bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of two different alginate-based bioinks and the influence of melanoma growth within
Authors: Schipka Raphael , Heltmann-Meyer Stefanie , Schneidereit Dominik , Friedrich Oliver , Röder Jonas , Boccaccini Aldo R. , Schrüfer Stefan , Schubert Dirk W. , Horch Raymund E. , Bosserhoff Anja K. , Arkudas Andreas , Kengelbach-Weigand Annika , Schmid Rafael 
Published: June 2024

AbstractExtrusion-based bioprinting is an established method in biofabrication. Suitable bioinks have fundamentally different compositions and characteristics, which should be examined, in order to find a perfect model system. Here, we investigate the effect of two alginate-based, yet unalike 3D-printed bioinks, pre-crosslinked alginate-dialdehyde gelatin (ADA-GEL) and a mixture of alginate, hyaluronic acid, and gelatin (Alg/HA/Gel), on the melanoma cell line Mel Im and vice versa in terms of stiffness, shrinkage, cellular behavior and colony formation over 15 days. Rheological stiffness measurements revealed two soft gels with similar storage moduli. The cells did not have a significant impact on the overall stiffness, whereas ADA-GEL (2.5/2.5%) was significantly stiffer than Alg/HA/Gel (0.5/0.1/3%). Regarding the shrinkage of printed constructs, cells had a significant influence, especially in ADA-GEL, which has covalent bonds between the oxidized alginate and gelatin. Multi-photon microscopy exhibited proliferation, cell spreading and migration in ADA-GEL with cell–cell and cell–matrix interaction, dissimilarly to Alg/HA/Gel, in which cells formed spherical, encapsulated colonies. Scanning electron microscopy and histology showed degradation and multi-layered growth on ADA-GEL and fewer examples of escaped cells on Alg/HA/Gel. Both gels serve as proliferation bioink for melanoma with more necrosis in deeper Alg/HA/Gel colonies and differences in spreading and matrix interaction. These findings show the importance of proper characterization of the bioinks for different applications.
The SCREENIVF Hungarian version is a valid and reliable measure accurately predicting possible depression in female infertility patients
Authors: Szigeti F. Judit , Sexty Réka E. , Szabó Georgina , Kazinczi Csaba , Kéki Zsuzsanna , Sipos Miklós , Ujma Péter Przemyslaw , Purebl György 
Published: June 2024

AbstractInfertility patients, often in high distress, are entitled to being informed about their mental status compared to normative data. The objective of this study was to revalidate and test the accuracy of the SCREENIVF, a self-reported tool for screening psychological maladjustment in the assisted reproduction context. A cross-sectional, questionnaire-based online survey was carried out between December 2019 and February 2023 in a consecutive sample of female patients (N = 645, response rate 22.9%) in a university-based assisted reproduction center in Hungary. Confirmatory factor analysis and cluster and ROC analyses were applied to test validity, sensitivity and specificity in relation to Beck Depression Inventory (BDI) scores. Model fit was optimal (chi-square = 630.866, p < 0.001; comparative fit index = 0.99; root-mean-square error of approximation = 0.018 (90% CI 0.013–0.023); standardized-root-mean-square-residual = 0.044), and all dimensions were reliable (α > 0.80). A specific combination of cutoffs correctly predicted 87.4% of BDI-scores possibly indicative of moderate-to-severe depression (χ2(1) = 220.608, p < 0.001, Nagelkerke R2 = 0.462, J = 66.4). The Hungarian version of the SCREENIVF is a valid and reliable tool, with high accuracy in predicting BDI-scores. Low response rate may affect generalizability. The same instrument with different cutoffs can serve various clinical goals.
Machine learning survival prediction using tumor lipid metabolism genes for osteosarcoma
Authors: Li Shuai , Zheng Zhenzhong , Wang Bing 
Published: June 2024

AbstractOsteosarcoma is a primary malignant tumor that commonly affects children and adolescents, with a poor prognosis. The existence of tumor heterogeneity leads to different molecular subtypes and survival outcomes. Recently, lipid metabolism has been identified as a critical characteristic of cancer. Therefore, our study aims to identify osteosarcoma's lipid metabolism molecular subtype and develop a signature for survival outcome prediction. Four multicenter cohorts—TARGET-OS, GSE21257, GSE39058, and GSE16091—were amalgamated into a unified Meta-Cohort. Through consensus clustering, novel molecular subtypes within Meta-Cohort patients were delineated. Subsequent feature selection processes, encompassing analyses of differentially expressed genes between subtypes, univariate Cox analysis, and StepAIC, were employed to pinpoint biomarkers related to lipid metabolism in TARGET-OS. We selected the most effective algorithm for constructing a Lipid Metabolism-Related Signature (LMRS) by utilizing four machine-learning algorithms reconfigured into ten unique combinations. This selection was based on achieving the highest concordance index (C-index) in the test cohort of GSE21257, GSE39058, and GSE16091. We identified two distinct lipid metabolism molecular subtypes in osteosarcoma patients, C1 and C2, with significantly different survival rates. C1 is characterized by increased cholesterol, fatty acid synthesis, and ketone metabolism. In contrast, C2 focuses on steroid hormone biosynthesis, arachidonic acid, and glycerolipid and linoleic acid metabolism. Feature selection in the TARGET-OS identified 12 lipid metabolism genes, leading to a model predicting osteosarcoma patient survival. The LMRS, based on the 12 identified genes, consistently accurately predicted prognosis across TARGET-OS, testing cohorts, and Meta-Cohort. Incorporating 12 published signatures, LMRS showed robust and significantly superior predictive capability. Our results offer a promising tool to enhance the clinical management of osteosarcoma, potentially leading to improved clinical outcomes.
Identifying the origin of springs in weathered-fractured crystalline aquifers using a hydrogeophysical approach
Authors: Kouassi Kouassi Jean-Michel , Lachassagne Patrick , Mangoua Oi Mangoua Jules , Sombo Abé Parfait , Dibi Brou 
Published: June 2024

AbstractOver the last few decades, important advances have been made in the development of relevant hydrogeological conceptual models for crystalline aquifers, and notably for weathered-fractured crystalline aquifers. Paradoxically and contrary to other types of aquifers, these researches never aimed at characterizing springs, the places were groundwater naturally outflows from such aquifers. With such an objective, our methodological approach consisted first of a lithological and hydrogeological description of the aquifer system based on borehole data and outcrops in a representative weathered-fractured crystalline aquifer (Daloa, Ivory Coast). Next, electrical resistivity tomography (ERT) has been used (after validating the appropriate inversion method) to provide the imagery of the weathering profile both below the plateaus and in the valleys where the springs outflow. Piezometric and river discharge data were also processed notably to determine the direction of groundwater flow. Results demonstrate unambiguously that the isalterites aquifer supplies the springs, and that the underlying fractured layer is not directly implied in this supply. ERT combined with borehole and field lithological data also shows that the lateritic formations (alloterites) present near surface below the plateaus, as well as the upper part of the isalterites, were eroded in the valleys, but not deep enough to let the fractured layer outcrop. This conceptual model for springs not only provides a basis for characterizing such complex aquifers, but also provides technical guidance for spring catchment and groundwater protection in these crystalline areas.
A Bacillus velezensis strain isolated from oats with disease-preventing and growth-promoting properties
Authors: Cheng Chao , Su Shaofeng , Bo Suling , Zheng Chengzhong , Liu Chunfang , Zhang Linchong , Xu Songhe , Wang Xiaoyun , Gao Pengfei , Fan Kongxi , He Yiwei , Zhou Di , Gong Yanchun , Zhong Gang , Liu Zhiguo 
Published: June 2024

AbstractEndophytes have been shown to promote plant growth and health. In the present study, a Bacillus velezensis CH1 (CH1) strain was isolated and identified from high-quality oats, which was capable of producing indole-3-acetic acid (IAA) and strong biofilms, and capabilities in the nitrogen-fixing and iron carriers. CH1 has a 3920 kb chromosome with 47.3% GC content and 3776 code genes. Compared genome analysis showed that the largest proportion of the COG database was metabolism-related (44.79%), and 1135 out of 1508 genes were associated with the function “biosynthesis, transport, and catabolism of secondary metabolites.” Furthermore, thirteen gene clusters had been identified in CH1, which were responsible for the synthesis of fifteen secondary metabolites that exhibit antifungal and antibacterial properties. Additionally, the strain harbors genes involved in plant growth promotion, such as seven putative genes for IAA production, spermidine and polyamine synthase genes, along with multiple membrane-associated genes. The enrichment of these functions was strong evidence of the antimicrobial properties of strain CH1, which has the potential to be a biofertilizer for promoting oat growth and disease resistance.
Equivalent analytical model for liquid sloshing in a 2-D rectangular container with multiple vertical baffles by subdomain partition approach
Authors: Sun Ying , Meng Xun , Zhang Zhong , Gu Zhenyuan , Wang Jiadong , Zhou Ding 
Published: June 2024

AbstractAn equivalent analytical model of sloshing in a two-dimensional (2-D) rigid rectangular container equipped with multiple vertical baffles is presented. Firstly, according to the subdomain partition approach, the total liquid domain is partitioned into subdomains with the pure interface and boundary conditions. The separation of variables is utilized to achieve the velocity potential for subdomains. Then, sloshing characteristics are solved according to continuity and free surface conditions. According to the mode orthogonality of sloshing, the governing motion equation for sloshing under horizontal excitation is given by introducing generalized time coordinates. Besides, by producing the same hydrodynamic shear and overturning moment as those from the original container-liquid-baffle system, a mass-spring analytical model of the continuous liquid sloshing is established. The equivalent masses and corresponding locations are presented in the model. The feasibility of the present approach is verified by conducting comparative investigations. Finally, by utilizing normalized equivalent model parameters, the sloshing behaviors of the baffled container are investigated regarding baffle positions and heights as well as the liquid height, respectively.
Growth/differentiation factor 15 (GDF15) expression in the heart after myocardial infarction and cardioprotective effect of pre-ischemic rGDF15 administration
Authors: Dogon Geoffrey , Rigal Eve , Potel Eliot , Josse Marie , Rochette Luc , Bejot Yannick , Vergely Catherine 
Published: June 2024

AbstractGrowth/differentiation factor-15 (GDF15) is considered an unfavourable prognostic biomarker for cardiovascular disease in clinical data, while experimental studies suggest it has cardioprotective potential. This study focuses on the direct cardiac effects of GDF15 during ischemia–reperfusion injury in Wistar male rats, employing concentrations relevant to patients at high cardiovascular risk. Initially, we examined circulating levels and heart tissue expression of GDF15 in rats subjected to ischemia–reperfusion and sham operations in vivo. We then evaluated the cardiac effects of GDF15 both in vivo and ex vivo, administering recombinant GDF15 either before 30 min of ischemia (preconditioning) or at the onset of reperfusion (postconditioning). We compared infarct size and cardiac contractile recovery between control and rGDF15-treated rats. Contrary to our expectations, ischemia–reperfusion did not increase GDF15 plasma levels compared to sham-operated rats. However, cardiac protein and mRNA expression increased in the infarcted zone of the ischemic heart after 24 h of reperfusion. Notably, preconditioning with rGDF15 had a cardioprotective effect, reducing infarct size both in vivo (65 ± 5% in control vs. 42 ± 6% in rGDF15 groups) and ex vivo (60 ± 4% in control vs. 45 ± 4% in rGDF15 groups), while enhancing cardiac contractile recovery ex vivo. However, postconditioning with rGDF15 did not alter infarct size or the recovery of contractile parameters in vivo or ex vivo. These novel findings reveal that the short-term exogenous administration of rGDF15 before ischemia, at physiologically relevant levels, protects the heart against ischemia–reperfusion injury in both in vivo and ex vivo settings. The ex vivo results indicate that rGDF15 operates independently of the inflammatory, endocrine and nervous systems, suggesting direct and potent cardioprotective properties against ischemia–reperfusion injury.
Maturation of human cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) on polycaprolactone and polyurethane nanofibrous mats
Authors: Iwoń Zuzanna , Krogulec Ewelina , Tarnowska Inez , Łopianiak Iwona , Wojasiński Michał , Dobrzyń Agnieszka , Jastrzębska Elżbieta 
Published: June 2024

AbstractInvestigating the potential of human cardiomyocytes derived from induced pluripotent stem cells (iPSC-CMs) in in vitro heart models is essential to develop cardiac regenerative medicine. iPSC-CMs are immature with a fetal-like phenotype relative to cardiomyocytes in vivo. Literature indicates methods for enhancing the structural maturity of iPSC-CMs. Among these strategies, nanofibrous scaffolds offer more accurate mimicry of the functioning of cardiac tissue structures in the human body. However, further research is needed on the use of nanofibrous mats to understand their effects on iPSC-CMs. Our research aimed to evaluate the suitability of poly(ε-caprolactone) (PCL) and polyurethane (PU) nanofibrous mats with different elasticities as materials for the maturation of iPSC-CMs. Analysis of cell morphology and orientation and the expression levels of selected genes and proteins were performed to determine the effect of the type of nanofibrous mats on the maturation of iPSC-CMs after long-term (10-day) culture. Understanding the impact of 3D structural properties in in vitro cardiac models on induced pluripotent stem cell-derived cardiomyocyte maturation is crucial for advancing cardiac tissue engineering and regenerative medicine because it can help optimize conditions for obtaining more mature and functional human cardiomyocytes.
Decomposition of physical processes controlling EASM precipitation changes during the mid-Piacenzian: new insights into data–model integration
Authors: Sun Yong , Wu Haibin , Ding Lin , Chen Lixin , Stepanek Christian , Zhao Yan , Tan Ning , Su Baohuang , Yuan Xiayu , Zhang Wenchao , Liu Bo , Hunter Stephen , Haywood Alan , Abe-Ouchi Ayako , Otto-Bliesner Bette , Contoux Camille , Lunt Daniel J. , Dolan Aisling , Chandan Deepak , Lohmann Gerrit , Dowsett Harry , Tindall Julia , Baatsen Michiel , Peltier W. Richard , Li Qiang , Feng Ran , Salzmann Ulrich , Chan Wing-Le , Zhang Zhongshi , Williams Charles J. R. , Ramstein Gilles 
Published: June 2024

AbstractThe mid-Piacenzian warm period (MPWP, ~3.264–3.025 Ma) has gained widespread interest due to its partial analogy with future climate. However, quantitative data–model comparison of East Asian Summer Monsoon (EASM) precipitation during the MPWP is relatively rare, especially due to problems in decoding the imprint of physical processes to climate signals in the records. In this study, pollen-based precipitation records are reconstructed and compared to the multi-model ensemble mean of the Pliocene Model Intercomparison Project Phase 2 (PlioMIP2). We find spatially consistent precipitation increase in most simulations but a spatially divergent change in MPWP records. We reconcile proxy data and simulation by decomposing physical processes that control precipitation. Our results 1) reveal thermodynamic control of an overall enhancement of EASM precipitation and 2) highlight a distinct control of thermodynamic and dynamical processes on increases of tropical and subtropical EASM precipitation, reflecting the two pathways of water vapor supply that enhance EASM precipitation, respectively.
Should We Stop Using Lexical Diversity Measures in Children's Language Sample Analysis?
Authors: Ratner Nan Bernstein , Han Youngjin , Yang Ji Seung 
Published: June 2024

Purpose:
Prior work has identified weaknesses in commonly used indices of lexical diversity in spoken language samples, such as type–token ratio (TTR) due to sample size and elicitation variation, we explored whether TTR and other diversity measures, such as number of different words/100 (NDW), vocabulary diversity (VocD), and the moving average TTR would be more sensitive to child age and clinical status (typically developing [TD] or developmental language disorder [DLD]) if samples were obtained from standardized prompts.

Method:
We utilized archival data from the norming samples of the Test of Narrative Language and the Edmonton Narrative Norms Instrument. We examined lexical diversity and other linguistic properties of the samples, from a total of 1,048 children, ages 4–11 years; 798 of these were considered TD, whereas 250 were categorized as having a language learning disorder.

Results:
TTR was the least sensitive to child age or diagnostic group, with good potential to misidentify children with DLD as TD and TD children as having DLD. Growth slopes of NDW were shallow and not very sensitive to diagnostic grouping. The strongest performing measure was VocD. Mean length of utterance, TNW, and verbs/utterance did show both good growth trajectories and ability to distinguish between clinical and typical samples.

Conclusions:
This study, the largest and best controlled to date, re-affirms that TTR should not be used in clinical decision making with children. A second popular measure, NDW, is not measurably stronger in terms of its psychometric properties. Because the most sensitive measure of lexical diversity, VocD, is unlikely to gain popularity because of reliance on computer-assisted analysis, we suggest alternatives for the appraisal of children's expressive vocabulary skill.
An apparent inductance online identification for sensorless control of permanent magnet synchronous motor
Authors: Yang Ge , Weizhang Song , Wheeler Patrick , Yang Yang 
Published: June 2024

AbstractThe model‐based sensorless control of permanent magnet synchronous motor (PMSM) relies on the motor parameters. In practice, the considerable uncertainty of inductance and the difference between the apparent inductance and the incremental inductance exists. To overcome it, an online apparent inductance identification method for PMSM sensorless control is proposed. First, an initial incremental inductance table (IIT) is obtained offline. Then, an asymmetric high‐frequency square wave injection method is proposed to identify the incremental inductance at the operation point, and an adaptive minimum adjustment algorithm is proposed to adjust IIT automatically. Finally, the apparent inductance is calculated using the adjusted IIT. Based on it, the complete PMSM sensorless control is designed. The experimental results indicate this method is feasible and effective.
Wireless sensor network coverage optimization based on novel multi‐strategy gold rush optimizer
Authors: Kong Weibin , Cai Xin , Du Yi , Jia Baomin , Zhang Xiaoyu , Wang Lei , Cheng Ziyao , Shan Linyuan 
Published: June 2024

AbstractIn Wireless Sensor Networks (WSN), there are three conflicting important issues, including extensive coverage, low node redundancy, and energy consumption for secondary deployment. To balance network coverage and longevity, a WSN coverage model with three objectives is developed. A novel multi‐strategy gold rush optimizer is proposed for the coverage optimization problem in WSN. First, the fusion search method becomes extensive and flexible by combining the migration strategy with the Levy flight mechanism and joining a follower group. Then, the dynamic opposite learning strategy is used to increase population diversity in the panning stage. In addition, a multi‐player collaborative strategy is utilized to improve search ability of the algorithm in the collaboration stage. Finally, the random differential mutation method is used to try to assist the algorithm in escaping local optima. The simulation results show that this algorithm compared with other algorithms performs better on all three objectives considered.
Feedback-free starbursts at cosmic dawn
Authors: Li Zhaozhou , Dekel Avishai , Sarkar Kartick C. , Aung Han , Giavalisco Mauro , Mandelker Nir , Tacchella Sandro 
Published: June 2024

Aims. We extend the analysis of a physical model within the standard cosmology that robustly predicts a high star-formation efficiency (SFE) in massive galaxies at cosmic dawn due to feedback-free starbursts (FFBs). This model implies an excess of bright galaxies at z​ ≳ ​10 compared to the standard models based on the low SFE at later epochs, an excess that is indicated by JWST observations.
Methods. Here we provide observable predictions of galaxy properties based on the analytic FFB scenario. These can be compared with simulations and JWST observations. We use the model to approximate the SFE as a function of redshift and mass, assuming a maximum SFE of ϵmax​ = ​0.2 − 1 in the FFB regime. From this, we derive the evolution of the galaxy mass and luminosity functions as well as the cosmological evolution of stellar and star-formation densities. We then predict the star-formation history (SFH), galaxy sizes, outflows, gas fractions, metallicities, and dust attenuation, all as functions of mass and redshift in the FFB regime.
Results. The major distinguishing feature of the model is the occurrence of FFBs above a mass threshold that declines with redshift. The luminosities and star formation rates in bright galaxies are predicted to be in excess of extrapolations of standard empirical models and standard cosmological simulations, an excess that grows from z​ ∼ ​9 to higher redshifts. The FFB phase of ∼100 Myr is predicted to show a characteristic SFH that fluctuates on a timescale of ∼10 Myr. The stellar systems are compact (Re​ ∼ ​0.3 kpc at z​ ∼ ​10 and declining with z). The galactic gas consists of a steady wind driven by supernovae from earlier generations, with high outflow velocities (FWHM​ ∼ ​1400 − 6700 km s−1), low gas fractions (< 0.1), low metallicities (≲0.1 Z⊙), and low dust attenuation (AUV​ ∼ ​0.5 at z​ ∼ ​10 and declining with z). We make tentative comparisons with current JWST observations for initial insights, anticipating more complete and reliable datasets for detailed quantitative comparisons in the future. The FFB predictions are also offered in digital form.