bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Molecular and Physiological Responses ofPhyscomitrella patensto Ultraviolet-B Radiation
Authors: Wolf Luise , Rizzini Luca , Stracke Ralf , Ulm Roman , Rensing Stefan A. 
Published: April 2010

AbstractUltraviolet-B (UV-B) radiation present in sunlight is an important trigger of photomorphogenic acclimation and stress responses in sessile land plants. Although numerous moss species grow in unshaded habitats, our understanding of their UV-B responses is very limited. The genome of the model moss Physcomitrella patens, which grows in sun-exposed open areas, encodes signaling and metabolic components that are implicated in the UV-B response in flowering plants. In this study, we describe the response of P. patens to UV-B radiation at the morphological and molecular levels. We find that P. patens is more capable of surviving UV-B stress than Arabidopsis (Arabidopsis thaliana) and describe the differential expression of approximately 400 moss genes in response to UV-B radiation. A comparative analysis of the UV-B response in P. patens and Arabidopsis reveals both distinct and conserved pathways.
Induction of bone formation by transforming growth factor‐β2 in the non‐human primate Papio ursinus and its modulation by skeletal muscle responding stem cells
Authors: Ripamonti U. , Roden L. C. 
Published: April 2010

AbstractObjectives:  Four adult non‐human primates Papio ursinus were used to study induction of bone formation by recombinant human transforming growth factor‐β2 (hTGF‐β2) together with muscle‐derived stem cells.Materials and methods:  The hTGF‐β2 was implanted in rectus abdominis muscles and in calvarial defects with and without addition of morcellized fragments of striated muscle, harvested from the rectus abdominis or temporalis muscles. Expression of osteogenic markers including osteogenic protein‐1, bone morphogenetic protein‐3 and type IV collagen mRNAs from generated specimens was examined by Northern blot analysis.Results:  Heterotopic intramuscular implantation of 5 and 25 μg hTGF‐β2 combined with 100 mg of insoluble collagenous bone matrix yielded large corticalized mineralized ossicles by day 30 with remodelling and induction of haematopoietic marrow by day 90. Addition of morcellized rectus abdominis muscle to calvarial implants enhanced induction of bone formation significantly by day 90.Conclusions:  In Papio ursinus, in marked contrast to rodents and lagomorphs, hTGF‐β2 induced large corticalized and vascularized ossicles by day 30 after implantation into the rectus abdominis muscle. This striated muscle contains responding stem cells that enhance the bone induction cascade of hTGF‐β2. Induction of bone formation by hTGF‐β2 in the non‐human primate Papio ursinus may occur as a result of expression of bone morphogenetic proteins on heterotopic implantation of hTGF‐β2; the bone induction cascade initiated by mammalian TGF‐β proteins in Papio ursinus needs to be re‐evaluated for novel molecular therapeutics for induction of bone formation in clinical contexts.
Soluble factors from ASCs effectively direct control of chondrogenic fate
Authors: Kim B.‐S. , Kang K.‐S. , Kang S.‐K. 
Published: April 2010

AbstractBackground and objectives:  Adipose tissue‐derived stem cells (ASCs) have great potential for regenerative medicine. For molecular understanding of specific functional molecules present in ASCs, we analysed 756 proteins including specific chondrogenic functional factors, using high‐throughput nano reverse‐phase liquid chromatography–electrospray ionization–tandem mass spectrometry.Materials, methods and results:  Of these proteins, 33 were identified as chondrogenic factors or proteins including type 2 collagen, biglycan, insulin‐like growth factor‐binding protein and transforming growth factor‐beta 1 (TGF‐β1). ASCs are a possible cell source for cartilage regeneration as they are able to secrete a number of functional cytokines including chondrogenesis‐inducing molecules such as TGF‐β1 and bone morphogenetic protein 4 (BMP4). The chondrogenic phenotype of cultured ASCs was effectively induced by ASC‐culture media (CM) containing BMP4 and TGF‐β1, and maintained after pre‐treatment for 14 days in vitro and subcutaneous implantation in vivo. Chondrogenic differentiation efficiency of cultured ASCs and cultured mouse skin‐derived progenitor cells (SPCs) depended absolutely on ASC CM‐fold concentration. Cell density was also a very important factor for chondrogenic behaviour development during differentiation of ASCs and SPCs.Conclusion:  ASC CM‐derived TGF‐β1‐induced chondrogenic differentiation of ASCs resulted in significant reduction in chondrogenic activity after inhibition of the p38 pathway, revealing involvement of this MAPK pathway in TGF‐β1 signalling. On the other hand, TGF‐β1 signalling also led to SMAD activation that could directly increase chondrogenic activity of ASCs.
Carbamylated erythropoietin increases frataxin independent from the erythropoietin receptor
Authors: Sturm Brigitte , Helminger Melissa , Steinkellner Hannes , Heidari Mohammad Mehdi , Goldenberg Hans , Scheiber‐Mojdehkar Barbara 
Published: April 2010

Eur J Clin Invest 2010; 40 (6): 561–565AbstractBackground  Friedreich’s ataxia (FRDA) is a neurodegenerative disorder caused by decreased expression of the mitochondrial protein frataxin. Recently we showed in a clinical pilot study in Friedreich’s ataxia patients that recombinant human erythropoietin (rhuEPO) significantly increases frataxin‐expression. In this in vitro study, we investigated the role of the erythropoietin receptor (EPO‐R) in the frataxin increasing effect of rhuEPO and if nonerythropoietic carbamylated erythropoietin (CEPO), which cannot bind to the classical EPO‐R increases frataxin expression.Materials and methods  In our experiments human erythroleukaemic K562 cells (+ EPO‐R), human monocytic leukemia THP‐1 cells (− EPO‐R) and isolated primary lymphocytes from healthy control and FRDA patients were incubated with different concentrations of rhuEPO or CEPO. Frataxin‐expression was detected by an electrochemical luminescence immunoassay (based on the principle of an ELISA).Results  We show that rhuEPO increases frataxin‐expression in K562 cells (expressing EPO‐R) as well as in THP‐1 cells (without EPO‐R expression). These results were confirmed by the finding that CEPO, which cannot bind to the classical EPO‐R increased frataxin expression in the same concentration range as rhuEPO. In addition, we show that both EPO derivatives significantly increase frataxin‐expression in vitro in control and Friedreich’s ataxia patients primary lymphocytes.Conclusion  Our results provide a scientific basis for further studies examining the effectiveness of nonerythropoietic derivatives of erythropoietin for the treatment of Friedreich’s ataxia patients.
Temperature sensitivity of suicidal erythrocyte death
Authors: Föller Michael , Braun Manuel , Qadri Syed M. , Lang Elisabeth , Mahmud Hasan , Lang Florian 
Published: April 2010

Eur J Clin Invest 2010; 40 (6): 534–540AbstractBackground  Fever and hyperthermia are frequently associated with anaemia. Under most clinical conditions, they are considered to be two mutually independent clinical consequences of a common cause. The present study explored the possibility that anaemia results from temperature‐sensitive suicidal erythrocyte death or eryptosis. Eryptosis is characterised by cell shrinkage and cell membrane scrambling with phosphatidylserine (PS) exposure at the erythrocyte surface. It is triggered by increase in the cytosolic Ca2+ activity on the one hand and by ceramide formation on the other.Material and methods  Annexin V‐binding was utilised to disclose PS exposure, forward scatter to analyse cell volume, Fluo 3 fluorescence to estimate cytosolic Ca2+ activity, binding of fluorescent antibodies to determine ceramide abundance and a luciferin/luciferase‐based assay to measure the cytosolic ATP concentration.Results  Graded increases in temperature from 37 to 41 °C decreased the forward scatter and stimulated annexin V‐binding of human erythrocytes. The effect was accompanied by increased cytosolic Ca2+ activity, decrease of the cellular ATP content and a moderate rise in ceramide formation. The effect of hyperthermia on annexin V‐binding was significantly blunted by the leukotriene receptor CysLT1 antagonist cinalukast (1 μM).Conclusions  Hyperthermia stimulates Ca2+ entry into erythrocytes leading to cell shrinkage and PS exposure. As PS‐exposing erythrocytes are rapidly cleared from circulating blood, the eryptosis during hyperthermia may cause anaemia.
Mitochondrial genetic diversity and gene flow of common carp from main river drainages in China
Authors: WANG CHENGHUI , LIU HAO , LIU ZHIZHI , WANG JUN , ZOU JIXIN , LI XUEJUN 
Published: April 2010

Summary1. The common carp (Cyprinus carpioL.) is one of the most widely distributed and important freshwater fishes in the world. In China, the common carp has been recognised as three subspecies:C. carpio haematopterus,C. carpio rubrofuscusandC. carpio carpio.The Nanling Mountains have been suggested as providing the dividing line betweenC. carpio haematopterusandC. carpio rubrofuscus. However, the demographic history and gene flow of the common carp in China is not clear.2. We collected mitochondrial COII and D‐loop sequences (1494 bp) from 241 individuals distributed in eight major river drainages across China. The objective was to provide the first investigation into population genetic structure, demographic history and migration patterns of the common carp from these river drainages, and to assess the validity of the three subspecies.3. Phylogenetic analysis did not result in three major monophyletic lineages corresponding to the three subspecies. The Nanling Mountains do not form a border separatingC. carpio haematopterusandC. carpio rubrofuscus.amovashowed low population differentiation, with 11.60% of the molecular variance found among river drainages. PairwiseFSTvalues between river drainages were moderate (0.0331–0.2617). Substantial gene flow detected in coalescent analysis between drainages showed that the Yangtze drainage was the centre from which migrants moved northward, southward and north‐westward. Human‐mediated translocation has confounded our ability to identify subspecies of common carp in China.
Effect of increasing plant maturity in timothy‐dominated grass silage on the performance of growing/finishing Norwegian Red bulls
Authors: Randby Å. T. , Nørgaard P. , Weisbjerg M. R. 
Published: April 2010

AbstractAn investigation was made of the potential for attaining high daily live weight (LW) gain, high feeding efficiency and carcass quality in Norwegian Red (NRF) bulls fed grass silage harvested at early stages of maturity, supplemented with minimal amounts of concentrates. Roundbale silage was produced from timothy‐dominated swards harvested at three stages of maturity: stages 1 and 2 were dominated by tillers in stem elongation with two and three visible nodes, respectively, and stage 3 by tillers at early heading, with visible heads, but without head stems. Crops were wilted rapidly and a formic acid‐based additive applied. All silages were preserved with restricted fermentation. Silage DOMD values were 0·747, 0·708 and 0·647 for harvesting time (H) 1, 2 and 3 respectively. Silages were fed ad libitum as sole feed, or supplemented with 2–4 kg concentrate at increasing LW, to six bulls per treatment from age 7 months (288 kg) to slaughter at 575 kg. Daily LW gain for bulls fed unsupplemented silage was 1423, 1262 and 936 g, respectively, for H1, H2 and H3, and 1570, 1567 and 1357 g for supplemented bulls. For concentrate‐supplemented bulls, higher energy intake increased proportions of carcass fat more for H1 (0·163) than for H2 (0·134) but the same amount of energy was retained in the carcass per MJ net energy growth intake for these two groups. It is concluded that, apart from milk and concentrate fed to calves during the first months, an intensive beef production with NRF bulls finished before 15 months of age may be based entirely on local grass resources.
Expression of classical NF‐κB pathway effectors in human ovarian carcinoma
Authors: Darb‐Esfahani Silvia , Sinn Bruno V , Weichert Wilko , Budczies Jan , Lehmann Annika , Noske Aurelia , Buckendahl Ann‐Christin , Müller Berit Maria , Sehouli Jalid , Koensgen Dominique , Györffy Balazs , Dietel Manfred , Denkert Carsten 
Published: April 2010

Darb‐Esfahani S, Sinn B V, Weichert W, Budczies J, Lehmann A, Noske A, Buckendahl A‐C, Müller B M, Sehouli J, Koensgen D, Györffy B, Dietel M & Denkert C (2010) Histopathology 56. 727–739 Expression of classical NF‐κB pathway effectors in human ovarian carcinomaAims:  Functional studies have demonstrated that nuclear factor (NF)‐κB promotes tumour progression in ovarian cancer cells. However, surprisingly little is known of the expression of effectors of the NF‐κB pathway in human ovarian cancer in vivo.Methods and results:  Immunohistochemistry and in situ hybridization revealed that in a cohort of 85 primary ovarian carcinomas, total p65 expression was inversely correlated to nuclear and cytoplasmic phospho‐IκBα (P = 0.002 and P = 0.05, respectively), and IκBα mRNA expression (P = 0.032). In contrast, phospho‐p65 expression was paralleled by the expression of nuclear (P = 0.027) and cytoplasmic phospho‐IκBα (P = 0.01). Total p65 expression was an adverse prognostic factor for overall survival (P = 0.018). In contrast, total IκBα and phosphorylated nuclear and cytoplasmic IκBα expression were favourable prognostic markers (P = 0.001, P = 0.031, P = 0.001, respectively). Cytoplasmic phospho‐IκBα expression remained a significant prognostic factor on multivariate analysis (P = 0.010). In cultured, stimulated OVCAR‐3 ovarian cancer cells the cytoplasmic retranslocation of p65 was delayed by inhibition of the nuclear membrane transporter chromosomal region maintenance/exportin1 protein (CRM1). A positive association of p65 and CRM1 expression was demonstrated in ovarian cancer tissue (P < 0.0001).Conclusions:  Total and phosphorylated IκBα protein expression might serve as markers for NF‐κB activation in human ovarian carcinoma. Cytoplasmic localization of p65 may be related to deregulated nucleocytoplasmic transport in carcinomas overexpressing CRM1.
Reproducibility in the diagnosis of needle core biopsies of non‐palpable breast lesions: an international study using virtual slides published on the world‐wide web
Authors: Zito Francesco Alfredo , Verderio Paolo , Simone Giovanni , Angione Vito , Apicella Paola , Bianchi Simonetta , Conde Antonio Felix , Hameed Omar , Ibarra Julio , Leong Antony , Pennelli Natale , Pezzica Ezio , Vezzosi Vania , Ventrella Vincenzo , Pizzamiglio Sara , Paradiso Angelo , Ellis Ian 
Published: April 2010

Zito F A, Verderio P, Simone G, Angione V, Apicella P, Bianchi S, Conde A F, Hameed O, Ibarra J, Leong A, Pennelli N, Pezzica E, Vezzosi V, Ventrella V, Pizzamiglio S, Paradiso A & Ellis I (2010) Histopathology 56, 720–726 Reproducibility in the diagnosis of needle core biopsies of non‐palpable breast lesions: an international study using virtual slides published on the world‐wide webAims:  To conduct an internet‐based study using virtual slides (VS) of sterotactic core biopsy specimens of non‐palpable breast lesions in order to evaluate interobserver reproducibility between pathologists.Methods and results:  A total of 18 breast lesions, determined to be histologically complex by two pathologists, were selected. Digitized VSs were then created using QuickTime Virtual Reality technology (Apple, Cupertino, CA, USA) and posted on the world‐wide web. In all, 10 pathologists completed the evaluations of 18 VSs using the five diagnostic categories (B1–B5) from the European guidelines for quality assurance in breast cancer screening and diagnosis. Their results were compared with those of every other participating pathologist, and were then individually compared with the results of a highly experienced breast pathologist (referee). Of the 18 cases, 10 (56%) were classified by the referee as borderline (B3 and B4). Comparisons with reference values showed a less than satisfactory level of reproducibility (median κw = 0.60). As regards interobserver reproducibility, results showed that, in general, the level of agreement was not satisfactory (median κw = 0.53).Conclusions:  Overall, the findings are comparable to those quality control studies using circulating slides when analysis is done on borderline cases.
Cumulative alterations of p27Kip1‐related cell‐cycle regulators in the development of endometriosis‐associated ovarian clear cell adenocarcinoma
Authors: Yamamoto Sohei , Tsuda Hitoshi , Miyai Kosuke , Takano Masashi , Tamai Seiichi , Matsubara Osamu 
Published: April 2010

Yamamoto S, Tsuda H, Miyai K, Takano M, Tamai S & Matsubara O (2010) Histopathology 56, 740–749 Cumulative alterations of p27Kip1‐related cell‐cycle regulators in the development of endometriosis‐associated ovarian clear cell adenocarcinomaAims:  To identify the key cell‐cycle dysregulations in the development of endometriosis‐associated ovarian clear cell adenocarcinoma (CCA).Methods and results:  Expression of p27Kip1‐interacting cell‐cycle regulators, such as p27Kip1 itself, Skp2, cyclin‐dependent kinase subunit 1 (Cks1), cyclin A and cyclin E, and Ki67 labelling index (LI), were analysed by immunohistochemistry in 23 CCAs with 36 endometriotic or atypical endometriotic lesions adjacent to CCA from a cohort of 23 patients, and in 31 cases of solitary endometriosis. The cell‐cycle regulators examined were overexpressed (Skp2, Cks1, cyclin A and cyclin E; P < 0.01, each) or down‐regulated (p27Kip1, P = 0.044) significantly more frequently in the CCAs than in the adjacent endometriosis. The frequency of Skp2 overexpression was significantly higher in atypical endometriosis than in endometriosis, and the frequency of Skp2 and cyclin A overexpression was significantly higher in CCA than in atypical endometriosis (P < 0.01, each). Mean Ki67 LI increased from endometriosis (8.4%) through atypical endometriosis (21.4%) to CCA (46.9%), with statistical significance between each component (P < 0.01, each). The frequency of cell‐cycle regulator expression and mean Ki67 LIs were not significantly different between solitary endometriosis and endometriosis adjacent to CCA.Conclusions:  Alteration of the p27Kip1‐interacting cell‐cycle regulators appeared strongly involved in the progression of endometriosis‐associated ovarian clear cell carcinogenesis through increasing cell proliferative activity.
Glypican 3 has a higher sensitivity than alpha‐fetoprotein for testicular and ovarian yolk sac tumour: immunohistochemical investigation with analysis of histological growth patterns
Authors: Zynger Debra L , McCallum John C , Luan Chunyan , Chou Pauline M , Yang Ximing J 
Published: April 2010

Zynger D L, McCallum J C, Luan C, Chou P M & Yang X J (2010) Histopathology 56, 750–757 Glypican 3 has a higher sensitivity than alpha‐fetoprotein for testicular and ovarian yolk sac tumour: immunohistochemical investigation with analysis of histological growth patternsAims:  Glypican 3 (GPC3) has been reported to be overexpressed in yolk sac tumour (YST), but the sensitivity has not been compared with α‐fetoprotein (AFP). YST can form numerous growth patterns and the expression of GPC3 in these patterns has not been studied. The aim was to address these aspects.Methods and results:  Sections from testicular or ovarian YST were subjected to immunohistochemistry using GPC3 (n = 39) and AFP (n = 24). Overall immunoreactivity for each case and specific histological patterns were semiquantitatively evaluated (0–3+) and intensity of reactivity was scored (0–3). All cases expressed GPC3 (1+, 5%; 2+, 8%; 3+, 87%) with strong intensity (2.9). The majority expressed AFP (58%) but immunoreactivity was often focal (0, 42%; 1+, 33%; 2+, 25%) and intensity was low (1.0). Using GPC3, >75% of the microcystic (n = 38), macrocystic (n = 26), solid (n = 21), glandular‐alveolar (n = 8), endodermal sinus (n = 7), polyvesicular vitelline (n = 5), enteric (n = 4) and micropapillary (n = 2) growth patterns displayed 2+ or 3+ positivity.Conclusions:  YST can display a variety of growth patterns that can be confused with other germ cell tumour components. GPC3 detects all growth patterns tested and has a higher sensitivity for detecting YST than AFP.
Breeding tef [Eragrostis tef (Zucc.) trotter]: conventional and molecular approaches
Authors: Assefa K. , Yu J.‐K. , Zeid M. , Belay G. , Tefera H. , Sorrells M. E. 
Published: April 2010

With 2 figures and 5 tables AbstractTef [Eragrostis tef (Zucc.) Trotter] is an important food staple cereal crop in Ethiopia. Despite its importance in the Ethiopian agriculture, there are constraints that need to be addressed through scientific research. The major constraints are low yield of landrace cultivars under widespread cultivation, susceptibility to lodging and a lack of knowledge concerning the genetic control of agronomic traits. Conventional tef breeding efforts started in the late 1950s, and since then a total of 24 varieties have been developed and released. Yield gain from tef breeding has been linear with an average annual increase of 0.8%. Tef genomics has provided much molecular genetic information on important agronomic traits. More than 1500 PCR‐based molecular markers have been developed and several genetic linkage maps based on intra‐ and inter‐specific crosses have been constructed. Results from quantitative trait loci studies have provided information necessary for marker‐assisted selection. Lodging is the number one cause of yield loss in tef. Recently, molecular breeding techniques and biotechnologies are being employed to understand the genetic control of lodging.
Long‐term follow up of autologous serum treatment for recurrent corneal erosions
Authors: Ziakas Nikolaos G , Boboridis Konstantinos G , Terzidou Chryssa , Naoumidi Tatiana L , Mikropoulos Dimitris , Georgiadou Eirini N , Georgiadis Nick S 
Published: April 2010

AbstractPurpose:  The aim of the study was to evaluate long‐term results of autologous serum treatment for recurrent corneal erosions.Methods:  In this prospective single‐centre study, 33 eyes of 33 patients (21 male and 12 female) were treated with autologous serum eye drops for recurrent corneal erosions. Mean age of the patients was 49.3 ± 9.8 standard deviation (range 24–73) years. All subjects had failed to respond to other treatments. Autologous serum drops were administered for a 6‐month period: six times daily for the first 3 months and four times daily for the remaining 3 months. Detailed informed consent was obtained from the entire patient group before the study.Results:  The mean follow‐up period was 30 ± 6.3 standard deviation (range 12–48) months. None of the patients experienced a recurrence while under treatment. Twenty‐eight patients (85%) had complete healing of erosions with no relapses of the disease over the whole follow‐up period. Five patients (15%) presented a single recurrence 3–12 months after the end of the treatment. No sight‐threatening complications were reported over the follow up. There was no statistically significant difference in the best spectacle‐corrected visual acuity values (tstat = 2.1, F = 0.096, degree of freedom = 40 166, P < 0.41) or in the intraocular pressure measurements (P < 0.38) between the pre‐ and post‐treatment patient groups.Conclusions:  Autologous serum drops proved to be a safe and efficient treatment modality for patients with recurrent corneal erosion syndrome as observed through a long‐term follow up.