bluuebunny/crossref_metadata_2025_split huggingface dataset:
Perceived health status after mid‐urethral sling revision in 287 women from the VIGI‐MESH registry: A cross‐sectional study
Authors: Camilli Hugo , Fatton Brigitte , Gand Elise , Campagne‐Loiseau Sandrine , De Tayrac Renaud , Wagner Laurent , Saussine Christian , Rigaud Jérôme , Thubert Thibault , Deffieux Xavier , Cosson Michel , Ferry Philippe , Capon Grégoire , Panel Laure , Chartier‐Kastler Emmanuel , Gauthier Tristan , Game Xavier , Bouynat Cassandra , Bichon Romane , Fauconnier Arnaud , Pizzoferrato Anne‐Cécile , Fritel Xavier 
Published: May 2024

AbstractObjectiveTo evaluate the health status and recovery of women after mid‐urethral sling (MUS) revision in response to complications.DesignCross‐sectional study using a questionnaire sent to women from a registry.SettingTwenty‐two French surgical centres.PopulationA total of 287 women from the VIGI‐MESH registry responded, having undergone MUS revision for complications.MethodsOur sample of women were compared against a representative set of French women taken from the Eurostat database. Multivariate analysis was performed to identify clinical predictors for successful MUS revision. A qualitative analysis was carried out on free‐text comments.Main outcome measuresHealth status, defined by the Minimum European Health Module, and recovery, assessed by Patient Global Impression of Improvement.ResultsThe response rate was 76% (287/378), with 49% of the women (141/287, 95% CI 43%–55%) reporting good health status, which was 8 points lower than that expected from the comparator French set (57%, 95% CI 55%–58%). Overall, 53% (147/275, 95% CI 47%–59%) of the women reported feeling much better after MUS revision. Just over one‐third (35%, 95/275, 95% CI 29%–40%) of respondents reported poor health with little or no improvement. Multivariate analysis showed that being operated on for pain at revision was associated with worse self‐perceived health than being operated on for exposure (OR 0.36, 95% CI 0.14–0.95); women with pre‐existing comorbidity reported a poorer health status following MUS revision (OR 0.22, 95% CI 0.13–0.38).ConclusionsOur results suggest that half of the women recovered good health status after MUS revision, whereas a proportion appeared to be seriously affected by an MUS complication despite the revision.
Nondestructive testing of runny salted egg yolk based on improved ConvNeXt‐T
Authors: Chen Haoran , Chang Yulong , Chen Yuanzhe , Liu ChenKang , Zhu ZhiHui , Liu ShiWei , Wang Qiaohua 
Published: May 2024

AbstractSalted egg yolks from salted duck eggs are widely utilized in the domestic and international food industry as both raw materials and ingredients. When salted egg yolks are not fully cured and matured, they exist in a fluid state, with a mixture of solid and liquid internally. Due to this composition, they are susceptible to deterioration during storage and usage, necessitating their detection and classification. In this study, a dataset specifically for salted egg yolks was established, and the ConvNeXt‐T model, employed as the benchmark model, underwent two notable improvements. First, a lightweight location‐aware circular convolution (ParC) was introduced, utilizing a ParC‐block to replace a portion of the original ConvNeXt‐T block. This enhancement aimed to overcome the limitations of convolution in extracting global feature information while integrating the global sensing capability of vision transformer and the localization capability of convolution. Additionally, the activation function was modified through substitution. These improvements resulted in the final model. Experimental results indicate that the enhanced model exhibits faster convergence on the custom salted egg yolk dataset compared to the baseline model. Furthermore, a significant reduction of model parameters by a factor of 4 led to a 2.167 percentage point improvement in the accuracy of the test set. The ParC‐ConvNeXt‐SMU‐T model achieved an accuracy of 96.833% with 26.8 million parameters. Notably, the improved model demonstrates exceptional effectiveness in recognizing salted egg yolks.Practical ApplicationThis study can be widely applied in the process of salted egg yolk production and quality inspection, which can improve the actual sorting efficiency of salted egg yolks and reduce the labor cost at the same time. It can also be used for nondestructive testing of salted egg yolks by governmental enterprises and other regulatory authorities.
Effects of preheat treatment and syringic acid modification on the structure, functional properties, and stability of black soybean protein isolate
Authors: Miao Qianqian , He Yiqing , Sun Haiwen , Olajide Tosin Michael , Yang Minxin , Han Bingyao , Liao Xianyan , Huang Junyi 
Published: May 2024

AbstractThis study investigated preheated (25–100°C) black soybean protein isolate (BSPI) conjugated with syringic acid (SA) (25 and 50 µmol/g protein) under alkaline conditions, focusing on the structure, functional properties, and storage stability. The results revealed that the SA binding equivalent and binding rate on BSPI increased continuously as the preheat temperature increased. Additionally, preheating positively impacted the surface hydrophobicity (H0) of BSPI, with further enhancement observed upon SA binding. Preheating and SA binding altered the secondary and tertiary structure of BSPI, resulting in protein unfolding and increased molecular flexibility. The improvement in BSPI functional properties was closely associated with both preheating temperature and SA binding. Specifically, preheating decreased the solubility of BSPI but enhanced the emulsifying activity index (EAI) and foaming capacity (FC) of BSPI. Conversely, SA binding increased the solubility of BSPI with an accompanying increase in EAI, FC, foaming stability, and antioxidant activity. Notably, the BSPI100‐SA50 exhibited the most significant improvement in functional properties, particularly in solubility, emulsifying, and foaming attributes. Moreover, the BSPI‐SA conjugates demonstrated good stability of SA during storage, which positively correlated with the preheating temperature. This study proposes a novel BSPI‐SA conjugate with enhanced essential functional properties, underscoring the potential of preheated BSPI‐SA conjugates to improve SA storage stability.Practical ApplicationPreheated BSPI‐SA conjugates can be used as functional ingredients in food or health products. In addition, preheated BSPI shows potential as a candidate for encapsulating and delivering hydrophobic bioactive compounds.
A methyl esterase from Bifidobacterium longum subsp. longum reshapes the prebiotic properties of apple pectin by triggering differential modulatory capacity in faecal cultures
Authors: Calvete‐Torre Inés , Sabater Carlos , Muñoz‐Almagro Nerea , Campelo Ana Belén , Moreno F. Javier , Margolles Abelardo , Ruiz Lorena 
Published: May 2024

AbstractPectin structures have received increasing attention as emergent prebiotics due to their capacity to promote beneficial intestinal bacteria. Yet the collective activity of gut bacterial communities to cooperatively metabolize structural variants of this substrate remains largely unknown. Herein, the characterization of a pectin methylesterase, BpeM, from Bifidobacterium longum subsp. longum, is reported. The purified enzyme was able to remove methyl groups from highly methoxylated apple pectin, and the mathematical modelling of its activity enabled to tightly control the reaction conditions to achieve predefined final degrees of methyl‐esterification in the resultant pectin. Demethylated pectin, generated by BpeM, exhibited differential fermentation patterns by gut microbial communities in in vitro mixed faecal cultures, promoting a stronger increase of bacterial genera associated with beneficial effects including Lactobacillus, Bifidobacterium and Collinsella. Our findings demonstrate that controlled pectin demethylation by the action of a B. longum esterase selectively modifies its prebiotic fermentation pattern, producing substrates that promote targeted bacterial groups more efficiently. This opens new possibilities to exploit biotechnological applications of enzymes from gut commensals to programme prebiotic properties.
Listening to patients, for the patients: The COVAD Study—Vision, organizational structure, and challenges
Authors: Joshi Mrudula , Darooka Naitica , Saha Sreoshy , Dyball Sarah , Sen Parikshit , Yaadav Praggya , Javaid Mahnoor , Kadam Esha , Shinjo Samuel Katsuyuki , Dey Dzifa , Cavagna Lorenzo , Makol Ashima , Gutiérrez Carlos Enrique Toro , Caballero Uribe Carlo V. , Kuwana Masataka , Burmester Gerd‐Rüdiger , Ziade Nelly , Wincup Chris , Andreoli Laura , Parodis Ioannis , Tan Ai Lyn , Guillemin Francis , Knitza Johannes , Wang Guochun , Dalbeth Nicola , Velikova Tsvetelina , Gracia‐Ramos Abraham Edgar , Nikiphorou Elena , Day Jessica , Chinoy Hector , Aggarwal Rohit , Agarwal Vikas , Gupta Latika ,   the COVAD study group
Published: May 2024

AbstractBackgroundThe pandemic presented unique challenges for individuals with autoimmune and rheumatic diseases (AIRDs) due to their underlying condition, the effects of immunosuppressive treatments, and increased vaccine hesitancy.ObjectivesThe COVID‐19 vaccination in autoimmune diseases (COVAD) study, a series of ongoing, patient self‐reported surveys were conceived with the vision of being a unique tool to gather patient perspectives on AIRDs. It involved a multinational, multicenter collaborative effort amidst a global lockdown.MethodsLeveraging social media as a research tool, COVAD collected data using validated patient‐reported outcomes (PROs). The study, comprising a core team, steering committee, and global collaborators, facilitated data collection and analysis. A pilot‐tested, validated survey, featuring questions regarding COVID‐19 infection, vaccination and outcomes, patient demographics, and PROs was circulated to patients with AIRDs and healthy controls (HCs).DiscussionWe present the challenges encountered during this international collaborative project, including coordination, data management, funding constraints, language barriers, and authorship concerns, while highlighting the measures taken to address them.ConclusionCollaborative virtual models offer a dynamic new frontier in medical research and are vital to studying rare diseases. The COVAD study demonstrates the potential of online platforms for conducting large‐scale, patient‐focused research and underscores the importance of integrating patient perspective into clinical care. Care of patients is our central motivation, and it is essential to recognize their voices as equal stakeholders and valued partners in the study of the conditions that affect them.
A new Finnish flavor of feline coat coloration, “salmiak,” is associated with a 95‐kb deletion downstream of the KIT gene
Authors: Anderson Heidi , Salonen Milla , Toivola Sari , Blades Matthew , Lyons Leslie A. , Forman Oliver P. , Hytönen Marjo K. , Lohi Hannes 
Published: May 2024

AbstractCats with a distinctive white hair pattern of unknown molecular cause have been discovered in the Finnish domestic cat population. Based on the unique appearance of these cats, we have named this phenotype salmiak (“salty licorice”). The use of a commercially available panel test to genotype four salmiak‐colored cats revealed the absence of all known variants associated with white‐haired phenotypic loci: full White (W), Spotting (Ws) and the Birman white Gloves associated (wg) allele of the KIT proto‐oncogene (KIT) gene. Whole‐genome sequencing on two salmiak‐colored cats was conducted to search for candidate causal variants in the KIT gene. Despite a lack of coding variants, visual inspection of the short read alignments revealed a large ~95 kb deletion located ~65 kb downstream of the KIT gene in the salmiak cats. Additional PCR genotyping of 180 domestic cats and three salmiak‐colored cats confirmed the homozygous derived variant genotype fully concordant with the salmiak phenotype. We suggest the newly identified variant be designated as wsal for “w salmiak”.
Patterns of opioid use after surgical discharge: a multicentre, prospective cohort study in 25 countries
Authors:   TASMAN Collaborative
Published: May 2024

SummaryBackgroundExcessive opioid prescribing following surgery contributes to the growing opioid crisis. Prescribing practices are modifiable, yet data to guide appropriate prescription of opioids at surgical discharge remain sparse. This study aimed to evaluate factors associated with opioid consumption following discharge from surgery.MethodsAn international prospective multicentre cohort study was performed recruiting adult patients undergoing common general, orthopaedic, gynaecological and urological surgery, with follow‐up 7 days after discharge. The primary outcome measures were the quantities of prescribed and consumed opioids in oral morphine milligram equivalents. Descriptive and multivariable analyses were performed to investigate factors associated with the primary outcome measures.ResultsThis analysis included 4273 patients from 144 hospitals in 25 countries. Overall, 1311 (30.7%) patients were prescribed opioids at discharge. For those patients prescribed opioids, mean (SD) 179 (240) oral morphine milligram equivalents were prescribed, yet only 81 (145) oral morphine milligram equivalents were consumed within the first 7 days after discharge. An increased dose of opioids prescribed at discharge was associated with an increased dose of opioids consumed during the follow‐up period (β = 0.33 (95%CI 0.31–0.34), p < 0.001). The risk of prescribing more opioids than patients consumed increased as quantities of opioids prescribed at discharge exceeded 100 oral morphine milligram equivalents, independent of patient comorbidity, procedure and pain. Patients were prescribed more than twice the quantity of opioids they consumed in the first 7 days following discharge from surgery.ConclusionsOur data suggest that the current quantities of opioids provided at discharge exceed patient needs and may contribute to increasing community opioid use and circulation.
SNHG15‐mediated feedback loop interplays with HNRNPA1/SLC7A11/GPX4 pathway to promote gastric cancer progression
Authors: Duan Yantao , Yan Yonghao , Fu Hongbing , Dong Yang , Li Kun , Ye Zaisheng , Dou Yi , Huang Binhao , Kang Wei , Wei Gong‐Hong , Cai Qiliang , Xu Dazhi , Zhou Donglei 
Published: May 2024

AbstractDysregulation of long noncoding RNA (lncRNA) expression plays a pivotal role in the initiation and progression of gastric cancer (GC). However, the regulation of lncRNA SNHG15 in GC has not been well studied. Mechanisms for ferroptosis by SNHG15 have not been revealed. Here, we aimed to explore SNHG15‐mediated biological functions and underlying molecular mechanisms in GC. The novel SNHG15 was identified by analyzing RNA‐sequencing (RNA‐seq) data of GC tissues from our cohort and TCGA dataset, and further validated by qRT‐PCR in GC cells and tissues. Gain‐ and loss‐of‐function assays were performed to examine the role of SNHG15 on GC both in vitro and in vivo. SNHG15 was highly expressed in GC. The enhanced SNHG15 was positively correlated with malignant stage and poor prognosis in GC patients. Gain‐ and loss‐of‐function studies showed that SNHG15 was required to affect GC cell growth, migration and invasion both in vitro and in vivo. Mechanistically, the oncogenic transcription factors E2F1 and MYC could bind to the SNHG15 promoter and enhance its expression. Meanwhile, SNHG15 increased E2F1 and MYC mRNA expression by sponging miR‐24‐3p. Notably, SNHG15 could also enhance the stability of SLC7A11 in the cytoplasm by competitively binding HNRNPA1. In addition, SNHG15 inhibited ferroptosis through an HNRNPA1‐dependent regulation of SLC7A11/GPX4 axis. Our results support a novel model in which E2F1‐ and MYC‐activated SNHG15 regulates ferroptosis via an HNRNPA1‐dependent modulation of the SLC7A11/GPX4 axis, which serves as the critical effectors in GC progression, and provides a new therapeutic direction in the treatment of GC.
Dysfunction of CD8+ T cells around tumor cells leads to occult lymph node metastasis in NSCLC patients
Authors: Li Chaozhuo , Hu Mengyu , Cai Siqi , Yang Guanqun , Yang Liying , Jing Hongbiao , Xing Ligang , Sun Xiaorong 
Published: May 2024

AbstractOccult lymph node metastasis (OLNM) is one of the main causes of regional recurrence in inoperable N0 non‐small cell lung cancer (NSCLC) patients following stereotactic ablation body radiotherapy (SABR) treatment. The integration of immunotherapy and SABR (I‐SABR) has shown preliminary efficiency in mitigating this recurrence. Therefore, it is necessary to explore the functional dynamics of critical immune effectors, particularly CD8+ T cells in the development of OLNM. In this study, tissue microarrays (TMAs) and multiplex immunofluorescence (mIF) were used to identify CD8+ T cells and functional subsets (cytotoxic CD8+ T cells/predysfunctional CD8+ T cells (CD8+ Tpredys)/dysfunctional CD8+ T cells (CD8+ Tdys)/other CD8+ T cells) among the no lymph node metastasis, OLNM, and clinically evident lymph node metastasis (CLNM) groups. As the degree of lymph node metastasis escalated, the density of total CD8+ T cells and CD8+ Tdys cells, as well as their proximity to tumor cells, increased progressively and remarkably in the invasive margin (IM). In the tumor center (TC), both the density and proximity of CD8+ Tpredys cells to tumor cells notably decreased in the OLNM group compared with the group without metastasis. Furthermore, positive correlations were found between the dysfunction of CD8+ T cells and HIF‐1α+CD8 and cancer microvessels (CMVs). In conclusion, the deterioration in CD8+ T cell function and interactive dynamics between CD8+ T cells and tumor cells play a vital role in the development of OLNM in NSCLC. Strategies aimed at improving hypoxia or targeting CMVs could potentially enhance the efficacy of I‐SABR.
Chronic Prurigo
Authors: Thünemann Julia , Müller Svenja , Steinbrink Kerstin , Ständer Sonja , Zeidler Claudia 
Published: May 2024

SummaryChronic prurigo (CPG) is a neuroinflammatory dermatosis characterized by prolonged pruritus lasting more than 6 weeks, pruriginous skin lesions, and repeated scratching.Patients with CPG suffer significantly from psychological distress and a marked impairment in their quality of life. The most common subtype of CPG is chronic nodular prurigo (CNPG, also called prurigo nodularis).In addition to the clinical features of CPG and the burden of disease, this CME article provides an overview of the significant advances in understanding the pathophysiology, including the associated therapeutic options for CPG. Dupilumab is the first approved therapy for moderate and severe CNPG to date from the European Medicines Agency (EMA) and the US Food & Drug Administration (FDA). It also highlights other agents currently being studied in Phase II and Phase III clinical, randomized, placebo‐controlled trials. These include biologics such as nemolizumab (anti‐IL‐31‐RA‐mAb), vixarelimab/KPL‐716 (anti‐Oncostatin‐M receptor β‐mAb), and barzolvolimab/CDX‐0159 (anti‐KIT‐mAb), as well as Janus kinase inhibitors such as povorcitinib/INCB054707 and abrocitinib, and opioid modulators such as nalbuphine.
Can the galling mite Eriophyes tiliae alter the phenolic profile and antioxidant capacity of Tilia platyphyllos in a high UV radiation environment?
Authors: Guedes Lubia M. , Rodríguez‐Cerda Lorena , Gavilán Elvis , Aguilera Narciso 
Published: May 2024

AbstractLeaves of large‐leaved lime, Tilia platyphyllos Scop. (Malvaceae), harbor colonies of Eriophyes tiliae (Pagenstecher) (Acari: Eriophyidae), capable of modifying the leaf structure (inducer of nail‐galls) and physiology. The aerial organs of T. platyphyllos have traditionally been consumed for their high antioxidant capacity, related to the rich polyphenol profile. Here, we investigated the polyphenol profiles and antioxidant activity of T. platyphyllos non‐galled leaves and galls to determine the changes induced by the mite in a Chilean environment with high ultraviolet (UV) radiation. Phenolic compound identification in the methanol extracts of non‐galled leaves and galls was carried out through HPLC. Furthermore, the antioxidant activity of the extracts was quantified through spectrophotometry, and it was compared with the standards of the three major phenolic compounds of both organs. Nine phenolic compounds were detected in non‐galled leaves and galls, with no differences between the two organs, except for the absence of gallic acid in galls. The concentration of phenolic compounds did differ significantly between the two conditions. In leaf galls, epicatechin and cyanidin‐3‐glucoside concentrations and antioxidant capacity increased significantly compared to non‐galled leaves. As chlorogenic acid and cyanidin‐3‐glucoside are active phenols responding to UV radiation stress, their concentration in T. platyphyllos leaves could be a response to the high UV radiation occurring in Chile during spring and summer. The phenolic compounds detected here have been reported to be potent antioxidants, that are probably potentiated by E. tiliae for its own protection against UV‐B radiation, as the two most abundant compounds in the galls exhibited the highest capacity to reduce ABTS and DPPH radicals. However, we do not rule out the participation of phenolics in protection against natural enemies of the gall mite, as chlorogenic acid and epicatechin are powerful antifeedants.
Plant‐mediated facilitation to conspecifics and heterospecifics by two phloem‐feeding maize pests: The influence of insect identity and order of arrival
Authors: de Oliveira Marina Chaves , Morales‐Silva Tiago , Pereira Patricia , Pec Marvin , de Souza Lívia Aparecida , Peñaflor Maria Fernanda G. V. 
Published: May 2024

Abstract
Herbivory by insects leads to changes in both primary and secondary plant metabolism, ultimately impacting subsequent interactions. Some phloem‐feeding insects have developed mechanisms to suppress herbivore‐induced plant defences and manipulate the quality of host phloem sap, thereby facilitating their establishment on the host plant. Based on evidence that the specialized phloem‐feeding insects, Dalbulus maidis and Rhopalosiphum maidis, can suppress herbivore‐induced defences in maize, we hypothesized that infestation by either of these pests in maize would facilitate the colonization and establishment of subsequent conspecific and heterospecific individuals. We conducted a series of behavioural assays with these insects in both laboratory and greenhouse settings.
The corn leafhopper D. maidis preferred previously infested plants as hosts, but it did not discriminate between conspecific‐ or heterospecific‐infested plants for colonization. However, when selecting a host for oviposition, it preferred depositing eggs on conspecific‐infested plants, which were the hosts that the offspring performed better compared to both non‐infested and heterospecific‐infested plants.
The corn aphid R. maidis also preferred previously infested plants over non‐infested plants as hosts. However, the aphid preferred heterospecific‐infested over conspecific‐infested plants, even though heterospecific‐infested plants were hosts of lower quality to aphids. Aphids performed better on conspecific‐infested plants, followed by heterospecific‐infested plants, when compared to non‐infested plants.
Our results confirm that previous colonization by the specialized phloem‐feeding insects facilitates subsequent infestations by conspecifics and heterospecifics. However, the facilitation for heterospecifics was asymmetric, as colonization by D. maidis facilitated infestations by R. maidis more than the reverse sequence of infestation.