bluuebunny/crossref_metadata_2025_split huggingface dataset:
T cell‐intrinsic PKD3 fine‐tunes differentiation into CD8+ central memory T cells and CD8 single positive thymocyte development
Authors: Koutník Jiří , Peer Sebastian , Humer Dominik , Sumara Grzegorz , Leitges Michael , Baier Gottfried , Siegmund Kerstin 
Published: May 2024

AbstractMembers of the Protein kinases D (PKD) family are described as regulators of T cell responses. From the two T cell‐expressed isoforms PKD2 and PKD3, so far mainly the former was thoroughly investigated and is well understood. Recently, we have investigated also PKD3 using conventional as well as conditional T cell‐specific knockout models. These studies suggested PKD3 to be a T cell‐extrinsic regulator of the cells' fate. However, these former model systems did not take into account possible redundancies with the highly homologous PKD2. To overcome this issue and thus properly unravel PKD3's T cell‐intrinsic functions, here we additionally used a mouse model overexpressing a constitutively active isoform of PKD3 specifically in the T cell compartment. These transgenic mice showed a slightly higher proportion of central memory T cells in secondary lymphoid organs and blood. This effect could not be explained via differences upon polyclonal stimulation in vitro, however, may be connected to the observed developmental aberrances in the CD8 single positive compartment during thymic development. Lastly, the observed alterations in the CD8+ T cell compartment did not impact proper immune response upon immunization with ovalbumin or in a subcutaneous tumour model suggesting only a small to absent biological relevance. Taking together the knowledge of all our published studies on PKD3 in the T cell compartment, we now conclude that T cell‐intrinsic PKD3 is a fine‐tuner of central memory T cell as well as CD8 single positive thymocyte development.
Developing the self‐determination of children and young people with intellectual disabilities in schools: A systematic review of the literature
Authors: Willow Sierra Angelina , Strnadová Iva , Danker Joanne 
Published: May 2024

AbstractBackgroundSelf‐determination is associated with lifelong positive outcomes. Students with intellectual disabilities typically have lower self‐determination than their peers. Universal basic education access offers schools the opportunity to rectify this disparity. This is the first systematic review investigating the school‐based practices that target self‐determination development for students with intellectual disabilities.MethodThe review follows the PRISMA guidelines, spanning five databases (ProQuest databases, EMBASE, Scopus, Sage Journals, Taylor and Francis Online) from 2006 to 2021.ResultsAcross the 18 studies, the most used practice is the SDLMI. Research focuses on United States‐based transition‐aged students with mild to moderate intellectual disabilities. Social validity tends to be assessed in summative and informal ways. Students are not generally involved in decision‐making about practices and individualisation of support.ConclusionsSelf‐determination development for this population can begin before puberty. Future research should critically investigate social validity and holistic integration of student self‐determination learning opportunities throughout the pedagogical cycle.
Lithium prevents glucocorticoid‐induced osteonecrosis of the femoral head by regulating autophagy
Authors: Wang Qiuru , Yang Zhouyuan , Li Qianhao , Zhang Wanli , Kang Pengde 
Published: May 2024

AbstractAutophagy may play an important role in the occurrence and development of glucocorticoid‐induced osteonecrosis of the femoral head (GC‐ONFH). Lithium is a classical autophagy regulator, and lithium can also activate osteogenic pathways, making it a highly promising therapeutic agent for GC‐ONFH. We aimed to evaluate the potential therapeutic effect of lithium on GC‐ONFH. For in vitro experiments, primary osteoblasts of rats were used for investigating the underlying mechanism of lithium's protective effect on GC‐induced autophagy levels and osteogenic activity dysfunction. For in vivo experiments, a rat model of GC‐ONFH was used for evaluating the therapeutic effect of oral lithium on GC‐ONFH and underlying mechanism. Findings demonstrated that GC over‐activated the autophagy of osteoblasts and reduced their osteogenic activity. Lithium reduced the over‐activated autophagy of GC‐treated osteoblasts through PI3K/AKT/mTOR signalling pathway and increased their osteogenic activity. Oral lithium reduced the osteonecrosis rates in a rat model of GC‐ONFH, and restrained the increased expression of autophagy related proteins in bone tissues through PI3K/AKT/mTOR signalling pathway. In conclusion, lithium can restrain over‐activated autophagy by activating PI3K/AKT/mTOR signalling pathway and up‐regulate the expression of genes for bone formation both in GC induced osteoblasts and in a rat model of GC‐ONFH. Lithium may be a promising therapeutic agent for GC‐ONFH. However, the role of autophagy in the pathogenesis of GC‐ONFH remains controversial. Studies are still needed to further explore the role of autophagy in the pathogenesis of GC‐ONFH, and the efficacy of lithium in the treatment of GC‐ONFH and its underlying mechanisms.
Molecular mechanisms of poor osseointegration in irradiated bone: In vivo study in a rat tibia model
Authors: Omar Omar , Rydén Louise , Wamied Abdel Rahman , Al‐Otain Ibrahim , Alhawaj Hussain , Abuohashish Hatem , Al‐Qarni Faisal , Emanuelsson Lena , Johansson Anna , Palmquist Anders , Thomsen Peter 
Published: May 2024

AbstractAimRadiotherapy is associated with cell depletion and loss of blood supply, which are linked to compromised bone healing. However, the molecular events underlying these effects at the tissue–implant interface have not been fully elucidated. This study aimed to determine the major molecular mediators associated with compromised osseointegration due to previous exposure to radiation.Materials and MethodsTitanium implants were placed in rat tibiae with or without pre‐exposure to 20 Gy irradiation. Histomorphometric, biomechanical, quantitative polymerase chain reaction (qPCR) and enzyme‐linked immunosorbent assay analyses were performed at 1 and 4 weeks after implantation.ResultsThe detrimental effects of irradiation were characterized by reduced bone–implant contact and removal torque. Furthermore, pre‐exposure to radiation induced different molecular dysfunctions such as (i) increased expression of pro‐inflammatory (Tnf) and osteoclastic (Ctsk) genes and decreased expression of the bone formation (Alpl) gene in implant‐adherent cells; (ii) increased expression of bone formation (Alpl and Bglap) genes in peri‐implant bone; and (iii) increased expression of pro‐inflammatory (Tnf) and pro‐fibrotic (Tgfb1) genes in peri‐implant soft tissue. The serum levels of pro‐inflammatory, bone formation and bone resorption proteins were greater in the irradiated rats.ConclusionsIrradiation causes the dysregulation of multiple biological activities, among which perturbed inflammation seems to play a common role in hindering osseointegration.
The contributions of grandparents to preadolescent grandchildren's social skills in rural Malaysia
Authors: Tan Jo‐Pei , Gan Su‐Wan , Cheah Charissa S. L. , Tan Soon‐Aun , Nainee Sarvarubini , Yap Chin Choo , Hon Kai Yee 
Published: May 2024

AbstractThis study aimed to generate localized knowledge by investigating the perceptions and experiences of preadolescent grandchildren and grandparents regarding grandparenting and intergenerational interactions and how these processes were related to the social skills of preadolescents from three ethnic groups in Malaysia. Using a concurrent quantitative–qualitative mixed method research design, Chinese, Malay, and Indian preadolescents (N = 465; ages 9–12 years old; M = 10.27 years; SD = 1.03) from rural areas in Malaysia completed a self‐administered quantitative survey; furthermore, 25 grandparents participated in one‐on‐one interviews. Survey findings showed that preadolescent grandchildren who reported higher grandparental warmth and support had greater social skills, mediated by positive grandparent–grandchildren (GP–GC) relationships. The GP–GC relationship and preadolescent social skills association was stronger for skipped generation compared to three‐generation households. Interview findings revealed that grandparents expressed unconditional love and autonomy support in their grandparenting roles by guiding and encouraging their preadolescent grandchildren to make decisions. The GP–GC interactions served as a dynamic force in promoting preadolescents' social skills. By employing a decolonized approach and drawing on the lived experiences of grandparents from three ethnic backgrounds in rural Malaysia, the study provided an understanding of grandparenting practices and their general implications across the three ethnic groups. The interview responses highlighted both commonalities and specificities in grandparenting practices and relationship dynamics shaped by religious, class, and sociocultural dimensions in rural Malaysia.
Kierkegaard, Lippmann, and the Phantom Public in a Digital Age
Authors: Haman John P. 
Published: May 2024

ABSTRACTSøren Kierkegaard and Walter Lippmann wrote in very different times and places but both characterized the public as a “phantom.” Importantly, each did so within the context of a broader analysis that linked the press with specific notions about the public and democracy. This paper highlights the specific characteristics of the press that each thinker believed were responsible for the construction of the phantom public and its effects. While taking seriously the differences between Kierkegaard and Lippmann, in both their respective sociopolitical contexts and their formulations of the public and the press, this paper applies their critiques to a vastly different media terrain than either thinker could have envisioned.
Perceptions and experiences of UK‐based mothers of autistic daughters in relation to the potential affordances and constraints of an autism diagnosis
Authors: Evans Mairi , Papadopoulos Chris , Burnham John 
Published: May 2024

AbstractWhilst there is a growing body of research about autistic girls, much less is known about the experience of mothering an autistic girl, and the potential impact of the diagnosis. This study qualitatively explored the diagnostic journey of 12 mothers in the UK to identify the meanings attributed to their daughter's diagnosis. A thematic analysis was applied with themes examined through the lens of the CMM LUUUUTT model to further explore the stories lived and told by the mothers. Eighteen themes linked to perceived affordances, perceived constraints/constraints of perception, experienced affordances and experienced constraints. Participants highlighted the impact of autism myths and stereotypes which influenced identification, referral, diagnosis and ongoing support for the girls. Myths and stereotypes told about autism also played a significant role in the mothers lived experience of the diagnosis. An affordance of diagnosis was a new understanding about their daughters' needs, which led to new parenting styles, letting go of blame and resisting perceived societal ‘oughtisms’ about how parenting should be. Whilst the diagnosis was seen as relationally transformative, challenges were described in accessing emotional or educational support for their daughter's post‐assessment.
Media discourses of migration: A focus on Europe
Authors: Fuller Janet M. 
Published: May 2024

AbstractWith a focus on the post‐2015 period in the western and northern regions of Europe, the research examined here shows prominent media discourses of othering, threat and deservedness of migrants. This spatial and temporal frame lends itself to the study of how discourses reflect the impact of the so‐called ‘refugee crisis’ in these regions of Europe. Since there was also continued immigration related to increased opportunities for work, education, quality of life and family togetherness which have long brought migrants to these European countries, examining the research in this period allows us to discover how these discourses might distinguish between different migrant experiences. There is some evidence for the differentiation of certain types of people of migration background in the media discourses, despite a strong tendency to stereotype and essentialise regardless of the actual background of migrants or their descendants. Another key aspect in the research to date is how professional versus participatory media can be compared in the discourses of migration they reproduce, and how these different types of media play a role in society. The article ends with a call for a more intersectional perspective on migration which incorporates critical perspectives on racialisation, and further examination of the voices of migrants in the media.
Symptoms from the Gastroparesis Cardinal Symptom Index and clinical factors associated with delayed gastric emptying in patients with suspected gastroparesis
Authors: Sabat Claire , Lebtahi Rachida , Duboc Henri , Dior Marie , Coffin Benoit , Soliman Heithem 
Published: May 2024

AbstractBackgroundThe association between upper gastrointestinal symptoms and delayed gastric emptying (GE) shows conflicting results. This study aimed to assess whether the symptoms of the Gastroparesis Cardinal Symptom Index (GCSI) and/or the scores were associated with the result of GE tests and whether they could predict delayed GE.MethodsPatients referred for suspected gastroparesis (GP) were included in a prospective database. Demographical data, medical history, and symptoms of the GCSI score were collected for each patient. A GE scintigraphy was then performed with a 4‐hour recording. Delayed GE was defined as a retention rate ≥ 10% at 4 h.ResultsAmong 243 patients included in this study, 110 patients (45%) had delayed GE. The mean age (49.9 vs. 41.3 years; p < 0.001) and weight loss (9.4 kg vs. 5.6 kg; p = 0.025) were significantly higher in patients with delayed GE. Patients with diabetes or a history of surgery had a higher prevalence of delayed GE (60% and 78%, respectively) than patients without comorbidity (17%; p < 0.001). The GCSI score was higher in patients with delayed GE (3.06 vs. 2.80; p = 0.045), but no threshold was clinically relevant to discriminate between patients with normal and delayed GE. Only vomiting severity was significantly higher in patients with delayed GE (2.19 vs. 1.57; p = 0.01).ConclusionGE testing should be considered when there are symptoms such as a higher weight loss, comorbidities (diabetes, and history of surgery associated with GP), and the presence of vomiting. Other symptoms and the GCSI score are not useful in predicting delayed GE.
INJEX50 could improve the success rate of local anesthesia for arterial cannulation in the pediatric intensive care unit: A randomized, double‐blind, single‐center study
Authors: Sakai Wataru , Chaki Tomohiro , Tachibana Shunsuke , Ichisaka Yuki , Nawa Yuko , Nawa Tomohiro , Yamakage Michiaki 
Published: May 2024

AbstractBackgroundQuick arterial cannulation is required in pediatric emergency situation, which require effective local anesthesia to avoid withdrawal movement. However, pediatric local anesthesia could be difficult because of withdrawal movement. Jet injectors, which are needleless and provide local anesthesia quickly, could be helpful for pediatric local anesthesia during arterial cannulation.AimsThis study aimed to examine whether new jet injector “INJEX50” could improve the success rate of local anesthesia for arterial cannulation in pediatric intensive care unit compared with the current standard of care, infiltration using a 26‐gauge needle.MethodsThis study was a randomized, double‐blind, single‐center study. Participants were infants and young children in the pediatric intensive care unit, who required an arterial line. Local anesthesia was performed with either a 26‐gauge needle (group C) or INJEX50 (group I) before arterial cannulation. The primary outcome (success of local anesthesia) was the presence of withdrawal movement at the time of skin puncture for arterial cannulation. The secondary outcomes included rescue sedation during arterial cannulation. Data were analyzed using Fisher's exact test and the Mann–Whitney U‐test, with values of p < .05 considered statistically significant.ResultsSeventy patients were randomly assigned to groups C and I. The local anesthesia success rate in group I (30/35 [86%]) was significantly higher than that in group C (15/35 [43%], odds ratio, 8.00; 95% confidence interval, 2.51–25.5; p = .0005). In conclusion, INJEX50 could improve success rate of local anesthesia for arterial cannulation in pediatric intensive care unit compared with 26‐gauge needle.
Acinetobacter in fresh faecal samples is related to sperm motility: A case report in Piétrain boars housed on different deep‐litter bedding materials
Authors: Riedel Anine , Vahjen Wilfried , Lautner Matthias , Leiding Claus , Jung Markus , Zentek Jürgen , Schulze Martin 
Published: May 2024

AbstractThe swine gastrointestinal tract contains a great variety of microbes, forming a complex and dynamic ecosystem. Various internal and external factors (e.g. age, breed and diet) may influence its composition. This study aimed to investigate the gut microbial diversity of German Piétrain boars housed on different deep‐litter bedding materials (regional wood shavings, linen, hemp, spelt husks, and wood shavings) via 16S‐rDNA sequencing. Additionally, short‐chain fatty acids were analysed using gas chromatography. Fresh faecal samples (n = 80) from 40 Piétrain boars were collected twice during the trial. Although it can be assumed that boars ingest bedding orally, no differences in the microbiome composition could be found. The main phyla were Firmicutes and Bacteroides. Acinetobacter was identified as a biomarker for sperm quality differences (total sperm motility) in breeding boars.
Adjusting ram semen preservation: Exploring the impact of oxygen exposure during liquid storage
Authors: Ben Moula Anass , Hamidallah Naima , Badi Abdelmoughit , El Fadili Moussa , El Amiri Bouchra 
Published: May 2024

AbstractThis study investigated the effects of storage conditions on the quality of chilled ram semen stored at 4°C for 48 h, comparing aerobic and anaerobic conditions. Ejaculates from INRA180 rams were collected and stored under both conditions, with assessments at 0‐, 24‐, and 48‐h intervals. Various sperm parameters were examined, including motility, velocity, viability, morphology, membrane integrity, and lipid peroxidation. Results showed that storage duration significantly impacted sperm quality, leading to a gradual decline from 0 to 24 h and 24 to 48 h. Notably, after the initial 24 h, progressive motility (PM) and membrane integrity (MI) demonstrated distinct responses to storage conditions. Anaerobic storage consistently improved PM and MI values compared to aerobic storage between 24 and 48 h. Anaerobic conditions also enhanced viability and reduced abnormality at the 48‐h mark. Total motility remained stable throughout storage. Velocity parameters (VCL: curvilinear velocity; VSL: straight velocity and VAP: velocity average path) exhibited differences between the 24‐ and 48‐h intervals, with anaerobic storage resulting in higher VAP and VSL values. Moreover, lipid peroxidation exhibited a progressive increase from 0 to 24 h and 24 to 48 h, independent of storage conditions. Remarkably, anaerobic storage consistently yielded lower lipid peroxidation levels compared to aerobic storage, regardless of storage duration. In conclusion, this study highlights that the anaerobic storage proved advantageous for chilled ram semen quality, particularly after the initial 24 h.
Dynamics of transcription is affected by oxygen tension and developmental speed during in vitro production of bovine embryos
Authors: de Lima Camila Bruna , do Amaral Danilo Trabuco , Ispada Jéssica , dos Santos Érika Cristina , Fontes Patrícia Kubo , Nogueira Marcelo Fábio Gouveia , Milazzotto Marcella Pecora 
Published: May 2024

AbstractThis study examines the impact of oxygen tension and embryo kinetics on gene transcription dynamics in pathways crucial for embryonic preimplantation development, including lipid metabolism, carbohydrate transport and metabolism, mitochondrial function, stress response, apoptosis and transcription regulation. Bovine embryos were generated in vitro and allocated into two groups based on oxygen tension (20% or 5%) at 18 h post insemination (hpi). At 40 hpi, embryos were categorized into Fast (≥4 cells) or Slow (2 cells) groups, resulting in four experimental groups: FCL20, FCL5, SCL20 and SCL5. Embryo collection also occurred at 72 hpi (16‐cell stage; groups FMO20, FMO5, SMO20 and SMO5) and at 168 hpi (expanded blastocyst (BL) stage; groups FBL20, FBL5, SBL20 and SBL5). Pools of three embryos per group were analysed in four replicates using inventoried TaqMan assays specific for Bos taurus, targeting 93 genes. Gene expression patterns were analysed using the K‐means algorithm, revealing three main clusters: genes with low relative abundance at the cleavage (CL) and 16‐cell morula (MO) stages but increased at the BL stage (cluster 1); genes with higher abundances at CL but decreasing at MO and BL (cluster 2); and genes with low levels at CL, higher levels at MO and decreased levels at BL (cluster 3). Within each cluster, genes related to epigenetic mechanisms, cell differentiation events and glucose metabolism were particularly influenced by differences in developmental kinetics and oxygen tension. Fast‐developing embryos, particularly those cultured under low oxygen tension, exhibited transcript dynamics more closely resembling that reported in vivo‐produced embryos.
Everyday acts of kindness predict greater well‐being during the transition to university
Authors: Cash Tiara A. , Aknin Lara B. , Girme Yuthika U. 
Published: May 2024

AbstractFrom becoming a teenager to starting university, life transitions are an inevitable part of human existence. While exciting, life transitions can be stressful because they involve changes in identity, routine, and expectations. What can support people during this period of change? Informed by past research demonstrating the emotional benefits of prosocial behavior, we examined whether everyday acts of prosociality might predict well‐being during a life transition using a pre‐registered 6‐week diary study conducted with students starting university (N = 193; 1544 observations). Consistent with pre‐registered hypotheses, participants experienced higher well‐being on scales capturing happiness, flourishing, thriving, optimism, resilience, anxiety and loneliness during weeks in which they completed more prosocial acts than their personal average. This research extends our understanding of the relationship between prosociality and well‐being to new theoretically relevant contexts, including extended, multi‐faceted personal stressors, and suggests that one potentially useful route to well‐being during life transitions could be helping others.