bluuebunny/crossref_metadata_2025_split huggingface dataset:
Robust stability of Markovian jumping genetic regulatory networks with disturbance attenuation
Authors: Yao Yingtao , Liang Jinling , Cao Jinde 
Published: March 2011

AbstractBecause of intracellular and extracellular noise perturbations and environment fluctuations, gene regulation is an intrinsically noisy process. In this paper, we present a hybrid genetic regulatory network (GRN) model which is based on the Markov chain. The GRNs are composed of N modes and the network switches from one mode to another according to a Markov chain with known transition probability. Time‐delays here are mode‐dependent. Based on the Lyapunov stability theory and the linear matrix inequality (LMI) technique, sufficient conditions are given to ensure the stochastic stability of the GRNs with polytopic uncertainties and disturbance attenuation. All the conditions are presented in terms of LMIs which are easily verified via the LMI toolbox. Examples are provided to illustrate the effectiveness of the theoretical results.Copyright © 2011 John Wiley and Sons Asia Pte Ltd and Chinese Automatic Control Society
Effect of alcohol drinking and cigarette smoking on neutrophil functions in adults
Authors: Sato Junya , Takahashi Ippei , Umeda Takashi , Matsuzaka Masashi , Danjyo Kazuma , Tsuya Ryosuke , Kida Kazuyuki , Takami Hideki , Nakaji Shigeyuki 
Published: March 2011

ABSTRACTIn recent years, the effects of smoking and excessive alcohol consumption on immune function have been studied, due to a high prevalence of infection or cancer in heavy drinkers, and the combination of smoking and drinking was considered to be a carcinogenic risk. However, the effect of smoking and drinking on systemic immune function has yet to be clearly understood. In this study, we investigated neutrophil functions (reactive oxygen species (ROS) productive activity, phagocytic ability and serum opsonic activity) and their relationship with alcohol consumption or amount of smoking. In total there were 731 male and female adult subjects who participated in the Iwaki Health Promotion Project in 2005. Multiple regression analysis showed a trend of increased ROS production in male subjects and a statistically significant decrease was observed in phagocytic activity caused by smoking in female subjects. In other words, oxidative stress caused by smoking in male subjects may be involved in ROS production from neutrophils. Decreased phagocytic activity of neutrophils caused by smoking suggests that host defense functions were impaired in female subjects. A relationship between neutrophil functions and the amount of alcohol consumption was not observed. Copyright © 2011 John Wiley & Sons, Ltd.
Mapping the yeast host cell response to recombinant membrane protein production: Relieving the biological bottlenecks
Authors: Ashe Mark P. , Bill Roslyn M. 
Published: March 2011

AbstractUnderstanding the structures and functions of membrane proteins is an active area of research within bioscience. Membrane proteins are key players in essential cellular processes such as the uptake of nutrients, the export of waste products, and the way in which cells communicate with their environment. It is therefore not surprising that membrane proteins are targeted by over half of all prescription drugs. Since most membrane proteins are not abundant in their native membranes, it is necessary to produce them in recombinant host cells to enable further structural and functional studies. Unfortunately, achieving the required yields of functional recombinant membrane proteins is still a bottleneck in contemporary bioscience. This has highlighted the need for defined and rational optimization strategies based upon experimental observation rather than relying on trial and error. We have published a transcriptome and subsequent genetic analysis that has identified genes implicated in high‐yielding yeast cells. These results have highlighted a role for alterations to a cell's protein synthetic capacity in the production of high yields of recombinant membrane protein: paradoxically, reduced protein synthesis favors higher yields. These results highlight a potential bottleneck at the protein folding or translocation stage of protein production.
An empirical modeling platform to evaluate the relative control discrete CHO cell synthetic processes exert over recombinant monoclonal antibody production process titer
Authors: McLeod Jane , O'Callaghan Peter M. , Pybus Leon P. , Wilkinson Stephen J. , Root Tracy , Racher Andrew J. , James David C. 
Published: March 2011

AbstractIn this study we have combined empirically derived mathematical models of intracellular Mab synthesis to quantitatively compare the degree to which individual cellular processes limit recombinant IgG4 monoclonal antibody production by GS‐CHO cells throughout a state‐of‐the‐art industrial fed‐batch culture process. Based on the calculation of a production process control coefficient for each stage of the intracellular Mab synthesis and secretion pathway, we identified the major cellular restrictions on Mab production throughout the entire culture process to be recombinant heavy chain gene transcription and heavy chain mRNA translation. Surprisingly, despite a substantial decline in the rate of cellular biomass synthesis during culture, with a concomitant decline in the calculated rate constants for energy‐intensive Mab synthetic processes (Mab folding/assembly and secretion), these did not exert significant control of Mab synthesis at any stage of production. Instead, cell‐specific Mab production was maintained by increased Mab gene transcription which offset the decline in cellular biosynthetic rates. Importantly, this study shows that application of this whole‐process predictive modeling strategy should rationally precede and inform cell engineering approaches to increase production of a recombinant protein by a mammalian host cell—where control of productivity is inherently protein product and cell line specific. Biotechnol. Bioeng. 2011;108:2193–2204. © 2011 Wiley Periodicals, Inc.
Real‐time PCR (qPCR) primer design using free online software
Authors: Thornton Brenda , Basu Chhandak 
Published: March 2011

AbstractReal‐time PCR (quantitative PCR or qPCR) has become the preferred method for validating results obtained from assays which measure gene expression profiles. The process uses reverse transcription polymerase chain reaction (RT‐PCR), coupled with fluorescent chemistry, to measure variations in transcriptome levels between samples. The four most commonly used fluorescent chemistries are SYBR® Green dyes and TaqMan®, Molecular Beacon or Scorpion probes. SYBR® Green is very simple to use and cost efficient. As SYBR® Green dye binds to any double‐stranded DNA product, its success depends greatly on proper primer design. Many types of online primer design software are available, which can be used free of charge to design desirable SYBR® Green‐based qPCR primers. This laboratory exercise is intended for those who have a fundamental background in PCR. It addresses the basic fluorescent chemistries of real‐time PCR, the basic rules and pitfalls of primer design, and provides a step‐by‐step protocol for designing SYBR® Green‐based primers with free, online software. Biochemistry and Molecular Biology Education Vol. 39, No. 2, pp. 145–154, 2011
Intergenerational possession transfers and identity maintenance
Authors: Curasi Carolyn F. 
Published: March 2011

AbstractResearch suggests that older consumers hope to transfer their cherished possessions, bundled with their carefully edited personal history, to younger family members. Further, researchers have found that older consumers worry that their younger loved ones will not care about these cherished, transferred possessions. Using a random, national US sample, this research investigates these findings, examining the pervasiveness of some of the most common themes emanating from previous naturalistic investigations, thus broadening our understanding of intergenerational possession transfers.Findings indicate that many of the key themes in intergenerational possession transfers conducted in naturalistic investigations can be generalized to a majority of the adult population in the US. That is, in this random, national US sample, a majority of respondents report: that they hope their loved ones will care about their cherished possessions, that they plan to pass their family possessions on to subsequent generations, and that their familial possessions reflect their family identity. Findings also indicate that this was significant among all adult age groups, and that females value cherished possessions more than males. Copyright © 2011 John Wiley & Sons, Ltd.
Optimized Production of Protein Crystals: From 1D Crystallization Slot towards 2D Supersaturation B22 Diagram
Authors: Wanka J. , Peukert W. 
Published: March 2011

AbstractThe second osmotic virial coefficient, B22, of protein solutions is a function of the protein interaction potentials and, thus, summarizes many solution parameters leading to crystallization or precipitation of proteins. B22 of hen egg‐white lysozyme and bovine serum albumin was measured by static light scattering under the variation of pH, temperature, and crystallization agents. Comparing B22 values and precipitate morphology in a systematically analyzed range of solution conditions allows the construction of the so‐called crystallization slot. The thermodynamic driving force for crystallization is supersaturation, which is set by the protein concentration and the solubility (related to B22). Hence, besides the optimal B22 range, the protein concentration and, if solubilities are available, the supersaturation leads to a two‐dimensional crystallization window where crystal formation is possible. This is shown in a generalized state diagram, where protein morphologies are solely determined as a function of protein concentration or supersaturation and interactions.
Growth Kinetics of Cane Sugar Crystals by Image Analysis
Authors: Parisi M. , Ribeaux Kindelán G. , Chianese A. 
Published: March 2011

AbstractThis work reports an experimental investigation on sucrose crystal growth. Single crystals in a supersaturated solution were observed under a digital microscope. The changes in crystal lengths along the two dimensions with time were measured and the linear growth rates were determined. Significant growth rate dispersion among different crystals was detected so that further investigation of crystal growth was performed on the same crystal. The effects of temperature and mother liquor concentration on crystal growth were investigated and the obtained results were in agreement with other research works. Finally, the influence of a magnetic field on the growth rate was checked. At an intensity of 0.5 T, the magnetic field seems to slightly enhance the crystal growth rate; however, this effect is quite small.
Effect of Supersaturation and Thermodynamics on Co‐Crystal Formation
Authors: Lee K.‐C. , Kim K.‐J. 
Published: March 2011

AbstractTernary solid‐liquid equilibrium diagrams of the system methanol/carbamazepine (CBZ)/nicotinamide (NCT) were generated and are discussed in the range between 5 and 50 °C. A study of the phase diagrams reveals that co‐crystals could successfully be prepared. An evaporative crystallization method was used to prepare the co‐crystals. By increasing the temperature, the co‐crystal solubilities increased. In most cases, the co‐crystals formed near room temperature. Regions for co‐crystal, CBZ + co‐crystal, NCT + co‐crystal, CBZ crystal, and NCT crystal formation, and for the saturated solution are visible in the ternary diagram. In the region of supersaturation of S > 2.2, co‐crystals were formed.
Influence of Crystallization Conditions on the Growth of Polycrystalline Particles
Authors: Beck R. , Flaten E. , Andreassen J.‐P. 
Published: March 2011

AbstractThe effect of initial supersaturation and temperature on crystal morphology below and above the threshold for the onset of spherulitic growth has been compared for calcium carbonate, L‐glutamic acid, and an aromatic amine derivative in aqueous systems. For the vaterite polymorph of calcium carbonate is has also been documented how spherulites (polycrystalline particles) develop with time in a solvent mixture of water and monoethylene glycol. For the three studied substances, the temperature was found to have different effects on the compactness of the crystals. The influence of supersaturation on the onset of spherulitic growth and particle morphology, however, bears striking similarities for the investigated substances. While monocrystalline particles are found at low supersaturation, polycrystalline particles form with increasing degree of branching when supersaturation is increased.
Classification of Organic Solvents Revisited by Using the COSMO‐RS Approach
Authors: Durand Morgan , Molinier Valérie , Kunz Werner , Aubry Jean‐Marie 
Published: March 2011

AbstractA new approach for the classification of solvents is proposed, based solely on the solvent molecular structure and relying on the “COnductor‐like Screening MOdel for Real Solvents” (COSMO‐RS), in which solvents are considered in their liquid state. This approach provides an a priori classification without requiring the knowledge of any experimental data. The theoretical descriptors of the proposed classification are generated through the analysis of the COSMO‐RS σ‐potential profiles. By applying a two‐step statistical procedure to the descriptors generated for a set of 153 representative solvents, a clustering into 10 classes leads to an optimal classification that is compared to the classical Chastrette’s classification. The investigation of nitrocellulose solubility allows a validation of the proposed classification, which can be extended also to solvent mixtures. This new approach for solvent classification, thus, appears as a consistent tool for the design of new solvents and their formulation.
Fluorometric Sensing of Biogenic Amines with Aggregation‐Induced Emission‐Active Tetraphenylethenes
Authors: Nakamura Mitsutaka , Sanji Takanobu , Tanaka Masato 
Published: March 2011

AbstractA fluorometric sensor for detection and identification of biogenic amines with carboxylic acid modified tetraphenylethenes (TPEs) based on aggregation‐induced emission (AIE) is reported. A mixture of the carboxylic acid substituted TPE and biogenic amines displayed a blue emission on aggregation, which serves as a “turn‐on” fluorescent sensor for the amines, the degree of fluorescence enhancement being dependent on the amine. The chromic responses were utilized to distinguish the amines. A fluorometric sensor array of three TPEs with carboxylic acid groups was shown to identify accurately 10 different amines, including biogenic amines. The response patterns were systematically classified by using linear discriminant analysis (LDA) with 98 % classification accuracy. Additional information on the concentration of histamine in a “tuna fish matrix” as an example was assessed by the further analysis of the fluorescence intensity, demonstrating a test for food freshness and quality.
ChemInform Abstract: Crystal Structure and Raman Spectroscopic Study of K5[CuO2][CO3].
Authors: Ali Naveed Zafar , Nuss Juergen , Jansen Martin 
Published: March 2011

AbstractDark yellow single crystals of the title compound are prepared by heating stoichiometric mixtures of CuO, KN3, KNO3, and K2CO3 followed by annealing at 833 K for 50 h.
ChemInform Abstract: Low‐Energy Path to Dense HfO2 Thin Films with Aqueous Precursor.
Authors: Jiang Kai , Anderson Jeremy T. , Hoshino Ken , Li Dong , Wager John F. , Keszler Douglas A. 
Published: March 2011

AbstractHigh‐quality, smooth and dense HfO2 thin films are deposited by spin coating using an aqueous H2O2/HNO3 solution of the precipitate obtained from an aq.
ChemInform Abstract: Structural Diversity and Thermochromic Properties of Iodobismuthate Materials Containing d‐Metal Coordination Cations: Observation of a High Symmetry [Bi3I11]2‐ Anion and of Isolated I‐ Anions.
Authors: Goforth Andrea M. , Tershansy Meredith A. , Smith Mark D. , Peterson LeRoy Jr. , Kelley Jennifer G. , DeBenedetti William J. I. , zur Loye Hans‐Conrad 
Published: March 2011

AbstractThe new compounds (III), (V), (VI), and (VII) are structurally characterized by single crystal XRD.
ChemInform Abstract: Diastereoselective Michael Addition of Magnesium Amide to O‐(2‐Alkenoyl)TEMPOs and Comparison of Reactivity with Acyl Substituent‐Modified Carboxylic Analogues.
Authors: Ma Li‐Jian , Mei Zhen‐Wu , Toyohara Keisuke , Kawafuchi Hiroyuki , Nokami Junzo , Inokuchi Tsutomu 
Published: March 2011

AbstractHighly diastereoselective addition of Mg amides, derived from optically active secondary amines [cf.
ChemInform Abstract: Quinidine Thiourea‐Catalyzed Aldol Reaction of Unactivated Ketones: Highly Enantioselective Synthesis of 3‐Alkyl‐3‐hydroxyindolin‐2‐ones.
Authors: Guo Qunsheng , Bhanushali Mayur , Zhao Cong‐Gui 
Published: March 2011

AbstractThe asymmetric aldol reaction of unactivated ketones and activated carbonyl compounds is realized with a quinidine‐derived thiourea catalyst, and involves an enolate mechanism instead of the widely used enamine mechanism.
ChemInform Abstract: Alkyl 4‐Chlorobenzoyloxycarbamates as Highly Effective Nitrogen Source Reagents for the Base‐Free, Intermolecular Aminohydroxylation Reaction.
Authors: Harris Lawrence , Mee Simon P. H. , Furneaux Richard H. , Gainsford Graeme J. , Luxenburger Andreas 
Published: March 2011

AbstractIn the presence of OsO4, various alkenes undergo efficient aminohydroxylation with the carbamates (II).
ChemInform Abstract: Reversal of Diastereoselectivity in Reactions of the Trifluoroacetaldehyde Ethyl Hemiacetal with Enamines and Imines: Metal‐Free, Complementary anti‐ and syn‐Selective Synthesis of 4,4,4‐Trifluoro‐1‐aryl‐3‐hydroxy‐2‐methyl‐1‐butanones.
Authors: Funabiki Kazumasa , Matsunaga Kei , Gonda Hiroshi , Yamamoto Hitoshi , Arima Takao , Kubota Yasuhiro , Matsui Masaki 
Published: March 2011

AbstractTreatment of the hemiacetal (II) with imines of type (I) is found to result in efficient formation of syn‐adducts like (III).
ChemInform Abstract: A Practical Iodination of Aromatic Compounds by Using Iodine and Iodic Acid.
Authors: Shinde Avinash T. , Zangade Sainath B. , Chavan Shivaji B. , Vibhute Archana Y. , Nalwar Yogesh S. , Vibhute Yeshwant B. 
Published: March 2011

AbstractA simple, mild, and efficient method for the iodination of various aromatic amines, hydroxy aromatic aldehydes, hydroxy acetophenones and phenols is presented (34 examples).
ChemInform Abstract: Mild Approach to the Deprotection of Troc from Protected Amines Using Mischmetal and TMSCl.
Authors: Vellemaee Eerold , Stepanov Vladimir , Maeeorg Uno 
Published: March 2011

AbstractA new mild and economic deprotection method for 2,2,2‐trichloroethoxycarbonyl‐protected amines in the presence of Tms‐Cl‐activated mischmetal is presented.
ChemInform Abstract: Synthesis of Tri‐ and Diaryloxybenzenes by Rhodium‐Catalyzed Complete Intermolecular [2 + 2 + 2] Cycloaddition of Aryl Ethynyl Ethers.
Authors: Komine Yoshiyuki , Miyauchi Yuta , Kobayashi Masayuki , Tanaka Ken 
Published: March 2011

AbstractA cationic Rh(I)‐H8‐BINAP complex efficiently catalyzes the cross [2 + 2 + 2] cycloaddition of aryl ethynyl ethers (I) with internal alkynes (II) and homo [2 + 2 + 2] cycloaddition of (I).
ChemInform Abstract: The Solar‐Chemical Photo‐Friedel—Crafts Heteroacylation of 1,4‐Quinones.
Authors: Benites Julio , Rios David , Diaz Pilar , Valderrama Jaime A. 
Published: March 2011

AbstractA simple procedure using sunlight irradiation (30 h within 6 days) is presented for the acylation of 1,4‐quinones with heteroaromatic aldehydes.
ChemInform Abstract: Efficient Synthesis of Aryl Methyl Sulfide Derivatives Using (Methylthio)trimethylsilane as Methylthiolation Reagent.
Authors: Qiao Qi , Dominique Romyr , Sidduri Achyutharao , Lou Jianping , Goodnow Robert A. Jr. 
Published: March 2011

AbstractMethyl trimethylsilyl sulfide is used for the first time as methylthiolation reagent for the nucleophilic displacement of aromatic nitro groups.
ChemInform Abstract: Synthesis of Carbonates Directly from 1 atm CO2 and Alcohols Using CH2Cl2.
Authors: Yamazaki Yusuke , Kakuma Kasumi , Du Ya , Saito Susumu 
Published: March 2011

AbstractA new general synthesis of carbonates involves reaction of aliphatic, allylic and benzylic alcohols with carbon dioxide in the presence of Cs2CO3 and CH2Cl2 as reagents.
ChemInform Abstract: P‐Phenyl‐2,2,6,6‐tetramethylphosphorinan‐4‐ol: An Air‐Stable P,O‐Type Ligand for Palladium‐Mediated Cross‐Coupling Reactions.
Authors: Ullah Ehsan , McNulty James , Larichev Vladimir , Robertson Al J. 
Published: March 2011

AbstractThe novel catalyst is applied to the reaction of aryl halides with boronic acids or amines.
ChemInform Abstract: Biopolymer‐Metal Complex Wool—Pd as a Highly Active Heterogeneous Catalyst for Heck Reaction in Aqueous Media.
Authors: Wu Shang , Ma Hengchang , Jia Xiaojie , Zhong Yunmei , Lei Ziqiang 
Published: March 2011

AbstractThe new heterogeneous system Pd—wool is used for reactions of aryl halides with various olefins.
ChemInform Abstract: Rhenium‐Catalyzed Insertion of Terminal Alkenes into a C(sp2)—H Bond and Successive Transfer Hydrogenation.
Authors: Kuninobu Yoichiro , Nakahara Takahiro , Yu Peng , Takai Kazuhiko 
Published: March 2011

AbstractIn the presence of HRe(CO)4 aromatic aldimines react with terminal alkenes to afford synthetically important 2‐alkenylbenzylamines.
ChemInform Abstract: AlCl3‐Promoted Highly Regio‐ and Diastereoselective [3 + 2] Cycloadditions of Activated Cyclopropanes and Aromatic Aldehydes: Construction of 2,5‐Diaryl‐3,3,4‐trisubstituted Tetrahydrofurans.
Authors: Yang Gaosheng , Shen Yue , Li Kui , Sun Yongxian , Hua Yuanyuan 
Published: March 2011

AbstractThe reaction with electron‐rich aromatic aldehydes allows facile and selective access to 2,4‐trans‐2,5‐trans‐pentasubstituted tetrahydrofurans.
ChemInform Abstract: Efficient Synthesis of a Benzo[b]furan Building Block.
Authors: Lee Jaekyoo , Khanapure Subhash P. , Kim Hwa‐Ok , Rajur Raj , Li Bing , Williams John D. , Pai Ramdas , Peet Norton P. 
Published: March 2011

AbstractA practical synthesis of the previously unreported 5‐formylbenzo[b]furan‐2‐carbonitrile (VIII) is described.
ChemInform Abstract: Fischer Indole Synthesis with Organozinc Reagents.
Authors: Haag Benjamin A. , Zhang Zhi‐Guang , Li Jin‐Shan , Knochel Paul 
Published: March 2011

AbstractPrimary and secondary alkylzinc reagents add to various aryldiazonium salts leading to polyfunctional indoles by means of a [3,3]‐sigmatropic shift and subsequent aromatization.
ChemInform Abstract: Highly Efficient Synthesis of Cyclic Carbonates from Epoxides Catalyzed by Indium Tribromide System.
Authors: Shibata Ikuya , Mitani Ikuko , Imakuni Akira , Baba Akio 
Published: March 2011

AbstractThe reaction of substituted epoxides with CO2 under mild conditions allows the preparation of title compounds with tolerance of a variety of functional groups.
ChemInform Abstract: Synthesis of 2‐Oxazolones and α‐Aminoketones via Palladium‐Catalyzed Reaction of β,β‐Dibromoenamides.
Authors: Chai David I. , Hoffmeister Laura , Lautens Mark 
Published: March 2011

AbstractThe reaction of β,β‐dibromoenamides bearing an ester group at the nitrogen with boronic acids leads to formation of oxazolones [cf.
ChemInform Abstract: Synthesis, Characterization and Biological Studies of Some Bioactive Thiazolotriazole Derivatives.
Authors: Kumsi Manjunatha , Poojary Boja , Lobo Prajwal Lourdes , Kumari Nalilu Suchetha , Chullikana Anoop 
Published: March 2011

AbstractA variety of new substituted thiazolo[3,2‐b]triazole derivatives incorporating the Ibuprofen moiety is synthesized based on the three‐component reaction of triazolethione (IV) with monochloroacetic acid and (het)aromatic aldehydes.