bluuebunny/crossref_metadata_2025_split huggingface dataset:
Japanese spotted fever with acute hepatic failure: was it associated with Epstein‐Barr virus?
Authors: Kondo Makoto , Kurokawa Ichiro , Yamanaka Kei‐ichi , Akachi Shigehiro , Nishii Masami 
Published: November 2010

AbstractBackground An 81‐year‐old female experiencing high fever, fatigue, and loss of appetite was admitted to our hospital and diagnosed with acute cholecystitis. Her condition did not improve and an eschar and erythema subsequently appeared. We then diagnosed Japanese spotted fever (JSF). She recovered immediately after the administration of minocycline. This case differed from other cases because the patient had a remarkably acute hepatic failure.Methods Considering that the present case might be associated with other factors, we performed a repeat polymerase chain reaction (PCR) test on the patient's blood that had been collected on admission and stored.Results Epstein‐Barr virus (EBV) was detected in her blood by PCR.Conclusion We consider this case might be associated with EBV.
Cutaneous endometriosis
Authors: Fernández Vozmediano J. M. , Armario Hita J. C. , Cuevas Santos J. 
Published: November 2010

AbstractA 32‐year old patient presented with an asymptomatic, non‐specific tumour located at the site of a previous cesarian scar. The tumor had never bled but its size changed and the color and consistency varied coinciding with the menstrual cycle. The dermatopathologic study showed the existence of ectopic endometrial glandular tissue in the whole thickness of the reticular dermis.
HLA‐DR alleles associated with skin warts induced by human papillomavirus infection
Authors: García‐Corona Cristina , Vega‐Memije Elisa , Barquera Rodrigo , Granados Julio 
Published: November 2010

AbstractBackground  The skin wart is a benign proliferation of the skin and mucous, secondary to human papillomavirus (HPV) infection.Objectives  The objective of this study is to determine gene frequencies of HLA‐DR alleles in Mexican patients with skin warts and compare them with those present in ethnically matched healthy subjects.Methods  Fifty‐two patients with clinically and histologically confirmed skin warts from the Dermatology Outpatient Clinic, with results of high‐resolution DNA typing for HLA‐DR polymorphism.Results  HLA‐DR3 and DR9 were increased (P = 0.0029, OR: 2.5, 95% CI: 1.3–4.7 and P = 0.0062, OR: 5.4, 95% CI: 1.4–19.5, respectively), and HLA‐DR6 allele was found decreased (P = 0.0002).Limitations  The major histocompatibility complex contribution in the infection and elimination of the virus is not clear and perhaps also contributes to a series of events not well established yet.Conclusions  This study follows the preponderant role of class II genes in the susceptibility or resistance to the development of skin warts caused by HPV infection.
Topical nadifloxacin 1% cream vs. topical erythromycin 4% gel in the treatment of mild to moderate acne
Authors: Tunca Mustafa , Akar Ahmet , Ozmen Ibrahim , Erbil Hakan 
Published: November 2010

AbstractTopical antibiotics are the mainstay of therapy in mild to moderate inflammatory acne. Topical erythromycin is one of the most common prescribed topical antibiotics. Nadifloxacin, another topical antibiotic for acne, was recently introduced into the market in our country. In this study, we compared the efficacies and safety of topical nadifloxacin 1% cream and erythromycin 4% gel in acne. A total of 86 patients with mild to moderate facial acne were randomized into two treatment groups. The efficacies of the drugs were assessed by lesion counts. An acne severity index (ASI) was also calculated. In both groups, there was a significant reduction in lesion counts and ASI scores beginning from the first visit at week 4. This reduction continued throughout the 12‐week study period. Both treatments were well tolerated. We conclude that when topically applied, both nadifloxacin 1% cream and erythromycin 4% gel are equally effective and safe treatments for mild to moderate facial acne.
Cutaneous spindle cell squamous cell carcinoma in nevus sebaceous
Authors: Zhou‐Wei Wu , Wei‐min Shi , Yue Sun , Xiao‐Jie Li , Jun Song 
Published: November 2010

AbstractA case of a 28 year‐old Chinese male who presented with a rapidly growing tumor within a nevus sebaceous on his right cheek. The tumor was excised and immunohistochemical analysis and histology were consistent with spindle‐cell squamous cell carcinoma. To the best of our knowledge, it is the first report of cutaneous spindle‐cell squamous cell carcinoma developing in nevus sebaceous in the English literature. This report highlights the importance of early excision of any nevus sebaceous with a history of change.
Ritodrine‐induced erythematous papular eruption in 14 pregnant women
Authors: Sato Yoshihiro , Teraki Yuichi , Izaki Seiichi , Baba Kazunori 
Published: November 2010

AbstractBackground  Ritodrine hydrochloride, a β2‐adrenergic agonist, has been used for the treatment of pre‐term labor as a relatively safe agent, although tolerable side‐effects have been occasionally reported.Objective  The purpose of this study was to assess our clinical experience of skin eruptions caused by ritodrine.Methods  Fourteen pregnant women with pruritic skin eruptions associated with the administration of ritodrine for pre‐term labor were examined in Saitama Medical Center Hospital between 2005 and 2008.Results  Patients included both primigravidas and multigravidas. Their mean age was 33.7 years (range: 27–41 years). Almost all subjects were in the third trimester of pregnancy. Skin eruptions occurred 7–27 days (mean: 14.9 days) after the start of intravenous or oral ritodrine. In eight patients, the eruption occurred after an increase in the dose of the drug. The reaction was characterized by a pruritic, erythematous, papular eruption, mainly distributed on the abdomen and upper extremities. Lymphocyte transformation tests for ritodrine were positive in five of the eight patients.Conclusions  Ritodrine‐induced, erythematous, papular eruption probably occurs more frequently than has been previously estimated. An immunologic mechanism may play a role in the development of this eruption caused by ritodrine, although the reaction is somewhat dependent on the dose.
A Canadian online survey to evaluate awareness and treatment satisfaction in individuals with moderate to severe plaque psoriasis
Authors: Poulin Yves , Papp Kim A. , Wasel Norman R. , Andrew Robin , Fraquelli Elisa , Bernstein Geula , Chan Daphne 
Published: November 2010

AbstractBackground  Psoriasis is a chronic inflammatory disease associated with comorbidities and decreased quality of life. This survey is aimed to better understand the impact of disease on Canadian patients, and to examine awareness and use of available treatment options.Methods  An online survey was conducted using a consumer panel. Eligible subjects reported diagnoses of psoriasis and moderate/severe/very severe plaque psoriasis within the past 5 years, and either: psoriasis covering ≥ 3% of body surface area; psoriasis on a sensitive area, or current use of systemic and/or phototherapy or light therapy for psoriasis.Results  A total of 514 panelists completed the survey; 65% reported current moderate/severe/very severe psoriasis. Awareness of available treatment options ranged from 98% for prescription topical agents to 75% for photo/light therapy, and < 50% for prescription oral (49%) or injectable (35%) medications. A total of 92% of respondents had been treated with and 61% were currently taking prescription topical agents. Photo/light therapy had been used by 38% and was currently used by 7% of respondents. Prescribed oral medication had been taken by 25% and was currently used by 8%. Few subjects had been treated with injectables in the past (10%) or currently (5%). Overall, 24% of respondents were very satisfied with their current treatment. A total of 63% of respondents taking injectables were very satisfied, compared with 38% of those taking prescribed oral medication and 21% of those receiving photo/light therapy.Conclusions  Most respondents with moderate to severe psoriasis were unaware of all treatment options; systemic treatments were not commonly utilized. Treatment satisfaction rates were low, highlighting the need to ensure greater patient education on and use of available therapeutic options.
Vocal Repertoire of Captive Capybara (Hydrochoerus hydrochaeris): Structure, Context and Function
Authors: Barros Kamila S. , Tokumaru Rosana S. , Pedroza Janine P. , Nogueira Selene S. C. 
Published: November 2010

AbstractIn this work we describe the structure, behavioral context and functionality of the vocal repertoire of capybaras, a social Caviomorph rodent. Additionally, since territoriality is present in this species, we hypothesize the occurrence of vocal differences among social groups. We analyzed a total of 2069 calls emitted by 28 animals from three different social groups. The capybara’s repertoire is comprised by seven call types (whistle, cry, whine, squeal, bark, click and tooth‐chattering). The vocalizations were functionally categorized as contact, alarm, distress and agonistic calls considering their behavioral contexts. The click calls emitted by the adults of the three captive capybara groups were significantly different, confirming our hypothesis of social groups’ vocal differences. The richness of interactions mediated by vocalization in capybaras suggests that the species’ communication is an important mechanism to regulate social encounters and to alert members of the group about environmental cues.
Partial Foetal Retention Following Aglepristone Treatment in a Bitch
Authors: Rigau T , Rodríguez‐Gil J‐E , García F , del Alamo MM Rivera 
Published: November 2010

ContentsThis short communication describes the case of partial foetal retention in an 18‐month‐old female French bulldog following induction of abortion owing to an undesired mating. Abortion was induced with aglepristone administered in two consecutive protocols of a dual injection 1 day apart. After failure of the first treatment to achieve abortion, 15 days later, a second treatment was administered. Delivering of aborted foetus occurred 2 days after the last administration. Five weeks after the abortion, the female showed a weak haemorrhagic vaginal discharge. On ultrasound examination, the presence of uterine wall distension as well as a puppy skull inside the uterus was observed. This clinical case makes clear that although aglepristone is a very reliable drug, follow‐up of the female during treatment and in the immediate post‐partum is necessary to ensure a good outcome.
Calcium oxalate crystal deposition in metabolic syndrome model rat kidneys
Authors: Okamoto Masanori , Kohjimoto Yasuo , Iba Akinori , Saji Fumie , Hara Isao , Shigematsu Takashi 
Published: November 2010

Objective:  Although an epidemiological link between the metabolic syndrome and kidney stone formation has been reported, the mechanism by which metabolic syndrome promotes kidney stone formation has yet to be elucidated. We investigated calcium oxalate (CaOx) kidney stone formation in a rat metabolic syndrome model.Methods:  We induced hyperoxaluria in 8‐week‐old male Otsuka Long‐Evans Tokushima fatty (OLETF) rats, and a control strain, Long‐Evans Tokushima Otsuka (LETO) rats, by administering 1.0% ethylene glycol (EG) as their drinking water for 2 weeks. Rats were divided into four groups: LETO‐C (control, n = 7); LETO‐SF (stone forming, n = 8); OLETF‐C (n = 7); and OLETF‐SF (n = 8). Urine and blood samples were collected for biochemistry testing, and the kidneys were harvested for estimation of crystal deposition and determinations of the expression of osteopontin (OPN) and monocyte chemoattractant protein‐1 (MCP‐1).Results:  Administration of EG induced hyperoxaluria to the same degree in both strains. The OLETF‐SF group showed a higher grade of renal crystal deposition and significantly higher renal calcium content than the LETO‐SF group. Although the OLETF‐C group excreted significantly higher amounts of uric acid and more acidic urine than the LETO‐C group, similar differences were not observed in rats given EG. Significant upregulation of both OPN and MCP‐1 was seen in the kidneys of hyperoxaluric rats, with higher levels of expression in the OLETF‐SF group than the LETO‐SF group.Conclusions:  The present results show for the first time that OLETF rats form more renal CaOx crystal deposits compared with control rats under EG‐induced hyperoxaluric conditions. The model described here should be useful for investigating the mechanisms by which the metabolic syndrome promotes CaOx kidney stone formation.
Profilin 1 overexpression in renal cell carcinoma
Authors: Minamida Satoru , Iwamura Masatsugu , Kodera Yoshio , Kawashima Yusuke , Ikeda Masaomi , Okusa Hiroshi , Fujita Tetsuo , Maeda Tadakazu , Baba Shiro 
Published: November 2010

Objectives:  To gain information about overexpressed antigens in renal cell carcinoma (RCC) by using a chemical proteomics approach.Methods:  RCC cell line 769P was cultured and proteome analysis was subsequently carried out in the culture supernatants. By using sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (SDS‐PAGE) and tandem mass spectrometry (LC‐MS/MS), proteins in the culture supernatants were searched. A MEDLINE search to define the functions of the identified proteins was carried out.Results:  Four differentially regulated proteins (profilin 1, amyloid beta A4 protein [APP], proprotein convertase subtilisin/kexin type 1 inhibitor [ProSAAS], galectin‐3‐binding protein [LGALS3BP]) were selected. These were not overexpressed in normal kidney tissue or reported in RCC. Their levels were measured through western blotting of normal kidney and RCC tissues. No differences were observed in the expression levels of APP, ProSAAS or LGALS3BP between RCC and normal kidney tissues. Profilin 1 was overexpressed in RCC tissue. On the basis of this observation, an immunohistochemical analysis of profilin 1 in normal kidney and RCC tissues was carried out. In normal tissues, tubules that were sources of RCC stained positive for profilin 1. In RCC tissue, in contrast, the stromal cells in the tumors stained positive.Conclusions:  Profilin 1 can be a key element in the pathological processes of RCC, such as tumorigenesis and/or tumor growth. Thus, it has the potential to serve as a diagnostic or progression biomarker and therapeutic target in RCC.
Management of hypertrophic pylorus stenosis with ultrasound guided single shot epidural anaesthesia – a retrospective analysis of 20 cases
Authors: Willschke Harald , Machata Anette‐Marie , Rebhandl Winfried , Benkoe Thomas , Kettner Stephan C. , Brenner Lydia , Marhofer Peter 
Published: November 2010

SummaryAim:  To retrospectively describe the performance of ultrasound guided thoracic epidural anaesthesia under sedation for anaesthesia management of open pyloromyotomy.Background:  Anaesthesia management for hypertrophic pylorus stenosis (HPS) is usually performed under general anaesthesia with tracheal intubation. Only a few publications describe avoidance of tracheal intubation in infants by using spinal or caudal anaesthesia. The present retrospective analysis describes the performance of ultrasound guided thoracic epidural anaesthesia under sedation for anaesthetic management of open pyloromyotomy.Methods:  Twenty consecutive infants scheduled for pyloromyotomy according to the Weber–Ramstedt technique were retrospectively analysed. After sedation with nalbuphine and propofol, an ultrasound guided single shot thoracic epidural anaesthesia was performed with 0.75 ml·kg−1 ropivacaine 0.475%. Insufficient blockade was defined as increase of HR > 15% from initial value and/or any movements at skin incision. In those cases we were prepared for rapid sequence intubation according to the departmental standard.Results:  All pyloromyotomies could be performed under single shot thoracic epidural anaesthesia and sedation. One case of moderate oxygen desaturation was treated with intermittent ventilation via face mask.Conclusions:  Thoracic epidural anaesthesia under sedation for pyloromyotomy has been a useful technique in this retrospective series of infants suffering from HPS. In 1/20 infants short term assisted ventilation via face mask was required. Undisturbed surgery was possible in all cases.
Comparison of effective inspired concentration of sevoflurane in preterm infants with different postconceptual ages
Authors: Yu Ling , Sun Hongwei , Yao Lan , Feng Yi , Yang Baxian 
Published: November 2010

SummaryObjective:  Screening examination for retinopathy of prematurity (ROP) is stressful and painful to the neonate. Sevoflurane has been used successfully in anesthesia for full‐term and premature neonates and has been recently used for pediatric outpatient procedures. In this study, we examined the effective inspired concentration of sevoflurane to prevent movement in response to stimulation of examination in 50% of patients (EIC50) of sevoflurane, as well as the response of sevoflurane to age in the preterm outpatients undergoing fundus examination.Methods:  Preterm pediatric outpatients at different postconceptual ages (duration from the mother’s last menstrual period to the date when the examination for ROP was performed) with <44 weeks (group A) and 44–64 weeks (group B) undergoing fundus examination were included. SpO2 and ECG were monitored in operation room. In the process of anesthetic induction, the oxygen flow rates were 3 l·min−1, and subjects spontaneously breathed 6% sevoflurane by mask. The time to loss of movement was recorded as induction time. Six percent sevoflurane was inhaled continuously for the same duration, and then the inspired concentration of sevoflurane was adjusted to maintenance concentration. When no movement or crying was observed, the speculums were used to keep the eyelids open, and then the eye examinations were performed by the same ophthalmologist. During induction time and maintenance time, the occurrence of coughing, clenching, gross purposeful movement, breath holding or desaturation to SpO2 < 95% was recorded. After completion of the procedure, patients were observed in the same room until spontaneous eye opening or verbalization occurred. Up and down method was used to determine subsequent maintenance concentration in each group. The initial maintenance concentration was 3%. The gradient of increase or decrease was 0.5%. If the preceding subject had not moved, the sevoflurane concentration was decreased by 0.5%; if the preceding subject had moved, the concentration was increased by 0.5%. When at least six independent alternations from no‐movement to movement were observed, test was terminated.Results:  Twenty‐four pediatric outpatients completed the investigation in group A. The effective inspired concentration prevented movement in response to stimulation of examination in 50% of patients (EIC50) of sevoflurane was 2.5% in group A. Twenty‐seven patients completed the investigation and EIC50 values of sevoflurane were 3.0% in group B. The induction time in group A was significantly lower than that in group B. The gestational ages, anesthesia time, and awake time were similar in two groups.Conclusion:  Anesthesia with inhaled sevoflurane by a face mask can be accomplished in preterm outpatients undergoing fundus examination without intubation and i.v. accession. The EIC50 is lower, and the induction time is shorter in smaller aged patients compared with those in older ones.
Functional landscape heterogeneity and animal biodiversity in agricultural landscapes
Authors: Fahrig Lenore , Baudry Jacques , Brotons Lluís , Burel Françoise G. , Crist Thomas O. , Fuller Robert J. , Sirami Clelia , Siriwardena Gavin M. , Martin Jean‐Louis 
Published: November 2010

Ecology Letters (2011) 14: 101–112AbstractBiodiversity in agricultural landscapes can be increased with conversion of some production lands into ‘more‐natural’– unmanaged or extensively managed – lands. However, it remains unknown to what extent biodiversity can be enhanced by altering landscape pattern without reducing agricultural production. We propose a framework for this problem, considering separately compositional heterogeneity (the number and proportions of different cover types) and configurational heterogeneity (the spatial arrangement of cover types). Cover type classification and mapping is based on species requirements, such as feeding and nesting, resulting in measures of ‘functional landscape heterogeneity’. We then identify three important questions: does biodiversity increase with (1) increasing heterogeneity of the more‐natural areas, (2) increasing compositional heterogeneity of production cover types and (3) increasing configurational heterogeneity of production cover types? We discuss approaches for addressing these questions. Such studies should have high priority because biodiversity protection globally depends increasingly on maintaining biodiversity in human‐dominated landscapes.
The PprA–PprB two‐component system activates CupE, the first non‐archetypal Pseudomonas aeruginosa chaperone–usher pathway system assembling fimbriae
Authors: Giraud Caroline , Bernard Christophe S. , Calderon Virginie , Yang Liang , Filloux Alain , Molin Søren , Fichant Gwennaele , Bordi Christophe , de Bentzmann Sophie 
Published: November 2010

SummaryThe opportunistic pathogen Pseudomonas aeruginosa has redundant molecular systems that contribute to its pathogenicity. Those assembling fimbrial structures promote complex organized community lifestyle. We characterized a new 5.8 kb genetic locus, cupE, that includes the conserved usher‐ and chaperone‐encoding genes. This locus, widely conserved in different bacterial species, contains four additional genes encoding non‐archetypal fimbrial subunits. We first evidenced that the cupE gene cluster was specifically expressed in biofilm conditions and was responsible for fibre assembly containing at least CupE1 protein, at the bacterial cell surface. These fimbriae not only played a significant role in the early stages (microcolony and macrocolony formation) but also in shaping 3D mushrooms during P. aeruginosa biofilm development. Using wide‐genome transposon mutagenesis, we identified the PprAB two‐component system (TCS) as a regulator of cupE expression, and further demonstrated the involvement of the PprAB TCS in direct CupE fimbrial assembly activation. Thus, this TCS represents a new regulatory element controlling the transition between planktonic and community lifestyles in P. aeruginosa.