bluuebunny/crossref_metadata_2025_split huggingface dataset:
Relationship between human IgG structure and retention time in hydroxyapatite chromatography with sodium‐phosphate gradient elution
Authors: Nakagawa Taishiro , Ishihara Takashi , Yoshida Hideaki , Yoneya Takashi , Wakamatsu Kaori , Kadoya Toshihiko 
Published: November 2009

AbstractThe aim of this study was to classify the retention time of recombinant human monoclonal antibodies (rhMAbs) in hydroxyapatite chromatography with sodium‐phosphate gradient elution according to their physicochemical properties. We analyzed 37 rhMAbs and found that (i) the structures of both the constant and variable regions affected retention time, (ii) the number of basic amino acid residues in the variable region, particularly in the heavy chain, correlated well with retention time, and (iii) this correlation was more pronounced (e.g. r2=0.87 for 18 κ IgG1 rhMAbs) when the surface accessibility of those residues are taken into account. These findings provide a useful guide for investigators and purification‐process developers working with monoclonal antibodies.
Comparison of the influence of organic modifier on the secondary interactions of polar embedded and classical alkyl‐silica reversed phase HPLC stationary phases
Authors: McGachy Neil T. , Zhou Lili 
Published: November 2009

AbstractLinear solvation energy relationships are used along with solute retention data to compare the influence of organic modifier on the secondary interactions of polar‐embedded (EPG) and classical alkyl‐silica (C18) stationary phases. System constants derived using the linear solvation energy relationship model measure the relative strength of solute interactions in the mobile phase and the solvated stationary phase. Differences in the e and s system constants, which, respectively, measure interactions of π and lone pair electrons and dipole‐type interactions, were larger between the two stationary phases when methanol was used versus ACN. A similar effect was not evident for the a system constant, which is a measure of hydrogen‐bond acceptor ability. The b system constant, which measures hydrogen‐bond donating ability, was consistently lower for the EPG stationary phases when water–methanol mobile phases were used, especially at lower methanol levels. Plots of the polar and specific selectivity for select solutes versus organic modifier level are used to demonstrate the differing influences of ACN and methanol on the selectivity obtained on representative EPG and C18 stationary phases. For the solutes studied, differences in both the polar and specific selectivity were increased between the two stationary phases when water–methanol mobile phases were used.
Microwave‐assisted extraction for the simultaneous determination of Novolac glycidyl ethers, bisphenol A diglycidyl ether, and its derivatives in canned food using HPLC with fluorescence detection
Authors: Zhang Hong , Xue Ming , Lu Yanbin , Dai Zhiyuan , Wang Honghai 
Published: November 2009

AbstractA microwave‐assisted extraction (MAE) protocol and an efficient HPLC analysis method were first developed for the fast extraction and simultaneous determination of bisphenol F diglycidyl ether (Novolac glycidyl ether 2‐Ring), Novolac glycidyl ether 3‐Ring, Novolac glycidyl ether 4‐Ring, Novolac glycidyl ether 5‐Ring, Novolac glycidyl ether 6‐Ring, bisphenol A diglycidyl ether, bisphenol A (2,3‐dihydroxypropyl) glycidyl ether, bisphenol A (3‐chloro‐2‐hydroxypropyl) glycidyl ether, bisphenol A bis(3‐chloro‐2‐hydroxypropyl) ether, bisphenol A (3‐chloro‐2‐hydroxypropyl) (2,3‐dihydroxypropyl) ether in canned fish and meat. After being optimized in terms of solvents, microwave power and irradiation time, MAE was selected to carry out the extraction of ten target compounds. Analytes were purified by poly(styrene‐co‐divinylbenzene) SPE columns and determinated by HPLC‐fluorescence detection. LOD varied from 0.79 to 3.77 ng/g for different target compounds based on S/N=3; LOQ were from 2.75 to 10.92 ng/g; the RSD for repeatability were <8.64%. The analytical recoveries ranged from 70.46 to 103.44%. This proposed method was successfully applied to 16 canned fish and meat, and the results acquired were in good accordance with the studies reported. Compared with the conventional liquid–liquid extraction and ultrasonic extraction, the optimized MAE approach gained the higher extraction efficiency (20–50% improved).
Intralesional bleomycin injections in the treatment of benign lymphoepithelial cysts of the parotid gland in HIV‐positive patients: Case reports
Authors: Monama Glen M. , Tshifularo Mashudu I. 
Published: November 2009

AbstractBenign lymphoepithelial cysts (BLCs) of the parotid gland are associated with human immunodeficiency virus. These cysts may grow large and uncomfortable, causing significant cosmetic embarrassment for the patient. Several treatment options have been described with varying successes. We report successful treatment of three adults with bleomycin. All three patients presented with bilateral parotid enlargements. They received a total dose of between 180 U and 270 U of bleomycin over a period of 6 to 10 weeks. They all responded completely without morbidity or recurrence. Bleomycin is effective in the treatment of BLCs of the parotid glands in HIV‐positive patients. Laryngoscope, 2010
Electromyographic laryngeal synkinesis alters prognosis in vocal fold paralysis
Authors: Statham Melissa M. , Rosen Clark A. , Smith Libby J. , Munin Michael C. 
Published: November 2009

AbstractObjectives/Hypothesis:Synkinesis, or misdirected reinnervation, is likely a confounder when predicting return of function of an immobile vocal fold. Currently, no information exists on the incidence of synkinesis in unilateral vocal fold immobility (UVFI) or the effect synkinesis has on prognosis and treatment. Our objective was to examine a vocal fold adductor synkinesis screening protocol using diagnostic laryngeal electromyography (LEMG). We aim to determine the effect of synkinesis on prognosis of recovery of purposeful vocal fold motion.Study Design:Retrospective review of LEMG data and patient charts from laryngology practice.Methods:A standardized LEMG analysis method to diagnose vocal fold adductory synkinesis was performed in 124 consecutive laryngeal electromyographic exams.Results:Synkinesis testing was positive in 12/124 patients (9.7%). Post hoc quantitative analysis of electromyographic recordings to compare motor unit potential amplitude in the thyroarytenoid/lateral cricoarytenoid complex during sustained phonation to those in the same muscle during a “sniff” revealed a significant difference in motor unit potential amplitude ratio for control subjects (0.32), those who recovered purposeful vocal fold motion (0.40), and those with vocal fold paralysis (0.96) (P = .001). The presence of synkinesis in patients with UVFI improved the negative predictive value of LEMG from 53% to 100% and the sensitivity from 56% to 100%.Conclusions:Presence of laryngeal synkinesis using motor amplitude ratio criteria, in the setting of good voluntary motor unit recruitment and UVFI, downgrades a patient's prognosis to one that is poor for recovery. We propose this screening protocol as an adjunct to diagnostic LEMG. Laryngoscope, 2010
Laryngeal neuropathy of Charcot‐Marie‐Tooth disease: Further observations and novel mutations associated with vocal fold paresis
Authors: Benson Brian , Sulica Lucian , Guss Joel , Blitzer Andrew 
Published: November 2009

AbstractObjectives/Hypothesis:To describe and define laryngeal neuropathy in Charcot‐Marie‐Tooth (CMT) disease.Study Design/Methods:Retrospective record review from a university laryngology practice.Results:Four adult CMT patients presented with laryngeal symptoms. Three patients exhibited bilateral vocal fold palsy, in each case with more severe hypomobility on the left. One case exhibited an isolated left vocal fold palsy. All patients complained of hoarseness and stridor, three had dyspnea, two patients had dysphagia, and one had obstructive sleep apnea (OSA). One patient has required airway surgery to date. Genetic testing revealed known sequence alterations in one case and sequence alterations previously not associated with laryngeal dysfunction in two cases. One case was familial and two were sporadic; information is not available in a fourth.Conclusions:The clinical course of the cases suggests slowly progressive neuropathy that appears to be nerve length dependent. The lack of severe respiratory distress despite dense bilateral paresis is consistent with existing reports and with the reported low rate of tracheostomy in adults with laryngeal manifestations of CMT. Genetic testing does not currently inform expectations or management of laryngeal disease. Dyspnea, dysphagia, and OSA symptoms in patients with CMT require careful laryngologic evaluation. Laryngoscope, 2010
A FGF3 mutation associated with differential inner ear malformation, microtia, and microdontia
Authors: Ramsebner Reinhard , Ludwig Martin , Parzefall Thomas , Lucas Trevor , Baumgartner Wolf‐Dieter , Bodamer Olaf , Cengiz Filiz Basak , Schoefer Christian , Tekin Mustafa , Frei Klemens 
Published: November 2009

AbstractObjectives/Hypothesis:Analysis of association between genotype and phenotype.Study Design:Prospective genetic study in a family.Methods:Auditory investigations, computer tomography, and genetic sequencing of the fibroblast growth factor 3 (FGF3) gene were performed on a Somali family presenting with autosomal recessive, hearing impairment, microdontia, and outer ear morphologies ranging from normal auricle development to microtia assessed as type 1 Weerda dysplasia in affected individuals.Results:Computed tomography imaging identified differential inter‐ and intraindividual malformations of the inner ear including labyrinth aplasia, development of a common cavity to the presence of a cochlear with 1.5 windings (Mondini malformation) in affected individuals, symptoms similar to those described as labyrinth aplasia, microtia, and microdontia (LAMM) syndrome, caused by mutations in FGF3. Genetic sequencing revealed the presence of a novel p.R95W missense mutation in FGF3 segregating with pathology. The p.R95W mutation substitutes a positively charged arginine for a polar tryptophan in the highly conserved RYLAM consensus of the β6 sheet of FGF3 that interacts with FGFR2.Conclusions:These findings describe, for the first time, variable inner ear malformations and outer ear dysplasia in the presence of constant microdontia, associated with homozygous inheritance of the p.R95W mutation in FGF3, mirroring phenotypes observed in mouse models ablating FGF3/FGFR2 signaling. Laryngoscope, 2010
Laryngopharyngeal sensory deficits and impaired pharyngeal motor function predict aspiration in patients irradiated for nasopharyngeal carcinoma
Authors: Ku Peter K. M. , Vlantis Alexander C. , Leung Sing Fai , Lee Kathy Y. S. , Cheung Dilys M. C. , Abdullah Victor J. , van Hasselt Andrew , Tong Michael C. F. 
Published: November 2009

AbstractObjectives/Hypothesis:To assess the contribution of laryngopharyngeal sensory deficits and impaired pharyngeal motor function to aspiration in patients irradiated for nasopharyngeal carcinoma.Study Design:A retrospective study at a tertiary referral university teaching hospital.Methods:One hundred consecutive patients who underwent radiotherapy for nasopharyngeal carcinoma referred to a dysphagia clinic underwent sensory testing of their laryngopharynx and endoscopic evaluation of their swallowing. The sensory threshold of the laryngopharynx was determined, the pharyngeal contraction assessed, and the status of the larynx and hypopharynx documented before and after swallowing. The presence of laryngeal penetration and aspiration was noted.Results:The average time from radiation therapy to assessment was 10.2 years, and the mean duration of swallowing symptoms was 27 months. Laryngopharyngeal sensation was deficient in 89% of patients and the pharyngeal contraction impaired in 93% patients. Laryngeal penetration and aspiration occurred in 87% and 74% of patients, respectively. Aspiration was associated with food residue in the pyriform fossae after swallowing (P < .001) and impaired pharyngeal contraction (P < .001), but not with laryngopharyngeal sensory deficiency. There was no association between a laryngopharyngeal sensory deficit and impaired pharyngeal contraction.Conclusions.Impaired pharyngeal contraction and food bolus clearance from the hypopharynx during swallowing are more important than laryngopharyngeal sensory deficiency in predicting aspiration in patients who underwent radiotherapy for nasopharyngeal carcinoma. Laryngoscope, 2010
Parathyroidectomy in the elderly population: Does age really matter?
Authors: Young VyVy N. , Osborne Kyra M. , Fleming Muffin M. , Flynn Michael B. , Goldstein Richard E. , Bumpous Jeffrey M. 
Published: November 2009

AbstractObjectives/Hypothesis:Compare parathyroidectomy patients based on age, including demographics, outcomes, and complications.Study Design:Retrospective review.Methods:Prospective parathyroidectomy database covering 1998 to 2007 was reviewed retrospectively.Results:A total of 687 patients underwent parathyroidectomy, including 247 (36%) >65 years old. Discharge was more often on day of surgery in younger patients (42.5% vs. 29.2%, P = .007) and >23 hours for older patients (24.7% vs. 12.3%, P < .0001). Older patients stayed longer in the recovery room (134 vs. 107 minutes, P = .005). Despite postoperative normocalcemia, older patients tended to have persistently elevated parathyroid hormone (PTH) (10.5% vs. 6.4%, P = .07), whereas younger patients had normal PTH (81.6% vs. 70%, P = .0007). PTH levels were low‐abnormal (56–110) in younger patients (47% vs. 29%, P = .046), but high‐abnormal (>220) in older patients (16.6% vs. 9.55%, P = .009). Overall complication rates were low (6%–8%), with >93% in either group having no major complications. There was no difference in timing or types of complications, except elderly patients were more likely to have cardiac complications (2.83% vs. 0.45%, P = .022).Conclusions:Nearly 700 parathyroidectomies were performed at our institution over 10 years. Elderly patients comprised one third of this population. They were likely to have longer hospital and recovery room stays, and postoperative normocalcemia with elevated PTH, which may actually be a normal finding for these patients, but it warrants further study. The rate, timing, and types of complications were similar between age groups, although elderly patients had more cardiac complications. When properly indicated, parathyroidectomy remains a safe and effective option for management of hyperparathyroidism in elderly patients. Laryngoscope, 2010
Sustained delivery of lidocaine into the cochlea using poly lactic/glycolic acid microparticles
Authors: Horie Rie T. , Sakamoto Tatsunori , Nakagawa Takayuki , Tabata Yasuhiko , Okamura Noboru , Tomiyama Naoki , Tachibana Mitsuhiro , Ito Juichi 
Published: November 2009

AbstractObjectives/Hypothesis:Lidocaine is a local anesthetic that is known to suppress tinnitus via systemic or local application; however, this effect has only limited duration. The current study aimed to establish a method for the sustained delivery of lidocaine into the cochlea using poly lactic/glycolic acid (PLGA) microparticles.Study Design:Experimental study.Methods:Lidocaine‐loaded PLGA microparticles were produced and their in vitro‐release profiles were examined. The lidocaine concentrations in the perilymph were measured at different time points following the application of the lidocaine‐loaded PLGA microparticles to the round‐window membranes of guinea pigs. The possible adverse effects of the local application of lidocaine‐loaded PLGA microparticles were also examined.Results:The in vitro analyses revealed that the microparticles were capable of the sustained delivery of lidocaine. The in vivo experiments demonstrated the sustained delivery of lidocaine into the cochlear fluid, and the maintenance of high lidocaine concentrations in the perilymph for up to 3 days after application. Nystagmus and inflammation in the middle ear mucosa were not detected after the local application of lidocaine‐loaded PLGA microparticles, although temporary hearing loss was observed.Conclusions:Lidocaine‐loaded PLGA microparticles were shown to be capable of the sustained delivery of lidocaine into the cochlea, suggesting that they could be used for the attenuation of peripheral tinnitus. Laryngoscope, 2010
7Li NMR chemical shift titration and theoretical DFT calculation studies: solvent and anion effects on second‐order complexation of 12‐crown‐4 and 1‐aza‐12‐crown‐4 with Lithium cation in several aprotic solvents
Authors: Masiker Marilyn C. , Mayne Charles L. , Boone Brian J. , Orendt Anita M. , Eyring Edward M. 
Published: November 2009

Abstract7Li NMR titration was used to determine stepwise complexation constants for the second‐order complexation of lithium cation with 12‐crown‐4 in acetonitrile, propylene carbonate and a binary mixture of propylene carbonate and dimethyl carbonate. The anions used were perchlorate, hexaflurophosphate and trifluromethanesulfonate. A second ligand 1‐aza‐12‐crown‐4 was similarly investigated. The exchange between the free and complexed cation in these reactions is fast on an NMR timescale resulting in a single lithium peak which is a concentration‐weighted average of the free and bound species. Solvent effects show that the 1:1 complex is much more stable in acetonitrile than in propylene carbonate or in the propylene carbonate dimethyl carbonate mixture. Anion effects for a given solvent were small. Optimized geometries of the free ligands and the 1:1 and 1:2 (sandwich) metal–ligand complexes were predicted by hybrid‐density functional theory using the Gaussian 03 software package. Results were compared to literature values for 1:1 stability constants found by microcalorimetry for several of these systems and are found to be in good agreement. Although microcalorimetry only considered the formation of 1:1 complexes, 7Li NMR shows evidence that both 1:1 and 1:2 complexes should be considered. Copyright © 2009 John Wiley & Sons, Ltd.
The Mandel–Cryer effect in chemoporoelasticity
Authors: Bunger A. P. 
Published: November 2009

AbstractChemoporoelastic theory is an extension of classical Biot poroelasticity that accounts for coupling with the presence and the transport of ions in the pore fluid. The impact of this extra level of coupling can be both substantial and complex. This paper relies on the two variations of Mandel's classical problem, which has become a canonical illustration of the complexity that poromechanical coupling can bring to an otherwise straightforward system. To this end, solutions for a chemoporoelastic shale cylinder and a spherical shale ball are derived. These solutions are then used to demonstrate that chemoporoelastic coupling leads to a coupled pore pressure response that is not only non‐monotonic, as in Mandel's classical case, but also demonstrates the consequences of the semi‐permeable membrane‐like nature of the shale and of the problem's two diffusion‐related timescales. This paper concludes with a discussion of the implications of these results for experimentation and modeling of so‐called reactive shales using chemoporoelastic theory. Copyright © 2009 John Wiley & Sons, Ltd.
Detection of necrotic neural response in super‐acute cerebral ischemia using activity‐induced manganese‐enhanced (AIM) MRI
Authors: Inoue Yasuo , Aoki Ichio , Mori Yuki , Kawai Yuko , Ebisu Toshihiko , Osaka Yasuhiko , Houri Takashi , Mineura Katsuyoshi , Higuchi Toshihiro , Tanaka Chuzo 
Published: November 2009

AbstractImmediate and certain determination of the treatable area is important for choosing risky treatments such as thrombolysis for brain ischemia, especially in the super‐acute phase. Although it has been suggested that the mismatch between regions displaying ‘large abnormal perfusion’ and ‘small abnormal diffusion’ indicates a treatable area on an MRI, it has also been reported that the mismatch region is an imperfect approximation of the treatable region named the ‘penumbra’. Manganese accumulation reflecting calcium influx into cells was reported previously in a middle cerebral artery occlusion (MCAO) model using activity‐induced manganese‐enhanced (AIM) MRI. However, in the super‐acute phase, there have been no reports about mismatches between areas showing changes to the apparent diffusion coefficient (ADC) and regions that are enhanced in AIM MRI. It is expected that the AIM signal can be enhanced immediately after cerebral ischemia in the necrotic core region due to calcium influx. In this study, a remote embolic rat model, created using titanium‐oxide macrospheres, was used to observe necrotic neural responses in the super‐acute phase after ischemia. In addition, images were evaluated by comparison between ADC, AIM MRI, and histology. The signal enhancement in AIM MRI was detected at 2 min after the cerebral infarction using a remote embolic method. The enhanced area on the AIM MRI was significantly smaller than that on the ADC map. The tissue degeneration highlighted by histological analysis corresponded more closely to the enhanced area on the AIM MRI than that on the ADC map. Thus, the manganese‐enhanced region in brain ischemia might indicate ‘necrotic’ irreversible tissue that underwent calcium influx. Copyright © 2009 John Wiley & Sons, Ltd.
Credit scores, race, and residential sorting
Authors: Nelson Ashlyn Aiko 
Published: November 2009

AbstractCredit scores have a profound impact on home purchasing power and mortgage pricing, yet little is known about how credit scores influence households' residential location decisions. This study estimates the effects of credit scores on residential sorting behavior using a novel mortgage industry data set combining household demographic, credit, and financial data with property location information and detailed community attribute data. I employ the data set to estimate a discrete‐choice residential sorting model. I find that credit scores significantly predict residential sorting behavior and models that do not account for credit score provide biased estimates of housing utilities for black households in particular. Simulation results show that increases in credit score are associated with increases in the consumption of higher‐priced homes in more expensive school districts, higher‐quality public schools, and proximity to urban/metropolitan areas. © 2010 by the Association for Public Policy Analysis and Management.
Elderly immigrants' labor supply response to supplemental security income
Authors: Kaushal Neeraj 
Published: November 2009

AbstractThis paper examined how the 1996 Personal Responsibility and Work Opportunity Reconciliation Act, which banned Supplemental Security Income (SSI) for the majority of elderly immigrants, affected their employment, retirement, and family incomes. The policy was found to be associated with a 3.5 percentage point (9.5 percent) increase in the employment and a 3.8 percentage point (7 percent) decrease in the retirement of foreign‐born elderly men. Partly as a result of their employment response, SSI ineligibility and the consequent decline in SSI receipt did not have any statistically significant effects on the family incomes of elderly foreign‐born men. Noncitizen elderly women, on the other hand, did not experience any increase in employment, and those without family support suffered a 10 to 17 percent decline in income. These findings suggest that access to SSI did not create work disincentives for noncitizen elderly women and that SSI restrictions have imposed financial hardship on those without any family support, many of whom perhaps cannot effectively increase their employment. © 2010 by the Association for Public Policy Analysis and Management.