bluuebunny/crossref_metadata_2025_split huggingface dataset:
Integrating NWP Forecasts and Observation Data to Improve Nowcasting Accuracy
Authors: Huang Laura X. , Isaac George A. , Sheng Grant 
Published: April 2012

Abstract
This study addresses the issue of improving nowcasting accuracy by integrating several numerical weather prediction (NWP) model forecasts with observation data. To derive the best algorithms for generating integrated forecasts, different integration methods were applied starting with integrating the NWP models using equal weighting. Various refinements are then successively applied including dynamic weighting, variational bias correction, adjusted dynamic weighting, and constraints using current observation data. Three NWP models—the Canadian Global Environmental Multiscale (GEM) regional model, the GEM Limited Area Model (LAM), and the American Rapid Update Cycle (RUC) model—are used to generate the integrated forecasts. Verification is performed at two Canadian airport locations [Toronto International Airport (CYYZ), in Ontario, and Vancouver International Airport (CYVR), in British Columbia] over the winter and summer seasons. The results from the verification for four weather variables (temperature, relative humidity, and wind speed and gust) clearly show that the integrated models with new refinements almost always perform better than each of the NWP models individually and collectively. When the integrated model with innovative dynamic weighting and variational bias correction is further updated with the most current observation data, its performance is the best among all models, for all the selected variables regardless of location and season. The results of this study justify the use of integrated NWP forecasts for nowcasting provided they are properly integrated using appropriate and specifically designed rules and algorithms.
Depictions of Children in the Apocryphal Infancy Gospels
Authors: Burke Tony 
Published: April 2012

The apocryphal infancy gospels (such as the Infancy Gospel of Thomas and the Protoevangelium of James) seem at first look to be ideal sources for the study of children and childhood in Early Christianity. They all feature depictions of Jesus as an infant and/or a child; some tell similar tales of other eminent Christian figures, such as Mary of Nazareth and John the Baptist. Few of these texts, however, can be considered “early” texts (i.e., 2nd–3rd centuries) and even those we can confidently date to this period are of limited value for the study of children. One text remains useful for this endeavor: the Infancy Gospel of Thomas. And in recent years, several scholars have looked seriously at the gospel for what it can tell us about the experiences of children in antiquity. Yet, even this text must be approached with caution for it has more to say about how adults of the time wanted children to be than what they truly were. Les évangiles de l’enfance apocryphes (comme l’Évangile de l’enfance selon Thomas et le Protévangile de Jacques) semblent à première vue comme des sources idéales pour l’étude des enfants et l’enfance au début du christianisme. Ils ont tous des représentations de Jésus comme un bébé et / ou un enfant, certains racontent des histoires similaires des autres éminentes figures chrétiennes, comme Marie de Nazareth et Jean le Baptiste. Peu de ces textes, cependant, peuvent être considérés tôt (par exemple, 2–3e siècles) et même ceux que nous pouvons en toute confiance dater à cette période sont d’une valeur limitée pour l’étude des enfants. Un texte reste utile pour cette tâche : l’Évangile de l’enfance selon Thomas. Et ces dernières années, plusieurs chercheurs se sont penchés sérieusement sur l’évangile pour ce qu’il peut nous dire au sujet des expériences des enfants dans l’antiquité. Pourtant, même ce texte doit être abordé avec prudence car il a plus à dire sur les attentes des adultes de l’époque aux enfants que ce qu’ils étaient vraiment.
Intellectual disability, consumerism and identity
Authors: McClimens Alex , Hyde Martin 
Published: April 2012

Here we consider the consumer society as it currently exists in the UK and examine its relationship and relevance to the population of individuals with intellectual disability. We do this through a reading of the associated literature on theories of shopping and consumption which we then contrast with research evidence as it applies to the lives of people with intellectual disability. By brining together these two perspectives we hope to shine some light on ideas around identity and choice. We then transfer these arguments to the health and social care sector. Here we ask whether an economic model which has been exposed as divisive and exclusionary should be used in the administration of social secutity benefits of the kind accesed by people with a range of disabilities. We conclude that the unchallenged advance of marketisation within health and social care may benefit those who are financially able but for those who are economically disadvantaged the choices offered are illusory at best.
Multilocus genetics to reconstruct aeromonad evolution
Authors: Roger Frédéric , Marchandin Hélène , Jumas-Bilak Estelle , Kodjo Angeli ,   the colBVH study group, Lamy Brigitte 
Published: April 2012

Abstract

Background

Aeromonas spp. are versatile bacteria that exhibit a wide variety of lifestyles. In an attempt to improve the understanding of human aeromonosis, we investigated whether clinical isolates displayed specific characteristics in terms of genetic diversity, population structure and mode of evolution among Aeromonas spp. A collection of 195 Aeromonas isolates from human, animal and environmental sources was therefore genotyped using multilocus sequence analysis (MLSA) based on the dnaK, gltA, gyrB, radA, rpoB, tsf and zipA genes.

Results
The MLSA showed a high level of genetic diversity among the population, and multilocus-based phylogenetic analysis (MLPA) revealed 3 major clades: the A. veronii, A. hydrophila and A. caviae clades, among the eleven clades detected. Lower genetic diversity was observed within the A. caviae clade as well as among clinical isolates compared to environmental isolates. Clonal complexes, each of which included a limited number of strains, mainly corresponded to host-associated subsclusters of strains, i.e., a fish-associated subset within A. salmonicida and 11 human-associated subsets, 9 of which included only disease-associated strains. The population structure was shown to be clonal, with modes of evolution that involved mutations in general and recombination events locally. Recombination was detected in 5 genes in the MLSA scheme and concerned approximately 50% of the STs. Therefore, these recombination events could explain the observed phylogenetic incongruities and low robustness. However, the MLPA globally confirmed the current systematics of the genus Aeromonas.

Conclusions
Evolution in the genus Aeromonas has resulted in exceptionally high genetic diversity. Emerging from this diversity, subsets of strains appeared to be host adapted and/or “disease specialized” while the A. caviae clade displayed an atypical tempo of evolution among aeromonads. Considering that A. salmonicida has been described as a genetically uniform pathogen that has adapted to fish through evolution from a variable ancestral population, we hypothesize that the population structure of aeromonads described herein suggested an ongoing process of adaptation to specialized niches associated with different degrees of advancement according to clades and clusters.
Spatial and temporal dynamics of malaria transmission in rural Western Kenya
Authors: Amek Nyaguara , Bayoh Nabie , Hamel Mary , Lindblade Kim A , Gimnig John E , Odhiambo Frank , Laserson Kayla F , Slutsker Laurence , Smith Thomas , Vounatsou Penelope 
Published: April 2012

AbstractBackgroundUnderstanding the relationship betweenPlasmodium falciparummalaria transmission and health outcomes requires accurate estimates of exposure to infectious mosquitoes. However, measures of exposure such as mosquito density and entomological inoculation rate (EIR) are generally aggregated over large areas and time periods, biasing the outcome-exposure relationship. There are few studies examining the extent and drivers of local variation in malaria exposure in endemic areas.MethodsWe describe the spatio-temporal dynamics of malaria transmission intensity measured by mosquito density and EIR in the KEMRI/CDC health and demographic surveillance system using entomological data collected during 2002–2004. Geostatistical zero inflated binomial and negative binomial models were applied to obtain location specific (house) estimates of sporozoite rates and mosquito densities respectively. Model-based predictions were multiplied to estimate the spatial pattern of annual entomological inoculation rate, a measure of the number of infective bites a person receive per unit of time. The models included environmental and climatic predictors extracted from satellite data, harmonic seasonal trends and parameters describing space-time correlation.ResultsAnopheles gambiaes.l was the main vector species accounting for 86 % (n = 2309) of the total mosquitoes collected with the remainder beingAnopheles funestus. Sixty eight percent (757/1110) of the surveyed houses had no mosquitoes. Distance to water bodies, vegetation and day temperature were strongly associated with mosquito density. Overall annual point estimates of EIR were 6.7, 9.3 and 9.6 infectious bites per annum for 2002, 2003 and 2004 respectively. Monthly mosquito density and EIR varied over the study period peaking in May during the wet season each year. The predicted and observed densities of mosquitoes and EIR showed a strong seasonal and spatial pattern over the study area.ConclusionsSpatio-temporal maps of malaria transmission intensity obtained in this study are not only useful in understanding variability in malaria epidemiology over small areas but also provide a high resolution exposure surface that can be used to analyse the impact of transmission on malaria related and all-cause morbidity and mortality.
Bias in effect size of systemic lupus erythematosus susceptibility loci across Europe: a case-control study
Authors: Alonso-Perez Elisa , Suarez-Gestal Marian , Calaza Manuel , Sebastiani Gian Domenico , Pullmann Rudolf , Papasteriades Chryssa , Kovacs Attila , Skopouli Fotini N , Bijl Marc , Suarez Ana , Marchini Maurizio , Migliaresi Sergio , Carreira Patricia , Ordi-Ros Josep , Witte Torsten , Ruzickova Sarka , Santos Maria Jose , Barizzone Nadia , Blanco Francisco J , Lauwerys Bernard R , Gomez-Reino Juan J , Gonzalez Antonio ,   the European Consortium of SLE DNA Collections
Published: April 2012

Abstract
Introduction
We aimed to investigate whether the effect size of the systemic lupus erythematosus (SLE) risk alleles varies across European subpopulations.

Methods
European SLE patients (n = 1,742) and ethnically matched healthy controls (n = 2,101) were recruited at 17 centres from 10 different countries. Only individuals with self-reported ancestry from the country of origin were included. In addition, participants were genotyped for top ancestry informative markers and for 25 SLE associated SNPs. The results were used to compare effect sizes between the Central Eureopan and Southern European subgroups.

Results
Twenty of the 25 SNPs showed independent association with SLE, These SNPs showed a significant bias to larger effect sizes in the Southern subgroup, with 15/20 showing this trend (P = 0.019) and a larger mean odds ratio of the 20 SNPs (1.46 vs. 1.34, P = 0.02) as well as a larger difference in the number of risk alleles (2.06 vs. 1.63, P = 0.027) between SLE patients and controls than for Central Europeans. This bias was reflected in a very significant difference in the cumulative genetic risk score (4.31 vs. 3.48, P = 1.8 × 10-32). Effect size bias was accompanied by a lower number of SLE risk alleles in the Southern subjects, both patients and controls, the difference being more marked between the controls (P = 1.1 × 10-8) than between the Southern and Central European patients (P = 0.016). Seven of these SNPs showed significant allele frequency clines.

Conclusion
Our findings showed a bias to larger effect sizes of SLE loci in the Southern Europeans relative to the Central Europeans together with clines of SLE risk allele frequencies. These results indicate the need to study risk allele clines and the implications of the polygenic model of inheritance in SLE.
Anti-MJ/NXP-2 autoantibody specificity in a cohort of adult Italian patients with polymyositis/dermatomyositis
Authors: Ceribelli Angela , Fredi Micaela , Taraborelli Mara , Cavazzana Ilaria , Franceschini Franco , Quinzanini Marzia , Tincani Angela , Ross Steven J , Chan Jason YF , Pauley Brad A , Chan Edward KL , Satoh Minoru 
Published: April 2012

Abstract

Introduction

Autoantibodies in patients with polymyositis/dermatomyositis (PM/DM) are associated with unique subsets, clinical course and outcome. Anti-MJ antibodies, which recognize the nuclear protein NXP-2/MORC3, are reported in ~25% of juvenile DM. Prevalence and clinical significance of anti-MJ antibodies in adult Italian PM/DM patients were studied.

Methods

Sera from 58 consecutive adult Italian PM/DM patients were analyzed by immunoprecipitation of 35S-labeled K562 cells extract, ELISA (anti-MJ, Jo-1), Western blot and indirect immunofluorescence. Clinical associations were analyzed using information from medical charts.

Results

Anti-MJ antibodies were the most prevalent specificity (17%) found mainly in DM (30%, 8 cases) vs 8% of PM (2 cases, P = 0.02). Comparing 10 anti-MJ (+) vs 48 anti-MJ (-) cases, DM was more common (P = 0.03), and age at onset was younger in anti-MJ (+) (P = 0.0006). In anti-MJ (+), heliotrope rash (P = 0.01) and calcinosis (P = 0.09) were more frequent. None of them had heart or lung involvement, or malignancy. Myopathy in anti-MJ (+) patients responded well to therapy and none of them had elevated CPK at last visit (0% vs 25% in anti-MJ (-)). Only 60% of anti-MJ (+) showed immunofluorescent nuclear dots staining, despite PML localization of NXP-2/MORC3.

Conclusions

Anti-MJ antibodies are the most frequent specificity in our cohort of adult Italian PM/DM. Anti-MJ (+) were associated with young onset DM, calcinosis, no internal organ involvement and good response of myopathy to therapy. Anti-MJ reported in juvenile DM is also found in adult PM/DM, and could be a new useful biomarker.
The effect of three years of TNF alpha blocking therapy on markers of bone turnover and their predictive value for treatment discontinuation in patients with ankylosing spondylitis: a prospective longitudinal observational cohort study
Authors: Arends Suzanne , Spoorenberg Anneke , Houtman Pieternella M , Leijsma Martha K , Bos Reinhard , Kallenberg Cees GM , Groen Henk , Brouwer Elisabeth , van der Veer Eveline 
Published: April 2012

Abstract
Introduction
The aim of this study was to investigate the effect of three years of tumor necrosis factor-alpha (TNF-α) blocking therapy on bone turnover as well as to analyze the predictive value of early changes in bone turnover markers (BTM) for treatment discontinuation in patients with ankylosing spondylitis (AS).

Methods
This is a prospective cohort study of 111 consecutive AS outpatients who started TNF-α blocking therapy. Clinical assessments and BTM were assessed at baseline, three and six months, as well as at one, two, and three years. Z-scores of BTM were calculated to correct for age and gender. Bone mineral density (BMD) was assessed yearly.

Results
After three years, 72 patients (65%) were still using their first TNF-α blocking agent. In these patients, TNF-α blocking therapy resulted in significantly increased bone-specific alkaline phosphatase, a marker of bone formation; decreased serum collagen-telopeptide (sCTX), a marker of bone resorption; and increased lumbar spine and hip BMD compared to baseline. Baseline to three months decrease in sCTX Z-score (HR: 0.394, 95% CI: 0.263 to 0.591), AS disease activity score (ASDAS; HR: 0.488, 95% CI: 0.317 to 0.752), and physician's global disease activity (HR: 0.739, 95% CI: 0.600 to 0.909) were independent inversely related predictors of time to treatment discontinuation because of inefficacy or intolerance. Early decrease in sCTX Z-score correlated significantly with good long-term response regarding disease activity, physical function and quality of life.

Conclusions
Three years of TNF-α blocking therapy results in a bone turnover balance that favors bone formation, especially mineralization, in combination with continuous improvement of lumbar spine BMD. Early change in sCTX can serve as an objective measure in the evaluation of TNF-α blocking therapy in AS, in addition to the currently used more subjective measures.
Nebulized anticoagulants for acute lung injury - a systematic review of preclinical and clinical investigations
Authors: Tuinman Pieter R , Dixon Barry , Levi Marcel , Juffermans Nicole P , Schultz Marcus J 
Published: April 2012

Abstract
Background
Data from interventional trials of systemic anticoagulation for sepsis inconsistently suggest beneficial effects in case of acute lung injury (ALI). Severe systemic bleeding due to anticoagulation may have offset the possible positive effects. Nebulization of anticoagulants may allow for improved local biological availability and as such may improve efficacy in the lungs and lower the risk of systemic bleeding complications.

Method
We performed a systematic review of preclinical studies and clinical trials investigating the efficacy and safety of nebulized anticoagulants in the setting of lung injury in animals and ALI in humans.

Results
The efficacy of nebulized activated protein C, antithrombin, heparin and danaparoid has been tested in diverse animal models of direct (for example, pneumonia-, intra-pulmonary lipopolysaccharide (LPS)-, and smoke inhalation-induced lung injury) and indirect lung injury (for example, intravenous LPS- and trauma-induced lung injury). Nebulized anticoagulants were found to have the potential to attenuate pulmonary coagulopathy and frequently also inflammation. Notably, nebulized danaparoid and heparin but not activated protein C and antithrombin, were found to have an effect on systemic coagulation. Clinical trials of nebulized anticoagulants are very limited. Nebulized heparin was found to improve survival of patients with smoke inhalation-induced ALI. In a trial of critically ill patients who needed mechanical ventilation for longer than two days, nebulized heparin was associated with a higher number of ventilator-free days. In line with results from preclinical studies, nebulization of heparin was found to have an effect on systemic coagulation, but without causing systemic bleedings.

Conclusion
Local anticoagulant therapy through nebulization of anticoagulants attenuates pulmonary coagulopathy and frequently also inflammation in preclinical studies of lung injury. Recent human trials suggest nebulized heparin for ALI to be beneficial and safe, but data are very limited.
Metabolomic analysis of rat serum in streptozotocin-induced diabetes and after treatment with oral triethylenetetramine (TETA)
Authors: Ugarte Marta , Brown Marie , Hollywood Katherine A , Cooper Garth J , Bishop Paul N , Dunn Warwick B 
Published: April 2012

Abstract
Background
The prevalence, and associated healthcare burden, of diabetes mellitus is increasing worldwide. Mortality and morbidity are associated with diabetic complications in multiple organs and tissues, including the eye, kidney and cardiovascular system, and new therapeutics to treat these complications are required urgently. Triethylenetetramine (TETA) is one such experimental therapeutic that acts to chelate excess copper (II) in diabetic tissues and reduce oxidative stress and cellular damage.

Methods
Here we have performed two independent metabolomic studies of serum to assess the suitability of the streptozotocin (STZ)-induced rat model for studying diabetes and to define metabolite-related changes associated with TETA treatment. Ultraperformance liquid chromatography-mass spectrometry studies of serum from non-diabetic/untreated, non-diabetic/TETA-treated, STZ-induced diabetic/untreated and STZ-induced diabetic/TETA-treated rats were performed followed by univariate and multivariate analysis of data.

Results
Multiple metabolic changes related to STZ-induced diabetes, some of which have been reported previously in other animal and human studies, were observed, including changes in amino acid, fatty acid, glycerophospholipid and bile acid metabolism. Correlation analysis suggested that treatment with TETA led to a reversal of diabetes-associated changes in bile acid, fatty acid, steroid, sphingolipid and glycerophospholipid metabolism and proteolysis.

Conclusions
Metabolomic studies have shown that the STZ-induced rat model of diabetes is an appropriate model system to undertake research into diabetes and potential therapies as several metabolic changes observed in humans and other animal models were also observed in this study. Metabolomics has also identified several biological processes and metabolic pathways implicated in diabetic complications and reversed following treatment with the experimental therapeutic TETA.
Revealing details: whole mount microRNA in situ hybridization protocol for zebrafish embryos and adult tissues
Authors: Lagendijk Anne Karine , Moulton Jon D. , Bakkers Jeroen 
Published: April 2012

Summary
Non-coding microRNA (miRNA) molecules bind their target mRNAs and thereby modulate the amount of protein produced. To understand the significance of a potential miRNA-mRNA interaction, temporal and spatial information on miRNA and mRNA expression is essential. Here, we provide a detailed protocol for miRNA whole mount in situ hybridization. We introduce the use of Morpholino based oligos as antisense probes for miRNA detection, in addition to the current “gold standard” locked nucleic acid (LNA) probes. Furthermore we have modified existing miRNA in situ protocols thereby improving both sensitivity and resolution of miRNA visualization in whole zebrafish embryos and adult tissues.
Serum 25 hydroxy-vitamin D does not exhibit an acute phase reaction after acute myocardial infarction
Authors: Barth J H , Field H P , Mather A N , Plein S 
Published: April 2012

Background There is growing epidemiological evidence linking serum 25 hydroxy-vitamin D (25(OH)D) concentrations to outcome in cardiovascular and other diseases. We have studied patients with acute myocardial infarction (AMI) to determine if they exhibit an acute phase reaction affecting 25(OH)D. Methods Patients ( n= 32) with first AMI who had been treated with primary percutaneous coronary intervention within 12 h of symptom onset had venous blood samples taken two days, one week, one month and three months after presentation. Samples were analysed for troponin I, C-reactive protein (CRP) and 25(OH)D. Results All patients had significant rises in troponin confirming the myocardial damage and CRP, both of which resolved by 28 days. In contrast, 25(OH)D remained unchanged throughout the 90-day observation period with a median concentration of 46 nmol/L. Conclusion Serum 25(OH)D does not change after AMI and is likely to be a reliable marker of vitamin D status in patients with cardiovascular disease.