bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of acute myeloid leukaemia associated microRNA expression patterns
Authors: Isken Fabienne , Steffen Björn , Merk Sylvia , Dugas Martin , Markus Birgit , Tidow Nicola , Zühlsdorf Michael , Illmer Thomas , Thiede Christian , Berdel Wolfgang E. , Serve Hubert , Müller‐Tidow Carsten 
Published: June 2010

SummaryMicroRNAs (miRNAs) play an important role in cellular differentiation and cancer pathogenesis. This study analysed the expression of 154 human miRNAs in acute myeloid leukaemia (AML) and control samples using a stem‐loop real‐time reverse transcription polymerase chain reaction approach. Global patterns of miRNA expression in AML, normal bone marrow (NBM) and CD34+ progenitor cells allowed correct class predictions similar to whole genome microarray expression analyses that were performed at the same time. At single miRNA species level, MIRN23B was repressed in AML specimens compared to NBM and purified CD34+ haematopoietic progenitor cells. In contrast, the MIRN221/MIRN222 cluster and MIRN34A were expressed at significantly higher levels in AML blasts. Patients with high MIRN221/MIRN222 expression showed low levels of KIT RNA and protein expression but the correlation between kit protein and KIT mRNA was significantly stronger than the correlation of either one with MIRN221/MIRN222. A global analysis between miRNA expression levels and mRNA expression of predicted target genes revealed only weak associations in the majority of miRNA species. Nonetheless, the presence of two or more miRNA binding sites within the mRNA was usually associated with a decrease in mRNA levels. Taken together, these findings provide evidence that specific miRNA expression patterns exist in AML.
Parathyroid hormone stimulates the endothelial expression of vascular endothelial growth factor
Authors: Rashid G. , Bernheim J. , Green J. , Benchetrit S. 
Published: June 2010

ABSTRACTBackground  We showed previously that parathyroid hormone (PTH) may stimulate the endothelial expression of pro‐atherosclerotic and pro‐inflammatory markers. Considering the impact of PTH on vasculature, we decided to evaluate its effect on mRNA and intra‐cellular protein expressions of endothelial vascular endothelial growth factor (VEGF) taking into account that VEGF may play a role in the pathogenesis of endothelial dysfunctions.Materials and methods  Human umbilical vein cords endothelial cells (HUVEC) were stimulated for 24 h with 10−12–10−10 mol L−1 PTH. The VEGF‐165 mRNA expression (critical in stimulating endothelial cell proliferation) was evaluated by RT/PCR and the intra‐cellular VEGF protein expression by flow cytometry. The pathways by which PTH may have an effect on VEGF expression were also evaluated.Results  PTH (10−10 mol L−1) significantly increased VEGF‐165 mRNA expression (P < 0·05). The addition of 50 nmol L−1 protein kinase C (PKC) and 10 µmol L−1 protein kinase A (PKA) inhibitors significantly reduced the VEGF‐165 mRNA expression (P = 0·01). We also examined whether nitric oxide (NO) may be involved in the PTH‐induced stimulation of VEGF‐165 expression. Pre‐treatment of the cells with 200 µmol L‐nitro arginine methyl ester (L‐NAME, NO synthase inhibitor) was found to inhibit VEGF‐165 mRNA expression (P = 0·006). VEGF protein could not be detected in the medium of HUVEC but it was present in the cell cytoplasm. PTH had no significant effect on cytoplasmatic VEGF protein expression.Conclusion  The stimulatory effect of PTH on endothelial VEGF‐165 mRNA expression is partly through PKC and PKA pathways and is also NO dependent.
Treatment responses to cladribine and dasatinib in rapidly progressing aggressive mastocytosis
Authors: Aichberger K. J. , Sperr W. R. , Gleixner K. V. , Kretschmer A. , Valent P. 
Published: June 2010

ABSTRACTBackground  Systemic mastocytosis (SM) is a mast cell neoplasm in which neoplastic cells usually display the D816V‐mutated variant of KIT. Cladribine (2CdA) and dasatinib are two drugs that counteract the in vitro growth of neoplastic mast cells in SM. However, only little is known about the in vivo effects of these drugs in SM.Patient and methods  We report on a patient with highly aggressive interferon‐alpha‐resistant SM who was treated with 2CdA and dasatinib. In vitro pretesting revealed a response of neoplastic mast cells to both compounds with reasonable IC50 values.Results  The patient was treated with six cycles of 2CdA (0·13 mg kg−1 intravenously daily on 5 consecutive days). Despite a short‐lived major clinical response and a decrease in serum tryptase, the patient progressed to mast cell leukaemia after the sixth cycle of 2CdA. The patient then received two further courses of 2CdA followed by treatment with dasatinib (100 mg per os daily). However, no major response was obtained and the patient died from disease progression after 2 months.Conclusions  In a patient with rapidly progressing aggressive SM, neither 2CdA nor dasatinib produced a long‐lasting response in vivo, despite encouraging in vitro results. For such patients, alternative treatment strategies have to be developed.
Role of glucocorticoid receptor polymorphism in adrenal incidentalomas
Authors: Morelli Valentina , Donadio Francesca , Eller‐Vainicher Cristina , Cirello Valentina , Olgiati Luca , Savoca Chiara , Cairoli Elisa , Salcuni Antonio Stefano , Beck‐Peccoz Paolo , Chiodini Iacopo 
Published: June 2010

Eur J Clin Invest 2010; 40 (9): 803–811AbstractBackground  Adrenal incidentalomas (AI) have been associated with and an increased prevalence of metabolic and bone complications. The N363S and BclI polymorphisms of the glucocorticoid receptor (GR) have been associated with an increased sensitivity to glucocorticoid (GC). This observational study aims to evaluate whether BclI and N363S polymorphisms play a role in the development of complications in AI.Materials and methods  We enrolled 100 patients with AI (66 F; 34M). The presence of diabetes, arterial hypertension (AH), dyslipidaemia, osteoporosis and vertebral fracture (Fx), waist circumference and the Body Mass Index (BMI) were assessed. DNA samples were genotyped. Patients with wild‐type BclI, wild‐type N363S and heterozygous BclI polymorphism were classified as carriers of haplotype 1 (H1; n = 86), patients with homozygous BclI and heterozygous N363S polymorphism of GR of haplotype 2 (H2; n = 14).Results  We found no clinical or biochemical differences between haplotype 1 and 2 groups, but a higher prevalence of the simultaneous presence of Fx plus AH in H2 patients (H2 n = 7, H1 n = 16, P = 0·01). Logistic regression analysis showed that the presence of Fx and of AH and the combination of the presence of Fx plus AH were associated with the H2 genotype regardless of the degree of cortisol secretion, age, BMI and BMD (OR 4·88, 95%CI 1·47–18·40, P = 0·05; OR 8·25, 95%CI 0·98–69·52, P = 0·05; OR 7·25, 95%CI 1·57–35·78, P = 0·011; respectively).Conclusions  In AI patients, the presence of the haplotype 2 of BclI and N363S is associated with the presence of AH, Fx and with the combination of Fx and AH.
Secondary vascular changes in pulmonary sequestrations
Authors: Desai Saral , Dusmet Michael , Ladas George , Pomplun Sabine , Padley Simon P G , Griffin Nyree , Badreddine Jamal , Goldstraw Peter , Nicholson Andrew G 
Published: June 2010

Desai S, Dusmet M, Ladas G, Pomplun S, Padley S P G, Griffin N, Badreddine J, Goldstraw P & Nicholson A G (2010) Histopathology 57, 121–127 Secondary vascular changes in pulmonary sequestrationsAims:  Whilst parenchymal changes in pulmonary sequestrations are well described, there are comparatively little data on associated vascular changes and their extent. The aim of this study was to retrospectively review morphological changes within sequestrations, concentrating on vascular changes and associations with clinical parameters.Methods and results:  Twenty‐seven resected cases of sequestrations (intralobar n = 20, extralobar n = 7) showed a male predominance (n = 16) and an age range of 2 months–60 years (average 13 years). Plexogenic vascular changes (medial hypertrophy and intimal fibrosis) were seen in 15 of 27 cases, as well as plexiform lesions in seven cases. Patients with plexogenic changes had a higher mean age compared with those lacking vascular changes (19 versus 6 years) and were more commonly female. Respiratory tract infections were associated solely with intralobar sequestrations. No other associations between presenting symptoms and histopathological parameters were identified. Adjacent lung showed lesser plexogenic changes in six of 22 intralobar cases. There were features of type 2 congenital cystic adenomatoid lesions in 63% of cases. Dissection of the supplying systemic artery (n = 1), intralesional aspergilloma (n = 1) and coexistent lymphangiomatosis (n = 1) were also identified.Conclusions:  Hypertensive vascular changes are not uncommon in both intrapulmonary and extrapulmonary sequestrations, although their relative severity seems unrelated to presenting symptoms.
Expression of the reversion‐inducing cysteine‐rich protein with Kazal motifs and matrix metalloproteinase‐14 in neuroblastoma and the role in tumour metastasis
Authors: Dong Qian , Yu Dan , Yang Chuan‐Min , Jiang Buxian , Zhang Hong 
Published: June 2010

SummaryNeuroblastoma is the most common malignant tumour in infancy; the reversion‐inducing cysteine‐rich protein with Kazal motifs gene (RECK) is a tumour suppressor gene. Previous studies show that RECK inhibits tumour invasion and metastasis through negative regulation of the matrix metalloproteinase (MMP)‐2, MMP‐9 and MMP‐14. Therefore, we wanted to detect the expression of RECK and MMP‐14 in neuroblastomas to assess the correlation between the expression levels of these proteins, and to investigate the roles in the metastasis and development of the tumour. PV‐6000 immunohistochemistry method was used to detect the expression levels of RECK and MMP‐14 in 36 samples of neuroblastoma tissue. Samples from paraffin wax‐embedded specimens and the complete clinicopathological data of 36 neuroblastoma and 10 ganglioneuroma patients were collected. The rate of expression of the RECK protein in the neuroblastoma was low (16.7%). Furthermore, it reduced with the increase in the invasive depth and distant metastasis (P = 0.015; P < 0.05). The rate of expression of the MMP‐14 protein in the neuroblastoma was high (58.3%) and increased with the increase in the extent of invasive depth and distant metastasis (P = 0.002; P < 0.05). The expression of the RECK protein correlated negatively with that of MMP‐14 (r = −0.418; P < 0.05). Low levels of the RECK protein are expressed in the neuroblastoma, while the MMP‐14 protein is expressed at high levels. The RECK and MMP‐14 proteins may serve as markers in the estimation of the extent of metastasis and dissemination of the neuroblastoma.
Occupational allergy to bell pepper pollen in greenhouses in the Netherlands, an 8‐year follow‐up study
Authors: Patiwael J. A. , Jong N. W. , Burdorf A. , Groot H. , Gerth van Wijk R. 
Published: June 2010

To cite this article: Patiwael JA, Jong NW, Burdorf A, Groot H, Gerth van Wijk R. Occupational allergy to bell pepper pollen in greenhouses in the Netherlands, an 8‐year follow‐up study. Allergy 2010; 65: 1423–1429.AbstractBackground:  Pollen from bell pepper plants cultivated in greenhouses are known to cause occupational allergic disease. A cross‐sectional study among 472 bell pepper employees in 1999 estimated prevalences for sensitization to bell pepper pollen of 28% and for work‐related allergic symptoms of 54%. There is scarce information on the incidence of work‐related allergy and its risk factors. Aims of the present study were to estimate the cumulative incidence of work‐related symptoms and sensitization to bell pepper pollen, and to determine risk factors for the onset of these symptoms and sensitization.Methods:  Bell pepper employees who participated in a cross‐sectional survey in 1999 were asked to take part in a follow‐up study in 2007. Information on demographic characteristics, job characteristics and allergic symptoms was gathered by means of a questionnaire. Furthermore, skin tests were performed with a.o. bell pepper pollen.Results:  In total, 280 of 472 employees were available for questionnaires and in 250 employees allergy tests were performed. During the 8‐year follow‐up, the cumulative incidence of sensitization to bell pepper pollen was 9% and of work‐related rhinitis 19%. Atopy [odds ratio (OR) 5.60] and smoking (OR 3.53) were significantly associated with development of rhinitis. The cumulative incidence of work‐related asthma symptoms was 8%. Again atopy (OR 5.03) and smoking (OR 11.85) were significant risk factors.Conclusion:  Cumulative incidences for sensitization to bell pepper pollen, work‐related rhinitis and asthma symptoms were 9%, 19% and 8%, respectively. Atopy and smoking are risk factors for developing work‐related symptoms among workers in bell pepper horticulture.
Glucocorticoids for the treatment of anaphylaxis: Cochrane systematic review
Authors: Choo K. J. L. , Simons E. , Sheikh A. 
Published: June 2010

To cite this article: Choo KJL, Simons E, Sheikh A. Glucocorticoids for the treatment of anaphylaxis: Cochrane systematic review. Allergy 2010; 65: 1205–1211.AbstractBackground:  Anaphylaxis is a serious hypersensitivity reaction that is rapid in onset and may result in death. A number of guidelines recommend glucocorticoids for the treatment of people experiencing anaphylaxis.Objectives:  We sought to assess the benefits and harms of glucocorticoid treatment during episodes of anaphylaxis.Methods:  We searched the Cochrane Central Register of Controlled Trials (CENTRAL) (The Cochrane Library 2009, Issue 3), MEDLINE (Ovid) (1966 to September 2009), EMBASE (Ovid) (1988 to September 2009), CINAHL (EBSCOhost) (to September 2009) and The Science Citation Index Expanded (SCI‐EXPANDED) (1945 to September 2009). We also searched the UK National Research Register and websites listing ongoing trials and contacted international experts in anaphylaxis in an attempt to locate unpublished material. We sought to include randomized and quasi‐randomized controlled trials comparing glucocorticoids with any control (either placebo, adrenaline (epinephrine), an antihistamine, or any combination of these). Two authors independently assessed articles for inclusion.Results:  None of the 2496 reports identified satisfied the inclusion criteria.Conclusions:  We conclude that there is no evidence from high‐quality studies for the use of steroids in the emergency management of anaphylaxis. Therefore, we can neither support nor refute the use of these drugs for this purpose.
Early markers for protective mechanisms during rush venom immunotherapy
Authors: Bussmann C. , Xia J. , Allam J.‐P. , Maintz L. , Bieber T. , Novak N. 
Published: June 2010

To cite this article: Bussmann C, Xia J, Allam J‐P, Maintz L, Bieber T, Novak N. Early markers for protective mechanisms during rush venom immunotherapy. Allergy 2010; 65: 1558–1565.AbstractBackground:  Allergen‐specific venom immunotherapy (VIT) represents the only rational‐based option to treat allergic sensitizations against bee and wasp venom. So far, there is not much knowledge about early induction of protective and tolerogenic pathways during VIT.Objectives:  To identify the earliest markers for protective mechanisms against allergic reactions in the peripheral blood during the build‐up phase of VIT.Methods:  PBMC and monocytes were isolated, and serum samples were taken before and during a five day build‐up phase from 65 hymenoptera venom allergic patients. Expression level of tolerogenic markers was analyzed on mRNA and protein level. Serum levels of different soluble tolerogenic factors were measured.Results:  We observed significantly enhanced tryptophan degradation, elevated ILT4 expression of monocytes as well as IL‐10 production of CD3+ T cells only a few hours after the first injection on day 1, followed by increased IL‐10 serum levels, monocyte apoptosis and elevated intracellular cAMP levels of monocytes on day 3 combined with a higher ILT3 protein expression and IL‐10 secretion of monocytes on day 5.Conclusion:  From these data, we conclude that tryptophan depletion, ILT3/4‐mediated inhibition, higher IL‐10 production as well as intracellular cAMP might contribute to early induction of protective mechanisms against allergic reactions during the build‐up phase of VIT.
Root System Size Influences Water‐Nutrient Uptake and Nitrate Leaching Potential in Wheat
Authors: Ehdaie B. , Merhaut D. J. , Ahmadian S. , Hoops A. C. , Khuong T. , Layne A. P. , Waines J. G. 
Published: June 2010

AbstractEnvironmental and economic considerations require the effective use of water and nutrients to elevate grain production in bread wheat (Triticum aestivumL.) with concomitant reduction in nitrate leaching to minimize contamination of underground water. We determined the effect of the root system on leaching fraction, leachate N concentration, and N, P and K uptake using bread wheat ‘Pavon 76’ and its three near‐isogenic translocation lines: Pavon 1RS.1AL, Pavon 1RS.1BL and Pavon 1RS.1DL. These genotypes were grown in sand‐tube experiments under optimum and low level of nutrients for 2 years. Root, stem and leaves, and grain N, P, and K content, and agronomic characters were measured. Leaching fraction and leachate nitrate concentration were measured at early tillering, booting and early grain filling. Significant main effects for year, nutrient level and genotype were found for the characters. Genotype × N interaction was significant only for root P content. Genotype × year interaction was significant only for plant N content, root P content and plant P content. Genotype × year × N interaction was significant only for root N uptake efficiency. Thus, genotypic means averaged across years and nutrient levels are reported. Low levels of nutrients (1330, 235 and 793 mg vs. 1915, 375 and 1268 mg N, P and K, respectively) reduced mean root biomass, plant biomass and grain yield by 27 %, 25 %, and 19 %, respectively. The translocation lines produced 31–46 % more root biomass, 11–14 % heavier grains and 6–8 % greater grain yield than Pavon 76. Leaching fraction was higher under low level of nutrient at booting and grain filling. Leaching fraction at tillering, booting, and grain filling was 67%, 42% and 25%, respectively. Leaching fraction at early tillering was lower for Pavon 1RS.1AL (39 %) and Pavon 1RS.1DL (40.5 %) than for Pavon 76 (45.3 %). Leachate nitrate concentration was lower for two translocation lines at all three stages of plant growth compared to Pavon 76. The correlation coefficient between plant N content and root biomass, between plant N content and plant biomass, and between grain yield and root biomass was positive and significant. Significant positive correlation was found between root biomass and P and K uptake. Multiple small applications of N fertilizer during early plant growth with adequate irrigation water are recommended. Wheat genotypes with superior root characteristics for efficient nutrient uptake, especially during tillering and booting, should be developed in breeding programmes to increase grain yield and to minimize the nitrate leaching.
Dendrochronological assessment of the time since death of dead wood in an old growth Magellan's beech forest, Navarino Island (Chile)
Authors: LOMBARDI FABIO , COCOZZA CLAUDIA , LASSERRE BRUNO , TOGNETTI ROBERTO , MARCHETTI MARCO 
Published: June 2010

AbstractThe present study investigated the relationship between time since death and the morphological characteristics of fallen dead trees in a Nothofagus betuloides forest stand located on the island of Navarino (Chile). In this unmanaged forest, there were 399 m3 ha−1 of dead wood, which represented about half of the living tree volume. At the investigation site, 18 living trees were selected and increment cores were collected from them to build master ring‐width chronologies. Cross sections were also collected from 48 fallen dead trees. The samples collected were then assigned to observable decay classes and their death date was determined dendrochronologically. Cross‐dating techniques were used and it was found that the fallen dead trees cross‐dated significantly with standard chronologies. A year of death was successfully determined for 75% of the sampled fallen dead trees. However, this study demonstrated that, in the standard classification, the transition rate from one class of decay to another was highly variable. Furthermore, the inconsistencies found in the decay rates of the fallen dead trees demonstrated that the existing decay classification schemes were unsuitable for this type of forest stand and that the relationship between qualitatively assessed decay classes and the time since death of trees in this extreme environment was rather weak. In addition, the analysis of the time since death, in this old growth forest, was indicative of the persistence of dead wood on the forest floor in austral cold ecosystems and of its contribution to long‐term carbon storage.
Local‐scale spatial dynamics of ants in a temperate agroecosystem
Authors: CHONG CHEE‐SENG , HOFFMANN ARY A. , THOMSON LINDA J. 
Published: June 2010

AbstractAt the local scale, spatial aggregations in ant distribution are often thought to be driven by competitive interactions among dominant ant species, although niche preferences and habitat heterogeneity might also lead to patchiness. Nevertheless, competitive interactions might be particularly important in agroecosystems that are structurally more homogeneous than natural habitats. The spatial patterns of ants in two Australian vineyards were investigated by intensive pitfall trapping to examine if non‐random patterns occur and whether these might be the result of competitive species interactions as well as the influence of woody vegetation adjacent to the vineyards. Null model analyses suggested competitive species interactions within ant assemblages that might have been driven by numerically dominant species, even though both positive and negative associations between these were found. Consistent spatial aggregations indicated significant spatial overlap in distributions of some species. Such overlap suggests that potential coexistence might be attributed to temporal partitioning or differences in foraging strategies. The presence of woody vegetation had a marked influence on ant assemblage structure and competitive interactions, and might facilitate coexistence by increasing resource heterogeneity. The implications of these findings for sampling strategies and ecological processes within vineyards are discussed.