bluuebunny/crossref_metadata_2025_split huggingface dataset:
Arginine Methylation-dependent TRIM47 Stability Mediated by CARM1 Promotes the Metastasis of Hepatocellular Carcinoma
Authors: Hu Jia , Tang Yuzhe , Meng Xiang , Luo Xia , Yao Wen Tao , Tian Li , Zhang Zijian , Zhao Yuan , Zhu Haichuan , Xiao Juan 
Published: June 2024

Abstract

The tripartite motif (TRIM) protein family has been shown to play important roles in the occurrence and development of various tumors. However, the biological functions of TRIM47 and its regulatory mechanism in hepatocellular carcinoma (HCC) remain unexplored. Here, we showed that TRIM47 was upregulated in HCC tissues compared with adjacent normal tissues, especially at advanced stages, and associated with poor prognosis in HCC patients. Functional studies demonstrated that TRIM47 enhanced the migration and invasion ability of HCC cells in vitro and in vivo. Mechanistically, TRIM47 promotes HCC metastasis through interacting with SNAI1 and inhibiting its degradation by proteasome. Moreover, TRIM47 was di-methylated by CARM1 at its arginine 210 (R210) and arginine 582 (R582), which protected TRIM47 from the ubiquitination and degradation mediated by E3 ubiquitin ligase complex CRL4CRBN. Collectively, our study reveals a pro-metastasis role of TRIM47 in HCC, unveils a unique mechanism controlling TRIM47 stability by CARM1 mediated arginine methylation, and highlights the role of the CARM1-CRL4CRBN-TRIM47-SNAI1 axis in HCC metastasis. This work may provide potential therapeutic targets for metastatic HCC treatment.
The effect of yttrium oxide nanoparticles on memory, inflammatory responses and mitochondrial biogenesis in cholestatic male Wistar rats
Authors: Khaledi Sara , Amiri Zeynab , Esfahani Delaram Eslimi , Dargahi Leila , Beirami Elmira 
Published: June 2024

Abstract

Background and Objectives: Cholestasis can lead to oxidative stress, inflammation, apoptosis, mitochondrial dysfunction and ultimately causes cognitive damage, such as memory malfunctions. Considering their anti-inflammatory and protective effects, nanoparticles may be effective for the treatment of neurological disorders or for transferring medications through the blood-brain barrier. This study investigated the protective effect of yttrium oxide nanoparticles (Y2O3NPs) on cognitive disorders, inflammatory response and mitochondrial biogenesis caused by cholestasis in rat hippocampus.
Methods: Male Wistar rats were randomly divided into seven groups: control, sham, vehicle, cholestasis, and three groups of cholestatic rats, which received doses of 0.1, 0.3, and 0.5 mg/kg Y2O3NPs, respectively for 21 days. The Morris water maze, passive avoidance, and elevated plus maze tests were used to assess the learning and memory of the rats. The expression of genes involved in mitochondrial biogenesis (PGC-1α, NRF-1, and TFAM) and pro-inflammatory genes (TNF-α, IL-6, and IL-1β) were evaluated by real-time PCR technique.
Results: Cholestasis led to learning and memory dysfunctions, decreased the expression of genes involved in mitochondrial biogenesis, and increased the expression of genes involved in neuroinflammation. Intraperitoneal injection (IP) of Y2O3NPs, especially at a dose of 0.5 mg/kg, enhanced the recognition and recall memory, increased the expression of factors involved in mitochondrial biogenesis (PGC-1α, NRF-1, and TFAM), and decreased neuroinflammation (TNF-α, IL-6, and IL-1β).
Conclusion: This study demonstrated that Y2O3NPs reduced memory disorders caused by cholestasis. This nanoparticle increased the expression of factors involved in mitochondrial biogenesis, reduced the inflammatory responses in the hippocampus of cholestasis animals, and possibly alleviated cognitive disorders through this mechanism.
The Link between Osteoporosis and Frozen Shoulder: Exploring the Therapeutic Effect of TAK715 on Reversing Fibrosis and Protecting against Osteoporosis via the p38 MAPK Signaling Pathway
Authors: Li Xinhao , Yan Yan , Long Yi , Hou Jingyi , Wang Zhuo , Meng Yuhan , Cui Dedong , Li Ming , Yang Rui 
Published: June 2024

Abstract

Background:
The global incidence of frozen shoulder (FS) and osteoporosis (OP) is high. Although FS patients may experience localized OP in the shoulder, there is still insufficient strong evidence to confirm the relationship between FS and OP. The main objective of this current research is to investigate the pathogenesis mechanism of FS and explore the connection between FS and OP.
Methods:
We obtained FS and OP datasets from GEO and identified crosstalk genes. We screened the p38 MAPK signaling pathway and its specific inhibitor, TAK715. We conducted flow cytometry, western blot, and PCR analyses to assess the treatment effect of TAK715 on FS synovium fibroblasts at concentrations of 1μM, 5μM, and 10μM. Additionally, we employed SD rats to validate the treatment effects of TAK715 in vivo.
Results:
TAK715 was found to also intervene in the activation of osteoclasts. We successfully established a FS and OP rat model, with the FS with OP rat displaying more severe symptoms. Results demonstrated that TAK715 effectively corrected both fibrosis and osteoporosis simultaneously in vivo.
Conclusions:
The frozen shoulder with osteoporosis may exhibit more severe symptoms, and TAK715 is effective in protecting fibrosis and osteoporosis both in vitro and vivo. The therapy to correct FS and OP simultaneously by TAK715 provides novel approach in FS treatment and study.
Inhibit of the cGAS-STING-STAT1 pathway protects heart from the Doxorubicin-induced cardiotoxicity
Authors: Hou Ning , YUAN Xun , Tian Wenqi , Qin Yuan , Jiang Ruchao , Lu Xianneng , Zhu Qiulian , Zhang Guiping , Liu Yun , Jiang Wenyue 
Published: June 2024

Abstract

Doxorubicin (DOX) is a common clinical chemotherapeutic drug. However, DOX-induced cardiotoxicity (DIC) limits the wide and long-term clinical use to treat cancers. This study aims to dissect the mechanism in which DNA damage-triggered micronucleus (MN) formation activates the cyclic GMP-AMP synthase (cGAS)-stimulator of interferon genes (STING)-STAT1 pathway in cardiac fibroblasts during DIC. C57BL/6J mice were intravenously injected with 10 mg/kg of DOX to establish an acute DOX-induced cardiac injury mouse model. Meanwhile, C57BL/6J mice were intraperitoneally injected with STING inhibitor C-176 (10 mg/kg/week) or intravenously injected with STING siRNA (10 nM/week) prior to DOX (5 mg/kg/week) intravenous injection for 4 weeks to establish a chronic DIC mouse model. After 1 week of Dox injection, mice were harvested for further analysis. Measurements included echocardiography, immunohistochemical analyses, Masson and Sirius Red staining, and Western blots. Here, we showed that the cGAS-STING-STAT1 pathway was activated in cardiac fibroblasts during DIC. The STING inhibition by C-176 or the STING knockdown via siRNA in DOX-induced chronic cardiotoxicity mouse heart attenuated the DOX-induced cardiac dysfunction, cardiac fibrosis, and the inflammatory response. Mechanistically, we also demonstrated that the DOX-induced DNA damage-triggered MN formation impaired the nuclear stability, initiating the activation of the cGAS-STING-STAT1 pathway in cardiac fibroblasts during DIC. Our study illustrated that the activation of the cGAS-STING-STAT1 pathway initiated by DOX-induced DNA damage and MN formation stimulated proinflammatory responses in cardiac fibroblasts, thus promoting myocardial fibrosis during DIC.
Comparing the effectiveness of radial Extracorporeal Shockwave therapy (rESWT) and three conventional treatment approaches for biceps brachii muscle in exercise-induced Delayed Onset of Muscle Soreness (DOMS): A Protocol for a Randomized Clinical Trial
Authors: Islam Azharul , Hossain K. M. Amran , Kabir Md. Feroz , Jahan Sharmila , Rahman Ehsanur , Hossain Md. Kabir , Hossain Md. Zahid 
Published: June 2024

Abstract

Background
Delayed onset of muscle soreness (DOMS) is a typical physiological response to unaccustomed to intense exercise, characterised by pain, stiffness, and reduced muscle function. DOMS is often experienced in muscles after strenuous limb activities, impacting an individual's physical performance and overall well-being. Effective management strategies for DOMS are essential to minimise discomfort and enhance recovery. In the treatment of DOMS, physiotherapy management is a better option rather than analgesics or NSAIDs. There is a research gap on radial extracorporeal shockwave therapy, rESWT, MFR, cryotherapy, etc. that has an individual and unique effect on pain reduction and functional improvement.
Method
An assessor and participants-blinded randomised clinical trial (RCT) will be used for the investigation. The impact of rESWT, MFR, and stretching combined with cryotherapy on exercise-induced DOMS will be examined in this study. On the patient, DOMS will be induced for research purposes. Three groups will receive rESWT, MFR, and stretching in addition to cryotherapy. After DOMS is induced, the treatment will be given once daily for 0 to 48 hours (3 times in 3 days). Each group will include 40 students, and the research will measure how rESWT, MFR, Cryotherapy, and Stretching affect pain alleviation before treatment (pretest), immediately after treatment (post-test 1), 24 hours later (post-test 2), 48 hours later (post-test 3) and 72 hours later (post-test 4). The outcome metric will be pain intensity and ROM. Male and female students of the University with good physical health and without a history of taking painkillers recently make up the study’s groups.
Discussion
The study investigates the efficacy of physiotherapy approaches (rESWT, MFR, Cryotherapy, and Stretching) on exercise-induced DOMS. It addresses a research gap regarding rESWT’s effectiveness and offers valuable insights into pain reduction methods. The findings can guide comprehensive pain management and individualised recovery strategies.
Trial registration
CTRI/2023/08/056649 [Registered on: 18/08/2023]
Food web structure and species’ role in an oceanic Marine Protected Area in the subantarctic
Authors: Scian Melina , Riccialdelli Luciana , Marina Tomás Ignacio 
Published: June 2024

Abstract

The Marine Protected Area Yaganes (MPAY) was created in response to the great deterioration that has been suffering the ecosystems due to the environmental crisis that is affecting our planet, with the aim to protect key ocean habitats and species. Located at the southern tip of South America, MPAY is home to a wide variety of marine biodiversity and habitats, which makes it of ​​special interest for the conservation of subantarctic environments. For the first time, we described the potential trophic interactions within the MPAY, with emphasis in the pelagic realm. We applied both a network-level and a species-level approach to characterise the structure and complexity properties, aiming to comprehend trophic dynamics and the specific roles of certain species within the food web. In terms of complexity, the MPAY food web consists of 127 species with 658 predator-prey interactions, a density of interactions of 5.18 and a connectance of 0.04. Regarding its structure, around 69% of the species occupied intermediate trophic levels. The Keystone Species Index (KSI), highlighted the importance of some species such as the Patagonian toothfish (Dissostichus eleginoides), the amphipod Themisto gaudichaudii, euphausiids and myctophids, among others, to the structure of the food web.
Characterization of shared neoantigens in Endometrial Cancer with Microsatellite Instability
Authors: Urbani Andrea , De Paolis Elisa , Nero Camilla , Micarelli Elisa , Leoni Guido , Piermattei Alessia , Trozzi Rita , Scarselli Elisa , D'Alise Anna Morena , Giacò Luciano , De Bonis Maria , Daniele Gennaro , Piana Diletta , Pasciuto Tina , Zannoni Gian Franco , Minucci Angelo , Scambia Giovanni , Fanfani Francesco 
Published: June 2024

Abstract

Endometrial cancer (EC) with microsatellite instability (MSI) are characterized by the accumulation of insertions/deletions at microsatellite sites, mainly mononucleotide repeats (MNRs). These MSI-related mutations lead to the synthesis of frameshift peptides (FSPs) that represent tumor-specific neoantigens (nAg) proved to be shared across patients/tumors with MSI. In this study we explored the feasibility of a nAg-based cancer vaccination design in EC with MSI. We adopted a whole exome sequencing approach and ad hoc bioinformatics pipelines to characterize FSPs in 35 patients with EC. A mean of 100 mutated MNRs was identified with an enrichment in patients’ group with MLH1 impairment. A high coverage emerged from the comparative analysis of the EC FSPs with the content of the previously validated NOUS-209 vaccine. We also obtained evidences of translation of EC FSPs as expressed proteins from Riboseq data, supporting the potential as target of vaccination. The development of a nAgs-based vaccine strategy in EC with MSI may be further explore. Cancer vaccination in combination with checkpoint inhibition could represent an enhancement of the immunotherapeutic approach in EC.
Applicability of Examining the Wavelet Packet Decomposition on Statistical Models for River Stage Forecasting
Authors: Bashir Adnan , Shehzad Ahmed 
Published: June 2024

Abstract

River streamflow forecasting is important for managing and controlling the water resource system. This study developed new hybrid models, namely wavelet packet first-order response surface (WPFORS) and wavelet packet quadratic response surface (WPQRS), using the wavelet packet decomposition technique with the first-order response surface (FORS) and quadratic response surface (QRS) models. This study is also based on forecasting the performance of the three traditional models: multiple linear regression (MLR), FORS, and QRS. The wavelet packet decomposition technique is used to remove noise from hydrological data. The daily streamflow data from the 2005 to 2013 monsoon season (1st July to 31st September) of the Chenab River basin in Pakistan were used. To check the forecasting performance of the observed models, the criteria used are root mean square error (RMSE), mean square error (MSE), Nash Sutcliffe coefficient of efficiency (NSE), and mean absolute error (MAE). The results found that WPFORS and WPQRS presented better forecasting accuracy than traditional models like MLR, QRS, and FORS. In addition, it is also observed that the overall performance of WPQRS is better than the WPFORS model for 1-d ahead forecasting of streamflow data.
Preferences for individual types of physical activity among youth: trend and challenges in the context of the pandemic
Authors: Kudlacek Michal , Fromel Karel , Groffik Dorota , Mitas Josef 
Published: June 2024

Abstract

Background
The global decline in physical activity among the youth has been exacerbated by the pandemic's impact on their lifestyles. Addressing these adverse effects post-pandemic by supporting preferred physical activities among young individuals is crucial. The aim of the study is to identify the status and trend of preferences for individually focused PA of young people in the context of the negative effects of the pandemic on the physical activity of young people.
Methods
Conducted between 2007 and 2020, this research encompassed 19,235 participants aged 15 to 26. Physical activity preferences were gauged using the Questionnaire on Physical Activity Preferences, while actual physical activity levels were measured through the International Physical Activity Questionnaire-Long Form.
Results
Among the participants, individual physical activities were favored by 24% of Czech and 25% of Polish boys, and 23% of Czech and 25% of Polish girls. Across all demographics, swimming and cycling emerged as preferred activities, with a notable increase in running. A positive correlation was found between the preference for running and higher levels of recreational, vigorous, and overall weekly physical activity. Additionally, an increased preference for running was associated with a higher likelihood of meeting weekly physical activity recommendations for both girls (OR = 1.82, CI = 1.62–2.04, p < 0.001) and boys (OR = 1.61, CI = 1.44–1.82, p < 0.001) in both countries.
Conclusions
The systematic and long-term monitoring of physical activity preferences is vital for effectively promoting and supporting a healthy lifestyle among youth, especially during periods of social and health crises.
Comprehensive Investigation of Ergonomic Challenges and Work-related Musculoskeletal Disorders among Intensive Care Unit Healthcare Providers of Western India through Mixed Methods Study
Authors: Detroja Sonali , Mahajan Rajkumar , Sheth Ankit 
Published: June 2024

Abstract

Background: Work-related musculoskeletal disorders (WRMSDs) are significant concern in healthcare sector, specifically intensive care units (ICUs) due to distinct work environment. This study aims to comprehensively investigate determinants of WRMSDs and ergonomic challenges, specific to ICU healthcare providers, providing valuable insights for targeted interventions.
Methods: In this mixed methods study, 200 consenting healthcare providers from seven ICUs participated. Structured questionnaires were used to collect details of socio-demographics, occupation, physical and workplace factors, risk perception, and musculoskeletal pain through modified Nordic Musculoskeletal Questinnaire. Qualitative insights were obtained through in-depth interviews until saturation of responses. Statistical analyses included inferential statistics, logistic regression, and thematic analysis.
Results: Study revealed a high prevalence of WRMSDs (84%), with low back pain and neck reported to be most affected regions. Logistic regression analysis identified longer tenure in the ICU, high shift frequency, high physical workload and risk perception, and low exercise habits as significant predictors of WRMSDs among ICU healthcare providers. Qualitative study underscored challenges of heavy patient loads, long hours, and emotional strain faced by ICU healthcare providers, while also highlighting institutional support strategies for prevention of WRMSDs.
Conclusion: This study offers valuable insights into the multifaceted challenges faced by ICU healthcare providers and underscores the importance of multidimensional interventions incorporating physical, behavioural, and environmental factors. There is urgent need to address modifiable risk factors, including ergonomic training, workplace modifications, schedule adjustments, and educational programs to enhance risk awareness and preventive behaviours to effectively combat the burden of WRMSDs.
The diuretic effect of Goreisan involves the modulation of aquaporin 2 localization via the calcium-sensing receptor
Authors: Ogura Keisuke , Fujitsuka Naoki , Nahata Miwa , Tokita Yohei 
Published: June 2024

Abstract

Aquaporin 2 (AQP2) contributes to water reabsorption and primitive urine concentration by migrating to the luminal surface of the collecting ducts in an anti-diuretic hormone-stimulated manner, and the signaling pathway involved in AQP2 localization is a target for diuretics. This study investigated whether AQP2 is involved in the diuretic effect and mechanisms of Goreisan (GRS), a traditional Japanese Kampo medicine used to treat symptoms such as edema in patients with decreased urination. GRS exerted diuretic effects on desmopressin (dDAVP)-induced decreases in urine output and inhibited phosphorylation of AQP2 at Ser269 in the renal tissues of mice. Furthermore, GRS inhibited the migration of phosphorylated AQP2 to the luminal side following forskolin (FSK) stimulation using a 3D culture model of the kidney collecting duct cell line mIMCD-3. GRS induced a transient increase in the intracellular calcium concentration via the calcium-sensing receptor (CaSR) and suppressed the FSK-stimulated increase in cAMP production. These results suggest that GRS regulates urine volume by modulating the localization of AQP2 via CaSR.
Energy Efficient Optimized Clustering for Free Space Optical Communication in Software Defined Underwater WSN
Authors: Chauhan Kanika , Sharma Deepak Kumar , Sharma Nonita , Tushir Bhagyashri 
Published: June 2024

Abstract

The utilization of Free-Space Optical (FSO) communication in underwater environments is gaining momentum, particularly in Software Defined Underwater Wireless Sensor Networks(SDUWSNs). However, challenges such as high-energy loss and limited propagation distance persist in data transmission within SDUWSNs. In addressing these issues, this study introduces an innovative approach known as Software Defined Free Space Optical Underwater Wireless Sensor Networks, where FSO communication is seamlessly integrated with SDUWSNs to enhance network longevity. To optimize the performance of SD-FSO-UWSNs, the implementation of clustering and routing is explored as an effective strategy for energy-efficient data delivery. Nevertheless, the selection of optimal control nodes (CNs) in clustering poses a significant challenge. In response, a novel self-adaptive cheetah optimization-based clustering approach (SACO-CA) is proposed by incorporating self-adaptive inertia weights to identify optimal CNs based on a devised fitness value. The fitness function considers important parameters such as energy levels and distances among network devices, aiming to balance cluster sizes effectively. Moreover, the NS3 simulator is used to run network simulation while, SDN policies are implemented through the Open Network Oper-ating System (ONOS) controller. The simulation result metrics, including stability period,alive nodes, average residual energy, packets transmitted to the control server, and averagedelay, indicate that SACO-CA outperforms existing state-of-the-art methods. The results underscore the efficacy of the nature-inspired SACO-CA approach in optimizing CNs and improving overall network performance in SD-FSO-UWSNs.