bluuebunny/crossref_metadata_2025_split huggingface dataset:
MmpL3, Wag31 and PlrA are involved in coordinating polar growth with peptidoglycan metabolism and nutrient availability
Authors: Arejan Neda Habibi , Czapski Desiree R. , Buonomo Joseph A. , Boutte Cara C. 
Published: April 2024

AbstractCell growth in mycobacteria involves cell wall expansion that is restricted to the cell poles. The DivIVA homolog Wag31 is required for this process, but the molecular mechanism and protein partners of Wag31 have not been described. In this study ofMycobacterium smegmatis, we identify a connection betweenwag31and trehalose monomycolate (TMM) transportermmpl3in a suppressor screen, and show that Wag31 and polar regulator PlrA are required for MmpL3’s polar localization. In addition, the localization of PlrA and MmpL3 are responsive to nutrient and energy deprivation and inhibition of peptidoglycan metabolism. We show that inhibition of MmpL3 causes delocalized cell wall metabolism, but does not delocalize MmpL3 itself. We found that cells with an MmpL3 C-terminal truncation, which is defective for localization, have only minor defects in polar growth, but are impaired in their ability to downregulate cell wall metabolism under stress. Our work suggests that, in addition to its established function in TMM transport, MmpL3 has a second function in regulating global cell wall metabolism in response to stress. Our data are consistent with a model in which the presence of TMMs in the periplasm stimulates polar elongation, and in which the connection between Wag31, PlrA and the C-terminus of MmpL3 is involved in detecting and responding to stress in order to coordinate synthesis of the different layers of the mycobacterial cell wall in changing conditions.
Inositol pyrophosphate catabolism by three families of phosphatases controls plant growth and development
Authors: Laurent Florian , Bartsch Simon M. , Shukla Anuj , Rico-Resendiz Felix , Couto Daniel , Fuchs Christelle , Nicolet Joël , Loubéry Sylvain , Jessen Henning J. , Fiedler Dorothea , Hothorn Michael 
Published: April 2024

AbstractInositol pyrophosphates (PP-InsPs) are nutrient messengers whose cellular concentration must be tightly regulated. Diphosphoinositol pentakisphosphate kinases (PPIP5Ks) generate the active signaling molecule 1,5-InsP8. PPIP5Ks contain additional phosphatase domains involved in PP-InsP catabolism. Plant and Fungi Atypical Dual Specificity Phosphatases (PFA-DSPs) and NUDIX phosphatases (NUDTs) also hydrolyze PP-InsPs. Here we dissect the relative contributions of the three different phosphatase families to plant PP-InsP catabolism and nutrient signaling. We report the biochemical characterization of inositol pyrophosphate phosphatases from Arabidopsis andMarchantia polymorpha. Overexpression of different PFA-DSP and NUDT enzymes affects PP-InsP levels and leads to stunted growth phenotypes in Arabidopsis.nudt17/18/21knock-out mutants have altered PP-InsP pools and gene expression patterns, but no apparent growth defects. In contrast,Marchantia polymorphaMppfa-dsp1ge,Mpnudt1geand Mpvip1gemutants display severe growth and developmental phenotypes associated with changes in cellular PP-InsP levels. Analysis of Mppfa-dsp1geand Mpvip1gesupports a role for PP-InsPs in Marchantia phosphate signaling, and additional functions in nitrate homeostasis and cell wall biogenesis. Simultaneous removal of two phosphatase activities enhances the observed growth phenotypes. Taken together, PPIP5K, PFA-DSP and NUDT inositol pyrophosphate phosphatases play important roles in growth and development by collectively shaping plant PP-InsP pools.Author summaryOrganisms must maintain adequate levels of nutrients in their cells and tissues. One such nutrient is phosphorus, an essential building block of cell membranes, nucleic acids and energy metabolites. Plants take up phosphorus in the form of inorganic phosphate and require sufficient cellular phosphate levels to support their growth and development. It has been shown that plants and other eukaryotic organisms "measure" cellular phosphate levels using inositol pyrophosphate signaling molecules. The concentration of inositol pyrophosphates serves as a proxy for the cellular concentration of inorganic phosphate, and therefore inositol pyrophosphate synthesis and degradation must be tightly regulated. Here, we report that three different families of enzymes contribute to the degradation of inositol pyrophosphates in plants. The different phosphatases together shape cellular inositol pyrophosphate pools and thereby affect inorganic phosphate levels. Loss-of-function mutants of the different enzymes display additional defects in nitrate levels and cell wall architecture, suggesting that inositol pyrophosphates regulate cellular processes beyond inorganic phosphate homeostasis.
Sustained innate interferon is an essential inducer of tertiary lymphoid structures
Authors: Calvanese Anna Laura , Cecconi Virginia , Stäheli Severin , Schnepf Daniel , Pereira Paulo , Gschwend Julia , Heikenwälder Mathias , Schneider Christoph , Ludewig Burkhard , Silina Karina , van den Broek Maries 
Published: April 2024

AbstractTertiary lymphoid structures (TLS) resemble follicles of secondary lymphoid organs and develop in non-lymphoid tissues during inflammation and cancer. Which cell types and signals drive the development of TLS is largely unknown.To investigate early events of TLS development in the lungs, we repeatedly instilled p(I:C) plus ovalbumin (Ova) intranasally. This induced TLS ranging from lymphocytic aggregates to organized and functional structures containing germinal centers. We found that TLS development is independent of FAP+fibroblasts, alveolar macrophages or CCL19 but crucially depends on type I (IFN-I)-but not type III interferon (IFN-III)-signaling.Mechanistically, IFN-I initiates two synergistic pathways that culminate in the development of TLS. On the one hand, IFN-I induces lymphotoxin (LT)α in lymphoid cells, which stimulate stromal cells to produce the B-cell-attracting chemokine CXCL13 through LTβR-signaling. On the other hand, IFN-I is sensed by stromal cells that produce the T-cell-attracting chemokines CXCL9, CXCL10 as well as CCL19 and CCL21 independently of LTβR. Consequently, B-cell aggregates develop within a week, whereas follicular dendritic cells and germinal centers appear after 3 weeks.Thus, sustained production of IFN-I together with an antigen is essential for the induction of functional TLS in the lungs.Graphical abstract
MeV gamma rays from
Q
-ball decay
Authors: Kasuya Shinta , Kawasaki Masahiro , Tsuji Naomi 
Published: April 2024

We study the supersymmetric Q balls which decay at present and find that they create a distinctive spectrum of gamma rays at around O(10) MeV. The charge of the Q ball is lepton numbers in order for the lifetime to be as long as the present age of the Universe, and the main decay products are light leptons. However, as the charge of the Q ball decreases, the decay channel into pions becomes kinematically allowed towards the end of the decay, and the pions are produced at rest. Immediately, π0 decays into two photons with the energy of 67.5 MeV, half the pion mass, which exhibits a unique emission line. In addition, π± decay into μ±, which further decay with emitting internal bremsstrahlung, whose spectrum has a sharp cutoff at ∼50  MeV. If the observations find these peculiar features of the gamma-ray spectrum in the future, it could be a smoking gun of the supersymmetric Q-ball decay at present.

Published by the American Physical Society
2024
Investigation on the flow and induced-noise mechanism during the transient start-up process of a mixed-flow pump
Authors: Zhang Jinsong , Wang Xinlong , Yang Chen , Sun Mingkang , Huang Zhenwei 
Published: April 2024

PurposeThis study aims to investigate the noise-inducing characteristics during the start-up process of a mixed-flow pump and the impact of different start-up schemes on pump noise.Design/methodology/approachThis study conducted numerical simulations on the mixed-flow pump under different start-up schemes and investigated the flow characteristics and noise distribution under these schemes.FindingsThe results reveal that the dipole noise is mainly caused by pressure fluctuations, while the quadrupole noise is mainly generated by the generation, development and breakdown of vortices. Additionally, the noise evolution characteristics during the start-up process of the mixed-flow pump can be divided into the initial stage, stable growth stage, impulse stage and stable operation stage.Originality/valueThe findings of this study can provide a theoretical basis for the selection of start-up schemes for mixed-flow pumps, reducing flow noise and improving the operational stability of mixed-flow pumps.
Technology meets clinical practice: Keel Mind as a digital therapy platform
Authors: Austin Sydney , Bandealy Ahad , Cawley Elizabeth 
Published: April 2024

Purpose
The purpose of this paper is to highlight current utilizations of advanced technology within the digital mental health platform Keel Mind.

Design/methodology/approach
Keel Mind, based out of Ontario, Canada is a therapeutic delivery platform that dually functions to train clinicians and deliver virtual therapy to clients.

Findings
From personalizing client and therapist matching to using natural language processing (NLP) and artificial intelligence (AI)-driven features Keel Mind supports clinician decision-making to meet the growing mental health needs of the population. Keel Mind functions to provide accessible service, efficiently train high-quality clinicians and enhance clinical outcomes for clients. In a preliminary study within post-secondary students from Ontario, Canada, 90.1% of clients reported liking the platform, and 83.4% reported that they experienced positive mental health outcomes as a result of using Keel Mind.

Practical implications
Keel Mind is a leader within the rapidly developing field of telepsychology and aims to promote positive mental health outcomes worldwide.

Originality/value
This digital showcase is the first published piece of work highlighting the technological capabilities of Keel Mind as a digital therapeutic platform. The intention of this work is to highlight current uses of digital technology within the field of therapeutic practice and to promote technological growth within the field.
Development of a Genetic Algorithm – Artificial Neural Network model to optimize the Dimensional Accuracy of parts printed by FFF
Authors: Hashemi Baghi Ali , Mansour Jasmin 
Published: April 2024

PurposeFused Filament Fabrication (FFF) is one of the growing technologies in additive manufacturing, that can be used in a number of applications. In this method, process parameters can be customized and their simultaneous variation has conflicting impacts on various properties of printed parts such as dimensional accuracy (DA) and surface finish. These properties could be improved by optimizing the values of these parameters.Design/methodology/approachIn this paper, four process parameters, namely, print speed, build orientation, raster width, and layer height which are referred to as “input variables” were investigated. The conflicting influence of their simultaneous variations on the DA of printed parts was investigated and predicated. To achieve this goal, a hybrid Genetic Algorithm – Artificial Neural Network (GA-ANN) model, was developed in C#.net, and three geometries, namely, U-shape, cube and cylinder were selected. To investigate the DA of printed parts, samples were printed with a central through hole. Design of Experiments (DoE), specifically the Rotational Central Composite Design method was adopted to establish the number of parts to be printed (30 for each selected geometry) and also the value of each input process parameter. The dimensions of printed parts were accurately measured by a shadowgraph and were used as an input data set for the training phase of the developed ANN to predict the behavior of process parameters. Then the predicted values were used as input to the Desirability Function tool which resulted in a mathematical model that optimizes the input process variables for selected geometries. The mean square error of 0.0528 was achieved, which is indicative of the accuracy of the developed model.FindingsThe results showed that print speed is the most dominant input variable compared to others, and by increasing its value, considerable variations resulted in DA. The inaccuracy increased, especially with parts of circular cross section. In addition, if there is no need to print parts in vertical position, the build orientation should be set at 0° to achieve the highest DA. Finally, optimized values of raster width and layer height improved the DA especially when the print speed was set at a high value.Originality/valueBy using ANN, it is possible to investigate the impact of simultaneous variations of FFF machines’ input process parameters on the DA of printed parts. By their optimization, parts of highly accurate dimensions could be printed. These findings will be of significant value to those industries that need to produce parts of high DA on FFF machines.
Leveraging liminality: how the interim manager’s liminal position facilitates knowledge transfer to client organizations
Authors: Rubin Viktoria 
Published: April 2024

Purpose
With the rise of the gig economy, management positions are increasingly staffed with flexible labor, so-called interim managers. They plunge into organizations for a limited period, operating in a liminal position as partly insider, partly outsider. Although several contributions to their client organizations are acknowledged, it is unknown how the interim manager’s knowledge from previous assignments is made useful in the new context under these particular working conditions. Therefore, the purpose of this paper is to increase the understanding of how the interim manager’s knowledge is transferred to the client organization while operating from a liminal position.

Design/methodology/approach
This paper presents an interview-based multiple case study of six interim assignments where knowledge transfer is considered a social and context-dependent process.

Findings
The findings unveil the multifaceted nature of the liminal position, which consists of task orientation, time limitation, political detachment and cultural distance. These facets contribute to knowledge transfer in terms of new shared understandings and joint interests, which in turn might create new practices that augment continuous knowledge-sharing patterns.

Originality/value
The results contribute to the research on flexible work arrangements by shedding light on how the liminal position, predominantly depicted as an obstacle for the individual, might facilitate knowledge transfer. Through the process of knowledge generation, it is shown how a short-term engagement might enable the organization to increase its knowledge over time.
A self‐supervised scheme for ground roll suppression
Authors: Liu Sixiu , Birnie Claire , Bakulin Andrey , Dawood Ali , Silvestrov Ilya , Alkhalifah Tariq 
Published: April 2024

AbstractIn recent years, self‐supervised procedures have advanced the field of seismic noise attenuation, due to not requiring a massive amount of clean labelled data in the training stage, an unobtainable requirement for seismic data. However, current self‐supervised methods usually suppress simple noise types, such as random and trace‐wise noise, instead of the complicated, aliased ground roll. Here, we propose an adaptation of a self‐supervised procedure, namely, blind‐fan networks, to remove aliased ground roll within seismic shot gathers without any requirement for clean data. The self‐supervised denoising procedure is implemented by designing a noise mask with a predefined direction to avoid the coherency of the ground roll being learned by the network while predicting one pixel's value. Numerical experiments on synthetic and field seismic data demonstrate that our method can effectively attenuate aliased ground roll.
Integrated non‐targeted metabolomics and transcriptomics reveals the browning mechanism of scraped ginger (Zingiber officinale Rosc.)
Authors: Tong Moru , Ding Yangfei , YU Hao , Zhang Wei , Wu Deling 
Published: April 2024

AbstractGinger (Zingiber officinale Rosc.) possesses a rich nutritional profile, making it a valuable ingredient for a wide range of culinary applications. After removing its outer skin, ginger can be effectively utilized in the production of pickles and other processed food products. However, following scraping, ginger undergoes a series of physiological and biochemical changes during storage, which can impact its subsequent development and utilization in food. Thus, the current study aimed to investigate the browning mechanism of scraped ginger using non‐targeted metabolomics and transcriptomics. The findings revealed 149 shared differential metabolites and 639 shared differential genes among freshly scraped ginger, ginger browned for 5 days, and ginger browned for 15 days. These metabolites and genes are primarily enriched in stilbenes, diarylheptane, and gingerol biosynthesis, phenylpropanoid biosynthesis, and tyrosine metabolism. Through the combined regulation of these pathways, the levels of phenolic components (such as chlorogenic acid and ferulic acid) and the ginger indicator component (6‐gingerol) decreased, whereas promoting an increase in the content of coniferaldehyde and curcumin. Additionally, the activities of polyphenol oxidase (PPO) and peroxidase (POD) were significantly increased (p‐adjust <0.05). This study hypothesized that chlorogenic and ferulic acid undergo polymerization under the catalysis of PPO and POD, thereby exacerbating the lignification of scraped ginger. These findings offer a theoretical foundation for understanding the browning mechanism of ginger after scraping.Practical ApplicationGinger's quality and nutrition can change when its skin is removed. This happens due to physical and biochemical reactions during scraping. The browning that occurs affects both the taste and health benefits of ginger, we can better understand how to prevent browning and maintain ginger's quality. This research sheds light on improving ginger processing techniques for better products.
Effect of cellulase on dough structure and quality characteristics of tough biscuits enriched with potato whole flour
Authors: Liu Xinhua , Zhu Xiao , Zhu Ying , Wang Chenjie , Li Hongjun , Chen Shanfeng 
Published: April 2024

AbstractPotato whole flour is a promising way to improve the nutrition of tough biscuits, while its gluten‐free characteristic was difficult to form acceptable texture properties. In this study, cellulase was used to degrade the cellulose in dough enriched with potato whole flour, so as to mitigate the interference of cellulose with the gluten network, resulting in forming the potato whole flour biscuit with great characteristics. Results indicated that cellulase within 0.2% led to the gradually reduced G' and G’’ values of dough from 5.50×104 to 4.00×104 and 2.66×104 to 1.35×104, respectively. Cellulase at 0.2% resulted in the significantly increased tensile properties of the dough compared to the control. The incorporation of cellulase within 0.2% also led to the tightly ordered and intact network structure base on the results of SEM, disulfide bonds determination and FTIR. Those results indicated that cellulase was beneficial to improve the baking quality of dough, which was conductive to form tough biscuit with great characteristics. The hardness, crunchiness, crispness and specific volume analysis results confirmed that 0.2% cellulase resulted in the significantly decreased hardness by 45.25% and the significantly increased specific volume, crunchiness and crispness by 24.74%, 121.20% and 156.47%, respectively. Overall, cellulase ultimately improved the quality of the biscuits by improving the properties and structure of the dough. It was of great significance for the utilization of potato whole flour resources and the industrial production of its tough biscuits.Practical ApplicationThe results showed that inclusion of cellulase led to the reduced hardness and increased crunchiness, crispness, and specific volume of potato whole flour tough biscuits. Cellulase could be used as a potential improver of tough biscuits. This study will provide guidance for practical uses of cellulase in improving potato whole flour dough and tough biscuit quality.
Applications of high‐intensity ultrasound on shrimp: Potential, constraints, and prospects in the extraction and retrieval of bioactive compounds, safety, and quality
Authors: Marques Lucas P. , Bernardo Yago A. A. , Conte‐Junior Carlos A. 
Published: April 2024

AbstractThe global shrimp market holds substantial prominence within the food industry, registering a significant USD 24.7 billion in worldwide exportation in 2020. However, the production of a safe and high‐quality product requires consideration of various factors, including the potential for allergenic reactions, occurrences of foodborne outbreaks, and risks of spoilage. Additionally, the exploration of the recovery of bioactive compounds (e.g., astaxanthin [AX], polyunsaturated fatty acids, and polysaccharides) from shrimp waste demands focused attention. Within this framework, this review seeks to comprehend and assess the utilization of high‐intensity ultrasound (HIUS), both as a standalone method and combined with other technologies, within the shrimp industry. The objective is to evaluate its applications, limitations, and prospects, with a specific emphasis on delineating the impact of sonication parameters (e.g., power, time, and temperature) on various applications. This includes an examination of undesirable effects and identifying areas of interest for current and prospective research. HIUS has demonstrated promise in enhancing the extraction of bioactive compounds, such as AX, lipids, and chitin, while concurrently addressing concerns such as allergen reduction (e.g., tropomyosin), inactivation of pathogens (e.g., Vibrio parahaemolyticus), and quality improvement, manifesting in reduced melanosis scores and improved peelability. Nonetheless, potential impediments, particularly related to oxidation processes, especially those associated with lipids, pose a hindrance to its widespread implementation, potentially impacting texture properties. Consequently, further optimization studies remain imperative. Moreover, novel applications of sonication in shrimp processing, including brining, thawing, and drying, represent a promising avenue for expanding the utilization of HIUS in the shrimp industry.