bluuebunny/crossref_metadata_2025_split huggingface dataset:
Neural Measures of Dynamic Changes in Attentive Tracking Load
Authors: Drew Trafton , Horowitz Todd S. , Wolfe Jeremy M. , Vogel Edward K. 
Published: August 2011

Abstract
In everyday life, we often need to track several objects simultaneously, a task modeled in the laboratory using the multiple-object tracking (MOT) task [Pylyshyn, Z., & Storm, R. W. Tracking multiple independent targets: Evidence for a parallel tracking mechanism. Spatial Vision, 3, 179–197, 1988]. Unlike MOT, however, in life, the set of relevant targets tends to be fluid and change over time. Humans are quite adept at “juggling” targets in and out of the target set [Wolfe, J. M., Place, S. S., & Horowitz, T. S. Multiple object juggling: Changing what is tracked during extended MOT. Psychonomic Bulletin & Review, 14, 344–349, 2007]. Here, we measured the neural underpinnings of this process using electrophysiological methods. Vogel and colleagues [McCollough, A. W., Machizawa, M. G., & Vogel, E. K. Electrophysiological measures of maintaining representations in visual working memory. Cortex, 43, 77–94, 2007; Vogel, E. K., McCollough, A. W., & Machizawa, M. G. Neural measures reveal individual differences in controlling access to working memory. Nature, 438, 500–503, 2005; Vogel, E. K., & Machizawa, M. G. Neural activity predicts individual differences in visual working memory capacity. Nature, 428, 748–751, 2004] have shown that the amplitude of a sustained lateralized negativity, contralateral delay activity (CDA) indexes the number of items held in visual working memory. Drew and Vogel [Drew, T., & Vogel, E. K. Neural measures of individual differences in selecting and tracking multiple moving objects. Journal of Neuroscience, 28, 4183–4191, 2008] showed that the CDA also indexes the number of items being tracking a standard MOT task. In the current study, we set out to determine whether the CDA is a signal that merely represents the number of objects that are attended during a trial or a dynamic signal capable of reflecting on-line changes in tracking load during a single trial. By measuring the response to add or drop cues, we were able to observe dynamic changes in CDA amplitude. The CDA appears to rapidly represent the current number of objects being tracked. In addition, we were able to generate some initial estimates of the time course of this dynamic process.
Reduced Specificity of Hippocampal and Posterior Ventrolateral Prefrontal Activity during Relational Retrieval in Normal Aging
Authors: Giovanello Kelly S. , Schacter Daniel L. 
Published: August 2011

AbstractNeuroimaging studies of episodic memory in young adults demonstrate greater functional neural activity in ventrolateral pFC and hippocampus during retrieval of relational information as compared with item information. We tested the hypothesis that healthy older adults—individuals who exhibit behavioral declines in relational memory—would show reduced specificity of ventrolateral prefrontal and hippocampal regions during relational retrieval. At study, participants viewed two nouns and were instructed to covertly generate a sentence that related the words. At retrieval, fMRIs were acquired during item and relational memory tasks. In the relational task, participants indicated whether the two words were previously seen together. In the item task, participants indicated whether both items of a pair were previously seen. In young adults, left posterior ventrolateral pFC and bilateral hippocampal activity was modulated by the extent to which the retrieval task elicited relational processing. In older adults, activity in these regions was equivalent for item and relational memory conditions, suggesting a reduction in ventrolateral pFC and hippocampal specificity with normal aging.
On the Vertical Decay Rate of the Maximum Tangential Winds in Tropical Cyclones
Authors: Stern Daniel P. , Nolan David S. 
Published: August 2011

Abstract
In this study, it is shown that the maximum tangential winds within tropical cyclones decrease with height at a percentage rate that is nearly independent of both the maximum wind speed and the radius of maximum winds (RMW). This can be seen by normalizing the profiles of maximum tangential winds Vmax by their respective values at 2-km height. From Doppler radar analyses, profiles of maximum normalized tangential wind Vmaxnorm are found to share a common shape, despite spanning a great range of intensities. There is a systematic dependence of Vmaxnorm on intensity and size, but it is shown to be small, and the mean profile of Vmaxnorm can be used to accurately “predict” the individual profiles of Vmax. Using Emanuel’s steady-state analytical vortex model, it is shown that Vmaxnorm is essentially independent of the size of the RMW. It is shown mathematically that the near independence of Vmaxnorm from size is due to the facts that the RMW is nearly a surface of constant absolute angular momentum M and that its outward slope increases linearly with radius. As the slope of the RMW is not a function of intensity, Vmaxnorm is also nearly independent of intensity in theory, and this is confirmed using Emanuel’s simple time-dependent model. In contrast to intensity, it is shown that Vmaxnorm increases with potential intensity. A suite of idealized simulations using the Weather Research and Forecasting model (WRF) are used to further examine the manner in which the maximum winds change with height. Above 2-km height, vertical profiles of Vmaxnorm are nearly independent of both intensity and size. Occasional deviations from this near-universal profile in these simulations are due to unbalanced winds, and it is proposed that this is the cause of occasional observations of maximum winds that are nearly constant with height through the midtroposphere, as in Hurricane Gloria (1985) and Hurricane Dennis (2005).
A Systematic Relationship between Intraseasonal Variability and Mean State Bias in AGCM Simulations
Authors: Kim Daehyun , Sobel Adam H. , Maloney Eric D. , Frierson Dargan M. W. , Kang In-Sik 
Published: August 2011

Abstract
Systematic relationships between aspects of intraseasonal variability (ISV) and mean state bias are shown in a number of atmospheric general circulation model (AGCM) simulations. When AGCMs are categorized as either strong ISV or weak ISV models, it is shown that seasonal mean precipitation patterns are similar among models in the same group but are significantly different from those of the other group. Strong ISV models simulate excessive rainfall over the South Asian summer monsoon and the northwestern Pacific monsoon regions during boreal summer. Larger ISV amplitude also corresponds closely to a larger ratio of eastward-to-westward-propagating variance, but no model matches the observations in both quantities simultaneously; a realistic eastward-to-westward ratio is simulated only when variance exceeds that observed. Three sets of paired simulations, in which only one parameter in the convection scheme is changed to enhance the moisture sensitivity of convection, are used to explore the common differences between the two groups in greater detail. In strong ISV models, the mean and the standard deviation of surface latent heat flux is greater, convective rain fraction is smaller, and tropical tropospheric temperatures are lower compared to weak ISV models. The instantaneous joint relationships between daily gridpoint relative humidity and precipitation differ in some respects when strong and weak ISV models are compared, but these differences are not systematic enough to explain the differences in ISV amplitude. Conversely, there are systematic differences in the frequency with which specific values of humidity and precipitation occur. In strong ISV models, columns with a higher saturation fraction and rain rate occur more frequently and make a greater contribution to total precipitation.
Patellar Tendon Ruptures in National Football League Players
Authors: Boublik Martin , Schlegel Theodore , Koonce Ryan , Genuario James , Lind Charles , Hamming David 
Published: August 2011

Background: Although knee injuries are common among professional football players, ruptures of the patellar tendon are relatively rare. Predisposing factors, mechanisms of injury, treatment guidelines, and recovery expectations are not well established in high-level athletes. Hypothesis: Professional football players with isolated rupture of the patellar tendon treated with timely surgical repair will return to their sport. Study Design: Case series; Level of evidence, 4. Methods: Twenty-four ruptures of the patellar tendon in 22 National Football League (NFL) players were identified from 1994 through 2004. Team physicians retrospectively reviewed training room and clinic records, operative notes, and imaging studies for each of these players. Player game statistics and draft status were analyzed to identify return to play predictors. A successful outcome was defined as participating in 1 regular-season NFL game. Results: Eleven of the 24 injuries had antecedent symptoms. The most common mechanism of injury was an eccentric overload to a contracting extensor mechanism. Physical examination demonstrated a palpable defect in all players. Twenty-two were complete ruptures, and 2 were partial injuries. Three of the 24 cases had a concomitant anterior cruciate ligament (ACL) injury. In 19 of the 24 injuries, the player returned to participate in at least 1 game in the NFL. Players who returned were drafted, on average, in the fourth round, while those who failed to return to play were drafted, on average, in the sixth round. Of those players who returned to play, the average number of games played was 45.4, with a range of 1 to 142 games. Conclusion: Patellar tendon ruptures can occur in otherwise healthy professional football players without antecedent symptoms or predisposing factors. The most common mechanism of injury is eccentric overload. Close attention should be paid to stability examination of the knee given the not uncommon occurrence of concomitant ACL injury. Although this is usually a season-ending injury when it occurs in isolation, acute surgical repair generally produces good functional results and allows for return to play the following season. Players chosen earlier in the draft are more likely to return to play.
Tagging SNPs in REN, AGTR1 and AGTR2 genes and response of renin activity, angiotensin II and aldosterone concentrations to antihypertensive treatment in Kazakans
Authors: Yan Weili , Zhang Yuanming , Shan Zimei , Wang Qian , Huang Yongdi , Wang Chenchen , Yan Kai 
Published: August 2011

Hypothesis: Polymorphisms of REN, AGTR1 and AGTR2 may be associated with responses of renin–angiotensin–aldosterone system (RAAS) activity phenotypes to angiotensin-converting enzyme inhibitor (ACEI) antihypertensive treatment. Materials and methods: A total of 400 first diagnosed Kazak hypertensives were randomly allocated to two groups and received a 3-week course of either captopril and atenolol as monotherapy under double blinding. Genotype–phenotype association analyses were performed by covariance analyses between baseline level and responses of blood pressure, renin, angiotensin II and aldosterone concentrations with tagging single nucleotide polymorphisms (SNPs) in REN, AGTR1 and AGTR2 genes. A false discovery rate method was used to adjust multiple testing. Results: After adjustment for multiple testing, we found that the G allele of rs6676670 (T/G) in intron 1 of REN was significantly associated with higher baseline aldosterone concentrations ( p < 0.0001, explained variance (EV) = 2.3%). Significant associations after adjustments were also found between the A allele of rs2887284, with higher baseline renin activity ( p = 0.022, EV = 1.0%), higher responses of renin ( p = 0.018 EV = 5.4%), and higher responses of angiotensin II ( p = 0.0255, EV = 3.13%) to the treatment of ACEI. The carriers of the A allele of rs2887284 appeared to be more sensitive to the ACEI treatment. Conclusion: rs2887284 in intron 9 of REN is associated with the response of renin and angiotensin II levels to ACEI treatment.
How I treat thalassemia
Authors: Rachmilewitz Eliezer A. , Giardina Patricia J. 
Published: August 2011

Abstract
The purpose of this article is to set forth our approach to diagnosing and managing the thalassemias, including β-thalassemia intermedia and β-thalassemia major. The article begins by briefly describing recent advances in our understanding of the pathophysiology of thalassemia. In the discussion on diagnosing the condition, we cover the development of improved diagnostic tools, including the use of very small fetal DNA samples to detect single point mutations with great reliability for prenatal diagnosis of homozygous thalassemia. In our description of treatment strategies, we focus on how we deal with clinical manifestations and long-term complications using the most effective current treatment methods for β-thalassemia. The discussion of disease management focuses on our use of transfusion therapy and the newly developed oral iron chelators, deferiprone and deferasirox. We also deal with splenectomy and how we manage endocrinopathies and cardiac complications. In addition, we describe our use of hematopoietic stem cell transplantation, which has produced cure rates as high as 97%, and the use of cord blood transplantation. Finally, we briefly touch on therapies that might be effective in the near future, including new fetal hemoglobin inducers and gene therapy.
TLR7 enables cross-presentation by multiple dendritic cell subsets through a type I IFN-dependent pathway
Authors: Oh Jason Z. , Kurche Jonathan S. , Burchill Matthew A. , Kedl Ross M. 
Published: August 2011

AbstractConjugation of TLR agonists to protein or peptide antigens has been demonstrated in many studies to be an effective vaccine formula in inducing cellular immunity. However, the molecular and cellular mediators involved in TLR-induced immune responses have not been carefully examined. In this study, we identify Type I IFN and IL-12 as critical mediators of cross-priming induced by a TLR7 agonist-antigen conjugate. We demonstrate that TLR7-driven cross-priming requires both Type I IFN and IL-12. Signaling through the IFN-αβR was required for the timely recruitment and accumulation of activated dendritic cells in the draining lymph nodes. Although IL-12 was indispensable during cross-priming, it did not regulate DC function. Therefore, the codependency for these 2 cytokines during TLR7-induced cross-priming is the result of their divergent effects on different cell-types. Furthermore, although dermal and CD8α+ DCs were able to cross-prime CD8+ T cells, Langerhans cells were unexpectedly found to potently cross-present antigen and support CD8+ T-cell expansion, both in vitro and in vivo. Collectively, the data show that a TLR7 agonist-antigen conjugate elicits CD8+ T-cell responses by the coordinated recruitment and activation of both tissue-derived and lymphoid organ-resident DC subsets through a Type I IFN and IL-12 codependent mechanism.
Hepcidin expression in human airway epithelial cells is regulated by interferon-γ
Authors: Frazier Marie D , Mamo Lisa B , Ghio Andrew J , Turi Jennifer L 
Published: August 2011

Abstract

Background
Hepcidin serves as a major regulator of systemic iron metabolism and immune function. Airway epithelial cells have an extensive interface with the environment, and so must be able to respond locally to the presence of particulates, infection, and inflammation. Therefore, we hypothesized that hepcidin is expressed in airway epithelial cells and is regulated by early phase cytokines.

Methods
Primary, differentiated human bronchial epithelial (NHBE) cells were used to assess hepcidin gene expression in response to IFN-γ, TNF-α, IL-1β, and IL-6, as well as to LPS + CD14. The role of the Janus Kinase-signal transducer and activator of transcription (JAK-STAT) pathway in IFN-γ-mediated hepcidin production was assessed by measuring JAK2 phophorylation and STAT1 nuclear translocation. Inductively coupled plasma mass spectroscopy (ICP-MS) was used to determine whether hepcidin altered iron transport in either NHBE cells or primary alveolar macrophages.

Results
We demonstrate that differentiated human airway epithelial cells express hepcidin mRNA and that its expression is augmented in response to IFN-γ via activation of STAT1. However, while IFN-γ induced hepcidin gene expression, we were not able to demonstrate diminished expression of the iron export protein, ferroportin (Fpn), at the cell surface, or iron accumulation in airway epithelial in the presence of exogenous hepcidin.

Conclusion
These data demonstrate that airway epithelial cells express hepcidin in the lung in response to IFN-γ. The presence of hepcidin in the airway does not appear to alter cellular iron transport, but may serve as a protective factor via its direct antimicrobial effects.
Genotyping common and rare variation using overlapping pool sequencing
Authors: He Dan , Zaitlen Noah , Pasaniuc Bogdan , Eskin Eleazar , Halperin Eran 
Published: August 2011

Abstract

Background
Recent advances in sequencing technologies set the stage for large, population based studies, in which the ANA or RNA of thousands of individuals will be sequenced. Currently, however, such studies are still infeasible using a straightforward sequencing approach; as a result, recently a few multiplexing schemes have been suggested, in which a small number of ANA pools are sequenced, and the results are then deconvoluted using compressed sensing or similar approaches. These methods, however, are limited to the detection of rare variants.

Results
In this paper we provide a new algorithm for the deconvolution of DNA pools multiplexing schemes. The presented algorithm utilizes a likelihood model and linear programming. The approach allows for the addition of external data, particularly imputation data, resulting in a flexible environment that is suitable for different applications.

Conclusions
Particularly, we demonstrate that both low and high allele frequency SNPs can be accurately genotyped when the DNA pooling scheme is performed in conjunction with microarray genotyping and imputation. Additionally, we demonstrate the use of our framework for the detection of cancer fusion genes from RNA sequences.
Functional characterization of the sciarid BhC4-1 core promoter in transgenic Drosophila
Authors: Garcia Adriana C , Gitaí Daniel LG , Humann Fernanda C , Paçó-Larson Maria L , Monesi Nadia 
Published: August 2011

Abstract

Background
Core promoters are cis-regulatory modules to which bind the basal transcriptional machinery and which participate in the regulation of transcription initiation. Although core promoters have not been extensively investigated through functional assays in a chromosomal context, the available data suggested that the response of a given core promoter might vary depending on the promoter context. Previous studies suggest that a (-57/+40) fragment constitutes the core promoter of the BhC4-1 gene which is located in DNA puff C4 of the sciarid fly Bradysia hygida. Here we tested this (-57/+40) fragment in distinct regulatory contexts in order to verify if promoter context affects its core promoter activity.

Results
Consistent with the activity of a core promoter, we showed that in the absence of upstream regulatory sequences the (-57/+40) fragment drives low levels of reporter gene mRNA expression throughout development in transgenic Drosophila. By assaying the (-57/+40) fragment in two distinct regulatory contexts, either downstream of the previously characterized Fbp1 enhancer or downstream of the UAS element, we showed that the BhC4-1 core promoter drives regulated transcription in both the germline and in various tissues throughout development. Furthermore, the use of the BhC4-1 core promoter in a UAS construct significantly reduced salivary gland ectopic expression in third instar larvae, which was previously described to occur in the context of the GAL4/UAS system.

Conclusions
Our results from functional analysis in transgenic Drosophila show that the BhC4-1 core promoter drives gene expression regardless of the promoter context that was assayed. New insights into the functioning of the GAL4/UAS system in Drosophila were obtained, indicating that the presence of the SV40 sequence in the 3' UTR of a UAS construct does not preclude expression in the germline. Furthermore, our analysis indicated that ectopic salivary gland expression in the GAL4/UAS system does not depend only on sequences present in the GAL4 construct, but can also be affected by the core promoter sequences in the UAS construct. In this context, we propose that the sciarid BhC4-1 core promoter constitutes a valuable core promoter which can be employed in functional assays in insects.
Vitamin D binding protein variants associate with asthma susceptibility in the Chinese han population
Authors: Li Fei , Jiang Lei , Willis-Owen Saffron A , Zhang Youming , Gao Jinming 
Published: August 2011

Abstract

Background
Asthma is a genetically heterogeneous disease. Polymorphisms of genes encoding components of the vitamin D pathway have been reported to associate with the risk of asthma. We have previously demonstrated that vitamin D status was associated with lung function in Chinese asthma patients. In this study, we tested whether polymorphisms of genes encoding for vitamin D receptor (VDR), vitamin D 25-hydroxylase (CYP2R1) and vitamin D binding protein (GC) were associated with asthma in the Chinese Han population.

Methods
We sequenced all 8 exons of VDR and all 5 exons of CYP2R1 in a Chinese case-control cohort of asthma consisting of 467 cases and 288 unrelated healthy controls. Two mutations were identified in these regions. These variants were specified as rs2228570 in exon 2 of VDR and rs12794714 in exon 1 of CYP2R1. We also genotyped two common polymorphisms in GC gene (rs4588 and rs7041) by a PCR-restriction fragment length polymorphism (RFLP) method. We analyzed the association between these 4 polymorphisms and asthma susceptibility and asthma-related traits.

Results
Polymorphic markers in VDR and CYP2R1 were not associated with asthma in the Chinese Han cohort. Importantly, variants in GC gene, which give rise to the two most common electrophoretic isoforms of the vitamin D binding protein, were associated with asthma susceptibility. Compared with isoform Gc1, Gc2 was significantly associated with the risk of asthma (OR = 1.35, 95% CI = 1.01-1.78 p = 0.006).

Conclusions
The results provide supporting evidence for association between GC variants and asthma susceptibility in the Chinese Han population.