bluuebunny/crossref_metadata_2025_split huggingface dataset:
Ecology, chorology and commonness of the Trifolium taxa in Greece
Authors: Fotiadis G. , Vrahnakis M. S. , Merou Th. , Vidakis K. 
Published: May 2010

AbstractThe taxa of the genus Trifolium L. are considered valuable genetic plant resources worldwide. The study of their chorological and floristic characteristics is of great importance. This paper aims at evaluating the taxa of the genus found in the flora of Greece in terms of their ecological, chorological and commonness characteristics. A floristic catalogue was produced based on published, but not genus‐specific, floristic data collected at various parts of the country and field surveys carried out by the authors in years 2004 and 2005. Information on the localities of the taxa, either found in the field surveys or scattered into floristic reports, was gathered. The present analysis was based on several research results and bibliographic information. It was found that the Greek flora contains 113 taxa (95 species, 17 subspecies, one hybrid) out of a total of approximately 255 species reported worldwide; six of them being Greek endemics. The taxa are distributed into two subgenera of the genus; subgenus Chronosemium including 14 species and subgenus Trifolium that includes 98 taxa (without counting one hybrid) distributed in five out of eight sections: Trifolium (56), Vesicaria (18), Trifoliastrum (15), Trichocephalum (7) and Paramesus (2). The majority of the taxa are mediterranean elements (38%), annuals (76%), therophytes (76%), and of scapose growth type (78%). It was also found that the centre of distribution of Trifolium is located in Anatolia (Turkey) and Greece, so pointing out this part of east Mediterranean as an important “gene‐pool” region. The most common Trifolium species in Greece are T. repens, T. campestre, T. fragiferum, T. physodes and T. arvense (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Seed morphology of some species of TephrosiaPers. (Fabaceae) from Saudi Arabia Identification of species and systematic significance
Authors: Al‐Ghamdi F. A. , Al‐Zahrani R. M. 
Published: May 2010

AbstractSeed morphology of eight species belonging to the genus Tephrosia Pers. were examined by using light and scanning electron microscopy. Macro‐ and micromorphological characters, including seed shape, colour, size, surface, epidermal cell shape, anticlinal boundaries, outer periclinal cell wall and relief of outer cell walls, are presented. Two types of basic anticlinal cell wall boundaries and two types of relief outer cell walls are recognized and two different shapes of the outer periclinal cell wall are described. A key for the identification of the investigated taxa based on seed characters is provided (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Cadherin 13 in cancer
Authors: Andreeva Alexandra V. , Kutuzov Mikhail A. 
Published: May 2010

AbstractWe review the evidence suggesting the involvement of Cadherin 13 (CDH13, T‐cadherin, H‐cadherin) in various cancers. CDH13 is an atypical member of the cadherin family, devoid of a transmembrane domain and anchored to the exterior surface of the plasma membrane via a glycosylphosphatidylinositol anchor. CDH13 is thought to affect cellular behavior largely through its signaling properties. It is often down‐regulated in cancerous cells. CDH13 down‐regulation has been associated with poorer prognosis in various carcinomas, such as lung, ovarian, cervical and prostate cancer. CDH13 re‐expression in most cancer cell lines inhibits cell proliferation and invasiveness, increases susceptibility to apoptosis, and reduces tumor growth in in vivo models. These properties suggest that CDH13 may represent a possible target for therapy in some cancers. At the same time, CDH13 is up‐regulated in blood vessels growing through tumors and promotes tumor neovascularization. In contrast to most cancer cell lines, CDH13 overexpression in endothelial cells promotes their proliferation and migration, and has a pro‐survival effect. We also discuss molecular mechanisms that may regulate CDH13 expression and underlie its roles in cancer. © 2010 Wiley‐Liss, Inc.
Risk factors for developing synchronous esophageal neoplasia in patients with head and neck cancer
Authors: Wang Wen‐Lun , Lee Ching‐Tai , Lee Yi‐Chia , Hwang Tzer‐Zen , Wang Chih‐Chun , Hwang Jau‐Chung , Tai Chi‐Ming , Chang Chi‐Yang , Tsai Shang‐Shyue , Wang Cheng‐Ping , Ko Jenq‐Yuh , Lin Jaw‐Town 
Published: May 2010

AbstractBackgroundThis study investigated the risk factors for synchronous esophageal neoplasia in patients with head and neck squamous cell carcinoma (HNSCC).MethodsAll 315 consecutive patients with newly diagnosed HNSCC received endoscopic esophageal screening with image‐enhanced endoscopy.ResultsSixty‐nine patients (21.9%) had synchronous esophageal neoplasia, 37 (53.6%) with superficial neoplasia and 21 (30.4%) with multiple esophageal lesions. Univariate analysis revealed age <50 years, drinking alcohol, and location of index HNSCC were significant risk factors for developing synchronous esophageal neoplasia. In multivariate analysis, drinking alcohol (odds ratio [OR], 3.792; p = .0035), index oropharynxgeal cancers (OR, 3.618; p = .0045) and hypopharyngeal cancers (OR, 2.627; p = .0029) were independent risk factors. Drinking alcohol was clearly dose–response related (p = .001).ConclusionAlcohol consumption and index tumor location are associated with the development of synchronous esophageal neoplasia in patients with HNSCC. Because of the high prevalence, routine endoscopic examination of the esophagus should be recommended, especially in patients with the risk factors identified. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Impact and management of airway obstruction in patients with squamous cell carcinoma of the larynx
Authors: Chu Pen‐Yuan , Lee Tsung‐Lun , Chang Shyue‐Yih 
Published: May 2010

AbstractBackgroundWe compared postoperative complications and oncologic results after laryngectomy of patients with laryngeal squamous cell carcinoma (SCC), with and without airway obstruction.MethodsWe retrospectively reviewed the medical records of 544 patients with laryngeal SCC between 1990 and 2000. Of 175 advanced cases receiving total laryngectomy, 32 initially presented with upper airway obstruction.ResultsPostoperative complications after laryngectomy did not differ significantly between patients with and without airway obstruction (36% vs 28%; p = .353). Although patients with airway obstruction had more T4 (81% vs 42%; p < .001) and stage IV disease (82% vs 48%; p = .0004), tumor recurrence rates did not increase (21% vs 29%; p = .374). Five‐year overall (52% vs 60%; p = .251), disease‐specific (73% vs 70%; p = .982), and relapse‐free (72% vs 68%; p = .982) survival did not differ significantly between groups.ConclusionAfter appropriate management of airway obstruction, the postoperative complications and oncologic results were similar to those without airway obstruction. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Evolving treatment strategies in thin cutaneous head and neck melanoma: 1 institution's experience
Authors: Jaber James J. , Clark Joseph I. , Muzaffar Kamil , Ruggiero Francis P. , Feustel Paul J. , Frett Michael J. , Zender Chad A. 
Published: May 2010

AbstractBackgroundAlthough existing melanoma literature provides recommendations for thinner lesions (≤1 mm) within a heterogeneous population, a focus on the head and neck group is less pervasive.MethodsThe records of 49 node‐negative individuals with thin head and neck melanoma that underwent surgical intervention ± sentinel lymph node (SLN) biopsy were reviewed.ResultsA significant increased Breslow thickness and mitotic rate, and a trend toward significance in Clark level ≥ IV were shown in patients that underwent an SLN biopsy versus those that did not. The total number of positive biopsies was 2 (5%). In our subset analysis using the modified American Joint Committee on Cancer recommendations by Wong and colleagues, the incidence of positive SLN biopsy would have increased to 11%.ConclusionWe advocate performing an SLN biopsy in thin head and neck melanomas for primary tumors > 0.75 mm, regardless of “high‐risk” features as described by Wong and colleagues. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Calpain 10 gene and laryngeal cancer: A survival analysis
Authors: Moreno‐Luna Ramón , Abrante Antonio , Esteban Francisco , González‐Moles Miguel Angel , Delgado‐Rodríguez Miguel , Sáez María Eugenia , González‐Pérez Antonio , Ramírez‐Lorca Reposo , Real Luis Miguel , Ruiz Agustín 
Published: May 2010

AbstractBackgroundLaryngeal carcinoma is a common upper respiratory tract cancer with different environmental and genetic factors involved in its development. Calpains are Ca2+‐dependent cysteine proteases that modulate cellular function. A novel association between calpain 10 (CAPN10) haplotypes and laryngeal cancer has been found recently. Therefore, the goal of this study was to analyze the contribution of CAPN10 alleles to laryngeal cancer survival.MethodsPatients were recruited from southern Spain. Genotypes were determined using pyrosequencing technology. We analyzed CAPN10 UCSNP‐44, UCSNP‐43, UCSNP‐19, and UCSNP‐63 allelic distributions in 199 patients with unrelated laryngeal cancer. Survival curves were calculated from the date of the intervention to the date of death. Multivariate analyses were done using the Cox proportional risk model.ResultsUCSNP‐19, UCSNP‐43, and UCSNP‐44 were unrelated to survival in both univariate and multivariate analyses. However, for UCSNP‐63 genotype 12 a significant relationship was observed in multivariate analysis (hazard ratio [HR] = 2.73; 95% confidence interval [CI], 1.21–6.20).ConclusionCAPN10 UCSNP‐63 genotype 12 seems to be related with a worse prognosis in laryngeal cancer. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Associations between quality of life, coping styles, optimism, and anxiety and depression in pretreatment patients with head and neck cancer
Authors: Horney Debbie J. , Smith Helen E. , McGurk Mark , Weinman John , Herold Jim , Altman Keith , Llewellyn Carrie D. 
Published: May 2010

AbstractBackgroundHead and neck cancer is often diagnosed at a late stage and consequently radical treatment is necessary. The pretreatment phase is a time of high anxiety and depression for patients. This study aimed to investigate whether anxiety and depression are related to quality of life, coping styles, and dispositional optimism.MethodsOne hundred and three patients were recruited after diagnosis to a questionnaire study. Measures included the Hospital Anxiety and Depression Scale; SF12v2 Health Survey; Brief COPE, and the Revised Life Orientation Test.ResultsQuality of life, in particular emotional role explained a large proportion of the variance in pretreatment anxiety and depression. In addition, the use of negative coping styles was related to high anxiety levels and low levels of optimism were related to higher levels of depression.ConclusionThere are a small but significant proportion of pretreatment patients that may benefit from individualized support. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Preoperative CT diagnosis of right nonrecurrent inferior laryngeal nerve
Authors: Wang Yu , Ji Qinghai , Li Duanshu , Wu Yi , Zhu Yongxue , Huang Caiping , Shen Qiang , Wang Zhuoying , Zhang Ling , Sun Tuanqi 
Published: May 2010

AbstractBackgroundThe nonrecurrent inferior laryngeal nerve is an anomaly associated with the absence of the brachiocephalic artery (BCA) and the presence of arteria lusoria.MethodsThe preoperative CT scans from 290 patients (9 nonrecurrent, 281 recurrent) were retrospectively reviewed. We identified the BCA or the arteria lusoria, and classified the relationship between the right subclavian artery (SCA) and the tracheoesophagus into 2 subtypes.ResultsThe arteria lusoria was identified in 6 nonrecurrent cases. The BCA was identified in 143 recurrent cases. The right SCA was detected on the ventral side of the membranous wall of the trachea in 273 recurrent cases, whereas it was detected on the dorsal side in 8 recurrent cases with enlarged thyroid, and it was detected on the dorsal side in 9 nonrecurrent cases without pressure sign.ConclusionsIt is possible to predict a nonrecurrent inferior laryngeal nerve by identifying the arteria lusoria or other features on the preoperative CT. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Signet ring carcinoma parotid gland: A case report
Authors: Singh Meeta , Khurana Nita , Wadhwa Rachna , Gulati Achal 
Published: May 2010

AbstractBackgroundSignet ring adenocarcinoma is a recently characterized entity occurring in the minor salivary glands. All reported cases have occurred in minor salivary gland. Pure signet ring adenocarcinoma of a major salivary gland has not been reported in the literature.MethodsA 38‐year‐old woman presented with a mass in the parotid region. Microscopy showed signet ring cells positive for mucicarmine. Immunohistochemistry showed cytokeratin and focal smooth muscle actin (SMA) positivity; an exhaustive search for primary elsewhere did not reveal any suspicious focus.ResultsA diagnosis of low‐grade signet ring adenocarcinoma of the parotid gland was made. On 6‐month follow‐up the patient was alive and well.ConclusionsThe present case highlights a rare occurrence of this tumor in the parotid gland, which until now was labeled as a tumor exclusive to minor salivary glands. Thus this entity should be kept as a differential while investigating mucinous tumors of parotid. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Small Molecular Compounds that Inhibit Hepatitis C Virus Replication Through Destabilizing Heat Shock Cognate 70 Messenger RNA†
Authors: Peng Zong-Gen , Fan Bo , Du Na-Na , Wang Yu-Ping , Gao Li-Mei , Li Ying-Hong , Li Yu-Huan , Liu Fei , You Xue-Fu , Han Yan-Xing , Zhao Zhi-Yun , Cen Shan , Li Jian-Rui , Song Dan-Qing , Jiang Jian-Dong 
Published: May 2010

Abstract

Host heat shock cognate 70 (Hsc70) protein is packaged into hepatitis C viral (HCV) particles as a structural component of the virus in the assembly process. It helps HCV RNA release into the cytoplasm in the next infection cycle. The goal of this study is to investigate whether chemically down-regulating host Hsc70 expression could be a novel strategy to interrupt HCV replication. Compounds were screened with an Hsc70 messenger RNA (mRNA) assay. IMB-DM122 was found to be an effective and safe inhibitor for Hsc70 mRNA/protein expression in human hepatocytes. IMB-DM122 inhibited HCV replication through destabilization of Hsc70 mRNA, and the half-life of host Hsc70 mRNA was reduced by 78% after the compound treatment. The Hsc70 mRNA 3′ untranslated region sequence is the element responsible for the effect of IMB-DM122 on Hsc70 mRNA. The compound appears to be highly efficient in inhibiting Hsc70-related HCV replication. Treatment of the HCV-infected hepatocytes with IMB-DM122 reduced the virion encapsidation of Hsc70, and therefore disrupted HCV replication and the infection cycle. IMB-DM122 showed considerable good safety

in vitro

as well as

in vivo

with no indication of harmful effect on liver and kidney functions.

Conclusion:

Hsc70 might be a new drug target and mechanism to inhibit HCV proliferation.
Delineating low‐count defective‐contour SPECT lung scans for PE diagnosis using adaptive dual exponential thresholding and active contours
Authors: Wang Alex , Yan Hong 
Published: May 2010

AbstractTo develop an automated lung delineation method that greatly improves the accuracy of contour extraction in SPECT lung scans with low maximum count, total counts, and/or defective contour edges, by combining deformable contour and planar image processing techniques with our existing adaptive dual exponential thresholding method. Two datasets consisting of 90 Monte Carlo simulations and 35 SPECT scans of real patients both with low maximum/total counts were used as the basis of this study. After preliminary contour extraction using dual exponential thresholding, a deformable contour algorithm was applied, combined with edge enhancement using image processing techniques such as Gaussian transformation and Sobel edge detection. Both quantitative validation via statistical measures and qualitative verification by an experienced practitioner were implemented to evaluate the method. An average of 83% congruency was achieved for 45 simulations with maximum count values below 31. This is a significant 33% improvement on the results of our previous study using dual exponential thresholding alone. Overall congruence averaged 95% for the entire simulation dataset. With the set of 35 actual SPECT scans with relatively low maximum/total counts, congruency again averaged near 95% with a few outliers ranging between 75 and 90%. We have successfully combined active contour with adaptive dual exponential thresholding to delineate lung contours accurately for SPECT scans with low maximum/total counts and/or defective contours due to various cardiopulmonary disorders. © 2010 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 20, 149–154, 2010
Computer‐based morphometry of brain
Authors: Koo Bang‐Bon , Kim Dae‐Shik 
Published: May 2010

AbstractOver the past decade, the importance of probing the anatomy of the brain has reemerged as an important field of neuroscience. In combination with functional imaging techniques, the rapid advancement of neuroimaging techniques—such as magnetic resonance imaging—and their growing applicability in studying brain morphometry has led to great advances in neuroscience research. Considering the requirements of the diverse technologies—from image processing to statistics—in performing morphometry of the brain, it is critical to have an overall understanding of this subject. The major objective of this review is to provide a practical introduction to this field. The review starts by covering basic concepts and techniques that are commonly used in morphometry of structural magnetic resonance imaging and then extends to further technical perspectives. © 2010 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 20, 117–125, 2010
Protein adsorption on biodegradable polyanhydride microparticles
Authors: Carrillo‐Conde Brenda , Garza Alicia , Anderegg James , Narasimhan Balaji 
Published: May 2010

AbstractThe in vitro adsorption of plasma proteins on polyanhydride microparticles based on sebacic acid (SA), 1,6‐bis(p‐carboxyphenoxy)hexane (CPH), and 1,8‐bis(p‐carboxyphenoxy)‐3,6‐dioxaoctane (CPTEG) was studied. Three model proteins from bovine serum (albumin (BSA), immunoglobulin G (IgG), and fibrinogen (Fg)) were used. The adsorption was studied using X‐Ray Photoelectron Spectroscopy and gel electrophoresis. 2D electrophoresis was used to study the adsorption of plasma proteins from bovine serum. Differences in the amount of protein adsorbed were detected as a function of the following: (i) copolymer composition and (ii) specific protein studied. A direct correlation between polymer hydrophobicity and protein adsorbed was observed and higher quantities of Fg and IgG were absorbed. In vitro release studies were performed with ovalbumin‐encapsulated microparticles that were incubated with Fg; these studies showed a reduction in the amount of ovalbumin released from the microparticles when Fg is adsorbed on the surface. An understanding of protein adsorption patterns on parenteral delivery devices is valuable in optimizing their in vivo performance. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010
Nicotinamide phosphoribosyltransferase/sirtuin 1 pathway is involved in human immunodeficiency virus type 1 Tat‐mediated long terminal repeat transactivation
Authors: Zhang Hong‐Sheng , Sang Wei‐Wei , Wang Yu‐Ou , Liu Wei 
Published: May 2010

AbstractTat is a multifunctional transactivator encoded by human immunodeficiency virus type 1 (HIV‐1). Tat transactivating activity is controlled by nicotinamide adenine nucleotide+ (NAD+)‐dependent deacetylase sirtuin 1 (SIRT1). Nicotinamide phosphoribosyltransferase (Nampt) is a rate‐limiting enzyme in the conversion of nicotinamide into NAD+, which is crucial for SIRT1 activation. Thus, the effect of Nampt on Tat‐regulated SIRT activity was studied in Hela‐CD4‐β‐gal (MAGI) cells. We demonstrated that Tat caused NAD+ depletion and inhibited Nampt mRNA and protein expression in MAGI cells. Resveratrol reversed Tat‐induced NAD+ depletion and inhibition of Nampt mRNA and protein expression. Further investigation revealed that Tat‐induced inhibition of SIRT1 activity was potentiated in Nampt‐knockdown by Nampt siRNA compared to treatment with Tat alone. Nampt siRNA potentiated Tat‐induced HIV‐1 transactivation in MAGI cells. Altogether, these results indicate that Nampt is critical in the regulation of Tat‐induced inhibition of SIRT1 activity and long terminal repeat (LTR) transactivation. Nampt/SIRT1 pathway could be a novel therapeutic tool for the treatment of HIV‐1 infection. J. Cell. Biochem. 110: 1464–1470, 2010. © 2010 Wiley‐Liss, Inc.
Extracellular high K+ stimulates vesicular glutamate release from astrocytes by activating voltage‐dependent calcium channels
Authors: Yaguchi Takahiro , Nishizaki Tomoyuki 
Published: May 2010

AbstractExtracellular high K+ (75 mM) increased intracellular Ca2+ concentrations in cultured rat hippocampal astrocytes, and the Ca2+ rise was abolished by deleting extracellular Ca2+ or cadmium, a non‐selective inhibitor of voltage‐dependent calcium channels (VDCCs). In the reverse transcription‐polymerase chain reaction analysis, cultured astrocytes expressed mRNAs for L type‐VDCC subunits such as α1B, α1C, α1D, and α1E. Extracellular high K+ (75 mM) stimulated glutamate release from astrocytes. The glutamate release was not prevented by the glutamate transporter inhibitor, L‐trans‐pyrrolidine‐2,4‐dicarboxylic acid (PDC), or deleting extracellular Na+, but otherwise it was clearly inhibited by deleting extracellular Ca2+, cadmium, vesicular transport inhibitors such as brefeldin A, bafilomycin A1, and latrunculin B, or botulinum toxin‐A, an exocytosis inhibitor. Extracellular high K+ (75 mM) bleached fluorescent signals of FM1‐43, taken up into the vesicular membrane in astrocytes, that was also inhibited by deleting extracellular Ca2+, cadmium, brefeldin A, bafilomycin A1, latrunculin B, or botulinum toxin‐A, but not by PDC. Taken together, the results of the present study indicate that extracellular high K+‐evoked depolarization activates VDCCs expressed in astrocytes, causing an increase in intracellular Ca2+ concentrations through VDCCs, which triggers vesicular glutamate release from astrocytes, independently of reverse transport through glutamate transporters. J. Cell. Physiol. 225: 512–518, 2010. © 2010 Wiley‐Liss, Inc.