bluuebunny/crossref_metadata_2025_split huggingface dataset:
E2F‐1‐ and E2Ftr‐mediated apoptosis: the role of DREAM and HRK
Authors: Hao Hongying , Chen Canming , Rao Xiao‐Mei , Gomez‐Gutierrez Jorge G. , Zhou H. Sam , McMasters Kelly M. 
Published: May 2011

AbstractE2F‐1‐deleted mutant, ‘truncated E2F’ (E2Ftr, E2F‐1[1–375]), lacking the carboxy‐terminal transactivation domain, was shown to be more potent at inducing cancer cell apoptosis than wild‐type E2F‐1 (wtE2F‐1; full‐length E2F‐1). Mechanisms by which wtE2F‐1 and E2Ftr induce apoptosis, however, are not fully elucidated. Our study demonstrates molecular effects of pro‐apoptotic BH3‐only Bcl‐2 family member Harakiri (Hrk) in wtE2F‐1‐ and E2Ftr‐induced melanoma cell apoptosis. We found that Hrk mRNA and Harakiri (HRK) protein expression was highly up‐regulated in melanoma cells in response to wtE2F‐1 and E2Ftr overexpression. HRK up‐regulation did not require the E2F‐1 transactivation domain. In addition, Hrk gene up‐regulation and HRK protein expression did not require p53 in cancer cells. Hrk knockdown by Hrk siRNA was associated with significantly reduced wtE2F‐1‐ and E2Ftr‐induced apoptosis. We also found that an upstream factor, ‘downstream regulatory element antagonist modulator’ (DREAM), may be involved in HRK‐mediated apoptosis in response to wtE2F‐1 and E2Ftr overexpression. DREAM expression levels increased following wtE2F‐1 and E2Ftr overexpression. Western blotting detected increased DREAM primarily in dimeric form. The homodimerization of DREAM resulting from wtE2F‐1 and E2Ftr overexpression may contribute to the decreased binding activity of DREAM to the 3′‐untranslated region of the Hrk gene as shown by electromobility shift assay. Results showed wtE2F‐1‐ and E2Ftr‐induced apoptosis is partially mediated by HRK. HRK function is regulated in response to DREAM. Our findings contribute to understanding the mechanisms that regulate wtE2F‐1‐ and E2Ftr‐induced apoptosis and provide insights into the further evaluation of how E2Ftr‐induced apoptosis may be used for therapeutic gain.
Maternal and perinatal complications by day of gestation after spontaneous labor at 40–42 weeks of gestation
Authors: GREVE TINE , LUNDBYE‐CHRISTENSEN SØREN , NICKELSEN CARSTEN N. , SECHER NIELS J. 
Published: May 2011

Abstract Objective. To evaluate pregnancy outcome after spontaneous labor by day of gestation between 40+0 and 41+6 weeks of gestation. Design. Evaluation of prospectively collected labor ward data. Setting. University Hospital, Denmark. Population. Unselected consecutive cohort of 14 678 spontaneously starting deliveries between 280 and 293 days of gestation during the years 2000–2006. Methods. Data were registered in a computer program after each delivery by a midwife and the entries further evaluated by a specialist in obstetrics. Complication rates were compared using Fisher's exact test. Main Outcome Measures. Maternal complication rates for each gestation day, including cesarean delivery, maternal blood transfusion, episiotomy, operative vaginal delivery, third and fourth degree perineal lacerations and perinatal morbidity. Results. The cesarean delivery rate increased from 6% on day 280 to 11% on day 293. Cesarean delivery increased from 40 to 41 weeks gestation (7.3 vs. 9.5%, p<0.005), as did maternal transfusion (0.5 vs. 1.2%, p<0.001) and cesarean section on the indication fetal distress (1.5 versus 2.4%, p<0.005), but perineal lacerations did not. Likewise, there was an increase in episiotomy rates (3.0 vs. 3.5%, p=0.08), operative vaginal delivery (5.8 vs. 6.5%, p=0.07) and admission to neonatal intensive care (1.4 versus 2.0%, p=0.009), but no increase in 5 minute Apgar scores <7 or low umbilical artery acid–base values. Conclusions. Deliveries starting spontaneously in an unselected cohort showed an increase in maternal complications, meconium‐stained amniotic fluid and admission to the neonatal intensive care unit.
Cord blood collection for banking and the risk of maternal hemorrhage
Authors: AMAT LLUÍS , SABRIÀ JOAN , MARTÍNEZ EVA , RODRÍGUEZ NÚRIA L. , QUEROL SERGIO , LAILLA JOSEP M. 
Published: May 2011

AbstractWe determined the effect of cord blood collection before placental expulsion on postpartum maternal blood loss in a retrospective study between a group of cord blood donors and a group of non‐donors. The study was conducted in a university hospital blood bank and obstetric services and included Spanish women entered in a European study project (EUPHRATES) and who had consented to donate cord blood for public banking purposes. We measured blood volume lost during delivery by a bag collection method, as well as the need for transfusion and postpartum anemia symptoms. Deliveries at which cord blood was collected presented a significant increase in blood loss (321±273 vs. 255±237ml, p=0.02). Instrumental deliveries were associated with higher postpartum blood loss than spontaneous deliveries. Cord blood collection can increase intrapartum blood loss, especially at instrumental deliveries. Additional staff who handle the collection are required to allow the leading clinician to focus on maternal care.
The experimental research on two‐generation BLB dental implants ‐ Part I: surface modification and osseointegration
Authors: Yin Kaifeng , Wang Zhifeng , Fan Xin , Bian Yuanyuan , Guo Jing , Lan Jing 
Published: May 2011

AbstractObjective: The study was designed to evaluate the comparative effect of osseointegration induced by the dental implants of Beijing Leiden Biomaterial (BLB) and BLBIII.Materials and methods: The surface properties ofBLBI and BLBIII were studied through thermal field‐scanning electron microscopy, energy‐dispersive X‐ray spectroscopy (EDS) and optical profilometer. A total of 36 BLBI and BLBIII implants, with each of the two groups possessing 18, were randomly implanted into the extraction fossa of the mandibular premolar areas of six Beagles. The animals were then executed 2, 4 and 8 weeks after the surgery, which was followed by macroscopic examination and histomorphometric analysis.Results: Typical cloud‐form microstructure was found on the surface of BLBI implant, which was distributed widely yet in an irregular way. The surface of BLBIII implant was mainly featured by a highly porous layer. The EDS spectra of BLBI indicated the peaks of calcium (Ca) and phosphorous (P) compatible with apatite phase, while the peaks of Ca, P, oxygen and titanium were incorporated in the BLBIII group. The ratio of Ca and K showed no significant differences in the surface chemical composition of BLBI and BLBIII. Surface microtopographic analysis showed a statistical difference (P<0.01) in the roughness between BLBI (Ra) and BLBIII. In the healing period, a larger amount of osteoid and bone tissues were observed in the areas surrounding the BLBIII group than those of the BLBII group. After 2 and 4 weeks of the surgery, the bone–implant contact (BIC) of BLBIII group presented higher value of statistical significance (P<0.05) than that of BLBI. However, after the 8‐week healing period, the BIC difference between the two groups proved to be of no statistical significance (P>0.05).Conclusion: Micro‐arc oxidation (MAO) and electrophoresis deposition (EPD) are able to produce a highly porous layer on the surface of BLBIII, which is characterized by a relatively stable Ca/P ratio similar to that of the hydroxyapatite layer. Therefore, superior and early osseointegration potential was demonstrated in the threaded implants treated by MAO coupling with EPD, rather than the merely cylindrical‐shaped ones with plasma‐sprayed HA coating layer.To cite this article: Yin K, Wang Z, Fan X, Bian Y, Guo J, Lan J. The experimental research on two‐generation BLB dental implants. Part I: surface modification and osseointegration. Clin. Oral Impl. Res. 23, 2012; 846–852. doi: 10.1111/j.1600‐0501.2011.02209.x
Impact of collagen crosslinking on the second harmonic generation signal and the fluorescence lifetime of collagen autofluorescence
Authors: Lutz Vivien , Sattler Martin , Gallinat Stefan , Wenck Horst , Poertner Ralf , Fischer Frank 
Published: May 2011

Background/purpose: Collagen is the major structural protein of the skin and its crosslinks are essential for its mechanical stability. In photodamaged skin, a decrease of the mature collagen crosslink histidinohydroxylysino‐norleucine was reported. In this study, we investigated the consequences and measurability of the reduced crosslinking.Methods: In order to determine the consequences of reduced collagen crosslinking, in vitro models of reduced collagen crosslinking were established. The collagen synthesis and structure was analyzed using the signals second harmonic generation (SHG) and the fluorescence lifetime of the collagen autofluorescence by a multiphoton laser scanning microscope.Results: Reduced collagen crosslinking results in a posttranscriptionally diminished collagen synthesis, a modified structure of the collagen fibers and fibrils and a higher intensity of the SHG signal. The SHG signal might be influenced by the interspaces of the collagen molecules within one collagen fibril. Because of these findings, it can be speculated that reduced collagen crosslinking changes the interspace of single collagen molecules within the collagen fibril, resulting in an enhanced SHG signal. Alternative explanations are discussed. Furthermore, the fluorescence lifetime was reduced in the in vitro models of reduced collagen crosslinking. In the crosslink sites of the collagen molecules, the main ratio of fluorescence is found. As the fluorescence lifetime is determined not only by the fluorescent molecule itself but also by its microenvironment, the change in the fluorescence lifetime might be explained by reduced crosslinking at the crosslink site.Conclusion: A reduction of collagen crosslinking (as seen in photodamaged skin) results in an increase of the SHG signal and a decrease of the fluorescence lifetime in vitro. In vivo measurements of the two parameters might reveal the status of collagen crosslinking and therefore help to identify the status of dermal photodamage or pathogenesis using collagen crosslinking determination.
Microarray analysis of sexually dimorphic gene expression in human minor salivary glands
Authors: Michael D , Soi S , Cabera‐Perez J , Weller M , Alexander S , Alevizos I , Illei GG , Chiorini JA 
Published: May 2011

Oral Diseases (2011) 17, 653–661Objective:  We hypothesized that differential mRNA transcription between the sexes may be linked to the 9:1 female‐to‐male gender‐related relative risk for the development of Sjögren’s syndrome (SS), an autoimmune disease that leads to inflammation and dysfunction in the lachrymal and salivary glands.Subjects and Methods:  RNA from minor salivary glands was collected from nine healthy volunteers (four men and five women) and analyzed using the Agilent 4 × 44K human microarray platform. Differential expression was confirmed by qRT‐PCR.Results:  Comparison of the transcriptome of minor salivary glands from normal male and female volunteers with that of salivary glands and secretory epithelia identified a number of gender, species, and tissue‐specific gene expression patterns. These differences include, but are not limited to, a diverse set of genes involved in immune modulation, chemotactic control, inhibition of complement, metabolism, and neurogenesis.Conclusion:  Analysis of these changes provides insight into the protective and predisposing molecular factors that may be involved in the development of Sjögren’s syndrome. Some of the gene changes observed in this study correlate with previously observed sexual dimorphisms in salivary gland function and also illustrate several new targets for further investigation.
A comparison of two questionnaires measuring oral health‐related quality of life before and after dental hygiene treatment in patients with periodontal disease
Authors: Öhrn K , Jönsson B 
Published: May 2011

To cite this article:

Int J Dent Hygiene
10, 2012; 9–14  DOI: 10.1111/j.1601‐5037.2011.00511.x  Öhrn K, Jönsson B. A comparison of two questionnaires measuring oral health‐related quality of life before and after dental hygiene treatment in patients with periodontal disease.Abstract:  Aim: The aim of this study was to compare the usefulness of two different questionnaires assessing oral health‐related quality of life (OHRQoL) at the basic examination and after initial dental hygiene treatment (DHtx). Methods: A total of 42 patients referred for periodontal treatment completed the Oral Health Impact Profile (OHIP‐14) and the General Oral Health Assessment Index (GOHAI) at the basic periodontal examination. They underwent DHtx and completed the questionnaires once again after the treatment. Results: No statistically significant differences could be found between the two assessments, neither for the total scores nor for any of the separate items of the OHIP‐14 or the GOHAI. However, the GOHAI questionnaire seems to result in a greater variety in the responses indicating that the floor effect is not as pronounced as for the OHIP‐14. Those who had rated their oral health as good reported significantly better OHRQoL on both questionnaires. The same pattern was found for patients who reported that they were satisfied with their teeth. After DHtx and necessary extractions, there was a statistically significant correlation between the number of teeth and the total scores on both questionnaires. No other statistically significant correlations with periodontal variables could be found. Conclusion: No statistically significant difference could be found after DHtx compared to before in regard to OHRQoL assessed with OHIP‐14 and GOHAI. However, there was a greater variety in the responses with the GOHAI questionnaire; it may hereby be more useful for patients with periodontal disease.
CARPE: a registry project of the German Dermatological Society (DDG) for the characterization and care of chronic hand eczema
Authors: Apfelbacher Christian Joachim , Akst Wilhelm , Molin Sonja , Schmitt Jochen , Bauer Andrea , Weisshaar Elke , Mahler Vera , Treichel Sabine , Ruzicka Thomas , Luger Thomas , Elsner Peter , Diepgen Thomas Ludwig 
Published: May 2011

SummaryBackground: The aim of the CARPE (German acronym: Chronisches Handekzem‐Register zum Patienten‐Langzeitmanagement; meaning: chronic hand eczema registry on long‐term patient management) registry is to investigate characteristics and treatment modalities in patients affected by chronic hand eczema in Germany.Methods: The registry was built up under the auspices of the German Dermatological Society (Deutsche Dermatologische Gesellschaft, DDG). Patients with chronic hand eczema are prospectively assessed by dermatolog‐ical examination and patient questionnaire. Socio‐economic data and data on diagnostics, skin status, severity and treatment of chronic hand eczema and atopy criteria are repeatedly assessed. Here, we present baseline characteristics of the first 515 patients.Results: 53.8 % of the patients were female, mean age was 47.3 years. The average duration of CHE was 7.7 years. 30.4 % had already received inpatient care, 31.2 % had been on sick leave in the past 12 months. 94.5 % had received topical corticosteroids prior to inclusion in the registry, 31.9 % topical calcineurin inhibitors, 38.3 % UV therapy, 28.6 % systemic antihistamines, 36.5 % systemic treatments, 14.9 % systemic corticosteroids, 25.8 % systemic retinoids.Conclusions: The CARPE project demonstrates the high medical burden and therapeutic challenge of chronic hand eczema and presents first data for health care research. Furthermore, the designed follow‐up study will present important data about the natural history and prognosis of this chronic skin disease.
Muscle function assessment in children
Authors: Jones MA , Stratton G. 
Published: May 2011

Assessment of muscle function has a range of applications, including appraisal of the presence and severity of muscle weakness and fatigability, examination of the effectiveness of intervention programmes and development and monitoring of training programmes. Muscle function assessment is routinely performed in rehabilitation, physical education, coaching and research. Procedures have been extensively examined in adults, but not in children. The aim of this review is to bring to the forefront the issue of paediatric muscle function assessment. Objective methods of muscle function assessment are critically analysed with specific attention to the issues of applicability, validity and reliability. Each of the methods reviewed (isokinetic dynamometry, hand‐held dynamometry, field tests, and standard weights equipment) has its limitations. All procedures have been occasionally studied for validity and reliability and optimal procedures have yet to be identified. Methodological limits can be generalized to the type and speed of activation, standardization of procedures, subject familiarization, gravity and size and psychological influences. It is important for administrators of muscle function tests to be aware of the various factors that can both influence and confound interpretation.It was concluded that muscle function assessment in children requires significant further investigation. Although there is a wide range of procedures available, there is limited and equivocal research proving validity and reliability. Widespread use of the most valid and reliable methods is encouraged. Future research directions are also considered.
Silent acetaminophen‐induced hepatotoxicity in febrile children: Does this entity exist?
Authors: Shaoul R. , Novikov J. , Maor I. , Jaffe M. 
Published: May 2011

Several descriptions of acetaminophen‐associated liver injury caused by therapeutic or a dosage slightly above the recommended dosage have been described. Our hypothesis is that in sick febrile infants and children, who may also be calorie depleted, there might be an increased hepatic vulnerability to acetaminophen. Aim: (1) To correlate serum acetaminophen levels in febrile infants and children with the following parameters: aspartate aminotransferase (AST) levels, fever, vomiting and/or decreased caloric intake; and (2) to assess parental knowledge regarding the medication dosage and hazards of acetaminophen. Methods: Healthy children with an acute febrile illness, who had received acetaminophen, were eligible to participate in the study. AST and acetaminophen levels were drawn, and a detailed questionnaire was completed for every child. Results: 107 children participated in the study; 50 girls and 57 boys with ages ranging from 1 mo to 16 y (mean 33 mo). All serum acetaminophen levels were within the safety range. Although 32% of parents administered a single acetaminophen dose above 15 mg/kg and 46% gave a daily dose above 60 mg/kg/d, no significant differences were observed in the serum acetaminophen and AST levels compared to those who received the appropriate dose. In about 60% of cases, the high doses were recommended by a physician. Young age and high fever were associated with significantly higher acetaminophen levels. We could not find an association between acetaminophen levels and vomiting, decreased caloric intake and AST levels. Only 24 parents (22%) were aware of the possible toxicity of acetaminophen. Conclusions: No evidence of increased hepatic vulnerability to acetaminophen was noted in a cohort of febrile infants and children. Furthermore, significant numbers of parents and physicians were unaware of acetaminophen dangers.
Oral health in institutionalised elderly people in Oslo, Norway and its relationship with dependence and cognitive impairment
Authors: Zuluaga Dairo J Marín , Ferreira Jenny , Montoya José A. Gil , Willumsen Tiril 
Published: May 2011

doi: 10.1111/j.1741‐2358.2011.00490.x

Oral health in institutionalised elderly people in Oslo, Norway and its relationship with dependence and cognitive impairmentObjective:  Investigating oral health’s relationship with dependency and cognitive state.Background:  Oral hygiene is poor in the institutionalised elderly. There are problems regarding the oral care of residents having poor mobility or cognitive impairment.Material and methods:  Cross‐sectional study involving 135 participants (mean age 85.7, SD 8.8 years) in two categories: nurses doing tooth cleaning and residents doing tooth cleaning. Those cleaned by nurses were categorised as co‐operative or unco‐operative. The oral hygiene status, presence of caries, retained roots and denture‐related stomatitis were recorded.Results:  Of the participants, 70% had only natural teeth. The prevalence of caries was 28%. A significant correlation showed that having more teeth gave a poorer Simplified Oral Hygiene Index (OHI‐S) (p = 0.018). The number of retained roots increased with the severity of cognitive impairment (p < 0.05). Significant differences were found between nurses or residents doing the tooth cleaning on the OHI‐S (p = 0.05) and percentage of dental plaque (p = 0.003). Unco‐operative residents had poorer oral hygiene (p = 0.028), more caries (p = 0.008) and were more often moderate–severe cognitive impaired (p = 0.016).Conclusions:  A high percentage of participants had unacceptable oral hygiene. Residents whose teeth were cleaned by the nurses had poorer oral hygiene. Unco‐operative residents had the worst oral hygiene and more caries.
Assessing oral health promotion determinants in active Greek elderly
Authors: Naka Olga , Anastassiadou Vassiliki 
Published: May 2011

doi: 10.1111/j.1741‐2358.2011.00491.x

Assessing oral health promotion determinants in active Greek elderlyObjective:  To explore older adults’ patterns and risk behaviours to be invoked in the allocation of strategies to promote oral health.Background:  Access to dental services is determined by factors that serve as barriers to or enablers of older adults’ behaviour and attitudes towards oral health. Appropriate oral health promotion activities are of particular importance among the elderly in altering oral health behaviour through education, prevention and health protection.Methods:  A cross‐sectional study was conducted among 108 dentate elderly subjects. They were attending a University Prosthetic Dentistry Clinic in Greece as patients seeking treatment. Participants underwent a clinical examination and an interview.Results:  Data extracted revealed that cost and no disease awareness were the most frequently mentioned barriers to regular dental visits. Most of the participants presented low level of income and education and reduced presence of adverse dental health symptoms compared with the actual oral health status. Besides some predisposing factors, enabling and need variables significantly affected access to dental care services, participants’ number of remaining teeth and as a consequence oral health status.Conclusion:  Profiling older adults’ demographics, economic, social and cultural status and their attitudes and beliefs could further contribute in developing universal activities and strategies for oral health promotion so as ageing challenges are favoured.