bluuebunny/crossref_metadata_2025_split huggingface dataset:
Preparation, Characterization, and Assessment of Antimicrobial Properties of Silver-doped Hydroxyapatite Nanoparticles in Orthodontic Composite
Authors: Rajan KV Ranjeth , Nagesh Shweta , SP Mani 
Published: May 2024

Aim: The incorporation of nanoparticles in orthodontic materials like composites and primers can be used to combat white spot lesions (WSLs), which is a common complication of orthodontic treatment. Silver-doped hydroxyapatite nanoparticles (AgHANps) have the potential to produce antibacterial effects against caries-causing bacteria in the oral cavity and can be applied for combating WSL when incorporated in orthodontic composites. Hence, the primary objective of this study is to characterize and assess the antibacterial properties of orthodontic composites containing AgHANps. Materials and Methods: The structure of the synthesized AgHANps was studied using scanning electron microscopy (SEM), energy-dispersive X-ray analysis (EDAX), and Fourier transform infrared spectroscopy (FTIR). Commercially available orthodontic composite was incorporated with the prepared AgHANps. The agar-well diffusion method was used to investigate the AgHANps-incorporated composite’s antibacterial properties against Staphylococcus aureus ( S. aureus), Streptococcus mutans ( S. mutans), and Escherichia coli ( E. coli). ANOVA and Bonferroni post-hoc test was used to assess the differences in zones of inhibition (ZOI) between the groups with the p value set at .05. Results: The AgHANPs was prepared and characterized using EDAX and FTIR, which revealed the successful formation of the nanoparticles without any contaminants. After the integration of the AgHANps into the orthodontic composite, antimicrobial assessment showed statistically significant ZOI against S. aureus ( p = .003), S. mutans (0.001), and E. coli (0.003). The high concentration of the AgHANPS (40 μL) showed the largest ZOI against all the bacteria followed the control group and the low concentration (20 μL) group. Conclusion: The study shows the AgHANps incorporated orthodontic adhesive possess antimicrobial effect against S. aureus, S. mutans, and E. coli and the effect increases with the concentration of the nanoparticle.
Association Between Knee Flexor Strength and Preservation of the Tibial Attachment of the Sartorial Fascia During Hamstring Tendon Harvest
Authors: Catma Mehmet Faruk , Dulgeroglu Deniz , Guroz Ali Nazmican , Kuzucu Yakup 
Published: May 2024

Background: During anterior cruciate ligament (ACL) reconstruction, there are various autograft options. Donor-site morbidity is an important consideration while deciding the type of the autograft. Gracilis and semitendinosus autografts are commonly used in ACL reconstruction, resulting in weakness of the hamstring muscle. Hypothesis: We hypothesized that if we preserved the tibial insertion site of the sartorial fascia (SF) during hamstring tendon harvest, there would be better recovery of knee flexor strength. Study Design: Case-control study; Level of evidence, 3. Methods: In this retrospective study, 34 patients (aged 20-59 years) underwent ACL reconstruction using hamstring tendon autograft with 2 different incision techniques on the SF. The tibial attachment site of the SF was preserved in 17 patients. The insertion site of the muscle was incised transversely in 17 patients. The follow-up duration was ≥2 years. Patients were recalled to the institute for examination and muscle strength assessment. The results were compared between the groups in terms of flexor and extensor knee isokinetic muscle strength at 60 and 180 deg/s. Results: There was no statistical difference between the groups in terms of age, sex, or body mass index. When compared with patients whose SF attachment site was incised, patients with a preserved SF tibial insertion were found to have a higher flexion peak torque at the angular speed of 180 deg/s ( P < 002). No statistically significant difference was noted at 60 deg/s. Conclusion: During collection of gracilis and semitendinosus autografts, preserving the SF tibial attachment site was associated with better knee flexion peak torque.
Voices of unpaid carers: problems and prospects in accessing palliative care and self-care information, resources and services
Authors: Van Dinther Kristine , Javanparast Sara 
Published: May 2024

Background: Unpaid carers make a substantial contribution to the health economy and carers of palliative patients are particularly vulnerable due to special patient needs and excessive carer burden. The Australian Government recently implemented the Integrated Carer Support Service Model to provide a range of free services to carers in the community. However, it is unclear whether such initiatives are effective and, more importantly, how carers of palliative patients gain access to information, support and services for the patient and themselves. Objectives: We sought to investigate unpaid carers’ experiences in accessing information and resources for support with patient care with a specific focus on palliative care resources and to determine carers’ access to information and support for self-care. We also aimed to identify what opportunities and challenges remain for these particular carers according to their experiences. Methods: We conducted 18 semi-structured interviews and 3 focus groups with unpaid family or friend carers of palliative patients in South Australia from metropolitan, regional and rural communities. Grounded in a descriptive phenomenological paradigm, we conducted a hybrid approach to thematic analysis combining deductive and inductive coding following Fereday and Muir-Cochrane’s method. Results: The government’s web-based initiative provided little impact in supporting carers from our cohort. There remains a substantial gap between the formal recognition of the importance of carers and their lived reality. This recognition by health professionals is vital, as carer self-identification is not common and affects help-seeking behaviour. Carers seek and respond to more grassroots, personalized forms of support and sharing of information. Conclusion: The lack of self-identification affects carers’ help-seeking behaviours. Carer identification and recognition need to be initiated by health professionals in a proactive manner to ensure carers are prepared for their role and are emotionally supported to sustain it. Carers seek face-to-face guidance and sources of information.
Health Planning in Times of COVID-19 in Burkina Faso: The Role of Its National Strategic Pandemic Management Committee
Authors: Druetz Thomas , Bicaba Frank , Zainabou Cissé , Bicaba Abel 
Published: May 2024

Context Presenting the COVID-19 crisis as a pandemic misleadingly implies a certain homogeneity between the regions of the Globe in terms of their burden and reactions. However, from the outset of the crisis, countries presented different epidemiological realities and sometimes adopted divergent, even opposing measures. Curiously, the heterogeneity of responses persisted as scientific evidence accumulated about COVID-19 and the strategies for dealing with it. Case study This commentary aims to recount the specific experience of Burkina Faso, and how it reoriented its initial biomedical response into a multisectoral strategy. Burkina Faso set up a committee specifically to examine the effects not only of the pandemic, but also of the control measures. This committee was mandated to decompartmentalize the lens through which the COVID-19 was dealt with. It entered into dialogue with a level of stakeholders often overlooked during national health crisis: communities. As a member of this “National Committee for Crisis Management of the Pandemic”, one of the co-authors contributed to its orientations and has witnessed first-hand some of the challenges it faced. Recommendations This experience suggests that the project of extricating the field of public health from medicine is advancing in Burkina Faso. In order to manage future crises more effectively and across different sectors, there is an urgent need to establish state structures and to strengthen public health systems. States need coordination units that have the legitimacy, authority and resources required to mobilize a variety of actors at the community, national and international levels.
Proactive inhibitory control as a cognitive contributor to social difficulties in adolescents with ADHD traits
Authors: McKay Erin , Kirk Hannah , Cornish Kim 
Published: May 2024

Adolescents with attention deficit hyperactivity disorder (ADHD) represent a significantly heterogenous group in terms of cognitive and social ability. Given the high co-occurrence of ADHD and autism, it is unclear whether the social difficulties seen in ADHD may be attributed to the same underlying factors which contribute to social difficulties in autism, or are a unique feature of ADHD. This study explores differences in cognition in adolescents with clinically elevated ADHD symptoms with and without social difficulties, and adolescents with ADHD symptoms and autism. Thirty-nine adolescents completed objective measures of cognition (attention, working memory, inhibition) and social cognition (affect recognition, theory of mind [ToM]). Adolescents with ADHD symptoms and social difficulties had poorer proactive inhibition than both those without social difficulties, and those with autism. Lower cognitive performance was only significantly related to social functioning for adolescents with ADHD who experienced social difficulties. ToM was weakest in those with autism. Social difficulties in adolescents with clinically elevated ADHD symptoms are associated with poor proactive inhibition, working memory and affect recognition. This highlights the potential benefit of cognitive strategies for remediating social difficulties in ADHD, and the need for ADHD specific interventions. Lay abstract Around half of teenagers with attention deficit hyperactivity disorder (ADHD) experience significant difficulties forming and maintaining friendships. This in turn often has negative effects on schooling and mental health. There is a large body of research that suggests the social difficulties commonly seen in ADHD may be due to weaknesses in skills such as impulse control, working memory, recognising emotions, and perspective taking. Given teenagers with autism often present with similar weaknesses and social difficulties, it is not known whether the social difficulties experienced by teenagers with ADHD have the same underlying causes. If so, this may suggest that social difficulties in ADHD represent undiagnosed autism, or low-level autism traits. This study compared these key skills in teenagers with clinical ADHD symptoms who experience social difficulties, with those who don’t experience social difficulties, and those with co-occurring autism. The results showed that social difficulties in ADHD and autism appear to have different underlying causes, suggesting that the social difficulties seen in teenagers with ADHD are unique to this disorder, and are not due to underlying autism, or autism traits. This highlights the need for social skills interventions, particularly those that target impulse control, emotion recognition and working memory, that are specifically tailored to the needs and weaknesses of teenagers with ADHD, rather than using existing interventions designed for teenagers with autism.
Integrated insulin-iron nanoparticles: a multi-modal approach for receptor-specific bioimaging, reactive oxygen species scavenging, and wound healing
Authors: Attri Komal , Chudasama Bhupendra , Mahajan Roop L. , Choudhury Diptiman 
Published: May 2024

AbstractMetallic nanoparticles have emerged as a promising option for various biological applications, owing to their distinct characteristics such as small size, optical properties, and ability to exhibit luminescence. In this study, we have successfully employed a one-pot method to synthesize multifunctional insulin-protected iron [Fe(II)] nanoparticles denoted as [IFe(II)NPs]. The formation of IFe(II)NPs is confirmed by the presence of FTIR bonds at 447.47 and 798.28 cm−1, corresponding to Fe–O and Fe–N bonds, respectively. Detailed analysis of the HR-TEM-EDS-SAED data reveals that the particles are spherical in shape, partially amorphous in nature, and have a diameter of 28.6 ± 5.2 nm. Additionally, Metal Ion Binding (MIB) and Protein Data Bank (PDB) analyses affirm the binding of iron ions to the insulin hexamer. Our findings underscore the potential of IFe(II)NPs as a promising new platform for a variety of biomedical applications due to their high signal-to-noise ratio, and minimal background fluorescence. The particles are highly luminescent, biocompatible, and have a significant quantum yield (0.632). Exemplar applications covered in this paper include insulin receptor recognition and protection against reactive oxygen species (ROS), harmful molecules known to inflict damage on cells and DNA. The IFe(II)NPs effectively mitigate ROS-induced inflammation, which is a hinderance to wound recovery, thereby facilitating enhanced wound recovery.
Graphical abstract
Fluocinolone acetonide 0.2 µg/day intravitreal implant in non-infectious uveitis affecting the posterior segment: EU expert user panel consensus-based clinical recommendations
Authors: Pleyer Uwe , Pavesio Carlos , Miserocchi Elisabetta , Heinz Carsten , Devonport Helen , Llorenç Víctor , Burke Tomás , Nogueira Vanda , Kodjikian Laurent , Bodaghi Bahram 
Published: May 2024

Abstract
Background
Non-infectious uveitis affecting the posterior segment of the eye (NIU-PS) is an inflammatory disease, which can significantly impair visual acuity if not adequately treated. Fluocinolone-acetonide sustained-release-0.2 µg/day intravitreal (FAc) implants are indicated for prevention of relapse in recurrent NIU-PS. The aim here was to provide treating clinicians with some consensus-based-recommendations for the clinical management of patients with NIU-PS with 0.2 µg/day FAc implants.

Methods
A European-clinical-expert-group agreed to develop a consensus report on different issues related to the use of FAc implants in patients with NIU-PS.

Results
The Clinical-expert-panel provided specific recommendations focusing on clinical presentation (unilateral/bilateral) of the NIU-PS; systemic involvement of NIU-PS and the lens status. Treatment algorithms were developed; one that refers to the management of patients with NIU-PS in clinical practice and another that establishes the best clinical scenarios for the use of FAc implants, both as monotherapy and as adjuvant therapy. Additionally, the Clinical-expert-panel has provided recommendations about the use of the FAc implants in a clinical-setting. The Clinical-expert-panel also considered the safety profile of FAc implants and their possible implications in the daily practice.

Conclusions
As more clinical experience has been gained using FAc implants, it was necessary to update the clinical recommendations that guide patient management in the clinic. The current consensus document addresses relevant issues related to the use of FAc implants on different types of patients with various etiologies of NIU-PS, and was conducted to standardize approaches to help specialists obtain better clinical outcomes.
From bench to bedside: US-Japan Collaborative Workshop on the NVU
Authors:   2023 US-Japan NVU Workshop Group, Borlongan Cesario , Esposito Elga , Corales Laarni Grace , Hattori Yorito , Hayashi Yu , Ihara Masafumi , Iliff Jeffrey J. , Kisler Kassandra , Kitazume Shinobu , Koizumi Schuichi , Liu Jialing , Maki Takakuni , Onodera Osamu , Saito Satoshi , Sawamoto Kazunobu , Sohya Kazuhiro , Taguchi Akihiko , Takahashi Shinichi , Tanaka Kenji , Taoka Toshiaki , Wake Hiroaki , Yuzaki Michisuke 
Published: May 2024

AbstractThe joint workshop between U.S. and Japanese researchers, supported by The U.S.–Japan Brain Research Cooperative Program, convened in January 2023 at Keio University Mita campus in Tokyo, Japan. The workshop had a threefold objective. Firstly, it aimed to facilitate robust exchanges between U.S. and Japanese researchers engaged in Neurovascular Unit (NVU) research, enhancing the global network of scholars in the field. Secondly, it aimed to encourage the initiation of collaborative research projects, fostering interdisciplinary efforts and synergistic advancements in understanding the brain vascular physiology and central nervous system. Lastly, the workshop emphasized the nurturing of young researchers, recognizing their pivotal role in shaping the future of NVU research. Throughout the workshop, participants discussed fundamental aspects of the NVU, exploring its complex connections and vital functions. By sharing their expertise and insights, the workshop attendees sought to uncover novel approaches to mitigate the burden of neurological diseases for individuals worldwide. This report provides a summary of the presentations and discussions held during the workshop, showcasing the collective efforts and progress made by the participants.
A guided network estimation approach using multi-omic information
Authors: Bartzis Georgios , Peeters Carel F. W. , Ligterink Wilco , Van Eeuwijk Fred A. 
Published: May 2024

Abstract
Intoduction
In systems biology, an organism is viewed as a system of interconnected molecular entities. To understand the functioning of organisms it is essential to integrate information about the variations in the concentrations of those molecular entities. This information can be structured as a set of networks with interconnections and with some hierarchical relations between them. Few methods exist for the reconstruction of integrative networks.

Objective
In this work, we propose an integrative network reconstruction method in which the network organization for a particular type of omics data is guided by the network structure of a related type of omics data upstream in the omic cascade. The structure of these guiding data can be either already known or be estimated from the guiding data themselves.

Methods
The method consists of three steps. First a network structure for the guiding data should be provided. Next, responses in the target set are regressed on the full set of predictors in the guiding data with a Lasso penalty to reduce the number of predictors and an L2 penalty on the differences between coefficients for predictors that share edges in the network for the guiding data. Finally, a network is reconstructed on the fitted target responses as functions of the predictors in the guiding data. This way we condition the target network on the network of the guiding data.

Conclusions
We illustrate our approach on two examples in Arabidopsis. The method detects groups of metabolites that have a similar genetic or transcriptomic basis.
Adaptive divergence, historical population dynamics, and simulation of suitable distributions for Picea Meyeri and P. Mongolica at the whole-genome level
Authors: Liu Yifu , Xiao Wenfa , Wang Fude , Wang Ya , Dong Yao , Nie Wen , Tan Cancan , An Sanping , Chang Ermei , Jiang Zeping , Wang Junhui , Jia Zirui 
Published: May 2024

AbstractThe taxonomic classification of Picea meyeri and P. mongolica has long been controversial. To investigate the genetic relatedness, evolutionary history, and population history dynamics of these species, genotyping-by-sequencing (GBS) technology was utilized to acquire whole-genome single nucleotide polymorphism (SNP) markers, which were subsequently used to assess population structure, population dynamics, and adaptive differentiation. Phylogenetic and population structural analyses at the genomic level indicated that although the ancestor of P. mongolica was a hybrid of P. meyeri and P. koraiensis, P. mongolica is an independent Picea species. Additionally, P. mongolica is more closely related to P. meyeri than to P. koraiensis, which is consistent with its geographic distribution. There were up to eight instances of interspecific and intraspecific gene flow between P. meyeri and P. mongolica. The P. meyeri and P. mongolica effective population sizes generally decreased, and Maxent modeling revealed that from the Last Glacial Maximum (LGM) to the present, their habitat areas decreased initially and then increased. However, under future climate scenarios, the habitat areas of both species were projected to decrease, especially under high-emission scenarios, which would place P. mongolica at risk of extinction and in urgent need of protection. Local adaptation has promoted differentiation between P. meyeri and P. mongolica. Genotype‒environment association analysis revealed 96,543 SNPs associated with environmental factors, mainly related to plant adaptations to moisture and temperature. Selective sweeps revealed that the selected genes among P. meyeri, P. mongolica and P. koraiensis are primarily associated in vascular plants with flowering, fruit development, and stress resistance. This research enhances our understanding of Picea species classification and provides a basis for future genetic improvement and species conservation efforts.
Comprehensive identification, characterization, and expression analysis of the MORF gene family in Brassica napus
Authors: Xing Jiani , Zhang Yayi , Song Wenjian , Ali Nadia Ahmed , Su Kexing , Sun Xingxing , Sun Yujia , Jiang Yizhou , Zhao Xiaobo 
Published: May 2024

Abstract
Background
RNA editing in chloroplast and mitochondrion transcripts of plants is an important type of post-transcriptional RNA modification in which members of the multiple organellar RNA editing factor gene family (MORF) play a crucial role. However, a systematic identification and characterization of MORF members in Brassica napus is still lacking.

Results
In this study, a total of 43 MORF genes were identified from the genome of the Brassica napus cultivar “Zhongshuang 11”. The Brassica napus MORF (BnMORF) family members were divided into three groups through phylogenetic analysis. BnMORF genes distributed on 14 chromosomes and expanded due to segmental duplication and whole genome duplication repetitions. The majority of BnMORF proteins were predicted to be localized to mitochondria and chloroplasts. The promoter cis-regulatory element analysis, spatial-temporal expression profiling, and co-expression network of BnMORF genes indicated the involvement of BnMORF genes in stress and phytohormone responses, as well as growth and development.

Conclusion
This study provides a comprehensive analysis of BnMORF genes and lays a foundation for further exploring their physiological functions in Brassica napus.
Transcriptome-based identification and validation of reference genes for corm growth stages, different tissues, and drought stress in Taro (Colocasia esculenta)
Authors: Dong Weiqing , Chen Qi , He Fanglian 
Published: May 2024

AbstractTaro is a widely utilized starch resource plant. It is essential to quantify the expression levels of functional genes associated with taro growth using real-time quantitative polymerase chain reaction (RT-qPCR). However, to obtain reliable RT-qPCR results, appropriate reference genes (RGs) are required for data normalization. In this study, we screened seven novel candidate RGs using transcriptome datasets from taro, encompassing data from growth corms and various tissues. The expression stability of these seven new RGs, along with the commonly used RGs Actin, EF1-α, and β-tubulin, was assessed using Delta Ct, BestKeeper, geNorm, and NormFinder algorithms. Furthermore, we conducted a comprehensive analysis using the RefFinder program and validated the results using the target gene, CeAGPL1. The findings revealed that ACY-1 and PIA2 were the optimal multiple RGs for normalization during corm growth, while COX10 and Armc8 were suitable for samples including various types of tissues. Furthermore, we found three RGs, Armc8, COX10 and CCX4L, were the optimal RGs for drought stress. This study assessed the suitability of RGs in taro for the first time. The identified RGs provide valuable resources for studying corm growth, diverse tissues, and drought stress. This study contributes to the advancement of our understanding of the underlying mechanisms that govern the growth of taro.