bluuebunny/crossref_metadata_2025_split huggingface dataset:
Regulation of CXCR4 function by S1P1 through heteromerization
Authors: Kim Hyun-Tae , Jeong Jae-Yeon , Huh Won-Ki 
Published: February 2025

Abstract

Background
The trafficking of immune cells between lymphoid organs and circulation depends on gradients of CXCL12 and sphingosine-1-phosphate (S1P), mediated through their cognate receptors C-X-C chemokine receptor type 4 (CXCR4) and S1P receptor type 1 (S1P1). S1P1 facilitates the egress of hematopoietic stem cells and lymphocytes by counteracting CXCR4-mediated retention signals. However, the molecular mechanisms underlying this interplay remain poorly understood. In this study, we uncover CXCR4-S1P1 heteromerization and explore their functional interactions.

Methods
Bimolecular fluorescence complementation (BiFC) assay, proximity ligation assay (PLA), and quantitative bioluminescence resonance energy transfer (BRET) assay were employed to detect CXCR4-S1P1 heteromerization. Functional properties of the heteromers were assessed using cAMP assay, G protein activation, β-arrestin recruitment, ligand binding, calcium mobilization, and transwell migration assays. S1P1-overexpressing Jurkat T cells were generated via lentiviral transduction, while S1P1-deficient KARPAS299 cells and β-arrestin1/2-deficient HEK293A cells were constructed using the CRISPR/Cas9 system.

Results
CXCR4-S1P1 heteromerization was observed in HEK293A cells overexpressing both receptors. The S1P/S1P1 axis interfered with CXCR4-mediated signaling, while CXCR4 did not affect S1P1-mediated signaling, indicating a unidirectional modulation of CXCR4 by S1P1. CXCL12 binding to CXCR4 remained unchanged in the presence of S1P1, and interference of CXCL12-induced Gαi activation by S1P1 was observed in β-arrestin1/2-deficient cells. BRET analysis revealed that S1P1 interfered with CXCR4-Gαi pre-association and CXCR4 oligomerization, both of which are critical for CXCR4 function. Domain-swapping experiments identified transmembrane domain 3 of S1P1 as essential for this modulation. In Jurkat T cells overexpressing S1P1, CXCR4-mediated signaling and cell migration were diminished, whereas these functions were enhanced in S1P1-deficient KARPAS299 cells. Co-activation of S1P1 attenuated CXCL12-induced migration, while pretreatment with S1P or FTY720-phosphate increased CXCR4-mediated migration by downregulating surface S1P1 in KARPAS299 cells. In primary T cells, PLA confirmed CXCR4-S1P1 heteromerization, and S1P interfered with CXCL12-induced migration.

Conclusions
This study identifies CXCR4-S1P1 heteromers and demonstrates a unidirectional modulation of CXCR4 by S1P1. S1P1 affects CXCR4 function by disrupting its G protein pre-association and oligomerization. These findings underscore the regulatory role of the S1P/S1P1 axis in CXCR4 signaling within the heteromeric context and provide novel insights into the intricate mechanisms governing immune cell trafficking.
Diversity and natural infection of phlebotomine sand flies (Diptera, Psychodidae) in an endemic area of American tegumentary leishmaniasis in southeastern Bahia, Brazil
Authors: Cova Bruno Oliveira , de Oliveira Livia Alves , Lima Machado Paulo Roberto , de Carvalho Edgar Marcelino , Monte-Alegre Adriano Figueiredo , Schriefer Albert 
Published: February 2025

Abstract

Background
The Cacao Region spans several municipalities in the state of Bahia. It is one of the major foci of American tegumentary leishmaniasis (ATL) in Brazil. We report the findings of a pilot cross-sectional study describing the phlebotomine fauna found around living sites of newly diagnosed ATL cases in that area.

Methods
The sand fly fauna was studied from May 2018 to June 2019 via an entomological survey, as recommended by the Brazilian Ministry of Health.

Results
Six hundred nineteen phlebotomine sand flies of 20 species were captured: 272 males (44%) and 347 females (56%). Nyssomyia whitmani was the most prevalent (62.2%), followed by Nyssomyia intermedia (9.2%), Evandromyia bahiensis (6.3%), endemic to Bahia, and Trichophoromyia viannamartinsi (4.5%). Ninety-four percent of the female sand flies collected were screened for infection with Leishmania (Viannia) braziliensis by polymerase chain reaction (PCR). Of the 97 sand fly pools analyzed, seven were positive for L. (V.) braziliensis: three of Nyssomyia whitmani, two of Th. viannamartinsi and one each of Psychodopygus hirsutus hirsutus and Trichopygomyia longispina. The overall value of minimum infection rate (MIR) was 2.2%, and its stratification rates for the above species were 1.9, 10, 33 and 50%, respectively. All positive pools consisted of phlebotomine sand flies collected from the peridomiciles and extradomiciles of homes in the municipality of Taperoá in July 2018, resulting in an adjusted MIR of 7.8%, with 50% of the pools positive for L. (V.) braziliensis during that outbreak of ATL.

Conclusions
Our findings suggest that areas experiencing outbreaks of ATL in affected regions present high proportions of infected phlebotomine sand flies involving a variety of species, some not usually considered involved in the L. (V.) braziliensis transmission cycle, such as Th. viannamartinsi.

Graphical Abstract
On the origin of the P-element invasion in Drosophila simulans
Authors: Wierzbicki Filip , Pianezza Riccardo , Selvaraju Divya , Eller Madeleine Maria , Kofler Robert 
Published: February 2025

Abstract
The horizontal transfer (HT) of the P-element is one of the best documented cases of the HT of a transposable element. The P-element invaded natural D. melanogaster populations between 1950 and 1980 following its HT from Drosophila willistoni, a species endemic to South and Central America. Subsequently, it spread in D. simulans populations between 2006 and 2014, following a HT from D. melanogaster. The geographic region where the spread into D. simulans occurred is unclear, as both involved species are cosmopolitan. The P-element differs between these two species by a single base substitution at site 2040, where D. melanogaster carries a ‘G’ and D. simulans carries an ‘A’. It has been hypothesized that this base substitution was a necessary adaptation that enabled the spread of the P-element in D. simulans, potentially explaining the 30-50-year lag between the invasions of D. melanogaster and D. simulans. To test this hypothesis, we monitored the invasion dynamics of P-elements with both alleles in experimental populations of D. melanogaster and D. simulans. Our results indicate that the allele at site 2040 has a minimal impact on the invasion dynamics of the P-element and, therefore, was not necessary for the invasion of D. simulans. However, we found that the host species significantly influenced the invasion dynamics, with higher P-element copy numbers accumulating in D. melanogaster than in D. simulans. Finally, based on SNPs segregating in natural D. melanogaster populations, we suggest that the horizontal transfer of the P-element from D. melanogaster to D. simulans likely occurred around Tasmania.
Fungi stimulate organic phosphorus fraction transformation in subtropical masson pine plantation soils after nine years of thinning and understory removal
Authors: Jian Zunji , Zeng Lixiong , Lei Lei , Frey Beat , Liu Changfu , Shen Yafei , Zhang Jiajia , Xiao Wenfa , Li Mai-He 
Published: February 2025

Abstract

Background
Phosphorus (P) availability in soils regulates forest productivity. However, the drivers of soil P dynamics following forest management remain poorly understood, particularly in P-deficient forests in tropical and subtropical regions.

Method
Soil samples of 0–10 cm were collected from the plots after 9 years of thinning and understory removal (UR) in Pinus massoniana plantations in subtropical China. Soil physicochemical properties, microbial biomass and community composition, and Hedley P fractions were measured to assess the underlying mechanisms for the dynamics of soil P fractions.

Results
Compared to undisturbed plots, total soil inorganic P (Pi; + 24%) within the dominant species thinning (DST) plots showed a significant increase, which was associated with the accrual in resin-Pi (+ 30%), NaHCO3-Pi (+ 21%), and C.CHl-Pi (+ 45%). These Pi fractions were primarily correlated with increased relative abundance of Ascomycota, Rozellomycota, and Proteobacteria. Conversely, post-management (thinning and UR) assessments revealed no significant changes in total P, total organic P (Po), and residual P. The observed decrease in total Po (– 9%) in DST plots was linked to reductions in NaHO-Po (– 7%) and C.CHl-Po (– 24%). Notably, these Po fractions were negatively affected by the relative abundance of Glomeromycota. Furthermore, variations in soil fungal and bacterial community structures accounted for 44.3% and 26.3% of the variances in soil Pi fractions, respectively, similarly explaining 20.4% and 33.3% for soil Po fractions, respectively.

Conclusions
These results indicate that P availability following forest management interventions within subtropical pine plantations is intricately connected to microbial community composition that enhances the transformation from Po into Pi forms.
Targeting novel regulated cell death: disulfidptosis in cancer immunotherapy with immune checkpoint inhibitors
Authors: Du Fei , Wang Guojun , Dai Qian , Huang Jiang , Li Junxin , Liu Congxing , Du Ke , Tian Hua , Deng Qiwei , Xie Longxiang , Zhao Xin , Zhang Qimin , Yang Lan , Li Yaling , Wu Zhigui , Zhang Zhuo 
Published: February 2025

Abstract
The battle against cancer has evolved over centuries, from the early stages of surgical resection to contemporary treatments including chemotherapy, radiation, targeted therapies, and immunotherapies. Despite significant advances in cancer treatment over recent decades, these therapies remain limited by various challenges. Immune checkpoint inhibitors (ICIs), a cornerstone of tumor immunotherapy, have emerged as one of the most promising advancements in cancer treatment. Although ICIs, such as CTLA-4 and PD-1/PD-L1 inhibitors, have demonstrated clinical efficacy, their therapeutic impact remains suboptimal due to patient-specific variability and tumor immune resistance. Cell death is a fundamental process for maintaining tissue homeostasis and function. Recent research highlights that the combination of induced regulatory cell death (RCD) and ICIs can substantially enhance anti-tumor responses across multiple cancer types. In cells exhibiting high levels of recombinant solute carrier family 7 member 11 (SLC7A11) protein, glucose deprivation triggers a programmed cell death (PCD) pathway characterized by disulfide bond formation and REDOX (reduction-oxidation) reactions, termed “disulfidptosis.” Studies suggest that disulfidptosis plays a critical role in the therapeutic efficacy of SLC7A11high cancers. Therefore, to investigate the potential synergy between disulfidptosis and ICIs, this study will explore the mechanisms of both processes in tumor progression, with the goal of enhancing the anti-tumor immune response of ICIs by targeting the intracellular disulfidptosis pathway.
A lightweight resonance tracking system for piezoresistive microcantilever sensors
Authors: Setiono Andi , Nelfyenny  , Suryadi  , Qomaruddin  , Nyang’au Wilson Ombati , Peiner Erwin 
Published: February 2025

Abstract
A lightweight resonance tracking system designed for precise monitoring of resonant frequency shifts in microcantilever sensors is introduced. The system integrates a Phase-Locked Loop (PLL)-based technique with a Python-based interface for real-time control and visualization. A tipless microcantilever sensor was tested under relative humidity (RH) conditions ranging from 63% to 90% to experimentally validate the system. The system demonstrated a sensitivity of 1.082 Hz/% RH and a Limit of Detection (LOD) of 1.89% RH. The silicon dioxide (

$$SiO_2$$

S
i

O
2

) surface of the microcantilever is hygroscopic, allowing water vapor adsorption and causing frequency shifts. This effect is more pronounced at high humidity levels (>80% RH) due to multilayer adsorption. These results confirm the reliability and precision of the system in detecting environmental changes. The findings highlight the potential of the developed system for applications in environmental monitoring, healthcare diagnostics, and industrial chemical sensing.
From portfolio optimization to quantum blockchain and security: a systematic review of quantum computing in finance
Authors: Naik Abha Satyavan , Yeniaras Esra , Hellstern Gerhard , Prasad Grishma , Vishwakarma Sanjay Kumar Lalta Prasad 
Published: February 2025

Abstract
The rapid advancement of quantum computing has sparked a considerable increase in research attention to quantum technologies. These advances span fundamental theoretical inquiries into quantum information and the exploration of diverse applications arising from this evolving quantum computing paradigm. The scope of the related research is notably diverse. This paper consolidates and presents quantum computing research related to the financial sector. The finance applications considered in this study include portfolio optimization, fraud detection, and Monte Carlo methods for derivative pricing and risk calculation. In addition, we provide a comprehensive analysis of quantum computing’s applications and effects on blockchain technologies, particularly in relation to cryptocurrencies, which are central to financial technology research. As discussed in this study, quantum computing applications in finance are based on fundamental quantum physics principles and key quantum algorithms. This review aims to bridge the research gap between quantum computing and finance. We adopt a two-fold methodology, involving an analysis of quantum algorithms, followed by a discussion of their applications in specific financial contexts. Our study is based on an extensive review of online academic databases, search tools, online journal repositories, and whitepapers from 1952 to 2023, including CiteSeerX, DBLP, ResearchGate, Semantic Scholar, and scientific conference publications. We present state-of-the-art findings at the intersection of finance and quantum technology and highlight open research questions that will be valuable for industry practitioners and academicians as they shape future research agendas.
Comparative efficacy and safety of drug-coated balloons versus drug-eluting stents in small vessel coronary artery disease: an updated systematic review and meta-analysis of randomized controlled trials
Authors: Augustine Monisha , Arain Mustafa , Mustafa Muhammad Saqlain , Moradi Iman , Fredericks Matthew , Rahman Aaliya , Ashraf Muhammad Afnan , Sualeh Glawish , khan Rubab , Saif Aqsa , Arain Haifa , Rajpurohit Dilip Baldevsingh , Maslamani Abdalkareem Nael Jameel , Shojai Rahnama Behrooz , Iqbal Javed 
Published: February 2025

Abstract

Background
Small vessel coronary artery disease presents challenges in percutaneous coronary intervention due to higher restenosis rates with traditional treatments. Drug-coated balloons (DCBs) offer a potential alternative, but their efficacy compared to drug-eluting stents (DES) remains debated. This meta-analysis aims to provide updated insights into the comparative outcomes of DCBs versus DES in small coronary artery disease.

Main text
Following PRISMA guidelines, a systematic review identified seven randomized controlled trials (RCTs) comparing DCBs with DES for small vessel CAD. Data were extracted and pooled for analysis, assessing outcomes including target lesion revascularization (TLR), target vessel revascularization (TVR), mortality, myocardial infarction (MI), stent/vessel thrombosis, and major adverse cardiovascular events (MACE). Statistical analysis was performed using RevMan version 5.4, employing random-effects models and forest plots with odds ratios (OR) and 95% confidence intervals (CI). Among 1,808 patients across seven RCTs, no significant difference was found in TVR between DCB and DES over 3 years (OR = 0.95, 95% CI [0.58, 1.54], p = 0.82). While initial analyses favoured higher TLR incidence in DES, the trend shifted towards DCB over time, with a non-significant association favouring DCB at 3 years (OR = 0.51, 95% CI [0.26, 1.00], p = 0.05). DCB use was associated with significantly higher rates of MACE and MI at the 3-year mark (MACE: OR = 0.55, 95% CI [0.38, 0.79], p = 0.001; MI: OR = 0.35, 95% CI [0.17, 0.7], p = 0.003), while mortality rates converged between the two interventions over time. Vessel thrombosis rates were similar between DCB and DES.

Conclusions
While DCBs may offer comparable efficacy to DES in terms of TVR and TLR over shorter durations, there is a concerning trend towards higher rates of MACE and MI associated with DCB use at the 3-year mark. Further research with larger sample sizes, longer follow-up durations, and consistent inclusion criteria is needed to elucidate the optimal treatment strategy for small vessel CAD. Until then, DES may be considered a safer option for managing small vessel CAD.
The Drôle de Guerre on the Maginot Line
Authors: Passmore Kevin 
Published: February 2025

Abstract
In September 1939 half the population of Alsace-Lorraine was evacuated or mobilized outside the province and replaced by one million troops from the “interior.” The 1939–40 campaign therefore provides an ideal way to explore Alsace-Lorraine's position in France. While some historians argue that Alsace-Lorrainers’ experience of the Phoney War caused a “crisis of national sentiment,” revisionist military historians present convincing evidence that relations between “interior French” and Alsace-Lorrainers were good. This article uses dialogical methods to reconcile these interpretations and argues that national loyalty was never unconditional or unreflecting. Interior French and Alsace-Lorrainers’ stereotypes concerning each other were contradictory and mediated by mixed views of Germany. Practical interactions between interior French and Alsace-Lorrainers were entangled with gender, class, and religious divides and depended on what was at stake in different contexts. The article shows how relations varied in the pillaged evacuated zone, between soldiers and civilians in nonevacuated villages, and in military units. Even in the extreme situation of combat, protagonists continued to calculate. If anyone experienced a crisis of national sentiment, it was the interior French.
Concurrent invasive ductal carcinoma and ductal carcinoma in situ arising inside and outside a breast hamartoma: A case report
Authors: Wei Lai , Tian Zhe , Wang Zhi-Yong , Liu Wei-Jia , Li Hong-Bo , Zhang Ying 
Published: February 2025

BACKGROUND
Breast hamartomas are rare benign breast tumors, with an incidence rate of 0.8%-4.8%. Further, the coexistence of hamartomas and carcinoma is also uncommon. Our case report presents a unique instance where invasive ductal carcinoma (IDC) and ductal carcinoma in situ were found both inside and outside a breast hamartoma. This is the second case reported in the literature.
CASE SUMMARY
A 51-year-old woman presented with a 6.0 cm breast tumor on mammography and ultrasound, with suspicious areas indicative of malignant transformation. Biopsy of the suspicious area confirmed IDC with intraductal carcinoma. Breast magnetic resonance imaging showed typical hamartoma changes with irregular areas of abnormal enhancement both inside and outside. A breast-conserving surgery was performed, and postoperative pathology confirmed mammary hamartoma, concurrent with IDC and intraductal carcinoma occurring both inside and outside the hamartoma. Subsequently, appropriate adjuvant therapy was initiated. Currently, the patient is in good condition. Breast cancer may be located both inside and outside the ipsilateral mammary hamartoma, which is difficult to detect preoperatively, especially when there is a focus of intraductal carcinoma, requiring accurate assessment of the tumor extent by modern imaging techniques. Early detection of the coexistence of cancer is clinically important as it can alter patient management.
CONCLUSION
This case emphasizes the importance of modern imaging techniques in accurately evaluating mammary hamartomas associated with malignancies prior to surgery.
Refractory Crohn's disease complicated with Guillain-Barré syndrome: A case report
Authors: Liu A-Niu , Yang Jia-Yi , Chen Xing-Yu , Wu Shan-Shan , Ji Zhi Se-Niu , Zheng Shu-Mei 
Published: February 2025

BACKGROUND
Inflammatory bowel disease (IBD) comprises a group of chronic inflammatory gastrointestinal disorders, including Crohn's disease (CD) and ulcerative colitis, with uncertain etiologies. The natural course of IBD can be accompanied by extraintestinal manifestations involving the skin, mucous membranes, musculoskeletal structures, eyes, cardiovascular system and nervous system. Guillain-Barré syndrome (GBS) is a type of peripheral neuropathy. However, the etiology and pathogenesis of IBD combined with GBS are unclear, and only a few clinical cases have been reported. Here, we report a case of refractory CD complicated by GBS and review the previous literature to improve the understanding of these diseases.
CASE SUMMARY
A 34-year-old man had a 9-year history of refractory CD. He became unresponsive to multiple drugs and experienced recurrent intestinal fistulas. After several abdominal surgeries and treatment with ustekinumab, he achieved clinical remission. Unfortunately, he developed GBS during maintenance treatment with ustekinumab. According to previous reports, in some patients with IBD combined with GBS, GBS may be a comorbidity, an extraintestinal manifestation of IBD, or an adverse reaction to IBD therapeutic drugs. After a comprehensive evaluation, we suspected that GBS might have been a comorbidity in this patient. To avoid fatal disease relapse after medication discontinuation, we concluded that ustekinumab should not be withdrawn. On the basis of a joint decision between doctors and the patient, we decided to continue maintenance treatment with ustekinumab along with intravenous immunoglobulin, dexamethasone and traditional Chinese medicine acupuncture, which resulted in a steady improvement in his GBS symptoms and sustained remission of CD.
CONCLUSION
When IBD is complicated by a neurological disease, it is first necessary to analyze the patient's condition and then choose the corresponding treatment strategy. If the neurological disease is a specific comorbidity, treatment of both IBD and the comorbid disease should be considered. For IBD patients with extraintestinal manifestations involving the nervous system, neurological manifestations tend to resolve when the active IBD is controlled. When an adverse drug reaction is suspected, the medication should be discontinued, and symptomatic treatment should be administered.
«Diciendo sí y no a la vida como Cristo nos enseña»: María Zambrano, «Isla de Puerto Rico», Gabriela Mistral
Authors: Fernández Navas David 
Published: February 2025

Estudiamos el discurso de María Zambrano en Isla de Puerto Rico y prestamos especial atención a una enigmática propuesta: "tomar en vilo el peso de la propia vida 'diciendo sí y no como Cristo nos enseña'”. Ello nos llevará al sufismo de Ibn ‘Arabī y, finalmente, a una carta que Zambrano escribe en los años cincuenta a Gabriela Mistral.