bluuebunny/crossref_metadata_2025_split huggingface dataset:
PatternJ: an ImageJ toolset for the automated and quantitative analysis of regular spatial patterns found in sarcomeres, axons, somites, and more
Authors: Baheux Blin Mélina , Loreau Vincent , Schnorrer Frank , Mangeol Pierre 
Published: June 2024

ABSTRACT
Regular spatial patterns are ubiquitous forms of organization in nature. In animals, regular patterns can be found from the cellular scale to the tissue scale, and from early stages of development to adulthood. To understand the formation of these patterns, how they assemble and mature, and how they are affected by perturbations, a precise quantitative description of the patterns is essential. However, accessible tools that offer in-depth analysis without the need for computational skills are lacking for biologists. Here, we present PatternJ, a novel toolset to analyze regular one-dimensional patterns precisely and automatically. This toolset, to be used with the popular imaging processing program ImageJ/Fiji, facilitates the extraction of key geometric features within and between pattern repeats in static images and time-lapse series. We validate PatternJ with simulated data and test it on images of sarcomeres from insect muscles and contracting cardiomyocytes, actin rings in neurons, and somites from zebrafish embryos obtained using confocal fluorescence microscopy, STORM, electron microscopy, and brightfield imaging. We show that the toolset delivers subpixel feature extraction reliably even with images of low signal-to-noise ratio. PatternJ's straightforward use and functionalities make it valuable for various scientific fields requiring quantitative one-dimensional pattern analysis, including the sarcomere biology of muscles or the patterning of mammalian axons, speeding up discoveries with the bonus of high reproducibility.
Hypoxic preconditioning promotes survival of human adipose derived mesenchymal stem cell via expression of prosurvival and proangiogenic biomarkers
Authors: Suryawan I Gde Rurus , Pikir Budi Susetyo , Rantam Fedik Abdul , Ratri Anudya Kartika , Nugraha Ricardo Adrian 
Published: June 2024

Background: Contributing factors for improved survival of human adipose derived mesenchymal stem cells (h-AMSCs) cultured through hypoxia preconditioning, in example apoptosis inhibition involving BCL2 and HSP27 expression, trigger signal expression (VEGF), SCF expression, OCT-4 expression, and CD44+ expression. The objective if this study was to explain the mechanism and role of hypoxic preconditioning and the optimal duration of hypoxic preconditioning exposure to improve survival of h-AMSCs. Methods: An experimental laboratory explorative study (in vitro) with hypoxic preconditioning in h-AMSCs cultures. This research was conducted through four stages. First, isolation of h-AMSCs culture from adipose tissue of patients. Second, the characterization of h-AMSCs from adipose tissue by phenotype (flowcytometry) through CD44+, CD90+ and CD45-expression before being pre-conditioned for hypoxic treatment. Third, the hypoxic preconditioning in h-AMSCs culture (in vitro) was performed with an oxygen concentration of 1% for 24, 48 and 72 hours. Fourth, observation of survival from h-AMSCs culture was tested on the role of CD44+, VEGF, SCF, OCT-4, BCL2, HSP27 with Flowcytometry and apoptotic inhibition by Tunnel Assay method. Results: The result of regression test showed that time difference had an effect on VEGF expression (p<0.001;β=-0.482) and hypoxia condition also influenced VEGF expression (p<0.001;β=0.774). The result of path analysis showed that SCF had effect on OCT-4 expression (p<0.001; β=0.985). The regression test results showed that time effects on HSP27 expression (p<0.001; β=0.398) and hypoxia precondition also affects HSP27 expression (p<0.001; β=0.847). Pathway analysis showed that BCL2 expression inhibited apoptosis (p=0.030; β=-0.442) and HSP27 expression also inhibited apoptosis (p<0,001;β=-0.487). Conclusion: Hypoxic preconditioning of h-AMSC culture has proven to increase the expression of VEGF, SCF, OCT-4, and BCL2 and HSP27. This study demonstrated and explained the existence of a new mechanism of increased h-AMSC survival in cultures with hypoxic preconditioning (O2 1%) via VEGF, SCF, OCT-4, BCL2, and HSP 27.
Short-term efficacy of microwave ablation in the treatment of liver cancer and its effect on immune function
Authors: Yao Li-Jun , Zhu Xiao-Ding , Zhou Liu-Min , Zhang Li-Li , Liu Na-Na , Chen Min , Wang Jia-Ying , Hu Shao-Jun 
Published: June 2024

BACKGROUND
Hepatectomy is the first choice for treating liver cancer. However, inflammatory factors, released in response to pain stimulation, may suppress perioperative immune function and affect the prognosis of patients undergoing hepatectomies.
AIM
To determine the short-term efficacy of microwave ablation in the treatment of liver cancer and its effect on immune function.
METHODS
Clinical data from patients with liver cancer admitted to Suzhou Ninth People’s Hospital from January 2020 to December 2023 were retrospectively analyzed. Thirty-five patients underwent laparoscopic hepatectomy for liver cancer (liver cancer resection group) and 35 patients underwent medical image-guided microwave ablation (liver cancer ablation group). The short-term efficacy, complications, liver function, and immune function indices before and after treatment were compared between the two groups.
RESULTS
One month after treatment, 19 patients experienced complete remission (CR), 8 patients experienced partial remission (PR), 6 patients experienced stable disease (SD), and 2 patients experienced disease progression (PD) in the liver cancer resection group. In the liver cancer ablation group, 21 patients experienced CR, 9 patients experienced PR, 3 patients experienced SD, and 2 patients experienced PD. No significant differences in efficacy and complications were detected between the liver cancer ablation and liver cancer resection groups (P > 0.05). After treatment, total bilirubin (41.24 ± 7.35 vs 49.18 ± 8.64 μmol/L, P < 0.001), alanine aminotransferase (30.85 ± 6.23 vs 42.32 ± 7.56 U/L, P < 0.001), CD4+ (43.95 ± 5.72 vs 35.27 ± 5.56, P < 0.001), CD8+ (20.38 ± 3.91 vs 22.75 ± 4.62, P < 0.001), and CD4+/CD8+ (2.16 ± 0.39 vs 1.55 ± 0.32, P < 0.001) were significantly different between the liver cancer ablation and liver cancer resection groups.
CONCLUSION
The short-term efficacy and safety of microwave ablation and laparoscopic surgery for the treatment of liver cancer are similar, but liver function recovers quickly after microwave ablation, and microwave ablation may enhance immune function.
High-grade serous carcinoma of the fallopian tube in a young woman with chromosomal 4q abnormality: A case report
Authors: Zhang Kai-Cheng , Chu Shao-Yin , Ding Dah-Ching 
Published: June 2024

BACKGROUND
Few studies have reported an association between an increased risk of acquiring cancers and survival in patients with 4q deletion syndrome. This study presents a rare association between chromosome 4q abnormalities and fallopian tube high-grade serous carcinoma (HGSC) in a young woman.
CASE SUMMARY
A 35-year-old woman presented with acute dull abdominal pain and a known chromosomal abnormality involving 4q13.3 duplication and 4q23q24 deletion. Upon arrival at the emergency room, her abdomen appeared ovoid and distended with palpable shifting dullness. Ascites were identified through abdominal ultrasound, and computed tomography revealed an omentum cake and an enlarged bilateral adnexa. Blood tests showed elevated CA-125 levels. Paracentesis was conducted, and immunohistochemistry indicated that the cancer cells favored an ovarian origin, making us suspect ovarian cancer. The patient underwent debulking surgery, which led to a diagnosis of stage IIIC HGSC of the fallopian tube. Subsequently, the patient received adjuvant chemotherapy with carboplatin and paclitaxel, resulting in stable current condition.
CONCLUSION
This study demonstrates a rare correlation between a chromosome 4q abnormality and HGSC. UBE2D3 may affect crucial cancer-related pathways, including P53, BRCA, cyclin D, and tyrosine kinase receptors, thereby possibly contributing to cancer development. In addition, ADH1 and DDIT4 may be potential influencers of both carcinogenic and therapeutic responses.
Parathyroid carcinoma located in the thyroid gland: A case report
Authors: Gui Shu-Yan , Zhang Chang-Ning , Ling Li , Tang Ruo-Xi , Yang Jing 
Published: June 2024

BACKGROUND
Parathyroid carcinoma (PC) is a difficult-to-diagnose rare disease with low incidence. Relatively accurate preoperative diagnosis is very important in choosing surgical methods and patient prognosis.
CASE SUMMARY
This study reported the clinical diagnosis and treatment of a rare patient with PC located in the thyroid gland and provided a case reference for the diagnosis and treatment of PC. A case of a 64-year-old male patient who presented to our hospital with systemic muscle and joint pain and palpitations is outlined. Subsequently, the patient was admitted to the Department of Nephrology for the treatment of “multiple myeloma nephropathy pending investigation”. The patient was diagnosed with “primary hyperparathyroidism and hypercalcemic crisis” using thyroid color ultrasound.
CONCLUSION
The intraoperative frozen section report considered the parathyroid tumor. Surgical tumor resection was promptly performed, and the diagnosis of PC was confirmed.
Vomiting-induced pharyngeal perforation during bowel preparation for colonoscopy: A case report
Authors: Kim Geonhee , Lee Won Hyuk , Kang Seokin , Moon Jung Rock , Lee Yoon Suk , Son Jun Hyuk , Kim Nam-Hoon , Kim Jong Wook 
Published: June 2024

BACKGROUND
Effective bowel cleansing is essential for a successful colonoscopy. Laxatives, such as polyethylene glycol, are commonly used for bowel preparation. Vomiting is a frequent complication during bowel preparation, and forceful vomiting can potentially lead to esophageal perforation, as reported in several previous cases. However, pharyngeal perforation during bowel preparation has not been previously documented. Here, we present a case of pharyngeal perforation induced by forceful vomiting during bowel preparation.
CASE SUMMARY
A 38-year-old man with a history of hypertension, dyslipidemia, diabetes mellitus, and end-stage renal disease on hemodialysis was admitted for evaluation of recurrent abdominal pain. The patient complained of sudden pain in the neck, throat, and anterior chest following forceful vomiting during bowel preparation. Physical examination revealed crepitus under the skin of the neck and anterior chest on palpation, and upper gastrointestinal endoscopy revealed pharyngeal perforation. The perforation site was located above the upper esophageal sphincter, which distinguished it from Boerhaave’s syndrome. Conservative medical management was chosen after consultation with a thoracic surgeon and an otolaryngologist, considering the patient's mild symptoms, stable vital signs, and the small size of the lesion; the perforation resolved without endoscopic or surgical intervention. The patient was discharged from hospital two weeks after the perforation.
CONCLUSION
Despite its rarity, pharyngeal perforation should be considered a potential complication of bowel preparation for colonoscopy.
Multiwavelength lateral-shearing spherical interferometry for a large-aperture dichroic mirror
Authors: Cai Zhiqian , Zhang Junyong , Yang Pengqian , Ma Xiaojun , Shen Weixing , Zhang Xuejie , Zhu Jianqiang 
Published: June 2024

Large-aperture dichroic mirrors play an important role in various high-power laser systems and have been proposed for multiwavelength wavefront measurements. In this study, we first developed the theory of multiwavelength lateral-shearing spherical interferometry to achieve signal separation under coaxial conditions. Subsequently, an experiment on wavefront sensing from one interferogram was successfully performed on a dichroic mirror with a diameter of 350 mm. The results are consistent with those of the Zygo interferometer, indicating the proposed method’s potential applications in high-order harmonic detection, multiwavelength interferometric imaging, and laser plasma diagnosis.
Espaço físico escolar e o processo de criação na arquitetura: a percepção do aluno na pandemia
Authors: Almeida Barbosa Luíza Chiarelli , Mendes Godoy Rosa Maria 
Published: June 2024

Este trabalho tem como objetivo compreender o impacto do afastamento do espaço físico escolar durante a pandemia, para os alunos de Arquitetura e Urbanismo, no processo de criação nas disciplinas de projeto de arquitetura, urbanismo e paisagismo em uma instituição de ensino superior particular, em Curitiba. Aplicou-se questionário, mediado por TCLE – Termo de Consentimento Livre e Esclarecido, respondido pelos discentes, e fez-se entrevista estruturada com o gestor. A partir disso, evidenciou-se a importância do espaço físico escolar para o estímulo dos processos criativos. Os principais efeitos do distanciamento físico foram interpretados, de modo geral, negativamente pelos alunos, professores e coordenação. Porém, as mudanças estabelecidas pelo Ensino Remoto Emergencial (ERE) concretizaram o ensino on-line das disciplinas de projeto, influenciando no surgimento de cursos de graduação nos moldes de Educação a Distância (EaD), reconhecidos pelo Ministério da Educação (MEC), mas ainda questionados e desaprovados pelo Conselho de Arquitetura e Urbanismo do Brasil (CAU BR). Nessa investigação, procurou-se contribuir para as discussões sobre o desenvolvimento criativo nos modos remotos recentes, nas possíveis situações semelhantes que possam acontecer no futuro, ou no refinamento de instrumentos educacionais. Mesmo sem esgotar o assunto, refletiu-se sobre as dificuldades da expressão criativa por meio do sistema remoto para os alunos, e compreendeu-se a significação dos espaços físicos escolares para as disciplinas de projeto.
From Traditional Narratives to Literary Innovation: A Quantitative Analysis of Virginia Woolf’s Stylistic Evolution
Authors: Huang Lei 
Published: June 2024

Abstract
Virginia Woolf, one of the most renowned modernist British writers, left an enduring imprint on the literary landscape with her distinct and influential writing style. Her literary journey is characterized by an evolution, transitioning from conformity with Victorian and Edwardian literary conventions to pioneering a groundbreaking and experimental approach, notably employing the innovative technique of stream of consciousness. This investigation seeks to identify and quantify this transformation. To achieve this goal, I curated two distinct corpora representing Woolf’s early and later works and meticulously processed them using the multi-dimensional analysis tagger. Subsequently, a battery of independent samples was conducted to compare six distinct dimensions and then 67 linguistic features between the two corpora. This study uncovers a discernible evolution in Woolf’s writing style, with her later works displaying a lessened degree of persuasiveness, abstraction, and online communicativeness, and an enhanced degree of personal involvement and subjectivity. Our findings not only enrich our comprehension of Woolf’s literary development and accomplishments but also shed light on the broader context of the modernist movement and its profound influence on the modernist literary style.
Gender agreement in Italian compounds with capo-
Authors: Lami Irene , Micheli Maria Silvia , Radimský Jan , van de Weijer Joost 
Published: June 2024

Abstract
Gender inflection for animated nouns in Italian presents challenges influenced by societal pressures and linguistic structure, especially in morphologically complex words like compounds. The study investigates gender inflection distribution in compounds with capo- compared to other nouns (i.e., occupations traditionally performed by women, by men, and the word capo in isolation), exploring the interplay of social, etymological and morphological factors. 192 native Italian speakers inflected masculine nouns to feminine forms after hearing the stimulus. Results reveal that respondents’ attitudes towards gender-fair language significantly determine the use of feminine, indicating a complex interplay between linguistic structures and social perceptions. Despite historical resistance, the word capa in isolation shows increasing acceptance, challenging entrenched norms. In compounds, capo- element’s gender inflection appears more resistant due to morphological complexity, with an interaction with number. This study advances our understanding of gender inflection, with implications for broader conversations about gender representation and language inclusivity.
Assessment of the fetal thymic-thoracic ratio in pregnant women with intrahepatic cholestasis: a prospective case-control study
Authors: Agaoglu Zahid , Tanacan Atakan , Bozkurt Ozdal Burcu , Basaran Ezgi , Serbetci Hakkı , Ozturk Agaoglu Merve , Okutucu Gulcan , Kara Ozgur , Sahin Dilek 
Published: June 2024

Abstract

Objectives
To examine the fetal thymic-thoracic ratio (TTR) in intrahepatic cholestasis of pregnancy (ICP).

Methods
This prospective case-control study was conducted in a single tertiary center. The sample consisted of 86 pregnant women at 28–37 weeks of gestation, including 43 women with ICP and 43 healthy controls. TTR was calculated for each patient using the anterior-posterior measurements of the thymus and intrathoracic mediastinal measurements.

Results
The median TTR value was found to be smaller in the ICP group compared to the control group (0.32 vs. 0.36, p<0.001). The ICP group had a greater rate of admission to the neonatal intensive care unit (NICU) (p<0.001). Univariate regression analysis revealed that lower TTR values increased the possibility of NICU admission six times (95 % confidence interval: 0.26–0.39, p=0.01). A statistically significant negative correlation was detected between TTR and the NICU requirement (r: −0.435, p=0.004). As a result of the receiver operating characteristic analysis, in predicting NICU admission, the optimal cut-off value of TTR was determined to be 0.31 with 78 % sensitivity and 67 % specificity (area under the curve=0.819; p<0.001).

Conclusions
We determined that the fetal TTR may be affected by the inflammatory process caused by the maternal-fetal immune system and increased serum bile acid levels in fetal organs in the presence of ICP. We consider that TTR can be used to predict adverse pregnancy outcomes in patients with ICP.
Evaluation for serum glucose standardization in clinical laboratories of Southern China by consecutive 6 years proficiency testing based on JCTLM-recommended reference methods
Authors: Lin Xueying , Yan Qiaofang , Du Yuanyuan , Wang Jianbing , Huang Di , Yan Jun , Zhan Min , Zhang Pengwei , Cheng Jingyu , Zhang Qiaoxuan , Huang Xianzhang , Han Liqiao 
Published: June 2024

Abstract

Objectives
The accuracy of blood glucose measurement in clinical laboratories is vital for diabetes diagnosis. Trueness Verification Plan was carried out and analyzed for evaluating the standardization of serum glucose among clinical laboratories.

Methods
Trueness verification samples were distributed to clinical laboratories for three days measurement, and their target values were assigned by two certified reference laboratories. The relative bias, coefficient of variation (CV), and total error (TE) for each clinical laboratory were calculated and analyzed. Moreover, the Six Sigma metrics and Quality Goal Index were utilized to reflect the measurement quality of the clinical laboratories.

Results
The pass rates evaluated by bias, CV, and TE ranged from 45.2 % to 64.8 %, 96.8 %–98.9 %, and 83.9 %–97.1 % over the six years. The matched systems used in clinical laboratories demonstrated better accuracy than the un-matched systems. The pass rate by bias of hexokinase method is 53.1 %–78.6 %, while the glucose oxidase method is 29.2 %–52.2 %. Overall, 74.2 %–85.7 % of clinical laboratories achieved an acceptable level (both σ>3), and 35.2 %–61.4 % of laboratories reached a “world-class” level (both σ>6).

Conclusions
The quality for serum glucose measurement has been greatly improved. However, standardization among clinical systems still needs to be further promoted.
Quantification of collective signalling in time-lapse microscopy images
Authors: Dobrzyński Maciej , Grädel Benjamin , Gagliardi Paolo Armando , Pertz Olivier 
Published: June 2024

Abstract
Live-cell imaging of fluorescent biosensors has demonstrated that space-time correlations in signalling of cell collectives play an important organisational role in morphogenesis, wound healing, regeneration, and maintaining epithelial homeostasis. Here, we demonstrate how to quantify one such phenomenon, namely apoptosis-induced ERK activity waves in the MCF10A epithelium. We present a protocol that starts from raw time-lapse fluorescence microscopy images and, through a sequence of image manipulations, ends with ARCOS, our computational method to detect and quantify collective signalling. We also describe the same workflow in the interactive napari image viewer to quantify collective phenomena for users without prior programming experience. Our approach can be applied to space-time correlations in cells, cell collectives, or communities of multicellular organisms, in 2D and 3D geometries.
Crystal structure of dichlorido-bis(1-[(2-ethyl-benzimidazole-1-yl)methyl]-1H–benzotriazole) cadmium(II), C32H32CdN10OCl2
Authors: Yang Jing , Zhou Tan-Peng , Cao Meng-Meng , Wang Xia 
Published: June 2024

Abstract
C32H32CdN10OCl2, monoclinic, C2/c (no. 15), a = 28.820(3) Å, b = 13.1075(2) Å, c = 16.7266(19) Å, β = 149.29(3)°, Z = 4, V = 3226.9(15) Å3, R

gt
(F) = 0.0341, wR

ref
(F
2) = 0.0893, T = 293(2) K.
Two new enid land snails from Jiuzhaigou County, Sichuan Province, China (Gastropoda: Stylommatophora: Enidae)
Authors: Chen Zhong-Guang , Hu Hong-Fei , Dai Yu-Ting , Wang Ping , Jiang Jiao , Wu Xiao-Ping , Ouyang Shan 
Published: June 2024

AbstractTwo new land snail species of the family Enidae, Serina xirong Chen, Dai, Wu & Ouyang sp. n. and Turanena jiuzhaigouensis Chen, Dai, Wu & Ouyang sp. n., are described from Jiuzhaigou County, Sichuan Province, China based on conchological morphology. Serina xirong can be distinguished from congeneric species by its ribbed teleoconch, and Turanena jiuzhaigouensis can be distinguished from congeneric species by its micro, conic, weakly keeled shell without color stripes before body whorl. This discovery further enhances our understanding of the enid species diversity in Sichuan Province.