bluuebunny/crossref_metadata_2025_split huggingface dataset:
A blind deconvolution approach to high-resolution mapping of transcription factor binding sites from ChIP-seq data
Authors: Lun Desmond S , Sherrid Ashley , Weiner Brian , Sherman David R , Galagan James E 
Published: December 2009

AbstractWe present CSDeconv, a computational method that determines locations of transcription factor binding from ChIP-seq data. CSDeconv differs from prior methods in that it uses a blind deconvolution approach that allows closely-spaced binding sites to be called accurately. We apply CSDeconv to novel ChIP-seq data for DosR binding in Mycobacterium tuberculosis and to existing data for GABP in humans and show that it can discriminate binding sites separated by as few as 40 bp.
The Rehabilitation Needs of Adult Siblings of Persons With Traumatic Brain Injury: A Quantitative Investigation
Authors: Degeneffe Charles Edmund 
Published: December 2009

AbstractThis study examined the post-injury rehabilitation needs of 158 adult siblings of persons with traumatic brain injury (TBI) in the United States. The mean age of participants was 37.0 years (SD= 10.0 years), and their ages ranged from 19 to 72 years. A total of 120 (75.9%) of the participants were female. Participants completed the Family Needs Questionnaire (FNQ) (Kreutzer, 1988), which measures the unique needs of families following TBI. The FNQ counts the types of needs rated as important and the frequency in which those needs have been met. Data were analysed via hierarchical multiple regression analysis with the number of met needs set as the outcome variable. A total of 15 predictor variables assessed direct and indirect markers of caregiving related stresses corresponding to position in the Pearlin stress process model. Participants indicated that health information was the most important need while needs for involvement with care were the most often met. Results also indicated that participant feelings of more restrictions in valued family activities and perceptions of greater cognitive-behavioural impairments with their injured siblings were associated with lower number of met rehabilitation needs. Participants reported that the use of effective coping behaviours and access to social support were associated with higher numbers of met rehabilitation needs. Findings suggest adult siblings share similar needs to parents and spouses (i.e., the most common primary caregivers) and require professional attention and support following TBI.
Brain Tumor Signs and Symptoms: Analysis of Primary Health Care Records From the UKCCS
Authors: Ansell Pat , Johnston Tom , Simpson Jill , Crouch Simon , Roman Eve , Picton Susan 
Published: December 2009

OBJECTIVE: To compare the frequency of brain tumor signs and symptoms in children with and without brain tumors.
METHODS: This was a UK population-based retrospective analysis of primary care records. Participants were 195 children (1–14 years) newly diagnosed with brain tumors and 285 controls matched by age, gender, and region. Comparisons included total number of prediagnosis consultations, number with ≥1 symptom suggestive of a brain tumor, total number of symptoms, number of different symptoms, and number of visits with specific combinations of symptoms.
RESULTS: On average, cases consulted more often than controls between birth and diagnosis/pseudodiagnosis with brain tumor signs and symptoms. Their consultation rate with ≥1 suggestive symptom escalated in the 2 years before diagnosis. Symptom prevalence was higher among cases than controls, a relative difference of 3.29 times as many consultations with ≥1 suggestive symptom (95% confidence interval [CI]: 2.82–3.83) and 7.01 as many with more than 1 (95% CI: 5.38–9.13). In each 6-month period in the 4 years before diagnosis, cases had at least twice as many consultations with ≥1 suggestive symptom (20.81 times as many in the 6 months before diagnosis [95% CI: 14.29–30.30]) and 2–3 times more records of suggestive symptoms (28.35 times more in the 6 months before diagnosis [95% CI: 19.05–42.19]). Symptoms rarely or not observed among control children included head tilt, odd head movements, odd posture, back or neck stiffness, and unsteadiness without obvious cause.
CONCLUSION Key to identifying the 1 child among many who merits prompt investigation is recognition of unusual symptoms, or specific symptom patterns.
LASIK Flap Buttonhole Treated Immediately by PRK With Mitomycin C
Authors: Kymionis George D. , Portaliou Dimitra M. , Karavitaki Alexandra E. , Krasia Maria S. , Kontadakis Georgios A. , Stratos Aimilianos A. , Yoo Sonia H. 
Published: December 2009

PURPOSE:
To describe the visual outcomes of three patients who had LASIK flap buttonhole and were treated immediately with photorefractive keratectomy (PRK) and topical mitomycin C (MMC) 0.02%.

METHODS:

Three patients underwent bilateral LASIK with the SCHWIND Carriazo-Pendular 90-
µ
m head microkeratome. In all three cases, a buttonhole flap occurred in the left eye. The flap was repositioned and phototherapeutic keratectomy for 50
µ
m was used for epithelial removal while immediate PRK with MMC was performed to treat the buttonhole flap.

RESULTS:
Three months after the procedure, uncorrected distance visual acuity and corrected distance visual acuity were 20/20 with regular topographic findings.

CONCLUSIONS:

Using PRK with MMC immediately after the occurrence of a LASIK flap buttonhole may be an effective treatment. [
J Refract Surg
. 2010;26:225–228.]
Determining Spherocylindrical Correction Using Four Different Wavefront Error Analysis Methods: Comparison to Manifest Refraction
Authors: Campbell Charles E. 
Published: December 2009

Purpose:
To evaluate methods that determine refractive correction from wavefront data that best matches the manifest refraction.

Methods:
Wavefront data in the form of Zernike polynomial coefficients from several VISX US Investigational Device Exemption studies were used to calculate the spherocylindrical distance correction using four methods: full pupil second order, central curvature, reduced aperture, and 4-mm pupil. The data were analyzed in two groups, preoperative (3686 measurements, 882 eyes) and 6-month postoperative (2570 measurements, 797 eyes). Differences were formed between values found with each method and the manifest refractive values for the spherical equivalent (dSE), cylinder power (dCc), and axis power (dAc). The wavefront spherical equivalent values were corrected for chromatic shift, lane length, and vertex.

Results:

Values derived using the reduced aperture and 4-mm pupil methods corresponded with manifest spherical equivalent values for both preoperative (
x̄
=−0.03 diopters [D],
x̄
=0.95 D) and postoperative (
x̄
=−0.36 D,
x̄
=0.65 D) data. The full pupil second order method results corresponded best with manifest data for the astigmatic variables for both preoperative (
x̄ dCc
=0.15 D, σ
dCc
=0.31 D,
x̄ dAc
= 0.02 D, σ
dAc
=0.22 D) and postoperative data (
x̄ dCc
=0.25 D, σ
dCc
=0.33 D,
x̄ dAc
=0.01 D, σ
dAc
=0.21 D). However, all methods did well on these variables.

Conclusions:
The Zernike coefficient set as measured best calculates the astigmatic correction. The Zernike coefficient set resized to a 4-mm pupil is best in determining spherical equivalent.
US FDA Clinical Trial of the Tetraflex Potentially Accommodating IOL: Comparison to Concurrent Age-Matched Monofocal Controls
Authors: Sanders Donald R. , Sanders Monica L. ,   Tetraflex Presbyopic IOL Study Group
Published: December 2009

Purpose:To assess the efficacy of the Tetraflex (Lenstec Inc) intraocular lens (IOL) to provide enhanced near reading ability and spectacle independence relative to a monofocal control IOL in bilaterally implanted eyes tested binocularly.Methods:A prospective, age-matched, non-randomized US Food and Drug Administration clinical trial of 255 Tetraflex and 101 monofocal IOL control patients was performed. To date, 239 Tetraflex and 96 control patients were examined at 12 months postoperatively.Results:At 12 months postoperative, the Tetraflex patients read better than the controls at print sizes of 20/80 (P=.04), 20/63 (P=.01), 20/50 (P<.001), 20/40 (P=.001), 20/32 (P<.001), and 20/25 (P=.001). The proportion of patients reading at a speed of ≥80 words per minute was significantly higher with the Tetraflex IOL (P=.003). Ninety-six percent of Tetraflex patients reported never wearing glasses for distance compared with 80% of control patients (P<.001). Seventy-five percent of the Tetraflex patients reported near spectacle wear either never or only occasionally for small print and/or dim light (21% never) compared with 46% of control patients (P<.001) (9% never). Near add power requirement for corrected near visual acuity was less in the Tetraflex group (P<.001); 28% of Tetraflex patients required ≤1.25 diopters of near add, compared to only 7% of control patients. Spectacle independence, as measured by the proportion of patients with uncorrected distance visual acuity of 20/25 or better and various degrees of uncorrected near visual acuity, was also significantly better (P<.001) as was distance-corrected near visual acuity (P<.001).Conclusions:The results support the efficacy of the Tetraflex IOL to provide enhanced near reading ability and spectacle independence relative to a monofocal IOL control.
Pupil Size, White-to-White Corneal Diameter, and Anterior Chamber Depth in Patients with Myopia
Authors: Alfonso José F. , Ferrer-Blasco Teresa , González-Méijome José M. , García-Manjarres M. , Peixoto-de-Matos Sofia C. , Montés-Micó Robert 
Published: December 2009

Purpose:
To evaluate anatomical parameters in a population of patients with myopia.

Methods:
Nine hundred sixty-four myopic eyes (−3.00 to −20.00 diopters [D] spherical equivalent refraction) were evaluated to measure mesopic and photopic pupil size with an infrared pupillometer; anterior chamber depth and white-to-white corneal diameter were obtained with Orbscan II (Bausch & Lomb). Correlation analysis was performed to evaluate the relationships among anatomical parameters of the anterior segment of the eye.

Results:

Average change in pupil size between mesopic and photopic conditions shows a uniform gap of 1.5 mm in patients aged 18 to 62 years with a slight insignificant trend to decrease with age. Photopic and mesopic pupil size were highly correlated (r=0.694,
P
<.001) and the difference between both measures was positively correlated with mesopic pupil size (r=0.207,
P
<.001) and inversely correlated with photopic pupil size (r=0.561,
P
<.001). Anterior chamber depth and white-to-white corneal diameter were positively correlated (r=0.389,
P
<.001). White-to-white corneal diameter and anterior chamber depth were not correlated with age (r=−0.096,
P
<.001) or anterior chamber depth (r=−0.183,
P
<.001–0.183) as a function of age.

Conclusions:
Average difference between photopic and mesopic pupil size remained constant across the range of ages included in this cohort. A positive correlation was noted between anterior segment dimensions, and anterior chamber depth decreased with age.
Copy number and sequence variants implicate APBA2 as an autism candidate gene
Authors: Babatz Timothy D. , Kumar Ravinesh A. , Sudi Jyotsna , Dobyns William B. , Christian Susan L. 
Published: December 2009

AbstractWe recently reported an autistic proband and affected sibling with maternally inherited microduplications within the 15q13.1 and 15q13.3 regions that contain a total of 4 genes. The amyloid precursor protein‐binding protein A2 (APBA2) gene is located within the 15q13.1 duplication and encodes a neuronal adaptor protein essential to synaptic transmission that interacts directly with NRXN1 at the presynaptic membrane. We interpreted this as evidence for a putative role of APBA2 in autism as larger maternal duplications of 15q11‐q13 are the most common known cause of autism. We therefore resequenced 512 subjects with autism spectrum disorder (ASD) and 463 controls, and identified 7 novel nonsynonymous coding variants in ASD subjects compared with 4 in controls. Five of the seven variants in the ASD group were predicted to affect protein function, alter residues conserved across 18 species, or both. All of the variants for which parental DNA was available were inherited. We also found two different nonsynonymous variants in two siblings with autism: (1) a paternally inherited heterozygous 6 bp deletion and (2) a maternally inherited heterozygous missense mutation, the latter also found in a single control. These results indicate compound heterozygous mutations of APBA2 in this autism sibship. The co‐occurrence of two nonsynonymous mutations in both affected siblings in a single family, each transmitted from a different unaffected parent, suggest a role for APBA2 mutations in rare individuals with ASD.
Dose‐dependent actions of curcumin in experimentally induced myocardial necrosis: a biochemical, histopathological, and electron microscopic evidence
Authors: Tanwar Vineeta , Sachdeva Jaspreet , Kishore Kamal , Mittal Rajan , Nag Tapas Chandra , Ray Ruma , Kumari Santosh , Arya Dharamvir Singh 
Published: December 2009

AbstractCurcumin, an active component of turmeric, is a well‐known antioxidant due to its reactive oxygen species (ROS) scavenging property. However, some in vitro studies have suggested that curcumin induces generation of ROS at higher doses and thus exerts pro‐oxidant effect. We demonstrate, for the first time, the dose‐dependent effects of curcumin in isoprenaline‐induced model of myocardial necrosis in rats. The animals were assigned to control, isoprenaline and three curcumin treatment groups. Curcumin (100, 200, and 400 mg/kg) and vehicle (dimethyl sulfoxide) were administrated orally for 15 days and isoprenaline (85 mg/kg, s.c.) was given to curcumin treated and isoprenaline group on 13th and 14th day, respectively. Thereafter, on 15th day, the animals were sacrificed for biochemical analysis along with histopathological and ultrastructural examination. There was an increase in glutathione, superoxide dismutase (SOD), creatine kinase‐MB (CK‐MB) and lactate dehydrogenase (LDH) levels, decrease in thiobarbituric acid reactive substances (TBARS), and preservation of myocardial architecture in the curcumin (100 and 200 mg/kg) treated groups. However, at 400 mg/kg dose there was ineffectual protection against isoprenaline‐induced myocardial damage. Instead, there was significant lipid peroxidation as evident by increased levels of TBARS (93.87 ± 9.93, p < 0.0001) and decrease in CK‐MB (206.32 ± 13.54, p < 0.0001) and LDH (134.26 ± 9.13, p < 0.01) as compared to the two lower doses. Hence, it can be concluded that curcumin augments endogenous antioxidant system at lower doses but mediates ROS induction at higher concentration leading to myocardial damage. Copyright © 2009 John Wiley & Sons, Ltd.
Wandering coronary stenoses: Adrenaline‐induced coronary artery spasm in a patient resuscitated from cardiac arrest
Authors: Battaglia Salvatore , Nammas Wail 
Published: December 2009

AbstractA 68‐year‐old ex‐smoker man with history of allergy, presented to the emergency department with progressive dyspnea one hour following self‐medication with aspirin for troublesome headache. Examination revealed diffuse sibilant rhonchi over both lungs. Electrocardiogram showed signs of ischemia. In the intensive care unit, he received bronchodilators, nitroglycerin, and aspirin. Bronchospasm increased, and then the patient was shocked, and developed cardiac arrest. After resuscitation, he was kept on mechanical ventilation and adrenaline infusion. He was sceduled for coronary angiography. The left system demonstrated stenosis of the mid‐segment of the left anterior descending artery (LAD), which was totally occluded distally, stenosis of the left circumflex (LCx) with a mild plaque in its marginal branch. The right system demonstrated stenosis of the mid‐segment of the right coronary artery (RCA), with diffusely diseased posterior descending artery (PDA) and posterolateral left ventricular branch (PLLV). Successful direct stenting was performed to the RCA. Angiography demonstrated worsening of the distal stenosis in the PLLV and complete occlusion of the PDA. Balloon dilatation of the PLLV was adequate, but dilatation of the PDA failed. Repeat angiography of the left system revealed an occluded LCx with critical stenosis of its marginal branch; nevertheless, the LAD was as before. Balloon dilatation of the distal LAD was attempted without improvement, yet, angiography therein, demonstrated “migration” of the stenoses in the LCx. The procedure was halted, adrenaline infusion discontinued, and an intra‐aortic balloon pump inserted. The patient was discharged one day later. Follow‐up angiography 6 months later demonstrated mild atherosclerotic coronary irregularities. © 2010 Wiley‐Liss, Inc.
Feasibility of percutaneous transcatheter mitral valve repair with the MitraClip® system using conscious sedation
Authors: Ussia Gian Paolo , Barbanti Marco , Tamburino Corrado 
Published: December 2009

AbstractWe report the first human case of percutaneous transcatheter mitral valve repair with the MitraClip® system under conscious sedation in a patient with severe mitral regurgitation. The procedure was performed using the standard MitraClip® technique, in the catheterization laboratory, with transesophageal echocardiographic and fluoroscopic guidance. Transesophageal echocardiography is the primary imaging modality of the MitraClip procedure, which usually necessitates general anesthesia to guarantee complete immobilization of the patient allowing precise delivery of the MitraClip® device. We successfully performed the procedure under deep sedation, using remifentanil and local anesthesia in a patient who had serious contraindications to general anesthesia. The success of this procedure suggests that percutaneous transcatheter mitral repair with the MitraClip® system can be safely realized using conscious sedation. Conscious sedation may represent a convenient alternative approach for selected patients who undergo this innovative procedure. © 2010 Wiley‐Liss, Inc.
Synthesis and Optical Properties of Europium‐Complex‐Doped Inorganic/Organic Hybrid Materials Built from Oxo–Hydroxo Organotin Nano Building Blocks
Authors: Fan Wei‐Qiang , Feng Jing , Song Shu‐Yan , Lei Yong‐Qian , Zheng Guo‐Li , Zhang Hong‐Jie 
Published: December 2009

AbstractHybrid materials doped with novel europium complexes were synthesized using PMMA‐co‐Sn12Clusters (copolymers from oxohydroxo‐organotin dimethacrylate and methylmethacrylate) as the matrix material. Two types of hybrid materials were obtained: the physically doped product, PMMA‐co‐Sn12Cluster/Eu(TTA)3phen, and the grafted product, PMMA‐co‐Sn12Cluster‐co‐[EuAA(TTA)2phen] (TTA=2‐thenoyltrifluoroacetone, phen=phenanthroline and AA=acrylic acid). The hybrid materials exhibited characteristic luminescence of the Eu3+ ions, and also showed relative especial optical properties compared with samples just using PMMA as the matrix material. The PMMA‐co‐Sn12Cluster matrix exhibited a high physical doping quantity of [Eu(TTA)3phen], which can be attributed to the special structure of this kind of hybrid material. GPC (gel‐permeation chromatography), TGA (thermogravimetric analysis), SEM, 1H NMR, ICP (inductively coupled plasma), 119Sn NMR, FTIR, and diffuse reflectance techniques were employed to characterize the structures and properties of these hybrid materials.
Regiocontrolled Synthesis of Ethene‐Bridged para‐Phenylene Oligomers Based on PtII‐ and RuII‐Catalyzed Aromatization
Authors: Chen Tse‐An , Lee Te‐Ju , Lin Ming‐Yuan , Sohel Shariar M. A. , Diau Eric Wei‐Guang , Lush Shie‐Fu , Liu Rai‐Shung 
Published: December 2009

AbstractWe report the regiocontrolled syntheses of ethene‐bridged para‐phenylene oligomers in three distinct classes by using PtII‐ and RuII‐catalyzed aromatization. This synthetic approach has been developed based on twofold aromatization of the 1‐aryl‐2‐alkynylbenzene functionality, which proceeds by distinct regioselectivity for platinum and ruthenium catalysts. Variable‐temperature NMR spectra provide evidence that large arrays of these oligomers are prone to twist from planarity. The UV/Vis and photoluminescence (PL) spectra as well as the band gaps of these regularly growing arrays show a pattern of extensive π conjugation with increasing array sizes, except for in one instance.
Evaluation of direct medical costs of hospitalization for febrile neutropenia
Authors: Lathia Nina , Mittmann Nicole , DeAngelis Carlo , Knowles Sandra , Cheung Matthew , Piliotis Eugenia , Shear Neil , Walker Scott 
Published: December 2009

AbstractBACKGROUND:Treatment of febrile neutropenia (FN) is costly, because it typically involves hospitalization. As cancer rates continue to increase, the number of patients suffering from FN will also increase, making it important to quantify the costs of treating this condition accurately and comprehensively.METHODS:A consecutive sample of patients admitted to an inpatient hematology/oncology ward at a tertiary care hospital for the treatment of chemotherapy‐induced FN was enrolled in this study. Patients were followed prospectively during hospitalization, and information on medical resource utilization including length of stay, medications, and laboratory and diagnostic tests was collected. Costs, extracted from hospital and provincial databases, were used to calculate the overall cost per FN episode, from the hospital perspective.RESULTS:Fifty‐one episodes of FN that occurred in 46 patients were included in the study. Approximately 52% of these episodes occurred in women, and 65% of these episodes occurred in patients with hematologic malignancies. The mean ± standard deviation age of patients was 60.3 ± 13.4 years. The mean length of stay per episode was 6.8 ± 4.9 days. The mean overall cost per episode was 6324 ± 4783 in 2007 Canadian dollars.CONCLUSIONS:Hospitalization for the treatment of FN is expensive. The results of this study could be used in future economic evaluations of preventive measures and treatments for FN, including primary prophylactic administration of hematopoietic growth factors and outpatient treatment of this condition. Cancer 2010. © 2009 American Cancer Society.