bluuebunny/crossref_metadata_2025_split huggingface dataset:
Adherence to practice guidelines for atypical glandular cells on cervical cytology
Authors: Sharpless Kathryn E. , King Cara R. , Schnatz Peter F. 
Published: June 2012

AbstractBACKGROUND:Atypical glandular cells (AGC) on cervical cytology are high–risk, requiring an extensive evaluation. Compliance with practice guidelines for AGC, however, has been low. Some AGC cytology reports contain cytopathologist recommendations for evaluation. This study determines whether evaluation rates for AGC have improved over time, and whether cytopathologists' recommendations correlate with the types of evaluation women receive.METHODS:Evaluation rates from 284 women with AGC (2004‐2007) were compared with findings from 1998‐2001. Rates of evaluations were compared based on cytology report recommendations.RESULTS:A total of 76.1% of the AGC cases had histologic sampling, and 58.8% had a comprehensive evaluation. These rates are higher than those from 1998‐2001 (63.5% and 35.8%, respectively; P<.01). Rates of evaluations of women with AGC “favor neoplasia” did not increase between the 2 time periods. Between 2004‐2005 and 2006‐2007, rates of comprehensive initial evaluations and endometrial sampling in women ≥35 years of age did not increase. Of the AGC reports that did contain cytopathologist recommendations, 28% were consistent with practice guidelines, 26% recommended an incomplete histologic evaluation, and 46% recommended repeat cytology. Women whose AGC report recommended a comprehensive evaluation or any histologic evaluation were more likely to have a comprehensive work–up (79%) than those whose reports did not contain recommendations (55%, P <0.01) or recommended repeat cytology (51%, P<0.02).CONCLUSIONS:Adherence to practice guidelines for the evaluation of women with AGC has improved but continues to be suboptimal. Our findings suggest that continuing education and including practice guidelines on AGC cytology reports may improve compliance. Cancer (Cancer Cytopathol) 2013;121:47–53 © 2012 American Cancer Society.
Disordered eating following exposure to television and internet coverage of the March 2011 japan earthquake
Authors: Rodgers Rachel F. , Franko Debra L. , Brunet Alain , Herbert Christophe F. , Bui Eric 
Published: June 2012

AbstractObjective:To prospectively explore the relationship between exposure to potentially distressing news content and disordered eating.Method:Within 2 weeks of the March 3, 2011 Japan earthquake, an online survey was conducted among non‐Japanese adults in distant countries (N = 698) assessing time spent on TV and the internet watching the news as well as peritraumatic reactions to the news and sleep disturbance. Participants were invited to complete a followup survey two months later [n = 113, mean age (SD) = 38.8 (11.91), 73% female] reporting on eating disorder symptoms.Results:Exposure to TV and, to a lesser extent, internet coverage of the Japan disaster were associated with disordered eating, in particular dieting and oral control, as measured by the eating attitudes test. Peritraumatic reactions and sleep disturbance displayed specific patterns of association with disordered eating.Discussion:Exposure to media coverage of distant disasters may be associated with increased disordered eating. © 2012 by Wiley Periodicals, Inc. (Int J Eat Disord 2012;45:845–849)
The role of CXCR3 and CXCR4 in colorectal cancer metastasis
Authors: Murakami Teppei , Kawada Kenji , Iwamoto Masayoshi , Akagami Masatoshi , Hida Koya , Nakanishi Yuki , Kanda Keitaro , Kawada Mayumi , Seno Hiroshi , Taketo Makoto Mark , Sakai Yoshiharu 
Published: June 2012

AbstractChemokines and their receptors play key roles in leukocyte trafficking and are also implicated in cancer metastasis. We previously demonstrated that forced expression of CXCR3 promotes colon cancer metastasis preferentially to the draining lymph nodes (LNs), with poor prognosis. Using clinical colorectal cancer (CRC) samples, here, we show that expressions of CXCR3 and CXCR4 are significantly higher in metastatic foci within LNs and liver compared to primary tumors, whereas ligands for CXCR3 and CXCR4 are not. We also have demonstrated that some human CRC cell lines constitutively express both CXCR3 and CXCR4, and that activation of CXCR3 strengthens the CXCR4‐mediated cell migration in vitro in a synergistic manner. By constructing SW620 cell lines with reduced expression of CXCR3 and/or CXCR4 using microRNA, we investigated in vivo metastatic activities in a mouse rectal transplantation model. Six weeks after inoculation, CXCR3‐, CXCR4‐, and CXCR3/CXCR4 double‐knockdowns significantly reduced metastasis to LNs, liver and lungs, compared to the control (p < 0.05). Importantly, its suppressive effect on LN metastasis was significantly stronger in CXCR3‐ and CXCR3/CXCR4 double‐knockdowns. In addition, CXCR3‐ and CXCR3/CXCR4 double‐knockdowns significantly decreased the dissemination of cancer cells to liver and lungs, even after 2 weeks. These results indicate that targeting CXCR3 and CXCR4 can be a promising therapy against CRC metastasis.
Fibrin chain cross‐linking, fibrinolysis, and in vivo sealing efficacy of differently structured fibrin sealants
Authors: Hedrich Hans Christian , Simunek Manuela , Reisinger Sonja , Ferguson James , Gulle Heinz , Goppelt Andreas , Redl Heinz 
Published: June 2012

AbstractIn this study, we compared the sealing characteristics and efficacy of a fibrin sealant with reduced plasminogen (FS‐rplg) and a fibrin sealant with aprotinin as a fibrinolysis inhibitor (FS‐apr). The relevant sealing characteristics including clot structure, fibrin chain cross‐linking, and clot lysis were tested in the laboratory. The sealing efficacy was then investigated in a follow‐up animal model to determine differences in the in vivo sealing properties. A total of 46 animals were available for the final analysis with 23 animals in each treatment arm. In conclusion, we saw differences in vitro between FS‐rplg and FS‐apr in ultrastructure and α‐chain cross‐linking rates as well as in the rate of fibrinolysis. These differences may explain the significantly enhanced sealing efficacy in FS‐apr compared to FS‐rplg shown in vivo in a rabbit intestinal model. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 100B: 1507–1512, 2012.
Synthesis and characterization of Mg‐Ca‐Sr alloys for biodegradable orthopedic implant applications
Authors: Berglund Ida S. , Brar Harpreet S. , Dolgova Natalia , Acharya Abhinav P. , Keselowsky Benjamin G. , Sarntinoranont Malisa , Manuel Michele V. 
Published: June 2012

AbstractMagnesium has recently received an increased amount of interest due to its potential use in biodegradable implant applications. The rapid degradation of conventional Mg is, however, a major limitation that needs to be addressed in the design of these materials, along with consideration of toxicity in selection of alloying elements. In this study, five alloys in the Mg‐xCa‐ySr system (x= 0.5–7.0 wt %;y= 0.5–3.5 wt %) were prepared and characterized for their suitability as degradable orthopedic implant materials. The alloys were characterized using optical microscopy, scanning electron microscopy, energy‐dispersive X‐ray spectroscopy, X‐ray diffraction, degradation measurements in Hanks' solution at 37°C, compression testing, andin vitrocytotoxicity testing with a mouse osteoblastic cell line. The results indicate that the Mg‐1.0Ca‐0.5Sr alloy is the most promising alloy for orthopedic implant applications since it showed the lowest degradation rate in Hanks' solution (0.01 mL cm−2h−1) along with no significant toxicity to MC3T3‐E1 osteoblasts and a compressive strength of 274 ± 4 MPa. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 100B: 1524–1534, 2012.
Microstructure and biological properties of micro‐arc oxidation coatings on ZK60 magnesium alloy
Authors: Pan Y. K. , Chen C. Z. , Wang D. G. , Yu X. 
Published: June 2012

AbstractCeramic coatings were prepared on ZK60 magnesium alloy in electrolyte with different concentration ratio of calcium and phosphorus (Ca/P) by micro‐arc oxidation (MAO) technique at constant voltage. The microstructure, phase composition, elemental distribution, corrosion resistance, and adhesion of the coatings were investigated by scanning electron microscope (SEM), X‐ray diffractometer (XRD), energy‐dispersive X‐ray spectrometry (EDS), electrochemical workstation, and scratch spectrometer, respectively. The coating biocompatibility was evaluated by in vitro cytotoxicity tests and systemic toxicity tests, and the bioactivity and degradability were evaluated by simulation body fluid (SBF) immersion tests. SEM shows that pores with different shapes distribute all over the coating surface. The adhesion and thickness of the coatings increases with increasing Ca/P ratio of electrolyte. The in vitro cytotoxicity tests and systemic toxicity texts demonstrate that the coatings have no toxicity to cell and living animal, which show that the coatings have excellent biocompatibility. XRD analysis shows that bioactive calciumphosphate (CaP) phases such as hydroxyapatite (HA, Ca10(PO4)6(OH)2) and calcium pyrophosphate (CPP, Ca2P2O7) are induced in the immersed coatings, indicating that the MAO coatings have excellent bioactivity. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 100B: 1574–1586, 2012.
Vitamin K, vertebral fractures, vascular calcifications, and mortality: VItamin K Italian (VIKI) dialysis study
Authors: Fusaro Maria , Noale Marianna , Viola Valentina , Galli Francesco , Tripepi Giovanni , Vajente Nicola , Plebani Mario , Zaninotto Martina , Guglielmi Giuseppe , Miotto Diego , Dalle Carbonare Luca , D'Angelo Angela , Naso Agostino , Grimaldi Cristina , Miozzo Davide , Giannini Sandro , Gallieni Maurizio ,   for the VItamin K Italian (VIKI) Dialysis Study Investigators
Published: June 2012

Abstract
Vitamin K (vitamin K1 or phylloquinone and vitamin K2, a series of menaquinones [MKs]) is involved in the production of bone and matrix amino acid γ-carboxy-glutamic acid (Gla) proteins, regulating bone and vascular calcification. Low vitamin K concentrations are associated with increased risks of fractures and vascular calcification, and frequent complications in hemodialysis patients. We carried out an observational study to establish the prevalence of vitamin K deficiency and to assess the relationship between vitamin K status, vertebral fractures, vascular calcification, and survival in 387 patients on hemodialysis for ≥1 year. We determined plasma levels of vitamin K compound, bone-Gla-protein, matrix-Gla-protein, and routine biochemistry. Vertebral fractures (reduction in vertebral body height by ≥20%) and aortic and iliac calcifications were also investigated in a spine (D5–L4) radiograph. Three-year patient survival was analyzed. Important proportions of patients had deficiency of MK7 (35.4%), vitamin K1 (23.5%), and MK4 (14.5%). A total of 55.3% of patients had vertebral fractures, 80.6% had abdominal aorta calcification, and 56.1% had iliac calcification. Vitamin K1 deficiency was the strongest predictor of vertebral fractures (odds ratio [OR], 2.94; 95% confidence interval [CI], 1.38–6.26). MK4 deficiency was a predictor of aortic calcification (OR, 2.82; 95% CI, 1.14–7.01), whereas MK5 deficiency actually protected against it (OR, 0.38; 95% CI, 0.15–0.95). MK7 deficiency was a predictor of iliac calcification (OR, 1.64; 95% CI, 1.03–2.60). The presence of vertebral fractures was also a predictor of vascular calcifications (OR, 1.76; 95% CI, 1.00–3.08). Increased alkaline phosphatase and C reactive protein (CRP), age, and cerebrovascular events were predictors of mortality. Our study suggests that the vitamin K system may be important for preserving bone mass and avoiding vascular calcification in hemodialysis patients, pointing out a possible role of vitamin K in bone and vascular health. Based on our results, we suggest that the general population should also be studied for vitamin K deficiency as a possible cause of both vertebral fractures and vascular calcification. © 2012 American Society for Bone and Mineral Research.
Risk factors for fractures and avascular osteonecrosis in type 1 Gaucher disease: A study from the International Collaborative Gaucher Group (ICGG) Gaucher Registry
Authors: Khan Aneal , Hangartner Thomas , Weinreb Neal J , Taylor John S , Mistry Pramod K 
Published: June 2012

Abstract
We hypothesized that overall disease activity or the severity of involvement of individual disease compartments, as measured by clinical and surrogate markers, predict the risk of avascular osteonecrosis (AVN) or fractures in type 1 Gaucher disease (GD1). We applied our risk-set matched case-control method to identify four patient groups within the International Collaborative Gaucher Group (ICGG) Gaucher Registry based on the presence and absence of AVN and fractures. Characteristics of GD1 were examined by comparing the distributions of each risk factor in cases versus matched controls using conditional logistic regression to calculate adjusted odds ratios (OR). Potential risk factors included hematological and visceral parameters, GD1 biomarkers, white blood cells, GBA1 genotype, and spine and femur dual-energy X-ray absorptiometry (DXA) Z-scores. In the total population of 5894 ICGG Gaucher Registry patients, 544 experienced at least one episode of AVN; 2008 reported no history of AVN. Clinical and surrogate markers of disease activity were similar in patients with and without AVN; patients with AVN were 1.6 times more likely to be anemic compared to matched controls (OR = 1.59; 95% confidence interval [CI], 1.06–2.38, p &lt; 0.05). For fractures, 319 patients suffered fractures and 1233 had no prior history of fractures. Clinical and surrogate markers of disease in patients with and without fractures were similar, except for mean lumbar spine DXA Z-scores. Among patients with fractures, 49.3% had DXA Z-scores ≤ −1 compared to 31.0% in the control group. Compared to controls with Z-scores &gt; −1.0, GD1 patients exhibiting Z-scores ≤ −1 had an OR of 5.55 (95% CI, 1.81–17.02, p &lt; 0.01) for fracture. In GD1, after controlling for gender, year of birth, treatment status, and splenectomy status, we identified new risk factors for AVN and fractures. Concurrent anemia was associated with an increased risk for AVN. Low bone mineral density of the lumbar spine was a strong risk factor for fractures of the spine and femur in GD1. © 2012 American Society for Bone and Mineral Research.
A controlled register-based study of 460 neurofibromatosis 1 patients: Increased fracture risk in children and adults over 41 years of age
Authors: Heervä Eetu , Koffert Anna , Jokinen Elina , Kuorilehto Tommi , Peltonen Sirkku , Aro Hannu T , Peltonen Juha 
Published: June 2012

Abstract
Neurofibromatosis 1 (NF1, von Recklinghausen's disease) is an autosomal dominant neurocutaneous-skeletal syndrome in which low bone mineral density (BMD) and osteoporosis are common. Low BMD is, however, not the sole component of fracture risk. In the current study, 460 Finnish patients with NF1 were identified from the hospital medical records and their fracture risk was evaluated. The control population included 3988 appendectomy patients whose age and gender distribution was similar to that of the NF1 patients. Medical records of NF1 and control cohorts were screened for fractures according to the International Statistical Classification of Diseases and Related Health Problems, 10th Revision (ICD-10) between January 2000 and October 2011. The results show that patients with NF1 had increased age-dependent fracture risk compared to controls. Specifically, patients with NF1 aged 41 years and older had a risk ratio of ×5.2 for fractures compared to controls, and children with NF1 had a ×3.4 risk ratio for fractures compared to children without NF1. In contrast, the fracture risk was not increased in NF1 patients aged 17 to 40 years. When fractures not traditionally related to osteoporosis such as fractures of fingers, toes, and skull were excluded, the results were essentially the same. No gender related differences were observed. In conclusion, patients with NF1 have increased fracture risk depending on age. We recommend considering prophylactic measures, such as lifestyle advice, to prevent fractures from occurring. © 2012 American Society for Bone and Mineral Research.
Removal of iopromide from an aqueous solution using dielectric barrier discharge
Authors: Liu Yanan , Sun Yu , Hu Jinlong , He Jun , Mei Shufang , Xue Gang , Ognier Stéphanie 
Published: June 2012

AbstractBACKGROUND: Both ionic and nonionic ICM are recalcitrant to ozone and traditional waste water treatment plants. In this study, the efficiency of one kind of ICM‐iopromide (IOPr) removal from an aqueous solution using a dielectric barrier discharge (DBD) method was investigated.RESULTS: An energy density of 1.5E05 J L−1 resulted in the most significant removal (98.8%) of IOPr. At this energy density, no decrease in total organic carbon (TOC) was observed. Based on the IR spectra, degradation of the IOPr molecule involved hydroxylation, carbonylation and deiodination. BOD5/COD measurements indicated that the biodegradability of IOPr increased significantly as a result of DBD treatment. The byproducts of IOPr after DBD treatment were more polar and easily adsorbed and biodegraded by the activated sludge. The removal of IOPr from the solution followed first‐order kinetics, with Ks of 0.10 min−1, 0.11 min−1, 0.44 min−1 and 0.15 min−1 corresponding to energy densities of 1E + 05 J L−1, 1.3E + 05 J L−1, 1.5E + 05 J L−1 and 1.8E + 05 J L−1, respectively. The kinetics of the deiodination reactions were more complex due to subsequent iodide oxidation.CONCLUSION: DBD is very effective as a pretreatment or advanced treatment method for increasing the recalcitrant chemical's biodegradability and making subsequent biological treatment more efficient. © 2012 Society of Chemical Industry
Characterisation of the volatile profile of coconut water from five varieties using an optimised HS‐SPME‐GC analysis
Authors: Prades Alexia , Assa Rebecca Rachel Ablan , Dornier Manuel , Pain Jean‐Pierre , Boulanger Renaud 
Published: June 2012

AbstractBACKGROUND: Coconut (Cocos nucifera L.) water is a refreshing tropical drink whose international market has recently been growing. However, little is yet known about its physicochemical composition, particularly its aroma. This study set out to characterise the volatile profile of water from five coconut varieties.RESULTS: Aroma compounds were characterised by headspace solid phase microextraction gas chromatography (HS‐SPME‐GC) analysis. An experimental design was established to optimise SPME conditions, leading to an equilibration time of 10 min followed by an extraction time of 60 min at 50 °C. Accordingly, immature coconut water from WAT (West African Tall), PB121 (MYD × WAT Hybrid), MYD (Malayan Yellow Dwarf), EGD (Equatorial Guinea Green Dwarf) and THD (Thailand Aromatic Green Dwarf) palms was analysed and described. Ketones were mainly present in the Tall and Hybrid varieties, whereas aldehydes were most abundant in the Dwarf palms. Tall coconut water was characterised by a high lactone content. THD exhibited a high ethyl octanoate level. The cluster analysis of the volatile fraction from the five coconut cultivars was found to be related to their genetic classification.CONCLUSION: The volatile compounds of immature coconut water from five varieties were characterised for the first time. Volatile profile analysis could be a useful tool for the selection of Dwarf coconut varieties, which are mainly consumed as a beverage. Copyright © 2012 Society of Chemical Industry
Effects of hop varieties on ruminal fermentation and bacterial community in an artificial rumen (rusitec)
Authors: Narvaez Nelmy , Wang Yuxi , Xu Zhonjou , Alexander Trevor , Garden Scott , McAllister Tim 
Published: June 2012

AbstractBACKGROUND: There is a growing interest in the use of hops (Humulus lupulus) as an alternative to antibiotics to manipulate ruminal fermentation. However, the effects of different hop varieties on ruminal fermentation and bacterial populations have not been studied. Here the effects of three hop varieties, Cascade (CAS), Millennium (MIL) and Teamaker (TM), at a level of 800 µg mL−1 inoculum on ruminal fermentation and microbial populations in an artificial rumen system (rusitec) fed a barley silage‐based total mixed ration were investigated. Bacterial populations were assessed using real‐time polymerase chain reaction and expressed as a percentage of total bacterial 16S rRNA gene copies.RESULTS: All hops reduced (P < 0.001) total gas, methane and the acetate:propionate ratio. Liquid‐associated Fibrobacter succinogenes, Ruminococcus albus and Streptococcus bovis were reduced (P < 0.05) by MIL and TM. Feed particle‐associated S. bovis was reduced (P < 0.01) by MIL and TM, but TM and CAS increased (P < 0.01) Ruminobacter amylophilus and Prevotella bryantii respectively. Methanogens were decreased (P < 0.05) by MIL in both liquid and solid fractions and by CAS in the solid fraction. The total amount of α‐ and β‐acids in hops affected the ruminal fermentation.CONCLUSION: Hop‐induced changes in fermentation and microbial populations may improve energy efficiency use in the rumen. Further research is needed to determine the effects of hops on in vivo ruminal fermentation, microbial populations and animal performance. Copyright © 2012 Society of Chemical Industry
Hydrodistillation and in situ microwave‐generated hydrodistillation of fresh and dried mint leaves: a comparison study
Authors: Orio Laura , Cravotto Giancarlo , Binello Arianna , Pignata Giuseppe , Nicola Silvana , Chemat Farid 
Published: June 2012

AbstractBACKGROUND: Hydrodistillation (HD) has been used since ancient times for the extraction of essential oils (EO). Despite the intrinsic limitations of this technique, it remains the most common method both in the laboratory and on an industrial scale. The main drawbacks are the long extraction time involved and the risk of thermal degradation. Over the last decade, microwave‐assisted extraction (MAE) and in situ microwave‐generated hydrodistillation (MGH) have been shown to be the most promising techniques in improving plant extraction and hydrodistillation.RESULTS: In this study we compare HD with MGH in the extraction of several mint species cultivated in Piedmont: Mentha spicata L. var. rubra, Mentha spicata L. var. viridis and Mentha piperita L. MGH requires either fresh plant or rehydrated material, it is extremely fast and allows a reduction in energy consumption and overall cost. All the EO have been analyzed by gas chromatography‐mass spectrometry. A mechanism of microwave‐generated essential oil extraction has been proposed to explain the differences in the composition of the oil obtained from this environmentally friendly technique.CONCLUSIONS: The yields and composition percentages of the EO obtained by HD and in situ MGH of fresh and dried mint leaves lie in a relatively narrow range, although MGH is faster. MW polarization effects and the water solubility of the components influence extract composition Copyright © 2012 Society of Chemical Industry