bluuebunny/crossref_metadata_2025_split huggingface dataset:
Establishment of a mouse allergy model for Culicoides (Diptera: Ceratopogonidae)
Authors: Bao Shuncai , Lu Xue , Xiang Shuai , Hou Xiaohui 
Published: April 2024

AbstractBackgroundCulicoides is a genus of ubiquitous biting midges (Ceratopogonidae). Female midges have blood‐sucking habit. They not only bite and harass humans and animals but also may be an important vector of disease transmission. Therefore, building an animal allergy model caused by Culicoides biting is very beneficial for studying its pathogenesis and exploring the therapeutic methods.Material and methodKunming mice were used in this study to build the model and sensitised by two‐step injection of midge extracts. Scratching behaviour and histological examination were used to check the immediate and delayed responses. Immunoglobulin E (IgE) and Immunoglobulin G (IgG) were detected using indirect enzyme‐linked immunosorbent assay (ELISA) assay. Splenic cell proliferation and cytokine production were determined using 3‐(4, 5‐dimethylthiazol‐2‐yl)‐2, 5‐diphenyl tetrazolium bromide (MTT) and ELISA assays. The response of cytokine gene expression to midge stimulation was analysed through quantitative real‐time polymerase chain reaction (qPCR).ResultsBehavioural results revealed a significant increase in scratching frequency among the midge‐sensitised animals (p < 0.05). Histological examination showed more inflammatory cytokine infiltration at the injection site of midge‐sensitised mice comparing to the ones in the control group. The serum levels of IgE and IgG1 antibodies in the midge‐sensitised group were significantly elevated (p < 0.05). After splenocytes were stimulated in vitro with midge extracts, the midge‐sensitised group's splenocyte count significantly increased in comparison to the control group. The midge‐sensitised group's qPCR data revealed a down‐regulation of tumor necrosis factor alpha (TNF‐α) expression and an increase in the expression of interleukin (IL)‐4, IL‐5, IL‐10 and IL‐13 but not in the control group (p < 0.05).ConclusionsIn this study, an animal model of Culicoides–mouse sensitisation was successfully constructed using a two‐step method. The mode of administration of the model was in good agreement with the natural immune pathway, and the immune response induced by the sensitisation of the model was similar to that produced by the bite of a midge.
Synthesis and application of environmental plasticizers based on benzene polyacid ester
Authors: Zhu Xiaoyuan , Jiang Pingping , Leng Yan , Lu Minjia , Lv Zhihao , Li Yuchao , Li Zhenhua 
Published: April 2024

AbstractUsing benzene‐1,3,5‐tricarboxylic acid, trimellitic anhydride, 2‐propylheptanol and 2‐ethylhexanol as raw materials, four kinds of environmental plasticizers were prepared, which were used as additives for polyvinyl chloride (PVC) products. Compared with commercially available di (2‐ethylhexyl) phthalate (DOP), di (2‐ethylhexyl) terephthalate (DOTP), and tributyl citrate (TBC), the application performance and plasticizing performance were compared. The structure of the target product was analyzed by fourier transform infrared spectroscopy and nuclear magnetic resonance spectroscopy to verify the fingerprint region of the molecular structure of the product. The properties of plasticized PVC films were tested by thermal weight loss analysis, oven thermal aging test, migration and volatility resistance test, DMA analysis, and scanning electron microscopy. The plasticizing mechanism was confirmed through quantum chemical calculations, and the results of the calculations were in line with the migration outcomes. The results showed that compared with commercially available plasticizers, benzene polyacid ester plasticizers had better thermal stability, and better cold resistance in the polar environments, suitable for high‐temperature and water‐based environments.Highlights
The four plasticizers prepared in this study were compared with reference plasticizers, and the plasticizing mechanism was validated through quantum chemical calculations.
The PVC film plasticized with benzene polyacid ester prepared in this study demonstrates outstanding thermal stability, surpassing certain plasticizers reported in current literature.
Benzene polyacid esters plasticized PVC film has migration stability under various conditions, it has great potential in packaging and other applications.
How to Monitor an Economy’s Contribution to Meeting Basic Needs
Authors: Joffe Michael 
Published: April 2024

AbstractEconomic outcomes are intermediate between economic outputs and their impact on people’s lives. They represent the major way in which economic activity positively impacts health and wellbeing. The monitoring of such intermediate outcomes has useful measurement properties, because they apply to the whole population, they are present early and allow preventive measures to be undertaken, and they directly indicate where intervention is necessary thereby helping to set the policy agenda.A provisional list of possible indicators for high-income countries is presented. The choice of items is based on the literature on human needs, and on the literatures on impact—the social determinants of health, and the emerging evidence on the economic determinants of subjective wellbeing. Data on these items are already collected, although some development work is needed to put them in the required format for the proposed monitoring system. It is desirable that the list of economic outcome measures should be standardised internationally, albeit with separate lists for different levels of economic prosperity.Many items are expressed in terms of “access”, which combines availability and affordability; the criterion is whether or not the need is met, irrespective of how this is achieved. Insecurity is another pervasive issue, because of its impact on people’s quality of life.Each item would be presented as theproportion unfulfilled, the proportion of the population who lack a particular amenity. This metric corresponds to the commitment to leave no one behind, i.e. the value system that everyone’s basic needs should be met, and is readily understood by most people. This implies that an agreed threshold is required for each item. Economic outcomes would be presented as a dashboard for public discussion and policy development, and as the aggregate measure, theIndex of Economic Outcomes(IEO), for the overall evaluation of economic success.The use of the proportion unfulfilled highlights inequalities, at least at the lower end of the income scale, implying that there is no need for a separate measure of inequalities as there is with per capita GDP (and with most other measures). It is compatible with different degrees of inequality higher up the scale, and therefore with a range of political views, implying that it can command wide popular support. An important implication of the proportion unfulfilled metric is that it requires representation of the whole population, including “hard-to-reach” groups.The focus on economic outcomes that meet basic needs while minimising environmental damage corresponds to the perspective of the UN Sustainable Development Goals. The development work involved in establishing the IEO and its component items in the rich world could contribute to future work on the monitoring system for the Goals.
On Homogeneous Spaces for Diagonal Ind-Groups
Authors: Fresse Lucas , Penkov Ivan 
Published: April 2024

AbstractWe study the homogeneous ind-spaces $$\textrm{GL}(\textbf{s})/\textbf{P}$$

GL
(
s
)
/
P

where $$\textrm{GL}(\textbf{s})$$

GL
(
s
)

is a strict diagonal ind-group defined by a supernatural number $$\textbf{s}$$
s
and $$\textbf{P}$$
P
is a parabolic ind-subgroup of $$\textrm{GL}(\textbf{s})$$

GL
(
s
)

. We construct an explicit exhaustion of $$\textrm{GL}(\textbf{s})/\textbf{P}$$

GL
(
s
)
/
P

by finite-dimensional partial flag varieties. As an application, we characterize all locally projective $$\textrm{GL}(\infty )$$

GL
(
∞
)

-homogeneous spaces, and some direct products of such spaces, which are $$\textrm{GL}(\textbf{s})$$

GL
(
s
)

-homogeneous for a fixed $$\textbf{s}$$
s
. The very possibility for a $$\textrm{GL}(\infty )$$

GL
(
∞
)

-homogeneous space to be $$\textrm{GL}(\textbf{s})$$

GL
(
s
)

-homogeneous for a strict diagonal ind-group $$\textrm{GL}(\textbf{s})$$

GL
(
s
)

arises from the fact that the automorphism group of a $$\textrm{GL}(\infty )$$

GL
(
∞
)

-homogeneous space is much larger than $$\textrm{GL}(\infty )$$

GL
(
∞
)

.
A Holistic Approach to Circular Bioeconomy Through the Sustainable Utilization of Microalgal Biomass for Biofuel and Other Value-Added Products
Authors: Ezhumalai Ganesan , Arun Muthukrishnan , Manavalan Arulmani , Rajkumar Renganathan , Heese Klaus 
Published: April 2024

Abstract
Emissions from transportation and industry primarily cause global warming, leading to floods, glacier melt, and rising seas. Widespread greenhouse gas emissions and resulting global warming pose significant risks to the environment, economy, and society. The need for alternative fuels drives the development of third-generation feedstocks: microalgae, seaweed, and cyanobacteria. These microalgae offer traits like rapid growth, high lipid content, non-competition with human food, and growth on non-arable land using brackish or waste water, making them promising for biofuel. These unique phototrophic organisms use sunlight, water, and carbon dioxide (CO2) to produce biofuels, biochemicals, and more. This review delves into the realm of microalgal biofuels, exploring contemporary methodologies employed for lipid extraction, significant value-added products, and the challenges inherent in their commercial-scale production. While the cost of microalgae bioproducts remains high, utilizing wastewater nutrients for cultivation could substantially cut production costs. Furthermore, this review summarizes the significance of biocircular economy approaches, which encompass the utilization of microalgal biomass as a feed supplement and biofertilizer, and biosorption of heavy metals and dyes. Besides, the discussion extends to the in-depth analysis and future prospects on the commercial potential of biofuel within the context of sustainable development. An economically efficient microalgae biorefinery should prioritize affordable nutrient inputs, efficient harvesting techniques, and the generation of valuable by-products.

Graphical Abstract
Synergistic immunomodulatory effect of synbiotics pre- and postoperative resection of pancreatic ductal adenocarcinoma: a randomized controlled study
Authors: Maher Sara , Elmeligy Hesham A. , Aboushousha Tarek , Helal Noha Said , Ossama Yousra , Rady Mahmoud , Hassan Ahmed Mohamed A. , Kamel Manal 
Published: April 2024

AbstractPancreatic ductal adenocarcinoma (PDAC) is an aggressive cancer with a highly immunosuppressive microenvironment. This single-blind, randomized study aimed to evaluate the synergistic immunomodulatory effects of synbiotics (probiotics and inulin prebiotics), as well as their impact on postoperative complications and outcomes, compared to the use of probiotics alone. Ninety patients diagnosed with PDAC were enrolled and randomly assigned into three groups: the placebo group, the probiotics group (receiving a mixture of ten strains of Lactobacillus, Bifidobacterium, and Streptococcus bacteria at a dose of 25 billion CFUs), and the synbiotics group (the same probiotics along with inulin prebiotics). The interventions were administered for 14 days before the surgery and continued for one month postoperatively. Tumor tissue infiltration of CD8 + T cells and the expression of IFN γ were assessed by immunohistochemistry (IHC). Inflammatory cytokines concentrations, including Il 1 B, IL 6, and IL 10, were evaluated as well by ELISA at various time points pre- and postoperative. Furthermore, patients were followed up after the surgery to assess postoperative short-term outcomes. Our results showed a significant elevation of CD8 + T cell proportion and IFN γ expression in the synbiotics group compared to the probiotics group (p = 0.049, p = 0.013, respectively). Inflammatory cytokines showed a significant gradual decrease in the synbiotics group compared to placebo and probiotics-treated groups (p = 0.000 for both). Administration of synbiotics and probiotics significantly decreased the rate of postoperative complications including anastomotic leakage, diarrhea, and abdominal distension (p = 0.032, p = 0.044, p = 0.042, respectively), with a remarkable reduction in bacteremia in the synbiotics group. These results revealed that this synbiotics formulation potentially enhances the immune response and reduces complications associated with surgery.Clinical trial identification: NCT06199752 (27-12-2023)
High core 1β1,3-galactosyltransferase 1 expression is associated with poor prognosis and promotes cellular radioresistance in lung adenocarcinoma
Authors: Chen Yong , Ji Yanyan , Shen Lin , Li Ying , Ren Yue , Shi Hongcan , Li Yue , Wu Yunjiang 
Published: April 2024

Abstract
Purpose
Core 1β1,3-galactosyltransferase 1 (C1GALT1) exhibits elevated expression in multiple cancers. The present study aimed to elucidate the clinical significance of C1GALT1 aberrant expression and its impact on radiosensitivity in lung adenocarcinoma (LUAD).

Methods
The C1GALT1 expression and its clinical relevance were investigated through public databases and LUAD tissue microarray analyses. A549 and H1299 cells with either C1GALT1 knockdown or overexpression were further assessed through colony formation, gamma-H2A histone family member X immunofluorescence, 5-ethynyl-2′-deoxyuridine incorporation, and flow cytometry assays. Bioinformatics analysis was used to explore single cell sequencing data, revealing the influence of C1GALT1 on cancer-associated cellular states. Vimentin, N-cadherin, and E-cadherin protein levels were measured through western blotting.

Results
The expression of C1GALT1 was significantly higher in LUAD tissues than in adjacent non-tumor tissues both at mRNA and protein level. High expression of C1GALT1 was correlated with lymph node metastasis, advanced T stage, and poor survival, and was an independent risk factor for overall survival. Radiation notably upregulated C1GALT1 expression in A549 and H1299 cells, while radiosensitivity was increased following C1GALT1 knockdown and decreased following overexpression. Experiment results showed that overexpression of C1GALT1 conferred radioresistance, promoting DNA repair, cell proliferation, and G2/M phase arrest, while inhibiting apoptosis and decreasing E-cadherin expression, alongside upregulating vimentin and N-cadherin in A549 and H1299 cells. Conversely, C1GALT1 knockdown had opposing effects.

Conclusion
Elevated C1GALT1 expression in LUAD is associated with an unfavorable prognosis and contributes to increased radioresistance potentially by affecting DNA repair, cell proliferation, cell cycle regulation, and epithelial–mesenchymal transition (EMT).
Morphological-metabolic analysis in Streptomyces rimosus microparticle-enhanced cultivations (MPEC)
Authors: Ścigaczewska Anna , Boruta Tomasz , Bizukojć Marcin 
Published: April 2024

AbstractStreptomyces produce a broad spectrum of biologically active molecules such as oxytetracycline and rimocidin, which are widely used in human and animal treatments. microparticle-enhanced cultivation (MPEC) is one of the tools used for Streptomyces bioprocesses intensification by the control of mycelial morphology. In the present work, morphological changes of Streptomyces rimosus caused by the addition of 10 µm talc microparticles in MPEC were correlated with the biosynthetic activity of the microorganism. Comparing the runs with and without microparticles, major morphological changes were observed in MPEC, including the deformation of pellets, variation of their size, appearance of hyphae and clumps as well as the aggregation of mycelial objects. The presence of talc microparticles also influenced the levels of the studied secondary metabolites produced by S. rimosus. Comparing control and MPEC runs, the addition of talc microparticles increased the amounts of oxytetracycline (9-fold), 2-acetyl-2-decarboxamido-oxytetracycline (7-fold), milbemycin A3+4[O] (3-fold) and CE 108 (1.5-fold), while rimocidin (27-ethyl) and milbemycin β11+4[O] production was reduced. In summary, the addition of talc microparticles to S. rimosus cultivations led to the development of smaller morphological forms like hyphae and clumps as well as to the changes in the amounts of secondary metabolites.
Graphical abstract
miR-17-92a-1 cluster host gene: a key regulator in colorectal cancer development and progression
Authors: Mohajeri Khorasani Amirhossein , Mohammadi Samane , Raghibi Alireza , Haj Mohammad Hassani Behzad , Bazghandi Behina , Mousavi Pegah 
Published: April 2024

AbstractColorectal cancer (CRC), recognized among the five most prevalent malignancies and most deadly cancers, manifests multifactorial influences stemming from environmental exposures, dietary patterns, age, and genetic predisposition. Although substantial progress has been made in comprehending the etiology of CRC, the precise genetic components driving its pathogenesis remain incompletely elucidated. Within the expansive repertoire of non-coding RNAs, particular focus has centered on the miR-17-92a-1 cluster host gene (MIR17HG) and its associated miRNAs, which actively participate in diverse cellular processes and frequently exhibit heightened expression in various solid tumors, notably CRC. Therefore, the primary objective of this research is to undertake an extensive inquiry into the regulatory mechanisms, structural features, functional attributes, and potential diagnostic and therapeutic implications associated with this cluster in CRC. Furthermore, the intricate interplay between this cluster and the development and progression of CRC will be explored. Our findings underscore the upregulation of the miR-17-92a-1 cluster host gene (MIR17HG) and its associated miRNAs in CRC compared to normal tissues, thus implying their profound involvement in the progression of CRC. Collectively, these molecules are implicated in critical oncogenic processes, encompassing metastatic activity, regulation of apoptotic pathways, cellular proliferation, and drug resistance. Consequently, these findings shed illuminating insights into the potential of MIR17HG and its associated miRNAs as promising targets for therapeutic interventions in the management of CRC.
Graphical abstract
A novel diagnostic model based on lncRNA PTPRE expression, neutrophil count and red blood cell distribution width for diagnosis of seronegative rheumatoid arthritis
Authors: Xia Jinfang , Gao Huali , Tang Jifeng , Jiang Renquan , Xiao Lianbo , Sheng Huiming , Lin Jinpiao 
Published: April 2024

AbstractDiagnosis of seronegative rheumatoid arthritis (SNRA) is difficult due to the lack of diagnostic markers. The study aims to construct a novel diagnostic model based on long noncoding RNAs (lncRNAs) expression and laboratory indicators to provide a new idea for diagnostic methods of SNRA. Differentially expressed lncRNAs in peripheral blood cells of RA patients were screened through eukaryotic long noncoding RNA sequencing and validated by quantitative real-time PCR. Meanwhile, the correlation between lncRNAs expression and laboratory indicators was analyzed. The diagnostic value was evaluated by receiver operating characteristic curve analysis. Finally, combined with laboratory indicators, a diagnostic model for SNRA was constructed based on logistic regression and visualized by nomogram. Expression of ADGRE5, FAM157A, PTPN6 and PTPRE in peripheral blood was significantly increased in RA than healthy donors. Meanwhile, we analyzed the relationship between lncRNAs and erythrocyte sedimentation rate, C-reactive protein and CD4 + T cell-related cytokines and transcription factors. Results showed that FAM157A and PTPN6 were positively related to RORγt, and negatively related to GATA3. Moreover, PTPRE has potential discrimination ability between SNRA and healthy donor (AUC = 0.6709). Finally, we constructed a diagnostic model based on PTPRE, neutrophil count and red blood cell distribution width (RDW). The AUC of the model was 0.939 and well-fitted calibration curves. Decision curve analysis indicated the model had better predict performance in SNRA diagnosis. Our study constructed a novel diagnostic model based on PTPRE, neutrophil count and RDW which may serve as a potential tool for the diagnosis of SNRA.
The “bubbles”-study: validation of ultra-short scales for the assessment of positive mental health, life satisfaction, and perceived social support
Authors: Brailovskaia Julia , Teismann Tobias , Precht Lena-Marie , Schneider Silvia , Margraf Jürgen 
Published: April 2024

Abstract
Aim
The positive dimension of mental health should be considered to assess the complete state of a person’s mental health and to protect it. Very brief valid screening instruments implemented in general mental health programs and in therapeutic setting are supportive tools hereby. In the present work, we aimed to develop such tools.

Subject and methods
In two studies (Study 1: N = 1,004; Study 2: N = 1,000) on representative German population samples, we developed and validated three ultra-short scales – the “bubbles” – that consist of only one item per construct based on Positive Mental Health Scale (PMH-Scale; Lukat et al. 2016), Satisfaction with Life Scale (SWLS; Diener et al. 1985), and Social Support Questionnaire (F-SozU K-6; Lin et al. 2019) for the assessment of the positive mental health dimension. The bubbles are designed in a graphical/visual format.

Results
The results of Study 1 and Study 2 revealed that the bubbles are valid instruments that fit the original instruments on the factor level; the bubbles mostly replicated their association pattern with demographic variables, and with variables that belong to the negative and the positive dimension of mental health.

Conclusions
The shortness of the bubbles prevents participants’ fatigue and motivation decrease. Thus, due to their time- and cost-efficiency, the bubbles can be used as brief screening tools in research (e.g., large-scale studies, longitudinal studies, experience sampling paradigms) and in praxis (e.g., mental health programs). Future research should validate the bubbles in clinical context and cross-national.