bluuebunny/crossref_metadata_2025_split huggingface dataset:
Arm disability in patients on hemodialysis
Authors: Toffola Elena Dalla , Petrucci Lucia , Mazzacane Bruno , Klersy Catherine , Villa Giuseppe , Bossi Daniela , Bejor Maurizio 
Published: March 2010

AbstractINTRODUCTIONEnd‐stage renal disease patients on hemodialysis (HD) often present osteoarticular and musculoskeletal complications related both to chronic renal disease as well as to chronic dialysis. The aim of the present study was to assess the degree of upper‐limb disability, and its determinants, in a group of patients on chronic HD to determine functional capacity and the need for rehabilitative treatment.MATERIALS AND METHODSWe studied 97 HD patients (46 male, 51 female) with a mean age of 64.4 years, and 51 healthy controls. The Disabilities of the Arm, Shoulder, and Hand (DASH) Outcome Measure was used to clinically assess degree of disability as well as finger function and movement.RESULTSFor study purposes, patients were divided into 3 groups according to the duration of dialysis: <5 years, 5–10 years, and >10 years. The median DASH score was 9.5 (interquartile range [IQR]: 3.4–24.1) in the entire population. However, it differed significantly in the 3 dialysis groups with scores of 5.2 (IQR: 2.6–18.1), 14.7 (3.4–23.7), and 37.5 (9.5–73.3) in the <5 year, 5–10 year, and >10 year groups, respectively (p < .001). Additionally, disability correlated with shoulder and hand pain and with finger stiffness. Moreover, patients on dialysis for more than 10 years were affected by pain involving several upper limb joints. All patients who worked had a DASH score lower than 20. The median DASH score was 2.6 (IQR: 0.9–5.2) in the control group.CONCLUSIONDuration of dialysis is associated with progressive disability. Thus, early rehabilitation programs are recommended in patients who are expected to undergo long‐term dialysis.
A more cost effective and rapid high percentage germ‐line transmitting chimeric mouse generation procedure via microinjection of 2‐cell, 4‐cell, and 8‐cell embryos with ES and iPS cells
Authors: Kraus Petra , Leong Geraldine , Tan Valerie , Xing Xing , Goh Jie Wei , Yap Sook Peng , Lufkin Thomas 
Published: March 2010

AbstractThe long‐standing traditional method of delivering embryonic stem (ES) cells adjacent to the inner cell mass (ICM) of blastocysts to generate chimeras improved with the advent of laser‐ or Piezo assisted 8‐cell embryo microinjection. Building on this technology but omitting either the laser or the Piezo to penetrate the zona pellucida and making use of earlier embryonic stages (2‐cell and 4‐cell), we were able to significantly speed up and economize our ES cell microinjection and chimera production throughput. We demonstrate here that embryonic (ES) and induced pluripotent stem (iPS) cells can stay fully pluripotent when delivered into 2‐cell‐ and 4‐cell‐stage embryos, long before they would naturally be incorporated into the ICM of a blastocyst (E3.5) and give rise to high percentage and germline transmitting chimeras. genesis 48:394–399, 2010. © 2010 Wiley‐Liss, Inc.
Synthesis, Crystal Structures, and Magnetic Properties of MnII, CoII, and ZnII Coordination Polymers Containing 1,2,4,5‐Benzenetetracarboxylic Acid and 4,4′‐Azobispyridine
Authors: Zhao Li‐Min , Li Huan‐Huan , Wu Ying , Zhang Shi‐Yuan , Zhang Zhen‐Jie , Shi Wei , Cheng Peng , Liao Dai‐Zheng , Yan Shi‐Ping 
Published: March 2010

AbstractManganese(II), cobalt(II), and zinc(II) coordination polymers, {[Mn(H2btec)(azopy)(H2O)2]·(azopy)}n (1), {[Co(H2btec)(azopy)(H2O)2]·(azopy)}n (2), and {[Zn2.5(btec)(azopy)(μ3‐OH)(H2O)]·H2O}n (3) (H4btec = 1,2,4,5‐benzenetetracarboxylic acid; azopy = 4,4′‐azobispyridine), were synthesized by ahydrothermal method and characterized by single‐crystal X‐ray diffraction, elemental analysis, and powder X‐ray diffraction. Compounds 1 and 2 are isomorphic and crystallize in the triclinic space group P$\bar {1}$, exhibiting a 2D grid framework bridged by two types of ligands. Compound 3 also crystallizes in the triclinic space group P$\bar {1}$ and possess an interesting 3D coordination framework with an unprecedented dodecadentate coordination mode and a unique {416.510.618.7}{44.5.6}2 topological structure. The ideal topology of 3 has not been encountered in metal–organic framework chemistry, which has been deposited in the Reticular Chemistry Structure Resource under the name zim net. Magnetic studies show that there are antiferromagnetic interactions in both 1 and 2. The cyclic voltammograms of 1–3 in the solid state were also obtained.
Dicarba‐closo‐dodecaboranes with One and Two Ethynyl Groups Bonded to Boron
Authors: Himmelspach Alexander , Finze Maik 
Published: March 2010

AbstractThe diethynyldicarba‐closo‐dodecaboranes 1,2‐R2‐9,12‐(HCC)2‐closo‐1,2‐C2B10H8 [R = H (1a), Me (2a)] and 9,10‐(HCC)2‐closo‐1,7‐C2B10H10 (3a) were obtained by Pd‐catalyzed Kumada‐type cross‐coupling reactions of the corresponding diiodinated dicarba‐closo‐dodecaboranes withMe3SiCCMgBr followed by desilylation of the trimethylsilylalkynyl‐substituted clusters. In addition, the related {closo‐C2B10} derivatives with one ethynyl group 1,2‐R‐9‐HCC‐closo‐1,2‐C2B10H9 [R = H (4a), Me (5a)], 9‐HCC‐closo‐1,7‐C2B10H11 (6a), and 2‐HCC‐closo‐1,12‐C2B10H11 (7a) were synthesized and their spectroscopic properties were compared to those of the diethynyl‐substituted {closo‐C2B10} clusters. The ethynyl‐ and trimethylsilylalkynyl‐functionalized dicarba‐closo‐dodecaboranes were characterized by elemental analysis, mass spectrometry, as well as by multinuclear NMR, IR, and Raman spectroscopy. The assignment of the NMR spectroscopic chemical shifts and the IR and Raman bands is supported by theoretical values derived from density functional calculations. The crystal structures of 9,12‐(HCC)2‐closo‐1,2‐C2B10H10 (1a), 9,10‐(HCC)2‐closo‐1,7‐C2B10H10 (3a), and 1,2‐Me‐9,12‐(Me3SiCC)2‐closo‐1,2‐C2B10H8 (2b) were determined by single‐crystal X‐ray diffraction. Selected experimental bond properties are compared to bond lengths and angles calculated at the B3LYP/6‐311++G(d,p) level of theory.
A Structural Investigation of Trivalent and Divalent Rare Earth Thiocyanate Complexes Synthesised by Redox Transmetallation
Authors: Bakker Jacinta M. , Deacon Glen B. , Forsyth Craig M. , Junk Peter C. , Wiecko Michal 
Published: March 2010

AbstractA wide range of reactive trivalent and divalent rare earth thiocyanate complexes with 1,2‐dimethoxyethane (dme), tetrahydrofuran (thf) and acetonitrile namely [RE(NCS)3(dme)3] [RE = La (1a), Nd (1b)], [RE(NCS)3(dme)2(μ‐dme)0.5]2 [RE = Dy (1d), Er (1e)], [RE(NCS)3(thf)4]2 [RE = Y (3a), La (3b), Nd (3c), Sm (3d), Dy (3e), Ho (3f)], [Ca(NCS)2(dme)3] [rac‐(4a)], [RE(NCS)2(dme)3] (RE = Sm (rac‐4b), Eu (rac‐4c), [Ca(Yb)(NCS)2(NCMe)5]·NCMe [Ca (6a), Yb (6b)], and [Eu(NCS)2(thf)4]2 (7) have been prepared in good to high yield by redox transmetallation between rare earth metals and the commercially available Hg(SCN)2. In addition [Eu(NCS)3(dme)2(μ‐dme)0.5]2 (1c) and [Yb(NCS)3(dme)2] (1f) have been prepared by oxidation of rac‐4c and [Yb(NCS)2(thf)2], respectively, with Hg(SCN)2 in dme. Crystallisation of 1e from dme/diglyme (diglyme = bis(2‐methoxyethyl) ether) and thf yields [Er(NCS)3(diglyme)(dme)] (2) and [Er(NCS)3(thf)4]2 (3g), respectively, and prolonged storage of rac‐4a in cold dme gave [Ca(NCS)2(dme)2(H2O)] (5). All undergo rapid hydrolysis on exposure to air. Two structural breaks are observed for [RE(NCS)3(dme)n] complexes, all of which have solely RE–NCS binding. Compounds 1a,b are nine‐coordinate monomers that differ in the arrangement (T or Y) of the RE(NCS)3 moiety, 1c–e are eight coordinate dinuclear complexes being bridged by a single η1:η1‐(O,O′)‐dme and 1f is a seven coordinate monomer. 2 is an eight coordinate monomer with, unusually for the present complexes, a facial RE(NCS)3 array. By contrast 3a–g are all dimeric, eight coordinate complexes with two unusual RE–NCS–RE bridges. The RE–S bonds are very weak and show little variation with RE3+ radius, and Er–S of 3g increases by 0.18 Å between 123 K and room temperature), foreshadowing a change to a seven coordinate monomer with [Yb(NCS)3(thf)4]. Monomeric eight coordination with transoid (N–RE–N ≈ 149°) SCN–RE–NCS bonding is observed in rac‐4a–c and Λ‐4c, whilst 5 is a seven‐coordinate monomer with a cis‐Ca(NCS)2 array. Single crystals of 6,a,b contain seven‐coordinate, pentagonal bipyramidal monomers with trans‐NCS ligands, whereas 7 is dimeric with two Eu–NCS–Eu bridging ligands and seven coordination for Eu.
Peak parking determination of the obstruction factor in lauryl acrylate monolithic CEC columns
Authors: Anderson Gwendolyn J. , LaPier Zoe , Cammarata Michael B. , Cullum Tae Sun , Bushey Michelle M. 
Published: March 2010

AbstractThe peak parking method was used to determine the obstruction factor of lauryl acrylate porous polymer monoliths. Polymers were prepared in situ in fused‐silica capillaries using thermally initiated polymerization. These columns have been used for CEC of neutral analytes. Thiourea, which is unretained, was used as the test analyte for the obstruction factor measurement. The obstruction factor was determined to be 0.72 with a SD of (±0.01), which is consistent with the concept that organic porous polymer monoliths are more permeable than traditional LC stationary phases.
New capillary coatings in open tubular CEC as models for biological membranes
Authors: Martma Kert , Fernaeus Sandra Zetterström , Land Tiit , Shimmo Ruth 
Published: March 2010

AbstractNovel stationary phases in open tubular CEC were investigated. The coating procedure was fast and simple. The coating material contained membrane suspension of different neuronal cell lines. The performance and stability of three cell lines: human neuroblastoma SH‐SY5Y, murine microglia Bv‐2 and human glioma U87‐MG cells were studied. The coating solution was expected to contain both membrane proteins and membrane lipids. The presence of membrane proteins was tested by Western blotting and the presence of phospholipids by the analysis of phosphorus content. The stability of the coating was estimated by monitoring the mobility of EOF over successive runs. The effects of pH, storage time and temperature on the coating stability were also studied. The results showed that the cell membrane‐based coating was stable over pH range of 6.5–8.5. Coatings derived from different cells yielded similar stability and EOF mobility. Capillary coated with a membrane solution was stable over 3‐day period. The same coating solution could be used for 3 weeks.
Simultaneous analysis of multiple PCR amplicons enhances capillary SSCP discrimination of MHC alleles
Authors: Alcaide Miguel , López Lidia , Tanferna Alessandro , Blas Julio , Sergio Fabrizio , Hiraldo Fernando 
Published: March 2010

AbstractMajor histocompatibility complex (MHC) genotyping still remains one of the most challenging issues for evolutionary ecologists. To date, none of the proposed methods have proven to be perfect, and all provide both important pros and cons. Although denaturing capillary electrophoresis has become a popular alternative, allele identification commonly relies upon conformational polymorphisms of two single‐stranded DNA molecules at the most. Using the MHC class II (β chain, exon 2) of the black kite (Aves: Accipitridae) as our model system, we show that the simultaneous analysis of overlapping PCR amplicons from the same target region substantially enhances allele discrimination. To cover this aim, we designed a multiplex PCR capable to generate four differentially sized and labeled amplicons from the same allele. Informative peaks to assist allele calling then fourfold those generated by the analysis of single PCR amplicons. Our approach proved successful to differentiate all the alleles (N=13) isolated from eight unrelated birds at a single optimal run temperature and electrophoretic conditions. In particular, we emphasize that this approach may constitute a straightforward and cost‐effective alternative for the genotyping of single or duplicated MHC genes displaying low to moderate sets of divergent alleles.
Separation of propranolol enantiomers by CE using sulfated β‐CD derivatives in aqueous and non‐aqueous electrolytes: Comparative CE and NMR study
Authors: Servais Anne‐Catherine , Rousseau Anne , Fillet Marianne , Lomsadze Ketevan , Salgado Antonio , Crommen Jacques , Chankvetadze Bezhan 
Published: March 2010

AbstractSeparations using CE employing non‐aqueous BGE are already as well established as separations in aqueous buffers. The separation mechanisms in achiral CE with non‐aqueous BGEs are most likely similar to those in aqueous buffers. However, for the separation of enantiomers involving their interaction with chiral buffer additives, the interaction mechanisms might be very different in aqueous and non‐aqueous BGEs. While the hypothesis regarding distinct mechanisms of enantiomer separations in aqueous and non‐aqueous BGEs has been mentioned in several papers, no direct proof of this hypothesis has been reported to date. In the present study, the enantiomers of propranolol were resolved using CE in aqueous and non‐aqueous methanolic BGEs with two single isomer sulfated derivatives of β‐CD, namely heptakis (2,3‐diacetyl‐6‐sulfo)‐β‐CD and heptakis (2,3‐dimethyl‐6‐sulfo)‐β‐CD. The enantiomer migration order of propranolol was inverted when an aqueous BGE was replaced with non‐aqueous BGE in the case of heptakis (2,3‐dimethyl‐6‐sulfo)‐β‐CD but remained the same in the case of heptakis (2,3‐diacetyl‐6‐sulfo)‐β‐CD. The possible molecular mechanisms leading to this reversal of enantiomer migration order were studied by using nuclear overhauser effect spectroscopy in both aqueous and non‐aqueous BGEs.
Enhanced selectivity in CZE multi‐chiral selector enantioseparation systems: Proposed separation mechanism
Authors: Dubský Pavel , Svobodová Jana , Tesařová Eva , Gaš Bohuslav 
Published: March 2010

AbstractIt has been reported many times that the commercial mixtures of chiral selectors (CS), namely highly sulfated β‐CDs (HS‐β‐CDs), provide remarkable enantioselectivity in CZE when compared with single‐isomer CDs, even single‐isomer HS‐β‐CDs. This enhanced enantioselectivity of multi‐CS enantioseparative CZE is discussed in the light of multi‐CS model that we have introduced earlier. It is proposed on a theoretical basis and verified experimentally that the two enantiomers of a chiral analyte under interaction with a mixture of CSs are very likely to differ in their limit mobilities, which is opposite to single‐CS systems where the two limit mobilities are likely to be the same. Thus while the enantioseparation is usually controlled by different distribution constants between the two enantiomers and CS used in single‐CS systems, an additional, electrophoretic, enantioselective mechanism resulting from different limit mobilities may play a significant role in multi‐CS systems. This additional mechanism generally makes the multi‐CS systems more selective than the single‐CS systems. The possible inequality of limit mobilities is also significant for optimization of separation conditions using mixtures of CSs. A practical example supporting our considerations is shown on enantioseparation of lorazepam in the presence of a commercial mixture of HS‐β‐CDs and a single‐isomer HS‐β‐CD, heptakis(6‐O‐sulfo)‐β‐CD.
Determination of free and total levels of phenytoin in human plasma from patients with epilepsy by MEKC: An adequate alternative to HPLC
Authors: Lin Ping‐Chih , Hsieh Ya‐Hui , Liao Fen‐Fen , Chen Su‐Hwei 
Published: March 2010

AbstractPhenytoin is a widely used anti‐seizure agent, and a good correlation is observed between its concentration in plasma and the clinical effect. We developed a selective CE with UV detection at 200 nm for analysis of free and total levels of phenytoin in human plasma based on MEKC. A sample pretreatment by liquid–liquid extraction with ethyl acetate for determination of total level of phenytoin and serum ultrafiltrate was prepared by ultrafiltration technique (ultrafiltration membrane 30 kDa; 2000 g, 10 min) for determination of free level of phenytoin and subsequent quantification by MEKC was used. MEKC was performed in Tris buffer (25 mM; pH 10.5) containing SDS (180 mM) and EG (13%) as BGE. Hydrodynamic injection for phenytoin determination (0.5 psi 5 s for total level, 2 psi 5 s for free level) was used to introduce samples. The separation voltage was set at 20 kV. Data obtained by MEKC were compared with the results by a validated HPLC method. The MEKC assay of phenytoin exhibited a very good correlation with respect to HPLC by Bland–Altman method. The equations for the Passing–Bablok regression line were as follows: for total level: MEKC=1.0143×HPLC+0.0976, r2=1; for free level: MEKC=1.0013×HPLC–0.0016, r2=1. The proposed method was applied successfully to monitor free and total levels of phentoin in 20 patients with seizures.
Efficient implementation of the proper outlet flow conditions in blood flow simulations through asymmetric arterial bifurcations
Authors: Johnson David A. , Naik Ulhas P. , Beris Antony N. 
Published: March 2010

AbstractWe present an efficient implementation of the proper (in vivo) outlet boundary conditions in detailed, three‐dimensional (3D) and time‐periodic simulations of blood flow through arteries. This is achieved through the intermediate use of an approximate ‘simulant’ model of the outlet pressure/flow relationship corresponding to the full 3D and time‐dependent numerical simulation. This model allows us to efficiently couple the 3D outlet pressure/flow conditions to the equivalent relations due to the downstream arterial network, as obtained from a one‐dimensional approximate model in the form of Fourier frequency impedance coefficients. An adjustable time‐periodic function correction term in the simulant model requires input from the full 3D model that has to run iteratively until convergence. The advantage of the proposed numerical scheme is that it decouples the upstream detailed simulation from the downstream approximate network model offering exceptional versatility. This approach is demonstrated here in a series of detailed 3D simulations of blood flow, performed using the commercial software FLUENT™, through an asymmetric arterial bifurcation. Two cases are considered: first a healthy system patterned after the left main coronary arterial bifurcation, and second a diseased case where an occlusion has developed in one of the daughter vessels, resulting in strengthening the asymmetry of the bifurcation. Rapid convergence of the iterative process was achieved in both cases. Subtle changes occur in the shear patterns of the daughter vessels, whereas the flow distribution is quite different. In the presence of a stenosis additional regions of low shear develop due to inertial effects. Copyright © 2010 John Wiley & Sons, Ltd.
Temoporfin‐mediated photodynamic therapy in patients with advanced, incurable head and neck cancer: A multicenter study
Authors: Tan Ing Bing , Dolivet Gilles , Ceruse Philippe , Poorten Vincent Vander , Roest Gerda , Rauschning Winrich 
Published: March 2010

AbstractBackground.The aim of this study was to confirm the efficacy of temoporfin (Foscan)‐mediated photodynamic therapy (PDT) in patients with end‐stage head and neck squamous cell carcinoma (HNSCC).Methods.Thirty‐nine patients with recurring HNSCC lesions ≤10 mm in depth were injected with temoporfin, followed by illumination of the tumor surface.Results.Of 39 treated patients, 19 achieved complete response, 2 partial response, 5 stable disease, 5 had progressive disease, and 8 patients were nonevaluable. Thus, in the per‐protocol analysis, the response rate was 68%. Of the treated patients 54% had a response. Median survival was significantly longer for responders (37 months) than for nonresponders (7.4 months). Nine patients were alive at 3.7 to 6.5 years (median, 4.8 years) post‐treatment, 7 free of disease. No major toxicities were observed.Conclusions.Patients with advanced HNSCC with lesions ≤10 mm in depth, who have exhausted other treatment options, can achieve significant local control and survival benefit from temoporfin‐mediated PDT. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Mastoid recurrence after radiotherapy for nasopharyngeal carcinoma: Two case studies
Authors: Zhang Ximei , Luo Jingwei , Gao Li , Xu Guozhen 
Published: March 2010

AbstractBackground.The mastoid is a rare site of nasopharyngeal carcinoma recurrence after radiotherapy, and no relevant reports are currently in the literature.Methods.Two case reports are presented describing patients with a history of nasopharyngeal carcinoma who received primary radical radiotherapy. The first was treated by conventional radiotherapy, using 2‐dimensional techniques, while the second was delivered by intensity‐modulated radiation therapy (IMRT).Results.The patients presented with progressive mastoid recurrence after radiotherapy at 12 and 16 months, respectively. Clinical presentation of mastoid recurrence was similar to mastoiditis. Meanwhile, distant metastasis occurred in both cases. To date, 1 has died of distant metastasis, and the other is alive with disease stabilization following chemotherapy treatment.Conclusion.This is the first report of mastoid recurrence after radiotherapy for nasopharyngeal carcinoma, which should alert clinicians to probe into the pathogenesis and pay attention to the relationship between mastoid recurrence and distant metastasis. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
SPECT/CT for sentinel lymph node mapping in head and neck melanoma
Authors: Vermeeren Lenka , Valdés Olmos Renato A. , Klop W. Martin C. , van der Ploeg Iris M. C. , Nieweg Omgo E. , Balm Alfons J. M. , van den Brekel Michiel W. M. 
Published: March 2010

AbstractBackground.The additional value of single photon emission computed tomography with CT (SPECT/CT) for detection and localization of sentinel nodes in patients with a melanoma of the head and neck was determined.Methods.Thirty‐eight patients received conventional lymphoscintigraphy followed by hybrid SPECT/CT. The number of sentinel nodes visualized and anatomic information provided were analyzed. Changes in surgical approach due to additional information from the SPECT/CT were evaluated in 20 patients.Results.SPECT/CT visualized a mean of 2.6 sentinel nodes per patient (range, 1–6). SPECT/CT depicted an additional sentinel node in 16% of the patients and clearly showed the anatomic location of the hot nodes in all patients. The surgical approach was adjusted on the basis of SPECT/CT images in 11 patients (55%).Conclusion.SPECT/CT visualizes more sentinel nodes than conventional images and shows their anatomic location. SPECT/CT is recommended in patients with a melanoma in the head or neck. © 2010 Wiley Periodicals, Inc. Head Neck, 2011