bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNAi‐mediated silencing of ezrin gene reverses malignant behavior of human gastric cancer cell line SGC‐7901
Authors: WANG Hong Jian , ZHU Jin Shui , ZHANG Qiang , GUO Hua , DAI Yun Hai , XIONG Xiao Peng 
Published: October 2009

OBJECTIVE:  To investigate the effects of ezrin targeting gene of RNA interference (RNAi) on human gastric cancer cell line SGC‐7901 in vitro.METHODS:  The highly metastatic human gastric cancer cell line SGC‐7901 transfected with a small interfering (siRNA) lentivirus vector was selected for this research study. Expressions of ezrin mRNA and ezrin protein in the SGC‐7901 cells were detected using RT‐PCR and Western blot. Cell apoptosis was observed using flow cytometry. Transwell invasion and the cell adhesion test were used to verify the effect of RNAi on ezrin expression in the human gastric cancer cell line SGC‐7901 in vitro.RESULTS:  Ezrin gene targeting via a RNAi‐mediated lentivirus vector had obvious inhibitory effects on ezrin expression in the human gastric cancer cell line SGC‐7901. The results of the RT‐PCR show the obvious inhibition of ezrin mRNA expression in Eai and Ebi groups (0.22 ± 0.01 vs 0.95 ± 0.04, P < 0.05; 0.31 ± 0.01 vs. 0.95 ± 0.04, P < 0.05). Western blot analysis revealed a 72.35 ± 3.74% reduction of the ezrin protein level after interference with the ezrin targeting gene. Moreover, the inhibition of ezrin expression clearly inhibited SGC‐7901 cell migration and invasion, and improved cell adhesion as well as increased sensitivity to camptothecin‐induced apoptosis.CONCLUSION:  Ezrin gene targeting by RNAi can inhibit the metastatic growth and migration of SGC‐7901 human gastric cancer cells.
Association of the human leukocyte antigen class II alleles with chronic atrophic gastritis and gastric carcinoma in Koreans
Authors: LEE Hae‐Wan , HAHM Ki‐Baik , LEE Jeong Sang , JU Young‐Su , LEE Kee Myung , LEE Kyung Wha 
Published: October 2009

OBJECTIVE:  Gastric carcinogenesis is a multi‐step process and is influenced by several etiological agents, including the host's genetic factors. Since whether a patient remains with chronic superficial gastritis (CSG) or progresses to either chronic atrophic gastritis (CAG) or gastric carcinoma (GC) could be a genetic predisposition unique in each population, we hypothesized that host human leukocyte antigen (HLA) alleles could be discriminative in predicting the risk of CSG progression to precancerous CAG and GC in Koreans.METHODS:  A total of 165 patients with gastric disorders (CSG, 62; CAG, 69 and GC, 34), were selected to investigate the association of HLA class II alleles with the progression of CSG to CAG or GC. HLA genotypes were obtained by the polymerase chain reaction‐sequence based typing method.RESULTS:  The phenotypic frequencies of DRB1*1101 and DQA1*0505 were significantly higher in the CAG group compared to those in the CSG group. In the subjects with Helicobacter pylori (H. pypori) (+), the frequencies of DRB1*1501 and DQB1*0602 were significantly lower in the CAG compared to those in the CSG. Further analysis showed that sex (P < 0.05, OR= 0.41–0.42) and age (P < 0.05, OR= 1.05) also affected the risk of progression from CSG to CAG in H. pylori (+) patients carrying the DRB1*1501 or DQB1*0602 allele. Additionally, the frequency of DRB1*0404 in the GC group was significantly higher than that in the gastritis group.CONCLUSION:  Our findings strongly imply an association between HLA class II alleles and the risk of CAG development and GC progression in Koreans.
Interventional effect of Ginkgo biloba extract on the progression of gastric precancerous lesions in rats
Authors: JIANG Xiao Yun , QIAN Li Ping , ZHENG Xiao Juan , XIA Yu Ye , JIANG Yi Bin , SUN Da Yu 
Published: October 2009

OBJECTIVE:  To investigate the effect of Ginkgo biloba extract on gastric precancerous lesions in rats.METHODS:  80 4‐week‐old Wistar rats were randomly divided into four groups: a control group, a model group, a low and a high dose Ginkgo biloba extract intervention group; 20 in each group. Gastric precancerous lesions were induced by giving them 100 mg/L N‐methyl‐N′‐nitro‐N‐nitrosoguanidine (MNNG) solution to drink ad libitum for 20 weeks. In addition to the MNNG, the intervention groups were lavaged with Ginkgo biloba extract (0.5 mg/kg/d in the low dose group, 1.5 mg/kg/d in the high dose group) for 20 weeks. Starting from week 21 all the rats were fed with normal rat chow and tap water. At the end of week 30 the rats were killed. The histopathological changes of their gastric mucosa, ISA, NGI, the serum and gastric mucosal SOD/MDA and the expressions of oncogenes were studied.RESULTS:  The incidence of mild to severe intestinal metaplasia and dysplasia were significantly lower in the intervention groups than those in the model group (P < 0.01). The ISA and NGI in the intervention groups were significantly lower than those in the model group (P < 0.01). In the intervention groups the activity of SOD was increased and the concentration of MDA was decreased (P < 0.01). Expressions of Bcl‐2, c‐myc and FasL decreased in the intevention groups, whereas the expression of Fas increased. When compared with the model group, the differences were statistically significant (P < 0.01, P < 0.05, respectively).CONCLUSION:  Ginkgo biloba extract can increase anti‐oxidative activity and inhibit the progression of gastric precancerous lesions via the regulation of cell proliferation and apoptosis.
Direct international normalized ratio determination using multicalibrators is more responsive than the conventional method for measuring prothrombin time
Authors: CHA C.‐H. , PARK C.‐J. , KIM D. H. , KIM M.‐J. , CHO Y.‐U. , JANG S. , CHI H.‐S. 
Published: October 2009

SummaryDirect international normalized ratio (INR) determination using certified INR plasmas was shown to improve precision and accuracy. We evaluated the utility of a multicalibrator in determining INR. INR values were measured in 493 blood samples from patients subjected to anticoagulation therapy (320) and control subjects (173). Study was performed using CA‐7000 coagulation analyzer (Sysmex, Japan) with Thromborel S (Dade Behring, Germany). Direct INR values were obtained using PT‐Multi Calibrator (Dade Behring) composed of five lyophilized calibrant plasmas. Conventional INR values were calculated from mean normal prothrombin time and instrument/reagent‐specific international sensitivity index (ISI). We compared the difference between the INR results obtained with the two methods. The mean INR value of direct INR method was significantly higher than that of conventional method. The differences in values (direct INR – conventional INR) generated using the two methods increased in proportion to the INR values. Elevation of INR was observed in data obtained with the direct INR method, compared with conventional INR values. Accordingly, we conclude that direct INR method is more responsive than conventional method in determining INR.
Correlation between radiculalgia and counts of T lymphocyte subsets in the peripheral blood of patients with lumbar disc herniation
Authors: Tian Peng , Ma Xin‐long , Wang Tao , Ma Jian‐xiong , Yang Xue 
Published: October 2009

Objective:  To determine the correlation between the degree of radiculalgia and counts of T lymphocyte subsets in the peripheral blood of patients with lumbar disc herniation.Methods:  Forty‐nine patients with lumbar disc herniation (group A) were divided into three subgroups according to Visual Analogue Scale (VAS) pain scores (group A1: n= 12, VAS 0–4.0; A2: n= 24, VAS 4.1–7.0; A3: n= 13, VAS 7.1–10.0. Twenty health blood donors who volunteered to be involved in the study comprised the control group (group B). Peripheral blood counts of various T lymphocyte subsets were measured in each group.Results:  (i) The counts of CD4+ T and CD4+/CD8+ lymphocytes were higher in group A than in group B, and the difference between the two groups was statistically significant (P < 0.05). There were also statistically significant differences between group A and group B in the counts of CD3+ and CD8+ T lymphocytes (P < 0.05); (ii) There was no correlation between the VAS scores and the counts of CD3+ T lymphocytes (r= 0.194, P > 0.05). A strong significant correlation was observed between the VAS scores and counts of CD4+ T lymphocytes (r= 0.542, P < 0.05), CD4+/CD8+ (r= 0.468, P < 0.05), which increased with increasing VAS scores in the three subgroups of group A (P < 0.05). However there was a significant negative linear correlation between CD8+ T lymphocyte counts and pain scores (r=−0.462, P < 0.05).Conclusion:  Our results suggest that changes in T lymphocyte subsets in peripheral blood take place after prolapse of lumbar intervertebral discs. The current results may provide support for involvement of immunologic mechanisms in low back pain secondary to herniation of the lumbar disc. T lymphocytes may play an important role in the development of symptoms in patients with lumbar intervertebral disc herniation.
Plasminogen activator inhibitor 1 gene polymorphisms and mirtazapine responses in Koreans with major depression
Authors: Chang Hun Soo , Lee Hwa‐Young , Ham Byung‐Joo , Kang Rhee‐Hun , Jeong Yoo‐Jung , Kim Hyang‐Mi , Hahn Sang‐Woo , Lee Min‐Soo 
Published: October 2009

AbstractIntroduction: Brain‐derived neurotrophic factor (BDNF) is involved in the pathophysiology of mental disorders and in the mechanism of action of antidepressant medications. The mature form of BDNF is derived from proBDNF through tissue type plasminogen activator (tPA) and the plasminogen system in the brain, which is regulated by an endogenous inhibitor, plasminogen activator inhibitor (PAI). Therefore, PAI may be involved in the development of major depressive disorder (MDD) and its response to antidepressant treatment. The present study determined the relationship between the 4G/5G polymorphism in the PAI1 gene and the clinical outcome of mirtazapine treatment in 271 Korean MDD patients.Methods: We tested the association between the polymorphism and response to mirtazapine treatment or percentage decrease of the 21‐item Hamilton Depression Rating (HAMD21) scores using multiple logistic and linear regression analysis.Results: PAI1 4G/5G genotypes and allele distributions were comparable between responders and non‐responders during the treatment period. Similarly, linear regression showed no association between genotypes or alleles and the percentage decline in total HAMD21 with mirtazapine treatment. In the analysis of symptomatic subscores, the percentage decline in the psychic anxiety and delusion scores after 4 weeks of mirtazapine treatment showed a statistical trend to a difference among genotypes, although it was not statistically significance.Discussion: In this first pharmacogenetics study of the PAI1 4G/5G polymorphism and mirtazapine treatment response, our results do not support the hypothesis that this polymorphism is involved in the therapeutic response to mirtazapine.
Laparoscopic anterior resection for rectosigmoid cancer: Patient outcomes after implementation of a clinical pathway
Authors: Hsu T.W. , Chiang F.F. , Wang H.M. 
Published: October 2009

AbstractIntroduction: A clinical pathway designed for a single type of laparoscopic colorectal surgery for cancer might be helpful in decreasing complication rates and total hospital costs. It has been reported to be effective in reducing costs and shortening length of hospital stays in many situations such as laparoscopic cholecystectomy, colon resection, total colectomy, and gastrointestinal bleeding, as well as when caring for patients in the intensive care unit.Materials and Methods: A clinical pathway, including surgical details and perioperative management, for patients undergoing laparoscopic anterior resection for rectosigmoid cancer was designed and implemented. From January 2003 to December 2006, it was applied to 80 patients.Results: The average length of a hospital stay for these patients was 9.06 d. The mean hospital stay and total cost decreased year by year. The overall complication rate was 8.75% without perioperative mortality, and 47.5% of patients with underlying diseases were treated safely.Discussion: Laparoscopic anterior resection for rectosigmoid cancer, with curative or palliative intent, was safe after standardization of surgical details and perioperative management. The total hospital costs for each patient was predictable and decreased year by year.
Making Science of Influencing: Assessing the Impact of Development Research
Authors: Sumner Andy , Ishmael‐Perkins Nick , Lindstrom Johanna 
Published: October 2009

SummaryThe impact and influence of development research is an agenda that has been gathering momentum over the last few years. This agenda is a coming together of two divergent concerns. The first, from the funders of research, draws on results based management and is concerned with getting value‐for‐money from research spending or with ‘more bang for the buck’. The second, more typical of those in the development studies research community, is concerned with whether research in the area is ‘making a difference’. Among development researchers there is also often a political or normative basis – addressing global poverty and inequality – and catalysing change.The meanings of the terms' impact and influence are multiple, multi‐layered and complex to track. They may refer to use (i.e. consideration) or actual outcome(s) of social change. They can be visible or invisible; progressive or regressive. Impacts and influence can be intended or unintended and immediate or long‐term. The processes of impact and influence are acknowledged to be non‐linear, iterative and complex.This paper considers the impact and influence of development research from a plurality of perspectives. Interest in the impact/influence of research projects aggregates upwards to support the overall case for (often public) funding of development research (in areas that are likely to play a major role in the next few years in changing the climate for public expenditure in light of the global financial crisis).
Aquaculture impacts on the algal and bacterial communities in a small boreal forest lakeThis paper is part of the series “Forty Years of Aquatic Research at the Experimental Lakes Area”.
Authors: Findlay David L. , Podemski Cheryl L. , Kasian Susan E.M. 
Published: October 2009

A whole-lake experiment to examine the impacts of aquaculture on a freshwater ecosystem was conducted at the Experimental Lakes Area in northwestern Ontario, Canada. From 2003 to 2006, a 10 tonne fish capacity aquaculture cage stocked with rainbow trout ( Oncorhynchus mykiss ) was operated in Lake 375 and the impact of excess nutrients on the algal and bacteria communities was examined. The experiment was designed as a nutrient loading experiment with fish food and fish excretion the source of nutrients. Total N and P concentrations increased over the 4 years (15× and 4×, respectively). Phytoplankton biomass increased 4× annually following the start of aquaculture operation in 2003. The most dramatic responses occurred during spring and fall mixing, with blooms of chrysophytes and dinoflagellates increasing biomass by up to 12×. Bacteria biomass and densities were unaffected except for increases in late fall. Periphyton biomass was relatively unaffected except for an increase in biomass in the fourth year. The combination of a long water residence time in the lake coupled with an extremely high fish stocking density in Lake 375 resulted in an immediate impact on water quality. The results suggest that the impacts of aquaculture are accumulative and continual stocking will further impact water quality.
Performance of temperature and dissolved oxygen criteria to predict habitat use by lake trout (Salvelinus namaycush)This paper is part of the series “Forty Years of Aquatic Research at the Experimental Lakes Area”.
Authors: Plumb John M. , Blanchfield Paul J. 
Published: October 2009

We compared theoretical habitat volumes, determined from traditional combinations of temperature and dissolved oxygen concentration (DO) boundaries, with in situ habitat use by acoustically tagged lake trout ( Salvelinus namaycush ). The widely used criteria of 8–12 °C underestimated lake trout habitat use by 68%–80%. Instead, combined temperature (<12 or 15 °C) and DO (>4 or 6 mg·L–1) criteria most closely matched lake trout habitat use, had a similar seasonal trend as the tagged fish, suggested modest reductions (5% of total lake volume) in habitat during a warmer year, and performed best when the constraints of temperature and DO were most limiting. All data were collected in a small boreal shield lake (27 ha, zmax = 21 m) at the Experimental Lakes Area in northwestern Ontario, Canada, during two contrasting periods of thermal stratification (2003: warmer and longer; 2004: cooler and shorter), providing an assessment of observed and theoretical habitat volumes over current environmental extremes.
QSPR studies on normal boiling points and molar refractivities of organic compounds by correlation-ranking-based PCR and PC–ANN analyses of new topological indices
Authors: Ghavami Raouf , Najafi Amir , Hemmateenejad Bahram 
Published: October 2009

The new topological indices (Sh indices) based on the distance sum and connectivity of a molecular graph, previously developed by our team, were extended to predict the two physicochemical properties, including normal boiling point (NBP) and molar refractivity (MR), of a large set of organic compounds consisting of alkanes, alkenes, ethers, amines, alcohols, alkylbenzenes, and alkylhalides. The sets of molecular descriptors were derived directly from the two-dimensional molecular structure of the compounds based on graph theory. Both linear and nonlinear modelings were implemented by using principal component regression (PCR) and principal component – artificial neural network (PC–ANN) with back-propagation learning algorithm, respectively. Eigenvalue and correlation-ranking procedures were used to rank the principal components and entered them into the models. Principal component analysis of Sh data matrix showed that the respective six and seven PCs could explain 97.49% and 99.22% of variances in the Sh indices. PCR analysis of the NBP and MR data demonstrated that the proposed Sh indices could explain about 97.52% and 99.52% of variations, while the variations explained by the PC–ANN modeling were more than 99.00% and 99.82%, respectively. The predictive ability of the models were evaluated using an external test set for NBP and MR of the molecules with the respective root-mean-square errors lower than 9.69 K and 0.660 cm3mol–1for the linear model and 6.17 K and 0.416 cm3mol–1for the nonlinear model.
Cowaxanthone F, a new tetraoxygenated xanthone, and other anti-inflammatory and antioxidant compounds from Garcinia cowa
Authors: Panthong Kanda , Hutadilok-Towatana Nongporn , Panthong Ampai 
Published: October 2009

A new tetraoxygenated xanthone, cowaxanthone F (1), as well as four known compounds, morelloflavone (2), volkensiflavone (3), morelloflavone-7″-O-glucoside (fukugiside, 4), and 1,6-dihydroxyxanthone (5), were isolated from the crude acetone extract of the twigs of Garcinia cowa (Guttiferae). All compounds (1–5) were tested for antioxidant activity against DPPH (diphenylpicrylhydrazyl), hydroxyl, and superoxide radicals; only morelloflavone (2) and morelloflavone-7″-O-glucoside (4) exhibited high potency. Eight tetraoxygenated xanthones from the fruits of G. cowa , cowaxanthones A–D (6–9), cowanin (15), α-mangostin (16), mangostanin (17), and cowanol (18), were also investigated for anti-inflammatory activity using ethyl phenylpropiolate (EPP)-induced ear edema. Assessment at 30, 60, and 120 min revealed that cowaxanthones B–D (7–9), cowanin (15), and α-mangostin (16) exhibited significant anti-inflammatory activity when compared to phenylbutazone, while cowaxanthone A (6), mangostanin (17), and cowanol (18) showed less activity.
Structural control of Au and Au–Pd nanoparticles by selecting capping ligands with varied electronic and steric effects
Authors: Murphy Patrick B. , Liu Feng , Cook Stephen C. , Jahan Nusrat , Marangoni D. Gerrard , Grindley T. Bruce , Zhang Peng 
Published: October 2009

Weakly interacting ligands including three Gemini surfactants, didodecyldimethylammonium bromide (DDAB), and amines (RNH2, R2NH, and R3N) were used to prepare Au nanoparticles (NPs). Aqueous Au NPs capped with DDAB and Gemini surfactants showed similar sizes (3–4 nm), whereas toluene-based NPs stabilized with DDAB, amines, and their mixtures range from 2.5 to 9.3 nm. Ligand effect on Au–Pd NP structure was also studied with EXAFS. These findings were consistently accounted for by considering the ligand’s electronic/steric effects and mixed ligands coadsorption, and suggest useful ways to control NP structure by manipulating the two effects and using mixed capping ligands.
Water-promoted regioselective hydrothiolation of alkynes
Authors: Bhadra Sukalyan , Ranu Brindaban C. 
Published: October 2009

Water promotes hydrothiolation of unactivated alkynes efficiently without any catalyst or additive. The reaction at room temperature furnishes vinyl sulfides with high regioselectivity via anti-Markovnikov addition. The terminal alkynes provide dithiolanes at 80 °C by bis-addition. The reactions are very clean and high yielding.
Geochronological constraints on the evolution of the southern Dom Feliciano Belt (Uruguay)
Authors: Oyhantçabal Pedro , Siegesmund Siegfried , Wemmer Klaus , Presnyakov Sergey , Layer Paul 
Published: October 2009

Abstract:

New U–Pb sensitive high-resolution ion microprobe, K–Ar and Ar–Ar data from the southernmost Dom Feliciano Belt allow the identification of four major events. Orthogneisses from the Punta del Este Terrane indicate a magmatic episode at
c
. 770 Ma and high-grade metamorphism at 641 ± 17 Ma. Granitoid emplacement at 627 ± 23 Ma was roughly coeval with peak metamorphism. Volcaniclastic rocks of the Las Ventanas Formation dated at 573 ± 11 Ma can be correlated with the peripheral foreland basin (571 ± 8 Ma). Transpression and coeval high-K calc-alkaline magmatism is recorded in the Maldonado granite dated at 564 ± 7 Ma. The following events are postulated: (1) magmatism at 850–750 Ma related to rifting; (2) metamorphism and granite emplacement at 650–600 Ma; (3) molasse sequences and foreland basins at
c
. 573 Ma; (4) late magmatism at 580–560 Ma associated with transpression. The data indicate that (1) the Punta del Este Terrane could be a portion of the Coastal Terrane of the Kaoko Belt, (2) granitoid emplacement at 650–600 Ma in the Punta del Este and Nico Pérez terranes favours westward subduction, and (3) widespread post-collisional synkinematic magmatism occurred in the Dom Feliciano and Kaoko belts between 580 and 550 Ma.

Supplementary material:

Analytical methods and data are available at
http://www.geolsoc.org.uk/SUP18369
.