bluuebunny/crossref_metadata_2025_split huggingface dataset:
Dynamical Responses Predict a Distal Site that Modulates Activity in an Antibiotic Resistance Enzyme
Authors: Beer Michael , Oliveira Ana Sofia F. , Tooke Catherine L. , Hinchliffe Philip , Li Angie Tsz Yan , Balega Balazs , Spencer James , Mulholland Adrian J. 
Published: April 2024

Abstractβ-Lactamases, which hydrolyse β-lactam antibiotics, are key determinants of antibiotic resistance. Predicting the sites and effects of distal mutations in enzymes is challenging. For β-lactamases, the ability to make such predictions would contribute to understanding activity against, and development of, antibiotics and inhibitors to combat resistance. Here, using dynamical non-equilibrium molecular dynamics (D-NEMD) simulations combined with experiments, we demonstrate that intramolecular communication networks differ in three class A SulpHydryl Variant (SHV)-type β-lactamases). Differences in network architecture and correlated motions link to catalytic efficiency and β-lactam substrate spectrum. Further, the simulations identify a distal residue 89 in the clinically importantKlebsiella pneumoniaecarbapenemase 2 (KPC-2), as a participant in similar networks, suggesting that mutation at this position would modulate enzyme activity. Experimental kinetics, biophysical and structural characterisation of the naturally occurring, but previously biochemically uncharacterised, KPC-2G89Dmutant with several antibiotics and inhibitors reveals significant changes in hydrolytic spectrum, specifically reducing activity towards carbapenems without effecting major structural or stability changes. These results show that D-NEMD simulations can predict distal sites where mutation affects enzyme activity. This approach could have broad application in understanding enzyme evolution, and in engineering of natural andde novoenzymes.
Investigating the translational value of Periprosthetic Joint Infection (PJI) models to determine the risk and severity of Staphylococcal biofilms
Authors: Sekar Amita , Fan Yingfang , Tierney Peyton , McCanne Madeline , Jones Parker , Malick Fawaz , Kannambadi Devika , Wannomae Keith K , Inverardi Nicoletta , Muratoglu Orhun , Oral Ebru 
Published: April 2024

AbstractWith the advent of antibiotic-eluting polymeric materials for targeting recalcitrant infections, using preclinical models to study biofilm is crucial for improving the treatment efficacy in periprosthetic joint infections. The stratification of risk and severity of infections is needed to develop an effective clinical dosing framework with better outcomes. Here, using in-vivo and in-vitro implant-associated infection models, we demonstrate that methicillin-sensitive and resistantStaphylococcus aureus(MSSA and MRSA) have model-dependent distinct implant and peri-implant tissue colonization patterns. The maturity of biofilms and the location (implant vs tissue) were found to influence the antibiotic susceptibility evolution profiles of MSSA and MRSA and the models could capture the differing host-microbe interactions in vivo. Gene expression studies revealed the molecular heterogeneity of colonizing bacterial populations. The comparison and stratification of the risk and severity of infection across different preclinical models provided in this study can guide clinical dosing to effectively prevent or treat PJI.
A catalogue of verified and characterized arterial enhancers for key arterial identity genes
Authors: Nornes Svanhild , Bruche Susann , Adak Niharika , McCracken Ian , Val Sarah De 
Published: April 2024

ABSTRACTThe establishment and growth of the arterial endothelium requires the coordinated expression of numerous genes. However, the transcriptional and signalling pathways regulating this process are still not fully established, and only a small number of enhancers for key arterial genes have been characterized. Here, we sought to generate a useful and accessible cohort of arterial enhancers with which to study arterial transcriptional regulation. We combinedin silicoanalysis with transgenic zebrafish and mouse models to find and validate enhancers associated with eight key arterial identity genes (Acvrl1/Alk1,Cxcr4, Cxcl12, Efnb2, Gja4/Cx37, Gja5/Cx40,Nrp1andUnc5b). This identified a cohort of enhancers able to independently direct robust transcription to arterial ECs within the vasculature. To elucidate the regulatory pathways upstream of arterial gene transcription, we determined the occurrence of common endothelial transcription factor binding motifs, and assessed direct binding of these factors across all arterial enhancers compared to similar assessments of non-arterial-specific enhancers. These results find that binding of SOXF and ETS factors is a shared event across arterial enhancers, but also commonly occurs at pan-endothelial enhancers. Conversely, RBPJ/Notch, MEF2 and FOX binding was over-represented but not ubiquitous at arterial enhancers. We found no shared or arterial-specific signature for canonical WNT-associated TCF/LEF transcription factors, canonical TGFβ/BMP-associated SMAD1/5 and SMAD2, laminar shear stress-associated KLF factors or venous-enriched NR2F2 factors. This cohort of well characterized and in vivo-verified enhancers can now provide a platform for future studies into the interaction of different transcriptional and signalling pathways with arterial gene expression.
Right-handed slepton bulk regions for dark matter in a generalized minimal supergravity
Authors: Khan Imtiaz , Ahmed Waqas , Li Tianjun , Raza Shabbar 
Published: April 2024

We study the light right-handed slepton bulk regions for dark matter from the generalized minimal supergravity (GmSUGRA) in the minimal supersymmetric Standard Model. In our comprehensive numerical studies, we show that Rϕ˜≳10% is a conservative criterion to formulate bulk region, where Rϕ˜≡(mϕ˜−mχ˜10)/mχ˜10. For right-handed stau as the next-to-the-lightest supersymmetric particle (NLSP), we find a large viable parameter space, consistent with the current LHC constraints, Planck2018 dark matter relic density bounds, and direct bounds on neutralino-nucleons scattering cross section that naturally supports the right-handed stau bulk regions for dark matter. In particular, the upper bounds on the masses of the lightest supersymmetric particle neutralino and right-handed stau are about 120.4 and 138 GeV, respectively. This bulk region may be beyond the current LHC reach and could be probed at LUX-ZEPLIN, a next-generation dark matter direct-detection experiment, the Future Circular Collider at CERN, and the Circular Electron Positron Collider. However, the scenario with the right-handed selectron as the NLSP is excluded by the LHC supersymmetry searches.

Published by the American Physical Society
2024
Solving the left-hand cut problem in lattice QCD:
Tcc(3875)+
from finite volume energy levels
Authors: Meng Lu , Baru Vadim , Epelbaum Evgeny , Filin Arseniy A. , Gasparyan Ashot M. 
Published: April 2024

We discuss a novel effective-field-theory-based approach for extracting two-body scattering information from finite volume energies, serving as an alternative to Lüscher’s method. By explicitly incorporating one-pion exchange, we overcome the challenging left-hand cut problem in Lüscher’s method and can handle finite volume energy levels both below and above the left-hand cut. Applied to the lattice data for DD* scattering at a pion mass of 280 MeV, as an illustrative example, our results reveal the significant impact of the one-pion exchange on P-wave and S-wave phase shifts. The pole position of the Tcc(3875)+ state, extracted from the finite-volume energy levels at this pion mass while taking into account left-hand cut effects, range corrections and partial-wave mixing, is consistent with a near-threshold resonance. This study demonstrates, for the first time, that two-body scattering information can be reliably extracted from lattice spectra including the left-hand cut.

Published by the American Physical Society
2024
A double-loop adaptive relevant vector machine combined with Harris Hawks optimization-based importance sampling
Authors: Fan Xin , Liu Yongshou , Gu Zongyi , Yao Qin 
Published: April 2024

PurposeEnsuring the safety of structures is important. However, when a structure possesses both an implicit performance function and an extremely small failure probability, traditional methods struggle to conduct a reliability analysis. Therefore, this paper proposes a reliability analysis method aimed at enhancing the efficiency of rare event analysis, using the widely recognized Relevant Vector Machine (RVM).Design/methodology/approachDrawing from the principles of importance sampling (IS), this paper employs Harris Hawks Optimization (HHO) to ascertain the optimal design point. This approach not only guarantees precision but also facilitates the RVM in approximating the limit state surface. When the U learning function, designed for Kriging, is applied to RVM, it results in sample clustering in the design of experiment (DoE). Therefore, this paper proposes a FU learning function, which is more suitable for RVM.FindingsThree numerical examples and two engineering problem demonstrate the effectiveness of the proposed method.Originality/valueBy employing the HHO algorithm, this paper innovatively applies RVM in IS reliability analysis, proposing a novel method termed RVM-HIS. The RVM-HIS demonstrates exceptional computational efficiency, making it eminently suitable for rare events reliability analysis with implicit performance function. Moreover, the computational efficiency of RVM-HIS has been significantly enhanced through the improvement of the U learning function.
How performance measurement systems enable or hinder organizational ambidexterity
Authors: Badu Daniella Abena , Micheli Pietro 
Published: April 2024

PurposeThis study aims to examine how different uses of performance measurement systems (PMS) enable or hinder organizational ambidexterity (OA), intended as the simultaneous pursuit of exploitation and exploration.Design/methodology/approachFollowing a qualitative research design, we gathered data through semi-structured interviews, observations and reviews of documents at four departments of an automotive firm.FindingsWe contribute to operations management research and practice by demonstrating how PMS, which are typically associated with exploitation, can also foster exploration and enable organizations to become ambidextrous. Specifically, we show how PMS can be structured and used in more agile ways and, in relation to innovation, we identify which PM practices should be introduced and with what effects and those that should be avoided. We also contribute to organization theory by highlighting how a single management tool can promote the achievement of both exploration and exploitation.Practical implicationsIn investigating PMS uses and their effects, we identify several positive practices. For example, we show how managers can use PMS more effectively and how targets could be deployed to stimulate creativity and innovation. We also emphasize the need for managers to opt more often for team incentives rather than individual ones to encourage the collaboration needed for OA.Originality/valueWe provide in-depth insight into how PM tools affect an organization’s ability to pursue exploitation and exploration, thus contributing to research in operations, innovation and organization theory.
Vocational teachers’ perceptions on workplace learning in facilitating students’ professional engagement in the context of industry-university collaboration in China
Authors: Zhao Yanmin , Ko James 
Published: April 2024

Purpose
This study aims to explore vocational teachers' perceptions regarding workplace learning that align with students' training models and collaborative teaching involving specialised professionals within the context of industry-university collaboration.

Design/methodology/approach
Using a qualitative approach, the study conducted nine semi-structured interviews from three subject areas to better understand how vocational teachers’ work-based learning enhances their pedagogical practice in guiding students’ professional training. Thematic analysis was adopted to identify patterns that emerged from concepts and theories related to coding categories.

Findings
The authors identified three key components: vocational teachers’ workplace learning in connection with students’ training models, collaborative teaching with specialised professionals and teachers’ regular interactions with enterprises. The findings demonstrate that vocational teachers’ engagement in workplace learning pertaining to specific subjects provides a valuable avenue for enhancing curriculum design with collaboration with industry experts. This is key for supporting vocational students’ transitions into the workplace and ensuring their knowledge and skills are tailored to the industry-standard practice.

Research limitations/implications
The data are limited to the review of interviews from three vocational subject areas as the representative sector in the study. However, this research implies effective knowledge transfer between workplace settings and vocational institutions, and vocational teachers need to integrate work-based vocational knowledge and skills in a relevant and applicable way across diverse classroom settings.

Practical implications
Fostering collaborative partnerships with local industries and professionals can be a primary way to facilitate authentic learning experiences that are linked to a specific vocational field and bridge the gap between diverse classroom learning and real-world work scenarios.

Originality/value
This study combines contemporary workplace learning theories with the conceptual understanding of vocational teachers’ involvement with industry-specific practice. Connecting teachers’ knowledge to the industry extends the input and collaboration from professionals and field experts to the diverse vocational classrooms.
Open educational resources (OER) management: implications for policymakers on the best practices in an open education university libraries in Nigeria
Authors: Ailakhu Ugonna Vivian , Ibrahim Mohammed Gadaffi 
Published: April 2024

PurposeThe study aimed to identify the best practices for open educational resources (OER) management in Nigerian open education university libraries and the implications for policymakers.Design/methodology/approachA mixed-methods approach was used, which included a survey questionnaire administered via Google Forms to academic librarians and other library staff of the National Open University of Nigeria (NOUN). The study’s population was 398 and 273 participants responded to the survey. The study included all academic librarians and other library staff representing the six geopolitical zones in Nigeria. The data were analyzed using both qualitative and quantitative methods. The study’s results were presented using frequency counts, percentages tables and the qualitative data analysis was done thematically.FindingsThe findings of the study are consistent with existing literature on best practices for managing OER, which emphasized the importance of establishing clear policies and strategies for OER management practices, such as guidelines for acquiring, creating, curating and disseminating OER.Originality/valueThe originality of the study concludes by recommending that policymakers should develop policies that support the implementation of best practices in OER management to improve access to educational resources and reduce costs for students.
Investigating mental health and well-being among MBA students during campus placement season in India
Authors: Laeequddin Mohammed , Abdul Waheed Kareem , Sahay Vinita 
Published: April 2024

Purpose
This paper aims to identify the factors that influence students' mental health, particularly in the context of MBA students passing through an emotional phase of the placement season.

Design/methodology/approach
A conceptual model through literature has been proposed. To test the proposed model of this study, a survey was conducted among the students of three MBA institutes of national reputation in India. The study's hypotheses were investigated using partial least squares-structural equations modeling and analysis of variance. To corroborate the findings of the survey data, a qualitative study in the form of open-ended interviews with five students was conducted.

Findings
The study revealed that female students, non-engineering graduates and students from non-family business backgrounds undergo stress, anxiety and depression higher than their classmates. Cumulative grade point average and bank loans do not significantly affect students' stress, anxiety and depression during the placement season. It was found that the increase in the levels of mindfulness scores led to a significant negative impact on stress, anxiety and depression among the students.

Originality/value
There is a gap in the literature that addresses the mental health of MBA students during campus job placements and the role of mindfulness in mitigating stress, anxiety and depression in these students. This research attempts to fill these research gaps.
Material inheritance transmission by older childless Portuguese adults
Authors: Silva Daniela , Patrão Marta , Sousa Liliana 
Published: April 2024

Purpose
Material inheritance transmission is a normative task in later life that has been scarcely studied, particularly when involving older childless adults. This study aims to deepen the knowledge about the transmission of material inheritances by older (65+ years) childless Portuguese adults.

Design/methodology/approach
This qualitative exploratory study comprises 43 participants. A semi-structured interview was administered and submitted to content analysis.

Findings
Material inheritance transmission involves precipitant events (realizing they will not have children), deciding on content and moment (what and when), planning (getting legal advice, making a will and preventing family conflicts), distributing (usually based on values of reciprocity and strategic exchange) and resolution (marked by conflict).

Originality/value
Material inheritances have been mostly studied from economic and legal perspectives. This research examines material inheritances transmissions within family dynamics, showing that older childless individuals seem to use material inheritances in a strategic exchange way, to ensure they will be well cared for in old age.
Curcumol reduces lower limb arteriosclerosis in rats by inhibiting human arterial smooth muscle cell activity
Authors: Yang Shengzhuang , Huang Gaosheng , Liang Xiangsen , Sun Yu , Xian Lei 
Published: April 2024

AbstractCardiovascular diseases, particularly those involving arterial stenosis and smooth muscle cell proliferation, pose significant health risks. This study aimed to investigate the therapeutic potential of curcumol in inhibiting platelet‐derived growth factor‐BB (PDGF‐BB)‐induced human aortic smooth muscle cell (HASMC) proliferation, migration and autophagy. Using cell viability assays, 5‐ethynyl‐2′‐deoxyuridine (EdU) incorporation assays and Western Blot analyses, we observed that curcumol effectively attenuated PDGF‐BB‐induced HASMC proliferation and migration in a concentration‐dependent manner. Furthermore, curcumol mitigated PDGF‐BB‐induced autophagy, as evidenced by the downregulation of LC3‐II/LC3‐I ratio and upregulation of P62. In vivo experiments using an arteriosclerosis obliterans model demonstrated that curcumol treatment significantly ameliorated arterial morphology and reduced stenosis. Additionally, curcumol inhibited the activity of the KLF5/COX2 axis, a key pathway in vascular diseases. These findings suggest that curcumol has the potential to serve as a multi‐target therapeutic agent for vascular diseases.
Dyslexia: ‘The right diagnosis … The wrong treatment’
Authors: Beckett Jonathan 
Published: April 2024

AbstractThe polarisation of attitudes towards dyslexia appears to be contingent upon the awareness of the implications of adopting a medical model to interpreting dyslexia (as a medical ‘condition’) or applying an educational overlay, based upon difficulties in certain areas, to interpreting and making provision for those with dyslexia. Specifically, it underscores the difficulties dyslexics have in reading and the comprehension of what has been read. Furthermore, it accentuates the approaches taken in the 1980s and the turn of the century to dyslexia was, for these interviewees, unfitting. However, it raises other questions about whether the current practice is suited to the needs of those with dyslexia, or whether a universal offer is made, regardless of specific need. In the wake of this research, three emergent themes occur, dyslexia and stigmatisation, the impact of stigmatisation and poorly suited provision upon self‐esteem and the stress this generates. As key practitioner points, this disquisition underlines the need to tailor provision for inclusive education based upon the needs of the individual with dyslexia. It further illumines the stress of unsuitable provision, manifesting the imperative nature of adequate training and resourcing for dyslexia.
An integrated CRITIC‐EDAS approach for assessing enterprise crisis management effectiveness based on Weibo
Authors: Liu Shuhan , Chen Shuai , Wu Peng , Wu Qun , Zhou Ligang , Deveci Muhammet , Mardani Abbas 
Published: April 2024

AbstractIn the digital age, social media (such as Weibo, Twitter), as a rich source of data, has become a vital platform for businesses to communicate and engage with their audiences. In this paper, we investigate crisis management effectiveness for enterprises using social media. To achieve this goal, a CRITIC‐EDAS (the abbreviation for criteria importance through inter‐criteria correlation‐evaluation based on distance from average solution) evaluation approach is proposed. First, from the perspective of Weibo, an evaluation index system for the effectiveness of enterprise crisis management is constructed based on the analysis of the factors affecting the effectiveness of enterprise crisis management. Then, the CRITIC method is used to determine the weights of the evaluation index, and the EDAS method is utilized to calculate the enterprise crisis management effectiveness value based on the weights of the evaluation index. Finally, a case study about four enterprises' crisis events is applied to verify the feasibility of the CRITIC‐EDAS approach. Furthermore, some existing problems of enterprise crisis management are summarized and some strategies of enterprise crisis management for these problems are provided.