bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigating the influence of perinatal fluoxetine exposure on murine gut microbial communities during pregnancy and lactation
Authors: Desorcy-Scherer Katelyn , Zuniga-Chaves Ibrahim , Reisner Maggie A. , Suen Garret , Hernandez Laura L. 
Published: June 2024

AbstractSelective Serotonin Reuptake Inhibitor (SSRI) therapy is common among perinatal populations for the treatment of mood disorders. Medications can affect diversity and composition of the gut microbiome, which plays a key role in modulating health. While previous studies have examined the effects of antidepressant exposure on the maternal gut microbiome, whether SSRI exposure affects the offspring gut microbiome is unknown. We investigated the effects of maternal fluoxetine exposure on the gut microbiome of maternal and offspring mice during pregnancy and lactation (embryonic day 10–lactation day 21; E10–L21). Stool samples collected on E17, L11, L15, and L21 were examined using 16S rRNA sequencing. Our results suggest that maternal fluoxetine exposure may result in decreased alpha diversity of the offspring gut microbiome in early life. Furthermore, we observed several genera-specific differences in the gut microbiome based on treatment, specifically of Turicibacter, Parasutterella, and Romboutsia. These findings support our understanding of gut health, as dysbiotic development of the gut microbiome has been associated with local and systemic health problems including gastrointestinal morbidities and interrupted growth patterns in infants. Future research should pursue study in human populations and those at high risk for gut microbial dysbiosis and intestinal injury.
Low-intensity pulsed ultrasound improves symptoms in patients with Buerger disease: a double-blinded, randomized, and placebo-controlled study
Authors: Mohamad Yusoff Farina , Kajikawa Masato , Yamaji Takayuki , Kishimoto Shinji , Maruhashi Tatsuya , Nakashima Ayumu , Tsuji Toshio , Higashi Yukihito 
Published: June 2024

AbstractHere we report the effects of low-intensity pulsed ultrasound (LIPUS) on symptoms in peripheral arterial disease patients with Buerger disease. A double-blinded and randomized study with active and inactive LIPUS was conducted. We assessed symptoms in leg circulation during a 24-week period of LIPUS irradiation in 12 patients with Buerger disease. Twelve patients without LIPUS irradiation served as controls. The pain intensity on visual analog score was significantly decreased after 24-week LIPUS treatment. Skin perfusion pressure was significantly increased in patients who received LIPUS treatment. There was no significant difference in symptoms and perfusion parameters in the control group. No severe adverse effects were observed in any of the patients who underwent LIPUS treatment. LIPUS is noninvasive, safe and effective option for improving symptoms in patients with Buerger disease.
Tailoring silk fibroin hydrophilicity and physicochemical properties using sugar alcohols for medical device coatings
Authors: Kaewpirom Supranee , Piboonnithikasem Sarayoot , Sroisroemsap Pongsathorn , Uttoom Sittichai , Boonsang Siridech 
Published: June 2024

AbstractThis study explores the modification of silk fibroin films for hydrophilic coating applications using various sugar alcohols. Films, prepared via solvent casting, incorporated glycerol, sorbitol, and maltitol, revealing distinctive transparency and UV absorption characteristics based on sugar alcohol chemical structures. X-ray diffraction confirmed a silk I to silk II transition influenced by sugar alcohols. Glycerol proved most effective in enhancing the β-sheet structure. The study also elucidated a conformational shift towards a β-sheet structure induced by sugar alcohols. Silk fibroin–sugar alcohol blind docking and sugar alcohol-sugar alcohol blind docking investigations were conducted utilizing the HDOCK Server. The computer simulation unveiled the significance of size and hydrogen bonding characteristics inherent in sugar alcohols, emphasizing their pivotal role in influencing interactions within silk fibroin matrices. Hydrophilicity of ozonized silicone surfaces improved through successful coating with silk fibroin films, particularly glycerol-containing ones, resulting in reduced contact angles. Strong adhesion between silk fibroin films and ozonized silicone surfaces was evident, indicating robust hydrogen bonding interactions. This comprehensive research provides crucial insights into sugar alcohols’ potential to modify silk fibroin film crystalline structures, offering valuable guidance for optimizing their design and functionality, especially in silicone coating applications.
Antitumor effect of Iso-mukaadial acetate on MCF-7 breast cancer mice xenograft model
Authors: Raphela-Choma P. P. , Lukhwareni R. , Simelane M. B. C. , Motadi L. R. , Choene M. S. 
Published: June 2024

AbstractAntitumor drugs used today have shown significant efficacy and are derived from natural products such as plants. Iso-mukaadial acetate (IMA) has previously been shown to possess anticancer properties by inducing apoptosis. The purpose of this study was to investigate the therapeutic effect of IMA in the breast cancer xenograft mice model. Female athymic nude mice were used and inoculated with breast cancer cells subcutaneously. Untreated group one served as a negative control and positive control group two (cisplatin) was administered intravenously. IMA was administered orally to group three (100 mg/kg) and group four (300 mg/kg). Blood was collected (70 μL) from the tail vein on day zero, day one and day three. Tumor regression was measured every second day and body mass was recorded each day. Estimation of serum parameters for renal indices was examined using a creatinine assay. Histopathological analysis was conducted to evaluate morphological changes of liver, kidney, and spleen tissues before and after compound administration under a fluorescence light microscope. Histopathological analysis of tumors was conducted before and after compound administration. Apoptotic analysis using the TUNEL system was conducted on liver, kidney, and spleen tissues. Tumor shrinkage and reduction in body mass were observed after treatment with IMA. Serum creatinine was slightly elevated after treatment with IMA at a dosage of 100 and 300 mg/kg. Histopathological results of the liver exhibited no changes before and after IMA while the kidney and spleen tissues showed changes in the cellular structure. IMA showed no cytotoxic effect on the tumor cells, and cell proliferation was observed. Apoptotic assay stain with TUNEL showed apoptotic cells in spleen tissue and kidney but no apoptotic cells were observed in liver tissue section treated with IMA. IMA showed clinical toxic signs that resulted in the suffering and death of the mice immediately after IMA administration. Histopathology of tumor cells showed that IMA did not inhibit cell proliferation and no cellular damage was observed. Therefore, based on the results obtained, we cannot make any definitive conclusion on the complete effect of IMA in vivo. IMA is toxic, poorly soluble, and not safe to use in animal studies. The objective of the study was not achieved, and the hypothesis was rejected.
Durability performance of alkali-activated concrete with pre-treated coarse recycled aggregates for pavements
Authors: Khan MD Ikramullah , Ram V. Vinayaka , Patel Vipulkumar Ishvarbhai 
Published: June 2024

AbstractThis study examines the effect of coarse recycled aggregates (CRAs) and processed coarse recycled aggregates (PCRAs) on the behaviour of alkali-activated concrete (AAC) before and after exposure to marine seawater and acidic environments (5% HCl and 5% H2SO4 solutions). Measurements of compressive strength and the microstructure changes were conducted over periods of 56 and 90 days to assess these effects. The experimental design included varying the replacement levels of NAs with CRAs and PCRAs from (0–100%) and using ground-granulated blast furnace slag and fly ash as constant components. In addition to durability tests, sorptivity assessments were conducted to gauge the material’s porosity and water absorption capabilities. Advanced microstructure techniques, such as scanning electron microscopy (SEM) and X-ray diffraction (XRD), were employed to detail the pre and post-exposure mineralogical and microstructural transformations within the AAC blends. The AAC mixtures incorporating PCRAs emerged as durable, showcasing better strength and a denser, more compact matrix facilitated by the synergistic formation of NASH and CASH gels after exposure to aggressive agents compared to untreated CRAs. In addition, the results show that the samples exposed to marine seawater exhibited improved mechanical performance compared to those exposed to acidic environments. The novelty of this study lies in its exploration of the effects of recycling plant-based CRAs and PCRAs on AAC for marine and acid exposure.
Left ventricular trabeculation in Hominidae: divergence of the human cardiac phenotype
Authors: Curry Bryony A. , Drane Aimee L. , Atencia Rebeca , Feltrer Yedra , Calvi Thalita , Milnes Ellie L. , Moittié Sophie , Weigold Annika , Knauf-Witzens Tobias , Sawung Kusuma Arga , Howatson Glyn , Palmer Christopher , Stembridge Mike R. , Gorzynski John E. , Eves Neil D. , Dawkins Tony G. , Shave Rob E. 
Published: June 2024

AbstractAlthough the gross morphology of the heart is conserved across mammals, subtle interspecific variations exist in the cardiac phenotype, which may reflect evolutionary divergence among closely-related species. Here, we compare the left ventricle (LV) across all extant members of the Hominidae taxon, using 2D echocardiography, to gain insight into the evolution of the human heart. We present compelling evidence that the human LV has diverged away from a more trabeculated phenotype present in all other great apes, towards a ventricular wall with proportionally greater compact myocardium, which was corroborated by post-mortem chimpanzee (Pan troglodytes) hearts. Speckle-tracking echocardiographic analyses identified a negative curvilinear relationship between the degree of trabeculation and LV systolic twist, revealing lower rotational mechanics in the trabeculated non-human great ape LV. This divergent evolution of the human heart may have facilitated the augmentation of cardiac output to support the metabolic and thermoregulatory demands of the human ecological niche.
BrainTACO: an explorable multi-scale multi-modal brain transcriptomic and connectivity data resource
Authors: Ganglberger Florian , Kargl Dominic , Töpfer Markus , Hernandez-Lallement Julien , Lawless Nathan , Fernandez-Albert Francesc , Haubensak Wulf , Bühler Katja 
Published: June 2024

AbstractExploring the relationships between genes and brain circuitry can be accelerated by joint analysis of heterogeneous datasets from 3D imaging data, anatomical data, as well as brain networks at varying scales, resolutions, and modalities. Generating an integrated view, beyond the individual resources’ original purpose, requires the fusion of these data to a common space, and a visualization that bridges the gap across scales. However, despite ever expanding datasets, few platforms for integration and exploration of this heterogeneous data exist. To this end, we present the BrainTACO (Brain Transcriptomic And Connectivity Data) resource, a selection of heterogeneous, and multi-scale neurobiological data spatially mapped onto a common, hierarchical reference space, combined via a holistic data integration scheme. To access BrainTACO, we extended BrainTrawler, a web-based visual analytics framework for spatial neurobiological data, with comparative visualizations of multiple resources. This enables gene expression dissection of brain networks with, to the best of our knowledge, an unprecedented coverage and allows for the identification of potential genetic drivers of connectivity in both mice and humans that may contribute to the discovery of dysconnectivity phenotypes. Hence, BrainTACO reduces the need for time-consuming manual data aggregation often required for computational analyses in script-based toolboxes, and supports neuroscientists by directly leveraging the data instead of preparing it.
Three-dimensional analysis reveals diverse heat wave types in Europe
Authors: Lhotka Ondřej , Kyselý Jan 
Published: June 2024

AbstractHeat waves are among the most studied atmospheric hazards but commonly investigated near-surface temperature patterns provide only limited insight into their complex structure. Here we propose and evaluate a novel approach to the analysis of heat waves as three-dimensional (3D) phenomena, employing the ERA5 reanalysis in three European regions during 1979–2022. Four types of heat waves based on their vertical cross sections of temperature anomalies are introduced: near-surface, lower-tropospheric, higher-tropospheric, and omnipresent. The individual heat wave types differ in length, predominant occurrence within summer, and soil moisture preconditioning. While near-surface heat waves may persist for more than 2 weeks, those located mainly in higher troposphere are shortest (5 days at most). This demonstrates that warm advection must be accompanied by a downward propagation of positive temperature anomalies through air subsidence and diabatic heating to maintain long-lasting heat waves. We also show that soil-moisture preconditioning is crucial for near-surface heat waves only, thus pointing to different driving mechanisms for the individual 3D heat wave types.
Managing a low urban emissions world
Authors: Lichtman Rob 
Published: June 2024

AbstractWe have largely ignored serious organization issues blocking urban greenhouse gas emissions reductions. Most urban climate measures treat decision-making as a black box, where sensible solutions are simply implemented. We need to re-examine decision-making assumptions about how city governments and businesses actually collaborate and whether current plans are credible. Significant constraints affect both groups and also limit citizens’ abilities to effectively shape policies and investments. These problems can be addressed by creating a trusted, professional, independent organization to drive innovation and implementation to reduce emissions and moderate urban inequality. This “Lowering Emissions Economy Partnership (LEEP)” can be jointly owned by stakeholders who could pool investments and recover a share of the large resource savings this approach will create. Any city could do this, drawing upon a range of start-up capital options. This reduces political and financial risks, and it can accelerate emission reductions in a more just, sustainable way.
Up to 80% of threatened and commercial species across European marine protected areas face novel climates under high emission scenario
Authors: Predragovic Milica , Assis Jorge , Sumaila U. Rashid , Gonçalves Jorge M. S. , Cvitanovic Christopher , Horta e Costa Bárbara 
Published: June 2024

AbstractMarine protected areas (MPAs) are a critical tool for safeguarding marine species and habitats for the future, though the effects of projected climate change raise concerns about their long-term success. Assessing the degree to which MPAs may be exposed to future novel climatic conditions is, therefore, crucial for informing conservation and management actions aimed at ensuring a resilient and thriving ocean in the years to come. Here, we evaluate the future exposure of 398 threatened and commercially important species to novel and extremely novel climatic conditions within European MPAs. We estimated climate novelty through multivariate analyses considering biologically meaningful distribution drivers of temperature, oxygen, pH, and primary productivity from present-day to the end of the 21st-century conditions under contrasting shared socioeconomic pathways (SSP) scenarios—low emission scenario (SSP1–1.9) and high emission scenario (SSP5–8.5). Our findings suggest that, under SSP1–1.9, ~6.5% of species and 0.5% of European MPAs will be at risk due to future novel conditions. In contrast, under SSP5–8.5, 87% of MPAs and 80% of species are projected to be at risk. Notably, up to 100% of species currently located in the MPAs of enclosed and semi-enclosed seas like the Baltic Sea and the Black Sea are projected to be exposed to novel or even extremely novel conditions. Virtually all species in most of those regions will be at risk, suggesting that even new MPAs might not be able to adequately protect them. Comparatively, the Norwegian Sea, North-East Atlantic, and western parts of the Mediterranean and North Seas are expected to be less impacted even under the high emission scenario. Overall, our study advances the understanding of the potential impacts of future climate change scenarios on threatened and commercially important marine species in European MPAs and reinforces the urgent need to meet the Paris Agreement. Our results suggest that existing approaches to marine governance in Europe may be insufficient for ensuring the success of MPAs in light of future impacts and that novel anticipatory forms of governance are needed.
Fast estimation of array shape and direction of arrival using sparse Bayesian learning for manoeuvring towed line array
Authors: Pan Xiang , Wang Haoran , Li Min , Zhou Jie , Li Yuxiao , Xu Weize 
Published: June 2024

AbstractThe sparse Bayesian learning (SBL) algorithm has demonstrated its advantage in the direction of arrival (DOA) estimation. However, it requires a lot of computational cost to iteratively estimate the SBL hyperparameters from measurement data. This paper focuses on fast estimating the array shape and DOAs using SBL for a short towed line array (TLA) during manoeuvring. A parabolic model is utilised to describe the bent TLA whose bow as a hyperparameter is estimated in the SBL iterative process. Then, the basis vector pruning strategy is considered in the iteration to reduce computational cost by neglecting the impossible directions of signal presence. The converged speed of the joint estimation algorithm is further improved by approximately calculating the posterior probability density with the message passing approach. The effectiveness of the optimising joint estimation algorithm is verified using the experimental results from South China Sea.
Fuʼcupping Physical Permeation-Enhancing Technique Enhances the Therapeutic Efficacy of Corydalis yanhusuo Gel Plaster
Authors: Cao Guoqiong , Zhu Zilan , Yang Dingyi , Wu Wenyu , Yang Fangfang , Liu Yao , Xu Jian , Zhang Yongping 
Published: June 2024

Abstract
Corydalis yanhusuo, a traditional Chinese medicine, is widely used to treat various pains, and its active ingredients are alkaloids. This study aimed to develop a new type of transdermal gel plaster containing the extract of C. yanhusuo. Studies have shown that Fuʼcupping physical permeation-enhancing technique can promote the penetration of alkaloids and improve the efficacy of drugs. A transdermal gel plaster containing the extract of C. yanhusuo was prepared and optimized using an orthogonal experimental design. The skin permeation ability of the gel plaster was studied in vitro, while the anti-inflammatory and analgesic effects of the prepared patch alone or with Fuʼcupping physical permeation-enhancing technique were evaluated in a rat model. The formulation of a gel plaster containing C. yanhusuo extract was successfully prepared with an optimized composition consisting of glycerin (15 g), sodium polyacrylate (2 g), silicon dioxide
(0.3 g), ethanol (2 g), aluminum oxide (0.1 g), citric acid (0.05 g), the C. yanhusuo extract (3 g), and water (15 g). The cumulative transdermal permeation of dehydrocorydaline, corypalmine, tetrahydropalmatine, and corydaline in 24 h was estimated to be 569.7 ± 63.2, 74.5 ± 13.7, 82.4 ± 17.2, and 38.9 ± 8.1 µg/cm2, respectively. The in vitro diffusion of dehydrocorydaline and corydaline followed the zero-order kinetics profile, while that of corypalmine and tetrahydropalmatine followed a Higuchi equation. The prepared gel plaster significantly reduced paw swelling, downregulated inflammatory cytokines, and mitigated pain induced by mechanical or chemical stimuli. The Fuʼcupping physical permeation-enhancing technique further improved the anti-inflammatory and analgesic effects of the patch. The combined application of the Fuʼcupping physical permeation-enhancing technique and the alkaloid gel plaster may be effective against inflammation and
pain.
Social media and the rise of radical right populism in Portugal: the communicative strategies of André Ventura on X in the 2022 elections
Authors: Prior Hélder 
Published: June 2024

AbstractIn the context of media studies, the theme of populism in its relation to digital media has gained a great prominence in the last decade. The emergence of new populist political actors that use direct communication to appeal to the people without intermediaries, as well as the consolidation of digital social networks, involve interfaces between media and political processes that we intend to observe, having as object the scenario of the Portuguese Legislative Elections of 2022. Indeed, in this article we address how the populist communication of André Ventura, leader of the Portuguese radical right party, Chega, is constructed during the election campaign period. To analyze the discursive mechanisms of populism in the digital realm, we conducted a study of the corpus derived from André Ventura’s posts on the social media platform X, previously known as Twitter, during the month of January 2022, coinciding with the electoral campaign period. We sought to describe the discursive strategies present in the construction of the representation of “people”, considering the polarization “us versus them”, and examine the meanings of the world mobilized by populist communication in opposition to out-groups identified as “enemies” of the pure or authentic people. The results of our content analysis, focusing on the 143 tweets constituting Ventura’s narrative during the election period, indicate parallels with the discursive tactics of radical right populism. These include a narrative emphasizing conflict between “us and them,” a spotlight on the dysfunctions within the democratic system, the glorification of integrity in the face of political corruption, nativist discourse, and a prevailing tendency towards negative communication.
Those with constant property rights have perseverance: China’s land tenure reform promotes environment-friendly farming practices
Authors: Zheng Linyi 
Published: June 2024

AbstractFew studies have used large-scale household-level panel data to quantify the impact of land tenure reform on environment-friendly farming practices from a chemical fertiliser reduction perspective. This study attempts to fill this gap by exploring the relationship between land certification and farmers’ chemical fertiliser use. It employs nationally representative panel data from the Chinese Family Database and a propensity score matching and difference-in-difference approach. The results show that land certification significantly reduces the usage of chemical fertilisers, mainly by increasing long-term investment, promoting land renting, and inhibiting off-farm employment. This effect varies greatly between farmers with and without land certification experience before the new round of land certification, although no significant difference exists based on whether farmers have previous land adjustment experience. Furthermore, land certification also helps increase the probability of straw recycling and farm manure use. Accordingly, developing countries can combine land tenure reform with environment-friendly farming practices. Through large-scale land certification reforms, and the continuous enhancement of land tenure security, a solid foundation for a land tenure system for sustainable agricultural development can be established.