bluuebunny/crossref_metadata_2025_split huggingface dataset:
Differential expression of FSTL1 and its correlation with the pathological process of periodontitis
Authors: Jiang Wenxin , Yu Weijun , Hu Shucheng , Shi Yuanjie , Lin Lu , Yang Ruhan , Tang Jiaqi , Gu Yuting , Gong Yuhua , Jin Min , Lu Eryi 
Published: May 2024

AbstractAimsThis study aimed to elucidate the alterations in Follistatin‐like protein 1 (FSTL1) and its association with the pathological process of periodontitis.MethodsThis study included 48 patients with periodontitis and 42 healthy controls. The expression level of FSTL1 in the gingiva was determined by RT‐qPCR, validated using the dataset GSE16134, and subsequently examined by western blotting. Bioinformatics analysis revealed a single‐cell distribution of FSTL1, characteristic of angiogenesis and immune cell infiltration. The expression and distribution of FSTL1, vascular endothelial marker protein CD31 and myeloperoxidase (MPO), the indicator of neutrophil activity, were determined by immunohistochemistry (IHC). A series of correlation analyses was performed to determine the associations between FSTL1 and clinical parameters, including probing depth (PD) and clinical attachment loss (CAL), and their potential role in angiogenesis (CD31) and neutrophil infiltration (MPO).ResultsFSTL1 was significantly upregulated in the gingiva of patients with periodontitis compared to their healthy counterparts. In addition, FSTL1 was positively correlated with the clinical parameters PD (r = .5971, p = .0005) and CAL (r = .6078, p = .0004). Bioinformatic analysis and IHC indicated that high FSTL1 expression was significantly correlated with angiogenesis and neutrophil infiltration in periodontitis. Moreover, receiver operating characteristic (ROC) analysis demonstrated that FSTL1 could serve as an independent indicator for evaluating the severity of periodontitis (area under the curve [AUC] = 0.9011, p < .0001).ConclusionThis study demonstrated FSTL1 upregulation in periodontitis and its potential contribution to the disease via angiogenesis and neutrophil infiltration.
Differences in maternal subgingival microbiome between preterm and term births: The MOHEPI study
Authors: Park Jung Soo , Kim Eunha , Kwon Sun Jae , Heo Ju Sun , Ahn Ki Hoon 
Published: May 2024

AbstractAimPeriodontitis is a potential risk factor for preterm birth (PTB) in women; however, the causal relationship or the exact mechanism remain unknown. This study aimed to compare the oral microbiome features of mothers with full‐term birth (FTB) with those who had preterm delivery.MethodsThis study prospectively enrolled 60 women (30 mothers with PTB and 30 mothers with FTB), and subgingival plaque samples were collected and analysed by metagenomic 16S rDNA sequencing. Clinical measurements, including periodontal probing depth, clinical attachment level, modified gingival index (mGI) and plaque index, were performed to determine the periodontal state of the participants. Medical and obstetric data were collected as well.ResultsAmong the periodontal measurements, mGI score, reflecting the level of gingival inflammation, exhibited a statistically significant association with PTB (adjusted odds ratio 2.705, 95% confidence interval 1.074–6.811, p = .035). When subgroup analysis was conducted based on mean mGI scores (mGI ≥ 2, high inflammation [HI] versus mGI < 2, low inflammation [LI]), microbiome analysis revealed clear distinctions in microbial compositions between PTB and FTB mothers in both the HI and LI groups. Especially in the HI group, alpha diversity exhibited a decreasing trend in PTB mothers compared to FTB mothers. Beta diversity also revealed significant differences between the two groups. In Linear Discriminant Analysis Effect Size analysis, certain anaerobic taxa, including the genera Spirochaetes, Treponema and Porphyromonas, were relatively abundant in the FTB/HI group, whereas the PTB/HI group showed a high abundance of the order Actinomycetales. Network analysis showed that the FTB/HI had relatively stronger connectivity in microbial composition than the PTB/HI group. Dysbiosis ratio of plaque microbiome, in terms of periodontitis, was significantly lower in PTB/HI group compared to FTB/HI group.ConclusionThe compositions of maternal subgingival microbiomes differed between PTB and FTB mothers in both the high and low levels of gingival inflammation groups. In the presence of high level of gingival inflammation, dysbiosis in plaque microbiome, in terms of periodontitis, was decreased in PTB mothers compared to FTB mothers.
Counter‐gradient variation in gene expression between fish populations facilitates colonization of low‐dissolved oxygen environments
Authors: Fox Janay A. , Hunt David A. G. A. , Hendry Andrew P. , Chapman Lauren J. , Barrett Rowan D. H. 
Published: May 2024

AbstractThe role of phenotypic plasticity during colonization remains unclear due to the shifting importance of plasticity across timescales. In the early stages of colonization, plasticity can facilitate persistence in a novel environment; but over evolutionary time, processes such as genetic assimilation may reduce variation in plastic traits such that species with a longer evolutionary history in an environment can show lower levels of plasticity than recent invaders. Therefore, comparing species in the early stages of colonization to long‐established species provides a powerful approach for uncovering the role of phenotypic plasticity during different stages of colonization. We compared gene expression between low‐dissolved oxygen (DO) and high‐DO populations of two cyprinid fish: Enteromius apleurogramma, a species that has undergone a recent range expansion, and E. neumayeri, a long‐established native species in the same region. We sampled tissue either immediately after capture from the field or after a 2‐week acclimation under high‐DO conditions, allowing us to test for both evolved and plastic differences in low‐DO vs high‐DO populations of each species. We found that most genes showing candidate‐evolved differences in gene expression did not overlap with those showing plastic differences in gene expression. However, in the genes that did overlap, there was counter‐gradient variation such that plastic and evolved gene expression responses were in opposite directions in both species. Additionally, E. apleurogramma had higher levels of plasticity and evolved divergence in gene expression between field populations. We suggest that the higher level of plasticity and counter‐gradient variation may have allowed rapid genetic adaptation in E. apleurogramma and facilitated colonization. This study shows how counter‐gradient variation may impact the colonization of divergent oxygen environments.
The F‐box protein OsFBX156 positively regulates rice defence against the blast fungus Magnaporthe oryzae by mediating ubiquitination‐dependent degradation of OsHSP71.1
Authors: Zhao Yudan , Zhong Xionghui , Xu Guojuan , Zhu Xiaoying , Shi Yanlong , Liu Minghao , Wang Ruyi , Kang Houxiang , You Xiaoman , Ning Yuese , Wang Guo‐Liang , Wang Xuli 
Published: May 2024

AbstractF‐box protein is a subunit of the SCF (SKP1‐CUL1‐F‐box protein) E3 ubiquitin ligase complex, which plays a critical role in regulating different pathways in plant immunity. In this study, we identified the rice (Oryza sativa) F‐box protein OsFBX156, which targets the heat shock protein 70 (OsHSP71.1) to regulate resistance to the rice blast fungus Magnaporthe oryzae. Overexpression of OsFBX156 or knockout of OsHSP71.1 in rice resulted in the elevation of pathogenesis‐related (PR) genes and an induction burst of reactive oxygen species (ROS) after flg22 and chitin treatments, thereby enhancing resistance to M. oryzae. Furthermore, OsFBX156 can promote the degradation of OsHSP71.1 through the 26S proteasome pathway. This study sheds lights on a novel mechanism wherein the F‐box protein OsFBX156 targets OsHSP71.1 for degradation to promote ROS production and PR gene expression, thereby positively regulating rice innate immunity.
A user‐friendly method to get automated pollen analysis from environmental samples
Authors: Gimenez Betty , Joannin Sébastien , Pasquet Jérôme , Beaufort Luc , Gally Yves , de Garidel‐Thoron Thibault , Combourieu‐Nebout Nathalie , Bouby Laurent , Canal Sandrine , Ivorra Sarah , Limier Bertrand , Terral Jean‐Frédéric , Devaux Céline , Peyron Odile 
Published: May 2024

Summary

Automated pollen analysis is not yet efficient on environmental samples containing many pollen taxa and debris, which are typical in most pollen‐based studies. Contrary to classification, detection remains overlooked although it is the first step from which errors can propagate. Here, we investigated a simple but efficient method to automate pollen detection for environmental samples, optimizing workload and performance.

We applied the YOLOv5 algorithm on samples containing debris and c. 40 Mediterranean plant taxa, designed and tested several strategies for annotation, and analyzed variation in detection errors.

About 5% of pollen grains were left undetected, while 5% of debris were falsely detected as pollen. Undetected pollen was mainly in poor‐quality images, or of rare and irregular morphology. Pollen detection remained effective when applied to samples never seen by the algorithm, and was not improved by spending time to provide taxonomic details. Pollen detection of a single model taxon reduced annotation workload, but was only efficient for morphologically differentiated taxa.

We offer guidelines to plant scientists to analyze automatically any pollen sample, providing sound criteria to apply for detection while using common and user‐friendly tools. Our method contributes to enhance the efficiency and replicability of pollen‐based studies.
Ultraviolet protection in eye and face protection against biological hazards
Authors: Dain Stephen J. , Cheng Brian B. , Roy Maitreyee 
Published: May 2024

AbstractBackgroundIn the development of eye and face protection standards against biological hazards, it is important to consider that the eye and face protectors may be used in the outdoor environment, where ultraviolet (UV) exposure from the sun is much higher than indoors. Workers affected include paramedics, ambulance officers and clinical testing personnel.MethodsSpectral transmittances (280–780 nm) were measured on eight face shields and one goggle of the types used in hospital and clinical environments, three overhead projector sheets and an occupational impact protective goggle. The UV and luminous transmittances were calculated according to the international and two national sunglass standards, and compared with the compliance requirements.ResultsAll the face shields and goggle lenses were made of variants of the same material. All the samples, including the overhead projector sheets, complied with the requirements of the sunglass standards (for normal conditions, in the case of the American standard).ConclusionSince all the lenses complied with the sunglass standards for UV protection, and there appears to be uniformity in the choice of materials in this product type, there is no need to make provision, as is the case for occupational eye and face protection standards, for protectors that do not provide UV protection. This makes labelling and advice to end users from eyecare and safety professionals much simpler.
Early ray‐finned herbivores: the dental system of Eurynotoidiidae (Actinopterygii; middle–late Permian, European Russia) and implications for palaeobiology and palaeoecology
Authors: Bakaev Aleksandr S. , Bulanov Valery V. , Kogan Ilja , Johanson Zerina , Minikh Alla V. 
Published: May 2024

AbstractEurynotoidiformes are a little‐known group of actinopterygian fishes from the Permian of European Russia, characterized by the possession of multicuspid marginal teeth arranged in a single row. Morphologically, the teeth resemble those of Recent Cichlidae, Acanthuridae, Siganidae or Serrasalmidae, suggesting similar trophic adaptations related to herbivory. Tooth histology is similar to the majority of basal actinopterygians (composed of dentine, acrodin and collar enamel). Teeth are ankylosed in their attachment, and labial pleurodont in implantation, strengthening the tooth. The multicuspid tooth crowns derive from conical teeth of predatory or omnivorous ancestors, analogous to the evolution of multicuspid teeth in cichlid fishes. Tooth replacement in eurynotoidiforms is most comparable to an alternating pattern, with a possible simultaneous, unilateral replacement occurring in the whole jaw, similar to characiform fishes. Replacement teeth were formed extraosseously. Teeth of the inner dental arcade were conical. Based on comparisons with the teeth of extant actinopterygians specialized for herbivory, along with functional morphological analysis and consideration of wear patterns, we hypothesize that eurynotoidiforms represent the oldest known actinopterygians specialized for herbivory. Several strategies of herbivory in Recent actinopterygians were already realized by eurynotoidiforms as early as the Late Palaeozoic (middle and late Permian): grazing/cutting filamentous algae (Lapkosubia spp., Isadia suchonensis), browsing/biting off macrophyte fragments (Isadia aristoviensis), scraping/harvesting periphyton from hard substrates (Isadia opokiensis, I. arefievi). However, notable morphological differences in the jaws (elongate) and tooth arrangement (homodont along the jaw, functional teeth separated) suggest that this Permian experiment in herbivory followed different pathways compared to extant taxa.
Procedural sedation and analgesia in pediatric diagnostic and interventional radiology: An expert DELPHI consensus document developed by the ITALIAN scientific society of anesthesia, analgesia, resuscitation and intensive care (SIAARTI)
Authors: Mondardini Maria Cristina , Pezzato Stefano , Meneghini Luisa , Agostiniani Rino , De Cassai Alessandro , D'Errico Ignazio , Minardi Carmelo , Sagredini Raffaella , Sbaraglia Fabio , Testoni Caterina , Toni Francesco , Vason Milo , Amigoni Angela 
Published: May 2024

AbstractBackgroundChildren undergoing diagnostic and interventional radiology procedures often require sedation to achieve immobility and analgesia if the procedure is painful. In the past decades, leading scientific organizations have developed evidence‐based guidelines for procedural sedation and analgesia in children outside of the operating room. Their recommendations are being applied to procedural sedation in radiology. However, some questions remain open regarding specific aspects contextualized to the radiology setting, such as elective prone sedation, the urgency of the procedure, when venous access or airway protection is required, and others.AimsTo address the unresolved issues of procedural sedation and analgesia in pediatric diagnostic and interventional radiology.MethodsAn expert panel of pediatricians, pediatric anesthesiologists, intensivists, and neuroradiologists selected topics representative of current controversies and formulated research questions. Statements were developed by reviewing the literature for new evidence, comparing expertise and experience, and expressing opinions. Panelists' agreement with the statements was collected anonymously using the DELPHI method.ResultsTwelve evidence‐based or expert opinion incorporate are presented, considering risks, benefits, and applicability.ConclusionsThis consensus document, developed by a multidisciplinary panel of experts involved in the field, provides statements to improve the quality of decision‐making practice in procedural sedation and analgesia in pediatric radiology.
A pioneer nematode effector suppresses plant reactive oxygen species burst by interacting with the class III peroxidase
Authors: Rui Lin , Wen Tong‐Yue , Qiu Yi‐Jun , Yang Dan , Ye Jian‐Ren , Wu Xiao‐Qin 
Published: May 2024

AbstractBursaphelenchus xylophilus is the pathogen of pine wilt disease, which can devastate the pine forest ecosystem. Usually, plant cells generate reactive oxygen species (ROS) as a defensive substance or signalling molecules to resist the infection of nematodes. However, little is known about how B. xylophilus effectors mediate the plant ROS metabolism. Here, we identified a pioneer B. xylophilus Prx3‐interacting effector 1 (BxPIE1) expressed in the dorsal gland cells and the intestine. Silencing of the BxPIE1 gene resulted in reduced nematode reproduction and a delay in disease progression during parasitic stages, with the upregulation of pathogenesis‐related (PR) genes PtPR‐3 (class Ⅳ chitinase) and PtPR‐9 (peroxidase). The protein–protein interaction assays further demonstrated that BxPIE1 interacts with a Pinus thunbergii class III peroxidase (PtPrx3), which produces H2O2 under biotic stress. The expression of BxPIE1 and PtPrx3 was upregulated during the infection stage. Furthermore, BxPIE1 effectively inhibited H2O2 generating from class III peroxidase and ascorbate can recover the virulence of siBxPIE1‐treated B. xylophilus by scavenging H2O2. Taken together, BxPIE1 is an important virulence factor, revealing a novel mechanism utilized by nematodes to suppress plant immunity.
Diverse geotropic responses in the orchid family
Authors: Chen Jhun‐Chen , Lin Hsiang‐Yin , Novák Ondřej , Strnad Miroslav , Lee Yung‐I. , Fang Su‐Chiung 
Published: May 2024

AbstractIn epiphytes, aerial roots are important to combat water‐deficient, nutrient‐poor, and high‐irradiance microhabitats. However, whether aerial roots can respond to gravity and whether auxin plays a role in regulating aerial root development remain open‐ended questions. Here, we investigated the gravitropic response of the epiphytic orchid Phalaenopsis aphrodite. Our data showed that aerial roots of P. aphrodite failed to respond to gravity, and this was correlated with a lack of starch granules/statolith sedimentation in the roots and the absence of the auxin efflux carrier PIN2 gene. Using an established auxin reporter, we discovered that auxin maximum was absent in the quiescent center of aerial roots of P. aphrodite. Also, gravity failed to trigger auxin redistribution in the root caps. Hence, loss of gravity sensing and gravity‐dependent auxin redistribution may be the genetic factors contributing to aerial root development. Moreover, the architectural and functional innovations that achieve fast gravitropism in the flowering plants appear to be lost in both terrestrial and epiphytic orchids, but are present in the early diverged orchid subfamilies. Taken together, our findings provide physiological and molecular evidence to support the notion that epiphytic orchids lack gravitropism and suggest diverse geotropic responses in the orchid family.
A history of strategies and a tapestry of triumphant tales in tackling plant fungal diseases
Authors: Bailey Sarah L. , Morier‐Gxoyiya Césarée , Puthanvila Surendrababu Swathy , Saunders Diane G. O. 
Published: May 2024

AbstractEver since the first crops were domesticated over 10,000 years ago, pests and pathogens have wreaked havoc on crop production. Despite diverse threats from longstanding and emerging diseases, plants continue to play a central role in meeting humanity's requirements for calories and nutrients. In this review, we discuss historical advances made by plant pathologists and breeders that have helped keep many plant diseases at bay. We focus predominantly on fungal diseases of five food crops—rice, wheat, maize, soybean and potato—that provide the bulk of calories consumed globally. We cover such historical triumphs in plant pathology as (i) advanced knowledge of fungal biology revolutionizing disease management strategies, (ii) integration of genetics by the ‘wheat wizard’ Rowland Biffen and others transforming the landscape of resistance breeding and (iii) the advent of modern fungicides—from a sinking ship in the 1660s—leading to dramatic yield enhancements. We also discuss (iv) the potential for emerging biocontrol agents to holistically promote plant health and yield. Today, the high homogeneity of crops in modern agricultural systems exacerbates yield losses due to disease by increasing pest/pathogen transmission rates and eroding natural spatial barriers to pest/pathogen dispersal. Meanwhile, climate change is altering the compositions of global pest and pathogen populations and expanding pest/pathogen territories with unpredictable consequences. As we look to the future, the strength of the plant pathology community remains central to developing innovative solutions to tackle these complex and expanding threats to plant health and secure a plentiful food supply.
A place for people's knowledge in climate evidence: Exploring civic evidence in climate litigation
Authors: Berti Suman Anna , Burnette Amelia 
Published: May 2024

AbstractThis article examines the possibilities for data gathered by individuals and communities to demonstrate climate impacts on people's lives in domestic and international climate litigation, as well as the likely procedural constraints that such evidence may encounter. Building on recent decisions of domestic, regional and international courts and bodies, and looking in particular at cases related to climate displacement, we consider the potential for civic evidence to provide valuable testimony in climate litigation, for example, grounding abstract and diffuse harms in personal and locally relevant frames. The article concludes by advancing a research agenda to test, and support or disprove, the argument developed that civic evidence from climate‐affected people could be more robustly deployed in climate litigation and could have a complementary and reinforcing, rather than competing, role alongside institutional evidence.
Private Wealth Over the Life Cycle: A Meeting Between Microsimulation and Structural Approaches
Authors: Galiana Lino , Wilner Lionel 
Published: May 2024

AbstractThis paper embeds a structural model of private wealth accumulation over the life cycle within a dynamic microsimulation model designed for long‐run projections of pensions. In such an environment, the optimal savings path results from consumption smoothing and bequests motives, on top of the mortality risk. Preferences are estimated based on a longitudinal wealth survey through a method of simulated moments. Simulations issued from these estimations replicate quite well a private wealth that is more concentrated than labor income. They enable us to compute “augmented” standards of living including capital income, hence to quantify both the countervailing role played by private wealth to earnings dropout after retirement and the impact of the mortality risk in this regard.