bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protocol for the development of a tool to map systemic sclerosis pain sources, patterns, and management experiences: a Scleroderma Patient-centered Intervention Network patient-researcher partnership
Authors: Dal Santo Tiffany , Golberg Meira , Nassar Elsa-Lynn , Carrier Marie-Eve , Hu Sophie , Kwakkenbos Linda , Bartlett Susan J. , Fox Rina S. , Lee Yvonne C. , Varga John , Benedetti Andrea , Thombs Brett D. ,   the Scleroderma Patient-centered Intervention Network Pain Patient Advisory Team, Lapointe McKenzie Jo-Ann , Lawrie-Jones Amanda , Mieszczak Tracy , Petrozza Silvia , Sauve Maureen , Wixson Gayle 
Published: June 2024

Abstract
Introduction
Systemic sclerosis (SSc) is a rare, complex autoimmune rheumatic disease with multiple factors that contribute to pain. People with SSc emphasize the effect pain has on their quality of life, but no studies have systematically examined the frequency and relative importance of different SSc pain sources, patterns of pain from different sources, and pain management experiences. Our objectives are to (1) develop a tool, jointly with researchers, health care providers, and patients, to map sources of pain in SSc, determine patterns of pain from different sources, and understand pain management experiences; and (2) administer the final tool version to participants in the large multinational Scleroderma Patient-centered Intervention Network (SPIN) Cohort.

Methods
First, we will use validated pain assessment tools as templates to develop an initial version of our pain assessment tool, and we will obtain input from patient advisors to adapt it for SSc. The tool will include questions on pain sources, pain patterns, pain intensity, pain management techniques, and barriers to pain management in SSc. Second, we will conduct nominal group technique sessions with people living with SSc and health care providers who care for people with SSc to further refine the tool. Third, we will conduct individual usability testing sessions with SPIN Cohort participants. Once the tool has been finalized, we will administer it to individuals in the multinational SPIN Cohort, which currently includes over 1,300 active participants from 54 sites in 7 countries. We will perform unsupervised clustering using the KAy-Means for MIxed LArge data (KAMILA) method to identify participant subgroups with similar profiles of pain sources (present or absent) and to evaluate predictors of subgroup membership. We will use latent profile analysis to identify subgroups of participants with similar profiles based on pain intensity scores for each pain source and evaluate predictors.

Discussion
Once completed, our pain assessment tool will allow our team and other researchers to map sources of pain in SSc and to understand pain management experiences of people living with SSc. This knowledge will provide avenues for studies on the pathophysiology of pain in SSc and studies of interventions to improve pain management.
Improvement of insulin sensitivity by dietary fiber consumption during late pregnant sows is associated with gut microbiota regulation of tryptophan metabolism
Authors: Li Yang , He Jiaqi , Zhang Lijia , Liu Haoyu , Cao Meng , Lin Yan , Xu Shengyu , Che Lianqiang , Fang Zhengfeng , Feng Bin , Li Jian , Zhuo Yong , Wu De 
Published: June 2024

Abstract
Background
Dietary fiber (DF) consumption was reported to improve insulin sensitivity, change the tryptophan metabolism, and alter the gut microbiota. Herein, this study aimed to investigate the effects of DF consumption on insulin sensitivity, tryptophan metabolism, and gut microbiota composition in sows during late pregnancy, and explore the relationship between tryptophan metabolites and insulin sensitivity regulated by DF supplementation.

Results
Twelve sows were randomly assigned to two dietary treatment groups (six/group): the low-fiber (LF) group, which was fed a basal diet, and the high-fiber (HF) group, which was fed the basal diet supplemented with 22.60 g/kg inulin and 181.60 g/kg cellulose. During late pregnancy, meal test, glucose tolerance test, and insulin challenge test were used to investigate the insulin sensitivity of sows, using the percutaneous brachiocephalic vein catheterization technique. High DF consumption resulted in improved insulin sensitivity, especially during the second and third trimesters, and promoted serotonin production from tryptophan. Additionally, plasma serotonin concentration was positively correlated with the insulin sensitivity index during late pregnancy. Moreover, DF consumption elevated fecal short-chain fatty acid (SCFA) concentrations, altered fecal microbial diversity, and increased the abundances of Rikenellaceae_RC9_gut_group, Alloprevotella, Parabacteroides, Roseburia, and Sphaerochaeta, which were positively correlated to plasma serotonin concentration.

Conclusions
DF consumption improved insulin sensitivity during late pregnancy in sows, which improved microbial diversity in fecal samples and increased fecal SCFA concentrations, resulting in a positive correlation with plasma serotonin level.
Conservative femoral revision using short cementless stems with a tapered rectangular shape for selected Paprosky II–IV bone defects: an average seven-year follow-up
Authors: Li Yicheng , Zhang Xiaogang , Ji Baochao , Yushan Nuerailijiang , Wulamu Wuhuzi , Guo Xiaobin , Cao Li 
Published: June 2024

Abstract
Background
The use of long stems for severe femoral bone defects is suggested by many scholars, but it is associated with further bone loss, intraoperative fracture, increased surgical trauma, and complications. With better bone retention, simple and quick surgical procedures, and minimal complications, the short cementless stems with a tapered rectangular shape may be an alternative for femoral revision. This study aimed to evaluate the results of this type of stem in treating selected Paprosky II–IV bone defects.

Methods
This retrospective study included 73 patients (76 hips involved) who underwent conservative femoral revision using the short cementless stems with a tapered rectangular shape between January 2012 and December 2020. The preoperative femoral bone defects were identified as follows: 54 cases of type II, 11 cases of type IIIA, 7 cases of type IIIB, and 4 cases of type IV. Indications for revision included aseptic loosening (76.3%) and prosthetic joint infection (23.7%). Six cementless stems with a tapered rectangular shape from three companies were used in all patients. Among them, SLR-Plus, SL-Plus MIA, and Corail stems were employed in most patients (40.8%, 23.7%, and 17.1%, respectively). The average length of these stems measured 171.7 mm (SD 27 mm; 122–215 mm). Radiographic results, Harris hip scores (HHS), complications, and survivorship were analyzed. The follow-up lasted for 7 years on average (range 3–11 years).

Results
The subsidence was observed in three hips (3.9%), and all stems achieved stable bone ingrowth. Proximal femoral bone restoration in the residual osteolytic area was found in 67 hips (88.2%), constant defects in nine hips (11.8%), and increasing defects in 0 cases. There was no evidence of stem fractures and stem loosening in this series. The mean HHS significantly improved from 32 (range 15–50) preoperatively to 82 (range 68–94) at the last follow-up (t = − 36.297, P < 0.001). Five hips developed prosthesis-related complications, including three infection and two dislocation cases. The mean 5- and 10-year revision-free survivorships for any revision or removal of an implant and reoperation for any reason were 94.6% and 93.3%, respectively. Both mean 5- and 10-year revision-free survivorships for aseptic femoral loosening were 100%.

Conclusion
Conservative femoral revision using short cementless stems with a tapered rectangular shape can provide favorable radiographic outcomes, joint function, and mid-term survivorship with minimal complications. Of note, a sclerotic proximal femoral bone shell with continued and intact structure and enough support strength is the indication for using these stems.

Graphical Abstract
Genetic analysis of BRPF1 exon deletion variant causing intellectual developmental disorder with dysmorphic facies and ptosis in a Chinese family
Authors: Liu Qian , Li Feifei , Wang Nana , Fan Zhengjun 
Published: June 2024

Abstract
Background
Intellectual developmental disorders with dysmorphic facies and ptosis (IDDDFP) are rare neurological conditions caused by variants in the BRPF1 gene. They primarily manifest as intellectual disabilities (ID) alongside distinctive facial features, particularly ptosis and blepharophimosis. This study aimed to investigate the molecular etiology and phenotype of the inaugural IDDDFP family documented in China.

Methods and results
Clinical data were collected and validated through trio-based whole-exome sequencing of DNA from the proband and her parents, complemented by quantitative polymerase chain reaction (qPCR). The proband, a 10-month-old girl, presented with focal seizures and developmental delays. Notably, she exhibited facial features similar to those of her mother and sister, including ptosis and blepharophimosis. Both the proband’s mother and sister also had mild ID. Genetic testing identified BRPF1 deletion variants in all affected individuals, resulting in exon 2–14 heterozygous deletion. The qPCR verification confirmed the wild-type BRPF1 in the proband’s father and eldest sister. A review of 46 documented patients with BRPF1 deficiency revealed that the primary clinical manifestations encompassed varying degrees of ID alongside special facial features, skeletal deformities, and ocular abnormalities. However, epilepsy was found to be rare in this syndrome. The syndrome has variable phenotypic features of neurodevelopmental disorders. Meanwhile, there seems to be a lack of correlation between phenotype and genotype.

Conclusion
Our findings broaden the genotypic and phenotypic spectrum of individuals with genetically pathogenic variants of BRPF1. Moreover, they underscore the significance of recognizing ptosis and blepharophimosis associated with ID or seizures as potential signs of BRPF1 variants.
The fig (
Ficus carica
L.): varietal evolution from Asia to Puglia region, southeastern Italy
Authors: Mazzeo Andrea , Magarelli Andrea , Ferrara Giuseppe 
Published: June 2024

Abstract
The fig represents one of the oldest and widespread species in the Mediterranean basin, and thanks to man-made selections, it is possible to survey numerous varieties currently used for both main crop and brebas production. The fig is often associated with the birth of horticulture in Mediterranean and Near Asia areas. Figs were probably carried to southern Italy by Phoenicians and successively by Greeks and played an important role in Roman society. Puglia was in the past the most important region in southern Italy to produce figs and especially brebas (first crop), thanks to the excellent qualitative aspects of some varieties such as ‘Petrelli’ (syn. “Fiorone di S. Giovanni,” “Fiorone di S. Antonio”) and ‘Domenico Tauro’ that are mainly cultivated in the provinces of Bari and Brindisi. Main crop production, on the other hand, has always been based on the ‘Dottato’ variety, which was mainly used for drying and is partly consumed fresh, but also processed into jams and marmalades. There are numerous ‘minor’ varieties, which allow Puglia to be among the regions in the world with the greatest fig biodiversity, a kind of living repository grown during the centuries and in the last years rediscovered. However, the consumption and trade are exclusively at the local level, due to limited resistance to handling and shipping of such fruit; only a limited amount is destined to export. The main fig varieties cultivated in Puglia allow a wide ripening calendar starting from the first 10 days of June for brebas (often at the end of May) and ending in late September–October with late ripening varieties. The purpose of this mini review is to highlight the high degree of biodiversity that exists in Puglia region since many centuries, and the importance that this ancient crop could have in modern fruit growing, as it was once a key player in market scenarios in many Mediterranean and Near Asia countries. The fig biodiversity could be used either for fresh and processed consumption or for breeding programs for new varieties with desired traits and qualities.
Inhibition of cysteine-serine-rich nuclear protein 1 ameliorates ischemia–reperfusion injury during liver transplantation in an MAPK-dependent manner
Authors: Wang Zhoucheng , Ge Wenwen , Zhong Xinyang , Tong Shizheng , Zheng Shusen , Xu Xiao , Wang Kai 
Published: June 2024

AbstractHepatic ischemia–reperfusion injury (HIRI) is a critical pathophysiological process during liver transplantation (LT). Multiple genes and signal pathways are dysregulated during HIRI. This study aims to identify genes as potential therapeutic targets for ameliorating HIRI. Datasets containing samples from the human donor liver (GSE151648) and mouse HIRI model (GSE117066) were analyzed to determine differentially expressed genes (DEGs). The selected DEGs were confirmed by real-time PCR and western blot in the hepatocyte hypoxia-reoxygenation (HR) model, mouse HIRI model, and human liver samples after transplantation. Genetic inhibition was used to further clarify the underlying mechanism of the gene in vitro and in vivo. Among the DEGs, CSRNP1 was significantly upregulated (|log FC|= 2.08, P < 0.001), and was positively correlated with the MAPK signal pathway (R = 0.67, P < 0.001). CSRNP1 inhibition by siRNA significantly suppressed apoptosis in the AML-12 cell line after HR (mean Annexin+ ratio = 60.62% vs 42.47%, P = 0.0019), but the protective effect was eliminated with an additional MAPK activator. Knocking down CSRNP1 gene expression by intravenous injection of AAV-shRNA markedly reduced liver injury in mouse HIRI model (ALT: AAV-NC vs AAV-shCsrnp1 = 26,673.5 ± 2761.2 vs 3839.7 ± 1432.8, P < 0.001; AST: AAV-NC vs AAV-shCsrnp1 = 8640.5 ± 1450.3 vs 1786.8 ± 518.3, P < 0.001). Liver-targeted delivery of siRNA by nanoparticles effectively inhibited intra-hepatic genetic expression of Csrnp1 and alleviated IRI by reducing tissue inflammation and hepatocyte apoptosis. Furthermore, CSRNP1 inhibition was associated with reduced activation of the MAPK pathway both in vitro and in vivo. In conclusion, our results demonstrated that CSRNP1 could be a potential therapeutic target to ameliorate HIRI in an MAPK-dependent manner.
Monitoring and enhancing spontaneous sustainability—a framework
Authors: Hassan Gehan I. , Ettouney Sayed M. , Abdel-Kader Nasamat M. 
Published: June 2024

AbstractThe paper presents and addresses the concept of “spontaneous sustainability”, the informal and unplanned sustainable development of communities, as the basis and framework to support the notion, drives, and agendas of “formal sustainability”. It follows the interdependence between the “spontaneous sustainability” of “distinct” communities and their “cultural” characteristics, including history, spatial continuity, values and behaviors, the associated physical settings, and tangible and intangible products, which enabled them to continue, survive, and develop. The research aims at formulating and validating a “framework” for monitoring and enhancing the “spontaneous sustainability” of “distinct” communities to enable, support and link it, to the broader frameworks of “sustainable development”. The proposed “framework” combines “sustainability” dimensions: environmental, economic, and socio-cultural; and the levels of community contexts, and spatial impact. The “framework” is formulated by integrating selected “urban sustainability” approaches, and systems, with later research that developed the key aspects, criteria, and indicators of those systems, together with research addressing “social and cultural sustainability”, and assessment aspects, criteria, and indicators. The “framework” is developed and validated through a limited questionnaire, involving local experts and specialists, academics, and practitioners, to point out limitations and potential, and to suggest the relative importance of its components, key aspects, and criteria. The validation supported the research propositions, the formulation, and development of the proposed “spontaneous sustainability framework” and emphasized its flexibility to include other approaches and agendas, and potential for further development and application in distinct local communities.
Maladaptive personality traits are associated with burnout risk in Italian anesthesiologists and intensivists: a secondary analysis from a cross-sectional study
Authors: Vittori Alessandro , Petrucci Emiliano , Cascella Marco , Bignami Elena Giovanna , Simonini Alessandro , Sollecchia Giacomo , Fiore Gilberto , Vergallo Alessandro , Marinangeli Franco , Pedone Roberto 
Published: June 2024

Abstract
Background
Burnout is a maladaptive response to chronic stress, particularly prevalent among clinicians. Anesthesiologists are at risk of burnout, but the role of maladaptive traits in their vulnerability to burnout remains understudied.

Methods
A secondary analysis was performed on data from the Italian Association of Hospital Anesthesiologists, Pain Medicine Specialists, Critical Care, and Emergency (AAROI-EMAC) physicians. The survey included demographic data, burnout assessment using the Maslach Burnout Inventory (MBI) and subscales (emotional exhaustion, MBI-EE; depersonalization, MBI-DP; personal accomplishment, MBI-PA), and evaluation of personality disorders (PDs) based on DSM-IV (Diagnostic and Statistical Manual of Mental Disorders Fourth Edition) criteria using the assessment of DSM-IV PDs (ADP-IV). We investigated the aggregated scores of maladaptive personality traits as predictor variables of burnout. Subsequently, the components of personality traits were individually assessed.

Results
Out of 310 respondents, 300 (96.77%) provided complete information. The maladaptive personality traits global score was associated with the MBI-EE and MBI-DP components. There was a significant negative correlation with the MBI-PA component. Significant positive correlations were found between the MBI-EE subscale and the paranoid (r = 0.42), borderline (r = 0.39), and dependent (r = 0.39) maladaptive personality traits. MBI-DP was significantly associated with the passive-aggressive (r = 0.35), borderline (r = 0.33), and avoidant (r = 0.32) traits. Moreover, MBI-PA was negatively associated with dependent (r =  − 0.26) and avoidant (r =  − 0.25) maladaptive personality features.

Conclusions
There is a significant association between different maladaptive personality traits and the risk of experiencing burnout among anesthesiologists. This underscores the importance of understanding and addressing personality traits in healthcare professionals to promote their well-being and prevent this serious emotional, mental, and physical exhaustion state.
Coverage and Socioeconomic Inequalities in Cervical Cancer Screening in Low- and Middle-Income Countries Between 2010 and 2019
Authors: Abila Derrick Bary , Wasukira Sulaiman B. , Ainembabazi Provia , Kiyingi Elizabeth Nakiyingi , Chemutai Beliza , Kyagulanyi Eddy , Varsani Jaimin , Shindodi Beatha , Kisuza Ruth Ketty , Niyonzima Nixon 
Published: June 2024

PURPOSE Cervical cancer screening is vital in addressing the global burden of cervical cancer. In this study, we describe the coverage and socioeconomic inequalities in the coverage of cervical cancer screening in low- and middle-income countries (LMICs). METHODS We analyzed data from the women's recode files of the Demographic and Health Surveys conducted in LMICs from 2010 to 2019 with variables on cervical cancer screening. We included women 21 years or older and determined the proportion of women who were screened for cervical cancer by age categories, wealth quintile, type of place of residence, level of education, and marital status. Socioeconomic inequality was measured using the concentration index (CIX) and the slope index of inequality (SII). RESULTS A total of 269,506 women from 20 surveys in 16 countries were included in the survey. Generally, there was a low coverage of screening, with lower rates among women age 21-24 years, living in rural areas, in the poorest wealth quintile, with no formal education, and who have never been in union with or lived with a man. The CIX and SII values for screening for cervical cancer were positive (pro-rich) for all the countries except Tajikistan in 2012 where they were negative (pro-poor). CONCLUSION The coverage of cervical cancer screening was low in LMICs with variations by the quintile of wealth (pro-rich) and type of place of residence (pro-urban). To achieve the desired impact of cervical cancer screening services in LMICs, the coverage of cervical cancer screening programs must include women irrespective of the type of place and wealth quintiles.
Stigma in Pediatric Cancer: An Exploratory Study of Osteosarcoma and Retinoblastoma in Guatemala, Jordan, and Zimbabwe
Authors: Graetz Dylan E. , Velasquez Thelma , Chitsike Inam , Halalsheh Hadeel , Cáceres-Serrano Ana , Fuentes Lucia , Chokwenda Nester , Matsikidze Edith , Ferrara Gia , Bilbeisi Tharwa , Williams Anneliese , Bhakta Nickhill , Jeha Sima , Rodriguez Galindo Carlos , Mack Jennifer W. , Santana Victor M. 
Published: June 2024

PURPOSE Stigma is an understudied barrier to health care acceptance in pediatric oncology. We sought to explore the stigma experience, including its impact on cancer treatment decision making, and identify strategies to mitigate stigma for patients with osteosarcoma and retinoblastoma in Guatemala, Jordan, and Zimbabwe. METHODS Participants included caregivers, adolescent patients (age 12-19 years), and health care clinicians. A semistructured interview guide based on The Health Stigma and Discrimination Framework (HSDF) was adapted for use at each site. Interviews were conducted in English, Spanish, Arabic, or Shona, audio-recorded, translated, and transcribed. Thematic analysis focused on stigma practices, experiences, outcomes, drivers, mitigators, and interventions. RESULTS We conducted 56 interviews (28 caregivers, 19 health care clinicians, nine patients; 20 in Guatemala, 21 in Jordan, 15 in Zimbabwe). Major themes were organized into categories used to adapt the HSDF to global pediatric cancer care. Themes were described similarly across all sites, ages, and diagnoses, with specific cultural nuances noted. Pediatric cancer stigma was depicted as an isolating and emotional experience beginning at diagnosis and including internalized and associative stigma. Stigma affected decision making and contributed to negative outcomes including delayed diagnosis, treatment abandonment, regret, and psychosocial fragility. Overcoming stigma led to positive outcomes including resilience, treatment adherence, pride, and advocacy. Identified stigma drivers and mitigators were linked to potential interventions. CONCLUSION Participants describe a shared stigma experience that transcends geography, cultural context, age, and diagnosis. Stigma manifestations have the potential to impact medical decision making and affect long-term psychological outcomes. Stigma assessment tools and interventions aimed at stigma mitigation including educational initiatives and support groups specific to pediatric cancer should be the focus of future research.