bluuebunny/crossref_metadata_2025_split huggingface dataset:
Primary rat hepatocyte culture on 3D nanofibrous polymer scaffolds for toxicology and pharmaceutical research
Authors: Bierwolf Jeanette , Lutgehetmann Marc , Feng Kai , Erbes Johannes , Deichmann Steffen , Toronyi Eva , Stieglitz Christina , Nashan Bjoern , Ma Peter X. , Pollok Joerg M. 
Published: September 2010

AbstractPrimary rat hepatocytes are a widely used experimental model to estimate drug metabolism and toxicity. In currently used two‐dimensional (2D) cell culture systems, typical problems like morphological changes and the loss of liver cell‐specific functions occur. We hypothesize that the use of polymer scaffolds could overcome these problems and support the establishment of three‐dimensional (3D) culture systems in pharmaceutical research. Isolated primary rat hepatocytes were cultured on collagen‐coated nanofibrous scaffolds for 7 days. Cell loading efficiency was quantified via DNA content measurement. Cell viability and presence of liver‐cell‐specific functions (albumin secretion, glycogen storage capacity) were evaluated. The activity of liver‐specific factors was analyzed by immunofluorescent staining. RNA was isolated to establish quantitative real‐time PCR. Our results indicate that primary rat hepatocytes cultured on nanofibrous scaffolds revealed high viability and well‐preserved glycogen storage. Albumin secretion was existent during the entire culture period. Hepatocytes remain HNF‐4 positive, indicating highly preserved cell differentiation. Aggregated hepatocytes re‐established positive signaling for Connexin 32, a marker for differentiated hepatocyte interaction. ZO‐1‐positive hepatocytes were detected indicating formation of tight junctions. Expression of cytochrome isoenzymes was inducible. Altogether the data suggest that nanofibrous scaffolds provide a good in vitro microenvironment for neo tissue regeneration of primary rat hepatocytes. Biotechnol. Bioeng. 2011; 108:141–150. © 2010 Wiley Periodicals, Inc.
High‐throughput screening of a small molecule library for promoters and inhibitors of mesenchymal stem cell osteogenic differentiation
Authors: Brey Darren M. , Motlekar Nuzhat A. , Diamond Scott L. , Mauck Robert L. , Garino Jonathon P. , Burdick Jason A. 
Published: September 2010

AbstractThe use of high‐throughput screening (HTS) techniques has long been employed by the pharmaceutical industry to increase discovery rates for new drugs that could be useful for disease treatment, yet this technology has only been minimally applied in other applications such as in tissue regeneration. In this work, an assay for the osteogenic differentiation of human mesenchymal stem cells (hMSCs) was developed and used to screen a library of small molecules for their potential as either promoters or inhibitors of osteogenesis, based on levels of alkaline phosphatase activity and cellular viability. From a library of 1,040 molecules, 36 promoters, and 20 inhibitors were identified as hits based on statistical criteria. Osteopromoters from this library were further investigated using standard culture techniques and a wider range of outcomes to verify that these compounds drive cellular differentiation. Several hits led to some improvement in the expression of alkaline phosphatase, osteogenic gene expression, and matrix mineralization by hMSCs when compared to the standard dexamethasone supplemented media and one molecule was investigated in combination with a recently identified biodegradable and osteoconductive polymer. This work illustrates the ability of HTS to more rapidly identify potential molecules to control stem cell differentiation. Biotechnol. Bioeng. 2011; 108:163–174. © 2010 Wiley Periodicals, Inc.
The analysis of glycosylation: a continued need for high pH anion exchange chromatography
Authors: Behan Jennifer L. , Smith Kevin D. 
Published: September 2010

AbstractAn appreciation of the structures of the oligosaccharide chains which become attached to biomolecules (the process known as glycosylation), and their relevance to the biological function of the molecule concerned, has progressed rapidly in recent years with developments in site‐selective protein glycosylation, oligosaccharide synthesis and in vivo targeting of oligosaccharides. These developments have necessitated the parallel development of effective analytical tools for the determination of the structures of glycosylation. The conclusion of studies in the 1980s and 1990s was that high pH anion exchange chromatography (HPAEC) was the most effective HPLC mode for the analysis of glycosylation. It allowed the fractionation of complex mixtures of monosaccharides or oligosaccharides, the latter in terms of charge, size, composition, anomerity and intra‐chain linkages. This review reinvestigates whether HPAEC still appears to offer the most effective means of analysing glycosylation. Copyright © 2010 John Wiley & Sons, Ltd.
Description of a UK study of onset of sexually harmful behaviour before the age of ten years in boys referred to a specialist assessment and treatment service
Authors: Hawkes Colin 
Published: September 2010

AbstractDuring the past 30 years, research into the phenomenon of sexually harmful behaviour has shifted from an adult focus to include adolescent and, more recently, childhood onset. This paper reports a study of onset, before the age of ten years, of sexually harmful behaviour in a group of 27 boys.Ethical consent was obtained to extract information from the case files of a national specialist service. A description of characteristics was built up from variable frequencies and quantitative and qualitative analysis produced findings that indicated a family history of cross‐generational harm to children and a parental experience of unresolved harm in childhood generated inconsistent and insensitive parenting that was linked to high levels of maltreatment and insecurity of attachment in the research group. Sexualised reactions by the research subjects to a very high level of sexual victimisation were not responded to in a timely or appropriate way by parents, other caregivers or professionals so that sexually harmful behaviour continued without intervention for a significant period.The study proposes a three‐stage model that identifies predisposing vulnerabilities, sexual victimisation as a trigger event and the subsequent development of sexually harmful behaviour as a protecting adaptation that assumes an aroused and organised aspect through repetition. Copyright © 2010 John Wiley & Sons, Ltd.
Stress‐induced multiple organ damage in rats is ameliorated by the antioxidant and anxiolytic effects of regular exercise
Authors: Çakır Barış , Kasımay Özgür , Kolgazi Meltem , Ersoy Yasemin , Ercan Feriha , Yeğen Berrak Ç. 
Published: September 2010

AbstractOur aim was to investigate the effects of moderate load, regular swimming exercise on stress‐induced anxiety, and associated oxidative organ injury. Male Sprague‐Dawley rats (n = 48) were either kept sedentary or submitted to swimming exercise for 8 weeks. Rats were then divided as non‐stressed, acute stress, and chronic stress groups. After acute or chronic stress (electric foot shocks) applications, rats were placed on a holeboard and the exploratory behavior was recorded to assess the anxiety. Rats were decapitated after the stress application. Acute and chronic stress induction led to increased serum cortisol levels as compared to non‐stressed groups. Plasma aspartate aminotransferase levels that were elevated in sedentary rats with both stress exposures were lower in trained rats. Malondialdehyde levels and myeloperoxidase activity were increased in the cardiac muscle, liver, stomach, and brain of the stressed rats with a concomitant reduction in the glutathione levels, while stress‐induced changes in malondialdehyde, myeloperoxidase, and glutathione levels were reversed in the trained animals. Exercise, which led to increased malondialdehyde and reduced glutathione levels in the skeletal muscle of the non‐stressed rats, also protected against stress‐induced oxidative damage. Regular exercise with its anxiolytic and antioxidant effects ameliorates stress‐induced oxidative organ damage by a neutrophil‐dependent mechanism. Copyright © 2010 John Wiley & Sons, Ltd.
Intercatheter reproducibility of near‐infrared spectroscopy for the in vivo detection of coronary lipid core plaques
Authors: Abdel‐Karim Abdul‐Rahman R. , Rangan Bavana V. , Banerjee Subhash , Brilakis Emmanouil S. 
Published: September 2010

AbstractObjectives:To evaluate the intercatheter reproducibility of catheter‐based intracoronary near‐infrared spectroscopy (NIRS) for the detection of coronary lipid core plaques (LCPs).Background:The intercatheter in vivo reproducibility of coronary NIRS findings has not been evaluated.Methods:NIRS assessment using an automated pullback catheter was performed in triplicates in 10 patients using two different NIRS catheters to evaluate the reproducibility of the lipid core burden index (LCBI).Results:Mean age was 62 ± 7 years, and all patients were men with high prevalence of hypertension (90%), hyperlipidemia (90%), diabetes (50%), and current smoking (50%). The mean LCBI measured by the first and second catheters was 64 ± 55 and 69 ± 47, respectively (intraclass correlation coefficient, 0.97; 95% confidence interval, 0.90–0.99; and Spearman's ρ, 0.95; P < 0.001 for both). The mean LCBI measured again with the second catheter was 71 ± 63 (intraclass correlation coefficient, 0.96; 95% confidence interval, 0.84–0.99; and Spearman's ρ, 0.95; P < 0.001 for both). The mean number of LCPs detected by the first and second catheters was 1.9 ± 1.2 and 2.0 ± 1.5, respectively (intraclass correlation coefficient, 0.75, P = 0.005 and Spearman's ρ, 0.74, P = 0.01). The mean number of LCPs at reimaging with the second catheter was 2.0 ± 1.76 (intraclass correlation coefficient, 0.96 and Spearman's ρ, 0.98; P < 0.001 for both).Conclusion:NIRS measurements of LCP with two different catheters have high reproducibility. The findings support the use of NIRS to assess the status of LCP in patients over time and to assess LCP changes in response to novel treatments. © 2011 Wiley‐Liss, Inc.
Left atrial appendage closure with amplatzer cardiac plug in atrial fibrillation: Initial european experience
Authors: Park Jai‐Wun , Bethencourt Armando , Sievert Horst , Santoro Gennaro , Meier Bernhard , Walsh Kevin , Lopez‐Minquez Jose Ramon , Meerkin David , Valdés Mariano , Ormerod Oliver , Leithäuser Boris 
Published: September 2010

AbstractBackground: In most patients with atrial fibrillation (AF) and stroke, there is thrombotic embolization from the left atrial appendage (LAA). Percutaneous closure of the LAA is a novel alternative for the treatment of patients with AF at a high risk of stroke, in whom long‐term anticoagulation therapy is not possible or not desired. This study details the initial experience with the Amplatzer Cardiac Plug (ACP) in humans. Methods: Investigator‐initiated retrospective preregistry data collection to evaluate procedural feasibility and safety up to 24 hr after implantation of the ACP, a nitinol device designed for percutaneous trans‐septal implantation in LAA of patients with paroxysmal, permanent, or persistent AF. Results: In 137 of 143 patients, LAA occlusion was attempted, and successfully performed in 132 (96%). There were serious complications in 10 (7.0%) patients (three patients with ischemic stroke; two patients experienced device embolization, both percutaneously recaptured; and five patients with clinically significant pericardial effusions). Minor complications were insignificant pericardial effusions in four, transient myocardial ischemia in two, and loss of the implant in the venous system in one patient. Conclusion: The implantation of the ACP device is a feasible method for percutaneous occlusion of the LAA. © 2011 Wiley‐Liss, Inc.
Photosensitive Surfactants with Various Hydrophobic Tail Lengths for the Photocontrol of Genomic DNA Conformation with Improved Efficiency
Authors: Diguet Antoine , Mani Naresh Kumar , Geoffroy Marie , Sollogoub Matthieu , Baigl Damien 
Published: September 2010

AbstractWe report the synthesis and characterisation of photosensitive cationic surfactants with various hydrophobic tail lengths. These molecules, called AzoCx, are used as photosensitive nucleic acid binders (pNABs) and are applied to the photocontrol of DNA conformation. All these molecules induce DNA compaction in a photodependent way, originating in the photodependent polarity of their hydrophobic tails. We show that increasing hydrophobicity strongly enhances the compaction efficiencies of these molecules, but reduces the possibility of reversible photocontrol of a DNA conformation. Optimal performance was achieved with AzoC5, which allowed reversible control of DNA conformation with light at a concentration seven times smaller than previously reported.
ChemInform Abstract: Crystal Structure of CsHg5Cl11.
Authors: PAKHOMOV V. I. , GORYUNOV A. V. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Kinetics of the Oxidation of Chromium(II) by Hydrogen Peroxide. Effect of Different Anions, Temperature, and Pressure.
Authors: GAEDE W. , VAN ELDIK R. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Spectrokinetic Study of SF5 and SF5O2 Radicals and the Reaction of SF5O2 with NO.
Authors: SEHESTED J. , ELLERMANN T. , NIELSEN O. J. , WALLINGTON T. J. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Nuclear Magnetic Resonance, Force Field, and MNDO Studies on 1‐ Methoxycycloalkenes
Authors: TASKINEN E. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Intermolecular Interactions Responsible for the Absence of Chiral Recognition: Aromatic C‐H×××O Hydrogen Bonding in the Crystal Structure of 3‐Chloro‐9,13‐dibutylamino‐1‐hydroxypropyl‐6‐ trifluomethylphenanthrene Propan‐2‐ol Solvate Hydrochloride.
Authors: CAMILLERI P. , EGGLESTON D. S. , RZEPA H. S. , WEBB M. L. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Chiral Organometallic Reagents. Part 10. Configurational Stability of Chiral Organolithium Compounds on the Time Scale of Their Addition to Aldehydes.
Authors: HOFFMANN R. W. , JULIUS M. , CHEMLA F. , RUHLAND T. , FRENZEN G. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Convenient Procedures for the Asymmetric Reductions Utilizing α,. alpha.‐Diphenylpyrrolidinemethanol and Borane Complexes Generated Using the I2/NaBH4 System.
Authors: PERIASAMY M. , KANTH J. V. B. , PRASAD A. S. B. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Chiral Base‐Mediated Asymmetric Synthesis of Tricarbonyl(η6‐arene) chromium Complexes.
Authors: PRICE D. A. , SIMPKINS N. S. , MACLEOD A. M. , WATT A. P. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: 4,4′‐Di‐tert‐butylbiphenyl‐Catalyzed Reductive Opening of Azetidines with Lithium: A Direct Preparation of 3,N‐Dilithioalkylamines.
Authors: ALMENA J. , FOUBELO F. , YUS M. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Carbenes and the O‐H Bond: Norbornenylidenes.
Authors: KIRMSE W. , MEINERT T. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Scope and Mechanism of the Electron Transfer Photoinduced Alkylation of an Aromatic Nitrile.
Authors: FAGNONI M. , MELLA M. , ALBINI A. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Substituent Effects on Photochemical Hydrogen Abstraction in 2‐ Acylpyridines, 2‐Acylpyrazines, and 4‐Acylpyrimidines.
Authors: RAO C. J. , AGOSTA W. C. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Regioselectivity in Photochemical Chlorocarbonylation of Carbonyl Compounds.
Authors: BASHIR‐HASHEMI A. , HARDEE J. R. , GELBER N. , QI L. , AXENROD T. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Aromatic Iodination with the I2‐HgX2 Combination.
Authors: BACHKI A. , FOUBELO F. , YUS M. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Convenient Procedure for Preparing 2‐(3‐Iodopropyl)‐2‐methyl‐1,3‐ dioxolane (II).
Authors: WU Y. , AHLBERG P. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Silylation of 2‐ or 3‐Alkenenitriles with Iodosilanes/Triethylamine Affording 2‐ or 4‐Silyl‐2‐alkenenitriles.
Authors: YAMASHITA H. , REDDY N. P. , TANAKA M. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Transition Metal Catalyzed Substitution Reaction of Allylic Phosphates with Grignard Reagents.
Authors: YANAGISAWA A. , NOMURA N. , YAMAMOTO H. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Applications of the Baylis‐Hillman Reaction. Part 2. A Simple Stereoselective Synthesis of (E)‐ and (Z)‐Trisubstituted Alkenes.
Authors: BASAVAIAH D. , SARMA P. K. S. , BHAVANI A. K. D. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Catalytic Performance and Mechanism for Oxygenated Compound Formation for Ethylene Hydroformylation Over Supported Ru‐M Bimetallic Carbonyl Cluster‐Derived Catalysts.
Authors: XIAO F.‐S. , ICHIKAWA M. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Facile One‐Pot Thermal Dehydration and Dethioacetalization of β‐ Hydroxydithioacetals with Dimethyl Sulfoxide: Synthesis of α,. beta.‐Unsaturated Aldehydes.
Authors: RAO C. S. , CHANDRASEKHARAM M. , PATRO B. , ILA H. , JUNJAPPA H. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Anionic Cyclization of Olefinic Alkyllithiums: Ring Closure of Terminally Substituted 5‐Hexenyllithiums.
Authors: BAILEY W. F. , GAVASKAR K. V. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.