bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immune- and metabolism-related gene signature analysis uncovers the prognostic and immune microenvironments of hepatocellular carcinoma
Authors: Gu Yange , Ma Ensi , Jiang Shengran , Shan Zhenyu , Xia Guixi , Ma Rui , Fu Jiaqi , Wang Zhengxin 
Published: June 2024

Abstract
Background
Metabolic reprogramming is an emerging hallmark that influences the tumour microenvironment (TME) by regulating the behavior of cancer cells and immune cells. The relationship between metabolism and immunity remains elusive. The purpose of this study was to explore the predictive value of immune- and metabolism-related genes in hepatocellular carcinoma (HCC) and their intricate interplay with TME.

Methods
We established the immune- and metabolism-related signature (IMRPS) based on the LIHC cohort from The Cancer Genome Atlas (TCGA) dataset. Kaplan–Meier analysis, receiver operating characteristic (ROC) curve analysis and Cox regression analysis confirmed the prognostic value of IMRPS. We investigated differences in immune cell infiltration, clinical features, and therapeutic response between risk groups. The quantitative real-time PCR (qPCR) was used to confirm the expression of signature genes. Immunohistochemical staining was performed to evaluate immune infiltration features in HCC tissue samples. We conducted cell experiments including gene knockout, cell counting kit-8 (CCK-8), and flow cytometry to explore the role of the IMRPS key gene UCK2 in HCC. RNA-seq was used to further investigate the potential underlying mechanism involved.

Results
The IMRPS, composed of four genes, SMS, UCK2, PFKFB4 and MAPT, exhibited significant correlations with survival, immune cell infiltration, clinical features, immune checkpoints and therapeutic response. The IMRPS was shown to be an excellent predictor of HCC prognosis. It could stratify patients appropriately and characterize the TME accurately. The high-risk HCC group exhibited an immunosuppressive microenvironment with abundant M2-like macrophage infiltration, which was confirmed by the immunohistochemistry results. The results of qPCR revealed that the expression of signature genes in 20 HCC tissues was significantly greater than that in adjacent normal tissues. After the key gene UCK2 was knocked out, the proliferation of the Huh7 cell line was significantly inhibited, and monocyte-derived macrophages polarized towards an M1-like phenotype in the coculture system. RNA-seq and GSEA suggested that the phenotypes were closely related to the negative regulation of growth and regulation of macrophage chemotaxis.

Conclusions
This study established a new IMRS for the accurate prediction of patient prognosis and the TME, which is also helpful for identifying new targets for the treatment of HCC.
Patterns of acute hospital and specialist palliative care use among people with non-curative upper gastrointestinal cancer
Authors: Boland E. G. , Tay K. T. , Khamis A. , Murtagh F. E. M. 
Published: June 2024

Abstract
Purpose
Upper gastrointestinal (GI) cancers contribute to 16.7% of UK cancer deaths. These patients make high use of acute hospital services, but detail about palliative care use is lacking. We aimed to determine the patterns of use of acute hospital and hospital specialist palliative care services in patients with advanced non-curative upper GI cancer.

Methods
We conducted a service evaluation of hospital use and palliative care for all patients with non-curative upper GI cancer seen in one large hospital, using routinely collected data (2019–2022). We report and characterise hospital admissions and palliative care within the study time period, using descriptive statistics, and multivariable Poisson regression to estimate the unadjusted and adjusted incidence rate ratio of hospital admissions.

Results
The total with non-curative upper GI cancer was 960. 86.7% had at least one hospital admission, with 1239 admissions in total. Patients had a higher risk of admission to hospital if: aged ≤ 65 (IRR for 66–75 years 0.71, IRR 76–85 years 0.68; IRR > 85 years 0.53; p < 0.05), or lived in an area of lower socioeconomic status (IMD Deciles 1–5) (IRR 0.90; p < 0.05). Over the 4-year period, the rate of re-admission was higher in patients not referred to palliative care (rate 0.52 readmissions/patient versus rate 1.47 readmissions/patient).

Conclusion
People with advanced non-curative gastrointestinal cancer have frequent hospital admissions, especially if younger or from areas of lower socioeconomic status. There is clear association between specialist palliative care referral and reduced risk of hospitalisation. This evidence supports referral to specialist palliative care.
The effectiveness of reminiscence therapy on the symptom management, the life satisfaction, and the self-transcendence in palliative care patients: a randomized controlled trial
Authors: Bozkurt Canan , Yildirim Yasemin 
Published: June 2024

Abstract
Purpose
To examine the effect of individualized reminiscence therapy on the management of global distress and physical and psychological symptoms, life satisfaction and self-transcendence levels of palliative care patients.

Methods
In a single-center palliative care service in western Turkey, 48 patients without cognitive impairment and able to communicate were included in the study. However, 44 patients completed the study. Patients who met the inclusion criteria were randomly assigned to the reminiscence therapy (intervention), unstructured social interviewing (placebo), and control groups (16 people for each group) before the start of the study. The sessions for the interview and placebo groups were conducted face-to-face in the patient's room (while the patient was sitting or lying down) for 15 days (2 weeks), every other day, for a total of eight sessions (each session was approximately 30 min). Data collection instruments—the Memorial Symptom Assessment Scale, the Contentment with Life Assessment Scale, and the Self-Transcendence Scale—were collected at baseline (first day) and after the intervention (day 15th). Statistical significance level was accepted as p < 0.05.

Results
There was no decrease in physical and total symptom burden (p > 0.05). There were significant reductions in general distress and psychological symptoms in the intervention and placebo groups within the group (p < 0.05), but there were no significant differences between the control group and all groups when compared (p > 0.05). Group × time interactions were statistically significant for life satisfaction and self-transcendence (p < 0.001), and there was a substantial increase in the intervention group compared to the other groups.

Conclusion
It may be recommended that reminiscence therapy intervention be included in routine nursing care as it may contribute positively to the psychological recovery of palliative care patients approaching the end of life.

Trial registration
ClinicalTrails.gov (Registration number: NCT05242016). Prospectively registered on 1 February 2022.
Endoscopic transcavernous approach for functional pituitary adenomas
Authors: Starnoni Daniele , Daniel Roy T. , Messerer Mahmoud 
Published: June 2024

Abstract

Background
Invasion of the CS is one of the limiting factors for total resection for PitNet tumors with cure rates less than 30%. Extended approaches may be considered in selective and well-studied cases of secreting adenomas.

Method
We describe the key steps of the endoscopic transcavernous approach for functional pituitary adenomas with a video illustration. The surgical anatomy is described along with the advantages and limitations of this approach.

Conclusion
A detailed knowledge of CS anatomy and familiarity with this surgical approach acquired in the laboratory is essential. Proper instrumentation is critical to decrease the risks of vascular injury.
Denial of reproductive potential: a predictor of unperceived pregnancy in an Austrian neonaticide sample
Authors: Klier Claudia M. , Ina Bozic , Kuipers Yvonne , Amon Sabine 
Published: June 2024

Abstract
Purpose
This study aims to describe the phenomenon of unperceived pregnancy followed by neonaticide with a focus on the lack of awareness of reproductive potential in an Austrian sample.

Methods
An explorative comparative study of neonaticide cases with single and repeat perpetrators was conducted using nationwide register-based data from 1995 to 2017. A total number of 55 cases out of 66 were included in the analysis. A standardized coding sheet was used and calculations were performed.

Results
48 women gave birth to 101 children, of which 55 were killed, 23 children lived out of home care and 23 lived with the perpetrator We found a higher fertility rate in both neonaticide perpetrators in the single (1,9) and the repeat group (4,25) in comparison to the general population (1,4). The use of contraception was only 31% among neonaticide perpetrators, deviating substantially from the general Austrian population age group (16-29yrs) which used contraception in 91%. The neonaticide perpetrators used an effective contraception method (pearl-index < 4) in only 2%, whereas 20% of the general population did so. The number of unperceived pregnancies was high in both groups (50/55) 91%.

Conclusion
Future case reports and forensic evaluations should take reproductive behavior into account, as it may offer valuable insights into the events leading up to neonaticide. Our findings suggest that denial of reproductive potential often precedes unperceived pregnancies. In the Austrian cohort, women who experienced unperceived pregnancies resulting in unassisted births and subsequent neonaticide showed a low prevalence of contraceptive use. This is particularly noteworthy given that the primary motive for neonaticide is unwanted pregnancy.
Beginnings and endings—dance phrase edges in an interactive dance study
Authors: Bergsland Andreas 
Published: June 2024

AbstractThis article describes a study exploring the expressive and creative potential of dance phrase onsets and endings in interactive dance, using an artistic research approach. After describing the context and background of interactive dance and relevant perceptual issues, the technical setup is presented, both in terms of the performance area, sensors and other hardware, and software. The main part of the article is concerned with the specific movement-sound mappings and their relation to the dancer’s movement choices in four different sections in the performance that the project resulted in, entitled Beginnings and Endings - Study I. Subsequently, the process and performance are evaluated, specifically focusing on the performer’s perspective. The most interesting points from the evaluation are then discussed with reference to the presented mappings, related movement choices, and relevant research literature. Findings include that the dancer experienced an increased awareness of beginnings and endings in different sections of the performance, that the affordances of different mappings were explored in different ways and to different degrees, and that some of this could be ascribed to a dynamic between liberation and restriction.
Causal analysis of positive Reaction Systems
Authors: Brodo Linda , Bruni Roberto , Falaschi Moreno , Gori Roberta , Milazzo Paolo , Montagna Valeria , Pulieri Pasquale 
Published: June 2024

AbstractCause/effect analysis of complex systems is instrumental in better understanding many natural phenomena. Moreover, formal analysis requires the availability of suitable abstract computational models that somehow preserve the features of interest. Our contribution focuses on the analysis of Reaction Systems (RSs), a qualitative computational formalism inspired by biochemical reactions in living cells. The primary challenge lies in dealing with inhibition mechanisms. On the one hand, inhibitors enhance the expressiveness of the computational abstraction; on the other hand, they can introduce nonmonotonic behaviors that can be computationally hard to deal with in the analysis. We propose an encoding of RSs into an equivalent formulation without inhibitors (called Positive RSs, PRSs for short) that is easier to handle, because PRSs exhibit monotonic behaviors. The effectiveness of our transformation is witnessed by its impact on two different techniques for cause/effect analysis. The first, called slicing, allows detecting the causes of some unforeseen phenomenon by reasoning backward along a given computation. Here, PRSs can be exploited to improve the quality of the analysis. The second technique, predictor analysis, is addressed by introducing a novel tool called MuMa, which is based on must/maybe sets, whence the tool name, an original abstraction for approximating ancestor formulas. MuMa exploits PRSs to improve the performance of the analysis.
Cruroplasty as a standalone treatment for recurrent hiatal hernia repair
Authors: Tran Ashley , Putnam Luke R. , Harvey Lucy , Lipham John C. 
Published: June 2024

Abstract
Purpose
Following laparoscopic anti-reflux surgery (LARS), recurrence of hiatal hernia is common. Patients with symptomatic recurrence typically undergo revision of the fundoplication or conversion to magnetic sphincter augmentation (MSA) in addition to cruroplasty. However, patients with an intact fundoplication or MSA may only require repeat cruroplasty to repair their recurrent hiatal hernia. The purpose of this study is to compare outcomes following cruroplasty alone compared to full revision (i.e. redo fundoplication or MSA with cruroplasty) for the management of recurrent hiatal hernias.

Methods
A retrospective review of patients undergoing surgical revision of a symptomatic recurrent hiatal hernia between February 2009 and October 2022 was performed. Preoperative characteristics, intraoperative details, and postoperative outcomes were compared between patients undergoing cruroplasty alone versus full revision.

Results
A total of 141 patients were included in the analysis. 93 patients underwent full revision, and 48 patients underwent cruroplasty alone. The mean time between initial and revisional surgery was 8 ± 7.7 years. There was no significant difference in operative time or rates of intra-operative or post-operative complication between groups. Patients undergoing cruroplasty alone had a mean Gastroesophageal Reflux Disease Health Related Quality Life (GERD-HRQL) Questionnaire score of 9.6 ± 10.2 compared to a mean score of 8.9 ± 11.2 for full revision patients (p = 0.829). Recurrence rates following revision was 10.4% for cruroplasty alone patients and 11.8% in full revision patients (p > 0.999).

Conclusion
In patients with intact fundoplication or MSA, cruroplasty alone results in similar post-operative outcomes compared to full revision for recurrent hiatal hernia.
Phantoms for Quantitative Body MRI: a review and discussion of the phantom value
Authors: Keenan Kathryn E. , Jordanova Kalina V. , Ogier Stephen E. , Tamada Daiki , Bruhwiler Natalie , Starekova Jitka , Riek Jon , McCracken Paul J. , Hernando Diego 
Published: June 2024

AbstractIn this paper, we review the value of phantoms for body MRI in the context of their uses for quantitative MRI methods research, clinical trials, and clinical imaging. Certain uses of phantoms are common throughout the body MRI community, including measuring bias, assessing reproducibility, and training. In addition to these uses, phantoms in body MRI methods research are used for novel methods development and the design of motion compensation and mitigation techniques. For clinical trials, phantoms are an essential part of quality management strategies, facilitating the conduct of ethically sound, reliable, and regulatorily compliant clinical research of both novel MRI methods and therapeutic agents. In the clinic, phantoms are used for development of protocols, mitigation of cost, quality control, and radiotherapy. We briefly review phantoms developed for quantitative body MRI, and finally, we review open questions regarding the most effective use of a phantom for body MRI.
Unmasking Individual and Institutional HIV Stigma in Hospitals: Perspectives of Dutch Healthcare Providers
Authors: Jordans C. C. E. , Vliegenthart-Jongbloed K. J. , van Bruggen A. W. , van Holten N. , van Beek J. E. A. , Vriesde M. , van der Sluis D. , Verbon A. , Roukens A. H. E. , Stutterheim S. E. , Rokx C. 
Published: June 2024

AbstractPeople with HIV continue to experience HIV stigma. Quantitative data on HIV stigma perpetrated by healthcare providers of hospitals providing HIV care in high-income countries are limited. The aim of this study is to investigate factors associated with HIV stigma in Dutch healthcare settings from the healthcare providers’ perspective. We conducted a cross-sectional study using the questionnaire ‘Measuring HIV Stigma and Discrimination Among Health Facility Staff – Monitoring Tool for Global Indicators’ to assess HIV stigma among healthcare providers (n = 405) in two academic hospitals. Healthcare providers licensed to provide medical care were eligible for inclusion. The primary outcome was the self-reported prevalence of at least one manifestation of HIV stigma measured by six stigma indicators (four individual, two institutional). Secondary outcomes were the prevalence of HIV stigma per indicator, per occupation, per department, and factors associated with individual stigma indicators. HIV stigma was prevalent among 88.1% (95%CI 84.5% − 91.2%) of participants. Stigma was mostly driven by negative attitudes towards people with HIV and worry to acquire HIV. Multivariate analysis showed that several factors were associated with HIV stigma, including younger age, male sex, working at one of the surgical departments, and working as a nurse. Having received any training on HIV stigma and/or discrimination was associated with less HIV stigma among all indicators. In conclusion, HIV stigma is highly prevalent among Dutch healthcare providers. Targeted approaches, including training on HIV stigma and discrimination, are needed to reduce HIV stigma in healthcare and should, among others, focus on younger healthcare providers.
The Associations Between Attachment Insecurity and Compulsive Sexual Behavior Disorder or Problematic Pornography Use: The Mediating Role of Emotion Regulation Difficulties
Authors: Wizła Magdalena , Lewczuk Karol 
Published: June 2024

AbstractCompulsive sexual behavior disorder (CSBD) was previously considered an attachment disorder, while emotion dysregulation was thought to potentially be a key characteristic of it. However, this theoretical model was not tested in previous empirical research. In our cross-sectional study, we tested whether emotional regulation (ER) difficulties can be adopted as an explanatory mechanism for the relationships between attachment avoidance and anxiety, as well as CSBD and its most prevalent behavioral presentation—problematic pornography use (PPU). Participants (n = 1002; Mage = 50.49 years, SD = 13.32; men: 50.2%) completed an online survey regarding the investigated variables. In mediation analyses, attachment avoidance and anxiety were treated as simultaneous predictors, ER difficulties as a mediating variable, with CSBD/PPU severity as dependent variables. Emotion regulation difficulties and attachment anxiety had a direct positive effect on both CSBD and PPU. The direct effect of attachment avoidance on PPU was non-significant, and significant for CSBD depending on the measure used. Moreover, all the relationships between both insecure attachment dimensions and CSBD/PPU symptom severity were at least partially mediated by ER difficulties. Our results corroborate the theoretical claim that ER difficulties may be a useful framework for explaining the impact of attachment insecurity on CSBD/PPU. Theoretical and practical implications of the findings are discussed.
BET-directed PROTACs in triple negative breast cancer cell lines MDA-MB-231 and MDA-MB-436
Authors: Teufelsbauer Maryana , Stickler Sandra , Eggerstorfer Marie-Therese , Hammond Dennis Clyde , Hamilton Gerhard 
Published: June 2024

Abstract
Purpose
This study aims to find whether the proliferation and migration of triple negative breast cancer (TNBC) cell lines can be reduced by treatment with bromodomain and extra-terminal domain (BET) inhibitor JQ1 and BET protein targeting chimeras (PROTACs) ARV-771 and MZ1.

Methods
Cytotoxicity tests, scratch migration assays and western blot proteome profiler arrays for protein expression of cancer-related proteins were used to evaluate the impact of a BET-inhibitor and two BET-directed PROTACs on cell viability, migration and on protein expression.

Results
JQ1 and the PROTACs MZ1 and ARV-771 significantly inhibited the growth and migration of the KRAS G13D-mutated MDA-MB-231 cells. In this cell line, the PROTACs suppressed the residual expression of ERBB2/HER2, 3 and 4 that are essential for the proliferation of breast cancer cells and this cell line proved sensitive to HER2 inhibitors. In contrast, the effects of the PROTACs on the protein expression of MDA-MB-436 cells mostly affected cytokines and their cognate receptors.

Conclusion
The degradation of BET-protein by PROTACs demonstrated significant anti-proliferative effects. The KRAS-mutated MDA-MB-231 cells belong to the low-HER2 expressing tumors that have a poorer prognosis compared to HER2-null patients. Since first oral PROTACs against tumor hormone receptors are in clinical trials, this mode of tumor therapy is expected to become an important therapeutic strategy in the future treatment of TNBC.
Negotiating becoming: a Nietzschean critique of large language models
Authors: Fischer Simon W. S. , Boer Bas de 
Published: June 2024

AbstractLarge language models (LLMs) structure the linguistic landscape by reflecting certain beliefs and assumptions. In this paper, we address the risk of people unthinkingly adopting and being determined by the values or worldviews embedded in LLMs. We provide a Nietzschean critique of LLMs and, based on the concept of will to power, consider LLMs as will-to-power organisations. This allows us to conceptualise the interaction between self and LLMs as power struggles, which we understand as negotiation. Currently, the invisibility and incomprehensibility of LLMs make it difficult, if not impossible, to engage in such negotiations. This bears the danger that LLMs make reality increasingly homogeneous by recycling beliefs and creating feedback loops that ultimately freeze power struggles and thus consolidate the status quo. In view of this, LLMs constrain self-formation. Based on our critique, we provide some recommendations on how to develop interactions with LLMs that enable negotiations that allow for different ways of being
Synthesis and characterization of copper tin sulfide counter electrode for enhanced performance in third-generation solar cells
Authors: Salunke Supriya A. , Shelke Harshad D. , Mujawar Almas , Bhujbal Pankaj K. , Shaikh Shoyebmohamad F. , Pathan Habib M. , Kowshik Suhas , Naik Nithesh 
Published: June 2024

AbstractThis paper presents the fabrication of a copper tin sulfide (CTS) counter electrode for application in third-generation solar cells. The fabrication process involved modified chemical bath deposition (M-CBD) or a successive ionic layer adsorption reaction (SILAR). Initially, a ZnO seed layer was deposited onto a fluorine-doped tin oxide (FTO) substrate via CBD. Subsequently, a mesoporous layer of ZnO was deposited onto the FTO substrate using the doctor-blade method. The mesoporous ZnO layer was sensitized with CdS nanocrystals deposited using the SILAR method. Various characterization techniques, including UV–Vis spectroscopy, X-ray diffraction (XRD), and scanning electron microscopy (SEM), were employed to analyze the optical, structural, and morphological properties of the photoanode and CTS counter electrode. The results demonstrate the successful synthesis of a CTS counter electrode with desirable properties for solar-cell applications. The fabricated CTS counter electrode exhibited a conversion efficiency of 0.49%, which was significantly higher than that of carbon-based counter electrodes.