bluuebunny/crossref_metadata_2025_split huggingface dataset:
Child‐related determinants of health‐related quality of life in children with brain tumours 1 year after diagnosis
Authors: Penn Anthony , Shortman Robert I. , Lowis Stephen P. , Stevens Michael C.G. , Hunt Linda P. , McCarter Renee J. , Curran Andrew L. , Sharples Peta M. 
Published: October 2010

AbstractObjectivesInfratentorial tumour site and health‐related quality of life (HRQL) 1 month after diagnosis have been shown to predict HRQL 1 year after diagnosis in children with brain tumours. This study aimed to identify additional early child‐related determinants of parent‐ and child‐report HRQL.MethodsLongitudinal prospective study. Semi‐structured interviews took place approximately 1 and 12 months after diagnosis. HRQL was measured using the self‐ and parent‐report Pediatric Quality of Life Scales (PedsQL 4.0) Total Scale Score and Health Utilities Index Mark 3 (HUI3) multi‐attribute utility function. Child variables included performance and verbal IQ, general memory, selective attention executive function, behaviour problems, adaptive behaviour, symptoms of depression and anxiety and event related anxiety. Univariate analyses were used to identify potential early predictors of HRQL. Regression analysis was then used to identify the most important determinants of HRQL at 1 year.ResultsThirty‐five patients completed the 12‐month interviews. Multivariate analysis showed infratentorial tumour site remained an important determinant of HRQL 1 year after diagnosis. Infratentorial tumour site and selective attention at 1 month generally best predicted poor self‐ and parent‐report HRQL at 12 months. Adaptive behaviour and performance IQ may be important.ConclusionSelective attention and infratentorial tumour site are most important in predicting both parent‐ and self‐report HRQL at 1 year after diagnosis. Larger prospective studies are needed to confirm these findings. Cognitive remediation or/and pharmacological intervention, particularly aimed at children with infratentorial tumours may improve attention and subsequently HRQL and both merit further investigation. Pediatr Blood Cancer. 2010;55:1377–1385. © 2010 Wiley‐Liss, Inc.
Treatment of childhood kaposiform hemangioendothelioma with sirolimus
Authors: Blatt Julie , Stavas Joseph , Moats‐Staats Billie , Woosley John , Morrell Dean S. 
Published: October 2010

AbstractSirolimus (Rapamune), a mammalian target of Rapamycin (mTOR) inhibitor, which has been used extensively in children following solid organ transplantation, has been demonstrated to have anti‐angiogenic activity in pre‐clinical models. Limited experience suggests that it may have application to the treatment of vascular lesions. We describe our experience with a 1‐year‐old female with a kaposiform hemangioendothelioma and Kasabach–Merritt phenomenon who had rapid and dramatic response to sirolimus (0.1 mg/kg/day). This case provides further rationale for clinical trials of sirolimus in the treatment of vascular lesions. Pediatr Blood Cancer. 2010;55:1396–1398. © 2010 Wiley‐Liss, Inc.
Quantitative analysis of major dibenzocyclooctane lignans in schisandrae fructus by online TLC‐DART‐MS
Authors: Kim Hye Jin , Oh Myung Sook , Hong Jongki , Jang Young Pyo 
Published: October 2010

AbstractIntroduction – Direct analysis in real time (DART) ion source is a powerful ionising technique for the quick and easy detection of various organic molecules without any sample preparation steps, but the lack of quantitation capacity limits its extensive use in the field of phytochemical analysis.Objective – To improvise a new system which utilize DART‐MS as a hyphenated detector for quantitation.Methodology – A total extract of Schisandra chinensis fruit was analyzed on a TLC plate and three major lignan compounds were quantitated by three different methods of UV densitometry, TLC‐DART‐MS and HPLC‐UV to compare the efficiency of each method. To introduce the TLC plate into the DART ion source at a constant velocity, a syringe pump was employed. The DART‐MS total ion current chromatogram was recorded for the entire TLC plate. The concentration of each lignan compound was calculated from the calibration curve established with standard compound.Results – Gomisin A, gomisin N and schisandrin were well separated on a silica‐coated TLC plate and the specific ion current chromatograms were successfully acquired from the TLC‐DART‐MS system. The TLC‐DART‐MS system for the quantitation of natural products showed better linearity and specificity than TLC densitometry, and consumed less time and solvent than conventional HPLC method.Conclusion – A hyphenated system for the quantitation of phytochemicals from crude herbal drugs was successfully established. This system was shown to have a powerful analytical capacity for the prompt and efficient quantitation of natural products from crude drugs. Copyright © 2010 John Wiley & Sons, Ltd.
Binding analysis of peptides that recognize preferentially cis‐azobenzene groups of synthetic polymers
Authors: Chen J. , Serizawa T. , Komiyama M. 
Published: October 2010

AbstractPeptides identified by the PD method against a film surface composed of azobenzene‐containing synthetic polymers were characterized by QCM measurements. Among the four peptides analyzed, the c16 peptide with the sequence Trp‐His‐Thr‐Leu‐Pro‐Asn‐Ala showed the highest binding affinity to the films rich in cis‐azobenzene groups. SPR served to determine the association/dissociation constants of the c16 peptide against the trans‐ and cis‐azobenzene groups. The binding constants were estimated to be 1.3 × 105 and 1.4 × 106/M, respectively, indicating a high specificity of the c16 peptide for cis‐azobenzene conformer. Then mutants of the c16 peptide were synthesized and characterized to gain information on the structural requirements for the cis‐form specificity. An Ala‐scan clearly revealed that all the amino acid residues of the c16 peptide were essential for the specificity. More importantly, the results with peptides of inverted sequence and containing Gly insertions confirmed that the specificity is strictly derived from the primary sequence of the c16 peptide. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd.
Facile synthesis of peptide nucleic acids and peptide nucleic acid‐peptide conjugates on an automated peptide synthesizer
Authors: Joshi Rajendra , Jha Deepti , Su Wu , Engelmann Joern 
Published: October 2010

AbstractPeptide nucleic acids (PNAs) are DNA mimics with a neutral peptide backbone instead of the negatively charged sugar phosphates. PNAs exhibit several attractive features such as high chemical and thermal stability, resistance to enzymatic degradation, and stable binding to their RNA or DNA targets in a sequence‐specific manner. Therefore, they are widely used in molecular diagnosis of antisense‐targeted therapeutic drugs or probes and in pharmaceutical applications. However, the main hindrance to the effective use of PNAs is their poor uptake by cells as well as the difficult and laborious chemical synthesis. In order to achieve an efficient delivery of PNAs into cells, there are already many published reports of peptides being used for transport across the cell membrane. In this protocol, we describe the automated as well as cost‐effective semi‐automated synthesis of PNAs and PNA‐peptide constructs on an automated peptide synthesizer. The facile synthesis of PNAs will be helpful in generating PNA libraries usable, e.g. for high‐throughput screening in biomolecular studies. Efficient synthetic schemes, the automated procedure, the reduced consumption of costly reagents, and the high purity of the products are attractive features of the reported procedure. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd.
Transit migration: A blurred and politicised concept
Authors: Düvell Franck 
Published: October 2010

ABSTRACTThis article examines first the emergence and construction of the concept of transit migration and the political framework and discourses that brought about this concept. It goes on analysing how this reinforces the EU's efforts to externalise its migration policy and integrate non‐EU countries into a comprehensive migration control policy. Second, it critically surveys the state of the art, analyses causes and conditions of transit migration, identifies its geography and discusses some methodological and analytical pitfalls and difficulties of researching transit migration. Finally, in the conclusions a structuralist approach is taken and it is suggested that a clear‐cut typology could be developed by rigid comparison. Copyright © 2010 John Wiley & Sons, Ltd.
Cytotoxic effects induced by combination of heliantriol B2 and dequalinium against human leukemic cell lines
Authors: Vela Gurovic M. Soledad , Díaz Lanza A. María , Boyano Adánez María del Carmen , Estañ Omaña M. Cristina , Gañán Gómez Irene , Murray A. Paula , Sancho López Pilar 
Published: October 2010

AbstractNatural occurring compounds are considered an important source of antitumoral agents. In the present study, the cytotoxic potential of three pentacyclic triterpenes isolated from Chuquiraga erinacea (Asteraceae), against the human leukemic cell lines NB4 and K562 was assessed. Heliantriol B2 (HB2) showed the highest cytotoxic activity after 24 h treatment showing IC50 values of 1.98 ± 0.12 µm and 3.52 ± 0.14 µm for NB4 and K562 cells, respectively. This activity was higher than that of the reference compound dequalinium (DQA). Apoptosis and necrosis induced by HB2 in both NB4 and K562 cell lines were analysed by Annexin V/PI labeling. Mitochondrial alterations including reactive oxygen species (ROS) production and mitochondrial transmembrane potential (ΔΨm) were also tested. The results demonstrated that HB2 induced cell death by apoptosis and necrosis and showed enhanced cytotoxic effects in combination with DQA. Besides, HB2 induced ROS overproduction in NB4 cells and a slight decrease of ΔΨm. Consequently, our findings prompt further studies on the HB2 mechanism of action and its selectivity to tumor cells in order to assess the potential of HB2 as an agent for cancer treatment. Copyright © 2010 John Wiley & Sons, Ltd.
A theoretical study on decomposition and rearrangement reaction mechanism of trichloroacetyl chloride (CCl3COCl)
Authors: Shankar R. , Kolandaivel P. , Senthilkumar K. 
Published: October 2010

AbstractIn the present study, the possible decomposition and rearrangement reaction profile of trichloroacetyl chloride have been studied using UMP2/6‐311++G (2d, 2p) level of ab initio and UB3LYP/6‐311++G (2d, 2p) level of density functional theory methods. The harmonic vibrational frequencies were calculated at the same level of theory used for the characterization of stationary points and zero‐point vibrational energy corrections. The potential energy barrier and activation energy between each step of the reaction have been calculated for the seven possible reaction pathways (Ia–c, IIa–b, IIIa–b). The trichloroacetyl chloride is an asymmetric ketone where the two α bonds of acetyl chloride, the CC and CCl bonds are strong with dissociation energy of 72 kcal/mol. The phosgene (COCl2), dichloroketene (CCl2CO), carbon dichloride (CCl2), carbon tetrachloride (CCl4), and carbon monoxide (CO) are the major dominant products on the decomposition of the trichloroacetyl chloride. These resultant products are more hazards than the parent trichloroacetyl chloride molecules. The positive value of the reaction energy indicate that the overall reaction profile is found to be endothermic at the UMP2 and UBLYP/6‐311++G(2d, 2p) levels of theory, respectively, at UMP2/6‐311G** optimized geometry. © 2010 Wiley Periodicals, Inc. Int J Quantum Chem, 2011
Quantum confinement effects on the surface enhanced Raman spectra of hybrid systems molecule‐TiO2 nanoparticles
Authors: Tarakeshwar Pilarisetty , Finkelstein‐Shapiro Daniel , Rajh Tijana , Mujica Vladimiro 
Published: October 2010

AbstractThe role of quantum confinement, size, and solvent effects on the surface enhanced Raman spectra of biologically important molecules absorbed on semiconducting titanium dioxide (TiO2) nanoparticles is investigated using density functional calculations. The results obtained for both the gas phase and solvated systems indicate significant changes in the electronic structure and the Raman spectra of molecules like formic acid and dopamine, when they are adsorbed on small TiO2 nanoparticles. A number of distinctive features that are determined by the formation of a charge‐transfer complex at the nanoparticle‐molecule interface can be noted in the Raman spectra. Both the spectra and the electronic properties are strongly size dependent and are also sensitive to the presence of the solvent and the nature of adsorbate interaction. Although these calculations reinforce recent experimental findings on the role of quantum confinement, they also pose new questions about the extension of collective effects and the effect of pH and other environmental variables. © 2010 Wiley Periodicals, Inc. Int J Quantum Chem, 2010
O‐loss photodissociation of the OCS+ ion in the low‐lying electronic states studied using multiconfiguration second‐order perturbation theory
Authors: Dong Hua , Chen Bo‐Zhen , Huang Ming‐Bao , Chang Hai‐Bo 
Published: October 2010

AbstractThe CASPT2 potential energy curves (PECs) for O‐loss dissociation from the X2Π, A2Π, B2Σ+, C2Σ+, 14Σ−, 12Σ−, and 14Π states of the OCS+ ion were calculated. The PEC calculations indicate that X2Π, 14Σ−, 12Σ−, and 14Π correlate with CS+(X2Σ+) + O(3Pg); A2Π and B2Σ+ correlate with CS+(A2Π) + O(3Pg); and C2Σ+ probably correlates with CS+(X2Σ+) + O(1Dg). The CASSCF minimum energy crossing point (MECP) calculations were performed for the C2Σ+/14Σ−, C2Σ+/14Π, A2Π/14Σ−, A2Π/12Σ−, A2Π/14Π, and B2Σ+/12Σ− state pairs and the spin‐obit couplings were calculated at the located MECPs. A conical intersection point between the B2Σ+ and C2Σ+ potential energy surfaces was found at the CASSCF level. Based on our calculations, seven O‐loss predissociation processes of the C2Σ+ state are suggested and an appearance potential value of 7.13 eV for the CS+ + O product group is predicted. © 2010 Wiley Periodicals, Inc. Int J Quantum Chem, 2011
Bounds to electronic expectation values for atomic and molecular systems
Authors: Marmorino M. G. , Cassella Kayleigh 
Published: October 2010

AbstractA generalization of a method to calculate lower bounds to expectation values of non‐negative observables is presented. We consider bounds to three electronic expectation values 〈r2〉, 〈r〉, and 〈r−1〉 in the helium atom as an example. For both 〈r2〉 and 〈r〉, we are able to calculate improved lower bounds. The lower bound to 〈r−1〉 does not improve, but we are able to calculate an upper bound which is much closer to the expectation value than the lower bound. Although our generalization allows for improved bounds and/or upper bounds, these bounds to general observables are much less precise than energy bounds and even the expectation values calculated from variational wave functions. © 2010 Wiley Periodicals, Inc. Int J Quantum Chem, 2011
A robot to detect and control broad‐leaved dock (Rumex obtusifolius L.) in grassland
Authors: van Evert Frits K. , Samsom Joost , Polder Gerrit , Vijn Marcel , Dooren Hendrik‐Jan van , Lamaker Arjan , van der Heijden Gerie W.A.M. , Kempenaar Corné , van der Zalm Ton , Lotz Lambertus A.P. 
Published: October 2010

AbstractBroad‐leaved dock is a common and troublesome grassland weed with a wide geographic distribution. In conventional farming the weed is normally controlled by using a selective herbicide, but in organic farming manual removal is the best option to control this weed. The objective of our work was to develop a robot that can navigate a pasture, detect broad‐leaved dock, and remove any weeds found. A prototype robot was constructed that navigates by following a predefined path using centimeter‐precision global positioning system (GPS). Broad‐leaved dock is detected using a camera and image processing. Once detected, weeds are destroyed by a cutting device. Tests of aspects of the system showed that path following accuracy is adequate but could be improved through tuning of the controller or adoption of a dynamic vehicle model, that the success rate of weed detection is highest when the grass is short and when the broad‐leaved dock plants are in rosette form, and that 75% of weeds removed did not grow back. An on‐farm field test of the complete system resulted in detection of 124 weeds of 134 encountered (93%), while a weed removal action was performed eight times without a weed being present. Effective weed control is considered to be achieved when the center of the weeder is positioned within 0.1 m of the taproot of the weed—this occurred in 73% of the cases. We conclude that the robot is an effective instrument to detect and control broad‐leaved dock under the conditions encountered on a commercial farm. © 2010 Wiley Periodicals, Inc.
Memory functioning in post‐traumatic stress disorder: objective findings versus subjective complaints
Authors: Carlozzi Noelle E. , Reese‐Melancon Celinda , Thomas David G. 
Published: October 2010

AbstractAlthough memory complaints are common in post‐traumatic stress disorder (PTSD), the only published study of objective and subjective memory in PTSD by Roca and Freeman indicates that subjective complaints may not accurately reflect objective performance. The present study examined memory in 21 PTSD patients, 20 combat controls and 23 non‐combat controls using two objective memory measures (Rey Auditory Verbal Learning Test; Backward Digit Span) and one subjective measure (Memory Functioning Questionnaire). Analysis of variances (ANOVAs) and analysis of co‐variances generally did not reveal group differences for objective memory performance. For subjective memory complaints, ANOVAs indicated group differences; PTSD participants reported more memory problems than controls. These differences disappeared, however, when depression was included as a covariate. Also, we provide some preliminary evidence that depression may mediate the relationship between PTSD symptomatology and subjective memory complaints. Findings suggest that reductions in depressive symptomatology in PTSD may be associated with decreased subjective memory complaints. Copyright © 2010 John Wiley & Sons, Ltd.
Costs and benefits of supportive versus disciplinary emotion regulation strategies in teachers
Authors: Barber Larissa K. , Grawitch Matthew J. , Carson Russell L. , Tsouloupas Costas N. 
Published: October 2010

AbstractUsing 659 K‐12 teachers, this study explored the extent to which well‐being outcomes were affected by differential emotion regulation strategies (surface versus deep acting) for positive emotion expression (supportive display rules) versus negative emotion expression (disciplinary display rules). Analyses showed that almost half of the teachers reported disciplinary display rules as important for effectively doing their job (as compared with 97% for supportive display rules), and these perceptions were associated with increased disciplinary surface and deep acting strategies. A confirmatory factor analysis supported the proposed four‐factor structure of emotional regulation strategies, and provided the best fit in relation to two alternative models (surface versus deep acting and supportive versus disciplinary). A structural equation model revealed that both supportive surface acting and disciplinary surface acting positively predicted emotional exhaustion, and both negatively predicted personal accomplishment. However, only supportive deep acting had a positive relationship with personal accomplishment. Disciplinary deep acting was unrelated to the study outcomes. These findings indicate that disciplinary emotion regulation may have the same costs to emotional exhaustion in teachers as supportive regulation, but fewer benefits in terms of increasing personal accomplishment. Copyright © 2010 John Wiley & Sons, Ltd.
Gaucher disease in sheep
Authors: Karageorgos Litsa , Lancaster Malcolm J. , Nimmo Judith S. , Hopwood John J. 
Published: October 2010

AbstractGaucher disease, an autosomal recessive lysosomal storage disorder caused by mutations in the β‐glucocerebrosidase gene, was recently discovered in sheep on a “Southdown” sheep stud in Victoria, Australia. Clinical signs include neuropathy, thickened leathery skin, and ichthyosis, with lambs unable to stand from birth. Affected lambs were found to be deficient in glucocerebrosidase activity, and mutational analysis found them to be homozygous for the missense mutations c.1142G>A (p.C381Y) and c.1400C>T (p.P467L). In addition, four silent mutations were detected (c.777C>A [p.Y259Y], c1203A>G [p.Q401Q], c.1335T>C [p.I445I], c.1464C>G [p.L488L]). The human equivalent [C342Y] to the C381Y mutation leads to an acute neuronopathic phenotype in patients. Identification of an acute neuronopathic form of Gaucher disease in sheep provides a large animal model that will enable studies of pathology and evaluation of therapies to treat this common lysosomal storage disorder.
Excess risk of adverse pregnancy outcomes in women with porphyria: a population‐based cohort study
Authors: Tollånes Mette Christophersen , Aarsand Aasne Karine , Sandberg Sverre 
Published: October 2010

AbstractThe porphyrias comprise a heterogeneous group of rare, primarily hereditary, metabolic diseases caused by a partial deficiency in one of the eight enzymes involved in the heme biosynthesis. Our aim was to assess whether acute or cutaneous porphyria has been associated with excess risks of adverse pregnancy outcomes. A population‐based cohort study was designed by record linkage between the Norwegian Porphyria Register, covering 70% of all known porphyria patients in Norway, and the Medical Birth Registry of Norway, based on all births in Norway during 1967–2006. The risks of the adverse pregnancy outcomes preeclampsia, delivery by caesarean section, low birth weight, premature delivery, small for gestational age (SGA), perinatal death, and congenital malformations were compared between porphyric mothers and the rest of the population. The 200 mothers with porphyria had 398 singletons during the study period, whereas the 1,100,391 mothers without porphyria had 2,275,317 singletons. First‐time mothers with active acute porphyria had an excess risk of perinatal death [adjusted odds ratio (OR) 4.9, 95% confidence interval (CI) 1.5–16.0], as did mothers with the hereditable form of porphyria cutanea tarda (PCT) (3.0, 1.2–7.7). Sporadic PCT was associated with an excess risk of SGA [adjusted relative risk (RR) 2.0, 1.2–3.4], and for first‐time mothers, low birth weight (adjusted OR 3.4, 1.2–10.0) and premature delivery (3.5, 1.2–10.5) in addition. The findings suggest women with porphyria should be monitored closely during pregnancy.