bluuebunny/crossref_metadata_2025_split huggingface dataset:
The development of a rapid SYBR Green I-based quantitative PCR for detection of Duck circovirus
Authors: Wan Chunhe , Huang Yu , Cheng Longfei , Fu Guanghua , Shi Shao-hua , Chen Hongmei , Peng Chunxiang , Lin Fang , Lin Jiansheng 
Published: October 2011

Abstract
This report describes a one-step real-time polymerase chain reaction assay based on SYBR Green I for detection of a broad range of duck circovirus (DuCV). Align with all DuCV complete genome sequences and other Genus Circovirus download from the GenBank (such as goose circovirus, pigeon circovirus), the primers targets to the replicate gene of DuCV were designed. The detection assay was linear in the range of 1.31 × 102-1.31 × 107 copies/μL. The reaction efficiency of the assay using the slope (the slope was -3.349) and the Y-intercept was 37.01 from the linear equation was estimated to be 0.99 and the correlation coefficient (R2) was 0.993. A series of experiments were carried out to assess the reproducibility, sensitivity, and specificity of the assay, following by the low intra-assay and inter-assay CVs for CT values obtained with the standard plasmids. The intra-assay CVs were equal or less than 1.89% and the inter-assay CVs were equal or less than 1.26%. There was no cross-reaction occurred with nucleic acids extracted from RA (Riemerella anatipestifer), E. coli (Escherichia coli), Duck Cholera (Pasteurella multocida), Avian influenza virus, avian paramyxovirus, Muscovy duck parvovirus, Duck reovirus, Duck hepatitis A virus as control templates. The nucleic acids extracted from samples of healthy ducks were used as negative controls. The assay was specific and reproducible. The established real time PCR was used to detect 45 DuCV-negative samples, which were tested using conventional PCR under the developed optimal conditions, each 15 for embryonated eggs, non-embryonated budgerigar eggs, newly hatched duck, the mixture of the lung, liver, spleen which were analysis for the presence of DuCV DNA, to conform that whether the DuCV can be transmitted vertically. Meanwhile, no positive result was shown by the real-time PCR method. The SYBR Green I-based quantitative PCR can therefore be practically used as an alternative diagnostic tool and a screening method for ducks infected with duck circovirus.
A zebrafish transgenic model of Ewing’s sarcoma reveals conserved mediators of EWS-FLI1 tumorigenesis
Authors: Leacock Stefanie W. , Basse Audrey N. , Chandler Garvin L. , Kirk Anne M. , Rakheja Dinesh , Amatruda James F. 
Published: October 2011

SUMMARY
Ewing’s sarcoma, a malignant bone tumor of children and young adults, is a member of the small-round-blue-cell tumor family. Ewing’s sarcoma family tumors (ESFTs), which include peripheral primitive neuroectodermal tumors (PNETs), are characterized by chromosomal translocations that generate fusions between the EWS gene and ETS-family transcription factors, most commonly FLI1. The EWS-FLI1 fusion oncoprotein represents an attractive therapeutic target for treatment of Ewing’s sarcoma. The cell of origin of ESFT and the molecular mechanisms by which EWS-FLI1 mediates tumorigenesis remain unknown, and few animal models of Ewing’s sarcoma exist. Here, we report the use of zebrafish as a vertebrate model of EWS-FLI1 function and tumorigenesis. Mosaic expression of the human EWS-FLI1 fusion protein in zebrafish caused the development of tumors with histology strongly resembling that of human Ewing’s sarcoma. The incidence of tumors increased in a p53 mutant background, suggesting that the p53 pathway suppresses EWS-FLI1-driven tumorigenesis. Gene expression profiling of the zebrafish tumors defined a set of genes that might be regulated by EWS-FLI1, including the zebrafish ortholog of a crucial EWS-FLI1 target gene in humans. Stable zebrafish transgenic lines expressing EWS-FLI1 under the control of the heat-shock promoter exhibit altered embryonic development and defective convergence and extension, suggesting that EWS-FLI1 interacts with conserved developmental pathways. These results indicate that functional targets of EWS-FLI1 that mediate tumorigenesis are conserved from zebrafish to human and provide a novel context in which to study the function of this fusion oncogene.
Development of a New Foam EOR Model From Laboratory and Field Data of the Naturally Fractured Cantarell Field
Authors: Skoreyko Fraser , Pino Antonio Villavicencio , Prada Hector Rodríguez , Nguyen Quoc P. 
Published: October 2011

Abstract
As much of the oil in the Akal field of the Cantarell complex is contained in the low permeability oil wet matrix, foam injection has been proposed as a method to control fluid mobility in the fracture, with the added benefit of transporting surfactant into the matrix so that additional oil can be liberated through a reduction of interfacial tension between oil and water. Presented in this paper is the work flow undertaken during an extensive study of all available laboratory experiments and pilot single well foam injection tests. Laboratory experiments ranged from simple water plus surfactant imbibition tests and surfactant flooding tests, to more complex foam flooding in split core experiments and co-injection of surfactant and gas for generation of foam in-situ. There were three field pilot single well foam injection tests that were included in this analysis that were of the huff-and-puff design.
This extensive analysis was done with the aid of numerical simulation that resulted in the development of a novel foam regeneration model that handles both mobility control and interfacial tension reduction effects. It is shown that with identical foam parameters, this model matches all laboratory core flood studies as well as the field pilot tests, showing that this foam model is capable of predicting foam performance in both laboratory and field settings. The foam components can be chosen to be defined as either gaseous or aqueous components and this choice is shown to affect the impact of capillary pressure on foam flow into the matrix.
Also discussed in this paper are details of how the foam behaves when injected into a gas saturated zone where the foam combines with in-situ gas, resulting in higher foam qualities than was injected. It is demonstrated that foam mobility control as a function of foam quality is an important aspect for matching field performance. The significance of correct foam density calculations is also discussed using field scale models.
The work done to match the many laboratory and field scale foam tests resulted in a significant improvement of the understanding of foam degradation, regeneration, permeability blockage, and flow in porous media and the phenomena responsible for generating incremental oil.
Diabetes Prevalence and Care in the Métis Population of Ontario, Canada
Authors: Shah Baiju R. , Cauch-Dudek Karen , Pigeau Lisa 
Published: October 2011

OBJECTIVE
The Métis are a distinct Aboriginal people in Canada with a unique history, culture, and language. This study examined diabetes prevalence and care in the Métis of Ontario.

RESEARCH DESIGN AND METHODS
The 14,480 people in the citizenship registry of the Métis Nation of Ontario were linked with provincial health care databases to determine diabetes prevalence and processes of care. Rates were compared between the Métis and the general Ontario population.

RESULTS
The age/sex standardized prevalence of diabetes for the Métis was 11.2%, nearly 25% higher than that of the general Ontario population. Métis were more likely to be hospitalized (12.7 vs. 10.7%) or require emergency room visits (36.1 vs. 27.7%).

CONCLUSIONS
Métis people have an increased burden of diabetes that puts them at risk for complications and morbidity. Ensuring adequate access to and quality of care for diabetes is essential to maintain the health of the Métis people.
Development and validation of the stability-indicating LC-UV method for the determination of cefoselis sulphate
Authors: Zalewski Przemysław , Cielecka-Piontek Judyta , Jelińska Anna 
Published: October 2011

Abstract
The stability-indicating LC assay method was developed and validated for quantitative determination of cefoselis sulphate in the presence of degradation products formed during the forced degradation studies. An isocratic, RP-HPLC method was developed with C-18 (250 × 4.6 mm, 5 µm) column and 12 mM ammonium acetate-acetonitrile (95:5 V/V) as a mobile phase. The flow rate of the mobile phase was 1.0 mL min−1. Detection wavelength was 260 nm and temperature was 30°C. Cefoselis similarly to other cephalosporins was subjected to stress conditions of degradation in aqueous solutions including hydrolysis, oxidation, photolysis and thermal degradation. The developed method was validated with regard to linearity, accuracy, precision, selectivity and robustness. The method was applied successfully for identification and determination of cefoselis sulphate in pharmaceuticals and during kinetic studies.
Parcours de vie et vieillissement ordinaire données de cadrage
Authors: Renaut Sylvie 
Published: October 2011

Vieillir est un processus de longueur et de vitesse inégales. Un vaste panorama des conditions de vie et de santé de la population générale, dès 45 ans et jusqu'à 85 ans et plus, est présenté par tranche d'âge décennal à partir de l'enquête Handicap-Santé, réalisée en 2008 par l'Insee et la Drees. La qualité du vieillissement est constitutive des différentes dimensions qui structurent le parcours de vie : l'environnement, habitat et le logement, l'organisation économique et sociale du ménage et de la famille, les ressources individuelles de santé et les modes de prise en charge.
Les seniors au festival
Authors: Djakouane Aurélien , Jourda Marie , Négrier Emmanuel , Vidal Marion 
Published: October 2011

L’objectif de cet article est d’éclairer les goûts musicaux et les pratiques culturelles d’une certaine catégorie de personnes âgées : les festivaliers. En partant d’une enquête réalisée en 2008 auprès d’une cinquantaine de festivals de musique et de danse, nous cherchons à approfondir la réflexion sur la variable de l’âge dans l’analyse des pratiques festivalières et des goûts musicaux, à partir du cas des plus de 65 ans qui représentent 20 % des festivaliers. Nous montrons qu’il n’existe pas de festivals de « seniors », tout comme il n’existe pas une palette de goûts musicaux propre à chaque génération. Cependant, celle des festivaliers âgés se distingue de celle des jeunes. Ces différences de goûts et de pratiques semblent être croissantes avec l’écart entre les populations. Plus les écarts entre les tranches d’âge s’élèvent et plus les différences de goût s’accroissent.
Vieillesses ordinaires en solitude
Authors: Campéon Arnaud 
Published: October 2011

La canicule de l’été 2003 a entraîné une prise de conscience de la vulnérabilité de nombreuses personnes âgées. Elle a permis de sensibiliser l’opinion publique à la question du lien social et à son importance au fil de l’avancée en âge. Cet article se propose d’étudier ce qui se passe lorsque ce lien fait défaut pour les personnes âgées vieillissantes. Isolées, parfois dans le dénuement le plus total, ces hommes et ces femmes peuvent en effet faire l’expérience d’une vie en solitude qu’ils ont rarement choisie et qu’ils doivent, tant bien que mal, réussir à « enchanter ». L’enquête qualitative que nous avons menée auprès d’une soixantaine de retraité(e)s apporte des renseignements sur ces solitudes « ordinaires » de la vie quotidienne.
Economical challenges to microbial producers of butanol: Feedstock, butanol ratio and titer
Authors: Gu Yang , Jiang Yu , Wu Hui , Liu Xudong , Li Zhilin , Li Jian , Xiao Han , Shen Zhaobing , Dong Hongjun , Yang Yunliu , Li Yin , Jiang Weihong , Yang Sheng 
Published: October 2011

AbstractButanol is an important solvent and transport fuel additive, and can be produced by microbial fermentation. Attempts to generate a superior microbial producer of butanol have been made through different metabolic engineering strategies. However, to date, butanol bio‐production is still not economically competitive compared to petrochemical‐derived production because of its major drawbacks, such as, high cost of the feedstocks, low butanol concentration in the fermentation broth and the co‐production of low‐value by‐products acetone and ethanol. Here we analyze the main bottlenecks in microbial butanol production and summarize relevant advances from recently reported studies. Further needs and directions for developing real industrially applicable strains in butanol production are also discussed.
Reforming of Biofuels as a Promising Option for a Sustainable Future Hydrogen Economy
Authors: Martin Stefan , Lucka Klaus , Wörner Antje , Vetter Armin 
Published: October 2011

AbstractTrotz großer technischer Fortschritte existieren noch immer zahlreiche Hindernisse für eine breite Markteinführung der Wasserstoff‐ und Brennstoffzellentechnologie. Neben der Senkung der Herstellungskosten ist hier auch der Aufbau einer Verteilungsinfrastruktur für Wasserstoff zu nennen. Entscheidend für die Beurteilung des Sekundärenergieträgers Wasserstoff ist nicht zuletzt, ob es gelingt Wasserstoff nachhaltig zu erzeugen. Unter der Annahme eines verstärkten Markteintritts von Brennstoffzellen‐Fahrzeugen ab 2020, stellt die Reformierung biogener Flüssigbrennstoffe der 1. Generation (insbesondere Pflanzenöl) eine alternative Option zur Wasserstofferzeugung dar. Die vorgestellten Potenzialabschätzungen zeigen, dass bei entsprechenden politischen Rahmenbedingungen 2020 etwa 6 – 8 % des heutigen Kraftstoffbedarfs durch nachhaltig erzeugten Wasserstoff abgedeckt werden könnte.
Tsunami: massively parallel homomorphic hashing on many‐core GPUs
Authors: Chu Xiaowen , Zhao Kaiyong , Li Zongpeng 
Published: October 2011

SUMMARYHomomorphic hash functions play a key role in securing distributed systems that use coding techniques such as erasure coding and network coding. The computational complexity of homomorphic hash functions remains a main challenge. In this paper, we present a massively parallel solution, named Tsunami, by exploiting the widely available many‐core graphic processing units (GPUs). Tsunami includes the following optimization techniques to achieve the highest ever hashing throughput: (1) using Montgomery multiplication and precomputation to speed up modular exponentiations; (2) using a clean implementation of Montgomery multiplication in order to decrease the demand of registers and shared memory and increase the utilization ratio of GPU processing cores; (3) using our own assembly code to implement the 32‐bit integer multiplication, which outperforms the assembly codes generated by the native compiler by 20%; and (4) exploiting memory alignment and constant memory on GPUs to improve the efficiency of memory access. Integrating the above techniques, our Tsunami achieves a significant improvement over existing results. Specifically, the hashing throughput achieved by Tsunami on a GTX295 GPU (NVIDIA, Santa Clara, CA, US) is about 33 times that of the existing solution on a quad‐core CPU. We also show that the hashing throughput grows almost linearly with the number of GPU cores. Copyright © 2011 John Wiley & Sons, Ltd.
Optimizing queries with expensive video predicates in cloud environment
Authors: Zhang Yansong , Yu Lisheng , Zhang Xiao , Wang Shan , Li Hui 
Published: October 2011

SUMMARYWith the rapid developments in video processing technologies, video data have increased rapidly and become popular in our daily life for both professional and consumer applications such as surveillance, education and entertainment. Because of the increasing processing workload, more and more queries with expensive video predicates are being implemented in a parallel environment for better performance. Such requirements entail that the data management system not only be able to store and access video content, but also be able to optimize queries that have expensive video predicates in an effective and efficient way in a cloud environment. In previous research literatures, parallel and distributed policies and query optimizations in relational database management systems are often based on the disk input/output (I/O) cost of involved operations and network transmission cost. However, for a query that contains expensive video predicates in a cloud environment, the traditional cost estimation model does not work well. Although researchers have proposed some approaches that can solve the problem in certain situations, there are still some unresolved issues, and these approaches need further optimizations. This paper is motivated by a real‐world large supermarket business data and video surveillance data management scenario in a parallel environment. By considering the characteristics of video data and their expensive processing, we present methods named operating results buffer and operating results buffer‐C for implementing expensive video predicates at simple node, mapping video data and executing expensive video predicates in a cloud environment, which reduce the cost of video data transmission and the invoking times of expensive video predicates. We propose a novel query optimization approach that reconstructs the join order‐based estimation for attribute cardinality and computes the total cost with I/O, network and expensive processing. This approach reduces the invoking times of expensive video predicates to a greater degree and gives a better solution for mixed query optimization, which contains traditional data types and large object operations in a cloud environment. Our query performance improves by 30% to 80% compared with existing expensive predicates query optimization methods. Copyright © 2011 John Wiley & Sons, Ltd.
Compiler supports for VLIW DSP processors with SIMD intrinsics
Authors: Kuan Chi‐Bang , Lee Jenq Kuen 
Published: October 2011

SUMMARYTo sustain growing multimedia workload, modern digital signal processing (DSP) processors are commonly equipped with subword instructions to accelerate signal processing. Besides subword, functional units of very long instruction word (VLIW) DSP processors can also be employed to process multiple data streams in parallel. However, because of power and area concerns, many embedded VLIW DSP processors adopt distributed register files to reduce read/write ports and wire connection by privatizing register files for clusters and even for functional units. The distributed design presents great challenges to compilers in distributing single instruction, multiple data (SIMD) workload to functional units. In this paper, we address the issue in supporting SIMD parallelism on VLIW DSP processors with subword instructions and distributed register files. Currently, industrial practices have adopted intrinsics that enable developers to utilize hardware resources and compete with hand‐coded assembly in performance. However, it is still an open issue to provide such a solution for VLIW DSP processors with distributed register files. In this work, we provide SIMD intrinsics to allow programmers to write highly optimized codes by following given programming guides. In addition, an enhanced register allocation scheme and data replication optimizations are devised to enable efficient code generation. In our experiments, DSPstone benchmark and a set of H.264 kernels are used to evaluate the proposed programming and optimization schemes. The result shows that by combining SIMD intrinsics and compiler optimizations, one is able to obtain remarkable performance improvements, speedups of 2.9 and 3.5 for DSPstone and H.264 kernels, respectively. Copyright © 2011 John Wiley & Sons, Ltd.
Trust assessment: a personalized, distributed, and secure approach
Authors: Carchiolo Vincenza , Longheu Alessandro , Malgeri Michele , Mangioni Giuseppe 
Published: October 2011

SUMMARYCurrently several computer‐based scenarios leverage the concept of trust as a mean to make electronic interactions (e.g., e‐commerce transactions) as reliable as possible, allowing to cope with uncertainty and risks by recommending trusted peers. Generally, the evaluation of trustworthiness can be accomplished according to many principles, from social‐based to psychology‐based; one of the commonly adopted approaches within peer‐to‐peer networks, virtual social networks, and recommendation systems is the reputation‐based trust evaluation. Because more and more large networks (even with millions of nodes) aim at leveraging trust, approaches to its assessment have to take into account the factors as efficient distributed implementation and effective security protection against malicious attacks.In this paper, we present a distributed and secure algorithm based on TrustWebRank, a metric that takes into account both personalized trust evaluation and network dynamics issues. To test our proposal both in terms of complexity and bandwidth usage, we performed simulations on a large and real dataset built from the Epinions.com recommendation system. Results show that the proposed distributed algorithm is effective and efficient, while preserving original benefits of TrustWebRank. Copyright © 2011 John Wiley & Sons, Ltd.
Optimal online deterministic algorithms and adaptive heuristics for energy and performance efficient dynamic consolidation of virtual machines in Cloud data centers
Authors: Beloglazov Anton , Buyya Rajkumar 
Published: October 2011

SUMMARYThe rapid growth in demand for computational power driven by modern service applications combined with the shift to the Cloud computing model have led to the establishment of large‐scale virtualized data centers. Such data centers consume enormous amounts of electrical energy resulting in high operating costs and carbon dioxide emissions. Dynamic consolidation of virtual machines (VMs) using live migration and switching idle nodes to the sleep mode allows Cloud providers to optimize resource usage and reduce energy consumption. However, the obligation of providing high quality of service to customers leads to the necessity in dealing with the energy‐performance trade‐off, as aggressive consolidation may lead to performance degradation. Because of the variability of workloads experienced by modern applications, the VM placement should be optimized continuously in an online manner. To understand the implications of the online nature of the problem, we conduct a competitive analysis and prove competitive ratios of optimal online deterministic algorithms for the single VM migration and dynamic VM consolidation problems. Furthermore, we propose novel adaptive heuristics for dynamic consolidation of VMs based on an analysis of historical data from the resource usage by VMs. The proposed algorithms significantly reduce energy consumption, while ensuring a high level of adherence to the service level agreement. We validate the high efficiency of the proposed algorithms by extensive simulations using real‐world workload traces from more than a thousand PlanetLab VMs. Copyright © 2011 John Wiley & Sons, Ltd.