bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel stability criteria of Cohen–Grossberg neural networks with time‐varying delays
Authors: Zheng Cheng‐De , Shan Qi‐He , Wang Zhanshan 
Published: August 2010

SUMMARYIn this paper, a class of Cohen–Grossberg neural networks with time‐varying delays is investigated. Based on several new Lyapunov–Krasovskii functionals, by employing the homeomorphism mapping principle, the Halanay inequality, a nonlinear measure approach and linear matrix inequality techniques, several delay‐independent sufficient criteria are obtained for the existence, uniqueness and globally exponential stability of considered neural networks. Without assuming the boundedness and monotonicity of activation functions, the obtained conditions generalize some previous results in the literature. Two examples are also given to show the less conservativeness of the obtained conditions. Copyright © 2010 John Wiley & Sons, Ltd.
Design approach for high‐bandwidth low‐power three‐stage operational amplifiers
Authors: Pugliese A. , Amoroso F. A. , Cappuccino G. , Cocorullo G. 
Published: August 2010

SUMMARYA new design approach to optimize the frequency compensation network of three‐stage operational amplifiers (op‐amps) is presented.The proposed criterion is aimed at maximizing the bandwidth of well‐established three‐stage op‐amps using Nested‐Miller Compensation with feedforward tranconductance stage and nulling resistor (NMCFNR). As shown by design examples in a commercial 0.35‐µm CMOS technology, the proposed approach allows the amplifier bandwidth to be enhanced significantly with respect to that resulting from using existing design strategies for NMCFNR op‐amps.It is also demonstrated that NMCFNR op‐amps, designed according to the proposed method, even guarantee larger values of the gain‐bandwidth product than three‐stage amplifiers using more complicated frequency compensation techniques, such as AC boosting compensation or damping‐factor control frequency compensation. Copyright © 2010 John Wiley & Sons, Ltd.
Improved detection of nicotinamide adenine dinucleotide phosphate oscillations within human neutrophils
Authors: Clark Andrea J. , Romero Roberto , Petty Howard R. 
Published: August 2010

AbstractKinetic studies of nicotinamide adenine dinucleotide phosphate autofluorescence have been conducted in adherent neutrophils using an improved microscopic photometry system incorporating low noise excitation and detection systems. Dynamic autofluorescence oscillations were found with periods ranging from ∼4 min to ∼10 s. The largest portion of the population of oscillating neutrophils (32%) had periods near 2 min. The next largest group at 25% exhibited periods of 1 min or less. These oscillations could not be accounted for by instrument artifacts, cell shape changes away from the focal plane, or other factors. They disappeared when detergent was added to oscillating cells. Higher‐frequency oscillations disappeared as cells changed shape, indicating a correlation between these two processes. This approach provides a reliable method to monitor this cellular property. © 2010 International Society for Advancement of Cytometry
An efficient identity‐based protocol for private matching
Authors: Zhong Sheng , Chen Tingting 
Published: August 2010

AbstractAn important application of the Internet is that people can find partners satisfying their requirements from the huge number of users. In this paper, we present a cryptographic protocol for private matching, in which a user finds her desired partners without revealing her requirements, and the potential partners do not need to reveal their profiles. An additional advantage of our protocol is that it is identity‐based, which means the involved parties do not need to have a priori knowledge of each other's public keys; as long as they know each other's identities, the protocol can be executed. Copyright © 2010 John Wiley & Sons, Ltd.
Support of multiple content variants in the multimedia broadcast/multicast service
Authors: Xylomenos George , Katsaros Konstantinos , Tsakanikas Vasilis 
Published: August 2010

AbstractThe Multimedia Broadcast/Multicast Service (MBMS) was designed to enable the mass distribution of multimedia content in 3rd Generation and beyond cellular networks. If such services are to become commercially viable, they must be able to efficiently support widely heterogeneous user requirements, for example, due to terminal limitations and cost constraints. This paper presents an MBMS extension that allows multiple variants of the same content to be economically distributed to heterogeneous receivers, explicitly taking into account the possibility of using either dedicated or common radio channels. We describe our extended multiple content variant MBMS model by explaining the modifications that it imposes on the standard MBMS model, as well as the manner in which it can be combined with layered coding. We also present an analytical evaluation of our approach against alternatives based on the standard MBMS in terms of control and user plane overhead, and compare the analytical predictions with detailed simulation results. Both the analysis and the simulations indicate that our proposal can indeed satisfy heterogeneous user requirements, while consuming considerably lower resources than the standard‐based alternatives. Copyright © 2010 John Wiley & Sons, Ltd.
Essential genes for astroglial development and axon pathfinding during zebrafish embryogenesis
Authors: Barresi Michael J.F. , Burton Sean , Dipietrantonio Kristina , Amsterdam Adam , Hopkins Nancy , Karlstrom Rolf O. 
Published: August 2010

AbstractThe formation of the central nervous system depends on the coordinated development of neural and glial cell types that arise from a common precursor. Using an existing group of zebrafish mutants generated by viral insertion, we performed a “shelf‐screen” to identify genes necessary for astroglial development and axon scaffold formation. We screened 274 of 315 viral insertion lines using antibodies that label axons (anti‐Acetylated Tubulin) and astroglia (anti‐Gfap) and identified 25 mutants with defects in gliogenesis, glial patterning, neurogenesis, and axon guidance. We also identified a novel class of mutants affecting radial glial cell numbers. Defects in astroglial patterning were always associated with axon defects, supporting an important role for axon‐glial interactions during axon scaffold development. The genes disrupted in these viral lines have all been identified, providing a powerful new resource for the study of axon guidance, glio‐ and neurogenesis, and neuron‐glial interactions during development of the vertebrate CNS. Developmental Dynamics 239:2603–2618, 2010. © 2010 Wiley‐Liss, Inc.
Expression, functional, and structural analysis of proteins critical for otoconia development
Authors: Xu Yinfang , Zhang Hui , Yang Hua , Zhao Xing , Lovas Sándor , Lundberg Yunxia (Yesha) Wang 
Published: August 2010

AbstractOtoconia, developed during late gestation and perinatal stages, couple mechanic force to the sensory hair cells in the vestibule for motion detection and bodily balance. In the present work, we have investigated whether compensatory deposition of another protein(s) may have taken place to partially alleviate the detrimental effects of Oc90 deletion by analyzing a comprehensive list of plausible candidates, and have found a drastic increase in the deposition of Sparc‐like 1 (aka Sc1 or hevin) in Oc90 null versus wt otoconia. We show that such up‐regulation is specific to Sc1, and that stable transfection of Oc90 and Sc1 full‐length expression constructs in NIH/3T3 cells indeed promotes matrix calcification. Analysis and modeling of Oc90 and Sc1 protein structures show common features that may be critical requirements for the otoconial matrix backbone protein. Such information will serve as the foundation for future regenerative purposes. Developmental Dynamics 239:2659–2673, 2010. © 2010 Wiley‐Liss, Inc.
Zebrafish notch signalling pathway mutants exhibit trunk vessel patterning anomalies that are secondary to somite misregulation
Authors: Therapontos Christina , Vargesson Neil 
Published: August 2010

AbstractThe Notch signalling pathway mutants, after‐eight (aei), beamter (bea), and deadly‐seven (des) have previously been used to study somitogenesis and neurogenesis. Notch signalling has also been shown to have roles in vascular development. However, vascular development in each of these three Notch mutants has not been described, and so their potential usefulness for further understanding the role of Notch signalling in angiogenesis is unknown. Here we demonstrate each of the mutants also exhibit vascular defects in inter‐somitic vessel (ISV) positioning and patterning. Ectopic filopodia were also observed on the ISVs of the mutants. Ectopic filopodia are not due to loss of dll4. Somite expression of known vascular guidance cues, efnb2, sema3a2, and plexinD1 are disrupted, suggesting that the ISV vascular phenotype is due to disruption of these cues. Developmental Dynamics 239:2761–2768, 2010. © 2010 Wiley‐Liss, Inc.
Sox9‐expressing precursors are the cellular origin of the cruciate ligament of the knee joint and the limb tendons
Authors: Soeda Tsunemitsu , Deng Jian Min , de Crombrugghe Benoit , Behringer Richard R. , Nakamura Takashi , Akiyama Haruhiko 
Published: August 2010

AbstractSox9 expression defines cell progenitors in a variety of tissues during mouse embryogenesis. To establish a genetic tool for cell‐lineage tracing and gene‐function analysis, we generated mice in which the CreERT2 gene was targeted to the endogenous mouse Sox9 locus. In Sox9CreERT2/+;R26R embryos, tamoxifen activated Cre recombinase exclusively in Sox9‐expressing tissues. To determine the suitability of this mouse line for developmental stage‐specific gene recombination, we investigated the cellular origins of the cruciate ligaments of the knee joint and the limb tendons, in which precursor cells have not been defined. The cells in these tissues were labeled after tamoxifen treatment before or at the stage of chondrogenic mesenchymal condensation, indicating that ligament and tendon cells originated from Sox9‐expressing cells and that cell fate determination occurred at mesenchymal condensation. This mouse line is a valuable tool for the temporal genetic tracing of the progeny of, and inducible gene modification in Sox9‐expressing cells. genesis 48:635–644, 2010. © 2010 Wiley‐Liss, Inc.
Self‐Assembly and the Properties of a Highly Oriented Hierarchical Nanobelt–Nanoprism Array of Ternary Oxide Zn–In–O
Authors: Pan Nan , Xue Haizhou , Huang Jinhua , Zhang Guanghui , Wu Yukun , Li Ming , Wang Xiaoping , Hou Jianguo 
Published: August 2010

AbstractA highly oriented, well‐aligned hierarchical Zn–In–O nanobelt–nanoprism array was synthesized by vapor‐phase transport and condensation using GaN epilayer as the substrate. The upper nanobelts are found to be ZnO:In with an average Zn/In molar ratio of approximately 9:1, and the subjacent nanoprisms are found to be In2O3(ZnO)m (m = 2, 3, 4, and 5) structures. During self‐assembly, the nanoprisms are vertically grown on GaN and well aligned along the superlattice stacking direction, upon which each nanobelt is horizontally grown along its [10–10] direction. The spatially resolved cathodoluminescence spectra collected on individual hierarchical nanostructures clearly show characteristics for the ZnO:In nanobelt and for the In2O3(ZnO)m nanoprism, which are distinctly different from the emissions of the undoped ZnO nanorod. I‐V measurements on individual nanobelts reveal good conductivity over 103 S m–1 and high electron concentration of 1019–1020 cm–3. This bottom‐up self‐assembled, highly oriented, and well‐aligned hierarchical nanoarray may find applications in newly emerging vertically integrated nanoarray circuits.
Novel Bi‐ and Trinuclear Gallium Halides and Hydrides with Acyclic and Bicyclic Guanidinate Substituents: Synthesis and Reactivity
Authors: Rudolf Daniel , Kaifer Elisabeth , Himmel Hans‐Jörg 
Published: August 2010

AbstractWe report on the synthesis and characterization of new molecular Ga halides and hydrides with acyclic guanidinate substituents with bicyclic guanidinate substituents. Acyclic guanidinates were found to adopt terminal bonding modes like in the dimeric GaII compound [(iPr2N)C(NiPr)2GaI]2. In contrast, bicyclic guanidinates prefer bridging bonding modes. Hence, the reaction between Me3N·GaH3 and htbo (1,4,6‐triazabicyclo[3.3.0]oct‐4‐ene) affords the binuclear GaIII hydride [H2Ga(μ‐tbo)]2. This new hydride turned out to be unstable in solution at 25 °C, dihydrogen is slowly eliminated. In the solid state, however, the hydride is stable up to 80 °C. The thermodynamic properties of this and similar dehydrogenation reactions were studied my means of quantum chemical calculations. With Ga2H5(μ3‐O)(μ‐tbn)2 and Ga2H5(μ3‐O)(μ‐hpp)2, two new hydrides were synthesized which can be regarded as the first hydrolysis intermediates of binuclear Ga hydrides with bridging guanidinate substituents.
The Participation of 2H‐Pyran‐2‐ones in [4+2] Cycloadditions: An Experimental and Computational Study
Authors: Štefane Bogdan , Perdih Andrej , Pevec Andrej , Šolmajer Tomaž , Kočevar Marijan 
Published: August 2010

AbstractExperimental and computational DFT studies of the Diels–Alder (DA) reaction mechanism for the series of 2H‐pyran‐2‐ones 1 and non‐symmetrically substituted alkynes 2, 10, and 11 were undertaken. Several 2H‐pyran‐2‐ones 1 were treated with N,N‐diethylpropynamine (2). The results point toward a polar and asynchronous two‐step mechanism that is in line with the characteristics of a polar Diels–Alder (P‐DA) reaction mechanism.5 Unexpected formation of minor products 4a–d during the reaction of 1 with 2 can be rationalized by considering the easily passable torsion barrier around the newly formed C–C bond that enables two different cyclization modes. The charge transfer (CT) and global reactivity indices analyses demonstrated that in these reactions 2H‐pyran‐2‐ones act as electrophiles and N,N‐diethylpropynamine (2) acts as a nucleophile reaction partner. The introduction of electron‐withdrawing carboxylic substituents on the dienophiles 10 and 11 shifts the DA reaction with 2H‐pyran‐2‐ones to a less polar and more synchronous concerted reaction mechanism. Calculated CT as well as values of the global electrophilicity and nucleophilicity N indexes revealed that 2H‐pyran‐2‐ones act in this case as nucleophiles and reacting dienophiles 10 and 11 as electrophiles. The results are consistent with the experimental data and kinetic analyses, thus providing valuable insights into 2H‐pyran‐2‐one chemistry and paving the way for an efficient synthesis of highly functionalized benzene derivatives as well as broadening the mechanistic understanding of these highly versatile reactions.
Enantiodivergent Chemoenzymatic Synthesis of 4‐Hydroxypiperidine Alkaloids
Authors: Bartali Laura , Casini Andrea , Guarna Antonio , Occhiato Ernesto G. , Scarpi Dina 
Published: August 2010

AbstractAn efficient chemoenzymatic synthesis of both enantiomers of fagomine, as well as ofcisandtrans‐4‐hydroxypipecolic acid is reported. The synthesis starts from commercial δ‐valerolactam which, after a Pd‐catalyzed methoxycarbonylation of the corresponding vinyl phosphate, is subjected to allylic oxidation to give a racemic 4‐hydroxytetrahydropyridine derivative in 57 % overall yield. This product is resolved by an enzyme‐catalyzed esterification using immobilized lipases fromCandida antarctica(Novozym 435) andBurkholderia cepacia(lipase PS Amano IM). The latter provides the correspondingResters and theSalcohol in 95 and 94 %ee, respectively. TheSalcohol is then converted intoL‐fagomine by a stereoselective hydroboration/oxidation as key steps and thecis‐(2R,4S)‐4‐hydroxypipecolic acid by stereoselective hydrogenation. The correspondingD‐fagomine andcis‐(2S,4R)‐4‐hydroxypipecolic acid, as well astrans‐(2R,4R)‐4‐hydroxypipecolic acid can be prepared by the same strategy after hydrolysis of theRester obtained by kinetic resolution.
Some Possibilities and Limitations of Contactless Impedimetric Determinations of Organic Liquids in Aqueous Solutions. The Interference from Ionic Compounds
Authors: Opekar František , Štulík Karel , Fišarová Michaela 
Published: August 2010

AbstractThe possibilities of impedimetric determinations of organic liquids in mixtures with aqueous solutions have been studied in detail, using a planar capacitor cell combined with multivibrator and operational amplifier circuits functioning as a differentiator and a system of dioxane with aqueous solutions of alkali halides. The experimental values have been compared with a simple model and the detector response has been characterized. The behaviour of the detection cell can be reliably predicted on this basis. This approach permits extremely simple, rapid and cheap analyses of binary liquid mixtures, however, it suffers from a serious limitation in that the overall electric conductivity of the mixture must be low.
The Signal‐Enhanced Label‐Free Immunosensor Based on Assembly of Prussian Blue‐SiO2 Nanocomposite for Amperometric Measurement of Neuron‐Specific Enolase
Authors: Zhong Zhaoyang , Shan Jinlu , Zhang Zhiming , Qing Yi , Wang Dong 
Published: August 2010

AbstractA signal‐enhanced label‐free electrochemical immunosensor was constructed by the employment of Prussian blue doped silica dioxide (PB‐SiO2) nanocomposite. At first, PB‐SiO2 nanocomposite which was produced by using a microemulsion method was used to obtain a nanostructural monolayer on a glassy carbon electrode (GCE) surface. Next amino‐functionalized interface were prepared by self‐assembling 3‐aminopropyltriethoxy silane (APTES) on the PB‐SiO2 nanoparticle surface. Then chitosan stabled gold nanoparticle (CS‐nanoAu) was subsequently attached, while the entire surface was finally loaded with neuron‐specific enolase antibody (anti‐NSE) via the adsorption of gold nanoparticle. The sensitivity of the proposed immunosensor has greatly improved as the PB‐SiO2 nanostructural sensing film provides plenty of active sites which might catalyze the reduction of H2O2. The immunosensor exhibited good linear behavior in the concentration range from 0.25–5.0 and 5.0–75 ng/mL for the quantitative analysis of neuron‐specific enolase (NSE), a putative serum marker of small‐cell lung carcinoma (SCLC), with a limit of detection of 0.08 ng/mL. The resulting NSE immunosensor showed high sensitivity and long‐term lifetime which can be attributed to the extremely high catalytic activity and biocompatibility of CS‐nanoAu/APTES/PB‐SiO2 nanostructural multilayers.
Development of a Xanthine Oxidase Modified Amperometric Electrode for the Determination of the Antioxidant Capacity
Authors: Cortina‐Puig Montserrat , Scangas Anastasia C. H. , Marchese Zachary S. , Andreescu Silvana , Marty Jean‐Louis , Calas‐Blanchard Carole 
Published: August 2010

AbstractThis paper describes the development of a xanthine oxidase/poly‐m‐phenylenediamine (XOD‐PPD)‐modified electrode. The biosensor was constructed by encapsulating XOD in a sol‐gel matrix deposited onto a platinum based screen‐printed electrode functionalized with a permselective PPD membrane. The hydrogen peroxide generated as a final product of the enzymatic reaction between the hypoxanthine and the XOD or by the spontaneous dismutation of superoxide radicals was selectively monitored at +700 mV. The use of a highly selective PPD layer blocked the nonspecific oxidation of other oxidizable molecules. Finally the biosensor was applied to the determination of the antioxidant capacity of acetylsalicylic acid.
Gold Nanoparticles Electrodeposited on Ordered Mesoporous Carbon as an Enhanced Material for Nonenzymatic Hydrogen Peroxide Sensor
Authors: Wang Lixia , Bo Xiangjie , Bai Jing , Zhu Liande , Guo Liping 
Published: August 2010

AbstractA facile and controllable electrodeposition method was developed to directly attach gold nanoparticles (GNPs) on ordered mesoporous carbon (OMC). The GNPs on OMC substrate were characterized by scanning electron microscopy (SEM), X‐ray diffraction (XRD) and X‐ray photoelectron spectrometer (XPS), respectively. A nonenzymatic hydrogen peroxide (H2O2) sensor was fabricated on GNPs‐OMC/GCE. The sensor demonstrated a fast amperometric response (2.5 s), a wide linear range toward H2O2 concentrations between 2.0×10−6 and 3.92×10−3 M (R=0.999), and a low detection limit of 0.49 µM (S/N=3). Moreover, it exhibited good reproducibility and long‐term stability. The excellent electrocatalytical activity might be attributed to the synergistic effect of OMC and GNPs.
Polyaniline/Single‐Walled Carbon Nanotubes Composite Based Triglyceride Biosensor
Authors: Dhand Chetna , Solanki Pratima R. , Datta Monika , Malhotra B. D. 
Published: August 2010

AbstractNanocomposite film comprising of polyaniline (PANI) and single walled carbon nanotubes (SWCNT) has been fabricated onto indium‐tin‐oxide (ITO) coated glass plate using electrophoretic technique. Co‐immobilization of glycerol dehydrogenase (GDH) and lipase (LIP) has been done via N‐ethyl‐N′‐(3‐dimethylaminopropyl) carbodiimide and N‐hydroxysuccinimide chemistry to explore its application for triglyceride (tributyrin) sensing. Response studies have been done using linear sweep voltammetry revealing that LIP‐GDH/PANI‐SWCNT‐TB/ITO bioelectrode can detect tributyrin in the range of 50 to 400 mg dL−1 with low Michaelis–Menten constant of 1.138 mM, improved response time of 12 s, high sensitivity as 4.28×10−4 mA mg−1 dL and storage stability of about 13 weeks.
Study of electromigration effects on a pH boundary during the on‐line electrokinetic preconcentration by capillary electrophoresis
Authors: Vítková Kateřina , Petr Jan , Maier Vítězslav , Znaleziona Joanna , Ševčík Juraj 
Published: August 2010

AbstractA contribution to the description of electrokinetic effects on the pH boundary formed by sodium borate pH 9.5 and sodium phosphate pH 2.5 electrolytes for on‐line preconcentration of weak acids is presented in this article. Simulations of electrokinetic injections together with experimental studies using contactless conductivity detection verified that the preconcentration is induced mainly by dissociation changes of analytes on the pH boundary and transient ITP state. Moreover, a study of the addition of organic solvent to the injection electrolyte was performed with impressive results. Subnanomolar LODs of hydroxybenzoic acids were achieved with 80% of methanol in the injection electrolyte which represents more than 70 000‐fold preconcentration in comparison with classical CZE method.
Parallel separations using capillary electrophoresis on a multilane microchip with multiplexed laser‐induced fluorescence detection
Authors: Nikcevic Irena , Piruska Aigars , Wehmeyer Kenneth R. , Seliskar Carl J. , Limbach Patrick A. , Heineman William R. 
Published: August 2010

AbstractParallel separations using CE on a multilane microchip with multiplexed LIF detection is demonstrated. The detection system was developed to simultaneously record data on all channels using an expanded laser beam for excitation, a camera lens to capture emission, and a CCD camera for detection. The detection system enables monitoring of each channel continuously and distinguishing individual lanes without significant crosstalk between adjacent lanes. Multiple analytes can be determined in parallel lanes within a single microchip in a single run, leading to increased sample throughput. The pKa determination of small molecule analytes is demonstrated with the multilane microchip.