bluuebunny/crossref_metadata_2025_split huggingface dataset:
Understanding the heterogeneity in liver hepatocellular carcinoma with a special focus on malignant cell through single-cell analysis
Authors: Bao Mengmeng , Wu Anshi 
Published: June 2024

Abstract
Introduction
Hepatocellular carcinoma (HCC) is the most common form of liver cancer globally and remains a major cause of cancer-related deaths. HCC exhibits significant intra-tumoral and interpatient heterogeneity, impacting treatment efficacy and patient prognosis.

Methods
We acquired transcriptome data from the TCGA and ICGC databases, as well as liver cancer chip data from the GEO database, and processed the data for subsequent analysis. We also obtained single cell data from the GEO database and performed data analysis using the Seurat package. To further investigate epithelial cell subgroups and their copy number variations, we used the Seurat workflow for subgroup classification and the InferCNV software for CNV analysis, utilizing endothelial cells as a reference. Pseudo-time analysis and transcription factor analysis of epithelial cells were performed using the monocle2 and SCENIC software, respectively. To assess intercellular communication, we employed the CellChat package to identify potential ligand-receptor interactions. We also analyzed gene expression differences and conducted enrichment analysis using the limma and clusterProfiler packages. Additionally, we established tumor-related risk characteristics using Cox analysis and Lasso regression, and predicted immunotherapy response using various datasets.

Results
The samples were classified into 23 clusters, with malignant epithelial cells being the majority. Trajectory analysis revealed the differentiation states of the malignant epithelial cells, with cluster 1 being in the terminal state. Functional analysis revealed higher aggressiveness and epithelial-mesenchymal transition (EMT) scores in cluster 1, indicating a higher propensity for metastasis. RBP4+ tumor cells were highly enriched with hypoxia process and intensive cell-to-cell communication. A prognostic model was established, and immune infiltration analysis showed increased infiltration in the high-risk group. TP53 demonstrated significant differences in mutation rate between the two risk groups. Validation analysis confirmed the up-regulation of model genes, including AKR1B10, ARL6IP4, ATP6V0B, and BSG in tumor tissues.

Conclusion
A prognostic model was established based on HCC malignant cell associated gene signature, displaying decent prognosis guiding effectiveness in the multiple cohorts. The study provided comprehensive insights into the heterogeneity and potential therapeutic targets of LIHC.
Engaging with Conspiracy Believers
Authors: Douglas Karen M. , Sutton Robbie M. , Biddlestone Mikey , Green Ricky , Toribio-Flórez Daniel 
Published: June 2024

AbstractConspiracy theories abound in social and political discourse, believed by millions of people around the world. In this article, we highlight when it is important to engage with people who believe in conspiracy theories and review recent literature highlighting how best to do so. We first summarise research on the potentially damaging consequences of conspiracy beliefs for individuals, including consequences related to psychopathology. We also focus on the consequences for groups, and societies, and the importance of understanding and addressing conspiracy beliefs. We then review recent literature on how to engage with people who believe in conspiracy theories, specifically with the goal to reduce susceptibility to conspiracy theories and other types of misinformation. We focus on interpersonal strategies to communicate with individuals who believe in conspiracy theories, and large-scale strategies designed to reduce conspiracy beliefs within broader communities.
Treatment of crude oil-polluted water using CoFe2O4-doped mango (Mangifera indica) seed shell composite
Authors: Asadu Christian O. , Ujah Chika Oliver , Ekwueme Benjamin Nnamdi , Ezema Chinonso Anthony , Onah Thomas O. , Makhkamov Trobjon , Aka Christian Chikezie , Ugwele Franklin O. , Onu Chijioke Elijah 
Published: June 2024

AbstractThis study investigates the potential of cobalt ferrite-doped mango seed shell (CoFe2O4-MSS) as an innovative and eco-friendly approach for the treatment of crude oil-polluted water. CoFe2O4-MSS was synthesized by impregnating carbonized mango seed shell with cobalt ferrite nanoparticles through thermal precipitation. The study systematically evaluated the adsorption capacity, kinetics, and isotherm behavior of the developed material using standard equations. Experimental results demonstrate the effectiveness of the cobalt ferrite-doped mango seed shell in adsorbing crude oil components, with high removal efficiency of 98.3% at 80 °C after 50 min. The crystallite sizes of raw mango seed shell and CoFe2O4-MSS are 31.8 nm and 21.3 nm, respectively. The calculated adsorption capacity stood at 55.50 mg/g, the Brunauer–Emmett–Teller surface area of CoFe2O4-MSS was 1007.4m2/g with a porosity distribution of 1.685 $$\eta$$
η
while the volume and pore diameter are 3.104m3/g and 7.212 nm, respectively. FTIR analysis revealed the presence of aliphatic, aromatic, and silicon compounds. The isotherm data matched well with Langmuir isotherm model while kinetic data fitted well with Bhattacharya–Venkobachar model. The unique properties of cobalt ferrite, a magnetic and iron-based material, combined with the abundant and biodegradable nature of mango seed shells, make this composite an attractive adsorbent for removing crude oil contaminants. This research contributes to the development of sustainable and cost-effective solutions for addressing environmental challenges posed by crude oil pollution. Also, this research has contributed immensely to the sustainable development goals of the united nation (UN-SDG) regarding environmental protection.
No Such Thing as Containment? Gene Drives for Conservation and the (Im)possibility of an Island
Authors: Boersma Keje , Bovenkerk Bernice , Ludwig David 
Published: June 2024

AbstractThis article explores the use of islands as tools of geographical and intellectual containment - or what we call “islanding” - in the scientific and policy literature about gene drive technologies in conservation. In the first part of the article, we explore the narrative of contained gene drive use on islands and discuss how it juggles notions of localness and localization of gene drives and their (test) releases. We question the possibility and narrative of containing the spread of gene drives technologically or geographically, and argue that the gene drives for conservation literature strategically combines contradictory and reductive understandings of islands and containment. The second part of the article is devoted to reflection on nonlocal concerns about gene drives and the possibility of local gene drive decisions. We argue that attempts to legitimize local gene drives through local decision-making evade normative concerns about their nonlocalizability and risk instrumentalizing local communities for nonlocal agendas. Our overarching conceptual aim is therefore to open up a domain of thinking around the possibility of demarcation in our world – of our political, normative decisions, and of our reality – and to argue for the vital importance of reflection on this possibility in technological decision-making.
Higher early than late-season residue load of pesticides in honey bee bread in Slovakia
Authors: Staroň Martin , Alkassab Abdulrahim T. , Sabo Rastislav , Demková Lenka , Valenčáková Alexandra , Michalko Miloslav , Legáth Jaroslav , Pistorius Jens , Sabová Lucia 
Published: June 2024

AbstractBees are often exposed to pesticide residues during their foraging trips in agricultural landscapes. The analysis of in-hive stored pollen reflects the spectrum of visited plants and can be almost used to link the exposure to pesticides.In the current study, bee bread samples were collected in May and July from 17 sites located in southern Slovakia. Samples were analysed using a multi-residue pesticide analysis method for a broad spectrum of active substances and microscopic for pollen identification.Our results revealed a bee bread contamination with 19 different active substances, with fungicides being predominant. Sixteen of them are authorized in the EU, but chlorpyrifos, chlorpyrifos-methyl, and chloridazon are not. The highest concentrations for pendimethalin (1400 µg/kg), fluazifop-butyl (640 µg/kg), fenpropidin (520 µg/kg), fluopyram (130 µg/kg), and difenoconazole (95 µg/kg) were detected. The total residue load in bee bread sampled in the early season (May) was significantly higher than in the late season (July). The mean residue load of insecticides analysed in July comprised 46% of May’s load, which is alarming due to the importance of bee bread in the diet for winter-rearing bees. Moreover, results from both sampling periods showed that fungicides were positively associated with plant families Apiaceae and Papaveraceae and herbicides with Aceraceae, Salicaceae, and Brassica-type/Brassicaceae.Hence, bee bread can be considered a suitable matrix and a good bio-indicator reflecting honey bee exposure to pesticides over the season.
Impact of pruritus in patients undergoing hemodialysis in Italy: a patient-based survey
Authors: Santoro Antonio , Gibertoni Dino , Ambrosini Andrea , De Ferrari Maria Elisabetta , Vanacore Giuseppe 
Published: June 2024

Abstract
Background
Itching is an annoying symptom which afflicts patients with chronic renal failure. We aimed to assess the impact and patient’s perception and experience of itching in the dialysis population in Italy.

Methods
A questionnaire was developed by the National Hemodialysis and Dialysis Association of Italy (ANED) and administered to 996 hemodialysis recipients across 153 Italian dialysis centers. The main outcomes investigated by the questionnaire were patients’ satisfaction on answers regarding the nature of itching; continuing to talk about itching with the nephrologist; beliefs about resolution of itching.

Results
A total of 1903 patients from 153 centers responded to the questionnaire. Patients who responded had a mean age of 67.9 ± 13.8 years (63.9% male) and were stratified by itch discomfort graded as mild (35.9%), moderate (29.6%), and severe (34.4%). Severe itching disrupted patients' daily lives, strained their relationships, caused anxiety, and diminished their quality of life. Patients with severe itch were more likely to talk about it with dialysis staff and to undertake dermatological visits. However, only 18.0% of patients reporting severe itching found the clinicians' responses satisfactory, compared to 49.1% of mild itch patients. Those who continued talking to nephrologists about itching received more satisfactory response. However, 40.8% believed itching could not be alleviated and were less likely to discuss it with nephrologists.

Conclusions
There is an intricate relationship between the severity of itching, patient perceptions, and healthcare communication among hemodialysis patients. A substantial proportion of patients experiencing severe itching expressed feelings of resignation, highlighting the pressing need for enhanced clinician-patient communication.

Graphical Abstract
Petrology and association of rare earth elements in magmatically altered high-ash coal of Indian origin
Authors: Banerjee Riya , Chakladar Saswati , Kumar Alok , Chattopadhyay Shyamal Kumar , Chakravarty Sanchita 
Published: June 2024

AbstractThe extraction of valuables from waste has gained momentum. Thermal influence alters both the organic and inorganic components of coal. Insufficient knowledge on the association of rare earth elements (REEs) with the parent matrix of thermally altered high-ash coals (63% ash) limits the potential for such coals being utilized for isolation of valuables. In this study, we analyzed the distribution and occurrence modes of REEs within a magmatically altered high-ash coal via nine-step sequential extraction, combining Tessier and BCR methods. The total concentration of REEs in the coal sample, on whole coal basis, was found to be 820 ppm, which is significantly higher than the world average. Major mineral oxides were deduced to be those of Si, Fe, Al, Ca, Mg, and Ti. Sequential extraction confirmed that about 66% of HREE and 25% of LREE were included in the residual fraction. LREEs were concluded to be primarily in ionic form, whereas HREEs were speculated to be associated with the TiO2 phase. XRD analyses showed that thermal alteration affected the dolomite phase specifically, which selectively got removed where carbonate-bound elements were assessed. Petrographic analysis supported the magmatic influence and demonstrated the presence of mosaic structures and pores containing unfused vitrinite, with a reflectance value of 3.6. To summarize, the present study pertaining to delineation of association of valuables in high-ash heat-altered coals from an Eastern coalfield in India can potentially open up new avenues for utilizing such coals, which are otherwise considered waste.
Analyzing Healthcare Processes with Incremental Process Discovery: Practical Insights from a Real-World Application
Authors: Schuster Daniel , Benevento Elisabetta , Aloini Davide , van der Aalst Wil M. P. 
Published: June 2024

Abstract
Most process mining techniques are primarily automated, meaning that process analysts input information and receive output. As a result, process mining techniques function like black boxes with limited interaction options for analysts, such as simple sliders for filtering infrequent behavior. Recent research tries to break these black boxes by allowing process analysts to provide domain knowledge and guidance to process mining techniques, i.e., hybrid intelligence. Especially, in process discovery—a critical type of process mining—interactive approaches emerged. However, little research has investigated the practical application of such interactive approaches. This paper presents a case study focusing on using incremental and interactive process discovery techniques in the healthcare domain. Though healthcare presents unique challenges, such as high process execution variability and poor data quality, our case study demonstrates that an interactive process mining approach can effectively address these challenges.

Graphical Abstract
Control of coptis root rot by combination of Bacillus cereus isolate Y9 and other antagonistic microorganisms
Authors: Mei Pengying , Dou Tao , Song Xuhong , Li Longyun 
Published: June 2024

AbstractRoot rot is a destructive soil-borne disease of Coptis chinensis, which depends on chemical control at present, and more attention should be paid to biocontrol of disease. In the present research, isolate Y9 isolated from healthy root samples of Coptis, was identified as Bacillus cereus. Further screening and pot experiments showed that B. cereus isolate Y9 inhibited the growth of the main causal agents of coptis root rot disease (Fusarium solani and F. avenaceum) and seven other phytopathogenic fungi. The application of B. cereus isolate Y9 and compatible Trichoderma harzianum, T. atroviride and B. amyloliquefaciens, singly and in combination were found to be effective against Fusarium root rot in vitro and in field experiments. In field experiments, combinations of T. harzianum + B. amyloliquefaciens + Y9 (HYJ, in ratio of 1:1:1) showed the highest control efficacy of 63.85%, which was higher than the expected value (53.18%), indicating synergistic effect on the control of coptis root rot. Therefore, B. cereus isolate Y9 may be a potential biological control agent, and combined use with T. harzianum and B. amyloquefaciens offered even greater potential. The long-term effects of isolate B. cereus Y9 and its combinations on C. chinensis should be assessed in different locations and seasons in the future.
Alternating direction method of multipliers based distributed energy scheduling of grid connected microgrid by considering the demand response
Authors: Pothireddy Krishna Mohan Reddy , Vuddanti Sandeep 
Published: June 2024

AbstractGlobal warming, environmental degradation, clean energy production, intermittent, volatile, and unpredictable renewable energy sources (RES’s), occasional peak demand on the system necessitates energy management (EM). Demand response (DR) programs in the distribution network can be seen as one of the foundation stones in the future of EM. This article illustrates the need for EM using DR, its benefits, types of loads, clustering techniques, price-based demand response (PBDR) etc. To accomplish the EM goals and to attain the economic benefit, DR employs peak shifting, peak clipping, valley filling and load growth. However, the accumulation of large loads at low electricity prices creates local peaks, this phenomenon is referred to as payback or rebound effect (RE). The occurrence of RE at low price zone heightens the volatility of market clearing price (MCP) and the operational cost of the microgrid. Inherently, the scheduled inelastic consumers at low price zone suffer from increased MCP and therefore, the total consumer tariff (TCT). The occurrence of RE depends on the load curve, peak to average ratio, electricity price and the percentage of interruptible loads present in the system. Unclear pricing methods impede the participation of customers in DR events. Moreover, majority of techniques presented in literature are of centralized frameworks that needs complex communication technologies. To fill these glitches the proposed work uses a simple distributed scheduling approach based on alternating direction method of multipliers (ADMM) to alleviate the energy management using an IEEE-18 bus system. The load factor increases from 0.79 to 0.83. Using DR lowers the peak power demand on the MG from 82 to 78 kW without compromising customer comfort or satisfaction. The TCT was lowered from scenario 1 to scenario 4 from 3058 to 2254 euros. The system's average demand dropped from 65.54 kW to 64.8 kW. IEEE-33 bus system was considered to assess the impact of RE on the MCP and TCT. Additionally, the marginal generator provides 72.51 kW of electricity in sub case 3 and 166.26 kW of power in sub case 2. Due to a decrease in power dispatch from the marginal generator, TCT increased from sub case 2 to sun case 3 by 11,046.41 rupees to 12,912.75 rupees. In contrast, TOC decreased from 6495.45 rupees to 6150.75 rupees from sub case 2 to sun case 3.
Unravelling the economic impact of climate change in Vietnam's Mekong River Delta and Southeast region
Authors: Hai Do Huu , Nam Vu Quynh , Samadhiya Ashutosh , Kumar Anil , Gupta Sumit , Jagtap Sandeep 
Published: June 2024

AbstractVietnam faces a heightened susceptibility to natural disasters stemming from climate change, yet the impact of these disasters varies markedly across its regions. Consequently, it is imperative to scrutinize the climatic factors affecting the regional economy, with a particular dearth of research addressing the nexus between natural threats and the economies of the Mekong River Delta and the Southeast region of Vietnam. The primary objective is to investigate how temperature fluctuations, rising sea levels, storms, and forest fires can detrimentally affect agriculture, forestry, and infrastructure in these regions. The present research uses the secondary data from Vietnam's General Statistics Office and the German Agency for International Cooperation. Employing quantitative analysis, the study endeavours to assess and compare the influence of climate change elements on the economic trajectories of the Mekong River Delta and the Southeast region. The findings reveal that the Mekong River Delta is disproportionately impacted by economic losses attributed to rising sea levels when juxtaposed with other regions in Vietnam. Firstly, the escalating sea levels significantly jeopardize residential and agricultural land in the Mekong River Delta (1). Secondly, while wildfires inflict damage on forestry in both the Mekong River Delta and the Southeast area, their overall impact is deemed negligible (2). Thirdly, the study ascertains that houses in the Mekong River Delta and the Southeast region remain relatively unscathed by storms (3). However, it is noted that specific storms have inflicted substantial damage on both regions (4). In light of these findings, future research should delve deeper into the analysis of storm risks in the Mekong River Delta and the Southeast region. Such insights are crucial for enhancing our understanding of the vulnerabilities and devising effective strategies to mitigate the economic repercussions of climate change in these pivotal areas.
Advanced fault detection technique of three phase induction motor: comprehensive review
Authors: Bahgat B. Hafez , Elhay Enas A. , Elkholy Mahmoud M. 
Published: June 2024

AbstractDelving into fault diagnosis techniques for electrical machines, this comprehensive review focuses on three-phase induction motors. It covers various fault types including eccentricity, broken rotor bars, and bearing faults, discussing techniques such as Motor Current Signature Analysis (MCSA), partial discharge testing, and AI-based approaches. Providing insights into fault detection mechanisms, it emphasizes early identification for optimal machine performance and reliability. With a detailed examination of both traditional and advanced methods, the review serves as a valuable resource for practitioners and researchers in the field, facilitating informed decision-making for maintenance strategies and enhancing machine efficiency.
Optimizing substrate moisture content for enhanced larval survival and growth performance in Hermetia illucens: exploring novel approaches
Authors: Frooninckx L. , Broeckx L. , Goossens S. , Wuyts A. , Van Miert S. 
Published: June 2024

AbstractInsects are receiving a lot of attention as an alternative protein source, especially the black soldier fly, Hermetia illucens. This insect is of particular interest due to its potential as a waste converter as it can be grown on low-value organic substrates, such as agricultural side-streams, manure and decomposing materials. It’s capacity to turn low-value biomass into high-quality protein provide new opportunities in organic waste management. Unfortunately, rearing of black soldier fly larvae (BSFL) does not run efficiently on most substrates. Several parameters influence the rearing efficiency of BSFL. One of the parameters that is often overlooked is the moisture content of the substrate and the physical properties, such as water release/holding, associated with it. In this study the water release properties of 3 BSFL feeds, each at a dry matter content ranging from 10 to 90%, were investigated using 4 different methods, being water release by (1) filtration, (2) sedimentation, (3) syneresis and (4) centrifugation. Rearing of BSFL on the substrates with different dry matter content showed that water release influences larval performance and survival. Moreover, correlating survival to water release indicated that when substrates are hydrated to the minimum dry matter content, at which no water release is measured by the sedimentation method, the highest BSFL survival can be achieved for that substrate. In addition, a standardised method for determining the most optimal dry matter content of substrates based on substrate water holding capacity was developed.