bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of periodontal health status in patients with Parkinson's disease—A systematic review and meta‐analysis of cross‐sectional studies
Authors: Suryawanshi Krishna R. , Martande Santosh , Kalal Darshan N. , PV Swathi , Gopalakrishnan Dharmarajan , Kulloli Anita , Shetty Sharath , Thakur Shambhavi , Rokade Shreya , Thomas Jonathan 
Published: April 2024

AbstractIntroductionParkinson's disease (PKD) is neurodegenerative disorder marked by tremors, bradykinesia, muscle rigidity and reduction in precise hand movements which could lead to improper oral hygiene and Periodontal disease. Current systematic review aims to review existing literature and provide assessment of periodontal health in PKD patients through a meta‐analysisMethodsReview was performed in accordance with Preferred Reporting Items for Systematic Reviews and Meta‐Analysis (PRISMA) guidelines and registered in PROSPERO—CRD42023451700. Databases were searched for studies having sufficient data on periodontal health in patients with PKD compared to healthy controls and reporting outcomes in terms of various periodontal parameters of probing depth (PD), plaque index (PI), clinical attachment level (CAL), presence of bleeding on probing and gingivitis. Quality assessment of included was evaluated using Newcastle Ottawa Scale (NOS).ResultsEleven studies fulfilled the eligibility criteria, of which ten studies were suitable for meta‐analysis. Pooled estimate through the SMD showed that all periodontal parameters were altered and significantly deteriorated in PKD compared to controls but this group differences were statistically insignificant (p > 0.05). Publication bias through the funnel plot showed symmetric distribution with absence of systematic heterogeneity.ConclusionParkinson's disease could be a possible factor in deterioration of periodontal health.
Primary microfossiliferous chert in the Aptian Barra Velha Formation
Authors: Moore Kelsey R. , Crémière Antoine , Present Theodore M. , Barnett Andrew , Bergmann Kristin D. , Amthor Joachim , Grotzinger John 
Published: April 2024

ABSTRACTThe Barra Velha Formation and other Aptian pre‐salt deposits record the history of the proto‐Atlantic basin and the rifting of Gondwana. Studies have sought to characterize the depositional environment of the basin with a focus on carbonate fabrics and magnesium silicate clays. However, the water chemistry and fluid sources in the basin, the silica cycle, and how the basin evolved over time are not fully constrained. Additionally, current understanding of the microbiota that inhabited this basin is incomplete because microfossils have rarely been identified in pre‐salt deposits, especially on the Brazilian margin. This study describes authigenic chert in the Barra Velha Formation that preserves distinct, organic‐rich structures and textures. The petrographic relationships between the chert and carbonate suggest that both formed as authigenic phases, but their formation was temporally decoupled. These relationships and δ30Si and δ18O data suggest that chert post‐dates the formation and subsequent dissolution of the carbonates, and may have formed from a different fluid. By characterizing the chert–carbonate paragenesis and mechanism of chert formation, this study provides new insights into the fluid sources and complexity of the basin. Together, the results of this research suggest that the chert precipitated as primary, authigenic phases after karstification of the carbonate from a newly introduced, low temperature, freshwater fluid that was chemically distinct from the lake water. The chert preserves organic matter that is compositionally and texturally distinct from the void‐filling bitumen associated with the classically studied carbonate facies. Based on the composition and morphologies of organic structures, this is likely primary organic matter and a morphologically diverse microfossil assemblage preserved in place at the time of chert formation. Thus, this early chert provides new insights into the water chemistry, fluid sources and silica cycle in the basin, and represents a unique taphonomic window that helps us characterize the pre‐salt basin microbiota.
Classical and team reasoning in the Centipede Game
Authors: Sklar David 
Published: April 2024

AbstractThis study analyses behaviour in non‐zero‐sum finite multi‐stage games, particularly the Centipede Game. The classical Nash Equilibrium fails to explain empirical behaviour and intuitive logic and has therefore been challenged. This paper introduces the ‘Pure Collective Equilibrium’, or PCE, which describes the equilibrium reached when agents assess their utility not by their own payoffs but by the mean collective payoff of the team, as outlined by some team‐reasoning hypotheses. Classical behaviour and purely collective team reasoning then both represent special cases—the first in which agents and their counterparts are on the Nash Equilibrium path, and the second in which agents and their counterparts are purely collective team reasoners. It is argued that agents intentionally and consistently deviate from classical rationality in the Centipede Game, even in the absence of philanthropic altruists or error‐plagued populations.
Burkholderia gladioli deep pyoderma in a dog secondary to immunosuppressive ciclosporin and prednisone therapy
Authors: Rosenkrantz Wayne , Ritter Jana M. , Keating M. Kelly , Bhatnagar Julu , Krumbeck Janina A. 
Published: April 2024

AbstractA dog presented with deep pyoderma on the paw, following treatment with ciclosporin and prednisone for immune‐mediated haemolytic anaemia. Cytological evaluation, skin biopsy, aerobic culture, next‐generation DNA sequencing and PCR were used to detect the first reported case of Burkholderia gladioli in a dog.
Teleconsulting in wound care: Connecting the primary care to the wound specialist reduces unnecessary referrals
Authors: Brekelmans W. , van der Burg B. L. S. Borger , Brouwer R. J. , Belo J. N. , Hoencamp R. 
Published: April 2024

AbstractIn the Netherlands the primary care (General Practitioner or homecare nurse) encounter a variety of wounds ranging from traumatic to diabetic foot ulcers. According to a recent study 82.4% of the patients with a wound can be treated in a primary setting with the GP as medical supervisor. The remaining 17.6% of patients need more extensive care including advice by a specialised doctor, diagnosis and treatment. Prompt analyses and treatment of underlying causes by specialised doctors in a multidisciplinary setting is necessary for treating patients with complicated wound. This article describes the impact of Electronic Health Consultation on all wounds treated in the primary care. And describes the effect on the duration until referral to the hospital and the influence on the amount of unnecessary referrals to the hospital. All data was collected prospectively from June 2020 until October 2021. The study involved a process where primary care could seek advice from a Wound Physician at the Alrijne Wound Centre through a specialised Electronic Health Consultation. A total of 118 patients were analysed. 41/118 (34.7%) patients required a physical consultation with analysis and treatment in the hospital, after teleconsultation. The remaining 77/118 (65.3%) could be treated in primary care after Electronic Health Consultation. The mean duration of wound existence until Electronic Health Consultation was 39.3 days (range 5–271, SD: 38.5). 3/41 (7.3%) of the referrals were unnecessary. Electronic Health Consultation serves as a valuable and efficient tool for enhancing wound care, ultimately contributing to improved patient management and resource allocation within the healthcare system. This article describes the impact of Electronic Health Consultation on all wounds treated in the primary care and the influence on the duration until referral to the hospital and the influence on the amount of unnecessary referrals to the hospital.
Dysregulation of miR‐146a is associated with exacerbated inflammation, oxidative and endoplasmic reticulum stress in the progression of diabetic foot ulcer
Authors: Vikraman Pooja Prathyushaa , Amin Karan , Mohandas Sundhar , Umapathy Dhamodharan , Kesavan Rajesh , Ramkumar Kunka Mohanram 
Published: April 2024

AbstractRecent evidence has implicated the role of microRNA‐146a (miR‐146a) in regulating inflammatory responses. In the present study, we investigated the role of miRNA‐146a in the progression of diabetic foot ulcer (DFU) in type 2 diabetes mellitus patients (T2DM) and studied its correlation with stress mediators such as Endoplasmic Reticulum (ER) and oxidative stress. Ninety subjects were enrolled and evenly distributed among three groups: Controls (n = 30), T2DM without complications (n = 30) and T2DM with foot ulcers (n = 30). Subsequently, each group was further subdivided based on the University of Texas classification. Peripheral blood was collected from all the study subjects, while tissue biopsies were taken only from DFU patients. Total RNA from both PBMCs and wound tissues were isolated using miRNA isolation kit and qPCR was performed to check the expression of miR‐146a, ER stress and oxidative stress markers. Our findings revealed a significant decrease in miR‐146a expression among T2DM patients with Grade 2 and Grade 3 DFUs compared with those with Grade 0 and Grade 1 DFUs. Notably, inflammatory genes regulated by miR‐146a, including TRAF6, IRAK‐1 and ADAM, were all upregulated in T2DM patients with Grade 2 and Grade 3 DFUs. Moreover, reduced miR‐146a levels were correlated with increased markers of ER stress and oxidative stress in Grade 2 and Grade 3 DFU patients. Furthermore, our in vitro experiment using mouse 3T3 fibroblasts demonstrated a downregulation of miR‐146a following induction of hyperglycaemia, ER stress and oxidative stress in these cells. These findings suggest a potential link between diminished miR‐146a expression and heightened oxidative and ER stress in T2DM patients with more severe grades of DFUs. Our results imply that targeting miR‐146a may hold therapeutic promise for managing disease progression in DFU patients, as it could help alleviate oxidative and ER stress associated with diabetic complications.
Multimodal Convolutional Neural Networks for Sperm Motility and Concentration Predictions
Authors: Goh Voon Hueh , Mansor Muhammad Asraf , As'ari Muhammad Amir , Ismail Lukman Hakim 
Published: April 2024

Semen analysis is an important analysis for male infertility primary investigation and manual semen analysis is a conventional method to assess it. Manual semen analysis has been revealed with accuracy and precision limitations due to noncompliance to guidelines and procedures. Sperm motility and concentration are the main indicators for pregnancy and conception rate hence they were selected for parameters prediction. Convolutional neural network (CNN) has benefited computer vision application industry in recent years and has been widely applied in computer vision research tasks. In this paper, three-dimensional CNN (3DCNN) was designed to extract motion and temporal features, which are vital for sperm motility prediction. For sperm concentration, since two-dimensional CNN (2DCNN) is efficient in recognizing and extracting spatial features, well-established Residual Network (ResNet) architecture was adopted and customized for sperm concentration prediction. Multimodal learning approach is a technique to aggregate learnt features from different deep learning architecture that adopted other forms of modalities, which could provide deep learning model with better insights on their tasks. Hence, a multimodal learning deep learning architecture was designed to receive both image-based (frames extracted from video samples) and video-based (stacked frames pre-processed from video samples) input that could provide well-extracted spatial and temporal features for sperm parameters prediction. The results obtained using the proposed methodology have surpassed other similar research works who used deep learning approach. For sperm motility, its best achieved average mean absolute error (MAE) was 8.048, and sperm concentration obtained a competent Pearson’s correlation coefficient (RP) value of 0.853.
Impact of Aluminum Oxide & Silicon Dioxide on Nanofluid Flow Over a Stretching Sheet with Heat Transfer: Analytical Solution
Authors: Noranuar Wan Nura'in Nabilah , Mohamad Ahmad Qushairi , Jiann Lim Yeou , Shafie Sharidan , Jamaludin Mohd Anuar 
Published: April 2024

A stretchable surface is one of the products features that numerous industrial and engineering field has been taken into consideration due to of its benefit. However, most of the fluid mechanic simulation for stretchable surface has been solved numerically and there is very limited theoretical study discovering this problem. Therefore, the present study investigated the convective Casson nanofluid flow and heat transfer over a linear stretching sheet. The aluminum oxide and silicon dioxide are considered. The analytical resolution of the governing problem yields velocity and temperature solutions using the Laplace transform method. Graphical representation illustrates how nanoparticle volume fraction affects velocity and temperature distribution profiles. Higher nanoparticle volume fractions slow down nanofluid flow and elevate temperature profiles. This investigation establishes a robust foundation for future research utilizing numerical methods.
Mapping simulation-based activities for health professionals in rural and remote contexts in high-income countries: a scoping review protocol
Authors: Smith Naomi Tarus , Muller Spiti Julia , Padley James , Davies Ellen 
Published: April 2024

Objective:
This scoping review will aim to map the existing academic literature on simulation-based activities that are designed with and delivered for health professionals in geographically rural and remote contexts in high-income countries.

Introduction:
Simulation-based health care activities are implemented in health services to increase patient safety because they allow health professionals to prepare, learn, practice, rehearse, and improve clinical performance and teamwork. Simulation-based activities are increasingly being used in rural and remote areas, where resources are limited compared with metropolitan areas.

Inclusion criteria:
This review will include all primary and peer-reviewed research articles and abstracts that report health simulation methodologies and activities that have been used in rural and remote health care contexts in high-income countries.

Methods:
The proposed review will follow the JBI guidelines for scoping reviews and the Preferred Reporting Items for Systematic Reviews and Meta-Analysis extension for Scoping Reviews (PRISMA-ScR) guidelines. Databases to be searched will include Embase, Emcare, and MEDLINE (all searched via Ovid), Scopus, PubMed, and the Cochrane Database. Titles and abstracts will be screened independently by 2 reviewers, followed by full-text screening. Data will be extracted and analyzed to address the review questions.

Review registration:
Open Science Framework https://osf.io/pu6gv
Loureirin A is a narrow-spectrum antimicrobial agent against
Helicobacter pylori
Authors: Tong Qian , Yin Chengqiang , Hang Xudong , Bai Yuefan , Zhang Chongwen , Xu Jingcheng , Huang Yan , Ge Yixin , Chen Tianyu , Zeng Liping , Jia Jia , Bi Hongkai 
Published: April 2024

ABSTRACT

Currently,
Helicobacter pylori
eradication by antibiotic therapy faces various challenges, including antibiotic resistance, side effects on intestinal commensal bacteria, and patient compliance. In this study, loureirin A (LrA), a traditional Chinese medicine monomer extracted from Sanguis Draconis flavones, was found to possess specific antibacterial activity against
H. pylori
without the bacteria displaying a tendency to develop resistance
in vitro
. LrA demonstrated a synergistic or additive effect when combined with omeprazole (a proton pump inhibitor) against
H. pylori
. The combination of LrA and omeprazole showed promising anti-
H
.
pylori
potential, exhibiting notable
in vivo
efficacy comparable to standard triple therapy in mouse models infected with both drug-sensitive and drug-resistant
H. pylori
strains. Moreover, the narrow-spectrum antibacterial profile of LrA is reflected in its minimal effect on the diversity and composition of the mouse gut microbiota. The underlying mechanism of action of LrA against
H. pylori
involves the generation of bactericidal levels of reactive oxygen species, resulting in apoptosis-like cell death. These findings indicate that LrA is a promising lead compound targeting
H. pylori
without harming the commensal bacteria.
Gut microbiota intervention alleviates pulmonary inflammation in broilers exposed to fine particulate matter from broiler house
Authors: Liu Junze , Li Yuan , Shen Dan , Li Xiaoqing , Wang Kai , Nagaoka Kentaro , Li Chunmei 
Published: April 2024

ABSTRACT

The gut microbiota of poultry is influenced by a variety of factors, including feed, drinking water, airborne dust, and footpads, among others. Gut microbiota can affect the immune reaction and inflammation in the lungs. To investigate the effect of gut microbiota variation on lung inflammation induced by PM
2.5
(fine particulate matter) in broilers, 36 Arbor Acres (AA) broilers were randomly assigned to three groups: control group (CON), PM
2.5
exposure group (PM), and PM
2.5
exposure plus oral antibiotics group (PMA). We used non-absorbable antibiotics (ABX: neomycin and amikacin) to modify the microbiota composition in the PMA group. The intervention was conducted from the 18th to the 28th day of age. Broilers in the PM and PMA groups were exposed to PM by a systemic exposure method from 21 to 28 days old, and the concentration of PM
2.5
was controlled at 2 mg/m
3
. At 28 days old, the lung injury score, relative mRNA expression of inflammatory factors, T-cell differentiation, and dendritic cell function were significantly increased in the PM group compared to the CON group, and those of the PMA group were significantly decreased compared to the PM group. There were significant differences in both α and β diversity of cecal microbiota among these three groups. Numerous bacterial genera showed significant differences in relative abundance among the three groups. In conclusion, gut microbiota could affect PM
2.5
-induced lung inflammation in broilers by adjusting the capacity of antigen-presenting cells to activate T-cell differentiation.

IMPORTANCE
Gut microbes can influence the development of lung inflammation, and fine particulate matter collected from broiler houses can lead to lung inflammation in broilers. In this study, we explored the effect of gut microbes modified by intestinal non-absorbable antibiotics on particulate matter-induced lung inflammation. The results showed that modification in the composition of gut microbiota could alleviate lung inflammation by attenuating the ability of dendritic cells to stimulate T-cell differentiation, which provides a new way to protect lung health in poultry farms.
Deciphering
Staphylococcus aureus–
host dynamics using dual activity-based protein profiling of ATP-interacting proteins
Authors: Ahator Stephen Dela , Hegstad Kristin , Lentz Christian S. , Johannessen Mona 
Published: April 2024

ABSTRACT

The utilization of ATP within cells plays a fundamental role in cellular processes that are essential for the regulation of host–pathogen dynamics and the subsequent immune response. This study focuses on ATP-binding proteins to dissect the complex interplay between
Staphylococcus aureus
and human cells, particularly macrophages (THP-1) and keratinocytes (HaCaT), during an intracellular infection. A snapshot of the various protein activity and function is provided using a desthiobiotin-ATP probe, which targets ATP-interacting proteins. In
S. aureus
, we observe enrichment in pathways required for nutrient acquisition, biosynthesis and metabolism of amino acids, and energy metabolism when located inside human cells. Additionally, the direct profiling of the protein activity revealed specific adaptations of
S. aureus
to the keratinocytes and macrophages. Mapping the differentially activated proteins to biochemical pathways in the human cells with intracellular bacteria revealed cell-type-specific adaptations to bacterial challenges where THP-1 cells prioritized immune defenses, autophagic cell death, and inflammation. In contrast, HaCaT cells emphasized barrier integrity and immune activation. We also observe bacterial modulation of host processes and metabolic shifts. These findings offer valuable insights into the dynamics of
S. aureus
–host cell interactions, shedding light on modulating host immune responses to
S. aureus
, which could involve developing immunomodulatory therapies.

IMPORTANCE

This study uses a chemoproteomic approach to target active ATP-interacting proteins and examines the dynamic proteomic interactions between
Staphylococcus aureus
and human cell lines THP-1 and HaCaT. It uncovers the distinct responses of macrophages and keratinocytes during bacterial infection.
S. aureus
demonstrated a tailored response to the intracellular environment of each cell type and adaptation during exposure to professional and non-professional phagocytes. It also highlights strategies employed by
S. aureus
to persist within host cells. This study offers significant insights into the human cell response to
S. aureus
infection, illuminating the complex proteomic shifts that underlie the defense mechanisms of macrophages and keratinocytes. Notably, the study underscores the nuanced interplay between the host’s metabolic reprogramming and immune strategy, suggesting potential therapeutic targets for enhancing host defense and inhibiting bacterial survival. The findings enhance our understanding of host–pathogen interactions and can inform the development of targeted therapies against
S. aureus
infections.