bluuebunny/crossref_metadata_2025_split huggingface dataset:
Assessment of right ventricle volumes and function by cardiac MRI: Quantification of the regional and global interobserver variability
Authors: Bonnemains Laurent , Mandry Damien , Marie Pierre‐Yves , Micard Emilien , Chen Bailiang , Vuissoz Pierre‐André 
Published: September 2011

AbstractReproducibility of the manual assessment of right ventricle volumes by short‐axis cine‐MRI remains low and is often attributed to the difficulty in separating the right atrium from the ventricle. This study was designed to evaluate the regional interobserver variability of the right ventricle volume assessment to identify segmentation zones with the highest interobserver variability. Short‐axis views of 90 right ventricles (30 hypertrophic, 30 dilated, and 30 normal) were acquired with 2D steady‐state free precession sequences at 1.5 T and were manually segmented by two observers. The two segmentations were compared and the variations were quantified with a variation score based on the Hausdorff distance between the two segmentations and the interobserver 95% limits of concordance of the global volumes. The right ventricles were semiautomatically split into four subregions: apex, mid‐ventricle, tricuspid zone, and infundibulum. These four subregions represented 11%, 34%, 36%, and 19% of the volume but, respectively, yielded variation scores of 8%, 16%, 42%, and 34%. The infundibulum yielded the highest interobserver regional variability although its variation score remained comparable to the tricuspid zone due to its lower volume. These results emphasize the importance of standardizing the segmentation of the infundibulum and the tricuspid zone to improve reproducibility. Magn Reson Med, 2011. © 2011 Wiley‐Liss, Inc.
Measurement of liver iron overload: Noninvasive calibration of MRI‐R2* by magnetic iron detector susceptometer
Authors: Gianesin B. , Zefiro D. , Musso M. , Rosa A. , Bruzzone C. , Balocco M. , Carrara P. , Bacigalupo L. , Banderali S. , Rollandi G. A. , Gambaro M. , Marinelli M. , Forni G. L. 
Published: September 2011

AbstractAn accurate assessment of body iron accumulation is essential for the diagnosis and therapy of iron overload in diseases such as thalassemia or hemochromatosis. Magnetic iron detector susceptometry and MRI are noninvasive techniques capable of detecting iron overload in the liver. Although the transverse relaxation rate measured by MRI can be correlated with the presence of iron, a calibration step is needed to obtain the liver iron concentration. Magnetic iron detector provides an evaluation of the iron overload in the whole liver. In this article, we describe a retrospective observational study comparing magnetic iron detector and MRI examinations performed on the same group of 97 patients with transfusional or congenital iron overload. A biopsy‐free linear calibration to convert the average transverse relaxation rate in iron overload (R2 = 0.72), or in liver iron concentration evaluated in wet tissue (R2 = 0.68), is presented. This article also compares liver iron concentrations calculated in dry tissue using MRI and the existing biopsy calibration with liver iron concentrations evaluated in wet tissue by magnetic iron detector to obtain an estimate of the wet‐to‐dry conversion factor of 6.7 ± 0.8 (95% confidence level). Magn Reson Med, 2011. © 2011 Wiley‐Liss, Inc.
Necrotizing myopathy: Clinicoserologic associations
Authors: Ellis Elizabeth , Ann Tan Ju , Lester Sue , Tucker Graeme , Blumbergs Peter , Roberts‐Thomson Peter , Limaye Vidya 
Published: September 2011

AbstractIntroduction: Necrotizing myopathy (NM) is distinguished from idiopathic inflammatory myositis (IIM) by dominance of myofiber necrosis, lack of mononuclear inflammatory infiltrates, and presence of antibodies to signal recognition particle (SRP). Methods: The clinical features of 64 cases of NM were determined. Measurement of autoantibodies was undertaken on stored sera from 23 patients with NM. The incidence of malignancy was determined from the South Australian Cancer Registry. Results: NM patients showed male predominance (61%), more frequent myalgias, and higher creatine kinase (CK) levels compared with IIM patients. Patients with NM had a high incidence of systemic lupus erythematosus (SLE) (21%), hypertension (11 of 17, 65%), and diabetes mellitus (3 of 13, 23%). No patient had antibodies to SRP. NM patients showed no altered risk for malignancy compared with the South Australian population (P = 0.86). Conclusions: NM is associated with SLE, hypertension, and diabetes mellitus. Comprehensive assessment of cardiovascular risk is indicated in NM, which raises the possibility of targeted interventions. Muscle Nerve, 2012
p38γ activity is required for maintenance of slow skeletal muscle size
Authors: Foster William H. , Tidball James G. , Wang Yibin 
Published: September 2011

AbstractIntroduction:p38γ kinase is highly enriched in skeletal muscle and is implicated in myotube formation. However, the activation status of p38γ in muscle is unclear.Methods:p38γ activity in slow and fast adult mouse skeletal muscle tissue was examined, as was the impact of p38γ deficiency on muscle development and gene expression.Results:p38γ is preferentially activated in slow muscle, but it is inactive in fast muscle types. Furthermore, the loss of p38γ in mice led to decreased muscle mass associated with a smaller myofiber diameter in slow muscle, but there was no impact on fast muscle in either mass or myofiber diameter. Finally, p38γ‐deficient muscle showed selective changes in genes related to muscle growth in slow muscle fibers.Conclusion:This study provides evidence that p38γ is selectively activated in slow skeletal muscle and is involved in the normal growth and development of a subset of skeletal muscle. Muscle Nerve, 2012
A reconstruction algorithm for electrical impedance tomography based on sparsity regularization
Authors: Jin Bangti , Khan Taufiquar , Maass Peter 
Published: September 2011

SUMMARYThis paper develops a novel sparse reconstruction algorithm for the electrical impedance tomography problem of determining a conductivity parameter from boundary measurements. The sparsity of the ‘inhomogeneity’ with respect to a certain basis is a priori assumed. The proposed approach is motivated by a Tikhonov functional incorporating a sparsity‐promoting ℓ1‐penalty term, and it allows us to obtain quantitative results when the assumption is valid. A novel iterative algorithm of soft shrinkage type was proposed. Numerical results for several two‐dimensional problems with both single and multiple convex and nonconvex inclusions were presented to illustrate the features of the proposed algorithm and were compared with one conventional approach based on smoothness regularization. Copyright © 2011 John Wiley & Sons, Ltd.
Distinguishing Bearded Vulture Activities within Archaeological Contexts: Identification Guidelines
Authors: Marín‐Arroyo A. B. , Margalida A. 
Published: September 2011

ABSTRACTIn Europe, the bearded vultureGypaetus barbatusis currently an endangered species limited now to high mountain areas, but had a broader geographical distribution in the past. It breeds on ledges in limestone cliffs, in habitats similar to those also selected by prehistoric human groups. This species feeds mainly on bones of medium‐sized ungulates that are processed before ingestion at bone‐breaking sites or ossuaries; bone remains subsequently accumulate at their nesting places leading to potential mixing with human‐derived or carnivore‐derived assemblages. This fact could lead to incorrect palaeoeconomic interpretations that can be avoided if the taphonomic contribution of this bird of prey is correctly identified. Here, we present some key features to distinguish its presence in archaeo/palaeontological contexts. Bone surface alterations, breakage patterns and skeletal profiles are recorded. Several prehistoric, historic and modern assemblages accumulated by bearded vultures are studied. In addition, a new utility index based on bearded vulture dietary preferences that can be compared with skeletal element abundance in terms of %MAU (Minimal Animal Units) has been defined. Copyright © 2011 John Wiley & Sons, Ltd.
Prediction of the risk for gastric cancer using candidate methylation markers in the non‐neoplastic gastric mucosae
Authors: Shin Cheol Min , Kim Nayoung , Park Ji Hyun , Kang Gyeong Hoon , Kim Joo Sung , Jung Hyun Chae , Song In Sung 
Published: September 2011

AbstractAberrant DNA methylation is frequently found during gastric carcinogenesis. Recently, we identified potential methylation markers important for Helicobacter pylori‐induced gastric carcinogenesis using an Illumina methylation chip assay. In this study, we evaluated the candidate genes as markers for gastric cancer (GC) in a large Korean population. DNA methylation of PTPN6, MOS, DCC, CRK, and VAV1 was evaluated in non‐neoplastic gastric specimens using quantitative methylation‐specific PCR in patients with GC (n = 207) and their age‐ and gender‐matched controls (n = 207). Methylation levels in 125 GC samples were also compared. H. pylori infection status was categorized as negative, active, or past infection according to the results of endoscopy‐based tests (CLOtest, histology, and culture), H. pylori serology, and serum pepsinogen test. In the controls, active H. pylori infection increased methylation levels in DCC, CRK, MOS, and VAV1 but decreased methylation levels in PTPN6 (all p < 0.05); the methylation levels in MOS remained increased in patients with past H. pylori infection compared to H. pylori‐negative subjects (p < 0.001). Methylation levels in MOS in non‐neoplastic gastric mucosae increased in the presence of GC, regardless of H. pylori infection status (p < 0.01). Methylation levels in all genes but DCC decreased significantly in GC specimens compared to neoplastic gastric mucosae (p < 0.01); however, methylation levels in GC tissues were not correlated with those in their background gastric mucosae. Hypomethylation of MOS in GC tissues was associated with tumour invasion, nodal metastasis, and undifferentiated histology (p < 0.05). To summarize, among the candidate genes, DNA methylation of MOS may reflect the duration of H. pylori exposure and may be a marker for the development of GC. Copyright © 2012 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
1H and 13C nuclear magnetic resonance assignments of polyneuridine, 19‐(Z)‐akuammidine and 16‐epi‐voacarpine
Authors: Lin Long‐Ze , Cordell Geoffrey A. 
Published: September 2011

AbstractThe proton and carbon‐13 nuclear magnetic resonance (NMR) spectra of polyneuridine from Rhazya orientalis, 19‐(Z)‐akuammidine and 16‐epi‐voacarpine from Gelsemium elegans have been unambiguously assigned through COSY, nOe, AFT, HETCOR, CSCM 1D and selective INEPT spectroscopy.
HPLC Analysis of the main alkaloids from Eschscholtzia Californica cham
Authors: Guédon D. , Cappelaere N. , Simanek V. 
Published: September 2011

AbstractA rapid prechromatographic sample preparation and a reversed phase high performance liquid chromatographic (RP‐HPLC) ion pair method have been developed for the qualitative and quantitative analysis of the main alkaloids of Eschscholtzia californica, namely allocryptopine, californidine, chelerythrine, eschscholtzine, N‐methyllaurotetanine, protopine and sanguinarine. The identity and purity of the component HPLC‐peaks were established by photodiode array detection and by comparison with standards. The distribution of alkaloids in the organs of the plant was examined: allocryptopine is the main constituent in roots, whilst californidine is the major alkaloid in all aerial organs except flowers where the main compound is eschscholtzine. HPLC fingerprint chromatograms of extracts of E. californica from different commercial sources were found to be similar.
Novel bioresorbable hydrogels prepared from chitosan‐graft‐polylactide copolymers
Authors: Hu Yanfei , Liu Yufei , Qi Xu , Liu Pei , Fan Zhongyong , Li Suming 
Published: September 2011

AbstractA series of biodegradable chitosan‐graft‐polylactide (CS‐g‐PLA) copolymers were prepared by grafting of poly(L‐lactide) (PLLA) or poly(D‐lactide) (PDLA) precursor to the backbone of chitosan using N,N′‐carbonyldiimidazole as coupling agent. The composition of the copolymers was varied by adjusting the chain length of PLA as well as the ratio of chitosan to PLA. The copolymers synthesized via this ‘graft‐onto’ method present interesting properties as shown by NMR and infrared spectroscopy, gel permeation chromatography and solubility tests. Hydrogels were prepared by mixing water‐soluble CS‐g‐PLLA and CS‐g‐PDLA solutions. Gelation was assigned to stereocomplexation between PLLA and PDLA blocks as evidenced by differential scanning calorimetry and wide‐angle X‐ray diffraction measurements. Thymopentin (TP5) was taken as a model drug to evaluate the potential of these CS‐g‐PLA hydrogels as drug carriers. An initial burst and a final release up to 82% of TP5 were observed from high‐performance liquid chromatography analysis. Copyright © 2011 Society of Chemical Industry
Proteomic analysis of brain proteins in APP/PS‐1 human double mutant knock‐in mice with increasing amyloid β‐peptide deposition: Insights into the effects of in vivo treatment with N‐acetylcysteine as a potential therapeutic intervention in mild cognitive impairment and Alzheimer's disease
Authors: Robinson Renã A. S. , Joshi Gururaj , Huang Quanzhen , Sultana Rukhsana , Baker Austin S. , Cai Jian , Pierce William , St. Clair Daret K. , Markesbery William R. , Butterfield D. Allan 
Published: September 2011

AbstractProteomics analyses were performed on the brains of wild‐type (WT) controls and an Alzheimer's disease (AD) mouse model, APP/PS‐1 human double mutant knock‐in mice. Mice were given either drinking water or water supplemented with N‐acetylcysteine (NAC) (2 mg/kg body weight) for a period of five months. The time periods of treatment correspond to ages prior to Aβ deposition (i.e. 4–9 months), resembling human mild cognitive impairment (MCI), and after Aβ deposition (i.e. 7–12 months), more closely resembling advancing stages of AD. Substantial differences exist between the proteomes of WT and APP/PS‐1 mice at 9 or 12 months, indicating that Aβ deposition and oxidative stress lead to downstream changes in protein expression. Altered proteins are involved in energy‐related pathways, excitotoxicity, cell cycle signaling, synaptic abnormalities, and cellular defense and structure. Overall, the proteomic results support the notion that NAC may be beneficial for increasing cellular stress responses in WT mice and for influencing the levels of energy‐ and mitochondria‐related proteins in APP/PS‐1 mice.
One‐pot nonreductive O‐glycan release and labeling with 1‐phenyl‐3‐methyl‐5‐pyrazolone followed by ESI‐MS analysis
Authors: Wang Chengjian , Fan Wancui , Zhang Ping , Wang Zhongfu , Huang Linjuan 
Published: September 2011

AbstractA novel one‐pot procedure for the nonreductive release of O‐linked glycans from glycoproteins and the simultaneous derivatization of released glycans with 1‐phenyl‐3‐methyl‐5‐pyrazolone (PMP) is described. Unlike the traditional reductive β‐elimination, which produces alditols, this new method employs PMP/ammonia aqueous solution as the reaction medium. The O‐glycans are released from glycoproteins and derivatized with PMP nonreductively, specifically, and quantitatively. Samples can be easily purified from ammonia, excess PMP, and peptide residues by evaporation, chloroform extraction, and solid‐phase extraction (SPE) column fractionation for HPLC, CE, or MS analysis. The procedure has been elaborated with two purified glycoproteins, porcine stomach mucin and bovine fetuin, and successfully applied to O‐glycan profiling of a challenging biological specimen, healthy human plasma. This new procedure has shown methodological significance in O‐glycan analysis.
Cell response of Escherichia coli to cisplatin‐induced stress
Authors: Stefanopoulou Maria , Kokoschka Malte , Sheldrick William S. , Wolters Dirk A. 
Published: September 2011

AbstractCisplatin is undoubtedly one of the most common and successful anticancer drugs worldwide. Though its DNA‐based mechanism of action is well established, the contribution of the proteome to this process remains unclear. The possible impact of particular Escherichia coli proteins on the cytostatic activity of cisplatin was the subject of this study. Our main focus was not only the ”bottom‐up” identification of novel cisplatin protein targets through LC/LC‐MS/MS analysis, but also a label‐free quantification of their regulation profile by spectral‐counting. The regulation of two proteins, aconitate hydratase 2 and 60 kDa chaperonin 1, could be linked to a platinated amino acid in the protein sequence, whereas in the cases of 30S ribosomal protein S1 and enolase, it could be shown that cisplatin fragments are coordinated to an essential site for the functionality of the protein. Nucleoside triphosphate pyrophosphohydrolase (MazG) regulates the programmed cell death and was found to be platinated on the protein surface, which probably correlates with the established mode of action. A possible new chapter in the understanding of cisplatin's mechanism of action and its severe side effects is opened, since evidence is provided that platinated proteins are not only involved in cellular stress response but also in energy metabolism through glycolysis and catabolic processes, in gene regulatory mechanisms and protein synthesis.
Influence of polymer particle size on the percolation threshold of electrically conductive latex‐based composites
Authors: Moriarty Gregory P. , Whittemore James H. , Sun Katherine Ann , Rawlins James W. , Grunlan Jaime C. 
Published: September 2011

AbstractMonodispersed copolymer emulsions, each with a different polymer particle size, were used to investigate the effect of particle size on the electrical and thermomechanical properties of carbon black (CB)‐filled segregated network composites. These emulsions were synthesized with equal moles of methyl methacrylate and butyl acrylate, with latex particle size ranging from 83 to 771 nm. The electrical percolation threshold was found to decrease from 2.7 to 1.1 vol % CB as the latex particle size was increased. Microstructural images reveal diminished latex coalescence, and improved CB segregation, with increasing latex particle size. In general, coalescence is shown to increase for all systems with increasing CB concentration. Furthermore, all systems exhibited a similar maximum electrical conductivity plateau of 0.7 S cm−1, albeit at lower concentration for larger latex particle size. This ability to tailor percolation threshold with latex particle size provides an important tool for manipulating electrical and mechanical properties of polymer nanocomposites. © 2011 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 49: 1547–1554, 2011
Dispersion of multiwalled carbon nanotubes in a rubber matrix using an internal mixer: Effects on rheological and electrical properties
Authors: Charman Maxime , Léonardi Frédéric , Dominguez Sébastien , Bissuel Cédric , Derail Christophe 
Published: September 2011

AbstractThe dispersion of multiwalled carbon nanotubes (CNTs) in an ethylene propylene rubber matrix was investigated using an internal mixer. Poly(ethylene‐co‐polyvinyl acetate) (EVA) statistic copolymer was used as a dispersing agent. The effects of the concentration of the dispersing agent and the matrix viscosity on the quality of the dispersion of 1 wt % of CNTs were studied by using microscopy and rheology in the melt state. It was demonstrated that the dispersion is governed principally by the viscosity of the matrix. As expected, better dispersion was observed when the matrix exhibited a lower viscosity. The influence of the filler content on the rheological and electrical properties is presented. A Cross model with a yield stress is proposed to describe the rheological behavior of these materials, which exhibit a viscoelastic solid behavior from 1 wt % CNT content. Electrical measurement data indicate that the electrical percolation threshold was 2.9 wt %. © 2011 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys 49: 1597–1604, 2011
Differences in depressed oncologic patients' narratives after receiving two different therapeutic interventions for depression: a qualitative study
Authors: Rodríguez Vega Beatriz , Orgaz Barnier Pablo , Bayón Carmen , Palao Ángela , Torres Guadalupe , Hospital Ana , Benito Guillermo , Dieguez Maria , Fernández Liria Alberto 
Published: September 2011

AbstractBackgroundThis study aims to explore differences in personal narratives of the experience of illness and treatment in depressed oncologic patients who received either combined treatment for depression (psychotherapy plus antidepressants) or standard treatment (antidepressants alone).MethodsWe employed a qualitative research design based on grounded theory. Data were collected from eight videotaped focus groups and semi‐structured interviews with a total of 28 participants. The research team reviewed interview transcripts and categorized the participants' responses using the ATLAS.ti (ATLAS.ti Scientific Software Development GmbH Hardenbergstr. 7 D‐10623, Berlin) software package.ResultsCompared with patients in the standard treatment group, patients in the combined treatment group were better able to relate their experiences of physical and emotional discomfort and find meaning in the experience of illness by viewing cancer as a transformative experience. In addition, patients in the combined treatment group tended to use more active coping strategies based on acceptance of their situation and emphasized that psychotherapy had been helpful.ConclusionsQualitative analysis is an efficient method of examining the meaning of quantitative results in depth, particularly patients' perspectives on quality of life. Patients undergoing combined treatment consider psychotherapy to be a helpful tool and exhibit more personal growth than do patients undergoing standard treatment. Copyright © 2011 John Wiley & Sons, Ltd.
The influence of knowledge and perception of the risk of cervical cancer on screening behavior in mainland Chinese women
Authors: Gu Can , Chan Carmen W. H. , Twinn Sheila , Choi Kai Chow 
Published: September 2011

AbstractBackgroundTheories of health behavior and empirical research highlight the risk perception as a significant factor for people adopting cancer screening. However, screening uptakes and risk perception of cervical cancer in mainland Chinese women remains unknown.ObjectiveThis paper adopted the protection motivation theory (PMT) to examine Chinese women's knowledge and perceptions of cervical cancer risk and factors influencing utilization of cervical screening.MethodsA self‐administered questionnaire was completed by 167 participants in mainland China (79 nonscreened and 88 screened women) in 2007 which consisted of four sections: background information, women's attendance pattern for cervical screening, perceptions related to body health and knowledge about cervical cancer and screening, and PMT measures.ResultsAll women considered themselves at low risk of cervical cancer. No significant association was observed between previous screening uptake and PMT variables. Using multivariate analysis, having children, a perception that visiting doctors regularly is important to health, average and high levels of knowledge about cervical screening were significantly associated with having been received screening.ConclusionChinese women demonstrated an unrealistic optimism about their personal risk of cervical cancer. The findings do not support an association between risk perception and screening uptake. In spite of this, current findings revealed some possible factors influencing women's screening behavior. This study highlights the significance of knowledge and culturally‐relevant health behavior and beliefs about cervical screening for Chinese women in determining whether or not they receive screening. The promotion of cervical cancer prevention and early detection should be integrated into public education about women's health. Copyright © 2011 John Wiley & Sons, Ltd.