bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of sewage sludge composts with straw or ash on oribatid mites (Acari, Oribatida) from pine forest litter in laboratory conditions
Authors: Klimek Andrzej , Chachaj Bogusław , Kosakowski Leszek 
Published: December 2011

Influence of sewage sludge composts with straw or ash on oribatid mites (Acari, Oribatida) from pine forest litter in laboratory conditions
In a substrate obtained from municipal sewage sludge composts, the C:N ratio was decreased during the 12-month experiment. The amount of forest litter (layer 1 or 2 cm thick), used for fauna introduction, did not influence significantly the mean abundance of oribatid mites in sewage sludge compost. Oribatid mites tolerated compost alone or with straw, but were negatively influenced by addition of wood-ash. Abundance of oribatid mites in compost alone was increased from the 3rd month, and its maximum occurred in the 10th month. Abundance of oribatid mites in compost with straw was clearly increased since the 10th-12th month of the experiment. Generally the abundance of oribatid mites was mainly affected by Ramusella mihelcici, which was most abundant in compost alone and with straw (maximum abundance was above 100 000 individuals · m-2). However, species diversity of the mites was very low at the end of the experiment.
Lifestyle and Living Physical Activity as a Part of an Active Way of Life in the Czech Republic
Authors: Sekot Aleš 
Published: December 2011

Lifestyle and Living Physical Activity as a Part of an Active Way of Life in the Czech RepublicAn integral part of a reasonable, creative, valuable, and positive way of life is also personal responsibility for individual physical and psychical shape and good health: a sound state of body and soul, freedom from disease, bodily conditions, and a wish for health and happiness. In this context we are increasingly aware of the great importance of sport not only as a part of mass culture, but broadly understood, for individual and social health and well-being. Physical activity of people plays an increasingly more important role in scientific interest regarding the way of life of contemporary society, and it is a very important factor in the process of officiating the level of a healthy and active lifestyle, quality of life, and health in general. The indispensable role of physical activity in the course of human life is permanently and scientifically confirmed in the context of the prevention of obesity.The development of a sedentary life style is the result of a socialization process towards physical inactivity developed in youth and continued into adulthood. At the present we face in our cultural settings an apparent tendency: people are more and more individualized, losing the beneficial impacts of community activities, involved in passive way of life, and lacking proper level of physical activities and active sport. The phenomenon of physical activity has also been considered from the perspective of the EURO-PRE-VOB project: accenting built environmental as an aspect of way of life. The Czech Republic is, on the level of mass sport, strongly influenced by the existence of new development of the city structure, including fitness centres, bicycling paths, roller-skating stadiums, beach volleyball playing fields, and golf courses. The national support of sport is, nowadays, divided into the support of top sports, performance sports, and new waves in sports (e.g. sports for everyone - for all), sports for school, which are then on the regional level (and by the various sport organizations) subsidized from other sources and, moreover, not coordinately.
The Recreational Function of the Dunajec River and the Pieniny Mountains in Transformation Dynamics of Natural and Social Environment
Authors: Eliašová Darina , Stankiewicz Bogusław 
Published: December 2011

The Recreational Function of the Dunajec River and the Pieniny Mountains in Transformation Dynamics of Natural and Social Environment
The article focuses on the recreational function of the Dunajec River and the Pieniny Mountains in regard to their use for sustainable tourism under the Slovak-Polish cross-border cooperation. Based on investigation and implemented research, it reflects on the debated topic in the dimensions of Slovak-Polish relations with the focus on tourism, pursued by the authors in their educational activities, research and publications. Their joint presentational output is a comparison of past and present recreational and related activities, proposing possible methods in tourism development in this tourist destination.
Sustainability as a Tool for Increasing Competitiveness
Authors: Štefaňák Peter 
Published: December 2011

Sustainability as a Tool for Increasing Competitiveness
One of the main aims of companies is to reach quality of products and services. In particular, good quality of services is a highly rated factor so the price often stands behind. Nevertheless, many companies focus on the quality as the customer is willing to pay for it. The company is more competitive; however, they must strive to achieve continual improvements. Accepting the principles of sustainable development is one of the instruments or strategies to reach competitive growth. Currently this notion is being profaned and often considered only as a simple phrase, but many companies from different spheres, such as tourism, electrical engineering, retail, civil engineering, etc., show its real benefits. Companies create strategies of sustainable development following company competitiveness.
Damage domains of chemically reacting industrial facilities. An adequate identification model for Bhopal‐like scenarios
Authors: Benikhlef Taha , Benazzouz Djamel , Izquierdo José M. , Sanchez Miguel 
Published: December 2011

ABSTRACTThis paper focuses on the improvement of solutions to some of the problems that arise in chemical and petrochemical risk assessment studies, namely those associated with potentially undue grouping in sequences of events, caused by different time evolutions. It provides an adequate work‐horse simulation model of risky scenarios able to explore a large amount of transients at a reasonable and viable cost. It is specifically suitable for the transfer of modern dynamic reliability techniques, originated in the nuclear domain, to the chemical engineering environment. As an application, it presents results of a case study to analyse Bhopal‐like scenarios. We found the model adequate to discriminate and filter out the myriad of success scenarios expected in well‐protected installations, screening necessary to focus the risk studies on damage situations. © 2011 Curtin University of Technology and John Wiley & Sons, Ltd.
Surface modification of polypropylene membrane by acrylate epoxidized soybean oil to be used in water treatment
Authors: Palacios‐Jaimes Martha Liliana , Cortes‐Guzman Fernando , González‐Martínez David Alejandro , Gómez‐Espinosa Rosa María 
Published: December 2011

AbstractSurface modification of a microporous polypropylene (PP) membrane was performed by graft polymerization of acrylated epoxidized soybean oil (AESO) using UV radiation. This is a simple, environmentally friendly, and low cost method. The factors affecting the grafting degree such as monomer concentration and reaction time were studied. The morphological and microstructure changes of the membrane were characterized by scanning electron microscopy (SEM) and information regarding elemental composition was obtained by energy dispersive X‐ray spectroscopy (EDS). Surface functionalization was studied by X‐ray photoelectron spectroscopy (XPS). Thermal properties were analyzed by differential scanning calorimetry (DSC). The hydrophilicity increment was confirmed by pure water contact angle and swelling measurements. The filtration capability of the modified membrane was analyzed by determining color, turbidity, and chemical oxygen demand (COD) removal from a residual raw water. The results indicated that the contact angle of pure water on the grafted membrane decreased from 90° to 57°. The modified membrane shows filtration capability by removing color in 52% and reducing 95% of turbidity. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Novel poly(azomethine‐urethane)s and their polyphenol derivatives derived from aliphatic diisocyanate compound: Synthesis and thermal characterization
Authors: Kaya İsmet , Kamacı Musa 
Published: December 2011

AbstractUntil now, only a few kinds of poly(azomethine‐urethane)s (PAMUs) including aromatic hydroxy benzaldehyde and aminophenol compounds were obtained and studied with thermal degradation steps. However, oligo/polyphenol‐based PAMUs have not been synthesized yet. In this study, some kinds of this class of PAMUs were synthesized in three steps. At the first step polyurethane (PU) was synthesized by the copolymerization reaction of 2,4‐dihydroxybenzaldehyde with hexamethylene diisocyanate (HDI) under argon atmosphere. At the second step, the poly(azomethine‐urethane)s (PAMUs) were obtained by graft copolymerization of the preformed PU with aminophenols (2‐aminophenol, 3‐aminophenol, and 4‐aminophenol). At the last step the obtained PAMUs were converted to the polyphenol derivatives via oxidative polycondensation reaction (OP). The structures of the obtained compounds were confirmed by FTIR, UV‐vis, 1H‐NMR, and 13C‐NMR techniques. The number‐average molecular weight (Mn), weight average molecular weight (Mw) and polydispersity index (PDI) values of the synthesized compounds were determined by the size exclusion chromatography (SEC). The synthesized compounds were also characterized by solubility tests, TG‐DTA, and DSC. Fluorescence measurements were carried out in various concentrated DMF solutions to determine the optimum concentrations to obtain the maximal PL intensities. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Development of a compact capillary electrophoresis–chemiluminescence system with ultra‐fast peroxyoxalate reaction to monitor the hydrolysis of rhodamine 6G
Authors: Zhu Jinkun , Shu Lu , Zhang Fan , Li Zicheng , Wang Qingjiang , He Pingang , Fang Yuzhi 
Published: December 2011

ABSTRACTA simple and effective capillary electrophoresis–chemiluminescence (CE–CL) detection system was developed based on an ultra‐fast bis(2,4,6‐trichlorophenyl)oxalate (TCPO) chemiluminescence (CL) reaction (0.6 s duration) that avoided overlapping peaks and peak tailing. Through a series of static injection experiments, this unusually rapid CL reaction was ascribed to the catalytic effect of imidazole in the tetrahydrofuran solvent, which has been rarely utilized in such investigations. A possible mechanism is given to explain the results. Under the optimized conditions, rhodamine 6 G (R6G) and its hydrolysis product (R6G‐COOH) could be efficiently separated through electrophoresis in 7 min, with sensitive CL detection in the proposed CE–CL system. In this way, the alkaline hydrolysis of R6G was monitored, followed by estimation of relative rate constants and activation energy. This finding and application should be helpful in further study for the TCPO CL reaction, and revealed an attractive opportunity for simplifying the CE–CL system, such as in a microchip device. Copyright © 2011 John Wiley & Sons, Ltd.
Synthesis and Characterization of Photoswitchable Fluorescent SiO2 Nanoparticles
Authors: May Florian , Peter Michael , Hütten Andreas , Prodi Luca , Mattay Jochen 
Published: December 2011

AbstractSwitchable fluorescent silica nanoparticles have been prepared by covalently incorporating a fluorophore and a photochromic compound inside the particle core. The fluorescence can be switched reversibly between an on‐ and off‐state via energy transfer. The particles were synthesized using different amounts of the photoswitchable compound (spiropyran) and the fluorophore (rhodamine B) in a size distribution between 98 and 140 nm and were characterized in terms of size, switching properties, and fluorescence efficiency by TEM, and UV\Vis and fluorescence spectroscopy.
Phosphofructokinase type 1 kinetics, isoform expression, and gene polymorphisms in cancer cells
Authors: Moreno‐Sánchez Rafael , Marín‐Hernández Alvaro , Gallardo‐Pérez Juan Carlos , Quezada Héctor , Encalada Rusely , Rodríguez‐Enríquez Sara , Saavedra Emma 
Published: December 2011

AbstractKinetic analysis of PFK‐1 from rodent AS‐30D, and human HeLa and MCF‐7 carcinomas revealed sigmoidal [fructose 6‐phosphate, Fru6P]‐rate curves with different Vm values when varying the allosteric activator fructose 2,6 bisphosphate (Fru2,6BP), AMP, Pi, NH, or K+. The rate equation that accurately predicted this behavior was the exclusive ligand binding concerted transition model together with non‐essential hyperbolic activation. PFK‐1 from rat liver and heart also exhibited the mixed cooperative‐hyperbolic kinetic behavior regarding activators. Lowering pH induced decreased affinity for Fru6P, Fru2,6BP, citrate, and ATP (as inhibitor); as well as decreased Vm and increased content of inactive (T) enzyme forms. High K+ prompted increased (Fru6P) or decreased (activators) affinities; increased Vm; and increased content of active (R) enzyme forms. mRNA expression analysis and nucleotide sequencing showed that the three PFK‐1 isoforms L, M, and C are transcribed in the three carcinomas. However, proteomic analysis indicated the predominant expression of L in liver, of M in heart and MCF‐7 cells, of L > M in AS‐30D cells, and of C in HeLa cells. PFK‐1M showed the highest affinities for F6P and citrate and the lowest for ATP (substrate) and F2,6BP; PFK‐1L showed the lowest affinity for F6P and the highest for F2,6BP; and PFK‐1C exhibited the highest affinity for ATP (substrate) and the lowest for citrate. Thus, the present work documents the kinetic signature of each PFK‐1 isoform, and facilitates the understanding of why this enzyme exerts significant or negligible glycolysis flux‐control in normal or cancer cells, respectively, and how it regulates the onset of the Pasteur effect. J. Cell. Biochem. 113: 1692–1703, 2012. © 2011 Wiley Periodicals, Inc.
Proteomic identification of PSF and p54(nrb) as topBP1‐interacting proteins
Authors: Kuhnert Anja , Schmidt Uta , Monajembashi Shamci , Franke Claudia , Schlott Bernhard , Grosse Frank , Greulich Karl Otto , Saluz Hans‐Peter , Hänel Frank 
Published: December 2011

AbstractTopBP1 is a BRCT domain‐rich protein that is structurally and functionally conserved throughout eukaryotic organisms. It is required for the initiation of DNA replication and for DNA repair and damage signalling. To further dissect its biological functions, we explored TopBP1‐interacting proteins by co‐immunoprecipitation assays and LC‐ESI‐MS‐analyses. As TopBP1 binding partners we identified p54(nrb) and PSF, and confirmed the physical interactions by GST pull‐down assays, co‐immunoprecipitations and by yeast two‐hybrid experiments. Recent evidence shows an involvement of p54(nrb) and PSF in DNA double‐strand break repair (DSB) and radioresistance. To get a first picture of the physiological significance of the interaction of TopBP1 with p54(nrb) and PSF we investigated in real time the spatiotemporal behaviour of the three proteins after laser microirradiation of living cells. Localisation of TopBP1 at damage sites was noticed as early as 5 s following damage induction, whereas p54(nrb) and PSF localised there after 20 s. Both p54(nrb) and PSF disappeared after 200 s while TopBP1 was retained at damage sites significantly longer suggesting different functions of the proteins during DSB recognition and repair. J. Cell. Biochem. 113: 1744–1753, 2012. © 2012 Wiley Periodicals, Inc.
Overexpression of 5‐lipoxygenase and its relation with cell proliferation and angiogenesis in 7,12‐dimethylbenz(α)anthracene‐induced rat mammary carcinogenesis
Authors: Chatterjee Mary , Das Subhadeep , Roy Kaushik , Chatterjee Malay 
Published: December 2011

AbstractThe present study was performed to investigate the critical role of 5‐lipoxygenase (5‐LOX) in 7,12‐dimethylbenz(α)anthracene (DMBA)‐induced rat mammary inflammation associated carcinogenesis. Female Sprague–Dawley rats at 50 days of age were treated with 7,12‐dimethylbenz(α)anthracene (DMBA; 0.5 mg/100 g body weight) by a single tail vein injection, followed by administration of zileuton (2000 mg/kg diet) from week 7 until the termination of the study at 31 wk. 5‐LOX protein expression, 5‐hydroxyeicosatetraenoic acid (5‐HETE), and leukotriene B4 (LTB4) production in rat mammary tissue were analyzed at 6, 12, and 24 wk post‐DMBA injection. Rate of cell proliferation was analyzed by bromodioxyuridine labeling index (BrdU‐LI). Microvessel density, level of VEGF, and MMP‐2 were also measured. DMBA induces inflammation in rat mammary gland as early as 6 wk. 5‐LOX is upregulated in DMBA treated rats right from 6 wk when compared with their normal counterparts. An overexpression of 5‐LOX is accompanied with increase in 5‐HETE, LTB4 production and high BrdU‐LI with an increase of two key angiogenic factors for tumorigenesis; MMP‐2 and VEGF. It was found that 5‐LOX specific inhibitor brought about substantial protection against DMBA‐induced mammary carcinogenesis. Histological findings showed substantial repair of hyperplastic lesions. There was a significant reduction in the rate of cell proliferation and expression of angiogenic factors, MMP‐2 and VEGF. 5‐LOX plays an important role in DMBA‐induced inflammation associated carcinogenesis via activation of MMP‐2 and VEGF. 5‐LOX expression can be considered as a critical event in controlling the process of mammary tumor development. © 2011 Wiley Periodicals, Inc.
Evaluation of combined bevacizumab plus irinotecan therapy in brain tumors using magnetic resonance imaging measures of relative cerebral blood volume
Authors: Pechman Kimberly R. , Donohoe Deborah L. , Bedekar Devyani P. , Kurpad Shekar N. , Schmainda Kathleen M. 
Published: December 2011

AbstractFrequently, bevacizumab is combined with chemotherapeutics such as irinotecan, motivated by studies showing improved clinical outcomes compared with historical controls. However, no systematic studies have been performed to determine if and how these drugs should be combined for optimal therapeutic response. The purpose of this study was to characterize the temporal combinations of bevacizumab and irinotecan by measuring the contrast‐agent enhanced tumor volumes and relative cerebral blood volume using dynamic susceptibility contrast imaging. The studies, performed in the U87 brain tumor model, show a vascular normalization window with bevacizumab monotherapy and are consistent with clinical indications of no additional benefit in the addition of irinotecan to bevacizumab therapy. Magn Reson Med, 2012. © 2011 Wiley Periodicals, Inc.
In vivo dopaminergic and behavioral responses to acute cocaine are altered in adenosine A2A receptor knockout mice
Authors: Wells Lisa , Opacka‐Juffry Jolanta , Fisher Donald , Ledent Catherine , Hourani Susanna , Kitchen Ian 
Published: December 2011

AbstractAdenosine, acting on adenosine A2A receptors (A2ARs), regulates addictive processes induced by drugs of abuse. This study investigates the role of A2A adenosine receptors in neurochemical and behavioral responses to an acute cocaine challenge. Changes in the extracellular levels of dopamine (DA) in the nucleus accumbens (NAc) of mice lacking A2A adenosine receptors and wild type (WT) littermates after an acute cocaine (20 mg/kg) administration were evaluated by in vivo microdialysis studies. Locomotor effects induced by cocaine were measured during the microdialysis procedure. Cocaine‐evoked increases in extracellular DA were not sustained in mice lacking A2ARs in comparison with wild‐type mice (P < 0.05). Cocaine administration significantly increased ambulatory activity in both genotypes. However, overall locomotor activity was further increased, whereas rest and small local movement measures were significantly attenuated in the A2AR knockout mice compared with WT littermates (P < 0.05). Our findings support an important role for adenosine A2AR in modulating the acute effects of cocaine, as demonstrated by the decrease in cocaine‐evoked dopaminergic transmission in the NAc. Furthermore, the results support an important antagonistic role of A2AR in vivo in regulating psychostimulant‐induced hyperlocomotion. Synapse, 2012. © 2011 Wiley Periodicals, Inc.
Multiple Lymphatic Basin Drainage from Cutaneous Melanoma as a Prognostic Factor
Authors: Piñero Antonio , de Torre Carlos , Martínez‐Escribano Jorge , Campillo Juana , Canteras Manuel , Nicolás Francisco 
Published: December 2011

AbstractBackgroundThere is some controversy in the literature regarding the possible prognostic value of cases of multiple lymphatic basin drainage (MLBD). The purpose of this work was to study the differences in prognosis depending on whether there is MLBD from primary cutaneous melanoma.MethodsWe conducted a cohort analysis from a prospective database, and 112 consecutive patients with cutaneous melanoma were included. Sentinel lymph node biopsy (SLNB) was done in all of them. MLBD was defined as the occurrence of two or more different nodal basins from the same lesion. The demographic and clinical data for cases with a single nodal drainage basin and MLBD were statistically compared using Fisher’s exact test, the χ2 test, or Mann–Whitney’s test according to the type of variables studied. Multivariate analysis also was performed on the disease‐free survival rate using logistic regression analysis. The distribution of disease‐free survival was determined using a Cox proportional risk model.ResultsOnly gender (27% men and 8% women; P = 0.01) and the localization of the primary tumor in the trunk (P < 0.001) were associated with the presence of MLBD. It also was observed that the cases with a high Breslow thickness or with MLBD were only associated with a worse disease‐free survival rate in cases with positive (P < 0.01 and P = 0.047, respectively) and negative (P < 0.011 and P = 0.019, respectively) SLNB.ConclusionsThis study suggests that both Breslow thickness and the presence of MLBD are statistically significant independent prognostic factors of disease‐free survival in patients with cutaneous melanoma.
Immunology in the clinic review series; focus on type 1 diabetes and viruses: enterovirus, thymus and type 1 diabetes pathogenesis
Authors: Jaïdane H , Sané F , Hiar R , Goffard A , Gharbi J , Geenen V , Hober D 
Published: December 2011

SummaryOTHER THEMES PUBLISHED IN THIS IMMUNOLOGY IN THE CLINIC REVIEW SERIESMetabolic diseases, host responses, cancer, autoinflammatory diseases, allergy.Thymus dysfunction, especially immune suppression, is frequently associated with various virus infections. Whether viruses may disturb the thymus function and play a role in the pathogenesis of autoimmune diseases is an open issue. Enteroviruses, especially Coxsackievirus B4 (CV-B4), have been largely suggested as potential inducers or aggravating factors of type 1 diabetes (T1D) pathogenesis in genetically predisposed individuals. Several pathogenic mechanisms of enterovirus-induced T1D have been suggested. One of these mechanisms is the impairment of central self-tolerance due to viral infections. Coxsackievirus-B4 is able to infect murine thymus in vitro and in vivo and to infect human thymus in vitro. Thymic epithelial cells and thymocytes are targets of infection with this virus, and several abnormalities, especially disturbance of maturation/differentiation processes, were observed. Altogether, these data suggest that CV-B infection of thymus may be involved in the pathogenesis of T1D. Further investigations are needed to explore this hypothesis.