bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bioactivity-guided identification and cell signaling technology to delineate the immunomodulatory effects of Panax ginseng on human promonocytic U937 cells
Authors: Lee Davy CW , Yang Cindy LH , Chik Stanley CC , Li James CB , Rong Jian-hui , Chan Godfrey CF , Lau Allan SY 
Published: May 2009

AbstractBackgroundGinseng is believed to have beneficial effects against human diseases, and its active components, ginsenosides, may play critical roles in its diverse physiological actions. However, the mechanisms underlying ginseng's effects remain to be investigated. We hypothesize some biological effects of ginseng are due to its anti-inflammatory effects.MethodsHuman promonocytic U937 cells were used to investigate the immunomodulatory effects of ginseng following TNF-α treatment. A global gene expression profile was obtained by using genechip analysis, and specific cytokine expression was measured by quantitative RT-PCR and ELISA. HPLC was used to define the composition of ginsenosides in 70% ethanol-water extracts of ginseng. Activation of signalling kinases was examined by Western blot analysis.ResultsSeventy percent ethanol-water extracts of ginseng significantly inhibited the transcription and secretion of CXCL-10 following TNF-α stimulation. Nine ginsenosides including Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, Rg3and Rh1were identified in our extract by HPLC. Seven out of nine ginsenosides could significantly inhibit TNF-α-induced CXCL-10 expression in U937 cells and give comparable inhibition of CXCL-10 transcription to those with the extract. However, the CXCL-10 suppressive effect of individual ginsenosides was less than that of the crude extract or the mixture of ginsenosides. The CXCL-10 suppression can be correlated with the inactivation of ERK1/2 pathways by ginseng.ConclusionWe showed ginseng suppressed part of the TNF-α-inducible cytokines and signalling proteins in promonocytic cells, suggesting that it exerts its anti-inflammatory property targeting at different levels of TNF-α activity. The anti-inflammatory role of ginseng may be due to the combined effects of ginsenosides, contributing in part to the diverse actions of ginseng in humans.
Detection of hepatitis E virus in wild boars of rural and urban regions in Germany and whole genome characterization of an endemic strain
Authors: Schielke Anika , Sachs Katja , Lierz Michael , Appel Bernd , Jansen Andreas , Johne Reimar 
Published: May 2009

Abstract

Background
Hepatitis E is an increasingly diagnosed human disease in Central Europe. Besides domestic pigs, in which hepatitis E virus (HEV) infection is highly prevalent, wild boars have been identified as a possible source of human infection. In order to assess the distribution of HEV in the wild boar population of Germany, we tested liver samples originating from different geographical regions for the presence of the HEV genome and compared the detected sequences to animal and human HEV strains.

Results
A total of 148 wild boar liver samples were tested using real-time RT-PCR resulting in an average HEV detection rate of 14.9% (95% CI 9.6–21.6). HEV was detected in all age classes and all geographical regions. However, the prevalence of HEV infection was significantly higher in rural as compared to urban regions (p < 0.001). Sequencing of the PCR products indicated a high degree of heterogenicity of the detected viruses within genotype 3 and a grouping according to their geographical origin. The whole genome sequence of an HEV isolate (wbGER27) detected in many wild boars in the federal state of Brandenburg was determined. It belongs to genotype 3i and shows 97.9% nucleotide sequence identity to a partial sequence derived from a human hepatitis E patient from Germany.

Conclusion
The results indicate that wild boars have to be considered as a reservoir for HEV in Germany and that a risk of HEV transmission to humans is present in rural as well as urban regions.
Validation of the International Classification of Functioning, Disability and Health Core Set for chronic widespread pain from the perspective of fibromyalgia patients
Authors: Hieblinger Robin , Coenen Michaela , Stucki Gerold , Winkelmann Andreas , Cieza Alarcos 
Published: May 2009

Abstract
Introduction
Functioning is recognized as an important study outcome in chronic widespread pain (CWP). The Comprehensive ICF Core Set for CWP is an application of the International Classification of Functioning, Disability and Health (ICF) with the purpose of representing the typical spectrum of functioning of patients with CWP. The objective of the study was to add evidence to the validation of the Comprehensive ICF Core Set for CWP from the patient perspective. The specific aims were to explore the aspects of functioning and health important to patients with fibromyalgia, and to examine to what extent these aspects are represented by the current version of the Comprehensive ICF Core Set for CWP.

Methods
The sampling of patients followed the maximum variation strategy. Sample size was determined by saturation. The focus groups were digitally recorded and transcribed verbatim. The meaning condensation procedure was used for qualitative data analysis. After qualitative data analysis, the identified concepts were linked to ICF categories.

Results
Thirty-three patients participated in six focus groups. Fifty-four ICF categories out of 67 categories of the Comprehensive ICF Core Set for CWP were reported by the patients. Forty-eight additional categories that are not covered in the Comprehensive ICF Core Set for CWP were raised.

Conclusions
Most ICF categories of the existing version of the Comprehensive ICF Core Set for CWP could be confirmed from the patient perspective. However, several categories not included in the Core Set emerged and should be considered for inclusion.
Gene expression and activity of cartilage degrading glycosidases in human rheumatoid arthritis and osteoarthritis synovial fibroblasts
Authors: Pásztói Mária , Nagy György , Géher Pál , Lakatos Tamás , Tóth Kálmán , Wellinger Károly , Pócza Péter , György Bence , Holub Marianna C , Kittel Ágnes , Pálóczy Krisztina , Mazán Mercédesz , Nyirkos Péter , Falus András , Buzas Edit I 
Published: May 2009

Abstract
Introduction
Similar to matrix metalloproteinases, glycosidases also play a major role in cartilage degradation. Carbohydrate cleavage products, generated by these latter enzymes, are released from degrading cartilage during arthritis. Some of the cleavage products (such as hyaluronate oligosaccharides) have been shown to bind to Toll-like receptors and provide endogenous danger signals, while others (like N-acetyl glucosamine) are reported to have chondroprotective functions. In the current study for the first time we systematically investigated the expression of glycosidases within the joints.

Methods
Expressions of β-D-hexosaminidase, β-D-glucuronidase, hyaluronidase, sperm adhesion molecule 1 and klotho genes were measured in synovial fibroblasts and synovial membrane samples of patients with rheumatoid arthritis and osteoarthritis by real-time PCR. β-D-Glucuronidase, β-D-glucosaminidase and β-D-galactosaminidase activities were characterized using chromogenic or fluorogenic substrates. Synovial fibroblast-derived microvesicles were also tested for glycosidase activity.

Results
According to our data, β-D-hexosaminidase, β-D-glucuronidase, hyaluronidase, and klotho are expressed in the synovial membrane. Hexosaminidase is the major glycosidase expressed within the joints, and it is primarily produced by synovial fibroblasts. HexA subunit gene, one of the two genes encoding for the alpha or the beta chains of hexosaminidase, was characterized by the strongest gene expression. It was followed by the expression of HexB subunit gene and the β-D-glucuronidase gene, while the expression of hyaluronidase-1 gene and the klotho gene was rather low in both synovial fibroblasts and synovial membrane samples. Tumor growth factor-β1 profoundly downregulated glycosidase expression in both rheumatoid arthritis and osteoarthritis derived synovial fibroblasts. In addition, expression of cartilage-degrading glycosidases was moderately downregulated by proinflammatory cytokines including TNFα, IL-1β and IL-17.

Conclusions
According to our present data, glycosidases expressed by synovial membranes and synovial fibroblasts are under negative regulation by some locally expressed cytokines both in rheumatoid arthritis and osteoarthritis. This does not exclude the possibility that these enzymes may contribute significantly to cartilage degradation in both joint diseases if acting in collaboration with the differentially upregulated proteases to deplete cartilage in glycosaminoglycans.
Cytomegalovirus infection in critically ill patients: a systematic review
Authors: Osawa Ryosuke , Singh Nina 
Published: May 2009

Abstract
Introduction
The precise role of cytomegalovirus (CMV) infection in contributing to outcomes in critically ill immunocompetent patients has not been fully defined.

Methods
Studies in which critically ill immunocompetent adults were monitored for CMV infection in the intensive care unit (ICU) were reviewed.

Results
CMV infection occurs in 0 to 36% of critically ill patients, mostly between 4 and 12 days after ICU admission. Potential risk factors for CMV infection include sepsis, requirement of mechanical ventilation, and transfusions. Prolonged mechanical ventilation (21 to 39 days vs. 13 to 24 days) and duration of ICU stay (33 to 69 days vs. 22 to 48 days) correlated significantly with a higher risk of CMV infection. Mortality rates in patients with CMV infection were higher in some but not all studies. Whether CMV produces febrile syndrome or end-organ disease directly in these patients is not known.

Conclusions
CMV infection frequently occurs in critically ill immunocompetent patients and may be associated with poor outcomes. Further studies are warranted to identify subsets of patients who are likely to develop CMV infection and to determine the impact of antiviral agents on clinically meaningful outcomes in these patients.
Three Novel CFTR Polymorphic Repeats Improve Segregation Analysis for Cystic Fibrosis
Authors: Elce Ausilia , Boccia Angelo , Cardillo Giuseppe , Giordano Sonia , Tomaiuolo Rossella , Paolella Giovanni , Castaldo Giuseppe 
Published: May 2009

Abstract
Background: Molecular diagnosis for cystic fibrosis (CF) is based on the direct identification of mutations in the CFTR gene [cystic fibrosis transmembrane conductance regulator (ATP-binding cassette sub-family C, member 7)] (detection rate about 90% with scanning procedures) and on segregation analysis of intragenic polymorphisms for carrier and prenatal diagnosis in about 20% of CF families in which 1 or both causal mutations are unknown.
Methods: We identified 3 novel intragenic polymorphic repeats (IVS3polyA, IVS4polyA, and IVS10CA repeats) in the CFTR gene and developed and validated a procedure based on the PCR followed by capillary electrophoresis for large-scale analysis of these polymorphisms and the 4 previously identified microsatellites (IVS1CA, IVS8CA, IVS17bTA, and IVS17bCA repeats) in a single run. We validated the procedure for both single- and 2-cell samples (for a possible use in preimplantation diagnosis), and on a large number of CF patients bearing different genotypes and non-CF controls.
Results: The allelic distribution and heterozygosity results suggest that the 3 novel polymorphisms strongly contribute to carrier and prenatal diagnosis of CF in families in which 1 or both causal mutations have not been identified. At least 1 of the 4 previously identified microsatellites was informative in 78 of 100 unrelated CF families; at least 1 of all 7 polymorphisms was informative in 98 of the families. Finally, the analysis of haplotypes for the 7 polymorphisms revealed that most CF mutations are associated with different haplotypes, suggesting multiple slippage events but a single origin for most CFTR mutations.
Conclusions: The analysis of the 7 polymorphisms is a rapid and efficient tool for routine carrier, prenatal, and preimplantation diagnosis of CF.
Revisiting journalism as a profession in the 19th century: Empirical findings on women journalists in Central Europe
Authors: Kinnebrock Susanne 
Published: May 2009

Abstract
This contribution raises the question whether journalism at its beginnings was indeed a profession only for men, as much of the research literature suggests. However, the assumption of a “gendered profession” may also be due to gendered research patterns that produce and reproduce a gendered academic discourse on journalism. The study presented here puts these questions to test and investigates the cultural, social and work-related position of female writers in German-speaking countries at the end of the 19th century. The data is based on a complete census collected between 1896 and 1898. In a second step, the occupation and opus of female writers who worked for periodicals will be analyzed along established concepts of journalism in order to illustrate how women are systematically excluded by dominant concepts of what journalism is and journalists actually do.
News for adolescents: Mission impossible? An evaluation of Flemish television news aimed at teenagers
Authors: Vandebosch Heidi , Dhoest Alexander , Van den Bulck Hilde 
Published: May 2009

Abstract
Media companies as well as governments launch initiatives to reverse the decline in news consumption by adolescents. Since 2007, the Flemish government has been funding newscasts for adolescents on two commercial channels, Zoom on VTM and Jam on VT4. In 2008, these programs were evaluated using in-depth interviews with producers, content analysis of 30 episodes of each program, an analysis of the ratings for the first season, and an online survey among 663 adolescents aged 10 to 18. Results indicate that there is a lot of variation within this group, making it hard to please them with a single program. Zoom addresses slightly older adolescents (12–18) with a program closely following the “adult” news, while Jam provides younger adolescents (10–16) with more digestible and entertaining news. Most respondents in the survey liked both programs, among other things because of the strong presence of adolescents on screen. Yet adolescent viewing figures are extremely low, mainly due to the inappropriate place in the schedule.
D3 GH receptor polymorphism is not associated with IGF1 levels in untreated acromegaly
Authors: Kamenicky Peter , Dos Santos Christine , Espinosa Consuelo , Salenave Sylvie , Galland Françoise , Le Bouc Yves , Maison Patrick , Bougnères Pierre , Chanson Philippe 
Published: May 2009

ContextA discrepancy between serum GH and IGF1 concentrations is frequent in patients with acromegaly. Here, we examined whether the exon 3-deleted (d3) GH receptor (GHR) variant, which has been linked to increased responsiveness to GH treatment in short children, influences the GH/IGF1 relationship in patients with acromegaly.ObjectiveTo study the possible influence of the GHR genotype on the GH/IGF1 relationship in untreated acromegalic patients.DesignGHR genotype analysis with retrospective clinical and biochemical data collection performed in a single third-reference medical center.Patients and methodsClinical data were obtained from the medical records of 105 acromegalic patients who had GH and IGF1 assays in the same laboratory and who were genotyped for the full-length (fl) or d3-GHR alleles.ResultsThe distribution of GHR genotypes was 51% fl/fl, 30% fl/d3, and 19% d3/d3. Patients with d3/d3 genotype were younger than the patients in the other two groups (P<0.05). Baseline GH and IGF1 concentrations did not differ among the three groups. The linear correlation between GH and IGF1 concentrations was similar in the three genotypic groups.ConclusionsThe exon 3 GHR genotype does not affect the GH/IGF1 relationship in untreated acromegalic patients with high circulating GH and IGF1 levels.
The spectrum of phenotypes associated with mutations in steroidogenic factor 1 (SF-1, NR5A1, Ad4BP) includes severe penoscrotal hypospadias in 46,XY males without adrenal insufficiency
Authors: Köhler Birgit , Lin Lin , Mazen Inas , Cetindag Cigdem , Biebermann Heike , Akkurt Ilker , Rossi Rainer , Hiort Olaf , Grüters Annette , Achermann John C 
Published: May 2009

ObjectiveHypospadias is a frequent congenital anomaly but in most cases an underlying cause is not found. Steroidogenic factor 1 (SF-1, NR5A1, Ad4BP) is a key regulator of human sex development and an increasing number of SF-1 (NR5A1) mutations are reported in 46,XY disorders of sex development (DSD). We hypothesized that NR5A1 mutations could be identified in boys with hypospadias.Design and methodsMutational analysis of NR5A1 in 60 individuals with varying degrees of hypospadias from the German DSD network.ResultsHeterozygous NR5A1 mutations were found in three out of 60 cases. These three individuals represented the most severe end of the spectrum studied as they presented with penoscrotal hypospadias, variable androgenization of the phallus and undescended testes (three out of 20 cases (15%) with this phenotype). Testosterone was low in all three patients and inhibin B/anti-Müllerian hormone (AMH) were low in two patients. Two patients had a clear male gender assignment. Gender re-assignment to male occurred in the third case. Two patients harbored heterozygous nonsense mutations (p.Q107X/WT, p.E11X/WT). One patient had a heterozygous splice site mutation in intron 2 (c.103-3A/WT) predicted to disrupt the main DNA-binding motif. Functional studies of the nonsense mutants showed impaired transcriptional activation of an SF-1-responsive promoter (Cyp11a). To date, adrenal insufficiency has not occurred in any of the patients.ConclusionsSF-1 (NR5A1) mutations should be considered in 46,XY individuals with severe (penoscrotal) hypospadias, especially if undescended testes, low testosterone, or low inhibin B/AMH levels are present. SF-1 mutations in milder forms of idiopathic hypospadias are unlikely to be common.
Observations Concerning Reproductive Temperature Requirements of Captive Lahontan Cutthroat Trout
Authors: Bigelow John P , Rauw Wendy M , Gomez-Raya Luis 
Published: May 2009

Abstract
The effects of holding temperature during late oocyte development and spawning on the timing of ovulation, fecundity, and egg survival in female Lahontan cutthroat trout Oncorhynchus clarkii henshawi in three postovulatory regimes were investigated. The timing of ovulation was similar for females held at 7.2°C and 12.4°C from 2.5 to 6.0 months before the time of ovulation until spawning. Mean fecundity was higher for females held at 7.2°C. Mean egg eye-up, hatch, and swim-up rates were also higher for females held at 7.2°C. Eggs collected 1, 4, and 7 d postovulation exhibited similar survival rates within females regardless of holding temperature.
Analysis of a Stock–Recruit Relationship for Red King Crab off Kodiak Island, Alaska
Authors: Bechtol William R. , Kruse Gordon H. 
Published: May 2009

Abstract
Waters of the northern Gulf of Alaska around Kodiak Island once supported the world's largest fishery for the red king crab Paralithodes camtschaticus. Commercial fisheries began at low levels in the 1930s, increasing rapidly in the 1960s to a peak harvest of 42,800 metric tons in 1965. Stock abundance declined sharply in the late 1960s, moderated in the 1970s, and crashed in the early 1980s. A commercial fishery closure since 1983 has not resulted in stock recovery. To improve understanding of stock dynamics, we examined spawner–recruit (S-R) relationships for the Kodiak red king crab stock. The shape of the S-R relationship helps describe average stock productivity at different stock levels, thus relating directly to fishery management objectives. Due to limited female data, we used only male data and two currencies of male abundance as a proxy for spawners: either (1) all males greater than or equal to 125 mm carapace length (CL) or (2) legal males (≥145 mm CL). Due to age uncertainty, we considered lag times of 5 to 8 years between reproduction and recruitment. Residuals from fitting a standard Ricker model were strongly negative for brood years from the mid-1970s to the mid-1980s and positive from the early to mid-1990s. A Ricker model with autocorrelated errors resulted in better fits with corrected Akaike's information criterion (AICc) values minimized for a 5-year lag using all males. Among model configurations, estimates of peak production ranged from 2.0 to 3.4 million recruits from a range of 11.3 to 37.7 million males. A model separating the time series into three productivity periods corresponding to different ecological regimes further improved model fit. Although abundances of both spawners and recruits have been less than 1.0 million males since 1985, depensation at low stock sizes was not detected. Future analyses will examine the contribution of ecological and environmental factors to crab recruitment.
Maturation and Reproduction of Shovelnose Sturgeon in the Middle Mississippi River
Authors: Tripp Sara J. , Phelps Quinton E. , Colombo Robert E. , Garvey James E. , Burr Brooks M. , Herzog David P. , Hrabik Robert A. 
Published: May 2009

Abstract
Shovelnose sturgeon Scaphirhynchus platorynchus in the middle Mississippi River provide one of the last commercially viable sturgeon fisheries in the world, yet their maturation and reproduction have not been linked. During 2005 and 2006, we sampled adult and age-0 shovelnose sturgeon to link age at maturation, the timing and periodicity of spawning, age-0 sturgeon production, and the resulting age-0 growth rates. Age at maturity was later than previous estimates, the minimum age of first maturation being 8 years for males and 9 years for females. Total egg count was slightly lower than previously reported (mean = 29,573 per female; SE = 2,472). Males and females typically spawned every 2 and 3 years, respectively. Peaks in mature fish coincided with rising river stages and water temperatures at which shovelnose sturgeon probably spawn. Peaks in spent adults followed. Age-0 shovelnose sturgeon occurred during June and July 2005 and May and June 2006, confirming successful spawning. Age-0 sturgeon grew between 0.69 and 1.69 mm total length/d; four distinct weekly cohorts occurred each year. During fall 2006, females contained ripe eggs, males were milting, and a single age-0 sturgeon (total length = 55 mm) was captured, suggesting that shovelnose sturgeon spawn during fall as well as spring. Management must consider the protracted nature of spawning within seasons as well as differences in spawning activity between seasons.