bluuebunny/crossref_metadata_2025_split huggingface dataset:
Visual evoked potential in generalized joint hypermobility: A case–control study
Authors: Jahromi Leila Sadat Mohamadi , Sayyadi Amin , Askarian Aida , Dabbaghmanesh Alireza , Roshanzamir Sharareh 
Published: April 2024

AbstractIntroductionGeneralized joint hypermobility (GJH) can be the result of several hereditary connective tissue disorders, especially Ehlers–Danlos syndrome. Cerebrovascular manifestations are among the most common complications in this disorder, and understanding their extent can help better diagnosis and prevention of hazardous events. We investigated visual evoked potential (VEP) changes in patients with GJH and compared them with healthy individuals.MethodsOur case–control study included 90 patients who fulfilled the Beighton score (B score) for joint hypermobility and other 90 healthy participants. All of them went under VEP study, and the amplitude and latency of the evoked potential (P100) were compared to each other.ResultsThe Case group had significantly higher B score (7.18 ± 0.967 vs. 1.18 ± 0.712), P100 latency (110.23 ± 6.64 ms vs. 100.18 ± 4.273 ms), and amplitude (6.54 ± 1.26 mv vs. 6.50 ± 1.29 mv) compared with the Control group, but the difference was only significant regarding B score, and P100 latency (p‐value <.0001). Moreover, both latency and amplitude of P100 had significantly positive correlations with the B score in the Case group (p‐value <.0001), but such correlations were not found in the Control group (p‐value = .059).ConclusionOur study could reveal VEP changes, especially significant P100 latency in GJH patients without previous neurologic or musculoskeletal disorders. Whether these changes are due to GJH itself or are predictive of inevitable neurologic disease or visual pathway involvement, particularly Multiple Sclerosis needs further investigation with longer follow‐up periods.
Study on exosomes for identifying bipolar disorder in early stage: A cross‐sectional and validation study protocol
Authors: Wu Yanqing , Li Yuchao , An Xuguang , Li Jiangong , Yang Chenghao , Wang Yi 
Published: April 2024

AbstractBackgroundThe difficulty is remained to accurately distinguish bipolar disorder (BD) from major depressive disorder (MDD) in early stage, with a delayed diagnosis for 5–10 years. BD patients are often treated with antidepressants systematically due to being diagnosed with MDD, affecting the disease course and clinical outcomes. The current study aims to explore the role of plasma exosomes as biomarker to distinguish BD from MDD in early stage.MethodsTwo stages are included. The first stage is a cross‐sectional study, comparing the concentrations of plasma exosome microRNA and related proteins among BD group, MDD group, and healthy controls (HC) group (n = 40 respectively), to identify target biomarkers preliminarily. The “Latent Class Analysis” and “Receiver Operating Characteristic” analysis will be performed to determine the optimal concentration range for each biomarker. The second stage is to validate target markers in subjects, coming from an ongoing study focusing on patients with a first depressive episode. All target biomarkers will be test in plasma samples reserved at the initial stage to detect whether the diagnosis indicated by biomarker level is consistent with the diagnosis by DSM‐5. Furthermore, the correlation between specific biomarkers and the manic episode, suicidal ideation, and adverse reactions will also be observed.DiscussionExosome‐derived microRNA and related proteins have potential in serving as a good medium for exploring mental disorders because it can pass through the blood‐brain barrier bidirectionally and convey a large amount of information stably. Improving the early diagnosis of BD would help implement appropriate intervention strategy as early as possible and significantly reduce the burden of disease.
Effects of green light supplementation with red and blue combinations of LED light spectrums on the growth and transcriptional response of Haematococcus pluvialis
Authors: Karagülle G. , Telli M. 
Published: April 2024

AbstractLight management strategy is crucial for improving microalgal production in terms of higher biomass and economically valuable bioactive molecules. However, green light has received less attention in developing light managements for algae and higher plant due to its low absorption rate by chlorophyll. In this study, the effects of green light supplementation, in the combination with red and blue light were investigated in Haematococcus pluvialis. 10% and 20% of green light supplementations were applied in 3:2 ratios of red and blue LED light combinations as an expense of red‐light. Growth rates, chlorophyll concentration, and dry weight were measured to assess the growth kinetics of H. pluvialis along with the relative transcript accumulations of four mRNAs: Rubisco, PTOX2, PsaB, and PsbS. Growth rates, chlorophyll concentrations and dry weight were found significantly higher in presence of 10% green light supplementation compared to red and blue light combinations. The relative transcript accumulations of Rubisco and PsbS genes showed significant upregulation at the end of the experiments (with the fold change of 42.91 ± 12.08 and 98.57 ± 27.38, respectively, relative to the beginning of the experiments) compared to combinations of red and blue light (fold change of 19.09 ± 3.0 and 47.77 ± 14.21, respectively, relative to beginning of the experiments). PsaB and PTOX2 transcripts did not show significant accumulation differences between treatments. It seems that green light has a dose dependent additive effect on the growth rate of H. pluvialis. The upregulation of Rubisco and PsbS may indicate green light dependent carbon assimilation and light‐harvesting response in H. pluvialis.
Direct visualization and measurement of the plantar aponeurosis behavior in foot arch deformation via the windlass mechanism
Authors: Matsumoto Yuka , Ogihara Naomichi 
Published: April 2024

AbstractThe plantar aponeurosis (PA) is an elastic longitudinal band that contributes to the generation of a propulsive force in the push‐off phase during walking and running through the windlass mechanism. However, the dynamic behavior of the PA remains unclear owing to the lack of direct measurement of the strain it generates. Therefore, this study aimed to visualize and quantify the PA behavior during two distinct foot postures: (i) neutral posture and (ii) windlass posture with midtarsal joint plantarflexion and metatarsophalangeal joint dorsiflexion, using computed tomography scans. Six healthy adult males participated in the experiment, and three‐dimensional reconstruction of the PA was conducted to calculate its path length, width, thickness, and cross‐sectional area. This study successfully visualized and quantified the morphological changes in the PA induced by the windlass mechanism, providing a precise reference for biomechanical modeling. This study also highlighted the interindividual variability in the PA morphology and stretching patterns. Although the windlass posture was not identical to that observed in the push‐off phase during walking, the observed PA behavior provides valuable insights into its mechanics and potential implications for foot disorders.
Interactions of Phenylalanine Derivatives with Human Tyrosinase: Lessons from Experimental and Theoretical tudies
Authors: Faure Clarisse , Min Ng Yi , Belle Catherine , Soler‐Lopez Montserrat , Khettabi Lyna , Saïdi Mélissa , Berthet Nathalie , Maresca Marc , Philouze Christian , Rachidi Walid , Réglier Marius , du Moulinet d'Hardemare Amaury , Jamet Hélène 
Published: April 2024

AbstractThe pigmentation of the skin, modulated by different actors in melanogenesis, is mainly due to the melanins (protective pigments). In humans, these pigments’ precursors are synthetized by an enzyme known as tyrosinase (TyH). The regulation of the enzyme activity by specific modulators (inhibitors or activators) can offer a means to fight hypo‐ and hyper‐pigmentations responsible for medical, psychological and societal handicaps. Herein, we report the investigation of phenylalanine derivatives as TyH modulators. Interacting with the binuclear copper active site of the enzyme, phenylalanine derivatives combine effects induced by combination with known resorcinol inhibitors and natural substrate/intermediate (amino acid part). Computational studies including docking, molecular dynamics and free energy calculations combined with biological activity assays on isolated TyH and in human melanoma MNT‐1 cells, and X‐ray crystallography analyses with the TyH analogue Tyrp1, provide conclusive evidence of the interactions of phenylalanine derivatives with human tyrosinase. In particular, our findings indicate that an analogue of L–DOPA, namely (S)‐3‐amino‐tyrosine, stands out as an amino phenol derivative with inhibitory properties against TyH.
Metastatic pituitary neuroendocrine neoplasms: A case report of a malignant prolactinoma
Authors: Manique Inês , Amaral Sara , Rego Teresa , Coelho Andreia , Ponte Andreia , Brito Margarida , Palha Ana , Cortez Luísa , Forte Dalila , Sagarribay Amets , Cerqueira Luís , Pontinha Carlos , Mafra Manuela , Silva‐Nunes José 
Published: April 2024

Key Clinical MessageWe report a rare clinical case of a malignant prolactinoma in which the exponential increase of prolactin levels with minimal tumor growth and no response to treatment led to diagnosis of abdominal, thoracic, and vertebral metastases.
Bilateral intracochlear hemorrhage: A rare onset of chronic myelogenous leukemia
Authors: Flahat Babak , Bonnard Åsa , Arebro Julia 
Published: April 2024

Key Clinical MessageAcute onset of vertigo and hearing loss is rare in leukemic disorders. MRI can diagnose intracochlear hemorrhage as the underlying cause. The hearing can improve but if severe hearing loss preserves, cochlear implantation can be considered.
Medication‐related osteonecrosis of the jaw: Case series and literature review
Authors: Kimathi Denis , Butt Fawzia , Guthua Symon , Waweru Wairimu 
Published: April 2024

Key Clinical MessageMedication‐related osteonecrosis of the Jaw (MRONJ) is a rare complication of the jaws following the administration of antiresorptive or antiangiogenic drugs. This condition poses a major challenge to its management. Its prevention and management need a multidisciplinary collaboration. We described three patients with MRONJ including their presentation, investigations, management protocols, and outcomes. A brief appraisal of the literature on MRONJ was also done.AbstractMedication‐related osteonecrosis of the jaw (MRONJ) is a rare complication of the jaws following the administration of antiresorptive or antiangiogenic drugs. This condition poses a major challenge to its management. We present an appraisal of the literature and three cases of MRONJ. An appraisal of 3 patients who presented to the oral and maxillofacial clinic with MRONJ was done. Relevant physical examination findings, radiological images, clinical photographs and follow‐up was documented. One patient had multiple myeloma while the other two had metastatic cancer. All had received zoledronic acid before developing MRONJ. One patient was surgically treated and successfully recovered while two were managed conservatively. Patients taking antiresorptive medications are at risk of developing MRONJ. Prevention and management of the condition calls for a multidisciplinary collaboration. Patients taking antiresorptive medications need good education on the risks associated with the medications and how to recognize early signs and symptoms.
Bilateral dens evaginatus and associated cystic pathology: An unusual case report
Authors: Lee Haeseong , Le Bach , Sedghizadeh Parish 
Published: April 2024

Key Clinical MessageDens evaginatus (DE) presents a clinical conundrum due to its fragility and propensity to cause pulpal and periapical complications. Clinicians should heed the presence of DE during clinical examinations and avoid unnecessary intervention.
Arrest of labor secondary to large uterine fibroid
Authors: Ruiz Jesus 
Published: April 2024

Key Clinical MessageLarge uterine fibroids during pregnancy are associated with increased maternal and fetal complications. Large uterine fibroids should be kept in mind as a cause for arrest of labor and the need for cesarean delivery.AbstractUterine fibroids >5 cm in diameter are more likely to grow during pregnancy and cause obstetrical complications. We report a case of a large 9 cm subserosal uterine fibroid as the cause for the arrest of labor and the need for cesarean delivery.
Aromaticity in Semi‐Condensed Figure‐Eight Molecules
Authors: Artigas Albert , Carissan Yannick , Hagebaum‐Reignier Denis , Bock Harald , Durola Fabien , Coquerel Yoann 
Published: April 2024

AbstractElectron delocalization and aromaticity was comparatively evaluated in recently synthesized figure‐eight molecules made of two condensed U‐shaped polycyclic aromatic hydrocarbon moieties connected either by two single bonds or by two para‐phenylene groups. The selected examples include molecules that incorporate eight‐membered and sixteen‐membered rings, as well as a doubly [5]helicene‐bridged (1,4)cyclophane. We probe whether some electron delocalization could occur through the stereogenic single bonds in these molecules: Is aromaticity purely (semi‐)local, or possibly also global in these molecules? It was concluded that the situation can go from a purely (semi‐)local character when the dihedral angle at the connecting single bonds is large, such as in biphenyl, to a predominantly (semi‐)local character with a minor global contribution when the dihedral angle is small, such as in the para‐phenylene connectors of the [5] helicene‐bridged cyclophane.
A Bis‐Porphyrin Cavitand Breathing‐In to Constrict Bucky Balls
Authors: Kumari Anupam , Kumar Mondal Pradip , Verma Preetika , Mahato Paritosh , S Sujesh , Mandal Koushik , Polentarutti Maurizio , Lakshmanna Yapamanu Adithya , Sankar Jeyaraman 
Published: April 2024

AbstractBis‐porphyrin cages have long been exploited to bind fullerenes selectively for various applications. The major consideration for an effective binding here had been the cavity size. Herein, we structurally demonstrate that a bis‐Ni−porphyrin cavitand having even a smaller cavity can host a larger fullerene by a breathing and ruffling mechanism. It has also been shown that both the electronic and steric influence at the meso‐ positions of the porphyrin in fact dictate the binding character. The smaller cavity of 2NiD exhibits preferential binding for C70 over C60; however, surprisingly, the larger cavities in 2HD and 2NiTD display stronger affinities for C60 over the larger fullerene. We show here that the structural elasticity infused both by the metalloporphyrins and the connecting bridges play a major role in directing the binding. These conclusions have adequately been supported by structural and spectroscopic investigations. Additionally, the suitability of one of the conjugates for photoinduced charge‐separation has been investigated using ultrafast transient absorption measurements. 2NiD⊃C60 has a charge separation timescale of ~0.8 ps, while charge recombination occurs at a longer timescale of ~920 ps.
Alared: Solvatochromic and Fluorogenic Red Amino Acid for Ratiometric Live‐Cell Imaging of Bioactive Peptides
Authors: Mirloup Antoine , Berthomé Yann , Riché Stéphanie , Wagner Patrick , Hanser Fabien , Laurent Arthur , Iturrioz Xavier , Llorens‐Cortes Catherine , Karpenko Julie , Bonnet Dominique 
Published: April 2024

AbstractTo fill the need for environmentally sensitive fluorescent unnatural amino acids able to operate in the red region of the spectrum, we have designed and synthesized Alared, a red solvatochromic and fluorogenic amino acid derived from the Nile Red chromophore. The new unnatural amino acid can be easily integrated into bioactive peptides using classical solid‐phase peptide synthesis. The fluorescence quantum yield and the emission maximum of Alared‐labeled peptides vary in a broad range depending on the peptide's environment, making Alared a powerful reporter of biomolecular interactions. Due to its red‐shifted absorption and emission spectra, Alared‐labeled peptides could be followed in living cells with minimal interference from cellular autofluorescence. Using ratiometric fluorescence microscopy, we were able to track the fate of the Alared‐labeled peptide agonists of the apelin G protein‐coupled receptor upon receptor activation and internalization. Due to its color‐shifting environmentally sensitive emission, Alared allowed for distinguishing the fractions of peptides that are specifically bound to the receptor or unspecifically bound to different cellular membranes.
Prediction of Bond Dissociation Energy for Organic Molecules Based on a Machine‐Learning Approach
Authors: Liu Yidi , Li Yao , Yang Qi , Yang Jin‐Dong , Zhang Long , Luo Sanzhong 
Published: April 2024

Comprehensive SummaryBond dissociation energy (BDE), which refers to the enthalpy change for the homolysis of a specific covalent bond, is one of the basic thermodynamic properties of molecules. It is very important for understanding chemical reactivities, chemical properties and chemical transformations. Here, a machine learning‐based comprehensive BDE prediction model was established based on the iBonD experimental BDE dataset and the calculated BDE dataset by St. John et al. Differential Structural and PhysicOChemical (D‐SPOC) descriptors that reflected changes in molecules’ structural and physicochemical features in the process of bond homolysis were designed as input features. The model trained with LightGBM algorithm gave a low mean absolute error (MAE) of 1.03 kcal/mol on the test set. The D‐SPOC model could apply to accurate BDE prediction of phenol O—H bonds, uncommon N‐SCF3 and O‐SCF3 reagents, and β‐C—H bonds in enamine intermediates. A fast online prediction platform was constructed based on the D‐SPOC model, which could be found at http://isyn.luoszgroup.com/bde_prediction.
A Selenium Atom Involved Covalent Organic Framework for Window Ledge Photocatalytic Oxidation of Sulfides
Authors: Yang Fan , Li Xia , Qu Hong‐Yan , Kan Jing‐Lan , Guo Yuan , Dong Yu‐Bin 
Published: April 2024

Comprehensive SummaryCovalent organic frameworks (COFs) driven photocatalytic organic transformations especially photooxidation reactions have become a fertile topic and attracted numerous research attentions. Boosting the charge generation and transport process is the key factor for achieving high catalytic efficiencies. As one of the most effective strategies, the introduction of “heavy atoms” into the long‐range ordered conjugated backbones can effectively facilitate the intersystem crossing (ISC) process and hence improve the generation of active oxygens, which is beneficial for the oxidation. In this work, we designed and synthesized a benzoselenadiazole based covalent organic framework (COF) material, BSe‐COF with heavy atom of selenium (Se), and a benzothiadiazole based BT‐COF with isomorphic backbone for comparison. Compared to BT‐COF, BSe‐COF exhibits broader absorption range, stronger photocurrent response and enhanced intersystem crossing (ISC) with higher singlet oxygen (1O2) generation efficiency. When applied in photocatalytic organic transformation, BSe‐COF presents remarkably higher photocatalytic activity in the oxidation of sulfides than BT‐COF under the irradiation of blue LED lamp. Furthermore, BSe‐COF can be used as efficient photocatalyst for the window ledge reaction with high yields (over 84%) of various sulfoxides from a wide range of thioether substrates scope.
Catalytic Asymmetric Synthesis of Inherently Chiral Saddle‐Shaped Dibenzo[b,f][1,5]diazocines†
Authors: Zhou Jinmiao , Tang Mengyao , Yang Xiaoyu 
Published: April 2024

Comprehensive SummaryDibenzo[b,f][1,5]diazocines are a class of eight‐membered heterocycles, which exhibit unique rigid saddle‐shaped structure and possess inherent chirality. In this study, we report a convenient and straightforward method for the catalytic enantioselective synthesis of these unique chiral molecules through chiral phosphoric acid‐catalyzed dimerization of 2‐acylbenzoisocyanates. Notably, the addition of corresponding 2‐acylaniline as the co‐catalyst significantly improved the efficiency of these reactions, and a simple phase separation operation resulted in products with excellent enantiopurity. Experimental studies were performed to elucidate the mechanism behind these reactions, leading to the proposal of a plausible reaction mechanism based on the study findings.
Exploring Raloxifene‐Based Metallodrugs: A Versatile Vector Combined with Platinum(II), Palladium(II) and Nickel(II) Dichlorides and Carborates against Triple‐Negative Breast Cancer
Authors: Kazimir Aleksandr , Götze Tom , Lönnecke Peter , Murganić Blagoje , Mijatović Sanja , Maksimović‐Ivanić Danijela , Hey‐Hawkins Evamarie 
Published: April 2024

AbstractTriple‐negative breast cancer (TNBC) poses challenges in therapy due to the absence of target expression such as estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). Frequently, the treatment of TNBC involves the combination of several therapeutics. However, an enhanced therapeutic effect can be also achieved within a single molecule. The efficacy of raloxifene can be improved by designing a raloxifene‐based hybrid drug bearing a 2,2’‐bipyridine moiety (2). Integration of platinum(II), palladium(II), and nickel(II) complexes into this structure dramatically changed the cytotoxicity. The platinum(II) dichloride complex 3 did not demonstrate any activity, while palladium(II) and nickel(II) dichloride complexes 4 and 5 exhibited various cytotoxic behavior towards different types of hormone‐receptor positive (HR+) cancer and TNBC cell lines. The replacement of the two chlorido ligands in 3–5 with a dicarbollide (carborate) ion [C2B9H11]2− resulted in reduced activity of compounds 6, 7, and 8. However, the palladacarborane complex 7 demonstrated higher selectivity towards TNBC. Furthermore, the mechanism of action was shifted from cytotoxic to explicitly cytostatic with detectable proliferation arrest and accelerated aging, characterized by senescence‐associated phenotype of TNBC cells. This study provides valuable insights into the development of hybrid therapeutics against TNBC.