bluuebunny/crossref_metadata_2025_split huggingface dataset:
Upregulation of microRNA-125b contributes to leukemogenesis and increases drug resistance in pediatric acute promyelocytic leukemia
Authors: Zhang Hua , Luo Xue-Qun , Feng Dan-Dan , Zhang Xing-Ju , Wu Jun , Zheng Yu-Sheng , Chen Xiao , Xu Ling , Chen Yue-Qin 
Published: September 2011

Abstract

Background
Although current chemotherapy regimens have remarkably improved the cure rate of pediatric acute promyelocytic leukemia (APL) over the past decade, more than 20% of patients still die of the disease, and the 5-year cumulative incidence of relapse is 17%. The precise gene pathways that exert critical control over the determination of cell lineage fate during the development of pediatric APL remain unclear.

Methods
In this study, we analyzed miR-125b expression in 169 pediatric acute myelogenous leukemia (AML) samples including 76 APL samples before therapy and 38 APL samples after therapy. The effects of enforced expression of miR-125b were evaluated in leukemic cell and drug-resistant cell lines.

Results

miR-125b is highly expressed in pediatric APL compared with other subtypes of AML and is correlated with treatment response, as well as relapse of pediatric APL. Our results further demonstrated that miR-125b could promote leukemic cell proliferation and inhibit cell apoptosis by regulating the expression of tumor suppressor BCL2-antagonist/killer 1 (Bak1). Remarkably, miR-125b was also found to be up-regulated in leukemic drug-resistant cells, and transfection of a miR-125b duplex into AML cells can increase their resistance to therapeutic drugs,

Conclusions
These findings strongly indicate that miR-125b plays an important role in the development of pediatric APL at least partially mediated by repressing BAK1 protein expression and could be a potential therapeutic target for treating pediatric APL failure.
Use of an amplatzer vascular plug in embolization of a pulmonary artery aneurysm in a case of hughes-stovin syndrome: a case report
Authors: Tzilalis Vasileios D , Vourliotakis Georgios , Tsironis Ioannis A , Tsiligiris Vasileios D , Brountzos Elias N 
Published: September 2011

Abstract

Introduction
Hughes-Stovin syndrome is a rare condition characterized by peripheral deep venous thrombosis accompanied by single or multiple pulmonary arterial aneurysms. The limited number of cases has precluded controlled studies of the management of pulmonary artery aneurysms, which usually cause massive hemoptysis leading to death. This is the first report of a new endovascular treatment of a single large pulmonary arterial aneurysm.

Case presentation
An 18-year-old Caucasian man was referred to our department with recurrent severe hemoptysis. His medical history included Hughes-Stovin syndrome diagnosed during a recent hospital admission. The patient was initially treated with corticosteroids. Because of his recurrent hemoptysis, we decided to embolize a 3.5 cm pulmonary arterial aneurysm using an Amplatzer Vascular Plug. The procedure was not complicated, and the patient's post-intervention course was uneventful. The patient has remained free from any complications of the embolization 36 months after the procedure.

Conclusion
Percutaneous embolization of a single large pulmonary artery aneurysm with an Amplatzer Vascular Plug in a patient with Hughes-Stovin syndrome is a less invasive procedure that represents the best multidisciplinary approach in treating these patients.
CD23+/CD21hi B-cell translocation and ipsilateral lymph node collapse is associated with asymmetric arthritic flare in TNF-Tg mice
Authors: Li Jie , Zhou Quan , Wood Ronald W , Kuzin Igor , Bottaro Andrea , Ritchlin Christopher T , Xing Lianping , Schwarz Edward M 
Published: September 2011

Abstract
Introduction
Rheumatoid arthritis (RA) is a chronic autoimmune disease with episodic flares in affected joints. However, how arthritic flare occurs only in select joints during a systemic autoimmune disease remains an enigma. To better understand these observations, we developed longitudinal imaging outcomes of synovitis and lymphatic flow in mouse models of RA, and identified that asymmetric knee flare is associated with ipsilateral popliteal lymph node (PLN) collapse and the translocation of CD23+/CD21hi B-cells (B-in) into the paracortical sinus space of the node. In order to understand the relationship between this B-in translocation and lymph drainage from flaring joints, we tested the hypothesis that asymmetric tumor necrosis factor (TNF)-induced knee arthritis is associated with ipsilateral PLN and iliac lymph node (ILN) collapse, B-in translocation, and decreased afferent lymphatic flow.

Methods
TNF transgenic (Tg) mice with asymmetric knee arthritis were identified by contrast-enhanced (CE) magnetic resonance imaging (MRI), and PLN were phenotyped as "expanding" or "collapsed" using LNcap threshold = 30 (Arbitrary Unit (AU)). Inflammatory-erosive arthritis was confirmed by histology. Afferent lymphatic flow to PLN and ILN was quantified by near infrared imaging of injected indocyanine green (NIR-ICG). The B-in population in PLN and ILN was assessed by immunohistochemistry (IHC) and flow cytometry. Linear regression analyses of ipsilateral knee synovial volume and afferent lymphatic flow to PLN and ILN were performed.

Results
Afferent lymph flow to collapsed nodes was significantly lower (P < 0.05) than flow to expanding nodes by NIR-ICG imaging, and this occurred ipsilaterally. While both collapsed and expanding PLN and ILN had a significant increase (P < 0.05) of B-in compared to wild type (WT) and pre-arthritic TNF-Tg nodes, B-in of expanding lymph nodes (LN) resided in follicular areas while B-in of collapsed LN were present within LYVE-1+ lymphatic vessels. A significant correlation (P < 0.002) was noted in afferent lymphatic flow between ipsilateral PLN and ILN during knee synovitis.

Conclusions
Asymmetric knee arthritis in TNF-Tg mice occurs simultaneously with ipsilateral PLN and ILN collapse. This is likely due to translocation of the expanded B-in population to the lumen of the lymphatic vessels, resulting in a dramatic decrease in afferent lymphatic flow. PLN collapse phenotype can serve as a new biomarker of knee flare.
The association between systemic glucocorticoid therapy and the risk of infection in patients with rheumatoid arthritis: systematic review and meta-analyses
Authors: Dixon William G , Suissa Samy , Hudson Marie 
Published: September 2011

Abstract
Introduction
Infection is a major cause of morbidity and mortality in patients with rheumatoid arthritis (RA). The objective of this study was to perform a systematic review and meta-analysis of the effect of glucocorticoid (GC) therapy on the risk of infection in patients with RA.

Methods
A systematic review was conducted by using MEDLINE, EMBASE, CINAHL, and the Cochrane Central Register of Controlled Trials database to January 2010 to identify studies among populations of patients with RA that reported a comparison of infection incidence between patients treated with GC therapy and patients not exposed to GC therapy.

Results
In total, 21 randomised controlled trials (RCTs) and 42 observational studies were included. In the RCTs, GC therapy was not associated with a risk of infection (relative risk (RR), 0.97 (95% CI, 0.69, 1.36)). Small numbers of events in the RCTs meant that a clinically important increased or decreased risk could not be ruled out. The observational studies generated a RR of 1.67 (1.49, 1.87), although significant heterogeneity was present. The increased risk (and heterogeneity) persisted when analyses were stratified by varying definitions of exposure, outcome, and adjustment for confounders. A positive dose-response effect was seen.

Conclusions
Whereas observational studies suggested an increased risk of infection with GC therapy, RCTs suggested no increased risk. Inconsistent reporting of safety outcomes in the RCTs, as well as marked heterogeneity, probable residual confounding, and publication bias in the observational studies, limits the opportunity for a definitive conclusion. Clinicians should remain vigilant for infection in patients with RA treated with GC therapy.
Constitutive expression of cathepsin K in the human intervertebral disc: new insight into disc extracellular matrix remodeling via cathepsin K and receptor activator of nuclear factor-κB ligand
Authors: Gruber Helen E , Ingram Jane A , Hoelscher Gretchen L , Zinchenko Natalia , Norton H James , Hanley Edward N 
Published: September 2011

Abstract
Introduction
Cathepsin K is a recently discovered cysteine protease which cleaves the triple helical domains of type I to II collagen. It has been shown to be up-regulated in synovial tissue from osteoarthritic and rheumatoid patients, and is a component in normal and nonarthritic cartilage, where it increases with aging. Studies on heart valve development have recently shown that receptor activator of nuclear factor-κB ligand (RANKL) acts during valve remodeling to promote cathepsin K expression. Since extracellular matrix remodeling is a critical component of disc structure and biomechanical function, we hypothesized that cathepsin K and RANKL may be present in the human intervertebral disc.

Methods
Studies were performed following approval of the authors' Human Subjects Institutional Review Board. Six annulus specimens from healthier Thompson grade I to II discs, and 12 specimens from more degenerate grade III to IV discs were utilized in microarray analysis of RANKL and cathepsin K gene expression. Immunohistochemistry was also performed on 15 additional disc specimens to assess the presence of RANKL and cathepsin K.

Results
Cathepsin K gene expression was significantly greater in more degenerated grade III to IV discs compared to healthier grade I to II discs (P = 0.001). RANKL was also identified with immunohistochemistry and molecular analyses. RANKL gene expression was also significantly greater in more degenerated discs compared to healthier ones (P = 0.0001). A significant linear positive correlation was identified between expression of cathepsin K and RANKL (r2 = 92.2; P < 0.0001).

Conclusions
Extracellular matrix remodeling is a key element of disc biology. Our use of an appropriate antibody and gene expression studies showed that cathepsin K is indeed present in the human intervertebral disc. Immunolocalization and molecular analyses also confirmed that RANKL is present in the human disc. Expression of RANKL was found to be significantly greater in more degenerated compared to healthier discs (P = 0.0001). Cathepsin K gene expression levels showed a positive, significant correlation with RANKL expression. Based on these data, we propose that cathepsin K plays a significant role in disc matrix remodeling and in matrix degradation in the proinflammatory cytokine-rich microenvironment of the degenerating disc.
NEGATIVE INDUCED‐POLARIZATION EFFECTS FROM LAYERED MEDIA
Authors: Nabighian Misac N. , Elliot Charles L. 
Published: September 2011

Negative induced‐polarization responses from lateral resistivity distributions such as near‐surface vertical contacts and dikes, three‐dimensional buried bodies, etc., have been well known for years. Recent comprehensive computations of resistivity and induced‐polarization response functions over a three horizontally layered earth have revealed that negative induced‐polarization effects can also occur whenever the geoelectric section is of K type (ρ1, < ρ2 > ρ3) or Q type (ρ1 > ρ2 > ρ3). For such sections, the induced‐polarization function of the first layer (as defined by Seigel) can be negative. As a result, if the first layer is polarizable the response from deeper layers can be severely masked solely as a function of the resistivity distribution, thus limiting the depth of detection of buried sources. It is shown that for an m‐layered earth, negative induced‐polarization functions can be expected for those layers lying above a K or Q type sequence in the resistivity section. Any application of induced‐polarization surveys must thus be preceded by a thorough evaluation of the resistivity data. The same applies for the quantitative interpretation of IP data.
Hepatitis C: a patient's journey from a psychiatrist's perspective
Authors: Badrakalimuthu Vellingiri Raja , Rumball Daphne , Chawla Amit 
Published: September 2011

SummaryThere is an increasing prevalence of hepatitis C, particularly among intravenous drug users. Estimates of infection with the hepatitis C virus (HCV) in patients with severe mental illness in the USA have ranged from 8.5% to 19.6%. Alcohol use and misuse is the single biggest contributory factor to the development of fatal liver disease in people with hepatitis C. Neuropsychiatric symptoms are present in HCV infection, independent of any treatment. Despite such alarming observations, the success rate of antiviral treatment in chronic hepatitis C has improved considerably in recent years. This article explores psychiatrists' involvement in advocating for treatment inclusion, assessing patients' capacity to make treatment-related decisions and supporting the medical team in managing the complex journey a patient undertakes from being at high risk to receiving treatment for neuropsychiatric symptoms.
The practice of evidence-based psychiatry today
Authors: Wallace John 
Published: September 2011

SummaryResearch that should change clinical practice is often ignored for years. Fifty-five new trials are reported in medicine every day. Psychiatrists need to have some way to sift, digest and act on new research to benefit their patients. This article outlines the key elements of evidence-based psychiatry in an attempt to address these needs. Initially, an evidence-based approach is about asking an answerable question, searching for the evidence and then critically appraising the available information for its validity and relevance. The fourth step involves integrating the evidence with clinical expertise and the patient's values. The whole idea is to improve real-life patient care. This article outlines a number of the tools needed to overcome some of the common problems that psychiatrists face when trying to find and use published research results to help solve patients' problems.
Combining routine outcomes measurement and ‘Payment by Results’:
will it work and is it worth it?
Authors: Macdonald Alastair J. D. , Elphick Martin 
Published: September 2011

SummaryThe Department of Health in England has long encouraged the routine
measurement of clinical outcomes in mental health services but has now
decided to use outcome measures as part of a new payments system – Payment
by Results. We examine how these two policies should or might interact.
Oberflächenrekonstruktion mittels Stereodeflektometrie
Authors: Werling Stefan , Beyerer Jürgen 
Published: September 2011

Zusammenfassung
Stereoverfahren zur Inspektion und Rekonstruktion spiegelnder Oberflächen können global versagen. Hierzu wird ein konstruktives Verfahren zur Erzeugung von nicht unterscheidbaren Flächenpaaren vorgestellt. Es ergeben sich daraus Bedingungen für die Anwendung von Stereoverfahren zur Rekonstruktion spiegelnder Objekte. Die Rekonstruktion komplex geformter Objekte basiert in der Regel auf vielen einzelnen Teilmessungen. Überlappen sich diese Messbereiche, so kann dort das deflektometrische Stereo zur Regularisierung des Rekonstruktionsproblems gewinnbringend eingesetzt werden.
Payment for Obesity Services: Examples and Recommendations for Stage 3 Comprehensive Multidisciplinary Intervention Programs for Children and Adolescents
Authors: Slusser Wendy , Staten Karan , Stephens Karen , Liu Lenna , Yeh Christine , Armstrong Sarah , DeUgarte Daniel A. , Haemer Matthew 
Published: September 2011

OBJECTIVE:
The Reimbursement and Payment Subcommittee of the National Association of Children's Hospitals and Related Institutions FOCUS on a Fitter Future group sought to guide medical providers, patients, and payers to better serve obese children and adolescents to enable optimum health. Recommendations are provided for the essential components of a stage 3 comprehensive multidisciplinary intervention program as defined by the 2007 Expert Committee recommendations. In addition, suggestions are offered for a stepwise approach to implement these recommendations.

METHODS:
In 2009, key informant interviews were conducted with 15 children's hospitals participating in FOCUS on a Fitter Future and 1 nonparticipating hospital. Interview transcripts identified 5 financially sustainable stage 3 programs, each funded differently.

RESULTS:
The stage 3 programs interviewed ranged from being nascent to 21 years old (27%, &lt;2 years; 47%, 2–6 years; 27%, &gt;6 years). All of them had multidisciplinary teams that delivered services through 1 of 3 institutional structures: 60% freestanding; 7% specialty; and 33% hospital within a hospital. One-third of them had 1 to 2 funding sources, and 67% had ≥3 sources.

CONCLUSIONS:
The stage 3 programs in this review shared some common strategies for achieving financial stability. All of them followed key strategies of the chronic care model, the details of which led to the following recommendation: stage 3 programs should include a health care team with a medical provider, registered dietitian, physical activity specialist, mental health specialist, and coordinator who, as a team, provide service to overweight and obese children at no less than moderate intensity (26–75 hours).
Differing Success of Defense Strategies in Two Parasitoid Wasps in Protecting their Pupae Against a Secondary Hyperparasitoid
Authors: Harvey Jeffrey A , Gols Rieta , Tanaka Toshiharu 
Published: September 2011

Abstract
During their larval development, endoparasitoids are known to dispose of host resources in several different ways. Some parasitoid wasps consume most or all tissues of the host, whereas others consume a small fraction of host resources and either ensure that the host moves away from the pupation site or allow the host to remain close to the parasitoid cocoon(s). Using a single host species, Mythimna separata Walker (Lepidoptera: Noctuidae), this study compares the success of the two pupation strategies in the solitary parasitoids Microplitis sp. and Meteorus pulchricornis Wesmael (Hymenoptera: Braconidae) against attack from a secondary hyperparasitoid, Gelis agilis F. (Hymenoptera: Ichneumonidae). The caudal appendages of M. separata caterpillars parasitized by Microplitis sp. remain physically attached to parasitoid cocoons and the caterpillars behave aggressively when disturbed. However, after Me. pulchricornis larvae emerge from caterpillars of their host, M. separata, the parasitoid larvae pupate in cocoons that are suspended by a single thick thread that hangs 1–2 cm from under a leaf. In choice tests conducted in petri dishes, significantly fewer cocoons of Microplitis sp. attended by caterpillars than unattended cocoons were hyperparasitized by G. agilis. By contrast, Me. pulchricornis cocoons that were hanging from corn, Zea mays L., plants were hyperparasitized as frequently as those which were attached to leaves. We discuss the potentially different selection pressures generated among natural enemies such as predators and hyperparasitoids in determining optimal pupal defense strategies in primary parasitoids.
Context-Dependent Stridulatory Responses ofLeptogenys kitteli(Hymenoptera: Formicidae) to Social, Prey, and Disturbance Stimuli
Authors: Chiu Y-K , Mankin R W , Lin C-C 
Published: September 2011

AbstractIndividual Leptogenys kitteli (Hymenoptera: Formicidae) workers produce stridulatory pulses at faster rates after exposure to prey larvae placed nearby the nest and after air-puff disturbances at the nest entrance than during unstimulated social interactions within the nest. Workers produce chirps (trains of stridulatory pulses) at faster rates after exposure to prey larvae than during unstimulated social interactions, including groups of chirps (bursts) where the intervals between chirps decrease below 60 ms. Such bursts do not occur in unstimulated social interactions. Chirp bursts with intervals &lt;10 ms (disturbance bursts) occur immediately after air puffs at the nest entrance. Disturbance bursts are not observed after exposure to prey larvae or during unstimulated social interactions. The rates of disturbance bursts decline rapidly within 10 s after an air puff, whereas episodes of chirp bursts extend over periods of 30 s or longer when groups of ants are moving prey larvae into the nest. The differences in the rates of stridulatory pulses and chirps and in the durations of stridulatory activity observed in the context of different types, intensities, and durations of stimulation contribute to evidence that stridulation has a significant communicatory role in colony activities of many ant species, even in genera, such as Leptogenys, in which a stridulatory organ has not been retained in every species. A better understanding of how ants produce and interpret vibrations may lead to new methods that to improve attractiveness of baits, or repel ants from electrical equipment housings where opportunistic colonies frequently nest.
Accuracy of the use of triaxial accelerometry for measuring daily activity as a predictor of daily maintenance energy requirement in healthy adult Labrador Retrievers
Authors: Wrigglesworth David J. , Mort Emily S. , Upton Sarah L. , Miller Andrew T. 
Published: September 2011

Abstract
Objective—To determine accuracy of the use of triaxial accelerometry for measuring daily activity as a predictor of maintenance energy requirement (MER) in healthy adult Labrador Retrievers.
Animals—10 healthy adult Labrador Retrievers.
Procedures—Dogs wore an accelerometer for two 2-week periods, with data on daily activity successfully collected for 24 to 26 days. These data, along with body weight, were used as independent variables in a multiple linear regression model to predict the dependent variable of daily MER. The predictive accuracy of the model was compared with that of a model that excluded activity. Dietary energy intake at a stated amount of body weight stability was used as an equivalent measure of MER in these analyses.
Results—The multiple linear regression model that included body weight and daily activity as independent variables could be used to predict observed MER with a mean absolute error of 63.5 kcal and an SE of estimation of 94.3 kcal. Removing activity from the model reduced the predictive accuracy to a mean absolute error of 129.8 kcal and an SE of estimation of 165.4 kcal.
Conclusions and Clinical Relevance—Use of triaxial accelerometers to provide an independent variable of daily activity yielded a marked improvement in predictive accuracy of the regression model, compared with that for a model that used only body weight. Improved accuracy in estimations of MER could be made for each dog if an accelerometer was used to record its daily activity.