bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interleukin-6 Regulates Androgen Synthesis in Prostate Cancer Cells
Authors: Chun Jae Yeon , Nadiminty Nagalakshmi , Dutt Smitha , Lou Wei , Yang Joy C. , Kung Hsing-Jien , Evans Christopher P. , Gao Allen C. 
Published: July 2009

Abstract
Purpose: The standard systemic treatment for prostate cancer patients is androgen deprivation therapy. Although serum testosterone concentrations were significantly reduced after androgen deprivation therapy, levels of intraprostatic androgens are reproducibly measured at concentrations sufficient to activate androgen receptor and stimulate tumor growth, suggesting that prostate cancer cells may survive androgen deprivation therapies by increasing intracrine androgen synthesis within the prostate. However, factors that regulate de novo intracrine androgen synthesis have not been identified. Interleukin-6 (IL-6) has been implicated in the modulation of androgen receptor activation and growth and differentiation in prostate cancer. In this study, we investigate whether IL-6 regulates intraprostatic androgen synthesis in prostate cancer cells.
Experimental Design: Quantitative reverse transcription-PCR and Western blotting were done to detect expression levels of steroidogenic enzymes. AKR1C3 promoter reporter was constructed and analyzed for IL-6–mediated AKR1C3 transcriptional activity. IL-6–mediated signaling was knocked down using small interfering RNA specific to IL-6 receptor and gp130, and the effect on AKR1C3 expression was examined. Intraprostatic androgen levels in prostate cancer cells in culture and in tumors were measured by an enzyme immunoassay (Testosterone EIA kit).
Results: We found that IL-6 increases the expression of genes encoding many steroidogenic enzymes, including HSD3B2 and AKR1C3, involved in androgen biosynthesis. Down-regulation of IL-6 receptor and gp130 expression using specific small interfering RNA abolished IL-6–mediated AKR1C3 expression, suggesting that IL-6 signaling is responsible for AKR1C3 expression. IL-6 increases AKR1C3 promoter activity, indicating that the increase in IL-6–mediated AKR1C3 expression is in part at the transcriptional level. Treatment of IL-6 increased testosterone level in LNCaP cells. The tumor testosterone levels were detected at 378 pg/g in tumors generated from IL-6–overexpressing LNCaP-IL6+ cells inoculated orthotopically into the prostates of castrated male nude mice.
Conclusions: These results suggest that IL-6 increases levels of intracrine androgens through enhanced expression of genes mediating androgen metabolism in prostate cancer cells.
Regulation of Colorectal Cancer Cell Apoptosis by the n-3 Polyunsaturated Fatty Acids Docosahexaenoic and Eicosapentaenoic
Authors: Giros Anna , Grzybowski Mike , Sohn Vanessa R. , Pons Elisenda , Fernandez-Morales Jessica , Xicola Rosa M. , Sethi Puja , Grzybowski Jessica , Goel Ajay , Boland C. Richard , Gassull Miquel A. , Llor Xavier 
Published: July 2009

Abstract
Several studies have suggested that the n-3 fatty acids Docosahexaenoic (DHA) and Eicosapentaenoic (EPA) have an important protective effect on colorectal cancer, and this could be at least partly due to their proapoptotic activity. It is unclear, however, how this phenomenon is triggered and what mechanisms are implicated. Here, we show that both DHA and EPA have an important proapoptotic effect on colorectal cancer cells with different molecular phenotypes but not in noncancerous cells. Apoptosis is caspase dependent, and both intrinsic and extrinsic pathways are implicated. The dimerization of Bax and Bak, the depolarization of the mitochondrial membrane, and the subsequent release of cytochrome c and Smac/Diablo to the cytosol evidence the activation of the intrinsic pathway. The implication of the extrinsic pathway is shown by the activation of caspase-8, along with the down-regulation of FLIP. The timing of caspase-8 activation, and the oligomerization of Bid with Bax, suggest a cross-talk with the intrinsic pathway. None of the death receptors that commonly initiate the extrinsic pathway: FAS, TNF-R1, and TRAIL-R2 are found to be responsible for triggering the apoptosis cascade induced by DHA and EPA. Neither PPARγ nor cyclooxygenase-2, two likely candidates to regulate this process, play a significant role. Our findings suggest that the down-regulation of two key regulatory elements of the extrinsic and intrinsic pathways, FLIP and XIAP, respectively, is determinant in the induction of apoptosis by DHA and EPA. These fatty acids could potentially be useful adjuvant anticancer agents in combination with other chemotherapeutic elements.
A Population-Based Case-Control Study of Marijuana Use and Head and Neck Squamous Cell Carcinoma
Authors: Liang Caihua , McClean Michael D. , Marsit Carmen , Christensen Brock , Peters Edward , Nelson Heather H. , Kelsey Karl T. 
Published: July 2009

Abstract
Cannabinoids, constituents of marijuana smoke, have been recognized to have potential antitumor properties. However, the epidemiologic evidence addressing the relationship between marijuana use and the induction of head and neck squamous cell carcinoma (HNSCC) is inconsistent and conflicting.
Cases (n = 434) were patients with incident HNSCC disease from nine medical facilities in the Greater Boston, MA area between December 1999 and December 2003. Controls (n = 547) were frequency matched to cases on age (±3 years), gender, and town of residence, randomly selected from Massachusetts town books. A questionnaire was adopted to collect information on lifetime marijuana use (decade-specific exposures) and associations evaluated using unconditional logistic regression.
After adjusting for potential confounders (including smoking and alcohol drinking), 10 to 20 years of marijuana use was associated with a significantly reduced risk of HNSCC [odds ratio (OR)10-&lt;20 years versus never users, 0.38; 95% confidence interval (CI), 0.22-0.67]. Among marijuana users moderate weekly use was associated with reduced risk (OR0.5-&lt;1.5 times versus &lt;0.5 time, 0.52; 95% CI, 0.32-0.85). The magnitude of reduced risk was more pronounced for those who started use at an older age (OR15-&lt;20 years versus never users, 0.53; 95% CI, 0.30-0.95; OR≥20 years versus never users, 0.39; 95% CI, 0.17-0.90; Ptrend &lt; 0.001). These inverse associations did not depend on human papillomavirus 16 antibody status. However, for the subjects who have the same level of smoking or alcohol drinking, we observed attenuated risk of HNSCC among those who use marijuana compared with those who do not.
Our study suggests that moderate marijuana use is associated with reduced risk of HNSCC.
Flavones as Colorectal Cancer Chemopreventive Agents—Phenol-O-Methylation Enhances Efficacy
Authors: Cai Hong , Sale Stewart , Schmid Ralf , Britton Robert G. , Brown Karen , Steward William P. , Gescher Andreas J. 
Published: July 2009

Abstract
Flavonoids occur ubiquitously in plants, and some possess preclinical cancer chemopreventive activity. Little is known about molecular features that mediate chemopreventive efficacy of flavonoids. Here, three related flavones, apigenin (4′,5,7-trihydroxyflavone), tricin (4′,5,7-trihydroxy-3′,5′-dimethoxyflavone), and 3′,4′,5′,5,7-pentamethoxyflavone (PMF), were compared in terms of their effects on (a) adenoma development in ApcMin mice, a model of human gastrointestinal malignancies; (b) growth of APC10.1 mouse adenoma cells in vitro; and (c) prostaglandin E-2 generation in HCA-7 human-derived colorectal cancer cells in vitro. Life-long consumption of PMF with the diet at 0.2% reduced ApcMin mouse adenoma number and burden by 43% and 61%, respectively, whereas apigenin was inactive. Tricin has previously shown activity in this model. IC50 values for murine adenoma cell growth inhibition by PMF, tricin, and apigenin were 6, 13, and 18 μmol/L, respectively. In ApcMin mice that received flavones (0.2%) for 4 weeks, adenoma cell proliferation as reflected by Ki-67 staining was reduced by PMF and tricin, but not by apigenin. On incubation with HCA-7 cells for 6 hours, PMF reduced prostaglandin E-2 generation with an IC50 of 0.8 μmol/L, a fraction of the respective values reported for tricin or apigenin. In silico PMF docked into the cyclooxygenase active site with greater affinity than tricin or apigenin. The results suggest that the rank order of cancer chemopreventive efficacy in ApcMin mice is PMF &gt; tricin &gt; apigenin, supporting the notion that the presence of O-methyl in the flavone molecular scaffold promotes gastrointestinal cancer chemopreventive efficacy.
Dissociable Neural Patterns of Facial Identity across Changes in Viewpoint
Authors: Natu Vaidehi S. , Jiang Fang , Narvekar Abhijit , Keshvari Shaiyan , Blanz Volker , O'Toole Alice J. 
Published: July 2009

AbstractWe examined the neural response patterns for facial identity independent of viewpoint and for viewpoint independent of identity. Neural activation patterns for identity and viewpoint were collected in an fMRI experiment. Faces appeared in identity-constant blocks, with variable viewpoint, and in viewpoint-constant blocks, with variable identity. Pattern-based classifiers were used to discriminate neural response patterns for all possible pairs of identities and viewpoints. To increase the likelihood of detecting distinct neural activation patterns for identity, we tested maximally dissimilar “face”–“antiface” pairs and normal face pairs. Neural response patterns for four of six identity pairs, including the “face”–“antiface” pairs, were discriminated at levels above chance. A behavioral experiment showed accord between perceptual and neural discrimination, indicating that the classifier tapped a high-level visual identity code. Neural activity patterns across a broad span of ventral temporal (VT) cortex, including fusiform gyrus and lateral occipital areas (LOC), were required for identity discrimination. For viewpoint, five of six viewpoint pairs were discriminated neurally. Viewpoint discrimination was most accurate with a broad span of VT cortex, but the neural and perceptual discrimination patterns differed. Less accurate discrimination of viewpoint, more consistent with human perception, was found in right posterior superior temporal sulcus, suggesting redundant viewpoint codes optimized for different functions. This study provides the first evidence that it is possible to dissociate neural activation patterns for identity and viewpoint independently.
Interferential Impact of ENSO and PDO on Dry and Wet Conditions in the U.S. Great Plains
Authors: Hu Zeng-Zhen , Huang Bohua 
Published: July 2009

Abstract
The influence of the El Niño–Southern Oscillation (ENSO) and Pacific decadal oscillation (PDO) interference on the dry and wet conditions in the Great Plains of the United States has been examined using monthly observational datasets. It is shown that both ENSO and PDO can generate a similar pattern of atmospheric and oceanic anomalies over the eastern part of the North Pacific and western North America that has significant impact on the climate over the Great Plains. Furthermore, the relationship between ENSO–PDO and climate anomalies in the Great Plains is intensified when ENSO and PDO are in phase (El Niño and warm PDO or La Niña and cold PDO). On average, anomalies over the Great Plains favor wet (dry) conditions when both ENSO and PDO are in the positive (negative) phase. However, when ENSO and PDO are out of phase, the relationship is weakened and the anomalies over the Great Plains tend toward neutral. Without ENSO, PDO alone does not affect the North American climate significantly. The relationship is quite robust for different seasons, with the strongest effects for the months of spring and the weakest effects for the months of autumn, whereas the months of winter and summer fall in between. The seasonality of the relationship may be associated with the seasonal dependence of the anomalies of general circulation and the pattern of mean seasonal cycle in the North Pacific.
The contrasting impact of the interference of ENSO and PDO on the climate anomalies in the Great Plains is associated with differences in the ocean–atmosphere anomalies. When ENSO and PDO are in phase, the sea surface temperature (SST) anomalies extend from the equatorial Pacific to the higher latitudes of the North Pacific via the eastern ocean. The distribution of the corresponding anomalies of sea level pressure (SLP) and the wind at 1000 hPa form an ellipse with a southeast–northwest orientation of the long axis because the SST anomalies promote coherent changes in SLP in the central North Pacific. In the upper troposphere, a similar teleconnection pattern with the same sign generated by ENSO and PDO is overlapped and enhanced, which favors anomaly (dry and wet) conditions in the Great Plains. However, when ENSO and PDO are out of phase, the SST anomalies have the same sign in the tropical and central North Pacific, which is opposite to the anomalies near the west coast of North America. The anomalously cyclonic circulation over the North Pacific is weaker in the out-of-phase situation than in the in-phase situation. The distribution of the anomalies of SLP and the wind at 1000 hPa resembles a circle. Meanwhile, in the upper troposphere, ENSO and PDO generate a similar teleconnection pattern with opposite sign, causing cancellation of the anomalous circulation and favoring neutral climate in the Great Plains.
Effect of ENSO Phase on Large-Scale Snow Water Equivalent Distribution in a GCM
Authors: Clifford Debbie , Gurney Robert , Haines Keith 
Published: July 2009

Abstract
Understanding links between the El Niño–Southern Oscillation (ENSO) and snow would be useful for seasonal forecasting, as well as for understanding natural variability and interpreting climate change predictions. Here, a 545-yr run of the third climate configuration of the Met Office Unified Model (HadCM3), with prescribed external forcings and fixed greenhouse gas concentrations, is used to explore the impact of ENSO on snow water equivalent (SWE) anomalies. In North America, positive ENSO events reduce the mean SWE and skew the distribution toward lower values, and vice versa during negative ENSO events. This is associated with a dipole SWE anomaly structure, with anomalies of opposite sign centered in western Canada and the central United States. In Eurasia, warm episodes lead to a more positively skewed distribution and the mean SWE is raised. Again, the opposite effect is seen during cold episodes. In Eurasia the largest anomalies are concentrated in the Himalayas. These correlations with February SWE distribution are seen to exist from the previous June–July–August (JJA) ENSO index onward, and are weakly detected in 50-yr subsections of the control run, but only a shifted North American response can be detected in the analysis of the 40-yr ECMWF Re-Analysis (ERA-40). The ENSO signal in SWE from the long run could still contribute to regional predictions, although it would only be a weak indicator.
PERSIANN-MSA: A Precipitation Estimation Method from Satellite-Based Multispectral Analysis
Authors: Behrangi Ali , Hsu Kuo-lin , Imam Bisher , Sorooshian Soroosh , Huffman George J. , Kuligowski Robert J. 
Published: July 2009

Abstract
Visible and infrared data obtained from instruments onboard geostationary satellites have been extensively used for monitoring clouds and their evolution. The Advanced Baseline Imager (ABI) that will be launched onboard the Geostationary Operational Environmental Satellite-R (GOES-R) series in the near future will offer a larger range of spectral bands; hence, it will provide observations of cloud and rain systems at even finer spatial, temporal, and spectral resolutions than are possible with the current GOES. In this paper, a new method called Precipitation Estimation from Remotely Sensed information using Artificial Neural Networks–Multispectral Analysis (PERSIANN-MSA) is proposed to evaluate the effect of using multispectral imagery on precipitation estimation. The proposed approach uses a self-organizing feature map (SOFM) to classify multidimensional input information, extracted from each grid box and corresponding textural features of multispectral bands. In addition, principal component analysis (PCA) is used to reduce the dimensionality to a few independent input features while preserving most of the variations of all input information. The above method is applied to estimate rainfall using multiple channels of the Spinning Enhanced Visible and Infrared Imager (SEVIRI) onboard the Meteosat Second Generation (MSG) satellite. In comparison to the use of a single thermal infrared channel, the analysis shows that using multispectral data has the potential to improve rain detection and estimation skills with an average of more than 50% gain in equitable threat score for rain/no-rain detection, and more than 20% gain in correlation coefficient associated with rain-rate estimation.
Assimilating Altimetry Data into a HYCOM Model of the Pacific: Ensemble Optimal Interpolation versus Ensemble Kalman Filter
Authors: Wan Liying , Bertino Laurent , Zhu Jiang 
Published: July 2009

Abstract
The ensemble Kalman filter (EnKF) has proven its efficiency in strongly nonlinear dynamical systems but is demanding in its computing power requirements, which are typically about the same as those of the four-dimensional variational data assimilation (4DVAR) systems presently used in several weather forecasting centers. A simplified version of EnKF, the so-called ensemble optimal interpolation (EnOI), requires only a small fraction of the computing cost of the EnKF, but makes the crude assumption of no dynamical evolution of the errors. How do both these two methods compare in realistic settings of a Pacific Ocean forecasting system where the computational cost is a primary concern? In this paper the two methods are used to assimilate real altimetry data via a Hybrid Coordinate Ocean Model of the Pacific. The results are validated against the independent Argo temperature and salinity profiles and show that the EnKF has the advantage in terms of both temperature and salinity and in all parts of the domain, although not with a very striking difference.
Cloud-System-Resolving Model Simulations of Tropical Cloud Systems Observed during the Tropical Warm Pool-International Cloud Experiment
Authors: Wapler Kathrin , Lane Todd P. , May Peter T. , Jakob Christian , Manton Michael J. , Siems Steven T. 
Published: July 2009

Abstract
Nested cloud-system-resolving model simulations of tropical convective clouds observed during the recent Tropical Warm Pool-International Cloud Experiment (TWP-ICE) are conducted using the Weather Research and Forecasting (WRF) model. The WRF model is configured with a highest-resolving domain that uses 1.3-km grid spacing and is centered over Darwin, Australia. The performance of the model in simulating two different convective regimes observed during TWP-ICE is considered. The first regime is characteristic of the active monsoon, which features widespread cloud cover that is similar to maritime convection. The second regime is a monsoon break, which contains intense localized systems that are representative of diurnally forced continental convection. Many aspects of the model performance are considered, including their sensitivity to physical parameterizations and initialization time, and the spatial statistics of rainfall accumulations and the rain-rate distribution. While the simulations highlight many challenges and difficulties in correctly modeling the convection in the two regimes, they show that provided the mesoscale environment is adequately reproduced by the model, the statistics of the simulated rainfall agrees reasonably well with the observations.
Energy Transformation and Diabatic Processes in Developing and Nondeveloping African Easterly Waves Observed during the NAMMA Project of 2006
Authors: Ross Robert S. , Krishnamurti T. N. , Pattnaik S. , Simon A. 
Published: July 2009

Abstract
This paper provides an understanding of essential differences between developing and nondeveloping African easterly waves, which was a major goal of NAMMA, NASA’s field program in the eastern Atlantic, which functioned as an extension of the African Monsoon Multidisciplinary Analysis (AMMA) program during 2006.
Three NAMMA waves are studied in detail using FNL analysis: NAMMA wave 2, which developed into Tropical Storm Debby; NAMMA wave 7, which developed into Hurricane Helene; and NAMMA wave 4, which did not develop within the NAMMA domain. Diagnostic calculations are performed on the analyzed fields using energy transformation equations and the isentropic potential vorticity equation.
The results show that the two developing waves possess clear and robust positive barotropic energy conversion in conjunction with positive diabatic heating that includes a singular burst of heating at a particular time in the wave’s history. This positive barotropic energy conversion is facilitated in waves that have a northeast–southwest tilt to the trough axis and a wind maximum to the west of this axis. The nondeveloping wave is found to have the same singular burst of diabatic heating at one point in its history, but development of the wave does not occur due to negative barotropic energy conversion. Such conversion is facilitated by a northwest–southeast tilt to the trough axis and a wind maximum to the east of this axis.
The conclusions about wave development and nondevelopment formulated in this research are viewed as important and significant, but they require additional testing with detailed observational- and numerical-based studies.
A `pad' sensor for measuring the moisture content of building materials
Authors: Davies M. , Ye Z. 
Published: July 2009

A range of techniques are available to measure the moisture content of building fabrics. Indeed a thermal dual-probe has been successfully developed by the authors for measuring the moisture content of building fabrics in situ. However, the dual-probe technique has limitations due to the fact that two, small diameter, parallel holes must be drilled for inserting the dual-probe into the material to be measured. It is difficult to drill such holes accurately in many building envelops and thus, such a dual-probe may be most useful for relatively `soft' materials, such as aerated concrete. This paper describes the `proof of concept' of an innovation that surmounts this problem via the development of a `pad' sensor. Instead of the `traditional' dual-probe approach, the heater and temperature sensor are fixed to the surface of a thermally insulated block. The most important benefit of the pad sensor approach is that no holes are required to be drilled in the material of interest. The moisture content of the building envelops can be measured simply by placing the pad sensor against the wall surface. Extensive finite element modelling was carried out to design the pad sensor and a prototype built. Initial experimental work was then carried out to test the pad sensor — the measured moisture content obtained from the pad sensor was compared with the results of a series of gravimetric analyses. Encouraging agreement between the two methods was obtained. Practical application: A thermal dual-probe has been successfully developed by the authors for measuring the moisture content of building fabrics in situ. However, the dual-probe technique has limitations due to the fact that two, small diameter, parallel holes must be drilled for inserting the dual-probe into the material to be measured. This paper describes an innovation that surmounts this problem via the development of a `pad' sensor. The moisture content of the building envelopes can be accurately measured simply by placing the pad sensor against the wall surface. The pad sensor offers a non-destructive method to measure the moisture content and thus is potentially attractive for applications where this issue is of importance e.g. monitoring the moisture content of historical buildings.