bluuebunny/crossref_metadata_2025_split huggingface dataset:
External factors driving surface temperature changes above geothermal systems: answers from deep learning
Authors: Giannoulis Michail , Pailot-Bonnétat Sophie , Barra Vincent , Harris Andrew 
Published: June 2024

Introduction: The surface expression of enhanced geothermal heat fluxes above an active hydrothermal system causes a surface thermal anomaly (ΔT). The thermal anomaly is expressed by the difference between the temperature within the heated zone (Th) and the temperature of non-heated surfaces (T0). Given that the resulting thermal anomaly at the surface is of extremely low magnitude (1°C–5°C at Vulcano, Italy), it is extremely sensitive to overprinting by external factors, namely, meteorological influences on surface temperature variation, such as solar heating, wind and rain.Methods: To test the sensitivity of the surface to external drivers, we installed two surface temperature measurement stations within the Vulcano’s Fossa crater, one inside the thermal anomaly and one outside (separation = 50 m), with a weather station co-located with the T0 station. Time series of Th and T0 were collected for 2020, when the Vulcano Fossa hydrothermal system was at a low and stable level of activity so that external drivers would have been the main influences on Th and T0, and hence also ΔT. To test for divergence from normality in terms of diurnal and seasonal variations in Th and T0, and the role of external factors in causing abnormality, we used the deep learning engine DITAN: a domain-agnostic framework to detect and interpret anomalies in time-series data.Results: During the year, DITAN found 16 cases of two types of meteorological events: intense low-pressure systems and high-intensity rainstorms (cloudbursts). Passage of 13 abnormal low-pressure systems were detected (10 between February and May, and three in December), with three abnormal rainstorm events (all in December); all three being coincident with the abnormal low pressure events. We find just two abnormalities in the time series for of Th and T0, both of which coincide with passage of abnormal low-pressure systems, and neither of which coincide with abnormal rain events. We conclude that diurnal and annual heating and cooling cycles, subject to normal meteorological inputs and at a surface above a geothermal-heated source, are immune to anomalous behaviour to the external (meteorological) variations.
Integrated assessment of flood susceptibility and exposure rate in the lower Niger Basin, Onitsha, Southeastern Nigeria
Authors: Chinedu Ani D. , Ezebube Nkiruka M. , Uchegbu Smart , Ozorme Vivian A. 
Published: June 2024

BackgroundVarious methods have been utilized to investigate and mitigate flood occurrences, yet there is a paucity of literature on factors, such as soil compositions, that contribute to persistent flooding in river basins like the Lower Niger catchment, specifically at Onitsha. Furthermore, the study seeks to furnish essential geospatial data concerning flood vulnerability, flood risks, and exposure rates in the Lower Niger Catchment area, situated in Onitsha, southeastern Nigeria.Materials and methodsSoil samples were collected from 10 specific locations identified through GPS and ground-truthing techniques. Additionally, satellite imagery from the Landsat Enhanced Thematic Mapper (ETM +) was utilized, with supervised classification employed to extract feature classes. Analysis operations were conducted using IDRISI software, resulting in the creation of digital elevation models (DEMs), susceptibility maps, and flood-risk zones.ResultsAnalysis revealed that the predominant soil composition in the study area comprises sandy (84.8%), silt (8.1%), and clayey (7.1%) soils. Utilizing these soil characteristics alongside relevant aerial data, exposure rates were determined at various scales to delineate the most flood-vulnerable zones in the basin. It was found that certain areas, accommodating a population exceeding 79,426 across 2,926.2 ha, were particularly susceptible to flooding. Notably, major markets such as Bridgehead, Textile, and Biafra were identified as highly susceptible, with varying degrees of risk. The prevalence of sandy soil, which facilitates increased rainwater infiltration but is also prone to rapid saturation and runoff, likely contributes to the heightened susceptibility to flooding in these areas.ConclusionGeospatial analysis employing remote sensing data indicates the high susceptibility and exposure to flooding in the lower Niger River Basin around Onitsha. Urgent mitigation efforts are imperative, necessitating the establishment of zoned areas equipped with effective drainage systems to safeguard vulnerable populations.
Synergy of carboxymethyl cellulose stabilized nanoscale zero-valent iron and Penicillium oxalicum SL2 to remediate Cr(VI) contaminated site soil
Authors: Pan Siyi , Tong Jianhao , Luo Yating , Pang Jingli , Zhang Haonan , Wang Jing , Shi Jiyan 
Published: June 2024

Nano zero-valent iron (nZVI) acting as a high-cost disposable material in soil Cr(VI) remediation faces significant challenges due to its easily oxidizable nature and biological toxicity. In addressing this issue, the present study undertook the synthesis of a series of modified nZVI and combined the selected material with Cr(VI)-resistant filamentous fungus Penicillium oxalicum SL2 for real-site chromium pollution remediation. Adsorption experiments demonstrated that the inclusion of carboxymethyl cellulose (CMC) significantly enhanced the adsorption capacity of nZVI for Cr(VI) by 19.3% (from 73.25 to 87.4 mg/L), surpassing both biochar (37.42 mg/L) and bentonite modified nZVI (48.03 mg/L). Characterization results validated the successful synthesis of the nano composite material. Besides, oxidative stress analysis explained the unique detoxification effects of CMC on SL2, acting as a free radical scavenger and isolating layer. In real-sites soil remediation experiments, a low dosage (0.4% w/w) of nZVI/CMC@SL2 (CMC modified nZVI combined with SL2) exhibited an impressive reduction of over 99.5% in TCLP-Cr(VI) and completely transformed 18% of unstable Cr to stable forms. Notably, nZVI/CMC demonstrated its capability to facilitate SL2 colonization in highly contaminated soil and modulate the microbial community structure, enriching chromium-removing microorganisms. In summary, the synergistic system of nZVI/CMC@SL2 merges as a cost-effective and efficient approach for Cr(VI) reduction, providing meaningful insights for its application in the remediating contaminated site soils.
Exploring the impacts of land use and land cover change on ecosystem services in Dongting Lake, China: a spatial and temporal analysis
Authors: Chen Zhefu , Liu Yang , Chen Duanlv , Peng Baofa 
Published: June 2024

Background and objectives: Land use and land cover change (LULCC) directly affects the human living environment and economic society by influencing ecosystem services. This study explores the complex relationship between LULCC and ecosystem services in the Dongting Lake region of China.Methods: The analysis incorporates the transfer matrix analysis of LULCC, ecosystem service value (ESV) accounting, Mann–Kendall mutation test, gray correlation, and path analysis. This study explored the spatial and temporal characteristics of LULCC and ecosystem services and revealed the comprehensive impacts of LULCC on ecosystem services from the perspective of factor interactions.Findings: The findings reveal that open water (OW) area and farmable land are the dominant land use types transferring in and out of the study area, with significant interconversion observed among open water area, wetland (WL), arable land (AL), and forest land (FL). Water conservation, waste disposal, climate regulation, and biodiversity conservation emerge as the most crucial ecosystem services in the study area. The study identifies consistent patterns of abrupt changes in LULCC and ESV across six analyzed time periods. Forest land exhibits the most substantial direct influence on climate regulation, gas exchange, soil formation and protection, conservation of biodiversity, raw materials, and recreation and leisure. Additionally, forest land, wetland, arable land, and open water area contribute significantly to the total ESV, with forest land being the dominant factor influencing the spatial differentiation of ecosystem services in the Dongting Lake region.Conclusion: The research reveals the consistency of ecosystem services and LULCC trends in the Dongting Lake area. LULCC is a complex process influenced by multiple factors, in which the conversion between ecological and non-ecological land use has a significant impact, and the complex coupling and coordination among LULCC drivers together influence ecosystem services. Furthermore, LULCC alters the structure and scale of ecosystems, resulting in alterations in the ESV.
Lessons learned: avoiding bias via multi-state analysis of patients’ trajectories in real-time
Authors: Lucke Elisabeth , Hazard Derek , Grodd Marlon , Weber Susanne , Wolkewitz Martin 
Published: June 2024

ObjectivesMany studies have attempted to determine the disease severity and patterns of COVID-19. However, at the beginning of the pandemic, the complex patients’ trajectories were only descriptively reported, and many analyses were worryingly prone to time-dependent-, selection-, and competing risk biases. Multi-state models avoid these biases by jointly analysing multiple clinical outcomes while taking into account their time dependency, including current cases, and modelling competing events. This paper uses a publicly available data set from the first wave in Israel as an example to demonstrate the benefits of analysing hospital data via multi-state methodology.MethodsWe compared the outcome of the data analysis using multi-state models with the outcome obtained when various forms of bias are ignored. Furthermore, we used Cox regression to model the transitions among the states in a multi-state model. This allowed for the comparison of the covariates’ influence on transition rates between the two states. Lastly, we calculated expected lengths of stay and state probabilities based on the multi-state model and visualised it using stacked probability plots.ResultsCompared to standard methods, multi-state models avoid many biases in the analysis of real-time disease developments. The utility of multi-state models is further highlighted through the use of stacked probability plots, which visualise the results. In addition, by stratification of disease patterns by subgroups and visualisation of the distribution of possible outcomes, these models bring the data into an interpretable form.ConclusionTo accurately guide the provision of medical resources, this paper recommends the real-time collection of hospital data and its analysis using multi-state models, as this method eliminates many potential biases. By applying multi-state models to real-time data, the gained knowledge allows rapid detection of altered disease courses when new variants arise, which is essential when informing medical and political decision-makers as well as the general population.
Regulatory role of Mycobacterium tuberculosis MtrA on dormancy/resuscitation revealed by a novel target gene-mining strategy
Authors: Fu Xiang , Wan Xiaoyu , Memon Aadil Ahmed , Fan Xiao-Yong , Sun Qiuhong , Chen Haifeng , Yao Yufeng , Deng Zixin , Ma Jian , Ma Wei 
Published: June 2024

IntroductionThe unique dormancy of Mycobacterium tuberculosis plays a significant role in the major clinical treatment challenge of tuberculosis, such as its long treatment cycle, antibiotic resistance, immune escape, and high latent infection rate.MethodsTo determine the function of MtrA, the only essential response regulator, one strategy was developed to establish its regulatory network according to high-quality genome-wide binding sites.Results and discussionThe complex modulation mechanisms were implied by the strong bias distribution of MtrA binding sites in the noncoding regions, and 32.7% of the binding sites were located inside the target genes. The functions of 288 potential MtrA target genes predicted according to 294 confirmed binding sites were highly diverse, and DNA replication and damage repair, lipid metabolism, cell wall component biosynthesis, cell wall assembly, and cell division were the predominant pathways. Among the 53 pathways shared between dormancy/resuscitation and persistence, which accounted for 81.5% and 93.0% of the total number of pathways, respectively, MtrA regulatory genes were identified not only in 73.6% of their mutual pathways, but also in 75.4% of the pathways related to dormancy/resuscitation and persistence respectively. These results suggested the pivotal roles of MtrA in regulating dormancy/resuscitation and the apparent relationship between dormancy/resuscitation and persistence. Furthermore, the finding that 32.6% of the MtrA regulons were essential in vivo and/or in vitro for M. tuberculosis provided new insight into its indispensability. The findings mentioned above indicated that MtrA is a novel promising therapeutic target for tuberculosis treatment since the crucial function of MtrA may be a point of weakness for M. tuberculosis.
24-h continuous non-invasive multiparameter home monitoring of vitals in patients with Rett syndrome by an innovative wearable technology: evidence of an overlooked chronic fatigue status
Authors: Leoncini Silvia , Boasiako Lidia , Di Lucia Sofia , Beker Amir , Scandurra Valeria , Vignoli Aglaia , Canevini Maria Paola , Prato Giulia , Nobili Lino , Nicotera Antonio Gennaro , Di Rosa Gabriella , Chiarini Maria Beatrice Testa , Cutrera Renato , Grosso Salvatore , Lazzeri Giacomo , Tongiorgi Enrico , Morano Pasquale , Botteghi Matteo , Barducci Alessandro , De Felice Claudio 
Published: June 2024

BackgroundSleep is disturbed in Rett syndrome (RTT), a rare and progressive neurodevelopmental disorder primarily affecting female patients (prevalence 7.1/100,000 female patients) linked to pathogenic variations in the X-linked methyl-CpG-binding protein 2 (MECP2) gene. Autonomic nervous system dysfunction with a predominance of the sympathetic nervous system (SNS) over the parasympathetic nervous system (PSNS) is reported in RTT, along with exercise fatigue and increased sudden death risk. The aim of the present study was to test the feasibility of a continuous 24 h non-invasive home monitoring of the biological vitals (biovitals) by an innovative wearable sensor device in pediatric and adolescent/adult RTT patients.MethodsA total of 10 female patients (mean age 18.3 ± 9.4 years, range 4.7–35.5 years) with typical RTT and MECP2 pathogenic variations were enrolled. Clinical severity was assessed by validated scales. Heart rate (HR), respiratory rate (RR), and skin temperature (SkT) were monitored by the YouCare Wearable Medical Device (Accyourate Group SpA, L’Aquila, Italy). The average percentage of maximum HR (HRmax%) was calculated. Heart rate variability (HRV) was expressed by consolidated time-domain and frequency-domain parameters. The HR/LF (low frequency) ratio, indicating SNS activation under dynamic exercise, was calculated. Simultaneous continuous measurement of indoor air quality variables was performed and the patients’ contributions to the surrounding water vapor partial pressure [PH2O (pt)] and carbon dioxide [PCO2 (pt)] were indirectly estimated.ResultsOf the 6,559.79 h of biovital recordings, 5051.03 h (77%) were valid for data interpretation. Sleep and wake hours were 9.0 ± 1.1 h and 14.9 ± 1.1 h, respectively. HRmax % [median: 71.86% (interquartile range 61.03–82%)] and HR/LF [median: 3.75 (interquartile range 3.19–5.05)] were elevated, independent from the wake–sleep cycle. The majority of HRV time- and frequency-domain parameters were significantly higher in the pediatric patients (p ≤ 0.031). The HRV HR/LF ratio was associated with phenotype severity, disease progression, clinical sleep disorder, subclinical hypoxia, and electroencephalographic observations of multifocal epileptic activity and general background slowing.ConclusionOur findings indicate the feasibility of a continuous 24-h non-invasive home monitoring of biovital parameters in RTT. Moreover, for the first time, HRmax% and the HR/LF ratio were identified as potential objective markers of fatigue, illness severity, and disease progression.
The effect of transcranial electrical stimulation on the relief of mental fatigue
Authors: Chen Ruijuan , Huang Lengjie , Wang Rui , Fei Jieying , Wang Huiquan , Wang Jinhai 
Published: June 2024

ObjectiveThe presence of mental fatigue seriously affects daily life and working conditions. Non-invasive transcranial electrical stimulation has become an increasingly popular tool for relieving mental fatigue. We investigated whether transcranial direct current stimulation (tDCS) and transcranial alternating current stimulation (tACS) could be used to alleviate the state of mental fatigue in a population of healthy young adults and compared their effects.MethodsWe recruited 10 participants for a blank control, repeated measures study. Each participant received 15 min of anodal tDCS, α-tACS, and blank stimulation. Participants were required to fill in the scale, perform the test task and collect ECG signals in the baseline, fatigue and post-stimulus states. We then assessed participants’ subjective fatigue scale scores, test task accuracy and HRV characteristics of ECG signals separately.ResultsWe found that both anodal tDCS and α-tACS significantly (P &lt; 0.05) reduced subjective fatigue and improved accuracy on the test task compared to the blank group, and the extent of change was greater with tACS. For the HRV features extracted from ECG signals. After tACS intervention, SDNN (t = −3.241, P = 0.002), LF (t = −3.511, P = 0.001), LFn (t = −3.122, P = 0.002), LFn/HFn (−2.928, P = 0.005), TP (t = −2.706, P = 0.008), VLF (t = −3.002, P = 0.004), SD2 (t = −3.594, P = 0.001) and VLI (t = −3.564, P = 0.001) showed a significant increasing trend, and HFn (t = 3.122, P = 0.002), SD1/SD2 (t = 3.158, P = 0.002) and CCM_1 (t = 3.106, P = 0.003) showed a significant decreasing trend. After tDCS intervention, only one feature, TINN, showed a significant upward trend (P &lt; 0.05). The other features showed non-significant changes but roughly the same trend as the tACS group.ConclusionBoth tDCS and α-tACS can be effective in relieving mental fatigue, and α-tACS is more effective than tDCS. This study provides theoretical support for tDCS with α-tACS having a alleviating effect on mental fatigue and the use of ECG as a valid objective assessment tool.
Exploring the causal relationship between interleukin-6 or C reactive protein and malignant melanoma using a two-sample Mendelian randomization approach
Authors: Wang Quan Jun , Zheng Wei , Pan Sun Feng 
Published: June 2024

The goal was to explore the effect of interleukin-6 (IL6) and C reactive protein (CRP) on malignant melanoma (MM) using two-sample Mendelian randomization.MethodsData for this study were obtained from the IEU Open GWAS project website for genome-wide association study data (GWAS) on interleukin-6, C reactive protein levels and malignant melanoma. Inverse variance weighted (IVW) method was mainly used and supplemented with MR-Egger regression and weighted median. Finally, horizontal multivariate validity and heterogeneity tests were performed to assess the stability and reliability of the results.ResultsThe results of univariate two-sample MR analyses showed no significant effect of CRP on MM: inverse variance weighting method (OR=0.999, 95% CI: 0.998–1.001, P=0.343), MR-Egger regression (OR= 1.000, 95% CI: 0.998–1.001, P= 0.180), and weighted median method (OR= 0.999, 95% CI: 0.997 to 1.000, P= 0.583), and weighted model (OR= 0.999, 95% CI: 0.998 to 1.001, P= 0.328). Also,IL-6 had no significant effect on MM: inverse variance weighting method (OR= 1.001, 95% CI: 0.999 to 1.002, P=0.461), MR-Egger regression (OR= 1.000, 95% CI: 0.997 to 1.004, P= 0.910), weighted median method (OR= 1.000, 95% CI: 0.998 to 1.002, P= 0.749), and weighted mode (OR= 1.000, 95% CI: 0.998 to 1.002, P= 0.820).ConclusionThere was no causal relationship between C-reactive protein and IL-6 on the risk of malignant melanoma.
Niraparib for the treatment of metastatic ccRCC in a patient with CDK12 and RAD51C mutations: a case report
Authors: Yue Xiaolong , Yang Chenkang , Cao Dandan , Li Yue 
Published: June 2024

BackgroundNiraparib, a poly ADP-ribose polymerase inhibitors (PARPi), has been widely applied in the intervention of epithelial ovarian, fallopian tube, or primary peritoneal cancer. Nevertheless, as of the present moment, there are limited instances demonstrating favorable outcomes stemming from niraparib therapy in patients with clear cell renal cell carcinoma (ccRCC).Case presentationHere, we report a case of a 50-year-old patient with ccRCC who subsequently developed distant metastasis. The patient received monotherapy with pazopanib and combination therapy with axitinib and tislelizumab, demonstrating limited efficacy. Liquid biopsy revealed missense mutations in the CDK12 and RAD51C of the homologous recombination repair (HRR) pathway, suggesting potential sensitivity to PARPi. Following niraparib treatment, the patient’s condition improved, with no significant side effects.ConclusionIn summary, patients with ccRCC harboring HRR pathway gene mutation may potentially benefit from niraparib. This will present more options for ccRCC patients with limited response to conventional treatments.
Exploring Mo-ZnO@NF for hydrogen generation and methylene blue remediation: sunlight-driven catalysis
Authors: Padwal Yogita , Chauhan Ratna , Panchang Rajani , Fouad Hassan , Gosavi Suresh W. 
Published: June 2024

In this study, we elucidate the synthesis and characterization of molybdenum (Mo) doped zinc oxide (ZnO) nanoflowers (Mo-ZnO@NF) fabricated via a hydrothermal approach, showcasing their potential application in hydrogen generation and dye degradation. The successful synthesis of these nanoflowers is achieved through the deliberate incorporation of Mo ions into the ZnO lattice, yielding a distinctive hierarchical flower-like morphology. Comprehensive structural, morphological, and optical analyses are conducted employing a suite of analytical techniques, encompassing XRD, Raman, FESEM, and UV-Visible spectroscopy. XRD analysis confirms the retention of the hexagonal wurtzite crystal structure, accompanied by discernible peak shifts indicative of Mo ion integration. FESEM imaging further elucidates the flower-like architecture of Mo-ZnO, underscoring the intricate morphological features. Photocatalytic assessment reveals the remarkable efficacy of Mo-ZnO@NF, as evidenced by an unprecedented hydrogen evolution rate of 2024 mmol/h/g and 97% Methylene Blue (MB) dye degradation within a mere 40-minute timeframe. Furthermore, a comparative investigation between pristine ZnO and varying Mo doping concentrations (ranging from 1% to 5%) underscores the optimal doping concentration of 1% Mo in ZnO. This concentration threshold is shown to engender superior photocatalytic performance, potentially attributed to enhanced charge carrier separation and increased surface area conducive to catalytic reactions. Overall, this study not only advances our understanding of Mo-ZnO@NF nanostructures but also elucidates key insights into optimizing their photocatalytic efficacy for diverse environmental remediation applications.