bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unresolved ethical issues of genetic counseling and testing in clinical psychiatry
Authors: Perry Julia , Bunnik Eline , Rietschel Marcella , Bentzen Heidi Beate , Ingvoldstad Malmgren Charlotta , Pawlak Joanna , Chaumette Boris , Tammimies Kristiina , Bialy Filip , Bizzarri Virginia , Borg Isabella , Coviello Domenico , Crepaz-Keay David , Ivanova Eliza , McQuillin Andrew , Mežinska Signe , Johansson Soller Maria , Suvisaari Jaana , Watson Melanie , Wirgenes Katrine , Wynn Sarah L. , Degenhardt Franziska , Schicktanz Silke 
Published: February 2025

Objective
This position article discusses current major ethical and social issues related to genetic counseling and testing in clinical psychiatry (PsyGCT).

Methods
To address these complex issues in the context of clinical psychiatry relevant to PsyGCT, the interdisciplinary and pan-European expert Network EnGagE (Enhancing Psychiatric Genetic Counseling, Testing, and Training in Europe; CA17130) was established in 2018. We conducted an interdisciplinary, international workshop at which we identified gaps across European healthcare services and research in PsyGCT; the workshop output was summarized and systematized for this position article.

Results
Four main unresolved ethical topics were identified as most relevant for the implementation of PsyGCT: (1) the problematic dualism between somatic and psychiatric disorders, (2) the impact of genetic testing on stigma, (3) fulfilling professional responsibilities, and (4) ethical issues in public health services. We provide basic recommendations to inform psychiatrists and other healthcare professionals involved in the clinical implementation of PsyGCT and conclude by pointing to avenues of future ethics research in PsyGCT.

Conclusion
This article draws attention to a set of unresolved ethical issues relevant for mental health professionals, professionals within clinical genetics, patients and their family members, and society as a whole and stresses the need for more interdisciplinary exchange to define standards in psychiatric counseling as well as in public communication. The use of PsyGCT may, in the future, expand and include genetic testing for additional psychiatric diagnoses. We advocate the development of pan-European ethical standards addressing the four identified areas of ethical–practical relevance in PsyGCT.
Chronic Pain in Parkinson’s Disease: Prevalence, Sex Differences, Regional Anatomy, and Multiple Comorbidities
Authors: Ogonowski Natalia S. , Chafota Freddy , Cao Fangyuan , Yin Lim Amanda Wei , Flores-Ocampo Victor , Díaz-Torres Santiago , Ceja Zuriel , García-Marín Luis M. , Farrell Scott F. , Kumar Kishore R. , Alty Jane , Mellick George D. , Ngô Trung Thành , Renteria Miguel E. 
Published: February 2025

ABSTRACTChronic pain (pain on most days or daily for ≥3 months) is prevalent among people living with Parkinson’s disease (PD). We analysed data from 10,631 Australian individuals with PD to assess the prevalence, severity, anatomical distribution, clinical history, and associated factors. Two-thirds (66.2%) reported chronic pain, with females experiencing higher prevalence (70.8%) and severity (4.7 vs. 4.3 on a 10-point scale). Common pain sites included the buttocks (35.6%), lower back (25.4%), neck (19.4%), and knees (17.2%). Chronic pain was strongly linked to comorbid depression, sleep disorders, and osteoarthritis (p < 0.05). Environmental exposures such as pesticides, heavy metals, and alcohol were associated with higher pain prevalence, especially in males (p < 0.05). These findings emphasise the substantial burden of chronic pain in PD, highlighting sex differences and strong links to multimorbidity. Further research is warranted to clarify sex-specific treatments and identify novel therapeutic targets.
Systematic evaluation of medication adherence determinants across 137 ingredients on population-level real-world health data
Authors: Mooses Kerli , Oja Marek , Malk Maria , Loorents Helene , Pajusalu Maarja , Umov Nikita , Tamm Sirli , Holm Johannes , Keidong Hanna , Tillmann Taavi , Reisberg Sulev , Vilo Jaak , Kolde Raivo 
Published: February 2025

AbstractThe current knowledge about medication adherence (MA) is based on studies focusing only on few health conditions. We utilised a representative dataset with electronic health records, claims, and prescribed medications to 1) evaluate the effect of multiple factors affecting MA in a consistent manner across 137 ingredients, and 2) calculate individual medication adherence score (IMAS), evaluate its predictive power, stability over time, and impact on health outcomes. The MA ranged from 0.423 (albuterol, 95% CI 0.414–0.432) to 0.922 (warfarin, 95% CI 0.917–0.926). The demographic, health- and medication-related factors explained 11.6% and IMAS 22% of the variation in adherence. IMAS predicted adherence across medication classes, reduced the risk of overall hospitalisation (Hazard ratio = 0.76, 95% CI 0.60–0.97, p<0.05) and cause-specific incidence for 17 conditions. Thus, medication-taking behaviour represents a broader patient-level phenomenon manifesting consistently across medications, suggesting its potential for personalised interventions in clinical practice and more efficient public health strategies and policies.
TAC-C uncovers open chromatin interaction in crops and SPL-mediated photosynthesis regulation
Authors: Kang Jingmin , Zhang Zhaoheng , Lin Xuelei , Liu Fuyan , Song Yali , Zhao Peng , Lin Yujing , Luo Xumei , Li Xiaoyi , Li Yanyan , Wang Wenda , Liu Cuimin , Xu Shengbao , Liu Xin , Xiao Jun 
Published: February 2025

AbstractCis-regulatory elements (CREs) direct precise gene expression for development and environmental response, yet their spatial organization in crops is largely unknown. We introduce Transposase-Accessible Chromosome Conformation Capture (TAC-C), a method integrating ATAC-seq and Hi-C to capture fine-scale chromatin interactions in four major crops: rice, sorghum, maize, and wheat. TAC-C reveals that chromatin interaction frequency aligns with genome size and gene expression, exhibiting distinct loop structures between C3 and C4 plants, particularly in C4-specific enzymes coding genes. Integrating chromatin structure with population genetics data highlights that chromatin loops connect distal regulatory elements to phenotypic variation. Asymmetrical open chromatin interactions among subgenomes, driven by transposon insertions and sequence variations, contribute to biased homoeolog expression. Furthermore, TaSPL7/15 regulate photosynthesis-related genes through chromatin interactions, with enhanced photosynthetic efficiency and starch content inTaspl7&15mutant. TAC-C provides new insights into the spatial organization of regulatory elements in crops, especially for SPL-mediated photosynthesis regulation in wheat.
ELECTROPHYSIOLOGICAL CORRELATES OF CONSCIOUS EXPERIENCES DURING SLEEP: LUCID DREAMS, SLEEP PARALYSIS, OUT-OF-BODY EXPERIENCES, AND FALSE AWAKENINGS
Authors: Herrero Nerea L. , Corfdir Yohann , Vázquez-Chenlo Aylin A. , Capurro Lucila , Forcato Cecilia 
Published: February 2025

AbstractConsciousness does not always fade during sleep. Instead, it can re-emerge, giving rise to lucid dreams (LDs), sleep paralysis (SP), out-of-body experiences (OBEs), and false awakenings (FAs). While some of these states have been studied phenomenologically, their neurophysiological underpinnings remain unclear.Here, we investigate their electrophysiological correlates and distinguish them from standard sleep stages. We conducted overnight polysomnography in frequent experiencers, capturing 10 episodes (3 LDs, 2 SP, 2 OBEs, 3 FAs). Eye movement markers identified periods of lucidity. Relative spectral power was analyzed using principal component analysis (PCA) and permutation-based multivariate analysis of variance (PERMANOVA).Our results indicate that these conscious sleep states are distinct from wakefulness, yet share features with both stage 1 (S1) and rapid eye movement (REM) sleep. Notably, we provide the first documented eye movement markers during FAs and OBEs.
A Complete Spatial Map of Mouse Retinal Ganglion Cells Reveals Density and Gene Expression Specializations
Authors: Budoff Samuel A. , Poleg-Polsky Alon 
Published: February 2025

ABSTRACTRetinal ganglion cells (RGCs) transmit visual information from the eye to the brain. In mice, several RGC subtypes show nonuniform spatial distributions, potentially mediating specific visual functions. However, the full extent of RGC specialization remains unknown. Here, we used en-face cryosectioning, spatial transcriptomics, and machine learning to map the spatial distribution of all RGC subtypes identified in previous single-cell studies. While two-thirds of RGC subtypes were evenly distributed, others showed strong biases toward ventral or dorso-temporal regions associated with sky vision and the area retinae temporalis (ART), the predicted homolog of the area centralis. Additionally, we observed unexpected spatial variation in gene expression within several subtypes along the dorso-ventral axis or within vs. outside the ART, independent of RGC density profiles. Finally, we found limited correlations between the gene profiles of the ART and the primate macula, suggesting divergent specialization between the mouse and primate central vision.
Direct-view oblique plane microscopy
Authors: Lamb Jacob R. , Mestre Miguel Cardoso , Lancaster Madeline , Manton James D. 
Published: February 2025

AbstractThe ability to rapidly image mesoscopic samples is critically important for many areas of biological research. Owing to its high through-put and gentle, volumetric imaging nature, light-sheet fluorescence microscopy (LSFM) is an attractive modality to image such samples. However, the orthogonal dual-objective geometry of LSFM makes sample mounting challenging. Oblique plane microscopy (OPM) circumvents these issues by achieving light-sheet imaging through a single objective near the sample, with a further two objectives acting to image a tilted, oblique plane in the specimen. However, at low magnification, as required for mesoscopic imaging, conventional OPM systems suffer from a complicated and inefficient light path and/or a limited numerical aperture. Here we present a simple method for mesoscopic OPM that enables efficient light collection at any numerical aperture. By placing the camera directly in the remote space, the oblique plane can be imaged without the need for a third microscope system. Using a commercially available camera with 1.4 µm pixels, we demonstrate imaging over a 5.3 × 3 × 2.6 mm3field of view with a lateral resolution of ∼2 µm and axial resolution of ∼22 µm. Our technique is then used to image a 4×-expanded brain organoid.
Specificities of Chemosensory Receptors in the Human Gut Microbiota
Authors: Xu Wenhao , Jalomo-Khayrova Ekaterina , Gumerov Vadim M , Ross Patricia A. , Köbel Tania S. , Schindler Daniel , Bange Gert , Zhulin Igor B. , Sourjik Victor 
Published: February 2025

AbstractThe human gut is rich in metabolites and harbors a complex microbial community, yet the sensory repertoire of its commensal bacteria remains largely uncharacterized. Here we systematically mapped ligand specificities of extracytoplasmic sensory domains from twenty members of the human gut microbiota, with a primary focus on the abundant and physiologically important class of Clostridia. We identified diverse metabolites as specific stimuli for three major functional classes of transmembrane receptors. We further characterized novel subsets of sensors belonging to the Cache superfamily, specific for lactate, dicarboxylic acids, and for uracil and short-chain fatty acids (SCFAs), respectively, and investigated the evolution of their ligand specificity. Structural and biochemical analysis of the newly described dCache_1UR domain revealed an independent binding of uracil and SCFA at distinct modules. Altogether, we could identify or predict specificities for over a half of the Cache-type chemotactic sensors in the selected gut commensals, with the carboxylic acids representing the largest class of ligands. Among those, the most commonly found specificities were for lactate and formate, indicating particular importance of these metabolites in the human gut microbiome and consistent with their observed beneficial impact on the growth of selected bacterial species.
Genomic Insights into Ceftazidime Resistance inBurkholderia pseudomallei: Discovery of A172T Mutation, and Palindromic GC-Rich Repeat Sequences FacilitatingpenADuplication and Amplification
Authors: Tuanyok Apichai , Nakajima Chie , Noll Tiernan , Khan Md. Siddiqur Rahman , Khrongsee Pacharapong , Yowell Charles A. , Xiao Yu-Ping , Wuthiekanun Vanaporn , Chantratita Narisara , Heine Henry , Subramaniam Kuttichantran , Suzuki Yasuhiko , Limmathurotsakul Direk , Mergia Ayalew 
Published: February 2025

ABSTRACTCeftazidime (CAZ) resistance inBurkholderia pseudomallei, the causative agent of melioidosis, complicates treatment in endemic regions. This study identified a novel A172T mutation and other knownpenAmutations as critical contributors to CAZ resistance in a large Thai strain collection. Frequent gene duplication and amplification (GDA) ofpenA, likely driven by Palindromic GC-Rich Repeat Sequences (PGCRRS), highlights the urgent need for rapid diagnostics and optimized treatment strategies to manage this life-threatening disease effectively.
TDP-43 Aggregate Seeding Impairs Autoregulation and Causes TDP-43 Dysfunction
Authors: Mamede Lohany Dias , Hu Miwei , Titus Amanda R. , Vaquer-Alicea Jaime , French Rachel L. , Diamond Marc I. , Miller Timothy M. , Ayala Yuna M. 
Published: February 2025

ABSTRACTThe aggregation, cellular mislocalization and dysfunction of TDP-43 are hallmarks of multiple neurodegenerative disorders. We find that inducing TDP-43 aggregation through prion-like seeding gradually diminishes normal TDP-43 nuclear localization and function. Aggregate-affected cells show signature features of TDP-43 loss of function, such as DNA damage and dysregulated TDP-43-target expression. We also observe strong activation of TDP-43-controlled cryptic exons in cells, including human neurons treated with proteopathic seeds. Furthermore, aggregate seeding impairs TDP-43 autoregulation, an essential mechanism controlling TDP-43 homeostasis. Interestingly, proteins that normally interact with TDP-43 are not recruited to aggregates, while other factors linked to TDP-43 pathology, including Ataxin 2, specifically colocalize to inclusions and modify seeding-induced aggregation. Our findings indicate that TDP-43 aggregation, mislocalization and loss of function are strongly linked and suggest that disruption of TDP-43 autoregulation establishes a toxic feed-forward mechanism that amplifies aggregation and may be central in mediating this pathological connection.
Long-lived sterile neutrinos from an axionlike particle at Belle II
Authors: Wang Zeren Simon , Zhang Yu , Liu Wei 
Published: February 2025

Axionlike particles (ALPs) can be produced in meson decays via tree-level quark-flavor-violating couplings, and decay to a pair of sterile neutrinos if also coupled to them. Such light sterile neutrinos are necessarily long-lived and may give rise to striking signatures of displaced vertices (DVs) at terrestrial experiments. In this work, we study the prospect of the ongoing Belle II experiment for detecting the sterile neutrinos from the ALP, leveraging the O(1010) B-mesons projected to be produced at the experiment with an integrated luminosity of 50  ab−1. We propose search strategies for one or two DVs, and perform Monte-Carlo simulations in order to estimate the sensitivity reach of Belle II to active-sterile-neutrino mixing |VeN|2 as functions of the sterile-neutrino mass. Signatures include a selected list of sterile-neutrino final states, for which we estimate an O(1) or lower background level. Our results show that the proposed search strategies can probe values of |VeN|2 up to about two orders of magnitude beyond the existing bounds, for ALP mass over 4 GeV and sterile-neutrino mass above the D-meson threshold. Compared to the one-DV search, the two-DV one, despite its weaker sensitivities as a result of double exponential suppression of the sterile-neutrino decay positions, possesses the advantage of possible full reconstruction of the signal event allowing for pinning down the masses of the sterile neutrino and the ALP, if a discovery is made.

Published by the American Physical Society
2025
Consumers’ engagement in online shopping platform: influences on self-concept and subjective well-being within the platform
Authors: Lee Ha Kyung , Kim Woo Bin , Choo Ho Jung 
Published: February 2025

PurposeShopping through e-commerce platforms has become a primary daily activity. However, research on consumer engagement within e-commerce platform contexts remains scarce. We examine the relationship between consumer engagement on online shopping platforms and their subjective well-being, considering self-expansion and self-extension as mediators.Design/methodology/approachWe investigate the role of consumer engagement by dividing it into two experiences (crowdsourcing and crowdsending). Using validated measurement scales to analyze data from 440 South Korean consumers, we examine how these engagement experiences affect self-expansion and self-extension, ultimately leading to higher subjective well-being.FindingsCrowdsourcing and crowdsending play different and complementary roles in improving self-concept. Furthermore, self-expansion and self-extension are key variables influencing consumer engagement and well-being on the platform.Originality/valueThis study provides a new perspective of consumer online shopping behavior, revealing the self-related mechanisms that influence the relationship between consumer engagement experiences and subjective well-being.
Factors influencing consumers' willingness to pay a price premium and purchase frequency for organic food: a generational difference perspective
Authors: Lin Qiaoling , Jiang Hongbo , Luo Xi , Li Yingxia 
Published: February 2025

PurposeThis study applies the stimulus-organism-response (SOR) model and the theory of consumption value to examine the effects of natural content, environmental concern, and availability on consumers' willingness to pay a price premium and purchase frequency for organic food. It also ranks the relative importance of these factors and assesses generational differences.Design/methodology/approachUsing the purposive sampling to survey 435 Gen-Y and Gen-Z consumers in Mainland China. The data were analyzed using partial least squares structural equation modeling.FindingsThe results indicate that natural content, environmental concern, and availability are significant in predicting green consumption value. Additionally, green consumption value significantly affects the willingness to pay a price premium and purchase frequency. Furthermore, the willingness to pay a price premium significantly impacts purchase frequency. Finally, generational differences were confirmed in natural content-green consumption value, green consumption value-purchase frequency linkages.Originality/valueThis study contributes to the organic food literature by exploring how green consumption value influences the willingness to pay a price premium and purchase frequency. Additionally, it enriches the SOR model and the theory of consumption value by identifying generational differences that affect the strength of these relationships.
Firm-level climate risk exposure, ESG disclosure and stock liquidity. Evidence from textual analysis
Authors: Sahu Asis Kumar , Debata Byomakesh 
Published: February 2025

PurposeThis study examines the impact of firm-level climate risk exposure (FCRE) on firm stock liquidity by using a sample of Indian-listed firms from the financial years 2003–2004 to 2022–2023. Further, it endeavors to investigate the moderating role of environmental, social and governance (ESG) disclosure in this relationship.Design/methodology/approachA novel text-based FCRE metric is introduced using a sophisticated Word2Vec model through a Python-generated algorithm for each firm and year based on the management discussions and analysis (MD&A) reports. The panel fixed effect model is used to study how FCRE affects stock liquidity.FindingsThe result shows that FCRE negatively affects firms’ stock liquidity, and the effect remains robust after addressing endogeneity concerns. In addition, we find that a high ESG disclosure rating significantly moderated the adverse effect of FCRE. Furthermore, our analysis reveals that investor sentiment, information quality, corporate life cycle and institutional holdings moderate the impact of FCRE on liquidity.Practical implicationsThe study offers valuable insights for investors, managers and policymakers on integrating climate risk into investment strategies, improving corporate climate governance and shaping policies that incentivize sustainable corporate behavior.Originality/valueTo the best of our knowledge, this study is an early study to explore the relationship between firm-specific climate risk exposure and stock liquidity using advanced machine learning techniques. It contributes to the existing literature by illustrating how climate risk can lead to adverse market reactions while highlighting the critical roles of corporate ESG practices, investor sentiment and disclosure quality in influencing this relationship.
Sweet & Coffee in Ecuador: the challenge of market expansion
Authors: Gulawani Makarand , Sempèrtegui Seminario Carlos Alberto , Bodolica Virginia 
Published: February 2025

Learning outcomes
After working through the case and the assignment questions, students will be able to:
▪ Examine Ecuador’s business environment where coffee shops and similar companies operate.
▪ Evaluate the marketing challenges for an enterprise, particularly for a café business operating in Ecuador.
▪ Explain the marketing strategy for a café company to attract a variety of new consumer segments domestically and abroad.
▪ Discuss relevant international market entry strategies given the specificities of the environment in which a company operates.
▪ Describe the advantage of contemporary marketing tools in sustainable market expansion of a café business.

Case overview/synopsis
Richard Peet and Soledad Hanna turned their coffee shop business, Sweet & Coffee, into a flagship brand in Ecuador. Their coffee shops successfully promoted the culture of consuming coffee and sweets throughout Ecuador and grew exponentially to 129 stores. However, Sweet & Coffee faced significant challenges entering new states in Ecuador, with considerable investment in central kitchens and logistics. Despite the challenges, Peet wanted to continue opening new Sweet & Coffee stores across Ecuador. However, owing to Ecuador’s fast-changing and unpredictable external environment, Peet needed to make new adjustments to its marketing strategy to reposition Sweet & Coffee for a bright future. International market expansion was an option.

Complexity academic level
This case is helpful for advanced undergraduate or graduate courses in marketing and strategy.

Supplementary material
Teaching notes are available for educators only.

Subject code
CSS 8: Marketing.