bluuebunny/crossref_metadata_2025_split huggingface dataset:
Synergistic activation by p38MAPK and glucocorticoid signaling mediates induction of M2‐like tumor‐associated macrophages expressing the novel CD20 homolog MS4A8A
Authors: Schmieder Astrid , Schledzewski Kai , Michel Julia , Tuckermann Jan P. , Tome Lydia , Sticht Carsten , Gkaniatsou Cleopatra , Nicolay Jan P. , Demory Alexandra , Faulhaber Jörg , Kzhyshkowska Julia , Géraud Cyrill , Goerdt Sergij 
Published: September 2010

AbstractTumor‐associated macrophages (TAMs) represent alternatively activated (M2) macrophages that support tumor growth. Previously, we have described a special LYVE‐1+ M2 TAM subset in vitro and in vivo; gene profiling of this TAM subset identified MS4A8A as a novel TAM molecule expressed in vivo by TAM in mammary carcinoma and malignant melanoma. In vitro, Ms4a8a mRNA and MS4A8A protein expression was strongly induced in bone marrow‐derived macrophages (BMDMs) by combining M2 mediators (IL‐4, glucocorticoids) and tumor‐conditioned media (TCM). Admixture of MS4A8A+ TCM/IL‐4/GC‐treated BMDM significantly enhanced the tumor growth rate of subcutaneously transplanted TS/A mammary carcinomas. Upon forced overexpression of MS4A8A, Raw 264.7 macrophage‐like cells displayed a special gene signature. Admixture of these MS4A8A+ Raw 264.7 cells also significantly enhanced the tumor growth rate of subcutaneously transplanted mammary carcinomas. To identify the signaling pathways involved in synergistic induction of MS4A8A, the major signaling cascades with known functions in TAM were analyzed. Although inhibitors of NF‐κB activation and of the MAPK JNK and ERK did not show relevant effects, the p38α/β MAPK inhibitor SB203580 strongly and highly significantly (p > 0.001) inhibited MS4A8A expression on mRNA and protein level. In addition, MS4A8A expression was restricted in M2 BMDM from mice with defective GC receptor (GR) dimerization indicating that classical GR gene regulation is mandatory for MS4A8A induction. In conclusion, expression of MS4A8A within the complex signal integration during macrophage immune responses may act to fine tune gene regulation. Furthermore, MS4A8A+ TAM may serve as a novel cellular target for selective cancer therapy.
Anti‐breast cancer potential of SS5020, a novel benzopyran antiestrogen
Authors: Suzuki Naomi , Liu Xiaoping , Laxmi Y. R. Santosh , Okamoto Kanako , Kim Hyo Jeong , Zhang Guangxiang , Chen John J. , Okamoto Yoshinori , Shibutani Shinya 
Published: September 2010

AbstractTreatment with tamoxifen (TAM) increases the risk of developing endometrial cancer in women. The carcinogenic effect is thought to involve initiation and/or promotion resulting from DNA damage induced by TAM as well as its estrogenic action. To minimize this serious side‐effect while increasing the anti‐breast cancer potential, a new benzopyran antiestrogen, 2E‐3‐{4‐[(7‐hydroxy‐2‐oxo‐3‐phenyl‐2H‐chromen‐4‐yl)‐methyl]‐phenyl}‐acrylic acid (SS5020), was synthesized. Unlike TAM, SS5020 exhibits no genotoxic activity to damage DNA. Furthermore, SS5020 does not present significant uterotrophic potential in rats; in contrast, the structurally related compounds, TAM, toremifene, raloxifene (RAL) and SP500263 all have uterotrophic activity. At the human equivalent molar dose of TAM (0.33 or 1.0 mg/kg), SS5020 had much stronger antitumor potential than those same antiestrogens against 7,12‐dimethylbenz(a)anthracene‐induced mammary carcinoma in rats. The growth of human MCF‐7 breast cancer xenograft implanted into athymic nude mice was also effectively suppressed by SS5020. SS5020, lacking genotoxic and estrogenic actions, could be a safer and stronger antiestrogen alternative to TAM and RAL for breast cancer therapy and prevention.
The novel histone deacetylase inhibitor, AR‐42, inhibits gp130/Stat3 pathway and induces apoptosis and cell cycle arrest in multiple myeloma cells
Authors: Zhang Shuhong , Suvannasankha Attaya , Crean Colin D. , White Valerie L. , Chen Ching‐Shih , Farag Sherif S. 
Published: September 2010

AbstractMultiple myeloma (MM) remains incurable with current therapy, indicating the need for continued development of novel therapeutic agents. We evaluated the activity of a novel phenylbutyrate‐derived histone deacetylase inhibitor, AR‐42, in primary human myeloma cells and cell lines. AR‐42 was cytotoxic to MM cells at a mean LC50 of 0.18 ± 0.06 μmol/l at 48 hr and induced apoptosis with cleavage of caspases 8, 9 and 3, with cell death largely prevented by caspase inhibition. AR‐42 downregulated the expression of gp130 and inhibited activation of STAT3, with minimal effects on the PI3K/Akt and MAPK pathways, indicating a predominant effect on the gp130/STAT‐3 pathway. AR‐42 also inhibited interleukin (IL)‐6‐induced STAT3 activation, which could not be overcome by exogenous IL‐6. AR‐42 also downregulated the expression of STAT3‐regulated targets, including Bcl‐xL and cyclin D1. Overexpression of Bcl‐xL by a lentivirus construct partly protected against cell death induced by AR‐42. The cyclin dependent kinase inhibitors, p16 and p21, were also significantly induced by AR‐42, which together with a decrease in cyclin D1, resulted in G1 and G2 cell cycle arrest. In conclusion, AR‐42 has potent cytotoxicity against MM cells mainly through gp130/STAT‐3 pathway. The results provide rationale for clinical investigation of AR‐42 in MM.
Podocyte‐specific VEGF down‐regulation and pathophysiological development
Authors: Zhang Shuzhi , Ji Yan , Liu Xiao , Lu Xiaodan , Su Weiheng , Zhang Di , Hao Feng , Yi Fei , Guo Lei , Li Xiaomeng , Zheng Yaowu 
Published: September 2010

AbstractIt is well‐known that vascular endothelial growth factor (VEGF) plays a key role in development and pathology, but its function in normal adult tissues is rarely understood. Increased use of anti‐angiogenic therapies targeting VEGF in human pathologies have shown more and more adverse effects. In this report, a conditional expression model (Tet‐On system) was used to down‐regulate podocyte VEGF in adult mice, which resulted in many kidney problems, characterized by glomerular morphological changes, proteinuria, reduced water consumption and urination, increased urine electro‐conductivity, as well as high susceptibility to BSA stress. Our findings indicated that podocyte‐specific VEGF down‐regulation resulted in poor kidney performance and led mice to be more susceptible to further kidney damages. © 2010 IUBMB IUBMB Life, 62(9): 677–683, 2010
An in vitro study of plasticized poly(lactic‐co‐glycolic acid) films as possible guided tissue regeneration membranes: Material properties and drug release kinetics
Authors: Owen Gethin Rh. , Jackson John K. , Chehroudi Babak , Brunette Donald M. , Burt Helen M. 
Published: September 2010

AbstractGuided tissue regeneration (GTR), in periodontal therapy, involves the placement of a barrier membrane, to ensure the detached root surface becomes repopulated with periodontal ligament cells capable of regenerating this attachment. GTR procedures exhibit large variability in surgical outcome as a consequence of poor membrane performance. The objective of this study was to evaluate the suitability of plasticized poly(lactic‐co‐glycolic acid) (PLGA) as a material for GTR membranes. The material was also investigated as a localized controlled release system for the antibiotic, anti‐inflammatory agent tetracycline. Films made from PLGA (85:15), plasticized with either 10% w/v methoxypoly(ethyleneglycol) (MePEG) or a diblock copolymer [poly(D,L‐lactic acid)‐block‐methoxypoly(ethyleneglycol)] were loaded with tetracycline base (or hydrochloride salt) and cast by solvent evaporation. Drug release was measured using high performance liquid chromatography (HPLC). The time‐course of elasticity changes and swelling were determined using a stress–strain apparatus or gravimetric/dimensional determinations, respectively. Cells extracted from periodontal ligament cell explants were used to evaluate the effect of material and drug loading on cell morphology. Tetracycline·HCl released more rapidly than tetracycline from PLGA films. The addition of either MePEG or diblock caused a concentration dependent increase in release rates for both drugs. Release profiles ranged from a small initial burst phase followed by slow sustained release to almost full drug release after 1 day. After incubation in PBS, the films stiffened and swelled within 30 min. Periodontal ligament cell morphology was not affected by the inclusion of tetracycline. Plasticized PLGA films displayed desired features for possible use as GTR membranes. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Genipin‐crosslinked silk fibroin/hydroxybutyl chitosan nanofibrous scaffolds for tissue‐engineering application
Authors: Zhang Kuihua , Qian Yongfang , Wang Hongsheng , Fan Linpeng , Huang Chen , Yin Anlin , Mo Xiumei 
Published: September 2010

AbstractTo improve water‐resistant ability and mechanical properties of silk fibroin (SF)/hydroxybutyl chitosan (HBC) nanofibrous scaffolds for tissue‐engineering applications, genipin, glutaraldehyde (GTA), and ethanol were used to crosslink electrospun nanofibers, respectively. The mechanical properties of nanofibrous scaffolds were obviously improved after 24 h of crosslinking with genipin and were superior to those crosslinked with GTA and ethanol for 24 h. SEM indicated that crosslinked nanofibers with genipin and GTA vapor had good water‐resistant ability. Characterization of the microstructure (porosity and pore structure) demonstrated crosslinked nanofibrous scaffolds with genipin and GTA vapor had lager porosities and mean diameters than those with ethanol. Characterization of FTIR‐ATR and 13C NMR clarified both genipin and GTA acted as crosslinking agents for SF and HBC. Furthermore, genipin could induce SF conformation from random coil or α‐helix to β‐sheet. Although GTA could also successfully crosslink SF/HBC nanofibrous scaffolds, in long run, genipin maybe a better method due to lower cytotoxicity than GTA. Cell viability studies and wound‐healing test in rats clarified that the genipin‐crosslinked SF/HBC nanofibrous scaffolds had a good biocompatibility both in vitro and in vivo. These results suggested that genipin‐crosslinked SF/HBC nanofibrous scaffolds might be potential candidates for wound dressing and tissue‐engineering scaffolds. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010.
Evidence‐based selection criteria to protect blood donors
Authors: Eder Anne 
Published: September 2010

AbstractA safe and adequate blood supply depends on healthy, volunteer blood donors. Blood centers have instituted various screening procedures in an effort to determine that donors are free of diseases that can be transmitted to patients by blood transfusion and are able to tolerate the collection procedure without experiencing significant complications. This review focuses on selection criteria intended to minimize the risk to the blood donor. Defining a rational, evidence‐based approach to donor selection is crucial not only to take reasonable precautions to protect the donors' health but also to eliminate practices that lead to the unnecessary deferral of large numbers of people without improving the safety of the donation process. Donor selection criteria, including predonation pulse and blood pressure, donor weight and total blood volume, minimum hemoglobin and donation interval, are considered against the available evidence that support or suggest the need to modify the current approach to protect blood donor health. J. Clin. Apheresis, 2010. © 2010 Wiley‐Liss, Inc.
Teaching and learning apheresis medicine: The Bermuda Triangle in Education
Authors: Crookston Kendall P. , Richter Deana M. 
Published: September 2010

AbstractApheresis Medicine has evolved markedly due to an explosion of knowledge and technology, whereas the time available for training has shrunk as curricula have become increasingly overloaded. Apheresis teaching has inherited a strong clinical context where real patient problems are used in a hands‐on environment. To optimize instruction, those involved in the education of apheresis professionals need to have (1) knowledge of how clinical laboratory medicine education has developed as a field, (2) an understanding of what is known from theory and research about how people learn, and (3) the skills to design teaching/learning activities in ways consistent with literature‐based principles of adult education. These developments in education provide a context for curriculum projects currently underway by the American Society for Apheresis. Teachers must determine which competencies are central to the essence of a trained professional. Specific, robust, learning objectives targeted toward the development of higher levels of thinking, professional attitudes, and requisite skills are formulated to guide the learner toward mastering those competencies. Curriculum is developed for each objective, consisting of content and the best teaching/learning methods to help learners attain the objective. Appropriate assessment strategies are identified to determine whether the objective is being achieved. The integration of objectives, curriculum, and assessment creates The Bermuda Triangle of Education (Richter, The Circle of Learning and Bermuda Triangle in Education, University of New Mexico School of Medicine, 2004). When educators do not effectively navigate The Bermuda Triangle of Education, learning may disappear into the murky depths of confusion and apathy. When successfully navigated, the result will be a significant learning experience that leads to transformation through education. J. Clin. Apheresis, 2010. © 2010 Wiley‐Liss, Inc.
Effects of protein A immunoadsorption in patients with chronic dilated cardiomyopathy
Authors: Doesch Andreas O. , Mueller Susanne , Konstandin Mathias , Celik Sultan , Kristen Arnt , Frankenstein Lutz , Goeser Stefan , Kaya Ziya , Zugck Christian , Dengler Thomas J. , Katus Hugo A. 
Published: September 2010

AbstractObjectives:The objective of this study was to investigate functional effects of immunoadsorption (IA) in patients with chronic nonfamilial dilated cardiomyopathy (DCM) regarding clinical and humoral markers of heart failure.Background:IA has been shown to induce early hemodynamic improvement in patients with nonfamilial dilated cardiomyopathy (DCM).Methods:We performed IA using protein A agarose columns on five consecutive days in 51 patients with chronic DCM, congestive heart failure of NYHA class ≥ II, left ventricular ejection fraction ≤50%, and mean time since initial diagnosis of 5.0 ± 5.8 years.Results:Immediately after IA, immunoglobulin G (IgG) decreased by 89.4% and IgG3 by 66.7% (both P < 0.0001). Median NT‐pro BNP was reduced from 1230.0 ng L−1 at baseline to 829.0 ng L−1 after 6 months (P < 0.0001). Also mean left ventricular ejection fraction (LVEF) was significantly improved (26.3% ± 9.4% to 28.7% ± 11.4% after 6 months, P = 0.016) and LVEF improved ≥5% (absolute) in 21 of 51 (41.2%) patients. After 6 months, bicycle spiroergometry showed a significant increase in exercise capacity from 82.0 ± 30.8 Watts to 93.1 ± 34.3 Watts (P = 0.008) while VO2max rose from 15.0 ± 4.1 to 16.4 ± 4.8 mL min−1 kg−1 (P = 0.01).Conclusions:In this study, on heart failure patients with nonfamilial DCM, IA therapy significantly improved clinical and humoral markers of heart failure severity. These promising results may be due to the selected study population, with a shorter disease duration and the higher amount of IgG 3 reduction. Future blinded prospective multicenter studies are necessary to identify those patients that benefit most. J. Clin. Apheresis, 2010. © 2010 Wiley‐Liss, Inc.
Change in defense mechanisms and coping over the course of short‐term dynamic psychotherapy for adjustment disorder
Authors: Kramer Ueli , Despland Jean‐Nicolas , Michel Luc , Drapeau Martin , de Roten Yves 
Published: September 2010

AbstractShort‐term dynamic psychotherapy (STDP) has rarely been investigated with regard to its underlying mechanisms of change, even if psychoanalytic theory informs us about several potential putative mechanisms of change in patients. Change in overall defensive functioning is one. In this study, we explored the role of overall defensive functioning, by comparing it on the process level with the neighbouring concept of overall coping functioning. A total ofN=32 patients, mainly presenting adjustment disorder, were included in the study. The patients underwent STDP up to 40 sessions; three sessions per psychotherapy were transcribed and analyzed by using two observer‐rating scales: Defense Mechanism Rating Scales (Perry,1990) and Coping Action Patterns (Perry, Drapeau, Dunkley, & Blake,2005). Hierarchical linear modeling was applied to model the change over the course of therapy and relate it to outcome. Results suggest that STDP has an effect on the target variable of overall defensive functioning, which was absent for overall coping functioning. Links with outcome confirm the importance of the effect. These results are discussed from methodological and clinical viewpoints. © 2010 Wiley Periodicals, Inc. J Clin Psychol 66:1–10, 2010.
Hunter‐gatherer response to late Holocene climatic variability in northern and central Australia
Authors: Williams Alan N. , Ulm Sean , Goodwin Ian D. , Smith Mike 
Published: September 2010

AbstractSum probability analysis of 1275 radiometric ages from 608 archaeological sites across northern and central Australia demonstrates a changing archaeological signature that can be closely correlated with climate variability over the last 2 ka. Results reveal a marked increase in archaeological records across northern and central Australia over the last 2 ka, with notable declines in western and northern Australia between ca. AD 700 and 1000 and post‐AD 1500 – two periods broadly coeval with the Medieval Climatic Anomaly and the Little Ice Age as they have been documented in the Asia–Pacific region. Latitudinal and longitudinal analysis of the dataset suggests the increase in archaeological footprint was continent wide, while the declines were greatest from 9 to 20° S, 110 to 135° E and 143 to 150° E. The change in the archaeological data suggests that, combined with an increase in population over the late Holocene, a disruption or reorganisation of pre‐European resource systems occurred across Australia between ca. AD 700 and 1000 and post‐AD 1500. These archaeological responses can be broadly correlated with transitions of the El Niño–Southern Oscillation (ENSO) mean state on a multi‐decadal to centennial timescale. The latter involve a shift towards the La Niña‐like mean state with wetter conditions in the Australian region between AD 700 and 1150. A transition period in ENSO mean state occurred across Australia during AD 1150–1300, with persistent El Niño‐like and drier conditions to ca. AD 1500, and increasing ENSO variability post‐AD 1500 to the present. Copyright © 2010 John Wiley & Sons, Ltd.
A 70 ka record of explosive eruptions from the TALDICE ice core (Talos Dome, East Antarctic plateau)
Authors: Narcisi Biancamaria , Petit Jean Robert , Chappellaz Jérôme 
Published: September 2010

AbstractThe new Antarctic TALDICE ice core (72° 49′ S, 159° 11′ E, 1620 m depth), containing abundant primary tephras, provides the opportunity to elucidate the late Quaternary volcanic history of the south polar region, as well as to broaden the East Antarctic tephrostratigraphic framework. Here grain size and glass compositional data for representative tephra layers from the last 70 ka core section are used for source identification. Results point to origin of layers from centres of the Melbourne Volcanic Province (McMurdo Volcanic Group), located ∼250 km from the coring site. Occurrence of tephra layers within the ice core record suggests that explosive activity in the identified source was not constant over the considered period, with a minimum of activity between 20 and 35 ka, and increased activity back to 65 ka. In addition to palaeovolcanic implications, the TALDICE tephra layers offer prospects for firm correlations between diverse widely separated palaeoarchives and for accurate dating of the Antarctic climatic record. Copyright © 2010 John Wiley & Sons, Ltd.
Preparation and characterization of molecularly imprinted silica monolith for screening sulfamethazine
Authors: He Jin‐Xing , Fang Guo‐zhen , Yao Yu‐chao , Wang Shuo 
Published: September 2010

AbstractIn this work, a novel approach of preparing molecularly imprinted film‐derivatized silica monolith materials was developed by a two‐step procedure. The silica monolithic support was first prepared by the sol–gel method with tetramethoxysilane as the precursor. Subsequently, vinyl groups were introduced onto the surface of silica monolith by immobilization of γ‐methacryloxypropyltrimethoxysilane. The prepolymerization mixtures, consisting of methacrylic acid as a functional monomer, ethylene dimethacrylate as a crosslinker, sulfamethazine as a template molecule and an ionic liquid as porogen, were injected into the silica monolith immobilized vinyl groups to form the molecularly imprinted films on the surface of the vinyl functionalized silica monolith. The monolithic materials were characterized by SEM, Fourier transform IR and solid‐state reflection UV spectra. The resulted imprinted materials were evaluated under CEC and HPLC mode. The results indicated that there were enough recognition sites on the surface of the imprinted film‐derivatized monolithic materials for selectively recognizing sulfamethazine from the sulfonamide mixture. Ionic liquids, which was utilized as the porogens, could improve the flow‐through property and the imprinting effect of the molecularly imprinted film‐functionalized silica monolithic materials.
SPE isolation of low‐molecular oxygen compounds from essential oils
Authors: Dawidowicz Andrzej L. , Dybowski Michal P. 
Published: September 2010

AbstractAnalytical procedures of complex mixtures frequently involve their initial pre‐fractionation to make the analysis easier or possible. Recently, SPE is frequently applied for this purpose. This article discusses the possibility of essential oil's fractionation using SPE with octadecyl modified silica. The presented results show that developed SPE with C‐18 sorbent allows for easy and total isolation (100% recovery) of low‐molecular oxygen compounds from the remaining compounds, for which the recovery exceeds 95%. The obtained recoveries are satisfactory for preliminary separation of essential oils for analytical and preparative purposes.
Rapid determination of caffeoylquinic acid derivatives inCynara scolymusL. by ultra‐fast liquid chromatography/tandem mass spectrometry based on a fused core C18 column
Authors: Shen Qing , Dai Zhiyuan , Lu Yanbin 
Published: September 2010

AbstractAn ultra‐fast high‐performance LC‐ESI‐MS/MS method was developed for the analysis and quantification of caffeoylquinic acid derivatives, including chlorogenic acid, 1,3‐di‐O‐caffeoylquinic acid (cynarin) and 1,5‐di‐O‐caffeoylquinic acid, in artichoke (Cynara scolymusL.) heads and leaves. The rapid separation (less than 4 min) was achieved based on a Halo fused core C18‐silica column (50 mm×2.1 mm id, 2.7 μm). The target compounds were detected and quantified by a triple‐quadrupole mass spectrometer in multiple‐reaction monitoring mode. The calibration function is linear from 0.06 to 2800 ng/mL for chlorogenic acid, 0.3–3000 ng/mL for cynarin and 0.24–4800 ng/mL for 1,5‐di‐O‐caffeoylquinic acid, respectively. The average recoveries ranged from 92.1 to 113.2% with RSDs ≤6.5%. Moreover, four batches of artichoke head and leaf extracts were analyzed using the established method. The results indicated that the Halo fused core column provided much faster separations and higher sample throughput without sacrificing column ruggedness and reliability, and triple‐quadrupole MS provided extraordinarily lower LOQs for most of the target analytes. Comparing to conventional quantitative approaches, the established method was fast, sensitive and reliable for the determination of caffeoylquinic acid derivatives in artichoke.