bluuebunny/crossref_metadata_2025_split huggingface dataset:
CAPE in Tropical Cyclones
Authors: Molinari John , Romps David M. , Vollaro David , Nguyen Leon 
Published: March 2012

Abstract
Convective available potential energy (CAPE) and the vertical distribution of buoyancy were calculated for more than 2000 dropsonde soundings collected by the NOAA Gulfstream-IV aircraft. Calculations were done with and without the effects of condensate loading, entrainment, and the latent heat of fusion. CAPE showed larger values downshear than upshear within 400 km of the center, consistent with the observed variation of convective intensity. The larger downshear CAPE arose from (i) higher surface specific humidity, (ii) lower midtropospheric temperature, and, for entraining CAPE, (iii) larger free-tropospheric relative humidity.
Reversible CAPE had only one-half the magnitude of pseudoadiabatic CAPE. As shown previously, reversible CAPE with fusion closely resembled pseudoadiabatic CAPE without fusion. Entrainment had the most dramatic impact. Entraining CAPE was consistent with the observed radial distribution of convective intensity, displaying the largest values downshear at inner radii. Without entrainment, downshear CAPE was smallest in the core and increased outward to the 600-km radius.
The large number of sondes allowed the examination of soundings at the 90th percentile of conditional instability, which reflect the conditions leading to the most vigorous updrafts. Observations of convection in tropical cyclones prescribe the correct method for calculating this conditional instability. In particular, the abundance and distribution of vigorous deep convection is most accurately reflected by calculating CAPE with condensate retention and a fractional entrainment rate in the range of 5%–10% km−1.
Acatalasemic mice are mildly susceptible to adriamycin nephropathy and exhibit increased albuminuria and glomerulosclerosis
Authors: Takiue Keiichi , Sugiyama Hitoshi , Inoue Tatsuyuki , Morinaga Hiroshi , Kikumoto Yoko , Kitagawa Masashi , Kitamura Shinji , Maeshima Yohei , Wang Da-Hong , Masuoka Noriyoshi , Ogino Keiki , Makino Hirofumi 
Published: March 2012

Abstract

Background
Catalase is an important antioxidant enzyme that regulates the level of intracellular hydrogen peroxide and hydroxyl radicals. The effects of catalase deficiency on albuminuria and progressive glomerulosclerosis have not yet been fully elucidated. The adriamycin (ADR) nephropathy model is considered to be an experimental model of focal segmental glomerulosclerosis. A functional catalase deficiency was hypothesized to exacerbate albuminuria and the progression of glomerulosclerosis in this model.

Methods
ADR was intravenously administered to both homozygous acatalasemic mutant mice (C3H/AnLCsbCsb) and control wild-type mice (C3H/AnLCsaCsa). The functional and morphological alterations of the kidneys, including albuminuria, renal function, podocytic, glomerular and tubulointerstitial injuries, and the activities of catalase were then compared between the two groups up to 8 weeks after disease induction. Moreover, the presence of a mutation of the toll-like receptor 4 (tlr4) gene, which was previously reported in the C3H/HeJ strain, was investigated in both groups.

Results
The ADR-treated mice developed significant albuminuria and glomerulosclerosis, and the degree of these conditions in the ADR-treated acatalasemic mice was higher than that in the wild-type mice. ADR induced progressive renal fibrosis, renal atrophy and lipid peroxide accumulation only in the acatalasemic mice. In addition, the level of catalase activity was significantly lower in the kidneys of the acatalasemic mice than in the wild-type mice during the experimental period. The catalase activity increased after ADR injection in wild-type mice, but the acatalasemic mice did not have the ability to increase their catalase activity under oxidative stress. The C3H/AnL strain was found to be negative for the tlr4 gene mutation.

Conclusions
These data indicate that catalase deficiency plays an important role in the progression of renal injury in the ADR nephropathy model.
The tumour metabolism inhibitors GSAO and PENAO react with cysteines 57 and 257 of mitochondrial adenine nucleotide translocase
Authors: Park Danielle , Chiu Joyce , Perrone Gabriel G , Dilda Pierre J , Hogg Philip J 
Published: March 2012

Abstract

Background
GSAO (4-(N-(S-glutathionylacetyl)amino) phenylarsonous acid) and PENAO (4-(N-(S-penicillaminylacetyl)amino) phenylarsonous acid) are tumour metabolism inhibitors that target adenine nucleotide translocase (ANT) of the inner-mitochondrial membrane. Both compounds are currently being trialled in patients with solid tumours. The trivalent arsenical moiety of GSAO and PENAO reacts with two matrix facing cysteine residues of ANT, inactivating the transporter. This leads to proliferation arrest and death of tumour and tumour-supporting cells.

Results
The two reactive ANT cysteine residues have been identified in this study by expressing cysteine mutants of human ANT1 in Saccharomyces cerevisiae and measuring interaction with the arsenical moiety of GSAO and PENAO. The arsenic atom of both compounds cross-links cysteine residues 57 and 257 of human ANT1.

Conclusions
The sulphur atoms of these two cysteines are 20 Å apart in the crystal structures of ANT and the optimal spacing of cysteine thiolates for reaction with As (III) is 3-4 Å. This implies that a significant conformational change in ANT is required for the organoarsenicals to react with cysteines 57 and 257. This conformational change may relate to the selectivity of the compounds for proliferating cells.
RhoA: A therapeutic target for chronic myeloid leukemia
Authors: Molli Poonam R , Pradhan Madhura B , Advani Suresh H , Naik Nishigandha R 
Published: March 2012

Abstract

Background
Chronic Myeloid Leukemia (CML) is a malignant pluripotent stem cells disorder of myeloid cells. In CML patients, polymorphonuclear leukocytes (PMNL) the terminally differentiated cells of myeloid series exhibit defects in several actin dependent functions such as adhesion, motility, chemotaxis, agglutination, phagocytosis and microbicidal activities. A definite and global abnormality was observed in stimulation of actin polymerization in CML PMNL. Signalling molecules ras and rhoGTPases regulate spatial and temporal polymerization of actin and thus, a broad range of physiological processes. Therefore, status of these GTPases as well as actin was studied in resting and fMLP stimulated normal and CML PMNL.

Methods
To study expression of GTPases and actin, Western blotting and flow cytometry analysis were done, while spatial expression and colocalization of these proteins were studied by using laser confocal microscopy. To study effect of inhibitors on cell proliferation CCK-8 assay was done. Significance of differences in expression of proteins within the samples and between normal and CML was tested by using Wilcoxon signed rank test and Mann-Whitney test, respectively. Bivariate and partial correlation analyses were done to study relationship between all the parameters.

Results
In CML PMNL, actin expression and its architecture were altered and stimulation of actin polymerization was absent. Differences were also observed in expression, organization or stimulation of all the three GTPases in normal and CML PMNL. In normal PMNL, ras was the critical GTPase regulating expression of rhoGTPases and actin and actin polymerization. But in CML PMNL, rhoA took a central place. In accordance with these, treatment with rho/ROCK pathway inhibitors resulted in specific growth inhibition of CML cell lines.

Conclusions
RhoA has emerged as the key molecule responsible for functional defects in CML PMNL and therefore can be used as a therapeutic target in CML.
Harmful effects of carbamazepine on the postnatal development of the rat ventral prostate
Authors: Oliva Samara U , Scarano Wellerson R , Okada Fatima K , Miraglia Sandra M 
Published: March 2012

Abstract

Background
Carbamazepine (CBZ) is a first-line antiepileptic drug (AED), although it is also used for the treatments of psychiatric disorders and neuropathic pain. The CBZ utilization has been associated with male reproductive damage, including hormonal alterations, sexual dysfunction and reduction of sperm quality. The wide and long-term use of the CBZ is a common schedule in children and adolescents and alters the testosterone level in adult rats and humans. The objective of this work was to evaluate the CBZ side effects on the ventral prostate of rats from pre-puberty to sexual maturation, since the prostate is an androgen-dependent organ.

Methods
Twenty three day-old male albino Wistar rats received CBZ diluted in propylene glycol (20 mg/Kg/i.p via). The treatment lasted 20, 40 and 70 days, according to the different stages of the rat sexual maturation. At the end of each treatment period, ventral prostates were removed and histologically processed. The prostate sections were submitted to the histopathological, morphological and stereological analyses using image analysis system.

Results
Reductions of the glandular epithelium, glandular lumen and fibromuscular stroma volume of the ventral prostate were observed in adult rats treated with CBZ since the weaning. Triggering and degranulation of mast cells were observed in the fibromuscular stroma of prepubertal and pubertal CBZ treated rats.

Conclusions
The results suggest a direct effect of the CBZ on rat ventral prostate, evidenced by increase of mast cell and macrophage populations during pre-puberty and puberty causing a ventral prostate accentuated damage in the adult phase.
Acute Morphine Induces Matrix Metalloproteinase-9 Up-Regulation in Primary Sensory Neurons to Mask Opioid-Induced Analgesia in Mice
Authors: Liu Yen-Chin , Berta Temugin , Liu Tong , Tan Ping-Heng , Ji Ru-Rong 
Published: March 2012

Background: Despite decades of intense research efforts, actions of acute opioids are not fully understood. Increasing evidence suggests that in addition to well-documented antinociceptive effects opioids also produce paradoxical hyperalgesic and excitatory effects on neurons. However, most studies focus on the pronociceptive actions of chronic opioid exposure. Matrix metalloproteinase 9 (MMP-9) plays an important role in neuroinflammation and neuropathic pain development. We examined MMP-9 expression and localization in dorsal root ganglia (DRGs) after acute morphine treatment and, furthermore, the role of MMP-9 in modulating acute morphine-induced analgesia and hyperalgesia in mice. Results: Subcutaneous morphine induced a marked up-regulation of MMP-9 protein in DRGs but not spinal cords. Morphine also increased MMP-9 activity and mRNA expression in DRGs. MMP-9 up-regulation peaked at 2 h but returned to the baseline after 24 h. In DRG tissue sections, MMP-9 is expressed in small and medium-sized neurons that co-express mu opioid receptors (MOR). In DRG cultures, MOR agonists morphine, DAMGO, and remifentanil each increased MMP-9 expression in neurons, whereas the opioid receptor antagonist naloxone and the MOR-selective antagonist D-Phe-Cys-Tyr-D-Trp-Arg-Thr-Pen-Thr-NH2 (CTAP) suppressed morphine-induced MMP-9 expression. Notably, subcutaneous morphine-induced analgesia was enhanced and prolonged in Mmp9 knockout mice and also potentiated in wild-type mice receiving intrathecal injection of MMP-9 inhibitors. Consistently, intrathecal injection of specific siRNA targeting MMP-9 reduced MMP-9 expression in DRGs and enhanced and prolonged morphine analgesia. Subcutaneous morphine also produced heat hyperalgesia at 24 h, but this opioid-induced hyperalgesia was not enhanced after MMP-9 deletion or inhibition. Conclusions: Transient MMP-9 up-regulation in DRG neurons can mask opioid analgesia, without modulating opioid-induced hyperalgesia. Distinct molecular mechanisms (MMP-9 dependent and independent) control acute opioid-induced pronociceptive actions (anti-analgesia in the first several hours and hyperalgesia after 24 h). Targeting MMP-9 may improve acute opioid analgesia.
Criteria for determining the need for surgical treatment of tricuspid regurgitation during mitral valve replacement
Authors: He Jigang , Shen Zhenya , Yu Yunsheng , Huang Haoyue , Ye Wenxue , Ding Yinglong , Yang Shaolei 
Published: March 2012

Abstract

Background
Tricuspid regurgitation (TR) is common in patients with mitral valve disease; however, there are no straightforward, rapidly determinably criteria available for deciding whether TR repair should be performed during mitral valve replacement. The aim of our retrospective study was to identify a simple and fast criterion for determining whether TR repair should be performed in patients undergoing mitral valve replacement.

Methods
We reviewed the records of patients who underwent mitral valve replacement with or without (control) TR repair (DeVega or Kay procedure) from January 2005 to December 2008. Preoperative and 2-year postoperative echocardiographic measurements included right ventricular and atrial diameter, interventricular septum size, TR severity, ejection fraction, and pulmonary artery pressure.

Results
A total of 89 patients were included (control, n = 50; DeVega, n = 27; Kay, n = 12). Demographic and clinical characteristics were similar between groups. Cardiac variables were similar between the DeVega and Kay groups. Right atrium and ventricular diameter and ejection fraction were significantly decreased postoperatively both in the control and operation (DeVega + Kay) group (P < 0.05). Pulmonary artery pressure was significantly decreased postoperatively in-operation groups (P < 0.05). Our findings indicate that surgical intervention for TR should be considered during mitral valve replacement if any of the following preoperative criteria are met: right atrial transverse diameter > 57 mm; right ventricular end-diastolic diameter > 55 mm; pulmonary artery pressure > 58 mmHg.

Conclusions
Our findings suggest echocardiography may be used as a rapid and simple means of determining which patients require TR repair during mitral valve replacement.
Indians in the Lobby: Newspapers and the Limits of Andean Cosmopolitanism, 1896–1930
Authors: Hiatt Willie 
Published: March 2012

JOSH: So there are these two Indians in the lobby … C. J.: Yeah? (waiting for the punch line) JOSH: No, that's not the beginning of a joke. I'm saying, … there are these two Indians in the lobby.Modernizers in Cuzco, Peru, ushered in the twentieth century by exalting newspapers as a universal vehicle for peace, prosperity, and progress. Although the city stood at more than 11,000 feet above sea level in the remote and rugged southern highlands, editors, public officials, and intellectuals were convinced that small but plentiful local newspapers contributed to a robust international public sphere. The writer who in 1910 lauded the press as die “aurora of salvation of the people” that “propagates itself through time and distance to keep redemptive thought alive” was hardly alone in his cosmopolitan idealism or emancipatory zeal. In the decades to come, a flood of pretentious self-tributes conveyed the idea that newspapers were almost divinely appointed to propagate a modern liberal project.
Non-anastomotic biliary strictures after liver transplantation: Focus on percutaneous treatment and extent of disease
Authors: Doros Attila , Deák Pál Ákos , Hartmann Erika , Németh Andrea , Gerlei Zsuzsa , Fazakas János , Görög Dénes , Nemes Balázs , Fehérvári Imre , Kóbori László 
Published: March 2012

Abstract

Introduction: Biliary strictures remain a key problem after liver transplantation. Anastomotic strictures are treated by surgery or interventional therapy. Intrahepatic stenosis requires retransplantation. For bridging, percutaneous and endoscopic interventions are used. The extent of the strictures may have an important role in therapy planning. Methods: Strictures were divided into four zones (1: extrahepatic, not included in this study; 2: hilar; 3: central; 4: peripheral). Twenty patients were treated with balloon dilatation/stent implantation/retransplantation/supportive care (Zone 1: 0/0/0/0; Zone 2: 8/7/2/0; Zone 3: 7/5/2/1; Zone 4: 1/1/3/1). Results: Mean follow-up time was 48 months. In Zone 2, one patient died as a result of recurrent hepatocellular carcinoma (HCC), and seven patients are alive, five after stent placements and two after retransplantation. Four patients are alive in Zone 3: all had stent placements and one later retransplantation. One patient died after retransplantation, two on the waiting list, and one due to chronic liver failure. One patient is alive in Zone 4 after early retransplantation, and three died. Conclusion: Percutaneous therapy is safe and effective in intrahepatic biliary stenosis after liver transplantation. It can provide the cure or bridge retransplantation. Based on zonal classification, we recommend the following treatments: Zone 4: early retransplantation; Zone 2: minimally invasive therapy; Zone 3: individual decisions.
Cytochrome P450-endogenous substrates metabolism is reduced in patients with a multiple organ dysfunction after coronary artery bypass grafting
Authors: Nepomniashchikh Valery , Lomivorotov Vladimir , Deryagin Michael , Lomivorotov Vladimir , Kniazkova Lubov 
Published: March 2012

Abstract

Aim: To evaluate endogenous toxic substrates and liver monooxygenase function in cardiosurgical patients with multiple organ dysfunction syndrome (MODS). Methods: 45 patients with MODS and 34 patients with an uneventful postoperative period were studied. The endogenous substrates were quantified with blood middle molecules (MM). Liver monooxygenase function was evaluated with antipyrine (AP) pharmacokinetics. Results: On the first postoperative day, MODS patients were characterized by high concentration of toxic substrates (MM: +43.8%) and a significant decrease in liver monooxygenase function (AP clearance: −44%), while controls patients had a mild increase in endogenous substrates and a slight depression in monooxygenase function. On the 3rd–4th postoperative day, in the main group, endogenous substrates increased (MM: +53.1%), while in the control group toxic substrates decreased (MM: +6.9%). In both groups, an increase in liver monooxygenase function was noticed. Major differences were observed on the 10th–12th postoperative day. In the main group, toxic substrates remained elevated (MM: +37.5%) and monooxygenase function was depressed (AP clearance: −45.4%), while in the control group endogenous substrates and monooxygenase function were equal to the baseline. The correlation analysis showed a negative relationship between AP pharmacokinetics and endogenous substrates. Conclusion: Slowdown in liver microsomal oxidation is one of the main reasons for the accumulation of endogenous toxic substrates in MODS cardiac patients.
Evaluation of zinc and selenium alterations in children with acute infections: Correlation with markers of inflammation
Authors: Karakonstantakis Theodoros , Papassotiriou Ioannis , Sergounioti Athanassia , Moraitis Panayiotis , Theodoridou Kalliopi , Afordakou Despoina , Giannaki Maria , Theodoridou Maria 
Published: March 2012

Abstract

Objectives: To investigate and assess selenium (Se) and zinc (Zn) serum alterations in children with acute infections. Material and Methods: We determined Se, Zn, C-reactive protein (CRP), serum amyloid A (SAA), and procalcitonin (PCT) in 80 children hospitalized with acute infections. The patients were divided in two groups: Bacterial infections: 40 children (mean age 4.8 years); viral infections: 40 children (mean age 5.7 years). Morning blood samples were collected from each patient in three different periods: (a) in the acute phase (admission), (b) during the inflammatory process (4th day of hospitalization), and (c) after recovery (28–33 days after the first sampling). Results: We found that patients with bacterial infections had significantly lower levels of Se (48.9±16.4 mg/L vs. 58.1±13.7 mg/L, p=0.014) and Zn (69.3±19.4 mg/dL vs. 79.9±13.1 mg/dL, p=0.008) than those with viral infections on admission. Zn and Se levels were significantly increased after remission and the 4th day of hospitalization in patients with bacterial infections. Se and Zn were inversely related with the inflammatory markers CRP (r=−0.480, p=0.002 and r=−0.380, p=0.02, respectively), and PCT (r=−0.670, p < 0.001 and r=−0.510, p<0.01, respectively), in the same patients group on admission. We also found a positive correlation between the two micronutrients (r = 0.400, p<0.01) in children with bacterial infections on admission. Conclusions: Zn and Se behave as negative acute phase reactants and their levels were inversely associated with the progress of infection in patients with bacterial infections. The changes of micronutrients (Se, Zn) during the inflammatory process are transient and resolve without any dietary intervention.
Inhibition of HBV-induced angiogenesis by ibuprofen: Role of HBx
Authors: Zhang Jianhua , Chen Wei Ning 
Published: March 2012

AbstractChronic hepatitis B virus (HBV) carriers may develop hepatocellular carcinoma (HCC) by a wide range of mechanisms including angiogenesis. We show that HBV replication induces the expression of angiogenic proteins interleukin 6 (IL6) and cyclooxygenase-2 (Cox2). Interestingly, ibuprofen (a Cox2 inhibitor) is found to attenuate the levels of IL6 and Cox 2 which are induced by HBV replication.The mechanism of attenuation of angiogenic proteins by ibuprofen was further investigated. Our results show that HBx is involved in the increase of the expression of Cox2 through the NFκB pathway. However, the expression of Cox2 is decreased when the HBx-expressing cells are incubated with ibuprofen. The contrasting effect of HBx on Cox2 is found to be determined by differential dimer formation among the members of the NFκB family of proteins, including NFκB, RelA, and C-rel. Specifically, HBx alone results in dimer formation between NFκB and RelA, while the combined presence of HBx and ibuprofen leads to the formation of NFκB and C-rel. Additional information on the interaction network involving HBx, ibuprofen, and NFκB pathways is revealed by two-dimensional liquid chromatography-tandem mass spectrometry proteomics analysis. Taken together, our findings provide new insights on the angiogenesis induced by HBV replication.
Increased expression of MDM2 and NM23 are associated with malignant transformation of pleomorphic adenoma
Authors: Zyada Manal M. 
Published: March 2012

Abstract
This study was designed to correlate the expression of both MDM2 and NM23 in pleomorphic adenoma (PA) to clinical background, histological features, local recurrence, and metastatic potentiality in this tumor. Expression of MDM2 and NM23 was studied immunohistochemically in 23 cases of PA. Our results demonstrated MDM2 and NM23 overexpression in almost all cases of PA. There was a significant difference of the MDM2 and NM23 mean values between benign PA and both carcinoma-ex PA and metastasizing PA. The results of this study show that the overexpression of NM23 and MDM2 oncoproteins could be used as reliable predictors of malignant progression as well as metastatic potentiality of PA.