bluuebunny/crossref_metadata_2025_split huggingface dataset:
NBR1-mediated selective autophagy of ARF7 modulates root branching
Authors: Ebstrup Elise , Ansbøl Jeppe , Paez-Garcia Ana , Culp Henry , Chevalier Jonathan , Clemmens Pauline , Coll Núria S , Moreno-Risueno Miguel A , Rodriguez Eleazar 
Published: April 2024

AbstractAuxin dictates root architecture via the Auxin Response Factor (ARF) family of transcription factors, which control lateral root (LR) formation. In Arabidopsis, ARF7 regulates the specification of prebranch sites (PBS) generating LRs through gene expression oscillations and plays a pivotal role during LR initiation. Despite the importance of ARF7 in this process, there is a surprising lack of knowledge about how ARF7 turnover is regulated and how this impacts root architecture. Here, we show that ARF7 accumulates in autophagy mutants and is degraded through NBR1-dependent selective autophagy. We demonstrate that the previously reported rhythmic changes to ARF7 abundance in roots are modulated via autophagy and might occur in other tissues. In addition, we show that the level of co-localization between ARF7 and autophagy markers oscillates and can be modulated by auxin to trigger ARF7 turnover. Furthermore, we observe that autophagy impairment prevents ARF7 oscillation and reduces both PBS establishment and LR formation. In conclusion, we report a novel role for autophagy during development, namely by enacting auxin-induced selective degradation of ARF7 to optimize periodic root branching.
GP64-pseudotyped lentiviral vectors target liver endothelial cells and correct hemophilia A mice
Authors: Milani Michela , Canepari Cesare , Assanelli Simone , Merlin Simone , Borroni Ester , Starinieri Francesco , Biffi Mauro , Russo Fabio , Fabiano Anna , Zambroni Desirèe , Annoni Andrea , Naldini Luigi , Follenzi Antonia , Cantore Alessio 
Published: April 2024

AbstractLentiviral vectors (LV) are efficient vehicles for in vivo gene delivery to the liver. LV integration into the chromatin of target cells ensures their transmission upon proliferation, thus allowing potentially life-long gene therapy following a single administration, even to young individuals. The glycoprotein of the vesicular stomatitis virus (VSV.G) is widely used to pseudotype LV, as it confers broad tropism and high stability. The baculovirus-derived GP64 envelope protein has been proposed as an alternative for in vivo liver-directed gene therapy. Here, we perform a detailed comparison of VSV.G- and GP64-pseudotyped LV in vitro and in vivo. We report that VSV.G-LV transduced hepatocytes better than GP64-LV, however the latter showed improved transduction of liver sinusoidal endothelial cells (LSEC). Combining GP64-pseudotyping with the high surface content of the phagocytosis inhibitor CD47 further enhanced LSEC transduction. Coagulation factor VIII (FVIII), the gene mutated in hemophilia A, is naturally expressed by LSEC, thus we exploited GP64-LV to deliver a FVIII transgene under the control of the endogenous FVIII promoter and achieved therapeutic amounts of FVIII and correction of hemophilia A mice.
New quasars behind the Magellanic Clouds
Authors: Ivanov Valentin D. , Cioni Maria-Rosa L. , Dennefeld Michel , de Grijs Richard , Craig Jessica E. M. , van Loon Jacco Th. , Pennock Clara M. , Maitra Chandreyee , Haberl Frank 
Published: April 2024

Context. Quasi-stellar objects (QSOs) are a basis for an absolute reference system for astrometric studies. A system like this at the far side of nearby galaxies is required to facilitate measuring of the proper motions of these galaxies. However, the foreground contamination from the galaxies themselves is a problem for the QSO identification.
Aims. We search for new QSOs behind the two Magellanic Clouds, the Magellanic Bridge, and the Magellanic Stream.
Methods. We identify QSO candidates with a combination of near–infrared colors and variability criteria from the public ESO Visual and Infrared Survey Telescope for Astronomy (VISTA) Magellanic Clouds (VMC) survey. We confirm their nature from broad emission lines with low-resolution optical spectroscopy.
Results. We confirmed the QSO nature of 136 objects. They are distributed as follows: 12 behind the Large Magellanic Cloud, 37 behind the Small Magellanic Cloud, 63 behind the Bridge, and 24 behind the Stream. The QSOs span a redshift range from z~0.1 to z~2.9. A comparison of our quasar selection with the Quaia quasar catalog, based on Gaia low-resolution spectra, yields a selection and confirmation success rate of 6–19%, depending on whether the quality of the photometry, the magnitude ranges, and the colors are considered. Our candidate list is rather incomplete, but the objects in it are likely to be confirmed as quasars with a probability of ~90%. Finally, we report a list of 3609 objects in the entire VMC survey that match our color and variability selection criteria; only 1249 of them have Gaia counterparts.
Conclusions. Our combined infrared color and variability criteria for the QSO selection prove to be efficient: ~90% of the observed candidates are bona fide QSOs and allow us to generate a list of new high-probability quasar candidates.
The longevity of the oldest open clusters
Authors: Alvarez-Baena N. , Carrera R. , Thompson H. , Balaguer-Nuñez L. , Bragaglia A. , Jordi C. , Silva-Villa E. , Vallenari A. 
Published: April 2024

Context.The dynamical evolution of open clusters is driven by stellar evolution, internal dynamics, and external forces, which according to dynamical simulations will lead to their evaporation over a timescale of about 1 Ga. However, about 10% of the known open clusters are older. These latter are special systems whose detailed properties are related to their dynamical evolution and the balance between mechanisms of cluster formation and dissolution.Aims.We investigated the spatial distribution and structural parameters of six open clusters older than 1 Ga in order to constrain their dynamical evolution and longevity.Methods.We identified members usingGaiaEDR3 data up to a distance of 150 pc from the centre of each cluster. We investigated the spatial distribution of stars inside each cluster to understand their degree of mass segregation. Finally, in order to interpret the obtained radial density profiles, we reproduced them using the lowered isothermal model explorer withPYthon(LIMEPY) and the spherical potential escapers stitched (SPES) models.Results.All the studied clusters appear to be more extended than previously reported in the literature. The spatial distributions of three of them show some structures aligned with their orbits. These structures may be related to the existence of extra tidal stars. Moreover, we find that about 20% of their members have sufficient energy to leave the systems or are already unbound. Together with their initial masses, their distances to the Galactic plane may play significant roles in their survival. We find clear evidence that the most dynamically evolved clusters do not fill their Roche volumes, appearing more concentrated than the others. Finally, we find a cusp–core dichotomy in the central regions of the studied clusters, which shows some similarities to that observed among globular clusters.
From VIPERS to SDSS: Unveiling galaxy spectra evolution over 9 Gyr through unsupervised machine learning
Authors: Dubois J. , Siudek M. , Fraix-Burnet D. , Moultaka J. 
Published: April 2024

Aims. This study aims to trace the chronological evolution of galaxy spectra over cosmic time. Focusing on the VIPERS dataset, we seek to understand the diverse population of galaxies within narrow redshift bins, comparing our findings with the previously mapped diversity of SDSS galaxies.
Methods. We used Fisher-EM, an unsupervised sub-space model-based classification algorithm to classify a dataset of 79 224 galaxy spectra from the VIMOS Public Extragalactic Redshift Survey (VIPERS). The dataset was divided into 26 samples by bins of redshift ranging from z ∼ 0.4 to z ∼ 1.2, which were classified independently. Classes of subsequent bins were linked through the k-nearest neighbours method to create a chronological tree of classes at different epochs.
Results. Based on the optical spectra, three main chronological galaxy branches emerged: (i) red passive, (ii) blue star forming, and (iii) very blue, possibly associated with AGN activity. Each of the branches differentiates into sub-branches, discriminating finer properties such as D4000 break, colour, star-formation rate, and stellar masses, and/or disappear with cosmic time. Notably, these classes align remarkably well with the branches identified in a previous SDSS analyses, indicating a robust and consistent classification across datasets. The chronological ‘tree’ constructed from VIPERS data provides valuable insights into the temporal evolution of these spectral classes.
Conclusions. The synergy between VIPERS and SDSS datasets enhances our understanding of the evolutionary pathways of galaxy spectra. The remarkable correspondence between independently derived branches in both datasets underscores the reliability of our unsupervised machine-learning approach. The three sub-trees show complex branching structures that highlight different physical and evolutionary behaviours. This study contributes to the broader comprehension of galaxy evolution by providing a chronologically organised framework for interpreting optical spectra within specific redshift ranges.
Management von Verletzungen parenchymatöser Abdominalorgane
Authors: Schild-Suhren Stina , Yilmaz Elif , Biggemann Lorenz , Seif Ali , Torsello Giovanni Federico , Uhlig Annemarie , Ghadimi Michael , Bösch Florian 
Published: April 2024

ZusammenfassungDie häufigsten betroffenen Organe abdomineller Traumata sind die Milz und die Leber, häufig auch in Kombination. Verletzungen des Pankreas sind vergleichsweise selten. Beim deutlich häufigeren stumpfen Abdominaltrauma sollte nach Einschätzung der Kreislaufsituation eine klinische und laborchemische Untersuchung sowie eine Sonografie durchgeführt werden. Sofern eine hämodynamische Instabilität und freie Flüssigkeit vorliegen, ergibt sich die Indikation zur notfallmäßigen Laparotomie. Bei stabiler bzw. stabilisierbarer Situation und auffälliger Sonografie sowie obligat beim polytraumatisierten Patienten ist die Durchführung einer triphasischen Kontrastmittel-Computertomografie unabdingbar. Bei Verdacht auf eine renale Verletzung sollte eine spätvenöse urografische Phase angeschlossen werden. Neben der dadurch möglichen Klassifizierung der Verletzung ist auf eine mögliche Gefäßbeteiligung oder akute Blutung zu achten. Sollte Letzteres vorliegen, ist eine Angiografie mit
Interventionsmöglichkeit anzustreben. Bei Verletzungen des Ductus pancreaticus ist eine endoskopische Behandlung möglich. Sollte sich in der Bildgebung kein Interventionsziel demaskieren und eine stabile Kreislaufsituation vorherrschen, ist ein rein konservatives Vorgehen unter kontinuierlicher Monitorüberwachung mittels klinischer, laborchemischer und sonografischer Kontrollen möglich. Die meisten Verletzungen können erfolgreich konservativ (nicht operatives Management [NOM]) behandelt werden.Das operative Verfahren ergibt sich aus dem Verletzungsmuster und reicht von der lokalen Blutstillung bis zur Organresektion. Ebenso besteht die Option der „Damage Control Surgery“ mit akuter Blutungskontrolle und zweizeitiger Versorgung. Komplexe chirurgische Eingriffe sollten an Zentren durchgeführt werden. Postoperative Komplikationen ergeben sich analog der elektiven Chirurgie.Beim selteneren penetrierenden Bauchtrauma kann das tatsächliche Verletzungsausmaß nicht durch die äußerlich sichtbare Wunde eingeschätzt werden. Auch hier ist primär die Kreislaufsituation entscheidend und bei Instabilität eine Notfalllaparotomie durchzuführen. Bei Stabilität sollte eine weitere Diagnostik mittels Kontrastmittel-Computertomografie erfolgen. Bei nicht klar auszuschließender Penetration des Peritoneums ist eine diagnostische Laparoskopie indiziert.
Diagnostic Value of Stimulated Urine Luteinizing Hormone After
Triptorelin Stimulation Test in Girls with Central Precocious
Puberty
Authors: Jia Ruofan , Xu Zhuangjian , Zhou Yuan , Zeng Beilei , Chen Chunmei , Huang Panwang , Ren Feng , Kong Fan-sheng , Ma Yaping 
Published: April 2024

Abstract
Objective To investigate the diagnostic value of urine luteinizing hormone
(ULH) after the triptorelin stimulation test detected by immunochemiluminometric
assay (ICMA) in girls with central precocious puberty (CPP).
Methods The girls with precocious puberty were included. The triptorelin
stimulation test at 8:30 a.m. was performed. Two consecutive 12-hour urine
samples were collected after the test, defined as the first 12-hour and second
12-hour urine, respectively. ICMA measured ULH. Urine creatinine (Cr)
concentration was measured. CPP and peripheral precocious puberty (PPP) were
diagnosed by the same pediatric endocrinologist based on clinical symptoms,
signs, and progression of clinical development.
Results A total of 97 cases (CPP n=69; PPP n=28) were included, with 12
cases not meeting the receiver operating characteristic analysis criteria. The
first and second 12-hour ULH/Cr in the CPP group were higher than those in the
PPP group. When the first 12-hour ULH/Cr was≥287.252 IU/mol, the sensitivity and
specificity for diagnosing CPP were 87.3% and 90.9%, respectively. When the
second 12-hour ULH/Cr was≥152.769 IU/mol, the sensitivity and specificity for
diagnosing CPP were 92.1% and 90.9%, respectively. The area under the curve of
the first and second 12-hour ULH/Cr were 0.933 and 0.954, respectively.
Conclusion The ULH detection method after the triptorelin stimulation test
has clinical significance for diagnosing CPP in girls. When blood sampling
compliance in girls with precocious puberty is poor, the first 12-hour
ULH/Cr≥288 IU/mol (or second 12-hour≥153 IU/mol) after the triptorelin
stimulation test can serve as a laboratory indicator for diagnosis of CPP.
One-Pot Expeditious Synthesis of Pyrazoloindolones via Base-Promoted Electrocyclization, C–N Coupling and Intramolecular Oxidative Cyclization
Authors: Singh Maya Shankar , Basak Ashok Kumar , Korawat Harshita Singh , Saini Manoj Kumar , Prajapati Karmdeo 
Published: April 2024

AbstractPyrazoloindolones are synthesized from N-tosylhydrazones in a one-pot multistep process which include base-promoted (i) electrocyclization reaction of N-tosylhydrazones derived from α,β-unsaturated aldehydes, (ii) aromatic nucleophilic substitution, and (iii) a domino cyclization–oxidation process under aerobic conditions.
Outcomes in colorectal endoscopic submucosal dissection for large protruded lesions: A retrospective multicenter study
Authors: Chiba Hideyuki , Ohata Ken , Hayashi Akimichi , Ebisawa Yu , Kobayashi Mikio , Arimoto Jun , Kuwabara Hiroki , Minato Yohei , Nakaoka Michiko 
Published: April 2024

Abstract
Background and study aims Colorectal endoscopic submucosal dissection (ESD) is increasingly used for treating early-stage colorectal cancer, including large, protruded lesions (LPL). However, the challenges posed by LPLs, especially those accompanied by severe fibrosis or muscle-retracting sign (MRS), remain unclear. This study aims to investigate ESD outcomes for LPL, focusing on factors such as tumor size and, submucosal fibrosis.
Patients and methods In a multicenter retrospective study (June 2012 to May 2023), data from 526 patients with 542 LPL lesions (≥ 2 cm) were analyzed. Parameters included lesion size, procedure time, dissection speed, physician experience, submucosal fibrosis, and adverse events. The tunnel method, including the double tunnel method, was used for cases with severe fibrosis or MRS. Multivariate analysis assessed factors affecting procedure difficulty, particularly LPLs ≥ 4 cm.
Results The study revealed an impressive en bloc resection rate of 97.8% and a curative resection rate of 78.6% for LPLs. Notably, fibrosis and MRS were present in 25% and 18% of 4-cm LPLs, respectively, and their frequency tended to increase as the tumor diameter increased. One treatment strategy for LPLs was the tunneling method, which was used most frequently (41 cases, 7.6%). Factors affecting dissection speed included larger tumor size, submucosal fibrosis, MRS, and physician experience.
Conclusions Treating LPLs through colorectal ESD presents significant challenges, especially in patients with fibrosis and MRS. This study highlights the importance of recognizing these complexities, and that more reliable resection strategy must be established for accurate pathological evaluation
Evaluation of Admission Brain Computed Tomography Findings to Predict the Long-term Outcomes of Patients with Traumatic Brain Injury
Authors: Dantas Rafael de Souza , Melo Thais Cristina de Souza , Lins Isabella Fontes de Santana , Santos Letícia Adrielle dos , Carvalho Neto José Nolasco de , Santos Bruno Fernandes de Oliveira , Amorim Robson Luis Oliveira de , Oliveira Arthur Maynart Pereira 
Published: April 2024

Abstract
Objective To evaluate the admission brain computed tomography (CT) scan findings in patients with traumatic brain injury (TBI) in a low- and middle-income country (LMIC) to predict long-term neurological outcomes.
Materials and Methods Patients admitted to a tertiary emergency hospital between March 2017 and April 2018 who had suffered a TBI and had undergone a brain CT scan within 12 hours of the trauma were prospectively evaluated. All of the patients who were hospitalized for at least 24 hours were contacted by phone after 12 months to evaluate their neurological condition.
Results We achieved a 12-month follow-up with 180 patients, most of them male (93.33%). The brain changes identified by CT, such as brain contusion (BC; p = 0.545), epidural hemorrhage (EDH; p = 0.968) and skull base fracture (SBF; p = 0.112) were not associated with worse neurological outcomes; however, subdural hemorrhage (SDH; p = 0.041), subarachnoid hemorrhage (SAH; p ≤ 0.001), brain swelling (BS; p ≤0.001), effacement of cortical sulci (ECS; p = 0.006), effacement of basal cisterns (EBC; p ≤0.001), depressed skull fracture (DSF; p = 0.017), and a brain midline shift > 5 mm (p = 0.028) were associated with worse outcomes.
Conclusion Findings such as SAH, BS and DSF were independent predictors of worse neurological outcomes. The rate of 70% of patients lost to follow-up shows the difficulties of conducting long-term research in LMICs.
Average Force Applied for Individual Tooth in All Ceramic Restoration
Authors: Halili Rajmonda , Shabani Alban , Asllani Lumturie , Alidema Sebahate Hamiti 
Published: April 2024

Abstract
Objectives Occlusion plays an important role in the retention and stability of dental restorations. Aim of this study was to analyze occlusal loading detected on dental elements in patients with unilateral partial edentulism—class III Kennedy, before and immediately after prosthetic treatment with a zirconia-based bridge in maximal intercuspation position with the help of electronic system T-scan III.
Materials and Methods The research sample consisted of a total of 60 patients who, based on the preset selection criteria, were divided into two groups: control group with 30 (100%) patients with intact dentition (Gr1) and study group with 30 (100%) patients with unilateral partial edentulism—class III Kennedy, analyzed before prosthetic treatment (Gr2) and immediately after prosthetic treatment with a zirconia-based bridge (Gr3).
Results In Gr1, the highest average force applied for individual tooth had the tooth numbers: 17–14.6 ± 9.9%, 26–11.9 ± 6.8%, and 27–11.7 ± 6.8%. The lowest average value was registered for the teeth number 22–1.4 ± 1.3%, 12–1.9 ± 3.8%, and 13–2.9 ± 2.7%. In the group Gr2, the highest average force applied for individual tooth had the tooth numbers: 17–12.9 ± 35.6 and 21–9.6 ± 13.9%, whereas the lowest in teeth 24–2.7 ± 5.2 and 27–3.4 ± 6.8. In Gr3, the highest average force applied for individual tooth had the tooth numbers: 17–11.8 ± 15.4 and 14–9.8 ± 10.9%, whereas the lowest in the tooth 22–2.1 ± 2.6.
Conclusion This study demonstrated that all patients with the loss of teeth showed occlusal discrepancies and functional disharmony. During the delivery of zirconia restorations in the future a careful analysis of occlusal contacts should be performed. T-scan III technology reduces the subjective interpretation of occlusal analysis data allowing records and measures the contacts between the teeth.
Management of Geographic Tongue with Individualized Homeopathy: An Evidence-Based Case Report
Authors: Saklani Nitin Kumar 
Published: April 2024

AbstractGeographic tongue, also known as benign migratory glossitis, is a common and benign condition characterized by distinct features on the tongue's surface. Typically, it affects the tip, lateral borders, and dorsum of the tongue, with occasional extension to the ventral surface. Indeed, geographic tongue commonly starts in childhood and has a global prevalence estimated at around 1 to 2.5% of the general population. In the pediatric population, the prevalence ranges from 0.37 to 14.3%, indicating that it can vary significantly among different age groups. The age group of 20 to 29 years seems to have the highest prevalence, with approximately 39.4% of individuals affected by geographic tongue. This suggests a peak incidence during early adulthood. This case report explores the potential benefits of homeopathic medicines in management of geographic tongue. A 17-year-old adolescent girl experiencing whitish discoloration of the tongue accompanied by intense burning sensation with history of recurrent tonsillitis and increased sweating in her palms and soles since early childhood presented in the OPD of Regional Research Institute for Homoeopathy, Shimla. Upon examination of her oral cavity, white patches were observed on her tongue, which did not resolve despite trying various medications. The primary diagnosis was geographic tongue, confirmed by physical examination. Following homeopathic treatment, the patient experienced marked improvement in symptomatology, including relief from the burning sensation, and the lesion from the tongue completely disappeared. This evidence-based case report highlights the potential beneficial role of homeopathy in the treatment of geographic tongue.
Prolidase Enzyme Activity as a Potential Biomarker for Blast-Induced Traumatic Brain Injury: A Study in a Rat Model
Authors: Gupta Rahul , Bhat Dhananjaya , Shukla Dhaval , Subramanian Sarada , Bansal Puru , Krishna Geethu , Jayan Mini , Devi Bhagavatula Indira 
Published: April 2024

AbstractBlast-induced traumatic brain injury (TBI) poses a significant threat to individuals exposed to explosive events. We hypothesized that blast-induced neurotrauma is an oxidative stress to brain and hence prolidase (PD) enzyme, which is an antioxidant is recommended; its serum levels are better markers of degree of injury in the acute phase for TBI in a rat blast injury model. Results revealed that PD enzyme activity in the hippocampus showed a slight increase across high, medium, and low blast pressures, but remained lower than the sham group. However, serum PD enzyme activity levels were significantly higher in the blast-exposed groups compared to the sham group. Tau protein levels were significantly elevated in the blast-exposed groups. Longitudinal analysis demonstrated a decline in hippocampal PD activity over time, while tau protein levels progressively increased, suggesting a shift from initial oxidative stress to neurodegeneration. These findings suggest that blast injury triggers oxidative stress and subsequent neurodegenerative processes. The correlation with tau protein levels further supports the involvement of oxidative stress in neurodegeneration. In conclusion, this study provides insights into the underlying pathophysiological mechanisms of blast-induced TBI and highlights the potential utility of PD enzyme activity as a diagnostic marker.