bluuebunny/crossref_metadata_2025_split huggingface dataset:
Issues in Environmental Epidemiological Research: The Example of Environmental Lead and Health
Authors: McMichael A. J. 
Published: June 2011

Modern environmental epidemiology encompasses the “traditional” area of physico-chemical hazards, along with health hazards in the societal environment (e.g. noise, stress, social organisation), and, increasingly, supranational problems (e.g. ozone depletion, global warming). As governments undertake environmental management, improved quantitative estimates of environmental risks to health are needed. Methodological difficulties of environmental epidemiological research include problems of exposure measurement, of estimating exposure at the level of the individual, and of detecting relatively small effects (particularly at low exposure levels). The health hazards of occupational lead exposure are well documented. The health hazards of environmental exposure to lead, within the general population, remain a focus of continuing epidemiological research. Indeed, the reported adverse effects upon the developing central nervous system of young children are now central to public health debate about environmental lead exposure standards. Recent evidence from cohort studies in several countries indicates adverse effects of environmental lead exposure upon early childhood mental development. In South Australia, a cohort study of children born in a lead smelter community, Port Pirie, has revealed evidence of such an effect. After controlling for many potential confounding factors (social, behavioural, family, and medical), cumulative postnatal lead exposure was found to be weakly associated with an adverse effect upon mental development at age two years and, more strongly, at age four years. The relations between environmental epidemiological research and public health policy are discussed.
Patterns of Pesticide Usage by Cereal Crop Farmers in Western Australia
Authors: Spickett JT , Dolin PJ , Phillips MR , Priestley CJM 
Published: June 2011

In Western Australia there has been an increase in the use of herbicides in recent years due to a change in farming practices. This change, together with more general public concern over exposure to chemicals, has resulted in farmers expressing concern over the possible long term health effects from exposure to herbicides. As part of a long term study of the possible health effects from such exposure, a survey was carried out to establish the extent of pesticide use within the cereal farming community of Western Australia. Of the 9, 408 properties surveyed, 2, 921 responses were received which represents a 32.2% response rate. The results indicate that a wide range of chemicals are used as insecticides, fumigants, seed dressings, seed pickles, herbicides, and rodent poisons. At the time of the survey in 1985, products containing prespruf and 1, 1, 1-trichloro-2, 2-bis (p-chlorophenyl)ethane (DDT) were the most popular insecticide, and products containing diquat, diclofop-methyl, chlorsulfuron and glyphosate as activeingredients represented the four most popular herbicides.
The Interaction of Lead Exposure and Pregnancy
Authors: O'Halloran K , Spickett JT 
Published: June 2011

The toxic effects of low-level lead exposure have been the subject of a good deal of research and media attention in recent times. In most countries, the acceptable occupational exposure limit for lead is being progressively decreased as the adverse health effects of lead are being identified at levels approaching those found in non-occupational environments. Due to the sensitive nature of the fetus to hazardous substances, the exposure to lead of the unborn child via maternal sources is of critical concern. Preterm delivery, congenital abnormalities and decreases in growth stature have all been associated with prenatal lead exposure at “acceptable” levels. There is an accumulation of evidence which indicates that maternal exposures prior to conception can play an important role in determining blood lead levels during pregnancy. In light of these observations the practice of removing the pregnant woman from lead sources may be of questionable value with regards to providing sufficient protection for the fetus. This article reviews the relevant literature pertaining to the mobilization of lead from bone during pregnancy and the toxicity of low-level lead exposure to the fetus, and briefly discusses some factors which may affect this toxicity.
Health Practices and Indices of a Poor Urban Population in Indonesia Part I: Patterns of Health Service Utilization
Authors: Kaye Katherine , Novell Michael K 
Published: June 2011

This first section in a two-part study of health indices and practices among residents living in a Jakarta slum describes the use of public and private primary health care services in relation to socioeconomic and health status. As problems associated with urban poverty rapidly increase in developing countries, it is important to study the ethnic and economic diversity which exists in slums and shanty towns: results of such studies should inform the development of effective strategies for outreach and service delivery. Through a survey of 690 mothers and 593 children, we found that 1) poorer residents were more likely than relatively affluent ones to rely on local government clinics ( posyandus) for primary health care; 2) regular posyanduusers were more likely than non-users to be fully immunized and to use ORT correctly; 3) delivery in hospital w as common among all residents, but especially among the more affluent; and 4) prevalence of contraception was high and not associated with socio-economic status or type of primary health care service used. Strengthening primary health care services at the government's local health posts could benefit all groups in the community if wealthier residents participated more in the posyandus. Standards of care in the private sector should also be improved. Asia Pac J Public Health 1994;7(3):178-82.
Flow Volume Curves as Screening Tests for Early Effects of Smoking in Asymptomatic Children and Young Adults
Authors: Milaat Waleed A , El-Gamal Fathi M , Ghabra Tawfik M 
Published: June 2011

The objectives of this study were to investigate the early effects of smoking on ventilation function of the lungs of children and young adults, and to determine the suitable lung function tests that can be used for screening of this effect. Medical and personal questionnaires, spirometric tests for twelve different lung function indices as well as anthropometric measurements were conducted on 538 randomly selected asymptomatic subjects (134 smokers, 404 non-smokers). Smokers had significantly lower values for peripheral airway lung function tests compared to non-smokers and this is evident within a few years of initiating smoking. The number of cigarettes smoked per day was also significantly associated with decrease to these lung function parameters. Lung function tests suitable for screening were identified. The number of cigarettes and duration of smoking seem to interact to increase the insult on the peripheral airway function in children and young adults suggesting an irritant effect, probably mediated through an airway inflammation which starts at the periphery of the lungs. Lung function tests used here can be the early indicators of early changes in asymptomatic children and young adults and can be a valuable screening tool.
Linking Public Health Education And Practice: The Australian Experience
Authors: Rotem Arie , Hine Bronwyn , Hodgkinson Alan 
Published: June 2011

The public health workforce in Australia is highly skilled, multifunctional, and drawn from a variety of backgrounds, including clinical practice and non-health areas. A wide range of activities is needed to meet the educational and training requirements of this workforce, including on the job inservice training, context specific continuing education programs and short courses, distance and self-directed learning packages, and postgraduate University level courses. The core components of public health today include: a social and political commitment to health, a shared responsibility between government and the public, and a multidisciplinary field of action. The challenge for those providing education and training for the public health workforce is to ensure graduates have the broad range of knowledge and skills needed in this climate. A system-wide approach to learning, where knowledge and skill development is related to the practices and settings of service and program delivery, will ensure strong links between education and practice.
Effects of Cigarette Smoking on Lung Function of Saudi Students
Authors: Milaat Waleed A. , EI-Ganai Fathim M. 
Published: June 2011

This study was carried out to investigate the association between cigarette smoking and state of lung function among Saudi male students. A cross sectional study was conducted on a group of 680 male Saudi students. Detailed personal questionnaire, British Medical Research Council questionnaire on respiratory symptoms and smoking habit, were administered and forced spirometry and anthropometric measurements were conducted on them. Multiple regression models were used to study the cumulative effect of smoking on the function of the lung of smoking students. The percentage of cigarette smoking among the studied group was 20.29%. Smoking students had significantly lower values for lung function tests (FEV1%, FEF200-1200, FEF25-75, FEF75-85, and PEFR) compared to non-smokers. After controlling for other confounding variables, increased duration of smoking and number of cigarette smoked per day were significantly associated with more limitation of airflow in airways of the smoking young students. Recommendations were discussed accordingly.
Influence of Low Birth Weight on the Intellectual Performance of Children at Entry to First Year of School
Authors: Dharmage Shyamali , Fernando Dulitha N. 
Published: June 2011

Influence of low birth weight (LBW) on children at entry to school was studied by comparing a group of LBW children with a group of normal birth weight (NBW) children. A wide variety of study instruments was used to obtain required information and steps were taken to ensure quality of data. Influence of LBW on competency at entry to school was examined while accounting for the possible confounders of the relevant association. LBW group was found to have a lower level of intellectual performance at the time of school entry. The significant factors influencing this association were: poor socio-economic status, lower duration of pre-school education, lack of availability of reading material and lower nutritional status. However the influence of LBW on performance at entry to school remained significant once the above confounders were controlled. This highlights the need to prevent LBW and to maximize the intellectual ability of LBW survivors by appropriate interventions.
Lead in diagenetic pyrite: evidence for Pb-tolerant bacteria in a red-bed Cu deposit, Quebec Appalachians, Canada
Authors: Cabral A. R. , Beaudoin G. , Munnik F. 
Published: June 2011

AbstractDiagenetic pyrite from the Silurian continental red bed-hosted Transfiguration cupriferous deposit in the Quebec Appalachians, Gaspe Belt, Canada, contains up to ∼2% (m/m) Pb. This large Pb content in pyrite contrasts with experimental determinations that indicate solubility of <0.1% (m/m) PbS in pyrite at high temperature. The distribution of Pb in pyrite is heterogeneous, with plumbiferous domains occurring as patches and concentric growth layers alternating with Mn- and Mo-bearing zones. The plumbiferous pyrite is surrounded by As- and Cu-rich rims. This compositional heterogeneity, however, is elusive under normal backscattered-electron (BSE) imaging, but it can be recognized under high-gain BSE. Proton-induced X-ray emission (PIXE) confirms the presence of Pb. Plumbiferous pyrite with >0.1% (m/m) Pb has rarely been described; it is thus possible that plumbiferous pyrite may have been overlooked in metalliferous deposits worldwide. The plumbiferous pyrite from Transfiguration has a light S-isotope composition that is characteristic of bacterial sulphate reduction. We suggest that Pb in diagenetic pyrite indicates Pb-tolerant bacterial activity and, perhaps, constitutes a biosignature of bacterial tolerance to Pb in ancient sedimentary systems.
Holtite and dumortierite from the Szklary Pegmatite, Lower Silesia, Poland
Authors: Pieczka A. , Grew E. S. , Groat L. A. , Evans R. J. 
Published: June 2011

AbstractThe Szklary holtite is represented by three compositional varieties: (1) Ta-bearing (up to 14.66 wt.% Ta2O5), which forms homogeneous crystals and cores within zoned crystals; (2) Ti-bearing (up to 3.82 wt.% TiO2), found as small domains within the core; and (3) Nb-bearing (up to 5.30 wt.% Nb2O5,) forming the rims of zoned crystals. All three varieties show variable Sb+As content, reaching 19.18 wt.% Sb2O3 (0.87 Sb a.p.f.u.) and 3.30 wt.% As2O3 (0.22 As a.p.f.u.) in zoned Ta-bearing holtite, which constitutes the largest Sb+As content reported for the mineral. The zoning in holtite is a result of Ta-Nb fractionation in the parental pegmatite-forming melt together with contamination of the relatively thin Szklary dyke by Fe, Mg and Ti. Holtite and the As- and Sb-bearing dumortierite, which in places overgrows the youngest Nb-bearing zone, suggest the following crystallization sequence: Ta-bearing holtite → Ti-bearing holtite → Nb-bearing holtite → As- and Sb-bearing, (Ta,Nb,Ti)-poor dumortierite → As- and Sb-dominant, (Ta,Nb,Ti)-free dumortierite-like mineral (16.81 wt.% As2O3 and 10.23 wt.% Sb2O3) with (As+Sb) > Si. The last phase is potentially a new mineral species. Al6□☐(Sb,As)3O15, or Al5☐2B(Sb,As)3O12(OH)3, belonging to the dumortierite group. The Szklary holtite shows no evidence of clustering of compositions around ‘holtite I’ and ‘holtite II'. Instead, the substitutions of Si4+ by Sb3++As3+ at the Si/Sb sites and of Ta5+ by Nb5+ or Ti4+ at the Al(l) site suggest possible solid solutions between: (1) (Sb,As)-poor and (Sb,As)-rich holtite; (2) dumortierite and the unnamed (As+Sb)-dominant dumortierite-like mineral; and (3) Ti-bearing dumortierite and holtite. i.e. our data provide further evidence for miscibility between holtite and dumortierite, but leave open the question of defining the distinction between them. The Szklary holtite crystallized from the melt along with other primary Ta-Nb-(Ti) minerals such as columbite-(Mn), tantalite-(Mn), stibiotantalite and stibiocolumbite as the availability of Ta decreased. The origin of the parental melt can be related to anatexis in the adjacent Sowie Mountains complex, leading to widespread migmatization and metamorphic segregation in pelitic-psammitic sediments metamorphosed at ∼390—380 Ma.
The crystal structure of perloffite
Authors: Elliott P. , Willis A. C. 
Published: June 2011

AbstractThe structure of perloffite, ideally BaMn22+Fe23+(PO4)3(OH)3, has been solved and refined from single-crystal X-ray data collected on a Sr-bearing sample from the Spring Creek copper mine, near Wilmington, South Australia. A fragment of the crystal used for the collection of the X-ray intensity data was analysed by electron microprobe. The resulting unit formula is (Ba1.00,Sr0.03Σ1.03) (Mn2+1.15,Fe2+0.76Ca0.05Mg0.02,Na0.01)Σ1.99(Fe3+1.94,Al0.06)Σ2.00(PO4)2.99(OH)3.04. The structure is monoclinic. space group P21/m, a = 9.2425(18), b = 12.470(3), c = 5.002(1) Å, 0 = 100.19(3)°, V = 567.41(19) Å3, Z = 2, and was refined to R1 = 4.71% for 1568 unique observed reflections. The X site is [11]-coordinated and is occupied by dominant Ba and minor Sr, with an <X—O< distance of 2.953 Å. The Ml site is octahedrally coordinated and is occupied by Mn2+and Fe2+ plus minor Ca, Mg and Na with an observed <M1—O> distance of 2.208 Å. The M2 site is also octahedrally coordinated and is occupied by Fe3+ plus minor Al with a <M2—O> distance of 2.018 A. Perloffite is isostructural with other members of the bjarebyite group. Edge-linked [M22O6(OH)4] dimers link via corners to form chains along [010]. Chains are decorated with PO4 tetrahedra and link in the a direction via [M1O5(OH)] octahedra and (Ba,Sr) atoms to form a complex framework. The H2 atom hydrogen-bonds strongly to the O3 anion, providing additional linkage between chains.
Thermal expansion of andalusite and sillimanite at ambient pressure: a powder X-ray diffraction study up to 1000°C
Authors: Hu X. , Liu X. , He Q. , Wang H. , Qin S. , Ren L. , Wu C. M. , Chang L. 
Published: June 2011

AbstractThe unit-cell parameters of andalusite and sillimanite have been measured by high-Tpowder X-ray diffraction up to 1000°C at ambient pressure. Within the temperature range investigated, all the unit-cell parameters varied smoothly, indicating no phase transition. The volume-temperature data were fitted with a polynomial expression for the thermal expansion coefficient (αT=a0+a1T+a2T-2). yieldinga0=2.55(2) × 10–5K–1,al=0 anda2=0 for andalusite, anda0=1.40(4) × 10–5K–1a1=7.1(8) × 10–9K–2anda2=0 for sillimanite. Using the new thermal expansion data determined in the present study and compressional data from the literature, theP-Tphase relations of the kyanite-andalusite-sillimanite system were calculated thermodynamically, with the invariant point located at ∼523°C and 3.93 kbar.
A mutation degree model for the identification of transcriptional regulatory elements
Authors: Zhang Changqing , Wang Jin , Hua Xu , Fang Jinggui , Zhu Huaiqiu , Gao Xiang 
Published: June 2011

Abstract

Background
Current approaches for identifying transcriptional regulatory elements are mainly via the combination of two properties, the evolutionary conservation and the overrepresentation of functional elements in the promoters of co-regulated genes. Despite the development of many motif detection algorithms, the discovery of conserved motifs in a wide range of phylogenetically related promoters is still a challenge, especially for the short motifs embedded in distantly related gene promoters or very closely related promoters, or in the situation that there are not enough orthologous genes available.

Results
A mutation degree model is proposed and a new word counting method is developed for the identification of transcriptional regulatory elements from a set of co-expressed genes. The new method comprises two parts: 1) identifying overrepresented oligo-nucleotides in promoters of co-expressed genes, 2) estimating the conservation of the oligo-nucleotides in promoters of phylogenetically related genes by the mutation degree model. Compared with the performance of other algorithms, our method shows the advantages of low false positive rate and higher specificity, especially the robustness to noisy data. Applying the method to co-expressed gene sets from Arabidopsis, most of known cis-elements were successfully detected. The tool and example are available at http://mcube.nju.edu.cn/jwang/lab/soft/ocw/OCW.html.

Conclusions
The mutation degree model proposed in this paper is adapted to phylogenetic data of different qualities, and to a wide range of evolutionary distances. The new word-counting method based on this model has the advantage of better performance in detecting short sequence of cis-elements from co-expressed genes of eukaryotes and is robust to less complete phylogenetic data.
Production of antibodies with peptide-CpG-DNA-liposome complex without carriers
Authors: Kim Dongbum , Kwon Sanghoon , Rhee Jae Won , Kim Kwang Dong , Kim Young-Eun , Park Cheung-Seog , Choi Myeong Jun , Suh Jun-Gyo , Kim Doo-Sik , Lee Younghee , Kwon Hyung-Joo 
Published: June 2011

AbstractBackgroundThe screening of peptide-based epitopes has been studied extensively for the purpose of developing therapeutic antibodies and prophylactic vaccines that can be potentially useful for treating cancer and infectious diseases such as influenza virus, malaria, hepatitis B, and HIV. To improve the efficacy of antibody production by epitope-based immunization, researchers evaluated liposomes as a means of delivering vaccines; they also formulated adjuvants such as flagella and CpG-DNA to enhance the magnitude of immune responses. Here, we provide a potent method for peptide-based epitope screening and antibody production without conventional carriers.ResultsWe present that a particular form of natural phosphodiester bond CpG-DNA encapsulated in a specific liposome complex (Lipoplex(O)) induces potent immunomodulatory activity in humans as well as in mice. Additionally, Lipoplex(O) enhances the production of IgG2a specific to antigenic protein in mice. Most importantly, immunization of mice with several peptides co-encapsulated with Lipoplex(O) without carriers significantly induces each peptide-specific IgG2a production in a TLR9-dependent manner. A peptide-specific monoclonal antibody produced against hepatocellular carcinoma-associated antigen has functional effects on the cancer cells.ConclusionsOur overall results show that Lipoplex(O) is a potent adjuvant and that complexes of peptide and Lipoplex(O) are extremely useful for B cell epitope screening and antibody production without carriers. Therefore, our strategy may be promptly used for the development of therapeutic antibodies by rapid screening of potent B cell epitopes.
Lactobacillus plantarum (VR1) isolated from an Ayurvedic medicine (Kutajarista) ameliorates in vitro cellular damage caused by Aeromonas veronii
Authors: Kumar Himanshu , Rangrez Ashraf Y , Dayananda Kannayakanahalli M , Atre Ashwini N , Patole Milind S , Shouche Yogesh S 
Published: June 2011

Abstract

Background

Lactobacillus plantarum is considered as a safe and effective probiotic microorganism. Among various sources of isolation, traditionally fermented foods are considered to be rich in Lactobacillus spp., which can be exploited for their probiotic attribute. Antibacterial property of L. plantarum has been demonstrated against various enteric pathogens in both in vitro and in vivo systems. This study was aimed at characterizing L. plantarum isolated from Kutajarista, an ayurvedic fermented biomedicine, and assessing its antagonistic property against a common enteropathogen Aeromonas veronii.

Results
We report the isolation of L. plantarum (VR1) from Kutajarista, and efficacy of its cell free supernatant (CFS) in amelioration of cytotoxicity caused by Aeromonas veronii. On the part of probiotic attributes, VR1 was tolerant to pH 2, 0.3% bile salts and simulated gastric juice. Additionally, VR1 also exhibited adhesive property to human intestinal HT-29 cell line. Furthermore, CFS of VR1 was antibacterial to enteric pathogens like Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli, Aeromonas veronii and clinical isolates of P. aeruginosa and E. coli. Detailed study regarding the effect of VR1 CFS on A. veronii cytotoxicity showed a significant decrease in vacuole formation and detrimental cellular changes in Vero cells. On the other hand, A. veronii CFS caused disruption of tight junction proteins ZO-1 and actin in MDCK cell line, which was prevented by pre-incubation with CFS of VR1.

Conclusions
This is the first study to report isolation of L. plantarum (VR1) from Kutajarista and characterisation for its probiotic attributes. Our study demonstrates the antagonistic property of VR1 to A. veronii and effect of VR1 CFS in reduction of cellular damage caused by A. veronii in both Vero and MDCK cell lines.