bluuebunny/crossref_metadata_2025_split huggingface dataset:
Milk supplementation facilitates appetite control in obese women during weight loss: a randomised, single-blind, placebo-controlled trial
Authors: Gilbert Jo-Anne , Joanisse Denis R. , Chaput Jean-Philippe , Miegueu Pierre , Cianflone Katherine , Alméras Natalie , Tremblay Angelo 
Published: January 2011

Dairy products provide Ca and protein which may facilitate appetite control. Conversely, weight loss is known to increase the motivation to eat. This randomised controlled trial verified the influence of milk supplementation on appetite markers during weight loss. Low Ca consumer women participated in a 6-month energy-restricted programme ( − 2508 kJ/d or − 600 kcal/d) and received either a milk supplementation (1000 mg Ca/d) or an isoenergetic placebo (n 13 and 12, respectively). Fasting appetite sensations were assessed by visual analogue scales. Anthropometric parameters and fasting plasma concentrations of glucose, insulin, leptin, ghrelin and cortisol were measured as well. Both groups showed a significant weight loss (P < 0·0001). In the milk-supplemented group, a time × treatment interaction effect showed that weight loss with milk supplementation induced a smaller increase in desire to eat and hunger (P < 0·05). Unlike the placebo group, the milk-supplemented group showed a lower than predicted decrease in fullness ( − 17·1 v. − 8·8; − 12·7 v. 3·3 mm, P < 0·05, measured v. predicted values, respectively). Even after adjustment for fat mass loss, changes in ghrelin concentration predicted those in desire to eat (r 0·56, P < 0·01), hunger (r 0·45, P < 0·05) and fullness (r − 0·40, P < 0·05). However, the study did not show a between-group difference in the change in ghrelin concentration in response to the intervention. These results show that milk supplementation attenuates the orexigenic effect of body weight loss. Trial registration code: ClinicalTrials.gov NTC00729170.
Derivation of anthropometric cut-off levels to define CVD risk in Sri Lankan adults
Authors: Katulanda P. , Jayawardena M. A. R. , Sheriff M. H. R. , Matthews D. R. 
Published: January 2011

Obesity is associated with increased cardiovascular risk. Anthropometric cut-off values derived for Caucasians may not be applicable to other populations. The main objective of the present study was to derive population-specific anthropometric cut-off values to define high CVD risk for Sri Lankan adults. A nationally representative sample of 4474 non-institutionalised adults aged ≥ 18 years was analysed. Cut-off values to provide optimum sensitivity and specificity were derived using receiver-operating characteristic curve analysis. BMI, waist circumference (WC), waist-to-hip ratio (WHR), blood pressure and overnight fasting venous blood samples were collected to measure glucose, HDL-cholesterol and TAG. An oral glucose tolerance test was also performed. The results suggested that the age-adjusted BMI, WC and WHR were significantly associated with all cardiovascular risk factors (P < 0·001). Cut-off values for BMI, WC and WHR for males were 20·7 kg/m2, 76·5 cm and 0·89, respectively. The respective values for females were 22·0 kg/m2, 76·3 cm and 0·85. The common cut-off value for BMI for males and females was 21·5 kg/m2. Similarly, WC and WHR cut-off values for both males and females were 76·3 cm and 0·87, respectively. The Asian and Caucasian anthropometric cut-off levels showed lower sensitivity and higher false negative percentage compared with newly derived cut-off levels. In conclusion, BMI, WC and WHR were all associated with increased CVD risk. We propose the following anthropometric cut-off points to determine high CVD risk level for Sri Lankan adults: BMI ≥ 21·5 kg/m2, WC ≥ 76 cm and WHR ≥ 0·85 (women) and 0·90 (men).
The antioxidant effects of garlic saponins protect PC12 cells from hypoxia-induced damage
Authors: Luo Hong , Huang Jian , Liao Wei-Gong , Huang Qing-Yuan , Gao Yu-Qi 
Published: January 2011

Hypoxia frequently occurs under several different cellular circumstances. Excess reactive oxygen species that are induced by hypoxia may result in cell injury and dysfunction. Recently, garlic has been found to possess some biological and pharmacological activities. The present study examined the effects of garlic saponins (GSP) on the survival of differentiated PC12 (dPC12) cells and the oxidative–antioxidant system. dPC12 cells were exposed to 2 % O2in order to establish a neuronal insult model. Cell viability was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide reduction assay and lactate dehydrogenase (LDH) release assay. The expression of selected genes (catalase (CAT), p65 and neuron-specific class III β-tubulin) was evaluated by real-time PCR and immunoblot assays. CAT activity, malondialdehyde (MDA) and 8-hydroxy-deoxyguanosine (8-OH-dG) concentrations were also determined. The data showed that hypoxia dramatically damaged dPC12 cells, while treatment with approximately 5 × 10− 2–10 ng/ml GSP improved cell viability, decreased LDH leakage and caused the cells to maintain neuronal-like characteristics in hypoxia. The production of MDA and 8-OH-dG was attenuated by GSP. CAT activity in dPC12 cells pretreated with GSP was higher than that of the hypoxic control. Moreover, GSP up-regulated CAT expression and decreased the total protein expression as well as the nuclear expression of p65 in hypoxic cells. These data indicate that GSP has antioxidant properties that can protect dPC12 cells from hypoxia-induced damage, which may be related to the up-regulation of CAT expression and activity as well as a decrease in the expression and nucleus distribution of p65 through effects on redox-sensitive signalling pathways.
Acute effects of elevated NEFA on vascular function: a comparison of SFA and MUFA
Authors: Newens Katie J. , Thompson Abby K. , Jackson Kim G. , Wright John , Williams Christine M. 
Published: January 2011

There is emerging evidence to show that high levels of NEFA contribute to endothelial dysfunction and impaired insulin sensitivity. However, the impact of NEFA composition remains unclear. A total of ten healthy men consumed test drinks containing 50 g of palm stearin (rich in SFA) or high-oleic sunflower oil (rich in MUFA) on separate occasions; a third day included no fat as a control. The fats were emulsified into chocolate drinks and given as a bolus (approximately 10 g fat) at baseline followed by smaller amounts (approximately 3 g fat) every 30 min throughout the 6 h study day. An intravenous heparin infusion was initiated 2 h after the bolus, which resulted in a three- to fourfold increase in circulating NEFA level from baseline. Mean arterial stiffness as measured by digital volume pulse was higher during the consumption of SFA (P < 0·001) but not MUFA (P = 0·089) compared with the control. Overall insulin and gastric inhibitory peptide response was greater during the consumption of both fats compared with the control (P < 0·001); there was a second insulin peak in response to MUFA unlike SFA. Consumption of SFA resulted in higher levels of soluble intercellular adhesion molecule-1 (sI-CAM) at 330 min than that of MUFA or control (P ≤ 0·048). There was no effect of the test drinks on glucose, total nitrite, plasminogen activator inhibitor-1 or endothelin-1 concentrations. The present study indicates a potential negative impact of elevated NEFA derived from the consumption of SFA on arterial stiffness and sI-CAM levels. More studies are needed to fully investigate the impact of NEFA composition on risk factors for CVD.
Dietary oxidised frying oil causes oxidative damage of pancreatic islets and impairment of insulin secretion, effects associated with vitamin E deficiency
Authors: Chiang Ya-Fan , Shaw Huey-Mei , Yang Mei-Fang , Huang Chih-Yang , Hsieh Cheng-Hsien , Chao Pei-Min 
Published: January 2011

We previously reported that, in rodents, a diet with a high oxidised frying oil (OFO) content leads to glucose intolerance associated with a reduction in insulin secretion. The present study aimed at investigating the impairment of pancreatic islets caused by dietary OFO. C57BL/6J mice were divided into three groups to receive a low-fat basal diet containing 5 g/100 g of fresh soyabean oil (LF group) or a high-fat diet containing 20 g/100 g of either fresh soyabean oil (HF group) or OFO (HO group). After 8 weeks, mice in the HO group showed glucose intolerance and hypoinsulinaemia, and their islets showed impaired glucose-stimulated insulin secretion (P < 0·05; HO groupv.LF and HF groups). Significantly higher oxidative stress and a lower mitochondrial membrane potential were observed in the islets in the HO group compared with the LF and HF groups. Immunoblots showed that the reduction in insulin levels in HO islets was associated with activation of the c-Jun NH2-terminal kinase and a reduction in levels of pancreatic and duodenal homeobox factor-1. In a second study, when dietary OFO-induced tissue vitamin E depletion was prevented by large-dose vitamin E supplementation (500 IU(1·06 mmol all-rac-α-tocopherol acetate)/kg diet; HO+E group), the OFO-mediated reduction in islet size and impairment of glucose tolerance and insulin secretion were significantly attenuated (P < 0·05; HO groupv.HO+E group). We conclude that a high level of dietary OFO ingestion impairs glucose metabolism by causing oxidative damage and compromising insulin secretion in pancreatic islets, and that these effects can be prevented by vitamin E supplementation.
Fluid flow within the damage zone of the Boccheggiano extensional fault (Larderello–Travale geothermal field, central Italy): structures, alteration and implications for hydrothermal mineralization in extensional settings
Authors: ROSSETTI FEDERICO , ALDEGA LUCA , TECCE FRANCESCA , BALSAMO FABRIZIO , BILLI ANDREA , BRILLI MAURO 
Published: January 2011

AbstractThe Neogene extensional province of southern Tuscany in central Italy provides an outstanding example of fossil and active structurally controlled fluid flow and epithermal ore mineralization associated with post-orogenic silicic magmatism. Characterization of the hydrodynamic regime leading to the genesis of the polysulphide deposit (known as Filone di Boccheggiano) hosted within the damage zone of the Boccheggiano Fault is a key target to assess modes of fossil hydrothermal fluid circulation in the region and, more generally, to provide inferences on fault-controlled hydrothermal fluid flow in extensional settings. We provide a detailed description of the fault zone architecture and alteration/mineralization associated with the Boccheggiano ore deposit and report the results of fluid inclusion and stable oxygen isotope studies. This investigation shows that the Boccheggiano ore consists of an adularia/illite-type epithermal deposit and that sulphide ore deposition was controlled by channelling of hydrothermal fluids of dominantly meteoric origin within the highly anisotropic permeability structure of the Boccheggiano Fault. The low permeability structure of the fault core compartmentalized the fluid outflow preventing substantial cross-fault flow, with focused fluid flow occurring at the hangingwall of the fault controlled by fracture permeability. Fluid inclusion characteristics indicate that ore minerals were deposited between 280° and 350°C in the upper levels of the brittle extending crust (lithostatic pressure in the order of 0.1 GPa). Abundant vapour-rich inclusions in ore-stage quartz are consistent with fluid immiscibility and boiling, and quartz ore vein textures suggest that mineralization in the Boccheggiano ore deposit occurred during cyclic fluid flow in a deformation regime regulated by transient and fluctuating fluid pressure conditions. Results from this study (i) predict a strongly anisotropic permeability structure of the fault damage zone during crustal extension, and (ii) indicate the rate of secondary (structural) permeability creation and maintenance by active deformation in the hangingwall of extensional faults as the major factor leading to effective hydraulic transmissivity in extensional terranes. These features intimately link ore-grade mineralization in extensional settings to telescoping of hydrothermal flow along the hangingwall block(s) of major extensional fault zones.
Novel crop science to improve yield and resource use efficiency in water-limited agriculture
Authors: DAVIES W. J. , ZHANG J. , YANG J. , DODD I. C. 
Published: January 2011

SUMMARYGlobally, agriculture accounts for 0·80–0·90 of all freshwater used by humans and, in many crop production systems, this water use is unsustainable. The current paper focuses on the potential exploitation of novel drought stress biology in both crop improvement programmes and via changed crop management practices. The aim is to deliver ‘more crop per drop’. In order to respond to the challenge of feeding a world population of seven billion and growing, it is concluded that an interdisciplinary approach is needed involving new genetic opportunities and plant breeding. It is also shown how crop management can exploit the drought stress physiology of plants to deliver improved water productivity without sacrificing crop yield.
Application of genetics and genomics to aquaculture development: current and future directions
Authors: McANDREW B. , NAPIER J. 
Published: January 2011

SUMMARYGlobal aquaculture production continues to grow rapidly yet a small proportion of the animals and plants being used come from managed breeding and improvement programmes. The biology of aquatic organisms offer many opportunities for rapid genetic gains as new genetic and genomic techniques make the management of improvement programmes feasible in a wider range of species. The current paper describes the application of a wide range of techniques, many unique to aquatic organisms, and their potential to secure aquaculture production in the future.
Statistical control charts as a support tool for the management of livestock production
Authors: MERTENS K. , DECUYPERE E. , DE BAERDEMAEKER J. , DE KETELAERE B. 
Published: January 2011

SUMMARYThe concepts of control charts, an important tool in statistical process control, are commonly used for monitoring industrial production processes. In the context of precision livestock farming, their use has been demonstrated by many, although the statistical properties of livestock process data often do not comply with the basic assumptions of such control charts. The focus of the current review is on the most important aspects, recommendations, pitfalls and opportunities for the development and performance of control charts on livestock process data. An important hurdle to tackle is the statistical characteristics of the raw livestock process data which are mostly violating the control charts’ assumptions. An integrated approach, like synergistic control, appears to be promising in handling this issue. The availability of real-time on-farm validation of proposed systems will be crucial for lifting them from the potential level to direct practical relevance.
Three novel mutations inASIPassociated with black fibre in alpacas (Vicugna pacos)
Authors: FEELEY N. L. , BOTTOMLEY S. , MUNYARD K. A. 
Published: January 2011

SUMMARYThe coding region of the alpaca Agouti signalling protein (ASIP) gene was sequenced. It was determined to be 402 nucleotides long and code for a protein that is 133 amino acids long. Eight mutations were identified in a sample of 15 alpaca, five in the coding region and three in the introns flanking the exons.In silicoanalysis showed that three of the five mutations in the coding sequence, c.325_381del57, c.292C>T and c.353G>A are probable loss-of-function mutations. The three mutations were strongly associated with black fibre colour, with 0·90 of black alpacas in the current study having two copies of one or another of the mutations. However, not all black animals displayed the putative ‘aa’ genotype, and almost half of the non-black animals did display that genotype. Contributing factors such as regulatory region mutations, interactions of ASIP with melanocortin-1 receptor (MC1R) andα-melanocyte stimulating hormone (α-MSH), the effect of dilution genes and subjective phenotype assignment are discussed. These mutations will allow alpaca breeders to select for or against black, but they do not explain all black phenotypes in this species.
On weakly turbulent scaling of wind sea in simulations of fetch-limited growth
Authors: GAGNAIRE-RENOU ELODIE , BENOIT MICHEL , BADULIN SERGEI I. 
Published: January 2011

Extensive numerical simulations of fetch-limited growth of wind-driven waves are analysed within two approaches: a ‘traditional’ wind-speed scaling first proposed by Kitaigorodskii (Bull. Acad. Sci. USSR, Geophys. Ser., Engl. Transl., vol. N1, 1962, p. 105) in the early 1960s and an alternative weakly turbulent scaling developed recently by Badulin et al. (J. Fluid Mech.591, 2007, 339–378). The latter one uses spectral fluxes of wave energy, momentum and action as physical scales of the problem and allows for advanced qualitative and quantitative analysis of wind-wave growth and features of air–sea interaction. In contrast, the traditional approach is shown to be descriptive rather than proactive. Numerical simulations are conducted on the basis of the Hasselmann kinetic equation for deep-water waves in a wide range of wind speeds from 5 to 30 m s −1 and for the ideal case of fetch-limited growth: permanent wind blowing perpendicularly to a straight coastline. Two different wave input functions, Sin, and two methods for calculating the nonlinear transfer term Snl (Gaussian quadrature method, or GQM, a quasi-exact method based on the use of Gaussian quadratures, and the discrete interaction approximation, or DIA) are used in the simulations. Comparison of the corresponding results firstly shows the relevance of the analysis of wind-wave growth in terms of the proposed weakly turbulent scaling, and secondly, allows us to highlight some critical points in the modelling of wind-generated waves. Three stages of wind-wave development corresponding to qualitatively different balance of the source terms, Sin, Sdiss and Snl, are identified: initial growth, growing sea and fully developed sea. Validity of the asymptotic weakly turbulent approach for the stage of growing wind sea is determined by the dominance of nonlinear transfers, which results in a rigid link between spectral fluxes and wave energy. This stage of self-similar growth is investigated in detail and presented as a consequence of three sub-stages of qualitatively different coupling of air flow and growing wind waves. The key self-similarity parameter of the asymptotic theory is estimated to be αss = 0.68 ± 0.1.Further prospects of wind-wave modelling in the context of the presented weakly turbulent scaling are discussed.
Gamma-aminobutyric acid concentrations in benign parotid tumours and unstimulated parotid saliva
Authors: Jezewska E , Scinska A , Kukwa W , Sobolewska A , Turzynska D , Samochowiec J , Bienkowski P 
Published: January 2011

AbstractObjective:Apart from its role as an inhibitory neurotransmitter, γ-aminobutyric acid is also thought to regulate various stages of cell proliferation and differentiation in the brain and periphery. The present study aimed to assess the levels of γ-aminobutyric acid and its biochemical precursor glutamic acid (glutamate) in benign parotid tumours and in unstimulated parotid saliva.Method:Unstimulated parotid saliva was collected bilaterally, using the swab method, in 20 patients with unilateral pleomorphic adenoma or Warthin's tumour. Samples of tumour and adjacent salivary tissue were collected during tumour resection.Results:Concentrations of γ-aminobutyric acid and glutamate, but not aspartate, were significantly higher in the tumour tissue than in the non-tumour tissue. There was no significant difference in salivary concentrations of γ-aminobutyric acid, glutamate or aspartate, comparing the involved and non-involved side.Conclusion:The present results provide preliminary evidence that γ-aminobutyric acid may be involved in the growth of benign parotid tumours.
A case of middle-ear cavernous lymphangioma with facial palsy
Authors: Hirai R , Ikeda M , Kishi H , Nomura Y , Shigihara S 
Published: January 2011

AbstractObjective:Only a few benign tumours of the middle ear have been reported to lead to the development of facial palsy. Here, we describe a patient with middle-ear cavernous lymphangioma and facial palsy.Study design:Single case study.Patient:A 61-year-old man presented with left-sided hearing impairment and incomplete left facial palsy. A tumour was confirmed to be occupying the epi- to mesotympanum and to be joined to the facial nerve. The tumour was removed along with facial nerve tissue, which was resected at its horizontal portion, and the remaining facial nerve was fixed by end-to-end anastomosis. Complete facial paralysis occurred after the operation, but the patient's House–Brackmann grade gradually improved to grade III. Post-operative histopathological examination revealed infiltration of the lymphangioma into the facial nerve tissue, together with mild neural atrophy of the facial nerve.Conclusion:These findings suggested that tumour invasion was the cause of facial palsy in this patient.
Association of age-related macular degeneration with age-related hearing loss
Authors: Bozkurt M K , Ozturk B T , Kerimoglu H , Ersan I , Arbag H , Bozkurt B 
Published: January 2011

AbstractObjective:To assess the association between age-related macular degeneration and age-related hearing loss in Turkish subjects aged 50 years or older.Study design and setting:Prospective, case–control study within a tertiary university hospital.Subjects and methods:Fifty subjects with age-related macular degeneration and 43 healthy subjects underwent ophthalmological and otolaryngological examination. Statistical analyses were conducted for the poorer eye and ear, comparing age-related hearing loss and pure tone average in the macular degeneration group versus controls.Results:Median pure tone average was significantly poorer in the macular degeneration group (35 dBHL) compared with controls (23 dBHL). In the macular degeneration group, hearing loss was significantly greater in dry type (43 dBHL) than wet type (32 dBHL) cases. There was a significant difference between the prevalence of varying degrees of hearing loss in the macular degeneration versus control groups, being respectively: mild, 50 and 35 per cent; moderate, 20 and 5 per cent; and severe, 6 and 0 per cent. There was a weak, but significant correlation between each patient's visual acuity and pure tone average results (rs = −0.37, p < 0.001).Conclusion:Age-related hearing loss is more common in patients with age-related macular degeneration. Such patients should be questioned regarding hearing difficulty, and referred to an otolaryngologist if appropriate.
A new species and new records ofLecanora(Lecanoraceae, Ascomycota) from south–east Asia
Authors: PAPONG Khwanruan , BOONPRAGOB Kansri , LUMBSCH H. Thorsten 
Published: January 2011

AbstractThe new speciesLecanora ulrikiifrom Bhutan and Thailand is described. It belongs toLecanoras. str. and is characterized by relatively large, orange-brown to brown apothecia that are constricted at the base, a clear to inspersed hymenium, an epihymenium of theglabrata-type, and by the presence of atranorin, usnic acid and the isoarthothelin chemosyndrome. Further, three species,L. arthothelinella, L. austrotropicaandL. subimmergens, are reported for the first time from Thailand.