bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-throughput sequencing reveals crebanine inhibits colorectal cancer by modulating Tregs immune prognostic target genes
Authors: Xu Jiajun , Huang Lingyu , Sha Yu , Mo Chune , Gong Weiwei , Tian Xiayu , Hou Xianliang , Chen Wei , Ou Minglin 
Published: May 2024

Abstract

Objectives
Crebanine, an alkaloid exhibiting sedative, anti-inflammatory, and anticancer properties, remains unexplored in terms of its anticancer potential against colorectal cancer (CRC). This study aims to bridge this knowledge gap, specifically investigating whether crebanine can suppress CRC and elucidating its underlying molecular mechanism.

Methods
We employed the MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay, cell scratch assay, and flow cytometry to observe the effects of crebanine on the growth, migration, and apoptosis of CRC SW480 cells, respectively. High-throughput sequencing was employed to detect differentially expressed genes (DEGs) in SW480 cells treated with crebanine. Enriched pathways of these DEGs were identified through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis. Genes exhibiting the highest correlation in the enriched pathway were further analyzed using clinical data from The Cancer Genome Atlas Program (TCGA) public database, utilizing R software.

Results
Crebanine effectively inhibited the proliferation, migration, and invasion of SW480 cells, with concentrations of ≥15 μg/mL promoting apoptosis. Analysis revealed that the function of DEGs linked to the most enriched pathways was associated with immune infiltration by regulatory T cells (Tregs). When analyzed in conjunction with clinical data, the genes exhibiting the highest correlation in the enrichment pathway were found to be directly associated with clinical prognostic survival.

Conclusions
Our study demonstrates that crebanine inhibits colorectal cancer by regulating prognostic target genes related to Tregs. This finding offers a novel approach for pharmacological inhibition and Tregs-targeted therapy in CRC.
Effect of multi-walled carbon nanotubes reinforcement on self-healing performance of natural rubber
Authors: Mohd Sani Noor Faezah , Shuib Raa Khimi , Zainol Mohd Hafiz , Othman Nadras , Tran Dai Lam 
Published: May 2024

Abstract
This work is motivated by the desire to restore the quality of rubber-based product properties with the intention of prolonging the service life period, thus helping create a sustainable environment by proposing effective rubber waste management. This study experimentally investigated an intrinsically self-healing zinc thiolate grafted natural rubber (NRZT) compound filled with multi-walled carbon nanotubes (MWCNT) to assess its influence on mechanical properties and self-healing performance. The MWCNT loading varied to 0, 2, 4, 6, and 8 phr. The Equilibrium swelling test was used to quantify the amount of ionic and covalent crosslinks formed. Fourier Transform Infrared (FTIR) spectra were used to detect the presence of MWCNT in the compound. The mechanical properties computed by the tensile and tear strength tests showed that the incorporation of MWCNT increased both properties up to three and twofold, respectively. However, as expected, the elongation at break (Eb) value was reduced. The unfilled sample showed that it can heal up to ∼98 %, measured from the tensile strength. However, the healing efficiency obtained from tensile strength reduces to ∼88 % by incorporating 2 phr MWCNT. The Eb and its self-healing efficiency gradually decreased as the MWCNT amount increased. All samples showed outstanding properties under the tearing mode, where the healed samples produced higher tear strength (>100 % healing) than the initial value. Scanning Electron Microscopy (SEM) micrographs revealed a noticeable gap along the healed cut line with increased MWCNT numbers, possibly due to the lower reaction between polymerized zinc thiolate (PZTh) radicals with zinc thiolate (ZT) and rubber molecules. The work aims to investigate the influence of MWCNTs on the mechanical and healing performance of self-healing NR composites by comparing them to their unfilled sample.
As Camp as a Row of Pink Tents: Stephen’s Portrait of Mr W. S.
Authors: Slote Samuel 
Published: May 2024

In the ‘Scylla and Charybdis’ episode of Ulysses, Stephen Dedalus presents a theory about Shakespeare’s biographical motivations for writingHamlet, which he ultimately claims, perhaps disingenuously, to not believe. Stephen’s apparent disbelief in his own theory echoes Oscar Wilde’s‘Portrait of Mr W. H.’, which is referenced within ‘Scylla’, and which also propounds a theory of Shakespeare’s artistic production in terms of his biography. Furthermore, like the various characters in Wilde’s story, Stephen’s theory is propelled primarily from the internal evidence of Shakespeare’s texts. In this article, I will analyse the playful and learned insincerity of both theories through the optic of camp in order to tease out the implications that Stephen’s argument about Shakespeare has for James Joyce’s aesthetics.
Primary Data on ATTR-Amyloidosis Prevalence Among Elderly Patients With Left Ventricular Hypertrophy in Russia
Authors: Nikiforova T. V. , Charaya K. V. , Shchekochikhin D. Yu. , Magomedova Z. M. , Enokyan M. S. , Volovchenko A. N. , Khamzatkhanova A. H. , Starovoytova T. A. , Bogdanova A. A. , Karalkin A. V. , Pasha S. P. , Pershina E. S. , Grachev A. E. , Zhirov I. V. , Andreev D. A. 
Published: May 2024

Aim. To estimate the prevalence of amyloid cardiomyopathy (CM) caused by transthyretin amyloidosis (ATTR) and immunoglobulin light chain (AL) amyloidosis among patients aged >65 years with interventricular septal (IVS) hypertrophy of ≥14 mm.Material and methods. From January through August 2023, 60 patients (mean age 7.2±7.3 years, 34 (56.67%) men) were enrolled. Patients meeting the inclusion criteria underwent an echocardiographic study with determining the myocardial longitudinal strain, myocardial scintigraphy with 99mTc-pyrfotech, myocardial single-photon emission computed tomography, measurement of N-terminal fragment of brain natriuretic peptide and troponin I, and the immunochemical study of serum and urine proteins with measurement of free light chains. In the presence of grades 2 and 3 radiopharmaceutical uptake according to scintigraphy, a molecular genetic study was performed for differential diagnosis of wild-type transthyretin amyloidosis (wtATTR) and hereditary/variant (hATTR) ATTR-CM.Results. According to data of myocardial scintigraphy with 99mTc-pyrfotech, grade 3 uptake in the absence of monoclonal secretion was detected in 5 (8.3%) cases and grade 2 radiotracer uptake in the absence of monoclonal secretion was detected in 6 (10%) patients. Myeloma complicated by AL amyloidosis and primary AL amyloidosis were found in 5 (8.3%) patients.Conclusion. Among patients aged ≥65 years with IVS hypertrophy ≥14 mm, amyloid CM was detected in 20% of cases (12 patients), including 5 cases (8.3%) of AL amyloidosis and 7 cases (11.7%) of ATTR amyloidosis.
Location of mandibular foramen in dry mandibles in relation to various anatomical landmarks
Authors: Subramanian Manickam , Pavithra M  , Bandlamudi Sowjanya , Mariappan Arathi Senthiappan , Yoganandham Janani 
Published: May 2024

To localize the mandibular foramen in dry mandibles of adult and old age groups of South Indian origin by estimating its average distance from different anatomical landmarks and to provide a specific and precise site for inferior alveolar nerve block. : A total of 51 mandibles were taken for the study and the distance between the mandibular foramen and the various anatomical landmarks were measured using a digital vernier caliper. Presence of accessory mandibular foramen was observed and recorded. Difference between the sides and the correlation between the gonial angle and various distances were carried out.:Distance of mandibular foramen from anterior border was 16.41±2.44 mm and 16.85±2.55 mm, from posterior border was 10.28±2.08 mm and 10.13±2.02 mm, from mandibular incisure 22.5±3.13mm and 21.9±3.08mm, from mandibular base 23.72±3.37 mm and 24.5±3.27 mm, from third molar 15.27±11.99 mm and 16.85±11.60 mm, from apex of retro molar trigone 18.28±3.02 mm and 19.79±3.18 mm on right and left side respectively. There was no significant difference between the measurements of right and left sides. The measurements had a negative association with gonial angle. Accessory foramina were found to be present in 21and 23 mandibles on right and left side respectively.:Precise localization of mandibular foramen is clinically very important to achieve effective inferior alveolar nerve block prior to dental surgeries in the lower jaw. The present study establishes the presence of bilateral symmetry in the position of mandibular foramen with respect to various anatomical landmarks and the utility of gonial angle in localizing the mandibular foramen.
X-ray structural study of membranes based on Na-CMC and CS-Cl for water purification
Authors: Demchenko Valerii , Shtompel Volodymyr , Ovsiankina Victoria , Broshko Oleh , Krysenko Tamara , Yurzhenko Maksym , Romaniukina Iryna 
Published: May 2024

The level of environmental pollution is increasing year by year. Solving this problem requires the development and application of new effective technologies and materials for wastewater treatment and processing of accumulated waste. One of the alternative directions is membrane technologies, in particular, the development of biodegradable nanocomposite membranes.
Anionic polyelectrolyte – Na-carboxymethyl cellulose with molecular weight Mw ~ 90000 and cationic polyelectrolyte – low molecular weight chitosan were used to create these membranes. Polymeric membranes were formed by mixing aqueous solutions of chitosan hydrochloride and Na-carboxymethylcellulose in different ratios.
Analysis of X-ray diffractograms of polyelectrolyte complexes showed that with a gradual increase in the content of cationic polyelectrolyte from 5 % to 17 %, the structure of polyelectrolyte complexes changes dramatically. Whereas, the diffractograms of polyelectrolyte complexes with chitosan content from 29 % to 84 % are similar.
The effectiveness of the obtained membranes was tested on a barometric device at different pressures. It was established that membranes based on polyelectrolyte complexes with a stoichiometric ratio of anionic and cationic polyelectrolytes turned out to be the most productive.
The degree of water purification was checked by indicators of color selectivity. The obtained results allow us to conclude that with an increase in the duration of purification, the selectivity increases
Effects of ephedrine and phenylephrine on the Analgesia Nociception Index (ANI) and Nociception Level (NOL)
Authors: Kuroki Masahiro , Onodera Yu , Okada Masayuki , Nakane Masaki 
Published: May 2024

Abstract

Purpose: Nociceptive monitor values is thought to be influenced by vasopressors; however, no study has investigated this in detail. Therefore, this study aimed to investigate the effect of vasopressors on nociceptive monitors using Analgesia Nociception Index (ANI) and Nociception Level (NOL).
Methods: This prospective observational study was conducted between February and April, 2023. Data were obtained during anesthesia induction only to exclude surgical invasion. Blood pressure, heart rate, ANI, and NOL were measured 5 min after ephedrine or phenylephrine administration during hypotension. Low ANI and high NOL values indicate severe pain. Statistical analyses were performed on the values before and after vasopressor administration for each parameter.
Results: Twelve patients (male, 10; female, 2) with a mean age of 71.3 ± 6.7 years were included. ANI and NOL, before versus after ephedrine administration were 76.3 ± 11.8 versus 33.6 ± 8.1 (p < 0.001) and 2.1 ± 1.5 versus 27.6 ± 10.5 (p < 0.001), respectively. ANI and NOL, before versus after phenylephrine administration were 75.7 ± 9.2 versus 53.0 ± 15.3 (p = 0.010) and 4.0 ± 3.5 versus 21.5 ± 4.3 (p < 0.001), respectively. The mean time beyond the pain threshold (ANI < 50 and NOL > 25) for ephedrine and phenylephrine was 58.0 s and 21.0 s for ANI, and 29.3 s and 1.0 s for NOL, respectively.
Conclusion: The use of vasopressors, albeit a short time, significantly altered the nociceptive monitor values, and in some cases, exceeded the values considered to be the threshold for pain.
Measurable Residual Disease (MRD) dynamics in Multiple Myeloma and the influence of Clonal Diversity Analyzed by Artificial Intelligence
Authors: Martínez-López Joaquin , Lopez-Muñoz Nieves , Chari Ajai , Dorado Sara , Barrio Santiago , Arora Shagun , Kumar Anupama , Chung Alfred , Martin Thomas , Wolf Jeffrey 
Published: May 2024

Abstract

Minimal Residual Disease (MRD) assessment is a known surrogate marker for survival in multiple myeloma (MM). Here, we present a single institution’s experience assessing MRD by NGS of Ig genes and the long-term impact of depth of response as well as clonal diversity on the clinical outcome of a large population of MM patients; 482 MM patients at the University of California, San Francisco (UCSF) diagnosed from 2008 to 2020 were analyzed retrospectively. MRD assessment was performed by NGS. PFS curves were plotted by the Kaplan-Meier method. In the newly diagnosed group, 119 of 304, achieved MRD negativity at the level of 10− 6 at least once. These patients had a prolonged PFS versus patients who were persistently MRD positive at different levels (p > 0.0001). In the relapsed disease group, 64 of 178 achieved MRD negativity at 10− 6 and PFS was prolonged versus patients who remained MRD positive (p = 0.03). Three categories of MRD dynamics were defined by artificial intelligence: (A) patients with ≥ 3 consistently MRD negative samples, (B) patients with continuously declining but detectable clones, (C) patients with either increasing or a stable number of clones. Groups A and B had a more prolonged PFS than group C (p < 10− 7). Patients who were MRD positive and had not yet relapsed had a higher clonal diversity than those patients who were MRD positive and had relapsed. MRD dynamics can accurately predict disease evolution and drive clinical decision-making. Clonal Diversity could complement MRD assessment in the prediction of outcomes in MM.
Conodont stratigraphy and biodiversity of the Middle Devonian Kačàk Episode in the Pic de Bissous (Montagne Noire, S-France)
Authors: Narkiewicz Katarzyna , Narkiewicz Marek 
Published: May 2024

Abstract

Pic de Bissous belongs to classic sections of the latest Eifelian Kačàk Episode (KE) interval, uniquely developed in hemipelagic carbonate facies. The present conodont study is based on dense sampling of the section, yielding rich and diverse assemblages dominated by Polygnathus. The position of the lower and upper KE boundaries is here documented within the late Eifelian kockelianus Zone and at the base of the lowermost Givetian hemiansatus Zone, respectively. Both boundaries are marked by an increase of P1 elements diversity, the phenomenon reported also from other palaeogeographic setting, and accompanied by an appearance of several new taxa. This suggests a global two-step conodont biodiversity rise around the KE interval, presumably caused by the eustatic If and earliest Givetian sealevel rise, respectively. These events were accompanied by increased juvenile conodont mortality probably due to some local/regional adverse conditions of either abiotic or biotic nature.
An Analytical Case Study of the Oil Well Blowout at Baghjan Oil Field, Assam: a Human and Environmental Perspective
Authors: Dutta Ujjal , Kalita Shivangi 
Published: May 2024

Abstract

The study analyses the oil well blowout that took place at the Baghjan oil field in Assam, India, on 27th May 2020. This incident led to a significant fire on 9th June that lasted more than five months. The tragedy degraded the environment and inflicted substantial problems on the area's inhabitants. The present study employs the analytical case study approach and various data sources to unfold the disaster and its causes, impact, and response. It also examines the local inhabitants and environmental impact and tries to analyze the event comprehensively. The incident resulted from technical malfunctions and human errors, leading to the relocation of the adjacent settlement to refugee camps amidst the global epidemic. However, it is essential to mention that many households received adequate compensation for their damages. The incident has resulted in the contamination of the air, noise, soil, and water, causing significant damage to the fragile ecosystem and its rare species. The research employs the Normalized Difference Vegetation Index to quantify changes in vegetation cover resulting from the blowout, thus showing the extensive damage to the affected region. The incident shed light on legal and regulatory deficiencies alongside a lack of accountability and transparency within the Oil India Limited sector. Despite the numerous proposals for environmental restoration, it appears challenging to revert to the previous state swiftly. The present study reflects the collective and collaborative action to protect and preserve the environment.
Adsorptive Removal of Organic dyes by Hydrothermally Synthesized Binary Rare Earth Oxide Pr6O11: Isotherms, Optimization, Kinetic and Thermodynamic Studies.
Authors: Nayak Lipika , soren Siba , Das Nigamananda , PARHI PURNENDU 
Published: May 2024

Abstract

This study explored the potential of rare earth oxide, praseodymium oxide (Pr6O11) as an adsorbent for removing Erichrome Black T (EBT) dye from aqueous solution. Pr6O11 with a surface area (24.07 m²/g), was synthesized using hydrothermal method and characterized using various analytical techniques (XRD, SEM, TEM, BET, XPS). The influence of initial dye concentration, temperature, adsorbent dose, and pH on adsorption capacity, kinetics, and isotherms was investigated. Optimal conditions were: 60 mg/L initial dye, 0.03g adsorbent, 90 min contact time, and pH around 7–8. The heterogeneous adsorption of dyes by the prepared catalyst was best described by Freundlich adsorption isotherm model which favours multilayer adsorption of EBT dye onto Pr6O11 surface. Kinetic study confirms the progress of adsorption via pseudo-second-order kinetics. Different thermodynamic considerations such as ΔG⁰, ΔH⁰, ΔS⁰ calculated during the experiment suggested a favourable, spontaneous, and exothermic physisorption process (30–50°C). The material exhibited reusability with minimal capacity loss after each cycle.
Laparoscopic repair of parastomal hernia following radical cystectomy and ileal conduit: A Single-Center Experience
Authors: Fu Xiaojian , Hua Rong , Li Minglei , Chen Hao , Yao Qiyuan 
Published: May 2024

Abstract

Purpose: Parastomal hernia (PH) is a frequent complication following radical cystectomy and ileal conduit (IC). The purpose of this study was to summarize the clinical experience and technical characteristics of laparoscopic repair of PH following IC.
Methods: We retrospectively evaluated all patients who underwent laparoscopic treatment of PH following IC at Huashan Hospital, Fudan University from May 2013 to December 2022.
Results:Thirty-five patients were included in the study. Median follow up was 32 months. Three patients presented with a recurrence (8.6%), with a median time to recurrence of 14 months. Out of the 35 patients, Thirty-two underwent totally laparoscopic repair using the Sugarbaker technique, Three patients required open surgery to repair the intestinal injury after laparoscopic exploration. One patient died 9 months post-surgery due to COVID-19. During the follow-up period, two patients developed a peristomal abscess, and one patient experienced partial intestinal obstruction 10 days after surgery.
Conclusion: Surgical management of PH following IC is challenging. The laparoscopic Sugarbaker technique for repairing PHfollowing IC has low complication and recurrence rate.
Gender-specific effects of exposure to maternal prenatal smoking on gross motor skills and movement behaviors in 3-5 year-old children: findings from the 2012 National Youth Fitness Survey
Authors: Zhou Andrew , Qiu Zhengyuan , Zhang Chiyuan Amy 
Published: May 2024

Abstract

Background: Smoking during pregnancy has been associated with various health and behavioral problems in offspring throughout their lifespan. In addition, studies have shown that these health and behavioral problems following maternal prenatal smoking exposure (MPSE) are likely gender-dependent, i.e. the outcomes are divergent between male and female offspring. However, studies on whether MPSE affects gross motor skills in 3-5 year-old children in a gender-specific manner are lacking. The first aim of this project is to examine the effects of MPSE on gross motor skills in 3-5 year-old boys and girls. The second aim is to identify factors that are associated with gross motor skills in children with MPSE.
Methods: The data used in this project are from the 2012 National Youth Fitness Survey (NNYFS) conducted by the Centers for Disease and Prevention National Center for Health Statistics (NCHS). Out of the 368 children aged 3-5 surveyed by NCHS, 329 had locomotor skills standard score (LSSS), object control skills standard score (OCSSS), and gross motor quotient (GMQ) along with demographic data, body measurement data early childhood questionnaire data movement behavior questionnaire data and were selected for this study.
Results: Boys with MPSE had significantly lower LSSS, OCSSS, and GMQ compared to those without MPSE, while no significant differences in LSSS, OCSSSS, and GMQ for girls were detected, indicating a gender-specific effect of MPSE on GMQ in 3-5 year-old children. MPSE was associated with low ratio of family income to poverty (RFIP) in girls but not in boys, suggesting a gender-specific inverse relationship of MPSE with parity. MPSE was associated with having no physical activity outside of school for the past 7 days in boys but not in girls, watching TV for >2 hours in the past 30 days and having 2 or fewer days with physical activity at least 60 min in the past 7 days in girls but not in boys, suggest that MPSE is associated with movement behaviors that confer less physical activity in boys and more sedentary state in girls.
Conclusion: MPSE negatively impacts gross motor skills in 3-5 year-old boys.