bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of the Tgf-beta/smad Pathway in Tumor Radioresistance to Boron Neutron Capture Therapy (Bnct) in a Human Colon Carcinoma Cell Line
Authors: Pastini Antonella , Peralta Tomas , Nievas Susana , Carpano Marina , Curotto Paula , Pozzi Emiliano , Thorp Silvia , Rodriguez Carla , Oglio Romina , Perona Marina , Thomasz Lisa , Juvenal Guillermo , Rossini Luciano , Dagrosa Maria Alejandra 
Published: June 2024

Abstract

BNCT is a radiotherapy for different tumors as colorectal carcinoma (CRC). Although clinical outcomes show advantages of BNCT, tumor recurrence remains a common challenge. Previously we have described the double strand DNA damage response (DDR) produced by BNCT. TGF beta/SMAD pathway has been involved in maintaining genomic integrity. The aim of these studies was to evaluate the activation of the TGF-beta/SMAD pathway, its interaction with the DDR pathway and the possible use of LY2109761 (Ly), a specific inhibitor of TGF beta receptor, as a radiosensitizer for BNCT. Six groups were performed in a human colon adenocarcinoma (HT29) cell line: NCT (neutrons), BNCT (boronophenylalanine plus neutrons), Control and the same three groups with the addition of Ly. The results showed an activation of the TFG beta/Smad cascade with an increase in the genomic expression of TGF beta, Smad7 and Atr (p<0.001) at 2 hours post neutron treatments compared to the Control. A significant decrease in the expression of Tgf-beta receptor type I, Smad7 and Atr for BNCT plus Ly was observed. Furthermore, it was demonstrated a decrease in tumor survival as a function of the total absorbed physical dose for all the treatments, being significantly higher in the groups treated with Ly. On the other hand, a lower number of Ki67+ cells with the addition of Ly was found. Conclusion: The activation of the TGF-beta/SMAD pathway and its interaction with the DNA repair via through ATR transductor was demonstrated. LY2109761 could act as a radiosensitizer for BNCT.
Morphological profiling of T cells predicts clinical response to VLA-4-targeting natalizumab therapy in patients with multiple sclerosis
Authors: Chaves Beatriz , Silva Juan Carlo Santos e , Nakaya Helder , Almeida Matheus , Bucciarelli Florence , Lacouture Claire , Prunier Guilhèn , Kari Saniya , Astier Anne , Medeiros Marco , Silva João Hermínio da , Liblau Roland , Cotta-de-Almeida Vinícius , Dupré Loïc 
Published: June 2024

Abstract

Despite the efficacy of natalizumab in Multiple Sclerosis (MS) treatment, approximately 30% of patients do not respond favorably. Individual heterogeneity of T-cell response to VLA-4 natalizumab-mediated blockade may underlie disparities in treatment efficacy. Here, a high-content cell imaging (HCI) pipeline was implemented to profile the in vitro effects of natalizumab on VLA-4-stimulated leukocytes from MS patients prior to treatment. Unsupervised clustering of image data partially discriminated non-responder MS patients based on morphology, F-actin organization, and signaling-related features in CD8+ T cells. Treatment response was assessed through a Random Forest approach with predictive performance of 91% for a discovery cohort and 70% for a validation cohort. Unfavorable treatment response was associated with the inefficacy of natalizumab to impair the ability of pretreated CD8+ T cells to spread over VCAM-1. Our study unveils that CD8+ T cells from individual MS patients display heterogeneous susceptibility to natalizumab in vitro and highlights the potential of HCI-based pretreatment monitoring to assist individualized treatment prescription.
Response of Root System to Sequential Salinity Conditions in Salinity-Tolerant/Sensitive Soybean Varieties
Authors: Rui Qiuzhi , Tajima Ryosuke , Ye Rongling , Kameoka Emi , Xu Donghe , Homma Koki 
Published: June 2024

Abstract

The root is one of the vital organs of soybean for combating salt stress. Previous studies have indicated that the salt-tolerant gene Ncl reduces the chloride and sodium ion content in stems and leaves, and it can significantly increase yield under saline conditions. The expression of theNcl gene is the highest in soybean roots. To further clarify the response of salt-tolerant soybean roots to salt stress, this study conducted hydroponic experiments on three groups of near-isogenic lines (NILs) with different salt tolerance levels, observing the effect of the gene Ncl on soybean root growth under ten salt concentrations. The results indicate that under salt stress, the Ncl gene not only maintains the biomass of aboveground parts but also sustains root biomass. However, the effect of the Ncl gene on root length varies among different NILs. Additionally, under salt stress, the Ncl gene improves morphological characteristics of the root, especially lateral roots number and root surface area. Concerning xylem sap, no significant difference was observed between salt-tolerant and salt-sensitive varieties, indicating that the Ncl gene does not affect osmotic water absorption under salt stress. However, the hydraulic resistance of salt-tolerant varieties is significantly higher than that of salt-sensitive varieties. Finally, interactions between salt concentration and salt tolerance were observed in root dry weight and root volume, suggesting the Ncl gene makes a trade-off between root growth and soybean salt tolerance.
Failure Of Second Line ART Demands Third -Line Options For HIV-Infected Patients In Telangana, India
Authors: G Krishna , Pandharpurkar Deepak , Mallikarjun P. 
Published: June 2024

Back ground: The research was carried out with the aim of detecting instances of second-line antiretroviral therapy (ART) failure and exploring the potential of third-line ART treatment for individuals with HIV, incorporating the utilization of HIV viral load (VL) testing. Beginning in 2016-17, individuals living with HIV are directed to government ART centers and ART plus centers for treatment. Patients who have experienced treatment failure with second-line therapy and have been exposed to multiple NRTIs, in accordance with the Indian National ART Guidelines, are referred to the SACEP Clinic for further evaluation. If deemed eligible according to the Indian National 3rd Line ART Guidelines, these patients are enrolled and followed up for the initiation of 3rd Line ART at our center. Throughout the duration of the study, a collective of 55 individuals diagnosed with Human Immunodeficiency Virus (HIV) were identified as potentially experiencing a lack of efficacy with regards to their secondary line antiretroviral therapy (ART). Two patients in the cohort were diagnosed with both HIV and multi drug resistant tuberculosis (MDR-TB).
Method: Fifty-four and a half percent of the patient sample was classified as being in WHO stage I, while 32.8% were categorized as being in stage II, and the remaining 12.7% were identified as being in WHO stage IV. Fifty-five patients who were suspected of experiencing second-line treatment failure were transitioned to third-line antiretroviral therapy.
Results: All 55 patients underwent VL follow-up, with 53 of them attaining viral suppression within a median period of 6 months. However, two patients were unable to achieve viral suppression.
Conclusion: However, this study describes HIV-positive patients who need third-line ART due to probable second-line ART failure.
Correlation Of Albumin Levels With Glucose Levels in Hypoglycemic Patients After The Use Of Insulin Therapy
Authors: Fajari Nanang Miftah , Kholifah Rika , Rosida Azma 
Published: June 2024

Background: Most of the incidence of hypoglycemia in hospital (11.29%) is caused by administering insulin therapy as a therapeutic modality in diabetes mellitus patients during hospitalization. The bond between albumin and insulin using the help of fatty acids makes insulin control the body's glycemic levels.
Aim: The purpose of this study was to determine and analyze the correlation between albumin levels and glucose levels in hypoglycemia patients after the administration of insulin therapy at the Ratu Zalecha General Hospital.
Methods: This study was an analytic observational study with a cross-sectional approach. A total of 32 samples were obtained by purposive sampling
Results: The patient's mean glucose level was 48.95 ± 11.5 mg/dL and the patient's mean albumin level was 2.4 ± 0.6 g/dL. Bivariate analysis using Pearson's correlation test showed a correlation between albumin levels and glucose levels (p = 0.045 <0.05; r = 0.356).
Conclusion: The conclusion is that there is a significant correlation between albumin levels and blood glucose levels, the direction of the positive correlation with the strength of the weak correlation in the endocrine internal medicine ward of the Ratu Zalecha Martapura Hospital.
Internet Use and Higher-Level Functional Capacity Decline Suppression in Japanese Older Adults With Low Education: JAGES 2016-2019 Longitudinal Study
Authors: Tajika Atsuko , Nakagomi Atsushi , Miyaguni Yasuhiro , Koga Chie , Kondo Katsunori , Ojima Toshiyuki 
Published: June 2024

Background
Higher-level functional capacity (HLFC) is crucial for the independent living of older adults. While internet use positively impacts the health of older adults, its effect on HLFC and how this effect varies with educational attainment remains uncertain.

Objective
This longitudinal study aimed to investigate whether internet use could mitigate the risk of HLFC decline and if this benefit extends to older adults with lower levels of education.

Methods
The data were sourced from the Japan Gerontological Evaluation Study (JAGES), encompassing 8050 community-dwelling adults aged 65 years and older from 2016 to 2019. The study focused on those who remained self-sufficient from 2016 to 2019, identifying participants with independent HLFC in 2016. The Tokyo Metropolitan Institute of Gerontology Index of Competence defined HLFC operationally, consisting of 3 subscales, namely instrumental activities of daily living, intellectual activity, and social role. The primary variable was the frequency of internet use in 2016; participants who reported using the internet were classified as internet users, while those who answered “No” were identified as nonusers. The study compared the effects of internet use on HLFC decline across educational levels of ≤9 years, 10-12 years, and ≥13 years using Poisson regression analysis adjusted for robust SE to calculate the risk ratio (RR) and 95% CI for HLFC decline in 2019.

Results
After adjusting for demographic and health condition risk factors, internet use was significantly linked to a decreased risk of HLFC decline in older adults over 3 years, including those with lower educational levels. Internet users with ≤9 years of educational attainment experienced a suppressed decline in the total score (RR 0.57, 95% CI 0.43-0.76; P<.001); instrumental activities of daily living (RR 0.58, 95% CI 0.38-0.91; P=.02), intellectual activity (RR 0.60, 95% CI 0.41-0.89; P=.01), and social role (RR 0.74, 95% CI 0.56-0.97; P=.03) compared with nonusers. Participants with 10-12 years of education showed suppression rates of 0.78 (95% CI 0.63-0.98; P=.03), 0.59 (95% CI 0.39-0.90; P=.01), 0.91 (95% CI 0.63-1.31; P=.61), and 0.82 (95% CI 0.68-1.00; P=.05), respectively, and those with ≥13 years displayed suppression rates of 0.65 (95% CI 0.51-0.85; P=.001), 0.55 (95% CI 0.36-0.83; P=.01), 0.64 (95% CI 0.37-1.10; P=.11), and 0.83 (95% CI 0.64-1.08; P=.17), respectively.

Conclusions
These findings indicate that internet use supports the maintenance of HLFC independence in older adults with higher education and those with lower educational levels. Encouraging internet use among older adults with lower levels of education through future policies could help narrow functional health disparities associated with educational attainment.
The curative effect of ethanolic extract of Moringa oleifera leaf in an animal model of liver fibrosis
Authors: Supriono  , Putri Dinda Amalia Eka , Priyanto Tia Rahmi , Sholihah Al Imroatus , Larasati Putu Anissa , Nabila Salsala Sifa , Hartaya Arsy Hanandya , Budiarto Andika Agus , Fachrureza Mochamad , Wibowo Bogi Pratomo , Mustika Syifa 
Published: June 2024

Studies about the prevention effect of Moringa oleifera on liver fibrosis has been reported. However, its curative effect has not been reported, yet. This study was conducted to evaluate the curative effect of M. oleifera leaf extract on liver fibrosis. It was a laboratory experimental study with a post-test-only control group design. Rats were divided into 5 groups i.e. normal control which received intraperitoneally injections of 1 mL/kg BW of 0.9% NaCl solution twice a wk for 11 wk. Liver fibrosis control which received intraperitoneally injections of 1 mL/kg BW of 10% CCl4 solution twice a wk for 11 wk. Three M. oleifera treatment group which received intraperitoneally injections of 1 mL/kg BW of 10% CCl4 solution twice a wk for 11 wk continued by M. oleifera leaf ethanolic extract at dose of 600 mg/kg BW daily for 3 (MO3), 6 (MO6), and 10 (MO10) wk, respectively. The liver fibrosis level was assessed based on the METAVIR score. Histopathological analysis of liver tissues demonstrated that the 11-week CCl4 induction successfully resulted in liver fibrosis in rats (F3 and F4). The administration of M. oleifera leaf ethanolic extract decreased METAVIR scores ranged from F3 to F1. The optimal reduction of the METAVIR scores (F1) was observed in MO3 group after 6 wk administration (p<0.05). It was indicated that M. oleifera leaf ethanolic extract ameliorated liver fibrosis. In conclusion, M. oleifera leaf ethanolic extract has a curative effect against liver fibrosis.
(Re)Pensando as práticas de leitura na escola: a emancipação por meio do texto literário
Authors: Garcia Eliane Dolens Almeida , Pereira Marcos Aparecido 
Published: June 2024

O texto literário, quando trabalhado sob uma perspectiva dialógica, assume um papel fundamental na formação de um sujeito cidadão com competência criativa, consciente social e culturalmente, possibilitando ao aluno uma maneira mais crítica de repensar a sociedade e o mundo. Nossa pesquisa busca analisar como se dá a recepção de textos da literatura de expressão Mato-grossense numa turma de 9º ano do Ensino Fundamental II, enfatizando as possibilidades de uso do texto literário e sua contribuição na formação de leitores literários, bem como discutir a potencialidade dos textos literários contidos na Revista Literária Pixé para a continuidade do processo de leitura com viés emancipatório e postura mais consciente com relação a vida e a literatura, num constante enriquecimento cultural e social. O estudo proposto pauta-se na pesquisa qualitativa, em relação aos procedimentos trabalhamos com o estudo de caso. Enquanto base da construção teórica, foram tomados como referência autores e filósofos como Mikhail M. Bakhtin (1986), Bordini (1993); Aguiar (1993), Bourdieu (1989), Candido (1972) e Freire (2003).
O Ensino de Termodinâmica e as suas relações com questões ambientais: uma revisão sistemática de literatura
Authors: Cardoso João Michels , Raupp Daniele Trajano 
Published: June 2024

A natureza abstrata e complexa da Termodinâmica demanda abordagens variadas e contextualização com exemplos práticos para superar dificuldades de aprendizagem. Nesse sentido, apresentamos uma revisão de literatura, realizada no Portal de Periódicos CAPES, objetivando compreender metodologias utilizadas, materiais didáticos e contextos de pesquisa no ensino de Termodinâmica. Ademais, investigamos a relação com questões ambientais, em virtude do papel significativo dessa temática, com tópicos como eficiência energética, mudanças climáticas e impacto das ações antrópicas no ambiente. Aplicando os critérios de seleção, dez manuscritos foram examinados, utilizando Análise de Conteúdo de Bardin e alocados nas categorias: História da Ciência, Papel da Experimentação, Investigações de Conceitos Espontâneos e Científicos, Implicações para o Ensino de Ciências e Materiais Didáticos. Somente um estudo apresentou relação com questões ambientais, o que reflete uma visão simplista e reducionista, utilizando apenas exemplos das máquinas térmicas, sem considerar a relevância da abordagem dos conceitos de termodinâmica com as questões ambientais atuais.
PENENTUAN HARI RAYA ISLAM DAN IMPLIKASINYA TERHADAP PERSATUAN UMAT
Authors: Ningsi Siska wati 
Published: June 2024

Skripsi ini berjudul Penentuan Hari Raya Islam dan Implikasinya Terhadap Persatuan Umat. Perbedaan waktu dalam pelaksanaan awal bulan Ramadan dan hari raya umat islam mewarnai umat muslim di Indonesia. Dan perbedaan sering terjadi dari tahun ke tahun, berbedaan tersebut bukan hanya terjadi penentuan pemerintah, melelui dua ormas besar yang ada di Indonesia seperti NU dan Muhammadiyah.Tujuan dari penelitian ini adalah untuk mengetahui dan menyatukan pendapat pemerintah dan para ormas dalam penentuan hari raya islam baik hari raya Idul Fitri maupun Idul Adha agar tidak terjadi perpecahan dan saling menyalahkan antara umat muslim. Dan untuk itu pemerintah harus mengumpulkan dua organisasi tersebut, serta mengikutkan para kolompok-kolompok yang ahli dalam hisab dan rukyah. Selanjutnya pendapat-pendapat di satukan dan di sepakati oleh pemerintah untuk mengumumkan pada sidang isbat yang dilaksanakan oleh kementrian agama.Pada penelitian ini ada beberapa kesimpulan yang ada pertama, terjadinya berbedaan pemahaman dan metode yang digunakan dua ormas yang ada di Indonesia. Kedua metode yang di gunakan untuk penentuan hari raya islam adalah metode hisab dan rukyah, dan pemerintah bisa menggabukan kedua metode tersebut yang di namakan imkanur rukyah. Ketiga, umat muslim harus saling menghargai walaupun ada perbedaan pada penentuan hari raya islam. Implikasi dari penelitian ini adalah, pertama, diharapkan kepada masyarakat agar tetap mengikuti penetapan pemerintah tentang hari raya umat Islam. Dan saling menghargai satu berbadaan pendapat tentang pelaksanaan hari raya Idul Fitri dan Idul Adha. Kedua, pemerintah sangat berperang penting dalam hal penetapan hari raya umat Islam agar masyarakat tidak lagi mengalami perpecahan masalah penetapan di laksanakannya hari raya umat Islam.
ANALISIS PENANGGALAN DALAM TRADISI REKENG-REKENG DI KELURAHAN GANTARANG KECAMATAN TINGGMONCONG KABUPATEN GOWA PERSFEKTIF ILMU FALAK
Authors: Ashari Yusran  , Irfan  , Sohra  
Published: June 2024

Penelitian ini membahas tentang Sistem Penanggalan Dalam Tradisi Rekeng-Rekeng di Kelurahan Gantarang Kecamatan Tinggimoncong Kabupaten Gowa Perspektif Ilmu Falak. Pendapat masyarakat di Kelurahan Gantarang terhadap sistem penanggalan dalam tradisi rekeng-rekeng sudah melekat dalam kehidupan masyarakatnya karena hal tersebut sudah lama digunakan dan menjadi tradisi turun temurun dengan cara melihat posisi terbitnya bulan untuk melihat waktu yang baik dalam melakukan suatu aktivitas. Sebagian masyarakat dalam menjalankan tradisi rekeng-rekeng menggunakan pedoman naskah dan juga bertanya kepada orang yang ahli dalam rekeng-rekeng. Sistem penanggalan dalam tradisi rekeng-rekeng perspektif Ilmu Falak menggunakan sistem peredaran bulan atau penanggalan Hijriyah. Hanya saja penanggalan dalam tradisi rekeng-rekeng ini bersifat khusus. Tradisi rekeng- rekeng memiliki fungsi sebagai kebiasaan mencari hari baik dalam memulai aktivitas. Dalam penentuan hari baik dan hari buruk didasarkan pada kejadian yang dialami masyarakat Makassar terlebih khusus di Kelurahan Gantarang memiliki keyakinan bahwa proses alam yang dalam hal ini pergantian bulan mempunyai makna mitologi yang kemudian akan mempengaruhi semua aktivitas manusia bahkan dipercaya dan dijadikan sebagai pedoman dalam memulai aktivitas.
How can we achieve more accurate reporting of average dog lifespan?
Authors: Sexton Courtney L. , Ruple Audrey 
Published: June 2024

Abstract
Despite major advances in our understanding of dogs as a biological system (including genetics/epigenetics, physiology, cognition, and behavior), the veterinary field lacks consensus around a critical piece of information: namely, the average lifespan/life expectancy of a domestic dog. This deficiency is due in part to unavailable and/or inconsistent collection of dog mortality data. In an effort to review historical and current reports of dog lifespan to determine whether the domestic dog’s lifespan has changed over time, we found that incongruous data were prohibitive to conducting a formal meta-analysis of dog lifespan reports. However, in examining several different kinds of dog aging and mortality studies covering a span of about 40 years (1981 to 2023), it seems apparent that the median lifespan of domesticated dogs has not recently decreased, as has been reported in the popular press, but rather has increased steadily over that time frame. Still, assessing the validity of these numbers is a challenge, as methodology, cohort, and covariates (such as weight, breed, etc) vary from study to study. We therefore recommend the adoption of a comprehensive, standardized method for reporting and recording dog mortality so that a more accurate understanding of dogs’ average lifespan can be obtained in the future.
Summary of Regulatory Methods and Procedures for Determination of Harmful and Potentially Harmful Components in Tobacco Smoke
Authors: Docheva Margarita H. , Kirkova Desislava M. , Stoyanova Liliya S. 
Published: June 2024

Summary
Tobacco smoke consists of over 5000 chemical components, most of them are carcinogenic, respiratory toxicants, cardiovascular toxicants, addictive, etc. In 2006 the Working Group on Tobacco Control identified substances in tobacco smoke, which are harmful and potentially harmful for human health (Priority List) and encouraged the development of methods for the analysis of these components. This review focuses on the different methods of evaluation of harmful and potentially harmful components in tobacco smoke used by the International Agency for Research on Cancer (IARC) and the U.S. Food and Drug Administration (FDA) and furthermore on the official methods proposed by the World Health Organization (WHO) and the Cooperation Center for Scientific Research Relative to Tobacco (CORESTA) for analysing these constituents. The various methods for the determination of the substances in tobacco smoke included in the Priority List are compared. In addition, the different evaluation of components from the Priority List is presented. The authors hope that this review will acquaint readers with the harmful components in tobacco smoke. Also, that it will help accredited or scientific laboratories to compare the different methods and to choose appropriate methods depending on their laboratory equipment and laboratory chemicals available.