bluuebunny/crossref_metadata_2025_split huggingface dataset:
Genotypic relatedness and phenotypic characterization of Staphylococcus schleiferi subspecies in clinical samples from dogs
Authors: Cain Christine L. , Morris Daniel O. , O'Shea Kathleen , Rankin Shelley C. 
Published: January 2011

Abstract
Objective—To assess the degree of biological similarity (on the basis of genotype determined via pulsed-field gel electrophoresis [PFGE]) between isolates of 2 Staphylococcus schleiferi subspecies (S schleiferi subsp coagulans and S schleiferi subsp schleiferi) in clinical samples obtained from dogs.
Sample Population—161 S schleiferi isolates from 160 canine patients.
Procedures—A commercial microbiology identification system was used to identify each isolate as S schleiferi. Isolates underwent slide and tube coagulase testing and antimicrobial susceptibility testing. A mecA PCR assay and a latex agglutination test for penicillin-binding protein 2a (PBP2a) were also performed on each isolate. Clonal clusters with a similarity cutoff value of 80% were identified via PFGE.
Results—Of the 161 isolates, 61 (38%), 79 (49%), and 21 (13%) were obtained from cutaneous sites, ears, and other sites, respectively; 110 (68%) were coagulase negative, and 51 (32%) were coagulase positive. Among the coagulase-negative and coagulase-positive isolates, 65% (71/110) and 39% (20/51) were oxacillin resistant, respectively. All oxacillin-resistant isolates yielded positive results via mecA PCR assay and PBP2a latex agglutination testing. Via PFGE, 15 major clusters and 108 individual pulsed-field profiles were identified. Oxacillin-resistant and oxacillin-susceptible isolates clustered separately. Clonal clusters were heterogeneous and contained representatives of both subspecies.
Conclusions and Clinical Relevance—Coagulase-positive and coagulase-negative isolates were not genotypically distinct and may represent a single S schleiferi sp with variable coagulase production, rather than 2 biologically distinct subspecies. Further studies are needed to characterize clinical or epidemiological differences associated with infections with coagulase-positive and coagulase-negative S schleiferi in dogs.
Intraobserver and interobserver agreement for results of low-field magnetic resonance imaging in dogs with and without clinical signs of disk-associated wobbler syndrome
Authors: De Decker Steven , Gielen Ingrid M. V. L. , Duchateau Luc , Lang Johann , Dennis Ruth , Corzo-Menéndez Nuria , van Bree Henri J. J. , Van Soens Iris , Binst Dominique H. A. R. , Waelbers Tim , Van Ham Luc M. L. M. 
Published: January 2011

Abstract
Objective—To determine interobserver and intraobserver agreement for results of low-field magnetic resonance imaging (MRI) in dogs with and without disk-associated wobbler syndrome (DAWS).
Design—Validation study.
Animals—21 dogs with and 23 dogs without clinical signs of DAWS.
Procedures—For each dog, MRI of the cervical vertebral column was performed. The MRI studies were presented in a randomized sequence to 4 board-certified radiologists blinded to clinical status. Observers assessed degree of disk degeneration, disk-associated and dorsal compression, alterations in intraspinal signal intensity (ISI), vertebral body abnormalities, and new bone formation and categorized each study as originating from a clinically affected or clinically normal dog. Interobserver agreement was calculated for 44 initial measurements for each observer. Intraobserver agreement was calculated for 11 replicate measurements for each observer.
Results—There was good interobserver agreement for ratings of disk degeneration and vertebral body abnormalities and moderate interobserver agreement for ratings of disk-associated compression, dorsal compression, alterations in ISI, new bone formation, and suspected clinical status. There was very good intraobserver agreement for ratings of disk degeneration, disk-associated compression, alterations in ISI, vertebral body abnormalities, and suspected clinical status. There was good intraobserver agreement for ratings of dorsal compression and new bone formation. Two of 21 clinically affected dogs were erroneously categorized as clinically normal, and 4 of 23 clinically normal dogs were erroneously categorized as clinically affected.
Conclusions and Clinical Relevance—Results suggested that variability exists among observers with regard to results of MRI in dogs with DAWS and that MRI could lead to false-positive and false-negative assessments.
Transcranial magnetic stimulation in Doberman Pinschers with clinically relevant and clinically irrelevant spinal cord compression on magnetic resonance imaging
Authors: De Decker Steven , Van Soens Iris , Duchateau Luc , Gielen Ingrid M. V. L. , van Bree Henri J. J. , Binst Dominique H. A. R. , Waelbers Tim , Van Ham Luc M. L. M. 
Published: January 2011

Abstract
Objective—To evaluate the use of transcranial magnetic stimulation for differentiating between clinically relevant and clinically irrelevant cervical spinal cord compression on magnetic resonance imaging (MRI).
Design—Validation study.
Animals—Clinically normal Doberman Pinschers without (n = 11) and with (6) spinal cord compression on MRI and 16 Doberman Pinschers with disk-associated wobbler syndrome (DAWS).
Procedures—After dogs were sedated, transcranial magnetic motor evoked potentials were recorded from the extensor carpi radialis muscle (ECRM) and cranial tibial muscle (CTM). Onset latencies and peak-to-peak amplitudes were measured. Magnetic resonance imaging was performed to identify spinal cord compression.
Results—There were significant differences in ECRM and CTM onset latencies between Doberman Pinschers with DAWS and each of the 2 groups of clinically normal dogs, but there were no significant differences in ECRM and CTM onset latencies between the 2 groups of clinically normal dogs. There were significant differences in CTM peak-to-peak amplitudes between Doberman Pinschers with DAWS and each of the 2 groups of clinically normal dogs, but there were no significant differences in ECRM peak-to-peak amplitudes among groups or in CTM peak-to-peak amplitudes between the 2 groups of clinically normal dogs. There was a significant correlation between severity of spinal cord compression and ECRM onset latency, CTM onset latency, and CTM peak-to-peak amplitude.
Conclusions and Clinical Relevance—Results suggested that transcranial magnetic stimulation may be a useful diagnostic tool to differentiate between clinically relevant and clinically irrelevant spinal cord compression identified on MRI alone.
Outcomes of cats undergoing surgical attenuation of congenital extrahepatic portosystemic shunts through cellophane banding: 9 cases (2000–2007)
Authors: Cabassu Julien , Seim Howard B. , MacPhail Catriona M. , Monnet Eric 
Published: January 2011

Abstract
Objective—To evaluate the long-term prognosis of cats with a congenital extrahepatic portosystemic shunt (CEPSS) attenuated through gradual occlusion with cellophane banding (CB).
Design—Retrospective case series.
Animals—9 cats with a CEPSS that was attenuated with CB.
Procedures—Medical records of cats surgically treated for CEPSS by means of CB from January 2000 through March 2007 were reviewed. Extracted data included preoperative clinical signs, medications, diagnostic results including serum bile acids concentrations, surgical technique, intraoperative and postoperative complications, and long-term follow-up information.
Results—2 cats that developed refractory seizures were euthanized within 3 days after the CB procedure. Seven of the 9 cats survived to 15 days after surgery. Four cats did not have any clinical signs of CEPSS at long-term follow up. At that time, 5 cats had a postprandial SBA concentration within reference limits and 1 cat had persistent ptyalism. One cat had biurate ammonium stones removed > 2 years after surgery. One cat was euthanized 105 days after surgery because of uncontrolled seizures. The 3-year survival rate was 66%.
Conclusions and Clinical Relevance—Uncontrolled seizure activity was the most common cause of death after CB. Long-term outcome for cats with CEPSS was fair to good after the procedure. Cats with a CEPSS surviving the immediate postoperative period had a fair to good long-term outcome. Cellophane banding without intraoperative attenuation appears to be an acceptable technique for gradual occlusion of a CEPSS in cats. Cats should be monitored closely for development of neurologic disorders in the postoperative period.
Evaluation of the association between fecal excretion of Mycobacterium avium subsp paratuberculosis and detection in colostrum and on teat skin surfaces of dairy cows
Authors: Pithua Patrick , Wells Scott J. , Godden Sandra M. 
Published: January 2011

Abstract
Objective—To evaluate the association between fecal excretion of Mycobacterium avium subsp paratuberculosis (MAP) by dairy cows in the periparturient period and detection of MAP DNA in colostrum specimens and on teat skin surfaces.
Design—Cross-sectional study.
Animals—112 Holstein cows.
Procedures—Fecal specimens were collected within 48 to 72 hours prior to parturition, and colostrum and teat swab specimens were collected immediately after parturition. Detection of MAP in fecal specimens was performed via microbial culture, and detection of MAP DNA in colostrum and teat swab specimens was achieved via a PCR assay targeting the genetic element ISMAP02. Logistic regression was used to model the relationship between MAP fecal shedding status and detection of MAP DNA in colostrum or teat swab specimens. Population attributable fractions for the proportion of colostrum and teat swab specimens containing MAP DNA were also calculated.
Results—The odds of detecting MAP DNA in colostrum or teat swab specimens in cows with MAP-positive (vs negative) fecal specimens were 2.02 and 1.87 respectively. Population attributable fractions estimates suggested that withholding colostrum from MAP-positive cows could reduce the odds of exposing calves to MAP in colostrum by 18.2%. Not permitting natural suckling by calves could reduce the odds of exposing calves to MAP on the teat surfaces of MAP-positive cows by 19.5%.
Conclusions and Clinical Relevance—Results underscored the need for strict adherence to practices that limit contact of calves with adult cows from the time of birth and promote hygienic colostrum handling to avoid possible contamination with MAP during colostrum harvest, storage, or feeding.
Natural history of Polish patients with mucopolysaccharidosis type VI
Authors: Jurecka Agnieszka , Rozdzynska Agnieszka , Marucha Jolanta , Czartoryska Barbara , Wegrzyn Grzegorz , Tylki-Szymanska Anna 
Published: December 2010

AbstractThe aim of the study was to describe the natural history, anthropometric features, range of motion (ROM) and molecular characteristics of Polish patients with mucopolysaccharidosis (MPS) VI. Clinical heterogeneity was observed and two major clinical phenotypes of the disease were distinguished, rapidly advancing and relatively attenuated. Two patients developed symptoms early in life presenting with short stature, significant skeletal malformations and other clinical abnormalities. In two other patients, height was only slightly decreased and MPS features developed later in the course of the disease. All patients had similar characteristics at the time of birth but showed significant differences in body proportions when compared with the healthy population. Differences between healthy and affected children increased with age and were reflected in phenotypes. Analysis of ROM showed impairments at multiple joints, although to a various degree in different patients. Restriction in upper extremity ROM was observed since the second year of life, while restriction in lower extremity ROM developed later and influenced stereotype of walking. These limitations intensified with the patients’ age, which made self-care more difficult or impossible. The molecular analysis revealed that the milder phenotype may be associated with the R152W mutation, which suggests a specific genotype-phenotype correlation.
OCT 4 immunohistochemistry in postpubertal cryptorchidism
Authors: Krhen Ivan , Kulis Tomislav , Coric Marijana , Knezevic Nikola , Marekovic Zvonimir , Kastelan Zeljko 
Published: December 2010

AbstractPatients with cryptorchidism are at an increased risk for germ cell testicular cancer. OCT 4 has been shown to be a sensitive and specific marker for some types of germ cell testicular cancer. We undertook this study to establish whether OCT 4 immunohistochemistry is a useful tool in the pathohistologic evaluation of postpubertal patients with cryptorchidism. Seventeen postpubertal patients underwent orchidectomy for cryptorchidism at our center since 1997. Immunohistochemical staining with OCT 4 was performed on these samples. Characteristic OCT 4 nuclear staining was positive in two patients. One patient was correctly diagnosed on previous pathohistological evaluation, while OCT4 immunohistochemical staining revealed previously unidentified intratubular germ cell neoplasia in the other patient. OCT 4 immunohistochemistry can be useful in diagnosing a testicular germ cell tumor in patients with cryptorchidism. If we consider a low number of postpubertal patients with cryptorchidism a benefit of immunohistochemical staining with OCT4, this could favor the use of OCT 4 staining in work-up of cryptorchidism.
Cardiogenic shock in myocardial infarction-results of in-hospital follow-up
Authors: Kawecki Damian , Morawiec Beata , Rybczyk Renata , Trzepaczyńska Zofia , Przywara-Chowaniec Brygida , Wojciechowska Celina , Fudal Marcin , Wilczewski Przemysław , Jacheć Wojciech , Nowalany-Kozielska Ewa 
Published: December 2010

AbstractThe purpose of this study was to present the outcomes of treatment of cardiogenic shock (CS) complicating acute myocardial infarction (AMI) among patients hospitalized from 1999 through 2006. The study enrolled 1003 patients. Group 1 comprised 87 patients presenting with AMI complicated with CS, whereas Group 2 comprised 916 patients presenting with AMI without CS symptoms. Determination of invasive treatment was according to standard guidelines. The endpoint comprised death, stroke, and reocclusion/reinfarction. Follow-up was confined to the intra-hospital period. CS was observed more frequently in cases of ST-elevation MI (STEMI) and right ventricular MI. The transportation and door-to-needle time were shorter in Group 1. CS patients were characterized by a more severe coronary artery disease, higher maximal creatinine kinase levels, lower global ejection fractions, and increased incidence of atrioventricular conduction disorders. The efficacy of percutaneous coronary intervention (PCI) was 82.26% in Group 1 and 95.03% in Group 2. Death occurred in 33.3% of CS patients and in 3.6% of AMI patients (p<0.0001). Our study proved that in a short-term follow-up, PCI is a procedure of high efficacy in CS patients. The short-term follow-up precluded a conclusion of statistically significant benefits from the shortening of the transportation and door-to-needle time.
Evaluation of CO2 Emissions from Energy Sources in Latvia
Authors: Blumberga D. , Veidenbergs I. , Gusca J. , Rosa M. 
Published: January 2011

Evaluation of CO2Emissions from Energy Sources in LatviaThe authors propose an empirical model for evaluation of the CO2emissions released from the energy generation sources as a function of the fuel types, energy efficiency of the technologies used, and the emission factors of the fuels. In the research, multifactor linear and nonlinear regression equations are employed. The developed model has been proved with the data of the energy generation sources taking part in the Latvian Emission Trading Scheme.
Ciliates on the Macrophytes in Industrially Heated Lakes (Kujawy Lakeland, Poland)
Authors: Babko R. , Fyda J. , Kuzmina T. , Hutorowicz A. 
Published: December 2010

Ciliates on the Macrophytes in Industrially Heated Lakes (Kujawy Lakeland, Poland)The ciliate assemblage on the macrophytes was examined in 2005 during the vegetation period in the Konińskie Lakes which are heating by post-cooling waters from thermal electric plants. As a result of changed temperature regimen the alien thermophilic macrophyteVallisneria spiralisis becoming increasingly common in the littoral zone. A total of 150 ciliate taxa belonging to 27 orders were found. Greater ciliate species diversity was found on architecturally complex, submerged forms such asCeratophyllum demersumandMyriophyllum spicatum. By contrast the ciliate compositions on emergent macrophytes with simple architecture in their submerged parts, such asTypha, Sparganium, orAcorus, were less species rich. Despite the simple architecture ofVallisnerialeaves, the ciliate diversity on them was high. The results show that replacement of native macrophytes by the alien formV. spiralisin heated lakes did not impoverish the ciliate diversity.
Hymenolepidoid Cestodes (Cyclophyllidae, Hymenolepidoidae) of Pratincole (Glareola Pratincola) from the South of Ukraine
Authors: Kornyushin V. , Greben O. 
Published: December 2010

Hymenolepidoid Cestodes (Cyclophyllidae, Hymenolepidoidae) of Pratincole (Glareola Pratincola) from the South of UkraineFour hymenolepidoid cestode speciesGlareolepis porale(Meggitt, 1927) Spassky, 1967;Wardium(s. l.)tauricumKornyushin et Greben, sp. n.;Wardium(s. l.) sp. n.; andEchinocotyloides longirostris(Rudolphi, 1819) Kornyushin, 1983 from pratincole from the Southern Ukraine are discribed. The morphology ofG. poraleis described in details. An interesting new observation is that testes are lacking in female proglottides ofG. porale; the structures described in literature as testes in hermaphroditic proglottides are, in fact, ovarian lobes. The new cestode speciesWardium(s. l.)tauricumsp. n. is described. It is characterised by aploparaksoid hooks, 13-15 μm long, and conical armed cirrus, 20-22 μm long. Another cestode species,Wardium(s. l.) sp., is identified to the generic level only. It differs from all the species of the genus in the size and armament of the cirrus; however, no scolex was present in the material studied.
Helminths of the Marbled Goby (Pomatoschistus Marmoratus) a Mediterranean Immigrant in the Black Sea Fauna
Authors: Kvach Yu. 
Published: December 2010

Helminths of the Marbled Goby (Pomatoschistus Marmoratus) a Mediterranean Immigrant in the Black Sea FaunaThe marbled goby,Pomatoschistus marmoratus(Risso, 1810), the Mediterranean immigrant in the Black Sea fauna, was examined for the metazoan parasites. In total, 12 parasite species were found to occurre. The component community consists of 7 trematode species, two nematodes, two acanthocephalans, and one cestode species. Two parasite species (Aphalloides coelomicolaandDichelyne minutus) were represented by both adults and larvae, four parasite species were presented by adults (Asymphylodora pontica, Paratimonia gobii, Acanthocephaloides propinquus, andTelosentis exiguus), and six species were presented by larvae (Bothriocephalus gregarius, Cryptocotyle concavum, C. lingua, Pygidiopsis genata, Timoniella imbutiforme, andContracaecum microcephalum).T. imbutiformehas the great tendency to join the infracommunity. The core of parasite fauna of the marbled goby consists of two specialists,A. coelomicolaandP. gobii, which immigrated to the Black Sea together with their hosts. The co-immigration is a result of co-evolution of the parasites' and host's life cycles.
First Record of Mites of the Family Stigmaeidae (Acari, Raphignathoidea) from Rovno Amber with Description of a New Species of the Genus Mediolata
Authors: Kuznetsov N. , Khaustov A. , Perkovsky E. 
Published: December 2010

First Record of Mites of the Family Stigmaeidae (Acari, Raphignathoidea) from Rovno Amber with Description of a New Species of the Genus Mediolata
A new species, Mediolata eocenica Kuznetsov, Khaustov et Perkovsky, sp. n., is described from the Late Eocene Rovno amber. It is the first fossil record of Stigmaeidae.
Cross-border Cultural Tourism in Europe: Drivers for Cross-border Travels for the Performing Arts
Authors: Tajtáková Mária , Kopaničová Janka , Francová Zuzana 
Published: December 2010

Cross-border Cultural Tourism in Europe: Drivers for Cross-border Travels for the Performing Arts
The aim of this paper is to examine the drivers for cross-border tourist exchanges between the two closest capitals in Europe - Bratislava (Slovakia) and Vienna (Austria). We focus on the field of cultural tourism in view of the cross-border visits to two major performing arts institutions - the Slovak National Theatre in Bratislava and the Staatsoper in Vienna. The basic question raised by the study is: What makes tourists travel for the performing arts? The paper presents partial results of a bigger research analyzing intercultural differences in perception of cultural products among different audiences.
Poisson Downward Continuation Solution by the Jacobi Method
Authors: Kingdon R. , Vaníček P. 
Published: January 2011

Poisson Downward Continuation Solution by the Jacobi MethodDownward continuation is a continuing problem in geodesy and geophysics. Inversion of the discrete form of the Poisson integration process provides a numerical solution to the problem, but because the B matrix that defines the discrete Poisson integration is not always well conditioned the solution may be noisy in situations where the discretization step is small and in areas containing large heights. We provide two remedies, both in the context of the Jacobi iterative solution to the Poisson downward continuation problem. First, we suggest testing according to the upward continued result from each solution, rather then testing between successive solutions on the geoid, so that choice of a tolerance for the convergence of the iterative method is more meaningful and intuitive. Second, we show how a tolerance that reflects the conditioning of the B matrix can regularize the solution, and suggest an approximate way of choosing such a tolerance. Using these methods, we are able to calculate a solution that appears regular in an area of Papua New Guinea having heights over 3200 m, over a grid with 1 arc-minute spacing, based on a very poorly conditioned B matrix.
Introducing the Nepal Journal of Epidemiology: A Pioneer in its Domain
Authors: Sathian Brijesh 
Published: December 2010

The Nepal Journal of Epidemiology is the first journal of its kind in Nepal. Our main objective in pioneering this journal is to attempt to provide a common platform for all researchers, particularly those doing epidemiological studies on prevailing public health problems in the community.DOI: 10.3126/nje.v1i1.4099Nepal Journal of Epidemiology 2010;1 (1):1-3