bluuebunny/crossref_metadata_2025_split huggingface dataset:
Three‐dimensional culture systems for the expansion of pluripotent embryonic stem cells
Authors: Storm Michael P. , Orchard Craig B. , Bone Heather K. , Chaudhuri Julian B. , Welham Melanie J. 
Published: June 2010

AbstractMouse embryonic stem cell (ESC) lines, and more recently human ESC lines, have become valuable tools for studying early mammalian development. Increasing interest in ESCs and their differentiated progeny in drug discovery and as potential therapeutic agents has highlighted the fact that current two‐dimensional (2D) static culturing techniques are inadequate for large‐scale production. The culture of mammalian cells in three‐dimensional (3D) agitated systems has been shown to overcome many of the restrictions of 2D and is therefore likely to be effective for ESC proliferation. Using murine ESCs as our initial model, we investigated the effectiveness of different 3D culture environments for the expansion of pluripotent ESCs. Solohill Collagen, Solohill FACT, and Cultispher‐S microcarriers were employed and used in conjunction with stirred bioreactors. Initial seeding parameters, including cell number and agitation conditions, were found to be critical in promoting attachment to microcarriers and minimizing the size of aggregates formed. While all microcarriers supported the growth of undifferentiated mESCs, Cultispher‐S out‐performed the Solohill microcarriers. When cultured for successive passages on Cultispher‐S microcarriers, mESCs maintained their pluripotency, demonstrated by self‐renewal, expression of pluripotency markers and the ability to undergo multi‐lineage differentiation. When these optimized conditions were applied to unweaned human ESCs, Cultispher‐S microcarriers supported the growth of hESCs that retained expression of pluripotency markers including SSEA4, Tra‐1–60, NANOG, and OCT‐4. Our study highlights the importance of optimization of initial seeding parameters and provides proof‐of‐concept data demonstrating the utility of microcarriers and bioreactors for the expansion of hESCs. Biotechnol. Bioeng. 2010;107:683–695. © 2010 Wiley Periodicals, Inc.
Stoichiometric model and metabolic flux analysis for Leptospirillum ferrooxidans
Authors: Merino M.P. , Andrews B.A. , Asenjo J.A. 
Published: June 2010

AbstractA metabolic model for Leptospirillum ferrooxidans was developed based on the genomic information of an analogous iron oxidizing bacteria and on the pathways of ferrous iron oxidation, nitrogen and CO2 assimilation based on experimental evidence for L. ferrooxidans found in the literature. From this metabolic reconstruction, a stoichiometric model was built, which includes 86 reactions describing the main catabolic and anabolic aspects of its metabolism. The model obtained has 2 degrees of freedom, so two external fluxes were estimated to achieve a determined and observable system. By using the external oxygen consumption rate and the generation flux biomass as input data, a metabolic flux map with a distribution of internal fluxes was obtained. The results obtained were verified with experimental data from the literature, achieving a very good prediction of the metabolic behavior of this bacterium at steady state. Biotechnol. Bioeng. 2010;107:696–706. © 2010 Wiley Periodicals, Inc.
Financial, ecological and managerial impacts of emission trading schemes: the case of Lufthansa
Authors: Vespermann Jan , Wittmer Andreas 
Published: June 2010

AbstractCommencing in 2012, emissions from flights departing from or arriving at airports within the European Union (EU) will be covered under the EU emissions trading scheme (ETS). This research analyzes the financial and ecological impacts of the ETS for the Lufthansa Group using a simulation model built on the now‐fixed system design. The results show that while ecological impacts are modest in the first years after introduction, the ETS will result in much higher emission reductions in the medium and long term. These ecological benefits come at the expense of increased financial impacts. This paper argues that the ETS will have a variety of managerial implications at the company level in fields such as environmental monitoring, financial risk management and marketing. Copyright © 2010 John Wiley & Sons, Ltd and ERP Environment.
Miniaturized fluid array for high‐throughput protein expression
Authors: Khnouf Ruba , Olivero Daniel , Jin Shouguang , Fan Z. Hugh 
Published: June 2010

AbstractWe describe a miniaturized fluid array device for high‐throughput cell‐free protein synthesis (CFPS), aiming to match the throughput and scale of gene discovery. Current practice of using E. coli cells for production of recombinant proteins is difficult and cost‐prohibitive to implement in a high‐throughput format. As more and more new genes are being identified, there is a considerable need to have high‐throughput methods to produce a large number of proteins for studying structures and functions of the corresponding genes. The device consists of 96 units and each unit is for expression of one protein; thus up to 96 proteins can be produced simultaneously. The function of the fluid array was demonstrated by expression of a variety of proteins, with more than two orders of magnitude reduction in reagent consumption compared with a commercially available CFPS instrument. The protein expression yield in the device was up to 87 times higher for β‐glucoronidase than that in a conventional microplate. The concentration of β‐galactosidase expressed in the device was determined at 5.5 μg/μL. The feasibility of using the device for drug screening was demonstrated by measuring the inhibitory effects of mock drug compounds on synthesized β‐lactamase without the need for harvesting proteins, which enabled us to reduce the analysis time from days to hours. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
Control of dissolved oxygen concentration using neural network in a batch bioreactor
Authors: Mete Tufan , Ozkan Gulay , Hapoglu Hale , Alpbaz Mustafa 
Published: June 2010

AbstractArtificial neural networks (ANN) have been utilized for many chemical applications because of their ability to learn system features. This paper presents the use of feedforward neural networks for dynamic modelling and dissolved oxygen (DO) control of a batch yeast fermentation. The ARMAX model of this nonlinear process is also presented. Model verification is tested by using experimental data. Different ANNs are trained using the backpropagation learning algorithm. The resulting ANNs are introduced in a Model Predictive Control (MPC) scheme to test the control performance of the structure. At system, output variable that is DO concentration and adjusting variable that is air flow rate are chosen. The robustness of this control structure is studied in the case of setpoint changes. Results obtained with NN‐MPC is also presented and compared with those obtained with Nonlinear Auto Regressive Moving Average (NARMA‐L2) control strategy. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 20: 619–628, 2012
An educational tool for fundamental DC–DC converter circuits and active power factor correction applications
Authors: Kayisli Korhan , Tuncer Servet , Poyraz Mustafa 
Published: June 2010

AbstractComputer‐aided education has become popular thanks to its flexible and useful structure in classroom environment. In this study, a computer‐based educational tool for DC–DC converters and its active power factor correction (PFC) applications are presented for effective education of these circuits at power electronics courses and reinforcement of the theoretical instruction given at these courses. Firstly, simulations were generated by MATLAB/Simulink toolbox. After then, graphical user interfaces (GUIs) were designed for visual approach to specifying all input and output parameters. The results of the educational tool developed are illustrated with screenshots and graphics. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 21: 113–134, 2013
Unusual Photoinduced Electron Transfer from a Zinc Porphyrin to a Tetrapyridyl Free‐Base Porphyrin in a Noncovalent Multiporphyrin Array
Authors: Ventura Barbara , Flamigni Lucia , Beyler Maryline , Heitz Valérie , Sauvage Jean‐Pierre 
Published: June 2010

AbstractExcitation of the peripheral Zn porphyrin units in a noncovalent five‐porphyrin array, formed by gable‐like zinc(II) bisporphyrins and a central free‐base meso‐tetrakis(4‐pyridyl)porphyrin in a 2:1 ratio, (ZnP2)2⋅ (TPyP), does not lead to a quantitative sensitization of the luminescence of the free‐base porphyrin acceptor, even though there is an effective energy transfer. Time resolution of the luminescence evidences a quenching of TPyP upon sensitization by the peripheral ZnP2. The time evolution of the TPyP fluorescence in the complex can be described by a bi‐exponential fitting with a major component of 180 ps and a minor one of 5 ns, compared to an isolated TPyP lifetime of 9.4 ns. The two quenched lifetimes are shown to be correlated to the presence of 2:1 and 1:1 complexes, respectively. No quenching of TPyP fluorescence occurs in (ZnP2)2⋅(TPyP) at 77 K in a rigid solvent for which only an energy‐transfer process (τ=150±10 ps) from peripheral ZnP2 to the central TPyP is observed. An unusual HOMO–HOMO electron‐transfer reaction from ZnP2 to the excited TPyP units, responsible for the observed phenomena, is detected. The resulting charge‐separated state, (ZnP2)+2⋅(TPyP)− is found to recombine to the ground state with a lifetime of 11 ns.
Polyoxopalladates Encapsulating Yttrium and Lanthanide Ions, [XIIIPdII12(AsPh)8O32]5− (X=Y, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb, Lu)
Authors: Barsukova Maria , Izarova Natalya V. , Biboum Rosa Ngo , Keita Bineta , Nadjo Louis , Ramachandran Vasanth , Dalal Naresh S. , Antonova Nadya S. , Carbó Jorge J. , Poblet Josep M. , Kortz Ulrich 
Published: June 2010

AbstractA series of novel yttrium‐ and lanthanide‐containing heteropolyoxopalladates have been prepared and isolated as hydrated sodium salts, Na5[XIIIPdII12(AsPh)8O32]⋅y H2O (X=Y (1), Pr (2), Nd (3), Sm (4), Eu (5), Gd (6), Tb (7), Dy (8), Ho (9), Er (10), Tm (11), Yb (12), Lu (13); y=15–27). The polyanions [XIIIPdII12(AsPh)8O32]5− consist of a cuboid framework of twelve PdII ions with eight phenylarsonate heterogroups located at the vertices and a central guest ion X. The compounds 1–13 have been prepared in a simple one‐pot self‐assembly reaction of Pd(CH3COO)2, phenylarsonic acid and the respective salt of the element X in 0.5 M aqueous sodium acetate solution (pH 6.9), and characterized in the solid state by single‐crystal X‐ray diffraction, elemental and thermogravimetric (TGA) analyses, and IR spectroscopy. It was demonstrated that small, medium, and also large lanthanide ions can be incorporated in the center of the novel heteropolypalladate [XIIIPdII12(AsPh)8O32]5−. The LnO bond lengths follow the expected trend decreasing from left to right in the lanthanide series. This indicates that the {PdII12O32} shell can adjust to the coordination requirements of the encapsulated guest cation. Compounds 3 and 5 were selected for electrochemical studies. Their cyclic voltammetry in a lithium acetate buffer at pH 5.9 showed a Pd0 deposition process on the glassy carbon electrode surface. Coulometry indicated that all PdII centers were reduced to Pd0. The film was stable and could be taken out of the deposition medium and characterized in pure pH 5.9 buffer. Magnetic susceptibility and EPR measurements were carried out on 5 and 6. The former was confirmed to be diamagnetic and the latter strongly paramagnetic with a S=7/2 ground state. DFT calculations for some of the polyoxometalates have been also performed.
ChemInform Abstract: Kinetics and Products of Nitrotoluene Oxidation with Ozone in Acetic Acid.
Authors: GALSTYAN A. G. , GALSTYAN G. A. , TYUPALO N. F. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Asymmetric Reactions Catalyzed by Chiral Metal Complexes. Part 83. Enantioselective Allylic Substitutions Using Ketene Silyl Acetals Catalyzed by a Palladium—Chiral Amidine Complex.
Authors: SAITOH A. , ACHIWA K. , MORIMOTO T. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Asymmetric Kharasch Reaction: Catalytic Enantioselective Allylic Oxidation of Olefins Using Chiral Pyridine Bis(diphenyloxazoline)‐Copper Complexes and tert‐Butyl Perbenzoate.
Authors: SEKAR G. , DATTAGUPTA A. , SINGH V. K. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Stereoselective Conjugate Addition Reactions of α,β‐Unsaturated tert‐Butyl Esters with Aryllithium Reagents.
Authors: FREY L. F. , TILLYER R. D. , CAILLE A.‐S. , TSCHAEN D. M. , DOLLING U. H. , GRABOWSKI E. J. J. , REIDER P. J. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Microwave‐Promoted Lipase‐Catalyzed Reactions.
Authors: LIN G. , LIN W.‐Y. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Aromatic Nucleophilic Substitution in Perfluoro‐1,3,5,7‐tetrahydrobenzo[1,2‐c:4,5‐c′]difuran.
Authors: PLENKIEWICZ H. , DMOWSKI W. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Simultaneous Coordination and Double Activation Phenomena of Carbonyl and Epoxy Oxygens by Bis‐titanium Reagent as a Bidentate Lewis Acid Catalyst.
Authors: ASAO N. , KII S. , HANAWA H. , MARUOKA K. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Conversion of 2‐Methylthio‐4‐trifluoroacetyl‐5,6‐dihydro‐4H‐1,3,4‐thiadiazines into α,β‐Unsaturated Esters via Carbanion‐Induced Ring Opening and Desulfurization.
Authors: SHIMADA K. , OTAKI A. , YANAKAWA M. , MABUCHI S. , YAMAKADO N. , SHIMOGUCHI T. , TAKIKAWA Y. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Cycloreductions via Alkylpalladium Intermediates: Substrate Dependent Cyclopropanations of Palladium Catalyzed Enediyne Cyclizations.
Authors: OH C. H. , KANG J. H. , RHIM C. Y. , KIM J. H. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Lead Tetraacetate Mediated “One‐Pot” Multistage Transformations on Selected Unsaturated 1,2‐Diols: The Wieland—Miescher Series.
Authors: ARSENIYADIS S. , BRONDI‐ALVES R. , QUILEZ DEL MORAL J. , YASHUNSKY D. V. , POTIER P. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Phototrifluoromethylsulfenylation of Phenylcyclopropane.
Authors: MUNAVALLI S. , ROSSMAN D. I. , ROHRBAUGH D. K. , DURST H. D. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: New Intramolecular α‐Arylation Strategy of Ketones by the Reaction of Silyl Enol Ethers to Photosensitized Electron Transfer Generated Arene Radical Cations: Construction of Benzannulated and Benzospiroannulated Compounds.
Authors: PANDEY G. , KARTHIKEYAN M. , MURUGAN A. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Magnesium Alkylidene Carbenoids: Generation from 1‐Halovinyl Sulfoxides with Grignard Reagents and Studies on Their Property, Mechanism, and Some Synthetic Uses.
Authors: SATOH T. , TAKANO K. , OTA H. , SOMEYA H. , MATSUDA K. , KOYAMA M. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Chemoselective Oxidation of Alcohols to Aldehydes and Ketones by tert‐Butyl Hydroperoxide Catalyzed by a Ruthenium Complex of N,N′,N′′‐Trimethyl‐1,4,7‐triazacyclononane.
Authors: FUNG W.‐H. , YU W.‐Y. , CHE C.‐M. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Convenient Synthesis of Hindered N‐Aryl Substituted Cyclic Amines.
Authors: AKULA M. R. , KABALKA G. W. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Reaction of Halothane with Carbonyl Compounds in the Presence of Lithium Diisopropylamide.
Authors: TAKAGI T. , KANAI K. , OMOTE M. , ANDO A. , KUMADAKI I. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The Dealkylation of Tertiary Aliphatic Amines with Phenyl Chlorothionoformate.
Authors: MILLAN D. S. , PRAGER R. H. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: First Intermolecular Hydroacylation of 1,3‐Dienes with Aldehydes Catalyzed by Ruthenium.
Authors: KONDO T. , HIRAISHI N. , MORISAKI Y. , WADA K. , WATANABE Y. , MITSUDO T. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Selective Acetylation of Primary Alcohols: Acetyl and Formyl Transfer Reactions with Copper(II) Salts.
Authors: IRANPOOR N. , FIROUZABADI H. , ZOLFIGOL M. A. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Stannylmetalation of Acetylenic Acids: A Stereoselective Access to Functionalized α,β‐ and β,γ‐Unsaturated Acids.
Authors: THIBONNET J. , LAUNAY V. , ABARBRI M. , DUCHENE A. , PARRAIN J.‐L. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Lithium Azidohydridodiisobutylaluminate in THF as an Efficient Azide Donor.
Authors: YOUN Y. S. , CHO I. S. , CHUNG B. Y. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Ring Opening of Homochiral Bicyclic Oxazolidinones: Synthesis of Allylglycinol Derivatives.
Authors: KIM S.‐G. , AHN K. H. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and Cycloadditions of 1‐[(tert‐Butyldimethylsilyl)oxy]alkenyl Isocyanates.
Authors: DE TOLLENAERE C. , GHOSEZ L. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.