bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immediate Breast Reconstruction With Latissimus Dorsi Myocutaneous Flap and Silicone Implant Followed by Adjuvant Radiotherapy for Breast Cancer
Authors: D'Alessandro Gabriel Salum , Munhoz Alexandre Mendonça , Takeuchi Fabiana Midori , Povedano Alejandro , Sampaio Goes João Carlos 
Published: May 2024

Background
About 30% to 50% of women with breast cancer undergo mastectomy, and approximately 50% of them will receive adjuvant radiotherapy (ART). This study evaluates the medium- and long-term impact of ART after immediate breast reconstruction (IBR) with latissimus dorsi myocutaneous (LDM) flap and silicone implants.

Methods
Clinical, surgical, and oncological data were retrospectively collected and analyzed based on the medical records of 176 patients who had undergone IBR with LDM flap and silicone implants.

Results
The data showed that 7.4% of patients had a history of previous radiotherapy, 56.3% received ART, 31.8% developed capsular contracture with a mean follow-up of 58.1 months, and 14.2% of surgeries were categorized as procedures with a prolonged operating time, lasting above 1 SD of the observed mean. Those who experienced prolonged operating time (odds ratio, 4.72; 95% confidence interval, 1.72–12.93; P = 0.003) and those who received ART (odds ratio, 7.38; 95% confidence interval, 3.18–17.10; P < 0.001) were more likely to develop capsular contracture. Thirty-two patients (18%) underwent capsulectomy with implant replacement, and 7 patients (4%) had the implant removed. The mean time between IBR and reoperation was 29.1 months. Patients who received ART were 2.84 times more likely to experience reconstruction failure or undergo implant-related reoperation (P = 0.002).

Conclusions
The results indicated that IBR with LDM flap and silicone implant followed by ART is a safe procedure, resulting in low rates of reconstruction failure. However, ART increased the likelihood of capsular contracture development and implant-related reoperation, having a negative effect on reconstructed breasts.
Refinement of Surgery for Postaxial Polysyndactyly of the Toes
Authors: Ishikawa Kosuke , Furukawa Hiroshi , Yamamoto Yuhei , Funayama Emi , Miura Takahiro , Maeda Taku 
Published: May 2024

Purpose
Treatment for polysyndactyly of the toes aims at cosmetic improvement but the lateroplantar rotation of the new fifth toe remains challenging. This study evaluated our novel surgical procedure for postaxial polysyndactyly of the toes.

Materials and Methods
Patients with postaxial polysyndactyly involving the fourth, fifth, and sixth toes treated in 2007 to 2017 with a minimum follow-up duration of 1 year were retrospectively investigated. Our aims of surgery for this condition were to avoid excessive lateroplantar rotation of the new fifth toe by using a proximally elongated plantar “shark-fin flap” and to make the tip of this toe appear to be naturally pointing inward by using the dog-ear component of the flap on the tip of the toe. The excess skin of the shark-fin flap was grafted onto the lateral surface of the fourth toe. Lateroplantar rotation of the fifth toe in these patients was compared with that in photographs of the feet of 96 normal 4-year-old children.

Results
A total of 11 feet in 10 patients (6 male, 4 female; mean age 1.3 years) were analyzed. Syndactyly between the fourth and fifth toes was complete in 3 feet, incomplete at the level of the distal interphalangeal joint of the fifth toe in 5, and incomplete at the level of the proximal interphalangeal joint of the fifth toe in 3. Lateroplantar rotation of the fifth toe, evaluated by the mean angle between 2 intersecting lines extending from the proximal nail fold of the third and fifth toes, was 25 ± 10° in normal feet and 0 ± 12° in operated feet with polysyndactyly. The absolute left-right difference in this angle was 7 ± 5° in normal children and 22 ± 12° in patients with polysyndactyly. Valgus deformity of the new fifth toe improved in all patients during a mean postoperative follow-up of 3.8 years.

Conclusions
Using our procedure, no excessive lateroplantar rotation has been observed when the tip of the fifth toe is inclined inward using a dog-ear flap component. This procedure could be useful in patients in whom the cosmetic outcome is a priority.
Quality-of-Life Outcomes in Oral Cancer Patients Reconstructed With Double Free Flaps
Authors: Dang Rushil R. , Lin Jennifer An-Jou , Hung Shao-Yu , Wei Fu-Chan 
Published: May 2024

Purpose
This study aimed to evaluate quality of life (QOL) in patients with locally advanced oral cancer who underwent surgical resection followed by simultaneous double free flap reconstruction.

Methods
Institutional database was reviewed from 2015 to 2021 and prospectively collected University of Washington Quality of Life data that were extracted for patients who met the inclusion criteria. Mean, composite, and best percentage scores were computed. Wilcoxon signed rank test was used to analyze differences between groups.

Results
Thirteen patients completed the assessment, all of them being male with a mean follow-up of 2.2 ± 1.2 years. Most common double free flap combination was fibula osteoseptocutaneous flap and anterolateral thigh flap (n = 11). Improvement in pain (P = 0.01) domain score with decline in speech (P = 0.01) and taste (P = 0.02) was noted along with an overall decline in QOL (P = 0.001) after cancer diagnosis. A decrease in physical function was seen postoperatively. Chewing and saliva were the most cited postoperative patient-reported domain affecting QOL.

Conclusions
In double free flap reconstructed patients, in the postoperative phase, pain scores improved, whereas speech and taste scores declined. The overall health-related QOL declined compared with before cancer diagnosis.
Association Between Prescription Drug Monitoring Program Use Mandate and Opioid Prescribing and Patient-Reported Outcomes After Surgery
Authors: Chua Kao-Ping , Nguyen Thuy D. , Brummett Chad M. , Bohnert Amy S. , Gunaseelan Vidhya , Englesbe Michael J. , Lee Stephanie , Waljee Jennifer F. 
Published: May 2024

Objective:
To evaluate changes in opioid prescribing and patient-reported outcomes after surgery following implementation of Michigan’s prescription drug monitoring program (PDMP) use mandate in June 2018.

Background:
Most states mandate clinicians to query prescription drug monitoring program (PDMP) databases before prescribing controlled substances. Whether these PDMP use mandates affect opioid prescribing and patient-reported outcomes after surgery is unclear, especially among patients with elevated “Narx” scores, a risk score for overdose death used in most PDMPs.

Methods:
We conducted an interrupted time series analysis of a statewide surgical registry linked to Michigan’s PDMP database. Analyses included adults undergoing general surgical procedures during January 2017-October 2019. Outcomes included monthly mean days supplied in dispensed opioid prescriptions (those filled within 3 days of discharge) and monthly mean scores for 3 patient-reported outcomes (pain in the week after surgery, care satisfaction, regret undergoing surgery). Segmented regression models were used to assess for level and slope changes in outcomes in June 2018. Analyses were repeated among patients with Narx scores ≥200, a threshold that defines the top quartile.

Results:
Analyses included 21,897 patients. The mandate was associated with a -0.5 (95% CI: -0.8, -0.2) level decrease in mean days supplied in dispensed opioid prescriptions, but not with worsened patient-reported outcomes. Findings were similar among patients with Narx scores ≥200.

Conclusions:
Following implementation of Michigan’s PDMP use mandate, the duration of opioid prescriptions decreased, but patient-reported outcomes did not worsen. Findings suggest PDMP use mandates may not be associated with worsened experience among general surgical patients.
Chemo-enzymatic synthesis of bioactive compounds from traditional Chinese medicine and medicinal plants
Authors: Liu Shuang , Yang Shuo , Su Ping 
Published: May 2024

Abstract
The production of bioactive compounds from traditional Chinese medicine and medicinal plants mainly depends on the extraction and separation from medicinal materials, which is time-consuming, laborious, and requires large amounts of medicinal resources. As the market demand for bioactive compounds increases, the shortage of medicinal resources tops the list. For a stable and sustainable supply of affordable bioactive compounds, it is necessary to optimize chemosynthetic and biosynthetic pathways. Although some progress has been made in chemocatalysis and biosynthesis, there are drawbacks and bottlenecks in current approaches. We hold the opinion that the combination of chemosynthesis and biosynthesis will be the key direction to efficiently produce bioactive compounds. Chemo-enzymatic synthesis, a strategy that combines biosynthesis and chemosynthesis, is an alternative approach for the heterologous production of bioactive compounds. This paper reviews the recent advances in the chemo-enzymatic synthesis of bioactive compounds derived from traditional Chinese medicine and medicinal plants, highlights the potential application, and presents our perspectives for future research.
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Authors: Southwell James W , Wilson Keith S , Thomas Gavin H , Duhme-Klair Anne-Kathrin 
Published: May 2024

Iron is an essential nutrient for microbial growth and bacteria have evolved numerous routes to solubilise and scavenge this biometal, which is often present at very low concentrations in host tissue. We recently used a MOPS-based medium to induce iron limitation in Escherichia coli K-12 during the characterisation of novel siderophore conjugated antibiotics. In this study we confirm that growth media derived from commercially-available M9 salts are unsuitable for studies of iron-limited growth, likely through the contamination of the sodium phosphate buffer components with over 100 µM iron. In contrast, MOPS-based media that are treated with metal-binding Chelex® resin, allow the free iron concentration to be reduced to growth-limiting levels. Despite these measures a small amount of E. coli growth is still observed in these iron-depleted media. By growing E. coli in conditions that theoretically increase the demand for iron-dependent enzymes, namely by replacing the glucose carbon source for acetate and by switching to a microaerobic atmosphere, we can reduce background growth even further. Finally, we demonstrate that by adding an exogeneous siderophore to the growth media which is poorly used by E. coli, we can completely prevent growth, perhaps mimicking situation in host tissue. In conclusion, this short study provides practical experimental insight into low iron media and how to augment the growth conditions of E. coli for extreme iron-limited growth.
Analysis of auxin responses in the fernCeratopteris richardiiidentifies tissue ontogeny as a major determinant for response properties
Authors: Woudenberg Sjoerd , Alvarez Melissa Dipp , Rienstra Juriaan , Levitsky Victor , Mironova Victoria , Scarpella Enrico , Kuhn Andre , Weijers Dolf 
Published: May 2024

AbstractThe auxin signalling molecule regulates a range of plant growth and developmental processes. The core transcriptional machinery responsible for auxin-mediated responses is conserved across all land plants. Genetic, physiological and molecular exploration in bryophyte and angiosperm model species have shown both qualitative and quantitative differences in auxin responses. Given the highly divergent ontogeny of the dominant gametophyte (bryophytes) and sporophyte (angiosperms) generations, however, it is unclear whether such differences derive from distinct phylogeny or ontogeny. Here, we address this question by comparing a range of physiological, developmental and molecular responses to auxin in both generations of the model fernCeratopteris richardii. We find that auxin response in Ceratopteris gametophytes closely resembles that of a thalloid bryophyte, whereas the sporophyte mimics auxin response in flowering plants. This resemblance manifests both at phenotypic and transcriptional level. Furthermore, we show that disrupting auxin transport can lead to ectopic sporophyte induction on the gametophyte, suggesting a role for auxin in the alternation of generations. Our study thus identifies ontogeny, rather than phylogeny, as a major determinant of auxin response properties in land plants.Summary statementStudies in angiosperms and bryophytes have left unresolved the roles of tissue ontogeny and species phylogeny in auxin response. We address that problem by characterizing auxin response in a fern.
Live-cell single-molecule fluorescence microscopy for protruding organelles reveals regulatory mechanisms of MYO7A-driven cargo transport in stereocilia of inner ear hair cells
Authors: Miyoshi Takushi , Vishwasrao Harshad D. , Belyantseva Inna A. , Sajeevadathan Mrudhula , Ishibashi Yasuko , Adadey Samuel M. , Harada Narinobu , Shroff Hari , Friedman Thomas B. 
Published: May 2024

AbstractStereocilia are unidirectional F-actin-based cylindrical protrusions on the apical surface of inner ear hair cells and function as biological mechanosensors of sound and acceleration. Development of functional stereocilia requires motor activities of unconventional myosins to transport proteins necessary for elongating the F-actin cores and to assemble the mechanoelectrical transduction (MET) channel complex. However, how each myosin localizes in stereocilia using the energy from ATP hydrolysis is only partially understood. In this study, we develop a methodology for live-cell single-molecule fluorescence microscopy of organelles protruding from the apical surface using a dual-view light-sheet microscope, diSPIM. We demonstrate that MYO7A, a component of the MET machinery, traffics as a dimer in stereocilia. Movements of MYO7A are restricted when scaffolded by the plasma membrane and F-actin as mediated by MYO7A’s interacting partners. Here, we discuss the technical details of our methodology and its future applications including analyses of cargo transportation in various organelles.
Tigers in North Dakota; Tiger Salamander (Ambystoma tigrinum) eDNA Surveying for Presence in Cattle Ponds
Authors: Showalter Evan R 
Published: May 2024

AbstractEnvironmental DNA (eDNA) allows for efficient and convenient data collection using DNA segments to confirm species presence. There is little literature using eDNA to specifically examine tiger salamander presence in North Dakota. I sampled 8 different cattle ponds in Western North Dakota twice each using a set eDNA collection method from the United States Department of Agriculture. After collecting water samples, the filters were sent to Jonah Ventures for DNA extraction and metabarcoding. Samples were incubated, extracted, ran through a first round of Polymerase Chain Reaction (PCR) inspected on agarose gel. Then the amplicons–product of an amplified or replicated piece of DNA, were cleaned, a second round of PCR followed, then the amplicons were cleaned again. Finally sequencing and bioinformatics took place. Salamanders (genusAmbystoma) were detected at 5 of the 8 sites. Average single season Bayesian occupancy point estimate was 0.655 ± (.165), with a detection probability of 0.735± (.156) over the two site visits. Of the covariates including elevation, surface area, and air temperature, air temperature on visit 2 had the best model but still was not significant. There was no significant correlation between any of the covariates and the naïve detections. This method was effective in detecting the genusAmbystoma, however more work and greater sample site variation could elucidate impacts of species level presence absence data.
Scheduled feeding improves sleep in a mouse model of Huntington’s disease
Authors: Chiem Emily , Zhao Kevin , Dell’Angelica Derek , Ghiani Cristina A. , Paul Ketema N. , Colwell Christopher S. 
Published: May 2024

AbstractSleep disturbances are common features of neurodegenerative disorders including Huntington’s disease (HD). The sleep and circadian disruptions are recapitulated in animal models, and these models provide the opportunity to evaluate whether circadian interventions can be effective countermeasures for neurodegenerative disease. Time restricted feeding (TRF) interventions successfully improve activity rhythms, sleep behavior and motor performance in mouse models of HD. Seeking to determine if these benefits of scheduled feeding extend to physiological measures of sleep, electroencephalography (EEG) was used to measure sleep/wake states and polysomnographic patterns in adult mice (six mo-old) under TRF andad libfeeding (ALF). With each diet, both male and female wild-type (WT) and bacterial artificial chromosome transgenic (BACHD) mice were evaluated. Our findings show that male, but not female, BACHD mice exhibited significant changes in the temporal patterning of wake and non-rapid eye movement (NREM) sleep. The TRF intervention reduced the inappropriate early morning activity by increasing NREM sleep in the male BACHD mice. In addition, the scheduled feeding reduced sleep fragmentation (# bouts) in the male BACHD mice. The phase of the rhythm in rapid-eye movement (REM) sleep was significantly altered by the scheduled feeding. The treatment did impact the power spectral curves during the day in male but not female mice. Sleep homeostasis, as measured by the response to six hours of gentle handling, was not altered by the diet. Thus, TRF improves the temporal patterning and fragmentation of NREM sleep without impacting sleep homeostasis. This work adds critical support to the view that sleep is a modifiable risk factor in neurodegenerative diseases.
StereoMM: A Graph Fusion Model for Integrating Spatial Transcriptomic Data and Pathological Images
Authors: Luo Bingying , Teng Fei , Tang Guo , Chen Weixuan , Qu Chi , Liu Xuanzhu , Liu Xin , Liu Xing , Huang Huaqiang , Feng Yu , Zhang Xue , Jian Min , Li Mei , Xi Feng , Li Guibo , Liao Sha , Chen Ao , Xu Xun , Zhang Jiajun 
Published: May 2024

AbstractSpatially resolved omics technologies generating multimodal and high-throughput data lead to the urgent need for advanced analysis to allow the biological discoveries by comprehensively utilizing information from multi-omics data. The H&E image and spatial transcriptomic data indicate abundant features which are different and complementary to each other. AI algorithms can perform nonlinear analysis on these aligned or unaligned complex datasets to decode tumoral heterogeneity for detecting functional domain. However,the interpretability of AI-generated outcomes for human experts is a problem hindering application of multi-modal analysis in clinic. We presented a machine learning based toolchain called StereoMM, which is a graph fusion model that can integrate gene expression, histological images, and spatial location. StereoMM firstly performs information interaction on transcriptomic and imaging features through the attention module, guaranteeing explanations for its decision-making processes. The interactive features are input into the graph autoencoder together with the graph of spatial position, so that multimodal features are fused in a self-supervised manner. Here, StereoMM was subjected to mouse brain tissue, demonstrating its capability to discern fine tissue architecture, while highlighting its advantage in computational speed. Utilizing data from Stereo-seq of human lung adenosquamous carcinoma and 10X Visium of human breast cancer, we showed its superior performance in spatial domain recognition over competing software and its ability to reveal tumor heterogeneity. The fusion approach for imaging and gene expression data within StereoMM aids in the more accurate identification of domains, unveils critical molecular features, and elucidates the connections between different domains, thereby laying the groundwork for downstream analysis.
Time-lapse Image Super-resolution Neural Network with Reliable Confidence Quantification for Optical Microscopy
Authors: Qiao Chang , Liu Shuran , Wang Yuwang , Xu Wencong , Geng Xiaohan , Jiang Tao , Zhang Jingyu , Meng Quan , Qiao Hui , Li Dong , Dai Qionghai 
Published: May 2024

AbstractSingle image super-resolution (SISR) neural networks for optical microscopy have shown great capability to directly transform a low-resolution (LR) image into its super-resolution (SR) counterpart, enabling low-cost long-term live-cell SR imaging. However, when processing time-lapse data, current SISR models failed to exploit the important temporal dependencies between neighbor frames, often resulting in temporally inconsistent outputs. Besides, SISR models are subject to inference uncertainty that is hard to accurately quantify, therefore it is difficult to determine to what extend can we trust the inferred SR images. Here, we first build a large-scale, high-quality fluorescence microscopy dataset for the time-lapse image super-resolution (TISR) task, and conducted a comprehensive evaluation on two essential components of TISR neural networks, i.e., propagation and alignment. Second, we devised a deformable phase-space alignment (DPA) based TISR neural network (DPA-TISR), which adaptively enhances the cross-frame alignment in the phase domain and outperforms existing state-of-the-art SISR and TISR models. Third, we combined the Bayesian training scheme and Monte Carlo dropout with DPA-TISR, developing Bayesian DPA-TISR, and designed an expected calibration error (ECE)minimization framework to obtain a well-calibrated confidence map along with each output SR image, which reliably implicates potential inference errors. We demonstrate the unique characteristics of Bayesian DPA-TISR underlie the ultralong-term live-cell SR imaging capability with high spatial fidelity, superb temporal consistency, and accurate confidence quantification on a wide variety of bioprocesses.