bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of donor site morbidity for free radial forearm osteocutaneous flaps
Authors: Sinclair Catherine F. , Gleysteen John P. , Zimmermann Terence M. , Wax Mark K. , Givi Babak , Schneider Daniel , Rosenthal Eben L. 
Published: April 2012

AbstractPurpose: Assessment of donor site morbidity and recipient site complications following free radial forearm osteocutaneous flap (FRFOCF) harvest and evaluation of patient perceived upper limb disability for free radial forearm osteocutaneous versus fasciocutaneous flaps (FRFF). Methods: First a case series was undertaken of 218 patients who underwent an FRFOCF at two tertiary referral centers between February 1998 and November 2010. Outcomes included forearm donor site morbidity and recipient site complications. Second, the disability of the arm, shoulder, and hand (DASH) questionnaire assessing patient perceived arm disability was administered by phone to 60 consecutive patients who underwent an FRFOCF or FRFF. Results: Mean patient age was 63 years with male predominance (62.8%). Median bone length harvested was 8 cm (range, 3–12 cm) with prophylactic plating of the radius following harvest. Donor site morbidity included fracture (1 patient, 0.5%) and sensory neuropathy (5 patients, 2.3%). Mean DASH scores were comparative between groups and to established normative values. Mandibular malunion rate was 3.2% and hardware extrusion at the recipient site occurred in 15.6%. Conclusion: Reluctance to perform FRFOCF by surgeons usually centers on concerns regarding potential donor site morbidity and adequacy of available bone stock; however, we identified minimal objective or patient perceived donor site morbidity or recipient site complications following harvest of FRFOCFs. Mild wrist weakness and stiffness are common but do not impede ability to perform activities of daily living. Data from this and other reports suggest this flap is particularly useful for midfacial and short segment mandibular reconstruction. © 2012 Wiley Periodicals, Inc. Microsurgery, 2012.
Vertical rectus abdominis musculocutaneous flow‐through flap to a free fibula flap for total sacrectomy reconstruction
Authors: Garvey Patrick B. , Clemens Mark W. , Rhines Laurence D. , Sacks Justin M. 
Published: April 2012

AbstractPurpose:The purpose of this report of a small series was to describe the technique of total sacrectomy reconstruction using a pedicled vertical rectus abdominis musculocutaneous (VRAM) flow‐through flap anastomosed to a free fibula flap.Methods:We reviewed all consecutive total sacrectomy reconstructions performed from 2009 to 2011. Surgical technique and patient outcomes were assessed.Results:Total sacrectomy reconstructions included three two‐stage and three‐stage VRAM flow‐through flap to free fibula flap patients all of which ambulated by discharge. Flap survival was 100%. Pelvic ring defects were reconstructed with A‐frame fibula flap struts anastomosed to the distal epigastric vessels of pedicled trans‐pelvic VRAM flaps. Complications such as wound healing, infection or hardware failure were not observed. Bony union occurred at an average 2.7 ± 0.6 months.Conclusions:Total sacrectomy reconstruction using a VRAM flow‐through flap anastomosed to a two‐strut free fibular flap allows initial assessment of the recipient vessels during the first and ensuing operative stages, satisfies the bone and soft tissue requirements of the defect, and provides a durable, functionally optimized reconstruction. © 2012 Wiley Periodicals, Inc. Microsurgery, 2013.
Urodynamic features and artefacts
Authors: Hogan Stephen , Gammie Andrew , Abrams Paul 
Published: April 2012

AbstractAimsThe aims of this study are to compile a list of common features and artefacts found in urodynamics, to produce definitions of these features, and describe any necessary remedial action. An image and word description for each event has been included as well as statistics providing information on the prevalence and frequency of each event.MethodsIn order to identify the most common features and artefacts 200 consecutive urodynamic traces were reviewed. A random 10% sample was cross‐checked to ensure event classification accuracy. To extract significant pressure peaks from the data, an algorithm was written capable of detecting initial resting pressure and updating it as necessary. Significant pressure peaks were defined as those that differed from resting pressure values by 10 cmH2O or more. When describing the events, standard sources were consulted for published definitions. The images selected for each event are typical examples but do not represent the variation that can occur between examples. The patients whose files were used in this study suffered from a variety of lower urinary tract disorders so that it is likely that they cover all common or important urodynamic pressure events.ResultsIn total 10,355 pressure events were identified and classified into 19 different categories. For each category, a description, example image and remedial action are included. Where published definitions do not exist, new ones are proposed.ConclusionsAll common or important urodynamic pressure events have been included in this study and descriptions of the events have been included in one article for the first time. Neurourol. Urodynam. 31:1104–1117, 2012. © 2012 Wiley Periodicals, Inc.
Prenatal BACs‐on‐BeadsTM: the prospective experience of five prenatal diagnosis laboratories
Authors: Vialard François , Simoni Giuseppe , Gomes Denise Molina , Abourra Azzedine , Toffol Simona De , Bru Fabrice , Romero Maria Carmen Martinez , Nitsch Lucio , Bouhanna Philippe , Marcato Livia , Popowski Thomas , Grimi Beatrice , Martínez‐Conejero Jose Antonio , Benzacken B. , Genesio Rita , Grati Francesca R. 
Published: April 2012

ABSTRACTObjectiveWe previously reported on the validation of Prenatal BACs‐on‐BeadsTM on retrospectively selected and prospective prenatal samples. This bead‐based multiplex assay detects chromosome 13, 18, 21 and X/Y aneuploidies and the nine most frequent microdeletion syndromes. We demonstrated that Prenatal BACs‐on‐BeadsTM is a new‐generation, prenatal screening tool. Here, we describe the experience of five European prenatal diagnosis laboratories concerning the ongoing use of Prenatal BACs‐on‐BeadsTM.MethodsSome 1653 samples were analyzed. All results were confirmed by conventional karyotyping or another appropriate technique. All indications for invasive prenatal diagnosis were included. Amniotic fluid and chorionic villus samples were analyzed in equivalent proportions.ResultsThe failure rate was 3.3% and the overall abnormality detection rate was ~1/10. Eighty‐five percent of the detected abnormalities were common aneuploidies. Eleven microdeletions and duplications were identified, thus giving an overall yield for microdeletion and microduplication detection of 1/145. Compared with QF‐PCR, Prenatal BACs‐on‐BeadsTM provides an additional detection rate of ~1/250 for low‐risk pregnancies. The false positive and negative rates were both <1%.ConclusionWhen associated with conventional karyotyping, the Prenatal BACs‐on‐BeadsTM assay combines a short turnaround time (typical of rapid aneuploidy detection tests) with valuable detection of the most frequent microdeletion syndromes that cannot be detected in cytogenetic analyses. © 2012 John Wiley & Sons, Ltd.
Same‐day prenatal diagnosis of common chromosomal aneuploidies using microfluidics‐fluorescence in situ hybridization
Authors: Ho Sherry S. Y. , Chua Cuiwen , Gole Leena , Biswas Arijit , Koay Evelyn , Choolani Mahesh 
Published: April 2012

ABSTRACTRapid molecular prenatal diagnostic methods, such as fluorescence in situ hybridization (FISH), quantitative fluorescence‐PCR, and multiplex ligation‐dependent probe amplification, can detect common fetal aneuploidies within 24 to 48 h. However, specific diagnosis or aneuploidy exclusion should be ideally available within the same day as fetal sampling to alleviate parental anxiety. Microfluidic technologies integrate different steps into a microchip, saving time and costs. We have developed a cost‐effective, same‐day prenatal diagnostic FISH assay using microfluidics. Amniotic fluids (1–4 mL from 40 pregnant women at 15–22 weeks of gestation) were fixed with Carnoy's before loading into the microchannels of a microfluidic FISH‐integrated nanostructured device. The glass slides were coated with nanostructured titanium dioxide to facilitate cell adhesion. Pretreatment and hybridization were performed within the microchannels. Fifty nuclei were counted by two independent analysts, and all results were validated with their respective karyotypes. Of the 40 samples, we found three cases of fetal aneuploidies (trisomies 13, 18, and 21), whereas the remaining 37 cases were normal. Results were concordant with their karyotypes and ready to be released within 3 h of sample receipt. Microfluidic FISH, using 20‐fold less than the recommended amount of probe, is a cost‐effective method to diagnose common fetal aneuploidies within the same day of fetal sampling. Copyright © 2011 John Wiley & Sons, Ltd.
Referral patterns for microarray testing in prenatal diagnosis
Authors: Shaffer Lisa G. , Dabell Mindy Preston , Rosenfeld Jill A. , Neill Nicholas J. , Ballif Blake C. , Coppinger Justine , Diwan Noa Rinzler , Chong Karen , Shohat Mordechai , Chitayat David 
Published: April 2012

ABSTRACTObjectiveTo understand the prenatal referral patterns from the United States, Canada, and Israel for two whole‐genome microarray platforms, each with a different resolution.MethodPhysicians selected one of the two array designs to be performed on 1483 prenatal specimens for a 1‐year period. We retrospectively examined detection rates, indications for study, and physician array selection.ResultsThe lower resolution array (55 K) showed an ~32% decrease in the detection of results of unclear clinical significance while retaining the ability to detect all but one significant abnormality identified by the higher resolution array (135 K). A majority of samples were referred for abnormal ultrasound findings. Whereas the United States and Canada utilized the higher resolution array more often for this indication, Israel preferred the 55 K array. Referral patterns for parental anxiety were similar for the United States and Israel, with most cases being tested on the 55 K array. Few cases were referred for advanced maternal age or family history of a genetic condition from either Canada or Israel.ConclusionReferral patterns varied between the countries and between indications for study. Understanding these differences will provide laboratories the critical information needed to develop array designs to meet the medical needs and patient desires for prenatal testing. © 2012 John Wiley & Sons, Ltd.
Diffusion and solubility of commercial poly(methyl methacrylate) denture base material modified with dimethyl itaconate and di‐n‐butyl itaconate during water absorption/desorption cycles
Authors: Spasojević Pavle , Stamenković Dragoslav , Pjanović Rada , Bošković‐Vragolović Nevenka , Dolić Jelena , Grujić Svetlana , Veličković Sava 
Published: April 2012

AbstractThis study evaluated the water absorption, solubility, kinetics of water diffusion and residual monomer content of commercial poly(methyl methacrylate) (PMMA) denture base material modified with dimethyl itaconate (DMI) and di‐n‐butyl itaconate (DBI). Water absorption and solubility were measured gravimetrically while the residual monomer content was analysed using high‐performance liquid chromatography with ultraviolet detection. It was found that the addition of di‐n‐alkyl itaconates significantly decreases the residual methyl methacrylate (MMA) content in the polymerized material. Maximum uptake (M∞) and loss (M′∞), and diffusion coefficients for absorption (Da) and desorption (Dd) of water through all materials were established. M∞ shows a linear decrease with increasing amount of itaconate in the system while Da shows a linear increase with increasing amount of itaconate, both of these effects being more pronounced when DBI is present compared to DMI. M∞ is a linear function of the value of Hoy's solubility parameter. The reduction in residual MMA promoted by addition of a small amount of di‐n‐alkyl itaconates can improve the applicative properties and biocompatibility of the PMMA denture base material. Also, it is shown that modification of the denture base material with di‐n‐alkyl itaconates can enable precise control of water absorption in the system. Copyright © 2012 Society of Chemical Industry
SET‐LRP goes “green”: Various hemicellulose initiating systems under non‐inert conditions
Authors: Edlund Ulrica , Albertsson Ann‐Christine 
Published: April 2012

AbstractRobust and versatile controlled radical polymerization, also in air, was achieved via single electron transfer living radical polymerization (SET‐LRP) initiated by purposely designed hemicellulose‐derived macroinitiators. The efficiency of the substitution reaction, converting the polysaccharides into bromo‐multifunctionalized initiators, as well as the rate of subsequent induced polymerizations of methyl acrylate were controlled by the hemicellulose repeating unit structure, branching pattern, and molecular weight. Macroinitiators with mannan‐based backbones induce SET‐LRP with somewhat higher apparent rate constants than xylan‐derived analogues, increasing by a factor two to three when raising the reaction temperature from 25 to 40 °C. The presence of lignin in a nonpurified xylan fraction did not impair its viability as a macroinitiator. Hemicellulose‐initiated SET‐LRP was feasible in air, proceeding with comparable or somewhat higher apparent rate constants than when conducted under deoxygenated conditions. © 2012 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2012
Glycopolymers obtained by chemical modification of well‐defined block copolymers
Authors: Muñoz‐Bonilla Alexandra , León Orietta , Cerrada Maria L. , Rodríguez‐Hernández Juan , Sánchez‐Chaves Manuel , Fernández‐García Marta 
Published: April 2012

AbstractWell‐defined di and triblock copolymers based on 2‐hydroxyethyl methacrylate, HEMA, and n‐butyl acrylate, BA, have been synthesized by atom transfer radical polymerization, ATRP. Two copolymers have been selected to prepare glycopolymers containing D‐(+)‐glucosamine or N‐(4‐aminobutyl)‐D‐gluconamide, NABG, by chemical modification of HEMA units. The chemical modification occurs by activation of the hydroxyl groups of the HEMA units with highly reactive p‐nitrophenyl carbonate groups. The block copolymers structure and self‐assembly have been comprehensively studied in solid state and in solution by differential scanning calorimetry, X‐ray diffraction, atomic force microscopy and dynamic light scattering. The resulting glycopolymers obtained upon chemical modification turned out to be water soluble. Therefore, their carbohydrate‐lectin interactions of have been analyzed by turbidimetry measurements. © 2012 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2012
A vinylene‐linked benzo[1,2‐b:4,5‐b']dithiophene‐2,1,3‐benzothiadiazole low‐bandgap polymer
Authors: Abbotto Alessandro , Seri Mirko , Dangate Milind S. , De Angelis Filippo , Manfredi Norberto , Mosconi Edoardo , Bolognesi Margherita , Ruffo Riccardo , Salamone Matteo M. , Muccini Michele 
Published: April 2012

AbstractA new heteroarylene‐vinylene donor–acceptor polymer P(BDT‐V‐BTD) with reduced bandgap has been synthesized and its photophysical, electronic and photovoltaic properties investigated both experimentally and theoretically. The structure of the polymer comprises an unprecedented combination of a strong donor (4,8‐dialkoxy‐benzo[1,2‐b:4,5‐b']dithiophene, BDT), a strong acceptor (2,1,3‐benzothiadiazole, BTD) and a vinylene spacer. The new polymer was obtained by a metal‐catalyzed cross‐coupling Stille reaction and fully characterized by NMR, UV–vis absorption, GPC, TGA, DSC and electrochemistry. Detailed ab initio computations with solvation effects have been performed for the monomer and model oligomers. The electrochemical investigation has ascertained the ambipolar character of the polymer and energetic values of HOMO, LUMO and bandgap matching materials‐design rules for optimized organic photovoltaic devices. The HOMO and LUMO energies are consistently lower than those of previous heteroarylene‐vinylene polymer while the introduction of the vinylene spacer afforded lower bandgaps compared to the analogous system P(BDT‐BTD) with no spacer between the aromatic rings. These superior properties should allow for enhanced photovoltages and photocurrents in photovoltaic devices in combination with PCBM. Preliminary photovoltaic investigation afforded relatively modest power conversion efficiencies of 0.74% (AM 1.5G, 100 mW/cm2), albeit higher than that of previous heteroarylene‐vinylene polymers and comparable to that of P(BDT‐BTD). © 2012 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2012
Severe Mycoplasma pneumoniae infection requiring extracorporeal membrane oxygenation with concomitant ischemic stroke in a child
Authors: Garcia Alejandro V. , Fingeret Abbey L. , Thirumoorthi Arul S. , Kadenhe‐Chiweshe Angela , Kandel Jessica J. 
Published: April 2012

AbstractMycoplasma pneumoniae is one of the most common agents causing respiratory disease in children. The most common extra‐pulmonary manifestations of M. pneumoniae include central nervous system involvement, with stroke being an uncommon but devastating consequence. We present a 13‐year‐old girl with severe respiratory disease requiring extracorporeal membrane oxygenation, who developed ischemic stroke associated with clinical and serologic evidence of M. pneumoniae. A case of M. pneumoniae causing this degree of respiratory failure associated with stroke has not been previously reported. Prompt recognition of severe mycoplasmal infection may allow for earlier treatment and concomitant evaluation of neurologic injury. Pediatr Pulmonol. 2013; 48:98–101. © 2012 Wiley Periodicals, Inc.
Integrating asthma education and smoking cessation for parents: Financial return on investment
Authors: McQuaid Elizabeth L. , Garro Aris , Seifer Ronald , Hammond S. Katharine , Borrelli Belinda 
Published: April 2012

AbstractBackgroundCaregivers who smoke and have children with asthma are an important group for intervention. Home‐based interventions successfully reduce asthma morbidity, yet are costly. This study evaluated the financial return on investment (ROI) of the Parents of Asthmatics Quit Smoking (PAQS) program, a combined asthma education and smoking cessation intervention.MethodsParticipants included caregivers (n = 224) that smoked, had a child with asthma, and were enrolled in a Medicaid managed care plan. Participants received nurse‐delivered asthma education and smoking counseling in three home visits. Program implementation costs were estimated, and healthcare expenses were obtained from insurance claims data 12 months pre‐ and 12 months post intervention. ROI was calculated for all participants, children <6 years, children 6–18 years, and children with moderate/severe persistent asthma.ResultsTotal program implementation cost was $34,481. After intervention, there was increased mean annual refills of beta‐agonist (0.51 pre, 1.64 post; P < 0.001), and controller medications (0.65 pre, 2.44 post; P < 0.001). Reductions were found in mean annual emergency department visits (0.33 pre, 0.14 post; P < 0.001), hospitalizations (0.23 pre, 0.08 post; P < 0.001), and outpatient visits (2.33 pre, 1.45 post, P < 0.001). The program had negative ROI (−21.8%) for the entire sample. The ROI was positive (+106.9) for children <6 years, negative (−150.3) for children 6–18, and negligible for moderate/severe persistent asthma (+6.9%).ConclusionPAQS was associated with increased medication use and decreased healthcare utilization. While the overall ROI for PAQS was negative, PAQS had a positive ROI for caregivers of young children with asthma. Pediatr Pulmonol. 2012. 47:950–955. © 2012 Wiley Periodicals, Inc.
Sublimation Rate of Energetic Materials in Air: RDX and PETN
Authors: Gershanik Arcady P. , Zeiri Yehuda 
Published: April 2012

AbstractThis paper reports measurements of RDX and PETN sublimation rate in air. For both explosives the initial samples were prepared by precipitation from solution. For RDX a continuous thin film is obtained, while PETN samples were constituted of small, well separated, single crystals. The sublimation process was monitored using a quartz crystal microbalance (QCM). It is demonstrated that a quantitative description of the sublimation rates can be obtained using molecular diffusion equations in the surrounding air. A quantitative description is limited by the large discrepancy of the reported vapor pressure of these explosives in the literature. An additional obstacle in the accuracy of these predictions is the limited data related to vapor‐in‐air diffusion coefficients. The results reported herein allow us to determine the most relevant vapor pressure source among those reported in the literature and the most probable diffusion coefficient values.
Structural basis of interleukin‐5 dimer recognition by its α receptor
Authors: Kusano Seisuke , Kukimoto‐Niino Mutsuko , Hino Nobumasa , Ohsawa Noboru , Ikutani Masashi , Takaki Satoshi , Sakamoto Kensaku , Hara‐Yokoyama Miki , Shirouzu Mikako , Takatsu Kiyoshi , Yokoyama Shigeyuki 
Published: April 2012

AbstractInterleukin‐5 (IL‐5), a major hematopoietin, stimulates eosinophil proliferation, migration, and activation, which have been implicated in the pathogenesis of allergic inflammatory diseases, such as asthma. The specific IL‐5 receptor (IL‐5R) consists of the IL‐5 receptor α subunit (IL‐5RA) and the common receptor β subunit (βc). IL‐5 binding to IL‐5R on target cells induces rapid tyrosine phosphorylation and activation of various cellular proteins, including JAK1/JAK2 and STAT1/STAT5. Here, we report the crystal structure of dimeric IL‐5 in complex with the IL‐5RA extracellular domains. The structure revealed that IL‐5RA sandwiches the IL‐5 homodimer by three tandem domains, arranged in a “wrench‐like” architecture. This association mode was confirmed for human cells expressing IL‐5 and the full‐length IL‐5RA by applying expanded genetic code technology: protein photo‐cross‐linking experiments revealed that the two proteins interact with each other in vivo in the same manner as that in the crystal structure. Furthermore, a comparison with the previously reported, partial GM‐CSF•GM‐CSFRA•βc structure enabled us to propose complete structural models for the IL‐5 and GM‐CSF receptor complexes, and to identify the residues conferring the cytokine‐specificities of IL‐5RA and GM‐CSFRA.