bluuebunny/crossref_metadata_2025_split huggingface dataset:
Habitat use of barbel (Barbus barbus) in a restored urban Danube tributary
Authors: Popp Stephanie , Pinter Kurt , Schwayer Christoph , Hayes Daniel S. 
Published: April 2024

Tributaries play a vital role in fish spawning and recruitment, significantly influencing mainstem river fish populations. However, in the Anthropocene era, tributaries within river networks suffered ecological degradation due to fragmentation and channelization. This has led to reduced distribution ranges and declining populations of various riverine species, underscoring the urgency of conservation and rehabilitation efforts. Our study explores the potential for reintroducing fish from the mainstem Danube River into the Wien River, an urban tributary that has undergone partial rehabilitation. We assessed habitat use and movement patterns of 20 adult barbel (Barbus barbus), a species classified as ‘near threatened’ in Austria, collected from the Danube River during the spawning season. These barbel were tagged with radio telemetry tags and relocated into the upper reaches of the Wien River, surmounting several artificial barriers in the lower sections. Although spawning activity was not observed among the barbel, possibly due to prevailing water temperatures, our data suggest that the Wien River could function as a viable temporary habitat. Barbel were notably inclined to inhabit deeper pool and run habitats. Their daily movements and home ranges were relatively limited, ranging from 0.0 to 1.1 km and 100 m to 2.9 km, respectively. One contributing factor to restricted movement was the presence of beaver and knotweed dams, which created temporary migration obstacles. On average, the barbel remained within the system for more than a month. Most of these fish migrated downstream at the onset of a significant high-flow event. Our assessment suggests that while the Wien River may not serve as a permanent habitat, it could function as a temporary habitat for migratory fish. However, to ensure accurate assessments of the restored Wien River as a spawning habitat, it is imperative to reevaluate the findings under stable spawning conditions and gather comprehensive data on relevant abiotic factors. This study advocates for the restoration of longitudinal connectivity between tributaries and mainstem rivers as a means to counteract biodiversity loss in Anthropocene river ecosystems.
Myocardial B cells have specific gene expression and predicted interactions in dilated cardiomyopathy and arrhythmogenic right ventricular cardiomyopathy
Authors: Bermea Kevin C. , Duque Carolina , Cohen Charles D. , Bhalodia Aashik , Rousseau Sylvie , Lovell Jana , Zita Marcelle Dina , Mugnier Monica R. , Adamo Luigi 
Published: April 2024

IntroductionGrowing evidence from animal models indicates that the myocardium hosts a population of B cells that play a role in the development of cardiomyopathy. However, there is minimal data on human myocardial B cells in the context of cardiomyopathy.MethodsWe integrated single-cell and single-nuclei datasets from 45 healthy human hearts, 70 hearts with dilated cardiomyopathy (DCM), and 8 hearts with arrhythmogenic right ventricular cardiomyopathy (ARVC). Interactions between B cells and other cell types were investigated using the CellChat Package. Differential gene expression analysis comparing B cells across conditions was performed using DESeq2. Pathway analysis was performed using Ingenuity, KEGG, and GO pathways analysis.ResultsWe identified 1,100 B cells, including naive B cells and plasma cells. Cells showed an extensive network of interactions within the healthy myocardium that included outgoing signaling to macrophages, T cells, endothelial cells, and pericytes, and incoming signaling from endothelial cells, pericytes, and fibroblasts. This niche relied on ECM-receptor, contact, and paracrine interactions; and changed significantly in the context of cardiomyopathy, displaying disease-specific features. Differential gene expression analysis showed that in the context of DCM both naive and plasma B cells upregulated several pathways related to immune activation, including upregulation of oxidative phosphorylation, upregulation of leukocyte extravasation, and, in naive B cells, antigen presentation.DiscussionThe human myocardium contains naive B cells and plasma cells, integrated into a diverse and dynamic niche that has distinctive features in healthy, DCM, and ARVC. Naive myocardial-associated B cells likely contribute to the pathogenesis of human DCM.
Prognostic implications of STK11 with different mutation status and its relationship with tumor-infiltrating immune cells in non-small cell lung cancer
Authors: Zheng Jianqing , Deng Yujie , Huang Bifen , Chen Xiaohui 
Published: April 2024

BackgroundMutations in STK11 (STK11Mut) gene may present a negative impact on survival in Non-small Cell Lung Cancer (NSCLC) patients, however, its relationship with immune related genes remains unclear. This study is to unveil whether overexpressed- and mutated-STK11 impact survival in NSCLC and to explore whether immune related genes (IRGs) are involved in STK11 mutations.Methods188 NSCLC patients with intact formalin-fixed paraffin-embedded (FFPE) tissue available for detecting STK11 protein expression were included in the analysis. After immunohistochemical detection of STK11 protein, patients were divided into high STK11 expression group (STK11High) and low STK11 expression group (STK11Low), and then Kaplan-Meier survival analysis and COX proportional hazards model were used to compare the overall survival (OS) and progression-free survival (PFS) of the two groups of patients. In addition, the mutation data from the TCGA database was used to categorize the NSCLC population, namely STK11 Mutated (STK11Mut) and wild-type (STK11Wt) subgroups. The difference in OS between STK11Mut and STK11Wt was compared. Finally, bioinformatics analysis was used to compare the differences in IRGs expression between STK11Mut and STK11Wt populations.ResultsThe median follow-up time was 51.0 months (range 3.0 - 120.0 months) for real-life cohort. At the end of follow-up, 64.36% (121/188) of patients experienced recurrence or metastasis. 64.89% (122/188) of patients ended up in cancer-related death. High expression of STK11 was a significant protective factor for NSCLC patients, both in terms of PFS [HR=0.42, 95% CI= (0.29-0.61), P&lt;0.001] and OS [HR=0.36, 95% CI= (0.25, 0.53), P&lt;0.001], which was consistent with the finding in TCGA cohorts [HR=0.76, 95%CI= (0.65, 0.88), P&lt;0.001 HR=0.76, 95%CI= (0.65, 0.88), P&lt;0.001]. In TCGA cohort, STK11 mutation was a significant risk factor for NSCLC in both lung squamous cell carcinoma (LUSC) and lung adenocarcinoma (LUAD) histology in terms of OS [HR=6.81, 95%CI= (2.16, 21.53), P&lt;0.001; HR=1.50, 95%CI= (1.00, 2.26), P=0.051, respectively]. Furthermore, 7 IRGs, namely CALCA, BMP6, S100P, THPO, CGA, PCSK1 and MUC5AC, were found significantly overexpressed in STK11-mutated NSCLC in both LUSC and LUAD histology.ConclusionsLow STK11 expression at protein level and presence of STK11 mutation were associated with poor prognosis in NSCLC, and mutated STK11 might probably alter the expression IRGs profiling.
The meta-learning method for the ensemble model based on situational meta-task
Authors: Zhang Zhengchao , Zhou Lianke , Wu Yuyang , Wang Nianbin 
Published: April 2024

IntroductionThe meta-learning methods have been widely used to solve the problem of few-shot learning. Generally, meta-learners are trained on a variety of tasks and then generalized to novel tasks.MethodsHowever, existing meta-learning methods do not consider the relationship between meta-tasks and novel tasks during the meta-training period, so that initial models of the meta-learner provide less useful meta-knowledge for the novel tasks. This leads to a weak generalization ability on novel tasks. Meanwhile, different initial models contain different meta-knowledge, which leads to certain differences in the learning effect of novel tasks during the meta-testing period. Therefore, this article puts forward a meta-optimization method based on situational meta-task construction and cooperation of multiple initial models. First, during the meta-training period, a method of constructing situational meta-task is proposed, and the selected candidate task sets provide more effective meta-knowledge for novel tasks. Then, during the meta-testing period, an ensemble model method based on meta-optimization is proposed to minimize the loss of inter-model cooperation in prediction, so that multiple models cooperation can realize the learning of novel tasks.ResultsThe above-mentioned methods are applied to popular few-shot character datasets and image recognition datasets. Furthermore, the experiment results indicate that the proposed method achieves good effects in few-shot classification tasks.DiscussionIn future work, we will extend our methods to provide more generalized and useful meta-knowledge to the model during the meta-training period when the novel few-shot tasks are completely invisible.
Transcranial photobiomodulation in children aged 2–6 years: a randomized sham-controlled clinical trial assessing safety, efficacy, and impact on autism spectrum disorder symptoms and brain electrophysiology
Authors: Fradkin Yuliy , De Taboada Luis , Naeser Margaret , Saltmarche Anita , Snyder William , Steingold Eugenia 
Published: April 2024

BackgroundSmall pilot studies have suggested that transcranial photobiomodulation (tPBM) could help reduce symptoms of neurological conditions, such as depression, traumatic brain injury, and autism spectrum disorder (ASD).ObjectiveTo examine the impact of tPBM on the symptoms of ASD in children aged two to six years.MethodWe conducted a randomized, sham-controlled clinical trial involving thirty children aged two to six years with a prior diagnosis of ASD. We delivered pulses of near-infrared light (40 Hz, 850 nm) noninvasively to selected brain areas twice a week for eight weeks, using an investigational medical device designed for this purpose (Cognilum™, JelikaLite Corp., New York, United States). We used the Childhood Autism Rating Scale (CARS, 2nd Edition) to assess and compare the ASD symptoms of participants before and after the treatment course. We collected electroencephalogram (EEG) data during each session from those participants who tolerated wearing the EEG cap.ResultsThe difference in the change in CARS scores between the two groups was 7.23 (95% CI 2.357 to 12.107, p = 0.011). Seventeen of the thirty participants completed at least two EEGs and time-dependent trends were detected. In addition, an interaction between Active versus Sham and Scaled Time was observed in delta power (Coefficient = 7.521, 95% CI -0.517 to 15.559, p = 0.07) and theta power (Coefficient = −8.287, 95% CI -17.199 to 0.626, p = 0.07), indicating a potential trend towards a greater reduction in delta power and an increase in theta power over time with treatment in the Active group, compared to the Sham group. Furthermore, there was a significant difference in the condition (Treatment vs. Sham) in the power of theta waves (net_theta) (Coefficient = 9.547, 95% CI 0.027 to 19.067, p = 0.049). No moderate or severe side effects or adverse effects were reported or observed during the trial.ConclusionThese results indicate that tPBM may be a safe and effective treatment for ASD and should be studied in more depth in larger studies.Clinical trial registration: https://clinicaltrials.gov/ct2/show/NCT04660552, identifier NCT04660552.
Neutralization of Interleukin 1-beta is associated with preservation of thalamic capillaries after experimental traumatic brain injury
Authors: Özen Ilknur , Clausen Fredrik , Flygt Johanna , Marklund Niklas , Paul Gesine 
Published: April 2024

IntroductionTraumatic brain injury to thalamo-cortical pathways is associated with posttraumatic morbidity. Diffuse mechanical forces to white matter tracts and deep grey matter regions induce an inflammatory response and vascular damage resulting in progressive neurodegeneration. Pro-inflammatory cytokines, including interleukin-1β (IL-1β), may contribute to the link between inflammation and the injured capillary network after TBI. This study investigates whether IL-1β is a key contributor to capillary alterations and changes in pericyte coverage in the thalamus and cortex after TBI.MethodsAnimals were subjected to central fluid percussion injury (cFPI), a model of TBI causing widespread axonal and vascular pathology, or sham injury and randomized to receive a neutralizing anti-IL-1β or a control, anti-cyclosporin A antibody, at 30 min post-injury. Capillary length and pericyte coverage of cortex and thalamus were analyzed by immunohistochemistry at 2- and 7-days post-injury.Results and ConclusionOur results show that early post-injury attenuation of IL-1β dependent inflammatory signaling prevents capillary damage by increasing pericyte coverage in the thalamus.
Development and validation of a diagnostic model for predicting cervical lymph node metastasis in laryngeal and hypopharyngeal carcinoma
Authors: Wu Xingdong , Xie Yuhua , Zeng Wanting , Wu Xiaoping , Chen Jichuan , Li Genping 
Published: April 2024

ObjectivesThe lymph node status is crucial for guiding the surgical approach for patients with laryngeal and hypopharyngeal carcinoma (LHC). Nonetheless, occult lymph node metastasis presents challenges to assessment and treatment planning. This study seeks to develop and validate a diagnostic model for evaluating cervical lymph node status in LHC patients.Materials and methodsThis study retrospectively analyzed a total of 285 LHC patients who were treated at the Department of Otolaryngology Head and Neck Surgery, Daping Hospital, Army Medical University, from January 2015 to December 2020. Univariate and multivariate logistic regression analyses were employed to construct the predictive model. Discrimination and calibration were used to assess the predictive performance of the model. Decision curve analysis (DCA) was performed to evaluate the clinical utility of the model, and validation was conducted using 10-fold cross-validation, Leave-One-Out Cross Validation, and bootstrap methods.ResultsThis study identified significant predictors of lymph node metastasis in LHC. A diagnostic predictive model was developed and visualized using a nomogram. The model demonstrated excellent discrimination, with a C-index of 0.887 (95% CI: 0.835-0.933). DCA analysis indicated its practical applicability, and multiple validation methods confirmed its fitting and generalization ability.ConclusionThis study successfully established and validated a diagnostic predictive model for cervical lymph node metastasis in LHC. The visualized nomogram provides a convenient tool for personalized prediction of cervical lymph node status in patients, particularly in the context of occult cervical lymph node metastasis, offering valuable guidance for clinical treatment decisions.
The histological and molecular characteristics of early-onset colorectal cancer: a systematic review and meta-analysis
Authors: Lawler Thomas , Parlato Lisa , Warren Andersen Shaneda 
Published: April 2024

BackgroundEarly-onset colorectal cancer (CRC), defined as diagnosis before age 50, has increased in recent decades. Although more often diagnosed at advanced stage, associations with other histological and molecular markers that impact prognosis and treatment remain to be clarified. We conducted a systematic review and meta-analysis concerning the prevalence of prognostic and predictive tumor markers for early- vs. late-onset CRC, including oncogene mutations, microsatellite instability (MSI), and emerging markers including immune cells and the consensus molecular subtypes.MethodsWe systematically searched PubMed for original research articles published between April 2013–January 2024. Included studies compared the prevalence of tumor markers in early- vs. late-onset CRC. A meta-analysis was completed and summary odds ratios (ORs) with 95% confidence intervals (CIs) were obtained from a random effects model via inverse variance weighting. A sensitivity analysis was completed to restrict the meta-analysis to studies that excluded individuals with Lynch syndrome, a hereditary condition that influences the distribution of tumor markers for early-onset CRC.ResultsIn total, 149 articles were identified. Tumors from early-onset CRC are less likely to include mutations in KRAS (OR, 95% CI: 0.91, 0.85-0.98), BRAF (0.63, 0.51-0.78), APC (0.70, 0.58-0.84), and NRAS (0.88, 0.78-1.00) but more likely to include mutations in PTEN (1.68, 1.04-2.73) and TP53 (1.34, 1.24-1.45). After limiting to studies that excluded Lynch syndrome, the associations between early-onset CRC and BRAF (0.77, 0.64-0.92) and APC mutation (0.81, 0.67-0.97) were attenuated, while an inverse association with PIK3CA mutation was also observed (0.88, 0.78-0.99). Early-onset tumors are less likely to develop along the CpG Island Methylator Phenotype pathway (0.24, 0.10-0.57), but more likely to possess adverse histological features including high tumor grade (1.20, 1.15-1.25), and mucinous (1.22, 1.16-1.27) or signet ring histology (2.32, 2.08-2.57). A positive association with MSI status (1.31, 1.11-1.56) was also identified. Associations with immune markers and the consensus molecular subtypes are inconsistent.DiscussionA lower prevalence of mutations in KRAS and BRAF is consistent with extended survival and superior response to targeted therapies for metastatic disease. Conversely, early-onset CRC is associated with aggressive histological subtypes and TP53 and PTEN mutations, which may serve as therapeutic targets.
Transcatheter arterial perfusion chemotherapy combined with lipiodol chemoembolization for advanced colorectal cancer complicated by obstruction
Authors: Ding Xiaolong , Ma Yaozhen , Yin Meipan , Liu Tao , Jin Shuiling , Li Chunxia , Li Xiaobing , Zhang Chenchen , Zhou Gang , Wu Gang 
Published: April 2024

BackgroundObstruction is a common complication of advanced colorectal cancer. This study was aimed at investigating the safety, efficacy, and feasibility of transcatheter arterial perfusion chemotherapy combined with lipiodol chemoembolization for treating advanced colorectal cancer complicated by obstruction.Patients and methodsThis retrospective analysis was conducted using clinical data of patients with advanced colorectal cancer who received arterial infusion chemotherapy combined with lipiodol chemoembolization treatment at our center. Treatment efficacy was evaluated in terms of obstruction-free survival and overall survival, and treatment complications were monitored.ResultsFifty-four patients with colorectal cancer complicated by obstruction were included. All patients successfully underwent transcatheter arterial infusion combined with lipiodol chemoembolization treatment. The average lipiodol dose administered was 2.62 ± 1.45 ml (0.5–5.5 ml). No serious complications such as perforation or tumor dissemination occurred. The clinical success rate was 83.3% (45/54). One month after treatment, the objective response rate (ORR) and disease control rate (DCR) were 66.67% and 88.9%, respectively. The median obstruction-free survival was 5.0 months. No serious adverse events occurred. As of the last follow-up, 6 patients survived, 44 died, and 4 were lost to follow-up.ConclusionOur findings revealed that transcatheter arterial infusion chemotherapy combined with lipiodol chemoembolization is safe and effective for treating advanced colorectal cancer complicated by obstruction. It may serve as a new treatment strategy for patients with advanced colorectal cancer complicated by obstruction.
Predictors of radiation-induced late rectal toxicity in prostate cancer treatment: a volumetric and dosimetric analysis
Authors: Spohn Simon K. B. , Radicioni Gianluca , Eisfelder Marcio , Zamboglou Constantinos , Baltas Dimos , Grosu Anca-Ligia , Sachpazidis Ilias 
Published: April 2024

IntroductionProstate cancer (PCa) is a prevalent malignancy in European men, often treated with radiotherapy (RT) for localized disease. While modern RT achieves high success rates, concerns about late gastrointestinal (GI) toxicities persist. This retrospective study aims to identify predictors for late GI toxicities following definitive conventionally fractionated external beam RT (EBRT) for PCa, specifically exploring the dose to the rectal wall.Materials and methodsA cohort of 96 intermediate- to high-risk PCa patients underwent EBRT between 2008 and 2016. Rectum and rectum wall contours were delineated, and 3D dose matrices were extracted. Volumetric and dosimetric indices were computed, and statistical analyses were performed to identify predictors using the Mann–Whitney U-rank test, logistic regression, and recursive feature elimination.ResultsIn our cohort, 15 out of 96 patients experienced grade II late proctitis. Our analysis reveals distinct optimal predictors for rectum and rectum wall (RW) structures varying with α/β values (3.0 and 2.3 Gy) across prescribed doses of 68 to 76 Gy. Despite variability, RW predictors demonstrate greater consistency, notably V68Gy[%] to V74Gy[%] for α/β 3.0 Gy, and V68Gy[%] to V70Gy[%] for α/β 2.3 Gy. The model with α/β 2.3 Gy, featuring RW volume receiving 70 Gy (V70Gy[%]), stands out with a BIC value of 62.92, indicating its superior predictive effectiveness. Finally, focusing solely on the rectum structure, the V74Gy[%] emerges the best predictor for α/β 3.0 Gy, with a BIC value of 66.73.ConclusionThis investigation highlights the critical role of V70Gy[%] in the rectum wall as a robust predictor for grade II late gastrointestinal (GI) toxicity following external beam radiation therapy (EBRT) for prostate cancer (PCa). Furthermore, our findings suggest that focusing on the rectum wall specifically, rather than the entire rectum, may offer improved accuracy in assessing proctitis development. A V70Gy (in EQD2 with α/β 2.3 Gy) of ≤5% and if possible ≤1% for the rectal wall should be achieved to minimize the risk of late grade II proctitis.
Does sadness bring myopia: an intertemporal choice experiment with college students
Authors: Lei Peng , Zhang Hao , Zheng Wenyu , Zhang Luoyi 
Published: April 2024

IntroductionWhile economics often interprets individual intertemporal choice preferences through the rationality assumption of utility maximization, the reality is that as emotional beings, individuals’ preferences for intertemporal behavior are much more diverse and inconsistent. Prior research has predominantly focused on positive or negative emotions based on prospect theory, such as anxiety, anger, disgust, and depression. However, there has been relatively little research on how sadness affects individuals’ preferences for immediate and future rewards.MethodsIn this study, 170 college students are recruited as participants, and their emotions are primed with a video before engaging in an intertemporal task. Covariance analysis and logit regression model are established to examine the main and interactive effects of sadness on individuals’ immediate reward preferences.ResultsThe findings reveal that sadness led individuals to prefer smaller immediate rewards, demonstrating a more myopic behavioral pattern, but didn’t affect time discount rate. As the reward baseline increases, sadness’s impact on immediate reward preferences is more pronounced, exacerbating individuals’ myopic behavior.DiscussionIn conclusion, these findings underscore the importance of considering emotional states in economic decision-making models and suggest avenues for future research to explore the complex dynamics of emotions and intertemporal choices.