bluuebunny/crossref_metadata_2025_split huggingface dataset:
Downregulation of PIF1 induce DNA damage and inhibit ovarian cancer cell proliferation via RAD51
Authors: Zhou Qi-Yin , Hua Yu-Xin , Sun Qing-Qing , Zou Shang-Pu , Guo Jia-Lin , Ni Meng-Zhen , Zhang Shuo , Liu Sheng-Bing , Guo Yan-Jun , Ao Lei , Che Xuan , Zhang Xian-Chao , Heger Michal , Zheng Xin , Wu Zhong-Jie , Wang Xiao-Min , Cheng Shu-Qun , Pan Wei-Wei 
Published: June 2024

Abstract

Background
PIF1 helicase (5ʹ→3ʹ DNA helicase) is a member of helicase superfamily 1. It has unwinding activity and plays a crucial role in maintaining genome stability and coordinating DNA damage repair processes. Overexpression of PIF1 is common in several cancers; however, its role in ovarian cancer remains unclear. This study aimed to elucidate the regulatory role of PIF1 in ovarian cancer and explore its mechanism.
Results
Analysis of patient samples and public database datasets revealed a negative correlation between PIF1 overexpression and the overall survival rate of the patients. We found through molecular biology experiments and xenograft tumor models in nude mice that CRISPR/Cas9-mediated PIF1 partial knockdown in ovarian cancer cell lines significantly inhibited proliferation and clonogenicity, promoted senescence, and induced G2 cell cycle arrest. Moreover, PIF1 partial deficiency enhanced DNA damage in ovarian cancer cells, particularly sensitive to cisplatin. RAD51 serves as a central scaffold protein for homologous recombination repair and is crucial for timely and accurate DNA repair. We observed that PIF1 partial knockdown resulted in significant reduction of RAD51 in ovarian cancer cells. Notably, RAD51 overexpression in PIF1 partially deficient ovarian cancer cells rescued cell proliferation and DNA damage by increasing PIF1 expression. Immunofluorescence revealed the co-localization of EGFP-PIF1 and RAD51 in the cell nucleus, suggesting that the interaction between PIF1 and RAD51 may regulate the DNA damage response and cell survival in ovarian cancer cells.
Conclusions
Our study revealed that PIF1 is a druggable target for inducing DNA damage in ovarian cancer cells and provides insights into the potential synergistic mechanisms of action between PIF1 and RAD51 in ovarian cancer therapy.
Research on Shared Bicycle Prediction Using Gated Graph Convolutional Networks with Multi-Feature Edge Weights
Authors: Guo Hebin , Li Kexin , Rou Yutong 
Published: June 2024

Abstract

This study proposes an hourly demand prediction method based on a multi-feature edge-weighted gated graph convolutional network to address the imbalance in station borrowing and returning demands, as well as low station utilization in bike-sharing systems. By employing graph convolutional neural networks to capture spatial relationships between stations and utilizing gating mechanisms to integrate current and historical information, it captures the long-term dependency of time series data. Creatively, it combines three single edge-weight features—station distance, time, and correlation value—into a multi-feature edge-weighted input model graph structure, enhancing the accuracy in reflecting traveler behavior characteristics. Additionally, the study considers not only temporal and spatial factors but also incorporates traveler features as node inputs to the model. Using bike-sharing trip data from Jersey City in 2020, the study employs isolation forest algorithm for outlier detection, followed by feature dependency analysis to reveal the impact of time, space, and traveler features on demand. Moreover, it accounts for the seasonal influence on bike-sharing trips by dividing the dataset into different seasons and conducting unified research on similar seasons. Results demonstrate that the multi-feature edge-weighted gated graph convolutional neural network achieves an MAE of 0.52 and MSE of 0.906 for the spring and autumn seasons, and an MAE of 0.296 and MSE of 0.594 for the summer and winter seasons, outperforming baseline models and single-feature edge-weighted predictive performance.
Atomically resolved imaging of radiation-sensitive metal-organic frameworks via electron ptychography
Authors: Han Yu , Li Guanxing , Xu Ming , Tang Wen-Qi , Liu Ying , Chen Cailing , Zhang Daliang , Liu Lingmei , Ning Shoucong , Zhang Hui , Gu Zhi-Yuan , Lai Zhiping , Muller David 
Published: June 2024

Abstract

Electron ptychography, recognized as an ideal technique for low-dose imaging, consistently achieves deep sub-angstrom resolution in low-dimensional materials at electron doses of several thousand electrons per square angstrom (e-/Å2). Despite its proven efficacy, the application of electron ptychography at even lower doses—necessary for materials highly sensitive to electron beams—raises questions regarding its feasibility and the attainable resolution under such stringent conditions. Herein, we demonstrate the successful implementation of electron ptychography reconstruction at an unprecedentedly low electron dose of ~100 e-/Å2, for metal-organic frameworks (MOFs), which are known for their extreme sensitivity. The reconstructed images, achieving a resolution of ~2 Å, clearly resolve organic linkers, metal clusters, and even atomic columns within these clusters, while unravelling various local structural features in MOFs, including missing linkers, extra clusters, and surface termination modes. By combining the findings from simulations and experiments, we have identified that employing a small convergence semi-angle during data acquisition is crucial for effective iterative ptychographic reconstruction under such low-dose conditions. This important insight advances our understanding of the rapidly evolving electron ptychography technique and provides a novel approach to high-resolution imaging of various sensitive materials.
Vinasse treated with charcoal as a molasses diluent for ethanol fermentation
Authors: Madaleno Leonardo Lucas , Guimarães Camila Carla , De Paula Nádia Figueiredo , Teixeira Josiene Rocha 
Published: June 2024

ABSTRACT
The demand for new products derived from agro-industrial residues has increased recently. Furthermore, vinasse, a wastewater from ethanol production, needs treatment to be reused in the sugarcane industry, reducing industrial water consumption. This study performed vinasse filtration with charcoal from industrial sugarcane residues and used filtered molasses dilution in ethanolic fermentation. There were five treatments in randomized blocks with three repetitions. The treatments included deionized water and natural vinasse as positive and negative controls, respectively, and filtered vinasse from charcoal made from bamboo, sugarcane bagasse, and straw. Hence, fermentation for ethanol production was performed. Compared with natural vinasse, filtered vinasse with all types of charcoal showed lower soluble solids, total residual reducing sugars, higher ethanol concentrations, and greater fermentative efficiency. Filtered vinasse from bagasse and straw charcoals had efficiencies of 81.14% and 77.98%, respectively, in terms of ethanol production, which are close to those of deionized water (81.49%). In a hypothetical industry, vinasse charcoal filtration and charcoal regeneration should prevent 84.12% of water consumption from environmental resources. This process is feasible because it uses a product of sugarcane residue to treat wastewater and reduce industrial water consumption and vinasse disposal.
Examining direct and indirect flood damages in residential and business sectors through an empirical lens
Authors: Kabirzad S. A. , Rehan B. M. , Zulkafli Z. , Yusuf B. , Hasan-Basri B. , Toriman M. E. 
Published: June 2024

ABSTRACT
Investment to reduce flood risk for social and economic wellbeing requires quantitative evidence to guide decisions. Direct and indirect flood damages at individual household and business building levels were assessed in this study using multivariate analysis with three groups of flood damage attributes, i.e., flood characteristics, socioeconomic conditions, and building types. A total of 172 and 45 respondents from residential and commercial buildings were gathered through door-to-door interviews at areas in Peninsular Malaysia that were pre-identified to have frequently flooded. Two main findings can be drawn from this study. First, flood damage is greatly contributed by high-income households and businesses, despite them being less exposed to floods than low-income earners. This supports the current use of mean economic damage in engineering-based flood intervention analysis. Second, indirect damages increase with the increase in family size, indicating the importance of strengthening preparedness and social support to those with great social responsibility. Overall, the study highlights the importance of holistic flood management accounting for both direct and indirect losses.
Synthesis, Characterization, and Stability Optimization of Ibuprofen Cocrystals Employing Various Hydrophilic Polymers
Authors: Hassan Ayesha , Khan Jamshaid Ali , Nasir Fazli , Shabir Hira , Hannan Peer Abdul , Ullah Rahim , Jan Afnan , Khalid Asaad , Khan Ajmal , Al-Harrasi Ahmed 
Published: June 2024

Background:
Cocrystals are an efficient way for the delivery of low soluble drugs but when dissolved
they rapidly disproportionate. To formulate the cocrystals in tablets, cocrystals must be stabilized. In
this study ibuprofen-nicotinamide (IBU-NIC) cocrystals were synthesized initially by slow solvent evaporation
and for bulk production by fast solvent evaporation techniques.

Methods:
The cocrystals were characterized by powder X-ray diffraction (PXRD), Fourier transform infrared
spectrophotometer (FTIR), differential scanning calorimetry (DSC), thermogravimetric analysis (TGA), and
optical microscopy. The ibuprofen cocrystals showed greater solubility compared to the parent drug.

Result:
Intrinsic dissolution data was utilized for efficacious screening of tablet formulations. Using hydrophilic
polymers at a ratio of 6:1 (polymer to IBU-NIC cocrystal ratio), hydroxypropyl methylcellulose (F1),
polyvinylpyrrolidone (PVP) K-30 (F2) and PVP K-90 (F3), three tablet formulations were prepared that stabilized
cocrystals during dissolution. The drug release profiles after 60 minutes from formulations F1 (92.30), F2
(98.54), F3 (99.88) were all higher compared to the marketed brand BRUFEN® F, (79.61%) in a simulated intestinal
media (p<0.001).

Conclusion:
Significant increase in the dissolution rate of cocrystal was observed with no phase change in all
formulations.
Oleogels for the Promotion of Healthy Skin Care Products: Synthesis and Characterization of Allantoin Containing Moringa-based Oleogel
Authors: Yaqoob Nazia , Imtiaz Fazeelat , Shafiq Nusrat , Rehman Saima , Munir Huma , Bourhia Mohammed , Almaary Khalid S. , Nafidi Hiba-Allah 
Published: June 2024

Background:
Oleogelation is an efficient and emerging approach for obtaining biocompatible
and biodegradable elastic semisolid crystals to be used in various cosmetic and
pharmaceutical formulations. Recently, drug incorporation in oil structuring has been a promising
strategy under consideration due to the effectiveness of this method. Plant oils have very
beneficial characteristics for skin care and wound healing due to the presence of certain antioxidants.

Methods:
In this study, the oleogels of Moringa oleifera seed oil with natural polysaccharides,
including pectin, chitosan, and xanthan gum, were prepared using the emulsion template method.
Moringa oil was selected because it can hydrate and moisturize the skin and has great antioxidant
activity. Also, the natural polysaccharides, i.e., pectin and chitosan, exhibited good gelling properties.
Allantoin, which is a wound healer and eucalyptus leaf oil with antioxidant potential, was
incorporated into the emulsion-based-oleogels to enhance the antioxidant and antimicrobial activity
of the oleogels.

Results:
Allantoin and eucalyptus-loaded oleogels exhibited good antibacterial activity against
E. coli. The FTIR spectra of moringa-based oleogels in the range between 3226-3422 cm-1 indicate
the presence of hydrogen bonding in oleogels.

Conclusion:
The antioxidant potential of allantoin and eucalyptus-containing oleogel was maximized,
and an IC50 value of 0.9719 μM was found. Maximum release of allantoin from oleogel
was observed in the first hour.
Unravelling the Microbiome's Role in Healing Diabetic Wounds
Authors: Dhankhar Sanchit , Garg Nitika , Chauhan Samrat , Saini Monika , Singh Thakur Gurjeet , Singh Randhir 
Published: June 2024

Abstract::
The process of wound healing is intricate and requires close coordination; any disruption
to this process can have catastrophic results. It is hypothesized that chronic wounds that do
not heal or that cease healing entirely can be caused by a combination of host factors and bacteria
that are present in a wound bed or wound bed environment. There is currently a lack of understanding
regarding the role that the cutaneous microbiome plays in the healing process of
wounds, despite the fact that methods that do not rely on culture have revealed the role that the
gut microbiome plays in human health and illness. In order to keep the host immune system in
check, protect the epithelial barrier function, and ward off harmful microbes, skin commensals
play a crucial role. This review compiles the research on the effects of microbiome modifications
on wound healing and tissue regeneration from both clinical and pre-clinical investigations on a
variety of chronic skin wounds. It is now clear that human skin commensals, symbionts, and
pathogens all play a part in the inflammatory response, which in turn suggests a number of ways
to treat wounds that are infected and not healing. To fully understand the function of the human
skin microbiome in both short-term and long-term wound healing, additional study is required to
reconcile the conflicting and contentious results of previous investigations.
Frontiers of Plant-derived Exosomes from Research Methods to Pharmaceutical Applications in Plant-based Therapeutics
Authors: Fu Wenshang , Zhang Pingli , Wang Wei , Du Mengdie , Ni Rui , Sun Yongshun 
Published: June 2024

Abstract:
Exosomes have emerged as critical mediators of intercellular communication and various
physiological processes between cells and their environment. These nano-sized vesicles have
been extensively investigated and confirmed to exhibit multifunctionality in animal systems. In
particular, they participate in intercellular signaling, influence disease progression, and exhibit biological
activity. However, Plant-Derived Exosomes (PDEs), especially therapeutic PDEs, have received
relatively limited attention in the past few decades. Recent studies have demonstrated that
PDEs are involved in signaling molecule transport in addition to intercellular communication, as
they serve as functional molecules in the cellular microenvironment. This characteristic highlights
the immense potential of PDEs for a wide array of applications, including antioxidation, anti-inflammation,
tumour cell elimination, immune modulation, and tissue regeneration. In addition,
PDEs hold substantial promise as efficient drug carriers, enhancing the stability and bioavailability
of therapeutic agents and consequently enabling targeted delivery to specific cells or tissues.
Therefore, PDEs may serve as effective tools for drug delivery and the treatment of various diseases.
This comprehensive review provides an overview of recent studies on therapeutic PDEs, focusing
on their extraction, isolation, characterization methods, biological activities, and application
prospects. It summarises the research progress of exosome-like nanovesicles derived from medicinal
plants, with a specific emphasis on traditional Chinese medicine, and highlights their importance
in disease treatment and nanoparticle delivery. The main objective is to accelerate the clinical
translation of these nanovesicles while providing novel approaches and methodologies for the
research and development of innovative drugs.
24 Factorial Design Formulation Optimization and In vitro Characterization of Desloratadine Nanosuspension Prepared Using Antisolvent Precipitation
Authors: El-Sebaiy Mohamed T. , Alyami Mohammad H. , Alyami Hamad S. , Kamal Mohammad Amjad , Eissa Noura , Balata Gehan , El-Nahas Hanan 
Published: June 2024

Introduction:
Desloratadine, a second-generation antihistaminic drug, is poorly watersoluble
and requires amelioration of the dissolution rate to improve its pharmacokinetics properties.

Method:
This study investigated the impact of polymer, surfactant types, and concentration on the
particle size, zeta potential, and dissolution efficiency of nanosuspensions formulated through the
solvent antisolvent precipitation method. To optimize the delivery of Desloratadine nanosuspension,
we used Minitab software and a 4-factor, 2-level full factorial design. Physicochemical properties
and drug release studies were conducted to evaluate the suggested nanosuspension formulations. The
optimization goals included minimizing particle size and zeta potential while maximizing dissolution
efficiencies.

Result:
The selected optimal nanosuspension demonstrated favourable values, including a particle
size of 478.63 ± 15.67 nm, a zeta potential of -36.24 ± 3.21 mV, and dissolution efficiencies in double
distilled water and buffer of 90.29 ± 3.75 % and 93.70 ± 3.67 %, respectively. The optimized
formulation was subjected to additional analysis using X-ray powder diffraction (XPRD), scanning
and transmission electron microscopy (SEM and TEM), and Fourier-transform infrared spectroscopy
(FTIR).

Conclusion:
The optimized nanosuspension formulation also underwent further studies under optimal
lyophilization conditions, revealing the effectiveness of mannitol as a cryoprotectant at a concentration
of 8%.
Role of Plant Materials with Anti-inflammatory Effects in Phytotherapy of Osteoarthritis
Authors: Geszke-Moritz Malgorzata , Nowak Gerard , Moritz Michał , Feist Barbara , Nycz Jacek E. 
Published: June 2024

:
Osteoarthritis (OA) is a common chronic articular degenerative disease characterized
by articular cartilage degradation, synovial inflammation/immunity, and subchondral bone lesions.
Recently, increasing interest has been devoted to treating or preventing OA with herbal
medicines. The mechanism of action of plant raw materials used in osteoarthrosis treatment is
well documented. They are sought after because of the high frequency of inflammation of the
knee joint among both elderly and young people engaged in sports in which their knee joints are
often exposed to high-stress conditions.

:
The purpose of this work was to present some most effective and safe plant medicines with
proven mechanisms of action that can help to alleviate the growing social problem of osteoarthrosis
caused in recent years.

:
A review of the available literature based primarily on the latest editions of ESCOP and EMA
monographs and the latest scientific papers has made it possible to select and propose medical management
of osteoarthrosis by ranking plant medicines according to their effectiveness.

:
Clinical studies of raw plant materials, such as Harpagophyti radix, Olibanum indicum, and Urticae
foliumet herba have indicated that these drugs should be considered the first choice in osteoarthrosis
treatment. The efficacy of Rosae pseudo-fructus, Salicis cortex, Filipendulae ulmariae
flos et herba, Ribis nigri folium, and externally applied Capsici fructus and Symphyti radix, has
also been proven by pharmacological studies. All the plant medicines mentioned in the paper have
been studied in detail in terms of their phytochemistry, which can help doctors in their decisionmaking
in the treatment of osteoarthrosis.
Chemistry or Hypoglycaemic Aspect of Thiazolidin-2,4-Dione Insight into
the Structure-activity Relationship
Authors: Kaur Parvinder , Kumar Nitin , Sharma Shikha , Banik Bimal Krishna 
Published: June 2024

Abstract::
Heterocyclic compounds that incorporate nitrogen and sulfur, particularly those within
the thiazole family, have garnered significant attention due to their versatile synthetic properties.
This interest arises from their abundance of biologically active natural products and their effectiveness
as potent agrochemicals and pharmaceuticals. Among these, the thiazolidin-2,4-dione
(TZD) motif plays a pivotal role in the biological functions of many important molecules. The
adaptability of the TZD scaffold is further heightened by the potential for substitutions at the third
and fifth positions, rendering it a widely employed moiety with diverse biological effects. TZD
analogs, bearing substitutions at these positions, exhibit a wide spectrum of biological activities,
with a notable emphasis on hypoglycemic effects attributed to enhanced insulin resistance
through the activation of the PPAR-γ receptor. This manuscript aims to present a comprehensive
review of research conducted on TZD derivatives as potential antihyperglycemic agents spanning
from 2010 to the current date. The review encompasses insights into their molecular mechanisms.
Thiazolidin-based chemicals have received significant attention as hypoglycemic agents and provided
information on patents granted for TZD analogs.
A Review on Current Prospective and Applications of Green Nanoparticles in Biomedical Applications
Authors: Bahadur Shiv , Agrawal Gaurav 
Published: June 2024

Abstract:
Green chemistry has been recognised as a new prominent field that endeavours to work
at the molecular level to achieve sustainability in the various area of pharmaceuticals. Sustainable
chemistry can be defined as the formation of chemical products which reduces the generation of
toxic and harmful substances. Hence, green chemistry may decrease pollution and risks of chemical
release into the biosphere. Green chemistry has various applications in specific fields like pharmaceutical
product development, analytical chemistry and chemical synthesis methods. The applications
of green chemistry and nanotechnology for drug delivery in pharmaceuticals can enhance
safety and effectiveness. The pollution resulting from conventional methods creates a requirement
for environmentally friendly methods. Green nanotechnology may be useful in several areas of
biomedical, such as plant extract may be used for the development of advanced novel nanoparticles
with the need for progress in biomedicine, antibacterial, nano-biotechnology, sensing and in
electrochemical, cosmetics, electronics, biosensors and other applications. The green chemistry approaches
may be implemented in various sectors of the pharmaceutical industry, such as biocatalysts,
green solvents, green nanotechnology, etc. Hence, the present review article highlights several
aspects of green chemistry and its applications in waste management and pollution control.
Further, the pharmaceutical application of green chemistry has been discussed.