bluuebunny/crossref_metadata_2025_split huggingface dataset:
SYTO9 and SYBR GREEN1 with a high‐resolution melting analysis for prenatal diagnosis of β0‐thalassemia/hemoglobin‐E
Authors: Pornprasert Sakorn , Sukunthamala Kanyakan 
Published: August 2010

AbstractThe β0‐thalassemia/Hb‐E causes a wide range of severe conditions. A high medical cost is incurred in severe cases. Thus, the prevention of new cases of β0‐thalassemia/Hb‐E is required. The aim of this study is to use the SYTO9 and SYBR GREEN1 high‐resolution melting (HRM) analysis for prenatal diagnosis of β0‐thalassemia/Hb‐E. DNA samples were extracted from amniotic fluid or cord blood of 11 pregnancies whose fetuses were at risk for β‐thalassemia/Hb‐E. PCR products from multiplex amplification refractory mutation system PCR for the detection of β0‐thalassemia mutations at codons 17(A>T), 41/42(−TCTT), and 71/72(+A) and from amplification refractory mutation system PCR for the detection of Hb‐E were characterized by SYTO9 HRM analysis. Moreover, β0‐thalassemia 3.5‐ kb deletion was detected using real‐time PCR with SYBR GREEN1 HRM analysis. Seven of 11 fetuses (64%) were diagnosed as β0‐thalassemia/Hb‐E (4 fetuses with mutation at codon 17, 2 with mutation at codon 41/42, and 1 with 3.5‐ kb deletion). Results from HRM analysis were completely consistent with those from fetal blood samplings analyzed at the time of delivery or pregnancy termination using HPLC. Therefore, the HRM analysis is easy to use. It is simple, flexible, non‐destructive and has superb sensitivity and specificity. This approach might facilitate the laboratory diagnosis and genetic counseling for regions with a high prevalence of β0‐thalassemia/Hb‐E.
Dermatological feasibility of multimodal facial color imaging modality for cross‐evaluation of facial actinic keratosis
Authors: Bae Youngwoo , Son Taeyoon , Stuart Nelson J. , Kim Jae‐Hong , Choi Eung Ho , Jung Byungjo 
Published: August 2010

Background/Purpose: Digital color image analysis is currently considered as a routine procedure in dermatology. In our previous study, a multimodal facial color imaging modality (MFCIM), which provides a conventional, parallel‐ and cross‐polarization, and a fluorescent color image, was introduced for objective evaluation of various facial skin lesions. This study introduces a commercial version of MFCIM, DermaVision‐PRO, for routine clinical use in dermatology and demonstrates its dermatological feasibility for cross‐evaluation of skin lesions.Methods/Results: Sample images of subjects with actinic keratosis or non‐melanoma skin cancers were obtained at four different imaging modes. Various image analysis methods were applied to cross‐evaluate the skin lesion and, finally, to extract valuable diagnostic information. DermaVision‐PRO is potentially a useful tool as an objective macroscopic imaging modality for quick prescreening and cross‐evaluation of facial skin lesions.Conclusion: DermaVision‐PRO may be utilized as a useful tool for the cross‐evaluation of widely distributed facial skin lesions and as an efficient database management of patient information.
Reduction of 4,4′‐Stilbenequinone and 4,4′‐Diphenoquinone upon Reaction with Photogenerated Radicals
Authors: Görner Helmut 
Published: August 2010

AbstractThe properties of 3,3′,5,5′‐tetra‐tert‐butyl‐4,4′‐stilbenequinone (StQ) were studied by photochemical means. Acetone, acetophenone or benzophenone was photolyzed in the presence of both StQ and a donor, such as alcohols or triethylamine. This initiated reaction of a ketyl radical with StQ to form a semiquinone radical and eventually induce a permanent bleaching due to conversion of StQ to 4,4′‐dihydroxystilbene (StQH2). The quantum yield of conversion of StQ to StQH2 increases with the donor concentration. Similar effects were found for the ketone‐sensitized radical‐induced conversions of the analogous diphenoquinone to the reduction product, diphenol.
Intratumoral Localization of Aromatase and Interaction between Stromal and Parenchymal Cells in the Non–Small Cell Lung Carcinoma Microenvironment
Authors: Miki Yasuhiro , Suzuki Takashi , Abe Keiko , Suzuki Satoshi , Niikawa Hiromichi , Iida Shinya , Hata Shuko , Akahira Jun-ichi , Mori Kazushige , Evans Dean B. , Kondo Takashi , Yamada-Okabe Hisafumi , Sasano Hironobu 
Published: August 2010

Abstract
Estrogens produced as a result of intratumoral aromatization has been recently shown to play important roles in proliferation of human non–small cell lung carcinomas (NSCLC), but the details have remained largely unknown. Therefore, in this study, we evaluated the possible roles of intratumoral aromatase in NSCLCs as follows: (a) evaluation of intratumoral localization of aromatase mRNA/protein in six lung adenocarcinoma cases using laser capture microdissection combined with quantitative reverse transcriptase-PCR and immunohistochemistry; (b) examination of the possible effects of isolated stromal cells from lung carcinoma tissues on aromatase mRNA transcript expression in lung carcinoma cell lines (A549 and LK87) through a coculture system; and (c) screening of cytokines derived from stromal LK001S and LK002S cells using cytokine antibody arrays and subsequent evaluation of effects of these cytokines on aromatase expression in A549 and LK87. Both aromatase mRNA and protein were mainly detected in intratumoral carcinoma cells but not in stromal cells. Aromatase expression of A549 and LK87 was upregulated in the presence of LK001S or LK002S cells. Several cytokines such as interleukin-6 (IL-6), oncostatin M, and tumor necrosis factor-α, all known as inducible factors of aromatase gene, were detected in conditioned media of LK001S and LK002S cells. Treatment of both oncostatin M and IL-6 induced aromatase gene expression in A549 an LK87, respectively. These results all indicated that intratumoral microenvironments, especially carcinoma-stromal cell interactions, play a pivotal role in the regulation of intratumoral estrogen synthesis through aromatase expression in human lung adenocarcinomas. Cancer Res; 70(16); 6659–69. ©2010 AACR.
Presence of a Putative Tumor-Initiating Progenitor Cell Population Predicts Poor Prognosis in Smokers with Non–Small Cell Lung Cancer
Authors: Ooi Aik T. , Mah Vei , Nickerson Derek W. , Gilbert Jennifer L. , Ha Vi Luan , Hegab Ahmed E. , Horvath Steve , Alavi Mohammad , Maresh Erin L. , Chia David , Gower Adam C. , Lenburg Marc E. , Spira Avrum , Solis Luisa M. , Wistuba Ignacio I. , Walser Tonya C. , Wallace William D. , Dubinett Steven M. , Goodglick Lee , Gomperts Brigitte N. 
Published: August 2010

AbstractSmoking is the most important known risk factor for the development of lung cancer. Tobacco exposure results in chronic inflammation, tissue injury, and repair. A recent hypothesis argues for a stem/progenitor cell involved in airway epithelial repair that may be a tumor-initiating cell in lung cancer and which may be associated with recurrence and metastasis. We used immunostaining, quantitative real-time PCR, Western blots, and lung cancer tissue microarrays to identify subpopulations of airway epithelial stem/progenitor cells under steady-state conditions, normal repair, aberrant repair with premalignant lesions and lung cancer, and their correlation with injury and prognosis. We identified a population of keratin 14 (K14)–expressing progenitor epithelial cells that was involved in repair after injury. Dysregulated repair resulted in the persistence of K14+ cells in the airway epithelium in potentially premalignant lesions. The presence of K14+ progenitor airway epithelial cells in NSCLC predicted a poor prognosis, and this predictive value was strongest in smokers, in which it also correlated with metastasis. This suggests that reparative K14+ progenitor cells may be tumor-initiating cells in this subgroup of smokers with NSCLC. Cancer Res; 70(16); 6639–48. ©2010 AACR.
Metastasis-Associated Protein 1 Short Form Stimulates Wnt1 Pathway in Mammary Epithelial and Cancer Cells
Authors: Kumar Rakesh , Balasenthil Seetharaman , Pakala Suresh B. , Rayala Suresh K. , Sahin Aysegul A. , Ohshiro Kazufumi 
Published: August 2010

Abstract
Although Wnt1 downstream signaling components as well as cytoplasmic level of metastatic tumor antigen 1 short form (MTA1s) are elevated in human breast cancer, it remains unknown whether a regulatory cross-talk exists between these two pathways. Here, we provide evidence of a remarkable correlation between the levels of MTA1s and stimulation of the Wnt1 signaling components, leading to increased stabilization of β-catenin and stimulation of Wnt1 target genes in the murine mammary epithelial and human breast cancer cells. We found that MTA1s influences Wnt1 pathway through extracellular signal-regulated kinase (ERK) signaling as selective silencing of the endogenous MTA1s or ERK, or its target glycogen synthase kinase 3β resulted in a substantial decrease in β-catenin expression, leading to the inhibition of Wnt1 target genes. Furthermore, downregulation of β-catenin in cells with elevated MTA1s level was accompanied by a corresponding decrease in the expression of Wnt1 target genes, establishing a mechanistic role for the ERK/glycogen synthase kinase 3β/β-catenin pathway in the stimulation of the Wnt1 target genes by MTA1s in mammary epithelial cells. In addition, mammary glands from the virgin MTA1s transgenic mice mimicked the phenotypic changes found in the Wnt1 transgenic mice and exhibited an overall hyperactivation of the Wnt1 signaling pathway, leading to increased stabilization and nuclear accumulation of β-catenin. Mammary glands from the virgin MTA1s-TG mice revealed ductal hyperplasia and ductal carcinoma in situ, and low incidence of palpable tumors. These findings reveal a previously unrecognized role for MTA1s as an important modifier of the Wnt1 signaling in mammary epithelial and cancer cells. Cancer Res; 70(16); 6598–608. ©2010 AACR.
In vivo effects of adjunctive tetracycline treatment on refractory corneal ulcers in dogs
Authors: Chandler Heather L. , Gemensky-Metzler Anne J. , Bras I. Dineli , Robbin-Webb Terah E. , Saville William J. A. , Colitz Carmen M. H. 
Published: August 2010

Abstract
Objective—To evaluate effect of adjunctive treatment with tetracycline analogues on time to complete corneal reepithelialization in dogs with nonhealing (ie, refractory) corneal ulcers.
Design—Randomized controlled clinical trial.
Animals—89 dogs with refractory corneal ulcers.
Procedures—Corneal ulcers were treated via debridement and grid keratotomy. Dogs were assigned to receive 1 of 3 treatment regimens for up to 6 weeks: doxycycline (5 mg/kg [2.27 mg/lb], PO, q 12 h) with topically applied ophthalmic ointment containing neomycin, polymyxin B, and bacitracin (ie, triple antibiotic ointment; q 8 h); cephalexin (22 mg/kg [10 mg/lb], PO, q 12 h) with topically applied oxytetracycline ophthalmic ointment (q 8 h); or a control treatment of cephalexin (22 mg/kg, PO, q 12 h) with topically applied triple antibiotic ointment (q 8 h). Healing was monitored via measurements of the wound with calipers and evaluation of photographs obtained every 2 weeks. Treatment effectiveness was evaluated by wound healing and decreased signs of pain.
Results—The Boxer breed was overrepresented in all groups. At the 2-week time point, wound healing was significantly more common in small-breed dogs, compared with large-breed dogs. Dogs treated with oxytetracycline ophthalmic ointment had a significantly shorter healing time than did dogs receiving the control treatment. Corneal ulcers in dogs that received doxycycline PO healed more rapidly than did ulcers in dogs in the control treatment group; however, this difference was not significant.
Conclusions and Clinical Relevance—Topical tetracycline ophthalmic ointment was a safe, inexpensive, and effective adjunctive treatment for refractory corneal ulcers in dogs.
Effects of infiltration of the incision site with bupivacaine on postoperative pain and incisional healing in dogs undergoing ovariohysterectomy
Authors: Fitzpatrick Courtney L. , Weir Heather L. , Monnet Eric 
Published: August 2010

Abstract
Objective—To determine the effects of infiltration of the incision site with bupivacaine hydrochloride as part of a multimodal analgesia protocol (incisional block) on postoperative analgesia and incisional healing.
Design—Randomized controlled clinical trial.
Animals—92 shelter-owned female dogs undergoing routine ovariohysterectomy.
Procedures—As part of a multimodal analgesic protocol for ovariohysterectomy, dogs received 1 of the following treatments at the incision site: no injection (26 dogs), preincisional infiltration with saline (0.9% NaCl) solution (12 dogs) or bupivacaine (21 dogs), or postincisional infiltration with bupivacaine (33 dogs). Postoperative pain was assessed with the Glasgow pain scale and response to mechanical stimulation with von Frey filaments. Incisions were monitored for signs of inflammation (edema, erythema, and discharge) and complications in wound healing.
Results—There was no difference in pain scores or response to mechanical stimulation over time among treatments. There were no significant differences in incisional edema or discharge among treatments. There was significantly more erythema in dogs that received preincisional infiltration with saline solution at 4 hours after surgery and less erythema in dogs that received postincisional infiltration with bupivacaine at 24 hours after surgery, compared with other treatments. The number of complications for dogs that had preincisional infiltration of bupivacaine was higher than for dogs that had other treatments; complications included excessive inflammation, splenic laceration, and herniation.
Conclusions and Clinical Relevance—No additional analgesic benefit was found in dogs that underwent local bupivacaine infiltration as part of a multimodal analgesic protocol for ovariohysterectomy.
Agreement between directly measured blood pressure and pressures obtained with three veterinary-specific oscillometric units in cats
Authors: Acierno Mark J. , Seaton Diana , Mitchell Mark A. , da Cunha Anderson 
Published: August 2010

Abstract
Objective—To determine whether veterinary-specific oscillometric blood pressure units yield measurements that are in good agreement with directly measured blood pressures in cats.
Design—Evaluation study.
Animals—21 cats undergoing routine spaying or neutering.
Procedures—A 24-gauge catheter was inserted in a dorsal pedal artery, and systolic, diastolic, and mean arterial pressures were directly measured with a validated pressure measurement system. Values were compared with indirect blood pressure measurements obtained with 3 veterinary-specific oscillometric blood pressure units.
Results—There was poor agreement between indirectly and directly measured blood pressures. For unit 1, bias between indirectly and directly measured values was −14.9 mm Hg (95% limits of agreement [LOA], −52.2 to 22.4 mm Hg), 4.4 mm Hg (95% LOA, −26.0 to 34.8 mm Hg), and −1.3 mm Hg (95% LOA, −26.7 to 24.1 mm Hg) for systolic, diastolic, and mean arterial pressures, respectively. For unit 2, bias was −10.3 mm Hg (95% LOA, −52.9 to 32.2 mm Hg), 13.0 mm Hg (95% LOA, −32.1 to 58.0 mm Hg), and 9.1 mm Hg (95% LOA, −32.9 to 51.2 mm Hg) for systolic, diastolic, and mean arterial pressures, respectively. For unit 3, bias was −13.4 mm Hg (95% LOA, −51.8 to 25.1 mm Hg), 8.0 mm Hg (95% LOA, −25.5 to 41.6 mm Hg), and −3.6 mm Hg (95% LOA, −31.6 to 24.5 mm Hg) for systolic, diastolic, and mean arterial pressures, respectively.
Conclusions and Clinical Relevance—Results suggested that none of the 3 veterinary-specific oscillometric blood pressure units could be recommended for indirect measurement of blood pressure in cats.
ChemInform Abstract: Structural and Magnetic Properties of Mn2‐xFexSb (0 < x ≤ 0.7) Solid Solutions.
Authors: RYZHKOVSKII V. M. , DYMONT V. P. , EROFEENKO Z. L. , NEKRASHEVICH E. M. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of a Family of Fine‐Tunable New Chiral Ligands for Catalytic Asymmetric Synthesis. Ligand Optimization Through the Enantioselective Addition of Diethylzinc to Aldehydes.
Authors: VIDAL‐FERRAN A. , MOYANO A. , PERICAS M. A. , RIERA A. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Asymmetric Michael Addition of Nitroalkanes to Prochiral Acceptors Catalyzed by Proline Rubidium Salts.
Authors: YAMAGUCHI M. , IGARASHI Y. , REDDY R. S. , SHIRAISHI T. , HIRAMA M. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Highly Enantiofacial Protonation of Prochiral Lithium Enolates with Chiral β‐Hydroxy Sulfoxides.
Authors: KOSUGI H. , HOSHINO K. , UDA H. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Experimental Test of Setting Three Contiguous Stereogenic Centers in Water. Diastereoselective Coupling of Geometrically Biased Allylic Bromides to α‐Oxy Aldehydes with Indium.
Authors: ISAAC M. B. , PAQUETTE L. A. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Rearrangements of Carbocation Sulfinate Ion Pairs
Authors: HERPERS E. , KIRMSE W. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Useful Oxidation Procedure for the Preparation of 3‐Alkanoyltetronic Acids.
Authors: MITTRA A. , YAMASHITA M. , KAWASAKI I. , MURAI H. , YOSHIOKA T. , OHTA S. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Mild α‐Fluorination of Enantiomerically Pure β‐Ketosulfoxides by F‐TEDA‐BF4.
Authors: ARNONE A. , BRAVO P. , FRIGERIO M. , SALANI G. , VIANI F. , ZANDA M. , ZAPPALA C. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Nonionic Superbase‐Catalyzed Silylation of Alcohols.
Authors: D'SA B. A. , MCLEOD D. , VERKADE J. G. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Rhodium‐Catalyzed Arylation of Aldehydes with Arylstannanes.
Authors: OI S. , MORO M. , INOUE Y. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Stereoselective Synthesis of Conjugated all‐trans‐Tetraenes. Application to the Synthesis of β‐Parinaric Acid Methyl Ester.
Authors: SOLLADIE G. , KALAI C. , ADAMY M. , COLOBERT F. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Ethyl 3‐Iodo‐4,4,4‐trifluoro‐2(Z)‐butenoate: Regio‐ and Stereospecific Preparation and Palladium‐Catalyzed Reaction with Terminal Alkynes.
Authors: QING F.‐L. , ZHANG Y. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Chemistry of ((Perfluoroalkyl)methyl)oxiranes. Regioselectivity of Ring Opening with O‐Nucleophiles and the Preparation of Amphiphilic Monomers.
Authors: CIRKVA V. , AMEDURI B. , BOUTEVIN B. , PALETA O. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Aziridines as Precursors for Chiral Amide‐Containing Surfactants.
Authors: SOMMERDIJK N. A. J. M. , BUYNSTERS P. J. J. A. , AKDEMIR H. , GEURTS D. G. , NOLTE R. J. M. , ZWANENBURG B. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Preparation of N,N′‐Bis(aryl)guanidines from Electron‐Deficient Amines via Masked Carbodiimides.
Authors: BARVIAN M. R. , SHOWALTER H. D. H. , DOHERTY A. M. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: α‐(Phenylthio)cyclopropylation of Carbonyl Compounds: Preparation of α‐Cyclopropyl Ketones.
Authors: POHMAKOTR M. , THISAYUKTA J. 
Published: August 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.