bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of untyped SNPs: maximum likelihood and imputation methods
Authors: Hu Y.J. , Lin D.Y. 
Published: November 2010

AbstractAnalysis of untyped single nucleotide polymorphisms (SNPs) can facilitate the localization of disease‐causing variants and permit meta‐analysis of association studies with different genotyping platforms. We present two approaches for using the linkage disequilibrium structure of an external reference panel to infer the unknown value of an untyped SNP from the observed genotypes of typed SNPs. The maximum‐likelihood approach integrates the prediction of untyped genotypes and estimation of association parameters into a single framework and yields consistent and efficient estimators of genetic effects and gene‐environment interactions with proper variance estimators. The imputation approach is a two‐stage strategy, which first imputes the untyped genotypes by either the most likely genotypes or the expected genotype counts and then uses the imputed values in a downstream association analysis. The latter approach has proper control of type I error in single‐SNP tests with possible covariate adjustments even when the reference panel is misspecified; however, type I error may not be properly controlled in testing multiple‐SNP effects or gene‐environment interactions. In general, imputation yields biased estimators of genetic effects and gene‐environment interactions, and the variances are underestimated. We conduct extensive simulation studies to compare the bias, type I error, power, and confidence interval coverage between the maximum likelihood and imputation approaches in the analysis of single‐SNP effects, multiple‐SNP effects, and gene‐environment interactions under cross‐sectional and case‐control designs. In addition, we provide an illustration with genome‐wide data from the Wellcome Trust Case‐Control Consortium (WTCCC) [2007]. Genet. Epidemiol. 34:803–815, 2010. © 2010 Wiley‐Liss, Inc.
Preferences for new and existing contraceptive products
Authors: Fiebig Denzil G. , Knox Stephanie , Viney Rosalie , Haas Marion , Street Deborah J. 
Published: November 2010

AbstractNew contraceptive methods provide greater choice in terms of effectiveness, management of side‐effects, convenience and frequency of administration and flexibility, but make the decisions about contraception more complex. There are limited data on the factors that determine women's choices among these alternatives, to inform providers about the factors which are most important to women, or to predict uptake of new products. This paper reports on a choice experiment designed to elicit women's preferences in relation to prescribed contraception and to forecast the impact of the introduction of two new products into the Australian market. A generalised multinomial logit model is estimated and used in the simulation exercise. The model forecasts that the hormonal patch would be well received among women, achieving a greater market share than current non‐pill products, but the vaginal ring would have limited appeal. Copyright © 2011 John Wiley & Sons, Ltd.
Scope insensitivity in contingent valuation studies of health care services: should we ask twice?
Authors: Gyrd‐Hansen D. , Kjær T. , Nielsen J. S. 
Published: November 2010

SUMMARYThe main purpose of the present study was to test for outcome scope insensitivity. Respondents were initially asked to value one of two severe health states by way of a time‐trade‐off (TTO) exercise. Subsequent to the TTO exercise all respondents were asked to value an intervention, which offered a reduction in risk of falling into the health state they had evaluated. All respondents were subsequent to this initial CV exercise asked to value the same risk reduction, but in this case the outcome was death. Although our study passes the internal scope test, there is not a high degree of sensitivity to outcome. As many as 68% of respondents stated an identical maximum WTP in first and second CV valuation exercise implying that they value the interventions equally despite the fact that the health state presented in the initial CV question was deemed far better than death according to the TTO responses given by the same respondents. In contrast, the external scope test (comparison of response to initial CV across study arms) fared much better. Copyright © 2010 John Wiley & Sons, Ltd.
Labor adjustment in the Japanese health care industry: some empirical evidence
Authors: Inagaki Kazuyuki 
Published: November 2010

SUMMARYThis paper examines the labor adjustment costs in the health care industry. Using Japanese data, we find that the cost of hiring new health care workers is the largest component of labor adjustment costs in the health care industry. Hence, it is difficult for employers in this industry to immediately increase the number of workers since this employment adjustment is extremely expensive. Copyright © 2010 John Wiley & Sons, Ltd.
The human factors evaluation program of a control room: The French EPR approach
Authors: Labarthe Jean‐Paul , Garza Cecilia De la 
Published: November 2010

AbstractThe aim of this article is to explain how a human factors evaluation program has been constructed over the last 10 years in the French energy company (Electricité de France [EDF]). This program evaluation is conducted by a human factors team from EDF Research and Development (R&D), specialized in ergonomics and involved in the EPR Project for the operating means and the control room design. Of course, other kinds of experts have been associated, such as designers, future end users, and current end users of control rooms. This article is focused on the methodology developed for this evaluation program. The test protocols, the characteristics of the sample, the data collection, and the data analysis are described to understand the qualitative approach performed and their interest for the French EPR. An example of an evaluation step, based on the simulation of the future activity, is described and discussed. © 2010 Wiley Periodicals, Inc.
Reducing burnout in call centers through HR practices
Authors: Castanheira Filipa , Chambel Maria José 
Published: November 2010

AbstractPrevious research on call centers has demonstrated that human resource (HR) practices can be related to employee stress; however, these studies did not examine the linking mechanisms underlying these associations. Using the job demands–control (JD‐C) model as a theoretical framework, we examine perceived job demands (namely, emotional dissonance and quantitative demands) and autonomy as potential mediators in the relationship between HR systems and burnout (exhaustion and cynicism). We distinguish between HR control systems, which include performance monitoring practices, and HR involvement systems, which include training, participation, and performance‐related pay. This study samples 811 employees working in 11 call centers. Our findings support the idea that HR systems can help reduce burnout in call centers by verifying that HR control systems associated with more emotional dissonance and less autonomy increase burnout. On the other hand, an HR involvement system decreases workers' burnout because it alleviates the job demands of emotional dissonance and quantitative demands. This study fills a gap in the literature between HR systems and burnout by demonstrating the role job demands and autonomy play in explaining how HR systems improve or decrease workers' exhaustion and cynicism. © 2010 Wiley Periodicals, Inc.
Serum thyroglobulin, a biomarker for iodine deficiency, is not associated with increased risk of upper gastrointestinal cancers in a large Chinese cohort
Authors: Lin Shih‐Wen , Fan Jin‐Hu , Dawsey Sanford M. , Taylor Philip R. , Qiao You‐Lin , Abnet Christian C. 
Published: November 2010

AbstractIodine concentrates in gastric tissue and may act as an antioxidant for the stomach. We previously showed that self‐reported goiter was associated with significantly increased risk of gastric noncardia adenocarcinoma (GNCA) and nonsignificantly increased risks of gastric cardia adenocarcinoma (GCA) and esophageal squamous cell carcinoma (ESCC) in a prospective case–cohort study in a high‐risk population in China. Negatively correlated with iodine levels, serum thyroglobulin (Tg) is a more sensitive biomarker of iodine deficiency than goiter. Our study aimed to determine whether baseline serum Tg was also associated with development of GNCA, GCA and ESCC in the same cohort, the Linxian General Population Nutrition Intervention Trial. Sera from ∼200 subjects of each case type and 400 noncases were tested for serum Tg concentration using appropriate assays. Tg was modeled as sex‐ and assay‐specific quartiles in Cox regression models adjusted for age, smoking, alcohol, Helicobacter pylori status, pepsinogens I/II ratio, family history and commune of residence. In the final combined analysis, participants in the highest quartile of serum Tg, compared to those in the lowest quartile, had adjusted hazard ratios of 0.88 (95% confidence interval 0.50–1.52), 1.14 (0.63–2.05) and 0.78 (0.47–1.31) for GNCA, GCA and ESCC, respectively. Using serum Tg, a sensitive biomarker of iodine deficiency, we found no association between serum Tg concentrations and risk of these upper gastrointestinal (UGI) cancers in the study population. Our results do not support the hypothesis that iodine deficiency, as assessed by serum Tg, is associated with an increased risk of UGI cancers.
Finite element analysis performed on radius and tibia HR-pQCT images and fragility fractures at all sites in men
Authors: Vilayphiou Nicolas , Boutroy Stephanie , Szulc Pawel , van Rietbergen Bert , Munoz Francoise , Delmas Pierre D , Chapurlat Roland 
Published: November 2010

Abstract
Few studies have investigated bone microarchitecture and biomechanical properties in men. This study assessed in vivo both aspects in a population of 185 men (aged 71 ± 10 years) with prevalent fragility fractures, compared to 185 controls matched for age, height, and weight, from the Structure of the Aging Men's Bones (STRAMBO) cohort.
In this case-control study, areal BMD (aBMD) was measured by DXA, bone microarchitecture was assessed by high resolution (HR)-pQCT, and finite element (µFE) analysis was based on HR-pQCT images of distal radius and tibia. A principal component (PC) analysis (PCA) was used to study the association of synthetic PCs with fracture by computing their odds ratio (OR [95%CI]) per SD change. Specific associations with vertebral fracture (n = 100), and nonvertebral fracture (n = 85) were also computed.
At both sites, areal and volumetric BMD, cortical thickness and trabecular number, separation, and distribution were significantly worse in cases than in controls, with differences ranging from −6% to 15%. µFE-derived stiffness and failure load were 8% to 9% lower in fractures (p &lt; .01). No difference in load distribution was found between the two groups. After adjustment for aBMD, only differences of µFE-derived stresses, stiffness, and failure load at the tibia remained significant (p &lt; .05).
PCA resulted in defining 4 independent PCs, explaining 83% of the total variability of bone characteristics. Nonvertebral fractures were associated with PC1, reflecting bone quantity and strength at the radius (tibia) with OR = 1.64 [1.27–2.12] (2.21 [1.60–3.04]), and with PC2, defined by trabecular microarchitecture, with OR = 1.27 [1.00–1.61]. Severe vertebral fractures were associated with PC1, with OR = 1.56 [1.16–2.09] (2.21 [1.59–3.07]), and with PC2, with OR = 1.55 [1.17–2.06] (1.45 [1.06–1.98]).
In conclusion, microarchitecture and biomechanical properties derived from µFE were associated with all types of fractures in men, showing that radius and tibia mechanical properties were relatively representative of distant bone site properties. © 2011 American Society for Bone and Mineral Research.
Effects of 25-hydroxyvitamin D3 on proliferation and osteoblast differentiation of human marrow stromal cells require CYP27B1/1α-hydroxylase
Authors: Geng Shuo , Zhou Shuanhu , Glowacki Julie 
Published: November 2010

Abstract
1,25-Dihydroxyvitamin D3 [1,25(OH)2D3] has many noncalcemic actions that rest on inhibition of proliferation and promotion of differentiation in malignant and normal cell types. 1,25(OH)2D3 stimulates osteoblast differentiation of human marrow stromal cells (hMSCs), but little is known about the effects of 25-hydroxyvitamin D3 [25(OH)D3] on these cells. Recent evidence shows that hMSCs participate in vitamin D metabolism and can activate 25(OH)D3 by CYP27B1/1α-hydroxylase. These studies test the hypothesis that antiproliferative and prodifferentiation effects of 25(OH)D3 in hMSCs depend on CYP27B1. We studied hMSCs that constitutively express high (hMSCshi-1α) or low (hMSCslo-1α) levels of CYP27B1 with equivalent expression of CYP24A1 and vitamin D receptor. In hMSCshi-1α, 25(OH)D3 reduced proliferation, downregulated proliferating cell nuclear antigen (PCNA), upregulated p21Waf1/Cip1, and decreased cyclin D1. Unlike 1,25(OH)2D3, the antiapoptotic effects of 25(OH)D3 on Bax and Bcl-2 were blocked by the P450 inhibitor ketoconazole. The antiproliferative effects of 25(OH)D3 in hMSCshi-1α and of 1,25(OH)2D3 in both samples of hMSCs were explained by cell cycle arrest, not by increased apoptosis. Stimulation of osteoblast differentiation in hMSCshi-1α by 25(OH)D3 was prevented by ketoconazole and upon transfection with CYP27B1 siRNA. These data indicate that CYP27B1 is required for 25(OH)D3's action in hMSCs. Three lines of evidence indicate that CYP27B1 is required for the antiproliferative and prodifferentiation effects of 25(OH)D3 on hMSCs: Those effects were not seen (1) in hMSCs with low constitutive expression of CYP27B1, (2) in hMSCs treated with ketoconazole, and (3) in hMSCs in which CYP27B1 expression was silenced. Osteoblast differentiation and skeletal homeostasis may be regulated by autocrine/paracrine actions of 25(OH)D3 in hMSCs. © 2011 American Society for Bone and Mineral Research.
RETRACTED: Protective effects of N‐acetyl cysteine on lipid peroxide metabolism on isoproterenol‐induced myocardial infarcted rats
Authors: Nagoor Meeran Mohamed Fizur , Mainzen Prince Ponnian Stanely 
Published: November 2010

AbstractThe present study was designed to evaluate the preventive effects of N‐acetyl cysteine on lipid peroxide metabolism in isoproterenol (ISO) induced myocardial infarcted rats. Male albino Wistar rats were pretreated with N‐acetyl cysteine (5 and 10 mg/kg) daily for a period of 14 days. After the pretreatment period, ISO (100 mg/kg) was subcutaneously injected to rats twice at an interval of 24 h. Increased activities of serum creatine kinase, creatine kinase‐MB, lactate dehydrogenase, and increased intensities of serum lactate dehydrogenase‐isoenzyme bands (LDH‐1, LDH‐2) were observed in ISO‐induced rats. The heart lipid peroxidation products were significantly increased, and the antioxidant system was significantly reduced in ISO‐induced rats. Pretreatment with N‐acetyl cysteine (5 and 10 mg/kg) to ISO‐induced rats showed significant effects on all the biochemical parameters studied. Histopathological findings of the myocardium also showed the protective role of N‐acetyl cysteine in ISO‐induced rats. Furthermore, in vitro study confirmed the potent‐free radical scavenging activity of N‐acetyl cysteine. The effect at a dose of 10 mg/kg of N‐acetyl cysteine was more pronounced than the dose, 5 mg/kg. The results of our study show that N‐acetyl cysteine protects the heart against ISO‐induced myocardial infarction by its free radical scavenging effect. © 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:151–157 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20371
Silk fibroin has a protective effect against high glucose induced apoptosis in HIT‐T15 cells
Authors: Park Jun Hong , Nam YoonYi , Park So‐Young , Kim Jin‐Kyung , Choe Nong‐Hoon , Lee Jae‐Young , Oh Yang‐Seok , Suh Jun Gyo 
Published: November 2010

AbstractHigh glucose levels induce cell death in many cell types, including pancreatic β‐cells. Although protective agents against glucotoxicity have been searched for extensively, so far none have been found. In this report, we tested silk fibroin (SF) as a candidate material for antiglucotoxicity in the pancreatic β‐cell (HIT‐T15 cell) line. Approximately 50% of cells were killed after treatment with 80 mg/mL glucose. This reduction of cell number was recovered by the addition of SF at 50 mg/mL. SF treatment also decreased cellular reactive oxygen species (ROS) and increased proliferating cellular nuclear antigen (PCNA) immunoreactivity. In addition, TUNEL assays demonstrated that SF protects against glucose‐induced apoptosis of HIT‐T15 cells, suggesting that SF might protect cells from cell death by lowering cellular ROS levels. SF also induced expression of the insulin‐like growth factor‐1 (IGF‐1) gene, and IGF‐1 expression may be the cause of SF‐induced protection against glucose toxicity. Taken together, these results suggest that SF could serve as a potential therapeutic agent to treat the hyperglycemia‐induced death of pancreatic β‐cells. © 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:238–243, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20381
Cell‐type‐specific organization of nuclear DNA into structural looped domains
Authors: Trevilla‐García Claudia , Aranda‐Anzaldo Armando 
Published: November 2010

AbstractIn the interphase nucleus of metazoan cells the DNA is organized in supercoiled loops anchored to a proteinaceous substructure known as the nuclear matrix (NM). The DNA is anchored to the NM by means of non‐coding sequences of variable length known as matrix attachment regions or MARs operationally classified in structural‐constitutive, resistant to high‐salt extraction and transient‐functional, non‐resistant to high‐salt extraction. The former are also known as true loop attachment regions or LARs that determine structural DNA loops. The DNA–NM interactions define a higher order structure within the cell nucleus (NHOS). We studied in a comparative fashion the NHOS in two primary cell types from the rat: hepatocytes and naive B lymphocytes, by analyzing the topological relationships between the NM and a set of eight short gene sequences located in six separate chromosomes and as such representing a coarse‐grained, large‐scale sample of the actual organization of nuclear DNA into structural loop domains. Our results indicate that such an organization is cell‐type specific since most of the gene sequences studied showed significant differences in their relative position to the NM according to cell type. Such cell‐type specific differences in the NHOS have no obvious correlation with the tissue‐specific transcriptional activity of the corresponding genes, supporting the notion that permanent, structural DNA loops are different from transient, functional DNA loops that may be associated with transcription. J. Cell. Biochem. 112: 531–540, 2011. © 2010 Wiley‐Liss, Inc.
The procollagen type III, alpha 1 (COL3A1) gene first intron expresses poly‐A+ RNA corresponding to multiple ESTs and putative miRNAs
Authors: Sterling Kenneth M. 
Published: November 2010

AbstractThe mouse COL3A1 first intron is 9684 bp. RNA's of approximately 1.6 and 3.0 kb were detected by Northern hybridization analysis of poly‐A RNA from fetal mice and total RNA from suckling and adult mouse intestine using 32P‐labeled, anti‐sense RNA synthesized from a mouse COL3A1 first intron, 5 prime region, 5.4 kb Xba I fragment (1655–7030 bp), recombinant plasmid (pPI5.4x). Expression of the 1.6 and 3.0 kb RNA's was significantly reduced in adult mouse intestine, indicating that these RNAs are developmentally regulated. “BLAST” analysis indicated that the mouse first intron 5 prime sequence has 94–100% identity to 13 mouse ESTs. These mouse first intron EST's lie within the 5.4 Xba I fragment of the mouse COL3A1 first intron. Two of the mouse first intron EST's have significant identity to known miRNA, mature sequences, mmu‐miR‐466f‐3P, mmu‐miR‐1187, and mmu‐miR‐574‐5P as well as others. Predicted targets for mmu‐miR‐466f‐3P include COL1A1, COL19A1, COL11A2, COL4A1, and COL4A5 indicating that COL3A1 intronic miRNAs may regulate the expression of other collagen genes in development. J. Cell. Biochem. 112: 541–547, 2011. © 2010 Wiley‐Liss, Inc.
Enterovirus 71 modulates a COX‐2/PGE2/cAMP‐dependent viral replication in human neuroblastoma cells: Role of the c‐Src/EGFR/p42/p44 MAPK/CREB signaling pathway
Authors: Tung Wei‐Hsuan , Hsieh Hsi‐Lung , Lee I‐Ta , Yang Chuen‐Mao 
Published: November 2010

AbstractEnterovirus 71 (EV71) has been shown to induce cyclooxygenase‐2 (COX‐2) expression in human neuroblastoma SK‐N‐SH cells through the action of MAPKs, NF‐κB, and AP‐1. On the other hand, the transcription factor CREB has also been implicated in the expression of COX‐2 in other cell lines. Here, we report that EV71‐induced COX‐2 expression and PGE2 production were both inhibited by pretreatment with the PKA inhibitor H89 or by transfection with CREB siRNA. In addition, EV71‐induced COX‐2 expression and c‐Src/EGFR phosphorylation were both attenuated by transfection with c‐Src siRNA or pretreatment with the inhibitors of c‐Src (PP1) or EGF receptor (EGFR) (AG1478 and EGFR‐neutralizing antibody). We also observed that EV71‐induced p42/p44 MAPK phosphorylation was decreased following pretreatment with AG1478. Moreover, EV71‐induced COX‐2 expression was blocked by pretreatment with the p300 inhibitor GR343 or by transfection with p300 siRNA. Using immunoprecipitation and chromatin immunoprecipitation assays, we observed that EV71 stimulated the association of CREB and p300 with the COX‐2 promoter region. Notably, we also demonstrated that EV71‐induced COX‐2 expression and PGE2 production promoted viral replication via cAMP signaling. In summary, this study demonstrates that EV71 activates the c‐Src/EGFR/p42/p44 MAPK pathway in human SK‐N‐SH cell, which leads to the activation of CREB/p300, and stimulates COX‐2 expression and PGE2 release. J. Cell. Biochem. 112: 559–570, 2011. © 2010 Wiley‐Liss, Inc.
Phosphoproteome profiling for cold temperature perception
Authors: Park Seyeon , Jang Mi 
Published: November 2010

AbstractTemperature sensation initiates from the activation of cellular receptors when the cell is exposed to a decrease in temperature. Here, we applied a phosphoproteome profiling approach to the human lung epithelial cell line BEAS‐2B to elucidate cellular cold‐responsive processes. The primary aim of this study was to determine which intracellular changes of phosphorylation are accompanied by cold sensation. Eighteen protein spots that exhibited differentially phosphorylated changes in cells were identified. Most of the proteins that were phosphorylated after 5 or 10 min were returned to control levels after 30 or 60 min. Identified proteins were mainly RNA‐related (i.e., they were involved in RNA binding and splicing). Temperature (18 and 10°C) stimuli showed homologies that were detected for time course changes in phosphoproteome. The data indicated a time‐shift between two temperatures. The phosphorylation of putative cold responsive markers, such as ribosomal protein large P0 and heterochromatin‐associated proteins 1, were verified by Western blotting in cells transfected with TRPM8 or TRPA1. J. Cell. Biochem. 112: 633–642, 2011. © 2010 Wiley‐Liss, Inc.