bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interactive software application for calculation of non‐stationary heat conduction in a cylindrical body
Authors: Janáčová Dagmar , Charvátová Hana , Kolomazník Karel , Fialka Miloslav , Mokrejš Pavel , Vašek Vladimír 
Published: June 2010

AbstractIn this paper we present software application used for the study of heat transfer problems that is a part of education of subject Process Engineering taught at the Tomas Bata University in Zlín. The application speeds up calculation of non‐stationary heat conduction and visualizes temperature distribution in the cylindrical body. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 21: 89–94, 2013
DSP‐based real‐time platform for remote control of internet‐connected systems
Authors: Peñaloza‐Mejía O. , Márquez‐Martínez L. A. , Alvarez‐Gallegos Ja. , Estrada‐García H. J. 
Published: June 2010

AbstractThis work presents the development of an experimental platform for remote control of Internet‐connected systems. The aim is to describe and to evaluate this platform, which exhibits some interesting peculiarities w.r.t. the mainstream literature: real‐time support over the Internet, capacity to handle loss/disorder of packets, and varying delays. Practical tests are given to show its capabilities to telecontrol different systems. The evaluation is carried out comparing the performance of some controllers for position coordination of a pair of local–remote Internet‐connected systems. Besides, an assessment of platform usage and learning effectiveness was developed to conclude on its pedagogical viability in engineering education. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 21: 203–213, 2013
Site‐Directed Mutagenesis of Rice Serine Racemase: Evidence That Glu219 and Asp225 Mediate the Effects of Mg2+ on the Activity
Authors: Gogami Yoshitaka , Kobayashi Ai , Ikeuchi Toshihiko , Oikawa Tadao 
Published: June 2010

AbstractWe succeeded in constructing the Glu219Ala/Asp225Ala (i.e., E219A/D225A) serine racemase (SerR) by site‐directed mutagenesis, and the effects of Mg2+ on the catalytic efficiency and the structure were compared between the E219A/D225A‐SerR and the wild‐type protein. This is the first example of a serine racemase whose amino acid residues in the Mg2+‐binding site were replaced with other amino acids by site‐directed mutagenesis. Neither the serine racemase nor the dehydratase activities of the E219A/D225A‐SerR were affected by the addition of Mg2+, and Glu219 and Asp225 of the SerR are the essential amino acid residues for Mg2+ to affect both kinds of enzyme activities. Therefore, Glu219 and Asp225 mediate the effects of Mg2+ on the activity and are important for the SerR to form the Mg2+‐binding site. Judging from the difference of the Keq values between the E219A/D225A‐SerR and the SerR, Mg2+ might affect the equilibrium states in the racemase reaction. The fluorescence quenching analysis of the E219A/D225A‐SerR showed that Mg2+ bound to Glu219 and Asp225 of the SerR probably causes a conformational change in the ternary structure of the SerR.
Detection and Function of the Intramolecular Disulfide Bond in Arginine Racemase: An Enzyme with Broad Substrate Specificity
Authors: Matsui Daisuke , Oikawa Tadao 
Published: June 2010

AbstractWe found that a single intramolecular disulfide bond between the cysteines C47 and C73 exists in the primary structure of arginine racemase (ArgR) from Pseudomonas taetrolens NBRC 3460, and this is the first example of a pyridoxal phosphate (PLP)‐dependent amino acid racemase that contains a disulfide bond. The amino acid racemase activity was still detected, when the disulfide bond of ArgR was disrupted by site‐directed mutagenesis or reduced with dithiothreitol (DTT). The thermal and pH profiles and the quaternary structure of ArgR did not change when the disulfide bond of ArgR was disrupted by site‐directed mutagenesis. The substrate specificity and the overall structure did not change when the disulfide bond of ArgR was reduced with DTT after the protein was matured. However, these properties changed when the disulfide bond of ArgR was disrupted by site‐directed mutagenesis before protein maturation. The total activity of ArgR decreased when the disulfide bond of ArgR was disrupted by site‐directed mutagenesis before the protein was matured or when ArgR was expressed in the cytoplasm. Based on these results, we can conclude that the disulfide bond of ArgR is essential for ArgR to fold and mature as an amino acid racemase with broad substrate specificity.
Indispensable but Insufficient Role of RenalD‐Amino Acid Oxidase in Chiral Inversion ofNG‐Nitro‐D‐arginine
Authors: Xin Yan‐Fei , Li Xin , Hao Bin , Gong Nian , Sun Wen‐Qiang , Konno Ryuichi , Wang Yong‐Xiang 
Published: June 2010

AbstractUnidirectionally chiral inversion ofNG‐nitro‐D‐arginine (D‐NNA) to itsL‐enantiomer (L‐NNA) occurred in rats, and it was blocked markedly (ca.80%) by renal vascular ligation, and entirely (100%) by theD‐amino acid oxidase (DAO) inhibitor sodium benzoate, suggesting that renal DAO is essential for the inversion. However, the doses of sodium benzoate administrated were extremely high (e.g., 400 mg/kg) due to its low potency. It is thus possible that sodium benzoate‐mediated blockade ofD‐NNA inversion might be due to its nonspecific (or non‐DAO‐related) effects. In addition, afterD‐NNA was incubated with the pure enzyme of DAOin vitrowithout tissue homogenates,L‐NNA was not produced, even thoughD‐NNA was disposed. We propose that this occurred becauseD‐NNA was first converted to its correspondingα‐keto acid by DAO and then toL‐NNA by transaminase(s); however, there was no direct evidence for this process. The goal of this study is to further elucidate the process ofD‐NNA chiral inversion bothin vivoand inin vitrotissue homogenates by comparing mutant ddY/DAO−/−mice that lack DAO activity entirely compared to normal ddY/DAO+/+mice and Swiss mice. Furthermore, the ability to produceL‐NNA fromD‐NNA‐correspondingα‐keto acids (NG‐nitroguanidino‐2‐oxopentanoic acid) produced by porcine kidney‐derived DAO (pkDAO) was also studied in the DAO inhibitor‐pretreated rats. We found thatD‐NNA chiral inversion occurred in Swiss mice and ddY/DAO+/+mice bothin vivoand inin vitrokidney homogenates, but not in ddY/DAO−/−mice, correlated to their DAO activities. Theα‐keto acid (NG‐nitro‐guanidino‐2‐oxopentanoic acid) fromD‐NNA was able to produceL‐NNA, and subsequent vasoconstriction and pressor responses. These results indicate that the role of renal DAO is indispensible but insufficient for chiral inversion ofD‐NNA and other neutral and polarD‐amino acids, and unidentified aminotransferase(s) are involved in a subsequent mechanism for the process of chiral inversion.
Comparative Characterization of Three D‐Aspartate Oxidases and One D‐Amino Acid Oxidase from Caenorhabditis elegans
Authors: Katane Masumi , Saitoh Yasuaki , Seida Yousuke , Sekine Masae , Furuchi Takemitsu , Homma Hiroshi 
Published: June 2010

AbstractPreviously, we cloned cDNAs for four Caenorhabditis elegans genes (F20 Hp, C47Ap, F18Ep, and Y69Ap genes) that were annotated in the database as encoding D‐amino acid oxidase (DAO) or D‐aspartate oxidase (DDO) proteins. These genes were expressed in Escherichia coli, and the recombinant C47Ap and F18Ep were shown to have functional DDO activities, while Y69Ap had functional DAO activity. In this study, we improved the E. coli culture conditions for the production of recombinant F20 Hp and, following purification of the protein, revealed that it has functional DDO activity. The kinetic properties of recombinant C47Ap (DDO‐1), F18Ep (DDO‐2), F20 Hp (DDO‐3), and Y69Ap (DAO) were also determined and compared with recombinant human DDO and DAO. In contrast to the low catalytic efficiency of human DDO for D‐Glu, all three C. elegans DDOs showed higher catalytic efficiencies for D‐Glu than D‐Asp or N‐methyl‐D‐Asp. The catalytic efficiency of C. elegans DAO for D‐Ser was substantially lower than that of human DAO, while the C. elegans DAO was more efficient at deamination of basic D‐amino acids (D‐Arg and D‐His) than human DAO. Collectively, our results indicate that C. elegans contains at least three genes that encode functional DDOs, and one gene encoding a functional DAO, and that these enzymes have different and distinctive properties.
Computational Insight into the Mechanism of Serine Residue Racemization
Authors: Takahashi Ohgi , Kobayashi Kana , Oda Akifumi 
Published: June 2010

AbstractDespite the known racemization of serine (Ser) residues in proteins from aged or diseased human brains, the mechanism of Ser racemization in peptides and proteins has not been studied. This is in contrast to the case of the rapid racemization of aspartic acid (Asp) residues, for which a succinimide‐mediated mechanism is established. In a possible mechanism for Ser racemization, the enolization involving the H(α)‐atom and the CO(α) group occurs by active participation of two H2O molecules. In this study, we computationally modeled this two‐H2O mechanism of enolization for Ser and alanine (Ala) residues using model compounds, and the results were compared with each other and with the case of succinimide analogues reported in a separated paper. Our results suggest that Ser residues are much more racemization‐prone than Ala residues, but to a lesser extent than Asp residues. The side‐chain CO bond of the Ser residue stabilizes the enolization transition state hyperconjugatively.
Aberrant Control of Motoneuronal Excitability in Amyotrophic Lateral Sclerosis: Excitatory Glutamate / D‐Serinevs.Inhibitory Glycine/γ‐Aminobutanoic Acid (GABA)
Authors: Sasabe Jumpei , Aiso Sadakazu 
Published: June 2010

AbstractThe mechanism underlying selective motoneuronal loss in amyotrophic lateral sclerosis (ALS) remains uncertain. The pathogenesis appears to be a complex and multifactorial process. Glutamate excitotoxicity to motoneuron is one of the most intensely investigated targets for the treatment of ALS, and excessive motoneuronal excitation by glutamate through ionotropic glutamate receptors has been mainly demonstrated. However, development of clinically effective drug targeting glutamate is sometimes difficult, because some aspects of glutamergic signals also could be beneficial, as the injured neurons attempt to recruit endogenous recovery. This review is focused on identifying other mechanisms of imbalanced excitation in ALS motoneurons including excitation‐modulatingD‐serine and inhibitory glycine/GABA. Further, validation of these mechanisms might ultimately lead us to new therapeutic targets for ALS.
Accumulation of D‐β‐Aspartic Acid‐Containing Proteins in Age‐Related Ocular Diseases
Authors: Kaji Yuichi , Oshika Tetsuro , Takazawa Yutaka , Fukayama Masashi , Fujii Noriko 
Published: June 2010

AbstractTransparency is essential for eyes, which serve as cameras. However, decreased transparency caused by accumulation of abnormal proteins is commonly observed especially in elderly people. However, the mechanism of accumulation of abnormal proteins in various ocular diseases is still unclear. In the present study, we investigated the role of D‐amino acids in the development of accumulation of abnormal proteins in age‐related ocular diseases.Whole eyes with age‐related macular degeneration were excised from four patients. Surgical specimens with pinguecula were excised from eight patients, and specimens with climatic droplet keratopathy were excised from three patients. Polyclonal antibody to the D‐β‐Asp‐containing peptide was prepared. Using the antibody, immunohistochemical localization of D‐β‐Asp‐containing proteins was determined.In eyes with age‐related macular degeneration, accumulation of abnormal proteins was observed between the retinal pigment epithelial cells and Bruch's membrane. The accumulated abnormal proteins were positive for D‐β‐Asp‐containing peptides. In surgical specimens with pinguecula and climatic droplet keratopathy, abnormal accumulation of proteins seen in the superficial layer of the stroma was positive for D‐β‐Asp‐containing peptide.The results indicate that development of D‐β‐Asp‐containing proteins in vivo have a central role in the development of accumulation of abnormal proteins in age‐related macular degeneration, pinguecula, and climatic drop‐like dystrophy.
Quantification of Structural Alterations of L‐Asp and L‐Asn Residues in Peptides Related to Neuronal Diseases by Reversed‐Phase High‐Performance Liquid Chromatography
Authors: Sadakane Yutaka , Konoha Keiko , Kawahara Masahiro , Nakagomi Kazuya 
Published: June 2010

AbstractA method for analyzing the structural alterations in Asn or Asp residues was developed by using the peptides related to neuronal conformational diseases, i.e., the prion protein (PrP)106–126 and the Alzheimer's amyloid β (Aβ) protein6–28. The alterations were analyzed by reversed‐phase (RP) HPLC, because the peptides containing the structurally altered residues were diastereoisomers of each other, and they were separated with the mobile phase containing an MeCN/sodium phosphate solution and NaCl. The amount of L‐Asp, L‐isoAsp, D‐Asp, or D‐isoAsp residues in each PrP peptide isomer was simultaneously quantified by carrying out single HPLC analysis; these residues were generated by the deamidation of the Asn residue. Only 0.3% of the newly generated peptide containing the D‐Asp residue was detected. Furthermore, the investigation of the partial fragment of the Aβ protein revealed that the present method possessed the ability of simultaneous analysis of the isomerizations of two Asp residues. These results implied that the present method was highly sensitive and reduced the time required for the analysis. This method may accelerate the elucidation of the PrP and Aβ protein functions, because the structural alterations of Asn and Asp have been reported to influence these functions.
Effects of D‐Aspartate Treatment on D‐Aspartate Oxidase, Superoxide Dismutase, and Caspase 3 Activities in Frog (Rana esculenta) Tissues
Authors: Burrone Lavinia , Di Giovanni Marcello , Di Fiore M. Maddalena , Chieffi Baccari Gabriella , Santillo Alessandra 
Published: June 2010

AbstractAlthough D‐aspartate (D‐Asp) has been recognized to have a physiological role within different organs, high concentrations could elicit detrimental effects on those same organs. In this study, we examined the D‐aspartate oxidase (D‐AspO) activity and the expression of superoxide dismutase 1 (SOD1) and caspase 3 in different tissues of the frog Rana esculenta after chronic D‐Asp treatment. Our in vivo experiments, consisting of intraperitoneal (ip) injections of D‐Asp (2.0 μmol/g b.w.) in frogs for ten consecutive days, revealed that all examined tissues can take up and accumulate D‐Asp. Further, in D‐Asp treated frogs, i) the D‐AspO activity significantly increased in all tissues (kidney, heart, testis, liver, and brain), ii) the SOD1 expression (antioxidant enzyme) significantly increased in the kidney, and iii) the caspase 3 level (indicative of apoptosis) increased in both brain and heart. Particularly, after the D‐Asp treatment we found in both brain and heart (which showed the lowest SOD1 levels) a significant increase of the caspase 3 expression and, vice versa, in the kidney (which showed the highest SOD1 expression) a significant decrease of the caspase 3 expression. Therefore, we speculate that, in frog tissue, D‐AspO plays an essential role in modulating the D‐Asp concentration. In addition, exaggerated D‐Asp concentrations activated SOD1 as cytoprotective mechanism in the kidney, whereas, in the brain and in the heart, where the antioxidant action of SOD1 is limited, caspase 3 was activated.
Collapse of Homochirality of Amino Acids in Proteins from Various Tissues during Aging
Authors: Fujii Noriko , Kaji Yuichi , Fujii Norihiko , Nakamura Tooru , Motoie Ryota , Mori Yuhei , Kinouchi Tadatoshi 
Published: June 2010

AbstractPrior to the emergence of life, it is believed that only L‐amino acids were selected for formation of proteins, and that D‐amino acids were eliminated on the primitive Earth. Whilst homochirality is essential for life, recently the occurrence of proteins containing D‐β‐aspartyl (Asp) residues from various tissues of elderly subjects has been reported. Here, we discuss the presence of D‐β‐Asp‐containing proteins in the lens, ciliary body, drusen, and sclera of the eye, skin, cardiac muscle, blood vessels of the lung, chief cells of the stomach, longitudinal and circular muscles of the stomach, and small and large intestines. Since the D‐β‐Asp residue occurs through a succinimide intermediate, this isomer may potentially be generated in proteins more easily than initially thought. UV Rays and oxidative stress can accelerate the formation of the D‐β‐Asp residue in proteins.
Thyroid Hormones and D‐Aspartic Acid, D‐Aspartate Oxidase, D‐Aspartate Racemase, H2O2, and ROS in Rats and Mice
Authors: Topo Enza , Fisher George , Sorricelli Andrea , Errico Francesco , Usiello Alessandro , D'Aniello Antimo 
Published: June 2010

AbstractTotal concentrations of thyroid hormones T3 and T4, and of their free forms, FT3 and FT4, D‐aspartic acid (D‐Asp), D‐aspartate oxidase (D‐AspO), D‐aspartate racemase, H2O2, and ROS (reactive oxygen species) were determined in rats and mice. T3 and T4 were 1 and 50 ng/ml, respectively, in serum, and 750 and 40000 ng/g, respectively, in thyroid. Concentrations of the free forms FT3 and FT4 were ca. 250 times lower than their respective total concentrations. The endogenous content of D‐Asp in thyroid gland was ca. 100 nmol/g tissue, whereas the activity of D‐AspO was ca. 80 units/mg thyroid, and that of D‐aspartate racemase was ca. 15 units/mg thyroid. H2O2 Concentration in rat and mouse thyroid gland was ca. 290 pmol/g thyroid, and the concentration of ROS was ca. 10 pmol/DCF/min/mg protein. H2O2 is essential for the iodination of the tyrosyl residues to produce mono‐ and diiodotyrosine that are the precursors for the synthesis of T3 and T4. Production of H2O2 in thyroid glands occurs by oxidation of endogenous D‐Asp by D‐AspO (D‐Asp+O2+H2O→α‐oxaloacetate+NH3+H2O2). D‐Aspartate racemase catalyzes the in vivo production of D‐Asp from L‐Asp. Thus, interaction of endogenous D‐Asp, D‐AspO, and D‐aspartate racemase in thyroid gland constitutes an additional biochemical pathway for the production of H2O2 and consequently for the synthesis of thyroid hormones.
ChemInform Abstract: Evidence for an Isostructural Phase Transition in the Metastable High‐Temperature Modification of TbPdAl.
Authors: Doenni A. , Kitazawa H. , Fischer P. , Fauth F. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: High‐Pressure Study of Titanium Carbide.
Authors: Dubrovinskaia N. A. , Dubrovinsky L. S. , Saxena S. K. , Ahuja R. , Johansson B. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Novel Dimeric Ni‐Substituted β‐Keggin Silicotungstate: Structure and Magnetic Properties of K12 [{β‐SiNi2W10O36(OH)2 (H2O)}2]×20H2O.
Authors: Kortz Ulrich , Jeannin Yves P. , Teze Andre , Herve Gilbert , Isber Samih 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and Structure of [{Mo(CO)4}2 (cis‐μ‐F2SbF4)3]x [Sb2F11]x: An Ionic Coordination Polymer.
Authors: Broechler Raimund , Freidank Daniel , Bodenbinder Matthias , Sham Iona H. T. , Willner Helge , Rettig Steven J. , Trotter James , Aubke Friedhelm 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: An EXAFS Study of AgPO3 and AgBaP3O9.
Authors: Belharouak I. , Fargin E. , Parent C. , Le Flem G. , Aouad H. , Mesnaoui M. , Maazaz M. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Room Temperature Sonoelectrochemical Synthesis of Molybdenum Sulfide Fullerene‐Like Nanoparticles.
Authors: Mastai Yitzhak , Homyonfer Moshe , Gedanken Aharon , Hodes Gary 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Photoinduced Intramolecular Arene‐Olefin meta‐Cycloaddition of 5‐Phenyl‐fluorinated‐pent‐1‐enes.
Authors: Guo Xiao‐Chuan , Chen Qing‐Yun 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Reduction of Aromatic Azo‐, Azoxy‐, and Nitrocompounds by Ultrasonically Activated Nickel.
Authors: Wang Xiaolun , Xu Minghua , Lian Hongzhen , Pan Yi , Shi Yaozeng 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Chemistry of Fluorinated Vinamidinium Salts: Novel Reactions of β‐Trifluoromethyl Vinamidinium Salt with Grignard Reagents.
Authors: Yamanaka Hiroki , Uegaki Tomonori , Ishihara Takashi , Kubota Toshio , Gupton John T. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: One‐Pot Deprotective Oxidation of O‐Allyl Ethers Using 70% tert‐Butyl Hydroperoxide and Catalytic CrO3.
Authors: Chandrasekhar S. , Mohanty Pradyumna K. , Ramachander T. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.