bluuebunny/crossref_metadata_2025_split huggingface dataset:
Evaluation of NDRG2 gene expression in primary papillary thyroid carcinoma and in metastases of this neoplasm to regional lymph nodes
Authors: Mordalska Anna , Latek Joanna , Ferenc Tomasz , Pomorski Lech , Gałecka Elżbieta , Zygmunt Arkadiusz , Lewiński Andrzej 
Published: August 2010

Abstract

Background
At present, researchers' attention has been concentrating on NDRG2 (N-Myc downstream-regulated gene 2) as a new gene candidate in the development and progression of papillary thyroid carcinoma (PTC). NDRG2, together with NDRG1, NDRG3 and NDRG4 are members of the NDRG family, a new class of genes, inhibited by N-Myc oncogene.

Aim
The aim of our study was to evaluate NDRG2 mRNA expression in the primary PTC and its metastases to regional lymph nodes.

Materials and methods
Postoperative tissue and macroscopically changed lymph nodes of sixteen (16) patients with PTC constituted the studied material. In this group, metastases of the cancer to regional lymph nodes were confirmed histopathologically in 8 cases. Quantitative evaluation of NDRG2 mRNA expression was performed by the real-time polymerase chain reaction (real-time PCR) method.

Results
The mean values of NDRG2 mRNA expression in the primary tumour tissues were statistically significantly lower vs. the levels of NDRG2 mRNA expression in macroscopically unchanged thyroid tissue (p < 0.0001). A comparison of the mean NDRG2 mRNA expression of primary tumours and that of their metastases to regional lymph nodes did not demonstrate any statistical differences (p > 0.05). A positive correlation was observed between NDRG2 mRNA expression in primary tumour cells and in the cancer metastases to lymph nodes (Rs = 0.7857; p < 0.05). Factors, such as age, sex, tumour stage in TNM system, were of no significance for NDRG2 mRNA expression level (p > 0.1).

Conclusion
The results of our study demonstrated decreased NDRG2 mRNA expression levels in PTC, when compared to macroscopically unchanged thyroid tissue, which may point to the potential role of NDRG2 in the development and progression of cancer in question.
Personality disorder and the Mental Health Act 1983 (amended)
Authors: Sen Piyal , Irons Ashley 
Published: August 2010

SummaryThe Mental Health Act 1983 now incorporates amendments introduced in 2007. This article explores features of the amended Act that affect the treatment of patients with personality disorder in England and Wales. It discusses issues such as the broad definition of mental disorder, treatability and professional roles, with specific reference to how they might, or might not, affect usual practice concerning patients with personality disorder. It also comments on elements within the Act that could positively affect people with personality disorder, such as community treatment orders, provision to change their ‘nearest relative’ and statutory advocacy services. The political climate in which the Act has been amended is commented on, as well as how this might potentially compromise some of the positives within the Act.
Parental history of depression or anxiety and the cortisol awakening response
Authors: Vreeburg Sophie A. , Hartman Catharina A. , Hoogendijk Witte J. G. , Dyck Richard van , Zitman Frans G. , Ormel Johan , Penninx Brenda W. J. H. 
Published: August 2010

BackgroundIt is unclear whether altered hypothalamic–pituitary–adrenal (HPA) axis regulation, which frequently accompanies depression and anxiety disorders, represents a trait rather than a state factor.AimsTo examine whether HPA axis dysregulation represents a biological vulnerability for these disorders, we compared cortisol levels in unaffected people with and without a parental history of depressive or anxiety disorders. We additionally examined whether possible HPA axis dysregulations resemble those observed in participants with depression or anxiety disorders.MethodData were from the Netherlands Study of Depression and Anxiety. Within the participants without a lifetime diagnoses of depression or anxiety disorders, three groups were distinguished: 180 people without parental history, 114 with self-reported parental history and 74 with CIDI-diagnosed parental history. These groups were additionally compared with people with major depressive disorder or panic disorder with agoraphobia (n = 1262). Salivary cortisol samples were obtained upon awakening, and 30, 45 and 60 min later.ResultsAs compared with unaffected participants without parental history, unaffected individuals with diagnosed parental history of depression or anxiety showed a significantly higher cortisol awakening curve (effect size (d) = 0.50), which was similar to that observed in the participants with depression or anxiety disorders. Unaffected people with self-reported parental history did not differ in awakening cortisol levels from unaffected people without parental history.ConclusionsUnaffected individuals with parental history of depression or anxiety showed a higher cortisol awakening curve, similar to that of the participants with depression or anxiety disorders. This suggests that a higher cortisol awakening curve reflects a trait marker, indicating an underlying biological vulnerability for the development of depressive and anxiety disorders.
Personality pathology recorded by severity: national survey
Authors: Yang Min , Coid Jeremy , Tyrer Peter 
Published: August 2010

BackgroundCurrent classifications of personality disorders do not classify severity despite clinical practice favouring such descriptions.AimsTo assess whether an existing measure of severity of personality disorder predicted clinical pathology and societal dysfunction in a community sample.MethodUK national epidemiological study in which personality status was measured using the screening version of the Structured Clinical Interview for DSM–IV Personality Disorders (SCID–II) and reclassified to five levels using a modified severity index. Associations between levels of severity of personality pathology and social, demographic and clinical variables were measured.ResultsOf 8391 individuals interviewed and their personality status assessed, only a minority (n = 1933, 23%) had no personality pathology. The results supported the hypothesis. More severe personality pathology was associated incrementally with younger age, childhood institutional care, expulsion from school, contacts with the criminal justice system, economic inactivity, more Axis I pathology and greater service contact (primary care and secondary care, allP<0.001). Significant handicap was noted among people with even low levels of personality pathology. No differences contradicted the main hypothesis.ConclusionsA simple reconstruction of the existing classification of personality disorder is a good predictor of social dysfunction and supports the development of severity measures as a critical requirement in both DSM–V and ICD–11 classifications.
Facilities for carers of in-patients in forensic psychiatric services in England and Wales
Authors: Cormac Irene , Lindon Drew , Jones Hannah , Gedeon Trevor , Ferriter Michael 
Published: August 2010

Aims and methodA postal survey of forensic psychiatric facilities in England and Wales was undertaken to obtain information about the services provided for carers of in-patients within these services.ResultsForensic psychiatric services vary in the support and facilities provided for carers. Many do not comply with current legislation for carers. Most units informed carers of their rights to have an assessment, but only a minority provided facilities for carers from Black and minority ethnic backgrounds.Clinical implicationsForensic psychiatric services should meet standards for the involvement and support of carers in mental health settings, and comply with legislation for carers.
Information and readability issues for psychiatric patients: e-learning for users
Authors: Gralton Ernest , Sher Marilyn , Lopez Coro Drew 
Published: August 2010

Aims and methodThe level of reading ability required to understand written information about key mental health issues designed for service users is examined. Information was taken from four reputable internet sources and analysed for readability. The relevant literature in relation to psychiatric patients and literacy is reviewed and potential solutions are proposed.ResultsA considerable proportion of the available information has a reading age at or above 14 years. Some organisations appear better than others in providing information at a more appropriate level of reading ability.Clinical implicationsWritten information aimed at users of psychiatric services may not take into account that they are more likely to have impaired reading ability, even though they might not have an identified intellectual disability. Professionals who develop written materials can use tools in word processing software to assist with the appropriate development of these materials. Information technology could in the future provide information directed at users of psychiatric services that does not rely so heavily on written material.
Trainees' perception of the Annual Review of Competence Progression: 2-year survey
Authors: Vasudev Akshya , Vasudev Kamini , Thakkar Pratish 
Published: August 2010

Aims and methodThe Annual Review of Competence Progression (ARCP) is a process of checking and evaluating evidence of competency attainment collected by a trainee over the preceding year. There has been no study evaluating trainees' perception of this new process. Two cross-sectional electronic surveys were conducted a year apart in the north of England. Out of 92 psychiatry trainees 58 (63%) completed the survey in 2008; 60 (63%) out of 96 trainees completed the second round in 2009.ResultsOver the year there was a significant improvement in trainees' perception of the new process: 75% of respondents highlighted non-availability of a list of acceptable evidence for the portfolio in 2008, which reduced to 22% in 2009 (P <0.001). The percentage of trainees facing difficulty in accessing the electronic portal reduced from 73 to 28% (P <0.001). The trainees continued to express the need for explicit feedback at ARCP and improved training of the assessors in addition to other parameters.Clinical implicationsThe process of ARCP seems to have become robust over the year studied. There is scope for further refining of the process according to trainees' needs.
Prospective Evaluation of Allogeneic Hematopoietic Stem-Cell Transplantation From Matched Related and Matched Unrelated Donors in Younger Adults With High-Risk Acute Myeloid Leukemia: German-Austrian Trial AMLHD98A
Authors: Schlenk Richard F. , Döhner Konstanze , Mack Silja , Stoppel Michael , Király Franz , Götze Katharina , Hartmann Frank , Horst Heinz A. , Koller Elisabeth , Petzer Andreas , Grimminger Wolfgang , Kobbe Guido , Glasmacher Axel , Salwender Hans , Kirchen Heinz , Haase Detlef , Kremers Stephan , Matzdorff Axel , Benner Axel , Döhner Hartmut 
Published: August 2010

Purpose To assess the impact of allogeneic hematopoietic stem-cell transplantation (HSCT) from matched related donors (MRDs) and matched unrelated donors (MUDs) on outcome in high-risk patients with acute myeloid leukemia (AML) within a prospective multicenter treatment trial. Patients and Methods Between 1998 and 2004, 844 patients (median age, 48 years; range, 16 to 62 years) with AML were enrolled onto protocol AMLHD98A that included a risk-adapted treatment strategy. High risk was defined by the presence of unfavorable cytogenetics and/or by no response to induction therapy. Results Two hundred sixty-seven (32%) of 844 patients were assigned to the high-risk group. Of these 267 patients, 51 patients (19%) achieved complete remission but had adverse cytogenetics, and 216 patients (81%) had no response to induction therapy. Allogeneic HSCT was actually performed in 162 (61%) of 267 high-risk patients, after a median time of 147 days after diagnosis. Graft sources were as follows: MRD (n = 62), MUD (n = 89), haploidentical donor (n = 10), and cord blood (n = 1). The 5-year overall survival rates were 6.5% (95% CI, 3.1% to 13.6%) for patients (n = 105) not proceeding to HSCT and 25.1% (95% CI, 19.1% to 33.0%; from date of transplantation) for patients (n = 162) receiving HSCT. Multivariable analysis including allogeneic HSCT as a time-dependent covariable revealed that allogeneic HSCT significantly improved outcome; there was no difference in outcome between allogeneic HSCT from MRD and MUD. Conclusion Allogeneic HSCT in younger adults with high-risk AML has a significant beneficial impact on outcome, and allogeneic HSCT from MRD and MUD yields similar results.
Beyond statin therapy: a review of the management of residual risk in diabetes mellitus
Authors: Judge Eoin P , Phelan D , O'Shea Donal 
Published: August 2010

Summary Total cholesterol and low-density lipoprotein (LDL) cholesterol exhibit an independent, strong, continuous correlation with cardiovascular events. The effectiveness of hydroxy-3-methylglutaryl coenzyme A reductase inhibitors (statins) in the treatment and prevention of atherosclerosis is well-established. However, despite the lowering of LDL targets and the increased use of statins, patients with type 2 diabetes mellitus (DM) continue to experience a higher proportion of adverse coronary artery disease events. This is as a result of an atherogenic dyslipidaemia, characterized by low levels of high-density lipoprotein and elevated plasma triglyceride concentrations, often with high levels of cholesterol-rich remnant particles. This article will review dyslipidaemia and its role in DM, and will discuss available treatment modalities that address residual cardiovascular risk in this disease.
Extended Bayesian LASSO for Multiple Quantitative Trait Loci Mapping and Unobserved Phenotype Prediction
Authors: Mutshinda Crispin M , Sillanpää Mikko J 
Published: August 2010

AbstractThe Bayesian LASSO (BL) has been pointed out to be an effective approach to sparse model representation and successfully applied to quantitative trait loci (QTL) mapping and genomic breeding value (GBV) estimation using genome-wide dense sets of markers. However, the BL relies on a single parameter known as the regularization parameter to simultaneously control the overall model sparsity and the shrinkage of individual covariate effects. This may be idealistic when dealing with a large number of predictors whose effect sizes may differ by orders of magnitude. Here we propose the extended Bayesian LASSO (EBL) for QTL mapping and unobserved phenotype prediction, which introduces an additional level to the hierarchical specification of the BL to explicitly separate out these two model features. Compared to the adaptiveness of the BL, the EBL is “doubly adaptive” and thus, more robust to tuning. In simulations, the EBL outperformed the BL in regard to the accuracy of both effect size estimates and phenotypic value predictions, with comparable computational time. Moreover, the EBL proved to be less sensitive to tuning than the related Bayesian adaptive LASSO (BAL), which introduces locus-specific regularization parameters as well, but involves no mechanism for distinguishing between model sparsity and parameter shrinkage. Consequently, the EBL seems to point to a new direction for QTL mapping, phenotype prediction, and GBV estimation.
Mapping Environment-Specific Quantitative Trait Loci
Authors: Chen Xin , Zhao Fuping , Xu Shizhong 
Published: August 2010

Abstract
Environment-specific quantitative trait loci (QTL) refer to QTL that express differently in different environments, a phenomenon called QTL-by-environment (Q × E) interaction. Q × E interaction is a difficult problem extended from traditional QTL mapping. The mixture model maximum-likelihood method is commonly adopted for interval mapping of QTL, but the method is not optimal in handling QTL interacting with environments. We partitioned QTL effects into main and interaction effects. The main effects are represented by the means of QTL effects in all environments and the interaction effects are represented by the variances of the QTL effects across environments. We used the Markov chain Monte Carlo (MCMC) implemented Bayesian method to estimate both the main and the interaction effects. The residual error covariance matrix was modeled using the factor analytic covariance structure. A simulation study showed that the factor analytic structure is robust and can handle other structures as special cases. The method was also applied to Q × E interaction mapping for the yield trait of barley. Eight markers showed significant main effects and 18 markers showed significant Q × E interaction. The 18 interacting markers were distributed across all seven chromosomes of the entire genome. Only 1 marker had both the main and the Q × E interaction effects. Each of the other markers had either a main effect or a Q × E interaction effect but not both.
Social Support and its Correlation with Loneliness: A Cross-Cultural Study of Nepalese Older Adults
Authors: Chalise Hom Nath , Kai Ichiro , Saito Tami 
Published: August 2010

The aim of this study was to analyze the situation of social support exchange among elderly men and women and to study the cross-cultural validity of predictors of loneliness in two Nepalese castes/ethnicities of older adults. Data for this study were taken from a cross-sectional study of the elderly at least 60 years old living in one ward of Kathmandu City, conducted in 2005. Loneliness was measured using a three-item loneliness scale based on the University of California at Los Angeles (UCLA) Loneliness Scale. Sources of social support were classified as spouse, children living with their elders, children living apart, and friends/neighbors. Study findings indicate that the main social support exchange for the elderly was with adult children living together, spouse, and friends/neighbors. Results also show gender difference in social support. Significant variables of loneliness were social support received from spouse, social support provided to spouse, and children living together with both elderly parents. This finding shows there is a high degree of cross-cultural invariance in the predictor sources of social support on loneliness among two Nepalese castes/ethnicities older adults.
Health-Related Quality of Life and Mortality in a General and Elderly Population of Patients With Type 2 Diabetes (ZODIAC-18)
Authors: Landman Gijs W.D. , van Hateren Kornelis J.J. , Kleefstra Nanne , Groenier Klaas H. , Gans Rijk O.B. , Bilo Henk J.G. 
Published: August 2010

OBJECTIVE
Diabetes negatively impacts the health-related quality of life (HRQOL) of patients with type 2 diabetes. An earlier analysis showed HRQOL to be associated with mortality, which suggests that measuring HRQOL could have clinical implications. We studied the association between HRQOL and total and cardiovascular mortality in patients with type 2 diabetes during long-term follow-up and specifically focused on old age and sex differences.

RESEARCH DESIGN AND METHODS
HRQOL was measured in a prospectively followed cohort of 1,353 patients with type 2 diabetes using the RAND-36. Cox proportional hazard models were used to measure the independent effect of baseline HRQOL on mortality.

RESULTS
During a mean follow-up of 9.6 years, 570 (42%) patients died, 280 of whom died of cardiovascular disease (49%). The Physical Component Score (PCS) and the Mental Component Score (MCS) were inversely associated with total mortality, with hazard ratios of 0.988 (95% CI 0.983–0.993) and 0.990 (95% CI 0.985–0.995), respectively. A 10-point-higher score on the PCS and MCS decreased the risk for total mortality by 11 and 10%, respectively. An inverse relationship with mortality was also seen for men, women, and for patients aged &gt;75 years. Mental health was significantly related to mortality in men but not in women.

CONCLUSIONS
Lower physical and mental HRQOL was associated with a higher total mortality and cardiovascular mortality in patients with type 2 diabetes; this is also the case when studying men and women and the elderly separately. The dimension mental health, related to depression and anxiety, was only associated with mortality in men, not in women.
L-rhamnose and L-fucose suppress cancer growth in mice
Authors: Tomsik Pavel , Soukup Tomas , Cermakova Eva , Micuda Stanislav , Niang Mohamed , Sucha Lenka , Rezacova Martina 
Published: August 2010

Abstract
It is documented that deficient fucosylation may play an important role in the pathogenesis of cancer. Since the supplementation of L-fucose could restore fucosylation in both in vitro and in vivo conditions, our intent was to examine the effect of intraperitoneal administration of L-fucose and L-rhamnose (a similar deoxysaccharide) on tumour growth, mitotic activity and metastatic setting of a solid form of Ehrlich carcinoma as well as on the survival rate of tumour bearing mice. Both L-fucose and L-rhamnose exerted a significant suppressive effect on tumour growth (P<0.05). After 10 days of therapy, the greatest inhibition of tumour growth expressed as a percentage of controls was observed in L-rhamnose at a dose of 3 g/kg/day (by 62%) and L-fucose at a dose of 5 g/kg/day (by 47%). Moreover, the mitotic index decreased with increasing doses of L-fucose and L-rhamnose. Prolonged survival of tumour bearing mice was observed after 14 consecutive days of daily administering L-rhamnose. Its optimal dose was estimated to be 3.64 g/kg/day. L-Fucose, however, displayed only a slight effect on the survival of the mice. Our results suggest that L-fucose and especially L-rhamnose have anticancer potential. This study is the first to demonstrate the tumour-inhibitory effect of L-rhamnose.
Histological Marker to Differentiate Organogenic Calli from Non Organogenic Calli of Gloriosa superba L.
Authors: Madhavan Manju , Joseph Joy P. 
Published: August 2010

A histological study of the indirect organogenesis from internodal cultures of Gloriosa superba L. showed that the callus initiated from the subepidermal cells. The organogenic and non organogenic callii are the result of hormonal variation in the medium. In non organogenic callus cells redifferentiated into xylem elements forming clusters of nest like structures. In organogenic callus, cells redifferen-tiated into nodules of meristemoids which further differentiated into shoot apical meristem. Keywords: Histological marker, Gloriosa superba, Organogenic calli D.O.I. 10.3329/ptcb.v20i1.5957 Plant Tissue Cult. & Biotech. 20(1): 1-5, 2010 (June)
Micropropagation of Ophiorrhiza eriantha Wight. through Leaf Explant Cultures
Authors: Rani V. K. Jaimsha , Fijesh P. V. , Padikkala Jose 
Published: August 2010

Leaf explants of Ophiorrhiza eriantha cultured in MS supplemented with the combination of NAA 4 mg/l and BA 0.5 mg/l induced higher callus growth. The maximum number of shoots were produced from callus on the MS supplemented with BA 5 mg/l. The regenerated shoots were transferred into the auxin containing medium for rooting and IBA 3 mg/l supplemented medium produced maximum number of roots per shoot. Camptothecin (anticancer drug) was isolated from O. eriantha wild grown plant and in vitro regenerated plants, and was confirmed by LC-MS-MS. The camptothecin content in wild grown plant, callus and regenerated plants were quantified by HPLC system. Key words: Camptothecin, Leaf explants, Ophiorrhiza eriantha, Micropropagation D.O.I. 10.3329/ptcb.v20i1.5960 Plant Tissue Cult. & Biotech. 20(1): 13-20, 2010 (June)