bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Significance of Gelatin in Calcium Phosphate Hybrid Bone Cement for Attachment and Differentiation of MG63 Cells
Authors: Ding Shinn‐Jyh , Shie Ming‐You 
Published: March 2011

AbstractNatural polymeric gelatin can improve the handling properties of self‐setting CPCs, which are used in the repair of bone defects. This study is aimed at examining the effect of gelatin on the biological functions of MG63 osteoblast‐like cells. Gelatin‐containing CPCs show greater cellular viability than a CPC control without gelatin and significantly more FN is absorbed. Cell proliferation, with the amount of doped gelatin, and the expression of activated FAK and total integrin, with increasing gelatin content, are increased. The highest levels of total integrin, pFAK, FN, osteocalcin, bone sialoprotein, and calcium deposits are expressed by MG63 cells on the CPC containing 10% gelatin.
Influence of the Active Particles on the Self‐Healing Efficiency in Glassy Matrix
Authors: Coillot Daniel , Méar François O. , Podor Renaud , Montagne Lionel 
Published: March 2011

AbstractIn the present paper, we present the influence of the composition of the active particles on the self‐healing property in glass–particles (B, B4C, VB, V, or VC) composites. The thermogravimetric curves and oxidation kinetics at 700 °C of the active particles are reported. We show that the oxidation rate is higher with vanadium‐based particles than with boron‐base ones, mainly due to the formation of B2O3 that acts as a diffusion barrier to oxygen. In situ healing of cracks in composites was observed using high‐temperature environmental scanning electron microscopy (HT‐ESEM). The composition of the phases formed by reaction between the oxidation products and the glassy matrix is systematically determined. Self‐healing occurs as a consequence of oxidation of active particles, which leads to the formation of an oxide, such as V2O5 and/or B2O3 that is fluid enough to fill in the crack at high temperature. The crack healing is obtained whatever the nature of the active particle.
Real‐Time Investigation of Crystallization and Phase‐Segregation Dynamics in P3HT:PCBM Solar Cells During Thermal Annealing
Authors: Agostinelli Tiziano , Lilliu Samuele , Labram John G. , Campoy‐Quiles Mariano , Hampton Mark , Pires Ellis , Rawle Jonathan , Bikondoa Oier , Bradley Donal D. C. , Anthopoulos Thomas D. , Nelson Jenny , Macdonald J. Emyr 
Published: March 2011

AbstractCrystallization and phase segregation during thermal annealing lead to the increase of power‐conversion efficiency in poly(3‐hexylthiophene) (P3HT):[6,6]‐phenyl C61‐butyric acid methyl ester (PCBM) bulk‐heterojunction solar cells. An understanding of the length and time scale on which crystallization and phase segregation occur is important to improve control of the nanomorphology. Crystallization is monitored by means of grazing incidence X‐ray diffraction in real time during thermal annealing. Furthermore, the change in film density is monitored by means of ellipsometry and the evolution of carrier mobilities by means of field effect transistors, both during annealing. From the combination of such measurements with those of device performance as a function of annealing time, it is concluded that the evolution of microstructure involves two important time windows: i) A first one of about 5 minutes duration wherein crystallization of the polymer correlates with a major increase of photocurrent; ii) a second window of about 30 minutes during which the aggregation of PCBM continues, accompanied by an increase in the fill factor.
Local expression of interleukin‐27 ameliorates collagen‐induced arthritis
Authors: Pickens Sarah R. , Chamberlain Nathan D. , Volin Michael V. , Mandelin Arthur M. , Agrawal Hemant , Matsui Masanori , Yoshimoto Takayuki , Shahrara Shiva 
Published: March 2011

AbstractObjectiveTo determine the mechanism of action of interleukin‐27 (IL‐27) against rheumatoid arthritis (RA).MethodsAdenovirus containing IL‐27 transcript was constructed and was locally delivered into the ankles of mice with collagen‐induced arthritis (CIA). Progression of arthritis was determined in treated and untreated mice by measuring ankle circumference and through histologic analysis. IL‐17 and its downstream targets as well as cytokines promoting Th17 cell differentiation were quantified by enzyme‐linked immunosorbent assay in CIA mouse ankles locally expressing adenoviral IL‐27 as well as in control‐treated mouse ankles. Ankles from both treatment groups were immunostained for neutrophil and monocyte migration (macrophages in the tissue). Finally, vascularization was quantified by histology and by determining ankle hemoglobin levels.ResultsEctopic expression of IL‐27 in CIA mice ameliorated inflammation, lining hypertrophy, and bone erosion as compared with control‐treated CIA mice. Serum and joint levels of IL‐17 were significantly reduced in the IL‐27–treated group compared with the control‐treated group. Two of the main cytokines that induce Th17 cell differentiation and IL‐17 downstream target molecules were greatly down‐regulated in CIA mouse ankles receiving forced expression of IL‐27. The control mice had higher levels of vascularization and monocyte trafficking than did mice ectopically expressing IL‐27.ConclusionOur results suggest that increased levels of IL‐27 relieve arthritis in CIA mouse ankles. This amelioration of arthritis involves a reduction in CIA mouse serum and joint levels of IL‐17 and results in decreased IL‐17–mediated monocyte recruitment and angiogenesis. Hence, the use of IL‐27 may be a strategy for treatment of patients with RA.
Paper 3: EUROCAT data quality indicators for population‐based registries of congenital anomalies†
Authors: Loane Maria , Dolk Helen , Garne Ester , Greenlees Ruth ,   a EUROCAT Working Group
Published: March 2011

AbstractThe European Surveillance of Congenital Anomalies (EUROCAT) network of population‐based congenital anomaly registries is an important source of epidemiologic information on congenital anomalies in Europe covering live births, fetal deaths from 20 weeks gestation, and terminations of pregnancy for fetal anomaly. EUROCAT's policy is to strive for high‐quality data, while ensuring consistency and transparency across all member registries. A set of 30 data quality indicators (DQIs) was developed to assess five key elements of data quality: completeness of case ascertainment, accuracy of diagnosis, completeness of information on EUROCAT variables, timeliness of data transmission, and availability of population denominator information. This article describes each of the individual DQIs and presents the output for each registry as well as the EUROCAT (unweighted) average, for 29 full member registries for 2004–2008. This information is also available on the EUROCAT website for previous years. The EUROCAT DQIs allow registries to evaluate their performance in relation to other registries and allows appropriate interpretations to be made of the data collected. The DQIs provide direction for improving data collection and ascertainment, and they allow annual assessment for monitoring continuous improvement. The DQI are constantly reviewed and refined to best document registry procedures and processes regarding data collection, to ensure appropriateness of DQI, and to ensure transparency so that the data collected can make a substantial and useful contribution to epidemiologic research on congenital anomalies. Birth Defects Research (Part A), 2011. © 2011 Wiley‐Liss, Inc.
Expression and biophysical analysis of a triple‐transmembrane domain‐containing fragment from a yeast G protein‐coupled receptor
Authors: Caroccia Katrina E. , Estephan Racha , Cohen Leah S. , Arshava Boris , Hauser Melinda , Zerbe Oliver , Becker Jeffrey M. , Naider Fred 
Published: March 2011

AbstractStructural characterization of G protein‐coupled receptors (GPCRs) is hindered by the inherent hydrophobicity, flexibility, and large size of these signaling proteins. Insights into conformational preferences and the three‐dimensional (3D) structure of domains of these receptors can be obtained using polypeptide fragments of these proteins. Herein, we report the expression, purification, and biophysical characterization of a three‐transmembrane domain‐containing 131‐residue fragment of the yeast α‐factor receptor, Ste2p. Ste2p TM1–TM3 (G31–R161) was expressed as a TrpΔLE fusion protein in Escherichia coli. The expressed protein was subject to CNBr cleavage to remove the fusion tag and TM1–TM3 was purified by reverse‐phased HPLC. The cleavage product was isolated in yields of up to 20 mg per liter of culture in both unlabeled and uniformly [15N]‐labeled and [15N, 13C, 2H]‐labeled forms. The secondary structure of TM1–TM3 was determined to be helical in a number of membrane mimetic environments, including 2,2,2‐trifluoroethanol (TFE):water and lysomyristoylphosphatidylglycerol (LMPG) detergent micelles by circular dichroism. Preliminary HSQC analysis in 50% TFE:water and LMPG micelles prepared in sodium phosphate and 4‐(2‐hydroxyethyl)‐1‐piperazine ethanesulfonic acid (HEPES) buffers revealed that this fragment is suitable for structural analysis by nuclear magnetic resonance (NMR). Complete backbone assignments and a detailed localization of the secondary structural elements of TM1–TM3 in 50% TFE:water have been achieved. © 2011 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 96: 757–771, 2011.
U1A protein‐stem loop 2 RNA recognition: Prediction of structural differences from protein mutations
Authors: Kormos Bethany L. , Pieniazek Susan N. , Beveridge David L. , Baranger Anne M. 
Published: March 2011

AbstractMolecular dynamics (MD) simulations were carried out to compare the free and bound structures of wild type U1A protein with several Phe56 mutant U1A proteins that bind the target stem loop 2 (SL2) RNA with a range of affinities. The simulations indicate the free U1A protein is more flexible than the U1A–RNA complex for both wild type and Phe56 mutant systems. A complete analysis of the hydrogen‐bonding (HB) and non‐bonded (VDW) interactions over the course of the MD simulations suggested that changes in the interactions in the free U1A protein caused by the Phe56Ala and Phe56Leu mutations may stabilize the helical character in loop 3, and contribute to the weak binding of these proteins to SL2 RNA. Compared with wild type, changes in HB and VDW interactions in Phe56 mutants of the free U1A protein are global, and include differences in β‐sheet, loop 1 and loop 3 interactions. In the U1A–RNA complex, the Phe56Ala mutation leads to a series of differences in interactions that resonate through the complex, while the Phe56Leu and Phe56Trp mutations cause local differences around the site of mutation. The long‐range networks of interactions identified in the simulations suggest that direct interactions and dynamic processes in both the free and bound forms contribute to complex stability. © 2011 Wiley Periodicals, Inc. Biopolymers 95: 591–606, 2011.
Criminalization of stalking in Italy: One of the last among the current European member states' anti‐stalking laws
Authors: De Fazio Laura 
Published: March 2011

AbstractThis article illustrates the state of knowledge on stalking in Italy from the first scientific review published in 2001 to the recent anti‐stalking law, which became effective in February 2009, introducing a new article in the existing Italian Penal Code. In recent years the interest in stalking has increased progressively, such that it is now possible to find official data on the prevalence of the phenomenon in Italy. At the same time, European research activity has moved from the recognition of the phenomenon to analysis of pathways for a victim's assessment of stalking risk and enactment of legal regulation. The new law is described and analyzed. Copyright © 2011 John Wiley & Sons, Ltd.
In Vitro Selection of Proteins that Undergo Covalent Labeling with Small Molecules by Thiol‐Disulfide Exchange by Using Ribosome Display
Authors: Yanagida Hayato , Matsuura Tomoaki , Kazuta Yasuaki , Yomo Tetsuya 
Published: March 2011

AbstractThere is a great deal of interest in proteins that can bind covalently to target molecules, as they allow unambiguous experiments by tight binding to molecules of interest. Here, we report the generation of proteins that undergo covalent labeling with small molecules through in vitro selection by using ribosome display. Selection was performed from a mutant library of the WW domain with a biotinylated peptide as its binding target, in which the biotin and the peptide are connected by a disulfide bond. After five rounds of selection, we identified mutants carrying a particular cysteine mutation. The binding target reacted specifically with the selected mutant, even in the presence of other proteins, and resulted in the generation of biotin‐ or peptide‐labeled WW domains by thiol–disulfide exchange. When the mutant was fused to a protein of interest, the fusion protein was also labeled with biotin. Thus, the characteristics of the selected mutant should be suitable as a tag sequence that can be covalently labeled with small synthetic molecules. These results indicate that the rapid and efficient generation of such proteins is possible by ribosome display.
Unprecedented Alkylzinc–Magnesium Alkoxide Clusters as Suitable Organometallic Precursors for Magnesium‐Containing ZnO Nanoparticles
Authors: Heitz Stephan , Aksu Yilmaz , Merschjann Christoph , Driess Matthias 
Published: March 2011

AbstractThe synthesis and characterization of the first heterobimetallic methylzinc–magnesium alkoxide clusters [Me6MgZn6(OR)8] [R=Et (1 a), n‐Pr (1 b), nBu (1 c)] with a bis‐cubane‐shaped MgZn6O8 core is described. The thermal degradation of 1 a–c and mixtures of 1 a and the homometallic MeZn–alkoxide cubane [{MeZnOtBu}4] (2) in dry synthetic air led to wide‐band‐gap semiconducting MgxZn1−xO nanoparticles at temperatures below 500 °C. The final materials were characterized by different analytical techniques such as PXRD, REM, TEM, EDX, and IR spectroscopy. The morphology of the as‐prepared magnesium‐containing ZnO samples is influenced by the different organo groups (alkoxo) in the precursors. EDX mapping showed that magnesium is uniformly distributed in the ZnO matrix. The incorporation of magnesium led to a distortion of the ZnO lattice with increased a axis and decreased c axis parameters. Room‐temperature photoluminescence (PL) spectra reveal that the near‐band‐edge (NBE) emission of Mg2+‐doped ZnO is shifted to higher energies relative to that of pure ZnO.
Synthesis of Polyfunctional Allenes by Successive Copper‐Mediated Substitutions
Authors: Schade Matthias A. , Yamada Shigeyuki , Knochel Paul 
Published: March 2011

AbstractReadily available 1,1‐dichloro‐2‐alkynes are versatile starting materials for the synthesis of polyfunctionalized allenes. They can be prepared from commercially available terminal alkynes in a two‐step procedure. The Cu‐mediated reaction of several 1,1‐propargylic derivatives with functionalized alkyl, benzylic, or allylic zinc reagents proceeds exclusively with SN2′ selectivity and allows a rapid and efficient synthesis of functionalized chloroallenes. These chloroallenes undergo a novel CuI‐catalyzed substitution reaction with functionalized arylmagnesium reagents with excellent SN2 selectivity to give trisubstituted polyfunctionalized allenes. The functional group tolerance is excellent and functionalities, such as cyano, keto, ester, phosphate, trifluoromethyl, and halogens, are well tolerated.
ChemInform Abstract: High‐Pressure Synthesis and Local Structure of Corundum‐Type In2‐2xZnxSnxO3 (x ≤ 0.7).
Authors: Hoel Cathleen A. , Amores Jose Manuel Gallardo , Moran Emilio , Alario‐Franco Miguel Angel , Gaillard Jean‐Francois , Poeppelmeier Kenneth R. 
Published: March 2011

AbstractIn2‐2xZnxSnxO3 solid solutions with 0.2 ≤ x ≤ 0.8 are synthesized from stoichiometric mixtures of In2O3, SnO2, and ZnO (70 kbar, 1000 °C, 30 min for x ≤ 0.7 and 1150 °C, 1 h for x = 0.8).
ChemInform Abstract: Effect of Inclining Strain on the Crystal Lattice Along an Extended Series of Lanthanide Hydroxysulfates Ln(OH)SO4 (Ln: Pr—Yb, Except Pm).
Authors: Zehnder Ralph A. , et al. et al. 
Published: March 2011

AbstractThe title compounds are hydrothermally prepared from aqueous solutions of Ln2(SO4)3 (Ln: Pr—Yb, except Pm) and NaOH (autoclave, 170 °C, 10 d).
ChemInform Abstract: Sulfates of the Refractory Metals: Crystal Structure and Thermal Behavior of Nb2O2(SO4)3, MoO2(SO4), WO(SO4)2, and Two Modifications of Re2O5(SO4)2.
Authors: Betke Ulf , Wickleder Mathias S. 
Published: March 2011

AbstractThe crystal structures of (II), (IV), (VI), and (VIII) are determined by single crystal XRD.
ChemInform Abstract: Structure‐Induced Ferromagnetic Stabilization in Free‐Standing Hexagonal Fe1.3Ge Nanowires.
Authors: Yoon Hana , Lee Alex Taekyung , Choi Eun‐Ae , Seo Kwanyong , Bagkar Nitin , Cho Jaehun , Jo Younghun , Chang K. J. , Kim Bongsoo 
Published: March 2011

AbstractThe title nanowires are synthesized for the first time by a chemical vapor transport process without any catalyst using a 1:1 Ge/C powder mixture and FeI2 as precursors (650→870 °C, 10 min).
ChemInform Abstract: Pyrolytic Decomposition of Ammonia Borane to Boron Nitride.
Authors: Frueh Samuel , Kellett Richard , Mallery Carl , Molter Trent , Willis William S. , King'ondu Cecil , Suib Steven L. 
Published: March 2011

AbstractThe thermal decomposition of NH3·BH3 is characterized by TGA, TPD‐MS, attenuated total reflectance FTIR, scanning Auger microscopy, powder XRD, FESEM, DSC, and TGA‐MS.
ChemInform Abstract: Direct Preparation of LiBH4 from Pre‐Treated LiH + B Mixture at High Pressure.
Authors: Chen Rugan , Wang Xinhua , Xu Lou , Li Hui , Chen Changpin , Chen Lixin , Pan Hongge 
Published: March 2011

AbstractLiBH4 is prepared by ball milling of LiH and B under 10 MPa H2 (10 h) followed by heating at 300—500 °C under 35 MPa H2 (10 h).
ChemInform Abstract: Ca2LiC3H: A New Complex Carbide Hydride Phase Grown in Metal Flux.
Authors: Lang David A. , Zaikina Julia V. , Lovingood Derek D. , Gedris Thomas E. , Latturner Susan E. 
Published: March 2011

AbstractThe new title compound is synthesized by reaction of carbon and CaH2 in a Ca/Li flux with a molar ratio of C/CaH2/Ca/Li of 6:1:10:10 (1323 K, 2 h, slow cooling).
ChemInform Abstract: [M(dap)3]InSb3S7 (M: Co, Ni): Two Novel Open‐Framework Thioindate—Thioantimonates with 8‐, 12‐, and 16‐Ring Intersecting Channels.
Authors: Zhou Jian , An Litao , Zhang Feng 
Published: March 2011

AbstractThe new open‐framework title compounds (dap: 1,2‐diaminopropane) are solvothermally synthesized from In, Sb, Co or Ni, S, dap, and H2O (sealed Pyrex tube, 170 °C, 15 d; 27% and 42% yield for M: Co resp.
ChemInform Abstract: Yttrium(III)‐Containing Tungstoantimonate(III) Stabilized by Tetrahedral WO42‐ Capping Unit, [{Y(α‐SbW9O31(OH)2) (CH3COO)(H2O)}3(WO4)] 17‐.
Authors: Ibrahim Masooma , Mal Sib Sankar , Bassil Bassem S. , Banerjee Abhishek , Kortz Ulrich 
Published: March 2011

AbstractNa16(NH4) [{Y(α‐SbW9O31(OH)2)(OAc) (H2O)}3(WO4)]·48H2O (I) is synthesized from a mixture of Na9[α‐SbW9O33], YCl3, and Na2WO4 in a LiOAc/AcOH buffer (pH: 5.3, 80 °C, 60 min; addition of NH4Cl solution, 23% yield).
ChemInform Abstract: Syntheses, Structures, and Magnetic Properties of Np3S5 and Np3Se5.
Authors: Jin Geng Bang , Skanthakumar S. , Haire Richard G. , Soderholm L. , Ibers James A. 
Published: March 2011

AbstractBlack prisms of the title compounds are prepared by reactions of stoichiometric mixtures of Np and S or Se in a CsCl flux at 1173 K for 4 d.
ChemInform Abstract: Homoleptic Coinage Metal Compounds of Group(IV)heteroborates.
Authors: Schubert Hartmut , Dimmer Joerg‐Alexander , Kuechle Fritz‐Robert , Eichele Klaus , Wesemann Lars 
Published: March 2011

AbstractThe crystal structures of (III), (V), (VII), and (IX) are determined by single crystal XRD.
ChemInform Abstract: Planar Symmetry Incompatibility in Ru—Sn—Zn Pseudo‐Decagonal Approximants Composed of Novel Pentagonal Antiprisms.
Authors: Xiong Ding‐Bang , Okamoto Norihiko L. , Inui Haruyuki 
Published: March 2011

AbstractThe new pseudo‐dodecagonal approximants Ru2Sn2Zn3 and Ru4.15Sn4.96Zn5.85 are synthesized from the elements (1273 K for 5 h and 923 K for 4 d) and characterized by single crystal XRD.
ChemInform Abstract: 1,4‐Addition of Silicon Dienoates to α,β‐Unsaturated Aldehydes Catalyzed by in situ‐Generated Silicon Lewis Acid.
Authors: Yanai Hikaru , Takahashi Arata , Taguchi Takeo 
Published: March 2011

AbstractThe reaction of furan (Ic) with aldehydes (VI) proceeds with high 1,4‐regioselectivity and good diastereoselectivity to afford anti‐adducts (VII) as main products.
ChemInform Abstract: Fast Oxidation of Secondary Alcohols by the Bromate—Bromide System Using Cyclic Microwave Heating in Acidic Water.
Authors: Paeaekkoenen Sanna , Pursiainen Jouni , Lajunen Marja 
Published: March 2011

AbstractUsing an elaborated microwave heating technique, secondary aliphatic and aromatic alcohols are rapidly oxidized in excellent yields by BrOH, generated in situ.
ChemInform Abstract: Oxidative Cleavage of Alkenes Using an in situ Generated Iodonium Ion with Oxone as a Terminal Oxidant.
Authors: Thottumkara Prem P. , Vinod Thottumkara K. 
Published: March 2011

AbstractVarious alkenes and diol (XVII) are subjected to the optimized conditions A) or B) using cheap and benign reagents.
ChemInform Abstract: Synthesis and Application of Poly(diallyldimethylammonium tribromide) as a Novel Polymeric Brominating Agent.
Authors: Hossein Mahdavi , Zahra Kachoie 
Published: March 2011

AbstractA new supported tribromide is used for the α‐bromoacetalization of ketones and for the bromination of alkenes and activated aromatic compounds.
ChemInform Abstract: BF3·OEt2‐Mediated Ring Opening of Oxetanes with Ylides Derived from the Phosphoniosilylation Products of Enones.
Authors: Jung Sun Ho , Jang Sung Yeun 
Published: March 2011

AbstractA four‐step, one‐pot procedure leads to products (III) and provides an efficient tool for the synthesis of β‐functionalized cyclic enones.
ChemInform Abstract: Highly Enantioselective Alkynylation of Trifluoropyruvate with Alkynylsilanes Catalyzed by the BINAP—Pd Complex: Access to α‐Trifluoromethyl‐Substituted Tertiary Alcohols.
Authors: Aikawa Kohsuke , Hioki Yuta , Mikami Koichi 
Published: March 2011

AbstractThe process is highly successful including the addition of polyynes such as (VI) and (VIII) to trifluoropyruvate (II).
ChemInform Abstract: Synthesis and Biological Evaluation of Novel N‐(Alkoxyphenyl)‐aminocarbonylbenzoic Acid Derivatives as PTP1B Inhibitors.
Authors: Tong Yuan Feng , Zhang Pei , Chen Feng , Hao Ling Hua , Ye Fei , Tian Jin Ying , Wu Song 
Published: March 2011

AbstractA series of novel N‐(alkoxyphenyl)‐aminocarbonylbenzoic acid derivatives (33 examples) is designed and synthesized.
ChemInform Abstract: TMSCl‐Promoted Selective Oxidation of Sulfides to Sulfoxides with Hydrogen Peroxide.
Authors: Bahrami Kiumars , Khodaei Mohammad M. , Yousefi Behrooz H. , Arabi Mehdi Seikh 
Published: March 2011

AbstractTmsCl as an additive promotes the chemoselective mono‐oxidation in high yields.
ChemInform Abstract: Sodium Dithionite Initiated Addition—Lactonization of 2‐Acylamino‐4‐pentenoic Acids with Fluoroalkyl Iodides to Synthesize 4‐Fluoroalkyl‐2‐acylamino‐4‐butyrolactones.
Authors: Yang Xueyan , Yang Xianjin , Zhu Shengjie , Ye Lingling , Chen Yang , Chen Guanlong , Wu Fanhong 
Published: March 2011

AbstractOptimum reaction conditions are studied.
ChemInform Abstract: Friedel—Crafts Acylation of Pyrroles and Indoles Using 1,5‐Diazabicyclo[4.3.0]non‐5‐ene (DBN) as a Nucleophilic Catalyst.
Authors: Taylor James E. , Jones Matthew D. , Williams Jonathan M. J. , Bull Steven D. 
Published: March 2011

AbstractThe method allows the synthesis of various derivatives including ester (XIV) as the immediate precursor of the antiinflammatory drug tolmetin (XV).