bluuebunny/crossref_metadata_2025_split huggingface dataset:
Blood flow–restricted walking does not result in an accumulation of metabolites
Authors: Loenneke Jeremy P. , Thrower Austin D. , Balapur Abhishek , Barnes Jeremy T. , Pujol Thomas J. 
Published: October 2011

SummaryThe American College of Sports Medicine recommends lifting a weight of at least 70% of one’s concentric one repetition maximum to achieve muscular hypertrophy as it is believed that anything below this intensity does not produce significant muscle growth. Recent studies have found muscle hypertrophy to occur with low‐intensity ‘aerobic–like’ exercise with the application of blood flow restriction (BFR) to the limbs. Previous research with low load resistance training has shown that elastic knee wraps provide a practical means to induce elevations in whole blood lactate (WBL), which has been hypothesized to result in many of the adaptations observed with this type of exercise. However, this has yet to be investigated with low‐intensity walking. Thus, the purpose of this paper was to determine the degree to which WBL increases with practical BFR walking. Exercise consisted of five 2‐min bouts of walking at 75 m per min on a motor‐driven treadmill with a 1‐min rest period following each exercise bout. Participants completed the walking with (BFR) and without [control (CON)] restriction to the upper thigh in a randomized order. Practical BFR with elastic knee wraps did statistically increase WBL compared with CON; however, this was not considered a real change because the minimal difference between conditions was not exceeded. In conclusion, metabolic stress is not increased following practical BFR walking exercise. This study may provide an explanation for the lower hormone response observed with BFR walking and provide further evidence that mechanisms other than metabolic accumulation exist with BFR.
The use of transcutaneous oximetry to predict complications of chronic wound healing: A systematic review and meta‐analysis
Authors: Arsenault Kyle A. , McDonald Joey , Devereaux P. J. , Thorlund Kristian , Tittley Jacques G. , Whitlock Richard P. 
Published: October 2011

AbstractTranscutaneous oximetry (PtcO2) has been proposed as a method to predict nonhealing of chronic wounds and the occurrence of healing complications. However, the validity of this method as an independent predictor and an optimal threshold value for this tool are poorly defined. We undertook a systematic review and meta‐analysis to address these questions, searching five major medical databases, relevant review articles, and reference lists. We included all studies that used multivariable analysis to evaluate PtcO2 for its ability to predict chronic wound healing complications. Article selection and data abstraction were conducted independently and in duplicate. Four studies, enrolling 901 patients with 910 lower extremity chronic wounds, met our inclusion criteria. These studies showed that a peri‐wound PtcO2 level below a cutoff of 20 mmHg or 30 mmHg was an independent predictor of chronic wound healing complications (odds ratio 3.21, 95% confidence interval 1.07–9.69, I2 = 77%). A threshold value of 20 mmHg was used most frequently in the included studies and showed the strongest association. This review was limited by the small number of studies, a possible reporting bias, and heterogeneity. These results suggest that PtcO2 measurements have independent prognostic value in the assessment of chronic wounds, but further research is required to confirm these findings and define an optimal threshold value.
Constraining cooling histories: rutile and titanite chronology and diffusion modelling in NW Bhutan
Authors: WARREN C. J. , GRUJIC D. , COTTLE J. M. , ROGERS N. W. 
Published: October 2011

AbstractU–Pb analyses of rutile and titanite commonly yield ages that constrain the timing of cooling rather than the timing of their crystallization. Rutile which grew at or close to peak temperature conditions in a mafic granulite, intermediate granulite and mafic amphibolite within juxtaposed litho/tectonostratigraphic units in the Greater Himalayan Sequence (GHS) of NW Bhutan yield LA–MC–ICP–MS U–Pb lower intercept cooling ages of 10.1 ± 0.4, 10.8 ± 0.1 and 10.0 ± 0.3 Ma, respectively. Numerical finite‐difference diffusion models constrained by previously published temperature–time and Pb diffusion data suggest that these ages are best explained by rapid cooling from peak temperature conditions of ∼800 °C at 14 Ma in the granulite‐bearing unit and ∼650 °C at 12 Ma in the amphibolite‐bearing unit. The good fit between the model and analysed ages confirms the relatively high retention of Pb in rutile suggested by the experimental data. Titanite that grew during an exhumation‐related amphibolite facies overprint on an eclogite facies mineral assemblage from the neighbouring Jomolhari Massif yields a U–Pb lower intercept cooling age of 14.6 ± 1.2 Ma. Diffusion modelling suggests that this age is too old to be consistent with the temperature–time paths inferred for the rutile‐bearing samples. Instead, the titanite age suggests cooling from ∼650 °C at an earlier time of 17–15 Ma, implying that the high‐grade rocks in the Jomolhari Massif experienced a different cooling history from the rest of the GHS in NW Bhutan. Together these data show that high‐grade rocks from three apparently different structural levels of the GHS in NW Bhutan experienced rapid cooling at >40 °C Ma−1 at varying times. The highest grade granulite facies rocks were exhumed from deeper structural levels that are not exposed, not preserved, or not yet recognized west of eastern Nepal. A progressive along‐strike change in tectonic regime, metamorphic history and/or exhumation mechanism across the orogen is implied by these thermochronologic data.
Electrophysiological Mapping of Embryonic Mouse Hearts: Mechanisms for Developmental Pacemaker Switch and Internodal Conduction Pathway
Authors: YI TONGYIN , WONG JOHNSON , FELLER ERIC , SINK SAMANTHA , TAGHLI‐LAMALLEM OUARDA , WEN JIANYAN , KIM CHANGSUNG , FINK MARTIN , GILES WAYNE , SOUSSOU WALID , CHEN HUEI‐SHENG V. 
Published: October 2011

Electrical Mapping of Embryonic Mouse Hearts. Introduction: Understanding sinoatrial node (SAN) development could help in developing therapies for SAN dysfunction. However, electrophysiological investigation of SAN development remains difficult because mutant mice with SAN dysfunctions are frequently embryonically lethal. Most research on SAN development is therefore limited to immunocytochemical observations without comparable functional studies.
Methods and Results: We applied a multielectrode array (MEA) recording system to study SAN development in mouse hearts acutely isolated at embryonic ages (E) 8.5–12.5 days. Physiological heart rates were routinely restored, enabling accurate functional assessment of SAN development. We found that dominant pacemaking activity originated from the left inflow tract (LIFT) region at E8.5, but switched to the right SAN by E12.5. Combining MEA recordings and pharmacological agents, we show that intracellular calcium (Ca2+)‐mediated automaticity develops early and is the major mechanism of pulse generation in the LIFT of E8.5 hearts. Later in development at E12.5, sarcolemmal ion channels develop in the SAN at a time when pacemaker channels are down‐regulated in the LIFT, leading to a switch in the dominant pacemaker location. Additionally, low micromolar concentrations of tetrodotoxin (TTX), a sodium channel blocker, minimally affect pacemaker rhythm at E8.5–E12.5, but suppress atrial activation and reveal a TTX‐resistant SAN‐atrioventricular node (internodal) pathway that mediates internodal conduction in E12.5 hearts.
Conclusions

:
Using a physiological mapping method, we demonstrate that differential mechanistic development of automaticity between the left and right inflow tract regions confers the pacemaker location switch. Moreover, a TTX‐resistant pathway mediates preferential internodal conduction in E12.5 mouse hearts. (J Cardiovasc Electrophysiol, Vol. 23 p. 309‐318, March 2012.)
Natural History of Side Branches Jailed by Drug‐Eluting Stents
Authors: YAMAWAKI MASAHIRO , MURAMATSU TOSHIYA , ARAKI MOTOHARU , HIRANO KEISUKE , NAKANO MASATSUGU , ISHIMORI HIROSHI , ITO YOSHIAKI , MURASATO YOSHINOBU , UENO TAKAFUMI , TSUKAHARA REIKO 
Published: October 2011

Background:
Stent deployment across side branch (SB) ostium is common in daily practice. The present study investigated the natural history of SBs jailed by drug‐eluting stents (DES).
Methods:
The thrombolysis in myocardial infarction (TIMI) flow grades of 271 consecutive SBs jailed by DES in 196 patients was assessed immediately after the procedure and at 9 months of follow‐up. Patients receiving any SB intervention were excluded.
Results:
Of 271 jailed SBs, occlusion occurred in 6.27% and deterioration of flow occurred in 6.27% immediately after stenting. In patients with these SB changes, periprocedural myocardial infarction was more likely than in those without (10.0% vs. 1.8%, P = 0.017), while there was no increase of cardiac death or life‐threatening complications such as stent thrombosis and Q‐wave myocardial infarction (Q MI) during follow‐up. At 9 months, angiography showed that one‐third of the initially obstructed SBs were still occluded. In contrast, flow was maintained in almost all (98.6%) SBs with early TIMI flow grade 3 and there was no delayed occlusion of these branches. Multiple regression analysis showed that lesion complexity (Medina bifurcation class, calcification, and preprocedural TIMI grade 2 flow in the SB) and technical factors (jailing by overlapping stents) were related to SB occlusion or flow deterioration.
Conclusions:
Jailed SBs showing good flow after stenting had a favorable angiographic and clinical outcome after 9 months of follow‐up. However, preprocedural lesion complexity and technical factors should be considered to avoid SB occlusion/flow deterioration associated with periprocedural myocardial infarction. (J Interven Cardiol 2012;25:37–46)
Percutaneous Drainage of Postoperative Pericardial Effusion in Cardiac Surgery
Authors: JAUSSAUD NICOLAS , BOIGNARD AUDE , DURAND MICHEL , BACH VINCENT , PORCU PAOLO , HACINI RACHID , BLIN DOMINIQUE , CHAVANON OLIVIER 
Published: October 2011

Background and aim of the study:
Postoperative pericardial effusion is frequent and can be complicated by cardiac tamponade. Although the different drainage techniques are well described in the setting of medical effusion, there is not a standard postoperative effusion treatment. The aim of this work was to assess the feasibility and effectiveness of the percutaneous pericardial drainage.
Methods:
This a retrospective study involving 197 patients from 1990 to 2008. Drainage was performed by subxiphoid puncture (91.9%) or left parasternal puncture (8.1%) between 3 and 690 days following a cardiac procedure via median sternotomy. Effusion thickness was at least 10 mm in the subcostal echocardiography view.
Results:
No deaths directly related to the procedure were observed. Complete and enduring drainage was achieved in 158 patients (80.2%). The procedure failed for 22 patients (11.2%) because no fluid was drained in 14 cases (7.1%) and a right ventricular puncture in 8 cases (4.1%). Recurrence of the effusion, which occurred for 17 patients (8.6%), was more frequent if an effusion of more than 5 mm persisted after the first drainage (P = 0.024) and if the drainage was performed outside the operating room because of emergency (P = 0.046). Risk factors for mortality were recurrence of the effusion (P = 0.04) and drainage performed outside the operating room (P = 0.007).
Conclusions: Percutaneous pericardial drainage is effective to treat postoperative pericardial effusion. When the effusion is thicker than 10 mm and accessible, it can be the initial strategy and surgical drainage can serve as an alternate strategy in case of failure and complications of this procedure. (J Interven Cardiol 2012;25:95–101)
Tunneled‐cuffed catheter implanted into the accessory hemiazygos vein because of occlusion of the left innominate vein
Authors: Letachowicz Krzysztof , Kołodziej Marian , Międzybrodzki Krzysztof , Letachowicz Waldemar , Weyde Wacław , Sąsiadek Marek , Klinger Marian 
Published: October 2011

AbstractHemodialyzed patients are at risk of multiple catheterizations. Nephrologists performing such procedures need to be familiar with congenital and acquired vascular abnormalities. We describe a successful insertion and use of a cuffed‐tunneled catheter in a patient with unusual anatomy of the central venous system. Computed tomography angiography revealed thrombosis of the right subclavian vein and bilateral occlusion of innominate veins. The left internal jugular and subclavian veins joined to form a large vessel that drained through the accessory hemiazygos and azygos veins into the superior vena cava. The catheter was implanted through the left internal jugular vein into the accessory hemiazygos vein. The presented case demonstrates that the catheter can be implanted into distended collateral, especially when no other location is possible.
Acanthamoeba Belonging to T3, T4, and T11: Genotypes Isolated from Air‐Conditioning Units in Santiago, Chile
Authors: Astorga Berbeli , Lorenzo‐Morales Jacob , Martín‐Navarro Carmen M , Alarcón Verónica , Moreno Johanna , González Ana C. , Navarrete Elizabeth , Piñero José E. , Valladares Basilio 
Published: October 2011

ABSTRACTFree‐living amoebae (FLA) of the genus Acanthamoeba are widely distributed in the environment, in the air, soil, and water, and have also been isolated from air‐conditioning units. The objective of this work was to investigate the presence of this genus of FLA in the air‐conditioning equipment at the Institute of Public Health of Chile in Santiago, Chile. Water and air samples were collected from air‐conditioning systems and were checked for the presence of Acanthamoeba spp. Positive samples were further classified at the genotype level after sequencing the highly variable diagnostic fragment 3 (DF3) region of the 18S rRNA gene. This is the first report of the T3, T4, and T11 genotypes of Acanthamoeba in air‐conditioning units from Chile. Overall, the widespread distribution of potentially pathogenic Acanthamoeba strains in the studied source demands more awareness within the public and health professionals in Chile as this pathogen is emerging as a risk for human health worldwide.
Potential impact of surgical periodontal therapy on oral health‐related quality of life in patients with periodontitis: a pilot study
Authors: Saito Atsushi , Ota Kei , Hosaka Yasuo , Akamatsu Mayako , Hayakawa Hiroki , Fukaya Chie , Ida Atsushi , Fujinami Koushu , Sugito Hiroki , Nakagawa Taneaki 
Published: October 2011

AbstractAimTo gain insight into the impact of periodontal surgery on oral health‐related quality of life (QoL) of patients with periodontitis.Material and MethodsStudy participants were recruited from moderate to severe periodontitis patients. After initial periodontal therapy, participants received periodontal surgery. Oral Health‐related Quality of Life Model for Dental Hygiene (OHRQL) was used to assess participant's QoL at each time point of periodontal evaluation (baseline, at least 3 weeks after initial therapy and 3–4 months after surgery).ResultsA total of 21 patients completed OHRQL assessment after surgery. Compared with baseline, a progressive improvement in periodontal parameters was observed during the periodontal therapy. The total mean OHRQL score at baseline (25.5 ± 11.4) was significantly reduced (improved) after initial therapy and after surgery (16.7 ± 9.5 and 15.0 ± 9.7, respectively; p < 0.01). However, no significant difference was found between the OHRQL score after initial therapy and that after surgery.ConclusionsNo significant differences in patients’ oral health‐related QoL were observed between post‐initial therapy and post‐surgery intervals, although a tendency of surgery to determine an improvement in QoL was observed when compared with post‐initial treatment.
Autoantibody‐mediated complement activation on platelets is a common finding in patients with immune thrombocytopenic purpura (ITP)
Authors: Najaoui Abderrahim , Bakchoul Tamam , Stoy Johanna , Bein Gregor , Rummel Mathias J. , Santoso Sentot , Sachs Ulrich J. 
Published: October 2011

AbstractBackground: It is commonly accepted that antibody‐mediated removal of platelets represents a major mechanism of platelet destruction in immune thrombocytopenic purpura (ITP). Although complement activation may participate in platelet clearance, frequency and specificity of complement activation have not yet been studied systematically in ITP. Patients and methods: We examined blood samples from 240 patients with ITP. Samples were assessed for the presence of free and bound platelet autoantibodies by a standard glycoprotein‐specific assay (monoclonal antibody‐specific immobilization of platelet antigens). The ability of all sera to fix complement to a panel of human platelets was investigated in a complement fixation (CF) assay. Fixation of C1q to isolated GP IIb/IIIa was assessed by flow cytometry. Results: Glycoprotein‐specific autoantibodies were detected as platelet‐bound antibodies in 129 (54%) and as additional free antibodies in 26 (11%) and were undetectable in 111 (46%) patients. Assessing these subgroups for CF, 103 (65%), 21 (81%), and 33 (30%) sera gave positive results. If GP IIb/IIIa was absent from the test platelets, 81 (67%) lost their ability to fix complement; if GP Ib/IX was absent, 37 (30%) lost their ability to fix complement. C1q fixation to immunobeads coated with GP IIb/IIIa was observed in 50% of sera containing anti‐GP IIb/IIIa antibodies. Conclusions: In a significant number of patients with chronic ITP, platelet autoantibodies are capable of activating the classical complement pathway. CF is even present in ITP sera without detectable autoantibodies, indicating that current techniques for autoantibody detection may be insufficient. The major targets for complement‐fixing autoantibodies in ITP are GP IIb/IIIa and GP Ib/IX.
Reliability, sensitivity and responsiveness of the Infant Behavioral Assessment in very preterm infants
Authors: Koldewijn K , van Hus J , van Wassenaer A , Jeukens‐Visser M , Kok J , Nollet F , Wolf M‐J 
Published: October 2011

AbstractAim:  The aim of this study is to investigate the reliability, sensitivity and responsiveness of the Infant Behavioral Assessment (IBA) to evaluate neurobehavioural organization in very preterm infants.Methods:  Videotaped assessments of very preterm infants participating in a recent trial served to evaluate a standardized IBA observation. Inter‐rater reliability was based on 40 videos scored by two independent observers, using percentage agreement and weighted Kappa’s. Sensitivity was evaluated by comparing the IBA results of 169 infants at 35–38 weeks postmenstrual age, dichotomized according to two developmental risk factors. For responsiveness, the effect size (ES) was calculated between 0 and 6 months corrected age in all intervention and control infants and in subgroups of high‐risk intervention and control infants with oxygen dependency ≥28 days.Results:  Inter‐rater agreement was 93% in the total assessment; Kappa agreement was moderate to good in the behavioural categories. Significant differences were found between groups with or without risk factors. Larger differences between ESs in the randomized groups with oxygen dependency ≥28 days than in the total randomized groups reflect the responsiveness of the IBA.Conclusion:  In this study, we found satisfactory to good clinimetric characteristics of the IBA in very preterm born infants.
A systematic review of interventions for reducing pain and distress in children undergoing voiding cystourethrography
Authors: Rao Jia , Kennedy Sean E , Cohen Simon , Rosenberg Andrew R 
Published: October 2011

AbstractVoiding cystourethrography (VCUG) is commonly performed to screen for vesicoureteric reflux or other urological anomalies but has a potential to provoke distress in infants and children. We performed a systematic review of randomized controlled trials of interventions to reduce distress, pain or anxiety during VCUG. Eight trials (591 participants) met the inclusion criteria.Conclusion:  Conscious sedation with midazolam effectively alleviates the distress of VCUG in children older than 1 year of age. Psychological preparation and warmed contrast medium may also be effective. Nitrous oxide 50% may be an alternative to midazolam, but further evidence is needed.
Survival and Chipping of Zirconia‐Based and Metal–Ceramic Implant‐Supported Single Crowns
Authors: Schwarz Stefanie , Schröder Christin , Hassel Alexander , Bömicke Wolfgang , Rammelsberg Peter 
Published: October 2011

ABSTRACTPurpose: The objective of this retrospective study was to compare the incidence of chipping of implant‐supported, all‐ceramic, and metal–ceramic single crowns.Material and Methods: One hundred fifty‐three patients (51.7% male, mean age 55.0 years) received 232 cemented implant‐supported single crowns. One hundred and seventy‐nine crowns had a metal framework (gold alloy) and 53 crowns were all‐ceramic (zirconia framework and glass–ceramic veneer material). Age, gender, kind of cementation, and location of the restorations were assessed as possible factors affecting chipping.Results: During the observation period of up to 5.8 years (mean 2.1 years; standard deviation 1.4), a total of 13 (24.5%) all‐ceramic and 17 (9.5%) metal–ceramic crowns suffered from chipping, a difference that was statistically significant. A total of ten single crowns had to be remade resulting in survival of 86.8% (all‐ceramic) and 98.3% (metal–ceramic). The other possible factors did not have a significant effect on the chipping.Conclusion: Chipping was found to be more frequent for all‐ceramic implant‐supported single crowns. If the reasons for the vulnerability of all‐ceramic crowns remain unknown, implants with all‐ceramic single crowns should generally be recommended with care.
The G protein coupled receptor Gpr153 shares common evolutionary origin with Gpr162 and is highly expressed in central regions including the thalamus, cerebellum and the arcuate nucleus
Authors: Sreedharan Smitha , Almén Markus S. , Carlini Valeria P. , Haitina Tatjana , Stephansson Olga , Sommer Wolfgang H. , Heilig Markus , de Barioglio Susana R. , Fredriksson Robert , Schiöth Helgi B. 
Published: October 2011

The Rhodopsin family of G protein coupled receptors (GPCRs) includes the phylogenetic α‐group consisting of about 100 human members. The α‐group is the only group of GPCRs that has many receptors for biogenic amines which are major drug targets. Several members of this group are orphan receptors and their functions are elusive. In this study we present a detailed phylogenetic and anatomical characterization of the Gpr153 receptor and also attempt to study its functional role. We identified the homologue of Gpr153 in the elephant shark genome and phylogenetic and synteny analyses revealed that Gpr162 and Gpr153 share a common ancestor that split most likely through a duplication event before the divergence of the tetrapods and the teleost lineage. A quantitative real‐time PCR study reveals widespread expression of Gpr153 in the central nervous system and all the peripheral tissues investigated. Detailed in situ hybridization on mouse brain showed specifically high expression in the thalamus, cerebellum and the arcuate nucleus. The antisense oligodeoxynucleotide knockdown of Gpr153 caused a slight reduction in food intake and the elevated plus maze test showed significant reduction in the percentage of time spent in the centre square, which points towards a probable role in decision making. This report provides the first detailed characterization of the evolution, expression and primary functional properties of the Gpr153 gene.Database
Protein sequence data have been deposited to the DDJB database (http://www.ddbj.nig.ac.jp/) with accession numbers BR000916, BR000917 and BR000918 for elephant shark Gpr153_exon 1‐3, Gpr153_exon 4 and Gpr153_exon 5 respectively.