bluuebunny/crossref_metadata_2025_split huggingface dataset:
Occupational Exposures to Solar Ultraviolet Radiation of Vineyard Workers in Tuscany (Italy)
Authors: Siani Anna Maria , Casale Giuseppe R. , Sisto Renata , Colosimo Alfredo , Lang Carolyn A. , Kimlin Michael G. 
Published: April 2011

AbstractA study to quantify the UV exposure of vineyard workers was carried out using polysulphone dosimetry. The study took place in Tuscany (Italy) involving 32 vineyard workers, covering three different stages of the vine’s growth. The level of personal exposure expressed as a function on the available ambient UV radiation was determined. We also assessed skin reflectance parameters, pre and post exposure. It was found that during spring backs received between 53% and 87% of ambient exposure and arms between 30% and 60%. During summer, the workers received on the back between 36% and 77% of ambient exposure and between 19% and 43% of ambient exposure on the arm. The comparison with the occupational UV exposure limit showed that all subjects received UV exposures in excess of the limit. The exposure of back of neck exceeded 10 SED (assumed as a threshold level of sun‐adapted skin for Mediterranean subjects) in spring, which means that in the case of non sun‐adapted skin and without sun protection, erythema can be induced in this targeted population. The cumulative exposure was also estimated under specific assumptions of UV exposure giving values in some cases higher than previous studies.
Effects of Metal and the Phytyl Chain on Chlorophyll Derivatives: Physicochemical Evaluation for Photodynamic Inactivation of Microorganisms
Authors: Gerola Adriana P. , Santana Amanda , França Polyana B. , Tsubone Tayana M. , de Oliveira Hueder P. M. , Caetano Wilker , Kimura Elza , Hioka Noboru 
Published: April 2011

AbstractChlorophyll compounds and their derivatives containing metal or phytyl chain can be used as photosensitizer in photodynamic inactivation of microorganisms (PDI). So, the physicochemical properties and antimicrobial effect of chlorophyll derivatives were investigated: Mg‐chlorophyll (Mg‐Chl), Zn‐chlorophyll (Zn‐Chl), Zn‐chlorophyllide (Zn‐Chlde), Cu‐chlorophyll (Cu‐Chl), pheophytin (Pheo) and pheophorbide (Pheid). The photobleaching experiments showed photostability according to Cu‐Chl > Pheo ∼ Pheid ≫ Zn‐Chl ∼ Zn‐Chlde > Mg‐Chl. This order was discussed in terms of metal and the phytyl chain presences. Pheid and Zn‐Chl in aqueous Tween 80 solution exhibited highest singlet oxygen yield compared with the other derivatives. Chlorophyll derivatives (CD) with phytyl chain was limited by the self‐aggregation phenomenon at high concentrations, even in micellar systems (Tween 80 and P‐123). The antimicrobial effect of CD derivatives was investigated against Staphylococcus aureus, Escherichia coli, Candida albicans and Artemia salina. Pheid showed the best results against all organisms tested, Zn‐Chlde was an excellent bactericide in the dark and Cu‐Chl had no PDI effect. No correlation with CD uptake by microorganisms and darkness cytotoxicity was found. The physicochemical properties allied to bioassays results indicate that Mg‐Chl, Pheo, Zn‐Chl and Pheid are good candidates for PDI.
Long‐Run Performance of Chinese Initial Public Offerings: Further Evidence*
Authors: Su Chen , Bangassa Kenbata , Brookfield David 
Published: April 2011

AbstractThis study examines the long‐run performance of 936 Chinese initial public offerings (IPOs) over the period 1996 to 2005 (the post‐issue return evidence ends in June 2008). Using a number of empirical methods, including event‐time and calendar‐time approaches based on a size and industry matching firm benchmark, we find a significant long‐run overperformance using the equal‐weighted buy‐and‐hold abnormal returns, although not for the value‐weighted returns, suggesting that the performance of small‐size IPO firms is superior to that of large‐size IPO firms. The significant overperformance disappears, however, when using cumulative or calendar‐time abnormal returns. The preset study provides out‐of‐sample evidence in an emerging market context in support of Fama's (1998) argument that reported long‐run performance is sensitive to the method of analysis. Finally, based on a rich set of explanatory factors as proxies for both signaling and ex‐ante uncertainty characteristics, our results are supportive of the signaling hypothesis, but inconsistent with the divergence of opinion hypothesis.
The expanding public health threat of obesity and overweight
Authors: Fincham Jack E 
Published: April 2011

Abstract
Obesity and overweight is a continuing public health crisis. This health threat respects no boundaries or economic strata, and is a pervasive universal threat with short- and long-term negative sequelae associated. Pharmacists and other health professionals need to structure interventions from individual patient and population-based standpoints. Documentation of effects of programmes needs to be disseminated and incorporated into educational, research and practice-based efforts.
Unintentional injury deaths among children younger than 5 years of age in India: a nationally representative study
Authors: Jagnoor Jagnoor , Bassani Diego G , Keay Lisa , Ivers Rebecca Q , Thakur J S , Gururaj G , Jha Prabhat ,   for the Million Death Study Collaborators
Published: April 2011

ObjectiveTo determine the mortality burden associated with unintentional injuries among children younger than 5 years of age in India.MethodsThe Registrar General of India conducted verbal autopsy for all deaths occurring in 2001–2003 in a nationally representative sample of over 1.1 million homes. These verbal autopsy reports were coded by two of 130 trained physicians, who independently assigned an ICD-10 code to each death. Discrepancies were resolved through reconciliation and, if necessary, adjudication. The probability of death during the first 5 years of life (per 100 000 live births) was estimated from the 2005 United Nations' population and death estimates for India, to which the proportions of deaths from the mortality study were applied.ResultsUnintentional injuries were the sixth leading cause of death among children under 5 years of age. In 2005, unintentional injuries led to 82 000 deaths (99% CI 71 000 to 88 000) among children under 5 years of age, a mortality rate per 100 000 live births (MR) of 302 (99% CI 262 to 323). Mortality was higher in rural areas (MR=339, 99% CI 282 to 351), mostly due to more drowning deaths, than in urban areas (MR=173, 99% CI 120 to 237), where falls were the leading cause of child injury mortality.ConclusionUnintentional injuries, specifically drowning and falls, lead to substantial mortality in children younger than 5 years of age in India. There is a need for continued monitoring of the injury burden and investigation of risk factors for evidence-based effective injury prevention programmes.
Glypican-3 is expressed in rhabdomyosarcomas but not adult spindle cell and pleomorphic sarcomas
Authors: Thway Khin , Selfe Joanna , Missiaglia Edoardo , Fisher Cyril , Shipley Janet 
Published: April 2011

AimsGlypican-3 (GPC3) is a membrane-bound heparan sulphate proteoglycan. Glypicans are predominantly expressed during development in cell- and tissue-specific manners and act as co-receptors for many heparin-binding growth factors, playing important roles in cell growth and differentiation. GPC3 expression has been linked to several visceral malignancies, but its role in sarcomas is unexplored. Rhabdomyosarcomas (RMS), the most common paediatric soft tissue sarcomas (STS), are aggressive tumours that frequently show histological overlap with other STS. Immunohistochemistry was performed on tissue microarrays to study GPC3 expression in paediatric RMS and a variety of the more common spindle cell and pleomorphic adult sarcomas.Methods642 tumour samples encompassing alveolar and embryonal RMS and adult STS (fibrosarcoma, synovial sarcoma, leiomyosarcoma and undifferentiated pleomorphic sarcoma) were stained with anti- GPC3, and percentage expression in tumour cells was scored on a scale of 0 to +3.ResultsGPC3 immunostaining was positive (+1 to +3 expression) in 34.7% (74/213) of RMS samples (15/60 alveolar, 54/143 embryonal, 5 RMS not otherwise specified), but negative or negligible in all other sarcoma samples.ConclusionsThis is the first study of GPC3 in a large series of sarcomas, and highlights expression of GPC3 in RMS but not other STS. As glypicans are rarely expressed in mature tissue, GPC3 may be a therapeutic target in RMS, and as the protein can be shed from the cell surface, it may have future application as a biomarker for this disease.
The safety and efficacy of coiling multiple aneurysms in the same session
Authors: Xavier Andrew R , Rayes Mahmoud , Pandey Paritosh , Tiwari Ambooj , Kansara Amit , Guthikonda Murali 
Published: April 2011

ObjectiveMultiple intracranial aneurysms are common. While sequential clipping of multiple aneurysms during a single open surgical procedure has been reported, the same is not true for endovascular coiling. We present our experience describing the safe coiling of multiple aneurysms in the same setting.MethodsRetrospective review of our coiling log between 2006 and 2009 showed six cases in which multiple aneurysms were coiled in the same session.ResultsAll patients were coiled using the same microcatheter. Distal aneurysms were coiled first. Good occlusion rates were achieved in all cases. There were no thromboembolic events or procedure-related rupture or mortality.ConclusionIn addition to safety and efficacy, cost savings are expected when coiling multiple aneurysms in the same procedure.
Trigeminal neuralgia: the diagnosis and management of this excruciating and poorly understood facial pain
Authors: Zakrzewska Joanna M , McMillan Roddy 
Published: April 2011

ABSTRACT
Trigeminal neuralgia is defined as sudden, usually unilateral, severe, brief, stabbing recurrent episodes of pain within the distribution of one or more branches of the trigeminal nerve, which has a profound effect on quality of life. The diagnosis is made on history alone, and time needs to be taken to elicit the key features and differentiate from toothache or one of the trigeminal autonomic cephalalgias. Most trigeminal neuralgia is idiopathic, but a small percentage is due to secondary causes—for example, tumours or multiple sclerosis—which can be picked up on CT or MRI. Recently published international guidelines suggest that carbamazepine and oxcarbazepine are the first-line drugs. There is limited evidence for the use of lamotrigine and baclofen. If there is a decrease in efficacy or tolerability of medication, surgery needs to be considered. A neurosurgical opinion should be sought early. There are several ablative, destructive procedures that can be carried out either at the level of the Gasserian ganglion or in the posterior fossa. The only non-destructive procedure is microvascular decompression (MVD). The ablative procedures give a 50% chance of patients being pain free for 4 years, compared with 70% of patients at 10 years after MVD. Ablative procedures result in sensory loss, and MVD carries a 0.2–0.4% risk of mortality with a 2–4% chance of ipsilateral hearing loss. Surgical procedures result in markedly improved quality of life. Patient support groups provide information and support to those in pain and play a crucial role.
Effects of fentanyl on isoflurane minimum alveolar concentration and cardiovascular function in mechanically ventilated goats
Authors: Dzikiti T. B. , Stegmann G. F. , Dzikiti L. N. , Hellebrekers L. J. 
Published: April 2011

The effects of fentanyl on the minimum alveolar concentration (MAC) of isoflurane and cardiovascular function in mechanically ventilated goats were evaluated using six healthy goats (three does and three wethers). Following induction of general anaesthesia with isoflurane delivered via a mask, endotracheal intubation was performed and anaesthesia was maintained with isoflurane. The baseline MAC of isoflurane (that is, the lowest alveolar concentration required to prevent gross purposeful movement) in response to clamping a claw with a vulsellum forceps was determined. Immediately after baseline isoflurane MAC determination, the goats received, on separate occasions, one of three fentanyl treatments, administered intravenously: a bolus of 0.005 mg/kg followed by constant rate infusion (CRI) of 0.005 mg/kg/hour (treatment LFENT), a bolus of 0.015 mg/kg followed by CRI of 0.015 mg/kg/hour (treatment MFENT) or a bolus of 0.03 mg/kg followed by CRI of 0.03 mg/kg/hour (treatment HFENT). Isoflurane MAC was redetermined during the fentanyl CRI treatments. Cardiopulmonary parameters were monitored. A four‐week washout period was allowed between treatments. The observed baseline isoflurane MAC was 1.32 (1.29 to 1.36) per cent. Isoflurane MAC decreased to 0.98 (0.92 to 1.01) per cent, 0.75 (0.69 to 0.79) per cent and 0.58 (0.51 to 0.65) per cent following LFENT, MFENT and HFENT respectively. Cardiovascular function was not adversely affected. The quality of recovery from general anaesthesia was good, although exaggerated tail‐wagging was observed in some goats following MFENT and HFENT.
The Transcription Factor Evi-1 Is Overexpressed, Promotes Proliferation, and Is Prognostically Unfavorable in Infratentorial Ependymomas
Authors: Koos Björn , Bender Sebastian , Witt Hendrik , Mertsch Sonja , Felsberg Jörg , Beschorner Rudi , Korshunov Andrey , Riesmeier Barbara , Pfister Stefan , Paulus Werner , Hasselblatt Martin 
Published: April 2011

Abstract
Purpose: Ependymomas are glial tumors of presumably radial glial origin that share morphologic similarities with ependymal cells. The molecular genetics of ependymomas of supratentorial, infratentorial, and spinal location is heterogeneous. We aimed at identifying pathways operative in the development of infratentorial ependymomas.
Experimental Design: To do so, gene expression profiles of tumor cells laser microdissected from infratentorial ependymomas (n = 15) were compared with that of nonneoplastic ependymal cells laser microdissected from autopsy tissue (n = 7).
Results: Among 31 genes significantly overexpressed (&gt;5-fold) in ependymomas, transcription factor EVI1 (ecotropic viral integration site 1) showed the highest overexpression (35-fold). Evi-1 protein expression could be confirmed in formalin-fixed, paraffin-embedded samples of 26 of 28 infratentorial ependymomas but only in 7 of 47 nonependymal glial tumors (P &lt; 0.001). Furthermore, MDS1/EVI1 fusion transcripts were detectable in 17 of 28 infratentorial ependymomas and significantly correlated with MGMT (O6-methylguanine-DNA-methyltransferase) promoter hypermethylation (P &lt; 0.05). In primary infratentorial ependymoma cells, transfection with EVI1-specific siRNAs resulted in significant growth inhibition [48 hours: 87% ± 2% and 74% ± 10% as compared with control (mean ± SD; P &lt; 0.001)]. The prognostic role of EVI1 could further be validated in an independent cohort of 39 infratentorial and 26 supratentorial ependymomas on the basis of mRNA expression profiling. Although in supratentorial ependymomas EVI1 expression status had no prognostic impact, in infratentorial ependymomas, high EVI1 expression was associated with shorter overall survival and progression-free survival.
Conclusions: To conclude, the transcription factor Evi-1 is overexpressed in infratentorial ependymomas, promotes proliferation of ependymal tumor cells, and is prognostically unfavorable. Clin Cancer Res; 17(11); 3631–7. ©2011 AACR.
Relaxin Enhances the Collagenolytic Activity and In Vitro Invasiveness by Upregulating Matrix Metalloproteinases in Human Thyroid Carcinoma Cells
Authors: Bialek Joanna , Kunanuvat Usakorn , Hombach-Klonisch Sabine , Spens Antje , Stetefeld Joerg , Sunley Kevin , Lippert Dustin , Wilkins John A. , Hoang-Vu Cuong , Klonisch Thomas 
Published: April 2011

Abstract
In this study, we identified differential expression of immunoreactive matrix metalloproteinase 2 (MMP2)/gelatinase A, membrane-anchored MT1-MMP/MMP14, and human relaxin-2 (RLN2) in human benign and malignant thyroid tissues. MMP2 and MT1-MMP were detected in the majority of thyroid cancer tissues and colocalized with RLN2-positive cells. MMP2 was mostly absent in goiter tissues and, similar to RLN2, may serve as a marker for thyroid cancer. MMP2 and MT1-MMP were identified as novel RLN2 targets. RLN2 caused a significant downregulation of tissue inhibitor of MMP (TIMP) 3 protein levels but did not change the expression levels of MMP13, and TIMP1, TIMP2, and TIMP4 in human thyroid carcinoma cells. RLN2 failed to affect the expression of MMP1, 3, 8, and 9 in the thyroid carcinoma cells investigated. Stable RLN2 transfectants secreted enhanced levels of bioactive MMP2 which contributed to the increased collagenolytic activity and in vitro invasiveness into collagen matrix by human thyroid cancer cells. Three-dimensional reconstitution of confocal fluorescent microscopy images revealed larger-sized invadopodia, with intense MT1-MMP accumulation at the leading migrating edge in RLN2 transfectants when compared with enhanced green fluorescent protein clones. In RLN2 transfectants actin stress fibers contributed to pseudopodia formation. In conclusion, enhanced tumor cell invasion by RLN2 involves the formation of MT1-MMP–enriched invadopodia that lead to increased collagenolytic cell invasion by human thyroid cancer cells. Mol Cancer Res; 9(6); 673–87. ©2011 AACR.
Celecoxib Inhibits Interleukin-6/Interleukin-6 Receptor–Induced JAK2/STAT3 Phosphorylation in Human Hepatocellular Carcinoma Cells
Authors: Liu Yan , Liu Aiguo , Li Huameng , Li Chenglong , Lin Jiayuh 
Published: April 2011

Abstract
Growing evidence shows an association between chronic liver inflammation and hepatocellular carcinoma (HCC) development. STAT3, which is associated with inflammation and cellular transformation, is constitutively activated in human HCC tissues but not in normal human liver tissues. Although interleukin-6 (IL-6) is elevated in the serum of patients with HCC, it is not fully understood whether STAT3 constitutive activation is positively correlated with autocrine IL-6 secreted by HCC cells. Here, we reported that in HCC cells, the elevated levels of both IL-6 and IL-6 receptor (IL-6R, gp80), not IL-6 alone, correlated with STAT3 activation. We also explored whether the anticancer effects of celecoxib, an anti-inflammatory drug, may be due to the inhibition of the IL-6/STAT3 pathway in HCC cells. Our results showed that celecoxib decreased STAT3 phosphorylation by reducing Janus-activated kinase (JAK2) phosphorylation and caused apoptosis in HCC cells. Celecoxib could also block exogenous IL-6–induced STAT3 phosphorylation and nuclear translocation. Moreover, we observed more significant inhibition of cell viability when celecoxib was combined with doxorubicin or sorafenib. We conclude that the elevated levels of IL-6/IL-6R may be correlated with STAT3 activation in HCC cells. Celecoxib may be a candidate for HCC therapy through blocking IL-6/STAT3 pathway and can be combined with other anticancer drugs to reduce drug resistance caused by IL-6/STAT3 signals. Cancer Prev Res; 4(8); 1296–305. ©2011 AACR.
Role of Complement Cascade in Abdominal Aortic Aneurysms
Authors: Hinterseher Irene , Erdman Robert , Donoso Larry A. , Vrabec Tamara R. , Schworer Charles M. , Lillvis John H. , Boddy Amy M. , Derr Kimberly , Golden Alicia , Bowen William D. , Gatalica Zoran , Tapinos Nikos , Elmore James R. , Franklin David P. , Gray John L. , Garvin Robert P. , Gerhard Glenn S. , Carey David J. , Tromp Gerard , Kuivaniemi Helena 
Published: April 2011

Objective—
The goal of this study was to investigate the role of complement cascade genes in the pathobiology of human abdominal aortic aneurysms (AAAs).

Methods and Results—

Results of a genome-wide microarray expression profiling revealed 3274 differentially expressed genes between aneurysmal and control aortic tissue. Interestingly, 13 genes in the complement cascade were significantly differentially expressed between AAA and the controls.
In silico
analysis of the promoters of the 13 complement cascade genes showed enrichment for transcription factor binding sites for signal transducer and activator of transcription (STAT)5A. Chromatin-immunoprecipitation experiments demonstrated binding of transcription factor STAT5A to the promoters of the majority of the complement cascade genes. Immunohistochemical analysis showed strong staining for C2 in AAA tissues.

Conclusion—
These results provide strong evidence that the complement cascade plays a role in human AAA. Based on our microarray studies, the pathway is activated in AAA, particularly via the lectin and classical pathways. The overrepresented binding sites of transcription factor STAT5A in the complement cascade gene promoters suggest a role for STAT5A in the coordinated regulation of complement cascade gene expression.
Coffee Consumption and Incidence of Heart Failure in Women
Authors: Levitan Emily B. , Ahmed Hanna N. , Mittleman Murray A. , Wolk Alicja 
Published: April 2011

Background—
Previous studies of the relationship between coffee consumption and incidence of heart failure (HF) have not been consistent, with both potential benefit and potential harm reported. We therefore examined the association between coffee consumption and HF hospitalization or mortality in women.

Methods and Results—

We conducted a prospective, observational study of 34 551 participants of the Swedish Mammography Cohort who were 48 to 83 years old and did not have HF, diabetes, or myocardial infarction at baseline. Diet was measured using food-frequency questionnaires. Cox models were used to calculate hazard ratios of HF hospitalization or death from HF as the primary cause, as determined through the Swedish inpatient and cause-of-death registers between January 1, 1998, and December 31, 2006. Over 9 years of follow-up, 602 HF events occurred. Women who consumed ≥5 cups of coffee per day did not have higher rates of HF events than those who consumed <5 cups per day (multivariable-adjusted hazard ratio, 0.93; 95% confidence interval, 0.72 to 1.20). Compared with women who consumed ≤1 cup of coffee per day, hazard ratios were 1.01, 0.82, 0.94, and 0.87 for women who consumed 2, 3, 4, and ≥5 cups per day, respectively (
P
for trend=0.23). Further adjustment for self-reported hypertension did not change the results.

Conclusions—
In this population of middle-aged and older women, we did not find an association between coffee consumption and incidence of HF events.