bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased expression of annexin I is associated with drug‐resistance in nasopharyngeal carcinoma and other solid tumors
Authors: Chow Betty H. Y. , Chua Daniel T. T. , Sham Jonathan S. T. , Zhang Min‐Yue , Chow Louis W. C. , Bi Jiong , Ma Ning‐Fang , Xie Dan , Loo Wings T. Y. , Fung Jackie M. W. , Fu Li , Guan Xin‐Yuan 
Published: July 2009

AbstractAdjuvant chemotherapy alongside radiotherapy is one of the effective therapies in nasopharyngeal carcinoma (NPC) treatment. However, the appearance of drug resistance is a major obstacle for anti‐cancer chemotherapy and often causes failure of the chemotherapy. In this study, a drug‐resistant gene annexin I (ANX‐I) was identified by comparing differentially expressed proteins between a cisplatin (CDDP)‐resistant NPC cell line CNE2‐CDDP and parental CNE2 cells using 2‐DE. When ANX‐I was transfected into CNE2 cells, the CDDP resistance of CNE2 cells was dramatically increased. The drug‐resistant ability of ANX‐I was demonstrated by both in vitro and in vivo assays. The association of ANX‐I expression with clinical features was also investigated. Increased expression of ANX‐I was significantly associated with disease relapse in NPC (p<0.05). In breast and gastric cancer, increased expression of ANX‐I was significantly associated with drug resistance (p<0.001) and poor prognosis (p<0.001), respectively. Taken together, our findings suggest that ANX‐I plays an important role in drug resistance.
Proteomic profiling of heat shock proteins: An emerging molecular approach with direct pathophysiological and clinical implications
Authors: Bottoni Patrizia , Giardina Bruno , Scatena Roberto 
Published: July 2009

AbstractThe HSP family is one of the most ancient and evolutionarily conserved protective protein families found in nature. Originally discovered as inducible molecules capable of maintaining cellular homeostasis against abrupt temperature changes, HSPs were later determined to represent an adaptive physiological response that copes with a variety of different cellular proteotoxic stresses. These physiological molecular chaperones facilitate the synthesis, folding, assembly, trafficking and secretion of specific proteins in various cellular compartments. Most importantly, these proteins guard the whole cell proteome against misfolding and inappropriate aggregation. A series of diversified proteotoxic stresses, including heat, hypoxia/ischemia, free radicals, acidosis, ATP depletion and toxins are capable of inducing a typical cellular stress response characterised by rapid inhibition of overall protein synthesis, with a concomitant dramatic increase in HSP expression. From a pathophysiological point of view, HSP induction has been observed in a wide spectrum of inflammatory and degenerative diseases (from cancer to prion disease by passing to infective and autoimmune diseases) and, intriguingly, overexpression monitoring seems to have potential implications in terms of diagnosis, prognosis and, above all, therapy. Proteomics studies, identifying a series of modification of HSP expression patterns in different diseases, are confirming these promising clinical applications.
Targeting sarcomas by proteomic approaches
Authors: Madoz‐Gúrpide Juan 
Published: July 2009

AbstractSarcomas are a group of rare and heterogeneous tumors of mesenchymal origin. From the molecular point of view, they are grouped into two main types: (i) simple karyotypes and specific chromosomal translocations, which originate gene and protein fusions; (ii) unspecific gene alterations and complex karyotypes, with numerous gains and losses. When present, chimeric proteins provide specificity in pathogenesis and sarcoma maintenance, acting either as an aberrant transcription factor or altering RNA processing. The descriptions of numerous targets, direct and indirect, emphasize the pivotal role of the fusion over the intervention of secondary events. Accordingly, the elucidation of the cell of origin becomes critical for discovering the early molecular mechanisms involved in sarcomagenesis, as well as the identification of reliable molecular markers and possible therapeutic targets. This review describes the contribution of proteomics to sarcoma research. It reflects how the elucidation of chimeric protein target networks and differential protein expression studies can play a role in identifying the mechanisms of development and progression of sarcomas, as these proteins seem to be involved in tumor metastasis, apoptosis, and other oncogenic processes. Moreover, it may be beneficial in the diagnostic clinical domain, as it may lead to biology‐based therapeutic strategies for sarcomas.
Purification and characterisation of proteins secreted by the entomopathogenic fungus Metarhizium anisopliae with insecticidal activity against adults of the Mediterranean fruit fly, Ceratitis capitata (Diptera: Tephritidae)
Authors: Ortiz‐Urquiza Almudena , Garrido‐Jurado Inmaculada , Santiago‐Álvarez Cándido , Quesada‐Moraga Enrique 
Published: July 2009

AbstractBACKGROUND: The control of the Mediterranean fruit fly (medfly) Ceratitis capitata (Wied) is usually performed with protein bait sprays incorporating chemical insecticides that may have adverse effects on humans, non‐target organisms and the environment. In recent years, scientists have sought more environmentally friendly insecticides for medfly control, such as plant‐ and microorganism‐derived compounds. Among these compounds, entomopathogenic fungi are an unexplored source of natural insecticides.RESULTS: The crude soluble protein extract (CSPE) of the entomopathogenic fungus Metarhizium anisopliae (Mestch.) (strain EAMa 01/58‐Su) shows chronic insecticidal activity when administered per os. Mortality in flies exhibits a dose response. The CSPE produces an antifeedant effect in adult flies, a result probably due to a progressive deterioration of the fly midgut after ingestion of the extract. Protease and temperature treatments show that insecticidal activity against C. capitata is due to proteinaceous compounds that are highly thermostable. Four monomeric proteins from this crude extract have been purified by liquid chromatography and gel electroelution. Although all four monomers seem to be involved in the insecticidal activity of the CSPE, the 15 kDa and the 11 kDa proteins appear to be mainly responsible for the observed insecticidal effect.CONCLUSIONS: Four new fungal proteins with insecticidal activity have been purified and identified. These proteins might be combined with insect baits for C. capitata biocontrol. Copyright © 2009 Society of Chemical Industry
An engineered tryptophan zipper‐type peptide as a molecular recognition scaffold
Authors: Cheng Zihao , Campbell Robert E. 
Published: July 2009

AbstractIn an effort to develop a structured peptide scaffold that lacks a disulfide bond and is thus suitable for molecular recognition applications in the reducing environment of the cytosol, we investigated engineered versions of the trpzip class of β‐hairpin peptides. We have previously shown that even most highly folded members of the trpzip class (i.e. the 16mer peptide HP5W4) are substantially destabilized by the introduction of mutations in the turn region and therefore not an ideal peptide scaffold. To address this issue, we used a FRET‐based live cell screening system to identify extended trpzip‐type peptides with additional stabilizing interactions. One of the most promising of these extended trpzip‐type variants is the 24mer xxtz1‐peptide with the sequence KAWTHDWTWNPATGKWTWLWRKNK. A phage display library of this peptide with randomization of six residues with side chains directed towards one face of the hairpin was constructed and panned against immobilized streptavidin. We have also explored the use of xxtz1‐peptide for the presentation of an unstructured peptide ‘loop’ inserted into the turn region. Although NMR analysis provided no direct evidence for structure in the xxtz1‐peptide with the loop insertion, we did attempt to use this construct as a scaffold for phage display of randomized peptide libraries. Panning of the resulting libraries against streptavidin resulted in the identification of peptide sequences with submicromolar affinities. Interestingly, substitution of key residues in the hairpin‐derived portion of the peptide resulted in a 400‐fold decrease in Kd, suggesting that the hairpin‐derived portion plays an important role in preorganization of the loop region for molecular recognition. Copyright © 2009 European Peptide Society and John Wiley & Sons, Ltd.
The Fermi surface of a superconductor: OsB2
Authors: Hebbache M. 
Published: July 2009

AbstractOsmium diboride has been known for some time as a low compressibility material and a superhard material. It is suitable for hard coating applications. It is also a superconductor below 2.1 K. Using first‐principles calculations, the author investigated the geometry of its Fermi surface (FS) and calculated the related physical quantities. The theoretical results are used to predict the frequencies of the Shubnikov–de Haas quantum oscillations. Comparison with recent measurements of the magneto‐resistance oscillations in osmium diboride is made.magnified imageThe picture shows the FS of OsB2 consisting of three sheets: a pair of two nested ellipsoidal surfaces and a corrugated tubular surface.(© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Ab initio predictions of stability and electronic properties of cubic rhodium carbides RhCx as dependent on carbon content
Authors: Bannikov V. V. , Shein I. R. , Ivanovskii A. L. 
Published: July 2009

AbstractThe trends in stability, structural and electronic properties of cubic rhodium carbides as dependent on carbon stoichiometry RhCx (1 ≤ x ≤ 0.25) were studied by means of the first principles FLAPW‐GGA calculations. As distinct from carbon‐rich phases RhC1.00–0.33 with positive formation energies (Eform > 0), the carbon‐deficient sub ‐carbide RhCx ≤0.25 was found to be stable (Eform < 0), and therefore it may be synthesized. Moreover, an unusual effect of “metallization” of fcc Rh owing to the introduction of carbon was predicted. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Downregulation of pro‐inflammatory cytokines by lupeol measured using cytometric bead array immunoassay
Authors: Ahmad Sheikh Fayaz , Pandey Anjali , Kour Kiranjeet , Bani Sarang 
Published: July 2009

AbstractThe objective of the study was to investigate the activity of Lupeol (LUP) on proinflammatory and anti‐inflammatory cytokines in the pleural exudate from male swiss albino mice. We applied Cytometric bead array technology for simultaneously measurement of these cytokines in pleurisy induced mice treated with lupeol in graded oral doses. Cytometric bead array uses the sensitivity of amplified fluorescence detection by flowcytometer to measure soluble analytes in a particle based immune assay. This assay can accurately quantitate 5 cytokines in a 50 microlitre sample volume. Oral administration of LUP at doses of 25, 50, 100 and 200 mg/kg p.o. produced dose related inhibition of IL‐2, IFN‐gamma and TNF‐α in the pleural exudate with the most significant effect at 100 mg/kg oral dose. LUP had a non significant inhibitory effect on the levels of IL‐4 and IL‐5. Copyright © 2009 John Wiley & Sons, Ltd.
Oligonol, a new lychee fruit‐derived low‐molecular form of polyphenol, enhances lipolysis in primary rat adipocytes through activation of the ERK1/2 pathway
Authors: Ogasawara Junetsu , Kitadate Kentaro , Nishioka Hiroshi , Fujii Hajime , Sakurai Takuya , Kizaki Takako , Izawa Tetsuya , Ishida Hitoshi , Ohno Hideki 
Published: July 2009

AbstractThe effect of Oligonol, a phenolic product from lychee fruit polyphenol (LFP) containing catechin‐type monomers and lower oligomers of proanthocyanidin, on lipolysis in primary adipocytes was investigated in order to examine the possible mechanism underlying the regulation of in vivo metabolism in fat. Oligonol significantly increased lipolysis, which was accompanied by both activation of extracellular signaling‐related kinase 1/2 (ERK1/2) and down‐regulation of perilipin protein expression, without an increase in intracellular cAMP production. The increase in lipolysis with Oligonol was prevented completely by pretreatment with either PD98059 or U0126, selective ERK1/2 inhibitors, which also prevented the reduction in the expression of perilipin protein. Tumor necrosis factor‐α also down‐regulated the expression of perilipin protein. However, there was no significant alteration in the expression of Gαi protein with Oligonol. These findings indicate that Oligonol enhances lipolysis in primary adipocytes, independent of cAMP production, but its effect is dependent on activation of the ERK1/2 pathway, leading to down‐regulation of perilipin protein expression. Copyright © 2009 John Wiley & Sons, Ltd.
Anticholinesterase activity of 7‐methoxyflavones isolated from Kaempferia parviflora
Authors: Sawasdee Pattara , Sabphon Chalisa , Sitthiwongwanit Duangporn , Kokpol Udom 
Published: July 2009

AbstractThe rhizome of Kaempferia parviflora or kra‐chai‐dum (in Thai) is used traditionally as a folk medicine. The preliminary cholinesterase inhibitory screening of this plant extract exhibited significant acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitory activities. Thirteen known methoxyflavones (1–13) were isolated and their structures were completely elucidated based on NMR analysis and compared with literature reports. Minor compounds 12–13 were reported for the first time from this species. The cholinesterase inhibitory test results showed that the highest potential inhibitors toward AChE and BChE were 5,7,4′‐trimethoxyflavone (6) and 5,7‐dimethoxyflavone (7), respectively, with the percentage inhibitory activity varying over 43–85%. The structure‐activity relationship study led to the conclusion that compounds bearing 5,7‐dimethoxy groups and a free substituent at C‐3 had a significant inhibitory effect at a concentration of 0.1 mg/mL, but those bearing a 5‐hydroxyl group reduced the inhibitory potency. On the other hand, flavones bearing a 3′‐ or 5′‐methoxy group did not influence the inhibitory effect. Interestingly, 5,7‐dimethoxyflavone (7) exhibited strong selectivity for BChE over AChE which may be of great interest to modify as a treatment agent for Alzheimer's disease. Copyright © 2009 John Wiley & Sons, Ltd.
The beta‐carboline alkaloid harmine inhibits BCRP and can reverse resistance to the anticancer drugs mitoxantrone and camptothecin in breast cancer cells
Authors: Ma Yonggang , Wink Michael 
Published: July 2009

AbstractMultidrug resistance (MDR), mediated by highly expressed ABC transporters, is one of the most important mechanisms in tumor cells. Breast cancer resistance protein (BCRP) is a member of the ABC transporter family. This transporter expels different kinds of lipophilic anticancer drugs, which have diffused into the cells. In this study, 96‐well plate based assays and flow cytometry analysis were employed to screen natural products for BCRP inhibition. The beta‐carboline alkaloid harmine inhibited BCRP in a BCRP overexpressing breast cancer cell line (MDA‐MB‐231). Harmine reduced resistance to the anticancer drugs mitoxantrone and camptothecin mediated by BCRP and might be an interesting new reversal agent. Harmine did not inhibit P‐glycoprotein (P‐gp) mediated drug efflux. Copyright © 2009 John Wiley & Sons, Ltd.
(‐)‐epigallocatechin‐3‐gallate reduces experimental colon injury in rats by regulating macrophage and mast cell
Authors: Mochizuki Miyako , Hasegawa Noboru 
Published: July 2009

AbstractThe ameliorative effect of (‐)‐epigallocatechin‐3‐gallate (EGCG) on inflammatory bowel disease (IBD) induced by ethanol 2,4,6‐trinitrobenzene sulfonic acid (TNBS) was studied in 7‐week‐old male rats. Intestinal lesions were measured as an increase in myeloperoxidase (MPO) activity in mucosa. The supplementation of EGCG significantly inhibited MPO activity and histamine levels in the distal colon mucosa. The EGCG inhibited macrophage chemotaxis toward N‐formyl‐l‐methionyl‐l‐leucyl‐l‐phenylalanine in a concentration‐dependent manner. These observations confirmed that EGCG can ameliorate acute experimental colitis by the suppression of mast cells and macrophage activities. Copyright © 2009 John Wiley & Sons, Ltd.
Validating circuit board Interconnect Stress Test preconditioning processes using statistical model comparisons of accelerated test data
Authors: Juarez Joseph M. , White Vicka 
Published: July 2009

AbstractInterconnect stress testing (IST) is used for acceptance of product by testing coupons fabricated along with the production panels to determine the long‐term reliability of the plated thru‐holes. Prior to in‐service use, the actual production circuit boards are subjected to Circuit Card Assembly processes like soldering components, but simulated on coupons using preconditioning cycles (PCCs). In this paper, the Circuit Card Assembly preconditioning process is compared with IST PCCs. Accelerated test data for this study use IST preconditioning and Re‐Flow Oven preconditioning at an independent company laboratory from coupons supplied by three fabrication suppliers. The data indicate that a Weibull distribution be applied for statistical model comparisons using the likelihood‐ratio test on both types of preconditioning processes with coupons that are then subjected to subsequent in‐service use IST cycles until failure occurs. Results show that using six IST PCCs are statistically equivalent to six Re‐Flow Oven PCCs and thus five IST PCCs currently in use should be reconsidered, but the advantages of effective IST preconditioning lower acceptance costs significantly. In addition, the statistical results are validated by root mean square power calculations. Copyright © 2009 John Wiley & Sons, Ltd.
Analysis of computer experiments with multiple noise sources
Authors: Dehlendorff Christian , Kulahci Murat , Andersen Klaus K. 
Published: July 2009

AbstractIn this paper we present a modeling framework for analyzing computer models with two types of variations. The paper is based on a case study of an orthopedic surgical unit, which has both controllable and uncontrollable factors. Our results show that this structure of variation can be modeled effectively with linear mixed effects models and generalized additive models. Copyright © 2009 John Wiley & Sons, Ltd.
Docking and Virtual Screening Using Distributed Grid Technology
Authors: García‐Sosa Alfonso T. , Sild Sulev , Maran Uko 
Published: July 2009

AbstractDistributed grid technologies are gradually realizing their potential to provide innovative infrastructures for complex scientific and industrial applications in the field of computational chemistry and related application areas. The current paper gives examples of distributed solutions for docking and virtual screening applications in moving the computational paradigms towards collaborative research and grid computing environments. The Chemomentum collaborative computing environment including both hardware and software infrastructure is described. Examples of applications are given i) for docking and virtual screening on multiprotein and multilibrary cases for H5N1 avian influenza and HIV‐1 viruses; and ii) QSAR model building related to HIV‐1 protease activity and aquatic toxicity.
COSMO‐RS as a Predictive Tool for Lipophilicity
Authors: Wittekindt Carsten , Klamt Andreas 
Published: July 2009

AbstractThe COSMO‐RS method was applied to predict altogether 4155 experimental available values of 12 different hydrocarbon‐water (logPalk) and 1‐n‐octanol‐water (logPow) partition coefficients. The over all results of the correlation between the predicted and experimental partition coefficients demonstrate that COSMO‐RS is able to a priori predict the values of all systems under investigation for almost all organic compounds. As a showcase, the outliers of the hexadecane‐water system were critically assessed. In two cases the prediction could be improved by correcting the underlying chemical structure. The remaining three outliers are due to known problems of seldom elements (germanium compounds) or of insufficient conformational representation of the molecule (crown‐ethers). Finally, ΔlogP which is the difference between (logPow−logPalk) is correlated with the blood‐brain partition coefficient (logBB). On an external test set of 199 compounds the logBB prediction based on ΔlogP is slightly inferior to our existing QSPR model (RMSE: 0.52 vs. 0.46)
Exact computation and asymptotic approximations of 6j symbols: Illustration of their semiclassical limits
Authors: Ragni Mirco , Bitencourt Ana Carla Peixoto , Da S. Ferreira Cristiane , Aquilanti Vincenzo , Anderson Roger W. , Littlejohn Robert G. 
Published: July 2009

AbstractThis article describes a direct method for the exact computation of 3nj symbols from the defining series, and continues discussing properties and asymptotic formulas focusing on the most important case, the 6j symbols or Racah coefficients. Relationships with families of hypergeometric orthogonal polynomials are presented and the asymptotic behavior is studied to account for some of the most relevant features, both from the viewpoints of the basic geometrical significance and as a source of accurate approximation formulas, such as those due to Ponzano and Regge and Schulten and Gordon. Numerical aspects are specifically investigated in detail, regarding the relationship between functions of discrete and of continuous variables, exhibiting the transition in the limit of large angular momenta toward both Wigner's reduced rotation matrices (or Jacobi polynomials) and harmonic oscillators (or Hermite polynomials). © 2009 Wiley Periodicals, Inc. Int J Quantum Chem, 2010
Is Cu involved in prion oligopeptide stability? Experiments and numerical simulations
Authors: Minicozzi V. , Morante S. 
Published: July 2009

AbstractThe high‐sociological impact of neurodegenerative diseases (like Alzheimer disease, Transmissible Spongiform Encephalopathies, Parkinson disease, etc.) has renewed the interest of researchers in the study of misfolding processes and in particular of the rôle played by metals in plaque formation as their unbalanced concentration can be regarded as a possible concurrent cause of protein aggregation. Metals are essential players in many of the fundamental activities of cells. Storing, metabolism, and trafficking of metals through the cellular membrane and within the cytoplasm are mediated by many proteins via well‐tuned mechanisms because of the toxicity of free ions. In this review article, we summarize the results of the most recent experimental and numerical investigations aimed at understanding the possible rôle of Cu in stabilizing the Prion protein structure and in the formation of protein polymers. © 2009 Wiley Periodicals, Inc. Int J Quantum Chem, 2010
Optimized perturbation theory scheme for calculating the interatomic potentials and hyperfine lines shift for heavy atoms in the buffer inert gas
Authors: Malinovskaya S. V. , Glushkov A. V. , Khetselius O. Yu. , Svinarenko A. A. , Mischenko E. V. , Florko T. A. 
Published: July 2009

AbstractA new relativistic approach, based on the gauge‐invariant perturbation theory (PT), is applied to calculate the interatomic potentials and hyperfine structure line collision shifts for the alkali and lanthanide atoms in an atmosphere of the inert gases. Data on the interatomic potentials and collision shifts for Rb, Cs, and Yb atoms in an atmosphere of the inert gas He are presented. © 2009 Wiley Periodicals, Inc. Int J Quantum Chem, 2009