bluuebunny/crossref_metadata_2025_split huggingface dataset:
Variety in vegetable and fruit consumption and risk of bladder cancer in the European Prospective Investigation into Cancer and Nutrition
Authors: Büchner Frederike L. , Bueno‐de‐Mesquita H. Bas , Ros Martine M. , Kampman Ellen , Egevad Lars , Overvad Kim , Tjønneland Anne , Roswall Nina , Clavel‐Chapelon Françoise , Boutron‐Ruault Marie‐Christine , Touillaud Marina , Kaaks Rudolf , Chang‐Claude Jenny , Boeing Heiner , Weikert Steffen , Trichopoulou Antonia , Naska Ada , Benetou Vicky , Palli Domenico , Sieri Sabina , Vineis Paolo , Tumino Rosario , Panico Salvatore , van Duijnhoven Fränzel J.B. , Peeters Petra H.M. , van Gils Carla H. , Lund Eiliv , Gram Inger T. , Sánchez Maria‐José , Jakszyn Paula , Larrañaga Nerea , Ardanaz Eva , Navarro Carmen , Rodríguez Laudina , Manjer Jonas , Ehrnström Roy , Hallmans Göran , Ljungberg Börje , Key Tim J. , Allen Naomi E. , Khaw Kay‐Tee , Wareham Nicholas , Slimani Nadia , Jenab Mazda , Boffetta Paolo , Kiemeney Lambertus A.L.M , Riboli Elio 
Published: August 2010

AbstractRecent research does not show an association between fruit and vegetable consumption and bladder cancer risk. None of these studies investigated variety in fruit and vegetable consumption, which may capture different aspects of consumption. We investigated whether a varied consumption of vegetables and fruits is associated with bladder cancer risk in the European Prospective Investigation into Cancer and Nutrition (EPIC) study. Detailed data on food consumption and complete follow‐up for cancer incidence were available for 452,185 participants, who were recruited from ten European countries. After a mean follow‐up of 8.7 years, 874 participants were diagnosed with bladder cancer. Diet diversity scores (DDSs) were used to quantify the variety in fruit and vegetable consumption. Multivariable Cox proportional hazard models were used to assess the effect of the DDSs on bladder cancer risk. There was no evidence of a statistically significant association between bladder cancer risk and any of the DDSs when these scores were considered as continuous covariates. However, the hazard ratio (HR) for the highest tertile of the DDS for combined fruit and vegetable consumption was marginally significant compared to the lowest (HR = 1.30, 95% confidence interval: 1.00–1.69, p‐trend = 0.05). In EPIC, there is no clear association between a varied fruit and vegetable consumption and bladder cancer risk. This finding provides further evidence for the absence of any strong association between fruit and vegetable consumption as measured by a food frequency questionnaire and bladder cancer risk.
Characterization of E2F3a function in HepG2 liver cancer cells
Authors: Li Wei , Ni Guo‐Xin , Zhang Ping , Zhang Zheng‐Xi , Li Wei , Wu Qiang 
Published: August 2010

AbstractE2F3a is a transcription factor that has been shown to be overexpressed in liver cancer tissues. To characterize the function of E2F3a in hepatocellular carcinoma (HCC), effects of ectopic overexpression of E2F3a on cell cycle, apoptosis, and gene expression of HepG2 cells were studied. E2F3a significantly enhances the apoptotic rate of HepG2 cells by 33% but only has minor effects on cell proliferation. By using microarray analyses, we identified 162 target genes (160 upregulated and 2 downregulated) of the E2F3a. Differential expression of 11 genes was further confirmed by real‐time PCR. Eight of these 11 genes, including XAF1, CEACAM1, STAT1, ATF3, TNFSF10, KLF6, CLDN1, and TAP1, were confirmed to be upregulated by more than twofold. Functional enrichments of differentially expressed genes retrieved 21 apoptosis‐related genes and 32 transcriptional regulation‐related genes. These results suggest that E2F3a induces apoptosis in HepG2 cells and plays important roles in regulating transcription. Finally, positive correlation was found between E2F3a and CEACAM1 mRNA levels in clinically well‐differentiated human HCC specimens. J. Cell. Biochem. 111: 1244–1251, 2010. © 2010 Wiley‐Liss, Inc.
Modulation of GEF‐H1 Induced Signaling by Heparanase in Brain Metastatic Melanoma Cells
Authors: Ridgway Lon D. , Wetzel Michael D. , Marchetti Dario 
Published: August 2010

AbstractMechanisms of brain metastatic melanoma (BMM) remain largely unknown. Understanding the modulation of signaling pathways that alter BMM cell invasion and metastasis is critical to develop new therapies for BMM. Heparanase has been widely implicated in cancer and is the dominant mammalian endoglycosidase which degrades heparan sulfate chains of proteoglycans (HSPG) including syndecans (SDCs). Recent findings also indicate that heparanase possesses non‐enzymatic functions in its latent form. We hypothesized that extracellular heparanase modulates BMM cell signaling by involving SDC1/4 carboxy terminal—associated proteins and downstream targets. We digested BMM cell surface HS with human recombinant active or latent heparanase to delineate their effects on cytoskeletal dynamics and cell invasiveness. We identified the small GTPase guanine nucleotide exchange factor‐H1 (GEF‐H1) as a new component of a SDC signaling complex that is differentially expressed in BMM cells compared to corresponding non‐metastatic counterparts. Second, knockdown of GEF‐H1, SDC1, or SDC4 decreased BMM cell invasiveness and GEF‐H1 modulated small GTPase activity of Rac1 and RhoA in conjunction with heparanase treatment. Third, both active and latent forms of heparanase affected Rac1 and RhoA activity; notably increasing RhoA activity. Both forms of heparanase were found to mediate the expression and subcellular localization of GEF‐H1, and treatment of BMM with latent heparanase modulated SDC1/4 gene expression. Finally, treatment with exogenous heparanase downregulated BMM cell invasion. These studies indicate the relevance of heparanase signaling pathways in BMM progression, and provide insights into the molecular mechanisms regulating HSPG signaling in response to exogenous heparanase. J. Cell. Biochem. 111: 1299–1309, 2010. © 2010 Wiley‐Liss, Inc.
SIRT1 modulates high‐mobility group box 1‐induced osteoclastogenic cytokines in human periodontal ligament cells
Authors: Kim Young‐Suk , Lee Young‐Man , Park Jae‐Sang , Lee Suk‐Keun , Kim Eun‐Cheol 
Published: August 2010

AbstractBone resorptive cytokines contribute to bone loss in periodontal disease. However, the involvement of SIRT1 in high‐mobility group box 1 (HMGB1)‐induced osteoclastic cytokine production remains unknown. The aim of this study was to investigate the role of SIRT1 in the responses of human periodontal ligament cells to HMGB1 and to identify the underlying mechanisms. The effect of HMGB1 on osteoclastic cytokine expression and secretion, and the regulatory mechanisms involved were studied by ELISA, reverse transcription‐polymerase chain reaction, and Western blot analysis. HMGB1 upregulated the mRNA expression levels of the osteoclastic cytokines tumor necrosis factor (TNF)‐α, interleukin (IL)‐1β, IL‐6, IL‐11, and IL‐17. In addition, HMGB1 upregulated RANKL mRNA expression, and SIRT1 mRNA and protein expression. The upregulation of these cytokines by HMGB1 was attenuated by pretreatment with inhibitors of p38 mitogen‐activated protein kinase and NF‐κB, as well as neutralizing antibodies against Toll‐like receptors 2 and 4. Inhibition of SIRT1 by sirtinol or SIRT1 siRNA blocked the HMGB1‐stimulated expression of RANKL and cytokines. These results suggest that the inhibition of SIRT1 may attenuate HMGB1‐mediated periodontal bone resorption through the modulation of osteoclastogenic cytokine levels in human periodontal ligament cells. J. Cell. Biochem. 111: 1310–1320, 2010. © 2010 Wiley‐Liss, Inc.
Intramolecular interactions of L‐phenylalanine: Valence ionization spectra and orbital momentum distributions of its fragment molecules
Authors: Ganesan Aravindhan , Wang Feng , Falzon Chantal 
Published: August 2010

AbstractIntramolecular interactions between fragments of L‐phenylalanine, i.e., phenyl and alaninyl, have been investigated using dual space analysis (DSA) quantum mechanically. Valence space photoelectron spectra (PES), orbital energy topology and correlation diagram, as well as orbital momentum distributions (MDs) of L‐phenylalanine, benzene and L‐alanine are studied using density functional theory methods. While fully resolved experimental PES of L‐phenylalanine is not yet available, our simulated PES reproduces major features of the experimental measurement. For benzene, the simulated orbital MDs for 1e1g and 1a2u orbitals also agree well with those measured using electron momentum spectra. Our theoretical models are then applied to reveal intramolecular interactions of the species on an orbital base, using DSA. Valence orbitals of L‐phenylalanine can be essentially deduced into contributions from its fragments such as phenyl and alaninyl as well as their interactions. The fragment orbitals inherit properties of their parent species in energy and shape (ie., MDs). Phenylalanine orbitals show strong bonding in the energy range of 14‐20 eV, rather than outside of this region. This study presents a competent orbital based fragments‐in‐molecules picture in the valence space, which supports the fragment molecular orbital picture and building block principle in valence space. The optimized structures of the molecules are represented using the recently developed interactive 3D‐PDF technique. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2011
Synthesis of novel carbazolyl‐oxazolone derivatives and their spectroscopic properties
Authors: Gündoğdu Cevher , Topkaya Derya , Öztürk Gülsiye , Alp Serap , Ergün Yavuz 
Published: August 2010

Abstract
magnified image Novel carbazolyl‐oxazolones, 3a, 3b, 3c, 3d, were synthesized with 3‐[N‐(2‐hydroxycarbonylmethyl)‐carboxamide]‐4‐methyl‐9H‐carbazole and several aryl aldehydes for the first time. Photo physical characterization of synthesized 2‐carbazolyl‐4‐arylidene‐5‐oxazolones (3a, 3b, 3c, 3d) in dichloromethane, chloroform, and toluene was performed. J. Heterocyclic Chem., (2010).
Up‐regulation of erythropoietin receptor by nitric oxide mediates hypoxia preconditioning
Authors: Chen Zhi‐Yong , Wang Li , Asavaritkrai Pundit , Noguchi Constance Tom 
Published: August 2010

AbstractErythropoietin (Epo), known to stimulate erythroid progenitor cell survival, proliferation, and differentiation, has been shown to be neuroprotective against brain ischemia in animal models. Both Epo and Epo receptor (EpoR) are expressed in the brain and are up‐regulated by hypoxia. Brain Epo signaling can stimulate neural cell survival and prevent neuron apoptosis. Neurons from EpoR null mice exhibit marked increased sensitivity to hypoxia. In endothelial cells, Epo has been shown to stimulate nitric oxide (NO) production, particularly at low pO2. We found here that the EpoR expression on neural cells and Epo's neuroprotective effect were regulated by NO. Hypoxia increased NO production as well as EpoR expression, and inhibition of NOS activity reduced the proportion of EpoR‐expressing neurons induced at low pO2. Conversely, addition of NO donor to cultures grown under normoxia induced EpoR. Similarly, NO donor increased EpoR promoter activity in a reporter gene assay, suggesting that NO regulates EpoR at the transcription level. Preincubation of neurons with NO results in induction of EpoR, which gives rise to protection against hypoxia even in the absence of exogenous Epo, although at high concentration NO is toxic. These data provide evidence of a role for NO in Epo activity in brain and suggest links between NO production, EpoR expression, and Epo signaling in neuroprotection. Published 2010 Wiley‐Liss, Inc.
L10p and P158DEL DJ‐1 mutations cause protein instability, aggregation, and dimerization impairments
Authors: Ramsey Chenere P. , Giasson Benoit I. 
Published: August 2010

AbstractA variety of mutations in the gene encoding DJ‐1 protein cause autosomal recessive early‐onset parkinsonism. Recently, a novel pathogenic homozygous DJ‐1 missense mutation resulting in the L10P amino acid substitution was reported. In a separate study, a novel homozygous mutation resulting in the deletion of DJ‐1 residue P158 was also reported to be causative of disease. The specific effects of the novel L10P and P158DEL mutations on protein function have not been studied. Here, L10P and P158DEL DJ‐1 proteins were assessed for protein stability, dimerization, solubility, subcellular localization, and protective function in comparison with WT and the L166P DJ‐1 pathogenic variant. It was discovered that, compared with WT protein, L10P, L166P, and P158DEL DJ‐1 variants exhibited dramatically reduced protein stabilities. Degradation of each of the pathogenic mutants appeared to be mediated in part by the proteasome. Interestingly, unlike L166P DJ‐1, the L10P and P158DEL DJ‐1 variants retained the ability to dimerize with WT DJ‐1 protein; however, neither of these mutants was able to form homodimers. Additionally, the L10P, L166P, and P158DEL DJ‐1 variants exhibited altered profiles on size‐exclusion chromatography and demonstrated reduced solubilities in comparison with WT protein, and the latter aberration could be exacerbated in the presence of MG‐132. Furthermore, cells stably expressing L10P DJ‐1 were more vulnerable to treatments with proteasome inhibitors, suggesting that L10P DJ‐1 may be toxic to cells under conditions of proteasome stress. Taken together, these findings suggest that diverse aberrant mechanisms, including alterations in protein stability and protein folding, are associated with the pathogenicity of the L10P and P158DEL DJ‐1 variants. © 2010 Wiley‐Liss, Inc.
Mutation of iceA in Helicobacter pylori compromised IL‐8 induction from human gastric epithelial cells
Authors: Ma Yi Jun , Duan Guang Cai , Zhang Rong Guang , Fan Qing Tang , Zhang Wei Dong 
Published: August 2010

AbstractIceA of Helicobacter pylori (H. pylori) has been suggested as a virulence factor for the bacteria, but its pathogenic role remains unelucidated. Here, we examined the effect of iceA mutation on the secretion of IL‐8 by human gastric epithelial cells. We also investigated whether the changes in IL‐8 production caused by iceA mutation were associated with impaired adherence of H. pylori to the epithelial cells or with impaired apoptosis of these cells. The iceA mutant strain was constructed from wildtype H. pylori strain by insertional mutagenesis of iceA. The human gastric epithelial cells SGC7901 were infected with wildtype or mutant H. pylori for appropriate lengths of time. The adherence of the bacteria to the epithelial cells was examined by fluorescent microscopy using an anti‐H. pylori antibody and flow cytometry. The apoptosis of the epithelial cells was studied by annexin‐V staining and flow cytometry. The production of IL‐8 by SGC 7901 cells was determined by ELISA. We found that iceA mutation was associated with significantly impaired production of IL‐8 from the epithelial cells, which was not due to impaired adherence by the bacteria to the epithelial cells as wildtype and mutant H. pylori exhibited similar levels of binding to the epithelial cells. Furthermore, inactivation of iceA did not affect the apoptotic cell death of SGC7901. Our findings indicate that iceA may contribute to the pathogenicity of H. pylori by modulating the production of IL‐8 by host epithelial cells. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Optimization of laccase production by Pleurotus ostreatus IMI 395545 using the Taguchi DOE methodology
Authors: Periasamy Rathinasamy , Palvannan Thayumanavan 
Published: August 2010

AbstractProduction of laccase using a submerged culture of Pleurotus orstreatus IMI 395545 was optimized by the Taguchi orthogonal array (OA) design of experiments (DOE) methodology. This approach facilitates the study of the interactions of a large number of variables spanned by factors and their settings, with a small number of experiments, leading to considerable savings in time and cost for process optimization. This methodology optimizes the number of impact factors and enables to calculate their interaction in the production of industrial enzymes. Eight factors, viz. glucose, yeast extract, malt extract, inoculum, mineral solution, inducer (1 mM CuSO4) and amino acid (l‐asparagine) at three levels and pH at two levels, with an OA layout of L18 (21 × 37) were selected for the proposed experimental design. The laccase yield obtained from the 18 sets of fermentation experiments performed with the selected factors and levels was further processed with Qualitek‐4 software. The optimized conditions shared an enhanced laccase expression of 86.8% (from 485.0 to 906.3 U). The combination of factors was further validated for laccase production and reactive blue 221 decolorization. The results revealed an enhanced laccase yield of 32.6% and dye decolorization up to 84.6%. This methodology allows the complete evaluation of main and interaction factors. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Do adolescents know when they need help in the aftermath of war?
Authors: Schiff Miriam , Pat‐Horenczyk Ruth , Benbenishty Rami , Brom Danny , Baum Naomi , Astor Ron Avi 
Published: August 2010

AbstractThis study examined Israeli Arab and Jewish students' reports on needing help, a year after the Second Lebanon War and whether students' requests for support were associated with posttraumatic distress. The representative sample included 1,800 Jewish and 2,351 Arab students, grades 7–11. The questionnaires included items regarding (a) exposure to wartime events and other negative life events, (b) measures of posttraumatic stress disorder, and (c) needing help. The results showed that about 30% of the students reported needing help from any source (e.g., parents, peers) in the aftermath of the war. Arab students were more likely to report needing help than Jewish students. The students who reported needing help experienced higher levels of posttraumatic symptoms.
The Joseph H. Ogura lecture: Teaching and learning—then and now
Authors: Fried Marvin P. 
Published: August 2010

AbstractEducation, the process of teaching and learning, has remained a passion of those in academic medicine. The context has changed over time, but the skills required are universal. The goal of these efforts is training the finest otolaryngologists who deliver the best care to their patients and build the future or our specialty. Laryngoscope, 2011
Analysis of skeletal mandibular abnormalities associated with cervicofacial lymphatic malformations
Authors: O Teresa M. , Kwak Roy , E. Portnof Jason , Berke Daryl M. , Lipari Brian , Waner Milton 
Published: August 2010

AbstractObjectives/HypothesisMany children with cervicofacial lymphatic malformations have facial skeletal abnormalities. This study qualitatively and quantitatively evaluated these bony mandibular abnormalities.Study DesignRetrospective chart review.MethodsPatients with craniofacial lymphatic malformations presenting to a vascular anomalies center during a 2‐year period were included. An age‐matched control population was found on the Picture Archiving and Communication System database. Three‐dimensional reconstructions were created from computed tomography and magnetic resonance imaging. Qualitative observations and quantitative measurements were taken of the gonial angle, mandibular anterior dentoalveolar height, and anterior condylar displacement.ResultsThere were 23 controls. A total of 21 patients with “beard” distribution malformations were studied; 10 had unilateral and 11 had bilateral disease.Qualitatively, a few patterns emerged: outward ramal flaring, anterior displacement of the mandible, relative ipsilateral facial “hypertrophy,” and anterior positioning of the maxilla and orbit. The open‐bite deformity was a common finding leading to malocclusion and oral incompetence. Quantitatively, in 67 nondiseased sides, the average gonial angle was 131 degrees (standard deviation [SD] = 6.8), whereas in 32 diseased sides, the average angle was 152 degrees (SD = 14.0, P < 3.8E‐09). With half‐beard malformations, the diseased side averaged 153.9 and the nondiseased side averaged 140.8 (P = .008). The average condylar displacement was 8.5 mm versus 5.9 mm (diseased vs. nondiseased), and the average mandibular dentoalveolar height to face ratio was 0.37 (control = 0.34).ConclusionsCervicofacial lymphatic malformations in the “beard” distribution are associated with significant bony abnormalities leading to both functional and aesthetic sequelae. Our study is the first quantitative analysis of these changes. This is the first step in planning for dentofacial orthopedics, orthodontics, and orthognathic surgery. Laryngoscope, 2011
Wet‐Chemical Deposition of Functional Coatings for Bone Implantology
Authors: Nijhuis Arnold W. G. , Leeuwenburgh Sander C. G. , Jansen John A. 
Published: August 2010

AbstractSince the early 1980s, research on the modification of bone implant surfaces by applying coatings has mainly focused on the application of inorganic calcium phosphate (CaP) coatings using physical deposition techniques. Organic components of the extracellular bone matrix, on the other hand, play an essential role in the process of bone healing, but cannot be applied using these physical techniques. Therefore, a recent trend in biomaterials research involves development of novel wet‐chemical deposition techniques for both inorganic and organic coatings. This study reviews the major wet‐chemical coating techniques that are used for the deposition of inorganic CaP coatings and organic biomolecules coatings.magnified image
Efficient Molecular Weight Control with Trialkylaluminum in Ethylene/Norbornene Copolymerization by [Ph2C(Flu)(3‐MeCp)]ZrCl2/Methylaluminoxane Catalyst
Authors: Tada Tomoyuki , Cai Zhengguo , Nakayama Yuushou , Shiono Takeshi 
Published: August 2010

AbstractEthylene/norbornene copolymerizations were conducted with [Ph2C(Flu)(3‐RCp)]ZrCl2 [R: Me (1) or Me3Si (2)], which give alternating EN copolymers. The activity of 1 in the absence of R3Al was approximately twice that of 2 and increased further upon addition of R3Al. Et3Al increased the activity most effectively, and the $\overline {M} _{{\rm n}} $ of the produced polymer decreased from 100 000 to 25 000 g · mol−1. On the other hand, the $\overline {M} _{{\rm n}} $ value increased upon addition of iBu3Al from 100 000 to 209 000 g · mol−1 accompanied by an ≈2.5‐fold increase of activity. Consequently, 1 was found to be one of the most promising complexes for the synthesis of alternating EN copolymers, of which $\overline {M} _{{\rm n}} $ value was efficiently controlled by the kind and the amount of R3Al added.magnified image
Die Chemie des Heliums
Authors: Cremer Dieter 
Published: August 2010

AbstractDie Quantenchemie hat sich während der beiden letzten Jahrzehnte zu einem der wichtigsten Teilgebiete der Theoretischen Chemie entwickelt.1) Ursachen dafür sind die ständige Verbesserung quantenchemischer Methoden und vor allem die atemberaubende Weiterentwicklung von Hochleistungscomputern.
Kunststoffe bleiben weiterhin die Hauptträger des Chemie‐Wachstums
Authors: Nachtsheim Hubert 
Published: August 2010

AbstractZufriedenheit und Optimismus beherrschten die Stimmung beim Pressegespräch des Verbandes Kunststofferzeugende Industrie (VKE) im „Hessischen Hoff”︁ zu Frankfurt. Der Vorsitzende, Bayer‐Vorstandsmitglied Dr. Helmut Piechota, kam in seinem Statement zur Sache: Die deutschen Kunststofferzeuger haben im Rahmen der guten Konjunktur 1988 ein ausgezeichnetes Ergebnis erwirtschaften können. Piechota: “An den erfreulich gestiegenen Umsätzen und Ergebnissen der deutschen Chemieunternehmen hatten Kunststoffe einen erheblichen Anteil. Sie waren eigentlich die wichtigen Träger des Wachstums.”