bluuebunny/crossref_metadata_2025_split huggingface dataset:
Persistence of high‐risk human papillomavirus infection in a population‐based cohort of Danish women
Authors: Nielsen Ann , Kjaer Susanne K. , Munk Christian , Osler Merete , Iftner Thomas 
Published: February 2010

AbstractPersisting human papillomavirus (HPV) infection is a critical step in cervical carcinogenesis. This study was conducted to determine the type‐specific HPV persistence and risk factors for persistence of high‐risk HPV infections in a large cohort of Danish women. The study was based on a population‐based prospective cohort study of women aged 20–29 years. Participants were interviewed and underwent two gynecological examinations 2 years apart. Women with Hybrid Capture 2 results at enrolment and a follow‐up visit were included in the analysis (n = 7,418). Persistence was defined as positivity for the same high‐risk HPV type at both examinations. Overall, 4.2% of the women had persistent HPV infection, accounting for 26.9% of the initially HPV‐positive women. HPV 16, HPV 58, and HPV 31, all from species group alpha 9, were the most persistent types; however, other high‐risk HPV types that are detected rarely in cancer cases were also likely to persist. The number of high‐risk HPV types and detection of HPV 16 infection at baseline and ever use of oral contraceptives increased the risk for persistence. The risk factor analyses also showed that use of an intrauterine device decreased the risk for persistent high‐risk HPV infection among women with one high‐risk HPV type at baseline. No association was found with viral load or smoking. In conclusion, persistent high‐risk HPV infection, especially HPV 16 persistence, was common among women positive for high‐risk HPV. J. Med. Virol. 82:616–623, 2010. © 2010 Wiley‐Liss, Inc.
Paring and intense pulsed light versus paring alone for recalcitrant hand and foot warts: A randomized clinical trial with blinded outcome evaluation
Authors: Togsverd‐Bo Katrine , Gluud Christian , Winkel Per , Larsen Helle K. , Lomholt Hans B. , Cramers Marie , Bjerring Peter , Hædersdal Merete 
Published: February 2010

AbstractBackground and ObjectivesTreatment of recalcitrant viral warts remains a therapeutic challenge. Intense pulsed light (IPL) has been suggested effective to clear wart tissue. The objective was in a randomized controlled trial to assess the efficacy of paring followed by IPL versus paring alone for recalcitrant hand and foot warts.Materials and MethodsEighty‐nine patients with recalcitrant hand and foot warts were included and randomized (1:1) to three treatments at 3‐week intervals with either paring of warts followed by IPL or paring of warts alone. IPL was given with the Ellipse Flex IPL system (Danish Dermatologic Development A/S, Hørsholm, Denmark, 400–950 nm, 5.5 millisecond pulse duration in double pulses with a 2 millisecond interval, 26.0–32.5 J/cm2 repetitive passes). The primary outcome was complete and partial clearance of warts evaluated by blinded photo assessment at 6 weeks after final treatment. Secondary outcomes were treatment related pain and adverse reactions.ResultsWe found no significant difference in clearance of warts between the two intervention groups (OR 1.64, 95% confidence interval 0.62–4.38). Paring followed by IPL resulted in complete or partial clearance of wart tissue in nine (22%) and five patients (12.2%) versus five (13.5%) and four patients (10.8%) from paring alone. Mostly plantar warts were treated (92.1%). The pain intensity after paring and IPL was moderate and significantly higher than the pain intensity after paring alone (P<0.0005). No adverse reactions were observed from the two interventions.ConclusionParing followed by IPL did not differ significantly from paring alone in clearance of recalcitrant hand and foot warts but caused significantly more pain. Lasers Surg. Med. 42:179–184, 2010. © 2009 Wiley‐Liss, Inc.
Fractional CO2 laser‐assisted drug delivery
Authors: Hædersdal Merete , Sakamoto Fernanda H. , Farinelli William A. , Doukas Apostolos G. , Tam Josh , Anderson R. Rox 
Published: February 2010

AbstractBackground and ObjectivesAblative fractional resurfacing (AFR) creates vertical channels that might assist the delivery of topically applied drugs into skin. The purpose of this study was to evaluate drug delivery by CO2 laser AFR using methyl 5‐aminolevulinate (MAL), a porphyrin precursor, as a test drug.Materials and MethodsTwo Yorkshire swine were treated with single‐hole CO2 laser AFR and subsequent topical application of MAL (Metvix®, Photocure ASA, Oslo, Norway), placebo cream and no drug. MAL‐induced porphyrin fluorescence was measured by fluorescence microscopy at skin depths down to 1,800 µm. AFR was performed with a 10.6 µm wavelength prototype CO2 laser, using stacked single pulses of 3 millisecond and 91.6 mJ per pulse.ResultsAFR created cone‐shaped channels of approximately 300 µm diameter and 1,850 µm depth that were surrounded by a 70 µm thin layer of thermally coagulated dermis. There was no porphyrin fluorescence in placebo cream or untreated skin sites. AFR followed by MAL application enhanced drug delivery with significantly higher porphyrin fluorescence of hair follicles (P<0.0011) and dermis (P<0.0433) versus MAL alone at skin depths of 120, 500, 1,000, 1,500, and 1,800 µm. AFR before MAL application also enhanced skin surface (epidermal) porphyrin fluorescence. Radial diffusion of MAL from the laser‐created channels into surrounding dermis was evidenced by uniform porphyrin fluorescence up to 1,500 µm from the holes (1,000, 1,800 µm depths). Skin massage after MAL application did not affect MAL‐induced porphyrin fluorescence after AFR.ConclusionsAblative fractional laser treatment facilitates delivery of topical MAL deeply into the skin. For the conditions of this study, laser channels approximately 3 mm apart followed by MAL application could produce porphyrins throughout essentially the entire skin. AFR appears to be a clinically practical means for enhancing uptake of MAL, a photodynamic therapy drug, and presumably many other topical skin medications. Lasers Surg. Med. 42:113–122, 2010. © 2009 Wiley‐Liss, Inc.
Long‐term efficacy of a fractional resurfacing device
Authors: Ortiz Arisa E. , Tremaine Anne Marie , Zachary Christopher B. 
Published: February 2010

AbstractBackground and ObjectiveRecently, there has been much debate regarding the long‐term efficacy of fractional resurfacing devices. While pulsed CO2 laser resurfacing is considered a highly effective treatment, fractionated resurfacing is a newer modality and its long‐term efficacy has yet to be assessed. We report the long‐term outcomes of subjects previously treated with fractional CO2 resurfacing for photodamaged skin and acne scars.Study Design/Materials and MethodsTen subjects from our previous studies who received fractional resurfacing for the treatment of acne scarring and photodamage returned for long‐term follow‐up visits at 1 and 2 years, respectively. Investigators graded maintenance of improvement on a quartile scale based on clinical photography.ResultsSubjects maintained 74% of their overall improvement at their long‐term visits compared to 3‐month follow‐up visits. While clinical improvement was maintained long‐term, the results were not as remarkable as those seen at 3‐month visits. The authors speculate that results seen at 3 months may be enhanced by persistent inflammatory changes, as evidenced by heat shock protein 47 activity and ongoing collagen remodeling seen in previous histologic studies. Relaxation of tightening is to be expected with any procedure along with the natural progression of aging. However, patient satisfaction was upheld long‐term.ConclusionFractional CO2 laser resurfacing does have long‐term efficacy and persistence of improvement of acne scarring and photodamage compared to baseline. However, additional treatments may be necessary to enhance long‐term results. Lasers Surg. Med. 42:168–170, 2010. © 2010 Wiley‐Liss, Inc.
Long‐term blood vessel removal with combined laser and topical rapamycin antiangiogenic therapy: Implications for effective port wine stain treatment
Authors: Jia Wangcun , Sun Victor , Tran Nadia , Choi Bernard , Liu Shaiw‐wen , Mihm Martin C. , Phung Thuy L. , Nelson J. Stuart 
Published: February 2010

AbstractBackground and ObjectivesComplete blanching of port wine stain (PWS) birthmarks after laser therapy is rarely achieved for most patients. We postulate that the low therapeutic efficacy or treatment failure is caused by regeneration and revascularization of photocoagulated blood vessels due to angiogenesis associated with the skin's normal wound healing response. Rapamycin (RPM), an antiangiogenic agent, has been demonstrated to inhibit growth of pathological blood vessels. Our objectives were to (1) investigate whether topical RPM can inhibit reperfusion of photocoagulated blood vessels in an animal model and (2) determine the effective RPM concentration required to achieve this objective.Study Design/Materials and MethodsFor both laser‐only and combined laser and RPM treated animals, blood vessels in the dorsal window chambers implanted on golden Syrian hamsters were photocoagulated with laser pulses. Structural and flow dynamics of blood vessels were documented with color digital photography and laser speckle imaging to evaluate photocoagulation and reperfusion. For the combined treatment group, topical RPM was applied to the epidermal side of the window daily for 14 days after laser exposure.ResultsIn the laser‐only group, 23 out of 24 photocoagulated blood vessels reperfused within 5–14 days. In the combined treatment group with different RPM formulae and concentrations, the overall reperfusion rate of 36% was much lower as compared to the laser‐only group. We also found that the reperfusion rate was not linearly proportional to the RPM concentration.ConclusionsWith topical RPM application, the frequency of vessel reperfusion was considerably reduced, which implies that combined light and topical antiangiogenic therapy might be a promising approach to improve the treatment efficacy of PWS birthmarks. Lasers Surg. Med. 42:105–112, 2010. © 2010 Wiley‐Liss, Inc.
Sulforaphane protects cortical neurons against 5‐S‐cysteinyl‐dopamine‐induced toxicity through the activation of ERK1/2, Nrf‐2 and the upregulation of detoxification enzymes
Authors: Vauzour David , Buonfiglio Maria , Corona Giulia , Chirafisi Joselita , Vafeiadou Katerina , Angeloni Cristina , Hrelia Silvana , Hrelia Patrizia , Spencer Jeremy P. E. 
Published: February 2010

AbstractThe degeneration of dopaminergic neurons in the substantia nigra has been linked to the formation of the endogenous neurotoxin 5‐S‐cysteinyl‐dopamine. Sulforaphane (SFN), an isothiocyanate derived from the corresponding precursor glucosinolate found in cruciferous vegetables has been observed to exert a range of biological activities in various cell populations. In this study, we show that SFN protects primary cortical neurons against 5‐S‐cysteinyl‐dopamine induced neuronal injury. Pre‐treatment of cortical neurons with SFN (0.01–1 μM) resulted in protection against 5‐S‐cysteinyl‐dopamine‐induced neurotoxicity, which peaked at 100 nM. This protection was observed to be mediated by the ability of SFN to modulate the extracellular signal‐regulated kinase 1 and 2 and the activation of Kelch‐like ECH‐associated protein 1/NF‐E2‐related factor‐2 leading to the increased expression and activity of glutathione‐S‐transferase (M1, M3 and M5), glutathione reductase, thioredoxin reductase and NAD(P)H oxidoreductase 1. These data suggest that SFN stimulates the NF‐E2‐related factor‐2 pathway of antioxidant gene expression in neurons and may protect against neuronal injury relevant to the aetiology of Parkinson's disease.
Vitamin E transport, membrane incorporation and cell metabolism: Is α‐tocopherol in lipid rafts an oar in the lifeboat?
Authors: Lemaire‐Ewing Stéphanie , Desrumaux Catherine , Néel Dominique , Lagrost Laurent 
Published: February 2010

AbstractVitamin E is composed of closely related compounds, including tocopherols and tocotrienols. Studies of the last decade provide strong support for a specific role of α‐tocopherol in cell signalling and the regulation of gene expression. It produces significant effects on inflammation, cell proliferation and apoptosis that are not shared by other vitamin E isomers with similar antioxidant properties. The different behaviours of vitamin E isomers might relate, at least in part, to the specific effects they exert at the plasma membrane. α‐Tocopherol is not randomly distributed throughout the phospholipid bilayer of biological membranes, and as compared with other isomers, it shows a propensity to associate with lipid rafts. Distinct aspects of vitamin E transport and metabolism is discussed with emphasis on the interaction between α‐tocopherol and lipid rafts and the consequences of these interactions on cell metabolism.
Square and stretch multigrid for stochastic matrix eigenproblems
Authors: Treister Eran , Yavneh Irad 
Published: February 2010

AbstractA novel multigrid algorithm for computing the principal eigenvector of column‐stochastic matrices is developed. The method is based on an approach originally introduced by Horton and Leutenegger (Perform. Eval. Rev. 1994; 22:191–200) whereby the coarse‐grid problem is adapted to yield a better and better coarse representation of the original problem. A special feature of the present approach is the squaring of the stochastic matrix—followed by a stretching of its spectrum—just prior to the coarse‐grid correction process. This procedure is shown to yield good convergence properties, even though a cheap and simple aggregation is used for the restriction and prolongation matrices, which is important for maintaining competitive computational costs. A second special feature is a bottom–up procedure for defining coarse‐grid aggregates. Copyright © 2010 John Wiley & Sons, Ltd.
On‐plate enrichment of glycopeptides by using boronic acid functionalized gold‐coated Si wafer
Authors: Xu Yawei , Zhang Lijuan , Lu Haojie , Yang Pengyuan 
Published: February 2010

AbstractBoronic acid functionalized gold‐coated Si wafer has been used as MALDI plate to isolate and enrich glycopeptides for MS analysis. This on‐plate enrichment strategy offers good benefits due to the combination of specific selectivity through enrichment and direct manipulation on the wafer. First, solution transfer and eluting steps required in conventional enrichment strategies are not needed any more, thereby reducing sample loss during these steps. Second, the LODs of glycopeptides have been increased by two orders of magnitude. Third, non‐specific bindings have not been detected even when non‐glycopeptides are 100 times more than glycopeptides. Furthermore, the recovery of glycopeptide is up to 65.8% and glycopeptides even can be sensitively detected in the presence of 200 mM ammonium bicarbonate or physiological buffer PBS.
ELL is an HIF‐1α partner that regulates and responds to hypoxia response in PC3 cells
Authors: Liu Lingqi , Ai Junkui , Xiao Wuhan , Liu June , Wang Yujuan , Xin Dianqi , He Zhisong , Guo Yinglu , Wang Zhou 
Published: February 2010

AbstractBACKGROUNDEleven–nineteen lysine‐rich leukemia (ELL) plays an important role in tumorigenesis and animal development. HIF‐1 is a transcriptional factor that functions as a master regulator of O2 homeostasis. Our previous studies showed that a binding partner of ELL, U19/Eaf2, can modulate HIF‐1α activity and hypoxia response, suggesting that ELL may also influence HIF‐1α pathway and hypoxia response.METHODSCo‐localization and co‐immunoprecipitation were performed to test the interaction between ELL and HIF‐1α. PC3 cells with stable ELL knockdown and PC3 cells with stable ELL overexpression, along with their controls, were established using lentiviral expression system. Western blot and real‐time PCR were performed to test the effect of ELL on HIF‐1α protein and its down‐stream gene transcription. To elucidate potential effect of hypoxia on ELL, cell growth and colony formation assays were performed using PC3 subline with stable ELL overexpression.RESULTSELL is associated with HIF‐1α in transfected cells. In PC3 prostate cancer cells, ELL inhibited HIF‐1α protein level and down‐stream gene expression. As expected, ELL inhibited cell growth and colony formation under normoxia. Interestingly, the inhibition was alleviated under hypoxia.CONCLUSIONSOur findings suggest that ELL and HIF‐1α are binding partners and can modulate the functions of each other in hypoxia. Prostate 70: 797–805, 2010. © 2010 Wiley‐Liss, Inc.
Androgens, growth factors, and risk of prostate cancer: The Multiethnic Cohort
Authors: Gill Jasmeet K. , Wilkens Lynne R. , Pollak Michael N. , Stanczyk Frank Z. , Kolonel Laurence N. 
Published: February 2010

AbstractBACKGROUNDAndrogens and growth factors are thought to be associated with prostate cancer risk, although past research has produced mixed results.METHODSWe conducted a nested case–control study of biomarkers of prostate cancer risk within the Multiethnic Cohort. We compared prediagnostic levels of testosterone, dihydrotestosterone (DHT), sex hormone‐binding globulin (SHBG), 3α‐androstanediol glucuronide (3α‐diol G), insulin‐like growth factor I (IGF‐I), IGF‐II, IGF‐binding protein 1 (IGFBP‐1), and IGFBP‐3 in serum from 467 incident prostate cancer cases and 934 cancer‐free controls. Controls were matched to the cases on geographic site (HI, LA), ethnicity, age at specimen collection (±1 year), date (±1 month) and time of day (±2 hr) of sample collection, and fasting status (<6, 6–7, 8–9, >10 hr). Multivariate conditional logistic regression models were used to compute adjusted odds ratios (ORs) and 95% confidence intervals (CIs).RESULTSSerum concentrations of testosterone, DHT, SHBG, 3α‐diol G, IGF‐I, IGF‐II, IGFBP‐1, and IGFBP‐3 were not associated with risk of prostate cancer. Tests for trend of quartiles of serum concentrations also did not show any association. Results were relatively unchanged for men with advanced prostate cancer and their matched controls. However, the follow‐up period was relatively short (mean of 1.9 years). Analysis by ethnic group showed an increased risk for Latino men in the second (OR = 3.67, 95% CI: 1.63–8.24) and third (OR = 2.96, 95% CI: 1.19–7.40) tertiles of IGF‐I serum levels compared with the first tertile.CONCLUSIONSThe suggested increased risk for IGF‐I in Latino men merits further study, with greater statistical power. Prostate 70: 906–915, 2010. © 2010 Wiley‐Liss, Inc.
Inhibition of DHCR24/Seladin‐1 impairs cellular homeostasis in prostate cancer
Authors: Battista Marie‐Claude , Guimond Marie‐Odile , Roberge Claude , Doueik Alexandre A. , Fazli Ladan , Gleave Martin , Sabbagh Robert , Gallo‐Payet Nicole 
Published: February 2010

AbstractBACKGROUNDSeladin‐1 belongs to a subgroup of androgen‐dependent genes associated with anti‐proliferative, pro‐differentiation, and pro‐apoptotic functions and plays a protective role against oncogenic stress. The present study aims to investigate the localization and expression of Seladin‐1 protein in normal and tumoral human prostatic tissues as well as to explore its role in proliferation and steroid secretion in androgen‐dependent (LnCaP) and androgen‐independent (DU145) cell lines and in human prostate primary cell culture.METHODSSeladin‐1 protein localization and expression were assessed on whole tissue sections by tissue array/immunohistochemistry and following immunofluorescence and Western blotting. Proliferation (Ki67 fluorescence labeling and cell counts) and steroid secretion (ELISA) were assessed in cell lines and primary epithelial cell cultures.RESULTSIn human prostatic tissue and cells, Seladin‐1 was mostly localized within epithelial and rarely within stromal cells and primarily present in secretory luminal cells of normal and tumoral prostate cells. Its expression was increased in low‐risk prostate cancer but reduced in advanced prostate cancers when compared to normal tissues. Seladin‐1 was highly expressed in LnCaP, whereas its expression level was lower in DU145 cells. Seladin‐1 inhibition by treatment with its specific inhibitor, U18666A (75 nM), increased proliferation in LnCaP and primary cell culture, as well as testosterone and dihydrotestosterone levels in both LnCaP and DU145 cell lines.CONCLUSIONSSeladin‐1 involvement in proliferation and secretion suggests that its downregulation may be a major mechanism causing prostate cancer evolution. Seladin‐1 may thus potentially decrease cell growth and steroid dependency in low‐grade prostate cancer. Prostate 70: 921–933, 2010. © 2010 Wiley‐Liss, Inc.
The Prostate Cancer Foundation 16th Annual Scientific Retreat: Meeting report
Authors: Rhodes Katrin E. , Simons Jonathan W. , Soule Howard R. 
Published: February 2010

AbstractThe 16th Annual Prostate Cancer Foundation (PCF) Scientific Retreat was held September 23‐25, 2009 at Incline Village, NV. This report highlights some of the game‐changing research reports from this conference. Prostate 70: 916–920, 2010. © 2010 Wiley‐Liss, Inc.
Differential expression of the α2 chain of the interleukin‐13 receptor in metastatic human prostate cancer ARCaPM cells.
Authors: He Hui , Xu Jianchun , Nelson Peter S. , Marshall Fray F. , Chung Leland W.K. , Zhau Haiyen E. , He Dalin , Wang Ruoxiang 
Published: February 2010

AbstractBACKGROUNDThe α2 chain of the interleukin‐13 receptor (IL13Rα2) is a high‐affinity receptor and a candidate target for cytotoxic killing of cancer cells. Availability of a human prostate cancer cell line with high level of IL13Rα2 expression will facilitate the development of therapeutic modalities.METHODSARCaPE and ARCaPM human prostate cancer cell lines were subjected to comparative analyses of gene expression. Expression of the IL13Rα2 protein was confirmed by Western blotting and immunostaining. IL13Rα2 proteins in xenograft tumors and clinical human prostate cancer specimens were detected by specific antibodies. LNCaP prostate cancer cells stably transfected with IL13Rα2 were examined for accelerated growth in athymic mice.RESULTSWe found that IL13Rα2 proteins could be detected in both the ARCaPE and ARCaPM cells, but the expression level in ARCaPM was more than 17‐fold higher than in ARCaPE cells. Importantly, the ARCaP lineage represented the only human prostate cancer cell line that expresses IL13Rα2 proteins at the level detectable by Western blotting. Expression of IL13Rα2 was accompanied by resistance to the anti‐tumor activity of interleukin‐13 (IL‐13). ARCaP cells were found to be insensitive to growth inhibition upon IL‐13 treatment, while overexpression of IL13Rα2 in LNCaP cells promoted intratibial tumor growth in athymic mice.CONCLUSIONSDifferential IL13Rα2 expression may account for the high tumorigenic and metastatic potential of ARCaPM cells. The unique expression of IL13Rα2 makes ARCaP lineage an attractive model for evaluating the targeting efficacy of therapeutic agents based on IL13Rα2 protein expression. Prostate © 2010 Wiley‐Liss, Inc.
The crossed‐field and single‐field Hall effect in LuRh2Si2
Authors: Friedemann Sven , Oeschler Niels , Wirth Steffen , Steglich Frank , MaQuilon Sam , Fisk Zachary 
Published: February 2010

AbstractThe Hall effect of LuRh2Si2 – the non‐magnetic homologue of the heavy‐fermion material YbRh2Si2 – is studied with two different setups: in the conventional single‐field geometry, the field dependence is analyzed in terms of the differential Hall coefficient. Beyond that, the recently developed crossed‐field experiment allows to examine the linear‐response Hall coefficient as a function of magnetic field. The results reveal the expected analogy between both experiments which corroborates the equivalent findings in YbRh2Si2. This emphasizes the feasibility to investigate field‐induced quantum critical points with both methods.Setup of the crossed‐field Hall‐effect experiment allows to examine the linear‐response (with respect to the magnetic field B1) Hall coefficient as a function of tuning field B2.magnified image