bluuebunny/crossref_metadata_2025_split huggingface dataset:
Controlling Helical Chirality in Atrane Structures: Solvent‐Dependent Chirality Sense in Hemicryptophane‐Oxidovanadium(V) Complexes
Authors: Martinez Alexandre , Robert Vincent , Gornitzka Heinz , Dutasta Jean‐Pierre 
Published: November 2009

AbstractThe diastereomeric hemicryptophane oxidovanadium(V) complexes (P)‐(S,S,S)‐3 and (M)‐(S,S,S)‐4 have been synthesized. 1H and 51V NMR spectra in solution are consistent with the formation of Λ and Δ forms of the propeller‐like vanatrane moiety, leading to two diastereomeric conformers for each complex: that is, (P)‐(S,S,S‐Λ)‐3/(P)‐(S,S,S‐Δ)‐3 and (M)‐(S,S,S‐Λ)‐4/(M)‐(S,S,S‐Δ)‐4. The Λ/Δ ratio is rather temperature‐insensitive but strongly dependent on the solvent (the de of (M)‐(S,S,S)‐4 changes from 0 in benzene to 92 % in DMSO). The solvent therefore controls the preferential clockwise or anticlockwise orientation of the propeller‐like atrane unit. The energy barriers for the Λ⇄Δ equilibrium were determined by NMR experiments, and the highest ΔG≠ value (103.7 kJ mol−1) was obtained for (P)‐(S,S,S)‐3, much higher than those reported for other atrane derivatives. This is attributed to the constraints arising from the cage structure. Determination of the activation parameters provides evidence for a concerted, rather than a stepwise, interconversion mechanism with entropies (ΔS≠) of −243 and −272 J mol−1 K−1 for (P)‐(S,S,S)‐3 and (M)‐(S,S,S)‐4, respectively. The molecular structure of the (P)‐(S,S,S‐Λ)‐3 isomer was solved by X‐ray diffraction and shows a distorted structure with one of the linkers located in the CTV cavity. Complementary quantum chemical calculations were carried out to obtain the energy‐minimized structures of (P)‐(S,S,S)‐3 and (M)‐(S,S,S)‐4. Our density functional theory calculations suggest that the (P)‐(S,S,S‐Λ)‐3 is favored, in agreement with experimental data. For the M series, a similar strategy was used to extract molecular structures and relative energies. As in the case of the P diastereomer, the Λ form dominates over the Δ one.
An Interpenetrated Framework Material with Hysteretic CO2 Uptake
Authors: Mulfort Karen L. , Farha Omar K. , Malliakas Christos D. , Kanatzidis Mercouri G. , Hupp Joseph T. 
Published: November 2009

AbstractA new, twofold interpenetrated metal–organic framework (MOF) material has been synthesized that demonstrates dramatic steps in the adsorption and hysteresis in the desorption of CO2. Measurement of the structure by powder X‐ray diffraction (PXRD) and pair distribution function (PDF) analysis indicates that structural changes upon CO2 sorption most likely involve the interpenetrated frameworks moving with respect to each other.
Variable selection in spatial regression via penalized least squares
Authors: Wang Haonan , Zhu Jun 
Published: November 2009

AbstractWe consider variable selection in linear regression of geostatistical data that arise often in environmental and ecological studies. A penalized least squares procedure is studied for simultaneous variable selection and parameter estimation. Various penalty functions are considered including smoothly clipped absolute deviation. Asymptotic properties of penalized least squares estimates, particularly the oracle properties, are established, under suitable regularity conditions imposed on a random field model for the error process. Moreover, computationally feasible algorithms are proposed for estimating regression coefficients and their standard errors. Finite‐sample properties of the proposed methods are investigated in a simulation study and comparison is made among different penalty functions. The methods are illustrated by an ecological dataset of landcover in Wisconsin. The Canadian Journal of Statistics 37: 607–624; 2009 © 2009 Statistical Society of Canada
Synthesis, Enzymatic Evaluation, and Docking Studies of Fluorogenic Caspase 8 Tetrapeptide Substrates
Authors: Reszka Przemysław , Schulz Riad , Methling Karen , Lalk Michael , Bednarski Patrick J. 
Published: November 2009

AbstractThe synthesis, enzymatic evaluation, and molecular modeling studies of new fluorogenic tetrapeptide‐based substrates selective for caspase 8, having the general structure Ac‐IETD–AXX, are described. Various fluorescent reporter groups (AXX), i.e., 3‐ and 4‐substituted coumarins and quinolin‐2(1H)‐ones were synthesized by von Pechmann condensation. They were subsequently coupled with the caspase‐8‐selective tetrapeptide Ac‐IETD‐OH under newly developed synthetic conditions to give the desired substrates in good yields and in high enantiomeric purity. Based on KM and Vmax values, the new compounds proved to be excellent substrates for recombinant human caspase 8. In contrast, the KM values for the same compounds as substrates for human caspase 3 were approximately 10–20‐fold higher. Molecular modeling studies based on the X‐ray crystal structures of both human caspases 3 and 8 revealed that there is sufficient room within both active sites to accommodate substrates with moderately bulky substituents in the 3‐ and 4‐positions of the fluorogenic coumarins and quinolin‐2(1H)‐ones. Automated docking of the substrates into the active sites of both human caspases 3 and 8 with the program AutoDock 3 gave structures similar to the published crystallographic structures for the same tetrapeptide bound to caspase 8 in the form of an irreversible inhibitor. The calculated binding energies for the new substrates to either caspase 3 or 8 showed little difference between the substrates, consistent with the KM data. In addition, the calculated binding energies (ΔG) to caspase 8 were considerably more negative than those to caspase 3, also consistent with the KM data. A possible molecular interaction that might explain the selectivity of the IETD tetrapeptide motif for caspase 8 over caspase 3 is discussed.
Characteristics associated with early and late melanoma metastases
Authors: Brauer Jeremy A. , Wriston Cooper C. , Troxel Andrea B. , Elenitsas Rosalie , Shin Daniel B. , Guerry DuPont , Ming Michael E. 
Published: November 2009

AbstractBACKGROUND:Differences in risk factors for metastases at different time intervals after treatment have been described in several malignancies; however, to the authors' knowledge, no extensive study examining this issue in melanoma has been conducted to date.METHODS:The authors performed a nested case‐control study of patients with melanoma who presented with only local disease. Patients in the case group included 549 patients who developed metastases ≥6 months after surgery. Of these, 320 patients developed metastasis within 3 years after undergoing definitive surgery (early metastases [EM]), and 70 patients developed metastasis ≥8 years after undergoing definitive surgery (late metastases [LM]). For each case, a control patient was chosen who had melanoma but who did not develop metastases in the same interval. Univariate and conditional multivariate logistic regression were used in the analysis of 34 clinical and tumor characteristics.RESULTS:Multivariate analysis confirmed previously established risk factors for metastases, such as increasing tumor thickness. In addition, the authors discovered that a personal history of nonmelanoma skin cancer (P = .006) and a history of cancer other than skin cancer (P = .020) also were associated with metastasis. In comparing the 320 EM patients with the 70 LM patients, EM patients were more likely to have thicker lesions (P < .001), ulcerated lesions (P = .016), and a history of nonmelanoma skin cancer (P = .024).CONCLUSIONS:In this study, 2 potentially novel risk factors for melanoma metastases were identified, and different profiles of risk factors were constructed for EM versus LM. These differences may be important in future risk identification and stratification for clinical trials and for the management and treatment of patients with melanoma. Cancer 2010. © 2010 American Cancer Society.
Phase 2 trial of combination thalidomide plus temozolomide in patients with metastatic malignant melanoma: Southwest Oncology Group S0508
Authors: Clark Joseph I. , Moon James , Hutchins Laura F. , Sosman Jeffrey A. , Kast W. Martin , Da Silva Diane M. , Liu P. Y. , Thompson John A. , Flaherty Lawrence E. , Sondak Vernon K. 
Published: November 2009

AbstractBACKGROUND:In limited institution phase 2 studies, thalidomide and temozolomide has yielded response rates (RRs) up to 32% for advanced melanoma, leading to the use of this combination as “standard” by some. We conducted a multicenter phase 2 trial to better define the clinical efficacy of thalidomide and temozolomide and the immune modulatory effects of thalidomide, when combined with temozolomide, in patients with metastatic melanoma.METHODS:Patients must have had stage IV cutaneous melanoma, no active brain metastases, Zubrod PS 0‐1, up to 1 prior systemic therapy excluding thalidomide, temozolomide, or dacarbazine, adequate organ function, and given informed consent. The primary endpoint was 6‐month progression‐free survival (PFS). Secondary endpoints included overall survival (OS), RR, toxicities, and assessment of relationships between biomarkers and clinical outcomes. Patients received thalidomide (200 mg/d escalated to 400 mg/d for patients <70, or 100 mg/d escalated to 250 mg/d for patients ≥70) plus temozolomide (75 mg/m2/d × 6 weeks, and then 2 weeks rest).RESULTS:Sixty‐four patients were enrolled; 2 refused treatment. The 6‐month PFS was 15% (95% confidence interval [CI], 6%‐23%), the 1‐year OS was 35% (95% CI, 24%‐47%), and the RR was 13% (95% CI, 5%‐25%), all partial. One treatment‐related death occurred from myocardial infarction; 3 other grade 4 events occurred, including pulmonary embolism, neutropenia, and central nervous system (CNS) ischemia. There was no significant correlation between biomarkers and PFS or OS.CONCLUSIONS:This combination of thalidomide and temozolomide does not appear to have a clinical benefit that exceeds dacarbazine alone. We would not recommend it further for phase 3 trials or for standard community use. Cancer 2010. © 2010 American Cancer Society.
Evaluation of the breathworks mindfulness‐based pain management programme: effects on well‐being and multiple measures of mindfulness
Authors: Cusens Bryany , Duggan Geoffrey B. , Thorne Kirsty , Burch Vidyamala 
Published: November 2009

AbstractTwo studies of a mindfulness training programme are presented. Study 1 reports on a pilot investigation of the impact on well‐being of the Breathworks mindfulness‐based pain management programme. Significant positive change was found on self‐report measures of depression, outlook, catastrophizing and pain self‐efficacy in the Intervention Group, but not the Comparison Group. Particularly large effects were found for pain acceptance. These results support the short‐term efficacy of the Breathworks programme and reinforce the importance of acceptance for positive outcome with chronic pain patients. Study 2 investigated alterations in mindfulness following participation in the Breathworks programme. Subjective and non‐subjective measures of mindfulness were used. Scores on the Mindful Attention Awareness Scale were significantly higher at Time 2 in the Intervention Group, but not in the Comparison Group. There was no change on a measure of sustained attention. Results from an Implicit Association Test provided some support for an increased awareness of positive stimuli, following the intervention. These results are discussed with reference to the mechanisms of mindfulness. Copyright © 2009 John Wiley & Sons, Ltd.Key Practitioner Message:

Evidence supporting the efficacy of Breathworks for well‐being.
Mindful Attention Awareness Scale scores improved following mindfulness training.
Mindfulness may increase awareness of pleasant affect.
No change found on a measure of attention.
Shame as a traumatic memory
Authors: Matos Marcela , Pinto‐Gouveia José 
Published: November 2009

AbstractBackground: This study explores the premise that shame episodes can have the properties of traumatic memories, involving intrusions, flashbacks, strong emotional avoidance, hyper arousal, fragmented states of mind and dissociation.Method: A battery of self‐report questionnaires was used to assess shame, shame traumatic memory and depression in 811 participants from general population (481 undergraduate students and 330 subjects from normal population).Results: Results show that early shame experiences do indeed reveal traumatic memory characteristics. Moreover, these experiences are associated with current feelings of internal and external shame in adulthood. We also found that current shame and depression are significantly related. Key to our findings is that those individuals whose shame memories display more traumatic characteristics show more depressive symptoms. A moderator analysis suggested an effect of shame traumatic memory on the relationship between shame and depression.Limitations: The transversal nature of our study design, the use of self‐reports questionnaires, the possibility of selective memories in participants' retrospective reports and the use of a general community sample, are some methodological limitations that should be considered in our investigation.Conclusion: Our study presents novel perspectives on the nature of shame and its relation to psychopathology, empirically supporting the proposal that shame memories have traumatic memory characteristics, that not only affect shame in adulthood but also seem to moderate the impact of shame on depression. Therefore, these considerations emphasize the importance of assessing and intervening on shame memories in a therapeutic context. Copyright © 2009 John Wiley & Sons, Ltd.Key Practitioner Message:

Early shame experiences reveal traumatic memory characteristics and are related to current shame and to psychopathology.
Individuals whose shame memories have more traumatic characteristics are those who show more depressive symptoms.
Shame traumatic memories moderate the relationship between shame and depression, hence to the same shame, individuals who experienced shame as more traumatic are the ones who show more depressive symptoms.
Therapy for shame‐based problems needs to incorporate strategies to assess and address individuals shame traumatic memories.
Does illness attribution affect treatment assignment in depression?
Authors: Schweizer Susanne , Peeters Frenk , Huibers Marcus , Roelofs Jeffrey , van Os Jim , Arntz Arnoud 
Published: November 2009

AbstractObjective: Little is known about depressed individuals' illness attributions and how these influence treatment assignment in clinical practice. The aim of the present study was to examine whether illness attribution across the domains of intraindividual, interpersonal and biological reasons was associated with treatment assignment in a naturalistic treatment setting.Method: Illness attribution was assessed with the Reasons for Depression Questionnaire in 221 depressed individuals. Participants were assigned to either cognitive–behavioural therapy (CBT), interpersonal therapy (IPT) or psychopharmacological treatment (PHT).Results: Depressed individuals who strongly attributed their illness to intraindividual factors were more likely to be assigned to CBT, and depressed individuals attributing their depression to biological reasons were more likely to receive in PHT. In contrast, interpersonal illness attribution was not associated with treatment assignment.Conclusions: Illness attribution influences treatment assignment to CBT and PHT. However, factors other than illness attribution for depression affect a treatment choice of IPT. Copyright © 2009 John Wiley & Sons, Ltd.Key Practitioner Message:

Intraindividual and biological illness attributions affected depressed individuals' treatment assignment, making these individuals more likely to receive CBT and PHT, respectively.
Interpersonal illness attribution was not found to be associated with treatment assignment.
There were significant gender differences in illness attribution. Men were more likely to endorse in achievement‐related causes, and women attributed their depressive illnesses to more interpersonal reasons (i.e., relationship, childhood and intimacy).
The Green Onion: a corporate environmental strategy framework
Authors: Valentine Scott Victor 
Published: November 2009

AbstractSince the 1990s, there has been a proliferation of research exploring the benefits of proactive corporate environmental management initiatives. Unfortunately, the absence of a comprehensive, strategic planning framework relegates much of this valuable research to a study of good ideas for making money while operating more sustainably. This paper presents a framework for guiding corporate environmental strategy to bring order to existing observations and allow social scientists to begin the process of ‘orderly control and prediction’. The research is based on modified grounded theory and an extensive literature review pertaining to the benefits of corporate environmental management. The framework has been named the ‘Green Onion’ to highlight the multiple strategic layers of influence uncovered and the importance of retaining resilient outer layers (i.e., stakeholder management) to protect the highly potent core of functional environmental management initiatives (i.e., cost saving initiatives). Copyright © 2009 John Wiley & Sons, Ltd and ERP Environment.
Fusion of uniluminal vascular spheroids: A model for assembly of blood vessels
Authors: Fleming Paul A. , Argraves W. Scott , Gentile Carmine , Neagu Adrian , Forgacs Gabor , Drake Christopher J. 
Published: November 2009

AbstractWe evaluated the self‐assembly properties of uniluminal vascular spheroids having outer layers of vascular smooth muscle cells and a contiguous inner layer of endothelial cells lining a central lumen. We showed that while pairs of uniluminal vascular spheroids suspended in culture medium fused to form a larger diameter spheroidal structure, spheroids in collagen hydrogels formed elongated structures. These findings highlight the potential use of uniluminal vascular spheroids as modules to engineer blood vessels. We also demonstrate that uniluminal vascular spheroid fusion conforms to models describing the coalescence of liquid drops. Furthermore, the fusion of uniluminal vascular spheroids in vitro closely resembled the in vivo process by which the descending aorta forms from the fusion of the paired dorsal aortae during embryonic development. Together, the findings indicate that tissue liquidity underlies uniluminal vascular spheroid fusion and that in vivo anastomosis of blood vessels may involve a similar mechanism. Developmental Dynamics 239:398–406, 2010. © 2009 Wiley‐Liss, Inc.
Sinusoid development and morphogenesis may be stimulated by VEGF‐Flk‐1 signaling during fetal mouse liver development
Authors: Sugiyama Yoshinori , Takabe Yurie , Nakakura Takashi , Tanaka Shigeyasu , Koike Toru , Shiojiri Nobuyoshi 
Published: November 2009

AbstractEarly morphogenesis of hepatic sinusoids was histochemically and experimentally analyzed, and the importance of VEGF‐Flk‐1 signaling in the vascular development was examined during murine liver organogenesis. FITC‐gelatin injection experiments into young murine fetuses demonstrated that all primitive sinusoidal structures were confluent with portal and central veins, suggesting that hepatic vessel development may occur via angiogenesis. At 12.5–14.5 days of gestation, VEGF receptors designated Flk‐1, especially their mature form, were highly expressed in endothelial cells of primitive sinusoidal structures and highly phosphorylated on their tyrosine residues. At the same time, VEGF was also detected in hepatoblasts/hepatocytes, hemopoietic cells, and megakaryocytes of the whole liver parenchyma. Furthermore, the addition of VEGF to E12.5 liver cell cultures significantly induced the growth and branching morphogenesis of sinusoidal endothelial cells. Therefore, VEGF‐Flk‐1 signaling may play an important role in the growth and morphogenesis of primitive sinusoids during fetal liver development. Developmental Dynamics 239:386–397, 2010. © 2009 Wiley‐Liss, Inc.
A migratory role for EphrinB ligands in avian epicardial mesothelial cells
Authors: Wengerhoff Sonia M. , Weiss Amy R. , Dwyer Kathryn L. , Dettman Robert W. 
Published: November 2009

AbstractLittle is known about the molecules that mediate the attachment of proepicardial cells to the heart. Ephrins are cell surface ligands for Eph tyrosine kinase receptors, molecules known to play a role in cell adhesion and migration. Here, we detected EphrinB ligands in proepicardial and epicardial mesothelial cells (EMCs) using reverse transcriptase‐polymerase chain reaction, immunoblotting, immunolocalization, and EphB‐Fc binding. Aggregated EphB‐Fc fragments clustered ephrinB1 ligands on living EMCs indicating that they are cell surface expressed. In vitro assays demonstrated that ephrinB ligands participate in EMC migration but not cell adhesion. Localization studies in hearts at Hamburger and Hamilton stage 30 and older revealed that ephrinB1 is expressed in the epicardium and subepicardial mesenchyme of the atrioventricular sulcus. EMCs treated with platelet‐derived growth factor‐BB expressed smooth muscle markers but not ephrinB1. Our study supports an early role for ephrinB ligands for migration of epicardial cells and a later role in perivascular fibroblasts of coronary vessels in the atrioventricular sulcus. Developmental Dynamics 239:598–609, 2010. © 2009 Wiley‐Liss, Inc.
Spatial (Tbata) expression in mature medullary thymic epithelial cells
Authors: Saade Murielle , Irla Magali , Yammine Miriam , Boulanger Nicolas , Victorero Geneviève , Vincentelli Renaud , Penninger Josef M. , Holländer Georg A. , Chauvet Sophie , Nguyen Catherine 
Published: November 2009

AbstractThe Spatial gene is expressed in highly polarized cell types such as testis germ cells, brain neurons and thymic epithelial cells (TEC). Its expression was documented in testis and brain but poorly characterized in thymus. Here, we characterize for the first time Spatial‐expressing TEC throughout ontogeny and adult mouse thymus. Spatial is expressed in thymic‐fated domain by embryonic day E10.5 and persists in subcapsular, cortical, medullary epithelial cells and in MTS24+ progenitor TEC. Using mouse strains in which thymocyte development is blocked at various stages, we show that Spatial expression is independent of thymocyte‐derived signals during thymus organogenesis. Analyses on purified thymic cell subsets show that Spatial short isoforms are expressed in cortical TEC (cTEC) and mature medullary TEC (mTEC). Spatial long isoforms were detected in the same TEC population. Spatial presents a nuclear distribution specific to mature mTEC expressing UEA1 and Aire. Aire‐ and RANKL‐deficient mice revealed that Spatial expression is drastically reduced in the thymus of these mutants. These findings reveal a critical function of Aire in regulating Spatial expression, which is compatible with promiscuous Spatial gene expression.