bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interpolation among reduced‐order matrices to obtain parameterized models for design, optimization and probabilistic analysis
Authors: Degroote Joris , Vierendeels Jan , Willcox Karen 
Published: June 2009

AbstractModel reduction has significant potential in design, optimization and probabilistic analysis applications, but including the parameter dependence in the reduced‐order model (ROM) remains challenging. In this work, interpolation among reduced‐order matrices is proposed as a means to obtain parameterized ROMs. These ROMs are fast to evaluate and solve, and can be constructed without reference to the original full‐order model. Spline interpolation of the reduced‐order system matrices in the original space and in the space tangent to the Riemannian manifold is compared with Kriging interpolation of the predicted outputs. A heuristic criterion to select the most appropriate interpolation space is proposed. The interpolation approach is applied to a steady‐state thermal design problem and probabilistic analysis via Monte Carlo simulation of an unsteady contaminant transport problem. Copyright © 2009 John Wiley & Sons, Ltd.
Relation between brain lesion location and clinical outcome in patients with severe traumatic brain injury: A diffusion tensor imaging study using voxel‐based approaches
Authors: Perlbarg Vincent , Puybasset Louis , Tollard Eléonore , Lehéricy Stéphane , Benali Habib , Galanaud Damien 
Published: June 2009

AbstractThe early prediction of consciousness recovery from traumatic brain injury (TBI) is crucial to make decisions about the appropriate use of prolonged intensive care. Diffusion tensor imaging (DTI) has been proposed as a biomarker of white matter injury that could be used in a classification purpose. Instead of region‐of‐interest‐based approach, we applied voxel‐based approaches (voxel‐based DTI and tract‐based spatial statistics) on 30 patients with TBI to identify, without any prior, the brain regions that were specifically damaged in unfavorable 1‐year outcome group compared to the favorable one. DTI were acquired at mean 23 days (5–53 days) and two DTI‐derived indices, fractional anisotropy (FA) and apparent diffusion coefficient (ADC), were tested. Our results showed that (1) ADC is not a relevant biomarker for early 1‐year outcome prognosis; (2) FA measured in inferior longitudinal fasciculus, in cerebral peduncle, in posterior limb of the internal capsule, and in posterior corpus callosum is specifically decreased in unfavorable outcome group compare to the favorable one; (3) a linear discriminant analysis using the FA measured in these four regions showed good classification performance (sensitivity = 86% and specificity = 86%). These findings confirm the relevance of the use of DTI as biomarkers for consciousness recovery after TBI and support the possible use of these biomarkers for early classification of patients. Hum Brain Mapp, 2009. © 2009 Wiley‐Liss, Inc.
Entorhinal cortex structure and functional MRI response during an associative verbal memory task
Authors: Braskie Meredith N. , Small Gary W. , Bookheimer Susan Y. 
Published: June 2009

AbstractEntorhinal cortex (ERC) volume in adults with mild cognitive impairment has been shown to predict prodromal Alzheimer's disease (AD). Likewise, neuronal loss in ERC has been associated with AD, but not with normal aging. Because ERC is part of a major pathway modulating input to the hippocampus, structural changes there may result in changes to cognitive performance and functional brain activity during memory tasks. In 32 cognitively intact older adults, we examined the relationship between left ERC thickness and functional magnetic resonance imaging (fMRI) activity during an associative verbal memory task. This task has been shown previously to activate regions that are sensitive to aging and AD risk. ERC was manually defined on native space, high resolution, oblique coronal MRI scans. Subjects having thicker left ERC showed greater activation in anterior cingulate and medial frontal regions during memory retrieval, but not encoding. This result was independent of hippocampal volume. Anterior cingulate cortex is directly connected to ERC, and is, along with medial frontal cortex, implicated in error detection, which is impaired in AD. Our results suggest that in healthy older adults, processes that engage frontal regions during memory retrieval are related to ERC structure. Hum Brain Mapp, 2009. © 2009 Wiley‐Liss, Inc.
Noninvasive dynamic imaging of seizures in epileptic patients
Authors: Tyvaert Louise , LeVan Pierre , Dubeau Francois , Gotman Jean 
Published: June 2009

AbstractEpileptic seizures are due to abnormal synchronized neuronal discharges. Techniques measuring electrical changes are commonly used to analyze seizures. Neuronal activity can be also defined by concomitant hemodynamic and metabolic changes. Simultaneous electroencephalogram (EEG)‐functional MRI (fMRI) measures noninvasively with a high‐spatial resolution BOLD changes during seizures in the whole brain. Until now, only a static image representing the whole seizure was provided. We report in 10 focal epilepsy patients a new approach to dynamic imaging of seizures including the BOLD time course of seizures and the identification of brain structures involved in seizure onset and discharge propagation. The first activation was observed in agreement with the expected location of the focus based on clinical and EEG data (three intracranial recordings), thus providing validity to this approach. The BOLD signal preceded ictal EEG changes in two cases. EEG‐fMRI may detect changes in smaller and deeper structures than scalp EEG, which can only record activity form superficial cortical areas. This method allowed us to demonstrate that seizure onset zone was limited to one structure, thus supporting the concept of epileptic focus, but that a complex neuronal network was involved during propagation. Deactivations were also found during seizures, usually appearing after the first activation in areas close or distant to the activated regions. Deactivations may correspond to actively inhibited regions or to functional disconnection from normally active regions. This new noninvasive approach should open the study of seizure generation and propagation mechanisms in the whole brain to groups of patients with focal epilepsies. Hum Brain Mapp, 2009. © 2009 Wiley‐Liss, Inc.
N → C3 migration of dichlorophosphino group in the phosphorylation of N‐unsubstituted indole with phosphorus trichloride
Authors: Chaikovskaya Aleksandra A. , Dmytriv Yurii V. , Shevchuk Nadiya V. , Smaliy Radomyr V. , Pinchuk Aleksander M. , Tolmachev Andrey A. 
Published: June 2009

AbstractA N → C3 migration of the dichlorophosphino group has been revealed in the phosphorylation of unsubstituted indole with phosphorus trichloride. Several 3‐phosphorylated NH‐indole derivatives have thus been obtained and N‐acylation of one of the products has been performed. © 2009 Wiley Periodicals, Inc. Heteroatom Chem 20:235–239, 2009; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/hc.20540
Restriction of cisplatin induction of acute apoptosis to a subpopulation of cells in a three‐dimensional carcinoma culture model
Authors: Fayad Walid , Brnjic Slavica , Berglind Daniel , Blixt Susanna , Shoshan Maria C. , Berndtsson Maria , Olofsson Maria Hägg , Linder Stig 
Published: June 2009

AbstractCisplatin is a clinically important chemotherapeutical agent used to treat epithelial malignancies. High concentrations (20–100 μM) of cisplatin have been used in numerous studies to induce apoptosis of carcinoma cells grown in monolayer culture over 24–48 hr. These conditions may not be relevant to 3‐D tumor tissue in vivo and the importance of apoptosis for tumor response is controversial. We here studied the effects of cisplatin on a 3‐D colon carcinoma in vitro model (multicellular spheroids). Cisplatin at a dose of 40 μM induced active caspase‐3 preferentially in the peripheral 30 μm cell layer of spheroids, mainly during late stages (72–96 hr). The p53 response to cisplatin was also largely confined to peripheral cell layers. Despite the use of a high cisplatin concentration, a significant fraction of the cells in the spheroids survived treatment. A high proportion of surviving cells stained positive for β‐galactosidase, a marker of premature senescence. Cells growth‐arrested by cisplatin treatment showed a higher spontaneous cell death rate than untreated proliferating cells. We propose that acute apoptosis is of minor significance for the overall response of carcinoma cells to cisplatin treatment. © 2009 UICC
Fisetin, a natural flavonoid, targets chemoresistant human pancreatic cancer AsPC‐1 cells through DR3‐mediated inhibition of NF‐κB
Authors: Murtaza Imtiyaz , Adhami Vaqar Mustafa , Hafeez Bilal Bin , Saleem Mohammad , Mukhtar Hasan 
Published: June 2009

AbstractDeath receptors of the tumor necrosis factor (TNF) receptor super family have been implicated in constitutive activation of nuclear factor‐kappa B (NF‐κB) in pancreatic cancer (PaC) cells. In this study, we demonstrate that fisetin, a natural flavonoid, induces apoptosis and inhibits invasion of chemoresistant PaC AsPC‐1 cells through suppression of DR3‐mediated NF‐κB activation. Fisetin treatment resulted in dose‐dependent inhibition of PaC cell growth and cell proliferation with concomitant induction of apoptosis. A cDNA array analysis revealed that fisetin modulates expression of more than 20 genes at transcription level with maximum decrease observed in DR3 expression and a parallel increase observed in the expression levels of IκBα, an NF‐κB inhibitor. Down‐regulation of DR3 in PaC cells was found to down regulate activated pNF‐κB/p65, pIkBα/β kinases (pIKK's), MMP9 and XIAP that mostly impart chemoresistance in PaC. Immunoblotting and EMSA analysis showed a marked decrease in pNF‐κB and NF‐κB DNA binding activity, respectively, with modest decrease in NF‐κB promoter activity and significant decrease in MMP9 promoter activity with fisetin treatment. Importantly, consistent with these findings, we further found that transient down‐regulation of DR3 by RNA interference significantly augmented fisetin induced changes in cell proliferation, cell invasion and apoptosis paralleled with decrease in pNF‐κB, pIKKα/β, MMP9, XIAP and NF‐κB DNA binding activity. Blocking of DR3 receptor with an extra cellular domain blocking antibody demonstrated similar effects. These data provide evidence that fisetin could provide a biological rationale for treatment of pancreatic cancer or as an adjuvant with conventional therapeutic regimens. © 2009 UICC
The shared tumor associated antigen cyclin‐A2 is recognized by high‐avidity T‐cells
Authors: Kondo Eisei , Maecker Britta , Draube Andreas , Klein‐Gonzalez Nela , Shimabukuro‐Vornhagen Alexander , Schultze Joachim L. , von Bergwelt‐Baildon Michael S. 
Published: June 2009

AbstractCyclin‐A2, a key cell cycle regulator, has been shown to be overexpressed in various types of malignancies with little expression in normal tissue. Such tumor‐associated genes potentially are useful targets for cancer immunotherapy. However, high‐avidity cyclin‐specific T cells are considered to be thymically deleted. We identified at least one nonameric HLA‐A*0201 binding cyclin‐A2 epitope by a reverse immunology approach. Using a highly efficient T‐cell expansion system that is based on CD40‐activated B (CD40‐B) cells as sole antigen‐presenting cells we successfully generated cyclin‐A2 specific CTL from HLA‐A*0201+ donors. Interestingly, high‐avidity cyclin‐A2 specific CTL lines, which recognized peptide‐pulsed and antigen expressing target cells, were indeed generated by stimulation with CD40‐B cells when pulsed with low concentrations of peptide, whereas CD40‐B cells pulsed at saturating concentrations could only induce low‐avidity CTL, which recognized peptide‐pulsed target cells only. One high‐avidity CTL line was subcloned and CTL clones, whose peptide concentration required for half‐maximal lysis were less than 1 nM, could lyse cyclin‐A2 expressing tumor cells. Taken together, cyclin A2 is an attractive candidate for immune intervention in a significant number of cancer patients and high‐avidity T cells can be readily generated using CD40‐B cells as antigen‐presenting cells. © 2009 UICC
Temporal trends over 3 decades and intrafamilial clustering of HPV types in Swedish patients with cervical cancer in situ
Authors: Ivansson Emma L. , Gustavsson Inger M. , Wilander Erik , Magnusson Patrik K.E. , Gyllensten Ulf B. 
Published: June 2009

AbstractInformation on HPV type distribution in cervical cancer in situ in different populations is needed for evaluation of prophylactic vaccination programs targeting HPV 16 and 18. In our study, the HPV type prevalence in 1,079 Swedish women from multicase families diagnosed with cervical cancer in situ 1965–1993 was investigated using real‐time PCR and archival tissue material. HPV type information was obtained for 974 samples. Among these, HPV 16 (61%) was the dominant type followed by HPV 33/52/58 (24%), HPV 31 (13%) and HPV 18/45 (12%). The detected prevalence of HPV 16 among cancer in situ decreased by 13% over the study period while the group of low frequency high‐risk types increased. Related women were not prone to infection by the same type. These data suggest that the prevalence of individual HPV types has changed over time in Swedish patients with cervical cancer in situ. Large‐scale studies of pathology biobank materials will enable further insight into the temporal changes of individual HPV types, as baseline information to properly evaluate the effect of vaccine programs. The findings also indicate that genetic susceptibility to cervical cancer operates through general and not type specific susceptibility to HPV infection. © 2009 UICC
Strategies for digital mammography interpretation in a clinical patient population
Authors: van den Biggelaar Frank J.H.M. , Kessels Alphons G.H. , van Engelshoven Jos M.A. , Flobbe Karin 
Published: June 2009

AbstractMammography is the basic imaging modality for early detection of breast cancer. The aim of this prospective study was to evaluate the impact of different mammogram reading strategies on the diagnostic yield in a consecutive patient population referred for digital mammography to a hospital. First, the effect of using computer‐aided detection (CAD) software on the performance of mammogram readers was studied. Furthermore, the impact of employing technologists as either prereaders or double readers was assessed, as compared to the conventional strategy of single reading by a radiologist. Digital mammograms of 1,048 consecutive patients were evaluated by a radiologist and 3 technologists with and without the use of CAD software. ROC analysis was used to study the effects of the different strategies. In the conventional strategy, an overall area under the curve (AUC) of 0.92 was found, corresponding to a sensitivity of 84% and specificity of 94%. When applying CAD software, the AUCs were similar before and after CAD for all readers (mean of 0.95). Employing technologists in prereading and double reading of mammograms resulted in a mean AUC of 0.91 and 0.96, respectively. In the prereading strategy, the corresponding sensitivity and specificity were 81 and 96%; in the double reading strategy they were 96 and 79%, respectively. Concluding, in this clinical population, systematic application of CAD software by either radiologist or technologists failed to improve the diagnostic yield. Furthermore, employing technologists as double readers of mammograms was the most effective strategy in improving breast cancer detection in daily clinical practice. © 2009 UICC
Morphological studies on machined implants of commercially pure titanium and titanium alloy (Ti6Al4V) in the rabbit
Authors: Palmquist Anders , Lindberg Fredrik , Emanuelsson Lena , Brånemark Rickard , Engqvist Håkan , Thomsen Peter 
Published: June 2009

AbstractThe aim of this study was to evaluate the bone response to commercially pure titanium grade I and titanium alloy grade V (90% Ti, 6% Al, and 4% V, depicted Ti6Al4V) after 8 weeks in rabbit tibia. Interference microscopy and scanning electron microscopy were used for surface analyses. Transmission electron microscopy (TEM) was used for evaluation of surface crystallinity and chemistry after preparation of ultrathin sections using focused ion beam (FIB) microscopy. Three different embedding resins commonly used for histological preparation were evaluated with respect to adaptation to a turned implant surface. Epoxy Agar 100 resin and acrylic Technovit 7200 resin showed low separation while acrylic LR White resin showed large separation at the interface. The retrieved specimens were embedded in acrylic Technovit 7200 resin after fixation and dehydration. The histological evaluation revealed osseointegration for both c.p. titanium grade I and Ti6Al4V alloy, but no quantitative differences in bone contact and bone area were detected. Because a separation of implant and tissue occurred in the interface between implant and bone embedded in acrylic Technovit 7200 resin, additional factors related to implant surface properties and technical procedures are likely to influence the possibilities to prepare ultrathin sections by FIB. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
Favorable effects of the detergent and enzyme extraction method for preparing decellularized bovine pericardium scaffold for tissue engineered heart valves
Authors: Yang Min , Chen Chang‐Zhi , Wang Xue‐Ning , Zhu Ya‐Bin , Gu Y. John 
Published: June 2009

AbstractBovine pericardium has been extensively applied as the biomaterial for artificial heart valves and may potentially be used as a scaffold for tissue‐engineered heart valves after decellularization. Although various methods of decellularization are currently available, it is unknown which method is the most ideal one for the decellularization for bovine pericardium. We compared three decellularization methods, namely, the detergent and enzyme extraction (DEE), the trypsin (TS), and the Triton X‐100 and sodium‐deoxycholate (TSD) method, to examine their efficacy on cell removal and their preservation of the mechanical function and the tissue matrix structure. Results indicated that decellularization was achieved by all the three methods as confirmed by hematoxylin‐eosin staining, scanning electron microscopy, as well as quantitative DNA measurement. However, TS and TSD methods resulted in severe structural destruction of the bovine pericardium as shown by von Gieson staining and Gomori staining. Furthermore, both TS and TSD methods changed the mechanical property of the bovine pericardium, as evidenced by a lower elastic modulus, maximal‐stress, maximal‐disfiguration, maximal‐load, and maximal‐strain. In conclusion, the DEE method achieved both a complete decellularization and preservation of the mechanical function and tissue structure of the bovine pericardium. Thus, this method is superior to either the TS or the TSD method for preparing decellularized bovine pericardium scaffold for constructing tissue‐engineered heart valves. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
The use of collagen cross‐linking agents to enhance dentin bond strength
Authors: Al‐Ammar Aiman , Drummond James L. , Bedran‐Russo Ana Karina 
Published: June 2009

AbstractType I collagen is a major component of the hybrid layer, and improvement of its mechanical properties may be advantageous during bonding procedures.Objective:To investigate the effect of three different cross‐linking agents (Glutaraldehyde [GD], Grape seed extract [GSE], and Genipin [GE]) on the tensile bond strength (TBS) of resin‐dentin bonds.Materials and Methods:Sixty‐four sound human molars were collected and their occlusal surfaces were ground flat to expose dentin. Dentin surfaces were etched using a phosphoric acid and then teeth were randomly divided according to the dentin treatment: Control group (no treatment), 5% GD, 6.5% GSE, or 0.5% GE. Teeth were restored either with One Step Plus or Adper Single Bond Plus adhesive systems and resin composite. After 24 h, teeth were sectioned to produce a cross‐sectional surface area of 1.0 mm2 and tested for tensile bond strength. Data were statistically analyzed using ANOVA and Fisher's PLSD tests (p < 0.05). There was a statistically significant interaction between factors (treatment and adhesive p < 0.001). Treatment affected TBS (p < 0.0001), while no differences were observed between the adhesive systems (p = 0.6961).Conclusion:Chemical modification to the dentin matrix promoted by GD and GSE, but not GE, resulted in increased bond strength. The application of selective collagen cross‐linkers during adhesive restorative procedures may be a new approach to improve dentin bond strength properties. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
Development of β‐tricalcium phosphate/sol‐gel derived bioactive glass composites: Physical, mechanical, and in vitro biological evaluations
Authors: Hesaraki Saeed , Safari Mojgan , Shokrgozar Mohammad Ali 
Published: June 2009

AbstractIn this study, composites of β‐tricalcium phosphate (β‐TCP) and sol gel derived bioactive glass (10, 25, and 40 wt %) based on the SiO2‐CaO‐MgO‐P2O5 system were prepared and sintered at 1000–1200°C. The mechanical properties were investigated by measuring bending strength, Vickers hardness and fracture toughness. Structural properties were evaluated by XRD and SEM analysis, and the biological properties were studied by soaking the samples in simulated body fluid (SBF) and in contact with osteoblastic cell for viability assay. When the samples were sintered at 1200°C, the mechanical strength increased, up to 34%, by increasing the amount of bioactive glass phase. In contrast, it decreased when the samples were sintered at 1000 and 1100°C. The results showed that the strength could be improved up to 56% when more firing period was used. Incorporation of the bioactive glass phase into β‐TCP increased the microhardness but did not significantly change the fracture toughness. Phase analysis revealed that β‐TCP or magnesium‐substituted β‐TCP was the main crystalline phase of the composites beside some calcium silicate crystallized in the bioactive glass phase. Plenty precipitation of calcium phosphate layer onto the surfaces of the β‐TCP/bioactive glass composites soaked in SBF indicated superior bioactivity of these materials compared to pure β‐TCP without any precipitation. The ability of β‐TCP/bioactive glass composites to support the growth of human osteoblastic cells was considerably better than that of pure β‐TCP. These results may be used to indicate which compositions and processing conditions can provide appropriate materials for hard tissue regeneration. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009