bluuebunny/crossref_metadata_2025_split huggingface dataset:
A critical review of the reporting of reflexive thematic analysis in Health Promotion International
Authors: Braun Virginia , Clarke Victoria 
Published: May 2024

Abstract
Using the concept of methodological congruence—where the different elements of a study ‘fit’ together—we explore both problematic and good practice in (reflexive) thematic analysis (TA) as reported in Health Promotion International (HPI). Aligning with the importance we place on ‘owning your perspectives’ we situate this exploration in relation to our understanding of the variation in approaches to TA and qualitative research more broadly. This contextualization is necessary for highlighting why we judge practices to be in/congruent, and to facilitate more knowing congruence in future research. We critically reviewed a ‘sample’ of 31 papers published in HPI between 2010 and 2023 citing Braun and Clarke as reference for TA. We overview a range of problematic and good features of the use of TA in HPI, before focusing on two domains that seemed to present key challenges: theory and themes. Methodological incongruence can occur when postpositivist values and practices unwittingly creep into ostensibly non-positivist TA; we encourage thoughtfully and what we term ‘knowing’ consideration of theory, and quality practices and criteria. Methodological incongruence can also occur through mismatched conceptualizations of themes—notably, the use of ‘topic summaries’ as themes for reflexive TA (and fragmented thematic structures with ‘thin’ themes). We provide examples from the reviewed papers to demonstrate good practice in researcher reflexivity, articulation of theoretical and methodological frameworks and congruent themes. However, mindful of power dynamics, we only discuss problematic practice in general terms, to protect author anonymity. To facilitate thoughtful, quality TA—of all kinds—we provide eight pointers for researchers (and reviewers) to guide quality practice, and facilitate the use of concepts, procedures and criteria that promote knowing methodological congruence.
Amphibians Exhibit Extremely High Hydric Costs of Respiration
Authors: Riddell Eric A , Burger Isabella J , Muñoz Martha M , Weaver Savannah J , Womack Molly C 
Published: May 2024

Synopsis
Terrestrial environments pose many challenges to organisms, but perhaps one of the greatest is the need to breathe while maintaining water balance. Breathing air requires thin, moist respiratory surfaces, and thus the conditions necessary for gas exchange are also responsible for high rates of water loss that lead to desiccation. Across the diversity of terrestrial life, water loss acts as a universal cost of gas exchange and thus imposes limits on respiration. Amphibians are known for being vulnerable to rapid desiccation, in part because they rely on thin, permeable skin for cutaneous respiration. Yet, we have a limited understanding of the relationship between water loss and gas exchange within and among amphibian species. In this study, we evaluated the hydric costs of respiration in amphibians using the transpiration ratio, which is defined as the ratio of water loss (mol H2O d−1) to gas uptake (mol O2 d−1). A high ratio suggests greater hydric costs relative to the amount of gas uptake. We compared the transpiration ratio of amphibians with that of other terrestrial organisms to determine whether amphibians had greater hydric costs of gas uptake relative to plants, insects, birds, and mammals. We also evaluated the effects of temperature, humidity, and body mass on the transpiration ratio both within and among amphibian species. We found that hydric costs of respiration in amphibians were two to four orders of magnitude higher than the hydric costs of plants, insects, birds, and mammals. We also discovered that larger amphibians had lower hydric costs than smaller amphibians, at both the species- and individual-level. Amphibians also reduced the hydric costs of respiration at warm temperatures, potentially reflecting adaptive strategies to avoid dehydration while also meeting the demands of higher metabolic rates. Our results suggest that cutaneous respiration is an inefficient mode of respiration that produces the highest hydric costs of respiration yet to be measured in terrestrial plants and animals. Yet, amphibians largely avoid these costs by selecting aquatic or moist environments, which may facilitate more independent evolution of water loss and gas exchange.
Period Relations Between the Betti–Whittaker Periods for GLn Under Duality
Authors: Chen Shih-Yu 
Published: May 2024

Abstract
In this paper, under some regularity conditions, we prove a period relation between the Betti–Whittaker periods associated to a regular algebraic cuspidal automorphic representation of $\operatorname{GL}_{n}({{\mathbb{A}}})$ and its contragredient. As a consequence, we prove the trivialness of the relative period associated to a regular algebraic cuspidal automorphic representation of $\operatorname{GL}_{2n}({{\mathbb{A}}})$ of orthogonal type, which implies the algebraicity of the ratios of successive critical $L$-values for $\textrm{GSpin}_{2n}^{*} \times \operatorname{GL}_{n^{\prime}}$ by the result of Harder and Raghuram.
Chronotropic Incompetence Among People With HIV Improves With Exercise Training in the Exercise for Healthy Aging Study
Authors: Durstenfeld Matthew S , Wilson Melissa P , Jankowski Catherine M , Ditzenberger Grace L , Longenecker Chris T , Erlandson Kristine M 
Published: May 2024

Abstract

Background
People with HIV (PWH) have lower exercise capacity than peers without HIV, which may be explained by chronotropic incompetence, the inability to increase heart rate during exercise.

Methods
The Exercise for Healthy Aging Study included adults aged 50 to 75 years with and without HIV. Participants completed 12 weeks of moderate-intensity exercise, before randomization to moderate or high intensity for 12 additional weeks. We compared adjusted heart rate reserve (AHRR; chronotropic incompetence &lt;80%) on cardiopulmonary exercise testing by HIV serostatus and change from baseline to 12 and 24 weeks using mixed effects models.

Results
Among 32 PWH and 37 controls (median age, 56 years; 7% female), 28% of PWH vs 11% of controls had chronotropic incompetence at baseline (P = .067). AHRR was lower among PWH (91% vs 101%; difference, 10%; 95% CI, 1.9%–18.9%; P = .02). At week 12, AHRR normalized among PWH (+8%; 95% CI, 4%–11%; P &lt; .001) and was sustained at week 24 (+5%; 95% CI, 1%–9%; P = .008) versus no change among controls (95% CI, −4% to 4%; P = .95; interaction P = .004). After 24 weeks of exercise, 15% of PWH and 10% of controls had chronotropic incompetence (P = .70).

Conclusions
Chronotropic incompetence contributes to reduced exercise capacity among PWH and improves with exercise training.
The efficacy of 1-allyloxy-4-propoxybenzene (3c{3,6}) against Varroa destructor mites in honey bee colonies from Maryland, USA
Authors: Cook Steven C , Johnson Josephine D , Rogers Curtis W , Sachs Ian E , Feng Xinyi , Plettner Erika 
Published: May 2024

Abstract
Varroa destructor Oud (Acari: Varroidae) is a harmful ectoparasite of Apis mellifera L. honey bees causing widespread colony losses in Europe and North America. To control populations of these mites, beekeepers have an arsenal of different treatments, including both chemical and nonchemical options. However, nonchemical treatments can be labor intensive, and Varroa has gained resistance to some conventional pesticides, and the use of other chemical treatments is restricted temporally (e.g., cannot be applied during periods of honey production). Thus, beekeepers require additional treatment options for controlling mite populations. The compound 1-allyloxy-4-propoxybenzene (3c{3,6}) is a diether previously shown to be a strong feeding deterrent against Lepidopteran larvae and a repellent against mosquitoes and showed promise as a novel acaricide from laboratory and early field trials. Here we test the effect of the compound, applied at 8 g/brood box on wooden release devices, on honey bees and Varroa in field honey bee colonies located in Maryland, USA, and using a thymol-based commercial product as a positive control. 3c{3,6} had minimal effect on honey bee colonies, but more tests are needed to determine whether it affected egg production by queens. Against Varroa3c{3,6} had an estimated efficacy of 78.5%, while the positive control thymol product showed an efficacy of 91.3%. 3c{3,6} is still in the development stage, and the dose or application method needs to be revisited.
Elevated nitrogen and co-evolution history with competitors shape the invasion process of Galinsoga quadriradiata
Authors: Zhang Wen-Gang , Song Xing-Jiang , Petri Laís , Liu Gang , Chen Xiao-Yan , Liu Rui-Ling , Huang Fang-Fang , Zou Jia-Bin , Zhu Zhi-Hong 
Published: May 2024

Abstract
Invasive plants usually experience population differentiation as they expand from their initial invasive range to the edge. Moreover, invasive plants usually encounter competitors which shared different co-evolutionary histories with them. These factors may lead to varying responses of invasive plant populations to elevated nitrogen deposition during expansion. However, this issue has received limited attention in prior research. To address these challenges, we conducted a greenhouse experiment to investigate how population differentiation of Galinsoga quadriradiata interacts with the presence of various competitors in response to increased nitrogen deposition. Competitor types (new or old that shared short or long co-evolutionary history with the invader, respectively) were set to compete with the invasive central and edge populations under different nitrogen addition treatments. Individuals from the central population of G. quadriradiata, originating from the initial invasion range, showed greater total mass, reproduction and interspecific competitiveness compared with the edge population. Nitrogen addition improved growth and reproductive performance in both populations, and the central population had a stronger response compared with the edge population. The performance of G. quadriradiata was inhibited more effectively by old competitors than new competitors. Our results indicate that population differentiation occurs in terms of growth and competitiveness during the range expansion of G. quadriradiata, with the central population exhibiting superior performance. Co-evolutionary history with competitors is considered unfavorable for invasive plants in this study. Our results highlight the combined effects of population differentiation in invasive species and their co-evolution history with competitors in the context of global change factors.
Systematic Implications of Palynomorph Diversity Using Microscopic Trends Among Asteraceous Flora From the Drylands of Baluchistan, Pakistan
Authors: Sadia Bibi , Ahmad Mushtaq , Ramadan Mohamed Fawzy , Zafar Muhammad , Sultana Shazia , Noor Wajia , Makhkamov Trobjon , Yuldashev Akramjon , Khaydarov Khislat , Pieroni Andrea 
Published: May 2024

Abstract
Pollen micromorphological traits with taxonomic implications are first reported from the study area for 50 Asteraceous taxa belonging to nine tribes. Cichorieae (21 taxa), Cardueae (11 taxa), Inuleae (six taxa), and Anthemideae (four taxa) are the leading tribes. The research included Cousinia haeckeliae, Himalaiella afghana, Pterachaenia stewartii (endemic to Afghanistan and Pakistan), and Xylanthemum macropodum (endemic to Baluchistan). Light and scanning electron microscopy were employed for the visualization of pollen photomicrographs. The data was analyzed statistically via SPSS, PAST, and Origin. Significant diagnostic qualitative and quantitative palynological traits were explored for discrimination down to the species level within the tribes. All the investigated taxa possessed radial symmetry, isopolarity, and monad form (characters for distinction at the subdivision level). The aperture types were trizonocolporate, tetrazonocolporate, and tricolporate with number position and character (NPC) formulas N3P4C5, N4P4C5, and N3P4C3. Goniotreme, peritreme, and ptychotreme types of amb were recognized. Echinate, echinate lophate, scabrate, and gemmate sculpturing were present with and without perforated surface patterns. Variations in the shapes in polar and equatorial views and lacuna shapes further assisted the separation of taxa. The observed shape classes were perprolate, prolate spheroidal, prolate, subprolate, oblate spheroidal, suboblate, and oblate. Principal component analysis, correlation, standard probability plots, and ridge line paired features plot for quantitative variables determined the positive correlation between the length and width of colpi in equatorial and polar view with polar axis and equatorial diameter and number of spines between colpi with the number of spines per pollen. The number of spines per pollen was negatively correlated with the width and length of colpi in the polar view. Multiple sample analysis of variance (ANOVA) concluded that a high statistically significant difference exists among the means of analyzed traits. The examined qualitative and quantitative palynological traits revealed noticeable variations, thus providing the source for species discrimination in Asteraceous tribes.
Hotspots and photon rings in spherically symmetric space–times
Authors: Kocherlakota Prashant , Rezzolla Luciano , Roy Rittick , Wielgus Maciek 
Published: May 2024

ABSTRACTFuture black hole (BH) imaging observations are expected to resolve finer features corresponding to higher order images of hotspots and of the horizon-scale accretion flow. In spherical space–times, the image order is determined by the number of half-loops executed by the photons that form it. Consecutive-order images arrive approximately after a delay time of ≈π times the BH shadow radius. The fractional diameters, widths, and flux-densities of consecutive-order images are exponentially demagnified by the lensing Lyapunov exponent, a characteristic of the space–time. The appearance of a simple point-sized hotspot when located at fixed spatial locations or in motion on circular orbits is investigated. The exact time delay between the appearance of its zeroth and first-order images agrees with our analytic estimate, which accounts for the observer inclination, with $\lesssim 20~{{\ \rm per\ cent}}$ error for hotspots located about ≲ 5M from a Schwarzschild BH of mass M. Since M87⋆ and Sgr A⋆ host geometrically thick accretion flows, we also explore the variation in the diameters and widths of their first-order images with disc scale-height. Using a simple ‘conical torus’ model, for realistic morphologies, we estimate the first-order image diameter to deviate from that of the shadow by $\lesssim 30~{{\ \rm per\ cent}}$ and its width to be ≲ 1.3M. Finally, the error in recovering the Schwarzschild lensing exponent (π), when using the diameters or the widths of the first and second-order images is estimated to be $\lesssim 20~{{\ \rm per\ cent}}$. It will soon become possible to robustly learn more about the space–time geometry of astrophysical BHs from such measurements.
Alternative localization of HEME OXYGENASE 1 in plant cells regulates cytosolic heme catabolism
Authors: Chen Yingxi , Nishimura Kohji , Tokizawa Mutsutomo , Yamamoto Yoshiharu Y , Oka Yoshito , Matsushita Tomonao , Hanada Kousuke , Shirai Kazumasa , Mano Shoji , Shimizu Takayuki , Masuda Tatsuru 
Published: May 2024

Abstract
Heme, an organometallic tetrapyrrole, is widely engaged in oxygen transport, electron delivery, enzymatic reactions, and signal transduction. In plants, it is also involved in photomorphogenesis and photosynthesis. HEME OXYGENASE 1 (HO1) initiates the first committed step in heme catabolism, and it has generally been thought that this reaction takes place in chloroplasts. Here, we show that HO1 in both Arabidopsis (Arabidopsis thaliana) and rice (Oryza sativa) has 2 transcription start sites (TSSs), producing long (HO1L) and short (HO1S) transcripts. Their products localize to the chloroplast and the cytosol, respectively. During early development or de-etiolation, the HO1L/HO1S ratio gradually increases. Light perception via phytochromes (Phys) and cryptochromes elevates the HO1L/HO1S ratio in the whole seedling through the functions of ELONGATED HYPOCOTYL 5 (HY5) and HY5 HOMOLOG and through the suppression of DE-ETIOLATED 1, CONSTITUTIVE PHOTOMORPHOGENESIS 1, and PHYTOCHROME INTERACTING FACTORs. HO1L introduction complements the HO1-deficient mutant; surprisingly, HO1S expression also restores the short hypocotyl phenotype and high pigment content and helps the mutant recover from the genomes uncoupled (gun) phenotype. This indicates the assembly of functional Phys within these lines. Furthermore, our findings support the hypothesis that a mobile heme signal is involved in retrograde signaling from the chloroplast. Altogether, our work clarifies the molecular mechanism of HO1 TSS regulation and highlights the presence of a cytosolic bypass for heme catabolism in plant cells.
The white lupin trehalase gene LaTRE1 regulates cluster root formation and function under phosphorus deficiency
Authors: Xia Tianyu , Zhu Xiaoqi , Zhan Yujie , Liu Bowen , Zhou Xiangxue , Zhang Qian , Xu Weifeng 
Published: May 2024

Abstract
Under phosphorus (P) deficiency, white lupin (Lupinus albus L.) forms a specialized root structure, called cluster root (CR), to improve soil exploration and nutrient acquisition. Sugar signaling is thought to play a vital role in the development of CR. Trehalose and its associated metabolites are the essential sugar signal molecules that link growth and development to carbon metabolism in plants; however, their roles in the control of CR are still unclear. Here, we investigated the function of the trehalose metabolism pathway by pharmacological and genetic manipulation of the activity of trehalase in white lupin, the only enzyme that degrades trehalose into glucose. Under P deficiency, validamycin A treatment, which inhibits trehalase, led to the accumulation of trehalose and promoted the formation of CR with enhanced organic acid production, whereas overexpression of the white lupin TREHALASE1 (LaTRE1) led to decreased trehalose levels, lateral rootlet density, and organic acid production. Transcriptomic and virus-induced gene silencing results revealed that LaTRE1 negatively regulates the formation of CRs, at least partially, by the suppression of LaLBD16, whose putative ortholog in Arabidopsis (Arabidopsis thaliana) acts downstream of ARF7- and ARF19-dependent auxin signaling in lateral root formation. Overall, our findings provide an association between the trehalose metabolism gene LaTRE1 and CR formation and function with respect to organic acid production in white lupin under P deficiency.
Heterogeneous distribution of mitochondria and succinate dehydrogenase activity in human airway smooth muscle cells
Authors: Mahadev Bhat Sanjana , Sieck Gary C. 
Published: May 2024

AbstractSuccinate dehydrogenase (SDH) is a key mitochondrial enzyme involved in the tricarboxylic acid cycle, where it facilitates the oxidation of succinate to fumarate, and is coupled to the reduction of ubiquinone in the electron transport chain as Complex II. Previously, we developed a confocal‐based quantitative histochemical technique to determine the maximum velocity of the SDH reaction (SDHmax) in single cells and observed that SDHmax corresponds with mitochondrial volume density. In addition, mitochondrial volume and motility varied within different compartments of human airway smooth muscle (hASM) cells. Therefore, we hypothesize that the SDH activity varies relative to the intracellular mitochondrial volume within hASM cells. Using 3D confocal imaging of labeled mitochondria and a concentric shell method for analysis, we quantified mitochondrial volume density, mitochondrial complexity index, and SDHmax relative to the distance from the nuclear membrane. The mitochondria within individual hASM cells were more filamentous in the immediate perinuclear region and were more fragmented in the distal parts of the cell. Within each shell, SDHmax also corresponded to mitochondrial volume density, where both peaked in the perinuclear region and decreased in more distal parts of the cell. Additionally, when normalized to mitochondrial volume, SDHmax was lower in the perinuclear region when compared to the distal parts of the cell. In summary, our results demonstrate that SDHmax measures differences in SDH activity within different cellular compartments. Importantly, our data indicate that mitochondria within individual cells are morphologically heterogeneous, and their distribution varies substantially within different cellular compartments, with distinct functional properties.
Parasite‐derived microRNA let‐7‐5p detection for metacestodiasis based on rolling circular amplification‐assisted CRISPR/Cas9
Authors: Wang Liqun , Pu Guiting , Liu Tingli , Chen Guoliang , Li Hong , Amuda Tharheer Oluwashola , Cao Shanling , Yan Hongbin , Yin Hong , Fu Baoquan , Luo Xuenong 
Published: May 2024

AbstractMetacestodiasis is an infectious disease caused by the larval stage of cestode parasites. This disease poses a serious health hazard to wildlife, livestock, and humans, and it incurs substantial economic losses by impacting the safety of the livestock industry, the quality of meat production, and public health security. Unfortunately, there is currently no available molecular diagnostic method capable of distinguishing cysticercus‐ and Echinococcus‐derived microRNAs (miRNAs) from other helminthes and hosts in the plasma of metacestode‐infected animals. This study aims to develop a specific, sensitive, and cost‐efficient molecular diagnostic method for cysticercosis and echinococcosis, particularly for early detection. The study developed a rolling circular amplification (RCA)‐assisted CRISPR/Cas9 detection method based on parasite‐derived miRNA let‐7‐5p. Using a series of dilutions of the let‐7 standard, the limit of detection (LOD) of the qPCR, RCA, and RCA‐assisted CRISPR/Cas9 methods was compared. The specificity of qPCR and CRISPR/Cas9 was evaluated using four artificially synthesized let‐7 standards from different species. A total of 151 plasma samples were used to evaluate the diagnostic performance. Additionally, the study also assessed the correlation between plasma levels of let‐7‐5p, the number of Taenia pisiformis cysticerci, and the weight of Echinococcus multilocularis cysts. The results demonstrated that the RCA‐assisted CRISPR/Cas9 assay could significantly distinguish let‐7 from cestodes and other species, achieving a LOD of 10 aM; the diagnostic sensitivity and specificity for rabbit cysticercosis and mouse E. multilocularis were 100% and 97.67%, and 100% and 100%, respectively. Notably, let‐7‐5p gradually increased in the plasma of T. pisiformis‐infected rabbits from 15 days post infection (dpi), peaked at 60 dpi, and persisted until 120 dpi. In E. multilocularis‐infected mice, let‐7‐5p gradually increased from 15 dpi and persisted until 90 dpi. Furthermore, the expression of let‐7‐5p positively correlated with the number of cysticerci and cyst weight. These results indicated that the let‐7‐5p‐based RCA‐assisted CRISPR/Cas9 assay is a sensitive and specific detection method that can be used as a universal diagnostic method for metacestodiasis, particularly for early diagnosis (15 dpi).