bluuebunny/crossref_metadata_2025_split huggingface dataset:
A pilot study on the antimicrobial efficacy of Coleus Aromaticus on Lactobacillus acidophilus – Comparative In-Vitro analysis of five samples on a single plate
Authors: Krishnan B. Sai , Chakravarthy Yadav , Jacob Mathew 
Published: May 2024

Objectives:
Coleus aromaticus, also known as Indian borage, is a plant recognized for its medicinal uses, including potential antimicrobial properties. Lactobacillus acidophilus, a bacterium commonly found in the oral cavity and gastrointestinal tract, plays a crucial role in maintaining microbial balance but can lead to dental caries if imbalanced. The aim of this study is to assess the in vitro antimicrobial efficacy of C. aromaticus against L. acidophilus.

Material and Methods:
The study was performed by the utilization of homeopathic ethanolic and dry leaf extracts of C. aromaticus on Lactobacillus bacteria, with a control group treated with 0.2% chlorhexidine. For the preparation of the C. aromaticus extract, leaves were cleaned, dried, and ground into a paste. A 20 g portion of this paste was then weighed and used for extraction. In the assessment of inhibitory effects, the disc diffusion method was employed, and nutrient agar served as the growth medium. Discs loaded with the respective extracts were positioned on the agar, and the Petri dishes were subsequently incubated at 37°C for 24 h. Following the incubation period, the inhibitory zones surrounding the discs were measured using a measuring scale.

Results:
The study’s findings were determined by measuring the diameter of inhibition zones and assessing the mean values. It was observed that the aqueous extract derived from dried C. aromaticus leaves possesses antimicrobial activity against Lactobacillus. Specifically, it exhibited inhibition zones with diameters of 24 mm and 27 mm when using 100 µL/disc and 200 µL/disc, respectively. In comparison, the control group (0.2% chlorhexidine) displayed an inhibitory zone with a 23 mm diameter.

Conclusion:
The reason for these findings could be attributed to the existence of phytochemicals and essential volatile oils within C. aromaticus, which possess antimicrobial properties against Lactobacillus. This natural component appears to be effective in reducing Lactobacillus growth when used as an oral rinse containing phytochemicals.
Pengaruh Pelatihan dan Disiplin Kerja Terhadap Kinerja Karyawan
Authors: Fangiziah Elsya Azkiyathul , Agung Syahrum , Nurhayati Immas 
Published: May 2024

This study aims to determine the effect of training and work discipline on the performance of PT. Vallian Lestari Bersama. This study used a quantitative approach with data collection techniques through the distribution of questionnaires or questionnaires. The population in this study is all employees at PT. Vallian Lestari Bersama totaled 50 people, the samples used were 44 people taken using the slovin formula with a precision level of 5%. Data analysis methods used in this study include validity and reliability tests, classical assumption tests, correlation analysis, multiple linear regression analysis, and t and F hypothesis tests processed using the SPSS 25 application. The results showed that there was a strong correlation between training and work discipline on employee performance. The results of the effect of training on employee performance obtained a calculated t value of 6.503 > t table 2.020 with a significant value of 0.000 < 0.05, it can be stated that there is a partial significant influence between training on employee performance, the results of the influence of work discipline on employee performance obtained a calculated t value of 5.550 > ttable 2.020 with a significant value of 0.000 < 0.05, it can be stated that there is a partial significant influence between work discipline on employee performance, the results of the effect of training and work discipline on employee performance obtained a calculated F value of 24.829 > Ftable 3.22 with a significant value of 0.000 < 0.5 so that it can be concluded that there is a simultaneous significant influence between training and work discipline on employee performance of PT. Vallian Lestari Bersama.
Evaluasi Beban Kerja Mental Karyawan Dengan Menggunakan Metode DRAWS dan RSME
Authors: Adelino Muhammad Ilham , Harma Beni , Afrianda Best 
Published: May 2024

PT. KLG merupakan perusahaan yang bergerak mengolah hasil perkebunan karet yang dipasok dari supplier, dimana hasil produksi berupa karet basah dan karet remah (crumb rumber) akan dipasarkan ke industri internasional. Sistem kerja shift yang panjang menjadi salah satu masalah utama terjadinya beban kerja mental karyawan di PT. KLG . Penelitian ini bertujuan untuk mengetahui seberapa besar beban kerja mental yang dialami karyawan divisi produksi PT. KLG saat bekerja. Pengambilan data melalui kusioner dilakukan untuk mengetahui seberapa besar beban kerja yang dirasakan oleh karyawan yang kemudian dihitung dengan menggunakan metode Defence Research Agency Workload Scale (DRAWS) dan Rating Scale Mental Efford (RSME). Berdasarkan skor akhir penentuan beban kerja mental dengan metode DRAWS diketahui bahwa pekerja memilki beban kerja mental yang tinggi yaitu dengan skor 77.4% dengan kategori overload. Begitupun dari perhitungan beban kerja mental menggunakan metode RSME diketahui besarnya usaha yang dilakukan oleh pekerja termasuk dalam kategori besar dengan skor 93,08. Penambahan shift kerja menjadi 2 shift perlu dilakukan agar mengurangi beban kerja mental karyawan sehingga berada dalam kategori underload.
Students' Problem-Solving Ability Using Picture Story Contexts
Authors: Lisnani  , Inharjanto Anselmus 
Published: May 2024

Kemampuan pemecahan masalah matematis menjadi salah satu komponen yang perlu dianalisis untuk mengetahui kemampuan peserta didik terhadap satu topik pembelajaran. Penelitian ini bertujuan untuk menganalisis kemampuan pemecahan masalah matematis peserta didik pada materi bangun datar, pengukuran panjang dan berat. Metode penelitian yang digunakan adalah kualitatif deskriptif. Instrumen pengumpulan data melalui tes, kuesioner, dan dokumentasi. Keseluruhan data tersebut dianalisis dan dideskrispsikan oleh peneliti. Subjek dalam penelitian ini adalah peserta didik kelas IV di salah satu sekolah swasta di Palembang berjumlah 14 orang yang diperoleh melalui purposive sampling. Hasil dari penelitian ini adalah kemampuan pemecahan masalah pada materi bangun datar dan pengukuran pada peserta didik kelas IV di salah satu sekolah swasta tersebut sebesar 75,59% yang artinya berkategori baik. Kemampuan pemecahan masalah peserta didik kelas IV perlu ditingkatkan terutama pada sesuatu yang sifatnya konkret dengan membiasakan peserta didik memberikan soal-soal berkonteks cerita yang mengarah ke AKM, PISA, dan HOTS kedepannya.

Mathematical problem-solving ability is one of the necessary components to analyze as it demonstrates students' ability to comprehend a learning topic. This study aimed to analyze students' mathematical problem-solving skills on flat shapes, measuring length and weight. This descriptive qualitative study used tests, questionnaires, and document analysis to collect the data. All of the data were analyzed and described by the researcher. The participants of the study were 14 fourth grade students in a private school in Palembang selected through purposive sampling technique. The study revealed that the students’ problem-solving abilities on flat shapes and measurements scored 75.59%, which was categorized as “good”. The problem-solving abilities of fourth graders need to be improved, especially on concrete objects by inuring students to story-context questions that promote AKM, PISA, and HOTS in the future.
Description of Hemoglobin Levels in Prospective Donors Over 46 Years Old at UDD PMI Jepara Regency
Authors: Luthfia Alya , Wikandari Ririh Jatmi , Widodo Widodo 
Published: May 2024

Blood donation is the process of taking part of blood from the body of a healthy person and given to people who need blood voluntarily (Ibrahim et al., 2021). Hemoglobin level examination is one of the donor selection checks to find out whether someone is successful or not to donate blood. A person can donate blood if the hemoglobin level shows a result of 12.5 g / dL-17 g / dL. The factor causing the rejection of most blood donations in Indonesia is due to low hemoglobin levels (Putri Afrianti, 2022). The case of high Hb levels is also one of the factors that causes rejection in donor selection (Nuraini Kwarta, 2021). The levels and activity of substances in the blood can be affected by age. Neonates have higher Hb levels than adults due to loss of active tissue mass and many people whose function has been reduced, resulting in reduced Hb levels (Paramahita et al., 2020). The purpose of this study was to measure hemoglobin levels in prospective donors aged more than 46 years at UDD PMI Jepara Regency. This type of research uses a quantitative approach with a cross-sectional descriptive study research model taken from the SIMDONDAR database. Based on the results of the study, it was found that the majority of prospective donors showed normal hemoglobin levels as many as 480 prospective donors (94.86%), and more prospective donors who had blood type O (+) as many as 187 prospective donors (36.96%), most prospective donors were male as many as 342 prospective donors (67.59%), and the majority of prospective donors were declared to have passed the donor selection as many as 480 prospective donors (94.86%).
Experimental Study on Compressive Strength of Concrete Under Simple Curing and Encased in Steel Mould
Authors: Dai Xianghe 
Published: May 2024

This paper presents a comparison and analysis of compressive strength development of normal concrete under three typical simple curing methods: air curing, water immersion curing, plastic film cover curing, and maintained in steel mould. Portland cement with 30% fly ash was used, concrete cubic compressive strength at 28 days and 60 days were tested. The comparison of experimental results shows that concrete samples applied 7-day water immersion curing and samples maintained in steel moulds up to testing date displayed excellent compressive strength development compared with concrete samples under air curing. This implies core concrete in steel tubular members might develop a higher strength than design assumption based on standard curing procedure. Wrapping concrete with plastic film for more than 7 days as a curing method might delay the concrete strength development in early stage. Water immersion curing for long period may not accelerate concrete compressive strength gain.
Penguatan Keterampilan Pengelolaan Wihara bagi Pengurus Wihara-Wihara di Kabupaten Lampung Timur
Authors: Kunarso Kunarso , Subekti Puja , Sapardi Sapardi , Putra Edi Ramawijaya , Heriyanto Heriyanto , Suwarni Iin , Dharma Andhika Mustika , Widiyono Eling 
Published: May 2024

ABSTRAK Keterampilan pengelolaan wihara merupakan hal yang perlu terus dilakukan bagi pengurus ataupun calon pengurus. Hal ini dikarenakan pengurus merupakan garda terdepan dalam menciptakan eksistensi sebuah organisasi. Selain itu wihara sebagai tempat ibadah juga dituntut untuk terus berkembang guna membantu masyarakat tidak hanya dalam bidang agama namun juga dibidang-bidang lainnya seperti ekonomi, sosial, ataupun kesehatan. Kegiatan Pengabdian kepada Masyarakat ini bertujuan untuk meningkatkan pemahaman terkait keterampilan pengelolaan wihara yang diharapkan akan menunjang tercapainya visi, misi, serta tujuan organisasi. Kegiatan ini dilakukan dengan menggunakan metode penyuluhan yang terbagi menjadi beberapa tahap yakni persiapan, pelaksanaan, dan evaluasi. Kegiatan ini dilaksanakan di Wihara Buddha Dipa dengan peserta berjumlah 30 orang yang merupakan pengurus wihara di Kabupaten Lampung Timur. Hasil kegiatan menunjukkan bahwa peserta mengalami peningkatan pemahaman berkaitan dengan pemahaman sebesar 87,5%. Secara keseluruhan kegiatan berlangsung dengan lancar meskipun terdapat beberapa kendala yang bersumber dari aspek internal ataupun eksternal akan tetapi dapat teratasi dengan baik. Kata Kunci: Keterampilan, Manajemen, Pengelolaan, Wihara ABSTRACT Temple management skills are something that needs to be done continuously for administrators or prospective administrators. This is because the board is the front line in creating the existence of an organization. In addition, the temple as a place of worship is also required to continue to grow to help the community not only in the field of religion but also in other fields such as economic, social, or health. This Community Service activity aims to increase understanding of temple management skills that are expected to support the achievement of the organization's vision, mission, and goals. This activity is carried out using counseling methods which are divided into several stages, namely preparation, implementation, and evaluation. This activity was carried out at Dipa Temple with 30 participants who were temple administrators in East Lampung Regency. The results of the activity showed that participants experienced an increase in understanding related to understanding by 87.5%. Overall, the activity ran smoothly even though there were several obstacles originating from internal or external aspects, but it could be overcome properly. Keywords: Skills, Management, Management, Temple
A novel diagnostic approach to Paratuberculosis in dairy cattle using near-infrared spectroscopy and aquaphotomics
Authors: Behdad Saba , Pakdel Abbas , Massudi Reza 
Published: May 2024

IntroductionAs a contagious and chronic disease in the livestock industry, Paratuberculosis is a significant threat to dairy herds’ genetic and economic resources. Due to intensive breeding and high production of dairy cattle, the incidence and prevalence are higher. Developing non-destructive diagnostic methods for the early detection and identification of healthy animals is paramount for breeding programs. Conventional methods are almost entirely destructive, have low accuracy, lack precision, and are time-consuming. Near-infrared spectroscopy (NIRS) and aquaphotomics can detect changes in biofluids and thus have the potential to diagnose disease. This study aimed to investigate the diagnostic ability of NIRS and aquaphotomics for Paratuberculosis in dairy cattle. MethodsBlood plasma from dairy cattle was collected in the NIR range (1,300 nm to 1,600 nm) 60 days before and 100 days to 200 days after calving in two groups, positive and negative, using the same consecutive enzyme-linked immunosorbent assay test results three times as a reference test.ResultsNIRS and aquaphotomics methods invite 100% accuracy, sensitivity, and specificity to detect Paratuberculosis using data mining by unsupervised method, Principal Component Analysis, and supervised methods: Soft Independent Modeling of Class Analogiest, Linear Discriminant Analysis, Quadratic Discriminant Analysis, Partial Least Square–Discriminant Analysis, and Support Vector Machine models.DiscussionThe current study found that monitoring blood plasma with NIR spectra provides an opportunity to analyze antibody levels indirectly via changes in water spectral patterns caused by complex physiological changes, such as the amount of antibodies related to Paratuberculosis by aquagram.
The genetic relationships between immune cell traits, circulating inflammatory proteins and preeclampsia/eclampsia
Authors: Liu Yu , Zhang Yuliang , Du Lili , Chen Dunjin 
Published: May 2024

ObjectivesPreeclampsia/eclampsia (PE), a critical complication during pregnancy, has been suggested to correlate with immune cell phenotypes and levels of circulating inflammatory proteins. Our study aimed to employ a two-sample mendelian randomization (MR) analysis to assess the potential causal effects of immune cell phenotypes and circulating inflammatory proteins on the onset of PE.MethodsWe utilized summary-level data from genome-wide association studies (GWAS). This included statistics for 371 immune cell phenotypes from 3,757 individuals in the Sardinian founder population, and data on 91 circulating inflammatory proteins from 14,824 European ancestry participants. Additionally, genetic associations related to PE were extracted from the FinnGen consortium, involving 1,413 cases and 287,137 controls. We applied inverse variance weighting (IVW) and supplementary methods like MR-Egger, weighted median, and weighted mode to comprehensively assess potential causal links.ResultsOur analysis revealed significant causal associations of several immune cells type and inflammatory proteins with PE. Out of the immune cell phenotypes analyzed, six immune phenotypes emerged as significant risk factors (p &lt;0.01), mainly include CD4 on activated and secreting CD4 regulatory T cells, CD28 on CD39+ CD4+ T cells, CD127- CD8+ T cell absolute cell (AC) counts, HLA DR on HLA DR+ CD8+ T cell, CD66b on CD66b++ myeloid cells, and HLA DR on dendritic cells. And ten were identified as protective factors (p &lt;0.01). Such as CD45 on CD33br HLA DR+ CD14-, CD33+ HLA DR+ AC, CD33+ HLA DR+ CD14- AC, CD33+ HLA DR+ CD14dim AC, CD27 on CD24+ CD27+ B cell, CD20- CD38- %B cell, IgD- CD24- %B cell CD80 on plasmacytoid DC, CD25 on CD4+ T cell, and CD25 on activated &amp; secreting CD4 regulatory T cell. Furthermore, among the inflammatory proteins studied, five showed a significant association with PE, with three offering protective effects mainly include that C-X-C motif chemokine 1, tumor necrosis factor ligand superfamily member 14, and C-C motif chemokine 19 and two exacerbating PE risk such as STAM-binding domain and Interleukin-6 (p &lt;0.05).ConclusionsOur study highlights the pivotal roles played by diverse immune cell phenotypes and circulating inflammatory proteins in the pathophysiology of PE. These findings illuminate the underlying genetic mechanisms, emphasizing the criticality of immune regulation during pregnancy. Such insights could pave the way for novel intervention strategies in managing PE, potentially enhancing maternal and neonatal health outcomes.
Clinical characteristics and prognostic factors of COVID-19 infection among cancer patients during the December 2022 – February 2023 Omicron variant outbreak
Authors: Liu Li-Li , Liao Yu-Wei , Yu Xiao-Hua , Rong Ling , Chen Bi-Gui , Chen Gang , Zeng Guang-Kuan , Yang Li-Ye 
Published: May 2024

ObjectiveTo analyze the clinical characteristics and prognostic impacts of SARS-CoV-2 Omicron infection among cancer inpatients during the December 2022 – February 2023 surge, in order to provide scientific evidence for clinical treatment and prevention and control measures.MethodsA retrospective analysis was conducted on the clinical features, prognosis, and vaccination status of cancer in-patients infected with the Omicron variant during the COVID-19 pandemic of December 2022 – February 2023.ResultsA total of 137 cancer inpatients were included in the study, with a median age of 61 years, and 75 patients (54.74%) were male. The main symptoms were cough (69 cases, 50.36%), expectoration (60 cases, 43.80%), and fever (53 cases, 39.69%). Chest CT examination revealed bilateral pneumonia in 47 cases (34.31%, 47/137) and pleural effusion in 24 cases (17.52%, 24/137). Among the cancer patients, 116 cases (84.67%, 116/137) had solid tumors, and 21 cases (15.33%, 21/137) had hematologic malignancies, with the main types being breast cancer (25 cases, 18.25%) and lung cancer (24 cases, 17.52%). Among the cancer patients, 46 cases (33.58%) were asymptomatic, 81 cases (59.12%) had mild disease, 10 cases (7.30%) had severe infection, and 8 cases (5.84%) died. A total of 91 patients (66.42%) had been vaccinated, with 58 patients (42.34%) receiving three doses. Multivariate analysis showed that cerebral infarction and hypoproteinemia were risk factors for death from COVID-19 infection.ConclusionCancer patients infected with SARS-CoV-2 Omicron typically exhibit mild disease manifestations, but some cancer patients infected with the Omicron variant might progress to severe illness, and even death, necessitating close monitoring and attention during the early stages of infection. Additionally, the presence of cerebral infarction and hypoproteinemia significantly increases the risk of death.
Clinical characteristics and genetic analysis of children with Omicron BF.7.14 type novel coronavirus-related acute necrotizing encephalopathy
Authors: Zhang Jianzhao , Sun Jing , Li Dongqing , Xie Hua , Shangguan Shaofang , Ge Yangyang , Jiao Hui , Ma Caihui , Liu Zhao , Zhao Linqing , Yang Jian 
Published: May 2024

BackgroundTo explore the clinical characteristics, etiological factors, and clinical-related genetic variant of children with acute necrotizing encephalopathy (ANE) related to the Omicron BF.7.14 novel coronavirus.MethodsGenomic variations were detected through whole exome sequencing. Additionally, we summarized the clinical data to explore the inheritance patterns associated with novel coronavirus-related ANE.ResultsThis study included four patients (2 males and 2 females) with an average age of 2.78 ± 1.93 years. All the patients had prodromal symptoms of Omicron BF.7.14 virus infection, and exhibited symptoms such as altered consciousness, seizures and cognitive/language disturbances. Cranial MRI scans revealed damage to the thalamus, basal ganglia and brainstem. The cerebrospinal fluid (CSF) cell counts were nearly normal, but protein level in CSF increased significantly. Genetic analysis revealed a novel truncated variant of CRMP2 gene in one patient who suffered more severe coma score and prognosis and dead in the later stages. All children exhibited a decrease in the absolute count of T lymphocytes, helper T cells, suppressor T cells, and NK cells to varying degrees. Furthermore, levels of cytokines, including IL-1 β, IL-5, IL-6 and IL-8 were significantly elevated in the CSF, especially in patient with truncated variant of CRMP2 gene.ConclusionThe Omicron BF.7.14 type novel coronavirus can lead to ANE, characterized by T cell immunosuppression and a significant increase in cytokine levels in the CSF. The truncated variation of CRMP2 gene may affect the prognosis of ANE by affecting the migration of cerebral T cells.