bluuebunny/crossref_metadata_2025_split huggingface dataset:
Is blood blood? Comparing quantitation of endogenous steroids and luteinizing hormone in concurrently collected venous serum and Tasso+ SST capillary serum samples
Authors: Goodrum Jenna M. , Peek Katy , Moore Chad , Eichner Daniel , Miller Geoffrey D. 
Published: May 2024

AbstractThe monitoring of endogenous steroids in urine has been an important component of the Athlete Biological Passport (ABP) for the last decade. Recently, the quantitation of endogenous steroids in blood has been incorporated into the ABP to increase sensitivity in circumstances where the excretion of urinary ABP biomarkers is low. Current ABP guidelines mandate the use of venous blood draws for blood steroid sample collections, however, recent efforts have focused on investigating the use of less invasive sample collection methods, such as capillary blood collected from the upper arm. The focus of this study was to compare the analytical results of venous and capillary blood collected weekly from 20 individuals, 10 males and 10 females, over six weeks. The two primary biomarkers of the blood steroid ABP module, testosterone (T) and the testosterone/androstenedione (T/A4) ratio, were compared, as well as luteinizing hormone (LH) and the T/LH ratio in male participants, two biomarkers known to be responsive to T use. All biomarkers showed excellent agreement between venous and capillary blood. Longitudinal stability between sample types within individuals was also comparable for all biomarkers. Finally, storage of simultaneously collected capillary samples at room temperature and frozen conditions was compared with evaluate the potential impact of non‐cold chain shipping conditions. Most biomarkers showed excellent agreement between frozen and room temperature storage conditions. These results indicate capillary blood collections represent a promising alternative to venous blood collections for the blood steroid module of the ABP.
Effects of antibiotic resistance genes on health risks of rivers in habitat of wild animals under human disturbance – based on analysis of antibiotic resistance genes and virulence factors in microbes of river sediments
Authors: Hu Rongpan , Ren Minxing , Liang Sumei , Zou Shuzhen , Li Dayong 
Published: May 2024

AbstractStudying the ecological risk of antibiotic resistance genes (ARGs) to wild animals from human disturbance (HD) is an important aspect of “One Health”. The highest risk level of ARGs is reflected in pathogenic antibiotic‐resistant bacteria (PARBs). Metagenomics was used to analyze the characteristics of PARBs in river sediments. Then, the total contribution of ARGs and virulence factors (VFs) were assessed to determine the health risk of PARBs to the rivers. Results showed that HD increased the diversity and total relative abundance of ARG groups, as well as increased the kinds of PARBs, their total relative abundance, and their gene numbers of ARGs and VFs. The total health risks of PARBs in wild habitat group (CK group), agriculture group (WA group), grazing group (WG group), and domestic sewage group (WS group) were 0.067 × 10−3, −1.55 × 10−3, 87.93 × 10−3, and 153.53 × 10−3, respectively. Grazing and domestic sewage increased the health risk of PARBs. However, agriculture did not increase the total health risk of the rivers, but agriculture also introduced new pathogenic mechanisms and increased the range of drug resistance. More serious was the increased transfer risk of ARGs in the PARBs from the rivers to wild animals under agriculture and grazing. If the ARGs in the PARBs are transferred from the rivers under HD to wild animals, then wild animals may face severe challenges of acquiring new pathogenic mechanisms and developing resistance to antibiotics. Further analysis showed that the total phosphorus (TP) and dissolved organic nitrogen (DON) were related to the risk of ARGs. Therefore, controlling human emissions of TP and DON could reduce the health risk of rivers.
Crepuscular rays — The bright side of complement after tissue injury
Authors: Mannes Marco , Savukoski Susa , Ignatius Anita , Halbgebauer Rebecca , Huber‐Lang Markus 
Published: May 2024

AbstractAcute injuries trigger an intense activation of the body's defense mechanisms aiming to limit damage and initiate healing. Among the crucial components of the intravascular immune system, the complement system plays a significant role in traumatic injuries, albeit often negatively. It has been suggested that excessive activation of the complement system, transitioning from a localized and timed response to a systemic one, can lead to a loss of its host‐protective characteristics. Complement activation products have been associated with the severity of injuries, which sometimes serve as predictors for the onset of organ dysfunctions. Animal studies utilizing complement‐targeting agents have provided the basis for considering complement in the management of traumatic injuries in humans. However, numerous studies suggest that the spatial and temporal aspects of complement inhibition are crucial for its efficacy. Understanding the underlying mechanism of the injury is essential to determine where, when, and whether complement inhibition is warranted. Despite the detrimental effects of uncontrolled complement activation, its regulated activation may contribute to essential aspects of healing, such as waste removal and regeneration. This review focuses on the beneficial roles of complement activation in trauma, which are often overlooked or given less consideration but are of immense importance.
Recent Advances in Developing Radical Methods for the Synthesis of Aliphatic Sulfonyl Fluorides
Authors: Lin Lu , Pei Guanhua , Cao Zhong‐Yan , Liao Saihu 
Published: May 2024

AbstractSulfonyl fluorides have widespread applications in many fields, including organic synthesis, chemical biology, drug discovery and materials science. In particular, in the past decade, a number of aliphatic sulfonyl fluorides have been identified showing various biological activities. These applications and appealing features of sulfonyl fluorides have brought about a significant advancement in developing synthetic methods to access sulfonyl fluorides. In this review, we will discuss the recent developments of radical approaches for the synthesis of aliphatic sulfonyl fluorides.
Keeping score: Past victimization reduces offenders’ conciliatory sentiments for their present transgressions
Authors: Thai Michael , Wenzel Michael , Quinney Blake , Woodyatt Lydia , Okimoto Tyler G. 
Published: May 2024

AbstractMany relationships within which interpersonal transgressions transpire often involve histories of reciprocal wrongdoing, where each party has occupied both the role of victim and offender. We investigate whether past incidents of being wronged by the victim of a present transgression may dampen offenders’ conciliatory sentiments for their present wrongdoing. Across four studies (combined N = 1037), we find evidence that past victimization experiences within the context of an interpersonal relationship can blur offenders’ construal of their role as offender and elicit feelings of victimhood, allowing them to exonerate themselves and feel less guilt for their present wrongdoing, display less empathy for the present victim, and reduce their willingness to reconcile with the present victim. These findings highlight the importance of taking into account historical transgressions within a relationship as a determinant of relationship repair in the aftermath of present wrongdoings.
Multiphase electroextraction of malachite green from surface water and its determination using digital imaging and chemometric tools
Authors: Viana Jaime dos Santos , Kubáň Pavel , Botelho Bruno Gonçalves , Orlando Ricardo M. 
Published: May 2024

AbstractThis study introduces a novel method for the quantification of malachite green (MG), a pervasive cationic dye, in surface water by synergizing multiphase electroextraction (MPEE) with digital image analysis (DIA) and partial least square discriminant analysis. Aimed at addressing the limitations of conventional DIA methods in terms of quantitation limits and selectivity, this study achieves a significant breakthrough in the preconcentration of MG using magnesium silicate as a novel sorbent. Demonstrating exceptional processing efficiency, the method allows for the analysis of 10 samples within 20 min, exhibiting remarkable sensitivity and specificity (over 0.95 and 0.90, respectively) across 156 samples in both training and test sets. Notably, the method detects MG at low concentrations (0.2 µg L−1) in complex matrices, highlighting its potential for broader application in environmental monitoring. This approach not only underscores the method's cost‐effectiveness and simplicity but also its precision, making it a valuable tool for the preliminary testing of MG in surface waters. This study underscores the synergy among MPEE, DIA, and chemometric tools, presenting a cost‐efficient and reliable alternative for the sensitive detection of water contaminants.
Experiment study on focusing pattern prediction of particles in asymmetric contraction–expansion array channel
Authors: Shi Xin , He Qing , Tan Wei , Lu Yuwen , Zhu Guorui 
Published: May 2024

AbstractContraction‐expansion array (CEA) microchannel is a typical structure applied on particle/cell manipulation. The prediction of the particle focusing pattern in CEA microchannel is worthwhile to be investigate deeply. Here, we demonstrated a virtual boundary method by flow field analysis and theoretical derivation. The calculating method of the virtual boundary location, related to the Reynolds number (Re) and the structure parameter RW, was proposed. Combining the approximate Poiseuille flow pattern based on the virtual boundary method with the simulation results of Dean flow, the main line pattern and the main/lateral lines pattern were predicted and validated in experiments. The transformation from the main line pattern to the main/lateral lines pattern can be facilitated by increasing Re, decreasing RW, and decreasing α. An empirical formula was derived to characterize the critical condition of the transformation. The virtual boundary method can provide a guidance for asymmetric CEA channel design and contribute to the widespread application of microfluidic particle focusing.
Acoustofluidic manipulation for submicron to nanoparticles
Authors: Wei Wei , Wang Zhaoxun , Wang Bingnan , He Xinyuan , Wang Yaping , Bai Yang , Yang Qingrui , Pang Wei , Duan Xuexin 
Published: May 2024

AbstractParticles, ranging from submicron to nanometer scale, can be broadly categorized into biological and non‐biological types. Submicron‐to‐nanoscale bioparticles include various bacteria, viruses, liposomes, and exosomes. Non‐biological particles cover various inorganic, metallic, and carbon‐based particles. The effective manipulation of these submicron to nanoparticles, including their separation, sorting, enrichment, assembly, trapping, and transport, is a fundamental requirement for different applications. Acoustofluidics, owing to their distinct advantages, have emerged as a potent tool for nanoparticle manipulation over the past decade. Although recent literature reviews have encapsulated the evolution of acoustofluidic technology, there is a paucity of reports specifically addressing the acoustical manipulation of submicron to nanoparticles. This article endeavors to provide a comprehensive study of this topic, delving into the principles, apparatus, and merits of acoustofluidic manipulation of submicron to nanoparticles, and discussing the state‐of‐the‐art developments in this technology. The discourse commences with an introduction to the fundamental theory of acoustofluidic control and the forces involved in nanoparticle manipulation. Subsequently, the working mechanism of acoustofluidic manipulation of submicron to nanoparticles is dissected into two parts, dominated by the acoustic wave field and the acoustic streaming field. A critical analysis of the advantages and limitations of different acoustofluidic platforms in nanoparticles control is presented. The article concludes with a summary of the challenges acoustofluidics face in the realm of nanoparticle manipulation and analysis, and a forecast of future development prospects.
Differences in treatment trajectory following brief paediatric inpatient admissions for children and young people with eating disorders
Authors: El‐Salahi Shama , Anderson Ciorsdan , McDaid Alannah , Lunn Amy , Ralph Emily , Holliday Joanna 
Published: May 2024

AbstractObjectiveLittle is known about factors associated with treatment trajectory following brief paediatric admissions for children and young people (CYP) admitted for medical complications of their eating disorder (ED). This project aimed to identify possible factors and ways to improve the usefulness of paediatric admissions.MethodRetrospective NHS data was analysed to explore differences between paediatric admissions followed by community‐based care or inpatient psychiatric care. Twelve parents were interviewed to seek feedback about paediatric admissions.ResultsPatients who received subsequent inpatient psychiatric care were unwell for longer, had longer paediatric admissions and more crisis team input, were more likely to have had previous admissions, and had higher parent‐reported anxiety and depression. However, the groups did not significantly differ in ED severity. The interviews identified recommendations for improving paediatric admissions, which included improving understanding of EDs, enhancing communication channels, and providing psychological support to parents.ConclusionsFactors linked with illness severity (but not illness severity itself) appear to be associated with the difference between CYP either returning to community‐based care or requiring more intensive psychiatric input. These factors may help clinicians understand who requires subsequent inpatient care, allowing clinicians to target more intensive support earlier and facilitate smoother transitions between services.
Exploring precision treatments in immune‐mediated inflammatory diseases: Harnessing the infinite potential of nucleic acid delivery
Authors: Xu Lingxiao , Shao Zhenxuan , Fang Xia , Xin Zengfeng , Zhao Shenzhi , Zhang Hongbo , Zhang Yu , Zheng Wenbiao , Yu Xiaohua , Zhang Zengjie , Sun Lingling 
Published: May 2024

AbstractImmune‐mediated inflammatory diseases (IMIDs) impose an immeasurable burden on individuals and society. While the conventional use of immunosuppressants and disease‐modifying drugs has provided partial relief and control, their inevitable side effects and limited efficacy cast a shadow over finding a cure. Promising nucleic acid drugs have shown the potential to exert precise effects at the molecular level, with different classes of nucleic acids having regulatory functions through varying mechanisms. For the better delivery of nucleic acids, safe and effective viral vectors and non‐viral delivery systems (including liposomes, polymers, etc.) have been intensively explored. Herein, after describing a range of nucleic acid categories and vectors, we focus on the application of therapeutic nucleic acid delivery in various IMIDs, including rheumatoid arthritis, inflammatory bowel disease, psoriasis, multiple sclerosis, asthma, ankylosing spondylitis, systemic lupus erythematosus, and uveitis. Molecules implicated in inflammation and immune dysregulation are abnormally expressed in a series of IMIDs, and their meticulous modulation through nucleic acid therapy results in varying degrees of remission and improvement of these diseases. By synthesizing findings centered on specific molecular targets, this review delivers a systematic elucidation and perspective towards advancing and utilization of nucleic acid therapeutics for managing IMIDs.
Impact of electrode arrangement and electrical connections on the power generation of ceramic membrane microbial fuel cell
Authors: Rojas Fernando A. , Hernández‐Benitez Carlos , Ramírez Víctor , Ieropoulous Ioannis , Godínez Luis A. , Robles Irma , Meza David B. , Rodríguez‐Valadez Francisco J 
Published: May 2024

AbstractSome of the most popular technologies for wastewater sanitation, still face serious limitations related to high energy consumption requirements. In this context, microbial fuel cells (MFCs) constitute a promising approach since they do not require aeration and produce electricity at the same time. Limitations for these devices, however, are related to the cost of the constituents and the functionality of the arrangement. In this work, a semi‐cylindrical ceramic MFC was designed and constructed using a low‐cost commercial ceramic handcraft as a membrane, carbon felt, carbon cloth, and carbon cloth/activated carbon in different arrangements for the anode and cathode components. The best results were obtained using carbon felt as an anode and a cathodic zone built with carbon felt in which void regions were filled with activated carbon. This arrangement produced 85 mWm−2 for each cell. Evaluating the performance of the MFC in a modular system with eight cells using a different number of separations inside the module and different electrical connections, resulting in a 4‐compartment module that produced 90 mWm−2 with one single module and 95 mWm−2 with a serial arrangement of two modules.
Antagonistic actions of PAK1 and NF2/Merlin drive myelin membrane expansion in oligodendrocytes
Authors: Baudouin Lucas , Adès Noémie , Kanté Kadia , Bachelin Corinne , Hmidan Hatem , Deboux Cyrille , Panic Radmila , Ben Messaoud Rémy , Velut Yoan , Hamada Soumia , Pionneau Cédric , Duarte Kévin , Poëa‐Guyon Sandrine , Barnier Jean‐Vianney , Nait Oumesmar Brahim , Bouslama‐Oueghlani Lamia 
Published: May 2024

AbstractIn the central nervous system, the formation of myelin by oligodendrocytes (OLs) relies on the switch from the polymerization of the actin cytoskeleton to its depolymerization. The molecular mechanisms that trigger this switch have yet to be elucidated. Here, we identified P21‐activated kinase 1 (PAK1) as a major regulator of actin depolymerization in OLs. Our results demonstrate that PAK1 accumulates in OLs in a kinase‐inhibited form, triggering actin disassembly and, consequently, myelin membrane expansion. Remarkably, proteomic analysis of PAK1 binding partners enabled the identification of NF2/Merlin as its endogenous inhibitor. Our findings indicate that Nf2 knockdown in OLs results in PAK1 activation, actin polymerization, and a reduction in OL myelin membrane expansion. This effect is rescued by treatment with a PAK1 inhibitor. We also provide evidence that the specific Pak1 loss‐of‐function in oligodendroglia stimulates the thickening of myelin sheaths in vivo. Overall, our data indicate that the antagonistic actions of PAK1 and NF2/Merlin on the actin cytoskeleton of the OLs are critical for proper myelin formation. These findings have broad mechanistic and therapeutic implications in demyelinating diseases and neurodevelopmental disorders.
Prognostic factors of tongue cancer in children and adolescents: A SEER population‐based study
Authors: Jiang Zhihua , Yao Xianming , Lou Yi , Feng Shaoguang 
Published: May 2024

AbstractPurposeThis study aims to analyze the clinicopathological characteristics and survival outcomes of tongue cancer in the pediatric population, a topic with limited existing data, using a population‐based cohort.MethodsPediatric patients diagnosed with tongue cancer from 1975 to 2018 were identified from the Surveillance, Epidemiology, and End Results (SEER) database. Survival rates were assessed using Kaplan–Meier analysis. Univariate survival analysis was conducted with the log‐rank test, while multivariate analysis involved Cox proportional‐hazards regression to identify factors influencing overall survival (OS). A predictive nomogram was developed based on Cox regression findings.ResultsIn total, 97 pediatric patients with tongue cancer were identified, with a median age at diagnosis of 15 years (range: 1–19 years). Tumors were classified as squamous cell carcinoma (45.4%), rhabdomyosarcoma (RMS) (13.4%), and others (41.2%). Of the patients, squamous cell carcinoma was more common in older children, whereas rhabdomyosarcoma was more common in younger children. The Cox proportional hazard regression revealed that histology and surgery were significant independent predictors of overall survival. The chance of death increased with no surgery. Moreover, patients with squamous cell carcinoma or rhabdomyosarcoma have a poorer survival percentage than patients with other subtypes.ConclusionsTongue cancer in children is rare and associated with poor survival outcomes. This study highlights the significance of tumor histology and surgical intervention in determining overall survival, offering valuable insights for clinical decision‐making in pediatric tongue cancer.
Theoretical study of structure sensitivity on ceria‐supported single platinum atoms and its influence on carbon monoxide adsorption
Authors: Salichon Antoine , Salcedo Agustin , Michel Carine , Loffreda David 
Published: May 2024

AbstractDensity functional theory (DFT) calculations explore the stability of a single platinum atom on various flat, stepped, and defective ceria surfaces, in the context of single‐atom catalysts (SACs) for the water–gas shift (WGS) reaction. The adsorption properties and diffusion kinetics of the metal strongly depend on the support termination with large stability on metastable and stepped CeO2(100) and (210) surfaces where the diffusion of the platinum atom is hindered. At the opposite, the more stable CeO2(111) and (110) terminations weakly bind the platinum atom and can promote the growth of metallic clusters thanks to fast diffusion kinetics. The adsorption of carbon monoxide on the single platinum atom supported on the various ceria terminations is also sensitive to the surface structure. Carbon monoxide weakly binds to the single platinum atom supported on reduced CeO2(111) and (211) terminations. The desorption of the CO2 formed during the WGS reaction is thus facilitated on the latter terminations. A vibrational analysis underlines the significant changes in the calculated scaled anharmonic CO stretching frequency on these catalysts.
Probing the range of applicability of structure‐ and energy‐adjusted QM/MM link bonds III: QM/MM MD simulations of solid‐state systems at the example of layered carbon structures
Authors: Purtscher Felix R. S. , Hofer Thomas S. 
Published: May 2024

AbstractThe previously introduced workflow to achieve an energetically and structurally optimized description of frontier bonds in quantum mechanical/molecular mechanics (QM/MM)‐type applications was extended into the regime of computational material sciences at the example of a layered carbon model systems. Optimized QM/MM link bond parameters at HSEsol/6‐311G(d,p) and self‐consistent density functional tight binding (SCC‐DFTB) were derived for graphitic systems, enabling detailed investigation of specific structure motifs occurring in graphene‐derived structures quantum‐chemical calculations. Exemplary molecular dynamics (MD) simulations in the isochoric‐isothermic (NVT) ensemble were carried out to study the intercalation of lithium and the properties of the Stone–Thrower–Wales defect. The diffusivity of lithium as well as hydrogen and proton adsorption on a defective graphene surface served as additional example. The results of the QM/MM MD simulations provide detailed insight into the applicability of the employed link‐bond strategy when studying intercalation and adsorption properties of graphitic materials.