bluuebunny/crossref_metadata_2025_split huggingface dataset:
Lesion‐induced generation of interneuron cell types in specific dorsoventral domains in the spinal cord of adult zebrafish
Authors: Kuscha Veronika , Frazer Sarah L. , Dias Tatyana B. , Hibi Masahiko , Becker Thomas , Becker Catherina G. 
Published: April 2012

AbstractIn contrast to mammals, adult zebrafish regenerate neurons in the lesioned spinal cord. For example, motor neurons are generated from an olig2‐expressing population of pMN‐like ependymoradial glial cells in a ventrolateral position at the central canal. However, the extent of neuronal regeneration is unclear. Here we show, using a transgenic fish in which V2 interneurons are labeled by green fluorescent protein (GFP) under the control of the vsx1 promoter, that after a complete spinal cord transection, large numbers of V2 interneurons are generated in the vicinity of the lesion site. Tg(vsx1:GFP)+ cells are not present in the unlesioned spinal cord and label with the proliferation marker bromodeoxyuridine (BrdU) after a lesion. Some mediolaterally elongated Tg(vsx1:GFP)+ cells contact the central canal in a medial position. These cells likely arise from a p2‐like domain of ependymoradial glial progenitor cells, indicated by coexpression of Pax6 and Nkx6.1, but not DsRed driven by the olig2 promoter in these cells. We also present evidence that Pax2+ interneurons are newly generated after a spinal lesion, whereas the generation rate for a dorsal population of parvalbuminergic interneurons is comparatively low. Our results identify the regenerative potential of different interneuron types for the first time and support a model in which different progenitor cell domains in distinct dorsoventral positions around the central canal are activated by a lesion to give rise to diverse neuronal cell types in the adult zebrafish spinal cord. J. Comp. Neurol. 520:3604–3616, 2012. © 2012 Wiley Periodicals, Inc.
Neuronal populations in the basolateral nuclei of the amygdala are differentially increased in humans compared with apes: A stereological study
Authors: Barger Nicole , Stefanacci Lisa , Schumann Cynthia M. , Sherwood Chet C. , Annese Jacopo , Allman John M. , Buckwalter Joseph A. , Hof Patrick R. , Semendeferi Katerina 
Published: April 2012

AbstractIn human and nonhuman primates, the amygdala is known to play critical roles in emotional and social behavior. Anatomically, individual amygdaloid nuclei are connected with many neural systems that are either differentially expanded or conserved over the course of primate evolution. To address amygdala evolution in humans and our closest living relatives, the apes, we used design‐based stereological methods to obtain neuron counts for the amygdala and each of four major amygdaloid nuclei (the lateral, basal, accessory basal, and central nuclei) in humans, all great ape species, lesser apes, and one monkey species. Our goal was to determine whether there were significant differences in the number or percent of neurons distributed to individual nuclei among species. Additionally, regression analyses were performed on independent contrast data to determine whether any individual species deviated from allometric trends. There were two major findings. In humans, the lateral nucleus contained the highest number of neurons in the amygdala, whereas in apes the basal nucleus contained the highest number of neurons. Additionally, the human lateral nucleus contained 59% more neurons than predicted by allometric regressions on nonhuman primate data. Based on the largest sample ever analyzed in a comparative study of the hominoid amygdala, our findings suggest that an emphasis on the lateral nucleus is the main characteristic of amygdala specialization over the course of human evolution. J. Comp. Neurol. 520:3035–3054, 2012. © 2012 Wiley Periodicals, Inc.
Single‐Crystal X‐Ray Diffraction, Isolated‐Molecule and Cluster Electronic Structure Calculations, and Scanning Electron Microscopy in an Organic solid: Models for Intramolecular Motion in 4,4′‐Dimethoxybiphenyl
Authors: Wang Xianlong , Rotkina Lolita , Su Hong , Beckmann Peter A. 
Published: April 2012

AbstractThis paper brings together field emission scanning electron microscopy, single‐crystal X‐ray diffraction, and density functional electronic structure calculations in both an isolated molecule and a cluster of seven whole and fourteen half molecules of 4,4′‐dimethoxybiphenyl to investigate coupled methyl‐group rotation (over a barrier) and methoxy‐group libration (meaning a rotation from the ground state not all the way to the transition state and back again). The structure of the isolated molecule, determined by the electronic structure calculations, is compared with the structure of the molecule found in the crystal. As the methyl group rotates from its ground state to its transition state, the methoxy group rotates 30° in the isolated molecule and 16° in the cluster. The calculated barriers for this coupled methyl‐group rotation and methoxy group libration in the isolated molecule and in the crystal are 12.8 kJ mol−1 and 10.3 kJ mol−1 respectively, suggesting that intermolecular interactions in the crystal lower the barrier. These barriers are compared with the value of 11.5±0.5 kJ mol−1 obtained from solid‐state 1H spin‐lattice relaxation measurements [P. A. Beckmann and E. Schneider, J. Chem. Phys. 2012, 136, 054508.]
Interpersonal Psychotherapy for Postpartum Depression
Authors: Stuart Scott 
Published: April 2012

Perinatal depression is prevalent and has a great impact on both mother and infant. There are empirically validated treatments for both postpartum depression and depression during pregnancy. Primary among these is Interpersonal Psychotherapy, which has been shown to be effective for postpartum women across the spectrum from mild to severe depression. At present, Interpersonal Psychotherapy is the best validated treatment for postpartum depression and should be considered first‐line treatment, especially for depressed breastfeeding women. Copyright © 2012 John Wiley & Sons, Ltd.Key Practitioner Message

IPT is a first‐line evidence‐based treatment for postpartum depression.
IPT can be easily modified for postpartum depression.
Partners can be included in IPT for postpartum depression.
Group IPT for postpartum depression is effective for depression.
Nonlinear behavioural model of charge pump PLLs
Authors: Brambilla Angelo , Linaro Daniele , Storace Marco 
Published: April 2012

SUMMARYDespite the nonlinear nature of even the simplest versions of phase locked loops (PLLs), linear models are still used during the first phases of the design of modern PLLs. Even though the linear model may represent a crude approach, its use is justified by the fact that accurate numerical simulations often require a too large amount of CPU time, being PLLs by construction stiff circuits, characterised by very different time scales. This aspect has triggered the need for compact models that allow fast and accurate numerical simulations. The scientific literature numbers several models that have been developed with different approaches and tailored to different simulation environments. In this context, we propose a nonlinear model of a type‐II PLL, which (1) considers both the switching behaviour of the phase/frequency detector and charge pump and the complex dynamics (including the presence of amplitude and phase noise) of the voltage controlled oscillator, (2) is compact and can be easily implemented in modern mixed analog/digital simulators as a behavioural block, and (3) allows the simulation of spurs owing to the nonlinearities of both the charge pump and the fractional frequency divider. Copyright © 2012 John Wiley & Sons, Ltd.
Development and laboratory‐scale testing of a fully automated online flow cytometer for drinking water analysis
Authors: Hammes Frederik , Broger Tobias , Weilenmann Hans‐Ulrich , Vital Marius , Helbing Jakob , Bosshart Ulrich , Huber Pascal , Peter Odermatt Res , Sonnleitner Bernhard 
Published: April 2012

AbstractAccurate and sensitive online detection tools would benefit both fundamental research and practical applications in aquatic microbiology. Here, we describe the development and testing of an online flow cytometer (FCM), with a specific use foreseen in the field of drinking water microbiology. The system incorporated fully automated sampling and fluorescent labeling of bacterial nucleic acids with analysis at 5‐min intervals for periods in excess of 24 h. The laboratory scale testing showed sensitive detection (< 5% error) of bacteria over a broad concentration range (1 × 103–1 × 106 cells mL−1) and particularly the ability to track both gradual changes and dramatic events in water samples. The system was tested with bacterial pure cultures as well as indigenous microbial communities from natural water samples. Moreover, we demonstrated the possibility of using either a single fluorescent dye (e.g., SYBR Green I) or a combination of two dyes (SYBR Green I and Propidium Iodide), thus broadening the application possibilities of the system. The online FCM approach described herein has considerable potential for routine and continuous monitoring of drinking water, optimization of specific drinking water processes such as biofiltration or disinfection, as well as aquatic microbiology research in general. © 2012 International Society for Advancement of Cytometry
Intelligent route generation: discovery and search of correlation between shared resources
Authors: Yen Neil Y. , Huang Runhe , Ma Jianhua , Jin Qun , Shih Timothy K. 
Published: April 2012

SUMMARYSharing information and resources on the Internet has become an important activity for education. The use of ubiquitous devices makes it possible for learning participants to be engaged in an open and connected social environment, and also allows the learning activities to be performed at any time and any place. In this study, the discovery of correlation among shared resources is concentrated. A hypothetical scenario is considered that the information, such as photos and thoughts, is applicable to be shared with implicit context (i.e., geographical information) by learners through a practical implementation, PadSCORM, on a mobile device. Two major contributions are achieved. First, the correlations among resources are determined through usage experiences mining and geographical information adjustment. It then assists learners in filtering out redundant information by highlighting the significance of resources. Second, an intelligent searching algorithm is proposed to visualize adaptive routes to facilitate search process and to enrich the learning activity. The empirical experiments revealing the feasibility and performance (e.g., accuracy and effectiveness) are conducted in the universities in North Taiwan. Copyright © 2012 John Wiley & Sons, Ltd.
An authenticated group key distribution protocol based on the generalized Chinese remainder theorem
Authors: Guo Cheng , Chang Chin‐Chen 
Published: April 2012

SUMMARYThe group key distribution protocol is a mechanism for distributing a group key that is used to encrypt the communication data transmitted in an open group. Recently, a novel group key distribution protocol based on secret sharing was proposed. In their protocol, the group key information is broadcast in an open network environment, and only authorized group members can obtain the group key. However, their protocol requires each group member to broadcast a random challenge to the rest of the group members in the construction of the group key, and this may increase communication cost and cause network traffic congestion. In this paper, we propose an authenticated group key distribution protocol based on the generalized Chinese remainder theorem that drastically reduces communication costs while maintaining at least the same degree of security. Our protocol is built on the secret sharing scheme based on Chinese remainder theorem, which requires fewer computation operations than the previous work. Copyright © 2012 John Wiley & Sons, Ltd.
The Ly6 neurotoxin‐like molecule target of wit regulates spontaneous neurotransmitter release at the developing neuromuscular junction in Drosophila
Authors: Kim Nam Chul , Marqués Guillermo 
Published: April 2012

AbstractRefinement of neural circuits during development requires formation and elimination of synaptic connections, a process governed by activity‐dependent mechanisms and developmental genetic programs. Bone Morphogenetic Protein (BMP) retrograde signaling through the type II receptor Wishful thinking (Wit) is essential for synaptic growth and functional development of the Drosophila larval neuromuscular junction. However, little is known about the genes that are regulated by the pathway to effect synaptic growth and proper synaptic transmission. We have identified a transcriptional target of wit (twit), whose expression in motoneurons depends on Wit activity. Null twit mutants are viable and fertile, but recapitulate some of the electrophysiological phenotypes of wit mutants, such as decreased frequency and amplitude of miniature excitatory junction potentials. Other wit phenotypes, such as decreased synaptic terminal size and evoked excitatory junction potentials, are not found in twit mutants, suggesting that homeostatic compensation of presynaptic release is intact in twit mutants and that Wit regulates additional genes to accomplish proper synaptic maturation. Twit is a glycosylphosphatidylinositol‐anchored protein of the Lymphocyte antigen 6 family (Ly‐6), and neuronal expression of a twit transgene rescues twit's mutant phenotypes. Importantly, twit expression partially rescues diminished frequency of miniature excitatory synaptic potentials in wit mutants. This further supports the conclusions that Twit is signaling in larval motoneurons and an essential effector of the retrograde BMP signal. Taken together, our results suggest that retrograde BMP signaling regulates spontaneous neurotransmitter release by activating the transcription of the Ly‐6 gene twit. © 2011 Wiley Periodicals, Inc. Develop Neurobiol, 2012
Genetic mouse models for skin research: Strategies and resources
Authors: Schneider Marlon R. 
Published: April 2012

AbstractA number of features contributed to establishing the mouse as the favorite model organism for skin research: the genetic and pathophysiological similarities to humans, the small size and relatively short reproductive period, meaning low maintenance costs, and the availability of sophisticated tools for manipulating the genome, gametes, and embryos. While initial studies depended on strains displaying skin abnormalities due to spontaneous genetic mutations, the availability of the transgenic and knockout technologies and their astonishing perfection during the last decades allowed the development of mouse lines permitting any imaginable genetic modification including gene inactivation, substitution, modification, or overexpression. While these technologies have already contributed to the functional analysis of several genes and processes related to skin research, continued progress requires understanding, awareness, and access to these mouse resources. This review will identify the strategies currently employed for the genetic manipulation of mice in skin research, and outline current resources and their limitations. genesis 50:652–664, 2012. © 2012 Wiley Periodicals, Inc.
New Routes to Soluble Magnesium Amidoborane Complexes
Authors: Jones Cameron , Bonyhady Simon J. , Nembenna Sharanappa , Stasch Andreas 
Published: April 2012

AbstractSeveral new synthetic routes to the known and new magnesium amidoborane complexes, [{(Nacnac)Mg(NH2BH3)}2] and [(Nacnac)Mg(NH2BH3)(THF)] (Nacnac = DipNacnac, [{N(Dip)C(Me)}2CH]–, or tBuNacnac, [{N(Dip)C(tBu)}2CH]–; Dip = C6H3iPr2‐2,6), are reported. These include the reductive dehydrogenation reaction of NH3BH3 (AB) with the magnesium(I) dimer, [{(DipNacnac)Mg}2], and the salt elimination reaction of [(DipNacnac)MgI(OEt2)] with Li(NH2BH3). Moreover, the reaction of ammonia–borane with two magnesium(II) hydride complexes, [{(Nacnac)Mg(μ‐H)}2], or an alkylmagnesium(II) complex [{(DipNacnac)Mg(μ‐nBu)}2], have been shown to yield well defined parent amidoborane complexes of magnesium for the first time. The crystal structures of all prepared complexes were obtained and shown to be similar to those previously reported for related compounds.
Cholesterol photosensitized oxidation in muscle foods
Authors: Boselli Emanuele , Cardenia Vladimiro , Rodriguez‐Estrada Maria Teresa 
Published: April 2012

AbstractCholesterol photoxidation can affect the nutritional quality of fresh meat and fish due to the formation of cholesterol oxidation products (COPs), which exert a strong impact on the lipid metabolism and are involved in various chronic and degenerative diseases and disturbance of cell functionality. The evaluation of light sensitized cholesterol oxidation has been reviewed in beef, pork, horse, turkey, and sardine muscle. In the retail market, fresh slices of meat or fish are usually displayed in refrigerated vessels wrapped with plastic film and are exposed to a fluorescent light. Under these conditions, COPs can range from 8.5 µg/g of lipids (photoxidized pork meat) to more than 300 µg/g of lipids (photoxidized horse meat), corresponding to a maximum of 1.3% of oxidized cholesterol. The oxidative process can be reduced by feeding the animals with antioxidants, such as tocopherols, or by spraying the muscle food with lipid‐ or water‐soluble antioxidants before packaging. However, the combined use of alternative protective packaging and lighting conditions during commercial retail storage, such as the use of red wrapping films, warm tone lamps, and modified atmosphere with low oxygen content, can efficiently prevent photoxidation without modifying the food product composition and sensory properties.
Sensitive and Selective Sensing of Hydrogen Peroxide with Iron‐Tetrasulfophthalocyanine‐Graphene‐Nafion Modified Screen‐Printed Electrode
Authors: Zhu Mingfang , Li Nan , Ye Jianshan 
Published: April 2012

AbstractWe developed a novel iron‐tetrasulfophthalocyanine‐graphene‐Nafion (FeTSPc‐GR‐Nafion) modified screen‐printed electrode to determine hydrogen peroxide (H2O2) with high sensitivity and selectivity. The nanocomposite film (FeTSPc‐GR‐Nafion) exhibits an excellent electrocatalytic activity towards oxidation of H2O2 at a potential of +0.35 V in the absence of enzyme. A comparative study reveals that the FeTSPc‐GR complexes play a dual amplification role. Amperometric experiment indicates that the sensors possess good sensitivity and selectivity, with a linear range from 2.0×10−7 M to 5.0×10−3 M and a detection limit of 8.0×10−8 M. This sensor has been successfully used to develop the glucose biosensor and has also been applied to determine H2O2 in sterile water.
Model‐based predicting and correcting algorithms for substructure online hybrid tests
Authors: Wang Tao , Cheng Chun , Guo Xun 
Published: April 2012

SUMMARYA set of algorithms combined with a substructure technique is proposed for an online hybrid test framework, in which the substructures are encapsulated by a standard interface that implements displacements and forces at the common substructure boundaries. A coordinator equipped with the proposed algorithms is designed to achieve boundary compatibility and equilibrium, thereby endowing the substructures the ability to behave as one piece. A model‐based predictor and corrector, and a noniterative procedure, characterize the set of algorithms. The coordinator solves the dynamics of the entire structure and updates the static boundary state simultaneously by a quasi‐Newton procedure, which gradually formulates the condensed stiffness matrix associated with corresponding degrees of freedom. With the condensed stiffness matrix and dynamic information, a condensed equation of motion is derived and then solved by a typical time integration algorithm. Three strategies for updating the condensed stiffness matrix are incorporated into the proposed algorithms. Each adopts different stiffness matrix during the predicting and correcting stage. These algorithms are validated by two numerical substructure simulations and a hybrid test. The effectiveness and feasibility are fully demonstrated. Copyright © 2012 John Wiley & Sons, Ltd.
Quantifying the anthropogenic fraction of fatty alcohols in a terrestrial environment
Authors: Mudge Stephen M. , DeLeo Paul C. , Dyer Scott D. 
Published: April 2012

Abstract
Fatty alcohols are naturally produced hydrocarbons present in all living organisms. They are also used in detergent and cosmetic formulations, may be sourced from either petroleum or biological materials, and are typically disposed of down the drain. This study was conducted on the Luray catchment, Virginia, USA, where sales data indicate that approximately 2 kg of fatty alcohols from detergent enter the wastewater every day. Reconstructing fatty alcohols in the influent on the basis of sales data indicated a mix of odd and even chain compounds, with C12 being dominant. This profile was influenced strongly by liquid laundry detergents (69%). Sediment and soil samples from the catchment were analyzed by gas chromatography–mass spectrometry and by stable isotope ratio mass spectrometry to determine the δ13C and δ2H signatures. The long-chain components in agricultural soils and river sediments were distinguishable clearly from the algal fatty alcohols produced within the river system. The wastewater was a mixture of fecal and detergent sources of fatty alcohols in a ratio of 75:25%. The fatty alcohols in the effluent had different stable isotopic signatures and chain-length profiles from the influent, indicating that these compounds are not the same as those that entered the treatment plant. The total quantity of fatty alcohols leaving the treatment plant through the effluent pipe was low compared with the input. Analysis of the contributions based on the stable isotopes and profiles suggests that of the fatty alcohols present in the river system downstream of the treatment plant, 84% were derived from terrestrial plant production, 15% came from in situ algal synthesis, and 1% were derived from the effluent. Environ. Toxicol. Chem. 2012;31:1209–1222. © 2012 SETAC