bluuebunny/crossref_metadata_2025_split huggingface dataset:
Summable formal invariant curves of diffeomorphisms
Authors: LÓPEZ-HERNANZ L. 
Published: March 2011

AbstractLet F be a tangent to the identity diffeomorphism in (ℂ2,0) and X its infinitesimal generator. We prove that Camacho and Sad’s formal invariant curves of X give summable formal power series, whose sums correspond to the parabolic curves found by Hakim for F and F−1.
Architectural space as a moulding factor of care practices and resident privacy in assisted living
Authors: NORD CATHARINA 
Published: March 2011

ABSTRACTThis article presents an analysis of privacy, care practices and architectural space in assisted living in Sweden. The presented research is a qualitative case study. Observations and personal interviews with staff as well as residents were the major data collection methods. The analysis revealed the evasiveness of a private–public dichotomy; that is, how privacy appears in public spaces and how private spaces became public under certain conditions. During the course of a day, the residents' privacy was qualified and structured by caring activities that took place in various spaces and that associated with variable distance or closeness to the staff. The study shows that individualised care practices improved privacy for the resident, and that although architectural features constrained the staff, they used a number of spatial strategies to promote the residents' privacy, for instance, in the dining room at meal times or when residents were subject to intimate care in their private rooms. Access and control are dimensions of privacy that are relevant to assisted living. The residents had more control of access to their private rooms than control of their personal space in public areas. Individualised care strengthened the residents' agency. Staff supported the residents to lead a private life in the assisted-living facility.
Environmental issues of groundwater in Korea: implications for sustainable use
Authors: LEE J.Y. 
Published: March 2011

SUMMARYGroundwater has been extensively exploited worldwide but is now confronted by a variety of problems, including groundwater depletion and contamination, that threaten its sustainable use as a clean water source. Groundwater is one of the major sources of water for domestic, agricultural and industrial uses, and provides 13% of the total annual water supply in Korea. Annual groundwater use has continuously increased from 2.57 billion m3 in 1994 to 3.72 billion m3 in 2007, of which 48.1% was consumed for domestic purposes. However, due to imprudent groundwater development and inappropriate management, Korea has confronted some critical groundwater problems, including extensive water level decline and quality deterioration caused by petroleum hydrocarbons and chlorinated solvents. Among 193 national groundwater deep-monitoring wells nationwide, 62% showed decreasing water levels over the period 2004–2008. Soil and groundwater contamination by petroleum hydrocarbons was detected at a great number of military bases and public facilities, which drew national attention and complaints. The presence of high levels of radionuclides such as uranium and radon in groundwater has awakened controversy on their health effects. Increasing outbreaks of massive gastroenteritis were attributed to noroviruses in contaminated groundwater, and raised public health concerns. In addition, chlorinated solvents, especially trichloroethylene (TCE), have been frequently found in urban and industrial groundwaters, further adding to the burdens of environmental authorities. Consequently, these groundwater-related environmental issues have forced the Korean government and relevant authorities to urgently devise mitigation plans to secure a sustainable future use of groundwater resources. This paper provides details of the groundwater issues and implications for appropriate development and management.
Spread of West Nile virus in Iran: a cross-sectional serosurvey in equines, 2008–2009
Authors: AHMADNEJAD F. , OTAROD V. , FALLAH M. H. , LOWENSKI S. , SEDIGHI-MOGHADDAM R. , ZAVAREH A. , DURAND B. , LECOLLINET S. , SABATIER P. 
Published: March 2011

SUMMARYWe report the first large-scale serosurvey for West Nile virus (WNV) conducted in the equine population in Iran. Blood samples were obtained in 2008–2009 from 1054 equines collected from 260 districts located in 27 provinces. The overall seroprevalence rate for WNV neutralizing antibodies was 23·7%. Marked geographical variations were observed as province-specific seroprevalence rates ranged from 1% to 88%, the highest values being observed in the southern and western parts of the country. The presence of IgM-positive animals (n=9) indicated a recent circulation of WNV in several provinces. Logistic modelling confirmed this result with a significant effect of age on seropositivity. This study revealed extensive circulation of WNV in Iran particularly in southwestern provinces where the virus probably circulates every year.
Molecular characterization of group A rotaviruses detected in children with gastroenteritis in Ireland in 2006–2009
Authors: CASHMAN O. , COLLINS P. J. , LENNON G. , CRYAN B. , MARTELLA V. , FANNING S. , STAINES A. , O'SHEA H. 
Published: March 2011

SUMMARYCommunity and hospital-acquired cases of human rotavirus are responsible for millions of gastroenteritis cases in children worldwide, chiefly in developing countries, and vaccines are now available. During surveillance activity for human rotavirus infections in Ireland, between 2006 and 2009, a total of 420 rotavirus strains were collected and analysed. Upon either PCR genotyping and sequence analysis, a variety of VP7 (G1–G4 and G9) and VP4 (P[4], P[6], P[8] and P[9]) genotypes were detected. Strains G1P[8] were found to be predominant throughout the period 2006–2008, with slight fluctuations seen in the very limited samples available in 2008–2009. Upon either PCR genotyping and sequence analysis of selected strains, the G1, G3 and G9 viruses were found to contain E1 (Wa-like) NSP4 and I1 VP6 genotypes, while the analysed G2 strains possessed E2 NSP4 and I2 VP6 genotypes, a genetic make-up which is highly conserved in the major human rotavirus genogroups Wa- and Kun-like, respectively. Upon sequence analysis of the most common VP4 genotype, P[8], at least two distinct lineages were identified, both unrelated to P[8] Irish rotaviruses circulating in previous years, and more closely related to recent European humans rotaviruses. Moreover, sequence analysis of the VP7 of G1 rotaviruses revealed the onset of a G1 variant, previously unseen in the Irish population.
Epidemiological and molecular assessment of a measles outbreak in a highly vaccinated population of northeast Italy
Authors: D'AGARO P. , MOLIN G. DAL , GALLO T. , ROSSI T. , SANTON D. , BUSETTI M. , COMAR M. , CAMPELLO C. 
Published: March 2011

SUMMARYTwo distinct measles outbreaks, unrelated from the epidemiological point of view but caused by genetically related strains, occurred in the Friuli Venezia Giulia region of northeastern Italy. Forty-two cases were reported during the period April–May 2008. In the first outbreak the index case was a teacher who introduced the virus into the Pordenone area, involving eight adolescents and young adults. The other concomitant outbreak occurred in the city of Trieste with 33 cases. The containment of the epidemics can be explained by the high MMR vaccine coverage in an area where the first dose was delivered to 93·4% and the second dose to 88·3% of the target children. Phylogenetic analysis of 14 measles virus strains showed that they belonged to a unique D4 genotype indistinguishable from the MVs/Enfield.GBR/14.07 strain, probably introduced from areas (i.e. Piedmont and Germany) where this genotype was present or had recently caused a large epidemic.
Modelling immunization strategies with cytomegalovirus vaccine candidates
Authors: AZEVEDO R. S. , AMAKU M. 
Published: March 2011

SUMMARYIn order to analyse the impact of vaccination against cytomegalovirus (CMV) on congenital infection incidence using current vaccines tested in phase II clinical trials, we simulated different scenarios by mathematical modelling, departing from the current vaccine characteristics, varying age at vaccination, immunity waning, vaccine efficacy and mixing patterns. Our results indicated that the optimal age for a single vaccination interval is from 2 to 6 months if there is no immunity waning. Congenital infection may increase if vaccine-induced immunity wanes before 20 years. Congenital disease should increase further when the mixing pattern includes transmission among children with a short duration of protection vaccine. Thus, the best vaccination strategy is a combined schedule: before age 1 year plus a second dose at 10–11 years. For CMV vaccines with low efficacy, such as the current ones, universal vaccination against CMV should be considered for infants and teenagers.
Sporadic Legionnaires' disease: the role of domestic electric hot-water tanks
Authors: DUFRESNE S. F. , LOCAS M. C. , DUCHESNE A. , RESTIERI C. , ISMAÏL J. , LEFEBVRE B. , LABBÉ A. C. , DION R. , PLANTE M. , LAVERDIÈRE M. 
Published: March 2011

SUMMARYSporadic community-acquired legionellosis (SCAL) can be acquired through contaminated aerosols from residential potable water. Electricity-dependent hot-water tanks are widely used in the province of Quebec (Canada) and have been shown to be frequently contaminated withLegionellaspp. We prospectively investigated the homes of culture-proven SCAL patients from Quebec in order to establish the proportion of patients whose domestic potable hot-water system was contaminated with the sameLegionellaisolate that caused their pneumonia. Water samples were collected in each patient's home. Environmental and clinical isolates were compared using pulsed-field gel electrophoresis. Thirty-six patients were enrolled into the study.Legionellawas recovered in 12/36 (33%) homes. The residential and clinical isolates were found to be microbiologically related in 5/36 (14%) patients. Contaminated electricity-heated domestic hot-water systems contribute to the acquisition of SCAL. The proportion is similar to previous reports, but may be underestimated.
Field astrobiology research in Moon–Mars analogue environments: instruments and methods
Authors: Foing B.H. , Stoker C. , Zavaleta J. , Ehrenfreund P. , Thiel C. , Sarrazin P. , Blake D. , Page J. , Pletser V. , Hendrikse J. , Direito S. , Kotler J.M. , Martins Z. , Orzechowska G. , Gross C. , Wendt L. , Clarke J. , Borst A.M. , Peters S.T.M. , Wilhelm M.-B. , Davies G.R. ,   ILEWG EuroGeoMars 2009 Team
Published: March 2011

AbstractWe describe the field demonstration of astrobiology instruments and research methods conducted in and from the Mars Desert Research Station (MDRS) in Utah during the EuroGeoMars campaign 2009 coordinated by ILEWG, ESA/ESTEC and NASA Ames, with the contribution of academic partners. We discuss the entire experimental approach from determining the geological context using remote sensing, in situ measurements, sorties with sample collection and characterization, analysis in the field laboratory, to the post sample analysis using advanced laboratory facilities.We present the rationale for terrestrial field campaigns to strengthen astrobiology research and the link between in situ and orbital remote sensing data. These campaigns are supporting the preparation for future missions such as Mars Science Laboratory, ExoMars or Mars Sample Return. We describe the EuroGeoMars 2009 campaign conducted by MDRS crew 76 and 77, focused on the investigation of surface processes in their geological context. Special emphasis was placed on sample collection and pre-screening using in-situ portable instruments. Science investigations included geological and geochemical measurements as well as detection and diagnostic of water, oxidants, organic matter, minerals, volatiles and biota.EuroGeoMars 2009 was an example of a Moon–Mars field research campaign dedicated to the demonstration of astrobiology instruments and a specific methodology of comprehensive measurements from selected sampling sites. We discuss in sequence: the campaign objectives and trade-off based on science, technical or operational constraints. This includes remote sensing data and maps, and geological context; the monitoring of environmental parameters; the geophysical context and mineralogy studies; geology and geomorphology investigations; geochemistry characterization and subsurface studies.We describe sample handling (extraction and collection) methods, and the sample analysis of soils and rocks performed in the MDRS laboratory using close inspection, initial petrological characterization, microscopy, Visible-NIR spectrometry, Raman spectrometry, X-ray diffraction/X-ray fluorescence spectrometry, soil analysis, electrochemical and biological measurements.The results from post-mission analysis of returned samples using advanced facilities in collaborator institutes are described in companion papers in this issue. We present examples of in-situ analysis, and describe an example investigation on the exploration and analysis of endolithic microbial mats (from reconnaissance, in-situ imaging, sampling, local analysis to post-mission sample analysis).
Academic cross-fertilization by public screening yields a remarkable class of protein phosphatase methylesterase-1 inhibitors
Authors: Bachovchin Daniel A. , Mohr Justin T. , Speers Anna E. , Wang Chu , Berlin Jacob M. , Spicer Timothy P. , Fernandez-Vega Virneliz , Chase Peter , Hodder Peter S. , Schürer Stephan C. , Nomura Daniel K. , Rosen Hugh , Fu Gregory C. , Cravatt Benjamin F. 
Published: March 2011

National Institutes of Health (NIH)-sponsored screening centers provide academic researchers with a special opportunity to pursue small-molecule probes for protein targets that are outside the current interest of, or beyond the standard technologies employed by, the pharmaceutical industry. Here, we describe the outcome of an inhibitor screen for one such target, the enzyme protein phosphatase methylesterase-1 (PME-1), which regulates the methylesterification state of protein phosphatase 2A (PP2A) and is implicated in cancer and neurodegeneration. Inhibitors of PME-1 have not yet been described, which we attribute, at least in part, to a dearth of substrate assays compatible with high-throughput screening. We show that PME-1 is assayable by fluorescence polarization-activity-based protein profiling (fluopol-ABPP) and use this platform to screen the 300,000+ member NIH small-molecule library. This screen identified an unusual class of compounds, the aza-β-lactams (ABLs), as potent (IC
50
values of approximately 10 nM), covalent PME-1 inhibitors. Interestingly, ABLs did not derive from a commercial vendor but rather an academic contribution to the public library. We show using competitive-ABPP that ABLs are exquisitely selective for PME-1 in living cells and mice, where enzyme inactivation leads to substantial reductions in demethylated PP2A. In summary, we have combined advanced synthetic and chemoproteomic methods to discover a class of ABL inhibitors that can be used to selectively perturb PME-1 activity in diverse biological systems. More generally, these results illustrate how public screening centers can serve as hubs to create spontaneous collaborative opportunities between synthetic chemistry and chemical biology labs interested in creating first-in-class pharmacological probes for challenging protein targets.
First Record of Phytophthora nicotianae Causing Leaf Blight on Rhododendron in Greece
Authors: Tsopelas P. , Paplomatas E. J. , Tjamos S. E. , Elena K. 
Published: March 2011

Species of Rhododendron and resulting hybrids are very important hosts of the quarantine pathogen Phytophthora ramorum, since they play a major role in the spread of the pathogen in Europe. However, many other Phytophthora species infect these hosts, causing similar symptoms. Widmer (4) listed 17 Phytophthora species as foliar pathogens of rhododendron in the United States. A survey was conducted in Greece in October 2009, in which potted plants of Rhododendron spp. were inspected for symptoms of necrotic lesions on leaves and buds caused by P. ramorum. Symptomatic plants were observed in one of the nurseries inspected in the Triphylia Region in southwestern Peloponnese. Isolations from symptomatic leaves on PARBhy-V8 selective agar medium (1) yielded Phytophthora isolates. Colonies on V8 juice agar appeared white and cottony, with a radial growth of 4.2 to 4.6 mm per day at 28°C with a maximum growth temperature of 36°C. Sporangia were produced abundantly on the medium surface and in water; the sporangia were broadly ovoid and papillate and 35 to 50 × 25 to 35 μm. Chlamydospores, 25 to 40 μm in diameter, were observed in 2-week-old cultures, while no sexual structures were observed. Three of the isolates examined were identified as P. nicotianae B. de Haan on the basis of morphological and physiological features (3,4). Genomic DNA was extracted from pure cultures of an isolate and the internal transcribed spacer (ITS) region was amplified using the ITS4/5 primer pair. Sequence analyses by BLAST indicated that the isolates were most similar to P. nicotianae (GenBank Accession No. AJ 854295.1) with sequence identity values of 99%. One of the isolates was deposited in the culture collection of the University of Athens (ATHUM 6519). Detached wounded leaves of Rhododendron hybrid cv. Red Jack were inoculated with agar plugs. Necrotic lesions, similar to those observed in the nursery, appeared on the inoculated leaves after 7 days of incubation at 26°C, while no symptoms developed on control leaves inoculated with sterile agar plugs. The pathogen was consistently reisolated from infected leaves, but not from the controls. P. nicotianae, being a thermophilic species, is the most common Phytophthora species in Greece, reported on more than 30 plant species (2). This pathogen has been reported on Rhododendron spp. in the United States (3,4), but to our knowledge, there was no record of this pathogen on these hosts as yet in Europe and this is the first published report of the pathogen on Rhododendron in Greece. References: (1) A. Belisario et al. Plant Dis. 87:101, 2003. (2) K. Elena. Technical Bulletin No 13. Benaki Phytopathological Institute. Athens, Greece (in Greek), 1999. (3) D. C. Erwin and O. K. Ribeiro. Phytophthora Diseases Worldwide. The American Phytopathological Society, St. Paul, MN, 1996. (4) T. L. Widmer. Online publication. doi: 10.1094/PHP-2010-0317-01-RS, Plant Health Progress, 2010.
Arabidopsis NDR1 Is an Integrin-Like Protein with a Role in Fluid Loss and Plasma Membrane-Cell Wall Adhesion
Authors: Knepper Caleb , Savory Elizabeth A. , Day Brad 
Published: March 2011

Abstract
Arabidopsis (Arabidopsis thaliana) NON-RACE-SPECIFIC DISEASE RESISTANCE1 (NDR1), a plasma membrane-localized protein, plays an essential role in resistance mediated by the coiled-coil-nucleotide-binding site-leucine-rich repeat class of resistance (R) proteins, which includes RESISTANCE TO PSEUDOMONAS SYRINGAE2 (RPS2), RESISTANCE TO PSEUDOMONAS SYRINGAE PV MACULICOLA1, and RPS5. Infection with Pseudomonas syringae pv tomato DC3000 expressing the bacterial effector proteins AvrRpt2, AvrB, and AvrPphB activates resistance by the aforementioned R proteins. Whereas the genetic requirement for NDR1 in plant disease resistance signaling has been detailed, our study focuses on determining a global, physiological role for NDR1. Through the use of homology modeling and structure threading, NDR1 was predicted to have a high degree of structural similarity to Arabidopsis LATE EMBRYOGENESIS ABUNDANT14, a protein implicated in abiotic stress responses. Specific protein motifs also point to a degree of homology with mammalian integrins, well-characterized proteins involved in adhesion and signaling. This structural homology led us to examine a physiological role for NDR1 in preventing fluid loss and maintaining cell integrity through plasma membrane-cell wall adhesions. Our results show a substantial alteration in induced (i.e. pathogen-inoculated) electrolyte leakage and a compromised pathogen-associated molecular pattern-triggered immune response in ndr1-1 mutant plants. As an extension of these analyses, using a combination of genetic and cell biology-based approaches, we have identified a role for NDR1 in mediating plasma membrane-cell wall adhesions. Taken together, our data point to a broad role for NDR1 both in mediating primary cellular functions in Arabidopsis through maintaining the integrity of the cell wall-plasma membrane connection and as a key signaling component of these responses during pathogen infection.
Tomato RAV Transcription Factor Is a Pivotal Modulator Involved in the AP2/EREBP-Mediated Defense Pathway
Authors: Li Chia-Wen , Su Ruey-Chih , Cheng Chiu-Ping , Sanjaya  , You Su-Juan , Hsieh Tsai-Hung , Chao To-Chun , Chan Ming-Tsair 
Published: March 2011

Abstract
Ralstonia solanacearum is the causal agent of bacterial wilt (BW), one of the most important bacterial diseases worldwide. We used cDNA microarray to survey the gene expression profile in transgenic tomato (Solanum lycopersicum) overexpressing Arabidopsis (Arabidopsis thaliana) CBF1 (AtCBF1), which confers tolerance to BW. The disease-resistant phenotype is correlated with constitutive expression of the Related-to-ABI3/VP1 (RAV) transcription factor, ethylene-responsive factor (ERF) family genes, and several pathogenesis-related (PR) genes. Using a transient assay system, we show that tomato RAV2 (SlRAV2) can transactivate the reporter gene driven by the SlERF5 promoter. Virus-induced gene silencing of SlERF5 and SlRAV2 in AtCBF1 transgenic and BW-resistant cultivar Hawaii 7996 plants gave rise to plants with enhanced susceptibility to BW. Constitutive overexpression of SlRAV2 in transgenic tomato plants induced the expression of SlERF5 and PR5 genes and increased BW tolerance, while knockdown of expression of SlRAV2 inhibited SlERF5 and PR5 gene expression under pathogen infection and significantly decreased BW tolerance. In addition, transgenic tomato overexpressing SlERF5 also accumulated higher levels of PR5 transcripts and displayed better tolerance to pathogen than wild-type plants. From these results, we conclude that SlERFs may act as intermediate transcription factors between AtCBF1 and PR genes via SlRAV in tomato, which results in enhanced tolerance to BW.