bluuebunny/crossref_metadata_2025_split huggingface dataset:
Promoting active behaviours in schoolchildren using the socio- ecological model in the physical education class: study protocol of ACTIBESE Project
Authors: Rodríguez-Rodríguez Fernando , Cristi-Montero Carlos , Zurita-Corvalán Natalia , Brand Caroline , Mota Jorge , Chillón Palma , Gaya Anelise Reis , Brazo-Sayavera Javier , Aguilar-Farias Nicolás 
Published: April 2024

Abstract

Background.
The correlation between parental and child physical activity levels has been the subject of significant investigation. Existing research has illuminated this association, particularly emphasising its influence during childhood. However, as children transition into adolescence, this relationship appears to weaken. Despite the recognised importance of parental involvement in promoting physical activity among children, scant attention has been directed towards understanding effective strategies and interventions to bolster this relationship. Consequently, there exists a critical gap in the literature concerning actionable measures to enhance parent-child physical activity engagement during the pivotal stage of adolescence.
Methods. The ACTIBESE project aims to assess and intervene in Chilean schoolchildren’s active behaviours by examining the influence of personal, interpersonal, and school factors. This study includes a cross-sectional and intervention study involving schools in the Valparaíso and Araucanía Regions, representing urban and rural areas. Participants, divided into children (8–11 years old) and adolescents (12–16 years old), will undergo a comprehensive evaluation, including sociodemographic characteristics, physical activity (PA) levels, parental and peer support, teaching styles of the teachers, and school environment characteristics. In the intervention study, a 5-month continuous training program for teachers will be implemented, focusing on improving teaching styles and enhancing teaching competencies related to physical education and health classes. The research will use mixed models, ANCOVA, and logistic regressions for data analysis, network analysis, exploratory factors analysis, and structural equation modelling.
Discussion. Through innovative research methods, interventions involving schoolteachers, and a diverse geographical scope, this project aims to make significant contributions to both the scientific understanding of promoting PA and public policy efforts aimed at improving the health and well-being of school-aged children. The ACTIBESE project's multifaceted approach, considering school, family, and peer influences, is well positioned to contribute significantly to promoting AB and addressing the alarming decline in PA levels among Chilean youth.
Electrochemically synthesized Tin micro-nanometer powders for visible light photocatalytic degradation of Rhodamine B dye from polluted water
Authors: Lu Yukun , Zhang Yaojie , Zhang Jiale , Li Zhaoyang , Hu Feiyang , Pan Duo , Melhi Saad , Shi Xuetao , Amin Mohammed A. , El-Bahy Zeinhom M. 
Published: April 2024

Abstract

Tin (Sn) micro-nanoparticles with special pine tree dendritic morphology were synthesized by using tin foil as the anode and titanium as the cathode through simple anodization method. Surprisingly, it is found that the morphology of Sn particles is closely related to factors such as the type of electrolyte, the concentration of the electrolyte, and the different applied voltages, and briefly discussed the influence of various factors on the growth of Sn particles. In addition, Sn particles are calcined under different temperature conditions to obtain Sn/SnO2 hybrid materials with different tin dioxide (SnO2) contents. The changes in morphology and the phase of SnO2 crystal lattices were characterized by scanning electron microscopy (SEM) and X-ray diffraction (XRD), respectively, which proved the successful synthesis of Sn/SnO2 mixed materials. Finally, the Sn/SnO2 hybrid material with metal-doped modified semiconductor properties was used to photocatalytic degradation of simulated organic pollutants rhodamine B (RhB). It was found that the photocatalytic degradation efficiency of the Sn/SnO2 hybrid material under simulated sunlight conditions is near 90% in 5 h. Therefore, this work provides a convenient and effective environmental protection approach for the treatment of architecture and industrial dyes.
Novel Pathogenic Variant in a Mild Case of Type B Molybdenum Cofactor Deficiency: Case Report and Literature Review
Authors: Kinsinger Morgan , Mithal Divakar S. 
Published: April 2024

Abstract

Background:
Molybdenum cofactor deficiency (MoCD) is a rare metabolic disorder caused by pathogenic variants in the highly conserved biosynthetic pathway of molybdenum cofactor (MoCo), resulting in sulfite intoxication. MoCD may present in a clinically severe, rapidly fatal form marked by intractable seizures after birth, hyperekplexia, microcephaly and cerebral atrophy, or a later onset form with a more varied clinic course. Three types of MoCD have been described based on the effected gene along the MoCo synthesis pathway: type A (MOCS1); type B (MOCS2 or MOCS3) and type C (GPHN). The MOCS2 gene is bicistronic, encoding the small (MOCS2A) and large (MOCS2B) subunits with an overlapping coding region. This case report describes a patient with the first known variant causative of mild disease in the overlapping bicistronic region (c.263 G>C) and the first ever described in the highly conserved C-terminal glycine-glycine motif of MOCS2A.
Case Presentation:
The patient developed normally until age 12 months when she presented in the setting of acute illness withdevelopmental regression, low serum uric acid, and MRI with bilateral globus pallidus (GP) injury. Whole exome sequencing (WES) identified a homozygous variant of unknown significance in the MOCS2 gene and the diagnosis of MoCD type B was confirmed by the patient’s elevated urine sulfocysteine and low uric acid. Nearly two years after her initial presentation she has demonstrated progress in language and motor domains, consistent with a mild phenotype of MoCD.
Conclusions:
The case emphasizes challenges in identifying atypical forms of rare diseases, the importance of whole exome sequencing (WES) to identify mild cases of MoCD, and the ongoing challenges with understanding the MOCS2 gene. While one FDA approved treatment exists for MoCD type A, further research into the mechanisms of phenotype-genotype differences among this patient population may aid in additional therapeutic options for MoCD.
Prognostic of postoperative systemic inflammatory response for gastric cancer
Authors: Fukushima Naoko , Masuda Takahiro , Tsuboi Kazuto , Yuda Masami , Takahashi Keita , Yano Fumiaki , Eto Ken 
Published: April 2024

Abstract

Purpose
The systemic inflammatory response is a significant prognostic indicator in patients with cancer. Few studies have reported a relationship between postoperative systemic inflammatory responses and prognosis in patients with gastric cancer. This study aimed to clarify the efficacy of postoperative systemic inflammatory response in patients with gastric cancer after gastrectomy and to determine which prognostic scoring system is better.
Methods
Overall, 210 patients with gastric cancer who underwent gastrectomy between February 2013 and July 2022 were included. Systemic inflammatory response markers, including the Glasgow prognostic score, prognostic nutritional index, neutrophil-lymphocyte ratio, and platelet-lymphocyte ratio, were calculated before and 1 month after surgery. The relationship between preoperative and postoperative systemic inflammatory responses and the prognosis of patients with gastric cancer was investigated.
Results
In multivariate analysis, postoperative Glasgow prognostic score of 1or 2 (P = 0.04, P < 0.01, respectively), stage II or III (P < 0.01, P < 0.01, respectively), and R1 or R2 curability (P = 0.03, P < 0.01, respectively) were independent and significant predictors of disease-free survival and overall survival. Eighty-seven patients (41%) were classified as having postoperative Glasgow prognostic scores of 1 or 2. Patients with a postoperative Glasgow prognostic score of 1 or 2 had significantly more advanced tumors, more invasive surgery, and more postoperative complications than those with a postoperative Glasgow prognostic score of 0.
Conclusion
The postoperative Glasgow prognostic score was independently associated with poor prognosis and recurrence in patients who underwent gastrectomy for gastric cancer comparing other pre- and post- operative prognostic scoring markers.
Importance of hospital and clinical factors for early mortality in Takotsubo syndrome: Insights from the Swedish Coronary Angiography and Angioplasty Registry
Authors: Gudmundsson Thorsteinn , Redfors Björn , Råmunddal Truls , Angerås Oskar , Petursson Petur , Rawshani Araz , Hagström Henrik , Alfredsson Joakim , Ekenbäck Christina , Henareh Loghman , Skoglund Kristofer , Ljungman Charlotta , Mohammad Moman , Jernberg Thomas , Fröbert Ole , Erlinge David , Omerovic Elmir 
Published: April 2024

Abstract

Background
Takotsubo syndrome (TTS) is an acute heart failure syndrome with symptoms similar to acute myocardial infarction. TTS is often triggered by acute emotional or physical stress and is a significant cause of morbidity and mortality among patients with acute coronary syndromes. Predictors of mortality in patients with TS are not well understood, and there is a need to identify high-risk patients and tailor treatment accordingly. This study aimed to assess the importance of various clinical factors in predicting 30-day mortality in TTS patients using a machine learning algorithm.
Methods
We analyzed data from the nationwide Swedish Coronary Angiography and Angioplasty Registry (SCAAR) for all patients with TTS in Sweden between 2015–2022. Gradient boosting was used to assess the relative importance of variables in predicting 30-day mortality in TTS patients.
Results
Of 3,180 patients hospitalized with TTS, 76.0% were women. The median age was 71.0 years (interquartile range 62–77). The crude all-cause mortality rate was 3.2% at 30 days. Machine learning algorithms by gradient boosting identified treating hospital as the most important predictor of 30-day mortality. This factor was followed in significance by the clinical indication for angiography, creatinine level, Killip class, and age. Other less important factors included weight, height, and certain medical conditions such as hyperlipidemia and smoking status.
Conclusions
In an analysis of all Swedish patients diagnosed with TTS over seven years, using machine learning with gradient boosting, we found that the treating hospital was the most significant predictor of 30-day mortality.
Plasma exosomal miR-125b-5p and miR-143-3p have potential to diagnose breast cancer
Authors: Fan Lei , Li Yao , Huang Jicheng , Hua Bin 
Published: April 2024

Abstract

Background
Breast cancer (BC) is the most common malignancy in women, posing a serious health risk. Exosomal microRNA (miRNA) has emerged as a promising cancer biomarker. Despite its potential, research on exosomal miRNA in BC remains limited. This study aims to identify plasma exosomal miRNAs for diagnosing BC.
Methods
Firstly, plasma exosomal miRNA sequencing was conducted on 45 BC patients and 5 healthy controls, followed by Differential Expression Analysis to screen for differentially expressed miRNAs. The diagnostic value of these miRNAs was assessed using receiver operating characteristic (ROC) curves. Subsequent analysis of candidate miRNAs was carried out in the TCGA database. Target genes of differentially expressed miRNAs were predicted using online databases, and Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses were performed. Finally, a ceRNA network was constructed using Cytoscape.
Results
Our analysis indicates that, compared to normal controls, plasma exosomal miR-125b-5p and miR-143-3p are significantly downregulated in BC patients. The area under the curve (AUC) for exosomal miR-125b-5p is 0.951 (sensitivity 91.1%, specificity 100%), and the AUC for exosomal miR-143-3p is 0.924 (sensitivity 86.7%, specificity 100%). In the TCGA database, the expression of miR-125b-5p and miR-143-3p is downregulated in BC tissues compared to adjacent normal tissues. Target gene prediction and enrichment analyses reveal pathways closely associated with BC occurrence.
Conclusions
Our results demonstrate that plasma exosomal miR-125b-5p and miR-143-3p can distinguish between BC patients and normal controls. The downregulation of plasma exosomal miR-125b-5p and miR-143-3p may potentially serve as a marker for the occurrence of BC.
Mental health nursing consultations in Brazilian primary care: analysis of proposed competencies for advanced practice nurses
Authors: de Almeida Patricia Aline , de Almeida Letícia Yamawaka , Vesga-Varela Andrea Liliana , Barreto Carla Pereira , Carrer Marília Orlandelli , Reis Keila Gisele Lima , Serranegra Nayara Vilela Farias , Neto Manoel Vieira de Miranda , Martiniano Claudia Santos , Bonfim Daiana 
Published: April 2024

Abstract

Background: Primary Health Care (PHC) is described as a key strategy to identifying, managing and coordinating mental health cases. Considering that nurses stand out as an essential element to integrate mental health care in PHC, initiatives to broaden the discussion and incorporate the role of Advanced Practice Nurses (ANP) in this setting, can contribute to reduce disparities in mental health care. Thus, this study aimed to analyze mental health nursing consultations in PHC and investigate whether nurses have care management skills proposed for ANP.
Methods: multicenter study, with a quantitative and qualitative approach, carried out in 17 Primary Care Health Units, distributed in three regions of Brazil, from May to July 2022. Data collection was carried out in two moments of the nurse's professional practice: nursing consultation (recorded using film, with direct and non-participatory observation) and nursing record. From a quantitative perspective and during the first research stage, consultations with ≥50% compliance with the nursing process were selected so that, in the second qualitative stage, through content analysis, the competencies proposed for ANP in PHC were identified.
Results: a total of 49 mental health nursing consultations were performed by 21 nurses. Of these, seven were selected with a score greater than 50% of compliance with the Nursing Process, carried out by three nurses. The consultations presented few competencies in the care management dimension proposed for the ANP, nevertheless, NC presented 39.68% in the care focus, 38.78% in assessment and diagnosis and 47.62% in the provision of care.
Conclusions: nurses who carry out mental health nursing consultations in PHC present, in a scarce and partial way, the competencies proposed for the ANP regarding the domain of care management.
Design and synthesis of novel EGFR kinase inhibitors for the treatment of EGFR C797S mutation in non-small cell lung cancer
Authors: Nie Wenyan , Wang Mengxuan , Xia Zhenhong , Wang Chunlong , Nie Haoran , Zhang Shuai , Qiu Jiaqi , yang Yang , Yao Cuifang , Li Xingshu , An Baijiao 
Published: April 2024

Abstract

Third-generation cutaneous growth factor receptor tyrosine kinase inhibitors have led to impressive advances in the treatment of non-small cell lung cancer. However, acquired resistance significantly limits the clinical application of these agents, with the epidermal growth factor receptor (EGFR)C797S mutation being the primary resistance mechanism. In this study, a series of novel EGFR kinase inhibitors was designed and synthesized for the treatment of the EGFRC797S mutation in non-small cell lung cancer. Structure activity relationship screening revealed that compound 5d was the most effective inhibitor of the C797S mutation. It strongly inhibited Baf3-EGFRL858R/T790M/C797S and Baf3-EGFR 19del/T790M/C797S cell lines, with IC50 values of 0.01836±0.0065 and 0.025±0.005 μM, respectively. Molecular docking studies showed that 5d binds to the EGFRL858R/T790M/C797S and EGFR19del/T790M/C797S proteins effectively. A mechanistic study showed that 5d induced cell cycle arrest in the G2/M phase. Compound 5d caused cell apoptosis in a dose-dependent manner, accompanied by a significant increase in the level of the apoptotic protein cleaved caspase-3. An in vivo xenograft assay of mice with Baf3-EGFR19del/T790M/C797S cells showed that 5d effectively inhibited tumor growth, with inhibition rates of 45.2% (70 mg/kg) and 55.7% (100 mg/kg) being observed. Therefore, 5d is worthy of further investigation as a fourth-generation EGFR inhibitor.
PCSK9 promotes vascular neointimal hyperplasia through non-lipid regulation of vascular smooth muscle cell proliferation, migration, and autophagy
Authors: Zhang Qian , Miao Mengdan , Cao Shanhu , Chen Lixia , Liu Da , Wei Mei , Sun Shaoguang , Jin Sheng , Dong Lihua , Yin Yajuan , Liu Gang , Zheng Mingqi 
Published: April 2024

Abstract

We aim to explore the impact of Proprotein convertase subtilisin-kexin type 9 (PCSK9) and its inhibitor evolocumab on neointimal hyperplasia. Wild type and PCSK9 knockout (PCSK9-/-) mice were subjected to ligation of the common carotid artery, with or without subcutaneous injection of PCSK9i Evolocumab. Mouse aortic vascular smooth muscle (MOVAS) cells were pretreated with evolocumab or under siRNA-mediated suppression of PCSK9, and then exposed to platelet-derived growth factor type BB(PDGF-BB), a major promoter of MOVAS transformation to a proliferative phenotype. PCSK9 was upregulated in ligated carotid arteries and PDGF-BB-treated MOVAS cells. PCSK9-/- mice showed decreased intimal area and intimal/ media area ratio, downregulation of proliferation and autophagy, which was coincidence with wild-type mice treated with evolocumab. In MOVAS cells fortified with evolocumab or silencing of PCSK9, PCNA, Beclin1, p62, LC3 were downregulated, additionally, EdU-positive cells decreased, cell viability reduced, migration ability was weakened, and the number of autophagosomes and autolysosomes decreased after the treatment. We also identified the PI3K/AKT/mTOR signaling molecules as potential PCSK9 targets mediating proliferative effect in MOVAS cells. To sum up, our results suggest that PCSK9 has intrinsic properties to promote proliferation, migration and autophagy in VSMCs independent of its lipid- regulating role. The proliferative effects of PCSK9 may be mediated by the PI3K/AKT/mTOR signaling pathway. These data provide additional evidence for PCSK9i in cardiovascular disease beyond the low-density lipoprotein (LDL)-lowering benefit.
Characterization of Carbapenem-Resistant Escherichia coli From a Tertiary Hospital in Hangzhou, China
Authors: Zeng Lingyi , Hu Kewang , Zheng Zhibei , Yu Hua , Zheng Wei , Xu Qihao , Yan Jiaqi , Xiang Mengdie , Wu Jun , Li Jun 
Published: April 2024

Abstract

Background: In recent years, with the large-scale use of antibiotics, the detection rate and mortality of carbapenem-resistant Escherichia coli (CR-EC) have gradually increased. This study explores the molecular characteristics and prevalence of CR-EC isolated from a tertiary hospital in Hangzhou.
Methods: A total of 10 non-duplicated CR-EC were collected. The minimal inhibitory concentration was determined by microbroth dilution method. Various drug-resistant genes were detected by polymerase chain reaction. The transferability of plasmid was verified by conjugation test and genetic homology was detected by pulsed-field gel electrophoresis. The whole genome is sequenced using the Illumina MiSeq technology.
Results: The strains were of diverse origin and all showed multidrug resistance. blaNDM was the only detected carbapenem gene with a high carrying rate (80%). The success rate of conjugation was 75%, indicating the horizontal transfer of blaNDM. Homology analysis showed that there was no widespread cloning outbreak of CR-EC in this study, blaNDM-5-ST410 is prevalent in the local area as a dominant group. WGS suggests complex plasmid replicon situations and greater potential risks of drug resistance and virulence.
Conclusions: This is a survey on the prevalence and molecular characteristics of CR-EC in the Hangzhou, blaNDM-like production combined with ESBLs and/or AmpC was the main resistance mechanism of CR-EC. Complex drug resistance, potential virulence risks and the transferability of plasmids have intensified the trend of widespread prevalence of CR-EC, and medical personnel should closely monitor the changes of dominant clone groups (such as ST410) to prevent and control the further spread of CR-EC.
Comparative Clinical Outcomes of Irrigation Techniques in Burr-Hole Craniostomy for Chronic Subdural Hemorrhage: A Multicenter Cohort Study
Authors: Cho Ho Seong , Lee Hyun-Seok , Jeon Yoo Sung , Lee Won Hee , Cho Kyung Rae 
Published: April 2024

Abstract

Background
CSDH is often treated with surgical blood drainage, but concerns about recurrence and outcomes persist. Surgical techniques, including irrigation, vary. This study compares the outcomes of irrigation in cSDH surgery.
Materials and methods
From September 2020 to September 2022, 92 cSDH patients underwent surgery. Two different irrigation methods were used: extensive irrigation (IG) and non-irrigation (NIG). Method of irrigation was selected by each surgeon’s preference. Parameters measured included volume of hematoma changes, midline shifting, complications, and basic demographics. Recurrence was defined as symptomatic or hematoma expansion more than double the volume before surgery. Factors predicting recurrence and irrigation method impact were analyzed.
Results
Eleven patients were excluded because of bilateral or related to other disease. We analyzed 81 patients (44 NIG, 37 IG). Recurrence occurred in 6 IG cases (16.2%) and 1 NIG case (2.3%). Irrigation method significantly affected recurrence (P = 0.043). Age, gender, medication, medical history, and preoperative measurements had no major impact on recurrence. NIG had unexpected cases of intracerebral hemorrhage.
Conclusion
Extensive irrigation may increase recurrence in cSDH drainage. Non-irrigation drainage had fewer recurrences, but unexpected complications arose. Careful drainage in non-irrigated cases is crucial.