bluuebunny/crossref_metadata_2025_split huggingface dataset:
Predicting the public’s seeking intention of COVID-19 risk information through the comparison of PRISM and expanded PRISM
Authors: Liu Mei , Xiong Danli , He Mengru 
Published: May 2024

AbstractThe applicability of the main theories of risk information seeking to novel risk scenarios is not well understood. According to Hovick, Kahlor, and Liang (Journal of Health Communication 19:511-527, 2014), this study retested the Planned Risk Information Seeking Model (PRISM) and examined the impact of additional variables on the model in order to investigate the critical factors (expanded PRISM) that affect people’s intention to seek information about COVID-19 risks and determine whether the PRISM model is more appropriate in this context than expanded PRISM based on survey data from 1031 Chinese adults. The results showed that PRISM fit the data better. Furthermore, future study should focus on two new variables: source beliefs and past information seeking. Theoretical and practical implications are considered.
Import of digital products and production fragmentation
Authors: Liu Jiaqi , Sun Puyang , Yu Chunhai 
Published: May 2024

AbstractEmpowering traditional manufacturing through the digital economy can not only enhance the competitiveness of Chinese manufacturing enterprises, but also enhance the degree of specialization of enterprises by optimizing the industrial structure, thus enhancing the embeddedness of Chinese enterprises in the global value chain. Based on this background, on the one hand, this paper extracts and integrates digital product information from China Customs data. On the other hand, this paper constructs the number of production processes of enterprises as a proxy variable for production fragmentation. Finally, by matching these two databases, we get the production segmentation data of Chinese enterprises' digital product import from 2000 to 2014. We found that improving the penetration degree of digital technology by importing digital products can effectively promote the production fragmentation. In order to further clarify the mechanism, we divided the digital products into final and intermediate kinds and found that firms importing digital final goods have a larger marginal impact on their production fragmentation.
Caring for Valid Sexual Consent
Authors: Israel Eli Benjamin 
Published: May 2024

Abstract
When philosophers consider factors compromising autonomy in consent, they often focus solely on the consent-giver's agential capacities, overlooking the impact of the consent-receiver's conduct on the consensual character of the activity. In this paper, I argue that valid consent requires justified trust in the consent-receiver to act only within the scope of consent. I call this the Trust Condition (TC), drawing on Katherine Hawley's commitment account of trust. TC constitutes a belief that the consent-receiver is capable and willing to act as we expect from them. If such trust is not warranted, I argue, consent lacks the appropriate normative grounds. After establishing TC, I explore its application in the sexual arena, asserting that, due to the non-contractual dimensions of sexual activity—such as the dynamic nature of sexual desires and the absence of external factors effectively binding sexual partners to the terms of consent—trust is warranted in sexual consent by means of care. I define care as a special sensitivity and attention toward the partner's will and discuss how this approach leads to safer intimate relationships in practice.
Mechanically induced localisation of SECONDARY WALL INTERACTING bZIP is associated with thigmomorphogenic and secondary cell wall gene expression
Authors: Coomey Joshua H. , MacKinnon Kirk J.-M. , McCahill Ian W. , Khahani Bahman , Handakumbura Pubudu P. , Trabucco Gina M. , Mazzola Jessica , Leblanc Nicole A. , Kheam Rithany , Hernandez-Romero Miriam , Barry Kerrie , Liu Lifeng , Lee Ji E. , Vogel John P. , O’Malley Ronan C. , Chambers James J. , Hazen Samuel P. 
Published: May 2024

Abstract
Plant growth requires the integration of internal and external cues, perceived and transduced into a developmental programme of cell division, elongation and wall thickening. Mechanical forces contribute to this regulation, and thigmomorphogenesis typically includes reducing stem height, increasing stem diameter, and a canonical transcriptomic response. We present data on a bZIP transcription factor involved in this process in grasses. Brachypodium distachyon SECONDARY WALL INTERACTING bZIP (SWIZ) protein translocated into the nucleus following mechanostimulation. Classical touch-responsive genes were upregulated in B. distachyon roots following touch, including significant induction of the glycoside hydrolase 17 family, which may be unique to grass thigmomorphogenesis. SWIZ protein binding to an E-box variant in exons and introns was associated with immediate activation followed by repression of gene expression. SWIZ overexpression resulted in plants with reduced stem and root elongation. These data further define plant touch-responsive transcriptomics and physiology, offering insights into grass mechanotranduction dynamics.
Doing being an average teenager: Deploying ordinariness as subversive disability performance in presentational media
Authors: Li Xiaowei May 
Published: May 2024

Abstract
Scholars have shown how speakers are inclined to discursively position themselves as ‘ordinary’ in order to claim and benefit from membership in a socially unmarked category, and that the effect of ‘being ordinary’ is an effortful communicative achievement (e.g. Sacks 1984). This study re-examines and extends such insight by focusing on socially marked individuals—people with disabilities—and considers the effect of inhabiting a nonnormative body has on the semiotic production of self as ordinary. The multimodal self-presentation of Nikki Lilly, a popular disabled YouTuber, showcases the tension between inhabiting a physically anomalous body and projecting ‘an average teenager’ persona. The analysis of the vlogger's YouTube and Instagram posts shows that resignifying the nonnormative body and self as symbolically unmarked hinges on recruiting hypernormative gendered resources. I argue that by exaggerating normality, Nikki Lilly's recognized ‘ordinary’ self-presentation enunciates normalcy as an illusory imperative and materializes as subversive the performance of disability. (Nikki Lilly, embodiment, multimodality, presentational media, disability, ordinariness, normativity)*
Prevalence of nontuberculous mycobacteria and the emergence of rare species in Henan Province, China
Authors: Hongmei Shi , Ruixia Liang , Jiankang Li , Han Wu , Chang Zhao , Yan Mo Yan , Kang Wang Zi , Li Li Fu , Zhi Ding Cheng , Xin Liu 
Published: May 2024

Abstract
Nontuberculous mycobacteria (NTM) is a large group of mycobacteria other than the Mycobacterium tuberculosis complex and Mycobacterium leprae. Epidemiological investigations have found that the incidence of NTM infections is increasing in China, and it is naturally resistant to many antibiotics. Therefore, studies of NTM species in clinical isolates are useful for understanding the epidemiology of NTM infections. The present study aimed to investigate the incidence of NTM infections and types of NTM species. Of the 420 samples collected, 285 were positive for M. tuberculosis, 62 samples were negative, and the remaining 73 samples contained NTM, including 35 (8.3%) only NTM and 38 (9%) mixed (M. tuberculosis and NTM). The most prevalent NTM species were Mycobacterium intracellulare (30.1%), followed by Mycobacterium abscessus (15%) and M. triviale (12%). M. gordonae infection was detected in 9.5% of total NTM-positive cases. Moreover, this study reports the presence of Mycobacterium nonchromogenicum infection and a high prevalence of M. triviale for the first time in Henan. M. intracellulare is the most prevalent, accompanied by some emerging NTM species, including M. nonchromogenicum and a high prevalence of M. triviale in Henan Province. Monitoring NTM transmission and epidemiology could enhance mycobacteriosis management in future.
Analysis of Uncertainties and Associated Convective Processes in Simulations of Extreme Precipitation Over Cities With a Regional Earth System Model: A Case Study
Authors: Li Zhenghui , Luo Yali , Chen Feng 
Published: May 2024

AbstractThis study utilizes the Weather Research and Forecasting model coupled with an atmospheric chemistry model, a multi‐layer urban canopy model (UCM), and a building energy model to simulate the extreme rainfall event influencing the Guangzhou city in South China on 7 May 2017. By employing small variations in the longwave emissivity of buildings within the UCM, 11 convective‐permitting experiments are conducted. The maximal 18‐hr and hourly rainfall accumulation vary from a 2‐year return period to as high as a 20 or 40‐year return period with notable spatial differences. Comparisons between the more accurate group (GOOD) and less accurate group (POOR) simulations highlight that some minor differences in the near‐surface air thermodynamic conditions in urban area could lead to substantial differences in local convection and its impacts on subsequent convective systems. With persistent transportation of warm, moist airflows from the northern South China Sea, formation of a slow‐moving mesoscale outflow boundary to the north of the urban agglomeration leads to the development of a quasi‐stationary, compactly structured meso‐γ‐scale rainstorm in the GOOD simulations. The stronger low‐level to near‐surface convergence and mid‐level cyclonic shear within this system substantially enhance low‐level updrafts, leading to increased microphysical production and stronger horizontal advection of rainwater within the system. These findings offer some process‐based understanding about the uncertainties in simulating urban extreme rainfall and underscore the need to develop ensemble forecasting methods for convection‐permitting numerical models that incorporate increasingly complicated representations of anthropogenic influences.
Synthesis of Elliptically Shaped Aperiodic Antenna Arrays Subject to Complex Design Constraints
Authors: Geerarts J. M. E. , Theis G. , Smolders A. B. , Caratelli D. 
Published: May 2024

AbstractState‐of‐the‐art conformal array pattern synthesis techniques typically suffer from a significant computational burden. This article builds upon the auxiliary array pattern synthesis technique, allowing the technique to work on elliptical contours. This deterministic method is both computationally efficient and capable of handling complex design cases with additional design constraints. In order to highlight the versatility and applicability, the method has been applied to an elliptical arc array comprised of Rampart antennas and an elliptical ring array using dipole elements. The results have been validated through full‐wave electromagnetic simulations, as well as physical measurements. Both arrays presented show good agreement with the proposed synthesis technique. Highlighting the ability to satisfy additional design constraints, such as enforcing a minimal element spacing or having a controlled beamformer‐network complexity.
A Novel Local‐Inertial Formulation Representing Subgrid Scale Topographic Effects for Urban Flood Simulation
Authors: Nithila Devi N. , Kuiry Soumendra Nath 
Published: May 2024

AbstractThe local‐inertial approximations of the shallow water equations (SWEs) have been used for flood forecasting at larger spatial scales owing to the improved computational efficiency and similar accuracy compared to the full 2D SWEs. With the availability of high‐resolution elevation data, the complex terrain of urban areas with various small‐scale features is represented well. Even for a local‐inertial model, utilizing such high‐resolution elevation data in flood simulations of urbanized areas increases the computational cost. A subgrid‐based local‐inertial formulation that permits large numerical grid size for computations while preserving the within‐grid topography is proposed to circumvent this. The subgrid topography can be incorporated into the coarse numerical grid computations by estimating the hydraulic properties, namely, volume and face area, based on water surface elevation variations of the associated high‐resolution terrain. The pre‐stored hydraulic properties are then used to dynamically update the hydraulic variables during the execution of the local‐inertial model. Idealized and real‐world test cases were simulated to illustrate the advantages of the proposed model. The proposed subgrid model performs better in capturing flood depth around subgrid‐scale features such as streets, highways, minor canals, etc., than the simple grid‐averaged local‐inertial models of the same grid size. The proposed model is faster than the existing local‐inertial model (e.g., LISFLOOD‐FP) (∼21–34 times) and the full 2D model (e.g., HEC‐RAS 2D) (∼361–660 times) of similar accuracy in the slow‐rising flood applications. Thus, the subgrid local‐inertial model holds promise in real‐time flood inundation forecasting, resolving smaller urban features.
Haploidentical hematopoietic cell transplantation with or without an unrelated cord blood unit for adult acute myeloid leukemia: a multicenter, randomized, open-label, phase 3 trial
Authors: Zhou Biqi , Chen Jia , Liu Tianhui , Ye Yishan , Zhang Yanming , Ding Yiyang , Liu Hong , Zhu MingQing , Ma Xiao , Li Xiaoli , Zhao Longfei , Lin Zhihong , Huang He , Xu Yang , Wu Depei 
Published: May 2024

AbstractsCoinfusion of unrelated cord blood (UCB) units in haploidentical hematopoietic cell transplantation (haplo-HCT) (haplo-cord HCT) for hematopoietic malignancies showed promising results in previous reports, but the efficiency of haplo-cord HCT in acute myeloid leukemia (AML) still lacks sufficient evidence. This multicenter, randomized, phase 3 trial (ClinicalTrials.gov NCT03719534) aimed to assess the efficacy and safety of haplo-cord HCT in AML patients. A total of 268 eligible patients aged 18–60 years, diagnosed with measurable residual disease in AML (excluding acute promyelocytic leukemia), with available haploidentical donors and suitable for allotransplantation, were randomly allocated (1:1) to receive haplo-cord HCT (n = 134) or haplo-HCT (n = 134). The 3-year overall survival (OS) was the primary endpoint in this study. Overall median follow-up was 36.50 months (IQR 24.75–46.50). The 3-year OS of Haplo-cord HCT group was better than haplo-HCT group (80.5%, 95% confidence interval [CI]: 73.7–87.9 vs. 67.8% 95% CI 60.0–76.5, p = 0.013). Favorable progression-free survival (70.3%, 95% CI 62.6–78.8 vs. 57.6%, 95% CI 49.6–67.0, p = 0.012) and cumulative incidence of relapse (12.1%, 95% CI 12.0–12.2 vs. 30.3%, 95% CI 30.1–30.4, p = 0.024) were observed in haplo-cord HCT group. Grade 3–4 adverse events (AEs) within two years posttransplantation in the two groups were similar. Haplo-cord HCT patients exhibited a faster cumulative incidence of neutrophil recovery (p = 0.026) and increased T-cell reconstitution in the early period posttransplantation. Haplo-cord HCT can improve OS in AML patients without excessive AEs, which may exert additional benefits for recipients of haplo-HCT.
Combination therapy with oncolytic virus and T cells or mRNA vaccine amplifies antitumor effects
Authors: Fu Rao , Qi Ruoyao , Xiong Hualong , Lei Xing , Jiang Yao , He Jinhang , Chen Feng , Zhang Liang , Qiu Dekui , Chen Yiyi , Nie Meifeng , Guo Xueran , Zhu Yuhe , Zhang Jinlei , Yue Mingxi , Cao Jiali , Wang Guosong , Que Yuqiong , Fang Mujing , Wang Yingbin , Chen Yixin , Cheng Tong , Ge Shengxiang , Zhang Jun , Yuan Quan , Zhang Tianying , Xia Ningshao 
Published: May 2024

AbstractAntitumor therapies based on adoptively transferred T cells or oncolytic viruses have made significant progress in recent years, but the limited efficiency of their infiltration into solid tumors makes it difficult to achieve desired antitumor effects when used alone. In this study, an oncolytic virus (rVSV-LCMVG) that is not prone to induce virus-neutralizing antibodies was designed and combined with adoptively transferred T cells. By transforming the immunosuppressive tumor microenvironment into an immunosensitive one, in B16 tumor-bearing mice, combination therapy showed superior antitumor effects than monotherapy. This occurred whether the OV was administered intratumorally or intravenously. Combination therapy significantly increased cytokine and chemokine levels within tumors and recruited CD8+ T cells to the TME to trigger antitumor immune responses. Pretreatment with adoptively transferred T cells and subsequent oncolytic virotherapy sensitizes refractory tumors by boosting T-cell recruitment, down-regulating the expression of PD-1, and restoring effector T-cell function. To offer a combination therapy with greater translational value, mRNA vaccines were introduced to induce tumor-specific T cells instead of adoptively transferred T cells. The combination of OVs and mRNA vaccine also displays a significant reduction in tumor burden and prolonged survival. This study proposed a rational combination therapy of OVs with adoptive T-cell transfer or mRNA vaccines encoding tumor-associated antigens, in terms of synergistic efficacy and mechanism.
De novo generation of multi-target compounds using deep generative chemistry
Authors: Munson Brenton P. , Chen Michael , Bogosian Audrey , Kreisberg Jason F. , Licon Katherine , Abagyan Ruben , Kuenzi Brent M. , Ideker Trey 
Published: May 2024

AbstractPolypharmacology drugs—compounds that inhibit multiple proteins—have many applications but are difficult to design. To address this challenge we have developed POLYGON, an approach to polypharmacology based on generative reinforcement learning. POLYGON embeds chemical space and iteratively samples it to generate new molecular structures; these are rewarded by the predicted ability to inhibit each of two protein targets and by drug-likeness and ease-of-synthesis. In binding data for >100,000 compounds, POLYGON correctly recognizes polypharmacology interactions with 82.5% accuracy. We subsequently generatede-novocompounds targeting ten pairs of proteins with documented co-dependency. Docking analysis indicates that top structures bind their two targets with low free energies and similar 3D orientations to canonical single-protein inhibitors. We synthesize 32 compounds targeting MEK1 and mTOR, with most yielding >50% reduction in each protein activity and in cell viability when dosed at 1–10 μM. These results support the potential of generative modeling for polypharmacology.
Primordial germ cell DNA demethylation and development require DNA translesion synthesis
Authors: Shah Pranay , Hill Ross , Dion Camille , Clark Stephen J. , Abakir Abdulkadir , Willems Jeroen , Arends Mark J. , Garaycoechea Juan I. , Leitch Harry G. , Reik Wolf , Crossan Gerry P. 
Published: May 2024

AbstractMutations in DNA damage response (DDR) factors are associated with human infertility, which affects up to 15% of the population. The DDR is required during germ cell development and meiosis. One pathway implicated in human fertility is DNA translesion synthesis (TLS), which allows replication impediments to be bypassed. We find that TLS is essential for pre-meiotic germ cell development in the embryo. Loss of the central TLS component, REV1, significantly inhibits the induction of human PGC-like cells (hPGCLCs). This is recapitulated in mice, where deficiencies in TLS initiation (Rev1-/- or PcnaK164R/K164R) or extension (Rev7 -/-) result in a > 150-fold reduction in the number of primordial germ cells (PGCs) and complete sterility. In contrast, the absence of TLS does not impact the growth, function, or homeostasis of somatic tissues. Surprisingly, we find a complete failure in both activation of the germ cell transcriptional program and in DNA demethylation, a critical step in germline epigenetic reprogramming. Our findings show that for normal fertility, DNA repair is required not only for meiotic recombination but for progression through the earliest stages of germ cell development in mammals.
AnnoSpat annotates cell types and quantifies cellular arrangements from spatial proteomics
Authors: Mongia Aanchal , Zohora Fatema Tuz , Burget Noah G. , Zhou Yeqiao , Saunders Diane C. , Wang Yue J. , Brissova Marcela , Powers Alvin C. , Kaestner Klaus H. , Vahedi Golnaz , Naji Ali , Schwartz Gregory W. , Faryabi Robert B. 
Published: May 2024

AbstractCellular composition and anatomical organization influence normal and aberrant organ functions. Emerging spatial single-cell proteomic assays such as Image Mass Cytometry (IMC) and Co-Detection by Indexing (CODEX) have facilitated the study of cellular composition and organization by enabling high-throughput measurement of cells and their localization directly in intact tissues. However, annotation of cell types and quantification of their relative localization in tissues remain challenging. To address these unmet needs for atlas-scale datasets like Human Pancreas Analysis Program (HPAP), we develop AnnoSpat (Annotator and Spatial Pattern Finder) that uses neural network and point process algorithms to automatically identify cell types and quantify cell-cell proximity relationships. Our study of data from IMC and CODEX shows the higher performance of AnnoSpat in rapid and accurate annotation of cell types compared to alternative approaches. Moreover, the application of AnnoSpat to type 1 diabetic, non-diabetic autoantibody-positive, and non-diabetic organ donor cohorts recapitulates known islet pathobiology and shows differential dynamics of pancreatic polypeptide (PP) cell abundance and CD8+ T cells infiltration in islets during type 1 diabetes progression.
Focal adhesion kinase-YAP signaling axis drives drug-tolerant persister cells and residual disease in lung cancer
Authors: Haderk Franziska , Chou Yu-Ting , Cech Lauren , Fernández-Méndez Celia , Yu Johnny , Olivas Victor , Meraz Ismail M. , Barbosa Rabago Dora , Kerr D. Lucas , Gomez Carlos , Allegakoen David V. , Guan Juan , Shah Khyati N. , Herrington Kari A. , Gbenedio Oghenekevwe M. , Nanjo Shigeki , Majidi Mourad , Tamaki Whitney , Pourmoghadam Yashar K. , Rotow Julia K. , McCoach Caroline E. , Riess Jonathan W. , Gutkind J. Silvio , Tang Tracy T. , Post Leonard , Huang Bo , Santisteban Pilar , Goodarzi Hani , Bandyopadhyay Sourav , Kuo Calvin J. , Roose Jeroen P. , Wu Wei , Blakely Collin M. , Roth Jack A. , Bivona Trever G. 
Published: May 2024

AbstractTargeted therapy is effective in many tumor types including lung cancer, the leading cause of cancer mortality. Paradigm defining examples are targeted therapies directed against non-small cell lung cancer (NSCLC) subtypes with oncogenic alterations in EGFR, ALK and KRAS. The success of targeted therapy is limited by drug-tolerant persister cells (DTPs) which withstand and adapt to treatment and comprise the residual disease state that is typical during treatment with clinical targeted therapies. Here, we integrate studies in patient-derived and immunocompetent lung cancer models and clinical specimens obtained from patients on targeted therapy to uncover a focal adhesion kinase (FAK)-YAP signaling axis that promotes residual disease during oncogenic EGFR-, ALK-, and KRAS-targeted therapies. FAK-YAP signaling inhibition combined with the primary targeted therapy suppressed residual drug-tolerant cells and enhanced tumor responses. This study unveils a FAK-YAP signaling module that promotes residual disease in lung cancer and mechanism-based therapeutic strategies to improve tumor response.