bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk of prostate cancer is not associated with levels of C‐reactive protein and other commonly used markers of inflammation
Authors: Van Hemelrijck Mieke , Jungner Ingmar , Walldius Göran , Garmo Hans , Binda Elisa , Hayday Adrian , Lambe Mats , Holmberg Lars , Hammar Niklas 
Published: November 2010

AbstractMost population‐based studies studied the association between inflammation and prostate cancer (PCa) by assessing C‐reactive protein (CRP). As these findings have shown inconsistent results, we aimed to also study different markers that have been commonly taken as indications of inflammation. A cohort based on four groups of men (n = 34,891), according to age at cohort entry (45, 55, 65 and 75 years), with measurements of glucose, triglycerides, total cholesterol, haptoglobin, albumin, hemoglobin and leukocytes were selected from the Apolipoprotein Mortality Risk database. A total of 17,937 men had measurements of non‐high‐sensitive CRP. Multivariate Cox proportional hazard models were used to analyze associations between inflammatory markers and PCa. A total of 49 of 12,063 men developed PCa in the age 45 group, whereas 207 of 9,940, 472 of 8,266 and 276 of 3,618 were diagnosed in the age 55, 65 and 75 groups, respectively. Mean follow‐up time was 7.5 years (SD: 3.9). No markers showed an association with PCa risk, nor was there a trend by quartiles or an indication for different PCa risks by strata of hypercholesterolemia, hyperglycemia and hypertriglyceridemia status. The studied markers were not found to be associated with PCa risk. These null findings might be due to methodological issues; however, it is unlikely that strong and long‐lasting associations between inflammation and PCa risk were missed as this was a large database with long follow‐up. This indicates need for international consensus on appropriate inflammatory markers in the context of cancer that may be practically applied in large studies.
Direct anti‐cancer effect of oncostatin M on chondrosarcoma
Authors: David Emmanuelle , Guihard Pierre , Brounais Bénédicte , Riet Anne , Charrier Céline , Battaglia Séverine , Gouin François , Ponsolle Stéphanie , Bot Ronan Le , Richards Carl D. , Heymann Dominique , Rédini Françoise , Blanchard Frédéric 
Published: November 2010

AbstractThe cytokine Oncostatin M (OSM) is cytostatic, pro‐apoptotic and induces differentiation of osteosarcoma cells into osteocytes, suggesting new adjuvant treatment for these bone‐forming sarcomas. However, OSM systemic over‐expression could lead to adverse side effects such as generalized inflammation, neoangiogenesis and osteolysis. We determine here the effect of OSM on chondrosarcoma, another primary bone sarcoma characterized by the production of cartilage matrix and altered bone remodelling. Chondrosarcomas are resistant to conventional chemotherapy and radiotherapy, and wide surgical excision remains the only available treatment. We found that OSM blocked the cell cycle in four of five chondrosarcoma cell lines, independently of p53 and presumably through the JAK3/STAT1 pathway. In two tested cell lines, OSM induced a hypertrophic chondrocyte differentiation, with an induced Cbfa1/SOX9 ratio and induced Coll10, matrix metalloproteinase 13 (MMP13) and RANKL expression. Adenoviral gene transfer of OSM (AdOSM) in the Swarm rat chondrosarcoma (SRC) model indicated that local intra‐tumoral OSM over‐expression reduces chondrosarcoma development not only with reduced tumor proliferation and enhanced apoptosis but also with enhanced RANKL expression, osteoclast formation and reduced bone volumes. Flu‐like symptoms were induced by the AdOSM, but there was no effect on tumor angiogenesis. Therefore, OSM could be considered as a new adjuvant anti‐cancer agent for chondrosarcomas. A local application of this cytokine is presumably needed to overcome the poor vascularization of these tumors and to limit the deleterious effect on other tissues. Its side effect on bone remodeling could be managed with anti‐resorption agents, thus offering potential new lines of therapeutic interventions.
Seromic profiling of colorectal cancer patients with novel glycopeptide microarray
Authors: Pedersen Johannes W. , Blixt Ola , Bennett Eric P. , Tarp Mads A. , Dar Imran , Mandel Ulla , Poulsen Steen S. , Pedersen Anders E. , Rasmussen Susanne , Jess Per , Clausen Henrik , Wandall Hans H. 
Published: November 2010

AbstractCancer‐associated autoantibodies hold promise as sensitive biomarkers for early detection of cancer. Aberrant post‐translational variants of proteins are likely to induce autoantibodies, and changes in O‐linked glycosylation represent one of the most important cancer‐associated post‐translational modifications (PTMs). Short aberrant O‐glycans on proteins may introduce novel glycopeptide epitopes that can elicit autoantibodies because of lack of tolerance. Technical barriers, however, have hampered detection of such glycopeptide‐specific autoantibodies. Here, we have constructed an expanded glycopeptide array displaying a comprehensive library of glycopeptides and glycoproteins derived from a panel of human mucins (MUC1, MUC2, MUC4, MUC5AC, MUC6 and MUC7) known to have altered glycosylation and expression in cancer. Seromic profiling of patients with colorectal cancer identified cancer‐associated autoantibodies to a set of aberrant glycopeptides derived from MUC1 and MUC4. The cumulative sensitivity of the array analysis was 79% with a specificity of 92%. The most prevalent of the identified autoantibody targets were validated as authentic cancer immunogens by showing expression of the epitopes in cancer using novel monoclonal antibodies. Our study provides evidence for the value of glycopeptides and other PTM‐peptide arrays in diagnostic measures.
Toxic effects of lead and nickel nitrate on rat liver chromatin components
Authors: Rabbani‐Chadegani III Azra , Fani Nesa , Abdossamadi Sayeh , Shahmir Nosrat 
Published: November 2010

AbstractThe biological activity of heavy metals is related to their physicochemical interaction with biological receptors. In the present study, the effect of low concentrations of nickel nitrate and lead nitrate (<0.3 mM) on rat liver soluble chromatin and histone proteins was examined. The results showed that addition of various concentrations of metals to chromatin solution preceded the chromatin into aggregation and precipitation in a dose‐dependant manner; however, the extent of absorbance changes at 260 and 400 nm was different between two metals. Gel electrophoresis of histone proteins and DNA of the supernatants obtained from the metal‐treated chromatin and the controls revealed higher affinity of lead nitrate to chromatin compared to nickel nitrate. Also, the binding affinity of lead nitrate to histone proteins free in solution was higher than nickel. On the basis of the results, it is concluded that lead reacts with chromatin components even at very low concentrations and induce chromatin aggregation through histone–DNA cross‐links. Whereas, nickel nitrate is less effective on chromatin at low concentrations, suggesting higher toxicity of lead nitrate on chromatin compared to nickel. © 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:127–134, 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20368
A comparative study of DNA binding and cell cycle phase perturbation by the dinuclear complex of Cd(II) with the condensation product of 2‐acetylpyridine and malonic acid dihydrazide N′,N′2‐bis[(1E)‐1‐(2‐pyridyl)ethylidene]propanedihydrazide
Authors: Vujčić Miroslava , Lazić Milan , Milenković Milica , Sladić Dušan , Radulović Siniša , Filipović Nenad , Anđelković Katarina 
Published: November 2010

AbstractOrganometallic Cd(II) compounds have recently attracted attention for their anticancer activity. The interaction of the dinuclear complex of Cd(II) with the condensation product of 2‐acetylpyridine and malonic acid dihydrazide, N′,N′2‐bis[(1E)‐1‐(2‐pyridyl)ethylidene]propanedihydrazide (Cd(II)H2L), with calf thymus DNA (CT‐DNA) was monitored by blue shift in UV–vis spectra of the complex. The binding constant of Cd(II)H2L complex with CT‐DNA was determined (KB = 1.8 × 104 M−1) and was indicative of minor groove binding. Agarose gel electrophoretic changes in mobility of supercoiled and circular forms of pBR322 and pUC18 plasmids in the presence of the complex suggest that conformational changes in the plasmids occur upon binding of the Cd(II)H2L complex. The Cd(II)H2L complex induced perturbation of the cell cycle phase distribution and an increase in the percentage of cells in the sub‐G1 phase of human cervical cancer HeLa cell line and murine melanoma B16 cell line. Immunoblotting analysis showed the overexpression of Bcl‐2 protein with the Cd(II)H2L complex. © 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:175–182 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20374
Ultrastructural comparison of the ocelli ofSinopanorpa tinctaandBittacus planus(Mecoptera)
Authors: Wei Yao , Hua Baozhen 
Published: November 2010

AbstractOn the contrary to compound eyes, dorsal ocelli are poorly investigated for their ultrastructure in many insect groups, including Mecoptera. The ocellar ultrastructure of the scorpionflySinopanorpa tincta(Navás) in Panorpidae and the hangingflyBittacus planus(Cheng) in Bittacidae was examined using transmission electron microscope. Both species possess three ocelli on the vertex of their heads and each ocellus has a corneal lens, some pigment cells at the outer rim of the lens, and fused rhabdoms. Beneath the corneal lens lies a single layer of corneagenous cells, which is very thick with the nuclei located proximally inS. tinctaand quite thin inB. planus. A portion of rhabdom is composed of rhabdomeres from two adjacent retinula cells inS. tincta, but from four retinula cells inB. planus. Each rhabdomere comprises parallel microvilli projecting laterally from the apposed retinula cells. In addition, abundant electron‐lucent granules are present at the middle part around each retinula inB. planus. Only pigment granules exist around the retinula proximally at the nucleate level inS. tincta. Microsc. Res. Tech., 2010. © 2010 Wiley‐Liss, Inc.
Observation particle morphology of colloidal system by conventional SEM with an improved specimen preparation technique
Authors: Xu Jing , Hou Zhaosheng , Yuan Xiaojiao , Guo Hong 
Published: November 2010

AbstractOn the basis of our previous report that polymer emulsion with different viscosity can be investigated by conventional scanning electron microscopy (SEM), we have developed an improved specimen preparation technique for obtaining particle morphology and size of colloidal silver, collagen, glutin, and polymer microspheres. In this study, we expect to provide a means for charactering the three‐dimensional surface microstructure of colloidal particles. Dilution of the samples with appropriate volatile solvent like ethanol is effective for SEM specimen preparation. At a proper ratio between sample and ethanol, the colloidal particles are dispersed uniformly in ethanol and then deposited evenly on the substrate. Different drying methods are studied to search a proper drying condition, in which the small molecule solvent is removed without destroying the natural particle morphology. And the effects of ethanol in the specimen preparation process are described by analyzing the physicochemical properties of ethanol. The specimen preparation technique is simple and can be achieved in common laboratory for charactering the particle morphology of colloidal system. Microsc. Res. Tech., 2011. © 2010 Wiley‐Liss, Inc.
A unique skeletal microstructure of the deep‐sea micrabaciid scleractinian corals
Authors: Janiszewska Katarzyna , Stolarski Jarosław , Benzerara Karim , Meibom Anders , Mazur Maciej , Kitahara Marcelo V. , Cairns Stephen D. 
Published: November 2010

AbstractMicrabaciids are solitary, exclusively azooxanthellate deep‐sea corals belonging to one of the deepest‐living (up to 5,000 m) scleractinian representatives. All modern micrabaciid taxa (genera: Letepsammia, Rhombopsammia, Stephanophyllia, Leptopenus) have a porous and often very fragile skeleton consisting of two main microstructural components known also from other scleractinians: rapid accretion deposits and thickening deposits. However, at the microstructural level, the skeletal organization of the micrabaciids is distinctly different from that of other scleractinians. Rapid accretion deposits consist of alternations of superimposed “microcrystalline” (micrometer‐sized aggregates of nodular nanodomains) and fibrous zones. In contrast to all shallow‐water and sympatric deep‐water corals so far described, the thickening deposits of micrabaciids are composed of irregular meshwork of short (1–2 μm) and extremely thin (ca. 100–300 nm) fibers organized into small, chip‐like bundles (ca. 1–2 μm thick). Longer axes of fiber bundles are usually subparallel to the skeletal surfaces and oriented variably in this plane. The unique microstructural organization of the micrabaciid skeleton is consistent with their monophyletic status based on macromorphological and molecular data, and points to a diversity of organic matrix‐mediated biomineralization strategies in Scleractinia. J. Morphol.,2011. © 2010 Wiley‐Liss, Inc.
Area, length and mineralization content of new bone at bone–tendon junction predict its repair quality
Authors: Lu Hongbin , Hu Jiangzhong , Qin Ling , Chan Kai Ming , Li Gang , Li Kanghua 
Published: November 2010

AbstractWe investigated the hypothesis that if the area, length, and mineralization of newly formed bone could be used to predict the healing quality of patella–patellar tendon (PPT) junction after partial patellectomy. Twenty‐four rabbits underwent partial patellectomy and their PPT complexes of the operated limbs were harvested at weeks 6, 12, and 18 postoperatively. The area, length, and mineralization of newly formed bone at PPT junction healing interface was evaluated radiographically and peripheral quantitative computational tomographically. The healing quality of PPT complexes in terms of its tensile property was determined by biomechanical testing. The results showed that the area, length, and mineral content of newly formed bone, and its tensile strength increased significantly with follow‐up time. The area of newly formed bone was strongly correlated with the failure load, ultimate strength and energy at failure (r = 0.75, 0.76, and 0.70, respectively, p < 0.01 for all). The length of newly formed bone was also found to be correlated with failure load, ultimate strength and energy at failure (r = 0.61, 0.54, 0.67, respectively, p < 0.01 for all). In addition, the bone mineral content of newly formed bone but not its bone mineral density was moderately correlated with failure load, ultimate strength and energy at failure (r = 0.44, 0.51, 0.42, respectively, p < 0.05 for all). In conclusion, the area, length, and mineralization of newly formed bone at the PPT junction after partial patellectomy may serve as useful noninvasive indices in assessing the quality of the bone–tendon junction repair. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:672–677, 2011
Room temperature ionic liquid‐mediated molecularly imprinted polymer monolith for the selective recognition of quinolones in pork samples
Authors: Sun Xiangli , He Jia , Cai Guorui , Lin Anqing , Zheng Wenjie , Liu Xuan , Chen Langxing , He Xiwen , Zhang Yukui 
Published: November 2010

AbstractA novel molecularly imprinted polymer monolith was prepared by the room temperature ionic liquid‐mediated in situ molecular imprinting technique, using norfloxacin (NOR) as the template, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross‐linker. The optimal synthesis conditions and recognition properties of NOR‐imprinted monolithic column were investigated. The results indicated that the imprinted monoliths exhibited good ability of selective recognition against the template and its structural analog. Using the fabricated material as solid‐phase extraction sorbent, a sample pre‐treatment procedure of molecularly imprinted solid‐phase extraction coupling with HPLC was developed for determination of trace quinolone residues in animal tissues samples. The recoveries ranging from 78.16 to 93.50% for eight quinolones antibiotics such as marbofloxacin, NOR, ciprofloxacin, danofloxacin, difloxacin, oxolinic acid, flumequine and enrofloxacin were obtained.
Determination of amoxapine and nortriptyline in blood plasma and serum by salt‐assisted liquid–liquid microextraction and high‐performance liquid chromatography
Authors: Gupta Manju , Jain Archana , Verma Krishna K. 
Published: November 2010

AbstractA new, selective and sensitive method has been developed for the determination of tricyclic antidepressant drugs, amoxapine and nortriptyline, in human blood plasma and serum, involving their reaction with allyl isothiocyanate and extraction of thiourea derivatives with water‐miscible organic solvent acetonitrile. The phase separation was effected by addition of ammonium sulphate, a process called salt‐assisted liquid–liquid microextraction. The extract was analyzed by HPLC with UV detection at 254 nm. The method has been optimized for derivatization reaction time and temperature, solvent for extraction, and salt for solvent phase separation. Under the optimal conditions, a linear calibration graph was obtained between the amount of drug and the peak area of thiourea derivatives in the range of 0.002–20 mg/L drugs. The correlation coefficient and limit of detection values for amoxapine and nortriptyline in serum/plasma samples were in the range of 0.9953–0.9999 and 0.46–0.58 μg/L, respectively. The recovery in spiking experiments ranged, respectively, 75–88% (RSD 3.4–7.2%) and 79–97% (RSD 3.7–7.9%) for the two drugs.
A weak cation‐exchange monolith as stationary phase for the separation of peptide diastereomers by CEC
Authors: Ludewig Ronny , Nietzsche Sandor , Scriba Gerhard K. E. 
Published: November 2010

AbstractA CEC weak cation‐exchange monolith has been prepared by in situ polymerization of acrylamide, methylenebisacrylamide and 4‐acrylamidobutyric acid in a decanol–dimethylsulfoxide mixture as porogen. The columns were evaluated by SEM and characterized with regard to the separation of diastereomers and α/β‐isomers of aspartyl peptides. Column preparation was reproducible as evidenced by comparison of the analyte retention times of several columns prepared simultaneously. Analyte separation was achieved using mobile phases consisting of acidic phosphate buffer and ACN. Under these conditions the peptides migrated due to their electrophoretic mobility but the EOF also contributed as driving force as a function of the pH of the mobile phase due to increasing dissociation of the carboxyl groups of the polymer. Raising the pH of the mobile phase also resulted in deprotonation of the peptides reducing analyte mobility. Due to these mechanisms each pair of diastereomeric peptides displayed the highest resolution at a different pH of the buffer component of the mobile phase. Comparing the weak‐cation exchange monolith to an RP monolith and a strong cation‐exchange monolith different elution order of some peptide diastereomers was observed, clearly illustrating that interactions with the stationary phase contribute to the CEC separations.
Gene expression in the oropharynx of children exposed to secondhand smoke
Authors: Ostrower Samuel T. , Fischer Tova C. , Smith Richard V. , Belbin Thomas J. , Bent John P. , Parikh Sanjay R. 
Published: November 2010

AbstractObjectives/Hypothesis:To compare gene expression in oropharyngeal mucosa of children with (ex+) and without (ex−) secondhand smoke exposure.Study Design:Prospective case‐control.Methods:Forty‐one age‐ and gender‐matched children (2–6 years old) undergoing tonsillectomy for sleep disordered breathing at a tertiary care children's hospital were assessed for secondhand smoke exposure. Parental response to a validated questionnaire relating to secondhand smoke exposure governed inclusion. Sixteen samples were selected for microarray analysis (7 ex+, 9 ex−). Following tonsillectomy, ex vivo brushing of the mucosa isolated total RNA. Genome‐wide expression profiles were generated by comparing sample RNA to a reference of all samples, assessing 27,323 cDNA clones. Microarray clones were ranked according to their ability to distinguish between the two groups using a Student t test.Results:A total of 318 cDNA clones distinguished the two groups (P < .01); 180 genes were overexpressed and 138 underexpressed in ex+ samples relative to the ex− group. Independent analysis of these two groups sorted genes into disease processes and molecular functional groups, including cancer (34 genes in the overexpressed group, 29 underexpressed, P < .05), cell cycle (14 and 10), and cell growth and proliferation (7 and 11). Two of the upregulated genes, LCN2 and IQGAP1, have been previously linked to inflammation in smokers and response/repair to cellular injury in bronchial epithelium.Conclusions:Findings in this pilot study support the hypothesis that secondhand smoke exposure seems to induce gene expression changes in the oropharyngeal mucosa of exposed children, which may have significant implications for current and future disease processes. Laryngoscope, 2010
Cochlear implantation in patients with autoimmune inner ear disease including cogan syndrome: A comparison with age‐ and sex‐matched controls
Authors: Wang Jenny R. , Yuen Heng W. , Shipp David B. , Stewart Suzanne , Lin Vincent Y. W. , Chen Joseph M. , Nedzelski Julian M. 
Published: November 2010

AbstractObjectives/Hypothesis:Evaluate the characteristics and outcomes of patients with autoimmune inner ear disease (AIED) who have undergone cochlear implantation (CI) and compare post‐CI performance in AIED to matched controls.Study Design:Retrospective case control study.Methods:Study cohort was comprised of 25 adult implantees (AIED [n = 18], Cogan syndrome [n = 7]). The AIED group was defined by rapidly progressive bilateral sensorineural hearing loss leading to unusable hearing within weeks to months. Patients with Cogan syndrome, the archetypal inner ear autoimmune disease, were also examined and used for within‐cohort comparison. Clinical and operative records were reviewed. Post‐CI performance was assessed using open‐set sentence tests. Age‐ and sex‐matched individuals deafened by other postlingual causes were used as controls.Results:Of 25 patients, 24 had uneventful, full electrode insertions. One AIED patient had partial insertion due to cochlear ossification and did not achieve open‐set speech perception post‐CI. Mean open‐set sentence scores for study patients with uneventful insertions were 92.8%, 97.3%, and 96.4% at 6 months, 1 year, and ≥2 years, respectively. Compared to matched controls, patients deafened by autoimmune causes had significantly higher post‐CI performance at all postoperative test intervals (P < .05). There was no significant difference in postimplantation performance between Cogan syndrome and AIED patients.Conclusions:To our knowledge this was the largest study of cochlear implantation in AIED and Cogan syndrome patients. In our experience, both groups generally attained high levels of post‐CI speech perception and performed above average. Cochlear ossification affecting implantation in Cogan syndrome patients was not observed in our series, contrary to some reports. Laryngoscope, 2010