bluuebunny/crossref_metadata_2025_split huggingface dataset:
Caenorhabditis elegans TRPV Channels Function in a Modality-Specific Pathway to Regulate Response to Aberrant Sensory Signaling
Authors: Ezak Meredith J , Hong Elizabeth , Chaparro-Garcia Angela , Ferkey Denise M 
Published: February 2010

Abstract
Olfaction and some forms of taste (including bitter) are mediated by G protein-coupled signal transduction pathways. Olfactory and gustatory ligands bind to chemosensory G protein-coupled receptors (GPCRs) in specialized sensory cells to activate intracellular signal transduction cascades. G protein-coupled receptor kinases (GRKs) are negative regulators of signaling that specifically phosphorylate activated GPCRs to terminate signaling. Although loss of GRK function usually results in enhanced cellular signaling, Caenorhabditis elegans lacking GRK-2 function are not hypersensitive to chemosensory stimuli. Instead, grk-2 mutant animals do not chemotax toward attractive olfactory stimuli or avoid aversive tastes and smells. We show here that loss-of-function mutations in the transient receptor potential vanilloid (TRPV) channels OSM-9 and OCR-2 selectively restore grk-2 behavioral avoidance of bitter tastants, revealing modality-specific mechanisms for TRPV channel function in the regulation of C. elegans chemosensation. Additionally, a single amino acid point mutation in OCR-2 that disrupts TRPV channel-mediated gene expression, but does not decrease channel function in chemosensory primary signal transduction, also restores grk-2 bitter taste avoidance. Thus, loss of GRK-2 function may lead to changes in gene expression, via OSM-9/OCR-2, to selectively alter the levels of signaling components that transduce or regulate bitter taste responses. Our results suggest a novel mechanism and multiple modality-specific pathways that sensory cells employ in response to aberrant signal transduction.
Chronic pain 5 years after randomized comparison of laparoscopic and Lichtenstein inguinal hernia repair
Authors:   Swedish Multicentre Trial of Inguinal Hernia Repair by Laparoscopy (SMIL) study group, Eklund A , Montgomery A , Bergkvist L , Rudberg C 
Published: February 2010

Abstract

Background
Chronic postoperative pain is a major drawback of inguinal hernia repair. This study compared the frequency of chronic pain after laparoscopic (totally extraperitoneal patch, TEP) and open (Lichtenstein) repairs.

Methods
A randomized multicentre study with 5 years' follow-up was conducted on men with a primary inguinal hernia. Chronic pain was categorized as mild, moderate or severe by blinded observers. A subgroup analysis was performed on 121 patients who experienced moderate or severe pain at any time during follow-up.

Results
Overall, 1370 of 1512 randomized patients underwent surgery, 665 in the TEP and 705 in the Lichtenstein group. The total incidence of chronic pain was 11·0 versus 21·7 per cent at 1 year, 11·0 versus 24·8 per cent at 2 years, 9·9 versus 20·2 per cent at 3 years and 9·4 versus 18·8 per cent at 5 years in the TEP and Lichtenstein groups respectively (P &lt; 0·001). After 5 years, 1·9 per cent of patients in the TEP and 3·5 per cent in the Lichtenstein group reported moderate or severe pain (P = 0·092). Of the 121 patients, 72 (59·5 per cent) no longer reported pain a median of 9·4 (range 6·7–10·8) years after operation.

Conclusion
Five years after surgery only a small proportion of patients still report moderate to severe chronic pain. Laparoscopic inguinal hernia repair leads to less chronic pain than open repair. Registration number: NCT00568269 (http://www.clinicaltrials.gov).
Coughing‐induced stress cardiomyopathy
Authors: Butman Samuel M. 
Published: February 2010

AbstractStress cardiomyopathy is described as acute myocardial infarction provoked by a stressful event with evidence of a significant focal wall motion but with little or no significant coronary artery disease. In this case report, a particularly bad bout of coughing resulted in chest pressure, troponin release, and evidence of antero‐apical dyskinesis despite angiographic normal coronary arteries. The patient made a full recovery of function after an uncomplicated hospital stay. © 2010 Wiley‐Liss, Inc.
Carotid artery stenting in octogenarians using a proximal endovascular occlusion cerebral protection device: A multicenter registry
Authors: Micari Antonio , Stabile Eugenio , Cremonesi Alberto , Vadalà Giuseppe , Castriota Fausto , Pernice Vincenzo , Sorropago Giovanni , Rubino Paolo , Biamino Giancarlo 
Published: February 2010

AbstractBackground: Carotid stenting (CAS) has been proposed as an alternative to carotid endoarterectomy also in elderly patients with discrepant results. However, the use of proximal neuroprotection devices have not been evaluated in octogenarians. Purpose: The aim of this multicenter prospective registry was to demonstrate that CAS in octogenarians is safe and effective if performed in high‐volume centers by experienced operators. Methods: From July 2005 to May 2009, a total of 198 octogenarians patients, in three different institutions, were included in this registry. All patients underwent CAS using proximal endovascular occlusion device (Mo.Ma. device Invatec, Roncadelle, Italy). An independent neurologist evaluated all patients. The primary endpoint was death and stroke rate at 30 days. Results: 198 octogenarians (135 men; mean age: 83.2 years) were included in the registry. 39.4% of the patients were symptomatic. Procedural success was 100%. In‐hospital complications: Two minor and two major strokes (2.02%) occurred. No device‐related complications and no serious access site complication were noted. Between discharge and 30‐day follow‐up, one patient died due to a cardiac arrest. The overall 30‐day combined stroke/death rate was 2.52%, resulting in 1.61% event incidence in asymptomatic and 3.9% in symptomatic patients (P = ns). Logistic regression did not identify independent predictor of neurological events, except in the female gender. Conclusion: This multicenter prospective registry shows that CAS performed with proximal flow blockage is safe and feasible also in octogenarians. Thirty days death/stroke rates are similar to those of the overall population and within the International guidelines. © 2010 Wiley‐Liss, Inc.
Clinical Outcome Following Conservative vs Revascularization Therapy in Patients With Stable Coronary Artery Disease and Borderline Fractional Flow Reserve Measurements
Authors: Lindstaedt Michael , Halilcavusogullari Yasemin , Yazar Aydan , Holland‐Letz Tim , Bojara Waldemar , Mügge Andreas , Germing Alfried 
Published: February 2010

AbstractBackgroundFractional flow reserve (FFR) measurements in the so‐called gray‐zone range of ≥ 0.75 and ⩽0.80 are associated with uncertainty concerning the guidance of patient therapy. It is unclear whether any difference in clinical outcome exists when revascularization treatment of FFR‐evaluated lesions in this borderline range is deferred or performed. The objective of this study is to compare the clinical outcome of these patients with respect to their recommended treatment strategy.MethodsOut of a single center database of 900 consecutive patients with stable coronary artery disease, 97 patients with borderline FFR measurements were identified and included in the study. The rate of major adverse cardiac events (MACE; cardiac death, myocardial infarction (MI), coronary revascularization) and the presence of angina were evaluated at follow‐up.ResultsA total of 48 patients were deferred from revascularization and 49 patients underwent revascularization. There was no difference in risk profile between these groups. At a mean follow‐up of 24±16 months, event‐free survival in the deferred group was significantly better regarding overall MACE, combined rate of cardiac death, and MI, as well as MACE related to the FFR‐evaluated vessel. No difference with regard to the presence of angina was observed.ConclusionsPatients with coronary lesions in the borderline FFR range can be deferred from revasculariza‐tion without putting them at increased risk for major adverse events. Revascularization may be considered in the course of therapy on an individual basis if typical angina persists or worsens despite maximal medical treatment. Copyright © 2010 Wiley Periodicals, Inc.
Difference in TB10.4 T‐cell epitope recognition following immunization with recombinant TB10.4, BCG or infection with Mycobacterium tuberculosis
Authors: Billeskov Rolf , Grandal Michael V. , Poulsen Christian , Christensen Jan P. , Winter Nathalie , Vingsbo‐Lundberg Carina , Hoang Truc T. K. T. , van Deurs Bo , Song Young‐Hwa , Aagaard Claus , Andersen Peter , Dietrich Jes 
Published: February 2010

AbstractMost novel vaccines against infectious diseases are based on recombinant Ag; however, only few studies have compared Ag‐specific immune responses induced by natural infection with that induced by the same Ag in a recombinant form. Here, we studied the epitope recognition pattern of the tuberculosis vaccine Ag, TB10.4, in a recombinant form, or when expressed by the pathogen Mycobacterium tuberculosis (M.tb), or by the current anti‐tuberculosis vaccine, Mycobacterium bovis BCG. We showed that BCG and M.tb induced a similar CD4+ T‐cell specific TB10.4 epitope‐pattern, which differed completely from that induced by recombinant TB10.4. This difference was not due to post‐translational modifications of TB10.4 or because TB10.4 is secreted from BCG and M.tb as a complex with Rv0287. In addition, BCG and TB10.4/CAF01 were both taken up by DC and macrophages in vivo, and in vitro uptake experiments revealed that both TB10.4 and BCG were transported to Lamp+‐compartments. BCG and TB10.4 however, were directed to different types of Lamp+‐compartments in the same APC, which may lead to different epitope recognition patterns. In conclusion, we show that different vectors can induce completely different recognition of the same protein.
Organometallic Synthesis of β‐CoAl Nanoparticles and β‐CoAl/Al Nanoparticles and Their Behaviour upon Air Exposure
Authors: Cokoja Mirza , Parala Harish , Birkner Alexander , Fischer Roland A. , Margeat Olivier , Ciuculescu Diana , Amiens Catherine , Chaudret Bruno , Falqui Andrea , Lecante Pierre 
Published: February 2010

AbstractNanocolloids of the intermetallic β‐CoAl phase were prepared by a soft organometallic route. They were fully characterized by (HR)TEM, EDX, WAXS, XAS and SQUID magnetometry. Their exposure to air led to an increased saturation magnetization in agreement with Co/Al segregation and the formation of Co/Al2O3 nanocomposite. Furthermore, the β‐CoAl nanoparticles could be used as seeds to grow an aluminum overlayer, which passivated the alloyed core against oxidation. These nanoparticles yielded stable colloidal solutions in aromatic solvents.
Consumer price behaviour: evidence from Luxembourg micro data
Authors: Lünnemann Patrick , Mathä Thomas Y. 
Published: February 2010

AbstractWe analyse micro‐level consumer price data in Luxembourg with a particular view on price change reversals and wage indexation. The median duration is roughly 8 months. On an average, price decreases are as large as price increases. With the exception of services, individual prices do not show signs of downward rigidity. Excluding price change reversals reduces the weighted frequency of price change from 17 to 12%. Accumulated price and wage inflation, automatic wage indexation, and the cash changeover increase the probability of a price change, whereas pricing at attractive pricing points and price regulation have the opposite effect. Copyright © 2010 John Wiley & Sons, Ltd.
Environmental risk factors of West Nile virus infection of horses in the Senegal River basin
Authors: CHEVALIER V. , DUPRESSOIR A. , TRAN A. , DIOP O. M. , GOTTLAND C. , DIALLO M. , ETTER E. , NDIAYE M. , GROSBOIS V. , DIA M. , GAIDET N. , SALL A. A. , SOTI V. , NIANG M. 
Published: February 2010

SUMMARYIn 2005, a serological study was carried out on horses in five ecologically contrasted zones of the Senegal River basin (Senegal) to assess West Nile virus (WNV) transmission and investigate underlying environmental risk factors. In each study zone, horses were randomly selected and blood samples taken. A land-cover map of the five study areas was built using two satellite ETM+ images. Blood samples were screened by ELISA for anti-WNV IgM and IgG and positive samples were confirmed by seroneutralization. Environmental data were analysed using a principal components analysis. The overall IgG seroprevalence rate was 85% (n=367; 95% CI 0·81–0·89). The proximity to sea water, flooded banks and salted mudflats were identified as protective factors. These environmental components are unfavourable to the presence of Culex mosquitoes suggesting that in Senegal, the distribution of the vector species is more limiting for WNV transmission than for the hosts' distribution.
GO-Bayes: Gene Ontology-based overrepresentation analysis using a Bayesian approach
Authors: Zhang Song , Cao Jing , Kong Y. Megan , Scheuermann Richard H. 
Published: February 2010

Abstract
Motivation: A typical approach for the interpretation of high-throughput experiments, such as gene expression microarrays, is to produce groups of genes based on certain criteria (e.g. genes that are differentially expressed). To gain more mechanistic insights into the underlying biology, overrepresentation analysis (ORA) is often conducted to investigate whether gene sets associated with particular biological functions, for example, as represented by Gene Ontology (GO) annotations, are statistically overrepresented in the identified gene groups. However, the standard ORA, which is based on the hypergeometric test, analyzes each GO term in isolation and does not take into account the dependence structure of the GO-term hierarchy.
Results: We have developed a Bayesian approach (GO-Bayes) to measure overrepresentation of GO terms that incorporates the GO dependence structure by taking into account evidence not only from individual GO terms, but also from their related terms (i.e. parents, children, siblings, etc.). The Bayesian framework borrows information across related GO terms to strengthen the detection of overrepresentation signals. As a result, this method tends to identify sets of closely related GO terms rather than individual isolated GO terms. The advantage of the GO-Bayes approach is demonstrated with a simulation study and an application example.
Contact: song.zhang@utsouthwestern.edu; richard.scheuermann@utsouthwestern.edu
Supplementary information: Supplementary data are available at Bioinformatics online.
Retroperitoneoscopic live‐donor nephrectomy: 5‐year single‐center experience in China
Authors: Ma Lulin , Ye Jianfei , Huang Yi , Hou Xiaofei , Zhao Lei , Wang Guoliang 
Published: February 2010

Objective:  To evaluate the efficacy and the learning‐curve period of our modified retroperitoneoscopic live‐donor nephrectomy.Methods:  From December 2003 to May 2009, 138 consecutive retroperitoneoscopic live‐donor nephrectomies were carried out at our institution. Donors were separated into four groups in consecutive sequence in order to determine the learning‐curve period. Groups 1–3 included forty consecutive cases each, whereas group 4 included the last eighteen cases. The renal artery and vein were controlled with two plastic locking clips at proximal ends without any clips on the kidney side. The kidney was manually retrieved through lumbar incision.Results:  Mean operative times were 160.5, 116.9, 101.4 and 109.2 min in groups 1–4, respectively (group 1 vs group 2, 3 or 4, P < 0.01). Mean warm ischemic time was 3.5 min. Mean estimated blood loss was 88.8, 73.0, 69.3 and 43.9 mL in groups 1–4, respectively (group 1 vs group 4, P < 0.01). No blood transfusion or open conversion was required. Mean hospital stay was 7.8, 6.9, 6.6 and 5.8 days in groups 1–4, respectively (group 1 vs group 4, P < 0.05). Eight donors and seven grafts suffered from complications. Complication rates were 22.5%, 7.5%, 5.0% and 6.0% in groups 1–4, respectively (group 1 vs group 3, P < 0.05).Conclusion:  Our modified retroperitoneoscopic live‐donor nephrectomy can be carried out safely with a learning‐curve period of about 40 cases.
Perioperative anxiety in children
Authors: FORTIER MICHELLE A. , DEL ROSARIO ANTONIO M. , MARTIN SARAH R. , KAIN ZEEV N. 
Published: February 2010

SummaryObjectives & Aim:  The purpose of this investigation was to examine children’s anxiety across the perioperative setting.Background:  Although several studies have examined preoperative anxiety in children, few researchers have illustrated perioperative anxiety; that is, anxiety in children throughout the pre and postoperative continuum.Methods:  Participants were 261 children ages 2–12. Anxiety was rated prior to surgery, immediately after surgery, and for 2 weeks at home following surgery.Results:  Low child sociability and high parent anxiety predicted perioperative anxiety. Perioperative anxiety was related to postoperative pain and negative postoperative behavioral change.Conclusions:  Identification and prevention of anxiety in children can help prevent negative outcomes following surgery.
ROBERT JOHN RUSSELL VERSUS THE NEW ATHEISTS
Authors: Murphy Nancey 
Published: February 2010

Abstract.This essay compares Robert John Russell's work in his recent bookCosmology from Alpha to Omega: The Creative Mutual Interaction of Theology and Science(2008) to that of the authors known collectively as “the new atheists.” I treat the latter as recent contributors to the modern tradition of scientific naturalism. This tradition makes claims to legitimacy on the basis of its close relations to the natural sciences. The purpose of this essay is to show up the poverty of the naturalist tradition's scientific credentials by contrasting it with Russell's careful account of positive relations between science and Christian theology.
EMBODIED SCIENCE: RECENTERING RELIGION‐AND‐SCIENCE
Authors: Hefner Philip 
Published: February 2010

Abstract.Neither religion nor science is first of all a realm of pure ideas, even though religion‐and‐science discussions often assume that they are. I propose that a concept of embodied science is more adequate and that religion‐and‐science should center its attention on science as enabler for improving the world (SEIW). This idea of science is rooted in Jerome Ravetz's concept of industrialized science and Donna Haraway's technoscience. SEIW describes the sociocultural context of science in commercial, government, and university settings. The chief focus of religion‐and‐science consequently takes into account five basic issues: (1) the kind of world we want, (2) liberating science, (3) human action and ethics, (4) religion and the world's possibilities, and (5) recovering myth. An underlying presupposition of the discussion is that understanding the world always involves as well an understanding of our being‐in‐the‐world.
EDITORIAL
Authors: Davenport Anthony P , Daly Craig 
Published: February 2010

This themed section on Imaging in Pharmacology comprising reviews and original articles arose from two symposia held at the Summer Meeting of the British Pharmacological Society in Edinburgh 8–10 July 2009 on Developments in Receptor Imaging and Imaging and Targeting Inflammation in Stroke and Atherosclerosis. The reports cover a broad spectrum of pharmacological studies, from whole animal imaging to gene expression and emphasize the importance of imaging techniques in pharmacology. The development of each new imaging methodology brings pharmacology closer to the ambitious goal of being able to image (simultaneously) each component part of the G‐protein‐coupled receptor signalling process.This article is part of a themed section on Imaging in Pharmacology. See the reference list for all papers appearing in this section.
Knowledge Life Cycle, Knowledge Inventory, and Knowledge Acquisition Strategies*
Authors: Chen Andrew N. K. , Hwang Yuhchang , Raghu T. S. 
Published: February 2010

ABSTRACTFor a knowledge‐ and skill‐centric organization, the process of knowledge management encompasses three important and closely related elements: (i) task assignments, (ii) knowledge acquisition through training, and (iii) maintaining a proper level of knowledge inventory among the existing workforce. Trade‐off on choices between profit maximization in the short run and agility and flexibility in the long term is a vexing problem in knowledge management. In this study, we examine the effects of different training strategies on short‐term operational efficiency and long‐term workforce flexibility. We address our research objective by developing a computational model for task and training assignment in a dynamic knowledge environment consisting of multiple distinct knowledge dimensions. Overall, we find that organizational slack is an important variable in determining the effectiveness of training strategies. Training strategies focused on the most recent skills are found to be the preferred option in most of the considered scenarios. Interestingly, increased efficiencies in training can actually create preference conflict between employees and the firm. Our findings indicate that firms facing longer knowledge life cycles, higher slack in workforce capacity, and better training efficiencies actually face more difficult challenges in knowledge management.
Organizational Integration for Product Development: The Effects on Glitches, On‐Time Execution of Engineering Change Orders, and Market Success
Authors: Koufteros Xenophon A. , Rawski Greg E. , Rupak Rauniar 
Published: February 2010

ABSTRACTDeviations from requirements during the product development process can be considered as glitches. Fixing glitches, or problems, during the product development process consumes valuable resources, which may adversely affect product development time and hamper the firm's goal to pursue a first‐mover advantage. It is posited that an integrated organizational response can diminish incidences of glitches and improve the ability of the firm to respond to engineering changes, subsequently leading to improved market success. This organizational response frequently includes heavyweight product development managers who are seen as essential catalysts for internal integration. Though internal integration is vital, it is equally important to integrate with customers and suppliers alike because such network partners can provide access to information, knowledge, and unique and complementary resources that are otherwise unavailable to the firm. Findings, which are based on a sample of 191 product development projects in the automotive industry, suggest that some integration routines have a positive impact on product development outcomes and market success, while other routines can in fact hamper the collective effort.