bluuebunny/crossref_metadata_2025_split huggingface dataset:
Densities of the Vulnerable marsh deer Blastocerus dichotomus in Bolivia's northern savannahs
Authors: Ríos-Uzeda Boris , Mourão Guilherme 
Published: April 2012

AbstractAerial surveys have been used successfully to estimate vertebrate populations in open habitats. The marsh deer Blastocerus dichotomus, categorized as Vulnerable on the IUCN Red List, lives in such habitats and is suitable for aerial counting because it is conspicuous. This species, the largest South American deer, is native to Argentina, Boliva, Brazil, Paraguay and Peru but no reliable information has previously been available on its populations in Bolivia. From May to August 2007 we conducted aerial transects to survey marsh deer in three large areas of savannah. We used a modified mark–recapture method to improve the accuracy of the counts and estimated density and abundance. The corrected, estimated density of the marsh deer was 0.24 km−2 in the northern La Paz Department, 0.12 km−2 in Mamoré and 0.15 km−2 in Iténez. These densities are similar to the mean density of the species on other South American savannahs. This is the first large-scale survey of the marsh deer in Bolivia and the first to provide information about the density of the species in the Amazon. We recommend the creation of protected areas in these savannahs, and wildlife and domestic health programmes to conserve the marsh deer of this region.
Integrating family planning service provision into community-based marine conservation
Authors: Harris Alasdair , Mohan Vik , Flanagan Maggie , Hill Rebecca 
Published: April 2012

AbstractHuman population growth is one of the primary drivers of biodiversity loss. Throughout much of the developing world growth of human populations is occurring in part as a result of a lack of access to sexual and reproductive health services, and this is having profoundly negative impacts on biodiversity and natural resource-dependent livelihoods. We present experiences of the incorporation of sexual and reproductive health services within a pre-existing community-based marine conservation initiative in Madagascar as part of an integrated population, health and environment (PHE) programme. Our results demonstrate the considerable demand for, and lack of social barriers to, the introduction of sexual and reproductive health services in this region. These findings emphasize the mutually beneficial synergies, supporting both public health and conservation objectives, which can be created by integrating sexual and reproductive health services into more conventional biodiversity conservation activities. This PHE approach demonstrates the inextricable link between reproductive health and resource use by providing practical, immediate and lasting benefits to public health, gender equity, food security and biodiversity conservation.
Mixed choruses and marriage songs: a new interpretation of the third stasimon of the Hippolytos
Authors: Swift L.A. 
Published: April 2012

AbstractThis article uses evidence drawn from hymenaios and wedding ritual to reach a new interpretation of the third stasimon of the Hippolytos, and its rôle in the play. There is longstanding contention about whether a second (male) chorus participates in the ode, singing in antiphony with the existing tragic chorus. Even scholars who accept that a second chorus is present have tended to regard it as an aberration which needs to be explained away, rather than a deliberate choice with poetic significance. I discuss the cultural implications of such a chorus, examining our evidence for real-life mixed choruses, and then applying this to the ode itself. The evidence for mixed choruses suggests they are strongly associated with marriage. Looking more closely at the language and imagery of the ode, there are allusions to the topoi of wedding songs and ritual running through it. The ode can use these as a device to trigger deep-rooted responses and associations from the audience, as these motifs are drawn from the cultural tradition which the audience shares. The topoi tie in with the theme of marriage and sexuality within the Hippolytos as a whole. But while their usual purpose is to set up conventional models and ways of thinking, the way they are deployed in the ode in fact serves to undermine these models, and to put a darker spin on the norms of sexual behaviour. This strand of imagery therefore also provides a filter for interpreting Hippolytos' own attitude towards sexuality, and a guide to how we are meant to respond to it.
Bayesian prediction of geomagnetic storms: Wind data, 1996–2010
Authors: Chen James , Slinker Steven P. , Triandaf Ioana 
Published: April 2012

The feature‐based Bayesian method previously developed by Chen et al. (1996, 1997) to predict the occurrence, severity, and duration of large geomagnetic storms has been run on a daily basis on the Wind/Magnetic Fields Investigation (MFI) data from January 1996 until March 2010. The algorithm uses as input real‐time solar wind magnetic field data obtained at the L1 Lagrange point, and the output is the probability prediction of the magnetic field structure of the upstream solar wind that has yet to arrive, and its geoeffectiveness, where geoeffectiveness is measured by the traditional Dst index. The performance characteristics of the method are evaluated using a four‐level contingency table: nonstorm disturbances (−80 nT < Dst ≤ − 50 nT), weak storms (−120 nT < Dst ≤ − 80 nT), moderate storms (−160 nT < Dst ≤ − 120 nT), and strong storms (Dst ≤ − 160 nT). It is found that the greater the level of disturbances, the more accurate the prediction is. With moderate and strong storms combined, the algorithm correctly predicted 30 out of 37 storms (81%). The false negatives are caused by solar wind structures with short durations (≲ 1–2 hrs) of strong southward magnetic field (say, Bz ≲ −30 nT), which are sparsely represented in the probability distribution functions constructed using prior solar wind data (OMNI data set from 1973–1981). The algorithm does not predict the storm onset time, but the results of the present and previous tests show that the average warning time ranges from a few hours to a maximum of 10–15 hours.
Lithium-ion conducting La2/3−xLi3xTiO3 solid electrolyte thin films with stepped and terraced surfaces
Authors: Ohta Hiromichi , Mizoguchi Teruyasu , Aoki Noriyuki , Yamamoto Takashi , Sabarudin Akhmad , Umemura Tomonari 
Published: April 2012

La2/3−xLi3xTiO3 (LLT, x = 0.1 and 0.167) thin films were grown on (001)-oriented SrTiO3 (STO) substrates by pulsed laser deposition. The resultant LLT films were heteroepitaxially grown with atomically flat surfaces. Although the films considered to be single crystalline, they were composed of 90°-rotated multi domains (∼5 nm in size) with epitaxial relationships of (100) [001] LLT || (001) [100] STO and (100) [010] LLT || (001) [100] STO. The bulk conductivity of the LLT (x = 0.1) films was 2.5 × 10−2 S cm−1 at 190 °C, comparable to that of a bulk single crystalline LLT. Single crystalline LLT thin films with atomically flat surfaces should help clarify interfacial lithium-ion conductivity.
At the request of the authors, this article is being retracted effective 1 March 2013.
THE RACIALIZATION OF PUBLIC DISCOURSE
Authors: Padovan Dario , Alietti Alfredo 
Published: April 2012

ABSTRACT
The objective of this research is to understand the nature and diffusion of antisemitism and Islamophobia in Italy. It is based on 1528 interviews among a representative sample of the Italian population stratified according to age, gender, and residence. The questionnaire used was built by a set of different scales: antisemitic, Islamophobic, and three others scales to take into account authoritarian, ethnocentric, and anomic attitudes. To test assumptions regarding the impact of these predictors of antisemitic and Islamophobic prejudice, it was decided to adopt the statistical technique of path analysis. Outcomes of our research draw attention on the distribution of antisemitism and Islamophobia among Italian people, their nature as a combination of old and new prejudices, and their linking with broader sets of attitudes such as anomie, ethnocentrism, and authoritarianism.
Accurate extension of multiple sequence alignments using a phylogeny-aware graph algorithm
Authors: Löytynoja Ari , Vilella Albert J. , Goldman Nick 
Published: April 2012

Abstract
Motivation: Accurate alignment of large numbers of sequences is demanding and the computational burden is further increased by downstream analyses depending on these alignments. With the abundance of sequence data, an integrative approach of adding new sequences to existing alignments without their full re-computation and maintaining the relative matching of existing sequences is an attractive option. Another current challenge is the extension of reference alignments with fragmented sequences, as those coming from next-generation metagenomics, that contain relatively little information. Widely used methods for alignment extension are based on profile representation of reference sequences. These do not incorporate and use phylogenetic information and are affected by the composition of the reference alignment and the phylogenetic positions of query sequences.
Results: We have developed a method for phylogeny-aware alignment of partial-order sequence graphs and apply it here to the extension of alignments with new data. Our new method, called PAGAN, infers ancestral sequences for the reference alignment and adds new sequences in their phylogenetic context, either to predefined positions or by finding the best placement for sequences of unknown origin. Unlike profile-based alternatives, PAGAN considers the phylogenetic relatedness of the sequences and is not affected by inclusion of more diverged sequences in the reference set. Our analyses show that PAGAN outperforms alternative methods for alignment extension and provides superior accuracy for both DNA and protein data, the improvement being especially large for fragmented sequences. Moreover, PAGAN-generated alignments of noisy next-generation sequencing (NGS) sequences are accurate enough for the use of RNA-seq data in evolutionary analyses.
Availability: PAGAN is written in C++, licensed under the GPL and its source code is available at http://code.google.com/p/pagan-msa.
Contact: ari.loytynoja@helsinki.fi
Supplementary information: Supplementary data are available at Bioinformatics online.
First Report Regarding Potato Scab Caused by Streptomyces acidiscabies in Uruguay
Authors: Lapaz M. I. , Verdier E. , Pianzzola M. J. 
Published: April 2012

Potato scab disease is caused by gram-positive filamentous bacteria in the genus Streptomyces. A great variety of species cause this disease, but Streptomyces scabies is the most ancient of these pathogens and can be found in a worldwide distribution, whereas S. turgidiscabies and S. acidiscabies are newly emerged pathogens (2). During the autumn of 2010, potato (Solanum tuberosum) crops had large economic losses by common scab, corresponding to 29% of the total potato-cultivation area (according to our survey), which was unusual in Uruguay. Specifically, the disease was very aggressive and the tubers showed particularly deep scab lesions. We isolated the Streptomyces species present in these particular scab lesions of tubers collected in July 2010 from one of the three potato cultivation areas (San José). A total of 19 Streptomyces spp. strains were isolated and identified using classical and molecular techniques. Morphological characteristics of colonies and microscopic structure of the mycelium were observed (1). Molecular characterization by conventional PCR was carried out using primers directed to specific regions of the 16S rRNA gene for the genus Streptomyces, Aci1: (5′-TCACTCCTGCCTGCATGGGCG-3′) and Aci2: (5′-CGACAGCTCCCTCCCACAAG-3′). Also, regions of two pathogenicity genes, namely txtAB and nec1, were amplified and confirmed by sequencing (2). Additionally, melanin production and pathogenicity of the isolates was determined by inoculation of potato discs (1). Six of the 19 strains succeeded in PCR amplification with primers specific to Streptomyces acidiscabies, which has white, aerial hypha and flexuous spore chains. These strains did not produce melanin on tyrosine agar media. The amplified fragments for 16S rRNA and pathogenicity genes from one representative strain 61 were sequenced. BLASTn analysis of the 16S rRNA gene sequence obtained of the strain 61 (Accession No. JN206667) showed the highest similarity (100%) with S. acidiscabies type strain 84-01-182 (GenBank Accession No. FJ007427.1). Pathogenicity of the isolate was tested on tuber slices. The isolate was grown on YME for 5 to 7 days at 28°C and agar plugs from the sporulating colonies were inverted onto excised tuber tissue. Disks were incubated at 28°C in the dark and the presence of necrosis was evaluated after 5 days (1). All tuber slice assays were repeated three times. The noninoculated control tuber slices did not show any necrosis, while those inoculated with the strain did. To our knowledge, this is the first report of S. acidiscabies causing potato scab disease in Uruguay. References: (1) D. H. Park et al. Plant Dis. 87:1290, 2003. (2) L. A. Wanner. Phytopathology 96:1361, 2006.
Sickle‐cell disease and the heart: review of the current literature
Authors: Voskaridou Ersi , Christoulas Dimitrios , Terpos Evangelos 
Published: April 2012

SummarySickle cell disease (SCD) is an inherited chronic haemolytic anaemia whose clinical manifestations arise from the tendency of the haemoglobin to polymerize and deform red blood cells into the characteristic sickle shape due to a single nucleotide change in the β‐globin. Vascular occlusion of small and large vessels can lead to chronic damage of multiple organs including brain, lung, bone, kidney, liver, spleen, and retina. However, the extent to which SCD impacts myocardial function is not very clear. Cardiovascular manifestations include both right and left ventricular systolic and diastolic dysfunction, elevated cardiac output, cardiomegaly and myocardial ischaemia. Progressive heart damage from iron overload occurs in patients requiring routine transfusion therapy. Pulmonary hypertension resulting from intravascular haemolysis has also been recognized as a major complication that independently correlates with survival. This review summarizes all available data for the heart complications in SCD to update the physicians for their appearance, diagnostic procedures and possible management.
Effect of hypoxia and dexamethasone on inflammation and ion transporter function in pulmonary cells
Authors: Urner M , Herrmann I K , Booy C , Roth-Z' Graggen B , Maggiorini M , Beck-Schimmer B 
Published: April 2012

Summary
Dexamethasone has been found to reduce the incidence of high-altitude pulmonary oedema. Mechanisms explaining this effect still remain unclear. We assessed the effect of dexamethasone using established cell lines, including rat alveolar epithelial cells (AEC), pulmonary artery endothelial cells (RPAEC) and alveolar macrophages (MAC), in an environment of low oxygen, simulating a condition of alveolar hypoxia as found at high altitude. Inflammatory mediators and ion transporter expression were quantified. Based on earlier results, we hypothesized that hypoxic conditions trigger inflammation. AEC, RPAEC and MAC, pre-incubated for 1 h with or without dexamethasone (10−7 mol/l), were subsequently exposed to mild hypoxia (5% O2, or normoxia as control) for 24 h. mRNA and protein levels of cytokine-induced neutrophil chemoattractant-1, monocyte chemoattractant protein-1 and interleukin-6 were analysed. mRNA expression and functional activity of the apical epithelial sodium channel and basolateral Na+/K+-ATPase were determined using radioactive marker ions. In all three types of pulmonary cells hypoxic conditions led to an attenuated secretion of inflammatory mediators, which was even more pronounced in dexamethasone pretreated samples. Function of Na+/K+-ATPase was not significantly influenced by hypoxia or dexamethasone, while activity of epithelial sodium channels was decreased under hypoxic conditions. When pre-incubated with dexamethasone, however, transporter activity was partially maintained. These findings illustrate that long-term hypoxia does not trigger an inflammatory response. The ion transport across apical epithelial sodium channels under hypoxic conditions is ameliorated in cells treated with dexamethasone.
The wheat chloroplastic small heat shock protein (sHSP26) is involved in seed maturation and germination and imparts tolerance to heat stress
Authors: CHAUHAN HARSH , KHURANA NEETIKA , NIJHAVAN AASHIMA , KHURANA JITENDRA P. , KHURANA PARAMJIT 
Published: April 2012

ABSTRACTThe nuclear‐encoded chloroplast small heat shock proteins (sHSPs) are present in all plant species from algae to angiosperms. Expression analysis shows that the wheat chloroplastic sHSP (HSP26) is highly inducible by heat stress in almost all the vegetative and generative tissues and is also expressed constitutively in certain developmental growth stages. We characterize wheat chloroplastic sHSP 26 through transgenic approach using Arabidopsis and report cloning of the promoter and its characterization. Transgenic Arabidopsis plants were substantially tolerant under continuous high temperature regimen than wild‐type plants, as measured by photosystem II (PSII) activity, accumulation of more photosynthetic pigments, higher biomass and seed yield. Transgenic plants produced bold seeds under high temperature, having higher germination potential than the wild‐type plants. Further, antisense Arabidopsis plants showed negligible tolerance even for non‐lethal heat shock, impaired in basal thermo‐tolerance, and accumulated less biomass and seed yield under normal growth conditions. Promoter analysis revealed the presence of several heat and other abiotic stress responsive cis‐acting elements along with developmental stage and tissue‐specific elements. Analysis of promoter through GUS reporter system in both transgenic rice and Arabidopsis further confirms the role of chloroplastic sHsp26 in heat and other abiotic stresses as well as during seed maturation and germination. Genome‐wide expression analysis of overexpression Arabidopsis plants revealed that the transcriptome remained unchanged in the transgenic plants and the tolerance was due to the overexpression of chloroplastic heat shock protein (HSP) only.
Assessment of Groundnut under Combined Heat and Drought Stress
Authors: Hamidou F. , Halilou O. , Vadez V. 
Published: April 2012

AbstractIn semi‐arid regions, particularly in the Sahel, water and high‐temperature stress are serious constraints for groundnut production. Understanding of combined effects of heat and drought on physiological traits, yield and its attributes is of special significance for improving groundnut productivity. Two hundred and sixty‐eight groundnut genotypes were evaluated in four trials under both intermittent drought and fully irrigated conditions, two of the trial being exposed to moderate temperature, while the two other trials were exposed to high temperature. The objectives were to analyse the component of the genetic variance and their interactions with water treatment, year and environment (temperature) for agronomic characteristics, to select genotypes with high pod yield under hot‐ and moderate‐temperature conditions, or both, and to identify traits conferring heat and/or drought tolerance. Strong effects of water treatment (Trt), genotype (G) and genotype‐by‐treatment (GxTrt) interaction were observed for pod yield (Py), haulm yield (Hy) and harvest index (HI). The pod yield decrease caused by drought stress was 72 % at high temperature and 55 % at moderate temperature. Pod yield under well‐watered (WW) conditions did not decrease under high‐temperature conditions. Haulm yield decrease caused by water stress (WS) was 34 % at high temperature and 42 % under moderate temperature. Haulm yield tended to increase under high temperature, especially in one season. A significant year effect and genotype‐by‐environment interaction (GxE) effect were also observed for the three traits under WW and WS treatments. The GGE biplots confirmed these large interactions and indicated that high yielding genotypes under moderate temperature were different to those at high temperature. However, several genotypes with relatively high yield across years and temperature environments could be identified under both WW and WS conditions. Correlation analysis between pod weight and traits measured during plant growth showed that the partition rate, that is, the proportion of dry matter partitioned into pods, was contributing in heat and drought tolerance and could be a reliable selection criterion for groundnut breeding programme. Groundnut sensitivity to high‐temperature stress was in part related to the sensitivity of reproduction.
Socio‐economic and ethnic differences in the prevalence of overweight and obesity among school children
Authors: Achat Helen M , Stubbs Joanne M 
Published: April 2012

Aims:  To trial the collection of measurements to provide population‐based prevalence of overweight and obesity in school children in western Sydney and examine the association between healthy weight and ethnicity and socio‐economic status (SES) in a socio‐economically and culturally diverse population.Methods:  A cross‐sectional population‐based survey of 2341 children in Years 4 and 7 (mean ages 9 and 12 years, respectively) in 2007.Results:  Nineteen per cent of children were overweight and a further 6% were obese. The prevalence of combined overweight and obesity was similar for boys and girls (26% vs. 24%, P= 0.35). SES was significantly associated with the prevalence of unhealthy weight: the odds of being overweight or obese were 1.79 times (95% confidence interval (CI) 1.35 to 2.36) higher for children from the lowest quartile than for children from the highest quartile. Compared to children from an English speaking background, children from a non‐English speaking background were significantly more likely to be overweight or obese (21% vs. 31%, P < 0.001). The prevalence of combined overweight and obesity was significantly higher for children from a Pacific Island (odds ratio (OR) 2.66, 95% CI 1.63 to 4.33), Middle Eastern (OR 1.63, 95% CI 1.22 to 2.17) or European (OR 1.67, 95% CI 1.12 to 2.49) background than for English speaking background children.Conclusion:  Large jumps in the prevalence of overweight and obesity in children observed from the 1980s appear to be diminishing, with comparable prevalence reports in 2004 and 2007. Ethnicity and SES are each independently associated with the prevalence of unhealthy weight in children.