bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation on the Therapeutic Effect of Polygonum multiflorum Thunb. in Chronic Stress-Induced Hair Loss in Mice Coupled with Metabolomics and Proteomics
Authors: Zhao Yong-Kang , Guo Rui-Xian , Li Rui-Sheng , Shi Wei , Gong Hai-Ying , Ma Run-Ran , Gao Han , Li Zhen , Hu Ke-Jian , Bai Zhao-Fang , Xiao Xiao-He , Zang Qing-Ce , Kong Wei-Jun , Li Peng , Niu Ming , Wang Jia-Bo , Gao Yuan 
Published: May 2024

Objective:
Stress is the main factor that leads to hair loss, and no effective therapeutics have been developed for stress alopecia. Polygonum multiflorum Thunb. (PM) is a famous traditional Chinese medicine for tonifying the kidney and nourishing the liver, which is used to treat hair loss. This study aimed to demonstrate that PM is a promising agent for the treatment of chronic stress-induced hair loss.

Materials and Methods:
C57BL/6J male mice were subjected to chronic restraint stress to inhibit hair follicle growth. Subsequently, the effect of PM was examined by oral administration of the PM water extract or cortisone, followed by histological analysis, immunofluorescence of follicle samples, serum metabolomics, and adrenal proteomics.

Results:
Hair regeneration in a mouse model was severely inhibited by chronic restraint stress. After PM treatment, hair regeneration in model mice was promoted, including an increased length of hair shafts in skin sections, an increased number of 5-Ethynyl-2’-deoxyuridine-positive cells, and a decreased number of apoptotic cells around the hair follicles. The various test indicators of these PM-treated mice almost recovered to levels comparable to those of the control mice. Through metabolomics and proteomics analyses, we identified 181 endogenous differential metabolites and 75 differential proteins, which may be the potential targets for PM. Pathway enrichment analysis showed that steroid biosynthesis (the main pathway through which stress leads to an imbalance in glucocorticoid secretion by the adrenal gland) may play an important role in the therapeutic effects of PM. In addition, omics analysis revealed that glycolysis/gluconeogenesis, pyruvate metabolism, lipid metabolism, and other glucocorticoid-related metabolic pathways differed significantly after PM treatment.

Conclusions:
PM is an important natural drug that regulates hair regeneration in mice under stress, and its underlying mechanism is closely related to the regulation of adrenal glucocorticoids and their metabolism.
GLI1 and PTTG1 expression in colorectal carcinoma patients undergoing radical surgery and their correlation with lymph node metastasis
Authors: Cao Feng , Chen Yang-Yan , Wang Hong-Cheng 
Published: May 2024

BACKGROUND
Few studies have investigated the expression of GLI1 and PTTG1 in patients undergoing radical surgery for colorectal carcinoma (CRC) and their association with lymph node metastasis (LNM). Therefore, more relevant studies and analyses need to be conducted.
AIM
To explore GLI1 and PTTG1 expression in patients undergoing radical surgery for CRC and their correlation with LNM.
METHODS
This study selected 103 patients with CRC admitted to our hospital between April 2020 and April 2023. Sample specimens of CRC and adjacent tissues were collected to determine the positive rates and expression levels of GLI1 and PTTG1 . The correlation of the two genes with patients’ clinicopathological data (e.g., LNM) was explored, and differences in GLI1 and PTTG1 expression between patients with LNM and those without were analyzed. Receiver operating characteristic (ROC) curves were plotted to evaluate the predictive potential of the two genes for LNM in patients with CRC.
RESULTS
Significantly higher positive rates and expression levels of GLI1 and PTTG1 were observed in CRC tissue samples compared with adjacent tissues. GLI1 and PTTG1 were strongly linked to LNM in patients undergoing radical surgery for CRC, with higher GLI1 and PTTG1 levels found in patients with LNM than in those without. The areas under the ROC curve of GLI1 and PTTG1 in assessing LNM in patients with CRC were 0.824 and 0.811, respectively.
CONCLUSION
GLI1 and PTTG1 expression was upregulated in patients undergoing radical surgery for CRC and are significantly related to LNM in these patients. Moreover, high GLI1 and PTTG1 expression can indicate LNM in patients with CRC undergoing radical surgery. The expression of both genes has certain diagnostic and therapeutic significance.
Evaluating effectiveness and safety of combined percutaneous transhepatic gallbladder drainage and laparoscopic cholecystectomy in acute cholecystitis patients: Meta-analysis
Authors: Li Yu , Xiao Wei-Ke , Li Xiao-Jun , Dong Hui-Yuan 
Published: May 2024

BACKGROUND
Acute cholecystitis (AC) is a common disease in general surgery. Laparoscopic cholecystectomy (LC) is widely recognized as the "gold standard" surgical procedure for treating AC. For low-risk patients without complications, LC is the recommended treatment plan, but there is still controversy regarding the treatment strategy for moderate AC patients, which relies more on the surgeon's experience and the medical platform of the visiting unit. Percutaneous transhepatic gallbladder puncture drainage (PTGBD) can effectively alleviate gallbladder inflammation, reduce gallbladder wall edema and adhesion around the gallbladder, and create a "time window" for elective surgery.
AIM
To compare the clinical efficacy and safety of LC or PTGBD combined with LC for treating AC patients, providing a theoretical basis for choosing reasonable surgical methods for AC patients.
METHODS
In this study, we conducted a clinical investigation regarding the combined use of PTGBD tubes for the treatment of gastric cancer patients with AC. We performed searches in the following databases: PubMed, Web of Science, EMBASE, Cochrane Library, China National Knowledge Infrastructure, and Wanfang Database. The search encompassed literature published from the inception of these databases to the present. Subsequently, relevant data were extracted, and a meta-analysis was conducted using RevMan 5.3 software.
RESULTS
A comprehensive analysis was conducted, encompassing 24 studies involving a total of 2564 patients. These patients were categorized into two groups: 1371 in the LC group and 1193 in the PTGBD + LC group. The outcomes of the meta-analysis revealed noteworthy disparities between the PTGBD + LC group and the LC group in multiple dimensions: (1) Operative time: Mean difference (MD) = 17.51, 95%CI: 9.53-25.49, P < 0.01; (2) Conversion to open surgery rate: Odds ratio (OR) = 2.95, 95%CI: 1.90-4.58, P < 0.01; (3) Intraoperative bleeding loss: MD = 32.27, 95%CI: 23.03-41.50, P < 0.01; (4) Postoperative hospital stay: MD = 1.44, 95%CI: 0.14-2.73, P = 0.03; (5) Overall postoperative complication rate: OR = 1.88, 95%CI: 1.45-2.43, P < 0.01; (6) Bile duct injury: OR = 2.17, 95%CI: 1.30-3.64, P = 0.003; (7) Intra-abdominal hemorrhage: OR = 2.45, 95%CI: 1.06-5.64, P = 0.004; and (8) Wound infection: OR = 0. These findings consistently favored the PTGBD + LC group over the LC group. There were no significant differences in the total duration of hospitalization [MD = -1.85, 95%CI: -4.86-1.16, P = 0.23] or bile leakage [OR = 1.33, 95%CI: 0.81-2.18, P = 0.26] between the two groups.
CONCLUSION
The combination of PTGBD tubes with LC for AC treatment demonstrated superior clinical efficacy and enhanced safety, suggesting its broader application value in clinical practice.
Sinomenine increases osteogenesis in mice with ovariectomy-induced bone loss by modulating autophagy
Authors: Xiao Hai-Xiang , Yu Lei , Xia Yu , Chen Kai , Li Wen-Ming , Ge Gao-Ran , Zhang Wei , Zhang Qing , Zhang Hong-Tao , Geng De-Chun 
Published: May 2024

BACKGROUND
A decreased autophagic capacity of bone marrow mesenchymal stromal cells (BMSCs) has been suggested to be an important cause of decreased osteogenic differentiation. A pharmacological increase in autophagy of BMSCs is a potential therapeutic option to increase osteoblast viability and ameliorate osteoporosis.
AIM
To explore the effects of sinomenine (SIN) on the osteogenic differentiation of BMSCs and the underlying mechanisms.
METHODS
For in vitro experiments, BMSCs were extracted from sham-treated mice and ovariectomized mice, and the levels of autophagy markers and osteogenic differentiation were examined after treatment with the appropriate concentrations of SIN and the autophagy inhibitor 3-methyladenine. In vivo , the therapeutic effect of SIN was verified by establishing an ovariectomy-induced mouse model and by morphological and histological assays of the mouse femur.
RESULTS
SIN reduced the levels of AKT and mammalian target of the rapamycin (mTOR) phosphorylation in the phosphatidylinositol 3-kinase (PI3K)/AKT/mTOR signaling pathway, inhibited mTOR activity, and increased autophagy ability of BMSCs, thereby promoting the osteogenic differentiation of BMSCs and effectively alleviating bone loss in ovariectomized mice in vivo .
CONCLUSION
The Chinese medicine SIN has potential for the treatment of various types of osteoporosis, bone homeostasis disorders, and autophagy-related diseases.
GATA binding protein 2 mediated ankyrin repeat domain containing 26 high expression in myeloid-derived cell lines
Authors: Jiang Yang-Zhou , Hu Lan-Yue , Chen Mao-Shan , Wang Xiao-Jie , Tan Cheng-Ning , Xue Pei-Pei , Yu Teng , He Xiao-Yan , Xiang Li-Xin , Xiao Yan-Ni , Li Xiao-Liang , Ran Qian , Li Zhong-Jun , Chen Li 
Published: May 2024

BACKGROUND
Thrombocytopenia 2, an autosomal dominant inherited disease characterized by moderate thrombocytopenia, predisposition to myeloid malignancies and normal platelet size and function, can be caused by 5’-untranslated region (UTR) point mutations in ankyrin repeat domain containing 26 (ANKRD26). Runt related transcription factor 1 (RUNX1) and friend leukemia integration 1 (FLI1) have been identified as negative regulators of ANKRD26 . However, the positive regulators of ANKRD26 are still unknown.
AIM
To prove the positive regulatory effect of GATA binding protein 2 (GATA2) on ANKRD26 transcription.
METHODS
Human induced pluripotent stem cells derived from bone marrow (hiPSC-BM) and urothelium (hiPSC-U) were used to examine the ANKRD26 expression pattern in the early stage of differentiation. Then, transcriptome sequencing of these iPSCs and three public transcription factor (TF) databases (Cistrome DB, animal TFDB and ENCODE) were used to identify potential TF candidates for ANKRD26 . Furthermore, overexpression and dual-luciferase reporter experiments were used to verify the regulatory effect of the candidate TFs on ANKRD26 . Moreover, using the GENT2 platform, we analyzed the relationship between ANKRD26 expression and overall survival in cancer patients.
RESULTS
In hiPSC-BMs and hiPSC-Us, we found that the transcription levels of ANKRD26 varied in the absence of RUNX1 and FLI1. We sequenced hiPSC-BM and hiPSC-U and identified 68 candidate TFs for ANKRD26 . Together with three public TF databases, we found that GATA2 was the only candidate gene that could positively regulate ANKRD26 . Using dual-luciferase reporter experiments, we showed that GATA2 directly binds to the 5’-UTR of ANKRD26 and promotes its transcription. There are two identified binding sites of GATA2 that are located 2 kb upstream of the TSS of ANKRD26 . In addition, we discovered that high ANKRD26 expression is always related to a more favorable prognosis in breast and lung cancer patients.
CONCLUSION
We first discovered that the transcription factor GATA2 plays a positive role in ANKRD26 transcription and identified its precise binding sites at the promoter region, and we revealed the importance of ANKRD26 in many tissue-derived cancers.
Hepatocyte growth factor enhances the ability of dental pulp stem cells to ameliorate atherosclerosis in apolipoprotein E-knockout mice
Authors: Duan Han , Tao Ning , Lv Lin , Yan Kai-Xin , You Yong-Gang , Mao Zhuang , Wang Chang-Yao , Li Xue , Jin Jia-Yan , Wu Chu-Tse , Wang Hua 
Published: May 2024

BACKGROUND
Atherosclerosis (AS), a chronic inflammatory disease of blood vessels, is a major contributor to cardiovascular disease. Dental pulp stem cells (DPSCs) are capable of exerting immunomodulatory and anti-inflammatory effects by secreting cytokines and exosomes and are widely used to treat autoimmune and inflammation-related diseases. Hepatocyte growth factor (HGF) is a pleiotropic cytokine that plays a key role in many inflammatory and autoimmune diseases.
AIM
To modify DPSCs with HGF (DPSC-HGF) and evaluate the therapeutic effect of DPSC-HGF on AS using an apolipoprotein E-knockout (ApoE-/-) mouse model and an in vitro cellular model.
METHODS
ApoE-/- mice were fed with a high-fat diet (HFD) for 12 wk and injected with DPSC-HGF or Ad-Null modified DPSCs (DPSC-Null) through tail vein at weeks 4, 7, and 11, respectively, and the therapeutic efficacy and mechanisms were analyzed by histopathology, flow cytometry, lipid and glucose measurements, real-time reverse transcription polymerase chain reaction (RT-PCR), and enzyme-linked immunosorbent assay at the different time points of the experiment. An in vitro inflammatory cell model was established by using RAW264.7 cells and human aortic endothelial cells (HAOECs), and indirect co-cultured with supernatant of DPSC-Null (DPSC-Null-CM) or DPSC-HGF-CM, and the effect and mechanisms were analyzed by flow cytometry, RT-PCR and western blot. Nuclear factor-κB (NF-κB) activators and inhibitors were also used to validate the related signaling pathways.
RESULTS
DPSC-Null and DPSC-HGF treatments decreased the area of atherosclerotic plaques and reduced the expression of inflammatory factors, and the percentage of macrophages in the aorta, and DPSC-HGF treatment had more pronounced effects. DPSCs treatment had no effect on serum lipoprotein levels. The FACS results showed that DPSCs treatment reduced the percentages of monocytes, neutrophils, and M1 macrophages in the peripheral blood and spleen. DPSC-Null-CM and DPSC-HGF-CM reduced adhesion molecule expression in tumor necrosis factor-α stimulated HAOECs and regulated M1 polarization and inflammatory factor expression in lipopolysaccharide-induced RAW264.7 cells by inhibiting the NF-κB signaling pathway.
CONCLUSION
This study suggested that DPSC-HGF could more effectively ameliorate AS in ApoE-/- mice on a HFD, and could be of greater value in stem cell-based treatments for AS.
Recruitment Advertisement and Selection Decisions in Nigerian Filling Stations
Authors: Ujebe Nome , Echezona Ovute Francis , Ogbonnia Chukwu-Etu 
Published: May 2024

This study on Recruitment Advertisement and Selection Decision in Filling stations was conducted specifically to ascertain the degree external recruitment advertisement affect selection decision; to determine the degree internal recruitment advertisement affect selection decision; and to discover the extent informal recruitment advertisement affect selection decision in Nigerian filling stations. A descriptive research design was adopted. The Resource-Based theory propounded by Barney (1986b) was also adopted for the study. Sixty five (65) respondents being management staff of Petroleum Filling stations responded to fifteen (15) questions. The hypotheses were tested using Regression analysis to determine the extent recruitment advertisement affect selection decision. The result showed that informal recruitment method is most effective in selecting the right workers for the right job. Therefore, the researcher recommended the use of informal recruitment method especially for vacancies required to be filled at short notice. He went further to recommend the use of external recruitment method using digital platform for vacancies that would need to be filled in a longer time. Also internal recruitment such as promotion and job rotation was recommended for adoption but that extensive use of internal recruitment may imply that fresh candidates with new knowledge will not be opportune to work in the organization.
Evaluation of mitral chordae tendineae length using four-dimensional computed tomography
Authors: Mori Takuya , Matsushita Satoshi , Morita Terumasa , Abudurezake Abulaiti , Mochizuki Junji , Amano Atsushi 
Published: May 2024

BACKGROUND
Mitral valvuloplasty using artificial chordae tendineae represents an effective surgical approach for treating mitral regurgitation. Achieving precise measurements of artificial chordae tendineae length (CL) is an important factor in the procedure; however, no objective index currently exists to facilitate this measurement. Therefore, preoperative assessment of CL is critical for surgical planning and support. Four-dimensional x-ray micro-computed tomography (4D-CT) may be useful for accurate CL measurement considering that it allows for dynamic three-dimensional (3D) evaluation compared to that with transthoracic echocardiography, a conventional inspection method.
AIM
To investigate the behavior and length of mitral chordae tendineae during systole using 4D-CT.
METHODS
Eleven adults aged > 70 years without mitral valve disease were evaluated. A 64-slice CT scanner was used to capture 20 phases in the cardiac cycle in electrocardiographic synchronization. The length of the primary chordae tendineae was measured from early systole to early diastole using the 3D image. The primary chordae tendineae originating from the anterior papillary muscle and attached to the A1-2 region and those from the posterior papillary muscle and attached to the A2-3 region were designated as cA and cP, respectively. The behavior and maximum lengths [cA (ma), cP (max)] were compared, and the correlation with body surface area (BSA) was evaluated.
RESULTS
In all cases, the mitral anterior leaflet chordae tendineae could be measured. In most cases, the cA and cP chordae tendineae could be measured visually. The mean cA (max) and cP (max) were 20.2 mm ± 1.95 mm and 23.5 mm ± 4.06 mm, respectively. cP (max) was significantly longer. The correlation coefficients (r) with BSA were 0.60 and 0.78 for cA (max) and cP (max), respectively. Both cA and cP exhibited constant variation in CL during systole, with a maximum 1.16-fold increase in cA and a 1.23-fold increase in cP from early to mid-systole. For cP, CL reached a plateau at 15% and remained elongated until end-systole, whereas for cA, after peaking at 15%, CL shortened slightly and then moved toward its peak again as end-systole approached.
CONCLUSION
The study suggests that 4D-CT is a valuable tool for accurate measurement of both the length and behavior of chordae tendineae within the anterior leaflet of the mitral valve.
Сучасна Угорщина – шлях від демократії до авторатиризму: історична ретроспектива
Authors: Ткач Д.І. 
Published: May 2024

У кінці 1980-х рр., внаслідок оксамитових революцій, політичні системи більшості країн Центральної та Східної Європи (ЦСЄ) пройшли значні зміни. Цей процес також зачіпив Угорську Республіку, де зміна суспільного устрою пройшла інтенсивніше і успішніше, ніж у багатьох інших країнах ЦСЄ.
Угорщина, завдяки цим процесам, відзначилася як лідер серед країн ЦСЄ, які досягли значних досягнень у сфері політичної та економічної трансформації. Політична система суспільства зазнала значних змін. Однопартійний режим було замінено плюралістичною політичною системою, яка базувалася на Конституції, зміненій у 1989 р. Була впроваджена парламентсько-президентська система влади, яка надала уряду широкі повноваження і зобов’язала його забезпечувати умови для економічного і демократичного розвитку країни. Також була проведена реформа судової системи, і створений Конституційний Суд.
З 1990 року до 2010 року відбулася значна лібералізація суспільства, але досягнення цих років мали суперечливий та неоднозначний характер. Комерціалізація всіх сфер життя суттєво змінила загальнокультурний контекст, призвела до нових напруженостей у відносинах між різними соціальними групами і різними поколіннями, і певною мірою загострила проблему міжнаціональних відносин.
Все змінилося в 2010 р., коли на виборах в Державні збори до влади прийшла коаліція ФІДЕС-УГП-ХДНП, отримавши конституційну більшість голосів. Ця правоконсервативна коаліція успішно впроваджувала консервативну ідеологію, вміло маніпулювала громадською свідомістю, здійснювала ефективні економічні реформи, посилювала державне управління та реформувала державний сектор, спрямовуючи його к централізації і відновленню консервативних традицій угорської держави. Це також супроводжувалося згортанням демократичних принципів правління та посиленням авторитарних тенденцій.
Внаслідок цих дій, правоконсервативна коаліція зуміла перемогти на виборах до Державних зборів у 2014, 2018 та 2022 роках. Це призвело до того, що багатопартійна система, створена як противага однопартійному тоталітарному режиму, стала двополюсною з домінуючою роллю коаліції ФІДЕС-УГП-ХДНП.
Таким чином країна із усталеними демократичними традиціями перетворилася на державу з авторитарним урядом на чолі із прем’єр-міністром В.Орбаном, якого багато хто в ЄС називає диктатором.
Introduction
Authors: Shull Kristina 
Published: May 2024

Abstract
The introduction provides an overview of the contemporary US immigration detention system and lays out the book’s main arguments by defining key concepts such as empire, resistance, counterinsurgency, the Reagan imaginary, and critical refugee studies. By analyzing various forms of public and personal resistance to immigration enforcement and detention in the 1980s, the book argues that the Reagan administration adopted a set of new immigration detention policies in alignment with Cold War foreign policy goals and theorizes that detention operates as a form of counterinsurgency under the Reagan administration’s War on Drugs. The introduction also discusses the election of Ronald Reagan in 1980 as a pivotal moment for the rise of mass incarceration in US history.
This Time, They’ll Be Feet People
Authors: Shull Kristina 
Published: May 2024

Abstract
This chapter details how the Reagan administration’s increasing US military aid and interventions in ongoing civil wars in Central America caused mass displacement of Salvadoran refugees and Guatemalan refugees fleeing US-backed violence, prompting US policy responses including detention, inter-agency law enforcement cooperation, and border militarization. Through covert military operations inspired by low-intensity conflict doctrine and drug interdiction provisions under the War on Drugs, the Reagan administration adopted a Cold War political strategy of denying the role of US-trained death squads in refugee displacement to claim that leftism and communism were the only causes of violence and refugee displacement. The chapter then describes how the Reagan administration implemented the Caribbean Basin Initiative in El Salvador with an intent of refugee deterrence, and how the State Department used the 1980 Refugee Act to issue systematic denials of Salvadoran and Guatemalan asylum claims while claiming to uphold due process. These policies sparked a growing, transnational Central American peace movement. The chapter concludes by recounting the experiences of asylum-seekers detained at the El Centro detention center in California and legal and community advocacy on their behalf. Meanwhile, the Reagan administration continued developing contingency detention plans such as Rex84 in anticipation of future mass migrations.