bluuebunny/crossref_metadata_2025_split huggingface dataset:
Osteoblast response to puerarin‐loaded porous titanium surfaces: An in vitro study
Authors: Yang Fan , Zhang Rong , He Fuming , Wang Xiao‐Xiang , Zhao Shifang , Yang Guoli 
Published: February 2012

AbstractRecent studies demonstrate puerarin stimulates bone formation, suggesting its potential application in dental implantology field. The aim of this study was to investigate effects of puerarin‐loaded titanium surfaces on the promotion of osteogenesis in preosteoblasts (MC3T3‐E1). Puerarin was prepared onto titanium surfaces with varying concentration (10−9M, 10−8M, 10−7M, and 10−6M) by biomimetic calcium phosphate deposition process. Surface characteristics were performed by field‐emission scanning electron microscope (FSEM), X‐ray diffractometer (XRD), and Fourier transform infrared spectroscopy (FTIR). Puerarin concentration in the coatings was performed by High‐performance liquid chromatograph (HPLC) analyses. FSEM observation showed puerarin concentration influenced formation of sharp flakes in the coating. The size of flakes decreased with increase of puerarin concentrations. XRD and FTIR examinations demonstrated the puerarin concentration did not affect the chemical compositions of coatings, which composed of octacalcium phosphate (OCP). Puerarin concentration on the surfaces of 10−8M group was 10.22 ± 0.32 ng/cm2. Puerarin had an increased effect on MC3T3‐E1 ALP activities. Significant differences were found in 10−8 and 10−7M groups on day 4, 10−8, 10−7, and 10−6M groups on day 7, and 10−8 on day 14. In Type I collagen synthesis assay, 10−9 and 10−8M on day 7, 10−8 on day 14 showed significant differences compared with control group. Furthermore, this stimulatory effect of puerarin was also observed in osteocalcin release assay (p < 0.05, at 10−8M and 10−7M, maximal at 10−8M). These results indicate puerarin‐loaded titanium surfaces promote accelerated osteogenic differentiation of preosteoblasts, which has the potential to improve the nature of osseointegration. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A:, 2012.
Enabling grand‐canonical Monte Carlo: Extending the flexibility of GROMACS through the GromPy python interface module
Authors: Pool René , Heringa Jaap , Hoefling Martin , Schulz Roland , Smith Jeremy C. , Feenstra K. Anton 
Published: February 2012

AbstractWe report on a python interface to the GROMACS molecular simulation package, GromPy (available at https://github.com/GromPy). This application programming interface (API) uses the ctypes python module that allows function calls to shared libraries, for example, written in C. To the best of our knowledge, this is the first reported interface to the GROMACS library that uses direct library calls. GromPy can be used for extending the current GROMACS simulation and analysis modes. In this work, we demonstrate that the interface enables hybrid Monte‐Carlo/molecular dynamics (MD) simulations in the grand‐canonical ensemble, a simulation mode that is currently not implemented in GROMACS. For this application, the interplay between GromPy and GROMACS requires only minor modifications of the GROMACS source code, not affecting the operation, efficiency, and performance of the GROMACS applications. We validate the grand‐canonical application against MD in the canonical ensemble by comparison of equations of state. The results of the grand‐canonical simulations are in complete agreement with MD in the canonical ensemble. The python overhead of the grand‐canonical scheme is only minimal. © 2012 Wiley Periodicals, Inc.
Helicobacter pylori genotype and polymorphisms in DNA repair enzymes: Where do they correlate in gastric cancer?
Authors: Silva‐Fernandes Isabelle Joyce de Lima , da Silva Thayse Azevedo , Agnez‐Lima Lucymara Fassarella , Ferreira Márcia Valéria Pitombeira , Rabenhorst Silvia Helena Barem 
Published: February 2012

AbstractBackground and ObjectivesOne of the mechanisms proposed by which H. pylori causes gastric cancer (GC) is through DNA damage due to chronic inflammation. Genomic integrity is guaranteed by repair enzymes such as APE‐1, OGG‐1, and PARP‐1. Host genetic polymorphisms associated with the bacterial strain may influence the ability to repair the damage, contributing to the development of H. pylori‐associated GC. The aim of this study was to determine the association of the polymorphisms APE‐1 (T2197G), OGG‐1 (C1245G), and PARP‐1 (A40676G) with H. pylori‐genotype in 109 patients with GC.MethodsPolymorphism was assessed by polymerase chain reaction (PCR)‐restriction fragment length polymorphism (RFLP) and H. pylori detection/genotyping by PCR.ResultsIn the intestinal subtype, PARP‐1 wild‐type was more frequent (P = 0.001) in patients >50 years old. The repair enzymes genotypes analyzed in combination showed that the less pathogenic strains are associated with the APE‐1 polymorphic allele and, unexpectedly, with PARP‐1 wild‐type, but this last one associated with APE‐1 polymorphic allele or in older patients.ConclusionsOur results indicate the importance of H. pylori and APE‐1 genotypes in the gastric carcinogenesis. Also, support the hypothesis of a decrease of PARP‐1 wild‐type activity in older individuals. Taken together these data may be an important clue to understand the role of low‐virulence strains of H. pylori in gastric carcinogenesis and point the importance to analyze the polymorphisms as a group. J. Surg. Oncol. 2012; 106:448–455. © 2012 Wiley Periodicals, Inc.
Policy learning and deer co‐management in New York State: Insights from newspaper coverage
Authors: Triezenberg Heather A. , Lauber T. Bruce , Knuth Barbara A. 
Published: February 2012

AbstractResolution of urban and suburban deer problems often depends on learning and co‐management. Local newspapers may reflect the learning occurring in a community. Past authors have identified 4 primary types of learning (technical, conceptual, social, and political). We studied newspaper content related to deer management for insights about the types of learning occurring in selected regions in New York State. We analyzed 263 newspaper articles published between 2000 and 2006. We found that the majority of content related to learning focused on conceptual and technical learning, but relatively little, particularly early in the evolution of deer management issues, focused on social or political learning. Our results suggest that additional attention to social learning focusing on collaborative relationships and constructive dialogue among stakeholders by both communities and newspapers could be beneficial in the resolution of deer problems. © 2012 The Wildlife Society.
Using Bayesian networks to incorporate uncertainty in habitat suitability index models
Authors: Wilhere George F. 
Published: February 2012

AbstractHabitat suitability index (HSI) models rarely characterize the uncertainty associated with their estimates of habitat quality despite the fact that uncertainty can have important management implications. The purpose of this paper was to explore the use of Bayesian belief networks (BBNs) for representing and propagating 3 types of uncertainty in HSI models—uncertainty in the suitability index relationships, the parameters of the HSI equation, and measurement of habitat variables (i.e., model inputs). I constructed a BBN–HSI model, based on an existing HSI model, using Netica™ software. I parameterized the BBN's conditional probability tables via Monte Carlo methods, and developed a discretization scheme that met specifications for numerical error. I applied the model to both real and dummy sites in order to demonstrate the utility of the BBN–HSI model for 1) determining whether sites with different habitat types had statistically significant differences in HSI, and 2) making decisions based on rules that reflect different attitudes toward risk—maximum expected value, maximin, and maximax. I also examined effects of uncertainty in the habitat variables on the model's output. Some sites with different habitat types had different values forE[HSI], the expected value of HSI, but habitat suitability was not significantly different based on the overlap of 90% confidence intervals forE[HSI]. The different decision rules resulted in different rankings of sites, and hence, different decisions based on risk. As measurement uncertainty in habitat variables increased, sites with significantly different (α = 0.1)E[HSI] became statistically more similar. Incorporating uncertainty in HSI models enables explicit consideration of risk and more robust habitat management decisions. © 2012 The Wildlife Society.
Electrochemical oxidation of 4‐tert‐butylcatechol in the presence of β‐cyclodextrin: Interplay between E and CE mechanisms
Authors: Rafiee Mohammad 
Published: February 2012

AbstractElectrochemical behavior of 4‐tert‐butylcatechol (H2Q) in the presence of β‐cyclodextrin (β‐CD) was studied using cyclic voltammetry and hydrodynamic voltammetry. An electrochemical model in which both H2Q and its oxidized form (Q) created inclusion complexes with β‐CD was proposed, and it was concluded that both free (as a result of complex‐dissociation reaction) and complex species could performed electron transfer. The heterogeneous rate constant for electron transfer of the inclusion complexes and their kinetic and thermodynamic parameters were obtained using digital simulation. © 2012 Wiley Periodicals, Inc. Int J Chem Kinet 44: 507–513, 2012
Topical glucocorticoid reduces the topical decongestant–induced histologic changes in an animal model nasal mucosa
Authors: Akpinar Meltem Esen , Yigit Ozgur , Akakin Dilek , Sarioz Ozlem , Ozkan Naziye , Yildiz Sercan D. , Azizli Elad , Sehirli Umit S. 
Published: February 2012

AbstractObjectives/Hypothesis:To investigate the histologic consequences of simultaneous nasal glucocorticosteroid and xylometazoline HCl administration in the rabbit nasal mucosa.Study Design:Prospective randomized study.Methods:Twenty New Zealand male rabbits were randomly placed into three groups: group I, control (n = 6); group II, xylometazoline HCl (n = 8); or group III, xylometazoline HCl–fluticasone furoate (n = 6). Group I received no treatment. Groups II and III received two intranasal puffs of xylometazoline HCl 0.5 mg/mL twice daily or two puffs of xylometazoline HCl 0.5 mg/mL twice daily plus one puff of 27.5 μg fluticasone furoate twice daily to each nostril (110 μg), respectively. At the end of 3 weeks, the rabbits were sacrificed. The mucosa of the nasal cavities was excised. Specimen sections (5 μm) were stained with hematoxylin and eosin, mucicarmine, and Gomori one‐step trichrome and were examined under a light microscope. The presence of edema, congestion, inflammatory cell infiltration, nasociliary loss, epithelial and nerve‐ending degeneration, and goblet cell increase were evaluated semiquantitatively (grades 0–3).Results:Statistically significant differences were detected between groups II and III in terms of edema, congestion, inflammatory cell infiltration, nasociliary loss, and epithelial degeneration (P = .006, P = .049, P = .015, P = .014, and P = .049, respectively). Nerve‐ending degeneration, goblet cell increase, and quantitative goblet and neutrophil cell counts did not yield statistically significant differences between groups II and III (P = .137, P = .580, P = .770, and P = .616, respectively).Conclusions:The combined simultaneous intranasal administration of xylometazoline HCl and fluticasone furoate appears to be beneficial in minimizing the long‐term usage–associated congestion, edema, inflammatory cell infiltration, epithelial degeneration, and nasociliary loss in the rabbit model nasal mucosa. Laryngoscope, 2012
Early changes in apparent diffusion coefficients predict radiosensitivity of human nasopharyngeal carcinoma xenografts
Authors: Pan Jianji , Zang Lele , Zhang Yu , Hong Jinsheng , Yao Yiqi , Zou Changyan , Zhang Lurong , Chen Yunbin 
Published: February 2012

AbstractObjectives/Hypothesis:Our objective was to predict the radiosensitivity of human nasopharyngeal carcinoma xenografts in nude mice models through an examination of early changes in apparent diffusion coefficient (ADC) values.Study Design:Randomized.Methods:BALB/c‐nu nude mice (n = 20) were divided into two groups that were subcutaneously injected with CNE1 or CNE2 cell lines. Xenograft volumes were measured after tumor formation, mice were scanned with a diffusion‐weighted imaging sequence, and the mean ADC values were measured (ADC0). Fifteen to 20 hours after tumors received 15 Gy, mice were scanned again and ADC values (ADC1) were measured.Results:ADC0 and ADC1 values of the CNE1 group showed no significant difference (P = .692). The difference between the ADC0 and ADC1 values of the CNE2 group was statistically significant (P < .001). ADC0 values of the two groups exhibited no statistically significant difference (P = .204). ADC1, ADC1‐0, and ΔADC of the two groups exhibited statistically significant differences (P < .001; P = .001 and .002, respectively). After irradiation, volume changes ΔV8, ΔV10, and ΔV12 of two groups were statistically different (all P < .001). Pearson correlation analysis showed ADC1‐0 and ΔADC were positively correlated with ΔV8, ΔV10, and ΔV12. The cut point was found by means of a receiver operating characteristic curve, and the ΔV12 of the two redivided groups showed a statistically significant difference (P = .001).Conclusions:This study found that changes in ADC values correlated with volume changes after irradiation. Therefore, ADC values have the potential to predict the radiosensitivity of nasopharyngeal carcinoma xenografts. Laryngoscope, 2012
Variability in the perilymphatic diffusion of gadolinium does not predict the outcome of intratympanic gentamicin in patients with ménière's disease
Authors: Fiorino Francesco , Pizzini Francesca B. , Barbieri Franco , Beltramello Alberto 
Published: February 2012

AbstractObjectives/Hypothesis:To assess the utility of imaging in planning intratympanic (IT) gentamicin (Gent) treatment in Ménière's disease (MD), we compared the dosage and outcomes of ITGent with the severity and extent of endolymphatic hydrops (EH), as evaluated by three‐dimensional fluid‐attenuated inversion recovery (3D‐FLAIR) sequence in a 3‐T magnetic resonance imaging (MRI) unit, after IT gadolinium administration.Study Design:Retrospective review.Methods:A total of 18 patients (10 males and 8 females; age, 28–78 years; median age, 53.2 years) with definite MD participated in the investigation. The duration of the disease ranged from 8 months to 9 years (median, 2 years), with a prevalence of vertigo spells ranging from 0.8 to 8 per month (median, 2.2), as calculated in the last 6 months. A 3D‐FLAIR MRI was performed 24 hours after IT injection of diluted gadobutrol. ITGent injection was performed within a variable period of time, from 1 week to 3 weeks after 3D‐FLAIR MRI. The degree and extension of EH as evaluated by 3D‐FLAIR MRI were compared with the number of injections necessary to cure vertigo attacks. Vertigo results, functional level scale modifications, variations in caloric excitability, and pure‐tone average modifications.Results:No statistically significant correlation was observed between severity of EH and outcomes of ITGent administration.Conclusions:The hypothesis of a reduced effect of Gent administered intratympanically in the presence of severe EH, owing to obstacled diffusion along the perilymphatic compartments, has not been confirmed in the present investigation. Laryngoscope, 2012
The clinical application and efficacy of sodium hyaluronate–carboxymethylcellulose during tympanomastoid surgery
Authors: Ahn Joong Ho , Lim Hyun Woo , Hong Hye‐Ran 
Published: February 2012

AbstractObjectives/Hypothesis:To evaluate the anti‐adhesive and anti‐inflammatory effects of sodium hyaluronate–carboxymethylcellulose (HA‐CMC) on postoperative hearing improvement and complications during tympanomastoid surgery.Study Design:Prospective controlled clinical trial.Methods:We evaluated 287 patients who underwent type I tympanoplasty, with or without canal wall up mastoidectomy, between January 2007 and June 2010. Postoperative hearing and complications were compared in the 143 patients who received Gelfoam soaked with HA‐CMC during myringoplasty and the 144 patients who received Gelfoam only.Results:There were no significant between‐group differences in sex, age, and preoperative hearing status. However, average postoperative air‐bone gap (13.7 ± 8.5 dB vs. 17.2 ± 9.9 dB) and the number of air‐bone gaps smaller than 10 dB (40.6% vs. 24.3%) were significantly improved in the HA‐CMC compared with the control group. In addition, the rates of re‐otorrhea, reperforation of the tympanic membrane (TM), postoperative TM adhesion, and reoperation were lower in the HA‐CMC than in the control group without significances.Conclusions:These findings suggest that combined application of Gelfoam with HA‐CMC may be beneficial in patients undergoing tympanomastoid surgery. Laryngoscope, 2012
Effect of gender on the hearing performance of adult cochlear implant patients
Authors: Lenarz Minoo , Sönmez Hasibe , Joseph Gert , Büchner Andreas , Lenarz Thomas 
Published: February 2012

AbstractObjectives/Hypothesis:To evaluate the role of gender on the hearing performance of postlingually deafened adult patients with cochlear implants.Study Design:Individual retrospective cohort study.Methods:There were 638 postlingually deafened adults (280 men and 358 women) selected for a retrospective evaluation of their hearing performance with cochlear implants. Both genders underwent the same surgical and rehabilitative procedures and benefited from the latest technological advances available. There was no significant difference in the age, duration of deafness, and preoperative hearing performance between the genders. The test battery was composed of the Freiburger Monosyllabic Test, Speech Tracking, and the Hochmair‐Schulz‐Moser (HSM) sentence test in quiet and in 10‐dB noise. The results of 5 years of follow‐up are presented here.Results:Genders showed a similar performance in Freiburger Monosyllabic Test and Speech Tracking Test. However, in the HSM test in noise, men performed slightly better than women in all of the follow‐up sessions, which was statistically significant at 2 and 4 years after implantation.Conclusions:Although normal‐hearing women use more predictive cognitive strategies in speech comprehension and are supposed to have a more efficient declarative memory system, this may not necessarily lead to a better adaptation to the altered auditory information delivered by a cochlear implant. Our study showed that in more complex listening situations such as speech tests in noise, men tend to perform slightly better than women. Gender may have an influence on the hearing performance of postlingually deafened adults with cochlear implants.
Animal models of myoclonus: An overview
Authors: Nguyen Bang Q. , Kanthasamy Anumantha G. , Truong Daniel D. 
Published: February 2012

AbstractAttempts to characterize the mechanism(s) associated with myoclonus have led to the development of several naturally occurring and pharmacologically based animal models of myoclonus. Congenital disorders in animals that result in myoclonic seizures have been found in subpopulations of baboons that exhibit photoresponsive myoclonus and in herds of Hereford cattle that possess a fatal, autosomal‐inherited imbalance in spinal glycine neurotransmission. Pharmacologically based models of myoclonus use a variety of approaches to product myoclonic seizures in test animals.
Semi‐free radial forearm flap for head and neck reconstruction in vessel‐depleted neck after radiotherapy or radical neck dissection
Authors: Quilichini Julien , Benjoar Marc‐David , Hivelin Mikael , Brugel Lydia , Lantieri Laurent 
Published: February 2012

AbstractBackground:Venous thrombosis is the main cause of radial forearm flap failure, especially when recipient vessels are compromised by prior radiation therapy or neck dissection. In such conditions, semi‐free radial forearm flap (SF‐RFF) can be performed to reduce this risk.Patients and method:We reviewed all SF‐RFF procedures performed in our institution for head and neck reconstruction. The flap was harvested as a conventional radial forearm flap but the cephalic vein was dissected along the arm up to the deltopectoral crease and used as the sole drainage vein.Result:Seven SF‐RFFs were harvested for head and neck reconstructions. The dissection of the cephalic vein lasted less than 25 min in all cases. No flap loss or thrombosis was observed.Conclusion:The SF‐RFF is a reliable and versatile procedure for facial, oral, or larynx reconstruction. This hybrid version of the radial forearm free flap is particularly appropriate when no suitable recipient veins are available as a result of radiation or prior surgery. © 2012 Wiley Periodicals, Inc. Microsurgery, 2012.
Improved T2 mapping accuracy with dual‐echo turbo spin echo: Effect of phase encoding profile orders
Authors: Anderson Stephan W. , Sakai Osamu , Soto Jorge A. , Jara Hernan 
Published: February 2012

AbstractTurbo spin echo (TSE) pulse sequences have been applied to estimate T2 relaxation times in clinically feasible scan times. However, T2 estimations using TSE pulse sequences has been shown to differ considerable from reference standard sequences due to several sources of error. The purpose of this work was to apply voxel‐sensitivity formalism to correct for one such source of error introduced by differing phase encoding profile orders with dual‐echo TSE pulse sequences. The American College of Radiology phantom and the brains of two healthy volunteers were imaged using dual‐echo TSE as well as 32‐echo spin‐echo acquisitions and T2 estimations from uncorrected and voxel‐sensitivity formalism‐corrected dual‐echo TSE and 32‐echo acquisitions were compared. In all regions of the brain and the majority of the analyses of the American College of Radiology phantom, voxel‐sensitivity formalism correction resulted in considerable improvements in dual‐echo TSE T2 estimation compared with the 32‐echo acquisition, with improvements in T2 value accuracy ranging from 5.2% to 18.6%. Magn Reson Med, 2013. © 2012 Wiley Periodicals, Inc.
B1‐based specific energy absorption rate determination for nonquadrature radiofrequency excitation
Authors: Katscher Ulrich , Findeklee Christian , Voigt Tobias 
Published: February 2012

AbstractThe current gold standard to estimate local and global specific energy absorption rate for MRI involves numerically modeling the patient and the transmit radiofrequency coil. Recently, a patient‐individual method was presented, which estimated specific energy absorption rate from individually measured B1 maps. This method, however, was restricted to quadrature volume coils due to difficulties distinguishing phase contributions from radiofrequency transmission and reception. In this study, a method separating these two phase contributions by comparing the electric conductivity reconstructed from different transmit channels of a parallel radiofrequency transmission system is presented. This enables specific energy absorption rate estimation not only for quadrature excitation but also for the nonquadrature excitation of the single elements of the transmit array. Though the contributions of the different phases are known, unknown magnetic field components and tissue boundary artifacts limit the technique. Nevertheless, the high agreement between simulated and experimental results found in this study is promising. B1‐based specific energy absorption rate determination might become possible for arbitrary radiofrequency excitation on a patient‐individual basis. Magn Reson Med, 2012. © 2012 Wiley Periodicals, Inc.