bluuebunny/crossref_metadata_2025_split huggingface dataset:
Does serum carcinoembryonic antigen elevation in patients with postoperative stage II colorectal cancer indicate recurrence? Comparison with stage III
Authors: Hara Masayasu , Sato Mikinori , Takahashi Hiroki , Takayama Satoru , Takeyama Hiromitsu 
Published: May 2010

AbstractBackground and ObjectivesThe aim of this study was to determine the accuracy of postoperative monitoring of serum carcinoembryonic antigen (CEA) to detect or rule out recurrence in patients with stage II colorectal cancer (CRC) by comparing results with stage III.MethodsA total of 303 patients with CRC who underwent curative surgery were enrolled. Serum CEA was assayed, and radiological examination was performed routinely for 5 years after surgery. Yearly recurrence rates, sensitivities, specificities, likelihood ratios, and posttest probabilities were calculated.ResultsSensitivity and specificity of CEA monitoring in stage II patients are almost same as those in stage III. Whereas recurrences occurred early in stage III, they occurred almost as frequently in both early and late stage II. The obtained posttest probability of recurrence in stage II patients with CEA elevation was significantly lower (only 30% or less) than those in stage III (approximately 80%).ConclusionElevation of CEA in patients with stage II CRC does not represent recurrence with high probability. One of the reasons for the unreliability of CEA monitoring was its high false‐positive rate. Another tumor marker with a lower false‐positive rate is necessary to follow‐up stage II CRC patients. J. Surg. Oncol. 2010;102:154–157. © 2010 Wiley‐Liss, Inc.
Outcome of selected breast cancer patients with micrometastasis or isolated tumor cells in sentinel node biopsy and no completion axillary lymph node dissection
Authors: Meretoja Tuomo J. , Vironen Jaana H. , Heikkilä Päivi S. , Leidenius Marjut H. 
Published: May 2010

AbstractBackground and ObjectivesAxillary lymph node dissection (ALND) is the standard of care in patients with tumor‐positive sentinel nodes (SN). However, approximately half of these patients do not have additional metastases in their axilla and therefore do not benefit from completion ALND. Our aim was to examine the outcome of highly selected breast cancer patients with tumor‐positive SN without completion ALND.MethodsAltogether 48 patients with tumor‐positive SN without ALND were included in this study. Twenty‐two patients had micrometastasis and 26 had isolated tumor cells (ITC) in their sentinel node biopsy. The median follow‐up time was 37 months (range 9–78).ResultsNo axillary recurrences occurred during the follow‐up. One patient had a local recurrence. Distant metastases as the first event were observed in two patients. One of them died in breast cancer. Nine patients died from intercurrent causes.ConclusionsOmitting ALND seems safe in selected breast cancer patients with SN micrometastasis or ITC. J. Surg. Oncol. 2010;102:215–219. © 2010 Wiley‐Liss, Inc.
Evaluation of selective composite cryogel for bromate removal from drinking water
Authors: Hajizadeh Solmaz , Kirsebom Harald , Galaev Igor Y. , Mattiasson Bo 
Published: May 2010

AbstractBromate, which is a potential carcinogen, should be removed from drinking water to levels of less than 10 μg/L. A chitosan‐based molecularly imprinted polymer (MIP) and a sol–gel ion‐exchange double hydrous oxide (Fe2O3·Al2O3·xH2O) adsorbent (inorganic adsorbent) were prepared for this purpose. The sorption behavior of each adsorbent including sorption kinetics, isotherms, effect of pH and selective sorption were investigated in detail. Sorption experimental results showed that the MIP adsorbents had better selectivity for bromate, even in the presence of high concentrations of nitrate, as compared to the inorganic adsorbent. It was found that pH does not affect the adsorption of bromate when using the inorganic adsorbent. Additionally, both adsorbents were immobilized in a polymeric cryogel inside plastic carriers to make them more practical for using in larger scale. Regeneration of the cryogels either containing MIP or inorganic adsorbents were carried out by 0.1 M NaOH and 0.1 M NaCl, respectively. It was found that the regenerated MIP and inorganic adsorbents could be used at least three and five times, respectively, without any loss in their sorption capacity.
Extraction of lutetium(III) from aqueous solutions by employing a single fibre‐supported liquid membrane
Authors: Trtić‐Petrović Tatjana M. , Kumrić Ksenija R. , Đorđević Jelena S. , Vladisavljević Goran T. 
Published: May 2010

AbstractTransport behaviour of Lu(III) across a polypropylene hollow fibre‐supported liquid membrane containing di(2‐ethylhexyl)phosphoric acid (DEHPA) in dihexyl ether as a carrier has been studied. The donor phase was LuCl3 in the buffer solution consisting of 0.2 M sodium acetate at pH 2.5–5.0. A miniaturised system with a single hollow fibre has been operated in a batch mode. The concentration of Lu(III) was determined by indirect voltammetric method using Zn–EDTA complex. The effect of pH and volume of the donor phase, DEHPA concentration in the organic (liquid membrane) phase, the time of extraction and the content of the acceptor phase on the Lu(III) extraction and stripping behaviour was investigated. The results were discussed in terms of the pertraction and removal efficiency, the memory effect and the mean flux of Lu(III). The optimal conditions for the removal of 177Lu(III) from labelled 177Lu‐radiopharmaceuticals were discussed and identified. The removal efficiency of Lu(III) greater than 99% was achieved at pH of the donor phase between 3.5 and 5.0 using DEHPA concentration in the organic phase of 0.47 M and the ratio of the donor to the acceptor phase of 182.
Estimation of global structural and transport properties of peptides through the modeling of their CZE mobility data
Authors: Piaggio Maria V. , Peirotti Marta B. , Deiber Julio A. 
Published: May 2010

AbstractPeptide electrophoretic mobility data are interpreted through a physicochemical CZE model, providing estimates of the equivalent hydrodynamic radius, hydration, effective and total charge numbers, actual ionizing pK, pH‐near molecule and electrical permittivity of peptide domain, among other basic properties. In this study, they are used to estimate some peptide global structural properties proposed, providing thus a distinction among different peptides. Therefore, the solvent drag on the peptide is obtained through a characteristic friction power coefficient of the number of amino acid residues, defined from the global chain conformation in solution. As modeling of the effective electrophoretic mobility of peptides is carried out in terms of particle hydrodynamic size and shape coupled to hydration and effective charge, a packing dimension related to chain conformation within the peptide domain may be defined. In addition, the effective and total charge number fractions of peptides provide some clues on the interpretation of chain conformations within the framework of scaling laws. Furthermore, the model estimates transport properties, such as sedimentation, friction and diffusion coefficients. As the relative numbers of ionizing, polar and non‐polar amino acid residues vary in peptides, their global structural properties defined here change appreciably. Needs for further research are also discussed.
Protein separation using free‐flow electrophoresis microchip etched in a single step
Authors: Wang Pingli , Zhang Lihua , Shan Yichu , Cong Yongzheng , Liang Yu , Han Bin , Liang Zhen , Zhang Yukui 
Published: May 2010

AbstractA one‐step etching method was developed to fabricate glass free‐flow electrophoresis microchips with a rectangle separation microchamber (42 mm‐long, 23 mm‐wide and 28 μm‐deep), in which two glass bridges (0.5 mm‐wide) were made simultaneously to prevent bubbles formed by electrolysis near the Pt electrode from entering the separation chamber. By microchip free‐flow zone electrophoresis, with 200 V voltage applied, the baseline separation of three FITC labeled proteins, ribonuclease B, myoglobin and β‐lactoglobulin, was achieved, with resolution over 1.78. Furthermore, with 2.5 mM Na2SO4 added into the electrode buffer to form higher electrical field strength across separation microchamber than electrode compartments, similar resolution of samples was achieved with the applied voltage decreased to 75 V, which could obviously decrease Joule heat during continuous separation. All these results demonstrate that the free‐flow electrophoresis microchip fabricated by one‐step etching method is suitable for the continuous separation of proteins, which might become an effective pre‐fractionation method for proteome study.
Preferential association between childhood emotional abuse and bipolar disorder
Authors: Etain Bruno , Mathieu Flavie , Henry Chantal , Raust Aurélie , Roy Isabelle , Germain Anne , Leboyer Marion , Bellivier Frank 
Published: May 2010

AbstractChildhood trauma has been suggested to be involved in the susceptibility to bipolar disorder. However, case‐control studies are lacking, and the preferential implication and the dose‐effect of different trauma subtypes remain poorly investigated. Two hundred six bipolar patients and 94 controls completed the Childhood Trauma Questionnaire (CTQ; Bernstein et al., 1994). The CTQ total score was higher for bipolar patients than for controls. The presence of multiple trauma was significantly more frequent in bipolar patients than in controls (63% vs. 33%). Multiple logistic regression suggested that only emotional abuse was associated with bipolar disorder with a suggestive dose‐effect. Clinical practice should include systematic assessment of childhood trauma among bipolar patients with a particular focus on emotional abuse.
Biocompatibility Assessment of Insulating Silicone Polymer Coatings Using an in vitro Glial Scar Assay
Authors: Achyuta Anil Kumar H. , Polikov Vadim S. , White Aleksandr J. , Lewis Hilton G. Pryce , Murthy Shashi K. 
Published: May 2010

AbstractVapor‐deposited silicone coatings are attractive candidates for providing insulation in neuroprosthetic devices owing to their excellent resistivity, adhesion, chemical inertness and flexibility. A biocompatibility assessment of these coatings is an essential part of the materials design process, but current techniques are limited to rudimentary cell viability assays or animal muscle implantation tests. This article describes how a recently developed in vitro model of glial scar formation can be utilized to assess the biocompatibility of vapor‐deposited silicone coatings on micron‐scale wires. A multi‐cellular monolayer comprising mixed glial cells was obtained by culturing primary rat midbrain cells on poly(D‐lysine)‐coated well plates. Stainless steel microwires were coated with two novel insulating thin film silicone polymers, namely poly(trivinyltrimethylcyclotrisiloxane) (polyV3D3) and poly(trivinyltrimethylcyclotrisiloxane–hexavinyldisiloxane) (polyV3D3–HVDS) by initiated chemical vapor deposition (iCVD). The monolayer of midbrain cells was disrupted by placing segments of coated microwires into the culture followed by immunocytochemical analysis after 7 d of implantation. Microglial proximity to the microwires was observed to correlate with the amount of fibronectin adsorbed on the coating surface; polyV3D3–HVDS adsorbed the least amount of fibronectin compared to both stainless steel and polyV3D3. Consequently, the relative number of microglia within 100 µm of the microwires was least on polyV3D3–HVDS coatings compared to steel and polyV3D3. In addition, the astrocyte reactivity on polyV3D3–HVDS coatings was lower compared to stainless steel and polyV3D3. The polyV3D3–HVDS coating was therefore deemed to be most biocompatible, least reactive and most preferable insulating coating for neural prosthetic devices.magnified image
Characterization of Ultra‐Thin Temperature‐Responsive Polymer Layer and Its Polymer Thickness Dependency on Cell Attachment/Detachment Properties
Authors: Fukumori Kazuhiro , Akiyama Yoshikatsu , Kumashiro Yoshikazu , Kobayashi Jun , Yamato Masayuki , Sakai Kiyotaka , Okano Teruo 
Published: May 2010

AbstractUltra thin poly(N‐isopropylacrylamide) (PIPAAm) modified glass coverslips (PIAPAm‐CS) using electron beam irradiation exhibited a clear relationship between the polymer thickness and thermal cell adhesion/detachment behavior. The polymer thickness dependency and the characteristic of ultra thin PIPAAm layer, has been illustrated in terms of the molecular motion of the modified PIPAAm chains. PIPAAm‐CSs surfaces with various area‐polymer densities and thicknesses were characterized by AFM and protein adsorption assay. The newly obtained results gave a further insight into the illustration. Finally, the future application of intelligent surfaces was discussed for fabricating tissue and organ.magnified image
Dimensional upper bounds for admissible subgroups for the metaplectic representation
Authors: Cordero E. , De Mari F. , Nowak K. , Tabacco A. 
Published: May 2010

AbstractWe prove dimensional upper bounds for admissible Lie subgroups H of G = ℍd ⋊ Sp (d, ℝ), d ≥ 2. The notion of admissibility captures natural geometric phenomena of the phase space and it is a sufficient condition for a subgroup to be reproducing. It is expressed in terms of absolutely convergent integrals of Wigner distributions, translated by the affine action of the subgroup. We show that dim H ≤ d2 + 2d, whereas if H ⊂ Sp (d, R), then dim H ≤ d2 + 1. Both bounds are shown to be optimal (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Linking the proteins—Elucidation of proteome‐scale networks using mass spectrometry
Authors: Pflieger Delphine , Gonnet Florence , de la Fuente van Bentem Sergio , Hirt Heribert , de la Fuente Alberto 
Published: May 2010

AbstractProteomes are intricate. Typically, thousands of proteins interact through physical association and post‐translational modifications (PTMs) to give rise to the emergent functions of cells. Understanding these functions requires one to study proteomes as “systems” rather than collections of individual protein molecules. The abstraction of the interacting proteome to “protein networks” has recently gained much attention, as networks are effective representations, that lose specific molecular details, but provide the ability to see the proteome as a whole. Mostly two aspects of the proteome have been represented by network models: proteome‐wide physical protein–protein‐binding interactions organized into Protein Interaction Networks (PINs), and proteome‐wide PTM relations organized into Protein Signaling Networks (PSNs). Mass spectrometry (MS) techniques have been shown to be essential to reveal both of these aspects on a proteome‐wide scale. Techniques such as affinity purification followed by MS have been used to elucidate protein–protein interactions, and MS‐based quantitative phosphoproteomics is critical to understand the structure and dynamics of signaling through the proteome. We here review the current state‐of‐the‐art MS‐based analytical pipelines for the purpose to characterize proteome‐scale networks. © 2010 Wiley Periodicals, Inc., Mass Spec Rev 30:268–297, 2011
The Rosetta Stone of isotope science and the uranium/lead system
Authors: De Laeter John 
Published: May 2010

AbstractThe nucleosynthetic characteristics of U and Pb, together with the interconnectivity between these elements by two radioactive decay chains, are the foundation on which the U/Pb system was able to make a unique contribution to isotope science. The Rosetta Stone is an ancient Egyptian tablet that enabled previously indecipherable hieroglyphics to be translated. In a similar manner, the isotopic investigation of the U/Pb system, by a variety of mass spectrometric instrumentation, has led to our knowledge of the age of the Earth and contributed to thermochronology. In a similar manner, climate change information has been garnered by utilizing the U‐Disequilibrium Series to measure the ages of marine archives. The impact of Pb in the environment has been demonstrated in human health, particularly at the peak of leaded petrol consumption in motor vehicles in the 1970s. Variations in the isotopic composition of lead in samples enable the source of the lead to be “fingerprinted” so as to trace the history of the Pb in ice cores and aerosols. The discovery of nuclear fission of 235U led to the development of nuclear reactors and the isotopic investigation of the Oklo natural reactors. The mass spectrometer is the modern Rosetta Stone of isotope science, which has enabled the isotopic hieroglyphics of the U/Pb system to be investigated to reveal new horizons in our understanding of nature, and to address a number of societal and environmental problems. © 2010 Wiley Periodicals, Inc., Mass Spec Rev 30:757–771, 2011
Structure determination of selaginellins G and H from Selaginella pulvinata by NMR spectroscopy
Authors: Cao Yuan , Chen Ji‐Jun , Tan Ning‐Hua , Wu Yong‐Ping , Yang Jie , Wang Qiang 
Published: May 2010

AbstractSelaginellins G (1) and H (2), two new selaginellin derivatives, were isolated from the whole plant of Selaginella pulvinata. Their structures were elucidated, and complete assignments of the 1H and 13C NMR spectroscopic data were achieved by 1D and 2D NMR experiments (HSQC, HMBC, COSY and ROESY). Compound 1 displayed good antifungal activity against Candida albicans with an IC50 value of 5.3 µg/ml. Copyright © 2010 John Wiley & Sons, Ltd.
Consequences of T2 relaxation during half‐pulse slice selection for ultrashort TE imaging
Authors: Robson Matthew D. , Gatehouse Peter D. 
Published: May 2010

AbstractA “half‐pulse” slice selection approach is used in the ultrashort echo time pulse sequence and is required to give minimal transverse relaxation in a two‐dimensional acquisition. This method splits the normal excitation radiofrequency pulse in half and acquires a pair of images, each using one of these half‐pulses. These half‐pulses are used without a refocusing gradient since summing the pair of images yields images with accurate slice selection. When the radiofrequency pulse duration is similar to the sample T2, characteristics such as the effective echo time and choice of radiofrequency pulse require careful evaluation as some of the approximations in conventional slice selection do not apply. We derive a theory that includes relaxation during excitation using Pauly's excitation k‐space formalism. Further, this theory is tested on phantoms with a range of values of T2 demonstrating the effect on the slice profile. We conclude that relaxation during excitation is significant and should be included in our estimate of the T2 weighting of the sequence. In general, the T2 weighting should be measured from the time of the centroid of the excitation pulse. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.
Patient‐adaptive reconstruction and acquisition in dynamic imaging with sensitivity encoding (PARADISE)
Authors: Sharif Behzad , Derbyshire J. Andrew , Faranesh Anthony Z. , Bresler Yoram 
Published: May 2010

AbstractMRI of the human heart without explicit cardiac synchronization promises to extend the applicability of cardiac MR to a larger patient population and potentially expand its diagnostic capabilities. However, conventional nongated imaging techniques typically suffer from low image quality or inadequate spatio‐temporal resolution and fidelity. Patient‐Adaptive Reconstruction and Acquisition in Dynamic Imaging with Sensitivity Encoding (PARADISE) is a highly accelerated nongated dynamic imaging method that enables artifact‐free imaging with high spatio‐temporal resolutions by utilizing novel computational techniques to optimize the imaging process. In addition to using parallel imaging, the method gains acceleration from a physiologically driven spatio‐temporal support model; hence, it is doubly accelerated. The support model is patient adaptive, i.e., its geometry depends on dynamics of the imaged slice, e.g., subject's heart rate and heart location within the slice. The proposed method is also doubly adaptive as it adapts both the acquisition and reconstruction schemes. Based on the theory of time‐sequential sampling, the proposed framework explicitly accounts for speed limitations of gradient encoding and provides performance guarantees on achievable image quality. The presented in‐vivo results demonstrate the effectiveness and feasibility of the PARADISE method for high‐resolution nongated cardiac MRI during short breath‐hold. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.
A technique for rapid single‐echo spin‐echo T2 mapping
Authors: Sussman Marshall S. , Vidarsson Logi , Pauly John M. , Cheng Hai‐Ling Margaret 
Published: May 2010

AbstractA rapid technique for mapping of T2 relaxation times is presented. The method is based on the conventional single‐echo spin echo approach but uses a much shorter pulse repetition time to accelerate data acquisition. The premise of the new method is the use of a constant difference between the echo time and pulse repetition time, which removes the conventional and restrictive requirement of pulse repetition time ≫ T1. Theoretical and simulation investigations were performed to evaluate the criteria for accurate T2 measurements. Measured T2s were shown to be within 1% error as long as the key criterion of pulse repetition time/T2 ≥3 is met. Strictly, a second condition of echo time/T1 ≪ 1 is also required. However, violations of this condition were found to have minimal impact in most clinical scenarios. Validation was conducted in phantoms and in vivo T2 mapping of healthy cartilage and brain. The proposed method offers all the advantages of single‐echo spin echo imaging (e.g., immunity to stimulated echo effects, robustness to static field inhomogeneity, flexibility in the number and choice of echo times) in a considerably reduced amount of time and is readily implemented on any clinical scanner. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.
Erythrocyte shape reversion from echinocytes to discocytes: Kinetics via fast‐measurement NMR diffusion‐diffraction
Authors: Pages Guilhem , Yau Tsz W. , Kuchel Philip W. 
Published: May 2010

AbstractPulsed field‐gradient spin‐echo (PGSE) NMR spectroscopy via q‐space plots can characterize erythrocyte shapes and their evolution. The present study employed PGSE NMR to investigate shape reversion from advanced echinocytic to normal discocytic shapes due to depletion and then readdition of Mg2+. In q‐space plots of the data, the diffusion‐diffraction minima disappeared for Mg2+‐depleted erythrocytes and reappeared during the shape recovery process, but with lower definition than for control cells. Shape estimates from PGSE NMR spectra and light microscopy were in excellent agreement after application of a scaling/correction factor. 31P NMR was used to probe the biochemical processes activated in erythrocytes after depletion or addition of Mg2+; it showed the activation of the nonoxidative part of the pentose phosphate pathway. Experimental conditions were optimized to bypass this pathway without any influence on the q‐space plots. The release of choline from phosphatidylcholine in the outer leaflet of the plasma membrane of the cells, observed using 1H spin‐echo NMR, showed a higher rate for shape‐recovered than for control cells. This points to a change in phospholipid asymmetry in the plasma membrane. This variation in asymmetry affected the mean cell shape and hence influenced the average alignment of the erythrocytes with the static magnetic field and so affected the shapes of the q‐space plots. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.