bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of maternal age on the developmental competence and progression of nuclear maturation in bovine oocytes
Authors: Yamamoto T. , Iwata H. , Goto H. , Shiratuki S. , Tanaka H. , Monji Y. , Kuwayama T. 
Published: April 2010

AbstractProgression of meiotic division in oocytes and early embryonic development are affected by oocytes quality. In most mammals, oocyte quality declines with increase in maternal age. The main aim of the present study is to investigate the effect of maternal age on developmental competence, progression of meiotic division, and associated kinetics of maturation promoting factor (MPF) activity in bovine oocytes. Oocytes were collected from the ovaries of young and old cows (here after referred to as young cow oocytes and old cow oocytes, respectively). When old cow oocytes were matured and fertilized in vitro, the rate of abnormal fertilization was greater than that in young cow oocytes. Moreover, progression of nuclear maturation and activation of MPF during oocyte maturation (or inactivation of MPF and formation of pronucleus after insemination) were faster in old cow oocytes than in young cow oocytes. Relative expression of cyclin B, cyclin‐dependent kinase 1 and MAD2 transcripts in either immature or mature oocytes did not differ between the two groups. When cumulus cells (CC) were removed and denuded oocytes were cultured, there was no difference in the progression of nuclear maturation between the two age groups. Moreover gap junctions between oocytes and CC disappeared more rapidly during maturation of old cow oocytes than of young cow oocytes. These results suggest that the fertilization ability of old cow oocytes is low and that premature progression of meiotic division in these oocytes is partly due to impaired oocyte–CC gap junctions communication. Mol. Reprod. Dev. 77: 595–604, 2010. © 2010 Wiley‐Liss, Inc.
Vorinostat in der Behandlung kutaner T‐Zell‐Lymphome. Therapie mit Histon‐Desacetylase‐Inhibitoren
Authors: Zirlik Katja , Nashan Dorothée , Veelken Hendrik 
Published: April 2010

AbstractDerzeit werden verschiedene epigenetische Ansätze in der Krebstherapie untersucht. Im Fokus dieser Übersicht steht die Inhibition von Histon‐Desacetylasen, welche Genregulation und kontrollierten Zelltod (Apoptose) beeinflussen. Ein Vertreter dieser neuen Substanzklasse ist Vorinostat. In den USA ist die Substanz bereits für die Drittlinientherapie der kutanen T‐Zell‐Lymphome zugelassen.
Die Entzifferung der fünften DNA‐Base. Das Methylom des Menschen
Authors: Winckler Thomas , Zündorf Ilse , Dingermann Theo 
Published: April 2010

Abstract5‐Methylcytosin ist die “fünfte Base” in der DNA humaner Zellen. Die Methylierung bestimmter Cytosine im Genom unserer Zellen ändert nicht die Kodierung der in der DNA gespeicherten Information, spielt aber eine entscheidende Rolle bei der epigenetischen Transkriptionskontrolle. Fehlregulationen der epigenetischen Modulation der DNA können zu schweren Krankheiten beitragen. In diesem Artikel fassen wir zusammen, wie heute mit modernen Methoden der DNA‐Sequenzierung genomweite Unterschiede in der DNA‐Methylierung funktionell untersucht werden.
Supportive Therapie bei Myelodysplastischem Syndrom (MDS). Unverzichtbare Säule im multimodalen Behandlungskonzept
Authors: Göbel Alexandra , Lubrich Beate 
Published: April 2010

AbstractDas Myelodysplastische Syndrom (MDS) umfasst eine heterogene Gruppe von Stammzellerkrankungen. Onkogene Transformation von hämatopoetischen Stammzellen führt zur Störung von Proliferation, Differenzierung und Apoptose. Die Hämatopoese ist stark beeinträchtigt und es besteht ein hohes Risiko für die Entwicklung einer akuten myeloischen Leukämie (AML).
Mit Antisense gegen Krebs?
Authors: Edris E. , Möller A.‐M. , Oberhauser E. M. , Zündorf, I. , Dingermann T. 
Published: April 2010

AbstractMittlerweile sind bereits ein paar Wirkstoffe zugelassen, die die Versorgung eines soliden Tumors mit Sauerstoff unterbinden, vor allem über die Unterdrückung des Wachstums von Blutgefäßen in das Tumorgewebe. Eine ganz andere Zielstruktur wird jetzt mit EZN‐2968 attackiert: die mRNA des Hypoxie‐induzierbaren Faktors 1α.
Neuer Ansatz zur Bestimmung der pharmakodynamischen Effekte von Histon‐Desacetylase‐Hemmstoffen
Authors: Wagner Julia M. , Jung Manfred 
Published: April 2010

AbstractHemmstoffe der Histon‐Desacetylasen (HDACi) traten in den letzten Jahren vermehrt in die klinischen Phasen der Arzneistoffprüfung ein. Erste Inhibitoren (Vorinostat, Zolinza™; Romidepsin, Istodax™) sind bereits für die Behandlung des kutanen T‐Zell‐Lymphoms (CTCL) zugelassen.
Rauschfreie Röntgenbilder mit Medipix. Bildgebende Pixeldetektoren
Authors: Anton Gisela , Michel Thilo 
Published: April 2010

AbstractDie photonenzählenden, bildgebenden Pixeldetektoren entstammen einer Entwicklung aus der Teilchenphysik. Sie bieten als bildgebende Verfahren mannigfaltige Anwendungsmöglichkeiten im Bereich der Grundlagenforschung, Medizin und Materialforschung. Ein gutes Beispiel sind die Generationen des Medipix‐Röntgendetektors, die in einer großen Kollaboration aus dem CERN heraus entstanden sind. Ein besonderer Vorteil ist ihre Fähigkeit, auch schwache Röntgensignale in rauschfreie Bilder umzusetzen. Die Technik ermöglicht eine hohe örtliche, zeitliche und energetische Auflösung der Messsignale. Ein Hybriddetektor auf Basis der Medipix‐Technologie kann auch einzelne Photonen des sichtbaren Spektrums hochempfindlich messen.
Heiße Action dank cooler Physik. Eishockey‐Weltmeisterschaft 2010
Authors: Mathelitsch Leopold , Thaller Sigrid 
Published: April 2010

AbstractEishockey ist die schnellste Mannschaftssportart. Eis ist sehr gleitfähig, weil seine Oberfläche ein quasiflüssiger Wassermolekülfilm überzieht, der leicht verschiebbar ist. Für Geschwindigkeit sorgen zudem Schlagschüsse, bei denen der gebogene Schläger dem Puck wie eine Feder zusätzliche Energie mitgibt. Die Schutzkleidung des Tormanns bremst den Puck beim Aufprall. Sie erlaubt ihm aber auch, das Tor besser zu verdecken.
Farbige Moiré‐Muster als Naturphänomen. Spielwiese
Authors: Schlichting Hans‐Joachim , Suhr Wilfried 
Published: April 2010

AbstractKonzentrische Ringwellen auf der Oberfläche eines Schwimmbeckens können ein überraschendes Moiré‐Muster erzeugen. Es ähnelt der Überlagerung zweier Kreissysteme, die aus Fresnelschen Zonenplatten bestehen. Da die ringförmigen Wellen wie Zylinderlinsen wirken, ruft die Dispersion besonders an den Kreuzungspunkten zwischen den Ringen starke Farbränder hervor. Aus der Distanz führen diese zu dem in Spektralfarben erscheinenden Ringsystem.
Aufgeblasene Pommes
Authors: Vilgis Thomas 
Published: April 2010

AbstractDie Verwandlung von Kartoffelscheiben in aufgeblasene knusprige Kissen ist die Krönung der Kartoffelküche. “Pommes Soufflées” gelten stets als großes Beiwerk in der Haute Cuisine. Deren Herstellung ist auch ein Paradebeispiel angewandter Kochphysik. Zweimaliges “Frittieren” in unterschiedlich heißen Ölbädern ist die Krux dieses Experiments.
The secreted proteome profile of developing Dictyostelium discoideum cells
Authors: Bakthavatsalam Deenadayalan , Gomer Richard H. 
Published: April 2010

AbstractDictyostelium discoideum is a unicellular eukaryote that, when starved, aggregates to form multicellular structures. In this report, we identified the proteins secreted by developing Dictyostelium cells using MS‐based proteomics. A total of 349 different secreted proteins were identified, indicating that at least 2.6% of the 13 600 predicted proteins in the Dictyostelium genome are secreted. Gene ontology analysis suggests that many of the secreted proteins are involved in protein and carbohydrate metabolism, and proteolysis.
Mitochondrial oxidative phosphorylation system is recruited to detergent‐resistant lipid rafts during myogenesis
Authors: Kim Bong‐Woo , Lee Joong‐Won , Choo Hyo‐Jung , Lee Chang Seok , Jung Soon‐Young , Yi Jae‐Sung , Ham Young‐Mi , Lee Joo‐Hyung , Hong Jin , Kang Min‐Ju , Chi Sung‐Gil , Hyung Seok‐Won , Lee Sang‐Won , Kim Hwan Myung , Cho Bong Rae , Min Do‐Sik , Yoon Gyesoon , Ko Young‐Gyu 
Published: April 2010

AbstractSince detergent‐resistant lipid rafts play important roles in the signal transduction for myogenesis, their comprehensive proteomic analysis could provide new insights to understand their function in myotubes. Here, the detergent‐resistant lipid rafts were isolated from C2C12 myotubes and analyzed by capillary RPLC/MS/MS. Among the 327 proteins (or protein groups) identified, 28% were categorized to the plasma membrane or raft proteins, 29% to mitochondria, 20% to microsomal proteins, 10% to other proteins, and 13% to unknown proteins. The localization of oxidative phosphorylation (OXPHOS) complexes in the sarcolemma lipid rafts was further confirmed from C2C12 myotubes by cellular fractionation, surface‐biotin labeling, immunofluorescence, and lipid raft fractionation. After adding exogenous cytochromec, the sarcolemma isolated from myotubes had an ability to consume oxygen in the presence of NADH or succinate. The generation of NADH‐dependent extracellular superoxide was increased by inhibiting or downregulating OXPHOS I, III, and IV in myotubes, indicating that OXPHOS proteins are major sources for extracellular ROS in skeletal muscle. With all these data, we can conclude that OXPHOS proteins are associated with the sarcolemma lipid rafts during C2C12 myogenesis to generate extracellular ROS.
Scanning X‐ray fluorescence microspectroscopy of metallic impurities in solar‐grade silicon
Authors: Macdonald Daniel , Rougieux Fiacre , Mansoulie Yves , Tan Jason , Paterson David , Howard Daryl L. , de Jonge Martin D. , Ryan Chris G. 
Published: April 2010

AbstractA rapid scanning synchrotron‐based X‐ray fluorescence microprobe technique is applied to relatively impure crystalline silicon feedstock for solar cells. The results reveal the distributions of metallic impurities in the material over regions several millimetres in size, allowing scans across several grains. Relatively high concentrations of Fe, Cu and Zn were observed, with traces of Mn and Ni. The metals were mostly present as discrete particles up to 60 µm in size, while Cu was more uniformly distributed. More than 50% of the detected Fe was present as large particles at the grain boundaries, probably due to diffusion and precipitation during cooling. In contrast, less than 5% of the Cu resided in such large particles. The particles contained multiple metallic elements, with strongly varying proportions of their metal constituents.
Perinatal and early surgical outcome for the fetus with hypoplastic left heart syndrome: a 5‐year single institutional experience
Authors: Rychik J. , Szwast A. , Natarajan S. , Quartermain M. , Donaghue D. D. , Combs J. , Gaynor J. W. , Gruber P. J. , Spray T. L. , Bebbington M. , Johnson M. P. 
Published: April 2010

AbstractObjectivesTo review our experience with the prenatal diagnosis of hypoplastic left heart syndrome (HLHS). Our goal was to establish the benchmark for perinatal and early surgical outcome in the current era, from a center with an aggressive surgical approach and a cohort with a high level of intention‐to‐treat.MethodsOutcome was assessed in fetuses with HLHS following stratification into high‐risk and standard‐risk categories. High risk was defined as the presence of any of the following: extracardiac, genetic or chromosomal anomalies; prematurity of < 34 weeks' gestation; additional cardiac findings such as intact or highly restrictive atrial septum, severe degree of tricuspid regurgitation or severe ventricular dysfunction. Standard risk was defined as absence of these risk factors.ResultsOf 240 fetuses evaluated over 5 years, 162 (67.5%) were in the standard‐risk group and 78 (32.5%) were in the high‐risk group. Of the 240 sets of parents, 38 (15.8%) chose termination or non‐intervention at birth at initial prenatal counseling and 185 of the neonates (77.1%) underwent first‐stage Norwood surgery with 155 surviving and 30 deaths, giving an overall Norwood operative survival of 83.8%. Breakdown by risk class reveals a significant Norwood operative survival advantage for the standard‐risk group (92.8%) over the high‐risk group (56.5%) (P < 0.001).ConclusionsFollowing prenatal diagnosis of HLHS, families should be strongly encouraged to undertake comprehensive prenatal evaluation in order to obtain an accurate prognosis. One‐third have additional risk factors that limit survival outcome, however two‐thirds do not and have an excellent chance of early survival. Copyright © 2010 ISUOG. Published by John Wiley & Sons, Ltd.
Crystal Structure and Formation of TlPd3and its new Hydride TlPd3H
Authors: Kurtzemann Nadine , Kohlmann Holger 
Published: April 2010

AbstractTlPd3was synthesised from the elements in evacuated silica tubes at 600 °C. Alternatively, TlPd3was yielded by reduction of TlPd3O4in N2gas atmosphere. Reduction of the oxide in H2gas atmosphere resulted in the formation of the new hydride TlPd3H. The structure of tetragonal TlPd3(ZrAl3type, space groupI4/mmm,a= 410.659(9) pm,c= 1530.28(4) pm) was reinvestigated by X‐ray and also by neutron powder diffraction as well as the structure of its previously unknown hydride TlPd3H (cubicanti‐perovskite type structure, space groupPm\bar{3}m,a= 406.313(1) pm). In situ DSC measurements of TlPd3in hydrogen gas atmosphere showed a broad exothermic signal over a wide temperature range with two maxima at 280 °C and at 370 °C, which resulted in the product TlPd3H. A dependency of lattice parameters of the intermetallic phase on reaction conditions is observed and discussed. Results of hydrogenation experiments at room temperature with gas pressures up to 280 bar hydrogen and at elevated temperatures with very low hydrogen gas pressures (1–2 bar) as well as results of dehydrogenation of the hydrides under vacuum will be discussed.
Exploration Of Factors Related To Hara-Kiri As A Method Of Suicide And Suicidal Behavior
Authors: Takai M. , Yamamoto K. , Iwamitsu Y. , Miyaji S. , Yamamoto H. , Tatematsu S. , Yukawa M. , Ide A. , Kamijo Y. , Soma K. , Miyaoka H. 
Published: April 2010

AbstractObjectiveThe objective of this study was to explore factors associated with hara-kiri as a method of suicide and suicidal behavior in contemporary Japan.MethodsA retrospective study was conducted on medical records of 421 patients (174 male; 247 female) who were considered suicidal and treated at the Kitasato University Hospital Emergency Medical Center in Japan between January 2006 and March 2008. We compared hara-kiri and all other methods regarding sociodemographics and clinical features of all suicidal patients.ResultsInstances of hara-kiri suicide attempt had the highest proportion of males (63%) among all suicide and suicidal behavior. One-way analysis of variance revealed significant differences between hara-kiri and other suicide attempt methods in the age of the suicidal patients. Result of multiple logistic regression analysis indicated that those who attempted hara-kiri suicide were likely to be male, be diagnosed with schizophrenia, survive, and be married.ConclusionOur findings indicate that hara-kiri as a method of suicide and suicidal behavior remain prevalent in Japan, and the study findings also suggest that both clinical and cultural factors might play a role in hara-kiri as a method of suicide and suicidal behavior.
The Response of Corporate Financing and Investment to Changes in the Supply of Credit
Authors: Lemmon Michael , Roberts Michael R. 
Published: April 2010

AbstractWe examine how shocks to the supply of credit impact corporate financing and investment using the collapse of Drexel Burnham Lambert, Inc.; the passage of the Financial Institutions Reform, Recovery, and Enforcement Act of 1989; and regulatory changes in the insurance industry as an exogenous contraction in the supply of below-investment-grade credit after 1989. A difference-in-differences empirical strategy reveals that substitution to bank debt and alternative sources of capital (e.g., equity, cash balances, and trade credit) was limited, leading to an almost one-for-one decline in net investment with the decline in net debt issuances. Despite this sharp change in behavior, corporate leverage ratios remained relatively stable, a result of the contemporaneous decline in debt issuances and investment. Overall, our findings highlight how even large firms with access to public credit markets are susceptible to fluctuations in the supply of capital.
Testing all six person-oriented principles in dynamic factor analysis
Authors: Molenaar Peter C. M. 
Published: April 2010

AbstractAll six person-oriented principles identified by Sterba and Bauer's Keynote Article can be tested by means of dynamic factor analysis in its current form. In particular, it is shown how complex interactions and interindividual differences/intraindividual change can be tested in this way. In addition, the necessity to use single-subject methods in the analysis of developmental processes is emphasized, and attention is drawn to the possibility to optimally treat developmental psychopathology by means of new computational techniques that can be integrated with dynamic factor analysis.
Developing the person-oriented approach: Theory and methods of analysis
Authors: von Eye Alexander 
Published: April 2010

AbstractThe development of paradigms, or perspectives of research takes place at the level of theory, in the domain of methodology, and in the context of existing paradigms and perspectives. The development of the person-oriented approach has made considerable progress at the level of theory. In addition, the approach has found a large number of applications. Sterba and Bauer's Keynote Article has closed a gap by discussing methodological implications of the person-oriented approach. In particular, the authors have discussed whether and, if yes, how the tenets of the person-oriented approach can be tested using tools of applied statistics popular in current empirical psychological research. Continuing this discussion, this article focuses on recent developments in all three areas. First, the importance and the implications of the concept of dimensional identity are discussed. It is argued that dimensional identity needs to be established across time and individuals for comparisons to be valid, both in person-oriented and in variable-oriented research. Second, methods not covered in Sterba and Bauer's Keynote are discussed and their application is exemplified. One focus of this discussion is on configural frequency analysis, which allows researchers to make statements about particular cells or groups of cells in cross-classifications of categorical variables. Third, person-oriented research is compared to differential psychology. It is argued that the concept of dimensional identity represents the next step in the development of a psychological subdiscipline that allows one to consider that individuals differ and develop in unique ways. These differences not only manifest in means but in any parameter, including covariance structures, and they can also manifest in the differential meaningfulness of variables for the description of individuals.
Is adolescence-onset antisocial behavior developmentally normative?
Authors: Roisman Glenn I. , Monahan Kathryn C. , Campbell Susan B. , Steinberg Laurence , Cauffman Elizabeth ,   The National Institute of Child Health and Human Development Early Child Care Research Network
Published: April 2010

AbstractLargely because of the influence of Moffitt's useful distinction between adolescence-limited and life-course persistent antisocial behavior, it has become increasingly common to view problem behavior that makes its first appearance in adolescence as developmentally normative. This study prospectively examined the lives of individuals in the NICHD Study of Early Child Care and Youth Development whose patterns of antisocial behavior varied with respect to age of onset and stability from kindergarten through age 15. Consistent with past research, early-onset, persistently deviant youth experienced more contextual adversity and evinced higher levels of intraindividual disadvantages than their peers from infancy through midadolescence. However, relative to youth who never showed significantly elevated antisocial behavior through age 15, children who showed antisocial behavior primarily in adolescence also were more disadvantaged from infancy forward, as were youth who only demonstrated significant externalizing problems in childhood. Findings generally replicated across sex and did not vary as a function of whether antisocial behavior groups were defined using T-scores normed within sex or identified using an empirically driven grouping method applied to raw data.
Time-dependent changes in positively biased self-perceptions of children with attention-deficit/hyperactivity disorder: A developmental psychopathology perspective
Authors: Hoza Betsy , Murray-Close Dianna , Arnold L. Eugene , Hinshaw Stephen P. , Hechtman Lily ,   The MTA Cooperative Group
Published: April 2010

AbstractThis study examined changes in the degree of positive bias in self-perceptions of previously diagnosed 8- to 13-year-old children with attention-deficit/hyperactivity disorder (ADHD;n= 513) and comparison peers (n= 284) over a 6-year period. The dynamic association between biased self-perceptions and dimensional indices of depressive symptoms and aggression also were considered. Across the 6-year time span, comparison children exhibited less bias than children with ADHD, although a normative bolstering of social self-views during early adolescence was observed. Decreases in positive biases regarding social and behavioral competence were associated with increases in depressive symptoms over time, whereas increases in levels of positively biased self-perceptions in the behavioral (but not social) domain were predictive of greater aggression over time. ADHD status moderated the dynamic association between biases and adjustment. Finally, evidence indicated that there was a bidirectional relationship between biases and aggression, whereas depressive symptoms appeared to inversely predict later bias.
Sulfite Reductase Defines a Newly Discovered Bottleneck for Assimilatory Sulfate Reduction and Is Essential for Growth and Development inArabidopsis thaliana
Authors: Khan Muhammad Sayyar , Haas Florian Heinrich , Allboje Samami Arman , Moghaddas Gholami Amin , Bauer Andrea , Fellenberg Kurt , Reichelt Michael , HÄnsch Robert , Mendel Ralf R. , Meyer Andreas J. , Wirtz Markus , Hell Rüdiger 
Published: April 2010

AbstractThe role of sulfite reductase (SiR) in assimilatory reduction of inorganic sulfate to sulfide has long been regarded as insignificant for control of flux in this pathway. Two independent Arabidopsis thaliana T-DNA insertion lines (sir1-1 and sir1-2), each with an insertion in the promoter region of SiR, were isolated. sir1-2 seedlings had 14% SiR transcript levels compared with the wild type and were early seedling lethal. sir1-1 seedlings had 44% SiR transcript levels and were viable but strongly retarded in growth. In mature leaves of sir1-1 plants, the levels of SiR transcript, protein, and enzymatic activity ranged between 17 and 28% compared with the wild type. The 28-fold decrease of incorporation of 35S label into Cys, glutathione, and protein in sir1-1 showed that the decreased activity of SiR generated a severe bottleneck in the assimilatory sulfate reduction pathway. Root sulfate uptake was strongly enhanced, and steady state levels of most of the sulfur-related metabolites, as well as the expression of many primary metabolism genes, were changed in leaves of sir1-1. Hexose and starch contents were decreased, while free amino acids increased. Inorganic carbon, nitrogen, and sulfur composition was also severely altered, demonstrating strong perturbations in metabolism that differed markedly from known sulfate deficiency responses. The results support that SiR is the only gene with this function in the Arabidopsis genome, that optimal activity of SiR is essential for normal growth, and that its downregulation causes severe adaptive reactions of primary and secondary metabolism.