east-directed overthrusting throughout south Madagascar and steepening with local inversion of the domain in central Madagascar. Along part of its length, the Manampotsy Group covers the boundary between the eastern and central Archean domains and is overprinted by the Angavo–Ifanadiana high-strain zone that served as a zone of crustal weakness throughout Cretaceous to Recent times.
Birthdate for the Coronation paleocean: age of initial rifting in Wopmay orogen, CanadaThis article is one of a series of papers published in this Special Issue on the theme of Geochronology in honour of Tom Krogh.
Authors: Hoffman Paul F. , Bowring Samuel A. , Buchwaldt Robert , Hildebrand Robert S. 
Published: February 2011

The 1.9 Ga Coronation “geosyncline” to the west of Slave craton was among the first Precambrian continental margins to be identified, but its duration as a passive margin has long been uncertain. We report a new U–Pb (isotope dilution – thermal ionization mass spectrometry (ID–TIMS)) 207Pb/206Pb date of 2014.32 ± 0.89 Ma for zircons from a felsic pyroclastic rock at the top of the Vaillant basalt, which underlies the passive margin sequence (Epworth Group) at the allochthonous continental slope. A sandstone tongue within the basalt yields Paleoproterozoic (mostly synvolcanic) and Mesoarchean detrital zircon dates, of which the latter are compatible with derivation from the Slave craton. In contrast, detrital zircon grains from the Zephyr arkose in the accreted Hottah terrane have Paleoproterozoic and Neoarchean dates. The latter cluster tightly at 2576 Ma, indistinguishable from igneous zircon dates reported here from the Badlands granite, which is faulted against the Vaillant basalt and underlying Drill arkose. We interpret these data to indicate that Badlands granite belongs to the hanging wall of the collisional geosuture between Hottah terrane and the Slave margin, represented by the Drill–Vaillant rift assemblage. If 2014.32 ± 0.89 Ma dates the rift-to-drift transition and 1882.50 ± 0.95 Ma (revised from 1882 ± 4 Ma) the arrival of the passive margin at the trench bordering the Hottah terrane, the duration of the Coronation passive margin was ∼132 million years, close to the mean age of extinct Phanerozoic passive margins of ∼134 million years (see Bradley 2008).
High-precision U–Pb geochronology of the Butedale pluton, British ColumbiaThis article is one of a series of papers published in this Special Issue on the theme of Geochronology in honour of Tom Krogh.
Authors: Denyszyn Steven W. , Mundil Roland , Brownlee Sarah J. , Renne Paul R. 
Published: February 2011

The Butedale pluton, a ca. 100 km long compositionally zoned batholith, is part of the Coast Plutonic Complex that extends the length of the Canadian Pacific coast. Its age and thermal history are relevant to the Butedale pluton’s role as a test case of the Baja–BC hypothesis, as paleomagnetic evidence suggests that it may have formed thousands of kilometres to the south and moved northward along what is now the Coast Shear Zone. High-resolution U–Pb (chemical abrasion – thermal ionization mass spectrometry (CA–TIMS), zircon) analysis of rocks across the width of the Butedale pluton indicates that it is actually made up of at least two distinct magmatic events that formed the West Butedale pluton (ca. 95 Ma) and the East Butedale pluton (ca. 85 Ma). The East Butedale pluton was reheated by the emplacement of a younger adjacent pluton, which may have caused partial Pb loss and resulting excess scatter of 206Pb/238U zircon ages within individual samples. The West Butedale pluton may be the same age as, and part of, the nearby Ecstall pluton, thereby doubling the length of the Ecstall pluton to ca. 200 km. Single-grain, high-precision U–Pb analysis of zircon reveals previously unknown complexity and detail of emplacement and thermal history in the Butedale plutons.
The Pikwitonei Granulite Domain, Manitoba: a giant Neoarchean high-grade terrane in the northwest Superior ProvinceThis article is one of a series of papers published in this Special Issue on the theme of Geochronology in honour of Tom Krogh.N. Machado, T.E. Krogh, and W. Weber are deceased.
Authors: Heaman L. M. , Böhm Ch. O. , Machado N. , Krogh T. E. , Weber W. , Corkery M. T. 
Published: February 2011

The Pikwitonei Granulite Domain located at the northwestern margin of the Superior Province is one of the largest Neoarchean high-grade terranes in the world, with well-preserved granulite metamorphic assemblages preserved in a variety of lithologies, including enderbite, opdalite, charnockite, and mafic granulite. U–Pb geochronology has been attempted to unravel the protolith ages and metamorphic history of numerous lithologies at three main localities; Natawahunan Lake, Sipiwesk Lake, and Cauchon Lake. The U–Pb age results indicate that some of the layered enderbite gneisses are Mesoarchean (3.4–3.0 Ga) and the more massive enderbites are Neoarchean. The high-grade metamorphic history of the Pikwitonei Granulite Domain is complex and multistage with at least four episodes of metamorphic zircon growth identified: (1) 2716.1 ± 3.8 Ma, (2) 2694.6 ± 0.6 Ma, (3) 2679.6 ± 0.9 Ma, and (4) 2642.5 ± 0.9 Ma. Metamorphic zircon growth during episodes 2 and 3 are interpreted to be regional in extent, corresponding to M1 amphibolite- and M2 granulite-facies events, respectively, consistent with previous field observations. The youngest metamorphic episode at 2642.5 Ma is only recognized at southern Cauchon Lake, where it coincides with granite melt production and possible development of a major northeast-trending deformation zone. The timing and multistage metamorphic history recorded in the Pikwitonei Granulite Domain is similar to most Superior Province high-grade terranes and marks a fundamental break in Archean crustal evolution worldwide at the termination of prolific global Neoarchean greenstone belt formation.
High-precision U–Pb age and geochemistry of the mineralized (Ni–Cu–Co) Suwar intrusion, YemenThis article is one of a series of papers published in this Special Issue on the theme of Geochronology in honour of Tom Krogh.
Authors: Greenough John D. , Kamo Sandra L. , Theny Lucia , Crowe Sean A. , Fipke Charles 
Published: February 2011

High-precision U–Pb isotope dilution – thermal ionization mass spectrometry (ID–TIMS) geochronology on chemically abraded zircon grains from a noritic gabbro of the Ni-bearing Suwar mafic–ultramafic layered complex, northwestern Yemen, gives a mean 206Pb/238U age of 638.46 ± 0.73 Ma (2σ; MSWD = 1.4). At Wadi Qutabah, ∼30 km to the north, a similar mafic sample has an identical age of 638.58 ± 0.51 Ma (2σ; MSWD = 0.32), which supports the possibility of there being a single, large intrusive complex with an estimated areal extent of ∼250 km2. This is supported by geochemical data of samples from each locality, which are postkinematic, gabbroic rocks that contain variable amounts of cumulus olivine, plagioclase, orthopyroxene, and ilmenite with intercumulus augite, hornblende, and Ni-sulphides. Straight rare Earth element (REE) patterns, Ba/La ∼30, Rb/Ba ∼0.04, and negative primitive-mantle-normalized P anomalies resemble EM1 (Enriched Mantle 1) of oceanic island basalts and Archean subcontinental mantle lithosphere. The mineralogy and magmatic/tectonic discrimination diagrams suggest within-plate continental tholeiitic and noritic cumulates typical of a rift setting for both intrusions. The complex intrudes retrograded, amphibolite-facies paragneiss of the Pan-African Afif lithotectonic terrane, and is generally undeformed and unaltered, and, therefore, unaffected by the Pan-African orogeny. Emplacement of the 639 Ma complex occurred during a tensional tectonic regime on the Arabian Peninsula and marks the time of proto-Iapetan rifting. The estimated size of the intrusion, its noritic composition, and Archean subcontinental lithospheric mantle signature and its position in thin Proterozoic lithosphere abutting Archean cratonic rocks, give it the key characteristics of known, mostly Proterozoic, intrusions that host world-class Ni–Cu–Co ore deposits.
Comparative analysis of fecal microbiota and intestinal microbial metabolic activity in captive polar bears
Authors: Schwab Clarissa , Gänzle Michael 
Published: February 2011

The composition of the intestinal microbiota depends on gut physiology and diet. Ursidae possess a simple gastrointestinal system composed of a stomach, small intestine, and indistinct hindgut. This study determined the composition and stability of fecal microbiota of 3 captive polar bears by group-specific quantitative PCR and PCR–DGGE (denaturing gradient gel electrophoresis) using the 16S rRNA gene as target. Intestinal metabolic activity was determined by analysis of short-chain fatty acids in feces. For comparison, other Carnivora and mammals were included in this study. Total bacterial abundance was approximately log 8.5 DNA gene copies·(g feces)–1in all 3 polar bears. Fecal polar bear microbiota was dominated by the facultative anaerobes Enterobacteriaceae and enterococci, and the Clostridium cluster I. The detection of the Clostridium perfringens α-toxin gene verified the presence of C. perfringens. Composition of the fecal bacterial population was stable on a genus level; according to results obtained by PCR–DGGE, dominant bacterial species fluctuated. The total short-chain fatty acid content of Carnivora and other mammals analysed was comparable; lactate was detected in feces of all carnivora but present only in trace amounts in other mammals. In comparison, the fecal microbiota and metabolic activity of captive polar bears mostly resembled the closely related grizzly and black bears.
Curcumin as a promising anticandidal of clinical interest
Authors: Neelofar K. , Shreaz S. , Rimple B. , Muralidhar S. , Nikhat M. , Khan Luqman A. 
Published: February 2011

Curcumin, an important Asian spice, is part of many Indian food preparations. This work evaluates the antifungal activity of curcumin against 14 strains of Candida (10 clinical and 4 standard). Curcumin displayed antifungal properties against all tested Candida strains, with minimum inhibitory concentrations (MICs) varying from 250 to 2000 µg·mL–1. The in vitro effect of curcumin on growth, sterol content, proteinase secretion, and H+ extrusion by plasma membrane ATPase was investigated for 2 standard strains Candida albicans ATCC 10261 and Candida glabrata ATCC 90030 and compared with the effect of fluconazole. At MIC, curcumin inhibited H+ extrusion in 2 species of Candida by 42% and 32% in the absence of glucose and by 28% and 18% in the presence of glucose. Respective inhibition of H+ extrusion caused by the MIC of fluconazole was 85% and 89% in the absence of glucose and 61% and 66% in its presence. Ergosterol content decreased by 70% and 53% for the 2 strains following exposure to curcumin at MIC; comparative values for fluconazole at MIC were 93% and 98%. Curcumin and fluconazole decreased proteinase secretion by 49% and 53%, respectively, in C. albicans and by 39% and 46%, respectively, in C. glabrata. In conclusion, curcumin is found to be active against all tested clinical and standard strains but is less effective than fluconazole. Antifungal activity of curcumin might be originating from alteration of membrane-associated properties of ATPase activity, ergosterol biosynthesis, and proteinase secretion.
An important role for secreted esterase in disease establishment of the wheat powdery mildew fungus Blumeria graminis f. sp.tritici
Authors: Feng Jie , Wang Feng , Hughes Geoff R. , Kaminskyj Susan , Wei Yangdou 
Published: February 2011

The activity of esterase secreted by conidia of wheat powdery mildew fungus, Blumeria graminis f. sp. tritici, was assayed using indoxyl acetate hydrolysis, which generates indigo blue crystals. Mature, ungerminated, and germinating conidia secrete esterase(s) on artificial media and on plant leaf surfaces. The activity of these esterases was inhibited by diisopropyl fluorophosphate, which is selective for serine esterases. When conidia were inoculated on wheat leaves pretreated with diisopropyl fluorophosphate, both appressorial germ tube differentiation and symptom development were significantly impaired, indicating an important role of secreted serine esterases in wheat powdery mildew disease establishment.
A Feasibility Study of Bevacizumab plus Dose-Dense Doxorubicin–Cyclophosphamide (AC) Followed by Nanoparticle Albumin–Bound Paclitaxel in Early-Stage Breast Cancer
Authors: McArthur Heather L. , Rugo Hope , Nulsen Benjamin , Hawks Laura , Grothusen Jill , Melisko Michelle , Moasser Mark , Paulson Matthew , Traina Tiffany , Patil Sujata , Zhou Qin , Steingart Richard , Dang Chau , Morrow Monica , Cordeiro Peter , Fornier Monica , Park John , Seidman Andrew , Lake Diana , Gilewski Theresa , Theodoulou Maria , Modi Shanu , D'Andrea Gabriella , Sklarin Nancy , Robson Mark , Moynahan Mary Ellen , Sugarman Steven , Sealey Jane E. , Laragh John H. , Merali Carmen , Norton Larry , Hudis Clifford A. , Dickler Maura N. 
Published: February 2011

Abstract
Purpose: Bevacizumab confers benefits in metastatic breast cancer but may be more effective as adjuvant therapy. We evaluated the cardiac safety of bevacizumab plus dose-dense doxorubicin–cyclophosphamide (ddAC) → nanoparticle albumin–bound (nab)-paclitaxel in human epidermal growth factor receptor 2 normal early-stage breast cancer.
Experimental Design: Eighty patients with normal left ventricular ejection fraction (LVEF) were enrolled. Bevacizumab was administered for 1 year, concurrently with ddAC → nab-paclitaxel then as a single agent. LVEF was evaluated at months 0, 2, 6, 9, and 18. This regimen was considered safe if fewer than three cardiac events or fewer than two deaths from left ventricular dysfunction occurred. Correlative studies of cardiac troponin (cTn) and plasma renin activity (PRA) were conducted.
Results: The median age was 48 years (range, 27–75 years), and baseline LVEF was 68% (53%–82%). After 39 months' median follow-up (5–45 months): median LVEF was 68% (53%–80%) at 2 months (n = 78), 64% (51%–77%) at 6 months (n = 66), 63% (48%–77%) at 9 months (n = 61), and 66% (42%–76%) at 18 months (n = 54). One patient developed symptomatic LV dysfunction at month 15. Common toxicities necessitating treatment discontinuation were hypertension (HTN, 4%), wound-healing complications (4%), and asymptomatic LVEF declines (4%). Neither cTn nor PRA predicted congestive heart failure (CHF) or HTN, respectively.
Conclusions: Bevacizumab with ddAC → nab-paclitaxel had a low rate of cardiac events; cTn and PRA levels are not predictive of CHF or HTN, respectively. The efficacy of bevacizumab as adjuvant treatment will be established in several ongoing phase III trials. Clin Cancer Res; 17(10); 3398–407. ©2011 AACR.
Abstract 1000: Rad6B induced β-catenin stabilization requires intact Wnt signaling
Authors: Gerard Brigitte , Tait Larry , Shah Seema , Nangia-Makker Pratima , Shekhar Malathy PV 
Published: February 2011

Abstract
Aberrant activation of Wnt signaling results from stabilization and transcriptional activation of β-catenin and is often associated with carcinogenesis. Clinical evidence suggests that β-catenin accumulation occurs in breast cancer although activating mutations are rare. We have previously shown that Rad6B positively regulates β-catenin stability and transcriptional activity in breast cancer cells by inducing stable polyubiquitin modifications. Here we show that Rad6B physically interacts with β-catenin within the β-catenin amino acid segment containing the first armadillo repeat, which is also a binding site for casein kinase 1 (CK1). β-catenin phosphorylation by GSK3β and subsequent ubiquitination and proteasomal degradation is dependent upon CK1-mediated initiating phosphorylation of β-catenin at Ser45. In vitro kinase assays showed that Rad6B inhibits Ser45 phosphorylation by CK1, and Ser45 phosphorylated β-catenin is decreased in Rad6B overexpressing breast cancer cells. In vitro and in vivo mapping, and site-directed mutation analysis identified K394 of β-catenin as the predominant site for ubiquitination by Rad6B. MCF-7 breast cancer cells engineered to express myc-tagged K394R-β-catenin showed decreased steady-state levels and β-catenin transcriptional activity as compared to cells expressing wild type β-catenin. We also determined whether Rad6B-mediated β-catenin stabilization occurs independently of the canonical Wnt signaling pathway. MDA-MB-231 breast cancer cells overexpressing endogenous Rad6B were isolated on the basis of Rad6B promoter activity, and the consequences of Wnt disruption on β-catenin levels, transcriptional activity and Rad6B expression were determined by expressing a dominant negative mutant of Wnt coreceptor, LRP6. Disruption of Wnt signaling inhibited Rad6B expression and β-catenin accumulation. Both Wnt signaling disruption and Rad6B silencing separately enhanced localization of β-catenin on the cell membranes and decreased intracellular β-catenin accumulation. In summary, our data suggest that the Rad6B overexpression frequently observed in breast carcinomas contribute to β-catenin stabilization and transcriptional activation by inhibiting the CK1-mediated initiating phosphorylation and by ubiquitinating K394. However, the Rad6B induced β-catenin stabilization requires intact Wnt signaling. Supported by DOD W81XWH07-1-0562.
Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1000.
Abstract 1004: Investigating the role of Cdc42 in the developing mouse mammary gland
Authors: Bray Kristi M. , Vargo-Gogola Tracy 
Published: February 2011

Abstract
Rho GTPases coordinate and transmit extracellular signals to control downstream effectors. The Rho GTPase cell division cycle 42 (Cdc42) is overexpressed and hyperactivated in human breast cancers. Cdc42 regulates cell polarity, mitosis, adhesion, migration, and stem cell function. These same cellular processes also function in normal mammary gland development. Exactly which functions of Cdc42 are crucial for normal mammary gland (MG) development and its precise role in mammary tumor formation and progression remain unknown. We hypothesize that Cdc42 plays critical and pleiotropic roles in normal MG development and tumorigenesis through regulation of the signal transduction pathways that are involved in cell cycle progression, cell polarity determination, cell motility, and cell adhesion. To investigate the molecular mechanisms by which Cdc42 regulates MG development, we studied the effects of Cdc42 deletion on morphogenesis of primary mammary epithelial cells (MECs). Adeno-cre viral transduction was used to introduce cre recombinase into Cdc42fl/fl primary MECs, and effects on morphogenesis were assessed using a three-dimensional mammary morphogenesis assay. Transduction efficiency ranged from 50-80% and ∼70% percent knockdown of Cdc42 expression was achieved. Control Adeno-GFP transduced MECs developed into polarized mammary acini whereas morphogenesis was markedly perturbed in the Cdc42 deficient MECs. Cdc42 knockout MECs formed small (30 µm in diameter compared to 45 µm in the control structures, p &lt; .001, n = 28), disorganized structures lacking polarity and lumens (99% vs. 8% in the controls, n &gt; 30). Markers of normal development including polarity markers, pERM and α6; adhesion, ZO-1; and downstream signaling, pkc-ζ, displayed aberrant expression patterns in the knockout structures. Interestingly, Cdc42 deficient structures contained invasive cells, which were rarely seen in control acini (41% vs 7%, n=80), suggesting that loss of Cdc42 induces aberrant migration. Cdk4 levels were also reduced in Cdc42 deficient cells and cell cycle analysis by flow cytometry demonstrated an increase in the percentage of cells in G1, suggesting that loss of Cdc42 inhibits MEC proliferation by blocking the transition from G1 to S phase. In addition, analysis of an S phase marker, phospho-histone H3, was performed at an early time point (3 days) in morphogenesis. Only 35% of the transduced knockout structures expressed phospho-histone H3, compared to 100% in the controls (n&gt;20). These studies demonstrate a vital role for Cdc42 in the processes of growth, adherence, and polarity establishment during mammary gland development.
Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 1004.