bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect isolated lactic acid bacteria inoculation on the quality, bacterial composition and metabolic characterization of Caragana korshinskii silage
Authors: Bai Baochao , Qiu Rui , Sun Lin , Bao Jian , Liu Yichao , Ge Gentu , Jia Yushan , Wang Zhijun 
Published: May 2024

AbstractFeed shortage has hindered the development of the world’s livestock industry, and the rational use of non-conventional forages can help resolve this issue. To improve the fermentation quality of Caragana korshinskii silage, this study isolated lactic acid bacteria (LAB) from fresh Caragana korshinskii and silage samples, as alternatives to traditional commercial LAB inoculants. Utilizing Single Molecule, Real-Time (SMRT) sequencing technology and metabolomics analysis, the microbial community structure and metabolome were examined after 60 days of fermentation to elucidate the mechanism by which isolated LAB affect the fermentation quality of Caragana korshinskii silage. After 60 days of ensiling, the dominant microbial population in the silage shifted from harmful bacteria such as Erwinia, Pantoea, and Enterobacter to LAB. Compared to commercial LAB, the isolated Lactiplantibacillus plantarum significantly reduced the microbial diversity of Caragana korshinskii silage. The species composition of LAB became simpler with Lentilactobacillus parakefiri dominating and achieving a higher relative abundance. By down-regulating Butanoate metabolism and the Pentose phosphate pathway and up-regulating the Ascorbate and aldarate metabolism pathway, the isolated Lactiplantibacillus plantarum significantly lowered the pH of the silage, increased the content of water-soluble carbohydrates (WSC) and lactic acid (LA), reduced the neutral detergent fiber content (NDF), and improved in vitro neutral detergent fiber digestibility (IVNDFD), thereby enhancing the fermentation quality of Caragana korshinskii silage. On the other hand, the isolated Limosilactobacillus fermentum had the opposite effect, with its ensiling outcome being less effective than that of commercial LAB. The results of the study indicate that the addition of the isolated Lactiplantibacillus plantarum improved the quality of Caragana korshinskii silage by regulating metabolic pathways and the composition of microbes within the ensiled feed.
Graphical Abstract
Can linezolid be validly measured in endotracheal aspiration in critically ill patients? A proof-of-concept trial
Authors: Rebholz Diana , Liebchen Uwe , Paal Michael , Vogeser Michael , Starp Johannes , Gräfe Caroline , Brozat Clara I. , Happich Felix L. , Habler Katharina , Scharf Christina 
Published: May 2024

Abstract
Background
Therapeutic drug monitoring (TDM) of anti-infectives such as linezolid is routinely performed in blood of intensive care unit (ICU) patients to optimize target attainment. However, the concentration at the site of infection is considered more important for a successful therapy. Until now, bronchoalveolar lavage (BAL) is the gold standard to measure intrapulmonary concentrations of anti-infective agents. However, it is an invasive method and unsuitable for regular TDM. The aim of this proof-of-concept study was to investigate whether it is possible to reliably determine the intrapulmonary concentration of linezolid from endotracheal aspiration (ENTA).

Methods
Intubated ICU patients receiving 600 mg intravenous linezolid twice daily were examined in steady state. First, preliminary experiments were performed in six patients to investigate which patients are suitable for linezolid measurement in ENTA. In a second step, trough and peak linezolid concentrations of plasma and ENTA were determined in nine suitable patients.

Results
Linezolid can validly be detected in ENTA with viscous texture and > 0.5 mL volume. The mean (SD) linezolid trough concentration was 2.02 (1.27) mg/L in plasma and 1.60 (1.36) mg/L in ENTA, resulting in a median lung penetration rate of 104%. The mean (SD) peak concentration in plasma and ENTA was 10.77 (5.93) and 4.74 (2.66) mg/L.

Conclusions
Linezolid can validly be determined in ENTA with an adequate texture and volume. The penetration rate is comparable to already published BAL concentrations. This method might offer a simple and non-invasive method for TDM at the site of infection “lung”. Due to promising results of the feasibility study, comparison of ENTA and BAL in the same patient should be investigated in a further trial.
Integrating depth-dependent protist dynamics and microbial interactions in spring succession of a freshwater reservoir
Authors: Mukherjee Indranil , Grujčić Vesna , Salcher Michaela M. , Znachor Petr , Seďa Jaromír , Devetter Miloslav , Rychtecký Pavel , Šimek Karel , Shabarova Tanja 
Published: May 2024

Abstract
Background
Protists are essential contributors to eukaryotic diversity and exert profound influence on carbon fluxes and energy transfer in freshwaters. Despite their significance, there is a notable gap in research on protistan dynamics, particularly in the deeper strata of temperate lakes. This study aimed to address this gap by integrating protists into the well-described spring dynamics of Římov reservoir, Czech Republic. Over a 2-month period covering transition from mixing to established stratification, we collected water samples from three reservoir depths (0.5, 10 and 30 m) with a frequency of up to three times per week. Microbial eukaryotic and prokaryotic communities were analysed using SSU rRNA gene amplicon sequencing and dominant protistan groups were enumerated by Catalysed Reporter Deposition-Fluorescence in situ Hybridization (CARD-FISH). Additionally, we collected samples for water chemistry, phyto- and zooplankton composition analyses.

Results
Following the rapid changes in environmental and biotic parameters during spring, protistan and bacterial communities displayed swift transitions from a homogeneous community to distinct strata-specific communities. A prevalence of auto- and mixotrophic protists dominated by cryptophytes was associated with spring algal bloom-specialized bacteria in the epilimnion. In contrast, the meta- and hypolimnion showcased a development of a protist community dominated by putative parasitic Perkinsozoa, detritus or particle-associated ciliates, cercozoans, telonemids and excavate protists (Kinetoplastida), co-occurring with bacteria associated with lake snow.

Conclusions
Our high-resolution sampling matching the typical doubling time of microbes along with the combined microscopic and molecular approach and inclusion of all main components of the microbial food web allowed us to unveil depth-specific populations’ successions and interactions in a deep lentic ecosystem.
Bibliometric study on organizational resilience: trends and future research agenda
Authors: Mhlanga David , Dzingirai Mufaro 
Published: May 2024

AbstractIn an increasingly volatile, uncertain, complex, and ambiguous (VUCA) world, the importance of organizational resilience has grown, yet the scholarly literature on this topic remains fragmented. To address this gap, our study conducted a bibliometric analysis of 469 articles from the Scopus database using VOSViewer software to systematically review and map trends, gaps, and significant contributions in the field. Our analysis revealed key themes such as resilience, crisis management, innovation, COVID-19, dynamic capabilities, sustainability, and change management, which are crucial to understanding organizational resilience. The findings highlight that the United Kingdom and the University of Oulu are significant contributors to this research area, with notable authors including Duchek E., Martinelli E., Santoro G., Williams T.A., and Youssef C.M. playing a pivotal role in advancing this field. By providing a comprehensive overview of institutional affiliations, countries, authors, journals, publications, and keyword co-occurrences, our study not only maps the landscape of organizational resilience research but also identifies critical areas for future inquiry. This contribution enhances both theoretical and practical understandings of organizational resilience, aiding practitioners in developing robust strategies to navigate the challenges of the VUCA world.
Translational simulation revisited: an evolving conceptual model for the contribution of simulation to healthcare quality and safety
Authors: Brazil Victoria , Reedy Gabriel 
Published: May 2024

AbstractThe simulation community has effectively responded to calls for a more direct contribution by simulation to healthcare quality and safety, and clearer alignment with health service priorities, but the conceptual framing of this contribution has been vague. The term ‘translational simulation’ was proposed in 2017 as a “functional term for how simulation may be connected directly with health service priorities and patient outcomes, through interventional and diagnostic functions” (Brazil V. Adv Simul. 2:20, 2017). Six years later, this conceptual framing is clearer. Translational simulation has been applied in diverse contexts, affording insights into its strengths and limitations. Three core concepts are identifiable in recently published translational simulation studies: a clear identification of simulation purpose, an articulation of the simulation process, and an engagement with the conceptual foundations of translational simulation practice. In this article, we reflect on current translational simulation practice and scholarship, especially with respect to these three core concepts, and offer a further elaborated conceptual model based on its use to date.
Comparative study of the reptilian cornea's microstructure
Authors: Abdelftah Zeinab , Ragab Ahmed , Abo-Eleneen Rasha E. , Abuelsaad Abdelaziz S. , EL-Bakry Ahlam M. 
Published: May 2024

Abstract
Background
The cornea of various vertebrate is considered a major part in the glop, which acts as a powerful lens, providing a sharp retinal image, and meantime acts as an excellent defensor for other corneal layers.

Results
The four reptilian families [Scincidae (Chalcides ocellatus); Chamaeleontidae (Chameleon chameleon); Cheloniidae (Chelonia mydas) and Testudiniae (Testudo kleinmanni)] investigated in the current study were gathered across Egypt. After being taken out of the orbit, the cornea under inquiry was ready for light, specific stain and scanning electron microscopy. The epithelium, stroma, and endothelium are the three corneal layers that are common to all four species of reptiles. All other species lack Bowmen's and Descemet's membranes, with the exception of C. ocellatus and T. kleinmanni. The latter layers of Chalcides ocellatus display a strong affinity for Periodic Acid Schiff stain. Epithelial cells with a variety of forms, from hexagonal to atypical polygonal cells, cover the outer corneal surface. These epithelial cells are coated in short microplicae, microvilli, and microholes of varied diameters. There are a few blebs scattered around their surface.

Conclusion
The current study concluded that various habitats have various significant effects on the cornea's microstructure characteristics and its physiological trends to accommodate different environmental surroundings.
Association of auditory processing abilities and employment in young women
Authors: Sharma Yoshita , K.V. Nisha 
Published: May 2024

Abstract
Background
This study aimed to investigate the relationship between employment status and auditory processing abilities in women, considering the cognitive impact of work-related demands. The objective was to study the influence of employment on forward -, backward -, operation span, 2n back, and speech perception in noise (SPIN) scores.

Methods
Fifty-eight women aged 20 to 64 years were categorized into working and non-working groups. Auditory cognitive assessments included forward and backward digit span, auditory 2n-back, operational span, and speech perception in noise. Mental workload, listening effort, and fatigue levels were evaluated for each test using standardized scales. Handedness was assessed using the Edinburgh Handedness Inventory. Statistical analysis involved Mann–Whitney tests and effect size calculations.

Results
Working women scored significantly higher in 2n-back task compared to non-working counterparts, emphasizing the positive impact of employment on working memory and cognitive control processes. The working women group perceptually rated greater mental load on the operational span task than non-working group, indicating the task-specific manifestation of group differences. When the tasks are easier (2n back) or difficult (operation span), employed women exhibited an advantage in auditory processing over their counterparts.

Conclusion
This study reveals nuanced cognitive differences influenced by employment status in women. Working women demonstrated superior auditory processing abilities, specifically in working memory tasks, suggesting potential cognitive benefits associated with engagement in the workforce. The elevated mental workload for dual task working memory paradigms underscores the complexity of simultaneous processing and storage, highlighting cognitive challenges in specific occupational contexts.
A systematic review of telemedicine for neuromuscular diseases: components and determinants of practice
Authors: Senyel Deniz , Senn Katja , Boyd James , Nagels Klaus 
Published: May 2024

Abstract
Introduction
Neuromuscular diseases (NMDs) entail a group of mostly inherited genetic disorders with heterogeneous phenotypes impacting muscles, the central or peripheral nervous system. They can lead to severe disabilities and shortened lifespans. Despite their severity, NMDs often lack in public awareness and appropriate medical and social support. Telemedicine can improve patients’ and caregivers’ lives by enhancing continuity of and access to care. The first aim of this systematic review was to summarise the status quo of telemedicine services for patients with NMDs. Secondly, barriers and facilitators of the respective implementation processes should be analysed.

Methods
The databases PubMed, Web of Science and CENTRAL by Cochrane were searched in May 2022. To be truly explorative, any original evidence from any setting was included. Two independent researchers completed the screening process. Data was extracted and analysed using the taxonomy of Bashshur et al. (2011) and the Consolidated Framework for Implementation Research (CFIR).

Results
Fifty-seven original papers were included in the systematic review. The results showed a high representation of teleconsultations and remote monitoring studies. Teleconsultations replaced in person appointments and telemonitoring mostly focused on ventilation. Physical therapy, pulmonology, neurology, and psychology were the most represented medical specialties. We found barriers and facilitators relating to implementation mainly referred to the intervention and the individuals involved. Technical errors and inaccessibility due to a lack of technical devices or the patient’s disability were stated as hindrances. A positive mindset of users as well as patient empowerment were necessary for the adoption of new technology. Technophobia or uncertainty around technology negatively impacted the implementation process.

Discussion
This systematic review provides an overview of the current use of telemedicine in patients with NMDs. The distribution of telemedicine interventions between the defined domains was very heterogenous. Previous research has neglected to fully describe the implementation process of telemedicine for NMDs.

Conclusion
The evidence shows that telemedicine can benefit patients with NMDs in a multitude of ways. Therefore, health policies should endorse and incentivise the uptake of telemedicine by institutions and health care workers. Further research needs to be conducted to confirm the current evidence and close existing research gaps.
Moderate Associations Between the Use of Levonorgestrel-releasing Intrauterine Device and Metabolomics Profile
Authors: Toffol Elena , Heikinheimo Oskari , Jousilahti Pekka , Lehtoranta Lara , Joensuu Anni , Partonen Timo , Erlund Iris , Haukka Jari 
Published: May 2024

Abstract

Context
Use of a levonorgestrel-releasing intrauterine device (LNG-IUD) has become common irrespective of age and parity. To date, only a few studies have examined its possible metabolic changes and large-scale biomarker profiles in detail and in a longitudinal design.

Objective
To apply the metabolomics technique to examine the metabolic profile associated with the use of LNG-IUD both in a cross-sectional and a longitudinal design.

Design
The study consists of cross-sectional and longitudinal analyses of a population-based survey (Health 2000) and its 11-year follow-up (Health 2011). All participants aged 18 to 49 years with available information on hormonal contraceptive use and metabolomics data (n = 1767) were included. Altogether 212 metabolic measures in LNG-IUD users (n = 341) were compared to those in non-users of hormonal contraception (n = 1426) via multivariable linear regression models. Participants with complete longitudinal information (n = 240) were divided into continuers, stoppers, starters, and never-user groups, and 11-year changes in levels of each metabolite were compared.

Results
After adjustment for covariates, levels of 102 metabolites differed in LNG-IUD current users compared to non-users of hormonal contraception (median difference in biomarker concentration: –0.12 SD): lower levels of fatty acids concentrations and ratios, cholesterol, triglycerides and other lipids, as well as particle concentration, cholesterol, total lipids, and phospholipids in lipoproteins. The 11-year metabolic changes did not differ in relation to changes in LNG-IUD use.

Conclusion
The use of LNG-IUD was associated with several moderate metabolic changes, mostly suggestive of a reduced arterial cardiometabolic risk. Changes in LNG-IUD use were not related to long-term metabolic changes.
Interrogating Estrogen Signaling Pathways in Human ER-Positive Breast Cancer Cells Forming Bone Metastases in Mice
Authors: Cheng Julia N , Frye Jennifer B , Whitman Susan A , Ehsani Sima , Ali Simak , Funk Janet L 
Published: May 2024

Abstract
Breast cancer bone metastases (BMET) are incurable, primarily osteolytic, and occur most commonly in estrogen receptor-α positive (ER+) breast cancer. ER+ human breast cancer BMET modeling in mice has demonstrated an estrogen (E2)-dependent increase in tumor-associated osteolysis and bone-resorbing osteoclasts, independent of estrogenic effects on tumor proliferation or bone turnover, suggesting a possible mechanistic link between tumoral ERα-driven osteolysis and ER+ bone progression. To explore this question, inducible secretion of the osteolytic factor, parathyroid hormone–related protein (PTHrP), was utilized as an in vitro screening bioassay to query the osteolytic potential of estrogen receptor- and signaling pathway–specific ligands in BMET-forming ER+ human breast cancer cells expressing ERα, ERß, and G protein–coupled ER. After identifying genomic ERα signaling, also responsibility for estrogen's proliferative effects, as necessary and sufficient for osteolytic PTHrP secretion, in vivo effects of a genomic-only ER agonist, estetrol (E4), on osteolytic ER+ BMET progression were examined. Surprisingly, while pharmacologic effects of E4 on estrogen-dependent tissues, including bone, were evident, E4 did not support osteolytic BMET progression (vs robust E2 effects), suggesting an important role for nongenomic ER signaling in ER+ metastatic progression at this site. Because bone effects of E4 did not completely recapitulate those of E2, the relative importance of nongenomic ER signaling in tumor vs bone cannot be ascertained here. Nonetheless, these intriguing findings suggest that targeted manipulation of estrogen signaling to mitigate ER+ metastatic progression in bone may require a nuanced approach, considering genomic and nongenomic effects of ER signaling on both sides of the tumor/bone interface.
Frontiers in Operations: Valuing Nursing Productivity in Emergency Departments
Authors: Ding Hao , Tushe Sokol , KC Diwas Singh , Lee Donald K. K. 
Published: May 2024

Problem definition: We quantify the increase in productivity in emergency departments (EDs) from increasing nurse staff. We then estimate the associated revenue gains for the hospital and the associated welfare gains for society. The United States is over a decade into the worst nursing shortage crisis in history fueled by chronic underinvestment. To demonstrate to hospital managers and policymakers the benefits of investing in nursing, we clarify the positive downstream effects of doing so in the ED setting. Methodology/results: We use a high-resolution data set of patient visits to the ED of a major U.S. academic hospital. Time-dependent hazard estimation methods (nonparametric and parametric) are used to study how the real-time service speed of a patient varies with the state of the ED, including the time-varying workloads of the assigned nurse. A counterfactual simulation is used to estimate the gains from increasing nursing staff in the ED. We find that lightening a nurse’s workload by one patient is associated with a 14% service speedup for every patient under the nurse’s care. Simulation studies suggest that adding one more nurse to the busiest 12-hour shift of each day can shorten stays and avert $160,000 in lost patient wages per 10,000 visits. The reduction in service times also frees up capacity for treating more patients and generates $470,000 in additional net revenues for the hospital per 10,000 visits. Extensive sensitivity analyses suggest that our key message—that investing in nursing will more than pay for itself—is likely to hold across a wide range of EDs. Managerial implications: In determining whether to invest in more nursing resources, hospital managers need to look beyond whether payer reimbursements alone are sufficient to cover the up-front costs to also account for the resulting downstream benefits. History: This paper has been accepted in the Manufacturing & Service Operations Management Frontiers in Operations Initiative. Funding: D. K. K. Lee was supported by the National Heart, Lung, and Blood Institute [Grant R01-HL164405]. Supplemental Material: The online appendix is available at https://doi.org/10.1287/msom.2023.0039 .
CLASSIFICATION OF FREIBERG'S DISEASE: A GUIDE FOR MANAGEMENT
Authors: Nanavati N. , Davies M. , Blundell C. , Flowers M. , Chadwick C. , Davies H. 
Published: May 2024

IntroductionThe current treatment for Freiberg's osteochondrosis centres around either: simple debridement or debridement osteotomy. The main principle of the osteotomy is to rotate normal articular cartilage into the affected area. We recommend the use of CT scanning to delineate the amount of available, unaffected cartilage available to rotate into the affected space.MethodsWe retrospectively reviewed 32 CT scans of new Freiberg's diagnoses in Sheffield over a 10 year period using the PACS system. We identified the sagittal CT slice that displayed the widest portion of proximal articular margin of the proximal phalanx and measured the diseased segment of the corresponding metatarsal head as an arc (in degrees). This arc segment was divided by 360°. This gave a ratio of the affected arc in the sagittal plane.Results28 out of 32 cases involved the 2nd metatarsal with the remaining 4 involving the 3rd metatarsal head. Of 32 cases, 18 had fragmentation. Surgically, 20 had debridement only, 5 also had an osteotomy and 1 had a fusion. 6 of the 32 cases were managed non-operatively. 11 cases out of 32 had an arc ratio of < 0.3. Of these, only 3 had an osteotomy, 3 had no procedure and 5 had a simple debridement. Of those that had osteotomies (5/32), 3 of the 5 cases had an arc ratio of < 0.3 with the other 2 being 0.42 and 0.38.DiscussionWe hypothesise that those cases with an arc ratio of less 0.3 would be amenable to a dorsal closing wedge osteotomy and those with a ratio of more than 0.4 would be better suited to a simple debridement. For those cases between 0.3–0.4, we feel either option is viable. Further work to prove or disprove outcomes related to our classification is required.