bluuebunny/crossref_metadata_2025_split huggingface dataset:
Loneliness in narcissistic vulnerability: Examining domains of personality functioning
Authors: Finch Ellen F. , Kealy David 
Published: April 2024

AbstractResearch has indicated loneliness to be a detrimental correlate of narcissistic vulnerability. However, no research has examined if this relationship persists across time or why it occurs. The present study explores the link between narcissistic vulnerability and loneliness across time and examines which domains of personality dysfunction (i.e., identity, self‐direction, empathy, and intimacy) may account for why narcissistic vulnerability is related to loneliness. Adult participants (N = 298) were recruited from Prolific and completed study questionnaires assessing narcissistic vulnerability, narcissistic grandiosity, loneliness, domains of self and interpersonal functioning, and neuroticism. Two months later, participants repeated the measure of loneliness. Correlations among all study variables were calculated, followed by linear regression analyses to investigate how personality functioning domains may mediate the longitudinal relationship between narcissistic vulnerability and loneliness. The effects of baseline loneliness, relationship status, and trait neuroticism were controlled for. Narcissistic vulnerability, but not narcissistic grandiosity, was associated with loneliness at baseline and 2 months later. Intimacy functioning, but not identity, self‐direction, or empathy functioning, was a significant mediator of the relationship between narcissistic vulnerability and loneliness over time. These findings suggest that narcissistic vulnerability may contribute to the development or maintenance of loneliness across time, highlighting the role of impaired intimacy functioning in this relationship. The impact of these findings on our understanding of interpersonal functioning in narcissistic vulnerability, as well as clinical implications, is discussed.
Synthesis, Characterization, and Evaluation of Antioxidant, Anti‐Inflammatory, and Antimicrobial Activities of Zinc Oxide Nanoparticles Using Adiantum capillus‐veneris L. Leaf Extract
Authors: Shnawa Bushra H. , Jalil Parwin J. , Mhammedsharif Renjbar M. , Faqe Bakhtiyar A. , Ahmed Meysam H. , Ibrahim Hawar N. , Ahmed Mukhtar H. 
Published: April 2024

AbstractDue to its unique properties and advantageous traits, zinc oxide has garnered significant attention in recent years for the green synthesis of ZnO nanoparticles (ZnO‐NP). This paper is focused on the synthesis of ZnO‐NPs mediated by Adiantum capillus‐veneris L. leaf extract and assesses their potential biological activities. In this study, ZnO‐NP is synthesized using A. capillus‐veneris L. leaf extract. The synthesized nanoparticles are characterized using, UV–vis spectroscopy, energy‐dispersive X‐ray spectroscopy (EDX), (SEM) scanning electron microscopy, X‐ray diffraction (XRD), and Fourier transform infrared (FT‐IR) spectrophotometry. The antibacterial, antifungal, anti‐inflammatory, and antioxidant properties of the formulated ZnO‐NPs are also inspected. The UV–vis, XRD, SEM, EDX, and FTIR confirmed the formation and purity of synthesized ZnO‐NPs. The ZnO‐NPs exhibit efficient antimicrobial potency against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Aspergillus niger, and Aspergillus fumigatus. Gram‐positive bacteria, particularly S. aureus, are more susceptible to ZnO‐NPs, with the largest inhibition zone of 32 mm. In contrast, E. coli and P. aeruginosa display smaller inhibition zones of 25 and 27 mm respectively. Anti‐inflammatory tests show that ZnO‐NPs have a significant anti‐inflammatory effect against egg albumin and bovine serum albumin denaturation, possess low toxicity on erythrocytes, and are highly hemocompatible.
P1′ specificity of the S219V/R203G mutant tobacco etch virus protease
Authors: Golda Mária , Hoffka Gyula , Cherry Scott , Tropea Joseph E. , Lountos George T. , Waugh David S. , Wlodawer Alexander , Tőzsér József , Mótyán János András 
Published: April 2024

AbstractProteases that recognize linear amino acid sequences with high specificity became indispensable tools of recombinant protein technology for the removal of various fusion tags. Due to its stringent sequence specificity, the catalytic domain of the nuclear inclusion cysteine protease of tobacco etch virus (TEV PR) is also a widely applied reagent for enzymatic removal of fusion tags. For this reason, efforts have been made to improve its stability and modify its specificity. For example, P1′ autoproteolytic cleavage‐resistant mutant (S219V) TEV PR was found not only to be nearly impervious to self‐inactivation, but also exhibited greater stability and catalytic efficiency than the wild‐type enzyme. An R203G substitution has been reported to further relax the P1′ specificity of the enzyme, however, these results were obtained from crude intracellular assays. Until now, there has been no rigorous comparison of the P1′ specificity of the S219V and S219V/R203G mutants in vitro, under carefully controlled conditions. Here, we compare the P1′ amino acid preferences of these single and double TEV PR mutants. The in vitro analysis was performed by using recombinant protein substrates representing 20 P1′ variants of the consensus TENLYFQ*SGT cleavage site, and synthetic oligopeptide substrates were also applied to study a limited set of the most preferred variants. In addition, the enzyme–substrate interactions were analyzed in silico. The results indicate highly similar P1′ preferences for both enzymes, many side‐chains can be accommodated by the S1′ binding sites, but the kinetic assays revealed lower catalytic efficiency for the S219V/R203G than for the S219V mutant.
Pharmacogenetic variability of tuberculosis biomarkers in native and mestizo Peruvian populations
Authors: Jaramillo‐Valverde Luis , Levano Kelly S. , Tarazona David D. , Capristano Silvia , Sanchez Cesar , Poterico Julio A. , Tarazona‐Santos Eduardo , Guio Heinner 
Published: April 2024

AbstractIn Peru, 29 292 people were diagnosed with tuberculosis in 2022. Although tuberculosis treatments are effective, 3.4%–13% are associated with significant adverse drug reactions, with drug‐induced liver injury (DILI) considered the most predominant. Among the first‐line antituberculosis drugs, isoniazid is the main drug responsible for the appearance of DILI. In liver, isoniazid (INH) is metabolized by N‐acetyltransferase‐2 (NAT2) and cytochrome P450 2E1 (CYP2E1). Limited information exists on genetic risk factors associated with the presence of DILI to antituberculosis drugs in Latin America, and even less is known about these factors in the native and mestizo Peruvian population. The aim of this study was to determine the prevalence of NAT2 and CYP2E1 genotypes in native and mestizo population. An analytical cross‐sectional analysis was performed using genetic data from mestizo population in Lima and native participants from south of Peru. NAT2 metabolizer was determined as fast, intermediate and slow, and CYP2E1 genotypes were classified as c1/c1, c1/c2 and c2/c2, from molecular tests and bioinformatic analyses. Of the 472 participants, 36 and 6 NAT2 haplotypes were identified in the mestizo and native population, respectively. In mestizo population, the most frequent NAT2*5B and NAT2*7B haplotypes were associated with DILI risk; while in natives, NAT2*5G and NAT2*13A haplotypes were associated with decreased risk of DILI. For CYP2E1, c1/c1 and c1/c2 genotypes are the most frequent in natives and mestizos, respectively. The linkage disequilibrium of NAT2 single nucleotide polymorphisms (SNPs) was estimated, detecting a block between all SNPs natives. In addition, a block between rs1801280 and rs1799929 for NAT2 was detected in mestizos. Despite the limitations of a secondary study, it was possible to report associations between NAT2 and CYP2E alleles with Peruvian native and mestizo by prevalence ratios. The results of this study will help the development of new therapeutic strategies for a Tuberculosis efficient control between populations.
Rutin targets AKT to inhibit ferroptosis in ventilator‐induced lung injury
Authors: Chen Shengsong , Li Zhonghao , Xiao Yuhong , Zhou Zhaobin , Zhan Qingyuan , Yu Lingling 
Published: April 2024

AbstractOur previous research confirmed that rutin reduced ventilator‐induced lung injury (VILI) in mice. Ferroptosis has been reported to participate in the pathogenic process of VILI. We will explore whether rutin inhibits ferroptosis to alleviate VILI. A mouse model of VILI was constructed with or without rutin pretreatment to perform a multiomics analysis. Hematoxylin–eosin (HE) staining and transmission electron microscopy were used to evaluate lung injury in VILI mice. Dihydroethidium (DHE) staining and the malondialdehyde (MDA) and superoxide dismutase (SOD) levels were detected. Molecular docking was performed to determine the binding affinity between rutin and ferroptosis‐related proteins. Western blot analysis, real‐time PCR (RT‐PCR) and immunohistochemical (IHC) staining were conducted to detect the expression levels of GPX4, XCT, ACSL4, FTH1, AKT and p‐AKT in lung tissues. Microscale thermophoresis (MST) was used to evaluate the binding between rutin and AKT1. Transcriptomic and proteomic analyses showed that ferroptosis may play a key role in VILI mice. Metabolomic analysis demonstrated that rutin may affect ferroptosis via the AKT pathway. Molecular docking analysis indicated that rutin may regulate the expression of ferroptosis‐related proteins. Moreover, rutin upregulated GPX4 expression and downregulated the expression of XCT, ACSL4 and FTH1 in the lung tissues. Rutin also increased the ratio of p‐AKT/AKT and p‐AKT expression. MST analysis showed that rutin binds to AKT1. Rutin binds to AKT to activate the AKT signaling pathway, contributing to inhibit ferroptosis, thus preventing VILI in mice. Our study elucidated a possible novel strategy of involving the use of rutin for preventing VILI.
Luteolin: A promising multifunctional natural flavonoid for human diseases
Authors: Zhu Mingtao , Sun Yanping , Su Yang , Guan Wei , Wang Yu , Han Jianwei , Wang Shuang , Yang Bingyou , Wang Qiuhong , Kuang Haixue 
Published: April 2024

AbstractNatural products are closely associated with human health. Luteolin (LUT), a flavonoid polyphenolic compound, is widely found in fruits, vegetables, flowers, and herbs. It is noteworthy that LUT exhibits a variety of beneficial pharmacological properties and holds significant potential for clinical applications, particularly in antitumor, anti‐convulsion, diabetes control, anti‐inflammatory, neuroprotection, anti‐oxidation, anti‐cardiovascular, and other aspects. The potential mechanism of action has been partially elucidated, including the mediation of NF‐κB, toll‐like receptor, MAPK, Wnt/β‐catenin, PI3K/Akt, AMPK/mTOR, and Nrf‐2, among others. The review that aimed to comprehensively consolidate essential information on natural sources, pharmacological effects, therapeutic and preventive potential, as well as potential mechanisms of LUT. The objective is to establish a theoretical basis for the continued development and application of LUT.
Lemborexant‐induced interstitial lung disease: A case report
Authors: Nakahara Satoshi , Ishii Yumiko , Egashira Reika , Tsubouchi Kazuya , Kohno Mikihiro , Takenaka Tomoyoshi , Tanaka Kentaro , Okamoto Isamu 
Published: April 2024

AbstractWe report the first case of drug‐induced interstitial lung disease attributed to lemborexant. A 66‐year‐old man reported to our hospital with the acute onset of cough and breathlessness with ground‐glass opacity on radiological examination. Symptoms were identified after taking lemborexant for 2 consecutive days. The patient had undergone lemborexant treatment 2 years prior and had exhibited no symptoms at that time. The drug‐induced lymphocyte stimulation test for lemborexant was positive. He showed rapid improvement upon treatment with steroid. With the rise in prescriptions of lemborexant for insomnia, lemborexant should be considered as a possible cause of drug‐induced interstitial lung disease.
Neoadjuvant nivolumab plus chemotherapy followed by resection for superior sulcus tumour with high PD‐L1 expression: A case report
Authors: Nakaya Takahito , Hirai Yoshimitsu , Akamatsu Hiroaki , Kojima Fumiyoshi , Iguchi Hideto , Fusamoto Aya , Yata Yumi , Nagai Takahiro , Kitahara Daiki , Takakura Toshiaki , Nishimura Yoshiharu , Yamamoto Nobuyuki 
Published: April 2024

AbstractThe standard treatment for resectable non‐small cell lung cancer (NSCLC) located in the superior sulcus is neoadjuvant chemoradiotherapy followed by highly invasive resection. Based on the results of the CheckMate 816 trial, which showed a marked improvement in the efficacy of neoadjuvant chemo‐immunotherapy, we report a case of minimally invasive resection after neoadjuvant nivolumab plus chemotherapy for superior sulcus NSCLC, resulting in a pathologic complete response. The patient was a 76‐year‐old man with a 65‐mm right superior sulcus tumour diagnosed as squamous cell carcinoma with 95% PD‐L1. After two courses of neoadjuvant nivolumab plus chemotherapy, the tumour was completely resected through an 11‐cm right lateral thoracotomy with second rib resection and first rib preservation. No residual tumour cells were observed in the specimen, and the patient had a pathologic complete response. This report represents a new treatment option for superior sulcus tumours.
Treacherous Territory: Temple Fillers and Tissue Necrosis
Authors: Almutairi Rawan , Usmani Saima , Mubarak Sarah , Aldaraji Wael 
Published: April 2024

Abstract
Temporal region of the face is considered the most promising facial region for inducing panfacial effects, playing an increasingly important role in facial contouring and rejuvenation surgeries, which in turn has led to a rapid growth in the demand for aesthetic correction for temporal hollowing. In order to correct this issue and achieve a more youthful appearance, filler injections can be used. Although this procedure is generally safe and is increasingly popular, complications may happen. Complications include visual loss, neurological deficits, embolism, and non-thrombotic pulmonary embolism. These complications are thought to result from injection of filler material into facial arteries and veins. We describe here a patient who developed pain in the parieto-temporal region following hyaluronic acid (HA) injection for temporal augmentation, which was complicated by tissue necrosis and alopecia. This was managed with hyaluronidase injection and aspirin tablets. Patient was followed for 6 months period after which she had complete hair growth and total resolution of skin necrosis without any scar formation. Since complications of HA injection are wide and serious, practitioners should always be aware of potential consequences of such procedures.
Synthesis of CoFe2O4/TiO2/PANI Nanocomposite Photocatalyst, and Examination of Its Decolorization Efficiency on Rhodamine B
Authors: Keleş Güner Eda , Kızıltaş Hakan , Tırpancı Berivan 
Published: April 2024

AbstractThis study aims to reduce the band gap by doping wide band‐gap TiO2 with CoFe2O4 and PANI to improve the photocatalytic efficiency and examine the decolorization kinetic of Rhodamine B (RhB). The characteristics of the nanocomposites were thoroughly assessed through the utilization of SEM‐EDS, TEM, XRD, FTIR, VSM, UV‐Vis, PL, and XPS analyses. The SEM and TEM analyses revealed that CoFe2O4/TiO2/PANI has a homogeneously porous structure. XRD analysis results were found to show good agreement with the standard diffraction data cards. The band structures obtained by FTIR analyses were compatible with literature studies. The band gap energies determined by UV‐Vis analyses were determined as 2.86 eV for CoFe2O4/TiO2/PANI. The decrease of CoFe2O4/TiO2/PANI recombination rate was proven with PL results. The different initial dye concentrations, temperature, and light intensity parameters were used to determine the decolorization kinetics of RhB over CoFe2O4/TiO2/PANI. The photodegradation rates of RhB in reactions catalyzed by PANI, CoFe2O4, commercial‐TiO2, CoFe2O4/TiO2, and CoFe2O4/TiO2/PANI were determined as 7.6 %, 20.7 %, 26.6 %, 32.4 %, and 48.8 %, respectively. According to data obtained from the decolorization of RhB on CoFe2O4/TiO2/PANI nanocomposite, the appropriate kinetic model was determined Network kinetic. The adsorption equilibrium constant (KB) and activation energy (Ea) were calculated as 0.007 and 7.9 kJ/mol, respectively.
Identification of Phytochemical Inhibitors Targeting VEGFA and TGFBR2 of Cervical Cancer: Insights from Virtual Screening, ADMET and DFT Studies
Authors: Juneja Tanzil , Chakraborty Joydeep , Kapadiya Khushal , Kamdar Jignesh H. 
Published: April 2024

AbstractThe vascular endothelial growth factor A (VEGFA) and Transforming Growth Factor Beta type II receptor (TGFBR2) serves as a promising candidate in the treatment of cervical cancer, playing a crucial role in angiogenesis and tumorigenesis. Its involvement in cervical cancer has been well documented. The pursuit of phytochemical inhibitors to counteract the cancer promoting effects of VEGFA and TGFBR2 has gained considerable momentum as very few drugs are available. In this study, we employed computational techniques to identify potential VEGFA and TGFBR2 inhibitors from 101 phytochemicals. The Spirosolane, Withaferin A & Silybin B for VEGFA and Ginkgetin, Hesperedin for TGFBR2 were identified as promising candidates as indicated by their favorable docking scores and robust energy profiles. Furthermore, Absorption, Distribution, Metabolism, Excretion, and Toxicity (ADMET) profile indicating that Spirosolane and Ginkgetin exhibit favorable drug‐like properties and medicinal chemistry characteristics, suggesting their potential as novel drug candidates. The Density Functional Theory (DFT) studies revealed that these compounds exhibited the highest electrophilicity index for occupied molecular orbitals, basicity, and dipole moment, all contributing to their stability and strong binding affinity at the active site. To advance this line of research, additional experimental validation is warranted to facilitate the development of highly selective and effective VEGFA and TGFBR2 inhibitors.
Synthesis, Biological and Molecular Docking Studies of New Polysubstituted 2‐Amino‐3‐Cyano‐4H‐Chromene Derivatives
Authors: Sul Ramesh D. , Humbe Omkar Y. , Shelar Amruta V. , Patil Rajendra H. , Khedkar Vijay M. , Kale Bharat B. , Nikam Latesh K. 
Published: April 2024

AbstractA series of new substituted 2‐amino‐3‐cyano‐4H‐chromene derivatives have been synthesised by one pot multicomponent reaction of substituted benzaldehyde, malononitrile and 4‐chloro resorcinol. All the synthesised compounds have been characterised using FT‐IR, 1H NMR, 13C NMR and HR‐MS spectra and screened for bioactivities such as swarming behaviour, biofilm formation, biofilm disruption, urease production in Proteus mirabilis. Whereas swarming inhibition was shown by all synthesized chromene derivatives, biofilm inhibition was shown only by nitrophenyl and fluorophenyl substituted chromene derivatives. Interestingly, urease activity was shown by meta substituted nitrophenyl and fluorophenyl derivatives. Quantitative polymerase chain (qPCR) reaction used to investigate the molecular mechanism of most active chromene derivatives showed prominent down regulations of urease enzyme genes. Molecular docking study against urease supported the observed bioactive effect and provide valuable insights into the binding modes and affinities of these new chromene derivatives. The per‐residue interaction analysis proposed the involvement of non‐bonded (steric and electrostatic) and bonded (hydrogen bonding and pi‐pi stacking) interactions with the active site.
Creation of Organic/Inorganic Hybrids in Two‐Dimensional Organized Films Using Natural Materials and Control of Their Morphology
Authors: Nakada Ryoma , Sugita Shogo , Rumon Rokibul Hasan , Fujimori Atsuhiro 
Published: April 2024

AbstractStarch, an insoluble natural polymer, can be spread into a Langmuir monolayer on the surface of water as amylodextrin by solubilization, where it forms a spherical single‐particle layer at the air–water interface. In this study, we fabricated a two‐dimensional mixed monolayer from soluble starch and organo‐modified montmorillonite and attempted to control its morphology by varying the mixing ratio. The behavior of the organo‐modified montmorillonite was dependent on the spreading solvent and miniaturization was possible using a suitable solvent for spreading insoluble starch. Although both materials formed phase‐separated systems, nearly homogeneous particle layers were formed in some cases because of clay miniaturization. However, X‐ray photoelectron spectroscopy showed that the clay components remained intact and a rectangular morphology derived from aluminosilicate was observed in the mixed film with a high clay ratio. Functionalization as a mixed film clearly demonstrated the hydrophilic properties imparted by the starch particles and the selective adsorption of biomolecules on the clay surface.
A New Strategy for Measuring Salivary Alpha‐Amylase Activity Using Glucometer
Authors: Abdi Farid , Gorgani‐Firuzjaee Sattar , Behroozi Javad , Vahidi Mahmood , Heidari Mohammad Foad 
Published: April 2024

AbstractAlpha‐amylase is one of the most important enzymes in the saliva. It is an important biomarker for many health conditions. Saliva sampling is a non‐invasive method and is the preferred choice for sampling at point of the care and home testing. Measurement of salivary alpha‐amylase activity as a rapid test using test strips with Flavin‐dependent glucose dehydrogenases (FAD‐GDH) as glucose is the aim of the current research. FAD‐GDH is an oxygen‐independent enzyme and has a narrow substrate specificity. For this goal, starches from various sources are tested as a substrate for alpha‐amylase and chose MS‐101 starch as the best substrate, between six different starch types. Under optimal assay conditions, when hydrolyzed starch (in 20 min, 37 °C, pH = 7) reacts with FAD‐GDH enzymes that exist in test strips for 5 s glucometer can show the amount of glucose produced in samples from patient samples. This glucose value is directly correlated with the salivary alpha‐amylase activity (p‐value <0.01).