bluuebunny/crossref_metadata_2025_split huggingface dataset:
Whole-genome sequence of the strictly anaerobic bacterial strain SANA belonging to the family
Gottschalkiaceae
, isolated from a xenic culture of an anaerobic protist
Authors: Kondo Ryuji , Kataoka Takafumi 
Published: May 2024

ABSTRACT

An anaerobic bacterial strain SANA was isolated from a xenic culture of an anaerobic heterolobosean protist which was obtained from a saline lake in Japan. Its draft genome comprises 1 circular chromosome (3,490,293 bp), harboring 3,275 predicted protein-coding and 73 tRNA-encoding genes and 8 rRNA operons.
A retrospective study on intracranial mixed infection with tuberculous meningitis in Shenzhen, China
Authors: Fang Mutong , Li Sinian , Mao Zhi , Liu Xuhui , Wang Xiaomin , Lu Shuihua 
Published: May 2024

ABSTRACT

Tuberculous meningitis (TBM) is a prevalent global intracranial infection and the most lethal and disabling form of tuberculosis. TBM with mixed intracranial infections is clinically rare but has a higher mortality rate. To investigate the clinical characteristics of TBM with mixed intracranial infections, demographic and clinical data of TBM and pulmonary tuberculosis (PTB) patients admitted to Shenzhen Third People’s Hospital between January 2015 and October 2022 were collected anonymously. A total of 207 cases of TBM were diagnosed, of which 16 cases (7.73%) were TBM with mixed intracranial infections. The overall mortality rate of TBM cases was 16.4%, while the mortality rate of TBM cases with mixed intracranial infections was as high as 35.7%. Compared to simple TBM cases, TBM cases with mixed intracranial infections had severer clinical symptoms. The percentage of human immune deficiency virus (HIV)-positive TBM cases with mixed intracranial infections reached up to 68.8%. HIV co-infection, CD4
+
/CD8
+
T-cell counts less than 1, cranial nerve impairment, paralysis, cerebral infarction, PRO less than 450 mg/L, WBC less than 10 × 10
6
/L, and CL more than 120 mmol/L were risk factors for TBM cases with mixed intracranial infections. Compared to PTB, HIV co-infection, CD4
+
T cell less than 550 /uL, and age less than 45 years were risk factors for TBM, and TBM was associated with higher mortality rates. Our study provides additional data to better understand single TBM and TBM with mixed intracranial infections. More than two-thirds of TBM cases with mixed intracranial infections were HIV-positive. Clinicians should consider the possibility of multiple infections in people with TBM/HIV co-infection.

IMPORTANCE
TBM can cause severe neurological damage and death, and TBM with mixed intracranial infections can exacerbate the damage and poor prognosis of the disease. TBM with mixed intracranial infections is a rare disease, which has led to an incomplete understanding of its clinical features. This study investigated the clinical features of TBM and its associated factors by comparing the characteristics of TBM with mixed intracranial infections, single TBM and pulmonary tuberculosis. This information will help to improve the understanding of TBM, diagnostic accuracy and treatment outcomes.
Supplemental Material: Triassic granitic magmatism in the Lancangjiang zone in southwestern China associated with Paleo-Tethys evolution and its control on tin mineralization
Authors: Zheng Mingjun , al. et 
Published: May 2024

<p>Table S1: Zircon U-Pb isotopic data for the Daguanshan granites. Table S2: Cassiterite U-Pb isotopic data for the Hongmaoling deposit. Table S3: Trace elements (ppm), <em>T</em><sub>zr</sub> (°C), and ΔFMQ values of zircons from the Daguanshan granites and Triassic tin-mineralized granite in Peninsular Malaysia. Table S4: Major (wt%) and trace elements (ppm) of the Daguanshan granites. Table S5: Whole-rock Sr-Nd isotopic compositions of the Daguanshan granites. Table S6: Zircon Hf isotopes from the Daguanshan hornblende-biotite granite and muscovite granite. </p>
Obtaining Plasma–Dust Clouds from Meteoritic Matter, its Analogs and Simulants of Lunar Regolith Using Microwave Discharge
Authors: Borzosekov V. D. , Akhmadullina N. S. , Sokolov A. S. , Gayanova T. E. , Rezaeva A. D. , Stepakhin V. D. , Konchekov E. M. , Malakhov D. V. , Voronova E. V. , Nugaev I. R. , Logvinenko V. P. , Knyazev A. V. , Letunov A. A. , Kharlachev D. E. , Obraztsova E. A. , Morozova T. I. , Zaitsev M. A. , Ishchenko A. V. , Weinstein I. A. , Grohovsky V. I. , Shishilov O. N. , Skvortsova N. N. 
Published: May 2024

Abstract—
In the experiment, plasma–dust clouds were obtained from the substance of the Tsarev meteorite, a simulant of lunar regolith LMS-1D and ilmenite concentrate using a microwave discharge in powder media. For each of the samples, the dynamics of the development of the discharge and the formation of a plasma–dust cloud with subsequent relaxation after the end of the microwave pulse were recorded. From the emission spectra of the plasma and the surface of a solid body, the temperatures of the gas, electrons and surface were determined. A comparison of the phase and elemental composition of the initial samples and samples after exposure to plasma showed that there is no significant change in the composition. However, scanning electron microscopy results clearly indicate spheroidization of the original angular and irregularly shaped particles. The appearance of spherical particles is also observed, the dimensions of which are larger than the linear dimensions of the particles in the original sample. The results obtained indicate the possibility of using such experiments to study chemical and plasma-chemical processes of synthesis and modification of substances under conditions of plasma–dust clouds encountered in space phenomena.
Automatic detection of cardiac conditions from photos of electrocardiogram captured by smartphones
Authors: Wong Chun-Ka , Lau Yuk Ming , Lui Hin Wai , Chan Wai Fung , San Wing Chun , Zhou Mi , Cheng Yangyang , Huang Duo , Lai Wing Hon , Lau Yee Man , Siu Chung Wah 
Published: May 2024

Background
Researchers have developed machine learning-based ECG diagnostic algorithms that match or even surpass cardiologist level of performance. However, most of them cannot be used in real-world, as older generation ECG machines do not permit installation of new algorithms.

Objective
To develop a smartphone application that automatically extract ECG waveforms from photos and to convert them to voltage-time series for downstream analysis by a variety of diagnostic algorithms built by researchers.

Methods
A novel approach of using objective detection and image segmentation models to automatically extract ECG waveforms from photos taken by clinicians was devised. Modular machine learning models were developed to sequentially perform waveform identification, gridline removal, and scale calibration. The extracted data were then analysed using a machine learning-based cardiac rhythm classifier.

Results
Waveforms from 40 516 scanned and 444 photographed ECGs were automatically extracted. 12 828 of 13 258 (96.8%) scanned and 5399 of 5743 (94.0%) photographed waveforms were correctly cropped and labelled. 11 604 of 12 735 (91.1%) scanned and 5062 of 5752 (88.0%) photographed waveforms achieved successful voltage-time signal extraction after automatic gridline and background noise removal. In a proof-of-concept demonstration, an atrial fibrillation diagnostic algorithm achieved 91.3% sensitivity, 94.2% specificity, 95.6% positive predictive value, 88.6% negative predictive value and 93.4% F1 score, using photos of ECGs as input.

Conclusion
Object detection and image segmentation models allow automatic extraction of ECG signals from photos for downstream diagnostics. This novel pipeline circumvents the need for costly ECG hardware upgrades, thereby paving the way for large-scale implementation of machine learning-based diagnostic algorithms.
CHD2 Regulates Neuron–Glioma Interactions in Pediatric Glioma
Authors: Zhang Xu , Duan Shoufu , Apostolou Panagiota E. , Wu Xiaoping , Watanabe Jun , Gallitto Matthew , Barron Tara , Taylor Kathryn R. , Woo Pamelyn J. , Hua Xu , Zhou Hui , Wei Hong-Jian , McQuillan Nicholas , Kang Kyung-Don , Friedman Gregory K. , Canoll Peter D. , Chang Kenneth , Wu Cheng-Chia , Hashizume Rintaro , Vakoc Christopher R. , Monje Michelle , McKhann Guy M. , Gogos Joseph A. , Zhang Zhiguo 
Published: May 2024

Abstract
High-grade gliomas (HGG) are deadly diseases for both adult and pediatric patients. Recently, it has been shown that neuronal activity promotes the progression of multiple subgroups of HGG. However, epigenetic mechanisms that govern this process remain elusive. Here we report that the chromatin remodeler chromodomain helicase DNA-binding protein 2 (CHD2) regulates neuron–glioma interactions in diffuse midline glioma (DMG) characterized by onco-histone H3.1K27M. Depletion of CHD2 in H3.1K27M DMG cells compromises cell viability and neuron-to-glioma synaptic connections in vitro, neuron-induced proliferation of H3.1K27M DMG cells in vitro and in vivo, activity-dependent calcium transients in vivo, and extends the survival of H3.1K27M DMG-bearing mice. Mechanistically, CHD2 coordinates with the transcription factor FOSL1 to control the expression of axon-guidance and synaptic genes in H3.1K27M DMG cells. Together, our study reveals a mechanism whereby CHD2 controls the intrinsic gene program of the H3.1K27M DMG subtype, which in turn regulates the tumor growth-promoting interactions of glioma cells with neurons.
Significance: Neurons drive the proliferation and invasion of glioma cells. Here we show that chromatin remodeler chromodomain helicase DNA-binding protein 2 controls the epigenome and expression of axon-guidance and synaptic genes, thereby promoting neuron-induced proliferation of H3.1K27M diffuse midline glioma and the pathogenesis of this deadly disease.
Molecular Pathways and Cellular Subsets Associated with Adverse Clinical Outcomes in Overlapping Immune-Related Myocarditis and Myositis
Authors: Siddiqui Bilal A. , Palaskas Nicolas L. , Basu Sreyashi , Dai Yibo , He Zhong , Yadav Shalini S. , Allison James P. , Sheth Rahul A. , Tummala Sudhakar , Buja Maximilian , Bhattacharjee Meenakshi B. , Iliescu Cezar , Rawther-Karedath Anishia , Deswal Anita , Wang Linghua , Sharma Padmanee , Subudhi Sumit K. 
Published: May 2024

Abstract
Immune checkpoint therapies (ICT) can induce life-threatening immune-related adverse events, including myocarditis and myositis, which are rare but often concurrent. The molecular pathways and immune subsets underlying these toxicities remain poorly understood. To address this need, we performed single-cell RNA sequencing of heart and skeletal muscle biopsies obtained from living patients with cancers treated with ICTs and admitted to the hospital with myocarditis and/or myositis (overlapping myocarditis plus myositis, n = 10; myocarditis-only, n = 1) or ICT-exposed patients ruled out for toxicity utilized as controls (n = 9). All biopsies were obtained within 96 hours of clinical presentation. Analyses of 58,523 cells revealed CD8+ T cells with a cytotoxic phenotype expressing activation/exhaustion markers in both myocarditis and myositis. Furthermore, the analyses identified a population of myeloid cells expressing tissue-resident signatures and FcγRIIIa (CD16a), which is known to bind IgG and regulate complement activation. Immunohistochemistry of affected cardiac and skeletal muscle tissues revealed protein expression of pan-IgG and complement product C4d, which were associated with the presence of high-titer serum autoantibodies against muscle antigens in a subset of patients. We further identified a population of inflammatory IL1B+TNF+ myeloid cells specifically enriched in myocarditis and associated with greater toxicity severity and poorer clinical outcomes. These results provide insight into the myeloid subsets present in human immune-related myocarditis and myositis tissues and nominate new targets for investigation into rational treatments to overcome these high-mortality toxicities.
See related Spotlight by Fankhauser et al., p. 954
Behavioral Problems Associated with Digital Screen Exposure in Children Aged 2–5 Years: Cross-sectional Study
Authors: Kaur Nimran , Gupta Madhu , Kiran Tanvi , Malhi Prahbhjot , Grover Sandeep 
Published: May 2024

Objective: To assess the emotional problems associated with digital-screen exposure (DSE) among 2–5-year-old children in Chandigarh, North India. Methods: A cross-sectional study was conducted among 400 randomly selected families with children aged 2–5 years using the validated DSE questionnaire and the standard preschool-child behavior checklist from October 2017 to March 2018. The American (2016) and Indian (2020) Academy of Pediatrics guidelines were followed to define excessive DSE. To estimate the probability for the predictors of emotional problems with DSE, we performed the per-binary multivariate logistic regression model, giving an adjusted odds ratio (AOR), using IBM SPSS Statistics for Macintosh, Version 25.0. Results: The mean age of the children was 3.5 years (standard deviation = 0.9). Nearly 15% of children were with borderline/clinical cases of emotional problems. The significant protective factors for individual emotional problems among children were watching learning media content [Oppositional defiant disorder (AOR = 0.39 CI 0.22, 0.72, p = 0.002)], having DSE less than one hour [Attention deficit hyperactivity disorder (AOR = 0.19 CI 0.08, 0.43, p<0.0001)], presence of media rules at home [Sleep problems (AOR = 0.03 CI 0.001, 0.69, p = 0.028)], age of initiating DSE before two years [Total emotional problems (AOR = 0.5, 0.22, 0.93, p = 0.03)], and caregivers DSE of less than two hours per day [Depression (AOR = 0.04 CI 0.002, 0.75, p = 0.031)]. While the significant predictors were playing outdoors almost every day [Total emotional problems (AOR = 2.2 CI 1.06, 4.78, p = 0.034)], average activity of less than 30 minutes per day [Total emotional problems (AOR = 2.4 CI 1.22, 4.73, p = 0.01)], boys [Total emotional problems (AOR = 2 CI 1.08, 3.55, p = 0.028)], children aged less than 3.5 years [Total emotional problems (AOR = 1.9 CI 1.05, 3.59, p = 0.03)] and higher socio-economic status [Withdrawn (AOR = 5 CI 1.19, 20.97, p = 0.028)]. Conclusion: There was a moderate prevalence of emotional problems among children with excessive DSE. Specific emotional problems were significantly associated with the DSE among children.
Anaesthetic Management for a Dilated Cardiomyopathy Patient, Scheduled for Lumbar Spine Surgery
Authors: Srivastava Vinit K. , Gangireddy Hareendranath , Hamsa Vellore Srikanth , Krishna Yethirajyam Rama 
Published: May 2024

Background: Dilated cardiomyopathy (DCM) is characterized by dilatation and impaired contraction of left or both ventricles. Diagnostic criteria are left ventricular ejection fraction less than 40% or fractional shortening less than 25%. Perioperative anesthetic management of these patients are very challenging for anesthesiologists and intensive care team. Case presentation: A 55-year-old female, presented with complaints of severe low-back ache with bilateral lower limb involvement without involvement of bladder and bowel. Severe cord compression at the L4-5 level and severe lumbar canal stenosis were detected by magnetic resonance imaging. Echocardiography showed that the left ventricle (LV) had global hypokinesia, with an ejection fraction of 25% to 30%. The planed surgical procedures were L4 lumbar laminectomy and L4-5 microdiscectomy. Conclusion: Continuous monitoring and control of hemodynamic parameters as well as central venous pressure guided fluid therapy is the cornerstone in improving outcome in patients with dilated cardiomyopathy for non-cardiac surgery.
The Inequality in Healthcare Access in Bihar: Pattern and Determinants
Authors: Ahmed Shakeel 
Published: May 2024

Healthcare access varies across socioeconomic determinants and therefore, states’ demographic and socioeconomic structure plays an important role in an individual’s health status in the state. For example, richer have better access to healthcare than poorer, and women have lower healthcare access than men. In this study, the role of socioeconomic determinants in healthcare access is discussed in five states of India—Jharkhand, Uttar Pradesh, Odisha and Madhya Pradesh; therefore, this article aims to determine how healthcare access across socioeconomic determinants varies across states. The study is based on the National Sample Survey 75th round of data on health (2017–2018). A comparative study is conducted to know the percentage of non-access to healthcare (unmet needs) across different socioeconomic determinants such as age, gender, income, religion, caste and others. A logistic regression model is used to determine significant socioeconomic factors in healthcare access in all five states. The concentration index and concentration curve show the inequality in healthcare access across income quintiles. The result shows that Bihar has the highest cases of non-access to healthcare across most socioeconomic determinants followed by Odisha and Jharkhand. In addition, cases of non-access to healthcare in all five states are largely higher than the national average. The result further shows that only a few socioeconomic determinants such as income class and caste are significant to healthcare access and state-wise, they vary. JEL: I140; I150; I18
Clinical evaluation and determinants of response to HBI0101 (BCMA CART) therapy in relapsed/refractory multiple myeloma
Authors: Kfir-Erenfeld Shlomit , Asherie Nathalie , Lebel Eyal , Vainstein Vladimir , Assayag Miri , Dubnikov Sharon Tatyana , Grisariu Sigal , Avni Batia , Elias Shlomo , Alexander-Shani Rivka , Bessig Nomi , Shehadeh Alaa , Ishtay Aseel , Zelmanovich Veronica , Zimran Eran , Pick Marjorie , Roziner Ilan , Kenett Ron S. , Cohen Yael , Avivi Irit , Cohen Cyrille J. , Gatt Moshe E. , Stepensky Polina 
Published: May 2024

Abstract
HBI0101 is an academic chimeric antigen receptor T-cell (CART)–targeted to B-cell maturation antigen (BCMA) for the treatment of relapsed and refractory multiple myeloma (R/RMM) and light chain amyloidosis. Herein, we present the phase 1b/2 results of 50 heavily pretreated patients with R/RMM dosed with 800 × 106 CART cells. Inclusion criteria were relatively permissive (i.e., performance status and baseline organ function) and consequently, approximately half of the enrolled patients would have been ineligible for pivotal clinical trials. The median time elapsed from patient enrollment until CART delivery was 25 days (range, 14-65). HBI0101-related toxicities included grade 1 to 3 cytokine release syndrome, grade 3 to 4 hematologic toxicities, and grade 1 to 2 immune effector cell–associated neurotoxicity syndrome. Responses were achieved in 90% of the patients, 56% achieved stringent and complete response, and 70% reached a minimal residual disease negativity. Within a median follow-up of 12.3 months, the median progression-free survival (PFS) was 11.0 months (95% confidence interval [CI], 6.2-14.6), and the overall survival was not reached (95% CI, 13.3 to not reached). Multivariable analysis on patient/disease and CART-related characteristics revealed that high-risk cytogenetic, extramedullary disease, and increased number of effector-memory T cells in CART products were independently associated with inferior PFS. In conclusion, comprehensive analyses of the parameters affecting the response to CART therapy are essential for improving patients’ outcome. This trial was registered at www.ClinicalTrials.gov as #NCT04720313.
Experimental Study on the Electrochemical Performance of PEMFC under Different Assembly Forces
Authors: Su Tongze , Liu Jiaran , Wei Yanqiang , Li Yihuizi , Luo Weichao , Tan Jinzhu 
Published: May 2024

AbstractProton exchange membrane fuel cell (PEMFC) is of paramount significance to the development of clean energy. The components of PEMFC are assembled using many pairs of nuts and bolts. The assembly champing bolt torque is critical to the electrochemical performance and mechanical stability of PEMFC. In this paper, a PEMFC with the three-channel serpentine flow field was used and studied. The different assembly clamping bolt torques were applied to the PEMFC in three uniform assembly bolt torque and six non-uniform assembly bolt torque conditions, respectively. And then, the electrochemical performance experiments were performed to study the effect of the assembly bolt torque on the electrochemical performance. The test results show that the assembly bolt torque significantly affected the electrochemical performance of the PEMFC. In uniform assembly bolt torque conditions, the maximal power density increased initially as the assembly bolt torque increased, and then decreased on further increasing the assembly torque. It existed the optimum assembly torque which was found to be 3.0 N·m in this work. In non-uniform assembly clamping bolt torque conditions, the optimum electrochemical performance appeared in the condition where the assembly torque of each bolt was closer to be 3.0 N·m. This could be due to the change of the contact resistance between the gas diffusion layer and bipolar plate and mass transport resistance for the hydrogen and oxygen towards the catalyst layers. This work could optimize the assembly force conditions and provide useful information for the practical PEMFC stack assembly.
The quality of reporting in case reports of permanent neonatal diabetes mellitus: a cross-sectional study
Authors: Jia Pengli , Wang Ling , Yang Xi , Pei WenTing , Xu Chang , Feng Jinglin , Han Ying 
Published: May 2024

Abstract
Background
Although randomized trials and systematic reviews provide the best evidence to guide medical practice, many permanent neonatal diabetes mellitus (PNDM) studies have been published as case reports. However, the quality of these studies has not been assessed. The purpose of this study was to assess the extent to which the current case reports for PNDM comply with the Case Report (CARE) guidelines and to explore variables associated with the reporting.

Method
Six English and four Chinese databases were searched from their inception to December 2022 for PNDM case reports. The 23 items CARE checklist was used to measure reporting quality. Primary outcome was the adherence rate of each CARE item and second outcome was total reporting score for each included PNDM case report. Linear and logistic regression analyses were used to examine the connection between five pre-specified predictor variables and the reporting quality. The predictor variables were impact factor of the published journal (<3.4 vs. ≥3.4, categorized according to the median), funding (yes vs. no), language (English vs. other language), published journal type (general vs. special) and year of publication (>2013 vs. ≤ 2013).

Result
In total, 105 PNDM case reports were included in this study. None of the 105 PNDM case reports fulfilled all 23 items of the CARE checklist. The response rate of 11 items were under 50%, including prognostic characteristics presentation (0%), patient perspective interpretation (0%), diagnostic challenges statement (2.9%), clinical course summary (21.0%), diagnostic reasoning statement (22.9%), title identification (24.8%), case presentation (33.3%), disease history description (34.3%), strengths and limitations explanation (41.0%), informed consent statement (45.7%), and lesson elucidation (47.6%). This study identified that the PNDM case reports published in higher impact factor journals were statistically associated with a higher reporting quality.

Conclusion
The reporting of case reports for PNDM is generally poor. As a result, this information may be misleading to providers, and the clinical applications may be detrimental to patient care. To improve reporting quality, journals should encourage strict adherence to the CARE guidelines.