bluuebunny/crossref_metadata_2025_split huggingface dataset:
Addressing the challenges of acute toxicity hazard classification using a non-animal defined approach
Authors: Macmillan Donna S , Ambure Pravin , Aranda Vicente , Bayona Yannick , Bonderovic Vera , Dawick James , Fabre Nicolas , Fischer Stephan , Hodges Geoff , Llobet-Mut Ágata , Loisel-Joubert Sophie , Rivetti Claudia , Roberts Jayne , Schirmer Kristin , Serrano-Candelas Eva , Serrano Ramón Blanca , Stackhouse Ricky A 
Published: February 2025

Abstract
Acute fish toxicity is an ecotoxicological endpoint which provides important information about a chemical’s potential to elicit adverse effect(s) on fish. These effects are typically studied using in vivo tests but for animal welfare reasons as well as the quest for increased species relevance, biological coverage, mechanistic understanding of effects and throughput, there have been significant efforts in recent years to reduce or replace the use of animals in (eco)toxicological hazard assessment, by developing defined approaches (DA) or integrated approaches to testing and assessment (IATA). To this end, a novel score-based DA has been developed, which integrates three in silico predictions from freely available (Q)SARs: the VEGA Fish (KNN-Read-Across) and Fathead Minnow (KNN-IRFMN) models and the United States Environmental Protection Agency (USEPA) ECOSAR Fish 96-h LC50 model, along with in vitro RTgill-W1 data. The DA provides a categorical output aligned with the United Nations Globally Harmonized System of Classification and Labelling (GHS) framework (Acute Category 1, Acute Category 2, Acute Category 3 or Not Classified) with an overall accuracy of 80%, offering a reliable alternative to traditional in vivo testing methods for acute fish toxicity.
May Measurement Month 2022: an analysis of blood pressure screening results from South Africa
Authors: Woodiwiss Angela J , Orchard Ane , Kolkenbeck-Ruh Andrea , Ware Lisa J , Mels Catharina M C , Yates Samantha , Prakaschandra Rosaley , Jones Erika S W , Pillay Sarusha , Beaney Thomas , Kerr Gabriele , Poulter Neil R 
Published: February 2025

Abstract
The May Measurement Month (MMM) campaign was carried out in South Africa in 2022 with the aim of raising awareness of raised blood pressure (BP). Here, we report on the findings of the campaign.
Adults aged ≥ 18 years were recruited opportunistically at university campuses, community centres, schools, sport complexes, public parks, shopping centres, hospitals, and through household visits. Three seated BP readings were taken for each participant, along with completion of a questionnaire on demographics, lifestyle factors, and comorbidities. Hypertension was defined as a systolic BP ≥ 140 mmHg and/or diastolic BP ≥ 90 mmHg or being on antihypertensive medication. Controlled BP was defined as being on antihypertensive medication with a BP &lt;140/90 mmHg. Multiple imputation was used to estimate any missing BP readings. In total, 4602 were screened, with a mean age of 31.9 years and 61.9% of whom were female. Of all participants, 1217 (26.5%) had hypertension, of whom 456 (37.5%) were aware, and 406 (33.4%) were on antihypertensive medication. Of those on antihypertensive medication, 205 (50.5%) had controlled BP, and of all participants with hypertension, 16.8% had controlled BP.
The MMM campaign in South Africa identified significant numbers of participants with either untreated or inadequately treated hypertension. The high prevalence of hypertension, despite the young adult age, together with the high proportions of individuals unaware of their hypertension and with uncontrolled BP highlight the need for hypertension screening and awareness campaigns, and more rigorous management of hypertension.
Comprehensive mapping of individual living and dead tree species using leaf-on and leaf-off ALS and CIR data in a complex temperate forest
Authors: Lisiewicz Maciej , Kamińska Agnieszka , Kraszewski Bartłomiej , Kuberski Łukasz , Pilch Kamil , Stereńczak Krzysztof 
Published: February 2025

Abstract
Tree species information is crucial both for understanding forest composition and supporting sustainable forest management, but also for monitoring biodiversity and assessing ecosystem services. Remote sensing data has been widely used to map the spatial distribution of tree species across large areas. However, there is currently a lack of studies demonstrating the potential of airborne laser scanning data collected during different seasons to identify multiple individual tree species/genera, including dead individuals. The main objective of this study was to map the ecologically valuable forest area constituting the Polish part of the Białowieża Forest using leaf-on and leaf-off airborne laser scanning (ALS) data and color-infrared imagery. Eleven living species/genera (alder, ash, aspen, birch, hornbeam, lime, maple, oak, pine, spruce and other deciduous) and four dead classes (dead deciduous, dead pine, dead spruce and snag) were classified at the individual tree level. Applying the Random Forests algorithm and a set of 30 predictor variables, 15 classes were classified with an overall accuracy of 82 per cent. The mapping of nearly 20 million individual trees revealed that in 2015, the most common tree species in the upper part of the Białowieża Forest stands was spruce (20.1 per cent), followed by alder (19.0 per cent) and pine (18.1 per cent). Among dead trees, dead deciduous trees (2.2 per cent) and dead spruce (1.7 per cent) were the most common. Our results can serve as a first cornerstone for carrying out further in-depth analyses of forest biodiversity using remote sensing data in this exceptional forest area.
Unpacking family meals: state-of-the-art review critiquing the Western ideals, positioning and promotion of family meals
Authors: Le Moal Fairley , Litterbach Eloise-kate , Dunn Katherine , Fraser Kylie , Bouchaud Celeste C , Middleton Georgia 
Published: February 2025

Abstract
Family meals are a familiar concept and are practised in many Western households. While academics have been researching family meals for decades, there is much about the family meal we still do not understand. Meanwhile, the promotion of an ‘ideal’ of family meals across media, health and social discourse ensues. An extensive pool of research has indicated cross-sectional associations between the frequency of family meals and optimal health outcomes. However, evidence surrounding what specifically it is about the family meal that could offer health benefits is limited. Furthermore, family meals carry a level of moral value, evoking pressure for parents to ‘achieve’ a family meal ideal, unattainable for many. Family meals research has traditionally focussed on mothers’ perceptions and roles in family mealtimes. While literature is starting to emerge on the often-overlooked cognitive contribution required to plan and execute family meals, little structural supports exist to streamline these efforts or make them more achievable for contemporary families. The published literature is only starting to include cultural and demographic diversity, making it difficult to understand and promote realistic and feasible family meals across populations. The popular depiction of family meals needs to evolve alongside changes in social norms. Our call to action to address these issues is to draw on existing family meal practices, continue to diversify our investigations, and broaden our definitions and understandings of what a family meal is or could be, and how it should be measured.
From statistical physics to social sciences: the pitfalls of multi-disciplinarity
Authors: Bouchaud Jean-Philippe 
Published: February 2025

Abstract
This is an adapted version of my inaugural lecture at Collège de France in 2021. I reflect on the difficulty of multi-disciplinary research, which often hinges on unexpected epistemological and methodological differences, for example, about the scientific status of models. What is the purpose of a model? What are we ultimately trying to establish: rigorous theorems or ad hoc calculation recipes; absolute truth, or heuristic representations of the world? I argue that the main contribution of statistical physics to social and economic sciences is to make us realize that unexpected behavior can emerge at the aggregate level, which isolated individuals would never experience. Not only crises, panics, opinion reversals, the spread of rumors or beliefs, fashion effects, and the zeitgeist but also the existence of money, lasting institutions, social norms, and stable societies must be understood in terms of collective belief and/or trust, self-sustained by interactions, or on the contrary, the rapid collapse of this belief or trust. Agent-based models provide a powerful and natural framework to account for such phenomena.
A review of anthropogenic hazardous particulate matters: general description and adsorbent materials
Authors: Yang Jiaren , Shi Lin , Shi Fengcheng , Kuang Ying , Qian Hong , Wang Lejun , Zhang Ting , Jiang Fatang 
Published: February 2025

Abstract
Anthropogenic hazardous particulate matters (AHPM) refers to small airborne particles generated by human activities. This review aims to summarize the basic information on the classification, source, and distribution pattern of AHPM and its hazards. The classification of different adsorbent materials is summarized. Based on the characteristics of various adsorbent materials, the trend of new adsorbent materials in the future development is proposed. This provides researchers with new ideas for the study of adsorption of AHPM and gives an outlook on the research of new adsorbent materials and describes their potential application value.
SARS-COV-2-DRIVEN EPIDERMAL GROWTH FACTOR RECEPTOR SIGNALING AND THE COVID19-ASSCOCIATED PSYCHOSIS/SCHIZOPHRENIA
Authors: Nawa *Hiroyuki 
Published: February 2025

Abstract

Background
COVID-19 presents a wide range of symptoms, including respiratory issues and, intriguingly, neurological and psychiatric manifestations such as delirium and psychosis as well. Pathological studies reveal that the cytokine storm, particularly involving the activation of the epidermal growth factor receptor (EGFR), plays a crucial role in viral replication and the development of lung fibrosis. Notably, the spike domain of SARS-CoV-2 mimics EGF and directly triggers EGFR activation before inflammation occurs, suggesting new therapeutic options involving anti-EGFR kinase inhibitors to combat COVID-19 fibrosis. However, this finding regarding the biological activity of the spike protein also raises concerns about current and future psychiatric disorders associated with COVID-19.

Method
Recombinant EGF or SARS-CoV-2 spike protein is subcutaneously administered to neonatal mice, hamsters, and rats during their postnatal period. After 2-3 months, these animals undergo neurobehavioral tests and physiological recordings with institutional authorization.

Results
We are exploring the connections between EGFR signaling and schizophrenia, revealing significant molecular associations of the EGFR system in the blood and brain tissue of patients. Animal models exposed to EGF or the other EGFR ligands during perinatal stages exhibit various behavioral endophenotypes relevant to schizophrenia, including abnormalities in ASSR, d/fMMN, P300, ABR, and frontotemporal connectivity. The animal models also exhibit behavioral changes, especially an increase in dopaminergic activity post-puberty and neuronal hyperactivity in the auditory cortex, correlating with the onset of their behavioral impairments.

Discussion & Conclusion
This comprehensive discussion with the EGF animal model delves into the neuropsychiatric implications of prognostic symptoms of COVID-19 and their interaction with the EGFR system. However, the severity and prevalence of neurological/ psychiatric symptoms vary among COVID- 19 patients, and not all individuals with the virus experience CNS involvement. Further studies will help clarify the role of EGFR signaling in psychiatric symptoms and consequences in the context of COVID-19.
THE EFFECT OF DOPAMINE RECEPTOR D1 (DRD1) GENE VARIANTS ON CHANGES IN CRAVING SEVERITY DURING ALCOHOL WITHDRAWAL
Authors: Lin *Ze-yu , Huang Ming-Chyi , Lee Pin-Wei , Liu Tung-Hsia , Liu Yu-Li 
Published: February 2025

Abstract

Background
Alcohol dependence (AD) is a complex disorder with unclear underlying etiologies. One strategy to understand its pathophysiology involves investigating the behaviors or symptoms, namely subphenotypes, associated with AD. Given that aggression is a prevalent behavior in individuals with AD and is associated with a poor outcome, we attempted to examine the biological underpinnings of aggression as a subphenotype of AD in terms of genetic predisposition. Furthermore, we also explored the impact of genes on craving, a hallmark of AD and AD relapse.

Aims & Objectives
This study aimed to identify the association of aggression-related candidate genes with AD. In addition, we also investigated the genetic association with changes in craving severity following 2 weeks of alcohol withdrawal.

Method
A total of 210 treatment-seeking patients with AD and 117 healthy controls were recruited in this study. 7 candidate genes were screened based on genome-wide genotyping Axiom Genome-Wide TWB 2.0 Array, included MECOM, AVPR1A, DRD1, DRD2, DRD3, RBOX1, and HTR2A. We followed the Alcohol Craving Questionnaire-Short Form-Revised (ACQ-SF-R) and Obsessive-Compulsive Drinking Scale (OCDS), both of which were used to assess the severity of alcohol craving during withdrawal.

Results
Among the candidate genes, we identified a significant association of Dopamine Receptor D1 ( DRD1) gene with AD. The DRD1 single nucleotide polymorphisms (SNPs) (rs12518222, rs144667196, rs4867798, rs686, rs4532, rs5326, and rs265981) were further examined, and we found these SNPs were significantly associated with AD. The minor alleles of rs12518222, rs4867798, rs686, rs4532, rs5326, rs265981 were associated with higher craving scores throughout the withdrawal period.
Haplotype-based analyses also demonstrated a significant association of DRD1 gene with less craving alleviation during alcohol withdrawal.

Discussion & Conclusion
Our data indicates an association between the aggression-related gene DRD1 and AD within Taiwanese population. Particularly, patients harboring the minor alleles of DRD1 SNPs have a higher risk of AD and display a higher post-withdrawal craving scores compared to those harboring their counterparts. These findings, indicating that DRD1 gene plays a role in the susceptibility to AD and retards craving amelioration during the recovery phase, suggest that aggression might intricately involve neurobiological mechanisms contributing to AD.
SUPPLEMENTING WITH DEXTROMETHORPHAN MAY ENHANCE COGNITIVE FUNCTIONS IN YOUNG INDIVIDUALS WITH BIPOLAR DISORDERS
Authors: Huang *Chih-Chun , Chang Yun-Hsuan , Lu Ru-Band , Hong Jau-Shyong 
Published: February 2025

Abstract

Background
Bipolar disorder (BD) is a chronic, debilitating mental condition with consistent prevalence across genders and ethnicities. It's marked by high relapse rates, mood swings, and disruptions in various life aspects. Prior research has highlighted neuropsychological impairments in BD.

Aims & Objectives
This study investigates the therapeutic effect of dextromethorphan (DM), an open- label drug, and its influence on different age groups' neuropsychological functions in BD patients.

Methods
We recruited 92 BD patients, with 55.1% males. In a 12-week, randomized, double-blind study, we assigned patients to one of three groups: 29 on valproic acid (VPA) plus either placebo (VPA+P) or 63 with DM (30 mg per day or 60mg per day). We evaluated symptom severity using the Young Mania Rating Scale (YMRS) and Hamilton Depression Rating Scale (HDRS). Patients completed neuropsychological tasks at baseline and after the 12-week trial: the Wechsler Memory Scale (WMS) and the Continuous Performance Test (CPT). We also recorded clinical characteristics.

Results
Patients showed overall improvement in all neuropsychological functions after the 12-week trial. Subgrouping patients by age, the younger group (YG, younger than 39 years old) responded more positively to DM treatment, while the middle-old group (MOG, older than 40 years) exhibited less pronounced effects.

Discussion & Conclusion
This study provides valuable insights into DM's therapeutic effect, aligning with previous research. Age played a role in the therapeutic response, particularly among younger BD patients. Early treatment may yield better neuropsychological outcomes. To comprehensively capture neuroplasticity and therapeutic impact, especially on memory indices of WMS-III, we recommend future longitudinal studies. These would shed light on the long-term impact of age on BD patients' multifaceted functions.
CHANGES IN CORE DEPRESSIVE SYMPTOMS OVER TIME IN PERIPARTUM WOMEN: A NETWORK ANALYSIS
Authors: Zhang *Yuqun , Gao Ju , Qin Meixia , Zhao Weiying , Ding Yi , Chen Ying , Qiao Xuebin , Yue Xin , Yuan Yonggui 
Published: February 2025

Abstract

Background
Perinatal depression (PND) is common in pregnant and postpartum women. Previous research indicates that there are clinical subtypes of PND, and the time of onset is an important factor to identify PND subtypes. However, differences of PND in different trimesters and in the postpartum period are not clear. And studies examining core symptoms that could be used to identify PND subtypes are lacking.

Methods
Women during the second (N = 161) and third trimesters (N = 248) and within 6 weeks postpartum (N = 110) were recruited. All participants completed the assessment of the Edinburgh Postnatal Depression Scale. Network analysis was used to explore the relationships between depressive symptoms in all three periods.

Results
Postpartum women showed the highest prevalence of PND and the most severe depressive symptoms. The symptom ‘Sadness and misery’ had the strongest centrality in both the second and third trimesters, but decreased in the postpartum period. The symptom ‘Fear and panic’ had the strongest centrality in the postpartum period. All three PND networks were relatively stable in strength and closeness.

Conclusions
This study indicated the changes over time in central symptoms in pregnant and postpartum women, which shifted from being dominated by depression to being dominated by anxiety. Our findings suggested that more symptom-specific interventions should be used to improve the mental health of mothers, fetuses and infants. All of this provided clinical evidence to investigate the biological mechanisms of PND subtypes to help diagnose and treat PND with precision.
GHRELIN SIGNALLING MEDIATES SEX DIFFERENCES IN MIDBRAIN CONTROL OF BINGE DRINKING
Authors: Walker *Leigh C , Pearl Amy , Pinares-Garcia Paulo , Shesham Arnav , Maddern Xavier , Anversa Roberta G , Reed Felicia , Giardino William J , Lawrence Andrew J 
Published: February 2025

Abstract

Background
The circuitry mediating excessive alcohol consumption includes the understudied central projecting Edinger-Westphal (EWcp); a structure dense in neuropeptide expression, including cocaine and amphetamine regulated transcript (CART). While studies have shown a critical role for this nucleus in alcohol consumption, few studies have interrogated the contributions of distinct EWcp populations.

Aims
We aimed to understand the contribution of peptidergic EWcp cells in alcohol consumption, and hypothesized sex differences may be present.

Methods
To examine a functional role of these cells in binge drinking we used chemogenetics to inhibit EWcppeptidergic cells in male and female CART-Cre mice. We next used RNAscope to examined the neurochemistry of EWcppeptidergic cells observing strong overlap with the ghrelin receptor (GHSR) and using whole cell patch clamp electrophysiology, we assessed the response of EW peptidergic cells to bath application of ghrelin in male and female mice. Next, we knocked down Ghsr expression non-specifically in the EWcp of male and female mice using a shRNA and specifically from peptidergic (CART-Cre) and glutamatergic (vGlut2-Cre) populations using Cre-dependent Ghsr-shRNA. Finally, given ghrelin has high constitutive activity, we assessed whether a ghrelin antagonist (JMV2959) or inverse agonist (LEAP2) could reduce binge drinking when administered directly within the EW nucleus.

Results
Chemogenetic inhibition of EWcppeptidergic cells had no effect on binge drinking, anxiety behaviour or other consummatory behaviours in male mice; however, a specific reduction in alcohol binge drinking was observed in female mice. Ghsr mRNA was strongly expressed in EWcppeptidergic, and to a lesser extent in EWcpglutamatergic cells. Further, bath application of ghrelin increased firing frequency in EWcppeptidergic cells in both male and female mice, however, the change in response was significantly higher in females. Systemic ghrelin administration increased binge drinking in female mice, which was reduced by chemogenetic inhibition of EWcppeptidergic cells. In addition, non-specific EWcp Ghsr-shRNA knockdown reduced binge drinking specifically in female, but not male mice compared to scram-ShRNA controls.
Further in female mice, Ghsr-shRNA localised to EWcppeptidergic, but not EWcpvGlut2 cells reduced binge drinking. Finally, infusion of both a GHSR antagonist and inverse agonist directly within the EWcp reduced binge drinking in female mice.

Conclusions
Together, our results suggest the EWcp is a region mediating excessive alcohol bingeing through GHSR actions on peptidergic cells in female mice. Given the recent interest in GHSR1a inverse agonists for the treatment of alcohol use disorder, understanding the neural mechanism(s) underpinning how the ghrelin system mediates alcohol consumption are critical.
NPAS4 ENHANCER RNA FORMS DNA:RNA HYBRID R-LOOP TO CONTROL REWARD-RELATED BEHAVIORAL ADAPTATION THROUGH ACTIVITY-DEPENDENT GENE EXPRESSION
Authors: Akiki Rose Marie , Magami Kosuke , Cornbrooks Rebecca G , Greige Alain , Snyder Kirsten K , Wood Daniel J , Herrington Claire M , Mace Philip , Blidy Kyle , Koike Nobuya , Berto Stefano , Cowan Christopher W , Taniguchi *Makoto 
Published: February 2025

Abstract
Neuronal activation induces immediate early response genes expression, essential for behavioral adaptation to emotional stimuli. Npas4 is rapidly induced by synaptic activity, regulating behavioral responses to cocaine reward conditioning and chronic stress. A novel long non-coding RNA (LncRNA) transcribed from Npas4 enhancer is identified as essential for emotional stimuli-induced Npas4 mRNA expression and formation of cocaine reward-associated context in NAc and chronic stress-induced deficits in sucrose preference in mPFC. Notably, LncRNA forms DNA:RNA hybrid structure R-loop, required for synaptic activity-dependent Npas4 mRNA expression through regulating 3D chromatin loop structure connected between Npas4 promoter and enhancer. The findings highlight an unprecedented mechanism of LncRNA function through the formation of R-loop for 3D chromatin loop structure, providing novel insights into the regulation of synaptic activity-dependent gene expression.