bluuebunny/crossref_metadata_2025_split huggingface dataset:
Celecoxib enhanced the sensitivity of cancer cells to anticancer drugs by inhibition of the expression of P‐glycoprotein through a COX‐2‐Independent Manner
Authors: Xia Wenhong , Zhao Tao , Lv Jinghuan , Xu Shan , Shi Junfeng , Wang Shui , Han Xiao , Sun Yujie 
Published: June 2009

AbstractThe P‐glycoprotein (p170, P‐gp) encoded by humanMDR1gene functions as a pump to extrude anticancer drugs from cancer cells. Over‐expression of p170 is closely related to primary and induced drug resistance phenotype of tumor cells. Recent studies have demonstrated that expression of cyclooxygenase‐2 (COX‐2) is positively correlated with the p170 level, suggesting a potential of COX‐2 specific inhibitors in regulation of cytotoxicity of anticancer agents. Celecoxib is one of the specific inhibitors of COX‐2 and has been widely used in clinic. However, its function in the response of cancer cells to anticancer drugs and the related mechanism are still waiting to be investigated. To explore the correlation of celecoxib and the p170‐mediated drug resistance, the role of celecoxib in drug response of cancer cells was analyzed with flow cytometry, high performance liquid chromatography (HPLC), and colony formation experiments. Celecoxib (50 µM) was found to significantly enhance the sensitivity of MCF‐7 and JAR/VP16 cells to tamoxifen and etoposide, respectively, by inhibition of p170 expression and increase in intracellular accumulation of the drugs. However, celecoxib did not affect pump function of p170. Enzyme activity and methylation analyses demonstrated that the inhibitory effect of celecoxib on p170 was independent on COX‐2 but closely related to hypermethylation ofMDR1gene promoter. Our study suggested that celecoxib was a potential agent for enhancement of the sensitivity of cancer cells to anticancer drugs. It also provided a links between epigenetic change ofMDR1and drug response of cancer cells. J. Cell. Biochem. 108: 181–194, 2009. © 2009 Wiley‐Liss, Inc.
SIRT1: A novel target to prevent atherosclerosis
Authors: Yu Wei , Fu Yu‐Cai , Chen Chun‐Juan , Wang Xin , Wang Wei 
Published: June 2009

AbstractAtherosclerosis is a chronic immuno‐inflammatory disease associated with blood lipids disorder. Many studies have demonstrated that caloric restriction (CR) can prevent atherosclerosis and extend lifespan. Sir2 protein, mammal's SIRT1, has been reported to at least partly contribute to the protective effect of CR. Hence, we hypothesize that SIRT1 is a key regulator in the pathogenesis of atherosclerosis and that upregulation of SIRT1 in endothelial cells may mimic CR's beneficial effect on vascular health. The recent studies have demonstrated that endothelial SIRT1 is an anti‐atherosclerosis factor and the possible mechanism may be related to inhibit oxidized low‐density lipoprotein (oxLDL)‐induced apoptosis, upregulate endothelial nitric oxide synthase (eNOS) expression, and improve endothelium relaxation function. We infer that SIRT1 may be a novel target for atherosclerosis prevention and treatment. J. Cell. Biochem. 108: 10–13, 2009. © 2009 Wiley‐Liss, Inc.
RUNX genes find a niche in stem cell biology
Authors: Appleford Peter J. , Woollard Alison 
Published: June 2009

AbstractThe RUNX family of transcriptional regulators are well conserved throughout the animal kingdom, from the simple nematode worm Caenorhabditis elegans to vertebrates. Interest in the RUNX genes emerged principally as a result of the finding that chromosomal translocations disrupting RUNX protein function are observed in a large number of patients suffering with acute myeloid leukemia (AML). In the 20 years that RUNX genes have been under investigation, they have emerged as central players in the control of developmental decisions between proliferation and differentiation in a wide variety of biological situations. This review focuses on recent data highlighting the roles of RUNX genes in stem cells and illustrates the diversity of processes in which the RUNX proteins play a critical role. In particular, we focus on the role of RUNX1 in hematopoietic stem cells (HSCs) and hair follicle stem cells (HFSCs) and the importance of the solo C. elegans RUNX factor rnt‐1 in stem cell proliferation in the worm. Observations in a variety of stem cell systems have developed to the point where useful comparisons can be made, from which guiding principles may emerge. J. Cell. Biochem. 108: 14–21, 2009. © 2009 Wiley‐Liss, Inc.
Thyroid hormones induce cell proliferation and survival in ovarian granulosa cells COV434
Authors: Verga Falzacappa Cecilia , Mangialardo Claudia , Patriarca Valentina , Bucci Barbara , Amendola Donatella , Raffa Salvatore , Torrisi Maria Rosaria , Silvestrini Giuliana , Ballanti Paola , Moriggi Giulia , Stigliano Antonio , Brunetti Ercole , Toscano Vincenzo , Misiti Silvia 
Published: June 2009

AbstractNumerous evidences indicate that thyroid hormones exert an important role in the regulation of the reproductive system in the adult female. Although a clear demonstration of the thyroid–ovarian interaction is still lacking, it is conceivable that thyroid hormones might have a direct role in ovarian physiology via receptors in granulosa cells. In this study we analyzed if thyroid hormone treatment could affect cell proliferation and survival of COV434 cells. To this aim cell growth experiments and cell cycle analyses by flow cytometry were performed. Secondly the T3 survival action was tested by TUNEL assay and MD30 cleavage analysis. We showed that T3, and not T4, can protect ovarian granulosa cells COV434 from apoptosis, regulating cell cycle and growth in the same cells. The increase in cell growth resulted in an augmented percentage of the cells in the S phase and, in a reduction of the doubling time (18%). Subsequently apoptotic pathway induced by serum deprivation has been evaluated in the cells exposed or not to thyroid hormone treatment. The T3 treatment was able to remarkably counteract the apoptotic process. Even at the ultrastructural level there was an evident protective effect of T3 in the cells that, besides the maintenance of the original morphology and, the absence of basophilic cytoplasm, conserved normal junctional areas. Furthermore, the protective T3 effect evaluated by FACS analysis in the presence of a PI3K inhibitor revealed, as also confirmed by Western Blot on pAkt, that the PI3K pathway is crucial in T3 survival action. J. Cell. Physiol. 221: 242–253, 2009. © 2009 Wiley‐Liss, Inc
Orderly hematopoietic development of induced pluripotent stem cells via Flk‐1+ hemoangiogenic progenitors
Authors: Niwa Akira , Umeda Katsutsugu , Chang Hsi , Saito Megumu , Okita Keisuke , Takahashi Kazutoshi , Nakagawa Masato , Yamanaka Shinya , Nakahata Tatsutoshi , Heike Toshio 
Published: June 2009

AbstractInduced pluripotent stem (iPS) cells, reprogrammed somatic cells with embryonic stem (ES) cell‐like characteristics, are generated by the introduction of combinations of specific transcription factors. Little is known about the differentiation of iPS cells in vitro. Here we demonstrate that murine iPS cells produce various hematopoietic cell lineages when incubated on a layer of OP9 stromal cells. During this differentiation, iPS cells went through an intermediate stage consisting of progenitor cells that were positive for the early mesodermal marker Flk‐1 and for the sequential expression of other genes that are associated with hematopoietic and endothelial development. Flk‐1+ cells differentiated into primitive and definitive hematopoietic cells, as well as into endothelial cells. Furthermore, Flk‐1+ populations contained common bilineage progenitors that could generate both hematopoietic and endothelial lineages from single cells. Our results demonstrate that iPS cell‐derived cells, like ES cells, can follow a similar hematopoietic route to that seen in normal embryogenesis. This finding highlights the potential use of iPS cells in clinical areas such as regenerative medicine, disease investigation, and drug screening. J. Cell. Physiol. 221: 367–377, 2009. © 2009 Wiley‐Liss, Inc.
Stem cell pluripotency: A cellular trait that depends on transcription factors, chromatin state and a checkpoint deficient cell cycle
Authors: Boheler Kenneth R. 
Published: June 2009

AbstractEmbryonic stem (ES) and induced pluripotent stem (iPS) cells self‐renew and are pluripotent. Differentiation of these cells can yield over 200 somatic cell types, making pluripotent cells an obvious source for regenerative medicine. Before the potential of these cells can be maximally harnessed for clinical applications, it will be necessary to understand the processes that maintain pluripotentiality and signal differentiation. Currently, three unique molecular properties distinguish pluripotent stem cells from somatic cells. These include a unique transcriptional hierarchy that sustains the pluripotent state during the process of self‐renewal; a poised epigenetic state that maintains chromatin in a form ready for rapid cell fate decisions; and a cell cycle characterized by an extremely short gap 1 (G1) phase and the near absence of normal somatic cell checkpoint controls. Recently, B‐MYB (MYBL2) was implicated in the gene regulation of two pluripotency factors and normal cell cycle progression. In this article, the three pluripotency properties and the potential role of B‐Myb to regulate these processes will be discussed. J. Cell. Physiol. 221: 10–17, 2009. © 2009 Wiley‐Liss, Inc
A convenient approach to the canthin‐4‐one ring system: Total synthesis of the alkaloids tuboflavine and norisotuboflavine
Authors: Puzik Andreas , Bracher Franz 
Published: June 2009

Abstract
magnified imageCanthin‐4‐one, as well as 5‐ and 6‐alkylcanthin‐4‐ones are readily available through reaction of 1‐acyl‐β‐carbolines with Bredereck's reagent or dimethylacetamide acetals in anhydrous DMF. The intermediate enaminoketones readily undergo cyclization to the canthin‐4‐ones. The alkaloids tuboflavine and norisotuboflavine were prepared following this methodology. J. Heterocyclic Chem., (2009).
Seasonality and spatial pattern of rainfall of Sri Lanka: Exploratory factor analysis
Authors: Wickramagamage P. 
Published: June 2009

AbstractThis analysis is based on monthly means of rainfall at a dense network of gauging stations in Sri Lanka. The mean monthly values of rainfall at 646 stations were used as variables to characterise the individual stations. These variables show a significant correlation among most of them. The highest correlations were found between months within the same meteorological season, with one exception. The exception is that of October which has a higher correlation with months of southwest monsoon (SWM) than with the inter‐monsoon (IM) months. The IM months and November have moderate values of correlation with the months of SWM. All three months of northeast monsoon (NEM) are strongly correlated and form a clearly defined group. This pattern of correlation can be explained in terms of the spatial distribution of rainfall of the 12 months. The strongly correlated months have a similar spatial pattern. This indicates that the number of distinct spatial modes of rainfall is less than 12. To discover these modes, principal component analysis (PCA) and factor analysis (FA) were applied on the data set. Of the two ordination methods, FA produced more easily interpretable results than PCA. The factor solution identified four spatiotemporal rainfall modes—weak southwest (SW) mode (March–April), strong SW mode (May–October), strong NEM mode (December–February) and mixed mode (November). These modes have strong similarity to the monthly rainfall surfaces created using the original data of the same periods. Copyright © 2009 Royal Meteorological Society
Are current recommendations to diagnose orthostatic hypotension in Parkinson's disease satisfactory?
Authors: Jamnadas‐Khoda Jenny , Koshy Suma , Mathias Christopher J. , Muthane Uday B. , Ragothaman Mona , Dodaballapur Subbakrishna K. 
Published: June 2009

AbstractWe interviewed 50 Parkinson's disease (PD) patients using a questionnaire to verify the reliability of orthostatic symptoms in warning the presence of orthostatic hypotension (OH). OH is defined as 20 mm Hg systolic or 10 mm Hg diastolic BP fall within 3 min of tilting or standing but if this fall occurs after 3 min we called it ‘late OH’ (L‐OH). We compared if OH in Parkinson's disease (PD) was more frequent after head‐up tilt or on standing and if the period of postural challenge matters in detecting OH. Twenty‐one (42%) patients had OH that occurred twice more often after tilting (n = 20) than on standing (n = 10). OH occurred within 3 min of tilting in 9 patients (18%) and appeared beyond the currently recommended 3 min in 11 patients (55%) (L‐OH). Ten of the 20 patients developing OH on tilting were symptomatic. The 10 patients who had OH on standing were asymptomatic. Reporting of symptoms was independent of age or severity of BP fall. Most (90%) patients reporting orthostatic symptoms on standing had OH on tilting for 3 min. Orthostatic symptoms in PD have a high specificity but low sensitivity in predicting OH. In Parkinson's disease OH occurs often after tilting than on standing and is delayed (after 3 min). As OH in PD is often asymptomatic and delayed it could contribute to falls and increase morbidity. We suggest routine evaluation of OH in PD by tilting them longer than the recommended 3 minutes to detect delayed OH. © 2009 Movement Disorder Society
Paraneoplastic movement disorders
Authors: Grant Robin , Graus Francesc 
Published: June 2009

AbstractParaneoplastic movement disorders are rare autoimmune nonmetastatic complications of cancer. Common paraneoplastic movement disorders include cerebellar syndrome, opsoclonus myoclonus, basal ganglia disorders, stiff person syndrome, and neuromyotonia. Syndromes usually present before cancer diagnosis and are commonly associated with one or more serum antibodies. Increasing numbers of antibodies have been identified (Hu, Yo, Ri, CV2, amphiphysin, Ma, Ta, Tr, NMDA, mGluR1, PCA2, ANNA‐3, VGCCA). Antibodies are highly correlated with the likelihood of an underlying cancer and are closely associated with certain tumors. Clinical clues to paraneoplastic aetiology include speed of onset, severity, speed of progression, resistance to treatment, and more widespread neurological signs than one would expect from nonparaneoplastic aetiologies. Cancer should be sought in those with classical presentations and those with possible presentations who have paraneoplastic antibodies. If no tumor is found on initial investigation, interval screening is advisable. The most common associated cancers found are small cell lung cancer, breast, gynaecological, testicular, lymphoma, and thymoma. Early identification and treatment sometimes leads to neurological improvement and may improve cancer prognosis. Prognosis is dependent on the tumor type and its likely response to treatment. © 2009 Movement Disorder Society
Association of DRD3 and GRIN2B with impulse control and related behaviors in Parkinson's disease
Authors: Lee Jee‐Young , Lee Eun Kyung , Park Sung Sup , Lim Ji‐Yeon , Kim Hee Jin , Kim Ji Sun , Jeon Beom S 
Published: June 2009

AbstractWe aimed to assess whether allelic variants of dopamine receptor, glutamate receptor, and serotonin transporter genes are associated with the appearance of impulse control and related behaviors (ICRB) in Parkinson's disease (PD) with dopamine replacement therapy (DRT). We surveyed ICRB in consecutive Korean patients with PD who were treated with stable DRT using modified Minnesota Impulsive Disorders Interview over a period of 4 months. In the 404 patients who completed the interview and the 559 Korean healthy normal controls, genotyping was performed for variants of the DRD3 p.S9G, DRD2 Taq1A, GRIN2B c.366C>G, c.2664C>T and c.‐200T>G, and the promoter region of the serotonin transporter gene (5‐HTTLPR). Behavioral abnormalities suggestive of ICRB including compulsive buying, gambling, sexual behavior and eating, and punding, were present in 14.4% of the patients. Variants of DRD2 and 5‐HTTLPR were not associated with the risk of developing ICRB. However, the AA genotype of DRD3 p.S9G and the CC genotype of GRIN2B c.366C>G were more frequent in patients with ICRB than in nonaffected patients (odds ratio [OR] = 2.21, P = 0.0094; and 2.14, P = 0.0087, after adjusting for age and sex). After controlling for clinical variables in the multivariate analysis, carriage of either AA genotype of DRD3 or CC genotype of GRIN2B was identified as an independent risk factor for ICRB (adjusted OR: 2.57, P = 0.0087). Variants of DRD3 p.S9G and GRIN2B c.366C>G may be associated with the appearance of ICRB in PD. © 2009 Movement Disorder Society
Development of a brief ataxia rating scale (BARS) based on a modified form of the ICARS
Authors: Schmahmann Jeremy D. , Gardner Raquel , MacMore Jason , Vangel Mark G. 
Published: June 2009

AbstractTo develop a brief ataxia rating scale (BARS) for use by movement disorder specialists and general neurologists. Current ataxia rating scales are cumbersome and not designed for clinical practice. We first modified the International Cooperative Ataxia Rating Scale (ICARS) by adding seven ataxia tests (modified ICARS, or MICARS), and observed only minimally increased scores. We then used the statistics package R to find a five‐test subset in MICARS that would correlate best with the total MICARS score. This was accomplished first without constraints and then with the clinical constraint requiring one test each of Gait, Kinetic Function‐Arm, Kinetic Function‐Leg, Speech, and Eye Movements. We validated these clinical constraints by factor analysis. We then validated the results in a second cohort of patients; evaluated inter‐rater reliability in a third cohort; and used the same data set to compare BARS with the Scale for the Assessment and Rating of Ataxia (SARA). Correlation of ICARS with the seven additional tests that when added to ICARS form MICARS was 0.88. There were 31,481 five‐test subtests (48% of possible combinations) that had a correlation with total MICARS score of ≥0.90. The strongest correlation of an unconstrained five‐test subset was 0.963. The clinically constrained subtest validated by factor analysis, BARS, had a correlation with MICARS‐minus‐BARS of 0.952. Cronbach alpha for BARS and SARA was 0.90 and 0.92 respectively; and inter‐rater reliability (intraclass correlation coefficient) was 0.91 and 0.93 respectively. BARS is valid, reliable, and sufficiently fast and accurate for clinical purposes. © 2009 Movement Disorder Society
Asymptotic approximation of eigenelements of the Dirichlet problem for the Laplacian in a domain with shoots
Authors: Amirat Youcef , Chechkin Gregory A. , Gadyl'shin Rustem R. 
Published: June 2009

AbstractWe study the asymptotic behavior of the eigenelements of the Dirichlet problem for the Laplacian in a two‐dimensional bounded domain with thin shoots, depending on a small parameter ε. Under the assumption that the width of the shoots goes to zero, as ε tends to zero, we construct the limit (homogenized) problem and prove the convergence of the eigenvalues and eigenfunctions to the eigenvalues and eigenfunctions of the limit problem, respectively. Under the additional assumption that the shoots, in a fixed vicinity of the basis, are straight and periodic, and their width and the distance between the neighboring shoots are of order ε, we construct the two‐term asymptotics of the eigenvalues of the problem, as ε→0. Copyright © 2009 John Wiley & Sons, Ltd.
Non‐invasive determination of tissue thermal parameters from high intensity focused ultrasound treatment monitored by volumetric MRI thermometry
Authors: Dragonu Iulius , de Oliveira Philippe Lourenço , Laurent Christophe , Mougenot Charles , Grenier Nicolas , Moonen Chrit T. W. , Quesson Bruno 
Published: June 2009

AbstractA method is proposed for estimating the perfusion rate, thermal diffusivity, and the absorption coefficient that influence the local temperature during high intensity focused ultrasound (HIFU) thermotherapy procedures. For this purpose, HIFU heating experiments (N = 100) were performed ex vivo on perfused porcine kidney (N = 5) under different flow conditions. The resulting spatio‐temporal temperature variations were measured non‐invasively by rapid volumetric MR‐temperature imaging. The bio‐heat transfer (BHT) model was adapted to describe the spatio‐temporal evolution of tissue temperature in the cortex. Absorption and perfusion coefficients were determined by fitting the integrated thermal load (spatial integration of the thermal maps) curves in time with an analytical solution of the BHT equation proposed for single point HIFU heating. Thermal diffusivity was determined independently by analyzing the spatial spread of the temperature in time during the cooling period. Absorption coefficient and thermal diffusivity were found to be independent of flow, with mean and average values of 11.0 ± 1.85 mm3 · K · J−1 and 0.172 ± 0.003 mm2 · s−1, respectively. A linear dependence of the calculated perfusion rate with flow was observed with a slope of 9.20 ± 0.75 mm−3. The perfusion was found to act as a scaling term with respect to temperature but with no effect on the spatial spread of temperature which only depends on the thermal diffusivity. All results were in excellent agreement with the BHT model, indicating that this model is suitable to predict the evolution of temperature in perfused organs. This quantitative approach allows for determination of tissue thermal parameters with excellent precision (within 10%) and may thus help in quantifying the influence of perfusion during MR guided high intensity focused ultrasound (MRgHIFU). Copyright © 2009 John Wiley & Sons, Ltd.
A new analytical method for system of ODEs
Authors: Aminikhah Hossein , Biazar Jafar 
Published: June 2009

AbstractIn this article, a new homotopy perturbation method (NHPM) is introduced to obtain exact solutions of system of ODEs. Theoretical considerations are discussed. Comparison of the results of applying NHPM with those of homotopy perturbation method (HPM) and Adomia's decomposition method (ADM) leads to significant consequences. Three examples, including stiff system of linear and nonlinear ODEs are given to demonstrate the efficiency of the new method. © 2009 Wiley Periodicals, Inc. Numer Methods Partial Differential Eq 2010
Synthesis of thermo‐responsive microgels in supercritical carbon dioxide using ethylene glycol dimethacrylate as a cross‐linker
Authors: Hu Yaodong , Cao Liqin , Xiao Feng , Wang Jide 
Published: June 2009

AbstractHerein, we report the preparation of thermo‐responsive polymers in a green medium. The white, dry, fine powders were obtained directly from the cross‐linking polymerization of N‐isopropylacrylamide (NIPA) in supercritical carbon dioxide (scCO2) at pressures ranging from 10 to 28 MPa utilizing ethylene glycol dimethacrylate (EGDMA) as a cross‐linker. The effects of reaction pressure, cross‐linker ratio, initiator concentration, and reaction time were investigated. In the presence of this cross‐linker (26.4% w/w), much smaller poly(N‐isopropylacrylamide) (PNIPA) microgels (<0.2 µm diameter) were formed, and it was shown that the particle size and the morphology of the polymer were strongly dependent on the cross‐linker ratio in scCO2. Copyright © 2009 John Wiley & Sons, Ltd.