bluuebunny/crossref_metadata_2025_split huggingface dataset:
MODEL-MODEL PEMBELAJARAN MENULIS ANAK USIA DINI DI RAUDHATUL ATHFAL SE-KOTA MEDAN T.A 2021/2022
Authors: P Harahap Muhammad Nazri , Sapri Sapri , Sitorus Ahmad Syukri 
Published: June 2024

Tujuan dari penelitian ini yaitu 1) untuk mengetahui model-model menulis anak usia dini di RA Se-kota Medan, 2) untuk mengetahui faktor-faktor pendukung dan penghambat kemampuan menulis di RA Se-kota Medan. Jenis penelitian yang digunakan yaitu penelitian kualitatif dengan deskriptif analisis. Metode dalam mengumpulkan data dengan teknik wawancara, obsevasi dan dokumentasi. Data yang digunakan pada penelitian ini dengan subjek penelitian yaitu dengan guru-guru RA Se-kota Medan. Adapun hasil penelitian ini menunjukkan bahwa model-model pembelajaran menulis anak usia dini di Raudhatul Athfal Se-kota Medan T.A 2021/2022 yaitu 1) Model-model menulis anak usia dini di RA Se-kota Medan yaitu dilakukan di RA Ibunda, RA Bunayya IV, dan RA Ar-Rayhan dengan menggunakan model pembelajaran pramenulis dan model draf. Dengan model ini, anak terlebih dahulu di latih tangannya dengan bermain plastisin, meremas kertas, bermain pasir, melempar, dan menangkap bola. Adapun bentuk reward diberikan kepada anak agar anak semakin senang dalam pembelajaran menulis. 2) Faktor pendukung pembelajaran yaitu dukungan dari guru, orang tua dan lingkungan keluarga. Ini semua sangat berpengaruh, karena itu guru harus memberikan media yang baik untuk meningkatkan kemampuan menulis anak seperti media puzzle huruf, memberikan media kertas untuk anak mencoret-coret dibuku kasar, dan memberikan reward juga pujian agar menumbuhkan semangat anak. Dan faktor penghambatnya adalah kurangnya kompetensi guru dalam memberikan pembelajaran untuk menstimulus anak dalam memahami huruf dan angka, anak mudah bosan dalam menullis, anak kurang konsentrasi. Diharapkan dengan adanya penelitian ini dapat membantu perkembangan anak dalam meningkatkan kemampuan menulis anak di RA Se-kota Medan dengan menggunakan model-model pembelajaran yang menarik
PENERAPAN METODE OUTBOUND TERHADAP KEMAMPUAN BERSOSIALISASI ANAK PADA PAUD INSAN RABBANI
Authors: Sarifah Sarifah , Agusriani Ade , Tahir M. Yusuf 
Published: June 2024

Penelitian ini bertujuan untuk mengetahui peningkatan kemampuan bersosialisasi anak setelah penerapan metode outbound. Adapun rumusan masalah pada penelitian ini yaitu bagaimana metode outbound diterapkan dalam meningkatkan kemampuan bersosialisasi anak?. Jenis penelitian ini adalah penelitian tindakan kelas (classroom action research). Subjek yang digunakan dalam penelitian inia adalah 12 orang anak. Data penelitian tindakan kelas ini dikumpulkan melalui metode observasi menggunakan lembar observasi. Analisis data menggunakan analisis statistik deskriptif. Penelitian ini dilaksanakan dengan dua Siklus yaitu Siklus I dan Siklus II yang terdiri dari tahap perencanaan, pelaksanaan, Pengamatan/observasi, dan refleksi. Hasil penelitian yang diperoleh pada pra tindakan menunjukan bahwa rata-rata presentase kemampuan bersosialiasi anak berada pada kriteria kurang yaitu 75%, setelah itu dilakukan tindakan pada Siklus I sehingga terjadilah peningkatan kemampuan bersosialissasi dari rata-rata presentase 0% anak yang memiliki kemampuan bersosialisasi dengan kriteria baik meningkat menjadi rata-rata presentase 16,67% anak, namun pada Siklus I belum sampai hasil pada kriteria keberhasilan sehingga dilakukan tindakan pada Siklus II Dan mengalami peningkatan rata-rata presentase menjadi 83,33%. Hal ini menunjukan bahwa ada peningkatan kemampuan bersosialisasi anak dari Siklus I hingga Siklus II. Jadi penerapan metode outbound dapat meningkatkan kemampuan bersosialisasi anak pada PAUD Insan Rabbani Desa Soki Kecamatan Belo Kabupaten Bima.
menumbuhkan jiwa nasionalisme anak usia dini melalui kegiatan bermain dengan media lapbook
Authors: Handayani Dewie Hartati , Suryani Lilis 
Published: June 2024

Penelitian ini bertujuan untuk mencermati upaya menumbuhkan jiwa nasionalisme anak usia dini melalui kegiatan bermain dengan menggunakan media lapbook. Penelitian dilakukan di Homeschooling Adiba Palembang, selama kurun waktu 3 bulan, yakni bulan September- Desember 2023. Berdasarkan jenisnya, penelitian ini merupakan penelitian kualitatif deskritif. Metode pengumpulan data pada penelitian ini melalui tiga model, yakni wawancara, observasi dan dokumentasi. Data yang diperoleh memiliki keabsahan dan memiliki derajad kepercayaan, maka peneliti menggunakan dua teknik, yakni ketekunan pengamatan dan trianggulasi data. Teknik analisis data yang digunakan adalah metode Miles and Huberman yang terdiri dari tahap pengumpulan data, reduksi data, penyajian data dan penarikan kesimpulan. Hasil penelitian ini menunjukkan bahwa kegiatan bermain menggunakan media lapbook dapat dijadikan sebagai sarana untuk menumbuhkan jiwa nasionalisme anak usia dini. Hal tersebut diantaranya tercermin dari sikap kemandirian, toleransi, saling menghargai, budaya antri, serta perasaan menjadi bagian dari sejarah keindonesiaan.
Evolution of long-term ecological security pattern of island city and its influencing factors—a case study in Pingtan Island
Authors: Wu Binsheng , Dai Kunli , Liu Chunqing , Chen Jingru 
Published: June 2024

Introduction: As the global urbanization process accelerates, the contradiction between economic development demands and ecological protection becomes increasingly prominent.Methods: In this study, we simulated the evolution of the ecological security pattern (ESP) of Pingtan Island from 2000 to 2020 by extracting the ecological sources using Remote Sensing Ecological Index (RSEI), and identifying the ecological corridors and key nodes by combining with Linkage Mapping (LM) and Circuit Theory. In addition, Geodetector was utilized to identify these major determinants affecting RSEI.Results: The results showed 1) From 2000 to 2020, the ecological environmental quality (EEQ) of Pingtan Island continued to improve, and the mean value of RSEI gradually increased from 0.47 to 0.51. 2) Univariate analysis showed that elevation and slope were the most significant factors affecting the spatial variability of the RSEI, with the interaction between slope and proportion of built-up area having a significant effect on EEQ. 3) The number and extent of ecological sources were expanded year by year with significant spatial variability. At the same time, the number and range of ecological corridors also underwent phase adjustment. 4) Further exploration of ESP of Pingtan Island in 2020 identified 32 ecological pinch points (EPPs) and 52 ecological barrier points (EBPs), which were mainly located within or near the ecological corridors, indicating key areas for future ecological restoration efforts.Discussion: These insights help to enhance urban spatial planning and ecosystem restoration on Pingtan Island and provide a blueprint for ESP development in comparable island urban environments.
The function of the complement system remains fully intact throughout the course of allogeneic stem cell transplantation
Authors: Fageräng Beatrice , Cyranka Leon , Schjalm Camilla , McAdam Karin Ekholt , Larsen Carina Sandem , Heinzelbecker Julia , Gedde-Dahl Tobias , Würzner Reinhard , Espevik Terje , Tjønnfjord Geir Erland , Garred Peter , Barratt-Due Andreas , Tvedt Tor Henrik Anderson , Mollnes Tom Eirik 
Published: June 2024

IntroductionHematopoietic stem cell transplantation (HSCT) is associated with immune complications and endothelial dysfunction due to intricate donor-recipient interactions, conditioning regimens, and inflammatory responses.MethodsThis study investigated the role of the complement system during HSCT and its interaction with the cytokine network. Seventeen acute myeloid leukemia patients undergoing HSCT were monitored, including blood sampling from the start of the conditioning regimen until four weeks post-transplant. Clinical follow-up was 200 days.ResultsTotal complement functional activity was measured by WIELISA and the degree of complement activation by ELISA measurement of sC5b-9. Cytokine release was measured using a 27-multiplex immuno-assay. At all time-points during HSCT complement functional activity remained comparable to healthy controls. Complement activation was continuously stable except for two patients demonstrating increased activation, consistent with severe endotheliopathy and infections. In vitro experiments with post-HSCT whole blood challenged with Escherichia coli, revealed a hyperinflammatory cytokine response with increased TNF, IL-1β, IL-6 and IL-8 formation. Complement C3 inhibition markedly reduced the cytokine response induced by Staphylococcus aureus, Aspergillus fumigatus, and cholesterol crystals.DiscussionIn conclusion, HSCT patients generally retained a fully functional complement system, whereas activation occurred in patients with severe complications. The complement-cytokine interaction indicates the potential for new complement-targeting therapeutic strategies in HSCT.
Development and validation of UPLC-MS/MS method for icariin and its metabolites in mouse urine
Authors: Li Na , Yuan Mei , Che Jinjing 
Published: June 2024

An ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method was utilized to develop a technique for the simultaneous quantification of icariin and its primary metabolites in mouse urine. The levels of icariin, icariside Ⅰ, icariside Ⅱ, baohuoside Ⅱ, wushanicaritin, icaritin, and desmethylicaritin in mouse urine were analyzed subsequent to the oral administration of an icariin suspension. This study aimed to preliminarily investigate the excretion profile of icariin in mice. Using an aqueous solution containing 0.1% formic acid (A) and an acetonitrile solution containing 0.1% formic acid (B) as the mobile phases, icariin and its major metabolites demonstrated satisfactory linearity over the concentration range of 0.25–800 ng·mL−1. The precision and accuracy of intra-day and inter-day measurements were all found to be within 15%. Seventy-two hours after the intragastric administration of icariin suspension to a mouse, the cumulative urinary excretion of icariin, icariside Ⅰ, icariside Ⅱ, baohuoside Ⅱ, wushanicaritin, icaritin, and desmethylicaritin was quantified as 13.48, 18.70, 2,627.51, 2.04, 10.04, 3,420.44, and 735.13 ng, respectively. The UPLC-MS/MS method developed in this research is characterized by its simplicity, sensitivity, and speed, making it well-suited for the concurrent quantification of icariin and its associated metabolites in urine. Additionally, it is appropriate for analyzing urine samples that may contain multiple drugs in future investigations.
Caffeine regulates both osteoclast and osteoblast differentiation via the AKT, NF-κB, and MAPK pathways
Authors: Miao Yue , Zhao Lei , Lei Shuwen , Zhao Chunyan , Wang Qiuping , Tan Chao , Peng Chunxiu , Gong Jiashun 
Published: June 2024

Background: Although caffeine generally offers benefits to human health, its impact on bone metabolism remains unclear.Aim and Methods: This study aimed to systematically evaluate the long-term effects of caffeine administration on osteoclasts, osteoblasts, and ovariectomy-induced postmenopausal osteoporosis (OP).Results: Our in vitro findings revealed that 3.125 and 12.5 μg/mL caffeine inhibited RANKL-mediated osteoclastogenesis in RAW 264.7 cells through the MAPK and NF-κB pathways, accompanied by the inactivation of nuclear translocation of nuclear factor NFATc1. Similarly, 3.125 and 12.5 μg/mL of caffeine modulated MC3T3-E1 osteogenesis via the AKT, MAPK, and NF-κB pathways. However, 50 μg/mL of caffeine promoted the phosphorylation of IκBα, P65, JNK, P38, and AKT, followed by the activation of NFATc1 and the inactivation of Runx2 and Osterix, ultimately disrupting the balance between osteoblastogenesis and osteoclastogenesis. In vivo studies showed that gavage with 55.44 mg/kg caffeine inhibited osteoclastogenesis, promoted osteogenesis, and ameliorated bone loss in ovariectomized mice.Conclusion: Conversely, long-term intake of high-dose caffeine (110.88 mg/kg) disrupted osteogenesis activity and promoted osteoclastogenesis, thereby disturbing bone homeostasis. Collectively, these findings suggest that a moderate caffeine intake (approximately 400 mg in humans) can regulate bone homeostasis by influencing both osteoclasts and osteoblasts. However, long-term high-dose caffeine consumption (approximately 800 mg in humans) could have detrimental effects on the skeletal system.
Genome-wide analysis of the MADS-box gene family of sea buckthorn (Hippophae rhamnoides ssp. sinensis) and their potential role in floral organ development
Authors: Zhao Jing , Xu Yazhuo , Zhang Zhihua , Zhao Meng , Li Kai , Wang Fanhong , Sun Kun 
Published: June 2024

Sea buckthorn (Hippophae rhamnoides ssp. sinensis) is a deciduous shrub or small tree in the Elaeagnaceae family. It is dioecious, featuring distinct structures in female and male flowers. The MADS-box gene family plays a crucial role in flower development and differentiation of floral organs in plants. However, systematic information on the MADS-box family in sea buckthorn is currently lacking. This study presents a genome-wide survey and expression profile of the MADS-box family of sea buckthorn. We identified 92 MADS-box genes in the H. rhamnoides ssp. Sinensis genome. These genes are distributed across 12 chromosomes and classified into Type I (42 genes) and Type II (50 genes). Based on the FPKM values in the transcriptome data, the expression profiles of HrMADS genes in male and female flowers of sea buckthorn showed that most Type II genes had higher expression levels than Type I genes. This suggesting that Type II HrMADS may play a more significant role in sea buckthorn flower development. Using the phylogenetic relationship between sea buckthorn and Arabidopsis thaliana, the ABCDE model genes of sea buckthorn were identified and some ABCDE model-related genes were selected for qRT-PCR analysis in sea buckthorn flowers and floral organs. Four B-type genes may be involved in the identity determination of floral organs in male flowers, and D-type genes may be involved in pistil development. It is hypothesized that ABCDE model genes may play an important role in the identity of sea buckthorn floral organs. This study analyzed the role of MADS-box gene family in the development of flower organs in sea buckthorn, which provides an important theoretical basis for understanding the regulatory mechanism of sex differentiation in sea buckthorn.
Multifaceted insights into the environmental adaptability of Arnebia guttata under drought stress
Authors: Liu Qian , Liu Haolin , Zhang Min , Lv Guoshuai , Zhao Zeyuan , Chen Xingyu , Wei Xinxin , Zhang Chunhong , Li Minhui 
Published: June 2024

IntroductionGlobal warming has led to increased environmental stresses on plants, notably drought. This affects plant distribution and species adaptability, with some medicinal plants showing enhanced drought tolerance and increased medicinal components. In this pioneering study, we delve into the intricate tapestry of Arnebia guttata, a medicinal plant renowned for its resilience in arid environments. By fusing a rich historical narrative with cutting-edge analytical methodologies, this research endeavors to demystify the plant’s intricate response to drought stress, illuminating its profound implications for medicinal valorization.MethodsThe methodology includes a comprehensive textual research and resource investigation of A. guttata, regionalization studies, field sample distribution analysis, transcriptome and metabolome profiling, rhizosphere soil microbiome analysis, and drought stress experiments. Advanced computational tools like ArcGIS, MaxEnt, and various bioinformatics software were utilized for data analysis and modeling.ResultsThe study identified significant genetic variations among A. guttata samples from different regions, correlating with environmental factors, particularly precipitation during the warmest quarter (BIO18). Metabolomic analysis revealed marked differences in metabolite profiles, including shikonin content, which is crucial for the plant’s medicinal properties. Soil microbial community analysis showed variations that could impact plant metabolism and stress response. Drought stress experiments demonstrated A. guttata’s resilience and its ability to modulate metabolic pathways to enhance drought tolerance.DiscussionThe findings underscore the complex interplay between genetic makeup, environmental factors, and microbial communities in shaping A. guttata’s adaptability and medicinal value. The study provides insights into how drought stress influences the synthesis of active compounds and suggests that moderate stress could enhance the plant’s medicinal properties. Predictive modeling indicates future suitable growth areas for A. guttata, aiding in resource management and conservation efforts. The research contributes to the sustainable development of medicinal resources and offers strategies for improving the cultivation of A. guttata.
Navigating foster care: how parental drug use and caregiver attitudes shape children’s mentalization processes—an exploratory longitudinal follow-up study: study protocol
Authors: Springer Nadja , Lueger-Schuster Brigitte 
Published: June 2024

BackgroundThe current research concept of mentalization is used in the study to clearly identify affective and cognitive abilities of the caregiver-child dyad with the aim of compensating deficits on both sides with psychological-psychotherapeutic strategies.MethodsThe objective of this explorative, longitudinal intervention study is to provide an in-depth understanding of the psycho-social background of 30 children aged 6–12 years living in institutional or family-centered foster care. Data will be collected at three time points: before, after and 12 months after participating in the newly developed group intervention, which intends to address the particular needs of children of drug abusing parents living in foster care in the latency period. The study is conducted at the Faculty of Psychology of the University of Vienna in collaboration with the Association “Dialogue” (Verein Dialog). The treatment duration spans 5 months, during which two specifically trained psychotherapists conduct 10 group sessions for children and three group sessions for foster caregivers. All statistical analyses will consider the type of data available. Therefore, the primary outcome of the study will be assessed via the Friedman test due to the ordinal dependent variable as it is the non-parametric alternative to the one-way ANOVA for repeated measures. In addition, the Mann–Whitney U test is used to compare differences between two independent groups (children living in institutional foster care vs. family foster care). To assess potential correlations regarding the child and caregivers’ capacity to mentalize, Spearman correlations (ρ) are conducted. To examine the secondary outcome, apart from the methods previously outlined, we will also utilize qualitative thematic analysis.DiscussionThe present study uses the current research concept of mentalization to identify affective and cognitive abilities of the caregiver-child dyad with the aim of compensating deficits on both sides with psychological-psychotherapeutic strategies. There are some limitations of the study to mention: the small sample size does not allow to generalize the results. Due to the lack of a comparison group, a randomized control study (RCT) was not conducted. The authors are aware of these limitations. However, the studies’ findings, will help to deduce research questions for further studies.
Neural activation during natural speech and rests in patients with schizophrenia and schizophrenia spectrum disorders—an fMRI pilot trial
Authors: Hahn Wiebke , Tsalouchidou Panagiota-Eleni , Nagels Arne , Straube Benjamin 
Published: June 2024

BackgroundIn schizophrenia patients, spontaneous speech production has been hypothesized as correlating with right hemispheric activation, including the inferior frontal and superior temporal gyri as speech-relevant areas. However, robust evidence for this association is still missing. The aim of the present fMRI study is to examine BOLD signal changes during natural, fluent speech production in patients with schizophrenia in the chronic phase of their disease.MethodsUsing a case–control design, the study included 15 right-handed patients with schizophrenia spectrum disorders as well as 15 healthy controls. The participants described eight pictures from the Thematic Apperception Test for 1 min each, while BOLD signal changes were measured with 3T fMRI. The occurrence of positive and negative formal thought disorders was determined using standardized psychopathological assessments.ResultsWe found significant BOLD signal changes during spontaneous speech production in schizophrenia patients compared to healthy controls, particularly in the right hemispheric network. A post-hoc analysis showed that this right-hemispheric lateralization was mainly driven by activation during experimental rests. Furthermore, the TLI sum value in patients correlated negatively with BOLD signal changes in the right Rolandic operculum.ConclusionsPossible underlying factors for this inverse right-hemispheric lateralization of speech-associated areas are structural changes and transmitter system alterations, as well as a lack of neural downregulation in schizophrenia patients during rest periods due to dysfunctional executive functions. When examining spontaneous speech as the most natural form of language, other influencing factors, such as social cognition or emotional processing, should be considered. Our results indicate that future studies should consider group differences during rest, which might provide additional information typically covered in differential contrasts.