bluuebunny/crossref_metadata_2025_split huggingface dataset:
Infection by Two Strains ofWolbachiaand Sex Ratio Distortion in a Population of the Endangered ButterflyZizina emelina(Lepidoptera: Lycaenidae) in Northern Osaka Prefecture, Central Japan
Authors: Sakamoto Yoshiko , Hirai Norio , Tanikawa Tetsuo , Yago Masaya , Ishii Minoru 
Published: May 2011

AbstractWolbachia infections were investigated by sequencing the wsp and ftsZ genes in a population of the endangered butterfly Zizina emelina (de l'Orza) (formerly Zizina otis emelina) (Lepidoptera: Lycaenidae), in Toyonaka City, northern Osaka Prefecture, central Japan. Wolbachia was detected in 65, 77, and 86% of field-collected adults in 2004, 2005, and 2007, respectively. Sequencing results revealed the presence of two strains of Wolbachia, wEmeTnl and wEmeTn2, in adults of this population, although no double infection was found. Three (12.5%) of 24 field-collected females infected by wEmeTnl produced offspring with a female-biased sex ratio, whereas all females infected by wEmeTn2 produced almost all-female offspring. In contrast, no uninfected females produced female-biased offspring. Moreover, the mean egg hatchability of broods from wEmeTn2-infected females was almost half that of uninfected females. These results indicate that at least wEmeTn2 is a male-killing strain.
Comparison of In Vivo Host Animals as Blood-Feeding Source for Laboratory Rearing of the Sandfly Vector Phlebotomus argentipes(Diptera: Psychodidae)
Authors: Kumar Vijay , Kesari Shreekant , Kumari Krishna , Krishnakumari B , Venugopalan R , Das Pradeep 
Published: May 2011

Abstract
Visceral leishmaniasis (VL) or kala-azar is transmitted by parasite-infected sandflies. The female sandfly Phlebotomus argentipes Annandale & Brunetti (Diptera: Psychodidae) carries the protozoan parasite Leishmania donovani (Kinetplastida: Trypanosomatidae) in its gut and injects the pathogen along with its saliva into the bloodstream of a human host. This article reports the successful setup of a very productive laboratory insectarium-based P. argentipes colony, by optimizing breeding conditions and choice of laboratory host animal for blood feeding of the female sandflies. The work also suggests that the source of blood feeding is a critical parameter for egg laying and maturation, which are essential for the maintenance of laboratory colonies of such hematophagous insects.
Life Table Analysis and Development of Singhiella simplex (Hemiptera: Aleyrodidae) Under Different Constant Temperatures
Authors: Legaspi Jesusa Crisostomo , Mannion Catharine , Amalin Divina , Legaspi Benjamin C 
Published: May 2011

Abstract
Singhiella simplex (Singh) (Hemiptera: Aleyrodidae) is a newly invasive pest of several species of Ficus plants in the United States. Very little is known about its biology and life history. Here, we studied its development and reproduction at 15, 20, 25, 27, 30, and 35°C. No immatures survived the 35°C treatment. Stage-specific duration times are presented for the other temperatures. Total duration of immature stages varied from 97.1 d at 15°C to 25.2 d at 30°C. Linear functions were used to describe development rates for eggs, instars and pupal stages. Total immature development also was modeled using a nonlinear Briere-1 function: r(T) = aT(T -T0) where r(T) is developmental rate at temperature (T), a = 0.0000146, T0 = 7.3120084 and TL = 45.9512202 (constant, lower developmental threshold, and lethal temperature, respectively). The thermal requirement for development from eggs to pupae was estimated to be 487.8 degree-days. S. simplex reproduction was highest at 27°C, where R0, GRR, T, r, λ, and DT were 23.114 ♀ / ♀, 24.25 ♀/♀, 31.413 d, 0.099 ♀ / ♀ /d, 1.105 ♀ / ♀ /d, and 6.93 d, respectively. The combined effect of temperature and female adult age on daily oviposition rate was modeled using the Enkegaard equation: eggmean = (p + qT) d exp(-wTd), where T is temperature. Parameter estimates were p = -30.21, q = 2.62, and w = 0.034. Duration of female adulthood was 8 d at 15°C, significantly longer than 2.5–4.2 d at the higher temperatures. At 25 and 27°C, lifetime fecundity per female averaged 37.9 and 46.2, respectively.
Synthetic 3,5-Dimethyldodecanoic Acid Serves as a General Attractant for Multiple Species of Prionus (Coleoptera: Cerambycidae)
Authors: Barbour James D , Millar Jocelyn G , Rodstein Joshua , Ray Ann M , Alston Diane G , Rejzek Martin , Dutcher James D , Hanks Lawrence M 
Published: May 2011

Abstract
Males of the longhorned beetle Prionus californicus Motschulsky (Coleoptera: Cerambycidae) are significantly attracted to the female-produced sex pheromone (3R,5S)-3,5-dimethyldodecanoic acid. Males respond equally well to the synthetic blend of the four stereoisomers of 3,5-dimethyldodecanoic acid as to the single natural enantiomer, suggesting that the unnatural isomers are not inhibitory. Males of the congener Prionus lecontei Lameere also are attracted to the (3R,5S)-enantiomer but not to the (3S,5R)-enantiomer, suggesting that the (3R,5S)-enantiomer is also an important pheromone component of that species. Here, we report the results of field trials that test the hypothesis that synthetic 3,5-dimethyldodecanoic acid will serve as a general attractant for males of other Prionus species. We conducted field bioassays of synthetic 3,5-dimethyldodecanoic acid at study sites in six different regions of North America and one site in the United Kingdom. Traps baited with the synthetic pheromone blend captured males of P.californicus (southwestern Idaho, southern California, and northwestern Utah), P. lecontei (southern California and northwestern Utah), and six additional species of Prionus: Prionus integer LeConte (southwestern Idaho), Prionus imbricornis (L.) (Georgia), Prionus laticollis (Drury) (Georgia), Prionus linsleyi Hovore (north central Arizona), Prionus aztecus Casey (northern Mexico), and Prionus coriarius (L.) (East Anglia, United Kingdom). These findings demonstrate that synthetic 3,5-dimethyldodecanoic acid can be used to assess the geographic distribution and local abundance of many Prionus species and therefore may be of value for monitoring threatened and endangered species of this genus, and for managing those that are pests.
Comment améliorer la gouvernance économique européenne ?
Authors: Saint-Etienne Christian 
Published: May 2011

How to improve the European economic governance
In the European Union, economic governance is not implemented through an elected body responsible in front of a Parliament, but it is conducted through three independent Institutions: the Stability and Growth Pact, the European Central Bank and the Competition Directory of the European Commission. The non coordination of these Institutions leads to the powerlessness of the economic government of Europe. Optimizing the European policy mix requires adjusting monetary and budgetary policies and exchange rates policies in order to reach the highest rate of growth without inflation and external deficit.
Modèles politico-économétriques et prévisions électorales pour mai 2007
Authors: Lafay Jean-Dominique , Facchini François , Auberger Antoine 
Published: May 2011

Politico-Econometric Models and Electoral Predictions for May 2007 in France
This paper discusses the question of electoral prediction based on politicoeconometric models. It presents a brief historical sketch of this specific research domain in political economy, and a synthesis of the present model-based predictions for the French 2007 presidential elections. Because the reviewed models predict different and somewhat opposed results, we suggest to use an ex ante arbitrage, based on a simple indicator, the Figaro-Sofres popularity index for the socialist party. The arbitrage between potential winners appears to be very clear. As this paper is written six weeks ante eventum, it can be seen as a kind of natural experiment in itself, useful to test the predictive capacity of our selected indicator.
La relation entre prix agricoles et prix alimentaires
Authors: Butault Jean-Pierre 
Published: May 2011

The Relationship Between Agricultural Prices and Food Prices
This paper examines the relationships between agriculture, food processing, trade and retail, and consumers at the macro-economic level in France, between 1978 and 2005. The surplus account method is applied using input-output tables (IOT). In order to do so, volume and price indices are calculated for all the elements of the IOTs' to measure the productivity of agriculture and food processing industries and measure the transfers of purchasing power between various economic agents. Agriculture includes forestry and fishing. Food processing industries are divided into two sub-sectors: meat and dairy, and other food processing industries. Trade and retail activities are measured by marketing margins.
To measure relationships between sectors, we use the table of intermediate inputs. Relationships with the rest of the world are taken into account through imports and exports. The subsidies and taxes are introduced either at the stage of production, or at the stage of use of products. Payments of factors in each sector are deducted from income accounts, their volume being calculated like an aggregate of the labour and fixed assets being used.
Incidence of Encapsulating Peritoneal Sclerosis: A Single-Center Experience with Long-Term Peritoneal Dialysis in the United States
Authors: Gayomali Charina , Hussein Usama , Cameron Scott F. , Protopapas Zenon , Finkelstein Fredric O. 
Published: May 2011

Encapsulating peritoneal sclerosis (EPS) is a serious complication of long-term peritoneal dialysis (PD). The reported incidence varies between 0.5% and 4.4% and increases with length of time on PD. Very few data are available on the epidemiology of EPS in the United States. The aim of the present study was assess the incidence of EPS in a single center in New Haven, Connecticut. In a retrospective analysis of all patients maintained on PD for 5 or more years, clinical symptoms were documented, abdominal computed tomography (CT) findings were reviewed, and surgical and pathology findings were noted. Patients were tracked whether they remained on PD, transferred to hemodialysis (HD), underwent transplantation, or died. Among the 76 patients that met the inclusion criteria (mean dialysis duration: 81.5 ± 22 months), 14 (18%) developed clinical symptoms (persisting for 3 or more months) suggestive of EPS. Abdominal CT imaging was done in 38 patients; 25 had radiologic features consistent with EPS. At laparotomy, 6 of 7 patients had gross findings consistent with EPS. Eleven patients met the 2000 criteria of the International Society for Peritoneal Dialysis for a diagnosis of EPS; they had clinical features, plus either radiologic or histopathologic confirmation. In 3 patients with clinical symptoms, the radiologic or surgical findings did not support a diagnosis of EPS. Of the 11 patients meeting the EPS criteria, 1 remains on PD and is doing well, 2 were transferred to HD and are doing well, 2 died as a result of EPS complications, and 6 died of other causes. The present study suggests that, in patients maintained on PD for 5 or more years at our center, the incidence of EPS is 14%. Those findings suggest that EPS may be under-recognized in the United States and that a high index of suspicion is warranted in patients maintained on PD for 5 or more years.
Inhibitory Effects of Indigowoad Root Polysaccharides on Porcine Reproductive and Respiratory Syndrome Virus Replication In Vitro
Authors: Wei Zhan-Yong , Wang Xue-Bing , Zhang Hong-Ying , Yang Chun-Hua , Wang Ya-Bin , Xu Duan-Hong , Chen Hong-Ying , Cui Bao-An 
Published: May 2011

Background Indigowoad root polysaccharide (IRPS) is a natural polysaccharide isolated from the traditional Chinese medicinal herb Radix Isatidis, and has many kinds of biological activities. However, the IRPS antiviral activity, especially the anti-porcine reproductive and respiratory syndrome virus (PRRSV) effect, has not been evaluated. Methods PRRSV was propagated in the MARC-145 cell line, and viral titre was determined by cytopathic effect and expressed as the 50% tissue culture infection dose (TCID50) in the current study. The cell cytotoxic effect of IRPS toward MARC-145 was evaluated by MTT assay firstly, then the inhibitory effects of IRPS on PRRSV replication in vitro were investigated by determining the effect of IRPS upon a single replicative cycle of PRRSV in MARC- 145 cells. The effects of IRPS on viral RNA and protein synthesis in PRRSV-infected cells were investigated using real-time PCR and double-antibody (sandwich) ELISA. Results IRPS was able to effectively suppress the infectivity of the PRRSV in a dose-dependent manner, especially by adding IRPS during the PRRSV infection. IRPS could affect the attachment of PRRSV to MARC-145 cells, and also inhibit the viral RNA and protein synthesis. Conclusions IRPS has an antiviral effect on PRRSV replication in MARC-145 cells and might be useful in medical development for antiviral research. However, the precise mechanism of the host and viral targets of IRPS are unknown, so further studies should be conducted to investigate the precise mechanism of IRPS inhibitory effect on PRRSV infection.
Preparing Nurse Educators to Use Simulation Technology: A Consortium Model for Practice and Education
Authors: Halstead Judith A. , Phillips Janet M. , Koller Angela , Hardin Karen , Porter Marjorie Lentz , Dwyer Jennifer S. 
Published: May 2011

Background:
As nurses seek to advance their education through online courses, considering best practices in feedback is especially important. Rich and rapid feedback has long been considered a best teaching practice, but how to provide this feedback in an online course environment is not always clear. This study was conducted to identify how experienced faculty provide feedback to online students.

Methods:
This descriptive exploratory study surveyed faculty about their approach to feedback with broad exploratory questions and a validation survey. The sample included faculty in four different states who benchmark online educational best practices.

Results:
Content analysis was used to evaluate data, with 15 themes emerging. These themes were organized into three categories: using best available tools; having a system; and creating a feedback-rich environment.

Conclusion:
The findings support and extend the guidelines for best practices in online education, including a focus on multisource feedback opportunities during course design.
Fixating Dislocated Intraocular Lens by 25-Gauge Vitrectomy
Authors: Kunikata Hiroshi , Fuse Nobuo , Abe Toshiaki 
Published: May 2011

BACKGROUND AND OBJECTIVES:
To describe a technique for suturing a dislocated intraocular lens (IOL) with 25-gauge (G) instruments.

PATIENTS AND METHODS:
Non-comparative interventional case series performed at a single center. Five patients with a dislocated IOL underwent 25-G transconjunctival sutureless vitrectomy (25G-TSV) with a temporary externalization of the haptics to fixate the IOL. Three IOLs enclosed in the lens capsule were dislocated into the vitreous and removed from the capsule with 25G-TSV using perfluorocarbon liquid. The best-corrected visual acuity and surgical complications resulting from this technique were recorded.

RESULTS:
In all cases, the IOL was fixed stably and remained well positioned. The visual acuity was improved or maintained in all cases. There were no intraoperative or postoperative complications except for one eye with a postoperative iris capture of the sutured IOL.

CONCLUSION:
Findings indicate that 25G-TSV is a practical and safe method for fixating a dislocated IOL.
Prevalence of Connective Tissue Diseases in Egyptian Patients Presenting with Fever of Unknown Origin
Authors: Abdelbaky Mohamad S. , Mansour Howaida E. , Ibrahim Shafika I. , Hassan Iman A. 
Published: May 2011

Objective To estimate the prevalence of connective tissue diseases in patients presenting with fever of unknown origin (FUO). Patients and Methods In this study thirty patients diagnosed as FUO (Group 1), in 2008, were included in an observational study and diagnostic workup. Additionally, retrospective analysis of seventy patients’ files (Group 2), for patients who presented with prolonged unexplained pyrexia to the same hospital in the previous two years, was performed. Patients were subjected to: full clinical assessment including full history taking, thorough clinical examination, laboratory investigations including the basic investigations for patients with prolonged fever, complete blood count, erythrocytes sedimentation rate, urine analysis and culture, blood culture, sputum culture and plain chest X ray. Further diagnostic work up and/or procedures were requested according to the potential diagnostic clues (PDC) present in every patient. Results Out of 100 FUO patients, 50% were found to have infectious diseases, 24% were found to have connective tissue diseases, 8% miscellaneous causes and 7% neoplastic diseases ( P < 0.05). In 11 patients no definite cause for FUO could be identified. Connective tissue patients were: eight systemic lupus patients (33.3%), five patients with familial mediterranean fever (20.8%), four patients with rheumatoid arthritis (16.6%), three patients (12.5%) with Still's disease and Rheumatic fever and one patient with Behçet syndrome/Crohn's disease (4.3%), ( P < 0.05). Conclusions Despite the advanced technology, FUO remains a challenging medical problem. Infections were the most common cause of FUO in Egypt, confirming the trends found in other parts of the world. There was an increased prevalence of connective tissue patients presented with prolonged unexplained fever. A keen clinical eye, meticulous history taking and repeated physical examination remained the most important diagnostic tools in FUO patients.
Tumor Cell Membrane‐Camouflaged Vortex Magnetic Nanoannulars Programmed by Low‐Frequency Magnetic Field: A Novel Anti‐Cancer Delivery System in Triple‐Negative Breast Cancer
Authors: Lu Yunshu , Gu Fenfen , Ma Yuwei , Da Xianhong , Li Ruonan , Jiang Lan , Li Xiang , Liu Yan 
Published: May 2024

AbstractTriple‐negative breast cancer (TNBC), a highly aggressive form of breast cancer, currently lacks targeted therapies and is associated with high recurrence rates in medical practice. In this study, the effectiveness of a specially developed magnetic nanodrug, namely doxorubicin (DOX)‐loaded Fe3O4 vortex magnetic nanoannulars coated with a cancer cell membrane (DOX‐VMAs@CM), against cancer under a varying low‐frequency magnetic field (MF), is investigated. This advanced nanodrug can specifically target and accumulate in tumors. Additionally, it considerably improves tumor suppression compared with that of DOX chemotherapy alone, indicating the possibility of homologous targeting of the cancer cell membrane and the vibrating effect of the MF. The DOX‐VMAs@CM nanoparticles (NPs) exhibit exceptional safety characteristics because of their ability to specifically target tumors. The results of RNA sequencing suggest that the potential mechanisms may involve cellular respiration, cell cycle, and ferroptosis regulation. Altogether, chemotherapy is successfully optimized by implementing a delivery system and MF intervention, and the developed DOX‐VMAs@CM NPs hold significant potential for use in the clinical treatment of TNBC.
Functional Integration of Synthetic Cells into 3D Microfluidic Devices for Artificial Organ‐On‐Chip Technologies
Authors: Hakami Niki , Burgstaller Anna , Gao Ning , Rutz Angela , Mann Stephen , Staufer Oskar 
Published: May 2024

AbstractMicrofluidics plays a pivotal role in organ‐on‐chip technologies and in the study of synthetic cells, especially in the development and analysis of artificial cell models. However, approaches that use synthetic cells as integral functional components for microfluidic systems to shape the microenvironment of natural living cells cultured on‐chip are not explored. Here, colloidosome‐based synthetic cells are integrated into 3D microfluidic devices, pioneering the concept of synthetic cell‐based microenvironments for organs‐on‐chip. Methods are devised to create dense and stable networks of silica colloidosomes, enveloped by supported lipid bilayers, within microfluidic channels. These networks promote receptor‐ligand interactions with on‐chip cultured cells. Furthermore, a technique is introduced for the controlled release of growth factors from the synthetic cells into the channels, using a calcium alginate‐based hydrogel formation within the colloidosomes. To demonstrate the potential of the technology, a modular plug‐and‐play lymph‐node‐on‐a‐chip prototype that guides the expansion of primary human T cells by stimulating receptor ligands on the T cells and modulating their cytokine environment is presented. This integration of synthetic cells into microfluidic systems offers a new direction for organ‐on‐chip technologies and suggests further avenues for exploration in potential therapeutic applications.
Development of Nanopillar Arrays Nanopatterning Without Lift‐Off for Transferable GaN‐Based µLEDs
Authors: Labchir Nabil , Hammami Saber , Baril Kilian , Wehbe Maya , Labau Sebastien , Reche Jerome , Petit‐Etienne Camille , Panabière Marie , Coulon Pierre‐Marie , Alloing Blandine , Munoz Daniel Pino , Zuniga‐Perez Jesus , Gergaud Patrice , Charles Matthew , Gourgon Cécile 
Published: May 2024

AbstractThe mass production of µLEDs requires an upscaling approach on 200 mm wafers, which implies the deployment of a technology that achieves zero defectivity without liftoff. In this report, Nanoimprint lithography (NIL) processing is successfully optimized for nanostructuring GaN‐based Silicon‐On‐Insulator (SOI) substrates. The etching of SiO2/GaN/AlN/Si/SiO2layers using different plasmas is conducted and multi‐layer nanopillars 100–200 mm in diameter are fabricated. This approach generates zero‐defect arrays of pillars, which is particularly advantageous for the growth process. In addition, the SiO2at the bottom of the pillar allows it to twist during the subsequent GaN regrowth, as this layer becomes soft at the growth temperature >1000 °C. This ability to deform enables a coalescence of pillars into layers with reduced dislocation density. As a result, high‐quality GaN microplatelets and µLEDs are grown via a bottom‐up approach based on pendeoepitaxy using metal–organic vapor phase epitaxy (MOVPE). The fabricated µLEDs have a very smooth surface with a roughness of 0.6 nm which facilitated the implementation of an easy and simple transfer protocol. Adhesive tape and metalmetal bonding, are used to bond the µLEDs onto a metal‐coated silicon substrate. The reported findings offer exciting new insights into the development of high‐performance displays.