bluuebunny/crossref_metadata_2025_split huggingface dataset:
Unexpected roles for DEAD‐box protein 3 in viral RNA sensing pathways
Authors: Mulhern Orla , Bowie Andrew G. 
Published: March 2010

AbstractDetection of viral nucleic acid within infected cells is essential to an effective anti‐viral response. The retinoic acid‐inducible gene‐I‐like receptors (RLR) form part of the virus detection repertoire and are critically important in sensing viral RNA in the cytoplasm. Efforts continue to define the signalling components downstream of RLR that are required to induce type I IFN (IFN‐α and promoter stimulator‐1) after viral infection. One surprising finding was that the Asp‐Glu‐Ala‐Asp box helicase DEAD/H Box 3 (DDX3), known for some time to have a number of roles in cellular RNA regulation in the nucleus, has a role in the RLR cytoplasmic signalling pathway involved in promoter stimulator‐1 induction. In this issue of theEuropean Journal of Immunology, an article reports an additional distinct positive role for DDX3 in the RLR RNA sensing pathway. This further emphasises the importance of DDX3 in anti‐viral immunity, and is consistent with the idea that viruses target DDX3 for immune evasion.
Bystander activation of CD4+ T cells
Authors: Boyman Onur 
Published: March 2010

AbstractBystander activation of T cells, i.e. the stimulation of unrelated (heterologous) T cells by cytokines during an Ag‐specific T‐cell response, has been best described for CD8+ T cells. In the CD8+ compartment, the release of IFN and IFN‐inducers leads to the production of IL‐15, which mediates the proliferation of CD8+ T cells, notably memory‐phenotype CD8+ T cells. CD4+ T cells also undergo bystander activation, however, the signals inducing this Ag‐nonspecific stimulation of CD4+ T cells are less well known. A study in this issue of the European Journal of Immunology sheds light on this aspect, suggesting that common γ‐chain cytokines including IL‐2 might be involved in bystander activation of CD4+ T cells.
Photoactivated Biological Activity of Transition‐Metal Complexes (Eur. J. Inorg. Chem. 10/2010)
Authors: Schatzschneider Ulrich 
Published: March 2010

AbstractThe cover picture shows examples of metal complexes with photoactivated biological activity, as symbolized by the lightning in the background. This includes liberation of small‐molecule messengers, such as carbon monoxide, nitric oxide, and neuroactive amines from the metal coordination sphere, as well as light‐triggered oxidative or covalent modification of DNA. More examples are presented in the Microreview by U. Schatzschneider on p. 1451 ff.
Study of elution behaviour with gradient voltage in CEC using methacrylate monolithic columns
Authors: Baeza‐Baeza Juan José , Bernabé‐Zafón Virginia , Herrero‐Martínez José Manuel , Simó‐Alfonso Ernesto F. 
Published: March 2010

AbstractA theoretical study on the retention behaviour and chromatographic performance of neutral solutes using a lauryl methacrylate‐based monolithic column under voltage gradient mode in CEC was carried out. Through a flexible mathematical function based on a modified Gaussian model, the peak shape of compounds was firstly fitted under constant and gradient voltage. Using the peak shape parameters and retention time, the estimation of global chromatographic performance, efficiency and peak capacity under several voltage conditions was performed. The influence of voltage gradient on the separation efficiency is discussed and simple equations are presented to calculate retention and peak widths under voltage gradient conditions. A comparison in terms of chromatographic performance of a test mixture of neutral solutes under constant and gradient voltage modes was also carried out. The experiments carried out under gradient voltage showed better efficiencies (172 000 plates/m) and lower peak widths than those obtained under constant voltage (52 000 plates/m).
Glycogen: A novel branched polysaccharide chiral selector in CE
Authors: Chen Jiaquan , Du Yingxiang , Zhu Fenxia , Chen Bin 
Published: March 2010

AbstractVarious chiral selectors have been employed in CE and among them linear polysaccharides exhibited powerful enantioselective properties. Different from linear polysaccharides, the use of branched polysaccharides as chiral selectors in CE has not been reported previously. In this study glycogen belonging to the class of branched polysaccharides was used as a novel chiral selector for the enantiomeric separations for the first time. Since glycogen is electrically neutral, the method is applicable to ionic compounds. Eighteen chiral compounds including 12 basic drugs and six acidic drugs have been tested to demonstrate the potential of this chiral selector. BGE and selector concentrations and buffer pH were systematically optimized in order to obtain successful chiral separations. Among the tested compounds, the enantiomers of ibuprofen, which is an acidic drug, were successfully recognized by 3.0% w/v glycogen with 90 mM Tris‐H3PO4 buffer (pH 7.0). The enantiomers of basic drugs such as citalopram, cetirizine and nefopam were also baseline‐resolved with 50 mM Tris‐H3PO4 buffer (pH 3.0) containing 3.0% glycogen. Amlodipine belonging to basic compound only gave partial enantioseparation under the above‐mentioned condition.
Heart‐cutting 2D‐CE with on‐line preconcentration for the chiral analysis of native amino acids
Authors: Anouti Suzanne , Vandenabeele‐Trambouze Odile , Cottet Hervé 
Published: March 2010

AbstractThe use of transient moving chemical reaction boundary (tMCRB) was investigated for the on‐line preconcentration of native amino acids in heart‐cutting 2D‐CE with multiple detection points using contactless conductivity detection. The tMCRB focusing was obtained by using ammonium formate (pH 8.56) as sample matrix and acetic acid (pH 2.3) as a BGE in the first dimension of the heart‐cutting 2D‐CE. Different experimental parameters such as the injected volume and the concentration in ammonium formate were optimized for improving the sensitivity of detection. A stacked fraction from the first dimension was selected, isolated in the capillary, and then separated in the second dimension in the presence of a chiral selector ((+)‐(18‐crown‐6)‐2,3,11,12‐tetracarboxylic acid). This on‐line tMCRB preconcentration coupled with heart‐cutting 2D‐CE was applied with success to the chiral separation of D,L‐phenylalanine, and D,L‐threonine in a mixture of 22 native amino acids. The sample mixture was diluted in 0.8 M of ammonium formate, and injected at a concentration of 2.5 μM for each enantiomer with a volume corresponding to 10% of the total capillary volume. An LOD (S/N=3) of 2 μM was determined for L‐threonine.
Microchip electrophoresis of Alu elements for gender determination and inference of human ethnic origin
Authors: Njoroge Samuel K. , Witek Małgorzata A. , Hupert Mateusz L. , Soper Steven A. 
Published: March 2010

AbstractWe performed a series of multi‐locus PCRs followed by the rapid and efficient microchip electrophoretic sorting of Alu products with LIF detection. Five polymorphic human‐specific Alu insertions (RC5, A1, PV92, TPA and ACE) were used for inference of human ethnicity and two monomorphic Alu insertions for sex typing, one fixed on the X chromosome (AluSTXa) and the other on the Y chromosome (AluSTYa). These markers were used to generate unique DNA profiles for five different DNA samples. The PCR‐based assays used primers that flank the insertion point to determine genotypes based on the presence or absence of the Alu element. A1, RC5, PV92, TPA and ACE were used for ethnicity determinations and have two alleles, each indicating the presence (+) or absence (−) of the Alu element on the paired chromosomes, which results in three genotypes (+/+, +/− or −/−). RC5 and A1 did not show ethnic heterogeneity resulting in a homozygous (−/−) genotype, which correctly inferred that DNA samples originating from a Caucasian male and an Asian male were not of African ancestry. The results from the five Alu markers indicated that these Alu loci could assist in identifying the individual's ethnicity using microchip electrophoresis in under 15 min of separation time. Using microchip electrophoresis and mixed genotype ratios, male DNA‐to‐female DNA of 1:9, corresponding to a ratio of Y‐to‐X chromosomes of 1:19, was also detected for both AluSTXa and AluSTYa to provide gender identification without requiring separation of female from male cells prior to the assay.
Triblock copolymer matrix‐based capillary electrophoretic microdevice for high‐resolution multiplex pathogen detection
Authors: Kim Se Jin , Shin Gi Won , Choi Seok Jin , Hwang Hee Sung , Jung Gyoo Yeol , Seo Tae Seok 
Published: March 2010

AbstractRapid and simple analysis for the multiple target pathogens is critical for patient management. CE‐SSCP analysis on a microchip provides high speed, high sensitivity, and a portable genetic analysis platform in molecular diagnostic fields. The capability of separating ssDNA molecules in a capillary electrophoretic microchannel with high resolution is a critical issue to perform the precise interpretation in the electropherogram. In this study, we explored the potential of poly(ethyleneoxide)‐poly(propyleneoxide)‐poly(ethyleneoxide) (PEO‐PPO‐PEO) triblock copolymer as a sieving matrix for CE‐SSCP analysis on a microdevice. To demonstrate the superior resolving power of PEO‐PPO‐PEO copolymers, 255‐bp PCR amplicons obtained from 16S ribosomal RNA genes of four bacterial species, namely Proteus mirabilis, Haemophilus ducreyi, Pseudomonas aeruginosa, and Neisseria meningitidis, were analyzed in the PEO‐PPO‐PEO matrix in comparison with 5% linear polyacrylamide and commercial GeneScan™ gel. Due to enhanced dynamic coating and sieving ability, PEO‐PPO‐PEO copolymer displayed fourfold enhancement of resolving power in the CE‐SSCP to separate same‐sized DNA molecules. Fivefold input of genomic DNA of P. aeruginosa and/or N. meningitidis produced proportionally increased corresponding amplicon peaks, enabling correct quantitative analysis in the pathogen detection. Besides the high‐resolution sieving capability, a facile loading and replenishment of gel in the microchannel due to thermally reversible gelation property makes PEO‐PPO‐PEO triblock copolymer an excellent matrix in the CE‐SSCP analysis on the microdevice.
Simultaneous electrophoretic analysis of proteins of very high and low molecular mass using Tris‐acetate polyacrylamide gels
Authors: Cubillos‐Rojas Monica , Amair‐Pinedo Fabiola , Tato Irantzu , Bartrons Ramon , Ventura Francesc , Rosa Jose Luis 
Published: March 2010

AbstractTo separate and analyze giant and small proteins in the same electrophoresis gel, we have used a 3–15% polyacrylamide gradient gel containing 2.6% of the crosslinker bisacrylamide and 0.2 M of Tris‐acetate buffer (pH 7.0). Samples were prepared in a sample buffer containing lithium dodecyl sulphate and were run in the gel described above using Tris‐Tricine‐SDS‐sodium bisulfite buffer, pH 8.2, as electrophoresis buffer. Here, we show that this system can be successfully used for general applications of SDS‐PAGE such as CBB staining and immunoblot. Thus, by using Tris‐acetate 3–15% polyacrylamide gels, it is possible to simultaneously analyze proteins, in the mass range of 10–500 kDa, such as HERC1 (532 kDa), HERC2 (528 kDa), mTOR (289 kDa), Clathrin heavy chain (192 kDa), RSK (90 kDa), S6K (70 kDa), β‐actin (42 kDa), Ran (24 kDa) and LC3 (18 kDa). This system is highly sensitive since it allows detection from as low as 10 μg of total protein per lane. Moreover, it has a good resolution, low cost, high reproducibility and allows for analysis of proteins in a wide range of weights within a short period of time. All these features together with the use of a standard electrophoresis apparatus make the Tris‐acetate‐PAGE system a very helpful tool for protein analysis.
Simplified analysis of mid‐story seismically isolated buildings
Authors: Wang Shiang‐Jung , Chang Kuo‐Chun , Hwang Jenn‐Shin , Lee Bo‐Han 
Published: March 2010

AbstractThe mid‐story isolation design method is recently gaining popularity for the seismic protective design of buildings located in the areas of high population. In a mid‐story isolated building, the isolation system is incorporated into the mid‐story rather than the base of the building. In this paper, the dynamic characteristics and seismic responses of mid‐story isolated buildings are investigated using a simplified three‐lumped‐mass structural model for which equivalent linear properties are formulated. From the parametric study, it is found that the nominal frequencies of the superstructure and the substructure, respectively, above and below the isolation system have significant influences on the isolation frequency and equivalent damping ratio of a mid‐story isolated building. Moreover, the mass and stiffness of the substructure are of greater significance than the superstructure in affecting the dynamic characteristics of the isolated building. Besides, based on the response spectrum analysis, it is noted that the higher mode responses may contribute significantly to the story shear force of the substructure. Consequently, the equivalent lateral force procedure of design codes should carefully include the effects of higher modes. Copyright © 2010 John Wiley & Sons, Ltd.
A facile route to 1,5‐benzodiazepin‐2‐yl‐phosphonates via ytterbium chloride‐catalyzed three‐component reaction
Authors: Ji Xiaofeng , Cai Lijian , Yao Zhigang , Fan Xu , Qi Shen 
Published: March 2010

Abstract1,5‐Benzodiazepin‐2‐yl‐phosphonates were facilely synthesized via a one‐pot three‐component condensation of o‐diaminobenzene, 1,3‐diketone and diethyl phosphite in the presence of a catalytic amount of ytterbium chloride under mild reaction conditions. © 2010 Wiley Periodicals, Inc. Heteroatom Chem 21:89–95, 2010; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/hc.20573
One‐pot synthesis of α‐aminophosphonates catalyzed by boric acid at room temperature
Authors: Karimi‐Jaberi Zahed , Amiri Mohammad 
Published: March 2010

AbstractA simple, efficient, and general method has been developed for the one‐pot, three‐component synthesis of α‐aminophosphonates from a condensation reaction of trimethyl phosphite, aldehydes, and amines in the presence of catalytic amount of boric acid (10 mol%) under solvent‐free conditions. Thus α‐aminophosphonates were synthesized relatively quickly in good yields at room temperature. © 2010 Wiley Periodicals, Inc. Heteroatom Chem 21:96–98, 2010; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/hc.20577
A new family of platinum(II) complexes incorporating five‐ and six‐membered cyclic phosphine ligands
Authors: Kerényi Andrea , Kovács Viktória , Körtvélyesi Tamás , Ludányi Krisztina , Drahos László , Keglevich György 
Published: March 2010

AbstractNew platinum complexes of the type cis‐Pt(L)2Cl2 have been synthesized from five‐ and six‐membered cyclic phosphines, which were prepared after deoxygenating a series of phosphine oxides (3‐phospholene oxides, phospholane oxides, a 1,4‐dihydrophosphinine oxide, and a 1,2,3,6‐tetrahydrophosphinine oxide). The complexes were characterized by NMR and mass spectral data, and their stereostructures were elucidated by B3LYP/6‐31G(d)‐LANL2DZ ECP calculations. The phosphine intermediates were characterized as the corresponding phosphine‐boranes. © 2010 Wiley Periodicals, Inc. Heteroatom Chem 21:63–70, 2010; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/hc.20579
Synthesis of biologically active phenoxy derivatives of substituted benzothiazole organophosphates
Authors: Jhajharia Priyanka , Samota Mahesh Kumar , Seth Gita 
Published: March 2010

AbstractThe reaction of S‐(phenyl benzothiazolyl‐2)phosphorodichloridothioate/phosphorodichloridodithioate with 2 mol of phenol/4‐chlorophenol/4‐nitrophenol in the presence of stoichiometric amounts of triethylamine in dry THF/CH2Cl2 has afforded a series of the corresponding organophosphate phenoxy derivatives (1a, 1b, 2a, 2b, and 3a, 3b). Plausible structures have been proposed on the basis of elemental analysis, IR, 1H NMR, 31P NMR, and mass spectral studies. The antibacterial activity of these organophosphate phenoxy derivatives has been evaluated against pathogenic bacteria Staphylococcus aureus (+ve) and Escherichia coli (−ve). The antifungal activity of these organophosphate phenoxy derivatives has been evaluated against pathogenic fungi Aspergillus niger and Fusarium oxysporium. The results indicate that organophosphate phenoxy derivatives are found more active than the parent compounds. © 2010 Wiley Periodicals, Inc. Heteroatom Chem 21:84–88, 2010; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/hc.20582
Hydroxyapatite versus titanium implant: Comparison of the functional outcome after vocal fold medialization in unilateral recurrent nerve paralysis
Authors: Storck Claudio , Fischer Claude , Cecon Mikis , Schmid Stephan , Gambazzi Franco , Wolfensberger Markus , Brockmann Meike 
Published: March 2010

AbstractBackground.Results of medialization thyroplasty for treatment of unilateral vocal fold paralysis are often unsatisfactory. This study compares glottal closure and voice quality after use of 2 different medialization implant types: VoCoM and TVFMI.Methods.In all, 26 patients with unilateral vocal fold paralysis following thoracic surgery underwent medialization thyroplasty. In 11 patients (group I), a hydroxyapatite implant (VoCoM) was used. In 15 patients (group II), a titanium implant (TVFMI) was used. Preoperative and postoperative glottal closure and voice function were assessed with videostroboscopy, perceptual and objective voice measures, and the Voice Dysfunction Index (VDI).Results.Group II showed a higher rate of complete glottal closure and greater improvement in perceived hoarseness, maximal phonation time, and maximal voice intensity than those in group I.Conclusions.With the individually adjustable titanium implant, better glottal closure and better functional outcome (phonation time and voice quality) were achieved. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Pseudoaneurysm of the lingual artery after concurrent intra‐arterial chemotherapy with radiotherapy for advanced tongue cancer
Authors: Murono Shigeyuki , Nakanishi Yosuke , Inoue Dai , Ozaki Kumi , Kondo Satoru , Wakisaka Naohiro , Matsui Osamu , Yoshizaki Tomokazu 
Published: March 2010

AbstractBackground.Aneurysms of the external carotid artery and its branches are rare.Methods.We present an extremely rare case of pseudoaneurysm of the lingual artery secondary to concurrent intra‐arterial chemotherapy via the superficial temporal artery for advanced tongue cancer.Results.The patient developed sudden and significant oral bleeding from the left tongue base 10 weeks after the completion of treatment. Angiography provided a diagnosis of pseudoaneurysm of the lingual artery, and subsequent endovascular intervention was an effective means of controlling bleeding.Conclusion.Rupture of a pseudoaneurysm causes massive, life‐threatening bleeding, which requires adequate diagnosis and treatment. Pseudoaneurysm should be considered in cases showing sudden oral bleeding after intra‐arterial chemotherapy for advanced head and neck cancer. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Parathyroid carcinoma: A review
Authors: Owen Randall P. , Silver Carl E. , Pellitteri Phillip K. , Shaha Ashok R. , Devaney Kenneth O. , Werner Jochen A. , Rinaldo Alessandra , Ferlito Alfio 
Published: March 2010

AbstractBackground.Parathyroid carcinoma is a rare entity, comprising fewer than 1% of cases of hyperparathyroidism. The disease generally presents with severe hyperparathyroidism and occasionally with vocal cord paralysis or a firm palpable cervical mass.Methods.The classic histopathologic features of trabecular pattern, mitotic figures, thick fibrous bands, and capsular and vascular invasion are not present in every case but are useful in assessing whether a particular hyperfunctional parathyroid lesion is a carcinoma. Nevertheless, recognition of malignancy at initial operation may be difficult. Management primarily involves complete surgical removal through en bloc resection, which may include adjacent central neck structures.Results.Adjuvant radiation therapy has not been proven to be uniformly effective, but several series show results suggestive of a possible survival advantage. Chemotherapy, genetic, and other biomodifying agents remain experimental. Long‐term outcome for this neoplasm remains problematic, and complications from intractable hypercalcemia constitute the foremost cause of death. Reported 5‐year survivals have ranged between 70% and 85%.Conclusions.Initial surgical success offers the greatest opportunity for cure. Reoperation for recurrence offers the potential for short‐ and intermediate‐term relief from the sequelae of hypercalcemia, but rarely results in cure. Severely elevated calcium levels should be controlled before initial surgery, and much of the treatment for recurrent or persistent disease, including reoperative surgery, is directed at control of hypercalcemia. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Novel germline SDHD deletion associated with an unusual sympathetic head and neck paraganglioma
Authors: Cadiñanos Juan , Llorente José L. , de la Rosa Jorge , Villameytide José A. , Illán Rafael , Durán Noelia S. , Murias Eduardo , Cabanillas Rubén 
Published: March 2010

AbstractBackground.Paragangliomas (PGLs) are rare tumors arising either from sympathetic or parasympathetic‐associated chromaffin tissue. PGLs can occur either sporadically or as part of a hereditary syndrome. Sympathetic head and neck PGLs are extremely rare tumors and only a few cases have been reported to date.Methods.We report the pedigree of a patient with a head and neck PGL arising from the right sympathetic trunk. SDHD mutation analysis was performed using standard sequencing, multiplex ligation‐dependent probe amplification, chromosome 11‐specific comparative genome hybridization, and long‐range/short‐range polymerase chain reaction (PCR) approaches.Results.A previously unreported chromosome 11q deletion encompassing 5 annotated genes (SDHD, DLAT, PIH1D2, C11Orf57, and TIMM8B) was detected in the proband.Conclusion.PGL families considered “mutation‐negative” may be attributable to large gene deletions not detectable by standard sequencing methods. Therefore, deletion analysis should be offered to families or individuals at risk for hereditary PGLs. © 2010 Wiley Periodicals, Inc. Head Neck, 2011