bluuebunny/crossref_metadata_2025_split huggingface dataset:
Microbiological evaluation of the efficacy of two new biodetergents on multidrug-resistant nosocomial pathogens
Authors: Liguori Giorgio , Bagattini Maria , Gallè Francesca , Quartucci Valeria , Di Onofrio Valeria , Negrone Mario , Triassi Maria 
Published: December 2009

Abstract

Background
In the last few years, several outbreaks of nosocomial infections caused by multidrug-resistant pathogenic agents have been observed, and various biocides products were developed in order to control this phenomenon. We investigated the efficacy of two natural biodetergents composed of plants and kelps extracts, BATT1 and BATT2, against multidrug-resistant strains.

Methods

In-vitro antibacterial efficacy of BATT1 and BATT2 against nosocomial multidrug-resistant isolates was assessed using a suspension-inhibition test, with and without bovine serum albumin (BSA). The test was also carried out on glass surfaces with and without BSA.

Results

In vitro tests with both biocidal disinfectants at 25% concentration demonstrated an overall drop in bacterial, mould and yeast counts after 10 min of contact with or without organic substances. For Pseudomonas aeruginosa, it was necessary to use undiluted disinfectants with and without an organic substance. The same results were obtained in tests carried out on glass surfaces for all strains.

Conclusions
The natural products BATT1 and BATT2 behave like good biocides even in presence of organic substances. The use of both disinfectants may be beneficial for reducing hospital-acquired pathogens that are not susceptible to disinfectants.
However, it has to be stressed that all these experiments were carried out in vitro and they still require validation from use in clinical practice.
Circulating endothelial progenitor cells: a new approach to anti-aging medicine?
Authors: Mikirova Nina A , Jackson James A , Hunninghake Ron , Kenyon Julian , Chan Kyle WH , Swindlehurst Cathy A , Minev Boris , Patel Amit N , Murphy Michael P , Smith Leonard , Alexandrescu Doru T , Ichim Thomas E , Riordan Neil H 
Published: December 2009

AbstractEndothelial dysfunction is associated with major causes of morbidity and mortality, as well as numerous age-related conditions. The possibility of preserving or even rejuvenating endothelial function offers a potent means of preventing/treating some of the most fearful aspects of aging such as loss of mental, cardiovascular, and sexual function.Endothelial precursor cells (EPC) provide a continual source of replenishment for damaged or senescent blood vessels. In this review we discuss the biological relevance of circulating EPC in a variety of pathologies in order to build the case that these cells act as an endogenous mechanism of regeneration. Factors controlling EPC mobilization, migration, and function, as well as therapeutic interventions based on mobilization of EPC will be reviewed. We conclude by discussing several clinically-relevant approaches to EPC mobilization and provide preliminary data on a food supplement, Stem-Kine, which enhanced EPC mobilization in human subjects.
Base composition, selection, and phylogenetic significance of indels in the recombination activating gene-1 in vertebrates
Authors: Chiari Ylenia , van der Meijden Arie , Madsen Ole , Vences Miguel , Meyer Axel 
Published: December 2009

Abstract

Background
The Recombination Activating Proteins, RAG1 and RAG2, play a crucial role in the immune response in vertebrates. Among the nuclear markers currently used for phylogenetic purposes, Rag1 has especially enjoyed enormous popularity, since it successfully contributed to elucidating the relationships among and within a large variety of vertebrate lineages. We here report on a comparative investigation of the genetic variation, base composition, presence of indels, and selection in Rag1 in different vertebrate lineages (Actinopterygii, Amphibia, Aves, Chondrichthyes, Crocodylia, Lepidosauria, Mammalia, and Testudines) through the analysis of 582 sequences obtained from Genbank. We also analyze possible differences between distinct parts of the gene with different type of protein functions.

Results
In the vertebrate lineages studied, Rag1 is over 3 kb long. We observed a high level of heterogeneity in base composition at the 3rd codon position in some of the studied vertebrate lineages and in some specific taxa. This result is also paralleled by taxonomic differences in the GC content at the same codon position. Moreover, positive selection occurs at some sites in Aves, Lepidosauria and Testudines. Indels, which are often used as phylogenetic characters, are more informative across vertebrates in the 5' than in the 3'-end of the gene. When the entire gene is considered, the use of indels as phylogenetic character only recovers one major vertebrate clade, the Actinopterygii. However, in numerous cases insertions or deletions are specific to a monophyletic group.

Conclusions

Rag1 is a phylogenetic marker of undoubted quality. Our study points to the need of carrying out a preliminary investigation on the base composition and the possible existence of sites under selection of this gene within the groups studied to avoid misleading resolution. The gene shows highly heterogeneous base composition, which affects some taxa in particular and contains sites under positive selection in some vertebrate lineages in the 5'-end. The first part of the gene (5'-end) is more variable than the second (3'-end), and less affected by a heterogeneous base composition. However, in some vertebrate lineages the 5'-end of the gene is not yet widely used for phylogenetic studies.
Lipoid pneumonia presenting as non resolving community acquired pneumonia: a case report
Authors: Hadda Vijay , Khilnani Gopi C , Bhalla Ashu S , Mathur Sandeep 
Published: December 2009

Abstract

Introduction
Lipoid pneumonia is a rare form of pneumonia caused by inhalation or aspiration of fat containing substances like, petroleum jelly, mineral oils, few laxatives etc. It usually presents as insidious onset chronic respiratory illness simulating interstitial lung diseases. Rarely, it may present as an acute respiratory illness, specially, when exposure to fatty substance is acute and/or massive. Radiologically, it may mimic carcinoma, acute or chronic pneumonia, ARDS, or a localized granuloma. Diagnosis of LP requires demonstration of lipid laden macrophages in sputum, bronchoalveolar lavage fluid or fine needle aspiration cytology/biopsy from lung lesion. Treatment of this illness is poorly defined and constitutes supportive therapy and corticosteroids.

Case presentation
A 20-year old Indian farmer was referred to us with a diagnosis of non resolving community acquired pneumonia. Respiratory examination revealed signs of consolidation. Chest radiograph revealed findings suggestive of bilateral consolidation. Sputum and blood culture were sterile. He was treated with prolonged course of various antibiotics without any significant response. For evaluation of non resolving pneumonia fibreoptic bronchoscopy was done. Bronchoalveolar lavage fluid and biopsy from lung lesion showed lipid laden macrophages. Hence diagnosis of lipoid pneumonia was made. Patient was treated with course of corticosteroids with good response. Literature on this rare entity is discussed.

Conclusion
Lipoid pneumonia is a rare form of pneumonia which rarely present acutely resembling community acquired pneumonia and requires high degree of suspicion for diagnosis. Its treatment is difficult and poorly defined. However, prolonged corticosteroids may be effective.
Immunoglobulin G galactosylation and sialylation are associated with pregnancy-induced improvement of rheumatoid arthritis and the postpartum flare: results from a large prospective cohort study
Authors: van de Geijn Fleur E , Wuhrer Manfred , Selman Maurice HJ , Willemsen Sten P , de Man Yaël A , Deelder André M , Hazes Johanna MW , Dolhain Radboud JEM 
Published: December 2009

Abstract
Introduction
Improvement of rheumatoid arthritis (RA) during pregnancy has been causatively associated with increased galactosylation of immunoglobulin G (IgG) N-glycans. Since previous studies were small, did not include the postpartum flare and did not study sialylation, these issues were addressed in the present study.

Methods
Serum from 148 RA cases and 32 healthy controls was collected at several time points before, during and after pregnancy. Improvement during pregnancy and postpartum flare were determined according to the European League Against Rheumatism (EULAR) response criteria. Galactosylation and sialylation of Immunoglobulin G (IgG) and the presence of bisecting N-acetylglucosamine (GlcNAc) were analyzed by matrix-assisted laser desorption/ionization - time of flight - mass spectrometry (MALDI-TOF-MS).

Results
IgG1 and IgG2 galactosylation of the cases and controls increased during pregnancy with a maximum in the third trimester. Galactosylation decreased directly postpartum. IgG galactosylation of controls was at a higher level than cases (P < 0.001 at all time points) and a similar pattern was observed for sialylation. Moreover, there was a good association between galactosylation and sialylation. The increase in galactosylation was significantly more pronounced for cases with improvement than cases without improvement during pregnancy. The reverse was true for deteriorators and non-deteriorators postpartum. The presence of bisecting GlcNAc was not significantly influenced by pregnancy or postpartum for cases and controls.

Conclusions
This large cohort study demonstrates the association of changes in galactosylation with both pregnancy-induced improvement and postpartum flare in RA-patients, suggesting a role for changes in glycosylation in the pregnancy-induced improvement of RA.
OR Practice—Catch-Up Scheduling for Childhood Vaccination
Authors: Engineer Faramroze G. , Keskinocak Pınar , Pickering Larry K. 
Published: December 2009

In this paper, we outline the development of the core optimization technology used within a decision support tool to help providers and caretakers in constructing catch-up schedules for childhood immunization. These schedules ensure that a child continues to receive timely coverage against vaccine-preventable diseases in the likely event that one or more doses have been delayed. This project was undertaken as part of a collaborative effort between the Centers for Disease Control and Prevention (CDC) and Georgia Institute of Technology. Our aim is to develop a decision support tool that removes from the task of constructing catch-up schedules the tedious combinatorial aspects, while maintaining a level of generality that allows easy accommodation for changes in the existing rules and adding new vaccines to the schedule lineup. We show that the catch-up scheduling problem is NP-hard, and we develop a dynamic programming algorithm that exploits the typical size and structure of the problem to construct optimized schedules almost at the click of a button. In using an optimization-based algorithm, our approach is unique not only in methodology but also in the information, strategy, and advice we can offer to the user. The tool is being advocated by both the CDC and the American Academy of Pediatrics (AAP) as a means of encouraging caretakers and providers to take a more proactive role in ensuring timely vaccination coverage for children, as well as ensuring the accuracy and quality of a catch-up regime.
A Clinical Review of Echinocandins in Pediatric Patients
Authors: VandenBussche Heather L , Van Loo Dean A 
Published: December 2009

OBJECTIVE To identify and evaluate available data on pediatric echinocandin use. DATA SOURCES A PubMed search, limited to English-language articles, was conducted (1990-August 2009) using the search terms echinocandin, pediatric, child, pharmacokinetics, caspofungin, micafungin, and anidulafungin. Additional articles were retrieved from citations of selected references. STUDY SELECTION AND DATA EXTRACTION Relevant information on the pharmacology, pharmacokinetics, efficacy, and safety of echinocandins in children was selected. Clinical trials, retrospective reviews, and case series were identified and evaluated. Data from these sources were included in this review. DATA SYNTHESIS Caspofungin is the only echinocandin approved by the Food and Drug Administration for use in children. Pediatric pharmacokinetics has been evaluated with all 3 echinocandins but is limited with anidulafungin. Micafungin is the most well-studied agent in prospective clinical trials for antifungal prophylaxis in stem cell transplantation and treatment of invasive fungal infections. Caspofungin has been studied prospectively for febrile neutropenia and treatment of invasive fungal infections, but most published data are from retrospective reviews or case reports. One case report of anidulafungin for neonatal candidiasis has been published. The role of echinocandins in the management of invasive pediatric fungal infections has expanded. Micafungin and caspofungin are recommended as primary or alternative treatment of candidemia and esophageal or invasive candidiasis, and as salvage therapy for invasive aspergillosis. Micafungin is recommended for neutropenic prophylaxis in stem cell transplantation, while caspofungin may be used in febrile neutropenia as an alternative to azoles. Dosing has been well established for caspofungin only in children 3 months of age and above. Anidulafungin should be avoided in children until more pharmacokinetic and clinical data become available. CONCLUSIONS Further comparative trials are needed to more clearly define the role of echinocandins, either as monotherapy or in combination for difficult-to-treat infections, in the pediatric population.
Plerixafor for Stem Cell Mobilization in Patients with Non-Hodgkin's Lymphoma and Multiple Myeloma
Authors: Choi Hye-Yoon , Yong Chul-Soon , Yoo Bong Kyu 
Published: December 2009

OBJECTIVE To evaluate the literature characterizing the mechanism of action, pharmacokinetics, pharmacodynamics, and therapeutic efficacy of plerixafor for hematopoietic stem cell (HSC) mobilization for autologous transplantation in patients with non-Hodgkin's lymphoma (NHL) or multiple myeloma. DATA SOURCES A PubMed search (1966-September 2009) was conducted using the key words plerixafor and AMD3100. Manufacturer's prescribing information was also used. STUDY SELECTION AND DATA EXTRACTION English-language articles were selected and data were extracted with a focus on clinical studies of HSC mobilization in patients with NHL or multiple myeloma. DATA SYNTHESIS Plerixafor exerts its effect by reversibly blocking the ability of HSC to bind to the bone marrow matrix. When used with granulocyte colony-stimulating factor (G-CSF), plerixafor helps increase the number of HSCs in the peripheral blood, where they can be collected for use in autologous transplantation. In clinical studies, plerixafor was rapidly absorbed after subcutaneous injection, reaching a maximum plasma concentration at approximately 0.5 hours. Plerixafor is renally excreted as the parent drug, with an elimination half-life ranging from 3 to 5 hours. Plerixafor increases circulating CD34+ cells in the peripheral blood, with a peak effect about 6–9 hours after subcutaneous administration. An approximate 2- to 3-fold increase in the CD34+ cell count is seen by the first dose of plerixafor after 4 consecutive days of G-CSF treatment. In 2 Phase 3 studies in patients with NHL or multiple myeloma, addition of plerixafor to G-CSF resulted in a higher CD34+ cell collection with fewer apheresis days, but failed to show better graft durability or overall patient survival for up to 12 months of follow-up. CONCLUSIONS Clinical trials have demonstrated that the addition of plerixafor to G-CSF was beneficial for HSC mobilization to peripheral blood for collection and subsequent transplantation in patients with NHL or multiple myeloma. Further studies should assess the benefit of the additive use of plerixafor on clinical outcomes.
Reliability of GaN on Si FETs and MMICs
Authors: Gajewski Donald A. , Nagy Walter , Hanson Allen W. , Johnson J.W. , Linthicum K J 
Published: December 2009

AbstractThis paper reviews the reliability results for the gallium nitride on silicon (GaN-on-Si) technologies for commercial and military communications markets. Two technology platforms have been qualified for volume production: one consisting of discrete heterostructure field effect transistors (HFETs) and the other consisting of HFETs integrated with passive components to form monolithic microwave integrated circuits (MMICs). The technology platform qualifications for volume production have been achieved through intrinsic reliability tests on the active and passive device elements as well as extrinsic reliability tests at the product level. This paper presents reliability results on accelerated life test (ALT), high temperature operating life under DC and RF stress (DC/RF-HTOL), electrostatic discharge (ESD), ramped voltage breakdown, electromigration, temperature cycling, robustness under voltage standing wave ratio (VSWR) mismatch conditions, and diode stability. Degradation and breakdown mechanisms are discussed in relation to material properties reliability. The results show that the HFET and MMIC technology platforms display reliable performance for 20 year product lifetime at worst case operating conditions.
Nanoscale Pattern Formation in Polyelectrolyte Gels
Authors: Jha Prateek K. , Solis Francisco J. , de Pablo Juan J. , Olvera de la Cruz Monica 
Published: December 2009

AbstractPolyelectrolyte (PE) gels exhibit complex phase behavior that includes the existence of nanostructures in poor-solvent conditions. The formation of these inhomogeneous structures is made possible by the competition between the short-range hydrophobic, elastic, and entropic interactions and the long-range electrostatic forces. We develop a theoretical framework that describes the effect of monomer and charge inhomogeneities in PE gels. Numerical calculations performed on a salt-free PE gel with one-dimensional heterogeneities demonstrate the presence of nanophases for a finite range of physical parameters.
A PVA-PCL Bioglass Composite with Potential Implications for Osteochondral Tissue Engineering
Authors: Nair Prabha D , Mukundan Lakshmi M , Nirmal Remya , Mohan Neethu 
Published: December 2009

AbstractA bioglass of composition SiO2 (67.12 mol%), CaO (28.5 mol%), and P2O5 (4.38%) was synthesized and stabilized by a novel technique using ethanol. Bioactive glasses have a wide range of application in the field of biomaterials promoting bone bonding as well as bonding to soft tissue. Earlier our lab developed a novel PVA-PCL semi IPN porous and 3D scaffold that was found to favor chondrogenesis. In the present study, a composite of this polymer and bioglass is prepared by an emulsion freeze-drying process, as a porous 3 dimensional scaffold. The scaffolds were characterized for their physiochemical properties and ability to support cartilage tissue regeneration. The composite scaffolds were observed to be non-cytotoxic. The chondrocytes cells cultured in vitro for a month on the composite scaffolds regenerate cartilaginous tissue, secreting GAGs and collagen in amounts nearly comparable to the amounts on the control PVA-PCL scaffold. The composite scaffold is also biomimetic and bioactive and favors mineralization by forming a hydroxycarbonate apatite layer, when immersed in simulated body fluid for a 14 day period. The PVA-PCL-bioglass composite is hence expected to have potential implications as a scaffold for osteochondral tissue engineering.
New 3D-Biophotopolymers with Selective Surface-cell Interactions for Regenerative Medicine
Authors: Heller Christian , Schwentenwein Martin , Varga Franz , Porodec Maja , Schulz-Siegmund Michaela , Russmueller Guenter , Stampfl Jurgen , Liska Robert 
Published: December 2009

AbstractAdditive Manufacturing Technologies (AMTs) have become an appealing method for the fabrication of 3D cellular scaffolds for tissue engineering and regenerative medicine. To circumvent the use of (meth)acrylate based photopolymers, that suffer from skin irritation and sometimes cytotoxicity, new monomers based on vinyl esters, carbonates and carbamates were prepared. The new materials, giving poly(vinyl alcohol) upon hydrolysis, showed similar results compared to (meth)acrylate references concerning the photoreactivity and mechanical properties, yet being significantly less cytotoxic.To study the kinetics of hydrolytic degradation, the influence of the different polymerizable groups was investigated by hydrolysis of model compounds under alkaline conditions. We were able to show that the ester moiety of a vinyl ester based polymer could be used to immobilize alkaline phosphatase, therefore they exhibit the ability to immobilize enzymes for selective cell adhesion.Finally, 3D test structures by AMT techniques could be fabricated and in-vivo testing thereof proofed the biocompatibility of vinyl ester-based scaffolds.
Increased Vitreous Shedding of Microparticles in Proliferative Diabetic Retinopathy Stimulates Endothelial Proliferation
Authors: Chahed Sadri , Leroyer Aurélie S. , Benzerroug Mounir , Gaucher David , Georgescu Adriana , Picaud Serge , Silvestre Jean-Sébastien , Gaudric Alain , Tedgui Alain , Massin Pascale , Boulanger Chantal M. 
Published: December 2009

OBJECTIVE
Diabetic retinopathy is associated with progressive retinal capillary activation and proliferation, leading to vision impairment and blindness. Microparticles are submicron membrane vesicles with biological activities, released following cell activation or apoptosis. We tested the hypothesis that proangiogenic microparticles accumulate in vitreous fluid in diabetic retinopathy.

RESEARCH DESIGN AND METHODS
Levels and cellular origin of vitreous and plasma microparticles from control (n = 26) and diabetic (n = 104) patients were analyzed by flow cytometry, and their proangiogenic activity was assessed by in vitro thymidine incorporation and neovessel formation in subcutaneous Matrigel plugs in mice.

RESULTS
Microparticles of endothelial, platelet, photoreceptor, and microglial origin were identified in vitreous samples. Levels of photoreceptor and microglial microparticles were undetectable in plasmas but were comparable in diabetic and control vitreous samples. Vitreous platelet and endothelial microparticles levels were increased in diabetic patients and decreased following panretinal laser photocoagulation or intravitreal antivascular endothelial growth factor injection in proliferative diabetic retinopathy (PDR). The ratio of vitreous to plasma microparticle levels was calculated to estimate local formation versus potential plasma leakage. In PDR, the endothelial microparticles ratio—but not that for platelet—was greater than 1.0, indicating local formation of endothelial microparticles from retinal vessels and permeation of platelet microparticles from plasma. Isolated vitreous microparticles stimulated by 1.6-fold endothelial proliferation and increased new vessel formation in mice.

CONCLUSIONS
The present study demonstrates that vitreous fluid contains shed membrane microparticles of endothelial, platelet, and retinal origin. Vitreous microparticles levels are increased in patients with diabetic retinopathy, where they could contribute to disease progression.