bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of N‐Acetylgalactosamine in Carbohydrates of Xenopus laevis Testis
Authors: Valbuena Galder , Alonso Edurne , Díaz‐Flores Lucio , Madrid Juan Francisco , Sáez Francisco José 
Published: December 2010

AbstractIdentification of glycans in amphibian testis has shown the existence of N‐acetylgalactosamine (GalNAc)‐containing carbohydrates. Labeling of the sperm acrosome with GalNAc‐binding lectins has allowed the identification of GalNAc‐containing glycans in this organelle. Futhermore, this specific labeling of the acrosome has allowed the study of acrosomal biogenesis by lectin histochemistry. However, the testis of Xenopus laevis has never been analyzed by lectin histochemistry to locate GalNAc‐containing glycoconjugates. The aim of this work was to elucidate the expression of GalNAc in glycoconjugates of Xenopus testis using five specific lectins. The results showed that most of the lectins labeled the interstitium with variable intensity. However, labeling of the different spermatogenetic germ cell types showed different labeling patterns. Some lectins produced weak or very weak staining in germ cells, for example, horse gram Dolichos biflorus agglutinin, which labeled most of the germ cell types, and lima bean Phaseolus lunatus agglutinin, which weakly labeled only spermatogonia, but did not stain other germ cells. By contrast, Maclura pomifera lectin (MPL) moderately labeled all germ cell types, except mature sperm. Labeling with other lectins was seen only at later stages, suggesting variations involved in the spermatogenetic development. Thus, snail Helix pomatia agglutinin labeled spermatids, but neither spermatogonia nor spermatocytes, while soybean Glycine max agglutinin (SBA) labeled from preleptotene spermatocytes to later stages. The periphery of the acrosome was labeled with MPL and SBA, but no specific labeling of the acrosomal content was seen with any lectin. Thus, the GalNAc‐binding lectins that have been used as acrosomal markers in some amphibians cannot be used in Xenopus testis, suggesting that acrosomal glycoconjugates in amphibians are species specific. Anat Rec, 2011. © 2010 Wiley‐Liss, Inc.
Proportional multiple‐integral observer design for continuous‐time descriptor linear systems
Authors: Wu Ai‐Guo , Duan Guang‐Ren , Liu Wanquan 
Published: December 2010

AbstractIn this paper, a new type of proportional multiple‐integral observer is proposed for continuous‐time descriptor linear systems. A parametric design approach for the proposed observers is presented. Based on a complete general parametric solution to the generalized Sylvester‐observer matrix equation, complete parameterizations for all the observer gains are established in terms of four parameter matrices. The proposed approach can offer full design freedom, and has great potential in applications. A numerical example is given to show the design procedure and the effectiveness of the proposed approach.Copyright © 2010 John Wiley and Sons Asia Pte Ltd and Chinese Automatic Control Society
Pharmacokinetics of dietary cancer chemopreventive compound dibenzoylmethane in rats and the impact of nanoemulsion and genetic knockout of Nrf2 on its disposition
Authors: Lin Wen , Hong Jin‐Liern , Shen Guoxiang , Wu Rachel T. , Wang Yuwen , Huang Mou‐Tuan , Newmark Harold L. , Huang Qingrong , Khor Tin Oo , Heimbach Tycho , Kong Ah‐Ng 
Published: December 2010

AbstractThe pharmacokinetic disposition of a dietary cancer chemopreventive compound dibenzoylmethane (DBM) was studied in male Sprague‐Dawley rats after intravenous (i.v.) and oral (p.o.) administrations. Following a single i.v. bolus dose, the mean plasma clearance (CL) of DBM was low compared with the hepatic blood flow. DBM displayed a high volume of distribution (Vss). The elimination terminal t1/2 was long. The mean CL, Vss and AUC0−∞/dose were similar between the i.v. 10 and 10 mg/kg doses. After single oral doses (10, 50 and 250 mg/kg), the absolute oral bioavailability (F*) of DBM was 7.4%–13.6%. The increase in AUC was not proportional to the oral doses, suggesting non‐linearity. In silico prediction of oral absorption also demonstrated low DBM absorption in vivo. An oil‐in‐water nanoemulsion containing DBM was formulated to potentially overcome the low F* due to poor water solubility of DBM, with enhanced oral absorption. Finally, to examine the role of Nrf2 on the pharmacokinetics of DBM, since DBM activates the Nrf2‐dependent detoxification pathways, Nrf2 wild‐type (+/+) mice and Nrf2 knockout (−/−) mice were utilized. There was an increased systemic plasma exposure of DBM in Nrf2 (−/−) mice, suggesting that the Nrf2 genotype could also play a role in the pharmacokinetic disposition of DBM. Taken together, the results show that DBM has low oral bioavailability which could be due in part to poor water solubility and this could be overcome by a nanotechnology‐based drug delivery system and furthermore the Nrf2 genotype could also play a role in the pharmacokinetics of DBM. Copyright © 2010 John Wiley & Sons, Ltd.
Engineering of baculovirus vectors for the manufacture of virion‐free biopharmaceuticals
Authors: Marek Martin , van Oers Monique M. , Devaraj Feana Francis , Vlak Just M. , Merten Otto‐Wilhelm 
Published: December 2010

AbstractA novel baculovirus‐based protein expression strategy was developed to produce recombinant proteins in insect cells without contaminating baculovirus virions. This novel strategy greatly simplifies the downstream processing of biopharmaceuticals produced in insect cells. The formation of these virions is prevented by deletion of a baculovirus gene essential for virion formation. The deletion is trans‐complemented in a transgenic insect cell line in which the baculovirus seed stock is produced. The Autographa californica multicapsid nucleopolyhedrovirus vp80 gene was selected for this purpose, as absence of VP80 prevented the formation of budded virus as well as occlusion‐derived virus, while foreign gene expression was not affected. Sf9 insect cells were engineered to functionally complement the vp80 deletion in the expression vector virus during seed stock production. The trans‐complemented vp80‐deletion baculovirus seed produced an amount of recombinant protein similar to that produced with conventional baculovirus vectors but without contaminating virions. This novel expression method obviates the need to purify the virions away from the biopharmaceuticals. Bioeng. 2011; 108:1056–1067. © 2010 Wiley Periodicals, Inc.
Three hours of perfusion culture prior to 28 days of static culture, enhances osteogenesis by human cells in a collagen GAG scaffold
Authors: Keogh Michael B. , Partap Sonia , Daly Jacqueline S. , O'Brien Fergal J. 
Published: December 2010

AbstractIn tissue engineering, bioreactors can be used to aid in the in vitro development of new tissue by providing biochemical and physical regulatory signals to cells and encouraging them to undergo differentiation and/or to produce extracellular matrix prior to in vivo implantation. This study examined the effect of short term flow perfusion bioreactor culture, prior to long‐term static culture, on human osteoblast cell distribution and osteogenesis within a collagen glycosaminoglycan (CG) scaffold for bone tissue engineering. Human fetal osteoblasts (hFOB 1.19) were seeded onto CG scaffolds and pre‐cultured for 6 days. Constructs were then placed into the bioreactor and exposed to 3 × 1 h bouts of steady flow (1 mL/min) separated by 7 h of no flow over a 24‐h period. The constructs were then cultured under static osteogenic conditions for up to 28 days. Results show that the bioreactor and static culture control groups displayed similar cell numbers and metabolic activity. Histologically, however, peripheral cell‐encapsulation was observed in the static controls, whereas, improved migration and homogenous cell distribution was seen in the bioreactor groups. Gene expression analysis showed that all osteogenic markers investigated displayed greater levels of expression in the bioreactor groups compared to static controls. While static groups showed increased mineral deposition; mechanical testing revealed that there was no difference in the compressive modulus between bioreactor and static groups. In conclusion, a flow perfusion bioreactor improved construct homogeneity by preventing peripheral encapsulation whilst also providing an enhanced osteogenic phenotype over static controls. Bioeng. 2011; 108:1203–1210. © 2010 Wiley Periodicals, Inc.
Randomized controlled trial of ultrasonic dissection versus standard surgical technique in open left hemicolectomy or total gastrectomy
Authors: Wilhelm D , Szabo M , Glass F , Schuhmacher C , Friess H , Feussner H 
Published: December 2010

Abstract

Background
Ultrasonic dissection devices have been designed for use in open surgery but it is not certain how they compare with standard surgical techniques.

Methods
This was a multicentre randomized controlled trial comparing ultrasonic dissection with the traditional surgical technique for haemostasis and dissection during left hemicolectomy and total gastrectomy. The primary endpoint was duration of operation; secondary endpoints were blood loss and other intraoperative parameters, and patient outcomes. Performance of the two techniques was rated by surgeons and assistants on a ten-point Likert scale.

Results
The analysis included 100 patients in the ultrasonic and 101 in the conventional dissection group. Patient demographics, and clinical and tumour-related parameters were similar in the two groups. There was no significant difference in duration of operation (mean 170 and 178 min in ultrasonic and conventional groups respectively; P = 0·405). Nor were there significant differences in intraoperative blood loss (median 350 and 400 ml respectively; P = 0·882), other intraoperative parameters, oncological or functional outcome. The ultrasonic dissector device was rated one point higher than conventional techniques by the surgeons.

Conclusion
Use of the ultrasonic dissector in open total gastrectomy and hemicolectomy had no impact on the overall operating time or other endpoints studied. Surgeons preferred the ultrasonic device for dissection. Registration number: ISRCTN97779420 (http://www.controlled-trials.com).
Recruiting and mentoring minority faculty members
Authors: Vega William , Yglesias Kenneth , Murray John P. 
Published: December 2010

AbstractFor the foreseeable future, community colleges must seriously address hiring, retaining, and facilitating upward mobility for faculty members. Moreover, they should recruit a faculty corps that is more reflective of both the students they serve and the demographics of their college service area. It is critical for community colleges to employ and retain a diverse faculty.
Regiocontrolled Halohydroxylations of Bicyclic Vinylidenecyclopropanes: A Versatile Strategy for the Construction of Diverse Highly Functionalized Carbocyclic Scaffolds
Authors: Su Chenliang , Cao Jian , Huang Xin , Wu Luling , Huang Xian 
Published: December 2010

AbstractHighly regioselective halohydroxylations of bicyclic vinylidenecyclopropanes that lead to four types of products 2, 3, 4, and 6 were developed. The halohydroxylation reaction occurs at room temperature to give rise to ring‐keeping products vinylbicyclo[(n+2).1.0]alkanols 2 in 55–90 % yields with excellent regio‐ and diastereoselectivity; the reaction of bicyclic vinylidenecyclopropanes with 2.0 equiv of N‐bromosuccinimide (NBS) at 100 °C affords alkylidenebicyclo[(n+2).2.0]alkanones 3 in 48–75 % yields by means of further proximal cleavage of the cyclopropane ring. The structures of both types of compounds 2 and 3 have been elucidated by X‐ray crystal diffraction. An interesting sequential reaction that consists of a couple electrophilic addition and elimination reactions was developed when the reaction of bicyclic vinylidenecyclopropanes with N‐halosuccinimide (NXS; 3.0 equiv) was performed under the specified conditions to furnish a variety of divinyl ketones 4 by means of proximal cleavage of the cyclopropane ring. In addition, vinylidenecyclopropanes that bore one aryl group at the cyclopropyl ring reacted with NBS or I2 at room temperature, thereby producing the corresponding divinyl ketones 4 in moderate to good yields with excellent E selectivity. Unexpectedly, 2‐vinylic cyclohex‐2‐enols 6 were generated through a very different distal CC bond cleavage of the cyclopropane due to the significant ring‐size effect. Possible mechanisms are proposed on the basis of the obtained intermediates.
Role of Steric Effects in Protein‐Directed Enediyne Cycloaromatization of Neocarzinostatin
Authors: Chi Hung‐Wen , Chien Yi‐Chih , Liu Cheng‐Yun , Tseng Chin‐Jui , Lee Yan‐Jiun , Chan Ja‐Lin , Chu Yu‐Ru , Chin Der‐Hang 
Published: December 2010

AbstractThe antibiotic neocarzinostatin comprises a carrier protein with a well‐defined cavity for accommodating an active enediyne chromophore. The protein has two disulfides, one (Cys37–Cys47) lies on the cavity bottom and the other (Cys88–Cys93) in a constrained short loop. When the chromophore is not bound to the protein, a thiol‐induced cycloaromatization of the enediyne into a tetrahydroindacene derivative is responsible for the potent antitumor activity. When it is protein‐bound, the protein diverts the cycloaromatization pathway to form a distinct hydroxyisochromene‐type product. How the protein directs the enediyne chemistry is an interesting puzzle, and various suggestions have been proposed in the past. We screened more than fifty thiols and manipulated conditions to locate reaction features and search for factors that could influence the protein directing strength. Thiol‐ and oxygen‐concentration‐dependence studies suggested that disulfides, which maintain the steric rigidity of the protein, could play a key role in diverting the cycloaromatization pathway. For direct proofs, we made mutations at each of the two disulfides by replacing sulfur atoms with oxygen. Circular dichroism and two‐dimensional NMR spectroscopy studies suggested that the mutations changed neither the protein conformation nor the ligand interactions. Analyses of the thiol‐induced cycloaromatization revealed that rupture of Cys37–Cys47 made the protein almost completely lose its chemical directing ability, whereas rupture of Cys88–Cys93 had only a minor influence. The results demonstrated that the steric rigidity of the binding cavity, but not necessary the whole protein, played an important role in the protein‐directed mechanism.
ChemInform Abstract: First‐Principles Study of Defective and Nonstoichiometric Sr2FeMoO6.
Authors: Mishra Rohan , Restrepo Oscar D. , Woodward Patrick M. , Windl Wolfgang 
Published: December 2010

AbstractThe influence of disorder and stoichiometry‐breaking point defects on the structural and magnetic properties of Sr2FeMoO6 is studied by electronic structure calculations within the spin‐polarized GGA+U approach.
ChemInform Abstract: The Novel Arsenate Na4Co7‐xAl2/3x(AsO4)6 (x = 1.37): Crystal Structure, Charge‐Distribution and Bond‐Valencesum Investigations.
Authors: Marzouki Riadh , Guesmi Abderrahmen , Driss Ahmed 
Published: December 2010

AbstractSingle crystals of the new title compound are prepared by solid state reaction of Na2CO3, Co(OAc)2·4H2O, and As2O3 in air (675→1153 K, 3 d).
ChemInform Abstract: Investigation of the Chromic Phase Transition of CuMo0.9W0.1O4 Induced by Surface Protonation.
Authors: Gaudon M. , Riml C. , Turpain A. , Labrugere C. , Delville M. H. 
Published: December 2010

AbstractThe chromic phase transition from the high‐temperature/low‐pressure form of CuMo0.9W0.1O4 (γ‐phase, red‐brown) to the low‐temperature/high‐pressure form (α‐phase, green) is induced at 25 °C by simple immersion of the powder in an acidic aqueous solution.
ChemInform Abstract: Carbon Nanotube‐Amorphous FePO4 Core—Shell Nanowires as Cathode Material for Li Ion Batteries.
Authors: Kim Sung‐Wook , Ryu Jungki , Park Chan Beum , Kang Kisuk 
Published: December 2010

AbstractThe title nanowires are prepared by functionalizing multi‐walled carbon nanotubes with carboxyl groups followed by mineralizing them through sequential adsorption of Fe3+ and PO43‐ ions onto the carbon nanotube surface in aqueous solution under ambient conditions.
ChemInform Abstract: Synthesis, Crystal Structure, and Properties of MgxB50C8 or Mgx(B12)4(CBC)2 (C2)2 (x = 2.4—4).
Authors: Adasch Volker , Schroeder Melanie , Kotzott Dominik , Ludwig Thilo , Vojteer Natascha , Hillebrecht Harald 
Published: December 2010

AbstractSingle crystals of the title compounds are synthesized from the elements in a Cu/Mg melt (Ar, 1573—1673 K, 6—30 h) and characterized by single crystal XRD, microhardness measurements, IR, Raman, and UV/VIS spectroscopy.
ChemInform Abstract: Copper‐Catalyzed Enantioselective Propargylic Amination of Propargylic Esters with Amines: Copper—Allenylidene Complexes as Key Intermediates.
Authors: Hattori Gaku , Sakata Ken , Matsuzawa Hiroshi , Tanabe Yoshiaki , Miyake Yoshihiro , Nishibayashi Yoshiaki 
Published: December 2010

AbstractUnder optimized conditions, a wide variety of secondary amines undergo the title reaction with propargylic acetates with up to 98% enantioselectivity.
ChemInform Abstract: Rhodium‐Catalyzed Oxidative Cycloaddition of Benzamides and Alkynes via C—H/N—H Activation.
Authors: Hyster Todd K. , Rovis Tomislav 
Published: December 2010

AbstractIn the presence of a copper(II) salt as oxidant, benzamides as well as heterocyclic carboxamides undergo the title reaction with a variety of internal alkynes to give isoquinolinones or the corresponding heteroaryl‐fused pyridine analogues.
ChemInform Abstract: Preparation of 5‐Cyano‐4,6‐dimethyl‐2H‐pyran‐2‐one and 3‐Cyano‐5‐methoxy‐4‐methyl‐5H‐furan‐2‐one via a One‐Pot, Domino‐Knoevenagel Process.
Authors: Dawadi Prativa B. S. , Lugtenburg Johan 
Published: December 2010

AbstractAmmonium acetate / acetic acid‐catalyzed Knoevenagel reactions are studied.
ChemInform Abstract: A Divergent Approach to Highly Substituted Benzothiepinones and to 2,3‐Dihydrothieno[2,3‐b]thiopyran‐4‐ones.
Authors: Boutillier Peter , Quiclet‐Sire Beatrice , Zafar Syeda Nahid , Zard Samir Z. 
Published: December 2010

AbstractA variety of highly functionalized benzothiepinones, including libraries of complex aza‐bridged derivatives, and highly functionalized 2,3‐dihydrothieno[2,3‐b]thiopyran‐4‐ones are prepared by combination of the radical and ionic reactions of xanthates.
ChemInform Abstract: Investigation of the Mechanism of C(sp3)—H Bond Cleavage in Pd(0)‐Catalyzed Intramolecular Alkane Arylation Adjacent to Amides and Sulfonamides.
Authors: Rousseaux Sophie , Gorelsky Serge I. , Chung Benjamin K. W. , Fagnou Keith 
Published: December 2010

AbstractA Pd(0)‐catalyzed intramolecular arylation reaction at aliphatic positions adjacent to a nitrogen atom in amides and sulfonamides is described.
ChemInform Abstract: Dehydrogenative Functionalization of C(sp3)—H Bonds Adjacent to a Heteroatom Mediated by Oxoammonium Salts.
Authors: Richter Heinrich , Garcia Mancheno Olga 
Published: December 2010

AbstractThis methodology provides a safe and easy way to prepare α‐functionalized ethers and amines using oxoammonium TEMPO salt as easy‐to‐handle nontoxic oxidant.
ChemInform Abstract: Catalytic Coupling of N‐Benzylic Sulfonamides with Silylated Nucleophiles at Room Temperature.
Authors: Yang Bai‐Ling , Tian Shi‐Kai 
Published: December 2010

AbstractTriflimide acts as acidic catalyst for the unprecedented coupling reaction of benzylic sulfonamides with silylated nucleophiles through sp3 carbon—nitrogen bond cleavage.
ChemInform Abstract: Heterogeneous Chiral Copper Complexes of Amino Alcohol for Asymmetric Nitroaldol Reaction.
Authors: Mayani Vishal J. , Abdi Sayed H. R. , Kureshy Rukhsana I. , Khan Noor‐ul H. , Das Anjan , Bajaj Hari C. 
Published: December 2010

AbstractThe Cu complex together with the imine shown represents an efficient system which allows formation of a number of nitro alcohols in good yields with high enantioselectivity.
ChemInform Abstract: Chemoselective Reductive Cross‐Coupling of 1,5‐Diene‐3‐ols with Alkynes: A Facile Entry to Stereodefined Skipped Trienes.
Authors: Diez Peter S. , Micalizio Glenn C. 
Published: December 2010

AbstractDouble substitution of the starting alkenes is crucial for the reaction to proceeds selectively.
ChemInform Abstract: Stereoselective α‐Aminoallylation of Aldehydes with Chiral tert‐Butanesulfinamides and Allyl Bromides.
Authors: Gonzalez‐Gomez Jose C. , Medjahdi Mohamed , Foubelo Francisco , Yus Miguel 
Published: December 2010

AbstractIt provides a straightforward method to prepare optically active homoallylamines.
ChemInform Abstract: Catalyst‐Free, Direct, High Regio‐, and Chemoselective Conversion of Epoxides to 2‐Thiocyanatoalkyl Alkanoates under Neutral and Solvent‐Free Conditions.
Authors: Aghapour Ghasem , Hatefipour Razieh 
Published: December 2010

AbstractA variety of epoxides is converted to their corresponding thiocyanatoalkyl alkanoates in the presence of tetrabutylammonium rhodanide and aliphatic or aromatic carboxylic anhydrides.
ChemInform Abstract: Stereoselective Synthesis of Functionalized Cyclopropanes from Nitroalkenes via an Organocatalyzed Michael‐Initiated Ring‐Closure Approach.
Authors: Russo Alessio , Lattanzi Alessandra 
Published: December 2010

AbstractA convenient protocol to nitrocyclopropanes is developed by using commercially available α,α‐(2‐naphthyl)prolinol and bromomalonate with easily accessible trans‐nitrostyrenes.
ChemInform Abstract: Studies on the Asymmetric, Phosphine‐Promoted [3 + 2] Annulations of Allenic Esters with 2‐Aryl‐1,1‐dicyanoalkenes.
Authors: Schuler Marie , Voituriez Arnaud , Marinetti Angela 
Published: December 2010

AbstractThe phosphine (S)‐(BIP) promotes the cycloaddition to produce a number of cyclopentenes of type (III) in good to high yield and enantioselectivity.
ChemInform Abstract: Electronic and Steric Control of Regioselectivities in Rh(I)‐Catalyzed [5 + 2] Cycloadditions: Experiment and Theory.
Authors: Liu Peng , Sirois Lauren E. , Cheong Paul Ha‐Yeon , Yu Zhi‐Xiang , Hartung Ingo V. , Rieck Heiko , Wender Paul A. , Houk K. N. 
Published: December 2010

AbstractBulky and electron‐rich alkyne substituents are leading to preferential formation of (2,5)‐ [cf.
ChemInform Abstract: Palladium‐Catalyzed Asymmetric Umpolung Allylation of Imines with Allylic Alcohols.
Authors: Qiao Xiang‐Chen , Zhu Shou‐Fei , Chen Wang‐Qiao , Zhou Qi‐Lin 
Published: December 2010

AbstractThe direct use of highly stable and easily available allylic alcohols instead of allylic metal reagents facilitates the preparation of chiral homoallylic amines.
ChemInform Abstract: New Synthetic Route for Selectively Substituted 1,n‐Diamines. Synthesis of N‐Aryl Tetra‐ and Pentamethylenediamines.
Authors: Ramirez Maria A. , Corona Maria V. , Blanco Maria M. , Perillo Isabel A. , Porcal Williams , Salerno Alejandra 
Published: December 2010

AbstractVarious title compounds of biological interest are available via the hydrolysis of N‐acylalkylenediamine intermediates.
ChemInform Abstract: Axially Chiral Dicarboxylic Acid‐Catalyzed Asymmetric Imino Aza‐Enamine Reaction/Oxidation as a Strecker Reaction Surrogate.
Authors: Hashimoto Takuya , Kimura Hidenori , Maruoka Keiji 
Published: December 2010

AbstractThe described method provides a practical tool for the preparation of the Strecker‐type products without resorting to the use of highly toxic cyanide sources.
ChemInform Abstract: A Novel Application of (Diacetoxyiodo)benzene for Carbon—Carbon Cleavage of Aryl Diamines and Synthesis of Quinones.
Authors: Telvekar Vikas N. , Bachhav Harshal M. 
Published: December 2010

AbstractTreatment of various 1,2‐diamino aryl compounds under mild conditions A) results in C—C bond cleavage to afford dinitriles.
ChemInform Abstract: Straightforward Conversion of Arene Carboxylic Acids into Aryl Nitriles by Palladium‐Catalyzed Decarboxylative Cyanation Reaction.
Authors: Ouchaou Kahina , Georgin Dominique , Taran Frederic 
Published: December 2010

Abstractortho‐Substituted electron‐rich arene carboxylic acids react with cyanohydrin (II) to give nitriles with a broad spectrum concerning the product yield.