bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional heterogeneity of non‐small lung adenocarcinoma cell sub‐populations
Authors: Bechyne Iga , Szpak Katarzyna , Madeja Zbigniew , Czyż Jarosław 
Published: September 2011

AbstractThe morphological and functional heterogeneity of solid tumour cells can be observed in cancer cell lines cultured in vitro. We have combined analyses of microclones developed from single cells with micropore transmigration assays to demonstrate the co‐existence of cellular subsets differing in morphology and motile activity, as well as Cx43 (connexin 43) and N‐cadherin expression within lung carcinoma A549 populations. ‘Fibroblastoid’ cells, characterized by high motility, polarized morphology and plasmalemmal localization of Cx43, displayed the strongest aptitude for transmigration through narrow obstacles. Due to high mitotic activity, they maintain the whole population but can also give rise to a sub‐population of quiescent and immobile ‘epithelioid’ cells. Our observations indicate that phenotypic transitions between the fibroblastoid and epithelioid phenotype account for the heterogeneity of metastable A549 cell populations.
Enhanced Differentiation of Human Adipose Tissue-derived Stromal Cells into Insulin-producing Cells with Glucagon-like Peptide-1
Authors: Wei A.-H. , Wang W.-J. , Mu X.-P. , Li H.-M. , Yan W.-Q. 
Published: September 2011

AbstractType 1 diabetes mellitus (T1DM) is mainly caused by reduction of the endogenous insulin secretion due to autoimmune destruction of pancreatic β cells, and a promising therapeutic approach for T1DM is pancreas and islet cell replacement. The major obstacle is the limited source of insulin-producing cells. Here, we report an efficient approach to induce human adipose-derived stromal cells (hADSCs) to differentiate into insulin-producing cells, with glucagon-like peptide-1 (GLP-1). hADSCs were successfully isolated from the adipose tissue, with adipogenic and osteogenic differentiation potency. Islet-like cell clusters formed in the culture, which was enhanced with the treatment of GLP-1. Reverse transcription polymerase chain reaction analysis showed the expression of the pancreas-related genes in the differentiated cells, such as pdx-1, ngn3, insulin, glucagon, somatostatin, glucokinase n and glut2. Immunocytochemical analysis showed that the induced cells co-expressed insulin, C-peptide and PDX-1. The GLP-1 receptor was present in the differentiated cells. In addition, flow cytometry analysis and ELISA showed that, in the presence of GLP-1, the percentage of insulin-producing cells was increased from 5.9% to 28.0% and the release of insulin increased from 9.53±0.7 pmol/106 cells to 15.86±1.3 pmol/106 cells. Insulin was released in response to glucose stimulation in a manner comparable to that of adult human islets. These results indicated that hADSCs isolated from adipose tissues can be induced to differentiate into insulin-producing cells, which is further enhanced with the treatment of GLP-1. These findings confirm that the differentiation of hADSCs to insulin-producing cells is indeed possible and indicate that the differentiated insulin-producing cells can be used as a potential source for transplantation into patients with T1DM.
ERK1/2 MAPKs and Wnt Signaling Pathways are Independently Involved in Adipocytokine-Mediated Aldosterone Secretion
Authors: Vleugels K. , Schinner S. , Krause D. , Morawietz H. , Bornstein S. , Ehrhart-Bornstein M. , Krug A. 
Published: September 2011

AbstractObesity is one major risk factor for the development of arterial hypertension, and the development of obesity-related hypertension has been associated with increased plasma aldosterone levels. Our previous work shows a direct stimulatory effect of adipokines on aldosterone secretion from human adrenocortical cells, mediated via ERK1/2-dependent upregulation of steroid acute regulatory protein (StAR) activity. Recent evidence also indicates the involvement of the Wnt-signaling pathway in fat cell-mediated aldosterone secretion. Wnt-signaling molecules are secreted by adipocytes and regulate the activity of SF-1, a key transcription factor in adrenal steroidogenesis. The goal of this study was to investigate the cellular mechanisms of adipocyte-induced aldosterone secretion in detail, and to evaluate effects and possible interactions of the ERK1/2 MAPK- and the Wnt-signaling pathways on adipocyte-induced adreno­cortical aldosterone secretion. Our results show that, similar to adipocyte-conditioned medium (ACM), β-catenin, which is an intracellular mediator of canonical Wnt-signaling, induced StAR promotor activity in human NCI-H295R adrenocortical cells, and ACM-induced StAR promotor activity depended on intact SF-1 binding sites. Wnt antagonist sFRP-1 inhibited adipokine-mediated StAR activity, but did not affect ERK1/2 MAPK activation. Accordingly, Wnt did not stimulate ERK1/2 phosphorylation in adrenocortical cells, indicating that ERK1/2 MAPK and Wnt signaling pathways are independently involved in adipocyte-mediated aldosterone secretion.
Metformin after Bariatric Surgery – an Acid Problem
Authors: Aberle J. , Reining F. , Dannheim V. , Flitsch J. , Klinge A. , Mann O. 
Published: September 2011

AbstractMetformin is the oral drug of first choice in type 2 diabetes. Therefore a large number of patients undergoing bariatric surgery will be on Metformin treatment. However, use of Metformin has been associated with lactate acidosis. Weight loss following bariatric surgery is most pronounced during the first weeks after the operation and this creates a phase of negative energy balance with ketone body formation. To shed more light on this situation we measured ketone bodies in 90 patients 5 days–18 months after bariatric surgery. Ketone bodies were markedly elevated during the first 3–4 months. Metformin use should therefore be critically reconsidered after bariatric operations.
Bilingual mathematics: development and practice in Wales
Authors: Jones Dylan V. 
Published: September 2011

AbstractThe first part of this paper provides a brief historical account of the development of mathematics teaching and learning through the medium of Welsh. It provides a review of developments which have seen the teaching and learning of mathematics through a native but minority language become an accepted part of the curriculum for a great number of pupils. It goes on to examine the nature of bilingual education currently to be found in Wales by considering two main types of bilingual schools which currently serve Welsh pupils and which have contributed towards a modest but notable minority language revival. It is in this context that current practice relating to Welsh‐medium mathematics is explored and a number of questions concerning current mathematics teaching, learning and assessment practices are raised. While celebrating the success and expansion of bilingual education in Wales, attention is drawn to the need to subject current practice to closer scrutiny. Although the paper focuses on the particular situation to be found in Wales, the issues and questions raised are clearly of interest to those involved with other bilingual educational programmes.
‘Challenge and support’: the role of the critical friend in continuing professional development
Authors: Holden Gary 
Published: September 2011

AbstractIn this paper, the writer, a senior teacher in a comprehensive school, describes his role as a critical friend to two teachers who were undertaking self‐initiated action research projects as part of a school‐based, award‐bearing curriculum development scheme offered in conjunction with the local higher education institute (HEI). He concludes that such a relationship, in providing both challenge and support for colleagues who are attempting to improve practice, is a powerful vehicle for the professional development of teachers and has a positive impact on whole‐school development.
First Report of Xanthomonas hortorum pv. hederae Causing Bacterial Leaf Spot of Hedera hibernica in Slovenia
Authors: Pirc M. , Dreo T. , Šuštaršič M. , Erjavec J. , Ravnikar M. 
Published: September 2011

In October 2008, water-soaked spots, each 5 to 10 mm in diameter and surrounded by a pale green halo, were observed on leaves of a single Atlantic ivy plant (Hedera hibernica Kirchn.) out of 89 plants imported from the European Union into a commercial greenhouse in Slovenia. Leaves were surface disinfected, and spots were cut and suspended in 10 mM phosphate buffered saline. From this extract, yellow-pigmented, Xanthomonas-like bacterial colonies were isolated onto nutrient agar, and two colonies (NIB Z 1310 and NIB Z 1312) were each identified as a Xanthomonas sp. based on biochemical tests (oxidase negative; positive for hydrolysis of H2S, starch, and tributyrin; and positive for acid production from sucrose). Both isolates caused a hypersensitive reaction (1) on leaves of tomato cv. Moneymaker. A repetitive extragenic palindromic sequence (REP)-PCR assay using the BOXA1R primer (3) resulted in highly similar DNA fragment banding patterns (Pearson's correlation: 95% identity) between the two isolates (NIB Z 1310 and NIB Z 1312) and the type strain of Xanthomonas hortorum pv. hederae CFBP 4925 (ICMP 453). Partial sequences of the gyrB gene (DNA gyrase, subunit B) (2) from isolates NIB Z 1310 (Accession No. JF794785; 599 bp) and NIB Z 1312 (Accession No. JF794784; 544 bp), showed identical sequences (100% identity with 100% coverage) to type strain ICMP 453 (Accession No. EU498975.1). The pathogenicity of the two isolates from H. hibernica was confirmed on three plants of H. helix ‘Evita’ (each 6 months old) for each isolate. Plants were sprayed on the abaxial and adaxial side of leaves with 10 ml of a 48-h suspension of the appropriate isolate with approximately 106 CFU/ml (1), covered individually with plastic bags for 24 h, and incubated under high relative humidity (>80%) with 16 h of daylight at 25°C by day and 20°C by night. Three positive and three negative control plants were inoculated with the type strain of X. hortorum pv. hederae CFBP 4925 and 0.01 M magnesium sulfate buffer, respectively. After 21 days, water-soaked spots with a pale green halo were observed on all plants inoculated with the bacterial strains, including the positive control plants. Colonies isolated from these lesions were identical in morphology and BOX-PCR DNA fragment banding patterns to the original isolates. Negative control plants did not develop symptoms, and colonies similar to X. hortorum pv. hederae were not isolated from these plants. To our knowledge, this is the first report of X. hortorum pv. hederae on H. hibernica in greenhouse production in Slovenia. If the disease spreads, it could reduce quality and marketability of this popular ground cover plant. References: (1) Z. Klement et al. Inoculation of Plant Tissues. In: Methods in Phytobacteriology. Akadémiai Kiadó, Budapest, 1990. (2) N. Parkinson et al. Int. J. Syst. Evol. Microbiol. 59:264, 2009. (3) J. Versalovic et al. Methods Mol. Cell Biol. 5:25, 1994.
Function and Evolution of a MicroRNA That Regulates a Ca2+-ATPase and Triggers the Formation of Phased Small Interfering RNAs in Tomato Reproductive Growth
Authors: Wang Ying , Itaya Asuka , Zhong Xuehua , Wu Yang , Zhang Jianfeng , van der Knaap Esther , Olmstead Richard , Qi Yijun , Ding Biao 
Published: September 2011

Abstract
MicroRNAs (miRNAs) regulate a wide variety of biological processes in most eukaryotes. We investigated the function and evolution of miR4376 in the family Solanaceae. We report that the 22-nucleotide miR4376 regulates the expression of an autoinhibited Ca2+-ATPase, tomato (Solanum lycopersicum) ACA10, which plays a critical role in tomato reproductive growth. Deep phylogenetic mapping suggested (1) an evolution course of MIR4376 loci and posttranscriptional processing of pre-miR4376 as a likely limiting step for the evolution of miR4376, (2) an independent phylogenetic origin of the miR4376 target site in ACA10 homologs, and (3) alternative splicing as a possible mechanism of eliminating such a target in some ACA10 homologs. Furthermore, miR4376 triggers the formation of phased small interfering RNAs (siRNAs) from Sl ACA10 and its Solanum tuberosum homolog. Together, our data provide experimental evidence of miRNA-regulated expression of universally important Ca2+-ATPases. The miR4376-regulated expression of ACA10 itself, and possibly also the associated formation of phased siRNAs, may function as a novel layer of molecular mechanisms underlying tomato reproductive growth. Finally, our data suggest that the stochastic emergence of a miRNA-target gene combination involves multiple molecular events at the genomic, transcriptional, and posttranscriptional levels that may vary drastically in even closely related species.
Anti‐helminth‐induced changes in the prevalence of entodiniomorphid ciliates in semi‐captive chimpanzees
Authors: Vincent Obanda , Stephen Chege , Patrick Chiyo 
Published: September 2011

AbstractCiliates are some of the most abundant gut fauna in wild chimpanzees. However, their presence in captive populations is usually low presumably due to anti‐helmintic prophylaxis or feeding on low fibre diet. We studied a semi‐captive colony of chimpanzees at the Sweetwaters Sanctuary in Kenya subject to routine prophylactic dose of albendazole to clear chimpanzees of parasitic helminths. Fresh faecal samples from known individuals were collected before and subsequently after prophylaxis. The samples were fixed in 10% formalin and examined by the sedimentation method. Troglodytella abrassarti had 42.5% prevalence whereas other ciliates had 65% prevalence. The prevalence of the T. abrassarti and other ciliates significantly declined immediately after prophylaxis and then rose slowly thereafter. Our results suggest that ciliates are susceptible to anti‐helmintic prophylaxis and that treatment may eliminate ciliates or inhibit their proliferation within the host subsequently lowering their prevalence in the population. Variation in prevalence was not influenced by the age of the host. However, a steady recovery of ciliate prevalence was lower for male compared to female hosts. Our results imply that the intervals between prophylactic regimes could be prolonged differently for males and females to increase the prevalence of ciliates in captive populations.
Barriers to collaboration on behalf of children with challenging behaviours: a large qualitative study of five constituent groups
Authors: McLean Sara 
Published: September 2011

ABSTRACTInter‐agency collaboration is seen as an effective way to support children and families with multiple and complex needs. Children in out‐of‐home care, especially those exhibiting challenging and disruptive behaviour, often require the services of multiple agencies. Ninety‐two South Australian stakeholders experienced in supporting children in out‐of‐home care were interviewed about their experience of collaborative practice, using the specific example of supporting children with extremely challenging behaviour. Participants were teachers, foster parents, child welfare workers, child mental health professionals and residential care workers. Thematic analysis revealed several tensions inherent in such collaboration. Results are discussed in terms of their unique implications for managing challenging behaviour, and suggestions are made for improving collaborative approaches to behaviour and multi‐agency practice.
Ant body posture: gaster curling increases ant speed
Authors: AMADOR‐VARGAS SABRINA , MARTÍNEZ JUAN ANDRÉS , GIRALDO‐BELTRÁN PAOLA , GONZÁLEZ ROSA MARÍA , RIFKIN SETH , GAMARRA‐TOLEDO VICTOR 
Published: September 2011

1. Body postures adopted by an animal can serve behavioural functions, homeostasis, or energy balance.2. We investigated the function of holding the gaster curled forward under the thorax in acacia ants, Pseudomyrmex spinicola Emery, by testing whether ants adopted this posture for defence, thermoregulation, or for efficient locomotion.3. For the defence hypothesis, we expected an increase in the proportion of ants with curled gasters after a visual threat, a vibrational disturbance of a branch, or the release of nestmate's alarm pheromones. Our data did not support these predictions.4. For the thermoregulation hypothesis, we found a positive correlation between temperature and proportion of curled‐gaster ants. However, we did not find a reduction in the proportion of curled‐gaster ants after shading them, as predicted by this hypothesis.5. Our data supported the locomotion hypothesis: curled‐gaster ants walked 1 cm s−1 faster than ants with the gaster held straight. Straight‐gaster ants walked with the thorax closer to the surface, a posture that likely shifts the centre of gravity closer to the surface in a manner similar to gaster curling.6. Studying the role of the body posture in acacia ants and other insects will provide a better understanding of the kinematics of walking in challenging angles with respect to gravity.
Normalized downward continuation of potential fields within the quasi‐harmonic region
Authors: Fedi Maurizio , Florio Giovanni 
Published: September 2011

ABSTRACTDownward continuation is a useful transformation, mainly used to enhance measured gravity or magnetic field anomalies. It is known to be an unstable transformation that should be strictly used only in the harmonic region, apparently preventing any meaningful application to continuations inside the source volume. Despite these well‐known theoretical and practical limitations it has been used to recover source parameters by different methods, here referred to as normalized full gradient methods. Such methods show that downward continuation may be extended to the source volume, which is assumed to contain one‐point, isolated singularities, which is a quasi‐harmonic region. We modify the normalized full gradient method focusing our attention to the way the downward continuation is normalized. Differently from normalized full gradient methods, we study the effect of the normalization not only on the analytical signal modulus of the downward continued field but also on the downward continuation of the gravity or magnetic fields themselves. With our method, called normalized downward continuation, several statistically meaningful normalizations are considered, some of them yielding improved, more resolved depth estimations for synthetic as well as measured total‐field anomalies. From a statistical point of view, the downward continued field tends to have right‐skewed histograms at shallow depths, while becoming symmetrically distributed at greater depths. This occurs because, as the depth of continuation increases, the intrinsic error propagation of the downward continuation allows the error to dominate with respect to the source‐related signal. For non‐isolated anomalies, consistent results are also obtained but the normalizing factors must be computed within windows centred to the studied anomaly.
Interesterification of milkfat with soybean oil catalysed by Rhizopus oryzae lipase immobilised on SiO2‐PVA on packed bed reactor
Authors: Paula Ariela V. , Nunes Gisele F. M. , Santos Júlio C. , de Castro Heizir F. 
Published: September 2011

SummaryThe potential of the lipase from Rhizopus oryzae immobilised on SiO2‐PVA to catalyse the interesterification of the milkfat with soybean oil in a packed bed reactor running on continuous mode was evaluated. The reactor operated continuously for 35 days at 45 °C, and during 12 days, no significant decrease in the initial lipase activity was verified. Interesterification yields were in the range from 35 to 38%wt, which gave an interesterified product having 59% lower consistency in relation to non‐interesterified blend. Results showed the potential of the lipase from Rhizopus oryzae to mediate the interesterification of milkfat with soybean oil in packed bed reactor, attaining a more spreadable product under a cool temperature. The biocatalyst operational stability was assessed and an inactivation profile was found to follow the Arrhenius model, revealing values of 34 days and 0.034 day−1, for half‐life and a deactivation coefficient, respectively.
Informal risk assessment strategies in health care staff: an unrecognized source of resilience?
Authors: Arfanis Konstantinos , Smith Andrew 
Published: September 2011

AbstractRationale  Recent reports indicate that approximately 10% of in‐patients in UK hospitals are involved in an adverse event (these reports also state that 50% of these events are preventable). This is indeed a worrying finding, and indicates the need to look at how these incidents are handled or indeed, what is done to minimize their occurrence. The Department of Health, via the National Patient Safety Agency (NPSA) published a guide which is aimed at encouraging accurate reporting, learning from past events and changing the attitudes of key stakeholders (healthcare managers, frontline staf etc) towards risk taking and risk management.Aims and objectives  Our aim was to compare informally‐learned and used risk assessment strategies volunteered by staff with the ‘how to do it’ guide published by the NPSA. We have compared each step of the NPSA guide with our empirical data relating to that activity.Methods  We interviewed forty‐eight healthcare professionals (doctors from several specialties; nurses from a variety of settings; and an array of allied healthcare professionals). We used semi‐structured interviews in order to discuss participants' views on their everyday working life, working relationships and patient safety.Results  Our results indicate that healthcare professionals develop their own unique way to approach the issue of patient safety and risk, based on their professional raining, seniority and role within the hospital. They did share the conviction that frontline and support staff need to have immediate and easy access to information about past adverse events. They see this as a powerful tool in minimizing the reoccurrence of the same errors/problems, as well as a vehicle to improve staff morale by feeling valued and having their opinion heard.Conclusions  We believe that patient safety and welfare can benefit from the adoption of a more flexible and person‐centred approach to how risk assessments are carried out. Enriching formal guidelines such as the ‘Risk Assessment made easy’ document with successful techniques and strategies which healthcare staff have informally developed has the potential to not only improve patient safety (since it will be based on the accumulated experience and knowledge of such staff) but also foster higher levels of self esteem amongst healthcare professionals.