bluuebunny/crossref_metadata_2025_split huggingface dataset:
Postoperative patient-reported outcomes in intraoperative sensor-assisted versus manual primary total knee arthroplasty: a scoping review
Authors: Zhu Jinyu , Xie Tianshun , Chen Junjie 
Published: April 2024

Abstract

Purpose
To compare postoperative patient-reported outcomes between intraoperative sensor-assisted and manual primary total knee arthroplasty (TKA) by reviewing the available literature on this topic.
Methods
We conducted a systematic search of the PubMed and Web of Science databases from their inception until April 2023 to identify clinical studies comparing postoperative patient-reported outcomes between intraoperative sensor-assisted and manual primary TKA. We excluded nonclinical studies and conference abstracts without full-text. Joanna Briggs Institute (JBI) checklists were used for critical appraisal of the eligible clinical studies.
Results
Out of the nine clinical studies that met the predefined inclusion and exclusion criteria, six were cohorts and three were randomized controlled trials. Eight studies found no significant difference in postoperative patient-reported outcomes between intraoperative sensor-assisted and manual primary TKA. One study reported significantly higher Knee Society Score and Oxford Knee Score in sensor-assisted TKA as compared to manual primary TKA. None of the included studies satisfied all of the criteria outlined in the JBI checklists.
Conclusions
This review finds that there is no evidence to support the claim that sensor-assisted TKA is superior to manual primary TKA in terms of short-term postoperative patient-reported outcomes. Future clinical studies with larger sample sizes and long-term follow-ups are required to assess the potential benefits of intraoperative sensor-assisted TKA.
Clinical features and prognostic factors of Cardiorenal anemia syndrome in China: a retrospective single-center study
Authors: Zhu Mengyue , Liu Min , Lu Chunlei , He Dafeng , Li Jiao , Xu Xia , Cui Ying , Zhao Chuanyan , Xu Jun , Zhou Gang , Mou Hongbin , Bi Guangyu , Liu Changhua , Wang Rong 
Published: April 2024

Abstract

Background
There is little research on cardiorenal anemia syndrome (CRAS) in China. This study was to describe the characteristics of patients with CRAS and to explore predictive risk factors of all-cause mortality.
Methods
A total of 81795 patients were hospitalized from August 2012 to August 2021 in the nephrology department and cardiology department, of which 820 patients with CRAS met the inclusion criteria and were recruited into this study. The 820 patients with CRAS were divided into three groups based on New York Heart Association (NYHA) functional class: a NYHA Class II group (n = 124), a NYHA Class III group (n = 492), and a NYHA Class IV group (n = 204). Demographics and laboratory tests were collected and risk factors of death events were analyzed. The primary endpoint of the study was death.
Results
820 patients were included, with a median age of 65.00 (51.00–75.00) years and 61.2% were men. The median follow-up was 27.0 (13.0–51.0) months. 416 (50.7%) patients died during follow-up. Age, smoking history, cerebral infarction, NYHA functional class, albumin, serum creatinine (SCr), left ventricular end-diastolic diameter (LVEDD), and left ventricular ejection fraction (LVEF) remained independent predictors in patients with CRAS (P < 0.05) after adjusting to the potential confounders.
Conclusions
Heart failure and renal dysfunction are a fatal combination and are associated with poor prognosis in patients with CRAS.
Mean Performance and Stability of Open Pollinated Tomato Varieties in Multi-Environment Trials: Selection Based on Yield
Authors: Masoodi Ummyiah H , Shah Immad A 
Published: April 2024

Abstract

Additive main effect and multiplicative interaction analysis revealed significant impact of environment (E), and consequently a significant GE interaction of tomato genotypes at major locations of Kashmir Division. The environment (E), genotype (G), and GEI significantly affect the plant yield/ha (p < 0.001). The environment explains approximately 47.5% of the total sum of squares due to treatments (G + E+GEI). Assuming the weight of 50:50 (i.e. having equal importance for plant yield and stability), Arka Meghali, NDF-9, VTG-93 had high values for WAAS and were the stable genotypes. The plot based on the MTSI shows the rankings of the genotypes as per the multi-trait stability index and indicates Arka Meghali and NDF-9 as the selected genotypes. The evaluation based on various stability indicators are in agreement with the results from the Multi Trait Stability Index. This study underscores the significance of understanding the complex interplay between genotype, environment, and their interaction on crop yield, providing valuable insights for tomato cultivar selection and agricultural practices in the ecologically important region of Northern Himalayas.
Lymphocyte Antigen 6 Family Member D (LY6D) affects stem cell phenotype and progression of pancreatic adenocarcinoma
Authors: Uemura Mamoru , Takahashi Hidekazu , Nishida Naohiro , Mori Masaki , Yokoyama Yuhki , Yamamoto Hiroyuki , Hamabe Atsushi , yamamoto Hirofumi , Miyoshi Norikatsu , Takahashi Hidenori , Doki Yuichiro , Eguchi Hidetoshi , Kobayashi Shogo , Ogino Takayuki , Okimura Shumpei 
Published: April 2024

Abstract

The membrane-bound protein Lymphocyte Antigen 6 Family Member D (LY6D) has been implicated in stemness and is associated with various tumors. LY6D is specifically expressed in cancer cells, with little expression in normal cells, but its physiological function in cancer progression remains poorly understood. Here, we investigated how LY6D was associated with pancreatic ductal adenocarcinoma (PDAC), and involved in stemness. We conducted functional analysis of LY6D to evaluate its contribution to impacting PDAC cells in vitro. Using our in-house developed stem cell separation technique, which can isolate cells with low proteasome activity and high stem cell properties, we successfully enriched cells with extremely high tumorigenic ability. We then used these cells, to perform in vitro functional assays by manipulating the LY6D expression. Immunohistopathological analysis was also conducted to reveal the clinical significance of LY6D in PDAC. In vitro functional assays demonstrated that LY6D was crucially involved in the cancer malignant phenotype including invasive ability, drug resistance, migration abilities, and cancer stemness. Immunohistopathological analysis revealed that high LY6D expression levels were associated with high recurrence rate and poorer prognosis in PDAC. Our present findings demonstrated that LY6D plays a key role in regulation of cancer stem cells. LY6D is not only a prognostic indicator but also could be a promising therapeutic target in PDAC.
Development of mirror-image monobodies targeting the oncogenic BCR::ABL1 kinase
Authors: Hantschel Oliver , Schmidt Nina , Kumar Amit , Korf Lukas , Dinh-Fricke Adrian Valentin , Abendroth Frank , Koide Akiko , Rakwalska-Bange Madalena , Essen Lars , Koide Shohei , Vázquez Olalla 
Published: April 2024

Abstract

Mirror-image proteins, which are composed of d-amino acids, are an attractive therapeutic modality, as they exhibit high metabolic stability and lack immunogenicity. Development of mirror-image binding proteins is achieved through chemical synthesis of the d-target protein, phage display library selection of an l-binder and chemical synthesis of the (mirror-image) d-binder that consequently binds the physiological l-target. Monobodies are among the most well-established synthetic (l-)binding proteins and their small size (~90 residues) and lack of endogenous cysteine residues make them particularly accessible to chemical synthesis. Here we developed monobodies with nanomolar binding affinities against the d-SH2 domain of the leukemic tyrosine kinase BCR::ABL1. Two crystal structures of heterochiral monobody-SH2 complexes revealed targeting of the pY binding pocket by an unconventional binding mode. We then prepared stable and potent d-monobodies by either ligating two chemically synthesized d-peptides or by self-assembly without ligation. Their proper folding and stability were determined and high affinity binding to the l-target was shown. d-monobodies were protease-resistant, showed long-term plasma stability, inhibited BCR::ABL1 kinase activity and bound BCR::ABL1 in cells. Hence, we demonstrate that functional d-monobodies can be developed readily, enabling their use as future d-protein therapeutics to target a broad spectrum of protein-protein interactions.
Haptic experience to significantly motivate anatomy learning in medical students
Authors: Manrique Martha , Mondragón Iván , Flórez Leonardo , Montoya Luisa , García Ananías , Mera Carmen Alicia , Kuhlmann Angelika , Guillén Fabricio , Cortés Michelle , Gómez María Lucía Gutiérrez 
Published: April 2024

Abstract

Background
Currently, multiple tools exist to teach and learn anatomy, but finding an adequate activity is a challenge. However, it can be achieved through haptic experiences, where motivation is the means of a significant learning process. This study aimed to evaluate a haptic experience to determine if a tactile and painting with color marker interactive experience established a better learning process in comparison to the traditional 2D workshop on printed paper with photographs.
Methods
In this descriptive cross-sectional study, plaster bone models were elaborated from a computerized scan tomography. Second year undergraduate medical students were divided into two groups: one using the traditional 2D method and the second with 3D plaster bone models to answer the same workshop. A third group of students decided not to participate in the workshops. Following, all three groups were evaluated on bone accidents and view, laterality, muscle insertions and functions. 2D and 3D workshop students were asked their opinion in a focous group and answered a survey regarding the overall perception and learning experience. Evaluation grades are presented as mean ± standard deviation, and answers from the survey are presented as percentages.
Results
The survey demonstrated the students in the 3D model graded the experience as outstanding, and in five out of the six questions answers were very good or excellent. In contrast, for students participating in the 2D workshop the most common answers were fair or good. The exception was the answer regarding the quiz, where both groups considered it good, despite the average among all groups was not a passing grade.
Conclusions
From this study comparing the conventional methodology to learn the anatomy of the shoulder in contrast with a haptic experience with plaster bone models using touch and painting over the structures, the students described it as an interactive experience where the sense of touch greatly contributed to their learning process. Even though this activity was useful in terms of learning bone accidents and muscle origins and insertions, further activities must be developed to increase their understanding regarding their function and its relevance in a clinical setting.
Influence of IgG+ serum on Zika Virus infection response in a model of neural precursor cells
Authors: Plentz Ismael , Pazzin Douglas , Previato Thales , Wagner Fernanda , Boff Marina , Fernandes Larissa , Gonçalvez João , Marinowic Daniel , Costa Jaderson 
Published: April 2024

Abstract

Background
The high frequency of Zika virus (ZIKV) infection prompted the World Health Organization (WHO) to declare it an emerging threat in 2016. Presently, countries in South America continue to report a significant number of Zika virus cases. Zika virus infection has been associated with neurological diseases. This article explores the impact of ZIKV infection on IgG + Zika-reactive conditions using a neurosphere model.
Methods
Neurospheres derived from Wistar rats were exposed to Zika virus and IgG + Zika-reactive conditions. The study assessed the area, irregularity rate, and relative gene expression of NOTCH-1, HES-1, and HEY-1, as well as the expression of GCSF and IL-10.
Results
The irregularity rate of ZIKV neurospheres decreased at its lowest concentration; however, our morphology analysis was insufficient to fully elucidate the impact of ZIKV on neurospheres. Interestingly, IgG + serum exhibited neuroprotective effects against subsequent Zika virus exposure, restoring NOTCH-1 and HES-1 expression levels to normal. HEY-1 expression remained unaffected by Zika virus exposure but decreased with IgG + serum. Surprisingly, levels of the anti-inflammatory markers GCSF and IL-10 showed no significant changes.
Conclusions
These findings highlight the complex interplay between ZIKV infection, immune response, and neurodevelopmental processes. Further research is necessary to elucidate the precise mechanisms underlying these observations and explore potential therapeutic interventions.
Effects of Height and Number of Bays on the Seismic Behavior Factor of R.C. Moment-Resisting Frame Structures Including Soil-Structure Interaction
Authors: Oumenour Touati Radia , Mouloud OUANANI , Amar Louzai 
Published: April 2024

Abstract

In this study, the impacts of structure height and number of bays on the seismic behavior factor R of reinforced concrete (RC) moment-resistant frames are investigated with the consideration of soil-structure interaction (SSI) effects. For this purpose, 2-, 5-, 8- and 11-storey RC moment-resisting frame structures with the variation of the number of bays for each structure height are selected and designed according to the Algerian seismic code RPA 99/Version 2003. Three different soil deposit classes suggested by ATC 40and FEMA 356 are taken as reference to choose the mechanical properties of the soil. Fixed-base models and soil-structure interaction models using the substructure approach are analyzed by performing non-linear static pushover analyses in order to compute the R factor components, such as ductility and overstrength factors, with the consideration of failure criteria at both member and structural levels. Furthermore, to highlight the effect that could have the mechanical response of the soil on the R factor, two different soil responses are adopted, namely, elastic linear and elastoplastic nonlinear consecutive models. The results show that, for fixed-based models, the value of the seismic behavior factor decreases as the structure height increases, whereas for the number of bays parameter, the R factor seems to not be influenced at all. For the flexible base models, the tendency observed is the same as in fixed-base models, but with substantially lower values of R, particularly in soft soil with an elastoplastic nonlinear consecutive model. This implies that SSI has detrimental effects for RC frame structures under seismic action. These observations are extremely significant for most seismic design codes, which at present do not prescribe any link between the seismic behavior factor intended to represent structural ductility and the SSI.
Brain functional networks and executive functions in children with attention- deficit/hyperactivity disorder
Authors: Yang Hua , Yang Yue , Wang Anqi , Yang Jie , Yang Xiaowen , Zhou Jielan , Yu Tao , Liu Hao , Luo Rong 
Published: April 2024

Abstract

Brain region dysfunctions associated with executive function abnormalities may contribute to attention-deficit/hyperactivity disorder (ADHD) pathogenesis. We explored neural mechanisms through electroencephalography (EEG) studies of executive function and functional brain networks in children with ADHD. Executive function data were collected and resting-state EEG was measured in 84 children with ADHD and 84 healthy children. Functional connectivity was assessed across all scalp channels in five frequency bands. Brain networks were constructed, and relevant metrics were calculated using graph theory. Children with ADHD show varied executive function deficits. Connectivity in the frontal and parietal regions was reduced in both the eyes-open and eyes-closed states, particularly in the beta and gamma bands. Brain networks differed significantly in the beta band. Reduced characteristic path length (CPL) was seen in the eyes-closed state; global efficiency increased and CPL, clustering coefficient, and local efficiency decreased in the eyes-open state. Functional networks in children with ADHD correlate with executive function. Altered EEG connectivity and brain network topology may be underlying neural mechanisms of ADHD. Thus, EEG network dysfunction could be a potential biomarker or treatment target for future research. This study provides new insights into the underlying mechanisms of ADHD through EEG-based functional network analysis.
The Casual Relationship between Asthma-related Pneumonia and Heart Failure -- a Bidirectional Two-sample Mendelian Randomization Study
Authors: Wu Shirong , Liu Qingyan , Zheng Danwen , Liu Yuntao , Ding Banghan , Xi Xiaotu , Deng Qiuying , Liu Qing , Zhang Zhongde , Yang Rongyuan 
Published: April 2024

Abstract

Background: Observational studies have suggested a higher risk of heart failure in individuals with asthma-related pneumonia. However, there is currently no conclusive evidence establishing a causal relationship between the two or a clear mechanism explaining this association.
Methods: We performed a thorough bidirectional two-sample Mendelian randomization (MR) analysis to explore the causal connection between Asthma-related pneumonia and Heart failure. Our study employed five MR methods, including inverse variance weighted (IVW), MR-Egger, weighted median, weighted mode, and simple mode. To ensure the reliability of our findings, we conducted sensitivity analyses using different models and testing methods to evaluate the potential effects of heterogeneity and pleiotropy on the outcomes. Our results were consistently robust through these rigorous evaluations.
Results: According to the results of IVW analysis, there is a causal relationship between Asthma-related pneumonia and Heart failure risk (OR=1.113, 95%CI: 1.027~1.206, P=0.009). On the other hand, osteoporosis was not found to have a causal effect on PBC risk based on IVW analysis (OR=1.018, 95%CI: 0.780~1.328, P=0.893). The IVs used in all MR analyses showed no heterogeneity or horizontal pleiotropy. Moreover, the leave-one-out analysis revealed that no single SNP disproportionately influenced the results, further confirming the credibility of the bidirectional MR findings.
Conclusion: Through this study, it has been established that there is a causal relationship between pneumonia related to Asthma and the risk of heart failure. However, no definitive causal link has been found from heart failure to Asthma-related pneumonia. These insightful findings provide guidance for effectively managing the health of Asthma-related pneumonia with heart failure.
‘A lightbulb moment’: carers’ experiences of behavioural symptoms in motor neurone disease before and after MiNDToolkit
Authors: Mioshi Eneida , Heal Sue , Katangwe-Chigamba Thando 
Published: April 2024

Abstract

Background: to explore carers’ experiences of behavioural symptoms in Motor Neurone Disease (MND), before and after using the MiNDToolkit, a novel online psychoeducational intervention to support management of behavioural symptoms (BehSymp) in MND. The study also investigated carers’ views and acceptability of MiNDToolkit.
Methods: A qualitative process evaluation of carers engagement with, and acceptability of, the MiNDToolkit conducted using semi-structured interviews with carers (n=11). All interviews were audio-recorded, professionally transcribed verbatim and analysed thematically.
Results: five themes were identified: 1) In the dark – carers’ experiences and reactions to BehSymp; 2) Others can see: the role of HCPs in identifying symptoms – and perceived opportunities for carers to receive support; 3) Shedding light: carers implementation and perceived impact of the MiNDToolkit content; 4) Acceptability and carers’ engagement with MiNDToolkit; 5) future implementation. Carers’ experience of BehSymp was particularly distressing when symptoms were apparently out of context. MiNDToolkit appeared to support learning that BehSymp were part of MND. Content resonated with carers, who reported learning about the full picture of MND, which led to acceptance and use of newly learned strategies. Engagement with the platform was good, with varied input from HCPs. Greater and nuanced involvement from HCPs seem important to support management of BehSymp. Recommendations for a full-scale trial emerged, including adding a paper booklet to accompany the intervention and creation of new modules on emotional lability, changes in relationships, and transitioning to a care home.
Conclusions: MiNDToolkit was acceptable to carers overall. Recommended improvements should be actioned in a full-scale trial.
Postoperative Outcomes of Fontan Operation in a Multicenter Italian Study. How Far Have We Gone?
Authors: Cao Irene , Bergonzoni Emma , Vedovelli Luca , Guerra Giulia , Galletti Lorenzo , Butera Gianfranco , Trezzi Matteo , Panebianco Mario , Gargiulo Gaetano D. , Angeli Emanuela , Careddu Lucio , Zanoni Rossana , Napoleone Carlo Pace , Orsola Luca , Guariento Alvise , Scattolin Fabio , Giamberti Alessandro , Rito Mauro Lo , Marianeschi Stefano M. , Agati Salvatore , Bellanti Ermanno , Vairo Ugo , Meliota Giovanni , Scalzo Gabriele , Scrascia Giuseppe , Nuri Halkawt , Michielon Guido , Biffanti Roberta , Gozzi Anna , Salvo Giovanni , Vida Vladimiro L. , Padalino Massimo A. 
Published: April 2024

Abstract

Background: Despite the clinical results of the Fontan operation have certainly improved, it still presents with an inherent surgical risk of death and early morbidities.
Methods: This is a retrospective clinical study of children undergoing Fontan operation in 9 congenital cardiac centers in Italy between 1990 and 2023. Clinical and surgical data were collected via a dedicated RedCap database.
Results: In the last 3 decades, there were 897 patients undergoing Fontan operation, M/F 512/384, median age: 4.5 years (IQR 3.3 -6.4), median weight 16 kg (IQR 14-22). A first palliation was deemed necessary in 710 patients (80%), and most patients underwent a staged Fontan (93%); an extracardiac conduit was used in 790 patients (88%). Postoperative complications (mild to severe) occurred in 410 patients (46%), and early reinterventions were required in 66 patients (7.5%). Overall operative mortality was 1.7% (15 patients). Age at Fontan greater than 4 years was associated with an early need for transcatheter reintervention (adj p-value=0.037) and a higher incidence of postoperative complications (adj p-value=0.017).
Conclusion: The Fontan operation has seen significant improvements in immediate outcomes, notably a remarkable reduction in overall mortality to just 1.35% in the last decade. While minor complications have remained steady, there has been a substantial decrease in major early complications, deaths, and the need for reinterventions. Notably, patients aged over 4 years seem to face a higher risk of postoperative morbidity, underscoring the critical role of age in preoperative assessment and management strategies for Fontan patients.