bluuebunny/crossref_metadata_2025_split huggingface dataset:
The transcription factor D‐Pax2 regulates crystallin production during eye development in Drosophila melanogaster
Authors: Dziedzic Katharine , Heaphy Julie , Prescott Hallie , Kavaler Joshua 
Published: August 2009

AbstractThe generation of a functioning Drosophila eye requires the coordinated differentiation of multiple cell types and the morphogenesis of eye‐specific structures. Here we show that D‐Pax2 plays a significant role in lens development through regulation of the Crystallin gene and because Crystallin is also expressed in D‐Pax2+ cells in the external sensory organs. Loss of D‐Pax2 function leads to loss of Crystallin expression in both eyes and bristles. A 2.3 kilobase (kb) upstream region of the Crystallin gene can drive GFP expression in the eye and is dependent on D‐Pax2. In addition, D‐Pax2 binds to an evolutionarily conserved site in this region that, by itself, is sufficient to drive GFP expression in the eye. However, mutation of this site does not greatly affect the regulatory region's function. The data indicate that D‐Pax2 acts to promote lens development by controlling the production of the major protein component of the lens. Whether this control is direct or indirect remains unresolved. Developmental Dynamics 238:2530–2539, 2009. © 2009 Wiley‐Liss, Inc.
Self‐reported weight gain following smoking cessation: A function of binge eating behavior
Authors: White Marney A. , Masheb Robin M. , Grilo Carlos M. 
Published: August 2009

AbstractObjectiveThis study investigated patterns of self‐reported weight gain following smoking cessation among overweight individuals with and without binge eating.MethodParticipants were 103 overweight (BMI ≥ 25) community volunteers who completed a battery of questionnaires online. Key items queried smoking cessation history and weight gain in the year following cessation. Participants were classified as nonbinge eating overweight (NBO, n = 56) or binge eating disorder (BED, n = 47).ResultsBED participants were significantly more likely to report weight gain in the year following smoking cessation than NBO participants. After controlling for current BMI, the amount of self‐reported weight gain following smoking cessation differed significantly between groups, with the NBO group reporting an average gain of 5.0 kg and the BED group reporting 11.2‐kg gain.DiscussionSince many individuals resume smoking due to cessation‐associated weight gain, these findings highlight the need for targeted interventions for overweight individuals particularly those who also binge eat. © 2009 by Wiley Periodicals, Inc. Int J Eat Disord 2010; 43:572–575
Psychological and behavioral correlates of excess weight: Misperception of obese status among persons with Class II obesity
Authors: Jones Megan , Grilo Carlos M. , Masheb Robin M. , White Marney A. 
Published: August 2009

AbstractObjective:This study examined psychological and behavioral correlates of weight status perception in 173 Class II obese adult community volunteers.Method:Participants completed the Eating Disorder Examination‐Self‐Report (EDE‐Q), Three Factor Eating Questionnaire (TFEQ), Beck Depression Inventory, and Rosenberg Self‐Esteem Scale online. Key items assessed dieting frequency, weight history, and perceived current weight status (normal weight, overweight, or obese). Actual weight status was determined using NIDDK/CDC classification schemes.Results:Among participants with Class II obesity, 50.9% incorrectly classified their weight as overweight versus obese, whereas 49.1% accurately perceived their weight status as obese. Inaccurate participants reported significantly less binge eating and less eating disorder psychopathology. Despite similar BMI, inaccurate participants reported less distress regarding overeating and loss of control over eating.Discussion:Our findings suggest that obesity status underestimation is associated with less eating disorder psychopathology. Underestimation of obesity status may exacerbate risk for negative health consequences because of a failure to recognize and respond to excess weight. © 2009 by Wiley Periodicals, Inc. (Int J Eat Disord 2010;)
Luminescent Polypyridinerhenium(I) Bis‐Biotin Complexes as Crosslinkers for Avidin
Authors: Louie Man‐Wai , Ho‐Chuen Lam Marco , Kam‐Wing Lo Kenneth 
Published: August 2009

AbstractTwo novel luminescent polypyridinerhenium(I) bis‐biotin complexes [Re(NN)(CO)3(pyridine)](CF3SO3) {NN = 4,4′‐bis{[2‐(biotinamido)ethyl]aminocarbonyl}‐2,2′‐bipyridine, bpyC2B2 (1), 4,4′‐bis[(2‐{[6‐(biotinamido)hexanoyl]amino}ethyl)aminocarbonyl]‐2,2′‐bipyridine, bpyC2C6B2 (2)} and their biotin‐free counterpart [NN = 4,4′‐bis(n‐butylaminocarbonyl)‐2,2′‐bipyridine, bpyC4 (3)] have been synthesized and characterized. Upon irradiation, all the complexes exhibited triplet metal‐to‐ligand charge‐transfer (3MLCT) emission in fluid solutions at room temperature and alcohol glass at 77 K. The avidin‐binding properties of the bis‐biotin complexes 1 and 2 have been studied by 4′‐hydroxyazobenzene‐2‐carboxylic acid (HABA) assays, emission titrations, and dissociation assays. The potential use of the complexes as signal amplifiers for heterogeneous recognition assays has been demonstrated using avidin‐coated microspheres and one of the complexes. Additionally, the cytotoxicity of these rhenium(I) complexes towards the human cervix epithelioid carcinoma (HeLa) cell line has been evaluated by 3‐(4,5‐dimethyl‐2‐thiazolyl)‐2,5‐diphenyltetrazolium bromide (MTT) assays. Furthermore, the cellular uptake of the complexes has been examined by laser‐scanning confocal microscopy. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
The Versatile Alkylidene Moiety in Ruthenium Olefin Metathesis Catalysts
Authors: Diesendruck Charles E. , Tzur Eyal , Lemcoff N. Gabriel 
Published: August 2009

AbstractRuthenium olefin metathesis is without doubt one of the most thriving fields in modern synthetic chemistry. Applications in industry and recent developments in basic research make the study of alkylidene ruthenium compounds both exciting and rewarding. As usually recurrent in organometallic catalysis, the development of novel ligands, in particular alkylidene ligands, has had a pivotal role in the tremendous success of the area. This microreview details the evolution of the alkylidene ruthenium compounds, including simple alkylidenes, benzylidenes, Fischer carbenes, vinylidenes, allenylidenes, indenylidenes and the important sub‐class of chelated alkylidenes, and includes a discussion of the significant effects these ligands have had on the precatalysts properties.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Photoluminescent Metal‐Organic Nanotubes via Hydrothermal in Situ Ligand Reactions
Authors: Liu Wen‐Ting , Ou Yong‐Cong , Xie Yu‐Ling , Lin Zhuojia , Tong Ming‐Liang 
Published: August 2009

AbstractHydrothermal reactions of CdCl2, oxalic acid, 1,3,5‐benzenetricarboxylic acid and o‐phenylene diamine (o‐PD) yielded three novel coordination polymers [NaCd4(btc)3(H2bbim)4(H2O)2]·2H2O (1), [KCd4(btc)3(H2bbim)4(H2O)2]·2H2O (2) and [Cd(H2bbim)(H2bimbdc)(HbimbdcH)]·H2O (3) [btc = 1,3,5‐benzene tricarboxylate, H2bbim = 2,2′‐bisbenzimidazole, HbimbdcH2 = 1‐(2‐Benzimidazolyl)‐3,5‐benzene dicarboxylic acid]. The benzimidazole derivatives were in situ generated via hydrothermal metal/ligand condensation of oxalic acid or 1,3,5‐benzenetricarboxylic acid with o‐phenylenediamine. Both 1 and 2 have novel single‐walled nanotubular coordination structures and show strong red photoluminescence at room temperature.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
A Highly Enantioselective Receptor for Carbamoyl Lactic Acid
Authors: Muñiz Francisco M. , Simón Luis , Alcázar Victoria , Raposo César , Fuentes de Arriba Ángel L. , Morán Joaquín R. 
Published: August 2009

AbstractA new receptor based on a 9,9‐dimethylxanthene framework was synthesized. Owing to its suitable oxyanion hole structure, this receptor is able to associate carboxylic acids and anions. The introduction of a chiral center provides enantioselective properties to this receptor as a result of its different interactions with both enantiomers of the substrate. The combination of this skeleton with a fluorescent unit such as dansyl allows the detection of small amounts of carboxylic acids by making use of fluorescent techniques.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Synthesis of Benzazepines by Gold‐Catalysed Reactions of N‐Allenylamides
Authors: González‐Gómez Álvaro , Domínguez Gema , Pérez‐Castells Javier 
Published: August 2009

AbstractThe gold‐catalysed reactions of alleneamides give different products depending on the substrate and the reaction conditions. In particular, N‐(2‐alkynylphenyl)‐N‐allenyltosylamides give benzazepines when using gold(III) catalysts in the presence of nucleophiles. This sequential process may follow two different reaction pathways, and these are discussed. Metal coordination to the alkyne followed by nucleophilic attack of the allene and trapping of the intermediate with NuH accounts for the formation of 4, but coordination to the allene and addition of NuH to give 3, which can decompose into other products and also form 4, is postulated as a better explanation for these results. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
A Flexible Common Approach to α‐Substituted Serines and Alanines: Diastereoconvergent Syntheses of Sphingofungins E and F
Authors: Wang Bing , Lin Guo‐Qiang 
Published: August 2009

AbstractA flexible common approach for the asymmetric syntheses of sphingofungins E and F is reported, with efficient use of both diastereomers of the Baylis–Hillman adduct in a stereoconvergent manner. Pronounced steric effects of the 2‐substituents in diastereoselective dihydroxylations of (E)‐2‐hydroxymethyl‐2,3‐alkenoates were observed. This strategy also allowed full control over the absolute configurations of the quaternary stereocenters in α‐substituted amino acids through tunable site‐specific adjustment of oxidation states.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Abnormal hippocampal shape in offenders with psychopathy
Authors: Boccardi Marina , Ganzola Rossana , Rossi Roberta , Sabattoli Francesca , Laakso Mikko P. , Repo‐Tiihonen Eila , Vaurio Olli , Könönen Mervi , Aronen Hannu J. , Thompson Paul M. , Frisoni Giovanni B. , Tiihonen Jari 
Published: August 2009

AbstractPosterior hippocampal volumes correlate negatively with the severity of psychopathy, but local morphological features are unknown. The aim of this study was to investigate hippocampal morphology in habitually violent offenders having psychopathy. Manual tracings of hippocampi from magnetic resonance images of 26 offenders (age: 32.5 ± 8.4), with different degrees of psychopathy (12 high, 14 medium psychopathy based on the Psychopathy Checklist Revised), and 25 healthy controls (age: 34.6 ± 10.8) were used for statistical modelling of local changes with a surface‐based radial distance mapping method. Both offenders and controls had similar hippocampal volume and asymmetry ratios. Local analysis showed that the high psychopathy group had a significant depression along the longitudinal hippocampal axis, on both the dorsal and ventral aspects, when compared with the healthy controls and the medium psychopathy group. The opposite comparison revealed abnormal enlargement of the lateral borders in both the right and left hippocampi of both high and medium psychopathy groups versus controls, throughout CA1, CA2‐3 and the subicular regions. These enlargement and reduction effects survived statistical correction for multiple comparisons in the main contrast (26 offenders vs. 25 controls) and in most subgroup comparisons. A statistical check excluded a possible confounding effect from amphetamine and polysubstance abuse. These results indicate that habitually violent offenders exhibit a specific abnormal hippocampal morphology, in the absence of total gray matter volume changes, that may relate to different autonomic modulation and abnormal fear‐conditioning. Hum Brain Mapp, 2010. © 2009 Wiley‐Liss, Inc.
Growth fraction as a predictor of response to chemotherapy in node‐negative breast cancer
Authors: Aleskandarany Mohammed A. , Green Andrew R. , Rakha Emad A. , Mohammed Rabab A. , Elsheikh Somaia E. , Powe Desmond G. , Paish Emma C. , Macmillan R. Douglas , Chan Steve , Ahmed Samreen I. , Ellis Ian O. 
Published: August 2009

AbstractAccurate predictive markers of chemotherapeutic response in early breast cancer are still lacking. The role of tumour growth fraction as a predictor of response to chemotherapy was assessed in early breast cancer. In this study, immunohistochemical expression of MIB1 was studied in a well‐characterised series of early (Stages I and II) node‐negative breast carcinoma cases (n = 100) with long‐term follow‐up that have received adjuvant chemotherapy (cyclophosphamide/methotrexate/5‐fluorouracil regimen). In addition, 728 cases who did not receive adjuvant chemotherapy were used as a control group. Increased tumour growth fraction was associated with a better response to adjuvant chemotherapy in terms of longer breast cancer specific survival and disease‐free interval [hazard ratio (HR) = 0.354, 95% CI = 0.177–0.688, p = 0.003 and HR = 0.396, 95% CI = 0.205–0.768, p = 0.006, respectively]. In contrast to the control group, patients with high growth fraction tumour (>70%) showed an excellent outcome with infrequently reported events during the period of follow‐up. Importantly, patients with a low growth fraction (≤10%) showed frequent recurrences and shorter survival time with outcome comparable to those of high growth fraction who did not receive chemotherapy. Therefore, tumour growth fraction can be used to assign patients into distinct groups showing differential response to adjuvant chemotherapy. Patients with a high growth fraction appear to be ideal candidates for adjuvant chemotherapy while those with low growth fraction are less likely to benefit and are prone to the potential serious side effects of adjuvant chemotherapy.
Feasibility of chemohyperthermia with docetaxel‐embedded magnetoliposomes as minimally invasive local treatment for cancer
Authors: Yoshida Motohira , Watanabe Yuji , Sato Mitsunori , Maehara Tsunehiro , Aono Hiromichi , Naohara Takashi , Hirazawa Hideyuki , Horiuchi Atsushi , Yukumi Shungo , Sato Koichi , Nakagawa Hiromichi , Yamamoto Yuji , Sugishita Hiroki , Kawachi Kanji 
Published: August 2009

AbstractHyperthermia is a minimally invasive approach to cancer treatment, but it is difficult to heat only the tumor without damaging surrounding tissue. To solve this problem, we studied the effectiveness of chemohyperthermia with docetaxel‐embedded magnetoliposomes (DMLs) and an applied alternating current (AC) magnetic field. Human MKN45 gastric cancer cells were implanted in the hind limb of Balb‐c/nu/nu mice. Various concentrations of docetaxel‐embedded DMLs were injected into the tumors and exposed to an AC magnetic field (n = 6, each). For comparison with hyperthermia alone, magnetite‐loaded liposome (ML)‐injected tumors were exposed to an AC magnetic field. Furthermore, the results of DML without AC treatment and docetaxel diluted into PBS with AC treatment were also compared (n = 10, each). Tumor surface temperature was maintained between 42 and 43°C. Tumor volume was reduced in the DML group with a docetaxel concentration > 56.8 μg/ml, while a docetaxel concentration > 568.5 μg/ml was required for tumor reduction without hyperthermia. Statistically significant differences in tumor volume and survival rate were observed between the DML group exposed to the magnetic field and the other groups. The tumor disappeared in 3 mice in the DML group exposed to the magnetic field; 2 mice survived over 6 months after treatment, whereas all mice of the other groups died by 15 weeks. Histologically, hyperthermia with DML damaged tumor cells and DML diffused homogeneously. To the best of our knowledge, this is the first report to show that hyperthermia using chemotherapeutic agent‐embedded magnetoliposomes has an anticancer effect.
Library screening by fragment‐based docking
Authors: Huang Danzhi , Caflisch Amedeo 
Published: August 2009

AbstractWe review our computational tools for high‐throughput screening by fragment‐based docking of large collections of small molecules. Applications to six different enzymes, four proteases, and two protein kinases, are presented. Remarkably, several low‐micromolar inhibitors were discovered in each of the high‐throughput docking campaigns. Probable reasons for the lack of submicromolar inhibitors are the tiny fraction of chemical space covered by the libraries of available compounds, as well as the approximations in the methods employed for scoring, and the use of a rigid conformation of the target protein. Copyright © 2009 John Wiley & Sons, Ltd.
1H spectroscopic imaging of human brain at 3 Tesla: Comparison of fast three‐dimensional magnetic resonance spectroscopic imaging techniques
Authors: Zierhut Matthew L. , Ozturk‐Isik Esin , Chen Albert P. , Park Ilwoo , Vigneron Daniel B. , Nelson Sarah J. 
Published: August 2009

AbstractPurposeTo investigate the signal‐to‐noise‐ratio (SNR) and data quality of time‐reduced three‐dimensional (3D) proton magnetic resonance spectroscopic imaging (1H MRSI) techniques in the human brain at 3 Tesla.Materials and MethodsTechniques that were investigated included ellipsoidal k‐space sampling, parallel imaging, and echo‐planar spectroscopic imaging (EPSI). The SNR values for N‐acetyl aspartate, choline, creatine, and lactate or lipid peaks were compared after correcting for effective spatial resolution and acquisition time in a phantom and in the brains of human volunteers. Other factors considered were linewidths, metabolite ratios, partial volume effects, and subcutaneous lipid contamination.ResultsIn volunteers, the median normalized SNR for parallel imaging data decreased by 34–42%, but could be significantly improved using regularization. The normalized signal to noise loss in flyback EPSI data was 11–18%. The effective spatial resolutions of the traditional, ellipsoidal, sensitivity encoding (SENSE) sampling scheme, and EPSI data were 1.02, 2.43, 1.03, and 1.01 cm3, respectively. As expected, lipid contamination was variable between subjects but was highest for the SENSE data. Patient data obtained using the flyback EPSI method were of excellent quality.ConclusionData from all 1H 3D‐MRSI techniques were qualitatively acceptable, based upon SNR, linewidths, and metabolite ratios. The larger field of view obtained with the EPSI methods showed negligible lipid aliasing with acceptable SNR values in less than 9.5 min without compromising the point‐spread function. J. Magn. Reson. Imaging 2009;30:473–480. © 2009 Wiley‐Liss, Inc.
Rapid isotropic 3D‐sodium MRI of the knee joint in vivo at 7T
Authors: Wang Ligong , Wu Yan , Chang Gregory , Oesingmann Niels , Schweitzer Mark E. , Jerschow Alexej , Regatte Ravinder R. 
Published: August 2009

AbstractPurposeTo demonstrate the feasibility of acquiring high‐resolution, isotropic 3D‐sodium magnetic resonance (MR) images of the whole knee joint in vivo at ultrahigh field strength (7.0T) via a 3D‐radial acquisition with ultrashort echo times and clinically acceptable acquisition times.Materials and MethodsFive healthy controls (four males, one female; mean ± standard deviation [SD] age 28.7 ± 4.8 years) and five patients with osteoarthritis (OA) (three males, two females; mean ± SD age 52.4 ± 5.6 years) underwent 23Na MRI on a 7T, multinuclei equipped whole‐body scanner. A quadrature 23Na knee coil and a 3D‐gradient echo (GRE) imaging sequence with a radial acquisition were utilized. Cartilage sodium concentration was measured and compared between the healthy controls and OA patients.ResultsThe average signal‐to‐noise ratio (SNR) for different spatial resolutions (1.2–4 mm) varied from ∼14–120, respectively. The mean sodium concentration of healthy subjects ranged from ∼240 ± 28 mM/L to 280 ± 22 mM/L. However, in OA patients the sodium concentrations were reduced significantly by ∼30%–60%, depending on the degree of cartilage degeneration.ConclusionThe preliminary results suggest that sodium imaging at 7T may be a feasible potential alternative for physiologic OA imaging and clinical diagnosis. J. Magn. Reson. Imaging 2009;30:606–614. © 2009 Wiley‐Liss, Inc.
Selective molecularly imprinted stationary phases for Bisphenol A analysis prepared by modified precipitation polymerization
Authors: Jiang Ming , Shi Yun , Zhang Ren‐li , Shi Chang‐hua , Peng Yan , Huang Zheng , Lu Bin 
Published: August 2009

AbstractBisphenol A (BPA)‐imprinted polymeric microspheres were synthesized by modified precipitation polymerization (MPP) method. Influences of cross‐linker, monomer, porogen volume, and agitation on polymerization were investigated. Proper amount of cross‐linker ethyleneglycol‐dimethacrylate (EGDMA) was critical to achieve narrowly dispersed microspheres. For template BPA, monomer 4‐vinylpyridine (4‐VP) was better than MAA to get the best imprinted effects. The optimum template/monomer ratio was 1:6. Increasing porogen volume increased size dispersity and decreased binding characters. Agitation increased coagulation and resulted in irregular particles. Microspheres with the best binding characters were used as selective stationary phase of chromatographic column to detect BPA in milk, pig urine, and chicken meat. Under optimal chromatographic conditions, the calibration graph was linear with R2 = 0.9994 in the range of 3–50 μmol/L. The LOD and LOQ were 1 and 3 μmol/L, respectively. When large amounts (20 mL or 20 g) of samples were analyzed, the recoveries ranged from 70.2 to 87.3% with RSD less than 4.85% in all samples spiked with 0.05–0.2 μmol/L BPA. The intra‐day and inter‐day RSD were less than 1.83 and 3.96%, respectively. Microspheres prepared by MPP are successfully used in molecularly imprinted polymer (MIP)‐based analytical column to detect trace BPA in different biologic samples with acceptable accuracy and repeatability.
Impact of surveillance on survival after laryngeal cancer in the medicare population
Authors: Francis David O. , Yueh Bevan , Weymuller Ernest A. , Merati Albert L. 
Published: August 2009

AbstractObjectives/Hypothesis:Routine surveillance is advocated to detect recurrent disease after treatment for laryngeal cancer. This aim of this study was to determine the 1‐ and 5‐year postrecurrence mortality for laryngeal cancers and evaluate whether more intensive surveillance improved survival.Study Design:Retrospective cohort study.Methods:Patients with recurrent cancers (1992–1999) were identified in a national cancer clinical database. Multivariate analysis was used to evaluate the effect of surveillance on postrecurrence survival.Results:Of 2,121 recurrent cancers identified, 913 were laryngeal. Patients with laryngeal cancer recurrence had 27% (P = .001) and 22% (P = .007) better odds of 1‐ and 5‐year survival than other sites. The 1‐ and 5‐year postrecurrence survival rates for laryngeal cancer patients were 72.4% and 41.3%, respectively. Glottic cancer cases had the best postrecurrence life expectancy. Multivariate regression revealed that clinical surveillance intensity had no independent impact on their survival (P < .05). However, patients with recurrent glottic cancer seen in surveillance had 23% improved odds of survival (P = .037).Conclusions:More frequent surveillance visits was not associated with a survival advantage in the overall population. Patients with glottic cancer had a postrecurrence survival advantage if seen during the surveillance period. Laryngeal cancer patients had better postrecurrence survival than other head and neck sites. Laryngoscope, 2009