bluuebunny/crossref_metadata_2025_split huggingface dataset:
The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection
Authors: Golke A. , Cymerys J. , Słońska A. , Dzieciątkowski T. , Chmielewska A. , Tucholska A. , Bańbura M. 
Published: April 2012

The xCELLigence system for real-time and label-free analysis of neuronal and dermal cell response to Equine Herpesvirus type 1 infection
Real-time cell electronic sensing (RT-CES) based on impedance measurements is an emerging technology for analyzing the status of cells in vitro. It allows label-free, real time monitoring of the biological status of cells. The present study was designed to assess dynamic data on the cell processes during equine herpesvirus type 1 (EHV-1) infection of ED (equine dermal) cells and primary murine neuronal cell culture. We have demonstrated that the xCELLigence system with dynamic monitoring can be used as a rapid diagnostic tool both to analyze cellular behavior and to investigate the effect of viral infection.
The viability of the genetically diverse C. jejuni and C. coli strains in the macrophage J774 cell line
Authors: Krutkiewicz A. , Klimuszko D. 
Published: April 2012

The viability of the genetically diverse C. jejuni and C. coli strains in the macrophage J774 cell line
The intracellular survival of Campylobacter has been described within epithelial cells as well as in macrophages in vitro. The goal of this study was to estimate the viability of the genetically diverse C. jejuni and C. coli strains in the macrophage J774 cell line. Strains selected for analysis differed with regard to the occurrence of genes encoding specific virulence factors. The present work indicates that was no correlation between the source of isolates and relative intracellular survival.
Detection and identification of banned Processed Animal Protein in feedingstuffs by microscopic and PCR methods
Authors: Weiner A. , Gołębiowska A. , Paprocka I. , Kwiatek K. 
Published: April 2012

Detection and identification of banned Processed Animal Protein in feedingstuffs by microscopic and PCR methods
The aim of the study was to present the results of comparative evaluation of the usefulness of PCR and microscopic methods for the detection of Processed Animal Protein (PAP) in feedingstuffs. In the validation study, the limit of the detection for PCR was determined on 0.05% for beef, 0.1% for pork and 0.2% for poultry meat and bone meal (MBM). Among 62 doubtful samples of feedingstuffs examined by microscopic method 41 (66.13%) were found as positive. Based on the results obtained with the use of the microscopic and PCR methods it is possible to state that the molecular biology methods can, at present, be used as a supplementary method in PAP detection. Key worlds: animal feedingstuffs, meat and bone meal, PCR, PAP
The role of metal ions in biological oxidation - the past and the present
Authors: Kleczkowski M. , Garncarz M. 
Published: April 2012

The role of metal ions in biological oxidation - the past and the presentTwo theories, one based on the metabolism of inorganic substances, the other on metabolism of organic substances, have played an important role in the explanation of the origin of life. They demonstrate that the original environment of life on Earth was seawater containing micronutrients with structural, metabolic and catalytic activity. It is assumed that the first primitive organisms lived around 3.8 billion years ago and it was also then that the first catalytic reaction involving metal ions occurred. Biological oxidation leading to oxidative stress and cell damage in animals represents one of these types of reactions which are responsible for many animal diseases. The role of prooxidative and antioxidative actions of transition metal ions as well as their neuropathological consequences have therefore been the topic for many research projects. There is hope that metal chelates and antioxidants might prove to be a modern mode of therapy for i.e. neurogenerative diseases. The aim of this review is to show the evolution of scientific knowledge on metal ions, their biological oxidation, and an overview of their role in physiology and in pathological processes.
Pre-glacial sedimentary facies of the Point Thomas Formation (Eocene) at Cytadela, Admiralty Bay, King George Island, West Antarctica
Authors: Mozer Anna 
Published: April 2012

Pre-glacial sedimentary facies of the Point Thomas Formation (Eocene) at Cytadela, Admiralty Bay, King George Island, West Antarctica
New evidence of Eocene preglacial environments has been found on the southern coast of Ezcurra Inlet on King George Island, South Shetland Islands, West Antarctica. Plant remains (trunks, leaves, detritus) and carbonaceous seams and beds occur in sedimentary strata in a 4 km long Cytadela outcrop of the Point Thomas Formation. They are an evidence for the presence and diversity of terrestrial vegetation in the northern Antarctic Peninsula region. The forests were composed mostly of Podocarpaceae-Araucaria-Nothofagus, with an undergrowth of hygrophilous and thermophilous ferns, and grew on volcanic slopes and surrounding lowland areas of King George Island during breaks in volcanic activity. The succession that crops out at Cytadela provides a record of changing climatic conditions from a warm and wet climate with extensive vegetation to a much drier climate with limited vegetation and ubiquitous weathering of volcanic bedrock. The geochemical indices of weathering (CIA, PIA and CIW) have narrow and relatively high value ranges (76-88), suggesting moderate to high chemical weathering under warm and humid climate conditions. The decrease in humidity and the decline in plant life through the succession can be related to the gradually cooling climate preceding development of the Oligocene ice cover across the Antarctic continent.
Application of Robust Estimation Methods for the Analysis of Outlier Measurements / Aplikácia Robustných Odhadovacích Metód Pri Analýze Odľahlých Meraní
Authors: Gašincová Silvia , Gašinec Juraj , Weiss Gabriel , Labant Slavomír 
Published: April 2012

Abstract
The basis of mathematical analysis of geodetic measurements is the method of least squares (LSM), whose bicentenary we celebrated in 2006. In geodetic practice, we quite often encounter the phenomenon when outlier measurements penetrate into the set of measured data as a result of e.g. the impact of physical environment. That fact led to modifications of LSM that have been increasingly published mainly in foreign literature in recent years. The mentioned alternative estimation methods are e.g. robust estimation methods and methods in linear programming. The aim of the present paper is to compare LSM with the robust estimation methods on an example of a regression line.
Sphingosine Analogue AAL-R Increases TLR7-Mediated Dendritic Cell Responses via p38 and Type I IFN Signaling Pathways
Authors: Seo Young-Jin , Pritzl Curtis J. , Vijayan Madhuvanthi , Blake Celeste R. , McClain Mariah E. , Hahm Bumsuk 
Published: April 2012

Abstract
Sphingosine analogues display immunosuppressive activities and thus have therapeutic potential in the treatment of autoimmune diseases. In this study, we investigated the effects of the sphingosine analogue AAL-R (FTY720 derivative) on dendritic cell (DC) response upon TLR stimulation. Unlike its known immunosuppressive activity, AAL-R increased TLR7-mediated DC responses by elevating the levels of MHC class I and costimulatory molecules and type I IFN expression and by enhancing the capacity of DCs to induce CD8+ T cell proliferation. Importantly, the stimulatory activity of AAL-R was dependent on type I IFN signaling, as type I IFN receptor-deficient DCs failed to respond to AAL-R. Also, AAL-R activated p38 MAPK to increase type I IFN synthesis and TLR7-mediated DC maturation. These findings enhance our understanding of sphingosine regulation of the host immune system, in particular upon pathogenic infections.
NLRC5 Controls Basal MHC Class I Gene Expression in an MHC Enhanceosome-Dependent Manner
Authors: Neerincx Andreas , Rodriguez Galaxia M. , Steimle Viktor , Kufer Thomas A. 
Published: April 2012

Abstract
Nucleotide-binding domain and leucine-rich repeat (NLR) proteins play important roles in innate immune responses as pattern-recognition receptors. Although most NLR proteins act in cell autonomous immune pathways, some do not function as classical pattern-recognition receptors. One such NLR protein is the MHC class II transactivator, the master regulator of MHC class II gene transcription. In this article, we report that human NLRC5, which we recently showed to be involved in viral-mediated type I IFN responses, shuttles to the nucleus and activates MHC class I gene expression. Knockdown of NLRC5 in different human cell lines and primary dermal fibroblasts leads to reduced MHC class I expression, whereas introduction of NLRC5 into cell types with very low expression of MHC class I augments MHC class I expression to levels comparable to those found in lymphocytes. Expression of NLRC5 positively correlates with MHC class I expression in human tissues. Functionally, we show that both the N-terminal effector domain of NLRC5 and its C-terminal leucine-rich repeat domain are needed for activation of MHC class I expression. Moreover, nuclear shuttling and function depend on a functional Walker A motif. Finally, we identified a promoter sequence in the MHC class I promoter, the X1 box, to be involved in NLRC5-mediated MHC class I gene activation. Taken together, this suggested that NLRC5 acts in a manner similar to class II transactivator to drive MHC expression and revealed NLRC5 as an important regulator of basal MHC class I expression.
NLRC5 Cooperates with the RFX Transcription Factor Complex To Induce MHC Class I Gene Expression
Authors: Meissner Torsten B. , Liu Yuen-Joyce , Lee Kyoung-Hee , Li Amy , Biswas Amlan , van Eggermond Marja C. J. A. , van den Elsen Peter J. , Kobayashi Koichi S. 
Published: April 2012

Abstract
Tight regulation of MHC class I gene expression is critical for CD8 T cell activation and host adaptive-immune responses. The promoters of MHC class I genes contain a well-conserved core module, the W/S-X-Y motif, which assembles a nucleoprotein complex termed MHC enhanceosome. A member of the nucleotide-binding domain, leucine-rich repeat (NLR) protein family, NLRC5, is a newly identified transcriptional regulator of MHC class I genes. NLRC5 associates with and transactivates the proximal promoters of MHC class I genes, although the molecular mechanism of transactivation has not been understood. In this article, we show that NLRC5-mediated MHC class I gene induction requires the W/S and X1, X2 cis-regulatory elements. The transcription factors RFX5, RFXAP, and RFXANK/B, which compose the RFX protein complex and associate with the X1 box, cooperate with NLRC5 for MHC class I expression. Coimmunoprecipitation experiments revealed that NLRC5 specifically interacts with the RFX subunit RFXANK/B via its ankyrin repeats. In addition, we show that NLRC5 can cooperate with ATF1 and the transcriptional coactivators CBP/p300 and general control nonderepressible 5, which display histone acetyltransferase activity. Taken together, our data suggest that NLRC5 participates in an MHC class I-specific enhanceosome, which assembles on the conserved W/S-X-Y core module of the MHC class I proximal promoters, including the RFX factor components and CREB/ATF1 family transcription factors, to promote MHC class I gene expression.
Myeloid Cell-Derived Hypoxia-Inducible Factor Attenuates Inflammation in Unilateral Ureteral Obstruction-Induced Kidney Injury
Authors: Kobayashi Hanako , Gilbert Victoria , Liu Qingdu , Kapitsinou Pinelopi P. , Unger Travis L. , Rha Jennifer , Rivella Stefano , Schlöndorff Detlef , Haase Volker H. 
Published: April 2012

Abstract
Renal fibrosis and inflammation are associated with hypoxia, and tissue pO2 plays a central role in modulating the progression of chronic kidney disease. Key mediators of cellular adaptation to hypoxia are hypoxia-inducible factor (HIF)-1 and -2. In the kidney, they are expressed in a cell type-specific manner; to what degree activation of each homolog modulates renal fibrogenesis and inflammation has not been established. To address this issue, we used Cre-loxP recombination to activate or to delete both Hif-1 and Hif-2 either globally or cell type specifically in myeloid cells. Global activation of Hif suppressed inflammation and fibrogenesis in mice subjected to unilateral ureteral obstruction, whereas activation of Hif in myeloid cells suppressed inflammation only. Suppression of inflammatory cell infiltration was associated with downregulation of CC chemokine receptors in renal macrophages. Conversely, global deletion or myeloid-specific inactivation of Hif promoted inflammation. Furthermore, prolonged hypoxia suppressed the expression of multiple inflammatory molecules in noninjured kidneys. Collectively, we provide experimental evidence that hypoxia and/or myeloid cell-specific HIF activation attenuates renal inflammation associated with chronic kidney injury.
Selective Blockade of Herpesvirus Entry Mediator–B and T Lymphocyte Attenuator Pathway Ameliorates Acute Graft-versus-Host Reaction
Authors: del Rio Maria-Luisa , Jones Nick D. , Buhler Leo , Norris Paula , Shintani Yasushi , Ware Carl F. , Rodriguez-Barbosa Jose-Ignacio 
Published: April 2012

Abstract
The cosignaling network mediated by the herpesvirus entry mediator (HVEM; TNFRSF14) functions as a dual directional system that involves proinflammatory ligand, lymphotoxin that exhibits inducible expression and competes with HSV glycoprotein D for HVEM, a receptor expressed by T lymphocytes (LIGHT; TNFSF14), and the inhibitory Ig family member B and T lymphocyte attenuator (BTLA). To dissect the differential contributions of HVEM/BTLA and HVEM/LIGHT interactions, topographically-specific, competitive, and nonblocking anti-HVEM Abs that inhibit BTLA binding, but not LIGHT, were developed. We demonstrate that a BTLA-specific competitor attenuated the course of acute graft-versus-host reaction in a murine F1 transfer semiallogeneic model. Selective HVEM/BTLA blockade did not inhibit donor T cell infiltration into graft-versus-host reaction target organs, but decreased the functional activity of the alloreactive T cells. These results highlight the critical role of HVEM/BTLA pathway in the control of the allogeneic immune response and identify a new therapeutic target for transplantation and autoimmune diseases.
Defective Autoimmune Regulator-Dependent Central Tolerance to Myelin Protein Zero Is Linked to Autoimmune Peripheral Neuropathy
Authors: Su Maureen A. , Davini Dan , Cheng Philip , Giang Karen , Fan Una , DeVoss Jason J. , Johannes Kellsey P. A. , Taylor Lorelei , Shum Anthony K. , Valenzise Mariella , Meloni Antonella , Bour-Jordan Helene , Anderson Mark S. 
Published: April 2012

Abstract
Chronic inflammatory demyelinating polyneuropathy is a debilitating autoimmune disease characterized by peripheral nerve demyelination and dysfunction. How the autoimmune response is initiated, identity of provoking Ags, and pathogenic effector mechanisms are not well defined. The autoimmune regulator (Aire) plays a critical role in central tolerance by promoting thymic expression of self-Ags and deletion of self-reactive T cells. In this study, we used mice with hypomorphic Aire function and two patients with Aire mutations to define how Aire deficiency results in spontaneous autoimmune peripheral neuropathy. Autoimmunity against peripheral nerves in both mice and humans targets myelin protein zero, an Ag for which expression is Aire-regulated in the thymus. Consistent with a defect in thymic tolerance, CD4+ T cells are sufficient to transfer disease in mice and produce IFN-γ in infiltrated peripheral nerves. Our findings suggest that defective Aire-mediated central tolerance to myelin protein zero initiates an autoimmune Th1 effector response toward peripheral nerves.
Hydrophobic Electron‐Transport Layer for Efficient Tin‐Based Perovskite Solar Cells
Authors: He Feifei , Li Tianpeng , Shen Tao , Zhao Yinghan , Jin Zuoming , Zhang Zhiguo , Pu Yu , Deng Liangliang , Qin Lang , Zhan Yiqiang , Liu Yunqi , Wang Yang , Liang Jia 
Published: June 2024

AbstractWith excellent biocompatibility, a narrow bandgap, and long thermal carrier lifetime, tin‐based perovskite solar cells (TPSCs) are promising within the solar technology. The fullerene derivative indene‐C60 bisadduct (ICBA) is recognized as the most efficient electron‐transport material for TPSCs, thanks to its suitable band structure. Nevertheless, the limited electron transport capability and susceptibility to moisture penetration of ICBA have hindered the progress of TPSCs. Herein, the study proposes a new hydrophobic electron‐transport layer (ETL) comprising a surface‐anchored non‐fullerene n‐type semiconducting polymer layer, poly (naphthalene diimide‐alt‐dithiophenebezothiadiazole) (PNDI‐BT), in conjunction with ICBA. PNDI‐BT exhibits high electron mobilities, a fitting band structure, robust interaction with Sn‐based perovskites, and exceptional moisture resistance, effectively addressing the shortcomings of ICBA. Consequently, this innovative hydrophobic ETL of PNDI‐BT/ICBA enhances electron transport and protects Sn‐based perovskites from moisture. As a result, the inverted TPSCs with the new hydrophobic ETL achieve an impressive efficiency of 13.90%, surpassing TPSCs with the ICBA layer (12.75%). Moreover, even after 1000 h of storage in ambient atmosphere, the encapsulated TPSC maintains a remarkable 81% of its initial efficiency. This comprehensive study seamlessly integrates the synthesis of non‐fullerene n‐type semiconducting polymer and device fabrication, providing valuable insights into designing cutting‐edge ETL structure for inverted TPSCs.
A Versatile C/Fe3O4/C Self‐Heating Electrode for Universal Application of Alternating Magnetic Fields in Electrocatalytic Hydrogen Production
Authors: Zhan Helong , Jiang Zhenzhen , Luo Xingfang , Huang Yuan , Ye Daojian , Xu Weiyang , Hu Ce , Zhou Hang , Lei Wen , Yuan Cailei 
Published: June 2024

AbstractThe high‐frequency alternating magnetic field (AMF) is considered as a fascinating heating treatment that provides a noninvasive solution to enhance the catalytic efficiency of electrocatalyst. However, practical applications of AMF in electrochemistry are primarily concentrated on magnetic mediums. To broaden its application into nonmagnetic catalysts, herein, a practicable method is reported by modifying the working electrode substrate with magnetic Fe3O4 nanoparticles (NPs), which serves as both substrate and self‐heating medium by virtue of its rapid and efficient magnetic heating effect associated with Néel relaxation triggered by external high‐frequency AMF, thus boosting the catalyst performance upon it. To verify it, Pt NPs and Pt single atoms (SAs), as two representative non‐magnetic catalyst, are selected for trials. The results, reveal that, when AMF is applied, Pt NPs@C/Fe3O4/C and Pt SAs@C/Fe3O4/C display remarkable enhancement of hydrogen evolution reaction magnetocurrent density respectively by ≈ 146% and ≈185%, whereas on unmodified bare glassy carbon there both show unnoticeable change in catalytic performance. The developed strategy opens up a vast space for exploiting the energy of a weak, non‐invasive magnetic field.
Evolution of Naturally Dried MXene‐Based Composite Aerogels with Flash Joule Annealing for Large‐Scale Production of Highly Sensitive Customized Sensors
Authors: Zhu Wenqi , Zhuang Yuhang , Weng Jianqiang , Huang Qinzhui , Lai Guobin , Li Liuyan , Chen Meixin , Xia Kailai , Lu Zhixing , Wu Mingmao , Zou Zhigang 
Published: June 2024

AbstractMXene aerogels, known for good electrical properties, offer immense potential for the development of high‐sensitivity pressure sensors. However, the intrinsic challenges stemming from the poor self‐assembly capability and high hydrophilicity of MXene impede the natural drying process of MXene‐based hydrogels, thereby constraining their application on a large scale in sensor technology. Herein, a graphene‐assisted approach aimed at modulating the hydrophobicity and enhancing framework strength of MXene through a well‐designed prefreezing technique incorporating 3D spherical macroporous structures is proposed. This synergistic strategy enables the fabrication of naturally dried MXene aerogels across various size scales. Moreover, the integration of 3D spherical macroporous structures improves elasticity and electrical responsiveness of aerogels. Consequently, the aerogel sensor exhibits great performances, including high sensitivity (1250 kPa−1), low detection limit (0.4 Pa), wide frequency response range (0.1–8 Hz), and excellent stability (1000 cycles). This sensor proves adept at monitoring pressure signals ranging from lightweight paper to human motion. Additionally, the application of customized laser engraving endows aerogels with unique functionalities, such as compressibility and immunity to strain, stretchability and resistance to compression, as well as wind detection. Thus, the proposed approach holds significant promise as a scalable method for the mass production of aerogels with versatile applications.
Similarities and Differences between Gas Diffusion Layers Used in Proton Exchange Membrane Fuel Cell and Water Electrolysis for Material and Mass Transport
Authors: Zhang Tao , Meng Ling , Chen Chengcheng , Du Lei , Wang Ning , Xing Lixing , Tang Chunmei , Hu Jian , Ye Siyu 
Published: June 2024

AbstractProton‐exchange membrane fuel cells (PEMFCs) and water electrolysis (PEMWE) are rapidly developing hydrogen energy conversion devices. Catalyst layers and membranes have been studied extensively and reviewed. However, few studies have compared gas diffusion layers (GDLs) in PEMWE and PEMFC. This review compares the differences and similarities between the GDLs of PEMWE and PEMFC in terms of their material and mass transport characteristics. First, the GDL materials are selected based on their working conditions. Carbon materials are prone to rapid corrosion because of the high anode potential of PEMWEs. Consequently, metal materials have emerged as the primary choice for GDLs. Second, the mutual counter‐reactions of the two devices result in differences in mass transport limitations. In particular, water flooding and the effects of bubbles are major drawbacks of PEMFCs and PEMWE, respectively; well‐designed structures can solve these problems. Imaging techniques and simulations can provide a better understanding of the effects of materials and structures on mass transfer. Finally, it is anticipated that this review will assist research on GDLs of PEMWE and PEMFC.