bluuebunny/crossref_metadata_2025_split huggingface dataset:
Aerobic endurance training versus relaxation training in patients with migraine (ARMIG): study protocol for a randomized controlled trial
Authors: Totzeck Andreas , Unverzagt Susanne , Bak Maja , Augst Pierre , Diener Hans-Christoph , Gaul Charly 
Published: April 2012

Abstract

Background
Migraine is one of the most frequent headache diseases and impairs patients’ quality of life. Up to now, many randomized studies reported efficacy of prophylactic therapy with medications such as beta-blockers or anti-epileptic drugs. Non-medical treatment, like aerobic endurance training, is considered to be an encouraging alternative in migraine prophylaxis. However, there is still a lack of prospective, high-quality randomized trials. We therefore designed a randomized controlled trial to evaluate the efficacy of aerobic endurance training versus relaxation training in patients with migraine (ARMIG).

Methods
This is a single-center, open-label, prospective, randomized trial. Sixty participants with migraine are randomly allocated to either endurance training or a relaxation group. After baseline headache diary documentation over at least 4 weeks, participants in the exercise group will start moderate aerobic endurance training under a sport therapist’s supervision at least 3 times a week over a 12-week period. The second group will perform Jacobson’s progressive muscle relaxation training guided by a trained relaxation therapist, also at least 3 times a week over a 12-week period. Both study arms will train in groups of up to 10 participants. More frequent individual training is possible. The follow-up period will be 12 weeks after the training period. The general state of health, possible state of anxiety or depression, impairments due to the headache disorder, pain-related disabilities, the headache-specific locus of control, and the motor fitness status are measured with standardized questionnaires.

Discussion
The study design is adequate to generate meaningful results. The trial will be helpful in gaining important data on exercise training for non-medical migraine prophylaxis.

Trial registration
The trial is registered at ClinicalTrials.gov: NCT01407861.
Three dimensional socket preservation: a technique for soft tissue augmentation along with socket grafting
Authors: Gholami Gholam Ali , Aghaloo Maryam , Ghanavati Farzin , Amid Reza , Kadkhodazadeh Mahdi 
Published: April 2012

Abstract

Background
A cursory review of the current socket preservation literatures well depicts the necessity of further esthetic considerations through the corrective procedures of the alveolar ridge upon and post extraction. A new technique has been described here is a rotational pedicle combined epithelialized and connective tissue graft (RPC graft) adjunct with immediate guided tissue regeneration (GBR) procedure.

Results
We reviewed this technique through a case report and discuss it’s benefit in compare to other socket preservation procedures.

Conclusion
The main advantages of RPC graft would be summarized as follows: stable primary closure during bone remodeling, saving or crating sufficient vestibular depth, making adequate keratinized gingiva on the buccal surface, and being esthetically pleasant.
Molecular evidence of Culex pipiens form molestus and hybrids pipiens/molestus in Morocco, North Africa
Authors: Amraoui Fadila , Tijane Mhamed , Sarih Mhammed , Failloux Anna-Bella 
Published: April 2012

Abstract

Background

Culex pipiens L. is the most widespread mosquito vector in temperate regions including North Africa. Cx. pipiens has two recognized forms or biotypes; pipiens and molestus are morphologically indistinguishable with distinct behavior and physiology that may influence their vectorial status. In our study, we prospected for the different forms of Cx. pipiens in Morocco.

Methods

Cx. pipiens larvae were collected in 9 sites throughout Morocco during summer 2010 and reared until imago stage. Cx. pipiens was identified using diagnostic primers designed for the flanking region of microsatellite CQ11.

Results
We established the presence of both forms of Cx. pipiens and their hybrids in Morocco.

Conclusions
Molecular identification provides the first evidence of the presence of Cx. pipiens form molestus in Morocco and hybrids between pipiens and molestus forms in North Africa. The epidemiological implications of our findings are discussed.
Synovial cytokine expression in psoriatic arthritis and associations with lymphoid neogenesis and clinical features
Authors: Celis Raquel , Planell Núria , Fernández-Sueiro José L , Sanmartí Raimon , Ramírez Julio , González-Álvaro Isidoro , Pablos José L , Cañete Juan D 
Published: April 2012

Abstract
Introduction
Psoriatic arthritis (PsA) is an autoantibody-negative immune-mediated disease in which synovial lymphoid neogenesis (LN) occurs. We determined whether LN is associated with specific patterns of inflammatory cytokine expression in paired synovial tissue (ST) and fluid (SF) samples and their potential correlation with the clinical characteristics of PsA.

Methods
ST and paired SF samples were obtained from the inflamed knee of PsA patients. ST samples were immunostained with CD3 (T cell), CD20 (B cell), and MECA-79 (high endothelial vessels). Total ST mRNA was extracted, and the gene expression of 21 T-cell-derived and proinflammatory cytokines were measured with quantitative real-time PCR. SF concentrations of Th1, Th2, Th17, and proinflammatory cytokines were determined with the Quantibody Human Th17 Array. Clinical and biologic data were collected at inclusion and after a median of 27 months of follow-up.

Results
Twenty (43.5%) of 46 patients had LN. Only two genes showed differences (Wilcoxon test, P < 0.06) in ST between LN-positive and LN-negative patients: interleukin-23A (IL-23A) (P = 0.058) and transforming growth factor-beta (TGF-β1) (P = 0.050). IL-23A expression was higher, and TGF-β1 expression was lower in LN-positive patients. ST IL-15 mRNA showed a nonsignificant trend toward higher expression in LN-positive patients, and SF IL-15 protein levels were significantly higher in LN-positive patients (P = 0.002). In all PsA patients, IL-23A mRNA expression correlated with C-reactive protein (CRP) (r = 0.471; P = 0.001) and swollen-joint count (SJC) (r = 0.350; P = 0.018), whereas SF levels of IL-6 and CC chemokine-ligand 20 (CCL-20) correlated with CRP levels (r = 0.377; P = 0.014 and r = 0.501; P < 0.0001, respectively).

Conclusions
These findings suggest differences in the cytokine profile of PsA patients with LN, with a higher expression of IL-23A and IL-15 and a lower expression of TGF-β1. In the entire group of patients, IL-23 ST expression and CCL20 SF levels strongly correlated with markers of disease activity. This cytokine pattern was not accompanied by gross clinical or biologic differences between LN-positive and -negative patients. Taken together, these results suggest a role of the IL-17/IL-23 cytokine axis in synovial LN in PsA.
The interferon type I signature towards prediction of non-response to rituximab in rheumatoid arthritis patients
Authors: Raterman Hennie G , Vosslamber Saskia , de Ridder Sander , Nurmohamed Michael T , Lems Willem F , Boers Maarten , van de Wiel Mark , Dijkmans Ben AC , Verweij Cornelis L , Voskuyl Alexandre E 
Published: April 2012

Abstract
Introduction
B cell depletion therapy is efficacious in rheumatoid arthritis (RA) patients failing on tumor necrosis factor (TNF) blocking agents. However, approximately 40% to 50% of rituximab (RTX) treated RA patients have a poor response. We investigated whether baseline gene expression levels can discriminate between clinical non-responders and responders to RTX.

Methods
In 14 consecutive RA patients starting on RTX (test cohort), gene expression profiling on whole peripheral blood RNA was performed by Illumina® HumanHT beadchip microarrays. Supervised cluster analysis was used to identify genes expressed differentially at baseline between responders and non-responders based on both a difference in 28 joints disease activity score (ΔDAS28 < 1.2) and European League against Rheumatism (EULAR) response criteria after six months RTX. Genes of interest were measured by quantitative real-time PCR and tested for their predictive value using receiver operating characteristics (ROC) curves in an independent validation cohort (n = 26).

Results
Genome-wide microarray analysis revealed a marked variation in the peripheral blood cells between RA patients before the start of RTX treatment. Here, we demonstrated that only a cluster consisting of interferon (IFN) type I network genes, represented by a set of IFN type I response genes (IRGs), that is, LY6E, HERC5, IFI44L, ISG15, MxA, MxB, EPSTI1 and RSAD2, was associated with ΔDAS28 and EULAR response outcome (P = 0.0074 and P = 0.0599, respectively). Based on the eight IRGs an IFN-score was calculated that reached an area under the curve (AUC) of 0.82 to separate non-responders from responders in an independent validation cohort of 26 patients using Receiver Operator Characteristics (ROC) curves analysis according to ΔDAS28 < 1.2 criteria. Advanced classifier analysis yielded a three IRG-set that reached an AUC of 87%. Comparable findings applied to EULAR non-response criteria.

Conclusions
This study demonstrates clinical utility for the use of baseline IRG expression levels as a predictive biomarker for non-response to RTX in RA.
Role of common mental and physical disorders in partial
disability around the world
Authors: Bruffaerts Ronny , Vilagut Gemma , Demyttenaere Koen , Alonso Jordi , AlHamzawi Ali , Andrade Laura Helena , Benjet Corina , Bromet Evelyn , Bunting Brendan , de Girolamo Giovanni , Florescu Silvia , Gureje Oye , Haro Josep Maria , He Yanling , Hinkov Hristo , Hu Chiyi , Karam Elie G. , Lepine Jean-Pierre , Levinson Daphna , Matschinger Herbert , Nakane Yoshibumi , Ormel Johan , Posada-Villa Jose , Scott Kate M. , Varghese Matthew , Williams David R. , Xavier Miguel , Kessler Ronald C. 
Published: April 2012

BackgroundMental and physical disorders are associated with total disability, but
their effects on days with partial disability (i.e. the ability to
perform some, but not full-role, functioning in daily life) are not well
understood.AimsTo estimate individual (i.e. the consequences for an individual with a
disorder) and societal effects (i.e. the avoidable partial disability in
the society due to disorders) of mental and physical disorders on days
with partial disability around the world.MethodRespondents from 26 nationally representative samples (n
= 61 259, age 18+) were interviewed regarding mental and physical
disorders, and day-to-day functioning. The Composite International
Diagnostic Interview, version 3.0 (CIDI 3.0) was used to assess mental
disorders; partial disability (expressed in full day equivalents) was
assessed with the World Health Organization Disability Assessment
Schedule in the CIDI 3.0.ResultsRespondents with disorders reported about 1.58 additional disability days
per month compared with respondents without disorders. At the individual
level, mental disorders (especially post-traumatic stress disorder,
depression and bipolar disorder) yielded a higher number of days with
disability than physical disorders. At the societal level, the population
attributable risk proportion due to physical and mental disorders was 49%
and 15% respectively.ConclusionsMental and physical disorders have a considerable impact on partial
disability, at both the individual and at the societal level. Physical
disorders yielded higher effects on partial disability than mental
disorders.
Cognitive–behavioural group treatment for a range of functional somatic syndromes: Randomised trial
Authors: Schröder Andreas , Rehfeld Emma , Ørnbøl Eva , Sharpe Michael , Licht Rasmus W. , Fink Per 
Published: April 2012

BackgroundMany specialty-specific functional somatic syndrome diagnoses exist to describe people who are experiencing so-called medically unexplained symptoms. Although cognitive–behavioural therapy can be effective in the management of such syndromes, it is rarely available. A cognitive–behavioural therapy suitable for group treatment of people with different functional somatic syndromes could address this problem.AimsTo test the efficacy of a cognitive–behavioural therapy (Specialised Treatment for Severe Bodily Distress Syndromes, STreSS) designed for patients with a range of severe functional somatic syndromes.MethodA randomised controlled trial (clinicaltrials.gov, NCT00132197) compared STreSS (nine 3.5 h sessions over 4 months,n= 54) with enhanced usual care (management by primary care physician or medical specialist,n= 66). The primary outcome was improvement in aggregate score on subscales of the 36-item Short Form Health Survey (physical functioning, bodily pain and vitality) at 16 months.ResultsParticipants receiving STreSS had a greater improvement on the primary outcome (adjusted mean difference 4.0, 95% CI 1.4–6.6,P= 0.002) and on most secondary outcomes.ConclusionsIn the management of functional somatic syndromes, a cognitive–behavioural group treatment was more effective than enhanced usual care.
Effects of emotion perception training on mood in undergraduate students: randomised controlled trial
Authors: Penton-Voak Ian S. , Bate Helen , Lewis Glyn , Munafò Marcus R. 
Published: April 2012

SummaryWe investigated the effects of emotion perception training on depressive symptoms and mood in young adults reporting high levels of depressive symptoms (trial registration: ISRCTN02532638). Participants were randomised to an intervention procedure designed to increase the perception of happiness over sadness in ambiguous facial expressions or a control procedure, and completed self-report measures of depressive symptoms and mood. Those in the intervention condition had lower depressive symptoms and negative mood at 2-week follow-up, but there was no statistical evidence for a difference. There was some evidence for increased positive mood. Modification of emotional perception may lead to an increase in positive affect.
CPAP is required for cilia formation in neuronal cells
Authors: Wu Kuo-Sheng , Tang Tang K. 
Published: April 2012

Summary
The primary cilium is a microtubule-based structure protruded from the basal body analogous to the centriole. CPAP (centrosomal P4.1-associated protein) has previously been reported to be a cell cycle-regulated protein that controls centriole length. Mutations in CPAP cause primary microcephaly (MCPH) in humans. Here, using a cell-based system that we established to monitor cilia formation in neuronal CAD (Cath.a-differentiated) cells and hippocampal neurons, we found that CPAP is required for cilia biogenesis. Overexpression of wild-type CPAP promoted cilia formation and induced longer cilia. In contrast, an exogenously expressed CPAP-377EE mutant that lacks tubulin-dimer binding significantly inhibited cilia formation and caused cilia shortening. Furthermore, depletion of CPAP inhibited ciliogenesis and such effect was effectively rescued by expression of wild-type CPAP, but not by the CPAP-377EE mutant. Taken together, our results suggest that CPAP is a positive regulator of ciliogenesis whose intrinsic tubulin-dimer binding activity is required for cilia formation in neuronal cells.
Interactions between Type III receptor tyrosine phosphatases and growth factor receptor tyrosine kinases regulate tracheal tube formation in Drosophila
Authors: Jeon Mili , Scott Matthew P. , Zinn Kai 
Published: April 2012

Summary
The respiratory (tracheal) system of the Drosophila melanogaster larva is an intricate branched network of air-filled tubes. Its developmental logic is similar in some ways to that of the vertebrate vascular system. We previously described a unique embryonic tracheal tubulogenesis phenotype caused by loss of both of the Type III receptor tyrosine phosphatases (RPTPs), Ptp4E and Ptp10D. In Ptp4E Ptp10D double mutants, the linear tubes in unicellular and terminal tracheal branches are converted into bubble-like cysts that incorporate apical cell surface markers. This tube geometry phenotype is modulated by changes in the activity or expression of the epidermal growth factor receptor (Egfr) tyrosine kinase (TK). Ptp10D physically interacts with Egfr. Here we demonstrate that the Ptp4E Ptp10D phenotype is the consequence of the loss of negative regulation by the RPTPs of three growth factor receptor TKs: Egfr, Breathless and Pvr. Reducing the activity of any of the three kinases by tracheal expression of dominant-negative mutants suppresses cyst formation. By competing dominant-negative and constitutively active kinase mutants against each other, we show that the three RTKs have partially interchangeable activities, so that increasing the activity of one kinase can compensate for the effects of reducing the activity of another. This implies that SH2-domain downstream effectors that are required for the phenotype are likely to be able to interact with phosphotyrosine sites on all three receptor TKs. We also show that the phenotype involves increases in signaling through the MAP kinase and Rho GTPase pathways.
Funding health and social services for older people – a qualitative study of care recipients in the last year of life
Authors: Hanratty Barbara , Lowson Elizabeth , Holmes Louise , Grande Gunn , Addington-Hall Julia , Payne Sheila , Seymour Jane 
Published: April 2012

Objectives This study explores the views of older adults who are receiving health and social care at the end of their lives, on how services should be funded, and describes their health-related expenditure. Design Qualitative interview study Setting North West England Participants 30 people aged 69–93 years, diagnosed with lung cancer, heart failure or stroke and judged by health professionals to be in their last year of life. Sixteen participants lived in disadvantaged areas. Main outcome measures Views of older adults on funding of services. Results Participants expressed a belief in an earned entitlement to services funded from taxation, based on a broad sense of being a good citizen. Irrespective of social background, older people felt that those who could afford to pay for social care, should do so. Sale of assets and use of children's inheritance to fund care was widely perceived as an injustice. The costs of living with illness are a burden, and families are filling many of the gaps left by welfare provision. People who had worked in low-wage occupations were most concerned to justify their current acceptance of services, and distance themselves from what they described as welfare ‘spongers’ or ‘layabouts.’ Conclusions There is a gap between the health and social care system that older adults expect and what may be provided by a reformed welfare state at a time of financial stringencies. The values that underpinned the views expressed – mutuality, care for the most needy, and the importance of working to contribute to society – are an important contribution to the debate on welfare funding.
Dual specificity phosphatase 6 as a predictor of invasiveness in papillary thyroid cancer
Authors: Lee Jung Uee , Huang Songmei , Lee Min Hee , Lee Seong Eun , Ryu Min Jeong , Kim Soung Jung , Kim Yong Kyung , Kim Seul Young , Joung Kyong Hye , Kim Jin Man , Shong Minho , Jo Young Suk 
Published: April 2012

ObjectiveThe genetic mutations causing the constitutive activation of MEK/ERK have been regarded as an initiating factor in papillary thyroid carcinoma (PTC). The ERK-specific dual specificity phosphatase 6 (DUSP6) is part of the ERK-dependent transcriptional output. Therefore, the coordinated regulation of the activities of ERK kinases and DUSP6 may need to be reestablished to make new balances in PTC.MethodsTo investigate the role of DUSP6 in the regulation of ERK1/2 (MAPK3/1)-dependent transcription, 42 benign neoplasms and 167 PTCs were retrospectively analyzed by immunohistochemistry with dideoxy sequencing to detectBRAFV600Emutation.ResultsThe expressions of totalERK1/2,DUSP6,c-Fos(FOS),c-Myc(MYC), cyclin D1, andPCNAwere markedly increased in PTC compared with those in benign neoplasms. However, phospho-ERK1/2 was detected in only eight (4.8%) cases out of 167 PTC samples. Unexpectedly, the staining intensity and nuclear localization of ERK1/2 were not affected by the presence or absence of theBRAFV600Emutation. However, the expressions ofc-FosandPCNAwere elevated inBRAFV600E-positive PTC compared with those inBRAFV600E-negative PTC. Interestingly, the higher staining intensities of DUSP6 were associated with the level of totalERK1/2expression (P=0.04) and with high-risk biological features such as age (P=0.05), tumor size (P=0.01), and extrathyroidal extension (linear by linear association,P=0.02). In addition, DUSP6 silencing significantly decreased the cell viability and migration rate of FRO cells.ConclusionsThe coordinated upregulation of total ERK1/2 and its phosphatase, DUSP6, is related to bare detection of phospho-ERK1/2 in PTC regardless ofBRAFV600Emutation status. A link betweenDUSP6expression and high-risk features of PTC suggested thatDUSP6is an important independent factor affecting the signaling pathways in established PTC.
Characteristics of Patients Seeking Outpatient Rehabilitation for Pelvic-Floor Dysfunction
Authors: Wang Ying-Chih , Hart Dennis L. , Mioduski Jerome E. 
Published: April 2012

BackgroundPelvic-floor dysfunction (PFD) affects a substantial proportion of individuals, especially women.ObjectiveThe purposes of this study were: (1) to describe the characteristics of individuals with disorders associated with PFD who were seeking outpatient physical therapy services and (2) to identify the prevalence of specific pelvic-floor disorders in the group.DesignThis was a prospective, longitudinal, cohort study of 2,452 patients (mean age=50 years, SD=16, range=18–91) being treated in 109 outpatient physical therapy clinics in 26 states (United States) for their PFD.MethodsThis study examined patient demographic variables and summarized patient self-reported responses to questions related to urinary and bowel functioning at admission prior to receiving the therapy for their PFD disorders.ResultsPatients primarily were female (92%), were under 65 years of age (39%: 18 to &lt;45 years; 39%: 45 to &lt;65 years; 21%: 65 years or older), and had chronic symptoms (74%). Overall, 67% of the patients reported that they had urinary problems, 27% reported bowel problems, and 39% had pelvic pain. Among those who had urinary or bowel disorders, 32% and 54% reported leakage and constipation, respectively, as their only problem. Among patients who had pelvic pain, most (56%) reported that the pain was in the abdominal area. Combinations of urinary, bowel, or pelvic-floor pain disorders occurred in 31% of the patients.LimitationsBecause this study was a secondary analysis of data collected prospectively, the researchers were not in control of the data collection procedure. Missing data were common.ConclusionsData suggested most patients with PFD receiving outpatient physical therapy services were female, younger than 65 years, and had disorders lasting for more than 90 days. Combinations of urinary, bowel, or pelvic-floor pain disorders were not uncommon.