bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prolactin directly enhanced Na+/K+- and Ca2+-ATPase activities in the duodenum of female rats
Authors: Charoenphandhu Narattaphol , Limlomwongse Liangchai , Krishnamra Nateetip 
Published: October 2010

Prolactin has recently been shown to directly stimulate 2 components of the active duodenal calcium transport in female rats, i.e., solvent drag-induced and transcellular-active calcium transport. Since the basolateral Na+/K+- and Ca2+-ATPases, respectively, play important roles in these 2 transport mechanisms, the present study aimed to examine the direct actions of prolactin on the activities of both transporters in sexually mature female Wistar rats. The results showed that 200, 400, and 800 ng/mL prolactin produced a significant increase in the total ATPase activity of duodenal crude homogenate in a dose-dependent manner within 60 min (i.e., from a control value of 1.53 ± 0.13 to 2.29 ± 0.21 (p < 0.05), 2.68 ± 0.19 (p < 0.01), and 3.92 ± 0.33 (p < 0.001) µmol Pi·(mg protein)–1·min–1, respectively). Activity of Na+/K+-ATPase was increased by 800 ng/mL prolactin from 0.17 ± 0.03 to 1.18 ± 0.29 µmol Pi·(mg protein)–1·min–1(p < 0.01). Prolactin at doses of 400 and 600 ng/mL also significantly increased the activities of Ca2+-ATPase in crude homogenate from a control value of 0.84 ± 0.03 to 1.75 ± 0.29 (p < 0.05), and 2.30 ± 0.37 (p < 0.001) µmol Pi·(mg protein)–1·min–1. When the crude homogenate was purified for the basolateral membrane, the Na+/K+-ATPase activities were elevated 10-fold. In the purified homogenate, 800 ng/mL prolactin increased Na+/K+-ATPase activity from 1.79 ± 0.38 to 2.63 ± 0.44 µmol Pi·(mg protein)–1·min–1(p < 0.05), and Ca2+-ATPase activity from 0.08 ± 0.14 to 2.03 ± 0.23 µmol Pi·(mg protein)–1·min–1(p < 0.001). Because the apical calcium entry was the first important step for the transcellular active calcium transport, the brush border calcium uptake was also investigated in this study. We found that, 8 min after being directly exposed to 800 ng/mL prolactin, the brush border calcium uptake into the duodenal epithelial cells was increased from 0.31 ± 0.02 to 0.80 ± 0.28 nmol·(mg protein)–1(p < 0.05). It was concluded that prolactin directly and rapidly enhanced the brush border calcium uptake as well as the activities of the basolateral Na+/K+- and Ca2+-ATPases in the duodenal epithelium of female rats. These findings explained the mechanisms by which prolactin stimulated duodenal active calcium absorption.
Long-duration freewheel running and submandibular lymphocyte response to forced exercise in older mice
Authors: Boudreau J. , Hoffman-Goetz L. 
Published: October 2010

Submandibular lymph nodes (SLN) are crucial for immune surveillance of the anterior ocular chamber and upper respiratory tract; little is known about how training and exercise affect SLN lymphocytes. The intent of this study was to describe the impact of long term freewheel running followed by acute strenuous exercise on SLN lymphocytes in mice. Female C57BL/6 mice were assigned to running wheels or remained sedentary for 8 months, and further randomized to treadmill exercise and sacrifice immediately, treadmill exercise and sacrifice 24 h after exercise cessation, or no treadmill exposure. SLN lymphocytes were isolated and analyzed for CD3, CD4, CD8, and CD19 cell surface markers, phosphatidylserine externalization as a marker of apoptosis, and intracellular glutathione as a marker of oxidative stress. Compared with running wheel mice, older sedentary mice had a lower percent of T cells and higher percent of B cells (p < 0.05). Although intracellular glutathione did not differ between groups, running mice had a lower percent of Annexin V+ SLN lymphocytes 24 h after treadmill exercise. Further research will be needed to determine if voluntary exercise translates into improved anterior ocular and upper respiratory tract health.
Insulin Receptor Substrate Regulation of Phosphoinositide 3-Kinase
Authors: Metz Heather E. , McGarry Houghton A. 
Published: October 2010

Abstract
Insulin receptor substrates (IRS) serve as downstream messengers from activated cell surface receptors to numerous signaling pathway cascades. One of these pathways, phosphoinositide 3-kinase (PI3K), frequently displays aberrant function in the setting of cancer. IRS proteins are capable of both regulating and activating PI3K, depending on the cell of origin. As such, both prohost and protumor functions have been described for IRS proteins in human cancers. IRS proteins may eventually serve as biomarkers of PI3K activity, and serve a much-needed role as a guide to using targeted pathway therapy. Additionally, IRS-1 could be indirectly targeted in lung cancer, by inhibiting neutrophil elastase, which functions to degrade IRS-1 in lung tumor cells, thereby generating PI3K hyperactivity. Clin Cancer Res; 17(2); 206–11. ©2010 AACR. Clin Cancer Res; 17(2); 206–11. ©2010 AACR.
Role of Toll-Like Receptor 4 in Intimal Foam Cell Accumulation in Apolipoprotein E–Deficient Mice
Authors: Higashimori Mie , Tatro Jeffrey B. , Moore Kathryn J. , Mendelsohn Michael E. , Galper Jonas B. , Beasley Debbie 
Published: October 2010

Objective—
Atherosclerosis encompasses a conspicuously maladaptive inflammatory response that might involve innate immunity. Here, we compared the role of Toll-like receptor 4 (TLR4) with that of TLR2 in intimal foam cell accumulation and inflammation in apolipoprotein E (ApoE) knockout (KO) mice in vivo and determined potential mechanisms of upstream activation and downstream action.

Methods and Results—
We measured lipid accumulation and gene expression in the lesion-prone lesser curvature of the aortic arch. TLR4 deficiency reduced intimal lipid by ≈75% in ApoE KO mice, despite unaltered total serum cholesterol and triglyceride levels, whereas TLR2 deficiency reduced it by ≈45%. TLR4 deficiency prevented the increased interleukin-1α (IL-1α) and monocyte chemoattractant protein-1 mRNA levels seen within lesional tissue, and it also lowered serum IL-1α levels. Smooth muscle cells (SMC) were present within the intima of the lesser curvature of the aortic arch at this early lesion stage, and they enveloped and permeated nascent lesions, which consisted of focal clusters of foam cells. Cholesterol enrichment of SMC in vitro stimulated acyl-coenzyme A:cholesterol acyltransferase-1 mRNA expression, cytoplasmic cholesterol ester accumulation, and monocyte chemoattractant protein-1 mRNA and protein expression in a TLR4-dependent manner.

Conclusion—
TLR4 contributes to early-stage intimal foam cell accumulation at lesion-prone aortic sites in ApoE KO mice, as does TLR2 to a lesser extent. Intimal SMC surround and penetrate early lesions, where TLR4 signaling within them may influence lesion progression.
Macrophage, But Not Systemic, Apolipoprotein E Is Necessary for Macrophage Reverse Cholesterol Transport In Vivo
Authors: Zanotti Ilaria , Pedrelli Matteo , Potì Francesco , Stomeo Grazia , Gomaraschi Monica , Calabresi Laura , Bernini Franco 
Published: October 2010

Objective—
To assess the role of apolipoprotein (apo) E in macrophage reverse cholesterol transport (RCT) in vivo.

Methods and Results—
ApoE exerts an antiatherosclerotic activity by regulating lipoprotein metabolism and promoting cell cholesterol efflux. We discriminated between macrophage and systemic apoE contribution using an assay of macrophage RCT in mice. The complete absence of apoE lead to an overall impairment of the process and, similarly, the absence of apoE exclusively in macrophages resulted in the reduction of cholesterol mobilization from macrophages to plasma, liver, and feces. Conversely, expression of apoE in macrophages is sufficient to promote normal RCT even in apoE-deficient mice. The mechanisms accounting for these results were investigated by evaluating the first step of RCT (ie, cholesterol efflux from cells). Macrophages isolated from apoE-deficient mice showed a reduced ability to release cholesterol into the culture medium, whereas the apoB-depleted plasma from apoE-deficient and healthy mice possessed a similar capacity to promote cellular lipid release from cultured macrophages.

Conclusion—
Our data demonstrate, for the first time to our knowledge, that apoE significantly contributes to macrophage RCT in vivo and that this role is fully attributable to apoE expressed in macrophages.
Binding Preferences for GPIHBP1, a Glycosylphosphatidylinositol-Anchored Protein of Capillary Endothelial Cells
Authors: Gin Peter , Beigneux Anne P. , Voss Constance , Davies Brandon S.J. , Beckstead Jennifer A. , Ryan Robert O. , Bensadoun André , Fong Loren G. , Young Stephen G. 
Published: October 2010

Objective—
To define the ability of GPIHBP1 to bind other lipase family members and other apolipoproteins (apos) and lipoproteins.

Methods and Results—
GPIHBP1, a GPI-anchored lymphocyte antigen (Ly)6 protein of capillary endothelial cells, binds lipoprotein lipase (LPL) avidly, but its ability to bind related lipase family members has never been evaluated. As judged by cell-based and cell-free binding assays, LPL binds to GPIHBP1, but other members of the lipase family do not. We also examined the binding of apoAV-phospholipid disks to GPIHBP1. ApoAV binds avidly to GPIHBP1-transfected cells; this binding requires GPIHBP1's amino-terminal acidic domain and is independent of its cysteine-rich Ly6 domain (the latter domain is essential for LPL binding). GPIHBP1-transfected cells did not bind high-density lipoprotein. Chylomicrons bind avidly to GPIHBP1-transfected Chinese hamster ovary cells, but this binding is dependent on GPIHBP1's ability to bind LPL within the cell culture medium.

Conclusion—
GPIHBP1 binds LPL but does not bind other lipase family members. GPIHBP1 binds apoAV but does not bind apoAI or high-density lipoprotein. The ability of GPIHBP1-transfected Chinese hamster ovary cells to bind chylomicrons is mediated by LPL; chylomicron binding does not occur unless GPIHBP1 first captures LPL from the cell culture medium.
The Anti-Inflammatory Cytokine Interleukin 19 Is Expressed By and Angiogenic for Human Endothelial Cells
Authors: Jain Surbhi , Gabunia Khatuna , Kelemen Sheri E. , Panetti Tracee S. , Autieri Michael V. 
Published: October 2010

Objective—
To characterize the expression and function of interleukin (IL) 19, a recently described T-helper 2 anti-inflammatory IL, on endothelial cell (EC) pathophysiological features.

Methods and Results—
The expression and effects of anti-inflammatory ILs on EC activation and development of angiogenesis are uncharacterized. We demonstrate by immunohistochemistry and immunoblot that IL-19 is expressed in inflamed, but not normal, human coronary endothelium and can be induced in cultured human ECs by serum and basic fibroblast growth factor. IL-19 is mitogenic and chemotactic, and it promotes EC spreading. IL-19 activates the signaling proteins STAT3, p44/42, and Rac1. In functional ex vivo studies, IL-19 promotes cordlike structure formation of cultured ECs and enhances microvessel sprouting in the mouse aortic ring assay. IL-19 induces tube formation in gelatinous protein (Matrigel) plugs in vivo.

Conclusion—
To our knowledge, these data are the first to report expression of the anti-inflammatory agent, IL-19, in ECs; and the first to indicate that IL-19 is mitogenic and chemotactic for ECs and can induce the angiogenic potential of ECs.
S100A12 in Vascular Smooth Muscle Accelerates Vascular Calcification in Apolipoprotein E–Null Mice by Activating an Osteogenic Gene Regulatory Program
Authors: Hofmann Bowman Marion A. , Gawdzik Joseph , Bukhari Usama , Husain Aliya N. , Toth Peter T. , Kim Gene , Earley Judy , McNally Elizabeth M. 
Published: October 2010

Objective—
The proinflammatory cytokine S100A12 is associated with coronary atherosclerotic plaque rupture. We previously generated transgenic mice with vascular smooth muscle–targeted expression of human S100A12 and found that these mice developed aortic aneurysmal dilation of the thoracic aorta. In the current study, we tested the hypothesis that S100A12 expressed in vascular smooth muscle in atherosclerosis-prone apolipoprotein E (ApoE)–null mice would accelerate atherosclerosis.

Methods and Results—
ApoE-null mice with or without the S100A12 transgene were analyzed. We found a 1.4-fold increase in atherosclerotic plaque size and more specifically a large increase in calcified plaque area (45% versus 7% of innominate artery plaques and 18% versus 10% of aortic root plaques) in S100A12/ApoE-null mice compared with wild-type/ApoE-null littermates. Expression of bone morphogenic protein and other osteoblastic genes was increased in aorta and cultured vascular smooth muscle, and importantly, these changes in gene expression preceded the development of vascular calcification in S100A12/ApoE-null mice. Accelerated atherosclerosis and vascular calcification were mediated, at least in part, by oxidative stress because inhibition of NADPH oxidase attenuated S100A12-mediated osteogenesis in cultured vascular smooth muscle cells. S100A12 transgenic mice in the wild-type background (ApoE+/+) showed minimal vascular calcification, suggesting that S100A12 requires a proinflammatory/proatherosclerotic environment to induce osteoblastic differentiation and vascular calcification.

Conclusion—
Vascular smooth muscle S100A12 accelerates atherosclerosis and augments atherosclerosis-triggered osteogenesis, reminiscent of features associated with plaque instability.
Maturational Constraints on the Recruitment of Early Processes for Syntactic Processing
Authors: Pakulak Eric , Neville Helen J. 
Published: October 2010

AbstractAn enduring question in the study of second-language acquisition concerns the relative contributions of age of acquisition (AOA) and ultimate linguistic proficiency to neural organization for second-language processing. Several ERP and neuroimaging studies of second-language learners have found that neural organization for syntactic processing is sensitive to delays in second-language acquisition. However, such delays in second-language acquisition are typically associated with lower language proficiency, rendering it difficult to assess whether differences in AOA or proficiency lead to these effects. Here we examined the effects of delayed second-language acquisition while controlling for proficiency differences by examining participants who differ in AOA but who were matched for proficiency in the same language. We compared the ERP response to auditory English phrase structure violations in a group of late learners of English matched for grammatical proficiency with a group of English native speakers. In the native speaker group, violations elicited a bilateral and prolonged anterior negativity, with onset at 100 msec, followed by a posterior positivity (P600). In contrast, in the nonnative speaker group, violations did not elicit the early anterior negativity, but did elicit a P600 which was more widespread spatially and temporally than that of the native speaker group. These results suggest that neural organization for syntactic processing is sensitive to delays in language acquisition independently of proficiency level. More specifically, they suggest that both early and later syntactic processes are sensitive to maturational constraints. These results also suggest that late learners who reach a high level of second-language proficiency rely on different neural mechanisms than native speakers of that language.
White Matter Microstructure in Superior Longitudinal Fasciculus Associated with Spatial Working Memory Performance in Children
Authors: Vestergaard Martin , Madsen Kathrine Skak , Baaré William F. C. , Skimminge Arnold , Ejersbo Lisser Rye , Ramsøy Thomas Z. , Gerlach Christian , Åkeson Per , Paulson Olaf B. , Jernigan Terry L. 
Published: October 2010

AbstractDuring childhood and adolescence, ongoing white matter maturation in the fronto-parietal cortices and connecting fiber tracts is measurable with diffusion-weighted imaging. Important questions remain, however, about the links between these changes and developing cognitive functions. Spatial working memory (SWM) performance improves significantly throughout the childhood years, and several lines of evidence implicate the left fronto-parietal cortices and connecting fiber tracts in SWM processing. Here we report results from a study of 76 typically developing children, 7 to 13 years of age. We hypothesized that better SWM performance would be associated with increased fractional anisotropy (FA) in a left fronto-parietal network composed of the superior longitudinal fasciculus (SLF), the regional white matter underlying the dorsolateral pFC, and the posterior parietal cortex. As hypothesized, we observed a significant association between higher FA in the left fronto-parietal network and better SWM skills, and the effect was independent of age. This association was mainly accounted for by variability in left SLF FA and remained significant when FA measures from global fiber tracts or right SLF were included in the model. Further, the effect of FA in left SLF appeared to be mediated primarily by decreasing perpendicular diffusivity. Such associations could be related to individual differences among children in the architecture of fronto-parietal connections and/or to differences in the pace of fiber tract development. Further studies are needed to determine the contributions of intrinsic and experiential factors to the development of functionally significant individual differences in fiber tract structure.
Ivan the Terrible and His Women
Authors: Keenan Edward 
Published: October 2010

AbstractThis article presents the text of two lectures delivered by the author in the Kathryn W. Davis Lecture Series at Wellesley College on January 31 and February 21, 1980. In the first lecture, the author describes the “grammar” of the Muscovite political system, which was established after the Muscovite dynasty's victory over its rivals (and relatives) in the civil wars of the mid-fifteenth century and which established succession by primogeniture in the ruling house. That system was, according to the author, founded on a collaborative power arrangement between the grand prince and his boyars, and was united and reinforced by a web of interconnecting marriages over several generations, the most important marriage in each generation being the ruler's. Politics in this system was marriage politics, and the product of it was an oligarchical political system built on kinship and consensus. In the second lecture, the author focuses on the role women played in Muscovite political culture. Here the emphasis is on both elite women—the wives, mothers, sisters, and aunts of the rulers—and the nannies, drawn usually from humbler backgrounds, who served in the Kremlin and introduced to those living there many of the conventions of language and literature that remain a foundation of modern Russian. These lectures offer a synthetic approach to the political culture of Muscovy, uniting an innovative anthropological perspective on court politics with a pathbreaking analysis of the lasting impact women had on the culture—political, social, biological, and linguistic—in the Kremlin.
“For the Firm Maintenance of the Dignity and Tranquility of the Imperial Family”: Law and Familial Order in the Romanov Dynasty
Authors: Martin Russell 
Published: October 2010

AbstractThis article examines the Law of Succession and the Statute on the Imperial Family, texts which were issued by Emperor Paul I on April 5, 1797, and which regulated the succession to the throne and the structure of the Romanov dynasty as a family down to the end of the empire in 1917. It also analyzes the revisions introduced in these texts by subsequent emperors, focusing particularly on the development of a requirement for equal marriage and for marrying Orthodox spouses. The article argues that changing circumstances in the dynasty—its rapid increase in numbers, its growing demands on the financial resources of the Imperial Household, its struggle to resist morganatic and interfaith marriages—forced changes to provisions in the Law and Statute, and that the Romanovs debated and reformed the structure of the Imperial Family in the context of the provisions of these laws. The article shows how these Imperial House laws served as a vitally important arena for reform and legal culture in pre-revolutionary Russia.
The Emergence of Ethnic Politics in 1905: The League for the Attainment of Full Rights for the Jews of Russia
Authors: Orbach Alexander 
Published: October 2010

AbstractThe legalization of political activity in Tsarist Russia in 1905 created the opportunity for Jewish liberals to enter the public arena as proponents of civic and political rights for the Jews of the realm. However, unlike Jewish liberals in western and central Europe, Russian-Jewish liberals also called for the extension of national cultural rights for the Jews of Russia in addition to that of individual emancipation. They formed the League for the Attainment of Full Rights for the Jews of Russia in March 1905 and proceeded to prepare for the upcoming elections for the proposed assembly to be elected by the people later that year. However, the violent pogroms that broke out in the fall of 1905 generated a more nationalist minded-agenda as these liberals found themselves in competition with both Jewish nationalists and Jewish socialists for the attention of the people.
Green Roof Mitigation Potential for a Proxy Future Climate Scenario in Chicago, Illinois
Authors: Smith Kathryn R. , Roebber Paul J. 
Published: October 2010

Abstract
The Advanced Research version of the Weather Research and Forecasting Model (ARW) coupled with an urban canopy model is used to investigate the potential of vegetative (green) roof technology to mitigate against ongoing climate warming and continued urban sprawl for a day representing average summer conditions in late-twenty-first-century Chicago, Illinois. Effects related particularly to human health hazards resulting from excessive heat and high pollution concentrations are emphasized. Continued expansion of the urban environment over the next century is shown to lead to an expansion of the warming signal across the metropolitan region. Widespread adoption of vegetative rooftops, through increased albedo and evapotranspiration, reduces temperatures in the urban environment by as much as 3°C, an effect similar to the simpler but less appealing alternative of employing painted or other reflective rooftop structures (e.g., white roofs). A significant limitation to the green roof approach for the case studied is that the increase in moisture resulting from transpiration leads to only marginal cooling when apparent temperatures are considered. An additional complication arises in that the reduced temperatures alter the lake-breeze circulation, potentially reducing circulation of pollutants into the city core, but also reducing natural cooling in the most urbanized areas during the climatologically warmest hours. Future work that evaluates these impacts over a broader range of synoptic settings, documents changes in the planetary boundary layer structure and attendant pollution, and considers the multiple-day dependence of these effects is needed.