bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Kaposi's sarcoma‐associated herpesvirus G protein‐coupled receptor: Lessons on dysregulated angiogenesis from a viral oncogene
Authors: Jham Bruno C. , Montaner Silvia 
Published: March 2010

AbstractTumor viruses can induce cell transformation by overcoming cellular defense mechanisms and promoting the ungoverned proliferation of infected cells. To this end, functionally related viral oncogenes have evolved in disparate viruses to over‐ride key proliferative and survival intracellular pathways, thus assuring efficient viral replication and contributing to tumor formation. Indeed, the study of viral oncogenes has been a powerful tool for disclosing fundamental insights into these basic cellular processes. In this regard, the Kaposi's sarcoma‐associated herpesvirus (KSHV or HHV8), the etiological agent of Kaposi's sarcoma (KS), is an exemplary model of an oncogenic virus that includes within its genome several homologues of cellular genes implicated in the regulation of cell proliferation and apoptosis. However, emerging evidence now points to a single KSHV gene, ORF74, encoding for the viral G protein‐coupled receptor (vGPCR), as essential for KS development. Expressed in only a fraction of cells within KS lesions, this viral receptor induces tumorigenesis through both autocrine and paracrine mechanisms. Indeed, work from several laboratories has demonstrated that vGPCR can promote cell proliferation, enhance cell survival, modulate cell migration, stimulate angiogenesis, and recruit inflammatory cells, both in expressing cells, as well as in neighboring (bystander) cells. Examination of this powerful viral oncogene may expose novel targets for the treatment of patients with KS and could ultimately provide a unique perspective into how GPCRs, and specifically chemokine receptors, contribute to angiogenesis and tumorigenesis. J. Cell. Biochem. 110: 1–9, 2010. © 2010 Wiley‐Liss, Inc.
ATM is involved in cell‐cycle control through the regulation of retinoblastoma protein phosphorylation
Authors: Pizarro Javier G. , Folch Jaume , de la Torre Aurelio Vazquez , Junyent Felix , Verdaguer Ester , Jordan Joaquin , Pallas Merce , Camins Antoni 
Published: March 2010

AbstractAtaxia telangiectasia mutated protein (ATM) is a member of the phosphatidylinositol‐3 kinase (PI3K) family, which has a role in the cellular response to DNA double‐strand breaks (DSBs). In the present study, we evaluated the role of ATM in cell‐cycle control in dopaminergic rat neuroblastoma B65 cells. For this purpose, ATM activity was either inhibited pharmacologically with the specific inhibitor KU‐55933, or the ATM gene was partially silenced by transfection with small interfering RNA (siRNA). Our data indicate that although ATM inhibition did not affect the cell cycle, both treatments specifically decreased the levels of cyclin A and retinoblastoma protein (pRb), phosphorylated at Ser780. Furthermore, ATM inhibition decreased the active form of p53, which is phosphorylated at Ser15, and also decreased Bax and p21 expression. Using H2O2 as a positive control of DSBs, caused a rapid pRb phosphorylation, this was prevented by KU‐55933 and siRNA treatment. Collectively, our data demonstrate how a new molecular network on ATM regulates the cell cycle through the control of pRb phosphorylation. These findings support a new target of ATM. J. Cell. Biochem. 110: 210–218, 2010. © 2010 Wiley‐Liss, Inc.
Survey of the year 2008: applications of isothermal titration calorimetry
Authors: Falconer Robert J. , Penkova Anita , Jelesarov Ilian , Collins Brett M. 
Published: March 2010

AbstractIsothermal titration calorimetry (ITC) is a fast, accurate and label‐free method for measuring the thermodynamics and binding affinities of molecular associations in solution. Because the method will measure any reaction that results in a heat change, it is applicable to many different fields of research from biomolecular science, to drug design and materials engineering, and can be used to measure binding events between essentially any type of biological or chemical ligand. ITC is the only method that can directly measure binding energetics including Gibbs free energy, enthalpy, entropy and heat capacity changes. Not only binding thermodynamics but also catalytic reactions, conformational rearrangements, changes in protonation and molecular dissociations can be readily quantified by performing only a small number of ITC experiments. In this review, we highlight some of the particularly interesting reports from 2008 employing ITC, with a particular focus on protein interactions with other proteins, nucleic acids, lipids and drugs. As is tradition in these reviews we have not attempted a comprehensive analysis of all 500 papers using ITC, but emphasize those reports that particularly captured our interest and that included more thorough discussions we consider exemplify the power of the technique and might serve to inspire other users. Copyright © 2010 John Wiley & Sons, Ltd.
Do all patients with carcinoma of the esophagus benefit from treatment at teaching facilities?
Authors: Cheung Michael C. , Koniaris Leonidas G. , Yang Relin , Zhuge Ying , MacKinnon Jill A. , Byrne Margaret M. , Franceschi Dido 
Published: March 2010

AbstractBackgroundWe sought to determine whether patients with esophageal carcinoma benefit from regionalization of care.MethodsThe Florida Cancer Data System (FCDS) and the Agency for Health Care Administration data sets (1998–2002) were merged and queried.ResultsA total of 5,041 patients (87.6% Caucasian vs. 11.1% African American (AA)) demonstrated a median survival time of 9.8 months overall and 23.4 months following surgical resection (P < 0.001). Adenocarcinoma arose predominantly in Caucasian patients (98.1%). Patients with adenocarcinoma (n = 2,248) derived a treatment benefit at a TF (HR = 1.35, P = 0.003), including an improved 90‐day mortality following surgery (2.1% vs. 4.0%, P < 0.001). Squamous cell carcinoma (SCC) arose predominantly in AA patients (91.6%). No overall survival benefit at TF was observed (HR = 1.01, P = 0.915), however a trend for reduced 90‐day surgical mortality was observed at TF (1.9% vs. 5.2%, P = 0.062). Multivariate analysis for adenocarcinoma demonstrates that poverty, lack of chemotherapy or surgery, and failure to provide treatment at a TF are independent predictors of worse survival. For SCC patients, AA race was a significant predictor of poorer survival while TF and poverty level were not.ConclusionsThese data suggest no benefit from potential regionalized care for patients with squamous histology, which disproportionately affects AA. J. Surg. Oncol. J. Surg. Oncol. 2010;102:18–26. © 2010 Wiley‐Liss, Inc.
Association of the C677T polymorphism in the methylenetetrahydrofolate reductase gene with sudden sensorineural hearing loss
Authors: Uchida Yasue , Sugiura Saiko , Ando Fujiko , Shimokata Hiroshi , Nakashima Tsutomu 
Published: March 2010

AbstractObjectives/Hypothesis:To investigate the recently reported association of the C677T polymorphism in the methylenetetrahydrofolate reductase (MTHFR) gene with sudden sensorineural hearing loss (SSNHL), we analyzed data from a community‐based Japanese population.Study Design:Nested case‐control study.Methods:Among 2,174 adults (1,096 males and 1,078 females) aged 40 to 79 years old who participated in the National Institute for Longevity Sciences–Longitudinal Study of Aging, we compared 33 cases of prevalent SSNHL, defined as a self‐reported otolaryngologist diagnosis, with the other cases. Multiple logistic regression was used to obtain odds ratios (ORs) for SSNHL in subjects with the MTHFR C677T polymorphism, with adjustment for other possibly influential factors under additive, dominant, and recessive genetic models.Results:The per‐allele ORs for SSNHL risk were 1.687 (95% confidence interval [CI], 1.023–2.780) in model 1, with adjustment for age and sex, and 1.654 (CI, 1.003–2.728) in model 2, with adjustment for smoking status, body mass index, histories of heart disease, hypertension, and diabetes, in addition to the factors in model 1. In model 3, a significant association between SSNHL and the C677T polymorphism was observed under all genetic models independent of factors including folic acid and homocysteine, although there were only 25 cases and 1,677 controls due to the addition of moderating factors.Conclusions:Our results suggest that the T allele of MTHFR C677T could be associated with susceptibility to SSNHL, and even imply that this mutation could be a risk factor that is independent of blood folic acid and homocysteine. Laryngoscope, 2010
Viscoelastic properties of phonosurgical biomaterials at phonatory frequencies
Authors: Kimura Miwako , Mau Ted , Chan Roger W. 
Published: March 2010

AbstractObjectives/Hypothesis:The purpose of this study was to examine the functional biomechanical properties of several injectable biomaterials currently or potentially used for vocal fold augmentation.Study Design:Rheometric investigation of phonosurgical materials in vitro.Methods:Linear viscoelastic shear properties of 3% bovine collagen (atelocollagen), micronized AlloDerm (Cymetra; LifeCell Corp., Branchburg, NJ), calcium hydroxylapatite (CaHA) (Radiesse; BioForm Medical, San Mateo, CA), and 2.4% cross‐linked hyaluronic acid (HA) gel (Juvéderm; Allergan, Inc., Irvine, CA) were quantified as functions of frequency covering the phonatory range, and compared to those of the human vocal fold cover. Measurements of elastic shear modulus (G′) and dynamic viscosity (η′) were made at up to 250 Hz with a controlled‐strain simple‐shear rheometer. Linear least‐squares regression was conducted to curve‐fit log G′ and log η′ versus log frequency, and statistical analysis was performed with one‐way analysis of variance.Results:Radiesse and Cymetra were found to be the stiffest and the most viscous materials, followed by Juvéderm and atelocollagen. There were significant differences in the magnitudes of G′ and η′ among the phonosurgical materials and the normal human vocal fold cover (p < .001), whereas there was no significant difference in the frequency dependence of G′ and η′ among the materials. Post hoc Tukey tests revealed significant differences (p < .05) in pairwise comparisons of the magnitudes of G′ and η′ among all materials and the vocal fold cover.Conclusions:These findings suggested that although these biomaterials may be injected lateral to the lamina propria for the treatment of glottic insufficiency, none of them are rheologically optimal for the functional reconstruction of the vocal fold lamina propria. Laryngoscope, 2010
Botulinum toxin injections for new onset bilateral vocal fold motion impairment in adults
Authors: Ekbom Dale C. , Garrett C. Gaelyn , Yung Katherine C. , Johnson Felicia L. , Billante Cheryl R. , Zealear David L. , Courey Mark S. 
Published: March 2010

AbstractObjectives/Hypothesis:Review of clinical experience and results using botulinum toxin type A (BTX) for the management of adult patients with respiratory compromise due to new onset bilateral vocal fold motion impairment (BVFMI).Study Design:Retrospective case series.Methods:The records of 11 patients from two institutions with respiratory compromise due to bilateral vocal fold motion impairment were reviewed. Age, sex, etiology of motion impairment, subjective response to BTX injections, changes in pulmonary function studies pre‐ and postinjection when available, the dosage of botulinum toxin required to achieve response, the number of injections per patient, and complications were reported.Results:All patients were over 18 years old. There were three male and eight female subjects. The etiology of BVFMI was due to previous anterior cervical surgery in nine patients and prolonged intubation in two. Ten patients reported symptomatic improvement and returned for an average of nine injections over the 10‐year period of study. The most common interval between injections was 3 months. In all patients the dose required to achieve symptomatic improvement was at least 2.5 mouse units injected into each vocal fold. One patient without relief of symptoms had bilateral cricoarytenoid joint fixation. Complications were limited to moderate dysphagia in one patient and breathy dysphonia in all patients.Conclusions:BTX injection into the vocal folds provides temporary relief of symptoms in airway obstruction in adult patients with BVFMI. Patients require an average of 2.5 units of botulinum injection into each vocal fold and have an average length of response of 3 months. BTX injection may be used as a form of temporary relief of airway obstruction in patients wishing to avoid ablative surgery or tracheotomy. Laryngoscope, 2010
Ambulatory pediatric otolaryngologic procedures in the United States: Characteristics and perioperative safety
Authors: Bhattacharyya Neil 
Published: March 2010

AbstractObjectives/Hypothesis:Determine the prevalence and safety of pediatric ambulatory otolaryngologic procedures in the United States.Study Design:Historical cohort study.Methods:All cases of pediatric otolaryngologic surgery were extracted from the National Survey of Ambulatory Surgery 2006 release. The population was characterized according to age, sex, and individual procedures performed. Rates of immediate perioperative complications were determined for arrest, malignant hyperthermia, hemorrhage, blood transfusion, and postoperative nausea/vomiting. Discharge status and unexpected medical return visits were tabulated. Complication rates for patients younger than 3 years were compared to the remaining cohort.Results:An estimated 1,410,546 ± 246,009 (± standard error) children underwent one or more pediatric otolaryngologic procedures in 2006 (mean age, 6.23 ± 0.19 years; 50.9% ± 2.2% male). The most common primary procedures performed were myringotomy and tube (551,222 cases), tonsillectomy with adenoidectomy (480,343 cases), adenoidectomy (81,048 cases), tonsillectomy (56,606 cases), myringotomy alone (27,691 cases), tympanostomy tube removal (21,446 cases), and close reduction of nasal fracture (15,230 cases). There were no reported cases of cardiac arrest, malignant hyperthermia, or blood transfusion. Postoperative nausea, vomiting, and perioperative bleeding occurred in 1.4%, 0.9%, and 0.2% of cases, respectively. Children younger than 4 years undergoing tonsillectomy were more likely to return to the surgery center (2.5%, P < .001) or have an unplanned admission (9.28%, P = .011) than older children.Conclusions:Pediatric otolaryngologic surgery is not only common but very safe in the ambulatory setting, with relatively minor complications occurring at rates of approximating 1%. Life‐threatening complications are exceedingly rare. Ambulatory tonsillectomy in children younger than 4 years requires careful selection. Laryngoscope, 2010
Miller fisher syndrome presents as an acute voice change to hypernasal speech
Authors: Howell Rebecca J. , Davolos Alexandra G. , Clary Matthew S. , Frake Paul C. , Joshi Arjun S. , Chaboki Houtan 
Published: March 2010

AbstractThe authors describe a 38‐year‐old man who presented with hypernasality, perioral and acroparesthesia, dyspnea, and dysphagia. Further evaluation revealed a diagnosis of Miller‐Fisher syndrome (MFS). MFS is a variant of Guillain‐Barré syndrome previously described in neurology and critical care journals; however, there is a paucity of work concerning this disease in the otolaryngology literature. An acute change in voice usually occurs secondary to inflammatory processes as seen after intubation and infection, but can occur as part of a more complex disease entity such as Guillain‐Barré or Miller‐Fisher syndrome. As such, clinicians should consider this in their evaluation of rhinolalia aperta. Laryngoscope, 2010
Gray matter correlations of cognition in incident Parkinson's disease
Authors: Dalaker Turi O. , Zivadinov Robert , Larsen Jan P. , Beyer Mona K. , Cox Jennifer L. , Alves Guido , Bronnick Kolbjorn , Tysnes Ole‐Bjorn , Antulov Ronald , Dwyer Michael G. , Aarsland Dag 
Published: March 2010

AbstractWe aimed to investigate whether mild cognitive impairment (MCI) in Parkinson's disease (PD) is characterized by region‐specific gray matter (GM) atrophy and to explore correlations between GM and cognition in PD. Magnetic resonance images of 42 newly diagnosed PD patients (of which 11 had MCI) and 37 normal controls were analyzed using voxel‐based morphometry. Analyses comparing groups showed no regional atrophy, and in patients there were no significant correlations between cognitive domain test performance and GM loss. In conclusion, GM atrophy does not seem to be a major feature of cognitive dysfunction in incident PD. © 2010 Movement Disorder Society
Progression of Parkinson's disease as evaluated by Hoehn and Yahr stage transition times
Authors: Zhao Ying Jiao , Wee Hwee Lin , Chan Yiong‐Huak , Seah Soo Hoon , Au Wing Lok , Lau Puay Ngoh , Pica Emmanuel Camara , Li Shu Chuen , Luo Nan , Tan Louis C.S. 
Published: March 2010

AbstractThis study was carried out to evaluate progression in Parkinson's disease (PD) by analyzing time taken to transit from one Hoehn and Yahr (H&Y) stage to the next stage and to investigate the variables that would be associated with H&Y transition times using a large PD database that contained prospectively collected information. Data were obtained from the movement disorder database of the National Neuroscience Institute in Singapore. Kaplan‐Meier (KM) survival analysis was adopted to investigate the time taken to progress through various H&Y stages. Cox regression analysis was used to examine the association between the baseline variables at the entry point of each H&Y stage and the progression to the next stage. A total of 695 patients (mean age: 65.2, male: 57.3%) were studied. Using KM analysis, the median time taken to transit from H&Y stage 1 to 2, 2 to 2.5, 2.5 to 3 were 20, 62, and 25 months, respectively; whereas the median time taken to progress from stage 3 to 4 and 4 to 5 were 24 and 26 months, respectively. Cox regression analysis revealed that older age‐at‐diagnosis, longer PD duration, and higher Unified Parkinson's Disease Rating Scale (UPDRS) motor scores at baseline were associated with a significantly faster progression through various H&Y stages. Gender and ethnicity were not associated with disease progression. In conclusion, H&Y transition time is a useful measure of disease progression in PD and may be utilized in clinical studies evaluating therapeutic interventions and prognostic factors in PD. © 2010 Movement Disorder Society
Ancient representation of Meige's syndrome in the Moche culture in the pre‐Columbian Peru
Authors: Martinez‐Castrillo Juan Carlos , Mariscal Ana , Garcia‐Ruiz Pedro 
Published: March 2010

AbstractThe Moches were a pre‐Columbian culture from Peru, who had a fine ceramic technique and used to represent diseases. One example is the potter presented here which represents a man with a probable Meige's syndrome and may be the first artistic representation of this disease. © 2010 Movement Disorder Society.
Agreement between self‐reported and pharmacy data on medication use in the Northern Finland 1966 Birth Cohort
Authors: Haapea Marianne , Miettunen Jouko , Lindeman Sari , Joukamaa Matti , Koponen Hannu 
Published: March 2010

AbstractObjective: To compare self‐reported (SR) medication use and pharmacy data for major psychoactive medications and three classes of medications used for different indications, and to determine the socio‐economic factors associated with the congruence.Methods: Postal questionnaire data collected in 1997 were compared with the register of the Social Insurance Institution of Finland on the reimbursed prescriptions purchased during 1997. Altogether 7625 subjects were included in this study. Drugs were categorized according to the Anatomical Therapeutic Chemical (ATC) system.Results: Kappa values were 0.77, 0.68, 0.84, 0.92 and 0.55 for antipsychotics, antidepressants, antiepileptics, antidiabetics and beta‐blocking agents, respectively. Prevalence‐adjusted and bias‐adjusted kappa values were almost perfect (0.98–1.00). Reliability of antipsychotics use was better for married subjects than for those who were not married; and of antidepressants use for highly educated and married subjects than for those who were less educated and were not married. Altogether 414 (5.4%) responders and 285 (7.1%) non‐responders had used at least one of the selected medications.Conclusion: Agreement between the SR and pharmacy data was moderate for psychoactive medication use. Even though data collected by postal questionnaire may underestimate the prevalence of medication use due to non‐participation it can be assumed accurate enough for study purposes. Copyright © 2010 John Wiley & Sons, Ltd.
Standardized protocol for a depletion of intramyocellular lipids (IMCL)
Authors: Ith Michael , Huber Philipp M. , Egger Andrea , Schmid Jean‐Paul , Kreis Roland , Christ Emanuel , Boesch Chris 
Published: March 2010

AbstractIntramyocellular lipids (IMCL) are flexible fuel stores that are depleted by physical exercise and replenished by fat intake. IMCL or their degradation products are thought to interfere with insulin signaling thereby contributing to insulin resistance. From a practical point of view it is desirable to deplete IMCL prior to replenishing them. So far, it is not clear for how long and at which intensity subjects have to exercise in order to deplete IMCL. We therefore aimed at developing a standardized exercise protocol that is applicable to subjects over a broad range of exercise capacity and insulin sensitivity and allows measuring reliably reduced IMCL levels.Twelve male subjects, including four diabetes type 2 patients, with wide ranges of exercise capacity (VO2peak per total body weight 27.9–55.8 ml*kg−1*min−1), insulin sensitivity (glucose infusion rate per lean body mass 4.7–15.3 mg*min−1*kg−1), and BMI (21.7–31.5 kg*m−2), respectively, were enrolled. Using 1H magnetic resonance spectroscopy (1H‐MRS), IMCL was measured in m.tibialis anterior and m.vastus intermedius before and during a depletion protocol of a week, consisting of a moderate additional physical activity (1 h daily at 60% VO2peak) and modest low‐fat (10–15%) diet.Absolute IMCL‐levels were significantly reduced in both muscles during the first 3 days and stayed constant for the next 3 days of an identical diet/exercise‐scheme. These reduced IMCL levels were independent of insulin sensitivity, yet a tendency to lower depleted IMCL levels has been observed in subjects with higher VO2peak.The proposed protocol is feasible in subjects with large differences in exercise capacity, insulin sensitivity, and BMI, leading to reduced IMCL levels that neither depend on the exact duration of the depletion protocol nor on insulin sensitivity. This allows for a standardized preparation of IMCL levels either for correlation with other physiological parameters or for replenishment studies. Copyright © 2010 John Wiley & Sons, Ltd.
Leukocyte adhesion deficiency‐III in an African‐American patient
Authors: Sabnis Himalee , Kirpalani Anjali , Horan John , McDowall Alison , Svensson Lena , Cooley Anthony , Merck Tara , Jobe Shawn , Hogg Nancy , Briones Michael 
Published: March 2010

AbstractLeukocyte adhesion deficiency‐III (LAD‐III) is a rare disorder characterized by abnormal signaling to β integrins, leading to defective leukocyte adhesion and chemotaxis and platelet aggregation. Here we present the first case of an African‐American female infant with this disorder. She had history of multiple infections, bleeding, and leukocytosis since birth. She was successfully treated with allogeneic bone marrow transplant using a reduced intensity‐conditioning regimen. Mutations in KINDLIN‐3 have been described in LAD‐III but the mutations in KINDLIN‐3 in her case are unique. Pediatr Blood Cancer 2010;55:180–182. © 2010 Wiley‐Liss, Inc.