bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nupr1: The Swiss‐knife of cancer
Authors: Cano Carla E. , Hamidi Tewfik , Sandi Maria José , Iovanna Juan L. 
Published: July 2010

AbstractNupr1 is a small, highly basic and loosely folded multifunctional protein whose expression is induced by several stresses. Its relation to cancer was first suggested by its overexpression in several human malignancies and the association of its expression with breast cancer metastasis. Accordingly, Nupr1 is structurally related to the high‐mobility group (HMG) of transcriptional regulators, which play a key role in the stress response and in cancer progression. Nupr1 interacts with numerous partners to regulate cell cycle, programmed cell death, autophagy, chromatin accessibility, and transcription, and its expression is required for regulation of TGFβ activity. Pleiotropic functions accomplished by Nupr1 depend on its molecular partners, its location into the cell, its expression level and on the cell‐type. Nupr1 might be a new drug‐targetable protein whose blockade would prevent cancer progression and metastasis development. J. Cell. Physiol. 226: 1439–1443, 2011. © 2010 Wiley‐Liss, Inc.
Identification of human placenta‐derived mesenchymal stem cells involved in re‐endothelialization
Authors: Tran Tu Cam , Kimura Kenichi , Nagano Masumi , Yamashita Toshiharu , Ohneda Kinuko , Sugimori Haruhiko , Sato Fujio , Sakakibara Yuzuru , Hamada Hiromi , Yoshikawa Hiroyuki , Hoang Son Nghia , Ohneda Osamu 
Published: July 2010

AbstractHuman placenta is an attractive source of mesenchymal stem cells (MSC) for regenerative medicine. The cell surface markers expressed on MSC have been proposed as useful tools for the isolation of MSC from other cell populations. However, the correlation between the expression of MSC markers and the ability to support tissue regeneration in vivo has not been well examined. Here, we established several MSC lines from human placenta and examined the expression of their cell surface markers and their ability to differentiate toward mesenchymal cell lineages. We found that the expression of CD349/frizzled‐9, a receptor for Wnt ligands, was positive in placenta‐derived MSC. So, we isolated CD349‐negative and ‐positive fractions from an MSC line and examined how successfully cell engraftment repaired fractured bone and recovered blood flow in ischemic regions using mouse models. CD349‐negative and ‐positive cells displayed a similar expression pattern of cell surface markers and facilitated the repair of fractured bone in transplantation experiments in mice. Interestingly, CD349‐negative, but not CD349‐positive cells, showed significant effects on recovering blood flow following vascular occlusion. We found that induction of PDGFβ and bFGF mRNAs by hypoxia was greater in CD349‐negative cells than in CD349‐positive cells while the expression of VEGF was not significantly different in CD349‐negative and CD349‐positive cells. These findings suggest the possibility that CD349 could be utilized as a specialized marker for MSC isolation for re‐endothelialization. J. Cell. Physiol. 226: 224–235, 2010. © 2010 Wiley‐Liss, Inc.
A scanning electron microscopy study of the embryonic development of Pycnogonum litorale (Arthropoda, Pycnogonida)
Authors: Machner Jakob , Scholtz Gerhard 
Published: July 2010

AbstractThe phylogenetic position of the enigmatic Pycnogonida (sea spiders) is still controversial. This is in part due to a lack of detailed data about the morphology and ontogenesis of this, in many aspects, aberrant group. In particular, studies on the embryonic development of pycnogonids are rare and in part contradictory. Here, we present the first embryological study of a pycnogonid species using scanning electron microscopy (SEM). We describe the late embryogenesis of Pycnogonum litorale from the first visible appendage anlagen to the hatchling in 11 embryonic stages. The three pairs of appendage anlagen gain in length by growth, as well as by extension of furrows into the embryo. The opening of the stomodaeum is located far in front of the anlagen of the chelifores and has a Y‐shaped lumen from the onset. During further embryogenesis, the position of the mouth shifts ventrally, until it is located between the chelifores. The proboscis anlage grows out as a circumoral wall‐like structure, which is initially more pronounced ventrally. Hypotheses about the evolution of the proboscis by fusion of originally separated components are critically discussed, because the proboscis anlage of P. litorale shows no indications of a composite nature. In particular, a participation of post‐cheliforal elements in proboscis formation is rejected by our data. Further, no preoral structure and no stage in proboscis formation was found, which could plausibly be homologized with the labrum of othereuarthropods. Thus, our study supports the assumption of a complete lack of a labrum in Pycnogonida. J. Morphol., 2010. © 2010 Wiley‐Liss, Inc.
Liquid chromatography on a monolithic column microfluidic chip coupled with “three‐T” sample injection mode and amperometric detection
Authors: Wang ZongWen , Wang WenJian , Chen GuoNan , Wang Wei , Fu FengFu 
Published: July 2010

AbstractAn LC microfluidic chip (LC chip) with amperometric detection was developed. The LC chip employed a 7.5 cm long reversed‐phase polymethacrylate monolithic column as a stationary phase and a “three‐T” injection mode. A convenient interface was designed to conduct pump pressure into the microfluidic chip to drive solution, and a home‐made device was used to control the distance between the working electrode and the LC chip accurately. The “three‐T” sample injection mode completely avoided the problem of sample dilution and sample leakage during separation, which is usually observed in traditional and “T” type LC chip, without the using of valve and finally results in a better resolution, reproducibility and relatively high sensitivity. Using the proposed LC chip system, we have successfully separated two isomers, catechol and hydroquinone, within 12 min with a RSD (n=3) <3.0% for retention time and <2.4% for peak area. We have also successfully separated and determined 5‐hydroxy‐L‐tryptophan, dopamine and 5‐hydroxytryptamine within 25 min with a RSD (n=3) <5% (for peak area) and a detection limit of 0.16–0.51 μmol/L.
A comprehensive LC‐MS‐based quantitative analysis of fentanyl‐like drugs in plasma and urine
Authors: Cooreman Sarah , Deprez Christa , Martens Frank , Van Bocxlaer Jan , Croes Kathleen 
Published: July 2010

AbstractFentanyl, norfentanyl, alfentanil, sufentanil, remifentanil and 3‐methylfentanyl are potent, short‐acting, synthetic narcotic analgesics that are not revealed in standard opiate immunoassays. In this article, a fully validated analytical method for the determination of these fentanyl‐type compounds in plasma and urine is presented, consisting of a liquid–liquid extraction followed by a LC‐MS/MS analysis using electrospray ionisation in the positive ionisation mode. Fentanyl‐d5 and norfentanyl‐d5 were used as internal standards. The lower LOQ in plasma and urine was 0.1 ng/mL for fentanyl, norfentanyl, alfentanil, remifentanil and 3‐methylfentanyl, and 0.2 ng/mL for sufentanil. The method proved linear over a concentration range of 0.2–50 ng/mL for sufentanil and 0.1–50 ng/mL for all other analytes, with correlation coefficients of 0.998 or better. The analytical procedure showed excellent selectivity and precision (all CVs below 15%) for all analytes. Accuracy was good, except for sufentanil, where deviations of more than 15% from nominal concentrations were observed. No matrix effects were observed, and stability of stock and internal standard solutions was within acceptability limits.
Analysis of contributing factors to production of highly transparent isotactic polypropylene extrusion sheets. Part I
Authors: Funaki Akira , Kanai Toshitaka , Saito Yuta , Yamada Toshiro 
Published: July 2010

AbstractTo obtain highly transparent isotactic polypropylene sheet by an industrial process, various factors contributing to transparency were analyzed. Two kinds of sheets were prepared for analysis. The one was extruded by a direct single belt process as the quick quenching system. And the other was extruded by a conventional air knife process as the slow cooling system. The higher order structure of each sample sheet was investigated by phase‐contrast microscopy, polarizing microscopy, light scattering, wide‐angle X‐ray diffraction (WAXD), small angle X‐ray scattering (SAXS), and transmission electron microscope (TEM). In the case of the quick quenching sheet, many spherical substances were generated near sheet surfaces. Comparing with static cooling experiments, it was thought that the main cause was the orientation crystallization by the residual stress. The spherical substances in a phase‐contrast micrograph were extremely coincident with the maltese cross pattern in a polarizing micrograph. Other analysis results also showed that these spherical substances were spherulites. And after the heat treatment, the matrix was transformed from smectic structure to α‐monoclinic crystal phase. The density and refractive index differences between spherulite and matrix were decreased, and the transparency was drastically improved. In addition, some guideline for the improvement of transparency was indicated. POLYM. ENG. SCI., 2010. © 2010 Society of Plastics Engineers
Photoelectrochemical properties of porous silicon based novel photoelectrodes
Authors: Betty C A , Sasikala R , Jayakumar O D , Sakuntala T , Tyagi A K 
Published: July 2010

AbstractPorous silicon interfaces have been modified with nitrided TiO2 (TiON) nanoparticles to develop highly efficient photoelectrodes. Photoelectrodes were prepared by impregnating the electrochemically prepared porous silicon microchannels with titanium oxynitride. Photocatalytic measurements were carried out on titanium oxynitride particles in water‐methanol mixture and the results showed a dependence on the nitrogen concentration. Among the photoelectrodes used for photocurrent measurements, porous silicon impregnated with TiO2 nitrided at 600 °C showed maximum photocurrent increase after exposure to sunlight‐type radiation. The enhancement in photocurrent was one order more for the porous silicon/titanium oxynitride hetero‐structure than that of polished silicon/titanium oxynitride hetero‐structure. Photoelectrodes thus prepared were found to have stable performance for a period of six months. This observation promises the possibility of using porous silicon/titanium oxynitride hetero‐structures as efficient electrodes for photovoltaic cells. Copyright © 2010 John Wiley & Sons, Ltd.
Inhaled colistin for the treatment of tracheobronchitis and pneumonia in critically ill children without cystic fibrosis
Authors: Falagas Matthew E. , Sideri Georgia , Korbila Ioanna P. , Vouloumanou Evridiki K. , Papadatos John H. , Kafetzis Dimitris A. 
Published: July 2010

AbstractData regarding the role of inhaled colistin in critically ill pediatric patients without cystic fibrosis are scarce. Three children (one female), admitted to the intensive care unit (ICU) of a tertiary‐care pediatric hospital in Athens, Greece, during 2004–2009 received inhaled colistin as monotherapy for tracheobronchitis (two children), and as adjunctive therapy for necrotizing pneumonia (one child). Colistin susceptible Acinetobacter baumannii and Pseudomonas aeruginosa were isolated from the cases' bronchial secretions specimens. All three children received inhaled colistin at a dosage of 75 mg diluted in 3 ml of normal saline twice daily (1,875,000 IU of colistin daily), for a duration of 25, 32, and 15 days, respectively. All three children recovered from the infections. Also, a gradual reduction, and finally total elimination of the microbial load in bronchial secretions was observed during inhaled colistin treatment in the reported cases. All three cases were discharged from the ICU. No bronchoconstriction or any other type of toxicity of colistin was observed. In conclusion, inhaled colistin was effective and safe for the treatment of two children with tracheobronchitis, and one child with necrotizing pneumonia. Further studies are needed to clarify further the role of inhaled colistin in pediatric critically ill patients without cystic fibrosis. Pediatr Pulmonol. 2010;45:1135–1140. © 2010 Wiley‐Liss, Inc.
Variations in How Physiotherapists Understand Their Work on Organizational and Societal Levels
Authors: Boll Madeleine , Rosenqvist Urban 
Published: July 2010

AbstractBackground and Purpose. Many physiotherapists have moved from treating persons on individual and group levels to working in different service positions on organizational and societal levels within healthcare organizations. The present study has investigated how physiotherapists working on those levels understand their job. The aim of the study was to describe variations in physiotherapists' understanding of their work on organizational and societal levels within healthcare organizations. The research question was: how do physiotherapists understand their work on organizational and societal levels? Method. A phenomenographic approach was chosen. Physiotherapists working in different positions on organizational and societal levels were interviewed. Results. Among our 21 informants we found three logically interrelated categories: ‘exploring and performing an assignment’, ‘interactive learning and balancing support’ and ‘moving within a continually changing structure’. These three categories of descriptions formed an outcome space of ways of understanding work on organizational and societal levels within healthcare organizations. Conclusions. The findings in this study can be used as tools in professional discussions on how ways of understanding work have an impact on how work is performed and how change is achieved. Copyright © 2010 John Wiley & Sons, Ltd.
Protein–protein docking benchmark version 4.0
Authors: Hwang Howook , Vreven Thom , Janin Joël , Weng Zhiping 
Published: July 2010

AbstractWe updated our protein–protein docking benchmark to include complexes that became available since our previous release. As before, we only considered high‐resolution complex structures that are nonredundant at the family–family pair level, for which the X‐ray or NMR unbound structures of the constituent proteins are also available. Benchmark 4.0 adds 52 new complexes to the 124 cases of Benchmark 3.0, representing an increase of 42%. Thus, benchmark 4.0 provides 176 unbound–unbound cases that can be used for protein–protein docking method development and assessment. Seventeen of the newly added cases are enzyme‐inhibitor complexes, and we found no new antigen‐antibody complexes. Classifying the new cases according to expected difficulty for protein–protein docking algorithms gives 33 rigid body cases, 11 cases of medium difficulty, and 8 cases that are difficult. Benchmark 4.0 listings and processed structure files are publicly accessible at http://zlab.umassmed.edu/benchmark/ Proteins 2010. © 2010 Wiley‐Liss, Inc.
Social stress in young people with specific language impairment
Authors: Wadman Ruth , Durkin Kevin , Conti‐Ramsden Gina 
Published: July 2010

ABSTRACTSocial interactions can be a source of social stress for adolescents. Little is known about how adolescents with developmental difficulties, such as specific language impairment (SLI), feel when interacting socially. Participants included 28 adolescents with SLI and 28 adolescents with typical language abilities (TL). Self‐report measures of social stress, social skills and social acceptance were obtained. Participants with SLI reported experiencing significantly more social stress than did participants with TL. Both groups judged themselves as having adequate social skills and positive social acceptance. Expressive language ability was negatively associated with social stress, but did not predict social stress when social factors were included in the regression model. Perceived social skills and social acceptance scores predicted social stress, in that poorer scores predicted more social stress. Despite perceiving themselves as having adequate social skills and as being socially accepted, social interactions are nonetheless a source of stress for adolescents with SLI.
Mortality and employment after in-patient opiate detoxification
Authors: Naderi-Heiden A. , Gleiss A. , Bäcker C. , Bieber D. , Nassan-Agha H. , Kasper S. , Frey R. 
Published: July 2010

AbstractAimWe considered that completed opiate detoxification resulted in increased life expectancy and earning capacity as compared to non-completed detoxification.MethodsThe cohort study sample included pure opioid or poly-substance addicts admitted for voluntary in-patient detoxification between 1997 and 2004. Of 404 patients, 58.7% completed the detoxification program and 41.3% did not. The Austrian Social Security Institution supplied data on survival and employment records for every single day in the individual observation period between discharge and December 2007. Statistical analyses included the calculation of standardized mortality rates for the follow-up period of up to 11 years.ResultsThe standardized mortality ratios (SMRs) were between 13.5 and 17.9 during the first five years after discharge, thereafter they fell clearly with time. Mortality did not differ statistically significantly between completers and non-completers. The median employment rate was insignificantly higher in completers (12.0%) than in non-completers (5.5%). The odds for being employed were higher in pure opioid addicts than in poly-substance addicts (p = 0.003).ConclusionsThe assumption that completers of detoxification treatment have a better outcome than non-completers has not been confirmed. The decrease in mortality with time elapsed since detoxification is interesting. Pure opioid addicts had better employment prospects than poly-substance addicts.
Somatocognitive therapy in the management of chronic gynaecological pain. A review of current approach and historical background
Authors: Haugstad Gro Killi , Kirste Unni , Leganger Siv , Haakonsen Elin , Haugstad Tor S 
Published: July 2010

Abstract

Introduction
We have developed somatocognitive therapy as a hybrid of Mensendieck physiotherapy and cognitive psychotherapy. Womenwith chronic pelvic pain (CPP) and vulvodynia (chronic pain of the vulvae and vestibulum, VD) were recruited into two separate treatment protocols as described.

Methods
60 patients with CPP were recruited from the Department of Gynaecology at the Oslo University Hospital into a randomized, controlled intervention study. The patients were randomized into three treatment groups, receiving (1) treatment as usual, (2) somatocognitive therapy, and (3) in addition receiving cognitive therapy. The patients were assessed by means of SMT, Visual Analogue Score of Pain (VAS), and General Health Questionnaire (GHQ-30) at baseline, after three months of out-patient therapy and at 1 year follow-up. 9 women with VD were treated in an outpatient setting by physiotherapy students under senior supervision, each receiving in all 16 therapy sessions over 8 weeks, and scored for motor patterns (SMT) and pain (VAS) before and after therapy.

Results
In the control group, no significant change was found. In the group receiving somatocognitive therapy, significant reduction in pain score and improvement in motor function were found the end of therapy, and the significant improvement continued through the follow-up period. GHQ scores were significantly improved for the scores representing level of anxiety and coping, and improved for depression. In the group receiving cognitive therapy in addition, the VAS scores were reduced to the same level as the group receiving only somatocognitive therapy, whereas the motor patterns showed slightly less improvement than for those women that did not receive cognitive intervention. In the women with VD somatocognitive therapy resulted in significantly reduction in pain scores (by an average of 66%), and significant improvement of motor patterns, especially for the scores for gait (56%) and respiration (88%).

Conclusions
Somatocognitive therapy is a new approach that appears to be very promising in the management of chronic gynaecological pain.
Short-term out-patient treatment significantly reduces pain scores and improves motor function, especially with respect to respiration, gait and movement (ability to relax).
The approach is now being used in a randomized, controlled intervention study including patient with chronic low back, neck and shoulder, and widespread pain.
Predicting of pain, disability, and sick leave regarding a non-clinical sample among Swedish nurses
Authors: Nilsson Annika , Lindberg Per , Denison Eva 
Published: July 2010

Abstract

Objective
Health care providers, especially registered nurses (RNs), are a professional group with a high risk of musculoskeletal pain (MSP). This longitudinal study contributes to the literature by describing the prevalence and change in MSP, work-related factors, personal factors, self-reported pain, disability and sick leave (>7 days) among RNs working in a Swedish hospital over a 3-year period. Further, results concerning prediction of pain, disability and sick leave from baseline to a 3-year follow-up are reported.

Method
In 2003, a convenience sample of 278 RNs (97.5% women, mean age 43 years) completed a questionnaire. In 2006, 244 RNs (88% of the original sample) were located, and 200 (82%) of these completed a second questionnaire.

Results
Logistic regression analyses revealed that pain, disability and sick leave at baseline best predicted pain, disability, and sick leave at follow-up. The personal factors self-rated health and sleep quality during the last week predicted pain at follow-up, while age, self-rated health, and considering yourself as optimist or pessimist predicted disability at follow-up, however weakly. None of the work-related factors contributed significantly to the regression solution.

Conclusions
The results support earlier studies showing that a history of pain and disability is predictive of future pain and disability. Attention to individual factors such as personal values may be needed in further research.
A theory of MHD instability of an inhomogeneous plasma jet
Authors: LEONOVICH ANATOLY S. 
Published: July 2010

AbstractA problem of the stability of an inhomogeneous axisymmetric plasma jet in a parallel magnetic field is solved. The jet boundary becomes, under certain conditions, unstable relative to magnetosonic oscillations (Kelvin–Helmholtz instability) in the presence of a shear flow at the jet boundary. Because of its internal inhomogeneity the plasma jet has resonance surfaces, where conversion takes place between various modes of plasma magnetohydrodynamic (MHD) oscillations. Propagating in inhomogeneous plasma, fast magnetosonic waves drive the Alfven and slow magnetosonic (SMS) oscillations, tightly localized across the magnetic shells, on the resonance surfaces. MHD oscillation energy is absorbed in the neighbourhood of these resonance surfaces. The resonance surfaces disappear for the eigenmodes of SMS waves propagating in the jet waveguide. The stability of the plasma MHD flow is determined by competition between the mechanisms of shear flow instability on the boundary and wave energy dissipation because of resonant MHD-mode coupling. The problem is solved analytically, in the Wentzel, Kramers, Brillouin (WKB) approximation, for the plasma jet with a boundary in the form of a tangential discontinuity over the radial coordinate. The Kelvin–Helmholtz instability develops if plasma flow velocity in the jet exceeds the maximum Alfven speed at the boundary. The stability of the plasma jet with a smooth boundary layer is investigated numerically for the basic modes of MHD oscillations, to which the WKB approximation is inapplicable. A new 'unstable mode of MHD oscillations has been discovered which, unlike the Kelvin–Helmholtz instability, exists for any, however weak, plasma flow velocities.