bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immediate dental rehabilitation in fibula free flaps for malignancy: Is it feasible?
Authors: Jelmini Jonathan , Williams Fayette C. , Winstead Michael , Hammer Daniel A. , Kim Roderick Y. 
Published: May 2024

AbstractBackgroundFibula free flap reconstruction has revolutionized maxillofacial reconstruction. While immediate dental rehabilitation with dental implants and teeth has shown benefits, it remains uncommon, especially for patients with malignancy.MethodsA retrospective cohort study at a single institution explored immediate dental rehabilitation in fibula flaps for patients with malignant disease. Thirteen patients with malignancies that underwent immediate fibula free flap reconstruction with dental implants and dental prosthesis were included with a minimum of 3‐month follow‐up.ResultsForty eight implants replaced 90 teeth in 13 patients. All implants were integrated at 3 months, with an overall success rate of 87.5%. Two patients experienced delayed (>3 months postoperatively) implant loss due to osteoradionecrosis and infection. Peri‐mucositis occurred in three patients which resolved with treatment. Skin paddles were used in 11 patients and radiation therapy was not delayed for any patient.ConclusionWith proper patient selection, diagnosis of malignancy and the need for osteocutaneous flap reconstruction does not exclude the ability to place immediate implants and deliver an immediate dental prosthesis in head and neck reconstruction.
A Highly Efficient Click Linker for Enrichment of Alkyne‐Tagged Proteins in Living Cells
Authors: Qin Guoting , Duong Shara , Fu Yunxin , Cai Chengzhi 
Published: May 2024

AbstractAlkyne tags have been widely used for the enrichment of labeled proteins to enable their profiling at a proteome‐wide scale by mass spectrometry. The key component in the enrichment process is an azido‐terminated cleavable linker for capturing the labeled proteins/peptides via click reaction. Herein, we report a new efficient click linker (APA biotin) featuring an acid‐cleavable acetal linkage end‐caped with a highly reactive picolyl azido head and a biotin handle for anchoring onto streptavidin‐coated supports. Using an amine‐reactive probe to profile the proteome structural changes in living S. cerevisiae cells within 5 minutes of heat shock, we demonstrated that the linker allowed identification of >9400 labeled sites, among which 457–1656 with significantly altered reactivity upon heat shock. This study represented the first chemical labeling mass spectrometry (CL–MS)‐based profiling of proteome structural changes in living cells in response to external stimuli. Data are available via ProteomeXchange with identifier PXD051279.
Single‐element amorphous metals
Authors: Han Xiao , Wu Geng , He Dong Sheng , Hong Xun 
Published: May 2024

AbstractTo unveil the nature of amorphous states, single‐element amorphous metals have been the perfect research subject due to the simplest composition. However, the extreme crystal nucleation and growth rate in single‐element metal make the synthesis of single‐element amorphous metals seemingly impossible in the past. Fortunately, benefited by several delicate synthetic strategies developed recently, the single‐element amorphous metals have been successfully demonstrated. This review aims to provide a systematic overview of the synthesis of single‐element amorphous metals covering the challenges in physics and recent achievements. In addition, current understanding of the atomic and electronic structures of single‐element amorphous metal has also been included. Finally, the challenges that worth further investigation are discussed. By identifying the potential avenues for further exploration, this review aims to contribute valuable insights that will propel the cognition of single‐element amorphous metals.
A dual‐range Janus‐structure pressure sensor with broad detection range and high resolution combining triboelectricity and piezoelectricity
Authors: Zhao Shilong , Chen Chaojie , Wang Zhiyuan , Pan Caofeng , Yang Cheng 
Published: May 2024

AbstractEnabling pressure sensors with high resolution and a broad detection range is of paramount importance yet challenging due to the limitations of each known sensing method. Overlying different sensing mechanisms to achieve complementary functions is a promising approach, but it often leads to increased device thickness, crosstalk signals and complex signal channel management. Herein, we present a dual‐functional conformable pressure sensor that adopts a Janus thin film layout, enabling simultaneous piezoelectric and triboelectric signal detection capabilities between just one electrode pair, showing a most compact device configuration. Notably, despite its thin thickness (~80 μm for a packaged device), it exhibits a broad‐range detection capability with high signal resolution and fast response time, demonstrating a distinct signal‐relay characteristic corresponding to piezoelectricity and triboelectricity. Despite the slimness and simple structure, it shows an impressive signal resolution of 0.93 V·kPa−1 in the range of 0.1–140 kPa and 0.05 V·kPa−1 in the range of 140–380 kPa. Moreover, the device fabrication can be combined with the kirigami method to improve fitting to joint surfaces. This work introduces an innovative paradigm for designing advanced pressure sensing mechanisms, enabling a single device that can meet diverse application scenarios through its simplicity, slim layout, conformable, and self‐powered characteristics to adapt to multiple scenarios.image
Propagation process‐based agricultural drought typology and its copula‐based risk
Authors: Li Liang , Liu Jiangzhou , Peng Qing , Wang Xiaowen , Xu Jiatun , Cai Huanjie 
Published: May 2024

AbstractDetermining the risks associated with different drought event types can be beneficial for related scientific research and management strategies. In this study, we proposed an agricultural drought event pair and typology based on the governing drought propagation processes in the Yellow River basin at the catchment scale. In total, seven agricultural drought event pairs were distinguished, namely single meteorological drought event pair (MDonly), single soil moisture drought event pair (SDonly), single agricultural drought event pair (ADonly), soil moisture and meteorological drought event pair (SDMD), agricultural and meteorological drought event pair (ADMD), agricultural and soil moisture drought event pair (ADSD) and agricultural, soil moisture and meteorological drought event pair (ASMD). The SDMD and ADMD events had the highest copula‐based risk probabilities with the shortest joint return periods. Six agricultural drought types were distinguished in this study, namely classical rainfall deficit, rain‐to‐snow‐season, wet‐to‐dry‐season, cold snow season, warm snow season and composite drought events. The classical rainfall deficit, wet‐to‐dry season and composite drought events were the major agricultural drought types in the Yellow River basin. The agricultural drought typology results of the present study provide a comprehensive understanding of drought propagation and improvement of drought forecasting and management.
Impact of protein conformations on binding free energy calculations in the beta‐secretase 1 system
Authors: Baumann Hannah M. , Mobley David L. 
Published: May 2024

AbstractIn binding free energy calculations, simulations must sample all relevant conformations of the system in order to obtain unbiased results. For instance, different ligands can bind to different metastable states of a protein, and if these protein conformational changes are not sampled in relative binding free energy calculations, the contribution of these states to binding is not accounted for and thus calculated binding free energies are inaccurate. In this work, we investigate the impact of different beta‐sectretase 1 (BACE1) protein conformations obtained from x‐ray crystallography on the binding of BACE1 inhibitors. We highlight how these conformational changes are not adequately sampled in typical molecular dynamics simulations. Furthermore, we show that insufficient sampling of relevant conformations induces substantial error in relative binding free energy calculations, as judged by a variation in calculated relative binding free energies up to 2 kcal/mol depending on the starting protein conformation. These results emphasize the importance of protein conformational sampling and pose this BACE1 system as a challenge case for further method development in the area of enhanced protein conformational sampling, either in combination with binding calculations or as an endpoint correction.
Integrated multi‐omics approach reveals the role of striated muscle preferentially expressed protein kinase in skeletal muscle including its relationship with myospryn complex
Authors: Li Qifei , Lin Jasmine , Luo Shiyu , Schmitz‐Abe Klaus , Agrawal Rohan , Meng Melissa , Moghadaszadeh Behzad , Beggs Alan H. , Liu Xiaoli , Perrella Mark A. , Agrawal Pankaj B. 
Published: May 2024

AbstractBackgroundAutosomal‐recessive mutations in SPEG (striated muscle preferentially expressed protein kinase) have been linked to centronuclear myopathy with or without dilated cardiomyopathy (CNM5). Loss of SPEG is associated with defective triad formation, abnormal excitation–contraction coupling, calcium mishandling and disruption of the focal adhesion complex in skeletal muscles. To elucidate the underlying molecular pathways, we have utilized multi‐omics tools and analysis to obtain a comprehensive view of the complex biological processes and molecular functions.MethodsSkeletal muscles from 2‐month‐old SPEG‐deficient (Speg‐CKO) and wild‐type (WT) mice were used for RNA sequencing (n = 4 per genotype) to profile transcriptomics and mass spectrometry (n = 4 for WT; n = 3 for Speg‐CKO mice) to profile proteomics and phosphoproteomics. In addition, interactomics was performed using the SPEG antibody on pooled muscle lysates (quadriceps, gastrocnemius and triceps) from WT and Speg‐CKO mice. Based on the multi‐omics results, we performed quantitative real‐time PCR, co‐immunoprecipitation and immunoblot to verify the findings.ResultsWe identified that SPEG interacts with myospryn complex proteins CMYA5, FSD2 and RyR1, which are critical for triad formation, and that SPEG deficiency results in myospryn complex abnormalities (protein levels decreased to 22 ± 3% for CMYA5 [P < 0.05] and 18 ± 3% for FSD2 [P < 0.01]). Furthermore, SPEG phosphorylates RyR1 at S2902 (phosphorylation level decreased to 55 ± 15% at S2902 in Speg‐CKO mice; P < 0.05), and its loss affects JPH2 phosphorylation at multiple sites (increased phosphorylation at T161 [1.90 ± 0.24‐fold], S162 [1.61 ± 0.37‐fold] and S165 [1.66 ± 0.13‐fold]; decreased phosphorylation at S228 and S231 [39 ± 6%], S234 [50 ± 12%], S593 [48 ± 3%] and S613 [66 ± 10%]; P < 0.05 for S162 and P < 0.01 for other sites). On analysing the transcriptome, the most dysregulated pathways affected by SPEG deficiency included extracellular matrix–receptor interaction (P < 1e−15) and peroxisome proliferator‐activated receptor signalling (P < 9e−14).ConclusionsWe have elucidated the critical role of SPEG in the triad as it works closely with myospryn complex proteins (CMYA5, FSD2 and RyR1), it regulates phosphorylation levels of various residues in JPH2 and S2902 in RyR1, and its deficiency is associated with dysregulation of several pathways. The study identifies unique SPEG‐interacting proteins and their phosphorylation functions and emphasizes the importance of using a multi‐omics approach to comprehensively evaluate the molecular function of proteins involved in various genetic disorders.
Modeling of a linear ion trap with driving rectangular waveforms
Authors: Ivanov A. I. , Sysoev A. A. , Konenkov A. N. , Konenkov N. V. 
Published: May 2024

AbstractWe consider the operation of a digital linear ion trap with resonant radial ejection. A sequence of rectangular voltage pulses with a dipole resonance signal is applied to the trap electrodes. The periodic waveform is piecewise constant, has zero mean, and is determined by an asymmetry parameter : one value is taken on interval and another on , where is the RF period. Ion mass scanning is performed by varying the asymmetry parameter and amplitude of the negative pulse part with time. The ion oscillation frequencies and acceptance of the linear trap are calculated. The dependence of the ion mass to charge ratio on the parameter is . The maximum value is about kDa for typical parameters of the linear trap: frequency 0.5 MHz, rod radius 4 mm, and negative pulse amplitude 1 kV. The dipolar excitation frequency is 0.125 MHz at which the LIT acceptance is maximal.
Dual coherent anti‐Stokes Raman scattering microscopy across the vibrational spectrum enabled by single pump optically synchronised oscillators and spectral focusing
Authors: Gudavičius Dominykas , Kontenis Lukas , Langbein Wolfgang 
Published: May 2024

AbstractCoherent anti‐Stokes Raman scattering microscopy probing two independently selectable vibrational frequencies across the vibrational spectrum is demonstrated. A one‐box femtosecond laser source provides three synchronised 100–200 fs pulses of 77 MHz repetition rate, one at a fixed wavelength of 1025 nm from an Yb oscillator and two independently tuneable ones, over 680–960 nm and 960–1300 nm, respectively, from two optically synchronised parametric oscillators. Combined with spectral focusing, the source allows addressing the fingerprint, cell silent and C–H stretch vibrational regions simultaneously, avoiding the motion artefacts seen in sequential imaging. We demonstrate the microscopy method on water/heavy water/oil emulsions, plastic beads and deuterated lipids inside cells.
Combining endpoint and change data did not affect the summary standardised mean difference in pairwise and network meta‐analyses: An empirical study in depression
Authors: Ostinelli Edoardo G. , Efthimiou Orestis , Luo Yan , Miguel Clara , Karyotaki Eirini , Cuijpers Pim , Furukawa Toshi A. , Salanti Georgia , Cipriani Andrea 
Published: May 2024

AbstractWhen studies use different scales to measure continuous outcomes, standardised mean differences (SMD) are required to meta‐analyse the data. However, outcomes are often reported as endpoint or change from baseline scores. Combining corresponding SMDs can be problematic and available guidance advises against this practice. We aimed to examine the impact of combining the two types of SMD in meta‐analyses of depression severity. We used individual participant data on pharmacological interventions (89 studies, 27,409 participants) and internet‐delivered cognitive behavioural therapy (iCBT; 61 studies, 13,687 participants) for depression to compare endpoint and change from baseline SMDs at the study level. Next, we performed pairwise (PWMA) and network meta‐analyses (NMA) using endpoint SMDs, change from baseline SMDs, or a mixture of the two. Study‐specific SMDs calculated from endpoint and change from baseline data were largely similar, although for iCBT interventions 25% of the studies at 3 months were associated with important differences between study‐specific SMDs (median 0.01, IQR −0.10, 0.13) especially in smaller trials with baseline imbalances. However, when pooled, the differences between endpoint and change SMDs were negligible. Pooling only the more favourable of the two SMDs did not materially affect meta‐analyses, resulting in differences of pooled SMDs up to 0.05 and 0.13 in the pharmacological and iCBT datasets, respectively. Our findings have implications for meta‐analyses in depression, where we showed that the choice between endpoint and change scores for estimating SMDs had immaterial impact on summary meta‐analytic estimates. Future studies should replicate and extend our analyses to fields other than depression.
Retention mechanisms of amitriptyline and its impurities on amide, amino, diol, and silica columns in hydrophilic interaction liquid chromatography
Authors: Knežević Ratković Darija , Kukobat Radovan , Kasagić‐Vujanović Irena 
Published: May 2024

Hydrophilic interaction liquid chromatography (HILIC) has been widely applied to challenging analysis in biomedical and pharmaceutical fields, bridging the gap between normal‐phase high‐performance liquid chromatography and reversed‐phase high‐performance liquid chromatography (RP‐HPLC). This paper comprehensively explores the retention mechanisms of amitriptyline and its impurities A, B, C, D, F, and G on amide, amino, diol, and silica columns. Dual HILIC/RP‐HPLC retention mechanisms were developed, and transitional points between HILIC and RP‐HPLC mechanisms were calculated on amide, diol, and silica columns. Adsorption and partition contributions to overall retention mechanisms were evaluated using Python software in HILIC and RP‐HPLC regions. The cation exchange mechanism dominates overall retention for ionized analytes in the silica column (R2 > 0.995), whereas the retention of ionized analytes increases with pH. Impacts of acetonitrile content, buffer ionic strength, and pH, along with their interactions on the retention of ionized analytes in the silica column, were determined using the chemometric approach. Acetonitrile content showed the most significant impact on the retention mechanisms. These findings highlight that a detailed investigation into retention mechanisms provides notable insights into factors influencing analyte retention and separation, promising valuable guidance for future analysis.
Cutaneous manifestations of anti‐synthetase syndrome: Case series and literature review
Authors: Chan Airiss R. , Osman Mohamed , Yacyshyn Elaine , Gniadecki Robert 
Published: May 2024

AbstractBackgroundAnti‐synthetase syndrome (ASyS) is a rare inflammatory myopathy associated with anti‐aminoacyl transfer RNA synthetase antibodies, and is characterized by a triad of interstitial lung disease, arthritis, and myopathy. ASyS has been considered to represent a subtype of dermatomyositis (DM), however it is not clear whether cutaneous findings overlap in both diseases.ObjectivesWe aimed to characterize mucocutaneous features which should raise suspicion of ASyS and prompt early diagnostic testing.MethodsWe reviewed 28 cases of ASyS in our institution, and retrieved data from 43 articles reporting clinical features of ASyS in the literature.ResultsDermatologic manifestations of ASyS can be categorized as: specific cutaneous signs strongly associated with ASyS (‘Mechanic's hands’, ‘Hiker's feet’); mucocutaneous signs overlapping with other collagenoses; nailfold changes (resembling those of DM and systemic sclerosis); and mucocutaneous signs of undetermined significance. Only 20% had pathognomonic DM signs.ConclusionsDermatologic manifestations of ASyS are heterogeneous and do not always reproduce the pattern seen in DM. Presence of palmar hyperkeratosis (‘Mechanic's hands’), cuticular overgrowth, skin ulcerations and nailfold capillary changes should prompt further diagnostic workup of ASyS. Limitations of our study include inconsistent reporting of dermatologic findings within our series between virtual and in‐person visits. Not all patients were seen in dermatology consultation.
The impact of ethical leadership on KM practices and performance
Authors: Durst Susanne , Foli Samuel , Temel Serdal 
Published: May 2024

AbstractRecent advancements in leadership research emphasise the influential role of ethical leadership in shaping positive employee behaviour. However, there remains unclear the precise impact of ethical leadership on organisational performance and its interaction with knowledge creation and knowledge networks. Drawing on both the knowledge‐based view and the ethical leadership literature, this study asserts a positive influence of ethical leadership on both organisational performance and knowledge creation. Furthermore, we propose that knowledge creation positively impacts knowledge networks, which, in turn, exert a positive influence on organisational performance. To validate these propositions, we employ structural equation modelling on a dataset comprising 205 Turkish firms. Our study contributes to the (ethical) leadership literature as one of the initial attempts to examine the relationship between ethical leadership and organisational performance while considering the roles of knowledge creation and knowledge networks.
Microbial Composition of the Laryngotracheal Region: A Systematic Review
Authors: Sragi Zara , Vasan Vikram , Laitman Benjamin M. , Rivera‐Rivera Gabriel , Powers Ann , Dickstein Daniel R. , Kirke Diana N. , Courey Mark S. 
Published: May 2024

IntroductionMicrobiome research has predominantly focused on the oral cavity and oropharynx's role in disease, while the upper airway, specifically the larynx and trachea, has been relatively overlooked. Examining the microbial communities in these regions can shed light on how dysbiosis influences diseases and their management. This review evaluates laryngotracheal microbial compositions in both healthy and diseased patients.MethodsWe conducted a systematic review in EMBASE, MEDLINE, and Cochrane Central databases, yielding 1383 studies in the initial search. Inclusion criteria involved participants aged over 18 years and the use of next‐generation 16s ribosomal sequencing methods.ResultsWe included 10 studies—seven focused on larynx sequencing and four on trachea sequencing (one investigated both sites). In a healthy larynx, diverse species such as Streptococcus, Cloacibacterium, Prevotella, and Helicobacter were found. Benign laryngeal diseases exhibited reduced microbial diversity, mainly dominated by Streptococcus. Subglottic stenosis patients showed diminished diversity in both idiopathic and iatrogenic scars. Laryngeal squamous cell carcinoma displayed increased diversity, primarily featuring Fusobacterium. Among non‐respiratory‐compromised surgery patients, the tracheal microbiome was more diverse in diabetics and those later developing lower respiratory infections. Pneumonia patients exhibited an abundance of Prevotella and Streptococcus, linked to an increased 28‐day survival rate, while Streptococcus and Haemophilus abundance correlated with successful extubation.ConclusionsThe laryngotracheal region hosts a unique microbial community influenced by both benign and malignant conditions. Many lesions remain unexplored, underscoring the need for future studies encompassing diverse laryngotracheal conditions. Clinical trials assessing microbiome modifications may unveil novel therapeutic avenues.Level of EvidenceNA Laryngoscope, 134:4167–4175, 2024