bluuebunny/crossref_metadata_2025_split huggingface dataset:
Disease-induced changes in bacterial and fungal communities from plant below- and aboveground compartments
Authors: Cao Mingfeng , Huang Songqing , Li Jingjing , Zhang Xiaoming , Zhu Yi , Sun Jingzhao , Zhu Li , Deng Yong , Xu Jianqiang , Zhang Zhihua , Li Qiang , Ai Jixiang , Xie Tian , Li Hengli , Yin Huaqun , Kong Wuyuan , Gu Yabing 
Published: April 2024

Abstract
The plant microbes are an integral part of the host and play fundamental roles in plant growth and health. There is evidence indicating that plants have the ability to attract beneficial microorganisms through their roots in order to defend against pathogens. However, the mechanisms of plant microbial community assembly from below- to aboveground compartments under pathogen infection remain unclear. In this study, we investigated the bacterial and fungal communities in bulk soil, rhizosphere soil, root, stem, and leaf of both healthy and infected (Potato virus Y disease, PVY) plants. The results indicated that bacterial and fungal communities showed different recruitment strategies in plant organs. The number and abundance of shared bacterial ASVs between bulk and rhizosphere soils decreased with ascending migration from below- to aboveground compartments, while the number and abundance of fungal ASVs showed no obvious changes. Field type, plant compartments, and PVY infection all affected the diversity and structures of microbial community, with stronger effects observed in the bacterial community than the fungal community. Furthermore, PVY infection, rhizosphere soil pH, and water content (WC) contributed more to the assembly of the bacterial community than the fungal community. The analysis of microbial networks revealed that the bacterial communities were more sensitive to PVY infection than the fungal communities, as evidenced by the lower network stability of the bacterial community, which was characterized by a higher proportion of positive edges. PVY infection further increased the bacterial network stability and decreased the fungal network stability. These findings advance our understanding of how microbes respond to pathogen infections and provide a rationale and theoretical basis for biocontrol technology in promoting sustainable agriculture.

Key points
• Different recruitment strategies between plant bacterial and fungal communities.
• Bacterial community was more sensitive to PVY infection than fungal community.
• pH and WC drove the microbial community assembly under PVY infection.
Viral entry and translation in brain endothelia provoke influenza-associated encephalopathy
Authors: Kimura-Ohba Shihoko , Kitamura Mieko , Tsukamoto Yusuke , Kogaki Shigetoyo , Sakai Shinsuke , Fushimi Hiroaki , Matsuoka Keiko , Takeuchi Makoto , Itoh Kyoko , Ueda Keiji , Kimura Tomonori 
Published: April 2024

AbstractInfluenza-associated encephalopathy (IAE) is extremely acute in onset, with high lethality and morbidity within a few days, while the direct pathogenesis by influenza virus in this acute phase in the brain is largely unknown. Here we show that influenza virus enters into the cerebral endothelium and thereby induces IAE. Three-weeks-old young mice were inoculated with influenza A virus (IAV). Physical and neurological scores were recorded and temporal-spatial analyses of histopathology and viral studies were performed up to 72 h post inoculation. Histopathological examinations were also performed using IAE human autopsy brains. Viral infection, proliferation and pathogenesis were analyzed in cell lines of endothelium and astrocyte. The effects of anti-influenza viral drugs were tested in the cell lines and animal models. Upon intravenous inoculation of IAV in mice, the mice developed encephalopathy with brain edema and pathological lesions represented by micro bleeding and injured astrocytic process (clasmatodendrosis) within 72 h. Histologically, massive deposits of viral nucleoprotein were observed as early as 24 h post infection in the brain endothelial cells of mouse models and the IAE patients. IAV inoculated endothelial cell lines showed deposition of viral proteins and provoked cell death, while IAV scarcely amplified. Inhibition of viral transcription and translation suppressed the endothelial cell death and the lethality of mouse models. These data suggest that the onset of encephalopathy should be induced by cerebral endothelial infection with IAV. Thus, IAV entry into the endothelium, and transcription and/or translation of viral RNA, but not viral proliferation, should be the key pathogenesis of IAE.
Transtension or transpression? Tectono-metamorphic constraints on the formation of the Monte Grighini dome (Sardinia, Italy) and implications for the Southern European Variscan belt
Authors: Petroccia A. , Carosi R. , Montomoli C. , Iaccarino S. , Forshaw J. B. , Petrelli M. 
Published: April 2024

AbstractThis work presents an integrated structural, kinematic, and petrochronological study of the Monte Grighini dome within the Variscan hinterland–foreland transition zone of Sardinia (Italy). The area is characterised by dextral transpressive deformation partitioned into low- and high-strain zones (Monte Grighini shear zone, MGSZ). Geothermobarometry of one sample of sillimanite-bearing mylonitic metapelite indicates that the Monte Grighini shear zone developed under high-temperature (~ 625 °C) and low-pressure (~ 0.4–0.6 GPa) conditions. In situ U–(Th)–Pb monazite geochronology reveals that the deformation in the shear zone initiated at ca. 315 Ma. Although previous studies have interpreted the Monte Grighini shear zone to have formed in a transtensional regime, our structural and kinematic results integrated with constraints on the relative timing of deformation indicate that it shows similarities with other dextral ductile transpressive shear zones in the Southern European Variscan belt (i.e., the East Variscan Shear Zone, EVSZ). However, dextral transpression in the Monte Grighini shear zone started later than in other portions of the EVSZ within the framework of the Southern European Variscan Belt due to the progressive migration and rejuvenation of deformation from the core to the external sectors of the belt.
Graphical abstract
Biomechanical behavior of all-on-4 concept and alternative designs under different occlusal load configurations for completely edentulous mandible: a 3-D finite element analysis
Authors: Şentürk Ayben , Akaltan Funda 
Published: April 2024

AbstractThe aim of this study was to evaluate the effect of the All-on-4 design and 4 alternative implant-supported fixed prosthesis designs on stress distribution in implants, peri-implant bone, and prosthetic framework in the edentulous mandible under different loading conditions using three-dimensional finite element analysis (3D-FEA).Five different experimental finite element models (Model A (unsplinted 6), Model B (splinted 6), Model C (All-on-4), Model D (axial; 2 anterior, 2 posterior), and Model E (4 interforaminal)) were created. Three different loading conditions were applied (canine loading, unilateral I-loading, and unilateral II-loading). The highest minimum (Pmin) and the maximum (Pmax) principal stress values were acquired for cortical and trabecular bones; the highest von Mises (mvM) stress values were obtained for implants and metal frameworks. Model B and Model D showed the most favorable stress distribution. The All-on-4 design (Model C) also showed acceptable stress values close to those of Model B and Model D in the cortical and trabecular bones. In accordance with the stress values in the bone structure, the lowest stress values were measured in the implants and Co-Cr framework in Model B and Model D. The highest stress values in all structures were measured for unilateral loading- II, while the lowest values were found for canine loading. It was concluded that Model B and Model D experimental models showed better biomechanical performance in all structures. Furthermore, the use of a splinted framework, avoiding cantilevers, results in lower stress transmission. On the other hand, canine loading and unilateral loading-I exhibited the best loading conditions.
The role of aetiology in cardiac manifestations of chronic kidney disease: the CPH-CKD ECHO study
Authors: Christensen Jacob , Landler Nino Emanuel , Olsen Flemming Javier , Sørensen Ida Maria Hjelm , Bjergfelt Sasha Saurbrey , Ballegaard Ellen Linnea Freese , Feldt-Rasmussen Bo , Hansen Ditte , Kamper Anne-Lise , Christoffersen Christina , Bro Susanne , Biering-Sørensen Tor 
Published: April 2024

Abstract
Purpose
We investigated the associations between cardiac parameters and aetiologies of CKD in an exploratory study.

Methods
The study population consisted of 883 participants, 174 controls and 709 patients with aetiologies of CKD including diabetic nephropathy/renovascular KD in diabetes mellitus, hypertensive/renovascular nephropathy, tubulointerstitial nephritis, glomerulonephritis/vasculitis, polycystic KD (PKD), and CKD of unknown origin. Echocardiographic measures included left ventricular (LV) ejection fraction, global longitudinal, area, and radial strain, E/e’ ratio, and LV mass index. These were compared between each aetiological group and controls in unadjusted and adjusted analysis.

Results
In unadjusted analysis, patients with diabetic nephropathy/renovascular KD in diabetes mellitus, had impaired LV ejection fraction (Median [IQR]: 56% [49.9,60.69] vs. 60.8% [57.7,64.1]), global longitudinal (mean ± SD: 13.1 ± 3.5% vs. 15.5 ± 2.6%), area (24.1 ± 5.8% vs. 28.5 ± 4.2%), and radial strain (36.2 ± 11.2% vs. 44.1 ± 9.7%), and increased LV mass index (89.1 g/m2 [71.8,104.9] vs. 69,0 g/m2 [57.9,80.8]) and E/e’ ratio (10.6 [8.5,12.6] vs. 7 [5.8,8.3], p < 0.001 for all) compared with controls. Associations were similar for CKD of unknown origin. Patients with hypertensive/renovascular nephropathy had impaired global longitudinal and area strain, and higher E/e’ ratio. Patients with glomerulonephritis/vasculitis had higher LV mass index, while patients with PKD had better global longitudinal strain than controls. All findings remained significant in adjusted analysis, except for the impaired global longitudinal strain in hypertensive/renovascular nephropathy.

Conclusion
Glomerulonephritis/vasculitis, hypertensive/renovascular nephropathy, CKD of unknown origin, and diabetic nephropathy/renovascular KD in diabetes mellitus were increasingly associated with adverse cardiac findings, while PKD and tubulointerstitial nephritis were not. Aetiology might play a role regarding the cardiac manifestations of CKD.

Graphical Abstract
A graphical summary of the study population and main results. Abbreviations: DN = Diabetic nephropathy and renovascular kidney disease in diabetes mellitus, PKD = Polycystic kidney disease, CKDu = Chronic kidney disease of unknown origin, LVEF = Left ventricular ejection fraction, LVMi = Left ventricular mass index, E/e’ ratio = Early mitral inflow velocity to mitral annular early diastolic velocity ratio, GLS = Global longitudinal strain, GAS = Global area strain, GRS = Global radial strain.
Trusting Intelligent Automation in Expert Work: Accounting Practitioners’ Experiences and Perceptions
Authors: Ala-Luopa Saara , Olsson Thomas , Väänänen Kaisa , Hartikainen Maria , Makkonen Jouko 
Published: April 2024

Abstract
AI-based applications are increasingly used in knowledge-intensive expert work, which has led to a discussion regarding their trustworthiness, i.e., to which degree these applications are ethical and reliable. While trust in technology is an important aspect of using and accepting novel information systems, little is known about domain experts’ trust in machine learning systems in their work. To provide a real-life, empirical perspective on the topic, this study reports findings from an interview study of accounting practitioners’ (N = 9) trust in intelligent automation in their work. The findings underline the holistic nature of trust, suggesting that contextual and social aspects, such as participatory design practices, shape domain experts’ trust in intelligent automation. For instance, the participants emphasize their contribution to product development and open communication with the system developers. In addition, the findings shed light on the characteristics of domain experts as technology users, such as the necessity of situation-specific expert knowledge when evaluating the systems’ reliability. Thus, our findings suggest that trust in intelligent automation manifests at different levels, both in human-AI interaction and interpersonal communication and collaboration. This research contributes to the existing literature on trust in technology, especially AI-powered applications, by providing insights into trust in intelligent automation in expert work.
Fracture toughness of AlSi10Mg alloy produced by LPBF: effects of orientation and heat treatment
Authors: Lupi Giorgia , Minerva Giuliano , Patriarca Luca , Casati Riccardo , Beretta Stefano 
Published: April 2024

AbstractIn this study, AlSi10Mg samples were manufactured by laser-powder bed fusion process to explore the fracture toughness dependence on both built orientation and aging treatment. The experiments were performed on as-built and directly-aged (200$$^{\circ }$$

∘

C/4 h) conditions, with the latter revealed to be a valuable treatment for improving fracture toughness. A comprehensive investigation involving detailed microstructural analysis, grain-orientation mapping, and crack-tip strain measurements was conducted to investigate the mechanisms governing the material behavior. The results revealed that specimens subjected to direct aging display higher toughness, thereby enhancing the fracture resistance of AlSi10Mg. Moreover, a considerable variation in fracture toughness values was observed for the different printing orientations, indicating the existence of manufacturing-induced anisotropy. The findings highlight that this anisotropy mainly correlates with the distinctive microstructure induced by the additive manufacturing process. In particular, this study focuses on the different preferential crack paths dictated by the melt pool boundaries orientation respective the crack propagation direction. A substantial reduction in fracture toughness was observed when the crack propagates along the melt pool boundaries.
Optoelectronic properties of BiCuOSe p-type oxychalcogenides
Authors: Melchor-Robles J. A. , Martínez-Reyes Jacobo , Lira Miguel Ángel Meléndez , de Moure Flores Francisco Javier , Olvera M. de la L. 
Published: April 2024

AbstractBiCuOSe systems are proposed as candidates to develop transparent p-type semiconductors in the visible region. This work reports the characterization results obtained for the BiCuOSe powders, synthesized by the solid-state reaction (SSR) method through mechanical milling, and nanostructured thin films deposited from the same processed powders using the pulsed laser deposition (PLD) technique. Structural characterization through X-ray diffraction (XRD) showed that the material presents a tetragonal structure with an average crystallite size of 21 nm and a preferential orientation in the (1 0 2) plane. The morphological and particle size evolution of BiCuOSe powders is presented as a function of the milling time. Transmission and scanning electron microscopies confirmed the spherical geometry of the particles in the powders and nanosheets like structure for the films. Particle sizes were also estimated, ranging from 10 to 100 nm for powders and 60 to 70 nm for films. The bandgap values, EG, for BiCuOSe powders were estimated from diffuse reflectance spectra using the Kubelka–Munk method, yielding values close to 0.7 eV. For thin films, EG values were estimated using the Tauc method, obtaining values in the range of 0.8–3.5 eV, depending on the annealing treatment. Additionally, electrical properties were measured in all deposited thin films, confirming the p-type conductivity, a minimal resistivity of 0.0735 Ω cm, hole mobility on the order of 88 cm2/Vs, and carrier concentration of 9.7 × 1018 cm−3.
Whole-Genome Sequencing-Based Confirmatory Methods on RT-qPCR Results for the Detection of Foodborne Viruses in Frozen Berries
Authors: Yang Zhihui , Kulka Michael , Yang Qianru , Papafragkou Efstathia , Yu Christine , Wales Samantha Q. , Ngo Diana , Chen Haifeng 
Published: April 2024

AbstractAccurate detection, identification, and subsequent confirmation of pathogens causing foodborne illness are essential for the prevention and investigation of foodborne outbreaks. This is particularly true when the causative agent is an enteric virus that has a very low infectious dose and is likely to be present at or near the limit of detection. In this study, whole-genome sequencing (WGS) was combined with either of two non-targeted pre-amplification methods (SPIA and SISPA) to investigate their utility as a confirmatory method for RT-qPCR positive results of foods contaminated with enteric viruses. Frozen berries (raspberries, strawberries, and blackberries) were chosen as the food matrix of interest due to their association with numerous outbreaks of foodborne illness. The hepatitis A virus (HAV) and human norovirus (HuNoV) were used as the contaminating agents. The non-targeted WGS strategy employed in this study could detect and confirm HuNoV and HAV at genomic copy numbers in the single digit range, and in a few cases, identified viruses present in samples that had been found negative by RT-qPCR analyses. However, some RT-qPCR-positive samples could not be confirmed using the WGS method, and in cases with very high Ct values, only a few viral reads and short sequences were recovered from the samples. WGS techniques show great potential for confirmation and identification of virally contaminated food items. The approaches described here should be further optimized for routine application to confirm the viral contamination in berries.
Gekommen um zu bleiben: Was kann aus den Pandemieerfahrungen für die Auswirkungen und die positive Gestaltung des Arbeitens im Homeoffice gelernt werden?
Authors: Gerich Joachim 
Published: April 2024

Zusammenfassung
Forschungsresultate zu Auswirkungen des Arbeitens im Homeoffice zeigen überwiegend positive Effekte für Beschäftigte. Dennoch muss in Abhängigkeit organisationaler und personaler Randbedingungen auch mit ungünstigen Effekten gerechnet werden. Die vorliegende Studie untersucht individuelle Effekte des Arbeitens im Homeoffice bei einer Stichprobe von 743 Beschäftigten mittels Befragungsdaten, welche im Zuge der COVID-19 Pandemie erhoben wurden. Die Ergebnisse zeigen, dass vor allem eine „kompensatorische“ Nutzungspraxis, bei der das Homeoffice für zusätzliche Arbeiten im Anschluss an reguläre Arbeit im Betrieb oder an Wochenenden und freien Tagen genutzt wird, mit ungünstigen Effekten wie erhöhtem Technostress, Work-Family Konflikten, geringerem psychologischem Detachment und einer erhöhten Neigung zu Präsentismus assoziiert ist. Diese Nutzungspraxis wird häufiger in Betrieben mit starker Wettbewerbsorientierung, indirekten Steuerungsformen und gering formalisierten Homeoffice-Policies angewandt. Beschäftigte mit geringer Segmentationspräferenz und hohem Bedürfnis nach Autonomie können zwar grundsätzlich stärker von positiven Effekten im Homeoffice profitieren. Durch zugleich häufigere kompensatorische Nutzungspraxis werden die positiven Effekte durch ungünstige Auswirkungen dieser Nutzungsform jedoch reduziert.

Praktische Relevanz: Durch geeignete organisatorische Rahmenbedingungen sollte eine kompensatorische Nutzung von Homeoffice verhindert werden, um negative Effekte auf die Befindlichkeit und Vereinbarkeiten zu vermeiden.
Effect of zeolite and bamboo biochar as CO2 absorbant in concrete
Authors: Revathi Srinivasan , Alice Elizabeth Tania Dobson , Ancy Shadin Sutharson , Keerthana Jegatheesan 
Published: April 2024

Abstract Carbon dioxide (CO2) is one of the major air pollutants that enter the atmosphere. There is a large release of carbon dioxide into the atmosphere as a result of burning fossil fuels in the cement manufacturing industries and many other industries, as well as emissions from gridlock. This increase in CO2 concentration in the atmosphere leads to various ill effects and global warming. To reduce the CO2 level in the atmosphere, efforts were made to prepare concrete that can absorb CO2 by addition of zeolite and bamboo biochar. These materials were chosen because zeolite and bamboo biochar have large pore volume and large specific surface area and so they can absorb more CO2. Zeolite is having more oxygen content and bamboo biochar is having more carbon content which helps in CO2 absorption. In this work, Zeolite is substituted for fine aggregate in the varying ratios of 25% and 50% and bamboo biochar is substituted for cement in the ratios of 0.5%, 1% and 1.5%. The strength properties and CO2 absorbing capacities of various zeolite and bamboo biochar concrete ratios were compared and it was found that concrete with 50% zeolite and 1% bamboo biochar (ZB5) was the optimal mix. The optimal mix was found based on compressive strength, split tensile strength, water absorption, impact strength, amount of CO2 absorption and depth of CO2 penetration in concrete. This optimal mix has a compressive strength of 38.49 MPa which is 7.48% higher than conventional concrete and also has a split tensile strength of 4.39 MPa which is 15% higher than conventional concrete. It was also found that the optimal mix absorbed 1.2 g of CO2 per day and that the depth of CO2 penetration was 15 mm when the concrete cube was kept in the carbonation chamber for 7 days. This study provided necessary information on the addition of zeolite and bamboo biochar in the concrete which enhances both strength properties and CO2 absorption. This study is important because now-a-days the current CO2 emission in the atmosphere is mainly due to several man-made activities. This ZB concrete provides a solution to reduce the amount of CO2 in the atmosphere and can be used in the concrete pavements, sewer pipelines, parapet walls and the environments with higher CO2 concentration and emission.
Graphical Abstract
The Fractional MacroEvolution Model: a simple quantitative scaling macroevolution model
Authors: Lovejoy Shaun , Spiridonov Andrej 
Published: April 2024

AbstractScaling fluctuation analyses of marine animal diversity and extinction and origination rates based on the Paleobiology Database occurrence data have opened new perspectives on macroevolution, supporting the hypothesis that the environment (climate proxies) and life (extinction and origination rates) are scaling over the “megaclimate” biogeological regime (from ≈1 Myr to at least 400 Myr). In the emerging picture, biodiversity is a scaling “crossover” phenomenon being dominated by the environment at short timescales and by life at long timescales with a crossover at ≈40 Myr. These findings provide the empirical basis for constructing the Fractional MacroEvolution Model (FMEM), a simple stochastic model combining destabilizing and stabilizing tendencies in macroevolutionary dynamics, driven by two scaling processes: temperature and turnover rates.Macroevolution models are typically deterministic (albeit sometimes perturbed by random noises) and are based on integer-ordered differential equations. In contrast, the FMEM is stochastic and based on fractional-ordered equations. Stochastic models are natural for systems with large numbers of degrees of freedom, and fractional equations naturally give rise to scaling processes.The basic FMEM drivers are fractional Brownian motions (temperature, T) and fractional Gaussian noises (turnover rates, E+) and the responses (solutions), are fractionally integrated fractional relaxation noises (diversity [D], extinction [E], origination [O], and E− = O − E). We discuss the impulse response (itself representing the model response to a bolide impact) and derive the model's full statistical properties. By numerically solving the model, we verified the mathematical analysis and compared both uniformly and irregularly sampled model outputs with paleobiology series.