bluuebunny/crossref_metadata_2025_split huggingface dataset:
Platelet‐derived growth factor‐BB induces cystathionine γ‐lyase expression in rat mesangial cells via a redox‐dependent mechanism
Authors: Hassan Mohamed I. , Boosen Meike , Schaefer Liliana , Kozlowska Jowita , Eisel Florian , von Knethen Andreas , Beck Martina , Hemeida Ramadan A. M. , El‐Moselhy Mohamed A. M. , Hamada Farid M. A. , Beck Karl‐Friedrich , Pfeilschifter Josef 
Published: March 2012

BACKGROUND AND PURPOSE So far, there is only limited information about the regulation of the endogenous synthesis of hydrogen sulfide (H2S), an important gaseous signalling molecule. This study was done to evaluate the redox‐dependent signalling events that regulate the expression of the H2S synthesising enzyme cystathionine‐γ‐lyase (CSE) in rat mesangial cells.EXPERIMENTAL APPROACH The effects of platelet‐derived growth factor (PDGF)‐BB and antioxidants on CSE expression and activity in cultured rat renal mesangial cells were assessed. Activity of nuclear factor erythroid‐2‐related factor‐2 (Nrf2) was measured as the binding capacity to a radiolabelled consensus element by electrophoretic mobility shift assay (EMSA). Furthermore, CSE and Nrf2 expression was analysed in a rat model of anti‐Thy‐1‐induced glomerulonephritis by immunohistochemistry.KEY RESULTS Treatment of mesangial cells with PDGF‐BB resulted in a marked time‐ and dose‐dependent up‐regulation of CSE mRNA and protein levels, as well as CSE activity accompanied with increased formation of reactive oxygen species. Remarkably, co‐administration of antioxidants, such as N‐acetylcysteine, ebselen or diphenylene iodonium chloride, drastically reduced PDGF‐BB‐induced CSE expression. PDGF‐BB induced binding of Nrf2 to a corresponding consensus antioxidant element in a redox‐dependent manner. Furthermore, PDGF‐BB‐induced CSE expression in mouse mesangial cells was completely abolished in Nrf2 knockout mice compared with wild‐type mice. In a rat model of anti‐Thy‐1‐induced proliferative glomerulonephritis, we observed a marked up‐regulation of CSE protein paralleled by a stabilization of Nrf2 protein.CONCLUSIONS AND IMPLICATIONS PDGF‐BB regulated CSE via a redox‐mediated activation of Nrf2. Such action would aid the resolution of glomerular inflammatory diseases.LINKED ARTICLE This article is commented on by Gallyas, pp. 2228–2230 of this issue. To view this commentary visit http://dx.doi.org/10.1111/j.1476‐5381.2012.01976.x
Effectiveness of Combining Plasma Exchange With Continuous Hemodiafiltration on Acute Fatty Liver of Pregnancy Complicated by Multiple Organ Dysfunction
Authors: Chu Yu‐Feng , Meng Mei , Zeng Juan , Zhou Hai‐Yan , Jiang Jin‐Jiao , Ren Hong‐Sheng , Zhang Ji‐Cheng , Zhu Wen‐Ying , Wang Chun‐Ting 
Published: March 2012

AbstractAcute fatty liver of pregnancy (AFLP) is a rare disease of progressive hepatic insufficiency and secondary systemic complications that induce significant maternal risk. The application of combining plasma exchange (PE) and continuous hemodiafiltration (CHDF) is a novel concept for patients with AFLP. Since 2002, we have utilized the combination of PE with CHDF as adjunctive medical therapy for 11 AFLP patients with multiple organ dysfunction. Before PE and CHDF initiation, four patients had signs and symptoms of encephalopathy, four required ventilatory support, and all 11 were developing liver failure, significant renal compromise, and coagulopathy. PE combined with CHDF for patients was initiated a mean of 2 days postpartum (range, days 0–3). Daily or every other day PE combined with CHDF was undertaken on two to eight occasions for each of the 11 patients. Ten patients responded with composite clinical and laboratory improvement and were discharged to the ward, then cured and discharged from hospital; one patient died of septic shock. Average duration of hospitalization was 17 days (range, days 9–38) from time of admission to discharge; the average duration of intensive care unit was 10 days (range, days 4–23). No significant PE‐ and CHDF‐related complications occurred. These results indicate that combing PE and CHDF in a series‐parallel circuit is an effective and safe treatment for patients with severe AFLP. This finding may have important implications for the development of an effective treatment for patients with AFLP suffering multiple organ dysfunction.
Hemodiafiltration in Infants With Complications During Peritoneal Dialysis
Authors: Rödl Siegfried , Marschitz Ingrid , Mache Christoph J. , Nagel Bert , Koestenberger Martin , Zobel Gerfried 
Published: March 2012

AbstractEnd‐stage renal disease (ESRD) in neonates still has a high mortality, particularly in the first year of life. We present the combination of peritoneal dialysis (PD) with intermittent hemodiafiltration (iHDF) in neonates with ESRD. Four infants younger than 28 days were treated with PD and iHDF. Renal diagnoses leading to ESRD were cortical necrosis, prune belly syndrome, neonatal hemolytic uremic syndrome, and autosomal recessive polycystic kidney disease. Initially, three patients were on iHDF until PD was started. At the time when complications occurred during PD, patients were switched back to iHDF. iHDF was used five times as a bridge to PD in case of abdominal surgery. Two of the four patients were switched to iHDF because of peritoneal ultrafiltration failure due to recurrent peritoneal leaks. Once, iHDF became necessary due to refractory peritonitis. All four patients survived the first year of life. Two patients were transplanted successfully at an age of 35 and 22 months, respectively. The others are on renal replacement therapy, one on PD at the age of 28 months and one on iHDF at the age of 25 months, respectively. In case of PD complications, iHDF may be an appropriate bridge to achieve long‐term survival until kidney transplantation.
Imaging language networks before and after anterior temporal lobe resection: Results of a longitudinal fMRI study
Authors: Bonelli Silvia B. , Thompson Pamela J. , Yogarajah Mahinda , Vollmar Christian , Powell Robert H. W. , Symms Mark R. , McEvoy Andrew W. , Micallef Caroline , Koepp Matthias J. , Duncan John S. 
Published: March 2012

SummaryPurpose:  Anterior temporal lobe resection (ATLR) controls seizures in up to 70% of patients with intractable temporal lobe epilepsy (TLE) but, in the language dominant hemisphere, may impair language function, particularly naming. Functional reorganization can occur within the ipsilateral and contralateral hemispheres. We investigated reorganization of language in left‐hemisphere–dominant patients before and after ATLR; whether preoperative functional magnetic resonance imaging (fMRI) predicts postoperative naming decline; and efficiency of postoperative language networks.Methods:  We studied 44 patients with TLE due to unilateral hippocampal sclerosis (24 left) on a 3T GE‐MRI scanner. All subjects performed language fMRI and neuropsychological testing preoperatively and again 4 months after left or right ATLR.Key Findings:  Postoperatively, individuals with left TLE had greater bilateral middle/inferior frontal fMRI activation and stronger functional connectivity from the left inferior/middle frontal gyri to the contralateral frontal lobe than preoperatively, and this was not observed in individuals with right TLE. Preoperatively, in left and right TLE, better naming correlated with greater preoperative left hippocampal and left frontal activation for verbal fluency (VF). In left TLE, stronger preoperative left middle frontal activation for VF was predictive of greater decline in naming after ATLR. Postoperatively, in left TLE with clinically significant naming decline, greater right middle frontal VF activation correlated with better postoperative naming. In patients without postoperative naming decline, better naming correlated with greater activation in the remaining left posterior hippocampus. In right TLE, naming ability correlated with left hippocampal and left and right frontal VF activation postoperatively.Significance:  In left TLE, early postoperative reorganization to the contralateral frontal lobe suggests multiple systems support language function. Postoperatively, ipsilateral recruitment involving the posterior hippocampal remnant is important for maintaining language, and reorganization to the contralateral hemisphere is less effective. Preoperative left middle frontal activation for VF was predictive of naming decline in left TLE after ATLR.
Early onset West syndrome with severe hypomyelination and coloboma‐like optic discs in a girl with SPTAN1 mutation
Authors: Writzl Karin , Primec Zvonka Rener , Stražišar Barbara Gnidovec , Osredkar Damjan , Pečarič‐Meglič Nuška , Kranjc Branka Stirn , Nishiyama Kiyomi , Matsumoto Naomichi , Saitsu Hirotomo 
Published: March 2012

SummaryRecent study has shown that mutations in the alpha‐II‐spectrin (SPTAN1) gene cause early onset intractable seizures, severe developmental delay, diffuse hypomyelination, and widespread brain atrophy. We report a Slovene girl with hypotonia, lack of visual attention, early onset epileptic encephalopathy, and severe developmental delay. The patient presented with segmental myoclonic jerks at the age of 6 weeks, and infantile spasms at the age of 3.5 months. Her seizures were resistant to treatment. Multiple electroencephalography recordings showed deterioration of the background activity, followed by multifocal abnormalities before progressing to hypsarrhythmia. Ophthalmologic examination revealed bilateral dysplastic, coloboma‐like optic discs. Brain magnetic resonance imaging showed diffusely reduced white matter and brainstem volumes with hypomyelination. A de novo heterozygous in‐frame deletion was detected in SPTAN1: c.6619_6621delGAG (p.E2270del). This report supports the causative relationship between SPTAN1 mutations and early onset intractable seizures with severe hypomyelination and widespread brain volume reduction. Coloboma‐like optic discs might be an additional feature observed in patients with SPTAN1 mutations.
Attitudes and Expectations Regarding Exercise in the Hospital of Hospitalized Older Adults: A Qualitative Study
Authors: So Cynthia , Pierluissi Edgar 
Published: March 2012

ObjectivesTo describe expectations of, and perceived motivators and barriers to, in‐hospital exercise of hospitalized older adults.DesignQualitative study using the framework theory.SettingPublic hospital general medical wards.ParticipantsTwenty‐eight English‐ or Spanish‐speaking inpatients aged 65 to 103.MeasurementsSemistructured interviews were conducted at the bedside. Questions explored attitudes and expectations regarding in‐hospital exercise. Interviews were tape recorded and transcribed, and content analysis was performed to identify major themes.ResultsFor most participants (71%), exercise in the hospital meant walking. Only 29% of participants expected to be exercising in the hospital, although three‐quarters perceived it to be appropriate. Major themes included motivating factors and barriers to in‐hospital exercise. Motivating factors included avoiding the negative effects of prolonged bed rest, promoting a sense of well‐being, promoting functional recovery, and being asked to exercise. Barriers included symptoms related to one's illness, institutional barriers, and fear of injury. Most respondents (85%) felt that if the physician suggested exercise, it would influence their decision to do so, yet few (27%) reported that they had spoken to their physician about exercise.ConclusionHospitalized older adults have positive perceptions about in‐hospital exercise, although they must overcome significant barriers to do so. Medical professionals have a strong influence over the exercise behavior of elderly adults in the hospital yet infrequently address the issue. Incorporating motivating factors and removing barriers may increase the effectiveness of in‐hospital exercise programs.
A Novel Approach to Facilitating Balloon Crossing Chronic Total Occlusions: The “Wire‐Cutting” Technique
Authors: HU XIN‐QUN , TANG LIANG , ZHOU SHENG‐HUA , FANG ZHEN‐FEI , SHEN XIANG‐QIAN 
Published: March 2012

Background:
Following successful guidewire passage, inability to cross the occluded lesion with a balloon is the most common cause of procedural failure for percutaneous recanalization of chronic total occlusions (CTOs). We sought to evaluate the efficacy of “wire‐cutting” technique for facilitating the balloon passage through the lesion during CTO‐percutaneous coronary intervention.
Methods:
Sixteen consecutive patients with CTOs were treated by “wire‐cutting” technique when a lubricious balloon could not be passed through the lesion after successful guidewire crossing. The main process of the “wire‐cutting” technique involved: inserting 2 percutaneous transluminal coronary angioplasty (PTCA) guidewires (A and B) into the distal true lumen; advancing a balloon over guidewire A to the site of the occlusion abutting the proximal cap, the balloon was then inflated and guidewire B was pressed between the balloon and the proximal cap; rapidly withdrawing guidewire B produced a cutting power that crushed the proximal cap thus facilitating balloon crossing. Technique success was defined as balloon having crossed CTO lesions successfully after performing wire‐cutting procedure.
Results:
Both technical success and procedural success were obtained in 10 (62.5%) cases. Technique failure occurred in 6 (37.5%) cases, of which 2 were due to the inability to place a second guidewire into the distal true lumen and the other 4 were due to severe coronary calcification. Of these 4 failures, 2 have final procedure success with the use of Rotablator and the remaining 2 patients were referred for bypass surgery. Major complication included severe coronary dissection that occurred in 1 (6.3%) patient, which was successfully treated by stenting. No other complications were observed.
Conclusion:
The wire‐cutting technique may be considered as a simple and effective approach for facilitating balloon crossing resistant CTO lesions. (J Interven Cardiol 2012;25:297–303)
We‐Talk, Communal Coping, and Cessation Success in a Couple‐Focused Intervention for Health‐Compromised Smokers
Authors: Rohrbaugh Michael J. , Shoham Varda , Skoyen Jane A. , Jensen Michaeline , Mehl Matthias R. 
Published: March 2012

摘要目的:我们考察健康受损的吸烟者及其配偶使用第一人称复数代词的情况(我们–话语),以此作为适应性共同解决问题过程的一种可能的隐形标志。方法:其中一方心脏或肺部有问题,但一方或双方依然吸烟的20对夫妇参加多达10场专为改善共同应对而设计的家庭咨询(FAMCON)戒烟治疗,在治疗中,夫妇双方把吸烟定义为’我们的’问题,而不是’你的’或’我的’问题,并采取协作行动来解决问题。我们采用自动文本分析软件《语言获得和词汇计数》(LIWC)把治疗前婚姻互动任务及干预后期的文字本中夫妇双方第一人称代词的使用情况列表。结果:在相关和回归分析中,病人配偶(而非病人本人)在治疗前的我们—话语使用情况预测了病人在戒烟12个月后是否保持戒烟;夫妇双方在干预治疗期间(控制到基线水平)我们—话语使用情况的残余改变也预测了这个结果。结论:这些结果进一步证明了配偶使用我们—话语对病人健康的预测价值,并为共同应对作为一种可能的家庭咨询(FAMCON)变化机制提供初步证明。这些发现突显了自动文本分析在夫妇治疗研究中的效用。
Reduction of Amphibious Vehicle Resistance and Bow Swamping by Fitting a Wave Cancellation Bow Plate
Authors: LATORRE ROBERT , ARANA JULIO 
Published: March 2012

AbstractAmphibious vehicles and other blunt‐shaped floating vehicles encounter the problem of a large bow wave forming at high speeds. This wave formation is accompanied by higher resistance and at a critical speed results in bow submergence or swamping. A 1/4 scale model of a tracked amphibious vehicle was tested at speeds corresponding to 3–7 km/h full scale to investigate the phenomena. The experiment showed that at speeds of 5–6 km/h, there is a significant increase in the trim by the bow. The vehicle's “breakwater” plate was able to break up this wave and avoid swamping. Comparative tests with the vehicle fitted with the wave cancellation bow plate showed that this bow plate reduced the bow wave formation and achieved a 10% reduction in the towing resistance. The wave cancellation bow plate can reduce the vehicle's power and extend its amphibious operating range or increase its amphibious speed.
Generator Life and Power Electronics
Authors: DAVEY KENT R. , JORDAN HOWARD E. , RODRIGUEZ RIGOBERTO J. , HEBNER ROBERT E. 
Published: March 2012

AbstractThe deleterious effect of full bridge rectifiers on the output of an AC generator deserves attention from the Naval community. The harmonics observed at the stator of the generator not only introduce hysteresis and eddy current losses, but voltage spikes that impact the life of the generator. The two primary factors compromising life are localized heating due to harmonics and voltage spikes that cause insulation failure through partial discharge. Induced eddy currents are proportional to frequency squared.
One‐piece zirconia oral implants: one‐year results from a prospective cohort study. 1. Single tooth replacement
Authors: Kohal Ralf‐Joachim , Knauf Marko , Larsson Birgitta , Sahlin Herman , Butz Frank 
Published: March 2012

AbstractAimTo investigate the clinical and radiographic outcome of a one‐piece zirconia oral implant for single tooth replacement after 1 year.Materials and MethodsA total of 65 patients received a one‐stage implant surgery with immediate temporization. Standardized radiographs were taken at implant insertion and after 1 year to monitor peri‐implant bone loss. A univariate analysis of the influence of different baseline parameters on marginal bone loss from implant insertion to 12 months was performed. Soft tissue parameters were evaluated at prosthesis insertion and at the 1‐year follow‐up.ResultsAfter 1 year, three implants were lost, giving a cumulative survival rate of 95.4%. The marginal bone loss after 1 year was 1.31 mm. Thirty‐four per cent of the implants lost at least 2 mm bone, and 14% more than 3 mm. The univariate analysis could not depict any parameter influencing marginal bone loss. Probing depth, Clinical Attachment Level, Bleeding and Plaque Index decreased over 1 year.ConclusionsThe cumulative survival rate of the presented ceramic implant was comparable to the reported survival rate of titanium implants when immediately restored. However, the frequency of increased radiographic bone loss (>2 mm) after 1 year was considerably higher as compared to conventional two‐piece titanium implants. The presented zirconia implant can therefore not be recommended for clinical usage.
Effect of osteoporosis on periodontal therapy among post‐menopausal women
Authors: Gomes‐Filho Isaac S. , Oliveira Tiago J. S. , Passos Johelle S. , Cerqueira Eneida de M. M. , da Cruz Simone S. , Barreto Maurício L. , Coelho Julita M. F. , Trindade Soraya C. , Santos Carlos A. S. T. , Sarmento Viviane A. 
Published: March 2012

Objective:  This intervention study aimed to investigate the effect of osteoporosis on periodontal condition among 48 post‐menopausal women undergoing periodontal therapy.Material and methods:  The experimental group, which underwent non‐surgical periodontal therapy, was composed of 16 women with periodontitis to be treated, and the control group was formed by 32 women without periodontitis. Oral condition was assessed on three occasions: at the start of the treatment (first examination), 1 month (first re‐examination) and 4 months after the end of the therapy (second re‐examination). In the second re‐examination, recurrence of periodontal disease was evaluated by comparing the clinical measurements obtained pre‐ and post‐treatment. The diagnosis of osteoporosis was made by investigating densitometry reports obtained previously. Descriptive analysis, analysis of variance and the Bonferroni post hoc test were applied to the data gathered, with statistical significance level of 5%.Results:  The frequency of periodontitis was 50% in the treated group and 25% in the group without periodontitis. In both groups, this recurrence was greater in subjects with osteoporosis (37.5 and 18.75%, respectively) than in the individuals without osteoporosis (12.5 and 6.25%, respectively).Conclusions:  The preliminary results indicate that osteoporosis possibly has an influence on periodontal condition among individuals undergoing non‐surgical periodontal treatment.
Poly(ADP-Ribose) Polymerase Inhibition Improves Erectile Function by Activation of Nitric Oxide/Cyclic Guanosine Monophosphate Pathway in Diabetic Rats
Authors: Li Wen Ji , Peng YuBing , Zhou Juan , Li Bao , Wang Hao , Zhang Jun , Wang Zhong 
Published: March 2012

ABSTRACT

Introduction
Endothelial dysfunction-induced abnormalities of the nitric oxide (NO)/cyclic guanosine monophosphate (cGMP) signaling pathway in the corpus cavernosum are thought to be the main factors involved in the pathogenesis of diabetes-induced erectile dysfunction (ED). Recent studies have shown that the poly(adenosine diphosphate ribose) polymerase (PARP) pathway plays a critical role in diabetic endothelial dysfunction.

Aim
The aim of this study is to determine whether activation of the PARP pathway is involved in diabetic cavernosal endothelial dysfunction and abnormalities of the NO/cGMP pathway.

Methods
Male Sprague-Dawley rats were randomly divided into three groups: age-matched controls, diabetic controls (DM), and the 3-aminobenzamide (3-AB, a PARP inhibitor)-treated diabetic group (DM+3-AB). Diabetes was induced by intraperitoneal injection of streptozotocin. Eight weeks after inducing diabetes, the DM+3-AB group was treated with 3-AB for 4 weeks.

Main Outcome Measures
Erectile function was assessed at 12 weeks after inducing diabetes by stimulating the cavernous nerve. Expression of poly(ADP-ribose), protein kinase B (Akt), phospho-Akt, endothelial nitric oxide synthase (eNOS), phospho-eNOS, and neuronal nitric oxide synthase (nNOS) were evaluated by Western blot. Cavernous NO generation and cGMP levels were also determined.

Results
The DM group showed impaired erectile function and significantly increased PARP activity. Expression of total eNOS and nNOS, phospho-Akt, and eNOS decreased significantly in the DM group compared with those in the control group. In addition, cavernous NO generation and cGMP levels decreased significantly in the DM group compared with those in the control group. Treatment with 3-AB restored erectile function and significantly reversed all molecular alterations except decreased nNOS expression.

Conclusion
Overactivation of the PARP pathway in the corpus cavernosum of diabetic rats was involved in cavernosal endothelial dysfunction and abnormalities of the NO/cGMP pathway resulting in ED. These findings may be applied to develop novel therapies for patients with diabetic ED.