bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Simple Synthesis of Functionalized 3‐Bromocoumarins by a One‐Pot Three‐Component Reaction
Authors: Audisio Davide , Messaoudi Samir , Brion Jean‐Daniel , Alami Mouâd 
Published: January 2010

AbstractFunctionalized 3‐bromocoumarins 2 have been prepared by a simple one‐pot bromination/Wittig/cyclization tandem process from methyl (triphenylphosphoranylidene)acetate, N‐bromosuccinimide and a series of 2‐hydroxybenzaldehydes. Owing to the commercial availability of salicylaldehyde derivatives, this approach offers such a diverse range of compounds that it fulfills the recent demand for the generation of large combinatorial chemical libraries based on the coumarin scaffold.
Synthesis of Functionalized Hydropentalenes by an Asymmetric Deprotonation/Alkylation Strategy
Authors: Lutz Vanessa , Baro Angelika , Fischer Peter , Laschat Sabine 
Published: January 2010

AbstractThe functionalization of differently substituted hydropentalenone derivatives 9, derived from the Weiss diketone (8) by enantioselective deprotonation in the presence of lithium (R,R)‐bis(1‐phenylethyl)amide/LiCl (11·LiCl) as the chiral base is described. In the first route the resulting enolate was treated directly with alkyl halides as electrophiles to give the target α‐alkylhydropentalenones 12, whereas in the second route the enolate was trapped as one of the triethylsilyl enol ethers 17, from which the enolate was regenerated by treatment with MeLi prior to alkylation with alkyl halides. The substituents on 9 seemed to influence which strategy is favored: for the OTBS‐substituted hydropentalenone 9a the direct deprotonation/alkylation is preferred, whereas for the acetal‐substituted hydropentalenone 9b the silyl enol ether route is more suitable. In all cases the α‐alkylated hydropentalenones 12 and 15 were isolated with good diastereoselectivities.
Synthesis and Spectroscopic Characterization of the First Symmetrically and Nonsymmetrically Substituted Fluorinated Emerald‐Green Trannulenes C60F15R3 Soluble in Polar Media and Water
Authors: Troshin Pavel A. , Khakina Ekaterina A. , Zhilenkov Alexander V. , Peregudov Alexander S. , Troshina Olesya A. , Kozlovskii Viacheslav I. , Polyakova Natalya V. , Lyubovskaya Rimma N. 
Published: January 2010

AbstractThe first water‐soluble C3v‐symmetrical trannulene derivatives of fluorofullerene C60F18 possessing six carboxylic or ammonium ion groups have been synthesized and spectrally characterized. The stability of emerald‐green aqueous solutions of these compounds was investigated. A novel synthetic route was developed for a step‐by‐step derivatization of C60F18 with different organic C–H acids that yielded nonsymmetrically substituted C60F15R2R′ trannulenes. The synthesized trannulenes were loaded with polar carboxylic groups that improved greatly the solubility of these compounds in aqueous media. We showed that four solubilizing COOH groups could be introduced onto the fullerene cage of C60F15R2R′ trannulenes with R substituents and then some additional organic functionality could be attached independently with R′. The R′ substituent might potentially comprise a ligand responsible for selective targeting of the whole trannulene molecule to some specific tissues or intracellular structures. The demonstrated loading of the fluorofullerene core with polar carboxylic or ammonium groups might be considered as an important step towards the design of sophisticated water‐soluble trannulene‐based assembles for biomedical applications.
Efficient Synthesis of Glycoporphyrins by Microwave‐Mediated “Click” Reactions
Authors: Locos Oliver B. , Heindl Claudia C. , Corral Ariadna , Senge Mathias O. , Scanlan Eoin M. 
Published: January 2010

AbstractThe CuI‐catalysed Huisgen cycloaddition “click” reaction has been applied to the synthesis of a range of triazole‐linked glycoporphyrins. The “click” reaction under microwave‐heating conditions has been shown to provide a general and robust methodology for the synthesis of mono‐, di‐, tri‐ and tetra‐modified glycoporphyrins. A sequential “double‐click” process was employed to access a new class of bis‐modified 5,10‐diglycoporphyrins displaying heterogeneous carbohydrates.
Boron‐Doped Diamond Electrode Coupled to Liquid Chromatography: Application to Simultaneous Determination of Benzodiazepines
Authors: Martins Isarita , de Souza Canaes Larissa , Doretto Keity Margareth , Rath Susanne 
Published: January 2010

AbstractThe applicability of boron‐doped diamond (BDD) as a working electrode in an amperometric cell, coupled to HPLC, was demonstrated, for determining benzodiazepines in pharmaceutical preparations. The separation of the benzodiazepines was achieved using a Waters XTerra RP18 column (250×4.6 mm, 5 μm) with a mobile phase sodium phosphate (pH 3.5; 0.10 mol L−1)‐acetonitrile (65 : 35, v/v) at flow of 1.2 mL min−1. The measurements were performed in a system 871 Advanced Bioscan (Metrohm) with a BDD (8000 ppm) adapted to the thin layer mode cell. Stainless steel and platinum wire were used as reference and auxiliary electrodes, respectively. The cell was operated in pulse mode, using −1.9 V as initial potential. The method presented linearity, repeatability and ruggedness and it represents a novel, alternative electroanalytical method for benzodiazepines determination.
Electrochemically Induced Uptake and Release of Iron by Recombinant Horse H‐Chain Ferritin Covalently Immobilized on 6‐Mercaptohexanoic Acid Modified Gold Electrodes
Authors: Scott Brandon L. , Zapien Donald C. 
Published: January 2010

AbstractFerritin composed entirely of the H‐chain subunit (rHF) was studied to determine whether electrochemical potential alone could induce rHF to take up or release iron. Horse rHF was expressed in E. coli, purified, and covalently immobilized on a self‐assembled monolayer of 6‐mercaptohexanoic acid on gold electrodes. Cyclic voltammetry was used to show that iron was released from rHF following electrochemical reduction, and iron uptake was induced by applying an oxidizing potential to rHF in the presence of ferrous ion.
A Comparison of Membrane Inlet Mass Spectrometry and Nitric Oxide (NO) Electrode Techniques to Detect NO in Aqueous Solution
Authors: Tu Chingkuang , Scafa Nikki , Zhang Xueji , Silverman David N. 
Published: January 2010

AbstractThe NO electrode and membrane inlet mass spectrometry (MIMS) have the advantage of being sensitive, direct, and real time detectors of NO in aqueous solution. They do not require reacting NO with labels or purging of NO with an inert gas. We show that the NO electrode and MIMS are comparable in sensitivity detecting NO concentrations to 0.5 nM in aqueous solution, and both give identical results in a biological measurement, the reactions of deoxyhemoglobin with nitrite.
Numerical modeling of sloshing motion in a tuned liquid damper outfitted with a submerged slat screen
Authors: Maravani Morteza , Hamed M. S. 
Published: January 2010

AbstractTuned liquid dampers (TLDs) are among the most economical and effective passive damping devices. They have been increasingly used to reduce dynamic response and protect structures from failure due to external dynamic excitations. Slat screens are one of the most effective devices used to increase the inherent damping of a TLD, and to reduce the non‐linearity of the free surface motion. A numerical algorithm has been developed to solve the complete non‐linear, moving boundary flow problem in a TLD outfitted with slat screens. The model has been developed to handle conditions leading from small to large interfacial deformations without imposing any linearization assumptions. The numerical algorithm is based on the finite‐difference method. The free surface has been determined using the volume‐of‐fluid method and the donor–acceptor algorithm. The effect of the slat screens has been modeled explicitly by using the partial‐cell treatment method. The present algorithm has been validated against experimental data. The results indicated that the present algorithm is capable of providing accurate details of the flow field inside the TLD and through the screens. These details are essential to improve our understanding of the important parameters governing the performance of a TLD, and hence, to enhance our ability to design better TLDs. Copyright © 2010 John Wiley & Sons, Ltd.
Development of a multi‐scale ocean model by using particle Laplacian method for anisotropic mass transfer
Authors: Jeong Se‐min , Sato Toru , Chen Baixin , Tabeta Shigeru 
Published: January 2010

AbstractThe direct injection of CO2 into the deep ocean is one of the feasible ways for the mitigation of the global warming, although there is a concern about its environmental impact near the injection point. To minimize its biological impact, it is necessary to make CO2 disperse as quickly as possible, and it is said that injection with a pipe towed by a moving ship is effective for this purpose. Because the injection ship moves over a spatial scale of O(102km), a mesoscale model is necessary to analyse the dispersion of CO2. At the same time, since it is important to investigate high CO2 concentration near the injection point, a small‐scale model is also required. Therefore, in this study, a numerical model was developed to analyse CO2 dispersion in the deep ocean by using a fixed mesoscale and a moving small‐scale grid systems, the latter of which is nested and moves in the former along the trajectory of the moving ship. To overcome the artificial diffusion of mass concentration at the interface of the two different grid systems and to keep its spatial accuracy almost the same as that in the small‐scale, a particle Laplacian method was adopted and newly modified for anisotropic diffusion in the ocean. Copyright © 2010 John Wiley & Sons, Ltd.
Executive deficits and regional brain metabolism in Alzheimer's disease
Authors: Woo Benjamin K. P. , Harwood Dylan G. , Melrose Rebecca J. , Mandelkern Mark A. , Campa Olivia M. , Walston Amy , Sultzer David L. 
Published: January 2010

AbstractObjectiveExecutive deficits are common in patients with Alzheimer's disease (AD), contribute prominently to clinical disability, and may be associated with frontal lobe pathology. This study examined regional brain hypometabolism associated with executive dysfunction in patients with AD.MethodsForty‐one patients with probable AD underwent [18F] fluorodeoxyglucose positron emission tomography (FDG‐PET) imaging at rest. Neuropsychological measures of executive control included the Conceptualization (Conc) and Initiation/Perseveration (I/P) subscales of the Mattis Dementia Rating Scale (DRS), the Wechsler Adult Intelligence Scale (WAIS) Similarities subtest, the Tower test, and the Ruff Figural Fluency test (Ruff). Voxel‐based analyses were conducted using statistical parametric mapping (SPM2) to measure the correlation between regional cerebral metabolism and executive measures. Correlations independent of global cognitive impairment were identified by including Mini‐Mental State Examination (MMSE) score as a covariate in the model.ResultsExecutive deficits, as measured by poor performances on the DRS I/P and Conc subscales, were associated with hypometabolism in the bilateral mid‐dorsolateral frontal region. Activity in posterior cortical regions also contributed uniquely to some aspects of executive functioning, as lower resting metabolism in parietal or temporal cortex was correlated with poor performance on four of the five executive measures. After controlling for global cognitive score, there were significant extra‐frontal correlations with hypometabolism in insula, occipital lobe, and temporal cortex.ConclusionsSome but not all executive deficits in AD are associated with neural activity in the dorsolateral frontal cortex. Activities in distributed neural systems that include parietal and temporal cortex also contribute to some executive abilities. The pathophysiology of executive dysfunction is complex and includes abnormalities not limited to a single region. Copyright © 2010 John Wiley & Sons, Ltd.
Highly selective synthesis of 1‐phenylpentane‐1, 4‐diones, and (E)‐5‐hydroxy‐5‐phenylpent‐3‐en‐2‐ones catalyzed by organophosphorus compounds
Authors: Zhang Qing , Qiao Yan‐Fei , Huang Min , Liang Hong‐Dong , Zhou Ru‐Jin 
Published: January 2010

Abstract1‐Phenylpentane‐1, 4‐diones, and (E)‐5‐hydroxy‐5‐phenylpent‐3‐en‐2‐ones were synthesized via organophosphine‐catalyzed addition reaction of but‐3‐en‐2‐one with aldehydes. The features of the present protocols include high selectivity, operational simplicity, atom economy, and mild reaction conditions without transition metals. © 2010 Wiley Periodicals, Inc. Heteroatom Chem 20:425–430, 2009; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/hc.20568
Tensin 2 modulates cell contractility in 3D collagen gels through the RhoGAP DLC1
Authors: Clark Katherine , Howe Jonathan D. , Pullar Christine E. , Green J. Angelo , Artym Vira V. , Yamada Kenneth M. , Critchley David R. 
Published: January 2010

AbstractCytoskeletal proteins of the tensin family couple integrins to the actin cytoskeleton. They are found in both focal adhesions and the fibrillar adhesions formed between cells and the fibronectin matrix. There are four tensin genes which encode three large (∼200 kDa) tensin isoforms (tensin 1, 2, 3) and one short isoform (cten). However, the subcellular localization and function of the individual isoforms is poorly understood. Using human foreskin fibroblasts (HFFs), and imaging on both fixed and live cells, we show that GFP‐tensin 2 is enriched in dynamic focal adhesions at the leading edge of the cell, whereas GFP‐tensin 3 translocates rearward, and is enriched in fibrillar adhesions. To investigate the possible role of tensins in cell‐matrix remodeling, we used siRNAs to knockdown each tensin isoform. We discovered that tensin 2 knockdown significantly reduced the ability of HFFs to contract 3D collagen gels, whilst no effect on fibronectin fibrillogenesis was observed. This inhibition of collagen gel contraction was associated with a substantial reduction in Rho activity, and it was reversed by depletion of DLC1, a RhoGAP that binds to tensin in focal adhesions. These findings suggest that focal adhesion‐localized tensin 2 negatively regulates DLC1 to permit Rho‐mediated actomyosin contraction and remodeling of collagen fibers. J. Cell. Biochem. 109: 808–817, 2010. © 2010 Wiley‐Liss, Inc.
Wnt signaling in hematopoiesis: Crucial factors for self‐renewal, proliferation, and cell fate decisions
Authors: Staal Frank J.T. , Luis Tiago C. 
Published: January 2010

AbstractA large number of studies from many different laboratories have implicated the Wnt signaling pathway in regulation of hematopoiesis. However, different inducible gain‐ and loss‐of‐function approaches yielded controversial and some times contradictory results. In this prospect we will review the current ideas on Wnt signaling in hematopoiesis and early lymphopoiesis. Reviewing this large body of knowledge let us to hypothesize that different levels of activation of the pathway, dosages of Wnt signaling required and the interference by other signals in the context of Wnt activation collectively explain these controversies. Besides differences in dosage, differences in biological function of Wnt proteins in various blood cell types also is a major factor to take into account. Our own work has shown that while in the thymus Wnt signaling provides cytokine‐like, proliferative stimuli to developing thymocytes, canonical Wnt signaling in HSC regulates cell fate decisions, in particular self‐renewal versus differentiation. J. Cell. Biochem. 109: 844–849, 2010. © 2010 Wiley‐Liss, Inc.
Capsaicin induces apoptosis through ubiquitin–proteasome system dysfunction
Authors: Maity Ranjan , Sharma Jaiprakash , Jana Nihar Ranjan 
Published: January 2010

AbstractCapsaicin is an active component of red pepper having an antiproliferative effect in a variety of cancer cells, which recent evidence suggests due to its ability to induce apoptosis. However, the molecular mechanisms through which capsaicin induces apoptosis are not well understood. Here we demonstrate that capsaicin‐induced apoptosis is mediated via the inhibition cellular proteasome function. Treatment of capsaicin to mouse neuro 2a cells results in the inhibition of proteasome activity in a dose‐ and time‐dependent manner that seems to correlate with its effect on cell death. The effect of capsaicin on cellular proteasome function is indirect and probably mediated via the generation of oxidative stress. Exposure of capsaicin also causes increased accumulation of ubiquitinated proteins as wells as various target substrates of proteasome like p53 and Bax and p27. Like many other classical proteasome inhibitors, capsaicin also triggers the intrinsic pathway of apoptosis involving mitochondria and induces neurite outgrowth. Our results strongly support for the use of capsaicin as an anticancer drug. J. Cell. Biochem. 109: 933–942, 2010. © 2010 Wiley‐Liss, Inc.
Spatial insulin signalling in isolated skeletal muscle preparations
Authors: Sogaard Peter , Szekeres Ferenc , Garcia‐Roves Pablo M. , Larsson Dennis , Chibalin Alexander V. , Zierath Juleen R. 
Published: January 2010

AbstractDuring in vitro incubation in the absence or presence of insulin, glycogen depletion occurs in the inner core of the muscle specimen, concomitant with increased staining of hypoxia‐induced‐factor‐1‐alpha and caspase‐3, markers of hypoxia and apoptosis, respectively. The aim of this study was to determine whether insulin is able to diffuse across the entire muscle specimen in sufficient amounts to activate signalling cascades to promote glucose uptake and glycogenesis within isolated mouse skeletal muscle. Phosphoprotein multiplex assay on lysates from muscle preparation was performed to detect phosphorylation of insulin‐receptor on Tyr1146, Akt on Ser473 and glycogen‐synthases‐kinase‐3 on Ser21/Ser9. To address the spatial resolution of insulin signalling, immunohistochemistry studies on cryosections were performed. Our results provide evidence to suggest that during the in vitro incubation, insulin sufficiently diffuses into the centre of tubular mouse muscles to promote phosphorylation of these signalling events. Interestingly, increased insulin signalling was observed in the core of the incubated muscle specimens, correlating with the location of oxidative fibres. In conclusion, insulin action was not restricted due to insufficient diffusion of the hormone during in vitro incubation in either extensor digitorum longus or soleus muscles from mouse under the specific experimental settings employed in this study. Hence, we suggest that the glycogen depleted core as earlier observed is not due to insufficient insulin action. J. Cell. Biochem. 109: 943–949, 2010. © 2010 Wiley‐Liss, Inc.
Hydrogen peroxide induces p16INK4a through an AUF1‐dependent manner
Authors: Guo Gai E , Ma Li Wei , Jiang Bin , Yi Jie , Tong Tan Jun , Wang Wen Gong 
Published: January 2010

AbstractElevation of p16INK4a has been described as an important mechanism for hydrogen peroxide (H2O2)‐induced replicative senescence. However, the mechanisms underlying remain unknown. In this study, we demonstrate an important role of RNA‐binding protein AUF1‐mediated mRNA turnover in H2O2‐induced p16INK4a expression. The induction of p16 by H2O2 was accompanied with declined cytoplasmic AUF1 level. Accordingly, exposure of cells to H2O2 remarkably reduced the binding of AUF1 to p16 3′UTR and increased the half‐life of an EGFP‐p16‐3′UTR chimeric transcript. In AUF1‐silenced cells, the effect of H2O2 on p16 induction was abolished. Furthermore, in cells co‐transfected with vectors expressing AUF1s, treatment with H2O2 failed to significantly reduce the expression of AUF1 and subsequently elevate the levels of p16. Moreover, HeLa cells overexpressing AUF1s were resistant to H2O2‐induced senescence. Our results indicate that AUF1 is critical for H2O2‐induced p16 expression and cellular senescence. J. Cell. Biochem. 109: 1000–1005, 2010. © 2010 Wiley‐Liss, Inc.
Characterization of ATP‐induced cell death in the GL261 mouse glioma
Authors: Tamajusuku Alessandra S.K. , Villodre Emilly S. , Paulus Romela , Coutinho‐Silva Robson , Battasstini Ana M.O. , Wink Márcia R. , Lenz Guido 
Published: January 2010

AbstractGliomas have one of the worst prognosis among cancers. Their resistance to cell death induced by endogenous neurotoxic agents, such as extracellular ATP, seems to play an important role in their pathobiology since alterations in the degradation rate of extracellular ATP drastically affects glioma growth in rats. In the present work we characterized the mechanisms of cell death induced by extracellular ATP in a murine glioma cell line, GL261. ATP and BzATP, a P2X7 agonist, induced cell death at concentrations that are described to activate the P2X7 receptor in mouse. oATP, an antagonist of P2X7, blocked the ATP‐induced cell death. Agonists of purinergic receptors expressed in GL261 such as adenosine, ADP, UTP did not cause any cell death, even at mM concentrations. A sub‐population of cells more sensitive to ATP expressed more P2X7 when compared to a less sensitive subpopulation. Accordingly, RNA interference of the P2X7 receptor drastically reduced ATP‐induced cell death, suggesting that this receptor is necessary for this effect. The mechanism of ATP‐induced cell death is predominantly necrotic, since cells presented shrinkage accompanied by membrane permeabilization, but not apoptotic, since no phosphatidylserine externalization or caspase activity was observed. These data show the importance of P2X7 in ATP‐induced cell death and shed light on the importance of ATP‐induced cell death in glioma development. J. Cell. Biochem. 109: 983–991, 2010. © 2010 Wiley‐Liss, Inc.
Activation and induction of cytosolic phospholipase A2by IL‐1β in human tracheal smooth muscle cells: Role of MAPKs/p300 and NF‐κB
Authors: Lee Chiang‐Wen , Lee I‐Ta , Lin Chih‐Chung , Lee Hui‐Chun , Lin Wei‐Ning , Yang Chuen‐Mao 
Published: January 2010

AbstractCytosolic phospholipase A2(cPLA2) plays a pivotal role in mediating agonist‐induced arachidonic acid (AA) release for prostaglandin (PG) synthesis during inflammation triggered by IL‐1β. However, the mechanisms underlying IL‐1β‐induced cPLA2expression and PGE2synthesis in human tracheal smooth muscle cells (HTSMCs) remain unknown. IL‐1β‐induced cPLA2protein and mRNA expression, PGE2production, or phosphorylation of p42/p44 MAPK, p38 MAPK, and JNK1/2, which was attenuated by pretreatment with the inhibitors of MEK1/2 (U0126), p38 MAPK (SB202190), and JNK1/2 (SP600125) or transfection with siRNAs of MEK1, p42, p38, and JNK2. IL‐1β‐induced cPLA2expression was also inhibited by pretreatment with a NF‐κB inhibitor, helenalin or transfection with siRNA of NIK, IKKα, or IKKβ. IL‐β‐induced NF‐κB translocation was blocked by pretreatment with helenalin, but not U0126, SB202190, and SP600125. In addition, transfection with p300 siRNA blocked cPLA2expression induced by IL‐1β. Moreover, p300 was associated with the cPLA2promoter, which was dynamically linked to histone H4 acetylation stimulated by IL‐1β. These results suggest that in HTSMCs, activation of MAPKs, NF‐κB, and p300 are essential for IL‐1β‐induced cPLA2expression and PGE2secretion. J. Cell. Biochem. 109: 1045–1056, 2010. © 2010 Wiley‐Liss, Inc.