bluuebunny/crossref_metadata_2025_split huggingface dataset:
An easily accessible sulfated saccharide mimetic inhibits in vitro human tumor cell adhesion and angiogenesis of vascular endothelial cells
Authors: Marano Grazia , Gronewold Claas , Frank Martin , Merling Anette , Kliem Christian , Sauer Sandra , Wiessler Manfred , Frei Eva , Schwartz-Albiez Reinhard 
Published: May 2012

Oligosaccharides aberrantly expressed on tumor cells influence processes such as cell adhesion and modulation of the cell’s microenvironment resulting in an increased malignancy. Schmidt’s imidate strategy offers an effective method to synthesize libraries of various oligosaccharide mimetics. With the aim to perturb interactions of tumor cells with extracellular matrix proteins and host cells, molecules with 3,4-bis(hydroxymethyl)furan as core structure were synthesized and screened in biological assays for their abilities to interfere in cell adhesion and other steps of the metastatic cascade, such as tumor-induced angiogenesis.The most active compound, (4-{[(β-D-galactopyranosyl)oxy]methyl}furan-3-yl)methyl hydrogen sulfate (GSF), inhibited the activation of matrix-metalloproteinase-2 (MMP-2) as well as migration of the human melanoma cells of the lines WM-115 and WM-266-4 in a two-dimensional migration assay. GSF inhibited completely the adhesion of WM-115 cells to the extracellular matrix (ECM) proteins, fibrinogen and fibronectin.In an in vitro angiogenesis assay with human endothelial cells, GSF very effectively inhibited endothelial tubule formation and sprouting of blood vessels, as well as the adhesion of endothelial cells to ECM proteins. GSF was not cytotoxic at biologically active concentrations; neither were 3,4-bis{[(β-D-galactopyranosyl)oxy]methyl}furan (BGF) nor methyl β-D-galactopyranoside nor 3,4-bis(hydroxymethyl)furan, which were used as controls, eliciting comparable biological activity. In silico modeling experiments, in which binding of GSF to the extracellular domain of the integrin αvβ3 was determined, revealed specific docking of GSF to the same binding site as the natural peptidic ligands of this integrin. The sulfate in the molecule coordinated with one manganese ion in the binding site.These studies show that this chemically easily accessible molecule GSF, synthesized in three steps from 3,4-bis(hydroxymethyl)furan and benzoylated galactose imidate, is nontoxic and antagonizes cell physiological processes in vitro that are important for the dissemination and growth of tumor cells in vivo.
Efficacy and Tolerance of Levodopa to Treat Amblyopia: A Systematic Review and Meta-Analysis
Authors: Yang Xubo , Luo Deyi , Liao Meng , Chen Bingjie , Liu Longqian 
Published: May 2012

Purpose. Amblyopia is the leading cause of visual impairment in children, affecting up to 4% of the general population. Several clinical trials evaluated the efficacy of levodopa versus placebo in treating amblyopia. However, the results appeared contradictory, suggesting both beneficial and ineffective. The aim of this study was to address the efficacy and tolerance of levodopa on amblyopia. Methods. Randomized controlled trials were identified and extracted from PubMed, EMBASE, Web of Science, and the Cochrane library. The quality of included trials was assessed by using Jadad score and heterogeneity was analyzed using chi-square test. Both efficacy and tolerance endpoints were evaluated. Data were extracted and analyzed using standard meta-analysis. Results. A total of 6 trials were identified from the search strategy. The pooled mean difference of endpoint logMAR of levodopa versus placebo was −0.11 (95% confidence interval −0.19~-0.02, p=0.01). Amblyopic patients receiving levodopa did not show significantly higher frequent adverse events than those receiving placebo. Conclusions. The use of levodopa is an effective and safe option for the treatment of amblyopia, and levodopa can be considered as first-line treatment of amblyopia.
BAT-25 polymorphism in Chinese from Jiangsu province and its implication for locus microsatellite instability screening
Authors: Zheng Yan Ying , Xie Ling , Liu Li , Zhang Shu Peng , Wu Xiao Bin , Le Zhu Chang , Lai Ren Sheng 
Published: May 2012

Background Colorectal cancer is one of the most common tumors with high mortality in China. Microsatellite instability (MSI) analysis is important for the diagnosis of hereditary non-polyposis colorectal cancer (HNPCC) and for the prediction of 5-FU chemotherapy efficiency of colorectal tumors, especially in terms of therapeutic response and overall survival rates. Among the MSI markers recommended by the NIH/NCI, BAT-25 has been extensively studied for its major role in MSI. BAT-25 presents different polymorphisms in different ethnic populations and studies of its polymorphisms in the Chinese population are still very limited. Aims To analyze the frequency of constitutive polymorphic variation at the BAT-25 locus in Chinese from Jiangsu Province and its implication for locus MSI screening. Methods The frequency of allelic variation at the BAT-25 locus of cervical cells from 500 healthy women and blood from 16 healthy males was assessed by direct sequencing. Twenty samples were also analyzed by fragment analysis. DNA extracted from blood of 94 patients with gastrointestinal cancer or endometrial cancer was analyzed by fragment analysis. Results After comparison with the sequencing results, the more frequent allele lengths were 126–127 bp, 128–129 bp, 129–130 bp, respectively consistent with the 24 poly(T) (T24), T25 and T26 alleles. At the BAT-25 locus, 516 healthy individuals had respectively 1.36%, 97.28% and 1.36% of the T24, T25 and T26. Whereas for the 94 cancer patients allelic frequencies were 0.53%, 1.06%, 96.8%, 1.6% for T15, T24, T25 and T26 alleles respectively. Sixteen healthy males had only the T25 allele and heterozygous T15 was only found in 1 male patient with colon cancer. Conclusion We established the relation between fragment length and thymine repeats in BAT-25. The results showed that the BAT-25 locus is quasimonomorphic in Chinese from Jiangsu province. Moreover we showed that variant alleles of BAT-25 were found more likely in blood from cancer patients than in healthy individuals, suggesting the need to perform comparative studies between tumor and blood, or normal tissue, as to obtain a correct MSI identification.
Main Challenges for High Performance NAS Battery: Materials and Interfaces
Authors: Wen Zhaoyin , Hu Yingying , Wu Xiangwei , Han Jinduo , Gu Zhonghua 
Published: May 2012

AbstractThe progress in the research work and real applications of sodium‐sulfur (NAS) battery in large scale energy storage is introduced. The key materials and interfaces of the battery, particularly the role of Shanghai Institute of Ceramics, Chinese Academy of Sciences (SICCAS), are systematically reviewed. As the most important and difficult part, the high‐quality beta‐ alumina ceramic electrolyte tubes are prepared by a low‐cost solid state reaction process; their sealing performance and interfacial behavior with molten sodium and sulfur electrodes could be substantially improved by glass ceramic type sealants and surface modification, respectively. Combination of carbon and additives like SiO2 with different wetting behaviors for sulfur and the discharge product sulfides is shown to be significant in improving the electrochemical performances of NAS battery. Conductive ceramic coatings are developed as anti‐corrosion media of the current collector of sulfur electrode; this is identified as an effective route to protect the metal parts.
Highly Conductive Few‐Layer Graphene/Al2O3 Nanocomposites with Tunable Charge Carrier Type
Authors: Fan Yuchi , Jiang Wan , Kawasaki Akira 
Published: May 2012

AbstractAn ex situ strategy for fabrication of graphene oxide (GO)/metal oxide hybrids without assistance of surfactant is introduced. Guided by this strategy, GO/Al2O3 hybrids are fabricated by two kinds of titration methods in which GO and Al2O3 colloids are utilized as titrant for hybrids of low and high GO content respectively. After sintered by spark plasma sintering, few‐layer graphene (FG)/Al2O3 nanocomposites are obtained and GO is well reduced to FG simultaneously. A percolation threshold as low as 0.38 vol.% is achieved and the electrical conductivity surpasses 103 Sm−1 when FG content is only 2.35 vol.% in FG/Al2O3 composite, revealing the homogeneous dispersion and high quality of as‐prepared FG. Furthermore, it is found that the charge carrier type changes from p‐ to n‐type as graphene content becomes higher. It is deduced that this conversion is related to the doping effect induced by Al2O3 matrix and is thickness‐dependent with respect to FG.
Recent Advances in Azaborine Chemistry
Authors: Campbell Patrick G. , Marwitz Adam J. V. , Liu Shih‐Yuan 
Published: May 2012

AbstractThe chemistry of organoboron compounds has been primarily dominated by their use as powerful reagents in synthetic organic chemistry. Recently, the incorporation of boron as part of a functional target structure has emerged as a useful way to generate diversity in organic compounds. A commonly applied strategy is the replacement of a CC unit with its isoelectronic BN unit. In particular, the BN/CC isosterism of the ubiquitous arene motif has undergone a renaissance in the past decade. The parent molecule of the 1,2‐dihydro‐1,2‐azaborine family has now been isolated. New mono‐ and polycyclic B,N heterocycles have been synthesized for potential use in biomedical and materials science applications. This review is a tribute to Dewar’s first synthesis of a monocyclic 1,2‐dihydro‐1,2‐azaborine 50 years ago and discusses recent advances in the synthesis and characterization of heterocycles that contain carbon, boron, and nitrogen.
The surface modification of magnetic poly(methyl acrylate) microspheres with dendron and application in Au(III) adsorption
Authors: Liu Xiang , Guan Yueping , Wang Qiang , Ren Xiufeng , Yang Mingzhu 
Published: May 2012

AbstractAn improved suspension polymerization method for preparation of the magnetic poly(methyl acrylate) microspheres (mPMA‐DVB) was investigated. Through subsequent reaction with methyl acrylate (MA) and ethylenediamine (EDA), the magnetic poly(methyl acrylate) microspheres with dendron surface was obtained, and the magnetic poly(methyl acrylate) microspheres with dendron surface reacted with carbon bisulfide and sodium hydroxide to create sodium dithiocarbamate. Following, the resultant magnetic microspheres with dendron surface modification were used to adsorb Au(III) from aqueous solution. The result showed that the capacity of amino groups on the surface of the mPMA microspheres increased from 1.67 mmol/g for the magnetic polymer microspheres with G0 dendron to 4.35 mmol/g with G3 dendron, and the adsorption capacity rose from 0.1981 g/g with G0 dendron to 0.7853 g/g with G3 dendron. The effects of solution pH, the adsorption temperature, the adsorption time, and the initial concentration of Au(III) on the adsorption of Au(III) were studied, the optimum pH for Au(III) adsorption was found at pH = 1, the adsorption capacity achieved the maximum in 60 min, and the adsorption process was endothermic reaction and conformed to pseudo‐second‐order kinetic models. Furthermore, the adsorption process was in accordance with the Langmuir model. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Solution properties of a novel ampholytic polyphenylene sulfide
Authors: Xiao Hui , Tao Rong , Cui Wei , Zhang Shuai , Li Rui Hai 
Published: May 2012

AbstractA polyampholyte was prepared from polyphenylene sulfide (PPS) by sulfonation, followed by bromination, and then the substitution of bromine by a quaternary ammonium group and tertiary amine group. The aqueous solution properties of the polyelectrolyte and polyampholyte with different ionic groups were investigated by viscometric measurement and rheological behavior measurement. The results show that the viscosities of the polyelectrolyte and polyampholyte were not only dependent on the nature of acid and base substituents along the polymer chain but also on the external factors, such as pH and small‐molecule electrolytes. In pure water, the rheological behavior of the polyelectrolyte showed an obvious critical shear rate in this circumstance; the polyampholyte showed a relatively stable shear rate compared with the polyelectrolyte. However, in salt‐saturated water, the viscosity decreased quickly with increasing shear rate. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Mechanical, thermal, and microwave properties of conducting composites of polypyrrole/polypyrrole‐coated short nylon fibers with acrylonitrile butadiene rubber
Authors: Pramila Devi D. S. , Nair Ajalesh. B. , Jabin T. , Kutty Sunil K. N. 
Published: May 2012

AbstractPyrrole was polymerized in the presence of anhydrous ferric chloride as oxidant and p‐toluene sulfonic acid as dopant. Polypyrrole‐coated short nylon fibers were prepared by polymerizing pyrrole in the presence of short nylon fibers. The resultant polypyrrole (PPy) and PPy‐coated nylon fiber (F‐PPy) were then used to prepare rubber composites based on acrylonitrile butadiene rubber (NBR). The cure pattern, direct current (DC) conductivity, mechanical properties, morphology, thermal degradation parameters, and microwave characteristics of the resulting composites were studied. PPy retarded the cure reaction while F‐PPy accelerated the cure reaction. Compared to PPy, F‐PPy was found to be more effective in enhancing the DC conductivity of NBR. The tensile strength and modulus values increased on adding PPy and F‐PPy to NBR, suggesting a reinforcement effect. Incorporation of PPy and F‐PPy improved the thermal stability of NBR. The absolute value of the dielectric permittivity, alternating current (AC) conductivity, and absorption coefficient of the conducting composites prepared were found to be much greater than the gum vulcanizate. PPy and F‐PPy were found to decrease the dielectric heating coefficient and skin depth significantly. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
A novel honey‐based nanofibrous scaffold for wound dressing application
Authors: Maleki H. , Gharehaghaji A. A. , Dijkstra P. J. 
Published: May 2012

AbstractIn this study, nanofiber meshes were produced from aqueous mixtures of poly(vinyl alcohol) (PVA) and honey via electrospinning. The Electrospinning process was performed at different PVAs to honey ratios (100/0, 90/10, 80/20, 70/30, and 60/40). Dexamethasone sodium phosphate was selected as an anti‐inflammatory drug and incorporated in the electrospinning solutions. Its release behavior was determined. Uniform and smooth nanofibers were formed, independent of the honey content. In case honey content increased up to 40%, some spindle‐like beads on the fibers were observed. The diameter of electrospun fibers decreased as the ratio of honey increased. The release characteristics of the model drug from both the PVA and PVA/honey (80/20) nanofibrous mats were studied and statistical analysis was performed. All electrospun fibers exhibited a large initial burst release at a short time after incubation. The release profile was similar for both PVA and PVA/honey (80/20) drug‐loaded nanofibers. This study shows that an anti‐inflammatory drug can be released during the initial stages and honey can be used as a natural antibiotic to improve the wound dressing efficiency and increase the healing rate. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci, 2013
Preparation and characterization of several types of polyvinyl butyral hollow fiber membranes by thermally induced phase separation
Authors: Kitaura Toshihiko , Fadzlina Wan Nur , Ohmukai Yoshikage , Maruyama Tatsuo , Matsuyama Hideto 
Published: May 2012

AbstractHollow fiber membranes were prepared by thermally induced phase separation from three types of polyvinyl butyral (PVB) and a blend of two of these polymers. Although the difference in the chemical composition of the PVB polymers used was not remarkable, their respective membrane performances were quite different. With a high phase separation temperature the pore size of the prepared membrane was large, because structure growth occurred for a long time. Water permeability tests of the wet membranes showed the results that corresponded to the pore sizes of the membranes. By contrast, the results for the dried membrane appeared to be related to the hydrophilicity of the PVB polymer and independent of pore size in the wet condition. Although the membrane with high wettability had low mechanical strength, the membrane from the polymer blend of two different PVB polymers showed adequate wettability and mechanical strength. This produced a hollow fiber membrane with favorable characteristics for application in water treatment. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Antarctic ozone depletion and trends in tropopause Rossby wave breaking
Authors: Ndarana T. , Waugh D. W. , Polvani L. M. , Correa G. J. P. , Gerber E. P. 
Published: May 2012

AbstractTrends in summer tropopause Rossby wave breaking (RWB) are examined using meteorological reanalyses and model integrations. The reanalyses for the last 30 years show large increases in RWB on the equatorward side of the tropospheric jet and weak decreases on the poleward side. Comparable changes in RWB are found in general circulation model integrations whose stratospheric ozone differs between 1960 and 2000 levels, but not in integrations that differ only in their greenhouse gas concentrations and sea‐surface temperatures. These results indicate that the formation of the ozone hole has led to changes in RWB frequency during southern summer. Copyright © 2012 Royal Meteorological Society
Maternal tea consumption during early pregnancy and the risk of spina bifida
Authors: Yazdy Mahsa M. , Tinker Sarah C. , Mitchell Allen A. , Demmer Laurie A. , Werler Martha M. 
Published: May 2012

AbstractStudies have demonstrated that catechin, an antioxidant found in tea, can reduce the bioavailability of folate. Because periconceptional folic acid intake has been demonstrated to reduce the risk of spina bifida, tea consumption may put pregnant women at risk because of its possible antifolate properties. Using data collected in the Slone Epidemiology Center Birth Defects Study, we examined whether tea consumption during early pregnancy was associated with an increased risk of spina bifida. Mothers of 518 spina bifida cases and 6424 controls were interviewed within 6 months after delivery about pregnancy events and exposures. Data on tea intake were collected during three periods (1976–1988, 1998–2005 and 2009–2010). Logistic regression models were used to calculate odds ratios (ORs) and 95% confidence intervals (CIs), adjusted for study center. Intake of both periconceptional food folate and diet and supplemental folic acid were examined as a potential effect modifier. For 1976 to 1988, ORs were not elevated for daily tea intake. For 1998 and onward, ORs were also close to 1.0, but there was a modest increase for those who drank more than 3 cups/day (OR, 1.92; 95% CI, 0.84–4.38). Among women with total folic acid intake greater than 400 μg, consumption of 3 cups or more of tea per day was associated with an increased risk of spina bifida in 1976 to 1988 (OR, 2.04; 95% CI, 0.69–7.66) and in the later periods (OR, 3.13; 95% CI, 0.87–11.33). Our data do not support an overall association between tea consumption and spina bifida, but there is a suggestion of a possible interaction between higher levels of folic acid intake and tea consumption. Birth Defects Research (Part A) 2012. © 2012 Wiley Periodicals, Inc.
An ultra scale‐down approach to assess the impact of the choice of recombinant P. pastoris strain on dewatering performance in centrifuges
Authors: Lopes Adriana G. , Khan Nadia , Liddell John , Keshavarz‐Moore Eli 
Published: May 2012

AbstractPichia pastoris is becoming a desirable host in the biopharmaceutical industry for therapeutics production. It grows on methanol to high cell densities ≥100 g DCW/L and secretes foreign proteins at high titers. However, the culture conditions to reach high cell densities pose a challenge to the processability by primary recovery operations, in particular centrifugation, used for cell removal. This work aims to assess the impact of recombinant P. pastoris strain selection on centrifugal dewatering. Normally, the choice of P. pastoris recombinant strain is based on best target protein expression levels; however, it is unknown whether the choice of strain will have an impact on performance of centrifugation operation. To achieve this aim, a previously developed laboratory ultra‐scale down (USD) methodology that successfully predicted centrifugal dewatering of pilot‐scale disk‐type machines, was used in this work. Two recombinant P. pastoris strains, namely a X‐33 and a glycoengineered Pichia strain, were used to perform fermentations secreting different products. The resulting harvested fermentation culture properties were analyzed and the dewatering performances of a pilot‐ and a large‐scale disk‐type centrifuge were evaluated using the USD methodology. The choice of P. pastoris strain was found to have a considerable impact on dewatering performance, with P. pastoris X‐33 strain reaching better dewatering levels than the glycoengineered strain. The USD method proved to be a useful tool to determine optimal conditions under which the large scale centrifuge needed to be operated, reducing the need for repeated pilot‐scale runs during early stages of process development for therapeutic products. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 28: 1029–1036, 2012
Prospective, multicenter European study of the GORE flow reversal system for providing neuroprotection during carotid artery stenting
Authors: Nikas Dimitrios , Reith Wolfgang , Schmidt Andrej , Duda Stephan , Mathias Klaus , Cremonesi Alberto , Dill Harold , Formgren Johan , Pieniazek Piotr , Musialek Piotr , Hornung Marius , Sievert Horst , Reimers Bernhard 
Published: May 2012

AbstractBackgroundEmbolic protection devices (EPDs) are used to provide protection against brain embolization during carotid artery stenting (CAS) to treat carotid artery stenosis, but the optimal type of EPD has not been determined. Distally positioned filters are commonly used but do not provide protection during crossing of the lesion. This prospective, multicenter study investigated a proximally placed device (GORE Flow Reversal System) that reverses blood flow in the internal carotid artery during CAS, thereby directing emboli away from the brain.MethodsOutcomes in 122 patients (28% symptomatic), who underwent CAS using the flow reversal system were assessed (intention‐to‐treat analysis). The primary endpoint was a major adverse event (MAE; defined as death or stroke) within 30 days of CAS. The secondary endpoint was a myocardial infarction (MI) or nonstroke‐related neurologic event within 30 days.ResultsThe flow reversal system could not be used in one patient because of severe vessel tortuosity and in two patients (1.6%) because of intolerance. The 30‐day MAE rate and the secondary endpoint rate were each 1.6%. No patient in the series died or had an MI within 30 days. No patient who was symptomatic before CAS had an MAE. One symptomatic and one asymptomatic patient had a transient ischemic attack.ConclusionsUse of the GORE Flow Reversal System during CAS had a high rate of technical success and low 30‐day rates of adverse neurologic and cardiac events. © 2012 Wiley Periodicals, Inc.
Organocatalytic 1,4‐Addition Reaction of α,β‐γ,δ‐Diunsaturated Aldehydes versus 1,6‐Addition Reaction
Authors: Hayashi Yujiro , Okamura Daichi , Umemiya Shigenobu , Uchimaru Tadafumi 
Published: May 2012

AbstractAmong 1,4‐addition and 1,6‐addition reactions, 1,4‐addition reaction is the main reaction mode in the Michael reaction of α,β‐γ,δ‐diunsaturated aldehyde with several nucleophiles catalyzed by diphenylprolinol silyl ether via iminium ion intermediate. The 1,4‐addition products, which possess excellent enantioselectivity and alkene moiety, are useful chiral building blocks. The ab initio calculation indicates that 1,4‐addition reaction is preferable in terms of π‐orbital coefficient and Mulliken/CHelpG atomic charge.