bluuebunny/crossref_metadata_2025_split huggingface dataset:
STIM1, ORAI1, and KDM2B in circulating tumor cells (CTCs) isolated from prostate cancer patients
Authors: Roumeliotou Argyro , Alkahtani Saad , Alarifi Saud , Alkahtane Abdullah A. , Stournaras Christos , Kallergi Galatea 
Published: June 2024

Introduction: Previous publications have shown that STIM1, ORAI1, and KDM2B, are implicated in Ca2+ signaling and are highly expressed in various cancer subtypes including prostate cancer. They play multiple roles in cancer cell migration, invasion, and metastasis. In the current study we investigated the expression of the above biomarkers in circulating tumor cells from patients with metastatic prostate cancer.Methods: Thirty-two patients were enrolled in this study and CTCs’ isolation was performed with Ficoll density gradient. Two different triple immunofluorescence stainings were conducted with the following combination of antibodies: CK/KDM2B/CD45 and CK/STIM1/ORAI1. Slides were analyzed using VyCAP microscopy technology.Results: CTC-positive patients were detected in 41% for (CK/KDM2B/CD45) staining and in 56% for (CK/STIM1/ORAI1) staining. The (CK+/KDM2B+/CD45–) and the (CK+/STIM1+/ORAI1+) were the most frequent phenotypes as they were detected in 85% and 94% of the CTC-positive patients, respectively. Furthermore, the expression of ORAI1 and STIM1 in patients’ PBMCs was very low exhibiting them as interesting specific biomarkers for CTC detection. The (CK+/STIM1+/ORAI1+) phenotype was correlated to bone metastasis (p = 0.034), while the (CK+/STIM1+/ORAI1–) to disease relapse (p = 0.049).Discussion: STIM1, ORAI1, and KDM2B were overexpressed in CTCs from patients with metastatic prostate cancer. STIM1 and ORAI1 expression was related to disease recurrence and bone metastasis. Further investigation of these biomarkers in a larger cohort of patients will clarify their clinical significance for prostate cancer patients.
Exploring the effects of real-time online cardiac telerehabilitation using wearable devices compared to gym-based cardiac exercise in people with a recent myocardial infarction: a randomised controlled trial
Authors: Mitropoulos A. , Anifanti M. , Koukouvou G. , Ntovoli A. , Alexandris K. , Kouidi E. 
Published: June 2024

BackgroundExercise-based cardiac rehabilitation (CR) is a non-pharmacological multidisciplinary programme for individuals after myocardial infarction (MI) that offers multiple health benefits. One of the greatest barriers to CR participation is the travel distance to the rehabilitation centre. Remotely monitored CR appears to be at least as effective in improving cardiovascular risk factors and exercise capacity as traditional centre-based CR. Nevertheless, the efficacy of remotely monitored CR in individuals with a recent MI has yet to be examined.MethodsA total of 30 individuals (8 women, 22 men) after a recent (i.e., &lt;4 weeks) MI were randomly allocated into two groups (online home-based and gym-based groups). Both groups underwent a 26-week CR programme three times per week. All patients performed baseline and 24-week follow-up measurements where peak oxygen uptake (VO2peak), mean daily steps, distance, and calories were assessed.ResultsThe online group showed an improvement in mean daily steps (p &lt; 0.05) and mean daily distance (p &lt; 0.05) at 24 weeks compared to the gym-based group. The paired-sample t-test showed that all the assessed variables were statistically (p &lt; 0.001) improved for both groups at 24 weeks. Pearson's r demonstrated positive correlations between VO2peak and mean daily distance (r = 0.375), and negative correlations between VO2peak and muscle (r = −0.523) and fat masses (r = −0.460). There were no exercise-induced adverse events during the study.ConclusionOur findings might indicate that a real-time online supervised CR exercise programme using wearable technology to monitor the haemodynamic responses in post-MI patients is equally effective as a gym-based exercise programme.
Staying connected: implementing avatar robots at schools in Germany and Japan
Authors: Spoden Celia , Ema Arisa 
Published: June 2024

IntroductionWith advancements in communication technologies and internet connectivity, avatar robots for children who cannot attend school in person due to illness or disabilities have become more widespread. Introducing these technologies to the classroom aims to offer possibilities of social and educational inclusion. While implementation is still at an experimental level, several of these avatars have already been introduced as a marketable service. However, various obstacles impede widespread acceptance.MethodsIn our explorative qualitative case study we conducted semi-structured interviews with eight individuals involved in the implementation of the avatar robots AV1 in Germany and eleven participants involved with implementing OriHime in Japan. We analyzed and compared implementation processes, application areas, access and eligibility, and the potential and limitations of avatars at schools.ResultsWe identified structural similarities and differences in both countries. In the German cases the target is defined as temporary use for children who cannot attend school in person because of childhood illness, with the clear goal of returning to school. Whereas in Japan OriHime is also implemented for children with physical or developmental disabilities, or who cannot attend school in person for other reasons.DiscussionOur study suggests that avatar technologies bear high potential for children to stay socially and educationally connected. Yet, structures need establishing that grant equal access to avatar technologies. These include educational board regulations, budgets for funding avatar technologies and making them accessible to the public, and privacy protection standards that are adequate, yet do not create implementation hurdles that are too high. Furthermore, guidelines or training sessions on technical, educational and psychosocial aspects of including avatar technologies in the classroom for teachers are important for successful implementation. Since our Japanese cases suggest that expanding the area of application beyond childhood illness is promising, further research on the benefits for different groups is needed.
A CFD numerical simulation of particle deposition characteristics in automobile tailpipe: power abatement pathways
Authors: Zhang Rui , Gao Ming , Fan Mao 
Published: June 2024

The study of the movement of pollutants through ducts facilitates the assessment and control of ambient air quality problems (AQ). Among other things, understanding the deposition and distribution of particulate matter in elbows is important for practical engineering applications. In this study, the turbulent flow field and particle deposition in a 90° bend is investigated using RANS simulation. The RNG k-ε turbulence model was employed to calculate the airflow flow field and the discrete phase model (DPM) was used to simulate the particle phase motion. Where for the discrete phase, the Discrete Random Wander (DRW) model was considered and the deposition of particles with sizes of 1, 3, 5, 10, 20, and 40 μm in the flow field was investigated separately. Grid-independent validation of the models used in the simulations was performed. The effects of inlet velocity, particle size, and direction of gravity on the flow field and particle deposition in the elbow were considered. The results show that the flow field in the bend is strongly influenced by the above parameters. Among them, the turbulent disturbance in the bend section is the most intense, with high turbulent energy value, and it is also the region with the largest energy loss. The inlet velocity is negatively correlated with the deposition rate, and the particle size is positively correlated with the deposition rate.
The barrier risk to the ecological connectivity of plant diversity in karst landscapes in Guizhou Province, China
Authors: Zhou Baichi , Lou Hezhen , Yang Shengtian , Li Chaojun , Pan Zihao , Zhang Yujia , Li Hao , Yi Yin , Gong Jiyi 
Published: June 2024

Ecological connectivity in landscapes is crucial for plant diversity conservation. The barrier risk to ecological connectivity represents the risk to ecological connectivity loss or weakening, resulting from the barrier to biological information exchange among habitats. Therefore, clarifying the barrier risk to the ecological connectivity of plant diversity in space can reveal the spatial impacts of reduced ecological connectivity on plant diversity. This study analyzed effects of karst peak, river network, arable land, and impervious surface on plant diversity in karst natural, countryside, urban, and island landscapes in Guizhou Province with fragile environment. Then, we calculated the barrier distance of ecological connectivity to reveal the barrier risk to the ecological connectivity of plant diversity in space. The results showed that karst peak was the source of high plant diversity, and plant diversity could diffuse about 400 m around karst peaks. River network and arable land enhanced the connectivity among karst peaks to maintain plant diversity, and the effect on enhancing the connectivity was about 300 m and 450 m, respectively, while the weakening effect of impervious surface on connectivity was about 350 m. Based on the distance for plant diversity diffusing around karst peaks, the barrier distance of ecological connectivity was determined by the combination type of river network, arable land and impervious surface in landscapes. From low to high, the barrier risk to the ecological connectivity of plant diversity was about 1,110 m in the combination of river network and arable land, about 790 m in the combination of river network, arable land and impervious surface, about 520 min the combination of arable land and impervious surface, about 400 m in the combination of river network and impervious surface. Our findings clarify the barrier risk to the ecological connectivity of plant diversity in space, and provide a scientific basis for plant diversity conservation from the perspective of ecological connectivity.
Constructing ecological security pattern based on spatio-temporal evaluation of ecosystem services and ecological health in Chengdu, Southwest China
Authors: Zhong Bo , Wu Shuang , Wu Ning , Sun Geng , He Chunmei , Liu Liangfeng , Chen Huai 
Published: June 2024

IntroductionWith the acceleration of urbanization, human population and built surface in urban areas have increased rapidly, triggering numerous environmental problems. Identification of ecological security pattern (ESP) can be helpful to optimize the interaction and relationship between ecological conservation and socioeconomic development in a given region. In this study, taking Chengdu City as an example, a methodology was used to analyze the city’s ESP.MethodologyEcological sources were identified based on their ecological security values, which were derived from the spatio-temporal evaluation of ecosystem services and ecological health. The ecological resistance surface was revised with nighttime light index. Linkage Mapper combined with circuit theory was used to extracted ecological corridors and key ecological nodes.Results:The results showed that the mean values of ecological security in Chengdu City presented a downward and then upward trend from 2000 to 2018. In 2018, the ESP in Chengdu City was formed by 140 ecological sources with the total area of 8,819.78 km2, 302 ecological corridors with the area of 456.91 km2, as well as 61 pinch-points and 17 barrier points. The ecological sources in Chengdu City were mainly distributed in or nearby Longmen Mountain and Longquan Mountain, two flanks of the Chengdu Plain, and connected each other with hundreds of ecological corridors. Most of ecological sources and corridors were composited of forests in mountainous areas and linpan units (wooded lots) in rural plain areas. On the contrary, pinch-points and barrier points were mainly distributed in the districts nearby the metropolitan urban center.ConclusionFinally, this study proposed that constructing ESP must adopt a dynamic and holistic approach in considering the change of restoring ecosystems, shift of urban demands for ecosystem services and transformation of urban-rural land use/cover. Restoring and managing corridors sustainably are important for improving the ecological connectivity of all over ecological sources in a city, all of which together, if functioning well, could further support the realization of sustainable urbanization.
MicroRNA-939 amplifies Staphylococcus aureus-induced matrix metalloproteinase expression in atopic dermatitis
Authors: Wang Jiating , Huang Yejing , Wu Xinfeng , Li Dongqing 
Published: June 2024

BackgroundAtopic dermatitis (AD) is a common chronic inflammatory skin diseases that seriously affects life quality of the patients. Staphylococcus aureus (S. aureus) colonization on the skin plays an important role in the pathogenesis of AD; however, the mechanism of how it modulates skin immunity to exacerbate AD remains unclear. MicroRNAs are short non-coding RNAs that act as post-transcriptional regulators of genes. They are involved in the pathogenesis of various inflammatory skin diseases.MethodsIn this study, we established miRNA expression profiles for keratinocytes stimulated with heat-killed S. aureus (HKSA). The expression of miR-939 in atopic dermatitis patients was analyzed by fluorescence in situ hybridization (FISH). miR-939 mimic was transfected to human primary keratinocyte to investigate its impact on the expression of matrix metalloproteinase genes (MMPs) in vitro. Subsequently, miR-939, along with Polyplus transfection reagent, was administered to MC903-induced atopic dermatitis skin to assess its function in vivo.ResultsMiR-939 was highly upregulated in HKSA-stimulated keratinocytes and AD lesions. In vitro studies revealed that miR-939 increased the expression of matrix metalloproteinase genes, including MMP1, MMP3, and MMP9, as well as the cell adhesion molecule ICAM1 in human primary keratinocytes. In vivo studies indicated that miR-939 increased the expression of matrix metalloproteinases to promote the colonization of S. aureus and exacerbated S. aureus-induced AD-like skin inflammation.ConclusionsOur work reveals miR-939 is an important regulator of skin inflammation in AD that could be used as a potential therapeutic target for AD.
Dynamic immune status analysis of peripheral blood mononuclear cells in patients with Klebsiella pneumoniae bloodstream infection sepsis using single-cell RNA sequencing
Authors: Zhang Shengwei , Zhang Nan , Han Jing , Sun Zeyu , Jiang Hua , Huang Wenhua , Kong Decong , Li Qian , Ren Yuhao , Zhao Shishun , Jiang Yongqiang , Liu Peng 
Published: June 2024

BackgroundKlebsiella pneumoniae is a common Gram-negative bacterium. Blood infection caused by K. pneumoniae is one of the most common causes of human sepsis, which seriously threatens the life of patients. The immune status of peripheral blood mononuclear cells (PBMCs) based on single-cell RNA sequencing (scRNA-seq) in acute stage and recovery stage of sepsis caused by K. pneumoniae bloodstream infection has not been studied.MethodsA total of 13 subjects were included in this study, 3 healthy controls, 7 patients with K. pneumoniae bloodstream infection in the acute stage (4 patients died), and 3 patients in the recovery stage. Peripheral blood of all patients was collected and PBMCs were isolated for scRNA-seq analysis. We studied the changes of PBMCs components, signaling pathways, differential genes, and cytokines in acute and recovery stages.ResultsDuring K. pneumoniae acute infection we observed a decrease in the proportion of T cells, most probably due to apoptosis and the function of T cell subtypes was disorder. The proportion of monocytes increased in acute stage. Although genes related to their phagocytosis function were upregulated, their antigen presentation capacity-associated genes were downregulated. The expression of IL-1β, IL-18, IFNGR1 and IFNGR2 genes was also increased in monocytes. The proportion of DCs was depleted during the acute stage and did not recover during sepsis recovery. DCs antigen presentation was weakened during the acute stage but recovered fast during the recovery stage. pDCs response to MCP-1 chemokine was weakened, they recovered it quickly during the recovery stage. B cells showed apoptosis both in the acute stage and recovery stage. Their response to complement was weakened, but their antigen presentation function was enhanced. The proportion of NK cells stable during all disease’s stages, and the expression of IFN-γ gene was upregulated.ConclusionThe proportion of PBMCs and their immune functions undergo variations throughout the course of the disease, spanning from the acute stage to recovery. These findings provide new insights into the mechanism of PBMCs immune function during K. pneumoniae bloodstream infection sepsis and recovery and sets the basis for further understanding and treatment.
Evaluation of the probiotic, technological, safety attributes, and GABA-producing capacity of microorganisms isolated from Iranian milk kefir beverages
Authors: Moghimani Minoo , Onyeaka Helen , Hashemi Mohammad , Afshari Asma 
Published: June 2024

IntroductionKefir beverage has beneficial microorganisms that have health-giving properties; therefore, they have a good potential to be probiotic. This study evaluated the probiotic potential, technological, and safety characteristics of Enterococcus faecalis, Lactococcus lactis, and Pichia fermentans isolated from traditional kefir beverages.MethodFirst, isolates were evaluated in terms of resistance to acid, alkali, bile salts, trypsin, and pepsin of the gastrointestinal tract. The auto-aggregation and co-aggregation ability of isolates were measured using spectrophotometry. Antimicrobial activities were assayed against important food-borne pathogens using the agar well diffusion method. Moreover, gamma-aminobutyric acid (GABA) production was investigated by thin-layer chromatography (TLC).ResultAmong the isolates, P. fermentans had an 85% total survival rate, but its amount reached below 6 log CFU/ml which is considered non-resistant, and it showed the highest auto-aggregation (74.67%). Moreover, only L. lactis showed antimicrobial activity and had the highest co-aggregation with E. coli PTCC 1338 (54.33%) and L. monocytogenes ATCC 7644 (78%). Finally, an evaluation of the technological and safety characteristics of the strains showed that the strains produced GABA and were safe.DiscussionAlthough the isolates were not resistant to the gastrointestinal tract, their supernatant contained valuable natural compounds, including antioxidants, GABA, and antimicrobials, which can be used to produce functional foods and medicines. In addition, other approaches, such as increasing the initial number of strains, using foods as carriers of isolates, and encapsulating the isolates, can effectively increase the survivability of isolates in the gastrointestinal tract.
The peptidoglycan-associated lipoprotein gene mutant elicits robust immunological defense in mice against Salmonella enteritidis
Authors: Zhao Guixin , Duan Wenlong , Zhang Lu , Sun Wenchao , Liu Wan , Zhang Xiaoyu , Zhang Yanying , Shi Qiumei , Wu Tonglei 
Published: June 2024

BackgroundSalmonella enteritidis (S. enteritidis), a zoonotic pathogen with a broad host range, presents a substantial threat to global public health safety. Vaccination stands as an effective strategy for the prevention and control of S. enteritidis infection, highlighting an immediate clinical need for the creation of safe and efficient attenuated live vaccines.MethodsIn this study, a S. enteritidis peptidoglycan-associated lipoprotein (pal) gene deletion strain (Δpal), was constructed. To assess its virulence, we conducted experiments on biofilm formation capability, motility, as well as cell and mouse infection. Subsequently, we evaluated the immune-protective effect of Δpal.ResultsIt was discovered that deletion of the pal gene reduced the biofilm formation capability and motility of S. enteritidis. Cell infection experiments revealed that the Δpal strain exhibited significantly decreased abilities in invasion, adhesion, and intracellular survival, with downregulation of virulence gene expression, including mgtC, invH, spvB, sipA, sipB, ssaV, csgA, and pipB. Mouse infection experiments showed that the LD50 of Δpal increased by 104 times, and its colonization ability in mouse tissue organs was significantly reduced. The results indicated that the pal gene severely affected the virulence of S. enteritidis. Further, immunogenicity evaluation of Δpal showed a significant enhancement in the lymphocyte transformation proliferation capability of immunized mice, producing high titers of specific IgG and IgA, suggesting that Δpal possesses good immunogenicity. Challenge protection tests demonstrated that the strain could provide 100% immune protection against wild-type strains in mice.DiscussionThis study proves that the pal gene influences the virulence of S. enteritidis, and Δpal could serve as a candidate strain for attenuated live vaccines, laying the foundation for the development of attenuated live vaccines against Salmonella.
Cathepsins and Parkinson’s disease: insights from Mendelian randomization analyses
Authors: Yusufujiang Aishanjiang , Zeng Shan , Li Hongyan 
Published: June 2024

BackgroundParkinson’s disease (PD), the second most prevalent neurodegenerative condition, has a multifaceted etiology. Cathepsin-cysteine proteases situated within lysosomes participate in a range of physiological and pathological processes, including the degradation of harmful proteins. Prior research has pointed towards a potential link between cathepsins and PD; however, the precise causal relationship between the cathepsin family and PD remains unclear.MethodsThis study employed univariate and multivariate Mendelian randomization (MR) analyses to explore the causal relationship between the nine cathepsins and Parkinson’s disease (PD) risk. For the primary analysis, genome-wide association study (GWAS) summary statistics for the plasma levels of the nine cathepsins and PD was obtained from the INTERVAL study and the International Parkinson’s Disease Genomics Consortium. GWAS for PD replication analysis were obtained from the FinnGen consortium, and a meta-analysis was performed for the primary and replication analyses to evaluate the association between genetically predicted cathepsin plasma levels and PD risk. After identifying significant MR estimates, genetic co-localization analyses were conducted to determine whether shared or distinct causal variants influenced both cathepsins and PD.ResultsElevated cathepsin B levels were associated with a decreased risk of PD in univariate MR analysis (odds ratio [OR] = 0.890, 95% confidence interval [CI]: 0.831–0.954, pFDR = 0.009). However, there was no indication that PD affected cathepsin B levels (OR = 0.965, 95% CI: 0.858–1.087, p = 0.852). In addition, after adjusting for the remaining cathepsins, cathepsin B levels independently and significantly contributed to the reduced risk of PD in multivariate MR analysis (OR = 0.887, 95% CI: 0.823–0.957, p = 0.002). The results of the replication MR analysis with the FinnGen GWAS for PD (OR = 0.921, 95% CI: 0.860–0.987, p = 0.020) and meta-analysis (OR = 0.905, 95% CI: 0.862–0.951, p &lt; 0.001) were consistent with those of the primary analysis. Colocalization analysis did not provide any evidence of a shared causal variant between cathepsins and PD (PP.H4.abf = 0.005).ConclusionThis genetic investigation supports the hypothesis that cathepsin B exerts a protective effect against PD. The quantification of cathepsin B levels could potentially serve as a predictive biomarker for susceptibility to PD, providing new insights into the pathomechanisms of the disease and possible interventions.