bluuebunny/crossref_metadata_2025_split huggingface dataset:
Sex- and species-specific contribution of CD99 to T cell costimulation during multiple sclerosis
Authors: Winschel Ingo , Willing Anne , Engler Jan Broder , Walkenhorst Mark , Meurs Nina , Binkle-Ladisch Lars , Woo Marcel S. , Pfeffer Lena Kristina , Sonner Jana K. , Borgmeyer Uwe , Hagen Sven Hendrik , Grünhagel Benjamin , Claussen Janna M. , Altfeld Marcus , Friese Manuel A. 
Published: May 2024

Abstract
Background
Differences in immune responses between women and men are leading to a strong sex bias in the incidence of autoimmune diseases that predominantly affect women, such as multiple sclerosis (MS). MS manifests in more than twice as many women, making sex one of the most important risk factor. However, it is incompletely understood which genes contribute to sex differences in autoimmune incidence. To address that, we conducted a gene expression analysis in female and male human spleen and identified the transmembrane protein CD99 as one of the most significantly differentially expressed genes with marked increase in men. CD99 has been reported to participate in immune cell transmigration and T cell regulation, but sex-specific implications have not been comprehensively investigated.

Methods
In this study, we conducted a gene expression analysis in female and male human spleen using the Genotype-Tissue Expression (GTEx) project dataset to identify differentially expressed genes between women and men. After successful validation on protein level of human immune cell subsets, we assessed hormonal regulation of CD99 as well as its implication on T cell regulation in primary human T cells and Jurkat T cells. In addition, we performed in vivo assays in wildtype mice and in Cd99-deficient mice to further analyze functional consequences of differential CD99 expression.

Results
Here, we found higher CD99 gene expression in male human spleens compared to females and confirmed this expression difference on protein level on the surface of T cells and pDCs. Androgens are likely dispensable as the cause shown by in vitro assays and ex vivo analysis of trans men samples. In cerebrospinal fluid, CD99 was higher on T cells compared to blood. Of note, male MS patients had lower CD99 levels on CD4+ T cells in the CSF, unlike controls. By contrast, both sexes had similar CD99 expression in mice and Cd99-deficient mice showed equal susceptibility to experimental autoimmune encephalomyelitis compared to wildtypes. Functionally, CD99 increased upon human T cell activation and inhibited T cell proliferation after blockade. Accordingly, CD99-deficient Jurkat T cells showed decreased cell proliferation and cluster formation, rescued by CD99 reintroduction.

Conclusions
Our results demonstrate that CD99 is sex-specifically regulated in healthy individuals and MS patients and that it is involved in T cell costimulation in humans but not in mice. CD99 could potentially contribute to MS incidence and susceptibility in a sex-specific manner.
Cooperative Localization under Ionospheric Scintillation Events
Authors: Silva Paulo R. P. , Bruno Marcelo G. S. , Moraes Alison O. 
Published: May 2024

AbstractIonospheric scintillation causes major impairments to Global Navigation Satellite System (GNSS) in low-latitude regions. In severe scenarios, this event can lead to complete loss of lock, thus making GNSS measurements unusable for navigation. In this paper, we derive a cooperative localization algorithm where a set of partially connected aircraft exchange messages with neighboring nodes on the network to improve their own position estimates. We consider the scintillation events as abrupt changes in the measurement variance, which are modeled by a discrete-valued Markov process at the nodes which have access to GNSS measurements. Simulation results show that Markovian modeling and cooperation via factor graph message passing reduce the average 3D root mean square localization error and yield an average vertical position error that meets civil aviation standards for approach and landing.
Upper bounds for the spectral radius of matrices having the Perron–Frobenius property
Authors: Li Kaimin , Li Chaoqian 
Published: May 2024

AbstractA new upper bound for the spectral radius of matrices having the Perron–Frobenius property is given by considering the position of positive entries. Some examples involving the largest zero of polynomials and the spectral radius of the iterative matrix for the Perron–Frobenius splitting are given to show the superiority of the theoretical result.
A proximal gradient method with double inertial steps for minimization problems involving demicontractive mappings
Authors: Mouktonglang Thanasak , Chaiwino Wipawinee , Suparatulatorn Raweerote 
Published: May 2024

AbstractIn this article, we present a novel proximal gradient method based on double inertial steps for solving fixed points of demicontractive mapping and minimization problems. We also establish a weak convergence theorem by applying this method. Additionally, we provide a numerical example related to a signal recovery problem.
MTG_CD: Multi-scale learnable transformation graph for fault classification and diagnosis in microservices
Authors: Chen Juan , Zhang Rui , Chen Peng , Ren Jianhua , Wu Zongling , Wang Yang , Li Xi , Xiong Ling 
Published: May 2024

AbstractThe rapid advancement of microservice architecture in the cloud has led to the necessity of effectively detecting, classifying, and diagnosing run failures in microservice applications. Due to the high dynamics of cloud environments and the complex dependencies between microservices, it is challenging to achieve robust real-time system fault identification. This paper proposes an interpretable fault diagnosis framework tailored for microservice architecture, namely Multi-scale Learnable Transformation Graph for Fault Classification and Diagnosis(MTG_CD). Firstly, we employ multi-scale neural transformation and graph structure adjacency matrix learning to enhance data diversity while extracting temporal-structural features from system monitoring metrics Secondly, a graph convolutional network (GCN) is utilized to fuse the extracted temporal-structural features in a multi-feature modeling approach, which helps to improve the accuracy of anomaly detection. To identify the root cause of system faults, we finally conduct a coarse-grained level diagnosis and exploration after obtaining the results of classifying the fault data. We evaluate the performance of MTG_CD on the microservice benchmark SockShop, demonstrating its superiority over several baseline methods in detecting CPU usage overhead, memory leak, and network delay faults. The average macro F1 score improves by 14.05%.
Evaluation of antioxidant and anti-inflammatory activity and identification of bioactive compound from the marine diatom, Odontella aurita extract
Authors: Cuong Do Manh , Yang Sun Hee , Kim Ji Soo , Moon Jeong Yong , Choi Jongkeun , Go Gyung Min , Cho Somi Kim 
Published: May 2024

AbstractIncreased production of reactive oxygen species (ROS) leads to oxidative stress, with its damaging effect extending to the mitochondria and plasma membrane. Further, prolonged inflammation can result in chronic disease development. The marine microdiatom Odontella aurita is recognized for its potential in food and pharmaceutical development. Moreover, it contains antioxidant and anti-inflammatory properties. However, studies regarding the efficacy of their varying extract forms and their underlying mechanisms remain scarce. Therefore, this study aims to explore the antioxidant and anti-inflammatory effects of Odontella aurita extracts obtained using various extraction methods (hot water, 70% ethanol, and chloroform:methanol (CM)). Among the three Odontella aurita extracts, the CM extract demonstrated superior efficacy in protecting RAW 264.7 cells from H2O2-induced cytotoxicity. It significantly lowered the levels of ROS and enhanced the expression of superoxide dismutase and glutathione peroxidase. Furthermore, the CM extract outperformed other extracts in inhibiting LPS-induced nitric oxide production, reducing mRNA levels in nitric oxide synthase, cyclooxygenase, and the proinflammatory cytokines interleukin IL-1β, IL-6, TNFα. Additionally, CM extract effectively suppressed the activation of NF-κB/IκBα and JAK2-STAT3 in LPS-induced RAW 264.7 cells. HPLC–UV analysis revealed a remarkable 33-fold higher fucoxanthin content in CM compared to the ethanol extract. GC–MS analysis identified elevated levels of cholest-5-en-3-ol, phytol, eicosapentaenoic acid methyl ester, methyl palmitate, palmitoleic acid methyl ester, and neophytadiene in the CM extract. These findings suggest that Odontella aurita CM extract is a promising antioxidant candidate for preventing or treating inflammatory diseases, consequently emphasizing its potential for further development.
Graphical Abstract
The clinical significance of emotional urgency in bipolar disorder: a scoping review
Authors: Teh Wen Lin , Si Sheng Yeow , Liu Jianlin , Subramaniam Mythily , Ho Roger 
Published: May 2024

Abstract
Background
Emotional urgency, defined as a trait concept of emotion-based impulsivity, is at least moderately associated with general psychopathology. However, its clinical significance and associations with clinically relevant features of bipolar disorder remain unclear. This scoping review aims address this gap by determining the extent of evidence in this niche scope of study.

Methods
Evidence of between-group differences of positive and negative urgency, its associations with mood severity, and all peripheral associations related to illness and psychosocial outcomes were synthesized based on PRISMA checklists and guidelines for scoping reviews (PRISMA-ScR).

Design
Electronic databases were searched for articles published between January 2001 and January 2024. A total of 1013 entries were gathered, and a total of 10 articles were included in the final selection after the removal of duplicates and ineligible articles.

Results
Differences in urgency scores between bipolar disorder and healthy controls were large (Cohen’s d ranged from 1.77 to 2.20). Negative urgency was at least moderately associated with overall trauma, emotional abuse, neglect, suicide ideation, neuroticism, and irritable/cyclothymic temperament, whereas positive urgency was at least moderately associated with various aspects of aggression and quality of life. Positive but not negative urgency was associated with quality of life in bipolar disorder.

Conclusion
Large between-group differences found for emotional urgency in bipolar disorder imply large clinical significance. Emotional urgency was associated with worse clinical features and outcomes. Given the high clinical heterogeneity of the disorder, emotional urgency may be an important phenotype indicative of greater disorder severity.
Assessment of immediate and five-year earthquake impacts on river systems in sabah, Malaysia using multi-temporal satellite imageries
Authors: Chai Lee Ting , Nainar Anand , Roslee Rodeano , Wong Wilson Vun Chiong , Phua Mui-How 
Published: May 2024

Abstract
Background
Earthquake is one of the most destructive natural disasters, which cause immediate and long-term changes to the river systems. This research aimed to examine the immediate and five-year impacts of the 2015 Ranau Earthquake (6.0 Mw) on river systems in Malaysian state of Sabah, a region of low earthquake hazard.

Methods
We used object-based classification on Landsat 8 (2014 and 2015) and Sentinel-2A (2020) satellite imageries to derive land cover time series for investigating the impacts on the riparian areas.

Results
The earthquake removed vegetation in the riparian zones of four rivers, the highest being the Penataran River (69.21 ha). During the immediate impact period (2014–2015), river bar formation occurred in all rivers, with the largest increase occurring in the Kadamaian River (56.97 ha), followed by the Panataran River (54.36 ha), which had no river bar before the earthquake. The river bar of the Kadamaian River continued to increase, whereas the river bar of the Panataran River decreased five years after the earthquake. Land cover transition analysis revealed that 78.39 ha of vegetation, barren land, and river water areas changed to river bars in the Kadamaian riparian area during the immediate impact period. Except for 26.87% of river bars in the Kadamaian riparian area in 2015, most river bars transitioned to other land cover types five years later. During the period of immediate impact, 22.05 ha of vegetation and 10.71 ha of river water were transformed into river bars along the Penataran River. Five years later, except for 16.2 ha, all river bar areas had transitioned to other cover types. Additionally, 17.7 ha of new river bars were formed. This study provides crucial data on post-earthquake land cover changes, particularly river bar formation and changes, for assessing the earthquake impacts on the river systems and supporting impact mitigation.
Research on re-searching: interrupted foraging is not disrupted foraging
Authors: Hong Injae , Wolfe Jeremy M. 
Published: May 2024

AbstractIn classic visual search, observers typically search for the presence of a target in a scene or display. In foraging tasks, there may be multiple targets in the same display (or “patch”). Observers typically search for and collect these target items in one patch until they decide to leave that patch and move to the next one. This is a highly rule-governed behavior. The current study investigated whether these rules are disrupted when the foraging is interrupted in various manners. In Experiment 1, the foraging was briefly interrupted and then resumed in the same patch. In Experiments 2 and 3, the foraging in each patch either ended voluntarily or compulsorily after a fixed amount of time. In these cases, foraging resumed in a patch only after all patches were visited. Overall, the rules of foraging remained largely intact, though Experiment 2 shows that foraging rules can be overridden by the demand characteristics of the task. The results show that participants tended to perform approximately consistently despite interruptions. The results suggest that foraging behavior in a relatively simple foraging environment is resilient and not easily disrupted by interruption.
Estimating the volume of penumbra in rodents using DTI and stack-based ensemble machine learning framework
Authors: Kuo Duen-Pang , Chen Yung-Chieh , Li Yi-Tien , Cheng Sho-Jen , Hsieh Kevin Li-Chun , Kuo Po-Chih , Ou Chen-Yin , Chen Cheng-Yu 
Published: May 2024

Abstract
Background
This study investigates the potential of diffusion tensor imaging (DTI) in identifying penumbral volume (PV) compared to the standard gadolinium-required perfusion–diffusion mismatch (PDM), utilizing a stack-based ensemble machine learning (ML) approach with enhanced explainability.

Methods
Sixteen male rats were subjected to middle cerebral artery occlusion. The penumbra was identified using PDM at 30 and 90 min after occlusion. We used 11 DTI-derived metrics and 14 distance-based features to train five voxel-wise ML models. The model predictions were integrated using stack-based ensemble techniques. ML-estimated and PDM-defined PVs were compared to evaluate model performance through volume similarity assessment, the Pearson correlation analysis, and Bland–Altman analysis. Feature importance was determined for explainability.

Results
In the test rats, the ML-estimated median PV was 106.4 mL (interquartile range 44.6–157.3 mL), whereas the PDM-defined median PV was 102.0 mL (52.1–144.9 mL). These PVs had a volume similarity of 0.88 (0.79–0.96), a Pearson correlation coefficient of 0.93 (p < 0.001), and a Bland–Altman bias of 2.5 mL (2.4% of the mean PDM-defined PV), with 95% limits of agreement ranging from -44.9 to 49.9 mL. Among the features used for PV prediction, the mean diffusivity was the most important feature.

Conclusions
Our study confirmed that PV can be estimated using DTI metrics with a stack-based ensemble ML approach, yielding results comparable to the volume defined by the standard PDM. The model explainability enhanced its clinical relevance. Human studies are warranted to validate our findings.

Relevance statement
The proposed DTI-based ML model can estimate PV without the need for contrast agent administration, offering a valuable option for patients with kidney dysfunction. It also can serve as an alternative if perfusion map interpretation fails in the clinical setting.

Key points
• Penumbral volume can be estimated by DTI combined with stack-based ensemble ML.
• Mean diffusivity was the most important feature used for predicting penumbral volume.
• The proposed approach can be beneficial for patients with kidney dysfunction.

Graphical Abstract
Benefits and advances of Cone Beam CT use in prostatic artery embolization: review of the literature and pictorial essay
Authors: Rostambeigi Nassir , Crawford Daniel , Golzarian Jafar 
Published: May 2024

AbstractProstatic artery embolization (PAE) has proven to be an efficacious treatment for urinary symptoms of benign prostatic hyperplasia. PAE is performed in a complex and challenging anatomical field which may pose difficulties from procedural standpoint. Cone beam computed tomography (CBCT) has been proposed as an invaluable tool during the PAE procedure. A review of different techniques and advancements, as well as demonstration of CBCT benefits via a pictorial overview of the salient examples is lacking. The techniques of CBCT are discussed herein and the virtual injection technology as an advancement in CBCT is discussed. To show the merits of CBCT in PAE, a pictorial overview of various clinical scenarios is presented where CBCT can be crucial in decision making. These scenarios are aimed at showing different benefits including identification of the origin of the prostatic artery and avoiding non-target embolization. Other benefits may include ensuring complete embolization of entire prostate gland as angiographic appearance alone can be inconclusive if it mimics a severely thickened bladder wall or ensuring adequate embolization of the median lobe to provide relief from “ball-valve” effect. Further examples include verification of embolization of the entire prostate when rare variants or multiple (> 2) arterial feeders are present.
Unintentionally intentional: unintended effects of spinal stimulation as a platform for multi-modal neurorehabilitation after spinal cord injury
Authors: Moreno Romero Gerson N. , Twyman Avery R. , Bandres Maria F. , McPherson Jacob Graves 
Published: May 2024

AbstractElectrical stimulation of spinal neurons has emerged as a valuable tool to enhance rehabilitation after spinal cord injury. In separate parameterizations, it has shown promise for improving voluntary movement, reducing symptoms of autonomic dysreflexia, improving functions mediated by muscles of the pelvic floor (e.g., bowel, bladder, and sexual function), reducing spasms and spasticity, and decreasing neuropathic pain, among others. This diverse set of actions is related both to the density of sensorimotor neural networks in the spinal cord and to the intrinsic ability of electrical stimulation to modulate neural transmission in multiple spinal networks simultaneously. It also suggests that certain spinal stimulation parameterizations may be capable of providing multi-modal therapeutic benefits, which would directly address the complex, multi-faceted rehabilitation goals of people living with spinal cord injury. This review is intended to identify and characterize reports of spinal stimulation-based therapies specifically designed to provide multi-modal benefits and those that report relevant unintended effects of spinal stimulation paradigms parameterized to enhance a single consequence of spinal cord injury.
Sonographic evaluation of peripheral nerve involvement in leprosy with electrophysiologic correlation: a cross-sectional study in sub-Himalayan region
Authors: Aggarwal Neeti , Thakur Pooja , Kapila Sumala , Thakur Shruti , Jhobta Anupam , Makhaik Sushma , Thakur Charu Smita 
Published: May 2024

Abstract
Background
Leprosy is an age-old chronic infectious disease with the majority of annual new case detections from South-East Asia. The disease manifestations coupled with the stigma attached to it often creates grave socioeconomic problems. Leprosy is curable and if detected and treated in the early stages can prevent disability. Ultrasonography provides information regarding location and degree of the nerve damage, nerve morphologic alterations, echo texture, fascicular pattern and vascularity. The aim of this study was to study the ultrasonographic features of neuropathy in leprosy with electrophysiologic correlation.

Results
A total of 34 histopathological proven cases of leprosy were included in this study, which was conducted for 1 year. High-resolution ultrasound (HRUS) of a total of 204 peripheral nerves in these 34 patients, including bilateral ulnar, median and common peroneal nerves, was performed. Cross-sectional areas, nerve diameters, nerve morphology and vascularity were noted and correlated with electrophysiologic study of these nerves. The results showed that all the patients having reduced motor or sensory function [decreased compound muscle action potential (CMAP), decreased compound nerve action potential (SNAP) and increased latency] in ulnar and common peroneal nerves were thickened on HRUS (100% in ulnar and common peroneal nerves) while 92% right median and 89% left median nerves with reduced motor or sensory function showed thickening on HRUS. Also, 5.8% ulnar nerves and 11.7% common peroneal nerves showed thickening on HRUS; however, sensory or motor conduction of these nerves was unaffected on nerve conduction study (NCS). So, a positive correlation was observed for nerve involvement as detected by ultrasonographic findings of nerve hypertrophy and the electrophysiologic study. The most common finding was focal or diffuse nerve thickening. Ulnar nerve was the most commonly thickened nerve in leprosy patients with the most common location of nerve thickening at medial epicondyle.

Conclusions
Ultrasound and electrophysiologic study of peripheral nerves in leprosy are complimentary to each other in diagnosing leprotic neuropathy.