bluuebunny/crossref_metadata_2025_split huggingface dataset:
TLR4‐dependent induction of vascular adhesion molecule‐1 in rheumatoid arthritis synovial fibroblasts: Roles of cytosolic phospholipase A2α/cyclooxygenase‐2
Authors: Wu Cheng‐Ying , Chi Pei‐Ling , Hsieh Hsi‐Lung , Luo Shue‐Fen , Yang Chuen‐Mao 
Published: January 2010

AbstractLipopolysaccharide (LPS)/Toll‐like receptor 4 (TLR4)‐mediated signaling pathways have caught the attention of strategies designed for rheumatoid arthritis (RA). In this study, we identified that cPLA2α acted as a modulator of LPS‐induced VCAM‐1 expression and THP‐1 (human acute monocytic leukemia cell line) adherence. Treatment of RA synovial fibroblasts (RASFs) with LPS, a TLR4 agonist, promoted the VCAM‐1 expression and THP‐1 adherence which were decreased by pretreatment with a selective cytosolic phospholipase A2 (cPLA2) inhibitor (AACOCF3), implying the involvement of cPLA2α in these responses. This notion was further confirmed by knockdown of cPLA2α expression by transfection with cPLA2α small interfering RNA (siRNA) leading to a decrease in VCAM‐1 expression and THP‐1 adherence induced by LPS. Subsequently, the LPS‐stimulated cPLA2α phosphorylation was attenuated by pretreatment with a MEK1/2 inhibitor (U0126), suggesting that LPS‐stimulated cPLA2α phosphorylation and activity are mediated through an ERK‐dependent mechanism. Moreover, COX‐2‐derived PGE2 production appeared to involve in LPS‐induced VCAM‐1 expression which was attenuated by pretreatment with selective COX‐2 inhibitors (NS‐398 and celecoxib), transfection with COX‐2 siRNA, or PGE2 receptor antagonists. In addition, pretreatment with ecosapentaenoic acid (EPA), a substrate competitor of arachidonic acid (AA), also blocked LPS‐induced VCAM‐1 mRNA and protein expression, and THP‐1 adherence. Collectively, these results suggest that LPS‐induced VCAM‐1 expression and adhesion of THP‐1 cells are mediated through the TLR4/ERK/cPLA2α phosphorylation and COX‐2 expression/PGE2 synthesis in RASFs. J. Cell. Physiol. 223: 480–491, 2010. © 2010 Wiley‐Liss, Inc.
Histone modifications: crucial elements for damage response and chromatin restoration
Authors: Zhu Qianzheng , Wani Altaf A. 
Published: January 2010

AbstractEukaryotic genomes are packaged into chromatin from repeated nucleosome arrays in which DNA sequences wrap around histones. Chromatin organization has profound influence on DNA‐templated processes such as transcription, DNA replication, and repair. Recent studies have also revealed chromatin dynamics as an active contributor to diverse DNA damage responses (DDR). Here, we review recent progress in histone modification related to DDR and post‐repair chromatin restoration at the sites of DNA damage. We discuss how the timing and features of histone modifications would provide the initial as well as the final guidance for DDR, and the prospect that modifications may challenge the epigenetic stability of repaired cells and serve as damage memory in chromatin. J. Cell. Physiol. 223: 283–288, 2010. © 2010 Wiley‐Liss, Inc.
Coactivator recruitment: A new role for PAS domains in transcriptional regulation by the bHLH‐PAS family
Authors: Partch Carrie L. , Gardner Kevin H. 
Published: January 2010

AbstractTranscriptional regulation is dependent on layers of interactions between transcription factors and coactivators, controlling the specificity, temporal regulation, and extent to which transcriptional programs are executed. A key issue in the field of transcriptional regulation is to identify structural mechanisms by which transcription factors and coactivators build hierarchical protein assemblies. The basic helix‐loop‐helix Per‐ARNT‐Sim domain (bHLH‐PAS) family of transcriptional regulators comprises both transcription factors and coactivators, which have different functions despite conserved domain architecture. Within this family, the tandem PAS domains typically mediate dimerization of the transcription factors, while C‐terminal transactivation domains facilitate the dynamic interplay between transcription factors and coactivators. However, recent studies have shown that the modular PAS domains play an important role in regulating coactivator recruitment and oligomerization status. In this study, we provide a brief overview of the structural and functional studies that have identified a novel protein interaction interface on PAS domains utilized by both transcription factors and coactivators within the bHLH‐PAS family. J. Cell. Physiol. 223:553–557, 2010. © 2010 Wiley‐Liss, Inc.
Differences in the secretome of cartilage explants and cultured chondrocytes unveiled by SILAC technology
Authors: Polacek Martin , Bruun Jack‐Ansgar , Johansen Oddmund , Martinez Inigo 
Published: January 2010

AbstractThe main goal of our study was to analyze and compare the profiles of secreted proteins from adult human articular chondrocytes in monolayers, and cartilage explants in culture, using a de novo protein labeling approach. Stable isotope labeling of proteins in culture was used to differentiate between chondrocyte‐derived proteins and other preexisting matrix‐derived components, or proteins coming from serum or synovial fluids. Proteins in culture supernatants were resolved by one‐dimensional SDS‐PAGE electrophoresis, and analyzed in tandem with LC/MS‐MS (liquid chromatography/double mass spectrometry). Results from stable isotope labeling with amino acids in cell culture (SILAC) were validated by specific immunoblotting of four relevant proteins identified in the secretion media. After 8–10 days of culture, over 90% of proteins secreted during monolayer growth contained 13C6‐Arg and 13C6‐Lys. Nonlabeled proteins corresponded mostly to plasma‐associated proteins, indicating background contamination of medium with serum remnants. The majority of the secreted proteins in 2D cultures were extracellular matrix components and matrix regulators, along with some inflammatory agents and metabolic enzymes. In explants, only 25%–30% of proteins were labeled with heavy amino acids, corresponding to matrix regulators and carrier molecules. Nonlabeled proteins corresponded primarily to structural matrix components. In qualitative terms, all labeled proteins coming from cartilage explants were also found in chondrocytes supernatants. In summary, our results show differences in the labeling pattern of proteins found in supernatants from explants and monolayers. Most proteins found in the media of explants were subproducts of matrix turnover rather than newly synthesized. To our knowledge, this study is the first one so far applying SILAC technology in the context of cartilage and chondrocytes physiology. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1040–1049, 2010
Surgery in recurrent epithelial ovarian cancer: Benefits on Survival for patients with residual disease of 0.1–1 cm after secondary cytoreduction
Authors: Tian Wen‐Juan , Jiang Rong , Cheng Xi , Tang Jie , Xing Yan , Zang Rong‐Yu 
Published: January 2010

AbstractAimsRecent retrospective trials stated that a benefit of surgery for recurrent ovarian cancer may be limited to patients in whom a complete cytoreduction (R0) could be achieved. Most of them pointed out there was no difference in survival between residual disease of 0.1–1 cm (R1) and >1 cm (R2). The aim of this study was to evaluate survival benefits from cytoreduction to R1.MethodsBetween 2002 and 2006, 123 patients with recurrent epithelial ovarian cancer undergoing secondary cytoreduction were identified from tumor registry databases.ResultsThe median age at recurrence was 51 years (range: 28–84). Fifty‐one (41.5%) patients had R0, 46 (37.4%) patients had R1, and 26 (21.1%) patients had R2 resection. The median survival of the entire cohort was 31.7 months, with an estimated 5‐year survival of 31.1%. The median survival and estimated 5‐year survival for patients with R1 were 31.1 months and 23.9%, and there were significant differences in survival when compared to 15.6 months and 6.4% in R2 (χ2 = 7.45, P = 0.006), 63.2 months (mean survival) and 54.4% in complete cytoreduction (χ2 = 8.93, P = 0.0028).ConclusionsComplete secondary cytoreduction is the strongest survival determinant in recurrent epithelial ovarian cancer, whereas patients with residual disease of 0.1–1 cm may also benefit from secondary cytoreduction. J. Surg. Oncol. 2010; 101:244–250. © 2010 Wiley‐Liss, Inc.
Recent developments and applications of stir bar sorptive extraction
Authors: Lancas Fernando M. , Queiroz Maria Eugênia C. , Grossi Paula , Olivares Igor R. B. 
Published: January 2010

AbstractThe theoretical aspects of stir bar sorptive extraction (SBSE), as well as the recent applications of this technique in pharmaceutical, biomedical, environmental, and food analysis, and recently developed new coating procedures are reviewed. A general overview of the important factors to be evaluated in the optimization of extraction efficiency such as extraction time, matrix pH, ionic strength, effect of organic additives, temperature, agitation, pre‐extraction derivatization reactions, influence of proteins, and desorption conditions are discussed. An impressive number of applications using SBSE have been published in different areas including biological, environmental, and food, showing the advantages of this technique over the classical extraction techniques (liquid‐liquid extraction (LLE), Soxhlet). Although the different SBSE applications use PDMS phase as sorbent, developments of new phases are necessary for specific applications. In this review, recent SBSE developments are shown with a focus on the development of new instrumental approaches and sorbent phases.
Simultaneous analysis of endogenetic and ectogenic plant hormones by pressurized capillary electrochromatography
Authors: Lu Qiaomei , Zhang Lan , Chen Lihui , Lu Minghua , Tong Ping , Chen Guonan 
Published: January 2010

AbstractPressurized CEC (pCEC) with UV detection was developed for the simultaneous analysis of endogenetic and ectogenic plant hormones. Effects of some crucial factors including mobile phase, buffer concentration, pH value and applied voltage on pCEC separation were investigated, respectively. Under the optimum conditions, namely a packed capillary column (3 μm Hypersil C18, 45 cm total length with 100 μm id×20 cm effective length) as the separation channel; ACN: 10 mmol/L phosphate (50:50, v/v, %) as the mobile phase, pH 4.0; pump flow rate 0.035 mL/min; separation voltage −10 kV; UV wavelength 222 nm, a complete separation of five hormones was accomplished. The pCEC assay presented good linearity (0.8–100 μg/mL), with LOD of 0.2 μg/mL and R in range of 0.998–0.999. Meanwhile, a lack‐of‐fit test was performed to evaluate these linear models. Results showed that no significant lack‐of‐fit was observed and these models were appropriate (α=0.05).
Chemical fingerprint of commercial Radix Echinopsis and quantitative analysis of α‐terthienyl
Authors: Qiao Xue , Han Jian , He Wen‐ni , Wu Tao , Wang Bao‐rong , Ye Min , Guo De‐an 
Published: January 2010

AbstractNew TLC, HPLC and LC/MS methods were developed for the rapid separation, characterization and quantitation of thiophenes in Radix Echinopsis, a herbal medicine, which has been used in China for long history. Nineteen commercial batches of this herb were analyzed by TLC and HPLC. Only five batches were derived from correct species. The other 14 batches were identified as its adulterating species, Radix Rhapontici, as they did not contain characteristic thiophenes of Echinopsis species. By LC/MS, 16 thiophenes were identified from the methanol extracts of Radix Echinopsis and Radix Rhapontici according to their fragmentation behaviors in MS/MS. In addition, a fully validated HPLC method was established to determine the contents of α‐terthienyl in Radix Echinopsis. The contents ranged from 2.7 to 5.2 mg/g for five tested samples. The methods in this paper were simple and reliable, and could be used for the comprehensive quality control of Radix Echinopsis.
Studying the processes during implementation of enterprise systems
Authors: Sedmak Melissa 
Published: January 2010

AbstractThis paper explores the processes that occur during the implementation of an enterprise system (ES). It seeks to identify the most prevalent and/or that which has the most impact on the implementation. Data was collected through a case study of a SAP implementation on the UK site of an international durables manufacturing company. The research questions focus on the method for capturing and analysing process related data, as well as on what the actual processes are. Communication was found to be the most prevalent of processes highlighted by the interviewees. The implications for research and practice are discussed and directions for further research suggested. Copyright © 2010 John Wiley & Sons, Ltd.
diodenlaser in der Solarzellenproduktion
Authors: Horn Wolfgang , Neukum Jörg 
Published: January 2010

AbstractLaser sind in der Produktion von Solarzellen weit verbreitet. So werden z.B. gepulste nd:YAg‐ oder nd:YV04 Laser für die Kantenisolation eingesetzt. Rückseitige elektrische Kontakte durch Laserbohren sowie das Schneiden und Vereinzeln von Siliziumwafern sind ebenfalls akzeptierte Laserverfahren in der Solarzellenproduktion. bei diesen genannten Verfahren wird ein gepulster Laser mit hoher Spitzenleistung und guter Strahlqualität eingesetzt.Hochleistungs‐diodenlaser können zwar nicht mit diesen Parametern aufwarten, bieten jedoch Vorteile wenn kompakte CW‐Strahlquellen mit Fokusgrö�en im Millimeterbereich eingesetzt werden sollen. im Folgenden werden Anwendungen aus dem bereich der Solarzellenproduktion beschrieben, wie das Löten, die Rekristallisierung oder auch die Trocknung. All diese Anwendungen haben gemeinsam, dass gezielt Wärme in den bereich von einigen Quadratmillimetern eingebracht wird.
Effect of Hydration on Silk Film Material Properties
Authors: Lawrence Brian D. , Wharram Scott , Kluge Jonathan A. , Leisk Gary G. , Omenetto Fiorenzo G. , Rosenblatt Mark I. , Kaplan David L. 
Published: January 2010

AbstractEffects of hydration on silk fibroin film properties were investigated for water‐annealed and MeOH‐treated samples. Hydration increased thickness by 60% for MeOH‐immersed films, while water‐annealed samples remained constant. MeOH‐immersed films showed an 80% mass loss due to water, while water‐annealed lost only 40%. O2 permeability was higher in MeOH‐immersed films with Dk values of 10−10 (mL O2 · cm) · (cm−1 · s−1 · mmHg−1), while those of water‐annealed films reached only one fifth of this value. All films showed a decrease in Young's modulus and increased plastic deformation by two orders of magnitude when submerged in saline solution. FT‐IR showed that β‐sheet content in water‐annealed films increased with increasing water vapor pressure, while MeOH‐immersed films showed no change.magnified image
Nano‐Encapsulation of Lipase by Self‐Assembled Nanogels: Induction of High Enzyme Activity and Thermal Stabilization
Authors: Sawada Shin‐ichi , Akiyoshi Kazunari 
Published: January 2010

AbstractThe effects of self‐assembled polysaccharide nanogels on colloidal and thermal stability of lipase from Pseudomonas cepacia were investigated. The enzyme activity, especially kcat, drastically increased in the presence of nanogels of cholesterol‐bearing pullulan (CHP). The thermostability of lipase complex increased because the denaturation temperature of lipase increased by more than 20 °C by complexation with CHP nanogels. Lipase denaturation and aggregation upon heating was effectively prevented by complexation with CHP nanogels. Moreover, complexation with CHP nanogels protected lipase from lyophilization‐induced aggregation. Nano‐encapsulation with CHP nanogel is a useful method for colloidal and thermal stabilization of unstable enzyme.magnified image
Synthesis and Electro‐Optical Properties of a Novel DNA–Lipid Complex Carrying Carbazole Moieties
Authors: Qu Jinqing , Liu Lujing , Chen Huanqin , Ogata Naoya , Masuda Toshio 
Published: January 2010

AbstractA novel DNA–lipid complex carrying carbazole (Cz) moieties was prepared by substituting the sodium counter cation with cationic lipid, namely lipid(2Cz), in which the actual mole ratio of phosphate to lipid was 1:1.05. The DNA–lipid(2Cz) complex was soluble in common organic solvents including CHCl3, CH2Cl2, methanol, and ethanol, while insoluble in THF, toluene, and water. CD spectroscopy revealed that the DNA–lipid complex took a predominantly double helical structure in methanol and that the helical structure was fairly stable against heating. A solution of the DNA–lipid(2Cz) complex emitted fluorescence in 40.0% quantum yield, which was lower than that of the corresponding lipid(2Cz) (76%). The cyclic voltammograms of the complex indicated that the oxidation potential of DNA–lipid(2Cz) was 0.65 V. The onset temperature of weight loss of the DNA–lipid complex is 230 °C according to TGA in air.magnified image
Novel Coiling Behavior in Magnet‐Polymer Composites
Authors: Nguyen Vinh Quang , Ramanujan R. V. 
Published: January 2010

AbstractMagnet‐polymer (Magpol) composites have an interesting ability to undergo large strains in response to an external magnetic field. The contraction behavior of composites of silicone and micron sized iron particles induced by an external magnetic field was studied. Simply by changing boundary conditions, Magpol can exhibit shape change from axial contraction to a novel coiling mechanism due to buckling. The analytically predicted magnetic fields for buckling agreed well with the measured values. The strain versus magnetic field relationship suggested that postbuckling behavior is a stable symmetric bifurcation, which is useful for continuous actuation. The coiling mode of Magpol exhibited high actuation strain (up to 60%) and actuation stress (up to 184 kPa), thus shows good potential for use in soft actuators.magnified image
Microwave Assisted Synthesis of Thiol Modified Polymethacrylic acid and Its Cross‐Linking with Allyl Modified Polymethacrylic acid via Thiol‐ene “Click” Reaction
Authors: Bardts Mareike , Ritter Helmut 
Published: January 2010

AbstractIn this paper, we describe the thiol‐ene “click” reaction with modified poly methacrylic acid in water. The thiol group was implemented by a polymer analogous condensation reaction of polymethacrylic acid (1) and cysteamine (2), which was carried out in bulk by use of microwave. The allyl modified polymethacrylic acid (5) was obtained by DCC‐coupling of the allyl amine onto the polymethacrylic acid backbone. By combination of cysteamine (3) and allyl modified polymethacrylic acid (5) the thiol‐ene click reaction could be started with a redox initiator in aqueous solution at room temperature. The kinetics of this reaction were determined by1H NMR spectroscopy and the resulting hydrogels were analyzed by rheological measurements and differential scanning calorimetry (DSC).magnified image
Poly(lactic acid) Toughening with a Better Balance of Properties
Authors: Rasal Rahul M. , Hirt Douglas E. 
Published: January 2010

AbstractPoly(lactic acid) (PLA) toughening is often associated with significant modulus and/or strength losses making it unsuitable for many consumer and biomedical applications. The major objective of this research was to toughen PLA without significant loss in modulus and strength and to introduce reactive acid groups using reactive blending of PLA with a combination of polymers. PLA was reactive blended with poly(acrylic acid) (PAA) followed by physical blending with poly(ethylene glycol) (PEG) in solution. The modified PLA was extruded into films using a co‐rotating twin‐screw extruder and characterized using tensile testing, differential scanning calorimetry (DSC), and dynamic mechanical analyses (DMA). This technology resulted in films with a ten‐fold increase in toughness compared to neat PLA with little or no decrease in strength and modulus.magnified image
Functional correlates of lower cognitive test scores in essential tremor
Authors: Louis Elan D. 
Published: January 2010

AbstractAlthough motor features have been the defining element of essential tremor (ET), lower neurocognitive test scores are increasingly being recognized. However, the clinical correlates, if any, of these lower test scores remain largely unexplored. The aim of this study was to determine whether cognitive test scores in ET have any functional correlates. The Modified Mini Mental Status Examination (MMSE), Katz Activities of Daily Living (ADL) scale and Lawton Instrumental (I) ADL scale were administered to 95 cases. The Katz ADL score (rho = 0.26, P = 0.01) and Lawton IADL score (rho = 0.32, P = 0.001) were correlated with MMSE scores, such that poorer cognitive performance indicated greater dysfunction. Furthermore, cognitive test scores were a better predictor of functional disability than was tremor severity. Poorer cognitive performance in ET was associated with greater functional deficit. Cognition should enter the clinical dialog with ET patients as an issue of clinical significance. © 2010 Movement Disorder Society
Narratives, facts, and events in the foundations of information science
Authors: Buckland Michael , Dousa Thomas M. , Shaw Ryan 
Published: January 2010

AbstractThis session explores three basic notions: events, facts, and narratives.Events play a large role in our lives. Our sense of identity is largely shaped by events we have experienced. Our understanding of history is a narrative of events in the past. The humanities and social sciences are concerned with the human experience, with actions and interactions. The sciences also deal with events: changes, processes, and experiments. Events may be described factually and themselves be regarded as facts. Facts, in turn, assume their full meaning only in relation to narratives, which provide the contextual frameworks for understanding them.In our daily lives we have little difficulty thinking and talking about events, facts, and narratives. However, information systems operate on objects (bits, data, documents). Events, facts, and narratives are not objects and difficulties arise when we try to analyze formally what they are.Three related papers address these issues. First, a direct examination of the nature of events; second, an account of two contrasting ways of contextualizing facts; and, third., an attempt to encode events in biographical narratives.
Information seeking and use in diverse organizational contexts
Authors: Detlor Brian , Choo Chun Wei , Mackenzie Maureen , Turnbull Don , Ratto Matt 
Published: January 2010

AbstractThis session combines individual presentations with a group discussion. The focus of this session and the expertise of this panel bring together ways of thinking about information seeking and use in diverse organizational contexts. Organizational contexts are not uniform. Quite the contrary, they are very diverse in terms of the individuals, cultures, habits, routines, systems and infrastructures within them. The panelists offer varying viewpoints on how to best address information seeking and use diversity in the workplace. Though each panelist offers different perspectives on how to do this, collectively they rally a persuasive need to not assume homogeneity in our understanding and investigation of information seeking and use in organizational settings. Rather, they suggest it would be better to recognize the acute diversity in the individuals, tasks, cultures, technologies, and information practices that exist in organizations today, and to develop models, approaches, and recommendations of information seeking and use that reflect our understanding of this diversity.