bluuebunny/crossref_metadata_2025_split huggingface dataset:
Asymmetric multi-image encoding and hiding scheme with structured fingerprint phase masks using gyrator transform and phase-shifting digital holography
Authors: Kumar Rao Sonu , Nishchal Naveen K 
Published: April 2024

Abstract
We propose a novel technique for multi-image encryption and hiding schemes under an optical asymmetric framework using structured fingerprint phase masks (SFPMs) in the gyrator transform (GT) domain and three-step phase-shifting digital holography (PSDH). A SFPM contains unique features of fingerprint and structured phases of the optical vortex beam, which provides enhanced security in the cryptosystem. To encrypt multiple images, GT-based phase truncation and phase reservation techniques have been used in the first level of security, whereas three-step PSDH has been used to obtain the final cipher text. The cipher text is embedded in the host image to perform the watermarking process. In this process, the host is further decomposed into three parts in which anyone from the last two parts can be used for watermark embedding, and the first part is stored as the key. The use of polar decomposition in the watermarking process provides an additional layer of security. Numerical simulations and experimental results are presented to support the proposed scheme.
Quantum-enhanced hybrid feature engineering in thoracic CT image analysis for state-of-the-art nodule classification: an advanced lung cancer assessment
Authors: Shivwanshi Resham Raj , Nirala Neelamshobha 
Published: April 2024

Abstract
The intricate nature of lung cancer treatment poses considerable challenges upon diagnosis. Early detection plays a pivotal role in mitigating its escalating global mortality rates. Consequently, there are pressing demands for robust and dependable early detection and diagnostic systems. However, the technological limitations and complexity of the disease make it challenging to implement an efficient lung cancer screening system. AI-based CT image analysis techniques are showing significant contributions to the development of computer-assisted detection (CAD) systems for lung cancer screening. Various existing research groups are working on implementing CT image analysis systems for assessing and classifying lung cancer. However, the complexity of different structures inside the CT image is high and comprehension of significant information inherited by them is more complex even after applying advanced feature extraction and feature selection techniques. Traditional and classical feature selection techniques may struggle to capture complex interdependencies between features. They may get stuck in local optima and sometimes require additional exploration strategies. Traditional techniques may also struggle with combinatorial optimization problems when applied to a prominent feature space. This paper proposed a methodology to overcome the existing challenges by applying feature extraction using Vision Transformer (FexViT) and Feature selection using the Quantum Computing based Quadratic unconstrained binary optimization (QC-FSelQUBO) technique. This algorithm shows better performance when compared with other existing techniques. The proposed methodology showed better performance as compared to other existing techniques when evaluated by applying necessary output measures, such as accuracy, Area under roc (receiver operating characteristics) curve, precision, sensitivity, and specificity, obtained as 94.28%, 99.10%, 96.17%, 90.16% and 97.46%. The further advancement of CAD systems is essential to meet the demand for more reliable detection and diagnosis of cancer, which can be addressed by leading the proposed quantum computation and growing AI-based technology ahead.
The Pennsylvania Council of Censors and the Debate on the Guardian of the Constitution in the Early United States
Authors: Brown Angus Harwood 
Published: April 2024

Abstract
In 1776, Pennsylvania established an institution called the Council of Censors, which would be elected every seven years and was tasked with ensuring that the legislative, executive, and judicial branches of government had remained faithful to the constitution. None of the other thirteen colonies would create a similar institution, although Vermont would in 1777. Nor has the Council of Censors enjoyed a positive reputation among historians or constitutional scholars: Gordon Wood, for example, has attacked the institution as ‘a monster [pulled] out of Roman history’. Contemporaries agreed, and the body was abolished in Pennsylvania in 1790 after years of vociferous opposition and was criticized extensively at the Federal Convention in 1787. But the Council of Censors was a remarkably innovative institution, the first designed to enforce a written constitution, created decades before the Supreme Court’s assumption of the power of constitutional judicial review in 1803. This article presents a new history of the origins of the Council of Censors and its reception both in Pennsylvania and across the United States. It challenges prevailing accounts of the origins and purpose of the Council of Censors and argues that it was a product of a new theory of constitutionalism as the codification of popular sovereignty which emerged in the United States in the 1770s in response to the colonists’ fears about legislative overreach. Prior to the nineteenth century, it was only in Pennsylvania that this resulted in the creation of institutions to secure the supremacy of constitutional law over ordinary legislative power. As the final section of this article demonstrates, the idea that the constitution could be enforced against the legislative branch by an independent constitutional guardian—including the Supreme Court—was rejected at the Federal Convention precisely because of its framers’ antipathy to Pennsylvania’s radically democratic constitution.
Túngara frog call-timing decisions arise as internal rhythms interact with fluctuating chorus noise
Authors: Larter Luke C , Ryan Michael J 
Published: April 2024

Abstract
For chorusing males, optimally timing their calls relative to nearby rivals’ calls and fluctuations in background chorus noise is crucial for reproductive success. A caller’s acoustic environment will vary by chorus density and the properties of his chorus-mates’ calls and will fluctuate unpredictably due to chorusing dynamics emerging among his chorus-mates. Thus, callers must continuously monitor moment-to-moment fluctuations in the acoustic scene they perceive at the chorus for advantageous times to call. In live experimental choruses, we investigated the factors influencing túngara frog call-timing responses to chorus-mates’ calls on an interaction-by-interaction basis, revealing that intrinsic and extrinsic factors influenced call-timing decisions. Callers were more likely to overlap calls from smaller chorus-mates and chorus-mates at intermediate distances, as well as calls containing lower frequencies and exhibiting lower final amplitude minima. Consequently, variation among males in call properties led to variation in levels of call-interference received when calling in the same social environment. Additionally, callers were more likely to overlap chorus-mates’ calls after experiencing extended periods of inhibition and were less likely to overlap synchronized chorus-mates’ calls relative to single calls. In chorusing species, female choice is influenced by inter-caller dynamics, selecting for male call-timing strategies which, in turn, constitute the selective environment further refining these same strategies. Thus, understanding the specific factors driving call-timing decisions is essential for understanding how sexual selection operates in chorusing taxa.
Factors defining developmental competence of bovine oocytes collected for in vitro embryo production
Authors: Pytel Aleksandra Teresa , Żyżyńska-Galeńska Krystyna , Gajewski Zdzisław , Papis Krzysztof 
Published: April 2024

Abstract
Despite the currently relatively low effectiveness of producing bovine embryos in vitro, there is a growing interest in applying this laboratory method in the field of reproduction. Many aspects of the procedure need to be improved. One of the main problems is the inferior developmental competence of in vitro matured oocytes that are collected using the ovum pick-up method. The mechanisms of oocyte capacitation and maturation, as well as the in vivo conditions in which they grow and mature, should be carefully analyzed. A deliberate application of the identified mechanisms and beneficial factors affecting the in vitro procedures seems to be essential for achieving higher developmental competence of the oocytes that are subjected to fertilization. The results may be improved by developing and employing a laboratory maturation protocol that corresponds with appropriate preparation of donors before the ovum pick-up, an optimized hormonal treatment program, the appropriate size of ovarian follicles at the time of aspiration, and a fine-tuned coasting period.
Voices of Women Exposed to Honour-Based Violence: On Vulnerability, Needs and Support from Social Services
Authors: Olsson Helén 
Published: April 2024

Abstract
Swedish social services play a pivotal role in preventing men’s violence against women, including honour-based violence. This type of family-based violence is based on norms that disregard human rights. Individuals growing up in an honour context have limited possibilities to choose their own paths in life. The study comprises young women aged eighteen to twenty-five who look back on their vulnerable positions and the circumstances resulting in their seeking help from social services. They also share their experiences of the support that they had received from social services. Semi-structured interviews with ten women were conducted. The data were analysed through a qualitative content analysis. The findings show that the interviewees are not a homogenous group; circumstances, needs and exposure varied considerably between participants. Professional attention is necessary when threats ensue after divorce, for example, in the case of a bride price refund. Child perspectives must be better considered in the event of a divorce. Women or families that reject standards of honour are subject to harassment in public by people aiming to coerce them into adopting honour norms. Leaving the family was facilitated if one’s fears were taken seriously and experiences of being met with understanding and being well cared for.
Seizures exacerbate excitatory: inhibitory imbalance in Alzheimer’s disease and 5XFAD mice
Authors: Barbour Aaron J , Gourmaud Sarah , Lancaster Eunjoo , Li Xiaofan , Stewart David A , Hoag Keegan F , Irwin David J , Talos Delia M , Jensen Frances E 
Published: April 2024

Abstract
Approximately 22% of Alzheimer’s disease (AD) patients suffer from seizures, and the co-occurrence of seizures and epileptiform activity exacerbates AD pathology and related cognitive deficits, suggesting that seizures may be a targetable component of AD progression. Given that alterations in neuronal excitatory:inhibitory (E:I) balance occur in epilepsy, we hypothesized that decreased markers of inhibition relative to those of excitation would be present in AD patients. We similarly hypothesized that in 5XFAD mice, the E:I imbalance would progress from an early stage (prodromal) to later symptomatic stages and be further exacerbated by pentylenetetrazol (PTZ) kindling.
Post-mortem AD temporal cortical tissues from patients with or without seizure history were examined for changes in several markers of E:I balance, including levels of the inhibitory GABAA receptor, the sodium potassium chloride cotransporter 1 (NKCC1) and potassium chloride cotransporter 2 (KCC2) and the excitatory NMDA and AMPA type glutamate receptors. We performed patch-clamp electrophysiological recordings from CA1 neurons in hippocampal slices and examined the same markers of E:I balance in prodromal 5XFAD mice. We next examined 5XFAD mice at chronic stages, after PTZ or control protocols, and in response to chronic mTORC1 inhibitor rapamycin, administered following kindled seizures, for markers of E:I balance.
We found that AD patients with comorbid seizures had worsened cognitive and functional scores and decreased GABAA receptor subunit expression, as well as increased NKCC1/KCC2 ratios, indicative of depolarizing GABA responses. Patch clamp recordings of prodromal 5XFAD CA1 neurons showed increased intrinsic excitability, along with decreased GABAergic inhibitory transmission and altered glutamatergic neurotransmission, indicating that E:I imbalance may occur in early disease stages. Furthermore, seizure induction in prodromal 5XFAD mice led to later dysregulation of NKCC1/KCC2 and a reduction in GluA2 AMPA glutamate receptor subunit expression, indicative of depolarizing GABA receptors and calcium permeable AMPA receptors. Finally, we found that chronic treatment with the mTORC1 inhibitor, rapamycin, at doses we have previously shown to attenuate seizure-induced amyloid-β pathology and cognitive deficits, could also reverse elevations of the NKCC1/KCC2 ratio in these mice.
Our data demonstrate novel mechanisms of interaction between AD and epilepsy and indicate that targeting E:I balance, potentially with US Food and Drug Administration-approved mTOR inhibitors, hold therapeutic promise for AD patients with a seizure history.
Peripherally-derived LGI1-reactive monoclonal antibodies cause epileptic seizures in vivo
Authors: Upadhya Manoj , Kirmann Toni , Wilson Max A , Simon Christian M , Dhangar Divya , Geis Christian , Williams Robyn , Woodhall Gavin , Hallermann Stefan , Irani Sarosh R , Wright Sukhvir K 
Published: April 2024

Abstract
One striking clinical hallmark in patients with autoantibodies to leucine-rich glioma inactivated 1 (LGI1) is the very frequent focal seizure semiologies, including faciobrachial dystonic seizures (FBDS), in addition to the amnesia. Polyclonal serum IgGs have successfully modelled the cognitive changes in vivo but not seizures. Hence, it remains unclear whether LGI1-autoantibodies are sufficient to cause seizures.
We tested this with the molecularly precise monoclonal antibodies directed against LGI1 [LGI1-monoclonal antibodies (mAbs)], derived from patient circulating B cells. These were directed towards both major domains of LGI1, leucine-rich repeat and epitempin repeat, and infused intracerebroventricularly over 7 days into juvenile male Wistar rats using osmotic pumps. Continuous wireless EEG was recorded from a depth electrode placed in hippocampal CA3 plus behavioural tests for memory and hyperexcitability were performed. Following infusion completion (Day 9), post-mortem brain slices were studied for antibody binding and effects on Kv1.1.
The LGI1-mAbs bound most strongly in the hippocampal CA3 region and induced a significant reduction in Kv1.1 cluster number in this subfield. By comparison to control-Ab injected rats video-EEG analysis over 9 days revealed convulsive and non-convulsive seizure activity in rats infused with LGI1-mAbs, with a significant number of ictal events. Memory was not impaired in the novel object recognition test.
Peripherally-derived human LGI1-mAbs infused into rodent CSF provide strong evidence of direct in vivo epileptogenesis with molecular correlations. These findings fulfill criteria for LGI1-antibodies in seizure causation.
A new type of blood–brain barrier aminoacidopathy underlies metabolic microcephaly associated with SLC1A4 mutations
Authors: Odeh Maali , Sajrawi Clara , Majcher Adam , Zubedat Salman , Shaulov Lihi , Radzishevsky Alex , Mizrahi Liron , Chung Wendy K , Avital Avi , Hornemann Thorsten , Liebl Daniel J , Radzishevsky Inna , Wolosker Herman 
Published: April 2024

Abstract
Mutations in the SLC1A4 transporter lead to neurodevelopmental impairments, spastic tetraplegia, thin corpus callosum and microcephaly in children. SLC1A4 catalyses obligatory amino acid exchange between neutral amino acids, but the physiopathology of SLC1A4 disease mutations and progressive microcephaly remain unclear.
Here, we examined the phenotype and metabolic profile of three Slc1a4 mouse models: a constitutive Slc1a4-knockout mouse; a knock-in mouse with the major human Slc1a4 mutation (Slc1a4-K256E); and a selective knockout of Slc1a4 in brain endothelial cells (Slc1a4tie2-cre).
We show that Slc1a4 is a bona fideL-serine transporter at the blood–brain barrier (BBB) and that acute inhibition or deletion of Slc1a4 leads to a decrease in serine influx into the brain. This results in microcephaly associated with decreased L-serine content in the brain, accumulation of atypical and cytotoxic 1-deoxysphingolipids, neurodegeneration, synaptic and mitochondrial abnormalities and behavioural impairments. Prenatal and early postnatal oral administration of L-serine at levels that replenish the serine pool in the brain rescued the observed biochemical and behavioural changes. Administration of L-serine until the second postnatal week also normalized brain weight in Slc1a4-E256K mice.
Our observations suggest that the transport of ‘non-essential’ amino acids from the blood through the BBB is at least as important as that of essential amino acids for brain metabolism and development. We propose that SLC1A4 mutations cause a BBB aminoacidopathy with deficits in serine import across the BBB, required for optimal brain growth, leading to a metabolic microcephaly, which may be amenable to treatment with L-serine.
Exploring the causal role of immune cells in cerebral aneurysm through single-cell transcriptomics and Mendelian randomization analysis
Authors: Yu Yunhu , Tong Shiao , Liu Tao , Cai Yunpeng , Song Yuanmei , Zhou Hang , Jiang Rongcai 
Published: April 2024

Abstract
Cerebral aneurysm (CA) represents a significant clinical challenge, characterized by pathological dilation of cerebral arteries. Recent evidence underscores the crucial involvement of immune cells in CA pathogenesis. This study aims to explore the complex interplay between immune cells and CA formation. We analyzed single-cell RNA sequencing data from the GSE193533 dataset, focusing on unruptured CA and their controls. Comprehensive cell-type identification and pseudo-time trajectory analyses were conducted to delineate the dynamic shifts in immune cell populations. Additionally, a two-sample Mendelian randomization (MR) approach was employed to investigate the causal influence of various immunophenotypes on CA susceptibility and the reciprocal effect of CA formation on immune phenotypes. Single-cell transcriptomic analysis revealed a progressive loss of vascular smooth muscle cells (VSMCs) and an increase in monocytes/macrophages (Mo/MΦ) and other immune cells, signifying a shift from a structural to an inflammatory milieu in CA evolution. MR analysis identified some vital immunophenotypes, such as CD64 on CD14+ CD16+ monocytes (OR: 1.236, 95% CI: 1.064–1.435, P = 0.006), as potential risk factors for CA development, while others, like CD28– CD8br %CD8br (OR: 0.883, 95% CI: 0.789–0.988, P = 0.030), appeared protective. Reverse MR analysis demonstrated that CA formation could modulate specific immunophenotypic expressions, highlighting a complex bidirectional interaction between CA pathology and immune response. This study underscores the pivotal role of immune cells in this process through the integration of single-cell transcriptomics with MR analysis, offering a comprehensive perspective on CA pathogenesis, and potentially guiding future therapeutic strategies targeting specific immune pathways.
A Comparison of Markov and Mechanistic Models for Soil-Transmitted Helminth Prevalence Projections in the Context of Survey Design
Authors: Eyre Max T , Bulstra Caroline A , Johnson Olatunji , de Vlas Sake J , Diggle Peter J , Fronterrè Claudio , Coffeng Luc E 
Published: April 2024

Abstract
Globally, there are over 1 billion people infected with soil-transmitted helminths (STHs), mostly living in marginalized settings with inadequate sanitation in sub-Saharan Africa and Southeast Asia. The World Health Organization recommends an integrated approach to STH morbidity control through improved access to sanitation and hygiene education and the delivery of preventive chemotherapy (PC) to school-age children delivered through schools. Progress of STH control programs is currently estimated using a baseline (pre-PC) school-based prevalence survey and then monitored using periodical school-based prevalence surveys, known as Impact Assessment Surveys (IAS). We investigated whether integrating geostatistical methods with a Markov model or a mechanistic transmission model for projecting prevalence forward in time from baseline can improve IAS design strategies. To do this, we applied these 2 methods to prevalence data collected in Kenya, before evaluating and comparing their performance in accurately informing optimal survey design for a range of IAS sampling designs. We found that, although both approaches performed well, the mechanistic method more accurately projected prevalence over time and provided more accurate information for guiding survey design. Both methods performed less well in areas with persistent STH hotspots where prevalence did not decrease despite multiple rounds of PC. Our findings show that these methods can be useful tools for more efficient and accurate targeting of PC. The general framework built in this paper can also be used for projecting prevalence and informing survey design for other neglected tropical diseases.
An Ensemble Framework for Projecting the Impact of Lymphatic Filariasis Interventions Across Sub-Saharan Africa at a Fine Spatial Scale
Authors: Touloupou Panayiota , Fronterre Claudio , Cano Jorge , Prada Joaquin M , Smith Morgan , Kontoroupis Periklis , Brown Paul , Rivera Rocio Caja , de Vlas Sake J , Gunawardena Sharmini , Irvine Michael A , Njenga Sammy M , Reimer Lisa , Seife Fikre , Sharma Swarnali , Michael Edwin , Stolk Wilma A , Pulan Rachel , Spencer Simon E F , Hollingsworth T Déirdre 
Published: April 2024

Abstract

Background
Lymphatic filariasis (LF) is a neglected tropical disease targeted for elimination as a public health problem by 2030. Although mass treatments have led to huge reductions in LF prevalence, some countries or regions may find it difficult to achieve elimination by 2030 owing to various factors, including local differences in transmission. Subnational projections of intervention impact are a useful tool in understanding these dynamics, but correctly characterizing their uncertainty is challenging.

Methods
We developed a computationally feasible framework for providing subnational projections for LF across 44 sub-Saharan African countries using ensemble models, guided by historical control data, to allow assessment of the role of subnational heterogeneities in global goal achievement. Projected scenarios include ongoing annual treatment from 2018 to 2030, enhanced coverage, and biannual treatment.

Results
Our projections suggest that progress is likely to continue well. However, highly endemic locations currently deploying strategies with the lower World Health Organization recommended coverage (65%) and frequency (annual) are expected to have slow decreases in prevalence. Increasing intervention frequency or coverage can accelerate progress by up to 5 or 6 years, respectively.

Conclusions
While projections based on baseline data have limitations, our methodological advancements provide assessments of potential bottlenecks for the global goals for LF arising from subnational heterogeneities. In particular, areas with high baseline prevalence may face challenges in achieving the 2030 goals, extending the “tail” of interventions. Enhancing intervention frequency and/or coverage will accelerate progress. Our approach facilitates preimplementation assessments of the impact of local interventions and is applicable to other regions and neglected tropical diseases.