bluuebunny/crossref_metadata_2025_split huggingface dataset:
Intradiscal temperature monitoring using double gradient‐echo pulse sequences at 1.0T
Authors: Wonneberger Uta , Schnackenburg Bernhard , Wlodarczyk Waldemar , Walter Thula , Streitparth Florian , Rump Jens , Teichgräber Ulf K.M. 
Published: May 2010

AbstractPurpose:To validate an unspoiled gradient‐recalled echo pulse sequence with dual echo acquisition as a means to increase temperature sensitivity while monitoring intradiscal laser ablation therapy.Materials and Methods:Phantom experiments as well as in vitro thermal ablation simulations were performed in an open 1.0T magnetic resonance (MR) scanner. Three methods of noninvasive MR‐thermometry based on the signal void decrease caused by T1‐relaxation time increase (T1), the temperature‐dependent proton resonance frequency (PRF) shift, and a combination of both methods with complex differences (CD) were compared. Temperature accuracy and reliability of temperature distribution were the main assessment criteria.Results:The optimum temperature sensitivity was found using CD in phantom experiments. During in vitro experiments the PRF showed the smallest margin of error (T1: ±1.64°C, PRF: ±1.23°C, CD: ±1.29°C) and the best qualitative evaluation of temperature.Conclusion:Intradiscal temperature monitoring with an unspoiled dual‐echo sequence is most accurate with PRF‐thermometry in combination with the long echo time. Magnitude images with an initial short echo time permit high image detail of the heat‐induced lesion. J. Magn. Reson. Imaging 2010;31:1499–1503. © 2010 Wiley‐Liss, Inc.
Correlation of cognitive dysfunction and diffusion tensor MRI measures in patients with mild and moderate multiple sclerosis
Authors: Hecke Wim Van , Nagels Guy , Leemans Alexander , Vandervliet Evert , Sijbers Jan , Parizel Paul M. 
Published: May 2010

AbstractPurposeTo compare the diffusion tensor imaging (DTI) measures of multiple sclerosis (MS) patients and healthy subjects in every brain voxel and to correlate them with Paced Auditory Serial Addition Test (PASAT) scores.Materials and MethodsFractional anisotropy (FA), and mean, longitudinal, and transverse diffusivity are compared between control subjects and MS patients, which were subdivided as mildly and moderately impaired. In addition, PASAT scores are correlated for both MS groups with the diffusion measures. An optimized voxel based analysis (VBA) method, in terms of coregistration, atlas construction, and image smoothing, was thereby used.Results:Diffusion differences between the control subjects and the patients with MS were found in the corpus callosum, inferior longitudinal fasciculus, cortico spinal tracts, forceps major, superior longitudinal fasciculus, and cingulum. In addition, we observed significant correlations of the FA and PASAT scores in the left inferior longitudinal fasciculus, the forceps minor, the capsula interna and externa, the genu of the corpus callosum, the left cingulum, the superior longitudinal fasciculus, and the corona radiata.Conclusion:Diffusion differences were observed between the mildly impaired MS patients and control subjects. In addition, different diffusion measures correlated with PASAT scores for cognitive decline in parietal, frontal, as well as temporal white matter (WM) regions. J. Magn. Reson. Imaging 2010;31:1492–1498. © 2010 Wiley‐Liss, Inc.
A method for measuring the cross sectional area of the anterior portion of the optic nerve in vivo using a fast 3D MRI sequence
Authors: Yiannakas Marios C. , Wheeler‐Kingshott Claudia A.M. , Berry Alaine M. , Chappell Karyn , Henderson Andrew , Kolappan Madhan , Miller David H. , Tozer Daniel J. 
Published: May 2010

AbstractPurpose:To investigate the three‐dimensional (3D) fast‐recovery fast spin‐echo accelerated (FRFSE‐XL) sequence as a new application for measuring the intraorbital optic nerve (ION) mean cross‐sectional area in vivo and to determine its value within a commonly used high resolution imaging protocol.Materials and Methods:The entire ION was scanned in nine healthy volunteers (mean age 32 ± 4 years) using the 3D FRFSE‐XL sequence and a commonly used high resolution short‐echo fast fluid‐attenuated inversion‐recovery (sTE fFLAIR) sequence with identical slice locations at 1.5T. The mean cross‐sectional area from both sequences was measured on a slice‐by‐slice basis from 3 mm behind the globe to the orbital apex. The reproducibility of both techniques was assessed from repeated scans (scan‐rescan) and repeated image analysis (intraobserver).Results:Measurement of the mean cross‐sectional area of the anterior 9 mm segment of the ION was only possible using the 3D FRFSE‐XL sequence with a mean area of 11.6 ± 2.2 mm2 (scan rescan COV = 3.3 ± 1.5, intraobserver COV = 2.4 ± 0.02) whereas the remainder segment of the ION (i.e., 9 mm behind the globe to the orbital apex) could only be measured with the use of the sTE fFLAIR with a mean area of 8.5 ± 1.7 mm2 (scan rescan COV = 4.9 ± 2.5 and intraobserver COV = 3.70 ± 0.03).Conclusion:The 3D FRFSE‐XL allows fast and reproducible measurement of the cross‐sectional area of the anterior 9mm segment of the ION, which is not possible using commonly used imaging sequences due to image degradation from motion, and is of complementary value to the existing imaging protocol for ION atrophy quantification. J. Magn. Reson. Imaging 2010;31:1486–1491. © 2010 Wiley‐Liss, Inc.
Prospective cytomegalovirus monitoring during first‐line chemotherapy in patients with acute myeloid leukemia
Authors: Capria Saveria , Gentile Giuseppe , Capobianchi Angela , Cardarelli Luisa , Gianfelici Valentina , Trisolini Silvia Maria , Foà Robin , Martino Pietro , Meloni Giovanni 
Published: May 2010

AbstractLittle is known about the incidence and clinical impact of cytomegalovirus (CMV) infection in patients with acute myeloid leukemia at the time of diagnosis and during chemotherapy. The aims of the present study were to assess prospectively the incidence of active CMV infection in 69 consecutive patients with acute myeloid leukemia and to describe the outcomes of treatment. pp65 antigenemia was monitored at diagnosis, post‐induction and post‐consolidation chemotherapy, and whenever CMV reactivation was suspected. Patients with pp65 antigenemia received pre‐emptive anti‐CMV treatment. Fifty‐nine patients achieved complete remission. Baseline CMV serology results were available for 56 of the 59 patients: 52 patients (93%) were IgG positive. The overall incidence of pp65 antigenemia in patients in complete remission after chemotherapy was 35% (21/59): 9 patients after induction and 12 post‐consolidation. Sixteen of the 21 pp65‐positive patients received anti‐CMV treatment: 15 as pre‐emptive therapy and 1 for interstitial CMV pneumonitis. Five patients received no anti‐CMV treatment and did not develop CMV disease. Patients with pp65 antigenemia had more hospital admissions (2.57 vs. 2.16; P = 0.009), while patients with >10 pp65‐positive cells had more clinical complications (8/9 vs. 2/12; P = 0.002). In conclusion, patients with acute myeloid leukemia receiving chemotherapy should be monitored for active CMV infection. CMV reactivation in these patients was associated with an increased number of hospital admissions, and high levels of pp65 antigenemia were associated with more clinical complications. Controlled studies are needed to assess the relevance of pre‐emptive anti‐CMV therapy in patients with acute myeloid leukemia receiving chemotherapy. J. Med. Virol. 82: 1201–1207, 2010. © 2010 Wiley‐Liss, Inc.
Human papillomavirus infection in urine samples from male renal transplant patients
Authors: Tornesello Maria Lina , Loquercio Giovanna , Tagliamonte Maria , Rossano Fabio , Buonaguro Luigi , Buonaguro Franco Maria 
Published: May 2010

AbstractRenal allograft recipients have a well‐documented increased incidence of human papillomavirus (HPV)‐related malignancies and preventive strategies should be specifically implemented. While in females the use of the Papanicolau test and HPV detection assay are used currently as a screening test for cervical cancer, no diagnostic procedures have been implemented to monitor HPV infection in males. The aim of this study was to test for HPV infection and to determine the spectrum of viral genotypes in urine samples of men with renal transplants. The study included 88 patients who underwent kidney transplantation between 1999 and 2005. HPV sequences were detected by nested PCR, using the broad‐spectrum consensus‐primer pairs MY09/MY11 and the new MGP system, and characterized by nucleotide sequence analysis. Overall, 43 (48.9%) samples were found positive for HPV sequences and the most common genotypes were HPV 16 (53.5%) and HPV 54 (9.3%) followed by HPV 6, 53, 56, 58, 66, 11, 12, 20, 45, 62, and 71, in descending order of prevalence. The majority of HPV 16 isolates were classified as European and only one as African‐1 variant on the basis of nucleotide signature present within the MGP L1 region. The high prevalence of HPV 16 among renal allograft recipients suggests that an HPV‐16‐based preventive or therapeutic vaccine may be effective for prevention or treatment of HPV‐related neoplasia in this group of immune compromised patients. J. Med. Virol. 82:1179–1185, 2010. © 2010 Wiley‐Liss, Inc.
Viral respiratory infections in hospitalized and community control children in Alaska
Authors: Singleton Rosalyn J. , Bulkow Lisa R. , Miernyk Karen , DeByle Carolynn , Pruitt Lori , Hummel Kimberlee Boyd , Bruden Dana , Englund Janet A. , Anderson Larry J. , Lucher Lynne , Holman Robert C. , Hennessy Thomas W. 
Published: May 2010

AbstractRespiratory syncytial virus (RSV) in Alaska Native children from the Yukon Kuskokwim (YK) Delta is associated with a hospitalization rate five times higher than that reported for the general US child population. The role of other viral respiratory pathogens has not been studied in this population. YK Delta children <3 years of age hospitalized with respiratory infections and same aged community control children were prospectively enrolled between October 2005 and September 2007. Polymerase chain reaction detection of viruses was performed on nasopharyngeal samples. Characteristics of hospitalized and asymptomatic control children were analyzed. From October 2005 to September 2007, 440 hospitalized and 425 control children were analyzed. Respiratory viruses were detected in 90% (395) of hospitalized children: 194 (44%) rhinovirus, 131 (30%) adenovirus, 102 (23%) RSV, 77 (18%) para influenza viruses (PIV), 66 (15%) human metapneumovirus (hMPV), 23 (5%) influenza, and 25 (6%) coronavirus. Fifty‐two percent (221) of control children had a virus detected, most commonly rhinovirus (33%), and adenovirus (16%). RSV, PIV, hMPV, and influenza were significantly more common in hospitalized cases than control children, but rhinovirus, adenovirus, and coronavirus were not. RSV and hMPV were associated with higher severity of illness. In this study, RSV remains the most important virus associated with respiratory hospitalization, although hMPV and PIV were also common. RSV and hMPV were associated with more severe illness. Rhinovirus and adenovirus were detected in two‐thirds of hospitalized children, but their frequent detection in control children made their role in respiratory hospitalization uncertain. J. Med. Virol. 82:1282–1290, 2010. © 2010 Wiley‐Liss, Inc.
Correlation between rotavirus A genotypes detected in hospitalized children and sewage samples in 2006, Córdoba, Argentina
Authors: Barril P.A. , Giordano M.O. , Isa M.B. , Masachessi G. , Ferreyra L.J. , Castello A.A. , Glikmann G. , Nates S.V. 
Published: May 2010

AbstractRoutine rotavirus A (RV‐A) surveillance is based on clinical cases, so only symptomatic infections are reported. The objective of this study was to determine whether the RV‐A genotypes and cold seasonal pattern described in patients with diarrhea is reflected by sewage surveillance, which could be representative of the RV‐A genotypes circulating in the population. The genotype distribution of RV‐A in effluent samples from a local sewage treatment plant was compared to those from local clinical cases. A total of 52 sewage samples and 70 stool specimens from children with acute non‐bacterial diarrhea were collected from January to December 2006. The effluent specimens were concentrated and RNA extracts from concentrated sewage and clinical samples were genotyped for the rotavirus VP7 gene. The proportional distribution of the RV‐A G‐genotypes in sewage and clinical samples during the cold season was similar: G1 accounted for 26.6% of the typed sewage isolates and 28.8% of the clinical infections; G3 type accounted for 21.9% and 25.8%; G2 type 15.6% and 10.6%; G4 type 17.2% and 21.2%; G8 type 1.6% and 0%; and the G9 type 17.2% and 13.6%, respectively. A similar picture of RV‐A genotype detection was obtained in sewage samples collected during the cold and warm seasons. The results indicate that there is a correlation between genotypes of RV‐A isolates from human diarrheic patients and of those from sewage samples. In addition, sewage monitoring highlighted the uniform all‐year RV‐A circulation, which was in contrast to the peak incidence of RV‐A infection in the community. J. Med. Virol. 82: 1277–1281, 2010. © 2010 Wiley‐Liss, Inc.
Application of PCR‐LDR‐nucleic acid detection strip in detection of YMDD mutation in hepatitis B patients treated with lamivudine
Authors: Xu Gaolian , You Qimin , Pickerill Sam , Zhong Huayan , Wang Hongying , Shi Jian , Luo Ying , You Paul , Kong Huimin , Lu Fengmin , Hu Lin 
Published: May 2010

AbstractChronic hepatitis B virus (CHBV) infection causes cirrhosis and hepatocellular carcinoma. Lamivudine (LAM) has been successfully used to treat CHBV infections but prolonged use leads to the emergence of drug‐resistant variants. This is primarily linked to a mutation in the tyrosine–methionine–aspartate–aspartate (YMDD) motif of the HBV polymerase gene at position 204. Rapid diagnosis of drug‐resistant HBV is necessary for a prompt treatment response. Common diagnostic methods such as sequencing and restriction fragment length polymorphism (RFLP) analysis lack sensitivity and require significant processing. The aim of this study was to demonstrate the usefulness of a novel diagnostic method that combines polymerase chain reaction (PCR), ligase detection reaction (LDR) and a nucleic acid detection strip (NADS) in detecting site‐specific mutations related to HBV LAM resistance. We compared this method (PLNA) to direct sequencing and RFLP analysis in 50 clinical samples from HBV infected patients. There was 90% concordance between all three results. PLNA detected more samples containing mutant variants than both sequencing and RFLP analysis and was more sensitive in detecting mixed variant populations. Plasmid standards indicated that the sensitivity of PLNA is at or below 3,000 copies per ml and that it can detect a minor variant at 5% of the total viral population. This warrants its further development and suggests that the PLNA method could be a useful tool in detecting LAM resistance. J. Med. Virol. 82: 1143–1149, 2010. © 2010 Wiley‐Liss, Inc.
Molecular and clinical characteristics of hepatitis B virus in Korea
Authors: Ahn Sang Hoon , Yuen Lilly , Han Kwang‐Hyub , Littlejohn Margaret , Chang Hye Young , Damerow Hans , Ayres Anna , Heo Jeong , Locarnini Stephen , Revill Peter A. 
Published: May 2010

AbstractKorea is an endemic area of hepatitis B virus (HBV) infection but very little is known about the molecular characteristics of HBV isolates from Korean patients or the association with disease progression. The complete HBV genome sequences from 53 Korean patients with chronic hepatitis B, advanced cirrhosis, or hepatocellular carcinoma (HCC) were analyzed to identify (i) subgenotype distribution and genetic diversity and (ii) signature mutations associated with liver disease progression. With the exception of 1 patient infected with HBV/B, all 52 patients (98.1%) were infected with HBV/C, subgenotype C2. These strains were 98.4% identical and the frequency of amino acid substitutions occurring within key immunological epitopes increased with disease severity. A number of amino acid/nucleotide substitutions were associated with HCC, namely sR24K (HBsAg), SI126T (HBsAg), and pcA1846T (precore gene) mutations (P = 0.029, 0.001, and 0.008, respectively). HBV harboring deletions in the pre‐S region were also associated with increased liver disease severity (chronic hepatitis B vs. cirrhosis, P = 0.040; chronic hepatitis B vs. HCC, P = 0.040). Despite the high degree of sequence conservation, several key HBV mutations were associated with disease progression. Prospective studies with larger cohorts of patients are required to evaluate further the clinical manifestation of HBV/C2 in Korea. J. Med. Virol. 82: 1126–1134, 2010. © 2010 Wiley‐Liss, Inc.
Surgical technique refinements in head and neck oncologic surgery
Authors: Liu Jeffrey C. , Shah Jatin P. 
Published: May 2010

AbstractThe head and neck region poses a challenging arena for oncologic surgery. Diseases and their treatment can affect a myriad of functions, including sight, hearing, taste, smell, breathing, speaking, swallowing, facial expression, and appearance. This review discusses several areas where refinements in surgical techniques have led to improved patient outcomes. This includes surgical incisions, neck lymphadenectomy, transoral laser microsurgery, minimally invasive thyroid surgery, and the use of vascularized free flaps for oromandibular reconstruction. J. Surg. Oncol. 2010; 101:661‐668. © 2010 Wiley‐Liss, Inc.
Regional treatment of colorectal liver metastasis
Authors: Kulaylat Mahmoud N. , Gibbs John F. 
Published: May 2010

AbstractCompared to systemic chemotherapy, intraarterial administration of tumoricidal agents with or without systemic chemotherapy in the treatment of nonresectable metastatic colorectal cancer is associated with superior response rate. The time‐to‐hepatic progression is also increased but the effect on overall survival is variable. When combined with other treatment modalities the number of patients who benefit from the treatment increases. In a subgroup of patients with resectable disease, hepatic artery infusion of chemotherapy as adjuvant therapy may be beneficial. Progression of the disease, hepatic and systemic toxicity, and complexity of the surgical aspects of the treatment limit the routine use of this treatment modality. J. Surg. Oncol. 2010; 101:693‐698. © 2010 Wiley‐Liss, Inc.
Refinements of surgical technique in soft tissue sarcomas
Authors: Karakousis Constantine P. 
Published: May 2010

AbstractThe primary modality of treatment for a localized soft tissue sarcoma is surgical resection. Adjuvant or neoadjuvant radiation helps reduce the rate of local recurrence. The rate of limb preserving resection is 94% in our series. Local recurrence can be re‐excised in 95% of the patients, with limb preservation in 90%. Retroperitoneal sarcomas present difficulties in resection but with optimal techniques the complete resectability rate approaches 95% with 5‐ and 10‐year survivals rates of 65% and 56%. J. Surg. Oncol. 2010; 101:730‐738. © 2010 Wiley‐Liss, Inc.
Advances in the surgical treatment of esophageal cancer
Authors: Ng Thomas , Vezeridis Michael P. 
Published: May 2010

AbstractSurgical resection remains the predominant modality in the management of esophageal cancer. Transthoracic and transhiatal esophagectomy are the procedures that are most frequently performed. Minimally invasive esophagectomy is feasible but will require further evaluation with well‐designed trials and long‐term follow‐up before it can be widely adopted. Technical improvements have lowered the rate of cervical anastomotic leak and improved the management of thoracic anastomotic leak. Outcome studies demonstrated that the optimal mortality, morbidity, and survival outcomes are obtained when esophageal resections are performed by experienced surgeons in high‐volume institutions. J. Surg. Oncol. 2010; 101:725‐729. © 2010 Wiley‐Liss, Inc.
Salvage of pelvic recurrence of colorectal cancer
Authors: Varker Kimberly A. , Wanebo Harold J. 
Published: May 2010

AbstractAlthough the incidence of locally recurrent colorectal cancer has been reduced by improved surgical techniques and the frequent use of multimodality therapy, pelvic recurrence remains a significant problem. Radiation or chemotherapy may provide palliation but it is often short‐lived. For fit candidates without evidence of extrapelvic disease, surgical resection (anterior resection, abdominoperineal resection, pelvic exenteration, or abdominosacral resection) may be the most appropriate treatment. For patients with unresectable disease, isolated pelvic perfusion may provide effective palliation. J. Surg. Oncol. 2010; 101:649‐660. © 2010 Wiley‐Liss, Inc.
Ein Lasersystem, viele Anwendungen
Authors: Schilling Niels , klotzbach Udo , Hendow Sami T. , Guerreiro Paulo T. 
Published: May 2010

AbstractDie fortschreitende Entwicklung der Faserlaser ermöglicht auf Grund der brillanten Strahlqualität dieser Systeme immer neue Anwendungsmöglichkeiten in der industriellen Fertigung. Gleichzeitig werden etablierte Prozesse von Festkörper‐ und Gaslasern aufgrund des guten Wirkungsgrades auf die Anwendung des Faserlasers ausgelegt. Trotz vieler wirtschaftlicher Vorteile dieses Lasersystems gegenüber etablierten Lasersystemen sind die Anschaffungskosten bei Kaufüberlegungen vor allem für kleine und mittelständische Unternehmen ausschlaggebend. Wichtige Kennzeichen zum Amortisieren des Systems sind weitreichende Einsatzspektren und die Möglichkeit der spezifischen Prozessoptimierung.
Mit Hybridanlagen anforderungsgerecht produzieren
Authors: Ernst Max 
Published: May 2010

AbstractImmer individuellere Aufträge erfordern immer flexiblere Produktionsprozesse. Mit Standardanlagen lässt sich das nur mit erhöhtem Kostenaufwand realisieren. Dagegen ermöglichen individuell gefertigte Maschinen, die beispielsweise das Laserschweißen und ‐schneiden in einem integrieren, das passgenaue Optimieren der Prozessabläufe und eine ungleich höhere Prozesssicherheit – und dies bei einem zumeist geringeren Kostenaufwand.
Resetting the Laser Market
Authors: Hausken Tom 
Published: May 2010

AbstractThe last year was one of the worst declines for the laser market in many years, in an industry used to steady growth. Reset to about the same level as 2003–2004, the drop destroyed profits, jobs, and forced some companies to close. If you survived 2009, you are already better off than many.On the positive side, it's all up from here. Our forecast shows growth in every segment from this new low point; a downturn with an upward‐sloping bottom. This downturn was sudden and companies reacted quickly. Revenues hit bottom in early 2009, already turning around by mid‐2009. Current indications are that output continues to grow in 2010.
Current awareness in prenatal diagnosis
Authors:   John Wiley & Sons, Ltd.
Published: May 2010

AbstractIn order to keep subscribers up‐to‐date with the latest developments in their field, John Wiley & Sons are providing a current awareness service in each issue of the journal. The bibliography contains newly published material in the field of prenatal diagnosis. Each bibliography is divided into 22 sections: 1 Reviews; 2 General Interest; 3 Normal Fetal Development; 4 Preimplantation Genetic Diagnosis; 5 First Trimester Diagnosis; 6 Second Trimester Diagnosis; 7 Fetal Imaging: General; Ultrasound; MRI; Doppler; 8 Maternal Serum Screening for Aneuploidy; 9 Screening for Carriers of Genetic Abnormality; 10 Molecular Cytogenetics: Metaphase Cytogenetics/FISH; Array CGH; 11 Fetal Cells in Maternal Circulation; 12 Fetal DNA/RNA in Maternal Body Fluids; 13 Fetal Therapy; 14 Psychosocial and Ethical Aspects; 15 Epidemiology and Environmental Factors; 16 Developmental and Placental Pathology; 17 Genetic Counseling. Within each section, articles are listed in alphabetical order with respect to author. If, in the preceding period, no publications are located relevant to any one of these headings, that section will be omitted