bluuebunny/crossref_metadata_2025_split huggingface dataset:
Efficacy of iontophoresis with glycopyrronium bromide for treatment of primary palmar hyperhidrosis
Authors: Chia H.Y. , Tan A.S.L. , Chong W.S. , Tey H.L. 
Published: August 2011

AbstractBackground  There is limited data on the efficacy of iontophoretic treatment of primary palmar hyperhidrosis using glycopyrronium bromide. The first line treatment for primary palmar hyperhidrosis is usually topical aluminium chloride, but clinical experience indicates that it is not effective for more severe disease.Objective  To evaluate the efficacy of using glycopyrronium bromide iontophoresis in the treatment of primary palmar hyperhidrosis, and to evaluate if the benefit of treatment varies with the severity of disease.Methods  This is an open‐label study involving patients undergoing weekly treatment of iontophoresis with glycopyrronium bromide for 4 weeks. Gravimetric measurements of sweat production and subjective scores of palmar sweatiness were recorded prior to starting treatment and 1 week after the last treatment. Side‐effects were monitored weekly.Results  Twenty two of the 25 patients recruited completed the 4‐week treatment. There was a significant mean improvement of 23.4 mg/min (P = 0.001) between baseline and post‐treatment gravimetric measurements. Patients with a higher baseline sweat output demonstrated a trend towards a greater reduction in sweat production (Pearson’s correlation correlation coefficient, r = 0.41). The patients experienced dryness of the palms for a mean duration of 5 days after iontophoresis. All patients reported an improvement in satisfaction scores and 81.8% reported an improvement in subjective severity scores. No serious side‐effects were encountered during the study.Conclusions  Iontophoresis using glycopyrronium bromide is an effective and well‐tolerated treatment for primary palmar hyperhidrosis. The possibility of its greater benefit in patients with more severe baseline disease requires verification.
Mild to moderate hidradenitis suppurativa treated with local excision and primary closure
Authors: van Rappard D.C. , Mooij J.E. , Mekkes J.R. 
Published: August 2011

AbstractBackground  Hidradenitis suppurativa (HS) is a chronic skin disease with a great impact on quality of life. Treatment with antibiotics or anti‐inflammatory drugs, such as prednisone or TNF‐alpha‐inhibitors usually achieves only temporary improvement. Surgical intervention is considered as the only curative treatment for recurrent lesions.Objective  To determine the efficacy and patient satisfaction of local excision followed by primary closure.Methods  Between 2005 and 2010, 92 local excisions with primary closure were performed in 57 patients with mild to moderate HS. All patients were treated on an outpatient basis, under local anaesthesia. Local excision was defined as complete excision of the affected tissue, beyond the borders of activity, leaving clear margins. The medical records were reviewed retrospectively in 2010. The final outcome of the procedure, cosmetic appearance and patient satisfaction was measured using a questionnaire.Results  Successful treatment, without recurrence, was accomplished in 66% of the cases. The intervention was generally well tolerated: 84% of the patients stated that they would undergo the same surgical procedure again if necessary, and 89% would recommend the procedure to other patients.Conclusion  Local excision followed by primary closure is a valuable treatment for patients with mild to moderate HS (Hurly stage I & II), with low morbidity and a high patient satisfaction rate.
The effects of online shopping attributes on satisfaction–purchase intention link: a longitudinal study
Authors: Ha Hong‐Youl 
Published: August 2011

AbstractAttributes of online satisfaction are dynamic. Specifically, it remains to be examined how online shopping attributes empirically affect changes in satisfaction that leads to repurchase behaviours over time and why. The present study develops a theoretical framework for conceptualizing satisfaction with temporal effects and empirically tests it using longitudinal data from 219 online customers. The results indicate that the relationship between attribute‐level evaluations and satisfaction, which has only been examined in terms of predictors of satisfaction in prior literature, is dynamic and changes over time. Changed attributes (particularly convenience and site design) might signal an opportunity to capture the dynamics of attribute‐level evaluations over time. Furthermore, the findings reveal significant temporal effects of satisfaction and purchase intention at time point T on satisfaction and purchase intention at time point T + 1. The results suggest that temporal effects occur as a diagnostic function of a previous attribute rating of a subsequent satisfaction evaluation and the consumer's level of subjective knowledge.
Estimation of Simulated Phosphene Size Based on Tactile Perception
Authors: Lu Yanyu , Chen Panpan , Zhao Ying , Shi Jingru , Ren Qiushi , Chai Xinyu 
Published: August 2011

AbstractClinical trials have successfully shown that a visual prosthesis can elicit visual perception (phosphenes) in the visual field. Psychophysical studies based on simulated prosthetic vision offer an effective means to evaluate and refine prosthetic vision. We designed three experiments to examine the effect of phosphene luminance, flicker rate, and eccentricity on the ability to estimate simulated phosphene sizes using tactile perception. Thirty subjects participated in the three experiments. There was a linear increase in reported size as visual stimulus size increased. Judgment was significantly affected by stimulus luminance and eccentricity (P < 0.05) but not by flicker rates. Brighter stimuli were perceived as being larger, and the more eccentric the position, the larger the estimated size. These simulation studies, although idealized, suggested that tactile perception is a potential way to estimate phosphene sizes.
Flow cytometry evaluation of red blood cells mimicking naturally occurring ABO subgroups after modification with variable amounts of function‐spacer‐lipid A and B constructs
Authors: Hult Annika K. , Frame Tom , Chesla Scott , Henry Stephen , Olsson Martin L. 
Published: August 2011

BACKGROUND: Kodecytes bearing synthetic blood group A and B antigens are increasingly being used in transfusion laboratories as serologic mimics of red blood cell (RBC) Aweak and Bweak subtypes. The aim of this study was to compare the flow cytometry profile of kodecytes with native ABO subgroups.STUDY DESIGN AND METHODS: A series of A/B kodecytes, each with decreasing A or B antigen expression, were prepared from group O RBCs that were modified with dilutions of function‐spacer‐lipid KODE technology (FSL) constructs representing a wide serologic range. Using an established flow cytometry method designed for the detection of low levels of A/B antigens, kodecyte profiles were compared with those of native subgroup cells.RESULTS: Kodecytes with positive tube serology from 4+ to 1+ were created with 15 to 2 µg/mL FSL‐A or 78 to 10 µg/mL FSL‐B transformation solutions. The kodecytes created with higher concentrations of FSL constructs revealed a uniform and/or even distribution of antigens as seen by a single flow cytometry peak more narrow than the broader peaks produced with lower FSL concentrations similar to those found in native Ax and most Bweak subgroups.CONCLUSIONS: Although kodecytes are created artificially, they can be designed to mimic the serologic and flow cytometric profiles of native ABO subgroup RBCs.
Implant‐guided vertical bone growth in the mini‐pig
Authors: Freilich Martin , Wen Bo , Shafer David , Schleier Peter , Dard Michel , Pendrys David , Ortiz Denise , Kuhn Liisa 
Published: August 2011

AbstractObjective: To attain and describe guided vertical bone regeneration around titanium (Ti) and titanium zirconium (Ti–Zr) dental implants utilizing non‐glycosylated recombinant human bone morphogenetic protein‐2 (ng/rhBMP‐2), biomaterial scaffolds and a scaffold retainer.Materials and methods: Thirty‐two modified Straumann TE implants were partially embedded in the mandibles of eight adult mini‐pigs. Pre‐shaped resorbable scaffolds were placed around the implant and shielded and stabilized with a newly developed Ti custom scaffold retainer (umbrella) or wide‐neck (WN) healing caps to stabilize the scaffold. Ng/rhBMP‐2 (50 μg) was applied to the supracrestal portion of the implant or incorporated within the scaffold. At 9 weeks, soft tissue healing was assessed. Vertical bone regeneration outcomes including bone height, bone‐to‐implant contact (BIC) and bone volume were assessed by micro‐computed tomography and histology.Results: Soft tissue healing at the test sites (+ng/rhBMP‐2/+scaffold) appeared to be substantially better than the control sites (−ng/rhBMP‐2/−scaffold). Bone height, BIC percentage and bone volume were all similar regardless of whether WN healing caps or umbrella scaffold stabilization was used for all biomaterial scaffolds tested. WN healing cap test sites showed greater new bone height and BIC as compared with aggregate data from the control sites (P=0.05). Comparison of aggregate data from the umbrella test sites showed greater BIC and new bone volume as compared with aggregate data from the control sites(P=0.05).Conclusion: Vertical bone regeneration was successfully attained utilizing ng/rhBMP‐2, biomaterial scaffolds and a scaffold retainer.
Clinical and microbiological characteristics of peri‐implantitis cases: a retrospective multicentre study
Authors: Charalampakis G. , Leonhardt Å. , Rabe P. , Dahlén G. 
Published: August 2011

AbstractObjectivesThe aim of this study was to follow patient cases retrospectively in a longitudinal manner from the time of implant placement to the time they were diagnosed with peri‐implant disease, and to identify associated clinical and microbiological features of peri‐implant disease.Material and methodsA total of 281 patient cases were chosen from the archives of the Oral Microbiological Diagnostic Laboratory, Gothenburg, Sweden, based on bacterial samples taken from diseased implants. A form was designed and filled in separately for each case including data on patient, implant and disease profile.ResultsMost cases were severe peri‐implantitis cases (91.4%). In 41.3% of the patients, peri‐implantitis was developed early, already after having implants in function less than 4 years. The type of implant surface was significantly associated with the time in years implants were in function, before disease was developed (P < 0.05). The microbiological results by both culture and checkerboard analysis, although failed to fully correspond to the severity of the disease in terms of magnitude, proved to show that peri‐implantitis is a polymicrobial anaerobic infection with increased number of AGNB (aerobic Gram‐negative bacilli) in 18.6% of the patients.ConclusionsPeri‐implantitis is a biological complication of implants in function that poses a threat to their long‐term survival. It may develop earlier around implants with rough surfaces and it may represent a true infection. Microbiological sampling methods should be improved and uniformed so as to fully unveil the microbiological profile of the disease.
Cloning and molecular characterization of a novel acyl‐CoA:diacylglycerol acyltransferase 1‐like gene (PtDGAT1) from the diatom Phaeodactylum tricornutum
Authors: Guihéneuf Freddy , Leu Stefan , Zarka Aliza , Khozin‐Goldberg Inna , Khalilov Ilkhom , Boussiba Sammy 
Published: August 2011

We have identified and isolated a cDNA encoding a novel acyl‐CoA:diacylglycerol acyltransferase (DGAT)1‐like protein, from the diatom microalga Phaeodactylum tricornutum (PtDGAT1). The full‐length cDNA sequences of PtDGAT1 transcripts revealed that two types of mRNA, PtDGAT1short and PtDGAT1long, were transcribed from the single PtDGAT1 gene. PtDGAT1short encodes a 565 amino acid sequence that is homologous to several functionally characterized higher plant DGAT1 proteins, and 55% identical to the putative DGAT1 of the diatom Thalassiosira pseudonana, but shows little homology with other available putative and cloned algal DGAT sequences. PtDGAT1long lacks several catalytic domains, owing to a 63‐bp nucleotide insertion in the mRNA containing a stop codon. Alternative splicing consisting of intron retention appears to regulate the amount of active DGAT1 produced, providing a possible molecular mechanism for increased triacylglycerol (TAG) biosynthesis in P. tricornutum under nitrogen starvation. DGAT mediates the last committed step in TAG biosynthesis, so we investigated the changes in expression levels of the two types of mRNA following nitrogen starvation inducing TAG accumulation. The abundance of both transcripts was markedly increased under nitrogen starvation, but much less so for PtDGAT1short. PtDGAT1 activity of PtDGAT1short was confirmed in a heterologous yeast transformation system by restoring DGAT activity in a Saccharomyces cerevisiae neutral lipid‐deficient quadruple mutant strain (H1246), resulting in lipid body formation. Lipid body formation was only restored upon the expression of PtDGAT1short, and not of PtDGAT1long. The recombinant yeast appeared to display a preference for incorporating saturated C16 and C18 fatty acids into TAG.DatabaseNucleotide sequence data are available in the GenBank/EMBL/DDBJ databases under accession number HQ589265, sequence to be released November 15 2011.
INFLUENCE OF DIFFERENT PROCESSING PARAMETERS ON THE ISOLATION OF LUPIN (LUPINUS ANGUSTIFOLIUS L.) PROTEIN ISOLATES: A PRELIMINARY STUDY
Authors: SUSSMANN DANIELA , PICKARDT CLAUDIA , SCHWEIGGERT UTE , EISNER PETER 
Published: August 2011

ABSTRACTProtein isolates were prepared from full‐fat flakes of Lupinus angustifolius L. (cv Vitabor) by salt‐induced extraction followed by precipitation with cold demineralized water. Different process parameters were screened to evaluate their significant influences on the extracted or overall protein yields in lab scale. During the extraction, the factors NaCl concentration, pH value and the ratio of flakes to solvent significantly influenced the protein yields, whereas the extraction time can be neglected in a specific range. During precipitation, the salt concentration, the initial protein concentration, the ratio between extract and water, and finally the sedimentation time had significant effect on the overall protein yield. However, the temperature difference between extract and water as well as the order and speed of addition had no considerable impact on the final protein yield. This study gathered important processing parameters for an efficient protein isolation and their suitable ranges for a subsequent optimization.PRACTICAL APPLICATIONSIn search of viable ways to create more sustainable diets, the application of dietary proteins from plant‐based sources would be an appropriate possibility. Plant proteins, in particular protein isolates, will find an introduction into the world's food system as extenders, supplements and replacements of animal proteins. Currently, the major drawback of protein isolate production is its insufficient economical efficiency including cost‐efficient processing and low recovery yields. Although some important parameters on protein isolation are well established, systematic studies on overall yield including all relevant processing parameters are still scarce. This study identified significant process parameters on the overall protein yield using lupin seed as an example. These findings are a prerequisite for further optimization to obtain plant protein isolates in an efficient and systematic way. This knowledge will facilitate the implementation of plant protein processing on industrial scale and will ensure appropriate amounts of protein isolates with superior quality.
Explained variance for blood gases in a population with COPD
Authors: Saure Eirunn Waatevik , Eagan Tomas Mikal Lind , Jensen Robert Leroy , Voll‐Aanerud Marianne , Aukrust Pål , Bakke Per Sigvald , Hardie Jon Andrew 
Published: August 2011

AbstractIntroduction:  Variation of blood gas levels in chronic obstructive pulmonary disease (COPD) patients has not been extensively reported and there is limited knowledge about predictors of chronic respiratory failure in COPD patients.Objectives:  The aim of this study was to identify predictors of hypoxemia, hypercapnia and increased alveolar‐arterial oxygen difference in COPD patients. We hypothesized that prediction of arterial blood gases will be improved in multivariate models including measurements of lung function, anthropometry and systemic inflammation.Methods:  A cross‐sectional sample of 382 Norwegian COPD patients, age 40–76, Global Initiative for Chronic Obstructive Lung Disease stage II–IV, with a smoking history of at least 10 pack‐years, underwent extensive measurements, including medical examination, arterial blood gases, systemic inflammatory markers, spirometry, plethysmography, respiratory impedance and bioelectrical impedance. Possible predictors of arterial oxygen (PaO2), arterial carbon dioxide (PaCO2) and alveolar‐arterial oxygen difference (AaO2) were analyzed with both bivariate and multiple regression methods.Results:  We found that various lung function measurements were significantly associated with PaO2, PaCO2 and AaO2. In addition, heart rate and Fat Mass Index were predictors of PaO2 and AaO2, while heart failure and current smoking status were associated with PaCO2. The explained variance (R2) in the final multivariate regression models was 0.14–0.20.Conclusions:  With a wide assortment of possible clinical predictors, we could explain 14–20% of the variation in blood gas measurements in COPD patients.Please cite this paper as: Saure EW, Eagan TML, Jensen RL, Voll‐Aanerud M, Aukrust P, Bakke PS and Hardie JA. Explained variance for blood gases in a population with COPD. Clin Respir J 2012; 6: 72–80.
Acute Effects of Nicardipine and Esmolol on The Cardiac Cycle, Intracardiac Hemodynamic and Endothelial Shear Stress in Patients With Unstable Angina Pectoris and Moderate Coronary Stenosis: Results From Single Center, Randomized Study
Authors: Chen Shao‐Liang , Hu Zuo‐Ying , Zhang Jun‐Jie , Ye Fei , Kan Jing , Xu Tian , Liu Zhi‐Zhong , Zhang Yao‐Jun , Zhang Jun‐Xia , Chen Mengxuan 
Published: August 2011

SUMMARYObjective: This study aimed to compare the acute effects of nicardipine and esmolol on hemodynamic and endothelial shear stress (ESS) in patients with unstable angina (UA) and moderate coronary stenosis (MCS). Background: Nicardipine and esmolol exhibit cardioprotection via different mechanisms. However, their acute effects on hemodynamic and ESS are still unknown. Methods: One‐hundred sixteen patients with UA and MSC were randomly divided into nicardipine (n = 59) and esmolol (n = 57) groups. Drugs were injected as a bolus followed by continuous infusion to achieve the steady states defined as the mean blood pressure (MBP) reduced by ≥10% or a heart‐rate change by ≥15 bpm, lasting for at least 10 min. The aortic pressure (AP), EKG, blood velocity, right atrial pressure, distal coronary pressure (DCP), systolic time (ST), isovolumetric diastolic time (IVDT), speed filling time (SFT), and ESS were simultaneously calculated at baseline and steady states. Results: Both drugs significantly reduced blood pressure and rate‐pressure load. Infusion of nicardipine was associated with negative remodeling of the distal segment (P= 0.005). Esmolol, rather than nicardipine, increased minimal lumen diameter (P= 0.040), prolonged SFT (0.34 ± 0.03 s vs. 0.41 ± 0.03 s, P < 0.001), reduced DCP (P < 0.001) and increased blood velocity (33.65 ± 1.07 cm/s vs. 43.36 ± 1.25 cm/s, P < 0.001) at SFT stages, with increased blood‐flow (P < 0.001). Both drugs increased downstream ESS. Esmolol significantly reversed abnormally increased ESS (P < 0.001) and increased upstream ESS compared with nicardipine (P < 0.001). Conclusion: Beyond a similar reduction of AP, patients with UA and MCS could benefit more from the reduction of heart rate induced by esmolol (ChiCTR‐TRC‐10000964).
Lipopolysaccharide (LPS) Inner-Core Phosphates Are Required for Complete LPS Synthesis and Transport to the Outer Membrane in Pseudomonas aeruginosa PAO1
Authors: DeLucia Angela M. , Six David A. , Caughlan Ruth E. , Gee Patricia , Hunt Ian , Lam Joseph S. , Dean Charles R. 
Published: August 2011

ABSTRACT

Gram-negative outer membrane (OM) integrity is maintained in part by Mg
2+
cross-links between phosphates on lipid A and on core sugars of adjacent lipopolysaccharide (LPS) molecules. In contrast to other Gram-negative bacteria,
waaP
, encoding an inner-core kinase, could not be inactivated in
Pseudomonas aeruginosa
. To examine this further, expression of the kinases WaaP or WapP/WapQ
/
PA5006 was placed under the control of the arabinose-regulated pBAD promoter. Growth of these strains was arabinose dependent, confirming that core phosphorylation is essential in
P. aeruginosa
. Transmission electron micrographs of kinase-depleted cells revealed marked invaginations of the inner membrane. SDS-PAGE of total LPS from WaaP-depleted cells showed accumulation of a fast-migrating band. Mass spectrometry (MS) analysis revealed that LPS from these cells exhibits a unique truncated core consisting of two 3-deoxy-
d
-manno-octulosonic acids (Kdo), two
l
-glycero-
d
-manno-heptoses (Hep), and one hexose but completely devoid of phosphates, indicating that phosphorylation by WaaP is necessary for subsequent core phosphorylations. MS analysis of lipid A from WaaP-depleted cells revealed extensive 4-amino-4-deoxy-
l
-arabinose modification. OM prepared from these cells by Sarkosyl extraction of total membranes or by sucrose density gradient centrifugation lacked truncated LPS. Instead, truncated LPS was detected in the inner membrane fractions, consistent with impaired transport/assembly of this species into the OM.

IMPORTANCE
Gram-negative bacteria have an outer membrane (OM) comprised of a phospholipid inner leaflet and a lipopolysaccharide (LPS) outer leaflet. The OM protects cells from toxic molecules and is important for survival during infection. The LPS core kinase gene
waaP
can be deleted in several Gram-negative bacteria but not in
Pseudomonas aeruginosa
. We used a controlled-expression system to deplete WaaP directly in
P. aeruginosa
cells, which halted growth. WaaP depletion also caused gross changes in cell morphology and led to the accumulation of an aberrant LPS lacking several core sugars and all core phosphates. The aberrant LPS failed to reach the OM, suggesting that WaaP is essential in
P. aeruginosa
because it is required to produce the full-length LPS that is recognized by the OM transport/assembly machinery in this organism. Therefore, WaaP may constitute a good target for the development of novel antipseudomonal agents.