bluuebunny/crossref_metadata_2025_split huggingface dataset:
The roles of Hippo/YAP signaling pathway in physical therapy
Authors: Pan Chunran , Hao Xiaoxia , Deng Xiaofeng , Lu Fan , Liu Jiawei , Hou Wenjie , Xu Tao 
Published: April 2024

AbstractCellular behavior is regulated by mechanical signals within the cellular microenvironment. Additionally, changes of temperature, blood flow, and muscle contraction also affect cellular state and the development of diseases. In clinical practice, physical therapy techniques such as ultrasound, vibration, exercise, cold therapy, and hyperthermia are commonly employed to alleviate pain and treat diseases. However, the molecular mechanism about how these physiotherapy methods stimulate local tissues and control gene expression remains unknow. Fortunately, the discovery of YAP filled this gap, which has been reported has the ability to sense and convert a wide variety of mechanical signals into cell-specific programs for transcription, thereby offering a fresh perspective on the mechanisms by which physiotherapy treat different diseases. This review examines the involvement of Hippo/YAP signaling pathway in various diseases and its role in different physical therapy approaches on diseases. Furthermore, we explore the potential therapeutic implications of the Hippo/YAP signaling pathway and address the limitations and controversies surrounding its application in physiotherapy.
Combined effects of blood pressure and glucose status on the risk of chronic kidney disease
Authors: Toyama Maya , Satoh Michihiro , Nakayama Shingo , Hashimoto Hideaki , Muroya Tomoko , Murakami Takahisa , Hirose Takuo , Obara Taku , Nakaya Naoki , Mori Takefumi , Ohkubo Takayoshi , Imai Yutaka , Hozawa Atsushi , Metoki Hirohito 
Published: April 2024

AbstractThis study aimed to assess the combined effects of blood pressure (BP) and glucose status on chronic kidney disease (CKD) incidence in young and middle-aged adults. We examined data from 1,297,341 Japanese individuals aged <60 years (60.1% men; mean age 41.4 ± 9.3 years) with no history of CKD at baseline. The interval-censored Cox proportional hazards model with covariates was used. During a median follow-up period of 2.1 years, new onset CKD (estimated glomerular filtration rate <60 ml/min/1.73 m2 and/or proteinuria) occurred in 80,187 participants. In participants without antihypertensive treatment (AHT), the adjusted hazard ratios (95% confidence interval) per 1-standard deviation, that is, 15 mmHg increase in systolic BP for CKD incidence, were 1.08 (1.07–1.09), 1.12 (1.10–1.13), and 1.15 (1.12–1.18) in normoglycemia, borderline glycemia, and diabetes groups, respectively. These ratios were significantly higher in the borderline glycemia and diabetes groups compared with those in the normoglycemia group (interaction p < 0.0001). The interaction between BP and borderline glycemia was evident when the outcome definition was restricted to proteinuria. In participants under AHT, systolic BP was most strongly associated with CKD risk in the diabetes group, although no significant interaction was observed. High BP and high glucose status may synergistically increase the incidence of CKD. Strict BP management may play an important role in the early prevention of CKD in individuals with worse glucose status within the young and middle-aged population.
NASA GeneLab derived microarray studies of Mus musculus and Homo sapiens organisms in altered gravitational conditions
Authors: Adamopoulos Konstantinos I. , Sanders Lauren M. , Costes Sylvain V. 
Published: April 2024

AbstractOne of the greatest challenges of humanity for deep space exploration is to fully understand how altered gravitational conditions affect human physiology. It is evident that the spaceflight environment causes multiple alterations to musculoskeletal, cardiovascular, immune and central nervous systems, to name a few known effects. To better characterize these biological effects, we compare gene expression datasets from microarray studies found in NASA GeneLab, part of the NASA Open Science Data Repository. In this review, we summarize these archived results for various tissues, emphasizing key genes which are highly reproducible in different mice or human experiments. Such exhaustive mining shows the potential of NASA Open Science data to identify and validate mechanisms taking place when mammalian organisms are exposed to microgravity or other spaceflight conditions. Our comparative meta-analysis findings highlight certain degrees of overlap and reproducibility in genes identified as differentially expressed within musculoskeletal tissues in each species across a variety of altered gravity conditions. However, the level of overlap between species was found to be significantly limited, partly attributed to the limited availability of human samples.
Amplified positive effects on air quality, health, and renewable energy under China’s carbon neutral target
Authors: Qin Yue , Zhou Mi , Hao Yueting , Huang Xin , Tong Dan , Huang Liangdian , Zhang Chuan , Cheng Jing , Gu Weiyi , Wang Licheng , He Xiaojia , Zhou Derong , Chen Qi , Ding Aijun , Zhu Tong 
Published: April 2024

AbstractChina pledged to achieve carbon neutrality by 2060 to combat global climate change, yet the resulting multi-aspect domestic impacts are not fully analysed due to an incomplete understanding of the underlying anthropogenic–natural interactions. Building an integrated cross-disciplinary modelling framework that can capture the feedbacks of changing aerosols on meteorology, here we highlight the amplified air quality, human health and renewable energy self-reinforcing synergies of China’s carbon neutral target in comparison to the baseline in 2015 and 2060. We find that owing to emissions reduction and more favourable meteorological conditions caused by less aerosol, achieving China’s carbon neutrality target in 2060 reduces national population-weighted PM2.5 concentrations and associated premature deaths by ~39 μg m−3 and 1.13 (95% confidence interval: 0.97–1.29) million while boosting provincial solar (wind) power performance by up to ~10% (~6%) with mostly decreased resource variability in comparison to the 2060 baseline. Enhanced renewable performance along with low-carbon energy transition may provide additional opportunities to address the remaining air pollution and associated human health damages upon achieving carbon neutrality. Our results highlight that global developing and polluting countries’ pledge for carbon neutrality can produce important positive feedbacks between aerosols mitigation, air quality improvement and enhanced renewable energy, which can be amplified via weakened aerosol–meteorology interactions and better atmospheric dispersion.
Chromosome-level genome assembly of the pygmy grasshopper Eucriotettix oculatus (Orthoptera: Tetrigoidea)
Authors: Li Ran , Qin Yingcan , Rong Wantao , Deng Wei-an , Li Xiaodong 
Published: April 2024

AbstractThe pygmy grasshoppers, which belong to the superfamily Tetrigoidea, exhibit remarkable environmental adaptability. However, no study has yet reported a reference genome for this group. In this study, we assembled a high-quality chromosome-scale genome of Eucriotettix oculatus, which survive in the environment heavily polluted by heavy metals, achieved through Illumina and PacBio sequencing, alongside chromosome conformation capture techniques. The resulting genome spans 985.45 Mb across seven chromosomes (range: 71.55 to 266.65 Mb) and features an N50 length of 123.82 Mb. Chr5 is considered to be the single sex chromosome (X). This genome is composed of 46.42% repetitive elements and contains 14,906 predicted protein-coding genes, 91.63% of which are functionally annotated. Decoding the E. oculatus genome not only promotes future studies on environmental adaptation for the pygmy grasshopper, but also provides valuable resources for in-depth investigation on phylogeny, evolution, and behavior of Orthoptera.
Comprehensiveness cuproptosis related genes study for prognosis and medication sensitiveness across cancers, and validation in prostate cancer
Authors: Yang Longfei , Tang Yifan , Zhang Yuwei , Wang Yang , Jiang Peng , Liu Fengping , Feng Ninghan 
Published: April 2024

AbstractCuproptosis-related genes (CRGs) are important for tumor development. However, the functions of CRGs across cancers remain obscure. We performed a pan-cancer investigation to reveal the roles of CRGs across cancers. In an analysis of 26 cancers, 12 CRGs were differentially expressed, and those CRGs were found to have prognostic value across different cancer types. The expression of CRGs exhibited varied among tumors of 6 immune subtypes and were significantly correlated with the 16 sensitivities of drugs. The expression of CRGs were highly correlated with immunological subtype and tumor microenvironment (TME) of prostate cancer. We also established CRGs-related prognostic signatures that closely correlated with prognosis and drug sensitivity of prostate cancer patients. Single-cell RNA-seq revealed that several CRGs were enriched in the cancer cells. Finally, an in vitro experiment showed that elesclomol, a cuproptosis inducer, targets ferredoxin 1 and suppress cell viability in prostate cancer cells. In conclusion, we carried out a comprehensive investigation for determining CRGs in differential expression, prognosis, immunological subtype, TME, and cancer treatment sensitivity across 26 malignancies; and validated the results in prostate cancer. Our research improves pan-cancer knowledge of CRGs and identifies more effective immunotherapy strategies.
Evaluating the effects of mefenoxam on taxonomic and functional dynamics of nontarget fungal communities during carrot cultivation
Authors: Tagele Setu Bazie , Gachomo Emma W. 
Published: April 2024

AbstractRidomil Gold SL (45.3% a.i. mefenoxam) is a widely used chemical fungicide for the control of oomycetes. However, its impact on fungal communities remains unexplored. Therefore, the goal of this study was to examine the effects of mefenoxam on the temporal dynamics of fungal taxonomic and functional diversities during carrot cultivation under four treatment groups: mefenoxam application with and without Pythium inoculation, and untreated control groups with and without Pythium inoculation. Our in vitro sensitivity assay showed that the maximum recommended concentration of mefenoxam, 0.24 ppm, did not suppress the mycelial growth of P. irregulare. At 100 ppm, mycelial growth was only reduced by 11.4%, indicating that the isolate was resistant to mefenoxam. MiSeq sequencing data revealed transient taxonomic variations among treatments 2 weeks post-treatment. Mortierella dominated the fungal community in the mefenoxam-Pythium combination treatment, as confirmed through PCR using our newly designed Mortierella-specific primers. Conversely, mefenoxam-Pythium combination had adverse effects on Penicillium, Trichoderma, and Fusarium, and decrease the overall alpha diversity. However, these compositional changes gradually reverted to those observed in the control by the 12th week. The predicted ecological functions of fungal communities in all Pythium and mefenoxam treatments shifted, leading to a decrease in symbiotrophs and plant pathogen functional groups. Moreover, the community-level physiological profiling approach, utilizing 96-well Biolog FF microplates, showed discernible variations in the utilization of 95 diverse carbon sources among the treatments. Notably, arbutin, l-arabinose, Tween 80, and succinamic acid demonstrated a strong positive association with Mortierella. Our findings demonstrate that a single application of mefenoxam at its recommended rate triggers substantial taxonomic and functional shifts in the soil fungal community. Considering this impact, the conventional agricultural practice of repeated mefenoxam application is likely to exert considerable shifts on the soil ecosystem that may affect agricultural sustainability.
Erythromycin mediates co-flocculation between cyanobacterium Synechocystis sp. PCC 6803 and filamentous fungi in liquid cultivation without organic compounds
Authors: Pichaiyotinkul Panutchaya , Leksingto Jidapa , Sukkasam Nannaphat , In-na Pichaya , Incharoensakdi Aran , Monshupanee Tanakarn 
Published: April 2024

AbstractPhotoautotrophic cyanobacteria assimilate the greenhouse gas carbon dioxide as their sole carbon source for producing useful bioproducts. However, harvesting the cells from their liquid media is a major bottleneck in the process. Thus, an easy-to-harvest method, such as auto-flocculation, is desirable. Here, we found that cyanobacterium Synechocystis sp. PCC 6803 co-flocculated with a natural fungal contamination in the presence of the antibiotic erythromycin (EM) but not without EM. The fungi in the co-flocculated biomass were isolated and found to consist of five species with the filamentous Purpureocillium lilacinum and Aspergillus protuberus making up 71% of the overall fungal population. The optimal co-cultivation for flocculation was an initial 5 mg (fresh weight) of fungi, an initial cell density of Synechocystis of 0.2 OD730, 10 µM EM, and 14 days of cultivation in 100 mL of BG11 medium with no organic compound. This yielded 248 ± 28 mg/L of the Synechocystis-fungi flocculated biomass from 560 ± 35 mg/L of total biomass, a 44 ± 2% biomass flocculation efficiency. Furthermore, the EM treated Synechocystis cells in the Synechocystis-fungi flocculate had a normal cell color and morphology, while those in the axenic suspension exhibited strong chlorosis. Thus, the occurrence of the Synechocystis-fungi flocculation was mediated by EM, and the co-flocculation with the fungi protected Synechocystis against the development of chlorosis. Transcriptomic analysis suggested that the EM-mediated co-flocculation was a result of down-regulation of the minor pilin genes and up-regulation of several genes including the chaperone gene for pilin regulation, the S-layer protein genes, the exopolysaccharide-polymerization gene, and the genes for signaling proteins involved in cell attachment and abiotic-stress responses. The CuSO4 stress can also mediate Synechocystis-fungi flocculation but at a lower flocculation efficiency than that caused by EM. The EM treatment may be applied in the co-culture between other cyanobacteria and fungi to mediate cell bio-flocculation.
Simple risk scoring using sinus rhythm electrocardiograms predicts the incidence of atrial fibrillation in the general population
Authors: Kamada Hiroyuki , Kawasoe Shin , Kubozono Takuro , Ninomiya Yuichi , Enokizono Kei , Yoshimoto Issei , Iriki Yasuhisa , Ikeda Yoshiyuki , Miyata Masaaki , Miyahara Hironori , Tokushige Koichi , Ohishi Mitsuru 
Published: April 2024

AbstractAtrial fibrillation (AF) is an arrhythmic disease. Prediction of AF development in healthy individuals is important before serious complications occur. We aimed to develop a risk prediction score for future AF using participants’ data, including electrocardiogram (ECG) measurements and information such as age and sex. We included 88,907 Japanese participants, aged 30–69 years, who were randomly assigned to derivation and validation cohorts in a ratio of 1:1. We performed multivariate logistic regression analysis and obtained the standardised beta coefficient of relevant factors and assigned scores to them. We created a score based on prognostic factors for AF to predict its occurrence after five years and applied it to validation cohorts to assess its reproducibility. The risk score ranged from 0 to 17, consisting of age, sex, PR prolongation, QT corrected for heart rate prolongation, left ventricular hypertrophy, premature atrial contraction, and left axis deviation. The area under the curve was 0.75 for the derivation cohort and 0.73 for the validation cohort. The incidence of new-onset AF reached over 2% at 10 points of the risk score in both cohorts. Thus, in this study, we showed the possibility of predicting new-onset AF using ECG findings and simple information.
A study of correlations between cephalometric measurements in Koreans with normal occlusion by network analysis
Authors: Jeong Seorin , Kim Sehyun , Lim Sung-Hoon , Yu Sun-Kyoung 
Published: April 2024

AbstractAnalyzing the correlation between cephalometric measurements is important for improving our understanding of the anatomy in the oral and maxillofacial region. To minimize bias resulting from the design of the input data and to establish a reference for malocclusion research, the aims of this study were to construct the input set by integrating nine cephalometric analyses and to study the correlation structure of cephalometric variables in Korean adults with normal occlusion. To analyze the complex correlation structure among 65 cephalometric variables, which were based on nine classical cephalometric analyses, network analysis was applied to data obtained from 735 adults (368 males, 367 females) aged 18–25 years with normal occlusion. The structure was better revealed through weighted network analysis and minimum spanning tree. Network analysis revealed cephalometric variable clusters and the inter- and intra-correlation structure. Some metrics were divided based on their geometric interpretation rather than their clinical significance. It was confirmed that various classical cephalometric analyses primarily focus on investigating nine anatomical features. Investigating the correlation between cephalometric variables through network analysis can significantly enhance our understanding of the anatomical characteristics in the oral and maxillofacial region, which is a crucial step in studying malocclusion using artificial intelligence.
Synergistic catalytic mechanism of red mud in the co-gasification of spirit-based distillers’ grains and sewage sludge
Authors: Zeng Xi , Wang Junliang , Yang Aijiang , Cao Yang 
Published: April 2024

AbstractExperiments of co-gasification of spirit-based distillers’ grains (SDG) and sewage sludge (SS) were carried out with red mud (RM) by using a self-designed fixed-bed gasifier. The effects of RM addition, gasification reaction temperature, SS and SDG blending ratio and other factors on the gasification reaction characteristics and synergism were investigated. The results are as follow: RM had catalytic effect on SS and SDG co-gasification, which can enhance the gasification reaction and H2 yield; increasing the temperature can enhance the gasification reaction and reduce the syngas H2/CO; with the increase of SDG, the H2 yield gradually grew; with the rise of SS, the gasification reaction gradually augmented. The catalytic mechanism was mainly due to the redox cycle of Fe2O3 in RM, which can promote the water transfer reaction. At the same time, the eutectic mixture of K, Na, Ca, Fe and other metal elements at high temperatures was the main reason for the synergistic effect.
Association between being breastfed in infancy and adult colorectal cancer risk among Japanese men and women
Authors: Minami Yuko , Kanemura Seiki , Kusaka Jun , Kinouchi Makoto , Suzuki Shinichi , Iwasashi Hajime , Nishino Yoshikazu , Kakugawa Yoichiro , Miura Koh 
Published: April 2024

AbstractIt has been postulated that being breastfed in infancy affects not only health status in childhood but also disease risk in adulthood. To investigate the association of being breastfed with the risks of adult colorectal cancer and benign tumor, we conducted a case–control study including 1190 colorectal cancer and 1585 benign tumor cases and 5301 controls, admitted to a single hospital in Miyagi Prefecture, Japan, between 1997 and 2013. History of having been breastfed was assessed using a self-administered questionnaire, and odds ratios (ORs) were estimated using unconditional logistic regression. There was no association between being breastfed and colorectal cancer risk (breastfed versus formula-only fed, OR = 1.21; 95% CI 0.87–1.67). There was also no association with the risk of benign tumor (OR = 1.04). On the other hand, analyses stratified by sex and birth year found heterogeneous associations. Women born after 1950 who had been breastfed tended to have increased risks of colorectal cancer (OR = 1.58) and benign tumor (OR = 1.51) relative to those who had been formula-only fed, although not statistically significant. In men born after 1950, being breastfed was associated with a significantly decreased risk of benign tumor (OR = 0.57; 95% CI 0.33–0.98).
Computationally guided synthesis of a hierarchical [4[2+3]+6] porous organic ‘cage of cages’
Authors: Zhu Qiang , Qu Hang , Avci Gokay , Hafizi Roohollah , Zhao Chengxi , Day Graeme M. , Jelfs Kim E. , Little Marc A. , Cooper Andrew I. 
Published: April 2024

AbstractHere we report a two-step, hierarchical synthesis that assembles a trigonal prismatic organic cage into a more symmetric, higher-order tetrahedral cage, or ‘cage of cages’. Both the preformed [2+3] trigonal prismatic cage building blocks and the resultant tetrahedral [4[2+3]+6]cage molecule are constructed using ether bridges. This strategy affords the [4[2+3]+6]cage molecule excellent hydrolytic stability that is not a feature of more common dynamic cage linkers, such as imines. Despite its relatively high molar mass (3,001 g mol−1), [4[2+3]+6]cage exhibits good solubility and crystallizes into a porous superstructure with a surface area of 1,056 m2 g−1. By contrast, the [2+3] building block is not porous. The [4[2+3]+6]cage molecule shows high CO2 and SF6 uptakes due to its polar skeleton. The preference for the [4[2+3]+6]cage molecule over other cage products can be predicted by computational modelling, as can its porous crystal packing, suggesting a broader design strategy for the hierarchical assembly of organic cages with synthetically engineered functions.
Functional and structural insights into activation of TRPV2 by weak acids
Authors: Haug Ferdinand M , Pumroy Ruth A , Sridhar Akshay , Pantke Sebastian , Dimek Florian , Fricke Tabea C , Hage Axel , Herzog Christine , Echtermeyer Frank G , de la Roche Jeanne , Koh Adrian , Kotecha Abhay , Howard Rebecca J , Lindahl Erik , Moiseenkova-Bell Vera , Leffler Andreas 
Published: April 2024

AbstractTransient receptor potential (TRP) ion channels are involved in the surveillance or regulation of the acid-base balance. Here, we demonstrate that weak carbonic acids, including acetic acid, lactic acid, and CO2 activate and sensitize TRPV2 through a mechanism requiring permeation through the cell membrane. TRPV2 channels in cell-free inside-out patches maintain weak acid-sensitivity, but protons applied on either side of the membrane do not induce channel activation or sensitization. The involvement of proton modulation sites for weak acid-sensitivity was supported by the identification of titratable extracellular (Glu495, Glu561) and intracellular (His521) residues on a cryo-EM structure of rat TRPV2 (rTRPV2) treated with acetic acid. Molecular dynamics simulations as well as patch clamp experiments on mutant rTRPV2 constructs confirmed that these residues are critical for weak acid-sensitivity. We also demonstrate that the pore residue Glu609 dictates an inhibition of weak acid-induced currents by extracellular calcium. Finally, TRPV2-expression in HEK293 cells is associated with an increased weak acid-induced cytotoxicity. Together, our data provide new insights into weak acids as endogenous modulators of TRPV2.