bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effects of Cold and Heat Stress on the Chemistry and Cell Structure of Peanut Seeds1
Authors: Singleton J. A. , Pattee H. E. 
Published: June 2010

Abstract
Frost damage and/or cold stress to crops in the United States runs more than $1 billion per year. In North Carolina during 1983, about 30,000 t of peanuts valued at $16 million were diverted from the edible market due to freeze damage. It is difficult to distinguish if a lot of peanut seed has been exposed to cold or heat stress because in both cases anaerobic respiration predominates. This research was undertaken to distinguish between cold and heat stresses at the cellular level on peanut seed by using dynamic headspace analysis, specific conductivity of the seed leachate, ion chromatography for cations and organic acids, ninhydrin detection for amino acids, and light microscopic techniques. Acetaldehyde, ethanol, and ethyl acetate are produced by both stress conditions. Two isomers of 2,3-butanediol and short-chain acids were identified from cold-stressed seed. These isomers were not found in heat-stressed peanut tissues and can be used to differentiate between the two environmental stresses. Specific conductivity of the leachate was higher from cold-stressed seed than from normal or heat-stressed seed, due to a higher efflux of potassium and acetic acid from the cells. Plasmolysis of the cold-stressed seed cells was consistently observed. Aleurone grains appeared to be larger, more dispersed, and tended to migrate towards the cell wall in the heat-treated samples, whereas the aleurone grains in the cold-stressed samples were smaller and more concentrated at the center of the cell. Irregular cell shape was common to both stresses.
A Diverging Belt Screen for Farmer Stock Peanuts1
Authors: Blankenship P. D. , Dowell F. E. 
Published: June 2010

Abstract
Separating farmer stock (FS) peanut materials into more than two diameters with conventional belt screens requires more than one screen with different spacings between belts. A new type of belt screen (diverging belt) was developed which separates materials into six sizes. The effects of varying the diverging belt screen operational parameters on separating farmer stock peanuts (cv. Florunner), loose shelled kernels (LSK), and foreign materials (FM) were evaluated with three replications. The operational parameters for two of the replicates were six consecutive positions along the deck length (30.5, 20.3, 50.8, 50.8, 50.8 cm and riding the entire deck length); six belt speeds (101.6, 111.8, 122.1, 132.3, 142.6, and 152.4 cm/sec); and six material feed rates (MatFR) (0.9, 1.8, 2.7, 3.6, 4.5, and 5.4 Mg/hr). Deck lengths and belt speeds were the same for the third replicate but MatFRs of 1.4, 2.7, 4.1, 5.4, 6.8, and 8.2 Mg/hr were used to evaluate performance of the screen at higher flow rates. Materials were collected into six compartments according to where they fell through the screen. The amount of materials falling through the screen deck varied directly with screen deck length, belt speed, and material feed rate. Screen deck length had the greatest effect on the amount of materials falling through. The diverging belt screen provides an alternative for multi-separation of FS peanut materials based on diameter.
Regeneration of Plants from Apical Meristem Tips and Nodal Segments of Arachis pintoi
Authors: Rey H. Y. , Mroginski L. A. 
Published: June 2010

Abstract
The in vitro regeneration potential of shoot apical tips (2 to 3 mm in length), meristems (0.3 to 0.5 mm in length), and nodal segments (4 to 7 mm long with an axillary bud) of diploid (2n = 2x = 20) and triploid (2n = 3x = 30) cytotypes of Arachis pintoi was evaluated. Explants were cultured on MS medium supplemented with different concentrations and combinations of naphthaleneacetic acid (NAA) and benzyladenine (BA). In one experiment the effect of gibberellic acid was tested. The cultures were done in liquid and solid media. Plant regeneration can be readily achieved from all explants in one step of 30 d culture on MS + 0.01 mg/L each of NAA and BA or two steps consisting of 1) shoots regeneration through culture of explants on MS + 0.01 mg/L each of NAA and BA, and 2) induction of rooting in regenerated shoots by reculture on MS + 0.01 mg/L NAA. The plantlets were successfully transferred to pots in a greenhouse.
A Note on the Accuracy and Variability of Grading and Marketing High Moisture Farmer Stock Peanuts
Authors: Lamb M. C. , Blankenship P. D. , Whitaker T. B. , Butts C. L. 
Published: June 2010

Abstract
Previous research has shown that the farmer stock grade, lot weight, and market value could be accurately determined at kernel moisture contents greater than 10.5% without negative impact on either the producer or purchaser. In the 1998 and 1999 crop years, 686 farmer stock lots consisting of runner, Virginia, and Spanish types were graded and weighed at high moisture content (HMC), cured, and graded and weighed at low moisture content (LMC). The results indicated that LMC grade, lot weight, and lot value could be accurately predicted from HMC grade, lot weight, and lot value for individual farmer stock lots. However, the research did not address variability between HMC and LMC grade, weight, and values. In crop year 2001, a study was conducted in Georgia on runner-type peanuts to address variability in HMC and LMC grade, weight, and values. As farmer stock lots entered the buying point each lot was graded and weighed six times at HMC. The prediction equations estimated from the 1998 and 1999 studies were applied to the HMC values to obtain predicted grades, lot weights, and lot values. The lot was cured and graded and weighed six times at LMC and compared to the six predicted grades, lot weights, and lots values. Thirty-two farmer stock lots were included in the study. There were no significant differences in mean grade, lot weight, or lot value between the predicted and actual LMC values. Sound mature kernels and sound splits (SMKSS) differed by 0.07%. Mean lot weight differed by 7.7 kg (0.13%). Mean lot value differed by $20.11 (0.53%). Variability between predicted and actual SMKSS, lot weight, and lot value was not significantly different.
Histochemical Localization of Amino Acids and Sugars in Peanut Cotyledons for Light Microscopy1
Authors: Young C. T. , Pattee H. E. , Schadel W. E. , Sanders T. H. 
Published: June 2010

Abstract
Light microscopy staining methods for amino acids and sugars were adapted for glutaraldehyde vapor-fixed, freeze dried, plastic embedded tissue of peanut cotyledons (Arachis hypogaea L.). These methods revealed the locale of amino acids and sugars, which are regarded as precursors of roasted peanut flavor compounds. Amino acids were localized in the protein bodies and cytoplasmic network by Coomassie Brilliant Blue G-250, Orange G, and diacetylbenzene. Sugars were localized in the protein bodies and cytoplasmic network by Alcian Blue - PAS procedure and an adaptation of the Okamoto method for sugars. Localization of the amino acids and sugars provide further evidence to the importance of the protein bodies in the cotyledon in the production of roasted peanut flavor.
Genotype × Seeding Rate Interaction among TSWV-Resistant, Runner-Type Peanut Cultivars1
Authors: Branch W. D. , Baldwin J. A. , Culbreath A. K. 
Published: June 2010

Abstract
Tomato spotted wilt virus (TSWV) resistant, runner-type peanut (Arachis hypogaea L.) cultivars are the most important defense to control spotted wilt disease in southeast U.S. peanut production. The objective of this 3-yr (1999–01) study was to evaluate six TSWV-resistant, runner-type cultivars (Southern Runner, Florida MDR 98, C-99R, ViruGard, Georgia Green, and Georgia-OIR) at three different seeding rates (3, 5, and 7 seed/30.5 cm) in single conventional row patterns for possible genotype (GE) × seeding rate (SR) interaction at the Univ. of Georgia, Coastal Plain Experiment Station. The combined split-plot analyses of variance resulted in highly significant (P ≤ 0.01) GE × SR interaction, which indicates that not all six runner-type cultivars performed the same at each of these three seeding rates. A good example was the TSWV-resistant, runner-type peanut cultivar Georgia Green. It performed subpar at the below normal or lowest seeding rate; whereas at the highest seeding rate, Georgia Green and Georgia-OIR produced the highest pod yields and dollar value returns per hectare among all of these runner-type cultivars. TSWV disease incidence was also significantly lower for the TSWV-resistant Georgia Green cultivar at each of the two higher seeding rates compared to the lowest seeding rate.
Greenhouse Testing of Transgenic Peanut for Resistance to Sclerotinia minor
Authors: Chenault K. D. , Payton M. E. , Melouk H. A. 
Published: June 2010

Abstract
Fungal diseases of peanut, such as Sclerotinia blight caused by Sclerotinia minor Jagger, are responsible for increased production costs and yield losses of up to 50% for peanut producers in the Southwest, North Carolina, and Virginia. A few cultivars with moderate disease resistance, such as Southwest Runner, have been developed through traditional breeding practices. An urgent need exists for developing peanut cultivars that are resistant to the broad spectrum of fungal pathogens that pose a recurring threat to peanut health. Transgenic peanut plant lines containing anti-fungal genes have been produced from somatic embryos of the susceptible cultivar Okrun and tested under greenhouse conditions for resistance to S. minor by inoculation with a mycelial plug. Disease symptoms of lesion length and vascular collapse were recorded for 30 transgenic peanut lines, non-transgenic Okrun, and Southwest Runner. The reaction of the majority of transgenic peanut lines to S. minor infection was indistinguishable from that of the susceptible cultivar Okrun. However, three transgenic lines had a significant increase in resistance to S. minor as compared to Okrun, and one line demonstrated levels of resistance comparable to the moderately resistant cultivar Southwest Runner.
Composition and Decomposition of Peanut Residues in Georgia
Authors: Balkcom K. S. , Wood C. W. , Adams J. F. , Wood B. H. 
Published: June 2010

Abstract
Legumes typically mineralize rapidly and can contribute to nitrogen (N) requirements of succeeding crops, but limited information exists on the mineralizable N content of peanut (Arachis hypogaea L.) residue. The objective of this study was to determine net N mineralization from two types of peanut residue for two soil types. Aboveground peanut residue (cv. Georgia Green) was collected 1 d prior to digging (PRE) and immediately after peanut threshing (POST). Leaf and stem residues were mixed and analyzed for carbon (C), N, lignin, and cellulose. Peanut residue equivalent to 4.5 Mg/ha was applied to a Greenville fine sandy loam (fine, kaolinitic, thermic Rhodic Kandiudults) and a Tifton loamy sand (fine-loamy, kaolinitic, thermic Plinthic Kandiudult) and aerobically incubated for 98 d in the dark at 25 C to determine C and N mineralization. Each soil was incubated simultaneously, with and without residue. PRE harvest residue had lower C, lignin, and cellulose concen-trations, but higher N concentrations than POST harvest residue. Differences in residue quality corresponded to differences in cumulative C mineralized and C turnover for the Tifton soil, but did not result in differences for cumulative N mineralized or relative N mineralized within either soil type. These data indicate that peanut residue will not supply significant amounts of N to a subsequent crop for these two soil types.
Interaction of Flumioxazin with Dimethenamid or Metolachlor in Peanut (Arachis hypogaea L.)1
Authors: Grichar W. J. , Besler B. A. , Dotray P. A. , Johnson W. C. , Prostko E. R. 
Published: June 2010

Abstract
Field studies were conducted in various peanut growing regions of Texas and Georgia to study peanut response to flumioxazin alone or in combination with dimethenamid or metolachlor. In south Texas during 1997, flumioxazin plus metolachlor resulted in greater than 45% peanut stunt, while flumioxazin plus dimethenamid caused ≤20% peanut stunt. This stunting was attributed to cool, wet growing conditions. Peanut yields in flumioxazin plus metolachlor-treated plots were reduced 58% from peanut in the non-treated check. In 1999 and 2000, flumioxazin plus dimethenamid or metolachlor resulted in 15% or greater peanut stunt, but stunting was not constant across weather conditions or locations. In west Texas and Georgia no peanut stunting with flumioxazin was noted. At the Levelland, TX location, flumioxazin at 0.07 kg/ha plus metolachlor at 1.1 kg/ha reduced peanut yield 32% when compared with the non-treated check; while at the Pearsall, TX location, metolachlor or dimethenamid at 1.1 kg/ha, flumioxazin at 0.07 kg/ha plus dimethenamid at 1.5 kg/ha, and flumioxazin at 0.1 kg/ha plus dimethenamid at 1.1 kg/ha reduced peanut yield 24 to 48% when compared with the non-treated check. No yield reduction was noted in Georgia.
Control of Weeds in Peanut (Arachis hypogaea) Using Flumioxazin1
Authors: Grichar W. J. , Besler B. A. , Brewer K. D. 
Published: June 2010

Abstract
Flumioxazin in combination with ethalfluralin provided early-season control of eclipta, pitted morningglory, and Texas panicum. Metolachlor plus flumioxazin failed to adequately control pitted morningglory (less than 80%), while flumioxazin did not control yellow nutsedge. Eclipta control with flumioxazin plus dimethenamid, imazethapyr, or metolachlor combinations were at least 99%. When flumioxazin was used in combination with dimethenamid, imazethapyr, or metolachlor, late-season yellow nutsedge control was greater than 70%. Late-season eclipta control was greater than 80% with flumioxazin alone, dimethenamid plus flumioxazin applied preemergence (PRE), ethalfluralin applied preplant incorporated (PPI) followed by (fb) flumioxazin at 0.07 kg/ha applied PRE, imazethapyr plus flumioxazin at 0.09 kg/ha applied PRE, metolachlor plus flumioxazin combinations applied PRE, or ethalfluralin applied PPI fb imazapic applied postemergence (POST). Late-season pitted morningglory control was less than 65% for all herbicide programs. Flumioxazin alone controlled no greater than 25% pitted morningglory, while ethalfluralin plus flumioxazin combinations controlled less than 48%. Late-season, flumioxazin alone controlled less than 70% Texas panicum, while ethalfluralin alone provided 84% control. All ethalfluralin plus flumioxazin combinations controlled 70 to 83% Texas panicum, while imazethapyr or metolachlor plus flumioxazin combinations controlled less than 70%. Yellow nutsedge control was greater than 70% with imazethapyr or metolachlor plus flumioxazin combinations.
Evaluation of the Peanut Southern Corn Rootworm Advisory
Authors: Herbert D. A. , Malone S. , Brandenburg R. L. , Royals B. M. 
Published: June 2010

Abstract
The southern corn rootworm, Diabrotica undecimpunctata howardi Barber, is an annual soil insect pest of peanut (Arachis hypogaea L.) in Virginia, North Carolina, Texas, and other peanut growing states. Larvae feed on developing pods causing direct yield loss and indirect damage by allowing entry of secondary pathogens. Because southern corn rootworm is a soil pest, scouting is difficult and producers make preventive treatments without knowledge of actual pest abundance. A predictive index for southern corn rootworm injury was evaluated using 392 field case studies conducted in Virginia and North Carolina from 1997 to 2001. Factors influencing the index score (soil texture, soil drainage class, planting date, cultivar resistance, and field history of rootworm damage), and point assignments for predicting low, moderate, and high-risk fields were analyzed. The goal of this project was to determine which combination of factors provided the highest percentage of correct risk predictions. The best index combination used all five factors to determine the total point score, with 70 or more points indicating a high-risk field, 55 to 65 points a moderate-risk field, and less than or equal to 50 points a low-risk field. Growers who use the index eliminate a preventive insecticide treatment in low-risk and some moderate-risk fields. The index correctly predicted the level of pod damage in 45% (177 of 392) of the field case studies. Insecticide was correctly recommended in 46 fields. Thirty-three percent (131 of 392) of the fields were correctly identified as not needing treatment. There were 209 cases where there was an overestimation of pod damage with predictions of either a high or moderate level when only a low level occurred. In these cases, an insecticide treatment would have been recommended and an average of 6.1 and 2.6% pod damage, respectively, would have been prevented. Conversely, there were very few fields that should have been treated but were not treated (6 of 392). Overall, use of the index would have protected fields from pod damage and potential loss 98.5% of the time.
An Allotetraploid Model for the Inheritance of Wine Testa Color in Peanut1
Authors: Pittman R. N. , Kirby J. S. , Banks D. J. 
Published: June 2010

Abstract
Wine-colored peanut seeds originate as mutants and appear later as segregates in farmer's fields. Off-color seeds are unwanted by shellers and manufacturers. The inheritance of wine testa is complex and among the least understood of the basic testa colors. The objective of this study was to determine the inheritance of wine testa color. True breeding genotypes for tan and wine testa color were crossed and progenies analyzed for testa color. A new genetic model is presented to explain the inheritance of the wine-colored seed trait. A tetragenic model, based on a 207:49 (tan to wine) F2 segregation pattern, best described the data and assumes two sets of paired genes representing two genomes. At least one pair of homozygous recessive alleles in each set is necessary for expression of the wine-colored trait. Using parents in peanut breeding programs that are homozygous dominant at all four loci will help reduce the rate at which wine-colored seeds appear in farmer's fields.
Variability Among Aflatoxin Test Results on Runner Peanuts Harvested From Small Field Plots1
Authors: Whitaker T. B. , Dorner J. W. , Giesbrecht F. G. , Slate A. B. 
Published: June 2010

Abstract
An experiment was conducted to determine the variability associated with aflatoxin contamination of peanuts from plants grown in specified row lengths. Runner peanuts (cv. Georgia Green) were planted in 10, 76.2 m rows (20 seed/m) and grown using standard production practices. Plants were exposed to natural late-season drought conditions making the peanuts susceptible to preharvest aflatoxin contamination. Plants were mechanically dug, inverted, and separated into 500 plots of 1.5 m single rows. Peanuts from each numerically identified plot were harvested with a mechanical picker, dried to 8% kernel moisture (wet basis), shelled, and analyzed for aflatoxin by high performance liquid chromatography (HPLC). The average kernel mass and weighted average aflatoxin concentration for all plots was 131 g and 2278 ng/g, respectively. The kernel mass varied among the 500 plots from a low of 4 g to a maximum of 283 g. The aflatoxin concentration among the 500 plots varied from a low of 0 ng/g to a maximum of 32,142 ng/g. The standard deviation among the 500 plot aflatoxin values was 4061. The standard deviation among sample concentrations for this field study was very similar to previous studies that measured the standard deviation among sample concentrations taken from bulk farmers' stock lots. Increasing plot length decreased the standard deviation among plot aflatoxin values as predicted by statistical theory. For example, increasing plot row length by a factor of four, or from 1.5 to 6 m, decreased the standard deviation by a factor of two, or from 4061 to 2031. A regression equation was developed to predict the effect of plot row length on the variability among aflatoxin plot values. This information is useful for designing field plot experiments to test various strategies for reducing or preventing preharvest aflatoxin contamination.
Combined Effects of Biological Control Formulations, Cultivars, and Fungicides on Preharvest Colonization and Aflatoxin Contamination of Peanuts by Aspergillus Species
Authors: Dorner J. W. 
Published: June 2010

Abstract
A 3-yr field study was conducted to determine the effect of biological control formulations of nontoxigenic strains of Aspergillus flavus and A. parasiticus, peanut cultivars, and fungicides on preharvest aflatoxin contamination of peanuts. Formulation treatments consisted of (a) no biocontrol treatment, (b) the fungi cultured on rice via solid-state fermentation, (c) conidia of the fungi coated onto the surface of rice, and (d) conidia coated onto the surface of wheat (year one) or hulled barley (years two and three). Experiments consisted of factorial combinations of the four formulation treatments, two peanut cultivars (Florunner or Georgia Green), and two fungicide treatments (chlorothalonil or combinations of chlorothalonil and tebuconazole). Florunner and Georgia Green peanuts were each planted in 32 individual plots consisting of six rows 15.2 m in length. Biological control formulations, consisting of a mixture of nontoxigenic strains of A. flavus (NRRL 21882) and A. parasiticus (NRRL 21369), were applied to the same plots in each of the 3 yr at a rate of 56 kg/ha. Foliar applications of fungicides were made as recommended for control of leaf spot, with one treatment being full-season applications of chlorothalonil, and the other being two applications of chlorothalonil followed by four applications of tebuconazole and remaining applications of chlorothalonil. Only in year two of the study was late-season drought sufficient to produce preharvest aflatoxin contamination. All biocontrol formulation treatments produced significant reductions in aflatoxin compared with untreated controls, averaging 81%. There was also a significant cultivar effect on aflatoxin with Georgia Green averaging 119 μg/kg compared with 402 μg/kg for Florunner. No differences were observed between the two fungicide treatments, and there was no interaction among the three factors. Analysis of soil for populations of A. flavus and A. parasiticus throughout the study showed that all formulations, except the conidia-coated wheat in the first year, were effective in delivering competitive levels of the nontoxigenic strains. In the third year, which did not result in significant aflatoxin contamination, analysis of peanuts for fungal colonization showed no significant differences among biocontrol treatments (including control) for total amounts of A. flavus and A. parasiticus in peanuts. However, the incidence of toxigenic isolates in peanuts was significantly reduced by all three biocontrol formulations.
Peanut Weed Control With and Without Acetolactate Synthase-inhibiting Herbicides1
Authors: Wehtje G. , Brecke B. 
Published: June 2010

Abstract
Field studies were conducted in Florida and Alabama during 2001 and 2002 to compare weed control systems for peanut (Arachis hypogaea L.) that included only the herbicides registered on peanut that do not inhibit aceto hydroxyl acid synthase (AHAS). Three non-AHAS systems were identified that consistently preformed equivalent to imazapic, i.e., an AHAS-inhibiting herbicide that is very effective in peanut. These systems were either S-metolachlor plus flumioxazin, S-metolachlor plus S-dimethenamid, or S-metolachor plus norflurazon applied preemergence (PRE), followed by paraquat plus bentazon plus 2,4-DB applied postemergence. Greenhouse studies established that tank mixtures of S-metolachlor plus flumioxazin and S-metolachor plus norflurazon applied PRE were synergistic with respect to yellow nutsedge (Cyperus esculentus L.) control. This synergism may contribute to the excellent performance of these S-metolachlor-containing tank mixtures in the field. Identification of systems which utilize herbicides with modes of action other than AHAS inhibition could offer rotational alternatives to delay the emergence of AHAS-resistant weed biotypes, or alternatives should such biotypes become problematic.