bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive flow cytometry phenotype in acute leukemia at diagnosis and at relapse
Authors: LI XIN , DU WEN , LIU WEI , LI XIAOQING , LI HONGRUI , HUANG SHI‐ANG 
Published: March 2010

Li X, Du W, Liu W, Li X, Li H, Huang S‐A. Comprehensive flow cytometry phenotype in acute leukemia at diagnostic and at relapse. APMIS 2010; 118: 353–59.Multiparameter flow cytometry (MFC) plays a vital role in the detection of minimal residual disease (MRD) and diagnosis of relapse in acute leukemia. However, application of a limited panel of antibodies in MFC leads to high rates of false‐negative and false‐positive results. Thirteen patients with acute lymphoblastic leukemia (ALL) and 12 patients with acute myeloid leukemia (AML) were immunophenotyped by MFC at diagnosis and at relapse using a comprehensive panel of monoclonal antibodies (McAbs) to 27 antigens and CD45/SSC gating. In 23 of 25 patients (92.3%), changes in at least one of progenitor‐associated, myeloid and lymphoid antigens between diagnosis and relapse were observed. Antigen changes were observed in 92 of 239 antigens (38.5%) expressed in 25 patients, in 49 of 117 antigens (41.9%) expressed in 13 ALL patients, and in 43 of 122 antigens (35.2%) expressed in 12 AML patients. Phenotypic changes were characterized by the expression of cross‐lineage antigens. The intralineage change was observed in the majority of patients. However, myeloid lineage shift was identified by MFC in two patients with T‐ALL. Multiple panels of three or more McAbs are likely to be required in the monitoring of MRD and diagnosis of relapse in acute leukemia by MFC.
Impact of defect configuration on the clinical outcome following surgical regenerative therapy of peri‐implantitis
Authors: Schwarz Frank , Sahm Narja , Schwarz Katrin , Becker Jürgen 
Published: March 2010

Schwarz F, Sahm N, Schwarz K, Becker J. Impact of defect configuration on the clinical outcome following surgical regenerative therapy of peri‐implantitis. J Clin Periodontol 2010; 37: 449–455. doi: 10.1111/j.1600‐051X.2010.01540.x.AbstractObjectives: The present study aimed at investigating the impact of defect configuration on the clinical outcome of surgical regenerative therapy of peri‐implantitis lesions using a natural bone mineral in combination with a collagen membrane (NBM+CM).Materials and Methods: Twenty‐seven patients (n=27 defects) exhibited three different types of peri‐implantitis lesions including either Class Ib (buccal dehiscence+semicircumferential), Class Ic (buccal dehiscence+circumferential), or Class Ie (circumferential) intra‐bony defects (n=9 defects per group). All defects were treated with access flap surgery and the application of NBM+CM.Results: At 6 and 12 months, Class Ie defects tended to reveal higher changes in the mean probing depth (PD) and clinical attachment level (CAL) values when compared with Class Ib and Class Ic groups. However, significant differences were only observed at 6 months (PD: 2.9 ± 0.3 versus 1.4 ± 0.5 versus 1.3 ± 0.7 mm; CAL: 2.5 ± 0.5 versus 0.9 ± 0.8 versus 0.9 ± 0.7 mm). Site‐level analysis has pointed to lowest PD and CAL changes at the midbuccal aspect of Class Ib and Class Ic groups.Conclusion: Defect configuration may have an impact on the clinical outcome following surgical regenerative therapy of peri‐implantitis lesions. While Class Ie defects seem to be promising in conjunction with NBM+CM, Class Ib and Class Ic may be considered as unfavourable.
Millennium development goals and oral health in cities in southern Brazil
Authors: Bueno Roberto Eduardo , Moysés Samuel Jorge , Moysés Simone Tetu 
Published: March 2010

Bueno RE, Moysés SJ, Moysés ST. Millennium development goals and oral health in cities in southern Brazil. Community Dent Oral Epidemiol 2010; 38: 197–205. © 2010 John Wiley & Sons A/SAbstract –  Objectives:  To investigate social determinants of oral health, analysing the occurrence of associations between millennium development goals (MDG) indicators and oral health (OH) indicators.Methods:  An ecological study was performed in two distinct phases. In Phase 1, MDG indicators and related covariates were obtained from the demographic census of the Brazilian Institute of Geography and Statistics, the Ministry of Health database and the 2000 Human Development Atlas, making up the whole set of independent variables. Principal component analysis was carried out for the independent variables showing the correlations among the variables comprising the main components, and generating a synthetic index allowing the performance of the cities to be known with regard to the MDG (MDG index). In Phase 2, the DMFT index (mean number of decay, missing or filled permanent teeth) and the CF index (prevalence of caries‐free individuals), in 12 years old were obtained from the epidemiological survey undertaken in 2002–2003, in 49 cities in southern Brazil, and were analysed in relation to the MDG index using Spearman’s correlation.Results:  A statistically significant correlation was found for the DMFT and CF indices, respectively, with: the MDG index (R2 = 0.49 and 0.48; P = 0.00); the socioeconomic status of the population (R2 = 0.12 and 0.12; P = 0.02); the socioenvironmental characteristics (R2 = 0.41 and 0.46; P = 0.00).Conclusions:  The MDG synthetic index of the cities analysed and the respective components relating to their socioeconomic and socioenvironmental status demonstrated a positive correlation with OH indicators. As such, intersectoral public policies based on population strategies that act on social determinants of general and oral health need to be integrated so as to impact on the MDG and OH outcomes.
Functional skin adaptation in infancy – almost complete but not fully competent
Authors: Fluhr Joachim W. , Darlenski Razvigor , Taieb Alain , Hachem Jean‐Pierre , Baudouin Caroline , Msika Philippe , De Belilovsky Clarence , Berardesca Enzo 
Published: March 2010

Please cite this paper as: Functional skin adaptation in infancy – almost complete but not fully competent. Experimental Dermatology 2010; 19: 483–492.Abstract:  Early postnatal life is a period of active functional reorganization and cutaneous physiological adaptation to the extrauterine environment. Skin as the outermost organ of mammalians is endowed of multiple functions such as protection, secretion, absorption and thermoregulation. Birth stimulates the epidermal barrier maturation and the skin surface acidification especially in premature infants. In full‐term infants the developed stratum corneum accomplishes competent barrier function, in contrast to prematures. Complete barrier maturation in preterm infants is fulfilled by 2–4 weeks of the postnatal life. However, in preterms with 23–25 weeks gestational age this process takes longer. Versatile regulatory mechanisms, namely skin surface acidity, calcium ion gradient and nuclear hormone receptors/ligands are interrelated in the complex postnatal newborn adaptation. The skin of newborns is adjusting quickly to the challenging environmental conditions of the postpartum. However, certain functions, for example, microcirculation, continue to develop even beyond the neonatal period, that is, up to the age of 14–17 weeks. Different environmental factors (for instance, dry and cold climate, diapers and cosmetic care procedures) influence the postnatal development of skin functional parameters such as stratum corneum hydration and the permeability barrier especially in premature infants. The aim of this article is to summarize the current knowledge on skin physiology in newborn and infants with a practical approach and to discuss the possible clinical consequences. This review offers the readership a critical and practical overview of skin physiology in newborns and infants. It emphasizes possible new research fields in neonatal and infantile skin physiology.
Posttraumatic Symptoms, Marital Intimacy, Dyadic Adjustment, and Sexual Satisfaction among Ex-Prisoners of War
Authors: Zerach Gadi , Anat Ben-David , Solomon Zahava , Heruti Rafi 
Published: March 2010

ABSTRACT

Introduction
The aversive impact of combat and combat-induced posttraumatic stress disorder (PTSD) on marital intimacy and sexual satisfaction has been examined in several studies. Nevertheless, the toll of war captivity on marital intimacy in relation to dyadic adjustment and sexual satisfaction remains unknown. In particular, the mediating role of marital intimacy in the relationship between PTSD symptoms and dyadic adjustment and between PTSD symptoms and sexual satisfaction has not yet been systematically explored thus far.

Aims
This study aimed to examine the interrelationships of PTSD symptoms, dyadic adjustment, sexual satisfaction, and marital intimacy among ex-prisoners of war (ex-POWs).

Methods
A sample of Israeli veterans ex-POWs (ex-POWs: N=105) from the 1973 Yom Kippur War and a matched comparison group of veterans who participated in the same war but were not held captive (control: N=94) were compared in the study variables.

Main Outcome Measures
The PTSD inventory, dyadic adjustment scale, index of sexual satisfaction, and capacity for intimacy questionnaire.

Results
Findings revealed that ex-POWs reported higher levels of PTSD symptoms and lower levels of dyadic adjustment and sexual satisfaction than comparable controls. There were also differences between the groups in the pattern of relations between PTSD symptoms, dyadic adjustment, sexual satisfaction, and marital intimacy. Finally, for ex-POWs, marital intimacy partially mediated the relationships between PTSD symptoms and dyadic adjustment and sexual satisfaction outcome measures.

Conclusions
PTSD symptoms are implicated in marital problems of ex-POWs. A significant relationship was found between the traumatized ex-POW’s capacity for intimacy and both their sexual satisfaction and dyadic adjustment.
Lifetime Sexual Behavior of Psychiatric Inpatients
Authors: Gonzalez-Torres Miguel Angel , Salazar Miguel Angel , Inchausti Lucía , Ibañez Berta , Pastor Javier , Gonzalez Gixane , Carvajal María Josefa , Fernandez-Rivas Aranzazu , Madrazo Aranzazu , Ruiz Eduardo , Basterreche Edurne 
Published: March 2010

ABSTRACT

Introduction
Sexual life of psychiatric patients, including risk behaviors related to sexually transmitted diseases, remains a poorly studied area, especially in those with severe mental illnesses.

Aims
To assess some aspects of lifetime sexual behavior of psychiatric inpatients.

Methods
Patients consecutively admitted to a psychiatric unit in a general hospital were interviewed about partner-related lifetime sexual behavior.

Main Outcome Measures
A semi-structured interview developed by the authors was used to collect information concerning their general sexual experience throughout life, homosexual and heterosexual relations, and relations with partners who were intravenous drug users (IVDU), HIV carriers or suffering from AIDS, and with sex workers. In each of these areas, time elapsed since last sexual contact; number of partners in previous year, frequency of these relations and condom use were investigated.

Results
Five hundred forty-six patients (306 men and 240 women) were assessed, and 87.7% of them reported sexual relations at some point during their life. Of these, 90% reported heterosexual and 10% homosexual or bisexual sexual contacts. Further, 11.06% had had at least one partner who was an IVDU; 8.1% an HIV-positive partner, and 32.4% (50% of the men) had paid for sex. Overall 49.79% of the total sample reported never using condoms in their sexual relations, with similar percentages for those with HIV-positive (46%) and IVDU (47%) partners. Of those who paid for sex, 29% never used condoms.

Conclusions
Psychiatric patients admitted to a general hospital psychiatric unit have sexual experience close to the general population, with a higher percentage of homosexual contacts and lower rates of condom use, even in higher risk situations, such as men having sex with men, and partners who are HIV-positive or IVDUs. This information obliges clinicians to systematically explore the sexual behavior of psychiatric patients, evaluate risk behaviors, and adopt measures to promote safe sex practices in this population.
Post Milk Quota Experiences in Switzerland  Expériences post‐contingent laitier en Suisse  Nach der Milchquote: Erfahrungen aus der Schweiz
Authors: Mann Stefan , Gairing Miriam 
Published: March 2010

summaryPost Milk Quota Experiences in SwitzerlandSwitzerland can be considered a test bed for the EU’s withdrawal from the milk quota scheme. The Swiss government enacted an exit phase between 2006 and 2009 in which Producer Organisations and Producer Milk‐processing Organisations were able to apply for additional production licences. As a consequence, produced output rose and prices fell, particularly in 2008/2009. Since 2009, every milk producer needs to have a contract with a milk buyer. In many cases, the buyer still distinguishes between milk within the former quota boundaries and surplus milk, paying two different prices. Around 50 per cent of milk buyers interviewed in a survey state that their contracts do not determine a fixed price, while the remainder schedule slots with fixed prices of three to twelve months. Most milk buyers manage to maintain a high level of stability in terms of their suppliers: they are neither withdrawing lightly from their existing contracts nor trying to attract large numbers of new suppliers at low prices. The widespread distinction between prices and the commitment of buyers to their former suppliers under the quota regime reveals that the protagonists in the milk market are continuing to think in terms of quotas.
Influence of Environmental Factors on Biotic Responses to Nutrient Enrichment in Agricultural Streams1
Authors: Maret Terry R. , Konrad Christopher P. , Tranmer Andrew W. 
Published: March 2010

Maret, Terry R., Christopher P. Konrad, and Andrew W. Tranmer, 2010. Influence of Environmental Factors on Biotic Responses to Nutrient Enrichment in Agricultural Streams. Journal of the American Water Resources Association (JAWRA) 46(3):498‐513. DOI: 10.1111/j.1752‐1688.2010.00430.xAbstract:  The influence of environmental factors on biotic responses to nutrients was examined in three diverse agricultural regions of the United States. Seventy wadeable sites were selected along an agricultural land use gradient while minimizing natural variation within each region. Nutrients, habitat, algae, macroinvertebrates, and macrophyte cover were sampled during a single summer low‐flow period in 2006 or 2007. Continuous stream stage and water temperature were collected at each site for 30 days prior to sampling. Wide ranges of concentrations were found for total nitrogen (TN) (0.07‐9.61 mg/l) and total phosphorus (TP) (<0.004‐0.361 mg/l), but biotic responses including periphytic and sestonic chlorophyll a (RCHL and SCHL, respectively), and percent of stream bed with aquatic macrophyte (AQM) growth were not strongly related to concentrations of TN or TP. Pearson’s coefficient of determination (R2) for nutrients and biotic measures across all sites ranged from 0.08 to 0.32 and generally were not higher within each region. The biotic measures (RCHL, SCHL, and AQM) were combined in an index to evaluate eutrophic status across sites that could have different biotic responses to nutrient enrichment. Stepwise multiple regression identified TN, percent canopy, median riffle depth, and daily percent change in stage as significant factors for the eutrophic index (R2 = 0.50, p < 0.001). A TN threshold of 0.48 mg/l was identified where eutrophic index scores became less responsive to increasing TN concentrations, for all sites. Multiple plant growth indicators should be used when evaluating eutrophication, especially when streams contain an abundance of macrophytes.
Hydrologic and Morphologic Variability of Streams With Different Cranberry Agriculture Histories, Southern New Jersey, United States1
Authors: Procopio Nicholas A. 
Published: March 2010

Procopio, Nicholas A., 2010. Hydrologic and Morphologic Variability of Streams With Different Cranberry Agriculture Histories, Southern New Jersey, United States. Journal of the American Water Resources Association (JAWRA) 46(3):527‐540. DOI: 10.1111/j.1752‐1688.2010.00432.xAbstract:  The creation of reservoirs and the modification of stream channels are common practices used to facilitate the efficient production of cranberries. The potential impacts to hydrologic and geomorphic aspects of streamflow and channel structure have not been adequately assessed. In this study, the streamflow regime of 12 streams and the channel morphologies of 11 streams were compared for study sites in the Pinelands region of New Jersey with upstream active‐cranberry bogs, upstream abandoned‐cranberry bogs, and basins with no apparent agricultural history. Flow regime metrics included measures of low‐flow, median‐flow, and bankfull discharge, two measures of streamflow variability (spread and a modified Richards‐Baker Flashiness index), and the frequency of overbank flooding. Stream‐channel morphology metrics included average bank slope, average bankfull width, average bankfull depth, average bankfull width‐to‐depth ratio, and average bankfull area. No significant differences between stream types were apparent for any of the metrics. Basin‐area normalized streamflow values of all 12 study sites were highly correlated to each other. Significant relationships existed between some of the flow‐regime and channel‐morphology metrics. Due to the lack of significant differences between stream types, it appears that neither historic nor current cranberry agricultural practices considerably influence flow regimes or the channel morphology of streams in the New Jersey Pinelands.
A Framework for Analyzing Longitudinal and Temporal Variation in River Flow and Developing Flow‐Ecology Relationships1
Authors: Larned Scott T. , Arscott David B. , Schmidt Jochen , Diettrich Jan C. 
Published: March 2010

Larned, Scott T., David B. Arscott, Jochen Schmidt, and Jan C. Diettrich, 2010. A Framework for Analyzing Longitudinal and Temporal Variation in River Flow and Developing Flow‐Ecology Relationships. Journal of the American Water Resources Association (JAWRA) 46(3):541‐553. DOI: 10.1111/j.1752‐1688.2010.00433.xAbstract:  We propose a framework for analyzing longitudinal flow variation and exploring its ecological consequences in four steps: (1) generating longitudinally continuous flow estimates; (2) computing indices that describe site‐specific and longitudinal flow variation, including intermittence; (3) quantifying and visualizing longitudinal dynamics; (4) developing quantitative relationships between hydrological indices and ecological variables (flow‐ecology relationships). We give examples of each step, using data from a New Zealand river and an empirical longitudinal flow model, ELFMOD. ELFMOD uses spot‐gauging data and flow or proxy variable time series to estimate flow magnitude and state (flowing or dry) at user‐defined intervals along river sections. Analyses of flow‐ecology relationships for the New Zealand river indicated that fish and benthic and hyporheic invertebrate communities responded strongly to variation in mean annual flow permanence, flow duration, dry duration, drying frequency, inter‐flood duration, and distances to flowing reaches. To put longitudinal flow variation into a broader context and guide future research, we propose a conceptual model that combines elements of two contrasting perspectives: rivers as longitudinal continua, and rivers as patch mosaics. In this conceptual model, hydrologically complex rivers are composed of linear sequences of nested hydrological gradients, which are bordered by hydrogeomorphic discontinuities, and which collectively generate hydrological dynamics at river‐section scales.
Metabolomics: the chemistry between ecology and genetics
Authors: MACEL MIRKA , Van DAM NICOLE M. , KEURENTJES JOOST J. B. 
Published: March 2010

AbstractMetabolomics is a fast developing field of comprehensive untargeted chemical analyses. It has many applications and can in principle be used on any organism without prior knowledge of the metabolome or genome. The amount of functional information that is acquired with metabolomics largely depends on whether a metabolome database has been developed for the focal species. Metabolomics is a level downstream from transcriptomics and proteomics and has been widely advertised as a functional genomics and systems biology tool. Indeed, it has been successfully applied to link phenotypes to genotypes in the model plant Arabidopsis thaliana. Metabolomics is also increasingly being used in ecology (ecological metabolomics) and environmental sciences (environmental metabolomics). In ecology, the technique has led to novel insights into the mechanisms of plant resistance to herbivores. Some of the most commonly used analytical metabolomic platforms are briefly discussed in this review, as well as their limitations. We will mainly focus on the application of metabolomics in plant ecology and genetics.
Temporal and differential regulation of expression of the eukaryotic‐like ankyrin effectors of Legionella pneumophila
Authors: Al‐Khodor Souhaila , Al‐Quadan Tasneem , Abu Kwaik Yousef 
Published: March 2010

SummaryUpon transition from the exponential (E) to the post‐exponential phase (PE) of growth, Legionella pneumophila undergoes a phenotypic modulation from a replicative to a highly infectious form. This transition requires a delicate regulatory cascade that is triggered to induce expression of various virulence‐related genes. We have recently characterized eleven L. pneumophila eukaryotic‐like ankyrin effectors (Ank) shared between the four sequenced genomes of L. pneumophila. The AnkB effector recruits polyubiquitinated proteins to the Legionella‐containing vacuole (LCV). It is not known whether expression of the ank genes is regulated by various regulators triggered at the PE phase and whether this regulation is essential for function. Here we show that temporal and differential regulation of the ank genes is mediated by RelA, the enhancer protein LetE, and the two component systems LetA/S and PmrA/B. Consistent with the expression of ankB at the PE phase, we show that bacteria grown to the PE but not the E phase recruit polyubiquitinated proteins to the LCV within Acanthamoeba in an AnkB‐dependant mechanism. We conclude that the genes encoding the eukaryotic‐like Ank effectors of L. pneumophila are temporally and spatially regulated at the PE phase.
Emergence of Ciprofloxacin-Nonsusceptible
Streptococcus pyogenes
Isolates from Healthy Children and Pediatric Patients in Portugal
Authors: Pires Renato , Ardanuy Carmen , Rolo Dora , Morais Ana , Brito-Avô António , Gonçalo-Marques José , Liñares Josefina , Santos-Sanches Ilda 
Published: March 2010

ABSTRACT

We describe 66 ciprofloxacin-nonsusceptible
Streptococcus pyogenes
isolates recovered from colonized and infected children. The ParC S79A substitution was frequent and associated with the
emm
6/sequence type 382 (
emm
6/ST382) lineage. The ParC D83G substitution was detected in two isolates (
emm
5/ST99 and
emm
28/ST52 lineages). One isolate (
emm
89/ST101) had no quinolone resistance-determining region codon substitutions or other resistance mechanisms. Five of 66 isolates were levofloxacin resistant. Although fluoroquinolones are not used in children, they may be putative disseminators of fluoroquinolone-nonsusceptible strains in the community.