bluuebunny/crossref_metadata_2025_split huggingface dataset:
Anti‐inflammatory activity of Eugenia punicifolia extract on muscular lesion of mdx dystrophic mice
Authors: Leite Paulo Emílio Corrêa , de Almeida Kessiane Belshoff , Lagrota‐Candido Jussara , Trindade Pablo , da Silva Rafael Ferreira , Ribeiro Manuel Gustavo L. , Lima‐Araújo Katia G. , Santos Wilson C. , Quirico‐Santos Thereza 
Published: November 2010

AbstractEugenia punicifolia known as “pedra‐ume caá” is a shrub largely distributed in the Amazon region popularly used in decoctions or infusions as a natural therapeutic agent, which can interfere on cholinergic nicotinic neurotransmission. This work aimed to investigate a putative anti‐inflammatory effect of dichloromethane fraction of E. punicifolia extract (Ep‐CM) in the muscular lesion of mdx dystrophic mice, considering that activation of cholinergic mechanisms mitigates inflammation. A polymer containing the Ep‐CM was implanted in mdx gastrocnemius muscle before onset of myonecrosis for local slow and gradual release of bioactive compounds and mice sacrificed 7 days or 9 weeks after surgery. Comparing to control muscle, treatment did not alter choline acetyltransferase and acetylcholinesterase enzymatic activities, but decreased metaloproteases‐9 and ‐2 activities and levels of tumor necrosis factor α and NFκB transcription factor. In addition, treatment also reduced levels of bioactive IL‐1β form and cleaved caspase‐3, related to early events of cellular death and inflammatory activation and further increased myogenin expression without affecting collagen production which is associated with fibrosis. In vivo treatment of mdx dystrophic mice with Ep‐CM caused significant reduction of muscular inflammation and improved skeletal muscle regeneration without inducing fibrosis. J. Cell. Biochem. 111: 1652–1660, 2010. © 2010 Wiley‐Liss, Inc.
Identification of functional domains and novel binding partners of STIM proteins
Authors: Saitoh Norimitsu , Oritani Kenji , Saito Kazunobu , Yokota Takafumi , Ichii Michiko , Sudo Takao , Fujita Natsuko , Nakajima Koichi , Okada Masato , Kanakura Yuzuru 
Published: November 2010

AbstractWith a signal trap method, we previously identified stromal interaction molecule (STIM: originally named as SIM) as a protein, which has a signal peptide in 1996. However, recent works have accumulated evidences that STIM1 and STIM2 reside in endoplasmic reticulum (ER) and that both mainly sense ER Ca2+ depletion, which plays an essential role in store operated calcium entry. In the present study, we extensively analyzed the domain functions and associated molecules of STIMs. A STIM1 mutant lacking the coiled‐coil domains was massively expressed on the cell surface while mutants with the coiled‐coil domains localized in ER. In addition, STIM1 mutants with the coiled‐coil domains showed a longer half‐life of proteins than those without them. These results are likely to indicate that the coiled‐coil domains of STIM1 are essential for its ER‐retention and its stability. Furthermore, we tried to comprehensively identify STIM1‐associated molecules with mass spectrometry analysis of co‐immunoprecipitated proteins for STIM1. This screening clarified that both STIM1 and STIM2 have a capacity to bind to a chaperone, calnexin as well as two protein‐transporters, exportin1 and transportin1. Of importance, our result that glycosylation on STIM1 was not required for the association between STIM1 and calnexin seems to indicate that calnexin might function on STIM1 beyond a chaperone protein. Further information concerning regulatory mechanisms for STIM proteins including the data shown here will provide a model of Ca2+ control as well as a useful strategy to develop therapeutic drugs for intracellular Ca2+‐related diseases including inflammation and allergy. J. Cell. Biochem. 112: 147–156, 2011. © 2010 Wiley‐Liss, Inc.
Adsorption and dissociation of NH3 on clean and hydroxylated TiO2 rutile (110) surfaces: A computational study
Authors: Chang Jee‐Gong , Chen Hsin‐Tsung , Ju Shin‐Pon , Chang Ching‐Sheng , Weng Meng‐Hsiung 
Published: November 2010

AbstractThe adsorption and dissociation of NH3 on the clean and hydroxylated TiO2 rutile (110) surfaces have been investigated by the first‐principles calculations. The monodentate adsorbates such as H3NTi(a), H2NTi(a), NTi(a), H2NO(a), HNO(a), NO(a) and HO(a), as well as the bidentate adsorbate, TiNTi(a) can be formed on the clean surface. It is found that the hydroxyl group enhances the adsorption of certain adsorbates on the five‐fold‐coordinated Ti atoms (5c‐Ti), namely H2NTi(a), HNTi(a), NTi(a) and TiNTi(a). In addition, the adsorption energy increases as the number of hydroxyl groups increases. On the contrary, the opposite effect is found for those on the two‐fold‐coordinated O atoms (2c‐O). The enhanced adsorption of NHx (x = 1 − 2) on the 5c‐Ti is due to the large electronegativity of the OH group, increasing the acidity of the Ti center. This also contributes to diminish the adsorption of NHx (x = 1 − 2) on the two‐fold‐coordinated O atoms (2c‐O) decreasing its basicity. According to potential energy profile, the NH3 dissociation on the TiO2 surface is endothermic and the hydroxyl group is found to lower the energetics of H2NTi(a)+HO(a) and HNTi(a)+2{HO(a)}, but slightly raise the energetic of TiNTi(a)+3{HO(a)} compare to those on the clean surface. However, the dissociation of NH3 is found to occur on the hydroxylated surface with an overall endothermic by 31.8 kcal/mol and requires a barrier of 37.5 kcal/mol. A comparison of NH3 on anatase surface has been discussed. The detailed electronic analysis is also carried out to gain insights into the interaction nature between adsorbate and surface. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2011.
Mechanism of methylacetylene bisselenation catalyzed by palladium complex from density functional study
Authors: Wang Meiyan , Cheng Lin , Wang Jinping , Wu Zhijian 
Published: November 2010

AbstractThe reaction mechanism of Pd(0)‐catalyzed methylacetylene bisselenation reaction is investigated by using the density functional method. The overall reaction mechanism involves the oxidative addition, insertion, and reductive elimination steps. The regioselectivity has been investigated for the methylacetylene insertion into PdSe bond of both cis and trans palladium complexes. It is found that the methylacetylene insertion into PdSe bond of the trans palladium complex using the substituted carbon atom attached to selenyl group is preferred among the four pathways of methylacetylene insertion processes. The electronic mechanisms on the methylacetylene insertion into PdSe bond are discussed in terms of the Frontier molecular orbital interactions. In addition, the influence of carbon monoxide on methylacetylene bisselenation was studied and found that the methylacetylene coordination and insertion into PdSe bond take place first generating the PdC bond, followed by CO insertion into the PdC bond. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010.
Raman spectroscopic study of the uranyl titanate mineral euxenite (Y,Ca,U,Ce,Th) (Nb,Ta,Ti)2O6
Authors: Frost Ray L. , Palmer Sara J. , Reddy B. Jagannadha 
Published: November 2010

AbstractRaman spectra of the uranyl titanate mineral euxenite were analysed and related to the mineral structure. A comparison is made with the Raman spectra of uranyl oxyhydroxide hydrates. The observed bands are attributed to the TiO and (UO2)2+ stretching and bending vibrations, as well as lattice vibrations of rare‐earth ions. The Raman bands of euxenite are in harmony with those of the uranyl oxyhydroxides. The mineral euxenite is metamict as is evidenced by the intensity of the UO stretching and bending modes, which are of lower intensity than expected, and with bands that are significantly broader. Copyright © 2010 John Wiley & Sons, Ltd.
Surface‐enhanced Raman spectra of dimethoate and omethoate
Authors: Guerrini Luca , Sanchez‐Cortes Santiago , Cruz Victor L. , Martinez Sonia , Ristori Sandra , Feis Alessandro 
Published: November 2010

AbstractSurface‐enhanced Raman scattering (SERS) spectra are presented and analyzed for two important organophosphate pesticides, dimethoate (DMT) and omethoate (OMT). Very detailed SERS spectra were obtained by aggregated Ag hydrosols, both in aqueous suspension and dried on a glass substrate. The SERS and ordinary Raman spectra of DMT do not resemble each other, suggesting that a chemical reaction immediately occurs when DMT is adsorbed onto the metal surface. We propose that the reaction product is OMT, which is the oxygen analog of DMT, on the basis of the Raman and SERS spectra of OMT. Further support is derived from the calculated Raman spectra of DMT and OMT. Minor wavenumber and intensity differences that are observed between the SERS spectra of DMT reaction product and those of OMT could be related to different metal/adsorbate interaction modes. The results can be useful in the development of new analytical methods for the determination of pesticide residues in food. Copyright © 2010 John Wiley & Sons, Ltd.
Soil‐vegetation relationships of three arid land plant species and their use in rehabilitating degraded sites
Authors: Chigani H. Kargar , Khajeddin S. J. , Karimzadeh H. R. 
Published: November 2010

AbstractIt is essential to consider soil characteristics when establishing vegetation in arid lands. In this study, the redundancy analysis (RDA) ordination method was used in this study to evaluate the habitats of Halocnemum strobilaceum, Limonium iranicum and Tamarix passerinoides growing in the Sejzy region, east of Isfahan. The study area was stratified into six ecologic‐floristic complexes. Stratified random sampling was then employed to collect soil and vegetation data from 1‐ha quadrats on nine sites. The soil samples were analysed to determine the characteristics of the soil in which the three species grew and to examine the soil profiles up to a depth of 2 m. Soil analyses revealed that high amounts of gypsum, silt, clay, saline, alkali and sulphate in the soil constituted a favourable habitat for Halocnemum strobilaceum. However, the per cent of silt and sulphate concentration in deep layers were negatively correlated with the growth of this species. Evaluation of the L. iranicum habitat revealed that the amounts of carbonate and CCE in the topsoil were positively correlated with the establishment and growth of the species but salinity and alkalinity were negatively correlated. The primary soil characteristics that positively influenced T. passerinoides included the presence of organic matter and bicarbonate ions in the soil layers and the total Ca and Mg content of the topsoil. However, Na was negatively correlated with Tamarix species. Determination of the soil characteristics that are associated with each of these xerophytic species can be used to determine which species is suitable for rehabilitating degraded sites in the study area. Copyright © 2010 John Wiley & Sons, Ltd.
An alternative approach to estimating parameters in creep models of high‐density polyethylene
Authors: Cheng Joy J. , Polak Maria Anna , Penlidis Alexander 
Published: November 2010

AbstractPolyethylene (PE) is increasingly used in structural applications due to its light weight and rust‐resistant nature. With growing demand for the use of PE as a structural material, there is a need for mathematical models that describe the mechanical behavior of this material. Curve fitting using a linear time‐dependent model is a common approach for modeling creep of PE at the macrostructural level. However, besides the point estimates of the model parameters and the (visual) fit of the model to experimental data, little else is learnt from the curve‐fitting approach. This work presents a rigorous statistical approach for modeling creep compliance of PE. Four high‐density PE resins used over a wide range of applications are studied. Linear viscoelastic modeling using the multi‐Kelvin element theory is examined in two forms: model linear in parameters and model nonlinear in parameters. With the application of valid statistical techniques, complex relationships between model parameters, largely unstudied before, are observed, such as evidence of a high degree of correlation among material parameters of the creep model. POLYM. ENG. SCI., 2011. © 2010 Society of Plastics Engineers.
Photodegradation of plasticized poly(vinyl chloride) stabilized by different types of thermal stabilizers
Authors: Xiang Xiuqin , Chen Shuangjun , Zhang Jun , Chai Ruidan 
Published: November 2010

AbstractPhotodegradation of plasticized poly(vinyl chloride) (PVC) stabilized by different thermal stabilizers including organic calcium complex and mercaptide organotin was investigated. Plasticized PVC sheets prepared by an open twin‐roller mill and plate vulcanizing machine were exposed to xenon‐arc light with the irradiance of 0.51 W/(m2·nm) at 65°C. A much better color stability displayed by mercaptide organotin than organic calcium complex has been confirmed by digital photos and color difference. This can be explained that the more effective mercaptide organotin minimizes the amount of thermal damage from processing thus favours subsequent UV weathering. Carbonyl index and decomposition activation energy (Ea1) obtained from attenuated total refection‐Fourier transform infrared spectra (ATR‐FTIR) and thermogravimetric (TG) analysis, respectively, further indicate that plasticized PVC sheets containing mercaptide organotin have more excellent UV resistance. Mechanical tests reveal that photodegradation of PVC is accompanied by the predominant process of chain scission on the surface of samples. POLYM. ENG. SCI., 2011. © 2010 Society of Plastics Engineers.
1/f noise in conducting channels of topological insulator materials
Authors: Hossain M. Zahid , Rumyantsev Sergey L. , Teweldebrhan Desalegne , Shahil Khan M. F. , Shur Michael , Balandin Alexander A. 
Published: November 2010

AbstractWe report results of investigation of the low‐frequency excess noise in device channels made from topological insulators – a new class of materials with a bulk insulating gap and conducting surface states. The thin‐film Bi2Se3 samples were prepared by the ‘graphene‐like’ mechanical exfoliation from bulk crystals. The fabricated four‐contact devices had linear current–voltage characteristics in the low‐bias regime |VSD| < 0.1 V. The current fluctuations had the noise spectral density SI ∼1/f for the frequency f < 10 kHz. The noise density SI followed the quadratic dependence on the drain–source current and changed from about ∼10−22 to 10−18 A2/Hz as the current increases from ∼10−7 to 10−5 A. The obtained data is important for planning transport experiments with topological insulators. We suggest that achieving the pure topological insulator phase with the current conduction through the ‘protected’ surface states can lead to noise reduction via suppression of certain scattering mechanisms. The latter has important implications for implementing the ultra‐low‐power and ultra‐low‐noise electronics.
Warping of triple line in the wetting of B4C by a Cu‐1 at.% Cr alloy
Authors: Lin Qiaoli , Shen Ping , Yang Longlong , Qiu Feng , Jiang Qichuan 
Published: November 2010

AbstractThe wetting of polycrystalline B4C by a molten Cu‐1 at.% Cr alloy was investigated at 1373 K using a modified sessile drop method. The triple line warped after the contact angle reached a certain degree. The mechanism for this unusual phenomenon was presumably ascribed to the large disparity in the wettability for the liquid on the CrB2 reaction layer and the precipitated graphite phase, as well as to their formation kinetics, rather than to the capillary flow driven by the surface tension gradient, as observed in the Cu/Si system. Copyright © 2010 John Wiley & Sons, Ltd.
Physiological measurements and analyses in motor sports: a preliminary study in racing kart athletes
Authors: Yamakoshi Takehiro , Matsumura Kenta , Yamakoshi Yasuhiro , Hirose Hajime , Rolfe Peter 
Published: November 2010

AbstractThe aims of this study were to assess methods for performing physiological measurements in motor sports, and to carry out a preliminary study in athletes participating in kart racing. The measurement of physiological variables in motor sports is practically challenging, largely due to the restricted space available for sensors and instrumentation and to movement artefacts from driver's operations and car vibration, hence the paucity of publications. We performed a preliminary study of amateur racing kart athletes to assess the performance of basic measurement apparatus and to collect preliminary data on the possible influences of gravity on cardiovascular activity. We measured the vector magnitude of acceleration (G), instantaneous heart rate using electrocardiography, blood pressure with a wrist sphygmomanometer, eardrum temperature (taken as a measure of core body temperature) with a radiation thermometer, and lap time. The instrumentation functioned satisfactorily during karting on a racing circuit. In all participants during driving, heart rate was maintained at approximately 150 beats · min−1. Time‐frequency analysis of all heart rate data was performed to evaluate cardiac control mechanisms and this suggested that the observed rise in heart rate could be due to sympathetic acceleration. Furthermore, while we do not have sufficient data to draw firm conclusions, it is suggested that the rise in heart rate could be related to the G stresses to which the drivers were subjected. Cross‐correlation analysis of the G and heart rate signals was performed in one participant and this showed a statistically significant correlation. We also found a statistically significant decrease in blood pressure (P<0.01) and a rise in eardrum temperature (P<0.01) immediately after the driving period. We conclude that although current sensors and instrumentation can allow basic monitoring of physiological variables in motor sport athletes, further developments are needed to allow more detailed investigations to be performed. Cardiovascular activity in response to G stresses warrants particular detailed investigation.
First Salivary Screening of Celiac Disease by Detection of Anti‐transglutaminase Autoantibody Radioimmunoassay in 5000 Italian Primary Schoolchildren
Authors: Bonamico Margherita , Nenna Raffaella , Montuori Monica , Luparia Rita Pia Lara , Turchetti Arianna , Mennini Maurizio , Lucantoni Federica , Masotti Donata , Magliocca Fabio Massimo , Culasso Franco , Tiberti Claudio 
Published: November 2010

ABSTRACTObjective:The high prevalence of celiac disease (CD) prompted us to evaluate a new, noninvasive disease screening strategy. The aim was to identify CD in 6‐ to 8‐year‐old children for a timely diagnosis, start gluten‐free diet (GFD) in compliant subjects, achieve the growth target, and prevent CD complications.Methods:Five thousand subjects were invited to participate in the study. Four thousand forty‐eight saliva samples were tested for anti‐tissue transglutaminase (tTG) immunoglobulin (Ig)A using a fluid‐phase radioimmunoprecipitation method. Positive children were tested for serum radioimmunoassay tTG IgA, enzyme‐linked immunosorbent assay tTG IgA, and anti‐endomysium IgA. Children confirmed as positive by serum assays underwent endoscopy with duodenal biopsies and, at the diagnosis of CD, were suggested to start GFD.Results:Consent was obtained from 4242 parents (84.8%) for the screening to be performed, and adequate saliva samples were collected from 4048 children (95.4%). Thirty‐two children were found to be salivary tTG IgA positive and 9 with borderline autoantibody levels. Thirty‐one of the 32 and 3 of the 9 subjects were also serum positive. Twenty‐eight children showed villous atrophy when undergoing intestinal biopsy, whereas 1 had Marsh 1 lesions; 3 children were suggested to start GFD without performing endoscopy. CD prevalence in the population investigated (including 19 CD known cases) was 1.16%. The ratio between screening‐detected patients and those diagnosed before the screening was 3:2. The ratio between symptomatic and asymptomatic patients was 1:1.6.Conclusions:We demonstrated that it is possible to perform a powerful, simple, well‐accepted, and sensitive CD screening using saliva. Until now, the compliance with GFD in children with CD has been optimal.
The Multivesicular Body-Localized GTPase ARFA1b/1c Is Important for Callose Deposition and ROR2 Syntaxin-Dependent Preinvasive Basal Defense in Barley
Authors: Böhlenius Henrik , Mørch Sara M. , Godfrey Dale , Nielsen Mads E. , Thordal-Christensen Hans 
Published: November 2010

AbstractHost cell vesicle traffic is essential for the interplay between plants and microbes. ADP-ribosylation factor (ARF) GTPases are required for vesicle budding, and we studied the role of these enzymes to identify important vesicle transport pathways in the plant–powdery mildew interaction. A combination of transient-induced gene silencing and transient expression of inactive forms of ARF GTPases provided evidence that barley (Hordeum vulgare) ARFA1b/1c function is important for preinvasive penetration resistance against powdery mildew, manifested by formation of a cell wall apposition, named a papilla. Mutant studies indicated that the plasma membrane–localized REQUIRED FOR MLO-SPECIFIED RESISTANCE2 (ROR2) syntaxin, also important for penetration resistance, and ARFA1b/1c function in the same vesicle transport pathway. This was substantiated by a requirement of ARFA1b/1c for ROR2 accumulation in the papilla. ARFA1b/1c is localized to multivesicular bodies, providing a functional link between ROR2 and these organelles in penetration resistance. During Blumeria graminis f sp hordei penetration attempts, ARFA1b/1c-positive multivesicular bodies assemble near the penetration site hours prior to the earliest detection of callose in papillae. Moreover, we showed that ARFA1b/1c is required for callose deposition in papillae and that the papilla structure is established independently of ARFA1b/1c. This raises the possibility that callose is loaded into papillae via multivesicular bodies, rather than being synthesized directly into this cell wall apposition.
Nutritional status and quality of life at varying degrees of clinical severity of chronic liver disease
Authors: Panagaria Namita , Varma Kanika , Nijhawan Sandeep , Rai R.R. 
Published: November 2010

PurposeThe purpose of this paper is to assess the nutritional status and quality of life in patients according to the clinical severity of liver cirrhosis.Design/methodology/approachA total of 151 cirrhotics were selected for study. Nutritional assessment was done by anthropometry, subjective global assessment, malnutrition universal screening tool, biochemical estimations and 72‐hour dietary recall. Quality of life was assessed by chronic liver disease questionnaire.FindingsChild's status was A/B/C in 29/68/59, respectively. Muscle and fat depletion was significantly higher in grade C patients as compared to grade A and B patients. Incidence of malnutrition was lowest in grade A patients (72.4 percent) and highest in grade C patients (90.74 percent) (p = 0.00). Calorie and protein intake was significantly lower in grade C patients compared to grade A and B patients (p = 0.00). Child Pugh score was found to be an independent risk factor for various nutritional and clinical parameters.Research limitations/implicationsThe data presented reflect the assessment of a very small cohort of these patients and should be done on a larger scale.Practical implicationsLooking into the high prevalence of malnutrition and the negative impact of disease severity, nutritional assessment of these patients should be done on a regular basis and nutritional therapy planned accordingly.Originality/valueData on malnutrition from India are scarce. This study is a small attempt to throw light on the nutritional status and its variation according to clinical severity of liver cirrhosis.