bluuebunny/crossref_metadata_2025_split huggingface dataset:
Causal role of immune cells in Hashimoto’s thyroiditis: Mendelian randomization study
Authors: Zhao Zhendan , Gao Yuehua , Pei Xiaoqing , Wang Wenhao , Zhang Huawei 
Published: May 2024

ObjectivesHashimoto’s thyroiditis (HT) is a common autoimmune disease whose etiology involves a complex interplay between genetics and environment. Previous studies have demonstrated an association between immune cells and HT. However, the casual relationship was not clear. We aimed to explore the causal associations between signatures of immune cells and HT.MethodsIn this study, bidirectional two-sample Mendelian randomization (MR) analysis was conducted to investigate the potential causal relationship between 731 immune cell signatures and HT by using genome-wide association study (GWAS) data. Heterogeneity and horizontal pleiotropy were detected through extensive sensitivity analyses.ResultsThe increased levels of six immune phenotypes were observed to be causally associated with increased risk of HT P &lt; 0.01, which were CD3 on CM CD8br, CD3 on CD39+ secreting Treg, HLA DR on CD33dim HLA DR+ CD11b−, CD3 on CD4 Treg, CD62L− plasmacytoid DC %DC, and CD3 on CD45RA+ CD4+. In addition, the levels of FSC-A on HLA DR+ T cell and CD62L on monocyte were associated with disease risk of HT P &lt; 0.01. In addition, HT also had causal effects on CD3 on CM CD8br, CCR2 on monocyte, CD25 on CD39+ resting Treg, and CCR2 on CD62L+ myeloid DC P &lt; 0.05.ConclusionsIn this study, we demonstrated the genetic connection between immune cell traits and HT, thereby providing guidance and direction for future treatment and clinical research.
CCL18 aggravates atherosclerosis by inducing CCR6-dependent T-cell influx and polarization
Authors: Singh Anjana , Kraaijeveld Adriaan O. , Curaj Adelina , Wichapong Kanin , Hammerich Linda , de Jager Saskia C. A. , Bot Ilze , Atamas Sergei P. , van Berkel Theo J. C. , Jukema J. Wouter , Comerford Iain , McColl Shaun R. , Mees Barend , Heemskerk Johan W. M. , Nicolaes Gerry A. F. , Hackeng Tilman , Liehn Elisa Anamaria , Tacke Frank , Biessen Erik A. L. 
Published: May 2024

IntroductionThe CC chemokine ligand 18 (CCL18) is a chemokine highly expressed in chronic inflammation in humans. Recent observations of elevated CCL18 plasma levels in patients with acute cardiovascular syndromes prompted an investigation into the role of CCL18 in the pathogenesis of human and mouse atherosclerosis.Methods and resultsCCL18 was profoundly upregulated in ruptured human atherosclerotic plaque, particularly within macrophages. Repeated administration of CCL18 in Western-type diet–fed ApoE−/− mice or PCSK9mut-overexpressing wild type (WT) mice led to increased plaque burden, enriched in CD3+ T cells. In subsequent experimental and molecular modeling studies, we identified CCR6 as a functional receptor mediating CCL18 chemotaxis, intracellular Ca2+ flux, and downstream signaling in human Jurkat and mouse T cells. CCL18 failed to induce these effects in vitro in murine spleen T cells with CCR6 deficiency. The ability of CCR6 to act as CCL18 receptor was confirmed in vivo in an inflammation model, where subcutaneous CCL18 injection induced profound focal skin inflammation in WT but not in CCR6−/− mice. This inflammation featured edema and marked infiltration of various leukocyte subsets, including T cells with a Th17 signature, supporting CCR6’s role as a Th17 chemotactic receptor. Notably, focal overexpression of CCL18 in plaques was associated with an increased presence of CCR6+ (T) cells.DiscussionOur studies are the first to identify the CCL18/CCR6 axis as a regulator of immune responses in advanced murine and human atherosclerosis.
Microplastics dysregulate innate immunity in the SARS-CoV-2 infected lung
Authors: Bishop Cameron R. , Yan Kexin , Nguyen Wilson , Rawle Daniel J. , Tang Bing , Larcher Thibaut , Suhrbier Andreas 
Published: May 2024

IntroductionGlobal microplastic (MP) pollution is now well recognized, with humans and animals consuming and inhaling MPs on a daily basis, with a growing body of concern surrounding the potential impacts on human health.MethodsUsing a mouse model of mild COVID-19, we describe herein the effects of azide-free 1 μm polystyrene MP beads, co-delivered into lungs with a SARS-CoV-2 omicron BA.5 inoculum. The effect of MPs on the host response to SARS-CoV-2 infection was analysed using histopathology and RNA-Seq at 2 and 6 days post-infection (dpi).ResultsAlthough infection reduced clearance of MPs from the lung, virus titres and viral RNA levels were not significantly affected by MPs, and overt MP-associated clinical or histopathological changes were not observed. However, RNA-Seq of infected lungs revealed that MP exposure suppressed innate immune responses at 2 dpi and increased pro-inflammatory signatures at 6 dpi. The cytokine profile at 6 dpi showed a significant correlation with the ‘cytokine release syndrome’ signature observed in some COVID-19 patients.DiscussionThe findings are consistent with the recent finding that MPs can inhibit phagocytosis of apoptotic cells via binding of Tim4. They also add to a growing body of literature suggesting that MPs can dysregulate inflammatory processes in specific disease settings.
Global research progress of gut microbiota and epigenetics: bibliometrics and visualized analysis
Authors: Tian Siyu , Chen Min 
Published: May 2024

BackgroundGut microbiota is an important factor affecting host health. With the further study of the mechanism of gut microbiota, significant progress has been made in the study of the link between gut microbiota and epigenetics. This study visualizes the body of knowledge and research priorities between the gut microbiota and epigenetics through bibliometrics.MethodsPublications related to gut microbiota and epigenetics were searched in the Web of Science Core Collection (WoSCC) database. Vosviewer 1.6.17 and CiteSpace 6.1.R2 were used for bibliometric analysis.ResultsWoSCC includes 460 articles from 71 countries. The number of publications on gut microbiota and epigenetics has increased each year since 2011. The USA, PEOPLES R CHINA, and ITALY are at the center of this field of research. The University of California System, Harvard University, and the University of London are the main research institutions. Li, X, Yu, Q, Zhang, S X are the top authors in this research field. We found that current research hotspots and frontiers include short-chain fatty acids (SCFA) play an important role in gut microbiota and epigenetic mechanisms, gut microbiota and epigenetics play an important role in host obesity, diet, and metabolism. Gut microbiota and epigenetics are closely related to colorectal cancer, breast cancer, and inflammatory bowel disease. At the same time, we found that gut microbiota regulates epigenetics through the gut-brain axis and has an impact on psychiatric diseases. Therefore, probiotics can regulate gut microbiota, improve lifestyle, and reduce the occurrence and development of diseases.ConclusionThis is the first comprehensive and in-depth bibliometric study of trends and developments in the field of gut microbiota and epigenetics research. This study helps to guide the direction of research scholars in their current field of study.
Case report: Endobronchial closure of postoperative bronchopleural fistula with embolization coil: a sandwich-like approach
Authors: Bai Yang , Chi Jing , Wang Hansheng , Li Yishi , Guo Shuliang 
Published: May 2024

BackgroundEmbolization Coil has been reported to effectively treat postoperative bronchopleural fistula (BPF). Little detailed information was available on computer tomography (CT) imaging features of postoperative BPF and treating procedures with pushable Embolization Coil.ObjectiveWe aimed to specify the imaging characteristics of postoperative BPFs and present our experience treating them with the pushable Embolization Coil.MethodsSix consecutive patients (four males and two females aged 29–56 years) diagnosed with postoperative BPF receiving bronchoscopic treatment with the pushable Nester® Embolization Coil (Cook Medical, Bloomington, Indiana) were included in this single-center, retrospective study. Multiplanar reconstruction of multidetector CT scans was reviewed for the presence, location, size, and radiological complications of each BPF, including air collection, pneumothorax, bronchiectasis, and chest tube. Using standardized data abstraction forms, demographic traits and clinical outcomes were extracted from the medical files of these patients.ResultsThe underlying diseases for lung resection surgery were pulmonary tuberculosis (n = 3), lung adenocarcinoma (n = 2), and pulmonary aspergillosis (n = 1). All patients had air or air-fluid collection with chest tubes on radiological findings. Multiplanar reconstruction identified the presence of postoperative BPF in all patients. Five fistulas were central, located proximal to the main or lobar bronchus, while one was peripheral, distant from the lobar bronchus. Fistula sizes ranged from 0.8 to 5.8 mm. Subsequent bronchoscopy and occlusion testing confirmed fistula openings in the bronchial stump: right main bronchus (n = 1), right upper lobe (n = 2), and left upper lobe (n = 3). The angioplasty catheter-based procedure allows precise fistula occlusion “like a sandwich” with the pushable Embolization Coil. Five patients with BPF sizes ranging from 0.8 to 1.5 mm were successfully treated with a pushable Embolization Coil, except for one with a BPF size of 5.8 mm. No adverse events or complications were observed throughout follow-up, ranging from 29 to 1,307 days.ConclusionThe pushable Nester® Embolization Coil seems a minimally invasive, cost-effective, and relatively easy-to-perform bronchoscopic treatment for postoperative BPF with a size less than 2 mm. Further studies are required to ensure the use of pushable Embolization Coil in treating postoperative BPF.
Chloroquine-induced DNA damage synergizes with DNA repair inhibitors causing cancer cell death
Authors: Iglesias-Corral Diego , García-Valles Paula , Arroyo-Garrapucho Nuria , Bueno-Martínez Elena , Ruiz-Robles Juan Manuel , Ovejero-Sánchez María , González-Sarmiento Rogelio , Herrero Ana Belén 
Published: May 2024

BackgroundCancer is a global health problem accounting for nearly one in six deaths worldwide. Conventional treatments together with new therapies have increased survival to this devastating disease. However, the persistent challenges of treatment resistance and the limited therapeutic arsenal available for specific cancer types still make research in new therapeutic strategies an urgent need.MethodsChloroquine was tested in combination with different drugs (Panobinostat, KU-57788 and NU-7026) in 8 human-derived cancer cells lines (colorectal: HCT116 and HT29; breast: MDA-MB-231 and HCC1937; glioblastoma: A-172 and LN-18; head and neck: CAL-33 and 32816). Drug´s effect on proliferation was tested by MTT assays and cell death was assessed by Anexin V-PI apoptosis assays. The presence of DNA double-strand breaks was analyzed by phospho-H2AX fluorescent staining. To measure homologous recombination efficiency the HR-GFP reporter was used, which allows flow cytometry-based detection of HR stimulated by I-SceI endonuclease-induced DSBs.ResultsThe combination of chloroquine with any of the drugs employed displayed potent synergistic effects on apoptosis induction, with particularly pronounced efficacy observed in glioblastoma and head and neck cancer cell lines. We found that chloroquine produced DNA double strand breaks that depended on reactive oxygen species formation, whereas Panobinostat inhibited DNA double-strand breaks repair by homologous recombination. Cell death caused by chloroquine/Panobinostat combination were significantly reduced by N-Acetylcysteine, a reactive oxygen species scavenger, underscoring the pivotal role of DSB generation in CQ/LBH-induced lethality. Based on these data, we also explored the combination of CQ with KU-57788 and NU-7026, two inhibitors of the other main DSB repair pathway, nonhomologous end joining (NHEJ), and again synergistic effects on apoptosis induction were observed.ConclusionOur data provide a rationale for the clinical investigation of CQ in combination with DSB inhibitors for the treatment of different solid tumors.
Enhancing Cancer Therapy: Boron-Rich Polyboronate Ester Micelles for Synergistic Boron Neutron Capture Therapy and PD-1/PD-L1 Checkpoint Blockade
Authors: Chiu Yi-Lin , Fu Wan Yun , Huang Wei-Yuan , Hsu Fang-Tzu , Chen Hsin-Wei , Wang Tzu-Wei , Keng Pei Yuin 
Published: May 2024

Malignant cancers, known for their pronounced heterogeneity, pose substantial challenges to monotherapeutic strategies and contribute to the risk of metastasis. Addressing this, our study explores the synergistic potential of combining boron neutron capture therapy (BNCT) with immune checkpoint blockade to enhance cancer treatment efficacy. We synthesized boron-rich block copolymer micelles as a novel boron drug for BNCT. Characterization was conducted using nuclear magnetic resonance, gel-permeation chromatography, transmission electron microscopy, and dynamic light scattering. These micelles, with an optimal size of 91.3 nm and a polydispersity index of 0.18, are suitable for drug delivery applications. In vitro assessments on B16-F10 melanoma cells showed a 13-fold increase in boron uptake with the micelles compared to borophenyl alanine (BPA), the conventional boron drug for BNCT. This resulted in a substantial increase in BNCT efficacy, reducing cell viability to 77% post-irradiation in micelle-treated cells, in contrast to 90% in BPA-treated cells. In vivo, melanoma-bearing mice treated with these micelles exhibited an 8-fold increase in boron accumulation in tumor tissues versus those treated with BPA, leading to prolonged tumor growth delay (5.4 days with micelles versus 3.3 days with BPA). Moreover, combining BNCT with anti-PD-L1 immunotherapy further extended the tumor growth delay to 6.6 days, and enhanced T-cell infiltration and activation at tumor sites, thereby indicating a boosted immune response. This combination demonstrates a promising approach by enhancing cytotoxic T-cell priming and mitigating the immunosuppressive effects of melanoma tumors.
High-Frequency Optical Coherence Elastography for Gingival Tissue Characterization: Variability in Stiffness and Response to Physiological Conditions
Authors: Moon Wonjoon , Feng Xu , Li Guo-Yang , Yun Seok-Hyun 
Published: May 2024

Accurate measurement of gingiva’s biomechanical properties in vivo has been an active field of research but remained an unmet challenge. Currently, there are no noninvasive tools that can accurately quantify tensile and shear moduli, which govern gingival health, with sufficiently high accuracy. This study presents the application of high-frequency optical coherence elastography (OCE) for characterizing gingival tissue in both porcine models and human subjects. Dynamic mechanical analysis, histology studies, and strain analysis are performed to support the OCE result. Our findings demonstrate substantial differences in tissue stiffness between supra-dental and inter-dental gingiva, validated by dynamic mechanical analysis and OCE. We confirmed the viscoelastic, nearly linear, and transverse-isotropic properties of gingiva in situ, establishing the reliability of OCE measurements. Further, we investigated the effects of tissue hydration, collagen degradation, and dehydration on gingival stiffness. These conditions showed a decrease and increase in stiffness, respectively. While preliminary, our study suggests OCE’s potential in periodontal diagnosis and oral tissue engineering, offering real-time, millimeter-scale resolution assessments of tissue stiffness, crucial for clinical applications and biomaterial optimization in reconstructive surgeries.
HUBUNGAN KENAIKAN BERAT BADAN IBU HAMIL TERHADAP BERAT BADAN LAHIR BAYI DI PMB SAGITA
Authors: Apriyani Titin , Rahma Melia , Anggraini Ririn 
Published: May 2024

Gizi ibu hamil merupakan salah satu fokus perhatian kegiatan perbaikan gizi masyarakat karena dampaknya yang signifikan terhadap kondisi janin yang dikandungnya, peningkatan berat badan dan nutrisi ibu selama kehamilan sangat penting untuk dipantau, karena merupakan indikator pertumbuhan dan perkembangan janin. Tujuan penelitianadalah mengetahui hubungan kenaikan berat badan ibu hamil terhadap berat badan lahir bayi di PMB Sagita Palembang. Metode penelitian menggunakan deskriptif analitik dengan pendekatan cross sectional. Populasi dalam penelitian ini adalah seluruh ibu yang melahirkan bayinya di PMB Sagita Palembang. Pengambilan sampel menggunakan teknik purposive sampling. Penelitian ini dilakukan di PMB Sagita Palembang pada bulan Mei 2022. Data yang digunakan adalah data sekunder, data diambil dari catatan bidan dengan melihat kenaikan berat badan ibu pada masa hamil dan berat badan bayi ketika lahir, data dianalisis secara univariat dan bivariat dengan menggunakan uji statistic chi-square. Hasil penelitian menunjukkan bahwa dari 53 responden, ibu hamil dengan kategori normal sebanyak 45,3% dan pada kategori tidak normal sebanyak 61,5%, berat badan lahir bayi pada rentang 2500-4000 gram sebanyak 92,4%, BB <2500 gram sebanyak 3,8% dan BB >4000 gram sebanyak 3,8%. Hasil uji chi squaredidapatkan nilai ρ-value 0,279 > ɑ (0,05). Kesimpulan: Tidak terdapat hubungan yang bermakna antara kenaikan BB ibu hamil terhadap berat badan lahir bayi di PMB Sagita.
PREVALÊNCIA DE ANOSMIA E DISGEUSIA EM PACIENTES VÍTIMAS DA SARS- CoV – 2
Authors: Nascimento Camila Silva Do , Lima Iasmyn Bastos , Mendonça Suellen da Silva , Maciel Thaise Maria Oliveira , Ribas Izis Gabrielle Cordeiro , Sousa Vitoria Lorena Tavares , Costa Jayanne Marques Bitencourt da , Vidal Ana Lucia Farias , Melo Renata Amanajás de , Ferreira Tereza Cristina dos Reis 
Published: May 2024

Introdução: A COVID-19, doença causada pelo novo coronavírus SARS-CoV-2, surgiu no leste da Ásia e rapidamente tornou-se uma pandemia, possuindo como principais sintomas a febre, dispneia, tosse seca, cefaléia, cansaço e diarréia. Com o aumento da disseminação da doença, alterações do paladar (disgeusia) e do olfato (anosmia) foram identificadas como parte dos sintomas principais da infecção, atingindo cerca de 80% dos indivíduos. Somado a isso, a maior parte da população acometida procura atendimento em uma unidade básica de atendimento, enfatizando a importância da atenção básica em situações emergenciais, visto que esta possui ações que visam a proteção e promoção de saúde, permitindo desde a identificação de casos precocemente até o acompanhamento e evolução destes, sendo fundamental para contenção de uma pandemia. Objetivos: Identificar a prevalência da anosmia e disgeusia em pacientes vítimas de SARS- CoV- 2 no período de 2020 a 2022. Métodos: Trata-se de uma revisão narrativa da literatura, realizada por meio de uma busca nas bases de dados Scielo, LILACS, MEDLINE/Pubmed, PEDro e Biblioteca Virtual em Saúde, em portugûes e inglês, no período de 2020 a 2022. Resultado: Foram encontrados 114 artigos nas bases de dados Scielo, LILACS, MEDLINE/Pubmed, PEDro e Biblioteca Virtual em Saúde, onde foram excluídos 59 artigos por serem duplicados ou não se enquadrarem na temática da pesquisa, restando assim 55 artigos selecionados para análise . conforme mostra a tabela 1. Conclusão: Conclui-se que a anosmia na COVID-19 é mais prevalente no sexo feminino e em pacientes com menos de 50 anos. Juntamente com a disgeusia, é um importante sintoma precoce preditivo da doença.
Estimating Galaxy Parameters with Self-organizing Maps and the Effect of Missing Data
Authors: La Torre Valentina , Sajina Anna , Goulding Andy D. , Marchesini Danilo , Bezanson Rachel , Pearl Alan N. , Sodré Laerte 
Published: May 2024

Abstract
The current and upcoming large data volume galaxy surveys require the use of machine-learning techniques to maximize their scientific return. This study explores the use of Self-Organizing Maps (SOMs) to estimate galaxy parameters with a focus on handling cases of missing data and providing realistic probability distribution functions for the parameters. We train an SOM with a simulated mass-limited lightcone assuming a ugrizY
JHK

s
+IRAC data set, mimicking the Hyper Suprime-Cam Deep joint data set. For parameter estimation, we derive SOM likelihood surfaces considering photometric errors to derive total (statistical and systematic) uncertainties. We explore the effects of missing data, including which bands are particularly critical to the accuracy of the derived parameters. We demonstrate that the parameter recovery is significantly better when the missing bands are “filled in” rather than if they are completely omitted. We propose a practical method for such recovery of missing data.
TEMPLATES: Characterization of a Merger in the Dusty Lensing SPT0418–47 System
Authors: Cathey Jared , Gonzalez Anthony H. , Lower Sidney , Phadke Kedar A. , Spilker Justin , Aravena Manuel , Bayliss Matthew , Birkin Jack E. , Birrer Simon , Chapman Scott , Dahle Håkon , Hayward Christopher C. , Hezaveh Yashar , Hill Ryley , Hutchison Taylor A. , Kim Keunho J. , Mahler Guillaume , Marrone Daniel P. , Narayanan Desika , Navarre Alexander , Reuter Cassie , Rigby Jane R. , Sharon Keren , Solimano Manuel , Sulzenauer Nikolaus , Vieira Joaquin , Vizgan David 
Published: May 2024

Abstract
We present JWST and Atacama Large Millimeter/submillimeter Array (ALMA) imaging for the lensing system SPT0418−47, which includes a strongly lensed, dusty, star-forming galaxy at redshift z = 4.225 and an associated multiply imaged companion. The JWST NIRCam and MIRI imaging observations presented in this paper were acquired as part of the Early Release Science program Targeting Extremely Magnified Panchromatic Lensed Arcs and Their Extended Star formation (TEMPLATES). This data set provides robust mutiwavelength detections of stellar light in both the main (SPT0418A) and companion (SPT0418B) galaxies, while the ALMA detection of [C ii] emission confirms that SPT0418B lies at the same redshift as SPT0418A. We infer that the projected physical separation of the two galaxies is 4.42 ± 0.05 kpc. We derive total magnifications of μ = 29 ± 1 and μ = 4.1 ± 0.7 for SPT0418A and SPT0418B, respectively. We use both prospector and cigale to derive stellar masses. We find that SPT0418A has a stellar mass of

M

*

=

3.4

−
0.6

+
1.1

×

10

10

M

⊙

from prospector or M
* = 1.5 ± 0.3 × 1010
M
⊙ from cigale. The stellar mass ratio of SPT0418A and SPT0418B is roughly between 4 and 7 (

4.2

−
1.6

+
1.9

for prospector and 7.5 ± 3.7 for cigale). We see evidence of extended structure associated with SPT0418A that is suggestive of a tidal feature. These features, along with the close projected proximity, imply that the system is interacting. Interestingly, the star formation rates and stellar masses of both galaxies are consistent with the main sequence of star-forming galaxies at this epoch, indicating that this ongoing interaction has not noticeably elevated the star formation levels.