bluuebunny/crossref_metadata_2025_split huggingface dataset:
Therapist fidelity with an exposure‐based treatment of ptsd in adults with schizophrenia or schizoaffective disorder
Authors: Long Mary E. , Grubaugh Anouk L. , Elhai Jon D. , Cusack Karen J. , Knapp Rebecca , Frueh B. Christopher 
Published: January 2010

AbstractThis study examined therapists' fidelity to a manualized, multicomponent cognitive‐behavioral intervention for posttraumatic stress disorder (PTSD), including exposure therapy, among public sector patients with a psychotic disorder. Independent raters assessed therapists' competence and adherence, rating 20% of randomly selected audio taped sessions (n=57 sessions, coded by two raters, with strong interrater agreement). Adherence ratings indicated that therapists complied well with the protocol, and competency ratings typically averaged “very good” or higher (6 on 7‐point Likert scale). Findings suggest that therapists can effectively deliver a manualized cognitive‐behavioral intervention for PTSD, with exposure therapy, to patients with severe mental illness without compromise to the structure of sessions and/or the therapeutic relationship. © 2010 Wiley Periodicals, Inc. J Clin Psychol 66:1–11, 2010.
Role of hypoxia and autophagy in MDA‐MB‐231 invasiveness
Authors: Indelicato Manuela , Pucci Bruna , Schito Luana , Reali Valentina , Aventaggiato Michele , Mazzarino Maria C. , Stivala Franca , Fini Massimo , Russo Matteo A. , Tafani Marco 
Published: January 2010

AbstractSurvival strategies adopted by tumor cells in response to a hypoxic stress include activation of hypoxia‐inducible factor 1 (HIF‐1) and autophagy. However, the importance and the function of each molecular response is not well defined. In the present study, we investigated invasiveness, migration, matrix metalloproteinases (MMPs) activity, and cell survival of MDA‐MB‐231 cells under normoxia, hypoxia, and hypoxia/reoxygenation (H/R). Moreover, to assess the importance of hypoxia and autophagy on the parameters studied, cells were either left untreated or treated with Chetomin (a selective inhibitor of HIF‐1α) or trifluoperazine (TFP, an activator of autophagy). We found that hypoxia and H/R stimulated invasiveness and migration of MDA‐MB‐231 cells with an increased MMP‐2 activity. Chetomin and TFP differently regulated the cellular behavior under the oxygenation conditions studied. In fact, Chetomin was most effective in inhibiting cell invasion, MMPs activity, and cell survival under hypoxia but not normoxia or H/R. By contrast, TFP inhibition of cell invasion, migration, and cell survival was independent from oxygenation conditions. TFP‐induced autophagy was inhibited by light chain protein 3 (LC3) silencing or 3‐methyladenine (3MA) treatment. In fact, LC3‐silenced cells were able to invade in the presence of TFP without any GATE16 processing and p62 degradation. Immunofluorescence assay showed that LC3 silencing inhibited TFP‐induced autophagosome formation. However, we also showed that both TPF treatment and LC3 silencing caused cytoskeleton impairments suggesting a possible interaction between LC3 and cytoskeleton components. In conclusion, our study shows that hypoxia and autophagy by acting on common (HIF‐1α) or separate (MMPs, cytoskeleton) targets differently regulate cell invasion, MMPs activity, and survival. J. Cell. Physiol. 223: 359–368, 2010. © 2010 Wiley‐Liss, Inc.
Evidence for organ‐specific stem cell microenvironments
Authors: Ghinassi Barbara , Martelli Fabrizio , Verrucci Maria , D'Amore Emanuela , Migliaccio Giovanni , Vannucchi Alessandro Maria , Hoffman Ronald , Migliaccio Anna Rita 
Published: January 2010

AbstractThe X‐linked Gata1low mutation in mice induces strain‐restricted myeloproliferative disorders characterized by extramedullary hematopoiesis in spleen (CD1 and DBA/2) and liver (CD1 only). To assess the role of the microenvironment in establishing this myeloproliferative trait, progenitor cell compartments of spleen and marrow from wild‐type and Gata1low mice were compared. Phenotype and clonal assay of non‐fractionated cells indicated that Gata1low mice contain progenitor cell numbers 4‐fold lower and 10‐fold higher than normal in marrow and spleen, respectively. However, progenitor cells prospectively isolated from spleen, but not from marrow, of Gata1low mice expressed colony‐forming function in vitro. Therefore, calculation of cloning activity of purified cells demonstrated that the total number of Gata1low progenitor cells was 10‐ to 100‐fold lower than normal in marrow and >1,000 times higher than normal in spleen. This observation indicates that Gata1low hematopoiesis is favored by the spleen and is in agreement with our previous report that removal of this organ induces wild‐type hematopoiesis in heterozygous Gata1low/+ females (Migliaccio et al., 2009, Blood 114:2107). To clarify if rescue of wild‐type hematopoiesis by splenectomy prevented extramedullary hematopoiesis in liver, marrow cytokine expression profile and liver histopathology of splenectomized Gata1low/+ females were investigated. After splenectomy, the marrow expression levels of TGF‐β, VEGF, osteocalcin, PDGF‐α, and SDF‐1 remained abnormally high while Gata1low hematopoiesis was detectable in liver of both CD1 and DBA/2 mutants. Therefore, in the absence of the spleen, Gata1low hematopoiesis is supported by the liver suggesting that treatment of myelofibrosis in these animals requires the rescue of both stem cell and microenvironmental functions. J. Cell. Physiol. 223: 460–470, 2010. © 2010 Wiley‐Liss, Inc.
Retinoic acid‐metabolizing enzyme cytochrome P450 26a1 (cyp26a1) is essential for implantation: Functional study of its role in early pregnancy
Authors: Han Bing‐Chen , Xia Hong‐Fei , Sun Jing , Yang Ying , Peng Jing‐Pian 
Published: January 2010

AbstractVitamin A (VA) is required for normal fetal development and successful pregnancy. Excessive VA intake during pregnancy may lead to adverse maternal and fetal effects. Cytochrome P450 26A1 (cyp26a1), a retinoic acid (RA)‐metabolizing enzyme, is involved in VA metabolism. It has been shown that cyp26a1 is expressed in female reproductive tract, especially in uterus. In order to investigate the role of cyp26a1 during pregnancy, we constructed a recombinant plasmid DNA vaccine encoding cyp26a1 protein and immunized mice with the plasmid. Compared to control groups, the pregnancy rate of the cyp26a1 plasmid‐immunized mice were significantly decreased (P < 0.01). Further results showed that both cyp26a1 mRNA and protein were specifically induced in the uterus during implantation period and localized in the uterine luminal epithelium. Importantly, the number of implantation sites was also significantly reduced (P < 0.05) after the uterine injection of cyp26a1‐specific antisense oligos or anti‐cyp26a1 antibody on day 3 of pregnancy. Accordingly, the expression of RA‐related cellular retinoic acid binding protein 1 and tissue transglutaminase was markedly increased (P < 0.05) in the uterine luminal epithelium after intrauterine injection treatments. These data demonstrate that uterine cyp26a1 activity is important for the maintenance of pregnancy, especially during the process of blastocyst implantation. J. Cell. Physiol. 223: 471–479, 2010. © 2010 Wiley‐Liss, Inc.
MicroRNAs as gatekeepers of apoptosis
Authors: Subramanian Subbaya , Steer Clifford J. 
Published: January 2010

AbstractApoptosis is a well‐orchestrated cellular mechanism that balances the effects of cell proliferation and cell death. MicroRNAs (miRNAs) have been shown to control cell growth, differentiation, and apoptosis; and can be significantly deregulated in many cancers types. In fact, the ability to evade apoptosis is a hallmark of tumorigenesis. Although the role of miRNAs in the regulation of apoptosis is not fully understood, the recent influx of data strongly suggests that miRNAs play a significant role in regulating programmed cell death, or apoptosis. The genes involved in apoptotic pathways can be broadly classified as pro‐apoptotic and anti‐apoptotic. Many of these apoptotic genes, irrespective of their positive or negative functional role in apoptosis, are regulated by miRNAs. In this review, we discuss the emerging role of miRNA‐mediated gene networks in the control of apoptosis. J. Cell. Physiol. 223: 289–298, 2010. © 2010 Wiley‐Liss, Inc.
Methoxyl groups of lignin are essential carbon donors in C1 metabolism of Sphingobium sp. SYK‐6
Authors: Sonoki Tomonori , Masai Eiji , Sato Kanna , Kajita Shinya , Katayama Yoshihiro 
Published: January 2010

AbstractSphingobium sp. SYK‐6 can utilize lignin biphenyl compounds, such as 2,2′‐dihydroxy‐3,3′‐dimethoxy‐5,5′‐dicarboxybiphenyl (DDVA). In the metabolism of DDVA, this microorganism exploits two O ‐demethylation systems with different substrate specificities, namely, a DDVA‐specific oxygenative O ‐demethylation system and a vanillate‐specific (VA‐specific) tetrahydrofolate‐dependent (THF‐dependent) methyltransferase system. We examined the way in which these two systems interact in the metabolism of lignin in Sphingobium sp. SYK‐6. Our results indicate that THF accepts methyl groups derived not only from the THF‐dependent O ‐demethylation of VA but also from the oxygenative O ‐demethylation of DDVA. Thus, the methoxyl groups of lignin model compounds are an essential source of carbon in Sphingobium sp. SYK‐6. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Cloning of a novel ovalbumin gene from quail oviduct and its heterologous expression in Pichia pastoris
Authors: Yang Shaohui , Chen Gong , Yu Xinchun , Li Minggang , Wang Jiehua 
Published: January 2010

AbstractAn ovalbumin gene was cloned from Chinese quail (Coturnix coturnix) oviduct by RT‐PCR and then inserted into the P. pastoris genome under the control of the methanol inducible 5′ alcohol oxidase (AOX) promoter. The recombinant P. pastoris strain was demonstrated to be able to efficiently secrete quail ovalbumin by ELISA analysis using a polyclonal antibody raised against quail ovalbumin. The results showed that induction by 0.75% methanol for 48 h led to the synthesis of secreted quail ovalbumin up to a yield of 5.45 g l–1. The recombinant ovalbumin was further purified into homogeneity through ion exchange and gel filtration chromatography and SDS‐PAGE analysis revealed that, compared to natural ovalbumin, the recombinant ovalbumin could have been glycosylated to the similar extent by P. pastoris. (© 2009 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Moment analysis of chromatographic behavior in reversed‐phase liquid chromatography
Authors: Miyabe Kanji 
Published: January 2010

AbstractChromatographic behavior, such as sample retention, band broadening, elution peak profile, column efficiency, separation performance, and so on, depends on both the retention equilibrium and mass transfer kinetics in the column. A great number of research works have been carried out so far on the retention equilibrium in chromatography. However, chromatographic behavior has not so abundantly been investigated from kinetic points of view because of some essential difficulties of kinetic study. Additionally, in contrast with the extensive applications of various HPLC instruments and separation media developed in recent decades, it seems that arrangements of theoretical bases and experimental strategies relating to the kinetic study are not well recognized by most chromatographers. In this review, some items of information about the moment analysis method and concrete examples about mass transfer kinetics derived by the method are provided. The progress of some strategies for the kinetic study on chromatography is also introduced.
Comprehensive two dimensional gas chromatography review
Authors: Cortes Hernan J. , Winniford Bill , Luong Jim , Pursch Matthias 
Published: January 2010

AbstractAdvances, developments and applications in 2‐D comprehensive GC (GC×GC) from 2007 through October 2008 are reviewed, with emphasis on modulation, and data handling, and applications of current relevance. Industrial perspectives as well as suggestions where further developments would be beneficial are summarized in the concluding remarks.
Genuineness assessment of mandarin essential oils employing gas chromatography‐combustion‐isotope ratio MS (GC‐C‐IRMS)
Authors: Schipilliti Luisa , Tranchida Peter Quinto , Sciarrone Danilo , Russo Marina , Dugo Paola , Dugo Giovanni , Mondello Luigi 
Published: January 2010

AbstractCold‐pressed mandarin essential oils are products of great economic importance in many parts of the world and are used in perfumery, as well as in food products. Reconstituted mandarin oils are easy to find on the market; useful information on essential oil authenticity, quality, extraction technique, geographic origin and biogenesis can be attained through high‐resolution GC of the volatile fraction, or enantioselective GC, using different chiral stationary phases. Stable isotope ratio analysis has gained considerable interest for the unveiling of citrus oil adulteration, detecting small differences in the isotopic carbon composition and providing plenty of information concerning the discrimination among products of different geographical origin and the adulteration of natural essential oils with synthetic or natural compounds. In the present research, the authenticity of several mandarin essential oils was assessed through the employment of GC hyphenated to isotope ratio MS, conventional GC flame ionization detector, enantioselective GC and HPLC. Commercial mandarin oils and industrial natural (declared as such) mandarin essential oils, characterized by different harvest periods and geographic origins, were subjected to analysis. The results attained were compared with those of genuine cold‐pressed Italian mandarin oils, obtained during the 2008–2009 harvest season.
Rapid identification and preparative isolation of antioxidant components in licorice
Authors: Sil Lee Yeon , Ha Kim Seon , Kyu Kim Jin , Shin Hyun‐Kyung , Kang Young‐Hee , Yoon Park Jung Han , Lim Soon Sung 
Published: January 2010

AbstractThis study employed the online HPLC‐2,2′‐azinobis‐(3‐ethylbenzothiazoline‐6‐sulfonate radical cation (ABTS+·) bioassay to rapidly determine antioxidant compounds occurring in the licorice extract of Glycyrrhiza uralensis. The negative peaks of the ABTS+· radical scavenging detection system, which indicated the presence of antioxidant activity, were monitored by measuring the decrease in absorbance at 734 nm. The ABTS+‐based antioxidant activity profile showed that three peaks exhibited antioxidant activity, and then the high‐speed counter‐current chromatography technique of preparative scale was successfully applied to separate the three peaks I‐III in one step from the licorice extract. The high‐speed counter‐current chromatography was performed using a two‐phase solvent system composed of n‐hexane–ethyl acetate–methanol–water (6.5:5.5:6:4, v/v). Yields of the three peaks, dehydroglyasperin C (I, 95.1% purity), dehydroglyasperin D (II, 96.2% purity), and isoangustone A (III, 99.5% purity), obtained were 10.33, 10.43, and 6.7% respectively. Chemical structures of the purified dehydroglyasperin C (I), dehydroglyasperin D (II), and isoangustone A (III) were identified by ESI‐MS and 1H‐ and 13C‐NMR analysis.
Fabrication and characterisation of capillary polymeric monoliths incorporating continuous stationary phase gradients
Authors: Currivan Sinead , Connolly Damian , Gillespie Eoin , Paull Brett 
Published: January 2010

AbstractPolymeric monoliths in capillary formats have been fabricated incorporating a gradient of charged functional groups along their length. Scanning capacitively coupled contactless conductivity detection (sC4D) was then used to measure the conductive response of the stationary phase and characterise the relative axial distribution of functional groups along the column length. Gradients of 2‐acrylamido‐2‐methyl‐1‐propanesulphonic acid were prepared using either photografting methods or by filling a capillary column with segmented plugs of monomer mixtures each containing incrementally higher concentrations of the functional monomer. The utility of sC4D as a rapid and non‐invasive tool for assessing the slope of a variety of gradient configurations is demonstrated. Repeatability of the sC4D measurements was <1.7% RSD. Columns with a gradient of covalently bonded iminodiacetic acid were also produced. Changes in the gradient slope were observed after chelation of copper on the stationary phase via a reduction of the conductive response. The effect upon the observed gradient profile of changing the co‐monomer composition during column fabrication was studied.
Media richness and internet exploration
Authors: Jacob Céline , Guéguen Nicolas , Petr Christine 
Published: January 2010

AbstractPrevious research found that media richness is associated with positive evaluation of a web site. An experiment was carried out where participants were instructed to explore a web site presenting a popular town: Venice. According to a random distribution, participants were exposed (or not) to street‐sounds when exploring the web site and had the possibility to control (or not) the photography (using a 360° navigation with their mouse) displayed on the web site. Results shown that higher evaluation were associated with sounds and mouse‐control. Theory of media richness was used to explain our results and the managerial application for tourism was discussed. Copyright © 2010 John Wiley & Sons, Ltd.
Laser in der Halbleiterbearbeitung
Authors: Haupt Oliver , Kling Rainer 
Published: January 2010

AbstractDer Halbleitermarkt wird im Wesentlichen von kristallinem Silizium als Basiswerkstoff dominiert. Der stetige Trend zur Verringerung der Waferdicke stellt hierbei steigende Anforderungen an die Qualität der Bearbeitungsprozesse, da bei dünneren Wafern das Bruchrisiko erheblich zunimmt. Kristallines Silizium ist aufgrund des sprödbrüchigen Verhaltens hochsensibel für thermische Prozesse, dennoch ist durch den Einsatz von geeigneter Laserstrahlung ein berührungsloses Bearbeiten möglich. Für die Nutzung von leistungsstärkeren Laserstrahlquellen wird zukünftig im Hinblick auf die Bearbeitung gro�er Flächen bei kurzen Prozesszeiten eine massive Prozessparallelisierung notwendig sein.
Der Laser, Schlüsseltechnologie für moderne Halbleiterproduktion
Authors: Fischer Gabriele 
Published: January 2010

AbstractDie Einsatzgebiete des Lasers in der Halbleiterproduktion sind vielfältig. Angefangen mit dem Markieren und Ritzen der Wafer über das Beschriften und Vereinzeln der fertigen Bausteine bis zur Fehleranalyse. Da neben Silizium, metallische Werkstoffe sowie Kunststoffe, insbesondere die Epoxydharze der Vergussmaterialien bearbeitet werden, setzt die Entwicklung einer optimalen Lösung eine entsprechende Auswahl an Strahlquellen voraus.Dioden‐seitengepumpte und diodenendgepumpten Festkörperlasern, Faserlasen, Scheiben‐ und CO2‐Lasern gehören zum Spektrum der relevanten Strahlquellen für die Halbleiterfertigung.
The Continuation of Moore's Law
Authors: Brown Daniel , Farrar Nigel 
Published: January 2010

AbstractMoore's Law, which predicts thecontinued increase in device density onintegrated circuits, has been driven by advancesin lithography. Both resolutionand productivity of the exposure toolhave been enabled by the light sourcetechnology. The transition to shorterwavelength, first by introducing excimerlasers and more recently by developmentof EUV Laser Produced Plasma sources hasdriven resolution. In addition, the continuedcost effectiveness of lithography isdependent on the scaling of the power
output for both types of source.
Drei Global Player im Gespräch
Authors: Mertin Michael , Ambroseo John R. , Braun Günther 
Published: January 2010

AbstractMehrere Marktexperten rechnen im Krisenjahr 2009 mit Umsatzeinbrüchen von bis zu 40 Prozent in der Lasermaterialbearbeitungsbranche. Francisco Velasco und Andreas Tho� sprachen mit drei führenden Managern aus der Laserindustrie über Chancen und Strategien in der Krise.
Novel Poly(amidoamine)s with Pendant Primary Amines as Highly Efficient Gene Delivery Vectors
Authors: Liu Min , Chen Jun , Cheng Yan‐Ping , Xue Ya‐Nan , Zhuo Ren‐Xi , Huang Shi‐Wen 
Published: January 2010

AbstractThree poly(amidoamine)s with pendant primary amines were synthesized by Michael polyaddition of a diamine to N,N‐methylenebis(acrylamide). The poly(amidoamine)s with primary and tertiary amines possess a high buffer capacity between pH = 5 and 7, which facilitates the escape of polymer/DNA complexes from endosomes according to the ‘proton sponge hypothesis’. In vitro cytotoxicity of poly(amidoamine)s depends on the side‐chain structure of the polymer. Polymers 1a–1c can condense plasmid DNA by electrostatic interactions to form nanosized polyelectrolyte complexes with a positive surface charge. In vitro transfection results indicate that the transfection efficiency of polymer 1b under optimized conditions is comparable to that of commercial branched PEI 25 000.magnified image
Aligned Bioactive Multi‐Component Nanofibrous Nanocomposite Scaffolds for Bone Tissue Engineering
Authors: Jose Moncy V. , Thomas Vinoy , Xu Yuanyuan , Bellis Susan , Nyairo Elijah , Dean Derrick 
Published: January 2010

AbstractThe ability to mimic the chemical, physical and mechanical properties of the natural extra‐cellular matrix is a key requirement for tissue engineering scaffolds to be successful. In this study, we successfully fabricated aligned nanofibrous multi‐component scaffolds for bone tissue engineering using electrospinning. The chemical features were mimicked by using the natural components of bone: collagen and nano‐hydroxyapatite along with poly[(D,L‐lactide)‐co‐glycolide] as the major component. Anisotropic features were mimicked by aligning the nanofibers using a rotating mandrel collector. We evaluated the effect of incorporation of nano‐HA particles to the system. The morphology and mechanical properties revealed that,at low concentrations, nano‐HA acted as a reinforcement. However, at higher nano‐HA loadings, it was difficult to disrupt aggregations and, hence, a detrimental effect was observed on the overall scaffold properties. Thermal analysis showed that there were slight interactions between the individual components even though the polymers existed as a two‐phase system. Preliminary in vitro cell‐culture studies revealed that the scaffold supported cell adhesion and spreading. The cells assumed a highly aligned morphology along the direction of fiber orientation. Protein adsorption experiments revealed that the synergistic effect of increased surface area and the presence of nano‐HA in the polymer matrix enhanced total protein adsorption. Crosslinking with 1‐ethyl‐3‐(3‐dimethylaminopropyl) carbodiimide hydrochloride resulted in improved mechanical properties of the scaffolds and improved degradation stability, under physiological conditions.magnified image