bluuebunny/crossref_metadata_2025_split huggingface dataset:
Two new mutations in the HIF2A gene associated with erythrocytosis
Authors: Percy Melanie J. , Chung Yu Jin , Harrison Claire , Mercieca Jane , Hoffbrand A. Victor , Dinardo Carla L. , Santos Paulo C.J.L. , Fonseca Guilherme H.H. , Gualandro Sandra F.M. , Pereira Alexandre C. , Lappin Terence R.J. , McMullin Mary Frances , Lee Frank S. 
Published: January 2012

AbstractCongenital or familial erythrocytosis/polycythemia can have many causes, and an emerging cause is genetic disruption of the oxygen‐sensing pathway that regulates the Erythropoietin (EPO) gene. More specifically, recent studies have identified erythrocytosis‐associated mutations in the HIF2A gene, which encodes for Hypoxia Inducible Factor‐2α (HIF‐2α), as well as in two genes that encode for proteins that regulate it, Prolyl Hydroxylase Domain protein 2 (PHD2) and the von Hippel Lindau tumor suppressor protein (VHL). We report here the identification of two new heterozygous HIF2A missense mutations, M535T, and F540L, both associated with erythrocytosis. Met‐535 has previously been identified as a residue mutated in other patients with erythrocytosis; although, the mutation of this particular residue to Thr has not been reported. In contrast, Phe‐540 has not been reported as a residue mutated in erythrocytosis, and we present evidence here that this mutation impairs interaction of HIF‐2α with both VHL and PHD2. Am. J. Hematol. 2012. © 2012 Wiley Periodicals, Inc.
Approaches to investigating common bleeding disorders: An evaluation of North American coagulation laboratory practices
Authors: Hayward Catherine P.M. , Moffat Karen A. , Plumhoff Elizabeth , Van Cott Elizabeth M. 
Published: January 2012

AbstractBleeding disorders commonly result from deficiencies or defects in von Willebrand factor (VWF), platelets, coagulation factors, or fibrinolytic proteins. The primary goal of our study was to assess current North American coagulation laboratory practices for diagnosing bleeding disorders, using an on‐line patterns‐of‐practice survey of diagnostic laboratory members of the North American Specialized Coagulation Laboratory Association. The survey examined laboratory approaches to evaluating bleeding disorders, with specific questions about the tests and test panels offered and compliance to recent guideline recommendations on diagnosing von Willebrand disease (VWD) and platelet function disorders. All laboratories responding to the survey performed a prothrombin time/international normalized ratio, an activated partial thromboplastin time, and coagulation factor assays, and many tested for VWD and platelet disorders. However, few laboratories had test panels that evaluated the more common bleeding disorders and few performed some assays, including VWF multimer assessments and assays for fibrinolytic disorders. Additionally, the cutoffs used by laboratories to diagnose type 1 VWD varied considerably, with only a minority following the National Heart Lung Blood Institute recommendations. In contrast, laboratories that tested for platelet function disorders mostly complied with aggregation testing recommendations, as published in the recent North American guidelines. Our results indicate that there are some gaps in the strategies used by laboratories to diagnose bleeding disorders that might be addressed by development of further guidelines and test algorithms that emphasize evaluations for common bleeding disorders. Laboratories may also benefit from guidelines on test interpretation, and external evaluation of their bleeding disorder testing strategies. Am. J. Hematol. 2012. © 2012 Wiley Periodicals, Inc.
What's NOT to eat—food adulteration in the context of human biology
Authors: Schell Lawrence M. , Gallo Mia V. , Cook Katsi 
Published: January 2012

AbstractFood has nutritional and non‐nutritional components. The latter are not well‐studied despite the fact that food adulteration has been common. Food adulteration may have reached its peak in cities of Western Europe and the US in the 18th and 19th centuries when foods were often purposely contaminated with additives to increase bulk, attractiveness, disguise spoilage, and increase profit. Effective regulation of food began in the late 19th and 20th centuries. Nevertheless, today food recalls for bacterial contamination are common, while pesticides and compounds from manufacturing are detected in many foods. Foods with strong reputations for healthiness, such as salmon, may have sizable contaminant contents. The contaminant content of many foods varies by origin and season. Nearly all commercially raised salmon has higher contaminant levels than wild caught salmon. Opting out of the commercial food distribution system is an option, but the value depends on the habitat in which the food is obtained. Traditionally, the Akwesasne Mohawk Nation has depended on local fish and wildlife for their diet. Now pollution of local waterways has led to the contamination of many local foods, and levels of the contaminant polychlorinated biphenyls in the Akwesasne Mohawk people reflect current or past dietary patterns. Many other communities in nonurban settings are exposed to contaminants through long‐trail distribution of contaminants in food, air, and/or water. Human biologists considering nutrition, disease, growth, reproduction, aging, to name a few areas, may consider the non‐nutritional components of food as many have the ability to alter physiological functioning. Am. J. Hum. Biol., 2012. © 2012 Wiley Periodicals, Inc.
Electrical Resistivity Surveying and Pseudo‐Three‐Dimensional Tomographic Imaging at Sitio Drago, Bocas del Toro, Panama
Authors: Wake Thomas A. , Mojica Alexis O. , Davis Michael H. , Campbell Christina J. , Mendizabal Tomas 
Published: January 2012

ABSTRACTResults of electrical resistivity surveying and pseudo‐three‐dimensional tomographic imaging at the 15 ha Pre‐Hispanic archaeological site of Sitio Drago, Isla Colon, Bocas del Toro province, Panama are presented. The site was occupied between ad 690–1410 and represents the largest known nucleated settlement in the province. A low mound in the centre of the site was selected for intensive electrical surveying in order to locate buried archaeological features. A series of surveys were conducted, including electrical resistivity mapping and pseudo‐three‐dimensional tomographic imaging. The results revealed a group of resistivity anomalies associated with buried archaeological features. Based on these results, archaeological excavations were targeted that uncovered a cluster of coral slab structures, each containing human remains. Copyright © 2012 John Wiley & Sons, Ltd.
Preparation and Characterization of Composites that Contain Small Carbon Nano‐Onions and Conducting Polyaniline
Authors: Plonska‐Brzezinska Marta E. , Mazurczyk Julita , Palys Barbara , Breczko Joanna , Lapinski Andrzej , Dubis Alina T. , Echegoyen Luis 
Published: January 2012

AbstractSmall multilayer fullerenes, also known as carbon nano‐onions (CNOs; 5–6 nm in diameter, 6–8 shells), show higher reactivity than other larger carbon nanostructures. Here we report the first example of an in situ polymerization of aniline on phenyleneamine‐terminated CNO surfaces. The green, protonated, conducting emeraldine polyaniline (PANI) was directly synthesized on the surface of the CNO. The functionalized and soluble CNO/PANI composites were characterized by TEM, SEM, DSC, Raman, and infrared spectroscopy. The electrochemical properties of the conducting CNO/PANI films were also investigated. In comparison with pristine CNOs, functionalized carbon nanostructures show dramatically improved solubility in protic solvents, thus enabling their easy processing for coatings, nanocomposites, and biomedical applications.
Isothiourea‐Mediated Asymmetric O‐ to C‐Carboxyl Transfer of Oxazolyl Carbonates: Structure–Selectivity Profiles and Mechanistic Studies
Authors: Joannesse Caroline , Johnston Craig P. , Morrill Louis C. , Woods Philip A. , Kieffer Madeleine , Nigst Tobias A. , Mayr Herbert , Lebl Tomas , Philp Douglas , Bragg Ryan A. , Smith Andrew D. 
Published: January 2012

AbstractThe structural motif within a series of tetrahydropyrimidine‐based isothioureas necessary for generating high asymmetric induction in the asymmetric Steglich rearrangement of oxazolyl carbonates is fully explored, with crossover and dynamic 19F NMR experiments used to develop a mechanistic understanding of this transformation.
Synthesis of Tetrasubstituted Alkenes through a Palladium‐Catalyzed Domino Carbopalladation/CH‐Activation Reaction
Authors: Tietze Lutz F. , Hungerland Tim , Düfert Alexander , Objartel Ina , Stalke Dietmar 
Published: January 2012

AbstractHelical tetrasubstituted alkenes (7) were obtained in a highly efficient way through a palladium‐catalyzed domino‐carbopalladation/CH‐activation reaction of propargylic alcohols 6 in good to excellent yields. Electron‐withdrawing‐ and electron‐donating substituents can be introduced onto the upper and lower aromatic rings. The substrates (6) for the domino process were synthesized by addition of the lithiated alkyne (20) to various aldehydes (19); moreover, the substrates were accessible enantioselectively (in 95 % ee) by reduction of the corresponding ketone using the Noyori procedure.
Reversible Solvatomagnetic Switching in a Spongelike Manganese(II)–Copper(II) 3D Open Framework with a Pillared Square/Octagonal Layer Architecture
Authors: Ferrando‐Soria Jesús , Ruiz‐García Rafael , Cano Joan , Stiriba Salah‐Eddine , Vallejo Julia , Castro Isabel , Julve Miguel , Lloret Francesc , Amorós Pedro , Pasán Jorge , Ruiz‐Pérez Catalina , Journaux Yves , Pardo Emilio 
Published: January 2012

AbstractThe concept of “molecular magnetic sponges” was introduced for the first time in 1999 by the creative imagination of the late Olivier Kahn. It refers to the exotic spongelike behavior of certain molecule‐based materials that undergo a dramatic change of their magnetic properties upon reversible dehydration/rehydration processes. Here we report a unique example of a manganese(II)–copper(II) mixed‐metal–organic framework of formula [Na(H2O)4]4[Mn4{Cu2(mpba)2(H2O)4}3]⋅ 56.5 H2O (1) (mpba=N,N′‐1,3‐phenylenebis(oxamate)). Compound 1 possesses a 3D MnII4CuII6 pillared layer structure with mixed square and octagonal pores of approximate dimensions 1.2×1.2 nm and 2.1×3.0 nm, respectively, hosting a large amount of crystallization H2O molecules and hydrated NaI countercations as guests. It reversibly switches from a crystalline hydrated phase with long‐range ferromagnetic ordering at a rather high critical temperature (Tc) of 22.5 K to an amorphous dehydrated phase with Tc as low as 2.3 K, which is accompanied by a breathing‐type dynamic effect involving a large crystal volume (ca. 45 %) and color changes after water desorption/adsorption. The combination of both the open‐framework structure and the spongelike optical, mechanical, and magnetic switching behavior in this new class of oxamato‐based porous magnets offers fascinating possibilities in designing multifunctional materials for host–guest molecular sensing.
Theoretical studies on the structure and electronic properties of cubic gold nanoclusters
Authors: Majumdar D. , Roszak Szczepan , Leszczynski Jerzy 
Published: January 2012

AbstractA multi‐scale approach involving molecular mechanics, semi‐empirical and density functional techniques have been carried out on the cubic gold nanoclusters (Aun, n = 63, 126, 252, 504, 756, 1008, 1260, 1512, 1764 and 2016) to monitor their structural and electronic properties. Definite correlations have been found to connect the ionisation potentials, conductance and absorption properties of such clusters with their shapes and sizes. Analytical equations are developed based on three‐dimensional particle in a box model to explain the validity of such correlations. © 2012 Canadian Society for Chemical Engineering
An Electropolymerized Pyrrole‐based Coating for Stir Bar Sorptive Extraction of Btex from Water Followed by Gas Chromatography‐Mass Spectrometry
Authors: Esmaeil Babanezhad , Mirabi Ali , Ghodrati Roghoieh 
Published: January 2012

AbstractA stir bar sorptive extraction (SBSE) technique was developed by the use of polypyrrole (PPy) sorbent, electropolymerized on the surface of a rod, as a possible alternative to solid‐phase microextraction (SPME). Liquid desorption was subsequently employed to transfer the extracted analytes into the injection port of a gas chromatography‐mass spectrometry (GC‐MS). The PPy sorbent including polypyrrole‐dodecyl sulfate (PPy‐DS) was deposited on the surface of a stainless steel rod from the corresponding aqueous electrolyte by applying a constant deposition potential. The developed method was applied to the trace level extraction of BTEX (benzene, toluene, ethylbenzene, o,p‐xylene) from aqueous sample. Optimization of influential experimental conditions including the voltage of power supply, the time of PPy electrodeposition, the extraction temperature, the ionic strength and the extraction time were also investigated. The detection limits of the method under optimized conditions were in the range of 0.01–0.1 ng·mL−1. The relative standard deviations (RSD) at a concentration level of 1 ng·mL−1 were obtained between 8% and 13% (n=6). The calibration curves of BTEX showed linearity in the range of 0.03 to 600 ng·mL−1. The proposed method was successfully applied to the extraction of some selected BTEX from river water samples and the relative recoveries were higher than 90% for all the analytes.
Humidity Sensing Properties of the Sensor Based on V‐doped Nanoporous Ti0.9Sn0.1O2 Thin Film
Authors: Anbia Mansoor , Moosavi Fard Seyyed Ebrahim , Shafiei Korosh , Hassanzadeh Mohammad Ali , Mayahipour Abbas 
Published: January 2012

AbstractVanadium doped nanoporous Ti0.9Sn0.1O2 thin film has been prepared on an alumina substrate by sol‐gel method with Pluronic P123 as the organic template, and humidity sensing properties of it has been investigated. It is found that V‐doped nanoporous Ti0.9Sn0.1O2 thin film shows good humidity sensing properties, and impendence of it decreases more than four orders of magnitude in the relative humidity (RH) range of 11%–95% at 25°C. The response and recovery time of this sensor are about 13 and 17 s, respectively. High sensitivity, narrow hysteresis loop, rapid response and recovery, prominent stability and good repeatability are obtained. A possible mechanism is suggested to explain the humidity sensitive properties.
Pseudopolyrotaxanes of Cucurbit[6]uril: A Three‐Dimensional Network Self‐assembled by ClO4−(H2O)2 Water Clusters
Authors: Sun Xuzhuo , Li Bo , Cao Jin , Chen Jing , Wang Ning , Wan Dongjin , Zhang Haibo , Zhou Xiaohai 
Published: January 2012

AbstractA pseudorotaxane of cucurbit[6]uril (CB[6]) with guest molecule N,N′‐hexamethylenebis (pyrazinyl perchlorate) (BPHP) was synthesized and characterized by 1H NMR spectra, IR, single crystal X‐ray diffraction analysis and thermogravimetric analysis. The structure of the pseudorotaxane (CB[6]·BPHP) is stabilized by host‐guest hydrogen bonds. Self‐assembly of the pseudorotaxane produces infinite one‐dimensional and two‐dimensional networks with intermolecular hydrogen bonds. In the molecular packing of the CB[6]·BPHP, ClO4−(H2O)2 water clusters serve as bridges to associate these pseudorotaxanes and form three‐dimensional networked pseudopolyrotaxane.
Mechanism of Silver(I)‐Catalyzed Enantioselective Synthesis of Axially Chiral Allenes Based on Propargylamines
Authors: Liao Chuanan , Li Bin , Wang Jianyi , Wang Yipin 
Published: January 2012

AbstractThe silver(I)‐catalyzed synthesis picture of axially chiral allenes based on propargylamines has been outlined using density functional theory (DFT) method for the first time. Our calculations find that, the coordination of silver(I) into triple bond of propargylamines at anti‐position of nitrogen shows a stronger activation on the triple bond than that at cis‐position, which is favorable for the subsequent hydrogen transfer. The NBO charge analysis for the hydrogen transfer affirms the experimental speculation that this step is a hydride transfer process. The energy barrier of the anti‐periplanar elimination of vinyl‐silver is 26.9 kJ·mol−1 lower than that of the syn‐periplanar elimination, supporting that (−)‐allene is the main product of this reaction. In a word, the most possible route for this reaction is that the silver(I) coordinates into the triple bond of propargylamines at anti‐position of nitrogen, then the formed silver(I) complex undergoes a hydride transfer to give a vinyl‐silver, finally the vinyl‐silver goes through an anti‐periplanar elimination to give (−)‐allene. The hydride transfer with the energy barrier of 44.8 kJ·mol−1 is the rate‐limiting step in whole catalytic process. This work provides insight into why this reaction has a very high enantioselectivity.
VEGF 165b in the developing vasculatures of the fetal human eye
Authors: Baba Takayuki , McLeod D. Scott , Edwards Malia M. , Merges Carol , Sen Tanusree , Sinha Debasish , Lutty Gerard A. 
Published: January 2012

AbstractVEGF165b is an anti‐angiogenic form of VEGF165 produced by alternative splicing. The localization of pro‐angiogenic VEGF165 and anti‐angiogenic VEGF165b was investigated during development of the vasculatures in fetal human eyes from 7 to 21 weeks gestation (WG). The fetal vasculature of vitreous, which includes tunica vasculosa lentis (TVL), had moderate VEGF165 immunoreactivity at 7WG and very little VEGF165b. Both forms were elevated at 12WG. VEGF165 then decreased around 17WG when the TVL regresses but VEGF165b remained elevated. In choroid, VEGF165 was present in forming choriocapillaris (CC) and retinal pigment epithelium (RPE) at 7WG while VEGF165b was present in CC and mesenchymal precursors within the choroidal stroma. By 21WG, both forms were elevated in RPE and choroidal blood vessels but VEGF165b was apical and VEGF165 basal in RPE. Diffuse VEGF165 immunoreactivity was prominent in 12WG innermost retina where blood vessels will form while VEGF165b was present in most CXCR4+ progenitors in the inner neuroblastic layer and migrating angioblasts in the putative nerve fiber layer. By 21WG, VEGF165 was present in nerve fibers and VEGF165b in the inner Muller cell process. The localization of VEGF165b was distinctly different from VEGF165 both spatially and temporally and it was often associated with nucleus in progenitors. Developmental Dynamics 241:595–607, 2012. © 2012 Wiley Periodicals, Inc.
Defective DNA repair and cell cycle arrest in cells expressing Merkel cell polyomavirus T antigen
Authors: Demetriou Stephanie K. , Ona‐Vu Katherine , Sullivan Erin M. , Dong Tiffany K. , Hsu Shu‐Wei , Oh Dennis H. 
Published: January 2012

AbstractThe pathways by which Merkel cell polyomavirus (MCV) infection contributes to the formation of Merkel cell carcinomas are important for understanding the pathogenesis of these cancers. We hypothesized that MCV T antigen suppresses normal responses to ultraviolet radiation (UVR)‐induced DNA damage. An MCV‐infected cell line (MKL‐1) exhibited UVR hypersensitivity, impaired repair of DNA lesions and cell cycle arrest after UVR, as well as reduced levels of the DNA damage recognition protein, XPC. When ectopically expressed in uninfected UISO cells, mutant but not wild‐type T antigen resulted in loss of repair of UVR‐induced cyclobutane pyrimidine dimers and reductions in XPC, p53 and p21 levels, whereas both wild‐type and mutant T antigen inhibited cell cycle arrest after UVR. Similarly, only mutant T antigen in normal fibroblasts inhibited DNA repair and XPC expression, while both mutant and wild‐type T antigens produced cell cycle dysregulation. Wild‐type T antigen expression produced large T, 57 kT and small T antigens while mutant T antigen was only detectable as a truncated large T antigen protein. Expression of wild‐type large T antigen but not small T antigen inhibited the G1 checkpoint in UISO cells, but neither wild‐type large T nor small T antigens affected DNA repair, suggesting that large T antigen generates cell cycle defects, and when mutated may also impair DNA repair. These results indicate that T antigen expression by MCV can inhibit key responses to UVR‐induced DNA damage and suggest that progressive MCV‐mediated abrogation of genomic stability may be involved in Merkel cell carcinogenesis.
Paracyclophanes in Action: Asymmetric Catalytic Dialkylzinc Addition to Imines Using [2.2]Paracyclophane‐based N,O‐Ligands as Catalysts
Authors: Dahmen Stefan , Bräse Stefan 
Published: January 2012

AbstractThe asymmetric addition of dibutylzinc to imines is achieved by employing [2.2]paracyclophane‐based ketimine ligands with good to excellent enantioselectivities. A comparison to other organozinc reagent is made.
Spouse‐assisted training in pain coping skills and the outcome of multidisciplinary pain management for chronic low back pain treatment: A 1‐year randomized controlled trial
Authors: Abbasi M. , Dehghani M. , Keefe F.J. , Jafari H. , Behtash H. , Shams J. 
Published: January 2012

AbstractThis study examined the comparative efficacy of three interventions: a spouse‐assisted coping skills training protocol for patients undergoing a multidisciplinary pain management programme (SA‐MPMP), conventional patient‐oriented multidisciplinary pain management programme (P‐MPMP) and standard medical care (SMC). Thirty‐six chronic low back pain (CLBP) patients and their spouses were randomly assigned to one of the three conditions. The SA‐MPMP condition consisted of seven, weekly, 2‐h, group sessions of training in dyadic pain coping and couple skills, delivered by a clinical psychologist with support of a multidisciplinary team of specialists, to patients together with their spouses. P‐MPMP consisted of the SA‐MPMP training delivered to the patient only (i.e., no spouse participation and assistance). The SMC condition entailed continuation of routine treatment, entailing medical care only. Data analysis revealed that, at the 12‐month follow‐up time point, patients receiving SA‐MPMP had significant improvements in kinesiophobia and rumination about pain compared to those receiving P‐MPMP and SMC. In patients suffering from CLBP, an intervention that combines spouse‐assisted coping skills training with a multidisciplinary pain management programme can improve fear of movement and rumination about low back pain.
Aromatic Heterocycles Used as Terminal Groups of Calamitic Liquid Crystals
Authors: Liu Chih‐Yin , Huang Chin‐Hsu , Cheng Pao‐Swu , Tsai Wen‐Liang 
Published: January 2012

AbstractThree series of compounds 1‐21 were prepared by using 2‐, 3‐, and 4‐pyridinemethanol as terminal group of calamitic liquid crystals. These compounds have some similarities in their phase behavior. They all exhibit SmA phase. A tilt smectic phase, SmC, will be formed when their flexible alkoxy chain is longer than hexoxy group. The profound difference between them is that the compounds 15‐17 possess a short temperature range of N phase. The compounds 3 and 6 have the widest SmA phase, up to 94 °C, and SmC phase, up to 46 °C, respectively.
Thiobencarb Degradation by TiO2 Photocatalysis: Parameter and Reaction Pathway Investigations
Authors: Lai Hsiao‐Fang , Chen Chiing‐Chang , Wu Ren‐Jang , Lu Chung‐Shin 
Published: January 2012

AbstractThe present study deals with the photocatalytic degradation of the thiocarbamate herbicide, thiobencarb (TBC), in the presence of TiO2 particles and UV‐A (λ = 365 nm) radiation. Results show rapid and complete oxidation of TBC after 90 min, and slightly over 70% of TBC was mineralized after 32‐h treatment. Factors such as solution pH, TiO2 dosage, and the presence of anions are found to influence the degradation rate. The establishment of the reaction pathway is made possible by a thorough analysis of the reaction mixture identifying the main intermediate products generated. Results suggest that possible transformation pathways may include hydroxylation, dealkylation and C—S bond cleavage processes. The possible degradation pathways are proposed and discussed on the basis of the evidence of oxidative intermediate formation.