bluuebunny/crossref_metadata_2025_split huggingface dataset:
Procedure to Identify and Submit cDNA Sequences to
Authors: Britton Jr. George L. , Islam Ahmad S. , Xuan Yue , Sathasivan Kanagasabapathi 
Published: April 2011

With rapid advances in DNA sequencing and large collection of such information available, it may be helpful to have a simplified procedure for analysis and submission of such sequence information. The process has been described in three steps: (a) data gathering and preparation, (b) data analysis, and (c) data submission to Genbank. The use of NCBI-tools such as VecScreen and ORF finder has been shown to be effective in detecting the vector contaminated sequences and in identifying the segments that code for protein with ‘start-’ and ‘stop’ codon, respectively. Another software called ‘A Plasmid Editor’ is useful in reconstructing the complete DNA sequence of a gene from the sequences of complementary strand. The process of sequence data submission to the GenBank about the gene of interest is described in an easy-to-follow manner, including the final step of receiving an accession number from NCBI. Key words: Jute cDNA library, Corchorus olitorius, VecScreen, ORF Finder, Vector Contamination, GenBank, BankIt, Heat shock protein, TAIR WUBLAST D.O.I. 10.3329/ptcb.v17i2.3236 Plant Tissue Cult. & Biotech. 17(2): 161-172, 2007 (December)
Novel ESTs from a Jute (Corchorus olitorius L.) cDNA Library
Authors: Wazni Mohamad W. , Islam Ahmad S. , Taliaferro J. Matthew , Anwar Nabila , Sathasivan Kanagasabapathi 
Published: April 2011

The paper describes the identification of new expressed sequence tags (ESTs) from a cDNA library of Corchorus olitorius L. var. O-4 after the initial description of the library construction reported recently. The sequence homology search in The Arabidopsis Information Source (TAIR) using the WU-BLAST tool revealed four complete and ten partial cDNA sequences. The complete cDNA sequences, based on their similarities to those of Arabidopsis proteins, encode V-ATPase subunit F, mitochondrial NADH-ubiquinone oxidoreductase, RuBisCO small subunit 1A and heat shock protein (HSP 60). Based on similar homology results the partial cDNAs encode proteins, namely, chaperonin, actin 7, RelA-spoT homology, 60s ribosomal protein L 36a, transport protein, chloroplast inner membrane import protein Tic22, formate dehydrogenase, serine hydroxy-methyltransferase, metallothionein 2B and tansmembrane kinase. These cDNA sequences are new additions to the ESTs that encode chitinase-like protein (Class I) and 60S acidic ribosomal protein reported earlier. All the available cDNA sequences including that for the chitinase-like protein have been registered with GenBank, bearing Accession Nos. EU024510 through EU024520 and EU092254, EU057193-95. The cDNA samples are available for any researchers interested in such ESTs. A Clustal-W study of the amino acid sequences of proteins encoded by cDNA isolated in the present investigation with those of cotton, citrus, Arabidopsis, tobacco and other organisms revealed that the homology is maximum between jute and cotton followed by citrus, grapevine, tobacco and Arabidopsis. Key words: Jute cDNA library, Corchorus olitorius, Chitinase, Chaperonin, RSH2, RelA/SpoT, V-ATPase, Small subunit RuBisCO, Metallothionein, Actin 7, and HSPs D.O.I. 10.3329/ptcb.v17i2.3237 Plant Tissue Cult. & Biotech. 17(2): 173-182, 2007 (December)
Study of blood lead and semen lead concentration in male infertility
Authors: Debnath BC , Ibrahim M , Fatima P 
Published: April 2011

Objective: To evaluate the association of blood and semen lead with male infertility. Design: It was a case control study carried out in the department of Biochemistry, BSMMU, from January, 2004 to December, 2004.Subjects: Of 52 male subjects, 26 were infertile with oligospermia and / or asthenospermia. Age matched 26 normospermic subjects with normal standard semen parameters (volume, count, motility and morphology) were selected as controls. None had the history of occupational exposure to lead.Results: Median values of blood lead were 19.96 µg/dl and 17.68 µg/dl (normal <25 µg/dl); semen lead were 29.56µg/dl and 28.17 µg/dl (normal 15.37 ± 3.92 µg/dl) in cases and controls respectively. Neither blood lead nor semen lead showed significant difference between cases and controls. There was significant positive correlation between blood lead and semen lead concentrations. There was no significant correlation of semen and blood lead concentrations with other semen parameters & serum hormone concentrations (FSH, LH, & Testosterone) in cases. Conclusion: Though the association of blood or semen lead with male infertility was not established in our study, yet, increased seminal lead concentration found in our study subjects may be indicative of subtle exposure of lead in our environment. So, further study with larger sample size is needed. The clinicians should consider the lead measurements when evaluating male partners of couples with unexplained infertility and for assessment of lead status, semen lead measurement can be recommended.DOI: http://dx.doi.org/10.3329/pulse.v4i1.6955Pulse Vol.4 January 2010 p.10-13
Parents en deuil
Authors: Oppenheim Daniel 
Published: April 2011

Résumé Parents en deuil – La mort d’un enfant est une des expériences de deuil les plus douloureuses, dans tous les pays. Malgré des avancées significatives ces dernières années, l’aide que ceux-ci peuvent recevoir reste encore limitée. Tous les soignants peuvent être des interlocuteurs de parents en deuil et pas seulement ceux qui se sont occupés de leur enfant. La mort d’un enfant laisse souvent des traces dans l’histoire de sa famille et dans le devenir de ses membres : les parents, un enfant né après, la fratrie, y compris plus tard quand elle devient adulte, voire sur ses enfants. Les entretiens individuels ou les groupes psychothérapeutiques aident les parents à traverser leur deuil. Ils apportent une connaissance précieuse des processus de deuil. Quatre éléments ont marqué l’évolution du deuil. Ils se sont dépris de leur identité de parents d’enfants morts de cancer. Ils ont reconnu leur ambivalence : envers la tentation euthanasique et celle de l’acharnement thérapeutique ; envers les médecins. Ils ont retrouvé leur position parentale, reconnaissant avoir répondu aux attentes de l’enfant en fin de vie ; reconnaissant le deuil intense de la fratrie. Les thèmes abordés par les parents fournissent de précieuses indications aux soignants dans les situations palliatives : aider les parents à préserver leur identité sociale, conjugale et parentale ainsi que leur présence efficace auprès de l’enfant, sans négliger la fratrie ; à exprimer leur ambivalence envers sa mort possible ; à tisser des relations de confiance réciproque avec eux.
Du sullogismos au syllogisme
Authors: Crubellier Michel 
Published: April 2011

Résumé La définition du sullogismos est strictement identique dans les Topiques et dans les Premiers Analytiques , alors qu’on admet généralement que, dans ce dernier traité, le terme désigne spécifiquement la structure formelle appelée aujourd’hui encore « syllogisme ». Le mot peut avoir le même sens d’un bout à l’autre de l’Organon et du corpus aristotélicien : il désigne le moment de la joute dialectique où l’interrogateur récapitule une section de la discussion et se montre en mesure d’imposer une conclusion à laquelle son interlocuteur ne peut pas se dérober. À la suite de Hintikka (1993), on suggère que cette notion est issue des dialogues de Platon. C’est à partir de cette conception dialectique originelle que la théorie aristotélicienne de la rhétorique, d’une part, et la « syllogistique » formelle, de l’autre, ont pu se développer.
Healthy Gaming – Video Game Design to promote Health
Authors: Fernandez-Luque L. , Tøllefsen T. , Brox E. 
Published: April 2011

Summary
Background: There is an increasing interest in health games including simulation tools, games for specific conditions, persuasive games to promote a healthy life style or exergames where physical exercise is used to control the game.
Objective: The objective of the article is to review current literature about available health games and the impact related to game design principles as well as some educational theory aspects.
Methods: Literature from the big databases and known sites with games for health has been searched to find articles about games for health purposes. The focus has been on educational games, persuasive games and exergames as well as articles describing game design principles.
Results: The medical objectives can either be a part of the game theme (intrinsic) or be totally dispatched (extrinsic), and particularly persuasive games seem to use extrinsic game design. Peer support is important, but there is only limited research on multiplayer health games. Evaluation of health games can be both medical and technical, and the focus will depend on the game purpose. Conclusion: There is still not enough evidence to conclude which design principles work for what purposes since most of the literature in health serious games does not specify design methodologies, but it seems that extrinsic methods work in persuasion. However, when designing health care games it is important to define both the target group and main objective, and then design a game accordingly using sound game design principles, but also utilizing design elements to enhance learning and persuasion. A collaboration with health professionals from an early design stage is necessary both to ensure that the content is valid and to have the game validated from a clinical viewpoint. Patients need to be involved, especially to improve usability. More research should be done on social aspects in health games, both related to learning and persuasion.
Medical Students' Participation in the 2009 Novel H1N1 Influenza Vaccination Administration: Policy Alternatives for Effective Student Utilization to Enhance Surge Capacity in Disasters
Authors: Kaiser Heather E. , Barnett Daniel J. , Hayanga Awori J. , Brown Meghan E. , Filak Andrew T. 
Published: April 2011

ABSTRACTAs cases of 2009 novel H1N1 influenza became prevalent in Cincinnati, Ohio, Hamilton County Public Health called upon the University of Cincinnati College of Medicine to enhance its surge capacity in vaccination administration. Although the collaboration was well organized, it became evident that a system should exist for medical students' involvement in disaster response and recovery efforts in advance of a disaster. Therefore, 5 policy alternatives for effective utilization of medical students in disaster-response efforts have been examined: maintaining the status quo, enhancing the Medical Reserve Corps, creating medical school–based disaster-response units, using students within another selected disaster-response organization, or devising an entirely new plan for medical students' utilization. The intent of presenting these policy alternatives is to foster a policy dialogue around creating a more formalized approach for integrating medical students into disaster surge capacity–enhancement strategies. Using medical students to supplement the current and future workforce may help substantially in achieving goals related to workforce requirements. Discussions will be necessary to translate policy into practice.(Disaster Med Public Health Preparedness. 2011;5:150–153)
Storage and Handling Guidelines for Custom Peptides
Authors: Turner Amanda , Radburn‐Smith Katy , Mushtaq Adeel , Tan Linda 
Published: April 2011

AbstractSynthetic custom peptides offer a rational, scalable, and ever more affordable approach for exploring protein‐protein interactions or even more complex phenomena, such as immune responses directed against specific epitopes. As the use of such peptides increases, it becomes increasingly important to consider all relevant factors when designing and using peptides. This unit aims to provide guidelines for the storage and handling of custom peptides, to enable the user to achieve optimal results when using peptides in their experiments. It also discusses the implications of the presence of certain amino acids in the peptide sequence and suggests design considerations to help achieve desired outcomes. Curr. Protoc. Protein Sci. 64:18.12.1‐18.12.7. © 2011 by John Wiley & Sons, Inc.
A Brief Pause between a Tagline and Brand Increases Brand Name Recognition and Preference
Authors: Mantonakis Antonia 
Published: April 2011

SummaryThis paper explores the outcome of the visual encoding of brands in meaningful sentences (i.e. in taglines) on brand name recognition and preference. In this paper, it is shown that, above and beyond the role of conceptual priming during encoding at increasing recognition memory, there is a role of creating a temporal delay, or pause, between meaningful cues in the sentence and a key word (Experiment 1) or brand (Experiments 2a and 3) on memory. The pause is also associated with increased preference towards brands (Experiment 2b). These findings demonstrate a new way to enhance recognition of brand names that is not due to a pure generation effect but rather by increasing attention, which increases processing fluency of the target. Copyright © 2011 John Wiley & Sons, Ltd.
Charge‐Separation Dynamics in Inorganic–Organic Ternary Blends for Efficient Infrared Photodiodes
Authors: Jarzab Dorota , Szendrei Krisztina , Yarema Maksym , Pichler Stefan , Heiss Wolfgang , Loi Maria A. 
Published: April 2011

AbstractKnowledge about the working mechanism of the PbS:P3HT:PCBM [P3HT=poly(3‐hexylthiophene), PCBM=[6,6]‐phenyl‐C61 ‐butyric acid methyl ester] hybrid blend used for efficient near‐infrared photodiodes is obtained from time‐resolved photoluminescence (PL) studies. To understand the role of each component in the heterojunction, the PL dynamics of the ternary (PbS:P3HT:PCBM) blend and the binary (PbS:P3HT, PbS:PCBM and P3HT:PCBM) blends are compared with the PL of the pristine PbS nanocrystals (NCs) and P3HT. In the ternary blend the efficiency of the charge transfer is significantly enhanced compared to the one of PbS:P3HT and PbS:PCBM blends, indicating that both hole and electron transfer from excited NCs to the polymer and fullerene occur. The hole transfer towards the P3HT determines the equilibration of their population in the NCs after the electron transfer towards PCBM, allowing their re‐excitation and new charge transfer process.
Permanent and Peripheral Embolization: Temperature‐Sensitive p(N‐Isopropylacrylamide‐co‐butyl Methylacrylate) Nanogel as a Novel Blood‐Vessel‐Embolic Material in the Interventional Therapy of Liver Tumors
Authors: Zhao Yanbing , Zheng Chuansheng , Wang Qin , Fang Jianlin , Zhou Guofeng , Zhao Hui , Yang Yajiang , Xu Huibi , Feng Gansheng , Yang Xiangliang 
Published: April 2011

AbstractPermanent and peripheral embolization is a requirement of embolic materials in the transcatheter arterial embolization of liver tumors. So far, it has been difficult to find materials that have both good flowability and high mechanical strength. In the present work, a temperature‐sensitive p(N ‐isopropylacrylamide‐co‐butyl methylacrylate) (PIB) nanogel is explored as a novel blood‐vessel‐embolic material in the interventional therapy of liver tumors. With increasing temperature, the PIB nanogel dispersion sequentially exhibits three phase states; swollen gel, flowable sol, and finally shrunken gel. Iohexol, a nonionic X‐ray contrast agent, increases the volume‐phase transition temperature of the PIB nanogel and decreases the critical gelation concentration. Angiographical and histological studies on the embolization in the liver arteries of VX2 tumor‐bearing rabbits indicate that the PIB nanogel dispersion mixed with iohexol (designated as PIB‐I‐6150) completely occludes all levels of blood vessels, including peripheral vessels. In addition, data on tumor volume, necrosis level, and the number of metastatic foci indicate that PIB‐I‐6150 has better peripheral embolization than Lipiodol. Experiments concerning cytotoxicity, hemolysis, histology, and liver function indicate that PIB‐I‐6150 has good biocompability.
Surface Domain Structures and Mesoscopic Phase Transition in Relaxor Ferroelectrics
Authors: Kholkin Andrei , Morozovska Anna , Kiselev Dmitry , Bdikin Igor , Rodriguez Brian , Wu Pingping , Bokov Alexei , Ye Zuo‐Guang , Dkhil Brahim , Chen Long‐Qing , Kosec Marija , Kalinin Sergei V. 
Published: April 2011

AbstractRelaxor ferroelectrics are a prototypical example of ferroic systems in which interplay between atomic disorder and order parameters gives rise to emergence of unusual properties, including non‐exponential relaxations, memory effects, polarization rotations, and broad spectrum of bias‐ and temperature‐induced phase transitions. Despite more than 40 years of extensive research following the original discovery of ferroelectric relaxors by the Smolensky group, the most basic aspect of these materials – the existence and nature of order parameter – has not been understood thoroughly. Using extensive imaging and spectroscopic studies by variable‐temperature and time resolved piezoresponse force microscopy, we find that the observed mesoscopic behavior is consistent with the presence of two effective order parameters describing dynamic and static parts of polarization, respectively. The static component gives rise to rich spatially ordered systems on the ∼100 nm length scales, and are only weakly responsive to electric field. The surface of relaxors undergoes a mesoscopic symmetry breaking leading to the freezing of polarization fluctuations and shift of corresponding transition temperature.
The Potential of Perylene Bisimide Derivatives for the Solubilization of Carbon Nanotubes and Graphene
Authors: Backes Claudia , Hauke Frank , Hirsch Andreas 
Published: April 2011

AbstractCarbon nanotubes and graphene are outstanding materials of the 21st century with a broad spectrum of applications. However, major challenges are faced such as the intrinsically low solubility of both sp2 carbon allotropes. To overcome this hurdle the potential of noncovalent functionalization is summarized with a special focus on the establishment of the perylene bisimide unit as aromatic anchor to the graphitic surface. Rational surfactant design is unmasked as the key to solubilization of the carbon allotropes, while at the same time tailoring their surface properties, or even electronic properties in a fully reversible fashion.
A study of dimensional, swelling, and gel changes in hydrophilic and hydrophobic polymer mixtures of chitosan and their possible use as carriers for sustained release drug delivery
Authors: Efentakis M. , Stamoylis K. 
Published: April 2011

AbstractThe aim of this investigation was to study and estimate the dimensional changes, gel evolution, swelling, and erosion of chitosan lactate mixture tablets with Polyox or Ethocel, in distilled water and 0.1 N hydrochloric acid (HCI) solution, using an image analysis method. This method permits the accurate measurement and evaluation of dimensional changes, core changes, and gel growth. All tablets displayed greater dimensional expansion in water than in HCI. The fastest and greatest swelling was exhibited in water, whereas, in contrast, the greatest erosion was observed in HCI. Chitosan/Polyox tablets exhibited greater expansion, swelling, and gel growth than the equivalent chitosan/Ethocel tablets. The thickness of the gel layer in chitosan/Polyox tablets, particularly, increased considerably with time up to 8 h, indicating that the development of a thick and durable gel barrier is a characteristic necessity for the preparation of sustained drug release delivery systems. In addition, the differentiation of the above‐mentioned characteristics in relation to changes in pH values also gives useful information about sustained release preparations made from these polymers, since it is well known that the gastrointestinal tract is characterized by a range of pH values. In general, the polymer mixtures studied comprise a useful and promising combination of materials for the development and manufacturing of sustained release devices based on these polymers. © 2011 Wiley Periodicals, Inc. Adv Polym Techn 30: 110–121, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/adv.20208
Microwave‐enhanced synthesis of graft copolymer of binary vinyl monomer mixtures onto acetylated Saccharum spontaneum L and characterization
Authors: Maiti Mithu , Kaith Balbir Singh , Jindal Rajeev , Jana Asim K. 
Published: April 2011

AbstractSynthesis of graft copolymer under the influence of microwave radiation is a rapid, efficient, clean, cheap, convenient, energy‐saving, and green method. Grafting of methylmethacrylate (MMA) + acrylamide (AAm), MMA + acrylonitrile (AN), and MMA + acrylic acid (AA) binary mixtures onto acetylated Saccharum spontaneum L was carried out under the influence of microwave radiation (MWR), and maximum graft yield 94.5% was found with MMA + AAm binary mixture. Synthesized graft copolymers were characterized with FT‐IR, scanning electron microscopy, TGA/DTA/DTG, and X‐ray diffraction techniques. Thermal stability of Ss‐g‐poly(MMA + AAm) was found to be more than that of acetylated S. spontaneum fiber and other graft copolymers. Although on grafting, percentage crystallinity and crystallinity index decreased but graft copolymers were found to exhibit more moisture, chemical, and thermal resistance. © 2011 Wiley Periodicals, Inc. Adv Polym Techn 30: 122–137, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/adv.20209
Dynamic rheological behavior and morphology of polylactide/poly(butylenes adipate‐co‐terephthalate) blends with various composition ratios
Authors: Li Ke , Peng Jun , Turng Lih‐Sheng , Huang Han‐Xiong 
Published: April 2011

AbstractPolylactide (PLA)/poly(butylenes adipate‐co‐terephthalate) (PBAT) blends with various composition ratios were prepared via melt compounding using a twin‐screw extruder. The dynamic rheological behavior and morphology of the blends were studied using a parallel‐plate rheometer and a scanning electron microscope (SEM), respectively. The results showed that the shear‐thinning tendency of blends became stronger with increasing PBAT content. Moreover, at low frequencies, the complex viscosity, storage modulus and loss modulus for blends with 20 wt% PBAT content were higher than that of other samples when PBAT content was less than 50 wt%. Based on SEM images, three distinct morphologies—spherical droplet, elongated fibrous structure, and co‐continuous structure—were observed as the content of PBAT increased from 5 to 50 wt%. When the PBAT content reached 70 wt% and above, the morphology became droplets again, with PLA droplets dispersing within the PBAT matrix. In addition, the PLA droplets in the PBAT/PLA (80/20 w/w) blend were smaller and more regular than the PBAT droplets in the PLA/PBAT (80/20 w/w) blend, despite the same composition ratio. © 2011 Wiley Periodicals, Inc. Adv Polym Techn 30: 150–157, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/adv.20212