bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparative phosphoproteomic analysis of a human tumor metastasis model using a label‐free quantitative approach
Authors: Xie Xiaolei , Feng Shun , Vuong Huy , Liu Yashu , Goodison Steve , Lubman David M. 
Published: May 2010

AbstractAlterations in cellular phosphorylation patterns have been implicated in a number of diseases, including cancer, through multiple mechanisms. Herein we present a survey of the phosphorylation profiles of an isogenic pair of human cancer cell lines with opposite metastatic phenotype. Phosphopeptides were enriched from tumor cell lysates with titanium dioxide and zirconium dioxide, and identified with nano‐LC‐MS/MS using an automatic cross‐validation of MS/MS and MS/MS/MS (MS2+MS3) data‐dependent neutral loss method. A spectral counting quantitative strategy was applied to the two cell line samples on the MS2‐only scan, which was implemented successively after each MS2+MS3 scan in the same sample. For all regulated phosphopeptides reported by spectral counting analysis, sequence and phosphorylation site assignments were validated by a MS2+MS3 data‐dependent neutral loss method. With this approach, we identified over 70 phosphorylated sites on 27 phosphoproteins as being differentially expressed with respect to tumor cell phenotype. The altered expression levels of proteins identified by LC‐MS/MS were validated using Western blotting. Using network pathway analysis, we observed that the majority of the differentially expressed proteins were highly interconnected and belong to two major intracellular signaling pathways. Our findings suggest that the phosphorylation of isoform A of lamin A/C and GTPase activating protein binding protein 1 is associated with metastatic propensity. The study demonstrates a quantitative and comparative proteomics strategy to identify differential phosphorylation patterns in complex biological samples.
A quantitative investigation of fucosylated serum glycoproteins with application to esophageal adenocarcinoma
Authors: Mann Benjamin , Madera Milan , Klouckova Iveta , Mechref Yehia , Dobrolecki Lacey E. , Hickey Robert J. , Hammoud Zane T. , Novotny Milos V. 
Published: May 2010

AbstractAlthough glycoproteomic studies provide unique opportunities for cancer research, it has been necessary to develop specific methods for analysis of oncologically interesting glycoproteins. We describe a general, multimethodological approach for quantitative glycoproteomic analysis of fucosylated glycoproteins in human blood serum. A total of 136 putative fucosylated glycoproteins were identified with very high confidence in three clinically relevant sample pools (N=5 for each), with a mean CV of 3.1% observed for replicate analyses. Two samples were collected from subjects diagnosed with esophagus disease states, high‐grade dysplasia plus esophageal adenocarcinoma, while the third sample was representative of a disease‐free condition. Some glycoproteins, observed to be significantly upregulated in esophageal adenocarcinoma, i.e. more than twofold higher than in the disease‐free condition, are briefly discussed. Further investigation will be necessary to validate these findings; however, the method itself is demonstrated to be an effective tool for quantitative glycoproteomics of clinical samples.
High‐order gaussian quadrature in X‐FEM with the lagrange‐multiplier for fluid–structure coupling
Authors: Sawada Tomohiro , Tezuka Akira 
Published: May 2010

AbstractThis paper presents an alternative choice for the construction of integration cells and an additional Lagrange‐multiplier mesh in computations using the eXtended finite element method (X‐FEM) with the Lagrange‐multiplier. The proposed computational target is flow simulations with the Dirichlet boundary conditions on a non‐boundary‐fitted mesh. The methods studied in this paper make use of a high‐order Gaussian quadrature to relax the complexity in implementing the X‐FEM with the Lagrange‐multiplier. The first of these is the straightforward employment of the Gaussian quadrature as a numerical integration scheme for enriched fluid elements. The second makes use of free Lagrange‐multipliers that are not placed at crossing points between fluid elements and the Dirichlet boundary. This method considers the scalability of fluid–structure and fluid–thin object interactions. A unique benchmark problem in which a computational fluid domain is partitioned into two physical domains with the Lagrange‐multipliers is introduced to validate the proposed method. Numerical results show that this method is applicable in practice with acceptable numerical accuracy. Copyright © 2010 John Wiley & Sons, Ltd.
Alteration of glial‐neuronal metabolic interactions in a mouse model of Alexander disease
Authors: Meisingset Tore Wergeland , Risa Øystein , Brenner Michael , Messing Albee , Sonnewald Ursula 
Published: May 2010

AbstractAlexander disease is a rare and usually fatal neurological disorder characterized by the abundant presence of protein aggregates in astrocytes. Most cases result from dominant missense de novo mutations in the gene encoding glial fibrillary acidic protein (GFAP), but how these mutations lead to aggregate formation and compromise function is not known. A transgenic mouse line (Tg73.7) over‐expressing human GFAP produces astrocytic aggregates indistinguishable from those seen in the human disease, making them a model of this disorder. To investigate possible metabolic changes associated with Alexander disease Tg73.7 mice and controls were injected simultaneously with [1‐13C]glucose to analyze neuronal metabolism and [1,2‐13C]acetate to monitor astrocytic metabolism. Brain extracts were analyzed by 1H magnetic resonance spectroscopy (MRS) to quantify amounts of several key metabolites, and by 13C MRS to analyze amino acid neurotransmitter metabolism. In the cerebral cortex, reduced utilization of [1,2‐13C]acetate was observed for synthesis of glutamine, glutamate, and GABA, and the concentration of the marker for neuronal mitochondrial metabolism, N‐acetylaspartate (NAA) was decreased. This indicates impaired astrocytic and neuronal metabolism and decreased transfer of glutamine from astrocytes to neurons compared with control mice. In the cerebellum, glutamine and GABA content and labeling from [1‐13C]glucose were increased. Evidence for brain edema was found in the increased amount of water and of the osmoregulators myo‐inositol and taurine. It can be concluded that astrocyte—neuronal interactions were altered differently in distinct regions. © 2010 Wiley‐Liss, Inc.
Reproductive and hormonal factors and the risk of nonsmall cell lung cancer
Authors: Meinhold Cari L. , Berrington de González Amy , Bowman Elise D. , Brenner Alina V. , Jones Raymond T. , Lacey James V. , Loffredo Christopher A. , Perlmutter Donna , Schonfeld Sara J. , Trivers Glenwood E. , Harris Curtis C. 
Published: May 2010

AbstractAlthough exposure to estrogen may directly influence or modify the association between cigarette smoking and lung cancer risk, results from epidemiologic studies examining the association between reproductive and hormonal factors and risk of lung cancer among women have been inconsistent. Between 1998 and 2008, 430 women diagnosed with nonsmall cell lung cancer, 316 hospital controls and 295 population controls were recruited into the multi‐center Maryland Lung Cancer Study. Conditional logistic regression was used to estimate odds ratios (ORs) and 95% confidence intervals (CIs) according to reproductive and hormonal exposures adjusting for age, smoking, passive smoking, education and household income. Results were similar for hospital and population based controls, so the control groups were combined. Reduced risks of lung cancer were observed among women with greater parity (≥5 vs. 1–2 births: OR = 0.50, 95% CI = 0.32, 0.78, p‐trend = 0.002) and later ages at last birth (≥30 vs. <25 years old: OR = 0.68, 95% CI = 0.48, 0.98, p‐trend = 0.04). After mutual adjustment parity, but not age at last birth, remained significantly inversely associated with risk (p‐trend = 0.01). No associations were found for nonsmall cell lung cancer risk with age at menarche, age at first birth, menopausal status, oral contraceptive use or menopausal hormone use, including use of oral estrogens. Compatible with findings from recent epidemiologic studies, we observed a reduction in the risk of nonsmall cell lung cancer with increasing number of births. Other reproductive and hormonal exposures, including menopausal hormone therapy use, were not associated with risk.
Accurate prediction of neuroblastoma outcome based on miRNA expression profiles
Authors: Schulte Johannes H. , Schowe Benjamin , Mestdagh Pieter , Kaderali Lars , Kalaghatgi Prabhav , Schlierf Stefanie , Vermeulen Joelle , Brockmeyer Bent , Pajtler Kristian , Thor Theresa , de Preter Katleen , Speleman Frank , Morik Katharina , Eggert Angelika , Vandesompele Jo , Schramm Alexander 
Published: May 2010

AbstractFor neuroblastoma, the most common extracranial tumour of childhood, identification of new biomarkers and potential therapeutic targets is mandatory to improve risk stratification and survival rates. MicroRNAs are deregulated in most cancers, including neuroblastoma. In this study, we analysed 430 miRNAs in 69 neuroblastomas by stem‐loop RT‐qPCR. Prediction of event‐free survival (EFS) with support vector machines (SVM) and actual survival times with Cox regression‐based models (CASPAR) were highly accurate and were independently validated. SVM‐accuracy for prediction of EFS was 88.7% (95% CI: 88.5–88.8%). For CASPAR‐based predictions, 5y‐EFS probability was 0.19% (95% CI: 0–38%) in the CASPAR‐predicted short survival group compared with 0.78% (95%CI: 64–93%) in the CASPAR‐predicted long survival group. Both classifiers were validated on an independent test set yielding accuracies of 94.74% (SVM) and 5y‐EFS probabilities as 0.25 (95% CI: 0.0–0.55) for short versus 1 ± 0.0 for long survival (CASPAR), respectively. Amplification of the MYCN oncogene was highly correlated with deregulation of miRNA expression. In addition, 37 miRNAs correlated with TrkA expression, a marker of excellent outcome, and 6 miRNAs further analysed in vitro were regulated upon TrkA transfection, suggesting a functional relationship. Expression of the most significant TrkA‐correlated miRNA, miR‐542‐5p, also discriminated between local and metastatic disease and was inversely correlated with MYCN amplification and event‐free survival. We conclude that neuroblastoma patient outcome prediction using miRNA expression is feasible and effective. Studies testing miRNA‐based predictors in comparison to and in combination with mRNA and aCGH information should be initiated. Specific miRNAs (e.g., miR‐542‐5p) might be important in neuroblastoma tumour biology, and qualify as potential therapeutic targets.
The Haven Mother's House Modified Therapeutic Community: Meeting the gap in infant mental health services for pregnant and parenting mothers with drug addiction
Authors: Bromberg Stacey R. , Backman Toni L. , Krow Julie , Frankel Karen A. 
Published: May 2010

AbstractThe specialized needs of pregnant and parenting women in the treatment of drug addiction must not be underestimated. The impact of substance abuse on developmental outcomes for young infants and children supports the notion that attention to the parent–child relationship is a critical aspect of addiction treatment for this population. As such, the standard of care appears to be shifting from separating mothers and young children while the mother completes addiction treatment to women residing with their children while in treatment and receiving concurrent addiction treatment and parenting education. While parenting education is important, it may not provide the needed relationship intervention to address the myriad of issues often present for female recovering addicts and their children. This article describes the evolution and workings of a program for integrating infant mental health practice into a long‐term residential treatment community for pregnant and parenting women with addiction. The principles and structure of the modified therapeutic community are described, as are the ways in which infant mental health practice have been effectively integrated and incorporated into the addiction treatment philosophy. A case example is provided, and clinical implications are discussed.
The interface of mental and emotional health and pregnancy in urban indigenous women: Research in progress
Authors: Hayes Barbara A. , Campbell Alistair , Buckby Beryl , Geia Lynore K. , Egan Margaret E. 
Published: May 2010

AbstractResearch among indigenous women in Australia has shown that a number of lifestyle factors are associated with poor obstetric outcomes; however, little evidence appears in the literature about the role of social stressors and mental health among indigenous women. The not‐for‐profit organization beyondblue established a “Depression Initiative” in Australia. As part of this they provided funding to the Townsville Aboriginal and Torres Strait Islander Health Service in the “Mums and Babies” clinic. The aim of this was to establish a project to (a) describe the mental health and level of social stressors among antenatal indigenous women and (b) assess the impact of social stressors and mental health on perinatal outcome. A purposive sample of 92 indigenous women was carried out. Culturally appropriate research instruments were developed through consultations with indigenous women's reference groups. The participants reported a range of psychosocial stressors during the pregnancy or within the last 12 months. Significant, positive correlations emerged between the participants' Edinburgh Postnatal Depression Scale (EPDS; J. Cox, J. Holden, & R. Sagovsky, 1987) score and the mothers' history of child abuse and a history of exposure to domestic violence. A more conservative cutoff point for the EPDS (>9 vs. >12) led to 28 versus 17% of women being identified as “at risk” for depression. Maternal depression and stress during pregnancy and early parenthood are now recognized as having multiple negative sequelae for the fetus and infant, especially in early brain development and self‐regulation of stress and emotions. Because of the cumulative cultural losses experienced by Australian indigenous women, there is a reduced buffer to psychosocial stressors during pregnancy; thus, it is important for health professionals to monitor the women's emotional and mental well‐being.
Improving irrigation management in dry season rice cultivation for optimum crop and water productivity in non‐traditional rice ecologies
Authors: Ghosh A. , Singh O. N. , Rao K. S. 
Published: May 2010

AbstractA field experiment carried out during 2003 and 2004 in India addressed critical soil moisture tension based alternate wetting and drying (AWD) system of irrigation management in dry season rice cultivation in non‐traditional areas under waterlogged and flood‐prone deepwater conditions. Results showed that a medium duration rice variety, “Geetanjali”, grown with irrigation up to 0.1 bar soil moisture tension produced a comparable grain yield of 4.30–4.50 t ha−1 with that under conventional irrigation management (4.50–4.75 t ha−1). However, a significant yield decline of 17.8–18.1% occurred while applying irrigation at 0.2 bar moisture tension. Mean irrigation water requirement was a maximum of 2295 mm in the conventional system; 28.0, 41.7 and 55.8% higher than those at 0.0, 0.1 and 0.2 bar tension, respectively. Average water use efficiency of 3.57 kg grain ha‐mm−1 was also significantly higher at 0.2 bar tension. Irrigation at 0.1 bar moisture tension achieved optimum crop productivity of 4.3–4.4 t ha−1 and water productivity of 0.32–0.34 g grain kg−1 water applied.Thus, it is expected that the results of this study would become very useful while planning future research programmes on water‐saving irrigation management, viz. the AWD system in rice cultivation. Copyright © 2010 John Wiley & Sons, Ltd.
Plasmapheresis in the treatment of hypertriglyceridemia‐induced pancreatitis: A community hospital's experience
Authors: Syed H. , Bilusic M. , Rhondla C. , Tavaria A. 
Published: May 2010

AbstractBackground: Hyperlipidemic pancreatitis is a potentially fatal complication of hypertriglyceridemia (HTG). The current mainstay of treatment for the hypertriglyceridemia associated with pancreatitis includes heparin, insulin and lipid lowering agents. Experiences with plasmapheresis are limited. Here, we report our experience using plasmapheresis in the treatment of four patients with acute severe HTG‐induced pancreatitis. Methods: Four patients with acute severe HTG‐induced pancreatitis due to severe primary hyperlipidemia exacerbated by secondary factors were studied. In addition to the standard treatment (insulin or heparin infusion), antibiotics and lipid lowering agents, two were treated within the first 48 hours, and two with early (<24 hours) plasmapheresis with 5% albumin. Results: All four patients had a significant improvement in their triglyceride levels using plasmapheresis with an average reduction in TG levels of 70.4% per treatment and 89.3% with the first treatment. However, there was no clear relation between the use of plasmapheresis and either improvement in APACHE II scores, length of stay in either the ICU or overall hospital length of stay or in the prevention of complications secondary to severe pancreatitis, including in the two patients who received plasmapheresis in the first 24 hours. Conclusion: Our report showed that plasmapheresis was successful in lowering TG levels. However, in the absence of a comparison with standard treatment (heparin or insulin infusion and lipid lowering agents) the effect of plasmapheresis on lowering the morbidity and length of stay of patients with HTG‐induced acute severe pancreatitis is uncertain and warrants further investigation into its efficacy. J. Clin. Apheresis 25:229–234, 2010. © 2010 Wiley‐Liss, Inc.
Granulocyte colony‐stimulating factor produces a decrease in IFNγ and increase in IL‐4 when administrated to healthy donors
Authors: Rodríguez‐Cortés Octavio , Vela‐Ojeda Jorge , López‐Santiago Rubén , Montiel‐Cervantes Laura , Reyes‐Maldonado Elba , Estrada‐García Iris , Moreno‐Lafont Martha C. 
Published: May 2010

AbstractHematopoietic stem cells transplantation (HSCT) is the leading curative therapy for a variety of hematological and hereditary diseases; however, graft versus host disease (GVHD), an immunologic phenomenon that is favored by Th1 cytokines and cytotoxic cells from donors, is present frequently and is one of the most important causes of transplant related mortality. Peripheral blood HSCT is the preferred source of stem cells in almost 100% of the cases of autologous HSCT and in 70% of allogeneic transplants. The best mobilizing agent to get the stem cells out from the bone marrow is the Granulocyte‐Colony Stimulating Factor (G‐CSF). In this work, our main objective was to study a possible correlation between the graft cell dose and the patient's clinical outcome. We evaluated the immunologic changes produced by G‐CSF in the lymphocyte and cytokine profiles in allogeneic HSC donors. HSC from twelve donors were mobilized with G‐CSF at 16 μg/kg/day, for 5 days. Basal Peripheral Blood (BPB), Mobilized Peripheral Blood (MPB), and aphaeresis mononuclear cells (G‐MNC) samples were taken from all donors. Using flow cytometry, we quantified CD19+, CD3+, CD3+CD4+, CD3+CD8+, NK, NKT, DC1, and DC2 cells. Cytokines were determined by ELISA in culture supernatants. CD19+ (p = 0.001), DC1 (p < 0.002) and DC2 (p < 0.001) cells were increased in MPB with respect to BPB. An increase in Th2 cytokines such as (IL‐4) and a decrease in Th1 cytokines (IFNγ, IL‐2) were also found in MPB samples. In conclusion, Th1 and Th2 cytokines are relevant in predicting the clinical outcome after allogeneic peripheral blood HSCT. J. Clin. Apheresis 25:181–187, 2010. © 2010 Wiley‐Liss, Inc.
Increased risk of citrate reactions in patients with multiple myeloma during peripheral blood stem cell leukapheresis
Authors: Adamski Jill , Griffin Adrienne Carruth , Eisenmann Christa , Milone Michael C. , Sachais Bruce S. 
Published: May 2010

AbstractThe citrate based anticoagulant ACD is commonly used in apheresis procedures. Due to its ability to decrease ionized calcium, citrate may cause unpleasant symptoms, such as paresthesias and muscle cramps, in patients undergoing therapeutic and donor apheresis. We noticed that patients with multiple myeloma (MM) undergoing autologous stem cell leukapheresis appeared to have more citrate reactions when compared to other patients undergoing the same procedure. A retrospective chart review was performed to evaluate 139 (of 151) consecutive patients with MM, amyloidosis, hematological and solid malignancies who had autologous peripheral blood stem cell collection between January 2007 and February 2008. Citrate reactions, ranging from mild (e.g., perioral tingling and parasthesias) to severe (e.g., nausea/vomiting and muscle cramps) were noted for 35 patients. Twenty‐three of 63 patients with MM had documented citrate reactions, which was significantly higher than those with other hematological and solid malignancies (37% vs. 20%; P < 0.05, Relative Risk (RR) = 1.9). The severities of citrate reactions were the same in both groups; approximately 50% of patients in each group received i.v. calcium gluconate for treatment of hypocalcemia. No correlation between bisphosphonate therapy and citrate reactions were noted in our study group. Examination of available laboratory values related to calcium homeostasis, liver, and renal function failed to reveal a mechanism for the increase in citrate reactions observed. In summary, this single institution retrospective study indicates that patients with MM are more sensitive to citrate‐induced hypocalcemia during leukapheresis when compared to patients with other hematological and solid malignancies. Strategies for decreasing citrate reactions (e.g., supplemental calcium and slowing return rates) should be considered for patient safety and comfort, especially in the MM population, on a prophylactic rather than reactive basis. J. Clin. Apheresis 25:188–194, 2010. © 2010 Wiley‐Liss, Inc.
Mutual interaction and reciprocal down‐regulation between c‐met and insulin receptor substrate‐1
Authors: DeAngelis Tiziana , Morrione Andrea , Baserga Renato 
Published: May 2010

AbstractThe insulin receptor substrate‐1 (IRS‐1) and c‐met, the receptor for the hepatocyte growth factor (HGF) co‐immuno‐precipitate from lysates treated with the respective antibodies. The interaction between IRS‐1 and c‐met requires a tyrosyl phosphorylated IRS‐1 and results in reciprocal down‐regulation. IRS‐1 inhibits cell motility, while the activated c‐met promotes it. These and other results suggest an explanation for reports in the literature indicating that c‐met levels are high and IRS‐1 levels are low in human cancer metastases. J. Cell. Physiol. 224: 658–663, 2010. © 2010 Wiley‐Liss, Inc.