bluuebunny/crossref_metadata_2025_split huggingface dataset:
Electrophysiologically Dissociating Episodic Preretrieval Processing
Authors: Bridger Emma K. , Mecklinger Axel 
Published: October 2011

Abstract
Contrasts between ERPs elicited by new items from tests with distinct episodic retrieval requirements index preretrieval processing. Preretrieval operations are thought to facilitate the recovery of task-relevant information because they have been shown to correlate with response accuracy in tasks in which prioritizing the retrieval of this information could be a useful strategy. This claim was tested here by contrasting new item ERPs from two retrieval tasks, each designed to explicitly require the recovery of a different kind of mnemonic information. New item ERPs differed from 400 msec poststimulus, but the distribution of these effects varied markedly, depending upon participants' response accuracy: A protracted posteriorly located effect was present for higher performing participants, whereas an anteriorly distributed effect occurred for lower performing participants. The magnitude of the posterior effect from 400 to 800 msec correlated with response accuracy, supporting the claim that preretrieval processes facilitate the recovery of task-relevant information. Additional contrasts between ERPs from these tasks and an old/new recognition task operating as a relative baseline revealed task-specific effects with nonoverlapping scalp topographies, in line with the assumption that these new item ERP effects reflect qualitatively distinct retrieval operations. Similarities in these effects were also used to reason about preretrieval processes related to the general requirement to recover contextual details. These insights, alongside the distinct pattern of effects for the two accuracy groups, reveal the multifarious nature of preretrieval processing while indicating that only some of these classes of operation are systematically related to response accuracy in recognition memory tasks.
A Novel Approach for the Detection of Inhomogeneities Affecting Climate Time Series
Authors: Toreti Andrea , Kuglitsch Franz G. , Xoplaki Elena , Luterbacher Jürg 
Published: October 2011

AbstractSudden changes caused by nonclimatic factors (inhomogeneities) usually affect instrumental time series of climate variables. To perform robust climate analyses based on observations, a proper identification of such changes is necessary. Here, an approach (named the “GAHMDI” method, after its components and purpose) that is based on a genetic algorithm and hidden Markov models is proposed for detection of inhomogeneities caused by changes in the mean and variance. Simulated series and a case study (winter precipitation from a weather station located in Milan, Italy) are set up to compare GAHMDI with existing methodologies and to highlight its features. For the identification of a single changepoint, GAHMDI performs similarly to other methods (e.g., standard normal homogeneity test). However, for the identification of multiple inhomogeneities and changes in variance, GAHMDI returns better results than three widespread methods by avoiding overdetection. For future applications and research in the homogenization of climate datasets (temperature and precipitation) the use of GAHMDI is encouraged, preferably in combination with another detection procedure (e.g., the method of Caussinus and Mestre) when metadata are not available. Since GAHMDI is developed in the generic context of time series segmentation, it can be applied to series of generic variables—for instance, those related to economics, biology, and informatics.
Prediction of the low cycle fatigue regime of the S–N curve with application to an aluminium alloy
Authors: Jan M M , Gaenser H-P , Eichlseder W 
Published: October 2011

Engineering estimates for the prediction of fatigue life is an important consideration at the design stage of components. Conventional approach of dimensioning against cyclic loading consists of comparing the stress amplitudes against the life time. Similarly, when a component is under high loading, a strain-life methodology is adopted. In both cases, extensive experimental efforts are required for obtaining the stress-life or strain-life curves. Therefore, it is important to develop a methodology for obtaining the fatigue life of a material to obtain a basic fatigue life data for design engineers. This study focuses on the fatigue life of a material in the region of low cycle fatigue. A method is proposed for obtaining an estimate for the fatigue life of material under high loading from the results of the high cycle fatigue and static properties of the material within the stress-based diagram. The concept is applied to a wrought aluminium alloy typically used for cryogenic applications.
Using National Surveys to Measure Dynamic U.S. State Public Opinion: A Guideline for Scholars and an Application
Authors: Pacheco Julianna 
Published: October 2011

AbstractThe author improves on the measurement of U.S. state public opinion by (1) applying previous methods used only on cross-sectional data to create dynamic measures of state public opinion and (2) providing a systematic comparison of the performance of the various methodological approaches on these dynamic measures. The author shows that scholars can use multilevel regression, imputation, and poststratification (MRP) coupled with a simple moving average to measure state public opinion over time. Compared to aggregation, the MRP approach has less error and is more reliable, particularly for the less populated states. The author shows the applicability of the MRP approach by measuring and validating state partisanship and state ideology over time. The validated measures are available for public use. Armed with a method to measure state public opinion over time, scholars can begin to more fully understand the dynamic relationship between public opinion and policy in the U.S. states.
Regenerating islet-derived 3-alpha is a biomarker of gastrointestinal graft-versus-host disease
Authors: Ferrara James L. M. , Harris Andrew C. , Greenson Joel K. , Braun Thomas M. , Holler Ernst , Teshima Takanori , Levine John E. , Choi Sung W. J. , Huber Elisabeth , Landfried Karin , Akashi Koichi , Vander Lugt Mark , Reddy Pavan , Chin Alice , Zhang Qing , Hanash Samir , Paczesny Sophie 
Published: October 2011

AbstractThere are no plasma biomarkers specific for GVHD of the gastrointestinal (GI) tract, the GVHD target organ most associated with nonrelapse mortality (NRM) following hematopoietic cell transplantation (HCT). Using an unbiased, large-scale, quantitative proteomic discovery approach to identify candidate biomarkers that were increased in plasma from HCT patients with GI GVHD, 74 proteins were increased at least 2-fold; 5 were of GI origin. We validated the lead candidate, REG3α, by ELISA in samples from 1014 HCT patients from 3 transplantation centers. Plasma REG3α concentrations were 3-fold higher in patients at GI GVHD onset than in all other patients and correlated most closely with lower GI GVHD. REG3α concentrations at GVHD onset predicted response to therapy at 4 weeks, 1-year NRM, and 1-year survival (P ≤ .001). In a multivariate analysis, advanced clinical stage, severe histologic damage, and high REG3α concentrations at GVHD diagnosis independently predicted 1-year NRM, which progressively increased with higher numbers of onset risk factors present: 25% for patients with 0 risk factors to 86% with 3 risk factors present (P < .001). REG3α is a plasma biomarker of GI GVHD that can be combined with clinical stage and histologic grade to improve risk stratification of patients.
Notch signaling in acute lymphoblastic leukemia: any role for stromal microenvironment?
Authors: Kamdje Armel Hervé Nwabo , Krampera Mauro 
Published: October 2011

AbstractNotch signaling pathway regulates many different events of embryonic and adult development; among them, Notch plays an essential role in the onset of hematopoietic stem cells and influences multiple maturation steps of developing lymphoid and myeloid cells. Deregulation of Notch signaling determines several human disorders, including cancer. In the last decade it became evident that Notch signaling plays pivotal roles in the onset and development of T- and B-cell acute lymphoblastic leukemia by regulating the intracellular molecular pathways involved in leukemia cell survival and proliferation. On the other hand, bone marrow stromal cells are equally necessary for leukemia cell survival by preventing blast cell apoptosis and favoring their reciprocal interactions and cross-talk with bone marrow microenvironment. Quite surprisingly, the link between Notch signaling pathway and bone marrow stromal cells in acute lymphoblastic leukemia has been pointed out only recently. In fact, bone marrow stromal cells express Notch receptors and ligands, through which they can interact with and influence normal and leukemia T- and B-cell survival. Here, the data concerning the development of T- and B-cell acute lymphoblastic leukemia has been critically reviewed in light of the most recent findings on Notch signaling in stromal microenvironment.
The MDCK variety pack: choosing the right strain
Authors: Dukes Joseph D , Whitley Paul , Chalmers Andrew D 
Published: October 2011

Abstract
The MDCK cell line provides a tractable model for studying protein trafficking, polarity and junctions (tight, adherens, desmosome and gap) in epithelial cells. However, there are many different strains of MDCK cells available, including the parental line, MDCK I, MDCK II, MDCK.1, MDCK.2, superdome and supertube, making it difficult for new researchers to decide which strain to use. Furthermore, there is often inadequate reporting of strain types and where cells were obtained from in the literature. This review aims to provide new researchers with a guide to the different MDCK strains and a directory of where they can be obtained. We also hope to encourage experienced researchers to report the stain and origin of their MDCK cells.
Association of chemerin mRNA expression in human epicardial adipose tissue with coronary atherosclerosis
Authors: Gao Xiuying , Mi Shuhua , Zhang Fuzhuang , Gong Fengying , Lai Yongqiang , Gao Feng , Zhang Xiaoxia , Wang Linjie , Tao Hong 
Published: October 2011

Abstract

Background
Growing evidence suggests that epicardial adipose tissue (EAT) may play a key role in the pathogenesis and development of coronary artery disease (CAD) by producing several inflammatory adipokines. Chemerin, a novel adipokine, has been reported to be involved in regulating immune responses and glucolipid metabolism. Given these properties, chemerin may provide an interesting link between obesity, inflammation and atherosclerosis. In this study, we sought to determine the relationship of chemerin expression in EAT and the severity of coronary atherosclerosis in Han Chinese patients.

Methods
Serums and adipose tissue biopsies (epicardial and thoracic subcutaneous) were obtained from CAD (n = 37) and NCAD (n = 16) patients undergoing elective cardiac surgery. Gensini score was used to assess the severity of CAD. Serum levels of chemerin, adiponectin and insulin were measured by ELISA. Chemerin protein expression in adipose tissue was detected by immunohistochemistry. The mRNA levels of chemerin, chemR23, adiponectin and TNF-alpha in adipose tissue were detected by RT-PCR.

Results
We found that EAT of CAD group showed significantly higher levels of chemerin and TNF-alpha mRNA, and significantly lower level of adiponectin mRNA than that of NCAD patients. In CAD group, significantly higher levels of chemerin mRNA and protein were observed in EAT than in paired subcutaneous adipose tissue (SAT), whereas such significant difference was not found in NCAD group. Chemerin mRNA expression in EAT was positively correlated with Gensini score (r = 0.365, P < 0.05), moreover, this correlation remained statistically significant (r = 0.357, P < 0.05) after adjusting for age, gender, BMI and waist circumference. Chemerin mRNA expression in EAT was also positively correlated with BMI (r = 0.305, P < 0.05), waist circumference (r = 0.384, P < 0.01), fasting blood glucose (r = 0.334, P < 0.05) and negatively correlated with adiponectin mRNA expression in EAT (r = -0.322, P < 0.05). However, there were no significant differences in the serum levels of chemerin or adiponectin between the two groups. Likewise, neither serum chemerin nor serum adiponectin was associated with Gensini score (P > 0.05).

Conclusions
The expressions of chemerin mRNA and protein are significantly higher in EAT from patients with CAD in Han Chinese patients. Furthermore, the severity of coronary atherosclerosis is positive correlated with the level of chemerin mRNA in EAT rather than its circulating level.
Heterologous expression, biochemical characterization, and overproduction of alkaline α-amylase from Bacillus alcalophilus in Bacillus subtilis
Authors: Yang Haiquan , Liu Long , Li Jianghua , Du Guocheng , Chen Jian 
Published: October 2011

Abstract

Background
Alkaline α-amylases have potential applications for hydrolyzing starch under high pH conditions in the starch and textile industries and as ingredients in detergents for automatic dishwashers and laundries. While the alkaline α-amylase gains increased industrial interest, the yield of alkaline α-amylases from wild-type microbes is low, and the combination of genetic engineering and process optimization is necessary to achieve the overproduction of alkaline α-amylase.

Results
The alkaline α-amylase gene from Bacillus alcalophilus JN21 (CCTCC NO. M 2011229) was cloned and expressed in Bacillus subtilis strain WB600 with vector pMA5. The recombinant alkaline α-amylase was stable at pH from 7.0 to 11.0 and temperature below 40°C. The optimum pH and temperature of alkaline α-amylase was 9.0 and 50°C, respectively. Using soluble starch as the substrate, the K
m and V
max of alkaline α-amylase were 9.64 g/L and 0.80 g/(L·min), respectively. The effects of medium compositions (starch, peptone, and soybean meal) and temperature on the recombinant production of alkaline α-amylase in B. subtilis were investigated. Under the optimal conditions (starch concentration 0.6% (w/v), peptone concentration 1.45% (w/v), soybean meal concentration 1.3% (w/v), and temperature 37°C), the highest yield of alkaline α-amylase reached 415 U/mL. The yield of alkaline α-amylase in a 3-L fermentor reached 441 U/mL, which was 79 times that of native alkaline α-amylase from B. alcalophilus JN21.

Conclusions
This is the first report concerning the heterologous expression of alkaline α-amylase in B. subtilis, and the obtained results make it feasible to achieve the industrial production of alkaline α-amylase with the recombinant B. subtilis.
Differential expression of alpha 2 macroglobulin in response to dietylstilbestrol and in ovarian carcinomas in chickens
Authors: Lim Whasun , Jeong Wooyoung , Kim Ji-Hye , Lee Jin-Young , Kim Jinyoung , Bazer Fuller W , Han Jae Yong , Song Gwonhwa 
Published: October 2011

Abstract

Background
Alpha 2 macroglobulin (A2M; also known as ovostatin), a homotetrameric protein with four disulfide-linked subunits, has the unique feature of inactivating/inhibiting most known proteases including serine-, threonine-, cysteine-, aspartic- and metalloproteases. In chickens, A2M has been identified and characterized biochemically, but little is known of its functional role(s) in the oviduct, hormonal regulation of expression or its expression in ovarian carcinomas in chickens. Therefore, we investigated estrogen regulation of A2M gene expression during development of the chicken oviduct, and its expression in normal and cancerous ovaries from chickens.

Methods
To determine tissue-specific expression of A2M in chickens, we collected various organs from male and female chickens and performed RT-PCR analyses. To examine A2M gene expression in the oviduct of 1-week-old female chicks that received a subcutaneous implant of 15 mg DES in the abdominal region for 20 days, we performed RT-PCR, qPCR and in situ hybridization analyses using cDNAs from control- (n = 5) and DES-treated oviducts (n = 5), and then each segment of the oviduct from DES-treated chicks. To determine if A2M is a biomarker of ovarian cancer in hens, we collected cancerous (n = 10) ovaries from a total of 136 chickens which had completely stopped egg-laying and performed RT-PCR and in situ hybridization analyses.

Results
We found that A2M is most abundant in the chicken oviduct, specifically luminal (LE) and glandular epithelia (GE), but it was not detected in any other tissues of either sex. We then determined that DES (dietylstilbestrol, a synthetic nonsteroidal estrogen) increased A2M mRNA only in LE and GE of the oviduct of chicks. Further, expression of A2M was most abundant in GE of endometrioid adenocarcinoma of cancerous, but not normal ovaries of hens.

Conclusions
Collectively, results of the present study indicate that A2M is novel estrogen-stimulated gene expressed in LE and GE of the chicken oviduct and may be used for monitoring effects of therapies for ovarian cancer in laying hens.
Preparation of Pt Ag alloy nanoisland/graphene hybrid composites and its high stability and catalytic activity in methanol electro-oxidation
Authors: Feng Lili , Gao Guo , Huang Peng , Wang Xiansong , Zhang Chunlei , Zhang Jiali , Guo Shouwu , Cui Daxiang 
Published: October 2011

AbstractIn this article, PtAg alloy nanoislands/graphene hybrid composites were prepared based on the self-organization of Au@PtAg nanorods on graphene sheets. Graphite oxides (GO) were prepared and separated to individual sheets using Hummer's method. Graphene nano-sheets were prepared by chemical reduction with hydrazine. The prepared PtAg alloy nanomaterial and the hybrid composites with graphene were characterized by SEM, TEM, and zeta potential measurements. It is confirmed that the prepared Au@PtAg alloy nanorods/graphene hybrid composites own good catalytic function for methanol electro-oxidation by cyclic voltammograms measurements, and exhibited higher catalytic activity and more stability than pure Au@Pt nanorods and Au@AgPt alloy nanorods. In conclusion, the prepared PtAg alloy nanoislands/graphene hybrid composites own high stability and catalytic activity in methanol electro-oxidation, so that it is one kind of high-performance catalyst, and has great potential in applications such as methanol fuel cells in near future.
TSH Compensates Thyroid-Specific IGF-I Receptor Knockout and Causes Papillary Thyroid Hyperplasia
Authors: Müller Kathrin , Führer Dagmar , Mittag Jens , Klöting Nora , Blüher Matthias , Weiss Roy E. , Many Marie-Christine , Schmid Kurt Werner , Krohn Knut , Führer-Sakel Dagmar 
Published: October 2011

Abstract
Although TSH stimulates all aspects of thyroid physiology IGF-I signaling through a tyrosine kinase-containing transmembrane receptor exhibits a permissive impact on TSH action. To better understand the importance of the IGF-I receptor in the thyroid in vivo, we inactivated the Igf1r with a Tg promoter-driven Cre-lox system in mice. We studied male and female mice with thyroidal wild-type, Igf1r+/−, and Igf1r−/− genotypes. Targeted Igf1r inactivation did transiently reduce thyroid hormone levels and significantly increased TSH levels in both heterozygous and homozygous mice without affecting thyroid weight. Histological analysis of thyroid tissue with Igf1r inactivation revealed hyperplasia and heterogeneous follicle structure. From 4 months of age, we detected papillary thyroid architecture in heterozygous and homozygous mice. We also noted increased body weight of male mice with a homozygous thyroidal null mutation in the Igf1r locus, compared with wild-type mice, respectively. A decrease of mRNA and protein for thyroid peroxidase and increased mRNA and protein for IGF-II receptor but no significant mRNA changes for the insulin receptor, the TSH receptor, and the sodium-iodide-symporter in both Igf1r+/− and Igf1r−/− mice were detected. Our results suggest that the strong increase of TSH benefits papillary thyroid hyperplasia and completely compensates the loss of IGF-I receptor signaling at the level of thyroid hormones without significant increase in thyroid weight. This could indicate that the IGF-I receptor signaling is less essential for thyroid hormone synthesis but maintains homeostasis and normal thyroid morphogenesis.
An Alanic Marginal Note and The Exact Date of John II's Battle with the Pechenegs
Authors: Ivanov Sergey A. , Lubotsky Alexandr 
Published: October 2011

Abstract
The Greek Prophetologion manuscript Q12 from the library of the Academy of Sciences in St. Petersburg, copied in 1275, contains some thirty marginal notes written in Alanic, a pre-stage of Ossetic.
On leaf 100r, the glossator provided the Greek heading τ παραμο(ν) τς μέ(σο) ν´ (i.e. μεσοπεντηκοστς), ‘Eve of Mid-Pentecost’, with a gloss πητζινάκ χουτζάου πάν which most probably means ‘Pecheneg Sunday’. A Pecheneg festival established after the decisive victory of John II over the Pechenegs is attested by both Nicetas Choniates and Ecloga Basilicorum. It is no wonder that this festival reached distant Alania, since the 12th century was a heyday for the Orthodox Christianity in the Northern Caucasus.
The battle took place near Beroea in spring of the “fifth year” of John II's reign. The majority of historians tend to opt for 1122, but we would not exclude 1123 either. Whatever the year, the marginal note from Q12 may help in establishing the day of the battle, which is not mentioned either in Greek or in Scandinavian sources. If the battle of Beroea took place in 1122, it happened either on the 20th–22nd or on the 24th–26th of April. If the battle was fought in 1123, the day of the victory could then be either the 10th–12th or the 14th–16th of May.
Cultures of Participation as a Persuasive Technology
Authors: Dick Holger , Zietz Jason 
Published: October 2011

Abstract
Traditionell werden mit Persuasiven Technologien dedizierte Systeme beschrieben, die mit der Absicht entwickelt wurden, Nutzer zu bestimmten Aktivitäten zu bewegen. Hier präsentieren wir ein Cultures of Participation Framework, das erklärt, wie aus Socio-Technischen Systemen (STS) — oft unabsichtlich — Persuasive Technologien werden. Wir geben Richtlinien, wie dieses Framework genutzt werden kann, um STS zu entwickeln, die Nutzer in einer positiven Weise beeinflussen. Wir illustrieren das Framework anhand von EMPIRE, einem System das Menschen dazu motiviert und darin unterstützt, Energieverschwendungen zu reduzieren.
Der Digitale Untersetzer — Änderung des Trinkverhaltens durch Reflexion
Authors: Buchholz Hagen , Prinz Wolfgang 
Published: October 2011

Zusammenfassung
Das im vorliegenden Text beschriebene persuasive System soll Menschen davon überzeugen, ihr Trinkverhalten zu ändern. Anhand eines ersten Prototyps wurde getestet, ob eine einfache Rückmeldung über die täglich aufgenommene Menge an Flüssigkeit ausreicht. Erste Tests gaben Hinweise darauf, dass die Installation des Systems bei jedem Nutzer zu einer Auseinandersetzung mit dem Thema führte. Zumindest kurzfristig war bei allen Probanden eine Änderung zu erkennen. Nach einer gewissen Zeit schien sich ein Großteil der Testpersonen jedoch an das System zu gewöhnen. Die direkte Wirkung des Systems ging zurück. Eine einfache Darstellung der Tagesaufnahme an Flüssigkeit, scheint in diesem Zusammenhang, langfristig keinen direkten Einfluss nehmen zu können.