bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fabrication of a Thermoresponsive Biohybrid Double Hydrophilic Block Copolymer by a Cofactor Reconstitution Approach
Authors: Wan Xuejuan , Liu Shiyong 
Published: October 2010

AbstractThe fabrication of a thermoresponsive biohybrid double hydrophilic block copolymer (DHBC) by a cofactor reconstitution approach is reported. Poly(N‐isopropylacrylamide) (PNIPAM) bearing a porphyrin moiety at the chain terminal, PPIXZn‐PNIPAM, is synthesized by the combination of ATRP and a click reaction. The subsequent cofactor reconstitution process between apomyoglobin and PPIXZn‐PNIPAM affords well‐defined myoglobin‐b‐PNIPAM protein–polymer bioconjugates. Behaving as typical responsive DHBCs, the obtained myoglobin‐b‐PNIPAM biohybrid diblock copolymer exhibits thermo‐induced aggregation behavior in aqueous solution as a result of the presence of the thermoresponsive PNIPAM block, as revealed by temperature‐dependent transmittance, dynamic laser light scattering measurements, transmission electron microscopy, and scanning electron microscopy. This work represents the first report of the preparation of responsive biohybrid DHBCs by the cofactor reconstitution process.magnified image
Heteronuclear chemical shift correlation and J‐resolved MAS NMR spectroscopy of lipid membranes
Authors: Zorin Vadim , Ciesielski Filip , Griffin David C. , Rittig Michael , Bonev Boyan B. 
Published: October 2010

AbstractDirect observation of J‐couplings remains a challenge in high‐resolution solid‐state NMR. In some cases, it is possible to use Lee–Goldburg (LG) homonuclear decoupling during rare spin observation in MAS NMR correlation spectroscopy of lipid membranes to obtain J‐resolved spectra in the direct dimension. In one simple implementation, a wide line separation‐type 13C‐1H HETCOR can provide high‐resolution 1H/13C spectra, which are J‐resolved in both dimensions. Coupling constants, 1JHC, obtained from 1H doublets, can be compared with scaled 1JθCH‐values obtained from the 13C multiplets to assess the LG efficiency and scaling factor. The use of homonuclear decoupling during proton evolution, LG‐HETCOR‐LG, can provide J‐values, at least in the rare spin dimension, and allows measurements in less mobile membrane environments. The LG‐decoupled spectroscopic approach is demonstrated on pure dioleoylphosphatidylcholine (DOPC) membranes and used to investigate lipid mixtures of DOPC/cholesterol and DOPC/cholesterol/sphingomyelin. Copyright © 2010 John Wiley & Sons, Ltd.
Iopamidol as a responsive MRI‐chemical exchange saturation transfer contrast agent for pH mapping of kidneys: In vivo studies in mice at 7 T
Authors: Longo Dario Livio , Dastrù Walter , Digilio Giuseppe , Keupp Jochen , Langereis Sander , Lanzardo Stefania , Prestigio Simone , Steinbach Oliver , Terreno Enzo , Uggeri Fulvio , Aime Silvio 
Published: October 2010

AbstractIopamidol (Isovue®—Bracco Diagnostic Inc.) is a clinically approved X‐Ray contrast agent used in the last 30 years for a wide variety of diagnostic applications with a very good clinical acceptance. Iopamidol contains two types of amide functionalities that can be exploited for the generation of chemical exchange saturation transfer effect. The exchange rate of the two amide proton pools is markedly pH‐dependent. Thus, a ratiometric method for pH assessment has been set‐up based on the comparison of the saturation transfer effects induced by selective irradiation of the two resonances. This ratiometric approach allows to rule out the concentration effect of the contrast agent and provides accurate pH measurements in the 5.5–7.4 range. Upon injection of Iopamidol into healthy mice, it has been possible to acquire pH maps of kidney regions. Furthermore, it has been also shown that the proposed method is able to report about pH‐changes induced in control mice fed with acidified or basified water for a period of a week before image acquisition. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.
Hormone Changes Affecting Energy Homeostasis after Metabolic Surgery
Authors: Harvey E. John , Arroyo Kervin , Korner Judith , Inabnet William B. 
Published: October 2010

AbstractWorldwide, there is an epidemic of obesity and its associated diseases. The past decade of research has brought about a paradigm shift in our understanding of both the mechanisms underlying energy homeostasis and the multiple factors contributing to the pathophysiology of obesity. Metabolic surgery is currently far more effective than diet and exercise or pharmacotherapy in achieving durable weight loss. Moreover, the remarkable results of surgery in achieving a rapid remission of type 2 diabetes mellitus has sparked tremendous excitement and research into the mechanisms through which metabolic surgery has its dramatic effect. As opposed to the traditional understanding of “restriction” and “malabsorption,” current evidence suggests that metabolic surgery alters the expression of multiple hormones that affect both short‐term and long‐term signals of energy balance. We review the hormonal changes following the most common types of metabolic operations currently being performed. The profile of hormonal changes provides a guide to tailor the choice of operation for each individual patient toward achieving the desired metabolic result. In the future, individualized metabolic surgery alone or modulated by targeted pharmacological therapy may achieve the most reliable and effective results with the highest safety and lowest side effect profile. Mt Sinai J Med 77:446–465, © 2010 Mount Sinai School of Medicine
Plasma proteome analysis in diet‐induced obesity‐prone and obesity‐resistant rats
Authors: Choi Jung‐Won , Wang Xia , Joo Jeong In , Kim Dong Hyun , Oh Tae Seok , Choi Duk Kwon , Yun Jong Won 
Published: October 2010

AbstractOne of the major issues in the field of obesity is why some humans become obese and others resist development of obesity when exposed to high‐calorie diets. Despite the same genetic background, namely obesity‐prone (OP) and ‐resistant (OR) rats, differing responses have been demonstrated in a high fat diet‐induced rodent model. The aim of the present study was to discover novel obesity‐related biomarkers for susceptibility and/or resistance to obesity by proteomic analysis of OP and OR rat plasma. After feeding of high fat diet, OP rats gained approximately 25% more body weight than OR rats and were used for proteomic analysis using 2‐DE combined with MALDI‐TOF‐MS. We categorized identified proteins into three groups by analysis of both average spot density in each group and individual spot density of six rats as a function of body weight. Consequently, category (1) included inter‐α‐inhibitor H4 heavy chain and fetuin B precursor, which can be used as novel plasma biomarkers for risk of obesity. Nine proteins of category (2) and (3) can also be plausible plasma markers in the study of obesity. This proteomic study is an important advancement over the previous steps needed for identification of OP and OR rats.
Effect of the Rho kinase inhibitor Y‐27632 on the proteome of hearts with ischemia–reperfusion injury
Authors: Cadete Virgilio J. J. , Sawicka Jolanta , Polewicz Dorota , Doroszko Adrian , Wozniak Mieczyslaw , Sawicki Grzegorz 
Published: October 2010

AbstractGrowing attention has been given to the role of the Rho kinase pathway in the development of heart disease and ischemia/reperfusion (I/R) injury. Y‐27632 is a Rho kinase inhibitor demonstrated to protect against I/R injury, but the exact mechanism by which it does so remains to be elucidated. The goal of this project was to determine new targets by which Y‐27632 can protect the heart against I/R injury. Isolated rat hearts were perfused under aerobic conditions or subjected to I/R in the presence or absence of Y‐27632. Administration of Y‐27632 (1 μM) before ischemia and during the first 10 min of reperfusion resulted in complete recovery of cardiac function. 2‐D electrophoresis followed by MS identified four proteins whose levels were affected by Y‐27632 treatment. Lactate dehydrogenase and glyceraldehyde‐3‐phosphate dehydrogenase were significantly increased in the Y‐27632 treated group, while creatine kinase was normalized to control levels. In addition, we found increased level of two different molecular fragments of ATP synthase, which were normalized by Y‐27632. This increase suggests that during ischemia ATP synthase is subjected to degradation. The changes in metabolic enzymes' levels and their regulation by Y‐27632 suggest that the cardioprotective effect of Y‐27632 involves increased energy production.
Mass spectrometry characterization of 3‐OH butyrated β‐cyclodextrin
Authors: Peptu Cristian , Nicolescu Alina , Peptu Catalina A. , Harabagiu Valeria , Simionescu Bogdan C. , Kowalczuk Marek 
Published: October 2010

AbstractOligo(3‐OH butyrate)‐β‐cyclodextrin esters (PHB‐CD) were obtained through ring opening of β‐butyrolactone (β‐BL) in the presence of β‐cyclodextrin (CD) and (‐)‐sparteine (SP) as nucleophilic activator. The resulted reaction mixture was first separated in two fractions and then investigated through a deep mass spectrometry (MS) study performed on a liquid chromatography‐electrospray ionization‐quadrupole time of flight (LC‐ESI‐QTOF) instrument. LC MS and tandem MS structural assignment of the reaction products was completed by NMR. The performed analysis revealed that poly(3‐OH butyrate) homopolymers (PHB) are formed together with the PHB‐CD products. Secondary reactions resulting in the formation of crotonates were also proved to occur. A comparison between MS and NMR results demonstrated that more than one PHB oligomer is attached to the CD in the PHB‐CD product. The tandem MS fragmentation studies validated the proposed structure of CD derivatives. © 2010 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2010
Rapid exploration of the folding topology of helical membrane proteins using paramagnetic perturbation
Authors: Yeo Kwon Joo , Kim Hye‐Yeon , Kim Young Pil , Hwang Eunha , Kim Myung Hee , Cheong Chaejoon , Choe Senyon , Jeon Young Ho 
Published: October 2010

AbstractAn understanding of the folding states of α‐helical membrane proteins in detergent systems is important for functional and structural studies of these proteins. Here, we present a rapid and simple method for identification of the folding topology and assembly of transmembrane helices using paramagnetic perturbation in nuclear magnetic resonance spectroscopy. By monitoring the perturbation of signals from glycine residues located at specific sites, the folding topology and the assembly of transmembrane helices of membrane proteins were easily identified without time‐consuming backbone assignment. This method is validated with Mistic (membrane‐integrating sequence for translation of integral membrane protein constructs) of known structure as a reference protein. The folding topologies of two bacterial histidine kinase membrane proteins (SCO3062 and YbdK) were investigated by this method in dodecyl phosphocholine (DPC) micelles. Combing with analytical ultracentrifugation, we identified that the transmembrane domain of YbdK is present as a parallel dimer in DPC micelle. In contrast, the interaction of transmembrane domain of SCO3062 is not maintained in DPC micelle due to disruption of native structure of the periplasmic domain by DPC micelle.
The anterior gradient 2 (AGR2) gene is overexpressed in prostate cancer and may be useful as a urine sediment marker for prostate cancer detection
Authors: Bu Huajie , Bormann Stefanie , Schäfer Georg , Horninger Wolfgang , Massoner Petra , Neeb Antje , Lakshmanan Vinoth‐Kumar , Maddalo Danilo , Nestl Andrea , Sültmann Holger , Cato Andrew C.B. , Klocker Helmut 
Published: October 2010

AbstractBACKGROUNDAGR2 is a member of the endoplasmatic reticulum protein disulphide isomerase gene family implicated in tumor metastasis. Its expression pattern, function, and utility as a marker remains to be further investigated.METHODSUsing real‐time RT‐PCR and immunohistochemistry, changes of expression in different tumor stages were explored in microdissected tumor samples. AGR2 transcript level in urine sediments was scrutinized for suitability as a tumor marker. AGR2 androgen regulation and function were analyzed in cellular prostate cancer models.RESULTSAGR2 is highly expressed in prostate cancer compared to benign tissue in particular also in low‐grade tumors and PIN lesions. AGR2 transcripts were detected in urine sediments of patients undergoing prostate biopsy with significantly higher levels in tumor patients. The urine AGR2/PSA transcript ratio allowed much better discrimination between cancer and benign patients than serum total PSA or %freePSA. Prostate tumor cells express and secrete variable amounts of AGR2 protein, the highest level was found in PC3 cells. In androgen receptor‐positive cell lines AGR2 is upregulated by androgens. Increased expression enhanced the migratory and invasive potential but decreased growth and proliferation in vitro and in vivo.CONCLUSIONAGR2 enhances the invasion phenotype of prostate cancer cells while at the same time attenuating cell‐cycle progression. This function, its expression pattern and the increased level of AGR transcripts in urine sediments of prostate cancer patients call for further exploration as a prostate cancer marker and a modulator of tumor growth and invasion. Prostate 71:575–587, 2011. © 2010 Wiley‐Liss, Inc.
Nanoporous materials: Porous Graphene as an Atmospheric Nanofilter (Small 20/2010)
Authors: Blankenburg Stephan , Bieri Marco , Fasel Roman , Müllen Klaus , Pignedoli Carlo A. , Passerone Daniele 
Published: October 2010

AbstractThe fabrication of nanoscale membranes exhibiting high selectivity is an emerging field of research. The possibility to use bottom‐up approaches to fabricate a filter with porous graphene and analyze its functionality with first principle calculations is investigated. Here, the porous network is produced by self‐assembly of the hexaiodo‐substituted macrocycle cyclohexa‐m‐phenylene (CHP). The resulting porous network exhibits an extremely high selectivity in favor of H2 and He among other atmospheric gases, such as Ne, O2, N2, CO, CO2, NH3, and Ar. The presented membrane is superior to traditional filters using polymers or silica and could have great potential for further technological applications such as gas sensors or fuel cells.
Irradiation Enhances the Tumor Tropism and Therapeutic Potential of Tumor Necrosis Factor-Related Apoptosis-Inducing Ligand-Secreting Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells in Glioma Therapy
Authors: Kim Seong Muk , Oh Ji Hyeon , Park Soon A , Ryu Chung Heon , Lim Jung Yeon , Kim Dal-Soo , Chang Jong Wook , Oh Wonil , Jeun Sin-Soo 
Published: October 2010

Abstract
Irradiation is a standard therapy for gliomas and many other cancers. Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is one of the most promising candidates for cancer gene therapy. Here, we show that tumor irradiation enhances the tumor tropism of human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) and the therapeutic effect of TRAIL delivered by UCB-MSCs. The sequential treatment with irradiation followed by TRAIL-secreting UCB-MSCs (MSC-TRAIL) synergistically enhanced apoptosis in either TRAIL-sensitive or TRAIL-resistant glioma cells by upregulating the death receptor 5 and by inducing caspase activation. Migration assays showed greater MSC migration toward irradiated glioma cells and the tumor site in glioma-bearing mice compared with unirradiated tumors. Irradiated glioma cells had increased expression of interleukin-8 (IL-8), which leads to the upregulation of the IL-8 receptor on MSCs. This upregulation, which is involved in the migratory capacity of UCB-MSCs, was confirmed by siRNA inhibition and an antibody-neutralizing assay. In vivo survival experiments in orthotopic xenografted mice showed that MSC-based TRAIL gene delivery to irradiated tumors had greater therapeutic efficacy than a single treatment. These results suggest that clinically relevant tumor irradiation increases the therapeutic efficacy of MSC-TRAIL by increasing tropism of MSCs and TRAIL-induced apoptosis, which may be a more useful strategy for cancer gene therapy.
Variability Analysis of Inverter Delay Time
Authors: Aoki Masakazu , Hasegawa Ken , Itayama Keisuke , Yamawaki Taizo , Tanaka Satoshi 
Published: October 2010

AbstractThe variation characteristics of the inverter delay time in a large‐scale integrated (LSI) chip were evaluated by using an analytical model equation combined with the statistical simulation program with integrated circuit emphasis (SPICE) simulation as well as the experimental data obtained from a device matrix array (DMA) test vehicle 11. We demonstrate that the variation in the two‐stage delay time td (td = tdLH + tdHL) is linearly proportional to the variation of the effective peak operating current of the two‐stage inverter ID (7) expressed as Δtd/td = −ΔID/ID. This remains constant whether or not the current variation was induced by the threshold voltage variation (ΔVth), the current factor variation (Δβ), or both, even for unbalanced transistor width ratios between the p‐type metal oxide semiconductor (PMOS) and the n‐type metal oxide semiconductor (NMOS) (WP/WN = 0.5–5). Thus ΔID/ID can be regarded as a measure of the inverter delay time variation. The relative variation in the delay time increases dramatically when the supply voltage is decreased, which we attribute to the specific gate voltage dependence of the MOS drain current variation induced by the threshold voltage variation ΔVth. Copyright © 2010 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.
SAR ADC Architecture with Digital Error Correction
Authors: Hotta Masao , Kawakami Masayuki , Kobayashi Haruo , San Hao , Takai Nobukazu , Matsuura Tatsuji , Abe Akira , Yagi Katsuyoshi , Mori Toshihiko 
Published: October 2010

AbstractThis paper describes a high‐performance successive approximation register (SAR) analog to digital converter (ADC) using three comparators operating in parallel, instead of just one as in conventional ADCs. This comparator redundancy enables potentially faster operation, higher reliability, and comparator error correction. Reliability‐enhancement and error‐correction algorithms are described. We designed and implemented a 10‐bit, 6‐Msps SAR ADC with three comparators by using TSMC 0.18 µm CMOS technology. The advantages of high conversion rate and higher accuracy are verified by transistor‐level circuit simulation and experimental results of the test chip. Copyright © 2010 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.
Frequency Control of Photovoltaic–Diesel Hybrid System Connecting to Isolated Power Utility by Using Load Estimator and Energy Storage System
Authors: Datta Manoj , Senjyu Tomonobu , Yona Atsushi , Funabashi Toshihisa 
Published: October 2010

AbstractIn this paper, a control method based on a load power estimator and an energy storage system is proposed for isolated power utility‐connected photovoltaic–diesel hybrid system to provide frequency control. Here, photovoltaic power is controlled according to the load variation to minimize the frequency deviations. Load power is estimated by a minimal‐order observer. Then, a load variation index is calculated. A base photovoltaic power is produced from the available maximum photovoltaic power using a low‐pass filter and is added with the load variation index to generate the command photovoltaic power. Simulation results show that the proposed method is capable of providing frequency control and also delivers the photovoltaic power near the maximum level using an energy storage system. Copyright © 2010 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.
Dynamic Modeling and Neural Network Self‐tuning PID Control Design for a Linear Motor Driving Platform
Authors: Hsieh Sheng‐Ping , Hwang Thong‐Shing 
Published: October 2010

AbstractThe purpose of this paper is to design a wavelet neural network self‐tuning proportional–integral–derivative (PID) controller for solving the problem of fast changing payload in a linear motor in the 6‐DOF platform system. The wavelet neural network PID controller demonstrates the special capabilities of fast response and self‐tuning. A practical system identification method is proposed to identify the dynamic model of a linear motor by using the balancing realization theorem and inverse discrete Fourier transform. The experimental results of the real‐time software simulation and hardware‐in‐the‐loop simulation demonstrate the novel ability of self‐tuning PID controller for adapting the quick load change in the forcer of linear motor, which activates the platform system. Copyright © 2010 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.
An efficient MAC protocol with cooperative retransmission in mobile ad hoc networks
Authors: Chiang Jeng‐Long , Sheu Jang‐Ping , Tseng Huan‐Chun , Chen Wen‐Tsuen 
Published: October 2010

AbstractIn emerging wireless networks, cooperative retransmission is employed to replace packet retransmission between a pair of sender and receiver with poor channel condition. A cooperative MAC protocol which utilizes such benefit is proposed in this paper to improve the network performance in mobile ad hoc networks. In the proposed protocol, relay nodes between sender and receiver are used if the sender cannot communicate with the receiver reliably. Furthermore, the receiver may also stop forwarding the received data frame if the frame is received by the next‐hop receiver on the route to the final destination node. Simulation results show that the proposed protocol outperforms previous works in terms of increased transmission reliability and reduced delay time. Copyright © 2010 John Wiley & Sons, Ltd.
Radio resource management of self‐organizing OFDMA wireless mesh networks
Authors: Chu Feng Seng , Chen Kwang Cheng 
Published: October 2010

AbstractMesh networks with universal frequency reuse is expected to play an important role for future wireless system architecture, to provide ultra high throughput and seamless access via cooperative base stations (BSs), relays, and users. Different from traditional networks with central control, BSs should operate in a self‐organizing way (i) to support stable high‐throughput green communications (ii) to avoid inter/intra cell interference, and (iii) to implement in an ease and backward compatible way; and resource management is thus a critical step to satisfy (i), (ii), and (iii). We first systematically present radio resource management based on extensive literature survey. To optimize system performance, we develop the cognitive resource management for wireless mesh networks with self‐organizing BSs to utilize entire available spectrum, while BSs collect channel state information of each user by spectrum sensing. From numerical results, our resource management can significantly improve network throughput over the benchmark system, which suggests good potential for many wireless systems such as LTE‐A and WiMAX 2 that are adopting mesh network architecture, to achieve universal frequency reuse. Copyright © 2010 John Wiley & Sons, Ltd.