bluuebunny/crossref_metadata_2025_split huggingface dataset:
Idebenone increases mitochondrial complex I activity in fibroblasts from LHON patients while producing contradictory effects on respiration
Authors: Angebault Claire , Gueguen Naïg , Desquiret-Dumas Valérie , Chevrollier Arnaud , Guillet Virginie , Verny Christophe , Cassereau Julien , Ferre Marc , Milea Dan , Amati-Bonneau Patrizia , Bonneau Dominique , Procaccio Vincent , Reynier Pascal , Loiseau Dominique 
Published: December 2011

Abstract

Background
Leber's hereditary optic neuropathy (LHON) is caused by mutations in the complex I subunits of the respiratory chain. Although patients have been treated with idebenone since 1992, the efficacy of the drug is still a matter of debate.

Methods
We evaluated the effect of idebenone in fibroblasts from LHON patients using enzymatic and polarographic measurements.

Results
Complex I activity was 42% greater in treated fibroblasts compared to controls (p = 0.002). Despite this complex I activity improvement, the effects on mitochondrial respiration were contradictory, leading to impairment in some cases and stimulation in others.

Conclusion
These results indicate that idebenone is able to compensate the complex I deficiency in LHON patient cells with variable effects on respiration, indicating that the patients might not be equally likely to benefit from the treatment.
Photodynamic therapy vs. photochemical internalization: the surgical margin
Authors: Jerjes Waseem , Upile Tahwinder , Radhi Hani , Hopper Colin 
Published: December 2011

Abstract
Controlling tumour margins in head and neck surgery is of the utmost importance in preventing loco-regional spread and distant metastasis, which will ultimately lead to a significant reduction in morbidity and mortality. We comment on the surgical margins in photodynamic therapy and photochemical internalization.
A methodology for converting traditional vertical electrical soundings into 2D resistivity models: Application to the Saïss basin, Morocco
Authors: Riss Joëlle , Luis Fernández-Martínez Juan , Sirieix Colette , Harmouzi Ouassima , Marache Antoine , Essahlaoui Ali 
Published: December 2011

We demonstrated a simple methodology for the reinterpretation of sets of electrical vertical soundings and the production of 2D resistivity models. The method is applied to the northern central part of the subsiding Saïss basin (Morocco) where a series of vertical electrical soundings (VES) were acquired in 1982 and 1984, using the Schlumberger array with current electrodes semispacings between 3 and 3000 m. We describe a methodology used to estimate and sample apparent resistivities collected from traditional single soundings acquired by using a Schlumberger configuration. We then construct a resistivity pseudosection in a format compatible with a Wenner-Schlumberger array to perform 2D electrical tomography. The methodology takes into account the lateral and vertical spatial correlations shown by the apparent resistivities. The tomographic inversions are performed using a commercial inversion software commonly used in industry. Geostatistical tools are used to quantify the spatial correlation between apparent resistivity data. The result is a series of estimated pseudosections that are inverted to produce 2D resistivity models of the basin along geophysical profiles. We focus our attention on one of these profiles (i.e., one 2D resistivity model). The inverted geoelectrical model allows us to propose a geologic model of the basin. Our methodology allows historical and current data carried out using the Schlumberger configuration, to be rapidly reinterpreted in countries where only low-cost methods, such as VES soundings, can be used due to technology and economic constraints. It can also be applied to cases where continuous or pulled-array continuous vertical soundings method, where a tail of electrodes is towed behind a small vehicle while continuously and simultaneously measuring several electrode configurations, is inoperative due to field conditions (survey length, deep layers, and local environmental conditions).
Human Melatonin and Alerting Response to Blue-Enriched Light Depend on a Polymorphism in the Clock Gene PER3
Authors: Chellappa Sarah L. , Viola Antoine U. , Schmidt Christina , Bachmann Valérie , Gabel Virginie , Maire Micheline , Reichert Carolin F. , Valomon Amandine , Götz Thomas , Landolt Hans-Peter , Cajochen Christian 
Published: December 2011

Context:
Light exposure, particularly at the short-wavelength range, triggers several nonvisual responses in humans. However, the extent to which the melatonin-suppressing and alerting effect of light differs among individuals remains unknown.

Objective:
Here we investigated whether blue-enriched polychromatic light impacts differentially on melatonin and subjective and objective alertness in healthy participants genotyped for the PERIOD3 (PER3) variable-number, tandem-repeat polymorphism.

Design, Setting, and Participants:
Eighteen healthy young men homozygous for the PER3 polymorphism (PER35/5and PER34/4) underwent a balanced crossover design during the winter season, with light exposure to compact fluorescent lamps of 40 lux at 6500 K and at 2500 K during 2 h in the evening.

Results:
In comparison to light at 2500 K, blue-enriched light at 6500 K induced a significant suppression of the evening rise in endogenous melatonin levels in PER35/5 individuals but not in PER34/4. Likewise, PER35/5 individuals exhibited a more pronounced alerting response to light at 6500 K than PER34/4 volunteers. Waking electroencephalographic activity in the theta range (5–7 Hz), a putative correlate of sleepiness, was drastically attenuated during light exposure at 6500 K in PER35/5 individuals as compared with PER34/4.

Conclusions:
We provide first evidence that humans homozygous for the PER3 5/5 allele are particularly sensitive to blue-enriched light, as indexed by the suppression of endogenous melatonin and waking theta activity. Light sensitivity in humans may be modulated by a clock gene polymorphism implicated in the sleep-wake regulation.
COLD-PCR Enrichment of Rare Cancer Mutations prior to Targeted Amplicon Resequencing
Authors: Milbury Coren A , Correll Mick , Quackenbush John , Rubio Renee , Makrigiorgos G Mike 
Published: December 2011

Abstract

BACKGROUND
Despite widespread interest in next-generation sequencing (NGS), the adoption of personalized clinical genomics and mutation profiling of cancer specimens is lagging, in part because of technical limitations. Tumors are genetically heterogeneous and often contain normal/stromal cells, features that lead to low-abundance somatic mutations that generate ambiguous results or reside below NGS detection limits, thus hindering the clinical sensitivity/specificity standards of mutation calling. We applied COLD-PCR (coamplification at lower denaturation temperature PCR), a PCR methodology that selectively enriches variants, to improve the detection of unknown mutations before NGS-based amplicon resequencing.

METHODS
We used both COLD-PCR and conventional PCR (for comparison) to amplify serially diluted mutation-containing cell-line DNA diluted into wild-type DNA, as well as DNA from lung adenocarcinoma and colorectal cancer samples. After amplification of TP53 (tumor protein p53), KRAS (v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog), IDH1 [isocitrate dehydrogenase 1 (NADP+), soluble], and EGFR (epidermal growth factor receptor) gene regions, PCR products were pooled for library preparation, bar-coded, and sequenced on the Illumina HiSeq 2000.

RESULTS
In agreement with recent findings, sequencing errors by conventional targeted-amplicon approaches dictated a mutation-detection limit of approximately 1%–2%. Conversely, COLD-PCR amplicons enriched mutations above the error-related noise, enabling reliable identification of mutation abundances of approximately 0.04%. Sequencing depth was not a large factor in the identification of COLD-PCR–enriched mutations. For the clinical samples, several missense mutations were not called with conventional amplicons, yet they were clearly detectable with COLD-PCR amplicons. Tumor heterogeneity for the TP53 gene was apparent.

CONCLUSIONS
As cancer care shifts toward personalized intervention based on each patient's unique genetic abnormalities and tumor genome, we anticipate that COLD-PCR combined with NGS will elucidate the role of mutations in tumor progression, enabling NGS-based analysis of diverse clinical specimens within clinical practice.
Standard Testing Methods for Mobile Bearing Knees
Authors: Walker Peter S. , Haider Hani 
Published: December 2011

Abstract
From 1970 to 2000, many types of total knee arthroplasties were introduced, including mobile bearing knees. In order to mechanically evaluate the designs, tests were developed for factors such as strength, laxity and constraint, kinematics, and wear. A number of the testing methods became ASTM or ISO standards, which are generally required for market introduction. In the year 2000, attention was given to testing methods specifically for mobile bearing knees, because of their mechanical differences from the more widely used fixed-bearing designs. The process began with the identification of potential problems unique to the mobile bearings themselves. These problems included bearing dislocation and deformation of the bearings due to stops and pivots. Wear testing methodology was also seen as important because of the original claim that mobile bearings would have reduced wear compared with fixed bearings due to the lower contact stresses. In this paper, the theoretical framework for defining the tests was specified. Specific tests were then defined for the following potential failure modes: dislocation (‘spin-out’), dynamic dissociation (‘spit-out’), deformation (due to stops, etc.), and endurance and deformation (due to overhang). Existing tests for total knee arthroplasty, in general, were also cited: cyclic fatigue of tibial trays, constraint (upper bearing surface), and wear (with a recommendation for differentiating upper and lower bearing wear). In the future, it is recommended that laboratories and manufacturers use these new tests to gain experience in their use and evaluate the safety of future mobile bearing designs.
Testosterone therapy decreases subcutaneous fat and adiponectin in aging men
Authors: Frederiksen L , Højlund K , Hougaard D M , Mosbech T H , Larsen R , Flyvbjerg A , Frystyk J , Brixen K , Andersen M 
Published: December 2011

ObjectiveTestosterone therapy increases lean body mass and decreases total fat mass in aging men with low normal testosterone levels. The major challenge is, however, to determine whether the metabolic consequences of testosterone therapy are overall positive. We have previously reported that 6-month testosterone therapy did not improve insulin sensitivity. We investigated the effect of testosterone therapy on regional body fat distribution and on the levels of the insulin-sensitizing adipokine, adiponectin, in aging men with low normal bioavailable testosterone levels.DesignA randomized, double-blinded, placebo-controlled study on 6-month testosterone treatment (gel) in 38 men, aged 60–78 years, with bioavailable testosterone <7.3 nmol/l, and a waist circumference >94 cm.MethodsCentral fat mass (CFM) and lower extremity fat mass (LEFM) were measured by dual X-ray absorptiometry. Subcutaneous abdominal adipose tissue (SAT), visceral adipose tissue (VAT), and thigh subcutaneous fat area (TFA) were measured by magnetic resonance imaging. Adiponectin levels were measured using an in-house immunofluorometric assay. Coefficients (b) represent the placebo-controlled mean effect of intervention.ResultsLEFM was decreased (b=−0.47 kg,P=0.07) while CFM did not change significantly (b=−0.66 kg,P=0.10) during testosterone therapy. SAT (b=−3.0%,P=0.018) and TFA (b=−3.0%,P<0.001) decreased, while VAT (b=1.0%,P=0.54) remained unchanged. Adiponectin levels decreased during testosterone therapy (b=−1.3 mg/l,P=0.001).ConclusionTestosterone therapy decreased subcutaneous fat on the abdomen and lower extremities, but visceral fat was unchanged. Moreover, adiponectin levels were significantly decreased during testosterone therapy.
Decrease in circulating fibroblast growth factor 21 after an oral fat load is related to postprandial triglyceride-rich lipoproteins and liver fat
Authors: Matikainen Niina , Taskinen Marja-Riitta , Stennabb Sanna , Lundbom Nina , Hakkarainen Antti , Vaaralahti Kirsi , Raivio Taneli 
Published: December 2011

BackgroundElevated levels of circulating fibroblast growth factor 21 (FGF21) are commonly encountered in type 2 diabetes, dyslipidemia, and non-alcoholic fatty liver disease, all of which share exaggerated postprandial lipemia as a common proatherogenic feature. How FGF21 responds to an oral fat load in man is unknown.MethodsWe measured liver fat contents and subcutaneous and visceral fat volumes in 47 healthy subjects, who also underwent an oral fat load with measurements of plasma FGF21 and free fatty acid (FFA). Triglyceride (TG), apolipoprotein B-48 (apoB-48), and apoB-100 concentrations were measured in triglyceride-rich lipoprotein (TRL) fractions.ResultsWhen compared with fasting levels, the concentration of FGF21 decreased significantly at 4 h (P<0.05) and tended to return to fasting levels at 8 h after an oral fat load. Fasting and postprandial FGF21 correlated significantly with liver fat as well as with TRLs in the chylomicron and especially in very low-density lipoprotein 1 (VLDL1) and VLDL2 fractions representing remnant particles, but not with FFA. Subjects with increased liver fat (>5%,n=12) showed impaired suppression of FGF21 at 4 h (P<0.05) and at 8 h (P=0.01) and demonstrated higher postprandial TG area under the curve in plasma and TRL fractions (P≤0.032) compared with those with normal liver fat (≤5%,n=35).ConclusionsWe observed a significant decrease of FGF21 concentration after an oral fat load. Fasting and postprandial FGF21 levels were closely related to large VLDL and remnants, but not to plasma FFA. Our pilot findings suggest that the postprandial accumulation of TRL remnants and liver fat may modulate postprandial FGF21 levels.
Periodontal Disease is Not Associated With Preeclampsia in Canadian Pregnant Women
Authors: Taghzouti Nawel , Xiong Xu , Gornitsky Mervyn , Chandad Fatiha , Voyer René , Gagnon Guy , Leduc Line , Xu Hairong , Tulandi Togas , Wei Bin , Sénécal Julie , Velly Ana M. , Salah Mohammad H. , Fraser William D. 
Published: December 2011

Background: The findings from the studies on the relationship between periodontal disease and preeclampsia are inconsistent. The objective of this study is to examine the relationship between periodontal disease and preeclampsia.Methods: A multicenter case‐control study was conducted in Quebec, Canada. Preeclampsia was defined as blood pressure ≥140/90 mm Hg and ≥1+ proteinuria after 20 weeks of gestation. Periodontitis was defined as the presence of ≥4 sites with a probing depth ≥5 mm and a clinical attachment loss ≥3 mm at the same sites.Results: A total of 92 preeclamptic women and 245 controls were analyzed. The percentage of periodontal disease was 18.5% in preeclamptic women and 19.2% in normotensive women (crude odds ratio [OR] = 0.96, 95% confidence interval [CI] = 0.52 to 1.77). After adjusting for confounding variables, periodontitis remained not associated with preeclampsia (adjusted OR = 1.13, 95% CI = 0.59 to 2.17).Conclusion: This study does not support the hypothesis of an association between periodontal disease and preeclampsia.
Who Needs What? A Case Study of Post‐disaster Damage and Needs Assessment (DANA) in Vietnam
Authors: Hughey Erin , Bell Heather , Chatman Michael 
Published: December 2011

AbstractDisaster damages and losses cannot be wholly eliminated, and it is essential that communities develop strategies to effectively respond when disaster strikes. Accurate accounting of damage and needs following a disaster can help responders to match existing needs with available resources and assets. The goal of this study was to determine the accuracy and completeness of existing damage and needs assessment (DANA) procedures in Vietnam. Evaluations were made based on a review of available DANA records at the central and provincial government levels, as well as on information collected through semi‐structured interviews with provincial representatives. Provincial level data were collected in five study provinces: Danang, Quang Binh, Quang Nam, Quang Tri, and Thua Thien‐Hue. Results indicate inconsistent DANA templates, procedures, and application. Recommendations include 1) development of a consistent DANA policy; 2) adoption of a standardized methodology for DANA data collection and reporting; and 3) development and implementation of standardized DANA training.
The Social Construction of Research Advice: The American Accounting Association Plays Miss Lonelyhearts
Authors: Fogarty Timothy J. 
Published: December 2011

ABSTRACT
The control of important outcomes in academic accounting by the faculties of a small set of elite institutions has been well documented through a series of outcomes. Less is known about the processes by which this is sustained over time. This paper considers the advice provided to young scholars as one means whereby the social hierarchy is reproduced. Eight specific mechanisms are analyzed and found to bolster belief in the objective meritocracy of ideas in the discipline. Building upon the faux advice provided by Nathanael West's fictional Miss Lonelyhearts, the paper concludes that the stabilizing point embedded in the system is the necessity of people's acceptance of their own inadequacy.
Leadership in Accounting: The New Face of an Old Profession
Authors: Fogarty Timothy J. , Al-Kazemi Saad A. 
Published: December 2011

ABSTRACT
Recent interest in the accounting profession should translate into an inspection of its leadership. Why certain people are leaders and other people are not is a question that bears upon the chances that the profession can regain its stature in the eyes of the public. Even without crisis, an examination of the portals of power adds to our appreciation for the social organization of the accounting profession. Using Accounting Today's list of the 100 most influential people in the field over the first decade of the 21st century, this paper describes these individuals. In many ways, this array reveals the existence of a profession much different than that broadly appreciated in academic circles.
Eco-Effective Management: An Empirical Link between Firm Value and Corporate Sustainability
Authors: Burnett Royce D. , Skousen Christopher J. , Wright Charlotte J. 
Published: December 2011

ABSTRACT
Achieving corporate sustainability requires the implementation of management practices that create long-term shareholder value by embracing opportunities and managing risks deriving from economic, environmental, and social developments. Corporations that are sustainable create value that, in the long run, exceeds their environmental impact (Figge and Hahn 2004). We examine the extent to which investors incrementally value the long-run benefits accruing from adoption of eco-effective management. We posit that adoption of eco-effective management results in increases in firms' market valuation, and that those increases persist beyond the current accounting period. Our results support this hypothesis. This study has broad public policy implications as accountants, managers, and government policymakers shift their focus toward sustainability and rely on market-based mechanisms to further environmental goals.
Data Availability: All data are available from public sources.
Developing Collective Intentionality and Writing the Rules of the Game for Environmental Reporting: A Content Analysis of SOP 96-1 Comment Letters
Authors: Mobus Janet Luft 
Published: December 2011

ABSTRACT
Financial reporting operates in a changing social landscape. One focus of change in the contemporary social landscape is growing recognition of the impact of human activity on the natural environment, a significant part of which derives from economic activity. Evidence exists that financial statement users want environmental accounting information, yet it remains underdeveloped. This underdevelopment becomes notable because it is increasingly clear that long-term economic viability is wholly dependent on ecological sustainability. This study furthers our understanding of financial reporting, accounting standards, and the standard-setting process as institutional practices in the U.S. by exploring whether voices raising these emerging concerns find receptivity in accounting standards setting. Searle's (1995, 1998) work on constructing institutional reality provides the theoretical frame. Results add a layer of empirical analysis to prior accounting research using this frame to critique the openness of discourse in developing financial reporting.
The American Institute of Certified Public Accountants (AICPA) received 77 comment letters in consideration of SOP 96-1, “Environmental Remediation Liabilities.” These letters are evaluated for rhetorical tone using quantitative content analysis. Sixteen letters take issue with the narrow scope of the proposed treatment of environmental reporting. These 16 are further analyzed using qualitative content analysis. Comparing rhetorical tone and meaning content of the comment letters reveals active discussion of important environmental reporting issues that go beyond the narrow technical standard adopted.
Data Availability: Data used in this study are available by contacting the author.
Near Normalization of Metabolic and Functional Features of the Central Nervous System in Type 1 Diabetic Patients With End-Stage Renal Disease After Kidney-Pancreas Transplantation
Authors: Fiorina Paolo , Vezzulli Paolo , Bassi Roberto , Gremizzi Chiara , Falautano Monica , D’Addio Francesca , Vergani Andrea , Chabtini Lola , Altamura Erica , Mello Alessandra , Caldara Rossana , Scavini Marina , Magnani Giuseppe , Falini Andrea , Secchi Antonio 
Published: December 2011

OBJECTIVE
The pathogenesis of brain disorders in type 1 diabetes (T1D) is multifactorial and involves the adverse effects of chronic hyperglycemia and of recurrent hypoglycemia. Kidney-pancreas (KP), but not kidney alone (KD), transplantation is associated with sustained normoglycemia, improvement in quality of life, and reduction of morbidity/mortality in diabetic patients with end-stage renal disease (ESRD).

RESEARCH DESIGN AND METHODS
The aim of our study was to evaluate with magnetic resonance imaging and nuclear magnetic resonance spectroscopy (1H MRS) the cerebral morphology and metabolism of 15 ESRD plus T1D patients, 23 patients with ESRD plus T1D after KD (n = 9) and KP (n = 14) transplantation, and 8 age-matched control subjects.

RESULTS
Magnetic resonance imaging showed a higher prevalence of cerebrovascular disease in ESRD plus T1D patients (53% [95% CI 36–69]) compared with healthy subjects (25% [3–6], P = 0.04). Brain 1H MRS showed lower levels of N-acetyl aspartate (NAA)-to-choline ratio in ESRD plus T1D, KD, and KP patients compared with control subjects (control subjects vs. all, P &lt; 0.05) and of NAA-to-creatine ratio in ESRD plus T1D compared with KP and control subjects (ESRD plus T1D vs. control and KP subjects, P ≤ 0.01). The evaluation of the most common scores of psychological and neuropsychological function showed a generally better intellectual profile in control and KP subjects compared with ESRD plus T1D and KD patients.

CONCLUSIONS
Diabetes and ESRD are associated with a precocious form of brain impairment, chronic cerebrovascular disease, and cognitive decline. In KP-transplanted patients, most of these features appeared to be near normalized after a 5-year follow-up period of sustained normoglycemia.