bluuebunny/crossref_metadata_2025_split huggingface dataset:
Age of blood as a limitation for transfusion: potential impact on blood inventory and availability
Authors: Fontaine Magali J. , Chung Yenho T. , Erhun Feryal , Goodnough Lawrence T. 
Published: May 2010

BACKGROUND: Evolving concerns about storage lesions for red blood cells (RBCs) have led to ongoing trials evaluating the benefits of transfusing fresher blood to acutely ill patients.STUDY DESIGN AND METHODS: We evaluated several RBC maximum shelf lives (MSLs) and their impact on RBC availability and outdate rate. First, we determined the mean age of the RBC units in our inventory by analyzing the data set of 18,987 nonirradiated RBC units transfused at our institution from April 2008 to March 2009. Second, we determined the feasibility of issuing RBC units of a designated age to patients using the same data set. We defined six scenarios where RBC units have different MSLs: Scenarios 1, 2, 3, 4, and 5 used a MSL of 7, 14, 21, 28, and 35 days, respectively. Scenario 6 used a combination of different MSLs depending on the category of patients.RESULTS: RBC units spent on average 8.6 days on the shelf with a mean age of 10.2 days at delivery and 18.8 days at issue for transfusion. Using the original 18,987 data points, we observed a shortfall in the availability of RBC units, decreased by 51, 20, 10, 4, 1, and 0% and an increase in the outdate rate to 3.2, 2.2, 1.0, 0.6, 0.4, and 4.5% for Scenarios 1, 2, 3, 4, 5, and 6, respectively, compared to baseline.CONCLUSION: Changing the MSL for RBC units would require novel approaches to RBC inventory management to meet hospital demands with acceptable outdate rates.
In vitro and in vivo evaluation of apheresis platelets stored for 5 days in 65% platelet additive solution/35% plasma
Authors: Vassallo Ralph R. , Adamson John W. , Gottschall Jerome L. , Snyder Edward L. , Lee Wanda , Houghton Jaime , Elfath M. Dean 
Published: May 2010

BACKGROUND: In the United States, apheresis platelets (PLTs) are suspended in autologous plasma. PLT additive solutions, long used in Europe, decrease recipient allergic reactions and may reduce the risk of transfusion‐related acute lung injury. We evaluated Amicus‐collected PLTs stored in platelet additive solution (PAS) III (InterSol) for 5 days.STUDY DESIGN AND METHODS: In Study 1, 71 subjects donated two products on a single day—one each stored in 100% plasma or 65% PAS III/35% plasma. Products underwent standard in vitro testing on Days 1 and 5. In Study 2, 43 additional subjects provided Amicus products stored for 5 days in 65% PAS III/35% plasma for in vivo radiolabeled recovery and survival determinations. The effect of approximately 2500 cGy Day 1 gamma irradiation was evaluated in a subset of products.RESULTS: PAS III PLTs (n = 70) had a median Day 5 pH22°C of 7.2 (lower 95%, 95% tolerance limit, 6.9). Mean Day 5 recovery and survival of radiolabeled PAS III PLTs (n = 33) were, respectively, 80.5 and 72.1%, of fresh autologous PLTs. With 95% confidence, these values were at least 66% of fresh PLT recovery and 58% of survival. All in vitro variables remained within ranges seen in licensed products for irradiated and nonirradiated PAS III PLTs.CONCLUSION: Leukoreduced Amicus PLTs stored in 65% PAS III/35% plasma in PL‐2410 containers maintained pH ≥ 6.9 throughout 5 days' storage. Radiolabeled PLT recovery and survival values met US Food and Drug Administration statistical criteria. Gamma‐irradiated PAS III PLTs demonstrated no significant adverse effects due to irradiation in in vitro testing.
An improved red blood cell additive solution maintains 2,3‐diphosphoglycerate and adenosine triphosphate levels by an enhancing effect on phosphofructokinase activity during cold storage
Authors: Burger Patrick , Korsten Herbert , De Korte Dirk , Rombout Eva , Van Bruggen Robin , Verhoeven Arthur J. 
Published: May 2010

BACKGROUND: Current additive solutions (ASs) for red blood cells (RBCs) do not maintain constant 2,3‐diphosphoglycerate (DPG) and adenosine triphosphate (ATP) levels during cold storage. We have previously shown that with a new AS called phosphate‐adenine‐glucose‐guanosine‐gluconate‐mannitol (PAGGGM), both 2,3‐DPG and ATP could be maintained throughout storage for 35 days.STUDY DESIGN AND METHODS: In this study, the mechanism underlying the effect of PAGGGM on RBC storage was studied in more detail. By using double‐erythrocytapheresis units (leukoreduced), a direct comparison could be made between the current AS saline‐adenine‐glucose‐mannitol (SAGM) and the experimental solution PAGGGM. During cold storage, several in vitro characteristics were analyzed.RESULTS: In agreement with our previous findings with single RBCs, PAGGGM maintained 2,3‐DPG and ATP levels for 35 days of cold storage. Furthermore, glucose consumption and lactate production were higher in PAGGGM units during the first 21 days of cold storage. Fructose‐1,6‐diphophate and dihydroxyacetone phosphate levels were also increased during the first 21 days of storage in PAGGGM units.CONCLUSION: These results indicate that it is likely that phosphofructokinase (PFK) activity is enhanced in PAGGGM units relative to SAGM units. After 21 days, PFK activity also decreases in PAGGGM units, but sufficient metabolic reserve in these units prevents depletion of 2,3‐DPG and ATP.
Epitope‐based matching for HLA‐alloimmunized platelet refractoriness in patients with hematologic diseases
Authors: Pai Shun‐Chung , Lo Shyh‐Chyi , Lin Tsai Su‐Jen , Chang Ji‐Sheng , Lin Dong‐Tsamn , Lin Kuo‐Sin , Lin Liang‐In 
Published: May 2010

BACKGROUND: For HLA‐alloimmunized patients, platelet (PLT) concentrations are provided either at matched HLA‐A and HLA‐B loci or by serologic cross‐reactivity groups (CREG) matching strategy. However, this method has some limitations.STUDY DESIGN AND METHODS: In this study, the epitope‐based matching (EBM) method was evaluated for selecting proper HLA‐typed PLTs for patients with PLT transfusion refractoriness. Bead‐based single‐antigen HLA antibody detection method and HLAMatchmaker software were used to define the epitopes recognized by HLA‐specific antibodies and to select compatible PLTs for nine patients with alloimmunized refractoriness. Corrected count increments (CCIs) were prospectively determined to compare successful transfusion rates among different matching methods in 142 PLT transfusions. In addition, HLA antibodies were serially detected to see whether any emerging antibodies appeared after receiving the EBM‐matched PLTs.RESULTS: The transfusion success rates evaluated with 1‐hour CCIs for perfect matching or lacking any mismatching at HLA‐A and ‐B locus (A/BU)‐matched, CREG‐matched, and EBM‐matched PLTs were 85.2, 63.2, and 83.7%, respectively. Compared to CREG‐matched PLTs, EBM‐matched PLTs showed better transfusion results (p = 0.035). In the follow‐up study (7 months; range, 3‐13 months), no emerging HLA‐specific antibodies were detected after receiving EBM‐matched PLTs.CONCLUSIONS: EBM performed on the basis of bead‐based single‐antigen HLA antibody detection coupled with the HLAMatchmaker program is recommended in choosing proper PLTs for refractory patients when A/BU‐matched PLTs were not available.
The cost‐effectiveness of pathogen reduction technology as assessed using a multiple risk reduction model
Authors: Custer Brian , Agapova Maria , Martinez Rebecca Havlir 
Published: May 2010

BACKGROUND: Pathogen reduction technology (PRT) for labile blood components has the potential to reduce the risk of many adverse events associated with transfusion. Because of the potential broad‐spectrum risk reduction capability of PRT, the health economics of PRT could be an important consideration in decision making for this technology.STUDY DESIGN AND METHODS: Decision analytic models comparing current blood safety screens and interventions to riboflavin‐based whole blood PRT (currently in development) and separately to platelets (PLTs)‐and‐plasma PRT from the health care system perspective in Canada were used to assess the cost‐utility of PRT in reducing the following adverse events: human immunodeficiency virus, hepatitis B virus, hepatitis C virus, human T‐lymphotropic virus, syphilis, West Nile virus, bacteria, Chikungunya virus, cytomegalovirus, Trypanosoma cruzi, graft‐versus‐host disease, febrile nonhemolytic transfusion reactions, and transfusion‐related immunomodulation. PRT was modeled as an addition to rather than a replacement for current interventions. The potential of PRT to reduce the risk of an unknown pathogen was not assessed.RESULTS: Whole blood PRT was estimated to have a cost‐effectiveness of $1,276,000/quality‐adjusted life‐year (QALY; 95% confidence interval [CI] approximation, 600,000‐3,313,000) compared to current screens and interventions. PLTs‐and‐plasma PRT was estimated to have a cost‐effectiveness of $1,423,000/QALY (95% CI approximation, 834,000‐2,818,000) on an all‐transfusions basis.CONCLUSIONS: Because of the complexity of transfusion risks and practices, the cost‐effectiveness of whole blood or PLTs‐and‐plasma PRT can be modeled provided that assumptions and simplifications are made. Uncertainty remains with respect to the risk reduction that can be achieved for some adverse events. Nevertheless, the results of this cost‐effectiveness analysis can be used to inform policy decisions regarding PRT technology in the context of other initiatives designed to improve transfusion safety.
Nonlethal, attenuated, transfusion‐associated graft‐versus‐host disease in an immunocompromised child: case report and review of the literature
Authors: Neves João F. , Marques António , Valente Rosalina , Barata Deolinda 
Published: May 2010

BACKGROUND: Transfusion‐associated graft‐versus‐host disease (TA‐GVHD) is a rare complication of transfusion of nonirradiated blood components. It usually affects children in high‐risk groups, including those who have primary immunodeficiencies (PIDs). It usually presents with skin, hepatic, digestive, and hematologic involvement and is normally fatal.CASE REPORT: We report the case of a nonlethal, attenuated, TA‐GVHD in a 7‐month‐old boy. The disease was marked by the presence of a severe rash but lacked all the other usual manifestations. We speculate that the unusually benign course of this disease, which has normally a fulminant course, was due to the fact that this child was under high‐dose corticotherapy at the time of the engraftment. This fortunate coincidence led to the survival of this child and allowed the diagnosis of a combined immunodeficiency.CONCLUSION: A high index of suspicion is required for the diagnosis and proper management of PID. The administration of nonirradiated blood components in the first year of life, sometimes before the clinical suspicion of a PID, is of great concern. TA‐GVHD may be more prevalent than reported in the literature and it is possibly a nonidentified cause of death in recipients with unexplained death and nondiagnosed PID.
¿Está adoptando Asia la «flexiseguridad»?
Authors: VANDENBERG Paul 
Published: May 2010

Resumen.Incitados por la competencia mundial, muchos países están adaptando la reglamentación laboral al principio de la «flexiseguridad», que da a las empresas más flexibilidad para contratar y despedir a los trabajadores, al tiempo que ampara a éstos cuando deben pasar de un empleo a otro. El presente estudio de seis países asiáticos indica que China y República de Corea han promulgado reformas de este género; Singapur y Malasia han implantado algunos elementos de «flexiseguridad», pero no un seguro de desempleo, y la India y Sri Lanka han hecho pocas reformas y mantienen un modelo anticuado en el que la protección depende de la empresa. Para prestar apoyo a los trabajadores del sector informal, China, India y Sri Lanka se valen de obras públicas y de programas de fomento del trabajo por cuenta propia y de formación profesional.
Los efectos de la inestabilidad laboral en el trabajador y la dependencia del empleo
Authors: SORA Beatriz , CABALLER Amparo , PEIRÓ José María 
Published: May 2010

Resumen.La globalización y el avance de la competencia internacional van acompañados de unos regímenes de contratación más flexibles y un aumento de la inestabilidad del empleo. Los autores estudian los efectos de la inseguridad laboral que sienten los trabajadores en sus propias actitudes e intenciones en el trabajo. Tras pasar revista a las investigaciones realizadas acerca de la teoría del estrés y las consecuencias de la inestabilidad laboral, ponen a prueba dos hipótesis en 942 trabajadores españoles. La primera es que la inestabilidad disminuye la satisfacción con el trabajo y la entrega a la empresa, a la vez que alienta la intención de abandonar ésta. La segunda es que la sensación de inseguridad, la necesidad económica y la «empleabilidad» están interrelacionadas e influyen en la actitud del trabajador.
Agricultural extension and imperfect supervision in contract farming: evidence from Madagascar
Authors: Bellemare Marc F. 
Published: May 2010

AbstractThis article tests whether agricultural extension and imperfect supervision—conflated here into the number of visits by a technical assistant—increase productivity in a sample of contract farming arrangements between a processing firm and small agricultural producers in Madagascar. Production functions are estimated which treat the number of visits by a technical assistant as an input and which exploit the variation in the number of visits between the contracted crops grown on a given plot by a specific grower, thereby accounting for district‐, grower‐, and plot‐level unobserved heterogeneity. Results indicate that the elasticity of yield with respect to the number of visits lies between 1.3 and 1.7.
Exchange rate uncertainty and agricultural trade: panel cointegration analysis for Turkey
Authors: Erdem Ekrem , Nazlioglu Saban , Erdem Cumhur 
Published: May 2010

AbstractThis study addresses the impact of exchange rate levels and uncertainty on bilateral agricultural trade between Turkey and 20 major trading partners, using panel cointegration analysis for annual data covering the period 1980–2005. Results show that (i) the exchange rate level is less robustly linked to trade volumes than is exchange rate uncertainty, (ii) exchange rate uncertainty is associated with smaller trade volumes in both directions, but more so for imports than for exports, and (iii) income growth in Turkey is associated with greater imports, just as income growth among trading partners is associated with greater exports. These findings provide some clues for understanding the recent patterns of Turkish agricultural trade, and suggest directions for future research.
Histological, histomorphometrical, and radiological evaluation of an experimental implant design with a high insertion torque
Authors: Duyck Joke , Corpas Livía , Vermeiren Stephanie , Ogawa Toru , Quirynen Marc , Vandamme Katleen , Jacobs Reinhilde , Naert Ignace 
Published: May 2010

AbstractObjectives: The aim of this study was to compare bone behaviour around an experimental implant design with a high insertion torque with the Astra‐Tech© implant (control).Materials and methods: In ten 18‐month‐old male minipigs, the last premolars and first molars were extracted to provide space for two implants in each quadrant. A first set of 40 implants were placed 3 months after the extraction and 40 additional implants were installed another 2 months later. The animals were sacrificed 3 months after the first implant installation so that half of the implants had healed for 1 month and the other half for 3 months. Radiological evaluation was performed at baseline, 1 month, 2 months, and 3 months after implant installation. Bone defect depth and area, bone level changes, bone‐to‐implant contact density, and peri‐implant bone fraction were measured histomorphometrically.Results: Radiological analyses revealed a significantly higher bone loss around the experimental implants. Histomorphometric analyses confirmed significantly more bone loss, larger marginal bone defects, and a lower overall peri‐implant bone fraction around the experimental implants.Conclusion: The experimental implant design caused significantly more peri‐implant bone loss compared with the control implant. As strain gauge measurements indicate excessive marginal strains around the experimental implants, osseocompression might have played a role in the observed marginal bone loss. To cite this article:
Duyck J, Corpas L, Vermeiren S, Ogawa T, Quirynen M, Vandamme K, Jacobs R, Naert I. Histological, histomorphometrical, and radiological evaluation of an experimental implant design with a high insertion torque. Clin. Oral Impl. Res. 21, 2010; 877–884.
Discomfort experienced after immediate implant placement associated with three different regenerative techniques
Authors: Urban Thomas , Wenzel Ann 
Published: May 2010

AbstractObjectives: To assess patient discomfort in terms of pain, swelling and bleeding following immediate implant placement in molar regions using one of three regenerative techniques.Methods: Ninety‐two patients (44 women and 48 men; mean age 50 years [range 23–77], 35 smokers and 57 non‐smokers) in need of a single implant crown to replace a molar were included. After placing the implant large periimplant defects were present. These defects were treated either with autologous bone (AB) chips, Ossix membrane (OM) or a combination of AB chips and OM. The postoperative course was recorded on 100 mm visual analogue scales (VAS) with extreme end points for the level of pain on the day of surgery, 1, 2 and 3 days postoperatively (no/extreme pain); swelling on the day of surgery, 1, 2 and 3 days postoperatively (no/severe swelling); and bleeding from the wound on the day of surgery, 1, 2 and 3 days postoperatively (no/severe bleeding).Results: Pain peaked 5–6 h postoperatively (mean VAS=25). Swelling (mean VAS=62) and oozing from the wound (mean VAS=13) peaked 1 day postoperatively. There were no significant differences in the perception of pain, swelling or oozing from the wound between the three regenerative groups. The VAS score for pain was higher for smokers than for non‐smokers at all times; a significant difference was found from 1 day until 3 days postoperatively (0.011<P<0.048). All pain scores, except the score for the day of the operation, were significantly higher in younger (<50 years) than in older patients (≥50 years) (0.002≤P≤0.49).Conclusions: Patients experienced little to moderate pain in combination with marginally severe swelling and mild oozing after immediate implant placement in molar regions involving regenerative techniques. Being a smoker was associated with more pain in contrast to being >50 years, which was associated with less pain. To cite this article:
Urban T, Wenzel A. Discomfort experienced after immediate implant placement associated with three different regenerative techniques. Clin. Oral Impl. Res. 21, 2010; 1271–1277. doi: 10.1111/j.1600‐0501.2010.01943.x
Osteoblast response to magnesium ion‐incorporated nanoporous titanium oxide surfaces
Authors: Park Jin‐Woo , Kim Youn‐Jeong , Jang Je‐Hee , Song Hwangjun 
Published: May 2010

AbstractObjective: This study investigated the surface characteristics and in vitro osteoconductivity of a titanium (Ti) surface incorporated with the magnesium ions (Mg) produced by hydrothermal treatment for future application as an endosseous implant surface.Material and methods: Mg‐incorporated Ti oxide surfaces were produced by hydrothermal treatment using Mg‐containing solution on two different microstructured surfaces – abraded minimally rough (Ma) or grit‐blasted moderately rough (RBM) samples. The surface characteristics were evaluated using scanning electron microscopy, thin‐film X‐ray diffractometry, X‐ray photoelectron spectroscopy, optical profilometry, and inductively coupled plasma atomic emission spectroscopy (ICP‐AES). MC3T3‐E1 pre‐osteoblast cell attachment, proliferation, alkaline phosphatase (ALP) activity, and quantitative analysis of osteoblastic gene expression on Ma, RBM, Mg‐incorporated Ma (Mg), and Mg‐incorporated grit‐blasted (RBM/Mg) Ti surfaces were evaluated.Results: Hydrothermal treatment produced an Mg‐incorporated Ti oxide layer with nanoporous surface structures. Mg‐incorporated surfaces showed surface morphologies and surface roughness values almost identical to those of untreated smooth or micro‐rough surfaces at the micron scale. ICP‐AES analysis showed Mg ions released from treated surfaces into the solution. Mg incorporation significantly increased cellular attachment (P=0 at 0.5 h, P=0.01 at 1 h) on smooth surfaces, but no differences were found on micro‐rough surfaces. Mg incorporation further increased ALP activity in cells grown on both smooth and micro‐rough surfaces at 7 and 14 days of culture (P=0). Real‐time polymerase chain reaction analysis showed higher mRNA expressions of the osteoblast transcription factor gene (Dlx5), various integrins, and the osteoblast phenotype genes (ALP, bone sialoprotein and osteocalcin) in cells grown on micro‐rough (RBM) and Mg‐incorporated (Mg and RBM/Mg) surfaces than those on Ma surfaces. Mg incorporation further increased the mRNA expressions of key osteoblast genes and integrins (α1, α2, α5, and β1) in cells grown on both the smooth and the micro‐rough surfaces.Conclusion: These results indicate that an Mg‐incorporated nanoporous Ti oxide surface produced by hydrothermal treatment may improve implant bone healing by enhancing the attachment and differentiation of osteoblastic cells. To cite this article: Park J‐W, Kim Y‐J, Jang J‐H, Song H. Osteoblast response to magnesium ion‐incorporated nanoporous titanium oxide surfaces. Clin. Oral Impl. Res. 21, 2010; 1278–1287. doi: 10.1111/j.1600‐0501.2010.01944.x