bluuebunny/crossref_metadata_2025_split huggingface dataset:
Discourses of ISDS reform: a comparison of UNCITRAL Working Group III and ICSID processes
Authors: Marcoux Jean-Michel , Bjorklund Andrea K , Whitsitt Elizabeth A , Vanhonnaeker Lukas 
Published: May 2024

Abstract
The reform of investor-state dispute settlement (ISDS) has been tackled by the International Centre for Settlement of Investment Disputes (ICSID) and United Nations Commission on International Trade Law (UNCITRAL) Working Group (WG) III. Despite different objectives, both processes have relied on written submissions from various stakeholders. What are the structures and the narratives underlying the discourses of ISDS reform in these organizations? This article explores the content of 172 submissions by using mixed methods. It demonstrates that UNCITRAL WG III has involved less structured submissions whose content has expanded the initial mandate, with narratives encapsulating deeper disagreement among participants. By contrast, ICSID operated through a common pattern across submissions and a stronger focus on procedural issues, with less disagreement revealed in its narratives. The article proceeds in three steps. First, it compares the structure of discourses for each reform process by aggregating the content of submissions through computational analysis. Second, it relies on critical discourse analysis to reveal narratives that have emerged in each process. Lastly, the article explores submissions from actors who have participated in both processes to illustrate how they have navigated the tension between structures and narratives when reforming international investment arbitration.
Cytokinin oxidase/dehydrogenase inhibitors: progress towards agricultural practice
Authors: Nisler Jaroslav , Klimeš Pavel , Končitíková Radka , Kadlecová Alena , Voller Jiří , Chalaki Mahfam , Karampelias Michael , Murvanidze Nino , Werbrouck Stefaan P O , Kopečný David , Havlíček Libor , De Diego Nuria , Briozzo Pierre , Moréra Solange , Zalabák David , Spíchal Lukáš 
Published: May 2024

Abstract
Cytokinin oxidase/dehydrogenase (CKX) inhibitors reduce the degradation of cytokinins in plants and thereby may improve the efficiency of agriculture and plant tissue culture-based practices. Here, we report a synthesis and structure–activity relationship study of novel urea derivatives concerning their CKX inhibitory activity. The most active compounds showed sub-nanomolar IC50 values with maize ZmCKX1, the lowest value yet documented. Other CKX isoforms of maize and Arabidopsis were also inhibited very effectively. The binding mode of four compounds was characterized based on high-resolution crystal complex structures. Using the soil nematode Caenorhabditis elegans, and human skin fibroblasts, key CKX inhibitors with low toxicity were identified. These compounds enhanced the shoot regeneration of Lobelia, Drosera, and Plectranthus, as well as the growth of Arabidopsis and Brassica napus. At the same time, a key compound (identified as 82) activated a cytokinin primary response gene, ARR5:GUS, and a cytokinin sensor, TCSv2:GUS, without activating the Arabidopsis cytokinin receptors AHK3 and AHK4. This strongly implies that the effect of compound 82 is due to the up-regulation of cytokinin signalling. Overall, this study identifies highly effective and easily prepared CKX inhibitors with a low risk of environmental toxicity for further investigation of their potential in agriculture and biotechnology.
Towards determinism in PDL: relations and proof theory
Authors: Benevides Mario , Gomes Leandro , Lopes Bruno 
Published: May 2024

Abstract
Guarded Kleene Algebra with Tests (GKAT) was presented as a fragment of KAT to abstract imperative programming languages, where only if-then-else and while-do statements are allowed in the language. The loss of expressiveness is, nevertheless, compensated by a clear advantage over KAT: it allows almost linear decidability of program equivalence. In this work, we give the first step to optimizing the complexity of dynamic logic equivalence, which is EXPTIME-complete in propositional dynamic logic (PDL). First, and based on strict deterministic PDL, we present guarded propositional dynamic logic (GPDL), a fragment of PDL where programs correspond to GKAT terms. It comes embedded, as expected, with a semantics over relational models and a sound axiomatisation. Then, we present a Natural Deduction system for GPDL, proving its soundness and completeness, concerning the axiomatisation. Based on Smolka et al. (2019, Proceedings of the ACM Programming Language 4), we obtain the main result of this work—the equivalence of GPDL programs can be established in almost linear time.
Usefulness of an Ultrasound-guided Prehospital Care for SCUBA Diving Accidents
Authors: Martinet Camille , Cungi Pierre-Julien , Morin Jean 
Published: May 2024

ABSTRACT
The incidence of diving accidents is increasing. Point-of-care ultrasound is the only imaging tool available in the field for the military physician who practices in isolated conditions. While ultrasound is integrated in the pre-hospital evaluation protocols of severe trauma patients, few applications are described for diving accident victims. Through a clinical case, we propose an algorithm of ultrasound triage for diving accidents with pulmonary symptoms. Point-of-care ultrasound makes it possible to avoid a risky transfer, by supporting a diagnosis and the treatment on the spot, to rule out contraindications to recompression, and to detect and monitor the treatment of high-risk complications such as the capillary leak syndrome.
Irritable Bowel Syndrome in Veterans With Gulf War Illness Evaluated at VA’s War-Related Illness and Injury Study Center
Authors: Nono Djotsa Alice B S , Nguyen Wenker Theresa H , Ahmed Sarah T , Ghosh Saurendro , Malhotra Deeksha , Boyle Stephen H , Gifford Elizabeth J , Sims Kellie J , White Donna L , Steele Lea , Helmer Drew A 
Published: May 2024

ABSTRACT

Introduction
Excess rates of Gulf War illness (GWI) and irritable bowel syndrome (IBS), two chronic multisymptom illnesses, have long been documented among nearly 700,000 veterans who served in the 1990-1991 Persian Gulf War. We sought to report the prevalence, characteristics, and association of GWI and IBS decades after the war in a clinical cohort of deployed Gulf War veterans (GWVs) who were evaluated at the Department of Veterans Affairs’ War Related Illness and Injury Study Center (WRIISC) for unexplained chronic symptoms.

Materials and Methods
We analyzed data gathered from clinical intake questionnaires of deployed GWVs who were evaluated at WRIISC clinics between 2008 and 2020. We applied Centers for Disease Control (CDC) criteria to determine the prevalence of severe GWI. IBS was identified using Rome IV diagnostic criteria (current IBS) and veterans’ self-reported “history of physician-diagnosed IBS.” We examined associations between IBS and GWI using bivariate analyses and multivariable logistic regression.

Results
Among the N = 578 GWVs evaluated by the WRIISC, severe GWI (71.8%), history of physician-diagnosed IBS (50.3%) and current IBS (42.2%) were all highly prevalent. Nearly half of GWVs with severe GWI met Rome criteria for IBS (45.8%), and over half reported a history of physician-diagnosed IBS (56.1%). In multivariable models, severe GWI was significantly associated both with current IBS (adjusted odds ratio (aOR): 1.68, 95% CI: 1.11, 2.54) and with veteran-reported history of physician-diagnosed IBS (aOR: 2.15, 95% CI: 1.43, 2.23). IBS with diarrhea (IBS-D) was the most common subtype among GWVs with current IBS (61.1%). However, IBS-mixed affected a significantly greater proportion of veterans with severe GWI, compared to veterans who did not have severe GWI (P = .03).

Conclusions
More than 20 years after the Persian Gulf War, our findings indicate a high degree of comorbidity between severe GWI and IBS among deployed GWVs seeking care for unexplained illnesses. Our results suggest GWVs with GWI should be screened for IBS for which evidence-based treatments are available and could potentially reduce symptom burden. Conversely, symptoms of IBS should trigger additional evaluation for non-gastrointestinal symptoms in deployed Gulf War veterans to identify possible GWI and ensure a comprehensive approach to care.
Candidates for downstream jets at interplanetary shocks
Authors: Hietala H , Trotta D , Fedeli A , Wilson L B , Vuorinen L , Coburn J T 
Published: May 2024

ABSTRACT
Localized dynamic pressure enhancements arising from kinetic processes are frequently observed downstream of the Earth’s bow shock. These structures, called jets, modify their plasma surroundings and participate in particle energization. Here, we report the first observations of jet-like structures in a non-planetary shock environment: downstream of interplanetary shocks. We introduce an analysis approach suitable for such conditions and apply it to Wind spacecraft data. We present one event with a Mach number similar to the Earth’s bow shock as a benchmark, as well as two low Mach number, low beta shocks: a parameter range that is difficult to access at planets. The jet-like structures we find are tens of ion inertial lengths in size, and some are observed further away from the shock than in a limited magnetosheath. We find that their properties are similar to those of magnetosheath jets: in the frame of the shock these structures are fast, cold, and most have no strong magnetic field variations. All three interplanetary shocks feature foreshock activity, but no strongly compressive waves. We discuss the implications, these findings have for the proposed jet formation mechanisms.
Orbit determination of asteroid (469219) Kamo‘oalewa using a combination of historical and new observations
Authors: Huang Hao , Liu Shanhong , Ge Liang , Cao Jianfeng , Li Xie , Gao Jian 
Published: May 2024

ABSTRACT
The orbit solution of near-Earth asteroids heavily relies on ground-based optical observations. The orbit uncertainty is limited by the insufficient observation quantity and quality. The Chinese Tianwen-2 mission targets a near-Earth asteroid (469219) Kamo‘oalewa and a main-belt comet, 311P/PANSTARRS. To accurately determine the orbit of Kamo‘oalewa, more optical observations are needed. Autonomous observation experiments focusing on Kamo‘oalewa were carried out using the 2.16-m telescope at the Xinglong Observatory of the National Astronomical Observatory of China. We found that this telescope could observe near-Earth asteroids as faint as a magnitude of 22.8. Based on the stacking method and trailed star extraction algorithm, 14 optical observations were obtained from 2022 to 2024. The orbit for Kamo‘oalewa was determined by combining our observations with historical observations between 2004 and 2024. The inclusion of our observations results in improved orbital uncertainties of Kamo‘oalewa by 18.67, 7.93, and 11.12 km (1σ) in the X, Y, and Z directions, respectively. Furthermore, by combining all existing and simulated observations, the uncertainty of the orbital determination of Kamo‘oalewa was analysed. When using an additional 180 group observations from 3 Chinese observatories over 2 yr, the orbital uncertainties of Kamo‘oalewa in the 3 directions could be reduced to 30 km (1σ).
New Drosophila promoter-associated architectural protein Mzfp1 interacts with CP190 and is required for housekeeping gene expression and insulator activity
Authors: Sokolov Vladimir , Kyrchanova Olga , Klimenko Natalia , Fedotova Anna , Ibragimov Airat , Maksimenko Oksana , Georgiev Pavel 
Published: May 2024

Abstract
In Drosophila, a group of zinc finger architectural proteins recruits the CP190 protein to the chromatin, an interaction that is essential for the functional activity of promoters and insulators. In this study, we describe a new architectural C2H2 protein called Madf and Zinc-Finger Protein 1 (Mzfp1) that interacts with CP190. Mzfp1 has an unusual structure that includes six C2H2 domains organized in a C-terminal cluster and two tandem MADF domains. Mzfp1 predominantly binds to housekeeping gene promoters located in both euchromatin and heterochromatin genome regions. In vivo mutagenesis studies showed that Mzfp1 is an essential protein, and both MADF domains and the CP190 interaction region are required for its functional activity. The C2H2 cluster is sufficient for the specific binding of Mzfp1 to regulatory elements, while the second MADF domain is required for Mzfp1 recruitment to heterochromatin. Mzfp1 binds to the proximal part of the Fub boundary that separates regulatory domains of the Ubx and abd-A genes in the Bithorax complex. Mzfp1 participates in Fub functions in cooperation with the architectural proteins Pita and Su(Hw). Thus, Mzfp1 is a new architectural C2H2 protein involved in the organization of active promoters and insulators in Drosophila.
ProtVar: mapping and contextualizing human missense variation
Authors: Stephenson James D , Totoo Prabhat , Burke David F , Jänes Jürgen , Beltrao Pedro , Martin Maria J 
Published: May 2024

Abstract
Genomic variation can impact normal biological function in complex ways and so understanding variant effects requires a broad range of data to be coherently assimilated. Whilst the volume of human variant data and relevant annotations has increased, the corresponding increase in the breadth of participating fields, standards and versioning mean that moving between genomic, coding, protein and structure positions is increasingly complex. In turn this makes investigating variants in diverse formats and assimilating annotations from different resources challenging. ProtVar addresses these issues to facilitate the contextualization and interpretation of human missense variation with unparalleled flexibility and ease of accessibility for use by the broadest range of researchers. By precalculating all possible variants in the human proteome it offers near instantaneous mapping between all relevant data types. It also combines data and analyses from a plethora of resources to bring together genomic, protein sequence and function annotations as well as structural insights and predictions to better understand the likely effect of missense variation in humans. It is offered as an intuitive web server https://www.ebi.ac.uk/protvar where data can be explored and downloaded, and can be accessed programmatically via an API.
Global kidney health priorities—perspectives from the ISN-GKHA
Authors: Okpechi Ikechi G , Luyckx Valerie A , Tungsanga Somkanya , Ghimire Anukul , Jha Vivekanand , Johnson David W , Bello Aminu K 
Published: May 2024

ABSTRACT
Kidney diseases have become a global epidemic with significant public health impact. Chronic kidney disease (CKD) is set to become the fifth largest cause of death by 2040, with major impacts on low-resource countries. This review is based on a recent report of the International Society of Nephrology Global Kidney Health Atlas (ISN-GKHA) which uncovered gaps in key vehicles of kidney care delivery assessed using World Health Organization building blocks for health systems (financing, services delivery, workforce, access to essential medicines, health information systems and leadership/governance). High-income countries had more centres for kidney replacement therapies (KRT), higher KRT access, higher allocation of public funds to KRT, larger workforces, more health information systems, and higher government recognition of CKD and KRT as health priorities than low-income nations. Evidence identified from the current ISN-GKHA initiative should serve as template for generating and advancing policies and partnerships to address the global burden of kidney disease. The results provide opportunities for kidney health policymakers, nephrology leaders and organizations to initiate consultations to identify strategies for improving care delivery and access in equitable, resource-sensitive manners. Policies to increase use of public funding for kidney care, lower the cost of KRT and increase workforces should be a high priority in low-resource nations, while strategies that expand access to kidney care and maintain current status of care should be prioritized in high-income countries. In all countries, the perspectives of people with CKD should be exhaustively explored to identify core kidney care priorities.
Calcineurin inhibitor effects on kidney electrolyte handling and blood pressure: tacrolimus versus voclosporin
Authors: Wei Kuang-Yu , van Heugten Martijn H , van Megen Wouter H , van Veghel Richard , Rehaume Linda M , Cross Jennifer L , Viel John J , van Willigenburg Hester , Silva Pedro Henrique Imenez , Danser A H Jan , de Baaij Jeroen H F , Hoorn Ewout J 
Published: May 2024

ABSTRACT

Background
Calcineurin inhibitors (CNIs) affect kidney electrolyte handling and blood pressure (BP) through an effect on the distal tubule. The second-generation CNI voclosporin causes hypomagnesaemia and hypercalciuria less often than tacrolimus. This suggests different effects on the distal tubule, but this has not yet been investigated experimentally.

Methods
Rats were treated with voclosporin, tacrolimus or vehicle for 28 days. Dosing was based on a pilot experiment to achieve clinically therapeutic concentrations. Drug effects were assessed by electrolyte handling at day 18 and 28, thiazide testing at day 20, telemetric BP recordings and analysis of messenger RNA (mRNA) and protein levels of distal tubular transporters at day 28.

Results
Compared with vehicle, tacrolimus but not voclosporin significantly increased the fractional excretions of calcium (&gt;4-fold), magnesium and chloride (both 1.5-fold) and caused hypomagnesaemia. Tacrolimus but not voclosporin significantly reduced distal tubular transporters at the mRNA and/or protein level, including the sodium–chloride cotransporter, transient receptor melastatin 6, transient receptor potential vanilloid 5, cyclin M2, sodium–calcium exchanger and calbindin-D28K. Tacrolimus but not voclosporin reduced the mRNA level and urinary excretion of epidermal growth factor. The saluretic response to hydrochlorothiazide at day 20 was similar in the voclosporin and vehicle groups, whereas it was lower in the tacrolimus group. The phosphorylated form of the sodium–chloride cotransporter was significantly higher at day 28 in rats treated with voclosporin than in those treated with tacrolimus. Tacrolimus transiently increased BP, whereas voclosporin caused a gradual but persistent increase in BP that was further characterized by high renin, normal aldosterone and low endothelin-1.

Conclusions
In contrast to tacrolimus, voclosporin does not cause hypercalciuria and hypomagnesaemia, but similarly causes hypertension. Our data reveal differences between the distal tubular effects of tacrolimus and voclosporin and provide a pathophysiological basis for the clinically observed differences between the two CNIs.
Empirical and simulation data reveal a lack of avoidance of wind turbines by Gyps fulvus (Griffon Vulture)
Authors: Sassi Yohan , Ziletti Noémie , Duriez Olivier , Robira Benjamin 
Published: May 2024

Abstract
The increase of wind turbine installations to limit climate change may affect bird populations because of collisions with rotor blades. Birds may respond to wind turbine presence along a gradient of behavioral changes: avoiding the wind farm (macro-scale) or the wind turbines either by anticipating wind turbine locations (meso-scale) or engaging in last-minute flee attempts (micro-scale). We investigated flight responses of 25 adult Gyps fulvus (Griffon Vulture) equipped with GPS tags over 3 years in an area that included 10 wind farms in Causses, France. At the macro-scale, foraging range and habitat use revealed that G. fulvus did not avoid wind farms. To investigate avoidance at meso- and micro-scales, we focused on the 4 most visited wind farms. We compared vulture flights to null movement models, which allowed us to keep the correlation between flights and topography while creating movement independent of wind turbine locations. At most sites, G. fulvus did not show avoidance behavior. Overall, our results suggest wind farm-specific responses by soaring birds as a function of landscape topography. Thus, to reduce collision risks, stakeholders should first avoid constructing wind farms close to vulture nesting colonies. When siting, developers should investigate G. fulvus use of local topography to avoid areas in which birds are likely to travel. Finally, managers must prioritize the detection of species not able to avoid turbines when designing preventive measures, such as switching off on-demand technologies.
SEPALLATA-driven MADS transcription factor tetramerization is required for inner whorl floral organ development
Authors: Hugouvieux Veronique , Blanc-Mathieu Romain , Janeau Aline , Paul Michel , Lucas Jeremy , Xu Xiaocai , Ye Hailong , Lai Xuelei , Le Hir Sarah , Guillotin Audrey , Galien Antonin , Yan Wenhao , Nanao Max , Kaufmann Kerstin , Parcy François , Zubieta Chloe 
Published: May 2024

Abstract
MADS transcription factors are master regulators of plant reproduction and flower development. The SEPALLATA (SEP) subfamily of MADS transcription factors is required for the development of floral organs and plays roles in inflorescence architecture and development of the floral meristem. SEPALLATAs act as organizers of MADS complexes, forming both heterodimers and heterotetramers in vitro. To date, the MADS complexes characterized in angiosperm floral organ development contain at least 1 SEPALLATA protein. Whether DNA binding by SEPALLATA-containing dimeric MADS complexes is sufficient for launching floral organ identity programs, however, is not clear as only defects in floral meristem determinacy were observed in tetramerization-impaired SEPALLATA mutant proteins. Here, we used a combination of genome-wide-binding studies, high-resolution structural studies of the SEP3/AGAMOUS (AG) tetramerization domain, structure-based mutagenesis and complementation experiments in Arabidopsis (Arabidopsis thaliana) sep1 sep2 sep3 and sep1 sep2 sep3 ag-4 plants transformed with versions of SEP3 encoding tetramerization mutants. We demonstrate that while SEP3 heterodimers can bind DNA both in vitro and in vivo and recognize the majority of SEP3 wild-type-binding sites genome-wide, tetramerization is required not only for floral meristem determinacy but also for floral organ identity in the second, third, and fourth whorls.
Mussel inspired 3D elastomer enabled rapid calvarial bone regeneration through recruiting more osteoprogenitors from the dura mater
Authors: Wang Xuqiao , Ma Chaoqun , Zhang Xinchi , Yuan Pingping , Wang Yujiao , Fu Mingdi , Zhang Zheqian , Shi Ruiying , Wei Na , Wang Juncheng , Wu Wei 
Published: May 2024

Abstract
Currently, the successful healing of critical-sized calvarial bone defects remains a considerable challenge. The immune response plays a key role in regulating bone regeneration after material grafting. Previous studies mainly focused on the relationship between macrophages and bone marrow mesenchymal stem cells (BMSCs), while dural cells were recently found to play a vital role in the calvarial bone healing. In this study, a series of 3D elastomers with different proportions of polycaprolactone (PCL) and poly(glycerol sebacate) (PGS) were fabricated, which were further supplemented with polydopamine (PDA) coating. The physicochemical properties of the PCL/PGS and PCL/PGS/PDA grafts were measured, and then they were implanted as filling materials for 8 mm calvarial bone defects. The results showed that a matched and effective PDA interface formed on a well-proportioned elastomer, which effectively modulated the polarization of M2 macrophages and promoted the recruitment of dural cells to achieve full-thickness bone repair through both intramembranous and endochondral ossification. Single-cell RNA sequencing analysis revealed the predominance of dural cells during bone healing and their close relationship with macrophages. The findings illustrated that the crosstalk between dural cells and macrophages determined the vertical full-thickness bone repair for the first time, which may be the new target for designing bone grafts for calvarial bone healing.