bluuebunny/crossref_metadata_2025_split huggingface dataset:
In vitro Propagation of Bitter Gourd (Momordica charantia L.) from Nodal and Root Segments
Authors: Munsur M.A.Z. Al , Haque M.S. , Nasiruddin K.M. , Hossain M.S. 
Published: July 2010

Explants of nodal and root segments of bitter gourd were cultured on MS supplemented with various concentrations of BAP in combination with either 2,4-D or NAA. Nodal segments produced the highest percentage (93.75) of callus in MS supplemented with 1.0 mg/l 2,4-D and 1.0 mg/l BAP whereas, root segments produced the highest (85.00%) callus in 0.6 mg/l NAA and 2.5 mg/l BAP combination. A combination of 1.0 mg/l 2,4-D and 1.0 mg/l BAP exhibited 75.00% shoot regeneration from nodal segments. The highest shoot length (5.15 cm) was recorded with 2.5 mg/l BAP and 0.2 mg/l IAA from nodal segments. No sign of regeneration of shoot was found from root segments in any of the combinations. However, some combinations produced only roots. Key words: In vitro propagation, bitter gourd, nodal segments, Root segments D.O.I. 10.3329/ptcb.v19i1.4916 Plant Tissue Cult. & Biotech. 19(1): 45-52, 2009 (June)
Clonal Propagation Through Nodal Explant Culture of Boerhaavia diffusa L. - A Rare Medicinal Plant
Authors: Biswas A. , Bari M. A. , Roy M. , Bhadra S. K. 
Published: July 2010

Multiple shoots were induced on MS fortified with 2.0 mg/l BAP and 0.2 mg/l NAA within 30 days of culture. Maximum (93%) explants produced multiple shoots with an average 12 shoots per culture after two successive subcultures at 14 days interval in the same medium. Cent per cent rooting was achieved in half strength of MS supplemented with 1.0 mg/l IBA. Following 21 days in vitro rooting period and seven days of ex vitro acclimatization, plantlets were successfully established in natural condition. The survival rate of regenerated plants was 90 per cent. Key words: Clonal propagation, Boerhaavia diffusa, Rare medicinal plant, Nodal culture D.O.I. 10.3329/ptcb.v19i1.4917 Plant Tissue Cult. & Biotech. 19(1): 53-59, 2009 (June)
In vitro Clonal Propagation of Anthurium (Anthurium andraeanum L.) Using Callus Culture
Authors: Jahan M.T. , Islam M.R. , Khan Ruseli , Mamun A.N.K. , Ahmed G. , Hakim L. 
Published: July 2010

High frequency of calli was obtained from leaf and spadix segments of Anthurium andraeanum L. when cultured on N6 medium containing 2.5 mg/l BAP and 0.2 mg/l 2,4-D in dark condition. The calli were maintained in dark condition on the same medium containing same hormonal supplements by sub-culturing up to three months but no shoot formation occurred during this period. Leaf and spadix segments derived calli were then tried for multiple shoot regeneration by culturing onto MS fortified with BAP and Kn singly or in combination with NAA in light condition. Best response towards multiple shoot regeneration was observed from both leaf and spadix segments derived calli on MS fortified with 1.0 mg/l BAP. In this combination an average of 18 shoot buds regenerated from leaf segment derived callus while 14 shoot buds regenerated from spadix segment derived callus. The number of shoot buds increased three to four-folds after subculturing in the same medium. Regenerated multiple shoots were excised and cultured onto half strength of MS with different concentrations of IBA and IAA for root induction. Best root development was obtained in half strength MS containing 1.0 mg/l IBA. About 85 per cent of the regenerated plantlets survived in natural conditions. Key words: Clonal propagation, In vitro, Anthurium andraeanum D.O.I. 10.3329/ptcb.v19i1.4961 Plant Tissue Cult. & Biotech. 19(1): 61-69, 2009 (June)
Plant Regeneration from Leaf Explants of Plumbago
Authors: Gopalakrishnan M. , Janarthananm B. , Lakshmi Sai G. , Sekar T. 
Published: July 2010

Leaf explants of Plumbago rosea L. an important medicinal plant inoculated on MS supplemented with 6.66 mM BAP and 2.69 mM NAA produced numerous (105 ± 0.3) shootlets with an average length of 3.1 ± 0.0 cm. Small shootlets were transferred to shoot elongation medium supplemented with 1.11 mM BAP plus 1.44 mM GA3. The elongated shootlets transferred to half strength MS basal medium without any plant growth regulator produced 4.3 ± 0.2 rootlets per plants with average root length of 4.0 ± 0.0 cm after 25 days of culture. Rooted plantlets were transferred for hardening showed 80 - 90 per cent of plantlets success-fully established in the field. Potentially more than 50,000 plantlets could be produced within five subcultures from without callus phase obtained from leaf explant. Maximum root differentiation from leaf explants was obtained on MS supplemented with 5.38 mM IBA. The roots developed by the above method is an alternative for the controlled production of secondary metabolites. Key words: In vitro culture, leaf explants, mass propagation, Plumbago rosea D.O.I. 10.3329/ptcb.v19i1.4989 Plant Tissue Cult. & Biotech. 19(1): 79-87, 2009 (June)
Establishment of Morphogenic and Regeneration Ability in two Indica Rice Cell Suspension Cultures after Exposure to NaCl
Authors: Aditya T.L. 
Published: July 2010

An efficient protocol was developed for in vitro morphogenic ability along with plantlet regeneration of two Bangladeshi indica rice varieties (BR24 and BR26) via somatic embryogenesis by applying 50 mM NaCl stress in callus induction and suspension initiation media. Osmotic stress was induced by NaCl (50, 100, 150, 200 and 250 mM) on the cell growth in suspension maintenance media. In viability test stress adapted cells showed 85 - 95% viability up to 200 mM NaCl compared with stress shocked (MS1-50) and control (MS1-0) treatments. Higher stress adapted cells showed growth retardation and the induction of plasmolysis. For both genotypes somatic embryos were obtained in both MS based liquid and semisolid media with or without 50 and 100 mM NaCl. Cell suspension-derived micro-calli were partially desiccated (6 - 12 hr) and subsequently maintained in MS1 callus induction media supplemented with proline (12 mM), ABA (2 mg/l) and 0.6% phytagel in the presence or absence of 50 and 100 mM NaCl. Subsequently, desiccated somatic embryos were transferred in MS based regeneration media with or without 50 and 100 mM NaCl. Proline mediated callus was found to be more effective in embryo differentiation than ABA. Partial desiccation dramatically enhanced callus growth and partially increased regeneration percentage. BR24 showed a better regeneration response producing plantlets in presence of proline in control media while BR26 restored regeneration potential in the presence of ABA and 100 mM NaCl. Plantlets regenerated from salt stressed callus cultures were then grown in compost in a glasshouse and produced normal, fertile plants. Key words: Indica rice, Cell suspension, Morphogenic, Regeneration D.O.I. 10.3329/ptcb.v19i2.5436 Plant Tissue Cult. & Biotech. 19(2): 185-197, 2009 (December)
Sequence Specific Dihedral Angle Distribution: Application in Protein Structure Prediction and Evaluation
Authors: Minhaz Ud-Dean S. M. , Moosa Mahdi Muhammad 
Published: July 2010

Protein structure prediction and evaluation is one of the major fields of computational biology. Estimation of dihedral angle can provide information about the acceptability of both theoretically predicted and experimentally determined structures. Here we report on the sequence specific dihedral angle distribution of high resolution protein structures available in PDB and have developed Sasichandran, a tool for sequence specific dihedral angle prediction and structure evaluation. This tool will allow evaluation of a protein structure in pdb format from the sequence specific distribution of Ramachandran angles. Additionally, it will allow retrieval of the most probable Ramachandran angles for a given sequence along with the sequence specific data. Key words: Torsion angle, φ-ψ distribution, sequence specific ramachandran plot, Ramasekharan, protein structure appraisal D.O.I. 10.3329/ptcb.v19i2.5439 Plant Tissue Cult. & Biotech. 19(2): 217-226, 2009 (December)
Precise Incubation Period for the Agrobacterium- mediated Transformation Efficiency in Potato (Solanum tuberosum L.) cvs. Cardinal and Atlas
Authors: Sarker S. R. , Hossain M. , Shirin F. 
Published: July 2010

Precise incubation period facilitates significant response to Agrobacterium- mediated genetic transformation of potato (Solanum tuberosum L.) cvs. Atlas and Cardinal. The protocol yielded an average transformation rate of 58.4% for internodal explants compared to 38% for leaf explants in Atlas while 47.6% for intenodal explants as against 7.6% for leaf explants in Cardinal. Highest survival rate and transient GUS activity were shown by Atlas and Cardinal at 45 min of incubation with Agrobacterium. The plants were analyzed histochemically for GUS activity in their leaves and internodes. All these analysis indicated that each independently selected and regenerated plants of Atlas and Cardinal were GUS positive and transient. Key words: Transformation, Incubation period, Potato cultivars D.O.I. 10.3329/ptcb.v19i2.5440 Plant Tissue Cult. & Biotech. 19(2): 227-235, 2009 (December)
Effect of Bavistin and Adenine Sulphate on In vitro Shoot Multiplication of Picrorhiza scrophulariiflora
Authors: Bantawa Pranay , Roy Olivia Saha , Ghosh Parthadeb , Mondal Tapan Kumar 
Published: July 2010

An alternative protocol for in vitro propagation of Picrorhiza scrophulariiflora is described using bavistin and adenine sulphate. The explants differentiated into multiple shoot buds on MS supplemented with various concentrations of bavistin and adenine sulphate ranging from 0 - 400 mg/l either alone or in combination. Maximum number of multiple shoots were obtained on MS containing the combination of bavistin (100 mg/l) and adenine sulphate (100 mg/l). In this combination as high as 28 shoots per explant was achieved and also vetrification of the cultures were not recorded. This study also demonstrates that the bavistin has stronger cytokinin-like activity than adenine sulphate. For instance, it was observed that bavistin alone in the concentration of 300 mg/l produced as high as 24 shoots per explant, however, adenine sulphate (100 mg/l) could produce a maximum of 18 shoots per explant. Moreover, higher or lower concentration did not improve the shoot multiplication. The microshoots were separated from the multiple shoots and transferred to MS containing various concentrations of auxins. Among them, NAA (1 mg/l) produced as high as 6 roots per explant. The regenerated plantlets were hardened in plastic cups (6 x 8 cm) containing 9 : 1 virgin soil and soil at Kyongnosla nursery and acclimated for four weeks. A 90% survival rate of the plants was recorded after 60 days. D.O.I. 10.3329/ptcb.v19i2.5441 Plant Tissue Cult. & Biotech. 19(2): 237-245, 2009 (December)
Rapid In vitro Root Induction in Transgenic
Authors: Rashid Bushra , Husnain Tayyab , Riazuddin S. 
Published: July 2010

Key words: Gossypium hirsutum, Rooting, Transgenic cotton D.O.I. 10.3329/ptcb.v19i2.5442 Plant Tissue Cult. & Biotech. 19(2): 247-251, 2009 (December) - Short communication
Field layout of a tiltmeter array to monitor micro‐deformation induced by pumping through a horizontal collector well
Authors: Chen H.C. , Kümpel H.‐J. , Krawczyk C.M. 
Published: July 2010

ABSTRACTPump‐induced tilt deformation in the subsurface has repeatedly been observed as a hydromechanical, poroelastic response to changes in pump rates in the vicinity of fluid‐producing wells. Within the Fuhrberg waterworks area, north of Hannover, Germany, groundwater is withdrawn from 22 m depth through a system of horizontal wells out of a sandy, unconfined aquifer. To measure the pump‐induced tilt, a specific field layout was designed and a unique data set recorded. The monitoring experiment was set in place by installing 12 borehole tiltmeters at depths of approximately 2 m below surface. We monitored micro‐deformation signals in situ for up to two years, so that long‐term, short‐term and event‐type tilt signals could be identified. During the study, the pump rate of the producing well system varied from 50–400 m3/h in an irregular but controlled manner. The field layout of the tiltmeter array allowed to trace both the overall signature of the induced micro‐deformations around the well system as well as the tilt responses at individual tiltmeter positions. For a variation of the pump rate by 100 m3/h we found the average amplitude of the induced signal to be around . There is a general trend for the induced tilt signals to build up in a concentric way around the central pit. Nevertheless, there are significant deviations from the expected tilt response, namely of order ±10° in azimuth or in amplitude per 100 m3/h change in pump rate for roughly half of all the 42 tiltmeter sites monitored. The deviating tilt azimuths are believed to reflect the presence of heterogeneities in the glacio‐fluviatile sediments at this location. While influence of the local topography is of minor importance, as well as noise from meteorological and hydrological causes, effects due to the nearby vegetation can mask the pump‐induced tilt signal by up to 30%. Here, the distance of the observation points away from larger trees is essential, rather than the overall amount of vegetation.
EPSAC‐controlled anesthesia with online gain adaptation
Authors: Niño J. , De Keyser R. , Syafiie S. , Ionescu C. , Struys M. 
Published: July 2010

AbstractThis paper presents the application of predictive control to drug dosing during anesthesia in patients undergoing surgery. A single‐input (propofol) single‐output (bispectral index (BIS)) model of the patient has been assumed for prediction. The performance of our previous strategy in drug dosing control has been improved to tackle inter‐patient variability. A set of 12 patient models was studied and, in order to ensure the applicability of the proposed controller, gain adaptation in the controller is proposed. Preliminary studies have shown that due to static nonlinearity in the sigmoid curve of the patient model, feedback control is not feasible in the first part of the induction phase. Therefore, the control strategy applied in this study consists of controlling the effect site concentration (Ce) during the first phase and controlling BIS once the relation BIS/Cehas been identified. The policy of switching and adapting the control strategies shows a good performance during the induction phase in simulation studies. Clinical tests have been scheduled at the Ghent University Hospital for the coming months. Copyright © 2008 John Wiley & Sons, Ltd.
Chemisorption of hydrogen sulfide on halloysite‐based porous clay heterostructures modified with potassium permanganate
Authors: Lu Zhenxiang , Huang Zheng‐Hong , Kang Feiyu 
Published: July 2010

ABSTRACTHydrogen sulfide gas is harmful to human health and causes corrosion of electronic devices, when it is present in indoor air. Porous clay heterostructures (PCHs) provide a new type of material for catalysis, adsorptive separations and as H2S sensors because their large pores allow access to their active interiors. PCHs posses a large adsorptive surface area (400–900 m2 g−1) and a unique combination of micro‐ and mesoporosity. In this work, PCHs were synthesized by the polymerization of tetraethoxysilane (TEOS) in the micelles, which were assembled by mixing tubular halloysite powders and cetyltrimethylammonium bromide (CTMAB). Both as‐synthesized PCHs and pristine halloysite were impregnated with KMnO4 by equal‐volume mixing to obtain chemosorbents. The mass percentages of KMnO4 ranged from 5 to 30 wt%. The samples were characterized by using N2 adsorption, X‐ray diffraction (XRD), diffuse reflectance ultraviolet–visible (UV–Vis) spectroscopy, transmission electron microscope (TEM) and infrared spectroscopy. The dynamic adsorption of the chemosorbents for hydrogen sulfide was evaluated with a fixed bed. The results show that the PCH‐based chemosorbents exhibit better H2S breakthrough performance due to their rich porous structure, the samples with 15–20 wt% KMnO4 possessing the highest activity. This study shows that the KMnO4/PCH chemosorbents can effectively remove H2S from indoor air at room temperature. © 2010 Curtin University of Technology and John Wiley & Sons, Ltd.
Construct biomimetic giant vesicles via self‐assembly of poly(2‐methacryloyloxyethyl phosphorylcholine)‐block‐poly(D,L‐lactide)
Authors: Liu Gongyan , Hu Xiaofen , Chen Chaojian , Ji Jian 
Published: July 2010

AbstractThe poly(2‐methacryloyloxyethyl phosphorylcholine)‐block‐poly(D,L‐lactide) (PMPC‐b‐PLA) was specially designed to develop biomimetic giant vesicles (GVs) and giant large compound vesicles via a simple spontaneous assemble in aqueous solution. The weight fraction of the hydrophilic PMPC block (fPC) was proved to play an important role in the size and morphology control of the self‐assembled aggregates. The GVs with controlled micrometer size and biomimetic PMPC corona have great potential as artificial cell models. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010
Effect of prepolyurethane on the performances of poly(acrylates‐co‐urethane) copolymer
Authors: Li Minrui , Zheng Zhen , Liu Shujuan , Wei Wei , Wang Xinling 
Published: July 2010

AbstractPoly(acrylates‐co‐urethane) copolymers were synthesized by the polymerization of acrylates and 2‐hydroxyethyl acrylate terminated polyurethane (prepolyurethane) at room temperature. The polymerization was initiated by the radicals, which were produced by the oxidation of tri‐n‐butylborane (TBB) from the mixing of TBB/hexamethylene diamine complex and diisocyanate decomplexer. The effects of prepolyurethane on the performances of copolymers were discussed. The results indicated that the damping property and flexibility of copolymers were obviously higher than that of pure polyacrylates. Dynamic mechanical analysis and transmission electron microscope results showed that PU was dispersed in polyacrylates phase very well. The copolymers were found to bond well to low surface energy materials because of introduction of PU. The lap shearing strength of copolymer bonding polypropylene or polyethylene both had the trend of first increase followed by decrease with the prepolyurethane content increase. The copolymers as adhesives were also shown to have a long working life and be suitable for utilization at low temperature especially at room temperature. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010
Infrared spectra and conformation of monodisperse Oligo‐γ‐Benzyl‐L‐Glutamates in solution
Authors: Imae Toyoko , Ikeda Shoichi 
Published: July 2010

AbstractThe ir spectra of σ–nitrophenylthio‐tetra‐ and hexa‐γ‐benzyl‐L‐glutamate ethylamides were measured at different concentrations in chloroform and ethylene dichloride. The molar extinction coefficients of two bands, each for the amide I and A modes, were observed as indicating the content of β structure and the fraction of hydrogen bonds; they were analyzed for elucidating the hydrogen‐bonded state of peptide residues in the σ and β conformations of oligopeptides. While the content of β structure of the tetra‐peptide increases with increasing concentration, the hexapeptide is in the β conformation above the critical concentration only. The fraction of hydrogen bonds remains finite even at infinite dilution or below the critical concentration, indicating the intramolecular hydrogen‐bonding in the σ—form. As the fundamental structure of folded forms having only intramolecular hydrogen bonds, the 27 ribbon is most likely. With increasing concentration or above the critical concentration, the extended forms are stabilized by the intermolecular hydrogen bonds between residues of the β‐form. The β‐form is present only when intermolecular hydrogen bonds link two residues in an antiparallel way. Possible structures of the oligopeptides in the σ– and β conformations in the two solvents are described briefly.
The Resolution of nucleic acid‐ligand binding sites by CD first‐neighbor analysis
Authors: Allen Fritz S. , Gray Donald M. 
Published: July 2010

AbstractThe first‐neighbor approximation for representation of double‐stranded polynucleotide CD has been used to determine which first‐neighbor units are favorable binding sites for a ligand. Here, we refine the process to yield a more definitive answer. The binding at the first‐neighbor units, ApA:TpT and CpC:GpG, may be unequivocally resolved, and when fewer than six of the remaining eight base‐paired first‐neighbor units are bound by the ligand, they too can be resolved. If this condition is not met, then the binding can be characterized as nonspecific.
Highway design software as support of a project‐based learning course
Authors: Castro Maria 
Published: July 2010

AbstractTraditionally, practical training of students in highway design has been based on solving geometric problems without using professional highway design software. In a degree of the Technical University of Madrid (UPM), teaching of this subject has been redesigned using a project‐based learning approach. Along the course, the students make a complete project of a highway using professional software and procedures. The highway design software chosen includes a simulator that makes a virtual journey along the highway from the point of view of a driver traveling on it. A detailed description of the course program and the software used is provided. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 20: 468–473, 2012
Ureterocalicostomy to Salvage a Renal Allograft with Pyeloureteral Necrosis
Authors: Pais Vernon M. , Clark Curtis J. , Lucas Bruce A. 
Published: July 2010

AbstractNecrosis of an allograft's renal pelvis and ureter is an uncommon but potentially devastating complication of renal transplantation. Although its occurrence may result in significant morbidity—including graft loss—or mortality, reconstruction of the collecting system may save the transplanted kidney. We present a case of successful renal allograft salvage despite necrosis of the renal pelvis and ureter, utilizing a modified ureterocalicostomy.
Erläuterungen zur “DIBt‐Richtlinie zur Anwendung von DIN V ENV 1994‐1‐2 in Verbindung mit DIN 18800‐5”
Authors: Hothan S. 
Published: July 2010

AbstractAuf der 169. Sitzung der Fachkommission Bautechnik am 28.02. und 01.03.2007 in Berlin wurde die Aufnahme von DIN 18800‐5:2007‐03 in die Muster‐Liste der Technischen Baubestimmungen beschlossen. Diese Verbundbaunorm ersetzt die Vornorm DIN V 18800‐5:2004‐11. Als Grundlage für die brandschutztechnische Bemessung und Klassifizierung von Verbundbauteilen wird in DIN 18800‐5:2007‐03 auf die “DIBt‐Richtlinie zur Anwendung von DIN V ENV 1994‐1‐2 in Verbindung mit DIN 18800‐5” verwiesen. Diese Richtlinie ermöglicht anhand von DIN V ENV 1994‐1‐2:1997‐07 in Verbindung mit dem zugehörigen Nationalen Anwendungsdokument (DIN‐Fachbericht 94) die brandschutztechnische Einstufung von Verbundbauteilen, deren Bemessung bei Normaltemperatur nach DIN 18800‐5:2007‐03 durchgeführt wurde.
Sfrp1, Sfrp2, and Sfrp5 regulate the Wnt/β‐catenin and the planar cell polarity pathways during early trunk formation in mouse
Authors: Satoh Wataru , Matsuyama Makoto , Takemura Hiromasa , Aizawa Shinichi , Shimono Akihiko 
Published: July 2010

AbstractThe secreted frizzled‐related protein gene family encodes proteins that regulate Wnt signaling. Msx1 in situ hybridization of 9.5 days post coitus mouse embryos showing normal neural tube development in an Sfrp1; Sfrp2 double mutant (left) but severe neural tube defects in a Looptail (Lp/+); Sfrp1; Sfrp2 triple mutant (right). These findings suggest that Sfrps regulate the Wnt planar cell polarity pathway. See Satoh et al. in this issue.