bluuebunny/crossref_metadata_2025_split huggingface dataset:
Magnetic fields in the early Universe
Authors: Subramanian K. 
Published: December 2009

AbstractWe give a pedagogical introduction to two aspects of magnetic fields in the early Universe. We first focus on how to formulate electrodynamics in curved space time, defining appropriate magnetic and electric fields and writing Maxwell equations in terms of these fields. We then specialize to the case of magnetohydrodynamics in the expanding Universe. We emphasize the usefulness of tetrads in this context. We then review the generation of magnetic fields during the inflationary era, deriving in detail the predicted magnetic and electric spectra for some models. We discuss potential problems arising from back reaction effects and from the large variation of the coupling constants required for such field generation (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Mean electromotive force proportional to mean flow in MILD turbulence
Authors: Rädler K.‐H. , Brandenburg A. 
Published: December 2009

AbstractIn mean‐field magnetohydrodynamics the mean electromotive force due to velocity and magnetic‐field fluctuations plays a crucial role. In general it consists of two parts, one independent of and another one proportional to the mean magnetic field. The first part may be nonzero only in the presence of mhd turbulence, maintained, e.g., by small‐scale dynamo action. It corresponds to a battery, which lets a mean magnetic field grow from zero to a finite value. The second part, which covers, e.g., theαeffect, is important for large‐scale dynamos. Only a few examples of the aforementioned first part of the mean electromotive force have been discussed so far. It is shown that a mean electromotive force proportional to the mean fluid velocity, but independent of the mean magnetic field, may occur in an originally homogeneous isotropic mhd turbulence if there are nonzero correlations of velocity and electric current fluctuations or, what is equivalent, of vorticity and magnetic field fluctuations. This goes beyond the Yoshizawa effect, which consists in the occurrence of mean electromotive forces proportional to the mean vorticity or to the angular velocity defining the Coriolis force in a rotating frame and depends on the cross‐helicity defined by the velocity and magnetic field fluctuations. Contributions to the mean electromotive force due to inhomogeneity of the turbulence are also considered. Possible consequences of the above findings for the generation of magnetic fields in cosmic bodies are discussed (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Swine‐origin pandemic H1N1 influenza virus‐like particles produced in insect cells induce hemagglutination inhibiting antibodies in BALB/c mice
Authors: Krammer Florian , Nakowitsch Sabine , Messner Paul , Palmberger Dieter , Ferko Boris , Grabherr Reingard 
Published: December 2009

AbstractRecent outbreaks of influenza A highlight the importance of rapid and sufficient supply for pandemic and inter‐pandemic vaccines. Classical manufacturing methods for influenza vaccines fail to satisfy this demand. Alternatively, cell culture‐based production systems and virus‐like particle (VLP)‐based technologies have been established. We developed swine‐origin pandemic H1N1 influenza VLPs consisting of hemagglutinin (A/California/04/2009) and matrix protein. Hemagglutinin and matrix protein were co‐expressed in insect cells by the baculovirus expression system. VLPs were harvested from infection supernatants, purified and used for intraperitoneal immunization of BALB/c mice. Immunization induced high serum antibody titers against A/California/04/2009 as well as hemagglutination inhibiting antibodies. Additionally, we compared VLP production in two different insect cell lines, Sf9 and BTI‐TN5B1‐4 (High Five™). Taken together VLPs represent a potential strategy for the fight against new pandemic influenza viruses.
Effects of culture conditions on N‐glycolylneuraminic acid (Neu5Gc) content of a recombinant fusion protein produced in CHO cells
Authors: Borys Michael C. , Dalal Nimish G. , Abu‐Absi Nicholas R. , Khattak Sarwat F. , Jing Ying , Xing Zizhuo , Li Zheng Jian 
Published: December 2009

AbstractCHO cells express glycoproteins containing both the N‐acetylneuraminic acid (Neu5Ac) and minor amounts of the N‐glycolylneuraminic acid (Neu5Gc) forms of sialic acid. As Neu5Gc is not expressed in humans and can be recognized as a foreign epitope, there is the potential for immunogenicity issues for glycoprotein therapeutics. During process development of a glycosylated fusion protein expressed by CHO cells, a number of culture conditions were identified that affected the Neu5Gc content of the recombinant glycoprotein. Sodium butyrate (SB), a well‐known additive reported to enhance recombinant protein productivity in specific cases, minimally affected product titers here, but did decrease Neu5Gc levels by 50–62%. A shift in culture temperature to a lower value after the exponential growth phase was used to extend the culture period. It was found that the Neu5Gc levels were 59% lower when the temperature shift occurred later near the stationary phase of the culture compared to an early‐temperature shift, near the end of the exponential growth phase. Studies on the effects of pCO2 with this product showed that the Neu5Gc levels were 46% lower at high pCO2 conditions (140 mmHg) compared to moderate pCO2 levels (20–80 mmHg). Finally, a comparison of sodium carbonate versus sodium hydroxide as the base used for pH control resulted in a reproducible 33% decrease in Neu5Gc in bioreactors using sodium hydroxide. These results are of practical importance as SB is a commonly tested additive, and the other factors affecting Neu5Gc can conveniently be used to reduce or control Neu5Gc in processes for the manufacture of glycoprotein therapeutics. Biotechnol. Bioeng. 2010;105: 1048–1057. © 2009 Wiley Periodicals, Inc.
Development of a multiresidue method for the determination of multiclass pesticides in soil using GC
Authors: Park Jong‐Hyouk , Mamun Md. Iqbal Rouf , Choi Jeong‐Heui , Abd El‐Aty A. M. , Assayed M. E. , Choi Woo Jung , Yoon Kwang Sik , Han Seong‐Soo , Kim Hee Kweon , Park Byung Jun , Kim Kap Soon , Kim Sang Don , Choi Hun Geun , Shim Jae‐Han 
Published: December 2009

AbstractThe principal objective of the present study was to develop a multiresidue analytical method for 62 pesticides in a soil matrix. Soil samples were fortified with known quantities of pesticides at two different concentration levels (0.1 and 0.01 μg/g) and the analytes were extracted via a liquid–solid extraction method. The pesticides were separated on an HP5 capillary column and were detected by gas chromatography coupled to an electron capture detector (GC‐ECD). The method was validated, considering its good linearities (r2 = 0.978–0.999), specificity and recovery characteristics. Recoveries were found between 70.3 and 113.4% for all pesticides except edifenphos (67.5%) and dichlobenil (69.5%) spiked at a 0.1 μg/mL concentration level and 74.5–117% except ethalfluralin (63.3%) and dichlobenil (51.9%) spiked at a concentration of 0.01 μg/mL. The developed method could be utilized as a simple and cost‐effective method for the routine analysis of 62 pesticides in soil samples. Copyright © 2009 John Wiley & Sons, Ltd.
A multiparallel bioreactor for the cultivation of mammalian cells in a 3D‐ceramic matrix
Authors: Goralczyk Vicky , Driemel Gregor , Bischof Andreas , Peter Andrea , Berthold Almuth , Kroh Lothar , Blessing Lucienne , Schubert Helmut , King Rudibert 
Published: December 2009

AbstractFor adherently growing cells, cultivation is limited by the provided growth surface. Excellent surface‐to‐volume ratios are found in highly porous matrices, which have to face the challenge of nutrient supply inside the matrices' caverns. Therefore, perfusion strategies are recommended which often have to deal with the need of developing an encompassing bioreactor periphery. We present a modular bioreactor system based on a porous ceramic matrix that enables the supply of cells with oxygen and nutrients by perfusion. The present version of the reactor system focuses on simple testing of various inoculation and operation modes. Moreover, it can be used to efficiently test different foam structures. Protocols are given to set‐up the system together with handling procedures for long‐time cultivation of a CHO cell line. Experimental results confirm vital growth of cells inside the matrices' caverns. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Techno‐economic evaluation of a two‐step biological process for hydrogen production
Authors: Ljunggren Mattias , Zacchi Guido 
Published: December 2009

AbstractAn integrated biological process for the production of hydrogen based on thermophilic and photo‐heterotrophic fermentation was evaluated from a technical and economic standpoint. Besides the two fermentation steps the process also includes pretreatment of the raw material (potato steam peels) and purification of hydrogen using amine absorption. The study aimed neither at determining the absolute cost of biohydrogen nor at an economic optimization of the production process, but rather at studying the effects of different parameters on the production costs of biohydrogen as a guideline for future improvements. The effect of the key parameters, hydrogen productivity and yield and substrate concentration in the two fermentations on the cost of the hydrogen produced was studied. The selection of the process conditions was based mainly on laboratory data. The process was simulated by use of the software Aspen Plus and the capital costs were estimated using the program Aspen Icarus Process Evaluator. The study shows that the photo‐fermentation is the main contributor to the hydrogen production cost mainly because of the cost of plastic tubing, for the photo‐fermentors, which represents 40.5% of the hydrogen production cost. The costs of the capital investment and chemicals were also notable contributors to the hydrogen production cost. Major economic improvements could be achieved by increasing the productivity of the two fermentation steps on a medium‐term to long‐term scale. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Using the rate of respiration to monitor events in the infection of Escherichia coli cultures by bacteriophage T4
Authors: Sauvageau Dominic , Allain Béatrice , Cooper David G. 
Published: December 2009

AbstractThe growing interest in applications of bacteriophages creates a need for improvements in the production processes. Continuous monitoring of the phage production is an essential aspect of any control strategy and, at present, there is no completely satisfactory option. The approach presented here uses IR‐spectrometry to continuously measure the rate of respiration (CO2 released) of Escherichia coli infected by phage T4 at various multiplicities of infection (MOI). Within the trends in these data, or in other aspects of the rate of respiration, it was possible to reliably and reproducibly identify five features that reflected specific events in the infection process. These included two events in the host cell apparent growth rate and events in the magnitude of the host cell density, in the measurement of OD600 or in the specific rate of respiration. All of these correlations were within 95% confidence showing that they are suitable for the monitoring and control of E. coli populations infected by phage T4. This method is reliable, cheap, and can be operated in‐line and in real time. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Switchgrass leaching requirements for solid‐state fermentation by Acidothermus cellulolyticus
Authors: VanderGheynst Jean S. , Rezaei Farzaneh , Dooley Todd M. , Berry Alison M. 
Published: December 2009

AbstractGrowth of Acidothermus cellulolyticus in solid‐state fermentation and its required growth conditions were investigated in this study. Extraction of switchgrass was required for growth. Under the experimental conditions, extraction ratio had the most significant effect on the growth of A. cellulolyticus. Heat treatment (in the form of autoclaving) of switchgrass did not have a significant effect on the growth rate; however, longer heat treatment times had a negative effect on the total growth. Moisture content adjustment had no effect on the release of inhibitors into extracts. Our results showed that leaching at a minimum 40:1 (gram water: gram dry biomass) removed inhibitory compound(s) from switchgrass. Upon extraction A. cellulolyticus colonized switchgrass in solid fermentation without exogenous addition of carbon and nitrogen sources. It is the first demonstration of growth of A. cellulolyticus in solid fermentation. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010
pH measurement and a rational and practical pH control strategy for high throughput cell culture system
Authors: Zhou Haiying , Purdie Jennifer , Wang Tongtong , Ouyang Anli 
Published: December 2009

AbstractThe number of therapeutic proteins produced by cell culture in the pharmaceutical industry continues to increase. During the early stages of manufacturing process development, hundreds of clones and various cell culture conditions are evaluated to develop a robust process to identify and select cell lines with high productivity. It is highly desirable to establish a high throughput system to accelerate process development and reduce cost. Multiwell plates and shake flasks are widely used in the industry as the scale down model for large‐scale bioreactors. However, one of the limitations of these two systems is the inability to measure and control pH in a high throughput manner. As pH is an important process parameter for cell culture, this could limit the applications of these scale down model vessels. An economical, rapid, and robust pH measurement method was developed at Eli Lilly and Company by employing SNARF‐4F 5‐(‐and 6)‐carboxylic acid. The method demonstrated the ability to measure the pH values of cell culture samples in a high throughput manner. Based upon the chemical equilibrium of CO2, HCO, and the buffer system, i.e., HEPES, we established a mathematical model to regulate pH in multiwell plates and shake flasks. The model calculates the required %CO2 from the incubator and the amount of sodium bicarbonate to be added to adjust pH to a preset value. The model was validated by experimental data, and pH was accurately regulated by this method. The feasibility of studying the pH effect on cell culture in 96‐well plates and shake flasks was also demonstrated in this study. This work shed light on mini‐bioreactor scale down model construction and paved the way for cell culture process development to improve productivity or product quality using high throughput systems. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Disaggregation of high‐molecular weight species during downstream processing to recover functional monomer
Authors: Xu Xuankuo , Didio David M. , Leister Kirk J. , Ghose Sanchayita 
Published: December 2009

AbstractThe use of chaotropic agents to recover functional monomeric material was investigated for the downstream purification of an Fc‐fusion protein containing high levels of high‐molecular weight (HMW) species. In batch studies, chaotropic agents irreversibly disaggregated a majority of the aggregated protein. An integrated processing mode, termed as on‐column disaggregation, was developed in which the protein was captured on Protein A chromatography and then a chaotropic agent was used to simultaneously elute the bound protein and disaggregate the HMW species. On‐column disaggregation process resulted in protein recoveries of >95% and aggregation reduction of ∼50%. Analytical results are presented showing that the recovered monomeric material was comparable to the reference protein in biochemical, biophysical, and pharmacokinetic properties. The kinetic and molecular mechanisms governing protein aggregation and disaggregation will also be elucidated. For the Fc‐fusion protein studied here, incorporation of the disaggregation strategy in both batch and on‐column modes led to an increase of >10% in overall downstream yield. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010
A cell leakproof PLGA‐collagen hybrid scaffold for cartilage tissue engineering
Authors: Kawazoe Naoki , Inoue Chieko , Tateishi Tetsuya , Chen Guoping 
Published: December 2009

AbstractA cell leakproof porous poly(DL‐lactic‐co‐glycolic acid) (PLGA)‐collagen hybrid scaffold was prepared by wrapping the surfaces of a collagen sponge except the top surface for cell seeding with a bi‐layered PLGA mesh. The PLGA‐collagen hybrid scaffold had a structure consisting of a central collagen sponge formed inside a bi‐layered PLGA mesh cup. The hybrid scaffold showed high mechanical strength. The cell seeding efficiency was 90.0% when human mesenchymal stem cells (MSCs) were seeded in the hybrid scaffold. The central collagen sponge provided enough space for cell loading and supported cell adhesion, while the bi‐layered PLGA mesh cup protected against cell leakage and provided high mechanical strength for the collagen sponge to maintain its shape during cell culture. The MSCs in the hybrid scaffolds showed round cell morphology after 4 weeks culture in chondrogenic induction medium. Immunostaining demonstrated that type II collagen and cartilaginous proteoglycan were detected in the extracellular matrices. Gene expression analyses by real‐time PCR showed that the genes encoding type II collagen, aggrecan, and SOX9 were upregulated. These results indicated that the MSCs differentiated and formed cartilage‐like tissue when being cultured in the cell leakproof PLGA‐collagen hybrid scaffold. The cell leakproof PLGA‐collagen hybrid scaffolds should be useful for applications in cartilage tissue engineering. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Product and contaminant measurement in bioprocess development by SELDI‐MS
Authors: Berrill Alex , Ho Sa V. , Bracewell Daniel G. 
Published: December 2009

AbstractBioprocesses for therapeutic protein production typically require significant resources to be invested in their development. Underlying these efforts are analytical methods, which must be fit for the purpose of monitoring product and contaminants in the process. It is highly desirable, especially in early‐phase development when material and established analytical methods are limiting, to be able to determine what happens to the product and impurities at each process step with small sample volumes in a rapid and readily performed manner. This study evaluates the utility of surface‐enhanced laser desorption ionization mass spectroscopy (SELDI‐MS), known for its rapid analysis and minimal sample volumes, as an analytical process development tool. In‐process samples from an E. coli process for apolipoprotein A‐IM (ApoA‐IM) manufacture were used along with traditional analytical methods such as HPLC to check the SELDI‐MS results. ApoA‐IM is a naturally occurring variant of ApoA‐I that appears to confer protection against cardiovascular disease to those that carry the mutated gene. The results show that, unlike many other analytical methods, SELDI‐MS can handle early process samples that contain complex mixtures of biological molecules with limited sample pretreatment and thereby provide meaningful process‐relevant information. At present, this technique seems most suited to early‐phase development particularly when methods for traditional analytical approaches are still being established. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Impact of freezing on pH of buffered solutions and consequences for monoclonal antibody aggregation
Authors: Kolhe Parag , Amend Elizabeth , K. Singh Satish 
Published: December 2009

AbstractFreezing of biologic drug substance at large scale is an important unit operation that enables manufacturing flexibility and increased use‐period for the material. Stability of the biologic in frozen solutions is associated with a number of issues including potentially destabilizing pH changes. The pH changes arise from temperature‐associated change in the pKas, solubility limitations, eutectic crystallization, and cryoconcentration. The pH changes for most of the common protein formulation buffers in the frozen state have not been systematically measured. Sodium phosphate buffer, a well‐studied system, shows the greatest change in pH when going from +25 to −30°C. Among the other buffers, histidine hydrochloride, sodium acetate, histidine acetate, citrate, and succinate, less than 1 pH unit change (increase) was observed over the temperature range from +25 to −30°C, whereas Tris‐hydrochloride had an ∼1.2 pH unit increase. In general, a steady increase in pH was observed for all these buffers once cooled below 0°C. A formulated IgG2 monoclonal antibody in histidine buffer with added trehalose showed the same pH behavior as the buffer itself. This antibody in various formulations was subject to freeze/thaw cycling representing a wide process (phase transition) time range, reflective of practical situations. Measurement of soluble aggregates after repeated freeze–thaw cycles shows that the change in pH was not a factor for aggregate formation in this case, which instead is governed by the presence or absence of noncrystallizing cryoprotective excipients. In the absence of a cryoprotectant, longer phase transition times lead to higher aggregation. © 2009 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Predicting Muskingum flood routing parameters using spreadsheets
Authors: Karahan Halil 
Published: December 2009

AbstractIn this study, two easy to use and high precision solution methods that can be used for educational and engineering purposes are proposed. The first of these methods is the “Trial–Error with Spreadsheet” procedure combining basic properties of spreadsheets and the other method is the “Nonlinear optimization with Solver” procedure that uses the nonlinear optimization code and the “Iterative calculation” which is one of the most effective properties of spreadsheets. Both of the proposed methods are tested on three samples given in literature and the comparisons of the model results, observation results, and the results of other solution techniques in literature are made. © 2009 Wiley Periodicals, Inc. Comput Appl Eng Educ 20: 280–286, 2012
Using virtual platform in embedded system education
Authors: Lim Heuiseok , Yu HeonChang , Suh Taeweon 
Published: December 2009

AbstractThis article presents our cost‐effective curriculum on embedded systems. Education on embedded systems requires coverage of both hardware and software aspects of the systems. Our curriculum uses one monolithic environment, virtual platform, to introduce all the layers of the system components (i.e., from hardware to operating systems to user applications). It is cost‐effective since a hardware system is replaced by a virtual platform. Correspondingly, hardware boards and a lab space are not required. Yet, students are able to make modifications easily on hardware and software components of interest, fully exercising the system. Students responded to the course survey that they are knowledgeable on how embedded systems work after taking the course. Especially, students responded that the virtual platform is effective to use, in place of a hardware platform to learn embedded systems. The course materials are available to the public from a website at Korea University. © 2009 Wiley Periodicals, Inc. Comput Appl Eng Educ 20: 346–355, 2012