bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulation of Integrin Adhesions by Varying the Density of Substrate-Bound Epidermal Growth Factor
Authors: Shahal Tamar , Geiger Benjamin , Dunlop Iain E. , Spatz Joachim P. 
Published: March 2012

Substrates coated with specific bioactive ligands are important for tissue engineering, enabling the local presentation of extracellular stimulants at controlled positions and densities. In this study, we examined the cross-talk between integrin and epidermal growth factor (EGF) receptors following their interaction with surface-immobilized Arg-Gly-Asp (RGD) and EGF ligands, respectively. Surfaces of glass coverslips, modified with biotinylated silane-polyethylene glycol, were functionalized by either biotinylated RGD or EGF (or both) via the biotin–NeutrAvidin interaction. Fluorescent labeling of the adhering A431 epidermoid carcinoma cells for zyxin or actin indicated that EGF had a dual effect on focal adhesions (FA) and stress fibers: at low concentrations (0.1; 1 ng/ml), it stimulated their growth; whereas at higher concentrations, on surfaces with low to intermediate RGD densities, it induced their disassembly, leading to cell detachment. The EGF- dependent dissociation of FAs was, however, attenuated on higher RGD density surfaces. Simultaneous stimulation by both immobilized RGD and EGF suggest a strong synergy between integrin and EGFR signaling, in FA induction and cell spreading. A critical threshold level of EGF was required to induce significant variation in cell adhesion; beyond this critical density, the immobilized molecule had a considerably stronger effect on cell adhesion than did soluble EGF. The mechanisms underlying this synergy between the adhesion ligand and EGF are discussed.
Differential effects of dairy snacks on appetite, but not overall energy intake
Authors: Dougkas Anestis , Minihane Anne M. , Givens D. Ian , Reynolds Christopher K. , Yaqoob Parveen 
Published: March 2012

Dietary regulation of appetite may contribute to the prevention and management of excess body weight. The present study examined the effect of consumption of individual dairy products as snacks on appetite and subsequent ad libitum lunch energy intake. In a randomised cross-over trial, forty overweight men (age 32 (sd 9) years; BMI 27 (sd 2) kg/m2) attended four sessions 1 week apart and received three isoenergetic (841 kJ) and isovolumetric (410 ml) servings of dairy snacks or water (control) 120 min after breakfast. Appetite profile was determined throughout the morning and ad libitum energy intake was assessed 90 min after the intake of snacks. Concentrations of amino acids, glucose, insulin, ghrelin and peptide tyrosine tyrosine were measured at baseline (0 min) and 80 min after the intake of snacks. Although the results showed that yogurt had the greatest suppressive effect on appetite, this could be confounded by the poor sensory ratings of yogurt. Hunger rating was 8, 10 and 24 % (P < 0·001) lower after the intake of yogurt than cheese, milk and water, respectively. Energy intake was 11, 9 and 12 % (P < 0·02) lower after the intake of yogurt, cheese and milk, respectively, compared with water (4312 (se 226) kJ). Although there was no difference in the postprandial responses of hormones, alanine and isoleucine concentrations were higher after the intake of yogurt than cheese and milk (P < 0·05). In conclusion, all dairy snacks reduced appetite and lunch intake compared with water. Yogurt had the greatest effect on suppressing subjective appetite ratings, but did not affect subsequent food intake compared with milk or cheese.
Gastrointestinal nematodes in rotationally grazing ewes in the mountainous region of central Mexico
Authors: Acevedo-Ramírez P.M.C. , Quiroz-Romero H. , Cruz-Mendoza I. , Ulloa-Arvizu R. , Ibarra-Velarde F. 
Published: March 2012

AbstractThe aims of this study were to determine the frequency of egg shedding (percentage of egg-positive faecal samples) and faecal egg counts (FEC) over 13 months in two different breeds of ewes, both pregnant and non-pregnant, in a mountainous region of central Mexico. Additionally, the effect of ivermectin and albendazole treatments on FEC reduction was recorded. The study also aimed to relate temperature and rainfall to FEC. The gastrointestinal nematode (GIN) third-stage larvae genera recovered from both faeces and grassland pastures in a temperate region were also assessed. Faecal samples were collected from ewes at monthly intervals for 13 months to investigate the FEC population of GIN larvae, their concentration and genera in grass samples collected from grazed and rested pastures. Egg-shedding frequency ranged from 0 to 92% and FEC from 0 to 12,000 eggs per g faeces (epg), with counts in Suffolk higher than in Dorset ewes. The identified genera were Haemonchus, Trichostrongylus, Teladorsagia, Cooperia, Oesophagostomum, Bunostomum, Nematodirus and Strongyloides. Haemonchus and Trichostrongylus were the most common genera. The number of L3 was higher in grazing lands than in those at rest. The highest FEC were recorded in the dry season due to peripartum, but the highest L3 counts were recorded in the rainy season. The coexistence of species of different geographical distributions at this site may be because there is a confluence of Nearctic and Neotropical geographic regions; thus, despite the temperate climate, tropical species can be found. Additionally, this study suggests that increasing temperatures could favour the presence of different tropical GIN species together with typical temperate-zone GIN species.
Endoscopic, assisted, modified turbinoplasty with mucosal flap
Authors: Puterman M M , Segal N , Joshua B-Z 
Published: March 2012

AbstractA variety of surgical methods have been developed to reduce the volume of the inferior turbinates, in order to create a more patent nasal airway. We describe a technique used in our department since February 2002 for all patients undergoing inferior turbinectomy. We resect with endoscopic assistance the lateral mucosa and bony inferior turbinate. This technique can reduce a large volume of the turbinate while preserving the mucosal continuity and the submucosa by covering the raw surface with a mucosal flap. We believe our method minimises post-operative side effects and complications such as dryness, infection, bleeding and pain.
Population increase at a calving ground of the Endangered Tibetan antelope Pantholops hodgsonii in Xinjiang, China
Authors: Buzzard Paul J. , Wong How Man , Zhang Huibin 
Published: March 2012

AbstractFemales in most populations of chiru or Tibetan antelope Pantholops hodgsonii migrate up to 350 km each year to summer calving grounds. These migrations characterize the Tibet–Qinghai Plateau. The Ullughusu calving grounds adjacent to the Arjinshan Nature Reserve in Xinjiang, China, are used by multiple chiru populations and this calving ground recovered from major poaching events in 1998 and 1999, with a population increase from 2001 to 2006. We used direct methods (vehicle/walking transects and radial point sampling) and an indirect method (faecal pellet counts) from 30 June to 4 July 2011 to assess the chiru population at this calving ground. We saw substantially more chiru with all methods in 2011 compared to 2006, demonstrating that the population has increased and suggesting that conservation efforts have been effective.
Theory and Praxis: Simone Weil and Marx on the Dignity of Labor
Authors: Sparling Robert 
Published: March 2012

AbstractSimone Weil had an ambivalent attitude toward Marx. While she thought that the young Marx's celebration of labor had “lyrical accents,” she ultimately believed that Marx had neglected his own insights, embracing a blind worship of mechanization and a theory of history and revolution that was insufficiently attentive to the material conditions of workers. Marx, in her view, was insufficiently materialist and excessively wedded to a hierarchical model of science that maintained the domination of management. Weil and Marx's attitudes toward the dignity of labor and the necessary conditions for socialism are analyzed. The most significant cleavage between them is ultimately due to the differing manner in which they conceive of the relationship between thought and action. Through this comparison, the philosophical underpinnings of the two radically different conceptions of labor and its dignity as a human activity are explained.
Variable Phase Dark-Field Contrast—A Variant Illumination Technique for Improved Visualizations of Transparent Specimens
Authors: Piper Timm , Piper Jörg 
Published: March 2012

AbstractVariable phase dark-field contrast has been developed as an illumination technique in light microscopy, which promises significant improvements and a higher variability in imaging of several transparent specimens. In this method, a phase contrast image is optically superimposed on a dark-field image so that a partial image based on the principal zeroth-order maximum (phase contrast) interferes with an image that is based on the secondary maxima (dark field). The background brightness and character of the resulting image can be continuously modulated from a phase-contrast-dominated to a dark-field-dominated character. The condenser aperture diaphragm can be used for modulations of the image's appearance. Specimens can either be illuminated concentrically or obliquely (eccentrically) when parts of the illuminating light beams are covered and blocked. Moreover, a bright-field-like partial image can be added. In this way, the illumination can be optimally adjusted to the specific properties of the specimen. The techniques described can lead to improved visual information especially in biological specimens consisting of phase structures and additional light-absorbing or -reflecting components. Moreover, the specimen's three-dimensionality can be accentuated with improved clarity because the illuminating light beams associated with phase contrast and dark field run to the specimen at different angles of incidence.
Religion and American Public Life
Authors: Olson Laura R. 
Published: March 2012

In his 2000 best seller Bowling Alone: The Collapse and Revival of American Civic Community, Robert Putnam analyzed the links between social capital and civic engagement. Lamenting the decline of “civic America,” he called for a Tocquevillean renewal of voluntary association in the United States. In American Grace, Putnam and coauthor David Campbell—who also helped with the preparation of Bowling Alone—return to the analysis of American civil society, focusing their attention on America's changing religious landscape and its implications for democracy. Their basic argument is that while the United States is religiously diverse and pluralistic to a profound degree, and while in recent years it has witnessed growing religious polarization, it has also succeeded in muting religious tensions and hostilities. As they conclude: “How has America solved the puzzle of religious pluralism—the coexistence of religious diversity and devotion? And how has it done so in the wake of growing religious polarization? By creating a web of interlocking personal relationships among people of many different faiths. This is America's grace” (p. 550).Given the importance of religion in American life and the influence of Putnam's broad agenda on much current social science research on social capital and civic engagement, we have decided to organize a symposium around the book, centered on three questions: 1) How is American Grace related to Putnam's earlier work, particularly Bowling Alone, and what are the implications of the continuities and/or discontinuities between these works? 2) What kind of a work of political science is American Grace, and how does it compare to other important recent work dealing with religion and politics in the United States? 3) What are the strengths and weaknesses of Putnam and Campbell's account of “how religion divides and unites us,” and what is the best way of thinking about the contemporary significance of religion and politics in the United States and about the ways that the religious landscape challenges U.S. politics and U.S. political science?
The evaluation of lifestyle interventions in the Netherlands
Authors: Rappange David R. , Brouwer Werner B. F. 
Published: March 2012

AbstractCurrent investments in preventive lifestyle interventions are relatively low, despite the significant impact of unhealthy behaviour on population health. This raises the question of whether the criteria used in reimbursement decisions about healthcare interventions put preventive interventions at a disadvantage. In this paper, we highlight the decision-making framework used in the Netherlands to delineate the basic benefits package. Important criteria in that framework are ‘necessity’ and ‘cost-effectiveness’. Several normative choices need to be made, and these choices can have an important impact on the evaluation of lifestyle interventions, especially when making these criteria operational and quantifiable. Moreover, the implementation of the decision-making framework may prove to be difficult for lifestyle interventions. Improvements of the decision-making framework in the Netherlands are required to guarantee sound evaluations of lifestyle interventions aimed at improving health.
Sensitivity of the shortwave radiative effect of dust on particle shape: Comparison of spheres and spheroids
Authors: Haapanala Päivi , Räisänen Petri , Kahnert Michael , Nousiainen Timo 
Published: March 2012

The sensitivity of direct shortwave radiative effects of dust (DRE) to assumed particle shape is investigated. Radiative transfer simulations are conducted using optical properties of either spheres, mass‐equivalent spheroids (mass‐conserving case), or (mass‐equivalent) spheroids whose number concentration is modified so that they have the same midvisible optical thickness (τ(545 nm)) as spheres (τ‐conserving case). The impact of particle shape on DRE is investigated for different dust particle effective radii, optical thickness of the dust cloud, solar zenith angle, and spectral surface albedo (ocean, grass, and desert). It is found that the influence of particle shape on the DRE is strongest over ocean. It also depends very strongly on the shape distribution of spheroids used, to a degree that the results for two distributions of spheroids may deviate more from each other than from those for spheres. Finally, the effects of nonsphericity largely depend on whether the mass‐ orτ‐conserving case is considered. For example, when using a shape distribution of spheroids recommended in a recent study for approximating the single‐scattering properties of dust, the DRE at the surface differs at most 5% from that from spherical particles in the mass‐conserving case. This stems from compensating nonsphericity effects on optical thickness, asymmetry parameter, and single‐scattering albedo. However, in theτ‐conserving case, the negative DRE at the surface can be up to 15% weaker for spheroids than spheres.
Peat accumulation in drained thermokarst lake basins in continuous, ice‐rich permafrost, northern Seward Peninsula, Alaska
Authors: Jones Miriam C. , Grosse Guido , Jones Benjamin M. , Walter Anthony Katey 
Published: March 2012

Thermokarst lakes and peat‐accumulating drained lake basins cover a substantial portion of Arctic lowland landscapes, yet the role of thermokarst lake drainage and ensuing peat formation in landscape‐scale carbon (C) budgets remains understudied. Here we use measurements of terrestrial peat thickness, bulk density, organic matter content, and basal radiocarbon age from permafrost cores, soil pits, and exposures in vegetated, drained lake basins to characterize regional lake drainage chronology, C accumulation rates, and the role of thermokarst‐lake cycling in carbon dynamics throughout the Holocene on the northern Seward Peninsula, Alaska. Most detectable lake drainage events occurred within the last 4,000 years with the highest drainage frequency during the medieval climate anomaly. Peat accumulation rates were highest in young (50–500 years) drained lake basins (35.2 g C m−2 yr−1) and decreased exponentially with time since drainage to 9 g C m−2 yr−1 in the oldest basins. Spatial analyses of terrestrial peat depth, basal peat radiocarbon ages, basin geomorphology, and satellite‐derived land surface properties (Normalized Difference Vegetation Index (NDVI); Minimum Noise Fraction (MNF)) from Landsat satellite data revealed significant relationships between peat thickness and mean basin NDVI or MNF. By upscaling observed relationships, we infer that drained thermokarst lake basins, covering 391 km2 (76%) of the 515 km2 study region, store 6.4–6.6 Tg organic C in drained lake basin terrestrial peat. Peat accumulation in drained lake basins likely serves to offset greenhouse gas release from thermokarst‐impacted landscapes and should be incorporated in landscape‐scale C budgets.
‘Coming out’ in therapy? Perceived risks and benefits of self‐disclosure of sexual orientation by gay and lesbian therapists to straight clients
Authors: Moore Jo , Jenkins Peter 
Published: March 2012

AbstractPrimary objective: The study's primary objective was to investigate the experiences of gay and lesbian therapists, when considering self‐disclosure of their sexual orientation to straight (i.e. heterosexual) clients. This qualitative study set out to ascertain the key factors gay therapists take into account when considering this decision and to explore the effects this self‐disclosure may have on themselves, their clients and their therapeutic relationships. Research design: The research design was based on the use of semi‐structured interviews, undertaken with a non‐random, purposive sample of counsellors and psychotherapists (n=8) in current practice. The rationale for this method was to aid exploration of therapists' experiences of the process of self‐disclosure. Methods and procedures: Inclusion criteria were for therapists who self‐identified as being gay or lesbian, and who were experienced in clinical work with straight clients. Transcribed data was analysed using inductive thematic analysis. Main outcomes and results: Some therapists reported being confident with self‐disclosure relating to their own sexual orientation. However, a common theme related to the reportedly high levels of anxiety and vulnerability, which therapists identified. Key themes emerged around: respondents' fears of client judgement; a need for therapist self‐protection; self‐awareness of the potential impact of their own fears and prejudices on the therapeutic relationship; and the potential relevance of internalised homophobia, as an overall constraining factor. Conclusions: Self‐disclosure of their sexual orientation to straight clients is constructed, in this initial survey, as being potentially problematic and risky for some gay therapists.
Accentuating the positive: The gendered identities of male problem‐drinkers, and the questions these pose for the counselling profession
Authors: Owen-Pugh Valerie , Allen Julie 
Published: March 2012

AbstractBackground: Few published studies have addressed the male experience of alcohol dependency. Aim: This paper seeks to address this deficiency and to highlight aspects of the male experience relevant to the counselling and psychotherapy profession. Methods: Six men in recovery from alcohol dependency participated in semi‐structured interviews. Findings: A grounded theory analysis elicited five core categories, Beginning to Drink, Paying for Gain with Pain, Reaching Rock Bottom, Struggling to Recover and Leading a New Life. Despite the pain they had experienced, participants' narratives were matter‐of‐fact and goal‐oriented. Low self‐esteem was expressed indirectly. Conclusions: These findings are consistent with socialisation models of alcoholism. Counsellors, and the wider profession, must be alert for the male under‐reporting of emotional distress.
Early immunologic correlates of HIV protection can be identified from computational analysis of complex multivariate T-cell flow cytometry assays
Authors: Aghaeepour Nima , Chattopadhyay Pratip K. , Ganesan Anuradha , O'Neill Kieran , Zare Habil , Jalali Adrin , Hoos Holger H. , Roederer Mario , Brinkman Ryan R. 
Published: March 2012

Abstract
Motivation: Polychromatic flow cytometry (PFC), has enormous power as a tool to dissect complex immune responses (such as those observed in HIV disease) at a single cell level. However, analysis tools are severely lacking. Although high-throughput systems allow rapid data collection from large cohorts, manual data analysis can take months. Moreover, identification of cell populations can be subjective and analysts rarely examine the entirety of the multidimensional dataset (focusing instead on a limited number of subsets, the biology of which has usually already been well-described). Thus, the value of PFC as a discovery tool is largely wasted.
Results: To address this problem, we developed a computational approach that automatically reveals all possible cell subsets. From tens of thousands of subsets, those that correlate strongly with clinical outcome are selected and grouped. Within each group, markers that have minimal relevance to the biological outcome are removed, thereby distilling the complex dataset into the simplest, most clinically relevant subsets. This allows complex information from PFC studies to be translated into clinical or resource-poor settings, where multiparametric analysis is less feasible. We demonstrate the utility of this approach in a large (n=466), retrospective, 14-parameter PFC study of early HIV infection, where we identify three T-cell subsets that strongly predict progression to AIDS (only one of which was identified by an initial manual analysis).
Availability: The ‘flowType: Phenotyping Multivariate PFC Assays’ package is available through Bioconductor. Additional documentation and examples are available at: www.terryfoxlab.ca/flowsite/flowType/
Supplementary information: Supplementary data are available at Bioinformatics online.
Contact: rbrinkman@bccrc.ca
Seabirds, set-nets, and conservation management: assessment of conflict potential and vulnerability of birds to bycatch in gillnets
Authors: Sonntag Nicole , Schwemmer Henriette , Fock Heino Ove , Bellebaum Jochen , Garthe Stefan 
Published: March 2012

AbstractSonntag, N., Schwemmer, H., Fock, H.O., Bellebaum, J., and Garthe, S. 2012. Seabirds, set-nets, and conservation management: assessment of conflict potential and vulnerability of birds to bycatch in gillnets. – ICES Journal of Marine Science, 69: 578–589. We addressed the issue of bird bycatch in set-nets in the southern Baltic Sea by (i) assessing the vulnerability of diving birds to drowning by developing a vulnerability index based on weighted bird abundance, (ii) providing information on set-net fishing activities, and (iii) using a spatial overlap approach to indicate the potential conflicts between diving birds and fishing activities. Birds and fisheries concentrated in the same areas. Vulnerability and potential conflict were highest during winter and spring in coastal waters and around shallow offshore grounds. Local bycatch studies validated the usefulness of our approach, which can provide a valuable tool for conservation purposes. Although the conflict analysis outlined the current extent of overlap between birds and fisheries, the vulnerability index indicated important areas and periods in terms of diving bird abundance, irrespective of fisheries, and enabled the development of appropriate conservation and management options. A suite of measures including temporal or spatial restrictions can be derived, despite a scarcity of real data for bycatch rates. This approach is particularly useful for assessing impacts that are difficult to monitor and where mortality cannot be properly addressed, as in artisanal gillnet fisheries. It is also generally applicable to any marine area or species worldwide.