bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serotype and genomic diversity of dengue virus during the 2023 outbreak in Pakistan reveals the circulation of genotype III of DENV‐1 and cosmopolitan genotype of DENV‐2
Authors: Jamal Zunera , Haider Syed Adnan , Hakim Rabia , Humayun Fahad , Farooq Muhammad Umar , Ammar Muhammad , Afrough Babak , Inamdar Leena , Salman Muhammad , Umair Massab 
Published: June 2024

AbstractDengue, a mosquito‐borne viral disease, poses a significant public health challenge in Pakistan, with a significant outbreak in 2023, prompting our investigation into the serotype and genomic diversity of the dengue virus (DENV). NS‐1 positive blood samples from 153 patients were referred to the National Institute of Health, Pakistan, between July and October 2023. Among these, 98 (64.1%) tested positive using multiplex real‐time PCR, with higher prevalence among males (65.8%) and individuals aged 31‐40. Serotyping revealed DENV‐1 as the predominant serotype (84.7%), followed by DENV‐2 (15.3%). Whole‐genome sequencing of 18 samples (DENV‐1 = 17, DENV‐2 = 01) showed that DENV‐1 (genotype III) samples were closely related (>99%) to Pakistan outbreak samples (2022), and approx. > 98% with USA (2022), Singapore and China (2016), Bangladesh (2017), and Pakistan (2019). The DENV‐2 sequence (cosmopolitan genotype; clade IVA) shared genetic similarity with Pakistan outbreak sequences (2022), approx. > 99% with China and Singapore (2018−2019) and showed divergence from Pakistan sequences (2008−2013). No coinfection with dengue serotypes or other viruses were observed. Comparisons with previous DENV‐1 sequences highlighted genetic variations affecting viral replication efficiency (NS2B:K55R) and infectivity (E:M272T). These findings contribute to dengue epidemiology understanding and underscore the importance of ongoing genomic surveillance for future outbreak responses in Pakistan.
Proof of infectivity of hepatitis E virus particles from the ejaculate of chronically infected patients
Authors: Schemmerer Mathias , Bock Hans H. , Schattenberg Jörn M. , Huber Samuel , Polywka Susanne , Mader Maria , Lohse Ansgar W. , Todt Daniel , Steinmann Eike , Wenzel Jürgen J. , Horvatits Thomas , Pischke Sven 
Published: June 2024

AbstractRecently, hepatitis E virus (HEV, Paslahepevirus balayani) particles were detected for the first time in the ejaculate of two chronically infected patients. Since then, we have been able to detect HEV in ejaculate in five further patients, and thus in a total of seven out of nine (78%) chronically infected men (age 36–67 years, median 56 years). In five patients, the HEV RNA concentration was more than 100‐fold higher compared to the serum, while in two patients, the viral load was more than 10‐fold lower. However, it has remained unclear whether viral particles shed in the ejaculate were infectious, as a previous cell culture model had failed to demonstrate the infectivity. In the current study, we employed an optimized HEV cell culture system based on overconfluent PLC/PRF/5 cells to investigate the infectivity of HEV particles from ejaculate and other body fluids. With this approach, we were able to show for the first time that HEV particles in the ejaculate from several patients were infectious. HEV replicated to high viral loads of 1e9 HEV RNA copies per ml. This indicates that HEV‐positive ejaculate could bear a risk of infection for sexual partners.
Submerged imprint of glacier dynamics in the NW sector of Lago Viedma (southern Patagonia, Argentina)
Authors: Restelli F. B. , Lozano J. G. , Bran D. M. , Bunicontro S. , Lodolo E. , Tassone A. A. , Vilas J. F. 
Published: June 2024

ABSTRACTLago Viedma is a proglacial lake into which the Viedma Glacier flows from the Southern Patagonian Ice Field. This glacier has rapidly lost mass in recent times, and its deglacial history is reflected in the lake's subsurface. New high‐resolution multi‐channel seismic profiles acquired in the northwestern sector of the lake have allowed us to reconstruct the bathymetry of the area and identify several small sub‐basins, which have a maximum depth of 240 m in this sector. Four seismic facies have been recognized, separated by erosional unconformities, reflecting the depositional conditions in this sector of the basin during different Quaternary sedimentation phases. There is a transitional phase from ice‐contacted deposits to subglacial deposits, probably associated with a subglacial fan, and finally a phase of lacustrine sedimentation. In addition, three depositional stages were identified within the lacustrine deposits, indicating a different sedimentary input, and the morphologies observed at the lake bottom suggest the existence of at least ten stagnations of the glacial margin. This study helps to improve the history of the retreat of the Viedma Glacier in this area during the Pleistocene/Holocene and provides a basis for further geophysical measurements aimed at mapping this remote lacustrine environment.
Determination of the binding constants and ionic mobilities of diquat complexes with randomly sulfated cyclodextrins by affinity capillary electrophoresis
Authors: Bílek Jan , Koval Dušan , Šolínová Veronika , Talele Harish R. , Severa Lukáš , Gutiérrez Paul E. Reyes , Teplý Filip , Kašička Václav 
Published: June 2024

The enantiomers of diquats (DQs), a new class of functional organic molecules, were recently separated by capillary electrophoresis (CE) with high resolution up to 11.4 within 5–7 min using randomly sulfated α‐, β‐, and γ‐cyclodextrins (CDs) as chiral selectors. These results indicated strong interactions between dicationic diquats and multiply negatively charged sulfated CDs (S‐CDs). However, the binding strength of these interactions was not quantified. For that reason, in this study, affinity CE was applied for the determination of the binding constants and ionic mobilities of the complexes of DQ P‐ and M‐enantiomers with CD chiral selectors in an aqueous medium. The non‐covalent interactions of 10 pairs of DQ enantiomers with the above CDs were investigated in a background electrolyte (BGE) composed of 22 mM NaOH, 35 mM H3PO4, pH 2.5, and 0.0–1.0 mM concentrations of CDs. The average apparent binding constant and the average actual ionic mobility of the DQ‐CD complexes were determined by nonlinear regression analysis of the dependence of the effective mobility of DQ enantiomers on the concentration of CDs in the BGE. The complexes were found to be relatively strong with the averaged apparent binding constants in the range 13 600–547 400 L/mol.
Effects of popliteal nerve blocks on postoperative pain management in fibula‐free flap patients for head and neck cancer reconstruction
Authors: Gemmiti Amanda , Audlin Jason , Debick Nadia , Proper Heidi , Yeturu Prabhat , Walsh Ronald , Ryan Jesse , Samudre Susan 
Published: June 2024

AbstractObjectiveTo determine if performing popliteal nerve blocks preoperatively in patients undergoing fibula‐free flap surgery for head and neck cancer reconstruction decreases subjective pain scores decreases narcotic usage, and improves mobility in the acute postoperative time period when compared to alternative pain control regimens.MethodsA retrospective review of the medical records of patients who underwent fibula‐free flap reconstruction for head and neck malignancy at SUNY Upstate Medical University during the time period from 2015 to 2022 was performed. Collected data consisted of patient demographics and clinical characteristics, postoperative pain management modalities, reported pain scores, postoperative narcotic usage, length of hospital stay, and days until out of bed without personal assistance.ResultsA total of 40 patients were included in the study. The average reported pain score was reduced in the nerve block group compared to the control group (1.7 vs. 4.0, p‐value = .003). Similarly, the average maximum reported pain score was also lower in patients who received a nerve block (3.4 vs. 6.9, p‐value = .002). None of the patients who received popliteal nerve blocks required pain control with parenteral narcotics postoperatively, whereas 82.9% of patients without a nerve block did. Patients who received a popliteal nerve block consumed an average of 103.5 MME, whereas those who did not receive a block consumed an average of 523.0 MME. No statistically significant difference was found between the groups regarding time from surgery until transfer without personal assistance or length of hospital stay.ConclusionPopliteal nerve blocks can reduce postoperative pain in patients undergoing fibula‐free flap reconstruction for head and neck cancer.
Impact of human papillomavirus status on survival in patients with oral cancer
Authors: Tangthongkum Manupol , Phisalmongkhon Suwapat , Leelasawatsuk Peesit , Supanimitjaroenporn Pasawat , Kirtsreesakul Virat , Tantipisit Jarukit 
Published: June 2024

AbstractObjectivesTo examine the association between the human papillomavirus (HPV) infection and overall survival rate in patients with oral cancer.MethodsThis retrospective cohort study examined HPV status in 454 patients who were diagnosed with oral squamous cell carcinoma (OSCC) using the records of patients who underwent an initial treatment for OSCC between 2012 and 2021 at our institution as retrieved from the Cancer Registry database. The survival rates of the HPV‐positive and HPV‐negative groups were assessed and compared, and independent factors associated with survival were analyzed using multivariate Cox regression models.ResultsOf the 454 patients with OSCC included in this study, 73 were excluded for invalid HPV tests. Of the remaining patients, 39 and 342 patients were categorized into HPV‐positive and HPV‐negative groups, respectively. The prevalence of HPV‐positive in the patients with OSCC was 10.2% (95% confidence interval 7.2%–13.2%). The 3‐year overall survival rates were 56.2% and 53.9% in the HPV‐positive and HPV‐negative groups, respectively. The 3‐year disease‐specific survival rates in the HPV‐positive and HPV‐negative groups were 60.2% and 56.9%, respectively. The survival differences were not statistically significant. HPV‐positive status was not a significant predictor of overall survival in the multivariable Cox regression analyses (p = 0.728).ConclusionThe prevalence of HPV‐positivity among patients with OSCC in the study was 10.2%. No association was found between HPV‐positive status and 3‐year overall survival in patients with oral cancer.Level of evidenceLevel 3.
Co‐creative gift systems within digital platforms
Authors: Ciampa Isabella , de Valck Kristine 
Published: June 2024

AbstractThis study explores co‐creative gift systems within digital platforms. We define co‐creative gift systems as systems where consumers actively engage in content creation and co‐creation, disseminating original artistic digital gifts (i.e., content such as novels, videos, music) on digital platforms and engaging in collaborative exchanges that shape the work in progress. Using a qualitative methodology, including netnography and guided introspections, we analyze 3 years of netnographic data on Wattpad, a platform for developing and publishing written fiction. Our findings reveal the building of varying relational ties within the gift system, from casual interactions to deep connections that foster communities of practice and fandoms, and fluid role transitions between givers and recipients. These interactions and transitions impact the development of the digital gift through editorial support, narrative co‐creation, and independent reviews. This study contributes to the literature on gift giving by emphasizing the role of co‐creative gift systems in enhancing digital community interactions and content co‐creation. Future research directions are suggested to further explore co‐creative gift systems across various digital platforms and their implications for consumer research and digital content production.
Feeling younger and acting greener: The impact of subjective age on sustainable consumption
Authors: Lee Daniel Chaein , Kim Jungkeun 
Published: June 2024

AbstractIt is widely believed that younger generations are at the forefront of sustainability efforts. Given the significance of fostering greater consumer participation in sustainable consumption, this perception prompts an intriguing question: If consumers associate sustainability with younger individuals, can simply instilling a sense of youthfulness increase their engagement in sustainable consumption, regardless of their actual age? Building upon previous research on subjective age—that is, how old one feels—and consumer identity, we propose that consumers will gravitate towards sustainable products when they feel younger than older. This is because such products align well with their perceived age identity, creating a perception of fit. The findings across three studies provide converging evidence that simply making consumers feel younger can result in an increased preference for sustainable products. Study 1 demonstrated that feeling younger increased the selection of more sustainable products over less sustainable ones. Study 2 showed that this effect is mediated by the perceived fit. Study 3 further supported the underlying mechanism by revealing that the focal effect was mitigated when the association between sustainability and younger age was weakened. Study 4 replicated the focal effect in a consequential choice setting. Finally, Study 5 confirmed the proposed effect by using trait subjective age as a predictor.
Hyperexpression of tumor necrosis factor receptor 2 inhibits differentiation of myeloid‐derived suppressor cells by instigating apolarity during ageing
Authors: Wang Ming , Han Yijie , Yao Xiaohan , Duan Xixi , Wan Jiajia , Lou Xiaohan , Yan Yan , Zheng Peiguo , Wang Fazhan , Zhu Linyu , Ni Chen , Pan Zhenzhen , Wang Zihao , Chen Lin , Wang Zhaoqing , Qin Zhihai 
Published: June 2024

AbstractDuring the ageing process, TNF‐α can promote the expansion of myeloid‐derived suppressor cells (MDSCs). However, it remains unclear which receptor(s) of TNF‐α are involved in and how they modulate this process. Here, we report that TNFR2 hyperexpression induced by either TNF‐α or IL‐6, two proinflammatory factors of senescence‐associated secretory phenotype (SASP), causes cellular apolarity and differentiation inhibition in aged MDSCs. Ex vivo overexpression of TNFR2 in young MDSCs inhibited their polarity and differentiation, whereas in vivo depletion of Tnfr2 in aged MDSCs promotes their differentiation. Consequently, the age‐dependent increase of TNFR2 versus unaltered TNFR1 expression in aged MDSCs significantly shifts the balance of TNF‐α signaling toward the TNFR2–JNK axis, which accounts for JNK‐induced impairment of cell polarity and differentiation failure of aged MDSCs. Consistently, inhibiting JNK attenuates apolarity and partially restores the differentiation capacity of aged MDSCs, suggesting that upregulated TNFR2/JNK signaling is a key factor limiting MDSC differentiation during organismal ageing. Therefore, abnormal hyperexpression of TNFR2 represents a general mechanism by which extrinsic SASP signals disrupt intrinsic cell polarity behavior, thereby arresting mature differentiation of MDSCs with ageing, suggesting that TNFR2 could be a potential therapeutic target for intervention of ageing through rejuvenation of aged MDSCs.
A novel missense variant located within the zinc finger domain of the GLI3 gene was identified in a Vietnamese pedigree with index finger polydactyly
Authors: Nguyen Thy Ngoc , Tran Giang Son , Hoang Hai Duc , Nguyen Long Giang 
Published: June 2024

AbstractBackgroundPolydactyly, particularly of the index finger, remains an intriguing anomaly for which no specific gene or locus has been definitively linked to this phenotype. In this study, we conducted an investigation of a three‐generation family displaying index finger polydactyly.MethodsExome sequencing was conducted on the patient, with a filtration to identify potential causal variation. Validation of the obtained variant was conducted by Sanger sequencing, encompassing all family members.ResultsExome analysis uncovered a novel heterozygous missense variant (c.1482A>T; p.Gln494His) at the zinc finger DNA‐binding domain of the GLI3 protein within the proband and all affected family members. Remarkably, the variant was absent in unaffected individuals within the pedigree, underscoring its association with the polydactyly phenotype. Computational analyses revealed that GLI3 p.Gln494His impacts a residue that is highly conserved across species.ConclusionThe GLI3 zinc finger DNA‐binding region is an essential part of the Sonic hedgehog signaling pathway, orchestrating crucial aspects of embryonic development through the regulation of target gene expression. This novel finding not only contributes valuable insights into the molecular pathways governing polydactyly during embryonic development but also has the potential to enhance diagnostic and screening capabilities for this condition in clinical settings.
Transformation of hexagonal boron nitride nanocomposite underfill in microelectronics packaging
Authors: Razgaleh S. A. , Aravamudhan Shyam 
Published: June 2024

AbstractIn recent years, hexagonal boron nitride (h‐BN) has gained attention for its potential applications in thermal management and high‐temperature uses. To evaluate the environmental impact of employing h‐BN nanocomposites in microelectronics packaging for thermal management, a comprehensive assessment was conducted. This assessment is particularly significant due to the nature of nanoparticles and the importance of mitigating potential environmental toxicity. Electronic waste incineration, compared to e‐waste recycling, carries a higher environmental burden. Building on previous research, this project explores the feasibility of h‐BN nanocomposites in microelectronic packaging for thermal management. The study focuses on the physiochemical transformations of h‐BN nanocomposite underfills during incineration, considering the influence of particle size and concentration on these transformations. Various analytical techniques, including SEM, XRD, FTIR spectroscopy, and TEM, were employed to identify and characterize the particles throughout their life cycle. By delving into physiochemical transformations and investigating the effects of particle size and concentration, this research enhances our understanding of h‐BN nanocomposites, aiding informed decision‐making for environmentally sustainable use in microelectronics packaging.Highlights
h‐BN nanoparticles remained inert throughout their life cycle.
Underfill characterization showed minimal physiochemical changes in h‐BN.
h‐BN is a promising thermal transfer candidate in microelectronics.
Study on the expansion of nitrile rubber in high‐pressure hydrogen gas atmospheres: Calculations and experiments
Authors: Lei Xiaozhen , Zheng Bin , Xue Dongxu 
Published: June 2024

AbstractHydrogen with small molecule size under high pressure can permeate most materials, being a challenge for rubber sealing materials used in hydrogen storage. The volume expansion of rubber materials due to high‐pressure hydrogen is an important indicator of their degradation and failure. Here, both microscopic calculations and experimental investigations of the nitrile rubber were conducted to evaluate the relation between the structure features and the expansion performance. Three factors, the acrylonitrile amount, the chain length, and the crosslinking degree, were considered to impact on the expansion of the nitrile rubber. Current study indicates that the increasing of acrylonitrile amount can result in the reduction of the volume expansion, owing to the preferential interactions between hydrogen and the butadiene group of nitrile rubber. Both of the chain length and the crosslinking degree are positively proportional to the chain elongation and then the volume of the nitrile rubber. Especially, the amount of acrylonitrile was found to be a key factor for evaluating the expansion of nitrile rubber under high‐pressure hydrogen. This study performs microscopic calculations and macroscopic experiments to investigate the expansion of nitrile rubber, which is expected to be used in designing the sealing rubber material for high‐pressure hydrogen storage applications.Highlights
Acrylonitrile determines the expansion performance of NBR within high‐pressure hydrogen.
Short chain and low crosslinking density suppressed the expansion of NBR.
Evaluating the expansion of nitrile rubber exposed to high‐pressure hydrogen.
Colloidal and thermal properties of poly(hydroxyethyl caprolactone acrylate‐co‐methyl methacrylate)‐g‐pullulan latex obtained by seeded semi‐batch emulsion polymerization
Authors: Santos Samuel A. , Romão Eduardo G. M. , Nahra Lara R. , Guerrini Lila M. , Oliveira Maurício P. 
Published: June 2024

AbstractIn this study, the influence that the monomer composition of hydroxyethyl caprolactone acrylate/methyl methacrylate (HECLA/MMA) has on the properties of the latex films, including contact angle, thermogravimetric analysis, and mechanical properties was evaluated. The polymerization process to obtain small particle sizes, good colloidal stability, and polymer films was optimized through the use of pullulan (Pull) and different ratios of HECLA and MMA. The results revealed a substantial impact of the monomer composition and polymerization processes on the particle size and latex film properties. The use of seed latex obtained in situ reduces particle size and particle size distribution. Additionally, it was observed that the contact angle of the polymer films increased as the concentration of HECLA increased. Furthermore, the water absorption of the polymer films exhibited a decreasing trend after reaching an 80/20 monomer composition of HECLA/MMA. An increase in HECLA contents in the recipe showed a reduction in tensile strength, elastic modulus, hardness, and elongation at break. Conversely, higher HECLA contents contributed to improving the thermal properties of the polymer films. Finally, the optimal ratio of HECLA/MMA to improve the thermal degradation and tensile mechanical properties of the polymer film was 50/50 wt%. The results found in this work allow additional data for producing poly(HECLA‐co‐MMA)‐g‐Pull latexes by manipulating the ratios of HECLA and MMA, which can create new potential applications in various industrial settings for a wide range of polysaccharides.Highlights
Poly(HECLA‐co‐MMA)‐g‐Pull with different ratios of HECLA/MMA were successfully obtained.
The use of Pull after molar mass reduction had a significant effect on colloidal stability.
A reduction in tensile strength and elongation at break was obtained by increasing the amount of HECLA.
Thermal stability of the latex films showed a direct relationship with the content of HECLA.
Poly(HECLA‐co‐MMA)‐g‐Pull latexes can be used in agricultural applications.