bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human osteoblast behavior on as‐synthesized SiO4 and B‐CO3 co‐substituted apatite
Authors: Landi Elena , Uggeri Jacopo , Sprio Simone , Tampieri Anna , Guizzardi Stefano 
Published: January 2010

AbstractThe functional behavior of synthetic apatite, commonly used as fillers or scaffolds, depends on physical and chemical parameters, which vary in response to chemical substitutions and to thermal treatments. The effect of silicon co‐substituting with carbonate ions in the apatite lattice on the properties of the as‐synthesized powder and finally on human osteoblast in vitro behavior was investigated. Dose‐response curves of Si‐free and Si‐substituted carbonated apatites (namely CHA and SiCHA‐1 and SiCHA‐2 with 0.88 and 0.55 wt % of Si, respectively) showed that SiCHA‐1 had toxic effect, whereas CHA and SiCHA‐2, at worst, hindered osteoblast proliferation, but no toxicity occurred. Subsequent experiments compared the effects of CHA and SiCHA‐2 used at the doses of 0.3 and 1 mg/mL. After 7 days of treatment, both the powders stimulated cell proliferation and protein content and inhibited alkaline phosphatase activity. However, SiCHA‐2 slightly stimulated osteoblast differentiation, as shown by higher calcium deposition, compared with CHA. The cell behaviors were linked to the peculiar powder characteristics. The as‐synthesized powder represents the most critical system in terms of reactivity toward cells and can inform on the limits for positively exploiting the characteristics of SiCHA powders in making bone fillers or scaffolds, using no thermal treatments. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Plasma mediated collagen‐I‐coating of metal implant materials to improve biocompatibility
Authors: Hauser Joerg , Koeller Manfred , Bensch Sebastian , Halfmann Helmut , Awakowicz Peter , Steinau Hans‐Ulrich , Esenwein Stefan 
Published: January 2010

AbstractThis study describes the collagen‐I coating of titanium and steel implants via cold low‐pressure gas plasma treatment. To analyze the coatings in terms of biocompatibility osteoblast‐like osteosarcoma cells and human leukocytes were cultivated on the metal surfaces. Two different implant materials were assessed (Ti6Al4V, X2CrNiMo18) and four different surface properties were evaluated: (a) plasma pretreated and collagen‐I coated implant materials; (b) collagen‐I dip‐coated without plasma pretreatment; (c) plasma treated but not collagen‐I coated; (d) standard implant materials served as control. The different coating characteristics were analyzed by scanning electron microscopy (SEM). For adhesion and viability tests calcein‐AM staining of the cells and Alamar blue assays were performed. The quantitative analysis was conducted by computer assisted microfluorophotography and spectrometer measurements. SEM analysis revealed that stable collagen‐I coatings could not be achieved on the dip‐coated steel and titanium alloys. Only due to pretreatment with low‐pressure gas plasma a robust deposition of collagen I on the surface could be achieved. The cell viability and cell attachment rate on the plasma pretreated, collagen coated surfaces was significantly (p < 0.017) increased compared to the non coated surfaces. Gas plasma treatment is a feasible method for the deposition of proteins on metal implant materials resulting in an improved biocompatibility in vitro. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res, 2010
Addition of nimesulide to small intestinal submucosa biomaterial inhibits postsurgical adhesiogenesis in rats
Authors: Suckow Mark A. , Hodde Jason P. , Wolter William R. , Wood Karl V. , Hiles Michael C. , Janis Abram D. 
Published: January 2010

AbstractAdhesion formation is a common complication in abdominal surgery with incidence as high as 93% and small bowel obstruction a common complication. Because the extracellular matrix material, small intestinal submucosa (SIS), is commonly used in various surgical procedures, methods to inhibit adhesiogenesis are of great interest. This study was undertaken to determine if incorporation of nimesulide (NM), a selective cyclooxygenase (COX)‐2 inhibitor, could reduce the extent and tenacity of intraabdominal adhesion formation associated with SIS implantation. Female Sprague–Dawley rats underwent a cecal abrasion surgical procedure to induce adhesiogenesis. Rats were either left untreated or treated by direct application over the injured cecum with polypropylene mesh (PPM); SIS; SIS containing a low dose of NM; or SIS containing a high dose of NM. Rats were euthanized 21 days later, and adhesion extent and tenacity were evaluated using standard scales (0 = minimal adhesiogenesis; 4 = severe adhesiogenesis). Addition of NM to SIS resulted in a significant (p < 0.05) reduction in adhesion extent and in a similar reduction in adhesion tenacity for SIS containing a low dose of NM. Adhesions typically extended from the abraded cecal surface to the body wall and were characterized histologically by fibrous tissue adherent to the cecal wall. In conclusion, addition of the nonsteroidal anti‐inflammatory, COX‐2 selective drug, NM, to SIS attenuates adhesion extent and tenacity when compared with surgical placement of SIS or PPM alone. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
The effect of static applied potential on the 24‐hour impedance behavior of commercially pure titanium in simulated biological conditions
Authors: Ehrensberger Mark T. , Gilbert Jeremy L. 
Published: January 2010

AbstractPotential step impedance analysis was utilized to evaluate the electrochemical impedance of commercially pure titanium (cpTi) samples that were polarized to static potentials (range from −1000 mV to +1000 mV vs. Ag/AgCl) and immersed in physiologically relevant electrolytes [phosphate buffered saline (PBS) and cell culture medium with 10% fetal bovine serum (AMEM + FBS)] for 24 hrs. The cpTi impedance outcomes were a complex function of voltage, solution constituents, and immersion time. In the 0 mV to +1000 mV range, oxide growth was observed over 24 hr immersion in both solutions based on decreasing current density (∼10−6 A/cm2 to ∼10−8 A/cm2) and increasing Rp (200 kΩ cm2 to ∼10 MΩ cm2). Below 0 mV, the 24 hr Rp decreased with negative potential to ∼15 kΩ cm2. After 24 hr immersion, oxide dissolution and/or adsorption of organic species caused the capacitance to increase at −1000 mV (AMEM + FBS & PBS) and at −600 mV (AMEM + FBS only). Twenty‐four hours of immersion in AMEM + FBS at −1000 mV and −600 mV produced a surface coloration that is likely due to alteration of oxide valance state and/or doping level. This work shows that Ti surface oxide and its electrochemical behavior can be altered dramatically under sustained cathodic potentials. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Effect of curing mode on bond strength of self‐adhesive resin luting cements to dentin
Authors: Aguiar T. R. , Di Francescantonio M. , Ambrosano G. M. B. , Giannini M. 
Published: January 2010

AbstractIn this study, the in vitro bond strength of dual‐curing resin cements to indirect composite restorations when the cement was either light polymerized or allowed to only autopolymerize was evaluated. Occlusal dentin surfaces of 56 extracted human third molars were flattened to expose coronal dentin. Teeth were assigned to eight groups (n = 7) according to resin cement products and polymerization modes: conventional cement (Panavia F 2.0; Kuraray Medical) and self‐adhesive cements [RelyX Unicem (3M ESPE), BisCem (Bisco), and G‐Cem (GC Corp.)]. Cements were applied to prepolymerized resin discs (2‐mm‐thick Sinfony; 3M ESPE), which were subsequently bonded to the prepared dentin surfaces. The restored teeth were either light‐polymerized through the overlying composite according to manufacturers' instructions or were allowed to only self‐cure. After 24 h, the teeth and restorations were sectioned to obtain multiple bonded beams (1.0 mm2) and tested in tension at a crosshead speed of 0.5 mm/min until failure. Data (MPa) were analyzed by two‐way ANOVA and Tukey test (α = 0.05). Light activation of some cement systems (G‐Cem and Panavia F 2.0) increased the bond strength, while the curing mode did not affect the bond strength for some (RelyX Unicem and BisCem). The bond strength in the autopolymerized mode varied among products. In general, the use of self‐adhesive resin cements did not provide significantly higher bond strengths than that of a conventional material, and two self‐adhesive cements yielded significantly lower bond values (regardless of cure mode) than the other products. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Mechanical, permeability, and degradation properties of 3D designed poly(1,8 octanediol‐co‐citrate) scaffolds for soft tissue engineering
Authors: Jeong Claire G. , Hollister Scott J. 
Published: January 2010

AbstractPoly(1,8‐octanediol‐co‐citric acid) (POC) is a synthetic biodegradable elastomer that can be processed into three‐dimensional (3D) scaffolds for tissue engineering. We investigated the effect of designed porosity on the mechanical properties, permeability, and degradation profiles of the POC scaffolds. For mechanical properties, scaffold compressive data were fitted to a one‐dimensional (1D) nonlinear elastic model, and solid tensile data were fitted to a Neohookean incompressible nonlinear elastic model. Chondrocytes were seeded on scaffolds to assess the biocompatibility of POC. Increased porosity was associated with increased degradation rate, increased permeability, and decreased mechanical stiffness, which also became less nonlinear. Scaffold characterization in this article will provide design guidance for POC scaffolds to meet the mechanical and biological parameters needed for engineering soft tissues such as cartilage. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Effect of artificial aging regimens on the performance of self‐etching adhesives
Authors: Ülker Mustafa , Özcan Mutlu , Şengün Abdulkadir , Özer Füsun , Belli Sema 
Published: January 2010

AbstractThis in vitro study was performed to compare the microtensile bond strengths (MTBS) of current self‐etching adhesives to dentin and to evaluate the effects of artificial aging [(thermocycling (TC) and/or mechanic loading (ML)] on MTBS and on nanoleakage of self‐etching adhesives. Two‐step (AdheSE Bond, Clearfil Protect Bond, Clearfil SE Bond, Optibond Self‐Etch) and one‐step (Hybrid Bond, G‐bond, Clearfil Tri‐S Bond, and Adper Prompt L‐Pop) self‐etching adhesives were tested. Resin composite build‐ups were created, and the specimens were subjected to 104 TC, 105 ML, and 104/105 TC/ML. Non‐aged specimens served as controls. In the control group, no significant differences were found among the MTBS of the one‐step self‐etching adhesives and among those of three two‐step self‐etching adhesives (AdheSE Bond, Clearfil Protect Bond, and Clearfil SE Bond) (p > 0.05). The MTBS of AdheSE Bond and Clearfil Protect Bond were higher than were those of all one‐step self‐etching adhesives and than those of Optibond Self‐Etch. The MTBS of Clearfil SE Bond was higher than were those of two one‐step self‐etching adhesives (Adper Prompt L‐Pop, G‐bond) (p < 0.05). Compared with the non‐aged controls, TC did not decrease (p > 0.05), but ML and TC/ML significantly decreased the MTBS of the adhesives tested (p < 0.05). Two‐step self‐etching adhesives tended to fail more cohesively in dentin. Transmission electron microscopy revealed different nanoleakage patterns in the adhesive and hybrid layers of all adhesives examined, and signs of additional silver‐filled water channels were more readily detectable after TC/ML. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Ceramic scaffolds produced by computer‐assisted 3D printing and sintering: Characterization and biocompatibility investigations
Authors: Warnke Patrick H. , Seitz Hermann , Warnke Frauke , Becker Stephan T. , Sivananthan Sureshan , Sherry Eugene , Liu Qin , Wiltfang Jörg , Douglas Timothy 
Published: January 2010

AbstractHydroxyapatite (HAP) and tricalcium phosphate (TCP) are two very common ceramic materials for bone replacement. However, in general HAP and TCP scaffolds are not tailored to the exact dimensions of the defect site and are mainly used as granules or beads. Some scaffolds are available as ordinary blocks, but cannot be customized for individual perfect fit. Using computer‐assisted 3D printing, an emerging rapid prototyping technique, individual three‐dimensional ceramic scaffolds can be built up from TCP or HAP powder layer by layer with subsequent sintering. These scaffolds have precise dimensions and highly defined and regular internal characteristics such as pore size. External shape and internal characteristics such as pore size can be fabricated using Computer Assisted Design (CAD) based on individual patient data. Thus, these scaffolds could be designed as perfect fit replacements to reconstruct the patient's skeleton. Before their use as bone replacement materials in vivo, in vitro testing of these scaffolds is necessary. In this study, the behavior of human osteoblasts on HAP and TCP scaffolds was investigated. The commonly used bone replacement material BioOss® served as control. Biocompatibility was assessed by scanning electron microscopy (SEM), fluorescence microscopy after staining for cell vitality with fluorescin diacetate (FDA) and propidium iodide (PI) and the MTT, LDH, and WST biocompatibility tests. Both versions were colonised by human osteoblasts, however more cells were seen on HAP scaffolds than TCP scaffolds. Cell vitality staining and MTT, LDH, and WST tests showed superior biocompatibility of HAP scaffolds to BioOss®, while BioOss® was more compatible than TCP. Further experiments are necessary to determine biocompatibility in vivo. Future modifications of 3D printed scaffolds offer advantageous features for Tissue Engineering. The integration of channels could allow for vascular and nerve ingrowth into the scaffold. Also the complex shapes of convex and concave articulating joint surfaces maybe realized with these rapid prototyping techniques. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Hyperosmolarity enhanced susceptibility to renal tubular fibrosis by modulating catabolism of type I transforming growth factor‐β receptors
Authors: Chiang Tai‐An , Yang Yu‐Lin , Yang Ya‐Ying , Hu Min‐Hsiu , Wu Pei‐Fen , Liu Shu‐Fen , Huang Ruay‐Ming , Liao Tung‐Nan , Hung Chien‐Ya , Hung Tsung‐Jen , Lee Tao‐Chen 
Published: January 2010

AbstractHyperosmolarity plays an essential role in the pathogenesis of diabetic tubular fibrosis. However, the mechanism of the involvement of hyperosmolarity remains unclear. In this study, mannitol was used to evaluate the effects of hyperosmolarity on a renal distal tubule cell line (MDCK). We investigated transforming growth factor‐β receptors and their downstream fibrogenic signal proteins. We show that hyperosmolarity significantly enhances the susceptibility to exogenous transforming growth factor (TGF)‐β1, as mannitol (27.5 mM) significantly enhanced the TGF‐β1‐induced increase in fibronectin levels compared with control experiments (5.5 mM). Specifically, hyperosmolarity induced tyrosine phosphorylation on TGF‐β RII at 336 residues in a time (0–24 h) and dose (5.5–38.5 mM) dependent manner. In addition, hyperosmolarity increased the level of TGF‐β RI in a dose‐ and time‐course dependent manner. These observations may be closely related to decreased catabolism of TGF‐β RI. Hyperosmolarity significantly downregulated the expression of an inhibitory Smad (Smad7), decreased the level of Smurf 1, and reduced ubiquitination of TGF‐β RI. In addition, through the use of cycloheximide and the proteasome inhibitor MG132, we showed that hyperosmolarity significantly increased the half‐life and inhibited the protein level of TGF‐β RI by polyubiquitination and proteasomal degradation. Taken together, our data suggest that hyperosmolarity enhances cellular susceptibility to renal tubular fibrosis by activating the Smad7 pathway and increasing the stability of type I TGF‐β receptors by retarding proteasomal degradation of TGF‐β RI. This study clarifies the mechanism underlying hyperosmotic‐induced renal fibrosis in renal distal tubule cells. J. Cell. Biochem. 109: 663–671, 2010. © 2010 Wiley‐Liss, Inc.
Giant cell tumor of a tendon sheath mimicking an axillary lymph node
Authors: Sohn Yu‐Mee , Kim Ah‐Hyun , Choi Junjeong , Kim Eun‐Kyung , Moon Hee Jung , Kim Min Jung , Park Young Nyun , Kwak Jin Young 
Published: January 2010

AbstractA giant cell tumor of the tendon sheath (GCTTS) is 1 of the most common soft‐tissue tumors of the hand and wrist, while the 2nd most frequent site is the ankle‐foot complex. Although various solid tumors can develop in the axilla, GCTTS has not yet been reported. We describe the sonographic appearance of GCTTS in the axilla. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound 2010
Diagnostic accuracy of real‐time 3D sonography in the diagnosis of congenital Mullerian anomalies in high‐risk patients with respect to the phase of the menstrual cycle
Authors: Caliskan Eray , Ozkan Sabiha , Cakiroglu Yigit , Sarisoy Hasan Tahsin , Corakci Aydin , Ozeren Semih 
Published: January 2010

AbstractPurpose.To compare the diagnostic accuracy of 2‐dimensional sonography (2DUS) and real‐time 3‐dimensional sonography (3DUS) in the diagnosis of congenital mullerian defects (CMD) with respect to the phase of the menstrual endometrium.Method.The accuracy of sonography was examined on 108 women by 2 gynecologists during the 1st 5 days after cessation of menstrual flow and then re‐examined at the cycle days 20–24. Entrance criteria for the patients enrolled in the study were as follows: women who were referred to our center with a suspected Mullerian anomaly at hysterosalpingography, and women who were suspected to have a uterine anomaly at our hospital during infertility, dysmenorrhea, and recurrent abortion workup. First, 1 of the gynecologists performed the 2DUS, and afterwards the 2nd gynecologist performed the real‐time 3DUS. Results from both examiners were compared and correlated with the definitive diagnosis obtained by MRI, laparoscopy, or hysteroscopy. The sensitivity and specificity values of 2DUS and real‐time 3DUS for the diagnosis of CMD were calculated at follicular and luteal phases.Result.Among the 108 cases suspected to have CMD, the sensitivity and specificity values of real‐time 3DUS were significantly higher in the follicular sensitivity, 94.7%, specificity, 75.0%, and luteal phases (sensitivity, 100%, specificity, 93.7%) when compared with 2DUS values (sensitivity of 30.2% and specificity of 78.1% in the follicular phase and sensitivity of 42.1% and specificity of 81.2% in the luteal phase).Conclusion.Real‐time 3DUS is an accurate method that can be used for the diagnosis of congenital mullerian defects. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound 2010
McKusick‐Kaufman syndrome: The difficulty of establishing a prenatal diagnosis of an uncommon disorder
Authors: Slavin Thomas P. , McCandless Shawn E. , Lazebnik Noam 
Published: January 2010

AbstractPrenatal evaluation of abdominal cystic masses can be complex and challenging. We report the case of a fetus with a large cystic abdominal mass and discuss how the differential diagnosis was narrowed to include McKusick‐Kaufman syndrome (MKS). MKS is characterized by the triad of postaxial polydactyly, congenital heart disease, hydrometrocolpos, and genital malformations in males. Rare conditions such as MKS are difficult to diagnose prenatally and require postnatal phenotyping and molecular studies before a definitive diagnosis can be established. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound 2010
Is neonatal risk from vasa previa preventable? The 20‐year experience from a single medical center
Authors: Smorgick Noam , Tovbin Yosef , Ushakov Fred , Vaknin Zvi , Barzilay Bernard , Herman Arie , Maymon Ron 
Published: January 2010

AbstractBackground.Vasa previa is a rare condition associated with neonatal morbidity and mortality that may be diagnosed prenatally using transvaginal sonography. The aim of this study was to assess the prenatal detection of vasa previa and its subsequent impact on neonatal outcomes in two 10‐year periods (1988–1997 versus 1998–2007).Method.Retrospective review of all cases of vasa previa. Data on obstetrical history, modes of conception, sonographic scans, delivery mode, and neonatal outcome were retrieved and recorded.Result.There were 19 pregnancies (21 neonates) with confirmed vasa previa (overall incidence of 1.7/10,000 deliveries). Vasa previa were diagnosed prenatally in 10 (52.6%) cases. In cases without prenatal diagnosis, there was a higher proportion of neonates with 1′ Apgar score ≤5 and cord blood pH <7 compared with cases diagnosed prenatally (66.7% versus 10%, p ≤ 0.05, and 33.3% versus 0%, p < 0.05, respectively). The prenatal detection rate of vasa previa increased from 25 to 60% between the 2 time periods (p > 0.05), whereas perinatal mortality and 1′ Apgar scores ≤5 decreased from 25 to 0% and from 50 to 33.3% (p > 0.05).Conclusion.Prenatal sonographic screening using targeted scans for vasa previa in women at risk or as part of routine mid‐gestation scanning may significantly impact its obstetric manifestations. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound, 2010
On the purity of 2‐[ortho‐anilinyl]‐1,3‐benzoxazole derived from 2H‐3,1‐benzoxazine‐2,4(1H)dione (isatoic anhydride) [1,2]
Authors: Button Karen M. , Gossage Robert A. , Jenkins Hilary A. , Mahdi Tayseer , Resanović Sanja 
Published: January 2010

Abstract
magnified image The Lewis acid catalyzed synthesis and chromatographic purification of isatoic anhydride‐derived 2‐(2′‐anilinyl)‐1,3‐benzoxazole (2) can result in the co‐isolation of 2 and a light pink colored impurity (<5%). This latter species has been identified (NMR, single crystal X‐ray diffraction, mp) as 2′‐hydroxy‐2‐aminobenzanilide (3), which represents a predictable intermediate in the formation of 2. Compound 3 crystallizes in an orthorhombic crystal system of space group P212121 with four molecules in the unit cell (α = β = γ = 90°; a = 6.715 (2) Å, b = 12.100 (4) Å, c = 13.321 (4) Å; V = 1082.2 (6) Å3). Pure 2 is characterized as a colorless, high‐melting solid; unlike the dark colored oil that is isolated if 2 contains traces of 3. J. Heterocyclic Chem., (2010).