bluuebunny/crossref_metadata_2025_split huggingface dataset:
Examining Patient Risk Factors, Comorbid Conditions, Participation, and Physician Referrals to a Rural Diabetes Self-management Education Program
Authors: Ruppert Kristine , Uhler Amy , Siminerio Linda 
Published: May 2010

Purpose The purpose of this study was to examine patients’ diabetes risk factors, comorbid conditions, and patient participation in and primary care practitioner (PCP) referrals to a rural diabetes self-management education (DSME) program. Methods A total of 295 patients in a rural community were identified by their PCP as having type 2 diabetes (T2D). Using patient information that was collected and entered into a diabetes data management system, patients’ risk factors, comorbid conditions, and patient participation in and PCP referral patterns to a DSME program were examined. Results Of the 295 patients with T2D, 162 (65%) reported that they had never received any DSME services. Despite educator efforts to improve patient participation and PCP awareness of local DSME services, 123 (76%) of the 162 patients never received a subsequent referral for DSME. Those patients who did receive a referral had a higher number of risk factors and comorbid conditions than those who did not receive a referral. Eighty-three percent of the patients who received a PCP referral attended the DSME program. Conclusions The findings reaffirm concerns that DSME patient participation and PCP referral practices are poor. Advocacy efforts should force policies and procedures that will make DSME a mandatory service and universally accessible. Unless referral practices are attended to, it is doubtful that the United States will reach the Healthy People 2010 objective for diabetes education.
Psychosocial Resources and Barriers to Self-management in African American Adolescents With Type 2 Diabetes
Authors: Auslander Wendy F. , Sterzing Paul R. , Zayas Luis E. , White Neil H. 
Published: May 2010

Purpose The purpose of this qualitative study was to identify psychosocial resources and barriers to self-management among African American adolescents with type 2 diabetes and their mothers. Methods African American adolescents (n = 10) aged 14 to 19 years old with type 2 diabetes for >1 year and their mothers (n = 10) were recruited from the pediatric diabetes clinic of a large medical center practice. Participants were independently interviewed and responded to a series of open-ended questions concerning illness experiences and resources and barriers to diabetes self-management. This study used a modified grounded theory approach to data coding and analysis. Results Adolescents and mothers shared similar perceptions of resources and barriers to self-management. Resources included mother’s role as the primary support person, emergence of greater self-efficacy and coping over time, family recognition of the seriousness of diabetes, and the presence of supportive peers. Barriers included comorbidity, dietary and other regimen challenges, negative peer influences, and financial problems. Conclusions Resources and barriers identified in this study represent the multiple contexts that influence type 2 diabetes (eg, individual, family, peer, economic, and cultural).
Topiramate intervention to prevent transformation of episodic migraine: The topiramate INTREPID study
Authors: Lipton Richard B , Silberstein Stephen , Dodick David , Cady Roger , Freitag Fred , Mathew Ninan , Biondi David M , Ascher Steven , Olson William H , Hulihan Joseph 
Published: May 2010

Objective: The study sought to evaluate whether topiramate prevents development of chronic daily headache (CDH, ≥15 headache days per month) in adult subjects with high-frequency episodic migraine (HFEM, 9–14 migraine headache days/month). A secondary objective was to assess the efficacy of topiramate as preventive migraine treatment in this population. Methods: This was a multicenter, randomized, double-blind, placebo-controlled study comparing topiramate 100 mg/day and placebo for 26 weeks. The primary efficacy variable was new-onset CDH at month 6. Secondary efficacy measures included migraine and headache days. Adverse events (AEs) were evaluated. Results: A total of 159 topiramate subjects and 171 placebo subjects were efficacy-evaluable. At month 6, 1.4% of topiramate subjects versus 2.3% of placebo subjects had CDH ( p = .589). Compared with placebo, topiramate treatment was associated with statistically significant reductions in mean number of migraine days (6.6 vs. 5.3/28 days; p = .001) and headache days (6.6 vs 5.3/28 days; p = .001). Most commonly reported AEs in the topiramate versus placebo group included paresthesia (32.4% vs. 7.0%), fatigue (14.8% vs. 8.6%), dizziness (11.4% vs. 7.6%) and nausea (10.8% vs. 9.2%). Conclusion: Topiramate 100 mg/day did not prevent the development of CDH at six months in subjects with HFEM. Topiramate was effective in reducing headache days and migraine headache days and generally well tolerated.
Anteromedial Impingement in the Ankle Joint
Authors: Murawski Christopher D. , Kennedy John G. 
Published: May 2010

Background Arthroscopic debridement is a well-accepted method of removing osseous and/or soft tissue impingement from the ankle joint. To the best of the authors’ knowledge, this is the first case series reporting the outcomes following arthroscopic resection of anteromedial impingement. Purpose The authors report the results at a minimum 2-year follow-up of 41 patients who underwent arthroscopic resection for anteromedial impingement. Study Design Case series; Level of evidence, 4. Methods Arthroscopic surgery for anteromedial impingement was performed on 43 patients under the care of the senior surgeon between January 2005 and July 2007; 41 patients were included in the present study. All patients were assessed preoperatively and postoperatively using the American Orthopaedic Foot and Ankle Society (AOFAS) hindfoot and Short Form 36 (SF-36v2) outcome scores. The mean patient age at the time of surgery was 31.12 years (range, 13-57 years). The mean follow-up time was 34.41 months (range, 24-52 months). Thirty-four patients (83%) were competing at some level of athletic sport. Results Thirty-eight patients (93%) were satisfied with the procedure. The AOFAS scores improved from 62.83 points preoperatively to 91.17 points postoperatively (range, 61-100 points) (P < .001). The SF-36v2 scores improved from 61.54 points preoperatively to 92.21 points postoperatively (range, 58-100 points) (P = .002). All but 1 patient returned to their prior level of sporting activity. Three patients (7%) reported a complication. Conclusion Arthroscopic resection for anteromedial impingement provides excellent functional outcomes, thereby allowing the athlete an expedited return to sport at previous levels of competition.
On the values guiding the French practice of journalism: Interviews with thirteen war correspondents
Authors: Boudana Sandrine 
Published: May 2010

What are the values guiding the French practice of journalism? What is the place of objectivity among these values? These questions were asked of 13 war correspondents working for eight of the leading French newspapers. While objectivity is rejected as either an unattainable standard or an undesirable norm, it appears that a definition of objectivity is lacking and that this notion is often mistaken for neutrality. Three different conceptions of objectivity-as-neutrality emerge from the interviewees’ discourses: as a separation between facts and commentaries, as cautiousness in labeling and as a balance between the parties. Can or should one of these conceptions serve as a guideline in the daily practice of journalism? The interviewees seem to be divided on this question and several propose moral values such as honesty or modesty as alternatives. The reference to moral values in turn proves problematic insofar as the focus on the journalists’ attitudes or intentions fails to address responsibility for highly consequential actions. In the end, when discussing specific dilemmas that they themselves have faced, most of the journalists quote accuracy and fairness as criteria for the evaluation of journalistic performance. ‘ Etre juste’ — meaning both to be accurate ( justesse) and to be fair ( justice) — is what is expected of a professional journalist.
The relationship between angiotensin converting enzyme gene I/D polymorphism and QT dispersion in patients with hypertrophic cardiomyopathy
Authors: Kaya Cansin Tulunay , Gurlek Adalet , Altin Timucin , Kilickap Mustafa , Karabulut Halil Gurhan , Turhan Sibel , Ozcan Ozgur , Bokesoy Isik , Oral Dervis , Erol Cetin 
Published: May 2010

Introduction: Hypertrophic cardiomyopathy (HCM) is characterized by disorganized myocardial architecture, and may cause ventricular arrhythmias and sudden death. The angiotensin-converting enzyme (ACE) with two deletion alleles (DD genotype) has been proposed to be associated with increased myocardial collagen content. We evaluated QT dispersion (QTd), which reflects regional differences in ventricular repolarization, in HCM patient and controls among the three different ACE genotypes. Materials and methods: Sixty-three patients with HCM and 20 healthy subjects were included in the study. QT parameters were measured from 12 lead electrocardiograms. ACE genotypes were determined from the DNA extracted from peripheral blood by a polymerase chain reaction (PCR) method. QT parameters were compared among the three ACE genotypes both in HCM patients and controls. Results: Median ages were similar in HCM and control groups. QTd and corrected QTd (QTcd) were significantly greater in the HCM group compared with the controls. The frequencies of each genotype were similar in both groups. Although QTd and QTcd did not differ among the three genotypes in the control subjects, they were significantly greater in patients with DD genotype compared with other genotypes in the HCM group. Conclusion: QTd and QTcd are increased in patients with HCM, especially in those with the DD genotype.
Minimizing Injuries and Enhancing Performance in Golf Through Training Programs
Authors: Meira Erik P. , Brumitt Jason 
Published: May 2010

Context: Golf is a popular sport, particularly in older populations. Regardless of age and skill level, golfers risk injury to the back, shoulder, wrist and hand, elbow, and knee. Because of the unique compressive, shear, rotational, and lateral bending forces created in the lumbar region during the golf swing, the primary sport-related malady experienced by amateurs and professionals is low back pain. Extrinsic and intrinsic injury risk factors have been reported in the literature. A growing body of evidence supports the prescription of strength training routines to enhance performance and reduce the risk of injury. Evidence Acquisition: Relevant studies were reviewed on golf injuries, swing mechanics, training routines, and general training program design. The following electronic databases were used to identify research relevant to this report: MEDLINE (from 1950–November 2009), CINAHL (1982–November 2009), and SPORTDiscus (1830–November 2009). Results: Injuries may be associated with lack of warm-up, poor trunk flexibility and strength, faulty swing technique, and overuse. Conclusions: Implementing a training program that includes flexibility, strength, and power training with correction of faulty swing mechanics will help the golfer reduce the likelihood of injury and improve overall performance.
Homologous recombination as a resistance mechanism to replication-induced double-strand breaks caused by the antileukemia agent CNDAC
Authors: Liu Xiaojun , Wang Yaqing , Benaissa Sherri , Matsuda Akira , Kantarjian Hagop , Estrov Zeev , Plunkett William 
Published: May 2010

AbstractThe nucleoside analog 2′-C-cyano-2′-deoxy-1-β-D-arabino-pentofuranosyl-cytosine (CNDAC), currently in clinical trials for hematologic malignancies, has a novel action mechanism of causing a single-strand break after its incorporation into DNA. Double-strand breaks (DSBs) are generated thereafter in vivo and, if not repaired, pose lethal impact on cell survival. This study sought to define the mechanisms by which CNDAC-induced DSBs are formed and repaired. We demonstrated that single-strand breaks induced by CNDAC incorporation into DNA were converted to DSBs when cells progressed into the subsequent S-phase. CNDAC-induced DSBs were products of replication, rather than a consequence of apoptosis. ATM, the activator of homologous recombination (HR), was essential for cell survival after CNDAC treatment in cell lines and in primary acute myeloid leukemia samples, as were the HR components, Rad51, Xrcc3, and Brca2. Furthermore, formation of sister chromatid exchanges, a hallmark of HR, increased significantly after CNDAC-treated cells had progressed into a second replication cycle. In contrast, neither the replication stress sensor ATR nor DNA-PK, the initiator of nonhomologous end-joining of DSB, was involved in repair of CNDAC-induced damage. Together, these results indicate that HR, but not nonhomologous end-joining, is the major repair or survival mechanism for DNA damage caused by CNDAC.
The Kunitz-3 domain of TFPI-α is required for protein S–dependent enhancement of factor Xa inhibition
Authors: Ndonwi Matthew , Tuley Elodee A. , Broze George J. 
Published: May 2010

AbstractProtein S (PS) enhances the inhibition of factor Xa (FXa) by tissue factor pathway inhibitor-α (TFPI-α) in the presence of Ca2+ and phospholipids. Altered forms of recombinant TFPI-α were used to determine the structures within TFPI-α that may be involved in this PS-dependent effect. Wild-type TFPI-α (TFPIWT), TFPI-α lacking the K3 domain (TFPI-ΔK3), and TFPI-α containing a single amino acid change at the putative P1 residue of K3 (R199L, TFPIK3P1) produced equivalent FXa inhibition in the absence of PS, whereas the response in FXa inhibition produced by PS was reduced with TFPIK3P1 (EC50 61.8 ± 13.4nM vs 8.0 ± 0.4nM for TFPIWT) and not detectable with TFPI-ΔK3. Ligand blotting and surface plasmon resonance experiments demonstrated that FXa bound TFPIWT and TFPI-ΔK3 but not the isolated K3 domain, whereas PS bound TFPIWT and the K3 domain but not TFPI-ΔK3. Addition of TFPIWT, TFPIK3P1, or TFPI-ΔK3 produced comparable prolongation of FXa-induced coagulation in PS-deficient plasma, but the anticoagulant effect of TFPIWT was substantially greater than that of TFPIK3P1 > TFPI-ΔK3 in normal plasma and PS-deficient plasma reconstituted with PS. We conclude that the PS-mediated enhancement of FXa inhibition by TFPI-α involves an interaction between PS and TFPI-α, which requires the K3 domain of TFPI-α.
CD99 and CD99L2 act at the same site as, but independently of, PECAM-1 during leukocyte diapedesis
Authors: Bixel M. Gabriele , Li Hang , Petri Bjoern , Khandoga Alexander G. , Khandoga Andrej , Zarbock Alexander , Wolburg-Buchholz Karen , Wolburg Hartwig , Sorokin Lydia , Zeuschner Dagmar , Maerz Sigrid , Butz Stefan , Krombach Fritz , Vestweber Dietmar 
Published: May 2010

AbstractLeukocyte extravasation depends on various adhesion receptors at endothelial cell contacts. Here we have analyzed how mouse CD99 and CD99L2 cooperate with PECAM-1. We found that antibodies against mouse CD99 and PECAM-1 trap neutrophils between endothelial cells in in vitro transmigration assays. A sequential function, as has been suggested for human PECAM-1 and CD99, could not be demonstrated. In contrast to these in vitro results, blocking CD99 or CD99L2 or gene disruption of PECAM-1 trapped neutrophils in vivo between endothelial cells and the underlying basement membrane as revealed by electron microscopy and by 3-dimensional confocal fluorescence microscopy in the inflamed cremaster tissue. Leukocyte extravasation was inhibited in interleukin-1β-inflamed peritoneum and in the cremaster by PECAM-1 gene disruption and was further attenuated by blocking antibodies against CD99 and CD99L2. In addition, CD99 and CD99L2 were required for leukocyte extravasation in the cremaster after stimulation with tumor necrosis factor-α, where the need for PECAM-1 is known to be bypassed. We conclude that CD99 and CD99L2 act independently of PECAM-1 in leukocyte extravasation and cooperate in an independent way to help neutrophils overcome the endothelial basement membrane.
Hydroxyurea in essential thrombocythemia: rate and clinical relevance of responses by European LeukemiaNet criteria
Authors: Carobbio Alessandra , Finazzi Guido , Antonioli Elisabetta , Vannucchi Alessandro M. , Barosi Giovanni , Ruggeri Marco , Rodeghiero Francesco , Delaini Federica , Rambaldi Alessandro , Barbui Tiziano 
Published: May 2010

Abstract
A definition of response by cytoreductive therapy in essential thrombocythemia was recently provided by the European LeukemiaNet (ELN). Complete, partial, or no clinicohematologic responses were defined on the bases of platelet count, disease-related symptoms, spleen size, and white blood cell count. To provide estimates and clinical correlation of responses according to these criteria, we retrospectively examined 416 essential thrombocythemia patients treated with hydroxyurea for at least 12 months. Complete response, partial response, and no response were 25%, 58%, and 17%, respectively. Age more than 60 years and JAK2V617F mutation were significant predictors of response. After a median follow-up of 3.9 years, we registered 23 deaths, 16 hematologic transformations, and 27 thrombotic events (rate, 1.66% patients/year). Age, previous thrombosis, leukocytosis (white blood cell count > 10 × 109/L), but not ELN responses, were independently associated with higher risk of thrombosis. The actuarial probability of thrombosis was significantly influenced by leukocytosis (P = .017) and not by platelet count, indicating that platelet number does not seem of prime relevance in the definition of ELN response.
Detecting microRNA activity from gene expression data
Authors: Madden Stephen F , Carpenter Susan B , Jeffery Ian B , Björkbacka Harry , Fitzgerald Katherine A , O'Neill Luke A , Higgins Desmond G 
Published: May 2010

Abstract

Background
MicroRNAs (miRNAs) are non-coding RNAs that regulate gene expression by binding to the messenger RNA (mRNA) of protein coding genes. They control gene expression by either inhibiting translation or inducing mRNA degradation. A number of computational techniques have been developed to identify the targets of miRNAs. In this study we used predicted miRNA-gene interactions to analyse mRNA gene expression microarray data to predict miRNAs associated with particular diseases or conditions.

Results
Here we combine correspondence analysis, between group analysis and co-inertia analysis (CIA) to determine which miRNAs are associated with differences in gene expression levels in microarray data sets. Using a database of miRNA target predictions from TargetScan, TargetScanS, PicTar4way PicTar5way, and miRanda and combining these data with gene expression levels from sets of microarrays, this method produces a ranked list of miRNAs associated with a specified split in samples. We applied this to three different microarray datasets, a papillary thyroid carcinoma dataset, an in-house dataset of lipopolysaccharide treated mouse macrophages, and a multi-tissue dataset. In each case we were able to identified miRNAs of biological importance.

Conclusions
We describe a technique to integrate gene expression data and miRNA target predictions from multiple sources.
Conservation of MAP kinase activity and MSP genes in parthenogenetic nematodes
Authors: Heger Peter , Kroiher Michael , Ndifon Nsah , Schierenberg Einhard 
Published: May 2010

Abstract
Background
MAP (mitogen-activated protein) kinase activation is a prerequisite for oocyte maturation, ovulation and fertilisation in many animals. In the hermaphroditic nematode Caenorhabditis elegans, an MSP (major sperm protein) dependent pathway is utilised for MAP kinase activation and successive oocyte maturation with extracellular MSP released from sperm acting as activator. How oocyte-to-embryo transition is triggered in parthenogenetic nematode species that lack sperm, is not known.

Results
We investigated two key elements of oocyte-to-embryo transition, MSP expression and MAP kinase signaling, in two parthenogenetic nematodes and their close hermaphroditic relatives. While activated MAP kinase is present in all analysed nematodes irrespective of the reproductive mode, MSP expression differs. In contrast to hermaphroditic or bisexual species, we do not find MSP expression at the protein level in parthenogenetic nematodes. However, genomic sequence analysis indicates that functional MSP genes are present in several parthenogenetic species.

Conclusions
We present three alternative interpretations to explain our findings. (1) MSP has lost its function as a trigger of MAP kinase activation and is not expressed in parthenogenetic nematodes. Activation of the MAP kinase pathway is achieved by another, unknown mechanism. Functional MSP genes are required for occasionally emerging males found in some parthenogenetic species. (2) Because of long-term disadvantages, parthenogenesis is of recent origin. MSP genes remained intact during this short intervall although they are useless. As in the first scenario, an unknown mechanism is responsible for MAP kinase activation. (3) The molecular machinery regulating oocyte-to-embryo transition in parthenogenetic nematodes is conserved with respect to C. elegans, thus requiring intact MSP genes. However, MSP expression has been shifted to non-sperm cells and is reduced below the detection limits, but is still sufficient to trigger MAP kinase activation and embryogenesis.
Generation of microsatellite repeat families by RTE retrotransposons in lepidopteran genomes
Authors: Tay Wee Tek , Behere Gajanan T , Batterham Philip , Heckel David G 
Published: May 2010

Abstract

Background
Developing lepidopteran microsatellite DNA markers can be problematical, as markers often exhibit multiple banding patterns and high frequencies of non-amplifying "null" alleles. Previous studies identified sequences flanking simple sequence repeat (SSR) units that are shared among many lepidopteran species and can be grouped into microsatellite-associated DNA families. These families are thought to be associated with unequal crossing-over during DNA recombination or with transposable elements (TEs).

Results
We identified full-length lepidopteran non-LTR retrotransposable elements of the RTE clade in Heliconius melpomene and Bombyx mori. These retroelements possess a single open reading frame encoding the Exonuclease/Endonuclease/Phosphatase and the Reverse Transcriptase/nLTR domains, a 5' UTR (untranslated region), and an extremely short 3' UTR that regularly consists of SSR units. Phylogenetic analysis supported previous suggestions of horizontal transfer among unrelated groups of organisms, but the diversity of lepidopteran RTE elements appears due to ancient divergence of ancestral elements rather than introgression by horizontal transfer. Similarity searches of lepidopteran genomic sequences in GenBank identified partial RTE elements, usually consisting of the 3' terminal region, in 29 species. Furthermore, we identified the C-terminal end of the Reverse Transcriptase/nLTR domain and the associated 3' UTR in over 190 microsatellite markers from 22 lepidopteran species, accounting for 10% of the lepidopteran microsatellites in GenBank. Occasional retrotransposition of autonomous elements, frequent retrotransposition of 3' partial elements, and DNA replication slippage during retrotransposition offers a mechanistic explanation for the association of SSRs with RTE elements in lepidopteran genomes.

Conclusions
Non-LTR retrotransposable elements of the RTE clade therefore join a diverse group of TEs as progenitors of SSR units in various organisms. When microsatellites are isolated using standard SSR enrichment protocols and primers designed at complementary repeated regions, amplification from multiple genomic sites can cause scoring difficulties that compromise their utility as markers. Screening against RTE elements in the isolation procedure provides one strategy for minimizing this problem.