bluuebunny/crossref_metadata_2025_split huggingface dataset:
CuO‐Doped Mesoporous Silica Hybrid for Rapid and Sensitive Amperometric Detection of Phenolic Compounds
Authors: Liu Yiying , Lei Jianping , Ju Huangxian 
Published: August 2010

AbstractThis work constructed an amperometric biosensing platform using CuO doped mesoporous silica hybrid (CuO/SBA‐15) as a carrier. The CuO/SBA‐15 showed a pair of redox peaks of Cu2+/0. Upon immobilization of tyrosinase on the hybrid, the resulting biosensor exhibited a rapid (<0.5 s) and sensitive amperometric response to phenolic compounds under the optimized conditions. The linear response to catechol ranged from 1.2×10−9 to 3.0×10−5 M. The activation energy for enzymatic reaction was calculated to be 26.6 kJ mol−1. The apparent Michaelis–Menten constants of the enzyme electrode were estimated to be 54.6, 145, 17.0, 74.8 and 633 µM for catechol, phenol, p‐cresol, m‐cresol and dopamine hydrochloride, respectively. The metal oxide doped mesoporous silica hybrid exhibited excellent performance for construction of new biosensors.
Fabrication of Bienzymatic Glucose Biosensor Based on Novel Gold Nanoparticles‐Bacteria Cellulose Nanofibers Nanocomposite
Authors: Wang Wei , Li Hong‐Yi , Zhang De‐Wen , Jiang Jing , Cui Yi‐Ran , Qiu Shuang , Zhou Ying‐Lin , Zhang Xin‐Xiang 
Published: August 2010

AbstractAn exploration of gold nanoparticles–bacterial cellulose nanofibers (Au‐BC) nanocomposite as a platform for amperometric determination of glucose is presented. Two enzymes, glucose oxidase (GOx) and horseradish peroxidase (HRP) were immobilized in Au‐BC nanocomposite modified glassy carbon electrode at the same time. A sensitive and fast amperometric response to glucose was observed in the presence of electron mediator (HQ). Both of GOx and HRP kept their biocatalytic activities very well in Au‐BC nanocomposite. The detection limit for glucose in optimized conditions was as low as 2.3 µM with a linear range from 10 µM to 400 µM. The biosensor was successfully applied to the determination of glucose in human blood samples.
Highly Sensitive Differential Pulse Voltammetric Determination of Cd, Zn and Pb Ions in Water Samples Using Stable Carbon‐Based Mercury Thin‐Film Electrode
Authors: Ensafi Ali A. , Nazari Zahra , Fritsch Ingrid 
Published: August 2010

AbstractA new carbon‐based mercury thin‐film electrode consisting of screen‐printed carbon on a low temperature co‐fired ceramic substrate was made. Ex‐situ mercury deposition in a potassium thiocyanate solution was used. This approach an electrode with high long‐term stability (>500 measurement cycles) and reproducibility (≤2 %) for sensitive determination of ultra trace heavy metals, using differential pulse anodic stripping voltammetry. The detection limits were 0.25, 0.08 and 5.5 ng mL−1 for Cd(II), Pb(II), and Zn(II), respectively. The method was applied to the determination of the analytes in water, wastewater, lake water, and certified reference material samples with satisfactory results.
Activated Copper Cathodes as Sensors for Nitrite Analysis
Authors: Gamboa Juan C. M. , Peña Roselyn C. , Paixão Thiago R. L. C. , Lima Alex S. , Bertotti Mauro 
Published: August 2010

AbstractThe increased surface area of copper electrodes upon applying a suitable potential protocol was characterized by atomic force microscopy images. Scanning electrochemical microscopy was used to demonstrate the enhanced reactivity of the generated surface. The modified electrode showed excellent catalytic activity towards nitrite reduction in acidic medium (pH 2). This new platform was used in the development of a fast and simple voltammetric method for nitrite determination. Commercial and rainwater spiked samples were analyzed and the data showed an excellent agreement with those obtained with a reference spectrophotometric method (Griess reaction) at a confidence level of 95 % (Student’s t‐test).
Microfluidic gradient platforms for controlling cellular behavior
Authors: Chung Bong Geun , Choo Jaebum 
Published: August 2010

AbstractConcentration gradients play an important role in controlling biological and pathological processes, such as metastasis, embryogenesis, axon guidance, and wound healing. Microfluidic devices fabricated by photo‐ and soft lithography techniques can manipulate the fluidic flow and diffusion profile to create biomolecular gradients in a temporal and spatial manner. Furthermore, microfluidic devices enable the control of cell‐extracellular microenvironment interactions, including cell–cell, cell–matrix, and cell–soluble factor interaction. In this paper, we review the development of microfluidic‐based gradient devices and highlight their biological applications.
An ultra‐high temperature flow‐through capillary device for bacterial spore lysis
Authors: Hukari Kyle W. , Patel Kamlesh D. , Renzi Ronald F. , West Jay A. A. 
Published: August 2010

AbstractRapid and specific characterization of bacterial endospores is dependent on the ability to rupture the cell wall to enable analysis of the intracellular components. In particular, bacterial spores from the bacillus genus are inherently robust and very difficult to lyze or solubilize. Standard protocols for spore inactivation include chemical treatment, sonication, pressure, and thermal lysis. Although these protocols are effective for the inactivation of these agents, they are less well suited for sample preparation for analysis using proteomic and genomic approaches. To overcome this difficulty, we have designed a simple capillary device to perform thermal lysis of bacterial spores. Using this device, we were able to super heat (195°C) an ethylene glycol lysis buffer to perform rapid flow‐through rupture and solubilization of bacterial endospores. We demonstrated that the lysates from this preparation method are compatible with CGE as well as DNA amplification analysis. We further demonstrated the flow‐through lysing device could be directly coupled to a miniaturized electrophoresis instrument for integrated sample preparation and analysis. In this arrangement, we were enabled to perform sample lysis, fluorescent dye labeling, and protein electrophoresis analysis of bacterial spores in less than 10 min. The described sample preparation device is rapid, simple, inexpensive, and easily integratable with various microfluidic devices.
A miniaturized spatial temperature gradient capillary electrophoresis system with radiative heating and automated sample introduction for DNA mutation detection
Authors: Xu Zhang‐Run , Li Qi , Fan Xiao‐Feng , Zhang Hui‐Dan , Fang Jin 
Published: August 2010

AbstractA miniaturized spatial temperature gradient CE system with automated sample introduction for DNA mutation detection was established. Continuous electrokinetic sample injection was achieved by combining an automated slotted‐vial array sample introduction device to the spatial temperature gradient CE system. The temperature gradient was produced by a radiative heating system with a single graphite block heater, and the stability of the temperature gradient was investigated. The temperature variation of each measure point was 0.12–0.21% RSD (n=7) within 6 h. A 14‐cm Teflon AF‐coated silica capillary was used both as the separation channel and as the liquid‐core waveguide tube of fluorescence signal. Under a temperature gradient from 54.8 to 59.5°C, a low range control mutation standard (209 bp) was separated within 4 min with only 5.6 nL sample consumption. Automated continuous sample introducing and changing were realized with a carryover of 3.3%. Utility of the system was further demonstrated by detecting K‐ras gene mutations in paraffin tissue sections from two colorectal cancer patients.
Secondary hemophagocytic syndrome in adults: a case series of 18 patients in a single institution and a review of literature
Authors: Shabbir Munira , Lucas John , Lazarchick John , Shirai Keisuke 
Published: August 2010

AbstractHemophagocytic lymphohistiocytosis (HLH) is rare in adults and is usually fatal without treatment. We present a consecutive series of 18 adults with HLH diagnosed at our institution between 2004 and 2009. All diagnoses were confirmed by pathology. The median age at diagnosis was 56 years (range: 18–73 years), with a male: female ratio of 2:1. Patients uniformly presented with fever. Fifty‐five per cent of the patients presented with evidence of hepatomegaly or splenomegaly. All of the patients had at least a bi‐ or trilineage cytopenia. Elevated liver enzymes, hyperferritinemia, hypertriglyceridemia and hyperfibrinogenemia were seen in 50, 100, 40 and 50% of patients, respectively. The presumed causes were as follows; haematological malignancies (n = 4), post‐autologous stem cell transplant (n = 2), infection (n = 2), rheumatologic illness (n = 2), sickle cell disease (n = 1), post‐orthotopic liver transplant (n = 1) and idiopathic (n = 3). The median time from suspicion to diagnosis was 5 days (1–27 days). Corticosteroids and/or cyclosporine were the most frequently used treatment regimen. Other agents used were etoposide, IVIG, cyclophosphamide and chemotherapy. The mortality rate was 72%, with multi‐system organ failure being the most common cause of death. Median survival time from diagnosis was 35 days. Six patients are alive to date. In a univariate analysis, the presence of fever was the only factor that was statistically significant for predicting a poor prognosis (early mortality) (p = 0.05). In conclusion, a high index of suspicion is the critical factor for early diagnosis. Early treatment with immunosuppressant is warranted, and a thorough diagnostic evaluation to identify the underlying cause should be undertaken. Copyright © 2010 John Wiley & Sons, Ltd.
Chitosan‐based gene delivery vectors targeted to the peripheral nervous system
Authors: Oliveira Hugo , Pires Liliana R. , Fernandez Ramon , Martins M. Cristina L. , Simões Sérgio , Pêgo Ana P. 
Published: August 2010

AbstractA non‐toxic, targeted, simple and efficient system that can specifically transfect peripheral sensorial neurons can pave the way towards the development of new therapeutics for the treatment of peripheral neuropathies. In this study chitosan (CH), a biodegradable polymer, was used as the starting material in the design of a multicomponent vector targeted to the peripheral nervous system (PNS). Polycation‐DNA complexes were optimized using imidazole‐ and thiol‐grafted CH (CHimiSH), in order to increase transfection efficiency and allow the formation of ligand conjugated nanocomplexes, respectively. The 50 kDa non‐toxic fragment from the tetanus toxin (HC), shown to interact specifically with peripheral neurons and undergo retrograde transport, was grafted to the binary complex via a bi‐functional poly(ethylene glycol) (HC‐PEG) reactive for the thiol moieties present in the complex surface. The targeting of the developed nanocomplexes was assessed by means of internalization and transfection studies in the ND7/23 (neuronal) vs. NIH 3T3 (fibroblast) cell lines. Targeted transfection was further confirmed in dorsal root ganglion dissociated primary cultures. A versatile, multi‐component nanoparticle system that successfully targets and transfects neuronal cell lines, as well as dorsal root ganglia (DRG) primary neuron cultures was obtained for the 1.0 (w/w) HC‐PEG/DNA formulation. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Encapsulation of ePTFE in prevascularized collagen leads to peri‐implant vascularization with reduced inflammation
Authors: Gruionu Gabriel , Stone Alice L. , Schwartz Mark A. , Hoying James B. , Williams Stuart K. 
Published: August 2010

AbstractDuring the typical healing response to an implanted biomaterial, vascular‐rich granulation tissue forms around the implant and later resolves into a relatively avascular, fibrous capsule. We have previously shown that a microvascular construct (MVC) consisting of isolated microvessel fragments suspended in a collagen I gel forms a persistent microcirculation in lieu of avascular scar when implanted. The current study evaluated the potential for microvascular constructs to maintain a vascularized tissue environment around an implanted biomaterial. An analysis of the peri‐implant tissue around bare expanded polytetrafluoroethylene (ePTFE), ePTFE embedded within a microvascular construct, or ePTFE embedded within collagen alone revealed that the presence of the MVC, but not collagen alone, promoted vascular densities comparable to that of the granulation tissue formed around bare ePTFE. The vessels within the microvascular construct surrounding the ePTFE were perfusion competent, as determined by India ink perfusion casting, and extended into the interstices of the polymer. In contrast to bare ePTFE, the presence of the MVC or collagen alone significantly reduced the number of activated macrophages in association with ePTFE. Similar results were observed for ePTFE modified to increase cellularity and prevent the formation of an avascular scar. The microvascular construct may prove effective in forming vascularized tissue environments and limiting the number of activated macrophages around implanted polymers thereby leading to effective implant incorporation. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Anaphylactoid‐like reactions in a patient with HyperLp(a)lipidemia undergoing LDL apheresis with dextran sulfate adsorption and herbal therapy with the spice turmeric
Authors: Adamski Jill , Jamensky Lita , Ross Joyce , Siegel Don L. , Sachais Bruce S. 
Published: August 2010

AbstractElevated Lipoprotein (a) (Lp(a)) levels are associated with atherosclerosis and are independent risk factors for coronary artery disease and stroke [Ariyo et al., N Engl J Med 2003;349:2108–2115; Price et al., Atherosclerosis 2001;157:241–249]. Low‐density lipoprotein (LDL)‐apheresis is the most effective therapy for reducing Lp(a) levels [Parker, Chem Phys Lipids 1994;67–68:331–338; Stefanutti et al., Transfus Apher Sci 2010;42:21–26]. Dextran sulfate‐cellulose adsorption (Liposorber®) removes both LDL and Lp(a) particles with minimal effect on high‐density lipoprotein levels. During the procedure, high levels of bradykinin are generated as the kallikrein‐kinin system is activated by contact with the negatively charged dextran‐sulfate cellulose [Krieter et al., Artif Organs 2005;29:47–52]. Bradykinin is a potent vasodilator and a substrate of the angiotension converting enzyme (ACE). ACE inhibitors are contraindicated for apheresis procedures because these drugs prevent bradykinin degradation, which causes anaphylatoid reactions characterized by hypotension, bradycardia, dyspnea, and flushing [Owen and Brecher, Transfusion 1994;34:891–894]. Turmeric is a yellow spice that is used as an herbal remedy to treat a myriad of conditions ranging from abdominal pain to pulmonary infections. Scientific investigations of the ethnomedicinal properties of curcumin, the major derivative of turmeric, suggest that this compound has anti‐inflammatory, antioxidant, and antineoplastic properties [Lobo et al., J Pharm Pharmacol 2009;61:13–21]. We report a case of a patient undergoing Liposorber® therapy for treatment of hyperLp(a)lipidemia who had three episodes of anaphylactoid‐like reactions after starting therapy with the spice turmeric. J. Clin. Apheresis, 2010. © 2010 Wiley‐Liss, Inc.
Optimizing stem cell culture
Authors: van der Sanden Boudewijn , Dhobb Mehdi , Berger François , Wion Didier 
Published: August 2010

AbstractStem cells always balance between self‐renewal and differentiation. Hence, stem cell culture parameters are critical and need to be continuously refined according to progress in our stem cell biology understanding and the latest technological developments. In the past few years, major efforts have been made to define more precisely the medium composition in which stem cells grow or differentiate. This led to the progressive replacement of ill‐defined additives such as serum or feeder cell layers by recombinant cytokines or growth factors. Another example is the control of the oxygen pressure. For many years cell cultures have been done under atmospheric oxygen pressure which is much higher than the one experienced by stem cells in vivo. A consequence of cell metabolism is that cell culture conditions are constantly changing. Therefore, the development of high sensitive monitoring processes and control algorithms is required for ensuring cell culture medium homeostasis. Stem cells also sense the physical constraints of their microenvironment. Rigidity, stiffness, and geometry of the culture substrate influence stem cell fate. Hence, nanotopography is probably as important as medium formulation in the optimization of stem cell culture conditions. Recent advances include the development of synthetic bioinformative substrates designed at the micro‐ and nanoscale level. On going research in many different fields including stem cell biology, nanotechnology, and bioengineering suggest that our current way to culture cells in Petri dish or flasks will soon be outdated as flying across the Atlantic Ocean in the Lindbergh's plane. J. Cell. Biochem. 111: 801–807, 2010. © 2010 Wiley‐Liss, Inc.
CABS‐NMR—De novo tool for rapid global fold determination from chemical shifts, residual dipolar couplings and sparse methyl‐methyl noes
Authors: Latek Dorota , Kolinski Andrzej 
Published: August 2010

AbstractRecent development of nuclear magnetic resonance (NMR) techniques provided new types of structural restraints that can be successfully used in fast and low‐cost global protein fold determination. Here, we present CABS‐NMR, an efficient protein modeling tool, which takes advantage of such structural restraints. The restraints are converted from original NMR data to fit the coarse grained protein representation of the C‐Alpha‐Beta‐Side‐group (CABS) algorithm. CABS is a Monte Carlo search algorithm that uses a knowledge‐based force field. Its versatile structure enables a variety of protein‐modeling protocols, including purely de novo folding, folding guided by restraints derived from template structures or, structure assembly based on experimental data. In particular, CABS‐NMR uses the distance and angular restraints set derived from various NMR experiments. This new modeling technique was successfully tested in structure determination of 10 globular proteins of size up to 216 residues, for which sparse NMR data were available. Additional detailed analysis was performed for a S100A1 protein. Namely, we successfully predicted Nuclear Overhauser Effect signals on the basis of low‐energy structures obtained from chemical shifts by CABS‐NMR. It has been observed that utility of chemical shifts and other types of experimental data (i.e. residual dipolar couplings and methyl‐methyl Nuclear Overhauser Effect signals) in the presented modeling pipeline depends mainly on size of a protein and complexity of its topology. In this work, we have provided tools for either post‐experiment processing of various kinds of NMR data or fast and low‐cost structural analysis in the still challenging field of new fold predictions. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2011
A cavernous hemangioma of the thyroid gland: First documentation by ultrasound of a rare pathology
Authors: Gutzeit Andreas , Stuckmann Gerd , Tosoni Ivo , Erdin Dieter , Binkert Christoph A. 
Published: August 2010

AbstractHemangioma of the thyroid gland is an extremely rare condition. We report a case of a primary cavernous hemangioma in the left lobe of the thyroid gland in an 84‐year‐old woman. Ultrasound examination of the lesion showed an inhomogeneous and hypoechoic nodule that was well demarcated from the rest of the left lobe and hypovascular on color Doppler ultrasound. Ultrasound‐guided fine‐needle aspiration was performed. The cytologic differential diagnosis included an anaplastic carcinoma of the thyroid gland. After hemithyroidectomy, the final diagnosis was a benign hemangioma of the thyroid gland. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound, 2011.
Trichloroisocyanuric acid‐catalyzed reaction of indoles: An expeditious synthesis of bis‐indolyl, tris‐indolyl, di(bis‐indolyl), tri(bis‐indolyl), and tetra(bis‐indolyl)methane under solid‐state conditions
Authors: Veisi Hojat , Gholbedaghi Reza , Malakootikhah Javad , Sedrpoushan Alireza , Maleki Behrooz , Kordestani Davood 
Published: August 2010

Abstract
magnified image Trichloroisocyanuric acid is found to be an efficient catalyst for the electrophilic substitution reaction of indole with aldehydes/ketones to afford the corresponding bis‐indolyl, tris‐indolyl, di(bis‐indolyl), tri(bis‐indolyl), and tetra(bis‐indolyl)methanes under solid‐state conditions by pulverization‐activation method at room temperature with excellent yields. The remarkable features of this new procedure are high conversions, shorter reaction times, cleaner reaction profiles, and simple experimental and work‐up procedures. J. Heterocyclic Chem., (2010).
Persistent neural activity regulates Arc/Arg3.1 transcription in the dentate gyrus
Authors: Toyoda Takeshi , Nomura Hiroshi , Hashikawa Koichi , Nonaka Ayako , Matsuki Norio 
Published: August 2010

AbstractThe activity‐regulated cytoskeleton‐associated gene (Arc, also known as Arg3.1) is an effector immediate‐early gene rapidly induced by strong neural activity. Although a number of studies have revealed significant functions of Arc and Arc has come into widespread use as a neural activity marker in behavioral studies, the mechanisms regulating Arc transcription remain unclear. Here, we examined the conditions of Arc transcription in acute slices of dentate gyrus. Surprisingly, kainic acid (1 μM to 10 mM) application to slices did not induce Arc transcription, although intraperitoneal injection of kainic acid (20 mg/kg) induced robust Arc transcription. No types of high‐frequency stimulation examined induced Arc transcription in acute slices. These findings indicate that Arc transcription is dramatically suppressed in acute slices of the dentate gyrus, in which background neural activity is markedly reduced. Burst stimulation increased the number of Arc‐expressing cells in the presence of picrotoxin, in which excitation was maintained even after the end of stimulation. Moreover, the involvement of background neural activity in Arc transcription was tested by application of carbachol, a muscarinic receptor agonist. Carbachol also increased the number of Arc‐expressing cells, which was blocked by atropine, a muscarinic receptor antagonist. Taken together, these findings suggest that persistent background activity is critical for Arc transcription. © 2010 Wiley‐Liss, Inc.
Characterization of an unusual cold shock protein from Staphylococcus aureus
Authors: Chanda Palas K. , Bandhu Amitava , Jana Biswanath , Mondal Rajkrishna , Ganguly Tridib , Sau Keya , Lee Chia Y. , Chakrabarti Gopal , Sau Subrata 
Published: August 2010

AbstractOf the three cold shock proteins expressed by Staphylococcus aureus, CspC is induced poorly by cold but strongly by various antibiotics and toxic chemicals. Using a purified CspC, here we demonstrate that it exists as a monomer in solution, possesses primarily β‐sheets, and bears substantial structural similarity with other bacterial Csps. Aggregation of CspC was initiated rapidly at temperatures above 40 °C, whereas, the Gibbs free energy of stabilization of CspC at 0 M GdmCl was estimated to be +1.6 kcal mol–1, indicating a less stable protein. Surprisingly, CspC showed stable binding with ssDNA carrying a stretch of more than three thymine bases and binding with such ssDNA had not only stabilized CspC against proteolytic degradation but also quenched the fluorescence intensity from its exposed Trp residue. Analysis of quenching data indicates that each CspC molecule binds with ∼5 contiguous thymine bases of the above ssDNA and binding is cooperative in nature. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)