bluuebunny/crossref_metadata_2025_split huggingface dataset:
TIM‐3 Expression in Endometriosis
Authors: Lourenço Reis José , Martins Catarina , Ângelo‐Dias Miguel , Rosa Natacha Nurdine , Borrego Luís Miguel , Lima Jorge 
Published: June 2024

ABSTRACTProblemEndometriosis is a prevalent chronic gynecological disease linked to immune dysfunction. The protein T‐cell immunoglobulin and mucin domain‐containing protein 3 (TIM‐3) plays a crucial role in immune system balance. Malfunction of TIM‐3 may result in excessive immune activation and inflammatory tissue damage. Given TIM‐3's established role in the development of cancer and autoimmune diseases, we decided to study its role in women suffering from endometriosis.Study MethodWe included a total of 62 female patients, all of whom had undergone laparoscopic surgery. Of these, 47 had endometriosis and 15 did not. During surgery, we collected peritoneal fluid (PF) and peripheral blood (PB) samples from every patient for analysis using flow cytometry. To mark the samples, we used a panel of monoclonal antibodies and examined TIM‐3 expression in their immune cells.ResultsEndometriosis patients in PB demonstrated a significantly lower percentage of CD56+ T cells with TIM‐3 expression. As endometriosis progressed through its stages, this expression lessened. This decrease was particularly notable in women with stage III/IV endometriosis. Additionally, both women diagnosed with intestinal endometriosis and those with recent endometriosis diagnoses showed a significantly reduced percentage of CD56+ T cells expressing TIM‐3.ConclusionsPatients with endometriosis exhibit diminished TIM‐3 expression within circulating T cells. This warrants further investigation to discern whether it contributes to the progression of endometriosis, potentially through the amplification of autoreactive T cells and inflammation.
Rural physician–community engagement: Building, supporting and maintaining resilient health care strategies in three rural Canadian communities
Authors: Bland Alexandra , Meyer Anthon , Orrantia Eliseo , Hale Ilona , Grzybowski Stefan 
Published: June 2024

AbstractObjectiveTo explore rural physician–community engagement through three case studies in order to understand the role that these relationships can play in increasing community‐level resilience to climate change and ecosystem disruption.DesignQualitative secondary case study analysis.SettingThree Canadian rural communities (BC n = 2, Ontario n = 1).ParticipantsRural family physicians and community members.MethodsTwenty‐eight semi‐structured virtual interviews, conducted between November 2021 and February 2022, were included. Communities were selected from the larger data set based on data availability, level of physician engagement and demographic factors. Thematic analysis was completed in NVivo using deductive coding.Main FindingsThe presented qualitative case studies shed light on the strategies employed by physicians to establish and foster relationships within rural communities during challenging circumstances. In Community A, the implementation of a Primary Care Society (PCS) not only addressed physician shortages but also facilitated the development of strong continuity of care through proactive recruitment efforts. Community B showcased the adoption of an ‘intentional physician community’ model, emphasising collaboration and community consultation, resulting in effective communication of public health directives and innovative interdisciplinary action during the COVID‐19 pandemic. In Community C, engaged physicians and community advocates are aligned to contribute to the long‐term sustainability of the rural community, particularly in the context of food security and climate change vulnerabilities.ConclusionThese findings underscore the significance of trust building, transparent communication and collaboration in addressing health care challenges in rural areas and emphasise the need to recognise and support physicians as agents of change.
“Creating shared value”: Time for a normative extension?
Authors: Schwartz Mark S. 
Published: June 2024

AbstractPorter and Kramer's “creating shared value” (CSV) proposal has achieved significant penetration into both the academic and corporate communities. Building on other critiques of CSV, this paper assesses whether the CSV framework, notwithstanding its popularity, currently possesses an appropriate and adequate theoretical foundation to represent an overarching normative framework for the entire business and society field. The analysis does so by comparing CSV with a series of other dominant business and society approaches including corporate social responsibility, business ethics, stakeholder management, sustainability, and corporate citizenship. The analysis finds that while CSV does address the fundamental business and society normative requirement that business activities should contribute to sustainable net societal value, it currently fails to adequately incorporate the equally important notions of (i) appropriately balancing stakeholder interests with those of the corporation's shareholders, as well as (ii) demonstrating sufficient accountability (i.e., taking responsibility) by properly reporting on and addressing any negative impacts resulting from the firm's activities. The paper concludes with a revised and expanded restatement of the CSV concept, which attempts to take into account and address its current theoretical limitations in order to enhance its appeal as an overarching business and society normative paradigm.
Belantamab mafodotin concentration–QTc relationships in patients with relapsed or refractory multiple myeloma from the DREAMM‐1 and ‐2 studies
Authors: Jewell Roxanne C. , Mills Richard J. , Farrell Colm , Visser Sandra A. G. 
Published: June 2024

AbstractAimsTo evaluate relationships between plasma concentrations of belantamab mafodotin, total monoclonal antibody, and its payload and changes in electrocardiogram (ECG) parameters in patients with relapsed or refractory multiple myeloma from the DREAMM‐1 and DREAMM‐2 studies.MethodsHysteresis plots and linear regression analyses of pharmacokinetic (PK) analyte (belantamab mafodotin, total monoclonal antibody, and cytotoxic cysteine‐maleimidocaproyl monomethyl auristatin F payload) concentrations vs. time‐matched ECG parameters (absolute/change from baseline in QT interval corrected for RR interval [QTc/ΔQTc] and QT interval corrected for heart rate by Fridericia's formula [QTcF/ΔQTcF]) were performed. Concentrations of PK analyte required for a 10‐ms increase in QTc in DREAMM‐2 were calculated via simulation, as was the probability of ΔQTc/ΔQTcF exceeding 10 ms for the expected Cmax of PK analyte concentrations associated with the doses (2.5 and 3.4 mg/kg) administered in DREAMM‐2.ResultsTime‐matched PK and ECG data from 290 patients (DREAMM‐1, n = 73; DREAMM‐2, n = 217) were analysed. Hysteresis plots did not clearly indicate any concentration‐related prolongation in QTc or QTcF; regression analyses indicated a very small rate of increase in ΔQTc and ΔQTcF with increasing concentrations of PK analytes. Calculated concentrations of PK analyte required for a 10‐ms prolongation in QTc were higher than the maximum analyte concentrations observed following treatment with belantamab mafodotin in DREAMM‐2; the probability that each dose would prolong ΔQTc and ΔQTcF by >10 ms was 0 and <0.25%, respectively.ConclusionThis study of belantamab mafodotin and its payload did not provide evidence of QT prolongation in patients with relapsed or refractory multiple myeloma at clinically relevant doses.
Cooperative networking and information processing system of wireless communication UAV under the background of intelligent service
Authors: Chen Zhiyong 
Published: June 2024

AbstractIn order to solve the huge impact of the digital information age on many technical and industrial fields, a periodic fast search genetic algorithm is proposed. Based on the reconnaissance mission, this paper introduces the common allocation strategy into mission planning, and constructs the mathematical model of multi unmanned aerial vehicle (UAV) cooperative reconnaissance mission planning decision‐making. The proposed periodic fast search genetic algorithm is used to solve the problem of multi UAV cooperative reconnaissance mission planning. In 2020, the industry growth rate of global UAV technology expenditure was as high as 30.6%, and the compound growth rate of UAV in China reached 63.5%, which is enough to see the great prospect of the integrated development of UAV technology and different industries. The experiment evaluates the log verification module of UAV by comparing the two data structures of Merkle tree and linear, and the time and memory overhead of storing and verifying logs, which shows the effectiveness of the log verification scheme in this paper.
Exploring society‐to‐cells resilience among community members: A multidisciplinary study in outpatient clinics with diverse participant demographics
Authors: Ibrahim Ateya Megahed 
Published: June 2024

AbstractAimThis study explores and understands community members' resilience in outpatient clinics, considering various dimensions and types of resilience.DesignA cross‐sectional study.MethodsThis study was conducted in Saudi outpatient clinics from September to December 2023 and included 384 individuals chosen through systematic random sampling. Various tools were used, such as Social Cohesion and Trust Scale, Community Resilience Assessment Tool, Community Assessment of Psychic Experiences, Environmental Resilience Assessment, Economic Resilience Index, Connor‐Davidson Resilience Scale, Brief Resilience Scale, Resilience Scale for Adults and Healthcare Resilience Index.ResultsParticipants displayed a robust overall resilience level, as indicated by Total Connor–Davidson Resilience Scale score of 63.0 ± 9.0. Additionally, they demonstrated commendable levels of resilience in Total Brief Resilience Scale (56.04 ± 8.6), Resilience Scale for Adults (82.5 ± 7.2) and Healthcare Resilience Index (45.8 ± 5.5). These findings offer significant insights into psychological and emotional well‐being of the study population, highlighting their adaptive capacities and coping mechanisms across various life domains.ConclusionThis study provides valuable insights into the multidimensional nature of resilience in outpatient settings. The cross‐sectional design sets the groundwork for future longitudinal investigations, highlighting the need for a holistic approach to understanding and promoting resilience.ImpactThis study holds immediate implications for participants and their communities. It underscores the adaptive capacities and coping mechanisms prevalent in the outpatient population by revealing commendable resilience levels. This insight enhances individuals' psychological and emotional well‐being, contributing positively to the overall resilience and communal strength. Additionally, this study sheds light on how resilience among community members in Saudi Arabia relates to international advanced nursing communities, providing insight into their work.Patient or Public ContributionPatients who have received outpatient services in the past 6 months were purposively chosen to ensure a diverse representation across age, gender and socio‐economic backgrounds in this study.
Longitudinal observation of tRNA‐derived fragments profiles in gestational diabetes mellitus and its diagnostic value
Authors: Hu Yifang , Liu Yan , Shen Jun , Yin Lihua , Hu Xiaoxia , Huang Xiaolei , Chen Yingyuan , Zhang Yisheng 
Published: June 2024

AbstractBackgroundGestational Diabetes Mellitus (GDM) poses significant risks to maternal and fetal health. Current diagnostic methods based on glucose tolerance tests have limitations for early detection. tRNA‐derived small RNAs (tsRNAs) have emerged as potential molecular regulators in various diseases, including metabolic disorders. However, the diagnostic value of tsRNAs in plasma for early GDM or postpartum remains unclear.MethodsThis longitudinal study profiled the expression of tsRNAs across different gestational stages and postpartum in women with GDM (n = 40) and healthy control gestational women (HCs, n = 40). High‐throughput small RNA sequencing identified candidate tsRNAs, which were then validated and correlated with clinical biochemical markers such as fasting blood glucose (FBG), HOMA‐IR, and GHbA1c.ResultstRF‐1:32‐Val‐AAC‐1‐M6, tRF‐1:31‐Glu‐CTC‐1‐M2, and tRF‐1:30‐Gly‐CCC‐1‐M4 were consistently upregulated in the GDM group compared to HCs during the second trimester (p < 0.05). Only tRF‐1:31‐Glu‐CTC‐1‐M2 was highly expressed during the first trimester, and tRF‐1:30‐Gly‐CCC‐1‐M4 increased during postpartum. tRF‐1:31‐Glu‐CTC‐1‐M2 showed a significant correlation with FBG levels in the first trimester (R = 0.317, p = 0.047). The expression of tRF‐1:30‐Gly‐CCC‐1‐M4 was significantly correlated with HOMA‐IR (r = 0.65, p < 0.001) and GHBA1c (r = 0.33, p = 0.037) during postpartum. A joint diagnostic model incorporating tsRNAs expression and clinical markers demonstrated enhanced predictive power for GDM (ROC AUC = 0.768).ConclusionOur results revealed distinct expression patterns of specific tsRNAs in GDM, showcasing their correlation with key metabolic parameters. This underscores their promising role as biomarkers for early prediction and diagnosis of GDM. The integration of tRFs into a composite biomarker panel holds the potential to improve clinical outcomes by enabling personalized risk assessment and targeted interventions.
A preliminary biocontrol study: Isolation, characterization and genomic analysis of lytic phage TE against Pseudomonas syringae pv. syringae
Authors: Selcuk Emine , Erdogdu Berna , Ozbek Tulin 
Published: June 2024

AbstractTraditionally, apricot trees suffering from bacterial canker disease caused by Pseudomonas syringae pv. syringae were treated in orchards through the application of antibiotics or copper‐based drugs. However, considering the disadvantages of the long‐term use of chemical pesticides and antibiotics, there has been an increasing interest in phages, which have a lytic effect on bacteria, as biopesticides in recent years. In this study, we conducted a comprehensive examination of phage TE, isolated from wastewater in Turkey, from this perspective. Through scanning electron microscopy and phylogenetic analysis, we classified phage TE as belonging to the order Caudovirales and the family Strabovoridae. The phage TE exhibited a robust host lysis efficiency, with 280 infectious phage particles produced per bacterial cell in just 15 min. In experimental trials, treatment with phage TE resulted in an impressive 82% inhibition of bacterial canker disease in apricot leaves, highlighting its effectiveness as a phage therapy agent. Furthermore, phage TE exhibited long‐term stability when stored at 4°C without cryoprotectant and displayed tolerance to a wide temperature (−20°C to 50°C) and pH (2–12) range but its tolerance to UV‐C was very low. The genome and bioinformatics analyses of P. syringae phage TE have identified distinct protein level differences between other phages of the same species, despite sharing similarities at the nucleotide level. These findings suggest the potential use of phage TE as a biocontrol agent, offering a promising solution to control apricot bacterial canker.
Fusarium fujikuroi as a potential biocontrol agent of the parasitic weed Phelipanche aegyptiaca in tomato
Authors: Cignitas Esra , Basbagci Gurkan , Sulu Gorkem , Kitis Yasin Emre 
Published: June 2024

AbstractPhelipanche aegyptiaca (Pers.) Pomel, commonly known as Egyptian broomrape, is an obligate root parasite, infesting solanaceous crops mainly in Mediterranean countries. Certain antagonistic fungi could be effectively used as a biocontrol agent for this parasitic plant. In this study, three fungal isolates (F1, F2 and F3) were obtained from P. aegyptiaca seeds covered with mycelium. The isolates were characterized based on morphological features and ITS and TEF sequence analysis. They were classified as belonging to Fusarium fujikuroi with a sequence similarity of 99.80% and 100%, respectively. The pathogenicity of the isolates on P. aegyptiaca and tomato were determined. In a P. aegyptiaca seed assay, all isolates allowed seed germination with delayed radicle development. Disease severity among the isolates ranged from 40%–70% on the basis of radicle necrotic area. The F1 isolate caused more than 40% disease on the spiders and tubercles in a polyethylene bag assay. The average of the total of spider and tubercle mortality caused by the F1 isolate was 63.5% at 9 days after inoculation. Moreover, the F1 isolate was considered non‐pathogen on tomato with 6.66% disease severity after root inoculation. To the best of our knowledge, this is the first report of F. fujikuroi on P. aegyptiaca seed. Pending further development, the F1 isolate could have potential for the biocontrol of P. aegyptiaca in the future.
The complex hexaploid oil‐Camellia genome traces back its phylogenomic history and multi‐omics analysis of Camellia oil biosynthesis
Authors: Zhu Huaguo , Wang Fuqiu , Xu Zhongping , Wang Guanying , Hu Lisong , Cheng Junyong , Ge Xianhong , Liu Jinxuan , Chen Wei , Li Qiang , Xue Fei , Liu Feng , Li Wenying , Wu Lan , Cheng Xinqi , Tang Xinxin , Yang Chaochen , Lindsey Keith , Zhang Xianlong , Ding Fang , Hu Haiyan , Hu Xiaoming , Jin Shuangxia 
Published: June 2024

SummaryOil‐Camellia (Camellia oleifera), belonging to the Theaceae family Camellia, is an important woody edible oil tree species. The Camellia oil in its mature seed kernels, mainly consists of more than 90% unsaturated fatty acids, tea polyphenols, flavonoids, squalene and other active substances, which is one of the best quality edible vegetable oils in the world. However, genetic research and molecular breeding on oil‐Camellia are challenging due to its complex genetic background. Here, we successfully report a chromosome‐scale genome assembly for a hexaploid oil‐Camellia cultivar Changlin40. This assembly contains 8.80 Gb genomic sequences with scaffold N50 of 180.0 Mb and 45 pseudochromosomes comprising 15 homologous groups with three members each, which contain 135 868 genes with an average length of 3936 bp. Referring to the diploid genome, intragenomic and intergenomic comparisons of synteny indicate homologous chromosomal similarity and changes. Moreover, comparative and evolutionary analyses reveal three rounds of whole‐genome duplication (WGD) events, as well as the possible diversification of hexaploid Changlin40 with diploid occurred approximately 9.06 million years ago (MYA). Furthermore, through the combination of genomics, transcriptomics and metabolomics approaches, a complex regulatory network was constructed and allows to identify potential key structural genes (SAD, FAD2 and FAD3) and transcription factors (AP2 and C2H2) that regulate the metabolism of Camellia oil, especially for unsaturated fatty acids biosynthesis. Overall, the genomic resource generated from this study has great potential to accelerate the research for the molecular biology and genetic improvement of hexaploid oil‐Camellia, as well as to understand polyploid genome evolution.
Long‐Term Outcomes After Lung Transplantation in Children With Intellectual Disabilities
Authors: Lee James Y. , Mullis Danielle M. , Zawadzki Roy S. , Nilkant Riya , Kuhan Sangkavi , Kidambi Sumanth , Sharir Amit , Ma Michael 
Published: June 2024

ABSTRACTBackgroundThe United Network for Organ Sharing (UNOS) started recording data on intellectual disability status in 2008. This study aimed to characterize the long‐term outcomes for children with intellectual disabilities (IDs) undergoing lung transplantation.MethodsAll pediatric patients (under 18 years old) undergoing bilateral lung transplantation were identified using the UNOS database. The patients were grouped into the following categories: no cognitive delay, possible cognitive delay, and definite cognitive delay. The primary endpoint was graft survival at 3‐year posttransplantation. Multivariate Cox proportional hazards modeling was used to estimate the independent effect of cognitive disability on graft survival.ResultsFive hundred four pediatric patients who underwent lung transplantation between March 2008 and December 2022 were retrospectively analyzed. 59 had a definite cognitive delay (12%), 23 had a possible delay (5%), and 421 had no delay (83%). When comparing these three groups, there was no significant difference in 60‐day graft survival (p = 0.4), 3‐year graft survival (p = 0.6), 3‐year graft survival for patients who survived at least 60‐day posttransplantation (p = 0.9), distribution of causes of death (p = 0.24), nor distribution treatment of rejection within 1‐year posttransplantation (p = 0.06).ConclusionsIntellectual disability does not impact long‐term outcomes after bilateral lung transplantation. Intellectual disability should not be a contraindication to bilateral lung transplantation on the basis of inferior graft survival.
Rab GTPases, Active Members in Antigen‐Presenting Cells, and T Lymphocytes
Authors: Moreno‐Corona Nidia Carolina , de León‐Bautista Mercedes Piedad , León‐Juárez Moises , Hernández‐Flores Araceli , Barragán‐Gálvez Juan Carlos , López‐Ortega Orestes 
Published: June 2024

ABSTRACTProcesses such as cell migration, phagocytosis, endocytosis, and exocytosis refer to the intense exchange of information between the internal and external environment in the cells, known as vesicular trafficking. In eukaryotic cells, these essential cellular crosstalks are controlled by Rab GTPases proteins through diverse adaptor proteins like SNAREs complex, coat proteins, phospholipids, kinases, phosphatases, molecular motors, actin, or tubulin cytoskeleton, among others, all necessary for appropriate mobilization of vesicles and distribution of molecules. Considering these molecular events, Rab GTPases are critical components in specific biological processes of immune cells, and many reports refer primarily to macrophages; therefore, in this review, we address specific functions in immune cells, concretely in the mechanism by which the GTPase contributes in dendritic cells (DCs) and, T/B lymphocytes.
Cutibacterium acnes contamination does not enhance the proinflammatory profile of platelet concentrates
Authors: Kumaran Dilini , Ramirez‐Arcos Sandra 
Published: June 2024

AbstractBackgroundCutibacterium acnes, a common anaerobic platelet concentrate (PC) contaminant, has been associated with rare mild adverse transfusion reactions and is often considered a harmless commensal. Notably, C. acnes can cause chronic infections and has been shown to induce the release of proinflammatory cytokines by immune cells. Since elevated concentrations of proinflammatory factors in PCs have been linked to noninfectious adverse reactions, this study aimed to assess whether C. acnes could elicit the release and accumulation of proinflammatory factors during PC storage, thereby enhancing the risk of such reactions.Study Design/MethodsFour ABO‐matched buffy coat PCs were pooled and split into six units, each were inoculated with either saline (negative control), a Staphylococcus aureus isolate (positive control, 30 colony forming units [CFU]/unit), or four C. acnes PC isolates (10 CFU/mL) and stored at 20–24°C with agitation. Bacterial counts, platelet activation, and concentration of proinflammatory factors were assessed on days 0, 3, and 5. N = 3.ResultsC. acnes counts remained stable, while S. aureus proliferated reaching 108CFU/mL by the end of PC storage. By day 5, no significant differences in platelet activation or proinflammatory cytokine profiles were observed in C. acnes‐contaminated PCs compared to the negative control (p > .05), while there was a significant increase (p ≤ .05) in sCD40L concentration (day 3), and platelet activation and IL‐8 concentration (day 5) in S. aureus‐contaminated units.DiscussionC. acnes contamination does not promote the accumulation of proinflammatory factors in the absence of proliferation during storage and may not enhance the risk of inflammatory reactions when transfused to patients.