bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comparative analysis of primer sets for the assessment of clonality in feline lymphomas
Authors: Weyrich Angelika , Hecht Werner , Köhler Kernt , Herden Christiane , Henrich Manfred 
Published: May 2024

IntroductionLymphomas are among the most important and common malignant tumors in cats. Differentiating lymphomas from reactive lymphoid proliferations can be challenging, so additional tools such as clonality assessment by PCR are important in diagnosis finding. Several PCR assays have been developed to assess clonality in feline lymphomas. For T-cell lymphomas TRG (T-cell receptor gamma) genes are the preferred target whereas for B-cell lymphomas most primer sets target immunoglobulin heavy chain (IGH) genes. Here we compare commonly used diagnostic primer sets for the assessment of clonality in feline lymphomas under controlled conditions (i.e., identical sample set, PCR setup, amplicon detection system).MethodsFormalin-fixed and paraffin-embedded samples from 31 feline T-cell lymphomas, 29 B-cell lymphomas, and 11 non-neoplastic controls were analyzed by PCR combined with capillary electrophoresis.Results and discussionWe show that the combination of the primer sets published by Weiss et al. and Mochizuki et al. provided the best results for T-cell clonality, i.e., correctly assigns most populations as clonal or polyclonal. For B-cell clonality, the combination of the primer sets by Mochizuki et al. and Rout et al. gave the best results when omitting the Kde gene rearrangement due to its low specificity. This study rigorously evaluated various primer sets under uniform experimental conditions to improve accuracy of lymphoma diagnostic and provides a recommendation for achieving the highest diagnostic precision in lymphoma clonality analysis.
Penyuluhan Perilaku Hidup Bersih Sehat Dan Pemberian Bantuan Sabun Cuci Tangan (Handsanitizer) Serta Pemeriksaan Hemoglobin Pada Murid Madrasah Ibtidaiyah Swasta Sholihin Tanjung Morawa Deli Serdang
Authors: M.F. Situmeang Suryani , Setiyawati Dewi , Suparni Suparni 
Published: May 2024

Clean and Healthy Living Behavior is a reflection of a family lifestyle that always pays attention to and maintains the health of all family members. School-aged children are a critical age group, because at that age a child is vulnerable to health problems. Based on our observations, students at the Solihin Tanjung Morawa Private Madrasah Ibtidaiyah carry out activities during breaks at school, they snack in the canteen around the school without washing their hands first due to a lack of knowledge and limited running water facilities. As an alternative for running water facilities, you can use hand sanitizer. Although the best method is to wash your hands with soap and running water. The Clean and Healthy Living Behavior Extension aims to increase the knowledge and understanding of students at the Solihin Tanjung Morawa Private Madrasah Ibtidaiyah School about clean and healthy living behavior, which will ultimately improve the level of children's health. Sometimes washing your hands becomes difficult because it is not always easy to find water and soap. As an alternative, you can use a hand sanitizer, an antiseptic liquid or gel that can be used anywhere and at any time without having to rinse with water. After carrying out outreach activities at the Sholihin Private Madrasah Ibtidaiyah School on July 25 2023, it can be concluded that: There has been an increase in the knowledge of students at the Sholihin Private Madrasah Ibtidaiyah School regarding clean living and how to wash their hands using hand sanitizer correctly. The condition of the Hemoglobin Levels of students at the Sholihin Private Madrasah Ibtidaiyah School whose scores were below normal was 3% of the 60 students examined.
KONDISI PADANG LAMUN DI SEKITAR PERAIRAN DESA BOWONGKALI KECAMATAN TABUKAN TENGAH KABUPATEN KEPULAUAN SANGIHE
Authors: Darui Dedyes Alescandro , Sondak Calvyn F. A. , Warouw Veibe , Kemer Kurniati , Gerung Grevo Soleman , Wagey Billy Theodorus 
Published: May 2024

Seagrass is a flowering plant (Angiospermae) that can live in the sea. Seagrass ecosystems have a very important role both ecologically and biologically in coastal and estuary areas. These plants provide food for many marine animals include herbivorous invertebrates, and herbivorous fish. This study aims to determine the condition of seagrass beds in the waters of Bowongkali Village, Central Tabukan District, Sangihe Islands Regency based on the percentage cover. Data collection method in this study uses the quadratic transect method with three transect lines with a length of 100m each and a distance between each transect is 50m. Data was collected by determining the percentage of seagrass cover and seagrass species composition in each small box in the quadrat frame on each transect. The results of this study obtained the total average percentage has a percentage value of seagrass cover of 48.67% and categorized as "medium". The types of seagrasses found are Enhalus acoroides, Thalassia hemprichii, Cymodocea rotundata, Syringodium isoetifolium, Halophila ovalis. Envoronmental parameters recorded temperature range 31.5-32 ° C, salinity range 26-28‰ and pH 8.02-8.07.
Keywords: Seagrass beds, Condition, Waters of Bowongkali Village.

ABSTRAK
Lamun adalah tumbuhan berbunga (Angiospermae) yang dapat hidup di laut. Ekosistem padang lamun memiliki peran sangat penting baik secara ekologi maupun biologi di kawasan pesisir dan estuari. Tumbuhan ini berperan sebagai produsen dan menyediakan makanan bagi invertebrata herbivora, dan ikan herbivora. Penelitian ini bertujuan untuk mengetahui keanekaragaman jenis lamun dan kondisi padang lamun di Perairan Desa Bowongkali, Kecamatan Tabukan Tengah, Kabupaten Kepulauan Sangihe. Pengambilan Data pada penelitian ini mengunakan metode transek kuadrat dengan tiga line transek yang memiliki panjang masing-masing 100m dengan jarak antar tiap transek adalah 50m (100 x 100 m²). Pengambilan data dilakukan dengan cara mentukan nilai persentase tutupan lamun dan komposisi jenis lamun pada setiap kotak kecil dalam frame kuadrat pada masing-masing transek. Hasil penelitian ini didapatkan, nilai persentase tutupan padang lamun sebesar 48,67% termasuk dalam kategori “sedang”. Jenis-jenis lamun yang ditemukan yaitu, Enhalus acoroides, Thalassia hemprichii, Cymodocea rotundata, Syringodium isoetifolium, Halophila ovalis. Parameter di Perairan Desa Bowongkali yaitu suhu, salinitas, pH, nilai suhu 31,5-32°C, nilai salinitas 26-28‰ dan nilai pH 8,02-8,07.
Kata Kunci: Padang lamun, Kondisi, Perairan Desa Bowongkali
Towards sub-10 nm spatial resolution by tender X-ray ptychographic coherent diffraction imaging
Authors: Ishiguro Nozomu , Kaneko Fusae , Abe Masaki , Takayama Yuki , Yoshida Junya , Hoshino Taiki , Takazawa Shuntaro , Uematsu Hideshi , Sasaki Yuhei , Okawa Naru , Takahashi Keichi , Takizawa Hiroyuki , Kishimoto Hiroyuki , Takahashi Yukio 
Published: May 2024

Abstract
As the first experiment at BL10U in NanoTerasu, tender X-ray ptychographic coherent diffraction imaging (PCDI) was conducted using a photon energy of 3.5 keV. The ptychographic diffraction patterns from a 200 nm thick Ta test chart and a micrometer-sized particle of sulfurized polymer were collected. Subsequently, phase images were reconstructed with resolutions of sub-20 nm and sub-50 nm, respectively. In the near future, tender X-ray PCDI with sub-10 nm resolution is anticipated to potentially revolutionize the visualization of nanoscale structures and chemical states in various functional materials composed of light elements.
PERANCANGAN WORM GEAR MAINFERMENTOR POMPA SENTRIFUGAL DI PT. CHAIL JEDANG INDONESIA
Authors: Arif Rif’an 
Published: May 2024

Roda gigi adalah salah satu bentuk sistem transmisi yang mempunyai fungsi mentransmisikan gaya, membalikkan putaran, mereduksi atau menaikkan putaran/kecepatan. Roda gigi cacing ialah suatu elemen transmisi yang dapat meneruskan daya dan putaran pada poros yang bersilang dan mempunyai gigi yang dipotong menyudut seperti pada roda gigi helik dan dipasangkan dengan ulir yang dinamakan ulir cacing. Dalam perancangan dimensi rodagigi cacing dengan tipe E dengan jarak antar poros a = 200 mm, rasio reduksi i = 10 dengan mengambil nilai z1 = 3 dan zm2 = 25 didapatkan Modul (m): 12 mm, Diameter Minor: 55 mm, Diameter Rata: 85 mm, Diameter Kepala: 104 mm, Diameter Luar: 116 mm, Diameter Pitch: 82 mm, Lebar Gigi: 152 mm, Faktor Gigi: 6,25 mm, Pitch H: 104,5 mm, Sudut kisar rata–rata: 22 mm, Sudut kisar: 22 mm, Modul normal: 10 mm, Sudut heliks wheel: 68 mm. Untuk material yang dipilih adalah paduan Aluminium dengan ketahanan temperatur pada worm (𝑁𝑇) sebesar 3,75 dan ketahanan permukaan pada worm (𝑁1𝐹) sebesar 25 HP, dan ketahanan permukaan pada wheel (𝑁2𝐹) sebesar 18,27 HP, Efisiensi roda gigi berdasarkan kekuatan permukaan sebesar 75 % dan efisiensi roda gigi berdasarkan ketahanan temperatur sebesar 65 % dengan Nilai keamanan terhadap patah gigi sebesar 𝑆𝐵 = 2,96 ≥ 1.
PENYULUHAN DAGUSIBU OBAT PADA SISWA SMAN 1 PARONGPONG
Authors: Sopian Ahmad , Oktaviani Dea Tri , Agustina Selima Aileen , Riadi Resti Fauzi , Sofiatus S Deviana Imanda , Jaojah Siti , Aditya Rindang , Nuri Maharani Anggi 
Published: May 2024

In today's society, substance abuse is rampant, both prescription drugs and drugs purchased on their own initiative. One of the problems associated with drug use that is often encountered in the community is errors in self-medication or self-medication practices caused by a lack of knowledge about drugs and their use, so that in some cases poisoning and drug overdose can cause death. This counseling aims to increase the knowledge and understanding of the community regarding how to obtain, use, store and dispose of drugs properly. The method used in this research is by counseling to students of SMAN 1 Parongpong. DAGUSIBU counseling was held on Friday, November 24, 2023 at SMAN 1 Parongpong with 31 participants. The first counseling began with an explanation of the general definition of drugs and drug classification, the second explanation of how to get drugs properly and correctly, the third explanation of how to use drugs properly and correctly, the last explanation is about how to dispose of drugs properly and correctly. This activity increased the participants' knowledge of how to manage drugs properly and can increase public knowledge

Keywords: Medicine, Use, Management
The Fictitious Constitution of People's Republic of Albania (1946)
Authors: Tokrri Renata 
Published: May 2024

In 1946, in Albania, the Constitution of the People's Republic came into force (reworked in 1950). This Constitution recognized a series of rights and freedoms, such as that of expression, of the press, of assembly, of association, the right to publicly express one's thoughts, freedom of religion, of conscience, etc. Despite the explicit provisions on religion and conscience, the communist regime began a real ideological battle to repress religious sentiment. The State adopted persecutory policies against religions. Persecutions which they pursued in the light of the Fundamental Charter with the aim of eradicating religions from the souls of Albanians. Without taking into consideration that beyond the feelings rooted in each person, beliefs were part of customs and customs. For this reason, anti-religious policy begins with the weakening of the clergy and then proceeds with state atheism. This was only the beginning of the Albanian nightmare, against religion and consequently against all religious communities located in the country.

Received: 10 March 2024 / Accepted: xx April 2024 / Published: 5 May 2024
The Stalemate Over the Negotiation Process of the Grand Ethiopian Renaissance Dam (GERD)
Authors: Wedajo Yohannes Girma 
Published: May 2024

The paper examines the challenges that encounter the negotiation process over the Grand Ethiopian Renaissance Dam (GERD). The project would make a huge contribution to Ethiopia’s economic development and energy demand, though Egypt and Sudan are concerned and seek a binding agreement on the dam’s filling and operation. The dam has become a source of contention between Ethiopia, Egypt and Sudan. To de-escalate the intensity and reach a final and comprehensive deal, the three countries have negotiated multiple times, though it usually ends in disagreement. Position, colonial era agreements, securitizing, and internationalizing the GERD have been obstructing the negotiation. The article suggests that since distributive negotiation neither benefits Ethiopia nor Egypt and Sudan, the talks should be concluded with integrative ends. The parties should also have political will and commitment to make the GERD a plat form for cooperation rather than conflict.

Received: 13 March 2024 / Accepted: 30 April 2024 / Published: 5 May 2024
Evaluation of Herbal Composite for Activity Against Anti-cancer Drug-induced Nephrotoxicity in Wistar Rats
Authors: Narawade Akshay , Janjire Omkar , Undale Vaishali , V Addepalli , Ingale Suvarna , Karwa Pawan N , Neve Vrushali 
Published: May 2024

Background: Nephrotoxicity is among the significant side effects and a dose-restriction mechanism for cisplatin treatment. According to recent studies, Oxidative stress and inflammation are the causative agents for acute renal failure on Cisplatin. Curcumin and resveratrol are considered to have potent anti-inflammatory and antioxidant effects. The current investigation aimed to determine whether a composite of curcumin and resveratrol attenuates nephrotoxicity caused by Cisplatin in Wistar rats.
Method: The herbal compound was administered together with 5 mg/kg of cisplatin daily for 10 days with doses of 100 and 400 mg/kg. To evaluate renal damage, serum creatinine, nitrogen in blood urea, Creatinine, and levels of nitrite were measured. The levels of renal malondialdehyde, decreased glutathione levels, catalase, and also the superoxide dismutase enzyme were measured to quantify renal oxidative stress.
Result: Significant oxidative stress, severe inflammation, and nephrotoxicity were all seen after a single dosage of cisplatin. In Wistar rats, a herbal composite treatment significantly reduced inflammation, improved renal function in a dose-dependent manner, reduced oxidative stress, and provided protection from the nephrotoxicity caused by Cisplatin.
Conclusion: The herbal composite of curcumin - and - resveratrol was found to act as a therapeutic adjutant against Cisplatin nephrotoxicity.
Development of Hospital Formulary for an Emergency Drug List in Tertiary Care Teaching Hospital
Authors: Hiremath Jayasheela S , Viswanatha Swamy Agadi Hiremath , Nyamagoud Sanatkumar Bharamu , Reddy P Pavan Kumar , Bullapur Prasannakumar C , K Vinod , Patil Shanmukh V 
Published: May 2024

Background: In an emergency situation, timing is an important factor that requires sound knowledge about the patient's health condition, diagnosis, and treatment, as well as the administration of an appropriate drug at the appropriate time, which helps save a patient’s life. To ease the workflow, we decided to develop an emergency drug formulary. The study aims to develop a hospital formulary for an emergency drug list and compare it with the WHO standard formulary and the National Formulary of India.
Methods: A prospective observational study was carried out at Vivekananda General Hospital, Hubballi, for a period of six months. A monograph of emergency drugs was drafted based on the needs of the hospital and prepared by referring to various different sources of data. The developed hospital formulary was made into electronic copies and handbooks, and a review was obtained from healthcare professionals.
Results: The results showed that there were 130 drugs in the emergency drug formulary. It includes various categories of medication given in the case of different emergency conditions (cardiac emergencies, neurological emergencies, endocrine emergencies, poisoning emergencies, respiratory emergencies, fluid and electrolyte emergencies, and miscellaneous emergency drugs). While comparing the contents of the monograph with other formulary monographs, we found that the formulary that we had developed was more effective. There were five fixed-dose combinations (FDCs), 27 drugs that are safe to use during pregnancy, 84 drugs that should be used with caution while pregnant, and 1 drug that was contraindicated. We also found that there were 59 drugs that were compatible with breastfeeding; 63 needed to be used with caution; and 12 should be avoided during breastfeeding.
Conclusion: The developed emergency formulary acts as a quick reference, helps in selecting the appropriate drug at the appropriate dose and time, and also helps in selecting low-cost alternative brands.
Żyto jako bogate źródło związków prozdrowotnych
Authors: Gzowska Marlena , Fraś Anna , Wiśniewska Magdalena 
Published: May 2024

Żyto jest gatunkiem o największej zawartości związków bioaktywnych wśród zbóż, co sprawia, że jest bardzo wartościowym składnikiem naszego pożywienia. Pomimo, iż stanowi drugie pod względem ważności zboże chlebowe, nadal pozostaje niedocenione. Spośród powszechnie spożywanych zbóż, gatunek ten zawiera najwięcej błonnika pokarmowego, a także kwasów fenolowych i alkilorezorcynoli. W pożywieniu obecność błonnika pokarmowego i substancji bioaktywnych współtowarzyszących przynosi wiele korzyści związanych z profilaktyką zdrowotną. Substancje te wpływają korzystnie na gospodarkę lipidową organizmu, metabolizm węglowodanów oraz prawidłowe funkcjonowanie przewodu pokarmowego, co w efekcie zmniejsza ryzyko wystąpienia wielu chorób, takich jak miażdżyca, otyłość i cukrzyca.
Celem podjętych badań było określenie składu chemicznego ziarna sześciu odmian żyta ozimego. W badaniach skupiono się na określeniu zawartości składników odżywczych: białka, skrobi, lipidów i związków mineralnych oraz błonnika pokarmowego, alkilorezorcynoli i kwasów fenolowych, stanowiących składniki bioaktywne o działaniu prozdrowotnym. Odmiany wyróżniające się pod względem zawartości składników odżywczych to Dańkowskie Skand i Reflektor, natomiast największą ilość związków bioaktywnych stwierdzono w odmianach KWS Dolaro i Poznańskie. Profil chemiczny ziarna tych odmian cechował się największą zawartością błonnika pokarmowego w tym arabinoksylanów ekstrahowalnych w wodzie i związków fenolowych. Otrzymane wyniki pozwolą wskazać odmiany o najlepszej wartości użytkowej.
Estrés Laboral y Funciones Ejecutivas en docentes de Centro de Atención Integral a la Primera Infancia (CAIPI) del distrito de Panamá
Authors: Noriega-Martínez Gabriela , Gálvez Amores Ida Graciela , Martínez Mantovani Marianne , Montenegro Markelda 
Published: May 2024

El estrés es una forma de respuesta fisiológica ante cambios que causen tensión física, emocional y psicológica cuando nos enfrentamos a distintos retos de la vida, entre ellos el trabajo. El estrés crónico puede traer consecuencias nocivas para la salud integral física y emocional, sobre todo en condiciones adversas como la pandemia por COVID-19. Además, puede causar deterioro en las funciones ejecutivas, decisivas para responder de forma adaptativa a las demandas de nuestro ambiente. Luego del inicio de la pandemia, los docentes de centros de la primera infancia se volvieron parte de la fuerza trabajadora esencial, y se reportaron desmejoras en varios aspectos de su vida. Garantizar condiciones laborales adecuadas, podría no solo mejorar el trabajo y calidad de vida de los docentes, sino también la de sus alumnos. Objetivo: Analizar la relación entre el nivel de estrés docente y las funciones ejecutivas en docentes de Centros de Atención Integral a la Primera Infancia (CAIPI) del distrito de Panamá. Metodología y Muestra: Este fue un estudio cuantitativo, no experimental, con alcance correlacional y diseño de investigación transversal de una sola aplicación. Se incluyeron 67 docentes CAIPI que laborarán en el distrito de Panamá. A estos se les evaluó con una entrevista clínica junto a la Evaluación Conductual de la Función Ejecutiva (BRIEF®-A) y la Escala de Estrés Docente ED-6. Resultados y Conclusiones: Se observaron relaciones moderadas (r= 0.40-0.69) entre algunos factores del estrés establecidos por el ED-6 (ansiedad, depresión, creencias desadaptativas, presiones y desmotivación) y algunas funciones ejecutivas de acuerdo con el BRIEF®-A (iniciación, memoria de trabajo, control emocional y cambio). Estos hallazgos dan información acerca de la complejidad del trabajo docente y podrían brindar herramientas para crear una ruta hacia la formación en funciones ejecutivas y habilidades de control emocional como factor protector para el estrés docente. Esto a su vez podría tener un impacto significativo en el desarrollo de los niños y mejorar la dinámica de enseñanza-aprendizaje.
Strategi Komunikasi Efektif Motor Honda dalam Branding, Marketing dan Selling Produk
Authors: Sukma Agus Hitopa , Misnan Misnan , Hiswanti Hiswanti , Pranawukir Iswahyu , Barizki Rezzi Nanda 
Published: May 2024

Industri otomotif di Indonesia saat ini sangatlah kompetitif, dengan berbagai merek yang bersaing untuk mendapatkan perhatian konsumen. Dalam situasi ini, strategi komunikasi yang efektif menjadi kunci untuk membangun brand, memasarkan produk, dan meningkatkan penjualan. Penelitian ini bertujuan untuk menganalisis strategi komunikasi yang digunakan PT. Astra Honda Motor (AHM) dalam branding, marketing, dan selling produk motor Honda di Indonesia. Penelitian ini menggunakan metode kualitatif dengan studi kasus pada AHM. Data dikumpulkan melalui wawancara dengan informan kunci di AHM, observasi dan analisis dokumen. Hasil penelitian menunjukkan bahwa AHM menggunakan berbagai strategi komunikasi yang efektif dalam branding, marketing, dan selling produk motor Honda. Strategi branding meliputi pembentukan brand image yang kuat. AHM telah membangun brand image Honda sebagai motor yang berkualitas, irit bahan bakar dan sporty. Hal ini dilakukan melalui berbagai kegiatan komunikasi, seperti iklan, sponsorship dan event. Strategi marketing dilakukan melalui pemasaran yang terintegrasi. Strategi tersebut meliputi: 1) Above-the-line communication: AHM menggunakan berbagai media iklan seperti TV, radio, media cetak, dan media online untuk membangun brand awareness dan meningkatkan minat konsumen terhadap produk motor Honda. 2) Below-the-line communication: AHM menggunakan berbagai kegiatan promosi seperti pameran, event dan sponsorship untuk meningkatkan penjualan produk motor Honda. 3) Digital marketing: AHM memanfaatkan media digital seperti website, media sosial, dan aplikasi mobile untuk menjangkau konsumen yang lebih luas dan menjalin interaksi yang lebih personal dengan konsumen. Adapun strategi selling diutamakan penjualan secara personal (personal selling). AHM memiliki jaringan dealer yang luas di seluruh Indonesia. Dealer-dealer ini menyediakan layanan penjualan dan purna jual yang berkualitas kepada konsumen.