bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fabry's Disease: A Prospective Multicenter Cohort Study in Young Adults with Cryptogenic Stroke
Authors: Saposnik Gustavo , Lanthier Sylvain , Mamdani Muhammad , Thorpe Kevin E. , Melo Magda , Pope Karen , Selchen Daniel , Moore David F. ,   on behalf of the Canadian Stroke Consortium and the Stroke Outcome Research Canada (SORCan) Working Group
Published: January 2012

Background Stroke in young adults is etiologically diverse and may represent a diagnostic challenge remaining cryptogenic in one-fourth of cases. Limited information is available on the prevalence of Fabry's disease, a treatable multisystem inherited lysosomal storage disorder, and disability in young patients with cryptogenic stroke. Design and methods The Canadian Fabry Stroke Screening Initiative (CFSSI) is a prospective multicenter cohort study of young adults (age 18–55) presenting with an ischemic stroke, transient ischemic attack, or intracerebral haemorrhage of unknown etiology to stroke centres across Canada. Diagnosis of Fabry's disease is made by direct DNA analysis of blood samples for α-galactosidase gene mutations or polymorphisms. Demographics, clinical information, and investigations including brain Magnetic Resonance Imaging (MRI) are collected. Functional neurological assessment includes neurological examination, the National Institutes of Health (NIH) stroke scale, modified Rankin scale, and the Barthel index. A follow-up interview is conducted by telephone or in person approximately six-months after the index stroke/transient ischemic attack/intracerebral haemorrhage to determine patient outcomes, quality of life, and patient use of medications. Main outcome Prevalence of positive DNA mutation or single nucleotide polymorphism screens for Fabry's disease as a proportion of total cryptogenic stroke. Secondary outcomes include incident risk of new or recurrent vascular event at six-months, discharge disposition, disability at six-months as measured by the modified Rankin scale, mean time from symptoms onset to the definite etiological diagnosis, and length of hospital stay. Conclusion This study constitutes the first initiative to determine the prevalence of a positive screen for Fabry's disease in young adults with stroke in Canada. Moreover, the Canadian Fabry Stroke Screening Initiative will provide information on recurrent vascular events, disability at six-months (modified Rankin scale), and disposition in this understudied population.
Molecular analysis of the β‐globin gene cluster haplotypes in a Sudanese population with sickle cell anaemia
Authors: ELDERDERY A. Y. , MILLS J. , MOHAMED B. A. , COOPER A. J. , MOHAMMED A. O. , ELTIEB N. , OLD J. 
Published: January 2012

SummaryIntroduction:  Sudan has a multiethnic population with a high frequency of Hb S, but little is known about the βS haplotypes in this population.Methods:  Blood samples from Sudanese Hb SS individuals were taken at two locations. Family history, age, ethnicity and clinical symptoms were recorded for each subject. Hb S was investigated using cellulose acetate electrophoresis (CAE) and cation exchange–high performance liquid chromatography. Dried blood samples from 93 individuals were used for βS haplotype identification based on restriction fragment length polymorphism analysis for seven restriction sites.Results:  Haplotypes could be assigned unequivocally to 143 chromosomes. Four of the five typical βS‐globin haplotypes were identified. The most frequent was the Cameroon (35.0%), followed by the Benin (29.4%), the Senegal (18.2%) and the Bantu (2.8%). The Indian‐Arab haplotype was not observed. Three atypical haplotypes were identified in 17 patients, occurring at a combined frequency of 14.6%. One of these, found at the high frequency of 11.8%, possibly represented a new Sudan haplotype.Conclusion:  βS Haplotyes were demonstrated successfully from dried blood samples. A new haplotype is apparent in Sudan, in addition to the four African haplotypes.
SILENCE LENDS INTEGRITY TO SPEECH: TRANSCENDING THE OPPOSITES OF SPEECH AND SILENCE IN THE ANALYTIC DIALOGUE
Authors: Bravesmith Anna 
Published: January 2012

abstractIn this paper the interplay between silence and the spoken words used by analyst and patient will be explored within the context of clinical practice. Both analyst and patient, it is argued, are engaged in a personal struggle to try to discover an integrative connection between silence, often experienced as nothingness, and speech, often experienced as suffocating or mendacious. The uses of silence in aiding speech to attain integrity will be described with reference to two clinical vignettes. Selections from psychoanalytic theory and practice will be discussed, throwing some light on silence and analytic spoken dialogue, and it will be argued that a Jungian perspective contributes a further, unique, insight through the concept of transcendent function. This is a way of seeing silence and speech as opposites, out of which new levels of meaning arise as a result of union and unconscious cooperation in the relationship between them. This union is known as coniunctio.
Behavioural rehabilitation of chronic low back pain: Comparison of an operant treatment, an operant‐cognitive treatment and an operant‐respondent treatment
Authors: Vlaeyen Johan W. S. , Haazen Ingrid W. C. J. , Schuerman Joop A. , Kole‐Snijders Ank M. J. , van Eek Hugo 
Published: January 2012

Seventy‐one chronic low back pain patients were assigned to one of three behavioural rehabilitation treatments or a waiting‐list condition. The first intervention consisted of an operant treatment, aimed at increasing health behaviours and activity levels and at reducing pain and illness behaviours. In the second intervention, a cognitive treatment, aimed at the reinterpretation of catastrophizing pain cognitions and at enhancing self‐control, was combined with an operant treatment. The third intervention consisted of the combination of the operant approach and a respondent treatment. During the respondent treatment, patients were taught to decrease muscle tension levels, using the 'applied relaxation' technique supported by EMG‐biofeedback and graded exposure to tension‐eliciting situations.A repeated measurements design included observer rating of pain behaviours, observer ratings of mood, self‐reported depression, residual health behaviours, pain cognitions and experienced pain intensity. Follow‐up assessment occurred at six months and one year after termination of treatment. Results suggest that, for the sample as a whole, improvements are found on measures of pain behaviours, health behaviours, pain cognitions and affective distress and that these improvements are maintained at six months and one year follow‐up. During the treatment the three treatment groups improved significantly more than the waiting‐list control group on most of the measures. Further, the results of this study provide evidence that the operant‐cognitive and operant‐respondent conditions are more efficacious in decreasing pain behaviours and in increasing health behaviours and efficacy expectations than operant treatment alone. This differential effect among the conditions is maintained at follow‐up. Patients who received the OC and OR treatments catastrophize less than OP patients, and OC patients showed better scores on outcome‐efficacy than OR patients. In general, the results suggest that behavioural rehabilitation programmes for chronic low back pain are effective and that the effects of an operant treatment are magnified when self‐control techniques are added.
The capillary index score: rethinking the acute ischemic stroke treatment algorithm. Results from the Borgess Medical Center Acute Ischemic Stroke Registry
Authors: Al-Ali Firas , Jefferson Anne , Barrow Tom , Cree Travis , Louis Susan , Luke Kim , Major Kevin , Nemeth Daniel , Smoker Sandy , Walker Sarah 
Published: January 2012

BackgroundDespite increased recanalization rates in the treatment of acute ischemic stroke, the percentage of patients with a good clinical outcome of all those treated has not risen above 50%. This 50% barrier may be broken by improving the criteria for treatment selection. This study investigated the addition of the capillary index score (CIS), a new index for assessing remaining viable tissue in the ischemic area, to the existing criteria.MethodsThe Borgess Medical Center Ischemic Stroke Registry is a non-randomized single-center single-operator registry of consecutive subjects admitted for intra-arterial treatment of acute ischemic stroke. The CIS was calculated from a pre-intervention catheter cerebral angiogram in subjects with internal carotid artery (ICA) or middle cerebral artery (MCA) (M1) occlusion. Thrombolysis In Myocardial Infarction (TIMI) 2 or 3 was considered successful recanalization. A modified Rankin Scale (mRS) of 0–2 at 3 months was considered a good outcome.ResultsICA or MCA (M1) occlusion was found in 46 of 58 consecutive patients treated by the same operator. Recanalization was successful in 72% of patients and 27% had a good outcome. CIS was available for 26 patients; 42% were favorable (2 or 3) and 58% were poor (0 or 1). A good outcome was found only in the favorable CIS group (p=0.0148). Successful recanalization (p=0.0029) and time from ictus to revascularization (p=0.0039) predicted a good outcome. Of patients with favorable CIS and TIMI 3, 83% had a good outcome.ConclusionsFavorable CIS and recanalization were strong predictors of a good outcome. By using this new index as an adjunct to other criteria, the CIS may improve patient selection and help break the 50% barrier.
Telemedicine versus face-to-face evaluation in the delivery of thrombolysis for acute ischaemic stroke: a single centre experience
Authors: Chowdhury Muhibbur , Birns Jonathan , Rudd Anthony , Bhalla Ajay 
Published: January 2012

Abstract

Background
Telemedicine is increasingly used in the UK to deliver thrombolysis. It is primarily used to enable assessment of people presenting with an acute stroke by a remote specialist in stroke care, and to determine eligibility for thrombolysis with alteplase (recombinant tissue plasminogen activator). This study aims to evaluate the process of acute stroke care, safety and outcome profiles when comparing face-to-face evaluation and telemedicine in the delivery of thrombolysis.

Methods
This was a retrospective single centre cohort study, evaluating patients thrombolysed from July 2007 to December 2009 inclusive. All patients were given treatment within a 3-hour window from onset of symptoms. Of the 97 patients thrombolysed, 45 (46%) were evaluated by telemedicine. Process times of the steps taken to deliver thrombolysis for the two groups were compared. The authors include the rates of symptomatic intracranial haemorrhage (SICH). Outcome data include 3-month mortality and functional status.

Results
Process times were significantly better in face-to-face: Admission to CT (p=0.001), CT to treatment (p≤0.001) and admission to treatment (p≤0.001). SICH occurred in four patients (7.7%) in the face-to-face group compared with the two patients (4.4%) in the telemedicine group (p=0.7). Favourable outcome: a modified Rankin score of 0–2 was observed in 19 patients (36.5%) in the face-to-face group compared with 19 patients (42%) in the telemedicine group (p=0.9).

Conclusions
This analysis shows that the use of telemedicine compared with face-to-face evaluation is feasible in the delivery of thrombolysis during out of hours. There are several areas of our emergency process of hyper-acute stroke care that need improving when using telemedicine.
Expression of Interleukin-4 Receptor Alpha in Human Pleural Mesothelioma Is Associated with Poor Survival and Promotion of Tumor Inflammation
Authors: Burt Bryan M. , Bader Andrew , Winter Daniel , Rodig Scott J. , Bueno Raphael , Sugarbaker David J. 
Published: January 2012

AbstractPurpose: The origin and pathogenesis of malignant pleural mesothelioma (MPM) are closely aligned with inflammation. MPM tumors express interleukin-4 receptor α (IL-4Rα), the principal subunit of the IL-4 receptor. We set out to determine the biologic function and clinical relevance of IL-4Rα in human MPM.Experimental Design: Expression of IL-4Rα by human MPM tumors was determined by quantitative real-time PCR (n = 37) and immunohistochemistry (n = 52). Intracellular cytokine analysis of T-cell–derived IL-4 was carried out on matched tumor and blood samples from eight patients with MPM. Four human MPM cell lines were used to determine the direct effects of IL-4 on MPM tumor cells.Results: High tumor mRNA expression of IL-4Rα was an independent predictor of poor survival in patients with epithelial MPM [HR, 3.13, 95% confidence interval (CI), 1.68–7.15; P = &lt;0.0001]. Ninety-seven percent of epithelial MPM tumors and 95% of nonepithelial MPM tumors expressed IL-4Rα protein by immunohistochemistry, and strong IL-4Rα staining correlated with worse survival in patients with epithelial histology (P = 0.04). A greater percentage of tumor-infiltrating T cells produced IL-4 compared with matched blood T cells (21% ± 7% vs. 4% ± 2%, P = 0.0002). In response to IL-4, human MPM cells showed increased STAT-6 phosphorylation and increased production of IL-6, IL-8, and VEGF, without effect on proliferation or apoptosis.Conclusions: Tumor expression of IL-4Rα is inversely correlated with survival in patients undergoing surgical resection for epithelial MPM. Tumor-infiltrating T cells in MPMs are polarized to produce IL-4 and may provide endogenous activation signals to MPM tumor cells in situ. The IL-4/IL-4 receptor axis is a potential therapeutic target in human MPM. Clin Cancer Res; 18(6); 1568–77. ©2012 AACR.
Expression of stem cell and epithelial-mesenchymal transition markers in primary breast cancer patients with circulating tumor cells
Authors: Kasimir-Bauer Sabine , Hoffmann Oliver , Wallwiener Diethelm , Kimmig Rainer , Fehm Tanja 
Published: January 2012

Abstract
Introduction
The presence of circulating tumor cells (CTC) in breast cancer might be associated with stem cell-like tumor cells which have been suggested to be the active source of metastatic spread in primary tumors. Furthermore, to be able to disseminate and metastasize, CTC must be able to perform epithelial-mesenchymal transition (EMT). We studied the expression of three EMT markers and the stem cell marker ALDH1 in CTC from 502 primary breast cancer patients. Data were correlated with the presence of disseminated tumor cells (DTC) in the bone marrow (BM) and with clinicopathological data of the patients.

Methods
A total of 2 × 5 ml of blood was analyzed for CTC with the AdnaTest BreastCancer (AdnaGen AG) for the detection of EpCAM, MUC-1, HER2 and beta-Actin transcripts. The recovered c-DNA was additionally multiplex tested for three EMT markers [TWIST1, Akt2, phosphoinositide kinase-3 (PI3Kα)] and separately for the tumor stem cell marker ALDH1. The identification of EMT markers was considered positive if at least one marker was detected in the sample. Two BM aspirates from all patients were analyzed for DTC by immunocytochemistry using the pan-cytokeratin antibody A45-B/B3.

Results
Ninety-seven percent of 30 healthy donor samples investigated were negative for EMT and 95% for ALDH1 transcripts, respectively. CTC were detected in 97/502 (19%) patients. At least one of the EMT markers was expressed in 29% and ALDH1 was present in 14% of the samples, respectively. Interestingly, 5% of the ALDH1-positive and 18% of the EMT-positive patients were CTC-negative based on the cut-off level determined for CTC-positivity applying the AdnaTest BreastCancer. DTC in the BM were detected in 107/502 (21%) patients and no correlation was found between BM status and CTC positivity (P = 0.41). The presence of CTC, EMT and ALDH1 expression was not correlated to any of the prognostic clinical markers.

Conclusions
Our data indicate that (1) a subset of primary breast cancer patients shows EMT and stem cell characteristics and (2) the currently used detection methods for CTC are not efficient to identify a subtype of CTC which underwent EMT. (3) The clinical relevance on prognosis and therapy response has to be further evaluated in a prospective trial.
Conductive Electrospun and Micro-StereoLithographically Produced Porous Scaffolds as Potential Neural Interface Materials
Authors: Dirk Shawn M. , Cicotte Kirsten N. , Hedberg-Dirk Elizabeth L. , Buerger Stephen , Lin Patrick P. , Reece Gregory 
Published: January 2012

ABSTRACTOur overall intent is to develop improved electrically active prosthetic devices to allow interactions between regenerated nerve fibers (axons) and external electronics. To allow for infiltration of axons, these devices must be highly porous. Additionally, they must exhibit selective and structured conductivity to allow the connection of electrode sites with external circuitry with tunable electrical properties that enable the transmission of neural signals through physical connections to external circuitry (e.g. through attached wires.) The chosen material must be biocompatible with minimal irresolvable inflammatory response to allow intimate contact with regenerated nerve tissue and mechanically compatible with the surrounding nervous tissue.We have utilized electrospinning and projection lithography as tools to create conductive, porous networks of non-woven biocompatible fibers in order to meet the materials requirements for the neural interface. The biocompatible fibers were based on the known biocompatible material poly(dimethyl siloxane) (PDMS) as well as a newer biomaterial material developed in our laboratories, poly(butylene fumarate) (PBF). Both of the polymers cannot be electrospun using conventional electrospinning techniques due to their low glass transition temperatures, so in situ crosslinking methodologies were developed to facilitate micro- and nano-fiber formation during electrospinning. The conductivity of the electrospun fiber mats was controlled by varying the loading with multi-walled carbon nanotubes (MWNTs).
Testing the Limits of Small Scale Plasticity with Thin Wires in Torsion
Authors: Bushby Andrew J. , Feuvrier Julien , Dong Dong V. , Dunstan David J. 
Published: January 2012

ABSTRACTTorsion experiments on 50μm diameter Cu wires are reported, using the load/unload method and a gauge length of 1m to obtain torsional strain sensitivity better than unity microstrain. The experiments are able to resolve reversible and irreversible deformation at such very low strains. Plastic deformation, dislocation creep and the Bauschinger effect are easily observed at room temperature and at about 300°C, both in the low-strain regime where it is unlikely that dislocation sources are activated, and at higher plastic strain.
Theory of Fluid Lubrication of Hydrogels and Articular Cartilage during Compression Under an Applied Load
Authors: Sokoloff J. B. 
Published: January 2012

ABSTRACTA fluid lubrication model for articular cartilage was put forward by Mc Cutchen, in which a high percentage of the load is supported by fluid pressurization in the interface region separating the two cartilage coated surfaces as the cartilage is compressed under load. This reduces the friction by reducing the percentage of the load which is carried by solid material in the cartilage. For two bones which are in contact in a healthy joint, which are each coated by a layer of cartilage whose thickness is much smaller than its lateral dimensions, it will be argued that since the bone is impervious to fluid flow in healthy joints, almost all of the fluid that is expressed from the cartilage under load flows through the interface region, where it supports part of the load. This is in contrast to previous theoretical and in vitro experimental studies of this problem, in which most of the fluid does not flow into the interface. It is shown that for mean asperity height small compared to a length scale (which depends on the cartilage or hydrogel permeability, the fluid viscosity and the dimensions of the cartilage or hydrogel) a large percentage of the load is supported by fluid pressurization.
Domination, forcing, array nonrecursiveness and relative recursive enumerability
Authors: Cai Mingzhong , Shore Richard A. 
Published: January 2012

AbstractWe present some abstract theorems showing how domination properties equivalent to being or array nonrecursive can be used to construct sets generic for different notions of forcing. These theorems are then applied to give simple proofs of some known results. We also give a direct uniform proof of a recent result of Ambos-Spies, Ding, Wang, and Yu [2009] that every degree above any in is recursively enumerable in a 1-generic degree strictly below it. Our major new result is that every array nonrecursive degree is r.e. in some degree strictly below it. Our analysis of array nonrecursiveness and construction of generic sequences below ANR degrees also reveal a new level of uniformity in these types of results.
A hierarchy of tree-automatic structures
Authors: Finkel Olivier , Todorčević Stevo 
Published: January 2012

AbstractWe considerωn-automatic structures which are relational structures whose domain and relations are accepted by automata reading ordinal words of lengthωnfor some integern≥ 1. We show that all these structures areω-tree-automatic structures presentable by Muller or Rabin tree automata. We prove that the isomorphism relation forω2-automatic (resp.ωn-automatic forn> 2) boolean algebras (respectively, partial orders, rings, commutative rings, non commutative rings, non commutative groups) is not determined by the axiomatic system ZFC. We infer from the proof of the above result that the isomorphism problem forωn-automatic boolean algebras,n≥ 2, (respectively, rings, commutative rings, non commutative rings, non commutative groups) is neither a-set nor a-set. We obtain that there exist infinitely manyωn-automatic, hence alsoω-tree-automatic, atomless boolean algebras, which are pairwise isomorphic under the continuum hypothesis CH and pairwise non isomorphic under an alternate axiom AT, strengthening a result of [14].
Comments by the Auditing Standards Committee of the Auditing Section of the American Accounting Association on the PCAOB Rulemaking Docket Matter No. 34: PCAOB Release No. 2011-003, Concept Release on Possible Revisions to PCAOB Standards Related to Reports on Audited Financial Statements.
Authors: Brazel Joseph F , Caster Paul , Davis Shawn , Glover Steven M , Janvrin Diane J , Kozloski Thomas M , Pevzner Mikhail 
Published: January 2012

SUMMARY
Recently, the Public Company Accounting Oversight Board (PCAOB or Board) issued a concept release to solicit public comment on the potential direction of a proposed standard-setting project on the content and form of reports on audited financial statements. The objective of the concept release was to discuss several alternatives for changing the auditor's reporting model that could increase its transparency and relevance to financial statement users, while not compromising audit quality. To that end, the alternatives included (1) a supplement to the auditor's report, in which the auditor would be required to provide additional information about the audit and the company's financial statements (an “Auditor's Discussion and Analysis”), (2) required and expanded use of emphasis paragraphs in the auditor's report, (3) auditor reporting on information outside the financial statements, and (4) clarification of certain language in the auditor's report. The PCAOB provided for a 102-day exposure period (from June 21 to September 30, 2011) for interested parties to examine and provide comments on the conceptual approaches to rulemaking that might complement the application of Section 105(c)(6). The Auditing Standards Committee of the Auditing Section of the American Accounting Association provided the comments in the letter below to the PCAOB on the PCAOB Release No. 2011-003, Concept Release on Possible Revisions to PCAOB Standards Related to Reports on Audited Financial Statements.
Data Availability: Information about and access to the release is available at: http://pcaobus.org/Rules/Rulemaking/Docket034/Concept_Release.pdf