bluuebunny/crossref_metadata_2025_split huggingface dataset:
Calcium phosphate‐based composites as injectable bone substitute materials
Authors: Low Kah Ling , Tan Soon Huat , Zein Sharif Hussein Sharif , Roether Judith A. , Mouriño Viviana , Boccaccini Aldo R. 
Published: March 2010

AbstractA major weakness of current orthopedic implant materials, for instance sintered hydroxyapatite (HA), is that they exist as a hardened form, requiring the surgeon to fit the surgical site around an implant to the desired shape. This can cause an increase in bone loss, trauma to the surrounding tissue, and longer surgical time. A convenient alternative to harden bone filling materials are injectable bone substitutes (IBS). In this article, recent progress in the development and application of calcium phosphate (CP)‐based composites use as IBS is reviewed. CP materials have been used widely for bone replacement because of their similarity to the mineral component of bone. The main limitation of bulk CP materials is their brittle nature and poor mechanical properties. There is significant effort to reinforce or improve the mechanical properties and injectability of calcium phosphate cement (CPC) and this review resumes different alternatives presented in this specialized literature. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Repairing goat tibia segmental bone defect using scaffold cultured with mesenchymal stem cells
Authors: Liu Xinhui , Li Xiaoming , Fan Yubo , Zhang Guoping , Li Dongmei , Dong Wei , Sha Ziyi , Yu Xiaoguang , Feng Qingling , Cui Fuzhai , Watari Fumio 
Published: March 2010

AbstractIn this study, we investigated cellular biocompatibility in vitro and segmental bone defect repairing efficacy in vivo of a previously reported fibre‐reinforced scaffold, nano‐hydroxyapatite/collagen/poly (L‐lactic acid) (PLLA)/chitin fibres (nHACP/CF). First, attachment, proliferation, and differentiation of the goat bone mesenchymal stem cells (GBMSCs) cultured on the nHACP/CF scaffolds were evaluated in vitro. The results showed that cells attached to the scaffolds well, and there was no significant difference in cell proliferation between cells on the scaffolds and cells on the polystyrene culture plates that were used as a control. The results also showed that alkaline phosphatase (ALP)/DNA of the cells cultured on the scaffolds was significantly higher than that on the control. The in vivo study compared the bone defect repairing efficacy of nHACP/CF scaffolds with that of autograft bone. Thirty‐two adult male goats with 25‐mm defects in their tibias at the same anatomic site were divided into four groups. The first group was implanted with the nHACP/CF with GBMSCs. The second group was implanted with autograft bone. The third group was implanted with the nHACP/CF. Nothing was implanted in the fourth group. Bone growth was evaluated by radiography, histology, and biomechanics. The results showed that although the nHACP/CF had new bone formation, it could not repair the defect fully while nHACP/CF with GBMSCs cultured and autograft bone could repair the segmental bone defect by 8 weeks after surgery, suggesting that nHACP/CF is an appropriate scaffold for bone tissue engineering. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Injectable bone cement based on mineralized collagen
Authors: Liu Xi , Wang Xiu‐Mei , Chen Zonggang , Cui Fu‐Zhai , Liu Huan‐Ye , Mao Keya , Wang Yan 
Published: March 2010

AbstractA novel injectable bone cement based on mineralized collagen was reported in this paper. The cement was fabricated by introducing calcium sulfate hemihydrate (CaSO4·1/2H2O, CSH) into nano‐hydroxyapatite/collagen (nHAC). The workability, in vitro degradation, in vitro and in vivo biocompatibility of the cement (nHAC/CSH) were studied. The comparative tests via in vitro and in vivo showed that the nHAC/CSH composite cement processed better biocompatibiltiy than that of pure CSH cement. The results implied that this new injectable cement should be very promising for bone repair. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Cross‐talk between T‐Ag presence and pRb family and p53/p73 signaling in mouse and human medulloblastoma
Authors: Caracciolo Valentina , Macaluso Marcella , D'Agostino Luca , Montanari Micaela , Scheff Jonathan , Reiss Krzysztof , Khalili Kamel , Giordano Antonio 
Published: March 2010

AbstractThe formation and progression of mudulloblastoma (MB) is poorly understood. However, somatic inactivation of pRb/p105, in combination with a somatic or a germ‐line TP53 inactivation, leads to MB in a mouse model. Presently, there is no specific evidence of pathway/s alterations for the other two members of the retinoblastoma family, pRb2/p130 and/or p107 in MB. JC virus (JCV) is a human polyomavirus. Although there is no firm evidence that this virus plays a causal role in human neoplasia, it has been clearly proven that JCV is highly oncogenic when injected into the brain of experimental animals. The mechanism of JCV‐induced tumorigenesis is not entirely clear. However, several studies relate the oncogenic properties of JCV mainly to its early protein large T‐antigen (T‐Ag), which is able to bind and inactivate both TP53 and Rb family proteins. Here, we compared the protein expression profiles of p53, p73, pRb family proteins, and PCNA, as main regulators of cell proliferation and death, in different cell lines of mouse primitive neuroectodermal tumors (PNET), either T‐Ag‐positive or ‐negative, and in human MB cell lines. Our goal was to determine if changes in the relative expression of these regulators could trigger molecular perturbations underlying MB pathogenesis in mouse and human cells. Our results support that the presence of JCV T‐Ag may interfere with the expression of pRb family proteins, specific p73 isoforms, and p53. In turn, this “perturbation” may trigger a network of signals strictly connected with survival and apoptosis. J. Cell. Biochem. 110: 182–190, 2010. © 2010 Wiley‐Liss, Inc.
Effect of a synthetic cannabinoid agonist on the proliferation and invasion of gastric cancer cells
Authors: Xian Xiang‐Shu , Park Hyeyeon , Cho Yu Kyung , Lee In Seok , Kim Sang Woo , Choi Myung‐Gyu , Chung In‐Sik , Han Ki‐Hwan , Park Jae Myung 
Published: March 2010

AbstractAlthough cannabinoids are associated with antineoplastic activity in a number of cancer cell types, the effect in gastric cancer cells has not been clarified. In the present study, we investigated the effects of a cannabinoid agonist on gastric cancer cell proliferation and invasion. The cannabinoid agonist WIN 55,212‐2 inhibited the proliferation of human gastric cancer cells in a dose‐dependent manner and that this effect was mediated partially by the CB1 receptor. We also found that WIN 55,212‐2 induced apoptosis and down‐regulation of the phospho‐AKT expression in human gastric cancer cells. Furthermore, WIN 55,212‐2 treatment inhibited the invasion of gastric cancer cells, and down‐regulated the expression of MMP‐2 and VEGF‐A through the cannabinoid receptors. Our results open the possibilities in using cannabinoids as a new gastric cancer therapy. J. Cell. Biochem. 110: 321–332, 2010. © 2010 Wiley‐Liss, Inc.
Arsenite causes down‐regulation of Akt and c‐Fos, cell cycle dysfunction and apoptosis in glutathione‐deficient cells
Authors: Habib Geetha M. 
Published: March 2010

AbstractArsenic is a well‐known environmental toxicant but the mechanism by which it causes cytotoxicity is poorly understood. Arsenite induces apoptosis in glutathione (GSH)‐deficient GCS‐2 cells by causing cell cycle dysfunction and down‐regulating critical signaling pathways. This study was designed to examine the effect of arsenite on redox‐sensitive phosphatidylinositol 3‐kinase (PI3K)/Akt, a signaling pathway involved in cell survival and growth, and transcription factor, activating protein‐1 (AP‐1). Arsenite significantly diminished Akt and c‐Fos levels and caused accelerated degradation of these proteins by ubiquitnation. Arsenite also induced cell cycle arrest and apoptosis. The cell cycle arrest involved the down‐regulation of cyclin A2, cyclin D1, cyclin E, cyclin dependent kinases (CDK) 2, CDK4, and CDK6. Apoptosis involved down‐regulation of anti‐apoptotic proteins Bcl‐2, Bcl‐xL, survivin, and inhibitor of apoptosis protein (IAP) and up‐regulation of pro‐apoptotic protein Bax. Taken together, our results suggest that a possible mechanism of arsenite‐induced toxicity under low/no GSH conditions, is to negatively regulate GCS‐2 cell proliferation by attenuating Akt and AP‐1 by ubiquitination and causing cell cycle dysfunction and apoptosis. J. Cell. Biochem. 110: 363–371, 2010. © 2010 Wiley‐Liss, Inc.
Transcriptional repression in ES cells
Authors: Cheong Clara Y. , Lufkin Thomas 
Published: March 2010

AbstractThe dynamics of embryonic stem cell pluripotency is orchestrated by an interplay of transcriptional and epigenetic regulation in a systematic and modular manner. While the ES cell stage is marked by multiple loci with bivalent chromatin marks that prepare genes for imminent activation on differentiation, this open chromatin conformation is tempered by repressive machinery that prevent premature expression of key developmental genes. This review serves to highlight key ES transcription factors and their known links to the epigenetic machinery via known protein complexes. J. Cell. Biochem. 110: 288–293, 2010. © 2010 Wiley‐Liss, Inc.
g_membed: Efficient insertion of a membrane protein into an equilibrated lipid bilayer with minimal perturbation
Authors: Wolf Maarten G. , Hoefling Martin , Aponte‐Santamaría Camilo , Grubmüller Helmut , Groenhof Gerrit 
Published: March 2010

AbstractTo efficiently insert a protein into an equilibrated and fully hydrated membrane with minimal membrane perturbation we present a computational tool, called g_membed, which is part of the Gromacs suite of programs. The input consists of an equilibrated membrane system, either flat or curved, and a protein structure in the right position and orientation with respect to the lipid bilayer. g_membed first decreases the width of the protein in the xy‐plane and removes all molecules (generally lipids and waters) that overlap with the narrowed protein. Then the protein is grown back to its full size in a short molecular dynamics simulation (typically 1000 steps), thereby pushing the lipids away to optimally accommodate the protein in the membrane. After embedding the protein in the membrane, both the lipid properties and the hydration layer are still close to equilibrium. Thus, only a short equilibration run (less then 1 ns in the cases tested) is required to re‐equilibrate the membrane. Its simplicity makes g_membed very practical for use in scripting and high‐throughput molecular dynamics simulations. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
Structural fluctuation and concerted motions in F1‐ATPase: A molecular dynamics study
Authors: Ito Yuko , Ikeguchi Mitsunori 
Published: March 2010

AbstractF1‐ATPase is an adenosine tri‐phosphate (ATP)‐driven rotary motor enzyme. We investigated the structural fluctuations and concerted motions of subunits in F1‐ATPase using molecular dynamics (MD) simulations. An MD simulation for the α3β3γ complex was carried out for 30 ns. Although large fluctuations of the N‐terminal domain observed in simulations of the isolated βE subunit were suppressed in the complex simulation, the magnitude of fluctuations in the C‐terminal domain was clearly different among the three β subunits (βE, βTP, and βDP). Despite fairly similar conformations of the βTP and βDP subunits, the βDP subunit exhibits smaller fluctuations in the C‐terminal domain than the βTP subunit due to their dissimilar interface configurations. Compared with the βTP subunit, the βDP subunit stably interacts with both the adjacent αDP and αE subunits. This sandwiched configuration in the βDP subunit leads to strongly correlated motions between the βDP and adjacent α subunits. The βDP subunit exhibits an extensive network of highly correlated motions with bound ATP and the γ subunit, as well as with the adjacent α subunits, suggesting that the structural changes occurring in the catalytically active βDP subunit can effectively induce movements of the γ subunit. © 2010 Wiley Periodicals, Inc. J Comput Chem 2010
Random number generators tested on quantum Monte Carlo simulations
Authors: Hongo Kenta , Maezono Ryo , Miura Kenichi 
Published: March 2010

AbstractWe have tested and compared several (pseudo) random number generators (RNGs) applied to a practical application, ground state energy calculations of molecules using variational and diffusion Monte Carlo metheds. A new multiple recursive generator with 8th‐order recursion (MRG8) and the Mersenne twister generator (MT19937) are tested and compared with the RANLUX generator with five luxury levels (RANLUX‐[0–4]). Both MRG8 and MT19937 are proven to give the same total energy as that evaluated with RANLUX‐4 (highest luxury level) within the statistical error bars with less computational cost to generate the sequence. We also tested the notorious implementation of linear congruential generator (LCG), RANDU, for comparison. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
Redox‐induced configuration conversion for thioacetamide dimer can function as a molecular switch
Authors: Liu Haiying , Chen Xiaohua , Bu Yuxiang 
Published: March 2010

AbstractThe electronic switching properties of thioacetamide dimer (TAD) were investigated using the nonequilibrium Green's function method combined with density functional theory for design of a novel molecular switch. The H‐bonded TAD can be converted upon hole‐trapping to a three‐electron (3e)‐bonded configuration with a S∴S linkage which could provide a more favorable channel for charge transfer than the before. The redox‐induced configuration conversion between the H‐bonded and the 3e‐bonded TADs could govern the charge migration through the molecular junction with a considerable difference in conduction currents. The calculated I–V characteristic curves of two configurations exhibit a switching behavior with an On‐Off ratio in a range of about 4.3–7.6 within the applied voltages. Clearly, this hypothetical scheme provides a potential way to explore the novel conformation‐dependent molecular switch. © 2010 Wiley Periodicals, Inc. J Comput Chem 2010
Electron localizability indicators ELI–D and ELIA for highly correlated wavefunctions of homonuclear dimers. II. N2, O2, F2, and Ne2
Authors: Bezugly Viktor , Wielgus Pawel , Kohout Miroslav , Wagner Frank R. 
Published: March 2010

AbstractElectron localizability indicators based on the electron pair density ELI–D and ELIA Electron localizability indicators ELI‐D and ELIA based on the electron pair density are studied for the correlated ground‐state wavefunctions of N2, O2, F2, and Ne2 diatomics. Different basis sets and reference spaces are used for the multireference configuration interaction method following the complete active space calculations to investigate the local effect of electron correlation on the extent of electron localizability in position space determined by the two indicators. The results are complemented by calculations of effective bond order, vibrational frequency, and Laplacian of the electron density at the bond midpoint. It turns out that for O2 and F2, the reliable topology of ELI–D is obtained only at the correlated level of theory. © 2010 Wiley Periodicals, Inc. J Comput Chem 2010
Theoretical study of the microhydration of mononuclear and dinuclear uranium(VI) species derived from solvolysis of uranyl nitrate in water
Authors: Ončák Milan , Schröder Detlef , Slavíček Petr 
Published: March 2010

AbstractThe structures and energetics of mononuclear and dinuclear uranium species formed upon speciation of uranyl(VI) nitrate, UO2(NO3)2, in water are investigated by quantum chemistry using density functional theory and the wavefunction‐based methods (MP2, CCSD, CCSD(T)). We provide a discussion of the basic coordination patterns of the various mono‐ and dinuclear uranyl compounds [(UO2)m(X,Y)2m−1(H2O)n]+ (m = 1, 2; n = 0–4) found in a recent mass spectrometric study (Tsierkezos et al., Inorg Chem 2009, 48, 6287). The energetics of the complexation of the uranyl dication to the counterions OH− and NO3− as well as the degradation of the dinuclear species were studied by reference to a test set of 16 representative molecules with the MP2 method and the B3LYP, M06, M06‐HF, and M06‐2X DFT functionals. All DFT functionals provide structures and energetics close to MP2 results, with M06 family being slightly superior to the standard B3LYP functional. © 2010 Wiley Periodicals, Inc. J Comput Chem 2010
Contrast‐enhanced ultrasound in the diagnosis of malignant mesenchymal liver tumors
Authors: Trojan Joerg , Hammerstingl Renate , Engels Knut , Schneider Arne R. , Zeuzem Stefan , Dietrich Christoph F. 
Published: March 2010

AbstractPurpose.Contrast‐enhanced ultrasound can differentiate malignant from benign hepatic tumors, but has not been studied in malignant mesenchymal liver tumors.Methods.We describe the findings of contrast‐enhanced ultrasound in a cohort of five patients with histological‐proven malignant hepatic mesenchymal tumors.Results.The presence of imaging features such as peripheral (nodular) enhancement, chaotic central vascularization, and absence of contrast enhancement in the late phase allowed differentiation from hemangiomas.Conclusions.If these findings are demonstrated inlarge hepatic tumors, then the diagnosis of hemangioma is unlikely and further workup is necessary. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound, 2010
Molecular characterization and gene expression of the channel catfish ferritin H subunit after bacterial infection and iron treatment
Authors: Liu Hong , Takano Tomokazu , Peatman Eric , Abernathy Jason , Wang Shaolin , Sha Zhenxia , Kucuktas Huseyin , Xu De‐hai , Klesius Phillip , Liu Zhanjiang 
Published: March 2010

AbstractFerritins are the major iron storage protein in the cytoplasm of cells, responsible for regulating levels of intracellular iron. Ferritin genes are widely distributed in both prokaryotes and eukaryotes. In mammals, ferritin molecules are composed of heavy‐ (H) and light‐ (L) chain subunits; amphibian genomes contain three ferritin‐type genes (H; middle, M; and L subunits); and teleost genomes to date contain H and M subunits. The objective of this study was to characterize the ferritin H gene in channel catfish (Ictalurus punctatus) to determine its genomic organization and copy numbers, to determine its patterns of tissue expression, and to establish if it is involved in defense responses of catfish after bacterial infection. The catfish ferritin H gene was completely sequenced and characterized, using both mRNA and genomic DNA. Catfish ferritin H gene has a full‐length mRNA sequence of 999 bp, an open reading frame of 534 bp, and 4,704 bp genomic DNA sequence. Catfish ferritin H has a 5 exon and 4 intron genetic organization, containing a long 5′‐untranslated region, which shares high similarity with mammalian and zebrafish genes. Based on phylogenetic analyses, the catfish ferritin H gene is highly conserved throughout evolution. Southern blot analysis suggested that the ferritin H gene has only one copy in the catfish genome. The catfish ferritin H gene was widely expressed in various healthy tissues. The catfish ferritin H gene was significantly up‐regulated in the liver after intraperitoneal injection of iron dextran and coinjection of Edwardsiella ictaluri and iron dextran treatment, suggesting its role in iron metabolism and immunity. J. Exp. Zool. 313A:359–368, 2010. © 2010 Wiley‐Liss, Inc.
Blindness to alternative scenarios in evidence evaluation
Authors: Rassin Eric 
Published: March 2010

AbstractIn order to prevent miscarriages of justice, police, prosecution, and judges must remain open to alternative scenarios in which the suspect is in fact innocent. In recent years, however, several studies have delivered results suggesting that open‐mindedness is not always standard in criminal procedures. For example, Ask and Granhag (2005) found that police officers' estimation of the incriminating power of investigation findings was not affected by knowledge of an alternative suspect. The current first study replicated these findings in a mixed sample of police officers, district attorneys, and judges. In Study 2, this blindness to alternative scenarios did not emerge in a sample of university students. However, the estimation of the incriminating power of the evidence and the willingness to convict the primary suspect were predicted with the participants' confirmation proneness. Copyright © 2010 John Wiley & Sons, Ltd.
Target selection patterns in rape
Authors: Beauregard Eric , Rebocho Maria Francisca , Rossmo D. Kim 
Published: March 2010

AbstractBoth theoretical and empirical studies of decision making in target selection have shown that this process is highly dependent on the physical environment. However, research specifically investigating decision making in sex offenders' target selection is scarce. The aims of the current study were to (1) identify target selection patterns in a mixed sample of 78 Canadian and Portuguese adult rapists, (2) investigate how geographical decision making influences target selection patterns, and (3) test the influence of the type of environment on target selection patterns. The results indicate that Canadian and Portuguese rapists exhibit different target selection patterns but that their geographical decision making is congruent and consistent with the environment within which they operate. Copyright © 2010 John Wiley & Sons, Ltd.
The consistency of inconsistency in serial homicide: patterns of behavioural change across series
Authors: Sorochinski Marina , Salfati C. Gabrielle 
Published: March 2010

AbstractLinkage analysis is a crucial part of the investigative process when faced with a possible series of related offences. Establishing behavioural consistency (i.e., offender's behaviours consistently present across the series) is at the core of linkage. Recent empirical studies have found little evidence of consistency looking at either individual or groups of behaviours in serial homicide. It is argued that behavioural changes are rooted in the changing cognitive strategies that offenders use to reach their ultimate goal (i.e. the commission of multiple homicides). Factors that could account for these changes include learning, situational factors, loss of control, and changes in the offender's fantasy. Patterns of behavioural change have been identified in serial crimes, such as rape. However, no empirical studies have looked at patterns of behavioural change in serial homicide. The present study examined patterns of consistency and change using a combination of thematic and behavioural subgroup approaches that use Multidimensional Scaling. Thematic differentiation indicative of behavioural manifestations of cognitive strategies was found in all three examined subgroups: planning, wounding, and offender–victim interaction, and patterns of change within these subgroups provided support for the above theories. Looking at behavioural patterns rather than individual behaviours, may be a more fruitful way of examining consistency in serial homicide, and could have significant implications for linkage analysis. Copyright © 2010 John Wiley & Sons, Ltd.