bluuebunny/crossref_metadata_2025_split huggingface dataset:
Production of extracellular fatty acid using engineered Escherichia coli
Authors: Liu Hui , Yu Chao , Feng Dexin , Cheng Tao , Meng Xin , Liu Wei , Zou Huibin , Xian Mo 
Published: April 2012

Abstract

Background
As an alternative for economic biodiesel production, the microbial production of extracellular fatty acid from renewable resources is receiving more concerns recently, since the separation of fatty acid from microorganism cells is normally involved in a series of energy-intensive steps. Many attempts have been made to construct fatty acid producing strains by targeting genes in the fatty acid biosynthetic pathway, while few studies focused on the cultivation process and the mass transfer kinetics.

Results
In this study, both strain improvements and cultivation process strategies were applied to increase extracellular fatty acid production by engineered Escherichia coli. Our results showed overexpressing ‘TesA and the deletion of fadL in E. coli BL21 (DE3) improved extracellular fatty acid production, while deletion of fadD didn’t strengthen the extracellular fatty acid production for an undetermined mechanism. Moreover, the cultivation process controls contributed greatly to extracellular fatty acid production with respect to titer, cell growth and productivity by adjusting the temperature, adding ampicillin and employing on-line extraction. Under optimal conditions, the E. coli strain (pACY-‘tesA-ΔfadL) produced 4.8 g L−1 extracellular fatty acid, with the specific productivity of 0.02 g h−1 g−1dry cell mass, and the yield of 4.4% on glucose, while the ratios of cell-associated fatty acid versus extracellular fatty acid were kept below 0.5 after 15 h of cultivation. The fatty acids included C12:1, C12:0, C14:1, C14:0, C16:1, C16:0, C18:1, C18:0. The composition was dominated by C14 and C16 saturated and unsaturated fatty acids. Using the strain pACY-‘tesA, similar results appeared under the same culture conditions and the titer was also much higher than that ever reported previously, which suggested that the supposedly superior strain did not necessarily perform best for the efficient production of desired product. The strain pACY-‘tesA could also be chosen as the original strain for the next genetic manipulations.

Conclusions
The general strategy of metabolic engineering for the extracellular fatty acid production should be the cyclic optimization between cultivation performance and strain improvements. On the basis of our cultivation process optimization, strain improvements should be further carried out for the effective and cost-effective production process.
Acute effects of aerobic exercise on mood and hunger feelings in male obese adolescents: a crossover study
Authors: Lofrano-Prado Mara Cristina , Hill James O , Silva Humberto José Gomes , Freitas Camila Rodrigues Menezes , Lopes-de-Souza Sandra , Lins Tatiana Acioli , do Prado Wagner Luiz 
Published: April 2012

Abstract

Background
The aim of this study was to determine the acute effects of exercise intensity on anxiety, mood states and hunger in obese adolescents.

Methods
Subjects were eight male obese adolescents (age 15.44 ± 2.06y; BMI 33.06 ± 4.78 kg/m2). Each subject underwent three experimental trials: 1) Control, seated for 30 min; 2) Low intensity exercise (LIE) - exercise at 10% below ventilatory threshold (VT); 3) High intensity exercise (HIE) - exercise at 10% above VT. Anxiety (STAI Trait/State), mood (POMS) and hunger (VAS) were assessed before and immediately after the experimental sessions. Comparisons between trials and times were assessed using Kruskal-Wallis and Wilcoxon tests, respectively. Associations between variables were described using a Spearman test.

Results
The largest increase in hunger was observed after LEI (914.22%). Both exercise sessions increased anxiety, fatigue and decreased vigor (p < 0.05).

Conclusions
Acute exercise bouts are associated with negative changes in anxiety and mood, and with increases in hunger in obese adolescents.
Longitudinal, cross-cohort comparison of physical activity patterns in Chinese mothers and children
Authors: Dearth-Wesley Tracy , Gordon-Larsen Penny , Adair Linda S , Zhang Bing , Popkin Barry M 
Published: April 2012

AbstractBackgroundThere is limited evidence comparing adult and child physical activity (PA) trends and examining parent–child PA associations within a newly industrialized country setting. PA research within a newly industrialized country setting is particularly important given the negative effects of rapid urbanization, socioeconomic growth, and technological advances on PA behaviors. The purpose of our study was to examine trends and associations in PA behaviors in Chinese mother-child pairs and to investigate relationships between PA behaviors and socioeconomic variables in this dyad.MethodsWe studied PA behaviors in 2 separate cohorts of mother-child pairs (n = 353) followed over a 2–4 year time period using longitudinal data from the China Health and Nutrition Survey (2000 Cohort: 2000–2004; 2004 Cohort: 2004–2006). Comparable mother-child PA behaviors included total metabolic equivalent hours per week (MET-hrs/wk) from active commuting, leisure-time sports, and sedentary behaviors. Logistic regression models were used to examine associations between mother and child PA and relationships between PA behaviors and socioeconomic variables.ResultsChildren experienced increases in active commuting and leisure-time sports activities with increasing child age, whereas mothers experienced temporal declines in active commuting and minimal change in leisure-time sports activity. Sedentary behavior was high for children and mothers over time. Mother-child associations were positive for active commuting and leisure-time sports activities and negative for sedentary behavior (P < 0.05). Maternal education was associated with a greater likelihood of high leisure-time sports activity and high sedentary behavior in mothers but not in children (P < 0.05).ConclusionEfforts to reduce sedentary behavior in Chinese mothers and children are imperative. While increased leisure-time and active commuting activities in children is encouraging, continued PA promotion in children and more intensive efforts to promote leisure-time sports and active commuting in mothers is needed.
Surface-wave analysis for identifying unfrozen zones in subglacial sediments
Authors: Tsuji Takeshi , Johansen Tor Arne , Ruud Bent Ole , Ikeda Tatsunori , Matsuoka Toshifumi 
Published: April 2012

To reveal the extent of freezing in subglacial sediments, we estimated S-wave velocity along a glacier using surface-wave analysis. Because the S-wave velocity varies significantly with the degree of freezing of the pore fluid in the sediments, this information is useful for identifying unfrozen zones within subglacial sediments, which again is important for glacier dynamics. We used active-source multichannel seismic data originally acquired for reflection analysis along a glacier at Spitsbergen in the Norwegian Arctic and proposed an effective approach of multichannel analysis of surface waves (MASW) in a glacier environment. Common-midpoint crosscorrelation gathers were used for the MASW to improve lateral resolution because the glacier bed has a rough topology. We used multimode analysis with a genetic algorithm inversion to estimate the S-wave velocity due to the potential existence of a low-velocity layer beneath the glacier ice and the observation of higher modes in the dispersion curves. In the inversion, we included information of ice thickness derived from high-resolution ground-penetrating radar data because a simulation study demonstrated that the ice thickness was necessary to estimate accurate S-wave velocity distribution of deep subglacial sediment. The estimated S-wave velocity distribution along the seismic line indicated that low velocities occurred below the glacier, especially beneath thick ice ([Formula: see text] for ice thicknesses larger than 50 m). Because this velocity was much lower than the velocity in pure ice ([Formula: see text]), the pore fluid was partially melted at the ice–sediment interface. At the shallower subglacial sediments (ice thickness less than 50 m), the S-wave velocity was similar to that of the pure ice, suggesting that shallow subglacial sediments are more frozen than sediments beneath thick ice.
Outcome of Acute Promyelocytic Leukemia (APL) in Children and Adolescents: An Analysis in Two Consecutive Trials of the European APL Group
Authors: Bally Cecile , Fadlallah Jehane , Leverger Guy , Bertrand Yves , Robert Alain , Baruchel Andre , Guerci Agnes , Recher Christian , Raffoux Emmanuel , Thomas Xavier , Leblanc Thierry , Idres Nadia , Cassinat Bruno , Vey Norbert , Chomienne Christine , Dombret Herve , Sanz Miguel , Fenaux Pierre , Adès Lionel 
Published: April 2012

Purpose Acute promyelocytic leukemia (APL) is rare in children. All-trans-retinoic acid (ATRA) combined with chemotherapy, the reference treatment of APL, is generally considered to produce similar results in children and adults. However, previously published childhood APL studies have generally analyzed all patients age < 18 years as a group, without further dividing according to age. Patients and Methods We compared disease characteristics and outcomes of children (age ≤ 12 years), adolescents (13 to 18 years), and adults (> 18 years) included in two multicenter APL clinical trials (APL 93 and 2000 trials). Results Of the 833 patients age ≤ 60 years included in the two trials, 26 (3%), 58 (7%), and 749 (90%) were children, adolescents, and adults, respectively. Children had significantly higher baseline WBC counts (P < .001). The complete remission (CR) rate (92%, 100%, and 94.5%, respectively) and 5-year cumulative incidence of relapse (CIR; 28%, 20%, and 23%, respectively) did not differ between children, adolescents, and adults, whereas adolescents had significantly better overall survival (OS; 5-year OS, 93.6% v 80.4% in adults and 80.4% in children; P = .03). However, in children age ≤ 4 years, the 5-year CIR was 52%, compared with 17.6% in children age 5 to 12 years (P = .006), although most of the younger children who relapsed experienced durable salvage with autologous or allogeneic stem-cell transplantation. Conclusion Adolescents and children age > 4 years with APL treated with ATRA and chemotherapy have outcomes at least as favorable as those of adults. Younger children seem to experience more relapses and may require reinforcement of first-line treatment.
Influence of Major Postoperative Complications on Health-Related Quality of Life Among Long-Term Survivors of Esophageal Cancer Surgery
Authors: Derogar Maryam , Orsini Nicola , Sadr-Azodi Omid , Lagergren Pernilla 
Published: April 2012

Purpose To evaluate the effect of major postoperative complications on health-related quality of life (HRQL) in 5-year survivors of esophageal cancer surgery. Patients and Methods This study was based on the Swedish Esophageal and Cardia Cancer register with almost complete nationwide coverage and data on esophageal cancer surgery collected prospectively between 2001 and 2005. Patients who were alive 5 years after surgery were eligible. HRQL was assessed longitudinally until 5 years after surgery by using the validated European Organisation for Research and Treatment of Cancer (EORTC) Quality of Life Questionnaire C30 and OES18. Linear mixed models were used to assess the mean score difference (MD) with 95% CIs of each aspect of HRQL in patients with or without major postoperative complications. Adjustment was made for several potential confounders. Results Of 153 patients who survived 5 years, 141 patients (92%) answered the 5-year HRQL questionnaires. Of these individuals, 46 patients (33%) sustained a major postoperative complication. Dyspnea (MD, 15; 95% CI, 6 to 23), fatigue (MD, 13; 95% CI, 5 to 20), and eating restrictions (MD, 10; 95% CI, 2 to 17) were clinically and statistically significantly deteriorated throughout the follow-up in patients with major postoperative complications compared with patients without major complications. Although problems with choking declined to levels comparable with patients without major postoperative complications, sleep difficulties and gastroesophageal reflux progressively worsened during follow-up. Conclusion The occurrence of postoperative complications exerts a long-lasting negative effect on HRQL in patients who survive 5 years after esophagectomy for cancer.
Maternal Caffeine Consumption and Infant Nighttime Waking: Prospective Cohort Study
Authors: Santos Iná S. , Matijasevich Alicia , Domingues Marlos R. 
Published: April 2012

OBJECTIVE:
Coffee and other caffeinated beverages are commonly consumed in pregnancy. In adults, caffeine may interfere with sleep onset and have a dose-response effect similar to those seen during insomnia. In infancy, nighttime waking is a common event. With this study, we aimed to investigate if maternal caffeine consumption during pregnancy and lactation leads to frequent nocturnal awakening among infants at 3 months of age.

METHODS:
All children born in the city of Pelotas, Brazil, during 2004 were enrolled on a cohort study. Mothers were interviewed at delivery and after 3 months to obtain information on caffeine drinking consumption, sociodemographic, reproductive, and behavioral characteristics. Infant sleeping pattern in the previous 15 days was obtained from a subsample. Night waking was defined as an episode of infant arousal that woke the parents during nighttime. Multivariable analysis was performed by using Poisson regression.

RESULTS:
The subsample included 885 of the 4231 infants born in 2004. All but 1 mother consumed caffeine in pregnancy. Nearly 20% were heavy consumers (≥300 mg/day) during pregnancy and 14.3% at 3 months postpartum. Prevalence of frequent nighttime awakeners (&gt;3 episodes per night) was 13.8% (95% confidence interval: 11.5%–16.0%). The highest prevalence ratio was observed among breastfed infants from mothers consuming ≥300 mg/day during the whole pregnancy and in the postpartum period (1.65; 95% confidence interval: 0.86–3.17) but at a nonsignificant level.

CONCLUSIONS:
Caffeine consumption during pregnancy and by nursing mothers seems not to have consequences on sleep of infants at the age of 3 months.
Relationship Between Maternal and Neonatal Staphylococcus aureus Colonization
Authors: Jimenez-Truque Natalia , Tedeschi Sara , Saye Elizabeth J. , McKenna Brian D. , Langdon Weston , Wright Jesse P. , Alsentzer Andrew , Arnold Sandra , Saville Benjamin R. , Wang Wenli , Thomsen Isaac , Creech C. Buddy 
Published: April 2012

OBJECTIVE:
The study aimed to assess whether maternal colonization with Staphylococcus aureus during pregnancy or at delivery was associated with infant staphylococcal colonization.

METHODS:
For this prospective cohort study, women were enrolled at 34 to 37 weeks of gestation between 2007 and 2009. Nasal and vaginal swabs for culture were obtained at enrollment; nasal swabs were obtained from women and their infants at delivery and 2- and 4-month postbirth visits. Logistic regression was used to determine whether maternal colonization affected infant colonization.

RESULTS:
Overall, 476 and 471 mother-infant dyads had complete data for analysis at enrollment and delivery, respectively. Maternal methicillin-resistant S aureus (MRSA) colonization occurred in 10% to 17% of mothers, with the highest prevalence at enrollment. Infant MRSA colonization peaked at 2 months of age, with 20.9% of infants colonized. Maternal staphylococcal colonization at enrollment increased the odds of infant staphylococcal colonization at birth (odds ratio; 95% confidence interval: 4.8; 2.4–9.5), hospital discharge (2.6; 1.3–5.0), at 2 months of life (2.7; 1.6–4.3), and at 4 months of life (2.0; 1.1–3.5). Similar results were observed for maternal staphylococcal colonization at delivery. Fifty maternal-infant dyads had concurrent MRSA colonization: 76% shared isolates of the same pulsed-field type, and 30% shared USA300 isolates. Only 2 infants developed staphylococcal disease.

CONCLUSIONS:
S aureus colonization (including MRSA) was extremely common in this cohort of maternal-infant pairs. Infants born to mothers with staphylococcal colonization were more likely to be colonized, and early postnatal acquisition appeared to be the primary mechanism.
Innate Immune Receptor Expression in Peri‐Implant Tissues of Patients With Different Susceptibility to Periodontal Diseases
Authors: Koutouzis Theofilos , Catania Dominick , Neiva Kathleen , Wallet Shannon M. 
Published: April 2012

Background: Although inflammation mediates the pathogenesis of periodontal diseases, the effects of innate immune responses on implant therapies have not been evaluated. Innate immune receptors, including toll‐like‐receptors (TLRs) and the receptor for advanced glycated end‐products (RAGE), are upregulated within inflamed gingiva and are responsible for initiation of detrimental host responses. The aim of this study is to compare the expression of TLR2, TLR4, and RAGE in gingival tissues from participants susceptible to periodontitis and participants not susceptible to periodontitis before and after implant therapy.Methods: Periodontally healthy participants received implant therapy for non‐periodontal edentulism. Participants susceptible to periodontitis were diagnosed with chronic periodontitis prior to implant therapy. Gingival biopsies were collected from edentulous ridges before implant installation and from peri‐implant mucosa 2 months after treatment. Histology, real‐time PCR, and Western blot were used to evaluate levels of inflammatory infiltrate, TLR2, TLR4, and RAGE expression.Results: Before implant therapy, elevated levels of RAGE were detected in gingival tissues from participants susceptible to periodontitis when compared to those from participants with healthy periodontiums, whereas no differences in the expression of TLR2 or TLR4 were detected. After implant therapy, there was an upregulation of RAGE and TLR4 levels that coincided with a downregulation of TLR2 levels in biopsies from participants susceptible to periodontitis. Levels of RAGE and TLR4 remained unchanged in biopsies from participants with healthy periodontiums, whereas TLR2 levels were significantly upregulated. Histologically, post‐implant biopsies from participants susceptible to periodontitis displayed higher levels of inflammatory infiltrate.Conclusion: Elevated levels of inflammatory potential were found after implant therapy in participants susceptible to periodontitis.
Enhancing Auditors' Ability to Identify Opportunities to Commit Fraud: Instructional Resource Cases
Authors: Dickins Denise , Reisch John T. 
Published: April 2012

ABSTRACT
Research suggests that audit team members, especially staff, may lack the necessary training to be able to identify fraud risks and to develop appropriate responses to identified fraud risks (e.g., Pearson and Singleton 2008; Knapp and Knapp 2001; Braun 2000; Zimbelman 1997). This lack of training may be particularly acute with respect to the perpetration of fraud in automated environments (Filipek 2007). The case studies are designed to improve undergraduate auditing, accounting information systems, and forensic accounting students' abilities to recognize and respond to indicators of fraud and general information technology (IT) control weaknesses.
Microsatellite markers isolated from the wild medicinal plant Centella asiatica (Apiaceae) from an enriched genomic library
Authors: Rakotondralambo Soaharin'ny Ony Raoseta , Lussert Alexandra , Rivallan Ronan , Danthu Pascal , Noyer Jean‐Louis , Baurens Franc‐Christophe 
Published: April 2012

• Premise of the study: Microsatellite markers for Centella asiatica, an important medicinal herb, were developed and characterized to promote genetic and molecular studies.• Methods and Results: A GA/GT‐enriched genomic library was constructed from an accession from Madagascar. Roughly 75% of the 768 clones of the enriched library contained microsatellites. Eighty sequences containing microsatellites were obtained from 96 positive clones. Specific primers were designed for 20 loci, and 17 of them displayed polymorphism when screened across 17 C. asiatica accessions, with an average of 4.3 alleles per locus. The observed and expected heterozygosity values averaged 0.114 and 0.379, respectively.• Conclusions: This is the first report constructing an enriched genomic library and identifying microsatellite markers from C. asiatica. These 17 polymorphic microsatellite markers are a useful resource for this plant, applicable for diversity studies, pedigree analyses, and genetic mapping.
High Frequency of HIV Mutations Associated with HLA-C Suggests Enhanced HLA-C–Restricted CTL Selective Pressure Associated with an AIDS-Protective Polymorphism
Authors: Blais Marie-Eve , Zhang Yonghong , Rostron Tim , Griffin Harry , Taylor Stephen , Xu Keyi , Yan Huiping , Wu Hao , James Ian , John Mina , Dong Tao , Rowland-Jones Sarah L. 
Published: April 2012

Abstract
Delayed HIV-1 disease progression is associated with a single nucleotide polymorphism upstream of the HLA-C gene that correlates with differential expression of the HLA-C Ag. This polymorphism was recently shown to be a marker for a protective variant in the 3′UTR of HLA-C that disrupts a microRNA binding site, resulting in enhanced HLA-C expression at the cell surface. Whether individuals with “high” HLA-C expression show a stronger HLA-C–restricted immune response exerting better viral control than that of their counterparts has not been established. We hypothesized that the magnitude of the HLA-C–restricted immune pressure on HIV would be greater in subjects with highly expressed HLA-C alleles. Using a cohort derived from a unique narrow source epidemic in China, we identified mutations in HIV proviral DNA exclusively associated with HLA-C, which were used as markers for the intensity of the immune pressure exerted on the virus. We found an increased frequency of mutations in individuals with highly expressed HLA-C alleles, which also correlated with IFN-γ production by HLA-C–restricted CD8+ T cells. These findings show that immune pressure on HIV is stronger in subjects with the protective genotype and highlight the potential role of HLA-C–restricted responses in HIV control. This is, to our knowledge, the first in vivo evidence supporting the protective role of HLA-C–restricted responses in nonwhites during HIV infection.