bluuebunny/crossref_metadata_2025_split huggingface dataset:
Let‐7c functions as a metastasis suppressor by targeting MMP11 and PBX3 in colorectal cancer
Authors: Han Hai‐Bo , Gu Jin , Zuo Hui‐Jing , Chen Zhi‐Guo , Zhao Wei , Li Ming , Ji Deng‐Bo , Lu You‐Yong , Zhang Zhi‐Qian 
Published: October 2011

AbstractAccumulating evidence shows that microRNAs, functioning as either oncogenes or tumour suppressors by negatively regulating downstream target genes that are actively involved in tumour initiation and progression, may be promising biomarkers and therapy targets. Data mining through a microRNA chip database indicated that let‐7c may be associated with tumour metastasis. Here, we confirmed that down‐regulation of let‐7c in primary cancer tissues was significantly associated with metastases, advanced TNM stages and poor survival of colorectal cancer patients. Moreover, ectopic expression of let‐7c in a highly metastatic Lovo cell line remarkably suppressed cell migration and invasion in vitro by the down‐regulation of K‐RAS, MMP11 and PBX3, as well as tumour growth and metastases in vivo, whereas inhibition of let‐7c in low‐metastatic HT29 cells increased cell motility and invasion by the enhanced gene expression of K‐RAS, MMP11 and PBX3. Interestingly, the luciferase reporters' activities with the 3′‐UTRs of K‐RAS, MMP11 and PBX3 were inhibited significantly by let‐7c. Importantly, rescue experiments involving the over‐expression of these genes without their 3′‐UTRs completely reversed the effects of let‐7c on tumour metastasis, both in vitro and in vivo. Finally, the levels of let‐7c were inversely correlated with those of MMP11 and PBX3, but not with those of K‐RAS. Taken together, these results demonstrate that let‐7c, apart from its tumour growth suppression role, also functions as a tumour metastasis suppressor in colorectal cancer by directly destabilizing the mRNAs of MMP11 and PBX3 at least. Copyright © 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Origin of pancreatic ductal adenocarcinoma from atypical flat lesions: a comparative study in transgenic mice and human tissues
Authors: Aichler Michaela , Seiler Christopher , Tost Monica , Siveke Jens , Mazur Pawel K , Da Silva‐Buttkus Patricia , Bartsch Detlef K , Langer Peter , Chiblak Sara , Dürr Anna , Höfler Heinz , Klöppel Günter , Müller‐Decker Karin , Brielmeier Markus , Esposito Irene 
Published: October 2011

AbstractPancreatic ductal adenocarcinoma (PDAC) and its precursor lesions, pancreatic intraepithelial neoplasia (PanIN), display a ductal phenotype. However, there is evidence in genetically defined mouse models for PDAC harbouring a mutated kras under the control of a pancreas‐specific promoter that ductal cancer might arise in the centroacinar‐acinar region, possibly through a process of acinar‐ductal metaplasia (ADM). In order to further elucidate this model of PDAC development, an extensive expression analysis and molecular characterization of the putative and already established (PanIN) precursor lesions were performed in the Kras$^{G{\it{\bf{12}}}D/+}$ ; Ptf1a‐Cre$^{ex{\it{\bf{1}}}/+}$ mouse model and in human tissues, focusing on lineage markers, developmental pathways, cell cycle regulators, apomucins, and stromal activation markers. The results of this study show that areas of ADM are very frequent in the murine and human pancreas and represent regions of increased proliferation of cells with precursor potential. Moreover, atypical flat lesions originating in areas of ADM are the most probable precursors of PDAC in the Kras$^{G{\it{\bf{12}}}D/+}$; Ptf1a‐Cre$^{ex{\it{\bf{1}}}/+}$ mice and similar lesions were also found in the pancreas of three patients with a strong family history of PDAC. In conclusion, PDAC development in Kras$^{G{\it{\bf{12}}}D/+}$; Ptf1a‐Cre$^{ex{\it{\bf{1}}}/+}$ mice starts from ADM and a similar process might also take place in patients with a strong family history of PDAC. Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Aniline oligomers versus polyaniline
Authors: Stejskal Jaroslav , Trchová Miroslava 
Published: October 2011

AbstractThis paper deals with aniline oligomers produced by the oxidation of aniline with ammonium peroxydisulfate under alkaline conditions. Oligomers obtained in 1 mol L−1 ammonium hydroxide as microspheres have been analyzed. They are brown, non‐conducting, and are composed of tens of aniline constitutional units. They contain oxygen in semiquinonoid and quinonoid constitutional units or both, and sulfur in sulfonic or sulfate groups. Phenazine‐like units in the oligomers have been identified by UV‐visible spectra and the IR spectra support them. IR spectroscopic analysis of fractions insoluble and soluble in chloroform suggests that aniline oligomers are not uniform and strong hydrogen bonding occurs in their structure. Aniline oligomers will be potentially useful in applications that do not require conductivity but use the redox or salt–base transitions typical of polyaniline. Their applicability is illustrated by the ability to reduce silver cations to silver, base–salt transitions and in the carbonization to nitrogen‐containing carbons. Copyright © 2011 Society of Chemical Industry
Melanoma survivors: health behaviors, surveillance, psychosocial factors, and family concerns
Authors: Oliveria Susan A. , Shuk Elyse , Hay Jennifer L. , Heneghan Maureen , Goulart Jacqueline M. , Panageas Katherine , Geller Alan C. , Halpern Allan C. 
Published: October 2011

AbstractBackgroundLittle research has been conducted on melanoma survivors and important opportunities exist for research in this understudied population. The study objective was to examine experiences of melanoma survivors regarding sun protection, surveillance practices, psychosocial and family concerns using focus groups.MethodsWe identified patients (≥18 years) treated at Memorial Sloan‐Kettering Cancer Center (USA) during 1996–2005 with invasive primary cutaneous melanoma, stages I–III. We stratified focus groups by age (<50 vs ≥50 years) and years since diagnosis (1 to <5 years vs 5–10 years). We performed qualitative thematic text analysis, which involved independent data review by multiple analysts (n = 2) followed by interpretation of key findings via consensus work.ResultsForty‐eight survivors composed the final sample. Most did not conduct routine skin self‐examinations. Survivors became more conscious of sun exposure and expanded their sun protection; some experienced anxiety if caught unprotected in the sun. Survivors were aware of the increased melanoma risk for family members, yet many did not discuss risk reduction. Melanoma diagnosis elevated the importance of retaining health insurance and purchasing life insurance for younger survivors. We did not identify significance difference between those diagnosed more or less recently.ConclusionsWe identified areas of high priority to melanoma survivors: skin self‐examination, anxiety associated with sun exposure, familial risk communication, and maintaining health insurance benefits. The implications for cancer survivors are that these behavioral data will help to design health promotion and prevention programs that are tailored to melanoma survivors' risk status. Copyright © 2011 John Wiley & Sons, Ltd.
Plant volatile compounds shorten reaction time and enhance attraction of the codling moth (Cydia pomonella) to codlemone
Authors: Schmera Dénes , Guerin Patrick M 
Published: October 2011

AbstractBACKGROUND: The codling moth is the most serious pest of deciduous tree fruit (apples, pears, crabapples, walnuts, quince) worldwide. The high frequency of insecticide treatments per season has resulted in breakdown of codling moth control owing to insecticide resistance. As an alternative, integrated pest management includes mating disruption to achieve population suppression in orchards. Under this scheme, the sex pheromone of the codling moth, (E, E)‐8,10‐dodecadien‐1‐ol (codlemone), is released from dispensers in crops to hinder mating by luring males. Increasing the attractiveness of codlemone formulations to codling moth males can be regarded as a key to increasing the efficacy of mating disruption. With this aim, the effects of adding plant volatiles on the behavioural responses of codling moth males to codlemone were tested.RESULTS: Adding R(+)‐limonene, linalool, (E)‐β‐farnesene or ethyl (E, Z)‐2,4‐decadienoate to codlemone significantly increases the proportion of males flying to the pheromone source in a wind tunnel. The response level is equivalent to that of males responding to females releasing codlemone. Using real‐time recordings, it is shown how these four plant products also shorten the response time of males to codlemone under the behavioural criteria time to activation, time till upwind flight is induced and time to pheromone source contact.CONCLUSION: Shortening the response time and increasing source location by males of dispensers releasing codlemone with R(+)‐limonene, linalool, (E)‐β‐farnesene or ethyl (E, Z)‐2,4‐decadienoate added would enhance mating disruption through better engagement of males with dispensers, to the detriment of females. Copyright © 2011 Society of Chemical Industry
Use of a potentiometric vital dye to determine the effect of the herbicide bromoxynil octanoate on mitochondrial bioenenergetics in Chlamydomonas reinhardtii
Authors: Brickley Mark R , Lawrie Emily , Weise Veronika , Hawes Chris , Cobb Andrew H 
Published: October 2011

AbstractBACKGROUND: The aims of the present study were to validate a vital mitochondrial potentiometric staining method in Chlamydomonas reinhardtii and to utilise this method to examine the effect of the herbicide bromoxynil octanoate on mitochondrial potential in this species. A range of stains was investigated, including Rhodamine 123, DASPMI, Mitotracker Green, Mitotracker Orange and JC‐1.RESULTS: Rhodamine 123 (R123) had the highest utility of several candidate stains. Incubation with both 5 and 10 µM carbonyl cyanide 3‐chlorophenylhydrazone caused significant fluorescence collapse [Dunn's post test (40.00, P < 0.01) and (45.49, P < 0.01) respectively], demonstrating that the R123 fluorescence reported mitochondrial potential. The effect of the herbicide bromoxynil octanoate was examined. Exposure to 0.1 mM of bromoxynil resulted in a significant increased mitochondrial fluorescence compared with the baseline (Mann–Whitney U = 222, P < 0.002), while concentrations of 1 mM and greater resulted in significant, almost complete loss of mitochondrial potential [mean fluorescence ratio = 1.193–1.289 (where a ratio of 1 represents total potential loss), Mann–Whitney U = 0.0, P < 0.001 (1 mM), 0.0, P < 0.0001 (2 mM), 0.0, P < 0.0001 (5 mM)]. EC50 of the collapse in mitochondrial potential owing to bromoxynil incubation occurred at 0.72 mM, and the mean t50 of bromoxynil octanoate action was 93 s.CONCLUSIONS: R123 is a sensitive potentiometric dye in C. reinhardtii that may find further use in investigations of both mitochondrial bioenergetics in plants and environmental toxicology. Copyright © 2011 Society of Chemical Industry
Validation of RNA interference in western corn rootworm Diabrotica virgifera virgifera LeConte (Coleoptera: Chrysomelidae) adults
Authors: Rangasamy Murugesan , Siegfried Blair D 
Published: October 2011

AbstractBACKGROUND: RNA interference (RNAi) is commonly used in insect functional genomics studies and usually involves direct injection of double‐stranded RNA (dsRNA). Only a few studies have involved exposure to dsRNAs through feeding. For western corn rootworm (Diabrotica virgifera virgifera) larvae, ingestion of dsRNA designed from the housekeeping gene, vacuolar ATPase (vATPase) triggers RNAi causing growth inhibition and mortality; however, the effect of dsRNA feeding on adults has not been examined. In this research, WCR adults were fed with vATPase‐dsRNA‐treated artificial diet containing a cucurbitacin bait, which is a proven feeding stimulant for chrysomelid beetles of the subtribe Diabroticina to which rootworms belong.RESULTS: Real‐time PCR confirmed suppression of vATPase expression and western blot analysis indicated reduced signal of a protein that cross‐reacted with a vATPase polyclonal antiserum in WCR adults exposed to artificial diet treated with dsRNA and cucurbitacin bait. Continuous feeding on cucurbitacin and dsRNA‐treated artificial diet resulted in more than 95% adult mortality within 2 weeks while mortality in control treatments never exceeded 20%.CONCLUSIONS: This research clearly demonstrates the effect of RNAi on WCR adults that have been exposed to dsRNA by feeding and establishes a tool to screen dsRNAs of potential target genes in adults. This technique may serve as an alternative to target screening of larvae which are difficult to maintain on artificial diets. Copyright © 2011 Society of Chemical Industry
Evaluation of the factors affecting the optimal fiducial configurations calculated through a genetic‐algorithm‐based methodology in image‐guided neurosurgery
Authors: Battezzato Alessandro , Gastaldi Laura , Pastorelli Stefano 
Published: October 2011

AbstractBackgroundImage‐guided neurosurgery usually involves a point‐pair registration between two spaces, associating the patient in the operating room with pre‐operative image scans. The distribution and number of fiducial markers during registration are critical for the expected error at the target point.MethodsA genetic algorithm has been designed to provide an optimal marker configuration. The solution, visualized on a 3D head reconstruction, is intended as a guideline for the surgeon to properly place the markers. However, deviations from ideal configurations occur during marker placement; moreover, the actual target is not a point, but a region. The consequent decrease of target accuracy is statistically investigated.ResultsA requirement on target minimum accuracy can be satisfied in the operating room not only by setting the number of markers for the optimized fiducial configuration, but also by considering the inevitable sources of error.ConclusionsQuantifying the sources of error that affect a genetic‐algorithm‐based optimization shows that it is still convenient. Target point correct individuation is particularly important, as it strongly influences the optimization performance. Copyright © 2011 John Wiley & Sons, Ltd.
Preliminary application of computer‐assisted patient‐specific acetabular navigational template for total hip arthroplasty in adult single development dysplasia of the hip
Authors: Zhang Yuan Z. , Chen Bin , Lu Sheng , Yang Yong , Zhao Jian M. , Liu Rui , Li Yan B. , Pei Guo X. 
Published: October 2011

AbstractBackgroundThe considerable variation in anatomical abnormalities of hip joints associated with different types of developmental dysplasia of hip (DDH) makes reconstruction in total hip arthroplasty (THA) difficult. It is desirable to create patient‐specific designs for THA procedures. In the cases of adult single DDH, an accuracy‐improved method has been developed for acetabular cup prosthesis implantation of hip arthroplasty.MethodsFrom October 2007 to November 2008, 22 patients with single DDH (according to the Crowe standard, all dysplasia hips were classified as type I) were scanned with spiral CT pre‐operatively. These patients scheduled for THA were randomly assigned to undergo either conventional THA (control group, n = 11) or navigation template implantation (NT group, n = 11). In the NT group, three‐dimensional (3D) CT pelvis image data were transferred to a computer workstation and 3D models of the hip were reconstructed using the Mimics software. The 3D models were then processed by the Imageware software. In brief, a template that best fitted the location and shape of the acetabular cup was ‘reversely’ built from the 3D model, the rotation centre of the pathological hip determined by mirroring that of the healthy site, and a guiding hole in the template was then designed. The navigational templates were manufactured using a rapid prototyping machine. These navigation templates guide acetabular component placement.ResultsBased on the predetermined abduction angle 45° and anteversion angle 18°, after 1 year follow‐up, the NT group showed significantly smaller differences (1.6° ± 0.4°, 1.9° ± 1.1°) from the predetermined angles than those in the control group (5.8° ± 2.9°, 3.9° ± 2.5°) (P < 0.05).ConclusionsThe template designs facilitated accurate placement of acetabular components in dysplasia of acetabulum. The hip's center of rotation in DDH could be established using computer‐aided design, which provides a useful method for the accurate location of prosthesis with a low cost–performance ratio without excessive technical workload on the surgical team. Copyright © 2011 John Wiley & Sons, Ltd.
Robotic presacral neurectomy – technique and results
Authors: Kapetanakis Vasilis , Jacob Kristina , Klauschie Jennifer , Kho Rosanne , Magrina Javier 
Published: October 2011

AbstractBackgroundThe feasibility, safety and outcomes of robotic presacral neurectomy were evaluated.MethodsA retrospective analysis was performed of 33 patients with midline chronic pelvic pain, who underwent robotic presacral neurectomy. Data were collected during the period March 2004–March 2010.ResultsAll patients underwent robotic presacral neurectomy and additional robotic procedures. Mean operating time for robotic presacral neurectomy was 5.8 (range 4.4–6.9) min. Mean blood loss, including concomitant procedures, was 39 (range 15–150) ml. Most patients were discharged the same day (60%) and mean hospital stay was 0.39 (range 0–2) days. There were no conversions or intra‐operative complications. There were two postoperative complications: one pelvic abscess and one Clostridium difficile colitis. Pain improvement was reported by 73% (22/30) of patients.ConclusionRobotic presacral neurectomy is safe and feasible, resulting in improvement of pelvic pain in selected patients. Copyright © 2011 John Wiley & Sons, Ltd.
Design of an integrated master–slave robotic system for minimally invasive surgery
Authors: Li Jianmin , Zhou Ningxin , Wang Shuxin , Gao Yuanqian , Liu Dongchun 
Published: October 2011

AbstractBackgroundMinimally invasive surgery (MIS) robots are commonly used in hospitals and medical centres. However, currently available robotic systems are very complicated and huge, greatly raising system costs and the requirements of operating rooms. These disadvantages have become the major impediments to the expansion of MIS robots.MethodsAn integrated MIS robotic system is proposed based on the analysis of advantages and disadvantages of different MIS robots. In the proposed system, the master manipulators, slave manipulators, image display device and control system have been designed as a whole. Modular design is adopted for the control system for easy maintenance and upgrade. The kinematic relations between the master and the slave are also investigated and embedded in software to realize intuitive movements of hand and instrument. Finally, animal experiments were designed to test the effectiveness of the robot.ResultsThe robot realizes natural hand–eye movements between the master and the slave to facilitate MIS operations. The experimental results show that the robot can realize similar functions to those of current commercialized robots.ConclusionsThe integrated design simplifies the robotic system and facilitates use of the robot. Compared with the commercialized robots, the proposed MIS robot achieves similar functions and features but with a smaller size and less weight. Copyright © 2011 John Wiley & Sons, Ltd.
Local Anesthesia: A Strategy for Reducing Surgical Site Infections?
Authors: Lee Jay S. , Hayanga Awori J. , Kubus James J. , Makepeace Henry , Hutton Max , Campbell Darrell A. , Englesbe Michael J. 
Published: October 2011

AbstractBackgroundSurgical site infections (SSIs) contribute to increased morbidity, mortality, and hospitalization costs. A previously unidentified factor that may reduce SSIs is the use of local anesthesia. The objective of this study was to determine if the use of local anesthesia is independently associated with a lower incidence of SSIs compared to nonlocal anesthesia.MethodsUsing the American College of Surgeons National Surgical Quality Improvement Program database (2005–2007), we identified all patients undergoing surgical procedures that could be performed using local or general anesthesia, depending on the preference of the surgeon. Logistic regression was used to identify factors independently associated with the use of local anesthesia. Propensity matching was then used to match local and nonlocal anesthesia cases while controlling for patient and operative characteristics. SSI rates were compared using a χ2 test.ResultsOf 111,683 patients, 1928 underwent local anesthesia; and in 109,755 cases the patients were given general anesthesia where a local anesthetic potentially could have used. In the unmatched analysis, patients with local anesthesia had a significantly lower incidence of SSIs than patients with nonlocal anesthesia (0.7 vs. 1.4%, P = 0.013). Similarly, after propensity matching, the incidence of SSIs in patients given local anesthesia was significantly lower than for that of patients given nonlocal anesthesia (0.8 vs. 1.4%, P = 0.043).ConclusionsUse of local anesthesia is independently associated with a lower incidence of SSIs. It may provide a safe, simple approach to reducing the number of SSIs.
Phase I trial of preoperative intratumoral injection of immature dendritic cells and OK‐432 for resectable pancreatic cancer patients
Authors: Endo Hisahito , Saito Takuro , Kenjo Akira , Hoshino Mika , Terashima Masanori , Sato Tetsu , Anazawa Takayuki , Kimura Takashi , Tsuchiya Takao , Irisawa Atsushi , Ohira Hiromasa , Hikichi Takuto , Takagi Tadayuki , Gotoh Mitsukazu 
Published: October 2011

AbstractPurposeTo determine the feasibility, safety and histological change of preoperative endoscopic ultrasound‐guided fine‐needle injection (PEU‐FNI) of immature DCs (iDCs) with OK‐432 in pancreatic cancer patients.MethodsNine patients enrolled in the trial (DC group) and were compared with 15 patients operated on without iDC injection (non‐DC group). Adverse events of PEU‐FNI and postoperative complications were evaluated according to CTC‐AE ver.3.0 and the Clavien—Dindo classification/ISGPF definition, respectively. Histological changes within the tumor and lymph nodes were evaluated by immunohistochemical examination of infiltrating inflammatory cells (CD4+, CD8+, Foxp3+ and CD83+).ResultsThere were no severe toxicities following PEU‐FNI, except for one transient grade 3 fever, and there were no significant differences in the incidence of postoperative complications between the two groups. Colliquative necrosis and diffusely scattered TUNEL‐positive cells were observed at the injection sites. CD83+ cells significantly accumulated in the regional lymph nodes of the DC group as well as Foxp3+ cells in the regional and distant lymph nodes. The two DC group patients, one of which was stage IV with distant lymph node metastasis, survived more than 5 years without requiring adjuvant theraphy.ConclusionPEU‐FNI was feasible and safe, and further study needs to confirm and enhance antitumor responses.