bluuebunny/crossref_metadata_2025_split huggingface dataset:
The salivary peroxidase system: thermodynamic, kinetic and antibacterial properties
Authors: Pruitt K. M. 
Published: August 2010

Pruitt KM. The salivary peroxidase system: thermodynamic, kinetic and antibacterial properties. J Oral Pathol 1987: 16: 417–420.Enzymes are specific catalysts which optimize the rates of reactions vital to living organisms. If the oxidation of thiocyanate (which occurs, in vivo, in human saliva and salivary glands) is a vital reaction for the maintenance of oral health, then the enzyme (salivary peroxidase), which catalyzes this reaction, should function optimally under in vivo conditions. Studies have shown that salivary peroxidase maintains the SCN‐ oxidation reaction in an apparent state of dynamic equilibrium, that the kinetic properties of the enzyme provide optimum rates of reaction under in vivo conditions, and that the antibacterial properties of the products of the reaction are optimum under those conditions where unlimited bacterial metabolism provides the greatest threat to host tissues. The evidence indicates that the salivary peroxidase enzyme has evolved in such a way as to maximize its protective value in the oral cavity.
Prevalence of Candida species in AIDS patients and HIV‐free subjects in Thailand
Authors: Teanpaisan R. , Nittayananta W. 
Published: August 2010

Teanpaisan R, Nittayananta W: Prevalence of Candida species in AIDS patients and HIV‐free subjects in Thailand. J Oral Pathol Med 1998; 27: 4–7. © Munksgaard, 1998.The purpose of this study was to examine the prevalence of Candida species among groups of HIV‐infected and HIV‐free subjects in Thailand and to ascertain whether particular Candida species were associated with HIV infection. Oral rinse specimens were collected from 45 AIDS patients (CDC stage IV), 74 HIV‐free healthy subjects, and 42 HIV‐free patients who had clinical candidiasis. Yeasts recovered in culture were identified and quantified. The mean ages of the cohorts were 30.75 ± 8.19 years (AIDS group), 28.50 ± 7.98 (HIV‐free healthy group) and 41.83 ± 12.25 years (HIV‐free candidiasis group). Yeasts were isolated from 30/45 (66.66%, range 6.6 ± 102‐5.7 × 106 CFU/ml) of the AIDS group, 8/74 (10.81%, range 8.0 × 101‐3.5 × 104 CFU/ml) of the HIV‐free healthy group, and 24/42 (57.14%, range l.0 × 10 102‐1.1 × 105 CFU/ml) of the HIV‐free candidiasis group. There were statistically significant differences in the Candida colony counts between the AIDS group without oral candidiasis and the healthy group (P=0.0078) and between the AIDS group with candidiasis and the HIV‐free, oral candidiasis group (P= 0.0003). Candida albicans was the most common species recovered from AIDS patients (29 out of 30; 96.66%).
Significant correlation between matrix metalloproteinase activity and tumor necrosis factor‐oc in salivary extravasation mucoceles
Authors: Hoque Mohammad Obaidul , Azuma Masayuki , Sato Mitsunobu 
Published: August 2010

Hoque MO, Azuma M, Sato M: Significant correlation between matrix metalloproteinase activity and tumor necrosis factor‐a in salivary extravasation mucoceles. J Oral Pathol Med 1998; 27: 30–3. © Munksgaard, 1998.We examined the matrix metalloproteinase (MMP) activity and tumor necrosis factor (TNF)‐a in luminal fluid of 18 extravasation mucoceles and in saliva from Wharton's duct of five patients by means of gelatin zymography and enzyme immunoassay, respectively. The luminal fluid showed a high level of MMP activity compared with the saliva. Quantitative determination by enzyme immunoassay revealed that the luminal fluid contained higher levels of TNF‐a than the saliva. In addition, the amount of TNF‐a in luminal fluid exhibited a direct correlation with MMP activity estimated by densitometric analysis of gelatin zymograms. Since TNF‐a stimulates the production of MMPs from cells such as fibroblasts, these results suggest that TNF‐a is one of the causal molecules that enhance the accumulation of proteolytic enzymes in luminal fluid of mucoceles.
Application of the polymerase chain reaction for the diagnosis of malignant lymphoma of the nasal and oral cavities
Authors: Mishima Kenji , Matsuoka Hiroki , Yamada Eiji , Yoshikawa Takafumi , Shiotani Hiroshi , Takayama Kenichi , Kirita Tadaaki , Yamamoto Kazuhiko , Sugimura Masahito , Ichijima Kunio 
Published: August 2010

Mishima K, Matsuoka H, Yamada E, Yoshikawa T, Shiotani H, Takayama K, Kirita T, Yamamoto K, Sugimura M, Ichijima K: Application of the polymerase chain reaction for the diagnosis of malignant lymphoma of the nasal and oral cavities. J Oral Pathol Med 1998; 27: 43–7. © Munksgaard, 1998.Difficulties are often encountered in the diagnosis of malignant lymphoma of the oral and nasal cavities by histology and immunohistochemistry because these malignancies may be complicated by inflammation and necrosis. In the present study, immunoglobulin heavy chain (IgH) and T‐cell receptor (TCR) gene rearrangements were examined by the polymerase chain reaction (PCR) in formalin‐fixed and paraffin‐embedded specimens obtained from 18 patients; the advantages of this method were evaluated. Twelve of these patients were diagnosed as having malignant lymphoma and six had tumors highly suggestive of malignant lymphoma. Fourteen (78%) of the 18 cases were diagnosed as malignant lymphoma histologically and immunohistochemically. Sixteen (89%) of the 18 cases were shown to be monoclonal by PCR. The four cases that could not be histologically or immunohistochemically diagnosed as malignant lymphoma showed monoclonal gene rearrangement by PCR. As a result, six patients suspected of having a malignant lymphoma were diagnosed as having one. The diagnosis of malignant lymphoma by PCR alone still has various problems. However, when the results of PCR are evaluated together with histopathological and immunohisto‐chemical results, PCR makes a useful contribution to the diagnosis of malignant lymphomas of the nasal and oral cavities.
Epstein‐Barr virus in tobacco‐induced oral cancers and oral lesions in patients from India
Authors: D'Costa Jenice , Saranath Dhananjaya , Sanghvi Vikram , Mehta Ashok R. 
Published: August 2010

D'Costa J, Saranath D, Sanghvi V, Mehta AR: Epstein‐Barr virus in tobacco‐induced oral cancers and oral lesions in patients from India. J Oral Pathol Med 1998; 27: 78–82. © Munksgaard, 1998.We examined 103 oral squamous cell carcinomas (OSCC), 100 oral lesions consisting primarily of leukoplakia (82 cases), and 76 clinically normal mucosa specimens from the contralateral site in the oral cavity of individuals with oral lesions, for the presence of Epstein‐Barr virus (EBV). Polymerase chain reaction (PCR) was used to amplify a 239 bp fragment of the BamHIL region of the EBV genome, followed by Southern blot hybridization with EBV oligonucleotide probe to increase further the specificity and sensitivity of the assay system. Since EBV seropositivity is frequent in populations, we also examined the peripheral blood cells (PBC) from 141 patients (50 oral cancer patients, 91 patients with oral lesions) for the presence of EBV We detected EBV in 25 of 103 (25%) OSCC, 13 of 100 (13%) oral lesions, 3 of 76 (4%) clinically normal mucosa samples and 10 of 141 (7%) PBC. Our results indicate that EBV may contribute as one of the multiple factors in oral cancers, in a certain proportion of Indian patients.
Cytomorphometric analysis of squames obtained from normal oral mucosa and lesions of oral leukoplakia and squamous cell carcinoma
Authors: Ramaesh T. , Mendis B. R. R. N. , Ratnatunga N. , Thattil R. O. 
Published: August 2010

Ramaesh T, Mendis BRRN, Ratnatunga N, Thattil RQ: Cytomorphometric analysis of squames obtained from normal oral mucosa and lesions of oral leukoplakia and squamous cell carcinoma. J Oral Pathol Med 1998; 27: 83–6. © Munksgaard, 1998.Cell and nuclear diameters (CD and ND) were measured in squames obtained from normal buccal mucosa and lesions of oral leukoplakia and squamous carcinoma (SCC) also from buccal mucosa. The study groups consisted of Group 1: normal buccal mucosa (n= 40); Group 2: lesions with no epithelial dysplasia (n= 58); Group 3: lesions with epithelial dysplasia (n= 27); and Group 4: SCC lesions (n= 51). The mean CD and ND values were: Group 1: 51.78 (± 0.11) and 8.36 (± 0.49); Group 2: 45.73 (± 0.16) and 8.31(± 0.68); Group 3: 41.32 (± 0.13) and 9.04 (± 0.46); Group 4: 38.58 (± 0.11) and 10.10 (± 0.56) urn, respectively. Correlation between the ND and CD was positive for Group 1 (r= 0.78, P < 0.05) and Group 2 (r= 0.33, P < 0.05). There were no significant correlations in Groups 3 and 4. ANOVA showed significant differences (P < 0.05) for CD between all four groups. Except between Groups 1 and 2, the ND was significantly different (P < 0.05) between all groups. The results indicate that ND and CD could possibly be sensitive parameters in the diagnosis of oral premalignant and malignant lesions.
Oral lesions in children with perinatally acquired human immunodeficiency virus infection
Authors: Nicolatou Ourania , Theodoridou Maria , Mostrou Glyceria , Velegraki Aristea , Legakis Nicholas J. 
Published: August 2010

ABSTRACTFifteen vertically HIV‐infected children aged between 2 and 12 years were followed up for 1 year, weekly to monthly, to study the incidence of oral lesions. At the time of first examination, oral candidiasis (OC) was observed in nine children. Seven children presented with the erythematous type only and two with pseudomembranous oral candidiasis. Four cases of cheilitis were seen in association with the erythematous forms of oral candidiasis. One erythematous candidiasis progressed to pseudomembranous form. A second case of erythematous OC, after multiple recurrences in the form of erythematous OC, recurred as pseudomembranous OC. Another case of erythematous OC and one of pseudomembranous OC presented after multiple recurrences as a persistent, adherent pseudomembranous OC. An orofacial herpes‐zoster infection, a hairy leukoplakia and a necrotic lingual ulcer were observed as second lesions and in association with oral candidiasis in three children. Erythematous oral candidiasis was the most frequent oral HIV‐related lesion, was observed in different stages of HIV‐infection, and in some cases progressed to pseudomembranous candidiasis. A different, selectively resistant, Candida clone was isolated in three cases of recurrent candidiasis.
Deficiency in collagen and fibronectin phagocytosis by human buccal mucosa fibroblasts in vitro as a possible mechanism for oral submucous fibrosis
Authors: Tsai C. C. , Ma R. H. , Shieh T. Y. 
Published: August 2010

ABSTRACTOral submucous fibrosis (OSF), a chronic oral mucosal condition commonly found in south Asians, is a disorder characterized by a quantitative as well as a qualitative alteration of collagen deposition within the subepithelial layer of the oral mucosa. Since degradation of collagen by fibroblast phagocytosis is an important pathway for physiological remodelling of soft connective tissues, we have investigated phagocytosis of collagen‐ and fibronectin‐coated latex beads by fibroblast cultures with an in vitro model system. Coated fluorescent latex beads were incubated with human oral mucosa fibroblasts and the fluorescence associated with internalized beads was measured by flow cytometry. Cells from normal tissues that had been incubated with beads for 16 h contained a mean of 75% collagen phagocytic cells and 70% fibronectin phagocytic cells; however, about 15% and 10% of phagocytic cells individually contained more than twice the mean number of beads per cell. In contrast, cells from OSF tissues exhibited a 40% reduction of the proportions of collagen phagocytic cells (mean=35%) and a 48% decrease of the proportions of fibronectin phagocytic cells (mean=22%), none of the cells having a high number of beads as compared to normal fibroblasts. OSF lesions appear to contain fibroblasts with marked deficiencies in collagen and fibronectin phagocytosis. To investigate if inhibition of phagocytosis could be demonstrated in vitro, normal fibroblast cultures were incubated with areca nut alkaloids (arecoline, arecaidine). The cultures had a dose‐dependent reduction in the proportions of phagocytic cells. On the other hand, corticosteroid used in the treatment of OSF exhibited a dose‐dependent enhancement in the proportion of phagocytic cells. Therefore, our hypothesis for OSF, although over‐simplified, is that betel nut alkaloids (arecoline, arecaidine) inhibit fibroblast phagocytosis and this provides a mechanism for the development of OSF. The benefit of a local intralesional injection of corticosteroid is also possibly, at least in part, through an enhancement of fibroblast collagen phagocytosis.
Expression of E‐cadherin in oral cancer cell lines and its relationship to invasiveness in SCID mice in vivo
Authors: Hoteiya Toshiaki , Hayashi Eiji , Satomura Kazuhito , Kamata Nobouki , Nagayama Masaru 
Published: August 2010

ABSTRACTWe examined the expression of E‐cadherin in nine oral cancer cell lines. HSC‐4, NA, ZA, HOC927 and Ca9–22 cells strongly expressed E‐cadherin [E‐CD(++) cell line] and HSC‐2 and HSC‐3 cells weakly expressed E‐cadherin [E‐CD(+) cell line]. All the cell lines that expressed E‐cadherin were of cuboidal morphology and formed cobblestone colonies. In contrast, TSU and HOC313 cells had spindle shapes, formed dispersed colonies, and were completely negative for E‐cadherin [E‐CD(‐) cell line]. Moreover, all cell lines that expressed E‐cadherin showed tumorgenicity in SCID mice, but E‐CD(‐) cell lines did not show tumorgenicity. The tumors derived from E‐CD(+) cell lines invaded deeper into the connective tissues than those from E‐CD(++) cell lines. In immunohistochemical analysis, the difference was more marked at the edges of the cancer nests. These results suggest that E‐cadherin expression was relevant to the cell forms and the differential grade of cultured cells and that reduced E‐cadherin in oral cancer may be associated with invasiveness in vivo.
Immunohistochemical evaluation of Fhit protein expression in oral squamous cell carcinomas
Authors: van Heerden W. F. P. , Swart T. J. P. , van Heerden M. B. , van Rensburg E. J. , Engelbrecht S. , Dreyer L. , Huebner K. 
Published: August 2010

van Heerden WFP, Swart TJP, van Heerden MB, van Rensburg EJ, Engelbrecht S, Dreyer L, Huebner K: Immunohistochemical evaluation of Fhit protein expression in oral squamous cell carcinomas. J Oral Pathol Med 1999; 28: 433–7. © Munksgaard, 1999.The expression of Fhit (fragile histidine triad) protein in oral squamous cell carcinoma (OSCC) and adjacent oral epithelium was evaluated by immunohisto‐chemistry on formalin‐fixed paraffin‐embedded blocks of 32 cases of OSCC. Rabbit polyclonal anti‐GST‐Fhit antiserum at 1:600 was used, after antigen enhancement in a microwave pressure cooker, in a saturated lead thiocyanate solution. This antiserum has been shown specifically to detect human Fhit by immunohistochemistry at dilutions up to 1:10,000. The Fhit protein expression was evaluated using both the intensity and extent of staining. Normal stratified squamous epithelium showed strong positivity, especially in the stratum spinosum and areas of keratinisation. Basal and parabasal cells were negative or expressed low levels of Fhit relative to the squamous epithelium. Mild and moderate epithelial dysplasia showed Fhit expression in the superficial layers, while Fhit expression was absent from severely dysplastic lesions. A reduction or loss of Fhit expression was found in 21 (66%) of the OSCC. The alterations in Fhit protein expression in OSCC, and not in normal tissues, are consistent with the proposal that Fhit inactivation plays a role in oral carcinogenesis.
Primary chronic osteomyelitis associated with synovitis, acne, pustulosis, hyperostosis and osteitis (SAPHO syndrome)
Authors: Eyrich G. K. H. , Harder C. , Sailer H. F. , Langenegger T. , Bruder E. , Michel B. A. 
Published: August 2010

Eyrich GKH, Harder C, Sailer HF, Langenegger T, Bruder E, Michel BA: Primary chronic osteomyelitis associated with synovitis, acne, pustulosis, hyperostosis and osteitis (SAPHO syndrome). J Oral Pathol Med 1999; 28: 456–64. © Munksgaard, 1999.Diffuse sclerosing osteomyelitis may indicate the mandibular localisation of the SAPHO syndrome. Twelve patients with diffuse sclerosis of the mandible were examined for symptoms of the SAPHO syndrome. Nine patients were found to have primary chronic osteomyelitis and eight of these represented a SAPHO syndrome. Results in this series support the hypothesis of an association between primary chronic osteomyelitis and the SAPHO syndrome.
Collagenous fibroma (desmoplastic fibroblastoma) presenting as a parotid mass
Authors: Ide Fumio , Shimoyama Tetsuo , Horie Norio , Tanaka Hiroshi 
Published: August 2010

Ide F, Shimoyama T, Horie N, Tanaka H: Collagenous fibroma (desmoplastic fibroblastoma) presenting as a parotid mass. J Oral Pathol Med 1999; 28: 465–8. © Munksgaard, 1999.We describe the case of a 50‐year‐old man who was found to have a painless, slow‐growing parotid mass of 10 months duration. At surgery, a well‐delimited, lobulated 4 times 5 cm tumor was located on the deep lobe of the left parotid gland, adhering to the parotid sheath and masseteric fascia. Follow‐up 6 years later has shown no evidence of recurrence. Cardinal morphologic features included multiple nodules of sparsely distributed stellate‐or spindle‐shaped fibroblasts within a collagenous or myxocollagenous stroma. Fascial involvement and entrapment of salivary gland were focally identified at the edges. Tumor cells were diffusely positive for vimentin with faint focal staining for α‐smooth muscle actin. These findings satisfied the diagnostic criteria for collagenous fibroma (desmoplastic fibroblastoma). Ours represents the first report of this tumor type mimicking a parotid tumor.
The molecular markers for prognostic evaluation of areca‐associated buccal squamous cell carcinoma
Authors: Liu Chung‐Ji , Chang Kuo‐Wei , Chao Shou‐Yee , Kwan Po‐Cheung , Chang Shun‐Min , Yen Rui‐Yin , Wang Chun‐Yu , Wong Yong‐Kie 
Published: August 2010

Background:  Buccal squamous cell carcinoma (BSCC) is the most frequently occurring oral cancer in Asians due to the popularity of areca use in this area. The aim of the present study was to evaluate the survival of areca‐associated BSCC associated with multiple molecular markers.Methods:  Using immunohistochemistry, we evaluated the survival of a cohort of 55 patients with BSCC being followed long term, as correlated to the expression of variable markers.Results:  We found that p53, p21, Rb, cyclin D1 (CCD1), MDM2, and γ‐catenin were positive in 81, 60, 70, 31, 88, and 44% of patients, respectively. Subjects with −ve immunoreactivity for CCD1, and +ve immunoreactivity for MDM2 and γ‐catenin had significantly better survival than subjects with the opposite immunoreactive pattern. Kaplan–Meier survival curves confirmed this association.Conclusion:  The data indicate that expression of CCD1, MDM2, and γ‐catenin might serve as potential prognostic markers for BSCC in areca‐using patients.
Relationship between major and minor salivary gland mucoepidermoid carcinoma malignancy grading and presence of stromal myofibroblasts: immunohistochemical study
Authors: Sobral Ana Paula Veras , Loducca Silvia Vanessa L. , Nunes Fábio Daumas , De Araújo Ney Soares , Kowalski Luiz Paulo , De Araújo Vera Cavalcanti 
Published: August 2010

Objective:  Mucoepidermoid carcinoma (MEC) is the most common malignant salivary tumour, classified as low, intermediate and high grade. Myofibroblasts are the main stromal component and are included as prognostic factor in some tumours. The aim of this study was to evaluate the myofibroblasts in the stroma of MEC with possible relationship to malignancy grading.Methods:  Twenty‐five cases of MEC (six low grade, 11 intermediate grade, four high grade and four metastasis) were stained for vimentin, desmin and smooth muscle actin (SMA) for the identification of myofibroblasts. Transforming growth factors (TGFβ1 and TGFβRII) were also assessed in our study.Results:  Myofibroblasts were present in all cases, in amounts varying according to histological grading. TGFβ1 was positive in squamous cells of intermediate grade tumours, and in the stroma of only four cases. TGFβRII was positive in most squamous and intermediate cells, regardless of malignancy grading.Conclusions:  Our study showed that the analysis of neoplastic stroma must be added to the studies of neoplastic cells to draw a better picture leading to tumour diagnosis and prognosis.
Treatment of gingival lichen with free palatal grafts
Authors: Axéll Tony , Henriksen Birgitte Moesgaard 
Published: August 2010

Background:  Recalcitrant gingival erythematous lichen planus/lichenoid lesions comprise a considerable therapeutic problem. The objective of this study was to evaluate the therapeutic effect of grafting keratinized oral palatal mucosa to the sites of gingival lichen.Methods:  In 12 patients 20 grafts were transplanted to buccal gingival lesions. Mean age of the patients was 59.8 ± 7.1 years (range 46–71 years). The mean observation time was 32 ± 32.7 months (range 5–97 months).Results:  On a 4‐point clinical grade scale (0–3), 12 (60%) transplants showed complete healing grade 3, six (30%) grade 2 and two (10%) grade 1.Conclusions:  Using oral mucosal grafts from the palatal mucosa for the treatment of recalcitrant erythematous gingival lichen planus/lichenoid lesions seems to be a promising treatment modality.
Detection of antibodies against p63 and p73 isoforms in sera from patients diagnosed with oral lichen planus
Authors: Ebrahimi Majid , Wahlin Ylva‐Britt , Coates Philip J. , Wiik Allan , Roos Göran , Nylander Karin 
Published: August 2010

Background:  Oral lichen planus (OLP) is a chronic inflammatory disease of oral mucosa. Despite numerous publications and intense research, the etiology of OLP is still unknown, however, autoimmunity as a possible causative factor has been discussed.Methods:  In the present study sera from 20 patients clinically and histologically diagnosed with OLP were analyzed for antibodies directed toward p53, p63, and p73 using Western blot.Results:  Sera from two patients reacted with all six p63 isoforms, and one also with p73. The strongest reaction was noted against the TAp63β protein, which is the most potent transactivator of all p63 proteins and is implicated in the differentiation of stratified epithelia.Conclusions:  This is the first demonstration of antibodies directed against all p63 and some p73 isoforms in sera from patients diagnosed with OLP.
Oral and pharyngeal cancer mortality rates in Mexico, 1979–2003
Authors: Anaya‐Saavedra Gabriela , Ramírez‐Amador Velia , Irigoyen‐Camacho María Esther , Zimbrón‐Romero Araceli , Zepeda‐Zepeda Marco Antonio 
Published: August 2010

Background:  In Mexico, information on oral and pharyngeal cancer (OPC) is scarce. The purpose of this study was to explore the trends in OPC mortality rates in Mexico from 1979 through 2003 and to describe the distribution of OPC deaths for selected socio‐demographic variables for the period of 2001–2003.Material and methods:  Annual crude and age‐adjusted mortality rates were obtained by gender and site of lesion, using the 2003 WHO World standard million population. The Poisson regression model was used to detect a trend in the mortality rates, testing the hypothesis β1 = 0. Also, the annual percentage change (APC) was computed over the age‐adjusted rates.Results:  The total number of OPC deaths during the period 1979–2003 was 15 576. The age‐adjusted mortality rate was 1.13/100 000 in 1979 and 1.08/100 000 in 2003. Oral cancer was more frequently found than salivary gland and pharyngeal cancer (41.5% vs. 13.4% and 17.1%). The tongue (19%) was the most frequent oral affected site. The Poisson regression analysis indicated a stationary trend in cancer mortality rate; also, the APC regression model showed no increase or decrease in OPC from 1979 to 2003.Conclusions:  Oral and pharyngeal cancer mortality rates in Mexico were low compared to most countries, and remained stable in the past two decades.