bluuebunny/crossref_metadata_2025_split huggingface dataset:
Inhibition of CD146 gene expression via RNA interference reduces in vitro perineural invasion on ACC‐M cell
Authors: Chen W. , Zhang H. L. , Jiang Y. G. , Li J. H. , Liu B. L. , Sun M. Y. 
Published: August 2010

Background:  Overexpression of melanoma cell adhesion molecule (MCAM or CD146), a novel member of the immunoglobulin gene superfamily, promotes invasion, metastasis, growth and survival of malignant cells.Materials and methods:  Here, we used a human U6 promoter‐driven DNA template approach to induce short hairpin RNA (shRNA)‐triggered RNA interference to block CD146 gene expression in the human high‐metastatic adenoid cystic carcinoma (ACC) cell line ACC‐M. reverse transcription‐polymerase chain reaction, Western blot, 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyl‐2H‐tetrazolium bromide assay, flow cytometry analysis and in vitro perineural invasion (PNI) assay were used to investigate the effects of CD146 on cell growth, cell cycle distribution and cell PNI activity in ACC‐M cells.Results:  We have demonstrated that different shRNAs can specifically knockdown the transcription of CD146, resulting in the inhibition of cell proliferation, and G0/G1 to S phase arrest in ACC‐M cells. Knockdown of CD146 by shRNA resulted in decrease of the ACC‐M PNI activity in vitro.Conclusion:  These findings suggested that CD146 was an ACC‐related gene and CD146 may play an important role in the carcinogenesis, progression and PNI activity of ACC.
Quantitative analysis of Langerhans’ cells in oral chronic graft‐vs.‐host disease
Authors: Orti‐Raduan Érika Sinara Lenharo , Nunes Adauto José Ferreira , Oliveira Denise Tostes , Lara Vanessa Soares , De Assis Taveira Luís Antônio 
Published: August 2010

Background:  The Langerhans cells (LCs) are scattered throughout the epithelium of skin and mucosa and have been associated with the graft‐vs.‐host disease (GVHD), which is the highest cause of morbidity and mortality in patients who underwent bone marrow transplant (BMT). This study aims at quantifying the LCs in the oral chronic GVHD (cGVHD).Methods:  Microscopic sections from biopsies carried out in the buccal mucosa of 40 patients who underwent allogenic BMT and developed (20) or not (20) oral cGVHD (Groups 1 and 2, respectively) were utilised. For the control group, free surgical margins of 20 biopsies of non‐inflammatory lesions in the buccal mucosa (Group 3) were used. The sections were studied in routine colouration and immunostained for CD1a.Results:  Group 1 (with cGVHD) presented a greater number of Langerhans’ cells/mm2 (50.6 ± 37.2) when compared with the other groups (Group 2, 23.11 ± 19.7; Group 3, 16.6 ± 17.3).Conclusion:  Our results suggest a greater recruitment of LCs in patients transplanted with cGVHD, probably as a result of cytokines secreted by the inflammatory cells.
Oral ulcers in HIV‐positive Peruvian patients: an immunohistochemical and in situ hybridization study
Authors: Delgado W. A. , Almeida O. P. , Vargas P. A. , León J. E. 
Published: August 2010

Background:  This study describes the histopathological, immunohistochemical (IHC) and in situ hybridization (ISH) data of 25 cases of oral ulcers in HIV‐positive patients, with clinical and microscopical features similar to ulcers not otherwise specified (NOS)/necrotizing ulcerative stomatitis (NUS).Methods:  Sex, age and clinical history were obtained from the clinical records. Histological analysis for H&E, Gomori–Grocott and Ziehl–Neelsen stains, IHC analysis to detect infectious agents and to characterize inflammatory cellular infiltrate, and ISH for cytomegalovirus (CMV) and EBER1/2 were performed.Results:  Twenty‐one patients were men and four were women (mean age of 34.6 years). The tongue was preferentially affected. Microscopically, the lesions showed extensive necrosis, leukocytoclasia, vasculitis with luminal fibrin clots and an intense inflammatory cellular infiltrate predominated by CD68+ atypical large cells, normal‐sized and crescent‐shaped nuclei macrophages, interspersed by CD8+ T lymphocytes. Mast cells were also observed in all samples studied. CD4+ T lymphocytes, CD20+ B lymphocytes and VS38c+ plasma cells were practically absent. CMV and EBER1/2 were identified in scarce cells of 3 and 16 of 25 cases respectively.Conclusion:  These results show that CD68+ macrophages, followed by CD8+ T lymphocytes, were the predominant inflammatory cells, indicating they are relevant to the pathogenesis of the ulcers, possibly reflecting an abnormal immune response in the oral mucosa. The clinicopathological and immunoprofile features of the present cases are similar to NOS ulcers/NUS in HIV‐positive patients.
Gene expression analysis by oligonucleotide microarray in oral leukoplakia
Authors: Kuribayashi Yuri , Morita Kei‐ichi , Tomioka Hirofumi , Uekusa Masaru , Ito Daisuke , Omura Ken 
Published: August 2010

Background:  Oral leukoplakias (LP) are the most frequent types of oral pre‐cancerous lesions, but there is no accurate assessment of this malignant transformation or even genetic diagnosis of the oral epithelial dysplasia. We need to identify the new genetic diagnosis system of the epithelial dysplasia.Methods:  Oligonucleotide microarray was used to analyze expression patterns of 29 952 genes in 10 LP patients. We compared the different gene expressions between mild dysplasia cases and severe dysplasia cases.Results:  Ninety‐six genes expressed differentially were selected as candidates for up‐regulated in severe dysplasia. Subsequently, we further selected 16 genes with highest differentially expression. By hierarchical clustering analysis, the 10 cases were divided mild dysplasia from severe dysplasia.Conclusions:  The 16 genes are suggested as biomarker gene sets of efficacy and quickly recognized in the development of oral epithelial dysplasia.
Palliative effect of lafutidine on oral burning sensation
Authors: Toida M. , Kato K. , Makita H. , Long N. K. , Takeda T. , Hatakeyama D. , Yamashita T. , Shibata T. 
Published: August 2010

Background:  Lafutidine is a unique histamine H2‐receptor antagonist (H2RA) that has a sensitizing effect on capsaicin‐sensitive afferent neurons (CSAN). This effect may make lafutidine useful for the treatment of burning mouth syndrome (BMS).Methods:  To evaluate the efficacy and safety of lafutidine in patients with oral burning sensation, a randomized controlled trial was performed. Patients who had been receiving other H2RAs with no sensitizing effect on CSAN were randomly assigned to receive lafutidine 10 mg twice daily for 12 weeks, instead of the previous H2RAs, plus gargling with azulene sulfonate sodium (ASS) (lafutidine group, n = 36) or to continue to receive the previous H2RAs plus ASS gargling (control group, n = 35). The intensity of burning sensation was scored by means of a visual analog scale (VAS).Results:  Thirty‐four patients in the lafutidine group and 30 in the control group completed the study. In the lafutidine group, the rate of improvement in the VAS score as compared with the baseline value was significant after 4, 8, and 12 weeks of treatment (P < 0.05). The improvement rate was consistently higher in the lafutidine group than in the control group; the differences between the groups were significant (P < 0.05) after 4, 8, and 12 weeks of treatment. Only two mild abdominal adverse events occurred in the lafutidine group, but neither required the termination of treatment.Conclusion:  Oral lafutidine is very safe and effective for reducing the intensity of oral burning sensation and may therefore be a viable option for the treatment of BMS.
Keratinocyte growth factor colocalized with perlecan at the site of capsular invasion and vascular involvement in salivary pleomorphic adenomas
Authors: Maruyama Satoshi , Cheng Jun , Yamazaki Manabu , Liu Airu , Saku Takashi 
Published: August 2010

Background:  Capsular invasion is often observed in daily pathologic diagnosis of pleomorphic adenomas, although neither actual information about its occurrence nor molecular mechanisms leading to their invasive activities have been reported. In this study, our aim was to elucidate the mode and the frequency of capsular invasion in this tumor and to characterize the tumor cell arrangement at the site of capsular invasion.Methods:  The mode and frequency of capsular invasion of salivary pleomorphic adenomas were histopathologically examined in 104 surgical specimens of pleomorphic adenoma, and stromal characteristics, and tumor cell arrangements at the sites of capsular invasion were immunohistochemically investigated.Results:  A total of 353 areas with capsular invasive changes were collected from 104 cases. The mode of capsular invasion was classified into two types: type I: intracapsular invasion (247 areas, 70%) and type II: capsular penetration (106 areas, 30%). Myxoid stroma, which was perlecan‐immunopositive (+), was shared by both type I and type II sites, while tumor cell foci containing ductal structures were predominant in type II sites. These foci were composed of KGF+ and FGFR2+ cells. In addition, apparent vascular involvement was recognized in 31 tumors (29.8%).Conclusion:  The results suggest that pleomorphic adenoma cells are able to invade into the capsule and involve blood vessels when they are situated in perlecan‐rich milieu, which accelerate KGF signaling.
Oral health and related quality of life status in patients from UK and Turkey: a comparative study in Behcet’s disease
Authors: Mumcu G. , Niazi S. , Stewart J. , Hagi‐Pavli E. , Gokani B. , Seoudi N. , Ergun T. , Yavuz S. , Stanford M. , Fortune F. , Direskeneli H. 
Published: August 2010

Background:  The aim of this study was to evaluate and compare oral health‐related quality of life (oral QoL) in patients from UK and Turkey with Behcet’s disease (BD).Methods:  Thirty‐one BD patients from UK (F/M: 18/13, mean age: 41.8 ± 11.5 years) and Turkey (F/M: 18/13, mean age: 41.5 ± 10.3) who were matched according to age and gender were included in the study. All patients had active oral ulcers. Oral QoL was assessed by Oral Health Impact Profile‐14 (OHIP‐14). Oral health was evaluated by dental and periodontal indices.Results:  No significant difference was found in OHIP‐14 scores between patients from UK (22.7 ± 14.4) and Turkey (20.4 ± 14.3) (P = 0.709). The OHIP‐14 score correlated with the healing time of oral ulcers in UK (r = 0.4, P = 0.04) and the number of oral ulcers in Turkey (r = 0.4, P = 0.012). The number of oral ulcers per month was significantly higher in UK (3.3 ± 2.8) compared with that in Turkey (1.5 ± 2.5) (P = 0.014). However, the number of filled teeth and frequency of tooth brushing were significantly lower in patients from Turkey compared with those in UK (P = 0.000). Similarly, the duration since the last dental visit (5.1 ± 7.2 months) was significantly lower in UK compared with that in Turkey (28.6 ± 23.7 months) (P = 0.000).Conclusions:  Oral QoL was similar in patients from UK and Turkey with active oral ulcers. However, the number of oral ulcers was observed to be higher in UK. As expected, a lower utilization rate of dental services might have led to a poorer oral health in patients from Turkey.
Odontogenic keratocyst expresses vascular endothelial growth factor: an immunohistochemical study
Authors: Mitrou G. K. , Tosios K. I. , Kyroudi A. , Sklavounou A. 
Published: August 2010

Background:  Vascular endothelial growth factor (VEGF) expression may act as a sensitive measure of the angiogenic potential of a lesion. Furthermore, VEGF has been implicated in the pathogenesis of cystic tumors and inflammatory odontogenic cysts. Thus, we studied the expression of VEGF in the epithelium of odontogenic keratocyst (OK) in association with cell proliferation and apoptosis.Methods:  Forty‐two cases of OK, 26 cases of dentigerous cyst (DC), and 15 cases of residual cyst (RC) were retrospectively examined by immunohistochemistry for VEGF, Ki67/Mib‐1 and anti‐caspase‐3. For VEGF and caspase‐3, the intensity of immunostaining was qualitatively assessed, while for the evaluation of Ki67 the average number of positively stained nuclei in 10 high‐power microscopic fields (×400) was calculated.Results:  The VEGF expression was stronger in OK when compared with DC (P < 0.007). The rate of nuclear Ki67 expression in OK was significantly higher than that in DC (P < 0.001) and RC (P < 0.001). Cytoplasmic caspase‐3 expression was statistically more intense in RC than in OK (P = 0.001) or DC (P < 0.001). A statistically significant correlation was seen in OK for Ki67 (P < 0.001) and VEGF (P = 0.023), but not for caspase‐3. Multiple regression analysis revealed a linear relationship between VEGF and Ki67.Conclusions:  The VEGF was expressed in the epithelium of OK, DC, and RC with a variable intensity, and in OK VEGF expression was related to Ki67. It is suggested that VEGF expression by the odontogenic epithelium is not induced solely by inflammation.
Arecoline and oral keratinocytes may affect the collagen metabolism of fibroblasts
Authors: Xia Li , Tian‐You Ling , Yi‐Jun Gao , Dong‐sheng Tang , Wen‐Hui Li 
Published: August 2010

Background:  The characteristic of oral submucous fibrosis (OSF) is related with the disturbance of synthesis and degradation of extracellular matrix. Arecoline, the areca nut (betel nut) component of betel quid, plays a major role in pathogenesis of OSF. But the exact mechanism how arecoline influences the collagen metabolism is unclear.Methods:  Oral keratinocytes and fibroblasts were cocultured and keratinocytes were pre‐treated by arecoline. Fibroblasts alone, fibroblasts stimulated by arecoline, fibroblasts cocultured with keratinocytes and fibroblasts cocultured with keratinocyte pre‐treated by arecoline were included as the four groups in the present study. The concentration of collagen, the content and activity of matrix metalloproteinase (MMP) and the concentration of tissue inhibitor of metalloproteinase (TIMP) were assessed.Results:  The collagen production of fibroblasts decreased when cocultured with keratinocytes; when cocultured with keratinocytes pre‐treated by arecoline, fibroblasts produced more soluble collagen than non‐pretreated coculture group. MMP‐9 was produced only in coculture groups. There was no significant difference in the two coculture groups. The activation ratio of pro‐MMP‐2 in arecoline pre‐treated keratinocytes‐fibroblasts coculture group was significantly higher than that of non‐coculture groups, but no significant difference existed in the two coculture groups. TIMP‐1 produced by arecoline pre‐treated keratinocytes‐fibroblasts coculture group was significantly higher than those by the other three groups.Conclusion:  TIMP‐1 and the interaction of oral keratinocytes and fibroblasts play important role in pathogenesis of OSF.
Differential gene expression profiles of whole lesions from patients with oral lichen planus
Authors: Tao Xiao‐An , Li Chun‐Yang , Xia Juan , Yang Xi , Chen Xiao‐Hua , Jian Yu‐Tao , Cheng Bin 
Published: August 2010

Background:  The molecular biological properties of oral lichen planus (OLP) are largely unknown. The aim of this study was to identify the genes responsible for its pathogenesis at the genome scale using DNA microarray technology.Methods:  The RNA samples extracted from the specimens of nine OLP patients and nine controls were analyzed with Affymetrix GeneChip. Real‐time reverse transcriptase polymerase chain reaction (RT‐PCR) analysis was applied to confirm GeneChip results.Results:  A total of 985 differentially expressed genes (629 up‐regulated and 356 down‐regulated) were identified. These genes were involved in many function classifications and biochemical pathways. The results of quantitative RT‐PCR analysis of FOXP3, VEGFA, ANGPT1, MMP1, and SCGB2A2 were consistent with their changes demonstrated by GeneChip.Conclusions:  This study showed the gene expression profiles of OLP, which were quite distinct from that of healthy controls. These results presented a global view of physiopathologic processes in lesions, which will give important clues to understand pathogenesis and identify new therapeutic targets of OLP.
Expression of mitotic checkpoint proteins BUB1B and MAD2L1 in salivary duct carcinomas
Authors: Ko Young Hyeh , Roh Ji Hyeon , Son Young‐Ik , Chung Man Ki , Jang Jeon Yeob , Byun Hayoung , Baek Chung‐Hwan , Jeong Han‐Sin 
Published: August 2010

J Oral Pathol Med (2010) 39: 349–355Objective:  Defects in the mitotic checkpoint lead to aneuploidy and might facilitate tumorigenesis. However, the ploidy status in salivary duct carcinoma (SDC) has been reported to play limited role in prediction of prognosis. Thus, we need more reliable markers to reflect the rapid tumor progression in SDCs. We aimed here to investigate the expression of mitotic checkpoint proteins benzimidazole 1 homolog beta (BUB1B) and mitosis arrest‐deficient 2 like 1 (MAD2L1) in SDCs and to determine their possible role as surrogate prognostic markers.Methods:  We analyzed the clinical courses, pathologic findings and immunohistochemical profiles of mitotic checkpoint proteins (BUB1B and MAD2L1) in 27 pathologically confirmed SDCs. The expression status of BUB1B and MAD2L1 was compared with clinicopathologic factors and other molecular markers, such as TGF‐beta, c‐erb‐B2, androgen receptor, vascular endothelial growth factor, and epidermal growth factor receptor, for prognostic significance.Results:  High BUB1B expression was detected in 25.9% of subjects, and high MAD2L1 expression was in 55.6% of subjects. However, survival analysis revealed that mitotic checkpoint expression did not have prognostic significance in SDCs, nor did the other studied markers. Rather, the clinical variable of N classification at diagnosis (in N+ status, hazard ratio 5.19, 95% CI 1.26–21.32 for disease‐free survival and hazard ratio 7.18, 95% CI 1.09–46.99 for overall survival) was strongly associated with survival and prognosis based on the Cox proportional hazard model.Conclusions:  Mitotic checkpoint proteins appeared to play a limited role in predicting prognosis in SDCs. Further study is required to elucidate the exact role of mitotic checkpoint proteins in SDCs.
Fatty‐acid‐binding protein 5 promotes cell proliferation and invasion in oral squamous cell carcinoma
Authors: Fang Lei‐Ya , Wong Tung‐Yiu , Chiang Wei‐Fan , Chen Yuh‐Ling 
Published: August 2010

J Oral Pathol Med (2010) 39: 342–348Background:  Oral squamous cell carcinoma (OSCC) is one of the most malignant neoplasms worldwide, and the molecular mechanism of oral tumorigenesis is still unclear. Fatty‐acid‐binding protein 5 (FABP5) was found in our previous study to be upregulated in oral squamous cell carcinomas by proteomic analysis. The implications of FABP5 overexpression in oral cancer progression have not yet been elucidated.Materials and methods:  In this study, the recombinant adeno‐associated virus vectors were used to deliver and increase the expression of FABP5 in human OSCC cell lines. U6 promoter‐driven short‐hairpin RNA (shRNA)‐triggered RNA interference was used to block FABP5 gene expression. Transwell Matrigel invasion assay, MTS cell proliferation assay, reverse transcription‐polymerase chain reaction, Western blot, and gelatin zymography analysis were used to investigate the effects of FABP5 on cell invasion, growth, and matrix metalloproteinase (MMP) production.Results:  Overexpression of FABP5 in oral cancer cells increased cell proliferation and invasiveness by increasing the expression of MMP‐9. Silencing FABP5 with shRNA significantly suppressed cell proliferation, MMP‐9 activities, and invasiveness.Conclusion:  Our study provides the first evidence that FABP5 expression modulated MMP‐9 production and the invasive behavior of oral cancer cells and suggests that FABP5 may provide novel targets for therapeutic intervention.
Oral cancer over four decades: epidemiology, trends, histology, and survival by anatomical sites
Authors: Zini Avraham , Czerninski Rakefet , Sgan‐Cohen Harold D. 
Published: August 2010

J Oral Pathol Med (2010) 39: 299–305Background:  Oral cancer is one of the few life‐threatening oral diseases. The subtypes and different sites of oral cancer has different etiology epidemiology and survival rate. Prevalence of the various anatomical oral sites provided potential baseline for improvement of clinical approach.Methods:  Incidence and survival rates were derived from the Israel National Cancer Registry and included all registered data between 1970 and 2006. Oral cancer included the lips, tongue, buccal mucosa, gums, vestibulum, floor of the mouth, and palate.Results:  Most prevalent oral cancer subtype was squamous cell carcinoma (SCC) among men above the age of 55 years. Females had a higher incidence of SCC in lateral border of tongue, gums and buccal mucosa. Lymphoma and sarcoma were the most prevalent under the age of 20. Melanomas and metastatic disease revealed the lowest survival rate, while invasive or infiltrating basal cell carcinoma in the lips had the highest rate. The highest oral survival rate was for the lip, and the lowest was for the tongue and gums.Conclusions:  Early detection of oral cancer is important for all the medical health team. Decrease in lip carcinoma may be a result of occupational or awareness changes and should be studied. Non‐epithelial tumors under the age of 20 should be considered as a differential diagnosis. A basic oral examination should be included in all routine medical examinations, with emphasis on high‐risk patients and high‐risk oral sites.
ALLO‐AGGRESSION IN CHICKENS
Authors: Simonsen Morten 
Published: August 2010

Simonsen, M. Allo‐aggression in chicken. Serological identification of the major genetic locus. Acta path, microbiol. scand. Sect. C, 83: 1–14, 1975.The B‐group is the conventional name for the major histocompatibility system (MHS) of the chicken and was first described as a blood group locus. It actually comprises an unknown number of linked loci, at least one of which (AA) governs allo‐aggression as manifested, and studied here by the GVH reaction. The gene product(s) of the AA locus would appear to be expressed not only in T cells but also for example in the nucleated erythrocytes, which fact would then in principle permit their detection by haemagglutination. In practice such detection requires that a number of other haemagglutinating antibodies, directed against other antigens of the same B haplotype be first removed, because the unabsorbed antiserum against one given haplotype seems to agglutinate the RBC of almost any unrelated chicken in the world. A strategy is described which allows a planned absorption of the irrelevant specificities from sera against entire haplotypes; hence lead to successful typing for the AA locus. Some observations suggest an extreme degree of linkage disequilibrium in White Leghorns with respect to the genes determining the Bl haplotype, which appears to be a common haplotype in that particular race.
EFFECT OF HALOTHANE ANAESTHESIA ON SECONDARY ANTIBODY RESPONSE AND MITOGEN‐INDUCED LYMPHOCYTE TRANSFORMATION IN THE CHICKEN
Authors: Salo M. , Lassila O. , Viijanen M. , Eskola J. , Kangas L. 
Published: August 2010

The effect of a single halothane anaesthesia on the secondary antibody response and the lymphocyte response in vitro to phytohaemagglutinin (PHA) and concanavalin A (Con A) was studied. The antigens used were bovine serum albumin (BSA) and killed Brucella abortus organisms (Brucella). IgG and IgM antibodies against these antigens were quantified by solid‐phase radioimmunoassay. Lymphocyte transformation was studied by a whole blood micromethod. The chickens were anaesthetised for two hours with 2% v/v halothane. The halothane concentration of the blood was measured by gas chromatography at the end of the anaesthesia.Halothane anaesthesia had no effect on the secondary antibody response against BSA and Brucella. In contrast, it caused a significant decrease in the lymphocyte response to PHA and Con A. This suppression was transient, however, and full recovery of the lymphocyte function was observed by the first day after anaesthesia.