bluuebunny/crossref_metadata_2025_split huggingface dataset:
A two‐dimensional electrophoresis reference map for the bovine placenta during late pregnancy
Authors: Kim Hong Rye , Han Rong Xun , Yoon Jong Taek , Park Chang Sik , Jin Dong Il 
Published: November 2009

AbstractAn understanding of bovine placental gene expression is essential for the study of animal reproductive physiology. Recent reports have found that placental abnormalities occur frequently in cloned bovines and mice. However, the molecular mechanisms underlying bovine placenta function remain unclear. Here, we present a preliminary description of the bovine placenta proteome. Proteins within the isoelectric point ranging from 4.0 to 7.0 and 6.0 to 9.0 were analyzed separately using 2‐DE, using three replicates of bovine placenta. Approximately 2000 spots were detected in a placental 2‐D gel stained with Coomassie blue. Subsequent excision of 380 spots from gels and MALDI‐TOF MS analysis allowed the identification of 273 proteins. Our results revealed the composite profiles of key proteins in the bovine placenta during late pregnancy. These protein profiles will shed light on placental function during pregnancy and assist with functional analysis of the proteins.
Two decades of responses (1986–2006) to climate by the Laurichard rock glacier, French Alps
Authors: Bodin Xavier , Thibert Emmanuel , Fabre Denis , Ribolini Adriano , Schoeneich Philippe , Francou Bernard , Reynaud Louis , Fort Monique 
Published: November 2009

AbstractThe Laurichard active rock glacier is the permafrost‐related landform with the longest record of monitoring in France, including an annual geodetic survey, repeated geoelectrical campaigns from 1979 onwards and continuous recording of ground temperature since 2003. These data were used to examine changes in creep rates and internal structure from 1986 to 2006. The control that climatic variables exert on rock glacier kinematics was investigated over three time scales. Between the 1980s and the early 2000s, the main observed changes were a general increase in surface velocity and a decrease in internal resistivity. At a multi‐year scale, the high correlation between surface movement and snow thickness in the preceding December appears to confirm the importance of snow cover conditions in early winter through their influence on the ground thermal regime. A comparison of surface velocities, regional climatic datasets and ground sub‐surface temperatures over six years suggests a strong relation between rock glacier deformation and ground temperature, as well as a role for liquid water due to melt of thick snow cover. Finally, unusual surface lowering that accompanied peak velocities in 2004 may be due to a general thaw of the top of the permafrost, probably caused both by two successive snowy winters and by high energy inputs during the warm summer of 2003. Copyright © 2009 John Wiley & Sons, Ltd.
Modern tundra landscapes of the Kolyma Lowland and their evolution in the Holocene
Authors: Veremeeva Alexandra , Gubin Stanislav 
Published: November 2009

AbstractThe different tundra landscapes of the Kolyma Lowland, northern Siberia are described, focusing on landscape typology, digital mapping and the calculation of areas occupied by different landscape complexes. The genesis, age and evolution of ‘yedoma’ sediments and associated alas (thermokarst basins) topography are inferred from the calculation of lake‐basin and lake‐area changes over three decades. Connections between the present landscape and its evolution during the Holocene are described. Copyright © 2009 John Wiley & Sons, Ltd.
Terpenoid content of Valeriana wallichii extracts and antidepressant‐like response profiles
Authors: Subhan Fazal , Karim Nasiara , Gilani Anwarul Hassan , Sewell Robert D. E. 
Published: November 2009

AbstractThree extracts of Valeriana wallichii DC (Valerianaceae) rhizome and fluoxetine were studied for antidepressant‐like activity in two behavioral models, namely the forced swim test (FST) and the tail suspension test (TST). Fluoxetine as well as methanolic and aqueous extracts of V. wallichii induced monophasic dose‐related decrements in immobility times in both tests. However, the aqueous‐ethanolic fraction induced a biphasic dose‐response profile since it produced a graded effect up to 200 mg/kg but the highest dose (250 mg/kg) was inactive in the FST. This extract also exhibited significantly reduced activity at 200 mg/kg compared to lower doses in the TST. The highest doses of aqueous‐ethanolic extract also reduced locomotor activity which will have led to a negative functional interaction with antidepressant‐like effects. Qualitative phytochemical analysis revealed that the aqueous‐ethanolic extract of V. wallichii was the only separated rhizome fraction containing terpenoids. Furthermore, since the methanolic and aqueous extracts were active in the tests, it is suggested that the antidepressant‐like action of this herbal plant is not contingent upon its terpenoid constituents. Copyright © 2009 John Wiley & Sons, Ltd.
Acetylcholinesterase inhibitory potential of a carbazole alkaloid, mahanimbine, from Murraya koenigii
Authors: Kumar N. Satheesh , Mukherjee Pulok K. , Bhadra S. , Saha B. P. , Pal B. C. 
Published: November 2009

AbstractIn the search for acetylcholinesterase (AChE) inhibitors from Indian medicinal plants, via bioassay‐guided isolation, a carbazole alkaloid, mahanimbine [3, 5‐dimethyl‐3‐(4‐ methylpent‐3‐enyl)‐11H‐pyrano [5, 6‐a] carbazole], was isolated from the petroleum ether extract of the leaves of Murraya koenigii. Inhibition of AChE was evaluated based on Ellman's method using 96‐well microplate readers. Mahanimbine inhibited AChE activity in a dose‐dependent manner with an IC50 value of 0.03 ± 0.09 mg/mL, while galantamine was used as a standard. The AChE inhibitory activity of this carbazole alkaloid has not been reported so far, and this study is the first to reveal this activity in carbazole alkaloid mahanimbine, isolated from Murraya koenigii. Copyright © 2009 John Wiley & Sons, Ltd.
Caffeic acid improves the bioavailability of l‐dopa in rabbit plasma
Authors: Wang Li‐Hsuan , Hsu Kuang‐Yang , Uang Yow‐Shieng , Hsu Feng‐Lin , Yang Li‐Ming , Lin Shwu‐Jiuan 
Published: November 2009

AbstractThe impacts of caffeic acid (3,4‐dihydroxycinnamic acid, CA) on the pharmacokinetics of levodopa (L‐dopa) were studied in rabbits. A single dose of 5/1.25 mg·kg−1 l‐dopa/carbidopa was administered alone or was co‐administered with three different doses of caffeic acid (2.5, 5, and 10 mg·kg−1), or a single dose of 5 mg·kg−1 caffeic acid was administered alone via an intramuscular route to six rabbits each in a crossover treatment protocol. Plasma levels of l‐dopa, 3‐O‐methyldopa (3‐OMD), caffeic acid, and ferulic acid were determined and subsequently used to calculate their pharmacokinetic parameters. The results indicated that caffeic acid administered at a dose of 10 mg·kg−1 decreased about 22% of the peripheral formation of 3‐OMD and about 31% of the Cmax of 3‐OMD. In addition, the metabolic ratios (MR, AUC of 3‐OMD/AUC of L‐dopa) decreased by about 22%. Results also indicated that caffeic acid significantly decreased the proportion of 3‐OMD (p < 0.05). In contrast, the parameters of neither caffeic acid nor ferulic acid were significantly affected by l‐dopa/carbidopa. In conclusion, caffeic acid at a dose of 10 mg·kg−1 can significantly affect the COMT metabolic pathway of L‐dopa. Copyright © 2009 John Wiley & Sons, Ltd.
Angiogenesis inhibition by a lichen compound olivetoric acid
Authors: Koparal A. Tansu , Ulus Gönül , Zeytinoğlu Melih , Tay Turgay , Türk Ayşen Özdemir 
Published: November 2009

AbstractLichens have been used in folk medicine for centuries and are symbiotic organisms of fungi and algae that produce unique secondary metabolites. Olivetoric acid is one of these secondary metabolites. In the present study, the effect of olivetoric acid isolated from acetone extract of the lichenPseudevernia furfuracea(var.ceratea) on angiogenesis was evaluated. It displayed potent anti‐angiogenic activitiesin vitro: inhibited proliferation of rat adipose tissue endothelial cells (RATECs) and disrupted endothelial tube formation in a dose‐dependent manner. Furthermore, dose‐dependent depolymerization effects of olivetoric acid on F‐actin stress fibers were observed. Decrease in the tube formation of RATECs by olivetoric acid might be explained by a disorganization of the actin cytoskeleton. These findings suggest that olivetoric acid is a new anti‐angiogenic agent and can be developed as a new therapeutic agent for angiogenesis‐related diseases. Copyright © 2009 John Wiley & Sons, Ltd.
Antioxidant and scavenging capacity of Anacamptis pyrimidalis L. – Pyrimidal Orchid from Vojvodina
Authors: Štajner Dubravka , Popović Boris M. , Kapor Agneš , Boža Pal , Štajner Marijana 
Published: November 2009

AbstractIn the present study we investigated the antioxidative properties of flowers and aboveground part of Anacamptis pyrimidalis L. from Vojvodina. Activities of antioxidant enzymes (superoxide dismutase, catalase, peroxidase, and glutathione peroxidase), quantities of malonyldialdehyde, superoxide and hydroxyl radicals and reduced glutathione and also the contents of chlorophylls a and b, carotenoids and soluble proteins were determined. Lipofuscin ‘plant age pigments’, total antioxidant capacity and scavenging activity were also determined. Our results indicated that the aboveground part of the plant exhibited higher antioxidant activity due to low MDA and lipofuscin pigment accumulation (2.82 nmol/mg protein; 92.90%), higher scavenging activity (54.16%) and antioxidant capacity (432.00 μmol Fe2+/dm3). Copyright © 2009 John Wiley & Sons, Ltd.
Role of ethanol in kava hepatotoxicity
Authors: Li X. Z. , Ramzan I. 
Published: November 2009

AbstractKava is known for its recreational, ceremonial and medicinal use in the Pacific. The aqueous non‐alcoholic drink of kava rhizome produces intoxicating, relaxing and soothing effects. While kava's medicinal effects receive worldwide recognition, kava‐containing products came under scrutiny after over 100 reports of spontaneous adverse hepatic effects. Many mechanisms have been postulated to explain the unexpected toxicity, one being pharmacokinetic interactions between kavalactones and co‐administered drugs involving cytochrome P450 enzyme system. Alcohol is often co‐injested in kava hepatotoxicity cases. This review evaluates the possible hepatotoxicity mechanisms involving alcohol and kava. Copyright © 2009 John Wiley & Sons, Ltd.
Fault detection for output feedback control systems with actuator stuck faults: A steady‐state‐based approach
Authors: Yang Guang‐Hong , Wang Heng , Xie Lihua 
Published: November 2009

AbstractThe paper studies the fault detection problem for output feedback control systems with bounded disturbances and nonzero constant reference inputs. A steady‐state‐based approach is proposed which can be used to detect small actuator stuck faults including actuator outage (the stuck value is zero). These small stuck faults, especially the outage faults, cannot be detected effectively using the existing techniques. A dynamic output feedback controller and a weighting matrix are designed simultaneously. The dynamic output feedback controller stabilizes the closed‐loop system for both fault‐free and faulty cases and attenuates the effects of disturbances. By manipulating the steady‐state values of system states with the detection weighting matrix, a residual is then generated, through which actuator stuck faults including actuator outages can be detected effectively. Simulation results are included to demonstrate our design procedure. Copyright © 2009 John Wiley & Sons, Ltd.
Joint modeling of longitudinal ordinal data and competing risks survival times and analysis of the NINDS rt‐PA stroke trial
Authors: Li Ning , Elashoff Robert M. , Li Gang , Saver Jeffrey 
Published: November 2009

AbstractExisting joint models for longitudinal and survival data are not applicable for longitudinal ordinal outcomes with possible non‐ignorable missing values caused by multiple reasons. We propose a joint model for longitudinal ordinal measurements and competing risks failure time data, in which a partial proportional odds model for the longitudinal ordinal outcome is linked to the event times by latent random variables. At the survival endpoint, our model adopts the competing risks framework to model multiple failure types at the same time. The partial proportional odds model, as an extension of the popular proportional odds model for ordinal outcomes, is more flexible and at the same time provides a tool to test the proportional odds assumption. We use a likelihood approach and derive an EM algorithm to obtain the maximum likelihood estimates of the parameters. We further show that all the parameters at the survival endpoint are identifiable from the data. Our joint model enables one to make inference for both the longitudinal ordinal outcome and the failure times simultaneously. In addition, the inference at the longitudinal endpoint is adjusted for possible non‐ignorable missing data caused by the failure times. We apply the method to the NINDS rt‐PA stroke trial. Our study considers the modified Rankin Scale only. Other ordinal outcomes in the trial, such as the Barthel and Glasgow scales, can be treated in the same way. Copyright © 2009 John Wiley & Sons, Ltd.
The influence of non‐structural components on tall building stiffness
Authors: Li Bing , Hutchinson Graham L. , Duffield Colin F. 
Published: November 2009

AbstractThe lateral load resisting system of a multi‐storey building is considered to be an assembly of structural components, such as the structural frame, shear walls, concrete cores, etc. However, in reality, some so‐called ‘non‐structural components (NSCs)’ also play important roles in adding stiffness to the building. To evaluate the contributions from those NSCs and to quantify some of their contributions to the stiffness of the structure under service level loads, this paper reports on the analysis of a lateral load resisting system with different components so that the stiffness contribution from each individual component may be evaluated. Results from finite element analyses are verified by other theoretical calculations. Discussions and conclusions on the performance of both single components and the building system are also provided. Copyright © 2009 John Wiley & Sons, Ltd.
On delay constrained CAC scheme and scheduling policy for CBR traffic in IEEE 802.11e wireless LANs
Authors: Deng Der‐Jiunn , Ke Chih‐Heng , Chao Han‐Chien , Huang Yueh‐Min 
Published: November 2009

AbstractCBR (constant bit rate) traffic is expected to be an important traffic source in wireless networks. Such sources usually have stringent jitter or delay requirements and in many cases they should be delivered exactly as they were generated. In this paper, we propose a strictly guaranteed QoS (quality‐of‐service) provisioning CAC (call admission control) scheme with a polling‐based scheduling policy for CBR traffic in IEEE 802.11e wireless LANs. Under such a scheme, the proposed transmit‐permission policy for HCCA (HCF controlled channel access) method can forecast the maximum suffered delay for each packet and derive sufficient conditions so that all the CBR sources satisfy their time constraints to provide deterministic QoS guarantees. A simple analytical model is carried out to estimate the average queueing delay of the proposed scheme. In addition to theoretical analysis, simulations are conducted to validate its promising performance. Our simulation results show that the proposed scheme maintains a high throughput with respect to the whole range of system load. Copyright © 2009 John Wiley & Sons, Ltd.
Method and portable facility for energy‐dispersive X‐ray fluorescent analysis of zinc content in needle‐biopsy specimens of prostate
Authors: Zaichick Sofia , Zaichick Vladimir 
Published: November 2009

AbstractA facility and method for radionuclide‐induced (109Cd) energy‐dispersive X‐ray fluorescent (EDXRF) were developed to determine the Zn content in the needle‐biopsy specimens of human prostate. The material received from 33 subjects was used for quality control of Zn results and estimation of Br, Rb, Sr, and Zn contents in prostate: 23 patients (13 benign prostatic hyperplasia(BPH)and 10 cancer) and 10 apparently healthy persons (control group of men died unexpectedly). All materials were divided into two parts. One of them was morphologically examined while Zn content of another one was estimated. Mean values (M ± SEM) for mass fraction of Zn (mg kg−1 on dry weight basis) in normal, hyperplastic and cancerous prostate tissues were 1110 ± 140, 1140 ± 110, and 134 ± 18, respectively. It was shown that the needle‐biopsy specimens were sufficiently representative for an assessment of the Zn content in affected tissue of prostate. The developed method provides Zn estimation in both fresh and dry needle‐biopsy specimens, as well as in samples made of lyophilized and homogenized tissues of resected prostate. The facility for EDXRF is comparatively compact and can be located in close proximity to the site of carrying out the biopsy procedure. Additionally, the developed facility and method allow to determine Br, Rb, and Sr contents in samples of prostate tissue. Copyright © 2009 John Wiley & Sons, Ltd.
A Multi-feature Reproducibility Assessment of Mass Spectral Data in Clinical Proteomic Studies
Authors: Zeng Irene S. L. , Browning Sharon R. , Gladding Patrick , Jüllig Mia , Middleditch Martin , Stewart Ralph A. H. 
Published: November 2009

Abstract

Background
The use of mass spectrometry to investigate disease-associated proteins among thousands of candidates simultaneously creates challenges with the evaluation of operational and biological variation. Traditional statistical methods, which evaluate reproducibility of a single feature, are likely to provide an inadequate assessment of reproducibility. This paper proposes a systematic approach for the evaluation of the global reproducibility of multidimensional mass spectral data at the post-identification stage.

Methods
The proposed systematic approach combines dimensional reduction and permutation to test and summarize the reproducibility. First, principal component analysis is applied to the mean quantities from identified features of paired replicated samples. An eigenvalue test is used to identify the number of significant principal components which reflect the underlying correlation pattern of the multiple features. Second, a simulation-based permutation test is applied to the derived paired principal components. Third, a modified form of Bland Altman or MA plot is produced to visualize agreement between the replicates. Last, a discordance index is used to summarize the agreement.

Results
Application of this method to data from both a cardiac liquid chromatography tandem mass spectrometry experiment with iTRAQ labeling and simulation experiments derived from an ovarian cancer SELDI-MS experiment demonstrate that the proposed global reproducibility test is sensitive to the simulated systematic bias when the sample size is above 15. The two proposed test statistics (max t statistics and a sign score statistic) for the permutation tests are shown to be reliable.

Conclusion
The methodology presented in this paper provides a systematic approach for the global measurement of reproducibility in clinical proteomic studies.
EU DRUID project: results of a questionnaire survey amongst participants of driver rehabilitation programmes in Europe
Authors: Klipp Simone 
Published: November 2009

AbstractPurposeThis study was one part of the research activities of work package 5 “Rehabilitation” of the integrated EU DRUID project (6th Framework Programme). It aimed at gathering information about the cognitive–affective and behavioural processes that participants undergo while attending driver rehabilitation (DR) programmes. The primary objective was to analyse the outcomes of group interventions for alcohol offenders in order to assess any cognitive, motivational and behavioural modifications within individual participants and to identify the relevant variables which initiate and support this change process.MethodsThe general methodological concept of the study was a prospective cohort design of participants of group-based European driver rehabilitation programmes, carried out via a participant feedback questionnaire survey. In totalN = 7.339 DUI offenders in 9 European countries (Austria, Belgium, France, Germany, Great Britain, Hungary, Italy, Netherlands and Poland) participated in the survey.ResultsThe results indicate that DR participants feel such programmes provide strong support for their cognitive and behavioural change processes. The findings suggest that participants feel encouraged to establish new behavioural goals and the commitment to stick to them. At the same time, the participants’ ratings emphasise the important role of the course leader in encouraging such changes.ConclusionsThe findings of this explorative questionnaire survey are promising. Although it is impossible to draw any conclusions regarding long-term behavioural changes or effects on recidivism rates, participants of DR courses express positive feedback on completion of the programme. The positive outcomes of the study can motivate decision-makers to launch DR measures and to regard them as an essential part of a comprehensive countermeasure system against DUI.
Roles of kinin B1 and B2 receptors in skin cancer pain produced by orthotopic melanoma inoculation in mice
Authors: Fujita Masahide , Andoh Tsugunobu , Ohashi Kazuto , Akira Ahmad , Saiki Ikuo , Kuraishi Yasushi 
Published: November 2009

AbstractBackground: Although bradykinin is a potent algogenic peptide, the roles of this peptide and kinin receptors in cancer pain are unclear.Aims: The present study was conducted to clarify whether kinin B1 and B2 receptors would be involved in pain using a mouse model of skin cancer pain.Methods: B16‐BL6 melanoma cells were inoculated into the hind paw of C57BL/6 mice. Licking, an index of spontaneous pain, allodynia and hyperalgesia were observed. Expression of kinin receptor mRNAs was analyzed with reverse transcription and polymerase chain reaction. The contents of kininogen and bradykinin‐related peptides were assayed with Western blotting and enzyme immunoassay, respectively.Results: Melanoma inoculation induced spontaneous licking of the melanoma‐bearing paw from day 18 post‐inoculation, which was inhibited by local injections of B1 and B2 receptor antagonists. Allodynia was briefly attenuated by B2, but not B1 antagonist and hyperalgesia was not inhibited by either B1 or B2 antagonist. Local injections of B1 and B2 receptor agonists increased licking behavior in melanoma‐bearing, but not healthy, paw. The expression of kinin B1, but not B2, receptor mRNA was markedly increased in the L4/5 dorsal root ganglia on the melanoma‐bearing side. Melanoma cells expressed B1 and B2 receptors and kininogen. The content of bradykinin and related peptides was increased in the melanoma mass as compared with healthy skin.Conclusions: Bradykinin and related peptides released from melanoma cells may cause spontaneous pain and allodynia in the melanoma‐bearing paw, in which B1 and B2 receptors on primary afferent and melanoma cells may have different roles.