bluuebunny/crossref_metadata_2025_split huggingface dataset:
ADAMTS‐13 activity can predict the outcome of disseminated intravascular coagulation in hematologic malignancies treated with recombinant human soluble thrombomodulin
Authors: Ohshiro Muneo , Kuroda Junya , Kobayashi Yutaka , Akaogi Teruaki , Kawata Eri , Uoshima Nobuhiko , Kamitsuji Yuri , Kaneko Hiroto , Shimura Kazuho , Shimazaki Chihiro , Murakami Satoshi , Hatsuse Mayumi , Okano Akira , Kobayashi Tsutomu , Uchiyama Hitoji , Matsumoto Yosuke , Horiike Shigeo , Taniwaki Masafumi 
Published: September 2011

AbstractWe conducted a multicenter prospective study for evaluating the utility and prognostic markers of recombinant human soluble thrombomodulin (rTM) treatment for acute disseminated intravascular coagulation (DIC) by various types of hematologic malignancies. The study comprised 30 patients with DIC due to hematologic diseases without severe infection. DIC improved in 15 patients and 20 were alive on day 28. Univariate analyses showed that, in comparison with patients who had survived on day 28, patients who had not survived on day 28 showed significantly higher plasma levels of plasminogen activator inhibitor‐I (PAI‐I) and significantly lower plasma activity of a disintegrin and metalloproteinase with a thrombospondin Type 1 motif, member 13 (ADAMTS‐13). Moreover, multivariate logistic regression analysis identified a significant association between plasma ADAMTS‐13 activity before treatment and survival on day 28 (P = 0.034). In particular, patients with lower ADAMTS‐13 activity (≤65%) had a poorer survival rate than those with a higher activity (P = 0.042). These findings suggest that the plasma ADAMTS‐13 activity at the time of DIC diagnosis might help to predict the prognosis of patients treated with rTM for DIC associated with hematologic malignancies. Am. J. Hematol., 2012. © 2011 Wiley‐Liss, Inc.
Peaceful primates: affiliation, aggression, and the question of female dominance in a nocturnal pair‐living lemur (Avahi occidentalis)
Authors: Ramanankirahina Rindrahatsarana , Joly Marine , Zimmermann Elke 
Published: September 2011

AbstractAffiliation/agonism and social dominance are central factors determining social organization in primates. The aim of our study is to investigate and describe, for the first time, the intersexual relations in a nocturnal and cohesive pair‐living prosimian primate, the western woolly lemur (Avahi occidentalis), and to determine to what extent phylogeny, activity mode, or the cohesiveness of pair partners shape the quality of social interactions. Six pairs of western woolly lemurs were radio‐collared in the dry deciduous forest of northwestern Madagascar. More than 874 hr of focal animal sampling were conducted. All occurrences of social interactions involving a focal animal were recorded. The rate of affiliation between pair partners was significantly higher than the rate of agonism. Western woolly lemur pairs' interactions were extremely peaceful. All decided agonistic conflicts (N = 15) were exclusively initiated and won by the female. No female showed spontaneous submission toward her male partner. These results are in line with those of diurnal cohesive pair‐living anthropoid primates. Findings support the hypothesis that social relations in pair‐living primates are linked to the cohesiveness of pair partners in time and space irrespective of phylogeny and activity mode. Am. J. Primatol. 73:1261–1268, 2011. © 2011 Wiley Periodicals, Inc.
Wirte für Fullerene: maßgeschneiderte Makrocyclen und Käfige
Authors: Canevet David , Pérez Emilio M. , Martín Nazario 
Published: September 2011

AbstractMaßgeschneiderte makrocyclische Fullerenrezeptoren erweisen sich als hochaffine Systeme für die selektive Extraktion höherer Fullerene und deren Racematspaltung sowie für die Selbstorganisation funktioneller molekularer Materialien. Dieser Kurzaufsatz fasst einige der wichtigsten Fortschritte zusammen, die auf dem Gebiet der Fullerenwirte erzielt wurden.
Homogeneous hydrogenation of electron‐deficient alkenes by iridium complexes
Authors: Semeniuchenko Volodymyr , Exner Thomas E. , Khilya Volodymyr , Groth Ulrich 
Published: September 2011

AbstractThe catalytic homogeneous hydrogenation of electron‐deficient alkenes (nucleophilic hydrogenation) was achieved in the presence of iridium complexes and a base as co‐catalyst. Contrary to hydrogenation of electron‐rich alkenes, which is inactivated by bases, the hydrogenation of the electron‐deficient alkenes turned out to be base activated. Here, we present a more thorough study on the capacities but also limitations of this new reaction mechanism using screenings of the reaction conditions as well as different Ir complexes and substrates. The formation of a catalytically active Ir complex is proposed. The active complex usually attacks a soft electron‐deficient atom, if more than one possibility exists (as shown by density functional theory computations). Additionally, first examples of enantiomeric enrichments in the presence of chiral Ir complexes are presented. The high catalyst load needed and the moderate yields show that the active complex is very unstable under conditions of nucleophilic hydrogenation and is quickly deactivated, which has to be addressed in further studies. Copyright © 2011 John Wiley & Sons, Ltd.
Preparation and characterization of carbon black‐polystyrene composite particles by high‐speed homogenization assisted suspension polymerization
Authors: Liu Hongbo , Wen Shaoguo , Wang Jihu , Zhu Yu 
Published: September 2011

AbstractComposite particles of carbon black‐polystyrene with controllable size were successfully prepared using high‐speed homogenization‐assisted suspension polymerization. The carbon black particles were modified by oleic acid to make it more hydrophobic and more compatible with polymers before the encapsulation process. The effects of homogenization speed, homogenization time, agitation speed, pigment concentration, and stabilizer concentration on the properties of complex spheres were investigated in detail. The results of orthogonal experiments proved that the stabilizer concentration plays the most important role in controlling the size of complex particles. The particles were also characterized by FTIR and SEM. SEM photographs proved that the complex particles had good spherical forms with smooth surfaces. © 2011 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Insights Into Coronary Artery Development in Model of Maternal Protein Restriction in Mice
Authors: Silva‐Junior Geraldo O. , Aguila Marcia B. , Mandarim‐de‐Lacerda Carlos A. 
Published: September 2011

AbstractProgramming of fetal development is considered to be an important risk factor for noncommunicable diseases of adulthood, including coronary heart disease (CHD). Aiming to investigate the association between maternal nutrition and the development of the coronary arteries (CAs) in staged mice embryos, C57BL/6 mice embryos from Stages 16 to 23 were taken from mothers fed a normal protein (NP) or low protein (LP) diet, and the CA were studied. Although the LP embryos had lower masses, they had faster heart growth rates when compared with the NP embryos. The subepicardial plexuses were observed earlier in the NP embryos (Stage 20) than in the LP ones (Stage 22; P < 0.01). Apoptotic nuclei were seen around the aortic peritruncal ring beginning at Stage 18 in the NP and LP embryos. FLK1+ (fetal liver kinase 1 = VEGF‐r2 or vascular endothelial growth factor receptor 2) cells had a homogeneous distribution in the NP embryos as early as Stage 18, whereas a similar distribution in the LP embryos was only seen at Stages 22 and 23. Maternal protein restriction in mice leads to a delay in the growth of the heart in the embryonic period modifying the development of the subepicardial peritruncal plexus and the apoptosis in the future coronary orifice region. Anat Rec, 2011. © 2011 Wiley‐Liss, Inc.
Distribution and Ultrastructure of Afferent Fibers in the Parietal Peritoneum of the Rat
Authors: Tanaka Koichi , Hayakawa Tetsu , Maeda Seishi , Kuwahara‐Otani Sachi , Seki Makoto 
Published: September 2011

AbstractThe distribution and the ultrastructure of afferent fibers innervating the parietal peritoneum in the rat was studied with immunohistochemistry using an antiserum against the neuronal marker protein gene product 9.5. The immunoreactive fibers were distributed throughout the peritoneum. They generally ran straight and parallel to the intercostal nerves running in the abdominal muscles underlying the peritoneum. They sometimes branched and terminated by forming club‐like endings. The number of nerve endings on the peritoneal surface was 3.25 ± 1.66 mm−2. Electron microscopic observations revealed both unmyelinated and myelinated nerve fibers. The unmyelinated fibers were thin and about 1 μm in diameter. Their endings formed slight swellings located just inside the peritoneal cell layer. The myelinated fibers often formed a bundle that was composed of two or three nerve fibers. Each myelinated fiber kept in contact with a Shwann cell and projected toward the peritoneal cavity. Finally, they penetrated the peritoneal cell layer to reach the peritoneal cavity. These fibers then made contacts with the peritoneal cells and became free from the myelin sheath. The ending had a club‐like shape covered with collagen fibers, and contained many neurofilaments, a few mitochondria, but no synaptic vesicles. These results suggest that since the sensory endings are exposed at the peritoneal cavity, the sensory fibers are highly sensitive to somatic or nociceptive stimuli. Anat Rec, 2011. © 2011 Wiley‐Liss, Inc.
A Candidate Circuit Approach to Investigating Autism
Authors: Page Damon T. 
Published: September 2011

AbstractA major problem in understanding mechanisms of pathogenesis in autism spectrum disorder (ASD) is deciphering how risk factors act via the brain to influence the behavioral symptoms of this disorder. We may start to bridge this gap in our understanding by systematically examining the structure and function of cell types that make up circuits underlying behavioral endophenotypes in animal models for ASD. A confluence of advances in basic behavioral neurobiology, in ASD mechanisms and animal models, and in genetic tools for imaging and manipulating brain circuits will make this possible. Through a process of elimination of candidates and comparison across models, we may hope to understand how ASD risk factors influence the development and function of neural circuitry at the level of genetically defined cell types. As an example of how this candidate circuit approach may be applied to investigating ASD, here I focus on social behavior as an endophenotype, and I discuss recent findings regarding the development and function of the oxytocin system, which is implicated in both normal social behavior and ASD pathogenesis. I stress the importance of a collaborative, multidisciplinary approach to probing candidate cell types and circuits across mouse models of ASD. Anat Rec,, 2011. © 2011 Wiley‐Liss, Inc.
Lack of a chondroprotective effect of cyclooxygenase 2 inhibition in a surgically induced model of osteoarthritis in mice
Authors: Fukai Atsushi , Kamekura Satoru , Chikazu Daichi , Nakagawa Takumi , Hirata Makoto , Saito Taku , Hosaka Yoko , Ikeda Toshiyuki , Nakamura Kozo , Chung Ung‐il , Kawaguchi Hiroshi 
Published: September 2011

AbstractObjectiveTo investigate the chondroprotective effect of cyclooxygenase 2 (COX‐2) inhibition in experimental osteoarthritis (OA).MethodsThe expression of prostaglandin E2synthetic enzymes was examined by immunostaining of tibial cartilage from mice with surgically induced knee joint instability and from OA patients undergoing total knee arthroplasty. The effect of orally administered celecoxib (10 mg/kg/day and 30 mg/kg/day) or vehicle alone in mice was examined 12 weeks after the induction of OA. To investigate the involvement of COX‐1 and COX‐2 in OA development, we also created the model in COX‐1–homozygous‐knockout (Ptgs1−/−) mice and COX‐2–homozygous‐knockout (Ptgs2−/−) mice. OA severity was assessed using a grading system developed by our group and by the Osteoarthritis Research Society International scoring system.ResultsIn mouse and human OA cartilage, the expression of the inducible enzymes COX‐2 and microsomal prostaglandin E synthase 1 (mPGES‐1) was enhanced, while that of the constitutive enzymes COX‐1, cytosolic PGES, and mPGES‐2 was suppressed. Daily celecoxib treatment did not prevent cartilage degradation or osteophyte formation during OA development in the mouse model. Furthermore, neitherPtgs1−/−mice norPtgs2−/−mice exhibited any significant difference in OA development as compared to wild‐type littermates.ConclusionThe two COX enzymes differ in terms of regulation of their expression during OA development. Nevertheless, experiments using inhibitor and genetic deficiency demonstrated a lack of chondroprotective effect of COX‐2 inhibition in the mouse surgical OA model.
A rare polymorphism in the gene for Toll‐like receptor 2 is associated with systemic sclerosis phenotype and increases the production of inflammatory mediators
Authors: Broen J. C. A. , Bossini‐Castillo L. , van Bon L. , Vonk M. C. , Knaapen H. , Beretta L. , Rueda B. , Hesselstrand R. , Herrick A. , Worthington J. , Hunzelman N. , Denton C. P. , Fonseca C. , Riemekasten G. , Kiener H. P. , Scorza R. , Simeón C. P. , Ortego‐Centeno N. ,   Spanish Systemic Sclerosis Group, Gonzalez‐Gay M. A. , Airò P. , Coenen M. J. H. , Martín J. , Radstake T. R. D. J. 
Published: September 2011

AbstractObjectiveTo investigate whether polymorphisms in Toll‐like receptor (TLR) genes, previously reported to be associated with immune‐mediated diseases, are involved in systemic sclerosis (SSc).MethodsWe genotyped 14 polymorphisms in the genes for TLRs 2, 4, 7, 8, and 9 in a discovery cohort comprising 452 SSc patients and 537 controls and a replication cohort consisting of 1,170 SSc patients and 925 controls. In addition, we analyzed 15‐year followup data on 964 patients to assess the potential association of TLR variants with the development of disease complications. We analyzed the functional impact of the associated polymorphism on monocyte‐derived dendritic cells.ResultsIn the discovery cohort, we observed that a rare functional polymorphism in TLR2 (Pro631His) was associated with antitopoisomerase (antitopo) positivity (odds ratio 2.24 [95% confidence interval 1.24–4.04], P = 0.003). This observation was validated in the replication cohort (odds ratio 2.73 [95% confidence interval 1.85–4.04], P = 0.0001). In addition, in the replication cohort the TLR2 variant was associated with the diffuse subtype of the disease (P = 0.02) and with the development of pulmonary arterial hypertension (PAH) (Cox proportional hazards ratio 5.61 [95% confidence interval 1.53–20.58], P = 0.003 by log rank test). Functional analysis revealed that monocyte‐derived dendritic cells carrying the Pro63His variant produced increased levels of inflammatory mediators (tumor necrosis factor α and interleukin‐6) upon TLR‐2–mediated stimulation (both P < 0.0001).ConclusionAmong patients with SSc, the rare TLR2 Pro631His variant is robustly associated with antitopoisomerase positivity, the diffuse form of the disease, and the development of PAH. In addition, this variant influences TLR‐2–mediated cell responses. Further research is needed to elucidate the precise role of TLR‐2 in the pathogenesis of SSc.
Defective DNA double‐strand break repair in pediatric systemic lupus erythematosus
Authors: Davies Robert C. , Pettijohn Kelly , Fike Francesca , Wang Jiexi , Nahas Shareef A. , Tunuguntla Rashmi , Hu Hailiang , Gatti Richard A. , McCurdy Deborah 
Published: September 2011

AbstractObjectivePrevious reports of cells from patients with systemic lupus erythematosus (SLE) note that repair of single‐strand breaks is delayed, and these lesions may be converted to double‐strand breaks (DSBs) at DNA replication forks. We undertook this study to assess the integrity of DSB recognition, signaling, and repair mechanisms in B lymphoblastoid cell lines derived from patients with pediatric SLE.MethodsNine assays were used to interrogate DSB repair and recognition in lymphoblastoid cell lines from patients with pediatric SLE, including the neutral comet assay (NCA), colony survival assay (CSA), irradiation‐induced foci formation for γ‐H2AX and 53BP1 proteins, kinetics of phosphorylation of structural maintenance of chromosomes protein 1 (SMC1), postirradiation bromodeoxyuridine incorporation to evaluate S phase checkpoint integrity, monoubiquitination of Fanconi protein D2, ATM protein expression, and non‐homologous DNA end joining protein expression and function.ResultsThree of the 9 assays revealed abnormal patterns of response to irradiation‐induced DNA damage. The NCA and CSA yielded aberrant results in the majority of SLE lymphoblastoid cell lines. Abnormal prolongation of SMC1 phosphorylation was also noted in 2 of 16 SLE lymphoblastoid cell lines.ConclusionOur data suggest that DSB repair is defective in some lymphoblastoid cell lines from pediatric patients with SLE, especially when assessed by both NCA and CSA. Since these studies are nonspecific, further studies of DNA repair and kinetics are indicated to further delineate the underlying pathogenesis of SLE and possibly identify therapeutic targets.
Large candidate gene association study reveals genetic risk factors and therapeutic targets for fibromyalgia
Authors: Smith Shad B. , Maixner Dylan W. , Fillingim Roger B. , Slade Gary , Gracely Richard H. , Ambrose Kirsten , Zaykin Dmitri V. , Hyde Craig , John Sally , Tan Keith , Maixner William , Diatchenko Luda 
Published: September 2011

AbstractObjectiveFibromyalgia (FM) represents a complex disorder that is characterized by widespread pain and tenderness and is frequently accompanied by additional somatic and cognitive/affective symptoms. Genetic risk factors are known to contribute to the etiology of the syndrome. The aim of this study was to examine >350 genes for association with FM, using a large‐scale candidate gene approach.MethodsThe study group comprised 496 patients with FM (cases) and 348 individuals with no chronic pain (controls). Genotyping was performed using a dedicated gene array chip, the Pain Research Panel, which assays variants characterizing >350 genes known to be involved in the biologic pathways relevant to nociception, inflammation, and mood. Association testing was performed using logistic regression.ResultsSignificant differences in allele frequencies between cases and controls were observed for 3 genes: GABRB3 (rs4906902; P = 3.65 × 10−6), TAAR1 (rs8192619; P = 1.11 × 10−5), and GBP1 (rs7911; P = 1.06 × 10−4). These 3 genes and 7 other genes with suggestive evidence for association were examined in a second, independent cohort of patients with FM and control subjects who were genotyped using the Perlegen 600K platform. Evidence of association in the replication cohort was observed for TAAR1, RGS4, CNR1, and GRIA4.ConclusionVariation in these 4 replicated genes may serve as a basis for development of new diagnostic approaches, and the products of these genes may contribute to the pathophysiology of FM and represent potential targets for therapeutic action.
Sulfated Ligands for the Copper(I)‐Catalyzed Azide–Alkyne Cycloaddition
Authors: Wang Wei , Hong Senglian , Tran Andrew , Jiang Hao , Triano Rebecca , Liu Yi , Chen Xing , Wu Peng 
Published: September 2011

AbstractThe copper(I)‐catalyzed azide–alkyne cycloaddition (CuAAC), the prototypical reaction of click chemistry, is accelerated by tris(triazolylmethyl)amine‐based ligands. Herein, we compare two new ligands in this family—3‐[4‐({bis[(1‐tert‐butyl‐1H‐1,2,3‐triazol‐4‐yl)methyl]amino}methyl)‐1H‐1,2,3‐triazol‐1‐yl]propanol (BTTP) and the corresponding sulfated ligand 3‐[4‐({bis[(1‐tert‐butyl‐1H‐1,2,3‐triazol‐4‐yl)methyl]amino}methyl)‐1H‐1,2,3‐triazol‐1‐yl]propyl hydrogen sulfate (BTTPS)—for three bioconjugation applications: 1) labeling of alkyne‐tagged glycoproteins in crude cell lysates, 2) labeling of alkyne‐ or azide‐tagged glycoproteins on the surface of live mammalian cells, and 3) labeling of azides in surface proteins of live Escherichia coli. Although BTTPS exhibits faster kinetics than BTTP in accelerating the CuAAC reaction in in vitro kinetic measurements, its labeling efficiency is slightly lower than BTTP in modifying biomolecules with a significant amount of negative charges due to electrostatic repulsion. Nevertheless, the negative charge conferred by the sulfate at physiological conditions significantly reduced the cellular internalization of the coordinated copper(I), thus making BTTPS–CuI a better choice for live‐cell labeling.
Thermoresponsive Supramolecular Dendronized Polymers
Authors: Yan Jiatao , Li Wen , Liu Kun , Wu Dalin , Chen Feng , Wu Peiyi , Zhang Afang 
Published: September 2011

AbstractCombining the concepts of supramolecular polymers and dendronized polymers provides the opportunity to create bulky polymers with easy structural modification and tunable properties. In the present work, a novel class of side‐chain supramolecular dendronized polymethacrylates is prepared through the host–guest interaction. The host is a linear polymethacrylate (as the backbone) attached in each repeat unit with a β‐cyclodextrin (β‐CD) moiety, and the guest is constituted with three‐fold branched oligoethylene glycol (OEG)‐based first‐ (G1) and second‐generation (G2) dendrons with an adamantyl group core. The host and guest interaction in aqueous solution leads to the formation of the supramolecular polymers, which is supported with 1H NMR spectroscopy and dynamic light scattering measurements. The supramolecular formation was also examined at different host/guest ratios. The water solubility of hosts and guests increases upon supramolecular formation. The supramolecular polymers show good solubility in water at room temperature, but exhibit thermoresponsive behavior at elevated temperatures. Their thermoresponsiveness is thus investigated with UV/Vis and 1H NMR spectroscopy, and compared with their counterparts formed from individual β‐CD and the OEG dendritic guest. The effect of polymer concentration and molar ratio of host/guest was examined. It is found that the polar interior of the supramolecules contribute significantly to the thermally‐induced phase transitions for the G1 polymer, but this effect is negligible for the G2 polymer. Based on the temperature‐varied proton NMR spectra, it is found that the host–guest complex starts to decompose during the aggregation process upon heating to its dehydration temperature, and this decomposition is enhanced with an increase of solution temperature.
Detection of hydrogen sulphide using cataluminescence sensors based on alkaline‐earth metal salts
Authors: Liu Yonghui , Tang Fei , Kang Chol Jin , Cao Xiaoan 
Published: September 2011

ABSTRACTDetection of hydrogen sulphide (H2S) was conducted based on cataluminescence (CTL) sensors, using alkaline‐earth metal carbonates as catalysts. Optimal working conditions, analytical characteristics and the response properties of the sensor were investigated. CTL intensity examination showed that sensors fabricated with CaCO3, SrCO3 or BaCO3 could be used to detect H2S gas sensitively. The optimal sensing temperature was about 320 °C. Under the sensing conditions with temperature at ca. 320 °C and gas flow rate in the range 180–200 mL/min, the linear range of CTL intensity vs H2S concentration was 25–500 ppm, with a detection limit of 2 ppm. The response and recovery times of the sensor were within 5 and 25 min, respectively. Also, the sensor had the property of high selectivity to H2S with very weak or no obvious response to 14 other gases, such as NO2, NH3, hydrocarbons and alcohol. Copyright © 2011 John Wiley & Sons, Ltd.