bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Nitrogen Fertilization on the Yield and Quality of Field-grown Leaf Lettuce for Spring Harvest
Authors: Chohura Piotr , Kołota Eugeniusz 
Published: January 2010

Effect of Nitrogen Fertilization on the Yield and Quality of Field-grown Leaf Lettuce for Spring Harvest
Two field experiments with leaf lettuce were carried out in 2006-2008. Each of them was set up in a random sub-block design in 3 replications and a two-factorial system. The first factor represented different nitrogen doses that were used to obtain the required N level in the soil prior to planting the seedlings, the levels being: 50, 100 and 150 mg N·dm-3. The second factor incorporated 4 nitrogenous fertilizers which were different in terms of chemical composition, and, more importantly, contained different nitrogen forms for introduction to the soil: ammonium nitrate 34% N-[NH4NO3], calcium nitrate 15.5% N-[Ca(NO3)2xH2O+NH4NO3], ammonium sulphate 20.0% N-[(NH4)2SO4], and ENTEC 26% N-[NH4NO3+(NH4)2SO4+0.8% DMPP]. Two varieties of leaf lettuce were used: Casabella (of the type Lollo Bionda) with green leaves, and Klausia (of the type Lollo Rosa) with red leaves. The best sources of nitrogen for the lettuce plants were the fertilizers ENTEC 26 and ammonium nitrate, which in contrast to calcium nitrate and ammonium sulphate made it possible to obtain significantly higher yields of lettuce. Irrespective of the kind of the nitrogenous fertilizer used, increasing the concentration of nitrogen in the soil from 50 mg N·dm-3 to 100 mg N·dm-3 resulted in a significant increase in the marketable yield of lettuce. At 150 mg N·dm-3 this yield remained at a level similar to that at 100 mg N·dm-3. The lettuce fertilized with ammonium sulphate was marked by the lowest mean nitrate content in the leaves before harvest. The use of gradually higher doses of nitrogen resulted in a reduced dry weight content and lower levels of total and reducing sugars in lettuce leaves. With respect to yield size and quality, the recommended concentration of nitrogen in the soil for leaf lettuce should not exceed 100 mg N·dm-3.
Some Quality Characteristics Including Isothiocyanates Content of Horseradish Cream as Affected by Storage Period
Authors: Kosson Ryszard , Horbowicz Marcin 
Published: January 2010

Some Quality Characteristics Including Isothiocyanates Content of Horseradish Cream as Affected by Storage Period
During two-year experiments the effects of long-term storage at three temperature levels (2°C, 8°C and 18°C) on content of some nutritive components, including pungent compounds - isothiocyanates (ITC), in processed horseradish (cream) were studied. The significant changes of content of reducing sugars, total isothiocyanates and color indexes in horseradish cream after its storage were noted. The higher temperature of storage the higher content of reducing sugar in horseradish cream was observed. Content of allyl isothiocyanate (AITC) and especially phenylethyl isothiocyanate (PEITC) in fresh horseradish cream was lower in comparison to non-processed horseradish roots. Contents of both isothiocyanates decreased significantly during storage period. The highest decline of isothiocyanates level was observed during first four months of storage. The highest temperature of storage studied (18°C) caused faster decline of both isothiocyanates concentration in horseradish cream. To maintain the human health-promoting compounds in processed horseradish, as well as to keep sensory attributes (color), it is suggested to store horseradish cream in cold condition.
The Influence of Postharvest Treatment and Type of Packaging on Quality and Storage Ability of Cauliflower (Brassica oleracea L. var. botrytis)
Authors: Grzegorzewska Maria , Kosson Ryszard 
Published: January 2010

The Influence of Postharvest Treatment and Type of Packaging on Quality and Storage Ability of Cauliflower (Brassica oleracea L. var. botrytis)
The influence of postharvest hot and cold water treatments on the quality and storage ability of cauliflowers were studied. Also the usefulness of different type of unit packaging for cauliflower storage was compared. The cauliflower heads of Fremont F1 cultivar, after different treatments, were stored at 0°C for six weeks. Postharvest hot water treatment with temperature 38-54°C has not influenced on the storage ability of cauliflower. The heads treated with water of temperatures 50°C and 54°C have shown after six weeks of storage higher rooting, greater compactness loss and colour changes than not treated heads. Postharvest cooling of heads with ice water has not affected quality after storage. It was found that the type of packaging influenced on cauliflower quality after storage. The best quality of cauliflowers after 28 and 42 days of storage was maintained in P-Plus film bags and PE film bags with perforation (8 holes ϕ0.04 cm). The satisfactory quality was maintained also in case of heads wrapped in stretch film and stored for 42 days. The unsatisfactory quality due to heads darkening was found for cauliflowers kept in tightly sealed PE film bags.
Review of the Report of the Expert Panel on Older Workers
Authors: Beach Charles M. 
Published: January 2010

This paper offers a general evaluative review of the report of the Expert Panel on Older Workers of July 2008. It is a balanced, clearly written report that emphasizes the importance of individual choice, flexible work arrangements, reduced barriers to employment of older workers, and regional mobility. But the report does not highlight the important gender dimension operating in retirement decisions and in older worker job displacements. The perspective of the report is that of labour economics, but it could have benefited from a more public economics perspective for guiding principles on job displacement issues.
Hydrogen Storage in Metal–Organic Frameworks
Authors: Hu Yun Hang , Zhang Lei 
Published: January 2010

AbstractMetal–organic frameworks (MOFs) are highly attractive materials because of their ultra‐high surface areas, simple preparation approaches, designable structures, and potential applications. In the past several years, MOFs have attracted worldwide attention in the area of hydrogen energy, particularly for hydrogen storage. In this review, the recent progress of hydrogen storage in MOFs is presented. The relationships between hydrogen capacities and structures of MOFs are evaluated, with emphasis on the roles of surface area and pore size. The interaction mechanism between H2 and MOFs is discussed. The challenges to obtain a high hydrogen capacity at ambient temperature are explored.
An in situ Generated Ruthenium Catalyst for the Tishchenko Reaction
Authors: Simon Marc‐Olivier , Darses Sylvain 
Published: January 2010

AbstractA simple and efficient catalytic system for the selective dimerization of aldehydes, the Tishchenko reaction, is described. High yields of symmetrical esters were generally achieved using an in situ generated ruthenium catalyst in association with the electron‐rich and hindered cyclohexyl(diphenyl)phosphane (CyPPh2) ligand.
A flow cytometry‐based assay for measuring invasion of red blood cells by Plasmodium falciparum
Authors: Bei Amy K. , DeSimone Tiffany M. , Badiane Aida S. , Ahouidi Ambroise D. , Dieye Tandakha , Ndiaye Daouda , Sarr Ousmane , Ndir Omar , Mboup Souleymane , Duraisingh Manoj T. 
Published: January 2010

AbstractVariability in the ability of the malaria parasite Plasmodium falciparum to invade human erythrocytes is postulated to be an important determinant of disease severity. Both the parasite multiplication rate and erythrocyte selectivity are important parameters that underlie such variable invasion. We have established a flow cytometry‐based method for simultaneously calculating both the parasitemia and the number of multiply‐infected erythrocytes. Staining with the DNA‐specific dye SYBR Green I allows quantitation of parasite invasion at the ring stage of parasite development. We discuss in vitro and in vivo applications and limitations of this method in relation to the study of parasite invasion. Am. J. Hematol., 2010. © 2010 Wiley‐Liss, Inc.
Sex differences of chondrogenic progenitor cells in late stages of osteoarthritis
Authors: Koelling Sebastian , Miosge Nicolai 
Published: January 2010

AbstractObjectiveOsteoarthritis (OA), a mainly degenerative disease, is known to be multifactorial in origin. Gene expression patterns vary between populations and sexes. Sex hormone receptors have been described in the cartilage tissue of animals and humans. We undertook this study to determine whether the regenerative potential of chondrogenic progenitor cells (CPCs) present in the arthritic tissue during the late stages of human OA might also be subject to sex‐specific differences and influenced by sex steroids.MethodsWe analyzed sex‐specific differences in the regenerative potential of CPCs and the involvement of sex hormones in vitro in cartilage samples from patients with late‐stage knee OA, using electrochemiluminescence immunoassay, microarray analysis, real‐time reverse transcription–polymerase chain reaction, immunohistochemistry, Western blot analysis, fluorescence‐activated cell sorting, and cell culture.ResultsWe detected expression of estrogen and testosterone in the OA synovial fluid as well as CPCs positive for estrogen receptor α (ERα), ERβ, and androgen receptor. Both hormones influenced the expression of all 3 receptor genes as well as the chondrogenic potential of CPCs by regulating gene expression of Sox9, Runx2, type II collagen, and type I collagen. We found regulatory effects on the collagens via Sox9 and Runx2 as well as regulatory effects independent of these transcription factors. These effects were sex‐specific and relied on hormone concentrations.ConclusionPhysiologic concentrations of testosterone in men and premenopausal concentrations of estrogen in women have a positive effect on the chondrogenic potential of CPCs in vitro. Therefore, strategies of hormone replacement in the synovial fluid of women and men might have beneficial effects on the regenerative potential of arthritic cartilage tissue in late stages of human OA.
Effectiveness of rituximab treatment in primary Sjögren's syndrome: A randomized, double‐blind, placebo‐controlled trial
Authors: Meijer J. M. , Meiners P. M. , Vissink A. , Spijkervet F. K. L. , Abdulahad W. , Kamminga N. , Brouwer E. , Kallenberg C. G. M. , Bootsma H. 
Published: January 2010

AbstractObjectiveTo study the efficacy and safety of B cell depletion with rituximab, a chimeric murine/human anti‐CD20 monoclonal antibody, in patients with primary Sjögren's syndrome (SS) in a double‐blind, randomized, placebo‐controlled trial.MethodsPatients with active primary SS, as determined by the revised American–European Consensus Group criteria, and a rate of stimulated whole saliva secretion of ≥0.15 ml/minute were treated with either rituximab (1,000 mg) or placebo infusions on days 1 and 15. Patients were assigned randomly to a treatment group in a ratio of 2:1 (rituximab:placebo). Followup was conducted at 5, 12, 24, 36, and 48 weeks. The primary end point was the stimulated whole saliva flow rate, while secondary end points included functional, laboratory, and subjective variables.ResultsThirty patients with primary SS (29 female) were randomly allocated to a treatment group. The mean ± SD age of the patients receiving rituximab was 43 ± 11 years and the disease duration was 63 ± 50 months, while patients in the placebo group were age 43 ± 17 years and had a disease duration of 67 ± 63 months. In the rituximab group, significant improvements, in terms of the mean change from baseline compared with that in the placebo group, were found for the primary end point of the stimulated whole saliva flow rate (P = 0.038 versus placebo) and also for various laboratory parameters (B cell and rheumatoid factor [RF] levels), subjective parameters (Multidimensional Fatigue Inventory [MFI] scores and visual analog scale [VAS] scores for sicca symptoms), and extraglandular manifestations. Moreover, in comparison with baseline values, rituximab treatment significantly improved the stimulated whole saliva flow rate (P = 0.004) and several other variables (e.g., B cell and RF levels, unstimulated whole saliva flow rate, lacrimal gland function on the lissamine green test, MFI scores, Short Form 36 health survey scores, and VAS scores for sicca symptoms). One patient in the rituximab group developed mild serum sickness–like disease.ConclusionThese results indicate that rituximab is an effective and safe treatment strategy for patients with primary SS.
The impact of awareness and accessibility on expertise retrieval: A multilevel network perspective
Authors: Yuan Y. Connie , Carboni Inga , Ehrlich Kate 
Published: January 2010

AbstractBuilding on the major premises of transactive memory (TM) theory as well as the recent multilevel extension to the original theory, this study examined the influence of perceived social accessibility of expertise providers, technological accessibility, and awareness of expertise distribution on expertise retrieval. Using social network data collected from a large global sales team, the study found that all three variables had significant impact on expertise retrieval at both the dyadic and individual levels. Our study confirmed the conceptual and theoretical value of approaching TM from a multilevel network perspective.
Gefahr Spannungsrisskorrosion – Systematische Untersuchung des Brückenbestands in Mecklenburg‐Vorpommern
Authors: Hampel Torsten , Bösche Thomas , Anker Stefan , Scheerer Silke , Curbach Manfred 
Published: January 2010

AbstractIn diesem Beitrag wird die systematische Untersuchung des Brückenbestands des Landes Mecklenburg‐Vorpommern hinsichtlich seiner Gefährdung durch Spannungsrisskorrosion beschrieben. Das Projekt war in vier Phasen gegliedert. Zunächst wurde ein Konzept erarbeitet, danach aus dem gesamten Brückenbestand die gefährdeten Bauwerke herausgefiltert und in Kategorien bzw. Bauwerksgruppen eingeteilt. 16 besonders gefährdete Brücken wurden genauer untersucht. In der zweiten Phase wurden die Erkenntnisse aus dieser 1. Phase auf den gesamten Bauwerksbestand übertragen und eine Prioritätenliste erstellt. Außerdem fanden Beprobungen erster ausgewählter Bauwerke statt. Auf die Phasen 3 und 4, bei denen weiterführende theoretische Untersuchungen für spezielle Bauwerke im Vordergrund stehen, wird in diesem Beitrag nicht näher eingegangen.Hazard stress corrosion cracking – Systematical analysis of bridges in Mecklenburg‐Vorpommern.In this article, the systematic investigation of all prestressed bridges of Mecklenburg‐Vorpommern with regard to its exposure to stress corrosion cracking is described. The project was divided into four phases. First, a generally concept was developed. Then the endangered buildings were divided into categories. 16 bridges have been investigated. In the second phase, the findings from phase 1 have been transferred to all buildings in the stock and a priority list drawn up. In addition, first bridges were sampled. In the phase 3 and 4 were more theoretical studies and specific buildings in the foreground. These phases are not content of this paper.
Maternal and paternal risk factors for anorectal malformations: A Dutch case‐control study
Authors: van Rooij Iris A. L. M. , Wijers Charlotte H. W. , Rieu Paul N. M. A. , Hendriks Hester S. , Brouwers Marijn M. , Knoers Nine V. , de Blaauw Ivo , Roeleveld Nel 
Published: January 2010

AbstractBACKGROUNDAnorectal malformations (ARM) are major congenital malformations that usually require a multitude of surgical procedures at a very early age and have a large impact on the lives of patients and their parents. The causes of ARM are still largely unknown, but they are assumed to have a multifactorial etiology. A few studies focused on environmental risk factors, but evidence is still scarce.METHODSIn this Dutch case‐control study (1996–2008), we investigated the role of maternal and paternal risk factors in the etiology of ARM. Parents of 85 ARM cases and 650 controls filled in a questionnaire. Controls were children treated with ear ventilation tubes.RESULTSA higher occurrence of fever during the first trimester of pregnancy was found for case mothers compared to control mothers (odds ratio [OR], 5.1; 95% Confidence Interval [CI], 0.9, 28.1). Maternal occupational exposure to industrial cleaning agents and solvents increased the risk of ARM three times (OR, 2.9; 95% CI, 0.9, 9.3). Overweight (Body Mass Index [BMI] ≥ 25 kg/m2) before pregnancy also seemed to be associated with ARM (OR, 1.8; 95% CI, 1.1, 2.8), as well as maternal multivitamin use during pregnancy (OR, 1.6; 95% CI, 1.0, 2.7), paternal smoking (OR, 1.8; 95% CI, 1.1, 2.9), and paternal occupational exposure to exhaust fumes (OR, 1.9; 95% CI, 1.0, 3.6). Reported ARM in at least one first‐ or second‐degree family member greatly increased the risk of having a child with an ARM (OR, 40.3; 95% CI, 4.8, 342.8).CONCLUSIONSThis study revealed potential risk factors for ARM, including fever during pregnancy, maternal overweight, use of multivitamins, paternal smoking, and occupational exposures, but a familial component seems important as well. Birth Defects Research (Part A), 2010. © 2010 Wiley‐Liss, Inc.
Optimization of the expression of recombinant human activin A in the yeast Pichia pastoris
Authors: Fredericks Dale , Clay Robert , Warner Tracy , O'Connor Anne , de Kretser David M. , Hearn Milton T. W. 
Published: January 2010

AbstractWe report a new procedure to express recombinant human activin A using the methanolic yeast, Pichia pastoris. Optimization of culture procedures has involved comprehensive examination of the effects of culture vessel shape, volume of broth in the induction and expression cultures, methanol concentration, culturing temperature, and pH of the expression cultures. After this optimization, as well as modification of the native cleavage sites, a laboratory scale procedure has been established which routinely produced 2–10 mg/L amounts of this vital growth factor in the highly efficient, eukaryotic yeast system. This system avoids the need to produce this protein and similar TGF‐β proteins in mammalian cell lines which, in addition to being costly, produce many native binding partners of these cystine knot proteins, a factor which can dramatically affect yields of the target protein. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Alcohol biosensing by polyamidoamine (PAMAM)/cysteamine/alcohol oxidase‐modified gold electrode
Authors: Akin Mehriban , Yuksel Merve , Geyik Caner , Odaci Dilek , Bluma Arne , Höpfner Tim , Beutel Sascha , Scheper Thomas , Timur Suna 
Published: January 2010

AbstractA highly stable and sensitive amperometric alcohol biosensor was developed by immobilizing alcohol oxidase (AOX) through Polyamidoamine (PAMAM) dendrimers on a cysteamine‐modified gold electrode surface. Ethanol determination is based on the consumption of dissolved oxygen content due to the enzymatic reaction. The decrease in oxygen level was monitored at −0.7 V vs. Ag/AgCl and correlated with ethanol concentration. Optimization of variables affecting the system was performed. The optimized ethanol biosensor showed a wide linearity from 0.025 to 1.0 mM with 100 s response time and detection limit of (LOD) 0.016 mM. In the characterization studies, besides linearity some parameters such as operational and storage stability, reproducibility, repeatability, and substrate specificity were studied in detail. Stability studies showed a good preservation of the bioanalytical properties of the sensor, 67% of its initial sensitivity was kept after 1 month storage at 4°C. The analytical characteristics of the system were also evaluated for alcohol determination in flow injection analysis (FIA) mode. Finally, proposed biosensor was applied for ethanol analysis in various alcoholic beverage as well as offline monitoring of alcohol production through the yeast cultivation. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Investigation of the Mechanism of Colloidal Silicalite‐1 Crystallization by Using DLS, SAXS, and 29Si NMR Spectroscopy
Authors: Aerts Alexander , Haouas Mohamed , Caremans Tom P. , Follens Lana R. A. , van Erp Titus S. , Taulelle Francis , Vermant Jan , Martens Johan A. , Kirschhock Christine E. A. 
Published: January 2010

AbstractColloidal silicalite‐1 zeolite was crystallized from a concentrated clear sol prepared from tetraethylorthosilicate (TEOS) and aqueous tetrapropylammonium hydroxide (TPAOH) solution at 95 °C. The silicate speciation was monitored by using dynamic light scattering (DLS), synchrotron small‐angle X‐ray scattering (SAXS), and quantitative liquid‐state 29Si NMR spectroscopy. The silicon atoms were present in dissolved oligomers, two discrete nanoparticle populations approximately 2 and 6 nm in size, and crystals. On the basis of new insight into the evolution of the different nanoparticle populations and of the silicate connectivity in the nanoparticles, a refined crystallization mechanism was derived. Upon combining the reagents, different types of nanoparticles (ca. 2 nm) are formed. A fraction of these nanoparticles with the least condensed silicate structure does not participate in the crystallization process. After completion of the crystallization, they represent the residual silicon atoms. Nanoparticles with a more condensed silicate network grow until approximately 6 nm and evolve into building blocks for nucleation and growth of the silicalite‐1 crystals. The silicate network connectivity of nanoparticles suitable for nucleation and growth increasingly resembles that of the final zeolite. This new insight into the two classes of nanoparticles will be useful to tune the syntheses of silicalite‐1 for maximum yield.
Combination of Cobalt and Iron Polypyridine Complexes for Improving the Charge Separation and Collection in Ru(terpyridine)2‐Sensitised Solar Cells
Authors: Caramori Stefano , Husson Jérôme , Beley Marc , Bignozzi Carlo A. , Argazzi Roberto , Gros Philippe C. 
Published: January 2010

AbstractMixtures of polypyridine FeII and CoII complexes are used as electron mediators in Ru–thienyltpy‐sensitised solar cells (tpy=terpyridine). The use of the metalorganic redox couples allows for improved charge‐collection efficiency with respect to the classical iodide/iodine couple which, when associated to Ru–tpy2 dyes, usually produces poor performance. The improved charge collection is explained by a combination of effective dye regeneration and decreased recombination with the oxidised electrolyte on the basis of data obtained by transient spectroscopy and photoelectrochemical measurements. The efficiency of the regeneration cascade is also critically dependent upon the ability of the CoII complex to intercept FeIII centres, as clearly indicated by chronocoulometry experiments.
Fragmenting the S100B–p53 Interaction: Combined Virtual/Biophysical Screening Approaches to Identify Ligands
Authors: Agamennone Mariangela , Cesari Lucia , Lalli Daniela , Turlizzi Elisa , Del Conte Rebecca , Turano Paola , Mangani Stefano , Padova Alessandro 
Published: January 2010

AbstractS100B contributes to cell proliferation by binding the C terminus of p53 and inhibiting its tumor suppressor function. The use of multiple computational approaches to screen fragment libraries targeting the human S100B–p53 interaction site is reported. This in silico screening led to the identification of 280 novel prospective ligands. NMR spectroscopic experiments revealed specific binding at the p53 interaction site for a set of these compounds and confirmed their potential for further rational optimization. The X‐ray crystal structure determined for one of the binders revealed key intermolecular interactions, thus paving the way for structure‐based ligand optimization.