bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microbial production of docosahexaenoic acid by a low temperature‐adaptive strain Thraustochytriidae sp. Z105: screening and optimization
Authors: Zhou Peng‐Peng , Lu Ming‐Bo , Li Wei , Yu Long‐Jiang 
Published: May 2010

AbstractAs an alternative source in addition to fish oil, microbial production of docosahexaenoic acid has been recieved more and more attentions owing to their culture advantage. A unicellular eukaryotic microbe with high DHA production and capable of low temperature‐adaptive growth was isolated from seawater and identified as Thraustochytriidae sp. Z105. The siginificant effect of temperature on cell growth and DHA synthesis by the strain was revealed. It could grow and produce DHA even at 4 °C, but hardly grow above 35 °C. Low temperature (15–25 °C) was favorable for formation of biomass, lipids and DHA, but DHA synthesis was completely blocked above 30 °C. Conditions for high level DHA production by Thraustochytriidae sp. Z105 in flask culture were optimized as follows: medium containing glucose 80 g/l, yeast extract 5.0 g/l, K2HPO4 · 3 H2O 1.0 g/l, MgSO4 · 7 H2O 0.5 g/l, seawater crystal 20 g/l, pH 6.0, liquid volume 30 ml/250 ml, temperature 20 °C, agitation speed of 200 r/min, and culture for 120 h. Under the optimal conditions, biomass of 16.72 g/l, total lipids of 5.35 g/l, DHA yield of 1.71 g/l (accounting for 32% of the total lipids) were achieved, respectively. In flask cluture level, the DHA productivity of Thraustochytriidae sp. Z105 was higher than most reported results, which suggested the wild type strain was a potential superior candidate for industrialization of DHA production. Moreover, the strain is an unique and valuable resource for investigation of the low temperature adaptive mechanism related to DHA synthesis. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
The polymorphism of protein phosphatase Z1 gene in Candida albicans
Authors: Kovács László , Farkas Ilona , Majoros László , Miskei Márton , Pócsi István , Dombrádi Viktor 
Published: May 2010

AbstractThe gene of protein phosphatase Z1 (CaPPZ1)1 that codes a fungus specific regulatory enzyme was investigated in Candida albicans. After cloning and sequencing CaPPZ1 we revealed the heterozygous nature of the ATCC 10231 reference strain, and identified two new alleles termed CaPPZ1‐2 and CaPPZ1‐3. The genetic polymorphism in CaPPZ1 was extended by finding a fourth allele CaPPZ1‐4 in a clinical isolate. Single nucleotide replacements and short in/del mutations were identified in the gene, some of which resulted in amino acid changes in the protein. The analysis of the hypervariable 3′‐noncoding gene region in 27 DNA sequences obtained from reference strains and clinical samples confirmed the presence of four distinct DNA sequence‐groups that correspond to the four main alleles of CaPPZ1. In addition to the allelic combinations, we detected individual mutations elevating genetic variability of the opportunistic pathogen. We utilized the hypervariable gene region for genotyping C. albicans in clinical isolates by sequencing the cloned amplified region, by direct sequencing of the PCR products, or by RFLP analysis. The comparison of the genotypes of the strains originating from different body parts of the same patient proved to be useful in delineating the origin of the infection. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Influence of oxidative stress and grains on sclerotial biomass and carotenoid yield of Penicillium sp. PT95
Authors: Chen Shu‐Jun , Wang Qi , Han Jian‐Rong 
Published: May 2010

AbstractOxidative stress and grains were evaluated for carotenoid production by solid‐state fermentation using Penicillium sp. PT95. When the fungus was grown at high oxidative stress, its sclerotial biomass and carotenoid content in sclerotia increased significantly with respect to low oxidative stress (P < 0.01). High oxidative stress also caused a statistically significant increase in carotenoid yield as compared with low oxidative stress (P < 0.01). Both the sclerotial biomass and the amount of carotenoid accumulated in sclerotia of strain PT95 were strongly dependent on the grain medium used. Among the grain media tested under high oxidative stress, buckwheat medium gave the highest content of carotenoid in sclerotia (828 μg/g dry sclerotia), millet medium gave respectively the highest sclerotial biomass (12.69 g/100 g grain) and carotenoid yield (10.152 mg/100 g grain). (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Identification of Mn‐related Raman modes in Mn‐doped ZnO thin films
Authors: Hu Y. M. , Wang C. Y. , Lee S. S. , Han T. C. , Chou W. Y. , Chen G. J. 
Published: May 2010

AbstractThis article aims to investigate the Raman modes present in Mn‐doped ZnO thin films that are deposited using the magnetron co‐sputtering method. A broad band ranging from 500 to 590 cm−1 is present in the Raman spectra of heavily Mn‐doped ZnO films. The multi‐peak‐fitting results show that this broad band may be composed of six peaks, and the peak at 528 cm−1 could be a characteristic mode of Mn2O3. The results of this study suggest that the origin of the Raman peaks in Mn‐doped ZnO films may be due to three major types: structural disorder and morphological changes caused by the Mn dopant, Mn‐related oxides and intrinsic host‐lattice defects. Copyright © 2010 John Wiley & Sons, Ltd.
Heterogeneity in the course of posttraumatic stress disorder: Trajectories of symptomatology
Authors: Dickstein Benjamin D. , Suvak Michael , Litz Brett T. , Adler Amy B. 
Published: May 2010

AbstractUnconditional and conditional trajectories of posttraumatic stress disorder (PTSD) symptomatology were examined using a sample of U.S. soldiers deployed on a NATO‐led peacekeeping mission to Kosovo. Data were collected at 4 time points, ranging from the weeks leading up to deployment to 9‐months postdeployment. Latent class growth analysis revealed 4 unique symptom trajectories: resilience, recovery, delayed, and unrealized anxiety. Variables identified as significant predictors of trajectory class included previous traumatic events, combat exposure, peacekeeping daily hassles, depression, alcohol use, aggressive behavior, stress reactivity, and military rank. Results from this study add to the literature detailing the variability in PTSD course, as well as to the literature pertaining to predictors of PTSD onset and course.
Parenting behaviors and posttraumatic symptoms in relation to children's symptomatology following a traumatic event
Authors: Valentino Kristin , Berkowitz Steven , Stover Carla Smith 
Published: May 2010

AbstractChild‐ and caregiver‐report about parenting behaviors, and caregiver‐report of their own symptoms were examined in relation to children's symptomatology following a potentially traumatic event (PTE) among 91 youth. Child‐report of hostile and coercive parenting was a salient predictor of child posttraumatic stress disorder (PTSD), internalizing symptoms, and personal adjustment. Caregivers' own trauma symptoms predicted caregiver‐report of child PTSD, internalizing and externalizing symptoms, but not child‐reported child symptoms. Implications for assessment and intervention following exposure to a PTE are emphasized.
Analysis of liver transplant outcomes for United Network for Organ Sharing recipients 60 years old or older identifies multiple model for end-stage liver disease-independent prognostic factors
Authors: Aloia Thomas A. , Knight Richard , Gaber Osama A. , Ghobrial Mark R. , Goss John A. 
Published: May 2010

Abstract
Older recipient age is associated with worse posttransplant survival. Although the median age of liver disease patients undergoing orthotopic liver transplantation (OLT) continues to rise, prognostic factors for posttransplant survival specific to older patients have not been defined. To address this issue, the United Network for Organ Sharing/Organ Procurement and Transplantation Network outcome database was searched to identify prognostic factors for the 8070 liver recipients 60 years old or older who underwent transplantation from 1994 to 2005. Prognostic factors were assessed with univariate analysis and multivariate modeling. The 5 strongest prognostic variables (ventilator status, diabetes mellitus, hepatitis C virus, creatinine levels ≥1.6 mg/dL, and recipient and donor age ≥120 years) were aggregated to define a novel older recipient prognostic score (ORPS). The overall 1- and 5-year posttransplant survival rates were 83% and 67%, respectively. The risk model, created by the assignment of 1 point to each ORPS factor, stratified patient outcomes into distinct prognostic groups at the 1-, 3-, and 5-year posttransplant time points (P < 0.001). The 5-year survival rates for patients with ORPS values of 0, 1, and 2 points were 75%, 69%, and 58%, respectively. Patients who underwent transplantation with an ORPS > 2 points consistently experienced 5-year survival rates of less than 50%. In conclusion, in liver transplant recipients 60 years old or older, the ORPS was able to predict significant and clinically relevant differences in posttransplant survival. By optimization of donor selection for recipients over the age of 60 years, clinical utilization of the ORPS model may enhance organ utilization for all patients awaiting OLT. Liver Transpl, 2010. © 2010 AASLD.
Factors affecting hepatocyte isolation, engraftment, and replication in an in vivo model
Authors: Kawahara Toshiyasu , Toso Christian , Douglas Donna N. , Nourbakhsh Mahra , Lewis Jamie T. , Tyrrell David Lorne , Lund Garry A. , Churchill Thomas A. , Kneteman Norman M. 
Published: May 2010

Abstract
Human hepatocyte transplantation is an alternative treatment for acute liver failure and liver diseases involving enzyme deficiencies. Although it has been successfully applied in selected recipients, both isolation and transplantation outcomes have the potential to be improved by better donor selection. This study assessed the impact of various donor variables on isolation outcomes (yield and viability) and posttransplant engraftment, using the SCID/Alb-uPA (severe combined immunodeficient/urokinase type plasminogen activator under the control of an albumin promoter) human liver chimeric mouse model. Human hepatocytes were obtained from 90 human liver donor specimens and were transplanted into 3942 mice. Multivariate analysis revealed improved viability with younger donors (P = 0.038) as well as with shorter warm ischemic time (P = 0.012). Hepatocyte engraftment, assessed by the posttransplant level of serum human α1-antitrypsin, was improved with shorter warm ischemia time. Hepatocytes isolated from older donors (≥60 years) had lower viability and posttransplant engraftment (P ≤ 0.01). In conclusion, the selection of young donors (<60 years) and rapid liver specimen retrieval, allowing for shorter warm ischemia time, are key determinants for the success of both the isolation of high viability human hepatocytes and their subsequent posttransplantation capacity for engraftment and expansion. Liver Transpl 16:974-982, 2010. © 2010 AASLD.
Brand‐consumer storytelling theory and research: Introduction to a Psychology & Marketing special issue
Authors: Woodside Arch G. 
Published: May 2010

AbstractThis introduction reviews core principles of storytelling theory. The article explains basic propositions of good storytelling. A brief summary of each of the six articles that follow appears. The article extends a note of appreciation to the members of the special editorial board for this issue and to Rajan Nataraajan. © 2010 Wiley Periodicals, Inc.
Movement disorders associated with moyamoya disease: A report of 4 new cases and a review of literatures
Authors: Baik Jong Sam , Lee Myung Sik 
Published: May 2010

AbstractThe aim of this study was to define the clinical characteristics of patients who developed movement disorders in association with moyamoya disease (MMD). Using PubMed and medical records of our hospital from 1985 to 2008, we searched for patients who developed movement disorders in association with MMD. This study included 38 patients described in previous studies and 4 patients found in the medical records. The onset of movement disorders was thought to be sudden. In 13 patients, the movement disorders were precipitated by hyperventilation or emotional stress. Twenty‐seven of the 42 patients developed chorea, 4 patients developed dystonia, and 4 developed a mixture of both. The movement disorders of the remaining 7 patients were described as dyskinesia. A third of the 42 patients developed bilateral movement disorders, and their mean age was younger than that of those with unilateral movement disorders. In 37 of the 42 patients, brain imaging studies showed ischemic lesions, but the remaining 5 patients showed no parenchymal lesions. Cerebral perfusion studies showed hypoperfusion in the basal ganglia and in the cerebral cortical areas. Most patients improved whether they were treated or not. MMD must be included in the differential diagnosis of the sudden onset of dyskinesias, particularly chorea and focal dystonia. Even in patients with no parenchymal lesion in brain imaging studies, cerebral angiography and cerebral blood perfusion studies must be performed, if they develop a sudden onset or recurrent movement disorders preceded by emotional stress or hyperventilation. © 2010 Movement Disorder Society
Speech disturbances in patients with dystonia or chorea due to neurometabolic disorders
Authors: Flamand‐Rouvière Constance , Guettard Emilie , Moreau Caroline , Bahi‐Buisson Nadia , Valayannopoulos Vassili , Grabli David , Motte Jacques , Rodriguez Diana , Roubertie Agathe , Maintigneux Lisa , Kemlin Isabelle , Ceballos‐Picot Irène , Adams David , Vidailhet Marie , Roze Emmanuel 
Published: May 2010

AbstractSpeech disturbances are frequent and potentially disabling in patients with dystonia or chorea due to neurometabolic disorders (DCND), but their precise characteristics are poorly documented. We prospectively studied 29 consecutive patients with DCND. A detailed description of their speech patterns was obtained by using the Frenchay dysarthria assessment test and the apraxia of speech evaluation test of Wertz. Gross motor function and intelligibility were each scored on 5‐point scales to identify a possible correlation between the severity of the speech and motor disorders. All the patients were found to have complex speech alterations with combined features of hyperkinetic dysarthria and speech apraxia. We also noted a correlation between the severity of the speech disorders and the motor disorders. These findings have important implications for speech rehabilitation, and may provide new insights into the pathophysiology of dystonia due to neurometabolic disorders. © 2010 Movement Disorder Society
Parkinson's disease symptoms: The patient's perspective
Authors: Politis Marios , Wu Kit , Molloy Sophie , G. Bain Peter , Chaudhuri K. Ray , Piccini Paola 
Published: May 2010

AbstractPatients suffering from Parkinson's disease (PD) will typically experience a range of motor and nonmotor symptoms during the course of their illness, each of which will affect a particular individual to varying degrees. However, patients' perceptions of troublesome symptoms often differ from the clinician's view, and these discrepancies can hamper effective management of PD. In this study, we have assessed 265 consecutive PD patients by asking them to rank their three most troublesome symptoms in the last 6 months, so to gain further insight from the impact of illness on patients' quality of life. Patients were divided into early (<6 years) and late PD groups (≥6 years) from symptom onset. The division at 6 years was based on the mean time from symptom onset to the development of motor complications. In the early PD group, the 5 most prevalent complaints (ranked in descending order) are slowness, tremor, stiffness, pain, and loss of smell and/or taste. In the advanced PD group, fluctuating response to their medication (most common: wearing‐off phenomenon followed by dyskinesia), mood changes, drooling, sleep problems (most common: middle and late night insomnia followed by daytime sleepiness), and tremor were the top 5. Our findings provide further evidence for the diversity of experience in PD and suggest that as the disease advances the most troublesome issues that patients perceive are the lack of response to medication and the nonmotor aspects of the disease, highlighting the importance of assessment and patient‐centered management in the follow‐up of these patients. © 2010 Movement Disorder Society
Application of Random Forest and Multiple Linear Regression Techniques to QSPR Prediction of an Aqueous Solubility for Military Compounds
Authors: Kovdienko Nikolay A. , Polishchuk Pavel G. , Muratov Eugene N. , Artemenko Anatoly G. , Kuz'min Victor E. , Gorb Leonid , Hill Frances , Leszczynski Jerzy 
Published: May 2010

AbstractThe relationship between the aqueous solubility of more than two thousand eight hundred organic compounds and their structures was investigated using a QSPR approach based on Simplex Representation of Molecular Structure (SiRMS). The dataset consists of 2537 diverse organic compounds. Multiple Linear Regression (MLR) and Random Forest (RF) methods were used for statistical modeling at the 2D level of representation of molecular structure. Statistical characteristics of the best models are quite good (MLR method: R2=0.85, Q2=0.83; RF method: R2=0.99, R2oob=0.88). The external validation set of 301 compounds (including 47 nitro‐, nitroso‐ and nitrogen‐rich compounds of military interest) which were not included in the training set and modeling process, was used for evaluation of the models predictivity. Thus, well‐fitted and robust (R2test(MLR)=0.76 and R2test(RF)=0.82) models were obtained for both statistical techniques using descriptors based on the topological structural information only. The predicted solubility values for military compounds are in good agreement with experimental ones. Developed QSPR models represent powerful and easy‐to‐use virtual screening tool that can be recommended for prediction of aqueous solubility.
Multiplex RARE: A simultaneous multislice spin‐echo sequence that fulfils CPMG conditions
Authors: Lee Kuan J. , Zahneisen Benjamin , Hennig Jürgen , Weigel Matthias , Leupold Jochen 
Published: May 2010

AbstractThis work presents a new imaging sequence in which multiple slices are simultaneously excited and refocused in a spin‐echo train. The multiple spin‐echo trains are interleaved in such a manner that (i) the Carr‐Purcell‐Meiboom‐Gill conditions are fulfilled at all times, and (ii) the signals from slices can be separated, preventing aliasing. This paper also demonstrates how the sequence may be used in a novel fat‐water Dixon method that enables fast volume coverage. The technique is demonstrated in phantoms and in vivo. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.
Data convolution and combination operation (COCOA) for motion ghost artifacts reduction
Authors: Huang Feng , Lin Wei , Börnert Peter , Li Yu , Reykowski Arne 
Published: May 2010

AbstractA novel method, data convolution and combination operation, is introduced for the reduction of ghost artifacts due to motion or flow during data acquisition. Since neighboring k‐space data points from different coil elements have strong correlations, a new “synthetic” k‐space with dispersed motion artifacts can be generated through convolution for each coil. The corresponding convolution kernel can be self‐calibrated using the acquired k‐space data. The synthetic and the acquired data sets can be checked for consistency to identify k‐space areas that are motion corrupted. Subsequently, these two data sets can be combined appropriately to produce a k‐space data set showing a reduced level of motion induced error. If the acquired k‐space contains isolated error, the error can be completely eliminated through data convolution and combination operation. If the acquired k‐space data contain widespread errors, the application of the convolution also significantly reduces the overall error. Results with simulated and in vivo data demonstrate that this self‐calibrated method robustly reduces ghost artifacts due to swallowing, breathing, or blood flow, with a minimum impact on the image signal‐to‐noise ratio. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.
Contrast agent influences MRI phase‐contrast flow measurements in small vessels
Authors: Lagerstrand Kerstin M. , Vikhoff‐Baaz Barbro , Starck Göran , Forssell‐Aronsson Eva 
Published: May 2010

AbstractContrast‐enhanced MR angiography is often combined with phase contrast (PC) flow measurement to answer a particular clinical question. The contrast agent that is administered during contrast‐enhanced MR angiography may still be present in the blood during the consecutive PC flow measurement. The aim of this work was to evaluate the influence of contrast agent on PC flow measurements in small vessels. For that purpose, both in vivo measurements and computer simulations were performed. The dependence of the PC flow quantification on the signal amplitude difference between blood and stationary background tissue for various vessel sizes was characterized. Results show that the partial‐volume effect strongly affects the accuracy of the PC flow quantification when the imaged vessel is small compared to the spatial resolution. A higher blood‐to‐background‐contrast level during imaging significantly increases the partial‐volume effect and thereby reduces the accuracy of the flow quantification. On the other hand, a higher blood‐to‐background‐contrast level facilitated the segmentation of the vessel for flow rate determination. PC flow measurements should therefore be performed after contrast agent administration in large vessels, but before contrast agent administration in small vessels. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.
Accelerated 3D catheter visualization from triplanar MR projection images
Authors: Schirra Carsten Oliver , Weiss Steffen , Krueger Sascha , Caulfield Denis , Pedersen Steen F. , Razavi Reza , Kozerke Sebastian , Schaeffter Tobias 
Published: May 2010

AbstractOne major obstacle for MR‐guided catheterizations is long acquisition times associated with visualizing interventional devices. Therefore, most techniques presented hitherto rely on single‐plane imaging to visualize the catheter. Recently, accelerated three‐dimensional (3D) imaging based on compressed sensing has been proposed to reduce acquisition times. However, frame rates with this technique remain low, and the 3D reconstruction problem yields a considerable computational load. In X‐ray angiography, it is well understood that the shape of interventional devices can be derived in 3D space from a limited number of projection images. In this work, this fact is exploited to develop a method for 3D visualization of active catheters from multiplanar two‐dimensional (2D) projection MR images. This is favorable to 3D MRI as the overall number of acquired profiles, and consequently the acquisition time, is reduced. To further reduce measurement times, compressed sensing is employed. Furthermore, a novel single‐channel catheter design is presented that combines a solenoidal tip coil in series with a single‐loop antenna, enabling simultaneous tip tracking and shape visualization. The tracked tip and catheter properties provide constraints for compressed sensing reconstruction and subsequent 2D/3D curve fitting. The feasibility of the method is demonstrated in phantoms and in an in vivo pig experiment. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.