bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Protein Tyrosine Kinase Tec Regulates a CD44highCD62L− Th17 Subset
Authors: Boucheron Nicole , Sharif Omar , Schebesta Alexandra , Croxford Andrew , Raberger Julia , Schmidt Uwe , Vigl Benjamin , Bauer Jan , Bankoti Rashmi , Lassmann Hans , Epstein Michelle M. , Knapp Sylvia , Waisman Ari , Ellmeier Wilfried 
Published: September 2010

Abstract
The generation of Th17 cells has to be tightly controlled during an immune response. In this study, we report an increase in a CD44highCD62L− Th17 subset in mice deficient for the protein tyrosine kinase Tec. CD44highCD62L− Tec−/− CD4+ T cells produced enhanced IL-17 upon activation, showed increased expression levels of IL-23R and RORγt, and IL-23–mediated expansion of Tec−/− CD4+ T cells led to an increased production of IL-17. Tec−/− mice immunized with heat-killed Streptococcus pneumoniae displayed increased IL-17 expression levels in the lung postinfection with S. pneumoniae, and this correlated with enhanced pneumococcal clearance and reduced lung inflammation compared with Tec+/+ mice. Moreover, naive Tec−/− OT-II CD4+ T cells produced higher levels of IL-17 when cultured with OVA peptide-loaded bone marrow-derived dendritic cells that have been previously activated with heat-killed S. pneumoniae. Taken together, our data indicated a critical role for Tec in T cell-intrinsic signaling pathways that regulate the in vivo generation of CD44highCD62L− effector/memory Th17 populations.
Critical Role of Pcid2 in B Cell Survival through the Regulation of MAD2 Expression
Authors: Nakaya Teruo , Kuwahara Kazuhiko , Ohta Kazutaka , Kitabatake Masahiro , Toda Teppei , Takeda Naoki , Tani Tokio , Kondo Eisaku , Sakaguchi Nobuo 
Published: September 2010

AbstractThe mitotic checkpoint is essential for maintaining genomic stability in differentiating B cells undergoing genetic alterations of the Ig gene. In this study, using real-time RT-PCR and in situ RNA hybridization, we demonstrated that MAD2 mRNA export is selectively regulated by Pcid2/Thp1. Pcid2 small interfering RNA induced a cell-cycle abnormality with increased apoptosis and polyploidy, as previously observed in MAD2-knockdown cells. Pcid2 small interfering RNA reduced MAD2 expression, but not the expression of other cell-cycle checkpoint proteins, such as MAD1 and BUBR1, or the cell-cycle–associated proteins, cyclin A, cyclin B1, and cyclin-dependent kinase 1. In mouse B lineage cells, Pcid2 transcripts appeared in a stage-dependent manner at high levels in bone marrow pre-B and immature B cells, and in spleen transitional 1 and follicular B cells, but at lower levels in pro-B, transitional 2, and marginal zone B cells, suggesting a stage-dependent requirement for MAD2 regulation. Cd19-cre–derived targeting of the Pcid2 gene induced a mature B cell deficiency in mice. These findings indicate that Pcid2 is essential for B cell survival through the regulation of MAD2 expression during B cell differentiation.
Texture, Microstructure and Mechanical Properties of Ultrafine Grained Aluminum Produced by Accumulative Roll Bonding
Authors: Scharnweber Juliane , Skrotzki Werner , Oertel Carl‐Georg , Brokmeier Heinz‐Günter , Höppel Heinz Werner , Topic Irena , Jaschinski Jörn 
Published: September 2010

AbstractTexture, microstructure, and tensile behavior of technically pure aluminum AA1050 and of the age‐hardening alloy AA6016 produced by accumulative roll bonding (ARB) were studied for different numbers of ARB cycles. After eight cycles an ultrafine grained microstructure with grain sizes of the order of 0.5 µm is reached. The grain size decreases with increasing alloying content. The texture consists of a major copper component and a minor brass component. The latter strengthens with alloying. Due to shear deformation in the surface region also a rotated cube component is found. It is stronger in the pure material and is partly added up in the bulk during ARB. Due to dislocation and grain boundary hardening the tensile strength increases with increasing ARB cycles following the Hall‐Petch behavior while a moderate ductility is kept. Within the sheet plane no significant influence of the tensile direction on the observed mechanical properties was found.
The effects of particle size on the mechanisms of atomization of suspensions using hydraulic spray nozzles
Authors: Addo‐Yobo F. O. , Pitt M. J. , Obiri H. A. 
Published: September 2010

AbstractA study of the effects of particles on the manner in which sheets of aqueous suspensions wetted particles disintegrate during atomization by hydraulic pressure nozzle showed that the sheet stability is reduced by particles in the region where the particle size is greater than the sheet thickness due to localized thinning of the sheet due to curvature variations caused by particles moving apart in this region. At high‐volume fraction of solids, the sheets perforate. It is inferred from the mechanism that the critical volume fraction of solids at the onset of perforation decreases with decreasing particle size, decreasing density and surface tension, and increasing viscosity of the medium. A critical perforation number, Ac, is inferred from the above mechanism. Waves are observed on the sheet at positions closer to the nozzle which would be absent on aqueous sheets similarly produced but free of particles. © 2010 American Institute of Chemical Engineers AIChE J, 2011
Kinetics of carbon dioxide chemical absorption into cyclic amines solutions
Authors: García‐Abuín Alicia , Gómez‐Díaz Diego , Navaza José M. , Vidal‐Tato Isabel 
Published: September 2010

AbstractChemical reaction kinetics between carbon dioxide with two cyclic amines (pyrrolidine and piperidine) have been studied using a stirred tank reactor with a planar interfacial area. The operational variables considered in this work have been the amine concentration in the liquid phase and the reaction temperature. Specific absorption rates have been determined under different experimental conditions. Results indicate that the absorption process occurs in a pseudo first reaction regime exhibited first‐order kinetic with respect carbon dioxide and a second order for both cyclic amines. The reaction‐rate constant was determined under the different experimental conditions, and it was correlated depending on the temperature by means of an Arrhenius type equation. © 2010 American Institute of Chemical Engineers AIChE J, 2011
Nest site ecology of the cross river gorilla at the Kagwene Gorilla Sanctuary, Cameroon, with special reference to anthropogenic influence
Authors: De Vere Ruth A. , Warren Ymke , Nicholas Aaron , Mackenzie Mary E. , Higham James P. 
Published: September 2010

AbstractThe Cross River gorilla is the most endangered of all Great Apes. It is currently found in 14 apparently geographically separate and increasingly isolated populations, which face severe threats from habitat loss and the bushmeat trade. Recent years have seen greater efforts to conserve the Cross River gorilla across the landscape, but details of the ecology of individual populations that might help in conservation initiatives are lacking. Here, we report on the production of the first habitat map for the recently created Kagwene Gorilla Sanctuary (KGS), Cameroon, and the results of an analysis of nest site preferences of the gorillas that live there. Qualitative vegetation assessments were made to assign various categories to the topstory and understory throughout the sanctuary, and nest sites constructed between January 2006 and March 2008 were re‐visited and assessed for possible site preferences. The habitat map revealed significant anthropogenic impact, with only 57% of the KGS being relatively undisturbed primary forest. Analysis of nest sites showed that ground nests are constructed preferentially in the dry season, on precipitous slopes, in light gaps and clearings, with an understory of mixed herbs. Tree nests are predominantly built in the wet season, in primary forest with saplings as the preferred understory. Gorillas avoid nesting in grasslands and farms, which visibly fragment the remaining forest in the sanctuary. The results have implications for the conservation and management of the Cross River gorilla at KGS, and offer new insight into the nesting ecology of this subspecies. Am. J. Primatol. 73:253–261, 2011. © 2010 Wiley‐Liss, Inc.
Characterization of terpolymers containing 1,2,4‐oxadiazolic pendant groups with potential application as workover fluids
Authors: Vega Isabel , Lamanna Melisa , Fissore Eliana , D'Accorso Norma , Rojas Ana M. 
Published: September 2010

AbstractPresence of planar, bulky heterocyclic pendant groups, attached to polymer chains, alters the thermal properties and, once gelled, they modify their rheological properties as well. The authors report generation of stiff gels coming from terpolymers containing 1,2,4‐oxadiazolic pendant groups, obtained by chemical modification of commercial polyacrylonitrile. The gels formed in basic aqueous solutions were compared and the effect of substituents linked to the heterocycles on thermal stability and viscoelastic properties was also analyzed. The structural modifications were followed by FTIR. The potential use of these terpolymers as workover fluids is discussed. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Synthesis and characterization of cation‐exchange membrane based on sulfonated sty/HEA/LMA terpolymer
Authors: Shin Kyoung Sub , Choi Eun Mi , Hwang Taek Sung 
Published: September 2010

AbstractA cation‐exchange membrane based on a styrene/hydroxyethyl acrylate/lauryl methacrylate (Sty/HEA/LMA) terpolymer was prepared via a postsulfonation reaction for various sulfonation times. Sulfonic groups were introduced into the membrane structure with sulfuric acid as the sulfonating agent and silver sulfate as an initiator in a nitrogen atmosphere. Sulfonated Sty/HEA/LMA terpolymer membranes were characterized by Fourier transform infrared (FTIR) spectrometry and nuclear magnetic resonance as well as by determining the degree of sulfonation (DS), ion‐exchange capacity (IEC), water uptake (WU), and electrical property of the membranes. The presence of sulfonic groups in the sulfonated Sty/HEA/LMA terpolymer was confirmed by FTIR, and the resulting membrane showed an IEC of 1.29 meq/g and an electrical resistance of 0.1 Ω cm2. The WU of the prepared membranes increased with the DS at the reaction time. The surface morphology obtained by atomic force microscopy clearly showed an increase of roughness with reaction time. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Synthesis and characterization of poly{N‐[3‐(dimethylamino) propyl] methacrylamide‐co‐itaconic acid} hydrogels for drug delivery
Authors: Mishra Rakesh K. , Ray Alok R. 
Published: September 2010

AbstractA novel pH‐sensitive hydrogel system composed of itaconic acid (IA) and N‐[3‐(dimethylamino) propyl] methacrylamide was designed. This system was prepared by aqueous copolymerization with N,N‐methylene bisacrylamide as a chemical crosslinker. The chemical structure of the hydrogels was characterized by Fourier transform infrared (FTIR) spectroscopy. The microstructure and morphology of the hydrogels were evaluated by X‐ray diffraction (XRD) and scanning electron microscopy (SEM). The SEM study of hydrogels on higher magnification revealed a highly porous morphology with uniformly arranged pores ranging from 40 to 200 μm in size. XRD analysis revealed the amorphous nature of the hydrogels, and it was found that an increase in the IA content in the monomer feed greatly reduced the crystallinity of the hydrogels. Swelling experiments were carried out in buffer solutions at different pH values (1.2–10) at 37°C ± 1°C to investigate their pH‐dependent swelling behavior and dimensional stability. An increase in the acid part (IA) increased the swelling ratio of the hydrogels. Temperature‐sensitive swelling of the hydrogels was investigated at 20–70°C in simulated intestinal fluid. The hydrogels swelled at higher temperatures and shrank at lower temperatures. 5‐Aminosalicylic acid (5‐ASA) was selected as a model drug, and release experiments were carried out under simulated intestinal and gastric conditions. 5‐ASA release from the poly N‐[3‐(dimethylamino) propyl] methacrylamide‐co‐itaconic acid‐80 (PDMAPMAIA‐80) hydrogel was found to follow non‐Fickian diffusion mechanism under gastric conditions, and a super case II transport mechanism was found under intestinal conditions. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2011
Undergraduate student perceptions of the use of ultrasonography in the study of “Living Anatomy”
Authors: Ivanusic Jason , Cowie Brian , Barrington Michael 
Published: September 2010

AbstractUltrasonography is a noninvasive imaging modality, and modern ultrasound machines are portable, inexpensive (relative to other imaging modalities), and user friendly. The aim of this study was to explore student perceptions of the use of ultrasound to teach “living anatomy”. A module utilizing transthoracic echocardiography was developed and presented to undergraduate medical, science, and dental students at a time they were learning cardiac anatomy as part of their curriculum. Relevant cardiac anatomy was explored on a student volunteer and images were projected in real‐time to all students via an AV projection system. Students were asked to complete a questionnaire about the learning experience and were given the opportunity to provide open feedback. The students' evaluations of this learning experience were very positive. They agreed or strongly agreed that it was an effective way to teach anatomy (90% medical; 77% dental; 100% science) and that it was incorporated in a way that promoted reinforcement of the lecture material (83% medical; 76% dental; 100% science). They agreed or strongly agreed with statements that the experience was innovative (93% medical; 92% dental; 100% science) and stimulated interest in the subject matter (86% medical; 75% dental; 96% science), and that they would like to see more modules, exploring other anatomical sites, incorporated into the curricula (83% medical; 72% dental; 100% science). We believe that ultrasound could be a useful tool, in conjunction with traditional teaching methods, to reinforce the learning of anatomy of a variety of different undergraduate student groups. Anat Sci Educ. © 2010 American Association of Anatomists.
Analysis of peak and non‐peak traffic forecasts using combined models
Authors: Zhang Yang , Liu Yuncai 
Published: September 2010

AbstractAccurate and timely traffic forecasting is crucial to effective management of intelligent transportation systems (ITS). To predict travel time index (TTI) data, we select six baseline individual predictors as basic combination components. Applying the one‐step‐ahead out‐of‐sample forecasts, the paper proposes several linear combined forecasting techniques. States of traffic situations are classified into peak and non‐peak periods. Based on detailed data analyses, some practical guidance and comments are given in what situation a combined model is better than an individual model or other types of combined models. Indicating which model is more appropriate in each state, persuasive comparisons demonstrate that the combined procedures can significantly reduce forecast error rates. It reveals that the approaches are practically promising in the field. To the best of our knowledge, it is the first time to systematically investigate these approaches in peak and non‐peak traffic forecasts. The studies can provide a reference for optimal forecasting model selection in each period. Copyright © 2010 John Wiley & Sons, Ltd.
Operational impacts of incident quick clearance legislation: a simulation analysis
Authors: Fries Ryan , Hamlin Carol , Chowdhury Mashrur , Ma Yongchang , Ozbay Kaan 
Published: September 2010

AbstractMany states in the US have enacted quick clearance laws requiring drivers of vehicles involved in minor incidents to move their vehicles from travel lanes prior to the arrival of first responders. Since little is known about the effectiveness of these laws, this research sought to find the benefit–cost ratio of advertising quick clearance legislation to improve driver compliance, and compare it with benefit–cost ratios of other incident management strategies, particularly traffic cameras, freeway service patrols, and traffic sensors. The analysis used traffic simulation that applied application programming interfaces to produce random spatial and temporal occurrence of incidents, including incident start times, durations, and locations, based on normal distributions developed from field data, to test before and after the law scenarios. The results provide decision makers with support for prioritizing funding between these incident management strategies and indicated that investments in the advertisement of this law was beneficial. Copyright © 2011 John Wiley & Sons, Ltd.
Understanding members' carsharing (activity) persistency by using econometric model
Authors: Morency Catherine , Habib Khandker M. Nurul , Grasset Vincent , Islam Md Tazul 
Published: September 2010

AbstractCarsharing is an innovative travel alternative that has recently experienced considerable growth and become part of sustainable transportation initiatives. Although carsharing is becoming increasingly a popular alternative transportation mode in North America, it is still an under‐researched area. Current research is aimed at better understanding of the behavior of carsharing users. For every member, a two‐stage approach microsimulates the probability of being active in any month using a binary probit model and given that a particular member is active during a month, the probability of that member using the service multiple times using a random utility‐based model. The model is estimated using empirical data from one of the largest carsharing companies in North America. The model estimates reveal that the activity persistency of members is positively linked to previous behaviors for up to 4 months, and that the influence of previous months weakens over time. It also shows that some attributes of the traveler (gender, age, and language spoken at home) impact his or her behaviors. Copyright © 2010 John Wiley & Sons, Ltd.
Bezafibrate induced increase in mitochondrial electron transport chain complex IV activity in human astrocytoma cells: Implications for mitochondrial cytopathies and neurodegenerative diseases
Authors: Ioannou Nicola , Hargreaves Iain P. , Allen George , Duberley Kate , Land John M. , Heales Simon J.R. 
Published: September 2010

AbstractMitochondrial encephalomyopathies resulting from electron transport chain (ETC) dysfunction can present with a wide spectrum of clinical manifestations having significant neuropathology and a progressive nature. Despite advances in diagnosis of ETC disorders, treatment still remains inadequate. A recent study in fibroblasts and myoblasts revealed the ability of fibrate treatment to correct ETC enzyme deficiencies. Therefore, fibrates may represent potential therapeutic agents to correct the neurological ETC impairment responsible for the encephalopathic presentation of these disorders. Consequently, this study assessed the effect of bezafibrate on human astrocytoma (HA) 1321N cell ETC activity and coenzyme Q10 (CoQ10) status. HA cells were incubated for 72 H with 300 μM or 500 μM bezafibrate and for 7 days with only 500 μM bezafibrate. A significant effect on ETC activity was observed after 7 days incubation with 500 μM bezafibrate yielding a 130% (P < 0.05) increase in complex IV activity, accompanied by a 33% (P < 0.05) increase in cellular ATP level and a 25% (P < 0.001) decrease in extracellular lactate/pyruvate ratio compared to control levels. Following 7 days culture with bezafibrate, the CoQ10 status of the HA cells appeared to increase although this was not found to be significant. The results of this study have indicated evidence of a bezafibrate induced increase in ETC complex IV activity. Further studies are required to assess the ability of bezafibrate treatment to correct neurological ETC impairment in available animal models of ETC dysfunction before the therapeutic efficacy of this pharmacological agent can be further considered in the treatment of the encephalopathic presentation of ETC disorders.
Ginger ingredients inhibit the development of diethylnitrosoamine induced premalignant phenotype in rat chemical hepatocarcinogenesis model
Authors: Mansour Mahmoud A. , Bekheet Saleh A. , Al‐Rejaie Salim S. , Al‐Shabanah Othman A. , Al‐Howiriny Tawfeq A. , Al‐Rikabi Ammar C. , Abdo Ayman A. 
Published: September 2010

AbstractTo investigate the possible antitumor activity of ginger extract against hepatic carcinogenesis initiated by diethylnitrosoamines (DEN) and promoted by carbon tetrachloride (CCl4). A total of 60 male Wistar albino rats were divided into four groups with 15 animals in each group. Rats in group 1 (control group) received a single intraperitoneal (i.p.) injection of normal saline. Animals in group 2 were given ginger (50 mg/kg/day) in drinking water for 8 weeks. Rats in group 3 (DEN group) were injected with a single dose of DEN (200 mg/kg, i.p.), 2 weeks later received a single dose of CCl4 (2 mL/kg i.g) by gavage as 1:1 dilution in corn oil. Animals in group 4 (DEN‐ginger group) received the same carcinogenesis induction protocol as in group 3 plus ginger (50 mg/kg/day) in drinking water for 2 weeks before induction of hepatocarcinogenesis and continued throughout the experimental period. DEN‐initiated and CCl4‐promoted hepatocarcinogenesis in male Wistar rats was manifested biochemically by elevation of serum hepatic tumor markers tested; α‐fetoprotein and carcinoembryonic antigen. In addition, hepatocarcinogenesis was further confirmed by a significant increase in hepatic tissue growth factors; vascular endothelial growth factor, basic fibroblast growth factor, and hydroxyproline content. A marked decrease in endostatin and metallothonein were also observed. Long‐term ginger extract administration 2 weeks before induction of hepatocarcinogenesis and throughout the experimental period prevented the decrease of the hepatic content of metallothionein and endostatin and the increase in the growth factors induced by the carcinogen. Moreover, ginger extract normalize serum hepatic tumor markers. Histopathological examination of liver tissue also correlated with the biochemical observations. These findings suggest a protective effect of ginger extract against premalignant stages of liver cancer in the DEN‐initiated and CCl4‐promoted hepatocarcinogenesis model in rats.
Blood glucose response to rate of consumption of digestible carbohydrate
Authors: Whelan William J. , Hollar Danielle , Agatston Arthur , Dodson Hannah J. , Tahal Dimitri S. 
Published: September 2010

AbstractWhen the glycemic response to consuming digestible carbohydrate is measured, little or no attention appears to have been paid to the possible effect on this response of the rate at which the food is consumed. We compared glycemic responses when volunteers ate or drank foods containing digestible carbohydrate as rapidly as possible, or in five equal portions over 12 min. Expecting that the response would be greater when the food was consumed rapidly, we found that the responses were equally and randomly distributed between the two rates of eating. At the same time, marked differences were noted in the responses elicited when different individuals consumed the same foods, leading to an investigation of this phenomenon, published elsewhere.