bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sustainable innovation policy in non-walled technoparks
Authors: Sarikaya Ozkeser Banu 
Published: May 2024

Meanwhile, the government in different countries encourages these sustainable activities to make their domestic brands open worldwide Because sustainability and learning growth have comparable meanings, all paths leading in this direction cross with a single phenomenon known as innovation. The more investment in innovation, the more value the company can gain. In Turkey, the government has increased the financial support of sustainable innovation management for more than 20 years. Especially, in the last five years the budget of the ministry has widened to keep growing in growth. The grant incentives given for Research &Development (R&D) centers and Design centers, also called non-walled technoparks, are two main examples of this encouragement. This study is framed on a big-scale company’s growth with the help of the R&D or Design Center. The financial and performance-based milestones in the R&D center road map lead the company to follow and invest in technology. This also means taking advantage of the international competition in 360 degrees. Not only is financial performance increased by gaining incentives, but also the qualification of human resources is going up and up. The results show how sustainable innovation policy influences business activities.

Keywords: Brand; Design center; R&D center; sustainable ınnovation.
Evaluating the Influence of the Forestry Reclamation Approach on Throughfall Quantity in Eastern Kentucky
Authors: Gerlitz Morgan , Agouridis Carmen , Williamson Tanja , Barton Christopher 
Published: May 2024

Reclamation Highlights:
Rapid growth and canopy closure by conifers resulted in higher canopy interception rates than those observed in deciduous trees, both on reforested mine sites and in natural forests. Results from this study show the importance of species survival rates on interception and that low survival rates could negatively impact, or at least delay, the hydrologic recovery of a reclaimed area and potentially threaten landscape stability. Use of the forestry reclamation approach can help restore hydrologic function on reclaimed surface mines.
Tuberculous Flexor Tenosynovitis with Median Nerve Compression
Authors: Khandekar Pushkar , Patil Sandeep , Korday Sujit , Gopikrishna Madhusudan , Kulkarni Shradha , Jain Kevin 
Published: May 2024

Case:
A 65-year-old female patient presented with complaints of diffuse pain and swelling in her right wrist with paresthesia in her right hand with thenar wasting. Her magnetic resonance imaging scan was suggestive of flexor tenosynovitis of the wrist with compression of the median nerve with multiple rice bodies. She underwent excisional biopsy along with median nerve decompression. Mycobacterium tuberculosis was detected by polymerase chain reaction (GeneXpert), and histopathology identified caseous granulomas. The patient was started on antitubercular chemotherapy postoperatively.

Conclusion:
In endemic countries such as India, tuberculous flexor tenosynovitis must always be a differential diagnosis in cases of wrist swelling with rice bodies.
CapTrap-Seq cDNA library preparation for full-length RNA sequencing
Authors: Carbonell-Sala Sílvia , Guigó Roderic 
Published: May 2024

Abstract

Long-read RNA sequencing is crucial for generating precise and comprehensive annotations of eukaryotic genomes. Despite advancements in throughput and accuracy, achieving reliable end-to-end identification of RNA transcripts remains a challenge for long-read sequencing methods. To address this limitation, we developed CapTrap-seq, a cDNA library preparation method that combines the Cap-trapping strategy with oligo(dT) priming to detect 5' capped, full-length transcripts.
CapTrap-seq is a competitive open-source, platform-agnostic RNA library preparation method for producing full-length transcript sequences.
Single-cell profiling of small molecule-genomic interactions and epigenetic states
Authors: Dong Chao , Meng Xiaoxuan , Zhang Tong , Guo Zhifang , Liu Yaxi , Wu Peihuang , Chen Shiwei , Zhou Fanqi , Ma Yanni , Shu Shaokun , He Aibin 
Published: May 2024

Abstract

We present scEpiChem, a unique method to jointly measure the genomic interactions of small molecules and epigenetic states, such as histone modifications and chromatin accessibility, within single cells. scEpiChem utilizes split-pool barcoding strategy and protein A-Tn5 (PAT)-anti-biotin antibody to capture biotinylated small molecule binding sites within individual cells. Through sequential targeted tagmentation by different PAT-antibody complexes or Tn5, scEpiChem allows for the simultaneous detection of small molecule drug-target engagement and multimodal epigenome. The efficacy of the protocol is demonstrated through the profiling of biotinylated inhibitors JQ1, THZ1, and Dox. This approach provides valuable insights into cell state-specific drug targeting and gene regulation, facilitating the development of precise therapeutic interventions and the exploitation of drug resistance mechanisms.
In vitro compatibility of herbicides with bionematicides and action on Meloidogyne javanica
Authors: Ribeiro Paula Fernanda de Azevedo , Machado Andressa Cristina Zamboni , Matsumoto Leopoldo Sussumu , Lameu Nathalia Dutra , Canteri Marcelo Giovanetti 
Published: May 2024

Abstract

This study aimed to assess the compatibility of herbicides with Bacillus firmus, Purpureocillium lilacinum, Pochonia chlamydosporia, and Trichoderma harzianum, as well as their impact on Meloidogyne javanica. The experiments were conducted under a completely randomized design with nine treatments and five replicates. The treatments included control, diclosulam, sulfentrazone, flumioxazine, imazethapyr, S-metolachlor, pyroxasulfone, imazethapyr + flumioxazine, and glyphosate. For B. firmus, colony forming units per milliliter (CFU mL− 1) were counted; for fungi, the mycelial growth speed index (IVCM – mm day− 1) and percentage of inhibition were measured; and for nematodes, the mortality rate was determined. Results showed that imazethapyr, S-metolachlor, and glyphosate were incompatible with B. firmus. Regarding P. lilacinum, P. chlamydosporia, and T. harzianum, these herbicides, along with sulfentrazone, exhibited the highest inhibition percentages. The most significant mortality rates of M. javanica were observed with imazethapyr, flumioxazine, and S-metolachlor. In conclusion, S-metolachlor, imazetapyr, glyphosate, and sulfentrazone were identified as the least compatible with the tested biological nematicides.
Supplementation effects of bamboo leaves on intake, growth performance, and carcass yield of lambs fed grasses as basal diets indoors versus under field conditions in Sidama Highland, Ethiopia
Authors: Debeko Denbeshu , Tolera Adugna , Angassa Ayana 
Published: May 2024

Abstract

We evaluate the effects of bamboo leaf supplementation on feed intake, body weight gain, and carcass yield of Arsi-Bale lambs. Eight treatments were used with different levels of inclusions at 150 g (T2), 300 g (T3), and 450 g (T4) in both groups (i.e., indoor-fed vs. grazed in the field). T1 and T5 were control treatments for indoor-fed and those grazed in the field, respectively. A 2 x 4 factorial experiment was used with T1-T4 for indoor feeding and T5 -T8 for those grazed in the field. Carcass evaluation was carried out for all lambs. Feed analysis and evaluation of the nutritional quality of feed samples were examined. The results showed a significant (P < 0.000) increase in the nutrient and dry matter (DM) intake and growth performance of lambs with increased levels of bamboo leaves supplementation. There was a significant (P < 0.000) increase in the average daily gain of indoor-fed lambs from 33.33 to 62.67 g/day. The feed conversion efficiency of indoor-fed lambs was within the range of 0.057 to 0.084. The results confirmed that fresh bamboo leaf supplementation had a positive effect on the growth performance and carcass yields of lambs with improved DM intake. We suggest that the inclusion of fresh bamboo leaves is crucial to improve the performance of lambs. Increased inclusion of bamboo leaf supplementation in the lambs’ diet resulted in improved body weight gain and would be a viable option to boost sheep production.
Integrated Approaches for Rapid Screening of Salt-tolerant Chrysanthemum Genotypes
Authors: Jadhav Pritam R. , Jagtap Apurva Y. , Shinde Nalini A. , Sarode Dipak K. , Pagariya Madhuri C. , Prasad KV , Kawar Prashant Govindrao 
Published: May 2024

Abstract

Ten Chrysanthemum morifolium genotypes were assessed for their responses to different salt concentrations at biochemical, molecular and anatomical dimensions. Biochemical analysis revealed variations in enzyme activities and lipid peroxidation levels, shedding light on salt stress response mechanisms of chrysanthemum. Notably, different degrees of tolerance were observed among the genotypes, with Bidhan Lalima and Bidhan Sabita showing high salt tolerance, while Bidhan Jayanti and Silk Brocate as highly salt susceptible. Molecular analysis indicated higher expression of stress-responsive gene and transcription factors in stress-susceptible genotypes, suggesting predictable variations in stress response mechanisms. Specifically, CmSOD, CmWRKY10, CmNCED3A, CmHSFA4, and CmHSP70 genes played distinct roles in salt mitigation mechanisms in chrysanthemum genotypes. Root anatomical studies revealed genotype-specific root anatomy, indicating distinct root structures in tolerant and susceptible genotypes to cope with salt stress. This study underscores the combined utility of biochemical, molecular, and anatomical approaches for rapid screening of salt-tolerant chrysanthemum genotypes. The score plot distinguished the genotypes under study as highly tolerant, moderately tolerant and susceptible genotypes based on their response to salt stress. Correlation analysis depicted a strong correlation of MDA with chitinase and POD activity in chrysanthemum genotypes under salt stress. These findings provide valuable insights for future breeding programs aimed at developing salt-tolerant chrysanthemum genotypes for sustainable floriculture practices.
Flow Tweezing of Anisotropic Magnetic Microrobots in a Dynamic Magnetic Trap for Active Retention and Localized Flow Sensing
Authors: Liu Yuan , Cao Quanliang , Xu Haifeng , Lin Gungun 
Published: May 2024

Abstract

Controlled manipulation of microscale robotic devices in complex fluidic networks is critical for various applications in biomedical endovascular sensing, lab-on-chip biochemical assays, and environmental monitoring. However, achieving controlled transport and active retention of microscale robots with flow sensing capability have proven challenging. Here, we report the dynamic tweezing of an anisotropic magnetic microrobot in a rotating magnetic trap for active retention and localized flow sensing under confined fluidic conditions. We reveal a series of unconventional motion modes and dynamics of the microrobot transporting in a confined fluidic flow, which manifest themselves as transitions from on-trap centre rolling to large-area revolution and off-trap centre rolling with varying rotating frequencies. By retaining the robot within the magnetic trap and its motion modulated by the field frequency, the off-centre rolling of the microrobot endows a crucial localized flow sensing capabilities, including flow rate and flow direction determination. The magnetic microrobot serves as a mobile platform for measuring the flow profile along a curved channel, mimicking a blood vessel. Our findings unlock a new strategy to determine local magnetic tweezing force profile and flow conditions in arbitrary flow channels, revealing strong potential for microfluidics, chemical reactors, and in-vivo endovascular flow measurement.
Co and N2 Are No Humane Alternatives to Co2 Euthanasia in Rats.
Authors: Seebeck Petra , Calvet Charlotte , Villiger Philipp , Prisco Francesco , Buchholz Tim , Gantenbein Felix , Thöne-Reineke Christa , Gourévitch Boris , Wagner Carsten 
Published: May 2024

Abstract

Carbon dioxide (CO2) is commonly used for euthanasia of laboratory animals but has potential negative side effects. There is an urgent need to identify alternatives causing minimal distress while providing a fast and reliable loss of consciousness (LOC). We evaluated different CO2 concentrations (30% and 70%) and explored carbon monoxide (CO) and nitrogen (N2) as alternatives for a more humane euthanasia in rats. Isoflurane anesthesia was included as a reference. We assessed relevant physiological parameters in rats using a multifaceted approach combining EEG, ECG, telemetry, EMG, respiration, behavior, biochemical stress parameters, and lung histopathology. 70% CO2 resulted in rapid LOC and a faster death compared to 30% CO2 thus minimizing the time of consciousness in which distress and eventually pain can be experienced. Nevertheless, a higher amount of distress (e.g. gasping, arrhythmia or seizures) experienced until LOC might outweigh the benefits of a faster LOC. CO and N2 resulted in longer times to LOC, induced seizures before LOC, increased stress levels and caused higher lung damage. Therefore, CO and N2 are no humane alternatives to CO2 and should not be used for euthanasia of rodents. However, combining CO2 with anesthetic gases might be suitable to induce a more humane euthanasia.
USP4 promotes proliferation and metastasis in human lung adenocarcinoma
Authors: WEI YAMIN , WEI SHANWANG , LEI ZHONGTENG , WU JINXIAO , HUANG JINLI , FU LIJUAN , HUANG GUIYING , LI ZHIMENG , LIANG YUANNA , ZHENG JINHUA , ZHANG YAN 
Published: May 2024

Abstract

Objective
Research the expression of USP4 in lung adenocarcinoma and its correlation with clinicopathological features and prognosis analysis, to explore the invasion and metastasis mechanism of USP4 in lung adenocarcinoma, and to clarify the mechanism of USP4's involvement in the occurrence and development of lung adenocarcinoma.
Methods
The expressions of USP4, VEGF, MMP2 and Ki67 in lung adenocarcinoma and adjacent tissues of 139 patients with lung adenocarcinoma were detected by immunohistochemical method, and the correlation between expression and clinicopathological features and survival curve were analyzed by statistical method. The expression of USP4 was interfered by LIP-2000 cell transfection technology, and the expression of USP4 and its related factors in protein level was detected by Western Blot, and their correlation was analyzed. After silencing USP4 expression, the effects of USP4 on proliferation, invasion and migration of lung adenocarcinoma cells were detected by cell scratches assay, MTT assay, Transwell assay and tumorigenesis assay in nude mice.
Results
The expression of USP4 in lung adenocarcinoma tissues was higher than that in normal adjacent tissues, and the high expression of USP4 was significantly correlated with the differentiation degree of lung adenocarcinoma, clinical stage and pathological grade lymph node metastasis. After silencing USP4 expression, the expression of cyclin apoptosis protein invasion related proteins and phosphorylation factors were affected, and then cell migration after silencing USP4 expression The proliferation ability decreased, the number of invasion and metastasis decreased, and the tumor volume decreased in nude mice.
Conclusions
USP4 may play a certain role in the invasion and metastasis of lung adenocarcinoma by regulating the expression of tumor-related factors and affecting the prognosis of patients with lung adenocarcinoma. USP4 can be used as a potential therapeutic target for clinical diagnosis of lung adenocarcinoma and provide a new opportunity for clinical research on lung adenocarcinoma.
Uncovering Potential Genetic Targets in the Mediating Role of Demyelinating Disease for Multiple Sclerosis-Induced Neuropathic Bladder: A Mendelian Randomization Analysis
Authors: Xu Yuangao , Wu Yikun , Xiong Jieyu , Wu Xiaoyu , Shi Hua , Xu Shuxiong 
Published: May 2024

Abstract

Objective Despite the lack of a genetic explanation for the causal link between multiple sclerosis (MS) and neuropathic bladder (NPB), our study aims to explore this causality and underlying mechanisms using Mendelian Randomization (MR), aiming to identify novel protein targets for future therapeutic interventions.Methods Data pertaining to MS, demyelinating diseases (DD), neurogenic bladder, and plasma proteins were sourced from the IEU Open GWAS Project and encompassed a diverse population. After stringent screening, a bidirectional two-sample MR analysis was first conducted to establish the causality between MS and NPB. DD was then introduced as a mediator for further testing via the product of coefficients approach. Subsequently, plasma proteins were analyzed as exposures against the aforementioned phenotypes to screen for potential therapeutic targets.Results Our analysis substantiates that MS is associated with an increased risk of developing NPB (P-value = 0.001), with no evidence of reverse causality, reinforcing the unidirectional impact of MS on NPB. The mediation analysis revealed a clear causal pathway, supporting the hypothesis that DD serves as a crucial intermediary in the progression from MS to NPB (P-value = 0.005, mediation proportion = 70.29%). Notable proteins such as ADAM11, GRIA4, CXCL13, and PRKCG were identified, and by relaxing the FDR constraints, GSR and UBA2 were also pinpointed as potential risk factors for both MS and NPB.Conclusions Our MR analysis elucidated the causal connections among MS, DD, and NPB from a genetic perspective, identifying potential protein targets that facilitate future drug development and therapeutic strategies.
Phase sensitive information from a planar Josephson junction
Authors: Yuan Andrew , Kivelson Steven 
Published: May 2024

Abstract

We analyze both the general symmetry-related and more microscopic considerations that govern the Josephson tunnelling across a finite planar junction between a known $s$-wave superconductor and a candidate unconventional superconductor (e.g., $d_{x^2-y^2}$-wave). Due to the finite size of the probe, the Josephson current possesses an edge contribution, which is shown to be the dominant contribution under certain conditions. Thus, the dependence of the edge contribution on the geometry of the junction can serve as a direct probe of the symmetry of the order parameter in the unconventional superconductor.
Agricultural carbon emissions in Henan counties: Influence mechanism, regional differences and spatial effects
Authors: Cui Weiwei , Yin Minghao , Xie Yifan , Lu Yiheng , Huang Longjunjiang 
Published: May 2024

Abstract

Exploring the effective and efficient path of agricultural carbon emission reduction in Henan Province is of great significance to optimizing the strategic layout of China's agricultural emission reduction and carbon sequestration. Accordingly, we first scientifically measure the agricultural carbon emissions of each county and then utilize the standard deviation ellipse and spatial measurement model to scientifically analyze and clarify the spatial and temporal evolution trend and spatial effect mechanism of agricultural carbon emissions in Henan Province based on the county data of Henan Province from 2010 to 2020. The results show that: (1) In 2020, the total agricultural carbon emissions in Henan Province will be 134.7274 million tons, with the distribution of high in the southeast and low in the northwest, which has gradually become balanced, and the center of gravity is mainly concentrated in Xuchang county. (2) The spatial dependence of agricultural carbon emissions in Henan Province shows a four-stage trend of "fluctuating down ~ continuing up ~ plummeting ~ fluctuating up again", and the spatial heterogeneity is dominated by the "low-low" agglomeration, and the "high-low" agglomeration is dominated by the "low-low" agglomeration. The spatial heterogeneity is dominated by "low-low" agglomeration, followed by "high-low" agglomeration. (3) There is an "inverted U" curve relationship between the level of agricultural economic development and agricultural carbon emissions, the latter increases and then decreases as the former increases. The increase in the level of agricultural mechanization and urbanization rate will significantly reduce agricultural carbon emissions. The opposite is true for the financial support for agriculture, the income level of rural residents, and the structure of the agricultural industry. (4) In terms of spatial spillover effects, the increase in the level of agricultural development in neighboring counties will first increase and then decrease agricultural carbon emissions in this county. The mechanization level and urbanization rate of neighboring counties will reduce agricultural carbon emissions in this county, and vice versa for the income level of rural residents and the scale utilization of agricultural land.
Microfluidic Ecology Unravels the Genetic and
Ecological Drivers of T4r Bacteriophage Resistance
in E. coli: Insights into Biofilm-Mediated Evolution
Authors: Austin Robert , Nagy Krisztina , Valappil Sarshad , Phan Trung , Li Shengkai , Dér László , Morris Ryan , Bos Julia , Winslow Sophia , Galajda Peter , Rákhely Gábor 
Published: May 2024

Abstract

We use a microfluidic ecology which generates non-uniform phage concentration gradients and micro-ecological niches to reveal the importance of time, spatial population structure and collective population dynamics in the {\em de novo} evolution of T4r bacteriophage resistant motile {\em E. coli}. An insensitive bacterial population against T4r phage occurs within 20 hours in small interconnected population niches created by a gradient of phage virions, driven by evolution in transient biofilm patches. Sequencing of the resistant bacteria reveals mutations at the receptor site of bacteriophage T4r as expected but also in genes associated with biofilm formation and surface adhesion, supporting the hypothesis that evolution within transient biofilms drives {\em de novo} phage resistance.