bluuebunny/crossref_metadata_2025_split huggingface dataset:
Responsiveness of the major birch allergen Bet v 1 scaffold to the gastric environment: Impact on structure and allergenic activity
Authors: Sancho Ana I. , Wangorsch Andrea , Jensen Bettina M. , Watson Andrew , Alexeev Yuri , Johnson Phil E. , Mackie Alan R. , Neubauer Angela , Reese Gerald , Ballmer‐Weber Barbara , Hoffmann‐Sommergruber Karin , Skov Per S. , Vieths Stefan , Mills Elisabeth N. Clare 
Published: July 2011

AbstractScope: Four Bet v 1 homologous food allergens from celeriac (rApi g 1), apple (rMal d 1), peach (rPru p 1) and hazelnut (rCor a 1), were used to probe the structural responsiveness of the Bet v 1 scaffold to gastric digestion conditions and its impact on allergenicity.Methods and results: Low pH induced conformational changes of all homologues, which was reduced at physiological ionic strength for all except rPru p 1 as observed by circular dichroism (CD)‐spectroscopy. The homologues were rapidly digested by pepsin, losing their IgE binding activity, although the kinetics and patterns of digestion varied subtly between homologues, rApi g 1 being the most stable. We have demonstrated for the first time that gastric phosphatidyl‐choline (PC) induced conformational changes in all homologues but only rMal d 1 penetrated the PC vesicles as detected by fluorescence polarization, slowing its digestion and retaining more of its allergenic activity. PC enhanced basophil activation of all digested allergens except rApi g 1.Conclusion: The Bet v 1 scaffold is generally susceptible to low pH and pepsinolysis and interacts with PC vesicles, properties which can explain effects of the gastric environment on their allergenicity. These data show the importance of including surfactants in model digestion systems.
Regular endurance exercise improves the diminished hepatic carnitine status in mice fed a high‐fat diet
Authors: Ringseis Robert , Mooren Frank‐Christoph , Keller Janine , Couturier Aline , Wen Gaiping , Hirche Frank , Stangl Gabriele I. , Eder Klaus , Krüger Karsten 
Published: July 2011

AbstractScope: Metabolic stress induced by chronic high‐fat (HF) diet feeding or genetically induced diabetes impairs carnitine status. Herein, we tested the hypothesis that regular endurance exercise (EE) improves the HF diet‐induced impairment of carnitine status through stimulating the expression of hepatic genes involved in carnitine synthesis and uptake.Methods and results: Eighteen male C57BL/6 mice were assigned to three groups: group S received a standard diet, group HF received a HF diet, and group HF+EE received an HF diet and was regularly exercised on a treadmill. After 10 wk, mice of the HF and the HF+EE groups were highly obese and insulin resistant compared with mice of the S group (p<0.05), but mice of the HF+EE group were less insulin resistant than those of the HF group (p<0.05). The HF group had lower carnitine concentrations and mRNA and protein levels of genes involved in carnitine synthesis and uptake in the liver than the S group (p<0.05), whereas these parameters did not differ between the S group and the HF+EE group.Conclusion: These findings indicate that regular EE reverses an HF diet‐induced impairment of hepatic carnitine content by stimulating hepatic carnitine synthesis and uptake.
The multiple hats of Vasa: Its functions in the germline and in cell cycle progression
Authors: Yajima Mamiko , Wessel Gary M. 
Published: July 2011

AbstractVasa, an ATP‐dependent RNA helicase, is broadly conserved among various organisms from cnidarians to mammals. It has a rich history of utility as a germline marker, and is believed to function as a positive translational regulator in the determination and maintenance of germline cells. Studies in non‐model organisms, however, revealed that Vasa is also present in somatic cells of many tissues. In many cases these cells are multipotent, are non‐germline associated, and give rise to a variety of different tissue types. Recent work now also demonstrates that Vasa functions in the regulation of the cell cycle. Here, we discuss this newly described function of Vasa in mitotic and meiotic cell cycles, and we address the conundrum created within these observations, that is, that most cells are mitotically independent of Vasa, yet when Vasa is present in a cell, it appears to be essential for cell cycle progression. Mol. Reprod. Dev. 78:861–867, 2011. © 2011 Wiley Periodicals, Inc.
Combining resources to obtain a comprehensive survey of the bovine embryo transcriptome through deep sequencing and microarrays
Authors: Robert Claude , Nieminen Julie , Dufort Isabelle , Gagné Dominic , Grant Jason R. , Cagnone Gaël , Plourde Dany , Nivet Anne‐Laure , Fournier Éric , Paquet Éric , Blazejczyk Michal , Rigault Philippe , Juge Nicolas , Sirard Marc‐André 
Published: July 2011

AbstractWhile most assisted reproductive technologies (ART) are considered routine for the reproduction of species of economical importance, such as the bovine, the impact of these manipulations on the developing embryo remains largely unknown. In an effort to obtain a comprehensive survey of the bovine embryo transcriptome and how it is modified by ART, resources were combined to design an embryo‐specific microarray. Close to one million high‐quality reads were produced from subtracted bovine embryo libraries using Roche 454 Titanium deep sequencing technology, which enabled the creation of an augmented bovine genome catalog. This catalog was enriched with bovine embryo transcripts, and included newly discovered indel type and 3′UTR variants. Using this augmented bovine genome catalog, the EmbryoGENE Bovine Microarray was designed and is composed of a total of 42,242 probes, including 21,139 known reference genes; 9,322 probes for novel transcribed regions (NTRs); 3,677 alternatively spliced exons; 3,353 3′‐tiling probes; and 3,723 controls. A suite of bioinformatics tools was also developed to facilitate microrarray data analysis and database creation; it includes a quality control module, a Laboratory Information Management System (LIMS) and microarray analysis software. Results obtained during this study have already led to the identification of differentially expressed blastocyst targets, NTRs, splice variants of the indel type, and 3′UTR variants. We were able to confirm microarray results by real‐time PCR, indicating that the EmbryoGENE bovine microarray has the power to detect physiologically relevant changes in gene expression. Mol. Reprod. Dev. 78:651–664, 2011. © 2011 Wiley‐Liss, Inc.
Caffeine alleviates the deterioration of Ca2+ release mechanisms and fragmentation of in vitro‐aged mouse eggs
Authors: Zhang Nan , Wakai Takuya , Fissore Rafael. A. 
Published: July 2011

AbstractThe developmental competence of mammalian eggs is compromised by postovulatory aging. We and others have found that in these eggs, the intracellular calcium ([Ca2+]i) responses required for egg activation and initiation of development are altered. Nevertheless, the mechanism(s) underlying this defective Ca2+ release is not well known. Here, we investigated if the function of IP3R1, the major Ca2+ release channel at fertilization, was undermined in in vitro‐aged mouse eggs. We found that in aged eggs, IP3R1 displayed reduced function as many of the changes acquired during maturation that enhance IP3R1 Ca2+ conductivity, such as phosphorylation, receptor reorganization and increased Ca2+ store content ([Ca2+]ER), were lost with increasing postovulatory time. IP3R1 fragmentation, possibly associated with the activation of caspase‐3, was also observed in these eggs. Many of these changes were prevented when the postovulatory aging of eggs was carried out in the presence of caffeine, which minimized the decline in IP3R1 function and maintained [Ca2+]ER content. Caffeine also maintained mitochondrial membrane potential, as measured by JC‐1 fluorescence. We therefore conclude that [Ca2+]i responses in aged eggs are undermined by reduced IP3R1 sensitivity, decreased [Ca2+]ER, and compromised mitochondrial function, and that addition of caffeine ameliorates most of these aging‐associated changes. Understanding the molecular basis of the protective effects of caffeine will be useful in elucidating, and possibly reversing, the signaling pathway(s) compromised by in vitro culture of eggs. Mol. Reprod. Dev. 78:684–701, 2011. © 2011 Wiley‐Liss, Inc.
QoS‐aware and compromise‐resilient key management scheme for heterogeneous wireless Internet of Things
Authors: Ren Wei 
Published: July 2011

AbstractThe Internet of Things (IOT) is envisioned as a natural evolution of the Internet, promising to enable ubiquitous connections for pervasive objects. The evolutionary merging of heterogeneous wireless networks is inevitable for smooth migration to IOT; for example, in a typical application of IOT—smart homes—there exist sensor–radio frequency identification (RFID) hybrid networks. The communication security between sensor (or RFID tags) and home control center is critical, whereas an appropriate key management scheme is a prerequisite for communication security. In this paper, we propose a compromise resilient key management scheme including key agreement schemes and key evolution policies to tackle existing remarkable asymmetry with respect to computation resources of hybrid networks. In particular, a forward and backward secure key evolution policy with formal proof is proposed. We also propose a quality of service (QoS)‐aware enhancement method by measuring several metrics such as data assurance priority, attacking risk, and remaining power percentage. Security parameter negotiation and a tuning method are proposed, based on reactive measurement in real time. Our proposed scheme is built on abstract cryptographic primitives such as trapdoor permutation, pseudorandom function, pseudorandom number generator, one‐way function and hash function so as to retain flexibility for concrete options. Security and performance for proposed key agreement schemes and key evolution policies are compared in detail. Three QoS‐aware security strategies are proposed: performance first, security second (PFSS); security first, performance second (SFPS); and performance security made balanced (PSMB). Measurement‐based negotiation of security parameters is also proposed in terms of algorithm prototype. Copyright © 2011 John Wiley & Sons, Ltd.
New methods for the estimation of Takagi–Sugeno model based extended Kalman filter and its applications to optimal control for nonlinear systems
Authors: Al‐Hadithi Basil M. , Jiménez Agustín , Matía Fernando 
Published: July 2011

SUMMARYThis paper describes new approaches to improve the local and global approximation (matching) and modeling capability of Takagi–Sugeno (T‐S) fuzzy model. The main aim is obtaining high function approximation accuracy and fast convergence. The main problem encountered is that T‐S identification method cannot be applied when the membership functions are overlapped by pairs. This restricts the application of the T‐S method because this type of membership function has been widely used during the last 2 decades in the stability, controller design of fuzzy systems and is popular in industrial control applications. The approach developed here can be considered as a generalized version of T‐S identification method with optimized performance in approximating nonlinear functions. We propose a noniterative method through weighting of parameters approach and an iterative algorithm by applying the extended Kalman filter, based on the same idea of parameters’ weighting. We show that the Kalman filter is an effective tool in the identification of T‐S fuzzy model. A fuzzy controller based linear quadratic regulator is proposed in order to show the effectiveness of the estimation method developed here in control applications. An illustrative example of an inverted pendulum is chosen to evaluate the robustness and remarkable performance of the proposed method locally and globally in comparison with the original T‐S model. Simulation results indicate the potential, simplicity, and generality of the algorithm. An illustrative example is chosen to evaluate the robustness. In this paper, we prove that these algorithms converge very fast, thereby making them very practical to use. Copyright © 2011 John Wiley & Sons, Ltd.
Ovarian clear cell carcinoma—bad endometriosis or bad endometrium?
Authors: Gounaris Ioannis , Charnock‐Jones D Stephen , Brenton James D 
Published: July 2011

AbstractIt has become increasingly clear that the four main histological subtypes of epithelial ovarian cancer (EOC), high‐grade serous, endometrioid, clear cell and mucinous, are entities with different epidemiologies, clinical presentations, responses to treatment, and ultimate outcomes. In fact, for all intents and purposes, they can be considered different diseases, their only common denominator being that they frequently involve the ovary and pelvic organs. However, clinical practice has not caught up with these insights and the treatment of EOC is that of a single disease entity. In part, this is because we lack detailed knowledge of the molecular mechanisms driving the pathogenesis of each disease, which is vital in order to develop therapeutic approaches against common driver events. In the last few years, mutations in ARID1A and PIK3CA have been described in a substantial fraction of cases of ovarian clear cell carcinoma, yet the paper by Yamamoto et al in this issue of The Journal of Pathology reveals that PIK3CA mutations can be detected in precursor endometriosis tissues. These and other recent observations underscore the importance of investigating whether mutations in the eutopic endometrium actually predispose to endometriosis and eventually to malignancy. Copyright © 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Hypertriglyceridaemia in statin‐treated type 2 diabetic patients
Authors: Hamilton SJ , Chew GT , Davis TME , Stuckey BGA , Watts GF 
Published: July 2011

AbstractIn this cross‐sectional study, we investigated the prevalence of hypertriglyceridaemia (hyperTG) in 182 statin‐treated type 2 diabetic (T2DM) patients.Predictors of hyperTG (≥2.3mmol/L) were investigated using logistic regression. The prevalence of hyperTG was 20.9%, with lower prevalence in patients with low‐density lipoprotein (LDL)‐cholesterol <2.5mmol/L (13.7%), and LDL‐cholesterol <2.0mmol/L (8.8%). The prevalence of hyperTG plus low high‐density lipoprotein (HDL)‐cholesterol (≤0.9mmol/L) was lower at 6.0%. The independent predictors of hyperTG were waist circumference (odds ratio [OR] 1.033 [95% confidence interval 1.004–1.063], p=0.027) and glucose (OR 1.30 [1.05–1.61], p=0.01), with glucose being the sole predictor in patients with LDL‐cholesterol <2.5mmol/L (OR 1.45 [1.11–1.89], p=0.01) and LDL‐cholesterol <2.0mmol/L (OR 1.59 [1.12–2.26], p=0.01).In this group of statin‐treated T2DM patients, the prevalence of hyperTG was relatively high, but lower in patients with lower LDL‐cholesterol levels. Residual hyperTG in statin‐treated patients could be addressed by therapeutic lifestyle interventions aimed at weight loss and improved glycaemic control and by further lowering of LDL‐cholesterol. Copyright © 2011 John Wiley & Sons.
Consumers find food labels confusing and too small to read
Authors: Deakin TA 
Published: July 2011

AbstractThe aim of this research was to determine whether consumers are able to read and understand food labels.A structured interview was conducted during September 2009 with 176 consumers from a cross section of the population. Consumers, from teenagers to pensioners, were interviewed in a variety of locations including a town centre, a cafe, a supermarket, a commercial workplace, a leisure centre and a fast food restaurant.The majority of respondents (n=155, 88%) try to lead a healthy lifestyle with 149 (85%) reporting that eating healthily is important to them. Over half of respondents (n=102, 58%) read food labels when purchasing food and drink. When presented with a sample of food labels, more than half of consumers (n=96, 55%) interviewed stated that they did not understand the nutritional information and 108 (61%) reported that the labelling information is too small to read. Three out of four consumers (n=134, 76%) announced that they would value educational material with an integral magnifying glass to help them read and understand food labels. There were no significant differences in the findings attributable to the location of interview.It was concluded that the majority of consumers try to lead a healthy lifestyle and eat a healthy diet but find food labels confusing and too small to read. Educational material with an integral magnifying glass may assist consumers in making healthier food choices. Copyright © 2011 John Wiley & Sons.
Triple‐B (basal‐bolus‐booster) subcutaneous insulin regimen: a pragmatic approach to managing hospital inpatient hyperglycaemia
Authors: Perera NJ , Harding AJ , Constantino MI , Molyneaux L , McGill M , Chua EL , Twigg SM , Ross GP , Yue DK 
Published: July 2011

AbstractThe objective of this study was to introduce a practical insulin protocol for hospital inpatients with hyperglycaemia.The acronym BBB emphasised the insulin supply in three components, basal, bolus (nutrition correction) and booster (blood glucose level [BGL] correction). The insulin dosage was based on patient weight and adjusted to BGL at pre‐specified times. Compliance of BGL measurements and insulin injections, and efficacy were evaluated prospectively. Fifty‐seven hospital inpatients with significant hyperglycaemia were treated and compared with 45 historical controls (with similar age, HbA1c and diabetes duration) treated with sliding scale insulin (SSI).The BGL (mean±SD) was significantly lower during BBB treatment (11.7±2.6 vs 13.6±2.4mmol/L; p<0.0001), while episodes of hyperglycaemia were less (median: 3 [IQR 1–8] vs 7 [IQR 4–12]; p=0.001). Patients who experienced hypoglycaemia were also less likely to have a repeat episode with the BBB protocol (median: 1 [IQR 1–3] vs 3 [IQR 2–4.5]).The BBB protocol is easy to implement and resulted in significant improvement in BGL control compared with SSI. Copyright © 2011 John Wiley & Sons.
Proteins linked to extinction in contextual fear conditioning in the C57BL/6J mouse
Authors: Li Lin , Boddul Sanjay V. , Patil Sudarshan S. , Zheng Jun‐Fang , An Gunyong , Höger Harald , Lubec Gert 
Published: July 2011

AbstractStudying fear extinction is a major topic in neuroscience. No information on systematic studies on the linkage of contextual fear conditioning (cFC) with hippocampal protein levels is available and we were therefore interested in protein differences between animals with poor and good extinction. cFC was carried out in C57BL/6J mice, hippocampi were taken and proteins were run on two‐dimensional gel electrophoresis with subsequent quantification of protein spots. In‐gel digestion with trypsin and identification by ion trap MS/MS (high‐capacity ion trap) was used for the identification of significantly different hippocampal proteins between mice with good and poor performance of extinction. Signaling protein ras‐related protein rab‐7A and septin 8 levels were significantly higher in hippocampus of poor extinguishers, whereas ubiquitin carboxyterminal hydrolase isozyme L1 showed higher levels in animals with good extinction performance. A series of additional proteins showed significantly different levels between groups but the abovementioned were confirmed by immunoblotting. The abovementioned proteins have never been reported to be linked to extinction, memory, or learning and herein evidence for the involvement of several proteins in extinction mechanism as well as probably representing pharmaceutical targets is provided. Moreover, it is intriguing to demonstrate the differences between good and poor extinction performance at the protein level.
An enhanced chemically defined SILAC culture system for quantitative proteomics study of human embryonic stem cells
Authors: Wang Shuai , Tian Ruijun , Li Li , Figeys Daniel , Wang Lisheng 
Published: July 2011

AbstractStable isotope labeling by SILAC‐based quantitative proteomics analysis provides an unprecedented tool for the study of mechanisms underlying the self‐renewal and differentiation of human embryonic stem cells (hESCs). While we recently reported a chemically defined SILAC culture system specific for a rare cell proteomic reactor (R. Tian et al., Mol. Cell. Proteomics 2011, 10, M110.000679), total hESC yield, prolonged self‐renewal capacity (i.e.<12 days), and laborious procedure remain substantial hurdles for its conventional application in hESC studies. Here, we devised an enhanced SILAC culture system consisting of a new chemically defined SILAC‐medium and a novel culture protocol. As a result, with much less culture maneuvers, approximately 40‐fold greater hESCs were produced than the system reported previously. Moreover, the enhanced SILAC culture system was sufficient to support the self‐renewal of hESCs for >60 days and was also highly reproducible. As such, it provides a new platform that can be readily adapted by general laboratory for further comprehensive SILAC‐based proteomics analysis of hESCs and induced pluripotent stem cells.
Temporal proteomic profiling of embryonic stem cell secretome during cardiac and neural differentiation
Authors: Farina Annarita , D'Aniello Cristina , Severino Valeria , Hochstrasser Denis F. , Parente Augusto , Minchiotti Gabriella , Chambery Angela 
Published: July 2011

AbstractDuring recent years, increased efforts have focused on elucidating the pluripotency and self‐renewal of stem cells. Differentiation towards the different lineages has attracted significant attention given the potential use of stem cells in regenerative medicine. Embryonic stem cell differentiation is a complex process coordinated by strictly regulated extracellular signals that act in an autocrine and/or paracrine manner. Through secreted molecules, stem cells affect local niche biology and influence the cross‐talking with the surrounding tissues. Emerging evidence supports the hypothesis that fundamental cell functions, including proliferation and differentiation, are strictly regulated by the complex set of molecules secreted from cells. The understanding of this molecular language could largely increase our knowledge on pathways regulating stem cell differentiation. Here, we have used a proteomics platform to investigate the profile of proteins secreted during differentiation of murine embryonic stem cells. We have followed the dynamics of protein secretion by comparing the secretomes at different time points of murine embryonic stem cell cardiac and neural differentiation. In addition to previously reported molecules, we have identified many secreted proteins not described so far as released from embryonic stem cells nor shown to be differentially released during the process of cardiomyogenesis and neurogenesis.