bluuebunny/crossref_metadata_2025_split huggingface dataset:
Astrocyte Networks and Epilepsy: When Stars Collide
Authors: Wong Michael 
Published: July 2009

Loss of Astrocytic Domain Organization in the Epileptic Brain. Oberheim NA, Tian GF, Han X, Peng W, Takano T, Ransom B, Nedergaard M. J Neurosci 2008;28(13):3264–3276. Gliosis is a pathological hallmark of posttraumatic epileptic foci, but little is known about these reactive astrocytes beyond their high glial fibrillary acidic protein (GFAP) expression. Using diolistic labeling, we show that cortical astrocytes lost their nonoverlapping domain organization in three mouse models of epilepsy: posttraumatic injury, genetic susceptibility, and systemic kainate exposure. Neighboring astrocytes in epileptic mice showed a 10-fold increase in overlap of processes. Concurrently, spine density was increased on dendrites of excitatory neurons. Suppression of seizures by the common antiepileptic, valproate, reduced the overlap of astrocytic processes. Astrocytic domain organization was also preserved in APP transgenic mice expressing a mutant variant of human amyloid precursor protein despite a marked upregulation of GFAP. Our data suggest that loss of astrocytic domains was not universally associated with gliosis, but restricted to seizure pathologies. Reorganization of astrocytes may, in concert with dendritic sprouting and new synapse formation, form the structural basis for recurrent excitation in the epileptic brain. Astroglial Metabolic Networks Sustain Hippocampal Synaptic Transmission. Rouach N, Koulakoff A, Abudara V, Willecke K, Giaume C. Science 2008;322(5907):1551–1555. Astrocytes provide metabolic substrates to neurons in an activity-dependent manner. However, the molecular mechanisms involved in this function, as well as its role in synaptic transmission, remain unclear. Here, we show that the gap-junction subunit proteins connexin 43 and 30 allow intercellular trafficking of glucose and its metabolites through astroglial networks. This trafficking is regulated by glutamatergic synaptic activity mediated by AMPA receptors. In the absence of extracellular glucose, the delivery of glucose or lactate to astrocytes sustains glutamatergic synaptic transmission and epileptiform activity only when they are connected by gap junctions. These results indicate that astroglial gap junctions provide an activity-dependent intercellular pathway for the delivery of energetic metabolites from blood vessels to distal neurons.
The Fragile X Mental Retardation Protein: A Valuable Partner in the Battle against Epileptogenesis
Authors: Merlin Lisa R. 
Published: July 2009

Correction of Fragile X Syndrome in Mice. Dölen G, Osterweil E, Rao BSS, Smith GB, Auerbach BD, Chattarji S, Bear MF. Neuron 2007;56:955–962. Fragile X syndrome (FXS) is the most common form of heritable mental retardation and the leading identified cause of autism. FXS is caused by transcriptional silencing of the FMR1 gene that encodes the fragile X mental retardation protein (FMRP), but the pathogenesis of the disease is unknown. According to one proposal, many psychiatric and neurological symptoms of FXS result from unchecked activation of mGluR5, a metabotropic glutamate receptor. To test this idea we generated Fmr1 mutant mice with a 50% reduction in mGluR5 expression and studied a range of phenotypes with relevance to the human disorder. Our results demonstrate that mGluR5 contributes significantly to the pathogenesis of the disease, a finding that has significant therapeutic implications for fragile X and related developmental disorders. Limbic Epileptogenesis in a Mouse Model of Fragile X Syndrome. Qiu LF, Lu TJ, Hu XL, Yi YH, Liao WP, Xiong ZQ. Cereb Cortex 2009 in press. (doi:10.1093/cercor/bhn163) Fragile X syndrome (FXS), caused by silencing of the Fmr1 gene, is the most common form of inherited mental retardation. Epilepsy is reported to occur in 20–25% of individuals with FXS. However, no overall increased excitability has been reported in Fmr1 knockout (KO) mice, except for increased sensitivity to auditory stimulation. Here, we report that kindling increased the expressions of Fmr1 mRNA and protein in the forebrain of wild-type (WT) mice. Kindling development was dramatically accelerated in Fmr1 KO mice, and Fmr1 KO mice also displayed prolonged electrographic seizures during kindling and more severe mossy fiber sprouting after kindling. The accelerated rate of kindling was partially repressed by inhibiting N-methyl-D-aspartic acid receptor (NMDAR) with MK-801 or mGluR5 receptor with 2-methyl-6-(phenylethynyl)-pyridine (MPEP). The rate of kindling development in WT was not effected by MPEP, however, suggesting that FMRP normally suppresses epileptogenic signaling downstream of metabotropic glutamate receptors. Our findings reveal that FMRP plays a critical role in suppressing limbic epileptogenesis and predict that the enhanced susceptibility of patients with FXS to epilepsy is a direct consequence of the loss of an important homeostatic factor that mitigates vulnerability to excessive neuronal excitation.
Continuing Medical Education Program in Echocardiography
Authors: Fan Pohoey , Luckie Mathew , Irion Luciane , Khattar Rajdeep S. 
Published: July 2009

Article Title: Severe Mitral and Aortic Regurgitation in Association with Ankylosing Spondylitis(ECHOCARDIOGRAPHY, Volume 26, July 2009)
Immunohistochemical analysis of NF‐κB signaling proteins IKKε, p50/p105, p52/p100 and RelA in prostate cancers
Authors: SEO SEONG IL , SONG SANG YONG , KANG MI RAN , KIM MIN SUNG , OH JI EUN , KIM YOO RI , LEE JI YOUL , YOO NAM JIN , LEE SUG HYUNG 
Published: July 2009

Seo SI, Song SY, Kang MR, Kim MS, Oh JE, Kim YR, Lee JY, Yoo NJ, Lee SH. Immunohistochemical analysis of NF‐κB signaling proteins IKKε, p50/p105, p52/p100 and RelA in prostate cancers. APMIS 2009; 117:623–8.Activation of nuclear factor‐kappa B (NF‐κB) signaling is considered an important mechanism in the development of prostate cancers. A recent study revealed that IκB kinase epsilon (IKKε), an activator of NF‐κB, was overexpressed in breast cancers and acted as an oncogene. Expression of NF‐κB members has been reported in prostate cancer tissues, but expression of IKKε has not yet been studied in prostate cancers. In this study, we attempted to explore as to whether expressions of IKKε and NF‐κB members p50/105, p52/p100 and RelA are altered in prostate cancers. We analyzed the expression of IKKε, p50/105, p52/p100 and RelA in 107 prostate adenocarcinoma tissues by immunohistochemistry using a tissue microarray (TMA) method. In the TMA, IKKε is expressed in basal cells, but not in alveolar cells in normal prostate glands. IKKε is expressed in 60.0% of prostate intraepithelial neoplasm (PIN) and 70.1% of the prostate cancers in the cytoplasm. Nuclear immunostainings of NF‐κB members p50/105, p52/p100 and RelA, which are considered activation of NF‐κB signaling, were observed respectively in 28.0%, 18.7% and 37.4% of the cancers. Nuclear staining was detected neither in normal alveolar cells nor in PIN. However, none of the expression of p50/105 nor p52/p100 nor RelA nor IKKε was associated with pathologic characteristics, including size of the cancers, age, Gleason score and stage. The increased cytoplasmic expression of IKKε as well as the increased nuclear expressions of p50/105, p52/p100 and RelA in the prostate cancers compared to normal alveolar cells suggested that overexpression of these proteins may be related to activation of the NF‐κB pathway and might play a role in tumorigenesis of prostate cancers.
Predicting the incidence of human campylobacteriosis in Finland with time series analysis
Authors: SUMI AYAKO , HEMILÄ HARRI , MISE KEIJI , KOBAYASHI NOBUMICHI 
Published: July 2009

Sumi A, Hemilä H, Mise K, Kobayashi N. Predicting the incidence of human campylobacteriosis in Finland with time series analysis. APMIS 2009; 117: 614–22.Human campylobacteriosis is a common bacterial cause of gastrointestinal infections. In this study, we tested whether spectral analysis based on the maximum entropy method (MEM) is useful in predicting the incidence of campylobacteriosis in five provinces in Finland, which has been accumulating good quality incidence data under the surveillance program for water‐ and food‐borne infections. On the basis of the spectral analysis, we identified the periodic modes explaining the underlying variations of the incidence data in the years 2000–2005. The optimum least squares fitting (LSF) curve calculated by using the periodic modes reproduced the underlying variation of the incidence data. We extrapolated the LSF curve to the years 2006 and 2007 and predicted the incidence of campylobacteriosis. Our study suggests that MEM spectral analysis allows us to model temporal variations of the disease incidence with multiple periodic modes much more effectively than using the Fourier model, which has been previously used for modeling seasonally varying incidence data.
Detection of virulence genes of Clostridium difficile by multiplex PCR
Authors: ANTIKAINEN JENNI , PASANEN TANJA , MERO SOINTU , TARKKA EVELIINA , KIRVESKARI JUHA , KOTILA SAARA , MENTULA SILJA , KÖNÖNEN EIJA , VIROLAINEN‐JULKUNEN ANNI‐RIITTA , VAARA MARTTI , TISSARI PÄIVI 
Published: July 2009

Antikainen J, Pasanen T, Mero S, Tarkka E, Kirveskari J, Kotila S, Mentula S, Könönen E, Virolainen‐Julkunen A‐R, Vaara M, Tissari P. Detection of virulence genes of Clostridium difficile by multiplex PCR. APMIS 2009; 117: 607–13. Clostridium difficile strains belonging to the PCR ribotype 027, pulse‐field gel electrophoresis (PFGE) type NAP1, toxinotype III and restriction endonuclease analysis group BI harbouring mutations in the tcdC gene and possessing binary toxin components A and B have been described to cause epidemics with increased morbidity and mortality. In the present study we developed a conventional multiplex PCR designed to detect selected virulence associated markers of the hypervirulent C. difficile PCR ribotype 027. The multiplex PCR assay detected the major toxins A and B, binary toxin components A and B as well as a possible deletion in the tcdC gene: a characteristic pattern of amplification products for the PCR ribotype 027 strains was detected. This rather simple method was specific for the screening of this hypervirulent C. difficile strain. The correlation between the multiplex PCR and PCR ribotyping methods was excellent. The sensitivity and specificity were 100% in our epidemiological situation. In conclusion, this multiplex PCR was found useful in the preliminary screening for the hypervirulent C. difficile PCR ribotype 027.
Psoriasis vs allergic contact dermatitis in palms and soles: a quantitative histologic and immunohistochemical study
Authors: CESINARO ANNA MARIA , NANNINI NAZARENA , MIGALDI MARIO , PEPE PATRIZIA , MAIORANA ANTONIO 
Published: July 2009

Cesinaro AM, Nannini N, Migaldi M, Pepe P, Maiorana A. Psoriasis vs allergic contact dermatitis in palms and soles: a quantitative histologic and immunohistochemical study. APMIS 2009; 117: 629–34.A systematic histologic and immunohistochemical study of cases of psoriasis (PSO) and allergic contact dermatitis (ACD) in palmo‐plantar skin was performed to find differences between these two diseases that usually show overlapping features in these specific sites. Skin biopsies from 42 (22 female, 20 male) patients were evaluated for several histopathologic parameters and immunohistochemistry was applied to quantify keratinocytic proliferation, the number of dendritic cells (DCs) and the phenotype of the mononuclear cell infiltrate. Regular epidermal hyperplasia and marked parakeratosis were found to be more frequent in PSO than in ACD cases, but only the first parameter reached the level of significance (p = 0.03). The number of S100 protein‐positive DCs was significantly higher in ACD (p = 0.006), whereas keratinocytic proliferation, studied with Mib‐1, was found to be higher in PSO than in ACD, but the difference was not statistically significant. No significant difference was detected in the number of CD4+, CD8+ and bcl2+ lymphocytes in PSO and ACD cases. In the palms and soles, the finding of irregular epidermal hyperplasia and the detection of a higher number of S100 protein‐positive DCs favor the diagnosis of ACD over PSO. The differential diagnosis between PSO and ACD can be practically approached using a histopathologic parameter and a commercially available antibody.
Conservation Value of Landscape Supplementation for Howler Monkeys Living in Forest Patches
Authors: Asensio Norberto , Arroyo‐Rodríguez Víctor , Dunn Jacob C. , Cristóbal‐Azkarate Jurgi 
Published: July 2009

ABSTRACTMany animal populations are forced to inhabit very small forest patches, which may threaten their long‐term survival. In some cases, animals in these forest remnants are able to supplement their diet by using resources outside of their home patch, a process named ‘landscape supplementation’. Although this is probably a key process for population survival in fragmented landscapes, little is known about the ability of most animal species to move through the matrix and feed from different landscape elements. In this paper we report several cases of landscape supplementation by two groups of Mexican mantled howler monkeys Alouatta palliata mexicana inhabiting two different forest patches in Los Tuxtlas, Mexico. Our observations show that howler monkeys used several landscape elements, such as isolated trees, live fences and neighboring forest patches to supplement their diet. These observations underline the importance of these landscape elements for the survival of forest‐dependent animals in highly fragmented landscapes, as they can provide important food resources and, hence, can be considered as potential extensions of their home range. However, the degree of protection the landowner provides to howler monkeys and proper management of these landscape elements are key aspects in determining the use of these elements by these animals.
The effect of stellar rotation on colour–magnitude diagrams: on the apparent presence of multiple populations in intermediate age stellar clusters
Authors: Bastian N. , De Mink S. E. 
Published: July 2009

Abstract
A significant number of intermediate age clusters (1–2 Gyr) in the Magellanic Clouds appear to have multiple stellar populations within them, derived from bimodal or extended main-sequence turn-offs. If this is interpreted as an age spread, the multiple populations are separated by a few hundred million years, which would call into question the long-held notion that clusters are simple stellar populations. Here, we show that stellar rotation in stars with masses between 1.2 and 1.7 M⊙ can mimic the effect of a double or multiple population, whereas in actuality only a single population exists. The two main causes of the spread near the turn-off are the effects of stellar rotation on the structure of the star and the inclination angle of the star relative to the observer. Both effects change the observed effective temperature, hence colour, and flux of the star. In order to match observations, the required rotation rates are 20–50 per cent of the critical rotation, which are consistent with observed rotation rates of similar mass stars in the Galaxy. We provide scaling relations which can be applied to non-rotating isochrones in order to mimic the effects of rotation. Finally, we note that rotation is unlikely to be the cause of the multiple stellar populations observed in old globular clusters, as low-mass stars (&lt;1 M⊙) are not expected to be rapid rotators.
Myeloablative hematopoietic stem cell transplantation for myelodysplastic syndrome in patients younger than 55 years: impact of comorbidity and disease burden on the long‐term outcome
Authors: KANAMORI H. , ENAKA M. , ITO S. , MOTOHASHI K. , HAGIHARA M. , OSHIMA R. , SAKAI R. , FUJISAWA S. , TANAKA M. , FUJIMAKI K. , FUJITA H. , ISHIGATSUBO Y. , MARUTA A. 
Published: July 2009

SummaryWe retrospectively investigated 31 myelodysplastic syndrome (MDS) patients receiving myeloablative hematopoietic stem cell transplantation (HCT) and focused on prognostic factors affecting the long‐term outcome. Patients were classified according to the French‐American‐British classification and the HCT‐comorbidity index was determined. Cytosine arabinoside or thiotepa combined with cyclophosphamide and total body irradiation was used as myeloablative conditioning in eight and 23 patients respectively. After a follow‐up period of 0.8–14.2 years from transplantation (median: 6.4 years), 23 patients were alive in complete remission, and the 5‐year overall survival (OS) and disease‐free survival (DFS) rates were 79% and 72% respectively. The cumulative nonrelapse mortality (NRM) rate was 22% at 5 years. According to multivariate analysis, ≥20% blasts in the bone marrow and an HCT‐comorbidity score ≥ 3 were significantly associated with poor OS and DFS. Patients with a high HCT‐comorbidity score and male patients receiving transplantation from female donors were significantly more likely to have a higher NRM according to the univariate, but not the multivariate analysis. These data suggest that comorbidity and the tumor burden at the time of transplantation may be useful variables for predicting the outcome in MDS patients receiving myeloablative HCT.
Conjoint Measurement with Constraints on Attribute Levels: A Mixture–Amount Model Approach
Authors: Raghavarao Damaraju , Wiley James B. 
Published: July 2009

SummaryTraditional and choice‐based conjoint analyses (CAs) have used full or fractional factorial designs to generate concept profile descriptions. However, these designs confound two factors when costs are associated with attributes: first is the total cost of the concept profile, and second is the allocation of costs among the attributes. Both factors may influence consumers' preferences. So far, these issues have not been separated in the CA literature. The present paper shows how mixture–amount designs used in industrial experiments may be used to separate amount and mixture effects in traditional CA. The extension to choice‐based CA using balance incomplete block (BIB) designs is also given.
Spatiotemporal changes in the genetic diversity in French bloom‐forming populations of the toxic cyanobacterium, Microcystis aeruginosa
Authors: Sabart Marion , Pobel David , Latour Delphine , Robin Joel , Salençon Marie‐J. , Humbert Jean‐F. 
Published: July 2009

Summary Microcystis aeruginosa is a toxic cyanobacterium, which is able to bloom in a wide range of freshwater ecosystems. By sequencing the Internal Transcribed Spacer (ITS) of the ribosomal operon, we compared the genetic composition of several French bloom‐forming M. aeruginosa populations from two reservoirs located on the Loire River, at two sampling points located between these reservoirs, and finally in two ponds closely linked to this river. No significant difference was found in the genetic diversity of the six Microcystis populations but we evidenced a strong genetic differentiation between most of these populations. Indeed, the Microcystis population in the Grangent reservoir was genetically differentiated from the other three populations sampled further downstream, implying that no massive transfer of population occurs from this reservoir to downstream segments. We also found genetic differentiation between the populations from the two ponds, and between these populations and those from the Loire River. On the other hand, the same dominant genotype was found in the populations sampled both in the river and in the Villerest reservoir, suggesting the selection of a distinct genotype adapted to river conditions and also an accumulation of this genotype in the downstream reservoir. Finally, by comparing our ITS sequences with those available in the GenBank, no biogeographical differentiation could be detected at a global scale, suggesting that most of the Microcystis genotypes seem to be ubiquitous.
Performance of the Epstein-Barr Virus and Herpes Simplex Virus Immunoglobulin M Assays on the Liaison Platform with Sera from Patients Displaying Acute Parvovirus B19 Infection
Authors: Costa Elisa , Tormo Nuria , Clari María Ángeles , Bravo Dayana , Muñoz-Cobo Beatriz , Navarro David 
Published: July 2009

ABSTRACT
Acute parvovirus B19 infection has been reported to cause false-positive results frequently in the Epstein-Barr (EBV) and herpes simplex virus (HSV) immunoglobulin M (IgM) assays from DiaSorin performed on the Liaison platform. We tested 65 sera from patients with a presumptive or conclusive diagnosis of acute parvovirus B19 infection in both assays and obtained no false-positive results in the EBV IgM test and 10.4% nonspecific reactivities in the HSV IgM assay. Our data support the specificity of both assays in this clinical setting.
An Oral
Mycobacterium bovis
BCG Vaccine for Wildlife Produced in the Absence of Animal-Derived Reagents
Authors: Cross Martin L. , Lambeth Matthew R. , Aldwell Frank E. 
Published: July 2009

ABSTRACT

Cultures of
Mycobacterium bovis
BCG, comprising predominantly single-cell bacilli, were prepared in broth without animal-derived reagents. When formulated into a vegetable-derived lipid matrix, the vaccine was stable in vitro and was immunogenic in vivo upon feeding it to mice. This formulation could be useful for oral vaccination of wildlife against tuberculosis, where concern over transmissible prions may preclude the field use of vaccines containing animal products.
Genetic Characterization ofClostridium botulinumAssociated with Type B Infant Botulism in Japan
Authors: Umeda Kaoru , Seto Yoshiyuki , Kohda Tomoko , Mukamoto Masafumi , Kozaki Shunji 
Published: July 2009

ABSTRACTThe 15 proteolyticClostridium botulinumtype B strains, including 3 isolates associated with infant botulism in Japan, were genetically characterized by phylogenetic analysis ofboNT/Bgene sequences, genotyping, and determination of theboNT/Bgene location by using pulsed-field gel electrophoresis (PFGE) for molecular epidemiological analysis of infant botulism in Japan. Strain Osaka05, isolated from a case in 2005, showed a uniqueboNT/Bgene sequence and was considered to be a new BoNT/B subtype by phylogenetic analysis. Strain Osaka06, isolated from a case in 2006, was classified as the B2 subtype, the same as strain 111, isolated from a case in 1995. The five isolates associated with infant botulism in the United States were classified into the B1 subtype. Isolates from food samples in Japan were divided into the B1 and the B2 subtypes, although no relation with infant botulism was shown by PFGE genotyping. The results of PFGE and Southern blot hybridization with undigested DNA suggested that theboNT/Bgene is located on large plasmids (approximately 150 kbp, 260 kbp, 275 kbp, or 280 kbp) in five strains belonging to three BoNT/B subtypes from various sources. The botulinum neurotoxin (BoNT) of Osaka05 was suggested to have an antigenicity different from the antigenicities of BoNT/B1 and BoNT/B2 by a sandwich enzyme-linked immunosorbent assay with the recombinant BoNT/B-C-terminal domain. We established a multiplex PCR assay for BoNT/B subtyping which will be useful for epidemiological studies of type B strains and the infectious diseases that they cause.
Evaluation of a New Rapid Antigen Test Using Immunochromatography for Detection of Human Metapneumovirus in Comparison with Real-Time PCR Assay
Authors: Matsuzaki Yoko , Takashita Emi , Okamoto Michiko , Mizuta Katsumi , Itagaki Tsutomu , Katsushima Fumio , Katsushima Yuriko , Nagai Yukio , Nishimura Hidekazu 
Published: July 2009

ABSTRACTA new rapid human metapneumovirus (hMPV) detection kit using immunochromatography (SAS hMPV test) was compared to real-time PCR for 224 nasal swab specimens, 96.4% of which were obtained from children of <15 years of age. The overall sensitivity and specificity were 82.3% and 93.8%, respectively, suggesting that this test is useful for pediatricians to diagnose hMPV infection in a clinical setting.