bluuebunny/crossref_metadata_2025_split huggingface dataset:
Receptor tyrosine kinase pathway analysis sheds light on similarities between clear‐cell sarcoma and metastatic melanoma
Authors: Negri Tiziana , Brich Silvia , Conca Elena , Bozzi Fabio , Orsenigo Marta , Stacchiotti Silvia , Alberghini Marco , Mauro Valentina , Gronchi Alessandro , Dusio Giuseppina F. , Pelosi Giuseppe , Picci Piero , Casali Paolo G. , Pierotti Marco A. , Pilotti Silvana 
Published: November 2011

AbstractTo highlight possible similarities and differences in receptor tyrosine kinase (RTK) and downstream signalling activation profiles between clear‐cell sarcomas (CCS) and metastatic melanomas (MM), frozen, and paired‐matched fixed samples of six CCS with EWSR1 rearrangement (EWSR1+), five CCS without EWSR1 rearrangement (EWSR1−), and seven MM were investigated by means of biochemical, immunohistochemical, FISH, molecular analyses, and immunofluorescence confocal microscopy. Fixed samples of a further 10 CCS and 14 MM were investigated by means of sequencing for BRAF, NRAS, and KRAS mutations and FISH analyses for the gain of chromosomes 22 and 8. RTK analysis of all CCS/MM samples showed activation of short‐form (sf) recepteur d'origine nantais (RON) RTK and of PDGFRB, MET, and HER3. Analysis of downstream signaling revealed consistent phosphorylation patterns of PI3K/AKT, RSK, and the mTOR targets S6 and 4EBP1. Analysis of frozen and fixed material from 21 CCS and 21 MM showed the presence of the V600E BRAF mutation in 2/12 EWSR1+ and 3/9 EWSR1− CCS and 9/21 MM and demonstrated a significant (P < 0.001) correlation between the gain of chromosomes 22 and 8 and EWSR1− CCS. Our results show that BRAF mutation can also be present in CCS and support the proposed aberration of chromosomes 22 and 8 as a possibly useful nonrandom hallmark of EWSR1− CCS. Besides, they broaden the spectrum of the similarities of RTK pathway activation between CCS and MM, thus suggesting that new drugs found to be active in melanoma and RON inhibitors could have a role in CCS treatment. © 2011 Wiley Periodicals, Inc.
PTEN genomic deletions that characterize aggressive prostate cancer originate close to segmental duplications
Authors: Yoshimoto Maisa , Ludkovski Olga , DeGrace Dave , Williams Julia L. , Evans Andrew , Sircar Kanishka , Bismar Tarek A. , Nuin Paulo , Squire Jeremy A. 
Published: November 2011

AbstractDeletion of PTEN at 10q23.3 occurs in ∼40% of human prostate cancers and is associated with aggressive metastatic potential, poor prognosis, and androgen‐independence. This high frequency of recurrent PTEN deletions in prostate cancer suggests there may be unusual genomic features close to this locus that facilitate DNA alteration at 10q23.3. To explore possible mechanisms for deletions in the PTEN region, a meta‐analysis of 311 published human genome array datasets was conducted and determined that the minimal prostate cancer‐associated deletion at 10q23.3 corresponds to ∼2.06 MB region flanked by BMPR1A and FAS. On a separate cohort comprising an additional 330 tumors, four‐color fluorescence in situ hybridization analysis using probes for BMPR1A, FAS, cen(10), and PTEN showed that 132 of 330 (40%) tumors had PTEN loss, 50 (15%) of which were homozygous losses (comprising in total 100 deletion events). Breakpoints between PTEN and BMPR1A or FAS were subsequently mapped in 100 homozygous and 82 hemizygous PTEN losses, revealing that 125/182 PTEN microdeletions occurred within the 940 kB interval between BMPR1A and PTEN. Furthermore, this breakpoint interval coincides with a repeat‐rich region of 414 kB containing the SD17 and SD18 segmental duplications, which contain at least 13 homologous inverted repeat sequences. Together, these data suggest that a strong selective growth advantage for loss of PTEN and upregulation of PI3K/AKT, combined with the close proximity of PTEN to a large unstable segment of repeated DNA comprising SD17 and SD18, can lead to recurrent microdeletions of the PTEN gene in prostate cancer. © 2011 Wiley Periodicals, Inc.
Ultrastructural analysis of implant–soft tissue interface on a three dimensional tissue‐engineered oral mucosal model
Authors: Chai Wen L. , Brook Ian M. , Emanuelsson Lena , Palmquist Anders , van Noort Richard , Moharamzadeh Keyvan 
Published: November 2011

AbstractA three dimensional tissue‐engineered human oral mucosal model (3D OMM) used in the investigation of implant–soft tissue interface was recently reported. The aim of this study was to examine the ultrastructural features of soft tissue attachment to various titanium (Ti) implant surfaces based on the 3D OMM. Two techniques, that is, focus ion beam (FIB) and electropolishing techniques were used to prepare specimens for transmission electron microscopic (TEM) analysis of the interface. The 3D OM consisting of both epithelial and connective tissue layers was constructed by co‐culturing human oral keratinocytes and fibroblasts onto an acellular dermis scaffold. Four types of Ti surface topographies were tested: polished, machined (turned), sandblasted, and TiUnite. The specimens were then processed for TEM examination using FIB (Ti remained) and electropolishing (Ti removed) techniques. The FIB sections showed some artifact and lack of details of ultrastructural features. In contrast, the ultrathin sections prepared from the electropolishing technique showed a residual Ti oxide layer, which preserved the details for intact ultrastructural interface analysis. There was evidence of hemidesmosome‐like structures at the interface on the four types of Ti surfaces, which suggests that the tissue‐engineered oral mucosa formed epithelial attachments on the Ti surfaces. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
Theoretical study on the reaction CX3 + SiH(CH3)3 (X = H, F)
Authors: Zhang Hui , Yang Liu , Liu Jing‐Yao , Li Ze‐Sheng 
Published: November 2011

AbstractTheoretical investigations are carried out on the multiple‐channel reactions, CH3 + SiH(CH3)3 → products and CF3 + SiH(CH3)3 → products. The minimum energy paths (MEP) are calculated at the MP2/6‐311 + G(d,p) level, and energetic information is further refined by the MC‐QCISD (single point) method. The rate constants for major reaction channels are calculated by the canonical variational transition state theory (CVT) with small‐curvature tunneling (SCT) correction over the temperature range 200–1500 K. The theoretical rate constants are in good agreement with the available experimental data and are found to be k1a(T) = 1.93 × 10−24T3.15exp(−1214.59/T) and k2a(T) = 1.33 × 10−25T4.13exp(−397.94/T) (in unit of cm3molecule−1s−1). Our calculations indicate that hydrogen abstraction channel from SiH group is the major channel due to the smaller barrier height among five channels considered. © 2011 Wiley Periodicals, Inc. J Comput Chem, 2011
Risk factors of workplace violence at hospitals in Japan
Authors: Fujita Shigeru , Ito Shinya , Seto Kanako , Kitazawa Takefumi , Matsumoto Kunichika , Hasegawa Tomonori 
Published: November 2011

AbstractBACKGROUND:Patients and their relatives exposed to mental stress caused by hospitalization or illness might use violence against healthcare staff and interfere with quality healthcare.OBJECTIVE:The aim of this study was to investigate incidences of workplace violence and the attributes of healthcare staff who are at high risk.DESIGN:A questionnaire‐based, anonymous, and self‐administered cross‐sectional survey.SETTING:Healthcare staff (n = 11,095) of 19 hospitals in Japan.MEASUREMENTS:Incidence rates and adjusted odd ratios of workplace violence were calculated to examine the effect of attributes of healthcare staff to workplace violence by using logistic regression analysis.RESULTS:The response rate for survey completion was 79.1% (8711/11,095). Among the respondents, 36.4% experienced workplace violence by patients or their relatives in the past year; 15.9% experienced physical aggression, 29.8% experienced verbal abuse, and 9.9% experienced sexual harassment. Adjusted odds ratios of physical aggression were significantly high in psychiatric wards, critical care centers/intensive care units (ICU)/cardiac care units (CCU), long‐term care wards, for nurses, nursing aides/care workers, and for longer working hours. Adjusted odds ratios of verbal abuse were significantly high in psychiatric wards, long‐term care wards, outpatient departments, dialysis departments, and for longer years of work experience, and for longer working hours. Adjusted odds ratios of sexual harassment were significantly high in dialysis departments, for nurses, nursing aides/care workers, technicians, therapists and females. The general ward and direct interaction with patients were common risk factors for each type of workplace violence.CONCLUSIONS:The mechanisms and the countermeasures for each type of workplace violence at those high‐risk areas should be investigated. Journal of Hospital Medicine 2012;. © 2011 Society of Hospital Medicine.
Automated multiscale vessel analysis for the quantification of MR angiography of peripheral arteriogenesis
Authors: Jaspers Karolien , Slenter Jos M.G.M. , Leiner Tim , Wagenaar Allard , Post Mark J. , Backes Walter H. 
Published: November 2011

AbstractPurpose:To automatically analyze the time course of collateralization in a rat hindlimb ischemia model based on signal intensity distribution (SID).Materials and Methods:Time‐of‐flight magnetic resonance angiograms (TOF‐MRA) were acquired in eight rats at 2, 7, and 21 days after unilateral femoral artery ligation. Analysis was performed on maximum intensity projections filtered with multiscale vessel enhancement filter. Differences in SID between ligated limb and a reference region were monitored over time and compared to manual collateral artery identification.Results:The differences in SID correlated well with the number of collateral arteries found with manual quantification. The time courses of ultrasmall (diameter ≪0.5 mm) and small (diameter ≈0.5 mm) collateral artery development could be differentiated, revealing that maturation of the collaterals and enlargement of their feeding arteries occurred mainly after the first week postligation.Conclusion:SID analysis performed on axial maximum intensity projections is easy to implement, fast, and objective and provides more insight in the time course of arteriogenesis than manual identification. J. Magn. Reson. Imaging 2012;379‐386. © 2011 Wiley Periodicals, Inc.
In vivo assessment of neurodegeneration in niemann‐pick type C mice by quantitative T2 mapping and diffusion tensor imaging
Authors: Totenhagen John W. , Lope‐Piedrafita Silvia , Borbon Ivan A. , Yoshimaru Eriko S. , Erickson Robert P. , Trouard Theodore P. 
Published: November 2011

AbstractPurpose:To quantitatively and noninvasively assess neurological disease progression in a mouse model of Niemann‐Pick type C (NPC) disease by measuring white matter status with magnetic resonance imaging (MRI) techniques of T2 mapping and diffusion tensor imaging (DTI).Materials and Methods:Quantitative T2 and DTI experiments were performed in vivo in NPC disease model and control mice at three timepoints to quantify differences and changes in white matter with measurements of T2 relaxation and DTI parameters. Histological staining for myelin content was also performed at two timepoints to compare with the MRI findings.Results:The results of the T2 and DTI measurements show significant differences in white matter areas of the brain in the NPC disease model compared to control mice at several timepoints, and were seen to change over time in both groups.Conclusion:The findings of this study suggest that quantitative MRI measurements may be suitable in vivo biomarkers of disease status for future studies of NPC disease models. The changes in white matter measurements between timepoints in both control and NPC disease groups suggest that white matter structures continue to change and develop over time in the NPC model and can be tracked with MRI techniques. J. Magn. Reson. Imaging 2012;35:528‐536. © 2011 Wiley Periodicals, Inc.
Focal cortical lesion detection in multiple sclerosis: 3 tesla DIR versus 7 tesla FLASH‐T2*
Authors: Nielsen A. Scott , Kinkel R. Philip , Tinelli Emanuele , Benner Thomas , Cohen‐Adad Julien , Mainero Caterina 
Published: November 2011

AbstractPurpose:To evaluate the inter‐rater agreement of cortical lesion detection using 7 Tesla (T) FLASH‐T2* and 3T DIR sequences.Materials and Methods:Twenty‐six patients with multiple sclerosis were scanned on a human 7T (Siemens) and 3T MRI (TIM Trio, Siemens) to acquire 3T DIR/MEMPR and 7T FLASH‐T2* sequences. Four independent reviewers scored and categorized cortical lesions in the bilateral precentral gyri (motor strips) as leukocortical, intracortical, or subpial. Inter‐rater agreement was assessed according to lesion category using the kappa statistic. The sensitivity of recent MAGNIMS consensus guidelines for cortical lesion detection using 3T DIR was assessed with 7T FLASH‐T2* as the reference gold standard.Results:Inter‐rater agreement at 7T was excellent compared with 3T (k = 0.97 versus 0.12). FLASH‐T2* at 7T detected subpial lesions while 3T DIR did not. The predicted sensitivity of 3T DIR sequence for cortical lesions in vivo is modest (range of 13.6 to 18.3%).Conclusion:The 7T FLASH‐T2* detects more cortical—particularly subpial—lesions compared with 3T DIR. In the absence of DIR/postmortem data, 7T FLASH‐T2* is a suitable gold‐standard instrument and should be incorporated into future consensus guidelines. J. Magn. Reson. Imaging 2012;537‐542. © 2011 Wiley Periodicals, Inc.
A longitudinal MRI study of muscle atrophy during lower leg immobilization following ankle fracture
Authors: Psatha Maria , Wu Zhiqing , Gammie Fiona M. , Ratkevicius Aivaras , Wackerhage Henning , Lee Jennifer H. , Redpath Thomas W. , Gilbert Fiona J. , Ashcroft George P. , Meakin Judith R. , Aspden Richard M. 
Published: November 2011

AbstractPurpose:To investigate MRI biomarkers of muscle atrophy during cast immobilization of the lower leg.Materials and Methods:Eighteen patients (8 male, 10 female), who had one lower leg immobilized in a cast, underwent 3.0 Tesla (T) MR imaging 5, 8, 15, 29, and 43 days after casting. Measurements were made on both lower legs of total muscle volume. Cross‐sectional area (CSA), fractional water content, and T2 were measured in tibialis anterior (TA), gastrocnemius medialis (GM) and lateralis (GL) and soleus (SOL). Fiber pennation angle was measured in GM.Results:Total muscle volume decreased by 17% (P < 0.001) over the 6 weeks of immobilization. The greatest loss in CSA (mean[SD]) was seen in GM (−23.3(8.7)%), followed by SOL (−19.0(9.8)%), GL (−17.1(6.5)%), and TA (−10.7(5.9)%). Significant reductions of CSA were also detectable in the contra‐lateral leg. T2 increased in all muscles: TA 27.0(2.5) ms to 29.6(2.8) ms (P < 0.001), GM 34.6(2.9) ms to 39.8(5.4) ms (P < 0.001) and SOL 34.4 (2.9) ms to 44.9(5.9) ms (P < 0.001). Small reductions were found in fractional water content. Pennation angle decreased in the cast leg (P < 0.001).Conclusion:Quantitative MR imaging can detect and monitor progressive biochemical and biophysical changes in muscle during immobilization. J. Magn. Reson. Imaging 2012;35:686‐695. © 2011 Wiley Periodicals, Inc.
MR elastography of the in vivo abdominal aorta: A feasibility study for comparing aortic stiffness between hypertensives and normotensives
Authors: Kolipaka Arunark , Woodrum David , Araoz Philip A. , Ehman Richard L. 
Published: November 2011

AbstractPurpose:To demonstrate feasibility of using MR elastography (MRE) to identify hypertensive changes in the abdominal aorta when compared with normotensives based on the stiffness measurements.Materials and Methods:MRE was performed on eight volunteers (four normotensives and four hypertensives) to measure the effective stiffness of the abdominal aorta. MRE wave images are directionally filtered and phase gradient analysis was performed to determine the stiffness of the aorta. Student's t‐test was performed to determine significant difference in stiffness measurements between normotensives and hypertensives.Results:The normotensive group demonstrated a mean abdominal aortic stiffness of 3.7 ± 0.8 kPa, while the controlled‐hypertensive demonstrated a mean abdominal aortic stiffness of 9.3 ± 1.9 kPa. MRE effective stiffness of abdominal aorta in hypertensives was significantly greater than that of normotensives with p = 0.02.Conclusion:Feasibility of in vivo aortic MRE is demonstrated. Hypertensives have significantly higher aortic stiffness assessed through MRE than normotensives. J. Magn. Reson. Imaging 2012;35:582‐586. © 2011 Wiley Periodicals, Inc.
TDP‐43 toxicity is mediated by the unfolded protein response‐unrelated induction of C/EBP homologous protein expression
Authors: Suzuki Hiroaki , Matsuoka Masaaki 
Published: November 2011

AbstractTransactive response DNA‐binding protein‐43 (TDP‐43) neuronal toxicity plays an essential role in the pathogenesis of amyotrophic lateral sclerosis and frontotemporal lobar degeneration with ubiquitin‐positive inclusions. In our previous study, we showed that low‐grade overexpression of TDP‐43, which is thought to mimic the gain‐of‐function of TDP‐43, caused neuronal death, mediated by the upregulation of Bim and the downregulation of Bcl‐xL in vitro. In this study, we show that TDP‐43 overexpression caused the upregulation of C/EBP‐homologous protein (CHOP) and that disruption of the CHOP gene markedly attenuated TDP‐43‐induced cell death. These results indicate that increases in CHOP expression contribute to TDP‐43‐induced cell death. We also show that the TDP‐43‐induced upregulation of CHOP expression is mediated by both the upregulation of the mRNA level of CHOP and the attenuation of thedegradation of CHOP, which is independent on the PERK/eIF2α/ATF4 or other pathway related to the unfolded protein response (UPR) to endoplasmic reticulum stress. This study provides the first example of the CHOP‐mediated cell death that is independent of the UPR. © 2011 Wiley Periodicals, Inc.
Susceptibility of a Vibrio alginolyticus rpoS mutant to environmental stresses and its expression of OMPs
Authors: Shuxian Wang , Jianteng Wei , Tianbao Li 
Published: November 2011

AbstractVibrio alginolyticus, one of the most important opportunistic pathogens, can be detected in human being and marine animals. Like other bacteria, V. alginolyticus is able to adapt to a variety of stressful environmental changes. The alternate sigma factor RpoS, which is a regulator during stationary phase, plays an important role in surviving under these stressful situations in many bacteria. Sequence analysis reveals a 990 bp open reading frame which is predicted to encode a 330‐amino‐acid protein with 68% to 96% overall identity to other reported sequences. To study the function of rpoS, the rpoS gene of V. alginolyticus VIB283 was cloned and an rpoS mutant was constructed by homologous recombination. Comparison of the study result of the wild type and the mutant showed that the mutant was more sensitive to stress conditions such as high osmolarity, oxidative stress, heat shock, and long‐term starvation and that the LD50 of the mutant strain to the zebra fish was about 2.8 times as that of the control strain. In addition, the SDS‐PAGE analysis indicated that the outer membrane proteins (OMPs) existed great differences. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Myofibroblast cells are preferentially expressed early in a rabbit model of joint contracture
Authors: Abdel Matthew P. , Morrey Mark E. , Barlow Jonathan D. , Kreofsky Cole R. , An Kai‐Nan , Steinmann Scott P. , Morrey Bernard F. , Sanchez‐Sotelo Joaquin 
Published: November 2011

AbstractStudies have demonstrated increased myofibroblasts in contractures. However, the timeline is largely unknown. The aim of this study was to determine the number of myofibroblasts in contracting joint capsules at 3‐time points over a 24‐week period. Eighteen rabbits subjected to a surgical procedure designed to elicit a knee joint contracture were divided into three groups of six. Rabbits were sacrificed at each respective time point and myofibroblasts in the joint capsules were quantified using immunohistochemistry. The percent of myofibroblasts was significantly elevated in the operated limbs compared to the control limbs at 2 weeks (20% vs. 7%, respectively; p = 0.014). There was no difference in the percent of myofibroblasts between the operated and control limbs at 8 or 24 weeks (p = 0.96 and 0.07, respectively). The percent of myofibroblasts dropped from 20% at 2 weeks to 3.0% at 8 weeks (p < 0.001). The decrease from 8 to 24 weeks was not significant (p = 0.19). A large proportion of myofibroblasts are present in contracted joints at 2 weeks. By week 8, the proportion of myofibroblasts seem to return to normal. Interventions aimed at affecting the myofibroblast cell in order to prevent fibrosis should be instituted early. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:713–719, 2012
Assessing anxious features in depressed outpatients
Authors: McClintock Shawn M. , Husain Mustafa M. , Bernstein Ira H. , Wisniewski Stephen R. , Trivedi Madhukar H. , Morris David , Alpert Jonathan , Warden Diane , Luther James F. , Kornstein Susan G. , Biggs Melanie M. , Fava Maurizio , Rush A. John 
Published: November 2011

AbstractBoth the 17‐item Hamilton Rating Scale for Depression (HRSD17) and 30‐item Inventory of Depressive Symptomatology – Clinician‐rated (IDS‐C30) contain a subscale that assesses anxious symptoms. We used classical test theory and item response theory methods to assess and compare the psychometric properties of the two anxiety subscales (HRSDANX and IDS‐CANX) in a large sample (N = 3453) of outpatients with non‐psychotic major depressive disorder in the Sequenced Treatment Alternatives to Relieve Depression (STAR*D) study. Approximately 48% of evaluable participants had at least one concurrent anxiety disorder by the self‐report Psychiatric Diagnostic Screening Questionnaire (PDSQ). The HRSDANX and IDS‐CANX were highly correlated (r = 0.75) and both had moderate internal consistency given their limited number of items (HRSDANX Cronbach's alpha = 0.48; IDS‐CANX Cronbach's alpha = 0.58). The optimal threshold for ascribing the presence/absence of anxious features was found at a total score of eight or nine for the HRSDANX and seven or eight for the IDS‐CANX. It would seem beneficial to delete item 17 (loss of insight) from the HRSDANX as it negatively correlated with the scale's total score. Both the HRSDANX and IDS‐CANX subscales have acceptable psychometric properties and can be used to identify anxious features for clinical or research purposes. Copyright © 2011 John Wiley & Sons, Ltd.
Poly(L‐lysine)/microcrystalline cellulose biocomposites for porous scaffolds
Authors: Eldessouki Mohamed , Buschle‐Diller Gisela , Gowayed Yasser 
Published: November 2011

AbstractThe need for tissue engineered scaffolds is growing due to a shortage in organ donation, potential immunoreactions to allotransplants, and high cost associated with transplantation. The main focus of this research is concerned with material selection and processing which are key for a successful design of any tissue engineered structure. This work investigates the possibility of reinforcing a weak polypeptide [poly(L‐lysine)] with a stronger polysaccharide (cellulose) and processing the resulting composite into a porous structure. As the main processing parameters, the effect of pH on the secondary structure of the polypeptide and the effect of the hydrolysis conditions on the properties of commercially available microcrystalline cellulose (MCC) were studied. The significance of the cellulose content as well as the scaffold fabrication conditions on the properties of the composite system was assessed. Overall, PLL/MCC composites showed a lower crystallinity compared to the PLL alone while further hydrolyzed MCC particles (HMCC) showed surface erosion and resulted in a crystallinity increase when incorporated into a composite structure. POLYM. COMPOS., 2011. © 2011 Society of Plastics Engineers