bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cbl‐b enhances Runx2 protein stability and augments osteocalcin promoter activity in osteoblastic cell lines
Authors: Salingcarnboriboon R. Ampornaramveth , Pavasant P. , Noda M. 
Published: May 2010

AbstractCbl‐b is a member of Cbl family of E3 ubiquitin (Ub) ligase. Besides the important role in ubiquitination process, other members of Cbl family have been suggested to show non‐ubiquitination‐related function in regulation of osteoblastic differentiation. However, the role of Cbl‐b in regulation of osteoblastic function has not been known yet. To elucidate the role of Cbl‐b in regulation of osteoblastic function, we examined its effects on Runx2, a master gene of osteoblastic differentiation. We co‐expressed Cbl‐b and Runx2 in osteoblastic cell lines and tested their effects on osteocalcin promoter activity together with the expression of Runx2 and its downstream genes. Luciferase assay demonstrated that Cbl‐b synergistically enhances osteocalcin promoter activity in conjunction with the effect on Runx2. Co‐transfection of Cbl‐b and Runx2 further upregulated Runx2 protein levels without any alteration in Runx2 mRNA expression. The upregulation of Runx2 protein by Cbl‐b was inhibited by the treatment with lactacystin, a specific inhibitor of the 26S proteasome. These results indicated that Cbl‐b would control Runx2 protein levels at the post‐translational event. Moreover, the upregulation of downstream genes of Runx2 such as osteocalcin and alkaline phosphatase mRNA was also observed. These data propose the involvement of Cbl‐b in the regulation of osteoblast‐related genes expression. J. Cell. Physiol. 224: 743–747, 2010. © 2010 Wiley‐Liss, Inc.
MED and PSACH COMP mutations affect chondrogenesis in chicken limb bud micromass cultures
Authors: Roman‐Blas J. , Dion A.S. , Seghatoleslami M.R. , Giunta K. , Oca P. , Jimenez S.A. , Williams C.J. 
Published: May 2010

AbstractMutations in cartilage oligomeric matrix protein (COMP) cause pseudoachondroplasia (PSACH) and multiple epiphyseal dysplasia (MED). We studied the effects of over‐expression of wild type and mutant COMP on early stages of chondrogenesis in chicken limb bud micromass cultures. Cells were transduced with RCAS virus harboring wild type or mutant (C328R, PSACH; T585R, MED) COMP cDNAs and cultured for 3, 4, and 5 days. The effect of COMP constructs on chondrogenesis was assessed by analyzing mRNA and protein expression of several COMP binding partners. Cell viability was assayed, and evaluation of apoptosis was performed by monitoring caspase 3 processing. Over‐expression of COMP, and especially expression of COMP mutants, had a profound affect on the expression of syndecan 3 and tenascin C, early markers of chondrogenesis. Over‐expression of COMP did not affect levels of type II collagen or matrilin‐3; however, there were increases in type IX collagen expression and sulfated proteoglycan synthesis, particularly at day 5 of harvest. In contrast to cells over‐expressing COMP, cells with mutant COMP showed reduction in type IX collagen expression and increased matrilin 3 expression. Finally, reduction in cell viability, and increased activity of caspase 3, at days 4 and 5, were observed in cultures expressing either wild type or mutant COMP. MED, and PSACH mutations, despite displaying phenotypic differences, demonstrated only subtle differences in their cellular viability and mRNA and protein expression of components of the extracellular matrix, including those that interact with COMP. These results suggest that COMP mutations, by disrupting normal interactions between COMP and its binding partners, significantly affect chondrogenesis. J. Cell. Physiol. 224: 817–826, 2010. © 2010 Wiley‐Liss, Inc.
PED interacts with Rac1 and regulates cell migration/invasion processes in human non‐small cell lung cancer cells
Authors: Zanca Ciro , Cozzolino Flora , Quintavalle Cristina , Di Costanzo Stefania , Ricci‐Vitiani Lucia , Santoriello Margherita , Monti Maria , Pucci Piero , Condorelli Gerolama 
Published: May 2010

AbstractPED (phosphoprotein enriched in diabetes) is a 15 kDa protein involved in many cellular pathways and human diseases including type II diabetes and cancer. We recently reported that PED is overexpressed in human cancers and mediates resistance to induced apoptosis. To better understand its role in cancer, we investigated on PED interactome in non‐small cell lung cancer (NSCLC). By the Tandem Affinity Purification (TAP), we identified and characterized among others, Rac1, a member of mammalian Rho GTPase protein family, as PED‐interacting protein. In this study we show that PED coadiuvates Rac1 activation by regulating AKT mediated Rac1‐Ser71 phosphorylation. Furthermore, we show that the expression of a constitutively active Rac, affected PED‐Ser104 phosphorylation, which is important for PED‐regulated ERK 1/2 nuclear localization. Through specific Rac1‐siRNA or its pharmacological inhibition, we demonstrate that PED augments migration and invasion in a Rac1‐dependent manner in NSCLC. In conclusion, we show for the first time that PED and Rac1 interact and that this interaction modulates cell migration/invasion processes in cancer cells through ERK1/2 pathway. J. Cell. Physiol. 225: 63–72, 2010. © 2010 Wiley‐Liss, Inc.
TNFalpha up‐regulates SLUG via the NF‐kappaB/HIF1alpha axis, which imparts breast cancer cells with a stem cell‐like phenotype
Authors: Storci Gianluca , Sansone Pasquale , Mari Sara , D'Uva Gabriele , Tavolari Simona , Guarnieri Tiziana , Taffurelli Mario , Ceccarelli Claudio , Santini Donatella , Chieco Pasquale , Marcu Kenneth B. , Bonafè Massimiliano 
Published: May 2010

AbstractExtracellular and intracellular mediators of inflammation, such as tumor necrosis factor alpha (TNFα) and NF‐kappaB (NF‐κB), play major roles in breast cancer pathogenesis, progression and relapse. SLUG, a mediator of the epithelial–mesenchymal transition process, is over‐expressed in CD44+/CD24− tumor initiating breast cancer cells and in basal‐like carcinoma, a subtype of aggressive breast cancer endowed with a stem cell‐like gene expression profile. Cancer stem cells also over‐express members of the pro‐inflammatory NF‐κB network, but their functional relationship with SLUG expression in breast cancer cells remains unclear. Here, we show that TNFα treatment of human breast cancer cells up‐regulates SLUG with a dependency on canonical NF‐κB/HIF1α signaling, which is strongly enhanced by p53 inactivation. Moreover, SLUG up‐regulation engenders breast cancer cells with stem cell‐like properties including enhanced expression of CD44 and Jagged‐1 in conjunction with estrogen receptor alpha down‐regulation, growth as mammospheres, and extracellular matrix invasiveness. Our results reveal a molecular mechanism whereby TNFα, a major pro‐inflammatory cytokine, imparts breast cancer cells with stem cell‐like features, which are connected to increased tumor aggressiveness. J. Cell. Physiol. 225: 682–691, 2010. © 2010 Wiley‐Liss, Inc.
Synthesis and visible‐light‐derived photocatalysis of titania nanosphere stacking layers prepared by chemical vapor deposition
Authors: Hsieh Chien‐Te , Lai Meng‐Hsuan , Pan Ching 
Published: May 2010

AbstractBACKGROUND: In this study, visible‐light‐derived photocatalytic activity of metal‐doped titanium dioxide nanosphere (TS) stacking layers, prepared by chemical vapor deposition (CVD), was investigated. The as‐grown TS spheres, having an average diameter of 100–300 nm, formed a layer‐by‐layer stacking layer on a glass substrate. The crystalline structures of the TS samples were of anatase‐type.RESULTS: Ultraviolet (UV) absorption confirmed that metallic doping (i.e. Co and Ni) shifted the light absorption of the spheres to the visible‐light region. With increasing dopant density, the optical band gap of the nanospheres became narrower, e.g. the smallest band gap of Co‐doped TS was 2.61 eV. Both Ni‐ and Co‐doped TS catalysts showed a photocatalytic capability in decomposing organic dyes under visible irradiation. In comparison, Co‐doped TiO2 catalyst not only displays the adsorption capacity, but also the photocatalytic activity higher than the N‐doped TiO2 catalyst.CONCLUSION: This result can be attributed to the fact that the narrower band gap easily generates electron–hole pairs over the TS catalysts under visible irradiation, thus, leading to the higher photocatalytic activity. Accordingly, this study shed some light on the one‐step efficient CVD approach to synthesize metal‐doped TS catalysts for decomposing dye compounds in aqueous solution. Copyright © 2010 Society of Chemical Industry
Ultrastructural observations on antennal sensilla of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae)
Authors: Sun Xiao , Wang Man‐Qun , Zhang Guoan 
Published: May 2010

AbstractThe morphology and ultrastructure of the antennal sensilla of Cnaphalocrocis medinalis (Lepidoptera: Pyralidae) was studied by scanning and transmission electron microscopic techniques. Eight morphological types of sensillum were recorded in both sexes: sensilla trichodea (S. trichodea), sensilla basiconc (S. basiconc), sensilla coeloconica (S. coeloconica), sensilla styloconica (S. styloconica), sensilla squamous (S. squamous), sensilla auricillica (S. auricillica), Böhm bristles (B. bristles), and sensilla cavity (S. cavity). S. trichodea were the most abundant sensilla and were distributed over the entire antennal surface. Four different types of S. trichodea and S. basiconc were observed. The number of S. basiconc and S. coeloconica of males were greater than those of females of C. medinalis. S. squamous formed on the dorsal part of the antenna, as a cluster in females and as in a line in males. Higher magnification revealed that S. basiconc had an olfactory function, while the character of longer length of these sensilla suggested that they also played a role in sensing mechanical or other chemical stimuli. Transmission electron microscopy (TEM) of S. squamous revealed nonporous walls suggesting a non‐olfactory function. S. coeloconica, S. styloconica, and S. cavity may be involved in the perception of humidity, temperature, heat, and CO2. Because of their particular location, we infer that B. bristles may function in sensing the position and movements of the antennae, while the function of S. auricillica is as yet unknown. The results obtained provide direct morphological evidence that antennae possess structures that can play a role in finding mates and locating host plants. Microsc. Res. Tech. 74:113‐121, 2011. © 2010 Wiley‐Liss, Inc.
Distribution of nestin protein: Immunohistochemical study in enteric plexus of rat duodenum
Authors: Carmona I. Cantarero , Luesma Bartolomé M.J. , Lavoie‐Gagnon C. , Junquera Escribano C. 
Published: May 2010

AbstractThe intermediate nestin filament is expressed in neural stem cells, neuroectodermal tumors and various adult tissues under situations that reproduce developmental phases, e.g., physiological renewal of certain cell types, tissue regeneration, and healing or revascularization. In the human gastrointestinal tract, nestin has been reported in glial cells and interstitial cells of Cajal. We examined by immunohistochemistry the appearance and distribution of nestin protein in enteric ganglia of rat duodenum. Through the myenteric and submucosal plexuses, a high number of nestin‐positive cells were visualized in this specie. The nestin‐positive cells were smaller and more numerous than enteric neurons. They were present both within and around ganglia. The results of this study suggest that the rat enteric glial cells (EGCs) are rich in nestin, a protein usually associated with dividing or migrating cells and the dynamic reorganization of nestin filaments during the cell cycle. EGCs could function as enteric stem cells. Microsc. Res. Tech. 74:148–152, 2011. © 2010 Wiley‐Liss, Inc.
Modeling and optimization of microwave structures using quick space mapping with variable weight coefficients
Authors: Koziel Slawomir , Bandler John W. 
Published: May 2010

AbstractWe describe an improvement of a recent space mapping (SM) modeling approach that uses variable weight coefficients (SM‐VWC). Our modification alleviates the main drawback of SM‐VWC: the computational overhead related to a separate parameter extraction required for each evaluation of the surrogate model. In our new procedure, the output SM parameters of the surrogate model are obtained by solving a linear regression problem instead of being determined in the nonlinear parameter extraction process. This dramatically reduces the evaluation time of the surrogate model. Moreover, the modeling accuracy of the modified technique is even better than the accuracy of the original SM‐VWC approach, which is because—in a practical implementation—the original SM‐VWC technique reduces the computational cost of the parameter extraction at the expense of its quality. Examples demonstrate the robustness of our approach. Applications of the quick SM‐VWC for the parametric design optimization are also presented. Copyright © 2010 John Wiley & Sons, Ltd.
Role of adenosine kinase in cochlear development and response to noise
Authors: Vlajkovic Srdjan M. , Guo Cindy X. , Dharmawardana Nuwan , Wong Ann Chi Yan , Boison Detlev , Housley Gary D. , Thorne Peter R. 
Published: May 2010

AbstractAdenosine signalling has an important role in cochlear protection from oxidative stress. In most tissues, intracellular adenosine kinase (ADK) is the primary route of adenosine metabolism and the key regulator of intracellular and extracellular adenosine levels. The present study provides the first evidence for ADK distribution in the adult and developing rat cochlea. In the adult cochlea, ADK was localized to the nuclear or perinuclear region of spiral ganglion neurons, lateral wall tissues, and epithelial cells lining scala media. In the developing cochlea, ADK was strongly expressed in multiple cell types at birth and reached its peak level of expression at postnatal day 21 (P21). Ontogenetic changes in ADK expression were evident in the spiral ganglion, organ of Corti, and stria vascularis. In the spiral ganglion, ADK showed a shift from predominantly satellite cell immunolabelling at P1 to neuronal expression from P14 onward. In contrast to the role of ADK in various aspects of cochlear development, the ADK contribution to the cochlear response to noise stress was less obvious. Transcript and protein levels of ADK were unaltered in the cochlea exposed to broadband noise (90–110 dBSPL, 24 hr), and the selective inhibition of ADK in the cochlea with ABT‐702 failed to restore hearing thresholds after exposure to traumatic noise. This study indicates that ADK is involved in purine salvage pathways for nucleotide synthesis in the adult cochlea, but its role in the regulation of adenosine signalling under physiological and pathological conditions has yet to be established. © 2010 Wiley‐Liss, Inc.
Phosphorus uptake by cowpea plants from sparingly available or soluble sources as affected by nitrogen form and arbuscular‐mycorrhiza‐fungal inoculation
Authors: Ngwene Benard , George Eckhard , Claussen Winfried , Neumann Elke 
Published: May 2010

AbstractIn most plant species, nutrient uptake is facilitated upon root association with symbiotic arbuscular mycorrhizal (AM) fungi. The aim of the present experiment was to test how the form in which nitrogen (N) is supplied to the growth medium affects substrate pH, AM development, and contribution of the symbiosis to phosphorus (P) uptake from sparingly available or soluble resources. Cowpea (Vigna unguiculata L. Walp) plants inoculated or noninoculated with AM fungi (Glomus sp.) were grown in pots with a sand substrate supplied with nutrient solution. The nutrient solution was prepared either with a high or a low concentration of soluble P, and NO$ _3^- $‐N : NH$ _4^+ $‐N ratios of 9:1 or 5:5. The substrate supplied with low‐P nutrient solution was either or not additionally amended with ground rock phosphate. Despite a high level of root colonization, AM fungi used in the present study did not appear to increase plant availability of rock phosphate. It cannot be excluded that the ability of AM root systems to acquire P from sparingly available resources differs depending on the plant and fungal genotypes or environmental conditions. The absence from the growth substrate of P‐solubilizing microorganisms able to associate with AM mycelia might also have been a reason for this observation in our study. Increased supply of NH$ _4^+ $ relative to NO$ _3^- $ improved plant P availability from rock phosphate, but also had a negative effect on the extent of AM‐fungal root colonization, irrespective of the plant P‐nutritional status. Whether increasing levels of NH$ _4^+ $ can also negatively affect the functioning of the AM symbiosis in terms of plant element uptake, pathogen protection or soil‐structure stabilization deserves further investigation.
Distant nodal metastases from intrathoracic esophageal squamous cell carcinoma: Characteristics of long‐term survivors after chemoradiotherapy
Authors: Chao Yin‐Kai , Wu Yi‐Cheng , Liu Yun‐Hen , Tseng Chen‐Kan , Chang Hsien‐Kun , Hsieh Ming‐Ju , Chu Yen , Liu Hui‐Ping 
Published: May 2010

AbstractBackgroundNon‐regional lymph node metastasis in intrathoracic esophageal cancer is classified as M1 lesion with poor prognosis following surgery alone. We studied the controversial question of whether chemoradiotherapy (CRT) improves survival of these patients.MethodsA cohort of patients with clinically overt nodal M1 disease, which could be encompassed by a tolerable radiation therapy port, was selected from the database of the Chang Gung Memorial Hospital.ResultsFrom 1994 to 2005, 54 nodal stage IV intrathoracic esophageal squamous cell carcinoma (SCC) patients received neoadjuvant CRT. Significant response occurred in 24 patients. Scheduled esophagectomy was performed in 26 patients. The 3‐year overall survival (OS) and disease‐free survival (DFS) for the whole group were 27% (median: 14.2 months) and 22% (median: 14.7 months), respectively. Multivariate analysis identified pretherapy lymph nodes classified as M1a and R0 resection after CRT as independent favorable prognosticators. Median survival reached 36.9 months in the pretherapy M1a subgroup as opposed to 12.5 months in the M1b subgroup (3‐year‐DFS: 40% vs. 10%, P = 0.0117). Scheduled surgery after CRT benefits only after R0 resection (3‐year‐DFS: 36%, median survival: 45 months). The group with incomplete resection had a high surgical risk and dismal survival compared to the non‐surgery group (3‐year‐DFS: 0% vs. 9%, 9.5 vs. 10.5 months).ConclusionsPretherapy M1a disease had a significantly better survival than nodal M1b disease after CRT in SCC. Aggressive surgical treatment after CRT is reserved for cases when complete resection is anticipated. J. Surg. Oncol. 2010;102:158–162. © 2010 Wiley‐Liss, Inc.
Ten years later: A single hospital experience with malignancy in HIV/AIDS
Authors: Reed Mary , Cosgrove John M. , Cindrich Richard , Parithivel Vellore S. , GAD Youhanna , Bangalore Madan , Uzor Robert , Kalim Jawaid , Segura Raymundo , Albu Eugene 
Published: May 2010

AbstractBackground and ObjectiveWe present our experience in the era of HAART with 5,112 patients having HIV infection or AIDS, treated between 2002 and 2006 in our hospital, 182 of whom had malignancies (3.56%). We compared our findings to those from a similar cohort of patients studied 10 years earlier.MethodsThe charts were reviewed and data was electronically collected as in our 1993–1998 study. Similar statistical analyses were performed in both studies and the results were compared.ResultsFor the current study the average patient age increased by 9 years. A decrease in AIDS‐defining cancers (ADC), from 63.6% to 37.3% and a higher incidence of non‐AIDS‐defining cancers (NADC), 62.7 as opposed to 37.9% was found. No decrease in the incidence of non‐Hodgkin's B cell lymphoma (NHL) was noted. There was an increase in the number of opportunistic infections notably hepatitis C virus (HCV) and hepatitis B virus (HBV).ConclusionsHIV/AIDS patients on HAART are older, have lower rates of AIDS related Kaposi's sarcoma and a higher incidence of NADCs than did patients in the early HAART era. No decrease in the proportion of NHL was observed. J. Surg. Oncol. 2010;102:282–286. © 2010 Wiley‐Liss, Inc.
[18F]‐fluorodeoxyglucose‐positron emission tomography for the assessment of histopathologic response and prognosis after completion of neoadjuvant chemotherapy in gastric cancer
Authors: Vallböhmer D. , Hölscher A.H. , Schneider P.M. , Schmidt M. , Dietlein M. , Bollschweiler E. , Baldus S. , Alakus H. , Brabender J. , Metzger R. , Mönig S. P. 
Published: May 2010

AbstractBackground and ObjectivesNeoadjuvant chemotherapy is applied to improve the prognosis of patients with advanced gastric cancer. However, only a major histopathological response will provide a benefit. Recent studies suggest that [18F]‐fluorodeoxyglucose‐positron‐emission‐tomography (FDG‐PET) correlates with response and survival in patients with gastroesophageal adenocarcinomas undergoing neoadjuvant chemotherapy. We evaluated the potential of FDG‐PET for the assessment of response and prognosis in the multimodality treatment of gastric cancer.MethodsStudy patients were recruited from a prospective observation trial. Forty two patients with advanced gastric cancer received neoadjuvant chemotherapy and subsequently 40 patients underwent standardized gastrectomy (2 patients with tumor progression had therapy limited to palliative chemotherapy without surgery). Histomorphologic regression was defined as major response when resected specimens contained <10% vital tumor cells. FDG‐PET was performed before and 2 weeks after the end of neoadjuvant chemotherapy with assessment of the intratumoral FDG‐uptake [pre‐treatment standardized uptake value (SUV1); post‐treatment SUV (SUV2); percentage change (SUVΔ%)].ResultsHistomorphological tumor regression was confirmed as a prognostic factor (P = 0.039). No significant correlations between SUV1, SUV2, or SUVΔ% and response or prognosis were found.ConclusionFDG‐PET seems not to be an imaging system that effectively characterizes major/minor response and survival in patients with gastric cancer following multimodality treatment. J. Surg. Oncol. 2010;102:135–140. © 2010 Wiley‐Liss, Inc.
Magnesium oxide microspheres as a novel solid‐phase extraction sorbent for the determination of benzo[a]pyrene in environmental water samples
Authors: Jin Jing , Zhang Zhiping , Wang Jincheng , Qi Peipei , Chen Jiping 
Published: May 2010

AbstractMagnesium oxide microspheres were developed as a novel SPE sorbent for the determination of benzo[a]pyrene (BaP), one of the most potent carcinogenic agents, in environmental water samples. The parameters controlling the extraction efficiency, such as elution volume, flow rate, pH values, and breakthrough volume, were investigated in detail. Considering the facile preparation and satisfying recovery, a corresponding analytical method has been developed to determine the concentration of BaP in real tap water, river water, and seawater. The recoveries for the spiked BaP were excellent (94–101%).
Simultaneous determination of substrate and product in the process of preparation of valienamine by capillary zone electrophoresis
Authors: Wei Xiao‐Dong , Zhao Wen‐Jie , Gu Min , Zhao Bo , Yao Rong‐Ying 
Published: May 2010

AbstractA simple and rapid CZE method was established for the simultaneous determination of valienamine, acarbose and validamycin A, using a 20‐kV CZE with the detection wavelength of 193 nm and 50 mM phosphoric acid–20 mM Tris (pH 5.3) as a running buffer. The calibration curves of valienamine, acarbose, and validamycin A showed a good linear relationship at a concentration range of 5–1000 μg/mL. The detection limits of valienamine, acarbose, and validamycin A were 0.3, 0.6, and 0.6 μg/mL, respectively, and the average recoveries of each of the above were 99.9, 99.5, and 100.3%. The method has been successfully applied for simultaneous determination of substrate and product in the process of preparation of valienamine.