bluuebunny/crossref_metadata_2025_split huggingface dataset:
Profile of the first cases hospitalized due to Influenza A (H1N1) in Panama City, Panama. May-June 2009.
Authors: Tulloch Felicia , Correa Ricardo , Guerrero Gladys , Samaniego Rigoberto , Garcia Mariana , Pascale Juan M , Martinez Alexander , Mendoza Yaxelis , Victoria Gerardo , Ng de Lee Marisol , Marchena Loyd , De Mosca Itza Barahona , Armien Blas 
Published: January 2010

Introduction: In April 2009, a novel influenza A (H1N1) virus was identified in patients from Mexico and the United States. From 8 May through 25 June 2009, in the Republic of Panama, 467 cases infected with the same virus were identified, 13 of which were hospitalized at the Santo Tomas Hospital in Panama City. Up to the date of this report, no deaths have been reported in Panama. This study presents the first thirteen cases of Influenza A (H1N1) 2009 that were hospitalized in Panama City. Methodology: The Santo Tomas Hospital (HST), a third-level institution of the Ministry of Health (MINSA) for adult health care (patients above the age of 14), was designated as the reference center for treating these cases. For this purpose, the norms and criteria established by the system were followed and every patient (case) presenting flu-like symptoms was included (fever equal or greater than 38ºC (100.4ºF), cough, sore throat, rhinorrhea, lethargy in children under the age of one, and respiratory distress). Results: Seventy-six patients were hospitalized as suspected cases for infection with the influenza A H1N1 2009 virus, of which 13 (17.1%) were confirmed as positive. The clinical picture was characterized by fever (100%), cough (92.3%), rhinorrhea (69.2%), malaise (53.8%), headache (53.8%), and only one case presented gastrointestinal symptoms (diarrhoea). The male:female ratio was 1:2.2. Conclusion: The knowledge and technology translation previously acquired through courses to the HST health care providers were the key in controlling the first influenza A (H1N1) 2009 cases.
Diarrheagenic Escherichia coli in sub-Saharan Africa: Status, Uncertainties and Necessities
Authors: Okeke Iruka N 
Published: January 2010

Diarrhea is a leading cause of illness and death, particularly in developing countries. Enteropathogenic Escherichia coli, enterotoxigenic E. coli (ETEC), enteroinvasive E. coli, enterohemorrhaghic E. coli (EHEC), enteroaggregative E. coli (EAEC) and diffusely adherent E. coli (DAEC) have been associated with diarrheal disease in different parts of Africa, particularly among young children, HIV-positive individuals, and visitors from abroad. Each of these E. coli pathotypes uses distinct mechanisms, which are only partially understood, to colonize infected hosts and produce diarrhea. All known diarrheagenic E. coli pathotypes have been reported from diverse locations within Africa but the true burden from these pathogens is unknown because very few studies seeking these organisms with discriminatory methodology have been performed. Recent reports implicate ETEC and EAEC in a considerable proportion of childhood and travellers' diarrheas and suggest that some sub-types of these categories may have greater epidemiological significance than others. The significant contribution of EHEC to bloody diarrhea and hemolytic uremic syndrome is underappreciated because diagnostic capacity for this pathotype is generally inferior to that for confounders such as Shigella and Entamoeba. Recent studies in Africa have revealed the worrisome emergence of antimicrobial resistance and high asymptomatic carriage rates for diarrheagenic E. coli but bacterial and host factors that predispose to disease, as well as non-human reservoirs, are largely unknown. Future diarrheal disease research needs to focus on broadening the repertoire of pathogens sought in epidemiological surveys to include multiple categories of diarrheagenic E. coli while building capacity to detect these pathogens in local reference laboratories.
Use of flexible bronchoscopy for rapid diagnosis of suspected tubercular cases in rural India
Authors: Singhal Sameer , Gaidhane Abhay M. , Khatib Nazli , Hrivastava Tripti , Diwan Sanjay , Mahajan Satish , Bawankule Shilpa , Zahiruddin Quazi Syed 
Published: January 2010

Background: Reaching a correct diagnosis is a challenge for physicians treating any of the 30% to 50% of pulmonary tuberculosis patients who have negative sputum cultures or who present with no sputum. Flexible bronchoscopy acquires special importance for these cases for whom empirical anti-tuberculosis therapy is the only option left. In our study we aimed to assess the diagnostic yield of flexible bronchoscopy in patients, suspected to have tuberculosis, whose sputum smears were negative. Methodology: In our hospital-based cross-sectional study, 42 patients were enrolled by consecutive sampling. Flexible bronchoscopy and selective bronchial washings were done in all patients. Results: Bronchoscopy lavage smears were positive for M. tuberculosis in 10 (23.8%) patients. Fifteen (35.7%) patients had positive culture. Conclusion: Flexible bronchoscopy has an important role in the diagnosis of patients suspected to have tuberculosis, whose sputum smears are negative or who can not produce sputum.
Trends in antimicrobial susceptibility of Gram-negative bacteria isolated from blood in Jakarta from 2002 to 2008
Authors: Moehario Lucky H. , Tjoa Enty , Kiranasari Ariyani , Ningsih Ika , Rosana Yeva , Karuniawati Anis 
Published: January 2010

Background: This study examined the susceptibility of Gram-negative bacteria in the bloodstream to antimicrobials with the aim of providing information relevant to the guidance of therapy. Methodology: Blood specimens received by the Laboratory of Clinical Microbiology, Faculty of Medicine, University of Indonesia, from 2002 to 2008, were analyzed for the presence of Gram-negative bacteria and their susceptibility to four antibiotic groups frequently administered in hospitals and community settings. Results: During the seven-year period leading up to 2008, approximately 68% of Gram-negative bacteria were identified among all positive isolates from blood specimens. The eight most frequent species found were Acinetobacter anitratus (25.8%), Pseudomonas aeruginosa (19.5%), Klebsiella pneumoniae subsp. pneumoniae (14.5%), Enterobacter aerogenes (8%), Salmonella Typhi (7.5%), Escherichia coli (6.2%), Alcaligenes faecalis (5.6%) and Klebsiella oxytoca (3.2%). At 80% susceptibility or greater, Ceftriaxone and Cefotaxime were active only on E. coli and S. Typhi. Cefepime demonstrated activity on all eight species tested except K. pneumonia while Amikacin showed activity against five species, A. faecalis, E. aerogenes, E. coli, K. pneumoniae subsp. pneumoniae and S. Typhi. Gentamycin was active against three species: E. aerogenes, K. oxytoca and S. Typhi. Ciprofloxacin and Levofloxacin significantly differed in their spectrum: while Ciprofloxacin was active against four of the eight species tested (E. aerogenes, E. coli, K. oxytoca, and S. Typhi ), Levofloxacin was similar to Cefepime and was active against all eight species except K. pneumoniae subsp. pneumonia. Conclusions: Since antimicrobials are broadly used in Jakarta, it is important that the information captured in this study be disseminated.
The Nucleotide-Binding Oligomerization Domain-Like Receptor NLRC5 Is Involved in IFN-Dependent Antiviral Immune Responses
Authors: Kuenzel Sven , Till Andreas , Winkler Michael , Häsler Robert , Lipinski Simone , Jung Sascha , Grötzinger Joachim , Fickenscher Helmut , Schreiber Stefan , Rosenstiel Philip 
Published: January 2010

Abstract
Nucleotide-binding oligomerization domain-like receptors (NLRs) are a group of intracellular proteins that mediate recognition of pathogen-associated molecular patterns or other cytosolic danger signals. Mutations in NLR genes have been linked to a variety of inflammatory diseases, underscoring their pivotal role in host defense and immunity. This report describes the genomic organization and regulation of the human NLR family member NLRC5 and aspects of cellular function of the encoded protein. We have analyzed the tissue-specific expression of NLRC5 and have characterized regulatory elements in the NLRC5 promoter region that are responsive to IFN-γ. We show that NLRC5 is upregulated in human fibroblasts postinfection with CMV and demonstrate the role of a JAK/STAT-mediated autocrine signaling loop involving IFN-γ. We demonstrate that overexpression and enforced oligomerization of NLRC5 protein results in activation of the IFN-responsive regulatory promoter elements IFN-γ activation sequence and IFN-specific response element and upregulation of antiviral target genes (e.g., IFN-α, OAS1, and PRKRIR). Finally, we demonstrate the effect of small interfering RNA-mediated knockdown of NLRC5 on a target gene level in the context of viral infection. We conclude that NLRC5 may represent a molecular switch of IFN-γ activation sequence/IFN-specific response element signaling pathways contributing to antiviral defense mechanisms.
Physiological Numbers of CD4+ T Cells Generate Weak Recall Responses Following Influenza Virus Challenge
Authors: Thomas Paul G. , Brown Scott A. , Morris Melissa Y. , Yue Wen , So Jenny , Reynolds Cory , Webby Richard J. , Doherty Peter C. 
Published: January 2010

AbstractNaive and recall CD4+ T cell responses were probed with recombinant influenza A viruses incorporating the OVA OT-II peptide. The extent of OT-II–specific CD4+ T cell expansion was greater following primary exposure, with secondary challenge achieving no significant increase in numbers, despite higher precursor frequencies. Adoptive transfer experiments with OT-II TCR-transgenic T cells established that the predominant memory set is CD62Lhi, whereas the CD62Llo precursors make little contribution to the recall response. Unlike the situation described by other investigators, in which the transfer of very large numbers of in vitro-activated CD4 effectors can modify the disease process, providing CD62Lhi or CD62Llo OT-II–specific T cells at physiological levels neither enhanced virus clearance nor altered clinical progression. Some confounding effects of the transgenic model were observed, with decreasing primary expansion efficiency correlating with greater numbers of transferred cells. This was associated with increased levels of mRNA for the proapoptotic molecule Bim in cells recovered following high-dose transfer. However, even with very low numbers of transferred cells, memory T cells did not expand significantly following secondary challenge. A similar result was recorded in mice primed and boosted to respond to an endogenous IAb-restricted epitope derived from the influenza virus hemagglutinin glycoprotein. Depletion of CD8+ T cells during secondary challenge generated an increased accumulation of OT-II–specific T cells but only at the site of infection. Taken together, significant expansion was not a feature of these secondary influenza-specific CD4 T cell responses and the recall of memory did not enhance recovery.
Pharmacologic Inhibition of MEK–ERK Signaling Enhances Th17 Differentiation
Authors: Tan Andy Hee-Meng , Lam Kong-Peng 
Published: January 2010

Abstract
The cytokines and transcription factors that promote Th17 cell development have been extensively studied. However, the signaling pathways that antagonize Th17 differentiation remain poorly characterized. In this study, we report that pharmacologic inhibition of MEK–ERK signaling enhances the in vitro differentiation of Th17 cells and increases their gene expression of il-17a, il-17f, il-21, il-22, and il-23r. IL-2, which suppresses Th17 differentiation via STAT5 activation, also acts through ERK signaling to inhibit Th17 generation. In turn, ERK signaling is found to potentiate the production of IL-2 and activate STAT5, suggesting the existence of an autoregulatory loop to constrain Th17 development. Finally, compared with the transfer of untreated Th17 cells, the transfer of ERK-inhibited Th17 cells leads to accelerated onset and exacerbated colitis in immunodeficient mice. Our data indicate that MEK–ERK signaling negatively regulates Th17 differentiation in a Th cell-intrinsic manner.
IL-24 Transgenic Mice: In Vivo Evidence of Overlapping Functions for IL-20, IL-22, and IL-24 in the Epidermis
Authors: He Miao , Liang Peng 
Published: January 2010

Abstract
IL-20 and IL-24 share two different heterodimeric receptors consisting of either IL-20R1 or IL-22R1 and a common IL-20R2 subunit, whereas IL-22 signals through IL-22R1/IL-10R2. However, until now, only IL-20 and IL-22 have been proven to play important roles in vivo in the epidermis where all four receptor subunits are expressed. In this study, we show that IL-24 transgenic mice manifest many similar phenotypes to that of IL-20 and IL-22, including neonatal lethality, epidermal hyperplasia, and abnormality in keratinocyte differentiation. These results support a largely redundant role in epidermal functions for IL-20, IL-22, and IL-24, which seem to be IL-22R1 dependent. Moreover, we show that IL-24 transgenic mice exhibit infiltrating macrophages in the dermis with concomitant increases in MCP-1 production from both keratinocytes in the epidermis and immune infiltrates in the adjacent dermal layer below. Furthermore, we demonstrate that the homodimeric IL-20R2 soluble receptor is a potent blocker for IL-24 and can be used to further dissect the crosstalk among the IL-20 family of cytokines in normal development as well as in autoimmune diseases.
Characterization of IL-17A–Producing Cells in Celiac Disease Mucosa
Authors: Monteleone Ivan , Sarra Massimiliano , Del Vecchio Blanco Giovanna , Paoluzi Omero Alessandro , Franzè Eleonora , Fina Daniele , Fabrizi Alessia , MacDonald Thomas T. , Pallone Francesco , Monteleone Giovanni 
Published: January 2010

Abstract
Celiac disease (CD) is a gluten-sensitive enteropathy associated with a marked infiltration of the mucosa with IFN-γ–secreting Th1 cells. Recent studies have shown that a novel subset of T cells characterized by expression of high levels of IL-17A, termed Th17 cells, may be responsible for pathogenic effects previously attributed to Th1 cells. In this study, we characterized the expression of IL-17A–producing cells in CD. By real-time PCR and ELISA, it was shown that expression of IL-17A RNA and protein is more pronounced in active CD biopsy specimens in comparison with inactive CD and normal mucosal biopsy specimens. Flow cytometry confirmed that IL-17A is overproduced in CD mucosa and that CD4+ and CD4+CD8+ cells were major sources. The majority of IL-17A–producing CD4+ and CD4+CD8+ cells coexpressed IFN-γ but not CD161. The addition of a peptic‑tryptic digest of gliadin to ex vivo organ cultures of duodenal biopsy specimens taken from inactive CD patients enhanced IL-17A production by both CD4+ and CD4+CD8+ cells. Because we previously showed that IL-21, a T cell-derived cytokine involved in the control of Th17 cell responses, is overproduced in CD, we next assessed whether IL-17A expression is regulated by IL-21. Blockade of IL-21 activity by a neutralizing IL-21 Ab reduced IL-17A expression in cultures of active CD and peptic–tryptic digest of gliadin-treated CD biopsy specimens. In conclusion, our data show that IL-17A is increased in CD and is produced by cells that also make IFN-γ.
HIV Protective KIR3DL1 and HLA-B Genotypes Influence NK Cell Function Following Stimulation with HLA-Devoid Cells
Authors: Boulet Salix , Song Rujun , Kamya Philomena , Bruneau Julie , Shoukry Naglaa H. , Tsoukas Christos M. , Bernard Nicole F. 
Published: January 2010

Abstract
Epidemiological studies in humans have implicated carriage of combinations of genes encoding certain KIR3DL1 (killer Ig-like receptor 3DL1) alleles and their HLA-Bw4 ligands in slower progression to AIDS, lower viral load and protection from infection. Given that the KIR3DL1*h/*y/HLA-B*57 genetic combination is strongly associated with favorable HIV outcomes, we measured responses from NK cells isolated from these individuals by multiparametric flow cytometry for cytokine secretion and degranulation in response to stimulation with HLA-devoid cells to assess whether the KIR/HLA compound genotypes linked to better HIV outcome favor increased NK cell functional potential. Our results indicate that NK cells from these individuals had increased functional potential, particularly in the KIR3DL1+ NK cell subset. These results support a link between KIR/HLA genotypes and NK cell function and could provide an explanation for the observation that some KIR/HLA combinations are associated protective phenotypes in the context of host-HIV interactions.
IL-12 Suppresses Vascular Endothelial Growth Factor Receptor 3 Expression on Tumor Vessels by Two Distinct IFN-γ–Dependent Mechanisms
Authors: Sorensen Elizabeth W. , Gerber Scott A. , Frelinger John G. , Lord Edith M. 
Published: January 2010

Abstract
IL-12 has been shown to be effective in enhancing antitumor responses. However, how IL-12 exerts its antiangiogenic effect is largely unknown. In this study, we elucidate this mechanism using B16 transfected to express IL-12 (B16/IL-12), a system that provides constant, local production of IL-12 within the tumor microenvironment. Intratumoral IL-12 resulted in a significant delay in tumor growth and phenotypic changes in the vasculature. Vessels found within B16 tumors are chaotic and poorly formed and express vascular endothelial growth factor receptor 3 (VEGFR3), a growth factor receptor not expressed on normal adult vessels. However, the vessels within B16/IL-12 tumors have a more normal morphology and do not express VEGFR3. We have shown that IFN-γ is required for IL-12 to suppress the aberrant expression of VEGFR3. Indeed, the presence of intratumoral IL-12 stimulates the immune system resulting in more IFN-γ–producing tumor-infiltrating lymphocytes per tumor when compared with parental B16 tumors, which may have a marked effect on control of tumor growth. Interestingly, within B16/IL-12 tumors, T cells are necessary to suppress VEGFR3 expression on tumor vessels. Finally, using IFN-γ receptor knockout mice in a bone marrow chimera system, we show that the IFN-γ produced within the tumor suppresses VEGFR3 expression in two ways: 1) acting directly on tumor vessel endothelial cells, and 2) acting on the tumor-infiltrating lymphocytes to indirectly alter endothelial cells’ VEGFR3 expression. Our data indicate a mechanism in which tumor-infiltrating immune cells regulate tumor vessel phenotype.
Cutting Edge: Spontaneous Development of IL-17–Producing γδ T Cells in the Thymus Occurs via a TGF-β1–Dependent Mechanism
Authors: Do Jeong-su , Fink Pamela J. , Li Lily , Spolski Rosanne , Robinson Janet , Leonard Warren J. , Letterio John J. , Min Booki 
Published: January 2010

Abstract
In naive animals, γδ T cells are innate sources of IL-17, a potent proinflammatory cytokine mediating bacterial clearance as well as autoimmunity. However, mechanisms underlying the generation of these cells in vivo remain unclear. In this study, we show that TGF-β1 plays a key role in the generation of IL-17+ γδ T cells and that it mainly occurs in the thymus particularly during the postnatal period. Interestingly, IL-17+ γδ TCR+ thymocytes were mainly CD44highCD25low cells, which seem to derive from double-negative 4 γδ TCR+ cells that acquired CD44 and IL-17 expression. Our findings identify a novel developmental pathway during which IL-17–competent γδ T cells arise in the thymus by a TGF-β1–dependent mechanism.
Encapsulation of palladium nanoparticles by multiwall carbon nanotubes‐graft‐poly(citric acid) hybrid materials
Authors: Adeli Mohsen , Mehdipour Ebrahim , Bavadi Masoumeh 
Published: January 2010

AbstractCitric acid was polymerized onto the surface of functionalized multiwall carbon nanotubes (MWCNT‐COOH) and MWCNT‐graft‐poly(citric acid) (MWCNT‐g‐PCA) hybrid materials were obtained. Due to the grafted poly(citric acid) branches, MWCNT‐g‐PCA hybrid materials not only were soluble in water but also were able to trap water soluble metal ions. Reduction of trapped metal ions in the polymeric shell of MWCNT‐g‐PCA hybrid materials by reducing agents such as sodium borohydride led to encapsulated metal nanoparticles on the surface of MWCNT. Herein palladium nanoparticles were encapsulated and transported by MWCNT‐g‐PCA hybrid materials (MWCNT‐g‐PCA‐EPN) and their application as nanocatalyst toward Heck reaction in different conditions was investigated. The catalytic activity of palladium ions supported by MWCNT‐g‐PCA hybrid materials (MWCNT‐g‐PCA‐PdCl2) toward Heck reactions is much more than for MWCNT‐g‐PCA‐EPN. Structure, characteristics and catalytic activity of synthesized systems was investigated using spectroscopy and microscopy methods. © 2010 Wiley Periodicals, Inc. J Appl Polym Sci, 2010
New event detection and topic tracking in Turkish
Authors: Can Fazli , Kocberber Seyit , Baglioglu Ozgur , Kardas Suleyman , Ocalan H. Cagdas , Uyar Erkan 
Published: January 2010

AbstractTopic detection and tracking (TDT) applications aim to organize the temporally ordered stories of a news stream according to the events. Two major problems in TDT are new event detection (NED) and topic tracking (TT). These problems focus on finding the first stories of new events and identifying all subsequent stories on a certain topic defined by a small number of sample stories. In this work, we introduce the first large‐scale TDT test collection for Turkish, and investigate the NED and TT problems in this language. We present our test‐collection‐construction approach, which is inspired by the TDT research initiative. We show that in TDT for Turkish with some similarity measures, a simple word truncation stemming method can compete with a lemmatizer‐based stemming approach. Our findings show that contrary to our earlier observations on Turkish information retrieval, in NED word stopping has an impact on effectiveness. We demonstrate that the confidence scores of two different similarity measures can be combined in a straightforward manner for higher effectiveness. The influence of several similarity measures on effectiveness also is investigated. We show that it is possible to deploy TT applications in Turkish that can be used in operational settings.
Impacts of formaldehyde photolysis rates on tropospheric chemistry
Authors: Cooke M. C. , Utembe S. R. , Carbajo P. Gorrotxategi , Archibald A. T. , Orr‐Ewing A. J. , Jenkin M. E. , Derwent R. G. , Lary D. J. , Shallcross D. E. 
Published: January 2010

AbstractA global chemistry transport model is employed to investigate the impact of recent laboratory determinations of photolysis parameters for formaldehyde on concentrations of tropospheric trace gases. Using the new laboratory data, the photolysis of formaldehyde is a more significant removal pathway. HOx levels are increased with the greatest changes towards the top of the troposphere and the poles, making formaldehyde a more significant source of upper tropospheric HOx than previously thought. Global totals of ozone and secondary organic aerosol increase with the rise in ozone being more significant at higher solar zenith angles. Copyright © 2010 Royal Meteorological Society
Treatment of oesophageal anastomotic leaks by temporary stenting with self-expanding plastic stents (Br J Surg 2009; 96: 887–891)
Authors: Krishnan A , Robertson A G N , Dunn L J , Robinson S , Hayes N , Griffin S M 
Published: January 2010

Abstract
The Editors welcome topical correspondence from readers relating to articles published in the Journal. Responses should be sent electronically via the BJS website (www.bjs.co.uk). All letters will be reviewed and, if approved, appear on the website. A selection of these will be edited and published in the Journal. Letters must be no more than 250 words in length.