bluuebunny/crossref_metadata_2025_split huggingface dataset:
Absence of IFN-β Impairs Antigen Presentation Capacity of Splenic Dendritic Cells via Down-Regulation of Heat Shock Protein 70
Authors: Ziętara Natalia , Łyszkiewicz Marcin , Gekara Nelson , Puchałka Jacek , Dos Santos Vitor A. P. Martins , Hunt Clayton R. , Pandita Tej K. , Lienenklaus Stefan , Weiss Siegfried 
Published: July 2009

AbstractType I IFNs play a key role in linking the innate and adaptive arms of the immune system. Although produced rapidly in response to pathogens, IFNs are also produced at low levels in the absence of infection. In the present study, we demonstrate that constitutively produced IFNs are necessary in vivo to maintain dendritic cells in an “Ag presentation-competent” state. Conventional dendritic cells (cDCs) isolated from spleens of IFN-β or IFNAR-deficient mice exhibit a highly impaired ability to present Ag and activate naive T cells. Microarray analysis of mRNA isolated from IFN-β−/− and IFNAR−/− cDCs revealed diminished expression of two genes that encoded members of the heat shock protein 70 (Hsp70) family. Consistent with this observation, pharmacological inhibition of Hsp70 in cDCs from wild-type mice impaired their T cell stimulatory capacity. Similarly, the Ag presentation ability of splenic cDCs isolated from Hsp70.1/3−/− mice was also severely impaired in comparison to wild-type cDCs. Thus, constitutive IFN-β expression regulates Hsp70 levels to help maintain dendritic cells in a competent state for efficient priming of effector T cells in vivo.
Digital Health Interventions and Patient Safety in Abdominal Surgery
Authors: Grygorian Artem , Montano Diego , Shojaa Mahdieh , Ferencak Maximilian , Schmitz Norbert 
Published: April 2024

ImportanceOver the past 2 decades, several digital technology applications have been used to improve clinical outcomes after abdominal surgery. The extent to which these telemedicine interventions are associated with improved patient safety outcomes has not been assessed in systematic and meta-analytic reviews.ObjectiveTo estimate the implications of telemedicine interventions for complication and readmission rates in a population of patients with abdominal surgery.Data SourcesPubMed, Cochrane Library, and Web of Science databases were queried to identify relevant randomized clinical trials (RCTs) and nonrandomized studies published from inception through February 2023 that compared perioperative telemedicine interventions with conventional care and reported at least 1 patient safety outcome.Study SelectionTwo reviewers independently screened the titles and abstracts to exclude irrelevant studies as well as assessed the full-text articles for eligibility. After exclusions, 11 RCTs and 8 cohort studies were included in the systematic review and meta-analysis and 7 were included in the narrative review.Data Extraction and SynthesisData were extracted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) reporting guideline and assessed for risk of bias by 2 reviewers. Meta-analytic estimates were obtained in random-effects models.Main Outcomes and MeasuresNumber of complications, emergency department (ED) visits, and readmissions.ResultsA total of 19 studies (11 RCTs and 8 cohort studies) with 10 536 patients were included. The pooled risk ratio (RR) estimates associated with ED visits (RR, 0.78; 95% CI, 0.65-0.94) and readmissions (RR, 0.67; 95% CI, 0.58-0.78) favored the telemedicine group. There was no significant difference in the risk of complications between patients in the telemedicine and conventional care groups (RR, 1.05; 95% CI, 0.77-1.43).Conclusions and RelevanceFindings of this systematic review and meta-analysis suggest that perioperative telehealth interventions are associated with reduced risk of readmissions and ED visits after abdominal surgery. However, the mechanisms of action for specific types of abdominal surgery are still largely unknown and warrant further research.
Plastic Wound Protector vs Surgical Gauze for Surgical Site Infection Reduction in Open GI Surgery
Authors: Yoo Nina , Mun Ji Yeon , Kye Bong-Hyeon , Kim Chang Woo , Lee Jae Im , Park Youn Young , Kang Byung Mo , Park Byung Kwan , Kwak Han Deok , Kang Won-Kyung , Bae Sung Uk , Oh Heung-Kwon , Hong Youngki , Kim Hyung Jin 
Published: April 2024

ImportanceSurgical site infections (SSIs) are prevalent hospital-acquired infections with significant patient impacts and global health care burdens. The World Health Organization recommends using wound protector devices in abdominal surgery as a preventive measure to lower the risk of SSIs despite limited evidence.ObjectiveTo examine the efficacy of a dual-ring, plastic wound protector in lowering the SSI rate in open gastrointestinal (GI) surgery irrespective of intra-abdominal contamination levels.Design, Setting, and ParticipantsThis multicenter, patient-blinded, parallel-arm randomized clinical trial was conducted from August 2017 to October 2022 at 13 hospitals in an academic setting. Patients undergoing open abdominal bowel surgery (eg, for bowel perforation) were eligible for inclusion.InterventionPatients were randomized 1:1 to a dual-ring, plastic wound protector to protect the incision site of the abdominal wall (experimental group) or a conventional surgical gauze (control group).Main Outcomes and MeasuresThe primary end point was the rate of SSI within 30 days of open GI surgery.ResultsA total of 458 patients were randomized; after 1 was excluded from the control group, 457 were included in the intention-to-treat analysis (mean [SD] age, 58.4 [12.1] years; 256 [56.0%] male; 341 [74.6%] with a clean-contaminated wound): 229 in the wound protector group and 228 in the surgical gauze group. The overall SSI rate in the intention-to-treat analysis was 15.7% (72 of 458 patients). The SSI rate for the wound protector was 10.9% (25 of 229 patients) compared with 20.5% (47 of 229 patients) with surgical gauze. The wound protector significantly reduced the risk of SSI, with a relative risk reduction (RRR) of 46.81% (95% CI, 16.64%-66.06%). The wound protector significantly decreased the SSI rate for clean-contaminated wounds (RRR, 43.75%; 95% CI, 3.75%-67.13%), particularly for superficial SSIs (RRR, 42.50%; 95% CI, 7.16%-64.39%). Length of hospital stay was similar in both groups (mean [SD], 15.2 [10.5] vs 15.3 [10.2] days), as were the overall postoperative complication rates (20.1% vs 18.8%).Conclusions and RelevanceThis randomized clinical trial found a significant reduction in SSI rates when a plastic wound protector was used during open GI surgery compared with surgical gaze, supporting the World Health Organization recommendation for use of wound protector devices in abdominal surgery.Trial RegistrationClinicalTrials.gov Identifier: NCT03170843
Advancing Alzheimer's research: Radiomics visualization of the default mode network in cerebral perfusion imaging
Authors: Fang Danzhou , Zhou Zhiming , Xiong Yalan , Fan Yongzeng , Li Yixuan , Zhao Huayi , Huang Jiahui , Yuan Gengbiao , Rao Maohua 
Published: April 2024

AbstractObjectiveAlzheimer's disease, an irreversible neurological condition, demands timely diagnosis for effective clinical intervention. This study employs radiomics analysis to assess image features in default mode network cerebral perfusion imaging among individuals with cognitive impairment.MethodsA radiomics analysis of cerebral perfusion imaging was conducted on 117 patients with cognitive impairment. They were divided into training and validation sets in a 7:3 ratio. Least Absolute Shrinkage and Selection Operator (LASSO) and Random Forest were employed to select and model image features, followed by logistic regression analysis of LASSO and Random Forest results. Diagnostic performance was assessed by calculating the area under the curve (AUC).ResultsIn the training set, LASSO achieved AUC of 0.978, Random Forest had an AUC of 0.933. In the validation set, LASSO had AUC of 0.859, Random Forest had AUC of 0.986. By conducting Logistic Regression analysis in combination with LASSO and Random Forest, we identified a total of five radiomics features, with four related to morphology and one to textural features, originating from the medial prefrontal cortex and middle temporal gyrus. In the training set, Logistic Regression achieved AUC of 0.911, while in the validation set, it attained AUC of 0.925.ConclusionThe medial prefrontal cortex and middle temporal gyrus are the two brain regions within the default mode network that hold the highest significance for Alzheimer's disease diagnosis. Radiomics analysis contributes to the clinical assessment of Alzheimer's disease by delving into image data to extract deeper layers of information.
Angiopoietin‐2 associates with poor prognosis in Moyamoya angiopathy
Authors: Gorla Gemma , Potenza Antonella , Carrozzini Tatiana , Pollaci Giuliana , Acerbi Francesco , Vetrano Ignazio G. , Ferroli Paolo , Canavero Isabella , Rifino Nicola , Bersano Anna , Gatti Laura 
Published: April 2024

AbstractObjectiveMoyamoya angiopathy (MA) is a rare cerebrovascular disorder characterized by recurrent ischemic/hemorrhagic strokes due to progressive occlusion of the intracranial carotid arteries. The lack of reliable disease severity biomarkers led us to investigate molecular features of a Caucasian cohort of MA patients.MethodsThe participants consisted of 30 MA patients and 40 controls. We measured cerebrospinal fluid (CSF) levels of angiogenic/inflammatory factors (ELISA). We then applied quantitative real‐time PCR on cerebral artery specimens for expression analyses of angiogenic factors. By an immunoassay based on microfluidic technology, we examined the potential correlations between plasma protein expression and MA clinical progression. A RNA interference approach toward Ring Finger Protein 213 (RNF213) and a tube formation assay were applied in cellular model.ResultsWe detected a statistically significant (p < 0.000001) up‐regulation of Angiopoietin‐2 (Ang‐2) in CSF and stenotic middle cerebral arteries (RQ >2) of MA patients compared to controls. A high Ang‐2 plasma concentration (p = 0.018) was associated with unfavorable outcome in a subset of MA patients. ROC curve analyses indicated Ang‐2 as diagnostic CSF biomarker (>3741 pg/mL) and prognostic plasma biomarker (>1162 pg/mL), to distinguish stable‐from‐progressive MA. Consistently, MA cellular model showed a significant up‐regulation (RQ >2) of Ang‐2 in RNF213 silenced condition.InterpretationOur results pointed out Ang‐2 as a reliable biomarker mirroring arterial steno‐occlusion and vascular instability of MA in CSF and blood, providing a candidate factor for patient stratification. This pilot study may pave the way to the validation of a biomarker to identify progressive MA patients deserving a specific treatment path.
In Situ Formed Li3N Networks by Soft Carbon‐Si3N4 for Superior All‐Solid‐State Lithium‐Metal Batteries
Authors: Wang Zhixuan , Mu Zhenliang , Ma Tenghuan , Yan Wenlin , Wu Dengxu , Li Yongxing , Yang Ming , Peng Jian , Xia Yu , Shi Shaochen , Chen Liquan , Li Hong , Wu Fan 
Published: April 2024

AbstractThe rapid growth of lithium (Li) dendrites has long hindered the development of all‐solid‐state lithium‐metal batteries (ASSLMB). Here, a composite soft carbon (SC)‐nano Si3N4 (SiN) interlayer (SC‐SiN) is designed for in situ formation of Li3N network (with high ionic conductivity/diffusivity) after lithium embedding in nano‐Si3N4, promoting the rapid migration of Li+ and guiding the metal Li to be deposited uniformly in a 3D manner within the interlayer. It can solve the problem of rapid consumption of Li+ and local charge accumulation at the interface of solid electrolyte and Li metal anode, thus avoiding the growth of Li‐dendrites. The resulting LCO/LPSCl/SC‐SiN‐Li ASSLMB achieves ultra‐high current density (12.5 mA cm−2) and ultra‐long cycle life (22 000 cycles with no degradation), as well as ultra‐high area capacity area capacity (15 mAh cm−2) and energy density (402.5 Wh kg−1), all of which break existing ASSLMB records. In addition, it is capable of 600 cycles at an areal capacity of 2.7 mAh cm−2 and 2 C (85.7% capacity retention). A pouch cell is also assembled to delivers high energy density (>320 Wh kg−1). These confirm the application potential of this configuration and are one of the most critical breakthroughs toward the commercialization of the ASSLMB.
The Role of the Molecular Encapsulation Effect in Stabilizing Hydrogen‐Bond‐Rich Gel‐State Lithium Metal Batteries
Authors: Xu Hantao , Deng Wei , Shi Lei , Long Juncai , Zhang Yongcai , Xu Lin , Mai Liqiang 
Published: April 2024

AbstractGel‐state polymer electrolytes with superior mechanical properties, self‐healing abilities and high Li+ transference numbers can be obtained by in situ polymerization of monomers with hydrogen‐bonding moieties. However, it is overlooked that the active hydrogen atoms in hydrogen‐bond donors experience displacement reactions with lithium metal in lithium metal batteries (LMBs), leading to corrosion of the lithium metal. Herein, it is discovered that the addition of hydrogen‐bond acceptors to hydrogen‐bond‐rich gel‐state electrolytes modulates the chemical activity of the active hydrogen atoms via the formation of hydrogen‐bonded intermolecular interactions. The characterizations reveal that the added hydrogen‐bond acceptors encapsulate the active hydrogen atoms to suppress the interfacial chemical corrosions of lithium metals, thereby enhancing the chemical stability of the polymer structure and interphase. With the employment of this strategy, a 1.1 Ah LiNi0.8Co0.1Mn0.1O2/Li metal pouch cell achieves stable cycling with 96.3 % capacity retention at 100 cycles. This new approach indicates a feasible path for achieving in situ polymerization of highly stable gel‐state‐based LMBs.
Analyse des Nativen Zustands von Proteinen durch Hochdruck NMR‐Spektroskopie unter Berücksichtigung des Ensembles Unstrukturierter Konformationen
Authors: Berner Frederic , Kovermann Michael 
Published: April 2024

AbstractDie Analyse druckabhängiger Änderungen der chemischen Verschiebung von Proteinen erlaubt es, Aussagen über strukturelle Fluktuationen zu machen, die diese bereits bei Umgebungsdruck aufweisen. Das Problem, allgemeine Effekte, die mit einer Erhöhung des hydrostatischen Drucks einhergehen, von strukturell bedingten Effekten auf die chemische Verschiebung zu trennen, wurde bisher durch das Einbeziehen der Eigenschaften unstrukturierter Modellpeptide angegangen. In dieser Arbeit wurden chemische Verschiebungen in 2D 1H‐15N‐HSQC Spektren von chemisch als auch druckdenaturierten Zuständen des Kälteschockproteins B aus Bacillus Subtilis (BsCspB) zugeordnet und ihre Abhängigkeit von Änderungen des hydrostatischen Drucks ausgewertet. Die durch hydrostatischen Druck denaturierte Polypeptidkette wurde verwendet, um allgemeine Effekte von strukturbedingten Effekten auf 1H und 15N chemische Verschiebungen zu trennen. Daraus resultierende Interpretationen der druckabhängigen Änderungen der chemischen Verschiebungen werden im strukturellen Kontext diskutiert. Es wurde festgestellt, dass das Ensemble unstrukturierter Konformationen von BsCspB anders auf eine Erhöhung des Drucks reagiert, als es unstrukturierte Modellpeptide tun. Daraus kann geschlussfolgert werden, dass der Ansatz, mit dem man allgemeine Effekte, die durch eine Erhöhung des Drucks hervorgerufen werden, abschätzt, die strukturellen Rückschlüsse ändert, die anhand einer Analyse von chemischen Verschiebungen getroffen werden.
Crown Ether Electrolyte Induced Li2O2 Amorphization for Low Polarization and Long Lifespan Li‐O2 Batteries
Authors: Li Meng , Wu Jiaxin , You Zichang , Dai Zhongqin , Gu Yuanfan , Shi Lei , Wu Meifen , Wen Zhaoyin 
Published: April 2024

AbstractLithium‐oxygen batteries possess an extremely high theoretical energy density, rendering them a prime candidate for next‐generation secondary batteries. However, they still face multiple problems such as huge charge polarization and poor life, which lay a significant gap between laboratory research and commercial applications. In this work, we adopt 15‐crown‐5 ether (C15) as solvent to regulate the generation of discharge products in lithium‐oxygen batteries. The coronal structure endows C15 with strong affinity to Li+, firmly stabilizes the intermediate LiO2 and discharge product Li2O2. Thus, the crystalline Li2O2 is amorphized into easily decomposable amorphous products. The lithium‐oxygen batteries assembled with 0.5 M C15 electrolyte show an increased discharge capacity from 4.0 mAh cm−2 to 5.7 mAh cm−2 and a low charge overpotential of 0.88 V during the whole lifespan at 0.05 mA cm−2. The batteries with 1 M C15 electrolyte can cycle stably for 140 cycles. Furthermore, the amorphous characteristic of Li2O2 product is preserved when matched with redox mediators such as LiI, with the charge polarization further decreasing to 0.74 V over a cycle life of 190 cycles. This provides new possibilities for electrolyte design to promote Li2O2 amorphization and reduce charge overpotential in lithium‐oxygen batteries.
C(sp3)−H (N‐Phenyltetrazole)thiolation as an Enabling Tool for Molecular Diversification
Authors: Zachmann Ashley K. Z. , Drappeau Justine A. , Liu Shubin , Alexanian Erik J. 
Published: April 2024

AbstractMethods enabling the broad diversification of C(sp3)−H bonds from a common intermediate are especially valuable in chemical synthesis. Herein, we report a site‐selective (N‐phenyltetrazole)thiolation of aliphatic and (hetero)benzylic C(sp3)−H bonds using a commercially available disulfide to access N‐phenyltetrazole thioethers. The thioether products are readily elaborated in diverse fragment couplings for C−C, C−O, or C−N construction. The C−H functionalization proceeds via a radical‐chain pathway involving hydrogen atom transfer by the electron‐poor N‐phenyltetrazolethiyl radical. Hexafluoroisopropanol was found to be essential to reactions involving aliphatic C(sp3)−H thiolation, with computational analysis consistent with dual hydrogen bonding of the N‐phenyltetrazolethiyl radical imparting increased radical electrophilicity to facilitate the hydrogen atom transfer. Substrate is limiting reagent in all cases, and the reaction displays an exceptional functional group tolerance well suited to applications in late‐stage diversification.
An Integrative Chemical Recycling Approach for Catalytic Oxidation of Epoxy Resin and in situ Separation of Degraded Products
Authors: Liu Lulu , Liu Xuehui , Li Xiaohui , Xu Shimei , Wang Yu‐Zhong 
Published: April 2024

AbstractAlthough many approaches have been proposed to recycling waste epoxy resin (EP), the separation of mixed degraded products remains a challenge due to their similar structures. To address this, we present a catalytic oxidation strategy that enables mild degradation of EP and in situ separation of degraded products through supramolecular interactions. The oxidative degradation relies on FeIV=O radicals with strong oxidizing properties, which are generated from the electron transfer of FeCl2 with reaction reagents. As the FeIV=O radicals attacked the C−N bonds of EP, EP was broken into fragments rich in active functional groups. Meanwhile, the FeIV=O radicals were reduced to iron ions that can coordinate with the carboxyl groups on the fragments. As a result, the degraded products with different carboxyl content can be effortlessly separated into liquid and solid phase by coordinating with the catalyst. The success of this work lays the foundation for high‐value application of degraded products and provides new design ideas for recycling waste plastics with complex compositions.
Enantiopure [6]‐Azairidahelicene by Dynamic Kinetic Resolution of a Configurationally Labile [4]‐Helicene
Authors: Pazos Ariadna , Cruz Carlos M. , Cuerva Juan M. , Rivilla Ivan , Cossío Fernando P. , Freixa Zoraida 
Published: April 2024

AbstractA pair of enantiopure [6]‐azairidahelicenes incorporating chirality at the metal center and on the helicenic ligand were synthesized by dynamic kinetic resolution (dkr) of a configurationally labile [4]‐helicenic ligand (4‐(2‐pyridyl)‐benzo[g]phenanthrene, L1H) using bis‐cyclometalated chiral‐at‐metal only iridium(III) precursors as chiral inductors. The origin of the observed dkr is attributed to the different conformation and stability of diastereomeric reaction intermediates formed during the cyclometalation process. The isolated enantiomers exhibited circularly polarized phosphorescence (CPP), with |gphos| values of 1.8×10−3.
Record High Iodate Anion Capture by a Redox‐Active Cationic Polymer Network
Authors: Guo Qi , Li Jie , Zhao Yuting , Li Lingyi , He Linwei , Zhao Fuqiang , Zhai Fuwan , Zhang Mingxing , Chen Long , Chai Zhifang , Wang Shuao 
Published: April 2024

AbstractAs a critical radioactive anionic contaminant, traditional adsorbents primarily remove iodate (IO3−) through ion exchange or hard acid‐hard base interactions, but suffer from limited affinity and capacity. Herein, employing the synergistic effect of ion exchange and redox, we successfully synthesized a redox‐active cationic polymer network (SCU‐CPN‐6, [C9H10O2N5 ⋅ Cl]n) by merging guanidino groups with ion‐exchange capability and phenolic groups with redox ability via a Schiff base reaction. SCU‐CPN‐6 exhibits a groundbreaking adsorption capacity of 896 mg/g for IO3−. The inferior adsorption capacities of polymeric networks containing only redox (~0 mg/g) or ion exchange (232 mg/g) fragments underscore the synergistic “1+1>2” effect of the two mechanisms. Besides, SCU‐CPN‐6 shows excellent uptake selectivity for IO3− in the presence of high concentrations of SO42−, Cl−, and NO3−. Meanwhile, a high distribution coefficient indicates its exemplary deep‐removal performance for low IO3− concentration. The synergic strategy not only presents a breakthrough solution for the efficient removal of IO3− but also establishes a promising avenue for the design of advanced adsorbents for diverse applications.
Building Co16‐N3‐Based UiO‐MOF to Expand Design Parameters for MOF Photosensitization
Authors: Guo Guang‐Chen , Zhao Jiong‐Peng , Guo Song , Shi Wen‐Xiong , Liu Fu‐Chen , Lu Tong‐Bu , Zhang Zhi‐Ming 
Published: April 2024

AbstractThe construction of secondary building units (SBUs) in versatile metal–organic frameworks (MOFs) represents a promising method for developing multi‐functional materials, especially for improving their sensitizing ability. Herein, we developed a dual small molecules auxiliary strategy to construct a high‐nuclear transition‐metal‐based UiO‐architecture Co16‐MOF‐BDC with visible‐light‐absorbing capacity. Remarkably, the N3− molecule in hexadecameric cobalt azide SBU offers novel modification sites to precise bonding of strong visible‐light‐absorbing chromophores via click reaction. The resulting Bodipy@Co16‐MOF‐BDC exhibits extremely high performance for oxidative coupling benzylamine (~100 % yield) via both energy and electron transfer processes, which is much superior to that of Co16‐MOF‐BDC (31.5 %) and Carboxyl @Co16‐MOF‐BDC (37.5 %). Systematic investigations reveal that the advantages of Bodipy@Co16‐MOF‐BDC in dual light‐absorbing channels, robust bonding between Bodipy/Co16 clusters and efficient electron‐hole separation can greatly boost photosynthesis. This work provides an ideal molecular platform for synergy between photosensitizing MOFs and chromophores by constructing high‐nuclear transition‐metal‐based SBUs with surface‐modifiable small molecules.