bluuebunny/crossref_metadata_2025_split huggingface dataset:
Optimizing protein production in the One-Pot Pure system: insights into reaction composition and expression efficiency
Authors: Zhang Yan , Deveikis Matas , Qiu Yanping , Björn Lovisa , Martinez Zachary A. , Chou Tsui-Fen , Freemont Paul S. , Murray Richard M. 
Published: June 2024

ABSTRACTThe One-Pot PURE (Protein synthesisUsingRecombinantElements) system simplifies the preparation of traditional PURE systems by co-culturing and purifying 36 essential proteins for gene expression in a single step, thereby improving accessibility and affordability for widespread laboratory adoption and customization. However, replicating this protocol to match the productivity of traditional PURE systems can take considerable time and effort due to uncharacterized variability in the system’s biochemical composition. In this work, we observed unstable PURE protein expression inE. colistrains M15/pREP4 and BL21(DE3) and addressed this using glucose-mediated catabolite repression to minimize burdensome background expression. We also identified differences in optimal protein induction timing between these two strains, leading to growth incompatibility in co-culture, and observed proteolysis of PURE proteins expressed in M15/pREP4. We showed that consolidating all expression vectors into a protease-deficient BL21(DE3) strain could minimize proteolysis. This single-strain system also led to more uniform cell growth at the time of protein induction, improving the stoichiometry of critical translation initiation factors in the PURE reaction for efficient protein production. In addition to optimizing One-Pot PURE protein composition, we found that variations in commercial energy solution formulations could compensate for suboptimal PURE protein stoichiometry. Moreover, our study revealed significant differences in the expression capacity of commercially availableE. colitRNAs, suggesting the potential of optimizing tRNA codons to improve protein translation. Taken together, this work highlights the complex biochemical interplay influencing protein expression capacity in the One-Pot PURE system and presents strategies to improve its robustness and productivity.
Targeting SEZ6L2 in Colon Cancer: Efficacy of Bexarotene and Implications for Survival
Authors: Zheng Huajun , Zheng Jianying , Shen Yan 
Published: June 2024

AbstractBackgroundBexarotene, also recognized as Targretin, is categorized as a retinoid, a type of cancer drug. Nevertheless, the precise mechanisms of Bexarotene in relation to colon cancer remain unclear. In colon cancer, SEZ6L2 was suggested as one of the biomarkers and targets. This study presents a comprehensive exploration of the role of SEZ6L2 in colon cancer.MethodsWe utilized both TCGA data and a cohort of Chinese patients. In a meticulous analysis of 478 colon cancer cases, SEZ6L2 expression levels were examined in relation to clinical characteristics, staging parameters, and treatment outcomes. Additionally, we investigated the pharmacological impact of Bexarotene on SEZ6L2, demonstrating a significant downregulation of SEZ6L2 at both mRNA and protein levels in colon cancer patients following Bexarotene treatment.ResultsSEZ6L2 consistently overexpresses in colon cancer, serving as a potential universal biomarker with prognostic significance, validated in a diverse Chinese cohort. In vitro, SEZ6L2 promotes cell viability without affecting migration. Bexarotene treatment inhibits SEZ6L2 expression, correlating with reduced viability both in vitro and in vivo. SEZ6L2 overexpression accelerates declining survival rates in an in vivo context. Bexarotene’s efficacy is context-dependent, effective in parental cells but not with SEZ6L2 overexpression. Computational predictions suggest a direct SEZ6L2-Bexarotene interaction, warranting further experimental exploration.ConclusionThe study provides valuable insights into SEZ6L2 as a prognostic biomarker in colon cancer, revealing its intricate relationship with clinical parameters, treatment outcomes, and Bexarotene effects. Context-dependent therapeutic responses emphasize the nuanced understanding required for SEZ6L2’s role in colon cancer, paving the way for targeted therapeutic strategies.
Chemically induced cell plasticity enables the generation of high-fidelity embryo model
Authors: Li Huanhuan , Huang Jiahui , Guan Wei , Wu Jinyi , Luo Haiping , Chang Litao , Zhao Haiyong , Chen Chuanxin , Gao Yake , Zhang Jian , Silva José C. R. 
Published: June 2024

SummaryEmbryo models open exciting opportunities for improving our understanding of development and advancing medicine. However, current models depend on intricate procedures and the integration of separately generated cell types. Here, we aimed to establish a chemical-only strategy to induce cellular plasticity and generate embryo founder-like cells. Within 60 hours, chemical-induced pluripotent stem cells activated early lineage specifiers,Cdx2andGata6, while maintainingOct4/ Pou5f1expression. Following a second 36-hour chemical treatment, founder-like cells developed into all blastocyst fates and self-assembled into embryo-like structures. These progressed into high-fidelity late-streak gastrulation stages within 4.5 days, displaying primitive streak formation via epithelial-to-mesenchymal transition, along with germ-layer tissues, amnion, and ectoplacental cone. Fate trajectories and molecular signatures from founder-like cells to chemically-induced embryo model (ci-MO) were defined, revealing remarkable similarity up to Embryonic day (E) 7.5 embryo. Summarily, ci-MO offers a straightforward, rapid, small-molecule-only, and high-fidelity approach to study embryonic development and cell fate specification.HighlightsGeneration of a direct, efficient, and sophisticated chemically induced embryo model (ci-MO)Two-pulse chemical induction reprograms ESCs into founder-like cells capable of developing all embryo fatesGastrulating ci-MOs display high morphological and molecular accuracyci-MOs reconstruct embryo developmental trajectories
Molecular details and phospho-regulation of the CENP-T-Mis12 complex interaction during mitosis in DT40 cells
Authors: Takenoshita Yusuke , Hara Masatoshi , Nakagawa Reiko , Ariyoshi Mariko , Fukagawa Tatsuo 
Published: June 2024

AbstractTo establish bi-polar attachments of microtubules to sister chromatids, an inner kinetochore subcomplex, the constitutive centromere-associated network (CCAN), is assembled on centromeric chromatin and recruits the microtubule-binding subcomplex called the KMN network. Although it is important to characterize the interaction between CCAN and KMN, it is difficult to evaluate the significance of each interaction in cells, since CCAN proteins CENP-C and CENP-T independently bind to the Mis12 complex (Mis12C) of KMN. Here, we demonstrate molecular details of the CENP-T-Mis12C interaction using chicken DT40 cells lacking CENP-C-Mis12C interaction. Based on AlphaFold2 predictions, we identified two binding surfaces for the CENP-T-Mis12C interaction, demonstrating that each surface is critical for Mis12C recruitment and cell viability. This interaction via two interaction surfaces is cooperatively regulated by dual phosphorylation of Dsn1 (a Mis12C component) and CENP-T, ensuring robust CENP-T-Mis12C interaction and proper mitotic progression. These findings deepen our understanding of kinetochores assembly in cells.
Developing a model of digital transformation of university libraries based on meta-synthesis
Authors: Khoeini Soheila , Noruzi Alireza , Naghshineh Nader , Sheikhshoaei Fatemeh 
Published: June 2024

Purpose
The purpose of this study was to develop a model for the digital transformation of university libraries based on meta-synthesis.

Design/methodology/approach
The approach of this research was qualitative and used the Sandelowski and Barroso’s (2007) seven-step meta-synthesis method to systematically review the literature. The statistical population of the research includes all the scientific publications related to the foundations and dimensions of the digital transformation of university libraries, counting the scientific publications retrieved from six scientific databases in the period from 2004 to 2022, and was based on the critical appraisal skills programme and a screening process.

Findings
Based on the retrieved publications, 32 documents were selected for further review and analysis. First, a separate code was considered for all the factors extracted from the selected documents, and then, considering the concept of each of the codes, they were categorized into a similar concept. In this way, the research concepts were determined. Based on the analysis done with the help of the content analysis method, there are a total of seven main categories and 24 concepts, including digital culture (including: digital attitude, user-oriented, agility, participation and cooperation, creativity and innovation and learning digital skills of employees), digital librarian (containing: individual competence, knowledge competence, communication competence and skill competence), digital manager (comprising: individual competence, knowledge competence, skill competence), digital services and resources (including, optimally reorganizing library resources, providing digital services to users and providing digital services to the mother organization), digital technologies (containing: digital infrastructure, technological readiness and using digital technologies), support and cooperation of upstream institutions and the mother organization (dealing with: providing human resources, providing technological resources and equipment and making macro policies) and work process and development of digital strategies (comprising: digitalization of processes and development of library digital strategies).

Originality/value
Digital transformation is a complex and multi-faceted process, yet it is an indispensable endeavour for university libraries, and managers and librarians cannot be expected to be familiar with these concepts without training or study and then be able to move in the right direction towards the digital transformation of libraries. To the best of the authors’ knowledge, this is the first study to develop a model for the digital transformation of university libraries. The results of this research indicate the effective factors and requirements needed for the digital transformation of university libraries and address the importance of understanding the concepts for managers and librarians.
How does financial inclusion act as a catalyst for reducing financial crime among women?
Authors: Akram Tooba , Muhammad Naveed , RamaKrishnan Suresh 
Published: June 2024

Purpose
This study aims to review financial inclusion as a catalyst to reduce financial scams and frauds faced by women in the five largest US states by population and proposed measures encouraging women’s financial safety.

Design/methodology/approach
Recognizing the unique socioeconomic landscape, the study seeks responses through a survey questionnaire from 4,113 women respondents analyzed by using a basic mixed-methods approach, including quantitative surveys analyzed through SPSS and qualitative short interviews thematically analyzed by using Nvivo.

Findings
The review results show that 94% of women believe that financial inclusion can protect them from scams and fraud. Also, it has been observed that financial crimes disproportionately affect women, often stemming from a desire to conceal such activities from close family members and partners. Older women, housewives and those from financially depressed areas need more financial inclusion plans to curb financial fraud.

Social implications
The proposed measures may have positive social and economic implications on the females residing in the financially depressed areas.

Originality/value
The study represents the authors’ original contribution, examining the role of financial inclusion in preventing women from engaging in financial crimes.
e–WOM and app stores in Iran: how online reviews influence purchase intention of paid mobile apps
Authors: Asadi Damavandi Azade , Ha Louisa 
Published: June 2024

Purpose
This study aims to examine the relative importance of the impact of central and peripheral route processing of online reviews (ORs) on the acceptance of paid mobile apps among consumers in a fully Islamic market. The authors used the elaboration likelihood model (ELM) and technology acceptance model (TAM) to explain how ORs influence users’ perception and purchase intention of paid mobile apps.

Design/methodology/approach
An online survey of 384 Iranian paid mobile app users was conducted to compare the role of overall product ranking, argument quality and information quantity on the perceived usefulness (PU) and perceived ease of use (PEU) of mobile apps and purchase intentions of paid mobile apps.

Findings
Results show that only overall product ranking and argument quality influence perceived PEU and PU of paid mobile apps. The quantity of reviews on the app store does not affect perception of the app. The path model supports the mediating role of PU and PEU on information processing and attitude toward the app and purchase intention.

Practical implications
To Islamic markets such as Iran, this study shows that paid mobile app marketers need to monitor closely the reviews in the app stores and make prompt response to negative reviews even if there are only a few reviews because users did not pay attention to the quantity of reviews. The overall ranking and argument quality strongly affect perception and purchase intention of paid apps.

Originality/value
To the best of the authors’ knowledge, this is the first study that combines ELM and TAM in determining the relative importance of central and peripheral processing of information in online product reviews, specifically for paid mobile apps in app stores in a large Islamic market.
Switching intention of conventional bank customers to Sharia bank based on push-pull-mooring theory
Authors: Monoarfa Hilda , Al Adawiyah Rumaisah Azizah , Prananta Widya , Sadat Andi Mohammad , Vakhroh Disya Allifah 
Published: June 2024

Purpose
This study aims to see the variables of customer satisfaction level, attractiveness of alternatives, subjective norms and level of religiosity of conventional bank customers in West Java and their effect on switching intentions.

Design/methodology/approach
A causality description technique with a quantitative approach is used in this research. The analytical technique used is partial least square-structural equation modeling with a sample of 320 respondents from conventional bank customers in West Java.

Findings
The results revealed that the level of customer satisfaction and subjective norms were in the medium category, whereas the level of alternative attractiveness, religiosity and switching intentions was in high level. The level of customer satisfaction harms switching intentions, whereas the level of alternative attractiveness and religiosity variables have a positive effect on switching intentions. In contrast, the subjective norm variable does not affect switching intentions. Therefore, customer satisfaction, the attractiveness of alternatives and religiosity are essential points to cause someone to have the intention to switch.

Practical implications
For stakeholders, especially the government or the Islamic banking industry, to improve the climate of the Islamic finance industry in Indonesia, in particular, to increase knowledge and insight from the public regarding the intention to switch conventional bank customers to Islamic banks.

Originality/value
The use of the religiosity variable as an independent variable on switching intentions is still rarely done. Hence, the authors combine this variable with customer satisfaction, alternative attractiveness and subjective norms. The update in this study also places conventional bank customers included in the millennial generation and generation Z categories who live in the province of West Java as research subjects.
Paid Leave Mandates and Care for Older Parents
Authors: ARORA KANIKA , WOLF DOUGLAS A. 
Published: June 2024

Policy Points
We examined the effect of the Paid Family Leave policy (PFL) and Paid Sick Leave policy (PSL) on care provision to older parents.
We found that PSL adoption led to an increase in care provision, an effect mainly attributable to respondents in states/periods when PSL and PFL were concurrently offered. Some of the strongest effects were found among women and unpartnered adult children.
PFL adoption by itself was not associated with care provision to parents except when PFL also offered job protection.
Paid leave policies have heterogeneous effects on eldercare and their design and implementation should be carefully considered.
ContextFamily caregivers play a critical role in the American long‐term care system. However, care responsibilities are known to potentially conflict with paid work, as about half of family caregivers are employed. The federal Family and Medical Leave Act passed by the US Congress in 1993 provides a nonuniversal, unpaid work benefit. In response, several states and localities have adopted the Paid Family Leave policy (PFL) and Paid Sick Leave policy (PSL) over the last two decades. Our objective is to examine the effect of these policies on the probability of personal care provision to older parents.MethodsThis study used longitudinal data from the Health and Retirement Study (1998‐2020). Difference‐in‐differences regression models were estimated to examine associations between state‐ and local‐level PFL and PSL mandates and personal care provision to older parents. We analyzed heterogeneous effects by the type of paid leave exposure (provision of job protection with PFL and availability of both PSL and PFL [with or without job protection] concurrently). We also examined results for different population subgroups.FindingsPSL implementation was associated with a four‐ to five‐percentage point increase in the probability of personal care provision. These effects were mainly attributable to respondents in states/periods when PSL and PFL were concurrently offered. The strongest effects were found among adult children who were employed at baseline, women, younger, unpartnered, and college educated. PFL implementation by itself was not associated with care provision to parents except when the policy also offered job protection.ConclusionsPaid leave policies have heterogeneous impacts on personal care provision, potentially owing to differences in program features, variation in caregiving needs, and respondent characteristics. Overall, the results indicate that offering paid sick leave and paid family leave, when combined with job protection, could support potential family caregivers.
The financial development and renewable energy consumption nexus in Africa: Does the quality of governance matter?
Authors: Alinsato Alastaire S. , Dossou Toyo Amègnonna Marcel , Dossou Pascal K. , N. Kambaye Emmanuelle , Asongu Simplice A. 
Published: June 2024

AbstractThe impact of financial development (FD) on renewable energy consumption has been extensively examined in recent years. Nevertheless, studies on the moderation of quality of governance in the financial development and renewable energy consumption nexus are sparse. By filling the gap in the literature relating to energy economicsc, this study investigates the moderating effect of the quality of governance on the relationship between financial development and consumption of renewable energy for a panel of 33 African countries over the period between 2000 and 2020. The fully modified ordinary least squares (FMOLS) estimation technique is used to account for cointegration and cross‐sectional dependence. The results unveil that the impact of governance quality and financial development on renewable energy consumption is negative and statistically significant. Moreover, the results reveal that the FD‐governance quality interactions are significant and negative. Governance quality thresholds at which the negative incidence of financial development on renewable energy consumption is completely nullified are provided.
Current therapeutic approach of chronic kidney disease‐mineral and bone disorder
Authors: Zaimi Maria , Grapsa Eirini 
Published: June 2024

AbstractChronic kidney disease (CKD) has emerged as one of the leading noncommunicable diseases affecting >10% of the population worldwide. Bone and mineral disorders are a common complication among patients with CKD resulting in a poor life quality, high fracture risk, increased morbidity and cardiovascular mortality. According to Kidney Disease: Improving Global Outcomes, renal osteodystrophy refers to changes in bone morphology found in bone biopsy, whereas CKD‐mineral and bone disorder (CKD‐MBD) defines a complex of disturbances including biochemical and hormonal alterations, disorders of bone and mineral metabolism and extraskeletal calcification. As a result, the management of CKD‐MBD should focus on the aforementioned parameters, including the treatment of hyperphosphatemia, hypocalcemia, abnormal PTH and vitamin D levels. Regarding the bone fragility fractures, osteoporosis and renal osteodystrophy, which constitute the bone component of CKD‐MBD, anti‐osteoporotic agents constitute the mainstay of treatment. However, a thorough elucidation of the CKD‐MBD pathogenesis is crucial for the ideal personalized treatment approach. In this paper, we review the pathology and management of CKD‐MBD based on the current literature with special attention to recent advances.
Optimizing placement of bioretention systems in the US Puget Sound region
Authors: Mahat Anish , Wu Joan Q. , Jayakaran Anand D. , Dahal M. Samrat , Ewing Robert P. 
Published: June 2024

AbstractThe Puget Sound Basin, US Pacific Northwest, is experiencing rapid population and urban growth. This growth adversely impacts local ecosystems, especially the spawning and rearing habitat for several salmonid species. Sustainable urban design strategies such as green stormwater infrastructure (GSI) are required in the region to manage stormwater onsite when new development occurs. However, the effectiveness of any GSI depends on its location relative to where stormwater is produced. This study aimed to develop a Geographic Information System (GIS)‐based framework for the optimal placement of GSI, specifically bioretention systems. We computed the Hydrologic Sensitivity Index (λHSI, indicating runoff generation potential at a landscape location) for the lower Puyallup River Watershed study area. The index and federal and state feasibility criteria were used to identify suitable sites for bioretention systems. The suitability of identified sites was verified through ground‐truthing, including soil sampling and infiltration testing. We found that 2.5% of the watershed area was suitable for bioretention, concentrated in the center and north of the study watershed. The method described in this study can be readily applied to watersheds for which spatial data (topography, soil, and land use) are available. We recommend choosing locations with high λHSI when resources are limited since these locations contribute most to runoff generation and urban flooding.