bluuebunny/crossref_metadata_2025_split huggingface dataset:
New protein isoforms identified within Arabidopsis thaliana seed oil bodies combining chymotrypsin/trypsin digestion and peptide fragmentation analysis
Authors: Vermachova Martina , Purkrtova Zita , Santrucek Jiri , Jolivet Pascale , Chardot Thierry , Kodicek Milan 
Published: June 2011

AbstractPlant seed oil bodies, subcellular lipoprotein inclusions providing storage reserves, are composed of a neutral lipid core surrounded by a phospholipid monolayer with several integrated proteins that play a significant role in stabilization of the particles and probably also in lipid mobilization. Oil bodies' proteins are generally very hydrophobic, due to the long uncharged sequences anchoring them into the lipid core, which makes them extremely difficult to handle and to digest successfully. Although oil bodies have been intensively studied during last decades, not all their proteins have been identified yet. To overcome the problems connected with their identification, a method based on SDS‐PAGE, in‐gel digestion and LC‐MS/MS analysis was used. Digestion was carried out with trypsin and chymotrypsin, single or in combination, which increased significantly the number of identified peptides, namely the hydrophobic ones. Thanks to this methodology it was possible to achieve an extensive coverage of proteins studied, to analyze their N‐terminal modifications and moreover, to detect four new oil bodies' protein isoforms, which demonstrates the complexity of oil bodies' protein composition.
Proteome characterization of sea star coelomocytes – The innate immune effector cells of echinoderms
Authors: Franco Catarina F. , Santos Romana , Coelho Ana V. 
Published: June 2011

AbstractSea star coelomic fluid is in contact with all internal organs, carrying signaling molecules and a large population of circulating cells, the coelomocytes. These cells, also known as echinoderm blood cells, are responsible for the innate immune responses and are also known to have an important role in the first stage of regeneration, i.e. wound closure, necessary to prevent disruption of the body fluid balance and to limit the invasion of pathogens. This study focuses on the proteome characterization of these multifunctional cells. The identification of 358 proteins was achieved using a combination of two techniques for protein separation (1‐D SDS‐PAGE followed by nanoLC and 2‐D SDS‐PAGE) and MALDI‐TOF/TOF MS for protein identification. To our knowledge, the present report represents the first comprehensive list of sea star coelomocyte proteins, constituting an important database to validate many echinoderm‐predicted proteins. Evidence for new pathways in these particular echinoderm cells are also described, and thus representing a valuable resource to stimulate future studies aiming to unravel the homology with vertebrate immune cells and particularly the origins of the immune system itself.
Lymphatic Tumor Emboli Detected by D2‐40 Immunostaining Can More Accurately Predict Lymph‐node Metastasis
Authors: Moriya Hiromitsu , Ohbu Makoto , Kobayashi Nobuyuki , Tanabe Satoshi , Katada Natsuya , Futawatari Nobue , Sakuramoto Shinichi , Kikuchi Shiro , Okayasu Isao , Watanabe Masahiko 
Published: June 2011

AbstractBackgroundResected specimens of superficial squamous cell carcinoma of the esophagus (SSCCE) underwent D2‐40 immunostaining to accurately assess lymphatic tumor emboli (LY) and to analyze correlations between LY and lymph node metastasis (N). This present study was designed to determine the accuracy of LY grade for predicting the risk of N.Materials and methodsWe studied 75 patients with SSCCE who underwent surgical resection of their tumors. Resected specimens were sliced into continuous sections at 5 mm intervals. Intramucosal cancers are classified into three groups (m1, m2, m3), and submucosal cancers are also divided into three groups (sm1, sm2, sm3). The numbers of LY present in lymphatic ducts on D2‐40 immunostaining, venous tumor emboli (V) on CD34 immunostaining, and lymphatic tumor emboli (ly) and V on hematoxylin‐eosin staining (HE) and elastica van Gieson staining (EVG) were counted for each case. The presence of lymphatic tumor emboli was graded according to the total number of LY per case as follows: 0, LY0; 1 to 2, LY1; 3 to 9, LY2; and 10 or more, LY3.ResultsAll m1 and m2 cases were LY− and N− Lymphatic tumor emboli were present in 54% of m3 cases, 70% of sm1 cases, 54% of sm2 cases, and 75% of sm3 cases. Determination of N was positive in 18% of m3 cases, 47% of sm1 cases, 36% of sm2 cases, and 62% of sm3 cases. The frequency of LY significantly correlated with the number of N (p < 0.0001). Multiple regression analysis showed that only LY and V significantly correlated with N. When the detection rate of N was compared between LY and ly, LY was superior to ly in terms of specificity, accuracy, positive predictive value, and false positive rate. As for LY grade, N was positive in 39.1% of LY1 cases, 81.8% of LY2 cases, and 100% of LY3 cases. Even in LY‐, N was positive in one sm1 case and in two sm2 cases. In the sm1 case, the depth of invasion was 350 μm from the lower margin of the muscularis mucosae.ConclusionsEvaluation of lymphatic invasion on the basis of LY is more accurate for the prediction of N than conventional techniques, and LY grade strongly correlates with N. In patients with SSCCE, mucosal cancers (m1, m2, and m3) and submucosal cancers with a depth of invasion of ≤200 μm from the lower margin of the muscularis mucosae on endoscopic mucosal resection have a low risk of N if the number of LY is 0. Endoscopic mucosal resection alone can provide good treatment outcomes in such patients.
Learning Curve of Transumbilical Single Incision Laparoscopic Cholecystectomy (SILS): A Preliminary Study of 80 Selected Patients with Benign Gallbladder Diseases
Authors: Qiu Zhengjun , Sun Jing , Pu Ying , Jiang Tao , Cao Jun , Wu Weidong 
Published: June 2011

AbstractBackgroundTransumbilical single incision laparoscopic surgery (SILS) is a new laparoscopic procedure in which only one transumbilical incision is made, demonstrated as a scarless procedure. Here we report a single‐center preliminary experience of transumbilical single incision laparoscopic cholecystectomy (SILC) in the treatment of benign gallbladder diseases, defining a single surgeon’s learning curve.MethodsA total of 80 patients underwent SILC successfully by a single experienced laparoscopic surgeon. The operation was performed following the routine LC procedure. Then the perioperative demographics were recorded and the operative time was used to define the learning curve.ResultsThe study group included 27 male and 53 female patients with gallstones (56 cases), cholesterol polyps (16 cases), an adenomatous polyp (3 cases), adenomyomatosis (1 case), or complex diseases (4 cases), and all consented to undergo SILC. No patient was converted to normal LC or open surgery. There were no perioperative port‐related or surgical complications. The average operative time was 46.9 ± 14.6 min. The average postoperative hospital stay was 1.8 ± 1.3 days. The learning curve of the SILC procedures for this series of selected patients confirmed that SILC is a feasible, safe, and effective approach to the treatment of benign gallbladder diseases.ConclusionsFor experienced laparoscopic surgeons, SILC is an easy and safe procedure. Patients benefit from milder pain, a lower incidence of port‐related complications, better cosmesis, and fast recovery. The SILC procedure may become another option for the treatment of benign gallbladder diseases for selected patients.
Compliance with and effects of preoperative immunonutrition in patients undergoing pancreaticoduodenectomy
Authors: Shirakawa Hirofumi , Kinoshita Taira , Gotohda Naoto , Takahashi Shinichiro , Nakagohri Toshio , Konishi Masaru 
Published: June 2011

AbstractBackground/purposeThis study was conducted to ascertain the feasibility and effectiveness of preoperative enteral immunonutrition using an immune‐enhanced formula (Impact) in patients undergoing pancreaticoduodenectomy.MethodsTwenty‐five patients undergoing an elective pancreaticoduodenectomy were asked to ingest Impact for 5 days (750 mL/day) prior to surgery in addition to their normal diets. We retrospectively compared the early postoperative outcomes of the Impact group (n = 18), which consisted of patients who fully complied with the study protocol, and a control group (n = 13), which consisted of patients who had not ingested Impact prior to surgery.ResultsOverall, 82.6% of the patients complied with the preoperative oral ingestion of Impact; all but four patients tolerated a daily intake of 750 mL. While the clinical backgrounds of the Impact and control groups were not significantly different, the frequency of incisional wound infection was lower (0 vs. 30.8%,p = 0.012) and the change in systemic severity as evaluated using the acute physiology and chronic health evaluation (APACHE)‐II scoring system was milder (p = 0.033) in the Impact group than in the control group.ConclusionThe preoperative oral ingestion of Impact was well tolerated and appeared to be effective for preventing incisional wound infection and reducing the response to surgical stress in patients undergoing a pancreaticoduodenectomy.
Examining the diversity maxima of marine macrophytes and their relationship with a continuous environmental stress gradient in the Northern Ryukyu Archipelago
Authors: Nishihara Gregory N. , Terada Ryuta 
Published: June 2011

AbstractIn coastal marine ecosystems, the environmental stress model (ESM) predicts that the central measures (i.e., the mean or median) of species richness are highest at intermediate stresses (e.g., intermediate levels of light and wave exposure). It is now appropriate to examine ESM over larger spatial scales beyond a single shoreline, using a continuous stress scale, and non‐central measures of species richness. The relationship between marine macrophyte richness and a continuous stress gradient (i.e., hydrodynamic stress) from 210 sites in the Northern Ryukyu Archipelago were examined. Expectile regression splines were used to determine how non‐central measures of richness vary with stress. Species richness peaked at intermediate stresses and this feature was strongest at the higher expectiles (i.e., in the upper tails of the distribution of species richness). The fitted expectile regressions converged at the highest and lowest stress, and were widely spaced at intermediate values. This suggests that environmental stress, as determined, is the process that controls species richness at low and high stress. A provisional analysis assuming a Gumbel distribution to model the extreme values of species richness mirrored the patterns elucidated by the expectile regression. Expectile regression and extreme value approaches may provide a means of predicting the occurrence of species richness maxima at the regional scale.
Functional and Emotional Results Differ after Aseptic vs Septic Revision Hip Arthroplasty
Authors: Boettner Friedrich , Cross Michael B. , Nam Denis , Kluthe Tobias , Schulte Miriam , Goetze Christian 
Published: June 2011

Background It is widely believed that a deep implant infection leads to poor functional and emotional outcomes following total hip arthroplasty. Questions/Purpose The purpose of this retrospective comparative review was to determine if patients who undergo two-stage, septic revision hip arthroplasty will have decreased emotional and general health scores, in addition to decreased function, compared to the aseptic revision group. Patients and Methods One hundred forty-five of 195 patients who underwent aseptic total hip revision for aseptic loosening (mean follow-up = 61 months) and 45 of 73 patients who underwent two-stage, septic revision hip arthroplasty (mean follow-up = 48 months) met the inclusion criteria and had a technically successful outcome. All patients were retrospectively evaluated using Harris Hip Scores (HHS), ad hoc questions, and the SF-36 Health Survey. Results The average HHS were 73.2 ± 20.5 (aseptic) and 57.4 ± 20.6 (septic). Significant differences in the SF-36 Health Survey were found between the two groups in: physical functioning ( p = 0.026) and role limitations due to physical health ( p = 0.004). No significant difference in SF-36 scores was seen in: Energy/Fatigue, General Health Perception, Personal or Emotional Problems, Role Limitations due to Emotional Well Being, Social Functioning, and Bodily Pain. Conclusions Two-stage, septic revision produces a poor functional outcome compared to aseptic revision; however, the overall impact of a septic revision emotionally and socially was not significantly different than patients undergoing aseptic revision.
Cold‐Pressed Pumpkin Seed Oil Antioxidant Activity as Determined by a DC Polarographic Assay Based on Hydrogen Peroxide Scavenge
Authors: Gorjanović Stanislava Ž. , Rabrenović Biljana B. , Novaković Miroslav M. , Dimić Etelka B. , Basić Zorica N. , Sužnjević Desanka Ž. 
Published: June 2011

AbstractAntioxidant (AO) activity of cold pressed pumpkin (Cucurbita pepo L.) seed oil, produced from three naked and one hulled variety, was assessed using a DC polarographic assay based on a hydrogen peroxide scavenge (HPS). Results are expressed as the decrease of the anodic oxidation current of hydrogen peroxide obtained upon addition of methanolic extract of the investigated oils. Strict correlations of HPS and (1) radical scavenging capacity against the stable free radical 1,1‐diphenyl‐2‐picrylhydrazyl (DPPH) (0.99), (2) the induction period estimated by a Rancimat test (0.99) and (3) total phenolic content estimated by Folin‐Ciocalteu (FC) assay (0.99) were obtained. In addition, a significant correlation of HPS and the content of δ‐tocopherol (0.87), squalene (0.67) and color CIE a* (−0.89) was found. Based on the results reported, the polarographic assay was found to be suitable for determination of AO activity as an indicator of the quality and oxidative stability of oil.
Oxidative Stability of Polyunsaturated Edible Oils Mixed With Microcrystalline Cellulose
Authors: Saga Linda C. , Rukke Elling‐Olav , Liland Kristian Hovde , Kirkhus Bente , Egelandsdal Bjørg , Karlsen Jan , Volden Jon 
Published: June 2011

AbstractThe oxidative stability of mixtures of edible oils containing polyunsaturated fatty acids (PUFA) and microcrystalline cellulose (MCC) was investigated. The mixtures studied consisted of oils of either camelina (CAM), cod liver (CLO), or salmon (SO) mixed with either colloidal or powdered MCC. A 50:50 (w/w) ratio of oil:MCC resulted in an applicable mixture containing high levels of PUFA edible oil and dietary fiber. The oxidative stability of the formulated mixtures and the pure oils was investigated over a period of 28 days. The peroxide value (PV) was assessed as a parameter for primary oxidation products and dynamic headspace gas chromatography mass spectrometry (GC/MS) was used to analyze secondary volatile organic compounds (VOC). CAM and the respective mixtures were oxidatively stable at both 4 and 22 °C during the storage period. The marine oils and the respective mixtures were stable at 4 °C. At 22 °C, an increase in hydroperoxides was found, but no increase in VOC was detected during the time‐frame investigated. At 42 °C, prominent increases in PV and VOC were found for all oils and mixtures. Hexanal, a common marker for the degradation of n‐6 fatty acids, propanal and 2,4‐heptadienal (E,E), common indicators for the degradation of n‐3 fatty acids, were among the volatiles detected in the headspace of oils and mixtures. This study showed that a mixture containing a 50:50 ratio of oil:MCC can be obtained by a low‐tech procedure that does not induce oxidation when stored at low temperatures during a period of 1 month.
Life Shocks and Crime: A Test of the “Turning Point” Hypothesis
Authors: Corman Hope , Noonan Kelly , Reichman Nancy E. , Schwartz-Soicher Ofira 
Published: June 2011

Abstract
Other researchers have posited that important events in men’s lives—such as employment, marriage, and parenthood—strengthen their social ties and lead them to refrain from crime. A challenge in empirically testing this hypothesis has been the issue of self-selection into life transitions. This study contributes to this literature by estimating the effects of an exogenous life shock on crime. We use data from the Fragile Families and Child Wellbeing Study, augmented with information from hospital medical records, to estimate the effects of the birth of a child with a severe health problem on the likelihood that the infant’s father engages in illegal activities. We conduct a number of auxiliary analyses to examine exogeneity assumptions. We find that having an infant born with a severe health condition increases the likelihood that the father is convicted of a crime in the three-year period following the birth of the child, and at least part of the effect appears to operate through work and changes in parental relationships. These results provide evidence that life events can cause crime and, as such, support the “turning point” hypothesis.
Shock wave interaction with convex circular cylindrical surfaces – ERRATUM
Authors: SKEWS BERIC W. , KLEINE HARALD 
Published: June 2011

In Skews & Kleine (2010) the correct figure 2 with the corresponding caption should appear as follows.
Figure 2.Wall angles at which Mach reflection becomes evident for different radii surfaces and shock strengths. The solid curve is the sonic criterion for plane walls. Previous data are from Ben-Dor (2007).
ON HARMONIC BLOCH SPACES IN THE UNIT BALL OF ℂn
Authors: CHEN SH. , WANG X. 
Published: June 2011

AbstractIn this paper, our main aim is to discuss the properties of harmonic mappings in the unit ball 𝔹n. First, we characterize the harmonic Bloch spaces and the little harmonic Bloch spaces from 𝔹n to ℂ in terms of weighted Lipschitz functions. Then we prove the existence of a Landau–Bloch constant for a class of vector-valued harmonic Bloch mappings from 𝔹n to ℂn.
The Black Church: Its Development and Present Impact
Authors: McKinney Richard I. 
Published: June 2011

The Black church, historically, is the most significant institution in the Black community.I. History and Development of the Black ChurchA. There were differences of opinion with respect to whether slaves should be Christianized.B. The Christian religion was accepted by the slaves because it offered hope and salvation.C. Although at first religious instruction was given the slaves, this was later discontinued, then renewed after the Revolutionary War.D. Three major denominations split over the question of slavery.E. Independent churches were established by free Negroes in the North.
One Jesus and Four Primitive Gospels
Authors: Koester Helmut H. 
Published: June 2011

To deal with the problem of the “historical” Jesus is to deal with the synoptic Gospels (with occasional appropriations of Johan-nine material).To use the apocryphal Gospels does not seem to be advisable, since their inclusion is beset with a number of notorious difficulties. First of all, any attempt to recover historical material from the vast sea of noncanonical tradition has proved to be an arduous labor yielding only negligible results.
High frequency hearing loss in the elderly: effect of age and noise exposure in an Italian group
Authors: Ciorba A , Benatti A , Bianchini C , Aimoni C , Volpato S , Bovo R , Martini A 
Published: June 2011

AbstractObjective:To describe the effect of age and noise on high frequency hearing thresholds in an Italian population aged 70 years and older, in order to investigate the interaction between presbycusis and noise exposure.Methods:We compared 460 subjects: 367 affected by presbycusis alone (204 women and 163 men) and 93 affected by presbycusis and noise exposure (eight women and 85 men). Pure tone average hearing thresholds, for each ear, were compared between groups, and between sexes and ages within groups.Results:A slight threshold difference was found between the two groups at 4 kHz. After adjusting for age and gender, this difference was found to be related only to differing patient age. Men's and women's thresholds differed significantly in both groups, especially at high frequencies, at which threshold deterioration was worse in men than women.Conclusion:The threshold differences between patients with presbycusis with and without noise exposure were limited. Larger studies are needed to assess the relative effects of ageing and noise exposure on hearing thresholds.
Pyriform sinus haemangioma: an unusual presentation of an unusual condition
Authors: Hussain K , Makura Z G G 
Published: June 2011

AbstractObjective:We present a rare case of an unusual presentation of a pyriform sinus haemangioma in a normally healthy, Caucasian woman, including our management and a review of the literature.Case report:A 44-year-old woman presented complaining of bringing up fresh blood whilst brushing her teeth, dysphonia, food sticking in her throat, and epigastric pain for the preceding 12 months, accompanied by a 17.5 kg weight loss. She underwent pre-operative computed tomography and subsequent complete excision of a pyriform sinus haemangioma using CO2laser.Discussion:Haemangiomas are congenital vascular malformations and can affect any part of the body. They are not prevalent in adults and are rarely found in the pyriform sinus. Their common presenting symptoms and management have been previously documented; however, the presented case is unusual in both its presentation and management.
Dislocation of the inferior turbinates: a rare complication of nasal surgery, presenting as obstructive sleep apnoea
Authors: Gupta V , Singh H , Gupta M , Singh S 
Published: June 2011

AbstractObjective:We report a very rare complication of nasal surgery: dislocation of the inferior turbinates into the nasopharynx, presenting as sleep apnoea and persistent nasal obstruction.Case report:A 56-year-old woman presented with a history of obstructive sleep apnoea for one year and nasal obstruction for seven years. She had undergone nasal surgery one year previously. Non-contrast computed tomography of the nose and paranasal sinuses showed a mass near the posterior choana on both sides, confirmed by nasal endoscopy. The displaced turbinates were removed, after resecting their attachment at the posterior choanae, and the patient's symptoms were relieved.Conclusion:This is a very rare complication of nasal surgery; to the best of our knowledge, we report only the second published case in the English language literature. We suggest that such cases be closely followed up post-operatively.
Peter Fraser MA, MC, FBA (1918–2007)
Authors: Hornblower Simon 
Published: June 2011

This is a brief memoir of Peter Fraser, who was Director of the British School at Athens between 1968 and 1971, and a Hellenistic historian and epigraphist of international distinction.Παρουσιάζεται μία σύντομη βιογραφία του διεθνούς φήμης ιστορικού και επιγραφικού της Ελληνιστικής περιόδου, Peter Fraser, ο οποίος διετέλεσε Διευθυνιής της Βρετανικής Σχολής των Αθηνών μεταξύ των ετών 1968 και 1971.