bluuebunny/crossref_metadata_2025_split huggingface dataset:
Association between retinal microvascular abnormalities and late-life brain amyloid-β deposition: the ARIC-PET study
Authors: Egle Marco , Deal Jennifer A. , Walker Keenan A. , Wong Dean F. , Sharrett A. Richey , Gottesman Rebecca F. 
Published: May 2024

Abstract
Background
Retinal microvascular signs are accessible measures of early alterations in microvascular dysregulation and have been associated with dementia; it is unclear if they are associated with AD (Alzheimer’s disease) pathogenesis as a potential mechanistic link. This study aimed to test the association of retinal microvascular abnormalities in mid and late life and late life cerebral amyloid.

Methods
Participants from the ARIC‐PET (Atherosclerosis Risk in Communities‐Positron Emission Tomography) study with a valid retinal measure (N = 285) were included. The associations of mid- and late-life retinal signs with late-life amyloid-β (Aβ) by florbetapir PET were tested. Two different measures of Aβ burden were included: (1) elevated amyloid (SUVR > 1.2) and (2) continuous amyloid SUVR. The retinal measures’ association with Aβ burden was assessed using logistic and robust linear regression models. A newly created retinal score, incorporating multiple markers of retinal abnormalities, was also evaluated in association with greater Aβ burden.

Results
Retinopathy in midlife (OR (95% CI) = 0.36 (0.08, 1.40)) was not significantly associated with elevated amyloid burden. In late life, retinopathy was associated with increased continuous amyloid standardized value uptake ratio (SUVR) (β (95%CI) = 0.16 (0.02, 0.32)) but not elevated amyloid burden (OR (95%CI) = 2.37 (0.66, 9.88)) when accounting for demographic, genetic and clinical risk factors. A high retinal score in late life, indicating a higher burden of retinal abnormalities, was also significantly associated with increased continuous amyloid SUVR (β (95% CI) = 0.16 (0.04, 0.32)) independent of vascular risk factors.

Conclusions
Retinopathy in late life may be an easily obtainable marker to help evaluate the mechanistic vascular pathway between retinal measures and dementia, perhaps acting via AD pathogenesis. Well-powered future studies with a greater number of retinal features and other microvascular signs are needed to test these findings.
Budget impact analysis of continuous glucose monitoring in individuals with type 2 diabetes on insulin treatment in England
Authors: Alsaif Murtada , Farhat Ali , Blumer Zoe , Barham Leela 
Published: May 2024

Abstract
Introduction
In 2022, updated guidance from NICE expanded the options for self-monitoring of blood glucose for patients with type 2 diabetes (T2DM), to include continuous glucose monitoring (CGM). In this budget impact analysis, the cost impact of CGM was compared with traditional self-monitoring of blood glucose (SMBG) in adults with T2DM over 1 year from the commissioner perspective in England.

Research Design and methods
The NICE-eligible T2DM cohort was split into 4 subgroups to enable nuanced costing by insulin administration frequency: basal human insulin, premixed insulin, basal-bolus insulin and bolus insulin. The model’s cost components comprised mild and severe hypoglycaemia (SH), diabetic ketoacidosis (DKA), consumables and healthcare resource utilisation in primary and secondary care.

Results
The introduction of CGM is estimated to be cost additive by approximately £4.6 million in the basecase, driven by increased spending on the CGM device. Overall, healthcare activity was reduced by approximately 20,000 attendances, due to fewer SH and DKA episodes in the CGM arm. General Practitioner (GP) practice-based activity is expected to drop after the first year as patients requiring CGM training is reduced. The budget impact could be neutralised if the CGM sensor was discounted by 13.2% (£29.76 to £25.83).

Conclusions
CGM may result in increased spending in the NICE-eligible T2DM cohort but is expected to reduce demand on secondary care services and GP time. These findings may be of interest to local decision-makers who wish to resolve the COVID-19 backlog with transformational investment in primary care to reduce secondary care activity.
On ultrametrics, b-metrics, w-distances, metric-preserving functions, and fixed point theorems
Authors: Samphavat Suchat , Prinyasart Thanakorn 
Published: May 2024

AbstractIn this article, new classes of functions based on new variations of metric-preserving functions are defined. Necessary and sufficient conditions for functions to be in these classes are also provided. As a result, we can explain relations between all classes and learn that all functions in the classes are weakly separated from 0. We can extend fixed point theorems, which were originally provided by Kirk and Shahzad and were later extended by Pongsriiam and Termwuttipong, in this journal by considering all functions that are weakly separated from 0.
Decreasing the energy demand in public buildings using nature-based solutions: case studies from Novi Sad (Republic of Serbia) and Osijek (Republic of Croatia)
Authors: Savić Stevan , Krstić Hrvoje , Šećerov Ivan , Dunjić Jelena 
Published: May 2024

Abstract
Background
Nature-based solutions (NBS) in urban areas offer an opportunity to improve environmental conditions and to reduce CO2 emissions towards establishing climate-neutral cities in the next few decades. Furthermore, the implementation of NBSs—vertical or horizontal green infrastructures on public facilities—could in particular improve both climate, including outdoor thermal conditions on a micro-scale (especially during the summer season) and the energy demand of buildings as well as save heating energy during the winter period.

Results
On both selected buildings, extensive green roofs were implemented as an NBS intervention. The analysed data were obtained using the monitoring systems (from 2019 to 2022) installed on two public buildings in Novi Sad (Republic of Serbia) and Osijek (Republic of Croatia), with a focus on climate/bioclimate characteristics and thermal transmission capacities. Four automatic weather stations (AWS) were used for microclimate monitoring, along with the heat flow meter (HFM) method, to measure the alterations in the thermal transmittance (U value) of a flat concrete roof before and after energy refurbishment and the installation of a green roof. The outcomes of this study show that the air temperatures (Ta) and globe temperatures (Tg) near the green roof are lower by 0–3 °C for Ta and by 0–16.5 °C for Tg than the values captured by the AWSs at other locations. An even more interesting fact is that the green roof has a constant cooling potential during tropical nights, and based upon this research, the cooling value is around 2 °C for Tg (the Ta value is not distinct). The thermal transmittance results show that more savings can be achieved by applying a green roof with an 8 cm thick substrate: U values decreased by 50–69%, as measured by two different heat flux sensors.

Conclusions
Nature-based solutions, such as the implementation of an extensive green roof, have positive effects on diverse aspects of urban environments and building energy savings, which are particularly evident in extreme seasons, both summer and winter. Applying the proposed monitoring and assessment system could help local communities in their efforts to reduce carbon-based emissions. This paper provides a good example of the implementation of NBSs on a local- and a micro-scale.
Psychophysiological effects of walking in forests and urban built environments with disparate road traffic noise exposure: study protocol of a randomized controlled trial
Authors: Schaupp Julia , Hediger Karin , Wunderli Jean-Marc , Schäffer Beat , Tobias Silvia , Kolecka Natalia , Bauer Nicole 
Published: May 2024

Abstract
Background
Stress is a widespread phenomenon and reality of everyday life, entailing negative consequences for physical and psychological wellbeing. Previous studies have indicated that exposure to greenspaces and nature-based interventions are promising approaches to reducing stress and promoting restoration. However, an increasing percentage of the population lives in urban regions with limited opportunities to spend time in greenspaces. In addition, urban settings typically feature increased levels of noise, which represents a major environmental stressor. Although various studies have compared the effects of exposure to greenspaces versus urban built environments, evidence of the effects of noise in this context is very limited. Psychophysiological benefits of exposure to greenspaces compared to urban built environments reported in earlier studies might be less (or at least not only) due to features of the greenspaces than to additional stressors, such as road traffic noise in the urban built environment. Hence, differences in the effects attributed to greenness in previous studies may also be due to potentially detrimental noise effects in comparison settings. This paper reports the study protocol for a randomized, controlled intervention study comparing the effects of walking in forest versus urban built environments, taking road traffic noise exposure during walks in the respective settings into account.

Methods
The protocol envisages a field study employing a pretest–posttest design to compare the effects of 30-min walks in urban built environments and forests with different road traffic noise levels. Assessments will consist of self-reported measures, physiological data (salivary cortisol and skin conductance), an attention test, and noise, as well as greenness measurements. The outcomes will be restoration, stress, positive and negative affect, attention, rumination, and nature connectedness.

Discussion
The results will inform about the restorative effect of walking in general, of exposure to different types of environments, and to different noise levels in these sites. The study will provide insights into the benefits of walking and nature-based interventions, taking into account the potential detrimental effects of noise exposure. It will thus facilitate a better understanding of low-threshold interventions to prevent stress and foster wellbeing.

Trial registration
ISRCTN48943261; Registered 23.11.2023.
Validation of an elicited Imitation test as a measure of Korean language proficiency
Authors: Kim Hojung , Song Changkyung , Kim Jiyoung , Jeong Hyeyun , Park Jisoo 
Published: May 2024

AbstractThis study presents a modified version of the Korean Elicited Imitation (EI) test, designed to resemble natural spoken language, and validates its reliability as a measure of proficiency. The study assesses the correlation between average test scores and Test of Proficiency in Korean (TOPIK) levels, examining score distributions among beginner, intermediate, and advanced learner groups. Using item response theory (IRT), the study explores the influence of four key facets—learners, items, raters, and constructs—on performance evaluation. An explanatory item response modeling (EIRM) analysis identified linguistic factors impacting the EI test’s performance. Notably, the study uncovered a robust positive correlation between EI test scores and TOPIK levels. Significant score disparities were observed between beginner and intermediate, as well as beginner and advanced, learner cohorts. An IRT-based exploration of each facet revealed that item difficulty was comparatively lower in contrast to learners’ commendable performance, and raters exhibited a high degree of scoring consistency. The EIRM analysis underscores the significance of variables such as the number of syllables, vocabulary score, and content word density in influencing test performance.
Comprehensive analysis of the REST transcription factor regulatory networks in IDH mutant and IDH wild-type glioma cell lines and tumors
Authors: Perycz Malgorzata , Dabrowski Michal J. , Jardanowska-Kotuniak Marta , Roura Adria-Jaume , Gielniewski Bartlomiej , Stepniak Karolina , Dramiński Michał , Ciechomska Iwona A. , Kaminska Bozena , Wojtas Bartosz 
Published: May 2024

AbstractThe RE1-silencing transcription factor (REST) acts either as a repressor or activator of transcription depending on the genomic and cellular context. REST is a key player in brain cell differentiation by inducing chromatin modifications, including DNA methylation, in a proximity of its binding sites. Its dysfunction may contribute to oncogenesis. Mutations in IDH1/2 significantly change the epigenome contributing to blockade of cell differentiation and glioma development. We aimed at defining how REST modulates gene activation and repression in the context of the IDH mutation-related phenotype in gliomas. We studied the effects of REST knockdown, genome wide occurrence of REST binding sites, and DNA methylation of REST motifs in IDH wild type and IDH mutant gliomas. We found that REST target genes, REST binding patterns, and TF motif occurrence proximal to REST binding sites differed in IDH wild-type and mutant gliomas. Among differentially expressed REST targets were genes involved in glial cell differentiation and extracellular matrix organization, some of which were differentially methylated at promoters or gene bodies. REST knockdown differently impacted invasion of the parental or IDH1 mutant glioma cells. The canonical REST-repressed gene targets showed significant correlation with the GBM NPC-like cellular state. Interestingly, results of REST or KAISO silencing suggested the interplay between these TFs in regulation of REST-activated and repressed targets. The identified gene regulatory networks and putative REST cooperativity with other TFs, such as KAISO, show distinct REST target regulatory networks in IDH-WT and IDH-MUT gliomas, without concomitant DNA methylation changes. We conclude that REST could be an important therapeutic target in gliomas.
Reciprocal relationship between self-efficacy and achievement in mathematics among high school students
Authors: Liu Ruixue , Jong Cindy , Fan Meng 
Published: May 2024

AbstractSelf-efficacy or perceived ability refers to the confidence an individual has in their ability to successfully perform a specific task. Previous researchers have demonstrated the importance of self-efficacy beliefs and its impact on predicting students’ mathematics performances. Specifically, gender has been regarded as an important moderator variable in the prediction of achievement from measures of affect. To this end, using data from High School Longitudinal Study of 2009, this study utilized the multi-group analysis under the framework of structural equation modeling to examine gender differences in the reciprocal relationship between mathematics self-efficacy and mathematics achievement. The results showed that there were statistically significant differences between male students and female students in the reciprocal relationship between mathematics self-efficacy and mathematics achievement; the impact of prior mathematics achievement on later mathematics achievement was stronger than the impact of prior mathematics self-efficacy on later mathematics self-efficacy. Significance of these findings were discussed in this paper.
Rapid development and mass production of SARS-CoV-2 neutralizing chicken egg yolk antibodies with protective efficacy in hamsters
Authors: Zhao Binan , Peng Haoran , Zhang Yanjing , Zhang Jie , Kong Desheng , Cao Sai , Li Yan , Yang Dan , Sun Chuanwen , Pu Xinyi , Zhao Ping , Xu Yan , Zhao Kai , Xie Liangzhi 
Published: May 2024

AbstractDespite the record speed of developing vaccines and therapeutics against the SARS-CoV-2 virus, it is not a given that such success can be secured in future pandemics. In addition, COVID-19 vaccination and application of therapeutics remain low in developing countries. Rapid and low cost mass production of antiviral IgY antibodies could be an attractive alternative or complementary option for vaccine and therapeutic development. In this article, we rapidly produced SARS-CoV-2 antigens, immunized hens and purified IgY antibodies in 2 months after the SARS-CoV-2 gene sequence became public. We further demonstrated that the IgY antibodies competitively block RBD binding to ACE2, neutralize authentic SARS-CoV-2 virus and effectively protect hamsters from SARS-CoV-2 challenge by preventing weight loss and lung pathology, representing the first comprehensive study with IgY antibodies. The process of mass production can be easily implemented in most developing countries and hence could become a new vital option in our toolbox for combating viral pandemics. This study could stimulate further studies, optimization and potential applications of IgY antibodies as therapeutics and prophylactics for human and animals.
The effectiveness of cervical mucus electrical impedance compared to basal body temperature to determine fertility window
Authors: Tabbaa Suzanne , Hambright Sealy , Sikes Katie J. , Levy Gary , Rydfors Jan 
Published: May 2024

Abstract
Background
Serial serum hormone measurements and transvaginal ultrasound are reliable measures to predict ovulation. These measures are inconvenient and expensive therefore, basal body temperature charting (BBT) and urine ovulation predictor kits (OPK) for luteinizing hormone are often used to determine the 6-day fertile window. However, BBT does not clearly change until 1–2 days after ovulation. Additionally, while OPK can indicate positivity prior to ovulation, false readings are common. A novel alternative approach involves measuring electrolyte trends in cervical mucus using electrical impedance spectroscopy. Cervical mucus electrolyte measurements are associated with hormone level changes during the menstrual cycle. The purpose of this study was to compare the effectiveness of cervical mucus electrical impedance and basal body temperature. We sought to determine if cervical mucus electrolyte measurements provided improved detection of the ovulation day and therefore, improve fertility timing for women.

Methods
14 healthy women between 18 and 44 years of age with normal menstrual cycles were enrolled in the Observational Study. Participants measured BBT and cervical mucus electrical impedance daily for 3 menstrual cycles using Kegg (Lady Technologies Inc. San Francisco, California, USA). Ovulation date for each cycle was confirmed by measuring hormone levels in urine and serum, and by vaginal ultrasound.

Results
Electrical impedance was significantly different between the follicular phase versus ovulatory date (p = 0.007) and between the luteal phase versus the ovulatory date (p = 0.007). A significant difference in the rate of change of cervical impedance measurements in the pre-ovulatory follicular phase was found compared to BBT (p = 0.0225). The sensitivity (+ 7.14%), specificity (+ 20.35%), and accuracy (+ 17.59) to determine the 1-day fertility window was significantly higher using cervical mucus impedance compared to BBT.

Conclusions
BBT is considered unreliable for evaluating ovulatory function. Cervical mucus electrical impedance offers a novel measure of electrolyte changes associated with hormone levels. We report that pre-ovulatory electrical impedance patterns demonstrated higher sensitivity, specificity, and accuracy for determining the fertility window when compared to BBT. These findings suggest that changes in electrical impedance may provide an accurate method for predicting ovulation and for measuring ovulatory function.
Prospective memory instruments for the assessment of children and adolescents: a systematic review
Authors: Signori Vanessa de A. , Watanabe Tiago M. , de Pereira Ana Paula A. 
Published: May 2024

Abstract
Background
Prospective memory is the ability to engage in an intention to be performed in the future. The main objective of this study was to identify instruments that assess both time-based and event-based prospective memory in children and adolescents and that have the potential to be clinically applicable.

Method
Three databases (PubMed, Scopus, and PsycINFO) were searched to identify existing PM measures in original articles published until 2022. Literature searches were conducted using the following terms: (prospective memor* OR memor* for intentions) AND (neuropsychological assessment) AND (test* OR instrument* OR questionnaire* OR task*) AND (psychometric properties) AND (child* OR adolescen*). Relevant studies identified in the reference lists were also included in the review.

Results
Ten instruments were identified and classified into three categories: (a) test batteries, (b) experimental procedures, and (c) questionnaires. All the instruments identified were described concerning their content and the psychometric properties available. Some of the instruments presented empirical evidence regarding validity and reliability, but no one provided normative data.

Conclusion
Besides the recent progress regarding studies publishing the development of a variety of novel measures, there are still many limitations surrounding the assessment of PM in the youth population because of the yet incipient psychometric properties presented by the majority of the PM instruments. Recommendations for a gold-standard PM instrument for assessing children and adolescents are provided.
Reproducibility of a semiautomatic lobar lung tissue assignment technique on noncontrast CT scans: a study on swine animal model
Authors: Luu Nile , Van Nathan , Shojazadeh Alireza , Zhao Yixiao , Molloi Sabee 
Published: May 2024

Abstract
Background
To evaluate the reproducibility of a vessel-specific minimum cost path (MCP) technique used for lobar segmentation on noncontrast computed tomography (CT).

Methods
Sixteen Yorkshire swine (49.9 ± 4.7 kg, mean ± standard deviation) underwent a total of 46 noncontrast helical CT scans from November 2020 to May 2022 using a 320-slice scanner. A semiautomatic algorithm was employed by three readers to segment the lung tissue and pulmonary arterial tree. The centerline of the arterial tree was extracted and partitioned into six subtrees for lobar assignment. The MCP technique was implemented to assign lobar territories by assigning lung tissue voxels to the nearest arterial tree segment. MCP-derived lobar mass and volume were then compared between two acquisitions, using linear regression, root mean square error (RMSE), and paired sample t-tests. An interobserver and intraobserver analysis of the lobar measurements was also performed.

Results
The average whole lung mass and volume was 663.7 ± 103.7 g and 1,444.22 ± 309.1 mL, respectively. The lobar mass measurements from the initial (MLobe1) and subsequent (MLobe2) acquisitions were correlated by MLobe1 = 0.99 MLobe2 + 1.76 (r = 0.99, p = 0.120, RMSE = 7.99 g). The lobar volume measurements from the initial (VLobe1) and subsequent (VLobe2) acquisitions were correlated by VLobe1 = 0.98VLobe2 + 2.66 (r = 0.99, p = 0.160, RSME = 15.26 mL).

Conclusions
The lobar mass and volume measurements showed excellent reproducibility through a vessel-specific assignment technique. This technique may serve for automated lung lobar segmentation, facilitating clinical regional pulmonary analysis.

Relevance statement
Assessment of lobar mass or volume in the lung lobes using noncontrast CT may allow for efficient region-specific treatment strategies for diseases such as pulmonary embolism and chronic thromboembolic pulmonary hypertension.

Key points
• Lobar segmentation is essential for precise disease assessment and treatment planning.
• Current methods for segmentation using fissure lines are problematic.
• The minimum-cost-path technique here is proposed and a swine model showed excellent reproducibility for lobar mass measurements.
• Interobserver agreement was excellent, with intraclass correlation coefficients greater than 0.90.

Graphical Abstract