bluuebunny/crossref_metadata_2025_split huggingface dataset:
Oncogenic KRAS‐induced interleukin‐8 overexpression promotes cell growth and migration and contributes to aggressive phenotypes of non‐small cell lung cancer
Authors: Sunaga Noriaki , Imai Hisao , Shimizu Kimihiro , Shames David S. , Kakegawa Seiichi , Girard Luc , Sato Mitsuo , Kaira Kyoichi , Ishizuka Tamotsu , Gazdar Adi F. , Minna John D. , Mori Masatomo 
Published: May 2011

AbstractThe CXC chemokine interleukin‐8 (IL‐8) is an angiogenic growth factor that is overexpressed in various cancers, including non‐small cell lung cancer (NSCLC). Previously, IL‐8 was shown as a transcriptional target of RAS signaling, raising the possibility of its role in oncogenic KRAS‐driven NSCLC. Using microarray analysis, we identified IL‐8 as the most downregulated gene by shRNA‐mediated KRAS knockdown in NCI‐H1792 NSCLC cells where IL‐8 is overexpressed. NSCLC cell lines harboring KRAS or EGFR mutations overexpressed IL‐8, while IL‐8 levels were more prominent in KRAS mutants compared to EGFR mutants. IL‐8 expression was downregulated by shRNA‐mediated KRAS knockdown in KRAS mutants or by treatment with EGFR tyrosine kinase inhibitors and EGFR siRNAs in EGFR mutants. In our analysis of the relationship of IL‐8 expression with clinical parameters and mutation status of KRAS or EGFR in 89 NSCLC surgical specimens, IL‐8 expression was shown to be significantly higher in NSCLCs of males, smokers, and elderly patients and those with pleural involvement and KRAS mutated adenocarcinomas. In KRAS mutant cells, the MEK inhibitor markedly decreased IL‐8 expression, while the p38 inhibitor increased IL‐8 expression. Attenuation of IL‐8 function by siRNAs or a neutralizing antibody inhibited cell proliferation and migration of KRAS mutant/IL‐8 overexpressing NSCLC cells. These results indicate that activating mutations of KRAS or EGFR upregulate IL‐8 expression in NSCLC; IL‐8 is highly expressed in NSCLCs from males, smokers, elderly patients, NSCLCs with pleural involvement, and KRAS‐mutated adenocarcinomas; and IL‐8 plays a role in cell growth and migration in oncogenic KRAS‐driven NSCLC.
Increased risk of hepatocellular carcinoma in patients with diabetes mellitus: A systematic review and meta‐analysis of cohort studies
Authors: Wang Chenggang , Wang Xiaolin , Gong Gaoquan , Ben Qiwen , Qiu Weili , Chen Yi , Li Guoping , Wang Liangwen 
Published: May 2011

AbstractIn recent years, increasing evidence has suggested a strong association between diabetes mellitus (DM) and hepatocellular carcinoma (HCC). To provide a quantitative assessment of this association, we performed a systematic review and meta‐analysis of cohort studies. We collected studies through a literature search of Medline from January 1, 1966 and EMBASE from January 1, 1974, through July 31, 2010. Summary relative risks (SRRs) with their corresponding 95% confidence intervals (CIs) were calculated using a random‐effects model. A total of 25 cohort studies that met our inclusion and exclusion criteria were included in our analysis. Among these, 18 studies showed that DM was associated with an increased incidence of HCC (SRRs = 2.01, 95% CI: 1.61–2.51), compared with individuals without DM. There was a statistically significant heterogeneity among these studies (Q = 136.68, p < 0.001, I2 = 87.6%). Analyses subgrouped by controlling confounders revealed that the increased incidence of HCC was independent of geographic location, alcohol consumption, history of cirrhosis, or infections with hepatitis B (HBV) or hepatitis C virus (HCV). In addition, DM was also positively associated with HCC mortality (SRR = 1.56; 95% CI: 1.30–1.87), with no significant evidence of heterogeneity among studies (Q = 1.16, p = 0.56, I2 =0%). There were no significant publication bias (p = 0.79 for Egger's regression asymmetry test). These findings strongly support a positive association between DM and increased risk of HCC in both males and females.
Aryl hydrocarbon receptor nuclear translocator is associated with tumor growth and progression of hepatocellular carcinoma
Authors: Liang Ying , Li Wei‐Wei , Yang Bi‐Wei , Tao Zhong‐Hua , Sun Hui‐Chuan , Wang Lu , Xia Jing‐Lin , Qin Lun‐Xiu , Tang Zhao‐You , Fan Jia , Wu Wei‐Zhong 
Published: May 2011

AbstractbHLH/PAS proteins play important roles in tumor progression. Lost or reduced expression of single‐minded homolog 2 (SIM) as well as aryl hydrocarbon receptor repressor (AHRR) has been observed in cancerous human tissues. Here, we investigated the role of aryl hydrocarbon receptor nuclear translocator (ARNT), another bHLH/PAS protein, in hepatocellular carcinoma (HCC). Using tissue microarray and immunohistochemistry, we found that intratumoral ARNT was inversely correlated with time to recurrence and overall survival of HCC patients after resection. Knockdown of ARNT in HepG2, HCCLM3 and HCCLM6 cells significantly shortened cell doubling time, increased S‐phase cell populations and accelerated in vivo HCCLM6 growth and metastasis. After ARNT expression was rescued, prolonged cell doubling time and decreased S‐phase cell populations were observed in HepG2, HCCLM3 and HCCLM6 cells. And, HCCLM6 growth and metastasis in vivo were remarkably inhibited. Screening by quantitative reverse‐transcription PCR and PCR arrays revealed that cyclin E1, CDK2, Fos and Jun were negatively regulated by ARNT, whereas CDKN1C, CNKN2A, CDKN2B, MAPK11 and MAPK14 were positively regulated in HCC. According to the results of immunoprecipitation assay, both ARNT/ARNT and ARNT/AHRR complexes were clearly formed in HCCLM6 xenograft with increased ARNT expression. In summary, ARNT is an important regulator of HCC growth and metastasis and could be a promising prognostic candidate in HCC patients.
Supramolecular Assemblies of Thioflavin T with Cucurbiturils: Prospects of Cooperative and Competitive Metal Ion Binding
Authors: Bhasikuttan Achikanath C. , Dutta Choudhury Sharmistha , Pal Haridas , Mohanty Jyotirmayee 
Published: May 2011

AbstractOf late, molecular‐recognition‐guided supramolecular assembly and its relevance in the development of highly stable and active photofunctional materials have drawn much attention. In this review, the non‐covalent interaction of a biologically important dye, thioflavin T (ThT), with cucurbituril macrocycles, especially, cucurbit[7]uril (CB7) and cucurbit[8]uril (CB8), and the response of the resulting molecular assemblies towards the metal ions have been discussed. The interaction of ThT with CB7 leads to significant enhancement in the fluorescence yield, lifetime and modifications in the spectral features of ThT. These changes are assigned to the formation of 1 : 1 and 2 : 1 (CB7⋅ThT) complexes. However, with CB8 a distinct evolution of a hitherto unexplored strong excimer emission band having maximum at 570 nm is observed. The strong ion–dipole interactions provided by the carbonyl portals of the CB8 adequately support the stabilization of two π‐stacked ThTs, both in 1 : 2 (CB8⋅ThT) and 2 : 2 stoichiometric ratio. In case of the 1 : 1 (CB7⋅ThT) system, the metal ion addition leads to the usual competitive binding interaction and decreases the fluorescence intensity. However, the addition of the same metal ion to the 2 : 1 (CB7⋅ThT) complex results in a novel cooperative metal ion binding to the complex, leading to the formation of a highly fluorescent supramolecular capsule. Further addition of amantadine hydrochloride induces rupture of the capsular complex, projecting potential application in targeted drug delivery. The 2 : 2 (CB8⋅ThT) complex responds to the metal ion presence in a competitive way, which allows demonstration of a fluorescence on–off mechanism.
Cucurbiturils as Versatile Receptors for Redox Active Substrates
Authors: Kaifer Angel E. , Li Wei , Yi Song 
Published: May 2011

AbstractResearch on the chemistry of cucurbit[n]uril (CBn) hosts has picked up and maintained an impressive pace in the last decade, primarily due to the isolation of hosts with relatively larger cavity sizes, such as CB7 and CB8. This review article summarizes our involvement in this research effort, with particular emphasis on the binding of redox active guests by the CB7 and CB8 hosts. The binding of 4,4′‐bipyridinium (viologen) derivatives was the starting point of our CB research. While methylviologen is encapsulated by CB7, forming a highly symmetric inclusion complex, more hydrophobic viologens are bound by inclusion of one of the terminal N‐substituents inside the host cavity. Cationic ferrocene derivatives reach extremely high binding affinities with CB7. Binding by CB8 offers additional possibilities, since this host may accommodate two aromatic units inside its cavity, which can be utilized to exert redox control on the assembly of suitably dendronized guests. From a purely electrochemical standpoint, CB7‐included viologens maintain their voltammetric reversibility, but CB7‐included ferrocene residues experience a pronounced attenuation of their electron transfer kinetics. We have also applied these binding and electrochemical properties to the design and preparation of switchable, CB‐based pseudorotaxanes.
Grafting of dermatan sulfate on polyethylene terephtalate to enhance biointegration
Authors: Dhahri Manel , Abed Aicha , Lajimi Ramzi Hadj , Mansour Mohamed Ben , Gueguen Virginie , Abdesselem Saber Ben , Chaubet Frederic , Letourneur Didier , Meddahi‐Pellé Anne , Maaroufi Raoui M. 
Published: May 2011

AbstractThe aim of the present study was to achieve the immobilization of dermatan sulfate (DS) on polyethylene terephthalate (PET) surfaces and to evaluate its biocompatibility. DS obtained from the skin of Scyliorhinus canicula shark was immobilized via carbodiimide on knitted PET fabrics, modified with carboxyl groups. PET‐DS characterization was performed by SEM, ATR‐FTIR and contact angle measurements. Biocompatibility was evaluated by investigating plasma protein adsorption and endothelial cell proliferation, as well as by subcutaneous implantations in rats. The results indicated that DS immobilization on PET was achieved at ∼8 μg/cm2. ATR‐FTIR evidenced the presence of sulfate groups on the PET surface. In turn, contact angle measurements indicated an increase in the surface wettability. DS immobilization increased albumin adsorption on the PET surface, whereas it decreased that of fibrinogen. In vitro cell culture revealed that endothelial cell proliferation was also enhanced on PET‐DS. Histological results after 15 days of subcutaneous implantation showed a better integration of PET‐DS samples in comparison to those of nonmodified PET. In summary, DS was successfully grafted onto the surface of PET, providing it new physicochemical characteristics and biological properties for PET, thus enhancing its biointegration. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
CaSiO3 microstructure modulating the in vitro and in vivo bioactivity of poly(lactide‐co‐glycolide) microspheres
Authors: Wu Chengtie , Zhang Yufeng , Fan Wei , Ke Xuebin , Hu Xuye , Zhou Yinghong , Xiao Yin 
Published: May 2011

AbstractPoly(lactide‐co‐glycolide) (PLGA) microspheres have been used for regenerative medicine due to their ability for drug delivery and generally good biocompatibility, but they lack adequate bioactivity for bone repair application. CaSiO3 (CS) has been proposed as a new class of material suitable for bone tissue repair due to its excellent bioactivity. In this study, we set out to incorporate CS into PLGA microspheres to investigate how the phase structure (amorphous and crystal) of CS influences the in vitro and in vivo bioactivity of the composite microspheres, with a view to the application for bone regeneration. X‐ray diffraction (XRD), N2 adsorption‐desorption analysis, and scanning electron microscopy (SEM) were used to analyze the phase structure, surface area/pore volume, and microstructure of amorphous CS (aCS) and crystal CS (cCS), as well as their composite microspheres. The in vitro bioactivity of aCS and cCS–PLGA microspheres was evaluated by investigating their apatite‐mineralization ability in simulated body fluids (SBF) and the viability of human bone mesenchymal stem cells (BMSCs). The in vivo bioactivity was investigated by measuring their de novo bone‐formation ability. The results showed that the incorporation of both aCS and cCS enhanced the in vitro and in vivo bioactivity of PLGA microspheres. cCS/PLGA microspheres improved better in vitro BMSC viability and de novo bone‐formation ability in vivo, compared to aCS/PLGA microspheres. Our study indicates that controlling the phase structure of CS is a promising method to modulate the bioactivity of polymer microsphere system for potential bone tissue regeneration. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
Synthesis of GTMAC modified chitin–PAA gel and evaluation of its biological properties
Authors: Uppanan Paweena , Channasanon Somruethai , Veeranondh Sukitaya , Tanodekaew Siriporn 
Published: May 2011

AbstractThe dressing prepared from GTMAC modified chitin–PAA was introduced with the aim of facilitating wound healing, particularly those effectively absorbing exudates, maintaining a moist wound environment and controlling bacterial proliferation. Chitin was chemically modified with acrylic acid to encourage a moist wound healing environment. The highly water‐absorbable resulting product (chitin–PAA) was further reacted with glycidyltrimethylammonium chloride (GTMAC) to impart antibacterial activities. The final product, chitin–PAA–GTMAC was characterized by the techniques of Fourier Transform Infrared (FTIR), solid state 15N NMR, and elemental analysis. Their cytotoxicity and antibacterial activities against S. epidermidis and E. coli were evaluated which found increasing effects in those properties with increasing degree substitution of GTMAC. All materials also showed good blood‐clotting ability. The collagen gel contraction assay was used to analyze the behavior of fibroblasts after contact with the gels. The extent of the gel contraction as well as the examination of the secreted interleukin‐8 (IL‐8) and transforming growth factor‐β1 (TGF‐β1) were investigated. The results showed that chitin‐PAA modified with GTMAC could stimulate the production of IL‐8, but TGF‐β1. Fibroblasts presented normal spreading and formation of cellular processes in the collagen gels with all of the modifications. Furthermore, all modified gels except for the highest GTMAC content gel [chitin–PAA–GTMAC (1:20)] were found a greater extent in gel contraction than the unmodified chitin–PAA. It suggested the promoting effect of GTMAC on cell proliferation if the GTMAC content in the gel was not too high, that is, the mole ratio of glucosamine to GTMAC of the gel should not greater than 1:10. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
Molecular plasma deposited peptides on anodized nanotubular titanium: An osteoblast density study
Authors: Balasundaram Ganesan , Shimpi Tushar M. , Sanow William R. , Storey Daniel M. , Kitchell Barbara S. , Webster Thomas J. 
Published: May 2011

AbstractA large amount of work is currently being conducted to design, fabricate, and characterize materials coated or immobilized with bioactive molecules for tissue engineering applications. Here, a novel method, molecular plasma deposition (MPD), is introduced with can efficiently coat materials with numerous bioactive peptides. Specifically, here, RGDS (arginine‐glycine‐aspartic acid‐serine), KRSR (lysine‐arginine‐serine‐arginine), and IKVAV (isoleucine‐lysine‐valine‐alanine‐valine) were coated on anodized nanotubular titanium using MPD. The anodized nanotubular titanium surfaces were characterized using scanning electron microscopy (SEM), atomic force microscopy (AFM), and water contact angle measurements. Peptide coatings were examined by X‐ray photoelectron spectroscopy (XPS) and an amine reactive fluorescence molecule, 3‐(4 carboxybenzoyl)quinoline 2‐carboxaldehyde (CBQCA). Electrospray ionization (ESI) was used to confirm peptide integrity. Osteoblast (bone‐forming cell) density was determined on the materials of interest. Results confirmed peptide coatings and showed that the MPD RGDS and KRSR coatings on anodized nanotubular titanium increased osteoblast density compared with uncoated substrates and those coated with IKVAV and a control peptide (RGES) after 4 h and 7 days. SEM confirmed differences in the morphology of the attached cells. These results, to the best of our knowledge, are the first reports using MPD to efficiently create peptide coatings to increase osteoblast density on metals commonly used in orthopedics. Since MPD represents a quick, inexpensive, and versatile technique to coat implants with peptides, it should be further studied for numerous implant applications. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
Light microscopic description of the effects of laser phototherapy on bone defects grafted with mineral trioxide aggregate, bone morphogenetic proteins, and guided bone regeneration in a rodent model
Authors: Pinheiro Antonio L. B. , Soares Luiz G. P. , Aciole Gilberth T. S. , Correia Neandder A. , Barbosa Artur F. S. , Ramalho Luciana M. P. , dos Santos Jean N. 
Published: May 2011

AbstractWe carried out a histological analysis on bone defects grafted with mineral trioxide aggregate (MTA) treated or not with laser, bone morphogenetic protein (BMP), and guided bone regeneration (GBR). Benefits of the use of MTA, laser, BMPs, and GBR on bone repair are well known, but there is no report on their association with laser light. Ninety rats were divided into 10 groups each subdivided into 3. Defects on G II and I were filled with the blood clot. G II was further irradiated with LED. G III and IV were filled with MTA; G IV was further irradiated with laser. G V and VI, the defects filled with MTA and covered with a membrane (GBR). G VI was further irradiated with laser. G VII and VIII, BMPs were added to the MTA and group VIII further irradiated with laser. G IX and X, the MTA + BMP graft was covered with a membrane (GBR). G X was further irradiated with laser. Laser light (λ = 850 nm, 150 mW, 4 J/cm2) was applied over the defect at 48‐h intervals and repeated for 15 days. Specimens were processed, cut and stained with H&E and Sirius red and underwent histological analysis. Subjects on group X were irradiated. The results showed different tissue response on all groups during the experimental time. Major changes were seen on irradiated subjects and included marked deposition of new bone in advanced maturation. It is concluded that near infrared laser phototherapy improved the results of the use of the MTA on bone defects. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
The cytotoxicity assessment of the novel latex urinary catheter with prolonged antimicrobial activity
Authors: Kowalczuk Dorota , Ginalska Grazyna , Przekora Agata 
Published: May 2011

AbstractThe purpose of this study was to evaluate the in vitro biocompatibility of the novel Sparfoxacin (SPA)‐treated latex catheter with previously performed prolonged antimicrobial activity. Rectangular‐shaped test samples of silicone latex catheter were fabricated according to patented procedure permitting the immobilization of SPA on heparin (HP)‐coated catheter by means of mixed, covalent and non‐covalent bonds. Samples subjected to cytotoxicity assay were divided into four groups: (1) the untreated catheter, (2) HP‐coated catheter, (3) HP‐coated catheter with SPA immobilized in low SPA concentration solution (SPA‐L treated sample), and (4) high SPA concentration solution (SPA‐H treated sample). Then the samples were placed directly into green monkey kidney (GMK) cell monolayer for 24 h. After the incubation period, cytotoxicity was determined by 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyl‐2H‐tetrazolium bromide (MTT) assay. The degree of cytotoxicity of each sample was evaluated according to the reference value represented by the control cells cultured without catheter sample. Statistical significance was determined by repeated t‐test (P < 0.05). The cytotoxic effect of treated and untreated catheters was also estimated by microscopic observations of GMK cells morphological changes. SPA‐treated catheters demonstrated high survival rates in MTT assay (>93%) on the contrary to the untreated catheters (6.13%) and HP‐coated catheters (80.90%). Moreover, microscopic observation of GMK cells exposed to SPA‐treated samples revealed no morphological changes and no cell growth reduction. We suggest that SPA‐treated latex catheters are biofilm formation resistant (as we revealed in our previous work), considerably less toxic than untreated ones, and can be undoubtedly used in urological practice. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2011.
Skeletal muscle fat content is inversely associated with bone strength in young girls
Authors: Farr Joshua N , Funk Janet L , Chen Zhao , Lisse Jeffrey R , Blew Robert M , Lee Vinson R , Laudermilk Monica , Lohman Timothy G , Going Scott B 
Published: May 2011

Abstract
Childhood obesity is an established risk factor for metabolic disease. The influence of obesity on bone development, however, remains controversial and may depend on the pattern of regional fat deposition. Therefore, we examined the associations of regional fat compartments of the calf and thigh with weight-bearing bone parameters in girls. Data from 444 girls aged 9 to 12 years from the Jump-In: Building Better Bones study were analyzed. Peripheral quantitative computed tomography (pQCT) was used to assess bone parameters at metaphyseal and diaphyseal sites of the femur and tibia along with subcutaneous adipose tissue (SAT, mm2) and muscle density (mg/cm3), an index of skeletal muscle fat content. As expected, SAT was positively correlated with total-body fat mass (r = 0.87–0.89, p &lt; .001), and muscle density was inversely correlated with total-body fat mass (r = −0.24 to −0.28, p &lt; .001). Multiple linear regression analyses with SAT, muscle density, muscle cross-sectional area, bone length, maturity, and ethnicity as independent variables showed significant associations between muscle density and indices of bone strength at metaphyseal (β = 0.13–0.19, p &lt; .001) and diaphyseal (β = 0.06–0.09, p &lt; .01) regions of the femur and tibia. Associations between SAT and indices of bone strength were nonsignificant at all skeletal sites (β = 0.03–0.05, p &gt; .05), except the distal tibia (β = 0.09, p = .03). In conclusion, skeletal muscle fat content of the calf and thigh is inversely associated with weight-bearing bone strength in young girls. © 2011 American Society for Bone and Mineral Research