bluuebunny/crossref_metadata_2025_split huggingface dataset:
Insights From Omics in Lyme Disease
Authors: Bockenstedt Linda K , Belperron Alexia A 
Published: May 2024

Abstract
Lyme disease is a zoonotic infection due to Ixodes tick–transmitted Borrelia burgdorferi sensu lato spirochetes and the most common vector-borne disease in the Northern Hemisphere. Despite nearly 50 years of investigation, the pathogenesis of this infection and its 2 main adverse outcomes—postinfectious Lyme arthritis and posttreatment Lyme disease syndrome—are incompletely understood. Advancement in sequencing and mass spectrometry have led to the rapid expansion of high-throughput omics technologies, including transcriptomics, metabolomics, and proteomics, which are now being applied to human diseases. This review summarizes findings of omics studies conducted on blood and tissue samples of people with acute Lyme disease and its postinfectious outcomes.
Why Incorporate the ECHR? The Domestic Incentives of Human Rights Commitment
Authors: Schaffer Johan Karlsson 
Published: May 2024

Abstract
Why do consolidated democracies incorporate international human rights law (IHRL) treaties into national law? Existing research suggests contrastive accounts of the participation of democracies in IHRL regimes. While overall more likely to ratify, consolidated democracies are sometimes reluctant to accept demanding human rights commitments and less likely than both newly democratic and authoritarian regimes to incorporate international law in their constitutions. To theorize why established democracies commit to IHRL, this paper provides a comparative process tracing of the decisions to incorporate the European Convention on Human Rights (ECHR) into national law in Denmark and Sweden in the early 1990s. Why did these solid democracies with an exceptional commitment to human rights wait over 40 years to give domestic effect to a treaty they had helped create? Assessing rival theories of state commitment to human rights norms, the findings suggest that contextual developments in European law provided an opportunity for domestic political elites to seek insurance by incorporating the ECHR to place constraints on executive power. The argument qualifies claims about material strategizing or socialization to European norms as the primary drivers of incorporation.
Differentiation of Polygonatum Cyrtonema Hua from Different Geographical Origins by Near-Infrared Spectroscopy with Chemometrics
Authors: Hao Jing-Wen , Fan Xuan-Xuan , Li Yi-Na , Chen Nai-Dong , Ma Yun-Feng 
Published: May 2024

Abstract

Background
The identification of the geographical origin of Polygonatum cyrtonema Hua is of particular importance because the quality and market value of Polygonatum cyrtonema Hua from different production areas are highly variable due to differences in the growing environment and climatic conditions.

Objective
This study utilized near-infrared spectra (NIR) of Polygonatum cyrtonema Hua (n = 400) to develop qualitative models for effective differentiation of Polygonatum cyrtonema Hua from various regions.

Methods
The models were produced under different conditions to distinguish the origins distinctly. Ten preprocessing methods have been used to preprocess the original spectra (OS) and to select the most optimal spectral preprocessing method. Principal component analysis (PCA), partial least-squares discriminant analysis (PLS-DA), and orthogonal partial least squares discriminant analysis (OPLS-DA) were used to determine appropriate models. For simplicity, the pretreated full spectrum was calculated by different wavelength selection methods, and the four most significant variables were selected as discriminant indicator variables.

Results
The results show that Polygonatum cyrtonema Hua from different regions can be effectively distinguished using spectra from a series of samples analyzed by OPLS-DA. The accuracy of the OPLS-DA model is also satisfactory, with a good differentiation rate.

Conclusion
The study findings indicate the feasibility of using spectroscopy in combination with multivariate analysis to identify the geographical origins of Polygonatum cyrtonema Hua.

Highlights
The utilization of NIR spectroscopy combined with chemometrics exhibits high efficacy in discerning the provenance of herbal medicines and foods, thereby facilitating QA measures.
Molecular mechanisms of mechanosensing and plasticity of tendons and ligaments
Authors: Matsushima Takahide , Hiroshi Asahara 
Published: May 2024

Abstract
Tendons and ligaments, crucial components of the musculoskeletal system, connect muscles to bones. In the realm of sports, tendons and ligaments are vulnerable tissues, with injuries such as Achilles tendon rupture and anterior cruciate ligament tears directly impacting an athlete’s career. Furthermore, repetitive trauma and tissue degeneration can lead to conditions like secondary osteoarthritis, ultimately affecting the overall quality of life. Recent research highlights the pivotal role of mechanical stress in maintaining homeostasis within tendons and ligaments. This review delves into the latest insights on the structure of tendons and ligaments and the plasticity of tendon tissue in response to mechanical loads.
The externalities of immigration policies on migration flows: the case of an asylum policy
Authors: Guichard Lucas , Machado Joël 
Published: May 2024

Abstract
We analyze the externalities arising from a bilateral asylum policy—the list of safe origin countries—relying on a tractable model. Using self-collected monthly data, we estimate that including one origin country on the safe list of a given destination decreases asylum applications from that origin to that destination by 29 per cent. We use a counterfactual policy simulation to quantify the spillover effects occurring across origin and destination countries. Individuals from targeted origin countries move to alternative destinations. Individuals from untargeted origins divert from alternative destinations. The magnitude of the externalities depends on the size of the affected flows.
3D high-resolution radon transform based on a strong sparseLp–1norm and its applications
Authors: Shi Wei , Wang Weihong , Shi Ying , Chen Siyuan , Li Zhiwei , Wang Ning 
Published: May 2024

AbstractMultiple reflections are among the most challenging noises to suppress in seismic data, as they differ from effective waves only in terms of apparent velocity. Also, radon transform, an essential technique for attenuating multiple reflections, has been widely incorporated into various commercial software packages. Thus, this study introduces a 3D radon transform method based on the ${L_{p - 1}}$ norm to enhance sparsity-constraining capability in the transform domain, leveraging high-resolution radon transform techniques. Specifically, an iteratively reweighted least-squares algorithm is employed to obtain the transformed data in the radon domain. Given that the ${L_{p - 1}}$ norm was first applied to the Radon transform in seismic data processing methods, this paper theoretically demonstrates its powerful sparsity-constraining capability. Indeed, the proposed strategy enhances energy concentration in the radon transform domain, better separating primaries from multiples, and ultimately suppressing the multiples. Both model tests and real data indicate that the 3D radon transform constrained by the ${L_{p - 1}}$ norm outperforms existing high-resolution radon transform methods with sparsity constraints regarding energy concentration and effectiveness in multiple reflection attenuation.
Optimized expression of Peptidyl-prolyl cis/transisomerase cyclophilinB with prokaryotic toxicity from Sporothrix globosa
Authors: Hu Ling , Deng Baicheng , Wu Rong , Zhan Miaorong , Hu Xuchu , Huang Huaiqiu 
Published: May 2024

Abstract

Cyclophilin B (CypB), a significant member of immunophilins family with peptidyl-prolyl cis-trans isomerase (PPIase) activity, is crucial for the growth and metabolism of prokaryotes and eukaryotes. Sporothrix globosa (S. globosa), a principal pathogen in the Sporothrix complex, causes sporotrichosis. Transcriptomic analysis identified the cypB gene as highly expressed in S. globosa. Our previous study demonstrated that the recombinant Escherichia coli strain containing SgcypB gene failed to produce sufficient product when it was induced to express the protein, implying the potential toxicity of recombinant protein to the bacterial host. Bioinformatics analysis revealed that SgCypB contains transmembrane peptides within the 52 amino acid residues at the N-terminus and 21 amino acids near the C-terminus, and 18 amino acid residues within the cytoplasm. AlphaFold2 predicted a SgCypB 3D structure in which there is an independent PPIase domain consisting of a spherical extracellular part. Hence, we chose to express the extracellular domain to yield high-level recombinant protein with PPIase activity. Finally, we successfully produced high-yield, truncated recombinant CypB protein from S. globosa (SgtrCypB) that retained characteristic PPIase activity without host bacterium toxicity. This study presents an alternative expression strategy for proteins toxic to prokaryotes, such as SgCypB.

One-Sentence Summary
The recombinant cyclophilin B protein of Sporothrix globosa was expressed successfully by retaining extracellular domain with peptidyl-prolyl cis-trans isomerase activity to avoid toxicity to the host bacterium.
Assessing responses to heat in a range-shifting, nocturnal, flying squirrel
Authors: Hensley Vanessa R , Tan Ek Han , Gagne Emily , Levesque Danielle L 
Published: May 2024

Abstract
Over the last few decades North American flying squirrels (Glaucomys spp.) have experienced dramatic northward range shifts. Previous studies have focused on the potential effects of warming winter temperatures, yet the hypothesis that rising summer temperature had a role in these range shifts remained unexplored. We therefore sought to determine the effect of high environmental temperatures on the thermoregulation and energetics of flying squirrels in an area of the Northeast of North America with a recent species turnover. Unable to find a logistically feasible population of the northern species (Glaucomys sabrinus), we focused on Southern Flying Squirrels (G. volans). Using flow-through respirometry, we measured the relationship between metabolic rate, evaporative water loss, and body temperature at high ambient temperatures. We also measured core body temperature in free-ranging flying squirrels using temperature-sensitive data loggers. We detected no significant increase in metabolic rate up to ambient temperatures as high as 40 °C. However, evaporative water loss increased at temperatures above 36.2 °C. Free-ranging body temperature of flying squirrels followed a circadian pattern with a ~2 °C difference between active and resting phase modal body temperatures. Rest-phase body temperatures were influenced by environmental temperatures with higher resting temperatures observed on days with higher daily maximum ambient temperatures but not to an extent that energy or water costs were significantly increased during rest. We found that, due to a relatively high level of thermal tolerance, high ambient temperatures are unlikely to cause an energetic strain on Southern Flying Squirrels. However, these findings do not preclude negative impacts of high ambient temperatures on the northern species, and these may still play a role in the changing distributions of Glaucomys in North America.
Unmet needs in people with high-grade glioma: defining criteria for stepped care intervention
Authors: Faris Mona M , Dhillon Haryana M , Campbell Rachel , Halkett Georgia K B , Miller Annie , Chan Raymond J , Haydon Helen M , Sansom-Daly Ursula M , Koh Eng-Siew , Ownsworth Tamara , Nowak Anna K , Kelly Brian , Leonard Robyn , Pike Kerryn E , Legge Dianne M , Pinkham Mark B , Agar Meera R ,   BRAINS Program Group, Dhillon Haryana , Shaw Joanne , Agar Meera , Blackler Thea , Chan Raymond , Halkett Georgia , Haydon Helen , Legge Dianne , Leonard Robyn , Kelly Brian , Koh Eng-Siew , Nowak Anna , Ownsworth Tamara , Pike Kerryn , Rankin Nicole , Sansom-Daly Ursula , Rhee Joel , Miller Annie , Milley Kristi , Faris Mona , Campbell Rachel , Jeon Megan , Lion Katarzyna , Banks Hannah , Carlick Thomas , Chen Jill , Fazon Tiffany , Lyne Melinda , Virtue-Griffiths Sian , Vitangcol Kathryn , Buster Jessica , McDougall Emma , Shaw Joanne 
Published: May 2024

Abstract

Background
We aimed to define levels of unmet supportive care needs in people with primary brain tumor and to reach expert consensus on feasibility of addressing patients’ needs in clinical practice.

Methods
We conducted secondary analysis of a prospective cohort study of people diagnosed with high-grade glioma (n = 116) who completed the Supportive Care Needs Survey-Short Form during adjuvant chemoradiation therapy. Participants were allocated to 1 of 3 categories: no need (“no need” for help on all items), low need (“low need” for help on at least 1 item, but no “moderate” or “high” need), or moderate/high need (at least 1 “moderate” or “high” need indicated). Clinical capacity to respond to the proportion of patients needing to be prioritized was assessed.

Results
Overall, 13% (n = 5) were categorized as no need, 23% (n = 27) low need, and 64% (n = 74) moderate/high need. At least 1 moderate/high need was reported in the physical and daily living domain (42%) and the psychological (34%) domain. In recognition of health system capacity, the moderate/high need category was modified to distinguish between moderate need (“moderate” need indicated for at least 1 item but “high” need was not selected for any item) and high need (at least 1 “high” need indicated). Results revealed 24% (n = 28) moderate need and 40% (n = 46) high need. Those categorized as high need indicated needing assistance navigating the health system and information.

Conclusions
Using four step allocations resulted in 40% of patients indicating high need. Categories may facilitate appropriate triaging and guide stepped models of healthcare delivery.
Ebb and flow of network participation: flexibility, stability, and forms of flux in a purpose-oriented network
Authors: Lemaire Robin H , McKeague Lauren K , Sedgwick Donna 
Published: May 2024

Abstract
The flexibility/stability tension is a key challenge for purpose-oriented networks, especially salient with network participation. Because of the voluntary nature of networks, it is common for network participation to fluctuate, with participants entering, leaving, and returning over time for a variety of reasons. This fluctuation may challenge the stability that is key to network effectiveness. Yet, despite the salience of this tension, we know little about managing the ebb and flow of network participation. Driven by phenomenon-based theorizing, we draw on longitudinal participatory action research to examine participant attendance and contribution in monthly workgroup meetings over a four-year period of an early child education network. Combining interviews (n = 5), meeting attendance tracking (n = 37), and meeting observations (n = 30), we identify six types of flux stemming from individual, organizational, and system forces. We find these forces of flux support both flexibility and stability. Highlighting the duality of flexibility and stability, we explain how flexibility at one level may result in stability at another and vice versa. Our findings contribute to a greater understanding of how stability and flexibility are both valuable for networks and thus, the need to embrace the ebb and flow of participation.
Pharmacological potential of 4-dimethylamino chalcone against acute and neuropathic pain in mice
Authors: Marchon Isabela Souza dos Santos , Melo Evelynn Dalila do Nascimento , Botinhão Mirella da Costa , Pires Greice Nascimento , Reis João Vitor Rocha , de Souza Rodrigo Octavio Mendonça Alves , Leal Ivana Correa Ramos , Bonavita André Gustavo Calvano , Mendonça Henrique Rocha , Muzitano Michelle Frazão , da Silva Leandro Louback , do Carmo Paula Lima , Raimundo Juliana Montani 
Published: May 2024

Abstract

Objectives
This work investigated the acute antinociceptive effect of a synthetic chalcone, 4-dimethylamino chalcone (DMAC), as well as its effects on vincristine-induced peripheral neuropathy (VIPN) in mice.

Methods
The inhibitory activity of myeloperoxidase was assessed by measuring HOCl formation. Formalin and hot plate tests were used to study the acute antinociceptive effect of DMAC. VIPN was induced through the administration of vincristine sulphate (0.1 mg/kg, i.p., 14 days). Then, DMSO, DMAC (10 or 30 mg/kg; i.p.), or pregabalin (10 mg/kg, i.p.) were administered for 14 consecutive days. Thermal hyperalgesia and mechanical allodynia were evaluated before and after VIPN induction and on days 1, 3, 7, and 14 of treatment. Neurodegeneration and neuroinflammation were assessed through immunohistochemistry for NF200, iNOS, and arginase-1 within the sciatic nerve.

Key findings
DMAC inhibited myeloperoxidase activity in vitro and presented an acute antinociceptive effect in both formalin and hot plate tests, with the involvement of muscarinic and opioid receptors. Treatment with 30 mg/kg of DMAC significantly attenuated thermal hyperalgesia and mechanical allodynia and prevented macrophage proinflammatory polarisation in VIPN mice.

Conclusions
Our results show that DMAC, acting through different mechanisms, effectively attenuates VIPN.
Identification of the mechanistic basis of nitrogen responsiveness in two contrasting Setaria italica accessions
Authors: Bandyopadhyay Tirthankar , Maurya Jyoti , Bentley Alison R , Griffiths Howard , Swarbreck Stéphanie M , Prasad Manoj 
Published: May 2024

Abstract
Nitrogen (N) is a macronutrient limiting crop productivity with varied requirements across species and genotypes. Understanding the mechanistic basis of N responsiveness by comparing contrasting genotypes could inform the development and selection of varieties with lower N demands, or inform agronomic practices to sustain yields with lower N inputs. Given the established role of millets in ensuring climate-resilient food and nutrition security, we investigated the physiological and genetic basis of nitrogen responsiveness in foxtail millet (Setaria italica L.). We had previously identified genotypic variants linked to N responsiveness, and here we dissect the mechanistic basis of the trait by examining the physiological and molecular behaviour of N responsive (NRp-SI58) and non-responsive (NNRp-SI114) accessions at high and low N. Under high N, NRp-SI58 allocates significantly more biomass to nodes, internodes and roots, more N to developing grains, and is more effective at remobilizing flag leaf N compared with NNRp-SI114. Post-anthesis flag leaf gene expression suggests that differences in N induce much higher transcript abundance in NNRp-SI114 than NRp-SI58, a large proportion of which is potentially regulated by APETALA2 (AP2) transcription factors. Overall, the study provides novel insights into the regulation and manipulation of N responsiveness in S. italica.
The mitochondrial orf117Sha gene desynchronizes pollen development and causes pollen abortion in Arabidopsis Sha cytoplasmic male sterility
Authors: Dehaene Noémie , Boussardon Clément , Andrey Philippe , Charif Delphine , Brandt Dennis , Gilouppe Taillefer Clémence , Nietzel Thomas , Ricou Anthony , Simon Matthieu , Tran Joseph , Vezon Daniel , Camilleri Christine , Arimura Shin-ichi , Schwarzländer Markus , Budar Françoise 
Published: May 2024

Abstract
Cytoplasmic male sterility (CMS) is of major agronomical relevance in hybrid breeding. In gametophytic CMS, abortion of pollen is determined by the grain genotype, while in sporophytic CMS, it is determined by the mother plant genotype. While several CMS mechanisms have been dissected at the molecular level, gametophytic CMS has not been straightforwardly accessible. We used the gametophytic Sha-CMS in Arabidopsis to characterize the cause and process of pollen abortion by implementing in vivo biosensing in single pollen and mitoTALEN mutagenesis. We obtained conclusive evidence that orf117Sha is the CMS-causing gene, despite distinct characteristics from other CMS genes. We measured the in vivo cytosolic ATP content in single pollen, followed pollen development, and analyzed pollen mitochondrial volume in two genotypes that differed only by the presence of the orf117Sha locus. Our results showed that the Sha-CMS is not triggered by ATP deficiency. Instead, we observed desynchronization of a pollen developmental program. Pollen death occurred independently in pollen grains at diverse stages and was preceded by mitochondrial swelling. We conclude that pollen death is grain-autonomous in Sha-CMS and propose that mitochondrial permeability transition, which was previously described as a hallmark of developmental and environmental-triggered cell death programs, precedes pollen death in Sha-CMS.
Prevalence and genetic diversity of azole-resistant Malassezia pachydermatis isolates from canine otitis and dermatitis: A 2-year study
Authors: Álvarez-Pérez Sergio , Quevedo-Caraballo Sergio , García Marta E , Blanco José L 
Published: May 2024

Abstract
Despite previous reports on the emergence of Malassezia pachydermatis strains with decreased susceptibility to azoles, there is limited information on the actual prevalence and genetic diversity of azole-resistant isolates of this yeast species. We assessed the prevalence of azole resistance in M. pachydermatis isolates from cases of dog otitis or skin disease attended in a veterinary teaching hospital during a 2-year period and analyzed the ERG11 (encoding a lanosterol 14-α demethylase, the primary target of azoles) and whole genome sequence diversity of a group of isolates that displayed reduced azole susceptibility. Susceptibility testing of 89 M. pachydermatis isolates from 54 clinical episodes (1-6 isolates/episode) revealed low minimum inhibitory concentrations (MICs) to most azoles and other antifungals, but 11 isolates from six different episodes (i.e., 12.4% of isolates and 11.1% of episodes) had decreased susceptibility to multiple azoles (fluconazole, itraconazole, ketoconazole, posaconazole, ravuconazole, and/or voriconazole). ERG11 sequencing of these 11 azole-resistant isolates identified eight DNA sequence profiles, most of which contained amino acid substitutions also found in some azole-susceptible isolates. Analysis of whole genome sequencing (WGS) results revealed that the azole-resistant isolates from the same episode of otitis, or even different episodes affecting the same animal, were more genetically related to each other than to isolates from other dogs. In conclusion, our results confirmed the remarkable ERG11 sequence variability in M. pachydermatis isolates of animal origin observed in previous studies and demonstrated the value of WGS for disentangling the epidemiology of this yeast species.