bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cell cycle‐related kinase supports ovarian carcinoma cell proliferation via regulation of cyclin D1 and is a predictor of outcome in patients with ovarian carcinoma
Authors: Wu Guo‐Qing , Xie Dan , Yang Guo‐Feng , Liao Yi‐Ji , Mai Shi‐Juan , Deng Hai‐Xia , Sze Johnny , Guan Xin‐Yuan , Zeng Yi‐Xin , Lin Marie C. , Kung Hsiang‐Fu 
Published: June 2009

AbstractOur previous study has suggested that the cell cycle‐related kinase (CCRK) is a putative candidate oncogene in glioblastoma tumorigenesis. The potential oncogenic role of CCRK and its clinical/prognostic significance, however, in ovarian carcinoma are unclear. In this study, CCRK expression was examined by immunohistochemistry in a series of ovarian carcinoma tissues. Overexpression of CCRK was detected in 53% of the ovarian carcinomas, and it was positively correlated with an ascending histological grade and/or advanced clinical stage of the disease (p < 0.05). In addition, overexpression of CCRK in ovarian carcinoma was determined to be a strong and an independent predictor of short overall survival (p < 0.05). In ovarian carcinoma cells, CCRK knockdown by RNAi led to a G1 phase cell cycle arrest, while CCRK overexpression by stable transfection of CCRK‐containing plasmid pcDNA‐CCRK promoted cell proliferation in vitro and tumor growth in vivo. In addition, CCRK knockdown was found to reduce cyclin D1 expression. Consistently, CCRK overexpression increased cyclin D1 expression, and furthermore, a significant correlation between expression of CCRK and cyclin D1 in ovarian carcinomas was observed (p < 0.001). These findings suggest a potential important role of CCRK in the control of cell proliferation via regulation of cyclin D1 expression, and the overexpression of CCRK, as detected by immunohistochemistry, is an independent molecular marker for shortened survival time of patients with ovarian carcinoma. © 2009 UICC
A multi‐agent‐based hybrid framework for international marketing planning under uncertainty
Authors: Li Shuliang , Li Jim Zheng 
Published: June 2009

AbstractThe increased complexity and competition in the global marketing environment present new challenges to decision‐makers. The characteristics of the international marketing planning problem are clarified in this paper. The advantages and disadvantages of relevant techniques and technologies that may be applied to deal with the planning problem are analysed. A multi‐agent‐based hybrid intelligent framework for international marketing planning and associated Internet strategy formulation is then established, with underlying techniques, technologies, software architecture and integration method outlined. In addition, a software prototype of the hybrid framework, called AgentsInternational, is created and presented, with initial evaluation results reported. Further work on this topic is also planned. Copyright © 2009 John Wiley & Sons, Ltd.
The effects of technology shocks on hours and output: a robustness analysis
Authors: Canova Fabio , Lopez‐Salido David , Michelacci Claudio 
Published: June 2009

AbstractWe analyze the effects of neutral and investment‐specific technology shocks on hours and output. Long cycles in hours are removed in a variety of ways. Hours robustly fall in response to neutral shocks and robustly increase in response to investment‐specific shocks. The percentage of the variance of hours (output) explained by neutral shocks is small (large); the opposite is true for investment‐specific shocks. ‘News shocks’ are uncorrelated with the estimated technology shocks. Copyright © 2009 John Wiley & Sons, Ltd.
Prevention of pin tract infection with titanium‐copper alloys
Authors: Shirai Toshiharu , Tsuchiya Hiroyuki , Shimizu Tohru , Ohtani Kaori , Zen Yo , Tomita Katsuro 
Published: June 2009

AbstractThe most frequent complication in external fixation is pin tract infection. To reduce the incidence of implant‐associated infection, many published reports have looked at preventing bacterial adhesion by treating the pin surface. This study aimed to evaluate the antibacterial activity of a Titanium‐Copper (Ti‐Cu) alloy on implant infection, and to determine the potential use of the Ti‐Cu alloy as a biomaterial. Two forms of Ti‐Cu alloys were synthesized: one with 1% Cu and the other with 5% Cu. For analyzing infectious behavior, the implants were exposed to Staphylococcus aureus and Escherichia coli. The reaction of pathogens to the Ti‐Cu alloys was compared with their reaction to stainless steel and pure titanium as controls. Both Ti‐Cu alloys evidently inhibited colonization by both bacteria. Conversely, cytocompatibility studies were performed using fibroblasts and colony formation on the metals was assessed by counting the number of colonies. Ti‐1% Cu alloy showed no difference in the number of colonies compared with the control. External fixator pins made of Ti‐Cu alloys were evaluated in a rabbit model. The tissue‐implant interactions were analyzed for the presence of infection, inflammatory changes and osteoid‐formation. Ti‐1% Cu alloy significantly inhibited inflammation and infection, and had excellent osteoid‐formation. Copper blood levels were measured before surgery and at 14 days postoperatively. Preoperative and postoperative blood copper values were not statistically different. Overall, it was concluded that Ti‐Cu alloys have antimicrobial activity and substantially reduce the incidence of pin tract infection. Ti‐1% Cu alloy shows particular promise as a biomaterial. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
Fabrication of a biodegradable polysaccharide hydrogel with riboflavin, vitamin B2, as a photo‐initiator and L‐arginine as coinitiator upon UV irradiation
Authors: Kim Sin‐Hee , Chu Chih‐Chang 
Published: June 2009

AbstractThe objective of this study is to develop a completely natural material based photo‐initiation system for fabricating biologically compatible hydrogels, that is, free of any synthetic materials like conventional synthetic photo‐initiators. The resulting biodegradable hydrogels would be completely free of conventional toxic synthetic substances. Dextran‐based precursor was used as a model system for this study. The natural material based photo‐initiation system used riboflavin (vitamin B2) as photo‐initiator and L‐arginine as a coinitiator. Dextran‐methacrylate precursor was prepared to introduce photosensitive unsaturated moieties into dextran for subsequent photo‐induced gel formation. Riboflavin alone could not initiate the photo‐crosslinking reaction of the dextran methacrylate solution upon UV irradiation. L‐arginine, which can act as an electron donor in the photo‐initiation process, was used as a coinitiator. The optimum gel formulation conditions in terms of the concentrations of riboflavin and L‐arginine as well as different pH media were extensively investigated. The dextran‐methacrylate hydrogels were formed well and fast at low riboflavin concentration range (0.2–1 wt %), and L‐arginine promoted the photo‐initiation at all concentrations. The gelation was promoted most in the neutral pH and the least in the alkaline pH. The turbidity of the dextran‐methacrylate hydrogel precursor solution affected the polymerization because UV penetration was significantly hindered as the opacity of the solution increased. The swelling property and structural visualization of the prepared hydrogels were also investigated. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
Effects of e‐beam radiation, storage, and hydration on osteoinductivity of DBM/AM composite
Authors: Qiu Qing‐Qing , Liu Xiang‐Hong , Connor Jerome 
Published: June 2009

AbstractE‐beam irradiation is often used to sterilize medical devices including demineralized bone matrix (DBM) products. In this study, the effect of e‐beam on osteoinductivity of a DBM product in hydrous and anhydrous configurations has been evaluated at 0‐, 6‐ and 12‐month ambient storage using a nude rat muscle pouch model. The thermal and structural stabilities of DBM and acellular dermal matrix (AM) composites were analyzed using differential scanning calorimetry (DSC) and trypsin digestion assay. Both hydrous and anhydrous DBM/AM composites exhibited osteoinductivity after e‐beam irradiation of 15 kGy. After 12‐month ambient storage, the osteoinductivity of hydrous DBM/AM was diminished, whereas the anhydrous DBM/AM retained its osteoinductive potential. However, the DSC and trypsin analysis revealed that the DBM in anhydrous DBM/AM was more vulnerable to damage from e‐beam irradiation than its hydrous counterpart. This study has found that although the anhydrous DBM has more structural damage than hydrous DBM from e‐beam irradiation, it has retained its osteoinductivity better after 1‐year ambient storage. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
Comparison of periprosthetic tissue digestion methods for ultra‐high molecular weight polyethylene wear debris extraction
Authors: Baxter Ryan M. , Steinbeck Marla J. , Tipper Joanne L. , Parvizi Javad , Marcolongo Michele , Kurtz Steve M. 
Published: June 2009

AbstractThere is considerable interest in characterization of wear debris from polyethylene (UHMWPE) bearing components used in total joint replacement. To isolate UHMWPE wear debris, tissue samples must be excised from regions adjacent to revised UHMWPE implant components, followed by exposure to one of many available tissue digestion methods. Numerous studies demonstrate successful digestion, but the relative efficiency of each method is not clear. The purpose of this study was to evaluate a variety of conditions for tissue digestion to provide a quantitative comparison of methods. Porcine and human hip tissues were exposed for 24 h to basic, acidic or enzymatic agents, filtered and digestion efficiency calculated based on the percentage of initial to final tissue weight. Of the conditions tested, 5 M NaOH, 5 M KOH, 15 M KOH or 15.8 M HNO3 yielded the most complete porcine hip tissue digestion (<1% residual tissue weight; p < 0.05). Proteinase K and Liberase Blendzyme 3 did not effectively digest tissue in a 24 h period. Similar to results from the porcine dataset, human tissues digestion was most efficient using 5 M NaOH, 5 M KOH or 15.8 M HNO3 (<1% residual tissue weight; p < 0.05). To verify that particle surface modifications did not occur after prolonged reagent exposure, GUR415 and Ceridust 3715 particles were immersed in each solution for 24 h. Overall, this study provides a framework for thorough and efficient digestive methods for UHMWPE wear debris extraction. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
Comparative evaluation of different crystal‐structured calcium sulfates as bone‐filling materials
Authors: Woo Kyung Mi , Yu Bin , Jung Hong‐Moon , Lee Yong‐Keun 
Published: June 2009

AbstractThe mechanical and handling properties and biological performances of two types of calcium sulfate (βCS and αCS) as bone‐filling materials were compared. The influence of two modifiers such as hydroxypropylmethylcellose (HPMC) and fibrin was also examined. αCS showed higher strength than, and similar setting time and injectability to those of βCS. The degradation of CS in a simulated body fluid (SBF) was checked by measuring the amount of calcium released to SBF. αCS showed reduced calcium release than βCS. The modifiers tended to increase the calcium release. The MC3T3‐E1 preosteoblasts cultured on αCS showed higher levels of alkaline phosphatase (ALP) activity than those cultured on βCS. αCS strongly promoted gene expression of osteoblast phenotypes including Runx2, α1(I) collagen, osteocalcin, and bone sialoprotein. There was no significant difference in cell adhesion and proliferation between two types of CS. The addition of modifiers to CS increased cell proliferation, ALP activity, and the gene expression. The osteoclastic differentiation of RAW264.7 monocytes was checked. The cells on both types of CS produced tartrate‐resistant acid phosphatase (TRAP) activity with no significant difference. These cell response results indicated that αCS promoted osteoblast differentiation over βCS but not osteoclast differentiation. Conclusively, a particular form of commercially available αCS possesses superior properties to βCS in terms of mechanical properties and supports for osteoblast differentiation, suggesting that αCS could be an alternative to the conventionally used βCS. The addition of HPMC and fibrin could further improve the feasibility of αCS as a bone‐filling material. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2009
Tumor liberated protein from lung cancer and perspectives for immunotherapy
Authors: Tarro Giulio 
Published: June 2009

AbstractThis review addresses peptide regions of human tumor liberated protein (TLP) complexes with antigenic activity. The review also deals with antibodies reacting with such proteins, to be used for diagnostic and clinical purposes. We identified peptide sequences of the TLP 100 kDa protein. Lung cancer cells were found positive with an immuno‐histochemical test, by peroxidase‐anti‐peroxidase, using a specific antiserum. This test turned out negative in the presence of the synthesized peptide (blocking test). Several tumors were used to obtain antigens by various purification procedures (cell homogenization, ultracentrifugation at 100,000g, gel filtration and chromatography, preparative and analytical isoelectric focusing, IEF). The molecular weight on SDS–PAGE of an immunogenic peak detected on IEF was determined to be a value of 214,000 Da, with monomers of 54,000 Da. In vivo TLP yields delayed hypersensitivity reactions in both autologous and homologous hosts, and does not seem to have any nonspecific mytogenic activity, but does have mytogenic‐specific activity since lymphocyte blastogenesis (TLP‐induced in pre‐sensitized patients), in the case of intradermal inoculation, could mean its ability to sensitize a lymphocytic clone. J. Cell. Physiol. 221: 26–30, 2009. © 2009 Wiley‐Liss, Inc
Explanation for missing limbs in deformed amphibians
Authors: Ballengée Brandon , Sessions Stanley K. 
Published: June 2009

AbstractWe present evidence that the most commonly found deformities in wild‐caught amphibians, those featuring missing limbs and missing limb segments, may be the result of selective predation. Here we report that predatory dragonfly nymphs can severely injure and even fully amputate developing hind limbs of anuran tadpoles. Developmental responses of the injured/amputated tadpole limbs range from complete regeneration to no regeneration, with intermediate conditions represented by various idiosyncratic limb deformities, depending mainly on the developmental stage of the tadpole at the time of injury/amputation. These findings were reinforced by experimental amputations of anuran tadpole hind limbs that resulted in similar deformities. Our studies suggest that selective predation by dragonfly nymphs and other aquatic predators may play a significant role in the most common kinds of limb deformities found in natural populations of amphibians.J. Exp. Zool. (Mol. Dev. Evol.) 312B:770–779, 2009. © 2009 Wiley‐Liss, Inc.
Acute vertebral fracture
Authors: Agulnek Abby N. , O'Leary Kevin J. , Edwards Beatrice J. 
Published: June 2009

AbstractVertebral fractures resulting from osteoporosis are a significant cause of morbidity and mortality in the aging population and are commonly seen in the hospital setting. Appropriately assessing and treating these conditions continues to be a challenge. This article is an evidence‐based clinical update on the evaluation and management options of acute vertebral fractures, ranging from conservative treatment to surgical intervention. Hospitalists can play an influential role in the management of osteoporosis. Journal of Hospital Medicine 2009;4:E20–E24. © 2009 Society of Hospital Medicine.
The combined effect of superchilling and modified atmosphere packaging using CO2 emitter on quality during chilled storage of pre‐rigor salmon fillets (Salmo salar)
Authors: Hansen Anlaug Ådland , Mørkøre Turid , Rudi Knut , Langsrud Øyvind , Eie Thomas 
Published: June 2009

AbstractBACKGROUND: Pre‐rigor fillets of Atlantic salmon were either superchilled or chilled prior to packaging in air or in modified atmosphere (MAP, 60% CO2/40% N2) with a CO2 emitter, in 5.3 L high‐density polyethylene trays with three to four layers of fillets (3.0–3.7 kg). All samples were stored at 0.1 °C for 28 days.RESULTS: Fillets stored in MAP had significantly lower bacterial growth compared to fillets stored in air, and MAP superchilled bottom fillets had lower bacterial counts compared to the corresponding chilled fillets. Samples superchilled prior to refrigerated storage in air had similar bacterial growth to ordinary chilled samples. Faster fillet softening during storage and higher liquid loss were observed in superchilled MAP samples.CONCLUSION: Combining short‐term superchilling and MAP with a CO2 emitter prolonged the shelf‐life of pre‐rigor salmon fillets, which can improve sustainability throughout the value chain. The superchilling method needs to be optimized to avoid negative effects on texture and liquid loss. Copyright © 2009 Society of Chemical Industry
Alternate wetting and moderate soil drying increases grain yield and reduces cadmium accumulation in rice grains
Authors: Yang Jianchang , Huang Dongfen , Duan Hua , Tan Guilu , Zhang Jianhua 
Published: June 2009

AbstractBACKGROUND: Rice is the most important staple food in Asia but has also been identified as one of the major sources of cadmium (Cd) intakes for some Asian population. This study investigated whether grain yield could be maintained but Cd in grains be reduced through proper irrigation management when rice was grown in Cd‐contaminated soil.RESULTS: Compared to the well watered treatment, the alternate wetting and moderate soil drying (MD, re‐watered when soil water potential decreased to −20 kPa) increased grain yield by 10–12% and improved milling and appearance quality of rice when grown in a soil containing a water‐soluble Cd content of 18 g kg−1. An alternate wetting and severe soil drying (SD, re‐watered when soil water potential decreased to −40 kPa) showed an opposite effect. Both MD and SD significantly increased Cd content in roots while they reduced it in the straw. MD reduced Cd content by 19–21% in the grain and by 40% in milled rice. The SD significantly increased Cd content in the grain but reduced it in milled rice.CONCLUSION: An alternate wetting and moderate soil drying could increase rice yield and quality and also reduce Cd in the diet of rice. Copyright © 2009 Society of Chemical Industry
Discrimination of climacteric and non‐climacteric melon fruit at harvest or at the senescence stage by quality traits
Authors: Obando‐Ulloa Javier M , Jowkar Mohammad‐Mahdi , Moreno Eduard , Souri M Kazem , Martínez Juan A , Bueso María C , Monforte Antonio J , Fernández‐Trujillo J Pablo 
Published: June 2009

AbstractBACKGROUND: This paper characterizes the quality traits at harvest and the changes associated with fruit senescence based on fruit physiological behaviour (climacteric or non‐climacteric) found in a collection of near‐isogenic lines (NILs) of melon (Cucumis melo L.). Data from both stages of postharvest development were analyzed by univariate and multivariate statistical analysis.RESULTS: The principal components and random forest analyses of the fruit quality traits allowed the best classification of the NILs by time (harvest, senescence), or by climacteric behaviour at harvest, but not at the senescent stage. The overall quality profile of the non‐climacteric senescent melons was, in general, very different from that of the climacteric ones, and was in accord with a longer storage life. Most of the taste quality traits (individual sugars or sucrose equivalents, titratable acidity and the citric, oxalacetic, glutamic and succinic acids) and the traits related to skin, flesh and juice colour parameters (chroma, hue angle) helped to distinguish the climacteric NILs from the non‐climacteric ones independently of the time considered.CONCLUSIONS: The time had a stronger effect on quality than the physiological behaviour. The discrimination by climacteric or non‐climacteric behaviour was usually better at harvest than at the senescent stage irrespective of the methodology used. Principal component analysis was the best multivariate method to discriminate by time and physiological behaviour followed by random forest and linear discriminant analysis. Copyright © 2009 Society of Chemical Industry
Thyroid gland and carotid artery anomalies in 22q11.2 deletion syndromes
Authors: de Almeida John R. , James Adrian L. , Papsin Blake C. , Weksburg Rosanna , Clark Howard , Blaser Susan 
Published: June 2009

AbstractObjectives/Hypothesis:Chromosome 22q11.2 deletion syndromes (22q11DS), such as velocardiofacial syndrome and DiGeorge syndrome demonstrate developmental anomalies affecting the branchial arch structures. Mouse models have shown abnormalities of both the thyroid gland and the common carotid arteries and have suggested a developmental link between the two structures. Here we examine radiological abnormalities of the carotid artery bifurcation and thyroid gland.Study Design:Retrospective case control study.Methods:We reviewed multiplanar contrast‐enhanced computed tomography scans with 3‐D reformats of 66 children falling into three groups: 1) children with velopharyngeal insufficiency testing positive for 22q11DS (n = 28), 2) children with velopharyngeal insufficiency testing negative for 22q11DS (n = 10), and 3) age and gender matched controls (n = 28). The common carotid bifurcations were measured according to cervical spinal (C‐spine) level. The thyroid gland morphology was also assessed.Results:The 22q11DS group demonstrated lower overall common carotid bifurcations. The median C‐spine level of bifurcation was significantly different in the 22q11DS group compared to controls on the right (5 vs. 3.;P< .01) and left (6.5 vs. 3.25;P< .001). These patients demonstrated asymmetry when comparing the bifurcations between sides. Fourteen of 28 patients (50%) with 22q11 deletions demonstrated at least one of four different thyroid anomalies. Anomalies included absence of a lobe, absence of the isthmus, retrocarotid, or retroesophageal extension. The 22q11DS group had significantly higher proportions of thyroid abnormalities compared to the control groups (P < .01).Conclusions:Patients with 22q11DS demonstrate statistically significantly lower carotid artery bifurcations and a higher proportion of radiological thyroid abnormalities. Laryngoscope, 2009