bluuebunny/crossref_metadata_2025_split huggingface dataset:
The influence of olfactory experience on the birthrates of olfactory sensory neurons with specific odorant receptor identities
Authors: Rufenacht Karlin E. , Asson Alexa J. , Hossain Kawsar , Santoro Stephen W. 
Published: June 2024

SummaryOlfactory sensory neurons (OSNs) are one of a few neuron types that are generated continuously throughout life in mammals. The persistence of olfactory sensory neurogenesis beyond early development has long been thought to function simply to replace neurons that are lost or damaged through exposure to environmental insults. The possibility that olfactory sensory neurogenesis may also serve an adaptive function has received relatively little consideration, largely due to the assumption that the generation of new OSNs is stochastic with respect to OSN subtype, as defined by the single odorant receptor gene that each neural precursor stochastically chooses for expression out of hundreds of possibilities. Accordingly, the relative birthrates of different OSN subtypes are predicted to be constant and impervious to olfactory experience. This assumption has been called into question, however, by evidence that the birthrates of specific OSN subtypes can be selectively altered by manipulating olfactory experience through olfactory deprivation, enrichment, and conditioning paradigms. Moreover, studies of recovery of the OSN population following injury provide further evidence that olfactory sensory neurogenesis may not be strictly stochastic with respect to subtype. Here we review this evidence and consider mechanistic and functional implications of the prospect that specific olfactory experiences can regulate olfactory sensory neurogenesis rates in a subtype‐selective manner.
Rapid taxonomic categorization of short, abundant virus sequences for ecological analyses
Authors: Sjodin Anna R. , Willig Michael R. , Rodríguez‐Durán Armando , Anthony Simon J. 
Published: June 2024

AbstractPublic health concerns about recent viral epidemics have motivated researchers to seek novel ways to understand pathogen infection in native, wildlife hosts. With its deep history of tools and perspectives for understanding the abundance and distribution of organisms, ecology can shed new light on viral infection dynamics. However, datasets allowing deep explorations of viral communities from an ecological perspective are lacking. We sampled 1086 bats from two, adjacent Puerto Rican caves and tested them for infection by herpesviruses, resulting in 3131 short, viral sequences. Using percent identity of nucleotides and a machine learning algorithm (affinity propagation), we categorized herpesviruses into 43 operational taxonomic units (OTUs) to be used in place of species in subsequent ecological analyses. Herpesvirus metacommunities demonstrated long‐tailed rank frequency distributions at all analyzed levels of host organization (i.e., individual, population, and community). Although 13 herpesvirus OTUs were detected in more than one host species, OTUs generally exhibited host specificity by infecting a single core host species at a significantly higher prevalence than in all satellite species combined. We describe the natural history of herpesvirus metacommunities in Puerto Rican bats and suggest that viruses follow the general law that communities comprise few common and many rare species. To guide future efforts in the field of viral ecology, hypotheses are presented regarding mechanisms that contribute to these patterns.
Breeding ecology of the Asian openbill in eastern Nepal: Larger trees support higher fledgling success
Authors: Tamang Ganesh , Katuwal Hem Bahadur , Subba Asmit , Singh Nanda Bahadur 
Published: June 2024

AbstractColonial nesting waterbirds in agricultural landscapes have historically received limited research attention, especially in South Asia. For example, the Asian openbill (Anastomus oscitans) is a colonial species that extensively utilizes agricultural landscapes, yet there is a notable lack of substantial studies despite increasing urbanization across these landscapes. We investigated the factors affecting the breeding ecology of Asian openbills in eastern Nepal. We used a grid‐based approach to locate stork colonies and monitored them throughout the breeding season from May to November for three consecutive years (2020–2022). Altogether, we observed a total of 67 active colonies, comprising 4020 active nests, which successfully fledged a total of 7566 chicks. Additionally, most of the colonies were located within areas of human settlements (40%), followed by community forests (33%) and agricultural land (27%). The Asian openbills primarily preferred large trees, such as Bombax ceiba (72%), for nesting. The mean height of nesting trees was approximately 4 m taller, the diameter at breast height was twice as large, and the canopy cover area was three times greater than that of non‐nesting trees. The canopy cover area of trees positively influenced the colony size, while colony size positively influenced the fledgling's success. Our study underscores the significance of large trees in providing sufficient space for accommodating a substantial number of openbill nests and fledglings. These findings have significant implications for conservation efforts to protect large trees along with wetlands and agricultural lands, as crucial measures to ensure the sustainable breeding of this nationally vulnerable species.
How to shorten scientific manuscripts
Authors: Tack Ayco J. M. , Ehrlén Johan , Roslin Tomas 
Published: June 2024

AbstractMany journals have strict word limits, and authors therefore spend considerable time shortening manuscripts. Here, we provide pointers for efficiently doing so while retaining key content. We include general guidance, tips for condensing the different parts of a scientific paper, and advice on what to avoid when shortening manuscripts. We hope that readers will find our guidance helpful.
Bacterioplankton taxa compete for iron along the early spring–summer transition in the Arctic Ocean
Authors: Puente‐Sánchez Fernando , Macías‐Pérez Luis Alberto , Campbell Karley L. , Royo‐Llonch Marta , Balagué Vanessa , Sánchez Pablo , Tamames Javier , Mundy Christopher John , Pedrós‐Alió Carlos 
Published: June 2024

AbstractMicrobial assemblages under the sea ice of the Dease Strait, Canadian Arctic, were sequenced for metagenomes of a small size fraction (0.2–3 μm). The community from early March was typical for this season, with Alpha‐ and Gammaproteobacteria as the dominant taxa, followed by Thaumarchaeota and Bacteroidetes. Toward summer, Bacteroidetes, and particularly the genus Polaribacter, became increasingly dominant, followed by the Gammaproteobacteria. Analysis of genes responsible for microbial acquisition of iron showed an abundance of ABC transporters for divalent cations and ferrous iron. The most abundant transporters, however, were the outer membrane TonB‐dependent transporters of iron‐siderophore complexes. The abundance of iron acquisition genes suggested this element was essential for the microbial assemblage. Interestingly, Gammaproteobacteria were responsible for most of the siderophore synthesis genes. On the contrary, Bacteroidetes did not synthesize siderophores but accounted for most of the transporters, suggesting a role as cheaters in the competition for siderophores as public goods. This cheating ability of the Bacteroidetes may have contributed to their dominance in the summer.
Host‐plant sex and phenology of Buddleja cordata Kunth interact to influence arthropod communities
Authors: González‐Ramírez I. , López‐Gómez V. , Cano‐Santana Z. , Romero Pérez A. , Hernández Cumplido J. 
Published: June 2024

AbstractIntraspecific variation in plants is expected to have profound impacts on the arthropod communities associated with them. Because sexual dimorphism in plants is expected to provide consistent variation among individuals of the same species, researchers have often studied the effect it has on associated arthropods. Nevertheless, most studies have focused on the effect of sexual dimorphism in a single or a few herbivores, thus overlooking the potential effects on the whole arthropod community. Our main objective was to evaluate effects of Buddleja cordata's plant‐sex on its associated arthropod community. We surveyed 13 pairs of male and female plants every 2 months during a year (June 2010 to April 2011). Every sampling date, we measured plant traits (water content and leaf thickness), herbivory, and the arthropod community. We did not find differences in herbivory between plant sex or through time. However, we found differences in water content through time, with leaf water‐content matching the environmental seasonality. For arthropod richness, we found 68 morphospecies associated with female and 72 with male plants, from which 53 were shared by both sexes. We did not observe differences in morphospecies richness; however, we found sex‐associated differences in the diversity of all species and differences on the diversity of the most abundant species with an interesting temporal component. During peak flowering season, male plants showed higher values on both parameters, but during the peak fructification season female plants showed the higher values on both diversity parameters. Our research exemplifies the interaction between plant‐phenology and plant‐sex as drivers of arthropod communities' diversity, even when plant sexual‐dimorphism is inconspicuous, and highlighting the importance of accounting for seasonal variation. We stress the need of conducting more studies that test this time‐dependent framework in other dioecious systems, as it has the potential to reconcile previous contrasting observations reported in the literature.
Genome sequencing, comparative analysis, and gene expression responses of cytochrome P450 genes in Oryzias curvinotus provide insights into environmental adaptation
Authors: Li Ming , Deng Aiping , He Chuanmeng , Yao Zebin , Zhuo Zixuan , Wang Xiu yue , Wang Zhongduo 
Published: June 2024

AbstractThe mangrove fish (Oryzias curvinotus) serves as a model for researching environmental adaptation and sexual development. To further such research, we sequenced and assembled a high‐quality 842 Mb reference genome for O. curvinotus. Comparative genomic analysis revealed 891 expanded gene families, including significantly expanded cytochrome P450 (CYP) detoxification genes known to be involved in xenobiotic defense. We identified 69 O. curvinotus CYPs (OcuCYPs) across 18 families and 10 clans using multiple methods. Extensive RNA‐seq and qPCR analysis demonstrated diverse spatiotemporal expression patterns of OcuCYPs by developmental stage, tissue type, sex, and pollutant exposure (17β‐estradiol (E2) and testosterone (MT)). Many OcuCYPs exhibited sexual dimorphism in gonads, suggesting reproductive roles in steroidogenesis, while their responsiveness to model toxicants indicates their importance in environmental adaptation through enhanced detoxification. Pathway analysis highlighted expanded CYP genes in arachidonic acid metabolism, drug metabolism, and steroid hormone biosynthesis. This chromosome‐level genomic resource provides crucial biological insights to elucidate the functional roles of expanded CYPs in environmental adaptation, sexual development, early life history, and conservation in the anthropogenically impacted mangrove habitats of O. curvinotus. It also enables future ecotoxicology research leveraging O. curvinotus as a pollution sentinel species.
Evaluation of the 2021 ESC recommendations for family screening in hereditary transthyretin cardiac amyloidosis
Authors: Muller Steven A. , Peiró‐Aventin Belén , Biagioni Giulia , Tini Giacomo , Saturi Giulia , Kronberger Christina , Achten Anouk , Dobner Stephan , te Rijdt Wouter P. , Gasperetti Alessio , te Riele Anneline S.J.M. , Varrà Guerino G. , Ponziani Alberto , Hirsch Alexander , Porcari Aldostefano , van der Meer Manon G. , Zampieri Mattia , van der Harst Pim , Kammerlander Andreas , Biagini Elena , van Tintelen J. Peter , Barbato Emanuele , Asselbergs Folkert W. , Menale Silvia , Gräni Christoph , Merlo Marco , Michels Michelle , Knackstedt Christian , Nitsche Christian , Longhi Simone , Musumeci Beatrice , Cappelli Francesco , Garcia‐Pavia Pablo , Oerlemans Marish I.F.J. 
Published: June 2024

AimsThe 2021 European Society of Cardiology (ESC) screening recommendations for individuals carrying a pathogenic transthyretin amyloidosis variant (ATTRv) are based on expert opinion. We aimed to (i) determine the penetrance of ATTRv cardiomyopathy (ATTRv‐CM) at baseline; (ii) examine the value of serial evaluation; and (iii) establish the yield of first‐line diagnostic tests (i.e. electrocardiogram, echocardiogram, and laboratory tests) as per 2021 ESC position statement.Methods and resultsWe included 159 relatives (median age 55.6 [43.2–65.9] years, 52% male) at risk for ATTRv‐CM from 10 centres. The primary endpoint, ATTRv‐CM diagnosis, was defined as the presence of (i) cardiac tracer uptake in bone scintigraphy; or (ii) transthyretin‐positive cardiac biopsy. The secondary endpoint was a composite of heart failure (New York Heart Association class ≥II) and pacemaker‐requiring conduction disorders. At baseline, 40/159 (25%) relatives were diagnosed with ATTRv‐CM. Of those, 20 (50%) met the secondary endpoint. Indication to screen (≤10 years prior to predicted disease onset and absence of extracardiac amyloidosis) had an excellent negative predictive value (97%). Other pre‐screening predictors for ATTRv‐CM were infrequently identified variants and male sex. Importantly, 13% of relatives with ATTRv‐CM did not show any signs of cardiac involvement on first‐line diagnostic tests. The yield of serial evaluation (n = 41 relatives; follow‐up 3.1 [2.2–5.2] years) at 3‐year interval was 9.4%.ConclusionsScreening according to the 2021 ESC position statement performs well in daily clinical practice. Clinicians should adhere to repeating bone scintigraphy after 3 years, as progressing to ATTRv‐CM without signs of ATTRv‐CM on first‐line diagnostic tests or symptoms is common.
Exploring the Pt→Ligand Back‐Donation Through the Rotational Barrier of an Acyclic Diaminocarbene
Authors: Nerli Francesca , Samaritani Simona , Labella Luca , Ciancaleoni Gianluca 
Published: June 2024

AbstractThe importance of the Dewar‐Chatt‐Duncanson model for inorganic chemists cannot be overstated. It provides a simple yet robust framework for understanding metal‐ligand bonding in terms of σ‐donation and π‐back‐donation. Some years ago, a publication (Chem. Eur. J. 2015, 21, 2467) introduced a method to effectively correlate experimental NMR data with back‐donation, using [LAu(ADC)]+/0 complexes (ADC=acyclic diaminocarbene) as case study. Here, we theoretically expand the same method to [L(PH3)(ADC)PtCl]+/0 complexes, to verify whether the method is general and to gain deeper insights into the influence of L on Pt‐ADC bonding, especially in the trans position. Such insights hold potential for guiding rational catalyst design strategies.
Cooperative, Close and Remote Steric Effects on the Structural and Optical Properties of Copper(I) Bis‐Phenanthroline Complexes
Authors: Appleton Jordan L. , Ballerini Lavinia , Choua Sylvie , Gourlaouen Christophe , Ruppert Romain , Mauro Matteo 
Published: June 2024

AbstractThe synthesis as well as the structural, optical and computational characterization of seven new highly hindered homoleptic copper(I) phenanthroline complexes (namely C1–C7) is reported along with the comparison with the benchmark derivatives. Despite limited steric hindrance in direct proximity of the copper(I) coordination site, X‐ray structures show that the higher hindered derivatives of the series display a more optimal tetrahedral geometry with minimal D2 symmetry distortion. A novel remote control of the geometry, with steric hindrance away from the coordination site, leads to a favorable arrangement as demonstrated by structural and computational data. In addition, electrochemical and steady‐state and time‐resolved photophysical studies are presented which further support the beneficial effects on the ground‐state redox and excited‐state properties when both remote and close steric effects are exploited. Indeed, both an increase of the photoluminescence quantum yield and a prolongation of the excited state lifetime is observed for the highly‐substituted derivative C6 compared to benchmark counterparts on account of the reduced excited‐state flattening distortions imparted by the additional steric constraints.
Magnetically Reusable Fe3O4‐CuO Nanoparticles as Catalyst for the Synthesis of Aryl‐Substituted Fluorescent Pyrazines for Fe Sensing and Ink Applications
Authors: Das Dibya , Mohanta Tanmayee , Kumar Jena Ashis 
Published: June 2024

AbstractThe synthesis of substituted pyrazines from readily available methyl aryl/heteroaryl ketones and 1,2‐diamines was achieved using highly stable and reusable ferrite‐stabilized copper nanoparticles (Fe3O4‐CuO) as a catalyst. The reaction proceeds through a cascade imine formation–oxidative cyclization method under an oxygen atmosphere. The nanocatalysts were readily recovered and reused up to three consecutive cycles without any significant decrease in particle size, catalytic activity and reaction yield, as evidenced by analytical techniques such as transmission electron microscopy (TEM) and powder X‐ray diffraction (PXRD). The synthesized pyrazines exhibited photophysical behaviour, displayed large Stokes shifts, and were explored as fluorescent probes for the sensitive as well as selective “turn‐off” detection of iron with a limit of detection of 0.63 μM. The sensing method is also useful for the quantification of iron in spiked water samples. Furthermore, the application of luminescent pyrazines as fluorescent ink was successfully demonstrated.
In DFT We Trust: Exhaustive Exploration of 1,3‐Dipolar Cycloadditions Between Nitrones and Levoglucosenone Exposes a Curious Case of Conformational Dynamics
Authors: Cicetti Soledad , Spanevello Rolando A. , Sarotti Ariel M. 
Published: June 2024

AbstractAn experimental and computational study (including DFT calculations and distortion/interaction analysis) was conducted to assess the effect of the nitrone structure in the outcome of dipolar 1,3‐cycloadditions with levoglucosenone, a biomass derived chiral enone. While B3LYP/6‐31G* (the most popular method for modeling these reactions according to our literature search) provides qualitatively good results, large outliers were found for some systems versus experimental data. An exhaustive exploration of other levels allowed us to determine the most appropriate ones to predict simultaneously reactivity and selectivity. The systematically predicted high exo selectivity by the majority of the levels led us to reconsider our initial assignment for the reaction with the nitrone derived from piperidine, which resulted in the discovery of an interesting case of conformational dynamics.
Iron(ΙΙΙ) Catalyzed Aerobic Cα−Cβ Cleavage of Allylic Alcohols
Authors: Bagkavou Georgia G. , Stathakis Christos I. 
Published: June 2024

AbstractThe regiospecific Cα−Cβ cleavage of allylic alcohols under modified aerobic Mukaiyama hydration conditions is presented. The new methodology can be regarded as an equivalent of a two‐step process, a typical hydration of the parent allylic alcohol followed by a 1,2‐diol cleavage. A broad scope of substrates, both cyclic and acyclic, are applicable, thus introducing substantial structural modification to the core, especially of the former type of substrates. Mechanistic investigation of the reaction reveals the importance of the nearby hydroxyl group for the deviation from the typical Mukaiyama hydration pathway.
Enhanced energy density of high entropy alloy (Fe‐Co‐Ni‐Cu‐Mn) and green graphene hybrid supercapacitor
Authors: Mohanty Gobinda Chandra , Chowde Gowda Chinmayee , Gakhad Pooja , Verma Anu , Das Shubhasikha , Chowdhary Shamik , Bhattacharya Jayanta , K Singh Abhishek , Biswas Koushik , Tiwary Chandra Sekhar 
Published: June 2024

AbstractGiven the growing demand for new materials for supercapacitor applications, high entropy alloys (HEAs) are being extensively investigated. They are an efficient alternative to existing energy sources due to their synergistic contribution from individual element. We demonstrate the development of nanostructured HEA (FeCoNiCuMn) as a cathode material with specific capacitance (Cs) of ~388 F g−1 (5 mV s−1). As anode material, green graphene (rice straw biochar) synthesized using pyrolysis shows a maximum Cs of ~560 F g−1 at similar scan rate (5 mV s−1). A hybrid asymmetric liquid state device was assembled using the FeCoNiCuMn nanostructured HEA and green graphene as electrodes. Utilizing the green source, the device provided a high Cs of 83.22 F g−1 at 2 A g−1. The specific energy of the device was 33.4 Wh kg−1 and specific power of 1.7 kW kg−1. The electrochemical behavior of each element in the high entropy composition was studied through post X‐ray photoelectron spectroscopy and scanning electron microscopic analysis. The chemical behavior of FeCoNiCuMn is further investigated using DFT studies. The enhanced electrochemical properties and synergistic contribution of each element of the HEA is studied via d‐band theory. The current study can be utilized to develop asymmetric hybrid supercapacitors as environmental friendly energy source.
Modelling the fire resistance of cross‐laminated timber rib panels
Authors: Kleinhenz Miriam , Palma Pedro , Just Alar , Frangi Andrea 
Published: June 2024

AbstractA cross‐laminated timber rib panel is a floor system comprising cross‐laminated timber plates rigidly bonded to glued‐laminated timber ‘rib’ beams. A design method was developed to estimate the fire resistance of cross‐laminated timber rib panels, based on a bending load‐carrying model. The bending load‐carrying model calculates the bending load‐carrying capacity of cross‐laminated timber rib panels based on simulated temperature distributions of numerical models. The numerical models were validated against the experimental results of full‐scale fire resistance tests. The proposed design method is based on the overall approach of the current revised draft of EN 1995‐1‐2:2004, that is, prEN 1995‐1‐2:2023, and provides conservative estimates of the bending load‐carrying capacity. The effective width is one of the most important design parameters and its influence was studied in detail. A limit value of 60% of the effective width according to prEN 1995‐1‐1:2023 is proposed as effective width in fire.