bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Case of 3q29 Microdeletion Syndrome Involving Oral Cleft Inherited from a Nonaffected Mosaic Parent: Molecular Analysis and Ethical Implications
Authors: Petrin Aline L. , Daack-Hirsch Sandra , L'heureux Jamie , Murray Jeffrey C. 
Published: May 2010

Objective The objective of this study was to use array comparative genomic hybridization to detect causal microdeletions in samples of subjects with cleft lip and palate. Subjects We analyzed DNA samples from a male patient and his parents seen during surgical screening for an Operation Smile medical mission in the Philippines. Method We used Affymetrix® Genome-Wide Human SNP Array 6.0 followed by sequencing and quantitative polymerase chain reaction using SYBR Green I dye. Results We report the second case of 3q29 microdeletion syndrome including cleft lip with or without cleft palate and the first case of this microdeletion syndrome inherited from a phenotypically normal mosaic parent. Conclusions Our findings confirm the usefulness of a comparative genomic hybridization to detect causal microdeletions and indicate that parental somatic mosaicism should be considered in healthy parents for genetic counseling of the families. We discuss important ethical implications of sharing health impact results from research studies with the participant families.
Cephalometric Analysis of Craniofacial Malformations in Newborn Mice with Cleft Palate Induced by Retinoic Acid
Authors: Chen Mu , Huang Hong-Zhang , Zeng Dong-Lin , Wang Da-Wei 
Published: May 2010

Objective To determine changes on craniofacial growth morphometrically in newborn mice with cleft palate induced by retinoic acid. Design, Setting, Participants, Interventions Gestation day 10 or 12 pregnant female C57BL/6N mice were given a single dose of all-trans retinoic acid (atRA) by gastric intubations via oral gavage. Sixty newborn mice with cleft palate (CP), 52 without CP from the experimental group, and 30 without CP from the control group were collected, and lateral cephalograms were taken of all of the mice. Main Outcome Measures Cephalometric analysis of the craniofacial skeleton was performed by means of a personal computer. Results Inhibition of craniofacial growth was found in the experimental groups but not in the control groups. In the maxillary bone and mandible, the amount of growth was significantly reduced. Conclusions These results suggest that craniofacial growth is inhibited in newborn mice with cleft palate induced by retinoic acid.
Novel Use of Osmotic Tissue Expanders to Treat Difficult Anterior Palatal Fistulas
Authors: Jenq Tina F. , Hilliard Stuart M. , Kuang Anna A. 
Published: May 2010

Objective Novel use of osmotic tissue expanders to recruit local palatal mucoperiosteum for the treatment of difficult anterior palatal fistulas. Design This is a retrospective review of our experience with osmotic tissue expanders for closure of secondary palatal fistulas. Setting The study occurred at Oregon Health and Science University, a tertiary care level academic hospital. Patients, Participants All patients were treated for combined cleft lip and palate by the Oregon Health and Science University Craniofacial Disorders multidisciplinary team. They were determined to be appropriate candidates due to the anterior location of the fistula and symptomatic nature. Patients complained of significant nasal regurgitation of liquid and solids. Interventions All patients underwent a two-stage procedure under general anesthesia. The first stage was placement of the expanders. The second stage was 1 week later, with removal of the expanders, palatal revision, and closure of the oronasal fistula. Main Outcome Measure The primary outcome measures determined before data collection were treatment of symptoms and decreased size of fistula. Results Seven patients with palatal fistulas were treated with osmotic tissue expanders. Five patients had complete closure of the fistula. Two patients demonstrated slit-like fistulas that were no longer symptomatic and were amenable to closure at time of alveolar bone grafting. No complications were observed. Conclusions The use of osmotic tissue expanders is a viable alternative for repair of large anterior palatal fistulas, especially in a scarred or previously operated palate. Patients were also no longer symptomatic.
Variations in Velopharyngeal Structures between Upright and Supine Positions Using Upright Magnetic Resonance Imaging
Authors: Perry Jamie L. 
Published: May 2010

Objective No studies have used MRI to compare the dimensional changes of the velopharyngeal musculature between upright and supine positions. The purpose of this study is to provide a comparison between structures of the velopharyngeal mechanism while in the supine and upright positions during rest and sustained speech productions of four female subjects. Methods Four healthy white female subjects between 30 and 36 years of age (mean, 32.5 ± 1.75 years) were imaged using a 0.6-tesla open-type multi-position MRI scanner. Subjects produced two speech tasks (/i/ and /s/) in the two body positions. Results Velar measures (length, thickness, and height), pharyngeal measures (retrovelar and retrolingual), and levator muscle measures (length and angles of origin) demonstrated a small variation between upright and supine positions. Differences in velar height during production of /i/ between the two positions was significant for all subjects. In most cases, the velar thickness remained nearly the same (group mean difference was between –0.2 and 1.2 mm). Group means demonstrated an average levator muscle shortening from upright to supine of 2.8 mm at rest, 2.0 mm during /i/ production, and 2.3 mm during /s/ production. Percentage of levator shortening were 17% for /i/ production and 21% for /s/ production, which were independent of body position. Conclusions Overall, gravity had a minimal effect on velar thickness, velar length, velar height, levator muscle length, angles of origin, and pharyngeal dimensions. Differences between the two body positions (upright and supine) were not significant during rest or during production of /i/ and /s/, with the exception of velar height during /i/ production.
Fenofibrate in Primary Biliary Cirrhosis: A Pilot Study
Authors: Liberopoulos E.N , Florentin M , Elisaf M.S , Mikhailidis D.P , Tsianos E 
Published: May 2010

Background:Most patients with primary biliary cirrhosis (PBC) are treated with ursodeoxycholic acid (UDCA); however, some do not respond fully. PBC is also associated with dyslipidemia, but a link with vascular risk has not been confirmed.Methods and Results:In this study we compared UDCA monotherapy with fenofibrate plus UDCA in PBC patients with incomplete biochemical response to UDCA monotherapy for ≥ 8 months. Ten patients (57.2±13.3 years old) with PBC and persistent elevations of liver enzymes after treatment with UDCA (600 mg/day) were randomized to continue UDCA (4 patients) or to receive micronized fenofibrate (200 mg/day) plus UDCA (6 patients) for 8 weeks. Significant reductions in total cholesterol, triglycerides and non-high density lipoprotein cholesterol were observed in the combination treatment group. The serum activities of alkaline phosphatase, gamma-glutamyl transpeptidase and alanine aminotranferase also decreased in this group compared with baseline (-32.6%; p=0.012, -44%; p=0.031 and -16.9%; p=0.029, respectively). In contrast, no significant alterations in liver enzymes or lipid profile were observed in patients who continued UDCA monotherapy. The changes in the lipid and enzyme variables differed significantly (p<0.03) between the 2 groups. Fenofibrate was well tolerated.Conclusions:The administration of fenofibrate plus UDCA seems to be safe and may improve lipid and liver indices in patients with PBC who do not respond fully to UDCA monotherapy. Whether the improved lipid profile translates into a decreased risk of vascular events remains to be established.
Low Bone Mass and Osteoporosis in Women Referring themselves to Dual
X-Ray Absorptiometry - Experience with a Health Promotion Action
Authors: Goerres Gerhard W. , Swanenburg Jaap , Uebelhart Daniel 
Published: May 2010

Aims:
This retrospective analysis was done to describe the difference in the prevalence of osteoporosis/low
bone mass between women referring themselves to bone mineral density (BMD) testing with dual X-ray absorptiometry
(DXA) and women referred by their family practitioner.

Methods:
Women were recruited by a health promotion action in a Swiss weekly periodical and compared with female
patients sent by their physician for DXA testing for various medical indications during the same period. Patients under
steroid treatment, known previous fracture and undergoing follow-up for low bone mass were excluded. Self referred
women were compared to female patients aged 40 years and older and the same evaluation was repeated for women aged
65 and older.

Results:
No differences were found in the prevalence of osteoporosis /low bone mass in women referred by their physician
vs those who were self referred. However, a significant difference was found with age: the self referred women were 63.1
± 8.6 years of age whereas the patient group’s mean age was 59.7 ± 9.4 (p=0.0001, 95% CI of the difference: 21 – 61
years).

Conclusion:
We suggest that health promotion actions might be able to recruit the correct candidates for BMD testing,
since we found no significant difference in the prevalence of osteoporosis/ low bone mass between self referred and
physician referred women. Our data further suggest that physicians may react earlier on their patient’s risk profiles than
the time frame of action by the self-referred women.
Determination of ribosomal internal transcribed spacer 2 (ITS2) interspecific markers in Fasciola hepatica, Fascioloides magna, Dicrocoelium dendriticum and Paramphistomum cervi (Trematoda), parasites of wild and domestic ruminants
Authors: Bazsalovicsová E. , Králová-Hromadová I. , Špakulová M. , Reblánová M. , Oberhauserová K. 
Published: May 2010

Abstract
The species-specific ribosomal internal transcribed spacer 2 (ITS2) markers were designed for PCR-based molecular differentiation of Fasciola hepatica, Fascioloides magna, Dicrocoelium dendriticum and Paramphistomum cervi, liver and stomach flukes of domestic and free living ruminants. Complete ITS2 sequences were obtained for D. dendriticum and P. cervi, for the later species, ITS2 structure was determined for the first time. Intraspecific variation within geographically distant populations was found to be either very low (F. hepatica; D. dendriticum) or even absent (F. magna; P. cervi). ITS2 regions with the absence of intraspecific polymorphisms but with interspecific sequence heterogeneity were applied for design of speciesspecific primers. The specificity of developed primers was tested on genomic DNA isolated from adult individuals of studied fluke species. Application of the primers is of particular value for molecular differentiation of morphologically hardly distinguishable F. hepatica, F. magna and P. cervi eggs after coprological examinations.
A description of karyotype of the giant liver fluke Fascioloides magna (Trematoda, Platyhelminthes) and a review of Fasciolidae cytogenetics
Authors: Reblánová M. , Špakulová M. , Orosová M. , Bazsalovicsová E. , Rajský D. 
Published: May 2010

Abstract
The study describes a karyotype of a common parasite of cervids, the giant liver fluke, Fascioloides magna (Trematoda, Platyhelminthes). The chromosome set of F. magna comprises 11 pairs of chromosomes, all classified as subtelocentric except for the submeta-metacentric pair No. 8 and the submetacentric pair No. 10 (2n = 22, n = 1sm + 1sm-m + 9st). The first longest pair is 4.65 μm long and the length decreases continuously to the 1.92 μm length of the last pair No. 11. No distinct secondary constriction has been observed in mitotic preparations. Fluorescent DAPI-staining reveals distinct heterochromatin bands on all 11 chromosome pairs in the centromeric regions; another DAPI-positive bands are localized at the end of the long arms of chromosomes No. 5 and the last less distinct signals appear interstitially on the long arms of the pair No. 6. Synchronous meiotic divisions of 8-spermatocyte groups have been observed during spermatogenesis, similarly with a development of spermatocytes in other trematodes. In the first two stages of heterotypic spermatocyte division, 11 bivalents (n = 11) are regularly observed, confirming the diploid number of 22 elements. Furthermore, the present analysis summarises and discusses available cytogenetic data on Fasciolidae flukes suitable for future studies on taxonomy or phylogenetic interrelationships within the family.
Diagnosis of Oesophagostomum columbianum infection in goat by indirect enzyme linked immunosorbent assay
Authors: Jas R. , Ghosh J. , Das K. 
Published: May 2010

Abstract
Oesophagostomosis is one of the most prevalent nematodosis with considerable economic losses in small ruminant livestock. Prepatent stage of Oesophagostomum columbianum is primarily involved in the major pathogenic effect of the infection and conventional methods are unable to detect this stage. An indirect enzyme linked immunosorbent assay (ELISA) was therefore standardized and evaluated for the purpose to improve diagnosis and thereafter minimize the economic losses due to this infection in goat. The eggs obtained from O. columbianum adult female worms, collected from caecum and colon of slaughtered goats, were cultured to obtain the third stage larvae (L3). Crude somatic antigen (CSAg) was prepared from triturated worms. Experimental goats (n = 12) were orally infected each with 600 L3 /kg body weight. Serum samples of the infected goats were collected at 2-day-interval from day-3 till day-33 post infection. The indirect ELISA was standardized using the CSAg for coating the wells, infected goat sera as binding antibody, anti-goat IgG-HRP conjugate and ortho-phenylenediamine (OPD) with peroxide as substrate. The sensitivity, specificity and accuracy of ELISA were determined by testing 96 serum samples of goats whose parasitological status after slaughter was determined by examination of the abomasums and the intestines. O. columbianum antibodies were detected in sera of infected goats on day 18 – 27 post-infection with 96.00, 73.91 and 85.42 per cent sensitivity, specificity and accuracy, respectively.
Esophageal deformation of Gnathostoma spinigerum in ivermectin-treated rats, and anthelminthic efficacy
Authors: Anantaphruti M. , Koga M. , Nuamtanong S. , Nintasen R. 
Published: May 2010

Abstract
Ivermectin 2 mg/kg single dose reduced worm load by 83.3 % (75.0 – 91.7 %) for rats experimentally infected with 20 Gnathostoma spinigerum advanced third-stage larvae administered at week 1, with sacrifice at week 15 post-infection. Some larvae recovered from the muscles of treated rats were found to be grossly deformed in appearance. Morphological changes in the Gnathostoma larvae were clearly observed. The internal structures of the deformed larvae showed significant degeneration in the esophageal glandular tissues. The esophageal lumen branched irregularly and occupied a wider area in the center of the esophagus. The epithelial cells thickened along these branches. The esophageal gland ducts were completely destroyed. The excretory ability of the esophageal cell glands may be depressed, causing loss of function. It is suggested that ivermectin is effective against Gnathostoma larvae due to worm recovery reductions. The drug efficacy appears to generate by esophageal destruction of the worms.
Unusual localizations of human hydatid disease in Hamedan province, west of Iran
Authors: Ahmadi N. , Hamidi M. 
Published: May 2010

Abstract
Cystic echinococcosis (CE) is endemic in many parts of Iran. It may develop in almost any part of the human body, though the location is mostly hepatic and followed by pulmonary. This study was carried out on the 24 patients diagnosed with unusual localizations of hydatidosis among the patients admitted to the university hospitals in Hamedan province, west of Iran, during 14-year period. The cases were aged 6 – 73 years, the majority being aged 40 – 59 years (33.3 %). Ten of the patients diagnosed with unusually located hydatid disease were men, fourteen were women. The over-representation of housewives (50.0 %) and followed by farmers (20.8 %) among the cases was significant. Similarly, rural dwellers were also over-represented among the cases (54.2 % vs. 45.8 %).
Most (19 case) of the cases had been diagnosed just by ultrasonography, but 3 case had only been diagnosed by computed tomographic (CT) scans, and another 2 had had both ultrasonography and CT scans. Spleen and kidneys, followed by abdominal/peritoneal cavity, were the organs where hydatid disease was most frequently observed after the liver and lung. The splenic cysts were solitary in 4 cases, associated with a hepatic cyst in 1 and with pelvic and abdominal wall cysts in 1. The renal cysts were usually isolated without any other organ involvement, and there was only one case of associated with hydatid disease in liver. In addition, findings in brain, mesenterium, breast, choledochus, bladder wall and inguinal area are also discussed.
These results confirm that CE should be considered in the differential diagnosis of any cystic mass in all anatomic locations (including unusual localizations), especially when occurring in areas where the disease is endemic.
Endohelminth communities of the perch, Perca fluviatilis (Perciformes, Percidae) from Srebarna Biosphere Reserve, Bulgaria
Authors: Shukerova S. , Kirin D. , Hanzelová V. 
Published: May 2010

Abstract
Altogether 180 specimens of Perca fluviatilis (L.) from Srebarna Lake, North-eastern Bulgaria, were examined for helminths during spring, summer and autumn of 2005 – 2006. Of them, 143 (79.4 %) perch were infected with helminths. Thirteen helminth species were found: metacercariae of Bolbophorus confusus, Diplostomum pseudospathaceum, D. spathaceum, Ichthyocotylurus pileatus, Posthodiplostomum cuticola and Tylodelphys clavata; cestode Proteocephalus percae; acantocephalans Acanthocephalus anguillae and A. lucii and nematode larvae Contracaecum microcephalum, Eustrongilides excisus, E. tubifex and Rhaphidascaris acus. The digenean T. clavata was dominant species of the perch component community. Little seasonal differences were found in the prevalence, abundance and mean infection of helminths. Eustrongilides tubifex reported from the territory of Bulgaria for the first time, represent the new geographical records and new species of Bulgarian helminth fauna.
P. fluviatilis was reported as a new host record for two digeneans, namely Diplostomum pseuspathaceum and Posthodiplostomum cuticola, the cestode Proteocephalus percae, the acantocephalan Acantocephalus anguillae and the nematodes Contracaecum microcephalum and E. tubifex from the territory of Bulgaria. P. fluviatilis was reported as a new host record for D. pseudospathaceum, P. cuticola, C. microcephalum and E. tubifex from Balkan peninsula.
Similar Results in Force-Velocity Test in Disabled Weight Lifters and Able-Bodied Physically Fit Students
Authors: Adach Zdzisław , Jaskólska A. , Jaskólski A. 
Published: May 2010

Similar Results in Force-Velocity Test in Disabled Weight Lifters and Able-Bodied Physically Fit Students
The aim of the study was (1) to evaluate the maximal power using a short-term force-velocity test in disabled weight lifters and able-bodied non-athletes subjects, and (2) to find if the parameters of the force-velocity test are related to results of the weight lifted on a bench, which is a specific sport test among disable weight lifters. The force-velocity (F-V) test was used to evaluate the maximal power (Pmax), predicted maximal resistance force (F0) and velocity (V0). The test was applied to 18 disable weight lifters and 16 physical education students. The mean values of Pmax, V0 and F0 did not differ significantly between groups. In weight lifters, the weight lifted on a bench correlated significantly with Pmax and F0.
It was concluded that (1) in the same F-V test condition (without lower limb support), the results obtained by the able-bodied, non-trained, but physically fit students, do not differ from that of the wheelchair weight lifters; (2) the F-V test is useful for wheelchair weight lifters, as it effectively evaluates their anaerobic power and speed-strength abilities, which is very similar to the able-bodied subjects.
Minnesota Leisure Time Physical Activity Questionnaire as an additional Tool in Clinical Assessment of Patients undergoing Percutaneous Coronary Interventions
Authors: Nowak Zbigniew , Plewa Michal , Skowron Malgorzata , Osiadlo Grażyna , Markiewicz Andrzej , Kucio Cezary 
Published: May 2010

Minnesota Leisure Time Physical Activity Questionnaire as an additional Tool in Clinical Assessment of Patients undergoing Percutaneous Coronary Interventions
The aim was to analyze the usability of Minnesota Leisure Time Physical Activity Questionnaire (MLTPAQ) in assessment of physical activity in patients before and 6 months after percutaneous coronary intervention (PCI). The study group consisted of 211 patients aged between 34-79 years (x = 59,5±7,89 yeras), with history of ischemic heart disease (IHD) with or without previous incidence of myocardial infarction (MI). The MLTPAQ was administered to all patients at the time of PCI and then 6 months later, as was the treadmill stress test (TST) and echocardiography (ECHO). Total energy expenditure calculated with the MLTPAQ remained at the same level and was of low intensity (<4 MET, <2000kcal/week) 6 months after the PCI. There was an increased physical capacity noted 6 months after initial PCI: increased metabolic cost (MET); maximal oxygen uptake (VO2max); maximal heart rate (HRmax) obtained during the TST and decreased resting heart rate (HRrest). ECHO examination showed improved LVEF%. Despite increased physical capacity and improved heart hemodynamics, resulting most likely from PCI procedure, the patients showed a similar level of leisure time physical activity 6 months after the PCI.
Title IX Compliance in NCAA Athletic Departments: Perceptions of Senior Woman Administrators
Authors: Schneider Robert , Stier William , Henry Timothy , Wilding Gregory 
Published: May 2010

Title IX Compliance in NCAA Athletic Departments: Perceptions of Senior Woman Administrators
Perceptions of Senior Woman Administrators (SWAs) were sought regarding the equal provision of 13 Title IX compliance areas in women's athletic programs as compared to men's. A five point agree/disagree Likert-scale survey was electronically mailed to all SWAs at National Collegiate Athletic Association (NCAA) membership institutions throughout the United States. Of the 841 surveys mailed, 406 were returned for a 48.3% return rate. The SWAs disagreed or strongly disagreed at the highest rates that the following five Title IX compliance areas were being provided for equally in the women's programs when compared to the men's: publicity (31.0%), locker room facilities (27.1%), coaching (20.0%), recruitment of student-athletes (15.4%), and equipment and supplies (14.7%). Significant differences between Likert-scale items of agreement/disagreement were found among the following SWA demographics: marital status, NCAA Division, years of experience, and reporting structure. The SWAs agreed or strongly agreed that the 13 Title IX compliance areas were being provided for equally in the women's programs when compared to the men's at the following rates: housing & dining facilities (84.4%), medical & training facilities (84.3%), scheduling of games (81.1%), travel & per diem allowances (80.5%), practice facilities (78.7%), competitive facilities (78.4%), equipment and supplies (77.7%), support services (76.0%), tutoring (74.3%), recruitment of student-athletes (73.2%), coaching (70.3%), locker room facilities (63.2%), and publicity (55.3%).