bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phylogenetic regionalization of the Pan‐Himalayan vascular flora
Authors: Liu Yun , Saqib Saddam , Lu Limin , Lai Yangjun , Hu Haihua , Peng Danxiao , Zaman Wajid , Zhao Lina , Liu Bing , Wang Qiang , Lu Anming , Hong Deyuan , Ye Jianfei , Chen Zhiduan 
Published: February 2025

AbstractPan‐Himalaya, including Himalaya and its neighboring areas, hosts a rich and unique flora that is crucial for understanding the evolutionary history of mountainous biodiversity. Phylogenetic regionalization can provide novel insights into biodiversity distribution patterns and their formation mechanisms. In this study, based on comprehensive species distribution data and a species‐level phylogenetic tree, we propose a phylogenetic regionalization scheme of the Pan‐Himalayan vascular flora comprising 15 zones, which can be grouped into five floristic subregions and three floristic regions (i.e., the West Himalayan [WH], the Southeast Himalayan [SEH], and the Northeast Himalayan [NEH] regions). Our results reveal that the SEH and NEH regions are closely clustered, which are then grouped with the WH region. The floras of these three regions exhibit distinct evolutionary histories and phylogenetic structures. The WH region presents a phylogenetically clustered flora with Euro‐Mediterranean affinities and the highest evolutionary uniqueness. The NEH region has the highest species richness and phylogenetic diversity, with most of its areas, especially at higher elevations, displaying phylogenetic clustering. In contrast, the SEH region exhibits a phylogenetically overdispersed flora, characterized by harboring species‐rich families with tropical, temperate, and alpine distributions, as well as relict plant lineages. This study provides valuable insights into the evolutionary history of the Pan‐Himalayan flora and informs the development of effective conservation strategies.
Exploring the experiences of hemochromatosis (HHC) patients who undergo therapeutic venesection at a blood collection agency
Authors: Thorpe Rachel , Masser Barbara , Gemelli Carley N. , Knight Elizabeth , Jos Carol , Bentley Peter J. , Maddern Jan , O'Donovan Justine , Kakkos Athina , Welvaert Marijke 
Published: February 2025

AbstractBackgroundPeople with hereditary hemochromatosis (HHC) require therapeutic phlebotomy on an ongoing basis. Little is known about the facilitators and barriers they experience in donating at a blood collection agency (BCA), nor how these impact their willingness to engage in an ongoing relationship with a BCA. This study explored the experiences of HHC donors undergoing therapeutic phlebotomy at the Australian Red Cross Lifeblood (Lifeblood) in Australia.Study Design and MethodsAll HHC donors who had made at least one donation at Lifeblood in the last 2 years were invited to complete a survey. In this paper, we report the findings on enablers and barriers to donating at Lifeblood, donor and patient identity, communication, knowledge of blood use and of plasma, interest in donating plasma, and engagement in positive word of mouth about donating.ResultsData were obtained from 4350 therapeutic donors. Responders identified more enablers than barriers to donating at Lifeblood and 61.8% reported that Lifeblood used their blood. Responders were more likely to identify as donors than patients, and those with a stronger donor than patient identity were significantly more likely to report that their blood was used, had greater interest in donating plasma, and reported engaging in more positive word of mouth about donating.DiscussionFindings indicate that BCAs can do more to educate donors with HHC about how their blood is used to help others. Doing so may help to retain them as donors and can be beneficial for the blood supply, as well as for the donors themselves.
The Impact of Team Composition on Student’s Sport Skill Development in Sport Education
Authors: Xie Lei , Xing Fuyan , Gao Rong , Gu Peidi 
Published: February 2025

Purpose: This study explores how diverse team compositions influence table tennis skill development in college students during a Sport Education (SE) season, considering gender and initial skill-level differences. Method: A quasiexperimental design included 64 college students, evenly split into control and experimental groups based on gender. Control group had heterogeneous teams, while experimental group had both heterogeneous and homogeneous teams. The 16-week SE season assessed backhand and forehand blocking skill improvement through two weekly sessions (45 min each). Results: Both groups exhibited skill development, with no significant gender differences. However, the experimental group demonstrated more comparable skill development across different skill levels compared with control group. Conclusion: The study underscores the positive impact of a design combining homogeneous and heterogeneous teams in SE on college students’ table tennis skill development. Educators are advised to consider context-specific factors, including sociocultural influences and sport skill complexity, when structuring team compositions within SE.
Long-term outcome in a cohort of 36 patients with sacral dural arteriovenous fistulae after endovascular embolisation or microsurgery
Authors: Duan Yu , Qin Xuanfeng , Chen Qinyi , Xu Binbin , An Qinzhu , Liao Yujun , Hu Yuanyuan , Chen Gong 
Published: February 2025

ObjectiveSacral dural arteriovenous fistula (SDAVF) is a rare spinal vascular malformation and often misdiagnosed or even mistreated. This study delved into the clinical characteristics, vascular architecture and treatment results of SDAVF, with the goal of enhancing upcoming diagnostic and therapeutic methodologies.MethodsFrom March 2014 to March 2022, consecutive patients with SDAVF were retrospectively analysed. The data on demographics, symptom resolution, angioarchitectural features and postoperative course were studied. Spinal cord function was evaluated by modified Aminoff–Logue scale.ResultsA total of 36 patients with 36 SDAVFs were enrolled, 12 of whom were misdiagnosed on their initial visit. The SDAVFs were located at S1 in 24 (66.7%), S2 in 10 (27.8%) and S3 in 2 (5.6%) cases, respectively. The primary feeding arteries included lateral sacral artery (LSA) of internal iliac artery (31/36, 86.1%), the branches of external iliac artery (2/36, 5.6%) and median artery (3/36, 8.3%), most of which are straight. Venae terminalisis is the sole drainage vein, flowing back into perimedullary venous network. Endovascular embolisation is the main therapy method for 30 cases, while the other 6 cases were treated with microsurgical fistulectomy. MRI tests showed that the abnormal vascular signals around the medulla disappeared, and the spinal cord oedema was alleviated in the majority of cases (32/36, 88.9%). Six patients, who all were treated by endovascular embolisation at first time, had residual or recurrent and two of them were performed by microsurgical fistulectomy again. All patients by microsurgical fistulectomy had no residual or recurrent during follow-up. According to the spinal cord functional assessment, the Aminoff-Logue score was significantly decreased (Z=−3.449, p=0.001) postoperatively.ConclusionThe misdiagnosis rate of SDAVF is very high. The most feeding artery of SDAVF came from the LSA, which was thicker and more straight, making it easier for microcatheters to reach the fistula site. So, endovascular embolism has become the first choice of treatment with minimal invasion, and safe and effective results.
Sweet-liking and sugar supplementation as innovative components in substance use disorder treatment: A systematic review
Authors: van Amsterdam Jan , van den Brink Wim 
Published: February 2025

Objective: Substance use disorders are a major global public health concern. While a wide range of psychotherapies and pharmacotherapies are available for their treatment, efficacy is limited and many patients fail to benefit from these treatments. Like addictive substances, sugar seems to trigger the dopaminergic reward centre, and sweet-liking might be a modifier of substance use disorder treatment. Method: Systematic review to summarize the role of sugar and sugar-liking in addiction and addiction treatment. Results: Evidence from both preclinical and clinical studies suggests that a certain portion of the population has a genetic predisposition for sweet-liking, which might be related to a higher risk for substance use and dependence. Regarding nicotine dependence, glucose supplementation prior to or during smoking cessation rapidly mitigates withdrawal symptoms and increases smoking abstinence rates during nicotine replacement therapy. In alcohol dependence, sweet-liking patients encounter more challenges in achieving abstinence than sweet-disliking patients. In addition, sweet-liking patients with high cravings demonstrate higher abstinence rates than sweet-disliking patients. Finally, sweet-liking is associated with successful outcomes of naltrexone treatment in patients with an alcohol use disorder. Conclusion: These findings present promising new challenges and opportunities to fine-tune and optimize treatment protocols in addiction care.
Urine exosomal lncRNAs as novel biomarkers for early diagnosis of bladder cancer based on microarray differential expression profiling
Authors: Li Jun , Zhao Liming , Li Luning , Wang Xiaohua , Gao Yisheng , Gao Yongli , Wang Jinfeng 
Published: February 2025

Purpose We aimed to exploit a urine exosomal long non-coding RNAs (lncRNAs) fingerprint to facilitate the early diagnosis of bladder cancer. Methods Microarray differential expression profiling of lncRNAs was for the first time employed in urine exosomes from 10 non-muscle-invasive bladder cancer (NMIBC) patients and 10 healthy controls to screen out candidate exosomal lncRNA biomarkers, which were then verified by quantitative real-time polymerase chain reaction in three independent phases including bladder cancer cells, culture fluid and 200 NMIBC participants. Logistic regression was performed to construct a diagnostic model—the diagnostic potency of which was assessed. Results The profile of three exosome-derived lncRNAs (CCDC148-AS1, XLOC_006419, and RP5-1148A21.3) was screened and further verified to be notably over-expressed in NMIBC patients and bladder cancer cell lines, and exhibited area under the receiver-operating characteristic curve values of 0.873, 0.825, and 0.834, respectively, in training, validation, and double-blind validation phases. The profile was superior to urinary cytology in discriminating NMIBC from healthy controls ( P < 0.0001). A significant correlation existed between a higher level of CCDC148-AS1 and a higher tumor grade ( P < 0.001), and up-regulated CCDC148-AS1 as well as XLOC_006419 were statistically related with tumor node metastasis stage ( P = 0.004 and P = 0.031, respectively). These three identified lncRNAs were confirmed to originate from bladder cancer cells and be packaged within exosomes, thus staying sufficiently stable in urine. Conclusions Tumor-originated urine exosomal lncRNAs, as fingerprint in NMIBC, exhibited satisfying clinical significance in early diagnosis of bladder cancer.
Wing movement path planning method during wing-fuselage docking process based on measured data and improved PSO-RRT* algorithm
Authors: Liu Lunqian , Qi Zhenchao , Tian Wei , Wang Ping , Hao Fang 
Published: February 2025

Wing-fuselage docking is one of the most crucial stages in the aircraft assembly process. In China, the process of wing-fuselage docking for large aircraft relies on experience and on-the-spot error detection during the docking process currently. However, the inefficiency of the approach makes it difficult to meet the demands of efficient production. Moreover, aircraft wings are susceptible to deformation, and various manufacturing errors can lead to different types of deformations. To address this problem, an inverse planning method for wing docking paths was developed, leveraging measured data and an enhanced PSO-RRT* algorithm. Firstly, a part model was reconstructed using measured point cloud data to depict manufacturing errors accurately. Then, the deformation occurring during the wing positioning process was simulated based on this model. Finally, improved PSO-RRT* algorithm was proposed and the inverse planning of wing docking path was executed. The experiment showed that the average absolute error between wing deformation simulation and measured values is 0.0015 mm. The maximum absolute error is 0.03182 mm. Finally, to ensure that the generated path can be used for the actual assembly process. A path smoothing method based on cubic spline interpolation was proposed, and the path was segmented.
Hypoxic human adipose mesenchymal stem cells-derived extracellular vesicles induce P311 expression and inhibit activation and injury of human brain microvascular endothelial cells
Authors: Zhang Yun , Zhao Hanghang , Su Yu , Yang Shudong , Kang Tao , Li Li 
Published: February 2025

Objective Stem cell therapy can modify angiogenic pathways. Neural protein 3.1 (P311) possesses the pro-angiogenic property. This study strived to explore the action and mechanism of human adipose mesenchymal stem cells (hADSCs) in human brain microvascular endothelial cell (hBMEC) injury by regulating P311. Methods The hADSCs of the 3rd passage were stained with oil red O, Alizarin red, and Alcian blue to assess adipogenic, osteogenic, and chondrogenic differentiation, followed by an analysis of immune phenotype via flow cytometry. After culturing hADSCs in hypoxic (5% oxygen) and normoxic (20% oxygen) conditions, extracellular vesicles (EVs) were extracted via ultracentrifugation, followed by morphology observation by microscopy, size distribution analysis via Nanoparticle tracking analysis, and surface marker determination by Western blot. hBMECs were treated with lipopolysaccharide (LPS) and cultured with normoxia or hypoxic hADSC-EVs. The effects of normoxia and hypoxic hADSC-EVs on proliferation, migration, and tube formation of hBMECs were assessed via CCK-8, Transwell, and tube formation assays. hBMECs were transfected with pcDNA3.0-P311 or P311 siRNA to evaluate the action of P311 on hBMEC injury. Results Hypoxic hADSC-EVs had a larger mean diameter, a wider diameter distribution range, and a higher particle concentration than normoxic hADSC-EVs. Hypoxia and normoxic hADSC-EVs were internalized by hBMECs, and hypoxic hADSC-EVs were more internalized. LPS suppressed hBMEC proliferation, migration, and tube formation and induced hBMEC injury. Hypoxia and normoxic hADSC-EVs ameliorated hBMEC injury, and hypoxic hADSC-EVs were superior to normoxic hADSC-EVs. P311 overexpression mitigated hBMEC injury, whereas P311 knockdown partly averted hypoxic hADSC-EV-exerted suppression on hBMEC injury. Conclusion Hypoxic hADSC-EVs can protect against LPS-induced hBMEC injury by upregulating P311.
Efficiency of Cephalic Vein Only Anastomosis During Radial Forearm Free Flap
Authors: Mousa-Doust Dorsa , Tran Khanh Linh , Bahat-Dinur Anat , Kwon Jamie Jae Young , Deane Emily C. , Anderson Donald W. , Durham J. Scott , Prisman Eitan 
Published: February 2025

Importance The closure technique for the radial forearm free flap (RFFF), a commonly utilized flap in head and neck reconstruction, remains a debated topic as there are unique advantages and drawbacks to each technique. Objective The present study aims to report on the outcomes of the closure of the RFFF with the superficial cephalic vein (CV)-only system in terms of venous compromise and flap survival. Methods (Design, Setting, Participants, Intervention, Measures) A retrospective review of patients who underwent head and neck reconstruction with RFFF between January 2015 and May 2021 at the authors’ institution was performed. Cases were categorized as superficial, dual, and deep systems. Multiple population and operative variables were collected for the study group. Results In total, 221 cases of RFFF were included, of which 169 (76.5%) cases were performed using the CV alone, whereas the remaining 52 (22.5%) cases utilized either the dual or the deep system alone. The operative time in the superficial group was 265 minutes. There were 9 venous complications in the CV group, of which 5 required re-exploration in the operating room and 4 were treated conservatively. All venous-related flap complications were salvaged. Conclusion and Relevance A majority of the RFFF cases could successfully be completed using the CV as the sole venous drainage, with high rates of flap survival, low complications, and low operative time.
Process evaluation of technologically assisted senior care using mixed methods: Results of the virtual assisted living (VAL, German: VBW Virtuell Betreutes Wohnen) project
Authors: Jansen Eva , Supplieth Juliana , Lech Sonia , Zöllick Jan , Schuster Johanna 
Published: February 2025

Objective Technologically assisted support systems and social support in everyday life can help senior citizens live longer independently in their homes. The purpose of this process evaluation is to investigate an innovative care model integrating monitoring technology with social support services, aimed at enabling senior citizens to live independently and extend their longevity in their homes. Methods Data collection of this mixed-method study was conducted through three distinct sources: expert interviews with employees of the participating social service, focus groups with seniors participating in the intervention, and involved consortium partners in the project. Following Kuckartz's methodology, we employed a structural qualitative content analysis using MAXQDA software. Additionally, a portion of the standardized survey administered post-intervention to participants was analyzed using descriptive statistics. Results The focus groups identified key challenges related to technical implementation such as false alarms and the failure of sensors as well as communication between invested parties. However, significant potential was noted in the practical execution of the intervention and social care. Interview participants emphasized the need for improved technical implementation. Results from the questionnaires indicate a generally positive perception of the intervention, particularly regarding its social dimensions. Conclusions Surveying individuals who implement and utilize assistive technology can yield valuable insights into its effectiveness. Additionally, it is crucial to comprehensively and in detail capture the experiences of those involved in testing new care models. Future research on assistive technologies for older adults should integrate both technical and social support components, while also addressing secure data protection measures and the paradox of reassurance.
Protocol for a usability and pilot implementation study of a digital medical device to assess pain in non-verbal people with dementia in Portuguese residential care facilities
Authors: Pereira Guerreiro Mara , Félix Isa Brito , Tomé Morgane , Hoti Kreshnik , Ramos Catarina , Dias Beatriz , Andrade Teresa , Brito José , Hughes Jeff 
Published: February 2025

Objective People living with moderate to severe dementia (PLWD) are often unable to self-report pain. This matter is of particular concern given that up to 80% experience chronic pain. Mistreated or untreated pain in PLWD is associated with symptoms such as agitation and aggression, and unnecessary use of antipsychotic agents. Further, it can also engender mental burden in formal caregivers. The PainChek® App, a regulatory cleared class I medical device, enables the assessment and monitoring of pain in people who cannot verbalise it, such as those with moderate to severe dementia. To date there are no data on the real-world use of the PainChek® App in Portugal. To address this gap, we report the protocol of a pilot study, which combines usability evaluation and implementation research. Methods Usability evaluation of the PainChek® (Portuguese) App will be guided by the ISO framework, focused on effectiveness, efficiency and user satisfaction. Implementation research will combine qualitative interviews to inform the implementation process, a longitudinal study of formal caregivers’ psychological variables, implementation outcomes, plus qualitative interviews to explore the ‘hows’ and ‘whys’ of implementation. The NASSS framework will be used as an implementation framework, together with the COM-B model and the Theoretical Domains Framework. Results The usability and implementation studies have received ethics approval from the Egas Moniz Ethics Committee, under numbers 1367 and 64/24, respectively. Conclusion This study is expected to inform the scale-up of the PainChek® (Portuguese) App in real-world settings and establish a foundation for a larger effectiveness and implementation study.
Development of an evidence-informed implementation strategy for a digital supportive care platform for brain tumour patients, their carers and healthcare professionals
Authors: Schadewaldt Verena , O’Brien Teresa , Kalla Mahima , Krishnasamy Meinir , Burns Kara , Bray Sarah CE , Gilbert Cecily , De Abreu Lourenco Richard , Thomas Joseph , Capurro Daniel , Chapman Wendy , Borda Ann , Dhillon Rana S , Whittle James R , Drummond Katharine J 
Published: February 2025

Background Implementation challenges of digital health solutions (DHSs) comprise complexities of behavioural change, resource limitation, inertia in existing systems, and failure to include consumer preferences. Understanding the factors which contribute to successful implementation of DHS is essential. We report the development of an implementation strategy for Brain Tumours Online (BT Online), a digital supportive care platform for patients with brain tumours, their carers and healthcare professionals. Aim To develop an evidence-informed implementation strategy for BT Online, considering the specific barriers and facilitators to implementing DHS for adults with a brain tumour and their carers and healthcare professionals. Methods A rapid review methodology was used to summarise factors relevant to implementation of DHS for people affected by cancer. Themes from the review were supported by implementation guidelines for DHS and the combined evidence informed the implementation strategy. Each theme was matched with specific steps for implementing BT Online. Results The rapid review identified 10 themes, namely, awareness of the new digital platform; institutional integration and support; data security, the quality, usability and accessibility of the platform; belief in the benefit of the platform; the need for holistic and tailored features; the timing of introducing the platform; engagement of healthcare professionals; and the re-definition of roles and workload. The themes were matched with 51 concrete implementation steps. Discussion The purpose of the strategy was to minimise risk of implementation failure, consider the specific context of care and generate a reference framework to evaluate BT Online prior to upscaling to use outside the research context. Our method contributes a novel approach of developing an evidence-informed rigorous implementation strategy if existing implementation frameworks do not apply.