bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prevalence rate for inherited thrombophilia in patients with chronic and recurrent venous leg ulceration
Authors: Wiszniewski Adam , Bykowska Ksenia , Bilski Radoslaw , Jaśkowiak Wojciech , Proniewski Jacek 
Published: August 2011

AbstractThe aim of the study was to determine the prevalence rate for inherited thrombophilia (IT) in patients with chronic (CVU) and recurrent venous leg ulceration. We also investigated and evaluated the severity of the clinical pattern of CVU in patients with and without IT. We examined 110 patients with CVU (the study group) and 110 healthy subjects (the control group). We prepared a questionnaire to be completed by each study participant. Ultrasound Doppler color imaging or/and duplex ultrasonography was performed to evaluate the efficiency of the venous system. The ankle‐brachial index was calculated to determine the efficiency of the arterial system. We examined both groups for the presence of IT. IT was diagnosed in 30% of study group and in 1.8% of control group. Our diagnoses of deep vein thrombosis (DVT) were based on medical interviews, physical examinations, and an ultrasonography of the venous system and concerned 64 study group patients (58.2%), 35 of whom (31.8%) experienced recurrent DVT. Proximal and/or distal DVT was determined in an interview and/or by an ultrasonography performed for all patients with CVU and IT. In 94% of these patients, DVT was recurrent, and in 88% of patients with CVU and IT, we observed recurrent DVT and CVU. It recurred more often and persisted longer when compared to patients with CVU and no IT, despite similar management. No differences were observed in ulcer size, localization, or pain level related to ulceration between patients with CVU and IT and those with CVU and no IT.
Landscape matrix modifies richness of plants and insects in grassland fragments
Authors: Öckinger Erik , Lindborg Regina , Sjödin N. Erik , Bommarco Riccardo 
Published: August 2011

There is an increasing awareness that not only area and isolation, but also the characteristics of the landscape surrounding habitat patches influence population persistence and species diversity in fragmented landscapes. In this study, we examine the effects of grassland fragmentation and land use in the landscape matrix (on a 2 km scale) on species richness of plants, butterflies, bees and hoverflies. These organisms were studied in replicated remnant patches of different sizes and isolation, embedded in landscapes dominated either by forest, arable land or a mix of these.We found positive effects of patch area on species richness of the three insect taxa, but not of plants. Isolation had a negative effect only on hoverflies. Matrix type had contrasting effects on the studied taxa. Species richness of plants and butterflies was lowest in patches in landscapes dominated by arable land and highest in forest‐dominated landscapes. For hoverflies, the negative effect of small patch area was strongest in forest‐dominated landscapes, and there was a similar non‐significant trend for bees. Our study shows the importance of considering matrix characteristics when studying responses to habitat fragmentation. Differences in matrix response among organism groups probably impinge on differing mechanisms. A forest matrix is likely to provide additional resources for butterflies but either constitute a barrier to dispersal or deprive resources as compared to an arable matrix for hoverflies. Enhanced plant diversity in grassland patches embedded in forested landscapes can be explained by habitat generalists more easily invading these patches, or by an unpaid extinction debt in these landscapes.
Clinical analysis of Chinese limb osteosarcoma patients treated by two combinations of methotrexate, cisplatin, doxorubicin and ifosfamide
Authors: LIN Feng , WANG Qiong , YU Wenxi , TANG Lina , ZHENG Shuier , SUN Yuanjue , SHEN Zan , YAO Yang , DONG Yang 
Published: August 2011

AbstractAims:  The objective of this study was to investigate the efficacy and toxic side effects of two combinations of methotrexate, cisplatin, doxorubicin and ifosfamide on treating Chinese osteosarcoma patients.Methods:  A retrospective analysis was conducted of 185 osteosarcoma patients treated with the four drugs mentioned above. A total of 93 patients received the Italian therapeutic treatment (IOR‐OS/N‐5: Instituto Ortopedic Rizzoli‐Section of Osteosacorma/Neoadjuvant chemotherapy‐5) and the remaining 92 patients received the therapeutic treatment optimized by our department. We compared the efficacy and toxic side effects of these two therapies.Results:  The limb salvage rates, 3‐year recurrence rates and 3‐year metastasis rates of IOR‐OS/N‐5 and our optimized treatment were 52.7 and 58.7%; 20.9 and 11.4% and 44.1 and 27.2%, respectively. The 3‐year survival rates and the 3‐year disease‐free survival rates of IOR‐OS/N‐5 and our optimized treatments were 62.4 and 78.4%; and 53.6 and 67.8%, respectively. Overall survival and disease‐free survival rates were significantly different between these two treatments (P = 0.032 and 0.024, respectively). Liver function with degree III and IV damage accounted for 35.2 and 16.6% of the total adverse reactions in the IOR‐OS/N‐5 and our optimized treatment groups, respectively. Degree III–IV neutropenia accounted for 22.6 and 40.0%, respectively, in the IOR‐OS/N‐5 and optimized treatments.Conclusion:  The optimization of MTX, DDP, DOX and IFO based on Chinese patients' physiology increased the tolerance and efficacy for the treatment of osteosarcoma.
Surgical reconstruction of a severely comminuted mandibular fracture in a horse
Authors: Caldwell F. J. , Davis H. A. 
Published: August 2011

SummaryA Palomino gelding presented for evaluation of a mandibular fracture and physical examination revealed swelling and excoriation of the skin over the ventral mandible. Upon further examination, malalignment of the incisors was appreciated in addition to instability and crepitance of both the rostral aspect and vertical ramus of the mandible. Radiographic examination revealed a comminuted fracture within the interdental space and fractures of both vertical ramii of the mandible. Under general anaesthesia via tracheotomy intubation, the fractures of the vertical ramii were reduced and compression plating techniques used along the caudal‐lateral border of both ramii for stabilisation. Intraoral tension band wires were used for repair of the interdental space fracture. A type II external skeletal fixator using Steinmann pins and polymethyl methacrylate connecting bars was applied to increase stability. The horse was maintained on broad spectrum antibiotics and phenylbutazone and discharged following 6 days of hospitalisation. Radiographs were taken 10 weeks after surgery and revealed evidence of adequate healing. On follow‐up 20 months later, the horse was reported to have no difficulties eating and was maintaining its bodyweight. This case is unique due to the severity of the mandibular fracture. The authors are unaware of any previous reports of such a severely comminuted mandibular fracture and its successful reconstruction employing multiple surgical techniques.
Contribution of local fishermen to improving knowledge of the marine ecosystem and resources in the Republic of Guinea, West Africa
Authors: Le Fur Jean , Guilavogui Athanase , Teitelbaum Antoine 
Published: August 2011

We assessed the quality of fishermen’s local ecological knowledge, or LEK, as a potential source of information about coastal ecosystem functioning in the Republic of Guinea. Interviews were conducted by means of partial immersion or repeated surveys at six landing sites. In each site and for each topic, discussions were conducted with 3 to 15 individual fishermen and 1 to 10 groups of fishermen. Knowledge was obtained about habitats, substrate preferences, the location of nurseries, reproductive cycles, fish diet, and the trophic network of the Sciaenid community, the major resource for fisheries in this area. We systematically compared the reliability of the information collected with that of scientific information collected in parallel surveys or published data. The contribution of LEK should be considered on a case-by-case basis. Indeed, LEK could be used as (i) a supplementary source of scientific studies (seabed description), (ii) a basis for new scientific investigation (species reproductive cycle), (iii) the only possibility to obtain information (nursery location), (iv) a surrogate to scientific surveys providing an identical level of validity (fish diets) or a satisfactory proxy (trophic network) in a context of limited resources and data in which wide-ranging knowledge relating to the entire coast must be obtained.
Down, up, down and “smolting” twice? Seasonal movement patterns by juvenile steelhead (Oncorhynchus mykiss) in a coastal watershed with a bar closing estuary
Authors: Hayes Sean A. , Bond Morgan H. , Hanson Chad V. , Jones Andrew W. , Ammann Arnold J. , Harding Jeffrey A. , Collins Alison L. , Perez Jeffrey , MacFarlane R. Bruce 
Published: August 2011

We investigated emigration behavior, habitat use, and Na+,K+-ATPase activity levels of juvenile steelhead trout (sea-run rainbow trout; Oncorhynchus mykiss ) in Scott Creek, a small coastal watershed with a seasonally closed estuary in central California, USA. Migratory fish moved downstream in the spring with elevated Na+,K+-ATPase activity levels. Larger fish (>150 mm) moved downstream during February and March, leaving the stream, whereas fish moving between April and June were typically smaller and tended to recruit to the estuary. The Na+,K+-ATPase activity levels of estuarine fish varied during the summer as a function of salinity and temperature, but overall levels declined from peak spring values. Many summer recruits were observed retreating upstream into the watershed when estuarine water quality declined in the fall. Rather than entering the ocean when winter storms reconnected the estuary with the ocean in early winter, many migrated downstream several months later during subsequent springs. The largest smolts observed (>190 mm fork length (LF)) were primarily those that had reared in the estuary the previous summer. Smolts were observed making a single migration from the upper watershed, but they were smaller (~120–190 mm LF) with potentially reduced marine survival. In summary, we observed fish moving between freshwater and estuarine habitats seasonally and adjusting their osmoregulatory physiology as needed.
Fine-scale population genetic structure of the yellow perchPerca flavescensin Lake Erie
Authors: Sepulveda-Villet Osvaldo J. , Stepien Carol A. 
Published: August 2011

Discerning the genetic basis underlying fine-scale population structure of exploited native species and its relationship to management units is a critical goal for effective conservation. This study provides the first high-resolution genetic test of fine-scale relationships among spawning groups of the yellow perch Perca flavescens . Lake Erie yellow perch stocks comprise valuable sport and commercial fisheries and have fluctuated extensively owing to highly variable annual recruitment patterns. Fifteen nuclear DNA microsatellite loci are analyzed for 569 individuals from 13 primary Lake Erie spawning sites and compared with those spawning in Lakes St. Clair and Ontario. Additional comparisons test for possible genetic differences between sexes and among size–age cohorts. Results demonstrate that yellow perch spawning groups in Lake Erie are genetically distinguishable and do not differ between sexes and among age cohorts. Population genetic structure does not follow a genetic isolation with geographic distance pattern, and some spawning groups contribute more to overall lake-wide genetic diversity than do others. Partitioning of the yellow perch’s genetic structure shows little congruence to lake basins or to current management units. Our findings underlie the importance of understanding spawning habitat and behavior to conserve the genetic stock structure of a key fishery.
Theoretical studies on ClOO — electronic spectra, ionization potential, and electron affinity
Authors: Grein Friedrich 
Published: August 2011

Vertical excitation energies and oscillator strengths of doublet and quartet states of ClOO, covering doublet states up to 7.5 eV and quartet states up to 9 eV, were obtained by multireference configuration interaction calculations. Strong absorptions from the X2A″ ground state are predicted at 186 and 235 nm. Experimentally, a maximum has been found near 248 nm. The grouping of excited states, with twelve low-lying doublet states and three low-lying quartet states, is explained by the interaction of the 2P ground state of Cl with the π*2 states 3Σg–, 1Δg, and 1Σg+ of O2. Potential energy curves for Cl–O separation at fixed O–O distance and ClOO angle show the lower states to be repulsive (with the exception of the ground state), and higher states to have minima due to avoided crossings. The lowest Rydberg states are expected around 8.5 eV. Adiabatic ionization potentials (IP) and electron affinities (EA) of ClOO were obtained by density functional and coupled cluster methods, with values of 11.60–11.79 eV for IP and 3.56–3.79 eV for EA.
Celecoxib Promotes c-FLIP Degradation through Akt-Independent Inhibition of GSK3
Authors: Chen Shuzhen , Cao Wei , Yue Ping , Hao Chunhai , Khuri Fadlo R. , Sun Shi-Yong 
Published: August 2011

Abstract
Celecoxib is a COX-2 inhibitor that reduces the risk of colon cancer. However, the basis for its cancer chemopreventive activity is not fully understood. In this study, we defined a mechanism of celecoxib action based on degradation of cellular FLICE-inhibitory protein (c-FLIP), a major regulator of the death receptor pathway of apoptosis. c-FLIP protein levels are regulated by ubiquitination and proteasome-mediated degradation. We found that celecoxib controlled c-FLIP ubiquitination through Akt-independent inhibition of glycogen synthase kinase-3 (GSK3), itself a candidate therapeutic target of interest in colon cancer. Celecoxib increased the levels of phosphorylated GSK3, including the α and β forms, even in cell lines, where phosphorylated Akt levels were not increased. Phosphoinositide 3-kinase inhibitors abrogated Akt phosphorylation as expected but had no effect on celecoxib-induced GSK3 phosphorylation. In contrast, protein kinase C (PKC) inhibitors abolished celecoxib-induced GSK3 phosphorylation, implying that celecoxib influenced GSK3 phosphorylation through a mechanism that relied upon PKC and not Akt. GSK3 blockade either by siRNA or kinase inhibitors was sufficient to attenuate c-FLIP levels. Combining celecoxib with GSK3 inhibition enhanced attenuation of c-FLIP and increased apoptosis. Proteasome inhibitor MG132 reversed the effects of GSK3 inhibition and increased c-FLIP ubiquitination, confirming that c-FLIP attenuation was mediated by proteasomal turnover as expected. Our findings reveal a novel mechanism through which the regulatory effects of c-FLIP on death receptor signaling are controlled by GSK3, which celecoxib acts at an upstream level to control independently of Akt. Cancer Res; 71(19); 6270–81. ©2011 AACR.
Loss of Transcription Factor KLF5 in the Context of p53 Ablation Drives Invasive Progression of Human Squamous Cell Cancer
Authors: Yang Yizeng , Nakagawa Hiroshi , Tetreault Marie-Pier , Billig Janelle , Victor Noel , Goyal Abha , Sepulveda Antonia R. , Katz Jonathan P. 
Published: August 2011

Abstract
Squamous cell cancers account for more than half of all human cancers, and esophageal cancer is the sixth leading cause of cancer death worldwide. The majority of esophageal squamous cell carcinomas have identifiable p53 mutations, yet the same p53 mutations are found at comparable frequencies in precancerous dysplasia, indicating that transformation requires additional somatic changes yet to be defined. Here, we show that the zinc finger transcription factor Krüppel-like factor 5 (KLF5) transactivates NOTCH1 in the context of p53 mutation or loss. KLF5 loss limited NOTCH1 activity and was sufficient on its own to transform primary human keratinocytes harboring mutant p53, leading to the formation of invasive tumors. Restoration of NOTCH1 blocked transformation of KLF5-deficient and p53-mutant keratinocytes. Although human dysplastic epithelia accumulated KLF5, KLF5 expression was lost concurrently with NOTCH1 in squamous cell cancers. Taken together, these results define KLF5 loss as a critical event in squamous cell transformation and invasion. Our findings suggest that KLF5 may be a useful diagnostic and therapeutic target in esophageal squamous carcinomas and possibly more generally in other cancers associated with p53 loss of function. Cancer Res; 71(20); 6475–84. ©2011 AACR.
Assembly and Initial Characterization of a Panel of 85 Genomically Validated Cell Lines from Diverse Head and Neck Tumor Sites
Authors: Zhao Mei , Sano Daisuke , Pickering Curtis R. , Jasser Samar A. , Henderson Ying C. , Clayman Gary L. , Sturgis Erich M. , Ow Thomas J. , Lotan Reuben , Carey Thomas E. , Sacks Peter G. , Grandis Jennifer R. , Sidransky David , Heldin Nils Erik , Myers Jeffrey N. 
Published: August 2011

AbstractPurpose: Human cell lines are useful for studying cancer biology and preclinically modeling cancer therapy, but can be misidentified and cross-contamination is unfortunately common. The purpose of this study was to develop a panel of validated head and neck cell lines representing the spectrum of tissue sites and histologies that could be used for studying the molecular, genetic, and phenotypic diversity of head and neck cancer.Methods: A panel of 122 clinically and phenotypically diverse head and neck cell lines from head and neck squamous cell carcinoma, thyroid cancer, cutaneous squamous cell carcinoma, adenoid cystic carcinoma, oral leukoplakia, immortalized primary keratinocytes, and normal epithelium was assembled from the collections of several individuals and institutions. Authenticity was verified by carrying out short tandem repeat analysis. Human papillomavirus (HPV) status and cell morphology were also determined.Results: Eighty-five of the 122 cell lines had unique genetic profiles. HPV-16 DNA was detected in 2 cell lines. These 85 cell lines included cell lines from the major head and neck primary tumor sites, and close examination shows a wide range of in vitro phenotypes.Conclusions: This panel of 85 genomically validated head and neck cell lines represents a valuable resource for the head and neck cancer research community that can help advance understanding of the disease by providing a standard reference for cell lines that can be used for biological as well as preclinical studies. Clin Cancer Res; 17(23); 7248–64. ©2011 AACR.
Polymeric Nanoparticle-Encapsulated Hedgehog Pathway Inhibitor HPI-1 (NanoHHI) Inhibits Systemic Metastases in an Orthotopic Model of Human Hepatocellular Carcinoma
Authors: Xu Yang , Chenna Venugopal , Hu Chaoxin , Sun Hai-Xiang , Khan Mehtab , Bai Haibo , Yang Xin-Rong , Zhu Qing-Feng , Sun Yun-Fan , Maitra Anirban , Fan Jia , Anders Robert A. 
Published: August 2011

Abstract
Purpose: To illustrate the prognostic significance of hedgehog (Hh) signaling in patients with hepatocellular carcinoma (HCC) and to evaluate the efficacy of a novel nanoparticle-encapsulated inhibitor of the Hh transcription factor, Gli1 (NanoHHI) using in vitro and in vivo models of human HCCs.
Experimental Design: Patched1 (Ptch1) expression was detected in tumor tissue microarrays of 396 patients with HCC who underwent curative surgical resection during February 2000 to December 2002. Prognostic significance was assessed using Kaplan–Meier survival estimates and log-rank tests. The effects of NanoHHI alone and in combination with sorafenib were investigated on HCC cell lines. Primary HCC tumor growth and metastasis were examined in vivo using subcutaneous and orthotopic HCC xenografts in nude mice.
Results: Elevated expression of Ptch1 in HCC tissues was significantly related to disease recurrence, as well as a shorter time to recurrence in patients with HCC. In vitro, NanoHHI significantly inhibited the proliferation and invasion of HCC cell lines. NanoHHI potently suppressed in vivo tumor growth of HCC xenografts in both subcutaneous and orthotopic milieus, and in contrast to sorafenib, resulted in significant attenuation of systemic metastases in the orthotopic setting. Furthermore, NanoHHI significantly decreased the population of CD133-expressing HCC cells, which have been implicated in tumor initiation and metastases.
Conclusion: Downstream Hh signaling has prognostic significance in patients with HCC as it predicts early recurrence. Gli inhibition through NanoHHI has profound tumor growth inhibition and antimetastatic effects in HCC models, which may provide a new strategy in the treatment of patients with HCC and prevention post-operative recurrence. Clin Cancer Res; 18(5); 1291–302. ©2011 AACR.
Randomized Phase II Trial of Letrozole plus Anti-MUC1 Antibody AS1402 in Hormone Receptor–Positive Locally Advanced or Metastatic Breast Cancer
Authors: Ibrahim Nuhad K. , Yariz Kemal O. , Bondarenko Ihor , Manikhas Alexei , Semiglazov Vladimir , Alyasova Anna , Komisarenko Volodymyr , Shparyk Yaroslav , Murray James Lee , Jones David , Senderovich Shai , Chau Albert , Erlandsson Fredrik , Acton Gary , Pegram Mark 
Published: August 2011

Abstract
Purpose: AS1402 is a humanized immunoglobulin G1 antibody that targets the aberrantly glycosylated antigen MUC1, which is overexpressed in 90% of breast tumors and contributes to estrogen-mediated growth and survival of breast cancer cells in vitro by modulating estrogen receptor (ER) activity. Aromatase inhibitors have been reported to enhance antibody-dependent cell-mediated cytotoxicity elicited by antibodies in vitro. We compared the outcomes of patients with breast cancer treated with letrozole with or without AS1402.
Experimental Design: The study population included 110 patients with locally advanced or metastatic hormone receptor–positive breast cancer randomized to receive 2.5 mg letrozole only once daily or with a weekly 9 mg/kg AS1402 infusion. The primary endpoint was overall response rate. Secondary endpoints included progression-free survival, time to progression, and safety. AS1402 exposure and influence of allotypes of FcγRIIIa, FcγRIIa, and MUC1 were evaluated.
Results: The study was stopped early because of a trend toward worse response rates and a higher rate of early disease progression in the AS1402 + letrozole arm. Final analysis revealed no significant difference in efficacy between the study arms. Evaluated gene polymorphisms did not define patient subgroups with improved outcomes. Addition of AS1402 to letrozole was associated with manageable toxicity.
Conclusions: Because adding AS1402 to letrozole did not improve outcomes compared with letrozole only, blocking ER may be a better strategy for harnessing MUC1 modulation of the ER to a clinical advantage. FcγRIIIa, FcγRIIa, and MUC1 allotype did not predict outcome for patients treated with letrozole with or without AS1402. Clin Cancer Res; 17(21); 6822–30. ©2011 AACR.