bluuebunny/crossref_metadata_2025_split huggingface dataset:
Known pathogenic gene variants and new candidates detected in sudden unexpected infant death using whole genome sequencing
Authors: Bard Angela M. , Clark Lindsay V. , Cosgun Erdal , Aldinger Kimberly A. , Timms Andrew , Quina Lely A. , Ferres Juan M. Lavista , Jardine David , Haas Elisabeth A. , Becker Tatiana M. , Pagan Chelsea M. , Santani Avni , Martinez Diego , Barua Soumitra , McNutt Zakkary , Nesbitt Addie , Mitchell Edwin A. , Ramirez Jan‐Marino 
Published: June 2024

AbstractThe purpose of this study is to gain insights into potential genetic factors contributing to the infant's vulnerability to Sudden Unexpected Infant Death (SUID). Whole Genome Sequencing (WGS) was performed on 144 infants that succumbed to SUID, and 573 healthy adults. Variants were filtered by gnomAD allele frequencies and predictions of functional consequences. Variants of interest were identified in 88 genes, in 64.6% of our cohort. Seventy‐three of these have been previously associated with SIDS/SUID/SUDP. Forty‐three can be characterized as cardiac genes and are related to cardiomyopathies, arrhythmias, and other conditions. Variants in 22 genes were associated with neurologic functions. Variants were also found in 13 genes reported to be pathogenic for various systemic disorders and in two genes associated with immunological function. Variants in eight genes are implicated in the response to hypoxia and the regulation of reactive oxygen species (ROS) and have not been previously described in SIDS/SUID/SUDP. Seventy‐two infants met the triple risk hypothesis criteria. Our study confirms and further expands the list of genetic variants associated with SUID. The abundance of genes associated with heart disease and the discovery of variants associated with the redox metabolism have important mechanistic implications for the pathophysiology of SUID.
Assessing the quality of artificial intelligence–generated patient counseling for rhinosinusitis
Authors: Hill Gregory S. , Fischer Jakob L. , Watson Nora L. , Riley Charles A. , Tolisano Anthony M. 
Published: June 2024

Key points
GPT‐4 generated moderate quality information in response to questions regarding sinusitis and surgery.
GPT‐4 generated significantly higher quality responses to questions regarding treatment of sinusitis.
Future studies exploring quality of GPT responses should seek to limit bias and use validated instruments.
Leguminous ferritin, a natural protein for iron supplementation, Pickering emulsion formation and encapsulation of bioactive molecules
Authors: Hang Jiayi , Chu Yifu , Chen Lingyun 
Published: June 2024

AbstractFerritin is a naturally occurring iron storage protein. Leguminous ferritins exhibit unique structural features, including diverse subunit composition and an extension peptide, which contribute to superior thermal stability compared to animal ferritins. The high iron content, remarkable effectiveness, low risk of oxidative damage and thermal stability make the leguminous ferritin an attractive candidate for iron supplementation. Moreover, apoferritin is an excellent nanosized carrier for encapsulating bioactive compounds due to its inherent inner cavity, water solubility, biocompatibility, and reversible self‐assembly behavior. However, the harsh condition during encapsulation by unmodified ferritins may cause damage to sensitive bioactive compounds. Thus, different processing methods are employed to alter the leguminous ferritin structures, including chemical, enzymatic, mild heat treatments, and nonthermal processing to achieve gentler encapsulation conditions for a wide range of bioactive compounds. Another challenge is to improve the stability of leguminous ferritin to withstand gastric digestion. The degradation of ferritin by proteases may lead to premature release of bioactive compounds. Recent works demonstrated that certain phenolic compounds such as proanthocyanidin‐induced protein association, thereby enhancing digestive stability of ferritins, leading to a sustained release and a potentially greater bioavailability of bioactive compounds. Leguminous ferritin also has the potential to serve as a stabilizer for the Pickering emulsion, where the hydrophilic and hydrophobic compounds can be encapsulated in the ferritin nanocages and oil phase, respectively. The release and absorption of bioactive compounds in encapsulates and emulsions will need to be further demonstrated through in vivo studies.
Synthesis of triphenol‐diamine‐type hyperbranched benzoxazine and improvement of thermal properties and toughness of typical benzoxazines
Authors: Hu Ling‐Xiao , Wang Zhi‐Cheng , Guo Zhi‐Yi , Liu Tao , Yuan Zhi‐Gang , Derradji Mehdi , Liu Wen‐Bin , Wang Jun 
Published: June 2024

AbstractA series of triphenol‐diamine‐type hyperbranched benzoxazines are synthesized by using polyformaldehyde, 1,1,1‐tri(4‐hydroxyphenyl) ethane, and primary amines such as p‐phenylenediamine, 4,4′‐diaminodiphenylmethane, polyetheramine, and bifuranocyclic diamine via Mannich condensation. Then, copolymers with bifunctional benzoxazine (BA‐a) are prepared to obtain superior properties. The polymerization behavior of the copolymers examined using a differential scanning calorimeter and a Fourier transform infrared indicates that the introduction of hyperbranched benzoxazines facilitates the benzoxazine curing reaction, decreasing the onset and peak temperatures of the curing process. Thermogravimetric analysis results suggest the cured hyperbranched benzoxazine demonstrates good thermal stability and can improve the heat resistance of benzoxazine. In addition, dynamic mechanical analysis suggests the glass transition temperature of the copolymers with BA‐a was increased after copolymerization, thus making all the copolymers obtain higher service temperatures. The test results from the universal testing machine and the fracture morphologies of copolymers indicate the hyperbranched benzoxazines with branched structures cause dendritic folds to appear on the surface of copolymers upon polymerization, preventing rapid cracking and spreading of the thermoset resin by dissipating more impact energy through this irregular dendritic appearance, thus obtaining strength and toughness superior to that of BA‐a resins.
Target repurposing unravels avermectins and derivatives as novel antibiotics inhibiting energy‐coupling factor transporters (ECFTs)
Authors: Haupenthal Jörg , Rafehi Muhammad , Kany Andreas M. , Lespine Anne , Stefan Katja , Hirsch Anna K. H. , Stefan Sven Marcel 
Published: June 2024

AbstractEnergy‐coupling factor transporters (ECFTs) are membrane‐bound ATP‐binding cassette (ABC) transporters in prokaryotes that are found in pathogens against which novel antibiotics are urgently needed. To date, just 54 inhibitors of three molecular‐structural classes with mostly weak inhibitory activity are known. Target repurposing is a strategy that transfers knowledge gained from a well‐studied protein family to under‐studied targets of phylogenetic relation. Forty‐eight human ABC transporters are known that may harbor structural motifs similar to ECFTs to which particularly multitarget compounds may bind. We assessed 31 multitarget compounds which together target the entire druggable human ABC transporter proteome against ECFTs, of which nine showed inhibitory activity (hit rate 29.0%) and four demonstrated moderate to strong inhibition of an ECFT (IC50 values between 4.28 and 50.2 µM) as well as antibacterial activity against ECFT‐expressing Streptococcus pneumoniae. Here, ivermectin was the most potent candidate (MIC95: 22.8 µM), and analysis of five ivermectin derivatives revealed moxidectin as one of the most potent ECFT‐targeting antibacterial agents (IC50: 2.23 µM; MIC95: 2.91 µM). Distinct molecular‐structural features of avermectins and derivatives as well as the differential biological response of the hit compounds in general provided first indications with respect to the structure–activity relationships and mode of action, respectively.
Two‐ and Three‐Spin Hybrid Inorganic‐Organic [2]Rotaxanes Containing Metallated Salen Groups
Authors: Bennett Tom S. , Lockyer Selena J. , Asthana Deepak , Whitehead George F. S. , Vitorica‐Yrezabal Inigo J. , Timco Grigore A. , Winpenny Richard E. P. , McInnes Eric J. L. 
Published: June 2024

AbstractMono‐ and bis‐salen functionalised [2]rotaxanes have been synthesised from the esterification of [2]rotaxanes containing phenol‐terminated threads (salen=N,N′‐bis(salicylidene)ethylenediamine). The [2]rotaxanes have general formula [RH][Cr7NiF8(O2CtBu)16], where [RH]+ is a thread with a central secondary ammonium site that templates a [Cr7NiF8(O2CtBu)16]− ring. The threads are terminated at one or both ends by carboxylic acid functionalised salen groups. The {M(salen)} groups can be free‐base [M=(H+)2] or metallated [M=Cu2+, Ni2+, (VO)2+]. The [2]rotaxanes have been characterised by single crystal XRD and solid‐ and solution‐state EPR spectroscopy. Where two paramagnetic M ions are involved [M=Cu2+ and/or (VO)2+] the [2]rotaxanes contain three electron spin S=
centres, since the {Cr7Ni} ring has an S=
ground state which is well isolated at low temperatures. These three‐spin [2]rotaxanes have been characterised in solution by pulsed dipolar EPR spectroscopies (DEER, also known as PELDOR, and RIDME). The M⋅⋅⋅M and M⋅⋅⋅{Cr7Ni} interactions measured are consistent with dipolar interactions and also with the distances from single crystal XRD. The authors declare no competing financial interest.
Harnessing the Benefits of PEDOT : PSS Conductive Coating for Prolonged Cycle Life of Copper Hexacyanoferrate in Aqueous Zinc‐Ion Batteries
Authors: Baghodrat Mohsen , Glenneberg Jens , Zampardi Giorgia , La Mantia Fabio 
Published: June 2024

AbstractThe utilization of copper hexacyanoferrate (CuHCF) as positive electrode material in aqueous zinc‐ion batteries (ZIBs) has gained significant attention due to its efficient (de−)intercalation of Zn2+ ions, cost‐effective synthesis, low toxicity, and high working potential. One approach to improve its electrochemical performance is to coat the CuHCF particles with conductive polymers, such as poly(3,4‐ethylenedioxythiophene): polystyrene sulfonate (PEDOT : PSS). In this study, we investigated the impact of the PEDOT : PSS as a coating on the electrochemical behavior and the cycle life of CuHCF for aqueous ZIB applications. Galvanostatic cycling performed at a current rate of 1 C relevant for the stationary application of the CuHCF/PEDOT : PSS electrodes having high mass loadings (10 mg cm−2 of active material) revealed significantly longer cycle life while maintaining a high Coulombic efficiency (≥ 99.5 %). The longest cycle life was achieved with CuHCF coated using a 4.5 wt. % PEDOT : PSS aqueous coating dispersion. These findings demonstrate the potential of conductive polymer coatings as a practical approach to enhance the electrochemical performance of positive electrode materials in aqueous Zinc‐ion batteries.
Adjusting luminescence properties of ZnAl2O4:Mn2+(Mn4+), Li+ phosphors through cation substitution
Authors: Sun Dandan , Zeng Xiaoling , Yu Ying , Fu Yanhua , Yu Lixin 
Published: June 2024

AbstractZnAl2O4 with a typical spinel structure is highly expected to be a novel rare‐earth‐free ion‐activated oxide phosphor with red emission, which holds high actual meaning for advancing phosphor‐converted light‐emitting diode (pc‐LED) lighting. Among the rare‐earth‐free activators, Mn4+ ions have emerged as one of the most promising activators. Considering the price advantage of MnCO3 generating Mn2+ ions and the charge compensation effect potentially obtaining Mn4+ ions from Mn2+ ions, this research delves into a collection of ZnAl2O4:Mn2+(Mn4+), x Li+ (x = 0%–40%) phosphors with Li+ as co‐dopant and MnCO3 as Mn2+ dopant source prepared by a high temperature solid‐state reaction method. The lattice structure was investigated using X‐ray diffraction (XRD), photoluminescence (PL), and photoluminescence excitation (PLE) spectroscopy. Results suggest a relatively high probability of Li+ ions occupying Zn2+ lattice sites. Furthermore, Li+ ion doping was assuredly found to facilitate the oxidization of Mn2+ to Mn4+, leading to a shift of luminescence peak from 516 to 656 nm. An intriguing phenomenon that the emission color changed with the Li+ doping content was also observed. Meanwhile, the luminescence intensity and quantum yield (QY) at different temperatures, as well as the relevant thermal quenching mechanism, were determined and elucidated detailedly.
All‐in‐one platform: Versatile, Easy, and User‐friendly System (VEUS) based on automated and expert‐independent antibody immobilization and immunoassay by utilizing customized movement of magnetic particles
Authors: Yoon Jinsik , Kim Jiyeong , Lim Sujeong , Choi Heelak , Bae Junghyun , Kim Kibeom , Song Suk‐Heung , Cho Yoo‐Bok , Park Wook , Jung Yong‐Gyun 
Published: June 2024

AbstractThe ELISA is the most worldwide method for immunoassay. However, the ELISA is losing ground due to low reproducibility of manual experimental processes in both R&D and IVD areas. An automated platform is a good solution, but there are still limitations owning to extremely high cost and requiring large space to set up especially for a small size laboratory. Here, we present a novel all‐in‐one platform called “VEUS” settable on the laboratory table that offers comprehensive automation of the entire multiplex immunoassay process by exploiting antibody conjugated magnetic particles, quality control and then immunoanalytical reaction, thereby enhancing detection sensitivity and high reproducibility. As a proof of concept, the system exhibits a sensitive LOD of 0.6 and 3.1 pg mL−1 within 1 h run, comparable precision that of molecular diagnostic systems based on PCR method, enabling rapid multiplex diagnosis of Influenza A, Influenza B, and COVID‐19 viruses with similar symptoms. Through automation by the all‐in‐one system, it can be used by novice users, something innovative for immunoassays, relying heavily on user experience. Furthermore, it can contribute to streamline entire immunoassay processes of diverse biomarkers with high reproducibility and convenience in laboratories.
A machine learning‐based approach for improving plasmid DNA production in Escherichia coli fed‐batch fermentations
Authors: Xu Zhixian , Zhu Xiaofeng , Mohsin Ali , Guo Jianfei , Zhuang Yingping , Chu Ju , Guo Meijin , Wang Guan 
Published: June 2024

AbstractArtificial Intelligence (AI) technology is spearheading a new industrial revolution, which provides ample opportunities for the transformational development of traditional fermentation processes. During plasmid fermentation, traditional subjective process control leads to highly unstable plasmid yields. In this study, a multi‐parameter correlation analysis was first performed to discover a dynamic metabolic balance among the oxygen uptake rate, temperature, and plasmid yield, whilst revealing the heating rate and timing as the most important optimization factor for balanced cell growth and plasmid production. Then, based on the acquired on‐line parameters as well as outputs of kinetic models constructed for describing process dynamics of biomass concentration, plasmid yield, and substrate concentration, a machine learning (ML) model with Random Forest (RF) as the best machine learning algorithm was established to predict the optimal heating strategy. Finally, the highest plasmid yield and specific productivity of 1167.74 mg L−1 and 8.87 mg L−1/OD600 were achieved with the optimal heating strategy predicted by the RF model in the 50 L bioreactor, respectively, which was 71% and 21% higher than those obtained in the control cultures where a traditional one‐step temperature upshift strategy was applied. In addition, this study transformed empirical fermentation process optimization into a more efficient and rational self‐optimization method. The methodology employed in this study is equally applicable to predict the regulation of process dynamics for other products, thereby facilitating the potential for furthering the intelligent automation of fermentation processes.
Advanced three‐dimensional in vitro liver models to study the activity of anticancer drugs
Authors: Zuchowska Agnieszka , Frojdenfal Sonia , Trzaskowski Maciej , Jastrzebska Elzbieta 
Published: June 2024

AbstractThe liver is one of the most important organs in the human body. It performs many important functions, including being responsible for the metabolism of most drugs, which is often associated with its drug‐induced damage. Currently, there are no ideal pharmacological models that would allow the evaluation of the effect of newly tested drugs on the liver in preclinical studies. Moreover, the influence of hepatic metabolism on the effectiveness of the tested drugs is rarely evaluated. Therefore, in this work we present an advanced model of the liver, which reflects most of the morphologically and metabolically important features of the liver in vivo, namely: three‐dimensionality, cellular composition, presence of extracellular matrix, distribution of individual cell types in the structure of the liver model, high urea and albumin synthesis efficiency, high cytochrome p450 activity. In addition, the work, based on the example of commonly used anticancer drugs, shows how important it is to take into account hepatic metabolism in the effective assessment of their impact on the target organ, in this case cancer. In our research, we have shown that the most similar to liver in vivo are 3D cellular aggregates composed of three important liver cells, namely hepatocytes (HepG2), hepatic stellate cells (HSCs), and hepatic sinusoidal endothelial cells (HSECs). Moreover, we showed that the cells in 3D aggregate structure need time (cell–cell interactions) to improve proper liver characteristic. The triculture model additionally showed the greatest ability to metabolize selected anticancer drugs.
Functionalized graphene oxide as a nanocarrier for delivering oridonin to improve anti‐breast cancer cell activity
Authors: Zhang Xin‐yan , Shi Xiao‐zi , Yu Jia‐yuan , Wang Jin , Zhao Yong‐ming 
Published: June 2024

AbstractIn this study, a targeted nanocarrier was developed by functionalizing graphene oxide with polyethyleneimine and folic acid, intended for loading oridonin. The nanocarrier was successfully synthesized and characterized using an ultraviolet spectrum, Fourier transform infrared spectroscopy and scanning electron microscopy. The nanocarrier demonstrated a remarkable oridonin loading capacity, reaching 424.8 μg/mg, as determined by ultra‐high performance liquid chromatography. In vitro drug release experiments exhibited a pH‐dependent release profile, with a higher cumulative release in an acidic environment. The release mechanism followed the Ritger–Peppas equation model. Cytotoxicity assays indicated minimal toxicity of the nanocarrier. Enhanced cellular uptake by MCF7 cells was observed for carriers functionalized with folate and polyethyleneimine. These findings highlight the potential of functionalized graphene oxide as a promising carrier for oridonin delivery in biomedical applications.
Preparing counselors‐in‐training to address religion and spirituality: Fostering competency through the lens of the addiction curriculum
Authors: Hagedorn W. Bryce , Butler Sabrina , Frank Alexandra 
Published: June 2024

AbstractBeginning with a brief review of the literature related to the importance of religion/spirituality (R/S) in counseling clients with substance use disorders and addictions, this article provides classroom exercises that educators can use in the addiction curriculum to foster student insight and awareness, leading to increased R/S competency.
A pragmatic guide to generic qualitative research in counselor education
Authors: Waalkes Phillip L. , DeCino Daniel A. , Somerville Tiffany , Phelps‐Pineda Monica Maria , Flynn Stephen V. , LeBlanc Joe 
Published: June 2024

AbstractFew practical guidelines exist for conducting generic qualitative research (GQR) with rigor and intentional purpose. This article offers varied purposes of GQR (practicality and creating change, flexible blending of methods, and innovation) and pragmatic guidance for counselor educators and doctoral students conceptualizing, designing, and conducting rigorous GQR studies.
Development of neurofeedback training competencies for counselors: A Delphi study
Authors: Spears James R. , Romero Devon E. , Robertson Derek L. , Prasath Priscilla Rose 
Published: June 2024

AbstractA three‐round Delphi method surveyed 17 experts, identifying 108 essential neurofeedback competencies for counselors‐in‐training. This research addresses concerns about the impact of neuro‐informed practices on counselor identity and scope of practice, providing a foundation for incorporating neurofeedback training into counselor education.
Enhancing Binding Affinity to CGG/CGG Triad: Optimizing Naphthyridine Carbamate Dimer Derivatives with Varied Linker Lengths
Authors: Shibata Tomonori , Nakamachi Ayano , Nakatani Kazuhiko 
Published: June 2024

AbstractThis study examines the binding properties of six naphthyridine carbamate dimer (NCD) derivatives with varying linker lengths to the CGG/CGG triad, a non‐canonical DNA structure linked to repeat expansion disorders. By altering the linker length from 2 to 4 methylene groups, we found changes in thermal stability of the ligand‐bound complexes while maintaining a consistent 2 : 1 binding stoichiometry. Among the derivatives, CC23 showed superior binding affinity compared to the parent molecule CC33 (NCD). Spectroscopic analyses revealed that linker length influences the conformational equilibrium of NCD derivatives. Thermal melting temperature measurements demonstrated CC23′s enhanced thermal stability over CC33. These findings underscore the potential of optimized NCD derivatives, like CC23, as tools to modulate CGG repeat structures, offering insights for therapeutic strategies targeting repeat expansion disorders.
Enhancing UAV‐HetNet security through functional encryption framework
Authors: Gupta Sachin Kumar , Gupta Parul , Singh Pawan 
Published: June 2024

SummaryIn the current landscape, the rapid expansion of the internet has brought about a corresponding surge in the number of data consumers. As user volume and diversity have escalated, the shift from conventional, uniform networks to Heterogeneous Networks (HetNets) has emerged. HetNets are designed with a primary objective: enhancing Quality of Service (QoS) standards for users. In the context of HetNets facilitated by Unmanned Aerial Vehicles (UAVs), a substantial influx of users and devices is observed. Within this multifaceted environment, the potential for malicious intruder nodes to efficiently execute and propagate harmful actions across the network is a distinct concern. Consequently, the entirety of network communication becomes susceptible to a multitude of security threats. To address these vulnerabilities and safeguard communication, the Functional Encryption (FE) technique is employed. FE empowers the protection of data against intrusion attacks. This paper presents a comprehensive methodology for implementing FE within UAV‐integrated HetNets, executed in two sequential phases. The initial phase secures communication between User Equipment (UE) and Micro Base Station (MBS), followed by the second phase, which focuses on securing communication among MBS and UAV. The viability of the proposed approach is substantiated through validation using the Automated Validation of Internet Security Protocols and Applications (AVISPA) tool. The validation process involves the development of High‐Level Protocol Specification Language (HLPSL) codes. The successful security validation outcome underscores the capacity of the proposed methodology to provide the intended security measures and robustness to the network environment.