bluuebunny/crossref_metadata_2025_split huggingface dataset:
Combined application of artificial bone powders and concentrated growth factor membranes on the autotransplantation of mature third molars: A 5‐year retrospective case series
Authors: Ainiwaer Ailimaierdan , Tuerdi Maimaitituxun , Zuolipahaer Zulikamaier , Wang Ling 
Published: June 2024

AbstractAimThis study aimed to observe the efficacy and outcomes of the combined application of artificial bone powder and concentrated growth factor (CGF) membranes for tooth transplantation in cases with wide recipient sockets and small donor teeth.Material and MethodsA total of 36 teeth from 36 patients with wide recipient sockets and small donor teeth were enrolled. Autogenous tooth transplantation was performed using bone powders and CGF membranes. After transplantation, the visual analog scale (VAS) score, Landry Wound Healing Index, probing depth (PD), mobility, and gray value of the alveolar bone around the transplanted teeth were measured, and a patient satisfaction questionnaire was administered. All patients underwent clinical and radiographic examinations during follow‐up.ResultsThe VAS score of 16 (44.4%) cases after 1 week was 0, and 26 (72.2%) patients showed excellent gingival healing after 2–4 weeks. The PD of a few cases was deeper than 3 mm during the first month; however, returned to normal after 3 months. Although the majority of the transplanted teeth possessed mobility greater than grade I during the first month, the mobility gradually improved within 3 months. The gray value of the alveolar bone around the transplanted teeth, 1 year postoperatively showed no difference with pre‐operation (p > .05). During the mean follow‐up period of 42.7 months (range 20–72 months), 33 of the 36 transplanted teeth remained in situ without clinical or radiographic complications, with an overall success rate of 91.7%.ConclusionsAlthough the PD and mobility of the transplanted teeth were not ideal during the early stages of healing, most of the transplanted teeth had good clinical outcomes. In cases with large recipient sites accompanied by small donor teeth, autotransplantation of teeth using artificial bone powder combined with CGF membranes is a viable option and can lead to optimistic results with favorable success rates.
Effects of azoxystrobin and difenoconazole on the development, fecundity, and predatory capacity of Cyrtorhinus lividipennis
Authors: Hu Kui , Cheng Shichang , Chen Ke , Qiu Lin , Liu Fang 
Published: June 2024

AbstractThe predatory bug Cyrtorhinus lividipennis Reuter (Hemiptera: Miridae) is an important biological control agent for planthoppers, such as Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), and leafhoppers in rice fields (Oryza sativa L., Poaceae). In this study, the sublethal effects of azoxystrobin and difenoconazole, two fungicides extensively used in rice production, on this predatory bug were investigated. The results indicated that exposure to the two fungicides at the third instar had no influence on developmental duration, but difenoconazole resulted in a 25.2% decrease in the lifespan of adult males. When exposed as newly emerged adults, azoxystrobin caused a 33.5% decrease in female longevity. The pre‐oviposition period of azoxystrobin‐treated females mating with azoxystrobin‐treated males was shortened by 44.9%, compared with the control, whereas the pre‐oviposition period of difenoconazole‐treated females mating with control males was lengthened by 32.2% compared with the control group. However, the two fungicides had no effect on fecundity and egg hatchability. Furthermore, the two fungicides did not impact the average consumption of C. lividipennis of N. lugens individuals. They did stimulate the response of C. lividipennis males to rice plants infested by N. lugens in a two‐choice H‐shaped olfactometer test. Also, the predatory capacity of C. lividipennis males was increased after exposure to difenoconazole, whereas the predatory capacity of females was decreased after exposure to the two fungicides. Overall, our results indicated that azoxystrobin and difenoconazole pose no notable risk to C. lividipennis. These findings establish a foundation for the rational and coordinated utilization of fungicides and biological control agents in rice ecosystems.
The expression of Galectin‐9 correlates with mTOR and AMPK in murine colony‐forming erythroid progenitors
Authors: Tsukamoto Tetsuo 
Published: June 2024

AbstractObjectivesGalectin‐9 (Gal‐9) is an immune checkpoint ligand for T‐cell immunoglobulin and mucin domain 3. Although the roles of Gal‐9 in regulating immune responses have been well investigated, their biological roles have yet to be fully documented. This study aimed to analyse the expression of Gal‐9 bone marrow (BM) cells in C57BL/6J (B6) mice. Furthermore, the co‐expression of Gal‐9 with the mammalian target of rapamycin (mTOR) and AMP‐activated protein kinase (AMPK) was investigated.MethodsThe BM cells in adult C57BL/6J (B6) mice were collected and analysed in vitro.ResultsIn a flow cytometric analysis of BM cells, Gal‐9 was highly expressed in c‐KithiSca‐1−CD34−CD71+ erythroid progenitors (EPs), whereas it was downregulated in more differentiated c‐KitloCD71+TER119+ cells. Subsequently, a negative selection of CD3−B220−Sca‐1−CD34−CD41−CD16/32− EPs was performed. This resulted in substantial enrichment of KithiCD71+Gal‐9+ cells and erythroid colony‐forming units (CFU‐Es), suggesting that the colony‐forming subset of EPs are included in the KithiCD71+Gal‐9+ population. Furthermore, we found that EPs had lower mTOR and AMPK expression levels in Gal‐9 knockout B6 mice than in wild‐type B6 mice.ConclusionsThese results may stimulate further investigation of the role of Gal‐9 in haematopoiesis.
Frequency‐dependent tolerance to aircraft disturbance drastically alters predicted impact on shorebirds
Authors: van der Kolk Henk‐Jan , Smit Cor J. , Allen Andrew M. , Ens Bruno J. , van de Pol Martijn 
Published: June 2024

AbstractAnthropogenic disturbance of wildlife is increasing globally. Generalizing impacts of disturbance to novel situations is challenging, as the tolerance of animals to human activities varies with disturbance frequency (e.g. due to habituation). Few studies have quantified frequency‐dependent tolerance, let alone determined how it affects predictions of disturbance impacts when these are extrapolated over large areas. In a comparative study across a gradient of air traffic intensities, we show that birds nearly always fled (80%) if aircraft were rare, while birds rarely responded (7%) if traffic was frequent. When extrapolating site‐specific responses to an entire region, accounting for frequency‐dependent tolerance dramatically alters the predicted costs of disturbance: the disturbance map homogenizes with fewer hotspots. Quantifying frequency‐dependent tolerance has proven challenging, but we propose that (i) ignoring it causes extrapolations of disturbance impacts from single sites to be unreliable, and (ii) it can reconcile published idiosyncratic species‐ or source‐specific disturbance responses.
Association of APOE genotype and cerebrospinal fluid Aβ and tau biomarkers with cognitive and motor phenotype in amyotrophic lateral sclerosis
Authors: Maranzano Alessio , Verde Federico , Dubini Antonella , Torre Silvia , Colombo Eleonora , Doretti Alberto , Gentile Francesco , Manini Arianna , Milone Ilaria , Brusati Alberto , Peverelli Silvia , Santangelo Serena , Spinelli Edoardo Gioele , Torresani Erminio , Gentilini Davide , Messina Stefano , Morelli Claudia , Poletti Barbara , Agosta Federica , Ratti Antonia , Filippi Massimo , Silani Vincenzo , Ticozzi Nicola 
Published: June 2024

AbstractObjectiveLittle is known about amyotrophic lateral sclerosis (ALS)‐nonspecific cognitive deficits – most notably memory disturbance – and their biological underpinnings. We investigated the associations of the Alzheimer's disease (AD) genetic risk factor APOE and cerebrospinal fluid (CSF) biomarkers Aβ and tau proteins with cognitive and motor phenotype in ALS.MethodsAPOE haplotype was determined in 281 ALS patients; for 105 of these, CSF levels of Aβ42, Aβ40, total tau (T‐tau), and phosphorylated tau (P‐tau181) were quantified by chemiluminescence enzyme immunoassay (CLEIA). The Edinburgh Cognitive and Behavioural ALS Screen (ECAS) was employed to evaluate the neuropsychological phenotype.ResultsAPOE‐E4 allele was associated with worse ECAS memory score (median, 14.0 in carriers vs. 16.0 in non‐carriers) and lower CSF Aβ42 (−0.8 vs. 0.1, log‐transformed values) and Aβ42/40 ratio (−0.1 vs. 0.3). Some 37.1% of ALS patients showed low Aβ42 levels, possibly reflecting cerebral Aβ deposition. While lower Aβ42/40 correlated with lower memory score (β = 0.20), Aβ42 positively correlated with both ALS‐specific (β = 0.24) and ALS‐nonspecific (β = 0.24) scores. Although Aβ42/40 negatively correlated with T‐tau (β = −0.29) and P‐tau181 (β = −0.33), we found an unexpected positive association of Aβ42 and Aβ40 with both tau proteins. Regarding motor phenotype, lower levels of Aβ species were associated with lower motor neuron (LMN) signs (Aβ40: β = 0.34; Aβ42: β = 0.22).ConclusionsAPOE haplotype and CSF Aβ biomarkers are associated with cognitive deficits in ALS and particularly with memory impairment. This might partly reflect AD‐like pathophysiological processes, but additional ALS‐specific mechanisms could be involved.
Multi‐model deep learning system for screening human monkeypox using skin images
Authors: Gupta Kapil , Bajaj Varun , Jain Deepak Kumar , Hussain Amir 
Published: June 2024

AbstractPurposeHuman monkeypox (MPX) is a viral infection that transmits between individuals via direct contact with animals, bodily fluids, respiratory droplets, and contaminated objects like bedding. Traditional manual screening for the MPX infection is a time‐consuming process prone to human error. Therefore, a computer‐aided MPX screening approach utilizing skin lesion images to enhance clinical performance and alleviate the workload of healthcare providers is needed. The primary objective of this work is to devise an expert system that accurately classifies MPX images for the automatic detection of MPX subjects.MethodsThis work presents a multi‐modal deep learning system through the fusion of convolutional neural network (CNN) and machine learning algorithms, which effectively and autonomously detect MPX‐infected subjects using skin lesion images. The proposed framework, termed MPXCN‐Net is developed by fusing deep features of three pre‐trained CNNs: MobileNetV2, DarkNet19, and ResNet18. Three classifiers—K‐nearest neighbour, support vector machine (SVM), and ensemble classifier—with various kernel functions, are used to identify infected patients. To validate the efficacy of our proposed system, we employ a publicly accessible MPX skin lesion dataset.ResultsBy amalgamating features extracted from all three CNNs and utilizing the medium Gaussian kernel of the SVM classifier, our proposed system achieves an outstanding average classification accuracy of 90.4%.ConclusionsDeveloped MPXCN‐Net is suitable for testing with a large diversified dataset before being used in clinical settings.
Among‐river pattern in relative abundance of two salmonid fishes reflects temperature‐dependent competition
Authors: Skoglund Helge , Forseth Torbjørn , Einum Sigurd 
Published: June 2024

Abstract

Temperature is a key driver in determining species distribution and abundance across climatic and geographical gradients. This occurs through direct physiological effects of temperature on performance, but may also result from temperature effects on species interactions.

In the current study we experimentally test for temperature‐dependent competition between juvenile anadromous Atlantic salmon and brown trout and evaluate the role of temperature in shaping the relative abundance of these species across 88 Norwegian rivers.

When the two species were reared in allopatry their somatic growth rates were similar across a range of temperatures (4–16°C).

When reared in sympatry, the growth of salmon was greatly reduced at the coldest temperature relative to when reared in allopatry, whereas this was not the case for trout. The effect of interspecific competition on growth was more similar for the two species at warmer temperatures. Thus, interspecific competition effects were strongly asymmetric only at the lowest temperature, with trout outcompeting salmon.

The results from the experiment were reflected in qualitative patterns of relative abundance of salmon and trout in natural populations, which shifted from being trout dominated in rivers with low summer temperatures to being salmon dominated in warmer rivers.

These results provide an empirical example of how temperature effects on the relative abundance of species can only be understood in light of information about temperature‐dependent species interactions.
Seasonal Groundwater Level Dynamics in Unconfined Aquifers across the United States
Authors: Stahl Mason O. , Mar Tyler , Jameel Yusuf 
Published: June 2024

AbstractGroundwater hydrographs contain a rich set of information on the dynamics of aquifer systems and the processes and properties that influence them. While the importance of seasonal cycles in hydrologic and environmental state variables is widely recognized there has yet to be a comprehensive analysis of the seasonal dynamics of groundwater across the United States. Here we use time series of groundwater level measurements from 997 wells from the National Groundwater Monitoring Network to identify and describe groundwater seasonal cycles in unconfined aquifers across the United States. We use functional data analysis to obtain a functional form fit for each site and apply an unsupervised clustering algorithm to identify a set of five distinct seasonal cycles regimes. Each seasonal cycle regime has a distinctive shape and distinct timing of its annual minimum and maximum water level. There are clear spatial patterns in the occurrence of each seasonal cycle regime, with the relative occurrence of each regime strongly influenced by the geologic setting (aquifer system), climate, and topography. Our findings provide a comprehensive characterization of groundwater seasonal cycles across much of the United States and present both a methodology and results useful for assessing and understanding unconfined groundwater systems.
The Perspectives of Healthcare Professionals and Managers on Patient Involvement in Care Pathway Development: A Discourse Analysis
Authors: Visser Mildred , ‘t Hart Naomi , de Mul Marleen , Weggelaar‐Jansen Anne Marie 
Published: June 2024

ABSTRACTBackgroundThe WHO advocates patient and public involvement as an ethical imperative, due to the value of the lived experience of patients. A deeper understanding of the shared meanings and underlying beliefs of healthcare professionals and managers for and against including patients in care pathway development.ObjectiveTo explore the considerations of healthcare professionals and managers on the involvement of patients and public in care pathway development.MethodsIn a medical rehabilitation centre we conducted a single case study that was part of a 2‐year action research programme on blended care pathway development. Following 14 semistructured interviews with healthcare professionals and managers, we analysed their discourses on the value of patient involvement as well as the potential threats and opportunities.ResultsWe identified four discourses. Patient as expert frames involvement as relevant, as adding new perspectives and as required to fully understand the patient's needs. Skills and representation is based on the construct that obtaining valuable insights from patients requires certain skills and competences. Self‐protection focusses on personal, interprofessional objections to patient involvement. Professional knows best reveals expertise‐related reasons for avoiding or postponing involvement.ConclusionThese discourses explain why patient and public involvement in care pathway development is sometimes postponed, limited in scope and level of participation, and/or avoided. The following strategies might minimise the paralysing effect of these discourses: strengthen the capabilities of all stakeholders involved; use a mix of complementary techniques to gain involvement in distinct phases of care pathway development; and create/facilitate a safe environment. Put together, these strategies would foster ongoing, reciprocal learning that could enhance patient involvement.Patient or Public ContributionThis study belonged to an action research programme on blended care pathway development (developing an integrated, coordinated patient care plan that combines remote, digital telehealth applications, self‐management tools and face‐to‐face care). Multidisciplinary teams took a quality collaborative approach to quality improvement (considering patients as stakeholders) to develop 11 blended care pathways. Although professionals and managers were instructed to invite patients onto their teams and to attend care pathway design workshops, few teams (3/11) actually did. Unravelling why this happened will help improve patient and public involvement in care pathway development.
Variations in soybean nutritional and anti‐nutritional quality based on location and planting dates
Authors: Alfaro‐Wisaquillo Maria Camila , Ali Muhammad , Patiño Danny , Oviedo‐Rondon Edgar Orlando , Vann Rachel , Joseph Michael 
Published: June 2024

SummarySoybeans (Glycine max) are a preferred source of plant‐based proteins and oil in various applications in foods and feeds. When soybean is converted to soybean meal (SBM), the quality is determined by the protein content and its digestibility. Protein digestibility is reduced by anti‐nutritional factors like trypsin inhibitors (TI) and processing. The abundant genetic variations in soybean germplasm affect the quantitative trait of protein content. This study analysed the effect of location and planting dates on genetically identical soybeans grown in North Carolina (NC) and then compared the nutritional traits to similar commonly grown soybean varieties in other locations in the USA. The soybeans were analysed using NIRS and wet chemistry. It was found that soybean protein content ranged from 38.14% to 44.92% among the different varieties grown in three different locations in NC. Similarly, TI levels ranged between 28.22 and 33.11 mg g−1 among these varieties. The same varieties, when planted as either a full season or double crop, had different TI levels and were also impacted by planting location. However, there were no significant differences in the TI levels between the varieties and planting seasons. Furthermore, no significant difference in protein content among the varieties within the planting dates was found. Compared to soybeans grown in other parts of the USA, the average crude protein (CP) content was similar to those grown in Arkansas and Minnesota. The TI levels were significantly higher than those grown in Ohio, similar to that of Arkansas, and significantly lower than that of Minnesota.
The content and distribution pattern of chemicals and bioactive compounds of triticale grains
Authors: Jiang Zefang , Li Mo , Han Qianyun , Zhou Shiyue , Wen Xin , Ni Yuanying 
Published: June 2024

SummaryThere is accumulating evidence that triticale‐based foods have potential health benefits. Herein, we milled triticale grains into five components (break flour, reduction flour, coarse bran, fine bran and whole flour) and analysed their nutritional profiles. Our findings revealed that triticale flour has higher content of minerals (2.04 g 100 g−1), insoluble dietary fibre (14.58 g 100 g−1), polyunsaturated fatty acids (1055.3 μg g−1) and vitamin B9 (0.031 mg 100 g−1) and lower levels of lipids and total essential amino acids than wheat flour. All milling fractions showed higher amounts of unsaturated than saturated fatty acids. Coarse bran and fine bran had the highest mineral and vitamin B content. Break flour has significant amounts of total and essential amino acids, whereas reduction flour has significant amounts of starch and amylose. Break and reduction flour have high mineral bioaccessibility. Thus, milling fractions have unique nutritional properties, which is important for the comprehensive utilisation of triticale.
Exploring the properties of Garcinia indica Linn rind powder and its possible usage in food industry
Authors: Awari Ankita , Kumar Mukul , Kaushik Deepika , Oz Emel , Proestos Charalampos , Zeng Maomao , Brennan Charles , Ahmed Mukhtar , Oz Fatih 
Published: June 2024

SummaryThe study investigates, the potential utilisation of the Garcinia indica linn rind powder as a valuable and healthy ingredient for food industry. Techno‐functional properties revealed the functional attributes which is crucial for product development. Characterisation of the powder and extract revealed the physical, chemical, and structural properties through FTIR, HPLC, and SEM. Therefore, moisture content (5.45%), ash content (2.80%), fibre content (7.56%), and fat content (9.54%) were reported in G. indica linn rind. Phytochemical analysis indicated significant levels of phenolic and flavonoid compounds, suggesting antioxidant potential, supported by inhibition in the DPPH assay with IC50 value of 485.1 μg/mL. In‐vitro assays revealed inhibitory effects on α‐amylase with IC50 value of 58.43 μg/mL and glucose with an IC50 value of 0.39 μg/mL, indicating potential benefits in blood glucose management and weight control. Overall, G. indica rind powder shows promise as a natural ingredient with diverse applications in functional foods and nutraceuticals, offering potential health benefits through innovative product formulations.
Profiles of Campylobacter jejuni from raw retail chicken meat: genetic diversity, pathogenic features, and antibiotic resistance
Authors: Hizlisoy Harun , Barel Mukaddes , Dishan Adalet , Al Serhat , Gungor Candan , Koskeroglu Kursat , Disli H. Burak , Hizlisoy Serhat , Onmaz Nurhan Ertas , Yildirim Yeliz , Gonulalan Zafer , Gumussoy K. Semih 
Published: June 2024

SummaryThe study aimed to assess Campylobacter jejuni prevalence in chicken meat, biofilm formation, virulence factors, antibiotic resistance, and molecular typing. In the study, 200 chicken meat samples were collected from local outlets and 51 (25.5%) isolates were identified as C. jejuni. Resistance rates to ampicillin, tetracycline, sulfamethoxazole/trimethoprim, and ciprofloxacin were 59%, 60%, 64%, and 64% respectively. Many of the isolates (49%) exhibited multidrug resistance. Beta‐lactamase and tetracycline resistance genes were found in 82.3% and 86.2% of isolates, respectively. Virulence genes were detected in various proportions. Biofilm formation was weak to moderate. ERIC‐PCR showed varied band profiles. Whole genome sequencing confirmed findings. The study highlights C. jejuni presence with antibiotic resistance, virulence and biofilm features in chicken meat, posing public health risks.