bluuebunny/crossref_metadata_2025_split huggingface dataset:
Color stability of precolored and extrinsically colored monolithic multilayered polychromatic zirconia: Effects of surface finishing and aging
Authors: Abounassif Feras M. , Alfaraj Amal , Gadah Thuraya , Yang Chao‐Chieh , Chu Tien‐Min G. , Lin Wei‐Shao 
Published: June 2024

AbstractPurposeTo explore the impact of zirconia types, coloring methods, and surface finishing on the color stability of monolithic multilayered polychromatic zirconia after artificial aging, including thermocycling and simulated toothbrushing.Materials and MethodsEighty square‐shaped zirconia samples were divided into 2 types (M3Y‐TZP and M6Y‐PSZ), further categorized based on coloring methods (precolored and extrinsically colored) and surface finishing techniques (mechanical polishing or glazing). The color stability was assessed using the CIEDE2000 formula. Artificial aging was simulated via thermocycling and toothbrushing. All samples were analyzed with a spectrophotometer to determine the post‐aging color changes (ΔE00). The ΔE00 were interpreted and classified using the 50:50% perceptibility threshold (PT) and the 50:50% acceptability threshold (AT). Comparisons between groups for ΔE00 differences were performed using three‐way ANOVA, with pairwise comparisons facilitated by Fisher's protected least significant difference test, α = 0.05.ResultsThe study results indicated significant impacts of zirconia type, coloring method, and surface finishing on color stability. The M6Y groups experienced significantly greater color changes (6.61 ± 1.63) compared to the M3Y groups (3.40 ± 2.24), p < 0.0001. For both types of zirconia, extrinsically colored samples exhibited significantly higher ΔE00 when mechanically polished (p = 0.004). However, surface finishing had no significant effect on ΔE00 in precolored samples of either zirconia material (p = 1.000). The evaluation and categorization of ΔE00 variations indicated that nearly all color changes in the M6Y groups, regardless of being precolored, extrinsically colored, polished, or glazed, were deemed extremely unacceptable (Grade 1). In contrast, the M3Y groups showed more acceptable results, with the majority of color changes classified as moderately unacceptable (Grade 3).ConclusionsThe color stability of multilayered polychromatic zirconia is influenced by the type of material, extrinsic coloring, and the chosen surface treatment post‐artificial aging. The translucent 6Y‐PSZ exhibited lower color stability, especially with only mechanical polishing. For the fabrication of M3Y‐TZP and 6Y‐PSZ monolithic multilayered polychromatic zirconia restorations, extrinsic coloring should be paired with glazing to maintain color stability. Conversely, in the absence of extrinsic coloring, both glazing and mechanical polishing are effective in preserving color stability.
Protein supplementation during mid‐gestation affects maternal voluntary feed intake, performance, digestibility, and uterine blood flow of beef cows
Authors: Meneses Javier A. M. , Nascimento Karolina B. , Galvão Matheus Castilho , Moreira Gabriel M. , Chalfun Luthesco Haddad Lima , Souza Stefania Priscilla de , Ramírez‐Zamudio German D. , Ladeira Marcio Machado , Duarte Marcio S. , Casagrande Daniel R. , Gionbelli Mateus P. 
Published: June 2024

AbstractThis study aimed to assess the impact of protein supplementation and its interaction with calf sex (CS) on the performance, metabolism and physiology of pregnant beef cows. Fifty‐two multiparous Zebu beef cows carrying female (n = 22) and male (n = 30) fetuses were used. Cows were individually housed from day 100 to 200 of gestation and randomly assigned to restricted (RES, n = 26) or supplemented (SUP, n = 26) groups. The RES cows were ad libitum fed a basal diet (corn silage + sugarcane bagasse + mineral mixture), achieving 5.5% crude protein (CP), while SUP cows received the same basal diet plus a protein supplement (40% CP, at 3.5 g/kg of body weight). All cows were fed the same diet during late gestation. Differences were declared at p < 0.05. No significant interaction between maternal nutrition and calf sex was found for maternal outcomes (p ≥ 0.34). The SUP treatment increased the total dry matter (DM) intake (p ≤ 0.01) by 32% and 19% at mid‐ and late‐gestation respectively. The total tract digestibility of all diet components was improved by SUP treatment at day 200 of gestation (p ≤ 0.02), as well as the ruminal microbial CP production (p ≤ 0.01). The SUP treatment increased (p ≤ 0.03) the cows' body score condition, ribeye area, the average daily gain (ADG) of pregnant components (PREG; i.e., weight accretion of cows caused by pregnancy) and the ADG of maternal tissues (i.e., weight accretion discounting the gain related to gestation) in the mid‐gestation. The SUP cows exhibited a lower maternal ADG (p < 0.01) compared to RES cows in late pregnancy. There was a 24% additional gain (p < 0.01) in the PREG components for SUP cows during late gestation, which in turn improved the calf birthweight (p = 0.05). The uterine arterial resistance and pulsatility indexes (p ≤ 0.01) at mid‐gestation were greater for RES cows. In conclusion, protein supplementation during mid‐gestation is an effective practice for improving maternal performance, growth of the gravid uterus and the offspring's birth weight.
Impact of COVID‐19 pandemic on hepatocellular carcinoma surveillance in British Columbia, Canada: An interrupted time series study
Authors: Makuza Jean Damascene , Wong Stanley , Morrow Richard L. , Binka Mawuena , Darvishian Maryam , Jeong Dahn , Adu Prince A. , Cua Georgine , Yu Amanda , Velásquez García Hector A. , Bartlett Sofia R. , Yoshida Eric , Ramji Alnoor , Krajden Mel , Janjua Naveed Z. 
Published: June 2024

AbstractWe assessed the impact of the COVID‐19 pandemic on hepatocellular carcinoma (HCC) surveillance among individuals with HCV diagnosed with cirrhosis in British Columbia (BC), Canada. We used data from the British Columbia Hepatitis Testers Cohort (BC‐HTC), including all individuals in the province tested for or diagnosed with HCV from 1 January 1990 to 31 December 2015, to assess HCC surveillance. To analyse the impact of the pandemic on HCC surveillance, we used pre‐policy (January 2018 to February 2020) and post‐policy (March to December 2020) periods. We conducted interrupted time series (ITS) analysis using a segmented linear regression model and included first‐order autocorrelation terms. From January 2018 to December 2020, 6546 HCC screenings were performed among 3429 individuals with HCV and cirrhosis. The ITS model showed an immediate decrease in HCC screenings in March and April 2020, with an overall level change of −71 screenings [95% confidence interval (CI): −105.9, −18.9]. We observed a significant decrease in HCC surveillance among study participants, regardless of HCV treatment status and age group, with the sharpest decrease among untreated HCV patients. A recovery of HCC surveillance followed this decline, reflected in an increasing trend of 7.8 screenings (95% CI: 0.6, 13.5) per month during the post‐policy period. There was no level or trend change in the number of individuals diagnosed with HCC. We observed a sharp decline in HCC surveillance among people living with HCV and cirrhosis in BC following the COVID‐19 pandemic control measures. HCC screening returned to pre‐pandemic levels by mid‐2020.
Effects of exposure to multiple metallic elements in the first trimester of pregnancy on the risk of preterm birth
Authors: Wu Ting , Luo Chuan , Li Tao , Zhang Chen , Chen Hui‐Xi , Mao Yi‐Ting , Wu Yan‐Ting , Huang He‐Feng 
Published: June 2024

AbstractExposure to certain heavy metals has been demonstrated to be associated with a higher risk of preterm birth (PTB). However, studies focused on the effects of other metal mixtures were limited. A nested case‒control study enrolling 94 PTB cases and 282 controls was conducted. Metallic elements were detected in maternal plasma collected in the first trimester using inductively coupled plasma‒mass spectrometry. The effect of maternal exposure on the risk of PTB was investigated using logistic regression, least absolute shrinkage and selection operator, restricted cubic spline (RCS), quantile g computation (QGC) and Bayesian kernel machine regression (BKMR). Vanadium (V) and arsenic (As) were positively associated with PTB risk in the logistic model, and V remains positively associated in the multi‐exposure logistic model. QGC analysis determined V (69.42%) and nickel (Ni) (70.30%) as the maximum positive and negative contributors to the PTB risk, respectively. BKMR models further demonstrated a positive relationship between the exposure levels of the mixtures and PTB risk, and V was identified as the most important independent variable among the elements. RCS analysis showed an inverted U‐shape effect of V and gestational age, and plasma V more than 2.18 μg/L was considered a risk factor for shortened gestation length. Exposure to metallic elements mixtures consisting of V, As, cobalt, Ni, chromium and manganese in the first trimester was associated with an increased risk of PTB, and V was considered the most important factor in the mixtures in promoting the incidence of PTB.
A rendering‐based lightweight network for segmentation of high‐resolution crack images
Authors: Chu Honghu , Yu Diran , Chen Weiwei , Ma Jun , Deng Lu 
Published: June 2024

AbstractHigh‐resolution (HR) crack images provide detailed structural assessments crucial for maintenance planning. However, the discrete nature of feature extraction in mainstream deep learning algorithms and computational limitations hinder refined segmentation. This study introduces a rendering‐based lightweight crack segmentation network (RLCSN) designed to efficiently predict refined masks for HR crack images. The RLCSN combines a deep semantic feature extraction architecture—merging Transformer with a super‐resolution boundary‐guided branch—to reduce environmental noise and preserve crack edge details. It also incorporates customized point‐wise refined rendering for training and inference, focusing computational resources on critical areas, and an efficient sparse training method to ensure efficient inference on commercial mobile computing platforms. Each RLCSN's components are validated through ablation studies and field tests, demonstrating its capability to enable unmanned aerial vehicle‐based inspections to detect cracks as narrow as 0.15 mm from a distance of 3 m, thereby enhancing inspection safety and efficiency.
Evidence of microbiota–host dysbiosis between periodontitis and cerebral small vessel disease
Authors: Zhuang Jiabao , Zhang Shufan , Chen Huiwen , Qiu Che , Feng Tienan , Zhou Wei , Han Xiang , Song Zhongchen 
Published: June 2024

AbstractObjectivesTo investigate the correlation between periodontitis and cerebral small vessel disease (CSVD) from the clinical and microbiological aspects.Subjects and methodsPeriodontitis patients (CP group, n = 31) and CSVD patients (CSVD group, n = 30) were examined for neurological and periodontal condition. Subgingival plaque was collected and performed using 16S rRNA sequencing. Logistic regression and LASSO regression were used to analyze the periodontal parameters and subgingival microbiota related to CSVD, respectively. Inflammatory factors in gingival crevicular fluid (GCF) were also detected and compared between the two groups.ResultsClinical attachment level (CAL), teeth number and plaque index demonstrated a significant difference between CP and CSVD group, meanwhile, CAL was independently associated with CSVD. Besides, the microbial richness and composition were distinct between two groups. Five genera related to periodontal pathogens (Treponema, Prevotella, Streptococcus, Fusobacterium, Porphyromonas) were screened out by LASSO regression, suggesting a potential association with CSVD. Finally, the levels of inflammatory factors in GCF were statistically higher in CSVD group than those in CP group.ConclusionsCerebral small vessel disease patients demonstrated worse periodontal condition, meanwhile the interaction between microbiota dysbiosis and host factors (inflammation) leading to a better understanding of the association between periodontitis and CSVD.
The superior allele LEA12OR in wild rice enhances salt tolerance and yield
Authors: Ge Yuwei , Chen Gaoming , Cheng Xinran , Li Chao , Tian Yunlu , Chi Wenchao , Li Jin , Dai Zhaoyang , Wang Chunyuan , Duan Erchao , Liu Yan , Sun Zhiguang , Li Jingfang , Wang Baoxiang , Xu Dayong , Sun Xianjun , Zhang Hui , Zhang Wenhua , Wang Chunming , Wan Jianmin 
Published: June 2024

SummarySoil salinity has negative impacts on food security and sustainable agriculture. Ion homeostasis, osmotic adjustment and reactive oxygen species scavenging are the main approaches utilized by rice to resist salt stress. Breeding rice cultivars with high salt tolerance (ST) and yield is a significant challenge due to the lack of elite alleles conferring ST. Here, we report that the elite allele LEA12OR, which encodes a late embryogenesis abundant (LEA) protein from the wild rice Oryza rufipogon Griff., improves osmotic adjustment and increases yield under salt stress. Mechanistically, LEA12OR, as the early regulator of the LEA12OR‐OsSAPK10‐OsbZIP86‐OsNCED3 functional module, maintains the kinase stability of OsSAPK10 under salt stress, thereby conferring ST by promoting abscisic acid biosynthesis and accumulation in rice. The superior allele LEA12OR provides a new avenue for improving ST and yield via the application of LEA12OR in current rice through molecular breeding and genome editing.
Isolation and evaluation of novel male sterile and self‐incompatible mutant lines of mungbean (Vigna radiata L.)
Authors: Rahevar P. M. , Chauhan R. M. , Patel P. T. , Patel M. P. , Bhadauria H. S. , Solanki S. D. , Viradiya Y. A. , Gami R. A. , Vaghela S. J. 
Published: June 2024

AbstractHeterosis has long been harnessed in crop production, and while crop fertility lays the groundwork for leveraging heterosis, there remains a dearth of comprehensive data regarding genic male sterility in mungbean. Mungbean (Vigna radiata (L.) Wilczek), being a self‐pollinating crop, exhibits significant hybrid vigour in F1 hybrid seed yield, suggesting the potential for developing hybrid varieties to propel mungbean yield beyond existing plateaus. A new male sterile mutant was induced, isolated, stabilized and evaluated, via gamma irradiation at a rate of 600 Gy, identified in the M3 generation of the GM 4 accession. A uniform male sterile line was crossbred with the wild‐type parental plant to explore the inheritance pattern of male sterility. Pollen sterility was validated through the acetocarmine test, while stigma viability was ascertained using the hydrogen peroxide. While all F1 plants displayed fertile flowers, the F2 generation showed a clear 3:1 segregation ratio for fertile to male sterile plants, indicating the control of male sterility by a single recessive gene, mms. Also, a novel existence of a truncated stigma nestled within the anther column inhibits the effective reception of pollen during anthesis, offering potential in reducing emasculation time by a slight cut on the flower bud and enhancing cross pollination during hybrid seed production. The isolation of these two mutants is poised to significantly advance the global mungbean hybrid breeding programme.
Multivariate sharp‐wave ripples in schizophrenia during awake state
Authors: Ohki Takefumi , Chao Zenas C. , Takei Yuichi , Kato Yutaka , Sunaga Masakazu , Suto Tomohiro , Tagawa Minami , Fukuda Masato 
Published: June 2024

AimsSchizophrenia (SZ) is a brain disorder characterized by psychotic symptoms and cognitive dysfunction. Recently, irregularities in sharp‐wave ripples (SPW‐Rs) have been reported in SZ. As SPW‐Rs play a critical role in memory, their irregularities can cause psychotic symptoms and cognitive dysfunction in patients with SZ. In this study, we investigated the SPW‐Rs in human SZ.MethodsWe measured whole‐brain activity using magnetoencephalography (MEG) in patients with SZ (n = 20) and sex‐ and age‐matched healthy participants (n = 20) during open‐eye rest. We identified SPW‐Rs and analyzed their occurrence and time‐frequency traits. Furthermore, we developed a novel multivariate analysis method, termed “ripple‐gedMEG” to extract the global features of SPW‐Rs. We also examined the association between SPW‐Rs and brain state transitions. The outcomes of these analyses were modeled to predict the positive and negative syndrome scale (PANSS) scores of SZ.ResultsWe found that SPW‐Rs in the SZ (1) occurred more frequently, (2) the delay of the coupling phase (3) appeared in different brain areas, (4) consisted of a less organized spatiotemporal pattern, and (5) were less involved in brain state transitions. Finally, some of the neural features associated with the SPW‐Rs were found to be PANSS‐positive, a pathological indicator of SZ. These results suggest that widespread but disorganized SPW‐Rs underlies the symptoms of SZ.ConclusionWe identified irregularities in SPW‐Rs in SZ and confirmed that their alternations were strongly associated with SZ neuropathology. These results suggest a new direction for human SZ research.
What Italian PICU Healthcare Providers Think About Donation After Cardiocirculatory Determination of Death: A National Survey
Authors: Giugni Cristina , Cecchi Costanza , Giometto Sabrina , Lucenteforte Ersilia , Mondardini Cristina , Chiusolo Fabrizio , Giannini Alberto , Astuto Marinella , Figini Maria , Piazza Marcello , Tosoni Alvise , Ricci Zaccaria 
Published: June 2024

ABSTRACTBackgroundItaly presently does not have a pediatric organ donation program after cardiocirculatory determination of death (pDCDD). Before implementing a pDCDD program, many centers globally have conducted studies on the attitudes of pediatric intensive care unit (PICU) staff. This research aims to minimize potential adverse reactions and evaluate the acceptance of the novel donation practice.MethodsWe conducted an electronic and anonymous survey on attitudes toward pDCDD among healthcare professionals (HCPs) working at eight Italian PICUs. The survey had three parts: (I) questions about general demographic data; (II) 18 statements about personal wishes to donate, experience of discussing donation, and knowledge about donation; (III) attitudinal statements regarding two pediatric Maastricht III scenarios of organ donation.ResultsThe response rate was 54.4%, and the majority of respondents were nurses. Of those who responded, 45.3% worked in the Center, 40.8% in the North, and 12.8% in the South of Italy. In total, 93.9% supported pediatric organ and tissue donation, 90.3% supported donation after neurological determination of death (DNDD), 78.2% supported pDCDD, and 69.7% felt comfortable about the idea of participating in pDCDD on Type III patients, with a higher percentage of supportive responses in the Center (77.2%) than in the North (65.1%) and South (54.5%) of Italy (p‐value < 0.004). Concerning scenarios, 79.3% of participants believed that organ retrieval took place in a patient who was already deceased. Overall, 27.3% considered their knowledge about DCDD to be adequate.ConclusionsOur study provides insight into the attitudes and knowledge of PICU staff members regarding pDCDD in Italy. Despite a general lack of knowledge on the subject, respondents showed positive attitudes toward pDCDD and a strong consensus that the Italian legislation protocol for determining death based on cardiocirculatory criteria respects the “dead donor rule.” There were several distinctions among the northern, central, and southern regions of Italy, and in our view, these disparities can be attributed to the varying practices of commemorating the deceased. In order to assess how practice and training influence the attitude of PICU staff members, it would be interesting to repeat the survey after the implementation of a program.
Addressing Substance Use in the Adolescent Transplant Population
Authors: Williams Brian S. , Kaye Jesse T. 
Published: June 2024

ABSTRACTAdolescence is a unique period of physical and psychosocial changes as youth transition, over many years, to adulthood. The psychosocial changes that accompany adolescence include emotional separation from parents, greater influence of peer groups, an interest in self‐identification and autonomy, and increased risk‐taking behaviors. Substance use is a common form of risk‐taking behavior in the adolescent developmental stage. Alcohol, nicotine, and cannabis are the most common types of substances used in the United States. In the adolescent transplant population, rates of substance use appear to be at, or slightly below, their peer counterparts. Substance use can lead to deleterious health outcomes for adolescent transplant patients as a result of impaired decision‐making, reduction in medication and clinic visit compliance, increases in mental health disorders, and risk for developing dependence and a substance use disorder. Given the close relationship that many pediatric transplant providers have with their patients and families, transplant care teams are in an excellent position to help their patients by addressing adolescent substance use. This narrative review describes how providers can use proactive standardized approaches to identify and intervene with substance use behavior.
YABBY transcription factor family in the octoploid Fragaria × ananassa and five diploid Fragaria species
Authors: Jara‐Cornejo K. , Zúñiga P. E. , Rivera‐Mora C. , Bustos E. , Garrido‐Bigotes A. , Ruiz‐Lara S. , Figueroa C. R. 
Published: June 2024

Abstract

YABBY genes encode specific TFs of seed plants involved in development and formation of leaves, flowers, and fruit. In the present work, genome‐wide and expression analyses of the YABBY gene family were performed in six species of the Fragaria genus: Fragaria × ananassa, F. daltoniana, F. nilgerrensis, F. pentaphylla, F. viridis, and F. vesca.

The chromosomal location, synteny pattern, gene structure, and phylogenetic analyses were carried out. By combining RNA‐seq data and RT‐qPCR analysis we explored specific expression of YABBYs in F. × ananassa and F. vesca. We also analysed the promoter regions of FaYABBYs and performed MeJA application to F. × ananassa fruit to observe effects on gene expression.

We identified and characterized 25 YABBY genes in F. × ananassa and six in each of the other five species, which belong to FIL/YAB3 (YABBY1), YAB2 (YABBY2), YAB5 (YABBY5), CRC, and INO clades previously described. Division of the YABBY1 clade into YABBY1.1 and YABBY1.2 subclades is reported. We observed differential expression according to tissue, where some FaYABBYs are expressed mainly in leaves and flowers and to a minor extent during fruit development of F. × ananassa. Specifically, the FaINO genes contain jasmonate‐responsive cis‐acting elements in their promoters which may be functional since FaINOs are upregulated in F. × ananassa fruit under MeJA treatment.

This study suggests that YABBY TFs play an important role in the development‐ and environment‐associated responses of the Fragaria genus.