bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hematopoietic stem cells exhibit a specific ABC transporter gene expression profile clearly distinct from other stem cells
Authors: Tang Leilei , Bergevoet Saskia M , Gilissen Christian , de Witte Theo , Jansen Joop H , van der Reijden Bert A , Raymakers Reinier AP 
Published: September 2010

Abstract

Background
ATP-binding cassette (ABC) transporters protect cells against unrelated (toxic) substances by pumping them across cell membranes. Earlier we showed that many ABC transporters are highly expressed in hematopoietic stem cells (HSCs) compared to more committed progenitor cells. The ABC transporter expression signature may guarantee lifelong protection of HSCs but may also preserve stem cell integrity by extrusion of agents that trigger their differentiation. Here we have studied whether non-hematopoietic stem cells (non-HSCs) exhibit a similar ABC transporter expression signature as HSCs.

Results
ABC transporter expression profiles were determined in non-hematopoietic stem cells (non-HSCs) from embryonic, neonatal and adult origin as well as in various mature blood cell types. Over 11,000 individual ABC transporter expression values were generated by Taqman Low Density Arrays (TLDA) to obtain a sensitivity comparable with quantitative real-time polymerase chain reactions. We found that the vast majority of transporters are significantly higher expressed in HSCs compared to non-HSCs. Furthermore, regardless their origin, non-HSCs exhibited strikingly similar ABC transporter expression profiles that were distinct from those in HSCs. Yet, sets of transporters characteristic for different stem cell types could be identified, suggesting restricted functions in stem cell physiology. Remarkably, in HSCs we could not pinpoint any single transporter expressed at an evidently elevated level when compared to all the mature blood cell types studied.

Conclusions
These findings challenge the concept that individual ABC transporters are implicated in maintaining stem cell integrity. Instead, a distinct ABC transporter expression signature may be essential for stem cell function. The high expression of specific transporters in non-HSCs and mature blood cells suggests a specialized, cell type dependent function and warrants further functional experiments to determine their exact roles in cellular (patho)physiology.
Genome-scale identification of Soybean BURP domain-containing genes and their expression under stress treatments
Authors: Xu Hongliang , Li Yaxuan , Yan Yueming , Wang Ke , Gao Ya , Hu Yingkao 
Published: September 2010

Abstract

Background
Multiple proteins containing BURP domain have been identified in many different plant species, but not in any other organisms. To date, the molecular function of the BURP domain is still unknown, and no systematic analysis and expression profiling of the gene family in soybean (Glycine max) has been reported.

Results
In this study, multiple bioinformatics approaches were employed to identify all the members of BURP family genes in soybean. A total of 23 BURP gene types were identified. These genes had diverse structures and were distributed on chromosome 1, 2, 4, 6, 7, 8, 11, 12, 13, 14, and 18. Phylogenetic analysis suggested that these BURP family genes could be classified into 5 subfamilies, and one of which defines a new subfamily, BURPV. Quantitative real-time PCR (qRT-PCR) analysis of transcript levels showed that 15 of the 23 genes had no expression specificity; 7 of them were specifically expressed in some of the tissues; and one of them was not expressed in any of the tissues or organs studied. The results of stress treatments showed that 17 of the 23 identified BURP family genes responded to at least one of the three stress treatments; 6 of them were not influenced by stress treatments even though a stress related cis-element was identified in the promoter region. No stress related cis-elements were found in promoter region of any BURPV member. However, qRT-PCR results indicated that all members from BURPV responded to at least one of the three stress treatments. More significantly, the members from the RD22-like subfamily showed no tissue-specific expression and they all responded to each of the three stress treatments.

Conclusions
We have identified and classified all the BURP domain-containing genes in soybean. Their expression patterns in different tissues and under different stress treatments were detected using qRT-PCR. 15 out of 23 BURP genes in soybean had no tissue-specific expression, while 17 out of them were stress-responsive. The data provided an insight into the evolution of the gene family and suggested that many BURP family genes may be important for plants responding to stress conditions.
Analysis of human synovial and bone marrow mesenchymal stem cells in relation to heat-inactivation of autologous and fetal bovine serums
Authors: Nimura Akimoto , Muneta Takeshi , Otabe Koji , Koga Hideyuki , Ju Young-Jin , Mochizuki Tomoyuki , Suzuki Koji , Sekiya Ichiro 
Published: September 2010

Abstract

Background
Though sera are essential for Mesenchymal stem cells (MSCs), the effect of heat-inactivation remains unknown. Autologous human serum is recommended for clinical use; however, it is unclear whether differentiation potentials are maintained. To examine whether heat-inactivation of serum affected the proliferation and whether autologous human serum influenced their multipotentiality.

Methods
After whole blood collection, human synovium and bone marrow were harvested. Nucleated cells were expanded with autologous human serum and FBS.

Results
Heat-inactivation of autologous human serum enhanced proliferation of synovial MSCs. Heat-inactivation of each types of serum didn't affect calcification of synovial MSCs. The induction of calcification increased ALP activity, with the exception of bone marrow MSCs with autologous human serum. For adipogenesis of synovial MSCs, the Oil Red-O positive colony forming efficiency with autologous human serum was similar to or less than that with FBS.

Conclusion
These clarified the processing of human autologous serum and the influence of different sera for differentiation of synovial and bone marrow MSCs.
Studies of Anopheles gambiae s.l (Diptera: Culicidae) exhibiting different vectorial capacities in lymphatic filariasis transmission in the Gomoa district, Ghana
Authors: Amuzu Hilaria , Wilson Michael D , Boakye Daniel A 
Published: September 2010

Abstract

Background
Two lymphatic filariasis endemic communities Mampong and Hwida in Ghana have been regularly monitored for impact on transmission after annual mass drug administration (MDA) with albendazole and ivermectin. After six MDAs even though the ABR for Mampong was 55883/person/year and that of Hwida was 2494/person/year, they both had ATPs of 15.21 infective larvae/person/year. Interestingly the human microfilaraemia levels had reduced significantly from 14% to 0% at Mampong and 12% to 3% at Hwida. In an attempt to understand this anomaly, we collected mosquitoes over a 5-month period using human landing catches to determine the species composition, the number of cibarial teeth, the lengths and widths of the cibarium and the cibarial dome of the vector populations.

Results
Out of 2553 mosquitoes caught at Mampong, 42.6% were An. gambiae s.l. All 280 identified further by PCR were An. gambiae s.s (275 M and 5 S molecular forms). At Hwida, 112 mosquitoes were obtained; 67 (59.8%) were An. gambiae s.l, comprised of 40 (59.7%) An. melas, 24 (35.8%) An. gambiae s.s (17 and 5 M and S molecular forms respectively) and 3 (4.5%) unidentified. The mean number of teeth for An. melas was 14.1 (median = 14, range = 12-15), An. gambiae s.s., 15.7 (median = 15, range = 13-19) M form 15.5 (median = 15 range = 13-19) and S form 16 (median = 16, range 15-17). The observed differences in teeth numbers were significantly different between An. melas and An. gambiae s.s (p = 0.004), and the M form (p = 0.032) and the S form (p = 0.002).

Conclusions
In this study, An. gambiae s.s was the main vector at Mampong and was found to possess significantly more cibarial teeth than An. melas, the principal vector at Hwida. We postulate that the different impact observed after 6 MDAs may be due to An. gambiae s.s exhibiting 'facilitation' at Mampong and at Hwida An. melas the main vector exhibits 'limitation'. Thus it may be necessary to compliment MDA with vector control to achieve interruption of transmission in areas where An. melas may exhibit limitation.
Assessment of myocardial abnormalities in rheumatoid arthritis using a comprehensive cardiac magnetic resonance approach: a pilot study
Authors: Kobayashi Yasuyuki , Giles Jon T , Hirano Masaharu , Yokoe Isamu , Nakajima Yasuo , Bathon Joan M , Lima Joao AC , Kobayashi Hitomi 
Published: September 2010

Abstract
Introduction
Rheumatoid arthritis (RA) is a multi-organ inflammatory disorder associated with high cardiovascular morbidity and mortality. We sought to assess cardiac involvement using a comprehensive cardiac magnetic resonance imaging (cMRI) approach and to determine its association with disease characteristics in RA patients without symptomatic cardiac disease.

Methods
RA patients with no history and/or clinical findings of systemic or pulmonary hypertension, coronary artery disease, severe valvular heart disease, atrial fibrillation, diabetes mellitus, or echocardiographic abnormalities underwent contrast-enhanced cMRI on a 1.5T scanner. Adenosine triphosphate was used to assess perfusion defects due to microvascular impairment or ischemia, and delayed enhanced imaging was obtained for the assessment of myocardial inflammation/fibrosis. We explored the associations of cMRI abnormalities with RA disease activity and severity measures.

Results
Eighteen patients (78% female) with a mean age of 57 ± 10 years were studied. Eight patients (45%) demonstrated a myocardial abnormality. Perfusion defects under pharmacologic stress were seen in two patients (11%), one of whom had a circumferential subendocardial perfusion defect and one had a non-segmental subendocardial perfusion defect. Seven patients (39%) were found to have delayed enhancement, only one of whom also demonstrated a perfusion defect. Mean disease activity score (DAS)28 was significantly higher in the group with delayed enhancement compared to the group without by an average of 1.32 DAS28 units (4.77 vs. 3.44 units, respectively; P = 0.011). Corresponding trends to statistical significance were noted in systemic inflammatory markers, with both C-reactive protein (CRP) and erythrocyte sedimentation rate (ESR) quantitatively higher in the group with delayed enhancement. Other RA characteristics, such as disease duration, autoantibody status, and current treatments were not significantly associated with cardiac involvement.

Conclusions
Myocardial abnormalities, as detected by cMRI, were frequent in RA patients without known cardiac disease. Abnormal cMRI findings were associated with higher RA disease activity, suggesting a role for inflammation in the pathogenesis of myocardial involvement in RA.
The pregnancy hormones human chorionic gonadotropin and progesterone induce human embryonic stem cell proliferation and differentiation into neuroectodermal rosettes
Authors: Gallego Miguel J , Porayette Prashob , Kaltcheva Maria M , Bowen Richard L , Vadakkadath Meethal Sivan , Atwood Craig S 
Published: September 2010

Abstract
Introduction
The physiological signals that direct the division and differentiation of the zygote to form a blastocyst, and subsequent embryonic stem cell division and differentiation during early embryogenesis, are unknown. Although a number of growth factors, including the pregnancy-associated hormone human chorionic gonadotropin (hCG) are secreted by trophoblasts that lie adjacent to the embryoblast in the blastocyst, it is not known whether these growth factors directly signal human embryonic stem cells (hESCs).

Methods
Here we used hESCs as a model of inner cell mass differentiation to examine the hormonal requirements for the formation of embryoid bodies (EB's; akin to blastulation) and neuroectodermal rosettes (akin to neurulation).

Results
We found that hCG promotes the division of hESCs and their differentiation into EB's and neuroectodermal rosettes. Inhibition of luteinizing hormone/chorionic gonadotropin receptor (LHCGR) signaling suppresses hESC proliferation, an effect that is reversed by treatment with hCG. hCG treatment rapidly upregulates steroidogenic acute regulatory protein (StAR)-mediated cholesterol transport and the synthesis of progesterone (P4). hESCs express P4 receptor A, and treatment of hESC colonies with P4 induces neurulation, as demonstrated by the expression of nestin and the formation of columnar neuroectodermal cells that organize into neural tubelike rosettes. Suppression of P4 signaling by withdrawing P4 or treating with the P4-receptor antagonist RU-486 inhibits the differentiation of hESC colonies into EB's and rosettes.

Conclusions
Our findings indicate that hCG signaling via LHCGR on hESC promotes proliferation and differentiation during blastulation and neurulation. These findings suggest that trophoblastic hCG secretion and signaling to the adjacent embryoblast could be the commencement of trophic support by placental tissues in the growth and development of the human embryo.
Place of Death: Correlations With Quality of Life of Patients With Cancer and Predictors of Bereaved Caregivers' Mental Health
Authors: Wright Alexi A. , Keating Nancy L. , Balboni Tracy A. , Matulonis Ursula A. , Block Susan D. , Prigerson Holly G. 
Published: September 2010

Purpose To determine whether the place of death for patients with cancer is associated with patients' quality of life (QoL) at the end of life (EOL) and psychiatric disorders in bereaved caregivers. Patients and Methods Prospective, longitudinal, multisite study of patients with advanced cancer and their caregivers (n = 342 dyads). Patients were followed from enrollment to death, a median of 4.5 months later. Patients' QoL at the EOL was assessed by caregiver report within 2 weeks of death. Bereaved caregivers' mental health was assessed at baseline and 6 months after loss with the Structured Clinical Interview for Diagnostic and Statistical Manual of Mental Disorders, Fourth Edition, and the Prolonged Grief Disorder interview. Results In adjusted analyses, patients with cancer who died in an intensive care unit (ICU) or hospital experienced more physical and emotional distress and worse QoL at the EOL (all P ≤ .03), compared with patients who died at home with hospice. ICU deaths were associated with a heightened risk for posttraumatic stress disorder, compared with home hospice deaths (21.1% [four of 19] v 4.4% [six of 137]; adjusted odds ratio [AOR], 5.00; 95% CI, 1.26 to 19.91; P = .02), after adjustment for caregivers' preexisting psychiatric illnesses. Similarly, hospital deaths were associated with a heightened risk for prolonged grief disorder (21.6% [eight of 37] v 5.2% [four of 77], AOR, 8.83; 95% CI, 1.51 to 51.77; P = .02), compared with home hospice deaths. Conclusion Patients with cancer who die in a hospital or ICU have worse QoL compared with those who die at home, and their bereaved caregivers are at increased risk for developing psychiatric illness. Interventions aimed at decreasing terminal hospitalizations or increasing hospice utilization may enhance patients' QoL at the EOL and minimize bereavement-related distress.
Novel benzopyranopyridine derivatives of 2-amino-3-formylchromone
Authors: Siddiqui Zeba , Praveen Shagufta , Farooq Farheen 
Published: September 2010

AbstractEnol lactones such as 4-hydroxy-6-methyl-2H-pyran-2-one (triacetic acid lactone, TAL) and 4-hydroxycoumarin when treated with 2-amino-3-formylchromone under basic conditions afforded 3-acetoacetyl benzopyranopyridones and benzopyranopyridines, respectively. A series of pyrazole derivatives was prepared by the reaction of 3-acetoacetyl benzopyranopyridones with different hydrazines. All compounds were characterised on the basis of spectral data and their antibacterial activity evaluated.
Thermo-physical properties of silver/carbon fibre composites
Authors: Edtmaier Christian , Hula Robert Christian 
Published: September 2010

AbstractSilver matrix composites with short and long carbon fibres of high intrinsic thermal conductivity were fabricated either by liquid metal infiltration or electroless deposition of the Ag matrix and subsequent hot pressing. Active elements enhancing the bonding between inclusions and matrix were introduced by pre-conditioning the fibres thus to influence and improve the overall thermo-physical properties of the composites. The evolution of the composite thermal conductivity (κc) and the coefficient of thermal expansion (αc) as a function of the fibre volume fraction, fibre type, fibre length (fibre orientation) and the presence of active elements such as cobalt, molybdenum, nickel, tungsten and boron are presented in detail and compared with different analytical models.
Influence of reinforcement contiguity on the thermal expansion of alumina particle reinforced aluminium composites
Authors: Roudini Ghodratollah , Tavangar Reza , Weber Ludger , Mortensen Andreas 
Published: September 2010

AbstractPore-free composites combining alumina particles within a matrix of pure aluminium are produced by pressure infiltration, varying systematically the degree of the contiguity, β, of the ceramic phase by sintering prior to infiltration. The thermal expansion of these composites is characterized using repeated cycling in a dilatometer from 303 to 543 K. The initial composite thermal expansion measured immediately after changing the sign of the temperature–time function conforms with predictions from thermoelasticity. Upon further temperature excursion, upward deviations from thermoelastic predictions are found; these are attributed to the influence of matrix plasticity, the influence of which decreases as β increases. The composite thermal expansion travels across 80 % of the theoretical range of initial thermoelastic values as β increases from 0 to 0.123, leading to the conclusion that even a small level of connectivity of the ceramic phase causes significant changes in the thermal expansion of isotropic metal/ceramic composites.
Experimental investigation of thermal fatigue behaviour of header tube to stub welded joint in power plants
Authors: Jinu G. R. , Sathiya P. , Ravichandran G. , Rathinam A. 
Published: September 2010

Abstract
Super heater tubes and outlet headers are subjected to thermal fatigue from alternating heating and cooling during start up and shut down, causing cracks and eventually failure. Super heater tubes are subjected to a temperature of 600 °C and steam at a temperature of 540 °C and a pressure of 160 bar. In this paper, a laboratory simulation for reproducing thermal fatigue phenomena was developed to determine the number of cycles before failure occurs in a header tube to stub welded joint. For this purpose a carbon steel tube (stub) welded to a carbon steel plate which resembles a header pipe to stub welded joint was subjected to thermal cycles from 800 °C (accelerated test) to room temperature. A tungsten inert gas arc was used as a heating source and a water bath was utilized for quenching. After 300 thermal cycles, the cross-section of the header was subjected to optical microscopy. Micro fissures were observed in the header tube. This study reveals that subsequent localized heating and cooling will cause thermal fatigue, which will initiate and propagate cracks in the tubes.
Uncemented Total Hip Arthroplasty with a Tapered Femoral Component: A 22- to 26-Year Follow-Up Study
Authors: McLaughlin Jeffrey R. , Lee Kyla R. 
Published: September 2010

The goal of this study was to evaluate the outcome at a mean follow-up of 24.5 years (range, 22–26 years) of a consecutive series of 138 patients (145 hips) treated with total hip arthroplasty (THA) with the use of the Taperloc femoral component (Biomet, Warsaw, Indiana). This was an FDA-approved prospective study. All surgeries were performed by a single surgeon. Each of the 138 patients (145 hips) was followed annually until death, femoral component revision, or a minimum of 22 years. Complete clinical and radiographic follow-up was obtained on 95% of living patients at 5 years, and 100% at 10, 15, 20, and 24.5 years. Of the original series of 145 hips, only 1 femoral component (0.7%) had undergone revision for aseptic loosening, and 1 was loose by radiographic criteria (0.7%). Femoral osteolysis was identified in 8 hips (5.5%).
A subset of 63 THAs in 56 patients was followed for a mean of 24.5 years. In this group, no femoral component required revision for aseptic loosening, and 1 was loose by radiographic criteria. Femoral osteolysis was present in 4 hips. Survivorship analysis of the femoral component with revision for aseptic loosening as the endpoint was 99% (95% confidence interval, 0.97–100) at 26 years.
This stem demonstrates excellent fixation at a follow-up of 24.5 years with no apparent deterioration of the results with time.
Optimal Amount of Anisometropia for Pseudophakic Monovision
Authors: Hayashi Ken , Yoshida Motoaki , Manabe Shin-ichi , Hayashi Hideyuki 
Published: September 2010

Purpose:
To determine the optimal target anisometropia for pseudophakic monovision.

Methods:
Thirty-five bilaterally pseudophakic patients who received monofocal intraocular lenses were included in the study. Binocular corrected distance visual acuity (CDVA) and binocular distance-corrected near visual acuity (DCNVA) and stereoacuity were measured after simulating 1.00, 1.50, and 2.00 diopters (D) of monovision by adding the appropriate spherical lens to the nondominant eye. We presumed that mean binocular DCNVA of 20/40, binocular CDVA of 20/25, and stereoacuity <100 seconds of arc (arc sec) were necessary for successful monovision.

Results:

With no anisometropia, mean binocular DCNVA was 20/97, binocular CDVA was 20/20, and mean stereoacuity was 71 arc sec. With 1.00 D of monovision, mean binocular DCNVA was only 20/60, although binocular CDVA and mean stereoacuity were sufficient. With 1.50 D of monovision, binocular DCNVA was 20/38, binocular CDVA at other distances exceeded 20/21, and stereoacuity was 100 arc sec, which was a 29-arc sec reduction. With 2.00 D of monovision, binocular DCNVA reached 20/31, but stereoacuity was 158 arc sec, which was an 87-arc sec reduction. The number of patients who met the criteria for successful monovision was significantly greater with 1.50 D of monovision than with 1.00 or 2.00 D of monovision (
P
=.0134).

Conclusions:
Pseudophakic monovision with anisometropia of 1.50 or 2.00 D provides useful binocular visual acuity from far to near. However, because stereopsis with 2.00 D of monovision is substantially impaired, approximately 1.50 D of anisometropia is thought to be optimal for successful monovision.