bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thrombosis, fracture, and percutaneous removal of a patent foramen ovale closure device 1 month after successful deployment
Authors: Gori Tommaso , Schräder Rainer , Genth‐Zotz Sabine 
Published: January 2010

AbstractSeveral different devices have been developed for the percutaneous closure of interatrial defects and patent foramen ovale. Although the implantation of these devices is both safe and effective, a number of complications, both in the early and the late follow‐up, may occur. We describe a case of device fracture manifested early (1 month after implantation) with the formation of massive thrombosis on the right atrial disc. The patient was treated with anticoagulants and the device was percutaneously retrieved. Our images allowed early noninvasive therapy and emphasize the need for echocardiographic follow‐up early after implantation. © 2010 Wiley‐Liss, Inc.
Amplatzer vascular plugs for occlusion of a left internal mammary artery graft anastomosed to the anterior interventricular vein
Authors: Badhey Neeraj , Banerjee Subhash , Brilakis Emmanouil S. 
Published: January 2010

AbstractThe authors present a case of left internal mammary artery graft anastomosis to the anterior interventricular vein leading to congestive heart failure. The patient underwent successful left main stenting using an Impella left ventricular assist device and left internal mammary graft occlusion using two Amplatzer vascular plugs. © 2010 Wiley‐Liss, Inc.
IVUS radiofrequency analysis in the evaluation of the polymeric struts of the bioabsorbable everolimus‐eluting device during the bioabsorption process
Authors: Sarno Giovanna , Onuma Yoshinobu , Garcia Hector M. Garcia , Garg Scot , Regar Evelyn , Thuesen Leif , Dudek Dariusz , Veldhof Susan , Dorange Cecile , Ormiston John A. , Serruys Patrick W. 
Published: January 2010

AbstractBackground: In the ABSORB study cohort A the changes in the amount of dense calcium and necrotic core have not been reported in comparison to the prestenting phase; this evaluation could be useful to better clarify the bioabsorption process. Aim of this study was therefore to evaluate the dynamic changes in plaque size and plaque tissue composition observed between 6 months and 2 years follow‐up, and to compare these findings to the prestenting phase. Methods: Angiography, intravascular ultrasound and derived parameters (virtual histology, palpography, and echogenicity) were serially assessed postprocedure, at 6 months and at 2 years in 20 patients. In a subset of 8 patients the same measurements were also recorded in the prestenting phase. Results: In the total population a reduction of 18% in the plaque area was observed between 6 month and 2 year follow‐up (7.56 ± 2.32 mm2 at 6 months vs. 6.16 ± 2.10 mm2 at 2 year follow‐up; P < 0.01). In the subgroup of eight patients who underwent IVUS during the pre‐stenting phase, the plaque area at 2 year follow‐up was not significantly different when compared to the prestenting plaque area (7.29 ± 2.29 mm2 at prestenting vs. 7.48 ± 1.45 mm2 at 2 year follow‐up, P = NS). Necrotic core area was reduced by 24% between the 6 month and 2 year follow‐up (0.97 ± 0.66 mm2 at 6 months vs. 0.74 ± 0.53 mm2 at 2 year follow‐up; P = NS), whilst dense calcium was reduced by 14% from 6 month to 2 year follow‐up (0.83 ± 0.50 mm2 at 6 months vs 0.72 ± 0.64 mm2 at 2 year follow‐up; P = NS). Whilst the necrotic core at 2 years follow‐up was not significantly different when compared to the pre‐stenting phase (0.62 ± 0.42 mm2 prestenting vs 1.07 ± 0.56 mm2 at 2 year follow‐up; P = NS), the area of dense calcium was significantly higher at follow‐up compared to prestenting (0.35 ± 0.35 mm2 pre‐stenting vs. 0.84 ± 0.66 mm2 at 2 year follow‐up; P < 0.05). Conclusions: The reduction in the necrotic core component between 6 month and two year follow‐up could be related to a synergistic effect of the bio‐absorption process and the anti‐inflammatory action of everolimus. © 2010 Wiley‐Liss, Inc.
Liquid‐Phase Hydrogenation of Unsaturated Aldehydes: Enhancing Selectivity of Multiwalled Carbon Nanotube‐Supported Catalysts by Thermal Activation
Authors: Machado Bruno F. , Gomes Helder T. , Serp Philippe , Kalck Philippe , Faria Joaquim L. 
Published: January 2010

AbstractPlatinum and iridium organometallic precursors are used to prepare nanosized, thermally stable multiwalled carbon nanotube‐supported catalysts. The materials are characterized by N2 adsorption at 77 K, temperature‐programmed desorption coupled with mass spectrometry, H2 chemisorption, transmission electron microscopy and thermogravimetric analysis; they are tested in the selective hydrogenation of cinnamaldehyde to cinnamyl alcohol under mild conditions (363 K and 1 MPa). A thermal activation at 973 K is found to have a very positive effect over both activity and selectivity, leading to selectivities of approximately 70 %, at 50 % conversion, regardless of the active metal phase (Pt or Ir). Since no noticeable differences in the metal particle sizes are detected, the results are interpreted in light of an enhanced metal/support interaction. This effect, induced by the removal of oxygenated surface groups, is thought to change the adsorption mechanism of the cinnamaldehyde molecule.
Design, Synthesis and Biological Evaluation of Carboxy Analogues of Arginine Methyltransferase Inhibitor 1 (AMI‐1)
Authors: Castellano Sabrina , Milite Ciro , Ragno Rino , Simeoni Silvia , Mai Antonello , Limongelli Vittorio , Novellino Ettore , Bauer Ingo , Brosch Gerald , Spannhoff Astrid , Cheng Donghang , Bedford Mark T. , Sbardella Gianluca 
Published: January 2010

AbstractHere we report the synthesis of a number of compounds structurally related to arginine methyltransferase inhibitor 1 (AMI‐1). The structural alterations that we made included: 1) the substitution of the sulfonic groups with the bioisosteric carboxylic groups; 2) the replacement of the ureidic function with a bis‐amidic moiety; 3) the introduction of a N‐containing basic moiety; and 4) the positional isomerization of the aminohydroxynaphthoic moiety. We have assessed the biological activity of these compounds against a panel of arginine methyltransferases (fungal RmtA, hPRMT1, hCARM1, hPRMT3, hPRMT6) and a lysine methyltransferase (SET7/9) using histone and nonhistone proteins as substrates. Molecular modeling studies for a deep binding‐mode analysis of test compounds were also performed. The bis‐carboxylic acid derivatives1 band7 bemerged as the most effective PRMT inhibitors, both in vitro and in vivo, being comparable or even better than the reference compound (AMI‐1) and practically inactive against the lysine methyltransferase SET7/9.
Identifying the efferent projections of leptin‐responsive neurons in the dorsomedial hypothalamus using a novel conditional tracing approach
Authors: Gautron Laurent , Lazarus Michael , Scott Michael M. , Saper Clifford B. , Elmquist Joel K. 
Published: January 2010

AbstractTracing the axonal projections of selected neurons is labor intensive and inherently limited by currently available neuroanatomical methods. We developed an adeno‐associated virus (AAV) that can be used for efficiently tracing identified neuronal populations. The virus encodes a humanized Renilla green fluorescent protein (hrGFP) that is transcriptionally silenced by a neo cassette flanked by LoxH/LoxP sites (AAV‐lox‐Stop‐hrGFP). Thus, hrGFP is expressed only in neurons with Cre recombinase activity. To demonstrate the utility of this approach, the virus was injected unilaterally into the dorsomedial hypothalamus (DMH) of mice that express Cre in neurons expressing the leptin receptor. Animals with DMH injections showed robust hrGFP expression in DMH neurons, as visualized by its endogenous fluorescence or following immunolabeling. We found that hrGFP was expressed in approximately one‐third to one‐half of Cre‐expressing neurons at the site of injection, but not in non‐Cre‐expressing neurons. The expression of GFP allowed us to identify the projection fields of DMH leptin‐responsive neurons. Our results show hrGFP‐positive axonal projections and terminals in the paraventricular nucleus of the hypothalamus, arcuate nucleus, preoptic area, bed nucleus of the stria terminalis, paraventricular thalamus, periaqueductal gray, and precoeruleus. The aforementioned pattern of projections was similar to DMH projections determined by injections of biotinylated dextran amine in the mouse DMH. Interestingly, some hrGFP‐positive terminals were seen contacting the ependymal layer of the third and fourth ventricles. In summary, this approach is an effective tool for tracing axonal projections of chemically identified neurons, including leptin‐responsive neurons. J. Comp. Neurol. 518:2090–2108, 2010. © 2010 Wiley‐Liss, Inc.
Connections of the auditory brainstem in a songbird, Taeniopygia guttata. III. Projections of the superior olive and lateral lemniscal nuclei
Authors: Wild J. Martin , Krützfeldt Nils O.E. , Kubke M. Fabiana 
Published: January 2010

AbstractSequential to companion articles that report the projections of the cochlear nucleus angularis (NA) and the third‐order nucleus laminaris (NL) to the central nucleus of the inferior colliculus (MLd) and to the superior olive (OS) and lateral lemniscal nuclei (LLV, LLI, and LLD) (Krützfeldt et al., J Comp Neurol, this issue), we here describe the projections of the latter group of nuclei using standard tract‐tracing methods. OS projects on LLV and both have further ascending projections on LLI, LLD, and MLd. LLV also provides auditory input to the song system, via nucleus uvaeformis, and to the thalamo‐telencephalic auditory system, via nucleus ovoidalis (Ov), thus bypassing MLd. The two divisions of LLD (LLDa and LLDp) project across the midline via the commissure of Probst each to innervate the homologous contralateral nucleus and MLd. Both, particularly LLDp, also project on Ov. Injections in LLD and LLV resulted in anterograde labeling of caudal nucleus basorostralis (Bas) in the frontal telencephalon, but retrograde tracing so far suggests that only LLI is a real source of this projection (Wild and Farabaugh [1996] J Comp Neurol 365:306–328). OS and LLV also have descending projections on the ipsilateral NA, NM, and NL, and LLV also projects on OS. The ascending inputs to MLd and more rostral nuclei may contribute importantly to mechanisms of auditory pattern (song) recognition. Consistent with previous studies, some of the descending projections may be inhibitory. J. Comp. Neurol. 518:2149–2167, 2010. © 2010 Wiley‐Liss, Inc.
Sex‐specific antennal sensory system in the ant Camponotus japonicus: Glomerular organizations of antennal lobes
Authors: Nakanishi Aki , Nishino Hiroshi , Watanabe Hidehiro , Yokohari Fumio , Nishikawa Michiko 
Published: January 2010

AbstractAnts have well‐developed chemosensory systems for social lives. The goal of our study is to understand the functional organization of the ant chemosensory system based on caste‐ and sex‐specific differences. Here we describe the common and sex‐specific glomerular organizations in the primary olfactory center, the antennal lobe of the carpenter ant Camponotus japonicus. Differential labeling of the two antennal nerves revealed distinct glomerular clusters innervated by seven sensory tracts (T1–T7 from ventral to dorsal) in the antennal lobe. T7 innervated 10 glomeruli, nine of which received thick axon terminals almost exclusively from the ventral antennal nerve. Coelocapitular (hygro‐/thermoreceptive), coeloconic (thermoreceptive), and ampullaceal (CO2‐receptive) sensilla, closely appositioned in the flagellum, housed one or three large sensory neurons supplying thick axons exclusively to the ventral antennal nerve. These axons, therefore, were thought to project into T7 glomeruli in all three castes. Workers and virgin females had about 140 T6 glomeruli, whereas males completely lacked these glomeruli. Female‐specific basiconic sensilla (cuticular hydrocarbon‐receptive) contained over 130 sensory neurons and were completely lacking in males' antennae. These sensory neurons may project into T6 glomeruli in the antennal lobe of workers and virgin females. Serotonin‐immunopositive neurons innervated T1–T5 and T7 glomeruli but not T6 glomeruli in workers and virgin females. Because males had no equivalents to T6 glomeruli, serotonin‐immunopositive neurons appeared to innervate all glomeruli in the male's antennal lobe. T6 glomeruli in workers and virgin females are therefore female‐specific and may have functions related to female‐specific tasks in the colony rather than sexual behaviors. J. Comp. Neurol. 518:2186–2201, 2010. © 2010 Wiley‐Liss, Inc.
Postnatal development of GABA‐immunoreactive neurons and terminals in rat periaqueductal gray matter: A light and electron microscopic study
Authors: Barbaresi Paolo 
Published: January 2010

AbstractThe development of intrinsic gamma‐aminobutyric acid (GABA)‐ergic neurons was studied in the first month of postnatal life in the rat periaqueductal gray matter (PAG) by light and electron microscopy using an anti‐GABA serum. At birth (postnatal day 0: P0) GABA‐immunopositive (GABAIP) neurons were detected only on the outer edge of dorsolateral PAG (PAG‐DL) and were rare in the other PAG subdivisions. Their distribution did not change from P0 to P5, while they increased progressively from P5 to P10 in PAG‐DL and began to be detected in ventrolateral PAG (PAG‐VL). At the end of the second postnatal week the immunostaining pattern was nearly adult‐like, and between P20 and P30 the adult pattern of GABA immunoreactivity was established. Quantitative light microscopic examination indicated that in the first postnatal month the cross‐sectional area of GABAIP neurons gradually increased from 67.63 and 78.69 μm2 at P0 to 122.15 and 119.16 μm2 at P30 in PAG‐DL and PAG‐VL, respectively. Electron microscopic observations disclosed GABA labeling from P0 in cell bodies, dendrites, growth cones, and axon terminals. GABAIP terminals were few in neonatal rats and became more numerous and morphologically mature around the second week. Synapse development and maturation were examined by quantitative ultrastructural analysis. Synaptic vesicle number and size of GABAIP axon terminals progressively grew in the first postnatal month. In conclusion, the number and size of GABAIP cells progressively increase in postnatal PAG, with two populations of intrinsic neurons expressing their GABAergic nature in two different periods. J. Comp. Neurol. 518:2240–2260, 2010. © 2010 Wiley‐Liss, Inc.
Cuprizone‐induced demyelination in CNP::GFP transgenic mice
Authors: Silvestroff Lucas , Bartucci Sandra , Soto Eduardo , Gallo Vittorio , Pasquini Juana , Franco Paula 
Published: January 2010

AbstractCuprizone (bis‐cyclohexanone oxaldihydrazone) was previously shown to induce demyelination in white matter enriched brain structures. In the present study we used the cuprizone demyelination model in transgenic mice expressing the enhanced green fluorescent protein (GFP) under the 2′‐3′‐cyclic nucleotide 3′‐phosphodiesterase (CNPase) promoter. The use of these particular transgenic mice allows easy detection of cells belonging to the entire oligodendroglial (OLG) lineage, ranging from OLG precursors to mature myelinating OLGs. We were able to evaluate the precise extent of oligodendroglial cell damage and recovery within the murine adult central nervous system (CNS) after inducing demyelination by acute cuprizone intoxication. A generalized loss of GFP+ cells was observed after cuprizone exposure and correlated with a decline in myelin basic protein (MBP) expression. OLGs were depleted in many brain areas that were previously thought to be unaffected by cuprizone treatment. Thus, in addition to the well‐known cuprizone effects on the medial corpus callosum, we also found a loss of GFP+ cells in most brain structures, particularly in the caudatus putamen, cortex, anterior commissure, olfactory bulb, hippocampus, optic chiasm, brainstem, and cingulum. Loss of GFP+ cells was accompanied by extensive astrogliosis and microglial activation, although neurons were not affected. Interestingly, cuprizone‐treated animals showed both activation of GFAP expression and a higher proliferation rate in subventricular zone cells. A week after cuprizone removal from the diet, GFP+ oligodendroglial cells began repopulating the damaged structures. GFP expression precedes that of MBP and allows OLG detection before myelin restoration. J. Comp. Neurol. 518:2261–2283, 2010. © 2010 Wiley‐Liss, Inc.
Parallel but separate inputs from limbic cortices to the mammillary bodies and anterior thalamic nuclei in the rat
Authors: Wright Nicholas F. , Erichsen Jonathan T. , Vann Seralynne D. , O'Mara Shane M. , Aggleton John P. 
Published: January 2010

AbstractThe proposal that separate populations of subicular cells provide the direct hippocampal projections to the mammillary bodies and anterior thalamic nuclei was tested by placing two different fluorescent tracers in these two sites. In spite of varying the injection locations within the mammillary bodies and within the three principal anterior thalamic nuclei and the lateral dorsal thalamic nucleus, the overall pattern of results remained consistent. Neurons projecting to the thalamus were localized to the deepest cell populations within the subiculum while neurons projecting to the mammillary bodies consisted of more superficially placed pyramidal cells within the subiculum. Even when these two cell populations become more intermingled, e.g., in parts of the intermediate subiculum, almost no individual cells were found to project to both diencephalic targets. In adjacent limbic areas, i.e., the retrosplenial cortex, postsubiculum, and entorhinal cortex, populations of cells that project to the anterior thalamic nuclei and mammillary bodies were completely segregated. This segregated pattern included afferents to those nuclei comprising the head‐direction system. The sole exception was a handful of double‐labeled cells, mainly confined to the ventral subiculum, that were only found after pairs of injections in the anteromedial thalamic nucleus and mammillary bodies. The projections to the anterior thalamic nuclei also had a septal‐temporal gradient with relatively fewer cells projecting from the ventral (temporal) subiculum. These limbic projections to the mammillary bodies and anterior thalamus comprise a circuit that is vital for memory, within which the two major components could convey parallel, independent information. J. Comp. Neurol. 518:2334–2354, 2010. © 2010 Wiley‐Liss, Inc.
Visual deprivation increases accumulation of dense core vesicles in developing optic tectal synapses in Xenopus laevis
Authors: Li Jianli , Cline Hollis T. 
Published: January 2010

AbstractDespite considerable progress in understanding the molecular components of synapses in the central nervous system, the ultrastructural rearrangements underlying synaptic development remain unclear. We used serial section transmission electron microscopy and three‐dimensional reconstructions of the optic tectal neuropil of Xenopus laevis tadpoles to detect and quantify changes in synaptic ultrastructure over a 1‐week period from stages 39 and 47, during which time the visual system of Xenopus tadpoles becomes functional. Synapse density, presynaptic maturation index, and number of synapses per axon bouton increase, whereas the number of DCVs per bouton decreases, between stages 39 and 47. The width of the synaptic cleft decreased and the diameter of postsynaptic profiles increased between stages 39 and 47 and then remained relatively unchanged after stage 47. We found no significant difference in synapse maturation between GABAergic and non‐GABAergic synapses. To test the effect of visual experience on synaptogenesis, animals were deprived of visual experience for 3 days from stage 42 to 47. Visual deprivation decreased synapse maturation and the number of connections per bouton. Furthermore, visual deprivation increased the number of DCVs per bouton by more than twofold. The visual‐deprivation‐induced decrease in synaptic connections is specific to asymmetric non‐GABAergic synapses; however, both symmetric GABAergic and asymmetric synapses show comparable increases in the number DCVs with visual deprivation. In both the control and the visually deprived animals, the number of DCVs per bouton is highly variable and does not correlate with either synapse maturation or the number of connected partners per bouton. These data suggest that synaptogenesis and DCV accumulation are regulated by visual experience and further suggest a complex spatial and temporal relation between DCV accumulation and synapse formation. J. Comp. Neurol. 518:2365–2381, 2010. © 2010 Wiley‐Liss, Inc.
Neonatal androgen‐dependent sex differences in lumbar spinal cord dopamine concentrations and the number of A11diencephalospinal dopamine neurons
Authors: Pappas Samuel S. , Tiernan Chelsea T. , Behrouz Bahareh , Jordan Cynthia L. , Breedlove S. Marc , Goudreau John L. , Lookingland Keith J. 
Published: January 2010

AbstractA11diencephalospinal dopamine (DA) neurons provide the major source of DA innervation to the spinal cord. DA in the dorsal and ventral horns modulates sensory, motor, nociceptive, and sexual functions. Previous studies from our laboratory revealed a sex difference in the density of DA innervation in the lumbar spinal cord. The purpose of this study was to determine whether sex differences in spinal cord DA are androgen dependent, influenced by adult or perinatal androgens, and whether a sex difference in the number of lumbar‐projecting A11neurons exists. Adult male mice have significantly higher DA concentrations in the lumbar spinal cord than either females or males carrying the testicular feminization mutation (tfm) in the androgen receptor (AR) gene, suggesting an AR‐dependent origin. Spinal cord DA concentrations are not changed following orchidectomy in adult male mice or testosterone administration to ovariectomized adult female mice. Administration of exogenous testosterone to postnatal day 2 female mice results in DA concentrations in the adult lumbar spinal cord comparable to those of males. Male mice display significantly more lumbar‐projecting A11DA neurons than females, particularly in the caudal portion of the A11cell body region, as determined by retrograde tract tracing and immunohistochemistry directed toward tyrosine hydroxylase. These results reveal an AR‐dependent sex difference in both the number of lumbar‐projecting A11DA neurons and the lumbar spinal cord DA concentrations, organized by the presence of androgens early in life. The AR‐dependent sex difference suggests thyat this system serves a sexually dimorphic function in the lumbar spinal cord. J. Comp. Neurol. 518:2423–2436, 2010. © 2010 Wiley‐Liss, Inc.
Behavior of Two Almost Identical Spins during the CPMG Pulse Sequence
Authors: Tošner Zdeněk , Škoch Antonín , Kowalewski Jozef 
Published: January 2010

AbstractMultiple‐spin‐echo experiments have found wide use in nuclear magnetic resonance spectroscopy. In particular, the Carr–Purcell–Meiboom–Gill (CPMG) pulse sequence is used to determine transverse relaxation times T2. Herein it is demonstrated, both theoretically and experimentally, that for a pair of almost identical spins‐1/2 the experimental setup can have a profound effect on the observed spin dynamics. It is shown that, in the case of dipolar relaxation, the measured T2 values can roughly vary between the limits of identical and unlike spins, just depending on the repetition rate of π pulses with respect to chemical shift separation. Such an effect can, in the extreme narrowing regime, amount to a 50 % difference.