bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Randomized Clinical Evaluation of a One- and Two-step Self-etch Adhesive Over 24 Months
Authors: Loguercio A. D. , Mânica D. , Ferneda F. , Zander-Grande C. , Amaral R. , Stanislawczuk R. , Carvalho R. M. , Manso A. , Reis A. 
Published: May 2010

Clinical Relevance
The application of an extra hydrophobic bond layer over the self-etch adhesive system improved clinical performance over a 24-month period, mainly in terms of retention rate.
Effects of Blood Contamination on Microtensile Bond Strength to Dentin of Three Self-etch Adhesives
Authors: Chang S. W. , Cho B. H. , Lim R. Y. , Kyung S. H. , Park D. S. , Oh T. S. , Yoo H. M. 
Published: May 2010

Clinical Relevance
When blood contamination occurs during dentin bonding with self-etch adhesives, blood contamination cannot be adequately removed by water rinsing alone.
Influence of Curing Mode and Time on Degree of Conversion of One Conventional and Two Self-adhesive Resin Cements
Authors: Aguiar T. R. , Francescantonio M. D. , Arrais C. A. G. , Ambrosano G. M. B. , Davanzo C. , Giannini M. 
Published: May 2010

Clinical Relevance
Within the limited experimental conditions, self-adhesive cements provided a higher degree of conversion values when light-activated. In addition, the results showed that, after 15 minutes, the degree of conversion values increased for all materials in both activation modes.
Histology of Irreversible Pulpitis Premolars Treated with Mineral Trioxide Aggregate Pulpotomy
Authors: Chueh L-H. , Chiang C-P. 
Published: May 2010

Clinical RelevanceWhen caries and bacterial contamination can be mostly eliminated from the dentin-pulp complex, the inflamed pulp of a human permanent tooth with irreversible pulpitis may have a chance to return to a healthy and functional status after mineral trioxide aggregate pulpotomy.
Alternative Technique for Class V Resin Composite Restorations with Minimum Finishing/Polishing Procedures
Authors: Perez C. R. 
Published: May 2010

Clinical Relevance
Class V restorations are a common challenge to clinicians. These restorations involve problems, including isolation, adhesion, insertion technique, finishing and polishing. In order to solve these problems, several techniques have been developed. This article describes a new technique for the restoration of Class V cavities, one that eliminates rubber dam isolation and avoids any contamination. If used properly, this technique also provides good cervical contour, reducing or eliminating finishing and polishing procedures.
Comparative In Vivo Study on the Desensitizing Efficacy of Dentin Desensitizers and One-bottle Self-etching Adhesives
Authors: Yu X. , Liang B. , Jin X. , Fu B. , Hannig M. 
Published: May 2010

Clinical Relevance
Dentin desensitizers and one-bottle self-etching adhesives can significantly reduce dentin hypersensitivity. Clinical desensitizing effectiveness depends on the individual dentin desensitizers/adhesives used.
Assessment of laparoscopic skills before and after simulation training with a canine abdominal model
Authors: Fransson Boel A. , Ragle Claude A. 
Published: May 2010

Abstract
Objective—To determine whether scores for basic laparoscopic skills were significantly associated with extent of laparoscopic experience and compare basic laparoscopic skill scores obtained before and after 2 laparoscopic training sessions incorporating a canine abdominal model.
Design—Evaluation study.
Sample Population—8 experienced and 25 novice individuals.
Procedures—Novice participants were randomly assigned to control (n = 10) and training (15) groups. Individuals in the experienced and novice training groups were required to undergo 2 training sessions with a canine abdominal model. Basic laparoscopic skills were assessed twice on the basis of 3 tasks included in the McGill Inanimate Simulator for Training and Evaluation of Laparoscopic Skills (MISTELS).
Results—For the novice training group, laparoscopic skills scores were significantly higher after training than before, but for individuals in the novice control group, scores did not differ significantly between the first and second assessments. The increase in score for the novice training group was significantly higher than increases for the experienced group and for the novice control group, but the increase in score for the experienced group was not significantly different from the increase in score for the novice control group.
Conclusions and Clinical Relevance—Results suggested that basic laparoscopic skills scores obtained with the MISTELS were associated with extent of laparoscopic experience and that training with a canine abdominal model could increase skills scores for individuals without previous laparoscopic experience.
Use of stored equine colostrum for the treatment of foals perceived to be at risk for failure of transfer of passive immunity
Authors: Nath Laura C. , Anderson Garry A. , Savage Catherine J. , McKinnon Angus O. 
Published: May 2010

Abstract
Objective—To assess the use of stored equine colostrum for the treatment of foals perceived to be at risk for failure of transfer of passive immunity (FTPI).
Design—Cohort study.
Animals—232 Thoroughbred foals and 191 Thoroughbred mares (41 mares gave birth to 1 foal on 2 occasions).
Procedures—Postpartum, presuckle colostrum samples were collected from mares; samples with a colostral refractive index (cRI) ≥ 23% were frozen (−20°C [–4°F]) and stored for ≥ 7 days but < 2 years. Foals of dams that produced colostrum with a cRI value < 20% were treated with ≥ 300 mL of stored colostrum that was thawed and administered via nasogastric tube on 1 to 4 occasions within 6 hours after parturition. Serum samples were obtained from colostrum-treated and nontreated foals 24 hours after treatment or suckling, respectively, for determination of serum IgG (sIgG) concentration.
Results—8 foals and their respective dams were excluded from the analyses. For the remaining 30 treated and 194 nontreated foals, mean ± SD sIgG concentration was 1,597 ± 574 mg/dL. Thirteen (5.8%) foals had sIgG concentrations < 800 mg/dL, of which 1 (0.4%) had an sIgG concentration < 400 mg/dL. Nine of these foals had suckled mares producing colostrum with a cRI value ≥ 20%, and 2 foals had been treated with stored colostrum.
Conclusions and Clinical Relevance—Treatment with stored colostrum appeared to be effective for prevention of FTPI in at-risk foals. However, foals were still at risk for FTPI despite suckling of or treatment with colostrum with adequate cRI values.
Comparison of the effects of intravenous administration of isotonic and hypertonic sodium bicarbonate solutions on venous acid-base status in dehydrated calves with strong ion acidosis
Authors: Coskun Alparslan , Sen Ismail , Guzelbektes Hasan , Ok Mahmut , Turgut Kursat , Canikli Sebnem 
Published: May 2010

Abstract
Objective—To compare the effects of IV administration of isotonic (1.3%) and hypertonic (8.4%) sodium bicarbonate (NaHCO3) solutions on acid-base status in dehydrated calves with strong ion (metabolic) acidosis.
Design—Randomized controlled clinical trial.
Animals—50 calves with diarrhea and severe dehydration.
Procedures—Calves were randomly assigned to receive isotonic NaHCO3 solution (65 mL/kg [29.5 mL/lb], IV) over 3 hours (n = 30) or hypertonic NaHCO3 solution (10 mL/kg [4.5 mL/lb], IV) over 20 minutes (20). Blood samples were collected at 0 hours (immediately prior to solution administration) and at 0.5, 1, 2, and 4 hours after administration began. Samples were submitted for blood gas analysis, serum biochemical analysis, and determination of blood Na+, K+, and Cl− concentrations and percentage change in plasma volume.
Results—Calves that received isotonic NaHCO3 solution had an increase in venous blood pH, HCO3 concentration, and base excess; a small, transient increase in Po2; and no change in Pco2 within 4 hours after administration began. Calves that received hypertonic NaHCO3 solution had an immediate increase in venous blood pH, HCO3 concentration, and base excess; a small, transient increase Pco2; and no change in Po2 within 0.5 hours after treatment began. Plasma volume increased to a greater extent following administration of isotonic solution than after administration of hypertonic solution.
Conclusions and Clinical Relevance—IV administration of 8.4% NaHCO3 solution in small volumes provided fast and effective improvement of severe acid-base abnormalities in calves with severe strong ion acidosis but did not improve hydration status as well as administration of a larger volume of isotonic NaHCO3 solution.
Effects of anesthesia and surgery on serial blood gas values and lactate concentrations in yellow perch (Perca flavescens), walleye pike (Sander vitreus), and koi (Cyprinus carpio)
Authors: Hanley Christopher S. , Clyde Victoria L. , Wallace Roberta S. , Paul-Murphy Joanne , Patterson Tamatha A. , Keuler Nicholas S. , Sladky Kurt K. 
Published: May 2010

Abstract
Objective—To evaluate serial blood gas values and lactate concentrations in 3 fish species undergoing surgery and to compare blood lactate concentrations between fish that survived and those that died during the short-term postoperative period.
Design—Prospective cohort study.
Animals—10 yellow perch, 5 walleye pike, and 8 koi.
Procedures—Blood samples were collected from each fish at 3 time points: before anesthesia, during anesthesia, and immediately after surgery. Blood gas values and blood lactate concentrations were measured. Fish were monitored for 2 weeks postoperatively.
Results—All walleye and koi survived, but 2 perch died. Blood pH significantly decreased in perch from before to during anesthesia, but increased back to preanesthesia baseline values after surgery. Blood Pco2 decreased significantly in perch from before anesthesia to immediately after surgery, and also from during anesthesia to immediately after surgery, whereas blood Pco2 decreased significantly in koi from before to during anesthesia. Blood Po2 increased significantly in both perch and koi from before to during anesthesia, and also in koi from before anesthesia to immediately after surgery. For all 3 species, blood lactate concentrations increased significantly from before anesthesia to immediately after surgery. Blood lactate concentration (mean ± SD) immediately after surgery for the 8 surviving perch was 6.06 ± 1.47 mmol/L, which was significantly lower than blood lactate concentrations in the 2 nonsurviving perch (10.58 and 10.72 mmol/L).
Conclusions and Clinical Relevance—High blood lactate concentrations following surgery in fish may be predictive of a poor short-term postoperative survival rate.
Correlation between cognitive impairment and cerebral hemodynamic disturbances on perfusion magnetic resonance imaging in European adults with moyamoya disease
Authors: Calviere Lionel , Catalaa Isabelle , Marlats Fabienne , Viguier Alain , Bonneville Fabrice , Cognard Christophe , Larrue Vincent 
Published: May 2010

Object
Although cognitive impairment has been reported in adults with moyamoya disease (MMD), its relationship with cerebral hemodynamic disturbances has not been investigated. The aims of the present study were to confirm the presence of dysexecutive cognitive syndrome (DCS) in adults with MMD and to explore the relationship of DCS with frontal lobe perfusion as measured by perfusion MR imaging.

Methods
Cerebral blood volume (CBV) ratio and mean transit time delay were measured in frontal and temporoparietal regions using the cerebellum as a reference region in 10 European adults with MMD. In addition, the authors calculated the cerebrovascular reserve (CVR) using the CBV ratio and the acetazolamide challenge. All patients underwent a standardized neuropsychological assessment test battery. The authors defined DCS as an impairment shown on 3 tests or more of executive function.

Results
The authors found DCS in 6 patients. The frontal CVR was lower in patients with DCS than in patients without DCS (mean ± SD: −13.5 ± 13.2% and 20.3 ± 21.3%; p = 0.019, Mann-Whitney U-test). Other parameters of frontal perfusion and temporoparietal CVR were not correlated with DCS.

Conclusions
The authors' findings suggest that DCS is common in European adults with MMD and may be related to frontal perfusion impairment.
Temozolomide-induced inhibition of pituitary adenoma cells
Authors: Sheehan Jason , Rainey Jessica , Nguyen James , Grimsdale Ruthie , Han Shaojie 
Published: May 2010

Object
Aggressive pituitary adenomas frequently require multimodality treatment including pituitary-suppressive medications, microsurgery, and radiation therapy or radiosurgery. The effectiveness of temozolomide in terms of growth suppression and decreased hormonal production is evaluated.

Methods
Three pituitary adenoma cell lines—MMQ, GH3, and AtT20—were used. A dose escalation of temozolomide was performed for each cell line, and inhibition of cell proliferation was assessed using an MTT assay. Concentrations of temozolomide that produced statistically significant inhibition of cell proliferation for each cell type were identified. Extent of apoptosis for each selected temozolomide concentration was studied using TUNEL staining. The effect of temozolomide on prolactin secretion in MMQ and GH3 cells was also measured via ELISA.

Results
Significant inhibition of cell proliferation was noted for MMQ and GH3 cells at a concentration of 250 μM temozolomide. The AtT20 cells demonstrated statistically significant cell inhibition at a concentration of only 50 μM temozolomide (p < 0.05). Apoptosis significantly increased in all cell lines in as little as 24 hours of incubation at the respective temozolomide concentrations (p < 0.05). Prolactin secretion in the prolactin secreting MMQ and GH3 cell lines was inhibited by 250 μM temozolomide.

Conclusions
Temozolomide inhibits cell proliferation and induces apoptotic cell death in aggressive pituitary adenoma cells. A reduction in hormonal secretion in prolactinoma cells was also afforded by temozolomide. Temozolomide may prove useful in the multimodality management of aggressive pituitary adenomas.
Effectiveness of Leflunomide in Patients with Juvenile Idiopathic Arthritis in Clinical Practice
Authors: FOELDVARI IVAN , WIERK ANGELA 
Published: May 2010

Objective.To evaluate the effectiveness of leflunomide in children with juvenile idiopathic arthritis (JIA) as used in actual clinical practice.Methods.We conducted a retrospective review of medical records of patients with JIA who initiated leflunomide treatment between April 2001 and October 2006. Data derived from these charts included patient baseline characteristics, reason for starting leflunomide, adverse events, joint outcomes, Childhood Health Assessment Questionnaire (CHAQ) scores, visual analog scale pain and well-being scores, and current treatment status.Results.Fifty-eight patients (33 female, 25 male) were included in this study. Forty-eight patients were switched from methotrexate (MTX) to leflunomide, primarily because of MTX-related adverse events, and leflunomide was added to ongoing MTX in 10 patients. The mean duration of leflunomide therapy was 1.45 years. The mean swollen joint count decreased from 1.40 at treatment initiation to 0.60 at last followup, while the mean tender joint count decreased from 1.83 to 0.29. Improvements were also observed in CHAQ, pain, and well-being scores. At last followup, 44.8% of patients were continuing leflunomide therapy, 29.3% had discontinued because of remission, and the rest had discontinued treatment because of side effects (22.4%) or other reasons (3.4%).Conclusion.Leflunomide treatment, as employed in actual clinical practice, was well tolerated and resulted in substantial improvements in joint and functional status outcomes in children with JIA. Approximately 30% of the patients attained remission during leflunomide therapy. Leflunomide is thus a safe and effective alternative for patients with JIA who cannot tolerate or do not respond to MTX monotherapy.
Tumor Necrosis Factor-α Blockers in SAPHO Syndrome: Table 1.
Authors: BEN ABDELGHANI KAOUTHER , DRAN DELPHINE GERARD , GOTTENBERG JACQUES-ERIC , MOREL JACQUES , SIBILIA JEAN , COMBE BERNARD 
Published: May 2010

Objective.To analyze the clinical efficacy of anti-tumor necrosis factor-α (TNF-α) therapy in treatment of synovitis, acne, pustulosis, hyperostosis, osteitis (SAPHO) syndrome, we describe cases of refractory SAPHO syndrome and review cases treated with anti-TNF-α reported in the literature.Methods.We describe 6 cases of patients with SAPHO syndrome treated with anti-TNF-α between 2004 and 2008. Therapeutic response was evaluated according to improvement in pain score, amelioration of disease activity, and improvement in function. The efficacy of treatment was considered to be reduced need for analgesics and/or antiinflammatory therapy.Results.In our series, 4 patients received infliximab, 1 etanercept, and 1 adalimumab. These treatments brought clinical response in 4 patients (66.6%): response was sustained with infliximab in 1 case for 7 months; with adalimumab in another case for 22 months; and with etanercept in 2 cases for 1 and 42 months, respectively. In contrast, 2 other patients showed no response to infliximab. Improvement was initially temporary after infusions 1 and 2, then pain recurred at Week 14. Skin lesions were healed in 3 of 4 cases, but recurred or worsened in 2 cases, after infusion 2 of infliximab. Treatment was generally well tolerated. Paradoxical psoriasis was noted in 2 cases and urticaria in 1.Conclusion.Given our results and those from the literature, TNF-α blockers should be considered in the therapeutic strategy of refractory cases of SAPHO syndrome, despite their effect seeming less impressive than in other spondyloarthropathies.
The Course of Symptoms for Whiplash-Associated Disorders in Sweden: 6-Month Followup Study
Authors: CRUTEBO SARA , NILSSON CHARLOTT , SKILLGATE EVA , HOLM LENA W. 
Published: May 2010

Objective.To describe symptom patterns and the course for recovery in persons with whiplash-associated disorders (WAD) over 6 months after a car collision, and to investigate associated gender differences.Methods.The study population was based on insurance claimants, 18–74 years of age, who reported WAD after a collision, between January 2004 and January 2005. At baseline and again 6 months later they were asked to complete a questionnaire that included questions about presence and severity of pain and other possible WAD symptoms. It also included measurements of posttraumatic stress as well as anxiety and depression.Results.A total of 1105 persons were studied. The most common symptoms at baseline after neck pain were reduced cervical range of motion (in 83.9% of men, 82.2% of women), headache (61.0% and 69.3%, respectively), and low back pain (35.9% and 36.1%). Some symptoms were already transient at baseline and symptoms such as neck pain, reduced cervical range of motion, headache, and low back pain decreased further over the 6 months. Baseline prevalence of depression was around 5% in both women and men, whereas posttraumatic stress and anxiety were more common in women (19.7% and 11.7%, respectively) compared to men (13.2% and 8.6%). The majority of all reported associated symptoms were mild at both baseline and followup.Conclusion.Our findings support that the symptom pattern of WAD and the prevalence for many of the symptoms decreased over a 6-month period.
Role of BANK1 Gene Polymorphisms in Biopsy-proven Giant Cell Arteritis
Authors: TORRES ORLANDO , PALOMINO-MORALES ROGELIO , CASTAÑEDA SANTOS , VAZQUEZ-RODRIGUEZ TOMAS R. , MORADO INMACULADA C. , MIRANDA-FILLOY JOSE A. , AMIGO-DIAZ ENCARNACION , VICENTE ESTHER F. , ORTEGO-CENTENO NORBERTO , FERNANDEZ-GUTIERREZ BENJAMIN , MARTIN JAVIER , GONZALEZ-GAY MIGUEL A. 
Published: May 2010

Objective.Giant cell arteritis (GCA) is a complex polygenic disease in which more than 1 genetic locus is likely to contribute to disease susceptibility and clinical expression. BANK, an adaptor molecule, has been suggested to participate in the B cell antigen receptors-mediated calcium homeostasis. We assessed for the first time the implication of BANK1 functional variants in susceptibility to GCA.Methods.Two hundred twenty-two patients with biopsy-proven GCA and 534 matched controls were assessed. DNA from patients and controls was obtained from peripheral blood. Samples were genotyped for 3 putative functional BANK1 gene polymorphisms (rs17266594 T/C, rs10516487 G/A, rs3733197 G/A) using a TaqMan allele discrimination assay.Results.No significant differences were observed in genotype distribution between patients with biopsy-proven GCA and controls for these 3 gene polymorphisms. A trend for a decreased risk of having GCA was observed in individuals carrying the BANK1 rs3733197 GG genotype (patients with GCA 43.9% compared to 51.6% in controls; p = 0.06, OR 0.73, 95% CI 0.53–1.02). The frequency of BANK1 rs3733197 allele G was marginally decreased in patients with biopsy-proven GCA compared to controls (p = 0.09, OR 0.82, 95% CI 0.64–1.04). Haplotype analysis of 3-single-nucleotide polymorphisms found no statistically significant differences between patients with GCA and controls. No significant differences for the BANK1 gene polymorphisms were found when patients were stratified according to specific clinical features of the disease.Conclusion.Our results do not support a major implication of the BANK1 locus in susceptibility to GCA.
Invariant NKT Cell Development Requires a Full Complement of Functional CD3 ζ Immunoreceptor Tyrosine-Based Activation Motifs
Authors: Becker Amy M. , Blevins Jon S. , Tomson Farol L. , Eitson Jennifer L. , Medeiros Jennifer J. , Yarovinsky Felix , Norgard Michael V. , van Oers Nicolai S. C. 
Published: May 2010

Abstract
Invariant NKT (iNKT) cells regulate early immune responses to infections, in part because of their rapid release of IFN-γ and IL-4. iNKT cells are proposed to reduce the severity of Lyme disease following Borrelia burgdorferi infection. Unlike conventional T cells, iNKT cells express an invariant αβ TCR that recognizes lipids bound to the MHC class I-like molecule, CD1d. Furthermore, these cells are positively selected following TCR interactions with glycolipid/CD1d complexes expressed on CD4+CD8+ thymocytes. Whereas conventional T cell development can proceed with as few as 4/10 CD3 immunoreceptor tyrosine-based activation motifs (ITAMs), little is known about the ITAM requirements for iNKT cell selection and expansion. We analyzed iNKT cell development in CD3 ζ transgenic lines with various tyrosine-to-phenylalanine substitutions (YF) that eliminated the functions of the first (YF1,2), third (YF5,6), or all three (YF1–6) CD3 ζ ITAMs. iNKT cell numbers were significantly reduced in the thymus, spleen, and liver of all YF mice compared with wild type mice. The reduced numbers of iNKT cells resulted from significant reductions in the expression of the early growth response 2 and promyelocytic leukemia zinc finger transcription factors. In the mice with few to no iNKT cells, there was no difference in the severity of Lyme arthritis compared with wild type controls, following infections with the spirochete B. burgdorferi. These findings indicate that a full complement of functional CD3 ζ ITAMs is required for effective iNKT cell development.