bluuebunny/crossref_metadata_2025_split huggingface dataset:
A GOG 210 aCGH study of gain at 1q23 in endometrioid endometrial cancer in the context of racial disparity and outcome
Authors: Morrison Carl , Miecznikowski Jeffrey , Darcy Kathleen M. , Dolce Jean M. , Kandel Eugene , Erwin Deborah O. , Liu Song , Shepherd Lori , Cohn David , McMeekin D. Scott , Block AnneMarie W. , Nowak Norma J. , Maxwell Larry 
Published: May 2010

AbstractThe goal of this study was to identify recurrent regions of genomic gain or loss in endometrial cancer of the endometrioid type in the context of racial disparities in mortality for this disease. Array comparative genomic hybridization (aCGH) analysis was performed on 80 frozen primary tumors from the Gynecologic Oncology Group (GOG)‐210 bank using the RPCI 19K BAC arrays. The 80 patients included 20 African American (AA) Stage I, 20 White (W) Stage I, 20 African American (AA) Stage IIIC/IV, and 20 White (W) Stage IIIC/IV. A separate subset of 220 endometrial cancers with outcome data was used for validation. A 1.6‐Mbp region of gain at 1q23 was identified by aCGH in all AA patients and high grade W patients, but not W low grade patients. In the validation arm of 220 patients copy number gain at this region was validated using FISH and locus specific BACs. The number of AA patients in the validation arm was too small to confirm the aCGH association with racial disparity. Kaplan‐Meier curves for survival showed a significant difference for gain at 1q23 versus no gain (log rank P = 0.0014). When subdivided into various groups of risk by stage and grade the survival curves showed a decreased survival for high grade and/or stage tumors, but not for low grade and/or stage endometrioid tumors. Univariate analyses for gain at 1q23 showed a significant association (P = 0.009) with survival. Multivariate analysis for gain at 1q23 did not show a significant association with survival (P = 0.14). © 2010 Wiley‐Liss, Inc.
Accuracy of whole‐body PET and PET‐CT in initial M staging of head and neck cancer: A meta‐analysis
Authors: Xu Guo‐Zeng , Zhu Xiao‐Dong , Li Ming‐Yao 
Published: May 2010

AbstractBackgroundA meta‐analysis was conducted to evaluate the accuracy of whole‐body positron emission tomography (PET) and PET‐CT in initial M staging of head and neck cancer.MethodsAfter a systematic review of English language studies, sensitivity, specificity, and other measures of whole‐body PET and PET‐CT were pooled. Summary receiver operating characteristic (SROC) curves were also used to summarize overall test performance.ResultsEight PET and 7 PET‐CT studies were identified. The pooled sensitivity estimates for PET and PET‐CT were 0.848 (95% confidence interval [CI], 0.776–0.905) and 0.875 (95% CI, 0.787–0.936). The pooled specificity estimates were 0.952 (95% CI, 0.933–0.967) and 0.950 (95% CI, 0.931–0.964). The Q* index estimates for PET‐CT (0.9409) were not significantly higher than for PET (0.9154; p > .05).ConclusionWhole‐body PET and PET‐CT have good diagnostic performance in initial M staging of head and neck cancer; although PET‐CT tends to have higher accuracy than PET. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Identifying risk factors for postoperative cardiovascular and respiratory complications after major oral cancer surgery
Authors: Dillon Jasjit K. , Liu Stanley Y. , Patel Chirag M. , Schmidt Brian L. 
Published: May 2010

AbstractBackgroundSurgical resection of oral cancer can be associated with significant postoperative cardiovascular and respiratory complications that require more sensitive predictors.MethodsAll patients with oral squamous cell carcinoma treated from July 2005 to April 2008 were retrospectively reviewed. The Goldman Revised Cardiac Risk Index (GRCRI) was used to predict cardiovascular complications. Other evidence‐based a priori predictors were applied in an h‐fold cross‐validation model.ResultsOperating room (OR) time was an independent predictor of cardiovascular complications (odds ratio = 1.54, p = .002, 95% confidence interval [CI] = 1.18–2.02) and respiratory complications (odds ratio = 1.3, p = .06, 95% CI = 0.99–1.64) after multivariate adjustment. OR time and estimated blood loss predicted cardiovascular complications with 73% sensitivity. The GRCRI achieved 37% sensitivity. OR time and tracheostomy predicted respiratory complications with 75% sensitivity.ConclusionsThe GRCRI was not prognostic for cardiovascular complications in patients with oral cancer. The most sensitive predictors for cardiovascular complications were OR time and estimated blood loss; for respiratory complications they were OR time and tracheostomy. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Role of core needle biopsy and ultrasonographic finding in management of indeterminate thyroid nodules
Authors: Park Kyung Tae , Ahn Soon‐Hyun , Mo Ji‐Hun , Park Young Joo , Park Do Joong , Choi Sang Il , Park So‐Yeon 
Published: May 2010

AbstractBackgroundThyroid fine‐needle aspiration (FNA) is used as a screening test of choice for evaluation of thyroid nodules. However, approximately 15% to 25% of the cases are classified as indeterminate, posing dilemmas in decision‐making. This study was designed to compare the diagnostic performances of second FNA and core needle biopsy of indeterminate nodules by initial FNA.MethodsFrom February 2005 through June 2009, 258 patients who completed scheduled follow‐ups were enrolled and the follow‐up results were analyzed.ResultsNondiagnostic results were obtained in 41.8% of the second FNA group and in 1.7% of the core needle biopsy group (p < .001; chi‐square). The nodules that show borderline features in preoperative ultrasonography had a malignancy rate of 18.3% and could be identified successfully with core needle biopsy.ConclusionCore needle biopsy is a better method for evaluating indeterminate nodules by initial FNA than second FNA, especially in patients with ultrasonographic findings of a borderline nodule. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Dual inhibition of epidermal growth factor receptor and insulin‐like growth factor receptor I: Reduction of angiogenesis and tumor growth in cutaneous squamous cell carcinoma
Authors: Galer Chad E. , Corey Christina L. , Wang Zhuoying , Younes Maher N. , Gomez‐Rivera Fernando , Jasser Samar A. , Ludwig Dale L. , El‐Naggar Adel K. , Weber Randal S. , Myers Jeffrey N. 
Published: May 2010

AbstractBackgroundCutaneous squamous cell carcinoma (CSCC) is the second most common nonmelanoma skin cancer. Most of the approximately 250,000 cases occurring annually in the United States are small, nonaggressive, and cured by excision alone. However, a subset of these tumors which are defined by poorly differentiated histology, large tumor size, invasion of adjacent structures, and/or regional metastases can prove resistant to treatment despite adjuvant radiotherapy and can have an increased risk of recurrence and nodal metastasis. Novel therapeutic approaches are necessary to improve the outcomes for patients with aggressive CSCC.MethodsWe analyzed the effect of targeted therapy on the growth and survival of CSCC cell lines using an anti‐insulin‐like growth factor‐I receptor (IGF‐IR) antibody, A12, alone or in combination with an anti‐epidermal growth factor receptor (EGFR) antibody, cetuximab, both in vitro and in vivo in an athymic nude mouse model of CSCC.ResultsTreatment with A12 and cetuximab inhibited the signaling pathways of IGF‐IR and EGFR and inhibited proliferation and induced apoptosis of squamous cell carcinoma (SCC) cell lines in vitro. Immunohistochemical staining revealed decreased proliferating cell nuclear antigen (PCNA), microvessel density, and increased apoptosis within the treated tumor xenografts. In addition, the administration of A12, alone or in combination with cetuximab inhibited the growth of tumors by 51% and 92%, respectively, and significantly enhanced survival in the nude mouse model of CSCC (p= .044 andp< .001, respectively).ConclusionThese data suggest that dual treatment with monoclonal antibodies to the EGFR and IGF‐IR may be therapeutically useful in the treatment of CSCC. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Field research on the performance of a rice husk envelope in a subsurface drainage system (case study Behshahr, Iran)
Authors: Ebrahimian Hamed , Parsinejad Massoud , Liaghat Abdolmajid , Akram Mojtaba 
Published: May 2010

AbstractThis study was conducted to evaluate the performance of subsurface drainage and a rice husk envelope in Behshahr, a coastal region in the northern part of Iran. The subsurface drainage system was monitored in the rainfall seasons of 2006 and 2007 (22 November to 19 March). Various parameters such as daily groundwater table fluctuations and drain discharge rate were measured. To evaluate the drainage envelope, the gradation curve, bulk density and natural durability of rice husk and also the head loss of the groundwater table in the vicinity of the drain were measured. The large difference between the approach‐flow head and entrance‐flow head could be related to clogging of the external part of the rice husk envelope. The overall conclusion of this study was that subsurface drainage system performance was not satisfactory due to poor control of groundwater table depth, which could be related to clogging of the rice husk drain envelope. Hooghoudt's equation is not recommended to be used for evaluation of design parameters in this case. The basic uncertainty in application of a rice husk envelope was its durability, which was found to be very variable and depended on soil environmental conditions. Copyright © 2010 John Wiley & Sons, Ltd.
Ribonucleoprotein immunoprecipitation (RNP‐IP): A direct in vivo analysis of microRNA‐targets
Authors: Hassan Mohammad Q. , Gordon Jonathan A.R. , Lian Jane B. , van Wijnen Andre J. , Stein Janet L. , Stein Gary S. 
Published: May 2010

AbstractWe have developed a novel Ribonucleoprotein Immunoprecipitation (RNP‐IP) method to isolate miR‐RISC complexes, associated microRNAs and target mRNAs. Our method characterizes mRNAs present in immunoprecipitates containing miR‐RISC complexes that were obtained using GW182 and AGO2 antibodies. MicroRNA bound transcripts were reverse transcribed and amplified using seed sequence and 3′UTR derived primers. This flexible IP‐based assay is a straightforward method to identify miRs participating in gene regulation and their cognate mRNAs in real time. J. Cell. Biochem. 110: 817–822, 2010. © 2010 Wiley‐Liss, Inc.
The effects of peptides with estrogen‐like activity on cell proliferation and energy metabolism in human derived vascular smooth muscle cells
Authors: Somjen D. , Knoll E. , Gayer B. , Kasher R. , Kohen F. , Stern N. 
Published: May 2010

AbstractHormone replacement therapy (HRT) for post‐menopausal symptoms in diabetes is associated with increased risk of coronary heart disease and stroke. Therefore, there is a need for new HRT with no adverse effects on diabetic post‐menopausal women. We developed peptides as potential estrogen mimetic compounds and now we evaluated the effects of the most efficacious peptide; hexapeptide estrogen‐mimetic peptide 1 (EMP‐1) (VSWFFE) in comparison to estrogen (E2) and peptides with weak activity A44 (KAWFFE) and A45 (KRAFFE) on modulation of cell proliferation of vascular smooth muscle cells (VSMC) growing in normal (ng) or high glucose (hg) concentrations. In ng EMP‐1‐like E2 inhibited cell proliferation at high concentration, and stimulated at low concentration. EMP‐1 did not affect E2 stimulation of DNA, but inhibited E2 inhibition of cell proliferation at high concentration. All effects by the combination of EMP‐1 and E2 were abolished at hg. A44‐stimulated cell proliferation at all concentrations and A45 had no effect. When A44 was co‐incubated with E2 at both concentrations, DNA synthesis was stimulated, but abolished at hg. A45 abolished E2 stimulation and inhibition of cell proliferation at both glucose concentrations. All peptides tested except A45‐stimulated CK‐specific activity at both glucose concentrations. In hg A44 stimulation of DNA was unaffected as well as its inhibition by EMP‐1. EMP‐1 and A44 similar to E2‐stimulated MAPK activity in ng or hg, suggesting similar mechanism of action. The results presented here suggest that EMP‐1 provided it acts similarly in vivo can replace E2 for treatment of post‐menopausal women in hyperglycemia due to diabetes. J. Cell. Biochem. 110: 1142–1146, 2010. Published 2010 Wiley‐Liss, Inc.
QSG‐7701 human hepatocytes form polarized acini in three‐dimensional culture
Authors: Zhang Fang , Xu Ren , Zhao Mu‐jun 
Published: May 2010

AbstractHepatocytes are polarized and fulfill a variety of liver‐specific functions in vivo; but the polarized tissue structure and many of these functions are lost when the cells are cultured on plastic. To recapitulate the polarized structure and tissue‐specific function of liver cells in culture, we established a three‐dimensional (3D) culture assay with the human hepatocyte line QSG‐7701. In 3D Matrigel culture, QSG‐7701 cells formed polarized spheroids with a center lumen, which is reminiscent of bile canaliculi in the liver. Immunofluoresence analysis showed that F‐actin bundles and radixin were mainly located at the apical membrane and that α6 and β1 integrins were localized basally in 3D culture. Lumen formation was associated with the selective apoptosis of centrally located cells and was accompanied by proliferative suppression during acinar development. Compared to QSG‐7701 cells in 2D or agarose gel cultures, the cells in 3D Matrigel culture maintained a given direction of biliary excretion and acquired higher levels of cytochrome P450 and albumin expression. Our study shows that the immortal human hepatocytes, QSG‐7701, in 3D Matrigel culture reacquire cardinal features of glandular epithelium in vivo, providing an ex vivo model to study liver‐specific function and tumorigenesis. J. Cell. Biochem. 110: 1175–1186, 2010. Published 2010 Wiley‐Liss, Inc.
Jmjd3 activates Mash1 gene in RA‐induced neuronal differentiation of P19 cells
Authors: Dai Jin‐po , Lu Jian‐yi , Zhang Ye , Shen Yu‐fei 
Published: May 2010

AbstractCovalent modifications of histone tails have fundamental roles in chromatin structure and function. Tri‐methyl modification on lysine 27 of histone H3 (H3K27me3) usually correlates with gene repression that plays important roles in cell lineage commitment and development. Mash1 is a basic helix‐loop‐helix regulatory protein that plays a critical role in neurogenesis, where it expresses as an early marker. In this study, we have shown a decreased H3K27me3 accompanying with an increased demethylase of H3K27me3 (Jmjd3) at the promoter of Mash1 can elicit a dramatically efficient expression of Mash1 in RA‐treated P19 cells. Over‐expression of Jmjd3 in P19 cells also significantly enhances the RA‐induced expression and promoter activity of Mash1. By contrast, the mRNA expression and promoter activity of Mash1 are significantly reduced, when Jmjd3 siRNA or dominant negative mutant of Jmjd3 is introduced into the P19 cells. Chromatin immunoprecipitation assays show that Jmjd3 is efficiently recruited to a proximal upstream region of Mash1 promoter that is overlapped with the specific binding site of Hes1 in RA‐induced cells. Moreover, the association between Jmjd3 and Hes1 is shown in a co‐Immunoprecipitation assay. It is thus likely that Jmjd3 is recruited to the Mash1 promoter via Hes1. Our results suggest that the demethylase activity of Jmjd3 and its mediator Hes1 for Mash1 promoter binding are both required for Jmjd3 enhanced efficient expression of Mash1 gene in the early stage of RA‐induced neuronal differentiation of P19 cells. J. Cell. Biochem. 110: 1457–1463, 2010. © 2010 Wiley‐Liss, Inc.
Bone matrix proteins and extracellular acidification: Potential co‐regulators of osteoclast morphology
Authors: Gramoun Azza , Goto Tetsuya , Nordström Tommy , Rotstein Ori D. , Grinstein Sergio , Heersche Johan N.M. , Manolson Morris F. 
Published: May 2010

AbstractOsteoclasts are signaled by the bone matrix proteins fibronectin (FN), vitronectin (VN), and osteopontin (OPN) via integrins. To perform their resorptive function, osteoclasts cycle between compact (polarized), spread (non‐resorbing) and migratory morphologies. Here we investigate the effects of matrix proteins on osteoclast morphology and how those effects are mediated using RAW 264.7 cells differentiated into osteoclasts on FN, VN, and OPN‐coated culture dishes. After 96 h, 80% of osteoclasts on FN were compact while 25% and 16% on VN were in compact and migratory states respectively. In contrast, OPN induced osteoclast spreading. Furthermore, osteoclasts formed on VN and FN were two‐ to fourfold smaller than those formed on OPN in the 21–30 nuclei/osteoclast group. These effects were not due to defects in cytoskeletal reorganization of osteoclasts on VN and FN, demonstrated by the ability of these cells to spread in response to 35 ng/ml macrophage colony stimulating factor (M‐CSF). Conversely, osteoclasts on OPN failed to spread when induced by M‐CSF. Moreover, the extracellular pH on FN and VN (7.25 and 7.3, respectively) was significantly lower than that on OPN (∼7.4). We further investigated the role of extracellular pH and found that at pH 7.5 the duration of an osteoclast's compact phase was 25.6 min and that of the spread phase was 62.5 min. Reducing the pH to 7.0 increased the frequency of osteoclast cycling by threefold. These results show that matrix proteins play a role in regulating osteoclast morphology, possibly via altering extracellular and intracellular pH. J. Cell. Biochem. 111: 350–361, 2010. © 2010 Wiley‐Liss, Inc.
OCT3/4 regulates transcription of histone deacetylase 4 (Hdac4) in mouse embryonic stem cells
Authors: Addis Russell C. , Prasad Megana K. , Yochem Robert L. , Zhan Xiangcan , Sheets Timothy P. , Axelman Joyce , Patterson Ethan S. , Shamblott Michael J. 
Published: May 2010

AbstractOCT3/4 is a POU domain transcription factor that is critical for maintenance of pluripotency and self‐renewal by embryonic stem (ES) cells and cells of the early mammalian embryo. It has been demonstrated to bind and regulate a number of genes, often in conjunction with the transcription factors SOX2 and NANOG. In an effort to further understand this regulatory network, chromatin immunoprecipitation was used to prepare a library of DNA segments specifically bound by OCT3/4 in undifferentiated mouse ES (mES) cell chromatin. One segment corresponds to a region within the first intron of the gene encoding histone deacetylase 4 (Hdac4), a Class II histone deacetylase. This region acts as a transcriptional repressor and contains at least two functional sites that are specifically bound by OCT3/4. HDAC4 is not expressed in the nuclei of OCT3/4+ mES cells and is upregulated upon differentiation. These findings demonstrate the participation of OCT3/4 in the repression of Hdac4 in ES cells. J. Cell. Biochem. 111: 391–401, 2010. © 2010 Wiley‐Liss, Inc.
Novel 14S,21‐dihydroxy‐docosahexaenoic acid rescues wound healing and associated angiogenesis impaired by acute ethanol intoxication/exposure
Authors: Tian Haibin , Lu Yan , Shah Shraddha P. , Hong Song 
Published: May 2010

AbstractAcute ethanol intoxication and exposure (AE) has been known to impair wound healing and associated angiogenesis. Here, we found that AE diminished the formation of novel reparative lipid mediator 14S,21‐dihydroxy‐docosa‐4Z,7Z,10Z,12E,16Z,19Z‐hexaenoic acid (14S,21‐diHDHA) and its biosynthetic intermediate 14S‐hydroxy‐DHA (14S‐HDHA) from docosahexaenoic acid (DHA) in murine wounds. However, AE did not reduce the formation of DHA and the intermediate 21‐HDHA. These results indicate that in the biosynthetic pathways of 14S,21‐diHDHA in wounds, AE suppresses the 14S‐hydroxy‐generating activity of 12‐lipoxygenase‐like (LOX‐like), but does not suppress the 21‐hydroxy‐generating activity of cytochrome P450 and DHA‐generating activities. The AE‐suppression of 12‐LOX‐like activity was further confirmed by the diminished formation of 12‐hydroxy‐eicosatetraenoic acid in wounds under AE. Supplementing 14S,21‐diHDHA to wounds rescued the AE‐impaired healing and vascularization. 14S,21‐diHDHA restored AE‐impaired processes of angiogenesis in vitro: endothelial cell migration, tubulogenesis, and phosphorylation of p38 mitogen‐activated protein kinase (MAPK). Taken together, the suppression of 14S,21‐diHDHA formation is responsible, at least partially, for the AE‐impairment of cutaneous wound healing and angiogenesis. Supplementing 14S,21‐diHDHA to compensate its deficit in AE‐impaired wounds rescues the healing and angiogenesis. These results provide a novel mechanistic insight for AE‐impaired wound healing that involves the necessary roles of 14S,21‐diHDHA. They also offer leads for developing 14S,21‐diHDHA‐related therapeutics to ameliorate AE‐impairment of wound healing. J. Cell. Biochem. 111: 266–273, 2010. © 2010 Wiley‐Liss, Inc.
A new nuclear protease with cathepsin L properties is present in HeLa and Caco‐2 cells
Authors: Puchi Marcia , García‐Huidobro Jenaro , Cordova Candy , Aguilar Rodrigo , Dufey Estefanie , Imschenetzky Maria , Bustos Paula , Morin Violeta 
Published: May 2010

AbstractRecently many authors have reported that cathepsin L can be found in the nucleus of mammalian cells with important functions in cell‐cycle progression. In previous research, we have demonstrated that a cysteine protease (SpH‐protease) participates in male chromatin remodeling and in cell‐cycle progression in sea urchins embryos. The gene that encodes this protease was cloned. It presents a high identity sequence with cathepsin L family. The active form associated to chromatin has a molecular weight of 60 kDa, which is higher than the active form of cathepsin L described until now, which range between 25 and 35 kDa. Another difference is that the zymogen present in sea urchin has a molecular weight of 75 and 90 kDa whereas for human procathepsin L has a molecular weight of 38–42 kDa. Based on these results and using a polyclonal antibody available in our laboratory that recognizes the active form of the 60 kDa nuclear cysteine protease of sea urchin, ortholog to human cathepsin L, we investigated the presence of this enzyme in HeLa and Caco‐2 cells. We have identified a new nuclear protease, type cathepsin L, with a molecular size of 60 kDa, whose cathepsin activity increases after a partial purification by FPLC and degrade in vitro histone H1. This protease associates to the mitotic spindle during mitosis, remains in the nuclei in binuclear cells and also translocates to the cytoplasm in non‐proliferative cells. J. Cell. Biochem. 111: 1099–1106, 2010. © 2010 Wiley‐Liss, Inc.