bluuebunny/crossref_metadata_2025_split huggingface dataset:
Historical texts as a potential resource for plant-derived natural products against SARS-CoV-2 – the example of theReceptariumof Burkhard III von Hallwyl from 16th century Switzerland
Authors: Vahekeni Nina , Stehlin Jonas , Urmann Corinna , Wolfram Evelyn , Geissmann Yannick , Ruedin Yelena , Engler Olivier , Lardos Andreas 
Published: June 2024

AbstractIn the search for more effective prophylactic and possibly curative therapeutics against SARS-CoV-2, an historical-ethnobotanical approach was used to select plants described in theReceptariumof Burkhard III von Hallwyl (RBH), an influential recipe text from 16th century Switzerland. Ten species were identified based on specific historical uses presumably linked with the treatment of viral infections as well as inflammatory conditions. For each plant candidate, aqueous and hydroethanolic extracts have been produced. CellTiter-Glo® Luminescent Cell Viability Assay was used to assess antiviral activity against SARS-CoV-2 and the effect on cell viability of the extracts.Of the ten plant species tested, four displayed an antiviral activity ≥ 50% at 16.7 µg/ml with acceptable cell viability (> 75%):Sambucus nigraL. (leaves),Viola odorataL. (leaves),Geranium robertianumL. (arial parts) andArtemisia vulgarisL. (aerial parts). The crude extracts were partitioned in aqueous and organic fractions and further analyzed. The ethyl acetate fractions ofS. nigra, V. odorataandG. robertianumexpressed significant antiviral activity of nearly 100% at 5.6 µg/ml (P < 0.05). The most potent inhibitory activity was observed for the ethyl acetate fraction ofViola odorataL. (leaves) with 87% at 1.9 µg/ml (P < 0.0001). Alongside bioactivity analysis phytochemical fingerprints were made, with the aim to understand important substance classes contained. Further investigations are required to explore the active principles.Our study shows that an ethnopharmacological approach based on historical records of traditional use to select potential herbal candidates coupled with a rational screening process enables an efficient search for plant-derived natural products with antiviral activity against SARS-CoV-2.
Hydration conditions as a critical factor in antibiotic-mediated bacterial competition outcomes
Authors: Beizman-Magen Yana , Orevi Tomer , Kashtan Nadav 
Published: June 2024

AbstractAntibiotic secretion plays a pivotal role in bacterial interference competition, yet the impact of environmental hydration conditions on such competition is not well-understood. Here, we investigate how hydration conditions affect interference competition among bacteria, studying the interactions between the antibiotic-producingBacillus velezensisFZB42 and two bacterial strains susceptible to its antibiotics:Xanthomonas euvesicatoria85-10 andPseudomonas syringaeDC3000. Our results show that wet-dry cycles significantly modify the response of the susceptible bacteria to both the supernatant and cells of the antibiotic-producing bacteria, compared to constantly wet conditions. Notably,X. euvesicatoriashows increased protection against both the cells and supernatants ofB. velezensisunder wet-dry cycles, whileP. syringaecells become more susceptible under wet-dry cycles. In addition, we observed a reciprocal interaction betweenP. syringaeandB. velezensis, whereP. syringaeinhibitsB. velezensisunder wet conditions. Our findings highlight the important role of hydration conditions in shaping bacterial interference competition, providing valuable insights into microbial ecology of water-unsaturated surfaces, with implications for applications such as biological control of plant pathogens and mitigating antibiotic resistance.
A photo-switchable gold nanoformulation based on the dCas9 protein for spatiotemporal controlled gene editing activation in vivo
Authors: Konstantinidou Soultana , Rocco Mafalda A. , Nocilla Francesco , Debellis Doriana , d’Amora Marta , De Angelis Francesco , Gabellini Chiara , Fuso Francesco , Tantussi Francesco , Raffa Vittoria 
Published: June 2024

ABSTRACTGenome editing allows for the manipulation of genomic DNA for biotechnology and biomedical applications, but the specificity and control of the editing process remain a challenge. This study introduces a nano-switch for spatiotemporal control of the editing process. The basic module of the nano-switch (the monomer) is composed of a gold nanorod conjugated with the dead Cas9. Based on mathematical models, we established the design and the mechanism of action of the nano-switch. Briefly, when two monomers, guided by their respective guide RNAs, form a dimer onto the DNA and get irradiated with a Near-Infrared pulsed laser resonant at the plasmonic properties of the dimer, they generate a localized heat that triggers a thermal break onto the DNA. The nano-switch was generated, validated, and tested in zebrafish embryos at the 1-cell stage. Molecular analysis of irradiated embryos showed targeted DNA mutations, validating the efficacy of the nano-switch as a tool for conditional gene editing that integrates the if-when-where functions.
Comparison of Protein Extraction Methods and Data Analysis Strategies for Complete Metaproteomic Soil Analysis
Authors: Mikolitis Abigale S. , Mach Phillip M. , Kroeger Marie E. , McBride Ethan M. , Glaros Trevor G. 
Published: June 2024

AbstractConsiderable microbial diversity has been discovered in soil through genomic sequencing. Despite its role in biogeochemical cycling, relatively little is known about the proteomic diversity of the soil microbiome as most commercially available soil kits focus on DNA/RNA extractions. Consequently, a plethora of protein extraction techniques have been developed for soil but have yet to be integrated into simplified, modern sample preparation techniques such as the S-Trap™. Furthermore, classical data analysis strategies for soil metaproteomics rely on genomically-informed databases for peptide/protein identification. This assumes that DNA/RNA extracts adequately represent the soil proteome. Within this study, we systematically assess several extraction techniques, developing a data processing pipeline which is driven by both proteomics and genomics to fully characterize the soil microbiome. Both pipelines reveal remarkably complementary data, with ∼60% of the protein identifications coming from Proteomically-derived databases. Sodium dodecyl sulfate-based extractions proved to provide the most unique protein identifications (∼3000 proteins), and by combining both proteomic and genomic-based results, the total protein identifications increased approximately 2-fold for each extraction. Combining these complementary data pipelines with improved extraction techniques can allow for drastically improved proteomic results (12,307 unique protein identifications), even from minute (50 mg) sample volumes. These enhancements to previous workflows can better describe the microbial diversity within soil and provide a deeper functional understanding of the soil microbiome.
Dorso-ventral hippocampal neural assemblies are preferentially reactivated during NREM sleep following an aversive experience
Authors: Morici JF , Paiva-Lima I , Pronier É , Girardeau G 
Published: June 2024

AbstractSleep plays a crucial role in memory stabilization. In the dorsal hippocampus (dHPC), fast oscillations (sharp-wave ripples) during non-REM sleep consolidate spatial memories through place cell reactivation. The hippocampal formation is heterogeneous; the dHPC processes mostly contextual information, while the ventral part (vHPC) encodes valence and anxiety. We propose that dorsal- ventral communication during sleep is involved in the consolidation of emotional memories, potentially integrating or segregating contextual and valence information. To test this, we recorded the electrophysiological activity in the dHPC and vHPC in rats during reward and aversion-motivated exploration of a linear track and subsequent sleep. We detected dorsal and ventral SWRs during NREM sleep as well as neuronal assemblies during each exploration type and identified dorsal, ventral, and joint (dorso-ventral) assemblies. We found that dorso-ventral hippocampal coordination involves coordinated ripples and bursts of ripples. Aversive joint assemblies exhibited higher reactivation during non-REM sleep than the joint assemblies from the reward condition. Interestingly, joint aversive assemblies reactivated selectively during dorso-ventral coordinated ripples. Furthermore, their reactivation strength positively correlated with their strength of activation during the task. These results suggest the existence of dorsal-ventral hippocampal functional communication capable of sustaining the selective reactivation of experiences based on their emotional valence.
Innovation and firm characteristics: evidence from India’s company-level data
Authors: Chattopadhyay Sadhan Kumar , Nath Siddhartha , Sengupta Sreerupa , Joshi Shruti 
Published: June 2024

Purpose
This paper tries to explain variation in company-level innovation activities based on certain firm-level characteristics in India between 2010 and 2020.

Design/methodology/approach
Probit and Heckman’s two-step estimation based on panel data consisting of annual consolidated financial statements of 8,529 companies.

Findings
Firm-level innovation activities were associated with larger company size, lower age, higher access to digital assets and voluntary expenses on environmental sustainability and social responsibility.

Research limitations/implications
This paper is based on company-level financial statements and hence does not include aspects related to human capital, managerial capacities, participation in the global value chain and collaboration with other industries or academia.

Originality/value
This paper uses alternative measures of innovation such as promotional expenses and holding of intangible assets by companies in India alongside R&D expenditure, which is largely used in the past literature. The company characteristics also include emerging areas such as digitalisation, and spending on environmental sustainability and social responsibility, in addition to the conventional factors such as firm size, age and exposure to exports.
Linking ethical leadership to employee's prohibitive voice: the role of reflective moral attentiveness and leader identification
Authors: Nguyen Hau Van , Nguyen Long Le Hoang 
Published: June 2024

PurposeThis paper considers the importance of prohibitive voice in organizations' health. This study aims to explore the role of ethical leadership, reflective moral attentiveness and leader identification.Design/methodology/approachTwo waves of data collection were conducted, including 534 responses in T1 and 242 in T2. This research evaluated hypotheses using Partial Least Squares Structural Equation Modeling approach with Smart PLS 3.2.9.FindingsEthical leadership has a direct positive effect on employees' prohibitive voice. Furthermore, reflective moral attentiveness mediates the associations between ethical leadership and prohibitive voice. Finally, leader identification moderates only the relationship between ethical leadership and employees' moral behavior (prohibitive voice), not moral cognition (reflective moral attentiveness).Originality/valueThis paper provided insight into how and when ethical leaders could promote prohibitive voice behavior. Specifically, the authors explored the mediating role of reflective moral attentiveness and the moderating role of leader identification.
Underpinning risk dimensions of sponsored institutional R&D projects through cutting-edge fuzzy TOPSIS and fuzzy DEMATEL approaches
Authors: Kumar Sachin , Singh Vinay 
Published: June 2024

PurposeThis study aims to enhance the success of Sponsored Institutional R&D Projects (SIRPs) by investigating the influence of risk dimensions over four criteria Cost overrun, Time overrun, Schedule overrun, and Quality of projects. The study identifies risk dimensions and prioritizes and explores causal relationships to guide risk mitigation strategies during project execution.Design/methodology/approachThis study was conducted with qualitative data obtained through a systematic questionnaire from 10 domain experts associated with SIRPs. Through an extensive literature review, the study identifies the ten risk dimensions, Fuzzy TOPSIS was employed for prioritizing the explored risk dimensions and Fuzzy DEMATEL explored the qualitative causal relationships among these dimensions. The sensitivity analysis was conducted to validate the robustness of findings by adjusting the weightage assigned to experts.FindingsThe findings reveal financial risk as the highest ranked and business risk as the least influential among identified dimensions. The study also highlights management risk as the most significant, followed by business risk and sensitivity analysis concludes that the findings are robust, lacking significant judgment bias.Research limitations/implicationsThis study contributes to risk management strategies for SIRPs, to ensure timely completion within the allocated budget. The findings of the study can be implemented in other such SIRPs. The study also informs funding agencies' policies and project monitoring mechanisms and advancing project management practicesOriginality/valueIts originality lies in being the first to systematically identify and prioritize risk dimensions, adding significant value to SIRP management and academic discourse on project management and risk analysis.
Does board industry expertise foster ESG strategy? The mediating role of environmental innovation
Authors: della Corte Gaetano , Ricci Federica , Saggese Sara , Sarto Fabrizia 
Published: June 2024

Purpose The study aims to empirically examine the effect of board industry expertise on environmental, social and governance (ESG) strategy, and the mediating role of environmental innovation.Design/methodology/approach Using an unbalanced sample of 341 publicly traded Italian non-financial firms and data collected from multiple sources over the period 2017–2021, this study applies single-mediator models via ordinary least squares regressions.Findings Results indicate that directors’ industry expertise improves the corporate orientation toward sustainability strategy that is reflected in ESG objectives. This effect is partly mediated by a greater level of environmental innovation.Practical implications The article suggests regulators to promote eco-innovation-friendly investment initiatives due to their value in advancing corporate sustainability strategies.Originality/value The research fills a gap in the literature that has never explored the effect of board industry expertise on sustainability-related outcomes. Moreover, it advances the debate on the implications of board human capital by assessing its influence on ESG strategy and environmental innovation.
Insight from industry-early lessons learned about AI adoption in core procurement processes, directions for managers and researchers
Authors: van Hoek Remko 
Published: June 2024

Purpose
There is a growing body of conceptual work considering the potential of AI in supply chain and procurement, and there is great interest in AI among managers. But, according to a recent study, digital strategies for procurement are often missing or not satisfactory. Literature offers conflicting guidance on possible adoption areas for AI in core procurement processes. Given the need for better digital strategies for procurement and the need to further develop the understanding of adoption potential, the purpose of this paper is to explore actual adoption levels, experienced benefits, readiness levels and barriers to implementation in industry. This informs nuanced, not hyped, managerial consideration and identifies further research opportunities.

Design/methodology/approach
Leveraging items used in literature to study adoption of other technologies, the authors conduct the first empirical exploration of actual adoption levels of AI in procurement. The authors do so by collecting survey responses in three manager workshops, and the authors use the workshops to seek manager input in the interpretation of findings and the identification of implications for managers and researchers.

Findings
There appears to be less consideration given to AI in procurement than interest in the topic might imply. Adoption levels are generally low, implying that there is a lot of room for the development of consideration, use cases and possible pilots by managers and researchers. The authors find procurement benefits of AI adoption to be broader than costs and productivity alone, including visibility and innovation. But, readiness appears to be at relatively low levels with factors commonly considered in literature, such as executive support and willingness to invest, less relevant than less widely considered elements such as human sense making and supplier readiness.

Originality/value
This first empirical exploration moves past conceptualization and the study of potential adoption into the study of actual adoption levels in different procurement core processes. The authors expand the consideration of readiness by including additional items of human sense making as called for in literature. The authors also include and develop supplier readiness consideration, which is often missing from research. With the help of participating managers, the authors are able to develop a more comprehensive framework for the consideration of AI adoption. This can help bring nuance, not hype, to consideration and provides a rich portfolio of research items and constructs to further explore.
Enlightened Declarations: Ottoman and Russian Proclamations in the Ottoman‐Russian War of 1768–1774
Authors: Karabıçak Yusuf Ziya 
Published: June 2024

AbstractThis article analyses the Ottoman and Russian proclamations during the Ottoman‐Russian War of 1768–1774 to understand their similarities and differences in discourse and their intended audiences, with a special focus on the elites of the Ottoman Empire. The article shows that Ottoman diplomacy practiced Enlightenment diplomacy in the same way as its rivals, using the language of enlightened absolutism to win the hearts and minds of the Polish nobility. However, the war made diplomacy and its accompanying discourses an internal affair, and the imperial elite rejected the idea of fighting for Polish liberties. This had a lasting impact on how Ottoman power was framed and understood by both the imperial elite and its subjects. This was the main difference between the Ottoman and Russian empires; the former had already created an elite consensus based on shared European cultural signifiers that depended on the European Enlightenment, while the latter never did.
Cardio‐oncology and radiation oncology: How collaboration between cardiologists and oncologists can be realised in radiation oncology
Authors: Birch Samuel , Otton James 
Published: June 2024

SummaryIncreased survivorship, improvements in cancer treatments, and the potential for cardiac side effects from cancer treatments have resulted in increased collaboration between oncologists and cardiologists and the development of cardio‐oncology clinics. This collaboration is important given its role in ensuring greater patient satisfaction, aiding teams of clinicians in making complex treatment decision, and ensuring cardiac complications are diagnosed at an early stage. The particularities of implementing this collaboration in the field of radiation oncology and how this setting is different from other areas of cardio‐oncology have not been well detailed in the literature. This paper will discuss what is currently understood about the need for and role of cardio‐oncology and what a cardio‐oncology services involves, with a particular emphasis on patient and clinician needs in the field of radiation oncology. The literature and recent guidelines do advocate for a detailed baseline assessment of cancer patients undergoing radiotherapy, especially patients with treatment or patient risk factors that increase their risk of cancer‐therapy related cardiotoxicity. Advancements in cardiac imaging techniques will be discussed as these may help to diagnose cardiac side effects of certain cancer treatments, including radiotherapy, at an early stage. A multi‐disciplinary and collaborative approach is well received by patients and such an approach, guided by the aim of maintaining a patient's cancer treatment wherever possible, should be the cornerstone of cardio‐oncology clinics regardless of the patient's treatment regime.
Establishment and validation of prediction model for atherosclerotic cardiovascular disease in patients with hyperuricemia
Authors: Du Xingchen , Chen Fangfang , He Yisheng , Zou Hejian , Pan HaiFeng , Zhu Xiaoxia 
Published: June 2024

AbstractObjectiveTo construct a risk prediction model for atherosclerotic cardiovascular disease (ASCVD) in patients with hyperuricemia.MethodsData in this study were obtained from the National Health and Nutrition Examination Survey (NHANES) (2007–2010). Participants from Huashan Hospital were included as an external validation. Logistic regression analysis was used to explore the relevant factors of ASCVD in patients with hyperuricemia. The discriminability of the model was evaluated using the area under the curve (AUC) statistic of the receiver operating characteristic curve. Hosmer–Lemeshow test, correction curve and decision curve analysis (DCA) were used to evaluate the model.ResultsA total of 389 patients collected from the NHANES were included in the final analysis. Logistic regression analysis showed that age, creatinine (Cr), glucose (Glu), serum uric acid (SUA), and history of gout were predictive factors for ASCVD in hyperuricemia (HUA) patients. These predictive factors were used to construct a nomogram. And 157 patients from NHANES were in the internal validation group and 136 patients from Huashan Hospital were in the external validation group. The AUC values of the three groups were 0.943, 0.735, and 0.664. The p values of the Hosmer–Lemeshow test were .568, .600, and .763. The calibration curve showed consistency between the nomogram and the actual observed values. The DCA curve indicated that the model has good clinical practicality.ConclusionThis study constructed the ASCVD risk prediction model for HUA patients, which is beneficial for medical staff to detect high‐risk populations of ASCVD in the early stage.