bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of the Five RNA Helicases in the Adaptive Response of Bacillus cereus ATCC 14579 Cells to Temperature, pH, and Oxidative Stresses
Authors: Pandiani Franck , Chamot Stéphanie , Brillard Julien , Carlin Frédéric , Nguyen-the Christophe , Broussolle Véronique 
Published: June 2011

ABSTRACT

In this study, growth rates and lag times of the five RNA helicase-deleted mutants of
Bacillus cereus
ATCC 14579 were compared to those of the wild-type strain under thermal, oxidative, and pH stresses. Deletion of
cshD
and
cshE
had no impact under any of the tested conditions. Deletion of
cshA
,
cshB
, and
cshC
abolished growth at 12°C, confirming previous results. In addition, we found that each RNA helicase had a role in a specific temperature range: deletion of
cshA
reduced growth at all the tested temperatures up to 45°C, deletion of
cshB
had impact below 30°C and over 37°C, and deletion of
cshC
led mainly to a cold-sensitive phenotype. Under oxidative conditions, deletion of
cshB
and
cshC
reduced growth rate and increased lag time, while deletion of
cshA
increased lag time only with H
2
O
2
and reduced growth rate at a high diamide concentration. Growth of the Δ
cshA
strain was affected at a basic pH independently of the temperature, while these conditions had a limited effect on
ΔcshB
and
ΔcshC
strain growth. The RNA helicases CshA, CshB, and CshC could participate in a general adaptation pathway to stressful conditions, with a stronger impact at low temperature and a wider role of CshA.
Annotated Genome Sequence of Lactobacillus pentosusMP-10, Which Has Probiotic Potential, from Naturally Fermented Aloreña Green Table Olives
Authors: Abriouel Hikmate , Benomar Nabil , Pérez Pulido Rubén , Cañamero Magdalena Martínez , Gálvez Antonio 
Published: June 2011

ABSTRACT

Lactobacillus pentosus
MP-10 was isolated from brines of naturally fermented Aloreña green table olives. MP-10 has potential probiotic traits, including inhibition of human pathogenic bacteria, survival at low pH (1.5), and bile salt tolerance (3%). Here, we report for the first time the annotated genome sequence of
L. pentosus
.
Complete Genome Sequence of the Obligate Piezophilic Hyperthermophilic Archaeon Pyrococcus yayanosii CH1
Authors: Jun Xu , Lupeng Liu , Minjuan Xu , Oger Philippe , Fengping Wang , Jebbar Mohamed , Xiang Xiao 
Published: June 2011

ABSTRACT

Pyrococcus yayanosii
CH1 is the first obligate piezophilic hyperthermophilic archaeon isolated from the deep-sea hydrothermal site Ashadze on the mid-Atlantic ridge at a depth of 4,100 m. This organism grows within a temperature range of 80 to 108°C and a hydrostatic pressure range of 20 to 120 MPa, with optima at 98°C and 52 MPa, respectively. Here, we report the complete genome sequence (1,716,817 bp, with a G+C content of 51.6%) of the type strain
P. yayanosii
CH1
T
(= JCM 16557). This genomic information reveals a systematic view of the piezoadaptation strategy and evolution scenario of metabolic pathways in
Thermococcales
.
Draft Genome of the Psychrotolerant Acidophile Acidithiobacillus ferrivorans SS3
Authors: Liljeqvist Maria , Valdes Jorge , Holmes David S. , Dopson Mark 
Published: June 2011

ABSTRACT

Acidithiobacillus ferrivorans
SS3 is a psychrotolerant acidophile capable of growth in the range of 5° to 30°C (optimum, ≈25°C). It gains energy from the oxidation of ferrous iron and inorganic sulfur compounds and obtains organic carbon from carbon dioxide. Here, we present the draft genome sequence of
A. ferrivorans
SS3 that will permit investigation of genes involved in growth in acidic environments at low temperatures.
Draft Genome Sequences of Two Pseudomonas aeruginosa Clinical Isolates with Different Antibiotic Susceptibilities
Authors: Soares-Castro P. , Marques D. , Demyanchuk S. , Faustino A. , Santos P. M. 
Published: June 2011

ABSTRACT

Pseudomonas aeruginosa
is a primary cause of opportunistic infections. We have sequenced and annotated the genomes of two
P. aeruginosa
clinical isolates evidencing different antibiotic susceptibilities. Registered differences in the composition of their accessory genomes may provide clues on
P. aeruginosa
strategies to thrive in different environments like infection loci.
Whole-Genome Shotgun Sequencing of an Indian-Origin Lactobacillus helveticus Strain, MTCC 5463, with Probiotic Potential
Authors: Prajapati J. B. , Khedkar C. D. , Chitra J. , Suja Senan , Mishra V. , Sreeja V. , Patel R. K. , Ahir V. B. , Bhatt V. D. , Sajnani M. R. , Jakhesara S. J. , Koringa P. G. , Joshi C. G. 
Published: June 2011

ABSTRACT

Lactobacillus helveticus
MTCC 5463 was isolated from a vaginal swab from a healthy adult female. The strain exhibited potential probiotic properties, with their beneficial role in the gastrointestinal tract and their ability to reduce cholesterol and stimulate immunity. We sequenced the whole genome and compared it with the published genome sequence of
Lactobacillus helveticus
DPC4571.
Genome Sequence of Haloplasma contractile, an Unusual Contractile Bacterium from a Deep-Sea Anoxic Brine Lake
Authors: Antunes André , Alam Intikhab , El Dorry Hamza , Siam Rania , Robertson Anthony , Bajic Vladimir B. , Stingl Ulrich 
Published: June 2011

ABSTRACT

We present the draft genome of
Haloplasma contractile
, isolated from a deep-sea brine and representing a new order between
Firmicutes
and
Mollicutes
. Its complex morphology with contractile protrusions might be strongly influenced by the presence of seven MreB/Mbl homologs, which appears to be the highest copy number ever reported.
Complete Genome Sequence of the Marine Cellulose- and Xylan-Degrading Bacterium Glaciecolasp. Strain 4H-3-7+YE-5
Authors: Klippel Barbara , Lochner Adriane , Bruce David C. , Walston Davenport Karen , Detter Chris , Goodwin Lynne A. , Han James , Han Shunsheng , Land Miriam L. , Mikhailova Natalia , Nolan Matt , Pennacchio Len , Pitluck Sam , Tapia Roxanne , Woyke Tanja , Wiebusch Sigrid , Basner Alexander , Abe Fumiyoshi , Horikoshi Koki , Keller Martin , Antranikian Garabed 
Published: June 2011

ABSTRACT

Glaciecola
sp. strain 4H-3-7+YE-5 was isolated from subseafloor sediments at Suruga Bay in Japan and is capable of efficiently hydrolyzing cellulose and xylan. The complete genome sequence of
Glaciecola
sp. 4H-3-7+YE-5 revealed several genes encoding putatively novel glycoside hydrolases, offering a high potential for plant biomass degradation.
Compliance with return-to-play regulations following concussion in Australian schoolboy and community rugby union players
Authors: Hollis Stephanie J , Stevenson Mark R , McIntosh Andrew S , Shores E Arthur , Finch Caroline F 
Published: June 2011

BackgroundThere is a risk of concussion when playing rugby union. Appropriate management of concussion includes compliance with the return-to-play regulations of the sports body for reducing the likelihood of premature return-to-play by injured players.PurposeTo describe the proportion of rugby union players who comply with the sports body's regulations on returning to play postconcussion.Study designProspective cohort study.Methods1958 community rugby union players (aged 15–48 years) in Sydney (Australia) were recruited from schoolboy, grade and suburban competitions and followed over ≥1 playing seasons. Club doctors/physiotherapists/coaches or trained injury recorders who attended the game reported players who sustained a concussion. Concussed players were followed up over a 3-month period and the dates when they returned to play (including either a game or training session) were recorded, as well as any return-to-play advice they received.Results187 players sustained ≥1 concussion throughout the follow-up. The median number of days before players returned to play (competition game play or training) following concussion was 3 (range 1–84). Most players (78%) did not receive return-to-play advice postconcussion, and of those who received correct advice, all failed to comply with the 3-week stand-down regulation.ConclusionsThe paucity of return-to-play advice received by community rugby union players postconcussion and the high level of non-compliance with return-to-play regulations highlight the need for better dissemination and implementation of the return-to-play regulations and improved understanding of the underlying causes of why players do not adhere to return-to-play practices.
Mortality among Swedish chimney sweeps (1952–2006): an extended cohort study
Authors: Jansson Catarina , Alderling Magnus , Hogstedt Christer , Gustavsson Per 
Published: June 2011

ObjectivesWe extended a cohort study of Swedish chimney sweeps and prolonged follow-up in order to increase power and study those first employed after 1950 when oil began to replace wood as a main fuel for heating in Sweden.MethodsMale Swedish chimney sweeps who were members of the national trade union in 1981–2006 were identified (n=1087) and included to a previous cohort of those employed in 1918–1980 (n=5287). All employment histories were updated, and the total extended cohort (n=6374) was linked to the registers of Causes of Death and Total Population and followed for mortality from 1952 through 2006. Standardised mortality ratios (SMRs) were estimated using the Swedish male population as reference.Results1841 observed deaths resulted in an SMR for all causes of deaths of 1.29 (95% CI 1.24 to 1.36). Mortality was significantly increased for all malignant tumours, oesophageal cancer, bowel cancer, liver cancer, lung cancer, alcoholism, ischaemic heart disease, non-malignant respiratory diseases, liver cirrhosis, external causes and suicides. The lung cancer SMR remained increased, although attenuated, after adjustment for group-level smoking data, SMR of 1.52 (95% CI 1.26 to 1.89). Duration of employment showed no consistent evidence of dose–response associations. Alcohol-related deaths (liver cirrhosis and alcoholism) were not increased among those employed >30 years. Mortality among those employed after 1950 was similar to that of the entire cohort.ConclusionsChimney sweeps are exposed to high levels of toxic substances in the occupation, but excess alcohol and smoking habits were also observed, and the results must be interpreted cautiously. However, group-level data on tobacco smoking indicated that the lung cancer excess only to some extent could be explained by smoking habits, and the increased mortality from oesophageal cancer and ischaemic heart disease among chimney sweeps employed >30 years is less likely to be caused by excess alcohol habits.
Republished review: Ocular manifestations of the antiphospholipid syndrome
Authors: Utz Virginia Miraldi , Tang Johnny 
Published: June 2011

ABSTRACT
Antiphospholipid syndrome (APS) is an autoimmune disease characterised by a heterogenous group of antibodies directed against negatively charged phospholipids including antiphospholipid antibodies (aPL), anticardiolipin antibodies (aCL) and β-2 glycoprotein I (aβ-2-GP1). The major features of this disorder include arterial and venous thrombosis and recurrent fetal loss. The vasculature of the eye is frequently involved and may be the presenting manifestation. A diagnosis of APS should be considered in a young patients without traditional thromboembolic risk factors presenting with ocular vaso-occlusive disease. Management of these patients involves a team-approach with a haematologist/oncologist or rheumatologist to manage the coagulation status of these patients to prevent further systemic vascular occlusions.
Older partner selection, sexual risk behaviour and unrecognised HIV infection among black and Latino men who have sex with men
Authors: Joseph Heather A , Marks Gary , Belcher Lisa , Millett Gregorio A , Stueve Ann , Bingham Trista A , Lauby Jennifer 
Published: June 2011

ObjectivesThe authors examine whether young black and Latino men who have sex with men (MSM) who have older sex partners are more likely than those who do not have older sex partners to have unrecognised HIV infection. The authors examine whether the association stems from (1) increased sexual risk behaviour with male partners of any age, (2) heightened risk of being exposed to HIV infection by older partners or (3) a combination of these two factors.MethodsThe analytical sample consisted of 723 black and Latino MSM, aged 18–35 years, who were HIV negative or of unknown serostatus at study entry. Participants completed a self-administered questionnaire and were tested for HIV infection. Men who reported having a male sex partner who was at least 4 years older than themselves were compared with those who did not. Outcomes included unprotected receptive anal intercourse (URAI) with male partners of any age (past 3 months) and having unrecognised HIV infection.ResultsMen with older partners reported a higher prevalence of URAI (AOR=1.50, 95% CI 1.02 to 2.21). A second model found that men with older partners had increased odds of having unrecognised HIV infection (AOR=2.51, 95% CI 1.18 to 5.34) after controlling for the number of URAI partners of any age, which remained an independent predictor.ConclusionsYoung black and Latino MSM who had older male sex partners were at increased risk of having unrecognised HIV infection. This heightened risk was associated with sexual risk behaviour with partners of any age as well as possible increased exposure to HIV infection from older partners.
Potential Prostate Cancer Drug Target: Bioactivation of Androstanediol by Conversion to Dihydrotestosterone
Authors: Mohler James L. , Titus Mark A. , Wilson Elizabeth M. 
Published: June 2011

AbstractHigh-affinity binding of dihydrotestosterone (DHT) to the androgen receptor (AR) initiates androgen-dependent gene activation, required for normal male sex development in utero, and contributes to prostate cancer development and progression in men. Under normal physiologic conditions, DHT is synthesized predominantly by 5α-reduction of testosterone, the major circulating androgen produced by the testis. During androgen deprivation therapy, intratumoral androgen production is sufficient for AR activation and prostate cancer growth, even though circulating testicular androgen levels are low. Recent studies indicate that the metabolism of 5α-androstane-3α, 17β-diol by 17β-hydroxysteroid dehydrogenase 6 in benign prostate and prostate cancer cells is a major biosynthetic pathway for intratumoral synthesis of DHT, which binds AR and initiates transactivation to promote prostate cancer growth during androgen deprivation therapy. Drugs that target the so-called backdoor pathway of DHT synthesis provide an opportunity to enhance clinical response to luteinizing-hormone–releasing hormone (LHRH) agonists or antagonists, AR antagonists, and inhibitors of 5α-reductase enzymes (finasteride or dutasteride), and other steroid metabolism enzyme inhibitors (ketoconazole or the recently available abiraterone acetate). Clin Cancer Res; 17(18); 5844–9. ©2011 AACR.
Resveratrol Selectively Induces DNA Damage, Independent of Smad4 Expression, in Its Efficacy against Human Head and Neck Squamous Cell Carcinoma
Authors: Tyagi Alpna , Gu Mallikarjuna , Takahata Takenori , Frederick Barbara , Agarwal Chapla , Siriwardana Sunitha , Agarwal Rajesh , Sclafani Robert A. 
Published: June 2011

Abstract
Purpose: Alterations in Smad4 signaling and its loss cause genomic instability and head and neck squamous cell carcinoma (HNSCC), suggesting that agents that target both Smad4-dependent and -independent pathways could control HNSCC.
Experimental Design: Resveratrol efficacy was evaluated against the HNSCC cells FaDu, Cal27, Det562, and Cal27-Smad4 for viability, DNA damage, cell-cycle progression, and apoptosis, as well as γ-H2AX expression, and focus formation (γ-H2AX and Brca1). Resveratrol efficacy was also examined in nude mice for FaDu xenograft growth. Xenografts were analyzed for γ-H2AX and cleaved caspase-3.
Results: Resveratrol (5–50 μmol/L) suppressed viability and induced DNA damage in FaDu and Cal27 cells but not in normal human epidermal keratinocytes and human foreskin fibroblasts, showing its selectivity toward HNSCC cells; however, Det562 cells were resistant to resveratrol even at 100 μmol/L. Cal27 cells stably transfected with Smad4 showed similar resveratrol effects as parental Cal27, indicating that a lack of resveratrol effect in Det562 cells was independent of Smad4 status in these cells. Furthermore, resveratrol caused S-phase arrest and apoptotic death of FaDu and Cal27 cells together with induction of Brca1 and γ-H2AX foci. Resveratrol (50 mg/kg body weight) treatment also inhibited FaDu tumor growth in nude mice, and γ-H2AX and cleaved caspase-3 were strongly increased in xenografts from resveratrol-treated mice compared with controls.
Conclusion: Our findings for the first time showed antiproliferative, DNA damaging, and apoptotic effects of resveratrol in HNSCC cells independent of Smad4 status, both in vitro and in vivo, suggesting that more studies are needed to establish its potential usefulness against HNSCC. Clin Cancer Res; 17(16); 5402–11. ©2011 AACR.
Delayed Paraplegia After Spinal Cord Ischemic Injury Requires Caspase-3 Activation in Mice
Authors: Kakinohana Manabu , Kida Kotaro , Minamishima Shizuka , Atochin Dmitriy N. , Huang Paul L. , Kaneki Masao , Ichinose Fumito 
Published: June 2011

Background and Purpose—
Delayed paraplegia remains a devastating complication after ischemic spinal cord injury associated with aortic surgery and trauma. Although apoptosis has been implicated in the pathogenesis of delayed neurodegeneration, mechanisms responsible for the delayed paraplegia remain incompletely understood. The aim of this study was to elucidate the role of apoptosis in delayed motor neuron degeneration after spinal cord ischemia.

Methods—
Mice were subjected to spinal cord ischemia induced by occlusion of the aortic arch and left subclavian artery for 5 or 9 minutes. Motor function in the hind limb was evaluated up to 72 hours after spinal cord ischemia. Histological studies were performed to detect caspase-3 activation, glial activation, and motor neuron survival in the serial spinal cord sections. To investigate the impact of caspase-3 activation on spinal cord ischemia, outcome of the spinal cord ischemia was examined in mice deficient for caspase-3.

Results—
In wild-type mice, 9 minutes of spinal cord ischemia caused immediate paraplegia, whereas 5 minutes of ischemia caused delayed paraplegia. Delayed paraplegia after 5 minutes of spinal cord ischemia was associated with histological evidence of caspase-3 activation, reactive astrogliosis, microglial activation, and motor neuron loss starting at approximately 24 to 48 hours after spinal cord ischemia. Caspase-3 deficiency prevented delayed paraplegia and motor neuron loss after 5 minutes of spinal cord ischemia, but not immediate paraplegia after 9 minutes of ischemia.

Conclusions—
The present results suggest that caspase-3 activation is required for delayed paraplegia and motor neuron degeneration after spinal cord ischemia.
A Systematic Review of Stenting and Angioplasty of Symptomatic Extracranial Vertebral Artery Stenosis
Authors: Stayman Aaron N. , Nogueira Raul G. , Gupta Rishi 
Published: June 2011

Background and Purpose—
Extracranial vertebral artery stenosis (ECVAS) is common among patients with ischemic stroke. Despite the limited knowledge of the natural history of patients with symptomatic vertebral disease, endovascular revascularization techniques are now utilized in clinical practice. We sought to determine the risk of endovascular treatment for ECVAS with a systematic review of the literature.

Methods—
A search strategy was used using the terms “stenting,” “vertebral,” “ostium,” “origin,” and “extracranial” through Medline. All articles were reviewed along with their references to determine the risk and durability of endovascular treatment.

Results—
A total of 27 articles were identified that met inclusion criterion, with a total of 980 of 993 patients treated with stents. The majority of patients (56%) were noted to have contralateral vertebral artery stenosis or occlusion and 92% were symptomatic at the time of treatment. A total of 11 patients (1.1%) experienced a stroke and 8 (0.8%) experienced a transient ischemic attack within 30 days of the procedure. Drug-eluting stents were associated with lower restenosis rates (11%) compared to bare metal stents (30%) at a mean of 24 months of follow-up.

Conclusions—
Stenting and angioplasty of ECVAS appear to have a low rate of periprocedural stroke or transient ischemic attack and restenosis rates that may not be as high as suspected. Given the frequency of ECVAS as an etiology for ischemic stroke, future studies aimed at determining efficacy of this treatment modality relative to medical therapy would be of benefit to clinicians caring for these patients.
Oxidative Damage in Ischemic Stroke Revealed Using Multiple Biomarkers
Authors: Seet Raymond C.S. , Lee Chung-Yung J. , Chan Bernard P.L. , Sharma Vijay K. , Teoh Hock-Luen , Venketasubramanian Narayanaswamy , Lim Erle C.H. , Chong Wan-Ling , Looi Woan-Foon , Huang Shan-Hong , Ong Benjamin K.C. , Halliwell Barry 
Published: June 2011

Background and Purpose—
We investigated changes in oxidative damage after ischemic stroke using multiple biomarkers.

Methods—

Serial blood and urine samples of ischemic stroke subjects and age-matched control subjects were assayed for F
2
-isoprostanes, hydroxyeicosatetraenoic acid products, F
4
-neuroprostanes, 24-hydroxycholesterol, allantoin, and urate.

Results—

Sixty-six stroke subjects (mean age, 65 years; median National Institutes of Health Stroke Scale 17) and 132 control subjects were recruited. A bimodal pattern of change was observed in plasma and urinary F
2
-isoprostanes and plasma 24-hydroxycholesterol. The rise in plasma hydroxyeicosatetraenoic acid products, F
4
-neuroprostanes, and allantoin was highest 6 to 12 hours after stroke onset, whereas plasma urate was significantly lower than controls on Days 1 to 3. After adjusting for age and baseline National Institutes of Health Stroke Scale, baseline plasma esterified hydroxyeicosatetraenoic acid products (OR, 1.01; 95% CI, 1.01 to 1.02), plasma urate (1.01; 1.00 to 1.01), and plasma free F
4
-neuroprostanes (2.73; 1.76 to 3.93) were associated with 90-day good functional recovery (modified Rankin Scale ≤1).

Conclusions—
Multiple markers of oxidative damage are increased immediately after stroke and remain elevated for several days. Recognition of these temporal changes may help design better antioxidant treatment trials for acute ischemic stroke.