bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nurses provide valuable proxy assessment of the health‐related quality of life of children with Hodgkin disease
Authors: Klaassen Robert J. , Barr Ronald D. , Hughes Joanna , Rogers Paul , Anderson Ronald , Grundy Paul , Ali S. Kaiser , Yanofsky Rochelle , Abla Oussama , Silva Mariana , Carret Anne‐Sophie , Cappelli Mario 
Published: February 2010

AbstractBACKGROUND:We prospectively compared the proxy reporting of health‐related quality of life (HRQL) by parents and nurses of children with Hodgkin disease to see how well they correlated with the children's report.METHODS:Children with all stages of Hodgkin disease, their parents, and the clinic nurse were all asked to complete 4 different HRQL measures at 4 time points: 2 weeks after the first course of chemotherapy, on the third day of the second course of chemotherapy, during the third week of radiation, and 1 year after diagnosis.RESULTS:Fifty‐one patients from 12 centers across Canada were enrolled in the study between May 1, 2002 and March 31, 2005. Two patients were excluded. The children's Pediatric Quality of Life Inventory (PedsQL) generic scores increased from 64 at base line to 81 at the end of the study. There was substantial agreement (intraclass correlation coefficient >0.6) among the participants' scores at most time points except when the child was actively receiving inpatient chemotherapy. At that time, there was only fair to moderate agreement between the child and parent, with the parents on average rating the PedsQL generic score 5 points lower than the child. The nurses still had substantial agreement at that time point with the PedsQL generic and cancer module as well as the EuroQol EQ‐5D visual analogue scale visual analogue scale.CONCLUSIONS:Over the course of treatment for Hodgkin disease, there was substantial agreement among the parent's, nurse's, and children's reported HRQL scores. Nurses contribute valuable additional information as proxy respondents. Cancer 2010. © 2010 American Cancer Society.
Conditions for using FRAP as a quantitative technique—Influence of the bleaching protocol
Authors: Trembecka Dominika O. , Kuzak Mateusz , Dobrucki Jurek W. 
Published: February 2010

AbstractFluorescence recovery after photobleaching (FRAP) is a tool widely used in studies of dynamic behavior of fluorescently‐tagged proteins in live cells. We have analyzed published data on dynamics of various nuclear proteins and note that FRAP protocols and methods of data analysis vary between laboratories. A question arises if the experimental protocol can influence the recovery times. To establish if the FRAP protocol can influence fluorescence half‐recovery times, we used various FRAP protocols and studied the dynamics of a GFP‐tagged H1 (linker) histone. We demonstrate that fluorescence half‐recovery times depend on the bleaching protocol, including the photon flux of the bleaching light. Thus, we conclude that due to differences between protocols and ways of analyzing data, the existing body of information on mobility of various nuclear proteins does not permit direct comparisons between experiments from different laboratories. To exploit a full potential of FRAP as a quantitative technique, there is a need to establish ground rules for photobleaching protocols and adopt a consistent way of data analysis. © 2010 International Society for Advancement of Cytometry
On feasibility examination of DS‐CDMA systems with imperfect successive interference cancellation
Authors: Zhou Zhaorong , Feng Gang , Zhang Yide , Li Lemin 
Published: February 2010

AbstractIn DS‐CDMA systems with the successive interference cancellation (SIC) technique, there areK! possible decoding orders forKactive users and the decoding order has considerable impact on system performance. Once the constraints on the received powers of mobile stations and the bit‐energy‐to‐interference‐power‐spectral‐density ratio requirements are satisfied under some decoding order of SIC, the system is feasible. Otherwise, if the constraints are violated under all possible decoding orders, the system is infeasible. It is highly time‐consuming to examine the system feasibility directly by using the usual exhaustive search method (ESM) for a system with even moderate number of users. In this paper, we propose an efficient approach for examining the feasibility of DS‐CDMA systems with imperfect SIC. The proposed approach has significantly lower computational complexity than that of ESM and thus benefits the quick decisions of admission control and/or scheduling, which are essential for Quality of Service provisioning in DS‐CDMA systems. Furthermore, we theoretically prove that the system under the resultant decoding order obtained by the proposed approach is able to achieve the lowest outage probability among all possible decoding orders. We conduct extensive simulation experiments, and the numerical results validate our analysis and demonstrate the effectiveness of our approach. Copyright © 2010 John Wiley & Sons, Ltd.
Visual experience and age affect synaptic organization in the mushroom bodies of the desert ant Cataglyphis fortis
Authors: Stieb Sara Mae , Muenz Thomas Sebastian , Wehner Rüdiger , Rössler Wolfgang 
Published: February 2010

AbstractDesert ants of the genus Cataglyphis undergo an age‐related polyethism from interior workers involved in brood care and food processing to short‐lived outdoor foragers with remarkable visual navigation capabilities. The quick transition from dark to light suggests that visual centers in the ant's brain express a high degree of plasticity. To investigate structural synaptic plasticity in the mushroom bodies (MBs)—sensory integration centers supposed to be involved in learning and memory—we immunolabeled and quantified pre‐ and postsynaptic profiles of synaptic complexes (microglomeruli, MG) in the visual (collar) and olfactory (lip) input regions of the MB calyx. The results show that a volume increase of the MB calyx during behavioral transition is associated with a decrease in MG numbers in the collar and, less pronounced, in the lip. Analysis of tubulin‐positive profiles indicates that presynaptic pruning of projection neurons and dendritic expansion in intrinsic Kenyon cells are involved. Light‐exposure of dark‐reared ants of different age classes revealed similar effects. The results indicate that this structural synaptic plasticity in the MB calyx is primarily driven by visual experience rather than by an internal program. This is supported by the fact that dark‐reared ants age‐matched to foragers had MG numbers comparable to those of interior workers. Ants aged artificially for up to 1 year expressed a similar plasticity. These results suggest that the high degree of neuronal plasticity in visual input regions of the MB calyx may be an important factor related to behavior transitions associated with division of labor. © 2010 Wiley Periodicals, Inc. Develop Neurobiol 70: 408–423, 2010
An application of Ag(III) complex chemiluminescence system for the determination of enoxacin in capsule and biological fluid
Authors: Chen Peiyun , Sun Hanwen 
Published: February 2010

AbstractAg(III) complex chemiluminescence (CL) system was applied for the determination of enoxacin (ENX). The CL conditions of [Ag(HIO6)2]5−H2SO4ENX systems without any luminescence reagent were investigated and optimized. Under the optimized conditions, the CL intensity was proportional to the concentration of ENX in the range from 6.6 × 10−5 to 3.3 × 10−3 g/L. The limit of detection (s/n = 3) was 2.0 × 10−5 g/L. The recovery of ENX from the spiked pharmaceutical preparations was in the range of 82.9–108% with a relative standard deviation of 1.9–3.0%. For spiked serum and urine samples the recovery of ENX was in the range of 83.7–110% with a relative standard deviation of 1.1–2.8%. The proposed method was applied successfully to the determination of the drug in capsule, serum and urine samples. Copyright © 2010 John Wiley & Sons, Ltd.
Estimating measurement uncertainty in quantitative methods not based on chromatography for doping control purposes
Authors: Van Eenoo P. , Van Renterghem P. , Dimopoulou C. H. , Delbeke F.T. , Georgakopoulos C. G. 
Published: February 2010

AbstractEstimation of measurement uncertainty (MU) for quantitative results is a requirement of ISO/IEC17025. This concept is well established for chromatographic methods in doping control and forensic analysis. For non‐chromatographic methods, however, very few practical methodologies have been published. In this paper, the applicability of a top‐down model, established for estimating uncertainty in chromatography, was evaluated for two other methodologies with different sets of raw data as a starting point. The first case study involves the estimation of MU for the determination of haematological parameters. In this case, a large data set of quality control material and proficiency testing results was available to establish MU. The second case study involves the estimation of MU for the recently approved method for the determination of human growth hormone misuse. In this case the amount of data available to establish MU was limited to results from method validation and a basic set of analysis data. In both cases a methodology based upon long‐term bias, long‐term imprecision and—eventually—a correction for standard impurity is proposed. The proposed methodology can be regarded as a dynamic procedure, which allows re‐evaluation of MU on a regular basis. Finally, a concept for the verification and evaluation of MU estimations using proficiency testing results is proposed. Copyright © 2010 John Wiley & Sons, Ltd.
Synthesis, Cytotoxicity and Antibacterial Studies of p‐Methoxybenzyl‐Substituted and Benzyl‐Substituted N‐Heterocyclic Carbene–Silver Complexes
Authors: Patil Siddappa , Claffey James , Deally Anthony , Hogan Megan , Gleeson Brendan , Menéndez Méndez Luis Miguel , Müller‐Bunz Helge , Paradisi Francesca , Tacke Matthias 
Published: February 2010

Abstractp‐Methoxybenzyl‐substituted and benzyl‐substituted N‐heterocyclic carbene (NHC) [(3a–c) and (6a–c)] precursors were synthesised from the reaction of 1H‐imidazole (1a), 4,5‐dichloro‐1H‐imidazole (1b), and 1H‐benzimidazole (1c) with p‐methoxybenzyl bromide (2) and benzyl bromide (5). These NHC precursors were then treated with silver(I) acetate to yield the NHC–silver complexes [1,3‐bis(4‐methoxybenzyl)imidazol‐2‐ylidene]silver(I) acetate (4a), [4,5‐dichloro‐1,3‐bis(4‐methoxybenzyl)imidazol‐2‐ylidene]silver(I) acetate (4b), [1,3‐bis(4‐methoxybenzyl)benzimidazol‐2‐ylidene]silver(I) acetate (4c), (1,3‐dibenzylimidazol‐2‐ylidene)silver(I) acetate (7a), (1,3‐dibenzyl‐4,5‐dichloroimidazol‐2‐ylidene)silver(I) acetate (7b), and (1,3‐dibenzylbenzimidazol‐2‐ylidene)silver(I) acetate (7c), respectively. The NHC precursor 3c, four NHC–silver complexes 4c and 7a–c were characterised by single‐crystal X‐ray diffraction method. The preliminary antibacterial activity of all the compounds was studied against Gram‐negative bacteria Escherichia coli, and Gram‐positive bacteria Staphylococcus aureus using the Kirby–Bauer disk‐diffusion method. Almost all the NHC–silver complexes have shown high antibacterial activity compared to the NHC precursors. In addition, the NHC–silver complexes had their cytotoxicity investigated through MTT‐based preliminary in vitro testing on the Caki‐1 cell lines in order to determine their IC50 values. NHC–silver complexes 4a–c and 7a–c were found to have IC50 values of 7.3 (+/–6), 12.7(+/–3), 25.2 (+/–5), 2.5 (+/–3), 10.8 (+/–4) and 12.5 (+/–4) μM respectively on the Caki‐1 cell line.
Solid Solution Sr2Sc1+xRe1–xO6 with a Perovskite­‐Like Structure: Phase Transitions and Magnetic Properties
Authors: Mikhailova Daria , Narayanan Narendirakumar , Voss Andrea , Ehrenberg Helmut , Trots Dmytro M. , Ritter Clemens , Eckert Jürgen , Fuess Hartmut 
Published: February 2010

AbstractThe solid solution Sr2Sc1+xRe1–xO6 with a monoclinicperovskite‐like structure has been prepared with the composition range 0 ≤ x ≤ 0.333. When x ≤ 0, other phases compete with the formation of Sr2Sc1+xRe1–xO6. Crystallographic details of Sr2ScReO6 (x = 0) were investigated by neutron and synchrotron powder diffraction in the temperature range 10–1153 K. Upon cooling, Sr2ScReO6 undergoes structural phase transitions: from cubic Fm‐3m to tetragonal I4/m at ca. 800 K and from tetragonal I4/m to monoclinic P21/n at ca. 350 K, and a two‐phase field (monoclinic P21/n + tetragonal I4/m) was observed below 75 K. The latter transition coincides with a magnetic rearrangement from the paramagnetic to antiferromagnetic state. Compositions with x > 0 demonstrate no antiferromagnetic ordering.
Synthesis of α‐Amino Nitriles from Carbonyl Compounds, Amines, and Trimethylsilyl Cyanide: Comparison between Catalyst‐Free Conditions and the Presence of Tin Ion‐Exchanged Montmorillonite
Authors: Wang Jiacheng , Masui Yoichi , Onaka Makoto 
Published: February 2010

AbstractIn the absence of catalysts, the three‐component, one‐pot synthesis of α‐amino nitriles proceeded using various aldehydes and ketones together with amines and trimethylsilyl cyanide (TMSCN) in high yields under neat conditions at room temperature. The addition order of the reagents had a significant influence on the yields of the desired α‐amino nitriles. In contrast, when tin ion‐exchanged montmorillonite (Sn‐Mont), prepared by the ion‐exchange of sodium montmorillonite (Na‐Mont) with a tin tetrachloride solution, was used as a catalyst, the reaction rates significantly increased compared with those without catalysts, and the range of the applicable carbonyl compounds was also extended: structurally diverse aromatic, aliphatic and heteroatom‐containing carbonyl compounds, including sterically hindered ketones as well as aliphatic and aromatic amines, were converted into the desired α‐amino nitriles in good to excellent yields with short reaction times under mild conditions. Sn‐Mont showed a better catalytic activity than proton or other metal ion‐exchanged montmorillonites, supported SnO2 catalysts and the previously reported homogeneous or heterogeneous catalysts. The recovered catalyst was reused several times without loss of catalytic performance. Along with the expansion of the interlayer space of Sn‐Mont, the strong Brønsted acid and Lewis acid nature of Sn‐Mont derived from protons and SnO2 nanoparticles present in the interlayers of Sn‐Mont likely played important and cooperative roles in the high catalytic activity.
The Electropolymerization of 2,6‐Diaminopyridine and Its Application as Mercury Ion Selective Electrode
Authors: Bakhtiarzadeh Fariba , Ab Ghani Sulaiman 
Published: February 2010

AbstractA composite graphite (CG) electrode modified with poly(2,6‐diaminopyridine) (PDAP) was used as solid state‐ion selective electrode for determination of mercury. The electrooxidation of monomer 2, 6 diaminopyridine (DAP) onto CG was accomplished from the 30 mM DAP in 5% H2SO4 and 0.5 M ZnSO4. The electrode displayed Nernstian response with slope of 28.4±1 mV decade−1 in concentration range of 1×10−6 to 1×10−1 M and in solution of pH 3–5. The limit of detection for electrode was 3×10−8 M with response time of 25 s. The electrode was also suitable as an indicator electrode in the potentiometric titration of Hg2+ with iodide.
Direct Electrochemistry and Electrocatalysis of Hemoglobin–TiO2 Whisker Film Modified Glassy Carbon Electrode
Authors: Song Min , Feng Xin , Lu Xiaohua , Wang Xuemei 
Published: February 2010

AbstractIn this study, HbTiO2 whisker nanocomposites are prepared by incorporating TiO2 whisker with hemoglobin (Hb). Our studies illustrate that the self‐assembled HbTiO2 whisker films could efficiently facilitate the direct electron transfer of Hb on glassy carbon electrode (GCE). Moreover, our results demonstrate that the catalytic activity to the reduction of H2O2 could be observed on the HbTiO2 whisker modified GCE and the photovoltaic effect of TiO2 whisker can greatly enhance the detection sensitivity of electrocatalytic reduction.
Highly Sensitive Electrochemical Sensor for Nitric Oxide Using the Self‐Assembled Monolayer of 1,8,15,22‐Tetraaminophthalocyanatocobalt(II) on Glassy Carbon Electrode
Authors: Sivanesan Arumugam , John Swamidoss Abraham 
Published: February 2010

AbstractGlassy carbon (GC) electrode modified with a self‐assembled monolayer (SAM) of 1,8,15,22‐tetraaminophthalocyanatocobalt(II) (4α‐CoIITAPc) was used for the selective and highly sensitive determination of nitric oxide (NO). The SAM of 4α‐CoIITAPc was formed on GC electrode by spontaneous adsorption from DMF containing 1 mM 4α‐CoIITAPc. The SAM showed two pairs of well‐defined redox peaks corresponding to CoIII/CoII and CoIIIPc−1/CoIIIPc−2 in 0.2 M phosphate buffer (PB) solution (pH 2.5). The SAM modified electrode showed excellent electrocatalytic activity towards the oxidation of nitric oxide (NO) by enhancing its oxidation current with 310 mV less positive potential shift when compared to bare GC electrode. In amperometric measurements, the current response for NO oxidation was linearly increased in the concentration range of 3×10−9 to 30×10−9 M with a detection limit of 1.4×10−10 M (S/N=3). The proposed method showed a better recovery for NO in human blood serum samples.
Deformation pattern analysis and tectonic implications of a décollement level within the Central Apennines (Italy)
Authors: Tavani Stefano , Cifelli Francesca 
Published: February 2010

AbstractThis work reports structural and anisotropy of magnetic susceptibility (AMS) measurements from an Upper Miocene décollement level exposed in the central Apennine fold‐and‐thrust belt of Italy. The décollement level is located at the boundary between the Mesozoic‐Cenozoic carbonate sequence and the overlying Miocene‐Pliocene syn‐orogenic siliciclastic sediments and consists of a thick shear zone, mainly affecting detritic limestones, dominated by pressure solution cleavage oblique to the bedding.In the study area, the décollement level was active during the early stages of evolution of the fold‐and‐thrust belt, when deeper décollement levels were active in more internal zones, and accounted for the bulk top‐to‐foreland translation of the siliciclastic portion of the multilayer. The increasing deformation led to the progressive foreland migration of deeper décollement levels (and of thrusts emanating from these levels) that were active in the more internal sectors. Consequently, the Mesozoic‐Cenozoic carbonate sequence was folded and faulted and the studied décollement became inactive and was incorporated into the fold‐and‐thrust belt. Pressure solution cleavage within the décollement became a passive marker that underwent rotations associated with later deformational phases. Results indicate that pressure solution cleavage registers two phases of deformation: (1) during thrusting and folding, when beds were folded and (2) at a later stage, when cleavage was rotated, together with thrusts and folds, about vertical axes. This second stage is interpreted as having been induced by the oblique inversion of a Liassic normal fault, during the Miocene compression. Copyright © 2010 John Wiley & Sons, Ltd.
Tumor‐specific mutation and downregulation of ING5 detected in oral squamous cell carcinoma
Authors: Cengiz Beyhan , Gunduz Esra , Gunduz Mehmet , Beder Levent Bekir , Tamamura Ryo , Bagci Cahit , Yamanaka Noboru , Shimizu Kenji , Nagatsuka Hitoshi 
Published: February 2010

AbstractOur previous study showed high frequency of allelic loss at chromosome 2q37 region in oral cancer. This location contains several candidate tumor suppressor genes such as PPP1R7, ILKAP, DTYMK and ING5. We previously showed 3 members of inhibitor of growth (ING) family, ING1, ING3 and ING4 as tumor suppressor gene in head and neck cancer. As ING5 shows high homology with other members of ING genes including highly conserved carboxy‐terminal plant homeodomain and nuclear localization signal, we first picked up ING5 and examined it as a possible tumor suppressor in oral cancer. For this aim, mutation and mRNA expression status of ING5 in paired normal and oral squamous cell carcinoma samples were examined by reverse transcription polymerase chain reaction (RT‐PCR) and sequencing. Three missense mutations located within leucine zipper like (LZL) finger and novel conserved region (NCR) domains in ING5 protein were detected, probably abrogating its normal function. We also found 5 different alternative splicing variants of ING5. Then, we examined mRNA level of ING5 by quantitative real time reverse transcription polymerase chain reaction (qRT‐PCR) analysis, which demonstrated decreased expression of ING5 mRNA in 61% of the primary tumors as compared to the matched normal samples. In conclusion, tumor‐specific mutation and downregulation of ING5 mRNA suggested it as a tumor suppressor gene in oral squamous cell carcinoma.
Characteristic methylation profile in CpG island methylator phenotype‐negative distal colorectal cancers
Authors: An Byonggu , Kondo Yutaka , Okamoto Yasuyuki , Shinjo Keiko , Kanemitsu Yukihide , Komori Koji , Hirai Takashi , Sawaki Akira , Tajika Masahiro , Nakamura Tsuneya , Yamao Kenji , Yatabe Yasushi , Fujii Makiko , Murakami Hideki , Osada Hirotaka , Tani Tohru , Matsuo Keitaro , Shen Lanlan , Issa Jean‐Pierre J. , Sekido Yoshitaka 
Published: February 2010

AbstractAberrant DNA methylation is involved in colon carcinogenesis. Although the CpG island methylator phenotype (CIMP) is defined as a subset of colorectal cancers (CRCs) with remarkably high levels of DNA methylation, it is not known whether epigenetic processes are also involved in CIMP‐negative tumors. We analyzed the DNA methylation profiles of 94 CRCs and their corresponding normal‐appearing colonic mucosa with 11 different markers, including the five classical CIMP markers. The CIMP markers were frequently methylated in proximal CRCs (p < 0.01); however, RASSF1A methylation levels were significantly higher in distal CRCs, the majority of which are CIMP‐negative (p < 0.05). Similarly, methylation levels of RASSF1A and SFRP1 in the normal‐appearing mucosae of distal CRC cases were significantly higher than those in the proximal CRC cases (p < 0.05). They were also positively correlated with age (RASSF1A, p < 0.01; SFRP1, p < 0.01). Microarray‐based genome‐wide DNA methylation analysis of 18 CRCs revealed that 168 genes and 720 genes were preferentially methylated in CIMP‐negative distal CRCs and CIMP‐positive CRCs, respectively. Interestingly, more than half of the hypermethylated genes in CIMP‐negative distal CRCs were also methylated in the normal‐appearing mucosae, indicating that hypermethylation in CIMP‐negative distal CRCs is more closely associated with age‐related methylation. By contrast, more than 60% of the hypermethylated genes in CIMP‐positive proximal CRCs were cancer specific (p < 0.01). These data altogether suggest that CpG island promoters appear to be methylated in different ways depending on location, a finding which may imply the presence of different mechanisms for the acquisition of epigenetic changes during colon tumorigenesis.