bluuebunny/crossref_metadata_2025_split huggingface dataset:
Arsenic removal by layered double hydroxides (LDH): A mini review
Authors: Turk Tugba , Boyraz Taha , Alp İbrahim 
Published: May 2024

ABSTRACT
The World Health Organization (WHO) has classified arsenic as the most toxic chemical with carcinogenic effects and WHO has established a value of 10 μg/L arsenic as the maximum contaminant level for arsenic in potable water. High selectivity and high removal capacity are one of the most important factors when choosing an adsorbent. Hydrotalcite and hydrotalcite-like compounds have received much attention for the remediation of As-contaminated waters. The most interesting properties of layered double hydroxides (LDHs) include a large surface area and high anion exchange capacity. In this study, the importance of double-layered hydroxides, which has gained importance with the increasing environmental pollution recently, in removing oxyanion pollutants from water has been emphasized. This review article focuses on LDH synthesis and characterization techniques and arsenic removal potential using LDHs, as well as the safe consumability of water obtained after removal. In this review, various factors such as pH, dosage, and temperature that affect oxyanion adsorption on LDHs are discussed. The reusability of LDHs has been discussed. In addition, it has been investigated whether double-layer hydroxides (FeHT) could be used successfully in the removal of arsenic from polluted waters. It was also discussed whether the adsorbent used was safe for health.
The utility of electrode-assisted ammonia oxidation under strict anaerobic conditions
Authors: Kimura Zen-ichiro , Hirata Erika 
Published: May 2024

ABSTRACT

In this study, we investigated the feasibility of electrode-assisted ammonia oxidation and subsequent nitrite accumulation within an anaerobic microbial fuel cell (MFC). Our study focused on determining whether these processes could be effectively maintained in a strictly anaerobic environment, which would be critical for advancing nitrogen removal technologies in wastewater treatment. Although we demonstrated electrode-assisted ammonia oxidation and nitrite accumulation, the expected dominance of these reactions within the system was not achieved. In contrast, ammonia assimilation appeared to be the prevailing nitrogen removal pathway under our experimental conditions. The study did not identify the specific microbial communities responsible for these processes, however, illustrates an important area for future research. Our findings enhance the current understanding of nitrogen cycling in anaerobic MFCs and demonstrate the need for improved strategies to enrich nitrifying bacteria and optimize electrode-assisted nitrogen removal processes in wastewater treatment.
The Regulation of Selenoproteins in Diabetes: A New Way to Treat Diabetes
Authors: Liang Jing , He Yiwen , Huang Chunxia , Ji Fengjie , Zhou Xihong , Yin Yulong 
Published: May 2024

Abstract:
Selenium is an essential micronutrient required for the synthesis and function of selenoproteins,
most of which are enzymes involved in maintaining oxidative balance in the body. Diabetes is a group of
metabolic disorders characterized by high blood glucose levels over a prolonged period of time. There are
three main types of diabetes: type 1, type 2, and gestational diabetes. This review summarizes recent advances
in the field of diabetes research with an emphasis on the roles of selenoproteins on metabolic disturbance in diabetes.
We also discuss the interaction between selenoproteins and glucose and lipid metabolism to provide
new insights into the prevention and treatment of diabetes.
Photochemical Reactions of Sulfur Ylides
Authors: Echemendía Radell , Capellaro Kauê C. , Burtoloso Antonio C.B. 
Published: May 2024

Abstract:
Photochemical reactions offer unparalleled opportunities to access elusive chemical
pathways and develop innovative strategies for constructing complex molecules. Within
organic synthesis, photochemical reactions have become indispensable tools for accessing
complex molecular structures, such as pharmaceuticals and natural products. The ability of
sulfur ylides to participate in these diverse processes has made them indispensable tools in
the synthetic chemist's toolbox. The use of sulfur ylides in photochemical transformations
has garnered significant attention in the synthetic organic chemistry community, and they
serve as powerful intermediates in several chemical transformations. This review article
presents a comprehensive overview of the photochemical reactions mediated by sulfur
ylides. Herein, we describe the key aspects of the reactivity of sulfur ylides in the presence
of light. The reactivity of these compounds can be classified into three categories: sulfur
ylides as energy acceptors, as electron donors, and as trapping reagents.
Protective Effect of Prunetin on Isoproterenol-Induced Myocardial Infarction
in Wistar Rats via Biochemical Characterization
Authors: Liu Rui , Hussain ShaikAlthaf , Maddu Narendra , Wang Xuening 
Published: May 2024

Background:
The development of MI following ischemia damage is influenced by
oxidative stress. Myocardial Infarction (MI) generates myocardial ischemia injury, which damages the cardiomyocytes. Ischemia builds up to a critical level over time in MI, causing permanent myocardial cell damage or death.

Aim:
The current study sought to determine whether Prunetin (PRU) could protect against Isoproterenol (ISO)-induced cardiac heart failure in rats by examining cardiac diagnostic markers,
lipid peroxidation products, enzymatic and non-enzymatic antioxidant levels, and histological
changes.

Methods:
PRU (20 mg/kg bwt) was orally administered for 19 days to rats, and after the treatment, ISO (85 mg/kg bwt) was subcutaneously administered with an intermission of 24 h for a
couple of days to induce myocardial infarction on 20th and 21st days. ISO-treated rats exhibited
considerable alterations in cardiac-sensitive markers in the serum. The levels of lipid peroxidation markers augmented drastically in the plasma and myocardium. Enzymatic antioxidant levels
in erythrocytes and myocardium and the states of non-enzymatic antioxidants were diminished in
the plasma and heart tissue of ISO-treated rats. The histopathological examination of heart tissue
exhibited cardiac damage in ISO-induced rats.

Results:
The oral administration of PRU significantly lowered the levels of lipid peroxidation
and biochemical indicators, while significantly improving the antioxidant system function of
ISO-interposed rats. In PRU-treated ISO-injected rats, histological examinations revealed suppressed myocardial destruction.

Conclusion:
Our research shows that oral pretreatment of PRU prevented ISO-induced oxidative
stress in MI.
Nanotechnology Integrated Innovative Drug Delivery and Therapy for
Cancer
Authors: Macadangdang Romulo R. , Agrawal Rutvi , Bhushan Bharat , Garg Akash , Singh Kuldeep , Kumar Shivendra , Kumar Atul 
Published: May 2024

Abstract:
Cancer remains a significant global health challenge, necessitating innovative approaches to enhance the efficacy and specificity of therapeutic interventions while minimizing
adverse effects on healthy tissues. Nanotechnology has emerged as a promising avenue in cancer
treatment, offering novel strategies for targeted drug delivery. Nanoparticles, liposomes, and
polymer-based systems have played pivotal roles in revolutionizing cancer therapy. Nanotechnology possesses unique physicochemical properties, enabling efficient encapsulation of therapeutic agents and controlled and prolonged release at tumour sites. Advancement in formulations using nanotechnology has made it possible to make multifunctional systems that respond
to the microenvironment of a tumour by releasing payloads in response to changes in pH, temperature, or enzymes. Stimuli-responsive polymers can release drugs in response to external
cues, enabling site-specific drug release and minimizing systemic exposure. This review explores recent studies and preclinical trials that show how nanoparticles, liposomes, and polymerbased systems could be used to treat cancer, discussing challenges such as scalability, regulatory
approval, and potential toxicity concerns along with patents published recently
Burden of Ischemic Heart Diseases among US States from 1990-2019
Authors: Abughazaleh Saeed , Obeidat Omar , Tarawneh Mohammad , Al-Ani Hashim , Nawaiseh Ahmad Al , Ismail Mohamed F. 
Published: May 2024

Background:
Ischemic Heart Disease (IHD) is a leading cause of global mortality, including
in the United States. Understanding the burden of IHD in the United States is crucial for
informed decision-making and targeted interventions aimed at reducing morbidity and mortality
associated with this leading cause of death. This study aimed to understand the burden of IHD,
identify gender disparities and risk factors, explore the relationship between socioeconomic
growth and IHD, and analyze risk factor distribution across the states of the United States.

Methods:
This study utilized data from the Global Burden of Diseases Study 2019, which provided
comprehensive information on IHD from 1990 to 2019. Data related to IHD from these years
were extracted using a query tool from the Institute for Health Metrics and Evaluation (IHME)
website. The study assessed the relationship between IHD and socioeconomic development using
the Socio-demographic Index (SDI) and measured the overall impact of IHD using Disability-adjusted
Life Years (DALYs), considering premature death and disability. Additionally, the study analyzed
the burden of IHD attributed to six main risk factors. Data analysis involved comparing prevalence,
mortality, SDI, DALYs, attributable burden, and risk estimation among the states.

Results:
Between 1990 and 2019, there was an improvement in socioeconomic development in all
states. Age-standardized rates of disease burden for IHD decreased by 50% [ASDR 3278.3 to
1629.4 (95% UI: 1539.9-1712.3) per 100,000] with the most significant decline observed in Minnesota.
Males had higher burden rates than females in all states, and the southeast region had the
highest mortality rates. The prevalence of IHD showed a declining trend, with approximately 8.9
million cases (95% UI: 8.0 million to 9.8 million) in 2019, representing a 37.1% decrease in the
Age-standardized Prevalence Rate (ASPR) from 1990. Metabolic risks were the leading contributors
to the disease burden, accounting for 50% of cases, with Mississippi having the highest attributable
risk. Arkansas had the highest attributable risk for high cholesterol and smoking. Conversely,
Minnesota had the lowest burden of IHD among all the states.

Conclusion:
This study highlights variations in the burden of IHD across US states and emphasizes
the need for tailored prevention programs to address specific risk factors and gender differences.
Understanding the trend in IHD may inform policymakers and healthcare professionals in
effectively allocating resources to reduce the burden of IHD and improve national health outcomes.
Development of Attenuated Total Reflectance Fourier Transform Infrared
Spectroscopy Coupled with Multivariate Classification Chemometric
Model for Routine Screening of Paracetamol, Ibuprofen, and Aspirin
Adulteration in Herbal Products
Authors: Theodore Mario , Vongsutilers Vorasit 
Published: May 2024

Objective:
The objective of this study is to develop and validate a routine screening test
for the determination of three common antipyretic-analgesic synthetic drugs (paracetamol, ibuprofen,
and aspirin) adulteration in herbal products using Attenuated Total Reflectance Fourier Transform
Infrared (ATR-FTIR) coupled with chemometric method.

Methods:
ATR-FTIR spectra of sixteen testing sets of herbal product samples for pain and fever indications
were used for multivariate chemometrics model construction. Linear Discriminant Analysis
(LDA) was selected as a method for model construction with IBM SPSS for statistical analysis.
Model development employed feature selection, such as the stepwise method for variable selection.
The model with a high %correct classification and cross-validation was selected and was
then validated with an independent testing data set with an auto-prediction test, confusion matrix,
and Receiver Operating Characteristic (ROC) curve. To validate the developed test for routine
use, the result from ATR-FTIR method was compared with the standard HPLC and TLC analyses
used for adulteration screening.

Results:
The selected model's overall %correct classification result was 97.7%, with a cross-validation
of 93.8% rate in training set samples. External validation with an independent testing dataset
gave an overall correct classification of 93.8%, with an area under the curve of ROC at
0.979. Comparative testing revealed that model performance was comparable with the HPLC and
TLC methods, which routinely detect the presence of paracetamol, aspirin, and ibuprofen. The results
of testing set samples classification were consistent with training set samples.

Conclusion:
Against the standard chromatographic methods, the multivariate chemometric model
based on ATR-FTIR demonstrates comparable detection capability to determine adulteration of
paracetamol, ibuprofen, and aspirin in herbal products.
Application and Development of Targeted Fishing Technology in Natural
Product Screening - A Simple Minireview
Authors: Su Yingying , Wang Weiping , Wang Ying , Wang Chen , Sun Shuai , Zhu Xianhong , Dai Xiao , Li Shiyu , Gao Xun , Qin Kunming 
Published: May 2024

Background:
The screening of active ingredients in traditional Chinese medicine is an
important task in the modernization of traditional Chinese medicine, and the commonly used analytical
means in the past were mainly to screen the extracts of traditional Chinese medicine
through pharmacological experiments, but the method has major defects. The target fishing strategy
provides a new idea for the screening of active ingredients, and it has rapidly become a hot research
direction, but there are many methods that need to be summarized and aggregated.

Objective:
It aims to provide readers with an understanding of the achievements, developments,
and dilemmas of target fishing techniques over the past few years and to provide new ideas for
subsequent research.

Methods:
Research articles in recent years using target fishing as an entry point are used as a basis
to summarize the types of literature based on their principles and characteristics and to discuss the
advantages and disadvantages of each method.

Conclusion:
This paper summarizes the classification and development of fishing techniques such
as ultrafiltration, equilibrium dialysis, cell membrane chromatography, and immobilization of target
molecules and target fishing and describes the principles and characteristics of these methods.
The applications of these methods in the active ingredients of traditional Chinese medicine are
summarized, and the problems and solutions of these methods are discussed.
The Emerging Role of ADAM 12 Regulates Epithelial-mesenchymal Transition by Activating the Wnt/β-catenin Signaling Pathway in Colorectal Cancer
Authors: Huang Chengchen , Wang Jian , Xiang Jianqin , Wu Chunrong , Wang Fan , Chen Jiangyan , Sun Guiyin , Xiang Debing 
Published: May 2024

Objective:
The objective of this study is to investigate the expression and regulatory
mechanisms of A disintegrin and metalloproteinase domain 12 (ADAM12) in colorectal cancer
(CRC) tissues and cells.

Methods:
Download and analyze the expression levels of ADAM12 in the TCGA and GSE68468
datasets. Collect paraffin-preserved specimens from the Chongqing University Jiangjin Hospital
from April 2017 to December 2019 and detect the expression of ADAM12 through immunohistochemistry.
Cell experiments were conducted using colorectal cancer cell lines (SW480, HCT116),
and cells with high expression of ADAM12 were selected for silencing experiments, and cell proliferation
ability using CCK-8, and migration ability of cells in each group using scratch assay and
Transwell invasion assay. The EMT markers (E-cadherin, N-cadherin, Vimentin, Twist) and the
Wnt/β-catenin markers (β-catenin, GSK-3β, p-GSK-3β, C-MYC, MMP-7) were detected using
western blot. We construct a nude mouse CRC tumor model and validate the effect of ADAM12
on EMT and Wnt/β-catenin through immunohistochemistry and Western blot.

Results:
Bioinformatics showed that increased expression of ADAM12 was strongly correlated
with patient prognosis. Immunohistochemistry showed that elevated ADAM12 was associated
with vascular invasion (p < 0.05), neurological invasion (p < 0.01), lymph node metastasis (p <
0.01), and TNM staging (p < 0.001). Experiments on cell function revealed that the ADAM12
overexpression group augmented CRC cells' proliferation and migration. After overexpression of
ADAM12, the expression of N-cadherin, Vimentin, and Twist increased, while the expression of
E-cadherin decreased (p < 0.01). The expression of Proteins related to Wnt/β-catenin: β-catenin,
p-GSK-3 β, C-MYC and MMP-7 increased (p < 0.01), and Wnt/β-catenin inhibitor MSAB can
counteract the effect of ADAM12 on EMT in CRC cells. The subcutaneous tumor formation experiment
results in nude mice showed that ADAM12 promoted tumor growth and induced EMT
compared to the control group.

Conclusion:
ADAM12 overexpression through the Wnt/β-catenin signal axis controls the EMT of
CRC to promote invasion and metastasis.
CRISPR/Cas9 Gene Editing: A Novel Approach Towards Alzheimer's Disease Treatment
Authors: Tripathi Siddhant , Sharma Yashika , Rane Rajesh , Kumar Dileep 
Published: May 2024

:
In defiance of the vast amount of information regarding Alzheimer's disease (AD) that
has been learned over the past thirty years, progress toward developing an effective therapy has
been difficult. A neurological ailment that progresses and cannot be reversed is Alzheimer's disease,
which shows neurofibrillary tangles, beta-amyloid plaque, and a lack of cognitive processes that is
created by tau protein clumps with hyperphosphorylation that finally advances to neuronal damage
without a recognized treatment, which has stimulated research into new therapeutic strategies. The
protein CAS9 is linked to CRISPR, which is a clustered Regularly Interspaced Short Palindromic
Repeat that inactivates or corrects a gene by recognizing a gene sequence that produces a doublestranded
break has enchanted a whole amount of interest towards its potency to cure gene sequences
in AD. The novel CRISPR-Cas9 applications for developing in vitro and in vivo models to the benefit
of AD investigation and therapies are thoroughly analyzed in this work. The discussion will also
touch on the creation of delivery methods, which is a significant obstacle to the therapeutic use of
CRISPR/Cas9 technology. By concentrating on specific genes, such as those that are significant early-
onset AD risk factors and late-onset AD risk factors, like the apolipoprotein E4 (APOE4) gene,
this study aims to evaluate the potential application of CRISPR/Cas9 as a possible treatment for AD.
Guideline Adherence as an Indicator of PET Scan Overuse in an Italian Teaching Hospital: An Observational Study
Authors: Cascini Giuseppe Lucio , Mauro Marianna , Catalfamo Barbara , Giancotti Monica , Muraca Roberta 
Published: May 2024

Background:
Evidence of inappropriate overuse and underuse of medical procedures
has been documented in modern healthcare systems around the world. Excessive use of health
services can contribute to a rapid increase in healthcare costs and harm the patient physically
and psychologically; conversely, underuse can lead to the inability to provide effective treatments
when clinically indicated.

Objective:
The study's aim is twofold: a) to measure the appropriateness of PET prescription in
a cohort of patients, offering empirical evidence of overuse of health care services; b) to evaluate
how the overuse of PET could affect public health expenditure and, consequently, the system's
financial sustainability.

Methods:
In this observational study, we have analyzed prospectively and retrospectively health
patient records who underwent 18F-FDG PET/TC scan at the Nuclear Medicine Department of
the University Hospital Mater Domini in Catanzaro (Italy) from 29/09/2022 to 10/02/2023. Patients’
diagnostic questions have been defined as appropriate, not completely appropriate and
completely inappropriate according to the 18F-FDG PET/CT recommendations defined by the
“Conditions of Supply and Indications of Prescriptive Appropriateness of Italian NHS (National
Health Systems)” published in the Official Gazette no. 15 of 20 January 2016 (Decree 9 December
2015) and by the AIMN (Italian Association of Nuclear Medicine) guidelines.

Results:
We gathered data from 500 oncological patients (242 males and 258 females). The results
show that 423/500 of patients' prescriptions were appropriate, while 77/500 of patients'
prescriptions were completely inappropriate (63/77) or not completely appropriate (14/77).

Conclusion:
Analysis showed a not complete adherence to national guidelines and no shared
decision-making approach.
Investigation of Unsaturated Soil Hydraulic Properties for Subgrade Improvement using Marble Dust Waste
Authors: Hafiz Harith , Kechik Fakhrurazi Awang , Ibrahim Aniza , Taib Aizat Mohd , Hasbollah Dayang Zulaikha Abang , Dan@Azlan Mohd. Firdaus Md 
Published: May 2024

Introduction
The distribution of suction, which has implications for seepage and shear strength, plays a crucial role in determining the stability of unsaturated soil. The Air Entry Value (AEV) and Residual Water Contents (RWC) change over time to create the Soil-Water Characteristic Curve (SWCC), which is very important for figuring out how the suction is distributed. During a flood occurrence, the subgrade becomes inundated with water, leading to adverse effects on the AEV and RWC of the subgrade. Consequently, the performance of the subgrade is diminished. This study focuses on the use of Marble Dust Waste (MDW) as an addition to the subgrade to improve the strength of the subgrade.

Aims
This study aims to investigate the variations in air AEV in relation to the optimal MDW in unsaturated soil found in Malaysia.

Methods
The study uses control samples and soil that has been mixed with different amounts of MDW: 5%, 10%, 15%, 20%, and 25%. The SWCC is generated using the pressure plate extractor device.

Results
The results indicate a significant increase in the AEV for the mixed soil sample when compared to the control sample. While the soil-MDW mixture containing 5% of this material has an AEV value of 23 kPa, the control sample's value is 10 kPa.

Conclusion
The findings of the study suggest that the utilization of MDW yields a beneficial influence on the AEV, hence potentially improving the performance of the subgrade. The researchers aim to evaluate the potentially dangerous waste and convert it into a substance that is appropriate for engineering applications. This study provides empirical evidence that aligns with the objectives outlined in Chapter 8 of the 12th Malaysia Plan for the period 2021–2025. The topic under discussion pertains to environmental sustainability, specifically focusing on enhancing the ability to withstand the adverse effects of climate change and disasters, as well as the Green Technology Master Plan Malaysia 2017–2030, with a specific emphasis on Chapter 6, which addresses the issue of waste management.