bluuebunny/crossref_metadata_2025_split huggingface dataset:
Exploration of poly (ADP‐ribose) polymerase inhibitor resistance in the treatment of BRCA1/2‐mutated cancer
Authors: Wu Shuyi , Yao Xuanjie , Sun Weiwei , Jiang Kaitao , Hao Jie 
Published: May 2024

AbstractBreast cancer susceptibility 1/2 (BRCA1/2) genes play a crucial role in DNA damage repair, yet mutations in these genes increase the susceptibility to tumorigenesis. Exploiting the synthetic lethality mechanism between BRCA1/2 mutations and poly(ADP‐ribose) polymerase (PARP) inhibition has led to the development and clinical approval of PARP inhibitor (PARPi), representing a milestone in targeted therapy for BRCA1/2 mutant tumors. This approach has paved the way for leveraging synthetic lethality in tumor treatment strategies. Despite the initial success of PARPis, resistance to these agents diminishes their efficacy in BRCA1/2‐mutant tumors. Investigations into PARPi resistance have identified replication fork stability and homologous recombination repair as key factors sensitive to PARPis. Additionally, studies suggest that replication gaps may also confer sensitivity to PARPis. Moreover, emerging evidence indicates a correlation between PARPi resistance and cisplatin resistance, suggesting a potential overlap in the mechanisms underlying resistance to both agents. Given these findings, it is imperative to explore the interplay between replication gaps and PARPi resistance, particularly in the context of platinum resistance. Understanding the impact of replication gaps on PARPi resistance may offer insights into novel therapeutic strategies to overcome resistance mechanisms and enhance the efficacy of targeted therapies in BRCA1/2‐mutant tumors.
Uncovering the WWTR1::NCOA2 Gene fusion in low‐grade myoepithelial‐rich neoplasm with HMGA2 expression: A case report
Authors: Alsugair Ziyad , Pissaloux Daniel , Descotes Françoise , Tirode Franck , Lopez Jonathan , Perrot Jimmy , Lapierre Ariane , Fieux Maxime , Philouze Pierre , Champagnac Anne , Onea Mihaela , Benzerdjeb Nazim 
Published: May 2024

AbstractWe describe a case of a pleomorphic adenoma (PA) arising from the para‐tracheal accessory salivary gland in a 44‐year‐old male harboring a novel WWTR1::NCOA2 gene fusion. To our knowledge, this novel gene fusion has not been described previously in salivary gland tumors. The patient presented with hoarseness of voice. The radiological exam revealed a mass in the upper third of the trachea involving the larynx. Histologically, the tumor consisted of bland‐looking monocellular eosinophilic epithelial cells arranged in cords and sheets separated by thin fibrous stroma, focally forming a pseudo‐tubular pattern. In immunohistochemistry, the tumor cells demonstrated positivity for CK7, PS100, SOX10, and HMGA2; and negativity for CK5/6, p40 p63, and PLAG1. In addition, the clustering analysis clearly demonstrates a clustering of tumors within the PA group. In addition to reporting this novel fusion in the PA spectrum, we discuss the relevant differential diagnoses and briefly review of NCOA2 and WWTR1 gene functions in normal and neoplastic contexts.
Individual differences in learning agility at work: A mixed methods study to develop and validate a new scale
Authors: Milani Roberta , Sommovigo Valentina , Ghirotto Luca , Setti Ilaria 
Published: May 2024

AbstractThe unprecedented complexity of today's business and working environment increases the need for leaders and employees to learn new skills, be adaptable, and open to embracing change. New abilities and mindsets emerge, and their evaluation is challenging. This research utilized an exploratory sequential mixed methods design to develop and provide a preliminary validation of a scale measuring individual differences in learning agility (LA) at work. The measure has been tested in Central‐Eastern European countries, Italy, and Egypt. Results showed satisfactory reliability and nomological validity. The final scale of 11 items provides HRD researchers and practitioners with a reliable and concise tool suitable for investigating individual differences in LA across different job roles and positions. The scale has been tested in a cross‐cultural setting and is available in English, making it especially appropriate for multinational contexts.
Study of the relationship between psychoactive substance use and pain in cancer patients and cancer survivors: A French nationwide cross‐sectional study
Authors: Ragusa Charles , Pereira Bruno , Balayssac David 
Published: May 2024

AbstractPain is a prevalent symptom among cancer patients and survivors. Psychoactive substance use (PSU) is associated with both the presence and severity of pain. However, little is known about this association in the context of cancer. The primary objective was to compare the prevalence of PSU and its relationship with pain during and after cancer. PSU was defined as the use of nonmedication substances (alcohol, tobacco, e‐cigarettes, cannabidiol, and cannabis), with frequency categorized as at least yearly, monthly, weekly, or daily. Secondary objectives aimed to explore the relationships between PSU and pain characteristics, health‐related quality of life, anxiety, depression, deprivation, and individual characteristics. Among the 1041 individuals included, pain prevalence was 44.7% (95% confidence interval [CI] 41.6%–47.8%). The overall prevalence of PSU at least monthly was 67.0% (95% CI 64.0%–69.8%). The proportions of chronic and neuropathic pains were higher for at least monthly use of cannabidiol compared to nonuse (70.0% vs. 39.3% and 55.7% vs. 28.1%, p < .001). In multivariate analysis, the monthly uses of tobacco and cannabidiol were higher in painful individuals than in nonpainful ones (odds ratio: 2.85 [95% CI 1.22–6.64] and 3.76 [95% CI 1.13–12.44], p < .05). From the point of view of the patient care, the study underscores the need for physicians to prioritize smoking cessation and pay attention to the use of cannabidiol during and after cancer.
Impact of a feedback strategy in a series of communication‐focused patient care simulations
Authors: Rao Hindu , Beuttler Richard , Dintzner Madeline , Taheri Reza , Bach Albert T. , Fakourfar Neeloufar 
Published: May 2024

AbstractIntroductionPatient care simulations (PCS) and objective structured clinical examinations (OSCE) allow pharmacy students to practice communication. Feedback can help improve communication, but the impact over time is not well understood.ObjectiveThis study investigated the impact of a feedback strategy on pharmacy students' communication skills over three PCS. It also evaluated the alignment between students' self‐scoring and faculty scoring.MethodsPharmacy students participated in three sessions (PCS1, OSCE, and PCS3) that were focused on the affective domain. Individualized numerical and narrative feedback was provided to students on their performance after PCS1. Students' communication was scored by faculty graders out of an 18‐point validated rubric. Students self‐scored their communication with the same rubric. Faculty and student scores were compared using a linear mixed effects model, and an intraclass correlation coefficient was used to measure agreement.ResultsIn PCS1, 82 students scored an average of 15.41 ± 2.14 for faculty scores and 16.06 ± 1.55 for self‐graded scores (0.36, p < 0.001). In the OSCE, 81 students had an average of 15.93 ± 1.86 for faculty scores and 16.45 ± 1.35 for self‐graded scores (0.1, p = 0.18). In PCS3, 74 students scored an average of 15.22 ± 2.15 for faculty scores and 16.25 ± 1.44 for self‐graded scores (0.14, p = 0.08). A correlation between faculty and student scores was seen for PCS1. Over the three sessions, no significant differences were found between student self‐graded scores (p = 0.08), but faculty scores did differ, with the OSCE having higher scores than PCS3 (p < 0.01). Many students with faculty‐graded scores greater than 1 standard deviation below the mean scored themselves higher than faculty did.ConclusionFeedback after PCS1 did not significantly improve scores. Students with low faculty‐graded scores frequently scored themselves higher indicating low self‐awareness.
Emotions in engineering education: A configurative meta‐synthesis systematic review
Authors: Lönngren Johanna , Bellocchi Alberto , Berge Maria , Bøgelund Pia , Direito Inês , Huff James L. , Mohd‐Yusof Khairiyah , Murzi Homero , Farahwahidah Abdul Rahman Nor , Tormey Roland 
Published: May 2024

AbstractBackgroundThe study of emotions in engineering education (EEE) has increased in recent years, but this emerging, multidisciplinary body of research is dispersed and not well consolidated. This paper reports on the first systematic review of EEE research and scholarship.PurposeThe review aimed to critically assess how researchers and scholars in engineering education have conceptualized emotions and how those conceptualizations have been used to frame and conduct EEE research and scholarship.Scope/MethodThe systematic review followed the procedures of a configurative meta‐synthesis, mapping emotion theories and concepts, research purposes and methods, and citation patterns in the EEE literature. The review proceeded through five stages: (i) scoping and database searching; (ii) abstract screening, full text sifting, and full text review; (iii) pearling; (iv) scoping review, and (v) in‐depth analysis for the meta‐synthesis review. Two hundred and thirteen publications were included in the final analysis.ResultsThe results show that the EEE literature has not extensively engaged with the wide range of conceptualizations of emotion available in the educational, psychological, and sociological literature. Further, the focus on emotion often seems to have been unintentional and of secondary importance in studies whose primary goals were to study other phenomena.ConclusionsMore research adopting intentional, theorized approaches to emotions will be crucial in further developing the field. To do justice to complex emotional phenomena in teaching and learning, future EEE research will also need to engage a broader range of conceptualizations of emotion and research methods, drawing on diverse disciplinary traditions.
Sevoflurane blocks KLF5‐mediated transcriptional activation of ITGB2 to inhibit macrophage infiltration in hepatic ischemia/reperfusion injury
Authors: Li Ye , Gao Weinian , Lei Shuyan , Wu Xiaoning , Yuan Tao , Ma Kai , Chi Kui 
Published: May 2024

AbstractBackgroundSevoflurane (Sevo) preconditioning and postconditioning play a protective role against injury induced by hepatic ischemia/reperfusion (I/R). At the same time, the involvement of macrophage infiltration in this process and the precise mechanisms are unclear. Here, we designed this research to elucidate the protective effects of Sevo against hepatic I/R injury and the molecules involved.MethodsThe alleviating effect of Sevo on the liver injury was analyzed by liver function analysis, hematoxylin and eosin staining, Masson trichrome staining, terminal deoxynucleotidyl transferase‐mediated 2′‐deoxyuridine 5′‐triphosphate nick end labeling, western blot analysis and an enzyme‐linked immunosorbent assay. An in vitro cell model was developed using alpha mouse liver 12 (AML12) cells, and the cell model was treated with oxygen–glucose deprivation and reoxygenation and Sevo. Multiple bioinformatics databases were used to screen transcriptional regulators related to hepatic I/R injury and the targets of Krueppel‐like factor 5 (KLF5). KLF5 expression was artificially upregulated alone or with integrin beta‐2 (ITGB2) knockdown to substantiate their involvement in Sevo‐mediated hepatoprotection.ResultsSevo protected the liver against I/R injury by reducing cell apoptosis and inflammatory response. KLF5 was upregulated in liver tissues following I/R injury, whereas KLF5 overexpression aggravated macrophage infiltration and liver injury induced by I/R injury. KLF5 bound to the promoter of ITGB2 to enhance ITGB2 transcription. Knockdown of ITGB2 reversed the aggravation of injury caused by KLF5 overexpression in mice and AML12 cells.ConclusionsSevo blocked KLF5‐mediated transcriptional activation of ITGB2, thereby inhibiting macrophage infiltration in hepatic I/R injury.
Stable engraftment, as well as graft versus host disease‐free and relapse‐free survival brought by the combination of CD7 targeted universal chimeric antigen receptor‐T, and donor hemopoietic stem cells: Indication of a case report
Authors: Li Shiqi , Yuan Zhongtao , Liu Lin , Li Yu , Luo Le , Peng Lihui , Xu Mengli , Yin Ping , Chen Yingnian , Li Xiaoping , Zang Qingying , Zhang Xi , Wang Sanbin 
Published: May 2024

AbstractCD7 targeted CAR‐T has demonstrated potential in the treatment of T cell malignancies but no study has been reported about its potential in the prophylaxis of GVHD in allo‐HSCT. Here we reported a special case that a boy diagnosed with refractory acute T lymphoblastic leukemia (T‐ALL) was treated with universal CD7 targeted CAR‐T (CD7 UCAR‐T) and parent‐derived peripheral blood stem cells (PBSCs). Complete remission and full engraftment of donor was observed. In the later four months of follow‐up, in the absence of any immunodepression treatment, no signs of GVHD were observed. This case initially demonstrates the potential of CD7 UCAR‐T in the prophylaxis of GVHD.
Improving Routine 89Zr‐Immuno‐PET Applications: Mild Iron Removal Can Favor the Use of Fe‐DFO‐N‐suc‐TFP Ester Over p‐NCS‐Bz‐DFO
Authors: Wuensche Thomas E. , Nauta Sabine , van Dongen Guus A. M. S. , Vugts Danielle J. 
Published: May 2024

ABSTRACTA key aspect for the applicability of 89Zr‐radioimmunoconjugates is inert modification and radiolabeling. The two commercially available bifunctional variants of the siderophore desferrioxamine (DFO), Fe‐DFO‐N‐suc‐TFP‐ester and p‐NCS‐Bz‐DFO, are most often used for clinical 89Zr‐immuno‐PET. The use of Fe‐DFO‐N‐suc‐TFP‐ester is advantageous with regard to higher radiolysis stability and more facile assessment of radiochemical purity as well as chelator‐to‐mAb ratio. However, not all mAbs withstand the Fe‐removal step at relatively low pH (4–4.5) using EDTA, which is needed after conjugation to allow 89Zr labeling. In this study, it was investigated whether hydroxybenzyl ethylenediamine (HBED) or the clinically approved deferiprone (DFP) can serve as an alternative for EDTA to establish a pH‐independent mild method for Fe‐removal and thereby broaden the applicability of Fe‐DFO‐N‐suc‐TFP‐ester. Carrier‐added [59Fe]Fe‐DFO‐N‐suc‐TFP‐ester was used for mAb modification to enable direct tracking of the Fe‐removal efficiency under various conditions. Whereas incomplete Fe‐removal with HBED was observed at pH 5 or higher, Fe‐removal with DFP was possible at a broad pH range (4–9). This provides a mild, pH‐independent method for Fe‐removal, improving the applicability and attractiveness of Fe‐DFO‐N‐suc‐TFP‐ester for 89Zr‐mAb preparation.
Presence of SARS‐CoV‐2 RNA in COVID‐19 survivors with post‐COVID symptoms 2 years after hospitalization: The VIPER study
Authors: Fernández‐de‐las‐Peñas César , Torres‐Macho Juan , Ruiz‐Ruigómez Maria , Arrieta‐Ortubay Estibaliz , Rodríguez‐Rebollo Carolina , Akasbi‐Moltalvo Míriam , Pardo‐Guimerá Virginia , Ryan‐Murua Pablo , Lumbreras‐Bermejo Carlos , Pellicer‐Valero Oscar J. , Giordano Rocco , Arendt‐Nielsen Lars , Franco‐Moreno Anabel 
Published: May 2024

AbstractThe SARS‐CoV‐2 VIrus PERsistence (VIPER) study investigated the presence of long‐lasting SARS‐CoV‐2 RNA in plasma, stool, urine, and nasopharyngeal samples in COVID‐19 survivors. The presence of SARS‐CoV‐2 RNA reverse transcription polymerase chain reactions (RT‐PCR) were analyzed within plasma, stool, urine, and nasopharyngeal swab samples in COVID‐19 survivors with post‐COVID symptoms and a comparison group of COVID‐19 survivors without post‐COVID symptoms matched by age, sex, body mass index and vaccination status. Participants self‐reported the presence of any post‐COVID symptom (defined as a symptom that started no later than 3 months after the initial infection). Fifty‐seven (57.9% women, age: 51.1, standard deviation [SD]: 10.4 years) previously hospitalized COVID‐19 survivors with post‐COVID symptoms and 55 (56.4% women, age: 50.0, SD: 12.8 years) matched individuals who had a past SARS‐CoV‐2 infection without post‐COVID symptoms were evaluated 27 (SD 7.5) and 26 (SD 8.7) months after hospital discharge, respectively. The presence of SARS‐CoV‐2 RNA was identified in three nasopharyngeal samples of patients with post‐COVID symptoms (5.2%) but not in plasma, stool, or urine samples. Thus, SARS‐CoV‐2 RNA was not identified in any sample of survivors without post‐COVID symptoms. The most prevalent post‐COVID symptoms consisted of fatigue (93%), dyspnea, and pain (both, 87.7%). This study did not find SARS‐CoV‐2 RNA in plasma, stool, or urine samples, 2 years after the infection. A prevalence of 5.2% of SARS‐CoV‐2 RNA in nasopharyngeal samples, suggesting a potential active or recent reinfection, was found in patients with post‐COVID symptoms. These results do not support the association between SARS‐CoV‐2 RNA in plasma, stool, urine, or nasopharyngeal swab samples and post‐COVID symptomatology in the recruited population.
Aerosol impacts on the East Asian winter monsoon: Insights from TaiESM1 and CMIP6 simulations
Authors: Tsai I‐Chun , Yang Szu‐Wei , Shiu Chein‐Jung , Chen Yi‐Ying , Chen Cheng‐An , Lee Wei‐Liang , Hsu Huang‐Hsiung 
Published: May 2024

AbstractThe East Asian winter monsoon (EAWM) is an important climate pattern in Southeast Asia. This study delved into the aerosol effects on the EAWM by analysing simulations conducted with the Taiwan Earth System Model version 1 (TaiESM1) model, part of the Coupled Model Intercomparison Project Phase 6 (CMIP6). Our assessment, validated against observational and reanalysis data, revealed that TaiESM1 effectively replicates the robust circulation of the EAWM and aerosol concentrations, placing its performance above average level among CMIP6 models. Notably, the simulated global mean aerosol optical thickness exhibited an approximately 20% increase from 1850 to 2014. The increased aerosols corresponded to a weakened Aleutian Low and an intensified Siberian High, leading to a weakened EAWM in the lower atmosphere over extratropical areas. In addition, the cooling across the Northern Hemisphere might affect enhanced northerly winds at approximately 10°N, which are situated in the southern part of the EAWM. An analysis of the near‐surface temperature budget indicated that the aerosol‐induced cooling in East China and India was primarily driven by changes in shortwave and longwave radiation fluxes, albeit slightly offset by sensible heat and latent heat fluxes. Furthermore, our study also reveals that while the mechanisms through which anthropogenic aerosols affect the northern portion of the EAWM vary across most CMIP6 models, their impacts on the tropical region remain consistent.
Necessity entrepreneurship: A journey from unemployment to self‐employment
Authors: Lim Weng Marc , Bansal Sanchita , Kumar Satish , Singh Shifali , Nangia Priya 
Published: May 2024

AbstractThis study provides a comprehensive examination of necessity entrepreneurship, a concept distinct from its counterpart, opportunity entrepreneurship. Necessity entrepreneurship emerges from the compelling need to address economic hardship or unemployment, contrasting with opportunity entrepreneurship, which is driven by the pursuit of new market opportunities. The backdrop of this study is anchored in the escalating unemployment crisis and its implications for sustainable economic growth and the attainment of sustainable development goals. Through an integrative review combining bibliometric–content analysis of 96 publications, following the PRISMA protocol, and a detailed case analysis of 10 globally recognized entrepreneurs who embarked on their ventures driven by necessity, this study unravels the critical factors fueling the rise of necessity entrepreneurship. The findings delineate the relationship between necessity and opportunity entrepreneurship, highlighting how an entrepreneurial orientation can serve as a pivotal link. This orientation not only mitigates the adverse effects of unemployment but also propels the economy toward sustainable growth, thereby contributing significantly to the sustainable development goals. The insights derived from this study traverse multiple domains including entrepreneurial orientation, socio‐economic factors, as well as innovation and digitalization, offering a granular perspective on business policy, entrepreneurial ecosystems, the disparate impacts on developed versus developing nations, and the role of innovation in dynamic entrepreneurship. Through a conceptual framework, this study elucidates how entrepreneurial orientation can effectively bridge the gap between the unemployment crisis and sustainable economic growth. Therefore, this study not only enriches the academic discourse on necessity entrepreneurship but also provides pragmatic insights for entrepreneurs, policymakers, and scholars, emphasizing the crucial role of entrepreneurship in navigating economic and social challenges.
Radiomic features of the medial meniscus predicts incident destabilizing meniscal tears: Data from the osteoarthritis initiative
Authors: Villagran Michelle , Driban Jeffrey B. , Lu Bing , MacKay James W. , McAlindon Timothy E. , Harkey Matthew S. 
Published: May 2024

AbstractThe objective of this study was to determine the optimal meniscal radiomic features to classify people who will develop an incident destabilizing medial meniscal tear. We used magnetic resonance (MR) images from an existing case–control study that includes images from the first 4 years of the Osteoarthritis Initiative (OAI). For this exploratory analysis (n = 215), we limited our study sample to people with (1) intact menisci at the OAI baseline visit, (2) 4‐year meniscal status data, and (3) complete meniscal data from each region of interest. Incident destabilizing meniscal tear was defined as progressing from an intact meniscus to a destabilizing tear by the 48‐month visit using intermediate‐weighted fat‐suppressed MR images. One reader manually segmented each participant's anterior and posterior horn of the medial menisci at the OAI baseline visit. Next, 61 different radiomic features were extracted from each medial meniscus horn. We performed a classification and regression tree (CART) analysis to determine the classification rules and important variables that predict incident destabilizing meniscal tear. The CART correctly classified 24 of the 34 cases and 172 out of 181 controls with a sensitivity of 70.6% and a specificity of 95.0%. The CART identified large zone high gray level emphasis (i.e., more coarse texture) from the posterior horn as the most important variable to classify who would develop an incident destabilizing medial meniscal tear. The use of radiomic features provides sensitive and quantitative measures of meniscal alterations, allowing us to intervene and prevent destabilizing meniscal tears.