bluuebunny/crossref_metadata_2025_split huggingface dataset:
International field testing of the reliability and validity of the EORTC QLQ‐BM22 module to assess health‐related quality of life in patients with bone metastases
Authors: Chow Edward , Nguyen Janet , Zhang Liying , Tseng Ling‐Ming , Hou Ming‐Feng , Fairchild Alysa , Vassiliou Vassilios , Jesus‐Garcia Reynaldo , Alm El‐Din Mohamed A. , Kumar Aswin , Forges Fabien , Chie Wei‐Chu , Bottomley Andrew ,   On behalf of the European Organization for Research Treatment of Cancer Quality of Life Group
Published: August 2011

AbstractBACKGROUND:The objective of this international field study was to test the reliability, validity, and responsiveness of the European Organization for Research and Treatment of Cancer (EORTC) QLQ‐BM22 module to assess health‐related quality of life (HRQOL) in patients with bone metastases.METHODS:Patients undergoing a variety of bone metastases‐specific treatments were accrued. The QLQ‐BM22 was administered with the QLQ‐C30 at baseline and at 1 follow‐up time point internationally. A debriefing questionnaire was administered to determine patient acceptability and understanding.RESULTS:Large‐scale field testing of the QLQ‐BM22 in addition to the QLQ‐C30 took place in 7 countries: Brazil, Canada, Cyprus, Egypt, France, India, and Taiwan. A total of 400 patients participated. Multitrait scaling analyses confirmed 4 scales in the 22‐item module. The scales were able to discriminate between clinically distinct patient groups, such as between those with a poor and those with a better performance status. The QLQ‐BM22 was well received in all 7 countries, and the majority of patients did not recommend any significant changes from the module in its current form.CONCLUSIONS:The final QLQ‐BM22 module contains 22 items and 4 scales assessing Painful Sites, Painful Characteristics, Functional Interference, and Psychosocial Aspects. Results confirmed the validity, reliability, cross‐cultural applicability, and sensitivity of the 22‐item EORTC QLQ‐BM22. It is therefore recommended that the QLQ‐BM22 be used in addition to the QLQ‐C30 in clinical trials to assess HRQOL in patients with bone metastases. Cancer 2012;. © 2011 American Cancer Society.
Impact of low estrogen/progesterone receptor expression on survival outcomes in breast cancers previously classified as triple negative breast cancers
Authors: Raghav Kanwal P. S. , Hernandez‐Aya Leonel F. , Lei Xiudong , Chavez‐MacGregor Marianan , Meric‐Bernstam Funda , Buchholz Thomas A. , Sahin Aysegul , Do Kim‐Anh , Hortobagyi Gabriel N. , Gonzalez‐Angulo Ana M. 
Published: August 2011

AbstractPURPOSE:To evaluate the impact of low estrogen/progesterone receptor (ER/PR) expression and effect of endocrine therapy on survival outcomes in human epidermal growth factor receptor 2 (HER2)‐negative tumors with ER/PR <10%, previously labeled as triple negative.METHODS:In a retrospective review, 1257 patients were categorized according their ER/PR percentages into 3 groups, ER/PR <1% (group A), ER/PR 1% to 5% (group B), and ER/PR 6% to 10% (group C). Kaplan‐Meier product limit method was used to estimate survival outcomes. Cox proportional hazards models was used to adjust for patient and tumor characteristics.RESULTSGroups A, B, and C had 897 (71.4%), 241 (19.2%), and 119 (9.4%) patients, respectively. After a median follow‐up of 40 months there was no significant difference in 3‐year recurrence‐free survival (RFS): 64%, 67%, and 77% (P = .34) or overall survival (OS): 79%, 81%, and 88% (P = .33) for groups A, B, and C, respectively. ER/PR expression was not an independent predictor for RFS (hazard ratio [HR], 1.10; 95% confidence interval [CI], 0.86‐1.39; P = .46 for group B, and HR, 0.96; 95% CI, 0.66‐1.38; P = .81 for group C, compared with group A), or OS (HR, 1.11; 95% CI, 0.84‐1.46; P = .46 for group B, and HR, 0.94; 95% CI, 0.63‐1.42; P = .78 for group C, compared with group A). Endocrine therapy had no impact on survival outcomes (RFS: P = .10; OS: P = .45) among groups.CONCLUSIONS:In this cohort, a low ER/PR level (1%‐5%) does not appear to have any significant impact on survival outcomes. There was a tendency for survival advantages in the ER/PR 6% to 10% is seen. Benefit of endocrine therapy in these patients is unclear. Cancer 2011;. © 2011 American Cancer Society.
Synthesis of Mono‐Functionalized Core‐Modified Expanded Porphyrin Building Blocks and Covalently Linked Expanded Porphyrin Dyads
Authors: Pareek Yogita , Ravikanth M. 
Published: August 2011

AbstractA series of mono‐functionalized core‐modified expanded porphyrin building blocks such as thiasapphyrin, thiarubyrin, oxasmaragdyrin, and BF2–oxasmaragdyrin have been synthesized by simple condensation of readily available precursors. The mono‐functionalized core‐modified expanded porphyrin building blocks were used to synthesize the first three examples of covalently linked diphenylethyne‐bridged dyads containing two different expanded porphyrin macrocycles, namely thaisapphyrin–BF2–oxasmaragdyrin, thiarubyrin–BF2–oxasmaragdyrin, and thiasapphyrin–thiarubyrin, by coupling appropriate mono‐functionalized expanded porphyrin building blocks under mild Pd0 coupling reaction conditions. The three dyads were freely soluble in common organic solvents and characterized by MS, NMR, absorption, electrochemical, and fluorescence techniques. The NMR, absorption, and electrochemical studies indicated that the two macrocycles in the dyads interact weakly with each other and maintain their independent characteristic features. The steady‐state fluorescence studies of the dyads showed that the thiasapphyrin and thiarubyrin units are nonfluorescent but fluorescence was observed from the BF2–oxasmaragdyrin unit. However, the quantum yield of the BF2–oxasmaragdyrin unit in the dyads was less than that of monomeric BF2–oxasmaragdyrin because of an enhancement of nonradiative decay channels operating in the dyads. The potential use of two of the three dyads containing the BF2–smaragdyrin subunit as fluorescent sensors for anions was explored. The studies showed that the binding of the anion at the protonated sapphyrin and rubyrin sites in the respective dyads can be followed by the clear changes in the fluorescence band of the BF2–oxasmaragdyrin unit, which indicates that these dyads can be used as fluorescent anion sensors.
Reductive Dealkylation of Anisole and Phenetole: Towards Practical Lignin Conversion
Authors: Strassberger Zea , Tanase Stefania , Rothenberg Gadi 
Published: August 2011

AbstractWe present and develop alternative catalysts for biomass conversion and specifically lignin conversion into aromatics. Unlike the conventional CoMo and NiMo formulations, our catalysts can convert low‐sulfur feedstocks. A set of five magnesia–alumina mixed oxides were screened in the hydrodealkylation of alkyl phenyl ethers as lignin model compounds. The typical selectivity to phenol is 30–75 %. Interestingly, we saw that the more basic the catalyst, the higher the selectivity for phenol. The results concur with the formation of phenoxide (PhO–) and RH3+ fragments on the catalyst surface. These can then react with H+ and H– species formed by the hydrogen dissociation on the MgO surface, giving phenol and hydrocarbons. We conclude that magnesia–alumina mixed oxides are attractive candidates for catalyzing lignin breakdown. These catalysts are highly stable, inexpensive, and readily available.
Analytical Determination and Electrochemical Characterization of the Oxazolidinone Antibiotic Linezolid
Authors: Merli Daniele , Pretali Luca , Fasani Elisa , Albini Angelo , Profumo Antonella 
Published: August 2011

AbstractLinezolid belongs to a class of antibiotics known as oxazolidinones. It is receiving great attention due to its activity toward multidrugs resistant bacteria, and simple and cheap methods for the analysis of this compound in pharmaceutical preparations are required. In this work, the electrochemistry of the antibiotic linezolid was investigated and a differential pulse voltammetric method based on the oxidation of the oxazolidinonic compound at a glassy carbon electrode has been developed and optimized for its determination; LOD of 50 µg L−1 and a linearity up to 200 mg L−1 were reported. The electrochemical process was studied by using different electrochemical techniques and confirmed by HPLC‐MS/MS.
An Investigation of Goodman's Addictive Disorder Criteria in Eating Disorders
Authors: Speranza Mario , Revah‐Levy Anne , Giquel Ludovic , Loas Gwenolé , Venisse Jean‐Luc , Jeammet Philippe , Corcos Maurice 
Published: August 2011

AbstractThe aim of this study was to examine how far Goodman's addictive disorder criteria were met by individuals with eating disorders according to subtypes. The study provided a cross‐sectional comparison among three samples of eating disorders [restricting anorexia nervosa (R‐AN), N = 68; purging anorexia nervosa (P‐AN), N = 42; and bulimia nervosa (BN), N = 66], a sample of substance‐related disorders (SRDs, N = 48) and a sample of matched controls (N = 201). Diagnosis of addictive disorder was made following Goodman's criteria. Addictive personality traits were assessed with the Addiction Potential Scale of the Minnesota Multiphasic Personality Inventory—2 and with the Zuckerman's Sensation Seeking Scale. Results showed that individuals with BN met Goodman's addictive disorder criteria in the same proportion as drug‐addicted individuals (65% vs 60%, p = NS). They both showed higher rates than R‐AN individuals (35%; R‐AN versus BN: F = 11.9, p < 0.001 and R‐AN versus SRD: F = 7.16, p < 0.01). Although BN and SRD showed higher rates of addictive disorders compared with P‐AN, differences were not significant. Scores on the Sensation Seeking and on the Addictive Potential scales paralleled the distribution of addictive disorders, with individuals with BN and with P‐AN showing higher levels than individuals with R‐AN. Results showed that a subgroup of individuals with an eating disorder experiences their disorder as an addiction and may deserve specific therapeutic attention. Copyright © 2011 John Wiley & Sons, Ltd and Eating Disorders Association.
Simulation of self‐propelled anguilliform swimming by local domain‐free discretization method
Authors: Zhou C.H. , Shu C. 
Published: August 2011

SUMMARYThe local domain‐free discretization method is extended in this work to simulate fluid–structure interaction problems, the class of which is exemplified by the self‐propelled anguilliform swimming of deforming bodies in a fluid medium. Given the deformation of the fish body in its own reference frame, the translational and rotational motions of the body governed by Newton's Law are solved together with the surrounding flow field governed by Navier–Stokes equations. When the body is deforming and moving, no mesh regeneration is required in the computation. The loose coupling strategy is employed to simulate the fluid–structure interaction involved in the self‐propelled swimming. The local domain‐free discretization method and an efficient algorithm for classifying the Eulerian mesh points are described in brief. To validate the fluid–structure interaction solver, we simulate the ‘lock‐in’ phenomena associated with the vortex‐induced vibrations of an elastically mounted cylinder. Finally, we demonstrate applications of the method to two‐dimensional and three‐dimensional anguilliform‐swimming fish. The kinematics and dynamics associated with the center of mass are shown and the rotational movement is also presented via the angular position of the body axis. The wake structure is visualized in terms of vorticity contours. All the obtained numerical results show good agreement with available data in the literature. Copyright © 2011 John Wiley & Sons, Ltd.
Characterization of canine osteosarcoma by array comparative genomic hybridization and RT‐qPCR: Signatures of genomic imbalance in canine osteosarcoma parallel the human counterpart
Authors: Angstadt Andrea Y. , Motsinger‐Reif Alison , Thomas Rachael , Kisseberth William C. , Guillermo Couto C. , Duval Dawn L. , Nielsen Dahlia M. , Modiano Jaime F. , Breen Matthew 
Published: August 2011

AbstractOsteosarcoma (OS) is the most commonly diagnosed malignant bone tumor in humans and dogs, characterized in both species by extremely complex karyotypes exhibiting high frequencies of genomic imbalance. Evaluation of genomic signatures in human OS using array comparative genomic hybridization (aCGH) has assisted in uncovering genetic mechanisms that result in disease phenotype. Previous low‐resolution (10–20 Mb) aCGH analysis of canine OS identified a wide range of recurrent DNA copy number aberrations, indicating extensive genomic instability. In this study, we profiled 123 canine OS tumors by 1 Mb‐resolution aCGH to generate a dataset for direct comparison with current data for human OS, concluding that several high frequency aberrations in canine and human OS are orthologous. To ensure complete coverage of gene annotation, we identified the human refseq genes that map to these orthologous aberrant dog regions and found several candidate genes warranting evaluation for OS involvement. Specifically, subsequenct FISH and qRT‐PCR analysis of RUNX2, TUSC3, and PTEN indicated that expression levels correlated with genomic copy number status, showcasing RUNX2 as an OS associated gene and TUSC3 as a possible tumor suppressor candidate. Together these data demonstrate the ability of genomic comparative oncology to identify genetic abberations which may be important for OS progression. Large scale screening of genomic imbalance in canine OS further validates the use of the dog as a suitable model for human cancers, supporting the idea that dysregulation discovered in canine cancers will provide an avenue for complementary study in human counterparts. © 2011 Wiley‐Liss, Inc.
Establishment of a novel MALT lymphoma cell line, ma‐1, from a patient with t(14;18)(q32;q21)‐positive Helicobacter Pylori‐Independent Gastric MALT Lymphoma
Authors: Kuo Sung‐Hsin , Weng Wen‐Hui , Chen Zi‐Hua , Hsu Ping‐Ning , Wu Ming‐Shiang , Lin Chung‐Wu , Jeng Huei‐Jiuan , Yeh Kun‐Huei , Tsai Hui‐Jen , Chen Li‐Tzong , Cheng Ann‐Lii 
Published: August 2011

AbstractAlthough t(11;18)(q21;q21), t(1;14)(p22;q32), and a few other genetic mutations are specific markers for the Helicobacter pylori (HP)‐independent status of gastric mucosa‐associated lymphoid tissue (MALT) lymphoma, the molecular mechanisms responsible for HP‐independence of gastric MALT lymphoma without such translocations and mutations remain uncharacterized. In the present study, we describe the establishment and characterization of a novel MALT lymphoma cell line, MA‐1, which was derived from a gastric MALT lymphoma which was negative for both t(11;18)(q21;q21) and t(1;14)(p22;q32); the patient had failed HP eradication therapy and chemotherapy. The cell morphology and the immunophenotype of this cell line were similar to that of the original gastric MALT lymphoma. Comparative genomic hybridization analysis showed no significant gene copy number changes. Spectral karyotyping displayed a near‐diploid chromosome content (48 < 2n>XY), with at least 13 chromosome structural abnormalities. Furthermore, fluorescence in situ hybridization analyses disclosed the existence of three sub‐clones, characterized by t(14;18)(q32;q21)/IGH‐BCL2, t(14;18)(q32;q21)/IGH‐MALT1, and the presence of both chromosomal translocations in the same cell, respectively; whereas amplification of the genes CRAD9, TRAF2, and BCL10 were not found. In conclusion, we have established the first human gastric MALT lymphoma cell line, which is characterized by unusual and complex chromosome translocations and will be useful to explore further the molecular mechanisms of HP‐independence in gastric MALT lymphoma. © 2011 Wiley‐Liss,Inc.
Detection of the first gross CDC73 germline deletion in an HPT‐JT syndrome family
Authors: Cascón Alberto , Huarte‐Mendicoa Carlos Vázquez , Javier Leandro‐García L. , Letón Rocío , Suela Javier , Santana Alfredo , Costa Mauro Boronat , Comino‐Méndez Iñaki , Landa Iñigo , Sánchez Lydia , Rodríguez‐Antona Cristina , Cigudosa Juan C. , Robledo Mercedes 
Published: August 2011

AbstractHereditary primary hyperparathyroidism (HPT) may develop as a solitary endocrinopathy (FIHP) or as part of multiple endocrine neoplasia Type 1, multiple endocrine neoplasia Type 2A, or hereditary HPT‐jaw tumor syndrome. Inactivating germline mutations of the tumor suppressor gene CDC73 account for 14 and 50% of all FIHP and HPT‐JT patients, respectively, and have also been found in almost 20% of apparently sporadic parathyroid carcinoma patients. Although more than 60 independent germline mutations have been described, to date no rearrangement affecting the CDC73 locus has been identified. By means of multiplex‐PCR we found a large germline deletion affecting the whole gene in a two‐generation HPT‐JT family. Subsequently array‐CGH and specific PCR analysis determined that the mutation spanned ∼ 547 kb, and included four additional genes:
TROVE2, GLRX2, B3GALT2, and UCHL5. Although no clear mutation‐specific phenotype was found associated to the presence of the mutation, further studies are needed to assess whether the loss of the neighboring genes could modify the phenotype of carriers. There was complete absence of nuclear staining in the two HPT‐JT‐related tumors available. The finding of the first rearrangement affecting the CDC73 gene warrants screening for this tumor suppressor gene inactivation mechanism not only in high‐risk CDC73 point mutation‐negative HPT‐JT families, but also in FIHP patients. © 2011 Wiley‐Liss, Inc.
DISCOUNTING HEALTH AND COST‐EFFECTIVENESS ANALYSIS: A RESPONSE TO NORD
Authors: Hammitt James K. 
Published: August 2011

SUMMARYNord (2011) criticizes standard arguments which assert that consistency requires that future health benefits must be discounted at the same rate as future costs in cost‐effectiveness analysis (CEA). He suggests these arguments are misguided because they require transitivity of preferences across decision contexts and that it can be appropriate to discount health at different rates depending on the programs to be compared. I claim that rejecting transitivity is unwarranted and would sharply diminish the utility of CEA. Factors that tempt Nord to reject consistency can be accommodated by recognizing that CEA does not perfectly mimic normative social preferences because it omits factors (like distribution of health in a population) that can be normatively significant. A better approach is to maintain consistency in application of CEA but authorize decision makers to depart from rankings implied by CEA when justified and to explain which specific factors justify the decision. Finally, the assertion that health must be discounted at the same rate as costs requires the additional assumption that the dollar value of health does not change over time, a point that is not always recognized in standard arguments. Copyright © 2011 John Wiley & Sons, Ltd.
The histone deacetylase 4/SP1/microrna-200a regulatory network contributes to aberrant histone acetylation in hepatocellular carcinoma
Authors: Yuan Ji-hang , Yang Fu , Chen Bi-feng , Lu Zhi , Huo Xi-song , Zhou Wei-ping , Wang Fang , Sun Shu-han 
Published: August 2011

Abstract

As an important epigenetic mechanism, histone acetylation modulates the transcription of many genes and plays important roles in hepatocellular carcinoma (HCC). Aberrations in histone acetylation have been observed in HCC, but the factors that contribute to the aberrations have not been fully elucidated. MicroRNAs (miRNAs), which are noncoding RNAs that regulate gene expression, are involved in important epigenetic mechanisms. In this study, we determined that miR-200a and the level of histone H3 acetylation at its promoter were reduced in human HCC tissues in comparison with adjacent noncancerous hepatic tissues. Furthermore, our results suggested that the histone deacetylase 4 (HDAC4) inhibited the expression of miR-200a and its promoter activity and reduced the histone H3 acetylation level at the

mir-200a

promoter through a Sp1-dependent pathway. Interestingly, we observed that the miR-200a directly targeted the 3′-untranslated region of the HDAC4 messenger RNA and repressed expression of HDAC4. Therefore, miR-200a ultimately induced its own transcription and increased the histone H3 acetylation level at its own promoter. Through targeting HDAC4, miR-200a also induced the up-regulation of total acetyl-histone H3 levels and increased the histone H3 acetylation level at the

p21

WAF/Cip1

promoter. Finally, we determined that miR-200a inhibited the proliferation and migration of HCC cells

in vivo

and

in vitro

.

Conclusion:

Our findings suggest that the HDAC4/Sp1/miR-200a regulatory network induces the down-regulation of miR-200a and the up-regulation of HDAC4 in HCC. As a result, down-regulation of miR-200a enhances the proliferation and migration of HCC cells and induces aberrant histone acetylation in HCC. These findings highlight a potential therapeutic approach in targeting the HDAC4/Sp1/miR-200a regulatory network for the treatment of HCC. (Hepatology 2011
Methionine adenosyltransferase 1A gene deletion disrupts hepatic very low-density lipoprotein assembly in mice
Authors: Cano Ainara , Buqué Xabier , Martínez-Uña Maite , Aurrekoetxea Igor , Menor Ariane , García-Rodríguez Juan L. , Lu Shelly C. , Martínez-Chantar Luz M. , Mato José M. , Ochoa Begoña , Aspichueta Patricia 
Published: August 2011

Abstract

Very low-density lipoprotein (VLDL) secretion provides a mechanism to export triglycerides (TG) from the liver to peripheral tissues, maintaining lipid homeostasis. In nonalcoholic fatty liver disease (NAFLD), VLDL secretion disturbances are unclear. Methionine adenosyltransferase (MAT) is responsible for

S

-adenosylmethionine (SAMe) synthesis and MAT I and III are the products of the

MAT1A

gene. Deficient MAT I and III activities and SAMe content in the liver have been associated with NAFLD, but whether

MAT1A

is required for normal VLDL assembly remains unknown. We investigated the role of

MAT1A

on VLDL assembly in two metabolic contexts: in 3-month-old

MAT1A

-knockout mice (3-KO), with no signs of liver injury, and in 8-month-old

MAT1A

-knockout mice (8-KO), harboring nonalcoholic steatohepatitis. In 3-KO mouse liver, there is a potent effect of

MAT1A

deletion on lipid handling, decreasing mobilization of TG stores, TG secretion in VLDL and phosphatidylcholine synthesis via phosphatidylethanolamine

N

-methyltransferase.

MAT1A

deletion also increased VLDL– apolipoprotein B secretion, leading to small, lipid-poor VLDL particles. Administration of SAMe to 3-KO mice for 7 days recovered crucial altered processes in VLDL assembly and features of the secreted lipoproteins. The unfolded protein response was activated in 8-KO mouse liver, in which TG accumulated and the phosphatidylcholine-to-phosphatidylethanolamine ratio was reduced in the endoplasmic reticulum, whereas secretion of TG and apolipoprotein B in VLDL was increased and the VLDL physical characteristics resembled that in 3-KO mice.

MAT1A

deletion also altered plasma lipid homeostasis, with an increase in lipid transport in low-density lipoprotein subclasses and decrease in high-density lipoprotein subclasses.

Conclusion:

MAT1A

is required for normal VLDL assembly and plasma lipid homeostasis in mice. Impaired VLDL synthesis, mainly due to SAMe deficiency, contributes to NAFLD development in

MAT1A

-KO mice. (Hepatology 2011