bluuebunny/crossref_metadata_2025_split huggingface dataset:
99mTc-labeled ubiquicidin scintigraphy
Authors: Aryana K. , Hootkani A. , Sadeghi R. , Davoudi Y. , Naderinasab M. , Erfani M. , Ayati N. 
Published: June 2012

SummaryAim: Hip prosthesis implantation has witnessed a significant increase in recent years. Despite the advantages of this surgical procedure, it has some complications, the most serious of which is prosthetic infection. This study was conducted to investigate the feasibility of 99mTc-UBI scintigraphy in detection of infectious foci in painful hip prosthesis. UBI (Ubiquicidin 29–41) is an antimicrobial peptide fragment with the ability to target the bacterial colony directly. Patients, methods: 34 patients, aged 20–79 years, with painful hip prosthesis were included. 99mTc-UBI scan and three phase bone scan were performed and two nuclear medicine specialists interpreted the UBI scans with and without bone scan results at hand. Both qualitative and semiquantitative methods were used to interpret the 30 minute post injection images. The patients were actively followed up. According to the surgical findings, microbiological culture and active follow up, final diagnosis was made. Results: 24 negative and 10 positive UBI scans were recorded. The sensitivity, specificity, negative and positive predictive values and accuracy of the study were all 100%. Bone scan did not have any influence on UBI interpretation. We were able to achieve excellent differentiation between infected and non-infected prostheses with a cut off value of 1.8 for target to non target (T/NT) ratio. No adverse effects were noticed following UBI scan. Conclusion: Based on the findings, the authors believe that 99mTc-UBI scintigraphy, with its high sensitivity and specificity, provides the physician with an excellent tool for differentiating infection from aseptic loosening of hip prostheses. Using this radiopharmaceutical, it is possible to obtain highly accurate results only 30 minutes after the beginning of the study.
Real-time pharmacy surveillance and clinical decision support to reduce adverse drug events in acute kidney injury
Authors: Cox Z.L. , Neal E.B. , Waitman L.R. , Peterson N.B. , Bhave G. , Siew E.D. , Danciu I. , Lewis J.B. , Peterson J.F. , McCoy A.B. 
Published: June 2012

Summary
Objectives: Clinical decision support (CDS), such as computerized alerts, improves prescribing in the setting of acute kidney injury (AKI), but considerable opportunity remains to improve patient safety. The authors sought to determine whether pharmacy surveillance of AKI patients could detect and prevent medication errors that are not corrected by automated interventions.
Methods: The authors conducted a randomized clinical trial among 396 patients admitted to an academic, tertiary care hospital between June 1, 2010 and August 31, 2010 with an acute 0.5 mg/dl change in serum creatinine over 48 hours and a nephrotoxic or renally cleared medication order. Patients randomly assigned to the intervention group received surveillance from a clinical pharmacist using a web-based surveillance tool to monitor drug prescribing and kidney function trends. CDS alerting and standard pharmacy services were active in both study arms. Outcome measures included blinded adjudication of potential adverse drug events (pADEs), adverse drug events (ADEs) and time to provider modification or discontinuation of targeted nephrotoxic or renally cleared medications.
Results: Potential ADEs or ADEs occurred for 104 (8.0%) of control and 99 (7.1%) of intervention patient-medication pairs (p=0.4). Additionally, the time to provider modification or discontinuation of targeted nephrotoxic or renally cleared medications did not differ between control and intervention patients (33.4 hrs vs. 30.3hrs, p=0.3).
Conclusions: Pharmacy surveillance had no incremental benefit over previously implemented CDS alerts
Comparative Study of Septoplasty Alone and with FESS in Maxillary Sinusitis with Septal Deviation
Authors: Yadav SPS , Goel Anuj Kumar , Goel Rati , Ranga Rupender , Gulia JS 
Published: June 2012

ABSTRACTIntroductionThe study was conducted to asses if septoplasty is adequate for the management of chronic maxillary sinusitis. Chronic maxillary sinusitis is not uncommonly associated with deviated nasal septum (DNS). The randomized study was conducted on 40 cases of medically unmanageable and HRCT proven chronic maxillary sinusitis with DNS.Materials and methodsThe study was conducted in 40 cases of HRCT (PNS) proven chronic maxillary sinusitis which were not cured with medical treatment. The patients were divided randomly in two groups. Group A underwent septoplasty and group B septoplasty along with FESS in the form of uncinectomy and middle meatus antrostomy. Patients having other anatomical factors for example concha were excluded.ResultsBoth procedures produced significant improvement in symptomatology and on HRCT (PNS) findings as compared to preoperative status (p-value less than 0.001). Septoplasty was found to be effective in chronic maxillary sinusitis, however, when polyps were present in maxillary sinus, then septoplasty with FESS was found to be better.DiscussionIn cases of chronic maxillary sinusitis with DNS, septoplasty is adequate, however if the maxillary sinus shows polyps on HRCT (PNS) scan then septoplasty should be combined with FESS.How to cite this articleGoel AK, Yadav SPS, Ranga R, Gulia JS, Goel R. Comparative Study of Septoplasty Alone and with FESS in Maxillary Sinusitis with Septal Deviation. Clin Rhinol Int J 2012;5(1):19-24.
The Polytrauma Patient at 34,000 Feet: The Safety of Fixed-Wing Transport
Authors: Feinstein Ara , Holland V , Sandy GL , Diaz JA , Ghaemmaghami Vafa 
Published: June 2012

ABSTRACT
The safety of fixed-wing trauma transport in critically ill trauma patients; it is not well studied. The aim of the present manuscript is to evaluate the utility and safety of this transport method when used in sick trauma patients.

Methods
A retrospective cohort analysis of 100 consecutive trauma patients transferred by a single air ambulance company was conducted. Patient information was de-identified. All patients were transported using Lear 35a jets converted for medical use. Teams consisted of a combination of physicians, nurses, respiratory therapists and paramedics.

Results
A total of 67 patients, where transported via fixed-wing in the study period. The majority of patients had blunt trauma mechanism (n = 66). The majority of flights (86.5%, n = 59) were international. The mean flight time was 3 hours 4 minutes. Mean patient contact time was 6 hours 26 minutes. Average ISS was 22. Twenty-seven percent (n = 18) were intubated, 13% (n = 9) were transfused in flight, 36% (n = 24) had head injuries and 30% (n = 20) had spine injuries. Strikingly, there were no mortalities or progression of neurologic deficits in transport.

Conclusion
Fixed-wing travel is a safe and underutilized resource in the transport of sick trauma patients.

How to cite this article
Feinstein A, Ferrada P, Holland V, Sandy GL, Diaz JA, Ghaemmaghami V. The Polytrauma Patient at 34,000 Feet: The Safety of Fixed-Wing Transport. Panam J Trauma Critical Care Emerg Surg 2012;1(1):12-14.
Outcomes of Percutaneous Cholecystostomy
Authors: Anand Rahul J , Punch Laurie , Sisley Amy C , Johnson Steven b , Lissauer Matthew 
Published: June 2012

ABSTRACT

Objective
Emergency cholecystectomy in patients with severe comorbidities carries up to 30% mortality. Percutaneous cholecystostomy (PC) is accepted as acute management in these patients. This study evaluated outcomes of PC and the need for subsequent cholecystectomy.

Methods
Retrospective chart review evaluated all patients undergoing PC between June 1, 2005 and January 1, 2010.

Results
Fifty four patients underwent PC. Indications included acute calculous cholecystitis (44%), acalculous cholecystitis (33%) and other (22%). Twelve patients had PC related complications. Seventeen patients underwent CCY 144 ± 133 days after PC placement. 71% of those procedures were converted to open operation. 15% of patients had PC tube removed successfully without cholecystectomy, 62 ± 53 days after PC. Fifteen patients died in hospital after PC, four likely related to biliary pathology. Patients who underwent subsequent cholecystectomy were more likely to have had a diagnosis of acute cholecystitis (71% vs 33%, p < 0.05). Patients with a diagnosis of acalculous cholecystitis trended toward a higher likelihood of death compared to acute cholecystitis (8 of 18, 44% vs 4 of 24, 17%, p = 0.08).

Conclusion
PC can be definitive treatment in a minority of patients with acalculous cholecystitis and severe comorbidities. Interval cholecystectomy carries a high complication rate.

How to cite this article
Ferrada PA, Anand RJ, Punch L, Sisley AC, Johnson SB, Lissauer M . Outcomes of Percutaneous Cholecystostomy. Panam J Trauma Critical Care Emerg Surg 2012;1(1):20-23.
Radiologist vs Surgeon: Misdiagnoses in Radiologic Evaluation by On-duty Surgeons in the Emergency Room
Authors: Nasr Adonis , Nakano Irene Tomoko , Rigolini Ana Valeria Brunetti , Kato Guilherme Massaaki Pianovski , de Abreu Reis Phillipe Geraldo Teixeira 
Published: June 2012

ABSTRACT

Background
Emergency department (ED) care often requires radiographs for denitive diagnosis. In many hospitals the primary review of examinations is by requesting physicians. We studied the importance of having a radiologist assess tests, by assessing diagnostic disagreement.

Study design
Retrospective, observational, involving victims of trafc injuries (August 2008). Trauma routine X-rays were analyzed by treating surgeon and by the radiologist at the same hospital. Comparison and review of their reports.

Results
Fourty-seven lesions were found on cervical, chest and pelvis radiographs. Twenty-three were considered as resulting from trafc injury. Other 24 were chronic injuries or ndings with no connection to the mechanism of trauma. Regarding the rst group, surgeons identied 19 lesions (82.61%), while the radiologist found 22 (95.66%). There was one lesion described only by the doctor on duty, but not by the radiologist (rib fracture). Four lesions were not identied by the ED surgeons, but only by the radiologist (two fractures: one of the upper limb and another of the clavicle; a pneumothorax and a subcutaneous emphysema). There was a discrepancy of 13.05% between the diagnosed injuries. All lesions misdiagnosed were found on chest X-rays.

Conclusion
We found a significant rate of radiographic injuries caused by trauma misdiagnosed by the on-duty doctor (13.05%). This nding points to the need for an on-duty radiologist for evaluation of tests in the emergency department.

How to cite this article
Nasr A, Nakano IT, Rigolini AVB, Kato GMP, de Abreu Reis PGT. Radiologist vs Surgeon: Misdiagnoses in Radiologic Evaluation by On-duty Surgeons in the Emergency Room. Panam J Trauma Critical Care Emerg Surg 2012;1(1):38-43.
Ultra-Performance Liquid Chromatography for the Multicomponent Analysis of a Ternary Mixture Containing Thiamine, Pyridoxine, and Lidocaine in Ampules
Authors: Dinç Erdal , Baleanu Dumitru 
Published: June 2012

Abstract
New chemometric approaches based on the application of partial least squares (PLS) and principal component regression (PCR) algorithms with fractional wavelet transform (FWT) and continuous wavelet transform (CWT) are proposed for the spectrophotometric multicomponent determination of thiamine hydrochloride (B1), pyridoxine hydrochloride (B6), and lidocaine hydrochloride (LID) in ampules without any separation step. In this study PLS and PCR techniques were applied to the raw spectral data, FWT-coefficients, and FWT-CWT-coefficients. These calibration models were labeled as Raw-PLS and Raw-PCR, FWT-PLS and FWT-PCR, and FWT-CWT-PLS and FWT-CWT-PCR, respectively. A new ultra-performance liquid chromatographic (UPLC) method was developed for the comparison of the results obtained by applying the chemometric calibration methods. Chromatographic separation and determination of B1, B6, and LID in ampules were performed on an Acquity UPLC® BEH C18 column (50 × 2.1 mm id, 1.7 μm particle size) using gradient elution with a mobile phase consisting of methanol and 0.01 M HCl at a constant flow rate of 0.6 mL/min. These combined chemometric calibrations and UPLC were validated by analyzing various ternary mixtures, B1, B6, and LID. The proposed chemometric approaches (signal processing-multivariate calibrations) and UPLC method were applied to the quantitative multicomponent analysis of marketed ampules containing the vitamins B1 and B6 with LID.
Determination of the Isoflavone Genistein in Soybeans by High-Performance Liquid Chromatography Following Cloud Point Extraction
Authors: Mirzaei Mohammad , Naeini Atieh Karimi , Behzadi Mansoureh 
Published: June 2012

Abstract
A simple and sensitive method has been developed for preconcentration and determination of genistein in soybeans. This method is based on cloud point extraction (CPE) of genistein from soybeans using ethylene glycol monoalkyl ether (Genapol X-080) as a nonionic surfactant. The concentration of extracted genistein was determined by HPLC with a UV detector. Optimum experimental conditions were established. With 5% Genapol X-080 (v/v), a liquid/solid ratio of 25:1 mL/g, and ultrasonic-assisted extraction at 40°C for 45 min, the extraction percentage of genistein reached its highest value. The preconcentration factor for genistein was about 16.5. The RSD for seven replicate measurements and the LOD were ±4.45% and 15.0 ng/mL, respectively. CPE is simple, inexpensive, and suitable for extraction of genistein from soybean. It uses environmentally friendly surfactants and offers a convenient alternative to more conventional extraction systems.
SDIX RapidChek™ Listeria F.A.S.T.™ Environmental Test System for the Detection of Listeria species on Environmental Surfaces
Authors: Muldoon Mark T , Allen Anne-Christine Olsson , Gonzales Vera , Sutzko Meredith , Lindpaintner Klaus 
Published: June 2012

Abstract
The SDIX RapidChekTMListeria F.A.S.T. test system was validated against the U. S. Department of Agriculture-Food Safety and Inspection Service (USDA-FSIS) cultural reference method for the detection of Listeria species on stainless steel, plastic, rubber, and painted concrete. The SDIX method uses a proprietary RapidChek Listeria enrichment media for a one-step, 24–40 h enrichment at 30°C, and detects Listeria on an immunochromatographic lateral flow device in 10 min. Different Listeria species were used to spike each of the environmental surfaces. Environmental surfaces were spiked at levels ranging from 50 to 400 CFU/surface (1 in.2 swabs for painted concrete, 4 in.2 for sponge). A total of 120 spiked samples were tested by the SDIX method at 24 and 40 h and the cultural reference method. Total confirmed positives were 49, 54, and 48 for the SDIX 24 h method, the SDIX 40 h method, and the USDA-FSIS cultural reference method, respectively. Nonspiked samples from all environmental surfaces were reported as negative for Listeria spp. by all methods. The overall Chi square was 0.017 (P = 0.104) and 0.611 (P = 0.566) after a 24 and 40 h enrichment, respectively, indicating that the test method was equivalent in performance to the reference method at both enrichment times. The SDIX method was evaluated for the detection of 50 Listeria and 35 non-Listeria bacterial strains. All 50 Listeria strains were detected by the method (100% sensitivity). Five out of 35 non-Listeria species gave light test signals when grown in nonselective broth culture and tested undiluted. However, when grown in the RapidChek Listeria F.A.S.T. proprietary media, only one bacterial strain (Staphylococcus aureus) was detected, giving a very low test signal (97% specificity). The method was shown to be robust toward several alterations in testing and storage conditions.
RAZOR® EX Anthrax Air Detection System
Authors: Spaulding Usha K , Christensen Clarissa J , Crisp Robert J , Vaughn Michael B , Trauscht Robert C , Gardner Jordan R , Thatcher Stephanie A , Clemens Kristine M , Teng David H , Bird Abigail , Ota Irene M , Hadfield Ted , Ryan Valorie , brUnelle sharon l 
Published: June 2012

Abstract
The RAZOR® EX Anthrax Air Detection System, developed by Idaho Technology, Inc. (ITI), is a qualitative method for the detection of Bacillus anthracis spores collected by air collection devices. This system comprises a DNA extraction kit, a freeze-dried PCR reagent pouch, and the RAZOR EX real-time PCR instrument. Each pouch contains three assays, which distinguish potentially virulent B. anthracis from avirulent B. anthracis and other Bacillus species. These assays target the pXO1 and pXO2 plasmids and chromosomal DNA. When all targets are detected, the instrument makes an “anthrax detected” call, meaning that virulence genes of the anthrax bacillus are present. This report describes results from AOAC Method Developer (MD) and Independent Laboratory Validation (ILV) studies, which include matrix, inclusivity/exclusivity, environmental interference, upper and lower LOD of DNA, robustness, product consistency and stability, and instrument variation testing. In the MD studies, the system met the acceptance criteria for sensitivity and specificity, and the performance was consistent, stable, and robust for all components of the system. For the matrix study, the acceptance criteria of 95/96 expected calls was met for three of four matrixes, clean dry filters being the exception. Ninety-four of the 96 clean dry filter samples tested gave the expected calls. The nucleic acid limit of detection was 5-fold lower than AOAC's acceptable minimum detection limit. The system demonstrated no tendency for false positives when tested with Bacillus cereus. Environmental substances did not inhibit accurate detection of B. anthracis. The ILV studies yielded similar results for the matrix and inclusivity/exclusivity studies. The ILV environmental interference study included environmental substances and environmental organisms. Subsoil at a high concentration was found to negatively interfere with the pXO1 reaction. No interference was observed from the environmental organisms. The nucleic acid LOD, however, was 10 times higher (1 pg/reaction, equivalent to about 200 spores) than that found in the MD study. These results indicate that the RAZOR System is a sensitive and specific system that accurately identifies B. anthracis in aerosol matrixes and in the presence of interfering substances, and that the method can be performed by an independent laboratory and achieve similar results.
Application of Inductively Coupled Plasma/Mass Spectrometry for the Measurement of Chromium, Selenium, and Molybdenum in Infant Formula and Adult Nutritional Products: First Action 2011.19
Authors: Pacquette Lawrence H , Szabo Andre , Thompson Joseph J , Baugh Steve 
Published: June 2012

Abstract
An inductively coupled plasma/MS method was developed for the simultaneous determination of Cr, Se, and Mo in infant formula and other nutritional products. All samples were digested using a closed vessel microwave oven system, together with Ni and Te internal standards. The practical quantitation limits for Cr, Se, and Mo were 0.4, 0.2, and 0.4 ng/mL, respectively; dilution factors were 250 for powders and 50 for liquids. The Cr, Se, and Mo concentrations in 10 nutritional products were within specification limits; within-day and day-to-day (6 independent days) precision values were &lt;5% RSD. For two control samples, the observed precision was ≤2% RSD over 10 independent days. Cr, Se, and Mo results were within the certified limits in three National Institute of Standards and Technology standard reference materials. The average sample spike recoveries for 10 nutritional products ranged from 93 to 107%. Robustness studies showed a minimal effect from concomitant easily ionized element concentrations. However, the choice of internal standard and matrix-matching carbon content were critical to obtaining accurate Se results. All indications are that this method would be a suitable candidate as a global reference method for the determination of these trace elements in infant formula, adult nutritionals, and other nutritional products.
Feature Selection Methods in QSAR Studies
Authors: Goodarzi Mohammad , Dejaegher Bieke , Heyden Yvan Vander 
Published: June 2012

Abstract
A quantitative structure-activity relationship (QSAR) relates quantitative chemical structure attributes (molecular descriptors) to a biological activity. QSAR studies have now become attractive in drug discovery and development because their application can save substantial time and human resources. Several parameters are important in the prediction ability of a QSAR model. On the one hand, different statistical methods may be applied to check the linear or nonlinear behavior of a data set. On the other hand, feature selection techniques are applied to decrease the model complexity, to decrease the overfitting/overtraining risk, and to select the most important descriptors from the often more than 1000 calculated. The selected descriptors are then linked to a biological activity of the corresponding compound by means of a mathematical model. Different modeling techniques can be applied, some of which explicitly require a feature selection. A QSAR model can be useful in the design of new compounds with improved potency in the class under study. Only molecules with a predicted interesting activity will be synthesized. In the feature selection problem, a learning algorithm is faced with the problem of selecting a relevant subset of features upon which to focus attention, while ignoring the rest. Up to now, many feature selection techniques, such as genetic algorithms, forward selection, backward elimination, stepwise regression, and simulated annealing have been used extensively. Swarm intelligence optimizations, such as ant colony optimization and partial swarm optimization, which are feature selection techniques usually simulated based on animal and insect life behavior to find the shortest path between a food source and their nests, recently are also involved in QSAR studies. This review paper provides an overview of different feature selection techniques applied in QSAR modeling.
Chemometric Processing of Pharmaceutical Essential Oil Fingerprints—Comparison of GC, HPLC, TLC, IR Spectroscopy, and Differential Scanning Calorimetry
Authors: Pietraś Rafał , Skibiński Robert , Trębacz Hanna , Gumieniczek Anna 
Published: June 2012

Abstract
Fifteen essential oils of pharmaceutical grade were fingerprinted by five techniques: TLC, GC, HPLC, attenuated total reflectance FTIR spectroscopy, and differential scanning calorimetry (DSC). Denoising and baseline removal was found to be a crucial step for correct comparative analysis. Standardization of the signal was not necessary in the presented case; however, it should be considered and checked in each case. Due to small variance explained by first two principal components (below 50%) and outlying observations, the main analysis was performed by Euclidean dendrograms. It was found that almost all techniques besides DSC find real chemical similarities; however, DSC can be used as an additional tool. The similarities among the five techniques were also compared and discussed.