bluuebunny/crossref_metadata_2025_split huggingface dataset:
A viral small interfering RNA-host plant mRNA pathway modulates virus-induced drought tolerance by enhancing autophagy
Authors: Wu Xinyang , Chen Shuting , Zhang Zixin , Zhou Weixin , Sun Ting , Ning Kang , Xu Min , Ke Xubo , Xu Pei 
Published: May 2024

Abstract
Virus-induced drought tolerance presents a fascinating facet of biotic–abiotic interaction in plants, yet its molecular intricacies remain unclear. Our study shows that cowpea mild mottle virus (CPMMV) infection enhances drought tolerance in common bean (Phaseolus vulgaris) plants through a virus-derived small interfering RNA (vsiRNA)-activated autophagy pathway. Specifically, a 21 nt vsiRNA originating from the CPMMV Triple Gene Block1 (TGB1) gene targeted the 5′ untranslated region (UTR) of the host Teosinte branched 1, Cycloidea, Proliferating Cell Factor (TCP) transcription factor gene PvTCP2, independent of the known role of TGB1 as an RNA silencing suppressor. This targeting attenuated the expression of PvTCP2, which encodes a transcriptional repressor, and in turn upregulated the core autophagy-related gene (ATG) PvATG8c, leading to activated autophagy activity surpassing the level induced by drought or CPMMV infection alone. The downstream EARLY RESPONSIVE TO DEHYDRATION (ERD) effector PvERD15 is a homologue of Arabidopsis thaliana AtERD15, which positively regulates stomatal aperture. PvERD15 was degraded in PvATG8c-mediated autophagy. Therefore, we establish a TGB1-PvTCP2-PvATG8c-PvERD15 module as a trans-kingdom fine-tuning mechanism that contributes to virus-induced drought tolerance in plant–drought–virus interactions.
Accurate and rapid molecular subgrouping of high-grade glioma via deep learning-assisted label-free fiber-optic Raman spectroscopy
Authors: Liu Chang , Wang Jiejun , Shen Jianghao , Chen Xun , Ji Nan , Yue Shuhua 
Published: May 2024

Abstract
Molecular genetics is highly related with prognosis of high-grade glioma. Accordingly, the latest WHO guideline recommends that molecular subgroups of the genes, including IDH, 1p/19q, MGMT, TERT, EGFR, Chromosome 7/10, CDKN2A/B, need to be detected to better classify glioma and guide surgery and treatment. Unfortunately, there is no preoperative or intraoperative technology available for accurate and comprehensive molecular subgrouping of glioma. Here, we develop a deep learning-assisted fiber-optic Raman diagnostic platform for accurate and rapid molecular subgrouping of high-grade glioma. Specifically, a total of 2,354 fingerprint Raman spectra was obtained from 743 tissue sites (astrocytoma: 151; oligodendroglioma: 150; glioblastoma (GBM): 442) of 44 high-grade glioma patients. The convolutional neural networks (ResNet) model was then established and optimized for molecular subgrouping. The mean area under receiver operating characteristic curves (AUC) for identifying the molecular subgroups of high-grade glioma reached 0.904, with mean sensitivity of 83.3%, mean specificity of 85.0%, mean accuracy of 83.3%, and mean time expense of 10.6 s. The diagnosis performance using ResNet model was shown to be superior to PCA-SVM and UMAP models, suggesting that high dimensional information from Raman spectra would be helpful. In addition, for the molecular subgroups of GBM, the mean AUC reached 0.932, with mean sensitivity of 87.8%, mean specificity of 83.6%, and mean accuracy of 84.1%. Furthermore, according to saliency maps, the specific Raman features corresponding to tumor-associated biomolecules (e.g. nucleic acid, tyrosine, tryptophan, cholesteryl ester, fatty acid, and collagen) were found to contribute to the accurate molecular subgrouping. Collectively, this study opens up new opportunities for accurate and rapid molecular subgrouping of high-grade glioma, which would assist optimal surgical resection and instant post-operative decision-making.
Human mesenchymal stromal cells ameliorate cisplatin-induced acute and chronic kidney injury via TSG-6
Authors: Tang Ming , Shen Linguo , Tang Maozhi , Liu Ling , Rao Zhengsheng , Wang Zhilin , Wang Yadi , Yin Supei , Li Shujing , Xu Guilian , Zhang Keqin 
Published: May 2024

Abstract
Cisplatin is widely used in tumor chemotherapy, but nephrotoxicity is an unavoidable side effect of cisplatin. Several studies have demonstrated that mesenchymal stromal cells (MSCs) ameliorate cisplatin-induced kidney injury, but the underlying mechanisms are unknown. In this study, the cisplatin-induced kidney injury mouse model was established by subjecting a single intraperitoneal injection with cisplatin. One hour before cisplatin injection, the mice received human bone marrow MSCs (hBM-MSCs) with or without siRNA-transfection, recombinant human tumor necrosis factor-α-stimulated gene/protein 6 (rhTSG-6), or PBS through the tail vein. In addition, cisplatin-stimulated HK-2 cells were treated with hBM-MSCs or rhTSG-6. Human BM-MSCs treatment remarkably ameliorated cisplatin-induced acute and chronic kidney injury, as evidenced by significant reductions in serum creatinine (Scr), blood urea nitrogen, tubular injury, collagen deposition, α-smooth muscle actin accumulation, as well as inflammatory responses, and by remarkable increased anti-inflammatory factor expression and Treg cells infiltration in renal tissues. Furthermore, we found that only a few hBM-MSCs engrafted into damaged kidney and that the level of human TSG-6 in the serum of mice increased significantly following hBM-MSCs administration. Moreover, hBM-MSCs significantly increased the viability of damaged HK-2 cells and decreased the levels of inflammatory cytokines in the culture supernatant. However, the knockdown of the TSG-6 gene in hBM-MSCs significantly attenuated their beneficial effects in vivo and in vitro. On the contrary, treated with rhTSG-6 achieved similar beneficial effects of hBM-MSCs. Our results indicate that systemic administration of hBM-MSCs alleviates cisplatin-induced acute and chronic kidney injury in part by paracrine TSG-6 secretion.
Updating, correcting, and calibrating the narrative about premature ejaculation
Authors: Rowland David L , Tamas Stella , Hevesi Krisztina 
Published: May 2024

Abstract

Introduction
The narrative surrounding premature ejaculation (PE) has developed and solidified over the past 25 years. Unfortunately, portions of that narrative are outdated and do not reflect more recent conceptualizations or empirical findings regarding this disorder.

Objective
In this review we sought to identify existing narratives about PE in need of updating and to provide revised narratives based on the recent research literature.

Method
Five PE narratives in need of revision were identified, including: the prevalence of PE, age-related differences in PE prevalence, a validated ejaculation latency (EL) for diagnosing PE, differences between lifelong and acquired PE subtypes, and the application of PE definitions beyond penile-vaginal intercourse. Extensive literature searches provided information supporting both the original narrative and the need for a revised narrative based on both consideration of more recent studies and reinterpretation of studies conducted since the establishment of the original narratives.

Results
For each selected topic, the prevailing narrative based on the extant literature was first presented, followed by discussion of accumulating evidence that challenges the existing narrative. Each section ends with a suggested revised PE narrative. In 2 instances, the revised narrative required significant corrections (eg, PE prevalence, validated EL for diagnosing PE); in 2 instances, it expanded on the existing narrative (eg, PE subtype differences, inclusion of partnered sexual activities beyond penile-vaginal intercourse); and in 2 other instances, it backed off prior conclusions that have since required rethinking (eg, age-related changes in PE, PE subtype differences). Finally, a brief review of the 3-pronged criteria for PE (EL, ejaculatory control, and bother/distress) is presented and discussed.

Conclusion
This review reiterates the dynamic state of research on PE and demonstrates the need for and value of ongoing research that not only addresses new issues surrounding this dysfunction but also challenges and revises some of the existing narratives about PE.
A taxonomy of didactic roles of dynamic visualization in animated mathematics videos
Authors: ten Voorde Abe , Piroi Margherita , Bos Rogier 
Published: May 2024

Abstract
In recent years, a multitude of innovative educational videos focusing on mathematics and employing dynamic visualization techniques have been published on YouTube. From a sample of videos, we develop a taxonomy of didactical roles of dynamic visuals and supporting animation techniques. The taxonomy is constructed through an open coding approach, involving an iterative process of defining, analysing and refining the codes. Additionally, we conducted an interview with a video creator about the intended roles of dynamic visuals employed in his animations. The taxonomy encompasses the following categories of roles: connect objects, (co)vary objects, dynamic concept visualization, dynamic process visualization, symbol sense, connect to reality and generalization. These roles are implemented through animation techniques like moving and morphing. The nomenclature of these roles suggests their affiliation with various mathematics education research perspectives, including covariational thinking, concept–process duality, symbol sense and conversion between registers. The taxonomy serves as a foundational framework for further research into the learning outcome and effectiveness of dynamic visualization in educational mathematics videos.
The endocannabinoid anandamide activates pro‐resolving pathways in human primary macrophages by engaging both CB2 and GPR18 receptors
Authors: Leuti Alessandro , Fava Marina , Forte Giulia , Pellegrini Niccolò , Oddi Sergio , Scipioni Lucia , Gomez Esteban A. , Dalli Jesmond , Maccarrone Mauro 
Published: May 2024

AbstractResolution of inflammation is the cellular and molecular process that protects from widespread and uncontrolled inflammation and restores tissue function in the aftermath of acute immune events. This process is orchestrated by specialized pro‐resolving mediators (SPM), a class of bioactive lipids able to reduce immune activation and promote removal of tissue debris and apoptotic cells by macrophages. Although SPMs are the lipid class that has been best studied for its role in facilitating the resolution of self‐limited inflammation, a number of other lipid signals, including endocannabinoids, also exert protective immunomodulatory effects on immune cells, including macrophages. These observations suggest that endocannabinoids may also display pro‐resolving actions. Interestingly, the endocannabinoid anandamide (AEA) is not only known to bind canonical type 1 and type 2 cannabinoid receptors (CB1 and CB2) but also to engage SPM‐binding receptors such as GPR18. This suggests that AEA may also contribute to the governing of resolution processes. In order to interrogate this hypothesis, we investigated the ability of AEA to induce pro‐resolving responses by classically‐activated primary human monocyte‐derived macrophages (MoDM). We found that AEA, at nanomolar concentration, enhances efferocytosis in MoDMs in a CB2‐ and GPR18‐dependent manner. Using lipid mediator profiling, we also observed that AEA modulates SPM profiles in these cells, including levels of resolvin (Rv)D1, RvD6, maresin (MaR)2, and RvE1 in a CB2‐dependent manner. AEA treatment also modulated the gene expression of SPM enzymes involved in both the formation and further metabolism of SPM such as 5‐lipoxygenase and 15‐Prostaglandin dehydrogenase. Our findings show, for the first time, a direct effect of AEA on the regulation of pro‐resolving pathways in human macrophages. They also provide new insights into the complex interactions between different lipid pathways in activation of pro‐resolving responses contributing to the reestablishment of homeostasis in the aftermath of acute inflammation.
“If You Plant That Seed, It Will Grow”: A Qualitative Study to Improve Linkage to Care Among Patients With Methamphetamine Use Disorder in Emergency Department Settings
Authors: Rinehart Deborah J. , Duarte Karina G. , Gilbert Aiden , Al-Tayyib Alia , Camfield Katherine , Simpson Scott A. 
Published: May 2024

Objectives
Methamphetamine addiction is a serious and difficult-to-treat disorder. Existing treatment options are limited, and patient perspectives on effective strategies are lacking. Emergency departments (EDs) may be a critical entry point for individuals with methamphetamine use disorder (MUD) to be identified and linked to treatment. We aimed to understand patients’ perspectives regarding their methamphetamine use and related ED experiences and how to improve linkage to substance treatment.

Methods
Between July and November 2022, semistructured qualitative interviews were conducted with adult patients with MUD in an urban safety-net healthcare setting in Denver, Colorado. Interviews were recorded, summarized, and analyzed using the Rapid Assessment Process.

Results
During the interviews, 18 patients shared their experiences. Participants described feeling stigmatized and experiencing a lack of communication from ED staff during their visit. Additionally, participants shared the perception that ED staff often did not take their health concerns seriously once substance use was identified. Participants were uncertain about overdose risk and felt that their psychiatric symptoms complicated treatment. Referrals to treatment were lacking, and participants supported a care navigation intervention that incorporates elements of contingency management. Participants also shared the importance of ED staff recognizing their social needs and being empathetic, trauma-informed, and flexible to meet patients where they are regardless of their readiness to seek treatment.

Conclusions
Treatment options and entry points for individuals with MUD are currently limited. The patient perspectives described here are helpful in developing services to support, engage, and link individuals to MUD services after discharge from ED services.
Bupropion-disguised chest pain presenting in a middle-aged male: a case report and review of literature
Authors: Ahmed Hasaan , Ismayl Mahmoud , Palicherla Anirudh , Selim Mohammad , Aboeata Ahmed 
Published: May 2024

Introduction and importance:
Chest pain is one of the most prevalent complaints amongst individuals presenting in healthcare settings, encompassing a broad spectrum of etiologies. Work-up for chest pain often focuses on excluding life-threatening conditions before the consideration of atypical causes.

Case presentation:
A 47-year-old male with a past medical history of tobacco use and depression presented with persistent left-sided chest pain. Vitals on arrival were notable for mild hypertension. Two consecutive high-sensitivity troponins were unremarkable. The electrocardiogram showed sinus rhythm with no ischemic changes. Due to the atypical presentation of chest pain, the patient’s home medications were reviewed, and his bupropion was discontinued due to concern for medication-induced chest pain. The patient was discharged and presented 2 days for follow-up endorsing complete resolution of his chest pain.

Clinical discussion:
Prior investigations have shown bupropion to be associated with chest pain, with resolution noted after discontinuation. The etiology of chest pain is likely sympathomimetic, as bupropion has been shown to exhibit positive inotropic effects on myocardial tissue, propagated by catecholamine release.

Conclusion:
Patients taking bupropion may present with atypical chest pain. Medication discontinuation may be beneficial in alleviating symptoms.
Temporal bone mucocele with extension to mastoid in a 60-year-old male: a case report and literature review
Authors: Shah Ujjwal Kumar , Muskan Vitasta , Dahal Alok , Malla Justin Jung , Lamichhane Samiksha , Shah Krishnandan 
Published: May 2024

Introduction:
Mucoceles, characterized by cystic lesions with sterile mucus accumulation, mostly occur in the paranasal but rarely affect the temporal-mastoid bone. The pathogenesis involves obstruction of natural drainage or cyst enlargement. Here, the authors present a rare case of temporal-mastoid mucocele with temporal hypertrophy.

Case presentation:
A 60-year-old male presented with progressive painless right temporal region swelling associated with diminished hearing, headache, fever and ear symptoms. Audiometry indicated a profound hearing loss in the right ear. Computed tomography (CT) of the right temporal bone and MRI brain revealed a large, destructive lesion involving the right temporal mastoid suggesting temporal-mastoid mucocele. Surgical excision of the mucocele and mastoid exploration were performed. Postoperative recovery was uneventful, with significant improvement in symptoms.

Clinical discussion:
Temporal bone mucoceles are rare, with few cases reported in the literature. Their etiology remains unclear, although chronic otitis media may be associated in some cases. Clinical presentations may vary, ranging from asymptomatic to symptoms such as serous otitis media, retroauricular edema, or cholesteatoma. Expansion of the mucocele cavity can lead to bone erosion or remodeling, potentially causing hearing loss. Differentials should include cholesteatoma, histiocytosis, and cholesterol granuloma. Imaging such as temporal bone CT and MRI are crucial for diagnosing, with characteristic findings on T2W images helping in differentiation. Surgical treatment, such as mastoidectomy with marsupialization or complete lesion removal, is indicated to prevent complications and reduce the risk of recurrence.

Conclusion:
This study reports a unique case of concomitant temporal-mastoid mucocele with involvement of organs of hearing with temporal bone hypertrophy.
Primary gastrointestinal stromal tumor of the liver: a case report
Authors: Pham Anh Tuan , Pham Anh The , Truong Cuong Manh , Nguyen Thang Huu , Trinh Phuong Huy 
Published: May 2024

Introduction and importance:
Primary gastrointestinal stromal tumors of the liver are exceedingly rare entities, presenting diagnostic and therapeutic challenges. The authors present a case of a 64-year-old male with a primary gastrointestinal stromal tumor (GIST) of the liver, emphasizing the importance of comprehensive diagnostic evaluation and multidisciplinary management in such uncommon cases.

Case presentation:
The patient presented with persistent hypochondriac pain, leading to the discovery of a hepatic mass. Diagnostic work-ups, including imaging studies and biopsy, confirmed the diagnosis of primary GIST in the liver. Following thorough multidisciplinary consultation, the patient underwent right anterior segmentectomy of the liver, performed by our experienced surgeon. Postoperative pathology confirmed the diagnosis of GIST, and the patient was advised to use adjuvant imatinib.

Clinical discussion:
Primary GISTs of the liver pose diagnostic challenges due to their rarity and varied clinical presentations. Imaging modalities, immunohistochemistry, and molecular genotyping are crucial in accurate diagnosis and treatment planning. Surgical resection remains the cornerstone of treatment for localized GISTs, with adjuvant therapy considered based on recurrence risk factors and molecular characteristics.

Conclusion:
This case highlights the need for multidisciplinary consultation in managing primary GISTs of the liver. Accurate diagnosis, surgical expertise, and personalized adjuvant therapy are crucial for better patient outcomes. Further research is necessary to enhance our understanding of prognostic factors and treatment strategies for these rare tumors.
Stevens–Johnson syndrome associated with pancytopenia: a case report
Authors: Prasai Parikshit , Shakya Selika , Singh Avilasha , Manandhar Sarbagya , Adhikari Bibek 
Published: May 2024

Introduction and importance:
Stevens–Johnson syndrome (SJS) is a rare and unusual hypersensitivity reaction to certain drugs like allopurinol, commonly used for treating gout. SJS is recognized by extensive necrosis and detachment of skin and mucus membranes. Pancytopenia, characterized by decreased levels of red blood cells, white blood cells and platelets, is an exceedingly rare occurrence in the rare disorder SJS.

Case presentation:
The authors present a 61-year-old male who exhibited symptoms of fever and rash for 5 days accompanied by pancytopenia and liver injury.

Clinical discussion:
The abdomen and bilateral lower extremities exhibited several well-defined dusky-colored hyperpigmented macular lesions. Initially, these lesions were small, tender, erythematous, and raised, later transitioning to a dark red. Multiple distinct ulcerations were present on the lips and buccal cavity. Additionally, there was denudation of the skin with bleeding observed between the toes of both legs. The causality was assessed as a definite adverse drug reaction according to the Naranjo and ALDEN algorithm. The patient received treatment consisting of intravenous steroid along with prophylactics antibiotics. The individual’s pancytopenia was resolved without requiring any blood cells or plasma or platelet concentrate transfusion.

Conclusion:
The exact pathophysiology of SJS associated with pancytopenia has not yet been fully elucidated. The authors’ study hypothesized that the cause of pancytopenia in SJS could be either the direct cytotoxicity of drugs or immune-mediated damage to the bone marrow cells. Additional studies are necessary to establish the precise pathophysiology of the condition. Moreover, our study also indicates that pancytopenia can resolve in SJS without the need for blood cells or plasma or platelet concentrate transfusion. Once more, further studies are required to establish precise management strategies for managing SJS associated with pancytopenia.