bluuebunny/crossref_metadata_2025_split huggingface dataset:
Induction and Characterization of Spreading Depolarizations in Swine Following Superior Sagittal Sinus Occlusion: A Novel Gyrencephalic Model Study
Authors: Porras Renan Sánchez , Cuapio Francisco Leonardo Ramírez , Hernandez Mildred Alejandra Guitérrez , Lagunes Angel Alberto Puig , Palmarola Pablo Albiña , Navarro Juan Manuel López , Guitérrez Marcos Suárez , Peregrino Roberto Díaz , López Diego Alberto Sandoval , Fischer Gregor , Vazifehdan Farzam , Woitzik Johannes , Santos Edgar 
Published: May 2024

Abstract

Cerebral sinus thrombosis, which constitutes a small percentage of all strokes, usually affects young individuals and can lead to venous stroke. Stroke is associated with Spreading Depolarization (SD) waves in brain tissue propagating through the affected areas, causing transient ionic homeostasis and neuronal function disruption. This interaction highlights the complexity of the neurological consequences associated with SD. In this study, we investigated the occurrence of SDs following the occlusion of the superior sagittal sinus (SSS) in a gyrencephalic model, specifically swine. To instigate an occlusion, we surgically clipped the middle third of the SSS. The animals were grouped and monitored using one of three methods: electrocorticography (ECoG) alone, ECoG with intrinsic optical signal (IOS) imaging, or ECoG in conjunction with laser speckle contrast and oxygen imaging (LSCI). Post-mortem, the brains were analyzed using 2,3,5-triphenyl tetrazolium chloride (TTC) staining to check for venous infarction. Our results confirmed the induction of SDs in the gyrencephalic swine brain after SSS occlusion, which was detectable via all monitoring methodologies. SD activity was most frequent in the first-hour post-occlusion, subsequently diminishing. IOS imaging identified four unique hemodynamic responses, while TTC staining indicated no infarction. This research is the first to document SDs in the gyrencephalic swine brain following SSS occlusion, laying the groundwork for future investigations in both animal models and human clinical studies.
Primary Adenocarcinoma of the Spermatic Cord: A Case Report with Review of the Literature
Authors: Sun Qi , Yang Yuan-Zhong , Chen Ya , An Xin , Zhang Yijun 
Published: May 2024

Abstract

Background
Primary malignant neoplasms of the spermatic cord are extremely rare, with most reported cases being sarcomas or metastatic carcinomas. However, primary adenocarcinoma of the spermatic cord has not been previously reported.
Case presentation:
A 34-year-old male with a solid mass in the right spermatic cord, was eventually diagnosed with primary adenocarcinoma. Histological examination revealed a moderately-to-poorly differentiated adenocarcinoma exhibiting glandular, cribriform, or nested growth patterns, characterized by medium to large-sized cells and focal extracellular mucus. Immunohistochemical analysis demonstrated positive staining for CK (AE1/AE3), CK8/18, CK19, MOC31 (EP-CAM), and Ber-EP4, while negative staining was observed for CK7, D2-40, WT-1, MC, PAX-8, NKX3.1, PSA, CEA, TTF-1, and NapsinA. Furthermore, a complete loss of INI-1 expression and consistent BRG1 expression were noted in all tumor cells. Next-generation sequencing revealed SMARCB1 deletion, low tumor mutation burden (TMB-L), and microsatellite stability (MSS).
Conclusion
We reported the first case of primary adenocarcinoma of the spermatic cord with SMARCB1 (INI-1) deficiency. This case contributes to the expanding understanding of rare neoplasms and underscores the importance of further research into therapeutic strategies targeting SMARCB1-deficient tumors.
A fast open data reduction workflow for the electron microprobe flank method to determine Fe3+/ΣFe contents in minerals
Authors: Hezel Dominik C. , Höfer Heidi E. , Fichtner Andreas 
Published: May 2024

Abstract
Knowing the Fe3+/ΣFe ratio in minerals is important to understand the formation and evolution of minerals and their host rocks. Variable Fe3+/ΣFe in, e.g., garnet is accompanied by a change of their characteristic FeLα and FeLβ X-ray emission lines, which can be quantified with electron microprobe measurements using the flank method. Previously, the required data reduction process to determine the Fe3+/ΣFe was complex. Here, we present a new data reduction tool taking garnet data as an example. This new Flank Reduction app is a freely available, graphical user interface (GUI)-driven, web-based application to reduce flank method data quickly and easily. The entire data reduction process is achieved in minutes compared to hours or days, as it was before. Flank Reduction provides comprehensive insights into, e.g., the flank method standards employed, data obtained, and errors through a wide array of controls and visualization tools. Documentation with comprehensive information on the flank method, data reduction, as well as training material such as video tutorials or sample data sets, are available on a dedicated webpage. Flank Reduction emphasizes the high value of Findable, Accessible, Interoperable, Reproducible (FAIR) and open research software and demonstrates how current developments in coding and app implementation can facilitate the development of powerful and expandable research software.
Evaluation of phosphate insecticides and common herbicides: monitoring and risk assessment in water treatment plant, distribution networks, and underground water wells
Authors: Tahmasebi Ali Akbar , Tabatabaei Zeynab , Azhdarpoor Abooalfazl , Salimi Beni Alireza 
Published: May 2024

ABSTRACT
Despite the negative effects that the use of pesticides (such as herbicides and insecticides) have on human health and water resources, a significant portion of the world's agricultural production depends on them. The purpose of this study was to determine selected residual concentrations of pesticides (diazinon, ethion, malathion, alachlor, methyl-parathion, trifluralin, atrazine, chlorpyrifos, and azinphos-methyl) in samples from Shiraz potable water sources. For this purpose, water treatment plant, groundwater wells, treated surface water, and a mixture of groundwater and treated surface water were taken. In addition, statistical and risk analyses (carcinogenic and non-carcinogenic) were used. According to the results, chlorpyrifos with 84.4% had the highest removal efficiency and methyl-parathion with 10% had the lowest removal rate in the Shiraz water treatment plant process. The highest mean concentration was related to azinphos-methyl (1.5 μg/L) and chlorpyrifos (0.59 μg/L) in the groundwater samples. All measured compounds in water source samples were below standard levels, except for chlorpyrifos and azinphos-methyl, which were reported in groundwater above the limit recommended by the Environmental Protection Agency (EPA). The results showed that while the selected pesticides measured had a low non-carcinogenic risk for both adults and children, malathion and trifluralin posed a high carcinogenic risk for adults.
Endotoxin, a novel biomarker for the rapid risk assessment of faecal contamination of coastal and transitional waters
Authors: Good Christian R. , White Alistair , Brandao Joao , Jackson Simon 
Published: May 2024

ABSTRACT

Current methods for testing water for faecal contamination rely on the culture of faecal indicator bacteria (FIB; Escherichia coli and Enterococci) that take 24–48 h, which leads to delays in taking proactive measures and poses a risk to public health. More rapid methods are therefore required. Here, we have tested a rapid, portable assay (Bacterisk) that detects the bacterial biomarker endotoxin in 30 min to quantify the bacterial biomass present, to evaluate 159 coastal water samples and to compare the results with the traditional culture of FIB. There was a significant correlation between the Bacterisk data given in endotoxin risk (ER) units and FIB culture that could accurately distinguish between poor and sufficient or good quality bathing water using the EU bathing directive values. Receiver operating characteristic analysis was used to determine the optimal ER threshold for coastal water samples, and the area under the curve was 0.9176 with a p-value of &lt;0.0001. The optimal threshold was 7,300 ER units with a sensitivity of 95.45% and a specificity of 83.48%. In conclusion, we have shown that the Bacterisk assay provides a rapid and easy-to-use in situ method to assess bathing water quality.
Estimating rainfall-runoff parameters for ungauged catchments: a case study in Turkiye
Authors: Tuna M. Cihat , Aytaç Ayça 
Published: May 2024

ABSTRACT
Determining water yield and flood discharges in catchments is a vital aspect of hydrology. This entails considering precipitation and runoff as key hydrological parameters. Constructing infrastructure like hydroelectric plants and regulators over streams necessitates continuous, accurate flow, and meteorological observations spanning at least 25 years. However, in developing nations, economic factors often impede such observations. This study proposes a method to estimate peak rainfall and flow values for ungauged basins with varying return periods by utilizing from gauged basins and spatial variables. Flood calculations were carried out for the ungauged Rabat River basin. In this study, regional flood frequency analysis was carried out using the flow values of the flow gauging stations neighboring the basin. In addition, maximum flow values were calculated using Moscus and the DSI synthetic method. Two- and three-parameter distributions were used to estimate 50-, 100-, 200- and 500-year flood return values at stations with observation periods ranging from 15 to 64 years. Kolmogorov–Smirnov and Probability Line Correlation coefficient (Chi-square) tests were applied to check the suitability of these distributions and the most appropriate distributions were found. This yielded an estimation for the flow values of the Rabat River, indicating the method's reliability for forecasting runoff-rainfall in the ungauged basin.
Amyloid fibril–bacterial cellulose nanohybrid membrane cartridge for efficient removal of heavy metal from industrial wastewater
Authors: Vaidh Sachin , Surana Aastha , Nagariya Viraj , Rahewar Ravindrasinh , Prajapati Harsh , Patel Dhaval , Pandya Alok , Vishwakarma Gajendra Singh 
Published: May 2024

ABSTRACT

Worldwide, the harmful ion contamination of water has become a serious problem because of unregulated industry, energy production, and mining, which greatly increase the concentration of pollutants in water. The novel membranes through adsorbent self-assembly, such as protein amyloids, were explored for wastewater treatment. Herein, we report amyloid fibril (AF)-embedded bacterial cellulose nanohybrid membrane for efficient removal of heavy metal from industrial effluent. AFs are synthesized by heat treatment using bovine serum albumin and embedded with bacterial cellulose nanomembrane (BCN). The AF-embedded BCN (AF/BCN) was characterized using microscopy and spectroscopic methods. In addition, the well-ordered multi-layered AF/BCN filtration assembly was fabricated in the commercial cartridge and validated for the removal of heavy metals (Pb2+ and Hg2+) from wastewater and treatment of industrial wastewater sample containing heavy metals. Our multi-layered filtration assembly removed Hg2+ and Pb2+ with efficiency of 95 and 78.34%, respectively. A computational study using molecular docking has also been performed for the identification of metal entrapment sites. Moreover, our AF/BCN filtration assembly showed high regeneration capacity up to four cycles. The isotherm model also revealed a strong fit and good adsorption behaviour. This makes potential filtration assembly for low-cost, high-efficiency for the removal of heavy metal from wastewater.
Identifying and Validating an Angiogenesis-related Signature for the
Prognosis of Head and Neck Squamous Cell Carcinoma
Authors: Hou Yueting , Pang Haifeng , Xu Xuemei , Zhao Dong 
Published: May 2024

Aims::
The present study aimed todevelop a prognostic model for HNSCC
treatment on the basis of angiogenesis-related signatures.

Background::
Head and Neck Squamous Cell Carcinoma (HNSCC) is the most frequent
malignancy with poor prognostic outcomes in the head and neck. Angiogenesis plays a
critical role in tumorigenesis and is expected to be an effective therapeutic target.

Objective::
The RNA-seq dataset TCGA-HNSCC and the hallmark gene set were used
for angiogenesis-related RiskScore model construction.

Methods::
The RNA-seq data was downloaded from The Cancer Genome Atlas (TCGA),
and the hallmark gene set was used to measure the angiogenesis score using the
GSVA R package. Then, the optimal cutoff point for prognostic classification was calculated
by the survminer package, and Weighted Gene Co-expression Network Analysis
(WGCNA) was used to identify angiogenesis gene modules . Multi/univariable and Lasso
Cox analyses were performed to develop the RiskScore model, and the classifier efficiency
was evaluated by the Receiver Operating Characteristic curve (ROC). Furthermore,
a nomogram was designed for survival probability prediction, and the immune infiltration
and immunotherapy differences among different risk patients were assessed.

Results::
After calculating the angiogenesis score, we found that this indicator and patients’
prognosis were closely correlated, especially when patients with a high angiogenesis
score had a poor prognosis. Then, WGCNA identified a blue gene module positively
correlated with angiogenesis. Multivariate and Lasso Cox analysis further identified 9
risk model genes for developing a RiskScore, which was used to divide low- and high-
-risk groups of patients. Those with a high risk tended to show poor prognosis, immune
infiltration, and higher immune escape. Finally, a nomogram was developed to optimize
the risk model, and it exhibited excellent short- and long-term survival prediction performance.

Conclusion::
We constructed a reliable RiskScore model for the prognostic prediction of
HNSCC patients, contributing to precise therapeutic intervention of the cancer.
Profiles of Circulating Exosomal Mirna in Patients with SAPHO Patients by High-Throughput Sequencing
Authors: Zhang Yunan , Zhen Jianhua , Sun Yuxiu , Li Yini , Zhou Yali , Li Chen , Tian Lichun 
Published: May 2024

Background:
Synovitis, acne, pustulosis, hyperostosis, and osteitis (SAPHO) syndrome
is a rare disease that is characterized by autoinflammatory lesions on both bones and skin.
The diverse manifestations and limited understanding of its etiology have hindered the diagnosis
and treatment of this condition. SAPHO syndrome is also classified as a primary inflammatory
osteitis. The onset of osteoarticular involvement in this disease is typically gradual, and the identification
of associated biomarkers may be crucial for accurate diagnosis, effective treatment, and
a better understanding of its underlying mechanisms.

Methods:
We enrolled a total of 6 SAPHO patients and 3 healthy volunteers for this study. The
miRNA expression profile in circulating exosomes was analyzed using next-generation sequencing.
A total of 45 miRNAs were found to be differentially expressed in SAPHO patients. Linear
discriminant analysis effect size analysis and Wilcoxon rank-sum test were employed to identify
biomarkers based on these differentially expressed miRNAs. Among them, we selected 4 miRNAs
as biomarkers for SAPHO syndrome, resulting in an area under the receiver operating characteristic
curve of 1.

Results:
The differentially expressed miRNAs indicated enrichment in immune system and endocrine
system-related KEGG pathways, as well as infectious diseases and cancers. Furthermore,
the most significantly enriched molecular functions in GO analysis were protein binding and catalytic
activity.

Conclusion:
The exosomal miRNA profile in SAPHO syndrome exhibited significant changes,
suggesting its potential as a candidate biomarker for diagnostic assistance, although further investigation
is warranted to elucidate their role in the pathology.
The Antimicrobial Activity of Combination of Vitamin D3 and Omega-3 against Pathogenic Microorganisms
Authors: Al-Najjar Mohammad A. A. , Abdulrazzaq Shaymaa B. , Al-Shaer Shatha , Barakat Muna , Omar Amin , Zrieq Rafat , Hasen Eliza , Abu Samak Mahmoud 
Published: May 2024

Background:
Food supplements such as vitamin D3 and omega-3 have a significant
role in activating the immune system and impacting the diversity of gut microbiota; thus, controlling
the growth of invading pathogens indirectly.

Objective:
This study aims to evaluate the direct antimicrobial activity of vitamin D3 and omega-
3 individually, combined together, and combined with low concentrations of gentamicin or
amphotericin B against selected pathogenic microorganisms. In addition, this study hypothesizes
the potential antimicrobial mechanism and recommends suitable studies to be conducted.

Method:
Minimum inhibitory concentration of different serial dilutions of vitamin D3
[0.7μg/mL-83.3μg/mL] or omega-3 [0.7mg/mL-100mg/mL] or combined [vitamin
D3:1.3μg/mL-83.3μg/mL and omega-3:1.56mg/mL-100mg/mL] with/without antibiotic have
been investigated on Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa and
Candida albicans using check board technique.

Results:
The highest concentration of vitamin D3 [83.3 μg/mL] demonstrated a complete eradication
of the tested microorganisms. Conversely, omega-3 had a lower effect on them. The
highest concentration of combining vitamin D3 and omega-3 with/without gentamicin resulted
in a complete eradication of the S. aureus, E. coli and P. aeruginosa with a 6.8 to 7 log reduction.
On the other hand, C. albicans was inhibited when using vitamin D3 [83.3 μg/mL] or when
this concentration is combined with 100mg/mL of omega-3. However, when these two concentrations
were added to amphotericin B the log reduction dropped to 0.45 suggesting antagonistic
effect.

result:
The highest concentration of vitamin D3 (83.3 μg/mL) demonstrated a complete eradication of the tested microorganisms. Conversely, omega-3 had a lower effect on them. The highest concentration of combining vitamin D3 and omega-3 with/without gentamicin resulted in a complete eradication of the S. aureus, E. coli and P. aeruginosa with a 6.8 to 7 log reduction. On the other hand, C. albicans was inhibited when using vitamin D3 (83.3 μg/mL) or when this concentration is combined with 83.3 μg/mL vitamin D3 and 100mg/mL of omega-3. However, when these two concentrations were added to amphotericin B the log reduction dropped to 0.45 suggesting antagonistic effect.

Conclusion:
These findings suggested that, unlike omega 3, vitamin D3 possesses good antimicrobial
effects against pathogenic microorganisms. The combination of the studied food supplement
showed enhanced microbial inhibition at high concentration, while they had antagonistic
effect when combined with amphotericin B and applied on C. albicans combined. Further
studies on the exact antimicrobial mechanism are still required to understand the measured data
here.
Nano-Formulation Approaches to Enhance Transdermal Drug Delivery-An Updated Review of Nanovesicular Carrier “Transethosomes”
Authors: Singh Jitendra Pratap , Saini Geetanjali , Tiwari Gaurav , Singh Bhupendra 
Published: May 2024

Abstract:
Transdermal drug delivery is an attractive and patient-friendly route for administering
therapeutic agents. However, the skin's natural barrier, the stratum corneum, restricts the passage of
many drugs, limiting their effectiveness. To overcome this challenge, researchers have developed
various nanocarriers to enhance drug penetration through the skin. Transethosomes, a novel and
promising drug delivery system, have emerged as an innovative solution for improving transdermal
drug delivery. Transethosomes are a hybrid of two established nanocarriers: ethosomes and transfersomes.
Ethosomes are lipid-based vesicles that can accommodate lipophilic and hydrophilic
drugs, while transfersomes are deformable lipid vesicles designed to enhance skin penetration.
Transethosomes combine the advantages of both systems, making them ideal candidates for efficient
transdermal drug delivery. They are composed of phospholipids, ethanol, and water and exhibit
high flexibility, enabling them to squeeze through the tight junctions of the stratum corneum. This
abstract reviews the key characteristics of transethosomes, including their composition, preparation
methods, mechanisms of action, characterization parameters, and prospects. Moreover, the recent
advancements and applications of transethosomes in delivering various therapeutic agents, such as
analgesics, anti-inflammatories, hormones, and skincare products, are explored. The enhanced skin
penetration capabilities of transethosomes can potentially reduce systemic side effects and improve
patient compliance, making them a valuable tool in the field of transdermal drug delivery. In conclusion,
transethosomes represent a promising platform for overcoming the challenges of transdermal
drug delivery. Their unique properties enable efficient drug permeation through the skin, offering
a more controlled and effective means of administering a wide range of pharmaceutical and
cosmetic products. This abstract highlights the potential of transethosomes as a valuable addition to
the field of transdermal drug delivery and paves the way for further research and development in
this area.
Antimicrobial Therapy in Dentistry: An Evidence-based Review of
Pomegranate Fruit Properties
Authors: Martins Almeida Nara Lígia , Ferreira Peralta Laura Catalí , Soares Lara Vanessa 
Published: May 2024

Background:
Phytotherapy is characterized by the use of medicinal plants in their different
dosage forms. Currently, the use of this alternative medicine has increased. The raw material
of herbal medicines consists of organs from medicinal plants (leaves, stems, fruits, peels,
flowers and roots) with pharmacological, food, technical or cosmetic adjuvant effects. In addition,
the side effects of its use appear to be reduced compared to synthetic and conventional medication.

Objective:
The aim of this mini-review is to present the scientific evidence involving the properties
of the pomegranate fruit for future antimicrobial applications in dentistry.

Methods:
Literature searches included randomized clinical trials and in vitro studies [2000-2023]
filtered mainly by the following terms [Punica granatum L.], [Pomegranate], [Dentistry], [Antimicrobial
activity] and [Candida albicans].

Results:
Studies have shown that Punica granatum Linn. has considerable antimicrobial activity,
in addition to other pharmacological effects such as anti-cancer, antioxidant and antiinflammatory
action. The use of herbal medicines, as potent antimicrobial agents against oral
pathogens, has been widely reported.

Conclusion:
The use of P. granatum has proven its effectiveness, as a potent antimicrobial agent
against oral pathogens, especially against the fungus Candida albicans, future studies need to focus
on the development of a biocompatible formulation directed to the treatment of patients with
oral candidiasis.