bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of antihistamine and anti‐inflammatory medication use on risk of specific glioma histologies
Authors: Scheurer Michael E. , Amirian E. Susan , Davlin Stacy L. , Rice Terri , Wrensch Margaret , Bondy Melissa L. 
Published: December 2010

AbstractSeveral studies have shown a decrease in glioma risk associated with a personal history of allergic conditions and the medications used to treat the symptoms. However, few studies have been able to examine risk within histological subgroups of glioma. Case–control data from M. D. Anderson Cancer Center and University of California, San Francisco were pooled to conduct the analysis stratified by histological subtype. A risk prediction model considering inflammation‐related variables and antihistamine use was built using logistic regression. Of the subtypes examined, long‐term antihistamine use was associated with increased risk of anaplastic gliomas, especially when length of use was considered in conjunction with history of asthma or allergy. Anaplastic cases with no history of asthma or allergy were 2.94 times more likely than controls to report antihistamine use for 10 years or more; whereas anaplastic cases with a history of asthma or allergy were 2.34 times more likely than controls to report antihistamine use for 10 years or more. Furthermore, anti‐inflammatory medication use was associated with a protective effect against glioblastoma (OR = 0.80; 95% CI: 0.65, 0.99), especially among individuals with no history of asthma or allergies. No statistically significant effects of anti‐inflammatory drugs or antihistamines were evident for the other histological subtypes. Thus, modulation of the immune system by the use of common drugs, such as antihistamines or nonsteroidal anti‐inflammatory drugs, may contribute to the development of certain types of brain tumors.
Long‐term persistence of systemic and mucosal immune response to HPV‐16/18 AS04‐adjuvanted vaccine in preteen/adolescent girls and young women
Authors: Petäjä Tiina , Pedersen Court , Poder Airi , Strauss Gitte , Catteau Gregory , Thomas Florence , Lehtinen Matti , Descamps Dominique 
Published: December 2010

AbstractVaccination against oncogenic human papillomavirus (HPV) types is one key intervention for cervical cancer prevention. This follow‐up study assessed the persistence of the systemic and mucosal immune responses together with the safety profile of the HPV‐16/18 AS04‐adjuvanted vaccine administered to young women aged 10–25 years. Serum and cervicovaginal secretion (CVS) samples were collected at prespecified time‐points during the 48‐month follow‐up period. Anti‐HPV‐16/18 antibody levels in serum and CVS were measured by enzyme‐linked immunosorbent assay (ELISA). At Month 48, all subjects remained seropositive for serum anti‐HPV‐16 and ‐18 antibodies. As previously observed, anti‐HPV‐16 and ‐18 antibodies levels (ELISA Units/mL) were higher in subjects vaccinated at the age of 10–14 years (2862.2 and 940.8) compared to subjects vaccinated at the age of 15–25 years (1186.2 and 469.8). Moreover, anti‐HPV‐16 and ‐18 antibodies in CVS were still detectable for subjects aged 15–25 years (84.1% and 69.7%, respectively). There was a strong correlation between serum and CVS anti‐HPV‐16 and ‐18 antibodies levels (correlation coefficients = 0.84 and 0.90 at Month 48, respectively) supporting the hypothesis of transudation or exudation of serum immunoglobulin G antibodies through the cervical epithelium. The HPV‐16/18 AS04‐adjuvanted vaccine had a clinically acceptable safety profile. In conclusion, this follow‐up study shows that the HPV‐16/18 AS04‐adjuvanted vaccine administered to preteen/adolescents girls and young women induces long‐term systemic and mucosal immune response and has a clinically acceptable safety profile up to 4 years after the first vaccine dose.
Biochemical studies on Helicobacter pylori arginase: Insight into the difference in activity compared to other arginases
Authors: Srivastava Abhishek , Sau Apurba Kumar 
Published: December 2010

AbstractArginase is a binuclear Mn2+‐metalloenzyme of urea cycle that catalyzes the conversion of L‐arginine to L‐ornithine and urea. Unlike other arginases, the Helicobacter pylori enzyme is selective for Co2+, and has lower catalytic activity. To understand the differences in the biochemical properties as well as activity compared to other arginases, we carried out a detailed investigation of different metal reconstituted H. pylori arginases that includes steady‐state kinetics, fluorescence measurement, pH‐dependent and oligomerization assays. Unlike other arginases (except human at physiological pH), the Co2+‐ and Mn2+‐reconstituted H. pylori enzymes exhibit cooperative mechanism of arginine hydrolysis, and undergo self‐association and activation with increasing concentrations. Analytical gel‐filtration assays in conjunction with the kinetic data showed that the protein exists as a mixture of monomer and dimer with monomer being the major form (other arginases exclusively exist as a trimer or hexamer) but the dimer is associated with higher catalytic activity. The proportion of dimer is found to decrease with increasing salt concentrations indicating that salt bridges play important roles in dimerization of the protein. Furthermore, the fluorescence measurement showed that Co2+ ions play an important role in the local tertiary structure of the protein than Mn2+. This is consistent with the pH‐dependent studies where the Co2+‐enzyme showed a single ionization compared to the double in the Mn2+‐enzyme. Thus, this study presents the detailed biochemical and spectroscopic investigations into the differences in the biochemical properties and activity between H. pylori and other arginases. © 2010 IUBMB IUBMB Life, 62(12): 906–915
A role for human neuroglobin in apoptosis
Authors: Brittain Thomas , Skommer Joanna , Henty Kristen , Birch Nigel , Raychaudhuri Subhadip 
Published: December 2010

AbstractOver the past decade, following the discovery of the human heme protein neuroglobin, many studies have searched for evidence for this protein's mechanism of action. Much data has accrued showing that high levels of neuroglobin will protect cells from apoptotic cell death, following a wide range of challenges. Various explanations of its actions, based on measured reactivity with oxygen, nitric oxide, or free radicals, have been proposed, but none have, as yet, been substantiated in vivo. Following preliminary experiments, it was previously hypothesised that “the central role of neuroglobin in highly metabolically active cells and retinal and brain neurons is to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis” (I.U.M.B.M. Life (2008) 60, 398). In this article, we review the evidence, which has accumulated to support this hypothesised mechanism of action of neuroglobin and integrate this data, with other reported intracellular functions of neuroglobin, to suggest a plausible central role for neuroglobin in the control of apoptosis. © IUBMB IUBMB Life, 62(12): 878–885, 2010.
Toxicological responses of Cyprinus carpio exposed to the herbicide penoxsulam in rice field conditions
Authors: Cattaneo Roberta , Clasen Bárbara , Lucia Loro Vania , de Menezes Charlene Cavalheiro , Moraes Bibiana , Santi Adriana , Toni Candida , de Avila Luis Antonio , Zanella Renato 
Published: December 2010

ABSTRACTCyprinus carpio fish were exposed to penoxsulam (Ricer) in field conditions. The experiment in the rice field was carried out for 7, 21 and 72 days. Oxidative stress parameters and antioxidant profile were studied. The acetylcholinesterase (AChE) enzyme activity in the brain was increased after 7 days and reduced after 21 and 72 days of the experiment in the rice field. The AChE activity in muscle was reduced only after 72 days of exposure. Thiobarbituric acid‐reactive species were increased in the liver, brain and muscle at 7 days of the trial, reduced at 21 days in the brain and unaltered after 72 days of exposure in muscle. However, an increase in this parameter in the brain and liver was observed. Liver glutathione S‐transferase was reduced at 7 days, unchanged at 21 days and increased after 72 days of exposure. Catalase of the liver changed only in the second experimental period, when it was reduced. Liver protein carbonyl was reduced at 7 days and increased at 21 and 72 days of exposure. This study shows long‐term effects of rice herbicide at environmentally relevant concentrations on toxicological parameters in different tissues (brain, muscle and liver) of Cyprinus carpio. Copyright © 2010 John Wiley & Sons, Ltd.
Agreement between pQCT- and DXA-derived indices of bone geometry, density, and theoretical strength in females of varying age, maturity, and physical activity
Authors: Dowthwaite Jodi Noelle , Flowers Portia PE , Scerpella Tamara Ann 
Published: December 2010

Abstract
Measurement of bone mass, geometry, density, and strength are critical in bone research and clinical studies. For peripheral quantitative computed tomography (pQCT), single and repeated measurements are particularly adversely affected by movement and positional variation. Dual-energy X-ray absorptiometry (DXA)–derived indices may alleviate these problems and provide useful alternative assessments. To evaluate this hypothesis, distal radius DXA and pQCT indices were compared in 101 healthy females aged 8.0 to 22.8 years (prepuberty to adulthood), reflecting a broad range of body sizes, physical maturity, and activity exposures. At the diaphysis, correlations were ρ = +0.74 to +0.98, with strong intermethod agreement for most indices. At the metaphysis, correlations were ρ = +0.64 to +0.97; intermethod agreement improved with modifications to the simplified geometric formulas more closely reflecting metaphyseal bone geometry. Further improvements may be possible because skeletal size and maturity-related biases in agreement were detected. Overall, DXA-derived indices may provide a useful assessment of bone geometry, density, and theoretical strength contingent on appropriate consideration of their limitations. © 2011 American Society for Bone and Mineral Research.
Effects of repetitive loading on the growth-induced changes in bone mass and cortical bone geometry: A 12-month study in pre/peri- and postmenarcheal tennis players
Authors: Ducher Gaele , Bass Shona L , Saxon Leanne , Daly Robin M 
Published: December 2010

Abstract
Pre- and early puberty may be the most opportune time to strengthen the female skeleton, but there are few longitudinal data to support this claim. Competitive female premenarcheal (pre/peri, n = 13) and postmenarcheal (post, n = 32) tennis players aged 10 to 17 years were followed over 12 months. The osteogenic response to loading was studied by comparing the playing and nonplaying humeri for dual-energy X-ray absorptiometry (DXA) bone mineral content (BMC) and magnetic resonance imaging (MRI) total bone area (ToA), medullary area (MedA), cortical area (CoA), and muscle area (MCSA) at the humerus. Over 12 months, growth-induced gains (nonplaying arm) in BMC, ToA, and CoA were greater in pre/peri (10% to 19%, p &lt; .001) than in post (3% to 5%, p &lt; .05 to .001) players. At baseline, BMC, ToA, CoA, and MCSA were 8% to 18% greater in the playing versus nonplaying arms in pre/peri and post players (all p &lt; .001); MedA was smaller in the playing versus nonplaying arms in post only players (p &lt; .05). When comparing the annual gains in the playing arm relative to changes in the nonplaying arm, the increases in ToA and CoA were greater in pre/peri than post players (all p &lt; .05). The smaller the side-to-side differences in BMC and CoA at baseline, the larger the exercise benefits at 12 months (r = −0.39 to −0.48, p &lt; .01). The exercise-induced change in MCSA was predictive of the exercise benefits in BMC in pre/peri players only (p &lt; .05). In conclusion, both pre/peri- and postmenarcheal tennis players showed significant exercise-induced skeletal benefits within a year, with greater benefits in cortical bone geometry in pre/perimenarcheal girls. © 2011 American Society for Bone and Mineral Research.
Preventive effect of phytoglycoprotein (27 kDa) on inflammatory factors at liver injury in cadmium chloride‐exposed ICR mice
Authors: Lee Jin , Lim Kye‐Taek 
Published: December 2010

AbstractCadmium is one of the inflammation‐related xenobiotics and has been regarded as a potent carcinogen. Gardenia jasminoides Ellis (GJE) has been used to cure inflammation in Korean folk medicine for a long time. The purpose of present study is the inhibitory effect of glycoprotein isolated from GJE (27 kDa) on inflammation mechanism in cadmium chloride‐exposed ICR mice. We evaluated the activities of lactate dehydrogenase (LDH), alanine aminotransferase (ALT), and thiobarbituric acid‐reactive substances (TBARS), activities of anti‐oxidative enzymes [superoxide dismutase (SOD) and gluthathione peroxidase (GPx)], activities of c‐Jun N‐terminal protein kinase (JNK), heat shock protein 27 (Hsp27), activator protein (AP)‐1, nuclear factor (NF)‐κB and expression of inflammation‐related mediators including tumor necrosis factor (TNF)‐α and interleukin (IL)‐6 in cadmium chloride‐exposed ICR mice using immunoblot analysis, EMSA and RT‐PCR. It notes that mice plasma was used to measure ALT, LDH, and TBARS after treatment with cadmium chloride alone or cadmium chloride under the pretreatment with GJE glycoprotein. Liver tissues were used to assess activities of anti‐oxidant enzymes, SAPK/JNK, Hsp27, AP‐1, NF‐κB, TNF‐α, and IL‐6 in this study. The results obtained from this study revealed that GJE glycoprotein (10 mg/kg) decreased the levels of LDH, ALT and TBARS, whereas increased the activity of hepatic anti‐oxidant enzymes (SOD and GPx) in cadmium chloride‐exposed ICR mice. Moreover, it decreased the activity of JNK/AP‐1, NF‐κB, Hsp27, and pro‐inflammatory cytokines (TNF‐α and IL‐6). Taken together, the results in this study suggest that GJE glycoprotein inhibits the expression of inflammation‐related cytokines (TNF‐α and IL‐6) in cadmium chloride‐exposed ICR mice. J. Cell. Biochem. 112: 694–703, 2011. © 2010 Wiley‐Liss, Inc.
Expression and regulation of B7‐H3 immunoregulatory receptor, in human mesothelial and mesothelioma cells: Immunotherapeutic implications
Authors: Calabrò Luana , Sigalotti Luca , Fonsatti Ester , Bertocci Erica , Di Giacomo Anna Maria , Danielli Riccardo , Cutaia Ornella , Colizzi Francesca , Covre Alessia , Mutti Luciano , Natali Pier Giorgio , Maio Michele 
Published: December 2010

AbstractNo treatment prolongs the survival of malignant mesothelioma (MM) patients. Since MM elicits anti‐tumor host's immune responses, immunotherapy represents a promising strategy for its control. Immunomodulatory antibodies against components of the B7 family of immunomodulatory molecules that regulate T cell activation are being investigated in human malignancies including MM. The expression of B7‐H3, a new component of the B7 family was investigated in primary cultures of human mesothelial cells (HMC) and in MM cell lines by flow cytometry and molecular analyses, and in MM tissues by immunohistochemistry. The role of DNA hypomethylating agents in modulating levels of B7‐H3 expression in MM cells was also studied. Reverse transcriptase‐polymerase chain reaction (RT‐PCR) demonstrated that B7‐H3 mRNA was consistently detectable in mesothelial and MM cells investigated; however, real‐time quantitative RT‐PCR analyses showed highly heterogeneous levels of B7‐H3 mRNA among investigated MM cells. The analysis of B7‐H3 protein expression indicated that comparable levels of B7‐H3 were expressed on both cell types. Treatment with the DNA hypomethylating agent 5‐aza‐2′‐deoxycytidine did not significantly affect the expression of B7‐H3 mRNA in MM cells. In vivo, while B7‐H3 was expressed in all 13 tumor biopsies of the epithelial variant, with high levels in 54% of cases, it was rarely detectable in spindle type MM in which 1/5 biopsies weakly expressed B7‐H3. These findings suggest that B7‐H3 is a promising target for new immunotherapeutic strategies in MM, with particular emphasis in the epithelial variant. J. Cell. Physiol. 226: 2595–2600, 2011. © 2010 Wiley‐Liss, Inc.
Role of Excitatory Amino Acid Transporter‐2 (EAAT2) and glutamate in neurodegeneration: Opportunities for developing novel therapeutics
Authors: Kim Keetae , Lee Seok‐Geun , Kegelman Timothy P. , Su Zhao‐Zhong , Das Swadesh K. , Dash Rupesh , Dasgupta Santanu , Barral Paola M. , Hedvat Michael , Diaz Paul , Reed John C. , Stebbins John L. , Pellecchia Maurizio , Sarkar Devanand , Fisher Paul B. 
Published: December 2010

AbstractGlutamate is an essential excitatory neurotransmitter regulating brain functions. Excitatory amino acid transporter (EAAT)‐2 is one of the major glutamate transporters expressed predominantly in astroglial cells and is responsible for 90% of total glutamate uptake. Glutamate transporters tightly regulate glutamate concentration in the synaptic cleft. Dysfunction of EAAT2 and accumulation of excessive extracellular glutamate has been implicated in the development of several neurodegenerative diseases including Alzheimer's disease, Huntington's disease, and amyotrophic lateral sclerosis. Analysis of the 2.5 kb human EAAT2 promoter showed that NF‐κB is an important regulator of EAAT2 expression in astrocytes. Screening of approximately 1,040 FDA‐approved compounds and nutritionals led to the discovery that many β‐lactam antibiotics are transcriptional activators of EAAT2 resulting in increased EAAT2 protein levels. Treatment of animals with ceftriaxone (CEF), a β‐lactam antibiotic, led to an increase of EAAT2 expression and glutamate transport activity in the brain. CEF has neuroprotective effects in both in vitro and in vivo models based on its ability to inhibit neuronal cell death by preventing glutamate excitotoxicity. CEF increases EAAT2 transcription in primary human fetal astrocytes through the NF‐κB signaling pathway. The NF‐κB binding site at −272 position was critical in CEF‐mediated EAAT2 protein induction. These studies emphasize the importance of transcriptional regulation in controlling glutamate levels in the brain. They also emphasize the potential utility of the EAAT2 promoter for developing both low and high throughput screening assays to identify novel small molecule regulators of glutamate transport with potential to ameliorate pathological changes occurring during and causing neurodegeneration. J. Cell. Physiol. 226: 2484–2493, 2011. © 2010 Wiley‐Liss, Inc.
Tenascin‐C enhances crosstalk signaling of integrin αvβ3/PDGFR‐β complex by SRC recruitment promoting PDGF‐induced proliferation and migration in smooth muscle cells
Authors: Ishigaki Tomoki , Imanaka‐Yoshida Kyoko , Shimojo Naoshi , Matsushima Satoshi , Taki Waro , Yoshida Toshimichi 
Published: December 2010

AbstractMigration and proliferation of smooth muscle cells (SMCs) are key events during neointimal formation in pathological conditions of vessels. Tenascin‐C (TNC) is upregulated in the developing neointima of lesions. We evaluated the effects of TNC on responses of SMCs against platelet‐derived growth factor (PDGF) stimulation. TNC coated on substrate promoted PDGF‐BB‐induced proliferation and migration of rat SMC cell line A10 in BrdU incorporation and transwell assays, respectively. Immunoblotting showed that TNC substrate enhanced autophosphorylation of PDGFR‐β after PDGF‐BB stimulation. Integrin αvβ3 is known to be a receptor for TNC in SMCs. In immunofluorescence and immunoblot of integrin αv subunit, clustering of αv‐positive focal adhesions and upregulated αv expression were observed in the cells on TNC substrate. Immunoprecipitation using anti‐integrin αvβ3 antibody demonstrated that PDGFR‐β and integrin αvβ3 were co‐precipitated and that the relative amount of PDGFR‐β after the stimulation was increased by TNC treatment. TNC also promoted phosphorylation of focal adhesion kinase (FAK) at tyrosine (Y) 397 and Y925. The phosphorylated FAK was localized at focal adhesions in immunofluorescence. Phosphorylated SRC at Y418 was also seen at focal adhesions. Immunoprecipitation with αv antibody showed increased SRC association with the integrin signaling complex in the cells on TNC after PDGF treatment. In the cells on TNC substrate, crosstalk signaling between integrin αvβ3 and PDGFR‐β could be amplified by SRC and FAK recruited to focal adhesions, followed by enhanced proliferation and migration of A10 cells by PDGF‐BB. J. Cell. Physiol. 226: 2617–2624, 2011. © 2010 Wiley‐Liss, Inc.
Ultrasonographic measures of subacromial space in patients with rotator cuff disease: A systematic review
Authors: Seitz Amee L. , Michener Lori A. 
Published: December 2010

AbstractPurpose.Recently sonography (US) has been used to measure the subacromial space outlet with a linear measurement of the acromiohumeral distance (AHD). The purpose of this article is to systematically review the literature on the influence of rotator cuff disease (RCD) on AHD using US.Methods.Computer‐aided searches of databases were performed to identify comparative studies that measured US‐generated AHD in adults with symptomatic RCD.Results.Five studies met the inclusion/exclusion criteria.Conclusions.US images of AHD are smaller in patients with full‐thickness tears than healthy individuals and subjects with subacromial impingement. AHD is potentially useful to prognosticate outcome in patients with subacromial impingement. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound, 2011
Changing predictors of spatial and temporal variability in stocking rates in a severely degraded communal rangeland
Authors: Vetter S. , Bond W. J. 
Published: December 2010

AbstractChanges in vegetation and soils under heavy continuous grazing have been widely documented, but the effects of these on secondary production are debated. Sterkspruit is one of South Africa's most degraded districts, and soil erosion in the district increased substantially over the twentieth century. Despite this, records dating to the late‐1800s show no decline in livestock numbers. This study explores how high stocking rates have been maintained despite an apparent deterioration in the natural resource base. Multiple regression analyses were used to determine the best predictors of stocking rates in 1974, the 1980s and 1997 to determine whether the relative importance of mean annual rainfall and winter temperature (indicative of primary production), the percentage of area on nutrient‐poor sandstone‐derived soils (representing a dry season nutritional bottleneck), the percentage of an area on steep slopes (which make areas less accessible to livestock) and human population density and availability of arable land (indicative of potential inputs) had changed over time. While mean annual rainfall was the best predictor of stocking rates in 1974, stocking rates in the 1980s and 1997 were best predicted by human population densities. This suggests that a qualitative shift in how livestock populations are maintained has taken place. Copyright © 2010 John Wiley & Sons, Ltd.