bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extensive oral shedding of human herpesvirus 8 in a renal allograft recipient
Authors: Al‐Otaibi L. M. , Al‐Sulaiman M. H. , Teo C. G. , Porter S. R. 
Published: August 2010

Introduction:  Studies were conducted to investigate changes in the extent of human herpesvirus 8 (HHV‐8) shedding and diversity of HHV‐8 strains in the mouth of a renal allograft recipient who developed cutaneous post‐transplantation Kaposi’s sarcoma.Methods:  Matched oral and blood samples were obtained from a Saudi Arabian renal allograft recipient from 3 days before to 38 weeks after transplantation, and from his kidney donor. Polymerase chain reaction (PCR) protocols to amplify selected HHV‐8 sub‐genomic regions were applied to detect and quantify HHV‐8 DNA. Sequence diversity was determined by cloning the PCR products and subjecting them to denaturing gradient gel electrophoresis and to nucleotide sequencing.Results:  Before transplantation, the recipient was seropositive for anti‐HHV‐8 immunoglobulin G, but the donor was seronegative; HHV‐8 DNA could be detected in the recipient’s blood, whole‐mouth saliva (WMS) and buccal exfoliates, and the salivary viral load was estimated as 2.6 million genome‐copies/ml. Post‐transplantation, the recipient’s salivary viral load initially increased to 4.1 million genome‐copies/ml, and thereafter declined precipitously, coinciding with an increase in the dosage of valaciclovir given; HHV‐8 DNA was detected most often in WMS compared with parotid saliva, and buccal and palatal exfoliates. Carriage of multiple HHV‐8 strains was evident in blood and oral samples; whereas before transplantation strains belonging to genotypes A1 and A5 were observed, after transplantation genotype A5 strains became dominant and A2 strains emerged.Conclusion:  Immunosuppression and antiviral prophylaxis may interact to influence the spectrum of oral HHV‐8 strains and the extent of post‐transplantation HHV‐8 shedding into the mouth.
Detection of a single bacterial cell using a 16S ribosomal RNA‐specific oligonucleotide probe designed to investigate periodontal pathogens
Authors: Tsuruda K. , Shimazu A. , Sugai M. 
Published: August 2010

Introduction: The current detection methods for periodontopathogens mainly use polymerase chain reactions. However, there are few methods available for visualizing the bacteria that impact on patients with periodontal disease for use in health education. The purpose of this study was to develop a specific detection method to visualize periodontopathogenic bacteria.Methods: Fluorescently‐labeled oligonucleotide probes directed to specific 16S ribosomal RNA (rRNA) sequences ofPorphyromonas gingivalisandAggregatibacter actinomycetemcomitanswere synthesized. Cultured individual bacterial species were fixed with 4% paraformaldehyde and smeared on glass slides. Fluorescein isothiocyanate‐labeled oligonucleotide probes were hybridized under stringent conditions with smeared whole cells, and then probe specificity was investigated by epifluorescence microscopy.Results: Comparatively long (50‐mer) oligonucleotide probes forP. gingivalisandA. actinomycetemcomitanswere designed. These probes clearly hybridized with 16S rRNA of the target speciesin situand single bacterial cells were detectable visually. The probes exhibited no cross‐hybridization against the additional organisms that were closely related to the target species.Conclusions: The fluorescencein situhybridization technique is a specific and reliable method by which to visually identify the target organisms. The oligonucleotide probes designed in this study will be useful for detectingP. gingivalisandA. actinomycetemcomitanspopulations.
Haemolytic Disease of the Newborn due to Anti‐Dia and Incidence of the Dia Antigen in Poland
Authors: Kuśnierz‐Alejska Grażyna , Bochenek Stanislawa 
Published: August 2010

AbstractA strong complement‐fixing incomplete anti‐Dia was found in the serum of a Polish mother who gave birth to a newborn suffering from a severe haemolytic anaemia. The whole family was of Polish origin. 9,661 donor blood samples from different regions of Poland were tested against antiserum derived from that mother. Dia antigen was present on red cells of 45 (0.46%) individuals. All of them were of Polish origin. In some cases, family studies were undertaken.
Report on the 13th International Society of Blood Transfusion Platelet Immunology Workshop
Authors: Foxcroft Z. , Campbell K. , Merieux Y. , Urbaniak S. , Brierley M. , Rigal D. , Ouwehand W. H. , Metcalfe P. 
Published: August 2010

Background and Objectives  The aim of the 13th International Society of Blood Transfusion Platelet Immunology Workshop was to compare the sensitivity and specificity of the in‐house method for the detection of human platelet antigen (HPA) antibodies currently used in participating laboratories with a modified rapid protocol for the monoclonal antibody (mAb) immobilization of platelet antigen (MR‐MAIPA) assay.Materials and Methods  Twenty‐eight laboratories from 15 countries participated. A set of four freeze‐dried minimum potency reference reagents with known single‐specificity HPA antibodies were supplied for testing by titration with both assays and two coded freeze‐dried plasma samples were provided for antibody specificity testing. Critical reagents and materials for the MR‐MAIPA were provided including lyophilized panel platelets and five capture mAbs.Results  Titration of the reference standards showed that the sensitivity of the MR‐MAIPA was the same as the in‐house methods. The proposed replacement anti‐HPA‐1a reference reagent 05/106 gave results that did not differ significantly from the current reference reagent 93/710. The results with the two blinded samples showed that in the first sample, 27 out of the 28 laboratories were able to correctly identify the anti‐HPA‐1a present when using their respective in‐house methods, but only 23 correctly identified the antibody when using the rapid MAIPA method. The results from the second sample, which contained multispecificities, showed that only 50% of the participants correctly identified all five antibodies present using their in‐house method. The results for the rapid MAIPA were lower, with only 32% identifying all specificities. The variability in the reconstitution of the freeze‐dried platelets may have been one of the contributing factors to the poorer results.Conclusions  The sensitivity of the MR‐MAIPA compared favourably with that of the in‐house methods. Most laboratories were able to identify anti‐HPA‐1a alone in Sample 1 but more than half of the participants were not able to correctly assign the specificity of all HPA antibodies present in the second sample. The usefulness of the panel of freeze‐dried platelets varied considerably between laboratories.
Severe fetomaternal alloimmune thrombocytopenia due to anti‐human platelet antigen (HPA)‐1a in a mother with a rare and silenced ITGB3*0101 (GPIIIa) allele
Authors: Smith G. A. , Rankin A. , Riddle C. , Cheetham‐Wilkinson C. , Ranasinghe E. , Ouwehand W. H. , Watkins N. A. 
Published: August 2010

Background and Objectives  Fetomaternal alloimmune thrombocytopenia (FMAIT) is caused by maternal antibodies against a human platelet antigen (HPA) present on fetal, but absent from maternal platelets. We identified and characterized a case of FMAIT due to anti‐HPA‐1a in a mother with an HPA‐1a1b genotype.Materials and Methods  The first child of a 29‐year‐old mother presented with a petechial rash and a platelet count of 8 × 109 per l. Upon routine serological investigation, a discrepancy between the HPA‐1a genotype and phenotype prompted the sequencing of the 15 exons of the ITGB3 (integrin β3, GPIIIa and CD61) gene in the mother.Results  The mother was genotypically HPA‐1a1b heterozygous but phenotyped as HPA‐1a negative. Sequencing of the ITGB3 exons confirmed HPA‐1a1b heterozygosity, but also identified a novel single nucleotide insertion in exon 10 leading to a frameshift and premature termination at amino acid 471 of ITGB3. Maternal anti‐HPA‐1a was detected but with a pattern typical for a low‐affinity antibody. Three transfusions of HPA‐1a and ‐5b negative neonatal platelet concentrates were required to return to a safe platelet count.Conclusion  A rare ITGB3 allele was uncovered by the investigation of a severe case of alloimmune thrombocytopenia in a mother with HPA‐1a antibodies who genotyped as HPA‐1a1b.
Investigation of the thrombin‐generating capacity, evaluated by thrombogram, and clot formation evaluated by thrombelastography of platelets stored in the blood bank for up to 7 days
Authors: Johansson P. I. , Svendsen M. S. , Salado J. , Bochsen L. , Kristensen A. T. 
Published: August 2010

Background and Objectives  Transfusion based on the Thrombelastograph (TEG®) results reduces transfusion requirements in cardiac surgery and in liver transplantation. Taking the pivotal role of thrombin generation in the coagulation process into consideration, the clinical utility of the TEG may, in part, depend on its reflection of the dynamics of thrombin generation.Material and Methods  The kinetics of thrombin generation of platelets stored for 2 and 7 days, respectively, was assessed by calibrated automated thrombogram (CAT) and the lag time (min), time to peak (ttPeak; min), peak (nm thrombin) and endogenous thrombin potential (ETP; nm thrombin*min) were registered. Clot formation was evaluated by TEG and the R time (min), maxial amplitude (MA; mm), time to maximum thrombus generation (TMG; min) and maximum thrombus generation (MTG; dynes cm−2 s−1) and total thrombus generation (TTG; dyne cm−2) were registered.Results  Platelets become more procoagulant, evaluated both by TEG and CAT during storage. The reduction in CAT lag time and the ttPeak correlated with a decrease in the TEG R time and TMG (P < 0·0001) as did the CAT peak thrombin generation and the TEG MTG (P = 0·0035). No correlation between ETP and TTG was found (P = 0·65).Conclusion  The kinetics of thrombin generation, as evaluated by CAT, correlates with the thrombus generation, as evaluated by thrombelastography and this may in part explain the clinical utility of the TEG in identifying clinically relevant coagulopathies, secondary to impaired thrombin generation.
Effect of the antimicrobial peptide indolicidin on the green peach aphid Myzus persicae (Sulzer)
Authors: Le‐Feuvre R. R. , Ramírez C. C. , Olea N. , Meza‐Basso L. 
Published: August 2010

Abstract:  The green‐peach aphid, Myzus persicae (Sulzer) (Hem., Aphididae), is a major agricultural pest of a wide range of host plants, causing damage by feeding and indirectly by transmitting viruses. In this study we tested the effect of the antimicrobial peptide indolicidin on M. persicae survival and on its essential bacterial endosymbionts. Artificial diet bioassays showed a significant dose‐dependent lethal response of indolicidin on M. persicae survival (LD50 of 209 ± 60 μg/ml). Histological analysis of indolicidin‐treated aphids revealed a lower number of distorted mycetocytes, whereas control aphids showed abundant number of rounded and filled mycetocytes. These results suggest that aphid survival could be affected via reduction of its endosymbionts. Thus, aphid control based on antimicrobial substances against endosymbionts could be a promising strategy that needs to be further explored.
Oviposition deterrence of shoots and essential oils of Minthostachys spp. (Lamiaceae) against the potato tuber moth
Authors: Guerra P. C. , Molina I. Y. , Yábar E. , Gianoli E. 
Published: August 2010

Abstract:  The potato tuber moth is a noxious pest of potato in stores, where the use of repellent plants is an environmentally sound alternative to the application of chemical pesticides. We evaluated the protective effect of native Minthostachys species (Lamiaceae) against tuber infestation by the potato tuber moth in a rustic store in Cusco, Perú. We covered potato tubers with dried shoots of Minthostachys spicata and Minthostachys glabrescens and compared tuber damage with a control treatment of maize straw. We also conducted a no‐choice oviposition bioassay in the laboratory, testing the oviposition deterrence of essential oils of M. spicata, M. glabrescens and Minthostachys mollis at natural concentrations. We recorded the number of eggs laid by mated moths on filter paper treated with essential oils of each of the three species and on two control treatments: hexane and untreated blank. Finally, we tested for differences in oviposition deterrence among five full‐sib families of potato tuber moth raised under identical conditions. We found that dried, chopped leaves and flowers of Minthostachys species reduced the percentage of tuber damage in stores in comparison with the control (5% vs. 12%), but no difference in protection was found between species. Essential oils at natural concentrations deterred moth oviposition, reducing the number of eggs laid by about 80% compared with the control treatments; again, there were no significant differences between Minthostachys species. Finally, whereas we detected among‐family variation in oviposition on filter papers treated with essential oils, no difference was found in the number of eggs laid on control substrates. Therefore, there was genetic variation for oviposition deterrence in the potato tuber moth and resistance to repellent plants might evolve thereafter.
Millennium‐scale recurrent uplift inferred from beach deposits bordering the eastern Nankai Trough, Omaezaki area, central Japan
Authors: Fujiwara Osamu , Hirakawa Kazuomi , Irizuki Toshiaki , Hasegawa Shiro , Hase Yoshitaka , Uchida Jun‐ichi , Abe Kohei 
Published: August 2010

AbstractA flight of Holocene marine terraces on the southwestern coast of Cape Omaezaki of central Japan provides evidence of recurrent millennium‐scale uplift events. We reconstructed the uplift history of these terraces by using facies analysis of drill core and geoslicer samples, environmental analysis of trace fossils, and 14C age determinations. Coastal uplift can be identified by the displacement of beach deposits such as foreshore deposits, which represent the intertidal swash zone of a wave‐dominated sandy coast. Three levels of former beach deposits facing the Nankai Trough were identified near the coast in the Omaezaki area. The highest of these, dated at about 3020–2880 BC, records a maximum of 2.2–2.7 m of emergence. The middle beach surface, of minimum age 370–190 BC, shows 1.6–2.8 m of emergence. The lowest beach surface, which is older than 1300–1370 AD, records 0.4–1.6 m of emergence. Our analysis of vertical crustal deformation data during the Holocene in this region suggests that rapid and strong uplift was restricted to the southwestern coast of the Omaezaki area and was probably caused by high‐angle thrusting on subsidiary faults branching from the underlying plate boundary megathrust.
Detailed measurements of thickness and grain size of a widespread onshore tsunami deposit in Phang‐nga Province, southwestern Thailand
Authors: Fujino Shigehiro , Naruse Hajime , Matsumoto Dan , Sakakura Norihiko , Suphawajruksakul Apichart , Jarupongsakul Thanawat 
Published: August 2010

AbstractMeasurements of thickness and grain size along flow‐parallel transects across onshore deposits of the 2004 Indian Ocean tsunami revealed macroscopic horizontal variations and provided new insights into tsunami sedimentation. The tsunami caused severe erosion of beaches, river mouths, and the shallow seafloor along the coast of southwestern Thailand and supplied sufficient sediment to deposit a kilometer‐wide blanket of sand on the land surface. The tsunami deposits generally fine landward with some fluctuations caused by local entrainment and settlement of sediments. Sediments of medium and fine sand are restricted to a few hundreds of meters inland from their source, whereas finer grained sediments were suspended longer and deposited 1 km or more inland. Although the thickness of the tsunami deposits is strongly influenced by local topography, they generally thin landward. In areas of low‐relief topography, the rate of landward thinning is exponential and reflects the dominance of sediment supply to nearshore areas over that to areas farther inland.
Sources and depositional processes of tsunami deposits: Analysis using foraminiferal tests and hydrodynamic verification
Authors: Uchida Jun‐Ichi , Fujiwara Osamu , Hasegawa Shiro , Kamataki Takanobu 
Published: August 2010

AbstractForaminiferal tests are commonly found in tsunami deposits and provide evidence of transport of sea floor sediments, sometimes from source areas more than 100 m deep and several kilometers away. These data contribute to estimates of the physical properties of tsunami waves, such as their amplitude and period. The tractive force of tsunami waves is inversely proportional to the water depth at sediment source areas, whereas the horizontal sediment transport distance by tsunami waves is proportional to the wave period and amplitude. We derived formulas for the amplitudes and periods of tsunami waves as functions of water depth at the sediment source area and sediment transport distance based on foraminiferal assemblages in tsunami deposits. We applied these formulas to derive wave amplitudes and periods from data on tsunami deposits in previous studies. For some examples, estimated wave parameters were reasonable matches for the actual tsunamis, although other cases had improbably large values. Such inconsistencies probably reflect: (i) local amplification of tsunami waves by submarine topography, such as submarine canyons; and (ii) errors in estimated water depth at the sediment source area and sediment transport distance, which mainly derive from insufficient identification of foraminiferal tests.
Health‐related quality of life and restless legs syndrome among women in Sweden
Authors: Wesström Jan , Nilsson Staffan , Sundström‐Poromaa Inger , Ulfberg Jan 
Published: August 2010

Aim:  Restless legs syndrome (RLS) is a common neurological movement disorder with a female preponderance, an increasing prevalence with age and comorbidity. Previous studies on the relationship between health‐related quality of life (HRQOL) and RLS are still sparse but knowledge is increasing. The aim of this study was to evaluate the unique impact of RLS on HRQOL in a population‐based sample.Methods:  A random sample of 5000 women aged 25–64 years was selected from the general Swedish population. The women were sent diagnostic questions on RLS together with the Short Form 12 (SF‐12) questionnaire for assessment of physical and mental HRQOL. The unique burden of RLS on HRQOL was analyzed by excluding RLS‐positive women from four self‐reported diagnostic groups (diabetes, depression, heart problems, muscle and joint pain) and by excluding subjects with these diagnoses from the group of RLS‐positive women.Results:  Compared with mental SF‐12 scores for the RLS‐negative women in our population, mental HRQOL of the RLS sample in our study was lower in every age group but not significantly lower in the age group 35–44 years. Physical SF‐12 scores for RLS‐positive women were also below scores for RLS‐negative women in every age group but significance was only found in women between 45 and 54 years. A unique burden of RLS on HRQOL remained after statistical adjustment for comorbidities.Conclusion:  RLS‐positive women had an impaired mental HRQOL compared to RLS‐negative women in the studied population. The physical aspects of HRQOL were less affected among RLS‐positive women. The impaired well‐being among women with RLS further strengthens the importance of identifying women with this condition and evaluating their need for medication or other actions in order to improve their quality of life.
Neurocognitive functioning in women presenting with undifferentiated somatoform disorders in Oman
Authors: Al‐Adawi Samir , Al‐Zakwani Ibrahim , Obeid Yousif A. , Zaidan Ziad 
Published: August 2010

Aim:  There is a dearth of research from non‐Western populations focusing on neurocognitive functioning in patients presenting with undifferentiated somatoform disorders. The aim of the present quest is to examine the presence of cognitive impairment and other health‐related parameters among attendees at psychiatric settings in Oman, an Arab/Islamic country, with a diagnosis of undifferentiated somatoform disorder.Method:  In order to compare the performance of patients diagnosed with undifferentiated somatoform disorder (n = 20) and normal healthy subjects (n = 18) on indices of attention and concentration, tests of executive functioning, mood, somatization and vegetative functioning were carried out.Results:  The performance of patients with undifferentiated somatoform disorder differed from that of normal healthy subjects on the presently operationalized indices of working memory and executive functioning, anxiety, quality of sleep and psychosomatically expressed psychological distress.Conclusion:  To our knowledge, this is the first study highlighting neurocognitive functioning in patients with undifferentiated somatoform disorders from a non‐Western population. This type of study has the potential of shedding light on the covariates of such a debilitating and intransigent condition as undifferentiated somatoform disorder.
ECTOPARASITES OF THE EUROPEAN HARE (LEPUS EUROPAEUS (PALLAS)) IN TWO REGIONS OF VICTORIA
Authors: SHEPHERD ROSAMOND C. H. , NOLAN I. F. , LANE I. L. , EDMONDS J. W. 
Published: August 2010

AbstractHares from the Mallee and central regions of Victoria were examined for ectoparasites. Hares from the Mallee carried the stickfast flea Echidnophaga myrmecobii and the mite Leporacarus gibbus. Hares from central Victoria carried the lice Haemodipsus setoni and H. lyriocephalus. H. setoni was found mainly on the body of the hare, H. lyriocephalus on the head.The recently introduced rabbit flea, Spilopsyllus cuniculi was found on hares in both regions.
THE ROLE OF DISEASE DURING AN OUTBREAK OF ONCOPERA ALBOGUTTATA TINDALE AND O. RUFOBRUNNEA TINDALE. (LEPIDOPTERA: HEPIALIDAE) IN THE EBOR/DORRIGO REGION OF N.S.W.
Authors: MILNER R. J. 
Published: August 2010

AbstractTwo diseases, caused by a microsporidan Pleistophora oncoperae Milner and Beaton and pox virus, were found to be common and widespread in populations of Oncopera alboguttata. In contrast, disease was rare in O. rufobrunnea populations and only P. oncoperae was found. Studies of field populations of O. alboguttata between 1971 and 1974 indicated that disease was responsible for the dramatic decrease in population density which occurred during 1973.
THE INSECT PATHOGENIC FUNGUS CULICINOMYCES IN ADULTS OF THE MOSQUITO ANOPHELES AMICTUS HILLI
Authors: DEBENHAM M. L. , RUSSELL R. C. 
Published: August 2010

AbstractThe insect pathogenic fungus Culicinomyces has been recorded infecting larval mosquitoes in Sydney and in North Carolina, U.S.A. The first discovery of the fungus in adult mosquitoes is here recorded, with evidence that infected adults are themselves infective to larvae. It is suggested that this interstadial infection could contribute to the natural dispersal of the fungus.
THE GENUS MILLOTELLINA JUPEAU IN AUSTRALIA (MYRIAPODA: SYMPHYLA: SCUTIGERELLIDAE)
Authors: NAUMANN I. D. , SCHELLER ULF 
Published: August 2010

AbstractThe genus Millotellina is reported from Australia for the first time and divided into two subgenera: Millotellina sens. str. (with unpaired sternal appendages) and Diplomillotellina Scheller subgen. n. (with paired sternal appendages). M. (M.) monteithi Naumann sp. n.is described from northern Australia and M. (D.) bidens Scheller sp. n. from southern mainland Australia.
THE OCCURRENCE IN AUSTRALIA OF LACHNOPHOROIDES DISTANT (HETEROPTERA: LYGAEIDAE) WITH DESCRIPTIONS OF TWO NEW SPECIES
Authors: WOODWARD T. E. 
Published: August 2010

AbstractThe distribution of the rhyparochromine genus Lachnophoroides Distant, 1914 (Targaremini), previously known only by two species from New Caledonia, is extended to Queensland with the description of L. thompsoni sp. n. A third New Caledonian species, L. monteithorum sp. n., is described. A specific key is provided and the immature stages of L. thompsoni are described and the nymphal instars keyed out.