bluuebunny/crossref_metadata_2025_split huggingface dataset:
Role of iron and sodium citrate in animal protein‐free CHO cell culture medium on cell growth and monoclonal antibody production
Authors: Bai Yunling , Wu Changjian , Zhao Jia , Liu Yan‐Hui , Ding Wei , Ling Wai Lam W. 
Published: October 2010

AbstractChemically defined iron compounds were investigated for the development of animal protein‐free cell culture media to support growth of CHO cells and production of monoclonal antibodies (mAb). Using a multivessel approach of 96‐well plates, shake flasks, and bioreactors, we identified iron and its chemical partner citrate as critical components for maintenance of continuous cell growth and mAb production. The optimized iron concentration range was determined to be 0.1–0.5 mM and that for citrate 0.125–1 mM. This complete formulation is able to maintain cell growth to similar levels as those supplemented with iron compounds alone; however, mAb productivity was enhanced by 30–40% when citrate was present. The addition of sodium citrate (SC) did not affect product quality as determined by size exclusion chromatography, ion exchange chromatography, reversed phase and normal phase‐HPLC. No significant changes in glucose and lactate profiles, amino acid utilization, or mAb heavy and light chain expression ratios were observed. Cellular ATP level was ∼30% higher when SC was included suggesting that SC may have a role in enhancing cellular energy content. When cell lysates were analyzed by LC‐MS to assess the overall cellular protein profile, we identified that in the SC‐containing sample, proteins involved in ribosome formation and protein folding were upregulated, and those functions in protein degradation were downregulated. Taken together, this data demonstrated that iron and citrate combination significantly enhanced mAb production without altering product quality and suggested these compounds had a role in upregulating the protein synthetic machinery to promote protein production. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2011
Two‐phase bioreactor system for cell‐laden hydrogel assembly
Authors: Gulfam Muhammad , Lee Jong Min , Chung Bong Geun 
Published: October 2010

AbstractBottom‐up approach is a potentially useful tool for hydrogel assembly of cell‐laden individual building blocks. In this article, we assembled individual building blocks of photocrosslinkable microgels in a rapid and controlled manner. Individual building blocks of poly(ethylene glycol) (PEG) microgels with square and hexagonal shapes were fabricated by using a photolithography technique. Individual building blocks of PEG microgels were assembled on a hydrophobic mineral oil phase in a bioreactor with a magnetic stirrer. The hydrophobic mineral oil minimized the surface free energy to assemble hydrophilic PEG microgels on a two‐phase oil‐aqueous solution interface. We used the hydrophobic effect as a driving force for the hydrogel assembly. Various types of the hydrogel assembly were generated by controlling the stirring rate. As stirring speed increased, the percentage of linear, branched, and closely packed hydrogel assembly was increased. However, the percentage of random assembly was reduced by increasing stirring rate. The stirring time also played an important role in controlling the types of hydrogel assembly. The percentage of linear, branched, and closely packed hydrogel assembly was improved by increasing stirring time. Therefore, we performed directed cell‐laden hydrogel assembly using a two‐phase bioreactor system and optimized the stirring rate and time to regulate the desired types of hydrogel assembly. Furthermore, we analyzed cell viability of hydrogel linear assembly with square shapes, showing highly viable even after secondary photocrosslinking reaction. This bioreactor system‐based hydrogel assembly could be a potentially powerful approach for creating tissue microarchitectures in a three‐dimensional manner. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011
Immobilization of organophosphate hydrolase on an amyloid fibril nanoscaffold: Towards bioremediation and chemical detoxification
Authors: Raynes Jared K. , Pearce F.Grant , Meade Susie J. , Gerrard Juliet A. 
Published: October 2010

AbstractOrganophosphate hydrolase has potential as a bioremediation and chemical detoxification enzyme, but the problems of reusability and stability need to be addressed to use this enzyme on an industrial scale. Immobilizing the enzyme to a nanoscaffold may help to solve these problems. Amyloid fibrils generated from insulin and crystallin provided a novel nanoscaffold for the immobilization of organophosphate hydrolase, using glutaraldehyde as the crosslinking reagent. Electrophoretic, centrifugation, and temperature stability experiments, together with transmission electron microscopy were undertaken to verify that crosslinking had successfully occurred. The resulting fibrils remained active towards the substrate paraoxon and when immobilized to the insulin amyloid fibrils, the enzyme exhibited a significant (∼300%) increase in the relative temperature stability at 40, 45, and 50°C (as measured by comparing the initial enzyme activity to the activity remaining after heating), compared to free enzyme. This confirms that amyloid fibrils could provide a new type of nanoscaffold for enzyme immobilization. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2011
Screening for enzyme activity in turbid suspensions with scattered light
Authors: Huber Robert , Wulfhorst Helene , Maksym Lukas , Stehr Regina , Pöhnlein Martin , Jäger Gernot , Spieß Antje C. , Büchs Jochen 
Published: October 2010

AbstractNew screening techniques for improved enzyme variants in turbid media are urgently required in many industries such as the detergent and food industry. Here, a new method is presented to measure enzyme activity in different types of substrate suspensions. This method allows a semiquantitative determination of protease activity using native protein substrates. Unlike conventional techniques for measurement of enzyme activity, the BioLector technology enables online monitoring of scattered light intensity and fluorescence signals during the continuous shaking of samples in microtiter plates. The BioLector technique is hereby used to monitor the hydrolysis of an insoluble protein substrate by measuring the decrease of scattered light. The kinetic parameters for the enzyme reaction (Vmax,app and Km,app) are determined from the scattered light curves. Moreover, the influence of pH on the protease activity is investigated. The optimal pH value for protease activity was determined to be between pH 8 to 11 and the activities of five subtilisin serine proteases with variations in the amino acid sequence were compared. The presented method enables proteases from genetically modified strains to be easily characterized and compared. Moreover, this method can be applied to other enzyme systems that catalyze various reactions such as cellulose decomposition. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2011
A review of advanced small‐scale parallel bioreactor technology for accelerated process development: Current state and future need
Authors: Bareither Rachel , Pollard David 
Published: October 2010

AbstractThe pharmaceutical and biotech industries face continued pressure to reduce development costs and accelerate process development. This challenge occurs alongside the need for increased upstream experimentation to support quality by design initiatives and the pursuit of predictive models from systems biology. A small scale system enabling multiple reactions in parallel (n ≥ 20), with automated sampling and integrated to purification, would provide significant improvement (four to fivefold) to development timelines. State of the art attempts to pursue high throughput process development include shake flasks, microfluidic reactors, microtiter plates and small‐scale stirred reactors. The limitations of these systems are compared to desired criteria to mimic large scale commercial processes. The comparison shows that significant technological improvement is still required to provide automated solutions that can speed upstream process development. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2011
Interactive dimensional calibration via Internet
Authors: Gomez E. , Caja J. , Maresca P. , Barajas C. , Berzal M. 
Published: October 2010

AbstractThe purpose of this article is to show a software application made for online calibration via Internet from simple measuring instruments from the dimensional area (exterior micrometer and calliper). It also shows the educational results obtained in the teaching experiences where it has been used. The environment created makes it possible to replace real practices with virtual simulations where the student must make the proper metrological decisions and make the necessary calculations to determine the instruments' expanded uncertainty. This software has shown to be a very useful and efficient tool in training the distance learning students in the course titled: “Calibration Plan for Ensuring Quality in Industrial Laboratories,” offered by Technical University of Madrid. Furthermore, some very notable academic results have been achieved among students from two pilot groups for the Mechanical Engineering degree program. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 21: 387–399, 2013
Teaching short circuit breaking test on high‐voltage circuit breakers to undergraduate students by using MATLAB‐SIMULINK
Authors: Vahidi B. , Taherkhani M. 
Published: October 2010

AbstractThis article describes an efficient method of teaching short circuit breaking test of high‐voltage circuit breakers, to undergraduate students of power system groups in electrical engineering departments, as a part of a high‐voltage course for senior undergraduate students. The article shows how to simulate the components of testing circuit and breaker in order to teach students the test. Evaluation of the simulation over several semesters with more than 40 students is very positive in terms of their developing confidence in and understanding of short circuit breaking test on high‐voltage circuit breakers. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 21: 459–466, 2013
Outcomes following PCI in patients with previous CABG
Authors: Bundhoo Shantu S. , Kalla Manish , Anantharaman Rajaram , Morris Keith , Chase Alexander , Smith David , Anderson Richard A. , Kinnaird Tim D. 
Published: October 2010

AbstractBackground: Limited data is available to guide operators as to the optimal revascularisation strategy in patients with previous CABG representing with angina. Method: Retrospective analysis of 161 patients with prior CABG undergoing PCI in two centres between September 2005 and April 2008. Results: 161 patients (132 male, 68 ± 8years) underwent PCI at 126 ± 65 months after index CABG. Clinical presentation of recurrent ischaemia was stable in 59.7% and as an acute coronary syndrome in 40.3% of patients. Mean follow‐up after PCI was 13.5 ± 4.8 months. About 62.7% of patients underwent native vessel PCI, 32.9% had a graft only PCI, and 4.4% having a combination of both. Drug eluting stents were used in 84.9% of cases. There was one cardiac death and one case of redo CABG during follow‐up. Mean CCS angina class decreased from 2.87 to 0.67 (P < 0.0001) in the follow‐up group. About 13.6 % of all patients had a MACE at follow up. This was higher in the graft PCI group (21.6% vs. 8.9%, P = 0.048). About 12.4% of the total cohort underwent repeat PCI although 30% of these required PCI for a de‐novo lesion. TVR rate was significantly higher in patients undergoing graft PCI than native vessel PCI (15% vs. 4.9%, P = 0.031). Graft PCI was an independent predictor (HR 3.73, 1.27–10.87 [95%CI], P = 0.016) of MACE in these patients. Conclusion: PCI significantly improved angina in these patients with low overall rates of TVR. However TVR rate was significantly higher in patients undergoing graft PCI than those undergoing native vessel PCI. © 2011 Wiley‐Liss, Inc.
A nonimaging catheter for measurement of coronary artery lumen area
Authors: Hermiller James , Choy Jenny S. , Svendsen Mark , Bigelow Brian , Fouts Andrew M. , Hall Jack , Parr Kirk , Ball Michael , Sinha Anjan , Bhatt Deepak L. , Kassab Ghassan S. 
Published: October 2010

AbstractObjectives: The objective of this human pilot study was to determine the safety and the level of agreement between a novel nonimaging 2.7 Fr. catheter‐based system (LumenRECON, LR) that uses electrical conductance for measurement of lumen cross‐sectional area (CSA) with intravascular ultrasound (IVUS) and quantitative coronary angiography (QCA). Based on previous animal studies, we hypothesized the level of agreement between LR and IVUS to be 13%. Background: Accurate and reproducible vessel sizing is essential for optimal percutaneous coronary intervention (PCI). Methods: A total of 12 patients were studied to evaluate the safety, accuracy, and reproducibility of the system in comparison with IVUS and QCA. The CSA of coronary arteries was determined by IVUS, QCA, and LR in the distal, proximal, and center of a lesion during standard PCI. Results: A Bland‐Altman plot of the LR versus IVUS and QCA show a nonsignificant mean difference between the two measurements of 0.04 and 0.07 mm in diameter, respectively. The root mean square error of LR versus IVUS and QCA was 14.3 and 25.8% of the mean IVUS or QCA diameter, respectively. The mean of the difference between two LR duplicate measurements was nearly zero (0.03 mm) and the repeatability coefficient was within 8.7% of the mean of the two measurements. There were no procedural complications nor were any device‐related MACE reported within 30 days of the procedure. Conclusions: This proof of concept pilot study establishes the safety and accuracy of the conductance technology for a pivotal trial of coronary sizing. © 2011 Wiley‐Liss, Inc.
Coil closure of residual shunt after amplatzer pfo occlusion
Authors: Meier Bernhard 
Published: October 2010

AbstractA 59‐year‐old man suffered a transient ischemic attack (TIA) and was found to have a patent foramen ovale (PFO). Six months after closure with a 25 mm Amplatzer PFO occluder there was a significant residual shunt at transesophageal echocardiography (TEE). A coil was implanted and TEE 4 months later showed complete closure. © 2011 Wiley‐Liss, Inc.
Recanalization and reuse of early occluded radial artery within 6 days after previous transradial diagnostic procedure
Authors: Babunashvili Avtandil , Dundua David 
Published: October 2010

AbstractLate radial artery (RA) thrombosis occurs in 3–9% after transradial interventions. RA occlusion has made this approach unsuitable for repeat interventions and obviated the need for alternative vascular access for catheterization, e.g., left RA (with certain risk of bilateral RA occlusion) or femoral artery with its shortcomings and, sometimes, life‐threatening complications requiring surgical treatment (large groin hematoma, arterio‐venous fistula or false aneurysm, retroperitoneal hemorrhage). We demonstrate the possibility of retrograde RA recanalization, dilatation, and restoration of the RA patency within 6 days after first transradial coronary diagnostic catheterization complicated with acute RA occlusion. Thus we were able to recanalize previously occluded RA and reuse it for repeat transradial coronary interventions. © 2011 Wiley‐Liss, Inc.
All comers 5 french transfemoral percutaneous coronary intervention without sheath
Authors: Laurent Bayard Yves , Jakob Dominik , Meier Bernhard 
Published: October 2010

AbstractBackground: There is an interest to keep the arterial access site for percutaneous coronary interventions (PCI) small. Using sheaths for introduction of arterial catheters is standard. The effective outer diameter of the usual introducer sheaths is about 1.5 French (F) larger than the labeled size. Omitting the sheath affords a smaller access without loss of working lumen. Methods: PCI was performed with sheathless 5 F guiding catheters in 503 consecutive all comers via femoral access. Diagnostic assessment of the coronary arteries was performed at the beginning using 4 F diagnostic catheters with 4 F sheaths in 293 patients (58%). In the remaining patients, a sheathless 5 F Amplatz left 2 (AL2) guiding catheter was used for both diagnostic study and PCI. Data analysis was performed retrospectively using catheterization reports and hospital summaries. Results: PCI without sheath was successful in 500 of 503 patients (99%).Balloon coronary angioplasty only was performed in 155 (31%) patients, and stenting in 346 (69%). In six patients, a 6 F sheath and catheter were used additionally for various reasons. Femoral closure devices were used in 23 patients (5%). The femoral access complication rate was 5% with a major hematoma in 2%. In‐hospital mortality and Q‐wave myocardial infarction rate were both 0.2% (one patient each). A single AL2 guiding catheter with 17 exceptions and an average of 1.1 coronary guidewires per case were used. No direct complications regarding the 5 F access without a sheath were noted. Mean hospitalization time was 2.2 ± 1.5 (range 1–25) days. Conclusion: The 5 F PCI without sheath can be performed safely in all comers. Vascular access bleeding complication rates were comparable, if not lower than with the conventional 6 F interventions with or without closure devices. The savings in material are substantial. © 2011 Wiley‐Liss, Inc.
A New Class of Fluorescent Boronic Acids That Have Extraordinarily High Affinities for Diols in Aqueous Solution at Physiological pH
Authors: Cheng Yunfeng , Ni Nanting , Yang Wenqian , Wang Binghe 
Published: October 2010

AbstractThe boronic acid group is an important recognition moiety for sensor design. Herein, we report a series of isoquinolinylboronic acids that have extraordinarily high affinities for diol‐containing compounds at physiological pH. In addition, 5‐ and 8‐isoquinolinylboronic acids also showed fairly high binding affinities towards D‐glucose (Ka=42 and 46 M−1, respectively). For the first time, weak but encouraging binding of cis‐cyclohexanediol was found for these boronic acids. Such binding was coupled with significant fluorescence changes. Furthermore, 4‐ and 6‐isoquinolinylboronic acids also showed the ability to complex methyl α‐D‐glucopyranose (Ka=3 and 2 M−1, respectively).
A Time‐Resolved Fluorescence Probe for Dipeptidyl Peptidase 4 and Its Application in Inhibitor Screening
Authors: Kawaguchi Mitsuyasu , Okabe Takayoshi , Terai Takuya , Hanaoka Kenjiro , Kojima Hirotatsu , Minegishi Izumi , Nagano Tetsuo 
Published: October 2010

AbstractThe prevalence of type 2 diabetes is increasing dramatically throughout the world. Recently, dipeptidyl peptidase 4 (DPP4) was identified as a potential antidiabetes target. Many DPP4 inhibitors, such as sitagliptin and vildagliptin, have been developed and marketed, but superior therapeutic agents are still required. Therefore, we have developed new methodology for screening of DPP4 inhibitors. Absorption‐based measurements with para‐nitroaniline or fluorescence‐based measurements with the coumarin derivative 7‐amino‐4‐methylcoumarin are often used for the screening of protease inhibitors, including DPP4 inhibitors, but these strategies are not sufficiently sensitive because of interfering background absorption and fluorescence, thus giving rise to many false‐positive and false‐negative results. Therefore, we have designed and synthesised a novel DPP4 probe (Gly‐Pro‐BCD‐Tb; Gly=glycine, Pro=proline, andBCD defines the backbone of the probe comprising an aniline derivative as on/off switch, a 7‐amino‐4‐methyl‐2(1H)‐quinolinone (cs‐124) as antenna moiety, and a diethylenetriamine‐N,N,N’,N’’,N’’‐pentaacetic acid (DTPA) as chelator moiety, Tb=terbium) for time‐resolved fluorescence (TRF) measurements. TRF measurements with Gly‐Pro‐BCD‐Tb showed high sensitivity and reliability in the inhibitory assay relative to Gly‐Pro‐MCA (MCA=4‐methylcoumarin‐7‐amide), a conventional fluorescence probe for DPP4. Further, we employed our probe for high‐throughput DPP4 inhibitor screening with 3841 randomly selected compounds and found that epibestatin, an epimer of bestatin (a well‐known anticancer drug and general aminopeptidase inhibitor), showed dose‐dependent DPP4 inhibitory activity. Interestingly, bestatin did not exhibit DPP4 inhibitory activity. We believe that this screening system will be useful for the discovery of DPP4 inhibitors with novel structural scaffolds.
Heterometallic Modular Metal–Organic 3D Frameworks Assembled via New Tris‐β‐Diketonate Metalloligands: Nanoporous Materials for Anion Exchange and Scaffolding of Selected Anionic Guests
Authors: Carlucci Lucia , Ciani Gianfranco , Maggini Simona , Proserpio Davide M. , Visconti Marco 
Published: October 2010

AbstractThe modular engineering of heterometallic nanoporous metal–organic frameworks (MOFs) based on novel tris‐chelate metalloligands, prepared using the functionalised β‐diketone 1,3‐bis(4′‐cyanophenyl)‐1,3‐propanedione (HL), is described. The complexes [MIIIL3] (M=Fe3+, Co3+) and [MIIL3](NEt4) (M=Mn2+, Co2+, Zn2+, Cd2+) have been synthesised and characterised, all of which exhibit a distorted octahedral chiral structure. The presence of six exo‐oriented cyano donor groups on each complex makes it a suitable building block for networking through interactions with external metal ions. We have prepared two families of MOFs by reacting the metalloligands [MIIIL3] and [MIIL3]− with many silver salts AgX (X=NO3−, BF4−, PF6−, AsF6−, SbF6−, CF3SO3−, tosylate), specifically the [MIIIL3Ag3]X3⋅Solv and [MIIL3Ag3]X2⋅Solv network species. Very interestingly, all of these network species exhibit the same type of 3D structure and crystallise in the same trigonal space group with similar cell parameters, in spite of the different metal ions, ionic charges and X− counteranions of the silver salts. We have also succeeded in synthesising trimetallic species such as [ZnxFeyL3Ag3](ClO4)(2x+3y)⋅Solv and [ZnxCdyL3Ag3](ClO4)2⋅Solv (with x+y=1). All of the frameworks can be described as sixfold interpenetrated pcu nets, considering the Ag+ ions as simple digonal spacers. Each individual net is homochiral, containing only Δ or Λ nodes; the whole array contains three nets of type Δ and three nets of type Λ. Otherwise, taking into account the presence of weak AgC σ bonds involving the central carbon atoms of the β‐diketonate ligands of adjacent nets, the six interpenetrating pcu networks are joined into a unique non‐interpenetrated six‐connected frame with the rare acs topology. The networks contain large parallel channels of approximate hexagonal‐shaped sections that represent 37–48 % of the cell volume and include the anions and many guest solvent molecules. The guest solvent molecules can be reversibly removed by thermal activation with retention of the framework structure, which proved to be stable up to about 270 °C, as confirmed by TGA and powder XRD monitoring. The anions could be easily exchanged in single‐crystal to single‐crystal processes, thereby allowing the insertion of selected anions into the framework channels.