bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bevacizumab in association with de Gramont 5‐fluorouracil/folinic acid in patients with oxaliplatin‐, irinotecan‐, and cetuximab‐refractory colorectal cancer
Authors: Vincenzi Bruno , Santini Daniele , Russo Antonio , Spoto Chiara , Venditti Olga , Gasparro Simona , Rizzo Sergio , Zobel Bruno Beomonte , Caricato Marco , Valeri Sergio , Coppola Roberto , Tonini Giuseppe 
Published: July 2009

AbstractBACKGROUND:The aim of the current study was the investigation of the value of bevacizumab + 5‐fluorouracil(5–FU)/folinic acid in patients with advanced colorectal cancers who have exhausted standard chemotherapy options.METHODS:The authors included 48 heavily pretreated patients (colon:rectum, 33:15; men:women, 23:25; median age, 63 years; range, 27‐79 years) whose disease had progressed during or within an oxaliplatin‐based first‐line chemotherapy, an irinotecan‐based second‐line regimen, and a third‐line treatment with cetuximab plus weekly irinotecan. Bevacizumab was given at a dose of 5 mg/kg. 5‐FU/folinic acid was administered according to the de Gramont schedule.RESULTS:The response rate was 6.25%, and 30.4% of patients demonstrated stable disease as the best response. The median time to disease progression was 3.5 months (95% confidence interval [95% CI], 2.3‐6.9 months), and the median survival time was 7.7 months (95% CI, 3.9‐11.9 months). The most common grade 3 to 4 side toxicities (graded according to the National Cancer Institute Common Toxicity Criteria [version 2.0]) were: diarrhea (20.8%), fatigue (14.5%), and stomatitis (12.5%). Grade 3 to 4 hemorrhage occurred in 8 patients (16.6%), including 4 cases of bleeding in the gastrointestinal tract. Other relatively common adverse events such as hypertension, thrombosis, and bowel perforation were reported in 50%, 18.7%, and 4.16%, of patients respectively.CONCLUSIONS:The data from the current study suggest a modest but significant clinical benefit of bevacizumab + de Gramont schedule in heavily pretreated colorectal cancer patients. Cancer 2009. © 2009 American Cancer Society.
CMOS current mirror with enhanced input dynamic range
Authors: Wang Hung‐Yu , Jeang Yuan‐Long , Liu Ying‐Chuan , Huang Yu‐Wei 
Published: July 2009

AbstractA novel CMOS cascode current mirror configuration with enhanced input dynamic range is presented. The proposed mirror circuit combines the advantages of wide input swing, wide output swing and large output resistance capability, which make it attractive for practical application. Based on 0.18 µm MOS model parameters, HSPICE simulation results show that the input current ranges from 1 µA to 1 mA with large bandwidth for the proposed circuit. The simulation results confirm the theoretical prediction. Copyright © 2009 John Wiley & Sons, Ltd.
Dysplastic cells in cytological cervical samples show a high incidence of chromosomal abnormalities
Authors: Song Minghao , Ruth Adam , Policht Frank A. , Bubendorf Lukas , Feichter Georg , Kipp Benjamin R. , Halling Kevin C. , Sokolova Irina A. 
Published: July 2009

AbstractChromosomal abnormalities are frequent in most cervical cancers. Amplifications of both the 3q26 (TERC) and 8q24 (MYC) loci have been shown to be prevalent in both high‐grade lesions and invasive cervical carcinoma. Most of these studies have looked at either the histological sample or at the entire cytological population of cells. We have developed a Papanicolaou (Pap) destaining method that allows for the accurate analysis of individual cells that were previously identified by cytopathology as dysplastic. The application of fluorescence in situ hybridization (FISH) was then implemented to determine the chromosomal status of the dysplastic cells in the samples and correlate the two events. Chromosomal abnormality is over a thousand times more frequent in dysplastic cells compared with their morphologically normal counterparts. Diagn. Cytopathol. 2010. © 2009 Wiley‐Liss, Inc.
Evidence‐based guidelines to optimize the selection of antibody panels in cytopathology: Pleural effusions with malignant epithelioid cells
Authors: Westfall Danielle E. , Fan Xuemo , Marchevsky Alberto M. 
Published: July 2009

AbstractThere is no established methodology to help select cost effective antibody panels. We used Bayesian statistics and an evidence‐based pathology (EBP) approach to retrospectively review the use of immunohistochemistry (IHC) in 153 consecutive pleural effusions evaluated in our laboratory from 2005–2007 for the differential diagnosis of malignant mesothelial cells versus carcinoma cells and to estimate the likely site of origin of a carcinoma. The results in this “training” set were used to design antibody panels and test their clinical applicability on a “test set” of 44 pleural effusions collected in early 2008. Cytopathologists had used 6 ± 4.5 IHC tests per case for the diagnosis of malignant mesothelioma (n = 9) and carcinomas of lung (n = 60), breast (n = 47), Müllerian (n = 25), and other origins in the “training set”. The sensitivity and specificity of pleural cytology using all these IHC tests were 32% and 95%, respectively. Sensitivity, specificity and post‐test odds (PTO) of a positive IHC result were calculated for each antibody and by the following classes: malignant mesothelial cells and carcinoma cells by primary site of origin. The antibodies that provided the best PTO to diagnose the most prevalent tumors in our population were included in diagnostic panels for male (calretinin, TTF‐1, PSA and CDX‐2) and female (calretinin, TTF‐1, ER and CA125) patients. These panels provided 100% specificity and 77% and 50% sensitivity, respectively, for the pleural effusions from female and male patients in the “test set.” The use of an EBP approach for test selection in cytopathology is discussed. Diagn. Cytopathol. 2010. © 2009 Wiley‐Liss, Inc.
Update on human polyomavirus BK nephropathy
Authors: Cimbaluk David , Pitelka Lisa , Kluskens Larry , Gattuso Paolo 
Published: July 2009

AbstractPolyomavirus BK (BKV) has ebeen identified as the main cause of polyomavirus‐associated nephropathy, a major cause of renal allograft failure. Although BKV‐associated nephropathy develops in only 2% to 5% of renal transplant recipients, its prognosis when present is very poor, with irreversible graft failure developing in 45% of affected patients. While the use of urine cytology for the detection of decoy cells has been in use for decades, other diagnostic modalities to detect BKV have emerged, including tissue biopsy, polymerase chain reaction, viral culture, and serology. Currently, there is no consensus regarding the laboratory technique best suited for clinical monitoring. This review article will discuss essential and clinical features of polyomavirus, followed by a discussion pertaining to the various diagnostic modalities that contribute to detecting polyomavirus‐associated nephropathy. Diagn. Cytopathol. 2009. © 2009 Wiley‐Liss, Inc.
Fine needle aspiration biopsy of renal mucinous tubular and spindle cell carcinoma: Report of two cases
Authors: Marks‐Jones Deborah A. , Zynger Debra L. , Parwani Anil V. , Cai Guoping 
Published: July 2009

AbstractMucinous tubular and spindle cell carcinoma (MTSCC) is a rare renal tumor. Here we report two cases of MTSCC which were initially evaluated by fine needle aspiration biopsy (FNAB) and followed by surgical resection of the tumors. The cytomorphologic features of MTSCC were characterized by aggregates of relatively uniform, predominantly oval to spindle cells intermixed with abundant metachromatic myxoid matrix. Only rare epithelioid tumor cells with vacuolated cytoplasm were present. Immunohistochemically, the tumor cells were positive for CK7, CK19, CD10, vimentin, E‐cadherin, alpha‐methyl CoA racemase, and negative for CK903 and CK20. EMA and carbonic anhydrase IX immunoreactivity was seen in one of the two cases. Multiple chromosomal losses involving chromosomes 1, 2, 17 and likely chromosome 7 were revealed by fluorescence in situ hybridization (FISH). These cytomorphologic, immunophenotypic, and cytogenetic features were helpful for including this entity in the differential diagnosis of renal cell carcinomas. Diagn. Cytopathol. 2010. © 2009 Wiley‐Liss, Inc.
Effects of therapy in type 1 and type 2 diabetes mellitus with a peptide derived from islet neogenesis associated protein (INGAP)
Authors: Dungan Kathleen M. , Buse John B. , Ratner Robert E. 
Published: July 2009

AbstractBackgroundIslet neogenesis associated protein (INGAP) has beta cell regenerating effects in experimental models.MethodsSubjects with T1DM (N = 63) and T2DM (N = 126) received 300 or 600 mg/day of INGAP peptide in a 90 day, randomized, double‐blind, placebo‐controlled trial.ResultsIn T1DM, on‐treatment Arginine‐stimulated C‐peptide (AUC0–30) significantly increased from baseline in the 600 mg group (p = 0.0058 versus placebo); no significant changes were seen in the 300 mg group. In T2DM, stimulated C‐peptide was significantly better preserved in the 600 mg group compared to placebo at day 120, 30 days after washout (p = 0.031 versus placebo), but did not reach statistical significance during treatment or in the 300 mg group. In T2DM, A1C decreased significantly more in the 600 mg group compared to placebo at day 90 (−0.94% versus −0.47%, respectively, p = 0.009) and day 120, 30 days after washout (−0.73% versus −0.24%, respectively, p = 0.013). This was accompanied by significant reductions in mean glucose. No difference from placebo was detected in the 300 mg group or in T1DM. Injection site reactions were the most common adverse event, occurring in 8 (36%) of placebo, 19 (90%) of 300 mg, and 15 (75%) of 600 mg groups (T1DM) and 14 (33%) of placebo, 27 (64%) of 300 mg, and 29 (69%) of 600 mg groups (T2DM).ConclusionsINGAP peptide increases C‐peptide secretion in T1DM and improves glycaemic control in T2DM. Longer‐term exposure, more frequent dosing, better tolerated formulations or combination with other therapies may be necessary to achieve optimal clinical response. Copyright © 2009 John Wiley & Sons, Ltd.
Back to the roots of modern analytical toxicology: Jean Servais Stas and the Bocarmé murder case
Authors: Wennig Robert 
Published: July 2009

AbstractIn 1850 the Belgian Count Hypolyte Visart de Bocarmé was accused of having killed his brother‐in‐law Gustave Fougnies by poisoning with nicotine. Bocarmé had isolated nicotine from tobacco leaves (Nicotiana tabacum). J. S. Stas (1813–1891) was committed expert and managed to convince the poisoner. He was the first scientist to deproteinize organ tissues by alcohol and could successfully identify nicotine after diethyl ether extraction from the victim's organs. During court trial this identification was challenged by his mentor M. J. B. Orfila from Paris, who had stated 3 years before, that it would never be possible to isolate and identify organic poisons from organ tissues. Copyright © 2009 John Wiley & Sons, Ltd.
A Structural and Magnetic Study of the Series of Double Perovskites Ca2Fe1+xW1–xO6
Authors: Retuerto María , Alonso José Antonio , Martínez‐Lope María Jesús , García‐Hernández Mar , López Carlos A. , Viola María del Carmen , Pedregosa José C. , Fernández‐Díaz María Teresa 
Published: July 2009

AbstractThe synthesis of the Ca2Fe1+xW1–xO6 perovskite solid solution in the range x = 0.13–0.33 by a soft‐chemistry procedure followed by annealing at different temperatures under different reducing atmospheres, and its characterization by X‐ray and neutron diffraction together with magnetic measurements, is reported. The average Fe oxidation state in this series varies smoothly from Fe2.46+ in Ca2Fe1.13W0.87O6 to Fe3+ in Ca2Fe1.33W0.67O6. A detailed description of the crystal structure of four selected members of this series (x = 0.2, 0.23, 0.30, 0.33) is provided. All these compounds crystallize in the space group P21/n, with a ≈ b ≈ √2a0 and c ≈ 2a0, although they show different levels of long‐range ordering between the two different cations placed at the B positions of the A2B′B″O6 perovskite structure. The driving force for this B′/B″ ordering is the charge difference between both kinds of cations, whereby the earlier members of the series, which contain a large proportion of Fe2+, are fully ordered whereas a strong antisite disorder effect is observed for the later members. The evolution of this antisite disordering has a dramatic influence on the magnetic properties across the series. Thus, for x = 0.20, the ferrimagnetic Curie temperature is above 400 K, although the saturation magnetization is very low, since there is a poor coherence between the antiferromagnetic patches formed by Fe–O–Fe interactions between the B′ and B″ positions of the perovskite, whereas the x = 0.33 compound, which has a lower TC of 310 K, shows a considerable saturation magnetization and a surprisingly strong magnetic neutron scattering for such a structurally disordered sample. The observed scattering can be ascribed to the good coherence obtained via Fe–O–Fe superexchange interactions among the Fe‐rich, antiferromagnetically ordered patches that occur naturally thorughout the crystal. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
gem‐Bisphosphonate‐Ended Group Dendrimers: Design and Gadolinium Complexing Properties
Authors: Franc Grégory , Turrin Cédric‐Olivier , Cavero Emma , Costes Jean‐Pierre , Duhayon Carine , Caminade Anne‐Marie , Majoral Jean‐Pierre 
Published: July 2009

AbstractThe synthesis of the first gem‐bisphosphonate‐ended group dendrimers is described using nucleophilic substitution of terminal P(S)Cl2 units of phosphorus dendrimers of generation 1 to 3 with protected aminophenols followed by deprotection of amino groups and Michael addition with vinylidene tetraisopropyl bisphosphonate. These dendrimers were found to act as chelating agents towards Gd ions.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Efficient Synthesis of Modular Amino Acid Derivatives Containing Selenium with Pronounced GPx‐Like Activity
Authors: Alberto Eduardo E. , Soares Letiére C. , Sudati Jéssie H. , Borges Antonio C. A. , Rocha João B. T. , Braga Antonio L. 
Published: July 2009

AbstractNew chiral selenide‐ and diselenide amino acid derivatives have been synthesized. By a simple and efficient two‐step route, these new compounds were obtained from inexpensive and commercially available L‐amino acids. The products, with a highly modular character, were obtained in good to excellent yields. Selected examples were also efficiently used as GPx mimics, catalyzing the reduction of H2O2 to water at the expense of PhSH. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Synthesis and Properties of Oligonucleotides Containing Novel Fluorescent Biaryl Units
Authors: Ueno Yoshihito , Komatsuzaki Shinji , Takasu Keiji , Kawai Satoru , Kitamura Yoshiaki , Kitade Yukio 
Published: July 2009

AbstractWe describe the synthesis and properties of oligonucleotides (ONs) containing biaryl units, which are composed of a bis(hydroxymethyl)benzene residue and a naphthalene or pyrene moiety. We found that by introducing the biaryl units into the ONs, the aromatic chromophores were suitably arrayed in the DNAs. Further, we succeeded in the detection of a single‐base mismatch in RNA by using the ON containing the biaryl unit as a molecular beacon. (© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Selective elimination of amplified CDK4 sequences correlates with spontaneous adipocytic differentiation in liposarcoma
Authors: Hélias‐Rodzewicz Zofia , Pédeutour Florence , Coindre Jean‐Michel , Terrier Philippe , Aurias Alain 
Published: July 2009

AbstractWell‐differentiated and undifferentiated liposarcomas are characterized by high‐level amplifications of chromosome 12 regions including the CDK4 and MDM2 genes. These amplicons are either localized, in well‐differentiated liposarcoma (WDLPS), on extrachromosomal structures (ring or rod chromosomes), or integrated into chromosome arms in undifferentiated tumors. Our results reveal that extrachromosomal amplicons are unstable, and frequently lost by micronucleation. This loss correlates with hypermethylation of eliminated sequences and changes of their replication time. Treatment of cells with demethylating agents during early S‐phase significantly decreases the rate of micronuclei positive for CDK4. We also demonstrate that, in our model, micronuclei are generated during anaphase as a consequence of anaphase abnormalities (chromosome lagging and anaphase bridges). Finally, a dramatic increase of adipocytic differentiation was noted in cells that have eliminated copies of CDK4 gene in micronuclei. These findings provide evidence that, in WDLPS, adipocytic differentiation could be the consequence of CDK4 loss, an event occurring rarely in undifferentiated tumors in which the amplified sequences are integrated into chromosome arms. © 2009 Wiley‐Liss, Inc.
Were genome‐wide linkage studies a waste of time? Exploiting candidate regions within genome‐wide association studies
Authors: Yoo Yun J. , Bull Shelley B. , Paterson Andrew D. , Waggott Daryl , Sun Lei 
Published: July 2009

AbstractA central issue in genome‐wide association (GWA) studies is assessing statistical significance while adjusting for multiple hypothesis testing. An equally important question is the statistical efficiency of the GWA design as compared to the traditional sequential approach in which genome‐wide linkage analysis is followed by region‐wise association mapping. Nevertheless, GWA is becoming more popular due in part to cost efficiency: commercially available 1M chips are nearly as inexpensive as a custom‐designed 10 K chip. It is becoming apparent, however, that most of the on‐going GWA studies with 2,000–5,000 samples are in fact underpowered. As a means to improve power, we emphasize the importance of utilizing prior information such as results of previous linkage studies via a stratified false discovery rate (FDR) control. The essence of the stratified FDR control is to prioritize the genome and maintain power to interrogate candidate regions within the GWA study. These candidate regions can be defined as, but are by no means limited to, linkage‐peak regions. Furthermore, we theoretically unify the stratified FDR approach and the weighted P‐value method, and we show that stratified FDR can be formulated as a robust version of weighted FDR. Finally, we demonstrate the utility of the methods in two GWA datasets: Type 2 diabetes (FUSION) and an on‐going study of long‐term diabetic complications (DCCT/EDIC). The methods are implemented as a user‐friendly software package, SFDR. The same stratification framework can be readily applied to other type of studies, for example, using GWA results to improve the power of sequencing data analyses. Genet. Epidemiol. 34: 107–118, 2010. © 2009 Wiley‐Liss, Inc.
Reliability and validity of the Vanderbilt Head and Neck Symptom Survey: A tool to assess symptom burden in patients treated with chemoradiation
Authors: Murphy Barbara A. , Dietrich Mary S. , Wells Nancy , Dwyer Kathleen , Ridner Sheila H. , Silver Heidi J. , Gilbert Jill , Chung Christine H. , Cmelak Anthony , Burkey Brian , Yarbrough Wendell G. , Sinard Robert , Netterville James 
Published: July 2009

AbstractBackground.We describe the development and validation of the Vanderbilt Head and Neck Symptom Survey (VHNSS), which was designed to screen for tumor‐ and treatment‐specific symptoms in patients with head and neck cancer undergoing concurrent chemoradiation (CCR).Methods.Using a 2‐step card sort method, we identified high‐impact and high‐frequency toxicities in patients with head and neck cancer treated with CCR. This resulted in a 28‐item questionnaire which scores symptoms on a 0 to 10 scale (none to severe). The tool was validated using data collected from 5 supportive care studies comprising a total sample of 332 patients with head and neck cancer.Results.Responses to the VHNSS items demonstrated a very consistent pattern (Cronbach's alpha = 0.943) with each item contributing substantially to the global index. Five symptom subscales were identified including “Nutrition,” “Pain,” “Voice,” “Swallow,” and “Mucous/Dry Mouth.” Each of the cluster scores demonstrated good internal consistency. The pattern of associations between the VHNSS and established tools indicated appropriate convergence and divergence. Comparison of global and subscale scores and objective measure were also in the expected direction providing further evidence of validity.Conclusions.The findings provide support that the VHNSS is a valid and reliable tool to assess head and neck–specific symptom burden and function loss. Further research to evaluate this screening tool as a part of a systems approach to supportive care is warranted. © 2009 Wiley Periodicals, Inc. Head Neck, 2010
Metastases to level IIb in squamous cell carcinoma of the oral cavity: A systematic review and meta‐analysis
Authors: Lea Jane , Bachar Gideon , Sawka Anna M. , Lakra Deepak C. , Gilbert Ralph W. , Irish Jonathan C. , Brown Dale H. , Gullane Patrick J. , Goldstein David P. 
Published: July 2009

AbstractBackground.In this study, a meta‐analysis of level IIb metastases in squamous cell carcinoma (SCC) of the oral cavity was conducted.Methods.Two independent reviewers screened abstracts and full text papers deemed potentially relevant. Data were pooled using a random intercept model.Results.In this analysis, 729 abstracts and 177 full text papers were screened (Kappa statistic 0.8 and 1.0, respectively). A total of 332 patients in 9 papers were included in the analysis. Twenty patients had level IIb metastases (mean, 6%; range, 0% to 10.4%). The pooled percentage of level IIb metastases was 6.0% (95% CI: 3.5–8.6). Only 3 patients with level IIb metastases had isolated nodal disease. Eighty‐five percent of those with level IIb metastases had additional nodal disease (95% CI: 64.0–94.8), with IIa being a common denominator among all.Conclusion.Level IIb nodal metastases are relatively uncommon in previously untreated SCC of the oral cavity (6%). Furthermore, isolated level IIb nodal disease is uncommon. However, given the quality of evidence to date, it is recommended that dissection of level IIb remain the standard of care in oral cavity squamous cell cancer. © 2009 Wiley Periodicals, Inc. Head Neck, 2010
Nutrition impact symptoms: Key determinants of reduced dietary intake, weight loss, and reduced functional capacity of patients with head and neck cancer before treatment
Authors: Kubrak Catherine , Olson Kärin , Jha Naresh , Jensen Louise , McCargar Linda , Seikaly Hadi , Harris Jeffery , Scrimger Rufus , Parliament Matthew , Baracos Vickie E. 
Published: July 2009

AbstractBackground.Our aim was to evaluate the prevalence and relationship of symptoms with reduced dietary intake, weight, and functional capacity in patients with head and neck cancer.Methods.Three hundred forty‐one patients were prospectively screened with the patient‐generated subjective global assessment before treatment. Logistic analysis was used to relate symptoms to reduced dietary intake, weight, and functional capacity. Cumulative hazard analysis was performed to determine the time and risk of weight loss of each symptom. Survival analysis was performed with Cox proportional hazards model.Results.Anorexia, dysphagia, mouth sores, and others were significant predictors of reduced dietary intake and weight. Symptom presence accelerated the time and probability of weight loss. Body mass index ≤18.5 related to overall survival (p value = .001).Conclusions.Symptoms present before treatment may adversely affect the dietary intake, weight, and functional capacity of patients. Symptom treatment and management is critical to weight loss prevention. © 2009 Wiley Periodicals, Inc. Head Neck, 2010