bluuebunny/crossref_metadata_2025_split huggingface dataset:
Myo-inositol as a main metabolite in overwintering flies: seasonal metabolomic profiles and cold stress tolerance in a northern drosophilid fly
Authors: Vesala Laura , Salminen Tiina S. , Koštál Vladimir , Zahradníčková Helena , Hoikkala Anneli 
Published: July 2012

SUMMARYCoping with seasonal changes in temperature is an important factor underlying the ability of insects to survive over the harsh winter conditions in the northern temperate zone, and only a few drosophilids have been able to colonize sub-polar habitats. Information on their winter physiology is needed as it may shed light on the adaptive mechanisms of overwintering when compared with abundant data on the thermal physiology of more southern species, such as Drosophila melanogaster. Here we report the first seasonal metabolite analysis in a Drosophila species. We traced changes in the cold tolerance and metabolomic profiles in adult Drosophila montana flies that were exposed to thermoperiods and photoperiods similar to changes in environmental conditions of their natural habitat in northern Finland. The cold tolerance of diapausing flies increased noticeably towards the onset of winter; their chill coma recovery times showed a seasonal minimum between late autumn and early spring, whereas their survival after cold exposure remained high until late spring. The flies had already moderately accumulated glucose, trehalose and proline in autumn, but the single largest change occurred in myo-inositol concentrations. This increased up to 400-fold during the winter and peaked at 147 nmol mg−1 fresh mass, which is among the largest reported accumulations of this compound in insects.
Evaporative water loss, relative water economy and evaporative partitioning of a heterothermic marsupial, the monito del monte (Dromiciops gliroides)
Authors: Withers Philip C. , Cooper Christine E. , Nespolo Roberto F. 
Published: July 2012

SUMMARYWe examine here evaporative water loss, economy and partitioning at ambient temperatures from 14 to 33°C for the monito del monte (Dromiciops gliroides), a microbiotheriid marsupial found only in temperate rainforests of Chile. The monito's standard evaporative water loss (2.58 mg g−1 h−1 at 30°C) was typical for a marsupial of its body mass and phylogenetic position. Evaporative water loss was independent of air temperature below thermoneutrality, but enhanced evaporative water loss and hyperthermia were the primary thermal responses above the thermoneutral zone. Non-invasive partitioning of total evaporative water loss indicated that respiratory loss accounted for 59–77% of the total, with no change in respiratory loss with ambient temperature, but a small change in cutaneous loss below thermoneutrality and an increase in cutaneous loss in and above thermoneutrality. Relative water economy (metabolic water production/evaporative water loss) increased at low ambient temperatures, with a point of relative water economy of 15.4°C. Thermolability had little effect on relative water economy, but conferred substantial energy savings at low ambient temperatures. Torpor reduced total evaporative water loss to as little as 21% of normothermic values, but relative water economy during torpor was poor even at low ambient temperatures because of the relatively greater reduction in metabolic water production than in evaporative water loss. The poor water economy of the monito during torpor suggests that negative water balance may explain why hibernators periodically arouse to normothermia, to obtain water by drinking or via an improved water economy.
Encephalic photoreception and phototactic response in the troglobiont Somalian blind cavefish Phreatichthys andruzzii
Authors: Tarttelin Emma E. , Frigato Elena , Bellingham James , Di Rosa Viviana , Berti Roberto , Foulkes Nicholas S. , Lucas Robert J. , Bertolucci Cristiano 
Published: July 2012

SUMMARY
Many physiological and behavioural responses to changes in environmental lighting conditions are mediated by extraocular photoreceptors. Here we investigate encephalic photoreception in Phreatichthys andruzzii, a typical cave-dwelling fish showing an extreme phenotype with complete anophthalmy and a reduction in size of associated brain structures. We firstly identified two P. andruzzii photopigments, orthologues of rod opsin and exo-rod opsin. In vitro, both opsins serve as light-absorbing photopigments with λmax around 500 nm when reconstituted with an A1 chromophore. When corrected for the summed absorption from the skin and skull, the spectral sensitivity profiles shifted to longer wavelengths (rod opsin: 521 nm; exo-rod opsin: 520 nm). We next explored the involvement of both opsins in the negative phototaxis reported for this species. A comparison of the spectral sensitivity of the photophobic response with the putative A2 absorbance spectra corrected for skin/skull absorbance indicates that the A2 versions of either or both of these pigments could explain the observed behavioural spectral sensitivity.
Trafficking of bdelloid rotifer late embryogenesis abundant proteins
Authors: Tripathi Rashmi , Boschetti Chiara , McGee Brian , Tunnacliffe Alan 
Published: July 2012

SUMMARY
The bdelloid rotifer Adineta ricciae is an asexual microinvertebrate that can survive desiccation by entering an ametabolic state known as anhydrobiosis. Two late embryogenesis abundant (LEA) proteins, ArLEA1A and ArLEA1B, have been hypothesized to contribute to desiccation tolerance in these organisms, since in vitro assays suggest that ArLEA1A and ArLEA1B stabilize desiccation-sensitive proteins and membranes, respectively. To examine their functions in vivo, it is important to analyse the cellular distribution of the bdelloid LEA proteins. Bioinformatics predicted their translocation into the endoplasmic reticulum (ER) via an N-terminal ER translocation signal and persistence in the same compartment via a variant C-terminal retention signal sequence ATEL. We assessed the localization of LEA proteins in bdelloids and in a mammalian cell model. The function of the N-terminal sequence of ArLEA1A and ArLEA1B in mediating ER translocation was verified, but our data showed that, unlike classical ER-retention signals, ATEL allows progression from the ER to the Golgi and limited secretion of the proteins into the extracellular medium. These results suggest that the N-terminal ER translocation signal and C-terminal ATEL sequence act together to regulate the distribution of rotifer LEA proteins within intracellular vesicular compartments, as well as the extracellular space. We speculate that this mechanism allows a small number of LEA proteins to offer protection to a large number of desiccation-sensitive molecules and structures both inside and outside cells in the bdelloid rotifer.
Electronic individual identification of zebrafish using radio frequency identification (RFID) microtags
Authors: Cousin Xavier , Daouk Tarek , Péan Samuel , Lyphout Laura , Schwartz Marie-Elise , Bégout Marie-Laure 
Published: July 2012

SUMMARYAlthough individual electronic tagging using passive integrated acoustic (PIT) tags is established, it is mainly for fish &gt;60 mm in length and is unsuitable for fish of &lt;30 mm, like zebrafish. We used radio frequency identification (RFID) microtags (1 mm in diameter and 6 mm in length, with a mass of ~10 mg) to individually identify juvenile zebrafish (length 16–42 mm, mass 138–776 mg) for the first time, and studied the effects of intracoelomic implantation on fish survival and microtag loss, growth, spawning and exploratory behaviour. After 5.5 months, both high survival (82%) and low microtag loss (11%) were achieved. The smallest surviving fish weighed 178 mg, and success in microtag reading was 73% for the size class 350–450 mg (26 mm). Greater success was achieved when fish were larger at the time of tagging but no negative effects on growth were observed for any size class and some tagged fish spawned. No significant differences in behavioural responses could be detected between tagged fish and untagged controls after 2 months. Overall, the results suggest that the tagging method is highly suitable for fish as small as zebrafish juveniles. We think this method will provide significant advances for researchers of the ever-growing fish model community and more generally for all small-fish users. Tagging is essential when one needs to identify fish (e.g. particular genotypes with no external cue), to run longitudinal monitoring of individual biological traits (e.g. growth) or to repeat assays with the same individual at discrete points in time (e.g. behaviour studies). Such a method will find applications in physiology, genetics, behaviour and (eco)toxicology fields.
Intracellular Na+, K+ and Cl− activities in Acheta domesticus Malpighian tubules and the response to a diuretic kinin neuropeptide
Authors: Coast Geoffrey M. 
Published: July 2012

SUMMARY
The mechanism of primary urine production and the activity of a diuretic kinin, Achdo-KII, were investigated in Malpighian tubules of Acheta domesticus by measuring intracellular Na+, K+ and Cl− activities, basolateral membrane voltage (Vb), fluid secretion and transepithelial ion transport. Calculated electrochemical gradients for K+ and Cl− across the basolateral membrane show they are actively transported into principal cells, and basolateral Ba2+-sensitive K+ channels do not contribute to net transepithelial K+ transport and fluid secretion. A basolateral Cl− conductance was revealed after the blockade of K+ channels with Ba2+, and a current carried by the passive outward movement of Cl− accounts for the hyperpolarization of Vb in response to Ba2+. Ion uptake via Na+/K+/2Cl− cotransport, driven by the inwardly directed Na+ electrochemical gradient, is thermodynamically feasible, and is consistent with the actions of bumetanide, which reduces fluid secretion and both Na+ and K+ transport. The Na+ gradient is maintained by its extrusion across the apical membrane and by a basolateral ouabain-sensitive Na+/K+-ATPase. Achdo-KII has no significant effect on the intracellular ion activities or Vb. Electrochemical gradients across the apical membrane were estimated from previously published values for the levels of Na+, K+ and Cl− in the secreted fluid. The electrochemical gradient for Cl− favours passive movement into the lumen, but falls towards zero after stimulation by Achdo-KII. This coincides with a twofold increase in Cl− transport, which is attributed to the opening of an apical Cl− conductance, which depolarises the apical membrane voltage.
Interlocking of chelae is a key factor for dominance hierarchy formation in crayfish
Authors: Ueno Ryusuke , Nagayama Toshiki 
Published: July 2012

SUMMARYWe characterized the role of chelae during agonistic encounters of the crayfish Procambarus clarkii. Physical asymmetries in body length, body mass and chelae size were directly related to dominance hierarchy formation. More than 80% of winning crayfish had longer body and chelae lengths, and winners were usually heavier in body mass, even if their differences were less than 3% compared with losing opponents. In mismatched pairings, large crayfish with short chelae were beaten by small crayfish with large chelae. Three physical elements of body length, body mass and chelae length equally affected the outcome of agonistic bouts. Chelae restriction, in which chelae were tightly closed using rubber bands, affected the outcome of agonistic bouts between large and small crayfish. Before chelae restriction, large crayfish won in all pairings. Following chelae restriction for at least 30 days from the first encounters, the winning rate of large crayfish that were previous winners decreased significantly in the second encounters against the same opponents that were previous losers. The handicap of chelae restriction significantly prolonged the time to formation of the winner–loser relationship. Individual fights escalated during agonistic bouts between large crayfish with one chela restricted and small crayfish with intact chelae, whereas the number of fights increased but the duration of individual fights did not increase in large crayfish with both chelae restricted. Furthermore, when the chelae of both large and small crayfish were disabled, the dominance order was frequently not formed during 30 min of agonistic bouts. Preventing chelae from interlocking prevented escalation of agonistic bouts. We show that interlocking of chelae acted as a key factor for the formation of dominance hierarchy.
Effect of fenofibrate treatment for hyperlipidaemia on serum creatinine and cystatin C
Authors: Ncube Vusumuzi , Starkey Bryan , Wang Tim 
Published: July 2012

Background The increase in creatinine in patients on fibrate therapy is well-recognized, but its mechanism is not clearly understood. A study by Hottelart et al. suggested that fibrate-induced creatininaemia was due to the effect of fibrates on creatinine metabolism as opposed to a decline in renal function. To address this hypothesis, we have monitored renal function in a group of hyperlipidaemic patients before commencing fibrate treatment and after three months of therapy. Methods We studied 12 subjects (10 men, 2 women), median age 43.5 y (range 33–70 y). Serum creatinine, cystatin C, creatine kinase and fasting lipids were measured. Results We observed statistically significant increases in concentrations of serum creatinine ( P < 0.005) and cystatin C( P < 0.01). Concentrations of both analytes increased in 10 (83.3%) of the patients. In these patients, the median increases were 15.1% (range 5.5–23.2%) for creatinine and 9.9% (range 1.1–26.1%) for cystatin C. Conclusions These results suggest that the decrease in estimated glomerular filtration rate, observed in patients undergoing fibrate therapy, is a genuine effect on kidney function rather than a change in creatinine metabolism as previously postulated, since the rises in serum creatinine concentration were reflected by rises in cystatin C, an independent marker of renal function.
Lurasidone: An Atypical Antipsychotic for Schizophrenia
Authors: Risbood Vineeta , Lee Jennifer R , Roche-Desilets Jennifer , Fuller Matthew A 
Published: July 2012

OBJECTIVE: To provide a clinical overview of the antipsychotic lurasidone. DATA SOURCES: Articles were identified by searching the MEDLINE, PubMed, Cochrane Library, and EBSCO databases (through February 2012) using the key word lurasidone. The manufacturer provided information on unpublished Phase 2 and 3 trials. The Clinicaltrials.gov database was reviewed for the status of ongoing and upcoming trials. STUDY SELECTION AND DATA EXTRACTION: All clinical trials lasting longer than 3 weeks and published in the English language were selected for review. Additional documentation, including the product dossier, package insert, and poster presentations supplied by the publisher, was also evaluated. DATA SYNTHESIS: Lurasidone hydrochloride is an atypical antipsychotic that is approved for the treatment of schizophrenia. It is under investigation for treatment of bipolar I disorder. It should be administered with food, is pregnancy category B, is contraindicated for coadministration with strong CYP3A4 inducers and inhibitors, and requires dose adjustments with certain medications and in renal and hepatic impairment. Like other atypical antipsychotics, lurasidone possesses dopamine D2 and serotonin 5-HT2A antagonism but exhibits little affinity for histamine H1, α1-adrenergic, or cholinergic M1 receptors. Additionally, it is a potent 5-HT7 antagonist, which may impact depression and cognition. Phase 3 trial results revealed that 40-80 mg administered once daily resulted in statistically significant improvements in schizophrenia symptomatology compared with placebo. Lurasidone's rate of metabolic adverse events is low relative to other atypical antipsychotics; however, this is offset by dose-dependent increases in somnolence, akathisia, and parkinsonism. CONCLUSIONS: Lurasidone has shown efficacy when compared to placebo in acute schizophrenia. Full characterization of the adverse effect profile and cognitive and affective benefits requires publication of trials with longer durations.
Discrepancies in Medication Information for the Primary Care Physician and the Geriatric Patient at Discharge
Authors: Cornu Pieter , Steurbaut Stephane , Leysen Tinne , De Baere Eva , Ligneel Claudine , Mets Tony , Dupont Alain G 
Published: July 2012

BACKGROUND: Medication discrepancies in discharge medication lists can lead to medication errors and adverse drug events following discharge. OBJECTIVE: To determine the incidence and type of discrepancies between the discharge letter for the primary care physician and the patient discharge medication list as well as identify possible patient-related determinants for experiencing discrepancies. METHODS: A retrospective, single-center, cohort study of patients discharged from the acute geriatric department of a Belgian university hospital between September 2009 and April 2010 was performed. Medications listed in the discharge letter for the primary care physician were compared with those in the patient discharge medication list. Based on the clinical pharmacist–acquired medication list at hospital admission and the medications administered during hospitalization, we determined for every discrepancy whether the medication listed in the discharge letter or the patient discharge medication list was correct. RESULTS: One hundred eighty-nine discharged patients (mean [SD] age 83.9 [5.7] years, 64.0% female) were included in the study. Almost half of these patients (90; 47.6%) had 1 or more discrepancies in medication information at discharge. The discharge letters were often more complete and accurate than the patient discharge medication lists. The most common discrepancies were omission of a brand name in the patient discharge medication list and omission of a drug in the discharge letter. Increasing numbers of drugs in the discharge medication list (OR 1.19; 95% CI 1.07 to 1.32; p = 0.001) and discharge letter (OR 1.18; 95% CI 1.07 to 1.32; p = 0.001) were associated with a higher risk for discrepancies. CONCLUSIONS: Discrepancies between the patient discharge medication list and the medication information in the discharge letter for the primary care physician occur frequently. This may be an important source of medication errors, as confusion and uncertainty about the correct discharge medications can originate from these discrepancies. Increasing numbers of drugs involve a higher risk for discrepancies. Medication reconciliation between both lists is warranted to avoid medication errors.
REVIEWS
Authors: Salmon Andy , Wheale Nigel 
Published: July 2012

THE TRUTH WILL TRIP YOU UP MATTHEW SWEENEY THE NIGHT POST, SALT MODERN POETS SERIES, (2009) London: Salt, 2010, 144pp., ISBN: 9781844714841(pbk), £12.99 BEING HUMAN, NEIL ASTLEY (ED), (2009) Newcastle-upon-Tyne: Bloodaxe, 2011, 512pp., ISBN: 1 85224 809 2(pbK), £12.00
COHERENT,
HUMAN READING CONTEMPORARY POETRY: POETS AND POETRY SINCE 1990, CONTEXTS IN LITERATURE SERIES, IAN BRINTON (ED.), ADRIAN BARLOW (SERIES ED.), (2009) Cambridge: Cambridge University Press, 128pp., ISBN 9780521712484 (pbk), £8.95 A MANNER OF UTTERANCE: THE POETRY OF J.H. PRYNNE, IAN BRINTON
(ED.), (2009) Bristol: Shearsman Books, 188pp., ISBN 9781848610439 (pbk), £12.95/$20.00, ISBN 9781848640422 (hbk), £29.95/$45.00 THE SALT COMPANION TO JOHN JAMES, SIMON PERRIL (ED.), (2010) London: Salt Publishing, 280pp., ISBN 9781876857967 (pbk), £14.99/$21.95
SUB SONGS, J.H. PRYNNE, (2010) London: Barque Press, 24pp., ISBN 9781903488744 (pbk), £10.00
Effect of Surface Treatments on Microtensile Bond Strength of Repaired Aged Silorane Resin Composite
Authors: Palasuk J , Platt JA , Cho SD , Levon JA , Brown DT , Hovijitra ST 
Published: July 2012

SUMMARY
Objective: This laboratory study compared the repaired microtensile bond strengths of aged silorane resin composite using different surface treatments and either silorane or methacrylate resin composite. Methods: One hundred eight silorane resin composite blocks (Filtek LS) were fabricated and aged by thermocycling between 8°C and 48°C (5000 cycles). A control (solid resin composite) and four surface treatment groups (no treatment, acid treatment, aluminum oxide sandblasting, and diamond bur abrasion) were tested (N=12 blocks, 108 beams/group). Each treatment group was randomly divided in half and repaired with either silorane resin composite (LS adhesive) or methacrylate resin composite (Filtek Z250/Single Bond Plus). After 24 hours in 37°C distilled water, microtensile bond strength testing was performed using a non-trimming technique. Surface topography after surface treatment was analyzed using scanning electron microscopy (SEM). Failure mode was examined using optical microscopy (50×). Results: Weibull-distribution survival analysis revealed that aluminum oxide sandblasting followed by silorane or methacrylate resin composite and acid treatment with methacrylate resin composite provided insignificant differences from the control (p&gt;0.05). All other groups were significantly lower than the control. Failure was primarily adhesive in all groups. Conclusion: Aluminum oxide sandblasting produced microtensile bond strength not different from the cohesive strength of silorane resin composite. After aluminum oxide sandblasting, aged silorane resin composite can be repaired with either silorane resin composite with LS system adhesive or methacrylate resin composite with methacrylate dental adhesive.
Ultrasound Guided Alcohol Ablation of Morton's Neuroma
Authors: Musson Rachel E. , Sawhney Jasdev S. , Lamb Leslie , Wilkinson Antony , Obaid Haron 
Published: July 2012

Background: Morton's neuroma is a common cause of metatarsalgia. This study evaluated the efficacy of ultrasound guided alcohol injection as a treatment for this condition. Method: Data from 87 treatment courses were included in this study with a mean follow of 14.3 months. Results: Technical success was 100%. One patient developed symptoms consistent with an allergic reaction to the injection and one patient declined further injection because of periprocedural pain. Partial or total treatment response was achieved in 66%, with 32% of patients having complete resolution of pain. The median visual analogue score (VAS) decreased from 8 pre-procedure to 4 post-procedure ( p < 0.0001). Procedural success was greater in patients under 55 years old and in those with solitary neuromas. Seventeen patients (20%) went on to have surgery due to continuing pain. Conclusion: Ultrasound guided alcohol ablation for the treatment of Morton's neuroma was a safe procedure that significantly reduced pain and may offer an alternative therapy to surgery. Level of Evidence: IV; Retrospective Case Series