bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative analysis of the molecular starvation response of Southern Ocean copepods
Authors: Berger Cory A. , Steinberg Deborah K. , Copeman Louise A. , Tarrant Ann M. 
Published: May 2024

AbstractLarge lipid‐storing copepods dominate mesozooplankton biomass in the polar oceans and form a critical link between primary production and higher trophic levels. The ecological success of these species depends on their ability to survive periods of food deprivation in a highly seasonal environment, but the molecular changes that mediate starvation tolerance in these taxa are unknown. We conducted starvation experiments for two dominant Southern Ocean copepods, Calanoides acutus and Calanus propinquus, allowing us to compare the molecular starvation response between species. These species differ in life history, diet and metabolic traits, and expressed overlapping but distinct transcriptomic responses to starvation. Most starvation‐response genes were species‐specific, but we identified a conserved core set of starvation‐response genes related to RNA and protein metabolism. We used phylotranscriptomics to place these results in the context of copepod evolution and found that starvation‐response genes are under strong purifying selection at the sequence level and stabilizing selection at the expression level, consistent with their role in mediating essential biological functions. Selection on starvation‐response genes was especially strong in our focal lipid‐storing lineage relative to other copepod taxa, underscoring the significance of starvation tolerance for these species. We also found that certain key lipid enzymes (elongases and desaturases) have experienced diversification and positive selection in lipid‐storing lineages, reflecting the unique lipid storage needs of these animals. Our results shed light on the molecular adaptations of high‐latitude zooplankton to variable food conditions and suggest that starvation‐response genes are under particularly strong sequence and expression constraints.
Mitonuclear interactions impact aerobic metabolism in hybrids and may explain mitonuclear discordance in young, naturally hybridizing bird lineages
Authors: McDiarmid Callum S. , Hooper Daniel M. , Stier Antoine , Griffith Simon C. 
Published: May 2024

AbstractUnderstanding genetic incompatibilities and genetic introgression between incipient species are major goals in evolutionary biology. Mitochondrial genes evolve rapidly and exist in dense gene networks with coevolved nuclear genes, suggesting that mitochondrial respiration may be particularly susceptible to disruption in hybrid organisms. Mitonuclear interactions have been demonstrated to contribute to hybrid dysfunction between deeply divergent taxa crossed in the laboratory, but there are few empirical examples of mitonuclear interactions between younger lineages that naturally hybridize. Here, we use controlled hybrid crosses and high‐resolution respirometry to provide the first experimental evidence in a bird that inter‐lineage mitonuclear interactions impact mitochondrial aerobic metabolism. Specifically, respiration capacity of the two mitodiscordant backcrosses (with mismatched mitonuclear combinations) differs from one another, although they do not differ significantly from the parental groups or mitoconcordant backcrosses as we would expect of mitonuclear disruptions. In the wild hybrid zone between these subspecies, the mitochondrial cline centre is shifted west of the nuclear cline centre, which is consistent with the direction of our experimental results. Our results therefore demonstrate asymmetric mitonuclear interactions that impact the capacity of cellular mitochondrial respiration and may help to explain the geographic discordance between mitochondrial and nuclear genomes observed in the wild.
Do Turkey's 2024 Local Elections Signal the End of Erdoğan's Reign?
Authors: Yavuz M. Hakan , Koç Rasim 
Published: May 2024

AbstractThe unexpected victory of the opposition Republican People's Party in Turkey's March 31 municipal elections raises intriguing questions about the future of President Recep Tayyip Erdoğan after more than two decades of his rule. Just 10 months after Erdoğan won a new term, a significant portion of the electorate voted against him and his Justice and Development Party, marking his largest defeat ever. The president acknowledged the significance of the defeat, describing the results as “not only a loss of votes but also a loss of soul and blood.” This article analyzes the factors that drove the outcome and contends that this was more a protest of the Turkish strongman than an approval of opposition policies. The people voted against Erdoğan's arrogance and authoritarian style, as well as the poor quality of his party's candidates; the worsening economy, especially for retirees; and perceptions of a deteriorating justice system. The main opposition party also benefited from skillful leadership less than a year after its defeat in the presidential election. While the outcome of the 2024 vote signals a desire for democratic governance and accountability, it remains uncertain at this early stage whether this is a temporary deviation.
Influence of compression garments on proprioception: A systematic review and meta‐analysis
Authors: Ghai Shashank , Nilson Finn , Gustavsson Johanna , Ghai Ishan 
Published: May 2024

AbstractCompression garments (CGs) are commonly used in rehabilitation and sports contexts to enhance performance and speed up recovery. Despite the growing use of CGs in recent decades, there is no unanimous consensus on their overall influence on joint proprioception. In this current meta‐analysis, we aim to fill this knowledge gap by assessing the impact of CGs on joint proprioception. We conducted a literature search across seven databases and one registry. Ultimately, we included 27 studies with 671 participants. The meta‐analysis revealed that wearing CGs resulted in a significant reduction in absolute error during joint position sensing (Hedges’ g: −0.64, p = 0.006) as compared to no CGs. However, further analyses of variables such as constant error (p = 0.308), variable error (p = 0.541) during joint position sense tests, threshold to detect passive motion (p = 0.757), and active movement extent discrimination (p = 0.842) did not show a significant impact of CGs. The review also identified gaps in the reporting of certain outcomes, such as parameters of CGs, reporting of performance, individual‐reported outcomes, and lack of placebo comparators. Consequently, this review provides guidelines for future studies that may facilitate evidence‐based synthesis and ultimately contribute to a better understanding of the overall influence of CGs on joint proprioception.
Efficacy of antimalarials in oral lichen planus: A systematic review
Authors: Tillero Rosana , González‐Serrano José , Caponio Vito Carlo Alberto , Serrano Julia , Hernández Gonzalo , López‐Pintor Rosa María 
Published: May 2024

AbstractObjectiveTo evaluate whether hydroxychloroquine (HCQ) or chloroquine (CQ) are effective for the treatment of oral lichen planus (OLP).Materials and MethodsA literature search was conducted in four databases. Clinical studies investigating the effect of HCQ/CQ in patients with OLP were included.ResultsEleven studies were included. Four were RCTs and seven quasi‐experimental studies. The studies included 390 patients diagnosed with OLP, of which 326 and 7 received HCQ and CQ, respectively. 46 patients received topical dexamethasone, 5 placebo and 6 griseofulvin as controls. Five studies assessed pain, and all of them obtained pain reduction with the use of HCQ. Six studies reported objective clinical improvement of OLP with the use of HCQ. Five studies that used a subjective scale obtained that 24%–100% of the patients achieved a complete/almost complete improvement of OLP lesions and its symptomatology. The most frequent side effects were vision problems, gastric discomfort, rash, nauseas, headaches, skin pigmentation, and elevated kidney function. 17 patients had to withdraw from the studies.ConclusionsCurrent evidence is scarce to confirm HCQ as a therapeutic option for OLP. More RCTs are needed to compare its efficacy with topical corticosteroids and to evaluate whether HCQ reduces relapses of OLP.
Periodontitis and systemic parameters in chronic kidney disease: Systematic review and meta‐analysis
Authors: Ferreira Suélem Maria Santana Pinheiro , Gomes‐Filho Isaac Suzart , Costa Maria da Conceição Nascimento , Cruz Simone Seixas da , Neves Carolina Lara , Silva Carlos Alberto Lima da , Andrade Kaio Vinicius Freitas de , Moreira Marcela Beatriz Aguiar , Hintz Alexandre Marcelo , Vianna Maria Isabel Pereira 
Published: May 2024

AbstractObjectiveTo perform a systematic review with meta‐analysis to assess recent scientific evidence on the association between periodontitis and systemic parameters/conditions in individuals with chronic kidney disease (CKD).Materials and MethodsThe search for studies was performed in MedLine/PubMed, Scopus, Web of Science, and BIREME databases. Reference lists of selected articles were also searched. Studies with different epidemiological designs evaluating the influence of exposure to periodontitis on serum markers and mortality in individuals with CKD were eligible for inclusion. Three independent reviewers performed the article selection and data extraction. The assessment of methodological quality used the adapted Newcastle Ottawa Scale. Random effects meta‐analysis was performed to calculate association measurements and 95% confidence intervals.ResultsIn total, 3053 records were identified in the database search, with only 25 studies meeting the eligibility criteria and, of these, 10 studies contributed data for meta‐analysis. Using a random‐effects model, periodontitis was associated with hypoalbuminemia (PRunadjusted = 2.47; 95%CI:1.43–4.26), with high levels of C‐reactive protein (PRunadjusted = 1.35; 95%CI%:1.12–1.64), death from cardiovascular disease (RRunadjusted = 2.29; 95%CI:1.67–3.15) and death from all causes (RRunadjusted = 1.73; 95%CI:1.32–2.27).ConclusionsThe findings of this review validated a positive association between periodontitis and serum markers and mortality data in individuals with CKD.
Extended commentary—U.S.‐Asian policy in the throes of McCarthyism, old and new
Authors: Thornton William H. , Thornton Songok Han 
Published: May 2024

AbstractThe subtext of this extended commentary for the 2024 Policy Studies Yearbook in Politics & Policy is the crucial role that liberalism and civil rights must play in U.S. foreign policy if America is to be on the right side of history. Our explicit subject is the conflux of elite and populist forces that constantly work against such liberal‐democratic engagement. It is no secret that today's MAGAism (“Make America Great Again”) wears many of the same antiliberal stripes as did postwar McCarthyism, which was its direct progenitor. Thanks largely to the center‐right resistance of President Eisenhower, McCarthyism was ultimately held in check domestically. Unfortunately, that was not the case on the side of U.S. foreign policy, where tenacious strains of antiliberalism had a very long shelf life. Thus, McCarthyism ended up having its most durable impact in the developing world, where cozy relations with highly reactionary regimes were considered necessary in the face of communist inroads. At a time when liberalism was hitting its full stride on American soil, U.S. foreign policy was exporting a very different America abroad, and especially in Asia. Even domestically, the waspish virulence of McCarthyism was never eradicated. It simply hibernated, and in 2016, it came back with a vengeance in the even more truculent form of Trumpism. Once again, xenophobic populism is laying claim to U.S. foreign policy and savaging liberal internationalism. The entrenched progressivism that stood its ground against McCarthyism has long since eroded. MAGAism faces no obstacle comparable to the dynamic liberalism of the 1950s or the counterculture of the 1960s. Seen from the perspective of January 6, 2021, the old McCarthyism looks tame by comparison.Related ArticlesCraig, Stephen C., and Jason Gainous. 2024. “To Vote or Not to Vote? Fake News, Voter Fraud, and Support for Postponing the 2020 U.S. Presidential Election.” Politics & Policy 52(1): 33–50. https://doi.org/10.1111/polp.12577.Haar, Roberta N., and Lutz F. Krebs. 2021. “The Failure of Foreign Policy Entrepreneurs in the Trump Administration.” Politics & Policy 49(2): 446–78. https://doi.org/10.1111/polp.12399.Skidmore, Max J. 2023. “Abortion—Reactionary Theocracy Rises in America, While Declining Elsewhere.” Politics & Policy 51(3): 437–57. https://doi.org/10.1111/polp.12534.
The ripple effect: How trade policy shocks impact innovation of Chinese firms
Authors: Meng Ning , Ni Yining , Ma Yeqing 
Published: May 2024

AbstractThis article examines how trade policy shocks impact firm innovation indirectly through information sharing across firms. Using a unique dataset of Chinese firms between 2000 and 2013, we identify connections between firms by exploiting spillover effects of antidumping. Empirical results show that antidumping has negative spillover effects on firm innovation through information exchanges across firms in market or product networks. Trade restrictions on extensive and intensive margins work as important channels through which the networks impact firm innovation. Our findings provide new empirical evidence on indirect effects of antidumping on firm innovation and better understanding on channels of trade policy shocks.
M2WISH: An easy and efficient protocol for whole‐mount mRNAin situ hybridization that allows 3D cell resolution of gene expression in Arabidopsis thaliana
Authors: Chelysheva Liudmila , Morin Halima , Biot Eric , Nicolas Antoine , Rech Philippe , da Costa Marco , Barel Lisa , Laufs Patrick , Palauqui Jean‐Christophe 
Published: May 2024

SUMMARYGene expression analysis is essential for understanding the mechanisms involved in plant development. Here, we developed M2WISH, a protocol based on MicroWave treatment for Wholemount mRNA In Situ Hybridization in Arabidopsis. By permeabilizing tissues without damaging cellular organization this protocol results in high and homogeneous hybridization yields that enable systematic analysis of gene expression dynamics. Moreover, when combined with cellular histochemical staining, M2WISH successfully provides a cellular resolution of gene expression. Thus, we demonstrate the robustness of M2WISH with 10 genes on roots, aerial meristems, leaves, and embryos in the seed. We applied M2WISH to study the spatial dynamics of WUSCHEL (WUS) and CLAVATA3 (CLV3) expression during in vitro meristematic conversion of roots into shoot apical meristems. Thus, we showed that shoot apical meristems could arise from two different types of root structures that differed by their CLV3 gene expression patterns. We constructed 3D cellular representations of WUS and CLV3 gene co‐expression pattern and stressed the variability inherent to meristem conversion. Thus, this protocol generates a large amount of data on the localization of gene expression, which can be used to model complex systems.
Prognostic value of pulmonary diffusing capacity for carbon monoxide and ventilation–perfusion SPECT findings in pulmonary arterial hypertension
Authors: Mohammad Milan , Hartmann Jacob P. , Carlsen Jørn , Greve Anders M. , Berg Ronan M. G. , Mortensen Jann 
Published: May 2024

AbstractReduced pulmonary diffusing capacity for carbon monoxide (DLCO) can be observed in pulmonary arterial hypertension (PAH) and associates with increased mortality. However, the prognostic value of DLCO when corrected for haemoglobin (DLCOc), an independent modifier of DLCO, remains understudied. Additionally, the prognostic role of ventilation (V)–perfusion (Q) emission computed tomography (V/Q SPECT) findings in patients with PAH, which may concurrently be performed to rule out chronic thromboembolic pulmonary hypertension, is uncertain. A retrospective cohort study was conducted on 152 patients with PAH referred to a tertiary hospital for evaluation from January 2011 to January 2020. Lung function tests, clinical data and V/Q SPECT were ascertained. Cox regression analysis was performed to evaluate the association between DLCOc, DLCO and V/Q SPECT defects at referral with all‐cause mortality. In equally adjusted Cox regression analysis, each percentage increase in DLCOc % predicted (%pred) (hazard ratio (HR) 0.97; 95% CI: 0.94–0.99) and DLCO%pred (HR 0.97; 95% CI: 0.94–0.99) was similarly associated with all‐cause mortality. There was no detectable difference in area under the curve for prediction of all‐cause mortality by DLCOc%pred and DLCO%pred (C‐index 0.71 and 0.72, respectively, P = 0.85 for difference). None of the defects noted on V/Q SPECT were significantly associated with mortality, but mismatched defects were associated with lower values of DLCOc%pred and DLCO%pred. DLCOc%pred and DLCO%pred perform equally as prognostic markers in PAH, supporting the use of either metric when available for prognostic stratification.
Advancements and Challenges in Integrating Renewable Energy Sources Into Distribution Grid Systems: A Comprehensive Review
Authors: Singh Surender , Singh Saurabh 
Published: May 2024

Abstract
The issues in integrating renewable energy sources (RES) into distribution grid structures are thoroughly examined in this research. It highlights how important this integration is to updating the energy system and attaining environmental goals. The study explores the specific problems confronted by means of on-grid power structures, along with overall performance metrics and compatibility issues. Additionally, it presents a thorough assessment of the attributes of various RES hybrid systems, together with technology from the fields of solar, wind, batteries, and biomass. To be able to spotlight the significance of innovative solutions inside the dispersed technology environment, the integration of RES with combined heat and power system structures is investigated. This study addresses the numerous problems with RES integration into the grid to better comprehend their intricacies. The viability of RES integration is supported by real-world case studies that provide operational examples of dispersed generation systems. The study concludes by discussing the technical, financial, and grid-related problems associated with distributed generating systems' limits and highlighting the contribution of cutting-edge technology and artificial intelligence to their removal. In conclusion, the report highlights the development toward smarter grids and improved distributed generating capacities as the essential component of a robust and sustainable energy future.
Accumulation of resistance genes in
Salmonella
Typhimurium transmitted between poultry and dairy farms increases the risk to public health
Authors: Perry Janet , Arnold Katya , Satuchne Cinthia , Koren Ori , Kenigswald Gaby , Elnekave Ehud 
Published: May 2024

ABSTRACT

Salmonella
Typhimurium is a zoonotic pathogen that poses a major threat to public health. This generalist serotype can be found in many hosts and the environment where varying selection pressures may result in the accumulation of antimicrobial resistance determinants. However, the transmission of this serotype between food-producing hosts, specifically between poultry layer flocks and nearby dairy herds, was never demonstrated. We investigated an outbreak at a dairy in Israel to determine the role of nearby poultry houses to be sources of infection. The 2-month outbreak resulted in a 47% mortality rate among 15 calves born in that period. Routine treatment of fluid therapy, a nonsteroidal anti-inflammatory, and cefquinome was ineffective, and control was achieved by the introduction of vaccination of dry cows against
Salmonella
(Bovivac S, MSD Animal Health) and a strict colostrum regime. Whole genome sequencing and antimicrobial sensitivity tests were performed on
S
. Typhimurium strains isolated from the dairy (
n
= 4) and strains recovered from poultry layer farms (
n
= 10). We identified acquired antimicrobial-resistant genes, including the
bla
CTX-M-55
gene, conferring resistance to extended-spectrum cephalosporins, which was exclusive to dairy isolates. Genetic similarity with less than five single nucleotide polymorphism differences between dairy and poultry strains suggested a transmission link. This investigation highlights the severe impact of
S
. Typhimurium on dairy farms and the transmission risk from nearby poultry farms. The accumulation of potentially transferable genes conferring resistance to critically important antimicrobials underscores the increased public health risk associated with
S
. Typhimurium circulation between animal hosts.

IMPORTANCE

Salmonella
Typhimurium is one of the major causes of food-borne illness globally. Infections may result in severe invasive disease, in which antimicrobial treatment is warranted. Therefore, the emergence of multi-drug-resistant strains poses a significant challenge to successful treatment and is considered one of the major threats to global health.
S
. Typhimurium can be found in a variety of animal hosts and environments; however, its transmission between food-producing animals, specifically poultry layers flocks and dairy herds, was never studied. Here, we demonstrate the transmission of the pathogen from poultry to a nearby dairy farm. Alarmingly, the multi-drug-resistant strains collected during the outbreak in the dairy had acquired resistance to extended-spectrum cephalosporins, antibiotics critically important in treating Salmonellosis in humans. The findings of the study emphasize the increased risk to public health posed by zoonotic pathogens’ circulation between animal hosts.
CRISPR/Cas9 gene targeting plus nanopore DNA sequencing with the plasmid pBR322 in the classroom
Authors: Wünschiers Röbbe , Leidenfrost Robert Maximilian , Holtorf Hauke , Dittrich Bernd , Dürr Thomas , Braun Jürgen 
Published: May 2024

ABSTRACT

Both nanopore-based DNA sequencing and CRISPR/Cas-based gene editing represent groundbreaking innovations in molecular biology and genomics, offering unprecedented insights into and tools for working with genetic information. For students, reading, editing, and even writing DNA will be part of their everyday life. We have developed a laboratory procedure that includes (i) the biosynthesis of a guide RNA for, (ii) targeting Cas9 to specifically linearize the pBR322 plasmid, and (iii) the identification of the cutting site through nanopore DNA sequencing. The protocol is intentionally kept simple and requires neither living organisms nor biosafety laboratories. We divided the experimental procedures into separate activities to facilitate customization. Assuming access to a well-equipped molecular biology laboratory, an initial investment of approximately $2,700 is necessary. The material costs for each experiment group amount to around $130. Furthermore, we have developed a freely accessible website (
https://dnalesen.hs-mittweida.de
) for sequence read analysis and visualization, lowering the required computational skills to a minimum. For those with strong computational skills, we provide instructions for terminal-based data processing. With the presented activities, we aim to provide a hands-on experiment that engages students in modern molecular genetics and motivates them to discuss potential implications. The complete experiment can be accomplished within half a day and has been successfully implemented by us at high schools, in teacher training, and at universities. Our tip is to combine CRISPR/Cas gene targeting with nanopore-based DNA sequencing. As a tool, we provide a
website
that facilitates sequence data analysis and visualization.