bluuebunny/crossref_metadata_2025_split huggingface dataset:
A prediction rule for disease outcome in patients with undifferentiated arthritis using magnetic resonance imaging of the wrists and finger joints and serologic autoantibodies
Authors: Tamai Mami , Kawakami Atsushi , Uetani Masataka , Takao Shoichiro , Arima Kazuhiko , Iwamoto Naoki , Fujikawa Keita , Aramaki Toshiyuki , Kawashiri Shin‐YA , Ichinose Kunihiro , Kamachi Makoto , Nakamura Hideki , Origuchi Tomoki , Ida Hiroaki , Aoyagi Kiyoshi , Eguchi Katsumi 
Published: May 2009

AbstractObjectiveTo evaluate whether magnetic resonance imaging (MRI) of the wrists and finger joints and an analysis of serologic autoantibodies are clinically meaningful for the subsequent development of rheumatoid arthritis (RA) in patients with undifferentiated arthritis (UA).MethodsA total of 129 patients with UA, a disease status formally confirmed by a rheumatologist over a period of at least 1 year, were included. Gadolinium‐diethylenetriamine–enhanced MRI of both wrists and finger joints and serologic variables were examined upon admission to our Early Arthritis Clinic at Nagasaki University. After a prospective followup of 1 year, a predictive value for the development of RA was determined for each patient.ResultsThe subjects were evaluated for their positive or negative status with respect to 3 objective measures at study entry: anti–cyclic citrullinated peptide (anti‐CCP) antibodies and/or IgM‐rheumatoid factor, MRI‐proven symmetric synovitis, and MRI‐proven bone edema and/or bone erosion. The patients who were positive for at least 2 of these measures progressed to RA at 1 year with a 79.7% positive predictive value (PPV), 63.0% negative predictive value, 75.9% specificity, 68.0% sensitivity, and 71.3% accuracy. Furthermore, in 22 UA patients positive for both anti‐CCP antibodies and MRI‐proven bone edema who were considered to have progressed to RA at 1 year, the PPV was increased to 100%. A close correlation was found between the present rule and that established in the Leiden Early Arthritis Cohort.ConclusionMRI‐proven early joint damage in conjunction with serologic autoantibodies is efficient in predicting progression from UA to RA. This method can be used to identify patients who would benefit from early treatment with disease‐modifying antirheumatic drugs.
Cobalt‐Catalyzed Isomerization of 1‐Alkenes to (E)‐2‐Alkenes with Dimethylphenylsilylmethylmagnesium Chloride and Its Application to the Stereoselective Synthesis of (E)‐Alkenylsilanes
Authors: Kobayashi Tsuneyuki , Yorimitsu Hideki , Oshima Koichiro 
Published: May 2009

Abstract“Co”axing selectivity into isomerization: Treatment of 1‐alkenes with dimethylphenylsilylmethylmagnesium chloride in the presence of a cobalt‐NHC complex in dioxane at 50 °C or higher provides the corresponding (E)‐2‐alkenes selectively. The isomerization is applicable to the stereoselective synthesis of (E)‐crotylsilanes and (E)‐1‐propenylsilanes from the corresponding homoallylsilanes and allylsilanes, respectively.magnified imageTreatment of 1‐alkenes with dimethylphenylsilylmethylmagnesium chloride in the presence of a cobalt‐NHC complex in dioxane at 50 °C or higher provides the corresponding (E)‐2‐alkenes selectively. The isomerization is applicable to the stereoselective synthesis of (E)‐crotylsilanes and (E)‐1‐propenylsilanes from the corresponding homoallylsilanes and allylsilanes, respectively.
Reactive oxygen species formation is not enhanced by exposure to UMTS 1950 MHz radiation and co‐exposure to ferrous ions in Jurkat cells
Authors: Brescia Francesca , Sarti Maurizio , Massa Rita , Calabrese Maria L. , Sannino Anna , Scarfì Maria R. 
Published: May 2009

AbstractThis study was designed to assess if radiofrequency (RF) radiation induces oxidative stress in cultured mammalian cells when given alone or in combination with ferrous ions (FeSO4). For this purpose the production of reactive oxygen species (ROS) was measured by flow cytometry in human lymphoblastoid cells exposed to 1950 MHz signal used by the third generation wireless technology of the Universal Mobile Telecommunication System (UMTS) at Specific Absorption Rate of 0.5 and 2.0 W/kg. Short (5–60 min) or long (24 h) duration exposures were carried out in a waveguide system under strictly controlled conditions of both dosimetry and environment. Cell viability was also measured after 24 h RF exposure using the Resazurin and Neutral Red assays. Several co‐exposure protocols were applied to test if RF radiation is able to alter ROS formation induced by FeSO4 (RF given before or concurrently to FeSO4). The results obtained indicate that non‐thermal RF exposures do not increase spontaneous ROS formation in any of the experimental conditions investigated. Consistent with the lack of ROS production, no change in cell viability was observed in Jurkat cells exposed to RF radiation for 24 h. Similar results were obtained when co‐exposures were considered: combined exposures to RF radiation and FeSO4 did not increase ROS formation induced by the chemical treatment alone. In contrast, in cultures treated with FeSO4 as positive control, a dose‐dependent increase in ROS formation was recorded, validating the sensitivity of the method employed. Bioelectromagnetics 30:525–535, 2009. © 2009 Wiley‐Liss, Inc.
Effect of radiofrequency electromagnetic field exposure on in vitro models of neurodegenerative disease
Authors: Del Vecchio Giovanna , Giuliani Alessandro , Fernandez Mercedes , Mesirca Pietro , Bersani Ferdinando , Pinto Rosanna , Ardoino Lucia , Lovisolo Giorgio A. , Giardino Luciana , Calzà Laura 
Published: May 2009

AbstractIn this work we tested viability, proliferation, and vulnerability of neural cells, after continuous radiofrequency (RF) electromagnetic fields exposure (global system for mobile telecommunications (GSM) modulated 900 MHz signal at a specific absorption rate (SAR) of 1 W/kg and maximum duration 144 h) generated by transverse electromagnetic cells. We used two cellular systems, SN56 cholinergic for example, SN56 cholinergic cell line and rat primary cortical neurons, and well‐known neurotoxic challenges, such as glutamate, 25‐35AA beta‐amyloid, and hydrogen peroxide. Exposure to RF did not change viability/proliferation rate of the SN56 cholinergic cells or viability of cortical neurons. Co‐exposure to RF exacerbated neurotoxic effect of hydrogen peroxide in SN56, but not in primary cortical neurons, whereas no cooperative effects of RF with glutamate and 25‐35AA beta‐amyloid were found. These data suggest that only under particular circumstances exposure to GSM modulated, 900 MHz signal act as a co‐stressor for oxidative damage of neural cells. Bioelectromagnetics 30:564–572, 2009. © 2009 Wiley‐Liss, Inc.
Large gradient high magnetic field affects the association of MACF1 with actin and microtubule cytoskeleton
Authors: Qian Ai‐Rong , Hu Li‐Fang , Gao Xiang , Zhang Wei , Di Sheng‐Meng , Tian Zong‐Cheng , Yang Peng‐Fei , Yin Da‐Chuan , Weng Yuan‐Yuan , Shang Peng 
Published: May 2009

AbstractThe intense inhomogeneous magnetic fields acting on the diamagnetic materials naturally present in cells can generate strong magnetic forces. We have developed a superconducting magnet platform with large gradient high magnetic field (LG‐HMF), which can produce three magnetic force fields of −1360, 0, and 1312 T2/m, and three corresponding apparent gravity levels, namely 0, 1, and 2‐g for diamagnetic materials. In this study, the effects of different magnetic force fields on osteoblast‐like cells (MG‐63 and MC3T3‐E1) viability, microtubule actin crosslinking factor 1 (MACF1) expression and its association with cytoskeleton were investigated. Results showed that cell viability increased to different degrees after exposure to 0 or 1‐g conditions for 24 h, but it decreased by about 30% under 2‐g conditions compared with control conditions. An increase in MACF1 expression at the RNA or protein level was observed in osteoblast‐like cells under the magnetic force field of −1360 T2/m (0‐g) relative to 1312 T2/m (2‐g). Under control conditions, anti‐MACF1 staining was scattered in the cytoplasm and partially colocalized with actin filaments (AFs) or microtubules (MTs) in the majority of osteoblast‐like cells. Under 0‐g conditions, MACF1 labeling was concentrated at perinuclear region and colocalization was not apparent. The patterns of anti‐MACF1 labeling on MTs varied with MTs' changing under LG‐HMF environment. In conclusion, LG‐HMF affects osteoblast‐like cell viability, MACF1 distribution, expression, and its association with cytoskeleton to some extent. Bioelectromagnetics 30:545–555, 2009. © 2009 Wiley‐Liss, Inc.
Metallothionein‐I+II in neuroprotection
Authors: Pedersen Mie Ø. , Jensen Rikke , Pedersen Dan S. , Skjolding Anders D. , Hempel Casper , Maretty Lasse , Penkowa Milena 
Published: May 2009

AbstractMetallothionein (MT)‐I+II synthesis is induced in the central nervous system (CNS) in response to practically any pathogen or disorder, where it is increased mainly in reactive glia. MT‐I+II are involved in host defence reactions and neuroprotection during neuropathological conditions, in which MT‐I+II decrease inflammation and secondary tissue damage (oxidative stress, neurodegeneration, and apoptosis) and promote post‐injury repair and regeneration (angiogenesis, neurogenesis, neuronal sprouting and tissue remodelling). Intracellularly the molecular MT‐I+II actions involve metal ion control and scavenging of reactive oxygen species (ROS) leading to cellular redox control. By regulating metal ions, MT‐I+II can control metal‐containing transcription factors, zinc‐finger proteins and p53. However, the neuroprotective functions of MT‐I+II also involve an extracellular component. MT‐I+II protects the neurons by signal transduction through the low‐density lipoprotein family of receptors on the cell surface involving lipoprotein receptor‐1 (LRP1) and megalin (LRP2). In this review we discuss the newest data on cerebral MT‐I+II functions following brain injury and experimental autoimmune encephalomyelitis. © 2009 International Union of Biochemistry and Molecular Biology, Inc.
Zn2+‐transporter‐8: A dual role in diabetes
Authors: Chistiakov Dimitry A. , Voronova Natalia V. 
Published: May 2009

AbstractDiabetes mellitus arises from defects in insulin secretion or action, or both. In pancreatic islets, insulin production is linked with zinc transport mediated by zinc transporter ZnT‐8, a product of the SLC30A8 gene. Therefore, altered activity of ZnT‐8 is expected to be associated with impaired glucose‐induced insulin response and promote progression from glucose intolerance to diabetes. Recent findings do emerge with a role of SLC30A8 in diabetes. Genome‐wide association scans for type 2 diabetes (T2D) susceptibility loci revealed and then replicated a highly significant association between the R allele of the R325W variant of SLC30A8 (marker rs13266634) and susceptibility to T2D in Caucasians. A role of ZnT‐8 as a new major self‐antigenic determinant in type 1 diabetes (T1D) was found. Marker rs13266634 was also shown to modulate anti‐ZnT‐8 self‐antibody specificity in islet autoimmunity. Hence, these findings suggest for a dual role of SLC30A8 in diabetes, which is consisted in conferring genetic susceptibility to T2D and being a major islet self‐antigen in T1D as well. Here we characterize an emerging role of ZnT‐8 in diabetes and discuss potential mechanisms of its involvement in the etiology of both forms of diabetes. © 2009 International Union of Biochemistry and Molecular Biology, Inc.
Asymmetric division of Hansenula polymorpha reflected by a drop of light scatter intensity measured in batch microtiter plate cultivations at phosphate limitation
Authors: Kottmeier Kirsten , Weber Jost , Müller Carsten , Bley Thomas , Büchs Jochen 
Published: May 2009

AbstractHansenula polymorpha RB11 pC10‐FMD (PFMD− GFP) (FMD promoter gfp gene) was simultaneously cultivated in the Respiration Activity Monitoring System (RAMOS) and in the microtiter plate cultivation system “BioLector” under phosphate limitation. The light scatter signal of the BioLector, for the determination of the biomass concentration in the wells, shows a significant decrease with the onset of the phosphate limitation until a stationary level is reached. At lower initial phosphate concentration this effect is more pronounced and longer time is required until the stationary level of the scattered light is achieved. The oxygen transfer rate signal of the RAMOS and the light scatter signal of the BioLector correlate with respect to the points of time where the maxima and the stationary levels of the courses are reached. In order to understand the effect causing this light scatter behavior, the forward and side scatter properties were investigated off line by flow cytometry. The decay in the light scatter of the BioLector seems to correlate with the formation of two subpopulations of different scatter intensities detected by a flow cytometer. With ongoing cultivation the fraction of cells possessing higher light scattering properties decreases until only a population of lower light scattering properties exists. The rate of transition of the yeast from one subpopulation to the other appears to be correlated with the rate of decrease in the BioLector light scatter signal. The formation of the subpopulations may be caused by an increased asymmetry in the cell cycle due to phosphate limitation. Biotechnol. Bioeng. 2009; 104: 554–561 © 2009 Wiley Periodicals, Inc.
Determination of intrinsic monod kinetic parameters for two heterotrophic tetrachloroethene (PCE)‐respiring strains and insight into their application
Authors: Huang Deyang , Becker Jennifer G. 
Published: May 2009

AbstractA complete set of mathematically identifiable and meaningful kinetic parameters estimates is needed to accurately describe the activity of individual populations that dehalorespire tetrachloroethene (PCE) and other chlorinated ethenes. These data may be difficult to extract from the literature because kinetic parameter estimates obtained using mixed cultures may reflect the activity of multiple dehalorespiring populations, while those obtained at low initial substrate‐to‐biomass ratios (S0/X0) are influenced by culture history and are generally not relevant to other systems. This study focused on estimation of electron donor and acceptor utilization kinetic parameters for the heterotrophic dehalorespirers Desulfuromonas michiganensis strain BB1 and Desulfitobacterium sp. strain PCE1. Electron acceptor utilization kinetic parameters that are identifiable and independent of culture history, i.e., intrinsic, could be estimated at S0/X0 ≥ 10, with both concentrations expressed as chemical oxygen demand (COD). However, the parameter estimates did not accurately describe dechlorination kinetics at lower S0/X0 ratios. The maximum specific substrate utilization rates (qmax) and half‐saturation constants (KS) for PCE and trichloroethene (TCE) estimated for the two heterotrophic strains are higher than the values reported for Dehalococcoides cultures. These results suggest that the natural niche of Dehalococcoides strains that can metabolize a range of chlorinated ethenes may be to respire dichloroethene and vinyl chloride produced by Desulfuromonas and Desulfitobacterium strains or other populations that dechlorinate PCE and TCE at faster rates. Few data exist on the electron donor utilization kinetics of heterotrophic dehalorespirers. The results of this study suggest that Desulfuromonas and Desulfitobacterium strains should be able to compete for organic electron donors with other heterotrophic populations in the subsurface. Biotechnol. Bioeng. 2009; 104: 301–311 © 2009 Wiley Periodicals, Inc.
A unified shape editing framework based on tetrahedral control mesh
Authors: Zhao Yong , Liu Xinguo , Xiao Chunxia , Peng Qunsheng 
Published: May 2009

AbstractIt is a fundamental but challenging problem to efficiently edit complex 3D objects. By embedding the input models into coarse tetrahedral control meshes, this paper develops a unified framework to discuss two useful editing operations: interactive deformation and deformation transfer. First, a new rigidity energy is proposed to make the tetrahedral control mesh deform as rigidly as possible, which yields intuitive detail and volume preservation even under large deformations. And an error‐driven refinement approach is presented to further improve the deformation result. Then, based on this deformation scheme, a volumetric correspondence method is introduced to perform the deformation transfer task between the tetrahedral control meshes of the source and target models, which greatly lessens the burden of the user. Experimental results show our algorithm is effective, easy to control, supports various shape representations, and well transfers deformations between non‐homeomorphous models. Copyright © 2009 John Wiley & Sons, Ltd.
Competitive motion synthesis based on hybrid control
Authors: Liang Zhang , Xiao Jun , Zhuang Yueting , Chen Cheng 
Published: May 2009

AbstractWe propose a simple and effective framework to deal with the problem of synthesizing interactive and competitive motions while reflecting the interactions. Two nontrivial issues are addressed in synthesizing two‐character motions in competitive environment: how to reveal the embedded routines while keeping visual reality and how to build interactive models based on singly captured motions. To solve these issues, we employ a hierarchical framework: the finite state machine (FSM) controls the state transition in the higher layer, and the hybrid approach controls the action selection in the lower layer. The proposed approach contains two folds: first, a rule‐based control scheme is proposed to simulate routine steps based on statistical analysis. Second, the interactive models are designed for simulating dense interactions between two players. The Relevance Vector Machine (RVM) algorithm is adopted to select attack styles and coupled with motion transition graph to determine combination blows. Here we apply the proposed framework of hybrid paradigm to boxing sport as an example. Copyright © 2009 John Wiley & Sons, Ltd.
Cytotoxic Cytochalasin Metabolites of Endophytic Endothia gyrosa
Authors: Xu Shu , Ge Hui Ming , Song Yong Chun , Shen Yao , Ding Hui , Tan Ren Xiang 
Published: May 2009

Abstractmagnified imageIn addition to the known metabolites cytochalasin H (1), cytochalasin J (2), and epoxycytochalasin H (3), two new 10‐phenyl‐(11)‐cytochalasans, named cytochalasin Z10 and Z11 (4 and 5, resp.) were isolated from the solid substrate culture of Endothia gyrosa IFB‐E023, an endophytic fungus residing inside the healthy leaf of Vatica mangachapo (Dipterocarpaceae). The structure determination of 4 and 5 was accomplished through correlative analyses of their spectral data (UV, ESI‐MS, IR, 1H‐ and 13C‐NMR, COSY, NOESY, HMQC, and HMBC). Metabolites 1–5 were demonstrated to be substantially cytotoxic to the human leukaemia K562 cell line with the IC50 values of 10.1, 1.5, 24.5, 28.3, and 24.4 μM, respectively, which are comparable to that of 5‐fluorouracil (33.0 μM), co‐assayed as the positive reference.
A 1 : 2 Copper(II)–Tripeptide Complex for DNA Binding and Cleavage Agent under Physiological Conditions
Authors: Reddy Pulimamidi R. , Manjula Pallerla , Chakraborty Tushar K. , Samanta Rajarshi 
Published: May 2009

Abstractmagnified imageA 1 : 2 copper–tripeptide complex, [CuII(Boc‐His‐Gly‐His‐OMe)2]2+, was synthesized and structurally characterized. The absorption band at 577 nm suggests a square‐planar geometry around CuII. The DNA‐binding and DNA‐cleavage properties of the CuII complex were investigated. The complex binds to calf thymus DNA (CT DNA) in an intercalative fashion and cleaves plasmid pUC‐19 DNA hydrolytically at micromolar concentrations under physiological conditions. The intrinsic binding constant (Kb=1.2×102 M−1) for the binding of Cu–tripeptide complex with DNA suggests that this complex is suitable for rapid diffusion on the pharmacological time scale.
Cyclen‐Based Side‐Chain Homopolymer Self‐Assembly with Plasmid DNA: Protection of DNA from Enzymatic Degradation
Authors: Li Kun , Wang Na , Yang Fan , Zhang Zhong‐Wei , Zhou Li‐Hong , Chen Shan‐Yong , Lin Hong‐Hui , Tian Yun‐Fei , Yu Xiao‐Qi 
Published: May 2009

Abstractmagnified imageIn this study, a 1,4,7,10‐tetraazacyclododecane (cyclen)‐based side‐chain homopolymer was developed for the first time; this polymer can self‐assembly with plasmid DNA to form polyelectrolyte complexes (polyplex), which can protect DNA from enzymatic degradation. Moreover, the polyplex can disassembly and release free DNA when NaCl solution is added to this system. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) are used for imaging of the surface structure of the polyplex, and results indicated that the polyplex structures respond to the polymer concentration. Circular dichroism (CD) spectrum suggested that the DNA configuration in the polyplex was retained.
Biological Evaluation of ent‐Narciclasine, ent‐Lycoricidine, and Certain Enantiomerically‐Related Congeners
Authors: Matveenko Maria , Banwell Martin G. , Joffe Max , Wan Soosan , Fantino Emmanuelle 
Published: May 2009

Abstractmagnified imageThe non‐natural enantiomeric forms of narciclasine and lycoricidine ((−)‐1 and (−)‐2, respectively), as well as congeners 3–6 are available through chemoenzymatic synthesis. Accordingly, they have now been tested for their cytotoxic effects in a 13‐member human cancer cell‐line panel and found to be only weakly active. In contrast, an authentic sample of the natural enantiomeric form of narciclasine ((+)‐1) was found to be highly active in the same screens.
The Biochemistry of Drug Metabolism – An Introduction
Authors: Testa Bernard , Krämer Stefanie D. 
Published: May 2009

Abstractmagnified imageThis review continues a general presentation of the metabolism of drugs and other xenobiotics begun in five recent issues ofChemistry & Biodiversity.The presentPartis dedicated to the pharmacological and toxicological consequences of drug and xenobiotic metabolism. In other words, the key concepts here are activationvs.deactivation, toxificationvs.detoxification, and their interplay. These concepts are illustrated with a number of medicinally, toxicologically, and environmentally relevant examples. But, far from being concerned only with individual cases, the review is based on broad classifications, global rationalizations, and synthetic hypotheses.
Synthesis of 7‐Aza‐ and 7‐Thiasphingosines, and Evaluation of Their Interaction with Sphingosine Kinases and with T‐Cells
Authors: Mathew Thresen , Billaud Célia , Billich Andreas , Cavallari Marco , Nussbaumer Peter , De Libero Gennaro , Vasella Andrea 
Published: May 2009

Abstractmagnified imageThe synthesis of 7‐oxasphingosine (3) and 7‐oxaceramide (4) was improved by starting from the 4‐methoxybenzyl‐protected d‐galactal 9. The sphingosine analogues 5–7 and 24 were synthesized via the azido alcohol 13. The 7‐thiasphingosine 5 is a poorer substrate for both isoforms of sphingosine kinase (SPHK) than sphingosine, but showed a slight preference for SPHK2. The sulfone 6 and the 7‐aza compounds 7 and 24 were not phosphorylated by either SPHK1 or SPHK2, and none of 5–7 and 24 activated invariant natural killer T (iNKT) cell clones when presented by human CD1d‐transfected antigen‐presenting cells (APC) or by plate‐bound human CD1d. Only 7 and 24 associated with plate‐bound recombinant CD1d prevented stimulation of iNKT cells by α‐galactosylceramide (α‐GalCer).