bluuebunny/crossref_metadata_2025_split huggingface dataset:
Risk factors of survival after liver transplantation for HCC: a multivariate single‐center analysis
Authors: Hoffmann Katrin , Hinz Ulf , Hillebrand Norbert , Radeleff Boris A. , Ganten Tom M. , Schirmacher Peter , Schmidt Jan , Büchler Markus W. , Schemmer Peter 
Published: April 2011

Hoffmann K, Hinz U, Hillebrand N, Radeleff BA, Ganten TM, Schirmacher P, Schmidt J, Büchler MW, Schemmer P. Risk factors of survival after liver transplantation for HCC: a multivariate single‐center analysis. Clin Transplant 2011: 25: E541–E551. © 2011 John Wiley & Sons A/S.Abstract:  Background:  The selection criteria for liver transplantation (LT) in patients with hepatocellular cancer (HCC) are well defined. Increasing evidence suggests that the effectiveness of pre‐transplant bridging influences the individual course after LT significantly. Thus, the aim of this study was to determine its impact on tumor progression during waiting time and identify patient subgroups with favorable oncological long‐term outcome.Methods:  Prospectively collected data of 78 consecutive patients undergoing LT for HCC between 2001 and 2007 were analyzed retrospectively. Survival rates were assessed using the Kaplan–Meier estimate. Clinicopathologic prognostic factors were identified by Cox regression analysis.Results:  After 48.9 months of median follow‐up, the five‐yr overall survival rate is 57% with a five‐yr recurrence‐free survival rate of 74%. Progressive disease (PD) during bridging was developed in 32% of patients, and a trend toward impaired overall survival in patients with PD before LT was detected in multivariate analysis (p = 0.073). HCC ≥3 cm was associated with a three times increased risk of recurrent disease. Neither fulfillment of MILAN criteria nor bridging with transarterial chemoembolization had an impact on the outcome.Conclusion:  PD during waiting time influences the oncological course after LT. However, even with an increasing organ shortage, further studies are warranted to define clear selection criteria based on the biological tumor behavior and allow a more personalized treatment.
Anatomy of the right liver lobe: a surgical analysis in 124 consecutive living donors
Authors: Bageacu S. , Abdelaal A. , Ficarelli S. , ElMeteini M. , Boillot O. 
Published: April 2011

Bageacu S, Abdelaal A, Ficarelli S, ElMeteini M, Boillot O. Anatomy of the right liver lobe: a surgical analysis in 124 consecutive living donors. Clin Transplant 2011: 25: E447–E454. © 2011 John Wiley & Sons A/S.Abstract:  Background:  Understanding anatomic variations of the right lobe is fundamental in adult to adult living donor liver transplantation.Methods:  We analysed anatomy in 124 right liver (RL) donors.Results:  Portal vein: normal anatomy was found in 85.5% donors. In 14.5% the main right portal vein (PV) was absent. Hepatic artery: single arterial inflow of the RL was identified in 96% of donors. In 4% two arterial stumps were found. Bile duct: classic anatomy was identified in 50.8% of donors; 9.7% had a trifurcation of the common bile duct; in 7.2% the right anterior and in 15.3% the right posterior bile duct opened into the left bile duct; one segmental bile duct opened directly into the common bile duct in 12.1% and two segmental bile ducts in 4.8%. Hepatic veins (HV): in 74.3% the right HV was the single outflow; in 24.2% significant accessory HV (>5 mm) were preserved, in 2.4% the middle HV was harvested. We found that patients with PV variations had high incidence of multiple bile ducts (88.9%) while patients with single right PV had lower incidence (42.4%) (p = 0.00026).Conclusion:  While anatomic variations in the RL donor were common, no contraindication to RL harvesting was noted in this study.
Doppler tissue imaging in children following cardiac transplantation: A comparison to catheter derived hemodynamics
Authors: Goldberg David J. , Quartermain Michael D. , Glatz Andrew C. , Hall E. Kevin , Davis Erin , Kren Stephanie A. , Hanna Brian D. , Cohen Meryl S. 
Published: April 2011

Goldberg DJ, Quartermain MD, Glatz AC, Hall EK, Davis E, Kren SA, Hanna BD, Cohen MS. DTI in children following cardiac transplantation: A comparison to catheter derived hemodynamics.  Pediatr Transplantation 2011: 15: 488–494. © 2011 John Wiley & Sons A/S.Abstract:  Previous studies have demonstrated a correlation between E:Ea and ventricular filling pressure in adults after heart transplantation. We sought to determine if E:Ea correlates with filling pressure after heart transplantation in children. A prospective analysis of children who have undergone heart transplantation was performed. Inflow and myocardial velocities were recorded and compared to catheter‐derived filling pressures and rejection status. We performed 61 studies in 49 subjects. No correlation was found between septal E:Ea and PCWP (r = 0.14, p = 0.28); or between lateral tricuspid E:Ea and mean RAp (r = 0.04, p = 0.79). However, the mean PCWP was higher among subjects with elevated septal E:Ea (>12) compared to normal E:Ea (12.3 ± 2.8 mmHg vs. 10.1 ± 2.9 mmHg, p = 0.02). Similarly, mean RAp was higher among subjects with an elevated lateral tricuspid E:Ea (>10) compared to normal lateral tricuspid E:Ea (7.7 ± 2.1 mmHg vs. 6.0 ± 2.4 mmHg, p = 0.04). Elevated septal E:Ea was also associated with high‐grade cellular rejection (OR = 17.3 [95% CI 1.4–221], p = 0.028). In children following heart transplant, E:Ea does not correlate well with the range of filling pressures seen after pediatric heart transplantation. However, a septal E:Ea > 12 is associated with elevated PCWP and high grade cellular rejection and a lateral tricuspid E:Ea > 10 is associated with elevated mean RAp.
Mobilized allogeneic peripheral stem/progenitor cell apheresis with Spectra Optia v.5·0, a novel, automatic interface‐controlled apheresis system: results from the first feasibility trial
Authors: Brauninger S. , Bialleck H. , Thorausch K. , Seifried E. , Bonig H. 
Published: April 2011

Background and Objectives  G‐CSF mobilized peripheral blood stem/progenitor cells are frequently used for allogeneic transplantation. Available manual apheresis systems generate stem cell products of consistently high quality. Short‐comings include need for continuous interface monitoring/adjustment, interface instability in donors with inconsistent blood flow, high collection variability, high platelet loss, and failure to electronically document apheresis parameters.Material and Methods  A fundamentally different, novel apheresis system, Spectra Optia v.5·0, featuring optical sensors, which provide real‐time automatic interface and product collect line control, and newly developed tubing sets, was designed to address these short‐comings. In a prospective validation study, 30 healthy volunteer stem cell donors were subjected to apheresis with Spectra Optia to test feasibility and effectiveness. Results were compared to 608 historic first‐day allogeneic aphereses with COBE Spectra MNC.Results  Usability and function of automatic interface control of Spectra Optia were good. Most collection parameters, including collection efficiency and product size, were similar. Cells were viable and provided timely engraftment. Platelet loss with Spectra Optia was 25% less than with COBE Spectra MNC. Products contained fewer erythrocytes, but more granulocytes.Conclusion  The automatic apheresis system Spectra Optia is functional and user‐friendly. Thus Spectra Optia aphereses are associated with similar, and equally variable, collection efficiencies as COBE Spectra MNC.
Detection of mRNA by reverse‐transcription PCR as an indicator of viability in Phytophthora ramorum
Authors: Chimento A. , Cacciola S. O. , Garbelotto M. 
Published: April 2011

SummaryIn the last few decades, the use of molecular tools has greatly improved the efficiency of plant disease diagnosis. However, one of the major setbacks of most molecular diagnostic approaches is their inability to differentiate between dead and viable pathogens. We propose a new strategy for the detection of plant pathogens, based on the use of mRNA as a viability marker, on the basis that mRNA degradation in dead cells is significantly more rapid than that of DNA. A real‐time reverse‐transcription PCR (RT‐PCR) assay targeting the mRNA of the subunit I of the cytochrome oxidase gene was designed for Phytophthora ramorum, the causal agent of sudden oak death and ramorum blight. In controlled laboratory tests, the developed RT‐PCR assay did not detect the target mRNA a week after the pathogen had been killed by rapid lyophilization, while DNA of the pathogen could still be detected 3 months after the pathogen had died. The RT‐PCR assay was then compared with a traditional culturing approach using PARP selective medium and two nested real‐time PCR techniques on symptomatic California bay laurel leaves. Samples were either collected in three different sites in July, or in the same site but in three different seasons. Overall, RT‐PCR results showed less positive samples than DNA‐based nested PCR techniques (p < 0.0001), but more than culturing (p = 0.017). Nested PCR‐positive but RT‐PCR‐negative samples may not be viable. On the other hand, RT‐PCR‐positive but culture‐negative samples may be viable but dormant. A comparative analysis of the results indicated that RT‐PCR and culturing provide comparable results when climatic conditions are optimal for the pathogen, but RT‐PCR may be the most accurate approach to determine pathogen viability when climatic conditions are less than optimal for the pathogen.
Root lesions in large loblolly pine (Pinus taeda L.) following inoculation with four root‐inhabiting ophiostomatoid fungi
Authors: Matusick G. , Somers G. L. , Eckhardt L. G. 
Published: April 2011

SummaryLoblolly pine decline, characterized by deteriorating root systems leading to shortening and thinning of foliage, has been observed throughout portions of the south‐eastern United States. Several root‐inhabiting ophiostomatoid fungi, including Leptographium procerum, Leptographium terebrantis, Leptographium serpens, and Grosmannia huntii are associated with lateral root damage on declining loblolly pine. Trees of various ages were inoculated in primary lateral roots during fall (2006 and 2007) and spring (2007 and 2008). All fungi caused a darkened, resin‐filled lesion on the surface of the phloem, extending into the xylem that was larger than that of controls. Only lesions associated with G. huntii infection were significantly larger in the spring season, compared with the fall. Grosmannia huntii was found to be the most virulent fungus, causing lesions that were longer, deeper and larger than all other fungal species during the spring and larger than L. terebrantis and L. procerum in the fall. Leptographium serpens was the second most virulent fungal species, causing lesions larger than L. procerum and L. terebrantis (with the exception of lesion depth) during both seasons. These tests indicate that G. huntii and L. serpens are significant root pathogens, capable of causing considerable damage, while L. terebrantis and L. procerum may be less virulent. Depending on the actions of their vectors, G. huntii and L. serpens may be responsible for significant root deterioration and tree disease.
Controlling ‘bois noir’ disease on grapevine: does the timing of herbicide application affect vector emergence?
Authors: Kehrli P. , Delabays N. 
Published: April 2011

AbstractBois noir is an important grapevine yellows disease that can cause serious economical losses in European grapevine production. Hyalesthes obsoletus Signoret (Hemiptera, Cixiidae) is the principal vector of bois noir in Switzerland and stinging nettle (Urtica dioica) is its favourite host plant species in vineyards. As bois noir disease can hardly be cured and direct control measures against H. obsoletus are ineffective, viticultural control practices target stinging nettle, the actual reservoir and source of both the pathogen and its vector. Currently, it is recommended to apply herbicides against stinging nettle at the end of the season to kill developing H. obsoletus nymphs. To verify if this late period of herbicide application is justified, stinging nettle patches were treated with glyphosate in the autumn, in the spring or were left untreated as a control. Herbicide applications at both dates controlled the growth of stinging nettle very well in the subsequent summer, although the autumnal treatment was slightly more efficient. To study glyphosate’s direct impact on the development of H. obsoletus nymphs, emergence traps were placed directly in the centre of treated and untreated stinging nettle patches. There was no significant difference among the three treatments in the total number of adults emerging. Thus, an aerial application of glyphosate in either spring or autumn did not inhibit the nymphs’ development on the roots of stinging nettle in the soil. Our results challenge current recommendations of applying herbicides against stinging nettle at the end of the season and suggest that stinging nettle could also be controlled in spring, alike other viticultural weeds.
Klotho protein diminishes endothelial apoptosis and senescence via a mitogen‐activated kinase pathway
Authors: Maekawa Yoshihiro , Ohishi Mitsuru , Ikushima Masashi , Yamamoto Koichi , Yasuda Osamu , Oguro Ryosuke , Yamamoto‐Hanasaki Hiroko , Tatara Yuji , Takeya Yasushi , Rakugi Hiromi 
Published: April 2011

Aim:  Mice that carry the Klotho mutation (KL‐/‐) manifest diverse age‐related disorders similar to those observed in humans. Thus, the Klotho protein might function as an anti‐aging hormone in mammals. Recently, we reported that Klotho recombinant protein attenuated apoptosis and cellular senescence in endothelial cells, but the mechanism remained unclear. Here, we designed an in vitro study to test whether inhibitors of extracellular signal‐regulated kinase and mitogen‐activated kinase kinase could affect Klotho regulation of apoptosis and cellular senescence.Methods:  Cellular senescence was investigated in human umbilical vein endothelial cells treated with or without Klotho recombinant protein, and with or without inhibitors of mitogen‐activated kinases. Senescence was quantified by staining with senescence‐associated β‐galactosidase and by evaluating western blots probed for phosphorylation of mitogen‐activated kinases. Apoptosis was assayed on western probed for p53, p21, and caspase‐3 and ‐9.Results:  The Klotho recombinant protein induced transient phosphorylation of mitogen‐activated kinases within a few minutes. Application of inhibitors of mitogen‐activated kinases attenuated the ability of Klotho to interfere with apoptosis and senescence in endothelial cells.Conclusion:  This study demonstrated that Klotho attenuated cellular apoptosis and senescence in vascular cells via mitogen‐activated kinase kinase and extracellular signal‐regulated kinase pathways. Geriatr Gerontol Int 2011; 11: 510–516.
Synchronous breast invasive ductal carcinoma and endometrial endometrioid adenocarcinoma: Case report
Authors: Yeh Chang‐Ching , Wang Peng‐Hui , Lai Chiung‐Ru , Yen Min‐Shyen , Chao Kuan‐Chong 
Published: April 2011

AbstractDiagnosis of synchronous primary cancers in one patient is a relatively rare event. The well‐known synchronous primary cancers of women are combined ovarian and endometrial cancers, especially when both are the endometrioid cell type. Although breast cancer and endometrial cancer are two common female malignancies, they often occur during the postmenopausal period. Therefore, the possibility of concomitant breast cancer and endometrial cancer in a younger woman (premenopausal) is often neglected. The reported case is an example of this situation. A 37‐year‐old woman was diagnosed with synchronous breast invasive ductal carcinoma and endometrial endometrioid adenocarcinoma. Since this condition is rarely reported, the correlated risk factors are worthy of our attention and the strategy for prevention is highlighted.
No increase in hemorrhagic complications with thromboprophylaxis using low‐molecular‐weight heparin soon after cesarean section
Authors: Watanabe Takashi , Matsubara Shigeki , Usui Rie , Izumi Akio , Kuwata Tomoyuki , Suzuki Mitsuaki 
Published: April 2011

AbstractAim:  Data from Japanese women have been lacking regarding hemorrhagic complications due to low‐molecular‐weight heparin (LMWH) as thromboprophylaxis. Thus, targeting Japanese women, we made an effort to determine: (i) whether the administration of LMWH soon after cesarean section increases the risk of hemorrhagic complications compared to that of unfractionated heparin; and (ii) how it elongates the activated partial thromboplastin time.Materials and Methods:  We administered unfractionated heparin in the first half of the study period, and LMWH in the latter half. We examined: (i) the incidence rate of hemorrhagic complications; and (ii) preoperative and postoperative activated partial thromboplastin time, and we compared these in cases using unfractionated heparin and LMWH.Results:  No clinically discernable thromboembolism occurred in either group. Hemorrhagic complications occurred in two of 140 women in the unfractionated heparin group and one of 131 women in the LMWH group. LMWH prolonged the activated partial thromboplastin time from 29.8 ± 2.6 to 34.8 ± 4.0 s. This prolongation was significantly shorter than that with unfractionated heparin (from 30.2 ± 2.6 to 36.5 ± 6.2 s).Conclusions:  Compared with thromboprophylaxis with unfractionated heparin, thromboprophylaxis with early administration of LMWH after cesarean section did not increase the incidence of hemorrhagic complications and caused less prolongation of the activated partial thromboplastin time in Japanese women.
Interstitial pregnancy treated by transcervical aspiration of the gestational sac combined with systemic and local administration of methotrexate
Authors: Kato Sousuke , Tanaka Tomohito , Terai Yoshito , Yamashita Yoshiki , Ohmichi Masahide 
Published: April 2011

AbstractInterstitial pregnancy, which is a rare form of tubal ectopic pregnancy, can grow larger than those within the fallopian tube because the surrounding myometrium is more expandable than the tube; many cases are advanced and treated with surgical resection or a large amount of methotrexate (MTX). This report presents a case of an advanced interstitial pregnancy treated with systemic MTX and laparoscopic local MTX injection combined with transcervical aspiration of the gestational sac. A 27‐year‐old nulliparous female presented with an interstitial pregnancy. Serum human chorionic gonadotropin (hCG) level was 90 000 IU/L. MTX was given systemically (50 mg/m2 i.m.) and the gestational sac was aspirated transcervically under laparoscopic guidance followed by local injection of 25 mg MTX. The patient received a total of only 95 mg MTX. The gestational sac disappeared and serum hCG became undetectable. The patient became pregnant spontaneously six months later, and delivered a live 2482‐g infant in good condition by planned cesarean section at 36 weeks and 3 days of gestation. No defect of the myometrium was seen during the surgery. This therapy may be effective for interstitial pregnancy and can be performed safely with laparoscopy.
Bacterial monocultures, propionate, butyrate and H2O2 modulate the expression, secretion and structure of the fasting‐induced adipose factor in gut epithelial cell lines
Authors: Grootaert Charlotte , Van de Wiele Tom , Van Roosbroeck Ilse , Possemiers Sam , Vercoutter‐Edouart Anne‐Sophie , Verstraete Willy , Bracke Marc , Vanhoecke Barbara 
Published: April 2011

SummaryPrevious research showed that an intestinal microbial community represses the fasting‐induced adipose factor (FIAF) in the gut epithelium, thereby increasing fat storage in the host. This study was designed to investigate the overall effect of different bacterial species and metabolites on FIAF in intestinal (Caco‐2, HT‐29 and HCT‐116) and hepatic (HepG2) cancer cell lines. First, we showed that FIAF was present in different isoforms, and secreted as N‐glycosylated proteins, exclusively at the basal side of the cell monolayer. Second, co‐incubation of cell lines with bacterial monocultures and metabolites altered both FIAF production and isoform appearance. Propionate and/or butyrate treatment increased FIAF expression and cleavage in all tested cell lines. In contrast, different bacteria induced cell line‐specific FIAF modulation. Clostridium perfringens induced FIAF isoform changes in Caco‐2 cells. Enterococcus faecalis and Bacteroides thetaiotaomicron treatment resulted in cell line‐specific FIAF increases, whereas Escherichia coli significantly decreased FIAF expression in HCT‐116 cells. Treatment with H2O2 and peroxide‐producing E. faecalis strains induced FIAF isoform changes in Caco‐2 cells. Since bacteria and bacterial metabolites alter both FIAF production and isoform appearance, further investigation may reveal an important role for bacteria in FIAF‐regulated physiological processes, such as cell differentiation and fat metabolism.
Proteome changes in the initial bacterial colonist during ecological succession in an acid mine drainage biofilm community
Authors: Mueller Ryan S. , Dill Brian D. , Pan Chongle , Belnap Christopher P. , Thomas Brian C. , VerBerkmoes Nathan C. , Hettich Robert L. , Banfield Jillian F. 
Published: April 2011

SummaryProteomes of acid mine drainage biofilms at different stages of ecological succession were examined to understand microbial responses to changing community membership. We evaluated the degree of reproducibility of the community proteomes between samples of the same growth stage and found stable and predictable protein abundance patterns across time and sampling space, allowing for a set of 50 classifier proteins to be identified for use in predicting growth stages of undefined communities. Additionally, physiological changes in the dominant species, Leptospirillum Group II, were analysed as biofilms mature. During early growth stages, this population responds to abiotic stresses related to growth on the acid mine drainage solution. Enzymes involved in protein synthesis, cell division and utilization of 1‐ and 2‐carbon compounds were more abundant in early growth stages, suggesting rapid growth and a reorganization of metabolism during biofilm initiation. As biofilms thicken and diversify, external stresses arise from competition for dwindling resources, which may inhibit cell division of Leptospirillum Group II through the SOS response. This population also represses translation and synthesizes more complex carbohydrates and amino acids in mature biofilms. These findings provide unprecedented insight into the physiological changes that may result from competitive interactions within communities in natural environments.