bluuebunny/crossref_metadata_2025_split huggingface dataset:
Essential Role of Complement Mannose-Binding Lectin-Associated Serine Proteases-1/3 in the Murine Collagen Antibody-Induced Model of Inflammatory Arthritis
Authors: Banda Nirmal K. , Takahashi Minoru , Levitt Brandt , Glogowska Magdalena , Nicholas Jessica , Takahashi Kazue , Stahl Gregory L. , Fujita Teizo , Arend William P. , Holers V. Michael 
Published: September 2010

Abstract
Gene-targeted mice deficient in the complement mannose-binding lectin-associated serine protease-1 and -3 (MASP1/3−/−) express only the zymogen of factor D (pro-factor D [pro-Df]), a necessary component of the alternative pathway (AP). We used the murine collagen Ab-induced arthritis (CAIA) model, in which the AP is unique among complement pathways in being both necessary and sufficient for disease induction, to determine whether MASP-1/3 are required in vivo for the development of tissue injury. Disease activity scores, complement C3 tissue deposition in the joint, and histopathologic injury scores were markedly decreased in MASP1/3−/− as compared with wild-type (WT) mice. MASP-1 protein was immunochemically localized to synovial cells of knees of WT mice with arthritis. Pro-Df was present in both synovial cells and chondrocytes of knees of WT and MASP1/3−/− mice without arthritis, with increased amounts present in synovial cells of WT mice with CAIA. No conversion of pro-Df to mature Df was detectable in the serum of MASP1/3−/− mice during the evolution of CAIA. C3 activation and deposition as well as C5a generation induced in vitro by adherent anti-type II collagen mAbs were absent using sera from MASP1/3−/− mice under conditions in which only the AP was active. The addition of human Df fully reconstituted in vitro C3 activation and C5a generation using sera from MASP1/3−/− mice. Our studies demonstrate for the first time, to our knowledge, the absolute requirement for the activity of MASP-1 protein in autoimmune-associated inflammatory tissue injury in vivo through activation of the AP of complement by cleavage of pro-Df to mature Df.
CD4+ T Cells Are Not Required for the Induction of Dengue Virus-Specific CD8+ T Cell or Antibody Responses but Contribute to Protection after Vaccination
Authors: Yauch Lauren E. , Prestwood Tyler R. , May Monica M. , Morar Malika M. , Zellweger Raphaël M. , Peters Bjoern , Sette Alessandro , Shresta Sujan 
Published: September 2010

Abstract
The contribution of T cells to the host response to dengue virus (DENV) infection is not well understood. We previously demonstrated a protective role for CD8+ T cells during primary DENV infection using a mouse-passaged DENV strain and IFN-α/βR−/− C57BL/6 mice, which are susceptible to DENV infection. In this study, we examine the role of CD4+ T cells during primary DENV infection. Four I-Ab–restricted epitopes derived from three of the nonstructural DENV proteins were identified. CD4+ T cells expanded and were activated after DENV infection, with peak activation occurring on day 7. The DENV-specific CD4+ T cells expressed intracellular IFN-γ, TNF, IL-2, and CD40L, and killed peptide-pulsed target cells in vivo. Surprisingly, depletion of CD4+ T cells before DENV infection had no effect on viral loads. Consistent with this observation, CD4+ T cell depletion did not affect the DENV-specific IgG or IgM Ab titers or their neutralizing activity, or the DENV-specific CD8+ T cell response. However, immunization with the CD4+ T cell epitopes before infection resulted in significantly lower viral loads. Thus, we conclude that whereas CD4+ T cells are not required for controlling primary DENV infection, their induction by immunization can contribute to viral clearance. These findings suggest inducing anti-DENV CD4+ T cell responses by vaccination may be beneficial.
Murine B Cells Regulate Serum IgE Levels in a CD23-Dependent Manner
Authors: Cheng Laurence E. , Wang Zhi-En , Locksley Richard M. 
Published: September 2010

Abstract
The manifestations of allergic disorders are closely tied to the biologic effects of IgE activation with Ag. In immediate hypersensitivity reactions, IgE effector function requires prior binding to innate immune cells, primarily mast cells and basophils, with the blood acting as a reservoir for unbound IgE. As the severity of allergic disease is proportional to the size of this unbound IgE pool, we hypothesized that cellular mechanisms exist to limit the size and/or enhance the clearance of free IgE molecules. We examined this in mice by engineering a reporter IgE molecule that allowed us to track the fate of IgE molecules in vivo. The absence of FcεRI-expressing cells did not affect serum IgE levels, but B cells regulated serum IgE by controlling the size of the free IgE pool. B cells captured IgE by direct binding to the low-affinity IgE receptor, CD23. These data indicate a mechanism regulating serum IgE and additionally clarify the role of CD23 in this process.
Although IL-6 Trans-Signaling Is Sufficient To Drive Local Immune Responses, Classical IL-6 Signaling Is Obligate for the Induction of T Cell-Mediated Autoimmunity
Authors: Lissilaa Rami , Buatois Vanessa , Magistrelli Giovanni , Williams Anwen S. , Jones Gareth W. , Herren Suzanne , Shang Limin , Malinge Pauline , Guilhot Florence , Chatel Laurence , Hatterer Eric , Jones Simon A. , Kosco-Vilbois Marie H. , Ferlin Walter G. 
Published: September 2010

Abstract
IL-6–mediated T cell-driven immune responses are associated with signaling occurring through the membrane-bound cognate receptor α-chain (mIL-6Rα). Once formed, IL-6–mIL-6Rα complexes induce the homodimerization and subsequent phosphorylation of the ubiquitously expressed signal-transducing protein, gp130. This signaling event is defined as classical IL-6 signaling. However, many inflammatory processes assigned to IL-6 may be mediated via binding a naturally occurring soluble IL-6Rα, which forms an agonistic complex (IL-6/soluble IL-6Rα) capable of evoking responses on a wide range of cell types that lack mIL-6Rα (IL-6 trans-signaling). To dissect the differential contribution of the two IL-6 signaling pathways in cell-mediated inflammatory processes, we pharmaceutically targeted each using two murine models of human arthritis. Whereas intra-articular neutralization of trans-signaling attenuated local inflammatory responses, the classical pathway was found to be obligate and sufficient to induce pathogenic T cells and humoral responses, leading to systemic disease. Our data illustrate that mechanisms occurring in the secondary lymphoid organs underlying arthropathies are mediated via the classical pathway of IL-6 signaling, whereas trans-signaling contributes only at the local site, that is, in the affected tissues.
Effect of the synthesis initiation mode on the structure and properties of sulfadiazine molecularly imprinted polymers
Authors: Zhang Zhiliang , Wang Bing , Li Jian 
Published: September 2010

AbstractIn the presence of imprinting molecules of sulfadiazine, two kinds of molecularly imprinted polymers (MIPs) were synthesized by photoinitiated and thermally initiated polymerizations, respectively. The combination of sulfadiazine and α‐methyl acrylic acid and their composite ratio were studied by differential UV absorption spectrometry. The results show that a combined effect between the functional monomer and the imprinting molecule occurred in the solution. The structure and properties of these synthetic polymers were researched and compared with IR spectroscopy, transmission electron microscopy, and a balanced combination of static experiments. The polymer materials synthesized in these two ways had the same molecular structure, but their morphology was different. Furthermore, through the adsorption isotherm, Scatchard analysis, and evaluation of the imprinting efficiency, we determined that the lower temperature was a more suitable synthesis condition for the polymerization of the MIPs. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Role of the interface in the melt‐rheology properties of linear low‐density polyethylene/low‐density polyethylene blends: Effect of the molecular architecture of the dispersed phase
Authors: Robledo N. , Vega J. F. , Nieto J. , Martínez‐Salazar J. 
Published: September 2010

AbstractWe studied the melt linear viscoelastic and elongational properties of blends consisting of a Ziegler–Natta linear low‐density polyethylene (LLDPE) and different LDPEs. The weight fraction of the LDPE used in the blends was 15%. The linear viscoelastic characterization was performed at different temperatures for all of the blends to determine the thermorheological behavior in the melt state. The blends fulfilled the time–temperature superposition but exhibited a broad linear viscoelastic response, which was further than that expected for miscible blends and even immiscible systems with a sharp interface. A rheological study of the application of the Palierne model revealed that in addition to the droplet shape relaxation, another mechanism was present at lower frequencies. We discuss the results by hypothesizing a strong interaction between the high‐molecular‐weight linear fraction of the LLDPE matrix and a fraction of molecules of the dispersed phase, which formed a thick interface with its own viscoelastic properties. A clear change in this additional mechanism was observed, depending on the dispersed minor‐phase properties, which produced an impact on the processing of the blends, and more precisely, on the values of the melt strength in the melt‐spinning experiments. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Synthesis and thermal behavior of poly(methyl methacrylate)/Maghnia bentonite nanocomposite prepared at room temperature via in situ polymerization initiated by a new Ni(II)α‐benzoinoxime complex
Authors: Ouaad Kamel , Djadoun Saïd , Ferfera‐Harrar Hafida , Sbirrazzuoli Nicolas , Vincent Luc 
Published: September 2010

AbstractPoly(methyl methacrylate) (PMMA) and poly(methyl methacrylate)/clay nanocomposite (PMMA/OBT) were successfully prepared in dioxan at room temperature via in situ radical polymerization initiated by a new Ni(II)α‐ Benzoinoxime complex as a single component in presence of 3% by weight of an organically modified bentonite (OBT) (originated from Maghnia, Algeria) and characterized by FTIR, 1H‐NMR and viscometry. Mainly intercalated and partially exfoliated PMMA/OBT nanocomposite was elaborated and evidenced by X‐Ray diffraction (XRD) and transmission electron microscopy (TEM). The intrinsic viscosity of PMMA/OBT nanocomposite is much higher than the one of pure PMMA prepared under the same conditions. Differential scanning calorimetry (DSC) displayed an increase of 10°C in the glass transition temperature of the elaborated PMMA/OBT nanocomposite relative to the one of pure PMMA. Moreover, the TGA analysis confirms a significant improvement of the thermal stability of PMMA/OBT nanocomposite compared to virgin PMMA: the onset degradation temperature of the nanocomposite, carried out under nitrogen atmosphere, increased by more than 45°C. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Statistical evaluation of non‐profile analyses for the in vitro bioequivalence
Authors: Chiu Shih‐Ting , Tsai Pei‐Ying , Liu Jen‐pei 
Published: September 2010

AbstractFor locally acting drug products such as nasal aerosols and nasal sprays, the 2003 US Food and Drug Administration (FDA) draft guidance suggests that bioequivalence between generic and brand‐name products be established through in vitro tests. In addition, for non‐profile analyses based on spray content uniformity, droplet size distribution, spray pattern, priming, and re‐priming, the draft US FDA guidance recommends that the population bioequivalence (PBE) between generic and innovator's products be demonstrated. However, the linearized criterion recommended in the draft FDA guidance does not take into consideration the variations due to batches, samples, and life stages. Hence, under a two‐stage nested random effects model, we apply the methods of modified large‐sample (MLS) and generalized pivotal quantities (GPQs) to construct the upper 95% confidence limit for in vitro PBE criterion with consideration of variance components as the statistical testing procedures for establishing the in vitro BE. A simulation study was conducted to compare empirical size and empirical power among the three methods. A numerical example illustrates the proposed methods. Copyright © 2010 John Wiley & Sons, Ltd.
Comparison of PARAFAC and PARALIND in modeling three‐way fluorescence data array with special linear dependences in three modes: a case study in 2‐naphthol
Authors: Chen Hao , Zheng Binghui , Song Yonghui 
Published: September 2010

AbstractOwing to excited‐state proton transfer in 2‐naphthol solutions, the fluorescence excitation–emission matrices (EEMs) have factors that are highly dependent in three modes. For the first time such EEMs are used to compare the capability of PARALIND (PARAFAC with linear dependence) and conventional PARAFAC in modeling three‐way EEMs having linearly dependent factors in three modes. Two primary conclusions have been drawn. First, the results indicate that a 3‐factor PARAFAC model fit the data better than two PARALIND models (type 1 and 2) in this case while equally well with a specially PARALIND model (type 3); second, a negative core consistency (CC) in the 3‐factor PARAFAC model is reported but the type 3 PARALIND model reports a nearly 100 CC. This work has demonstrated that a properly constrained PARALIND can fit the very special EEMs of 2‐naphthol. The presence of negative CC associated with a perfect PARAFAC model would imply the presence of very special linear dependences in EEMs, which would be used as an “alarm” for the investigators to interpret the data more carefully when dealing with complicated environmental EEMs in the absence of a priori knowledge. Copyright © 2010 John Wiley & Sons, Ltd.
Investigation in monolayers of double‐chain fluorocarbon amphiphiles Effect of headgroups on molecular packing and interaction between hydrocarbon and fluorocarbon monolayers
Authors: Liang Kang‐Ning , Hui Yong‐Zheng 
Published: September 2010

AbstractThe surface pressure‐area diagrams of double‐chain fluorocarbon amphiphiles with different headgroup compositions show that the amphiphiles arrange almost perpendicularly to the water subphase and the structure of headgroups exerts significant influence on the amphiphile packing. Strong hydrogen bonding and weak electrostatic interaction favor the formation of stable monolayers. Perfluorooctanoic acid (FOA) cannot form monolayer at water/air interface and can only form liquid monolayer in subphase of calcium nitrate solution. Complete phase separation of palmitic acid and a fluorocarbon amphiphile with shorter hydrocarbon spacer group, 1, could be demonstrated in monolayers by using the phase rule of Crisp. The creation of phase‐separated monolayers is possible when the monolayer is composed of a mixture of palmitic acid and a fluorocarbon amphiphile with longer hydrocarbon spacer group, 2. It can be suggested that the miscibility of hydrocarbon amphiphiles with fluorocarbon amphiphiles is determined by the hydrocarbon fraction of fluorocarbon amphiphiles.
The structure and hydrogen bond of tert‐butoxycarbonyl‐D, L‐isoleucyl‐glycyl methyl ester
Authors: Yang Qing‐Chuan , Dai Sheng , Tang You‐Qi 
Published: September 2010

AbstractThe crystal structure of tert‐butoxycarbonyl‐D, L‐isoleucyl‐glycyl methyl ester was determined by the X‐ray method. The space group is P21 with a = 9.115(3), b= 12.075(4), c = 16.258(5) Å, β = 89.84(3) and Z = 4. It was observed that in the peptides which contain IleGly fragment the carbonyl group of Gly residue has less tendency to form hydrogen bond than that of Ile residue. This tendency was supported by the atomic net charges calculated with the quantum mechanics program CNDO/2.
Preparation of new mixed ligand complexes of lanthanide acetylacetonate with dibenzo‐18‐crown‐6 and their IR spectra
Authors: Yao Ke‐Min , Wang Xiao‐Ling 
Published: September 2010

AbstractFourteen new mixed ligand complexes of lanthanide acetylacetonate and dibenzo‐18‐ crown‐6 (DB18C6) were prepared by three different methods. The composition of the complexes was shown to be Ln(acac)· DB18C6 by elemental analysis and X‐ray powder diffraction. Infrared spectra of the complexes were investigated, and the results indicate that their absorption bands in the region of 400—4000 cm−1are complicated and without much difference from each other, but in the far infrared region various absorption bands of Ln—O can be observed and they changes regularly with the atomic number of lanthanides.
The synthesis and crystal structure of a trinuclear molybdenum cluster compound [Mo3(μ‐O)(μ‐S)3(μ(μ‐OAc)2(dtp)2·(Py)]·0.5H2O
Authors: Lu Shao‐Fang , Huang Jian‐Quan , Huang Zi‐Xiang , Zhang Bin , Huang Jin‐Ling 
Published: September 2010

Abstract[Mo3,OS3(dtp)4(H2O)] reacts with NaOAc·3H2O in Py to give the title compound. The crystal data are as follows: [Mo2OS3)(OAc)2(dtp)2·Py]−0.5H,O(dtp = [S3P(OC2H5)2]−, Py = C5H5N); M = 976.64; triclinic; space group P1; a=11.704(5), b=14.169(7), c= 11.688 (5) Å α=109.94(4) β = 91.53(4), γ = 91.93(4)°; V= 1819(1) Å2; Z=2; Dc = 1.78 g·cm−3 λ(Mo Kα) = 0.71069 Å μ=15.15 cm−1; F(000) = 970 T=296 K; final R=0.071 for 1652 reflections with I>3σ(I). In the molecule, the [Mo3OS3] core is surrounded by two bridging OAc groups and two terminal chelate dtp groups attached to the {Mo3} triangle in a symmetric style, and the Py ligand is coordinated to the Mo atom at the apex of {Mo3} triangle with the nitrogen. This novel configuration is obtained for the first time with Mo—N bond length being 2.27 (2) Å and three Mo—Mo bond lengths 2.584 (4), 2.587 (4) and 2.657(4) Å, respectively. As a whole, the molecule has a virtual C2 symmetry.
The reaction of perfluoroalkanesulfonyl halides IX. The syntheses of bisperfluoroalkyl butadienes and perfluoroalkylated dienoates
Authors: Huang Wei‐Yuan , Zhang Han‐Zhong 
Published: September 2010

AbstractThe title compounds were prepared in good yield through the reaction of perfluoroalkyl butadiene and 2,4‐dienoates with perfluoroalkanesulfonyl bromide in the presence of benzoyl peroxide followed by HBr elimination.
The spin delocalization substituent parameter σ˙. 7. Meta‐substituent effects on the cyclodimerization of trifluorostyrenes. A test for the σ˙JJ approach
Authors: Jiang Xi‐Kui , Ji Guo‐Zhen , Zhang Xiu‐Lian , Guo Hong‐Xun 
Published: September 2010

AbstractRate constants (k's) of the thermal cyclodimerization of ten meta‐substituted trifluorostyrenes (m‐Y‐TFS's) have been measured in the temperature range 110–160°C. On the basis of these k's and the σmb values derived from 19F NMR data the spin‐delocalization substituent constant σ˙m values of these ten meta‐substituents have been calculated. The averaged σ˙m values are: m‐Et, 0.09; m‐t‐Bu, 0.11; m‐F, 0.03; m‐Cl,−0.05; m‐Br, 0.12: m‐CF3, −0.07; m‐OMe, 0.10; m‐NO2, −0.002; m‐CN, 0.11 and m‐CO2Me, 0.08. Both the smallness of these values and the nonexistence of correlation between Hammett‐Type polar σX and these σ˙m values support our belief that the σj̇J approach is reliable and trustworthy.
Cyclization of 2,6‐dialkoxy acetophenone hydrazones in the presence of polyphosphoric acid (PPA)
Authors: Qiu Yu‐Zhu , Zhang Zheng , Zhong Zhen‐Qi , Hu Hong‐Wen 
Published: September 2010

AbstractCyclization of a series of 2,6‐dialkoxy acetophenone hydrazones gave 3‐methyl‐4‐alkoxyindazoles with elimination of the 2‐ or 6‐alkoxy group in the presence of PPA. The reaction mechanism was discussed.
Studies on the synthesis of amino acids XIII. Dibenzylation of 2‐phenyl‐2‐oxazolin‐5‐one
Authors: Du Zheng‐Ming , Chen Ji‐Jun , Shi Yao‐Zeng , Hu Hong‐Wen 
Published: September 2010

Abstract4,4‐Dibenzyl‐2‐phenyl‐2‐oxazollin‐5‐ones are prepared by one step reaction of 2‐phenyl‐2‐oxazolin‐5‐one with magnesium methyl carbonate (MMC) and benzylhalides. It is a novel and convenient method for the synthesis of α,α‐disubstituted α‐amino acids by hydrolysis of 4,4‐dibenzyl‐2‐phenyl‐2‐oxazolin‐5‐ones.
Combined Wavelet Transform with Curve‐fitting for Objective Optimization of the Parameters in Fourier Self‐deconvolution
Authors: Zhang Xiu‐Qi , Zheng Jian‐Bin , Gao Hong 
Published: September 2010

AbstractFourier self‐deconvolution was the most effective technique in resolving overlapping bands, in which deconvolution function results in deconvolution and apodization smoothes the magnified noise. Yet, the choice of the original half‐width of each component and breaking point for truncation is often very subjective. In this paper, the method of combined wavelet transform with curve fitting was described with the advantages of an enhancement of signal to noise ratio as well as the improved fitting condition, and was applied to objective optimization of the original half‐widths of components in unresolved bands for Fourier self‐deconvolution. Again, a noise was separated from a noisy signal by wavelet transform, therefore, the breaking point of apodization function can be determined directly in frequency domain. Accordingly, some artifacts in Fourier self‐deconvolution were minimized significantly.
Studies on the Physical Adsorption Equilibria of Gases on Porous Solids over a Wide Temperature Range Spanning the Critical Region—Adsorption on Microporous Activated Carbon
Authors: Zhou Ya‐Ping , Bai Shu‐Pei , Zhou Li , Yang Bin 
Published: September 2010

AbstractAdsorption equilibria of nitrogen and methane on microporous ( < 2 nm) activated carbon were measured for a wide temperature range (103‐298 K) spanning the critical region. Information relating to Henry constants, the isosteric heat of adsorption, and the amount of limiting adsorption were evaluated. All isotherms show type‐I features for both sub‐ and supercritical temperatures. A new isotherm equation and a consideration for the importance of the effect of the adsorbed phase volume allow this kind of isotherms to be modeled satisfactorily. The model parameter of the saturated amount of absolute adsorption (n0t) equals the limiting adsorption amount (nitm), leaving the physical meaning of the latter clarified, and the exponent parameter (q) proves to be an appropriate index of surface heterogeneity.
Synthesis of Diazacrown Ethers with Chromophores and Their Photoinduced Charge‐Separation with Methyl Viologen
Authors: Li Jiu‐Yan , Zhang Li‐Ping , Wu Li‐Zhu , Wang Bo‐Jie , Tung Chen‐Ho 
Published: September 2010

AbstractTwo 4,13‐diaza‐18‐crown‐6 ethers with either two pyrenyl or two carbazolyl groups were synthesized. The two crown ethers can form complexes with methyl viologen in methanol solution. Photoirradiation of the complexes resulted in the electron transfer from the excited states of the chromophores to methyl viologen as demonstrated by the quenching of the chromophore fluorescence and the detection of the absorption spectrum of the generated viologen radical cation. The back electron transfer in these systems was inhibited by the electrostatic repulsion between the positively charged viologen radical cation and the generated chromophore radical cation. Long‐lived charge separation states (up to tens of min) were observed.