bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sense of coherence and suicidality in suicide attempters: a prospective study
Authors: SJÖSTRÖM N. , HETTA J. , WAERN M. 
Published: May 2011

Accessible summary
The usefulness of the Sense of Coherence (SOC) scale in the nursing setting is well‐established.
The aim of this prospective clinical study was to test whether low SOC predicted future suicidality in suicide attempters.
Low SOC at index attempt was associated with suicidality at 2‐month follow‐up, and associations were independent of major depression and symptom burden.
Low SOC at index attempt was associated with repeat self‐harm during a 3‐year observation period. However, the association did not remain after adjustment for depression and symptom burden.
The SOC may help to identify patients at continued risk for suicidal behaviour.
AbstractThe usefulness of the Sense of Coherence (SOC) scale in the nursing setting is well‐established, and an association between SOC and suicidality has been suggested. The aim was to test whether low SOC at index attempt is an independent predictor of suicidality at 2‐month follow‐up and of risk for repeat attempt. The study, which had a prospective cross‐sectional design, included patients admitted to hospital after a suicide attempt. They were interviewed by means of Structured Clinical Interview for DSM‐IV. Participants (n= 155) completed the SOC scale and the Comprehensive Psychopathological Self‐rating Scale for Affective Syndromes. Suicidality was rated with the Suicide Assessment Scale. Instruments were employed again at follow‐up. Non‐fatal/fatal repetition within 3 years was determined by review of hospital records. Low SOC at baseline predicted high suicidality at follow‐up. The association remained after adjustment for major depression and affective symptom burden. Repeat attempts were made by 54 persons. Low baseline SOC was associated with repeat attempt, but the association did not remain after adjustment for major depression and symptom burden. Low SOC ratings could be a marker of risk for high suicidality in the aftermath of a suicide attempt. The SOC scale could be incorporated in nursing assessments of suicide attempters.
Ethnicity predicts perceptions of smoking and smoking cessation among veterans
Authors: KARVONEN‐GUTIERREZ C. A. , EWING L. A. , TAYLOR N. J. , ESSENMACHER C. A. , DUFFY S. A. 
Published: May 2011

Accessible summary
This study shows that, compared with White smokers, non‐White smokers treated in a primarily psychiatric Veterans Affairs hospital were more motivated to quit smoking, yet less likely to be interested in traditional cessation interventions such as intensive nurse counselling and medications.
Novel strategies such as take‐home cessation materials, Web‐based interventions and peer counselling may be needed to reach non‐White veteran smokers.
These and other strategies will be needed to address the increasing numbers of non‐Whites entering the military, especially returning Operation Enduring Freedom/Operation Iraqi Freedom veterans, many of whom smoke and have co‐morbid post‐traumatic stress disorder and traumatic head injuries making quitting smoking difficult.
AbstractThe objective of this study was to determine if race/ethnicity predicts motivation to quit smoking and preferences for cessation services among smokers serviced by a primarily psychiatric Veterans Affairs hospital. A self‐administered survey was given to a convenience sample of smokers (n= 146) at the Battle Creek Veterans Affairs Medical Center. Univariate, bivariate and multivariate regression analyses were calculated to determine the association between race/ethnicity and motivation to quit smoking. Forty‐two per cent of the sample was non‐White. Non‐White patients smoked significantly less cigarettes per day as compared with White patients (P= 0.002). In the multivariate analyses, compared with Whites, non‐Whites had 3.5 times greater odds of thinking that quitting smoking was extremely/very important to health (P= 0.01), 4.0 times greater odds of thinking of quitting using tobacco products in the next 30 days (P= 0.004) and 3.4 times greater odds of being interested in receiving smoking cessation services (P= 0.007). Yet, non‐White patients were less likely to be interested in intensive nurse counselling and cessation medications. As the number of non‐Whites continues to increase in the military, novel strategies may be needed to capitalize on the high motivation to quit smoking and preference for non‐traditional interventions among non‐White smokers treated in Veterans Affairs hospitals.
Molecular and functional identification of organic anion transporter isoforms in cultured bovine mammary epithelial cells (BME‐UV)
Authors: AL‐BATAINEH M. M. , Van Der MERWE D. , SCHULTZ B. D. , GEHRING R. 
Published: May 2011

Al‐Bataineh, M. M., van der Merwe, D., Schultz, B. D., Gehring, R. Molecular and functional identification of organic anion transporter isoforms in cultured bovine mammary epithelial cells (BME‐UV). J. vet. Pharmacol. Therap. 35, 209–215.Mammary epithelial cells express a diversity of membrane transporters including members of organic cation and organic anion (OAT) transporter subfamilies. Four mammal OAT isoforms have been identified: OAT‐1, OAT‐2, OAT‐3, and OAT‐4. The pharmacological significance of OAT isoforms has been emphasized because of their role in the movement of a wide variety of substrates across epithelial barriers. The present study identified (molecularly and functionally) bovine OAT isoforms in bovine mammary epithelial (BME‐UV) cells. mRNA expression levels of all tested transporters in BME‐UV cells were less than expression levels of the corresponding transporters in bovine kidney. Directionality in the flux of P‐aminohippuric acid and acetylsalicylate, compounds known to interact with OAT‐1 and OAT‐2, respectively, across BME‐UV monolayers was not observed at the concentrations used in this study. Directionality was, however, observed in the flux of estrone sulfate (EsS). Adding probenecid, penicillin G or nonradiolabeled EsS to the apical donor compartment significantly increased the apical‐to‐basolateral flux of EsS across the BME‐UV monolayer. These results suggest that BME‐UV cells express an organic anion transport system, making it a potentially useful model to study the role of this transport system in the mammary epithelial barrier.
Onshore record of the subduction of a crustal salient: example of the NW Borneo Wedge
Authors: Sapin François , Pubellier Manuel , Lahfid Abdeltif , Janots Dominik , Aubourg Charles , Ringenbach Jean‐Claude 
Published: May 2011

Terra Nova, 23, 232–240, 2011AbstractSubduction of oceanic highs has been described in offshore accretionary wedges thanks to bathymetric and seismic data, and simulated in sand‐box models. Where documented, it is limited to oceanic subduction of small culminations (seamounts). However, tracing this phenomenon in ancient and/or uplifted belts remains a challenge. Based on structural, geomorphological and thermal analysis, we describe a subducted horst in the NW Borneo Wedge. This is evidenced by the occurrence of several geomorphological anomalies: a strong bend of the structural trend of the Rajang–Crocker Belt and an area with ′hummocky′ texture representing dismantled packages of sediments. Some specific structural features (large back and out‐of‐sequence thrusts in the internal zones, complex folds rooted on shear structures in the accretionary wedge) also occur in this central part of the NW Borneo Wedge. The central area of the related deformation zone records several thermal anomalies.Terra Nova, 00, 1–9, 2011
Are the oroclines of the Variscan belt related to late Variscan strike‐slip tectonics?
Authors: Martínez Catalán José R. 
Published: May 2011

Terra Nova, 23, 241–247, 2011AbstractEarly Variscan structures and magnetic anomalies delineate four oroclines along the Variscan belt. The arcs bend the lithostratigraphic zones of the autochthon and the allochthonous terranes, which include ophiolites of the Rheic suture and units of Gondwanan derivation subducted on both sides of the Rheic Ocean. A transcurrent model dominated by right‐lateral motion explains the genesis and evolution of the oroclines and a simple plate‐tectonics scenario links late Variscan dextral strike‐slip movements in the Variscides to a broad intracontinental shear zone, kinematically equivalent to a transform fault.Terra Nova, 00, 1–7, 2011
The key enzyme of sulfate assimilation, adenosine 5′‐phosphosulfate reductase, is regulated by HY5 in Arabidopsis
Authors: Lee Bok‐Rye , Koprivova Anna , Kopriva Stanislav 
Published: May 2011

SummaryPlant sulfate assimilation is regulated by demand for reduced sulfur, as is its key enzyme, adenosine 5′‐phosphosulfate reductase (APR). In a genetic screen for mutants lacking this regulation, we identified the bZIP transcription factor LONG HYPOCOTYL 5 (HY5) as a necessary component of the regulatory circuit. Regulation of APR activity by the inhibitor of glutathione synthesis, buthionine sulfoximine, or by the precursor of cysteine, O‐acetylserine, was disrupted in the hy5 mutant. When dark‐adapted plants were re‐illuminated, the rapid induction of APR1 and APR2 mRNA levels was attenuated in hy5 seedlings, but APR3 regulation was not affected. Chromatin immunoprecipitation revealed that HY5 binds directly to the APR1 and APR2 promoters but not to the APR3 promoter. Accordingly, the regulation of APR1 and APR2 by O‐acetylserine was disturbed in hy5 roots. HY5 is also important for the coordination of nitrogen and sulfur assimilation, as, unlike the wild‐type, hy5 mutants do not undergo a reduction in sulfate uptake and APR activity during nitrogen starvation. Altogether, these data show that HY5 plays an important role in regulation of APR gene expression and plant sulfate assimilation.
Dual functions of the nucleus‐encoded factor TDA1 in trapping and translation activation of atpA transcripts in Chlamydomonas reinhardtii chloroplasts
Authors: Eberhard Stephan , Loiselay Christelle , Drapier Dominique , Bujaldon Sandrine , Girard‐Bascou Jacqueline , Kuras Richard , Choquet Yves , Wollman Francis‐André 
Published: May 2011

SummaryAfter endosymbiosis, organelles lost most of their initial genome. Moreover, expression of the few remaining genes became tightly controlled by the nucleus through trans‐acting protein factors that are required for post‐transcriptional expression (maturation/stability or translation) of a single (or a few) specific organelle target mRNA(s). Here, we characterize the nucleus‐encoded TDA1 factor, which is specifically required for translation of the chloroplast atpA transcript that encodes subunit α of ATP synthase in Chlamydomonas reinhardtii. The sequence of TDA1 contains eight copies of a degenerate 38‐residue motif, that we named octotrico peptide repeat (OPR), which has been previously described in a few other trans‐acting factors targeted to the C. reinhardtii chloroplast. Interestingly, a proportion of the untranslated atpA transcripts are sequestered into high‐density, non‐polysomic, ribonucleoprotein complexes. Our results suggest that TDA1 has a dual function: (i) trapping a subset of untranslated atpA transcripts into non‐polysomic complexes, and (ii) translational activation of these transcripts. We discuss these results in light of our previous observation that only a proportion of atpA transcripts are translated at any given time in the chloroplast of C. reinhardtii.
BON1 interacts with the protein kinases BIR1 and BAK1 in modulation of temperature‐dependent plant growth and cell death in Arabidopsis
Authors: Wang Zheng , Meng Pei , Zhang Xiaoyan , Ren Dongtao , Yang Shuhua 
Published: May 2011

SummaryThe Arabidopsis copine gene BON1 encodes a calcium‐dependent phospholipid‐binding protein involved in plant growth homeostasis and disease resistance. However, the biochemical and molecular mechanisms by which BON1 modulates plant growth and defense responses are not well understood. Here, we show that BON1 interacts physically with the leucine‐rich‐repeat receptor‐like kinases BIR1 (BAK1‐interacting receptor‐like kinase 1) and pathogen‐associated molecular pattern (PAMP) receptor regulator BAK1 in vitro and in vivo. Additionally, bon1 and bir1 mutants exhibit synergistic interaction. While a bir1 null mutant has similar growth and cell‐death defects compared with bon1, a bir1 bon1 double mutant displays more severe phenotypes than does the single mutants. The bon1‐1 and bir1‐1 phenotypes are partially suppressed by overexpression of BIR1 and BON1, respectively. Furthermore, the bir1 phenotype is attenuated by a loss‐of‐function mutation in the resistance (R) gene SNC1 (Suppressor of npr1‐1, constitutive 1), which mediates defense responses in bon1. Intriguingly, BON1 and BIR1 can be phosphorylated by BAK1 in vitro. Our findings suggest that BIR1 functions as a negative regulator of plant resistance and that BON1 and BIR1 might modulate both PAMP‐ and R protein‐triggered immune responses.
Integrating long‐day flowering signals: a LEAFY binding site is essential for proper photoperiodic activation of APETALA1
Authors: Benlloch Reyes , Kim Min Chul , Sayou Camille , Thévenon Emmanuel , Parcy Francois , Nilsson Ove 
Published: May 2011

SummaryThe transition to flowering in Arabidopsis is characterized by the sharp and localized upregulation of APETALA1 (AP1) transcription in the newly formed floral primordia. Both the flower meristem‐identity gene LEAFY (LFY) and the photoperiod pathway involving the FLOWERING LOCUS T (FT) and FD genes contribute to this upregulation. These pathways have been proposed to act independently but their respective contributions and mode of interaction have remained elusive. To address these questions, we studied the AP1 regulatory region. Combining in vitro and in vivo approaches, we identified which of the three putative LFY binding sites present in the AP1 promoter is essential for its activation by LFY. Interestingly, we found that this site is also important for the correct photoperiodic‐dependent upregulation of AP1. In contrast, a previously proposed putative FD‐binding site appears dispensable and unable to bind FD and we found no evidence for FD binding to other sites in the AP1 promoter, suggesting that the FT/FD‐dependent activation of AP1 might be indirect. Altogether, our data give new insight into the interaction between the FT and LFY pathways in the upregulation of AP1 transcription under long‐day conditions.
Revealing impaired pathways in the an11 mutant by high‐throughput characterization of Petunia axillaris and Petunia inflata transcriptomes
Authors: Zenoni Sara , D’Agostino Nunzio , Tornielli Giovanni B. , Quattrocchio Francesca , Chiusano Maria L. , Koes Ronald , Zethof Jan , Guzzo Flavia , Delledonne Massimo , Frusciante Luigi , Gerats Tom , Pezzotti Mario 
Published: May 2011

SummaryPetunia is an excellent model system, especially for genetic, physiological and molecular studies. Thus far, however, genome‐wide expression analysis has been applied rarely because of the lack of sequence information. We applied next‐generation sequencing to generate, through de novo read assembly, a large catalogue of transcripts for Petunia axillaris and Petunia inflata. On the basis of both transcriptomes, comprehensive microarray chips for gene expression analysis were established and used for the analysis of global‐ and organ‐specific gene expression in Petunia axillaris and Petunia inflata and to explore the molecular basis of the seed coat defects in a Petunia hybrida mutant, anthocyanin 11 (an11), lacking a WD40‐repeat (WDR) transcription regulator. Among the transcripts differentially expressed in an11 seeds compared with wild type, many expected targets of AN11 were found but also several interesting new candidates that might play a role in morphogenesis of the seed coat. Our results validate the combination of next‐generation sequencing with microarray analyses strategies to identify the transcriptome of two petunia species without previous knowledge of their genome, and to develop comprehensive chips as useful tools for the analysis of gene expression in P. axillaris, P. inflata and P. hybrida.
Multicentre prospective study on dengue classification in four South‐east Asian and three Latin American countries
Authors: Alexander Neal , Balmaseda Angel , Coelho Ivo C. B. , Dimaano Efren , Hien Tran T. , Hung Nguyen T. , Jänisch Thomas , Kroeger Axel , Lum Lucy C. S. , Martinez Eric , Siqueira Joao B. , Thuy Tran T. , Villalobos Iris , Villegas Elci , Wills Bridget ,   on behalf of the European Union, World Health Organization (WHO‐TDR) supported DENCO Study Group
Published: May 2011

SummaryObjective  To evaluate the existing WHO dengue classification across all age groups and a wide geographical range and to develop a revised evidence‐based classification that would better reflect clinical severity.Methods  We followed suspected dengue cases daily in seven countries across South‐east Asia and Latin America and then categorised them into one of three intervention groups describing disease severity according to the overall level of medical and nursing support required. Using a pre‐defined analysis plan, we explored the clinical and laboratory profiles characteristic of these intervention categories and presented the most promising options for a revised classification scheme to an independent group of WHO dengue experts for consideration. Potential warning signs were also evaluated by comparing contemporaneous data of patients who progressed to severe disease with the data of those who did not.Results  A total of 2259 patients were recruited during 2006–2007 and 230 (13%) of the 1734 laboratory‐confirmed patients required major intervention. Applying the existing WHO system, 47/210 (22%) of patients with shock did not fulfil all the criteria for dengue haemorrhagic fever. However, no three‐tier revision adequately described the different severity groups either. Inclusion of readily discernible complications (shock/severe vascular leakage and/or severe bleeding and/or severe organ dysfunction) was necessary to devise a system that identified patients requiring major intervention with sufficient sensitivity and specificity to be practically useful. Only a small number of subjects (5%) progressed to severe disease while under observation; several warning signs were identified, but much larger studies are necessary to fully characterize features associated with disease progression.Conclusions  Based on these results, a revised classification system comprised of two entities, ‘Dengue’ and ‘Severe Dengue’, was proposed and has now been incorporated into the new WHO guidelines.
Monitoring drug effectiveness in kala‐azar in Bihar, India: cost and feasibility of periodic random surveys vs. a health service‐based reporting system
Authors: Malaviya P. , Singh R. P. , Singh S. P. , Hasker E. , Ostyn B. , Shankar R. , Boelaert M. , Sundar S. 
Published: May 2011

SummaryObjective  In 2009, a random survey was conducted in Muzaffarpur district to document the clinical outcomes of visceral leishmaniasis patients (VL) treated by the public health care system in 2008, to assess the effectiveness of miltefosine against VL. We analysed the operational feasibility and cost of such periodic random surveys as compared with health facility‐based routine monitoring.Methods  A random sample of 150 patients was drawn from registers kept at Primary Health Care centres. Patient records were examined, and the patients were located at their residence. Patients and physicians were interviewed with the help of two specifically designed questionnaires by a team of one supervisor, one physician and one field worker. Costs incurred during this survey were properly documented, and vehicle log books maintained for analysis.Results  Hundred and 39 (76.7%) of the patients could be located. Eleven patients were not traceable. Per patient, follow‐up cost was US$ 15.51 and on average 2.27 patients could be visited per team‐day. Human resource involvement constituted 75% of the total cost whereas involvement of physician costs 51% of the total cost.Conclusion  A random survey to document clinical outcomes is costly and labour intensive but gives probably the most accurate information on drug effectiveness. A health service‐based retrospective cohort reporting system modelled on the monitoring system developed by tuberculosis programmes could be a better alternative. Involvement of community health workers in such monitoring would offer the additional advantage of treatment supervision and support.
The effect of oral isotretinoin (13‐cis retinoic acid) on hearing systems in patients with acne vulgaris: a prospective study
Authors: Karabulut Hayriye , Karadag Ayse Serap , Acar Baran , Dagli Muharrem , Karabulut Ismail , Ozmen Erkan , Babademez Mehmet Ali , Karaşen Rıza Murat 
Published: May 2011

AbstractIsotretinoin is widely used in the treatment of extensive and nodulocystic acne. The objective of this prospective study was to investigate whether oral isotretinoin could affect the hearing system. Thirty‐eight patients with acne vulgaris (76 ears) who were diagnosed and treated at the Department of Dermatology were included in the current study. Study evaluation visits were performed at baseline and at Weeks 1, 2 and 3. Pure‐tone averages (PTAs) of air conduction thresholds at 250 Hz (PTA1); 500, 1000, and 2000 Hz (PTA2); 4000, 8000, and 10, 000 Hz (PTA3); and 12, 500, 16, 000, 18, 000 and 20, 000 Hz (PTA4) for each ear were calculated separately. Assessment of the efficacy was based on the audiometric findings. Compared with pre‐treatment evaluation, the PTAs of patients were found to be significantly different at the first week for PTA2 (P = 0.033) and PTA3 (P = 0.001), at the second week for PTA1 (P = 0.036), and at the third week for PTA4 (P = 0.002). Our results suggest that the oral isotretinoin (13‐cis retinoic acid), which is a derivative of retinol (vitamin A), improved the hearing level of the patients in all audiometric frequencies in a short‐period follow‐up.
Imagined futures: how experiential knowledge of disability affects parents’ decision making about fetal abnormality
Authors: France Emma F. , Locock Louise , Hunt Kate , Ziebland Sue , Field Kate , Wyke Sally 
Published: May 2011

AbstractBackground  Knowledge of disability is considered key information to enable informed antenatal screening decisions by expectant parents. However, little is known about the role of experiential knowledge of disability in decisions to terminate or continue with a pregnancy diagnosed with a fetal abnormality.Objective  To explore the role that expectant parents’ experiential knowledge of disabilities and conditions can play in real‐life decisions to continue or end a pregnancy with a fetal abnormality.Design  Secondary analysis of qualitative narrative interview data informed by contextual systems framework.Setting  Participants were recruited throughout the United Kingdom and interviewed between 2004 and 2006.Participants  Twenty‐four women and four of their male partners who had direct or indirect experience of disability or illness and who had proceeded with or ended a pregnancy diagnosed with a fetal abnormality.Findings  Most respondents recounted using their experiential knowledge of disability, whether of their unborn baby’s condition or of a different condition, to try to imagine the future for their unborn child, themselves and their family when making their decision. Some, who were considering continuing their pregnancy and had little or no experience of their unborn baby’s specific disability, sought out others’ experiences of the condition following antenatal diagnosis.The nature of a parent’s experiential knowledge did not predict whether they continued with or terminated their pregnancy.Discussion  Prospective parents may find it helpful to discuss their existing knowledge of their unborn baby’s condition with health professionals who are aware of the influence this might have on parents’ decisions.