bluuebunny/crossref_metadata_2025_split huggingface dataset:
Design of chiral acidic molecularly imprinted polymer for the enantioselective separation of (±)‐DOPA
Authors: Alnoman Rua B 
Published: June 2024

AbstractBackgroundThe study focuses on developing a stable enantioselective matrix for the efficient chiral identification and enantioresolution of l‐DOPA and (±)‐DOPA. The matrix is derived from a copolymeric material made from poly[(vinylsulfonic acid)‐co‐(4‐vinylpyridine)] crosslinked with divinylbenzene.Resultsl‐DOPA‐vinylsulfonamide was synthesized and characterized. This chiral sulfonamide was copolymerized with 4‐vinylpyridine in the presence of a divinylbenzene crosslinker, using azobisisobutyronitrile as a thermal initiator. Post‐polymerization, the polymeric particles were treated with NaOH and subsequently washed with acid to remove the integrated l‐DOPA species. Scanning electron microscopy and Fourier transform infrared spectroscopy confirmed the imprinted l‐DOPA‐IP particles. The manufactured l‐DOPA‐IP demonstrated a tenfold greater affinity for l‐DOPA compared to d‐DOPA. Langmuir adsorption experiments at pH 6 showed a maximum capacity of 162 mg g−1. Enantiomeric excess values for l‐ and d‐DOPA, determined through optical separation using a column approach, were 94% and 82%, respectively, in the loading and recovery solutions.ConclusionThe developed copolymeric material effectively served as an enantioselective matrix, exhibiting high selectivity and capacity for l‐DOPA. The process demonstrated efficient chiral identification and separation, achieving significant enantiomeric excess values for both l‐ and d‐DOPA. © 2024 Society of Chemical Industry (SCI).
Insights on agricultural nitrate leaching from soil block mesocosms
Authors: Loper Holly , Tenesaca Carlos , Pederson Carl , Helmers Matthew J. , Crumpton William G. , Lemke Dean , Hall Steven J. 
Published: June 2024

AbstractQuantifying nitrate leaching in agricultural fields is often complicated by inability to capture all water draining through a specific area. We designed and tested undisturbed soil monoliths (termed “soil block mesocosms”) to achieve complete collection of drainage. Each mesocosm measures 1.5 m × 1.5 m × 1.2 m and is enclosed by steel on the sides and bottom with a single outlet to collect drainage. We compared measurements from replicate mesocosms planted to corn (Zea mays L.) with a nearby field experiment with tile‐drained plots (“drainage plots”), and with drainage from nearby watersheds from 2020 through 2022 under drought conditions. Annual mesocosm drainage volumes were 6.5–24.6 cm greater than from the drainage plots, likely because the mesocosms were isolated from the subsoil and could not store groundwater below the drain depth, whereas the drainage plots accumulated infiltration as groundwater. Thus, we obtained consistent nitrate leaching measurements from the mesocosms even when some drainage plots yielded no water. Despite drainage volume differences, mean flow‐weighted nitrate concentrations were similar between mesocosms and drainage plots in 2 of 3 years. Mesocosm annual drainage volume was 8.7 cm lower to 16.7 cm higher than watershed drainage, likely due to lagged influences of groundwater. Corn yields were lower in mesocosms than drainage plots in 2020, but with irrigation, yields were similar in subsequent years. Mean 2020 surface soil moisture and temperature were similar between the mesocosms and nearby fields. Based on these comparisons, the mesocosms provide a robust method to measure nitrate leaching with lower variability than field plots.
Exploring genetic counselors' practice of discussing clinical trials with patients
Authors: Bloom Thea , Bonini Katherine E. , Gutierrez‐Kapheim Melissa , Kinsley Lisa M. , Smith Maureen E. , Duquette Debra 
Published: June 2024

AbstractDespite concerted and accelerated efforts to increase the knowledge of medicine and disease via clinical studies, clinical trials continue to face low enrollment for all patient groups. The dissemination of the availability of clinical trials to individuals with or at risk for hereditary disorders is critical. This study acts as a foundation in determining an unexplored role of clinical trial discussion in genetic counseling practice. Board‐certified, patient‐facing genetic counselors in the United States were invited to participate in an anonymous survey via the National Society of Genetic Counselors. Between February and April 2022, 157 participants (N = 157) completed the survey on clinical trial discussion with patients, barriers, and facilitators to discussing clinical trials with patients, research experience, and demographics. Survey results identified that most respondents have discussed the availability of clinical trials with a patient (85%). Almost one‐third have previous research experience working for a clinical trial (30%). Most agreed that discussions of clinical trials are within the scope of genetic counseling (82%); however, one‐third were not comfortable discussing them with patients (34%). Respondents who know how to find specific clinical trials (p < 0.001) were reportedly more likely to be comfortable discussing clinical trials with their patients. In addition to clinical research exposure, this study suggests that further education and training is necessary for genetic counselors to learn how to find and identify specific clinical trials for their patients. In turn, we hope for this to increase genetic counselors' comfort of clinical trial discussion.
The role of ultraviolet radiation in the flavor formation during drying processing of Pacific saury (Cololabis saira)
Authors: Zhao Ling , Wang Lin , Cao Rong , Liu Qi , Su Dong , Zhang Yating , Yu Yueqin 
Published: June 2024

AbstractBACKGROUNDTraditional sun‐drying aquatic products are popular and recognized by customers, owing to their unique flavor and long‐term preservation. However, the product quality and production efficiency cannot be guaranteed. Cololabis saira is rich in unsaturated fatty acids, which are susceptible to hyperoxidation during the drying process. This study aimed to make clear the role of ultraviolet (UV) radiation in flavor formation during drying processes of Cololabis saira to develop a modern drying technology.RESULTSLipid oxidation analysis revealed that moderate hydrolytic oxidation occurred in the UV‐assisted cold‐air drying group due to the combined influence of UV and cold‐air circulation, resulting in the thiobarbituric acid reactive substances value being higher than that of cold‐air drying group but lower than the natural drying group. Hexanal, heptanal, cis‐4‐heptenal, octanal, nonanal, (trans,trans)‐2,4‐heptadienal, (trans,trans)‐2,6‐nonanedial, 1‐octen‐3‐ol, heptanol, 2,3‐pentanedione, 3,5‐octadien‐2‐one and trimethylamine were identified as the characteristic flavor odor‐active compounds present in all Cololabis saira samples. Yet, during the natural drying process, sunlight promoted the lipid oxidation, resulting in the highest degree of lipid oxidation among three drying methods. Light and heat promoted lipid oxidation in Cololabis saira prepared through natural drying process, leading to a large accumulation of volatile compounds, such as 3‐methylbutyraldehyde, 2,3‐pentanedione, 1‐propanol, and 3‐pentanone. Cold air circulation inhibited lipid oxidation to some extent, resulting in a blander flavor profile. More cis‐4‐heptenal, cis‐2‐heptenal, octanal and 2‐ethylfuran accumulated during the UV‐assisted cold‐air drying process, enriching its greasy flavor and burnt flavor.CONCLUSIONUV‐assisted cold‐air drying could promote moderate lipid oxidation, which is beneficial for improving product flavor. To sum up, UV radiation played a crucial role in the flavor formation during the drying process of Cololabis saira. © 2024 Society of Chemical Industry.
Dose‐dependent protective effects of Lactobacillus rhamnosus GG against stress‐induced ulcer
Authors: Işık Musab , Özbayer Cansu , Dönmez Dilek Burukoğlu , Erol Kevser , Çolak Ertuğrul , Üstüner Mehmet Cengiz , Değirmenci İrfan 
Published: June 2024

AbstractBACKGROUNDStress‐related diseases are on the rise and stress is one of the common factors that lead to ulcer. Stress‐induced mucosal bleeding is a serious complication observed in many critically ill patients. Due to the harmful side effects of proton pump inhibitors, natural and active alternative treatment methods for peptic ulcer treatment that are safe in terms of side effects are an urgent need for human health. We aimed to investigate the dose‐dependent protective effects of Lactobacillus rhamnosus GG (LGG) against stress ulcer induced by cold restraint stress in rats. This study was performed in a total of 42 rats, in control group (C), stress group (S), pantoprazole (20 mg kg−1 day−1) group (P), LGG (3 × 108 cfu mL−1 day−1) + stress group (M1), LGG (15 × 108  mL−1 day−1) + stress group (M5) and LGG (30 × 108  mL−1 day−1) + stress group (M10) (each n = 7). Ulceration areas (mm2) were determined quantitatively with ImageJ software. Glucocorticoid, catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) levels were determined by ELISA and malondialdehyde levels were determined by spectrophotometric measurement. Histopathological examinations were performed in gastric tissue.RESULTSTherapeutic dose of LGG increased CAT, SOD and GPx levels; prevented excessive activation of the hypothalamic–pituitary–adrenal axis; reduced ulceration and bleeding in the gastric mucosal layer; and provided stabilization of mast cells.CONCLUSIONSWe can suggest that LGG may be beneficial for reducing the negative effects of stress on the body, for protecting against ulcer disease and for reducing or preventing the risk of stress‐induced gastrointestinal bleeding in patients staying in intensive care units. © 2024 The Author(s). Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Outcomes of Percutaneous Endoscopic Gastrostomy in Huntington's Disease at a Tertiary Center
Authors: Farag Mena , Coleman Annabelle , Knights Harry , Murphy Michael J. , Rajagopal Sangeerthana , Touzé Alexiane , Shoai Maryam , Hearst Cara , Salanio Desiree M. , Wild Edward J. , Tabrizi Sarah J. 
Published: June 2024

AbstractBackgroundClinically assisted nutrition and hydration via percutaneous endoscopic gastrostomy (PEG) is a therapeutic option to ameliorate the difficulties associated with enhanced catabolism, weight loss, and dysphagia in Huntington's disease (HD).ObjectivesThe objective is to provide insights into demographics, staging (Shoulson‐Fahn), complications, weight trajectories, and survival rates in people with HD (pwHD) who underwent PEG.MethodsThis retrospective study included 705 consecutive pwHD who attended our HD clinic between July 2006 and March 2024, of whom 52 underwent PEG. A control group (n = 52), comprising pwHD without PEG, were closely matched for sex, stage, age, CAG length, and disease burden score at PEG. The study was registered as a service evaluation at the National Hospital for Neurology and Neurosurgery.ResultsPEG prevalence was 15.0% (n = 52/347) among manifest pwHD: 4.8% (n = 3/62) for Stage 3; 33.3% (n = 16/48) for stage 4; and 44.1% (n = 30/68) for stage 5. Commonest indications were dysphagia, weight loss, and inadequate oral intake. Complications included chest infection, tube dislodgement, and peristomal and skin infections. Modeling of weight trajectories after PEG found no difference between PEG and non‐PEG groups. Mortality rate was 34.6% (n = 18/52) in the PEG and 36.5% (n = 19/52) in the non‐PEG groups (P = 0.84). Treatment duration (until study endpoint or death) was 3.48 years (interquartile range = 1.71–6.02; range = 0.23–18.8), with 65.4% (n = 34/52) alive at the study endpoint.ConclusionPEG in pwHD at‐risk for weight loss may help slow weight loss. Prospective studies are required to strengthen PEG decision‐making in pwHD. PEG survival was much longer than other dementias, highlighting the need to consider PEG independently in pwHD.
Structural analysis of neomycin B and kanamycin A binding Aminoglycosides Modifying Enzymes (AME) and bacterial ribosomal RNA
Authors: Revillo Imbernon Julia , Weibel Jean‐Marc , Ennifar Eric , Prévost Gilles , Kellenberger Esther 
Published: June 2024

AbstractAminoglycosides are crucial antibiotics facing challenges from bacterial resistance. This study addresses the importance of aminoglycoside modifying enzymes in the context of escalating resistance. Drawing upon over two decades of structural data in the Protein Data Bank, we focused on two key antibiotics, neomycin B and kanamycin A, to explore how the aminoglycoside structure is exploited by this family of enzymes. A systematic comparison across diverse enzymes and the RNA A‐site target identified common characteristics in the recognition mode, while assessing the adaptability of neomycin B and kanamycin A in various environments.
Myotonia congenita in a Greek cohort: Genotype spectrum and impact of the CLCN1:c.501C > G variant as a genetic modifier
Authors: Marinakis Nikolaos M. , Svingou Maria , Papadimas Giorgos‐Konstantinos , Papadopoulos Constantinos , Chroni Elisabeth , Pons Roser , Pavlou Evangelos , Sarmas Ioannis , Kosma Konstantina , Apostolou Paraskevi , Sofocleous Christalena , Traeger‐Synodinos Joanne , Kekou Kyriaki 
Published: June 2024

AbstractIntroduction/AimsMyotonia congenita (MC) is the most common hereditary channelopathy in humans. Characterized by muscle stiffness, MC may be transmitted as either an autosomal dominant (Thomsen) or a recessive (Becker) disorder. MC is caused by variants in the voltage‐gated chloride channel 1 (CLCN1) gene, important for the normal repolarization of the muscle action potential. More than 250 disease‐causing variants in the CLCN1 gene have been reported. This study provides an MC genotype–phenotype spectrum in a large cohort of Greek patients and focuses on novel variants and disease epidemiology, including additional insights for the variant CLCN1:c.501C > G.MethodsSanger sequencing for the entire coding region of the CLCN1 gene was performed. Targeted segregation analysis of likely candidate variants in additional family members was performed. Variant classification was based on American College of Medical Genetics (ACMG) guidelines.ResultsSixty‐one patients from 47 unrelated families were identified, consisting of 51 probands with Becker MC (84%) and 10 with Thomsen MC (16%). Among the different variants detected, 11 were novel and 16 were previously reported. The three most prevalent variants were c.501C > G, c.2680C > T, and c.1649C > G. Additionally, c.501C > G was detected in seven Becker cases in‐cis with the c.1649C > G.DiscussionThe large number of patients in whom a diagnosis was established allowed the characterization of genotype–phenotype correlations with respect to both previously reported and novel findings. For the c.501C > G (p.Phe167Leu) variant a likely nonpathogenic property is suggested, as it only seems to act as an aggravating modifying factor in cases in which a pathogenic variant triggers phenotypic expression.
Brain metabolism after therapeutic hypothermia for murine hypoxia‐ischemia using hyperpolarized [1‐13C] pyruvate magnetic resonance spectroscopy
Authors: Liu Xiaodan , Manninen Tiina , Capper Alkisti Mikrogeorgiou , Jiang Xiangning , Ellison Jacob , Kim Yaewon , Gurler Gokce , Xu Duan , Ferriero Donna M. 
Published: June 2024

AbstractHypoxic‐ischemic encephalopathy (HIE) is a common neurological syndrome in newborns with high mortality and morbidity. Therapeutic hypothermia (TH), which is standard of care for HIE, mitigates brain injury by suppressing anaerobic metabolism. However, more than 40% of HIE neonates have a poor outcome, even after TH. This study aims to provide metabolic biomarkers for predicting the outcomes of hypoxia‐ischemia (HI) after TH using hyperpolarized [1‐13C] pyruvate magnetic resonance spectroscopy. Postnatal day 10 (P10) mice with HI underwent TH at 1 h and were scanned at 6–8 h (P10), 24 h (P11), 7 days (P17), and 21 days (P31) post‐HI on a 14.1‐T NMR spectrometer. The metabolic images were collected, and the conversion rate from pyruvate to lactate and the ratio of lactate to pyruvate in the injured left hemisphere (kPL(L) and Lac/Pyr(L), respectively) were calculated at each timepoint. The outcomes of TH were determined by the assessments of brain injury on T2‐weighted images and behavioral tests at later timepoint. kPL(L) and Lac/Pyr(L) over time between the good‐outcome and poor‐outcome groups and across timepoints within groups were analyzed. We found significant differences in temporal trends of kPL(L) and Lac/Pyr(L) between groups. In the good‐outcome group, kPL(L) increased until P31 with a significantly higher value at P31 compared with that at P10, while the level of Lac/Pyr(L) at P31 was notably higher than those at all other timepoints. In the poor‐outcome group, kPL(L) and Lac/Pyr(L) increased within 24 h. The kPL(L) value at P11 was considerably higher compared with P10. Discrete temporal changes of kPL(L) and Lac/Pyr(L) after TH between the good‐outcome and poor‐outcome groups were seen as early as 24 h after HI, reflecting various TH effects on brain anaerobic metabolism, which may provide insights for early screening for response to TH.
An efficient flux‐variable approximation scheme for Darcy's flow
Authors: Adhikari Rajan B. , Kim Imbumn , Lee Young Ju , Sheen Dongwoo 
Published: June 2024

AbstractWe present an efficient numerical method to approximate the flux variable for the Darcy flow model. An important feature of our new method is that the approximate solution for the flux variable is obtained without approximating the pressure at all. To accomplish this, we introduce a user‐defined parameter delta, which is typically chosen to be small so that it minimizes the negative effect resulting from the absence of the pressure, such as inaccuracy in both the flux approximation and the mass conservation. The resulting algebraic system is of significantly smaller degrees of freedom, compared to the one from the mixed finite element methods or least‐squares methods. We also interpret the proposed method as a single step iterate of the augmented Lagrangian Uzawa applied to solve the mixed finite element in a special setting. Lastly, the pressure recovery from the flux variable is discussed and an optimal‐order error estimate for the method is obtained. Several examples are provided to verify the proposed theory and algorithm, some of which are from more realistic models such as SPE10.
Recent developments in the polybenzoxazine based bionanocomposites: A review
Authors: Kavitha N. , Chandramohan A. , Dinakaran K. , Prabukanthan P. 
Published: June 2024

AbstractAn overview of bionanocomposites of polybenzoxazine, which is a highly promising material, is presented in this review paper along with an update on the current developments in this material. With an enormous range of applications, polybenzoxazines are emerging as a latest set of phenolic resins. Its favorable features make it an excellent replacement for many current polymers due to its simplicity in structure design, synthetic process, and polymerization reactions. Problematic aspects of petro‐based raw materials are their nonrenewable nature, toxicity, and rising expense. Hence, the advent of sustainable benzoxazines is promising since it enables the synthesis of monomers without the need for solvents and the processing of these monomers to create extremely thermally stable thermoset resins. This is particularly true when the monomer is derived from naturally occurring, agro‐waste cardanol. A platform for additional structural alterations to expand the uses of cardanol benzoxazine in adhesives, composites, and energy storage devices is provided by its intrinsic properties. There are numerous articles are available for biobased polybenzoxazine, and hence, the focus of the present review is dedicated to (1) nanomaterails loaded bio‐based polybenzoxazines, and (2) bio‐derived nanomaterials loaded polybenzoxazines.
Low energy impact damage characteristics of epoxy coating on the surface of sandwich composites
Authors: Jia Caixia , Cui Hang , Wang Qian , Li Zhixin , Qiu Yunpeng , Yan Chao 
Published: June 2024

AbstractWhen composite honeycomb sandwich structures are subjected to low energy impacts, the upper panel or the honeycomb core often suffers large damages, which are invisible, but affects the structural safety. To achieve non‐destructive detection of these damages, an epoxy resin film (ERF) was designed as a functional coating to display the internal failure features on structure surface. The effect of low energy impacts on the damage modes of the ERF was first investigated, and then the correspondence between the ERF damage characteristics and the structural internal failure was established. Simulation and experimental results showed that with the increase of impact energy, cracks on the ERF expanded from the radial radiation with a small extension range to the circumferential pattern with an increased damage area. Meanwhile, localized delamination appeared inside the upper panel, while the degree of flexure and crushing damage to the core continued to increase. Based on the analysis of the crack extension pattern of the ERF and the damage state of the upper panel and honeycomb core, a specific link between the ERF crack characteristics and the internal damages to the sandwich structure was obtained, which might offer a novel non‐destructive testing method for composite impact damages.Highlights
Low energy impact damage in honeycomb sandwich structures was a concern.
Impact damage response of epoxy resin film on sandwich composites was studied.
Crack extension of the film showed a particular change from radial to annular.
Specific link between film crack and sandwich structure damage was established.
The results would be helpful for damage detection in sandwich composites.
3D‐printed spider web‐inspired sensor with integrated energy‐absorption and force perception capabilities
Authors: Zhang Yuhan , Li Jianyang , Wang Gexin , Zhang Yuzhe , Zhao Yao , Gong Yongqi , Li Bingqian , Wang Kunyang 
Published: June 2024

AbstractNature has evolved a myriad of dedicate‐designed structures that assist in prey capture. Spiders employ their web structures for vibrational sensing and prey capture, analogous to a component of a neurosensory system. Here, we propose a spider web‐inspired sensor with integrated energy‐absorption and force perception capabilities by 3D printing. The conductive carbon black/TPU composite made from TPU granules and carbon black particles are employed for 3D printing. We analyze the underlying sensing mechanism of conductive CB/TPU composites stemming from the enhanced dispersion of the CB particles. Through 3D printing method, we are allowed to regulate the structure's form, filling density, arrangement, and printing path to tailor the static mechanical properties, initial resistance, and force sensitivity of the spider‐web inspired structure. The static mechanical model for single silk of the spider‐web inspired structure is constructed. We explore the energy absorption capability of spider‐web inspired structures with different structural parameters, and demonstrate its impact perceiving characteristics at different falling height. Additionally, we assembled a circuit to visually reflect the falling height of the impacting ball through the brightness of the LED light. This study provides insights to endowing structural materials with intelligent characteristics, contributing to their practical applications in the fields of biomedical, soft robotics, and artificial skin, etc.Highlights
A spider web‐inspired sensor is 3D printed with CB/TPU composites.
The sensor has integrated force perception and energy‐absorption capabilities.
The sensitivity capabilities can be regulated by parameter design.
The mechanical model of the bioinspired structure is constructed.
Its impact perceiving characteristics are demonstrated by two applications.