bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recent Advances in Co-Condensation and Co-Aggregation of Amyloid Proteins Linked to Neurodegenerative Diseases
Authors: Zhang Xuefeng , Chen Yujie , Tan Yuan , Pan Tong , Wei Guanghong 
Published: February 2025

Abstract:
The misfolding and aggregation of amyloid proteins are closely associated with a range
of neurodegenerative diseases. Liquid-liquid phase separation (LLPS) can initiate the aggregation
of proteins, indicating that LLPS may serve as an alternative pathway for the pathological aggregation
of amyloid proteins. The co-occurrence of two or more amyloid pathologies has been observed
in extensive pathophysiological studies and is linked to faster disease progression. The co-
LLPS (also known as co-condensation) and co-aggregation of different disease-related proteins
have been proposed as a potential molecular mechanism for combined neuropathology. Here, we
reviewed the current state of knowledge regarding the co-aggregation and co-condensation of various
amyloid proteins, including Aβ, tau, α-synuclein, TDP-43, FUS, and hnRNPA/B protein family,
C9orf72 dipeptide repeats and prion protein. We briefly introduced the epidemiological correlation
among different neurodegenerative diseases and specifically presented recent experimental
findings about co-aggregation and co-condensation of two different amyloid proteins. Additionally,
we discussed computational studies focusing on the molecular interactions between amyloid
proteins to offer mechanistic insights into the co-LLPS and co-aggregation processes. This review
provides an overview of the synergistic interactions between different disease-related proteins,
which is helpful for understanding the mechanisms of combined neuropathology and developing
targeted therapeutic strategies.
Age- and Genotype-Dependent Effects of Chronic Nicotine on Presenilin1/2 Double Knockout Mice: From Behavior to Molecular Pathways
Authors: Si Youwen , Meng Bo , Qi Feiyan 
Published: February 2025

Introduction:
The potential therapeutic role of nicotine in Alzheimer's disease (AD)
remains controversial, particularly regarding its age-dependent effects and underlying mechanisms.

Method:
This study investigated the impact of chronic nicotine administration on cognitive function
and molecular pathways in Presenilin 1/2 double knockout (DKO) mice, an amyloid-β-independent
model of AD. Three-month-old and eight-month-old DKO and wild-type (WT) mice received
oral nicotine treatment (100 μg/ml) for three months. Behavioral assessments revealed that
while the 6-month-old cohort showed no significant differences between nicotine-treated and control
groups regardless of genotype, nicotine improved contextual fear memory in 11-month-old
DKO mice but impaired nest-building ability and cued fear memory in age-matched WT controls.
Transcriptome analysis of the prefrontal cortex identified distinct molecular responses to nicotine
between genotypes.

Result:
In DKO mice, nicotine modulated neuropeptide signaling and reduced astrocyte activation,
while in WT mice, it disrupted cytokine-cytokine receptor interaction and neuroactive ligand-
receptor interaction pathways. Western blot analysis revealed that nicotine treatment significantly
reduced tau hyperphosphorylation and GFAP expression in 11-month-old DKO mice,
which was further confirmed by immunohistochemistry showing decreased astrocyte activation in
multiple brain regions.

Conclusion:
These findings demonstrate that nicotine's effects on cognition and molecular pathways
are both age- and genotype-dependent, suggesting its therapeutic potential may be limited to
specific stages of neurodegeneration while potentially having adverse effects in healthy aging
brains.
Determining the Interaction in a Drug Combination using the Dose-based or Effect-based Method
Authors: Yu Tinghe , Yu Tianyan , Li Xinya , Li Min 
Published: February 2025

Abstract:
The interaction in a drug combination can be assessed using either the method of Chou or
Jin’s method. The combination index in the former (i.e., CI-C) is calculated based on doses, while
the latter (i.e., CI-J) is based on effects. This perspective demonstrates a correlation between 1/CI-C
and CI-J when applied to both released and simulated data. Thus, 1/CI-C and CI-J are functionally
equivalent for evaluating drug interaction. Combining these two indices is preferred; consistency
shows a reliable verdict, and inconsistency indicates a requirement for further analyses. However, it
has been observed that evaluating released data raises certain concerns.
Computational Evaluation of Bioactive Compounds from Bryophyllum pinnatum as a Novel GSK3B Inhibitor in Alzheimer's Disease
Authors: Babalola Olorunfemi Oyewole , Ottu Paul Olamide , Akinola Olufemi Adebisi , Kehinde Idayat Oyinkansola , Oluwatobiloba Ayodeji Folasade , Oluwamodupe Cecilia , Iwaloye Opeyemi , Elekofehinti Olusola Olalekan 
Published: February 2025

Background:
Glycogen synthase kinase 3-beta (GSK3-β) is a protein that is linked to the
formation of amyloid-beta (Aβ) plaques and neurofibrillary tangles, both of which are characteristic
of Alzheimer's disease. The enzyme's hyperactivity phosphorylates tau proteins, forming neurofibrillary
tangles that impair material transport between axons and dendrites and disrupt neuronal
function. GSK3-β impacts amyloid precursor protein production, causing Aβ accumulation and neurodegeneration.

Aim/Objective:
This study aimed to investigate the inhibitory mechanism of natural compounds
from Bryophyllum pinnatum against GSK3-β.

Methods:
Computational approach, extra precision glide docking, and the Maestro molecular interface
of Schrodinger suites were utilized to determine the binding free energy of the compounds
against the prepared GSK3-β. The compounds' ADME parameters and Lipinski's rule of five were
also evaluated. Using AutoQSAR, predictive models were constructed for both protein targets.

Results:
Three hit compounds (kaempferol, quercetin, and 5,7,4'-trihydroxy-3,8-dimethoxyflavone)
were found in this study. These compounds met the recommended range for the specified ADME
parameters and passed the rule of five. Additionally, the hit compounds' predicted pIC50 values
were promising.

Conclusion:
Our study suggests that the investigated compounds can be used to design GSK3-β inhibitors.
Unlocking the Biological Potential of 2-Pyridones: Synthesis, Antioxidant and Antimicrobial Activity of N-Phenacylated 5/6-Chloro-2-pyridones
Authors: Sangwan Sarita , Chauhan Sonu , Yadav Neelam , Kumar Ravi , Duhan Anil , Malik Vinod 
Published: February 2025

Aim:
A simple and efficient synthesis of 14 new (9a-9n) N-phenacyl-2-pyridones with
good yields (up to 75%), is reported. The synthesized derivatives were screened for their in vitro
radical scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH), their in vitro antimicrobial
potential was tested against human pathogenic bacterial strains, including Bacillus cereus,
Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa, as well as the fungal strain
Candida albicans.

Method:
All compounds displayed modest antioxidant activity, with compound 9b being the most
potent in the DPPH radical scavenging assay. Most of the synthesized compounds exhibited good
to excellent antimicrobial activity, however, the compounds (9d, and 9b) showed maximum inhibition
zone diameters of 18.75, and 18.25mm respectively, demonstrating better antimicrobial potential
than the standard drug streptomycin against Staphylococcus aureus.

Result:
However, the compound 9f was found most effective against Pseudomonas aeruginosa
with a 23.25 mm zone of inhibition against a 17.50 mm zone of inhibition of the standard, streptomycin.
Molecular docking of the compounds 9d and 9f with tyrosyl-tRNA synthetase revealed
good binding with the target.

Conclusion:
The electron-withdrawing substituents on the aryl ring of synthesized N-phenacyl-2-
pyridones improved the antioxidant activity, however, for Gram-positive bacteria, less lipophilic or
more hydrophilic substituents, such as halogens, displayed better antimicrobial activity. Similarly,
it was the more lipophilic substitutions on the aryl ring that improved the antimicrobial activity
against Gram-negative bacteria.
The Prognostic Role of Interferon Gamma-inducible Protein 30 in Clear Cell Renal Cell Carcinoma with Immune Infiltrates
Authors: Xu Wu , Wu Taihong , Liu Yufeng , Luo Yang , Liu Dawei , Yan Lingfei , Li Qing , Wang Tao 
Published: February 2025

Background:
Recent research has demonstrated the significance of Interferon Gamma-
Inducible Protein 30 (IFI30), an interferon gamma-induced protein, in the immune response to
cancerous growths. However, the relationship between IFI30 expression levels, patient prognosis,
and tumor-infiltrating lymphocytes in clear cell renal cell carcinoma (ccRCC) remains inadequately
defined.

Methods:
To ascertain the potential link between IFI30 expression, clinical data, and overall survival
(OS) in ccRCC patients, we employed diverse databases, which include TCGA, Gene Expression
Profiling Interaction Analysis (GEPIA), and UALCAN. Furthermore, an in-depth analysis of
the link between tumor-infiltrating immune cells (TIIC) and IFI30 was carried out using the
TIMER, GEPIA, and TISIDB databases. Immunohistochemistry (IHC) was utilized to identify the
IFI30 and PD-1 expression levels in a tissue microarray. Patents about molecular classification
and drugs in ccRCC were reviewed through Worldwide Espacenet®.

Results:
The expression of IFI30 demonstrated a strong association with sample type, lymph node
stage, tumor grade, and cancer stage. Elevated IFI30 expression was linked to unfavorable Disease-
Specific Survival (DSS) and Overall Survival (OS) outcomes (p <0.01). Furthermore, overexpression
of IFI30 was strongly linked to immunomodulatory molecules, chemokines, and increased
infiltration of regulatory T cells (Tregs), natural killer (NK) CD56 cells, T helper 1 (Th1)
cells, cytotoxic T cells, and T helper cells. IHC analysis confirmed a robust correlation between
IFI30 and PD-1 expression.

Conclusion:
IFI30 is a prognostic biomarker for ccRCC patients. Targeting IFI30 may provide
new strategies for cancer therapy and improve the prognosis of ccRCC patients.
Development and Prospects of Transparent Working Face Construction Technology for Intelligent Coal Mining
Authors: Li Wei , Liu Zaibin , Fan Tao , Wenming Liu , Wang Xiaohui , Yan Junsheng , Wang Huahui 
Published: February 2025

Background:
As global energy demand continues to rise, coal remains a critical energy
resource. However, traditional coal mining methods face significant challenges such as low safety,
environmental degradation, and resource wastage. In addition, to address these issues, intelligent
coal mining technologies have emerged, with the construction of transparent working faces receiving
significant attention as a key component.

Objective:
This paper aims to explore and summarize the key technologies and methods involved
in the construction of transparent working faces in coal mining, with a focus on enhancing mining
safety, efficiency, and resource utilization.

Methods:
The research comprehensively reviews the principles and applications of various geological
exploration technologies, including surface 3D seismic techniques, channel wave seismic exploration,
mine resistivity methods, directional drilling, borehole transient electromagnetics, and
borehole seismic CT. Additionally, it discusses critical technologies such as intelligent sensing,
multi-source heterogeneous data fusion, and multi-attribute dynamic visualization modeling.

Results:
The study finds that surface 3D seismic techniques are effective for detecting geological
structures within the working face, while mine geophysical prospecting methods like channel
wave seismic and resistivity methods offer high precision and efficiency. Directional drilling and
borehole techniques provide high detection accuracy for concealed structures and anomalies. Furthermore,
the integration of multi-source data significantly enhances the accuracy and real-time capabilities
of geological modeling.

Conclusion:
The construction of transparent working faces plays a crucial role in the sustainable
development of the coal industry by improving mining safety, efficiency, and resource utilization.
Future advancements will focus on algorithm optimization, the application of machine learning,
and the development of refined multi-source data integration platforms.
A Case of Idiopathic Colonic Varices: A Rare Cause of Hematochezia
Authors: Patel Dhruvkumar , Patel Maitri M. , Prajapati Poojan , Patel Lalitkumar , Hussain Kabir , Zia Hassaan Ashraf , Patel Mukundkumar V. 
Published: February 2025

Background:
Colonic varices, typically associated with portal hypertension, are a rare cause of lower gastrointestinal bleeding, with idiopathic colonic varices being exceed-ingly uncommon.

Case Presentation:
We report a case of a 36-year-old male who presented with acute ab-dominal pain, hematochezia (blood-streaked stools), and severe iron deficiency anemia. The patient’s past medical history was unremarkable, with no known liver disease, alcohol use, or family history of gastrointestinal disorders. Esophagogastroduodenoscopy (EGD) and colon-oscopy revealed dilated varices in the colon, though no active bleeding was observed. Com-prehensive laboratory investigations, including liver function tests, coagulation profiles, and viral hepatitis serologies, were within normal limits. Imaging studies, including contrast-en-hanced computed tomography (CT) of the abdomen and Doppler ultrasonography, ruled out portal hypertension and other systemic pathologies. With no identifiable underlying cause, a diagnosis of idiopathic colonic varices was made.

Treatment and Outcome:
The patient was managed conservatively with packed red blood cell transfusions and oral iron supplementation to correct the anemia. A follow-up colonoscopy at three months showed no progression of varices, and the patient remained asymptomatic.

Conclusion:
This case highlights the importance of considering idiopathic colonic varices in patients with hematochezia and iron deficiency anemia, even in the absence of portal hyper-tension. A thorough diagnostic workup, including endoscopy and imaging, is essential to ex-clude more common causes and arrive at this rare diagnosis.
IMPLEMENTASI KONSEP LINK AND MATCH 8+i PADA PROGRAM SMK PUSAT KEUNGGULAN DI SMK NEGERI 1 METRO LAMPUNG JURUSAN PEMASARAN UNTUK MENINGKATKAN MUTU LULUSAN
Authors: Astuti Vivin Refi , Dami Sudarman , Dacholfany M Ihsan 
Published: February 2025

SMK Negeri 1 Metro Lampung adalah salah satu SMK yang ada di kecamatan Metro Timur Kota Metro Provinsi Lampung. Di SMK ini terdapat 6 Jurusan yaitu Jurusan Pemasaran, Akuntansi dan Keuangan Lembaga, Otomatisasi Tata Kelola Perkantoran, Tata Boga, Perhotelan, Usaha Perjalanan Wisata. Sebagai wujud pelaksanaan program yaitu kemitraan link and match secara menyeluruh sesuai kesepakatan dengan Dunia Usaha, Dunia Industri, dan Dunia Kerja (DUDIKA), dalam hal ini SMK Negeri 1 Metro Lampung menjalin kerjasama dengan PT. Sumber Alfaria Trijaya sebagai pihak Dunia Usaha, Dunia Industri, dan Dunia Kerja (DUDIKA). Penelitian bertujuan untuk mengetahui pelaksanaan konsep link and match 8+I di SMK Negeri 1 Metro Lampung Jurusan Pemasaran dalam meningkatkan mutu lulusan. Penelitian ini menggunakan pendekatan kualitatif dengan teknik pengumpulan data yang digunakan yaitu observasi, wawancara, dan dokumentasi. Selanjutnya data yang diperoleh setelah dikumpulkan tersebut dianalisis melalui teknik reduksi data, display data, dan penarikan Kesimpulan. Berdasarkan analisis yang diperoleh, Sebagai sekolah yang ditetapkan sebagai SMK Pusat Keunggulan pada jurusan Pemasaran, SMK Negeri 1 Metro Lampung telah melaksanakan konsep link and match 8+I dengan baik, walaupun ada beberapa kegiatan yang kurang maksimal pelaksanaannya. Implementasi konsep link and match 8+i pada SMK Negeri 1 Metro Lampung berhasil dicapai melalui proses belajar mengajar, serta kerjasama dengan pihak Industri, dimana semua program kegiatan sekolah dalam penerapan konsep Link and match 8+i mengikutsertakan Industri. Kerjasama antara sekolah dan Industri mencakup sinkronisasi kurikulum, peran serta guru/instruktur dari Industri, Praktik Kerja Lapangan (PKL), Pembelajaran teaching factory (tefa), dan komitmen serapan.
Measuring Financial Well Being of Tourism Labor in Bali: A Comparative Study of Generation Z and Millennial
Authors: P Wayan Agung Panca , Suasih Ni Nyoman Reni , Yasa I Gusti Wayan Murjana , Dewi Made Heny Urmila 
Published: February 2025

Tourism in Bali is a hallmark of economic prosperity, attracting millions of tourists every year due to its natural beauty, rich cultural heritage, and preserved cultural heritage. Behind this vibrant industry are millions of labors whose livelihoods depend directly or indirectly on tourism. The research explores the financial well-being of the Millennial Generation (Generation Y) and Generation Z labors in the Bali tourism sector. This method uses a quantitative approach with crosstab analysis facilitated by SPSS Statistics 25, which was collected via questionnaires from 151 tourism labors in Bali. The main findings show that although levels of financial well-being vary between generations, most respondents said they were happy with their finances. In addition, income disparities are visible, with the higher the age of tourism labors in Bali, the higher their income. However, regarding perception, financial well-being is relative, whereas generational age does not always influence financial well-being. This research contributes to a deeper understanding of the dynamics of financial well-being among Bali tourism labors. The results of this research are expected to create strategies to increase income stability, job security, and financial literacy, which are recommended to support the tourism sector sustainably and improve the overall welfare of labors in Bali.
When benign pretends to be malignant: A case report of giant cell tumor of the breast with review of literature
Authors: Aggarwal Depanshu , Nair Rishi P , Kumar Parmod , Devnani Bharti 
Published: February 2025

A giant cell tumor (GCT) of the breast is an uncommon intermediate-type soft tissue tumor with histological features resembling its osseous counterparts. To date, only 12 such cases have been documented. Our case involves a 58-year-old woman who initially presented with a breast lump progressively enlarging over six months. It was evaluated as a potential case of breast cancer. Mammography, biopsy, and, subsequently, positron emission tomography-computed tomography (PET-CT) were conducted for staging. The core biopsy specimen did not conclusively differentiate between the two suspected pathologies, i.e., GCT and Invasive breast cancer with osteoclast-like giant cells, although it was favoring the former. Following a multidisciplinary tumor board (MDTB) meeting, the patient underwent a total mastectomy and axillary lymph node dissection (TM+ALND). The large biopsy was suggestive of GCT of the breast.
We have conducted an exhaustive review of the literature on GCT of the breast, encompassing its presentation, radiological findings, differential diagnoses, and treatment modalities to formulate a structured approach for management. Additionally, we performed a statistical analysis of baseline characteristics, outcomes, and treatment modalities to succinctly summarize the current data on this subject.
Повышение помехоустойчивости в системе речевого управления
Authors: Пальков А.С. , Козлов В.В. , Бодин А.Ю. , Крамм М.Н. , Бодин О.Н. 
Published: February 2025

Обеспечение коммуникации человека с информационной радиотехнической системой является одним из самых приоритетных направлений в области информационных технологий, поэтому исследование помехоустойчивости речевого сигнала с учётом особенностей эксплуатации систем голосового управления в условиях сложной сигнально-помеховой обстановки является актуальной задачей. В статье рассматривается предварительная обработка речевых команд для систем голосового управления на основе методов бионического управления, сегментации и цифровой фильтрации. В ходе исследования была проведена экспериментальная обработка речевого сигнала, содержащего голосовую команду, путём его сегментирования выделения пассивной фазы сигнала с дальнейшей коррекцией в среде Matlab. По результатам экспериментального исследования цифрового фильтра нижних частот выявлено, что качество полученного аудиосигнала улучшается на 9 дБ по сравнению с исходным аудиосигналом.
Relevance. Facilitating human interaction with information systems ranks among the foremost priorities in the field of information technology. The investigation of speech signal parameters, considering the operational intricacies of voice control systems, along with devising a novel methodology grounded in the bionic approach and crafting dual-purpose voice control systems with enhanced attributes, are pressing objectives. The aim of this study is to improve the characteristics of dual-purpose voice control systems, employing the bionic approach, for deployment in both standard scenarios and emergency situations. Materials and Methods. The bionic method of object control involves harnessing various expressions of body vital activity as control signals for instruments, mechanisms, and devices. Our approach entails a unique sequence of procedures, incorporating additional digital processing of the speech signal. This enables the detection of interference and noise peculiar to the signal-interference environment during pauses in the speech signal, where the voice control system operates. Experimental processing of a speech signal containing a voice command was conducted in the Matlab environment, involving segmentation and isolation of the passive phase of the signal. Results and Discussion.Computational analysis revealed that the signal-to-noise ratio for the original audio signal, containing the speech command, measured at 19.2896 dB, whereas for the segmented audio signal – devoid of pauses between words – it was 25.2658 dB. Conclusion. The study validated the authors' hypothesis regarding the augmentation of noise suppression in voice control systems through the monitoring of interference during the passive phase of the speech signal. Future research endeavors are warranted to refine the segmentation and digital filtering techniques applied to speech commands in voice control systems.