bluuebunny/crossref_metadata_2025_split huggingface dataset:
Protein glycosylation in sugar beet cell line can be influenced by DNA hyper- and hypomethylating agents
Authors: Pavoković Dubravko , Krsnik-Rasol Marijana 
Published: February 2012

Protein glycosylation in sugar beet cell line can be influenced by DNA hyper- and hypomethylating agentsProtein glycosylation is a co- and post-translational modification that influences protein function, stability and localization. Changes in glycoprotein pattern during differentiation/dedifferentiation events exist in animal cells and DNA methylation status is closely related to the changes. However, in plant cells this relationship is not yet established. In order to verify whether such a relation exists, hypermethylating drugs 2,4-dichlorophenoxyacetic acid and hydroxyurea, or hypomethylating drug 5-azacytozine were applied to sugar beet (Beta vulgarisL.) cells during 14 days of in vitro subculture, and the glycoprotein patterns of the cells were compared. The applied drugs were not toxic, as observed from cell phenotype and by measuring growth of the control and treated cells. Hyper and hypomethylating treatments influenced the activity of enzymes related to differentiation state of the cells: peroxidases and esterases, and their isoform patterns. Electrophoretic patterns of soluble and membrane proteins were similar between control and treatments, but the treatments modified N- and O-linked glycoprotein patterns as visible from GNA and PNA lectin blots. This suggested that hypermethylation and hypomethylation of genomic DNA in sugar beet cells affect protein glycosylation patterns and cellular metabolism, possibly in a mechanism similar to that existing in animal cells.
Fungal associations in gametophytes and young sporophytic roots of the fern Nephrolepis exaltata
Authors: Muthukumar Thangavelu , Prabha Kandasamy 
Published: February 2012

Fungal associations in gametophytes and young sporophytic roots of the fernNephrolepis exaltataInformation is limited on the presence of endophytic fungal associations in green gametophytes and young sporophytes of extant ferns. Nothing is known about their presence in Polypodiales, the largest order among extant ferns. We screened chlorophyllous gametophytes and young sporophytes ofNephrolepis exaltata(L.) Schott., (Lomariopsidaceae, Polypodiales) growing naturally on soil, brick and coir for the presence of fungal endophytes. Gametophytes and young sporophytes growing on different substrates were invariably colonized by septate endophytic fungi. Hyaline or brown, regularly septate, inter- or intracellular hyphae with moniliform cells or microsclerotia characterized septate endophytic fungi. However, only the roots of young sporophytes growing on soil and bricks harboured arbuscular mycorrhizal (AM) fungi. The AM morphology conformed to the intermediate type with intracellular hyphal coils, arbusculate coils and intercellular hyphae. No AM fungal spores could be retrieved from the soil on which gametophytes and young sporophytes were growing. The observations in this study support the idea that the septate fungal endophytes could confer an ecological advantage on colonized individuals, especially on nutrient deficient substrates.
Effect of supplemental Ca2+ on NaCl-stressed castor plants (Ricinus communis L.)
Authors: Joshi Seema , Patel Neha , Pandey Indu , Pandey Amar 
Published: February 2012

Effect of supplemental Ca2+on NaCl-stressed castor plants (Ricinus communisL.)Greenhouse experiments were conducted to assess the effects of supplemental Ca2+in salinised soil on germination and plant growth response of castor plant (Ricinus communisL. Var. Avani-31, Euphorbiaceae). NaCl amounting to 390 g was thoroughly mixed with soil of seven lots, of 100 kg each, to give electrical conductivity of 4.1 dS m-1. Further, Ca(NO3)2x 4H20 to the quantity of 97.5, 195, 292.5, 390, 487.5, and 585 g was separately mixed with soil of six lots to give 1:0.25, 1:0.50, 1:0.75, 1:1, 1:1.25, and 1:1.50 Na+/Ca2+ratios, respectively. The soil of the seventh lot contained only NaCl and its Na+/Ca2+ratio was 1:0. Soil without addition of NaCl and Ca (NO3)2x 4H20 served as control, with a 0:0 Na+/Ca2+ratio. Salinity significantly retarded seed germination and plant growth, but the deleterious effects of NaCl on seed germination were ameliorated and plant growth was restored with Ca2+supply at the critical level (1:0.25 Na+/Ca2+ratio) to salinised soil. Supply of Ca2+above the critical level further retarded seed germination and plant growth due to the increased soil salinity. Salt stress reduced N, P, K+and Ca2+content in plant tissues, but these nutrients were restored by addition of Ca2+at the critical level to saline soil. In contrast, Na+content in plant tissues significantly increased in response to salinity, but significantly decreased with increasing Ca2+supply to saline soil. The results are discussed in terms of the beneficial effects of Ca2+supply on the plant growth ofRicinus communisgrown under saline conditions.
Optimizing Motion Planning for Hyper Dynamic Manipulator
Authors: Aboura Souhila , Omari Abdelhafid , Meguenni Kadda 
Published: February 2012

Optimizing Motion Planning for Hyper Dynamic ManipulatorThis paper investigates the optimal motion planning for an hyper dynamic manipulator. As case study, we consider a golf swing robot which is consisting with two actuated joint and a mechanical stoppers. Genetic Algorithm (GA) technique is proposed to solve the optimal golf swing motion which is generated by Fourier series approximation. The objective function for GA approach is to minimizing the intermediate and final state, minimizing the robot's energy consummation and maximizing the robot's speed. Obtained simulation results show the effectiveness of the proposed scheme.
Effects of a Proximal or Distal Tibiofibular Joint Manipulation on Ankle Range of Motion and Functional Outcomes in Individuals With Chronic Ankle Instability
Authors: Beazell James R. , Grindstaff Terry L. , Sauer Lindsay D. , Magrum Eric M. , Ingersoll Christopher D. , Hertel Jay 
Published: February 2012

Study Design Randomized clinical trial. Objectives To determine whether manipulation of the proximal or distal tibiofibular joint would change ankle dorsiflexion range of motion and functional outcomes over a 3-week period in individuals with chronic ankle instability. Background Altered joint arthrokinematics may play a role in chronic ankle instability dysfunction. Joint mobilization or manipulation may offer the ability to restore normal joint arthrokinematics and improve function. Methods Forty-three participants (mean ± SD age, 25.6 ± 7.6 years; height, 174.3 ± 10.2 cm; mass, 74.6 ± 16.7 kg) with chronic ankle instability were randomized to proximal tibiofibular joint manipulation, distal tibiofibular joint manipulation, or a control group. Outcome measures included ankle dorsiflexion range of motion, the single-limb stance on foam component of the Balance Error Scoring System, the step-down test, and the Foot and Ankle Ability Measure sports subscale. Measurements were obtained prior to the intervention (before day 1) and following the intervention (on days 1, 7, 14, and 21). Results There was no significant change in dorsiflexion between groups across time. When groups were pooled, there was a significant increase (P<.001) in dorsiflexion at each postintervention time interval. No differences were found among the Balance Error Scoring System foam, step-down test, and Foot and Ankle Ability Measure sports subscale scores. Conclusions The use of a proximal or distal tibiofibular joint manipulation in isolation did not enhance outcome effects beyond those of the control group. Collectively, all groups demonstrated increases in ankle dorsiflexion range of motion over the 3-week intervention period. These increases might have been due to practice effects associated with repeated testing. Level of Evidence Therapy, level 2b-. J Orthop Sports Phys Ther 2012;42(2):125–134. doi:10.2519/jospt.2012.3729
Knowledge of and attitudes to Occupational Health & Safety among tutors of a Vocational Training Institute (IIEK) in Greece: A pilot study.
Authors: Bibila Stavroula 
Published: February 2012

This study aimed to develop two instruments, one for measuring knowledge of Occupational Health & Safety (OHS) and one for measuring attitudes to OHS, to examine differences in knowledge and attitudes among tutors teaching at a private vocational training institute in Greece (IIEK) and to identify significant predictors of OHS knowledge.For the 9-item knowledge scale developed, a K–R 20 of 0.60 was generated while for the 9-item attitude scale a Cronbach’s alpha (α) of 0.71 was generated. A cross-sectional, comparative research design was followed and the population (N=71) was stratified into “vocational area of expertise” groups. A proportional stratified random sampling strategy was used. It was found that, for the sampled tutors (n=31), “hours of OHS training” was the sole significant predictor of OHS knowledge contributing for 76% of the explained variance. No significant contributions to OHS knowledge were made by “vocational area of expertise”, “years of teaching experience” and “attitudes to OHS”. DOI: http://dx.doi.org/10.3126/ijosh.v2i1.5341 International Journal of Occupational Safety and Health, Vol. 2 No. 1 (2012) 15-25
YouTube as a Source of Information on Confined Space Safety
Authors: Brad Muise A , Freeman Brad , Blair Earl 
Published: February 2012

Safety professionals looking for information on confined space safety often use the Internet as a resource. YouTube is a popular website that may be used to supplement safety training or as a source of information pertaining to Confined Spaces (CS). YouTube was examined as a source of information on CS safety. YouTube was queried using key phrases “confined space,” “confined space entry,” and “confined space rescue.” Two safety experts reviewed each video and assigned scores for accuracy and view-ability. Of the 220 videos screened, 48 were found to have relevant information about CS safety and were selected for inclusion in the study. Approximately 70.8% of the videos were rated as inaccurate and 87.5% were rated as offering little value. Results of our study suggest that YouTube may currently be an inadequate source of information on CS safety. Safety professionals should verify YouTube video content against trusted agencies such as OSHA before using them as a resource for CS information.DOI: http://dx.doi.org/10.3126/ijosh.v2i1.5626International Journal of Occupational Safety and Health, Vol. 2 No. 1 (2012) 39-42
Differential pollinator effectiveness and importance in a milkweed (Asclepias, Apocynaceae) hybrid zone
Authors: Stoepler Teresa M. , Edge Andrea , Steel Anna , O'Quinn Robin L. , Fishbein Mark 
Published: February 2012

•Premise of the study:Exceptions to the ideal of complete reproductive isolation between species are commonly encountered in diverse plant, animal, and fungal groups, but often the causative ecological processes are poorly understood. In flowering plants, the outcome of hybridization depends in part on the effectiveness of pollinators in interspecific pollen transport. In theAsclepias exaltataandA. syriaca(Apocynaceae) hybrid zone in Shenandoah National Park, Virginia, extensive introgression has been documented. The objectives of this study were to (1) determine the extent of pollinator overlap amongA. exaltata,A. syriaca, and their hybrids and (2) identify the insect taxa responsible for hybridization and introgression.•Methods:We observed focal plants of parental species and hybrids to measure visitation rate, visit duration, and per‐visit pollinia removal and deposition, and we calculated pollinator effectiveness and importance.•Key results:Visitation rates varied significantly between the 2 yr of the study. Overall,Apis mellifera,Bombussp., andEpargyreus claruswere the most important pollinators. However,Bombussp. was the only visitor that was observed to both remove and insert pollinia for both parent species as well as hybrids.•Conclusions:We conclude thatBombusmay be a key agent of hybridization and introgression in these sympatric milkweed populations, and hybrids are neither preferred nor selected against by pollinators. Thus, we have identified a potential mechanism for how hybrids act as bridges to gene flow betweenA. exaltataandA. syriaca. These results provide insights into the breakdown of prezygotic isolating mechanisms.
Microsatellite markers for the relictual dove tree, Davidia involucrata (Cornaceae)
Authors: Tao Chengcheng , Yang Zhenyan , Lu Ranran , Liu Tao , Chen Shaotian , Ji Yunheng 
Published: February 2012

• Premise of the study: Microsatellite markers were developed for the dove tree, Davidia involucrata (Cornaceae), a Tertiary relict currently endemic to China, to investigate its population genetics and phylogeography.• Methods and Results: Using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol, nine polymorphic microsatellite loci were identified and screened in 44 individuals from three wild populations of D. involucrata. The number of alleles per locus ranged from two to three, while the observed heterozygosity and expected heterozygosity ranged from 0.0000 to 0.6000 and from 0.0000 to 0.6323, respectively.• Conclusions: These new microsatellite loci will facilitate further studies of the population genetics and phylogeography of D. involucrata, as well as of the evolutionary history of the plant and other Tertiary relicts endemic to East Asia.
Microsatellite loci for Gossypium davidsonii (Malvaceae) and other D‐genome, Sonoran Desert endemic cotton species
Authors: Kuester Adam P. , Nason John D. 
Published: February 2012

• Premise of the study: Microsatellite primers previously developed for domesticated cotton (Gossypium hirsutum; tetraploid) were screened for their utility in investigating genetic structure and gene flow within G. davidsonii and five other wild, Mexican, D‐genome cotton species (all diploid).• Methods and Results: We screened 50 microsatellite primer pairs from the Cotton Marker Database, identifying 10 loci as polymorphic within G. davidsonii. In genotyping approximately 200 individuals from four populations, we found that the number of alleles per locus ranged from seven to 17, and mean observed and expected heterozygosity ranged from 0.145 to 0.492 and from 0.436 to 0.734, respectively. We genotyped six to 20 individuals from each of the remaining species, finding these 10 loci to cross‐amplify in all cases and in most cases to be polymorphic.• Conclusions: These markers may be useful for further investigation of population genetics of G. davidsonii and other wild D‐genome cotton species.
Development of microsatellite markers in Byrsonima crassifolia (Malpighiaceae)
Authors: Croft Genevieve K. , Schaal Barbara A. 
Published: February 2012

• Premise of the study: We developed and characterized microsatellite markers for Byrsonima crassifolia (Malpighiaceae), a widely distributed neotropical fruit tree.• Methods and Results: Eight polymorphic and two monomorphic microsatellite loci were identified and screened in 60 samples from four geographically disparate populations (Bolivia, Brazil, Mexico, and Panama). Each locus exhibited between two and 11 alleles. Expected heterozygosity ranged from 0 to 0.839. All loci amplify in the congeners B. variabilis and B. basiloba, four amplify in B. bucidaefolia, and seven amplify in B. variabilis, although levels of polymorphism have not been assessed.• Conclusions: These loci will provide novel tools for comparing genetic diversity present in cultivated and noncultivated populations of B. crassifolia throughout its range, and may prove valuable in related species.
Pill Burden in HIV Infection: 20 Years of Experience
Authors: Krentz Hartmut B , Cosman Ian , Lee Kathy , Ming Jinell Mah , Gill M John 
Published: February 2012

Background New antiretroviral (ARV) drugs and improved formulations and coformulations of existing ARVs are actively promoted to diminish a patient's pill burden and to minimise the opportunity for mismatched dosing, although the effect of these advances is poorly understood. We determine how these changes affect the total daily pill burden (TDPB) for ARV and other drug use over a 20-year period. Methods Using our in-house pharmacy database, we calculated the daily number and associated pill burden of oral ARV and non-ARV (prescription and over-the-counter) medications taken by every patient within the Southern Alberta Cohort (SAC) between 1990 and 2010. We compared the mean TDPB with the patients’ sociodemographic and clinical characteristics. Results Mean TDPB for ARV-experienced patients increased from 4.9 in 1990 to 12.1 in 1998 but decreased to 6.7 in 2010. By 2010, new ARVs and newer formulations had decreased ARV pill burden by 50% over five years. In 1990, however, 95% of the TDPB consisted of ARVs but by 2010 it fell to 51% as non-ARV daily drugs increased from 0.2 in 1990 to 2.6 in 2010. Variation in TDPB was attributable to four main factors: patient's age, risk factor, nadir CD4+ T-cell count and duration of known HIV infection. Conclusions While new ARV formulations and coformulations have simplified regimens, this reduction in ARV pill burden has been counterbalanced by increases in non-ARV drugs required for managing comorbidities. Discussions on merits of coformulations in decreasing ARV pill burden need to include the non-ARV pill burden.
La morale de l'urgence sociale. Une enquête au Samu social de Paris
Authors: Gardella Edouard , Cefaï Daniel 
Published: February 2012

Résumé L’assistance aux personnes dites « sans domicile fixe » s’est institutionnalisée depuis les années 1980 sous le mot d’ordre de l’« urgence sociale ». Cette politique publique, inspirée de l’action humanitaire, est souvent critiquée pour avoir mis en place une « politique de la pitié ». Une enquête ethnographique auprès des maraudes du Samu social de Paris montre la complexité des sentiments moraux qui animent ce type d’assistance à la rue : la compassion y prend un sens de sollicitude et de reconnaissance, dans l’horizon de l’accès aux droits.
Cette année encore, l'hiver reviendra au mois de décembre...
Authors: Tuleu Fabien 
Published: February 2012

Résumé Dans cet article, l’auteur analyse plus de vingt ans de pratiques professionnelles dans le champ de l’urgence sociale héritière d’un « transfert analogique » du secteur médical. Les questions abordées portent sur les fondements politiques de l’urgence, ses limites du point de vue clinique et la mise en perspective de deux scénarios pour les pratiques professionnelles. L’auteur avance l’hypothèse que l’urgence sociale traitée annuellement par des plans successifs voile les symptômes d’une crise plus profonde de la solidarité et du logement social.
État d'urgence pour l'urgence sociale
Authors: Garcia Bruno 
Published: February 2012

Résumé Le secteur de l’urgence sociale traverse actuellement une crise profonde, qui se traduit par une forte diminution des possibilités d’accueil et d’hébergement des personnes en détresse et sans abri. Les causes en sont multiples : augmentation des besoins et des demandes d’hébergement d’urgence, places d’hébergement qui restent occupées plus longtemps et mise en œuvre de la politique du « Logement d’abord » au détriment du secteur de l’urgence.
La fabrique de l'intravail
Authors: Castejon Christine 
Published: February 2012

Résumé On ne trouvera pas de dirigeant pour dire qu’il a fait de l’urgence permanente son mode de management. Or l’urgence est bel et bien un mode de management comme l’état d’urgence est un mode de gouvernement. Elle suspend tout ce qui n’est pas la stricte mobilisation autour d’un objectif ou d’une cible. Mais elle nous dépossède de l’arbitrage dans nos priorités, qui est à la fois un droit élémentaire et une condition de notre efficacité. Il n’y a pas de baguette magique pour sortir de la spirale : à chacun de faire barrage.
Le tonneau des Danaïdes... l'urgence nécessaire et sans fin
Authors: Roche Jean-François 
Published: February 2012

Résumé Les situations d’urgence comportementale sont au carrefour des impuissances des systèmes éducatifs, de protection de l’enfance et de soins ; ces situations sont des agents de destruction tant des psychismes individuels que des structures de prise en charge ; les réponses possibles ne sont pas dans l’urgence mais dans la durée, la continuité et l’authentique multidisciplinarité.
L'enfant dans les séparations conflictuelles : le rôle de la justice
Authors: Mulon Élodie 
Published: February 2012

Résumé L’enfant, lors de la séparation des parents, est au cœur d’un conflit dont il n’est pas responsable et qui n’est pas le sien. Pour autant, il en devient à la fois l’enjeu, l’instrument, l’acteur, la victime et le bourreau. Il est essentiel que le processus de séparation, judiciaire et extrajudiciaire, lui permette de rester à sa place d’enfant, étranger au conflit de ses parents, et ce quel que soit son âge. Cette nécessité commence par une prise de conscience impérative de la part des parents : la justice n’a ni l’envie ni les moyens de se substituer à eux.
L'obésité morbide chez l'adolescent : les réponses d'un réseau multidisciplinaire
Authors: Malka Jean , Duverger Philippe , Weil Dominique 
Published: February 2012

Résumé L’obésité morbide est ici abordée en tant que symptôme physique d’une souffrance psychique sous-jacente. Cette souffrance psychique génère parfois certaines demandes d’aide, notamment chirurgicale, qui paradoxalement révèlent bien souvent un très fort déni de la réalité de cette souffrance, engageant alors autant le jeune que les soignants dans une lutte active contre l’obésité plutôt qu’une lutte contre la souffrance psychique qui en est à l’origine. Cet article s’intéresse à la demande de chirurgie bariatrique de la part du jeune et de son environnement, demande souvent confondue avec la notion de « motivation », l’insistance de cette motivation n’ayant d’égal que l’ambivalence de la demande. Cette confusion de registre entre motivation affichée et demande ambivalente s’avère fréquemment à la source d’un malentendu qui met à l’épreuve autant l’offre que le cadre du soin. C’est à la lumière de ce constat qu’une approche multidisciplinaire intégrant les dimensions psychique et somatique impliquées dans l’obésité morbide est proposée au chu d’Angers.
Interaction entre racines unitaires et ruptures structurelles
Authors: Bassil Charbel 
Published: February 2012

Résumé Dans cet article, nous passons en revue certaines méthodes économétriques récentes liées aux tests de racines unitaires. L’idée centrale est l’interaction entre ruptures structurelles et racines unitaires. Nous nous intéressons au test standard Dickey-Fuller et à ces modifications qui permettent sous l’alternative une ou plusieurs ruptures. Les dates de ruptures sont endogènes et le nombre de ruptures peut être inconnu. Nous investiguons la taille et la puissance de ces tests. Nous considérons ainsi le problème d’estimation du nombre de ruptures structurelles et le problème d’estimation des dates de ruptures structurelles. Une deuxième catégorie de test est examinée, les tests lm de racine unitaire permettant, sous les deux hypothèses, une ou deux ruptures inconnues. Nous survolons aussi les tests de ruptures structurelles construits pour un univers stationnaire. Nous distinguons deux catégories de tests : les tests pour une seule rupture et les tests pour plusieurs ruptures.