bluuebunny/crossref_metadata_2025_split huggingface dataset:
Within‐laboratory reproducibility of Ames test results: Are repeat tests necessary?
Authors: Zeiger Errol , Mitchell Constance A. , Pfuhler Stefan , Liao Yang , Witt Kristine L. 
Published: April 2024

AbstractThe Ames test is required by regulatory agencies worldwide for assessing the mutagenic and carcinogenic potential of chemical compounds. This test uses several strains of bacteria to evaluate mutation induction: positive results in the assay are predictive of rodent carcinogenicity. As an initial step to understanding how well the assay may detect mutagens present as constituents of complex mixtures such as botanical extracts, a cross‐sector working group examined the within‐laboratory reproducibility of the Ames test using the extensive, publicly available National Toxicology Program (NTP) Ames test database comprising more than 3000 distinct test articles, most of which are individual chemicals. This study focused primarily on NTP tests conducted using the standard Organization for Economic Co‐operation and Development Test Guideline 471 preincubation test protocol with 10% rat liver S9 for metabolic activation, although 30% rat S9 and 10 and 30% hamster liver S9 were also evaluated. The reproducibility of initial negative responses in all strains with and without 10% S9, was quite high, ranging from 95% to 99% with few exceptions. The within‐laboratory reproducibility of initial positive responses for strains TA98 and TA100 with and without 10% rat liver S9 was ≥90%. Similar results were seen with hamster S9. As expected, the reproducibility of initial equivocal responses was lower, <50%. These results will provide context for determining the optimal design of recommended test protocols for use in screening both individual chemicals and complex mixtures, including botanicals.
Accounting for the power of nature: Using flume and field studies to compare the capacities of bio‐energy and fluvial energy to move surficial gravels
Authors: Johnson Matthew F. , Albertson Lindsey K. , Everall Nicholas C. , Harvey Gemma L. , Mason Richard , Pledger Andrew , Rice Stephen P. , Thorne Colin R. 
Published: April 2024

AbstractRiver channels, riparian and floodplain forms and dynamics are all influenced strongly by biological processes. However, the influence of macroinvertebrates on entrainment and transport of river sediments remains poorly understood. We use an energy‐based approach to explore the capacity of benthic animals to move surficial, gravel‐bed particles in field and laboratory settings and use the results to assess the relative significance of biological and physical benthic processes. Our results showed that in 11 British gravel‐bed rivers, the maximum energy content (i.e., calorific content) of macroinvertebrate communities generally matched the flow energy associated with median discharges and, at multiple sites, exceeded that of the 10‐year return interval flood. A series of laboratory experiments used to estimate the minimum energy expended by signal crayfish (Pacifastacus leniusculus) when performing geomorphic work established that crayfish move gravel particles at energy levels below that expected of the flow, complicating direct comparisons of the capacity for macroinvertebrates and fluvial flows to influence bed mobility. Our findings suggest that the influence of macroinvertebrate communities in either promoting or suppressing, the mobilisation of the bed may be large compared to equivalent values of fluvial energy. Based on these findings, we conclude that in the gravel‐bed rivers studied, the macroinvertebrate community's potential to perform geomorphic work matches or exceeds the stream power during most of the year. Although our study examined biological and fluvial energy systems separately, it is important to recognise that in nature, these systems are highly interactive. It follows that utilising the energy framework presented in this paper could lead to rapid advances in both fluvial biogeomorphology and river management and restoration.
Attributes of sensory and instrumental analysis that determine overall liking of fresh orange varieties with different maturity index
Authors: Baviera‐Puig Amparo , López‐Cortés Isabel , Ortolá María Dolores , Buitrago‐Vera Juan 
Published: April 2024

AbstractSpain is the leading exporter of fresh citrus for consumption. In order to adapt to market needs, it is key to analyze the attributes that most influence each variety, the time of ripening and the color of the fruit. In this way, the producer can select the right fruit for each moment of the season. The main objective was to evaluate the fruit quality of three interesting orange tree varieties for fresh consumption: “Washington Navel,” “Navelate,” and “Lanelate” sweet orange. To achieve this goal, sensory analysis was combined with instrumental analysis. In Washington Navel, the maturity index provided a positive contribution to overall liking. Both herbaceous and fruity aroma have a negative influence on the overall liking score. In Navelina, the color and aromas (herbaceous and fruity) played an important positive role in overall liking. The importance of this smell could be related to the freshness of the oranges. In contrast, the maturity index provided a negative contribution. Lanelate is the only variety in which vitamin C provided a positive contribution to overall liking. However, L* value showed a negative contribution. Therefore, there were clearly different characteristics among varieties that were detected. So far, different orange varieties have not been compared according to their maturity index and using sensory analysis. Descriptive analysis and the variables that influence overall liking are fundamental to adapt to consumer expectations. The results obtained would help to select the fruit with better acceptability in the market.
Future perspectives of radiation therapy for Hodgkin Lymphoma: Risk‐adapted, response‐adapted, and safer than before
Authors: Saddi Jessica , Barcellini Amelia , Gotti Manuel , Mazzacane Alessandro , Tolva Alessandra , Lazic Tanja , Arcaini Luca , Zecca Marco , Orlandi Ester , Filippi Andrea Riccardo 
Published: April 2024

AbstractClassical Hodgkin lymphoma is a lymphoproliferative disease with a good prognosis mainly seen in young people. Nevertheless secondary malignancy, cardiac disease and infertility may affect the long survivors with significant impact on quality of life, morbidity and overall survival. In the last decades several treatment strategies were evaluated to reduce the toxicity of first line treatment such as avoiding radiotherapy or its reduction in terms of dosage and extension. Many trials including interim Positron Emission Tomography evaluation fail to compare efficacy between combined modality treatment versus chemotherapy alone in particular in early stage disease. In this review we analyze which subset of patients could take advantage from proton therapy in terms of toxicity and cost effectiveness.
Effects of physician‐present prehospital care in patients with out‐of‐hospital cardiac arrest on return of spontaneous circulation: A retrospective, observational study in Saga, Japan
Authors: Shinada Kota , Matsuoka Ayaka , Miike Toru , Koami Hiroyuki , Sakamoto Yuichiro 
Published: April 2024

AbstractBackground and AimsEmergency medical services for out‐of‐hospital cardiac arrest (OHCA) vary according to region and country, and patient prognosis differs accordingly. In Japan, physicians may provide prehospital care. However, the effect of physician‐present prehospital care on achieving return of spontaneous circulation (ROSC) in patients with cardiac arrest is not clear. Here, we aimed to examine the effect of physician‐present prehospital care on the prognosis of patients with OHCA at our hospital compared with physician‐absent care.MethodsIn this retrospective, observational study, patients aged ≥18 years with non‐traumatic OHCA from a single center in Saga City, Japan, between April 2011 and December 2019, were included. Patients were divided into two groups, based on prehospital physician presence or absence. Logistic regression analysis was used to determine the association between physician‐present prehospital care and ROSC.ResultsOf 820 patients with OHCA, 151 had a physician present and 669 did not. Logistic regression analysis with no adjustment showed that the odds ratio (OR) of physician‐present prehospital care for an increased ROSC rate was 1.74 (95% confidence interval [CI]: 1.22−2.48, p = 0.002). Logistic‐regression analysis adjusted for ROSC‐related factors indicated an OR of 1.05 (95% CI: 0.47−2.34, p = 0.914) for physician‐present prehospital care to ROSC.ConclusionPhysician‐present prehospital care may not necessarily lead to increased ROSC rates. However, insufficient data limited our study findings. Further studies involving larger sample sizes are warranted.
Availability of pesticide-treated seeds on the soil surface in different crops and countries: A comprehensive data set reflecting modern agronomic practice
Authors: Hahne Jörg , Foudoulakis Manousos , Kragten Steven , Sprenger Dennis , Ristau Kai , Dietrich Christian , Wang Magnus 
Published: April 2024

Abstract
For plant protection products applied as seed treatments, the risk to birds and mammals possibly feeding on such treated seeds needs to be addressed in the EU, in order to register these products for commercial use. For this purpose, the European Food Safety Food Authority (EFSA) has provided guidance on how to execute such a risk assessment. The risk assessment follows a tiered approach. In the Tier 1 risk assessment of the EFSA guidance (2023), it is assumed that birds or mammals have ad libitum access to treated seeds and exclusively feed on treated seeds. Due to this conservative assumption, the Tier I risk assessment typically indicates an unacceptable risk to birds and mammals and higher-tier refinements are required. One option for refinement is to use data on the availability of treated seeds on the soil surface directly after drilling. Published data on seed counts are, however, limited to a few countries and crops, and often these data are not contemporary, that is, do not reflect advances in sowing technology and current agronomic practice. To address this data gap, we provide recently generated data from industry field trials (the studies were conducted from 2000 to 2022, &gt;70% between 2019 and 2022), covering 270 fields from seven countries (Austria, France, Germany, Hungary, Poland, Spain, UK) for spring and winter cereals, winter oilseed rape, and sunflower. This comprehensive data set realistically reflects modern agronomic practice and is thus suitable for consideration in a regulatory context for refining the risk assessment for birds and mammals. Integr Environ Assess Manag 2024;20:1706–1714. © 2024 The Authors. Integrated Environmental Assessment and Management published by Wiley Periodicals LLC on behalf of Society of Environmental Toxicology & Chemistry (SETAC).
Key Points
In the context of the wildlife risk assessment for pesticides, we have collected data from recently conducted industry studies on seed counts after drilling. The new data set is used to obtain up-to-date seed incorporation efficiencies (i.e., seeds placed into the soil versus seeds left on soil surface) and counts of treated seeds on the soil surface after drilling. This data set included seed counts for crops for which previously no data were publicly available.
TP53INP2 knockdown inhibits inflammatory response and apoptosis after spinal cord injury
Authors: Sun Penghao , Chen Jinchuan , Qin Rujie 
Published: April 2024

AbstractBackgroundSpinal cord injury (SCI) is a traumatic neurological disorder with limited therapeutic options. Tumor protein p53‐inducible nuclear protein 2 (TP53INP2) is involved in the occurrence and development of various diseases, and it may play a role during SCI via affecting inflammation and neuronal apoptosis. This study investigated the associated roles and mechanisms of TP53INP2 in SCI.MethodsMouse and lipopolysaccharide (LPS)‐induced SCI BV‐2 cell models were constructed to explore the role of TP53INP2 in SCI and the associated mechanisms. Histopathological evaluation of spinal cord tissue was detected by hematoxylin and eosin staining. The Basso, Beattie, and Bresnahan score was used to measure the motor function of the mice, while the spinal cord water content was used to assess spinal cord edema. The expression of TP53INP2 was measured using RT‐qPCR. In addition, inflammatory factors in the spinal cord tissue of SCI mice and LPS‐treated BV‐2 cells were measured using enzyme‐linked immunosorbent assay. Apoptosis and related protein expression levels were detected by flow cytometry and western blot analysis, respectively.ResultsTP53INP2 levels increased in SCI mice and LPS‐treated BV‐2 cells. The results of in vivo and in vitro experiments showed that TP53INP2 knockdown inhibited the inflammatory response and neuronal apoptosis in mouse spinal cord tissue or LPS‐induced BV‐2 cells.ConclusionsAfter spinal cord injury, TP53INP2 was upregulated, and TP53INP2 knockdown inhibited the inflammatory response and apoptosis.
Screening of single nucleotide polymorphism in matrix metalloproteinase‐2 (MMP2) and tetraspanin CD63 genes in Chlamydia trachomatis‐infected tubal ectopic pregnancy patients
Authors: Pant Shipra , Bhati Tanu , Dimri Astha , Arora Renu , Siraj Fouzia , Rastogi Sangita 
Published: April 2024

AbstractObjectiveTubal ectopic pregnancy (EP) is a leading cause of maternal morbidity and mortality. Studies have suggested that infection‐induced inflammatory responses are major risk factors for EP. The aim of the present study was to find an association between MMP2 and CD63 gene variants and risk of EP during Chlamydia trachomatis infection in an Indian population.MethodsFallopian tube samples of 120 EP and 120 tubal ligation women were collected. C. trachomatis was detected by PCR. The genotyping of MMP2 (rs17859882 G/T, rs7201A/C) and CD63(rs2231464 C/T, rs376086542 A/G) gene variants was done by qualitative real‐time PCR using allelic discrimination method (VIC‐ and FAM‐labeled).ResultsThe frequency of GG or GT genotype of MMP2 G/T polymorphism (rs17859882) was 66.6% in infected EP and 36.7% in uninfected EP and 22% in tubal ligation controls (P < 0.0001), while the frequency of AC or CC genotype of MMP2 A/C polymorphism (rs7201) was 66.6% in infected EP and 20.6% in uninfected EP and 13.5% in tubal ligation controls (P < 0.0001). The frequency of CT or TT genotype of CD63 C/T polymorphism (rs2231464) was 74% in infected EP and 21.8% in uninfected EP and 11.8% tubal ligation controls (P < 0.0001), while the frequency of AG or GG genotype of CD63 A/G polymorphism (rs376086542) was 48.1% in infected EP and 41.3% in uninfected EP and 18.6% tubal ligation controls (P < 0.0001).ConclusionsThe present study revealed a strong association between the presence of gene variants MMP2 (rs17859882 G/T, rs7201A/C) and CD63 (rs2231464 C/T, rs376086542 A/G) and risk of tubal EP during C. trachomatis infection.
Conservative management or cesarean hysterectomy for placenta accreta spectrum in middle‐income countries: A systematic review and meta‐analysis
Authors: Yu Huihui , Diao Jingyi , Fei Jiajia , Wang Xingxing , Li Dan , Yin Zongzhi 
Published: April 2024

AbstractBackgroundCesarean hysterectomy is a dominant and effective approach during delivery in patients with placenta accreta spectrum (PAS). However, as hysterectomy results in a loss of fertility, conservative management is an alternative approach. However, management selection may be affected by a country's overall economic level. Thus the preferred treatment for PAS generates controversy in middle‐income countries.ObjectivesWe aimed to compare conservative management and cesarean hysterectomy for managing PAS in middle‐income countries.Search StrategyChina National Knowledge Infrastructure, Wanfang Med Online Databases, Cochrane Library, Ovid MEDLINE, PubMed, Web of Science, EMBASE, clinicaltrials.gov, and Scopus were searched from inception through to October 1, 2022.Selection CriteriaWe included studies that evaluated at least one complication comparing conservative management and hysterectomy. All cases were diagnosed with PAS prenatally and intraoperatively.Data Collection and AnalysisThe primary outcomes were blood loss, adjacent organ damage, and the incidence of hysterectomy. Descriptive analyses were conducted for studies that did not meet the meta‐analysis criteria. A fixed‐effects model was used for studies without heterogeneity and a random‐effects model was used for studies with statistical heterogeneity.Main ResultsIn all, 11 observational studies were included, with 975 and 625 patients who underwent conservative management and cesarean hysterectomy, respectively. Conservative management was significantly associated with decreased blood loss and lower risks of adjacent organ injury and hysterectomy. Conservative management significantly reduced blood transfusions, hospitalization duration, operative time, intensive care unit admission rates, and infections. There were no significant differences in the risks of coagulopathy, thromboembolism, or reoperation.ConclusionGiven short‐term complications and future fertility preferences for patients, conservative management appears to effectively manage PAS in middle‐income countries. Owing to low levels of evidence, high heterogeneity and insufficient long‐term follow‐up data, further detailed studies are warranted.
Parental Reflective Functioning on the Parent Development Interview: A narrative review of measurement, association, and future directions
Authors: Slade Arietta , Sleed Michelle 
Published: April 2024

AbstractA caregiver's capacity to mentalize is thought to be one of the most important features of secure parent–child relationships. Parental mentalizing can be measured using the Reflective Functioning (RF) coding system applied to the Parent Development Interview (PDI). In this narrative review, we summarize the research using this measure and synthesize what has been learnt about the predictors, correlates and sequelae of parental RF. Studies have consistently shown that PRF on the PDI is associated with both parent and child attachment and is an important factor in the intergenerational transmission of attachment. It is also related to the quality of parental representations, parent–child interactions, and child outcomes. While a number of social and clinical risk factors are associated with lower PRF, it is difficult to disentangle the unique contribution of each of these. We discuss these findings and present the direction of future work that is planned to expand and refine the PRF scale for the PDI.
Two‐electron redox chemistry enables potassium‐free copper hexacyanoferrate as high‐capacity cathode for aqueous Mg‐ion battery
Authors: Ling Ying , He Bing , Han Lijie , Gong Wenbin , Chang Chaofeng , Zhang Qichong 
Published: April 2024

AbstractPrussian blue analogs (PBAs) are potential contestants for aqueous Mg‐ion batteries (AMIBs) on account of their high discharge voltage and three‐dimensional open frameworks. However, the low capacity arising from single reaction site severely restricts PBAs' practical applications in high‐energy‐density AMIBs. Here, an organic acid co‐coordination combined with etching method is reported to fabricate defect‐rich potassium‐free copper hexacyanoferrate with structural water on carbon nanotube fiber (D‐CuHCF@CNTF). Benefiting from the high‐valence‐state reactive sites, arrayed structure and defect effect, the well‐designed D‐CuHCF@CNTF exhibits an extraordinary reversible capacity of 146.6 mAh g−1 with two‐electron reaction, nearly close to its theoretical capacity. It is interesting to unlock the reaction mechanism of the Fe2+/Fe3+ and Cu+/Cu2+ redox couples via x‐ray photoelectron spectroscopy. Furthermore, density functional theory calculations reveal that Fe and Cu in potassium‐free D‐CuHCF participate in charge transfer during the Mg2+ insertion/extraction process. As a proof‐of‐concept demonstration, a rocking‐chair fiber‐shaped AMIBs was constructed via coupling with the NaTi2(PO4)3/CNTF anode, achieving high energy density and impressive mechanical flexibility. This work provides new possibilities to develop potassium‐free PBAs with dual‐active sites as high‐capacity cathodes for wearable AMIBs.image
Characterizing the Nonlinear Pharmacokinetics and Pharmacodynamics of BI 187004, an 11β‐Hydroxysteroid Dehydrogenase Type 1 Inhibitor, in Humans by a Target‐Mediated Drug Disposition Model
Authors: Yuan Xuanzhen , An Guohua 
Published: April 2024

AbstractBI 187004, a selective small‐molecule inhibitor of 11β‐hydroxysteroid dehydrogenase‐1 (11β‐HSD1), displayed complex nonlinear pharmacokinetics (PK) in humans. Following nine single oral doses, BI 187004 exhibited nonlinear PK at low doses and linear PK at higher doses. Notably, substantial hepatic 11β‐HSD1 inhibition (50%) was detected in a very low‐dose group, achieving a consistent 70% hepatic enzyme inhibition in subsequent ascending doses without any dose‐dependent effects. The unusual PK and PD profiles of BI 187004 suggest the presence of pharmacological target‐mediated drug disposition (TMDD), arising from the saturable binding of BI 187004 compound to its high‐affinity and low‐capacity target 11β‐HSD1. The non‐intuitive dose, exposure, and response relationship for BI 187004 pose a significant challenge in rational dose selection. This study aimed to construct a TMDD model to explain the complex nonlinear PK behavior and underscore the importance of recognizing TMDD in this small‐molecule compound. Among the various models explored, the best model was a two‐compartment TMDD model with three transit absorption components. The final model provides insights into 11β‐HSD1 binding‐related parameters for BI 187004, including the total amount of 11β‐HSD1 in the liver (estimated to be 8000 nmol), the second order association rate constant (estimated to be 0.102 nM−1h−1), and the first‐order dissociation rate constant (estimated to be 0.11 h−1). Our final population PK model successfully characterized the intricate nonlinear PK of BI 187004 across a wide dose range. This modeling work serves as a valuable reference for the rational selection of the dose regimens for BI 187004's future clinical trials.