bluuebunny/crossref_metadata_2025_split huggingface dataset:
circSORBS1 inhibits lung cancer progression by sponging miR-6779-5p and directly binding RUFY3 mRNA
Authors: Xu Haotian , Zheng Yue , Wu Jiaxi , Zhang Ruirui , Zhao Qingyun , Chen Sixian , Peng Wenyi , Cai Dunyu , Gao Yihong , Chen Xingcai , Li Deqing , yuan Shengyi , Li Gang , Nan Aruo 
Published: June 2024

AbstractLung cancer is the primary cause of cancer-related death worldwide, and its global incidence and mortality rates remain high. The differential expression of circular RNAs (circRNAs) can affect the development of cancer, but the mechanisms by which circRNAs regulate lung cancer progression remain unclear. In this study, we identified circSORBS1, a circRNA that has not been previously described in lung cancer and is significantly underexpressed in lung cancer tissues, blood and cell lines, and the low expression of circSORBS1 correlated with tumour grade and prognosis. In vitro and in vivo functional experiments revealed that circSORBS1 overexpression inhibited cell proliferation and migration while enhancing apoptosis. Mechanistically, circSORBS1 acts as a sponge for miR-6779-5p, indirectly inhibiting RUFY3 mRNA degradation. Simultaneously, it binds to RUFY3 mRNA to enhance its stability. This dual regulatory mechanism leads to an increase in RUFY3 protein levels, which ultimately activates the YWHAE/BAD/BCL2 apoptotic signalling pathway and suppresses lung cancer progression. Our findings not only increase the knowledge about the regulatory pattern of circRNA expression but also provide new insights into the mechanisms by which circRNAs regulate lung cancer development.
The role of hematological parameters in asymptomatic and non-severe cases of Omicron variant infection
Authors: Ben Suqin , Gao Fengying , Xu Ziheng , Zhang Rulin , Zhang Xingyi , Wang Ning , Zhang Min , Hou Lili 
Published: June 2024

Abstract
Background
Omicron variants are currently the predominant circulating lineage worldwide and most cases are mild or asymptomatic. The Omicron variant is characterized by high transmissibility and immune evasion. Early identification of Omicron cases in clinical settings is crucial for controlling its spread. Previous studies have indicated that changes in hematological parameters can be used to predict the severity of coronavirus disease 2019 (COVID-19). However, the role of hematological parameters in non-severe and asymptomatic cases remains unknown. This study aimed to investigate the role of hematological parameters in non-severe and asymptomatic Omicron variant infections.

Methods
Hematological parameters and results were analyzed and compared in symptomatic (n = 356) and asymptomatic (n = 171) groups respectively, and between these two groups with positive COVID-19 tests. The utility of hematological parameters for predicting positive COVID-19 tests was analyzed using receiver operating characteristic curves.

Results
Individuals with non-severe cases exhibited decreased levels of platelets, lymphocytes, eosinophils, basophils, lymphocytes (%), eosinophils (%), and basophils (%), while exhibiting elevated counts of monocytes, neutrophils (%), monocytes (%), neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio (PLR), and C-reactive protein (CRP) when compared to suspected cases or asymptomatic carriers. In asymptomatic patients, positive carriers had lower leukocyte, neutrophil, and lymphocyte counts but higher monocyte, monocyte (%), PLR, and CRP levels than negative carriers. Basophil counts combined with lymphocytes or the PLR demonstrated a more significant predictive value in screening non-severe cases earlier compared to other parameters. The combined assessment of the monocyte (%) and the PLR had the highest area under the curve for diagnosing asymptomatic carriers.

Conclusions
Circulating basophils, alone or in combination with other hematological parameters, may be used as efficient biomarkers for early screening of non-severe Omicron cases.
Non-destructive wood identification using X-ray µCT scanning: which resolution do we need?
Authors: Dierickx Sofie , Genbrugge Siska , Beeckman Hans , Hubau Wannes , Kibleur Pierre , Van den Bulcke Jan 
Published: June 2024

Abstract
Background
Taxonomic identification of wood specimens provides vital information for a wide variety of academic (e.g. paleoecology, cultural heritage studies) and commercial (e.g. wood trade) purposes. It is generally accomplished through the observation of key anatomical features. Classic methodologies mostly require destructive sub-sampling, which is not always acceptable. X-ray computed micro-tomography (µCT) is a promising non-destructive alternative since it allows a detailed non-invasive visualization of the internal wood structure. There is, however, no standardized approach that determines the required resolution for proper wood identification using X-ray µCT. Here we compared X-ray µCT scans of 17 African wood species at four resolutions (1 µm, 3 µm, 8 µm and 15 µm). The species were selected from the Xylarium of the Royal Museum for Central Africa, Belgium, and represent a wide variety of wood-anatomical features.

Results
For each resolution, we determined which standardized anatomical features can be distinguished or measured, using the anatomical descriptions and microscopic photographs on the Inside Wood Online Database as a reference. We show that small-scale features (e.g. pits and fibres) can be best distinguished at high resolution (especially 1 µm voxel size). In contrast, large-scale features (e.g. vessel porosity or arrangement) can be best observed at low resolution due to a larger field of view. Intermediate resolutions are optimal (especially 3 µm voxel size), allowing recognition of most small- and large-scale features. While the potential for wood identification is thus highest at 3 µm, the scans at 1 µm and 8 µm were successful in more than half of the studied cases, and even the 15 µm resolution showed a high potential for 40% of the samples.

Conclusions
The results show the potential of X-ray µCT for non-destructive wood identification. Each of the four studied resolutions proved to contain information on the anatomical features and has the potential to lead to an identification. The dataset of 17 scanned species is made available online and serves as the first step towards a reference database of scanned wood species, facilitating and encouraging more systematic use of X-ray µCT for the identification of wood species.
NPRL2 is required for proliferation of oncogenic Ras-transformed bronchial epithelial cells
Authors: Chuang Jing-Yuan , Kuo Hsiao-Hui , Wang Pei-Han , Su Chih-Jou , Yih Ling-Huei 
Published: June 2024

AbstractNitrogen permease regulator-like 2 (NPRL2/TUSC4) is known to exert both tumor-suppressing and oncogenic effects in different types of cancers, suggesting that its actions are context dependent. Here, we delineated the molecular and functional effects of NPRL2 in malignantly transformed bronchial epithelial cells. To do so, we depleted NPRL2 in oncogenic HRas-transduced and malignantly transformed human bronchial epithelial (BEAS2B), Ras-AI-T2 cells. Intriguingly, depletion of NPRL2 in these cells induced activation of mTORC1 downstream signaling, inhibited autophagy, and impaired Ras-AI-T2 cell proliferation both in vitro and in vivo. These results suggest that NPRL2 is required for oncogenic HRas-induced cell transformation. Depletion of NPRL2 increased levels of the DNA damage marker γH2AX, the cell cycle inhibitors p21 and p27, and the apoptosis marker cleaved-PARP. These NPRL2-depleted cells first accumulated at G1 and G2, and later exhibited signs of mitotic catastrophe, which implied that NPRL2 depletion may be detrimental to oncogenic HRas-transformed cells. Additionally, NPRL2 depletion reduced heat shock factor 1/heat shock element- and NRF2/antioxidant response element-directed luciferase reporter activities in Ras-AI-T2 cells, indicating that NPRL2 depletion led to the suppression of two key cytoprotective processes in oncogenic HRas-transformed cells. Overall, our data suggest that oncogenic HRas-transduced and malignantly transformed cells may depend on NPRL2 for survival and proliferation, and depletion of NPRL2 also induces a stressed state in these cells.
Clinical evaluation of laryngeal mask airways in video-assisted thoracic surgery: a meta-analysis of randomized controlled trials
Authors: Luo Kai , Chen Kaiming , Li Yu , Ji Yang 
Published: June 2024

Abstract
Background
Endotracheal intubation is often associated with postoperative complications such as sore throat discomfort and hoarseness, reducing patient satisfaction and prolonging hospital stays. Laryngeal mask airway (LMA) plays a critical role in reducing airway complications related to endotracheal intubation. This meta-analysis was performed to determine the efficacy and safety of LMA in video-assisted thoracic surgery (VATS).

Methods
The PubMed, Embase, Cochrane Library, Medline and Web of Science databases were searched for eligible studies from inception until October 5, 2023. Cochrane’s tool (RoB 2) was used to evaluate the possibility biases of RCTs. We performed sensitivity analysis and subgroup analysis to assess the robustness of the results.

Results
Seven articles were included in this meta-analysis. Compared with endotracheal intubation, there was no significant difference in the postoperative hospital stay (SMD = -0.47, 95% CI = -0.98–0.03, P = 0.06), intraoperative minimum SpO2 (SMD = 0.00, 95% CI = -0.49–0.49, P = 1.00), hypoxemia (RR = 1.00, 95% CI = 0.26–3.89, P = 1.00), intraoperative highest PetCO2 (SMD = 0.51, 95% CI = -0.12–1.15, P = 0.11), surgical field satisfaction (RR = 1.01, 95% CI = 0.98–1.03, P = 0.61), anesthesia time (SMD = -0.10, 95% CI = -0.30–0.10, P = 0.31), operation time (SMD = 0.06, 95% CI = -0.13–0.24, P = 0.55) and blood loss (SMD =- 0.13, 95% CI = -0.33–0.07, P = 0.21) in LMA group. However, LMA was associated with a lower incidence of throat discomfort (RR = 0.28, 95% CI = 0.17–0.48, P < 0.00001) and postoperative hoarseness (RR = 0.36, 95% CI = 0.16–0.81, P = 0.01), endotracheal intubation was found in connection with a longer postoperative awake time (SMD = -2.19, 95% CI = -3.49 – -0.89, P = 0.001).

Conclusion
Compared with endotracheal intubation, LMA can effectively reduce the incidence of throat discomfort and hoarseness post-VATS, and can accelerate the recovery from anesthesia. LMA appears to be an alternative to endotracheal intubation for some specific thoracic surgical procedures, and the efficacy and safety of LMA in VATS need to be further explored in the future.
Quality of life and activities of daily living one year after Coronary Artery Bypass Graft (CABG) surgery: a cross-sectional study
Authors: Emamzadehashemi Khashayar Rezvani , Khanghah Atefeh Ghanbari , Azizi Ali , Paryad Ezzat , Noveiri Marzieh Jahani Sayad 
Published: June 2024

Abstract
Background
Daily activities have been recommended to minimize the long-term complications of coronary artery bypass as one of the strategies to return to the normal activity level, the effectiveness of which needs further investigation. This study aims to determine the quality of life and activities of daily living one year after CABG.

Methods
This cross-sectional study was performed on 206 patients who had undergone CABG for more than one year in 2018 in the north of Iran. The research instrument was a questionnaire including five sections, Data were analyzed using descriptive statistics and Chi2, Mann–Whitney U, Kruskal–Wallis tests, and a Logistic regression model.

Result
The mean score of quality of life was 31.7 ± 2.04 of 12 to 48 possible scores. About Activities of Daily Living results showed 99.5% and 84.7% of the samples needed help with many of these activities respectively. The mean score of quality of life was significantly different based on sex (p < 0.018) and instrumental activity of daily living (p < 0.0001). A logistic regression model was used to determine the factors related to quality of life. The final model showed cross-clamp duration (OR = 0.33,p = 0.014), length of stay(LOS)in the intensive care unit(OR = 0.42,p = 0.05), and instrumental activities of daily living (OR = 0.08,p = 0.001) predicted patients' quality of life one year after coronary artery bypass grafting.

Conclusion
Although more than half of the samples had a good average quality of life score, due to the lack of definitive treatment for coronary artery disease, it is suggested to consider predictive variables to help plan to improve the quality of life of these patients.
High risk Langerhans cell histiocytosis in children: the role of salvage in improving the outcome. A single center experience
Authors: Sedky Mohamed Sedky M. , Hamoda Asmaa , Taha Hala , Zaky Iman , Hassanain Omayma , ElHaddad Alaa 
Published: June 2024

Abstract
Background
In pediatric multi-system high risk organs (RO +) Langerhans cell histiocytosis (LCH), failing 1st line treatment has the highest mortality. We aim to present the outcome of failure of 1st line whether due to disease progression (DP) at end of induction or reactivation (REA) after initial better status response.

Patients and methods
Sixty-seven RO + LCH patients with hemopoietic, hepatic or splenic involvement, treated between 2007 and 2019 were retrospectively analyzed. The median follow-up (IQR) is 6 years (4–8.8 y).They were subjected to 2 eras of treatment; one with salvage by 2-Cda based regimen (2-CdABR) and another without.

Results
Of 67 patients, M/F 40/27, median age 1.74 y (0.2–10 y), 42 failed 1st line (62.7%). Of them DP n = 22 (52%) and REA n = 20 (48%). Of those with DP, 9/22 patients received 2-CdABR, where 5 survived in better status. While the remaining 13 did not receive 2-CdABR and all of them died. Otherwise, of those with REA, 12/20 reactivated on RO + mode. Of them, 8/12 received 2-CdABR, where only one survived in better status and the remaining 4 received vinblastine-based regimen,where 2 died and 2 were rescued. RO + 5-year overall survival (OS) was 65% (CI 95% 54 -78) while the event free survival (EFS) 36% (26.3—50.1). The OS of DP 27% (14–54) versus REA 67% (49–93) p 0.004. OS of DP with 2-CdABR 56% (31–97.7) versus 8% without (2–51), p < 0.001. While OS of REA with 2-CdABR 38% (13–100) versus 74% without (53–100) p 0.7.

Conclusion
Survival of RO + remains limited. Failure of 1st line in RO + due to DP carries worse prognosis in relation to REA. In DP those who were not salvaged by 2-CdABR, showed dismal outcome. This could not be shown when applied in REA.
scHolography: a computational method for single-cell spatial neighborhood reconstruction and analysis
Authors: Fu Yuheng C. , Das Arpan , Wang Dongmei , Braun Rosemary , Yi Rui 
Published: June 2024

AbstractSpatial transcriptomics has transformed our ability to study tissue complexity. However, it remains challenging to accurately dissect tissue organization at single-cell resolution. Here we introduce scHolography, a machine learning-based method designed to reconstruct single-cell spatial neighborhoods and facilitate 3D tissue visualization using spatial and single-cell RNA sequencing data. scHolography employs a high-dimensional transcriptome-to-space projection that infers spatial relationships among cells, defining spatial neighborhoods and enhancing analyses of cell–cell communication. When applied to both human and mouse datasets, scHolography enables quantitative assessments of spatial cell neighborhoods, cell–cell interactions, and tumor-immune microenvironment. Together, scHolography offers a robust computational framework for elucidating 3D tissue organization and analyzing spatial dynamics at the cellular level.
Non-orthogonal multiple access-based MEC for energy-efficient task offloading in e-commerce systems
Authors: Zheng Xiao , Tahir Muhammad , Aurangzeb Khursheed , Anwar Muhammad Shahid , Aamir Muhammad , Farzan Ahmad , Ullah Rizwan 
Published: June 2024

AbstractMobile edge computing (MEC) reduces the latency for end users to access applications deployed at the edge by offloading tasks to the edge. With the popularity of e-commerce and the expansion of business scale, server load continues to increase, and energy efficiency issues gradually become more prominent. Computation offloading has received widespread attention as a technology that effectively reduces server load. However, how to improve energy efficiency while ensuring computing requirements is an important challenge facing computation offloading. To solve this problem, using non-orthogonal multiple access (NOMA) to increase the efficiency of multi-access wireless transmission, MEC supporting NOMA is investigated in the research. Computing resources will be divided into separate sub-computing that will be handled via e-commerce terminals or transferred to edge sides by reutilizing radio resources, we put forward a Group Switching Matching Algorithm Based on Resource Unit Allocation (GSM-RUA) algorithm that is multi-dimensional. To this end, we first formulate this task allocation problem as a long-term stochastic optimization problem, which we then convert to three short-term deterministic sub-programming problems using Lyapunov optimization, namely, radio resource allocation in a large timescale, computation resource allocating and splitting in a small-time frame. Of the 3 short-term deterministic sub-programming problems, the first sub-programming problem can be remodeled into a 1 to n matching problem, which can be solved using the block-shift-matching-based radio resource allocation method. The latter two sub-programming problems are then transformed into two continuous convex problems by relaxation and then solved easily. We then use simulations to prove that our GSM-RUA algorithm is superior to the state-of-the-art resource management algorithms in terms of energy consumption, efficiency and complexity for e-commerce scenarios.
Oro-dental manifestations of eating disorders: a systematic review
Authors: Valeriani Leoluca , Frigerio Francesco , Piciocchi Claudia , Piana Gabriela , Montevecchi Marco , Donini Lorenzo Maria , Mocini Edoardo 
Published: June 2024

Abstract
Background
Eating disorders (EDs) pose a significant risk to health, especially when not diagnosed early. For several years EDs and oral health has been extensively studied, and now it is quite clear the existence of a correlation between specific oral manifestations and these disorders. While these oral signs could potentially aid early diagnosis of EDs, their identification and the eventual establishment of a correlation is currently heavily limited to the clinician’s experience. The present systematic review critically examines existing literature, offering an updated overview of oro-dental manifestations associated with EDs.

Method
MEDLINE (via PubMed), Web of Science, Scopus, and grey literature were searched, and relevant epidemiological comparative studies were screened using the Rayyan software. No limitations have been imposed on the research regarding oro-dental outcomes, encompassing all medically diagnosed EDs. The quality of the studies was valuated using AXIS appraisal tool for cross-sectional studies.

Result
Out of 3990 studies, 32 fulfilled the eligibility criteria and were included in the synthesis. The identified eating disorders include Anorexia Nervosa, Bulimia Nervosa and/or Eating Disorders Not Otherwise Specified, predominantly among female subjects, primarily originating from Europe. The evaluated oro-dental outcomes include dental erosion, caries, saliva assessment, hygiene-periodontal parameters, and mucosal tissue appearance. The association with erosion is confirmed while gingival recession, dentinal hypersensitivity, salivary flow thresholds and aspects relating to oral pathology are receiving increasing support from emerging evidence.

Discussion
This trend emphasizes the critical role of the complete intraoral examination to detect significant oro-dental signs that may indicate the onset of an ED.
Altered brain expression and cerebrospinal fluid levels of TIMP4 in cerebral amyloid angiopathy
Authors: Jäkel Lieke , De Kort Anna M. , Stellingwerf Arno , Hernández Utrilla Carla , Kersten Iris , Vervuurt Marc , Vermeiren Yannick , Küsters Benno , Schreuder Floris H. B. M. , Klijn Catharina J. M. , Kuiperij H. Bea , Verbeek Marcel M. 
Published: June 2024

AbstractCerebral amyloid angiopathy (CAA) is a highly prevalent and progressive pathology, involving amyloid-β (Aβ) deposition in the cerebral blood vessel walls. CAA is associated with an increased risk for intracerebral hemorrhages (ICH). Insight into the molecular mechanisms associated with CAA pathology is urgently needed, to develop additional diagnostic tools to allow for reliable and early diagnosis of CAA and to obtain novel leads for the development of targeted therapies. Tissue inhibitor of matrix metalloproteinases 4 (TIMP4) is associated with cardiovascular functioning and disease and has been linked to vascular dementia. Using immunohistochemistry, we studied occipital brain tissue samples of 57 patients with CAA (39 without ICH and 18 with ICH) and 42 controls, and semi-quantitatively assessed expression levels of TIMP4. Patients with CAA had increased vascular expression of TIMP4 compared to controls (p < 0.001), and in these patients, TIMP4 expression correlated with CAA severity (τb = 0.38; p = 0.001). Moreover, TIMP4 expression was higher in CAA-ICH compared to CAA-non-ICH cases (p = 0.024). In a prospective cross-sectional study of 38 patients with CAA and 37 age- and sex-matched controls, we measured TIMP4 levels in cerebrospinal fluid (CSF) and serum using ELISA. Mean CSF levels of TIMP4 were decreased in patients with CAA compared to controls (3.36 ± 0.20 vs. 3.96 ± 0.22 ng/ml, p = 0.033), whereas median serum levels were increased in patients with CAA (4.51 ng/ml [IQR 3.75–5.29] vs 3.60 ng/ml [IQR 3.11–4.85], p-9.013). Moreover, mean CSF TIMP4 levels were lower in CAA patients who had experienced a symptomatic hemorrhage compared to CAA patients who did not (2.13 ± 0.24 vs. 3.57 ± 0.24 ng/ml, p = 0.007). CSF TIMP4 levels were associated with CSF levels of Aβ40 (spearman r (rs) = 0.321, p = 0.009). In summary, we show that TIMP4 is highly associated with CAA and CAA-related ICH, which is reflected by higher levels in the cerebral vasculature and lower levels in CSF. With these findings we provide novel insights into the pathophysiology of CAA, and more specifically in CAA-associated ICH.