bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pemberian Serbuk Daun Kelor (Moringa oleifera) terhadap Perbaikan Profil Lipid dan Ketebalan Aorta pada Tikus Model Prediabetes Mellitus
Authors: Ariestiningsih Ayuningtyas Dian , Cempaka Anggun Rindang , Kusumastuty Inggita , Cahyaningrum Aladhiana , Setiawati Sindi , Arintya Diandra , Azmi Ulul , Salwa Putri , Malahayati Huzaifah , Wibowo Rizka Azhari , Handayani Dian 
Published: June 2024

Latar Belakang: Konsumsi makanan dan minuman kemasan dengan kandungan tinggi fruktosa mengalami peningkatan beberapa tahun terakhir. Hal ini meningkatkan risiko terjadinya sindrom metabolik, meliputi resistensi insulin, peningkatan tekanan darah, dan gangguan profil lipid. Daun kelor (Moringa oleifera) dengan kandungan senyawa bioaktif yang berfungsi sebagai antioksidan diketahui mampu memperbaiki metabolisme lemak dan glukosa darah.
Tujuan: Penelitian ini bertujuan untuk mengetahui pengaruh serbuk daun kelor (Moringa oleifera) dalam mengontrol kondisi sindrom metabolik melalui identifikasi profil lipid dan ketebalan aorta pada prediabetes mellitus.
Metode: Penelitian ini merupakan studi eksperimental dengan pendekatan post test only control group design. Sebanyak 35 ekor tikus putih (Rattus novergicus) galur Wistar jantan dibagi menjadi 5 kelompok yaitu kelompok K0 (pakan standar), E1 (sonde fruktosa 66% selama 16 minggu), E2 (sonde fruktosa 66% selama 12 minggu), E3 (sonde fruktosa 66%+kuersetin 50 mg/kg BB), dan E4 (sonde fruktosa 66%+serbuk daun kelor 500 mg/kg BB). Data dianalisis menggunakan uji One Way ANOVA.
Hasil penelitian: Kadar kolesterol total, HDL kolesterol, dan ketebalan dinding aorta berbeda signifikan antarkelompok perlakuan. Tidak terdapat perbedaan yang signifikan pada kadar trigliserida, LDL kolesterol, dan massa jaringan lemak putih antar kelompok perlakuan.
Kesimpulan: Pemberian serbuk daun kelor selama 4 minggu menunjukkan perbaikan profil lipid dan ketebalan dinding aorta pada kondisi prediabetes mellitus.
Tinjauan Literatur: Hubungan Obesitas dan Infertilitas pada Pria Usia Produktif
Authors: Putri Allisa Nadhira Permata Arinda , Nadhiroh Siti Rahayu 
Published: June 2024

Latar Belakang: Obesitas merupakan masalah epidemi dunia yang mengalami kenaikan secara terus menerus. Obesitas berpengaruh negatif pada beberapa fungsi dalam tubuh yaitu termasuk fungsi reproduksi (fertilitas). Prevalensi pasangan infertil di Indonesia pada tahun 2013 sebesar 15-25% dengan infertilitas pada pria menyumbang 25-30% dari total kasus infertilitas.
Tujuan: Penelitian ini bertujuan untuk mengetahui hubungan antara obesitas dan infertilitas pada pria usia produktif.
Metode: Studi penelitian ini menggunakan metode literature review bersumber dari hasil penelitian berdasarkan kriteria inklusi berupa literatur berkredibel baik dengan topik obesitas dan infertilitas pria sebanyak 8 penelitian observasional. Referensi yang tidak dapat diakses secara full text dan penelitian pada hewan uji coba termasuk dalam kriteria ekskusi penelitian.
Ulasan: Obesitas dapat mempengaruhi kesuburan pria secara langsung atau tidak langsung melalui beberapa mekanisme perubahan profil hormonal. Obesitas dapat menyebabkan adanya perubahan androgen menjadi estrogen secara berlebihan sehingga dapat meningkatkan aktivitas aromatase dan reproduksi selanjutnya sehingga ketidakseimbangan ini dapat menurunkan kualitas dari sperma. Pada keadaan obesitas sendiri menyebabkan kadar leptin plasma yang tinggi. Peningkatan stres oksidatif yang diinduksi leptin dan produksi reactive oxygen species (ROS) yang tidak terkendali sehingga berdampak negatif pada fluiditas membran plasma sperma, merusak motilitas sperma, dan meningkatkan kerusakan DNA (deoxyribonuclealic acid) sperma.
Kesimpulan: Peningkatan body mass index (BMI) meningkatkan risiko kejadian penurunan parameter sperma yang mengacu pada peningkatan derajat infertilitas pada pria. Pria obesitas dapat meningkatkan risiko perubahan parameter sperma secara kuantitatif (volume, jumlah, dan konsetrasi) serta kualitatif (motilitas, morfologi, dan DNA sperma).
Legal and Moral Principles as Guidelines for Carrying Out Official Duties
Authors: Saputro Aries , Radian Salman  
Published: June 2024

Law and morals are related as an ideal value in the formation of law, because morals are considered universal values ​​that should ideally characterize every legal formation, with the hope that morality in the law can produce good behavior for legal subjects and objects. These morals and laws are in our administrative law, in this case the executive. The problem that is difficult to solve is the problem of corruption, where the act is legally and morally not good or wrong. Anti-corruption regulations are in place and the establishment of the Corruption Eradication Commission reveals that the problem of corruption in our country is not resolved and is even more systematic. Ideally, with the advancement of civilization and the strengthening of moral values ​​as the basis for rules, corruption will decrease to its lowest point from year to year as a government develops. Then, if this law represents a moral value, then the court decision, which is the law, can also contain moral values. As an example also regarding court decisions by state administration officials that are not implemented voluntarily, this can be equated with blatant disobedience to the law making state administration officials arrogant. Normative juridical analysis used in research includes legal and regulatory theory. Therefore, in order to achieve conformity regarding the morals and behavior of state officials in carrying out their duties, there is a close connection between the bad morals of state officials and behavior that violates the law. The good morals of state officials will make the behavior of state officials high and far from breaking the law.
Experimental Study of the Swelling and Rheological Properties of a High-Strength Preformed Particle Gel Lost Circulation Material
Authors: Zhu Yuecheng , Bai Yingrui , Sun Jinsheng , Lv Kaihe 
Published: June 2024

Summary
Preformed particulate gel (PPG) has emerged as a widely utilized lost circulation material in deep oil and gas drilling operations. The objective of our study was to devise a high-strength preformed particle gel (HSPPG) specifically designed to address drilling fluid loss in high-temperature fractured formations. To achieve this, a comprehensive set of laboratory experiments was conducted to assess the swelling and rheological properties of HSPPG under various conditions, and these investigations aimed to provide deeper insights into the pressure-bearing mechanism exhibited by HSPPG. The synthesis of HSPPG involved the copolymerization of acrylamide (AM) and N-hydroxymethacrylamide (NMA) molecular chains, catalyzed by organic peroxides, to form the primary network. Additionally, to enhance its temperature resistance, urea-formaldehyde (UF) resin, known for its superior thermal stability, was incorporated into the secondary network. This unique combination of primary and secondary networks imparted remarkable thermal endurance and structural stability to the resulting HSPPG. The swelling and rheological experiments revealed that HSPPG, with a particle size of 1000 µm, exhibited an equilibrium swelling rate (SR) value of 30.55 and a storage modulus (G’) of 1050 Pa at 120℃. These findings attested to its excellent temperature resistance and structural stability. Furthermore, when subjected to a sodium chloride solution at a temperature of 120℃ and a concentration of 25.0%, HSPPG achieved equilibrium swelling with an SR value of 24.93 and a G’ of approximately 7000 Pa. This significant increase in structural strength was attributed to charge shielding within the highly concentrated brine environment. In the plugging experiments, a wedge-shaped slit with an inlet of 3 mm and an outlet of 1 mm was successfully blocked using a concentration of 4% of HSPPG with a particle size of 1000 μm. The blocking strength achieved was 8.06 MPa. The results of these experiments, as well as the observed filling and plugging state of HSPPG in steel fractured cores, indicated that HSPPG possesses the properties of water absorption, swelling, and extrusion filling. These attributes facilitate the effective formation of a dense blocking layer within the fracture space, exhibiting excellent pressure-bearing capacity. In conclusion, the HSPPG developed in this study represents an advanced swellable granular plugging agent with excellent swelling capacity and structural strength at high temperatures. It offers an ideal solution to mitigate drilling fluid loss from fractured formations under high-temperature and high-salinity conditions.
Potential of Heterorhabditis bacteriophora JAAN-19 for sustainable management for prickly pear cactus weevil Metamasisus (Syn. Cactophagus) spinolae
Authors: Chávez-Díaz Ismael Fernando , Sáenz-Aponte Adriana , Pérez-Ricardo Vicente , Cortez-Madrigal Hipolito , Andrade-Hoyos Petra , Hernández-Dominguez Carmela , Aragón-Magadan Marco Aurelio , Grifaldo-Alcántara Pedro Fabián 
Published: June 2024

Abstract

The prickly pear cactus, is a crop of cultural, gastronomic, and economic significance in Mexico due to its diverse uses. One of the major challenges to its yield is the cactus weevil. While agrochemicals have been the primary method of control, various alternatives have been explored for sustainable agriculture using traps, parasitoids and entomopathogenic fungi with differential mortality between male and female pests, with some reaching a maximum effectiveness of 70-80%. This study explored the potential of the nematode identified as Heterorabditis bacteriophora JAAN-19 as a biocontrol agent. In vitro, a concentration of 1000 Jl/weevil achieved 90% mortality in females and males after six days. Notably, the substrate experiments, mimicking real-world conditions, demonstrated the nematode's strong searching and infective capacity, reaching 97% mortality at the same concentration within 6-8 days. Experiments revealed that the nematode's effectiveness depended on dose and exposure time, not the weevils' sex. Based on the experiment observations, the lethal dose (LD50) for the weevils was 138 Jl/weevil, and the dose for 90% mortality (LD90) was 463 Jl/weevil, both within a 6-8 day period. These findings support the high agrobiotechnological potential of H. bacteriophora JAAN-19 as a sustainable control strategy against the cactus weevil Metamasius spinolae.
TrkB-dependent antidepressants action involves Dlx5/6 inhibition in cortical GABAergic neurons.
Authors: Narboux-Nême Nicolas , Aouci Rym , Fontaine Anastasia , Vion Amin , Levi Giovanni , Belz Lou 
Published: June 2024

Abstract

Major depressive disorder (MDD) is a complex and devastating illness that affects people of all ages. However, both the aetiology of MDD and the mechanisms of action of antidepressants are not completely understood. Many indications suggest the involvement of Parvalbumin-positive GABAergic neurons (PV-neurons) in the pathogenesis of MDD. DLX5 and DLX6 (DLX5/6) encode for two homeodomain transcription factors involved in cortical GABAergic differentiation and function. In the mouse, the level of expression of these genes is inversely correlated to the cortical density of PV-neurons and to anxiety-like behaviours. The same genomic region generates the lncRNA DLX6-AS1 which, in humans, has been identified as the most central hub gene in the interneuron module downregulated in schizophrenia and ASD. PV-neurons are complex inhibitory interneurons affected in many neuro-psychiatric conditions including MDD and schizophrenia. Here, we show that the expression levels of Dlx5/6 in the adult mouse brain are inversely correlated with immobility time in the forced swimming test, an assay used to study of depressive-like behaviours and the efficacy of anti-depressive drugs in rodents. We show that the administration of the antidepressant Fluoxetine (Flx) to normal mice induces, within 24h, a rapid and stable reduction of Dlx5, Dlx6 and Dlx6-AS1 expression in the cerebral cortex through the activation of the TrkB-CREB cascade and can counteract the behavioural and cellular alterations induced by experimental Dlx5 overexpression. Our findings show that one of the short-term effects of Flx treatment is the reduction of Dlx5/6 expression in GABAergic neurons, which, in turn has direct consequences on PV expression and on behavioural profiles. Variants in the DLX5/6 regulatory network could be implicated in the predisposition to depression and in the variability of patients’ response to treatment and can constitute a target for further understanding the mechanism of action of antidepressants.
Association between Antenatal Corticosteroids and Neonatal Outcomes among Very Preterm Infants Born to Mothers with Hypertensive Disorders of Pregnancy: A Multicenter Cohort Study
Authors: Sun Mengya , Qian Aimin , Li Xianghong , Bai Ruimiao , Cheng Ping , Gu Xinyue , Wang Yanchen , Cao Yun , Zhou Wenhao , Lee Shoo K. , Jiang Hong , Jiang Siyuan 
Published: June 2024

Abstract

Background
The relationship between antenatal corticosteroids (ACS) and preterm infants born to mothers with hypertensive disorders of pregnancy (HDP) remains a subject of debate. To evaluate whether the use of ACS before delivery was associated with neonatal outcomes in very preterm infants born to mothers with HDP.
Methods
This multicenter cohort study enrolled all infants with gestational age at 24 to 31 week and admitted to tertiary NICUs of the Chinese Neonatal Network (CHNN) within 24 hours of birth from 2019 to 2021. ACS administration was defined as at least one dose of dexamethasone or betamethasone before delivery. The primary outcome was surfactant and/ or invasive mechanical ventilation (IMV) within 72 hours of life. Multivariable logistic regression analyses were performed to assess the association between ACS and neonatal outcomes.
Results
Among the 4,582 study infants born to mothers with HDP, 3,806 (83.1%) were exposed to ACS. ACS treatment was significantly associated with lower risk of requirement of surfactant and/ or IMV within 72 hours of life (adjusted Odds Ratio = 0.60, 95% confidence interval 0.49–0.74). ACS exposure was also independently associated with decreased mortality, surfactant use, IMV, combined surfactant and IMV use and moderate or severe bronchopulmonary dysplasia. The severity of maternal HDP did not appear to influence the correlation between ACS treatment and neonatal outcomes. Our analysis also indicated that a single complete course seemed to have the most significant protective effect.
Conclusions
Our study reinforces the significant role of ACS in reducing severe respiratory morbidity and mortality in very preterm infants born to mothers with HDP.
Finite-element analysis of different fixation types after Enneking II+III pelvic tumor resection
Authors: Sun Yu , Xue Haowen , Wang Xiaonan , Zhang Jiaxin , Xu Zezhou , Guo Yunting , Xin Renlong , Yu Zhenglei , Han Qing , Zhao Xin , Wang Jincheng , Luquan Ren 
Published: June 2024

Abstract

The current primary treatment approach for malignant pelvic tumors involves hemipelvic prosthesis reconstruction following tumor resection. In cases of Enneking type II + III pelvic tumors, the prosthesis necessitates fixation to the remaining iliac bone. Prevailing methods for prosthesis fixation include the saddle prosthesis, ice cream prosthesis, modular hemipelvic prosthesis, and personalized prosthetics using three-dimensional printing. To prevent failure of hemipelvic arthroplasty protheses, a novel fixation method was designed and finite element analysis was conducted. In clinical cases, the third and fourth sacral screws broke, a phenomenon also observed in the results of finite element analysis. Based on the original surgical model, designs were created for auxiliary dorsal iliac, auxiliary iliac bottom, auxiliary sacral screw, and auxiliary pubic ramus fixation. A nonlinear quasi-static finite element analysis was then performed under the maximum load of the gait cycle, and the results indicated that assisted sacral dorsal fixation significantly reduces stress on the sacral screws and relative micromotion exceeding 28 µm. The fixation of the pubic ramus further increased the initial stability of the prosthesis and its interface osseointegration ability. Therefore, for hemipelvic prostheses, incorporating pubic ramus support and iliac back fixation is advisable, as it provides new options for the application of hemipelvic tumor prostheses.
Psilocin fosters neuroplasticity in iPSC-derived human cortical neurons
Authors: Schmidt Malin , Hoffrichter Anne , Davoudi Mahnaz , Horschitz Sandra , Lau Thorsten , Meinhardt Marcus , Spanagel Rainer , Ladewig Julia , Köhr Georg , Koch Philipp 
Published: June 2024

Abstract

Psilocybin is studied as innovative medication in anxiety, substance abuse and treatment-resistant depression. Animal studies show that psychedelics promote neuronal plasticity by strengthening synaptic responses and protein synthesis. However, the exact molecular and cellular changes induced by psilocybin in the human brain are not known. Here, we treated human cortical neurons derived from induced pluripotent stem cells with the 5-HT2A receptor agonist psilocin - the psychoactive metabolite of psilocybin. We analyzed how exposure to psilocin affects 5-HT2A receptor localization, gene expression, neuronal morphology, synaptic markers and neuronal function. Upon exposure of human neurons to psilocin, we observed a decrease of cell surface-located 5-HT2A receptors first in the axonal- followed by the somatodendritic-compartment. Psilocin further provoked a 5-HT2A-R-mediated augmentation of BDNF abundance. Transcriptomic profiling identified gene expression signatures priming neurons to neuroplasticity. On a morphological level, psilocin induced enhanced neuronal complexity and increased expression of synaptic proteins, in particular in the postsynaptic-compartment. Consistently, we observed an increased excitability and enhanced synaptic network activity in neurons treated with psilocin. In conclusion, exposure of human neurons to psilocin might induces a state of enhanced neuronal plasticity which could explain why psilocin is beneficial in the treatment of neuropsychiatric disorders where synaptic dysfunctions are discussed.
Perspectives, Preparedness and Challenges of the Sudden Shifting of Online to Offline Learning in Higher Education in Bangladesh
Authors: Mojumder Bithi , Uddin Md. Jasim , Dey Kamol 
Published: June 2024

Abstract

While numerous studies have examined the pros and cons of online teaching and learning during the pandemic, and others have delved into the psychological consequences among university students, there is a gap in the literature regarding students' perspectives on adapting to changing learning environments as educational institutions navigate the post-pandemic landscape. This research investigates the intricate dynamics of tertiary-level students' experiences during the transition from online to offline learning in the post-pandemic era in Bangladesh. A survey methodology, incorporating both closed and open-ended questions, was employed to capture the diverse perspectives, adaptive strategies, and sentiments of 294 students across multiple universities in Bangladesh to gauge the sentiments of students regarding this new educational landscape. The results unveiled a nuanced perspective, reflecting both the challenges and opportunities presented by digital learning platforms. Despite the convenience of online learning, only a minority of respondents, comprising 14%, found it to be more motivating compared to traditional offline methods. A staggering 75% emphasized the importance of face-to-face contact, highlighting the irreplaceable value of in-person interaction in the educational experience. Financial barriers emerged as a significant hurdle, with 41% of participants citing cost as the primary impediment to internet access. Additionally, 51% identified signal availability and strength issues as key challenges, underscoring the digital divide exacerbated by uneven access to technology and connectivity. However, amidst these challenges, a beacon of hope emerged in the form of technological integration. An overwhelming majority of students, constituting 79%, expressed a desire for instructors to leverage technology in offline classes to enhance engagement and capture students' attention. Nevertheless, the transition to online learning brought about its own set of challenges. A notable 35% of respondents, including those who strongly agreed, found interactions among students and between students and teachers to be more uncomfortable and challenging compared to the pre-COVID-19 era. Furthermore, half of the respondents (50%) perceived a decline in on-site class etiquette from pre-pandemic times, reflecting the broader societal shift in norms and behaviors in the wake of remote learning. The findings of this study have the potential to contribute valuable insights into the challenges and opportunities associated with transitioning between different learning modalities and may inform strategies for enhancing students' preparedness and resilience in future educational disruptions.
Differential Gene Profiling of Epstein-Barr Virus and Human Endogenous Retrovirus in Peripheral Blood Mononuclear Cells of Patients with Systemic Lupus Erythematosus: Implications for Immune Response
Authors: Lemus Yesit Bello , Martínez Gustavo Aroca , Lugo Lisandro Pacheco , Escorcia Lorena Gómez , Peñata Eloína Zarate , Llanos Nataly Solano , Bonfanti Andres Cadena , Acosta-Hoyos Antonio J. , Quiroz Elkin Navarro 
Published: June 2024

Abstract

Systemic lupus erythematosus (SLE) is a multifactorial disease characterized by the convergence of genetic, immunological, and viral elements resulting in a complex interaction of both internal and external factors. Research has recognized the role that play the Epstein-Barr virus (EBV) and Human endogenous retrovirus (HERV-E) as triggers and maintenance elements in the disease. A fundamental study area stands out in the dynamics between these viral agents and their physiopathology to unveil their influence in SLE development and progress. This study aimed at assessing the differential expression of immune regulatory genes and the incidence of specific viral pathogens (EBV and HERV-E), alongside the detailed characterization of surface markers in T- and B-lymphocytes in patients with SLE and control participants. A comparative analysis between patients with SLE and control participants was performed, evaluating the expression of phenotypic markers and genes involved in the immune response (TNF-α, IL-2, IL-6, IL-10, IFNG, TLR3), as well as HERV-E gag and EBV viral genes (LMP1 and BZLF1). A significant association between SLE and EBV was found in this study, with a marked increase in EBV LMP1 gene expression and a marked reduction in IFN-γ levels in patients with SLE. Also, a significant overexpression of HERV-E was observed, in addition to a considerable increase in the distribution of the cell surface marker CD27 + on T- and B-lymphocytes, observed in individuals with SLE compared to the control group. This study provides evidence regarding the role that EBV virus plays in lymphocytes in the context of SLE, highlighting how both the virus and the host gene expression may influence disease pathogenesis by altering immune regulatory pathways mediated by TNF-α, IFN-γ, and IL-10, as well as parallel overexpression of HERV-E gag.