bluuebunny/crossref_metadata_2025_split huggingface dataset:
mRNA and protein levels of FUS, EWSR1, and TAF15 are upregulated in liposarcoma
Authors: Spitzer Jessica I. , Ugras Stacy , Runge Simon , Decarolis Penelope , Antonescu Christina , Tuschl Tom , Singer Samuel 
Published: February 2011

AbstractTranslocations or mutations of FUS, EWSR1, and TAF15 (FET) result in distinct genetic diseases. N‐terminal translocations of any FET protein to a series of transcription factors yields chimeric proteins that contribute to sarcomagenesis, whereas mutations in the conserved COOH‐terminal domain of wild‐type FUS were recently shown to cause familial amyotrophic lateral sclerosis. We thus investigated whether the loss of one FUS allele by translocation in liposarcoma may be followed by mutations in either the remaining FUS allele or the paralogous EWSR1. Furthermore, we investigated the strength of the FET promoters and their contributions to sarcomagenesis given the proteins' frequent involvement in oncogenic translocations. We sequenced the respective genomic regions of both FUS and EWSR1 in 96 liposarcoma samples. Additionally, we determined FET transcript and protein levels in several liposarcoma cell lines. We did not observe sequence variations in either FUS or EWSR1. However, protein copy numbers reached an impressive 0.9 and 5.5 Mio of FUS and EWSR1 per tumor cell, respectively. Compared with adipose‐derived stem cells, FUS and EWSR1 protein expression levels were elevated on average 28.6‐fold and 7.3‐fold, respectively. TAF15 mRNA levels were elevated on average 3.9‐fold, although with a larger variation between samples. Interestingly, elevated TAF15 mRNA levels did not translate to strongly elevated protein levels, consistent with its infrequent occurrence as translocation partner in tumors. These results suggest that the powerful promoters of FET genes are predominantly responsible for the oncogenic effect of transcription factor translocations in sarcomas. © 2011 Wiley‐Liss, Inc.
Clinical significance of genome‐wide minimally deleted regions in oral squamous cell carcinomas
Authors: Chien Huei‐Tzu , Liao Chun‐Ta , Huang Shiang‐Fu , Chen I‐How , Liu Tsung‐Yun , Jou Yuh‐Shan , Wang Hung‐Ming , Hsieh Ling‐Ling 
Published: February 2011

AbstractOral squamous cell carcinoma (OSCC) has the highest rate of increase among male cancers in Taiwan. An understanding of the molecular pathogenesis of this disease as well as the development of prognostic markers for the clinical management of this disease is very important. Thus, a systematic loss of heterozygosity (LOH) analysis was performed to define minimally deleted regions (MDRs) in 63 male OSCCs using 400 polymorphic microsatellite markers. For increasing reliability, genomic DNA was extracted from >90% tumor cells that had been purified by LCM, and only when a microsatellite marker provided LOH information in >30% of the OSCCs was there considered to be successful allelotyping. A correlation of the various MDRs with clinicopathological parameters and prognosis was carried out. In total, 32 MDRs were identified and ten were noted as novel. In addition, six MDRs were found to be associated with cigarette smoking. Among these markers, a loss of MDR c7r2 (7q32.2‐q35) was significantly associated with poor disease‐free survival (DFS) and ten MDRs were associated with allelic imbalance (AI) in tumors. Among the latter, a loss of MDR c14r1 (14q24.2‐q32.12) and c11r1 (11q13.4‐q25) had a synergistic effect on poor DFS and were able to reduce further the DFS rate in patients with MDR c7r2 loss. Taken together, the results generated in this study provide new insights that help with exploring the molecular mechanisms associated with OSCC tumorigenesis and cigarette smoking. They also should aid the development of potential prognostic markers for the clinical management of OSCC. © 2011 Wiley‐Liss, Inc.
Association between type‐three metabotropic glutamate receptor gene (GRM3) variants and symptom presentation in treatment refractory schizophrenia
Authors: Bishop Jeffrey R. , Miller Del D. , Ellingrod Vicki L. , Holman Timothy 
Published: February 2011

AbstractObjectivePositive associations between polymorphisms in the type‐three metabotropic glutamate receptor gene (GRM3) and the pathogenesis of schizophrenia as well as response to antipsychotic treatment have been reported. The objective of this study was to determine whether refractory psychiatric symptoms in antipsychotic non‐responders are related to polymorphisms in GRM3.MethodsNinety‐five treatment refractory schizophrenia participants were enrolled. Prior to a medication switch, global psychopathology and negative symptoms were rated. These participants were genotyped for seven markers in GRM3. Genotype associations with symptoms were assessed.ResultsTwo markers in GRM3 (rs1989796 and rs1476455), were associated with the presence of refractory global symptoms as measured by the Brief Psychiatric Rating Scale (BPRS) Total scores. Participants with an rs1476455_CC genotype had significantly higher BPRS scores than A‐carriers (55.1 ± 10.4 vs. 48.3 ± 9.2; F = 7.6, p = 0.0071). Additionally, participants with the rs1989796_CC genotype had significantly higher BPRS scores than T‐carriers (50.1 ± 5.7 vs. 55.8 ± 10.5, F = 7.1, p = 0.0091). No evidence for significant associations with negative symptoms was observed.ConclusionsPolymorphisms in the GRM3 gene may be associated with refractory global psychosis symptoms but not negative symptoms in persons with schizophrenia. Copyright © 2011 John Wiley & Sons, Ltd.
Predictors of 12‐week remission in a nationwide cohort of people with depressive disorders: the CRESCEND study
Authors: Kim Jae‐Min , Kim Sung‐Wan , Stewart Robert , Kim Seon‐Young , Yoon Jin‐Sang , Jung Sung‐Won , Lee Min‐Soo , Yim Hyeon‐Woo , Jun Tae‐Youn 
Published: February 2011

AbstractObjectiveTo estimate the 12‐week remission rate of patients with depressive disorders and predictors of this in a naturalistic clinical setting in South Korea.MethodsFor people with DSM‐IV depressive disorders about to receive treatment at 18 hospitals, data on sociodemographic and health status were obtained. A free choice of clinical interventions was allowed and naturalistic follow‐up took place at 1, 2, 4, 8, and 12 weeks later. Remission was defined as a Hamilton Depression Rating Scale score of ≤7 sustained to 12 weeks or last follow‐up, if earlier.ResultsFor 723 participants, the 12‐week remission rate was 31.4%. Remission was more likely in women, and in patients without a prior history of suicide attempt, and those with lower baseline anxiety.ConclusionsRemission associated with unrestricted clinical interventions was comparable to STAR*D estimates for citalopram alone. Comorbid anxiety and a previous suicide attempt were markers of worse outcome. Copyright © 2011 John Wiley & Sons, Ltd.
Characterization of major discontinuities from borehole cores of the black consolidated marl formation of Draix (French Alps)
Authors: Neuville A. , Toussaint R. , Schmittbuhl J. , Koehn D. , Schwarz J.‐O. 
Published: February 2011

AbstractA quantitative description of rock discontinuities present in subsurface cores that were drilled (down to 20 m) in the marls of the Laval and Moulin catchments near Draix (France) is presented. Three kinds of discontinuities are studied: those fully open, those open but filled with clay rich material and those sealed with calcite. With a laser profiler, the topography of facing sides of typical discontinuities was measured with a normal resolution of 1 µm. The probability distribution of the elevation of each surface are obtained and shown to be normal. Possible self‐affine scaling invariance of the topography were explored. The mineralogical content of an interface between the marl bulk and the embedded calcite vein is also investigated using X‐ray computed tomography. Implication of this study for water transport in such discontinuities is addressed in a companion paper in the same issue. Copyright © 2011 John Wiley & Sons, Ltd.
Fracture aperture reconstruction and determination of hydrological properties: a case study at Draix (French Alps)
Authors: Neuville A. , Toussaint R. , Schmittbuhl J. 
Published: February 2011

AbstractWe propose two techniques for fracture aperture reconstruction. The first one is a correlation technique that estimates the normal aperture or the tangential shift across a discontinuity whose sides present geometrical similarities. The only required material is a pair of appropriately controlled images of each side. Here, the images are maps of the corresponding side topography, obtained from laser profilometry. Assuming a purely normal opening, it is possible, from two corresponding sides of a given discontinuity in a core log, to infer the precise geometry of the in situ aperture. The second technique allows to retrieve the three‐dimensional geometry of a sealed discontinuity from non‐independent topography measurements of both sides. Both techniques are applied to discontinuities extracted from a core drilled down to 20 m in a fractured marl formation at Draix (French Alps). The probability density functions of the aperture of the sealed and open discontinuities are shown to be Gaussian. At the sample scale, the sealed fracture aperture is self‐affine, while the open one shows a cross‐over from a self‐affine regime at very small scales to an uncorrelated regime at largest scales. After extrapolating those scaling laws at the scale of the whole formation, we discuss when the aperture roughness affects the hydraulic properties of the Draix fractured bedrock. The overall estimated permeability is significant (10−9 − 10−8 m2), consistently with some previous indirect inferences. Copyright © 2011 John Wiley & Sons, Ltd.
Evaluation of the environmental impact of apple pest control strategies using pesticide risk indicators
Authors: Ioriatti Claudio , Agnello Arthur M , Martini Fabrizio , Kovach Joseph 
Published: February 2011

Abstract
Various pesticide risk indicators have been developed for estimating pesticide impact on human health and the environment. The present work applied a pesticide risk indicator to estimate change in pesticide risk in apple production between 2001 and 2009. The “Environmental Impact Quotient” was used, which evaluates potential impacts of pesticide active ingredients on farm workers, consumers, and nontarget organisms. A modified Environmental Impact Quotient was also tested, which accounts for all ingredients in the formulation presenting a health or environmental hazard, as identified in the Security Data Sheet. Irrespective of the rating system applied, an overall average improvement in environmental impact of apple protection strategies was indicated ranging from 23 to 24%. Hazard reduction was more significant when estimated per treatment, and was higher for acaricides and insecticides than for fungicides. Improvement appeared to be a consequence of using more selective and more effective active ingredients, applying alternative pest control techniques, compulsory periodic sprayer calibration, and wider use of dwarfing orchards. The modified Environmental Impact Quotient does not overcome all limitations regarding accuracy of pesticide risk indicators, but its ease of use in relying on official, easily accessible data, and the consistency of its results, makes it a good candidate for monitoring the success of reduced risk policies. Integr Environ Assess Manag 2011;7:542–549. © 2011 SETAC
Cut costs using working capital management
Authors: Sagner James S. 
Published: February 2011

AbstractManaging working capital involves organizing your company's short‐term resources to sustain ongoing activities, mobilize funds, and optimize liquidity. The two most critical cost efficiencies are float and processing expenses.The author gives some examples of firms using working capital management to control costs, and he includes a checklist of 20 actions companies should take to understand their working capital requirements—and produce cost savings. © 2011 Wiley Periodicals, Inc.
E‐discovery and its global implications
Authors: Fanning Kurt , Drogt Emily 
Published: February 2011

AbstractWe've all been following the saga of WikiLeaks and the electronic government documents it disclosed. And during congressional hearings on the financial crisis, lawmakers grilled Goldman Sachs executives over the content of their e‐mails, airing Goldman's “dirty laundry.” But your company's e‐mails and sensitive electronic documents can also be disclosed—in connection with some civil or criminal case. This process is called e‐discovery, and it can threaten the privacy of both US and multinational companies. What special challenges does this pose for companies? And what actions should your company take to cope? The authors of this article explain what you need to know. © 2011 Wiley Periodicals, Inc.
Capital investment decisions: Moving beyond traditional financial models
Authors: Boedeker Russell R. , Hughes Susan B. , Paulson Gjerde Kathy A. 
Published: February 2011

AbstractCompanies can supplement their traditional capital budgeting techniques with a strategic analysis that helps align both short‐ and long‐term goals. In fact, a strategic analysis of this kind may be the only technique applicable for decisions in which cash inflows are difficult to estimate. Because a strictly financial analysis may lead to different decisions than a strategic analysis, managers need to develop appropriate review criteria that can take into account both strategic and financial analyses, when appropriate. © 2011 Wiley Periodicals, Inc.
Basal and Src kinase‐mediated activation of the EphA2 promoter requires a cAMP‐responsive element but is CREB‐independent
Authors: Du Xiaojian , Baldwin Cindy , Hooker Erika , Glorion Pauline , Lemay Serge 
Published: February 2011

AbstractWe have previously identified the EphA2 receptor tyrosine kinase as a potentially important injury‐responsive gene and a transcriptional target of Src kinase activity in renal ischemia‐reperfusion injury (IRI). In the present study, we confirmed, using EphA2 gene trap mice that the endogenous EphA2 promoter is strongly activated following renal IRI. We also examined in more detail the mechanisms responsible for Src kinase‐induced activation of the −2 kb human EphA2 promoter and found that the minimal Src‐responsive elements were contained in the −145 to +137 region of the human EphA2 gene. This region contains a canonical cAMP‐responsive element (CRE) that we found to be critical for both basal and Src kinase‐induced transcriptional activity. However, despite activation of the prototypical CRE‐binding factor CREB by the Src kinase Fyn, siRNA‐mediated knockdown of CREB had no significant impact on either basal or Fyn‐induced EphA2 promoter activity. Similarly, activation of CREB by the adenylate cyclase agonist forskolin failed to induce EphA2 promoter activation. Thus, Src kinase‐induced activation of the EphA2 promoter is CRE‐dependent but CREB‐independent. J. Cell. Biochem. 112: 1268–1276, 2011. © 2011 Wiley‐Liss, Inc.
Novel catalytic bromolactonization of alkenoic acids using iodobenzene and Oxone®
Authors: He Yan , Pu Ye , Shao Bo , Yan Jie 
Published: February 2011

AbstractThe novel catalytic reaction for bromolactonization of alkenoic acids is reported. When iodobenzene is used as recyclable catalyst in combination with Oxone® as terminal oxidant, the cyclization of various 4‐pentenoic acids with sodium bromide is easily carried out in CF3CH2OH at room temperature and giving five‐membered bromolactones in good yields. J. Heterocyclic Chem., 2011.
Synthesis of novel quinoline analogues of nimesulide: An unusual observation
Authors: Reddy Lingam Venkata , Nakka Mamatha , Suman Alishetty , Ghosh Soumen , Helliwell Madeleine , Mukkanti Khagga , Mukherjee Alok Kumar , Pal Sarbani 
Published: February 2011

AbstractReduction of nimesulide followed by treating the N‐acyl derivative of resulting arylamine with Vilsmeier‐Haack reagent provided novel 2‐chloro‐3‐formylquinoline derivatives. The construction of quinoline ring using Vilsmeier‐Haack reagent afforded an unexpected compound, N‐(2‐chloro‐3‐formyl‐7‐phenoxy quinolin‐6‐yl)formamide, in addition to the expected product. The structure of this unexpected quinoline derivative was established via single‐crystal X‐ray analysis and its formation could be explained by an unprecedented N‐S bond cleavage under Vilsmeier‐Haack reaction conditions. The 2‐chloro‐3‐formylquinoline derivatives obtained were converted to a number of corresponding Schiff bases with potential pharmacological importance. J. Heterocyclic Chem., 2011.
Effect of low‐magnitude, high‐frequency vibration on osteogenic differentiation of rat mesenchymal stromal cells
Authors: Lau Esther , Lee W. David , Li Jason , Xiao Andrew , Davies John E. , Wu Qianhong , Wang Liyun , You Lidan 
Published: February 2011

AbstractWhole body vibration (WBV), consisting of a low‐magnitude, high‐frequency (LMHF) signal, is anabolic to bone in vivo and may act through alteration of the lineage commitment of mesenchymal stromal cells (MSC). We investigated the effect of LMHF vibration on rat bone marrow‐derived MSCs (rMSCs) in an in vitro system. We subjected rMSCs to repeated (six) bouts of 1‐h vibration at 0.3g and 60 Hz in the presence of osteogenic (OS) induction medium. The OS differentiation of rMSCs under the loaded and non‐loaded conditions was assessed by examining cell proliferation, alkaline phosphatase (ALP) activity, mRNA expression of various osteoblast‐associated markers [ALP, Runx2, osterix (Osx), collagen type I alpha 1 (COL1A1), bone sialoprotein (BSP), osteopontin (OPN), and osteocalcin (OCN)], and matrix mineralization. LMHF vibration did not enhance the OS differentiation of rMSCs. Surprisingly, the mRNA level of Osx, a transcription factor necessary for osteoblast formation, was decreased, and matrix mineralization was inhibited. Our findings suggest that LMHF vibration may exert its anabolic effects in vivo via mechanosensing of a cell type different from MSCs. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 29: 1075–1080, 2011
Do mucosal folds in the eustachian tube function as microturbinates?
Authors: Ozturk Kayhan , Snyderman Carl H. , Sando Isamu 
Published: February 2011

AbstractObjectives/Hypothesis:Mucosal folds in the eustachian tube (ET) may function as microturbinates and provide protection and clearance.Study Design:Descriptive study.Methods:Nineteen whole‐mount temporal bone ET specimens (nine pediatric and 10 adult) were evaluated in the present study. Five specific localizations for each case were selected. The cartilaginous segment of the ET was divided vertically and the length of mucosal surfaces and the number of mucosal folds of anterior and posterior walls on the histological sections were analyzed with MetaMorph 7.5.2.0 software.Results:The length of the mucosal surface of the posterior wall of the ET was longer than the anterior wall (P < .05). In pediatric specimens, the posterior wall had more mucosal folds than adult specimens (P < .05).Conclusions:Microturbinates in the posterior wall of the ET may provide important protection and clearance functions for children and play a role in the pathogenesis of eustachian tube dysfunction. Laryngoscope, 2011
A Case of preoperative “first‐bite syndrome” associated with mucoepidermoid carcinoma of the parotid gland
Authors: Diercks Gillian R. , Rosow David E. , Prasad Mukesh , Kuhel William I. 
Published: February 2011

AbstractFirst‐bite syndrome, characterized by excruciating pain in the parotid region after the first few bites of food, can be seen after surgery of the parapharyngeal space. Herein we describe a patient with no prior surgical history who presented with facial pain consistent with first‐bite syndrome. Imaging and ultrasound‐guided FNA revealed a nonpalpable mucoepidermoid carcinoma of the parotid gland, which was removed surgically with facial nerve preservation. After surgical intervention, the patient's symptoms resolved. An extensive literature review demonstrated that this is the first description of a preoperative first‐bite syndrome, or first‐bite syndrome associated with the presence a parotid mass. Laryngoscope, 2011
Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate‐Leached, Cross‐Linked Poly(ε‐caprolactone) Scaffold
Authors: Baker Simon C. , Rohman Geraldine , Hinley Jennifer , Stahlschmidt Jens , Cameron Neil R. , Southgate Jennifer 
Published: February 2011

AbstractFlexible, strong scaffolds were created by crosslinking PCL with 1,6‐hexamethylenediisocyanate, using paraffin beads as a porogen. Particulate leaching generated homogeneous scaffolds with interconnected spherical pores of 5–200 µm. Subcutaneous implantation in rats for 3 months resulted in minimal scaffold resorption and a non‐inflammatory regenerative host response, with complete infiltration by alternatively‐activated CD68+ macrophages. In addition, scaffolds were populated extensively along microfractures by a stromal matrix, which was highly vascularised and contained a subset of stromal cells that expressed the anti‐inflammatory CD163 antigen. Such microfractures may be an important physical feature for directing stromal integration and vascularisation events. magnified image
Synthetic analogues of biogenic magnetite: synthesis and characterization of magnetite nanoparticles
Authors: Dudchenko N. O. 
Published: February 2011

AbstractBiogenic magnetite serve for a wide range of biological functions, including the orientation of animals in the space and also play an important role in the brain functioning. We describe the synthesis and characterization of magnetite nanoparticles that are the synthetic analogues of biogenic magnetite. Magnetite nanoparticles were prepared via co‐precipitation of iron (II) and iron (III) with sodium hydroxide in aqueous solution at high temperature (80°C). To avoid the oxidation of the magnetite surface, further covering of the magnetite nanoparticles with (3‐aminopropyl)triethoxysilane was performed. The obtained magnetite nanoparticles had a comparatively high magnetization (45 A·m2/kg) and crystallinity. The average diameter of synthesized magnetite nanoparticles was around 14 nm. We could conclude that the characteristics of the synthesized magnetite nanoparticles (size, phase composition, magnetic properties) are similar to biogenic magnetite.
Order reduction in computational inelasticity: Why it happens and how to overcome it—The ODE‐case of viscoelasticity
Authors: Eidel Bernhard , Kuhn Charlotte 
Published: February 2011

AbstractTime integration is the numerical kernel of inelastic finite element calculations, which largely determines their accuracy and efficiency. If higher order Runge–Kutta (RK) methods, p≥3, are used for integration in a standard manner, they do not achieve full convergence order but fall back to second‐order convergence. This deficiency called order reduction is a longstanding problem in computational inelasticity. We analyze it for viscoelasticity, where the evolution equations follow ordinary differential equations. We focus on RK methods of third order. We prove that the reason for order reduction is the (standard) linear interpolation of strain to construct data at the RK‐stages within the considered time interval. We prove that quadratic interpolation of strain based on tn, tn + 1 and, additionally, tn − 1 data implies consistency order three for total strain, viscoelastic strain and stress. Simulations applying the novel interpolation technique are in perfect agreement with the theoretical predictions. The present methodology is advantageous, since it preserves the common, staggered structure of finite element codes for inelastic stress calculation. Furthermore, it is easy to implement, the overhead of additional history data is small and the computation time to obtain a defined accuracy is considerably reduced compared with backward Euler. Copyright © 2011 John Wiley & Sons, Ltd.