bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous left maxillary and right mandibular reconstructions with a split osteomyocutaneous peroneal artery–based combined flap
Authors: Nguyen Dung H. , Wu Chih‐Wei , Huang Jung‐Ju , Chang Chun‐Shin , Cheng Ming‐Huei 
Published: October 2011

AbstractBackgroundTraditionally, reconstruction of concurrent maxillary and mandibular defects on opposite sides of the facial skeleton often requires use of 2 free osseocutaneous flaps. A new technique of using a differentially split osteomyocutaneous peroneal artery–based combined (OPAC) flap for 1‐stage reconstruction of left maxillary and right mandibular defects is presented.MethodsAn OPAC flap with 1 skin paddle and a cuff of soleus muscle was harvested. The fibula was split; the distal osseous flap was used for right mandibular reconstruction, and the proximal OPAC flap composing of proximal fibula, skin paddle, and soleus muscle was used for the maxillary reconstruction. Bilateral facial vessels were used as recipient vessels.ResultsNo complications were encountered. The patient showed good functional and cosmetic outcomes at 1‐year follow‐up.ConclusionThe split OPAC flap allows for simultaneous reconstruction of bony defects that are spatially far apart without the need for a second free flap. © 2011 Wiley Periodicals, Inc. Head Neck, 2013
Highly selective A1‐adenosine‐agonist (2‐chloro‐N6‐cyclopentyladenosine) and reduction of flap necrosis in adipocutaneous flaps in rats
Authors: Dacho Andreas K. , Lyutenski Stefan , Aust Gabriela , Dietz Andreas 
Published: October 2011

AbstractBackgroundThe 2‐chloro‐N6‐cyclopentyladenosine (CCPA) was proven to be a protective factor in ischemic reperfusion injury in myocardium and to reduce the infarct size in the heart. The purpose of this study was to determine whether flap necrosis could be reduced by intravenous administration of CCPA.MethodsFifty‐six male Wistar rats were divided into 4 experimental groups. An epigastric adipocutaneous flap was raised, and the area of flap necrosis was assessed for all groups on the fifth postoperative day with planimetry software.ResultsThe control group had a significantly lower rate of flap necrosis than the ischemic control group (p < .05). The nonischemic CCPA group had a significantly lower rate of flap necrosis than the nonischemic control group (p < .05). The ischemic CCPA group had a highly significant (p < .0001) rate of lower flap necrosis than the ischemic control group.ConclusionOur data show that reduction of flap necrosis can be achieved both with and without ischemic periods by intravenous administration of CCPA. © 2011 Wiley Periodicals, Inc. Head Neck, 2011
Participative Approach to Strategy Communication: A Case of Small‐ and Medium‐Sized Metal Enterprises with a Review after Seven Years
Authors: Rajala Hanna‐Kaisa , Väyrynen Seppo 
Published: October 2011

AbstractThis article considers design science issues raised by research on a participative approach to strategy communication (PASC) in small‐ and medium‐sized enterprises in the metal industry to create new visions for the future. It discusses the views of applying participative narrative methods in prescriptively driven research for supporting participation and communication. The article concludes by offering an outline of a framework for an innovative approach to method‐assisted research for a PASC. The target year for the focus group (FG) review was arranged to be 2010 because that particular year was set to be the most opportune and interesting after seven years of the first study. A case study showed that narrative scenarios and storytelling are suitable in a PASC to make a strategy illustrative and plausible as well as to construct a mental model of it. The FG review showed, however, that the anticipated development has not occurred in all matters. A new concept, a “mental model of the future company,'' is tentatively proposed as a tool for a PASC. © 2011 Wiley Periodicals, Inc.
Radiolabeled iodohypericin as tumor necrosis avid tracer: diagnostic and therapeutic potential
Authors: Van de Putte Marie , Marysael Thierry , Fonge Humphrey , Roskams Tania , Cona Marlein Miranda , Li Junjie , Bormans Guy , Verbruggen Alfons , Ni Yicheng , de Witte Peter A.M. 
Published: October 2011

AbstractIt is estimated that 30–80% of solid tumor mass represents necrotic tissue that consists out of a significant number of dead and dying cells. The fact that these necrotic zones are restricted to dysplastic and malignant tissue and are rarely present in normal tissue makes necrosis an interesting target both for cancer diagnosis and therapy. In this study, the avidity of hypericin, [123I]iodohypericin and [131I]iodohypericin to tumor necrosis was explored for both diagnosis and therapy of experimental malignancies. The intratumoral distribution in RIF‐1 tumors was investigated by means of fluorescence microscopy (hypericin) and autoradiography ([123I]iodohypericin). Results show high uptake of the tracers in necrosis at 24 hr, lasting for up to 72 hr p.i. Ratios of activity of [123I]iodohypericin in necrotic tissue over viable tumor reached up to 19.63 ± 4.66, correlating with 9.20% ID/g in necrosis. Nude mice bearing RIF‐1 tumors that received three injections of 300 μCi over a 3‐week treatment period showed stabilization in tumor growth for 5 days, as measured by caliper and micro‐positron emission tomography using [18F]fluorodeoxyglucose. Based on these results, we suggest the potentials of radiolabeled hypericin (1) in diagnostic aspects including prognosis or staging assessment of bulky necrotic cancers, monitoring of treatments and therapeutic follow‐up; and (2) in cancer treatment based on tumor necrosis. In conclusion, we showed that hypericin radiolabeled with iodine is a necrosis avid tracer that can be used both as a tumor diagnostic and therapeutic.
Epithelial–mesenchymal transition in breast cancer lines is mediated through PDGF‐D released by tissue‐resident stem cells
Authors: Devarajan Eswaran , Song Yao‐Hua , Krishnappa Srinivasalu , Alt Eckhard 
Published: October 2011

AbstractEpithelial–mesenchymal transition (EMT) generates tumor cells with stem cell properties. The aim of our study was to investigate the effects of adipose tissue‐derived stem cells (ASCs) on EMT of cancer cells and to further investigate the mechanisms involved. We demonstrate that conditioned medium from ASCs induces breast cancer cells (4T1) to express mesenchymal markers such as fibronectin, alpha smooth muscle actin and vimentin. Flow cytometry analyses show that ASC‐conditioned medium promotes the expansion of CD44high/CD24low cancer stem cells. Soft agar assays using T47D, BT474 and MCF‐7 breast cancer cells reveals that ASC conditioned medium promotes the anchorage‐independent growth of cancer cells. These effects were inhibited by a neutralizing antibody against platelet‐derived growth factor‐D (PDGF‐D). Furthermore, PDGF‐D treated breast cancer cells grow faster in a mouse model, and this effect could be neutralized by a PDGF antibody. In conclusion, our data show that tissue‐resident stem cells interact with the cancer microenvironment via PDGF‐D, induce EMT in the cancer cells in a paracrine fashion, thereby increasing the number of cancer stem cells and increase tumor growth in a PDGF dependent manner. Our findings shed new light on mechanisms where local tissue‐resident stem cells are able to promote the growth of breast cancer cells. Possibly this could open up a novel selective therapeutic strategy targeting EMT pathways and the specific communication between tissue‐resident normal stem cell and cancer stem cells, assuming that the blockage of PDGF‐D pathways is critical for tumor growth but would not affect normal tissue homeostasis.
High‐resolution analysis of alterations in medullary thyroid carcinoma genomes
Authors: Flicker Karin , Ulz Peter , Höger Harald , Zeitlhofer Petra , Haas Oskar A. , Behmel Annemarie , Buchinger Wolfgang , Scheuba Christian , Niederle Bruno , Pfragner Roswitha , Speicher Michael R. 
Published: October 2011

AbstractHereditary and sporadic medullary thyroid carcinoma (MTC) are closely associated with RET proto‐oncogene mutations. However, the role of additional changes in the tumor genomes remains unclear. Our objective was the identification of chromosomal regions involved in MTC tumorigenesis and to assess their significance by using MTC‐derived cell lines. We used array‐CGH (comparative genomic hybridization) to map chromosomal imbalances in 52 primary tumors and ten metastases. Eleven tumors (11/52, 21%) were hereditary and 41 (41/52, 79%) were sporadic. Among the latter, 15 tumors (15/41, 37%) harbored RET mutations. Furthermore, we characterized five MTC cell lines in detail and evaluated the tumorigenicity by severe combined immunodeficiency (SCID)‐mouse experiments. Most MTCs had only few copy number changes, and losses of chromosomes 1p, 4q, 19p and 22q were observed most frequently. The number of chromosomal aberrations increased in metastases. Twenty‐three percent (12/52) of the primary tumors did not even show any chromosomal gains and losses. We injected three cell lines (two of these were without chromosomal changes and pathogenic RET mutations) into immune deficient SCID mice, and in each case, we observed rapid tumor growth at the injection sites. Our data suggest that MTCs—in contrast to most other tumor entities—do not acquire a multitude of genomic imbalances. SCID mouse experiments performed with chromosomally normal cell lines and without RET mutations suggest that presently unknown submicroscopic genomic changes are sufficient in MTC tumorigenesis.
Polyvinylidene fluoride for proliferation and preservation of bovine corneal endothelial cells by enhancing type IV collagen production and deposition
Authors: Wang Tsung‐Jen , Wang I‐Jong , Chen Yi‐Hsin , Lu Jui‐Nan , Young Tai‐Horng 
Published: October 2011

AbstractIn this study, biomaterials with different hydrophobic properties including polyvinyl alcohol (PVA), poly(ethylene‐co‐vinyl alcohol) (EVAL), tissue culture polystyrene (TCPS), and polyvinylidene fluoride (PVDF) were examined in the bovine corneal endothelial cells (BCECs) culture system to elucidate their possible impact on clinical demand and scientific interest. It was found that BCECs were inhibited to attach onto the PVA surface. Conversely, relatively more hydrophobic biomaterials EVAL, TCPS, and PVDF successfully initiate BCEC adhesion. Compared to EVAL, cultured BCECs on TCPS and PVDF exhibited higher viability. Furthermore, fibroblastic transformation on EVAL and TCPS was observed at day 17, but BCECs maintained typical hexagonal shape on the PVDF surface at day 21. This phenomenon can be rescued by previously coating type IV collagen on TCPS but not on EVAL. In addition, when BCECs were cultured on PVDF, the expressions of gap junction connexin‐43, differentiation marker N‐cadherin, and tight junction ZO‐1 were well‐developed, resembling the physiological phenotypes. After examining the type IV collagen expression by Western blot analysis and protein absorption test, a possible explanation for the better proliferation and preservation of BCECs on the PVDF substrate is that PVDF is a bioactive substratum which enables BCECs to synthesize and reserve more extracellular matrix type IV collagen, paving an important way to provide a more preferential environment for BCEC cultures. Accordingly, promoting CEC growth effects after cell‐biomaterial association may be applied to the tissue engineering of corneal endothelium. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
Adjunct therapeutic plasma exchange for anti‐N‐methyl‐D‐aspartate receptor antibody encephalitis: A case report and review of literature
Authors: Kamran Mirza M. , Pogoriler Jennifer , Paral Kristen , Ananthanarayanan Vijayalakshmi , Mandal Saptarshi , Mazin Abdul , Baron Beverly , Richa Elie 
Published: October 2011

AbstractEncephalitis associated with autoantibodies directed against the N‐methyl‐D‐aspartate receptor (NMDAR) is usually a paraneoplastic syndrome that presents in young females with ovarian teratomas. We report a case of a previously healthy 14‐year‐old girl with sudden‐onset paranoia, hallucinations, hyperactivity, increased speech, decreased sleep, seizures, and violent behavior deteriorating to catatonia. Her cerebrospinal fluid tested positive for anti‐NMDAR antibodies. She was treated with five sessions of therapeutic plasma exchange (TPE) after having failed therapy with antibiotics, intravenous steroids, intravenous immunoglobulin (IVIG), one dose of rituximab, and seven sessions of electroconvulsive therapy (ECT). The American Society for Apheresis assigns a Category III (Grade 2C) recommendation for TPE in paraneoplastic neurologic syndromes; however, apheresis specifically for anti‐NMDAR encephalitis has not been well studied. Literature review revealed two case reports describing outstanding improvement in patients with anti‐NMDAR encephalitis following TPE. We report no improvement in our patient's symptoms after plasma exchange and discuss possible reasons for why it failed along with review of the literature. J. Clin. Apheresis, 2011. © 2011 Wiley Periodicals, Inc.
Impact of heart failure on hip fracture outcomes: A population‐based study
Authors: Cullen Michael W. , Gullerud Rachel E. , Larson Dirk R. , Melton L. Joseph , Huddleston Jeanne M. 
Published: October 2011

AbstractBACKGROUND:Hip fracture and heart failure are becoming more prevalent conditions in hospitalized patients. Despite differences in postoperative outcomes from other intermediate risk procedures, guidelines classify hip fracture repair as an intermediate risk operation.OBJECTIVE:This population‐based study sought to examine the prevalence and incidence of heart failure in hip fracture patients.DESIGN, SETTING, AND PATIENTS:We conducted a population‐based historical cohort study of 1116 Olmsted County, MN residents undergoing 1212 hip surgeries from 1988 through 2002. Data were obtained through medical record review. Heart failure was defined by Framingham criteria.RESULTS:The prevalence of preoperative heart failure in our study population was 27% (327 of 1212 cases). Those with preoperative heart failure demonstrated longer lengths of stay, were more often discharged to a skilled facility, and had higher inpatient mortality rates. Rates of postoperative heart failure were 6.7% at seven days and 21.3% at one year. Postoperative heart failure was more common among those with preoperative heart failure (HR 3.0), and those with preoperative heart failure demonstrated higher postoperative mortality rates. Men had a higher risk of postoperative mortality compared to women. Overall survival was lowest among those with both preoperative and postoperative heart failure.CONCLUSIONS:Heart failure represents a common and serious perioperative condition in hip fracture patients. Hip fracture patients with and without heart failure carry higher postoperative risk than guidelines may suggest. Future work must focus on the perioperative management of hip fracture patients with and without heart failure to mitigate postoperative morbidity. Journal of Hospital Medicine 2011; © 2011 Society of Hospital Medicine.
Calcium signaling in response to fluid flow by chondrocytes in 3D alginate culture
Authors: Degala Satish , Williams Rebecca , Zipfel Warren , Bonassar Lawrence J. 
Published: October 2011

AbstractQuantifying the effects of mechanical loading on the metabolic response of chondrocytes is difficult due to complicated structure of cartilage ECM and the coupled nature of the mechanical stimuli presented to the cells. In this study we describe the effects of fluid flow, particularly hydrostatic pressure and wall shear stress, on the Ca2+ signaling response of bovine articular chondrocytes in 3D culture. Using well‐established alginate hydrogel system to maintain spherical chondrocyte morphology, we altered solid volume fraction to change scaffold mechanics. Fluid velocities in the bulk of the scaffolds were directly measured via an optical technique and scaffold permeability and aggregate modulus was characterized to quantify the mechanical stimuli presented to cells. Ca2+ signaling response to direct perfusion of chondrocyte‐seeded scaffolds increased monotonically with flow rate and was found more directly dependent on fluid velocity rather than shear stress or hydrostatic pressure. Chondrocytes in alginate scaffolds responded to fluid flow at velocities and shear stresses 2–3 orders of magnitude lower than seen in previous monolayer studies. Our data suggest that flow‐induced Ca2+ signaling response of chondrocytes in alginate culture may be due to mechanical signaling pathways, which is influenced by the 3D nature of cell shape. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:793–799, 2012
Geochemistry of Quaternary sediments of the Jeongokri archaeological site, Korea: implications for provenance and palaeoenvironments during the Late Pleistocene
Authors: Kim Jin Cheul , Lee Yong Il , Lim Hyoun Soo , Yi Seonbok 
Published: October 2011

AbstractThe geochemical characteristics of an approximately 6‐m‐long sediment core collected from the Jeongokri archaeological site (Hantan River, Korea) were examined to determine the provenance of the sediments and to reconstruct the palaeoenvironment of the study area during the Late Pleistocene (200–127 ka). The core sediments were subdivided into two parts based on grain size: an upper (0–380 cm depth) and lower part (>380 cm depth). The lower part was deposited primarily by fluvial processes during an interglacial period [marine isotope stage (MIS) 7] with warm and humid conditions. Conversely, the upper part was mainly deposited by aeolian processes during a cold and dry glacial period (MIS 6). Geochemical characteristics, represented by major, trace and rare earth element (REE) compositions, and textures of the Jeongokri core sediments show distinct differences between the upper and lower parts. The mineralogy and major, trace and REE compositions indicate that the influence of felsic source rocks (e.g. granite) was dominant in the lower sediments, which were derived from the Hantan River. In contrast, increased quartz content and the very fine and homogeneous grain size in the upper sediments indicate an aeolian origin. REE ratios and distribution patterns, and geochemical signatures indicate that these sediments were derived predominantly from Chinese loess deposits and partially from local sediments from the Korean Peninsula; the possible source area of the upper sediments is the northern part of the Yellow Sea basin. The Yellow Sea basin, having mixed geochemical signatures between Chinese loess and Korean‐derived sediments, was exposed because of low sea levels during MIS 6. The exposed Yellow Sea basin was located in the path of winter‐monsoon winds, which may have carried a great deal of airborne sediments from the basin to the Jeongokri area. Copyright © 2011 John Wiley & Sons, Ltd.
Metabolic profiling of β‐cryptoxanthin and its fatty acid esters by supercritical fluid chromatography coupled with triple quadrupole mass spectrometry
Authors: Wada Yusuke , Matsubara Atsuki , Uchikata Takato , Iwasaki Yugo , Morimoto Satoshi , Kan Katsuta , Okura Tetsuya , Fukusaki Eiichiro , Bamba Takeshi 
Published: October 2011

AbstractIn this study, a high‐throughput and high‐sensitivity profiling system for β‐cryptoxanthin (βCX) and β‐cryptoxanthin fatty acid ester (βCXFA) was constructed by supercritical fluid chromatography (SFC) coupled with triple quadrupole mass spectrometry (QqQMS). βCX and nine βCXFAs were successfully separated within 20 min using a column packed with octadecylsilyl‐bonded silica particles. The limit of detection was 540 fmol for the free form and 32–130 fmol for the esterified forms. These results demonstrate that both the throughput and the sensitivity of this SFC‐QqQMS system are considerably higher than those of conventional methods. When this system was applied for the analysis of Citrus unshiu, βCX and five βCXFAs were directly detected with much simpler sample pre‐preparation. The analysis of other citrus fruits indicated that the βCXFA profiles varied with their breed variety. Furthermore, gas chromatography‐mass spectrometry was used to analyze total fatty acid profiles in C. unshiu, and the results revealed that the profiles of fatty acids located in βCXFA were distinct. This is the first report on the analysis of βCX and its fatty acid derivatives by SFC‐QqQMS. The profiling system developed in this study will be a powerful tool for investigating xanthophyll fatty acid esters.
The comparison of two column classification systems during the chromatographic analysis of steroids
Authors: Plenis Alina , Balakowska Edyta , Bączek Tomasz 
Published: October 2011

AbstractNowadays, due to the availability of hundreds of brands of reversed‐phase liquid chromatographic columns, the selection of suitable columns can be difficult. Therefore, a good characterization and classification system is very important. Among published papers, the classification system based on quantitative structure–retention relationships and a method developed at the Katholieke Universiteit Leuven also exist. In quantitative structure–retention relationships, retention is evaluated in terms of the chemical structure of the analytes and the physicochemical properties of both the stationary and mobile phase. The second system allows to rank columns due to the values of four parameters and the calculation of specific FKUL‐values for a reference column and to be compared with others. In this paper, the classification systems based both on quantitative structure–retention relationships and the FKUL‐values using principal components analysis were compared. Moreover, the proposed column ranking systems have been checked in clinical practice case considering liquid chromatography determination of six steroid hormones in urine samples. Despite that the matching of both methods is not exactly the same, both classification systems provide simple, reliable and comparable results.
Influence of Swelling on the Shear Mechanical Properties of Monodomain Side‐Chain Liquid‐Crystal Elastomers: Gaussian Versus Non‐Gaussian Elasticity
Authors: Rogez Daniel , Brömmel Felicitas , Finkelmann Heino , Martinoty Philippe 
Published: October 2011

AbstractThe shear mechanical properties of nematic side‐chain liquid‐crystal elastomers swollen with a nematic solvent are studied. The results show that the elasticity of the network is Gaussian for elastomers oriented before crosslinking and non‐Gaussian for the usual twice‐crosslinked elastomers oriented by stretching the network formed after the first crosslinking step. For systems oriented before crosslinking, a crossover between Gaussian and non‐ Gaussian behavior is observed at a particular weight concentration of solvent. In contrast to the current belief, the neoclassical model based on Gaussian rubber elasticity cannot provide a correct description of the twice‐crosslinked systems.
An approximative approach for construction of the Glauber dynamics in continuum
Authors: Finkelshtein Dmitri , Kondratiev Yuri , Kutoviy Oleksandr , Zhizhina Elena 
Published: October 2011

AbstractWe develop a new approach for the construction of the Glauber dynamics in continuum. Existence of the corresponding strongly continuous contraction semigroup in a proper Banach space is shown. Additionally we present the finite‐ and infinite‐volume approximations of the semigroup by families of bounded linear operators.