bluuebunny/crossref_metadata_2025_split huggingface dataset:
An assessment of the psychometric properties of the Coping Strategies Questionnaire – Sickle Cell Disease (CSQ-SCD) among adults in the United States
Authors: Salkar Monika , Rosenthal Meagen , Bhattacharya Kaustuv , Ramachandran Sujith , Barnard Marie , Young John , Bentley John P. 
Published: April 2024

AbstractBackgroundPrevious studies have reported conflicting factor structures of the Coping Strategies Questionnaire - Sickle Cell Disease (CSQ-SCD). This study examined the psychometric properties of the CSQ-SCD among adults with SCD in the United States.MethodsThis study implemented a cross-sectional study design with web-based self-administered surveys. Individuals with SCD were recruited via an online panel. Psychometric properties, including factorial and construct validity, and internal consistency reliability, of the CSQ-SCD were assessed.ResultsA total of 196 adults with SCD completed the survey. Confirmatory factor analysis (CFA), using maximum likelihood estimation and the 13 subscale scores as factor indicators, supported a three-factor model for the CSQ-SCD compared to a two-factor model. Model fit statistics for the three-factor model were: Chi-square [df] = 227.084 [62]; CFI = 0.817; TLI = 0.770; RMSEA [90% CI] = 0.117 [0.101–0.133]; SRMR = 0.096. All standardized factor loadings (except for the subscales isolation, resting, taking fluids, and praying and hoping) were > 0.5 and statistically significant, indicating evidence of convergent validity. Correlations between all subscales (except praying and hoping) were lower than hypothesized; however, model testing revealed that the three latent factors, active coping, affective coping, and passive adherence coping were not perfectly correlated, suggesting discriminant validity. Internal consistency reliabilities for the active coping factor (α = 0.803) and affective coping factor (α = 0.787) were satisfactory, however, reliability was inadequate for the passive adherence coping factor (α = 0.531). Given this overall pattern of results, a follow-up exploratory factor analysis (EFA) was also conducted. The new factor structure extracted by EFA supported a three-factor structure (based on the results of a parallel analysis), wherein the subscale of praying and hoping loaded on the active coping factor.ConclusionsOverall, the CSQ-SCD was found to have less than adequate psychometric validity in our sample of adults with SCD. These results provide clarification around the conflicting factor structure results reported in the literature and demonstrate a need for the future development of a SCD specific coping instrument.
The extracts of osteoblast developed from adipose-derived stem cell and its role in osteogenesis
Authors: Tangporncharoen Rattanawan , Silathapanasakul Atiruj , Tragoonlugkana Patcharapa , Pruksapong Chatchai , Tawonsawatruk Tulyapruek , Supokawej Aungkura 
Published: April 2024

AbstractCell-based therapy has become an achievable choice in regenerative medicines, particularly for musculoskeletal disorders. Adipose-derived stem cells (ASCs) are an outstanding resource because of their ability and functions. Nevertheless, the use of cells for treatment comes with difficulties in operation and safety. The immunological barrier is also a major limitation of cell therapy, which can lead to unexpected results. Cell-derived products, such as cell extracts, have gained a lot of attention to overcome these limitations. The goal of this study was to optimize the production of ASC-osteoblast extracts as well as their involvement in osteogenesis. The extracts were prepared using a freeze–thaw method with varying temperatures and durations. Overall, osteogenic-associated proteins and osteoinductive potential of the extracts prepared from the osteogenic-induced ASCs were assessed. Our results demonstrated that the freeze–thaw approach is practicable for cell extracts production, with minor differences in temperature and duration having no effect on protein concentration. The ASC-osteoblast extracts contain a significant level of essential specialized proteins that promote osteogenicity. Hence, the freeze–thaw method is applicable for extract preparation and ASC-osteoblast extracts may be beneficial as an optional facilitating biologics in bone anabolic treatment and bone regeneration.
Machine-learning-based models to predict cardiovascular risk using oculomics and clinic variables in KNHANES
Authors: Zhang Yuqi , Li Sijin , Wu Weijie , Zhao Yanqing , Han Jintao , Tong Chao , Luo Niansang , Zhang Kun 
Published: April 2024

Abstract
Background
Recent researches have found a strong correlation between the triglyceride-glucose (TyG) index or the atherogenic index of plasma (AIP) and cardiovascular disease (CVD) risk. However, there is a lack of research on non-invasive and rapid prediction of cardiovascular risk. We aimed to develop and validate a machine-learning model for predicting cardiovascular risk based on variables encompassing clinical questionnaires and oculomics.

Methods
We collected data from the Korean National Health and Nutrition Examination Survey (KNHANES). The training dataset (80% from the year 2008 to 2011 KNHANES) was used for machine learning model development, with internal validation using the remaining 20%. An external validation dataset from the year 2012 assessed the model’s predictive capacity for TyG-index or AIP in new cases. We included 32122 participants in the final dataset. Machine learning models used 25 algorithms were trained on oculomics measurements and clinical questionnaires to predict the range of TyG-index and AIP. The area under the receiver operating characteristic curve (AUC), accuracy, precision, recall, and F1 score were used to evaluate the performance of our machine learning models.

Results
Based on large-scale cohort studies, we determined TyG-index cut-off points at 8.0, 8.75 (upper one-third values), 8.93 (upper one-fourth values), and AIP cut-offs at 0.318, 0.34. Values surpassing these thresholds indicated elevated cardiovascular risk. The best-performing algorithm revealed TyG-index cut-offs at 8.0, 8.75, and 8.93 with internal validation AUCs of 0.812, 0.873, and 0.911, respectively. External validation AUCs were 0.809, 0.863, and 0.901. For AIP at 0.34, internal and external validation achieved similar AUCs of 0.849 and 0.842. Slightly lower performance was seen for the 0.318 cut-off, with AUCs of 0.844 and 0.836. Significant gender-based variations were noted for TyG-index at 8 (male AUC=0.832, female AUC=0.790) and 8.75 (male AUC=0.874, female AUC=0.862) and AIP at 0.318 (male AUC=0.853, female AUC=0.825) and 0.34 (male AUC=0.858, female AUC=0.831). Gender similarity in AUC (male AUC=0.907 versus female AUC=0.906) was observed only when the TyG-index cut-off point equals 8.93.

Conclusion
We have established a simple and effective non-invasive machine learning model that has good clinical value for predicting cardiovascular risk in the general population.
Graft failure after allogeneic hematopoietic stem cell transplantation in pediatric patients with acute leukemia: autologous reconstitution or second transplant?
Authors: Rostami Tahereh , Rostami Mohammad Reza , Mirhosseini Amir Hossein , Mohammadi Saeed , Nikbakht Mohsen , Alemi Hediyeh , Khavandgar Naghmeh , Rad Soroush , Janbabai Ghasem , Mousavi Seied Asadollah , Kiumarsi Azadeh , Kasaeian Amir 
Published: April 2024

Abstract
Background
Graft failure (GF) is a rare but serious complication after allogeneic hematopoietic stem cell transplantation (HSCT). Prevention of graft failure remains the most advisable approach as there is no clear recommendation for the best strategies for reversing this complication. Administration of growth factor, additional hematopoietic progenitor boost, or a salvage HSCT are current modalities recommended for the treatment of GF. Autologous recovery without evidence of disease relapse occurs rarely in patients with GF, and in the absence of autologous recovery, further salvage transplantation following a second conditioning regimen is a potential treatment option that offers the best chances of long-term disease-free survival. The preconditioning regimens of second HSCT have a significant impact on engraftment and outcome, however, currently there is no consensus on optimal conditioning regimen for second HSCT in patients who have developed GF. Furthermore, a second transplant from a different donor or the same donor is still a matter of debate.

Observations
We present our experience in managing pediatric patients with acute leukemia who encountered graft failure following stem cell transplantation.

Conclusions and relevance
Although a second transplantation is almost the only salvage method, we illustrate that some pediatric patients with acute leukemia who experience graft failure after an allogeneic stem cell transplant using Myeloablative conditioning (MAC) regimen may achieve long-term disease-free survival through autologous hematopoiesis recovery.
Cereal production in Africa: the threat of certain pests and weeds in a changing climate—a review
Authors: Benjamin Joshua , Idowu Oluwadamilola , Babalola Oreoluwa Khadijat , Oziegbe Emmanuel Victor , Oyedokun David Olayinka , Akinyemi Aanuoluwapo Mike , Adebayo Aminat 
Published: April 2024

AbstractCereals are the most cultivated and traded crops for food, feed, and industrial uses worldwide. Among other producing regions, Africa hosts 27% of the world's total cereal production. Like other staple crops, the production of cereals such as maize, rice, wheat, millet and sorghum in Sub-Saharan Africa is threatened by herbivorous pests and weeds leading to significant losses. The fall armyworm insect (Spodopterafrugiperda) reduces maize production by 21–53%, while the stem borers (Busseolafusca) account for 82% of all maize losses in Kenya. About 50% of yield loss in maize has been attributed to Imperatacylindrica infestations in Nigeria if not controlled. Parasitic weeds such as Strigaspp. infest over 64% of cereal-cultivated lands in Africa resulting in yield losses of up to 10–100% loss. Granivorous birds such as Queleaspp. are responsible for an average of 15–20% cereal production damage in semi-arid zones of Africa. Rodents such as the multimammate rat also pose a threat causing 48% yield losses on maize fields across Sub-Saharan Africa. With a changing climate resulting in drought and flooding, the threat of these cereal pests is likely to intensify. Hence, this review presents an elaborate overview of current pathogens whose threat to cereal production in Africa might increase due to changing climatic conditions.
Clinical, pathologic, and genomic characteristics of two pediatric glioneuronal tumors with a CLIP2::MET fusion
Authors: Chapman Nicholas , Greenwald Joshua , Suddock Jolee , Xu Dong , Markowitz Alexander , Humphrey Maeve , Cotter Jennifer A , Krieger Mark D , Hawes Debra , Ji Jianling 
Published: April 2024

AbstractIntegration of molecular data with histologic, radiologic, and clinical features is imperative for accurate diagnosis of pediatric central nervous system (CNS) tumors. Whole transcriptome RNA sequencing (RNAseq), a genome-wide and non-targeted approach, allows for the detection of novel or rare oncogenic fusion events that contribute to the tumorigenesis of a substantial portion of pediatric low- and high-grade glial and glioneuronal tumors. We present two cases of pediatric glioneuronal tumors occurring in the occipital region with a CLIP2::MET fusion detected by RNAseq. Chromosomal microarray studies revealed copy number alterations involving chromosomes 1, 7, and 22 in both tumors, with Case 2 having an interstitial deletion breakpoint in the CLIP2 gene. By methylation profiling, neither tumor had a match result, but both clustered with the low-grade glial/glioneuronal tumors in the UMAP. Histologically, in both instances, our cases displayed characteristics of a low-grade tumor, notably the absence of mitotic activity, low Ki-67 labeling index and the lack of necrosis and microvascular proliferation. Glial and neuronal markers were positive for both tumors. Clinically, both patients achieved clinical stability post-tumor resection and remain under regular surveillance imaging without adjuvant therapy at the last follow-up, 6 months and 3 years, respectively. This is the first case report demonstrating the presence of a CLIP2::MET fusion in two pediatric low-grade glioneuronal tumors (GNT). Conservative clinical management may be considered for patients with GNT and CLIP2:MET fusion in the context of histologically low-grade features.
Scientific analysis of two compound eye beads unearthed in Hejia Village, Zhouling
Authors: Li Jingyu , Sun Feng , Zhang Yanglizheng , Ha Wenhui , Yan Haihong , Zhai Congwen 
Published: April 2024

Abstract
The glass compound eye bead is the exquisite embodiment of the glassmaking technology of ancient craftsmen, and is an example of the cultural exchange between China and the West during the Warring States Period. This study takes two dots and mesh beads with seriously weathered surfaces excavated from Hejia village, Zhouling as the research object. Micromorphology, X-ray fluorescence spectroscopy (XRF), microscopic laser Raman spectroscopy and X-ray photoelectron spectroscopy (XPS) were respectively used to determine the chemical elements, valence states and compositions of the glass matrix and its weathering products. In this study, the valence state analysis of group-d elements is used to reveal the electron transition mode and explain the colour formation reason of blue-black glass matrix. In terms of weathering products, in addition to the identification of common cerusite [PbCO3] and barite [BaSO4], the study also found the blue weathering product alforsite [Ba5(PO4)3Cl] for the first time. The study also makes reasonable assumptions about the reasons for their appearance. The X-ray photoelectron spectroscopy (XPS) used in this study has positive significance for the study of the formation mechanism of glass colour. The scientific and technological analysis data of these two glass beads provide basic data for the related research of the lead-barium glass system produced in China, and also provide a certain scientific basis for the related protection research.
Evaluation of the use of a clinical practice guideline for external apical root resorption among orthodontists
Authors: van Doornik Sebastiaan P. , Pijnenburg Marlotte B. M. , Janssen Krista I. , Ren Yijin , Kuijpers-Jagtman Anne Marie 
Published: April 2024

Abstract
Background
External apical root resorption (EARR) is a frequently observed adverse event in patients undergoing fixed appliance therapy. Assessing the patients’ risk during treatment is important, as certain factors are assumed to be associated with an increased likelihood of occurrence. However, their predictive value remains limited, making evidence-based clinical decision-making challenging for orthodontists. To address this issue, the Dutch Association of Orthodontists (NvVO) developed a clinical practice guideline (CPG) for EARR in accordance with the AGREE II instrument (Appraisal of Guidelines for Research and Evaluation II) in 2018. The aim of this study is to get insight into the actual utilization and the practical implementation of the guideline among orthodontists. The hypothesis to be tested was that after its introduction, clinical practice for EARR has changed towards the recommendations in the CPG.

Objective
To investigate the use of the 2018 clinical practice guidelines for EARR among orthodontists 3 years after its introduction.

Methods
A questionnaire using a 7-point Likert scale was developed concerning four domains of EARR described in the guideline. The questionnaire was piloted, finalised, and then distributed digitally among Dutch orthodontists. REDCap was used for data collection, starting with an invitation email in June 2021, followed by two reminders. Effect was tested by the Mann–Whitney U test, and the influence of demographic variables was analysed.

Results
Questionnaires were sent out to all 275 and completed by 133 (response rate 48%); N = 59 females and N = 73 males were included; 81% had their training in the Netherlands, 89% had ≥ 6 years of work experience, and 89% worked in private orthodontic practice. One hundred thirty orthodontists (98.5%) reported changes in clinical practice. The biggest positive change in clinical behaviour regarding EARR occurred if EARR was diagnosed during treatment. Sex, clinical experience, country of specialist training, and working environment of the respondents did not affect clinical practices regarding EARR.

Conclusions
This questionnaire demonstrated that, 3 years after introduction of the guideline, orthodontists improved their self-reported clinical practices to a more standardised management of root resorption. None of the demographic predictors had a significant effect on the results.
Investigating item complexity as a source of cross-national DIF in TIMSS math and science
Authors: Huang Qi , Bolt Daniel M. , Lyu Weicong 
Published: April 2024

Abstract
Background
Large scale international assessments depend on invariance of measurement across countries. An important consideration when observing cross-national differential item functioning (DIF) is whether the DIF actually reflects a source of bias, or might instead be a methodological artifact reflecting item response theory (IRT) model misspecification. Determining the validity of the source of DIF has implications for how it is handled in practice.

Method
We demonstrate a form of sensitivity analysis that can point to model misspecification induced by item complexity as a possible cause of DIF, and show how such a cause of DIF might be accommodated through attempts to generalize the IRT model for the studied item(s) in psychometrically and psychologically plausible ways.

Results
In both simulated illustrations and empirical data from TIMSS 2011 and TIMSS 2019 4th and 8th Grade Math and Science, we have found that using a form of proposed IRT model generalization can substantially reduce DIF when IRT model misspecification is at least a partial cause of the observed DIF.

Conclusions
By demonstrating item complexity as a possible valid source of DIF and showing the effectiveness of the proposed approach, we recommend additional attention toward model generalizations as a means of addressing and/or understanding DIF.
Study on the use of Imazalil to continuous cropping obstacle of Ganoderma lucidum caused by Xylogone ganodermophthora
Authors: Huang Qiuru , Lu Qi , Cao Fenghua , Li Xiaomin , Wu Xiaoping , Sun Huijuan , Zhang Junli , Fu Junsheng 
Published: April 2024

Abstract
Background
The Xylogone ganodermophthora is a pathogenic bacterium that poses a significant challenge to the continuous cultivation of the Ganoderma lucidum fungus. This study aims to identify and investigate specific agents for the effective prevention and control of X. ganodermophthora, establishing a theoretical foundation for overcoming this persistent challenge in G. lucidum cultivation.

Results
Using different G. lucidum soil as materials to study the presence of X. ganodermophthora in the soil. Additionally, the plate confrontation test was employed to investigate the impact of X. ganodermophthora on G. lucidum growth. The impact of physical factors and antibacterial agents on pathogenic bacteria was successfully carried out, with a further exploration of the effectiveness of field control. PCR amplification experiment and sequencing analysis verified that X. ganodermophthora existed in G. lucidum continuous cropping obstacle soil. This pathogenic bacteria has a significant inhibitory effect on the growth of G. lucidum, with an inhibition rate of up to 52.23%. High temperature, low temperature, light and other physical factors have no obvious inhibitory effect on this pathogen. Further investigation revealed that specific drugs, such as low concentrations (10 μL/mL) of Acticide DB20 and Imazalil, could effectively inhibit X. ganodermophthora growth in G. lucidum. Among them, Imazalil has a notable inhibitory effect on the growth of X. ganodermophthora.

Conclusions
Indoor toxicity test and field control results showed that Imazalil could effectively control the growth of pathogen X. ganodermophthora in G. lucidum continuous cropping obstacle, and promote the growth of G. lucidum.

Graphical Abstract
Curative resection via right hemicolectomy and regional lymph node dissection for colonic adenomatous polyposis of unknown etiology with adenocarcinomas localized in the right side of the colon: a case report
Authors: Aoyama Shu , Inoue Akira , Kagawa Yoshinori , Komori Takamichi , Ozato Yuki , Nishizawa Yujiro , Sugimoto Tomoki , Komatsu Hisateru , Hirota Masashi , Miyazaki Yasuhiro , Tomokuni Akira , Motoori Masaaki , Fushimi Hiroaki , Yamamoto Gou , Akagi Kiwamu , Iwase Kazuhiro , Fujitani Kazumasa 
Published: April 2024

Abstract
Background
APC and MUTYH are both well-known colorectal polyposis causative genes. However, 30–50% of colorectal adenomatous polyposis cases are classified as colonic adenomatous polyposis of unknown etiology and lack identifiable pathogenic variants. Although guidelines recommend total proctocolectomy for colonic adenomatous polyposis of unknown etiology with over 100 adenomas, evidence is lacking. This study presents a unique case of localized colonic adenomatous polyposis of unknown etiology with multiple adenocarcinomas, treated with hemicolectomy and regional lymph node dissection.

Case presentation
The patient was a 72-year-old woman whose colonoscopy revealed numerous polyps and two adenocarcinomas localized in the right side of the colon, with no lesions in the left side. The patient had no family history of polyposis or colorectal cancer. No extracolonic lesions, enlarged lymph nodes, or distant metastases were found. Considering the patient’s age and lesion localization, laparoscopic right hemicolectomy with regional lymph node dissection was performed. Histopathological diagnosis revealed three adenocarcinoma lesions with no lymph node metastasis. The most advanced pathological stage was T2N0M0 Stage I (UICC 8th edition). The patient was alive 5 years postoperatively, without recurrence of cancer or polyposis in the remaining colon and rectum. To diagnose hereditary colorectal cancer/polyposis, a germline multigene panel testing for APC, EPCAM, MBD4, MLH1, MLH3, MSH2, MSH3, MSH6, MUTYH, NTHL1, PMS2, POLD1, POLE, and TP53 was performed using DNA extracted from blood samples: however, no pathogenic variant was detected. Therefore, the patient was diagnosed with colonic adenomatous polyposis of unknown etiology.

Conclusions
In this rare case, colonic adenomatous polyposis of unknown etiology, with numerous adenomatous polyps and multiple adenocarcinomas localized in the right side of the colon, was successfully treated with right hemicolectomy and regional lymph node dissection. Despite genetic analysis, no causative germline variants were identified. Segmental colectomy according to the distribution of polyps might be a curative approach.