bluuebunny/crossref_metadata_2025_split huggingface dataset:
Using Host 28S Ribosomal RNA as a Housekeeping Gene for Quantitative Real‐Time Reverse Transcription‐PCR (qRT‐PCR) in Virus‐Infected Animal Cells
Authors: Xue Jian‐Li , Cheng Xiao‐Wen 
Published: November 2010

AbstractThe use of quantitative real‐time reverse transcription‐PCR (qRT‐PCR) for studying regulation of gene transcription requires an internal template‐loading control or a housekeeping gene to guarantee the validity of the data collection, analysis, and interpretation. Analysis of gene transcription in virus‐infected animal cells is problematic because virus infection often results in modified or fluctuating gene transcription patterns of conventionally used housekeeping genes, such as the glyceraldehyde 3‐phosphate dehydrogenase (GAPDH) gene and the β‐actin gene. It has been demonstrated that the host 28S ribosomal gene can be used as a housekeeping gene in qRT‐PCR in virus‐infected insect cells. The stability of the human 28S rRNA gene transcription during the infection of HeLa cells with adenovirus has been confirmed, and this method has been extended to the use of the human 28S rRNA gene as a housekeeping gene in adenovirus‐infected HeLa cells. Step‐by‐step instructions are described for use of this control in analysis of gene transcription in both types of virus‐infected animal cells. Curr. Protoc. Microbiol. 19:1D.2.1‐1D.2.13. © 2010 by John Wiley & Sons, Inc.
Laboratory Maintenance of Streptomyces argillaceus and Streptomyces griseus
Authors: Bosserman Mary A. , Shepherd Micah D. , Kharel Madan K. , Rohr Jürgen 
Published: November 2010

AbstractThis unit describes general protocols for the laboratory maintenance of Streptomyces argillaceus and griseus, including growth on solid and liquid media, as well as specific considerations for the type of medium to be used with these species. Curr. Protoc. Microbiol. 19:10E.2.1‐10E.2.4. © 2010 by John Wiley & Sons, Inc.
Genetic Manipulation of Streptomyces Species
Authors: Nybo S. Eric , Shepherd Micah D. , Bosserman Mary A. , Rohr Jürgen 
Published: November 2010

AbstractThis unit includes general protocols for the genetic manipulation of Streptomyces species, including genomic DNA isolation, genomic library preparation, intergeneric conjugation of Streptomyces with E. coli, generation and transformation of Streptomyces protoplasts, electroporation of Streptomyces mycelia, and colony PCR. Curr. Protoc. Microbiol. 19:10E.3.1‐10E.3.26. © 2010 by John Wiley & Sons, Inc.
Rapid Re(f)use: 3‐D Fabricated Positive Waste Ecologies
Authors: Joachim Mitchell 
Published: November 2010

AbstractMitchell Joachim calls for a radical revision of our approach to waste management. Rather than the low‐level recycling that goes on through municipal authorities, he advocates the proactive use of waste to regenerate our cities. This he illustrates with Terreform One + Terrefuge's Rapid Re(f)use and Homeway projects that aim ‘to capture, reduce and redesign New York's refuse infrastructure’. Copyright © 2010 John Wiley & Sons, Ltd.
Production of filled hydrogels by mechanochemically induced polymerization
Authors: Damm C. , Mallembakam M. R. , Voronov A. , Peukert W. 
Published: November 2010

AbstractSilica nanoparticles functionalized with polyvinylpyrrolidone (PVP) were obtained by the grinding/mechanical activation of quartz or nonfunctionalized silica nanoparticles in a stirred media mill in the presence of 1‐vinyl‐2‐pyrrolidone, as proven by Fourier transform infrared spectroscopy. The polymer layer thickness formed on the silica nanoparticles after 8 h of mechanical activation in the absence of polymerization initiators amounted to about 10 nm, as derived from shear rheology. The silica nanoparticles functionalized with the hydrophilic PVP by mechanochemical polymerization reaction were used as fillers for hydrogels based on poly(hydroxyethyl methacrylate) (polyHEMA). The water absorption, release properties, and mechanical properties of the polyHEMA–silica composites were measured as functions of the filler content and particle size of the filler. PolyHEMA samples containing 20 wt % of the functionalized silica particles exhibited a higher maximum water absorption than the unfilled polymer; this showed that the hydrophilic interface between the filler and the matrix improved the water absorption. The release of methylene blue from the polyHEMA–silica composites was governed by diffusion and was almost unaffected by the silica particles. The values for the storage modulus and loss modulus of the polyHEMA–silica composites increased with growing filler content. For constant filler content, the storage modulus increased with decreasing particle diameter of the filler; this showed that the reinforcing effect increased with the interface between the filler particles and the matrix polymer. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Rheometric study of chitosan/activated carbon composite hydrogels for medical applications using an experimental design
Authors: Venault A. , Vachoud L. , Bouyer D. , Pochat‐Bohatier C. , Faur C. 
Published: November 2010

AbstractComposite chitosan/activated carbon hydrogels were prepared with the vapor‐induced phase separation process. A rheometric study was performed with a factorial fractional design to determine the formulation and process parameters significantly influencing the mechanical properties of the gels. The results revealed that three factors played a key role in the storage modulus of the gels. According to the model, these factors could be classified with respect to their relative influence on the storage modulus in the following descending order: chitosan concentration > gel time of exposure to ammonia vapors > temperature of the reactor. Increasing these parameters led to an increase in the physical crosslinking density within the matrices and resulted in a reinforcement of the mechanical properties of the hydrogels. Two interactions were also shown to be significant and promoted the formation of supplementary junction zones within the matrix: the first one corresponded to the interaction between the chitosan concentration and the exposure time to ammonia vapors, and the second one concerned the interaction between the chitosan concentration and the temperature of the reactor. A second‐order model was obtained from statistical analysis. Because of the determination coefficient (89.4%) and the P value related to the lack of adjustment of the model (0.043), which was associated with a 95% confidence level, this model could be considered to be of good quality. Three gels were used to validate the model, and good accuracy was obtained. The maximum elastic modulus was obtained with the highest chitosan concentration [4% (w/v)], the highest temperature in the gelation chamber (50°C), and the longest time of exposure to ammonia vapors (24 h). © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Indication for oophorectomy during cytoreduction for intraperitoneal metastatic spread of colorectal or appendiceal origin
Authors: Evers D J , Verwaal V J 
Published: November 2010

Abstract

Background
The incidence of ovarian metastases at the time of peritoneal carcinomatosis, and the influence of such metastases on survival after cytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC), are unknown.

Methods
This retrospective analysis included 194 women subjected to CRS and HIPEC since 2001. The incidence of ovarian metastases, disease-free survival and disease-specific survival were analysed.

Results
The histological diagnosis was colorectal cancer carcinomatosis in 108 patients, peritoneal mucinous carcinomatosis (PMCA) in 23 and disseminated peritoneal adenomucinosis (DPAM) in 63. Ninety-nine patients underwent oophorectomy during the HIPEC procedure. Ovarian metastases were confirmed in at least 52 per cent of the patients. There was a significant difference in disease-free survival between women with or without ovarian metastases in both PMCA and DPAM groups (P = 0·044 and P = 0·010 respectively). No significant differences in survival were found in the group with colorectal cancer carcinomatosis.

Conclusion
When peritoneal carcinomatosis of colorectal or appendiceal origin is confirmed, at least 52 per cent of ovaries will have synchronous metastases. Disease-free survival after a HIPEC procedure for PMCA or DPAM is significantly lower in women with ovarian metastases. Oophorectomy during CRS for peritoneal carcinomatosis should be strongly considered.
Numerical Simulation of Dispersed Gas/Liquid Flows in Bubble Columns at High Phase Fractions using OpenFOAM® Part 1 – Modeling Fundamentals
Authors: Marschall H. , Mornhinweg R. , Kossmann A. , Oberhauser S. , Langbein K. , Hinrichsen O. 
Published: November 2010

AbstractZahlreiche Produkte der chemischen Industrie entstehen in Gas/Flüssig‐Reaktoren mit Blasenströmungen. Entsprechend groß ist das Interesse daran, diese Reaktorklasse zu optimieren. Zur Auslegung industrieller Gas/Flüssig‐Reaktoren sind möglichst genaue Informationen über Strömungsstruktur und ‐verhalten des vorliegenden Zwei‐ oder Mehrphasensystems nötig. Die für die Simulationsgüte wesentlichen Modelle für die zwei‐fluid‐modell‐basierte Simulation disperser Gas/Flüssig‐Strömungen in Blasensäulen bei hohen Gasgehalten werden erläutert.
Methanol Needs More Attention as a Fuel and Raw Material for the Future
Authors: Bertau M. , Offermanns H. , Menges G. , Keim W. , Effenberger F. X. 
Published: November 2010

AbstractZum Thema der zukünftigen Energieversorgung und zur Vermeidung des Treibhauseffektes sind in jüngster Zeit viele Stellungnahmen und Strategiepapiere von wissenschaftlichen Gesellschaften und Wissenschaftsorganisationen erschienen; die Gesamtschau des Themenkreises „Energie, Kraftstoffe, Chemierohstoffe und Klima”︁ fehlt in aller Regel und dem Recycling von CO2, also der stofflichen Nutzung, z. B. zur Herstellung von Methanol, wird weniger Aufmerksamkeit geschenkt als der Speicherung von CO2, (Carbon Capture and Storage, kurz CCS).
Molecular Packing in Self‐Assembled p‐n and n‐p‐n Heterostructure Co‐oligomers
Authors: Wan Junhua , Li Yibao , Ma Zhun , Li Tingcheng , Huang Wei 
Published: November 2010

AbstractThe molecular packing structure in the self‐assembled p‐n and n‐p‐n heterostructure oligomers (OT2O and T2O2) was investigated. The macroscopic properties of the two oligomers were systemically investigated by differential scanning calorimetry (DSC), polarized optical microscopy (POM) and X‐ray diffraction (XRD), which revealed that the 3D (three‐dimensional) assemblies of the two oligomers exhibited obvious differences. The two‐dimensional assemblies (2D) of them were identified by scanning tunneling microscopy (STM) on a highly oriented poyrolytic graphite (HOPG) surface. Well‐ordered monolayer assembly structures were fabricated by symmetric molecules (OT2Os), while the ordered dormain of asymmetric molecule (T2O2s) could not be clearly resolved. This system, as an excellent example, may provide guidance for the design of the novel p‐n heterostructure oligomer and polymer.
Synthesis, Crystal Structure, Theoretical Calculation, Specific Heat Capacity, and Thermodynamic Properties of 4,4′‐Bis(3‐N‐methoxyformyl thioureido)‐diphenyloxide
Authors: Zhang Xinyu , Huang Jie , Song Jirong , Xu Kangzheng , Ban Qixun 
Published: November 2010

Abstract4,4′‐Bis(3‐N‐methoxyformyl thioureido)‐diphenyloxide was prepared via reaction of 4,4′‐diaminodiphenyl alter with potassium sulfocyanate and ethyl chloroacetate in ethyl acetate. The single crystal of the title compound was cultured by slow evaporation method at room temperature. The crystal structure was determined with X‐ray diffractometer. It is a monoclinic crystal, space group C2/c with a=0.95911(19) nm, b=0.75922(15) nm, c=2.7161(5) nm, α=90°, β=97.675 (3) °, γ=90°, V=1.9601(7) nm3, Z=4, Dc=1.472 g·cm−3, F(000) =904, µ=0.311 cm−1, R1=0.0367, wR2=0.1408. The specific heat capacity of the title compound was determined with continuous Cp mode of mircocalorimeter. The thermal behavior of the title compound was studied under a non‐isothermal condition by DSC method.
Influence of the Synthesis pH on Sorption Behavior of Mesoporous MCM‐41 for Toxic Metal Ions
Authors: Sepehrian Hamid , Waqif‐Husain Syed 
Published: November 2010

AbstractTwo samples of mesoporous MCM‐41 have been prepared in acidic and basic conditions with cetyltrimethylammonium bromide (CTAB) as template and characterized by powder X‐ray diffraction and N2 adsorption‐desorption measurement to confirm the ordered mesoporous structure. Sorption of 21 toxic metal ions on these sorbents has been studied and discussed. The results show that distribution coefficient of Ba(II), Fe(III), Th(IV) on the synthesized MCM‐41 in basic condition and Cu(II), Pb(II), Zr(IV) on the synthesized MCM‐41 in acidic condition has been enhanced.
Heterocycle‐bridged and Conformationally Constrained Retinoids: Synthesis of 4‐(7,8,9,10‐Tetrahydro‐7,7,10,10‐tetramethyl‐4H‐benzo[6,7]chromeno‐ [4,3‐d]thiazole‐2‐yl)benzoic Acid
Authors: Lamei Navid , Foroumadi Alireza , Emami Saeed , Amini Mohsen , Shafiee Abbas 
Published: November 2010

AbstractRetinoids are a class of synthetic and natural compounds structurally related to retinoic acid. In a search for discovery of a new class of heterocycle‐bridged and conformationally constrained retinoids, here we report the synthesis of 4‐(7,8,9,10‐tetrahydro‐7,7,10,10‐tetramethyl‐4H‐benzo[6,7]chromeno[4,3‐d]thiazole‐2‐yl)benzoic acid (10) starting from 5,6,7,8‐tetrahydro‐5,5,8,8‐tetramethylnaphthalen‐2‐ol (13). Several approaches was attempted to obtain target compound 10. Structure elucidation of synthesized compounds has been made on the basis of elemental analysis and spectral data (1H NMR, IR and MS).
Synthesis and Antimicrobial Activity of Quinazolin‐4(3H)‐ones Incorporating Sulfonamido‐4‐thiazolidinone
Authors: Patel Navin B. , Patel Jaymin C. , Patel Virendra N. 
Published: November 2010

AbstractSome new derivatives 7‐chloro‐2‐[2‐(2,6‐dichlorophenyl)amino]benzyl‐3‐[4‐(2‐substituted phenyl‐4‐oxo‐ thiazolidin‐3‐yl)phenyl]sulfonamido‐quinazolin‐4(3H)‐ones 5a–5l were synthesized from 2‐[2‐(2,6‐dichloro‐phenyl)amino]phenyl acetic acid via acid chloride, benzoxazinone, amino quinazolin‐4(3H)‐one and Schiff base formation. The synthesized compounds were screened for in vitro antibacterial and antifungal activities by broth micro dilution method. Some of the Schiff base as well as 4‐thiazolidinone derivatives showed promising antibacterial activity while pronounced antifungal activity was observed against C. albicans.
Pyrrolidine‐based Dicationic Acidic Ionic Liquids: Efficient and Recyclable Catalysts for Esterifications
Authors: Liu Xiaofei , Zhao Yingwei , Li Zhen , Chen Jing , Xia Chungu 
Published: November 2010

AbstractA highly efficient and eco‐friendly esterification was achieved by a series of novel dicationic acidic ionic liquids (DAILs) containing double SO3H cations. High yields of the product esters were obtained by using 2 mol% of the DAILs as catalysts and DAIL I could be reused at least ten times without appreciable loss of its efficicency.
Solvent‐free Synthesis of Alkylaminophenols via Petasis Boronic Mannich Reaction in One Pot without Catalysts
Authors: Liu Ying , Wang Limin , Sui Yuanyuan , Yu Jianjun 
Published: November 2010

AbstractBy using solvent‐free and heat conditions, the Petasis boronic Mannich reaction of salicylaldehydes with various boronic acids and secondary amines without catalyst is described. The alkylaminophenols were obtained in moderate to good yields in a shorter reaction time than the traditional methods.
Study on Vapor‐Liquid Nucleation Rates for n‐Alcohols by Density Functional Theory
Authors: Wu Huijie , Li Xiaosen 
Published: November 2010

AbstractThe statistical associating fluid theory (SAFT) in conjunction with the Weeks‐Chandler‐Anderson (WCA) approximation for intermolecular interaction is employed to construct a non‐uniform equation of state (EOS) for n‐alcohols. The molecular parameters for methanol, ethanol, 1‐propanol, 1‐butanol, 1‐pentanol and 1‐hexanol are obtained by fitting to the experimental data of vapor‐liquid equilibria and then used to predict the nucleation rates under the framework of density functional theory (DFT). The predictions are found to be in quite good agreement with the experimental data. Investigation shows that the combination of DFT and SAFT is a successful approach for vapor‐liquid nucleation rates of n‐alcohols.
Point Mutation Genotyping Based on Melting‐Point Difference without Fluorescent Probes
Authors: Meng Xuan , Huang Jianhua , Meng Xiangxian , Wu Yanqiong , Mo Qiuhua 
Published: November 2010

AbstractFluorescent method is a powerful tool for genotyping point mutations. Current existing methods usually need cost labeled fluorescent probes, which causes great limitation to their applications in clinic. Here, a fidelity method for genotyping point mutations has been developed based on melting‐point difference without labeled fluorescent probes. The method employs ligase to ligate two specific probes to produce a high melting temperature. The homozygotes and heterozygotes are scored accurately by obvious melting‐point difference among genotypes. This method would provide an accurate and economical‐cost tool for point mutation genotyping.
Mg Environments in the Octahedral Sheet of 2:1 Talc‐Like Hybrid Phyllosilicates: A Comparative XAFS Study
Authors: Miehé‐Brendlé Jocelyne , Tuilier Marie‐Hélène , Marichal Claire , Gallego Jean‐Christophe , Reinholdt Marc 
Published: November 2010

AbstractPhenethyl and Phenylaminomethyl talc‐like hybrid (TLH) materials prepared by a one‐step synthetic procedure are investigated by Mg K‐edge X‐ray absorption fine structure (XAFS) to obtain structural information on the octahedral sheet of these 2:1 phyllosilicates. A comparison of Mg K‐edge X‐ray absorption spectra of the talc‐like hybrids, synthetic montmorillonite containing 20 % Mg and 80 % Al in the octahedral sheet, and MgO is presented. Three peaks are observed in the near‐edge XAFS spectra of the phyllosilicates. Their interpretation is drawn from previous multiple scattering, band structure, and molecular cluster simulations of electron energy loss near‐edge structures spectra of MgO. Among the theoretical approaches found in the literature, the molecular cluster calculations highlight the dependence of the observed features on Mg–O and Mg–Mg orbital overlaps. Our results reveal a lower Mg–Mg orbital overlap in the hybrid materials than in MgO. The extended XAFS analysis emphasises a deviation from the regular Mg octahedral coordination in the TLHs. Two different Mg–O distances are identified; the shorter one is found to be significantly higher than predicted in the theoretical talc structure.
Visual recognition memory: A double anatomo‐functional dissociation
Authors: Barbeau Emmanuel J. , Pariente Jérémie , Felician Olivier , Puel Michèle 
Published: November 2010

AbstractThere is an ongoing debate regarding the respective role of anterior subhippocampal structures and the hippocampus in recognition memory. Here, we report a double anatomo‐functional dissociation observed in two brain‐damaged patients, FRG and JMG. They both suffered from complete destruction of left MTL structures. In the right hemisphere however, FRG sustained extensive lesions to the hippocampus sparing anterior subhippocampal structures, while JMG suffered from the reversed pattern of lesion, i.e., extensive damage to anterior subhippocampal structures but preserved hippocampus. FRG was severely amnesic and failed all recall tasks involving visual material, but exhibited normal performance at a large battery of visual recognition memory tasks. JMG was not amnesic and showed the opposite pattern of performance. These results strongly support the view that right anterior subhippocampal structures are a critical relay for visual recognition memory in the human. © 2010 Wiley‐Liss, Inc.
NMDA receptor hypofunction in the dentate gyrus and impaired context discrimination in adult Fmr1 knockout mice
Authors: Eadie Brennan D. , Cushman Jesse , Kannangara Timal S. , Fanselow Michael S. , Christie Brian R. 
Published: November 2010

AbstractFragile X syndrome (FXS) is the most common form of inherited intellectual disability in humans. This X‐linked disorder is caused by the transcriptional repression of a single gene, Fmr1. The loss of Fmr1 transcription prevents the production of Fragile X mental retardation protein (FMRP) which in turn disrupts the expression of a variety of key synaptic proteins that appear to be important for intellectual ability. A clear link between synaptic dysfunction and behavioral impairment has been elusive, despite the fact that several animal models of FXS have been generated. Here we report that Fmr1 knockout mice exhibit impaired bidirectional synaptic plasticity in the dentate gyrus (DG) of the hippocampus. These deficits are associated with a novel decrease in functional NMDARs (N‐methyl‐D‐aspartate receptors). In addition, mice lacking the Fmr1 gene show impaired performance in a context discrimination task that normally requires functional NMDARs in the DG. These data indicate that Fmr1 deletion results in significant NMDAR‐dependent electrophysiological and behavioral impairments specific to the DG. © 2010 Wiley Periodicals, Inc.
Mu opioid receptor mRNA expression, binding, and functional coupling to G‐proteins in human epileptic hippocampus
Authors: Cuellar‐Herrera Manola , Velasco Ana Luisa , Velasco Francisco , Chavez Laura , Orozco‐Suarez Sandra , Armagan Guliz , Turunc Ezgi , Bojnik Engin , Yalcin Ayfer , Benyhe Sandor , Borsodi Anna , Alonso‐Vanegas Mario , Rocha Luisa 
Published: November 2010

AbstractMu opioid receptors (MOR) are known to be involved in seizure activity. The main goal of the present study was to characterize the MOR mRNA expression, binding, as well as G protein activation mediated by these receptors in epileptic hippocampus of patients with pharmacoresistant mesial temporal lobe epilepsy (TLE). In contrast with autopsy samples, hippocampus obtained from patients with mesial TLE demonstrated enhanced MOR mRNA expression (116%). Saturation binding experiments revealed significantly higher (60%)Bmaxvalues for the mesial TLE group, whereas theKdvalues were not statistically different. Although mesial TLE group demonstrated high levels of basal binding for the G proteins (136%), DAMGO‐stimulated [35S]GTPγS binding did not demonstrate significant alterations. In conclusion, our present data provide strong evidence that the epileptic hippocampus of patients with pharmacoresistant mesial TLE presents significant alterations in MOR. Such changes may represent adaptive mechanisms to compensate for other as yet unknown alterations. © 2010 Wiley Periodicals, Inc.