bluuebunny/crossref_metadata_2025_split huggingface dataset:
Stock market crash and expectations of American households
Authors: Hudomiet Péter , Kézdi Gábor , Willis Robert J. 
Published: November 2010

AbstractThis paper utilizes data on subjective probabilities to study the impact of the stock market crash of 2008 on households' expectations about the returns on the stock market index. We use data from the Health and Retirement Study that was fielded in February 2008 through February 2009. The effect of the crash is identified from the date of the interview, which is shown to be exogenous to previous stock market expectations. We estimate the effect of the crash on the population average of expected returns, the population average of the uncertainty about returns (subjective standard deviation), and the cross‐sectional heterogeneity in expected returns (disagreement). We show estimates from simple reduced‐form regressions on probability answers as well as from a more structural model that focuses on the parameters of interest and separates survey noise from relevant heterogeneity. We find a temporary increase in the population average of expectations and uncertainty right after the crash. The effect on cross‐sectional heterogeneity is more significant and longer lasting, which implies substantial long‐term increase in disagreement. The increase in disagreement is larger among the stockholders, the more informed, and those with higher cognitive capacity, and disagreement co‐moves with trading volume and volatility in the market. Copyright © 2010 John Wiley & Sons, Ltd.
Perceived Parenting Ability and Systemic Sclerosis
Authors: Poole Janet L. , Willer Kelly , Mendelson Cindy , Sanders Margaret , Skipper Betty 
Published: November 2010

AbstractObjective. The purpose of this study was to compare perceived parenting abilities in mothers with systemic sclerosis (SSc) based on the subtype of SSc and age group of their children, and to examine how the degree of pain, fatigue and functional ability relates to parenting ability.Methods. In this cross‐sectional survey, 74 mothers with SSc, who had children aged 18 years or younger living at home with them, completed a series of questionnaires online or on paper. The questionnaires included the Parent Disability Index (PDI) and surveys regarding demographic information, pain (visual analogue scale), fatigue (Multidimensional Assessment of Fatigue) and functional ability (Health Assessment Questionnaire).Results. An analysis of variance revealed that the mean age of the mother (F2,71 = 7.9; p < 0.01), mean PDI score (F2,68 = 5.4; p < 0.01) and mean pain score (F2,71 = 4.0; p = 0.02) were each associated with the age group of the children. The univariate analysis of variance results showed that dichotomized pain (F1,69 = 13.3; p < 0.01), fatigue (F1,69 = 18.9; p < 0.01) and disability (F1,69 = 9.8; p < 0.01) were each associated with the PDI score. The multivariate analysis of variance results showed that dichotomized pain (F1,68 = 7.5; p < 0.01) and fatigue (F1,68 = 12.7; p < 0.01) were associated with the PDI.Conclusion. Mothers with SSc report difficulty with parenting, which can be related to the severity of the symptoms. The findings suggest that interventions to reduce pain and fatigue may improve perceived parenting ability. Copyright © 2010 John Wiley & Sons, Ltd.
Distribution network design on the battlefield
Authors: Toyoglu Hunkar , Karasan Oya Ekin , Kara Bahar Yetis 
Published: November 2010

AbstractAmmunition, whether it be an arrow in the middle ages, a lead bullet in the 1800s, or a laser guided smart bomb today, has been the most prominent factor in determining the outcome of combat. Failing to supply the required amount of ammunition properly may lead to defeat. Our main objective in this study is to provide a decision support tool that can help plan ammunition distribution on the battlefield. We demonstrate through an extensive literature review that the existing models are not capable of handling the specifics of the problem in this study. To this end, we propose a novel three‐layer commodity‐flow location routing formulation that distributes multiple products, respects hard time windows, allows demand points to be supplied by more than one vehicle or depot, and locates facilities at two different layers. We derive several valid inequalities to speed up the solution time of our model, illustrate the performance of the model in several realistically sized scenarios, and report encouraging results. Finally, we introduce a dynamic model that designs the distribution system in consecutive time periods for the entire combat duration. © 2010 Wiley Periodicals, Inc. Naval Research Logistics 58: 188–209, 2011
Modeling and solving a multimodal transportation problem with flexible‐time and scheduled services
Authors: Moccia Luigi , Cordeau Jean‐François , Laporte Gilbert , Ropke Stefan , Valentini Maria Pia 
Published: November 2010

AbstractThis article studies a transportation problem in a multimodal network with shipment consolidation options. A freight forwarder can use a mix of flexible‐time and scheduled transportation services. Time windows are a prominent aspect of the problem. For instance, they are used to model pickup and delivery time slots. The various features of the problem can be described as elements of a digraph and their integration leads to a holistic graph representation. This allows an origin‐destination integer multi‐commodity flow formulation with nonconvex piecewise linear costs, time windows, and side constraints. Column generation algorithms are designed to compute lower bounds. These column generation algorithms are also embedded within heuristics aimed at finding feasible integer solutions. Computational results with real‐life data are presented and show the efficacy of the proposed approach. © 2010 Wiley Periodicals, Inc. NETWORKS, 2011
Iron homeostasis and responses to iron limitation in extreme acidophiles from the Ferroplasma genus
Authors: Potrykus Joanna , Jonna Venkateswara rao , Dopson Mark 
Published: November 2010

AbstractExtremely acidophilic archaea from the genus Ferroplasma inhabit iron‐rich biomining environments and are important constituents of naturally occurring microbial consortia that catalyze the production of acid mine drainage. A combined bioinformatic, transcript profiling, and proteomic approach was used to elucidate iron homeostasis mechanisms in “F. acidarmanus” Fer1 and F. acidiphilum YT. Bioinformatic analysis of the “F. acidarmanus” Fer1 genome sequence revealed genes encoding proteins hypothesized to be involved in iron‐dependent gene regulation and siderophore biosynthesis; the Fhu and NRAMP cation acquisition systems; iron storage proteins; and the SUF machinery for the biogenesis of Fe‐S clusters. A subset of homologous genes was identified on the F. acidiphilum YT chromosome by direct PCR probing. In both strains, some of the genes appeared to be regulated in a ferrous/ferric iron‐dependent manner, as indicated by RT‐PCR. A detailed gel‐based proteomics analysis of responses to iron depletion showed that a putative isochorismatase, presumably involved in siderophore biosynthesis, and the SufBCD system were upregulated under iron‐limiting conditions. No evidence was obtained for iron sparing response during iron limitation. This study constitutes the first detailed investigation of iron homeostasis in extremely acidophilic archaea.
Peripheral profiling analysis for bipolar disorder reveals markers associated with reduced cell survival
Authors: Herberth Marlis , Koethe Dagmar , Levin Yishai , Schwarz Emanuel , Krzyszton Natalia D. , Schoeffmann Stephanie , Ruh Hermelindis , Rahmoune Hassan , Kranaster Laura , Schoenborn Torsten , Leweke Markus F. , Guest Paul C. , Bahn Sabine 
Published: November 2010

AbstractLittle is known about the molecular factors that are altered in remitting bipolar disorder (BD) patients. We carried out proteome profiling of peripheral blood mononuclear cells (PBMCs) and serum from BD patients who were not experiencing mania or major depression (euthymia) compared to matched healthy controls using liquid chromatography–mass spectrometry (LC‐MSE) and Multi‐Analyte Profiling (Human Map®) platforms. This resulted in the identification of approximately 60 differentially expressed molecules involved predominantly in cell death/survival pathways. In PBMCs, this was manifested in cytoskeletal and stress response‐associated proteins, whereas most serum analytes were associated with the inflammatory response. The predicted effect of serum analytes on physiological systems was tested by treating PBMCs with serum obtained from the same patients, resulting in reduced cellular survival. These preliminary results suggest that BD patients carry a peripheral fingerprint that has detrimental effects on cell function and that could be used to distinguish BD patients from healthy controls despite being in a remission phase. It is hoped that additional studies of BD patients in the manic and depressed stages could lead to the identification of a molecular fingerprint that could be used for predicting episodic switching and for guiding treatment strategies.
Identification of host factors involved in coronavirus replication by quantitative proteomics analysis
Authors: Vogels Mijke W. , van Balkom Bas W. M. , Kaloyanova Dora V. , Batenburg Joseph J. , Heck Albert J. , Helms J. Bernd , Rottier Peter J. M. , de Haan Cornelis A. M. 
Published: November 2010

AbstractIn this study, we applied a quantitative proteomic approach, based on SILAC, to investigate the interactions of coronaviruses with the secretory pathway of the host cell, with the aim to identify host factors involved in coronavirus replication. Comparison of the protein profiles of Golgi‐enriched fractions of cells that were either mock infected or infected with mouse hepatitis virus revealed the significant depletion or enrichment of 116 proteins. Although ribosomal/nucleic acid binding proteins were enriched in the Golgi‐fractions of mouse hepatitis virus‐infected cells, proteins annotated to localize to several organelles of the secretory pathway were overrepresented among the proteins that were depleted from these fractions upon infection. We hypothesized that proteins, of which the abundance or distribution is affected by infection, are likely to be involved in the virus life cycle. Indeed, depletion of a small subset of the affected proteins by using small interfering RNAs identified several host factors involved in coronavirus infection. Transfection of small interfering RNAs targeting either C11orf59 or Golgi apparatus glycoprotein 1 resulted in increased virus replication, whereas depletion of vesicle‐trafficking protein vesicle‐trafficking protein sec22b enhanced the release of infectious progeny virus. Overexpression of these proteins, on the other hand, had a negative effect on virus replication. Overall, our study shows that the SILAC approach is a suitable tool to study host–pathogen interactions and to identify host proteins involved in virus replication.
Improvements and simplifications in in‐gel fluorescent detection of proteins using ruthenium II tris‐(bathophenanthroline disulfonate): The poor man's fluorescent detection method
Authors: Aude‐Garcia Catherine , Collin‐Faure Véronique , Luche Sylvie , Rabilloud Thierry 
Published: November 2010

AbstractFluorescent detection of proteins is a popular method of detection allying sensitivity, linearity and compatibility with mass spectrometry. Among the numerous methods described in the literature, staining with ruthenium II tris(bathophenanthroline disulfonate) is particularly cost‐effective, but slightly cumbersome owing to difficulties in the preparation of the complex and complexity of staining protocols. We describe here the modifications on both aspects that allow to perform a higher contrast staining and offer a more robust method of complex preparation, thereby maximizing the advantages of the method.
Peucedanum japonicum Thunb Inhibits High‐fat Diet Induced Obesity in Mice
Authors: Okabe Takafumi , Toda Takayoshi , Nukitrangsan Natthanan , Inafuku Masashi , Iwasaki Hironori , Oku Hirosuke 
Published: November 2010

AbstractThis study examined the antiobese activity of Peucedanum japonicum Thunb (PJT) in mice. In the first experiment, 4‐week‐old C57BL/6 mice were fed seven different diets containing 15% corn oil and 0–20% PJT powder for 4 weeks. Feeding the 10% and 20% PJT diet suppressed the body weight gain and the accumulation of abdominal and subcutaneous fats. PJT reduced serum and liver levels of triglyceride and serum levels of leptin in a dose‐dependent manner. PJT intake decreased the proportion of saturated fatty acids and increased polyunsaturated and n‐3 fatty acids in the liver. To obtain more insight into the antiobese activity of PJT, its effect on lipid absorption and enzyme activities related to lipid metabolism was studied in the second experiment. There was an increased faecal excretion of triglyceride in mice fed 5% and 10% PJT diets. Fatty acid synthase activity was decreased while carnitine palmitoyltransferase activity was increased by 10% PJT intake. These findings pointed to the usefulness of PJT for the development of a safe natural agent to reduce obesity or body weight for the first time. The rationale for the lipid lowering mechanism of PJT and the candidate compound responsible for the observations have also been discussed. Copyright © 2010 John Wiley & Sons, Ltd.
General design of the forming collar of the vertical form, fill and seal packaging machine using the finite element method
Authors: Desoki Ahmed , Morimura Hiroaki , Hagiwara Ichiro 
Published: November 2010

AbstractThe basic part of the vertical form, fill and seal packaging machine is the forming collar. The forming collar provides the shape over which packaging film is smoothly formed at high speed into a cylindrical shape. Describing the forming collar geometry and hence its design is, however, remarkably difficult. This paper presents, for the first time, a flexible methodology for calculating the complete geometry of the film rather than the usually non‐complete collar over which the film is formed. That is, a methodology to calculate the film geometry over the collar including the seam along which the film is longitudinally sealed. The film geometry is calculated such that it has minimum deformation energy. Advantages of the proposed methodology include its great flexibility to generate collars with different configurations for different needs. Among the collar generation methods reviewed, the proposed methodology is the first that can systematically consider all collar configuration parameters such as the seam configuration, general package cross‐section, flat or straight part of the collar, collar back angle, etc. A means for obtaining the exact collar geometry is also demonstrated. This enables right‐first‐time and repeatable collar production and reduces the time and cost for producing next generation packaging machines. Copyright © 2010 John Wiley & Sons, Ltd.
A two‐step cloning‐free PCR‐based method for the deletion of genes in the opportunistic pathogenic yeast Candida lusitaniae
Authors: El‐Kirat‐Chatel Sofiane , Dementhon Karine , Noël Thierry 
Published: November 2010

AbstractWe describe a new cloning‐free strategy to delete genes in the opportunistic pathogenic yeast Candida lusitaniae. We first constructed two ura3Δ strains in C. lusitaniae for their use in transformation experiments. One was deleted for the entire URA3 coding sequence; the other possessed a partial deletion within the coding region, which was used to determine the minimum amount of homology required for efficient homologous recombination by double crossing‐over of a linear DNA fragment restoring URA3 expression. This amount was estimated to 200 bp on each side of the DNA fragment. These data constituted the basis of the development of a strategy to construct DNA cassettes for gene deletion by a cloning‐free overlapping PCR method. Two cassettes were necessary in two successive transformation steps for the complete removal of a gene of interest. As an example, we report here the deletion of the LEU2 gene. The first cassette was constituted by the URA3 gene flanked by two large fragments (500 bp) homologous to the 5′ and 3′ non‐coding regions of LEU2. After transformation of an ura3Δ recipient strain and integration of the cassette at the LEU2 locus, the URA3 gene was removed by a second transformation round with a DNA cassette made by the fusion between the 5′ and 3′ non‐coding regions of the LEU2 gene. The overall procedure takes less than 2 weeks and allows the creation of a clean null mutant that retains no foreign DNA sequence integrated in its genome. Copyright © 2011 John Wiley & Sons, Ltd.
Model-based evolution of collaborative agent-based systems
Authors: Bohner Shawn A. , Graĉanin Denis , Hinchey Michael G. , Eltoweissy Mohamed 
Published: November 2010

Abstract
As demands for behaviorally sophisticated software grow, agent-based systems are increasingly being employed. Software agents are frequently applied to large, complex systems that involve interdisciplinary development teams. These complex systems have proved to be challenging to develop and evolve, even for the most competent software engineers. Taking lessons learned in other engineering disciplines such as computer and architectural engineering we investigated a model-based engineering approach called Model-Driven Architecture (MDA) to automate, whenever possible, the development and evolution of agent-based applications. In our investigation, we use the Cognitive Agent Architecture (Cougaar); one of the most mature and sophisticated collaborative agent-based architectures. MDA and Cougaar served as the primary components and implementation platform for our research. In this paper we present our approach and demonstrate how MDA is effective for producing sophisticated agent-based systems. A key challenge was found in designing a flexible meta-model framework that would accommodate both top-down domain information and bottom-up platform specific constructs, as well as the transformations and mappings between them. We employed a General Domain Application Model (GDAM) as the platform-independent model layer and General Cougaar Application Model (GCAM) layer as the platform specific model respectively. Domain-level requirements are formulated using a XML Process Definition Language (XPDL) based graphical editor and are the refined through a series of model transformations (via the underlying metamodel) to systematically generate the agent-based software system. Through an illustrative case-study, we report on the feasibility, strengths and limitations of the model-based approach as it was investigated with the Cougaar.
Formal Specification and Verification of Real-Time Systems using Graph Grammars
Authors: Michelon Leonardo , da Costa Simone André , Ribeiro Leila 
Published: November 2010

AbstractThe importance of real-time systems has enormously increased in the last decade. Application areas that typically need real-time models include railroad systems, intelligent vehicle highway systems, avionics, multimedia and telephony. To assure that such systems are correct, additionally to prove that they provide the required functionality, time constraints must be satisfied. There are already formal specification methods for real-time systems, but most of them are difficult to use by software developers, that are usually not very familiar with mathematical notation but rather specify systems using the objectoriented paradigm. In this paper we propose a formal approach to specify and analyze real-time systems that has an object-oriented flavor. This approach is based on Object-Based Graph Grammars (OBGGs), a formal description technique suitable for the specification of asynchronous distributed systems, and intuitive even for nontheoreticians. We extend OBGGs to enable explicit modeling of time constraints, and define the semantics of the specifications via transition systems. Finally, we translate timed OBGGs to Timed Automata, a formal notation that is wide spread in the area of real-time systems modeling and allows the automatic verification of properties.
Localization and mapping in urban environments using mobile Robots
Authors: Wolf Denis F. , Sukhatme Gaurav S. 
Published: November 2010

AbstractMapping is a basic capability for mobile robots. Most applications demand some level of knowledge about the environment to be accomplished. Most mapping approaches in the literature are designed to perform in small structured (indoor) environments. This paper addresses the problems of localization and mapping in large urban (outdoor) environments. Due to their complexity, lack of structure and dimensions, urban environments presents several difficulties for the mapping task. Our approach has been extensively tested and validated in realistic situations. Our experimental results include maps of several city blocks and a performance analysis of the algorithms proposed.
Normal delivery following resection of an androgen‐secreting adrenal carcinoma
Authors: Amano Toshiyasu , Imao Tetsuya , Seki Masaya , Takemae Katsurou , Yamauchi Keishi , Hata Satoru 
Published: November 2010

AbstractA 31‐year‐old female presented to a gynecological clinic complaining of amenorrhea and virilism over a 2‐month period. Blood tests revealed high serum total testosterone and free testosterone levels. A left adrenal tumor was identified following computed tomography and she was referred to our clinic where a laparoscopic left adrenalectomy was performed. The tumor weighed 98 g and the pathological diagnosis according to Weiss’ criteria was adrenocortical carcinoma. The post‐operative course was uneventful; her serum free testosterone level normalized and regular menstruation was observed 1 month post‐operatively. The patient became pregnant 1 year later, resulting in the normal delivery of a girl.
The developmental course of executive functioning in schizophrenia
Authors: Freedman David , Brown Alan S. 
Published: November 2010

AbstractExecutive dysfunction is a core feature of schizophrenia, but our understanding of the developmental course of this neuropsychological domain in the disease remains largely unexplored. A review of the research evidence points to a number of persistent debates about the course of executive functioning and its relation to illness course. A better understanding of the neurocognitive trajectories of executive functioning in schizophrenia could help identify the risk and modifying factors that influence the onset, severity and course of disease, and the chance to re‐direct or re‐shape that course and improve outcomes. To accomplish this requires assessment of the diverse and integrated nature of those abilities, and the changes over time in those abilities requires multiple instruments and techniques in order to improve the research methods and understanding of an important area of impairment in schizophrenia.
Noise exposure in convertible automobiles
Authors: Mikulec A A , Lukens S B , Jackson L E , Deyoung M N 
Published: November 2010

AbstractObjective:To quantify the noise exposure received while driving a convertible automobile with the top open, compared with the top closed.Methods:Five different convertible automobiles were driven, with the top both closed and open, and noise levels measured. The cars were tested at speeds of 88.5, 104.6 and 120.7 km/h.Results:When driving with the convertible top open, the mean noise exposure ranged from 85.3 dB at 88.5 km/h to 89.9 dB at 120.7 km/h. At the tested speeds, noise exposure increased by an average of 12.4–14.6 dB after opening the convertible top.Conclusion:Driving convertible automobiles at speeds exceeding 88.5 km/h, with the top open, may result in noise exposure levels exceeding recommended limits, especially when driving with the convertible top open for prolonged periods.