bluuebunny/crossref_metadata_2025_split huggingface dataset:
Multifunctional nanocomposite based on graphene oxide for in vitro hepatocarcinoma diagnosis and treatment
Authors: Shen Ai‐Jun , Li Dong‐Liang , Cai Xiao‐Jun , Dong Chun‐Yan , Dong Hai‐Qing , Wen Hui‐Yun , Dai Gong‐Hua , Wang Pei‐Jun , Li Yong‐Yong 
Published: May 2012

AbstractBecause of its unique chemical and physical properties, graphene oxide (GO) has attracted a large number of researchers to explore its biomedical applications in the past few years. Here, we synthesized a novel multifunctional nanocomposite based on GO and systemically investigated its applications for in vitro hepatocarcinoma diagnosis and treatment. This multifunctional nanocomposite named GO‐PEG‐FA/Gd/DOX was obtained as the following procedures: gadolinium‐diethylenetriamine‐pentaacetic acid‐poly(diallyl dimethylammonium) chloride (Gd‐DTPA‐PDDA) as magnetic resonance imaging (MRI) probe was applied to modify GO by simple physical sorption with a loading efficiency of Gd3+ up to 0.314 mg mg−1. In order to improve its tumor targeting imaging and treatment efficiency, the obtained intermediate product was further modified with folic acid (FA). Finally, the nanocomposite was allowed to load anticancer drug doxorubicin hydrochloride via π–π stacking and hydrophobic interaction with the loading capacity reaching 1.38 mg mg−1. MRI test revealed that GO‐PEG‐FA/Gd/DOX exhibit superior tumor targeting imaging efficiency over free Gd3+. The in vitro release of DOX from the nanocomposite under tumor relevant condition (pH 5.5) was fast at the initial 10 h and then become relatively slow afterward. Moreover, we experimentally demonstrated that the multifunctional nanocomposite exhibited obviously cytotoxic effect upon cancer cells. Above results are promising for the next in vivo experiment and make it possible to be a potential candidate for malignancy early detection and specific treatment. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A: 2499–2506, 2012.
In vivo evaluation of resorbable bone graft substitutes in mandibular sockets of the beagle
Authors: Shih Tsai‐Chin , Chang Wei‐Jen , Yang Jen‐Chang , Feng Sheng‐Wei , Lin Che‐Tong , Teng Nai‐Chia 
Published: May 2012

AbstractHydroxyapatite (Ca10(PO4)6(OH)2), with its high biocompatibility and good bioaffinity, stimulates osteoconduction and is slowly replaced by the host bone after implantation. However, clinical use of HA as a bone substitute has proved problematic. It is difficult to prevent dispersion of the HA granules and to mold the granules into the desired shape. Calcium sulfate as a bone graft substitute is rapidly resorbed in vivo releasing calcium ions, but fails to provide a long‐term, three‐dimensional framework to support osteoconduction. The setting properties of calcium sulfate, however, allow it to be applied in a slurry form, making it easier to handle and apply in different situations. This study examines the in vivo response of a (Hydroxyapatite, apatitic phase)/calcium sulfate dehydrate (CSD) composite using different ratios in the mandibular premolar sockets of the beagle. The HA (AP)/CSD composite materials prepared in ratios of 30/70, 50/50, and 70/30 were implanted into the mandibular premolar sockets for 5 and 10 weeks. The control socket was empty. The authors compared the radiographic properties and the changes in height and width of the mandibular premolar sockets in the beagle. The composite graft in the 30/70 ratio had the best ability to form new bones. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 100A:2726–2731, 2012.
Effects of calcium salts of acidic monomers on mineral induction of phosphoprotein immobilized to agarose beads
Authors: Ito Shuichi , Iijima Masahiro , Motai Fumiko , Mizoguchi Itaru , Saito Takashi 
Published: May 2012

AbstractThe aim of this study is to evaluate the mineralizing potential of acidic monomers and their calcium salts for mineralization, using anin vitromineral induction model. Phosvitin (PV) was used as a model phosphoprotein in this study. PV was immobilized on agarose beads with divinyl sulfone. Five aliquots of agarose‐immobilized PV, acidic monomers, and their calcium salts were incubated in mineralizing solution at various concentrations. The PV beads and acidic monomers were incubated at 37°C. Samples were taken at several time points during the incubation. Then, the agarose beads were analyzed for bound calcium by atomic absorption spectrometry. The mineral formed on the agarose beads was identified as an apatite by microarea X‐ray diffraction. Additionally, the specimens were observed using scanning electron microscopy (SEM). Mineral induction time decreased with increasing solution saturation. 4‐METCa salt [calcium salt of 4‐methacryloxyethyl trimellitate (CMET)] significantly reduced the mineral induction time. Using these data, the interfacial tension for mineral induction of PV and CMET was determined to be 90.1 and 92.7 ergs/cm2, respectively. The mineral induced in each specimen after incubation for 24 h was identified by its X‐ray diffraction pattern as apatite. SEM observation showed that lath‐shaped crystals were formed on the surfaces of the CMET. We conclude that CMET could play a role in dentin remineralization. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 100A:2760–2765, 2012.
Mesenchymal stem cells associated with porous chitosan–gelatin scaffold: A potential strategy for alveolar bone regeneration
Authors: Miranda Suzana C. C. C. , Silva Gerluza A. B. , Mendes Renato M. , Abreu Fernando Antônio M. , Caliari Marcelo V. , Alves José B. , Goes Alfredo M. 
Published: May 2012

AbstractTissue engineering has emerged as a novel treatment for replacement of lost bone tissue. This study evaluated the effects of a chitosan–gelatin scaffold seeded with bone marrow mesenchymal stem cells (BMMSCs) in the healing process of tooth sockets in rats. BMMSCs isolated from transgenic rats expressing enhanced green fluorescent protein (eGFP) were expanded and seeded on a chitosan–gelatin scaffold. These constructs were cultured for three days and characterized by scanning electronic microscopy (SEM) and energy dispersion spectroscopy (EDS). Receptor rats received the implant in the left sockets, after upper first‐molar extraction. Right alveoli served as control. Animals were sacrificed at days 5, 21, and 35 post‐graft for examination. Morphometry demonstrated increased bone mineralization after 21 and 35 days in transplanted sockets. Migration, differentiation, and fate of eGFP‐labeled BMMSCs were monitored by immunohistochemistry. Tartrate‐resistant acid phosphatase staining (TRAP) was carried out at 21 days, to identify the involvement of osteoclastic cells in the scaffold resorption. The biomaterial was resorbed by TRAP‐negative giant cells in a typical foreign body reaction. Immunohistochemical findings showed that BMMSCs contributed to bone, epithelial, and vascular repair. Together, results indicate that BMMSCs loaded in the chitosan–gelatin scaffold is a strategy for tissue development in bone engineering. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 100A:2775–2786, 2012.
Alginates induce differentiation and expression of CXCR7 and CXCL12/SDF‐1 in human keratinocytes—The role of calcium
Authors: Stenvik Jørgen , Sletta Håvard , Grimstad Øystein , Pukstad Brita , Ryan Liv , Aune Randi , Strand Wenche , Tøndervik Anne , Helge Torp Sverre , Skjåk‐Bræk Gudmund , Espevik Terje 
Published: May 2012

AbstractAlginates from seaweed are used in chronic wound management, though the molecular and cellular effects of various alginate dressings are not well documented. We have developed ultrapure sodium‐alginates from Pseudomonas fluorescens with different content and distribution of single guluronic acid (G) residues (0–45% G), and tested their biological activities on human primary keratinocytes (KCs). The alginates inhibited KC migration and induced expression of differentiation markers. The potency of the alginates correlated with the increasing percentage of single G residues. These findings were explained by different binding and release of ionic calcium (Ca++) from the alginates which subsequently triggered differentiation. Ca‐free alginates had no effect on KC migration and differentiation, but the chemokine receptor CXCR7 was upregulated. Q‐PCR revealed that also CXCL12/SDF‐1, one of two known CXCR7‐ligands, was induced by the alginates. Both CXCR7 and CXCL12‐induction was dependent on the alginate G‐content, and highest upregulation was induced by an alginate with 19% single G residues. In the epidermis, CXCR7 expression was restricted to the basal layer. This study defines two biological effects of ultrapure alginates on KCs, both being dependent on the alginate structure, and being either dependent or independent of Ca. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A 100A:2803–2812, 2012.
Influence of fourth generation poly(propyleneimine) dendrimers on blood cells
Authors: Ziemba Barbara , Halets Inessa , Shcharbin Dzmitry , Appelhans Dietmar , Voit Brigitte , Pieszynski Ireneusz , Bryszewska Maria , Klajnert Barbara 
Published: May 2012

AbstractDendrimers provide many exciting opportunities for potential biomedical applications. However, owing to their positively charged surfaces, poly(propyleneimine) (PPI) dendrimers show toxic and haemolytic activities. One of the methods for masking the peripheral cationic groups is to modify them using carbohydrate residues. In this study, three types of the fourth generation PPI dendrimers‐uncoated (PPI‐g4), approximately 35% maltotriose (Mal‐III)‐coated (PPI‐g4‐OS), and approximately 90% Mal‐III‐coated (PPI‐g4‐DS) were investigated by assessing their effects on red blood cell (RBC) haemolysis in samples of pure RBCs, RBCs in the presence of human serum albumin (HSA) or human plasma, and RBCs in whole blood. Lymphocyte proliferation and platelet (PLT) aggregation were also studied in the presence of various concentrations of dendrimers. Although all dendrimers examined affected all the blood cells studied, the unmodified PPI‐g4 had the most damaging effect. It caused high RBC haemolysis rates and PLT aggregation and greatly inhibited lymphocyte proliferation. These effects were caused by the cationic surface of this polymer. The modification of PPI‐g4 with Mal‐III reduced the effect of the dendrimer on all blood cells. The presence of HSA or plasma in the buffer containing the RBCs or RBC in whole blood significantly decreased the extent of dendrimer‐driven haemolysis. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:2870–2880, 2012.
Histological and molecular evaluation of iron as degradable medical implant material in a murine animal model
Authors: Mueller Peter P. , Arnold Sylvia , Badar Muhammad , Bormann Dirk , Bach Friedrich‐Wilhelm , Drynda Andreas , Meyer‐Lindenberg Andrea , Hauser Hansjörg , Peuster Matthias 
Published: May 2012

AbstractA small animal model was established to evaluate the potential of iron as a degradable implant material. After insertion into the tail of mice, the implants gradually degraded over a clinically relevant time period of several months. Histological analysis and gene expression data from whole‐genome microarray analyses indicated a limited inflammatory reaction. No evidence of cellular responses to excess iron ions was detected, suggesting that the iron degradation products were metabolically inactive. Iron‐rich compounds could be detected in the vicinity of the implant and in individual cells distant from the implantation site. These results demonstrate that the mouse model could be useful for the primaryin vivoevaluation of novel implant materials and that iron degradation products can accumulate in diverse organs of the body. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 100A:2881–2889, 2012.
β1‐adrenergic receptor autoantibodies from heart failure patients enhanced TNF‐α secretion in RAW264.7 macrophages in a largely PKA‐dependent fashion
Authors: Du Yunhui , Yan Li , Du Hongwei , Wang Li , Ding Fan , Quan Lin , Cheng Xiuli , Song Kai , Liu Huirong 
Published: May 2012

AbstractAutoantibodies against the second extracellular loop of β1‐adrenergic receptor (β1‐AA) not only contribute to increased susceptibility to heart failure, but also play a causative role in myocardial remodeling through their catecholamine‐like effects via binding with the β1‐adrenergic receptor. The current study was designed to determine whether β1‐AA isolated from the sera of heart failure patients could cause TNF‐α secretion from the murine macrophage‐like cell line RAW264.7. Blood samples were collected from 40 patients who had suffered heart failure, as well as from 40 healthy subjects. The titer of β1‐AA and the level of TNF‐α were detected using ELISA. The effect of β1‐AA on murine macrophage‐like cell line RAW264.7 proliferation was detected by CCK‐8 kits and CFSE assay. Western blot assay was used to analyze the expression of phospho‐VASP. β1‐AA appeared more frequently in patients with heart failure than in healthy subjects. The β1‐AA isolated from heart failure patients promoted an increase of TNF‐α levels, which could be completely blocked by the selective β1‐adrenergic receptor antagonist metoprolol and the second extracellular loop of β1‐adrenergic receptor (β1‐AR‐ECII), but only partially inhibited by PKA inhibitor H89. Furthermore, the β1‐AA could enhance the proliferation of RAW264.7 cells in vitro. Meanwhile, the expression of phospho‐VASP was markedly increased in the presence of β1‐AA. These results demonstrate for the first time that the β1‐AA isolated from heart failure patients could bind with β1‐AR on the surface of RAW264.7 cells, causing the release of TNF‐α largely in a PKA‐dependent fashion. J. Cell. Biochem. 113: 3218–3228, 2012. © 2012 Wiley Periodicals, Inc.
BMP2 induces osteoblast apoptosis in a maturation state and noggin‐dependent manner
Authors: Hyzy Sharon L. , Olivares‐Navarrete Rene , Schwartz Zvi , Boyan Barbara D. 
Published: May 2012

AbstractLarge doses of bone morphogenetic protein 2 (BMP2) are used clinically to induce bone formation in challenging bone defects. However, complications after treatment include swelling, ectopic bone formation, and adjacent bone resorption. While BMP2 can be effective, it is important to characterize the mechanism of the deleterious effects to optimize its use. The aim of this study was to determine the effect of BMP2 on apoptosis in osteoblast lineage cells and to determine the role of the BMP inhibitor Noggin in this process. Human mesenchymal stem cells (MSCs), immature osteoblast‐like MG63 cells, and mature normal human osteoblasts (NHOst) were treated with BMP2. A model system of increased endogenous BMP signaling was created by silencing Noggin (shNOG‐MG63). Finally, the BMP pathway regulating apoptosis in NHOst was examined using BMP signaling inhibitors (5Z‐7‐oxozeaenol, dorsomorphin, H‐8). Apoptosis was characterized by caspase‐3, BAX/BCL2, p53, and DNA fragmentation. BMP2 induced apoptosis in a cell‐type dependent manner. While the effect was minor in MSCs, MG63 cells had modest increases and NHOst cells had robust increases apoptosis after BMP2 treatment. Apoptosis was significantly higher in shNOG‐MG63 than MG63 cells. 5Z‐7‐oxozeaenol and dorsomorphin eliminated the BMP2‐induced increase in DNA fragmentation in NHOst, suggesting roles for TAB/TAK1 and Smad signaling. These results indicate that the apoptotic effect of BMP2 is dependent on cell maturation state, inducing apoptosis in committed osteoblasts through Smad and TAB/TAK1 signaling, and is regulated by Noggin. Dose and delivery must be optimized in therapeutic applications of BMP2 to minimize complications. J. Cell. Biochem. 113: 3236–3245, 2012. © 2012 Wiley Periodicals, Inc.
Epigenetic modulation by TFII‐I during embryonic stem cell differentiation
Authors: Bayarsaihan Dashzeveg , Makeyev Aleksandr V. , Enkhmandakh Badam 
Published: May 2012

AbstractTFII‐I transcription factors play an essential role during early vertebrate embryogenesis. Genome‐wide mapping studies by ChIP‐seq and ChIP‐chip revealed that TFII‐I primes multiple genomic loci in mouse embryonic stem cells and embryonic tissues. Moreover, many TFII‐I‐bound regions co‐localize with H3K4me3/K27me3 bivalent chromatin within the promoters of lineage‐specific genes. This minireview provides a summary of current knowledge regarding the function of TFII‐I in epigenetic control of stem cell differentiation. J. Cell. Biochem. 113: 3056–3060, 2012. © 2012 Wiley Periodicals, Inc.
Dihedral Hamiltonian Cycle Systems of the Cocktail Party Graph
Authors: Buratti Marco , Merola Francesca 
Published: May 2012

AbstractThe existence problem for a Hamiltonian cycle decomposition of (the so called cocktail party graph) with a dihedral automorphism group acting sharply transitively on the vertices is completely solved. Such Hamiltonian cycle decompositions exist for all even n while, for n odd, they exist if and only if the following conditions hold: (i) n is not a prime power; (ii) there is a suitable ℓ such that (mod 2ℓ) for all prime factors p of n and the number of the prime factors (counted with their respective multiplicities) such that (mod ) is even. Thus in particular one has a dihedral Hamiltonian cycle decomposition of the cocktail party graph on 8k vertices for all k, while it is known that no such cyclic Hamiltonian cycle decomposition exists. Finally, this paper touches on a recently introduced symmetry requirement by proving that there exists a dihedral and symmetric Hamiltonian cycle system of if and only if (mod 4).
Evaluation of Two Enzyme Immunoassays for the Detection of the Cocaine Metabolite Benzoylecgonine in 1,398 Urine Specimens
Authors: Carney Sarah , Wolf Carl E. , Tarnai‐Moak Lisa , Poklis Alphonse 
Published: May 2012

BackgroundBenzoylecgonine (BE) is the primary urinary metabolite of cocaine. Two enzyme immunoassays were evaluated for the detection of BEin urine with a 300 ng/ml cutoff: the DRI® Cocaine Metabolite Assay and Lin‐Zhi International's (LZ) Cocaine Metabolite Enzyme Immunoassay.MethodsThis study involved 1,398 urine specimens from criminal justice and pain management programs. Gas chromatography/mass spectrometry (GC/MS) data were obtained for presumptive positives, and for negative urine specimens yielding responses significantly above the negative control.ResultsApproximately 46% (644) of the specimens yielded positive results by DRI, and 47% (664) were positive by LZ. One specimen screened positive with both assays but was found to have a nondetectable BE concentration by GC/MS, indicating one false positive for each assay. Twenty‐one specimens yielding negative DRIresults contained BEabove 300 ng/ml, and 29 specimens yielded false negatives with the LZassay. Therefore, the overall agreement between both immunoassays and GC/MSresults was 98%. Assay sensitivity was 0.968 (DRI) and 0.958 (LZ); the selectivity for both assays was 0.999. Urine specimens containing cocaine, additional cocaine metabolites, and other drugs were also tested. No cross‐reactivity was observed.ConclusionBoth the DRIand LZassays provide a precise, reliable method for the routine detection of BEin urine. J. Clin. Lab. Anal. 26:130‐135, 2012. © 2012 Wiley Periodicals, Inc.
Significant Association of Elevated Concentration of Plasma YKL‐40 with Disease Severity in Patients With Pelvic Inflammatory Disease
Authors: Lee Shu‐Hsin , Lin Ching‐Yi , Wang Po‐Hui , Han Chih‐Ping , Yang Shun‐Fa , Chang Jinghua Tsai , Lee Meng‐Chih , Lin Long‐Yau , Lee Maw‐Sheng 
Published: May 2012

IntroductionTo date, no study reports the implication of YKL‐40 in pelvic inflammatory disease (PID). Therefore, we investigate the levels of plasma YKL‐40 in patients with PID and further associate its expression with the severity of disease.MethodsWe designed a hospital‐based case‐control study with approximate 1:1 ratio and consecutively recruited 64 patients with PID and 70 control women. We collected blood samples from 64 women with PID before and after they received treatment and 70 control women to detect levels of plasma YKL‐40 and C‐reactive protein (CRP) as well as white blood cell and neutrophil counts.ResultsThe results revealed that levels of plasma YKL‐40 were significantly elevated in patients with PID as compared to those in controls (38.36 vs. 21.69 ng/ml, P = 0.001) but the significant difference was restricted to women aged 30 years or old after age stratification (56.75 vs. 23.61 ng/ml, P ≤ 0.001). It declined significantly after they received treatment (median: 38.36 vs. 27.54 ng/ml; P ≤ 0.001). Although both plasma YKL‐40 and CRP were elevated in patients with tubo‐ovarian abscess, PID patients with surgery exhibited higher YKL‐40 concentration than those without surgery (median: 82.05 vs. 30.19 ng/ml, P = 0.005) and only plasma YKL‐40 was significantly associated with the length of the hospital stay (P ≤ 0.001, R = 0.604).ConclusionWe conclude that once individuals are diagnosed to have PID, YKL‐40 may act as a biomarker to predict the severity and clinical outcome of the disease. J. Clin. Lab. Anal. 26:136‐142, 2012. © 2012 Wiley Periodicals, Inc.
Identification of Novel Low Molecular Weight Serum Peptidome Biomarkers for Non‐Small Cell Lung Cancer (NSCLC)
Authors: Yang Juan , Song Yong‐Chun , Song Tu‐Sheng , Hu Xiao‐Yan , Guo You‐Min , Li Zong‐Fang , Dang Cheng‐Xue , Huang Chen 
Published: May 2012

AimTo identify discriminating protein patterns in serum samples among non‐small cell lung cancer (NSCLC), chronic obstructive pulmonary disease (COPD), pneumonia, and healthy controls. To discover specific low molecular weight (LMW) serum peptidome biomarkers and establish a diagnostic pattern for NSCLCby using proteomic technology.MethodsWe used magnetic bead‐based separation followed by matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry (MALDI‐TOF MS) to identify patients with NSCLC, COPD, and pneumonia. A total of 154 serum samples were analyzed in this study, among which there were 60 serum samples from NSCLC patients, 30 from patients with other lung‐related diseases (16 pneumonia patients and 14 patients with COPD) as disease controls, and 64 from healthy volunteers as healthy control. The mass spectra, analyzed using ClinProTools software, distinguished between cancer patients and healthy individuals based on GA algorithm model.ResultsIn this study, we generated numerous discriminating m/z peaks as well as disease‐specific discrimination peaks. A set of five potential biomarkers (m/z: 7,763.24, 1,012.61, 4,153.16, 1,450.55, and 2,878.89) could be used as the diagnostic biomarkers to distinguish NSCLCpatients from healthy controls. In the training set, patients with NSCLC could be identified with sensitivity of 97.5% and specificity of 98.8%. Similar results were obtained in the testing set, showing 80.7% sensitivity and 91.2% specificity.ConclusionOur study demonstrated that a combined application of magnetic beads with MALDI‐TOF MS technique was suitable for identification of serum biomarkers for NSCLC. J. Clin. Lab. Anal. 26:148‐154, 2012. © 2012 Wiley Periodicals, Inc.