bluuebunny/crossref_metadata_2025_split huggingface dataset:
Glycolysis inhibition induces anti-tumor central memory CD8+T cell differentiation upon combination with microwave ablation therapy
Authors: Tang Xinyu , Mao Xinrui , Ling Peiwen , Yu Muxin , Pan Hua , Wang Jiaming , Liu Mingduo , Pan Hong , Qiu Wen , Che Nan , Zhang Kai , Bao Feifan , Peng Hongwei , Ding Qiang , Wang Shui , Zhou Wenbin 
Published: May 2024

AbstractMinimally invasive thermal therapy is a successful alternative treatment to surgery in solid tumors with high complete ablation rates, however, tumor recurrence remains a concern. Central memory CD8+ T cells (TCM) play important roles in protection from chronic infection and cancer. Here we find, by single-cell RNA analysis of human breast cancer samples, that although the memory phenotype of peripheral CD8+ T cells increases slightly after microwave ablation (MWA), the metabolism of peripheral CD8+ T cells remains unfavorable for memory phenotype. In mouse models, glycolysis inhibition by 2-deoxy-D-glucose (2DG) in combination with MWA results in long-term anti-tumor effect via enhancing differentiation of tumor-specific CD44hiCD62L+CD8+ TCM cells. Enhancement of CD8+ TCM cell differentiation determined by Stat-1, is dependent on the tumor-draining lymph nodes (TDLN) but takes place in peripheral blood, with metabolic remodeling of CD8+ T cells lasting the entire course of the the combination therapy. Importantly, in-vitro glycolysis inhibition in peripheral CD8+ T cells of patients with breast or liver tumors having been treated with MWA thrice leads to their differentiation into CD8+ TCM cells. Our work thus offers a potential strategy to avoid tumor recurrence following MWA therapy and lays down the proof-of-principle for future clinical trials.
Exploring high-quality microbial genomes by assembling short-reads with long-range connectivity
Authors: Zhang Zhenmiao , Xiao Jin , Wang Hongbo , Yang Chao , Huang Yufen , Yue Zhen , Chen Yang , Han Lijuan , Yin Kejing , Lyu Aiping , Fang Xiaodong , Zhang Lu 
Published: May 2024

AbstractAlthough long-read sequencing enables the generation of complete genomes for unculturable microbes, its high cost limits the widespread adoption of long-read sequencing in large-scale metagenomic studies. An alternative method is to assemble short-reads with long-range connectivity, which can be a cost-effective way to generate high-quality microbial genomes. Here, we develop Pangaea, a bioinformatic approach designed to enhance metagenome assembly using short-reads with long-range connectivity. Pangaea leverages connectivity derived from physical barcodes of linked-reads or virtual barcodes by aligning short-reads to long-reads. Pangaea utilizes a deep learning-based read binning algorithm to assemble co-barcoded reads exhibiting similar sequence contexts and abundances, thereby improving the assembly of high- and medium-abundance microbial genomes. Pangaea also leverages a multi-thresholding algorithm strategy to refine assembly for low-abundance microbes. We benchmark Pangaea on linked-reads and a combination of short- and long-reads from simulation data, mock communities and human gut metagenomes. Pangaea achieves significantly higher contig continuity as well as more near-complete metagenome-assembled genomes (NCMAGs) than the existing assemblers. Pangaea also generates three complete and circular NCMAGs on the human gut microbiomes.
In-situ observation of silk nanofibril assembly via graphene plasmonic infrared sensor
Authors: Wu Chenchen , Duan Yu , Yu Lintao , Hu Yao , Zhao Chenxi , Ji Chunwang , Guo Xiangdong , Zhang Shu , Dai Xiaokang , Ma Puyi , Wang Qian , Ling Shengjie , Yang Xiaoxia , Dai Qing 
Published: May 2024

AbstractSilk nanofibrils (SNFs), the fundamental building blocks of silk fibers, endow them with exceptional properties. However, the intricate mechanism governing SNF assembly, a process involving both protein conformational transitions and protein molecule conjunctions, remains elusive. This lack of understanding has hindered the development of artificial silk spinning techniques. In this study, we address this challenge by employing a graphene plasmonic infrared sensor in conjunction with multi-scale molecular dynamics (MD). This unique approach allows us to probe the secondary structure of nanoscale assembly intermediates (0.8–6.2 nm) and their morphological evolution. It also provides insights into the dynamics of silk fibroin (SF) over extended molecular timeframes. Our novel findings reveal that amorphous SFs undergo a conformational transition towards β-sheet-rich oligomers on graphene. These oligomers then connect to evolve into SNFs. These insights provide a comprehensive picture of SNF assembly, paving the way for advancements in biomimetic silk spinning.
ZDHHC20-mediated S-palmitoylation of YTHDF3 stabilizes MYC mRNA to promote pancreatic cancer progression
Authors: Zhang Huan , Sun Yan , Wang Zhaokai , Huang Xiaoju , Tang Lu , Jiang Ke , Jin Xin 
Published: May 2024

AbstractPost-translational modifications of proteins in malignant transformation and tumor maintenance of pancreatic ductal adenocarcinoma (PDAC) in the context of KRAS signaling remain poorly understood. Here, we use the KPC mouse model to examine the effect of palmitoylation on pancreatic cancer progression. ZDHHC20, upregulated by KRAS, is abnormally overexpressed and associated with poor prognosis in patients with pancreatic cancer. Dysregulation of ZDHHC20 promotes pancreatic cancer progression in a palmitoylation-dependent manner. ZDHHC20 inhibits the chaperone-mediated autophagic degradation of YTHDF3 through S-palmitoylation of Cys474, which can result in abnormal accumulation of the oncogenic product MYC and thereby promote the malignant phenotypes of cancer cells. Further, we design a biologically active YTHDF3-derived peptide to competitively inhibit YTHDF3 palmitoylation mediated by ZDHHC20, which in turn downregulates MYC expression and inhibits the progression of KRAS mutant pancreatic cancer. Thus, these findings highlight the therapeutic potential of targeting the ZDHHC20–YTHDF3–MYC signaling axis in pancreatic cancer.
Structural conversion of the spidroin C-terminal domain during assembly of spider silk fibers
Authors: De Oliveira Danilo Hirabae , Gowda Vasantha , Sparrman Tobias , Gustafsson Linnea , Sanches Pires Rodrigo , Riekel Christian , Barth Andreas , Lendel Christofer , Hedhammar My 
Published: May 2024

AbstractThe major ampullate Spidroin 1 (MaSp1) is the main protein of the dragline spider silk. The C-terminal (CT) domain of MaSp1 is crucial for the self-assembly into fibers but the details of how it contributes to the fiber formation remain unsolved. Here we exploit the fact that the CT domain can form silk-like fibers by itself to gain knowledge about this transition. Structural investigations of fibers from recombinantly produced CT domain from E. australis MaSp1 reveal an α-helix to β-sheet transition upon fiber formation and highlight the helix No4 segment as most likely to initiate the structural conversion. This prediction is corroborated by the finding that a peptide corresponding to helix No4 has the ability of pH-induced conversion into β-sheets and self-assembly into nanofibrils. Our results provide structural information about the CT domain in fiber form and clues about its role in triggering the structural conversion of spidroins during fiber assembly.
A labelled dataset to classify direct deforestation drivers from Earth Observation imagery in Cameroon
Authors: Debus Amandine , Beauchamp Emilie , Acworth James , Ewolo Achille , Kamga Justin , Verhegghen Astrid , Zébazé Christiane , Lines Emily R. 
Published: May 2024

AbstractUnderstanding direct deforestation drivers at a fine spatial and temporal scale is needed to design appropriate measures for forest management and monitoring. To achieve this, reference datasets with which to design Artificial Intelligence (AI) approaches to classify direct deforestation drivers within areas experiencing forest loss in a detailed, comprehensive and locally-adapted way are needed. This is the case for Cameroon, in the Congo Basin, which has known increasing deforestation rates in recent years. Here, we created an Earth Observation dataset with associated labels to classify detailed direct deforestation drivers in Cameroon, which includes satellite imagery (Landsat and PlanetScope) and auxiliary data on infrastructure and biophysical properties. The dataset provides the following fifteen labels: oil palm, timber, fruit, rubber and other-large scale plantations; grassland/shrubland; small-scale oil palm or maize plantations and other small-scale agriculture; mining; selective logging; infrastructure; wildfires; hunting; and other.
No effect of tattoos on local sweat concentrations of select cytokines, cortisol, glucose, blood urea nitrogen, or lactate during exercise
Authors: Merritt James R. , Ozga Michal , De Chavez Peter John D. , Boolani Ali , Baker Lindsay B. 
Published: May 2024

AbstractDue to growing interest in the investigation of exercise induced sweat biomarkers to assess an individual’s health and the increasing prevalence of tattoos in the world’s population, investigators sought to determine whether local sweat concentrations and excretion rates of epidermal growth factor (EGF), interleukin (IL) -1α, IL-6, IL-8, cortisol, glucose, blood urea nitrogen (BUN), and lactate differ between tattooed and contralateral non-tattooed skin during exercise. Sixteen recreational exercisers [female (50%)] (age = 25–48 years) with ≥ 1 unilateral permanent tattoo [median tattoo age = 6 years, IQR = 5] on the arm/torso completed an outdoor group fitness session. There were no significant differences between tattooed and non-tattooed skin for sweat EGF, IL-1α, IL-8, cortisol, glucose, BUN, or lactate concentrations. There were no significant differences between tattooed and non-tattooed skin for sweat EGF, IL-1α, IL-8, cortisol, glucose, BUN, or lactate excretion rate. Findings suggest that permanent tattoos older than 1 year may not impact local sweat EGF, IL-1α, IL-8, cortisol, glucose, BUN, and lactate concentrations or excretion rates during exercise.Clinical trial identifier NCT04920266 was registered on June 9, 2021.
Hindered phenolic antioxidant grafting on tailoring the DC electrical characteristics of polypropylene cable insulation
Authors: Du Boxue , Sun Guoning , Wang Heyu , Li Zhonglei 
Published: May 2024

AbstractThe authors focus on the impact of melt‐free radical grafting with hindered phenolic antioxidants (AO3052) on the electrical properties of polypropylene (PP) for DC cable insulation. The DC conductivity, space charge distribution and breakdown characteristic tests of grafting‐modified PP are performed by comparing unmodified PP. The results demonstrate that the grafting of antioxidants can effectively suppress space charge injection, owing to the deeper trap sites at the grafting molecule. The breakdown strength of the grafted PP is significantly enhanced from 30°C to 90°C and especially achieves a 5.3%–6.7% increase after the same DC‐prestressed time at 90°C. The surface electrostatic potential and molecular orbitals of the grafted PP are calculated. Simulation shows that the antioxidant introduces multi‐level local state traps that can effectively trap the injected space charge, thus decreasing the destruction of molecular chains by electrons and increasing the breakdown strength level. In conclusion, antioxidant grafting modification can improve the breakdown characteristics with or without DC prestress, and thus it appears to be promising in the application of PP‐insulated cables.
Editorial
Authors: Apel Marcel 
Published: May 2024

Liebe Leserinnen und Leser,es ist wieder an der Zeit für eine neue Ausgabe von Radiopraxis, und ich freue mich,
Sie durch die interessanten Inhalte des Juni-Hefts zu führen. Unsere Autorinnen und
Autoren haben sich mit aktuellen Themen auseinandergesetzt, die die Radiologie und
Radiologietechnologie prägen.
Construct validity and test-retest reliability of the Modified Agility T-Test in healthy adults
Authors: Zürcher John , Liechti Seraina , Degenfellner Jürgen , Pfeiffer Fabian , Bauer Christoph 
Published: May 2024

Abstract
Background After a lower limb injury, adequate agility is decisive for safe direction changes and reduces the risk of re-injury upon return to sports. Experts recommend that patients should pass standardized return to sports testing which involves agility tests such as the Modified Agility T-Test.
Aim Since the quality criteria of the Modified Agility T-Test have not been conclusively clarified, the objective of this study was to evaluate the construct validity and test-retest reliability of the Modified Agility T-Test.
Methods The study was conducted as a single-center study in a cross-sectional design comparing the performance of the Modified Agility T-Test with the Illinois Agility Test to evaluate the construct validity of the Modified Agility T-Test. The construct validity was calculated with the Pearson’s correlation coefficient. Absolute and relative reliability were calculated based on the test-retest results. Each participant performed two counting trials of both agility tests. To determine the absolute test-retest reliability, the standard error of measurement, 95 % limits of agreement and the smallest detectable change were calculated. To determine the relative test-retest reliability, the intraclass correlation coefficient 2.1 was calculated.
Results A total of 30 participants were recruited, with equal sex distribution and a mean age of 25.7 years. Our results showed a high construct validity of the Modified Agility T-Test (r = 0.89). The absolute test-retest reliability of the Modified Agility T-Test was 0.18 (-0.38–0.62) seconds, whereas the smallest detectable change was calculated to be 0.71 seconds. The relative test-retest reliability amounted to 0.84 (ICC 2.1).
Conclusions Our findings support the construct validity and test-retest reliability of the Modified Agility T-Test as an agility test. Thus, it could be used as an alternative to the Illinois Agility Test, particularly in sports which require sideways or backwards movements and for sports with short or rapid displacements.
Unemployment, hustling, and waithood: exploring Zimbabwean urban male youth’s utilisation of ICT in soccer betting
Authors: Magidi Martin , Gwekwerere Tavengwa 
Published: May 2024

AbstractThis article explores how urban male youth in Zimbabwe have created economies of hustle and waithood through soccer betting to ensure alternative means of survival in a country facing rapid urban growth and endless economic challenges. Data were collected using a combination of ethnographic methods, including participant and non-participant observations, betting experimentation, and informal conversations. The findings show that urban male youth in Zimbabwean cities use a variety of skills in soccer betting to increase their chances of winning and earning a living through informal economic activities while waiting for employment opportunities. The findings also demonstrate the intellectual genius and streetwise resourcefulness of young people, as they incorporate research, digital, and networking competencies to decide and place their bets. The article concludes that soccer betting is not only part of Zimbabwe’s economies of hustle, leisure, and waithood but also central in influencing the adoption of ICT products and digital skills among male youths in Zimbabwe.
Design and Application of an S-band Fast Radio Bursts Search Pipeline for the Nanshan 26 m Radio Telescope
Authors: Liu Yan-Ling , Chen Mao-Zheng , Li Jian , Yuan Jian-Ping , Yuen Rai , Liu Zhi-Yong , Yan Hao , Du Wen-Long , Zhai Nan-Nan 
Published: May 2024

Abstract
Fast radio bursts (FRBs) are among the most studied radio transients in astrophysics, but their origin and radiation mechanism are still unknown. It is a challenge to search for FRB events in a huge amount of observational data with high speed and high accuracy. With the rapid advancement of the FRB research process, FRB searching has changed from archive data mining to either long-term monitoring of the repeating FRBs or all-sky surveys with specialized equipments. Therefore, establishing a highly efficient and high quality FRB search pipeline is the primary task in FRB research. Deep learning techniques provide new ideas for FRB search processing. We have detected radio bursts from FRB 20201124A in the L-band observational data of the Nanshan 26 m radio telescope (NSRT-26m) using the constructed deep learning based search pipeline named dispersed dynamic spectra search (DDSS). Afterwards, we further retrained the deep learning model and applied the DDSS framework to S-band observations. In this paper, we present the FRB observation system and search pipeline using the S-band receiver. We carried out search experiments, and successfully detected the radio bursts from the magnetar SGR J1935+2145 and FRB 20220912A. The experimental results show that the search pipeline can complete the search efficiently and output the search results with high accuracy.
Lubricant savings in sheet metal forming through thermally oxidized wear protection layers
Authors: Müller P. , Holländer U. , Lendiel I. , Maier H.-J. , Hübner S. , Wehmeyer J. , Fünfkirchler T. , Behrens B.-A. 
Published: May 2024

Abstract
Increasing demands on quality, variety of shapes and complexity of deep-drawn components with simultaneous price stability make the development of innovative solutions in the field of deep-drawing indispensable. For ecological and economic reasons, there is a great interest in reducing the amount of lubricant required in forming processes to a minimum. In this work, tool coatings for forming tools are to be investigated and further developed in order to use their friction-reducing properties on the tribo-system and thus enable a reduction in the use of lubricants. For this purpose, in this research strip drawing tests are carried out to investigate the friction-influencing properties of different coatings and their potential for lubricant saving. Therefore, cylindrical forming heads are provided with different coating systems. Strips of aluminum sheet are drawn around the forming heads at an angle of 90°. The amount of lubricant applied is varied. Friction values are recorded and analyzed for each system. Wear mechanisms are identified, examined and evaluated macroscopically and microscopically. For this purpose, energy dispersive X-ray spectroscope (EDX) and scanning electron microscope (SEM) examinations are carried out. A statement on the potential of the investigated coatings for lubricant saving is derived from the results.
Influence of local heat treatment of rivets on the joint formation of a versatile joining process
Authors: Kappe F. , Bobbert M. , Meschut G. 
Published: May 2024

Abstract
An efficient lightweight construction method is the combination of different materials in order to adapt the structure to the applied load. To join these multi-material structures mechanical joining technologies are applied. However, the rigid tooling systems cannot be adjusted to changing boundary conditions which is why new, versatile joining technologies are required. In the versatile self-piercing riveting (V-SPR) process presented in [1] different material combination are joined by using a multi-range capable rivet. The rivet head is formed onto the respective thickness of the joint by an outer punch. In order to punch thru the upper sheet a great rivet hardness is required whereas a lower hardness is required for the subsequent forming of the rivet head. To achieve a combination of these requirements, this study investigates a local heat treatment of the rivet. The aim is to determine the feasibility of such a heat treatment as well as to investigate the influence on the joint formation.
Ductile fracture of tubular specimens under non-proportional loading condition
Authors: Baral Madhav , Korkolis Yannis P. 
Published: May 2024

Abstract
The ductile fracture behaviours of two tubular specimens, a AA6260-T4 macrotube (60 mm diameter and 2 mm thickness), and a SS-304L microtube (2.38 mm diameter and 0.15 mm thickness) under non-proportional loading condition are studied using a combined experimental-numerical approach. The experiments are conducted by loading the tubes under axial force and internal pressure along various non-proportional (i.e., corner) paths until failure by controlling the force/pressure ratio. The plastic behaviours of the tubes are characterized using the non-quadratic anisotropic yield criteria Yld2000-2D and Yld2004-3D. The material models are employed in the finite element (FE) simulation of the tube experiments using Abaqus/Standard (implicit). The FE models, which include thickness imperfection to capture the failure modes observed in the experiments, are then used to probe the fracture parameters inside the tube wall where fracture is likely to initiate. It is observed that the fracture strains from the non-proportional loading results are noticeably different when introduced to the proportional fracture strains, revealing the path-dependence fracture behaviour in tubular specimens.
Study on GISSMO fracture model of hot-stamped Tailored-Tempering-Properties parts
Authors: Qiu B Y , Wang Y L , Zhu B , Liu W , Zhang Y S , Ren X Q , Ren X P 
Published: May 2024

Abstract
To accommodate the varying mechanical demands in different areas of vehicle components, the Tailored-Tempering-Properties technology of hot-stamped parts of high-strength steel came into being. This research crafted a segregated hot and cold partition die system to fabricate U-shaped parts from 22MnB5 with Tailored-Tempering-Properties. The sides and bottom surfaces of the hard and soft zones of the U-shaped parts were sampled, and uniaxial tensile experiments were carried out to obtain the stress-strain curve, tensile strength, and elongation. In addition, notch, shear, and tensile shear sampling were carried out in the soft area at the bottom flange of the U-shaped part, and the force-displacement curve and fracture strain under different stress conditions were determined by tensile test. The GISSMO fracture model was selected to simulate the failure fracture of the material, and the parameters were obtained by Hypermesh pretreatment, LS-DYNA solution calculation, and LS-OPT optimization. Finally, the GISSMO fracture model suit for the soft zone of 22MnB5 U-shaped parts was obtained to characterize and predict its fracture behavior, which is an essential basis for guiding the design and optimization of parts.
Holistic springback compensation procedure
Authors: Zimmermann P , Birkert A , Saup P , Marner F , Häussermann M 
Published: May 2024

Abstract
The elastic springback during the manufacturing process of stamped car body components causes dimensional deviations. To compensate these deviations, the common approach is to modify the tool surfaces in the opposite direction of the deviations – this is called springback compensation. In the procedure of springback compensation various issues must be solved. To achieve the main goal of a dimensionally accurate part, it must be ensured that, on the one hand the distance normal to the sheet surface between the springback part and the target geometry is within a specified dimensional tolerance. On the other hand, it must be ensured that the surface areas and characteristic lengths of the springback part and that of the target geometry match as closely as possible. In the past, approaches/methods, such as the physical compensation method and the physical scaling approach, have been presented which can successfully counteract these problems. Furthermore, in a multi-stage process in subsequent operations a stable part position must be achieved and unwanted plastic deformations must be avoided during blankholder closing. Therefore, different compensation strategies have been presented, which can fulfil these requirements. However, in the publication of these methods, the problems were always considered individually. This paper shows how all the named requirements can be achieved by combining the individual methods in springback compensation.