bluuebunny/crossref_metadata_2025_split huggingface dataset:
NCOA4 initiates ferritinophagy by binding GATE16 using two highly avid short linear interaction motifs
Authors: Lee April , Davis Joseph H. 
Published: June 2024

ABSTRACTCells carefully regulate cytosolic iron, which is a vital enzymatic cofactor, yet is toxic in excess. In mammalian cells, surplus iron is sequestered in ferritin cages that, in iron limiting conditions, are degraded through the selective autophagy pathway ferritinophagy to liberate free iron. Prior work identified the ferritinophagy receptor protein NCOA4, which links ferritin and LC3/GABARAP-family member GATE16, effectively tethering ferritin to the autophagic machinery. Here, we elucidate the molecular mechanism underlying this interaction, discovering two short linear motifs in NCOA4 that each bind GATE16 with weak affinity. These binding motifs are highly avid and, in concert, support high-affinity NCOA4•GATE16 complex formation. We further find the minimal NCOA4383-522fragment bearing these motifs is sufficient for ferritinophagy and that both motifs are necessary for this activity. This work suggests a general mechanism wherein selective autophagy receptors can distinguish between the inactive soluble pools of LC3/GABARAPs and the active membrane-conjugated forms that drive autophagy. Finally, we find that iron decreases NCOA4383-522’s affinity for GATE16, providing a plausible mechanism for iron-dependent regulation of ferritinophagy.
Metastasis-initiating osteosarcoma subpopulations establish paracrine interactions with both lung and tumor cells to create a metastatic niche
Authors: Reinecke James B. , Saraf Amanda , Hinckley John , Gross Amy C. , Pommellette Helene Le , Garcia Leyre Jimenez , Cam Maren , Cannon Matthew V. , Vatelle Sophia , Gryder Berkley E. , Dries Ruben , Roberts Ryan D. 
Published: June 2024

ABSTRACTOsteosarcoma is an aggressive and deadly bone tumor, primarily afflicting children, adolescents, and young adults. Poor outcomes for osteosarcoma patients are intricately linked with the development of lung metastasis. While lung metastasis is responsible for nearly all deaths caused by osteosarcoma, identification of biologically defined, metastasis-targeting therapies remains elusive because the underlying cellular and molecular mechanisms that govern metastatic colonization of circulating tumor cells to the lung remains poorly understood. While thousands of tumor cells are released into circulation each day, very few can colonize the lung. Herein, using a combination of a novel organotypic metastasisin vitromodel, single-cell RNA sequencing, human xenograft, and murine immunocompetent osteosarcoma models, we find that metastasis is initiated by a subpopulation of hypo-proliferative cells with the unique capacity to sustain production of metastasis promoting cytokines such as IL6 and CXCL8 in response to lung-epithelial derived IL1α. Critically, genomic and pharmacologic disruption of IL1 signaling in osteosarcoma cells significantly reduces metastatic progression. Collectively, our study supports that tumor-stromal interactions are important for metastasis, and suggests that metastatic competency is driven, in part, by the tumor cell’s ability to respond to the metastatic niche. Our findings support that disruption of tumor-stromal signaling is a promising therapeutic approach to disrupt metastasis progression.
Age-associated increased stiffness of the ovarian microenvironment impairs follicle development and oocyte quality and rapidly alters follicle gene expression
Authors: Pietroforte Sara , Plough Makenzie , Amargant Farners 
Published: June 2024

AbstractIn humans, aging triggers cellular and tissue deterioration, and the female reproductive system is the first to show signs of decline. Reproductive aging is associated with decreased ovarian reserve, decreased quality of the remaining oocytes, and decreased production of the ovarian hormones estrogen and progesterone. With aging, both mouse and human ovaries become pro-fibrotic and stiff. However, whether stiffness directly impairs ovarian function, folliculogenesis, and oocyte quality is unknown. To answer this question, we cultured mouse follicles in alginate gels that mimicked the stiffness of reproductively young and old ovaries. Follicles cultured in stiff hydrogels exhibited decreased survival and growth, decreased granulosa cell viability and estradiol synthesis, and decreased oocyte quality. We also observed a reduction in the number of granulosa cell-oocyte transzonal projections. RNA sequencing revealed early changes in the follicle transcriptome in response to stiffness. Follicles cultured in a stiff environment had lower expression of genes related to follicle development and greater expression of genes related to inflammation and extracellular matrix remodeling than follicles cultured in a soft environment. Altogether, our findings suggest that ovarian stiffness directly modulates folliculogenesis and contributes to the progressive decline in oocyte quantity and quality observed in women of advanced maternal age.
Regional dynamics in the evapotranspiration components, crop coefficients and water productivity in vineyards in the Barossa Valley
Authors: Phogat V. , Collins C. 
Published: June 2024

AbstractEstimation of water balance components, water stress and crop coefficients at different spatial scale are crucial for understanding regional dynamics in irrigation requirement and crop water use. We estimated these parameters for irrigated vineyards over 3 consecutive seasons (2018-19, 2019-20 and 2020-21) at 48 locations in the Barossa region, South Australia. We used FAO-56 dual crop coefficient approach by integrating relevant data for soil, crop, and climate parameters from the study sites. Numerous statistical error estimates, and efficiency parameters were estimated to compare and verify the predictions by FAO-56 approach. Results show a huge variability in the irrigation, water balance parameters, crop and water stress coefficients, and water productivity parameters. For instance, a coefficient of variation ranging from 20 to 97% was observed in daily and seasonal actual ET (ETcact) across different sites and seasons. Average actual transpiration (Tpact) and evaporation (Es) account for around 65 and 35% of theETcact, respectively, showing the potential to save water lost to the environment from the soil surface. Estimated actual single crop coefficient (Kcact) across all sites varied from 0.35 to 0.59, 0.16-0.62 and 0.18-0.68 during the budburst to flowering (BB-FL), flowering to veraison (FL-V), and veraison to harvest (V-H) stages of crop growth, respectively. Similarly, actual basal crop coefficients (Kcbact) for grapevine reveal immense site-specific variability questioning the adoption of uniform coefficients at subregional and regional levels. Results further demonstrate that water stress (Ks) gradually increased reaching its peak from late November to early December, with variations across the region ranging from 23 to 64%. A comparison of water productivities in relation toETcactandTpactexhibit almost 61% higher values for the latter across all the sites and subregions. Dry biomass productivity shows huge potential for renewal energy generation. Variations in the components of ET and crop coefficients are consistent with the characteristic variation in soil, topography, and microclimates. This study suggests that locally estimatedKcactandKcbactwill contribute to the efficient use of limited freshwater resources for sustainable wine grape production.
Genome-wide association analysis of flowering date in a collection of cultivated olive tree
Authors: Aqbouch Laila , Abou-Saaid Omar , Sarah Gautier , Zunino Lison , Segura Vincent , Mournet Pierre , Bonal Florelle , Zaher Hayat , Bakkali Ahmed El , Cubry Philippe , Costes Evelyne , Khadari Bouchaib 
Published: June 2024

1.AbstractFlowering date in perennial fruit trees is an important trait for fruit production. Depending on the winter and spring temperatures, flowering of olive may be advanced, delayed, or even suppressed. Deciphering the genetic control of flowering date is thus key to help selecting cultivars better adapted to the current climate context. Here, we investigated the genetic determinism of full flowering date stage in cultivated olive based on capture sequencing data of 318 genotypes from the worldwide olive germplasm bank of Marrakech, Morocco. The genetic structure of this collection was organized in three clusters that were broadly attributed to eastern, central, and western Mediterranean regions, based on the presumed origin of genotypes. Flowering dates, collected over seven years, were used to estimate the genotypic best linear unbiased predictors, which were then analyzed in a genome-wide association study. Loci with small effects were significantly associated with the studied trait, by either a single- or a multi-locus approach. The three most robust loci were located on chromosomes 01 and 04, and on a scaffold, and explained 7.1%, 6.2%, and 6.5 % of the trait variance, respectively. A significantly higher accuracy in the best linear unbiased predictors of flowering date prediction was reported with Ridge-compared to LASSO-based genomic prediction model. Along with genomic association results, this suggests a complex polygenic determinism of flowering date, as seen in many other fruit perennials. These results and the screening of associated regions for candidate genes open perspectives for further studies and breeding programs targeting flowering date.
Absolute rate coefficient measurements of the reactions of vibrationally cold
HD+
and
H3+
ions with neutral C atoms
Authors: Grussie Florian , Berger Lukas , Grieser Manfred , Kálosi Ábel , Müll Damian , Novotný Oldřich , Znotins Aigars , Dayou Fabrice , Urbain Xavier , Kreckel Holger 
Published: June 2024

Ion-neutral reactions are driving the formation of small molecules in the gas phase of interstellar clouds, where hydrogen molecules and their ions are by far the most important collision partners for any species in the astrochemical network. Here we present absolute rate coefficient measurements for the reactions HD++C→CH+/CD++D/H and H3++C→CH+/CH2++H2/H obtained using a recently commissioned ion-neutral collision setup at the Cryogenic Storage Ring. Our measurements with vibrationally cold ions result in significantly higher rate coefficients when compared with previous studies using internally excited ions, bringing them in better agreement with classical capture theories. Moreover, we have performed detailed quasiclassical trajectory (QCT) calculations for the HD++C reaction, using new potential energy surfaces. Our experimental results and the QCT calculations show very good agreement for the absolute cross section of the reactions, as well as for the isotope effect. These results have great potential relevance for the chemistry of the interstellar medium and the onset of organic chemistry in space.

Published by the American Physical Society
2024
Natural-linewidth measurements of the
3C
and
3D
soft-x-ray transitions in Ni xix
Authors: Shah Chintan , Kühn Steffen , Bernitt Sonja , Steinbrügge René , Togawa Moto , Berger Lukas , Buck Jens , Hoesch Moritz , Seltmann Jörn , Kozlov Mikhail G. , Porsev Sergey G. , Gu Ming Feng , Porter F. Scott , Pfeifer Thomas , Leutenegger Maurice A. , Cheung Charles , Safronova Marianna S. , Crespo López-Urrutia José R. 
Published: June 2024

We used the monochromatic soft-x-ray beamline P04 at the synchrotron-radiation facility PETRA III to resonantly excite the strongest 2p−3d transitions in neonlike Ni ions, [2p6]J=0→[(2p5)1/23d3/2]J=1 and [2p6]J=0→[(2p5)3/23d5/2]J=1, respectively dubbed 3C and 3D, achieving a resolving power of 15 000 and signal-to-background ratio of 30. We obtain their natural linewidths, with an accuracy of better than 10%, as well as the oscillator-strength ratio f(3C)/f(3D)=2.51(11) from analysis of the resonant fluorescence spectra. These results agree with those of previous experiments, earlier predictions, and our own advanced calculations.

Published by the American Physical Society
2024
Quantum-circuit refrigeration of a superconducting microwave resonator well below a single quantum
Authors: Viitanen Arto , Mörstedt Timm , Teixeira Wallace S. , Tiiri Maaria , Räbinä Jukka , Silveri Matti , Möttönen Mikko 
Published: June 2024

We experimentally demonstrate a recently proposed single-junction quantum-circuit refrigerator (QCR) as an tunable low-temperature environment for a superconducting 4.7 GHz resonator. With the help of a transmon qubit, we measure the populations of the different resonator Fock states, thus providing reliable access to the temperature of the engineered electromagnetic environment and its effect on the resonator. We demonstrate coherent and thermal resonator states and that the on-demand dissipation provided by the QCR can drive these to a small fraction of a photon on average, even if starting above 1 K. We observe that the QCR can be operated either with a dc bias voltage or a gigahertz rf drive, or a combination of these. The bandwidth of the rf drive is not limited by the circuit itself and consequently, we show that 2.9 GHz continuous and 10 ns pulsed drives lead to identical desired refrigeration of the resonator. These observations answer to the shortcomings of previous works where the Fock states were not resolvable and the QCR exhibited slow charging dynamics. Thus, this work introduces a versatile tool to study open quantum systems, quantum thermodynamics, and to quickly reset superconducting qubits.

Published by the American Physical Society
2024
Whether ChatGPT adoption inspires higher education students’ digital entrepreneurial intention? An integrated model of the SCCT and the TPB
Authors: Duong Cong Doanh , Nguyen Thanh Hieu , Chu Tuan Vu , Pham Thanh Van , Do Ngoc Diep 
Published: June 2024

PurposeThis study aims to integrate the Social Cognitive Career Theory with the Theory of Planned Behavior to unravel the intricate dynamics of how engaging with ChatGPT affects individuals’ digital entrepreneurial intention, underlying the serial mediation mechanism of the cognitive and reasoned career process.Design/methodology/approachThis research use a cross-sectional design, drawing on a sample of 604 higher education students from six universities in Vietnam. Multiple regression analyses were conducted to test the formulated hypotheses after assessing the reliability and validity of scales through Cronbach’s alpha and confirmatory factor analysis.FindingsThe results indicate that ChatGPT adoption significantly increases perceived AI competencies, entrepreneurial opportunity recognition and digital entrepreneurial self-efficacy. Digital entrepreneurial self-efficacy was found to have a positive impact on attitudes toward digital entrepreneurship, which, in turn, fosters digital entrepreneurial intention. ChatGPT adoption thus poses serial indirect impacts on digital entrepreneurial intention through the sequential pathways of enhanced digital entrepreneurial self-efficacy and positive attitudes.Practical implicationsThis study’s findings provide valuable recommendations for entrepreneurs, higher education institutions and policymakers.Originality/valueThis research contributes to the entrepreneurship literature by integrating two prominent theoretical frameworks to elucidate how ChatGPT adoption impacts digital entrepreneurial intentions. The serial mediation model expands that understanding of the complex cognitive and reasoned processes involved, providing a novel perspective on the role of AI in entrepreneurship.
Consumer reactions to packaging-free product dispensers: a variety of buyers' profiles to consider
Authors: Louis Didier , Durif Fabien , Lombart Cindy , Untilov Olga , Charton-Vachet Florence 
Published: June 2024

PurposeThis study investigates consumer reactions to dispensers offering solid packaging-free products (e.g. pasta, lentils, cereals) integrated in a delimitated area in a grocery store. More specifically, the research examines the impacts of the overall appeal of packaging-free solid food product dispensers and their perceived ease of use on consumers' intention to use these dispensers and purchase packaging-free products. Moreover, using a set of variables, different buyer profiles are highlighted.Design/methodology/approachThe field study for this research was conducted in a delimitated area (i.e. a shop within a shop) dedicated to solid packaging-free products (e.g. pasta, lentils, cereals) in a university cooperative store in the province of Quebec, Canada. A total of 456 buyers and consumers of packaging-free products from this store took part in the field study and completed our survey.FindingsThis study shows packaging-free dispensers' overall appeal and perceived ease of use to be determinants of consumers' intention to use these dispensers and purchase packaging-free products. The Rebus (response-based procedure for detecting unit segments) method highlights the need to consider three buyer profiles (enthusiastic, pragmatic, and sceptical) with different reactions to the specific dispensers used by retailers for packaging-free products.Originality/valueThis study focuses on buyers' reactions to packaging-free dispensers in stores, during the purchase process, whereas previous ones highlighted the drivers of and barriers to consumer adoption of packaging-free products (before the purchasing process starts). It also points to the need to fine-tune the segmentation of consumers of packaging-free products, which must be based not only on consumers' previous experience or familiarity with these products but also on the integration of their drivers and barriers.
The relationship between digital financial inclusion, gender inequality, and economic growth: dynamics from financial development
Authors: Le Quoc Dinh 
Published: June 2024

PurposeThis article employs a panel vector autoregression (PVAR) model to examine the relationship between digital financial inclusion (DFI), economic growth (EG), and gender equality (GE) across different levels of financial development.Design/methodology/approachBased on the current financial development dynamics, this study applies the PVAR method to two groups of countries: the first group represents the high financial development group, and the second group represents the low financial development group, during the period from 2015 to 2021.FindingsThe findings from impulse response functions reveal that digital financial inclusion fosters economic growth in nations with advanced financial systems, while simultaneously mitigating gender inequality. Conversely, in countries with less developed financial infrastructures, digital financial inclusion stimulates economic growth but exacerbates gender disparities. Moreover, the variance decomposition analysis indicates that the linkage between economic growth, digital financial inclusion, and gender inequality is more intertwined in countries with limited financial development than in those with well-established financial systems.Originality/valueEffective deployment of new technologies relies heavily on technological infrastructure. This policy focuses on constructing and developing information technology infrastructure to create favorable conditions for the implementation of new DFI technologies. This study also emphasizes promoting equitable education and training by ensuring that both women and men have equal opportunities to access quality education and training. This may involve investing in early childhood education, providing access to primary education, and offering scholarships to women in technology, science, and engineering fields.
Fair play in family firms: examining the perceived justice of performance management systems
Authors: Vajda Éva , Wieszt Attila , Anand Amitabh 
Published: June 2024

PurposeThis study examines the intricate relationship between family influence and perceived justice in performance management systems within family firms. Recognizing the unique dynamics that family ownership brings to human resource practices, the research aims to delineate how family presence affects both the process and the perception of fairness in performance evaluations.Design/methodology/approachUsing a conceptual framework, the research adopts a dual-method approach, combining a comprehensive literature review with theoretical modeling. The study synthesizes existing research and theoretical insights to explore the effects of family influence on the perceived fairness of performance management practices.FindingsThe findings reveal that family influence profoundly shapes fairness perceptions in performance management, impacting family and non-family employees. It affects systems' design, implementation and reception, with mechanisms including resource distribution and criteria alignment. Specifically, family influence molds fairness perceptions within the performance management process, enhancing organizational performance and fostering trust in family businesses, thus supporting sustainable growth.Originality/valueThis study contributes to the family business and human resource management literature by providing a nuanced understanding of how family dynamics influence perceptions of justice in performance management. It underscores the dual role of family influence in enhancing and complicating fairness perceptions, thus offering a balanced view that can inform academic research and practical HR management in family firms.
Prison officers’ experiences of key-working with women living in a psychologically informed planned environment (PIPE)
Authors: Till Georgia , Shah-Beckley Iduna , Harvey Joel , Kells Maisie 
Published: June 2024

Purpose
A key aspect of psychologically informed planned environments (PIPEs), are the attachment theory-informed relationships between residents and staff (Bainbridge, 2017). The key-work provision of one-to-one support from officers to residents is one of the main ways through which relationships are formed. The purpose of this paper is to explore prison officers’ experiences of the key-work role within a PIPE in a women’s Prison in England.

Design/methodology/approach
Semi-structured interviews explored ten prison officers’ experiences. Interviews were analysed using thematic analysis.

Findings
Five main themes were identified; “Professional support”, “Negotiating Professional Boundaries”, “A Successful Relationship”, “Rupture and Repair” and “Growth for Everyone”. These themes reflected the framework around keywork; what support officers need to cope with the emotional demands of the role, and how to manage challenging situations and build meaningful key-work relationships.

Research limitations/implications
Limitations include the lack of focus on diversity, the impact of the COVID-19 pandemic on officer experience and applicability to other PIPE services. Future research could address some of these limitations.

Practical implications
Practical implications highlight the need for consistent supervision, greater consideration of officers’ transition to the role and trauma-informed training.

Originality/value
The research provides an unprecedented account of prison officers’ experiences of the key-work role, adding to the limited literature within PIPEs in the women’s estate. The supportive nature of the key-work relationship was perceived by officers to contribute towards people’s sentence progression and officers’ personal and professional development.