bluuebunny/crossref_metadata_2025_split huggingface dataset:
The effects of flubendazole and its metabolites on the larval development of Haemonchus contortus (Nematoda: Trichostrongylidae): an in vitro study
Authors: Bártíková H. , Skálová L. , Lamka J. , Szotáková B. , Várady M. 
Published: December 2010

Abstract
The anthelmintic effects of flubendazole (FLU), its two main metabolites reduced flubendazole (FLU-R) and hydrolyzed flubendazole (FLU-H), and thiabendazole (TBZ) were compared using an in vitro larval development test in two isolates of Haemonchus contortus, a fully susceptible isolate (HCS) and a multi-resistant isolate (HCR). Results were quantified as 50 % lethal concentration (LC50), 99 % lethal concentration (LC99), efficacy factor (EF), and resistance factor (RF). For HCS, both LC50 and LC99 of FLU were lower than those of the reference TBZ. The anthelmintic activity of FLU-R in HCS and HCR was 13 and 6 times lower than the activity of FLU, respectively. The anthelmintic activity of FLU-H was negligible (approximately 363–853 times lower) compared to that of FLU. Although a marked resistance of the HCR isolate to TBZ was confirmed, only a low tolerance to FLU-R and slightly higher tolerance to FLU were found.
Modern Diachronic Corpus-Assisted Discourse Studies (MD-CADS) on UK newspapers: an overview of the project
Authors: Partington Alan 
Published: December 2010

This edition of Corpora contains one of the first ever collections of papers pertaining to the nascent discipline of Modern Diachronic Corpus-Assisted Discourse Studies (MD-CADS). This discipline is characterised by the novelty both of its methodology and the topics it is, consequently, in a position to treat. It employs relatively large corpora of a parallel structure and content from different moments of contemporary time (in this case the SiBol corpora, see below) in order to track changes in modern language usage but also social, cultural and political changes as reflected in language.In this overview, I will attempt to give an idea of what both corpus-assisted discourse studies (CADS) and MD-CADS involve, to provide some information about the newspaper corpora we employ, and to outline methodologies commonly followed in this area, including those employed by the other contributors to this issue. I will also present two sets of practical analyses. The first is inductive and bottom-up, derived from a close analysis of the comparative keywords generated by comparing the lists of items from the two parallel corpora from different time periods; the aim is to uncover changes over time both in language and in what social, political and cultural issues were considered worthy of attention. The second is more intuitive and hypothesis-driven; the hypothesis is that an examination of a certain term, namely moral panic, can shed some light on which issues writers thought did not merit all the attention they were receiving. I will conclude with brief sketches of the other papers in this issue, and reflections on the relevance of MD-CADS in both language research and teaching.
Candidate Gene Association Study of Magnetic Resonance Imaging-based Hip Osteoarthritis (OA): Evidence for COL9A2 Gene as a Common Predisposing Factor for Hip OA and Lumbar Disc Degeneration
Authors: NÄKKI ANNU , VIDEMAN TAPIO , KUJALA URHO M. , SUHONEN MATTI , MÄNNIKKÖ MINNA , PELTONEN LEENA , BATTIÉ MICHELE C. , KAPRIO JAAKKO , SAARELA JANNA 
Published: December 2010

Objective.To study whether gene variants associated with lumbar disc degeneration (LDD) phenotypes are also associated with hip osteoarthritis (OA).Methods.Magnetic resonance imaging (MRI)-based hip OA changes for 345 twins were assessed and 99 single-nucleotide polymorphisms (SNP) were analyzed.Results.Variants in the COL9A2 (rs7533552, p = 0.0025) and COL10A1 (rs568725, p = 0.002) genes showed association with hip OA.Conclusion.The associating G allele in COL9A2 changes a glutamine to arginine or to tryptophan and may predispose to both hip OA and LDD, making it a candidate for degenerative connective tissue diseases.
Association ofEMCNwith Susceptibility to Rheumatoid Arthritis in a Japanese Population
Authors: NISHIMOTO KAZUMASA , IKARI KATSUNORI , KANEKO HIROTAKA , TSUKAHARA SO , KOCHI YUTA , YAMAMOTO KAZUHIKO , NAKAMURA YUSUKE , TOYAMA YOSHIAKI , TANIGUCHI ATSUO , YAMANAKA HISASHI , MOMOHARA SHIGEKI 
Published: December 2010

Objective.Endomucin, an endothelial-specific sialomucin, is thought to facilitate “lymphocyte homing” to synovial tissues, resulting in the major histopathologies of rheumatoid arthritis (RA). We examined the association between RA susceptibility and the gene coding endomucin,EMCN.Methods.Association studies were conducted with 2 DNA sample sets (initial set of 1504 patients, 752 controls; and validation set, 1113 patients, 940 controls) using 6 tag single-nucleotide polymorphisms (SNP) from the Japanese HapMap database. Immunohistochemistry for the expression of endomucin was conducted with synovial tissues from 4 patients with RA during total knee arthroplasty. Electromobility shift assays were performed for the functional study of identified polymorphisms.Results.Within the initial sample set, the strongest evidence of an association with RA susceptibility was SNP rs3775369 (OR 1.20, p = 0.0075). While the subsequent replication study did not initially confirm the observed significant association (OR 1.13, p = 0.062), an in-depth stratified analysis revealed significant association in patients testing positive to anti-cyclic citrullinated peptide (anti-CCP) antibody in the replication data set (OR 1.15, p = 0.044). Investigating 2 sample sets, significant associations were detected in overall and stratified samples with anti-CCP antibody status (OR 1.17, p = 0.0015). Positive staining for endomucin was detected in all patients. The allele associated with RA susceptibility had a higher binding affinity for HEK298-derived nuclear factors compared to the nonsusceptible allelic variant of rs3775369.Conclusion.A significant association betweenEMCNand RA susceptibility was detected in our Japanese study population. TheEMCNallele conferring RA susceptibility may also contribute to the pathogenesis of RA.
Cortisol During the Day in Patients with Systemic Lupus Erythematosus or Primary Sjögren’s Syndrome
Authors: van der GOES MARLIES C. , BOSSEMA ERCOLIE R. , HARTKAMP ANDRÉ , GODAERT GUIDO L.R. , JACOBS JOHANNES W.G. , KRUIZE AIKE A. , DERKSEN RONALD H.W.M. , BIJLSMA JOHANNES W.J. , GEENEN RINIE 
Published: December 2010

Objective.To compare the level and change of cortisol during the day of patients with systemic lupus erythematosus (SLE) and primary Sjögren’s syndrome (pSS) with low and high erythrocyte sedimentation rate (ESR).Methods.Saliva was collected in the real-life environment of 21 women with SLE, 16 women with pSS, and 30 age-matched healthy women at 9 fixed timepoints during 2 consecutive days. Repeated measures ANOVA was performed to examine whether cortisol levels during the day were different for the patients with low ESR (≤ 20 mm/h) versus those with high ESR (> 20 mm/h).Results.The groups with low and high ESR showed the characteristic change of cortisol during the day (time-of-day effect, F = 124.9, p < 0.001). The cortisol awakening level was lower for patients with high ESR than for patients with low ESR (group*time effect, F = 3.1, p = 0.02).Conclusion.The cortisol awakening level differs for patients with low and high ESR, which indicates the usefulness of further studies of hypothalamic-pituitary-adrenal axis dynamics in patients with SLE and pSS.
CD101 Expression and Function in Normal and Rheumatoid Arthritis-affected Human T Cells and Monocytes/Macrophages
Authors: JOVANOVIC DRAGAN V. , BOUMSELL LAURENCE , BENSUSSAN ARMAND , CHEVALIER XAVIER , MANCINI ARTURO , DI BATTISTA JOHN A. 
Published: December 2010

Objective.It was recently reported that CD101 surface expression discriminates potency among CD4+CD25+ FoxP3+ regulatory T cells in the mouse. We investigated whether CD101 may also have a role in the suppressor function of regulatory T cells in humans given that the latter population may affect the autoimmune response in patients with rheumatoid arthritis (RA).Methods.Sorted T cells and monocyte/macrophage cell populations were analyzed by flow cyto metry using conjugated antibodies specific for cell-surface markers. T cell proliferation assays were conducted by [3H]thymidine incorporation and CD8highcytotoxicity measurements by Cyto-Scan-LDH cytotoxicity assays. ELISA were used to measure cytokines in cell culture supernatants and Western blotting was performed for profiling mitogen-activated protein (MAP) kinase activation using specific antiphospholipid antibodies.Results.CD101 expression coincided with PMA-induced monocyte/leukocyte lineage differentiation. CD8highCD101− T cells exhibited greater cytotoxic activity than CD8highCD101+ T cells, while no difference was observed between CD4CD25highCD101+ and CD4CD25highCD101− Treg inhibitory activity through responder T cells. LPS-induced proinflammatory cytokine production and p38 MAP kinase activation were made possible by ligation of CD101 with an anti-CD101 antibody F(ab’)2fragment.Conclusion.These results suggested a modulatory/coregulatory function of CD101 in the human immune system, in contrast to murine models, in which CD101 surface expression discriminates potency among FoxP3+ regulatory T cells. Cytotoxic CD8highCD101+ T cells were markedly less cytotoxic than CD8highT cells negative for the CD101 antigen and were conspicuously downregulated in patients with RA, suggesting a possible role for CD101 expression and function in the control of certain manifestations of RA pathology.
Clinical and Radiological Outcomes of Axial Lumbar Interbody Fusion
Authors: Patil Suresh S. , Lindley Emily M. , Patel Vikas V. , Burger Evalina L. 
Published: December 2010

Axial lumbar interbody fusion is a novel percutaneous alternative to common open techniques, such as anterior, posterior, and transforaminal lumbar interbody fusion. This minimally invasive technique uses the presacral space to access the L5-S1 and L4-L5 disk space. The goal of this study was to examine outcomes following axial lumbar interbody fusion.
The charts of all patients who underwent axial lumbar interbody fusion surgery at our institution between 2006 and 2008 were reviewed. Clinical outcomes included visual analog scale (VAS) and Oswestry Disability Index (ODI). Radiographs were also evaluated for disk space height, L4-L5 and/or L5-S1 Cobb angle, and fusion. Of the 50 patients (32 women, 18 men; mean age, 49.29 years) treated with axial lumbar interbody fusion, 48 had preoperative VAS scores and 16 had preoperative ODI scores available. Complete radiographic data were available at the preoperative, initial postoperative, and final postoperative time points for 46 patients (92%). At last follow-up (average, 12 months), ODI scores were reduced from 46 to 22, and VAS scores were lowered from 8.1 to 3.6. Of the 49 patients with postoperative radiographs, 47 (96%) went on to a solid fusion. There were no significant differences between pre- and postoperative disk space height and lumbar lordosis angle. The most common complications were superficial infection and pseudoarthrosis. Other complications were rectal injury, hematoma, and irritation of a nerve root by a screw.
Overall, we found the axial lumbar interbody fusion procedure in combination with pedicle screw placement to have good clinical and radiological outcomes.
Effect of Ipsilateral C7 Nerve Root Transfer on Restoration of Rat Upper Trunk Muscle and Nerve Function After Brachial Plexus Root Avulsion
Authors: Song Jie , Chen Liang , Gu Yudong 
Published: December 2010

The effects of ipsilateral cervical nerve root transfer on the restoration of the rat upper trunk muscle and nerve brachial plexus root avulsion were studied. After simulated root avulsion of the upper trunk brachial plexus, 120 rats were randomly divided into 4 groups: (A) ipsilateral C7 root transfer group; (B) Oberlin group; (C) phrenic nerve group; and (D) no axillary nerve restoration group. At 3, 6, and 12 weeks postoperatively, Ochiai score, Barth feet overreaching test, Terzis grooming test, and indices of neurotization were determined in 10 rats from each group.
Twelve weeks postoperatively, nearly all the behavioral, neuroelectrophysiological, and histological outcomes of the axillary nerve and deltoid muscle and some of the indices of musculocutaneous nerve and biceps brachii function in the ipsilateral C7 group were superior to those in the other 3 groups. No significant difference was found between the ipsilateral C7 group and the other 3 groups in recovery rate of wet biceps muscle weight. No significant difference was found between the ipsilateral C7 group and the Oberlin group in the recovery of the axillary nerve compound muscle action potential and biceps brachii cell size. No significant difference was found between the ipsilateral C7 group and the phrenic nerve and no axillary nerve restoration groups in amplitude recovery rate of musculocutaneous nerve compound muscle action potential. No significant difference was found between the ipsilateral C7 and the Oberlin groups in the early recovery of musculocutaneous nerve compound muscle action potential, but recovery was significantly better in the ipsilateral C7 group at 12 weeks.
Ipsilateral C7 root transfer can improve the quality of restoration of muscle and nerve function in the rat upper trunk after brachial plexus root avulsion.
Bilateral PCL Hypoplasia Resulting in Posterior, Posterolateral Rotatory Instability and Tears of the Lateral Meniscus Anterior Horn
Authors: Lee Yong Seuk , Chun Dong-Il , Park Min Jung 
Published: December 2010

This article describes a case of bilateral sagged knees presenting as posterior, posterolateral rotatory instability with tears of the anterior horn of the lateral meniscus. Each knee had identical tears of the lateral meniscus anterior horn. A 42-year-old woman reported bilateral anterior knee pain and painful instability during running or jarring exercises. She reported no major trauma to her knees. Arthroscopic findings of her right knee revealed a posterior cruciate ligament that looked hypoplastic but was without acute injury, and the anterior horn of the lateral meniscus showed chronic complex tears with some degeneration. Posterior cruciate ligament reconstruction, posterolateral corner sling, and meniscal repair of the lateral meniscus anterior horn was performed on her right knee. Three months later, a similar operation was performed on her left knee. However, menisectomy was performed because the lateral meniscus anterior horn tear was in the junction of the red-white and white zones.At 18 months postoperatively, the patient reported no symptoms and was satisfied with her results. Physical examination showed no joint line tenderness, and posterior stress radiographs on both knees showed grade I posterior instability. She showed no posterolateral subluxaion by supine dial test, and her prone dial test also improved approximately 15° on both knees. Lysholm score was 74 preoperatively and improved to 92 postoperatively.
Transverse Femoral Implant Prominence: Four Cases Demonstrating a Preventable Complication for ACL Reconstruction
Authors: Argintar Evan , Scherer Benjamin , Jordan Tom , Klimkiewicz John 
Published: December 2010

Anterior cruciate ligament (ACL) tear is a commonly occurring injury that often demands surgical reconstruction. Although the utility of this operation is widely accepted, many specific components, including graft fixation technique, remain controversial. Many clinicians favor transverse femoral implant fixation for soft tissue ACL grafts. This technique can be accomplished successfully; however, in a minority of the cases, the femoral implant can be excessively prominent, leading to iatrogenic postoperative iliotibial band syndrome.
This article presents 4 patients that developed postoperative iliotibial band syndrome resulting from transverse femoral implant prominence. Despite achievement of knee ligamentous stability, implant prominence compromised final clinical results following ACL reconstruction. Through change in Lysholm value, we reviewed the clinical outcomes of these patients following femoral implant hardware removal for treatment of iliotibial band syndrome. On hardware removal, all patients demonstrated complete symptomatic improvement, mirroring an average Lysholm value increase of 38. We believe transverse femoral implant prominence is avoidable, and subsequent iliotibial band syndrome is a preventable postoperative complication.
Unilateral Cervical Facet Dislocation in a 9-Year-Old Boy
Authors: Parada Stephen A. , Arrington Edward D. , Kowalski Kurtis L. , Molinari Robert W. 
Published: December 2010

Facet dislocations in children are rare. This article presents the youngest case of a unilateral facet dislocation described in the pediatric population. A 9-year-old boy sustained a flexion/axial loading injury to his cervical spine while wrestling with his friends, causing a unilateral facet dislocation at C4/5. Prereduction magnetic resonance imaging (MRI) demonstrated the absence of a diskal herniation or cord impingement and an intact posterior ligamentous complex. Due to the patient’s young age, the decision was made to forgo a supervised awake closed reduction. Closed reduction was performed under general anesthesia with somatosensory-evoked potential and motor-evoked potential monitoring. Closed reduction was successfully achieved after Gardner-Wells tongs were applied and used to manually direct slow, steady in-line traction, along with slight flexion and posterior rotation of the dislocated side under direct fluoroscopy.
The patient was immediately awakened from anesthesia and was found to have an intact sensory examination. He was immobilized in a cervical collar for 12 weeks. At 2-year follow-up, he remained asymptomatic without recurrence and had painless full range of motion of the cervical spine. Radiographs revealed a normally aligned cervical spine.
Unilateral cervical facet dislocations and subluxations are the result of a distractionflexion force applied to the spine along with a rotational component. These are not uncommon injuries in the adult spine; however, in the young pediatric population, cervical facet dislocations are rare.
Mass-Induced Unilateral Hallux Valgus
Authors: Young Ki Won , Lee Kyung Tai , Kwak Jeong Ja , Lee Young Koo , Park Young Uk 
Published: December 2010

Various causes of hallux valgus exist. However, mass-induced hallux valgus is a rare phenomenon. To the best of our knowledge, there has been 1 previous report on mass-induced hallux valgus in the English literature; however, that report described giant cell tumor of the extensor digitorum longus tendon sheath. We believe ours to be the first published case of osteochondroma of the second metatarsus-induced hallux valgus.
A 19-year-old woman presented with pain and tenderness in the left hallux of 3 months’ duration. After a diagnosis of bony mass with hallux valgus, an operative excision was performed through a dorsal approach on the first web space of the left foot. The histopathologic diagnosis was osteochondroma. One-year follow-up radiographs showed a decreased hallux valgus angle and intermetatarsal angle.
Unilateral hallux valgus occurs in a mass area. After excision of the mass, hallux valgus angle and intermetatarsal angle decrease. The rotational deformity is not severe. There is little probability of discovering its etiology, but mass-like osteochondroma should be considered.
Efficacy of Corneal Collagen Cross-Linking Using a Custom Epithelial Debridement Technique in Thin Corneas: A Confocal Microscopy Study
Authors: Kaya Vedat , Utine Canan Asli , Yilmaz Omer Faruk 
Published: December 2010

Purpose:
To examine the corneal collagen cross-linking (CXL) effect with a customized epithelial debridement technique in thin corneas using anterior segment optical coherence tomography (OCT) and confocal microscopy.

Methods:
Two patients with keratoconus who underwent a customized epithelial debridement technique (preserving the epithelium in thinner corneal regions where the deepithelialized corneal thickness was <400 μm) and subsequent CXL were examined by anterior segment OCT and in vivo confocal microscopy 1 month postoperatively.

Results:
Anterior segment OCT showed the demarcation line in the peripheral deepithelialized corneal areas, but it was not detectable in the central corneal regions corresponding to the cone where the epithelium was preserved. In vivo confocal microscopy analysis revealed total loss of keratocytes together with the intense honeycomb-like stromal edema in the anterior ∼320 μm of the cornea in the deepithelialized areas. This effect was mild under the intact epithelium with preservation of keratocytes and only a mild increase in stromal reflectivity.

Conclusions:
In this confocal microscopy study, the corneal stroma under the intact epithelium seemed to be spared from the collagen CXL effect by the customized epithelial debridement technique.
Lipid Raft Assembly and Lck Recruitment in TRAIL Costimulation Mediates NF-κB Activation and T Cell Proliferation
Authors: Huang Shih-Chia , Tsai Hwei-Fang , Tzeng Horng-Tay , Liao Hsiu-Jung , Hsu Ping-Ning 
Published: December 2010

Abstract
The TNF-related apoptosis-inducing ligand was shown to provide a costimulatory signal that cooperates with the TCR/CD3 complex to induce T cell proliferation and cytokine production. Although a number of signaling pathways were linked to the TCR/CD3 complex, it is not known how these two receptors cooperate to induce T cell activation. In this study, we show that TRAIL-induced costimulation of T cells depends on activation of the NF-κB pathway. TRAIL induced the NF-κB pathway by phosphorylation of inhibitor of κB factor kinase and protein kinase Cθ in conjunction with anti-CD3. Furthermore, we demonstrated that TRAIL costimulation induced phosphorylation of the upstream TCR-proximal tyrosine kinases, Lck and ZAP70. Ligation of the TRAIL by its soluble receptor, DR4-Fc, alone was able to induce the phosphorylation of Lck and ZAP70 and to activate the NF-κB pathway; however, it was insufficient to fully activate T cells to support T cell proliferation. In contrast, TRAIL engagement in conjunction with anti-CD3, but not TRAIL ligation alone, induced lipid raft assembly and recruitment of Lck and PKCθ. These results demonstrate that TRAIL costimulation mediates NF-κB activation and T cell proliferation by lipid raft assembly and recruitment of Lck. Our results suggest that in TRAIL costimulation, lipid raft recruitment of Lck integrates mitogenic NF-κB–dependent signals from the TCR and TRAIL in T lymphocytes.