bluuebunny/crossref_metadata_2025_split huggingface dataset:
The in vitro anti‐inflammatory and anti‐angiogenic potential of a new resveratrol‐based formula (Resvega)
Authors: LANCON A , LIZARD G , LATRUFFE N 
Published: August 2012

AbstractPurpose Age‐related Macular Degeneration (AMD) is a pathology driven by inflammatory and angiogenic processes. The aim of this work was to study the in vitro anti‐inflammatory and anti‐angiogenic potential of a new resveratrol‐based formula (Resvega, Laboratoires THEA, France) on Human retinal pigment epithelium cell line ARPE‐19 after an inflammation induced by the bacterial lipopolysaccharide (LPS).Methods Human ARPE‐19 cells were subjected to inflammation by LPS (0128:B12) and co‐treated during 24 hours with Resveratrol (50,30,10 or 1µM) or the Resvega formula (50,30,10 or 1 µM expressed in Resveratrol equivalent). Cell media were collected and the levels of 6 cytokines were measured by the multiplex Cytometric Bead Array Kit (BD Bioscience) : IL‐8, IL‐1 beta, IL‐6, IL‐10, TNF alpha and IL‐12p70. Finally the level of the major angiogenic factor VEGF‐A was measured in these media by ELISA (eBioscience)Results The results showed that among the 6 cytokines only IL‐6 and IL‐8 were over‐expressed during the inflammation triggered by the LPS treatment. These two pro‐inflammatory cytokines were down‐regulated by Resveratrol and Resvega treatments by at least 50‐60% in all conditions even at the very low concetrations (1 µM). Concerning VEGF‐A levels, Resveratrol and Resvega showed a dose‐dependent inhibition. The maximum inhibition was observed for the highest concentration of Resvega (50 µM resveratrol equivalent)with a decrease by 74%.Conclusion In conclusion, we demonstrated that Resvega had an anti‐inflammatory and anti‐angiogenic effect in vitro, on the human retinal pigment epithelial cells.
Effects of bevacizumab on newborn rat retinal proteoglycans gene expression and on cell proliferation, death and differentiation, in vitro
Authors: MONTEIRO M , KREMPEL P , PORTES A , MATSUDA M , ALLODI S , SHOLL‐FRANCO A , MARQUEZINI M , PUNTAR T , MENDEZ‐OTERO R , MIGUEL N 
Published: August 2012

AbstractPurpose VEGF is an important in nervous development, promoting neurogenesis and glial cell growth. Bevacizumab (BVZ), an anti‐VEGF agent, has been extensively used for controlling pathological retinal neovascularization in adult and newborn patients, although its effect on the developing retina remains largely unknown. In this study we investigate BVZ effect on proteoglycans and on cell death, proliferation and differentiation in newborn rat retina.Methods Retinal explants of sixty 2‐day‐old Lister hooded rats were maintained in culture media with or without BVZ for 2 days. Immunohistochemical staining was assessed against proliferating cell nuclear antigen (PCNA), caspase‐3 and beclin‐1 and vimentin and glial fibrillary acidic protein (GFAP). Gene expressions for PCNA, caspase‐3, beclin‐1, vimentin, GFAP and for proteoglycans were quantified by real‐time reverse‐transcription polymerase chain reaction. Results from treatment and control groups were comparedResults No significant difference in staining intensity of PCNA, caspase‐3, beclin‐1 and GFAP or in the mRNA levels of PCNA, caspase‐3, beclin‐1, vimentin, neurocan and phosphacan was observed. However, a significant increase in vimentin levels, decrease in GFAP and in syndecan‐3 mRNA expression were observed in BVZ‐treated retinas compared to controls.Conclusion Bevacizumab did not affect cell death or proliferation in early developing rat retina but appeared to interfere with glial cell maturation (by interfering with vimentin and GFAP). It can also alter syndecan‐3 gene expression. Thus, we suggest anti‐VEGF agents be used with caution in developing retinal tissue.
Cytomegalovirus retinitis (CMVR) with and without extra‐ocular CMV (EO‐CMV) Infection – a comparative analysis of outcomes in HIV patients
Authors: GAN N , AGRAWAL R , TEOH S 
Published: August 2012

AbstractPurpose To compare the characteristics and treatment outcomes of HIV patients with cytomegalovirus retinitis (CMVR), with and without extra‐ocular CMV (EO‐CMV) infection in Singapore.Methods This is a retrospective cohort study. All HIV patients diagnosed with CMVR at the Singapore Communicable Disease Centre from January 2005 through November 2009 were included.Results 101 patients (138 eyes) were included. 92% were male, with a mean age of 48.6±10.4 years. Median follow‐up was 8 months (range 0.5‐58). 11% defaulted at a median of 6 months, and overall mortality was 24% at a median of 4 months. There were 68 patients (88 eyes) with only CMVR and 33 patients (50 eyes) with CMVR and EO‐CMV. 36% had multiple sites of EO‐CMV infection. In the EO‐CMV group, 24% developed EO‐CMV after CMVR, of which 6 were diagnosed with EO‐CMV infection within 3 months after initiation of anti‐retroviral therapy. Bilateral CMVR was significantly higher in the EO‐CMV group (52% versus 29%; p=0.03). Patients with EO‐CMV were less likely to develop immune reactivation uveitis (OR 0.8; CI 0.3‐2.4), although mortality was higher (42% versus 15%, p=0.002). Multivariate logistic regression analysis showed that a longer duration of prior anti‐CMV treatment had higher odds (OR 1.127, p=0.037) of CMVR recurrence, but no significant difference between both groups (OR 0.886, p=0.866).Conclusion EO‐CMV infection was significantly associated with increased mortality, bilateral CMVR disease and can manifest as CMV‐associated immune reconstitution inflammatory syndrome. Our results also suggest that CMVR recurrence is multi‐factorial and cessation of anti‐CMV therapy should be tailored to an individualized risk factor profile.
Macular involvement in non infectious intermediate, posterior and panuveitis
Authors: BOULADI M , CHEBIL A , LARGUECHE L , KORT F , BROUR J , TURKI F , MGHAIETH F , EL MATRI L 
Published: August 2012

AbstractPurpose To study macular involvement in non infectious intermediate, posterior and panuveitis.Methods The data of 201 eyes in 118 patients with diagnosed non infectious intermediate, posterior or panuveitis examined between January 2007 and June 2012 were retrospectively reviewed. All patients underwent complete ophthalmic examination with fluorescein angiography and optical coherence tomography (OCT) to assess macular involvement.Results Uveitis was due to behcet’s disease in 62 eyes(30.8%), sarcoidosis in 22 eyes (10.9%), VKH in 24 eyes (11.9%), sympathetic ophthalmia in 1 eye (0.5%), birdshot chorioretinopathy in 2 eyes (1%), and multiple sclerosis in 2 eyes (1%). At initial examination, 54 eyes (27.2%) demonstrated macular involvement which consisted in macular edema (ME) in 32 eyes (15.9%), serous retinal detachment in 29 eyes(14.4%), epiretinal membrane (ERM) in 8 eyes (3.9%), macular atrophy in 2 eyes (1%), vitreo‐retinal traction in 1 eye (0.5%), macular hole in 1 eye (0.5%) , and active macular chorioretinitis in 1 eye (0.5%). During follow up, 16 eyes (8%) developed macular complications including ME in 6 eyes(3%), ERM in 5 eyes (2.5%), and macular atrophy in 5 eyes (2.5%).Conclusion Macular involvement,especially macular edema, is common in non infectious intermediate, posterior and panuveitis. It may lead to irreversible visual impairment. Thus, recognition of macular morphological characteristics by performing both OCT and fluorescein angiography in uveitic disease may facilitate earlier diagnosis and the initiation of specific treatment.
Traumatic macular hole revealing a retinitis pigmentosa: a case report
Authors: BAUDOT A , KURUN S , MAALOUF T , ANGIOI K 
Published: August 2012

AbstractPurpose To report a case of traumatic macular hole revealing a retinitis pigmentosa.Methods A 33‐year old man without past medical history complained of visual loss after direct trauma of his left eye. Visual acuity was 20/50 RE and 20/400 LE. Anterior chamber examination was normal and fundus examination revealed a large macular hole associated with optic atrophy in his left eye. Right eye funduscopy showed a cystoid macular edema and optic nerve palor (RE). Many pigment deposits were present in retinal mid‐periphery of both eyes. Optical coherence tomography showed cystoid macular edema (517 microns) RE and a large macular hole (3100 microns) LE. Typical visual field defects and extinguished electroretinogram responses confirmed the diagnosis of retinitis pigmentosa.Results Macular diseases are well‐known complications of retinitis pigmentosa, especially macular edema. Vitreomacular tractions, epiretinal membranes and macular holes are less frequent. These complications may be explained by pigment epithelium disorders that may compromise ionic drainage of the retina. Causes of macular hole in retinitis pigmentosa remain uncertain. In our patient, development of a macular hole may be explained by occurrence of trauma in an eye with pre‐existent macular edema secondary to the unknown retinitis pigmentosa.Conclusion Despite macular complications of retinitis pigmentosa are relatively common, a traumatic macular hole as a revealing symptom is exceptional.
Age macular degeneration‐Alzheimer disease: relevance and interest of ophthalmologic exam in detection and follow‐up of Alzheimer disease
Authors: GONZALEZ C 
Published: August 2012

AbstractPurpose To evaluate the impact of AMD on Alzheimer disease and their in‐between visionary correlation.Methods 285 patients, 95 men, 190 women, with AMD.3 Groups A,B,C: A, 90 patients with first stage AMD (drusen, drusenoid PED, pigment, small atrophic areas);B:80 patients with predominant atrophic areas;C,115 patients with Neovascular AMD. AMD evaluation included ETDRS visual acuity (VA), complete ophthalmic examination, autofluorescence imaging (FAF), optical coherence tomography (OCT) and fluorescein (FA) angiography, and ICG when neovascular complication.AD was diagnosed and valuated by general practice doctors and /or neurologists.Results 20% patients in group A , 25% in group B, 30% in group C have mild cognitive impairment (MCI). 25% in group B, 35% in group C have early stage AD. 20% in group C have symptomatic AD. AMD ophthalmologic signs are predictive and precursor for AD. Fundus examination and even more (FAF , OCT) are useful and needed to enhance AD screening and follow‐up.Conclusion The AMD‐AD correlation allows us to improve detection, follow‐up, screening of both AMD and AD pathologies and furthermore progress in etiopathogenic knowledge and therapeutic prospects.
Bilateral macular coloboma in a patient with retinitis pigmentosa like changes
Authors: KABANAROU SA , KOUROUPAKI A , PORTALIOU DM , FERETIS E , XIROU T 
Published: August 2012

AbstractPurpose To report a case with bilateral macular coloboma and RP (retinitis pigmentosa) like changes.Methods A 46‐year‐old female presented with macular coloboma in both eyes. There was a history of strabismic amblyopia in her right eye but no other contributory medical or family history.Best corrected Snellen visual acuity (BCVA) was measured and full ophthalmic examination was performed. Visual field testing, optical coherence tomography (OCT) and international‐standard electrophysiological (ISCEV) evaluation, including pattern and full‐field electroretinography (PERG and FFERG), were also performed.Results BCVA was hand movements in the right eye and 0.5 in her left eye. Fundoscopy revealed an oval sharply‐ demarcated defect at the macula with bare sclera at its base and pigment clumping, mainly in the right eye, resembling macular coloboma. There were also severe retinal vessel attenuation and scattered retinal pigmentary changes, occasionally resembling bone spicule RPE changes, in the retinal periphery in both eyes. Optic discs were pale bilaterally. Visual field testing demonstrated bilateral scotomas. OCT revealed a crater‐like depression at the macula with atrophic neurosensory retina and absence of retinal pigment epithelium (RPE) and choroid, more extensive in her right eye, where scleral excavation was also documented. Pattern ERG as an indication of macular function was extinguished in the right eye and severely affected in her left eye. Full field ERG was extinguished bilaterally for both scotopic and photopic responses.Conclusion Bilateral macular coloboma associated with RP like changes, an abnormal electroretinography and no relevant family history, may be indicative of a developmental retinal abnormality.
Priminary observations of the effects of ACE inhibitor ramipril in patients with Stargardt's disease
Authors: REKIK R , CHARFEDDINE R 
Published: August 2012

AbstractPurpose to assess the effect of Ramipril 2% eyedrops on visual function in patients with Stargardt’s disease.Methods We conducted a prospective, interventional, open label pilot study. 12 eyes of 6 consecutive patients in whom Stargardt’s disease had been diagnosed were treated with Ramipril 2%, one drop three times a day in both eyes. Four follow‐up visits were performed over 3 months. Retinal imaging workup was performed at baseline to confirm diagnosis. During follow up, best corrected visual acuity, fundus examination, static perimetry and electroretinogram were performed to assess safety and efficacy.Results Treatment was well‐tolerated in all cases. Mean BCVA improved from 40 +/‐ 20 letters to 51 +/‐ 17. Mean sensitivity improved from 20.6 +/‐ 3.2 dB to 22.5 +/‐ 3.7dB.Conclusion Ramipril demonstrated a good local and systemic safety profile as well as efficiency in terms of BCVA improvement and retinal sensitivity in patients with Stargardt’s disease. These results encourage us to further describe the effects of Ramipril used as a NO‐donating and antioxidative drug in ophthalmology.
Commercial interest
Recurrent retinal detachment secondary to hereditary congenital collagen disease
Authors: BAMBO M , ARA M , EGEA MC , FERNANDEZ‐PEREZ S , HERRERO R , GARCIA‐MARTIN E , DE LA MATA G , SATUE M 
Published: August 2012

AbstractPurpose Hereditary progressive arthro‐ophthalmopathy, also called Stickler syndrome, is an autosomal dominant genetic disease affecting connective tissue collagen. It is considered the leading cause of inherited retinal detachment in all ages and produces multisystem manifestations such as premature arthritis, micrognathia or eye disorder.Methods A 31‐year‐old man reports hereditary progressive arthro‐ophthalmopathy. He presented degenerative myopia, congenital cataract and bilateral recurrent retinal detachments during childhood. At birth, he presented craniofacial anomalies, hearing loss and flat feet. When he was 6 years old, he suffered a retinal detachment associated with giant tear, which was treated with 20G pars plana vitrectomy and injection of silicone oil. At 22 years, the right eye presented another retinal detachment that was treated with silicone band placement, cryotherapy and C3F8 gas intravitreal injection. Two months later the retinal detachment recurred in right eye, needing 23 G pars plana vitrectomy and intravitreal silicone oil implantation.Results The patient presented several ophthalmic complications such as post‐surgical ocular hypertension and retinal tears requiring selective photocoagulation with argon laser in the right eye. Recently, genetic diagnosis was confirmed by COL11A1 gene mutation.Conclusion Development, prevention and therapeutic management of ophthalmic complications during 26 years follow‐up are presented. The importance of early diagnosis and follow‐up by a vitreoretinal surgeon are also emphasized.
Changes in chronobiological function related to retina degeneration P23H line 1 transgenic rats
Authors: PINILLA I , LAX P , FUENTES L , FERNANDEZ‐SANCHEZ L , ESQUIVA GEMA , PEREZ‐GARCIA D , RAMIRO P , SANCHEZ‐CANO A , SEGURA F , CUENCA N 
Published: August 2012

AbstractPurpose To evaluate and determinate changes in the circadian rhythms related to retinal degeneration in P23H line 1 rats.Methods Male rats homozygous for the P23H rhodopsin mutation line 1 and male SD rats were evaluated during a 12 months period. All of them were individually housed and maintained during this time under controlled humidity (60%), temperature (23 ± 1°C) and under a 12‐h light/dark cycle. Locomotor activity (LA) was monitored throughout the experiment by an intraperitoneal transmitter. Electroretinograms (ERG) were used to evaluate retinal function and retinas were evaluated by ICC looking for the degeneration pattern.Results The LA recordings from wild type and mutant animals showed no differences in the pattern of the mean waveform and the periodogram. However, a progressive decrease in the amplitude of the LA rhythm was observed in P23H rats, when compared to wild‐type animals. The nonparametric analyses of the data showed a gradual decrease in the coupling strength of the LA rhythm to environmental zeitgebers (inter‐daily stability, IS) and an increased rhythm fragmentation (intra‐daily variability, IV) in P23H rats, as compared with wild type animals. The circadian changes are related to the rate of the degeneration. In 6 months P23H line 1 rats, the only remaining photoreceptor were cones, distributed in a disrupted single layer. B‐wave amplitude was declined at this age.Conclusion Vision loss in P23H line 1 rats produces a progressive fragmentation of their circadian patterns.
Manual rhexis vs femtosecond laser assisted rhexis in cataract surgery
Authors: MOUINGA ABAYI DA , LAAYOUN J , COLAS E , ABRIEU M , LUSSATO M , RIGAL SASTOURNE JC 
Published: August 2012

AbstractPurpose To evaluate the reproducibility and safety of capsulorhexis femtosecond laser assisted.Methods Admitted patients for cataract surgery have been received into the “PERCY Military Hospital” refractive surgery unity.We have treated 11 eyes of 11 patients, on the Victus femtosecond laser platform.A control group (11 eyes of 11 patients) has been operated manually in the same center.The rhexis diameter measurement has been realized at one week postoperative,to the angiograph,as iris angiography,using the HRA OCT Spectralis.On the pictures,we measured the vertical diameter and the horizontal diameter.For the rhexis centration,three circles were drawn following the contour of the rhexis, the pupillary border,and the margin of limbus.We measured the position of rhexis center to pupillary center.Results In the laser assisted group,the mean horizontal rhexis diameter was 5.35 mm +/‐ 0.22,and the mean vertical rhexis diameter was 5.37 mm +/‐ 0.22. In the control group,the mean horizontal and vertical rhexis diameters were respectively 5.3 +/‐ 0.28,and 5.6 +/‐ 0.21.We found that 85% of femtosecond laser assisted rhexis were within 0.35 mm from the programmed diameter. In the control group,only 15% have obtained the same level of accuracy.In the laser group,the three circles have the same center,and then are concentric,tell‐tale of a rhexis good centration,unlike the manual rhexis.The femtosecond laser rhexis was circular,regular and covers the IOL’s periphery including the haptics.Conclusion The femtosecond laser has multiple benefits for cataract surgery, as the regular and circular capsule, the reduced variability in the effective lens position. The technique is reproducible and very safe.
Long‐term endothelial cell loss after iris‐fixated phakic intraocular lens for high ametropia
Authors: LECUEN N , SANDALI O , BASLI E , AMELINE B , BULLET J , PECHA F , BORDERIE V , LAROCHE L 
Published: August 2012

AbstractPurpose The aim of the study is to report and to predict long‐term endothelial cell outcomes after iris fixated phakic intraocular lens implantation for the correction of high refractive errors.Methods Retrospective, non randomized study of patient who underwent surgery between January,2000 and December,2009 in the same department in the Centre Hospitalier National d’Ophtalmologie des Quinze‐Vingts. To predict long‐term endothelial cell density, 2 joint regression models were fitted on ECD to individuals with at least 2 ECD measurement in time. Model‐predicted parameters for endothelial cell density and survival were obtained for each patient and were used in a Kaplan‐Meier method plot for predicted percentage of eyes .Results This study consisted of 78 phakic eyes including 70 myopic eyes and 8 hyperopic eyes. 73 Verisyse/Artisan and 5 foldable Artiflex were implanted. Three eyes underwent further keratorefractive surgery (Bioptic). Two eyes were lost of follow‐up.The mean follow‐up was 68 months. Recent studies reported an endothelial cell loss 5 years after surgery ranged from ‐9 to ‐14%, but we found a decrease of 25% for 18 eyes. Surgery trauma is partially involved but we also found a higher endothelial cell loss one year after surgery ( range from ‐3.5 to ‐9%).Conclusion Potential complications such as progressive endothelial cell loss and risk of dislocation are major concerns in operative decision. Close follow‐up with regular monitoring is necessary in patients with phakic intraocular lens.
Moxifloxacin superior to cefuroxime in reducing early‐phase adherence of staphylococcus epidermidis to hydrophobic intraocular lenses
Authors: BENBOUZID F , BAILLIF S , RENAUD F , DENIS P , KODJIKIAN L 
Published: August 2012

AbstractPurpose To evaluate the bacterial anti‐adhesive effect of cefuroxime and moxifloxacin on the primary‐attachment phase of S.epidermidis to acrylic intraocular lenses IOLsMethods 40 single‐piece SA30AT IOLs were used throughout the study. IOLs were divided into 5 groups. 2 groups were soaked in antibiotic solution,(moxifloxacin 0.5mg/0.1ml or cefuroxime 1mg/0.1ml),15 min before incubation in a S.epidermidis suspension 108 CFU/ml,t1.2 groups were incubated in the bacterial suspension for 15 min before being added with antibiotics,t2.The last group consisted in a control group. After 1 hour incubation at 37°C, for all groups, IOLs were removed from the test vial and rinsed before being sonicated and vortexed to remove the adhering bacteria. The resultant suspension was diluted and spread over a nutritive agar plate. Colonies were counted after 24‐hour incubation. Comparison among groups was performed using the Wald parametric statistical test. A p‐value <0.05 was considered statistically significant.Results Mean numbers of CFU/IOL was Cef‐t1:184.103[SE: 5,24 ; SD: 28,21],Cef‐t2:117.103[SE: 5,74 ; SD: 30,37], Mox‐t1:1,27.103[SE: 0,12 ; SD: 0,61], Mox‐t2:25.103[SE:1,98 ; SD: 9,72] and Ctrl:361.103[SE: 26,9 ; SD: 107,6].There was no significant difference in antibiotic time introduction concerning cefuroxime (p=0.132). Moxifloxacin was more effective when used before incubation (p<0.001). Overall, moxifloxacin was more effective than cefuroxime (p<0,001).Conclusion Both moxifloxacin and cefuroxime significantly reduced S.epidermidis adherence on IOLs.The anti‐adhesive effect was superior with moxifloxacine. Further studies are needed to confirm results on IOLs made of other biomaterials.