bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro delivery, cytotoxicity, swelling, and degradation behavior of a liquid‐to‐solid gelling polymer system for cerebral aneurysm embolization
Authors: Brennecka Celeste R. , Preul Mark C. , Vernon Brent L. 
Published: April 2012

AbstractThis study examines the in vitro characteristics of a crosslinking polymer system for cerebral aneurysm embolization. The polymeric material is composed of poly(propylene glycol)diacrylate (PPODA) and pentaerythritol tetrakis(3‐mercaptopropionate) (QT), formulated with the liquid contrast agents Conray™ or Omnipaque™ 300. The PPODA–QT system was tested for delivery feasibility through mock delivery into a model aneurysm. Cytotoxicity was performed by exposing 3T3 cells to gel formulations, followed by a cell viability assay. Swelling was measured on samples submerged in 150 mM phosphate buffered saline at 37 or 50°C. The same samples underwent compression testing to assess degradation, characterized by reduction in Young's modulus over time. The PPODA–QT system was easily deliverable to mock aneurysms. Cytotoxicity results indicated that Conray‐formulated gels are initially less toxic than Omnipaque‐formulated gels, but show greater susceptibility to swelling and degradation over time. In general, these experiments represented more challenging conditions than would be present in vivo, and therefore, reported results are likely overestimations of in vivo outcomes. However, these results highlight potential issues with each PPODA–QT formulation. Given the desired outcome of neointimal tissue growth over the polymer material, initial cytotoxicity may be more important than long‐term factors when choosing an optimal formulation for aneurysm embolization. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 2012.
Expanding molecular modeling and design tools to non‐natural sidechains
Authors: Gfeller David , Michielin Olivier , Zoete Vincent 
Published: April 2012

AbstractProtein–protein interactions encode the wiring diagram of cellular signaling pathways and their deregulations underlie a variety of diseases, such as cancer. Inhibiting protein–protein interactions with peptide derivatives is a promising way to develop new biological and therapeutic tools. Here, we develop a general framework to computationally handle hundreds of non‐natural amino acid sidechains and predict the effect of inserting them into peptides or proteins. We first generate all structural files (pdb and mol2), as well as parameters and topologies for standard molecular mechanics software (CHARMM and Gromacs). Accurate predictions of rotamer probabilities are provided using a novel combined knowledge and physics based strategy. Non‐natural sidechains are useful to increase peptide ligand binding affinity. Our results obtained on non‐natural mutants of a BCL9 peptide targeting beta‐catenin show very good correlation between predicted and experimental binding free‐energies, indicating that such predictions can be used to design new inhibitors. Data generated in this work, as well as PyMOL and UCSF Chimera plug‐ins for user‐friendly visualization of non‐natural sidechains, are all available at http://www.swisssidechain.ch. Our results enable researchers to rapidly and efficiently work with hundreds of non‐natural sidechains. © 2012 Wiley Periodicals, Inc.
An unusual case of giant cardiac fibroelastoma mimicking left atrial myxoma in a patient presenting with syncope
Authors: Khoueiry Georges , Geha Fady , Meghani Mustafain , Abi Rafeh Nidal , Azab Basem , Torbey Estelle , Asgarian Kourosh T. , Sicat Michael 
Published: April 2012

AbstractCardiac papillary fibroelastomas are the most common primary valvular tumors. Generally benign, they account only for about 10% of all primary cardiac neoplasms, can occur in normal or diseased hearts, and are associated strongly with open heart surgery and radiotherapy. They are, in most cases, incidental findings, but can be discovered after syncope. We report the case of an elderly female, who was referred for syncope and was found to have a large fibroelastoma at the mitral valve annulus, intermittently obstructing the left ventricular inflow tract, and mimicking the presentation of left atrial myxoma. This case illustrates another potential mechanism of syncope in patients with fibroelastomas. © 2012 Wiley Periodicals, Inc. J Clin Ultrasound, 2013
In utero treatment of fetal goitrous hypothyroidism in a euthyroid mother: A case report
Authors: Stewart Chantal J.M. , Constantatos Sonia , Joolay Yaseen , Muller Linnie 
Published: April 2012

AbstractFetal goitrous hypothyroidism is a rare condition in euthyroid pregnant women. Complications such as tracheal and esophageal compression with resultant polyhydramnios, as well as the possibility of neurodevelopmental effects of hypothyroidism, have prompted prenatal treatment with intra‐amniotic L‐thyroxine. We report a case of this condition and its in utero management. © 2012 Wiley Periodicals, Inc. J Clin Ultrasound, 2012
Does lenticulostriate vasculopathy predipose to ischemic brain infarct? A case report
Authors: Ivanov Ivan , Zlatareva Dora , Pacheva Iliyana , Panova Margarita 
Published: April 2012

AbstractLenticulostriate vasculopathy (LSV) is a sonographic finding in infancy with obscure etiology and variable diagnostic and prognostic significance. Ischemic infarct in the territory of the lenticulostriate vessels after mild head trauma is a rare pathology. There are no publications on LSV followed by ischemic infarct. We present the case of an 8‐month‐old boy who suffered mild head trauma and developed an ischemic brain infarct in the territory of preexisting LSV. It is speculated that LSV might be a predisposing factor for ischemic brain infarct after mild head trauma in infants. © 2012 Wiley Periodicals, Inc. J Clin Ultrasound, 2012
Effect of low‐level laser therapy on intramembranous and endochondral autogenous bone grafts healing
Authors: Biguetti Claudia Cristina , Filho Emílio José Marquardt , de Andrade Holgado Leandro , Caviquioli Gustavo , Moreschi Eduardo , Comparin Eliston , Matsumoto Mariza Akemi 
Published: April 2012

AbstractThe aim of this study was to evaluate the healing process of intramembranous (IM) and endochondral (EC) bone grafts under low‐level laser therapy (LLLT). Male rabbits underwent onlay autogenous bone grafts (1 cm in diameter) retrieved from the calvaria and iliac crest and fixed on parietal bones, divided into four groups: Calvaria (C), Iliac (I), Calvaria + LLLT (C+L), and Iliac + LLLT (I+L). Animals from C+L and I+L Groups had their grafts exposed to LLLT (AlGaAs–808 nm, CW, 30 mW, 0.028 cm2 average laser beam area), 15 s irradiation time (16 J/cm2 per point–total of 64 J/cm2 per session). After 7, 14, 30, and 60 days, grafts were retrieved and resorption pattern analyzed by means of morphometry and TRAP‐positive osteoclasts detection. Differences in the resorption levels of iliac grafts were observed, presenting 40% in I group against 8% in I+L grafts at the 14th day of evaluation (P < 0.05). After 30 days, resorption was maintained at 41% in I group, whereas I+L presented 15% in the same period (P = 0.0591). No significant differences were noted in the rates of calvaria grafts resorption in all periods. A significant higher number of osteoclasts on the grafts' surface was observed in C+L Group at day 30, in comparison with C group. In I+L Group, prevalence of osteoclasts was marked at day 7 (P < 0.05) in comparison to I Group. In general, it was concluded that biomodulative effects of LLLT did not significantly affect healing and resorption processes of autogenous bone grafts from EC and IM origins. Microsc. Res. Tech. 75:1237–1244, 2012. © 2012 Wiley Periodicals, Inc.
Body mass, phylogeny and diet composition affects kidney morphology in passerine birds
Authors: Barceló Gonzalo , Salinas Jonathan , Sabat Pablo 
Published: April 2012

AbstractWe studied the renal morphology of 16 species of passerines to assess whether the composition of the diet of birds have been paralleled by differences in the characteristics of the kidneys. We determined the number and length of the medullary cones and the percentage of renal medulla in birds with contrasting dietary habits and then correlated these features with the percentage of nitrogen and proportion of invertebrates in the diet. To examine the correlation between kidney and diet variables, we first used standard correlation and least square regression, and correlation and regression on phylogenetically independent data. We found that the mass of the medullary portion of the kidney, and the medullary cone length were negatively correlated with the percentage of invertebrates present in the diet. We hypothesized that the further development of the renal medulla observed in granivorous birds may be correlated with the small amount of water present in the seeds. Our results suggest that the availability of water in different foods is probably one of the main factors that have led to structural and probably functional changes of the kidney in the studied species. J. Morphol., 2012. © 2012 Wiley Periodicals, Inc.
MicroRNA expression in mouse oligodendrocytes and regulation of proteolipid protein gene expression
Authors: Wang Erming , Cambi Franca 
Published: April 2012

AbstractOverexpression of the major myelin proteolipid protein (PLP) is detrimental to brain development and function and is the most common cause of Pelizaeus‐Merzbacher disease. microRNA (miRNA), small, noncoding RNAs, have been shown to play critical roles in oligodendrocyte lineage. In this study, we sought to investigate whether miRNAs control PLP abundance. To identify candidate miRNAs involved in this regulation, we have examined differentiation‐induced changes in the expression of miRNAs in the oligodendroglial cell line Oli‐neu and in enhanced green fluorescent protein positive oligodendrocytes ex vivo. We have identified 145 miRNAs that are expressed in oligodendrocyte cell lineage progression. Dicer1 expression decreases in differentiated oligodendrocytes, and knock down of Dicer1 results in changes in miRNAs similar to those associated with differentiation. To identify miRNAs that control the PLP expression, we have selected miRNAs whose expression is lower in differentiated vs. undifferentiated Oli‐neu cells and that have one or more binding site(s) in the PLP 3′‐untranslated region (3′UTR). The PLP 3′UTR fused to the luciferase gene reduces the activity of the reporter, suggesting that it negatively regulates message stability or translation. Such suppression is relieved by knock down of miR‐20a. Overexpression of miR‐20a decreases expression of the endogenous PLP in primary oligodendrocytes and of the reporter gene. Deletion or mutation of the putative binding site for miR‐20a in the PLP 3′UTR abrogated such effects. Our data indicate that miRNA expression is regulated by Dicer1 levels in differentiated oligodendrocytes and that miR‐20a, a component of the cluster that controls oligodendrocyte cell number, regulates PLP gene expression through its 3′UTR. © 2012 Wiley Periodicals, Inc.
Increase of ryanodine receptors by dopamine D1 receptors is negatively regulated by γ‐aminobutyric acid type B receptors in primary cultures of mouse cerebral cortical neurons
Authors: Kurokawa Kazuhiro , Mizuno Koji , Ohkuma Seitaro 
Published: April 2012

AbstractAlthough upregulation of ryanodine receptor (RyR)‐1 and ‐2 is mediated through the activation of dopamine D1 receptors (D1DRs) in the development of psychostimulant‐induced place preference, little is known about how such increased expressions of RyRs are negatively regulated. This study investigated negative regulatory mechanisms of increase of RyR‐1 and ‐2 expression by D1DR stimulation with its full agonist, SKF82958 or A 68930, using cultures of mouse cerebral cortical neurons. Sustained exposure to SKF82958 or A 68930 of the neurons increased RyR‐1 and ‐2 proteins in a dose‐ and time‐dependentmanner. The SKF82958‐induced increases of RyR‐1 and ‐2 proteins were significantly suppressed by SCH23390 (a selective D1DR antagonist). In addition, the SKF82958‐ or A 68930‐induced increases of RyR‐1 and ‐2 proteins were completely abolished by baclofen (a selective γ‐aminobutyric acid type B [GABAB] receptor agonist), whereas muscimol (an agonist specific to GABAA receptors) had no effect. SKF82958 or A 68930 significantly increased intracellular cAMP level, which was completely suppressed by baclofen. Furthermore, sustained exposure to phorbol 12,13‐dibutyrate, a protein kinase C activator, did not change the expression of RyR‐1 or ‐2 proteins. Immunohistochemical study showed colocalizaton of immunoreactivities for three types of proteins, D1DRs and GABAB receptor R1 and R2 subunits in the same neuronal bodies, suggesting that the neurochemical changes induced by the activation of D1DRs and GABAB receptors occur in the same neurons. These results indicate that RyR‐1 and ‐2 expression facilitated by D1DR stimulation are negatively regulated by GABAB receptor via suppression of cAMP production. © 2012 Wiley Periodicals, Inc.
Expression pattern of ataxia telangiectasia mutated (ATM), p53, Akt, and glycogen synthase kinase‐3β in the striatum of rats treated with 3‐nitropropionic acid
Authors: Duran‐Vilaregut Joaquim , Manich Gemma , del Valle Jaume , Camins Antoni , Pallàs Mercè , Vilaplana Jordi , Pelegrí Carme 
Published: April 2012

Abstract3‐Nitropropionic acid (3‐NPA) is a mitochondrial toxin used in the laboratory to replicate neurodegenerative conditions that are accompanied by degeneration of the caudate‐putamen. 3‐NPA induces depletion in ATP production, reactive oxygen species production, and secondary excitotoxicity mediated by activation of N‐methyl‐D‐aspartate receptors that culminates in the triggering of cell death mechanisms, including apoptosis. We here examined by immunohistochemical methods whether cellular expression of phosphoSer1981‐ataxia telangiectasia mutated (ATM), phosphoSer15‐p53, phosphoSer473‐Akt, and phosphoSer9‐glycogen synthase kinase‐3β (GSK3β), which are key signal molecules that play a critical role in regulating cellular processes related to cell survival and demise, were involved in the striatal neurodegeneration in the brains of rats treated with 3‐NPA. Our results indicate that the toxin induced the activation of ATM and p53 only in astrocytes, and a role for these proteins in neuronal degeneration was ruled out. On the other hand, striatal neurons lost the active form of Akt as soon as they began to appear pyknotic, indicating impairment of the PI3K/Akt/GSK3 pathway in their degenerative process. The inactive form of GSK3β was detected extensively, mainly in the rim of the striatal lesions around degenerating neurons, which could be attributed to a cell death or cell survival response. © 2012 Wiley Periodicals, Inc.
Thermoresponsive Poly(N‐isopropylacrylamide)‐Based Block Copolymer Coating for Optimizing Cell Sheet Fabrication
Authors: Nakayama Masamichi , Yamada Naoko , Kumashiro Yoshikazu , Kanazawa Hideko , Yamato Masayuki , Okano Teruo 
Published: April 2012

AbstractThermoresponsive surfaces are prepared via a spin‐coating method with a block copolymer consisting of poly(N‐isopropylacrylamide) (PIPAAm) and poly(butyl methacrylate) (PBMA) on polystyrene surfaces. The PBMA block suppresses the removal of deposited PIPAAm‐based polymers from the surface. The polymer coating affects the temperature‐dependent cellular behavior of the surfaces with respect to protein adsorption. By adjusting layer thicknesses, PBMA‐b‐PIPAAm‐coated surfaces are optimized to regulate the adhesion/detachment of cells by temperature changes. Thus, thermoresponsive polymer‐coated surfaces are able to harvest contiguous cell sheets with their basal extracellular matrix proteins.magnified image
Stochastic logistic equation with infinite delay
Authors: Liu Meng , Wang Ke 
Published: April 2012

This paper perturbs the famous logistic equation with infinite delay
urn:x-wiley:01704214:media:mma1608:mma1608-math-0001 into the corresponding stochastic system
urn:x-wiley:01704214:media:mma1608:mma1608-math-0002This study shows that the above stochastic system has a global positive solution with probability 1 and gives the asymptotic pathwise estimation of this solution. In addition, the superior limit of the average in time of the sample path of the solution is estimated. This work also establishes the sufficient conditions for extinction, nonpersistence in the mean, and weak persistence of the solution. The critical value between weak persistence and extinction is obtained. Then these results are extended to n‐dimensional stochastic Lotka–Volterra competitive system with infinite delay. Finally, this paper provides some numerical figures to illustrate the results. The results reveal that, firstly, different types of environmental noises have different effects on the persistence and extinction of the population system; secondly, the delay has no effect on the persistence and extinction of the stochastic system.Copyright © 2012 John Wiley & Sons, Ltd.
46‐W high efficiency unbalanced doherty power amplifier in extended output power back‐off
Authors: Jang Dong‐Hee , Kim Ji‐Yeon , Kim Jong‐Heon 
Published: April 2012

AbstractA 46‐W unbalanced Doherty amplifier is proposed to obtain high efficiency at the output power back‐off beyond 6 dB using the same type of transistors in both the main and the peaking amplifier.An added power efficiency of 38% and an adjacent channel power ratio of −35.6 dBc were measured for a CDMA2000 1FA signal. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:1612–1614, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26865
Design and experimental study of a fiber bragg grating chirped tuning method
Authors: Li Lijun , Ma Hui , Cao Hui , Ma Weihong , Yin Xingbin , Zhang Yong , Cao Maoyong 
Published: April 2012

AbstractIn this article, fiber Bragg grating chirped tuning method is theoretically and experimentally demonstrated. Using a double‐arched structure and a metal diaphragm, a vertical pressure can be transmitted to grating. According to different metal diaphragm shapes and grating pasted paths, by changing the pressure, fiber grating spectrum chirped tuning can be achieved. When it is pressurized, the internal strain distributions of metal diaphragm are simulated using finite element method. This uniform fiber Bragg grating chirped tuning method is also experimentally studied. The chirped tuning of fiber Bragg grating can be achieved. By introducing a correction factor, the theoretical and experimental results agree very well. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:1582–1585, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26866
Tri‐bandpass filter based on two stepped impedance resonators
Authors: Moussa Rachida , Palukuru Vamsi K. , Sowpati Arun K. , Essaaidi Mohamed , Jantunen Heli , Aghoutane Mohamed 
Published: April 2012

AbstractA novel approach for dual‐ and tri‐band bandpass filters (BPF) design with independently controlled center frequencies and bandwidths was presented. On the one hand, a stepped impedance resonator (SIR) is designed to operate at the center frequencies of two bands simultaneously (dual‐band), on the other hand, two SIR s are designed to operate at the center frequencies of three bands simultaneously (tri‐band). To verify the proposed approach, a tri‐BPF has been designed and fabricated with printed circuit board technology. The measured results are in good agreement with the simulation results. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:1765–1768, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26868
A meandered inverted‐f capsule antenna for an ingestible medical communication system
Authors: Seo Wonbum , Kim Uisheon , Lee Soonyong , Kwon Kyeol , Choi Jaehoon 
Published: April 2012

AbstractIn this article, a capsule antenna used in an ingestible medical device is proposed. To achieve miniaturization and a wide bandwidth, an inverted‐F antenna with a meandered strip line was used. The antenna performance in a human voxel model is analyzed through simulation, and the performance of the fabricated antenna is verified by comparing the measured data with that of the simulation, when the antenna is placed in a human‐equivalent liquid phantom. To investigate the performance of the proposed antenna in an indoor environment, a typical sickroom model is set‐up, and the propagation characteristics between the proposed antenna and the base station are analyzed at 1.4 GHz. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:1761–1765, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26869
A compact hepta‐band antenna for portable and embedded devices
Authors: Boldaji Ashkan , Bialkowski Marek E. 
Published: April 2012

AbstractThe article presents the design of a compact hepta‐band antenna for portable and embedded applications targeting the GSM‐900/DCS/PCS/UMTS‐2100 cellular and 2.4/5.2/5.5‐GHz WLAN bands. A printed inverted‐F antenna with a folded primary arm and a second radiating arm on the bottom surface of the substrate initially provides tri‐band operation in the 900, 1700, and 2450‐MHz bands. The shorting‐arm is relocated below the primary arm for a reduction in occupied area. The addition of a long slot in the ground plane offers an additional operation around 2100 MHz, bridging the 1700‐ and 2450‐MHz resonances and promoting significantly wide‐band performance of more than 900 MHz in that band. A second ground slot allows the antenna to support two of the 5‐GHz WLAN bands. Laboratory measurements confirm the performance of the final hepta‐band antenna, with 6‐dB return loss met from 880–1000, 1680–2590, and 5150–5710 MHz, and better than 10‐dB return loss at the centers and edges of these bands. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:1614–1618, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26872
A method to adjust enhanced X‐band microwave transmission and beaming using asymmetric gratings
Authors: Liu Yu , Cao Jin‐xiang , Liu Jun‐ming , Du Yin‐chang , Zheng Zhe , Wang Jian 
Published: April 2012

AbstractWe present a method to adjust enhanced X‐band microwave transmission and beaming through controlling the distance between grooves on the front and back surface of asymmetric gratings.Extraordinary transmission and beaming effect was influenced at X‐band microwave frequencies (8–12 GHz). It demonstrates a new way to achieve directional selectivity in microwave frequencies. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:1599–1601, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26874
Compact monopole antenna with two strips and a rectangular‐slit ground plane for dual‐band WLAN/WiMAX applications
Authors: Yoon Joong Han , Kil Gyung‐Suk 
Published: April 2012

AbstractA novel design of a simple microstrip‐fed monopole antenna for wireless local‐area network/worldwide interoperability of microwave access (WLAN/WiMAX) band operation is proposed in this article. The proposed antenna consists of two branch strip, an asymmetrical ground plane, and a rectangular‐slit ground plane. The proposed antenna is capable of generating two separate resonant modes with good impedance‐matching conditions. Prototypes of the proposed antenna were fabricated and experimentally measured. Acceptable agreement between the measurement and simulation results was achieved. The numerical and experiment results demonstrate that the proposed antenna satisfies the return loss −10 dB (VSWR < 1:2) impedance bandwidth requirement while simultaneously covering the WLAN and WiMAX bands. Meanwhile, the two‐dimensional radiation pattern and three‐dimensional gain performance of the antenna in the operation bands were also observed and discussed. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:1559–1566, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26875