bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phytotoxic effects of Echinochloa colona (L.) Link. (Poaceae) extracts on the germination and seedling growth of weeds
Authors: Gomaa N. H. , AbdElgawad H. R. 
Published: May 2012

This paper aims to study the phytotoxic potential of Echinochloacolonain order to explore its potential as bioherbicide. In this way, the phytotoxic effects of the most active fraction and pure compound from methanol (MeOH) extract of E.colona shoots on germination and seedling growth of ten weed species and on E. colona itself were evaluated. A lettuce seed bioassay indicated that dichloromethane (CH2Cl2) solublefraction of MeOH extract had the most phytotoxic activity. The most phytotoxic active compound of CH2Cl2 fraction was isolated and identified as tricin. Different concentrations of CH2Cl2 fraction (100, 500, 1000 mg L-1) and tricin (50, 100, 200 µmol L-1) were used to assess their phytotoxicity on germination and seedling growth of Portulaca oleracea, Corchorus olitorius, Brachiaria reptans, Euphorbia heterophylla, Dinebra retroflexa, Hibiscus trionum, Amaranthus graecizans, Amaranthus hybridus, Convolvulus arvensis, Setaria pumila and E.colona. Low concentration of CH2Cl2 fraction (100 mg L-1) significantly stimulated germination and seedling growth of some test species. There was no significant effect of the low concentration of tricin (50µmol L-1) on germination and elongation of roots and shoots of species. Higher concentrations of CH2Cl2 fraction (500, 1000 mg L-1) and tricin (100, 200µmol L-1) inhibited germination and seedling growth of most species. The results suggest that CH2Cl2 fraction and tricin might be potentially useful as bioherbicide for weed control in agriculture.
Endovascular Infections Caused by Histoplasma capsulatum: A Case Series and Review of the Literature
Authors: Ledtke Christopher , Rehm Susan J. , Fraser Thomas G. , Shrestha Nabin K. , Tan Carmela D. , Rodriguez E Rene , Tomford J Walton , Jain Anil , Lytle Bruce , Johnston Douglas , Sabik Joseph , Gordon Steven M. , van Duin David 
Published: May 2012

Context.—Endovascular infection is an uncommon but devastating manifestation of histoplasmosis, which is often diagnosed late in disease.
Objectives.—To evaluate the clinical and pathologic characteristics of patients with endovascular infections caused by Histoplasma capsulatum.
Design.—All cases of patients with documented endovascular histoplasmosis at a single tertiary care center in an endemic region during the period 1993–2010 were reviewed.
Results.—Patients presented with a subacute febrile illness and a history of endovascular devices. All patients had positive Histoplasma serology. Routine bacterial culture results were negative for all patients. In addition to yeast forms typical of histoplasmosis, pathologic findings also revealed mycelial forms in 4 of 5 patients. Inflammation was scant. Urinary antigen detection was positive in 4 of 5 patients and Histoplasma blood culture results were positive for 3 of 5 patients. Four patients were treated with a combination of surgical and medical therapy, which consisted of amphotericin B followed by itraconazole; these 4 patients had complete resolution of symptoms and no documented relapse. One patient died before planned surgery.
Conclusions.—Histoplasma capsulatum endovascular infections are clinically characterized by a subacute febrile illness with negative bacterial cultures in patients with prosthetic endografts or valves. Noninvasive diagnostics are often the initial clue to the diagnosis. Combined medical and surgical treatment is associated with survival. On histopathologic examination both mycelial and yeast forms are often observed, with absent to minimal tissue inflammatory reaction.
Evaluation of an Automated Digital Imaging System, Nextslide Digital Review Network, for Examination of Peripheral Blood Smears
Authors: Yu Hongbo , Ok Chi Young , Hesse Adam , Nordell Peter , Connor Diane , Sjostedt Erica , Pechet Liberto , Michael Snyder L 
Published: May 2012

Context.—Several automated digital imaging systems have been introduced in recent years to improve turnaround time and proficiency in examining peripheral blood smears in hematology laboratories.
Objective.—To evaluate a new automated digital imaging system, Nextslide Digital Review Network (Nextslide), for examination of peripheral blood smears.
Design.—We evaluated 479 peripheral blood smears, of which 247 (51.6%) were included for comparison of Nextslide and manual white blood cell differential counts and morphology evaluation, 204 (42.6%) were included for comparison of Nextslide and CellaVision (DM96) differential counts, and 28 (5.8%) were neonatal samples examined for enumeration of nucleated red blood cells.
Results.—Results from both method comparisons showed excellent correlation for all major white blood cell classes with correlation coefficients ranging from 0.70 to 0.99. Evaluation of white blood cell, red blood cell, and platelet morphology also showed good correlation among methods. White blood cell preclassification capability in the system was evaluated for rate and accuracy. Leukopenic samples demonstrated markedly decreased review time with Nextslide. Enumeration of nucleated red blood cells showed good correlation among methods.
Conclusions.—Our evaluation of Nextslide shows excellent correlation when compared with conventional manual differentials and CellaVision (DM96) differentials for evaluation of peripheral blood smears.
Reevaluation and Identification of the Best Immunohistochemical Panel (pVHL, Maspin, S100P, IMP-3) for Ductal Adenocarcinoma of the Pancreas
Authors: Liu Haiyan , Shi Jianhui , Anandan Vasuki , Wang Hanlin L. , Diehl David , Blansfield Joseph , Gerhard Glenn , Lin Fan 
Published: May 2012

Context.—Differentiation of ductal adenocarcinoma of the pancreas from nonneoplastic pancreatic tissues can be challenging, especially in small biopsy and fine-needle aspiration specimens.Objective.—To investigate the utility of 26 immunohistochemical markers (CAM 5.2, CK [cytokeratin] 7, CK20, CK17, CK19, MUC1, MUC2, MUC4, MUC5AC, MUC6, p53, DPC4/SMAD4, CDX2, pVHL [von Hippel-Lindau tumor suppressor gene protein], S100P, IMP-3 [insulin-like growth factor 2 messenger RNA binding protein 3], maspin, mesothelin, claudin 4, claudin 18, annexin A8, fascin, PSCA [prostate stem cell antigen], MOC31, CEA [carcinoembryonic antigen], and CA19-9 [cancer antigen 19-9]) in the diagnosis of ductal adenocarcinoma of the pancreas.Design.—Immunohistochemical staining for these markers was performed in 60 cases of pancreatic ductal adenocarcinoma on routine and tissue microarray sections. In addition, immunohistochemical staining for maspin, S100P, IMP-3, and pVHL was performed on cell blocks from 67 pancreatic fine-needle aspiration cases, including 44 cases of pancreatic ductal adenocarcinoma.Results.—The results demonstrated that (1) more than 90% of cases of ductal adenocarcinoma were positive for maspin, S100P, and IMP-3; (2) nearly all adenocarcinoma cases were negative for pVHL, whereas nonneoplastic ducts and acini were positive for pVHL in all cases; (3) normal/reactive pancreatic ducts were frequently positive for CK7, CK19, MUC1, MUC6, CA19-9, MOC31, PSCA, mesothelin, annexin A8, claudin 4, and claudin 18; (4) normal pancreatic ducts were usually negative for IMP-3, maspin, S100P, CK17, MUC2, MUC4, and MUC5AC; (5) 60% of adenocarcinomas were negative for DPC4/SMAD4; and (6) strong background staining was frequently seen with fascin, PSCA, and annexin A8.Conclusions.—pVHL, maspin, S100P, and IMP-3 constitute the best diagnostic panel of immunomarkers for confirming the diagnosis of pancreatic ductal adenocarcinoma in both surgical and fine-needle aspiration specimens.
CD68 Immunostaining in the Evaluation of Chronic Histiocytic Intervillositis
Authors: Heller Debra S. 
Published: May 2012

Context.—Chronic histiocytic intervillositis is an uncommon and poorly recognized lesion associated with poor perinatal outcomes, including intrauterine growth retardation and stillbirth. It has a high recurrence rate.
Objective.—To evaluate utility of CD68 immunostaining in the evaluation of chronic histiocytic intervillositis.
Design.—Institutional review board–approved retrospective review was performed. Cases were selected from the departmental archives of University Hospital, Newark, New Jersey, between 2002 and 2009. Controls were from second-trimester pregnancies with chromosomal abnormalities or multiple severe anomalies.
Results.—There were 9 cases and 11 controls. The mean CD68+ count per high-power field for the cases was 88 ± 23 (range, 51–180) and for the controls, 8 ± 5 (range, 0–24), P &lt; .001.
Conclusions.—This study establishes a range for histiocyte counts in chronic histiocytic intervillositis and pregnancies without chronic histiocytic intervillositis, and suggests that CD68 staining may have utility in the diagnosis, particularly for nonperinatal pathologists, who may be less familiar with this lesion.
Automated Quantification of MART1-Verified Ki67 Indices by Digital Image Analysis in Melanocytic Lesions
Authors: Nielsen Patricia Switten , Riber-Hansen Rikke , Raundahl Jakob , Steiniche Torben 
Published: May 2012

Context.—The proliferation marker Ki67 is an important diagnostic and prognostic aid in surgical pathology. However, manual quantification in a counting frame to accurately establish the proliferation rate (Ki67 index) is cumbersome and time-consuming. Instead, digital image analysis of Ki67/MART1 double stains may provide fast and novel index computations for entire tumor sections.Objectives.—To design and compare image analysis protocols that compute Ki67 indices of Ki67/MART1 double stains, to compare automated indices with previously published manual indices, and to compare the total number of proliferating cells (mimicking a Ki67 single stain) with the number of MART1-verified proliferating cells.Design.—Whole slide images were captured from 48 melanomas and 77 nevi stained with an immunohistochemical cocktail against Ki67 and MART1. Ki67 indices were determined by digital image analysis and different equations based on number or area.Results.—The differences between mean indices of melanomas and nevi were significant (P &lt; .001) in all index computations. Number-based image analysis of lesions with more than 250 melanocytic cells misclassified 1 of 42 melanomas and 4 of 53 nevi, numbers comparable with manual counting. Automated indices were significantly higher than manual indices, as were indices of mimicked Ki67 single stains compared with MART1-verified Ki67 indices (P &lt; .001).Conclusions.—Ki67 indices established by digital image analysis of Ki67/MART1 double stains demonstrated excellent abilities to discriminate melanomas from nevi with diagnostic performances equal to manually performed indices. Testing different definitions of the automated MART1-verified Ki67 index, no single definition stood out; thus, a variety of definitions may be used.
NLRP7 Mutation Analysis in Sporadic Hydatidiform Moles in Tunisian Patients: NLRP7 and Sporadic Mole
Authors: Landolsi Hanène , Rittore Cécile , Philibert Laurent , Hmissa Sihem , Gribaa Moez , Touitou Isabelle , Yacoubi Mohamed Tahar 
Published: May 2012

Context.—Hydatidiform mole, an aberrant human pregnancy, is commonly a nonrecurrent disease. Recently, a rare autosomal recessive form of familial and/or recurrent molar pregnancies was associated with mutations in the NLRP7 gene.
Objective.—To investigate whether NLRP7 mutations exist in Tunisian women with sporadic hydatidiform moles.
Design.—Genomic DNA from 38 unrelated Tunisian patients with sporadic hydatidiform moles were screened by sequencing all NLRP7 exons. A high-resolution melting curve analysis was performed on 170 DNA controls to analyze new sequence variants.
Results.—More than 13% of these patients were heterozygous for NLRP7 mutations. We found 2 novel missense mutations in the heterozygous state, c.544G&gt;A (p.Val182Met) in 1 patient and c.1480G&gt;A (p.Ala494Thr) in 2 patients, and 2 already reported mutations, c.1532A&gt;G (p.Lys511Arg) and c.2156C&gt;T (p.Ala719Val), in 2 patients. None of these mutations were identified in 170 controls except for 1 woman who was heterozygous for p.Val182Met.
Conclusion.—As homozygous NLRP7 mutations are associated with recurrent hydatidiform mole or conception loss, the heterozygous state could represent a risk factor for nonrecurrent mole.
Lymphovascular Invasion in Micropapillary Urothelial Carcinoma: A Study of 22 Cases
Authors: McQuitty Elizabeth , Ro Jae Y. , Truong Luan D. , Shen Steven S. , Zhai Qihui , Ayala Alberto G. 
Published: May 2012

Context.—Micropapillary urothelial carcinoma (MPUC) is a known aggressive variant of urothelial carcinoma. However, the reasons for its aggressiveness remain unclear.
Objective.—To investigate the frequency of lymphovascular invasion in 22 cases of MPUC.
Design.—Consecutive tissue sections were stained with D2-40 and CD34 to highlight lymphovascular channels associated with MPUC. Spaces containing tumor cells were scored as positive for lymphovascular invasion if the staining pattern on immunohistochemistry was distinct and circumferential.
Results.—Of 22 cases, 21 (95%) had lymphovascular invasion on immunohistochemical staining, with 91% lymphatic invasion and 4% vascular invasion. Interestingly, 8 cases were originally signed out as negative for lymphovascular invasion on the basis of hematoxylin-eosin–stained sections; of these, 7 (88%) had focal lymphovascular invasion evident on immunohistochemical staining.
Conclusions.—Our results confirm that micropapillary lacunae are not lymphovascular channels. However, nearly all MPUC tumors (95% in this series) have evidence of lymphovascular invasion by immunohistochemical analysis. Our data support the use of micropapillary features as a morphologic marker for lymphovascular invasion and MPUC as an adverse histologic type of urothelial carcinoma.
Efficacy and safety of oral and parenteral methotrexate therapy in children with juvenile idiopathic arthritis: An observational study with patients from the German Methotrexate Registry
Authors: Klein A. , Kaul I. , Foeldvari I. , Ganser G. , Urban A. , Horneff G. 
Published: May 2012

AbstractObjectiveThe German Methotrexate Registry has been collecting data concerning the efficacy and safety of methotrexate (MTX) treatment since 2005. The aim of this retrospective analysis is to compare oral and parenteral MTX treatment regarding efficacy and safety.MethodsInclusion criteria were diagnosis of juvenile idiopathic arthritis, MTX treatment for at least 6 months, a consistent route of administration of MTX, and no previous or concomitant treatment with biologic agents. Efficacy was measured by the American College of Rheumatology (ACR) pediatric (Pedi) criteria. Primary outcome was efficacy defined as the number of patients reaching ACR Pedi 30 improvement criteria after 6 months of treatment. Secondary outcome criteria were the ACR Pedi 50 and Pedi 70 criteria at 6 and 12 months, respectively. Analyses were performed with the intent‐to‐treat population.ResultsOf the 411 eligible patients, 259 (63%) received oral MTX and 152 (37%) received subcutaneous MTX. In both patient groups, a comparable weekly dose of MTX (0.4 mg/kg versus 0.42 mg/kg) was used, and a comparable number of patients received concomitant steroids. The primary outcome in both treatment groups was that a comparably high number of patients showed a clinical response according to the ACR Pedi 30 score after 6 months of treatment (73% versus 72%; P = 0.87). Twenty‐two percent of patients with oral therapy and 27% with subcutaneous therapy had at least 1 documented adverse event. Discontinuation of treatment was observed in both groups with equal frequency, while significantly more patients with subcutaneous application discontinued MTX because of adverse events (11% versus 5%; P = 0.02).ConclusionIn this retrospective analysis, parenteral MTX was not superior to oral administration regarding efficacy and tolerability.
Alkylboronic Esters from Palladium‐ and Nickel‐Catalyzed Borylation of Primary and Secondary Alkyl Bromides
Authors: Yi Jun , Liu Jin‐Hui , Liang Jun , Dai Jian‐Jun , Yang Chu‐Ting , Fu Yao , Liu Lei 
Published: May 2012

AbstractPalladium‐ and nickel‐catalyzed cross‐coupling recations of unactivated alkyl bromides with diboron reagents have been developed as practical methods for the synthesis of primary and secondary alkylboronic esters. These reactions extend the concept and utility of Pd‐ and Ni‐catalyzed cross‐coupling of aliphatic electrophiles. They also show different substrate selectivity and ligand dependence as compared to the recently reported Cu‐catalyzed borylation reaction.
Cyclohexa‐3,5‐diene‐1,2‐trans‐diols – Synthesis and Application in Target‐Oriented Syntheses
Authors: Hausmann Tobias , Pietruszka Jörg , Schumacher Sebastian 
Published: May 2012

AbstractAn exhaustive overview of the field of cyclohexa‐3,5‐diene‐1,2‐trans‐diols is given. Early and recent methods for the formation of the compounds are reviewed and the various syntheses in which the title compounds have been applied are presented. Special emphasis is given to naturally occurring epoxides, which have been the dominant target molecules since the 1970s. Finally, recent advances in biotechnology are highlighted; with the increased availability of the enantiomerically pure cyclohexa‐3,5‐diene‐1,2‐trans‐diols, new synthetic endeavours were initiated.
Conditioned Medium from Chondrocyte/Scaffold Constructs Induced Chondrogenic Differentiation of Bone Marrow Stromal Cells
Authors: Liu Jinchun , Liu Xia , Zhou Guangdong , Xiao Ran , Cao Yilin 
Published: May 2012

AbstractFor the application of bone marrow stromal cells (BMSCs) in cartilage tissue engineering, it is imperative to develop efficient strategies for their chondrogenic differentiation. In this study, the conditioned media derived from chondrocyte/scaffold constructs were used to direct chondrogenic differentiation of BMSCs. The porcine articular chondrocytes were seeded on the PGA/PLA scaffolds to form chondrocyte/scaffold constructs and were cultured to form engineered cartilage in vitro. The culture media were collected as conditioned media and used for chondrogenic induction of BMSC pellets (experimental group, Exp.). The chondrocyte pellets and BMSC pellets were cultured routinely as positive control (PC) and negative control (NC), respectively. After 4 weeks, the wet weight and GAG content in Exp. group and PC group were significantly higher than that in NC group. Histological and immunohistochemical analysis showed that cartilaginous tissue was formed with typical cartilage lacuna structure and positive staining of collagen Type II (Col II) in the peripheral area of the BMSC pellets in Exp. group. Gene expression of Sox9, Col II, and COMP in Exp. group and PC group were significantly higher than that in NC group. The growth factors in the conditioned media derived from human costal chondrocytes‐scaffold constructs were tested by protein microassay. The conditioned media contained low levels of TGF‐β1,2,3, IGF‐1 and high levels of IGF‐2, FGF‐4, and IGFBP4,6, and so forth. The soluble factors derived from the engineered cartilage can induce chondrogenic differentiation of BMSCs independently. Many cytokines may function in chondrogenesis in a coordinated way. Anat Rec, 2012. © 2012 Wiley Periodicals, Inc.
Combined modality treatment for patients with locally advanced pancreatic adenocarcinoma
Authors: Cantore M , Girelli R , Mambrini A , Frigerio I , Boz G , Salvia R , Giardino A , Orlandi M , Auriemma A , Bassi C 
Published: May 2012

AbstractBackgroundRadiofrequency ablation (RFA) is an emerging treatment for patients with locally advanced pancreatic carcinoma, and can be combined with radiochemotherapy and intra-arterial plus systemic chemotherapy.MethodsThis observational study compared two groups of patients with locally advanced pancreatic carcinoma treated with either primary RFA (group 1) or RFA following any other primary treatment (group 2).ResultsBetween February 2007 and May 2010, 107 consecutive patients were treated with RFA. There were 47 patients in group 1 and 60 in group 2. Median overall survival was 25·6 months. Median overall survival was significantly shorter in group 1 than in group 2 (14·7 versus 25·6 months; P = 0·004) Patients treated with RFA, radiochemotherapy and intra-arterial plus systemic chemotherapy (triple-approach strategy) had a median overall survival of 34·0 months.ConclusionRFA after alternative primary treatment was associated with prolonged survival. This was further extended by use of a triple-approach strategy in selected patients. Further evaluation of this approach seems warranted.
The use of black and white infrared photography for recording blunt force injury
Authors: Bernstein Mark , Nichols George , Blair Jeff 
Published: May 2012

AbstractInfrared (IR) wavelengths penetrate skin and can selectively image volumes of subsurface blood. Twenty‐eight blunt force injuries on nine decedents were photographed with color and IR film to compare the ability of each to image the injury. Of the 28 injuries, 10 were clinically interpreted as contusions, 4 as abrasions, 10 as abraded contusions, and 4 as erythematous discolorations, nos. Twenty‐four of these injuries were incised to determine the presence and extent of subsurface bleeding. It was found that contusion had been clinically missed in five cases, three because they were hidden by abrasion and two because they appeared only as a mild cutaneous erythema. All five cases were detected by IR and verified by incision. Melanin pigmentation contributed to masking of contusion in one case and postmortem lividity confounded interpretation of contusion in one case. In all 14 injuries featuring abrasion as a component, IR de‐emphasized or eliminated the visibility of abrasion. A single false negative was reported in which a visible contusion, verified by incision, was not seen with IR. In comparing injury patterns, color and IR renditions were dissimilar in a number of cases reflecting the difference between the manner in which light and IR photography imaged the depth and volume of blood within an injury. In summary, IR imaging of wounds can discover hidden bruises, verify clinical diagnosis of bruises, and augment interpretation of wound patterns. Clin. Anat. 2013. © 2012 Wiley Periodicals, Inc.
Detail‐aware spatial deformation transfer
Authors: Yoshiyasu Yusuke , Yamazaki Nobutoshi 
Published: May 2012

ABSTRACTIn this paper, we propose a deformation transfer method that is applicable to multi‐component objects and is able to transfer fine‐scale deformations. To accomplish our goal, we combine surface‐based and space‐based deformation transfers. Our system enhances the result of space deformation transfer, which is applicable to multi‐component objects but loses details, by using surface‐based deformation transfer to add fine‐scale deformations. Self‐collisions are handled by approximating spatial relationships between surfaces, from the result of space deformation. Experimental results show that our method can transfer fine‐scale deformations such as motions of a skirt and muscle bulging. Copyright © 2012 John Wiley & Sons, Ltd.