bluuebunny/crossref_metadata_2025_split huggingface dataset:
Individualized Therapy: Role of Thiopurine S-Methyltransferase Protein and Genetic Variants
Authors: Pavlović Sonja , Zukić Branka 
Published: August 2010

Individualized Therapy: Role of Thiopurine S-Methyltransferase Protein and Genetic VariantsThiopurine S-methyltransferase (TPMT: EC 2.1.1.67) is an enzyme that metabolizes immunosuppressive thiopurine medications, used in the treatment of autoimmune diseases, cancer and in transplantation medicine. In some individuals, TPMT enzyme activity is significantly increased or decreased compared to the normal TPMT activity level. Structural and biochemical analyses of the TPMT protein revealed the existence of certain protein variants with altered activity. It has been shown that certain TPMT gene polymorphisms exist, that define different TPMT allozymes. Decreased TPMT enzyme activity can also be a consequence of lower protein synthesis, which depends on the promoter transcription activity. Promoter polymorphisms, such as variable number of tandem repeats (VNTR), can modulate the transcription. Administering thiopurine drugs in patients with certain genetic TPMT variants leads to severe hematologic toxicity. To avoid toxicity, therapy is being modified according to the TPMT genotype (pharmacogenetics). We investigated the polymorphisms in exons and regulatory elements (promoter) of the TPMT gene which affect TPMT enzyme activity in the Serbian population. We used PCR-based methodology and sequencing in the detection of genetic variants on TPMT gene. We showed that genetic variants in exons account for 7.5% of all TPMT variants with decreased enzyme activity. The therapy for patients with these pharmacogenetic markers was modified, which contributed to the efficiency of treatment. Functional assaysin vitroshowed that the TPMT promoter activity and, therefore, the quantity of TPMT protein synthesized, depended on the architecture of VNTRs (i.e. number and type) in the promoter. Promoter of the TPMT gene specifically responds to mercaptopurine treatment of K562 cells in a VNTR-dependent manner. Study of DNA-protein interactions revealed that Sp1 and Sp3 transcription factors interact with VNTRs. Our research pointed out that the VNTR promoter region of the TPMT gene could become a new pharmacogenetic marker, clinically significant for the individualization of thiopurine therapy.
MARP Protein Family: A Possible Role in Molecular Mechanisms of Tumorigenesis
Authors: Kojić Snežana 
Published: August 2010

MARP Protein Family: A Possible Role in Molecular Mechanisms of TumorigenesisThe MARP (muscle ankyrin repeat protein) family comprises three structurally similar proteins: CARP/Ankrd1, Ankrd2/Arpp and DARP/Ankrd23. They share four conserved copies of 33-residue ankyrin repeats and contain a nuclear localization signal, allowing the sorting of MARPs to the nucleus. They are found both in the nucleus and in the cytoplasm of skeletal and cardiac muscle cells, suggesting that MARPs shuttle within the cell enabling them to play a role in signal transduction in striated muscle. Expression of MARPs is altered under different pathological conditions. In skeletal muscle, CARP/Ankrd1 and Ankrd2/Arpp are up-regulated in muscle in patients suffering from Duchene muscular dystrophy, congenital myopathy and spinal muscular atrophy. Mutations inAnkrd1gene (coding CARP/Ankrd1) were identified in dilated and hypertrophic cardiomyopathies. Altered expression of MARPs is also observed in rhabdomyosarcoma, renal oncocytoma and ovarian cancer. In order to functionally characterize MARP family members CARP/Ankrd1 and Ankrd2/Arpp, we have found that both proteins interact with the tumor suppressor p53 bothin vivoandin vitroand that p53 up-regulates their expression. Our results implicate the potential role of MARPs in molecular mechanisms relevant to tumor response and progression.
The Role of GSTM1 and GSTT1 Polymorphism in Patients with Renal Cell Carcinoma
Authors: Ćorić Vesna , Plješa-Ercegovac Marija , Matić Marija , Krivić Biljana , Šuvakov Sonja , Tulić Cane , Mimić-Oka Jasmina , Simić Tatjana 
Published: August 2010

The Role of GSTM1 and GSTT1 Polymorphism in Patients with Renal Cell CarcinomaMembers of the glutathione S-transferase (GST) superfamily exhibit polymorphic expression. GSTs are investigated as biomarkers of risk for various cancers, including renal cell carcinoma (RCC). The aim of this study was to test the association betweenGSTM1andGSTT1polymorphism and susceptibility to RCC, independently or in conjunction with known risk factors. Genomic DNA was isolated from 182 controls and 76 patients with RCC.GSTM1andGSTT1genotypes were determined by multiplex PCR. Data obtained were analyzed with respect to RCC risk factors including smoking and occupational exposure. The frequency ofGSTM1-null genotype was higher in patients with RCC (60.5%) compared to controls (47.2%).GSTT1-null genotype was found in 28.6% controls and 27.6% of cases.GSTM1-null individuals exhibit 1.9-fold increased risk of RCC (95% CI: 1.06-3.33). The presence ofGSTT1active genotype was associated with increased risk of RCC in occupationally exposed subjects when unexposedGSTT1-null subjects were used as a comparison group (OR: 2.48; 95% CI: 1.05-5.86). No association was found between the inactive form ofGSTM1andGSTT1and smoking in RCC patients. In a Serbian cohort of patients, the presence of aGSTM1active genotype is protective against RCC, whereas aGSTT1active genotype increases RCC risk in occupationally exposed subjects.
rRNA Methyltransferases and their Role in Resistance to Antibiotics
Authors: Morić Ivana , Savić Miloje , Ilić-Tomić Tatjana , Vojnović Sandra , Bajkić Sanja , Vasiljević Branka 
Published: August 2010

rRNA Methyltransferases and their Role in Resistance to AntibioticsMethyltransferases (MTases), a large protein superfamily, commonly use S-adenosyl-L-methionine (SAM) as the methyl group donor. SAM-dependant MTases methylate both nucleic acids (DNA, RNA) and proteins, and thus modulate their activity, function and folding. Methylation of G1405 or A1408 nucleotides of 16S rRNA in aminoglycoside-producing microorganisms confers the resistance to their own toxic product(s). This mechanism of resistance has been considered as unique to antibiotics producers until recently. Since 2003, methylation of 16S rRNA as a mechanism of resistance is increasingly emerging in pathogenic bacteria. This represents a major threat towards the usefulness of aminoglycosides in the clinical practice. A potential solution to the problem involves the design of novel compounds that would act against new ribosomal targets. The second approach to the issue includes the development of resistance MTases' inhibitors, with the idea to prevent them from modifying the bacterial rRNA, and thus reinstate the therapeutic power of existing aminoglycosides. As the latter approach has considerable potential, it is obvious that fundamental research related to protein expression, in-depth understanding of the mechanism of action and resolving a tertiary structure of 16S rRNAs MTases are prerequisites for application in medicine.
Editorial
Authors: Mach Mojmír 
Published: August 2010

Editorial
The year 2010 is not gone yet but we already know that this year will be associated with one of the biggest losses in pharmacology and toxicology. On Tuesday, April 13, 2010, at the age of 97, one of the most influential pharmacologists in Eastern Europe, Professor Helena Rašková has left us. Her wisdom, generosity, kindness and hard work made it possible to create a positive environment for research and education, although sometimes she had to fight hard. Her incredible story "How I became a Pharmacologist" wroted by Prof. Rašková herself documents how rich and inspirational her life was [Rašková H. (1997). Pharmacology & Toxicology 80: 255-261]. In this issue you will find mostly works of her colleagues and friends who would like to pay homage to the mother and grandmother of Czecho-Slovak pharmacology and toxicology and to the wonderful person who Prof. Helena Rašková was. She touched many people in her lifetime, affected many and will truly be missed.
Obituary for Prof. Helena Rašková, MD., DSc., Dr.h.c. *2 January, 1913 †13 April, 2010
Authors: Navarová Jana , Ujházy Eduard 
Published: August 2010

Obituary for Prof. Helena Rašková, MD., DSc., Dr.h.c. *2 January, 1913 †13 April, 2010
Professor Rašková had been a member, and after her retirement, an honorary member of a number of scientific societies and editorial boards in Slovakia as well as abroad. To provide a list of all decorations and honours given to Professor Rašková would take a lot of space. Of them, let us mention only that she had been decorated with the Finnish Sibelius Medal, the French medal of the of Académie de Lutec, the Gold Medal of European Pharmacological Societies for her contributions to the development of world pharmacology, as well as all the decorations obtained from the Slovak Academy of Sciences: Gold Medal of the Slovak Academy of Sciences, Honourable Gold Medal of the Slovak Academy of Sciences for her contributions to biological sciences, and the Memorable Medal of the Slovak Academy of Sciences. Professor Rašková was an honorary member of the Learned Society of the Slovak Academy of Sciences
Preclinical electrogastrography in experimental pigs
Authors: Květina Jaroslav , Varayil Jithinraj , Ali Shahzad , Kuneš Martin , Bureš Jan , Tachecí Ilja , Rejchrt Stanislav , Kopáčová Marcela 
Published: August 2010

Preclinical electrogastrography in experimental pigsSurface electrogastrography (EGG) is a non-invasive means of recording gastric myoelectric activity or slow waves from cutaneous leads placed over the stomach. This paper provides a comprehensive review of preclinical EGG. Our group recently set up and worked out the methods for EGG in experimental pigs. We gained our initial experience in the use of EGG in assessment of porcine gastric myoelectric activity after volume challenge and after intragastric administration of itopride and erythromycin. The mean dominant frequency in pigs is comparable with that found in humans. EGG in experimental pigs is feasible. Experimental EGG is an important basis for further preclinical projects in pharmacology and toxicology.
Toxicity of perfluorinated carboxylic acids for aquatic organisms
Authors: Tichý Miloň , Valigurová Radka , Čabala Radomír , Uzlová Rut , Rucki Marián 
Published: August 2010

Toxicity of perfluorinated carboxylic acids for aquatic organismsToxicity of perfluorinated carboxylic acids with carbon chain C8to C12were tested with oligochaetaTubifex tubifex.Toxicity was evaluated as the exposure time ET50from onset of damage of the oligochaeta in saturated aqueous solutions. The ET50fluctuated between 25 and 257 minutes. No statistically significant difference was found among the C8, C9and C12acids (ET50between 143 and 257 minutes with large standard deviation). The acids with carbon chain C10and C11induced the effect significantly quicker (25 to 47 minutes). No acute toxicity measured in the three-minute test was observed in any case.
A meta-analysis of medicinal plants to assess the evidence for toxicity
Authors: Chen Sarah , Vieira Amandio 
Published: August 2010

A meta-analysis of medicinal plants to assess the evidence for toxicityToxicity of phytochemicals, plant-based extracts and dietary supplements, and medicinal plants in general, is of medical importance and must be considered in phytotherapy and other plant uses. We show in this report how general database analyses can provide a quantitative assessment of research and evidence related to toxicity of medicinal plants or specific phytochemicals. As examples, several medicinal plants are analyzed for their relation to nephrotoxicity and hepatotoxicity. The results of analyses in different databases are similar, and reveal the two best-established toxic effects among the group of plants that were examined: nephrotoxicity ofAristolochia fangchiand hepatotoxicity ofLarrea tridentata.
Directed Cooperation in Multihop Wireless Sensors Network
Authors: Nikodem Jan , Klempous Ryszard , Nikodem Maciej , Woda Marek , Chaczko Zenon 
Published: August 2010

Directed Cooperation in Multihop Wireless Sensors NetworkThis paper proposes a relational abstraction for Wireless Sensors Network where node can identify its neighbors around it. Because of limited radio link range only some of nodes have a direct contact with the base station and transmission is carried out in a multihop way so information is send from one node to another towards the BS. We propose a relation π that represents cooperation between nodes and takes advantages of topological properties of the network. Based on the hop-distance from the BS any nodekcan determine a setN<(k) that consists of nodes to which/kshould send messages in order to retain a data-flow direction towards the BS.
Statechart-based Controllers Synthesis in FPGA Structures with Embedded Array Blocks
Authors: Łabiak Grzegorz , Borowik Grzegorz 
Published: August 2010

Statechart-based Controllers Synthesis in FPGA Structures with Embedded Array BlocksStatechart diagrams, in general, are visual formalism for description of complex systems behaiour. Digital controllers, which act as reactive systems, can be very conveniently modeled with statecharts and efficiently synthesized in modern programmable devices. The paper presents in details syntax and semantics of statecharts and new implementation scheme. The issue of statecharts synthesis is not still ultimately solved. Main feature of the presented approach is the transformation of statechart diagrams into Finite State Machine, and through KISS format, functional decomposition and mapping into Embedded Memory Blocks. Embedded Memory are part of the modern programmable devices.
Tabu Search Algorithm for Survivable Network Design Problem with Simultaneous Unicast and Anycast Flows
Authors: Gładysz Jakub , Walkowiak Krzysztof 
Published: August 2010

Tabu Search Algorithm for Survivable Network Design Problem with Simultaneous Unicast and Anycast FlowsIn this work we focus on the problem of survivable network design for simultaneous unicast and anycast flows. This problem follows from the growing popularity of network services applying the anycast paradigm. The anycasting is defined as one-to-one-of-many transmission and is applied in Domain Name Service (DNS), peer-to-peer (P2P) systems, Content Delivery Networks (CDN). In this work we formulate two models that enables joint optimization of network capacity, working and backup connections for both unicast and anycast flows. The goal is to minimize the network cost required to protect the network against failures using the single backup path approach. In the first model we consider modular link cost, in the second we are given a set of link proposal and we must select only one of them. Because these problems are NP-hard, therefore optimal solutions of branch-and-bounds or branch-and-cut methods can be generated for relatively small networks. Consequently, we propose a new heuristic algorithm based on Tabu Search method. We present results showing the effectiveness the proposed heuristic compared against optimal results. Moreover, we report results showing that the use of anycast paradigm can reduce the network cost.
Sensor Actor Network Modeling utilizing the Holonic Architectural Framework
Authors: Chiu Christopher , Chaczko Zenon , Moses Perez 
Published: August 2010

Sensor Actor Network Modeling utilizing the Holonic Architectural FrameworkThis paper discusses the results of utilizing advanced EKM modeling techniques to manage Sensor-Actor networks (SANETs) based upon the Holonic Architectural Framework. EKMs allow a quantitative analysis of an algorithmic artificial neural network process by using an indirect-mapping EKM to self-organize from a given input space to administer SANET routing and clustering functions with a control parameter space. Results demonstrate that in comparison to linear approximation techniques, indirect mapping with EKMs provide fluid control and feedback mechanisms by operating in a continuous sensory control space - thus enabling interactive detection and optimization of events in real-time environments.
Pseudo Bayesian and Linear Regression Global Thresholding
Authors: Aboura Khalid 
Published: August 2010

Pseudo Bayesian and Linear Regression Global ThresholdingClassification is an important task in image analysis. Simply recognizing an object in an image can be a daunting step for a computer algorithm. The methodologies are often simple but rely heavily on the thresholding of the image. The operation of turning a color or gray-scale image into a black and white image is a determining step in the effectiveness of a solution. Thresholding methods perform differently in various problems where they are often used locally. Global thresholding is a difficult task in most problems. We highlight a pseudo Bayesian and a linear regression global thresholding methods that performed well in an engineering problem. The same approaches can be used in biomedical applications where the environment is better controlled.
Algorithms for reliable networks deployment in mesh topology with flow control
Authors: Debita Grzegorz , Staniec Kamil 
Published: August 2010

Algorithms for reliable networks deployment in mesh topology with flow controlThe article presents innovative methods for designing and controlling topology in mesh networks. The authors of the paper simulate the procedure of deploying a mesh structure and prove that the shortest path for such a network is also one with the least number of hops in the sense of intermediate nodes.
Operational characteristic of wireless WiMax and IEEE 802.11x systems in underground mine environments
Authors: Kowal Michał , Kubal Sławomir , Piotrowski Piotr , Zieliński Ryszard 
Published: August 2010

Operational characteristic of wireless WiMax and IEEE 802.11x systems in underground mine environments
The paper presents research results pertaining to transmission parameters of wireless communication systems, based on WiMax and IEEE 802.11x radio interfaces. Research was performed in severe operating conditions of an underground mine - testing various parameters, such as: throughput, delays and maximum range.
ChemInform Abstract: Efficient Conversion of (S)‐Methionine into (R)‐Garner Aldehyde.
Authors: KUMAR J. S. R. , DATTA A. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of a cis‐Conformationally Restricted Peptide Bond Isostere and Its Application to the Inhibition of the HIV‐1 Protease.
Authors: ABELL A. D. , FOULDS G. J. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of 12 Stereochemically and Structurally Diverse C‐ Trisaccharides.
Authors: SUTHERLIN D. P. , ARMSTRONG R. W. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Ring Opening Rearrangement Reaction of 6β‐Hydroxytropinone.
Authors: CHEN Z. , GONZALEZ M. D. , BLUNDELL P. , MELTZER P. C. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Efficient Synthesis of (.+‐.)‐N‐Boc‐exo‐2‐(methoxycarbonyl)‐7‐ azabicyclo(2.2.1)heptane, a Versatile Intermediate for the Synthesis of Epibatidine and Epiboxidine.
Authors: SINGH S. , BASMADJIAN G. P. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A New Method for the Formation of the Imidazo(4′,5′;3,4)pyrido(2,3‐b) indole Ring: Formal Synthesis of the Alkaloids from Marine Origin Grossularines‐1 and ‐2.
Authors: MOLINA P. , FRESNEDA P. M. , SANZ M. A. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: High‐Yield Protection of Purine Ribonucleosides for H‐Phosphonate RNA Synthesis
Authors: ZHANG X. , ABAD J.‐L. , HUANG Q. , ZENG F. , GAFFNEY B. L. , JONES R. A. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Cycloadditions of Cephalosporins. A Comprehensive Study of the Reaction of Cephalosporin Triflates with Olefins, Acetylenes, and Dienes to Form (2 + 2) and (4 + 2) Adducts.
Authors: ELLIOTT R. L. , NICHOLSON N. H. , PEAKER F. E. , TAKLE A. K. , RICHARDSON C. M. , TYLER J. W. , WHITE J. , PEARSON M. J. , EGGLESTON D. S. , HALTIWANGER R. C. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Preparation of 4,5‐Cyclopropylsugar Derivatives: Application to the Stereocontrolled Synthesis of Bottom Half (C7‐C16) Segment of Lasonolide A.
Authors: GURJAR M. K. , CHAKRABARTI A. , RAO B. V. , KUMAR P. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.