bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cadmium exposure increases susceptibility to testicular autoimmunity in mice
Authors: Ogawa Yuki , Itoh Masahiro , Hirai Shuichi , Suna Shigeru , Naito Munekazu , Qu Ning , Terayama Hayato , Ikeda Ayumi , Miyaso Hidenobu , Matsuno Yoshiharu , Komiyama Masatoshi , Mori Chisato 
Published: January 2012

ABSTRACTCadmium, one of various environmental toxicants, is known to suppress systemic immunity and to injure the testicular capillary endothelia with resultant necrosis of testicular tissues in mice and rats treated with high doses. Recently, it also became evident that cadmium can affect the integrity of the blood–testis barrier (BTB), the endocrine function of Leydig cells, apoptosis of germ cells and systemic immunity, even on treatment with a low dose that does not induce spermatogenic disturbance. Experimental autoimmune orchitis (EAO), i.e., an organ‐specific autoimmunity of the testis, can be induced by repeated immunization with testicular antigens, and its pathology is characterized by lymphocytic inflammation and spermatogenic disturbance. In the present study, we investigated the morphological and functional changes of testes in mice treated with a low dose of cadmium chloride (CdCl2) and also examined its toxicity as to susceptibility to EAO. The results showed that exposure to 3 mg CdCl2 kg−1 body weight did not affect the spermatogenic state. However, the BTB at the tubuli recti and the rete testis, but not the seminiferous tubules, was slightly weakened, and intra‐testicular mRNA expression of interleukin (IL)‐6, tumor necrosis factor‐α and IL‐1β was significantly increased by the CdCl2 treatment. Furthermore, immunization with testicular antigens after the CdCl2 exposure significantly augmented the EAO severity. Therefore, exposure to a low dose of CdCl2 induces no significant disturbance of spermatogenesis, however, it does change the immunological microcircumstances in the testis, resulting in increased susceptibility to testicular autoimmunity. Copyright © 2012 John Wiley & Sons, Ltd.
ABO‐incompatible kidney transplantation using regenerative selective immunoglobulin adsorption
Authors: Teschner Sven , Stippel Dirk , Grunenberg Roland , Beck Bodo , Wahba Roger , Gathof Birgit , Benzing Thomas , Burst Volker 
Published: January 2012

AbstractBackground: ABO‐incompatible (ABOi) kidney transplantation is an established procedure relying on the removal of donor‐specific isoagglutinine antibodies as part of the recipient preconditioning. At present, current protocols using immunoadsorption apply a single‐use selective carbohydrate isoagglutinine adsorber. A regenerative and selective immunoglobulin immunoadsorption could be an alternative but has not been reported for ABOi transplantation. Methods: Eight patients were treated with the commonly used isoagglutinine carbohydrate epitope adsorber and seven with a regenerative polyclonal sheep anti‐immunoglobulin adsorber as part of the preconditioning for ABOi kidney transplantation. An IgG‐isoagglutinine titer of less or equal 1:4 qualified for transplantation. Treatment safety, efficiency, length of desensitization, number of postoperative immunoadsorptions, and allograft outcome were retrospectively compared. Results: With the use of the immunoglobulin adsorber the median initial isoagglutinine IgG titers of 1:64 (range 1:32–1:256) were lowered to the target of 1:4 preoperatively with a mean of 6.2 immunoadsorptions (range 5–11). Mean IgG/IgM titer step reduction per IA was 1.98/1.21 for (range 0–4/0–4) and mean titer step rebound 1.31/0.82 (range 0–4/0–3), respectively. The number of immunoadsorptions and length of desensitization was not different from the use of the specific isoagglutinine adsorbers. After transplantation, no rejection occurred and only one postoperative immunoadsorption was necessary. No adverse events in relation to immunoadsorption were observed. Graft function was comparable to the isoagglutinine adsorber group. Conclusion: These data suggest that ABOi kidney transplantation can be performed safely and effectively with a selective regenerative immunoglobulin immunoadsorber. J. Clin. Apheresis, 2012. © 2012 Wiley Periodicals, Inc.
Comparing the biological characteristics of adipose tissue‐derived stem cells of different persons
Authors: Shu Wang , Shu Yang Ting , Dai Chen Yun , Zhen Qin Zhi 
Published: January 2012

AbstractScientists have found that cell sex is a variable that considerably influences the regeneration abilities of muscle‐derived stem cells' in mice. We try to find out whether the cell sex or cell age (the age of donor) will influence the biological characteristics of human adipose tissue‐derived stem cells (H‐ADSCs). The results indicate that cell sex influences the proliferation, differentiation, paracrine, and anti‐apoptosis abilities of the H‐ADSCs, and cell age may also affect the H‐ADSCs' differentiation and anti‐apoptosis abilities. J. Cell. Biochem. 113: 2020–2026, 2012. © 2012 Wiley Periodicals, Inc.
Tumor suppressor function of RUNX3 in breast cancer
Authors: Chen Lin‐Feng 
Published: January 2012

AbstractEmerging evidence indicates that RUNX3 is a tumor suppressor in breast cancer. RUNX3 is frequently inactivated in human breast cancer cell lines and cancer samples by hemizygous deletion of the Runx3 gene, hypermethylation of the Runx3 promoter, or cytoplasmic sequestration of RUNX3 protein. Inactivation of RUNX3 is associated with the initiation and progression of breast cancer. Female Runx3+/− mice spontaneously develop ductal carcinoma, and overexpression of RUNX3 inhibits the proliferation, tumorigenic potential, and invasiveness of breast cancer cells. This review is intended to summarize these findings and discuss the tumor suppressor function of RUNX3 in breast cancer. J. Cell. Biochem. 113: 1470–1477, 2012. © 2012 Wiley Periodicals, Inc.
Downregulation of ErbB3 by Wnt3a contributes to wnt‐induced osteoblast differentiation in mesenchymal cells
Authors: Jullien Nicolas , Maudinet Adrien , Leloutre Benjamin , Ringe Jochen , Häupl Thomas , Marie Pierre J. 
Published: January 2012

AbstractMesenchymal stem cells (MSC) can differentiate into osteoblasts upon activation of Wnt signaling. Identifying targets of Wnt signaling in MSC may help promote MSC osteoblast differentiation for bone regeneration. In this study, using microarray analysis we found that Wnt3a upregulates neuregulin 1 (NRG‐1) during Wnt3a‐induced osteoblast differentiation in primary human MSC and murine C3H10T1/2 mesenchymal cells. Western blot and qPCR analyses confirmed that NRG‐1 is upregulated by Wnt3a, and that this effect was counterbalanced by decreased expression of the NRG‐1 receptor ErbB3. Consistently, exogenous NRG‐1 had no effect on alkaline phosphatase (ALP) activity, an early marker of osteoblast differentiation. In contrast, small interfering RNA‐mediated silencing of endogenous NRG‐1 increased basal and Wnt3a‐induced ALP activity in MSC. We showed that short hairpin (sh) ErbB3 and Wnt3a additively increased β‐catenin transcriptional activity and ALP activity in MSC. These effects were abrogated by DKK1, indicating that cross‐talk between Wnt3a and ErbB3 control MSC osteoblast differentiation via Wnt/β‐catenin signaling. Furthermore, ErbB3 silencing decreased Src expression. Pharmacological inhibition of Src signaling promoted ErbB3‐ and Wnt‐induced ALP activity, suggestive of a role of Src signaling in the modulation of osteoblast differentiation by ErbB3 and Wnt3a. The results indicate that downregulation of ErbB3 induced by Wnt3a contributes to Wnt3a‐induced early osteoblast differentiation of MSCs through increased canonical Wnt/β‐catenin signaling and decreased Src signaling. J. Cell. Biochem. 113: 2047–2056, 2012. © 2012 Wiley Periodicals, Inc.
Direct Electrochemistry of Glucose Oxidase Immobilized at a Novel Composite Material β‐Cyclodextrin/poly(4‐aminothiophenol)/Au Nanoparticle Modified Electrode
Authors: Sheng Qing‐Lin , Luo Kai , Liu Rui‐Xiao , Zheng Jian‐Bin 
Published: January 2012

AbstractA novel complex material was fabricated by three steps. In the first step, gold nanoparticle (Aunano) was prepared with the method of chemistry and dialysis. In the second step, 4‐aminothiophenol (AT) was encapsulated in the cavity of β‐cyclodextrin and formed inclusion complex, cyclodextrin/4‐aminothiophenol (CD/AT). And then inclusion complex was adsorbed to the surface of Aunano based on the bond of Au‐S interaction. In the last step, a complex material, cyclodextrin/poly(4‐aminothiophenol)‐Au nanoparticles (CD/PAT‐Aunano) was obtained by the polymerizing in the acid solution initiated by chlorauric acid. The CD/PAT‐Aunano has spherical nanostructure with the average diameter of 55 nm. Glucose oxidase (GOx) was anchored with this complex material and direct electrochemistry of GOx was achieved. A couple of stable and well‐defined redox peaks were observed with the formal potential (E0′) of ‐0.488 V (vs. SCE) in a pH 6.98 buffer solution. The GOx modified electrode also exhibited an excellent electrocatalytic activity to the reduction of glucose, a linearity range for determination of glucose is from 0.25 mM to 16.0 mM with a detection limit of 0.09 mM (S/N = 3). This protocol had potential application to fabricate the third‐generation biosensor.
Paraptosis accompanied by autophagy and apoptosis was induced by celastrol, a natural compound with influence on proteasome, ER stress and Hsp90
Authors: Wang Wen‐Bo , Feng Li‐Xing , Yue Qing‐Xi , Wu Wan‐Ying , Guan Shu‐Hong , Jiang Bao‐Hong , Yang Min , Liu Xuan , Guo De‐An 
Published: January 2012

AbstractIn the present study, we found that celastrol, a natural compound with well‐known apoptosis‐inducing effect, could also induce paraptosis‐like cytoplasmic vacuolization in cancer cell lines including HeLa cells, A549 cells and PC‐3 cells derived from cervix, lung and prostate, respectively. Further study using HeLa cells indicated that the vacuoles induced by celastrol might be derived from dilation of endoplasmic reticulum. And, in celastrol‐treated cells, markers of autophagy such as transformation of microtubule‐associated protein 1 light chain 3 (LC3)I to LC3II and LC3 punctates formation were identified. Interestingly, autophagy inhibitors could not interrupt but enhance the induction of cytoplasmic vacuolization. Furthermore, MAPK pathways were activated by celastrol and inhibitors of MEK and p38 pathways could prevent the formation of cytoplasmic vacuolization. Celastrol treatment also induced G2/M cell cycle arrest and apoptosis in HeLa cells. In conclusion, celastrol induced a kind of paraptosis accompanied by autophagy and apoptosis in cancer cells. The coincidence of apoptosis and autophagy together with paraptosis might contribute to the unique characteristics of paraptosis in celastrol‐treated cells such as the dependence of paraptosis on MAPK pathways and dynamic change of LC3 proteins. Both paraptosis and apoptosis could contribute to the cell death induced by celastrol while autophagy might serve as a kind of survival mechanism. The potency of celastrol to induce paraptosis, apoptosis and autophagy at the same dose might be related to its capability to affect a variety of pathways including proteasome, ER stress and Hsp90. J. Cell. Physiol. 227: 2196–2206, 2012. © 2011 Wiley Periodicals, Inc.
Intensification of 1‐phenylethanol production by periodical membrane extraction of the product from fermentation broth
Authors: Mihal' Mário , Markoš Jozef , Annus Július , Štefuca Vladimír 
Published: January 2012

AbstractBACKGROUND: Enantioselective bioreduction of acetophenone to S‐(−)‐1‐phenylethanol by Saccharomyces cerevisiae under non‐growth conditions is inhibited by the product created. This study investigated the possibility of intensification and mathematical simulation of 1‐phenylethanol production using periodic product removal carried out by membrane extraction in a hollow fiber membrane module.RESULTS: The highest reaction rate was observed at the beginning of the biotransformation. With increased product concentration in the reaction medium, the reaction rate gradually decreased by about 50% after 20 h of biotransformation. The low concentration of product maintained in the reaction medium using membrane extraction had positive influence on the 1‐phenylethanol production with a high yield (96%) and mean reaction rate of 0.226 mg h−1g−1, 35% higher than biotransformation without product removal. The equilibrium change and membrane fouling caused by biomass were not significant. It was possible to mathematically simulate the whole course of the extractive biotransformation with good agreement with experimental data.CONCLUSION: Bioreductive production of 1‐phenylethanol is more effective when using periodic membrane extraction of the product from the fermentation broth, which gives higher reaction rate, higher yield and simpler downstream process than biotransformation without product removal. Copyright © 2012 Society of Chemical Industry
Oncologic applications of diffusion‐weighted MRI in the body
Authors: Bonekamp Susanne , Corona‐Villalobos Celia P. , Kamel Ihab R. 
Published: January 2012

AbstractDiffusion‐weighted MRI (DWI) allows the detection of malignancies in the abdomen and pelvis. Lesion detection and characterization using DWI largely depends on the increased cellularity of solid or cystic lesions compared with the surrounding tissue. This increased cellularity leads results in restricted diffusion as indicated by reduction in the apparent diffusion coefficient (ADC). Low pretreatment ADC values of several malignancies have been shown to be predictive of better outcome. DWI can assess response to systemic or regional treatment of cancer at a cellular level and will therefore detect successful treatment earlier than anatomical measures. In this review, we provide a brief technical overview of DWI, discuss quantitative image analysis approaches, and review studies which have used DWI for the purpose of detection and characterization of malignancies as well as the early prediction of treatment response. J. Magn. Reson. Imaging 2012;257–279. © 2011 Wiley Periodicals, Inc.
Real‐time magnetic resonance imaging of normal swallowing
Authors: Zhang Shuo , Olthoff Arno , Frahm Jens 
Published: January 2012

AbstractPurpose:To evaluate the use of a novel real‐time magnetic resonance imaging (MRI) technique for the assessment of normal swallowing dynamics.Materials and Methods:In a cohort of 10 healthy subjects, real‐time MRI movies at 24.3 frames per second were obtained in sagittal, coronal, and axial orientation during self‐controlled swallows of 5 mL pineapple juice as oral contrast bolus. All studies were performed with the use of a commercial MRI system at 3 T combining two sets of radiofrequency receiver coils. Real‐time movies relied on a fast low‐angle shot (FLASH) MRI sequence with radial undersampling and image reconstruction by nonlinear inversion yielding 41.23 msec acquisition time for an in‐plane resolution of 1.5 mm. Evaluations focused on clinical image quality as well as visualization and temporal quantification of distinct swallowing functions.Results:Throughout the entire process, the swallowing dynamics were well depicted and characterized with almost no visible image artifacts in all subjects. The mid‐sagittal plane turned out to be most valuable. The movies allowed for a quantitative determination of the temporal pattern of all swallowing events.Conclusion:The proposed real‐time MRI technique yields noninvasive, robust, and quantitative access to the physiology of normal swallowing in healthy subjects at high temporal resolution and image quality. J. Magn. Reson. Imaging 2012;35:1372–1379. © 2012 Wiley Periodicals, Inc.
B2A peptide induces chondrogenic differentiation in vitro and enhances cartilage repair in rats
Authors: Lin Xinhua , Shanmugasundaram Shobana , Liu Yi , Derrien Alexandrine , Nurminskaya Maria , Zamora Paul O. 
Published: January 2012

AbstractThis study investigated whether the synthetic peptide B2A (B2A2‐K‐NS) could induce in vitro chondrogenic differentiation and enhance the in vivo repair of damaged cartilage in an osteoarthritis model. In vitro, micromass cultures of murine and human stem cells with and without B2A were used as models of chondrogenic differentiation. Micromasses were evaluated for gene expression using microarray analysis and quantitative PCR; and for extracellular matrix production by Alcian blue staining for sulfated glycosaminoglycan and immunochemical detection of collagen type II. In vivo, osteoarthritis was chemically induced in knees of adult rats by an injection of mono‐iodoacetate (MIA) into the synovial space. Treatment was administered at 7‐ and 14 days after the MIA by injection into the synovial space of B2A or saline and terminated at 21 days, after which knee cartilage damage was determined and scored by histological analysis. In murine C3H10T1/2 micromass culture, B2A induced the expression of more than 11 genes associated with growth factors/receptors, transcription, and the extracellular matrix, including PDGF‐AA. B2A also significantly increased the sulfated glycosaminoglycan and collagen of murine‐ and human micromass cultures. In the knee osteoarthritis model, B2A treatment enhanced cartilage repair compared to untreated knees as determined histologically by a decrease in damage indicators. These findings suggest that B2A induces stem cells chondrogenic differentiation in vitro and enhances cartilage repair in vivo. The results suggest that B2A might be useful to promote cartilage repair. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:1221–1228, 2012
Variation and pattern in the responses of mammal faunas to Late Pleistocene climatic change in southeastern South Australia
Authors: Macken Amy C. , Prideaux Gavin J. , Reed Elizabeth H. 
Published: January 2012

AbstractWe examined mammal occurrence and variability through the Late Pleistocene vertebrate fossil deposit of Grant Hall in Victoria Fossil Cave, Naracoorte, South Australia. To determine long‐term patterns of change, we compared the composition and relative abundance trends of the assemblage with a nearby Middle Pleistocene deposit in Cathedral Cave. Total species richness did not change through the Grant Hall sequence, dated from 93 ± 8 to 70 ± 5 ka. However, species relative abundances varied between ecologically divergent species, and in some cases between species that demonstrate similar environmental preferences. For some species this variation is comparable to that recorded in Cathedral Cave. Of those showing similar trends between the two deposits, the forest inhabitant, Pseudomys fumeus, recorded an 8.6% decline through Grant Hall, coincident with a 9.7% increase in the dry heath/mallee dweller Pseudomys apodemoides. These patterns indicate that climatic transition from relatively warm, moist to cooler, drier conditions impacted some species in similar ways through climatic cycles of the past. However, the majority of the fauna demonstrated complex responses that are individual and variable through time. Statistical tests of species trends from the Grant Hall assemblage caution that large fossil samples are required to validate patterns observed. Copyright © 2012 John Wiley & Sons, Ltd.
α‐Glucosidase‐ and α‐amylase‐inhibitory activities of phlorotannins from Eisenia bicyclis
Authors: Eom Sung‐Hwan , Lee Sang‐Hoon , Yoon Na‐Young , Jung Won‐Kyo , Jeon You‐Jin , Kim Se‐Kwon , Lee Myung‐Suk , Kim Young‐Mog 
Published: January 2012

AbstractBACKGROUND: In an effort to develop alternative therapeutic agents, strong inhibitory activity against α‐glucosidase and α‐amylase was detected in Eisenia bicyclis methanolic extract.RESULTS: In this study, two phlorotannins were isolated from E. bicyclis and characterised by chromatography and nuclear magnetic resonance. The active substances were identified as fucofuroeckol A (FF) and dioxinodehydroeckol (DD). To the authors' knowledge, this is the first report of the identification of these substances in E. bicyclis. However, to date, no antidiabetic activity of FF and DD has been reported. Both phlorotannins demonstrated significant inhibitory activity against α‐glucosidase and α‐amylase. FF showed potent antidiabetic activity, with IC50 values of 131.34 nmol L−1 against α‐glucosidase and 42.91 µmol L−1 against α‐amylase. The corresponding IC50 values of DD were 93.33 nmol L−1 and 472.7 µmol L−1. Furthermore, kinetic analysis revealed that FF and DD exhibited non‐competitive inhibitory activity against α‐glucosidase.CONCLUSION: These results suggest that FF and DD may be candidates for the development of an antidiabetic pharmaceutical agent or food additive. Copyright © 2012 Society of Chemical Industry
Using modified soy protein to enhance foaming of egg white protein
Authors: Wang Guang , Troendle Molly , Reitmeier Cheryll A , Wang Tong 
Published: January 2012

AbstractBACKGROUND: It is well known that the foaming properties of egg white protein are significantly reduced when a small amount of yolk is mixed in the white. To improve foaming properties of yolk‐contaminated egg white protein, soy protein isolate (SPI) and egg proteins were modified to make basic proteins, and effects of these modified proteins on egg white foaming were evaluated in a model and an angel cake system.RESULTS: SPI and egg yolk proteins were modified to have an isoelectric point of 10, and sonication was used to increase protein dispersibility after the ethyl esterification reaction. However, only the addition of sonicated and modified SPI (SMSPI) showed improvement of foaming in the 5% egg protein model system with 0.4% yolk addition. SMSPI was then used in making angel food cake to examine whether the cake performance reduction due to yolk contamination of the white would be restored by such alkaline protein. Cake performance was improved when cream of tartar was used together with SMSPI.CONCLUSION: Basic soy protein can be made and used to improve egg white foaming properties and cake performance. Copyright © 2012 Society of Chemical Industry
Radiofrequency ablation combined with systemic chemotherapy in nasopharyngeal carcinoma liver metastases improves response to treatment and survival outcomes
Authors: Jin Ying , Cai Yu‐Chen , Cao Ye , Cai Xiu‐Yu , Tan Yu‐Ting , Shi Yan‐Xia , Jiang Wen‐Qi 
Published: January 2012

AbstractBackgroundSystemic chemotherapy is the major treatment modality for nasopharyngeal carcinoma (NPC) liver metastases. We investigated the effectiveness of radiofrequency ablation (RFA) treatment, which has not been well explored in this disease.MethodsOne‐hundred and thirty‐four cases of NPC with liver metastases treated with chemotherapy, chemotherapy with RFA, or RFA alone were retrospectively analyzed. Patient survival was evaluated by the log‐rank test. Survival was analyses using the Kaplan–Meier method. Cox multivariate analyses of clinicopathological features and different treatment approaches were conducted.ResultsLocal response rates were 58% in the RFA group, 78% in the chemotherapy group and 93% in the chemotherapy with RFA group (P < 0.001). Increased progression‐free survival (PFS) and overall survival (OS) were observed in the chemotherapy with RFA group (P < 0.001). Cox multivariate analysis indicated that the number of liver metastases (1 vs. >1), the dimension of the largest liver metastases (≤3 cm vs. >3 cm), evaluation of treatment (response vs. no response) and disease‐free survival (≤12 months vs. >12 months) were independent prognostic factors.ConclusionsRFA combined with chemotherapy is a promising treatment for NPC metastatic liver disease with improved local response, PFS, and OS compared to current chemotherapy protocols. J. Surg. Oncol. 2012; 106:322–326. © 2012 Wiley Periodicals, Inc.