bluuebunny/crossref_metadata_2025_split huggingface dataset:
Techno-economics of multi-stage reverse electrodialysis for blue energy harvesting
Authors: Wang Lu , Zhao Yanan , Long Rui , Liu Zhichun , Liu Wei 
Published: May 2024

AbstractMulti-stage reverse electrodialysis (MSRED) offers a promising way for efficient salinity gradient energy harvesting. Here, an improved model of the MSRED system under serial control strategy is proposed. The technical–economic analysis is conducted with considering discount, depreciation and different regional tax and electricity price levels under the maximum net power output conditions. Results reveal that net power output and energy efficiency both increase first with increasing stage numbers, reach their maximum values, and then decrease. For 5 M/0.05 M solutions, the optimal net power output of 4.98 kW is obtained at the stage number n = 12. The optimal stage number corresponding to the maximum net power increases with increasing feed solution concentrations. Due to the compromise between net power generation and capital cost, there exist optimal stage numbers leading to the lowest LCOE and largest NPV, respectively. Higher feed solution concentration can significantly decrease the system LCOE and increase the NPV. The optimal stage number corresponding to the maximum NPV increases with increasing feed solution concentrations. In Germany, for 5 M/0.05 M solutions, the lowest LCOE of 0.061 €·kWh−1 is achieved at n = 3 while the highest NPV over the system lifecycle of 52,005 € is obtained at n = 8. Lower tax, higher electricity price, appropriate membrane price and stage numbers, and high salinity gradient sources can significantly accelerate the commercial completeness of the MSRED systems.
Otoendoscopy as a better preoperative predictor than pure tone audiometry for ossicular erosion in mucosal chronic otitis media
Authors: Bhardwaj Abhishek , Gupta Kartikesh , PS Suji , Malhotra Manu , Priya Madhu , Varshney Saurabh , Sood Rachit , Singh Arpana 
Published: May 2024

AbstractObjectiveTo compare the diagnostic accuracy of angled otoendoscopy with pure tone audiometry in predicting ossicular discontinuity in patients of mucosal chronic otitis media.MethodsNinety-four patients were included in this prospective study. A 2.7-mm 30° otoendoscope was used to examine ossicular status preoperatively. Hearing thresholds were recorded by pure tone audiometry. Intraoperative ossicular status was recorded as the gold standard. Otoendoscopic findings were recorded as per the criteria has been devised by the authors of this manuscript.ResultsOtoendoscopy was conclusive in 56 (59.6 per cent) patients, with 100 per cent sensitivity, 95.56 per cent specificity, 84.62 per cent positive predictive value, and 100 per cent negative predictive value in the conclusive group. Overall (in 94 patients), diagnostic test values of otoendoscopy were 73.33 per cent sensitivity, 97.47 per cent specificity, 84.62 per cent positive predictive value, and 95.06 per cent negative predictive value. As per the ROC curve, air–bone gap > 38.12dB had the optimal diagnostic test values, with 73 per cent sensitivity, 72 per cent specificity, 33.3 per cent positive predictive value, and 93.4 per cent negative predictive value.ConclusionAngled otoendoscopy has better diagnostic accuracy (93.6 per cent) than pure tone audiometry (72.3 per cent; p < 0.001) for preoperative ossicular discontinuity prediction in patients of mucosal chronic otitis media.
Neutrophils and neutrophil extracellular traps in oral health and disease
Authors: Kim Tae Sung , Moutsopoulos Niki M. 
Published: May 2024

Abstract
Neutrophils perform essential functions in antimicrobial defense and tissue maintenance at mucosal barriers. However, a dysregulated neutrophil response and, in particular, the excessive release of neutrophil extracellular traps (NETs) are implicated in the pathology of various diseases. In this review, we provide an overview of the basic concepts related to neutrophil functions, including NET formation, and discuss the mechanisms associated with NET activation and function in the context of the prevalent oral disease periodontitis.
Targeting pyruvate dehydrogenase kinase 1 overcomes EGFR C797S mutation-driven osimertinib resistance in non-small cell lung cancer
Authors: Park Wonyoung , Wei Shibo , Xie Chu-Long , Han Jung Ho , Kim Bo-Sung , Kim Bosung , Jin Jung-Sook , Yang Eun-Sun , Cho Min Kyoung , Ryu Dongryeol , Yang Hao-Xian , Bae Sung-Jin , Ha Ki-Tae 
Published: May 2024

AbstractOsimertinib, a selective third-generation epidermal growth factor receptor (EGFR) tyrosine kinase inhibitor (TKI), effectively targets the EGFR T790M mutant in non-small cell lung cancer (NSCLC). However, the newly identified EGFR C797S mutation confers resistance to osimertinib. In this study, we explored the role of pyruvate dehydrogenase kinase 1 (PDK1) in osimertinib resistance. Patients exhibiting osimertinib resistance initially displayed elevated PDK1 expression. Osimertinib-resistant cell lines with the EGFR C797S mutation were established using A549, NCI-H292, PC-9, and NCI-H1975 NSCLC cells for both in vitro and in vivo investigations. These EGFR C797S mutant cells exhibited heightened phosphorylation of EGFR, leading to the activation of downstream oncogenic pathways. The EGFR C797S mutation appeared to increase PDK1-driven glycolysis through the EGFR/AKT/HIF-1α axis. Combining osimertinib with the PDK1 inhibitor leelamine helped successfully overcome osimertinib resistance in allograft models. CRISPR-mediated PDK1 knockout effectively inhibited tumor formation in xenograft models. Our study established a clear link between the EGFR C797S mutation and elevated PDK1 expression, opening new avenues for the discovery of targeted therapies and improving our understanding of the roles of EGFR mutations in cancer progression.
Adult attitudes to sustainable dentistry in Trinidad and Tobago and their willingness to accept alternatives
Authors: Hoyte Trudee , James Akini , Carr Deysha , Teelucksingh Abbinah Donatien Andrew , Mossey Peter 
Published: May 2024

Abstract
Introduction
Attitudes towards and willingness to accept alternatives for sustainable dentistry in Trinidad and Tobago have never been assessed. Market research aids in the understanding of the behaviours of people. Since change can be enacted by public pressure, it is worth engaging the public through research to understand their attitudes and which changes they are willing to accept.

Method
A self-administered questionnaire was distributed to private and public dental clinics. The questionnaire assessed attitude towards and willingness to accept alternatives which decrease the effect of dental treatment on the environment.

Results
The study consisted of 1267 participants. Participants were mostly female, older, employed and mainly of African descent. Participants reported a very positive attitude towards sustainable dentistry (Mean = 3.89, SD = 0.8). and were moderately willing to accept alternatives such as a longer appointment time (Mean 3.47, SD = 0.73) and pay more for their dental treatments (Mean=3.00, SD = 0.87). There was a strong positive correlation with attitudes to sustainable dentistry and participants willingness to accept alternatives such as a longer appointment time (r = 0.658, p < 0.05).

Conclusion
The adult population had an overall positive attitude towards sustainable dentistry and was willing to accept alternatives so that their dental treatment would have less impact on the environment.
Actin-nucleation promoting factor N-WASP influences alpha-synuclein condensates and pathology
Authors: Jackson Joshua , Hoffmann Christian , Scifo Enzo , Wang Han , Wischhof Lena , Piazzesi Antonia , Mondal Mrityunjoy , Shields Hanna , Zhou Xuesi , Mondin Magali , Ryan Eanna B. , Döring Hermann , Prehn Jochen H. M. , Rottner Klemens , Giannone Gregory , Nicotera Pierluigi , Ehninger Dan , Milovanovic Dragomir , Bano Daniele 
Published: May 2024

AbstractAbnormal intraneuronal accumulation of soluble and insoluble α-synuclein (α-Syn) is one of the main pathological hallmarks of synucleinopathies, such as Parkinson’s disease (PD). It has been well documented that the reversible liquid-liquid phase separation of α-Syn can modulate synaptic vesicle condensates at the presynaptic terminals. However, α-Syn can also form liquid-like droplets that may convert into amyloid-enriched hydrogels or fibrillar polymorphs under stressful conditions. To advance our understanding on the mechanisms underlying α-Syn phase transition, we employed a series of unbiased proteomic analyses and found that actin and actin regulators are part of the α-Syn interactome. We focused on Neural Wiskott-Aldrich syndrome protein (N-WASP) because of its association with a rare early-onset familial form of PD. In cultured cells, we demonstrate that N-WASP undergoes phase separation and can be recruited to synapsin 1 liquid-like droplets, whereas it is excluded from α-Syn/synapsin 1 condensates. Consistently, we provide evidence thatwsp-1/WASL loss of functionalters the number and dynamics of α-Syn inclusions in the nematodeCaenorhabditis elegans. Together, our findings indicate that N-WASP expression may create permissive conditions that promote α-Syn condensates and their potentially deleterious conversion into toxic species.
Impact of the thyroid hormone T3 and its nuclear receptor TRα1 on colon cancer stem cell phenotypes and response to chemotherapies
Authors: Giolito Maria Virginia , Bodoirat Serguei , La Rosa Theo , Reslinger Mathieu , Guardia Gabriela D. A. , Mourtada Jana , Claret Leo , Joung Alain , Galante Pedro A. F. , Penalva Luiz O. F. , Plateroti Michelina 
Published: May 2024

AbstractColorectal cancers (CRCs) are highly heterogeneous and show a hierarchical organization, with cancer stem cells (CSCs) responsible for tumor development, maintenance, and drug resistance. Our previous studies showed the importance of thyroid hormone-dependent signaling on intestinal tumor development and progression through action on stem cells. These results have a translational value, given that the thyroid hormone nuclear receptor TRα1 is upregulated in human CRCs, including in the molecular subtypes associated with CSC features. We used an established spheroid model generated from the human colon adenocarcinoma cell line Caco2 to study the effects of T3 and TRα1 on spheroid formation, growth, and response to conventional chemotherapies. Our results show that T3 treatment and/or increased TRα1 expression in spheroids impaired the response to FOLFIRI and conferred a survival advantage. This was achieved by stimulating drug detoxification pathways and increasing ALDH1A1-expressing cells, including CSCs, within spheroids. These results suggest that clinical evaluation of the thyroid axis and assessing TRα1 levels in CRCs could help to select optimal therapeutic regimens for patients with CRC.
Modeling acute myocardial infarction and cardiac fibrosis using human induced pluripotent stem cell-derived multi-cellular heart organoids
Authors: Song Myeongjin , Choi Da Bin , Im Jeong Suk , Song Ye Na , Kim Ji Hyun , Lee Hanbyeol , An Jieun , Kim Ami , Choi Hwan , Kim Joon-Chul , Han Choongseong , Jeon Young Keul , Kim Sung Joon , Woo Dong-Hun 
Published: May 2024

AbstractHeart disease involves irreversible myocardial injury that leads to high morbidity and mortality rates. Numerous cell-based cardiac in vitro models have been proposed as complementary approaches to non-clinical animal research. However, most of these approaches struggle to accurately replicate adult human heart conditions, such as myocardial infarction and ventricular remodeling pathology. The intricate interplay between various cell types within the adult heart, including cardiomyocytes, fibroblasts, and endothelial cells, contributes to the complexity of most heart diseases. Consequently, the mechanisms behind heart disease induction cannot be attributed to a single-cell type. Thus, the use of multi-cellular models becomes essential for creating clinically relevant in vitro cell models. This study focuses on generating self-organizing heart organoids (HOs) using human-induced pluripotent stem cells (hiPSCs). These organoids consist of cardiomyocytes, fibroblasts, and endothelial cells, mimicking the cellular composition of the human heart. The multi-cellular composition of HOs was confirmed through various techniques, including immunohistochemistry, flow cytometry, q-PCR, and single-cell RNA sequencing. Subsequently, HOs were subjected to hypoxia-induced ischemia and ischemia-reperfusion (IR) injuries within controlled culture conditions. The resulting phenotypes resembled those of acute myocardial infarction (AMI), characterized by cardiac cell death, biomarker secretion, functional deficits, alterations in calcium ion handling, and changes in beating properties. Additionally, the HOs subjected to IR efficiently exhibited cardiac fibrosis, displaying collagen deposition, disrupted calcium ion handling, and electrophysiological anomalies that emulate heart disease. These findings hold significant implications for the advancement of in vivo-like 3D heart and disease modeling. These disease models present a promising alternative to animal experimentation for studying cardiac diseases, and they also serve as a platform for drug screening to identify potential therapeutic targets.
TM7SF2-induced lipid reprogramming promotes cell proliferation and migration via CPT1A/Wnt/β-Catenin axis in cervical cancer cells
Authors: Liu Hejing , Liu Yi , Zhou Yujia , Chen Xin , Pan Shuya , Zhou Qingfeng , Ji Huihui , Zhu Xueqiong 
Published: May 2024

AbstractCervical cancer poses a serious threat to women’s health globally. Our previous studies found that upregulation of TM7SF2, which works as an enzyme involved in the process of cholesterol biosynthesis expression, was highly correlated with cervical cancer. However, the mechanistic basis of TM7SF2 promoting cervical cancer progression via lipid metabolism remains poorly understood. Therefore, quantification of fatty acids and lipid droplets were performed in vitro and in vivo. The protein-protein interaction was verified by Co-IP technique. The mechanism and underlying signaling pathway of TM7SF2 via CPT1A associated lipid metabolism in cervical cancer development were explored using Western blotting, IHC, colony formation, transwell assay, and wound healing assay. This study reported that overexpression of TM7SF2 increased fatty acids content and lipid droplets both in vivo and in vitro experiments. While knockout of TM7SF2 obviously attenuated this process. Moreover, TM7SF2 directly bonded with CPT1A, a key enzyme in fatty acid oxidation, and regulated CPT1A protein expression in cervical cancer cells. Notably, the proliferation and metastasis of cervical cancer cells were elevated when their CPT1A expression was upregulated. Then, rescue assay identified that CPT1A overexpressed could enhance the cell viability and migration in TM7SF2-knockout cells. Furthermore, depletion of TM7SF2 significantly inhibited WNT and β-catenin proteins expression, which was enhanced by CPT1A-overexpressed. The proliferation and migration of cervical cancer cells were reversed in CPT1A-overexpressed cells with the treatment of MSAB, an inhibitor of Wnt/β-Catenin pathway. This study put forward an idea that TM7SF2-induced lipid reprogramming promotes proliferation and migration via CPT1A/Wnt/β-Catenin axis in cervical cancer, underlying the progression of cervical cancer.
LARP4B promotes hepatocellular carcinoma progression and impairs sorafenib efficacy by activating SPINK1-mediated EGFR pathway
Authors: Wang Chuanxu , Dong Rui , Yang Feicheng , Zheng Lu , Liu Yingling , Yan Yue , Zhang Mengjie , Ni Bing , Li Jing 
Published: May 2024

AbstractLa-related proteins (LARPs) regulate gene expression by binding to RNAs and exhibit critical effects on disease progression, including tumors. However, the role of LARP4B and its underlying mechanisms in the progression of hepatocellular carcinoma (HCC) remain largely unclear. In this study, we found that LARP4B expression is upregulated and correlates with poor prognosis in patients with HCC. Gain- and loss-of-function assays showed that LARP4B promotes stemness, proliferation, metastasis, and angiogenesis in vitro and in vivo. Furthermore, LARP4B inhibition enhances the antitumor effects of sorafenib and blocks the metastasis-enhancing effects of low sorafenib concentrations in HCC. Mechanistically, LARP4B expression is upregulated by METTL3-mediated N6-methyladenosine (m6A)-IGF2BP3-dependent modification in HCC. RNA- and RNA immunoprecipitation (RIP)- sequencing uncovered that LARP4B upregulates SPINK1 by binding to SPINK1 mRNA via the La motif and maintaining mRNA stability. LARP4B activates the SPINK1-mediated EGFR signaling pathway, which supports stemness, progression and sorafenib resistance in HCC. Additionally, a positive feedback loop with the LARP4B/SPINK1/p-AKT/C/EBP-β axis is responsible for the sorafenib-therapeutic benefit of LARP4B depletion. Overall, this study demonstrated that LARP4B facilitates HCC progression, and LARP4B inhibition provides benefits to sorafenib treatment in HCC, suggesting that LARP4B might be a potential therapeutic target for HCC.
Training an Ising machine with equilibrium propagation
Authors: Laydevant Jérémie , Marković Danijela , Grollier Julie 
Published: May 2024

AbstractIsing machines, which are hardware implementations of the Ising model of coupled spins, have been influential in the development of unsupervised learning algorithms at the origins of Artificial Intelligence (AI). However, their application to AI has been limited due to the complexities in matching supervised training methods with Ising machine physics, even though these methods are essential for achieving high accuracy. In this study, we demonstrate an efficient approach to train Ising machines in a supervised way through the Equilibrium Propagation algorithm, achieving comparable results to software-based implementations. We employ the quantum annealing procedure of the D-Wave Ising machine to train a fully-connected neural network on the MNIST dataset. Furthermore, we demonstrate that the machine’s connectivity supports convolution operations, enabling the training of a compact convolutional network with minimal spins per neuron. Our findings establish Ising machines as a promising trainable hardware platform for AI, with the potential to enhance machine learning applications.
Charge density waves tuned by biaxial tensile stress
Authors: Gallo–Frantz A. , Jacques V. L. R. , Sinchenko A. A. , Ghoneim D. , Ortega L. , Godard P. , Renault P.-O. , Hadj-Azzem A. , Lorenzo J. E. , Monceau P. , Thiaudière D. , Grigoriev P. D. , Bellec E. , Le Bolloc’h D. 
Published: May 2024

AbstractThe precise arrangement and nature of atoms drive electronic phase transitions in condensed matter. To explore this tenuous link, we developed a true biaxial mechanical deformation device working at cryogenic temperatures, compatible with x-ray diffraction and transport measurements, well adapted to layered samples. Here we show that a slight deformation of TbTe3 can have a dramatic influence on its Charge Density Wave (CDW), with an orientational transition from c to a driven by the a/c parameter, a tiny coexistence region near a = c, and without space group change. The CDW transition temperature Tc displays a linear dependence with $$\left\vert a/c-1\right\vert$$

a
/
c
−
1

while the gap saturates out of the coexistence region. This behaviour is well accounted for within a tight-binding model. Our results question the relationship between gap and Tc in RTe3 systems. This method opens a new route towards the study of coexisting or competing electronic orders in condensed matter.
Unraveling the mechanisms of PAMless DNA interrogation by SpRY-Cas9
Authors: Hibshman Grace N. , Bravo Jack P. K. , Hooper Matthew M. , Dangerfield Tyler L. , Zhang Hongshan , Finkelstein Ilya J. , Johnson Kenneth A. , Taylor David W. 
Published: May 2024

AbstractCRISPR-Cas9 is a powerful tool for genome editing, but the strict requirement for an NGG protospacer-adjacent motif (PAM) sequence immediately next to the DNA target limits the number of editable genes. Recently developed Cas9 variants have been engineered with relaxed PAM requirements, including SpG-Cas9 (SpG) and the nearly PAM-less SpRY-Cas9 (SpRY). However, the molecular mechanisms of how SpRY recognizes all potential PAM sequences remains unclear. Here, we combine structural and biochemical approaches to determine how SpRY interrogates DNA and recognizes target sites. Divergent PAM sequences can be accommodated through conformational flexibility within the PAM-interacting region, which facilitates tight binding to off-target DNA sequences. Nuclease activation occurs ~1000-fold slower than for Streptococcus pyogenes Cas9, enabling us to directly visualize multiple on-pathway intermediate states. Experiments with SpG position it as an intermediate enzyme between Cas9 and SpRY. Our findings shed light on the molecular mechanisms of PAMless genome editing.
Noncovalent synthesis of homo and hetero-architectures of supramolecular polymers via secondary nucleation
Authors: Kotha Srinu , Sahu Rahul , Yadav Aditya Chandrakant , Sharma Preeti , Kumar B. V. V. S. Pavan , Reddy Sandeep K. , Rao Kotagiri Venkata 
Published: May 2024

AbstractThe synthesis of supramolecular polymers with controlled architecture is a grand challenge in supramolecular chemistry. Although living supramolecular polymerization via primary nucleation has been extensively studied for controlling the supramolecular polymerization of small molecules, the resulting supramolecular polymers have typically exhibited one-dimensional morphology. In this report, we present the synthesis of intriguing supramolecular polymer architectures through a secondary nucleation event, a mechanism well-established in protein aggregation and the crystallization of small molecules. To achieve this, we choose perylene diimide with 2-ethylhexyl chains at the imide position as they are capable of forming dormant monomers in solution. Activating these dormant monomers via mechanical stimuli and hetero-seeding using propoxyethyl perylene diimide seeds, secondary nucleation event takes over, leading to the formation of three-dimensional spherical spherulites and scarf-like supramolecular polymer heterostructures, respectively. Therefore, the results presented in this study propose a simple molecular design for synthesizing well-defined supramolecular polymer architectures via secondary nucleation.
Waveguide-integrated twisted bilayer graphene photodetectors
Authors: Wu Qinci , Qian Jun , Wang Yuechen , Xing Luwen , Wei Ziyi , Gao Xin , Li Yurui , Liu Zhongfan , Liu Hongtao , Shu Haowen , Yin Jianbo , Wang Xingjun , Peng Hailin 
Published: May 2024

AbstractGraphene photodetectors have exhibited high bandwidth and capability of being integrated with silicon photonics (SiPh), holding promise for future optical communication devices. However, they usually suffer from a low photoresponsivity due to weak optical absorption. In this work, we have implemented SiPh-integrated twisted bilayer graphene (tBLG) detectors and reported a responsivity of 0.65 A W–1 for telecom wavelength 1,550 nm. The high responsivity enables a 3-dB bandwidth of >65 GHz and a high data stream rate of 50 Gbit s–1. Such high responsivity is attributed to the enhanced optical absorption, which is facilitated by van Hove singularities in the band structure of high-mobility tBLG with 4.1o twist angle. The uniform performance of the fabricated photodetector arrays demonstrates a fascinating prospect of large-area tBLG as a material candidate for heterogeneous integration with SiPh.