bluuebunny/crossref_metadata_2025_split huggingface dataset:
Power Doppler Ultrasonography Assessment of Entheses in Spondyloarthropathies: Response to Therapy of Entheseal Abnormalities
Authors: NAREDO ESPERANZA , BATLLE-GUALDA ENRIQUE , GARCÍA-VIVAR M. LUZ , GARCÍA-APARICIO ANGEL M. , FERNÁNDEZ-SUEIRO JOSE LUIS , FERNÁNDEZ-PRADA MANUEL , GINER EMILIO , RODRIGUEZ-GOMEZ MANUEL , PINA MARIA FRANCISCA , MEDINA-LUEZAS JULIO A. , TOYOS FRANCISCO JAVIER , CAMPOS CRISTINA , GUTIÉRREZ-POLO RICARDO , FERRER MIGUEL ANGEL , MARTÍNEZ OLGA , DÍAZ-TORNE CESAR , GONZALEZ TERESA , CAMPOS SERAFÍN , QUEIRO RUBÉN , CASTAÑO-SÁNCHEZ MANUEL , AZNAR JUAN JOSÉ , BUSTABAD SAGRARIO , PAEZ-CAMINO MANUEL , TUNEU ROSER , RUIZ TERESA , MATEO LOURDES , PUJOL MANUEL , PONCE ANDRÉS , ROS INMACULADA , GALLEGOS ANGEL , MORENO JUAN , GUMBAU DOMINGO , SIANES MANUELA , POVEDA-ELICES M. JOSE , ROMERO-GÓMEZ MONTSERRAT , RAYA ENRIQUE 
Published: September 2010

Objective.To investigate the response to therapy of entheseal abnormalities assessed with power Doppler (PD) ultrasound (US) in spondyloarthropathies (SpA).Methods.A total of 327 patients with active SpA who were starting anti-tumor necrosis factor (TNF) therapy were prospectively recruited at 35 Spanish centers. A PDUS examination of 14 peripheral entheses was performed by the same investigator in each center at baseline and at 6 months. The following elementary lesions were assessed at each enthesis (presence/absence): morphologic abnormalities (hypoechogenicity and/or thickening), entheseal calcific deposits, cortical abnormalities (bone erosion and/or proliferation), adjacent bursitis and intraenthesis and perienthesis (tendon body and/or bursa) PD signal. Response to therapy of each elementary lesion was assessed by calculating change in the cumulative presence from baseline to 6 months. Intraobserver reliability of PDUS was evaluated by blindly assessing the stored baseline images 3 months after the real-time examination.Results.Complete data were obtained on 197 patients who received anti-TNF therapy for 6 months. In 91.4% of the patients there were gray-scale or PD elementary lesions at baseline and at 6 months. Cumulative entheseal morphologic abnormalities, intraenthesis PD, perienthesis PD, and bursitis showed a significant decrease from baseline to 6 months (p < 0.05). There was high intraobserver reliability for all elementary lesions (interclass correlation coefficient > 0.90, p < 0.0005).Conclusion.Entheseal morphologic abnormalities, PD signal, and bursitis were US abnormalities that were responsive to anti-TNF therapy in SpA. PDUS can be a reproducible method for multicenter monitoring of therapeutic response in enthesitis of SpA.
Predicting the Longer-term Outcomes of Total Hip Replacement
Authors: GANDHI RAJIV , DHOTAR HERMAN , DAVEY J. RODERICK , MAHOMED NIZAR N. 
Published: September 2010

Objective.The objective of this study was to identify the patient-level predictors (age, sex, body mass index, mental health, and comorbidity) for a sustained functional outcome at a minimum 1 year of followup after total hip replacement (THR).Methods.We reviewed data from our registry on 636 consecutive patients from 1998 to 2005. Demographic data and the outcome scores of the Western Ontario McMaster University Osteoarthritis Index (WOMAC) and Medical Outcomes Study Short-form 36 (SF-36) scores were extracted from the database. Longitudinal regression modeling was performed to identify the predictive factors of interest. Fourteen percent of patients were missing outcomes data at 1 year of followup.Results.The mean followup in our cohort was 3.3 years (range 1–6 yrs) and there were no revisions for aseptic loosening performed during this time. Mean clinical outcome scores were found to be relatively constant for the 6 years after surgery. Older age, year of followup, and greater comorbidity were identified as negative prognostic factors for a sustained functional outcome following THR (p < 0.05).Conclusion.Understanding of longterm surgical outcomes should be appropriately used to set realistic patient expectations of surgery.
Elevated Serum Glucose-6-Phosphate Isomerase Correlates with Histological Disease Activity and Clinical Improvement After Initiation of Therapy in Patients with Rheumatoid Arthritis
Authors: DAI LIE , ZHU LANG-JING , ZHENG DONG-HUI , MO YING-QIAN , WEI XIU-NING , SU JIAN-HUA , PESSLER FRANK , ZHANG BAI-YU 
Published: September 2010

Objective.To determine serum glucose-6-phosphate isomerase (GPI) concentrations in patients with rheumatoid arthritis (RA), and to test whether they correlate with objective measures of disease activity.Methods.Sera from 116 patients with RA, 69 patients with non-RA rheumatic diseases, and 101 healthy controls were analyzed. Levels of soluble serum GPI were measured by ELISA. Histological disease activity was determined with the synovitis score in synovial needle biopsies from 58 of the 116 patients with RA. Thirty-one of the 58 synovium samples were stained for CD68, CD3, CD20, CD38, CD79a, and CD34 by immunohistochemistry. Demographic data were collected, as well as serological and clinical variables that indicate RA disease activity, for Spearman correlation analysis.Results.Serum GPI level correlated positively with the synovitis score (r = 0.278, p = 0.034). Significantly higher soluble GPI levels were detected in the RA sera compared with sera from healthy controls and the non-RA disease controls (2.25 ± 2.82 vs 0.03 ± 0.05 and 0.19 ± 0.57 μg/ml, respectively; p < 0.0001). The rate of serum GPI positivity was significantly higher in the RA patients than in the non-RA disease controls (64.7% vs 10.1%; p < 0.0001). Spearman analysis showed no significant correlation between serum GPI level and Disease Activity Score in 28 joints at baseline. After initiation of antirheumatic treatments, GPI levels decreased significantly (2.81 ± 3.12 vs 1.44 ± 2.09 μg/ml; p = 0.016), paralleling improvement of the disease activity indices.Conclusion.Elevated serum GPI may be involved in the synovitis of RA and may prove useful as a serum marker for disease activity of RA.
pANCA, ASCA, and OmpC Antibodies in Patients with Ankylosing Spondylitis without Inflammatory Bowel Disease
Authors: de VRIES MIRJAM , van der HORST-BRUINSMA IRENE , van HOOGSTRATEN INGRID , van BODEGRAVEN ADRIAAN , von BLOMBERG MARY , RATNAWATI HANA , DIJKMANS BEN 
Published: September 2010

Objective.Patients with ankylosing spondylitis (AS) can suffer concurrently from inflammatory bowel disease (IBD), as ulcerative colitis (UC) or Crohn’s disease (CD). Serological markers have been described to diagnose IBD. We investigated IBD serological markers in AS patients without IBD and whether these antibodies enable differentiating patients with AS and IBD from those without IBD.Methods.Frequencies of perinuclear antineutrophil cytoplasmic antibodies (pANCA), antibodies to the cell-wall mannan of Saccharomyces cerevisiae (ASCA), and antibodies to porin protein C of Escherichia coli (OmpC) were evaluated in 179 patients: 52 with AS, 50 with UC, 51 with CD, and 26 with IBD and AS. Patient groups were matched for age and sex. All AS patients fulfilled the 1984 modified New York criteria. IBD was ascertained by clinical, endoscopic, and microscopic findings.Results.In 55% of the AS patients without manifest IBD at least one antibody associated with IBD was observed. pANCA, ASCA (IgA and/or IgG), and OmpC antibodies were found in 21%, 30%, and 19% of the AS patients, respectively. pANCA was more frequently present in AS with concurrent UC than in AS alone (OR 8.2, 95% CI 1.2–55.6), thus being an indicator for UC in AS patients.Conclusion.Antibodies associated with IBD are detectable in more than half of AS patients without symptoms or signs of IBD. A relatively recent marker in this setting, OmpC antibodies, does not contribute to the differentiation between AS and type of IBD. Presence of pANCA, however, is significantly increased in AS patients who also have UC, and is an indicator to perform endoscopy. These results corroborate a pathophysiological link between AS and IBD.
Impaired Gastric Emptying in Primary Sjögren’s Syndrome
Authors: HAMMAR OSKAR , OHLSSON BODIL , WOLLMER PER , MANDL THOMAS 
Published: September 2010

Objective.To investigate the prevalence of impaired gastric emptying (IGE) and its relation to autonomic nervous dysfunction (AD), functional bowel syndrome, and inflammatory and serological variables in patients with primary Sjögren’s syndrome (pSS).Methods.Twenty-eight patients with pSS according to the American-European Consensus Criteria were included in the study. Gastric emptying was evaluated by the octanoate breath test from which half-time (thalf) and lag-time (tlag) were determined and compared with the results from 50 healthy controls. Autonomic nervous function was evaluated by 5 objective autonomic reflex tests (ART) and by the Autonomic Symptom Profile (ASP) questionnaire evaluating AD symptoms. These results were compared with previously investigated healthy ART controls and population-based ASP controls. Patients were also assessed regarding symptoms of functional bowel syndrome.Results.The thalf and the tlag were significantly prolonged in patients compared to controls. Forty-three percent of patients with pSS presented signs of IGE and 29% fulfilled the criteria for gastroparesis. Significant correlations were found between tlag and increased levels of IgG (p = 0.02) and erythrocyte sedimentation rate (ESR; p = 0.01). In addition, rheumatoid factor (RF) seropositives showed objective signs of IGE to a greater extent than RF seronegatives. No associations between IGE, ART variables, ASP variables, or gastrointestinal symptoms were found.Conclusion.IGE was common in pSS. Associations with inflammatory and serological features of pSS could imply immunological mechanisms behind the IGE. Objective signs of IGE were not associated with objective signs or subjective symptoms of AD or functional bowel syndrome.
B Cell Subsets Contribute to Renal Injury and Renal Protection after Ischemia/Reperfusion
Authors: Renner Brandon , Strassheim Derek , Amura Claudia R. , Kulik Liudmila , Ljubanovic Danica , Glogowska Magdalena J. , Takahashi Kazue , Carroll Michael C. , Holers V. Michael , Thurman Joshua M. 
Published: September 2010

Abstract
Ischemia/reperfusion (I/R) triggers a robust inflammatory response within the kidney. Numerous components of the immune system contribute to the resultant renal injury, including the complement system. We sought to identify whether natural Abs bind to the postischemic kidney and contribute to complement activation after I/R. We depleted peritoneal B cells in mice by hypotonic shock. Depletion of the peritoneal B cells prevented the deposition of IgM within the glomeruli after renal I/R and attenuated renal injury after I/R. We found that glomerular IgM activates the classical pathway of complement, but it does not cause substantial deposition of C3 within the kidney. Furthermore, mice deficient in classical pathway proteins were not protected from injury, indicating that glomerular IgM does not cause injury through activation of the classical pathway. We also subjected mice deficient in all mature B cells (μMT mice) to renal I/R and found that they sustained worse renal injury than wild-type controls. Serum IL-10 levels were lower in the μMT mice. Taken together, these results indicate that natural Ab produced by peritoneal B cells binds within the glomerulus after renal I/R and contributes to functional renal injury. However, nonperitoneal B cells attenuate renal injury after I/R, possibly through the production of IL-10.
Inhibition of Hepatitis C Virus Replication by IFN-Mediated ISGylation of HCV-NS5A
Authors: Kim Min-Jung , Yoo Joo-Yeon 
Published: September 2010

Abstract
ISG15 is a ubiquitin-like molecule whose expression is induced by type I IFN (IFN-α/β) or in response to virus or bacterial infection. ISG15 or conjugation of ISG15 to target proteins was reported to play critical roles in the regulation of antiviral responses. IFN restricts replication of hepatitis C virus (HCV). However, molecular mechanism of IFN-α/β that inhibits HCV replication is not clear yet. In the current study, we demonstrated that replication of HCV was inhibited by overexpression of ISG15 and ISG15-conjugation enzymes in the HCV subgenomic replicon cells. Among various nonstructural proteins of HCV, NS5A was identified as the substrate for ISGylation. Furthermore, protein stability of NS5A was decreased by overexpression of ISG15 or ISG15-conjugating enzymes. The inhibitory effect of ISG15 or ISGylation on NS5A was efficiently blocked by substitution of lysine at 379 residue to arginine within the C-terminal region, suggesting that ISGylation directly controls protein stability of NS5A. Finally, the inhibitory effect of IFN-α/β on HCV replication was further enhanced by ISGylation, suggesting ISG15 as a therapeutic tool for combined therapy with IFN against HCV.
A Notch Ligand, Delta-Like 1 Functions As an Adhesion Molecule for Mast Cells
Authors: Murata Akihiko , Okuyama Kazuki , Sakano Seiji , Kajiki Masahiro , Hirata Tomohisa , Yagita Hideo , Zúñiga-Pflücker Juan Carlos , Miyake Kensuke , Akashi-Takamura Sachiko , Moriwaki Sawako , Niida Shumpei , Yoshino Miya , Hayashi Shin-Ichi 
Published: September 2010

Abstract
Mast cells (MCs) accumulate in chronic inflammatory sites; however, it is not clear which adhesion molecules are involved in this process. Recently, the expression of Notch ligands was reported to be upregulated in inflammatory sites. Although Notch receptors are known as signaling molecules that can activate integrins, their contributions to the adhesion of MCs have not been studied. In this study, we demonstrated that mouse MCs efficiently adhered to stromal cells forced to express a Notch ligand, Delta-like 1 (Dll1). Surprisingly, the adhesion was a consequence of direct cell–cell interaction between MCs and Dll1-expressing stromal cells rather than activation of downstream effectors of Notch receptor(s)-Dll1. The adhesion of MCs to Dll1-expressing stromal cells remained even when the cell metabolism was arrested. The recognition was blocked only by inhibition of Notch receptor(s)–Dll1 interaction by addition of soluble DLL1, or mAbs against Dll1 or Notch2. Taken together, these results indicate that Notch receptor(s) and Dll1 directly promote the adhesion of MCs to stromal cells by acting as adhesion molecules. This appreciation that Notch receptor–ligand interactions have an adhesion function will provide an important clue to molecular basis of accumulation of MCs to inflammatory sites.
Cutting Edge: MHC Class II Expression by Pulmonary Nonhematopoietic Cells Plays a Critical Role in Controlling Local Inflammatory Responses
Authors: Kreisel Daniel , Richardson Steven B. , Li Wenjun , Lin Xue , Kornfeld Christopher G. , Sugimoto Seiichiro , Hsieh Chyi-Song , Gelman Andrew E. , Krupnick Alexander S. 
Published: September 2010

Abstract
The interaction of CD4+ T cells with MHC class II (MHCII)-expressing hematopoietic APCs plays a critical role in both the generation of protective immune responses and maintenance of tolerance in the lung. The functional significance of MHCII expression by nonhematopoietic stromal cells, however, has not been defined in vivo. Using a novel mouse model of orthotopic left lung transplantation, we demonstrate that selective elimination of MHCII expression on nonhematopoietic cells leads to an inflammatory response as a result of reduced peripheral generation of regulatory CD4+ T cells. Absence of MHCII expression on nonhematopoietic cells also inhibits local growth of metastatic pulmonary tumor. These findings indicate that nonhematopoietic cells play a previously unrecognized role in downregulating inflammatory responses in nonlymphoid tissues.
Cutting Edge: Increased IL-17–Secreting T Cells in Children with New-Onset Type 1 Diabetes
Authors: Marwaha Ashish K. , Crome Sarah Q. , Panagiotopoulos Constadina , Berg Kyra B. , Qin Huilian , Ouyang Qin , Xu Lixin , Priatel John J. , Levings Megan K. , Tan Rusung 
Published: September 2010

Abstract
CD4+FOXP3+ regulatory T cells are essential for immune tolerance, and murine studies suggest that their dysfunction can lead to type 1 diabetes (T1D). Human studies assessing regulatory T cell dysfunction in T1D have relied on analysis of FOXP3-expressing cells. Recently, distinct subsets of CD4+FOXP3+ T cells with differing function were identified. Notably, CD45RA−CD25intFOXP3low T cells lack suppressive function and secrete the proinflammatory cytokine IL-17. Therefore, we evaluated whether the relative fractions of CD4+FOXP3+ subsets are altered in new-onset T1D subjects. We report that children with new-onset T1D have an increased proportion of CD45RA−CD25intFOXP3low cells that are not suppressive and secrete significantly more IL-17 than other FOXP3+ subsets. Moreover, these T1D subjects had a higher proportion of both CD4+ and CD8+ T cells that secrete IL-17. The bias toward IL-17–secreting T cells in T1D suggests a role for this proinflammatory cytokine in the pathogenesis of disease.
Generation, Isolation, and Maintenance of Human Mast Cells and Mast Cell Lines Derived from Peripheral Blood or Cord Blood
Authors: Rådinger Madeleine , Jensen Bettina M. , Kuehn Hye Sun , Kirshenbaum Arnold , Gilfillan Alasdair M. 
Published: September 2010

AbstractAntigen‐mediated mast cell activation is a pivotal step in the initiation of allergic disorders including anaphylaxis and atopy. To date, studies aimed at investigating the mechanisms regulating these responses, and studies designed to identify potential ways to prevent them, have primarily been conducted in rodent mast cells. However, to understand how these responses pertain to human disease, and to investigate and develop novel therapies for the treatment of human mast cell–driven disease, human mast cell models may have greater relevance. Recently, a number of systems have been developed to allow investigators to readily obtain sufficient quantities of human mast cells to conduct these studies. These mast cells release the appropriate suite of inflammatory mediators in response to known mast cell activators including antigen. These systems have also been employed to examine the signaling events regulating these responses. Proof of principle studies has also demonstrated utility of these systems for the identification of potential inhibitors of mast cell activation and growth. In this unit, techniques for the development and culture of human mast cells from their progenitors and the culture of human mast cell lines are described. The relative merits and drawbacks of each model are also described. Curr. Protoc. Immunol. 90:7.37.1‐7.37.12. © 2010 by John Wiley & Sons, Inc.
Effect of resveratrol on endothelial cell function: Molecular mechanisms
Authors: Schmitt Christoph A. , Heiss Elke H. , Dirsch Verena M. 
Published: September 2010

AbstractThe polyphenolic natural product resveratrol (RV), best known for its occurrence in grape skin and red wine, is considered a candidate drug for prevention and treatment of cardiovascular diseases. This review aims to summarize the molecular effects of RV on endothelial cells, which line the inner walls of blood vessels and play a key role in the development of those diseases. We describe how RV enhances endothelial nitric oxide production, improves endothelial redox balance and inhibits endothelial activation in response to pro‐inflammatory and metabolic insults. Furthermore, we summarize effects of RV on endothelial senescence, apoptosis, endothelin‐1 release, and endothelial progenitor cell function. As many of RV's actions seem to be mediated by SIRT1, different mechanistic possibilities how RV may lead to SIRT1 activation are discussed.
ChemInform Abstract: Endohedral and Exohedral Adsorption in C60: An Analytical Model
Authors: BRETON J. , GONZALEZ‐PLATAS J. , GIRARDET C. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The First Trigonal‐Bipyramidal Cluster with an Interstitial C2 Unit: ( Rb(Pr5(C2))Cl10).
Authors: MEYER G. , UHRLANDT S. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Electroanalytical Study of the Reduction of K2WCl6 in Molten LiCl‐KCl Eutectic.
Authors: SEQUEIRA C. A. C. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Binding of Fullerenes to Cadmium Sulfide and Cadmium Selenide Surfaces, Photoluminescence as a Probe of Strong, Lewis Acidity‐Driven, Surface Adduct Formation.
Authors: ZHANG J. Z. , GESELBRACHT M. J. , ELLIS A. B. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Studies on Crystalline Microporous Vanadium Silicates. Part 4. Synthesis, Characterization, and Catalytic Properties of V‐NCL‐1, a Large‐Pore Molecular Sieve.
Authors: REDDY K. R. , RAMASWAMY A. V. , RATNASAMY P. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Hydro(methano)thermal Synthesis and Characterization of Two Platinum Polysulfides: (Pt4S22)4‐ and (Pt(S4)2)2‐.
Authors: KIM K.‐W. , KANATZIDIS M. G. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Vibrational Spectra of 1,2,3‐Triazine
Authors: FISCHER G. , NWANKWOALA A. U. , OLLIFF M. P. , SCOTT A. P. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Luminescence and Charge Transfer. Part 3. The Use of Chromophores with ICT (Internal Charge Transfer) Excited States in the Construction of Fluorescent PET (Photoinduced Electron Transfer) pH Sensors and Related Absorption pH Sensors with Aminoalkyl Side Chains.
Authors: DE SILVA A. P. , GUNARATNE H. Q. N. , LYNCH P. L. M. , PATTY A. J. , SPENCE G. L. 
Published: September 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.