bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of a guided internet self‐treatment programme for bulimia nervosa in several European countries
Authors: Carrard I. , Fernandez‐Aranda F. , Lam T. , Nevonen L. , Liwowsky I. , Volkart A. C. , Rouget P. , Golay A. , Van der Linden M. , Norring C. 
Published: September 2010

AbstractObjectiveThe purposes of this study were to evaluate the use of an online guided self‐treatment programme for bulimia nervosa (BN) and to determine predictors of outcome. Data were collected in four European countries where the programme was simultaneously used.MethodOne hundred and twenty‐seven BN or subthreshold BN female patients (mean age of 24.7 years) participated in a 4‐month intervention using a CBT based online‐guided self‐help programme. Contact during the treatment period included weekly e‐mails with a coach.AssessmentMeasures included the Eating Disorders Inventory‐2 (EDI‐2) and the Symptom Check List‐Revised (SCL‐90R).ResultsSeverity of eating disorders symptoms and general psychopathology improved significantly. Twenty‐three per cent of patients were symptom free at the end of treatment. The dropout rate was 25.2%. A better score of general psychological health was a predictor of a better outcome.ConclusionsThis study encourages further developments and research on innovative therapy approaches, particularly for those disorders such as BN, with difficult therapy and unclear prognosis. Copyright © 2010 John Wiley & Sons, Ltd and Eating Disorders Association.
A channel head stability criterion for first‐order catchments
Authors: Sólyom Péter B. 
Published: September 2010

AbstractThe present analysis derives a stability criterion for long‐term equilibrium channel heads. The concept of finite perturbation analysis is presented, during which the surface is subjected to perturbations of a finite amplitude and resulting changes in flow path structure and slope are computed. Based on these quantities the analysis predicts whether the perturbed location is going to erode, be filled in or remain steady. The channel head is defined geometrically as the focus point of converging flow lines at the bottom of hollows. It is demonstrated that stability at the channel head grows out of the competition between the rate of flow path convergence and the degree of profile concavity. Analytical functions are derived to compute channel head‐contributing area and ‐slope, flow path convergence and profile concavity as a function of perturbation depth, distance from the crest and the initial slope. In a numerical model these quantities point to the long‐term equilibrium channel head position, which is shown to depend also on the width to length ratio of hollows. It is also demonstrated that the equilibrium channel head position is sensitive to the base‐level lowering/non‐dimensional slope length ratio and to the slope of the initial topography. Morphometrical measurements both in the field and on simulated topographies were used to test the theoretical predictions.
Development of Nafion/Tin Oxide Composite MEA for DMFC Applications
Authors: Chen F. , Mecheri B. , D'Epifanio A. , Traversa E. , Licoccia S. 
Published: September 2010

AbstractNafion composite membranes containing either hydrated tin oxide (SnO2 · nH2O) or sulphated tin oxide (S–SnO2) at 5 and 10 wt.‐% were prepared and characterised. The structural and electrochemical features of the samples were investigated using X‐ray diffraction, electrochemical impedance spectroscopy, methanol crossover and direct methanol fuel cell (DMFC) tests. Highest conductivity values were obtained by using S–SnO2 as filler (0.094 S cm–1 at T = 110 °C and RH = 100%). The presence of the inorganic compound resulted in lower methanol crossover and improved DMFC performance with respect to a reference unfilled membrane. To improve the interface of the membrane electrode assembly (MEA), a layer of the composite electrolyte (i.e. the Nafion membrane containing 5 wt.‐% S–SnO2) was brushed on the electrodes, obtaining a DMFC operating at 110 °C with a power density (PD) of 100 mW cm–2 which corresponds to a PD improvement of 52% with respect to the unfilled Nafion membrane.
Prognostic value of heparanase expression and cellular localization in oral cancer
Authors: Leiser Yoav , Abu‐El‐Naaj Imad , Sabo Edmond , Akrish Sharon , Ilan Neta , Ben‐Izhak Ofer , Peled Micha , Vlodavsky Israel 
Published: September 2010

AbstractBackgroundMetastases formation depends on the ability of tumor cells to invade basement membranes in a process involving enzymes capable of degrading extracellular matrix components.MethodsWe examined the expression of heparanase in oral carcinomas and correlated its staining extent, intensity, and cellular localization with patients' outcome.ResultsQuantitative real‐time polymerase chain reaction (PCR) revealed over 4‐fold increase in heparanase levels in oral carcinomas compared to adjacent normal tissue. Normal oral epithelium was found negative for heparanase, while all oral carcinomas stained positively for heparanase. Heparanase staining was associated with Ki67 staining, a measure of cell proliferation. Notably, whereas cytoplasmic localization of heparanase was associated with high‐grade carcinomas, nuclear localization of the enzyme was found primarily in low‐grade, well‐differentiated tumors, and in all oral verrucous carcinomas.ConclusionExpression level and cellular localization of heparanase could serve as an important diagnostic marker in patients with oral cancer. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Effect of cup inclination angle during microseparation and rim loading on the wear of BIOLOX® delta ceramic‐on‐ceramic total hip replacement
Authors: Al‐Hajjar Mazen , Leslie Ian J. , Tipper Joanne , Williams Sophie , Fisher John , Jennings Louise M. 
Published: September 2010

AbstractCeramic‐on‐ceramic (CoC) bearings in total hip replacements (THRs) have shown low wear volumes under standard gait in hip simulator studies. However, clinical reports have indicated variations in wear rates and formation of stripe‐like wear area on the ceramic femoral heads. The aim of this study was to investigate the influence of cup inclination angle and microseparation on the wear of CoC bearings in THRs. The six station Leeds II Physiological Anatomical Joint Simulator was used to investigate the wear of 28 mm diameter alumina matrix composite ceramic bearings (BIOLOX®
delta). It was shown that increasing the cup inclination angle from 55o to 65o had no significant effect on the wear rate of BIOLOX®
delta CoC under both standard gait and microseparation conditions in this in vitro study. Under standard gait conditions, the mean wear rate for both cup inclination angle conditions was very low at 0.05 mm3/million cycles. The introduction of microseparation to the standard gait cycle increased the mean wear rates to 0.13 mm3/million cycles for the cup inclination angle of 55o and 0.11 mm3/million cycles for that of 65°. The level of increased wear with microseparation was not dependent on cup angle. A stripe of wear on the head also formed, with corresponding superior rim wear on the cup. The wear rates obtained were low compared to the HIPed third generation alumina ceramic (BIOLOX®
forte) tested under the same adverse conditions (1.84 mm3/million cycles). BIOLOX®
delta has shown lower wear than previous ceramic materials used in THR under adverse conditions. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010.
Characterization of hydroxyapatite‐perovskite (CaTiO3) composites: Phase evaluation and cellular response
Authors: Dubey Ashutosh Kumar , Tripathi Garima , Basu Bikramjit 
Published: September 2010

AbstractIn this study, an attempt was made to develop an understanding of the densification behavior, phase stability, and biocompatibility property of HA‐CaTiO3 biocomposite. The composites with varying CaTiO3 (40–80 wt %) content were sintered at temperatures ranging from 1200°C to 1500°C for 3–5 hr to establish optimum processing parameters. The phase analysis using spectral techniques indicate good thermochemical compatibility between HA and CaTiO3. The microstructural observations reveal homogeneous distribution of finer CaTiO3 phase (1–2 μm) along with coarser calcium phosphate phase. In vitro cell culture studies using L929 mouse fibroblast and SaOS2 human osteoblast cell lines provide clear evidence of cell adhesion, spreading, and proliferation as well as the formation of cellular bridges, and, hence, good in vitro biocompatibility of the developed composite can be realized. Also, the number of viable cells was found to increase with incubation period, as revealed by statistical analysis of the 3(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide assay data. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010.
Immunoenzymatic assay for the diagnosis of American tegumentary leishmaniasis using soluble and membrane‐enriched fractions from infectious Leishmania (Viannia) braziliensis
Authors: Cataldo Jamyra Iglesias , de Queiroz Mello Fernanda Carvalho , Mouta‐Confort Eliame , de Fátima Madeira Maria , de Oliveira Schubach Armando , da Silva Genestra Marcelo , Ribeiro Flávia Coelho , de Fátima Moreira‐Venâncio Célia , Passos Sônia Regina Lambert 
Published: September 2010

AbstractThe diagnosis of American tegumentary leishmaniasis (ATL) is based on the visualization or isolation of the parasite, which is a time‐consuming and poorly sensitive method. In this study, we evaluated the accuracy and reliability of ELISA for the diagnosis of ATL using soluble (SF) and membrane‐enriched (MF) antigen fractions obtained from an infectious strain of Leishmania (Viannia) braziliensis. A total of 152 serum samples investigated at a referral center in Rio de Janeiro, Brazil, between 2005 and 2007 were studied. Each sample was tested twice with each fraction for the calculation of reliability (intraclass coefficient (ICC)). Cut‐off values of 0.22 (SF) and 0.33 (MF) were defined. The use of the fractions resulted in good discrimination between patients, with a large area under the curve (P<0.0001), but no difference was observed between the two fractions (P=0.45). Sensitivity was 89.5% for each fraction, specificity was 89.5% for SF and 93.4% for MF, and the positive likelihood ratio was 8.5 for SF and 13.6 for MF. The ICCs were excellent (SF: 0.96 and MF: 0.90). The antigens tested provided precision and accuracy for the diagnosis of ATL, with SF being recommended due to its lower cost and greater practicality. J. Clin. Lab. Anal. 24:289–294, 2010. © 2010 Wiley‐Liss, Inc.
Nitrite in exhaled breath condensate as a marker of nitrossative stress in the airways of patients with asthma, COPD, and idiopathic pulmonary fibrosis
Authors: Rihák Vladimír , Zatloukal Petr , Chládková Jirina , Zimulová Alena , Havlínová Zuzana , Chládek Jaroslav 
Published: September 2010

AbstractBackground: Nitrite and nitrate are exhaled in droplets of an aerosol during breathing and can be assayed in the exhaled breath condensate (EBC) as markers of nitrossative stress in the airways of patients with asthma, COPD, and idiopathic pulmonary fibrosis (IPF). Subjects and methods: Using HPLC with fluorescence detection, nitrite and nitrate were assayed in EBC of 14 atopic patients with mild‐to‐moderate stable asthma, 18 atopic asthmatics with exacerbation, 14 COPD patients without exacerbation, 18 patients with exacerbated COPD, 13 patients with active IPF, and in 29 healthy subjects. Results: The geometric mean [exp(mean±SD)] EBC concentrations of nitrite (micromol/l) in patients with asthma [5.1(2.1–12.3)], exacerbation of asthma [5.1(2.8–9.6)], exacerbation of COPD [5.3(3.2–8.7)], and with IPF [5.5(2.9–10.2)] were higher (P<0.05) compared with those of healthy subjects [2.9(1.6–5.3)] and patients with stable COPD [3.0(1.3–6.7)]. Nitrite concentration increased with decreased lung function of patients with asthma (rs=−0.31, P<0.02). Presumably owing to the contamination of the EBC sample with nitrate during collection, nitrate levels were highly variable among healthy subjects and higher compared with all groups of patients. Conclusion: EBC nitrite is a suitable marker of nitrossative stress in adult patients with lung diseases but cannot differentiate controlled and exacerbated asthma. Further improvements to the methods of EBC collection and sample handling are warranted. J. Clin. Lab. Anal. 24:317–322, 2010. © 2010 Wiley‐Liss, Inc.
Elevated adenosine deaminase levels in celiac disease
Authors: Cakal Basak , Beyazit Yavuz , Koklu Seyfettin , Akbal Erdem , Biyikoglu Ibrahim , Yilmaz Gulsen 
Published: September 2010

AbstractCeliac disease (CD) is a genetically based chronic inflammatory disorder of the small bowel induced by the dietary gluten and possibly other environmental cofactors. The objective of this study was to investigate the relation of adenosine deaminase (ADA), a cytoplasmic enzyme involved in the catabolism of purine bases, as an index of altered immune response, with adult CD patients. ADA has been shown to increase in several inflammatory conditions, but there is no literature data indicating an alteration in CD. Serum levels of ADA were investigated in newly diagnosed 20 CD patients. ADA levels were compared in patients with CD and in healthy controls. Correlation analysis was also performed between ADA and other serum markers of CD (anti‐gliadin and anti‐endomysial antibodies) Mean serum ADA levels were significantly elevated in CD patients compared with control group. ROC curve analysis suggested that the optimum ADA level cut‐off point for CD was 12.27 U/l. At a cut‐off value of 12.27 U/l, the sensitivity was 80% and specificity was 100%. There was no statistically significant correlation between ADA and anti‐gliadin and anti‐endomisium antibodies. Serum ADA levels elevated significantly in CD patients, suggesting a partial role in activated T‐cell response in the disease pathophysiology. ADA can be used as a supportive diagnostic marker in patients with CD. J. Clin. Lab. Anal. 24:323–326, 2010. © 2010 Wiley‐Liss, Inc.
Evaluation of serum autoantibody levels in the diagnosis of ovarian endometrioma
Authors: Yi Yu‐Chiao , Wang Shih‐Chi , Chao Chun‐Chin , Su Chia‐Ling , Lee Yao‐Ling , Chen Ling‐Yun 
Published: September 2010

AbstractObjective: We analyzed autoantibodies against tumor‐associated antigens (TAAs) in the serum of patients with endometrioma and healthy controls to determine whether autoantibodies can be accurate biomarkers for the diagnosis of ovarian endometrioma.Methods: Serum samples were obtained from 56 patients with endometriosis and 66 healthy women who served as normal controls. The titers of antibodies against a panel of eight TAAs were analyzed using enzyme‐linked immunosorbent assay. Results: We found that the serum IGFII mRNA‐binding protein 1 (IMP1) autoantibody and cyclin B1 autoantibody could discriminate between healthy controls and endometriosis patients (AUC–ROC 0.777; 95% confidence interval [CI] 0.694–0.860, P<0.0005, and AUC–ROC 0.614; 95%confidence interval [CI] 0.513–0.714, P=0.031, respectively). Using 0.073 and 0.007 as the cutoff values for IMP1 and Cyclin B1 autoantibody, respectively, the sensitivity and specificity of IMP1 were 85.7 and 63.6%, respectively. When cylcin B1 was combined with IMP1, the specificity increased to 72.7% and the sensitivity slightly decreased to 83.9%. Conclusions: Our data suggest that IMP1 alone or combined with cyclin B1 seems to fulfill the requirements of sensitivity and specificity to become a useful clinical biomarker of endometrioma. However, further studies will be required to establish the predictive value and to support the clinical use of IMP1/cyclin B1 in the diagnosis and/or screening of endometriosis. J. Clin. Lab. Anal. 24:357–362, 2010. © 2010 Wiley‐Liss, Inc.
Glycation sites in neoglycoglycoconjugates from the terminal monosaccharide antigen of the O‐PS of Vibrio cholerae O1, serotype Ogawa, and BSA revealed by matrix‐assisted laser desorption–ionization tandem mass spectrometry
Authors: Jahouh Farid , Saksena Rina , Aiello Donatella , Napoli Anna , Sindona Giovanni , Kováč Pavol , Banoub Joseph H. 
Published: September 2010

AbstractWe present the MALDI‐TOF/TOF‐MS analyses of various hapten–bovine serum albumin (BSA) neoglycoconjugates obtained by squaric acid chemistry coupling of the spacer‐equipped, terminal monosaccharide of the O‐specific polysaccharide of Vibrio cholerae O1, serotype Ogawa, to BSA. These analyses allowed not only to calculate the molecular masses of the hapten–BSA neoglycoconjugates with different hapten–BSA ratios (4.3, 6.6 and 13.2) but, more importantly, also to localize the covalent linkages (conjugation sites) between the hapten and the carrier protein. Determination of the site of glycation was based on comparison of the MALDI‐TOF/TOF‐MS analysis of the peptides resulting from the digestion of BSA with similar data resulting from the digestion of BSA glycoconjugates, followed by sequencing by MALDI‐TOF/TOF‐MS/MS of the glycated peptides. The product‐ion scans of the protonated molecules were carried out with a MALDI‐TOF/TOF‐MS/MS tandem mass spectrometer equipped with a high‐collision energy cell. The high‐energy collision‐induced dissociation (CID) spectra afforded product ions formed by fragmentation of the carbohydrate hapten and amino acid sequences conjugated with fragments of the carbohydrate hapten. We were able to identify three conjugation sites on lysine residues (Lys235, Lys437 and Lys455). It was shown that these lysine residues are very reactive and bind lysine specific reagents. We presume that these Lys residues belong to those that are considered to be sterically more accessible on the surface of the tridimensional structure. The identification of the y‐series product ions was very useful for the sequencing of various peptides. The series of a‐ and b‐product ions confirmed the sequence of the conjugated peptides. Copyright © 2010 John Wiley & Sons, Ltd.
Resonant two‐magnon Raman scattering in high‐Tc cuprates
Authors: Kupčić I. 
Published: September 2010

AbstractResonance enhancement of one‐phonon, two‐phonon, and two‐magnon Raman scattering in a general, exactly solvable, multiband model is explained in a way that is in accordance with the general analytical properties of the total optical conductivity tensor. Using this approach, the charge‐transfer limit of the Emery three‐band model is examined to explain resonance enhancement of the two‐magnon Raman spectra of high‐Tc cuprates, which is found in experiments to be of 3 orders of magnitude. While previous Raman and optical conductivity analyzes of the cuprates, based on the single‐band Hubbard model, are found to be consistent with the picture where one hole per one CuO2 unit is localized on the Cu ion, the present three‐band approach allows the study of the opposite, strong copper‐oxygen hybridization limit, which is found to be in agreement with the results of nuclear magnetic resonance (NMR) and one‐phonon Raman scattering experiments. Copyright © 2010 John Wiley & Sons, Ltd.
Raman spectroscopy as a rapid screening method for ancient plain window glass
Authors: Baert K. , Meulebroeck W. , Wouters H. , Ceglia A. , Nys K. , Thienpont H. , Terryn H. 
Published: September 2010

AbstractThe aim of this paper is to highlight the potential of Raman spectroscopy as a fast screening method for large amounts of glass samples. Besides the dedicated research of specific glass collections during the last ten years, a broad corpus of archaeological window glass still needs to be investigated. For many sites, the amount of excavated glass artefacts is enormous and a selection of the most appropriate samples for chemical analysis is necessary.A classification can be made on the basis of the typical Raman signatures for the different kinds of glasses (alkali glass, high‐lime low‐alkali (HLLA) glass, Pb‐rich glass). Even in case of strong fluorescence, an uncomplicated treatment (normalisation) of the measured signals makes it possible to distinguish between the different types of glass. The preliminary screening of glass artefacts with Raman spectroscopy is a useful tool in the study of historical glass and can already answer some questions about provenance, period of production and authenticity of the glass samples. Copyright © 2010 John Wiley & Sons, Ltd.
Simultaneous capillary electrophoretic analysis of inorganic anions and cations in post‐blast extracts of acid–aluminum mixtures
Authors: Sarazin Cédric , Delaunay Nathalie , Varenne Anne , Costanza Christine , Eudes Véronique , Gareil Pierre 
Published: September 2010

AbstractBursts resulting from the chemical reaction between hydrochloric or nitric acid with aluminum foils are very often committed by the young delinquency in western countries because of its easiness of achievement. A fast, simple, selective, and cost‐effective method allowing the simultaneous detection of chloride and nitrate anions and aluminum(III) was thus required. This article focused on the development and validation of a CE method using a BGE containing 2,6‐pyridinedicarboxylic acid (PDC) acting as both an anionic chromophore and as an aluminum(III) complexing agent. First, the achievement of the speciation diagram of Al(III) in the presence of PDC allowed the choice of pH conditions for which aluminum(III) was globally anionic. The study of the selectivity for Al(III) in the presence of ten other cationic species potentially present in post‐blast residues dictated the choice of the PDC concentration at 20 mM. The validation step next demonstrated the figures of merit of the method, with an intermediate precision for Al(III) of 2% on normalized migration times and 3.5% on corrected areas. Finally, this method was used for analyses of real post‐blast extracts from acid–aluminum mixtures.
A simplified method for the determination of total homocysteine in plasma by electrospray tandem mass spectrometry
Authors: Persichilli Silvia , Gervasoni Jacopo , Iavarone Federica , Zuppi Cecilia , Zappacosta Bruno 
Published: September 2010

AbstractHyperhomocysteinemia is a risk factor for different diseases. Several methods have been developed to analyze homocysteine and the immunometric ones, although expensive, they are in widespread use. A rapid LC‐MS/MS method for homocysteine assay has been developed for the application of large clinical chemistry routines. Selected reaction monitoring was performed through the transitions m/z 136.0→90.1 for homocysteine and m/z 140.0→94.0 for the internal standard. ESI was used to generate [H+] adduct ions. Chromatographic isocratic separation was achieved using a strong cation exchange column. The mobile phase was methanol/water (20:80 v/v, containing 0.1% formic acid and 1.5 mmol/L ammonium formate in the water phase) at a flow rate of 0.250 mL/min (35°C). Samples treatment consisted in the reduction with DTT and deproteinization with methanol. Recovery, linearity, LOD, LOQ and total imprecision were evaluated to validate the method. Homocysteine values on 100 serum samples were compared with those obtained by HPLC and immunometric methods. The method is robust, selective and precise in the whole range of values studied. Moreover, low reagent cost and easiness of sample treatment make this method useful, not only for research, but also for routine work.