bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genetic Diversity of Local Rainfed Rice (<i>Oryza Sativa</i> L.)
Authors: Siddique MA , Rashid ESMH , Khalequzzaman M , Islam MZ , Ahmed MS , Baktiar MHK 
Published: December 2011

Genetic diversity of 58 traditional local rice genotypes was studied under rainfed condition through Mahalanobis D2 statistic for grain yield and yield contributing characters. The genotypes were grouped into nine clusters. The inter-cluster distances were higher than intra-cluster distances indicating wider genetic diversity among the genotypes of different clusters. The intra-cluster distances were lower in all the cases reflecting homogeneity of the genotypes within the clusters. The cluster III contained the highest number of genotypes (13) and the cluster I contained the lowest (2). The highest intra-cluster distance was noticed for the cluster I and the lowest for cluster VII. The highest inter-cluster distance was observed between cluster I and IV followed by cluster IX and IV, cluster I and VI and the lowest between cluster V and III. Regarding inter-cluster distance, the genotypes of cluster IV showed high genetic distance from all other clusters. The genotypes from cluster IV could be hybridized with the genotypes of other characters of other clusters for producing transgressive segregants. Based on positive value of vector 1 and vector 2, flag leaf length, plant height, panicle length and grain breath had maximum contribution towards genetic divergence. The highest cluster means for yield, 1000 grain weight and grain breath were obtained from cluster VIII. The highest plant height, flag leaf width and panicle length were found in cluster II while the lowest mean value for yield, days to flowering and maturity, unfilled grain and the highest mean value for grain length breadth ratio were found in cluster II. Therefore, more emphasis should be given on cluster IV and cluster VIII, cluster I and cluster IV for selecting genotypes as parents for crossing with the genotypes of cluster II which would be used to produce new recombinants with desired traits. Key words: Genetic diversity; D2 analysis; cluster analysis; rice (Oryza sativa L)DOI: http://dx.doi.org/10.3329/bjpbg.v23i2.9324 Bangladesh J. Pl. Breed. Genet., 23(2): 41-46, 2010
Reproducibility of Macular Thickness Measurement Among Five OCT Instruments: Effects of Image Resolution, Image Registration, and Eye Tracking
Authors: Chin Eric K. , Sedeek Ragui W. , Li Yueju , Beckett Laurel , Redenbo Ellen , Chandra Karishma , Park Susanna S. 
Published: December 2011

BACKGROUND AND OBJECTIVE:
To study the effect of image resolution, eye tracking, and image registration on central macular thickness reproducibility (rCMT) among spectral-domain and time-domain optical coherence tomography (SD-OCT and TD-OCT) instruments.

PATIENTS AND METHODS:
Seventy-six eyes were imaged (44 normal, 32 maculopathy) either twice using four SD-OCT and one TD-OCT devices or three times using Spectralis SD-OCT (with and without eye tracking) (Heidelberg Engineering, Inc., Heidelberg, Germany). Cirrus images (Carl Zeiss Meditec, Dublin, CA) were further analyzed with three-point image registration.

RESULTS:

All instruments had superior rCMT in normal versus pathologic eyes (
P
< .001). No difference in rCMT was noted among instruments in normal eyes (
P
= .92), but TD-OCT was superior to SD-OCT (
P
= .017) in pathologic eyes. Cirrus image registration improved rCMT for normal eyes (
P
= .04), with borderline improvement in pathologic eyes (
P
= .06). Spectralis eye tracking improved rCMT in normal (
P
= .01) and pathologic (
P
= .004) eyes.

CONCLUSION:
Higher image resolution with SD-OCT may not improve rCMT, but image registration and eye tracking options may improve rCMT.
Testicular touch preparation cytology in the evaluation of male infertility
Authors: Yildiz-Aktas Isil Z. , Monaco Sara E. , Khalbuss Walid E. , Parwani Anil V. , Jaffe Thomas M. , Pantanowitz Liron 
Published: December 2011

Background:Male infertility is traditionally evaluated by tissue core biopsies of the testes. Touch preparations (TP) of these biopsies have been infrequently used. The aim of this study is to report our experience with using testicular biopsy TP for the evaluation of male infertility.Materials and Methods:A retrospective search was performed for cases of testes biopsies with concurrent TP. These cases were evaluated for clinical information, specimen adequacy, and cytological–histological correlation.Results:A total of 39 cases were identified from men with a mean age of 34 years (range 23 to 50 years). TP slides were satisfactory for evaluation in 31 (89%) cases, and less than optimal in four due to low cellularity, obscuring blood or air drying artifact. Cytopathology showed concordance with the biopsy in almost all cases. In one discordant case where the biopsies showed no active spermatogenesis, a rare sperm were identified on the TP.Conclusions:TP of the testis is a helpful adjunct to biopsy because of its ability to clearly evaluate all stages of spermatogenesis. These data demonstrate that TP cytopathology of the testes in our experience has an excellent correlation with both normal testicular biopsies and those showing pathological spermatogenesis, and in rare cases may provide added benefit in evaluating the presence of spermatogenesis for male infertility. Albeit uncommon, cytopathologists may be required to identify and evaluate spermatogenic elements in cytology specimens being submitted from men with infertility.
Sex Disparities After Coronary Artery Bypass Grafting and Hospital Quality
Authors: Wagner Catherine M. , Ibrahim Andrew M. 
Published: June 2024

ImportanceConcern has been raised about persistent sex disparities after coronary artery bypass grafting, with female patients having higher mortality. However, whether these disparities persist across hospitals of different qualities is unknown.ObjectiveTo evaluate sex disparities in 30-day mortality after coronary artery bypass grafting across high- and low-quality hospitals.Design, Setting, and ParticipantsThis cross-sectional, retrospective cohort study evaluated Medicare beneficiaries undergoing coronary artery bypass grafting between October 1, 2015, and March 31, 2020. Data analysis was performed from July 1, 2023, to December 1, 2023.ExposuresThe primary exposures were hospital quality and sex. For hospital quality, hospitals were placed into rank order by their overall risk-adjusted mortality rate and divided into quintiles.Main Outcome and MeasuresRisk-adjusted 30-day mortality using a logistic regression model accounting for patient factors, including sex, age, comorbidities, elective vs unplanned admission, number of bypass grafts, use of arterial graft, and year of surgery.ResultsA total of 444 855 beneficiaries (mean [SD] age, 71.5 [7.5] years; 120 333 [27.1%] female and 324 522 [72.9%] male) were studied. Compared with male beneficiaries, female beneficiaries were more likely to have an unplanned admission (66 425 [55.2%] vs 157 895 [48.7%], P &amp;lt; .001) and receive care at low-quality (vs high-quality) hospitals (odds ratio, 1.26; 95% CI, 1.23-1.29; P &amp;lt; .001). Overall, risk-adjusted female mortality was 4.24% (95% CI, 4.20%-4.27%), and male mortality was 2.75% (95% CI, 2.75%-2.77%), with an absolute difference of 1.48 (95% CI, 1.45-1.51) percentage points (P &amp;lt; .001). At the highest-quality hospitals, male mortality was 1.57% (95% CI, 1.56%-1.59%), and female mortality was 2.58% (95% CI, 2.54%-2.62%), with an absolute difference of 1.01 (95% CI, 0.97-1.04) percentage points (P &amp;lt; .001). At the lowest-quality hospitals, male mortality was 4.94% (95% CI, 4.88%-5.01%), and female mortality was 7.02% (95% CI, 6.90%-7.13%), with an absolute difference of 2.07 (95% CI, 1.95-2.19) percentage points (P &amp;lt; .001). Female beneficiaries receiving care at low-quality hospitals had a higher mortality than male beneficiaries receiving care at the high-quality hospitals (7.02% vs 1.57%, P &amp;lt; .001).Conclusions and RelevanceIn this cohort study of Medicare beneficiaries undergoing coronary artery bypass grafting, female beneficiaries were more likely to receive care at low-quality hospitals, where the sex disparity in mortality was double that of high-quality hospitals. Quality improvement targeting low-quality hospitals as well as equitable referral of female beneficiaries to higher-quality hospitals may narrow the sex disparity after coronary artery bypass grafting.
Clinicopathological features and cancer transcriptomic profiling of poorly cohesive gastric carcinoma subtypes
Authors: Yen Hung‐Hsuan , Chen Pin‐Yu , Huang Ruby Yun‐Ju , Jeng Jung‐Ming , Lai I‐Rue 
Published: June 2024

AbstractGastric poorly cohesive carcinoma (PCC) manifests with a diffuse pattern and diverse tumor cell morphologies, often indicating a more unfavorable prognosis. Recent consensus has reclassified PCC based on the proportion of signet‐ring cells (SRCs) in tumors for research purposes. The two most distinct subtypes, poorly cohesive carcinoma not otherwise specified (PCC‐NOS) and signet‐ring cell carcinoma (SRCC), are characterized by less than 10% and more than 90% SRCs, respectively. However, research comparing the clinicopathological and transcriptomic differences between these subtypes remains limited. In this study, we conducted a comparative analysis of clinicopathological features in 55 advanced‐stage PCCs, consisting of 43 PCC‐NOS and 12 SRCC cases. Subsequently, 12 PCC‐NOS and 5 SRCC cases were randomly selected for initial cancer‐related gene expression profiling and pathway enrichment analysis using the GeoMx digital spatial profiler, followed by validation in a separate validation group comprising 16 PCC‐NOS and 6 SRCC cases. These transcriptomic findings were then correlated with tumor morphology and clinicopathological data. PCC‐NOS cases exhibited larger tumor size, a higher prevalence of pathological N3 disease, and a worse 1‐year progression‐free survival rate compared to SRCC cases. Clustering of PCC‐NOS and SRCC was successfully achieved using the GeoMx Cancer Transcriptome Atlas. Among all studied genes, only MMP7 showed differential expression, with its overexpression significantly associated with the PCC‐NOS subtype, increased perineural invasion, and earlier disease progression. Pathway analysis revealed significantly enriched pathways in PCC‐NOS related to vesicle‐mediated transport, adaptive immune systems, oncogenic signaling, and extracellular matrix organization, while SRCC displayed significant enrichment in pathways associated with respiratory electron transport and the cell cycle. In conclusion, this study compares and correlates clinicopathological features and transcriptomic data between PCC‐NOS and SRCC at advanced stages, employing the latest consensus classification and a novel platform for analysis.
Endovascular thrombectomy versus medical management on outcomes with infarct volumes more than 70 mL
Authors: Han Nannan , Zhang Xiaobo , Zhang Yu , Liu Yu , Ma Haojun , Ge Hanming , Wang Yanfei , Li Shilin , Yan Xudong , Li Tengfei , Wu Yulun , Ma Juan , Shi Wenzhen , Zhang Gejuan , Tian Ye , Chang Mingze 
Published: June 2024

AbstractObjectiveEndovascular thrombectomy (EVT) in patients with large infarct volume remains controversial. The aim of this study is to compare clinical outcomes between EVT and medical management in acute large vessel occlusion with infarct volumes larger than 70 mL on diffusion‐weighted magnetic resonance imaging (DWI).MethodsA prospective observational cohort study was conducted, including patients with anterior cerebral circulation occlusion due to ischemic stroke with infarct volumes larger than 70 mL within 24 h of onset between July 2018 and June 2023. Eligible patients were divided into two groups: the EVT group and the medical management (non‐EVT) group. The main outcomes were functional independence and mortality at 90 days. To assess clinical endpoints, we selected variables including age, NIHSS score, infarct volume, and occlusion location for 1:1 propensity score (PS) matching and PS adjustment using inverse probability of treatment weighting (IPTW).ResultsAmong the 131 identified patients (mean [SD] age, 69.9 [13.7] years; 58 female), the median infarct volume was 123.6 mL. Of these patients, 75 (57.3%) underwent EVT. After PS adjustment, EVT was not associated with functional independence (10.9% vs. 10.9%; p = 1.000) or mortality (43.5% vs. 47.8%; p = 0.675). Additionally, after PS adjustment using IPTW, EVT was also not associated with a functional independence (15.8% vs. 13.7%; p = 0.767) or mortality (46.8% vs. 44.0%; p = 0.762).ConclusionThis study provides real‐world evidence regarding infarct volumes larger than 70 mL, indicating that EVT does not provide benefits compared to medical management alone when considering age, NIHSS score, infarct volume, and occlusion location.
Exhaustive clinical examination of etiology and initial response to first‐line treatment in 577 children with infantile epileptic spasm syndrome children: A 5‐year retrospective observational study
Authors: Wan Lin , Ge Wenrong , Liu Guoyin , He Wen , Liang Yan , Dun Shuo , Yan Huimin , Chen Jian , Zhu Gang , Gao Jing , Shi Xiuyu , Wang Jing , Hu Linyan , Zhang Bo , Zou Liping , Yang Guang 
Published: June 2024

AbstractObjectiveEmploying whole‐exome sequencing (WES) technology to investigate the etiology of infantile epileptic spasm syndrome (IESS), and determining whether different etiologies exhibit phenotypic variations, while elucidating the potential associated factors, might improve short‐term responses to first‐line treatment.MethodsWe retrospectively evaluated patients with IESS admitted for treatment between January 2018 and June 2023. Clinical phenotypic differences among etiological classifications and clinical manifestations were analyzed. Variable selection using the best subset method was performed, followed by logistic regression analysis to identify the factors influencing treatment response.ResultsA total of 577 patients were included; 412 completed trio‐WES. Magnetic resonance imaging abnormalities were detected in 387 patients (67.1%). Patients with etiology as structural abnormalities were likelier to have non‐spasms at the initial seizure onset. A total of 532 patients completed the first‐line treatment; 273 patients received it for the first time at our hospital (initial response rates: 30.1% and 42.1%, respectively). The response group had a lower proportion of early‐onset seizures (≤3 months) than the no‐response group (11.3% vs. 23.7%, p < 0.01 and 11.3% vs. 21.5%, p = 0.03, respectively). Logistic regression analysis indicated that earlier initiation of first‐line treatment was associated with a higher likelihood of an initial response. However, the etiological classification did not have a significant impact on the initial response.InterpretationIESS patients with structural abnormalities are more likely to present with non‐spasm seizures at initial onset. Early initiation of first‐line treatment is crucial; however, initial responses may be less favorable when seizures occur in early infancy.
Assembled Embedded 3D Hydrogel System for Asynchronous Drug Delivery to Inhibit Postoperative Recurrence of Malignant Glioma and Promote Neurological Recovery
Authors: Hu Yang , Zhou Liming , Wang Zhenning , Ye Zhiming , Liu Huiling , Lu Yi , Qi Zhihui , Yang Kunhua , Zeng Jianhao , Li Huimin , Tang Ruizhe , Ren Jiaoyan , Guo Rui , Yao Maojin 
Published: June 2024

AbstractSurgical resection of glioblastoma multiforme (GBM) often results in tumor recurrence and mild neurologic deficits. Here, a 3D asynchronous drug delivery system is innovatively developed to address the dual challenges of GBM recurrence and postoperative neurological deficit. Based on transcriptome analysis of tumor cells and tumor microenvironment (TME) cells between primary and recurrent mouse GBM tissues, a novel dual‐targeting approach is developed to combine mTOR pathway inhibition with microglia/macrophage repolarization. Then, in situ injectable methacrylated gelatin (GelMA) is constructed to perfectly fit into the tumor resection cavity and achieve direct delivery of dual‐targeted drugs, exhibiting outstanding postoperative GBM inhibitory effects in vivo. At the same time, neurotrophic factor‐saturated 3D‐printed GelMA patches are used to construct a 3D asynchronous drug delivery system, allowing gradual penetration of the neurotrophic factors into the underlying hydrogel to promote axonal sprouting after GBM suppression. Notably, this 3D asynchronous drug delivery system promotes neurological recovery without weakening the efficacy of inhibiting tumor recurrence. Therefore, this study not only proposes a new dual‐targeted GBM treatment strategy but also pioneers the construction of a 3D asynchronous drug delivery system for the comprehensive treatment of GBM. This study is expected to improve the poor prognosis of patients with GBM.
Roadmap toward Controlled Ion Beam‐Induced Defects in 2D Materials
Authors: Telkhozhayeva Madina , Girshevitz Olga 
Published: June 2024

AbstractUnderstanding the nature, density, and distribution of structural defects is crucial for tailoring the properties of atomically thin two‐dimensional (2D) materials, which is paramount for advances in nanotechnology. Ion irradiation emerges as a promising technique for defect engineering of single‐atom‐thick materials, due to its high controllability, repeatability, and accuracy. The objective is to provide a comprehensive review elucidating the impact of various irradiation parameters, such as ion mass, energy, fluence, and incident angle, on defect formation in 2D materials. However, the presence of the substrate can significantly influence defect yield and the mechanism of formation due to backscattered ions and sputtered substrate atoms. Hence, a thorough comparison of ion beam‐induced defects in both freestanding (suspended) and supported (on a substrate) 2D materials, with a focus on substrate effects is conducted. Moreover, a detailed analysis of characterization techniques suitable for each scenario will be provided. This work not only contributes to advancing the current understanding of defect formation and evolution in 2D materials during ion beam irradiation but also offers insights into selecting specific parameters for this process to create desired defects in these materials. Consequently, it has the potential to facilitate the design of nanoscale devices with tailored functionality.
Prussian Blue‐Derived Nanocomposite Synergized with Calcium Overload for Three‐Mode ROS Outbreak Generation to Enhance Oncotherapy
Authors: Xu Wenting , Zhou Hongmei , Hu Bangli , Liang Xinqiang , Tang Yanping , Ning Shufang , Ding He , Yang Piaoping , Wang Chen 
Published: June 2024

AbstractCalcium overload can lead to tumor cell death. However, because of the powerful calcium channel excretory system within tumor cells, simplistic calcium overloads do not allow for an effective antitumor therapy. Hence, the nanoparticles are created with polyethylene glycol (PEG) donor‐modified calcium phosphate (CaP)‐coated, manganese‐doped hollow mesopores Prussian blue (MMPB) encapsulating glucose oxidase (GOx), called GOx@MMPB@CaP‐PEG (GMCP). GMCP with a three‐mode enhancement of intratumor reactive oxygen species (ROS) levels is designed to increase the efficiency of the intracellular calcium overload in tumor cells to enhance its anticancer efficacy. The released exogenous Ca2+ and the production of cytotoxic ROS resulting from the perfect circulation of the three‐mode ROS outbreak generation that Fenton/Fenton‐like reaction and consumption of glutathione from Fe2+/Fe3+and Mn2+/Mn3+ circle, and amelioration of hypoxia from MMPB‐guided and GOx‐mediated starvation therapy. Photothermal efficacy‐induced heat generation owing to MMPB accelerates the above reactions. Furthermore, abundant ROS contribute to damage to mitochondria, and the calcium channels of efflux Ca2+ are inhibited, resulting in a calcium overload. Calcium overload further increases ROS levels and promotes apoptosis of tumor cells to achieve excellent therapy.
Triggerable Patches for Medical Applications
Authors: Sirolli Sofia , Guarnera Daniele , Ricotti Leonardo , Cafarelli Andrea 
Published: June 2024

AbstractMedical patches have garnered increasing attention in recent decades for several diagnostic and therapeutic applications. Advancements in material science, manufacturing technologies, and bioengineering have significantly widened their functionalities, rendering them highly versatile platforms for wearable and implantable applications. Of particular interest are triggerable patches designed for drug delivery and tissue regeneration purposes, whose action can be controlled by an external signal. Stimuli‐responsive patches are particularly appealing as they may enable a high level of temporal and spatial control over the therapy, allowing high therapeutic precision and the possibility to adjust the treatment according to specific clinical and personal needs. This review aims to provide a comprehensive overview of the existing extensive literature on triggerable patches, emphasizing their potential for diverse applications and highlighting the strengths and weaknesses of different triggering stimuli. Additionally, the current open challenges related to the design and use of efficient triggerable patches, such as tuning their mechanical and adhesive properties, ensuring an acceptable trade‐off between smartness and biocompatibility, endowing them with portability and autonomy, accurately controlling their responsiveness to the triggering stimulus and maximizing their therapeutic efficacy, are reviewed.
Invoking Interfacial Engineering Boosts Structural Stability Empowering Exceptional Cyclability of Ni‐Rich Cathode
Authors: Chu Youqi , Mu Yongbiao , Gu Huicun , Hu Yan , Wei Xianbin , Zou Lingfeng , Yu Can , Xu Xiaoqian , Kang Shaowei , Li Kang , Han Meisheng , Zhang Qing , Zeng Lin 
Published: June 2024

AbstractThe cycling stability of LiNi0.8Co0.1Mn0.1O2 under high voltages is hindered by the occurrence of hybrid anion‐ and cation‐redox processes, leading to oxygen escape and uncontrolled phase collapse. In this study, an interfacial engineering strategy involving a straightforward mechanical ball milling and low‐temperature calcination, employing a Se‐doped and FeSe2&Fe2O3‐modified approach is proposed to design a stable Ni‐rich cathode. Se2− are selectively adsorbed within oxygen vacancies to form O─TM─Se bond, effectively stabilizing lattice oxygen, and preventing structural distortion. Simultaneously, the Se‐NCM811//FeSe2//Fe2O3 self‐assembled electric field is activated, improving interfacial charge transfer and coupling. Furthermore, FeSe2 accelerates Li+ diffusion and reacts with oxygen to form Fe2O3 and SeO2. The Fe2O3 coating mitigates hydrofluoric acid erosion and acts as an electrostatic shield layer, limiting the outward migration of oxygen anions. Impressively, the modified materials exhibit significantly improved electrochemical performance, with a capacity retention of 79.7% after 500 cycles at 1C under 4.5 V. Furthermore, it provides an extraordinary capacity retention of 94.6% in 3–4.25 V after 550 cycles in pouch‐type full battery. This dual‐modification approach demonstrates its feasibility and opens new perspective for the development of stable lithium‐ion batteries operating at high voltages.