bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impact of Fluid Distribution and Petrophysics on Geophysical Signatures of CO2 Storage Sandstone Reservoirs
Authors: Falcon‐Suarez I. H. , Mangriotis M.‐D. , Papageorgiou G. , Mondol N. H. 
Published: May 2024

AbstractCarbon Capture and Storage (CCS) is a key element to achieving net‐zero energy challenge timely. CCS operations require the integration of geophysical data, such as seismic and electromagnetic surveys, numerical reservoir models and fluid flow simulations. However, the 10–100s m resolution of seismic imaging methods complicates the mapping of smaller scale rock heterogeneities, while borehole measurements commonly show large fluctuations at sub‐cm scales. In this study, we combine laboratory data, well‐logging, rock physics theories and a proof‐of‐concept time‐lapse seismic modeling to assess the effect of pore‐scale fluid distribution and petrophysical heterogeneities on the expected performance of whole‐reservoir CCS operations in deep saline aquifers, by analogy to the Aurora CCS site, North Sea. We monitored the elastic and electrical properties of three sandstone samples with slightly different physical and petrographic properties during carbon dioxide (CO2) flow‐through tests under equivalent in situ effective pressure. We inferred the CO2‐induced damage in the rocks from the variations of their hydromechanical properties. We found that the clay fraction, CO2‐clay chemical interactions, and porosity were the main factors affecting both the CO2 distribution in the samples and the hydromechanical response. We used seismic modeling of well‐log data and the laboratory results to estimate the reservoir‐scale time‐lapse seismic response to CO2 injection and to assess the effect of the rock heterogeneities in our interpretation. The results show that disregarding the effect of rock heterogeneities on the CO2‐brine fluids distribution can lead to significant misinterpretations of seismic monitoring surveys during CCS operations in terms of both CO2 quantification and distribution.
A new fabrication method for enhancing the yield of linear micromirror arrays assisted by temporary anchors
Authors: Xiao Xingchen , Mao Ting , Shi Yingchao , Zhou Kui , Hao Jia , Yu Yiting 
Published: May 2024

AbstractAs one of the most common spatial light modulators, linear micromirror arrays (MMAs) based on microelectromechanical system (MEMS) processes are currently utilized in many fields. However, two crucial challenges exist in the fabrication of such devices: the adhesion of silicon microstructures caused by anodic bonding and the destruction of the suspended silicon film due to residual stress. To solve these issues, an innovative processing method assisted by temporary anchors is presented. This approach effectively reduces the span of silicon microstructures and improves the Euler buckling limit of the silicon film. Importantly, these temporary anchors are strategically placed within the primary etching areas, enabling easy removal without additional processing steps. As a result, we successfully achieved wafer-level, high-yield manufacturing of linear MMAs with a filling factor as high as 95.1%. Demonstrating superior capabilities to those of original MMAs, our enhanced version boasts a total of 60 linear micromirror elements, each featuring a length-to-width ratio of 52.6, and the entire optical aperture measures 5 mm × 6 mm. The linear MMA exhibits an optical deflection angle of 20.4° at 110 Vdc while maintaining exceptional deflection flatness and uniformity. This study offers a viable approach for the design and fabrication of thin-film MEMS devices with high yields, and the proposed MMA is promising as a replacement for digital micromirror devices (DMDs, by TI Corp.) in fields such as spectral imaging and optical communication.
SPEED: an integrated, smartphone-operated, handheld digital PCR Device for point-of-care testing
Authors: Zhang Haoqing , Liu Xiaocheng , Wang Xinlu , Yan Zhiqiang , Xu Ying , Gaňová Martina , Řezníček Tomáš , Korabečná Marie , Neuzil Pavel 
Published: May 2024

AbstractThis study elaborates on the design, fabrication, and data analysis details of SPEED, a recently proposed smartphone-based digital polymerase chain reaction (dPCR) device. The dPCR chips incorporate partition diameters ranging from 50 μm to 5 μm, and these partitions are organized into six distinct blocks to facilitate image processing. Due to the superior thermal conductivity of Si and its potential for mass production, the dPCR chips were fabricated on a Si substrate. A temperature control system based on a high-power density Peltier element and a preheating/cooling PCR protocol user interface shortening the thermal cycle time. The optical design employs four 470 nm light-emitting diodes as light sources, with filters and mirrors effectively managing the light emitted during PCR. An algorithm is utilized for image processing and illumination nonuniformity correction including conversion to a monochromatic format, partition identification, skew correction, and the generation of an image correction mask. We validated the device using a range of deoxyribonucleic acid targets, demonstrating its potential applicability across multiple fields. Therefore, we provide guidance and verification of the design and testing of the recently proposed SPEED device.
Temporal dissociation between local and global functional adaptations of the maternal brain to childbirth: a longitudinal assessment
Authors: Lotter Leon D. , Nehls Susanne , Losse Elena , Dukart Juergen , Chechko Natalya 
Published: May 2024

AbstractThe maternal brain undergoes significant reorganization during birth and the postpartum period. However, the temporal dynamics of these changes remain unclear. Using resting-state functional magnetic resonance imaging, we report on local and global brain function alterations in 75 mothers in their first postpartum week, compared to 23 nulliparous women. In a subsample followed longitudinally for the next six months, we observed a temporal and spatial dissociation between changes observed at baseline (cluster mass permutation: pFWE < 0.05). Local activity and connectivity changes in widespread neocortical regions persisted throughout the studied time period (ANCOVAs vs. controls: pFDR < 0.05), with preliminary evidence linking these alterations to behavioral and psychological adaptations (interaction effect with postpartum time: uncorrected p < 0.05). In contrast, the initially reduced whole-brain connectivity of putamen-centered subcortical areas returned to control levels within six to nine weeks postpartum (linear and quadratic mixed linear models: pFDR < 0.05). The whole-brain spatial colocalization with hormone receptor distributions (Spearman correlations: pFDR < 0.05) and preliminary blood hormone associations (interaction effect with postpartum time: uncorrected p < 0.05) suggested that the postpartum restoration of progesterone levels may underlie this rapid normalization. These observations enhance our understanding of healthy maternal brain function, contributing to the identification of potential markers for pathological postpartum adaptation processes, which in turn could underlie postpartum psychiatric disorders.
Tumor biomarkers for diagnosis, prognosis and targeted therapy
Authors: Zhou Yue , Tao Lei , Qiu Jiahao , Xu Jing , Yang Xinyu , Zhang Yu , Tian Xinyu , Guan Xinqi , Cen Xiaobo , Zhao Yinglan 
Published: May 2024

AbstractTumor biomarkers, the substances which are produced by tumors or the body’s responses to tumors during tumorigenesis and progression, have been demonstrated to possess critical and encouraging value in screening and early diagnosis, prognosis prediction, recurrence detection, and therapeutic efficacy monitoring of cancers. Over the past decades, continuous progress has been made in exploring and discovering novel, sensitive, specific, and accurate tumor biomarkers, which has significantly promoted personalized medicine and improved the outcomes of cancer patients, especially advances in molecular biology technologies developed for the detection of tumor biomarkers. Herein, we summarize the discovery and development of tumor biomarkers, including the history of tumor biomarkers, the conventional and innovative technologies used for biomarker discovery and detection, the classification of tumor biomarkers based on tissue origins, and the application of tumor biomarkers in clinical cancer management. In particular, we highlight the recent advancements in biomarker-based anticancer-targeted therapies which are emerging as breakthroughs and promising cancer therapeutic strategies. We also discuss limitations and challenges that need to be addressed and provide insights and perspectives to turn challenges into opportunities in this field. Collectively, the discovery and application of multiple tumor biomarkers emphasized in this review may provide guidance on improved precision medicine, broaden horizons in future research directions, and expedite the clinical classification of cancer patients according to their molecular biomarkers rather than organs of origin.
Hyperpure chlorine dioxide versus chlorhexidine in intra-oral halitosis (ODOR trial) – protocol of a double-blinded, double-arm, parallel non-inferiority pilot randomized controlled trial
Authors: Szalai Eszter Ágnes , Teutsch Brigitta , Babay Viktória , Galvács Adél , Hegyi Péter , Hársfalvi Péter , Pál Róbert , Varga Gábor , Lohinai Zsolt M. , Kerémi Beáta 
Published: May 2024

Abstract
Introduction
Intra-oral halitosis (IOH) is the most common type of bad breath; its consequences impair quality of life. However, evidence-based treatment protocols and guidelines are lacking. Our aim is to investigate the effectiveness of chlorine dioxide as an applicable complementary treatment modality in IOH after tongue cleaning.

Methods and analysis
The ODOR trial will be a single-center, double-blinded, parallel-group, double-armed pilot randomized controlled trial with a non-inferiority design. The efficacy of hyperpure chlorine dioxide will be compared to chlorhexidine mouthwash. We plan to investigate the short-term effects of the intervention over a 3-h period. The primary endpoint will be changes in organoleptic test scores. At the end of the pilot investigation of the first 30 patients each, sample size calculation will be performed. If feasible, the investigators will continue the study by enrolling more patients.

Trial registration
The trial has been registered at ClinicalTrials.gov (NCT06219226).
CDK9 inhibition as an effective therapy for small cell lung cancer
Authors: Valdez Capuccino L. , Kleitke T. , Szokol B. , Svajda L. , Martin F. , Bonechi F. , Krekó M. , Azami S. , Montinaro A. , Wang Y. , Nikolov V. , Kaiser L. , Bonasera D. , Saggau J. , Scholz T. , Schmitt A. , Beleggia F. , Reinhardt H. C. , George J. , Liccardi G. , Walczak H. , Tóvári J. , Brägelmann J. , Montero J. , Sos M. L. , Őrfi L. , Peltzer N. 
Published: May 2024

AbstractTreatment-naïve small cell lung cancer (SCLC) is typically susceptible to standard-of-care chemotherapy consisting of cisplatin and etoposide recently combined with PD-L1 inhibitors. Yet, in most cases, SCLC patients develop resistance to first-line therapy and alternative therapies are urgently required to overcome this resistance. In this study, we tested the efficacy of dinaciclib, an FDA-orphan drug and inhibitor of the cyclin-dependent kinase (CDK) 9, among other CDKs, in SCLC. Furthermore, we report on a newly developed, highly specific CDK9 inhibitor, VC-1, with tumour-killing activity in SCLC. CDK9 inhibition displayed high killing potential in a panel of mouse and human SCLC cell lines. Mechanistically, CDK9 inhibition led to a reduction in MCL-1 and cFLIP anti-apoptotic proteins and killed cells, almost exclusively, by intrinsic apoptosis. While CDK9 inhibition did not synergise with chemotherapy, it displayed high efficacy in chemotherapy-resistant cells. In vivo, CDK9 inhibition effectively reduced tumour growth and improved survival in both autochthonous and syngeneic SCLC models. Together, this study shows that CDK9 inhibition is a promising therapeutic agent against SCLC and could be applied to chemo-refractory or resistant SCLC.
Information-based TMS to mid-lateral prefrontal cortex disrupts action goals during emotional processing
Authors: Lapate R. C. , Heckner M. K. , Phan A. T. , Tambini A. , D’Esposito M. 
Published: May 2024

AbstractThe ability to respond to emotional events in a context-sensitive and goal-oriented manner is essential for adaptive functioning. In models of behavioral and emotion regulation, the lateral prefrontal cortex (LPFC) is postulated to maintain goal-relevant representations that promote cognitive control, an idea rarely tested with causal inference. Here, we altered mid-LPFC function in healthy individuals using a putatively inhibitory brain stimulation protocol (continuous theta burst; cTBS), followed by fMRI scanning. Participants performed the Affective Go/No-Go task, which requires goal-oriented action during affective processing. We targeted mid-LPFC (vs. a Control site) based on the individualized location of action-goal representations observed during the task. cTBS to mid-LPFC reduced action-goal representations in mid-LPFC and impaired goal-oriented action, particularly during processing of negative emotional cues. During negative-cue processing, cTBS to mid-LPFC reduced functional coupling between mid-LPFC and nodes of the default mode network, including frontopolar cortex—a region thought to modulate LPFC control signals according to internal states. Collectively, these results indicate that mid-LPFC goal-relevant representations play a causal role in governing context-sensitive cognitive control during emotional processing.
Elevated Na is a dynamic and reversible modulator of mitochondrial metabolism in the heart
Authors: Chung Yu Jin , Hoare Zoe , Baark Friedrich , Yu Chak Shun , Guo Jia , Fuller William , Southworth Richard , Katschinski Doerthe M. , Murphy Michael P. , Eykyn Thomas R. , Shattock Michael J. 
Published: May 2024

AbstractElevated intracellular sodium Nai adversely affects mitochondrial metabolism and is a common feature of heart failure. The reversibility of acute Na induced metabolic changes is evaluated in Langendorff perfused rat hearts using the Na/K ATPase inhibitor ouabain and the myosin-uncoupler para-aminoblebbistatin to maintain constant energetic demand. Elevated Nai decreases Gibb’s free energy of ATP hydrolysis, increases the TCA cycle intermediates succinate and fumarate, decreases ETC activity at Complexes I, II and III, and causes a redox shift of CoQ to CoQH2, which are all reversed on lowering Nai to baseline levels. Pseudo hypoxia and stabilization of HIF-1α is observed despite normal tissue oxygenation. Inhibition of mitochondrial Na/Ca-exchange with CGP-37517 or treatment with the mitochondrial ROS scavenger MitoQ prevents the metabolic alterations during Nai elevation. Elevated Nai plays a reversible role in the metabolic and functional changes and is a novel therapeutic target to correct metabolic dysfunction in heart failure.
Heuristics in risky decision-making relate to preferential representation of information
Authors: Russek Evan M. , Moran Rani , Liu Yunzhe , Dolan Raymond J. , Huys Quentin J. M. 
Published: May 2024

AbstractWhen making choices, individuals differ from one another, as well as from normativity, in how they weigh different types of information. One explanation for this relates to idiosyncratic preferences in what information individuals represent when evaluating choice options. Here, we test this explanation with a simple risky-decision making task, combined with magnetoencephalography (MEG). We examine the relationship between individual differences in behavioral markers of information weighting and neural representation of stimuli pertinent to incorporating that information. We find that the extent to which individuals (N = 19) behaviorally weight probability versus reward information is related to how preferentially they neurally represent stimuli most informative for making probability and reward comparisons. These results are further validated in an additional behavioral experiment (N = 88) that measures stimulus representation as the latency of perceptual detection following priming. Overall, the results suggest that differences in the information individuals consider during choice relate to their risk-taking tendencies.
Homozygous EPRS1 missense variant causing hypomyelinating leukodystrophy-15 alters variant-distal mRNA m6A site accessibility
Authors: Khan Debjit , Ramachandiran Iyappan , Vasu Kommireddy , China Arnab , Khan Krishnendu , Cumbo Fabio , Halawani Dalia , Terenzi Fulvia , Zin Isaac , Long Briana , Costain Gregory , Blaser Susan , Carnevale Amanda , Gogonea Valentin , Dutta Ranjan , Blankenberg Daniel , Yoon Grace , Fox Paul L. 
Published: May 2024

AbstractHypomyelinating leukodystrophy (HLD) is an autosomal recessive disorder characterized by defective central nervous system myelination. Exome sequencing of two siblings with severe cognitive and motor impairment and progressive hypomyelination characteristic of HLD revealed homozygosity for a missense single-nucleotide variant (SNV) in EPRS1 (c.4444 C > A; p.Pro1482Thr), encoding glutamyl-prolyl-tRNA synthetase, consistent with HLD15. Patient lymphoblastoid cell lines express markedly reduced EPRS1 protein due to dual defects in nuclear export and cytoplasmic translation of variant EPRS1 mRNA. Variant mRNA exhibits reduced METTL3 methyltransferase-mediated writing of N6-methyladenosine (m6A) and reduced reading by YTHDC1 and YTHDF1/3 required for efficient mRNA nuclear export and translation, respectively. In contrast to current models, the variant does not alter the sequence of m6A target sites, but instead reduces their accessibility for modification. The defect was rescued by antisense morpholinos predicted to expose m6A sites on target EPRS1 mRNA, or by m6A modification of the mRNA by METTL3-dCas13b, a targeted RNA methylation editor. Our bioinformatic analysis predicts widespread occurrence of SNVs associated with human health and disease that similarly alter accessibility of distal mRNA m6A sites. These results reveal a new RNA-dependent etiologic mechanism by which SNVs can influence gene expression and disease, consequently generating opportunities for personalized, RNA-based therapeutics targeting these disorders.
Concentration-induced spontaneous polymerization of protic ionic liquids for efficient in situ adhesion
Authors: Zhang Jun , Zhou Xuan , Hu Qinyu , Zhou Kaijian , Zhang Yan , Dong Shengyi , Zhao Gai , Zhang Shiguo 
Published: May 2024

AbstractThe advancement of contemporary adhesives is often limited by the balancing act between cohesion and interfacial adhesion strength. This study explores an approach to overcome this trade-off by utilizing the spontaneous polymerization of a protic ionic liquid-based monomer obtained through the neutralization of 2-acrylamide-2-methyl propane sulfonic acid and hydroxylamine. The initiator-free polymerization process is carried out through a gradual increase in monomer concentration in aqueous solutions caused by solvent evaporation upon heating, which results in the in-situ formation of a tough and thin adhesive layer with a highly entangled polymeric network and an intimate interface contact between the adhesive and substrate. The abundance of internal and external non-covalent interactions also contributes to both cohesion and interfacial adhesion. Consequently, the produced protic poly(ionic liquid)s exhibit considerable adhesion strength on a variety of substrates. This method also allows for the creation of advanced adhesive composites with electrical conductivity or visualized sensing functionality by incorporating commercially available fillers into the ionic liquid adhesive. This study provides a strategy for creating high-performance ionic liquid-based adhesives and highlights the importance of in-situ polymerization for constructing adhesive composites.
New insight into tuning magnetic phases of RMn6Sn6 kagome metals
Authors: Riberolles S. X. M. , Han Tianxiong , Slade Tyler J. , Wilde J. M. , Sapkota A. , Tian Wei , Zhang Qiang , Abernathy D. L. , Sanjeewa L. D. , Bud’ko S. L. , Canfield P. C. , McQueeney R. J. , Ueland B. G. 
Published: May 2024

AbstractPredicting magnetic ordering in kagome compounds offers the possibility of harnessing topological or flat-band physical properties through tuning of the magnetism. Here, we examine the magnetic interactions and phases of ErMn6Sn6 which belongs to a family of RMn6Sn6, R = Sc, Y, Gd–Lu, compounds with magnetic kagome Mn layers, triangular R layers, and signatures of topological properties. Using results from single-crystal neutron diffraction and mean-field analysis, we find that ErMn6Sn6 sits close to the critical boundary separating the spiral-magnetic and ferrimagnetic ordered states typical for non-magnetic versus magnetic R layers, respectively. Finding interlayer magnetic interactions and easy-plane Mn magnetic anisotropy consistent with other members of the family, we predict the existence of a number of temperature and field dependent collinear, noncollinear, and noncoplanar magnetic phases. We show that thermal fluctuations of the Er magnetic moment, which act to weaken the Mn-Er interlayer magnetic interaction and quench the Er magnetic anisotropy, dictate magnetic phase stability. Our results provide a starting point and outline a multitude of possibilities for studying the behavior of Dirac fermions in RMn6Sn6 compounds with control of the Mn spin orientation and real-space spin chirality.
A replicating RNA vaccine confers protection in a rhesus macaque model of Crimean-Congo hemorrhagic fever
Authors: Hawman David W. , Leventhal Shanna S. , Meade-White Kimberly , Khandhar Amit , Murray Justin , Lovaglio Jamie , Shaia Carl , Saturday Greg , Hinkley Troy , Erasmus Jesse , Feldmann Heinz 
Published: May 2024

AbstractCrimean-Congo hemorrhagic fever (CCHF) is a tick-borne febrile illness with a wide geographic distribution. In recent years the geographic range of Crimean-Congo hemorrhagic fever virus (CCHFV) and its tick vector have increased, placing an increasing number of people at risk of CCHFV infection. Currently, there are no widely available vaccines, and although the World Health Organization recommends ribavirin for treatment, its efficacy is unclear. Here we evaluate a promising replicating RNA vaccine in a rhesus macaque (Macaca mulatta) model of CCHF. This model provides an alternative to the established cynomolgus macaque model and recapitulates mild-to-moderate human disease. Rhesus macaques infected with CCHFV consistently exhibit viremia, detectable viral RNA in a multitude of tissues, and moderate pathology in the liver and spleen. We used this model to evaluate the immunogenicity and protective efficacy of a replicating RNA vaccine. Rhesus macaques vaccinated with RNAs expressing the CCHFV nucleoprotein and glycoprotein precursor developed robust non-neutralizing humoral immunity against the CCHFV nucleoprotein and had significant protection against the CCHFV challenge. Together, our data report a model of CCHF using rhesus macaques and demonstrate that our replicating RNA vaccine is immunogenic and protective in non-human primates after a prime-boost immunization.