bluuebunny/crossref_metadata_2025_split huggingface dataset:
SMYD2 induced PGC1α methylation promotes stemness maintenance of glioblastoma stem cells
Authors: Li Mengdie , Zhang Zhixiang , He Liuguijie , Wang Xiefeng , Yin Jianxing , Wang Xiuxing , You Yongping , Qian Xu , Ge Xin , Shi Zhumei 
Published: May 2024

Abstract

Background
The high fatality rate of glioblastoma (GBM) is attributed to glioblastoma stem cells (GSCs), which exhibit heterogeneity and therapeutic resistance. Metabolic plasticity of mitochondria is the hallmark of GSCs. Targeting mitochondrial biogenesis of GSCs is crucial for improving clinical prognosis in GBM patients.

Methods
SMYD2-induced PGC1α methylation and followed nuclear export are confirmed by co-immunoprecipitation, cellular fractionation, and immunofluorescence. The effects of SMYD2/PGC1α/CRM1 axis on GSCs mitochondrial biogenesis are validated by oxygen consumption rate, ECAR, and intracranial glioma model.

Results
PGC1α methylation causes the disabled mitochondrial function to maintain the stemness, thereby enhancing the radio-resistance of GSCs. SMYD2 drives PGC1α K224 methylation (K224me), which is essential for promoting the stem-like characteristics of GSCs. PGC1α K224me is preferred binding with CRM1, accelerating PGC1α nuclear export and subsequent dysfunction. Targeting PGC1α methylation exhibits significant radiotherapeutic efficacy and prolongs patient survival.

Conclusions
These findings unveil a novel regulatory pathway involving mitochondria that govern stemness in GSCs, thereby emphasizing promising therapeutic strategies targeting PGC1α and mitochondria for the treatment of GBM.
The Brazilian international patient summary initiative
Authors: de Faria Leao Beatriz , Costa Italo Macedo do Amaral , Machado Joice , de Assis Molla Monalisa , Zamarro Aline Rodrigues , Motter Fabiane Raquel , Oliveira Gabriel Gausmann , Costa Karla L de A Calvette , de Mello Blanda Helena , Souza Elivan Silva , Neves Gabriella Nunes , de Melo Matos Robson Willian , dos Santos Paula Xavier , de Oliveira José Eduardo Bueno , Gadenz Sabrina Dalbosco 
Published: May 2024

ABSTRACT
This paper describes the development of the Brazilian version of the International Patient Summary (IPS). A joint technical team is developing the project from Syrian-Lebanese Hospital, São Paulo, and the Coordination of Innovation and Informatics in Health, Secretariat of Information and Digital Health, Brazilian Ministry of Health. The five-phased approach used in the development of Brazil IPS starts with the identification of the Brazilian Ministry of Health national terminologies used to represent concepts for problems, diagnosis and procedures, their upload in a terminology server and mapping to IPS ValueSets, the Fast Healthcare Interoperability Resources profiles customization and, finally, the complete Brazil IPS Implementation Guide is described.
2023 Record marine heat waves: coral reef bleaching HotSpot maps reveal global sea surface temperature extremes, coral mortality, and ocean circulation changes
Authors: Goreau Thomas J F , Hayes Raymond L 
Published: May 2024

Abstract
Coral reefs, the most sensitive ecosystem to high temperature, are on the precipice of mass extinction from global warming [1, 2]. 2023 was the hottest year in recorded history on land and in the sea, with dramatic and unexpected temperature increases [3, 4]. Coral Reef Bleaching HotSpot maps provide unique insight into global ocean circulation changes in response to greenhouse gas (GHG) forcing that caused dramatic global temperature rises [1, 2]. The highest excess daily air temperatures recorded in 175 countries, as well as the most prolonged excessive sea surface temperatures, were centered around Jamaica. 2023 marked the worst coral reef bleaching yet in the Northern Hemisphere, with the Southern Hemisphere poised to follow in early 2024. The HotSpot maps strongly suggest accelerated ocean poleward heat transport, slowdown in upwelling, and decreased deep water formation linked to sharply increased 2023 anomalous sea surface and air temperatures. The 2023 distribution of severe heat and bleaching follows both spatial patterns and temporal trends first shown from a baseline 1982–2001 global SST trend analysis [5]. Increased warming of both hot and cold ocean currents shows that horizontal mixing of tropical heat to the poles is accelerating, and that vertical mixing with cold deep water is slowing down, leading to increased ocean stratification, which will cause sea temperature to increase more rapidly and CO2 mixing with the deep ocean to decrease.
An Integrated, Process Oriented Multilevel Theory of Team Adaptation
Authors: Misisco Anthony T. , Kozlowski Steve W. J. 
Published: May 2024

Abstract
Uncertainty is inherent to the team adaptation process. Stereotypically, team adaptation is operationalized as a response to a negative, external environmental occurrence. This chapter establishes the foundation for a more expansive and integrated multilevel theory of team adaptation to uncertainty. The authors suggest that uncertainty can be created and increased cognizantly by individual actors, can emerge endogenously, and can be positively valent. These principles undergird a process-oriented multilevel theory of team adaptation. First, the authors discuss the implications of teams engaging in deliberate, positive, internal uncertainty creation processes for the long-term development of team-level adaptive capabilities. Next, the authors propose a model of uncertainty that addresses the compilational process through which team members respond to adaptive performance episodes. Conceptualizing the team adaptation process occurring through compilational, patterned networks of interaction advances a model of adaptation that advances theory beyond simplistic compositional models that do not adequately capture the multilevel nature of adaptation.
Physics of nova outbursts: Theoretical models of classical nova outbursts with optically thick winds on 1.2 M⊙ and 1.3 M⊙ white dwarfs
Authors: Kato Mariko , Saio Hideyuki , Hachisu Izumi 
Published: May 2024

Abstract
We present time-dependent nova outburst models with optically thick winds for 1.2 and 1.35$\, M_{\odot }$ white dwarfs (WDs) with a mass-accretion rate of $5 \times 10^{-9}\, M_{\odot }$ yr−1 and for a 1.3$\, M_{\odot }$ WD with $2 \times 10^{-9}\, M_{\odot }$ yr−1. The X-ray flash occurs 11 d before the optical peak of the 1.2$\, M_{\odot }$ WD and 2.5 d before the peak of the 1.3$\, M_{\odot }$ WD. The wind mass-loss rate of the 1.2$\, M_{\odot }$ WD (1.3$\, M_{\odot }$ WD) reaches a peak of $6.4 \times 10^{-5}\, M_{\odot }$ yr−1 ($7.4 \times 10^{-5}\, M_{\odot }$ yr−1) at the epoch of the maximum photospheric expansion with the lowest photospheric temperature of log Tph (K) = 4.33 (4.35). The nuclear energy generated during the outburst is lost in the form of radiation (61% for the 1.2$\, M_{\odot }$ WD; 47% for the 1.3$\, M_{\odot }$ WD), gravitational energy of ejecta (39%; 52%), and kinetic energy of the wind (0.28%; 0.29%). We found an empirical relation for fast novae between the time to optical maximum from the outburst tpeak and the expansion timescale τexp. With this relation, we are able to predict the time to optical maximum tpeak from the ignition model (at t = 0) without following a time-consuming nova wind evolution.
Evaluation of thoracic sympathetic ganglion block as a predictor for response to ketamine infusion therapy and spinal cord stimulation in patients with chronic upper extremity pain
Authors: Kim Jeongsoo , Kim Hangaram , Kim Jae Eun , Yoo Yongjae , Moon Jee Youn 
Published: May 2024

Abstract

Objective
To investigate the predictive value of thoracic sympathetic ganglion block (TSGB) in response to ketamine infusion therapy (KIT) and spinal-cord stimulation (SCS) in patients with chronic upper-extremity pain including complex regional pain syndrome (CRPS).

Design
Retrospective

Setting
Tertiary hospital single-center

Subjects
Patients who underwent TSGB receiving KIT or SCS within a 3-year window.

Methods
Positive TSGB outcomes were defined as ≥2 0–10 Numerical Rating Scale (NRS) score reduction at 2 weeks post-procedure. Positive KIT and SCS outcomes were determined by ≥2 NRS score reduction at 2–4 weeks post-KIT and ≥4 NRS score reduction at 2–4 weeks post-SCS implantation, respectively.

Results
Among 207 patients who underwent TSGB, 38 received KIT and 34 underwent SCS implantation within 3 years post-TSGB; 33 patients receiving KIT and 32 patients receiving SCS were included. Among 33 patients who received KIT, 60.6% (n = 20) reported a ≥ 2 0–10 NRS pain-score reduction. Positive response to TSGB occurred in 70.0% (n = 14) KIT responders, significantly higher than that in 30.8% (n = 4) KIT non-responders. Multivariable analysis revealed a positive association between positive responses to TSGB and KIT (OR 7.004, 95% CI 1.26–39.02). Among 32 patients who underwent SCS implantation, 68.8% (n = 22) experienced short-term effectiveness. Positive response to TSGB was significantly higher in SCS responders (45.5%, n = 10) than in non-responders (0.0%). However, there were no associations between pain reduction post-TSGB and that post-KIT or post-SCS.

Conclusions
A positive response to TSGB is a potential predictor for positive KIT and SCS outcomes among patients with chronic upper-extremity pain, including CRPS.
Toward Realistic Models in T2/ℤ2 Flux Compactification
Authors: Imai Hiroki , Maru Nobuhito 
Published: May 2024

Abstract
We consider a six-dimensional gauge theory compactified on $T^2/\mathbb {Z}_2$ with magnetic flux. The configurations of models are classified by winding numbers at the fixed points. Requiring the existence of generation numbers and Yukawa coupling, we see that allowed and forbidden configurations are described by geometry of winding numbers.
The role of thematic specialization in international scientific collaboration: the case of Chagas research
Authors: López Emanuel , Arza Valeria 
Published: May 2024

Abstract
Scientists are increasingly specializing to manage the vast knowledge bases within their domains, making knowledge-sharing strategies essential for scientific progress. Among these strategies, international scientific collaboration has increased markedly. We analyse the role of thematic specialization as one of its drivers in the context of Chagas research, a neglected disease. Since there is a strong research tradition in Latin America on this topic, it is an interesting context to study centre-periphery research dynamics. Using a novel bibliometric database on Chagas research during 1990–2019, we exploit its panel structure in a gravity-type econometric model. The findings show that the degree of specialization and its proximity among partners boost joint publications. Both effects are particularly important for country dyads involving authors from the periphery linked to authors in the centre. This paper is the first empirical study to assess the role of specialization as a driver of international scientific collaboration.
The risk of sexual dysfunction in Chinese women with recurrent pregnancy loss and the associated factors: a multicenter cross-sectional study
Authors: Liu Chuanjiao , Zhang Yiduo , Xu Qian , Yang Chen , Tang Yuanting , Zhang Qiong , Liu Xiumei , Yue Yangang , Yu Fan 
Published: May 2024

Abstract

Background
Recurrent pregnancy loss (RPL) is a severe traumatic event for women of childbearing age. However, the association between RPL and female sexual dysfunction was unknown.

Aim
The study sought to investigate the association between RPL and sexual dysfunction, and to explore the risk factors of sexual dysfunction for RPL patients.

Methods
A multicenter cross-sectional study involving both RPL patients and healthy women was performed in 3 different hospitals in West China from May 2021 to January 2023. Baseline information including sociodemographic data and disease histories were collected. The Female Sexual Function Index (FSFI) was used to assess the sexual function of participants.

Outcomes
The main outcome was the proportion of women at increased risk of sexual dysfunction (total FSFI scores &lt;26.55), and the secondary outcome was risk factors of sexual dysfunction in RPL patients.

Results
A total of 233 RPL patients and 185 healthy women were included in this study. RPL patients had significantly lower total FSFI scores (median 31.7 [interquartile range, 26.6-33.5] vs 33.0 [interquartile range, 31.2-34.1]; P &lt; .001) and a significantly higher risk of sexual dysfunction than healthy women (24.9% vs 8.6%; P &lt; .001). Body mass index &gt;24 kg/m2 (adjusted odds ratio [OR], 4.132; 95% confidence interval [CI], 1.902-8.976, P &lt; .001), working &gt;8 h/d (adjusted OR, 2.111; 95% CI, 1.020-4.369, P = .044), and unexplained RPL (adjusted OR, 3.785; 95% CI, 1.967-7.280, P &lt; .001) were independent risk factors of sexual dysfunction for RPL patients.

Clinical Implications
RPL patients, especially those patients with the previously mentioned risk factors, should be focused on the risk of sexual dysfunction, and appropriate preventions could be applied.

Strength and Limitations
We explored the association between RPL and sexual dysfunction and explored the risk factors of sexual dysfunction among RPL patients for the first time, and the multicenter data increased the generalizability of results. However, the cross-sectional design did not provide an exact causal relationship between RPL and sexual dysfunction, and potential risk factors related to mental health were not investigated.

Conclusion
RPL patients were at an increased risk of sexual dysfunction. Overweight, fatigue caused by work, and unexplained RPL were risk factors of sexual dysfunction for RPL patients.
Lead-induced liver fibrosis and inflammation in mice by the AMPK/MAPKs/NF-κB and STAT3/TGF-β1/Smad2/3 pathways: the role of Isochlorogenic acid a
Authors: Guo Jun-Tao , Li Han-Yu , Cheng Chao , Shi Jia-Xue , Ruan Hai-Nan , Li Jun , Liu Chan-Min 
Published: May 2024

Abstract
Lead (Pb) is a nonessential heavy metal, which can cause many health problems. Isochlorogenic acid A (ICAA), a phenolic acid present in tea, fruits, vegetables, coffee, plant-based food products, and various medicinal plants, exerts multiple effects, including anti-oxidant, antiviral, anti-inflammatory and antifibrotic functions. Thus, the purpose of our study was to determine if ICAA could prevent Pb-induced hepatotoxicity in ICR mice. An evaluation was performed on oxidative stress, inflammation and fibrosis, and related signaling. The results indicate that ICAA attenuates Pb-induced abnormal liver function. ICAA reduced liver fibrosis, inflammation and oxidative stress caused by Pb. ICAA abated Pb-induced fibrosis and decreased inflammatory cytokines interleukin-1β (IL-1β) and tumor necrosis factor-alpha (TNF-α). ICAA abrogated reductions in activities of superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx). Masson staining revealed that ICAA reduced collagen fiber deposition in Pb-induced fibrotic livers. Western blot and immunohistochemistry analyses showed ICAA increased phosphorylated AMP-activated protein kinase (p-AMPK) expression. ICAA also reduced the expression of collagen I, α-smooth muscle actin (α-SMA), phosphorylated extracellular signal-regulated kinase (p-ERK), phosphorylated c-jun N-terminal kinase (p-JNK), p-p38, phosphorylated signal transducer and phosphorylated activator of transcription 3 (p-STAT3), transforming growth factor β1 (TGF-β1), and p-Smad2/3 in livers of mice. Overall, ICAA ameliorates Pb-induced hepatitis and fibrosis by inhibiting the AMPK/MAPKs/NF-κB and STAT3/TGF-β1/Smad2/3 pathways.
The performance and costs of XTT, resazurin, MTS and luciferin as viability dyes in in vitro susceptibility testing of Madurella mycetomatis
Authors: Ma Jingyi , Eadie Kimberly , Fahal Ahmed , Verbon Annelies , van de Sande Wendy W J 
Published: May 2024

Abstract

Background
in vitro susceptibility testing for the non-sporulating fungus Madurella mycetomatis is performed with a hyphal suspension as starting inoculum and a viability dye for endpoint reading. Here we compared the performance of four different viability dyes for their use in in vitro susceptibility testing of M. mycetomatis.

Methods
To compare the reproducibility and the agreement between the viability dyes 2,3-bis-(2-methoxy-4-nitro-5-sulfphenyl)-2H-tetrazolium-5-carboxanilide salt (XTT), resazurin, 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium salt (MTS) and luciferin, the in vitro susceptibilities of 14 genetically diverse M. mycetomatis isolates were determined for itraconazole and amphotericin B. The reproducibility, agreement, price and ease of use were compared.

Results
Each of the four dyes gave highly reproducible results with &gt;85.7% reproducibility. Percentage agreement ranged between 78.9% and 92.9%. Resazurin was the most economical to use (0.0009 €/minimal inhibitory concentration [MIC]) and could be followed in real time. Luciferin omitted the need to transfer the supernatant to a new 96-well plate, but cost 6.07 €/MIC.

Conclusion
All four viability dyes were suitable to determine the in vitro susceptibility of M. mycetomatis against itraconazole and amphotericin B. Based on the high reproducibility, high percentage agreement, price and possibility to monitor in real time, resazurin was the most suited for routine in vitro susceptibility testing in the diagnostic laboratory in mycetoma-endemic countries. Because luminescence could be measured directly without the need to transfer the supernatant to a new 96-well plate, luciferin is suitable for drug-screening campaigns.

Lay summary
To determine the in vitro susceptibility testing in the non-sporulating fungus Madurella mycetomatis, a viability dye is needed for endpoint reading. In this study we tested the viability dyes XTT, resazurin, MTS and luciferin for their use in in vitro susceptibility testing. It appeared that they all could be used but there were differences in time to result and costs associated with them.
Fungal Communities Shift with Soybean Cyst Nematode Abundance in Soils
Authors: López Melanie Medina , Lopez-Nicora Horacio D. , Ponce Maria Soledad Benitez 
Published: May 2024

Plant parasitic nematodes pose a significant threat to food security. Management strategies for these pathogens are limited, and additional tools for their control are needed. Some fungi have shown promise for biocontrol; however, their success in the field has varied. In contrast, some fungal plant pathogens form synergistic associations with nematodes, resulting in increased plant disease severity. However, how the two groups of fungi change with different plant parasitic nematode abundances in the soil is underexplored. In this study, we used the soybean cyst nematode as a model to understand these changes. We sampled soil from 171 Ohio soybean fields in 2019 and 2021 and determined soybean cyst nematode abundance. We identified the fungi in the samples through amplicon sequencing of the 18S-ITS rDNA regions. Edaphoclimatic factors were used to classify samples into geographic regions to account for environmental differences between sampling locations. We hypothesized that fungal communities would be influenced by both region and soybean cyst nematode abundance. K-near-neighbor analysis revealed that fungal communities follow regional patterns. We also found that soybean cyst nematode abundance was associated with changes in these communities regardless of the region. Two potential nematophagous fungi were found to be prevalent in Ohio through core community analysis, although they were enriched when soybean cyst nematode abundance was high. Finally, differential network analysis showed that interactions among fungal community members change when soybean cyst nematode is present in the soil. Together these results suggest that this nematode significantly shifts the fungal community composition in field soils. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
Cultivars and Production Environments Shape Shoot Endophyte Profiles of Boxwood with Different Blight Resistance
Authors: Li Xiaoping , Weiland Jerry E. , Ohkura Mana , Luster Douglas G. , Daughtrey Margery L. , Gouker Fred E. , Chen Gloria , Kong Ping , Hong Chuanxue 
Published: May 2024

Phyllosphere colonizers, including bacteria and fungi, are critical for plant growth and health. However, how they are affected simultaneously by the host plant cultivar, local environment, and agricultural practices was not well understood. We used boxwood, an iconic landscape plant and a major evergreen shrub crop in the United States nursery industry, as a model plant and sequenced 16S rRNA and ITS amplicons to examine the assemblages of endophytic bacteria and fungi in the shoots of four cultivars representing three levels of boxwood blight resistance under two distinct climates and production systems in Oregon and Virginia. Cultivar and local environment were the two main drivers shaping the composition and structure of the boxwood endophytic microbial community, particularly the fungal community. Three bacterial and seven fungal genera were consistently identified with high prevalence and abundance as the core taxa from four cultivars and two locations across three sampling times. The microbial composition varied among the levels of boxwood blight resistance, and taxa specific to the tolerant cultivar were fewer compared to the susceptible one. Identification of these microbial indicators, along with the core taxa, is foundational for developing a microbiome-based plant breeding program and a systems approach to improve boxwood health and production under a changing climate. [Formula: see text] Copyright © 2024 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
IL1β‐NFκβ‐Myocardin signaling axis governs trophoblast‐directed plasticity of vascular smooth muscle cells
Authors: Das Priyanka , Bose Rumela , Paul Madhurima , Nandy Debdyuti , Basak Trishita , Ain Rupasri 
Published: May 2024

AbstractVascular smooth muscle cell (VSMC) plasticity is fundamental in uterine spiral artery remodeling during placentation in Eutherian mammals. Our previous work showed that the invasion of trophoblast cells into uterine myometrium coincides with a phenotypic change of VSMCs. Here, we elucidate the mechanism by which trophoblast cells confer VSMC plasticity. Analysis of genetic markers on E13.5, E16.5, and E19.5 in the rat metrial gland, the entry point of uterine arteries, revealed that trophoblast invasion is associated with downregulation of MYOCARDIN, α‐smooth muscle actin, and calponin1, and concomitant upregulation of Smemb in VSMCs. Myocardin overexpression or knockdown in VSMCs led to upregulation or downregulation of contractile markers, respectively. Co‐culture of trophoblast cells with VSMCs decreased MYOCARDIN expression along with compromised expression of contractile markers in VSMCs. However, co‐culture of trophoblast cells with VSMCs overexpressing MYOCARDIN inhibited their change in phenotype, whereas, overexpression of transactivation domain deleted MYOCARDIN failed to elicit this response. Furthermore, the co‐culture of trophoblast cells with VSMCs led to the activation of NFκβ signaling. Interestingly, despite producing IL‐1β, trophoblast cells possess only the decoy receptor, whereas, VSMCs possess the IL‐1β signaling receptor. Treatment of VSMCs with exogenous IL‐1β led to a decrease in MYOCARDIN and an increase in phosphorylation of NFκβ. The effect of trophoblast cells in the downregulation of MYOCARDIN in VSMCs was reversed by blocking NFκβ translocation to the nucleus. Together, these data highlight that trophoblast cells direct VSMC plasticity, and trophoblast‐derived IL‐1β is a key player in downregulating MYOCARDIN via the NFκβ signaling pathway.