bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fuel switching and energy partitioning during the postprandial metabolic response in the ball python (Python regius)
Authors: Waas Stefan , Werner Roland A. , Starck J. Matthias 
Published: March 2010

SUMMARY
Digestion, absorption and assimilation of the meal are active processes that require start-up energy before the energy contained in a meal can be utilized. The energetic costs associated with feeding (specific dynamic action, SDA) are high in sit-and-wait foraging snakes that tolerate long fasting periods. We used 13C-labelled prey to partition between endogenous energy sources (i.e. snakes own resources) and exogenous energy sources (i.e. prey). A linear mixing model was then applied to determine the portion of 13C originating from the different sources. The snakes showed a normal and typical postprandial response. By four hours after feeding, the δ13C-values indicated fuel switching from endogenous to exogenous. From then on, fuel mixing continuously increased until, at 20 h after feeding, 75% of fuel was exogenous. Resource partitioning showed that throughout SDA, the amount of exogenous energy increased to ~60% of SDA, which was equivalent to ~4.5% of the energy contained in a meal.
Deployment of hagfish slime thread skeins requires the transmission of mixing forcesviamucin strands
Authors: Winegard T. M. , Fudge D. S. 
Published: March 2010

SUMMARYHagfishes are benthic marine protovertebrates that secrete copious quantities of slime when threatened. The slime originates as a two-component glandular exudate comprised of coiled bundles of cytoskeletal intermediate filaments (thread skeins) and mucin vesicles. Holocrine secretion of the slime into seawater results in the rapid deployment of both fibrous and mucin components, resulting in about a liter of dilute slime. Deployment of the thread skeins involves their unraveling in a fraction of a second from a 150 μm-long ellipsoid bundle to a thread that is 100× longer. We hypothesized that thread skein deployment requires both vigorous hydrodynamic mixing and the presence of mucin vesicles, both of which are required for whole slime deployment. Here we provide evidence that mixing and mucin vesicles are indeed crucial for skein unraveling. Specifically, we show that mucin vesicles mixed into seawater swell and elongate into high-aspect ratio mucin strands that attach to the thread skeins, transmit hydrodynamic forces to them and effect their unraveling by loading them in tension. Our discovery of mucin strands in hagfish slime not only provides a mechanism for the rapid deployment of thread skeins in vivo, it also helps explain how hagfish slime is able to trap such impressive volumes of seawater via viscous entrainment. We believe that the deployment of thread skeins via their interaction with shear-elongated mucins represents a unique mechanism in biology and may lead to novel technologies for transmitting hydrodynamic forces to microscale particles that would typically be immune to such forces.
It's tough to be small: dependence of burrowing kinematics on body size
Authors: Che James , Dorgan Kelly M. 
Published: March 2010

SUMMARY
Burrowing marine infauna are morphologically diverse and range in size over several orders of magnitude. Whilst effects of ontogenetic and morphological differences on running, flying and swimming are relatively well understood, similar analyses of burrowing mechanics and kinematics are lacking. The polychaete Nereis virens Sars extends its burrow by fracture, using an eversible pharynx to exert force on the walls of the burrow. The resulting stress is amplified at the anterior tip of the burrow, which extends when the stress exceeds the fracture toughness of the material. Here we show that the polychaete Cirriformia moorei extends its burrow by a similar mechanism, but by using its hydrostatic skeleton rather than an eversible pharynx. Based on the dimensionless wedge number, which relates work of fracture to work to maintain body shape against the elasticity of sediment, we predicted that smaller worms would exhibit behaviors characteristic of tougher sediments and that scaling of kinematics would reflect decreasing difficulty in fracturing sediment with increasing body size. We found that smaller worms were relatively blunter and thicker, and had a greater variation of thickness than larger worms as they burrowed. Although these kinematic differences increase the stress amplification at the crack tip, smaller worms still generate lower stress intensity factors. The greater relative body thickness and shape changes of smaller worms are consistent with ontogenetic changes in forces exerted by earthworms, and are likely driven by the challenge of exerting enough stress to extend a crack with a small body size.
Hydrodynamic sensing does not facilitate active drag reduction in the golden shiner (Notemigonus crysoleucas)
Authors: McHenry M. J. , Michel K. B. , Stewart W. , Müller U. K. 
Published: March 2010

SUMMARYThe lateral line system detects water flow, which allows fish to orient their swimming with respect to hydrodynamic cues. However, it is unclear whether this sense plays a role in the control of propulsion. Hydrodynamic theory suggests that fish could reduce drag by coordinating the motion of the head relative to detected flow signals. To test this hypothesis, we performed measurements of undulatory kinematics during steady swimming in the golden shiner (Notemigonus crysoleucas) at three speeds (4.5, 11.0 and 22.0 cm s−1). We found that the phase shift between yaw angle and lateral velocity (20.5±13.1 deg., N=5) was significantly greater than the theoretical optimum (0 deg.) and the amplitude of these variables created a hydrodynamic index (H=0.05±0.03, N=6) that was less than an order of magnitude below the theoretical prediction. Furthermore, we repeated these measurements after pharmacologically ablating the lateral line hair cells and found that drag reduction was not adversely influenced by disabling the lateral line system. Therefore, flow sensing does not facilitate active drag reduction. However, we discovered that ablating the lateral line causes the envelope of lateral displacement to nearly double at the envelope's most narrow point for swimming at 4.5 cm s−1. Therefore, fish may use hydrodynamic sensing to modulate the lateral amplitude of slow undulatory swimming, which could allow rapid responses to changes in environmental flow.
Investigation and development of an innovative pigging technique for the water-supply industry
Authors: Quarini G , Ainslie E , Herbert M , Deans T , Ash D , Rhys D , Haskins N , Norton G , Andrews S , Smith M 
Published: March 2010

Water-supply companies monitor the state of their pipe networks and ensure that the pipes are clean and free of inert loose deposits by flushing and pigging. Flushing involves forcing high speed water through the pipes so as to carry away particulates, pigging consists of forcing an object (the pig) through the pipe so as to push or wipe away the loose material. Both systems have drawbacks; the first tends to use very large volumes of water, and it may be impossible to get the required velocities in large-diameter pipes. The second requires purpose built launch and receive stations and may run the risk of damaging the pipe walls. This paper presents an innovative alternative to water flushing or conventional pigging for the potable-water-supply industry. This alternative uses a phase change material (ice—water slurry), which can be introduced into and removed from existing pipe networks with minimal alterations. The underlying concept is that when an ice slurry is propelled through pipes at modest speeds, the wall shear is two to four orders of magnitude higher than that which would have been achieved had water (only) been travelling in the pipe at the same speed. Thus, even with relatively low speeds, the ‘ice pig’ is able to achieve efficient cleaning and removal of loose materials. This technology has the advantage that the ice pig changes its shape to fit the containing topology, hence it is able to navigate bends, contraction/expansions and partly open vales, while cleaning the containment walls and transporting particulates. Lastly, the ice pig is guaranteed never to get stuck, as it will simply melt away, if left for sufficient time. The paper presents laboratory experimental data, qualitatively demonstrating the capability of the technique and quantitative data enabling engineers to scale and size the ice pig for full scale trials. Finally, preliminary work from full scale trails on live water trunk mains is briefly presented and discussed.
Reversible Formation and Destruction of Micelles of Amphiphilic Compounds in Aqueous Media. Competition with Pseudorotaxane Formation
Authors: Suzaki Yuji , Taira Toshiaki , Osakada Kohtaro 
Published: March 2010

Abstract
N-Alkyl-4,4′-bipyridiniums [4,4′-bpy-N-(CH2)nOAr]+(Cl−) (n = 6 and 10, Ar = C6H3-3,5-t-Bu2, C6H3-3,5-(OMe)2, and C6H2-2,4,6-Me3), having the cationic bipyridinium group and a long hydrophobic alkyl chain, were prepared from the reaction of 4,4′-bipyridine with the corresponding alkyl chlorides. The amphiphilic compounds in water form micelles which encapsulate added pyrene in the hydrophobic core. Addition of α-cyclodextrin to the micellar solution converts a part of the aggregated amphiphilic molecules to their pseudorotaxane with α-cyclodextrin. Formation of the pseudorotaxanes is favored at lower temperature, as is observed by temperature dependent change of the absorption spectra.
One-Pot Synthesis and Fluorescence Properties of 2-Arylquinolines
Authors: Sueki Shunsuke , Okamoto Chiharu , Shimizu Isao , Seto Keisuke , Furukawa Yukio 
Published: March 2010

Abstract
The one-pot synthesis of 2-arylquinoline with arylamines, arylaldehyde, and 1,1-diethoxyethane were studied using a catalytic amount ytterbium triflate. Various 2-arylquinolines showed fluorescence properties and the fluorescence was quenched by introducing the nitro group into aryl moiety of 2-arylquinolines.
Crystal Structures of Two Vancomycin Complexes with Phosphate and N-Acetyl–d-Ala. Structural Comparison between Low-Affinity and High-Affinity Ligand Complexes of Vancomycin
Authors: Kikuchi Takanori , Karki Shyam , Fujisawa Ikuhide , Matsushima Yoshitaka , Nitanai Yasushi , Aoki Katsuyuki 
Published: March 2010

Abstract
Crystal structures of two vancomycin complexes with phosphate and N-acetyl–d-Ala (AcDA) were determined. Each complex involves two crystallographically independent vancomycin molecules (V1 and V2) in the asymmetric unit, which form a usually observed back-to-back arranged vancomycin dimer V1–V2 with two disaccharide chains packed in a head-to-head manner, but only one of the two ligand-binding sites is occupied. Comparison of the published crystal structures of low-affinity (small in molecular size) ligand complexes of vancomycin with high-affinity (large) ligand complexes reveals that when the high-affinity ligand binds, three structural factors (hydrogen-bonding interactions between the two peptide-backbones and hydrophobic intra-dimer sugar–ring and ring (face)–ring (edge) interactions) work to enhance the stabilization of the back-to-back dimer-interface, an important factor that is believed to promote antibacterial activity. It has also been revealed, by examining the high-affinity ligand complexes (including N-acetyl–d-Ala–d-Ala), that sugar–ligand interaction could cause different affinities of the two halves of the dimer; this is a factor responsible for the failure of the ligand binding to V1 in the AcDA complex. Possible scenarios for the formation of vancomycin complexes with low-affinity as well as high-affinity ligands are presented.
One-year outcomes of radiofrequency ablation of incompetent perforator veins using the radiofrequency stylet device
Authors: Marsh P , Price B A , Holdstock J M , Whiteley M S 
Published: March 2010

Objectives Early success treating incompetent perforator veins (IPVs) with radiofrequency ablation (RFA) and the trend to move varicose vein surgery into a walk-in walk-out service led to the design of a specific device enabling RFA of IPVs using local anaesthesia (ClosureRFS™ stylet). Our aim was to assess one-year outcomes of a clinical series of patients undergoing treatment with this device. Truncal reflux, where present, was treated initially, and RFA of IPVs was performed as a secondary procedure. Method Duplex ultrasound examinations were performed and the presence of IPVs documented. Results were compared with preoperative scans. IPVs were classified as closed, not closed/reopened or de novo. Results Of the 75 patients invited for follow-up, 53 attended at a median time of 14 months (range 11–25). Sixty-seven limbs were analysed (M:F 1:2.1, median age 62, range 25–81). Of the 124 treated IPVs, 101 were closed (82%). Clinical, aetiological, anatomical and pathological clinical score was improved in 49.3% limbs. IPV closure was reduced in patients with recurrent varicose veins compared with primary varicose veins (72.3% versus 87%, P = 0.056). Conclusion These results demonstrate the radiofrequency stylet device to be an effective treatment for IPVs.
The use of prophylaxis in patients undergoing diagnostic tests for suspected venous thromboembolism
Authors: Lee J-A , Zierler B K 
Published: March 2010

Objectives The purpose of this study was to describe the use of pharmacological and mechanical prophylaxis and clinical outcomes of patients undergoing diagnostic tests for suspected venous thromboembolism (VTE). Methods The medical records of 660 consecutive inpatients referred for suspected VTE at an academic medical centre were retrospectively reviewed. Results Acute VTE was diagnosed in 138 (21%) of the 660 patients; the incidence of deep vein thrombosis and pulmonary embolism was 18–25%, respectively. Only 61% of eligible patients received pharmacological prophylaxis and 43% of patients received mechanical prophylaxis. The incidence of VTE was higher in patients who did not receive pharmacological prophylaxis (30%) compared with patients who did (16%, P value <0.001). Conclusions Preventive measures for VTE, including both pharmacological and mechanical prophylaxis, were underutilized in hospitalized patients undergoing diagnostic tests for suspected VTE.
Anatomical failure following laparoscopic antireflux surgery (LARS); does it really matter?
Authors: Dunne N , Stratford J , Jones L , Sohampal J , Robertson R , Booth MI , Dehn TCB 
Published: March 2010

INTRODUCTION Failure rates of laparoscopic antireflux surgery (LARS) vary from 2–30%. A degree of anatomical failure is common, and the most common failure is intrathoracic wrap herniation. We have assessed anatomical integrity of the crural repair and wrap using marking Liga clips placed at the time of surgery and compared this with symptomatic outcome. PATIENTS AND METHODS A prospective study was undertaken on 50 patients who underwent LARS in a single centre over a 3-year period. Each had an X-ray on the first postoperative day and a barium swallow at 6 months at which the distance was measured between the marking Liga clips. An increase in interclip distance of > 25–49% was deemed ‘mild separation’, and an increase of > 50% ‘moderate separation’. Patients completed a standardised symptom questionnaire at 6 months. RESULTS At 6 months' postoperatively, 22% had mild separation of the crural repair with a mean Visick score of 1.18, and 54% had moderate separation with a mean Visick score of 1.26. Mild separation of the wrap occurred in 28% with a mean Visick score of 1.21 and 22% moderate separation with a mean Visick score of 1.18. Three percent had mild separation of both the crural repair and wrap with a mean Visick score of 1.0, and 16% moderate separation with a mean Visick score of 1.13. Of patients, 14% had evidence of some degree of failure on barium swallow but only one of these was significant intrathoracic migration of the wrap which was symptomatic and required re-do surgery. CONCLUSIONS The prevalence of some form of anatomical failure, as determined by an increase in the interclip distance, is high at 6 months' postoperatively following LARS. However, this does not seem to correlate with a subjective recurrence of symptoms.
Cardiopulmonary arrest on arrival due to penetrating trauma
Authors: Moriwaki Yoshihiro , Sugiyama Mitsugi , Toyoda Hiroshi , Kosuge Takayuki , Tahara Yoshio , Suzuki Noriyuki 
Published: March 2010

INTRODUCTION The aim of this study was to clarify the outcome of patients with cardiopulmonary arrest on arrival due to penetrating trauma (PT-CPA) and to establish the treatment strategy. PATIENTS AND METHODS The clinical course of 29 patients with PT-CPA over the past 10 years was examined. We have taken three approaches to these patients: (i) an aggressive treatment strategy; (ii) an in-hospital system supporting this aggressive resuscitation; and (iii) the pre-hospital emergency medical service (EMS) system in our city. RESULTS Although the return of spontaneous circulation (ROSC) was established in 59% of patients, only 17% survived for 7 days, 14% were discharged, and 7% were neurologically intact. Of 10 patients showing pulseless electrical activity (PEA) on the scene, ROSC was established in 100% and 30% were discharged; however, of 12 patients showing asystole, ROSC was established in 33% and no patient could be discharged. There was no difference in the time interval from the arrival at the emergency department to ROSC between discharged patients and patients who died. The time interval from collapse to arrival at the emergency department in discharged patients and patients who went to the intensive care unit was shorter than that of patients who died in the emergency department with and without ROSC. CONCLUSIONS We cannot decide to give up and terminate resuscitation in any PT-CPA patients and cannot define salvageable patients. However, our data show that 30-min resuscitation is thought to be relevant and that we should not give up on resuscitation because of the time interval without ROSC after arrival at the hospital.
Can the National Health Service Cancer Plan time-line be applied to colorectal hepatic metastases?
Authors: Jones Claire , Badger Stephen A , McClements Jane , McKie Lloyd , Diamond Tom , Taylor Mark A 
Published: March 2010

INTRODUCTION The National Health Service (NHS) Cancer Plan guidelines recommend a maximum 2-week wait from referral to first appointment, and 2 months from referral to treatment for primary cancers. However, there are currently no guidelines available for metastatic disease. In the UK, nearly half of all colorectal cancer patients develop hepatic metastases. Timely, surgical resection offers the potential for cure. The aim of this study was to audit current practice for colorectal liver metastases in a regional hepatobiliary unit, and compare this to the NHS Cancer Plan standards for primary disease. PATIENTS AND METHODS A retrospective review of the unit's database was performed for all hepatic metastases referrals from January 2006 to December 2008. The dates of referral, first appointment, investigations and initiation of treatment, along with patient's age and sex, were recorded on Microsoft Excel and analysed. Time was expressed as mean ± SD in days. RESULTS A total of 102 patients with hepatic metastases were identified. Five were excluded due to incomplete data. The average time from referral to first appointment was 10.6 ± 9.4 days and the average time from referral to treatment was 38.5 ± 28.6 days. Seventy-five (72.7%) had surgical intervention, of whom 37 also had chemotherapy. CONCLUSIONS The data compare favourably to the NHS Cancer Plan guidelines for primary malignancy, demonstrating that a regional hepatobiliary unit is capable of delivering a service for colorectal liver metastases that adheres to the NHS Cancer Plan. Therefore, the NHS Cancer Plan can be applied to this cohort.
Request for Treatment: the evolution of consent
Authors: Shokrollahi Kayvan 
Published: March 2010

INTRODUCTIONRequest for Treatment (RFT) is a new approach to consent which aims to facilitate patients’ understanding of their treatment and addresses some of the flaws highlighted in a literature review of current consent practice. It aims to provide a complete clinical, medicolegal, and documentary framework for consent and places patients at the centre of their care. It also provides doctors with more robust evidence that adequate consent has been obtained, and can be implemented with ease in most clinical scenarios, especially elective surgery.MATERIALS AND METHODSA thorough critical analysis and literature review is undertaken looking at the current state of consent world-wide. For the first time, a complete documentary system for ‘request for treatment’ is devised including Request for Treatment forms (RFTFs) alternatively termed Patient-centred Consent Forms (PCCFs). The arguments for the legal validity and other advantages of RFT are presented.CASE STUDYA case with all the documentation of a full consent episode is provided which illustrates RFT in action, demonstrating the simplicity of implementation, and the robustness of the completed RFT form as a source of evidence for both consent and capacity.CONCLUSIONSRequest for Treatment (RFT) is a request-based model for consent that facilitates patient-centred care. It has a number of advantages including unrivalled documentary evidence of consent in the patient&s own handwriting and vocabulary, demonstration of capacity, ease of implementation, and a sound legal basis. For those who may wish to use it, RFT provides a useful and novel patient-centred method of consent, and is likely to protect against negligent consent practice by highlighting patient misunderstandings early and by providing irrefutable documentary evidence that consent has been gained. It may also provide a simple method by which Gillick competence can be assessed and documented. RFT forms are available for download at www.rft.org.uk .
Primary Care Clinicians and the Detection and Referral of Potentially Malignant Disorders in the Mouth: A Summary of the Current Evidence
Authors: Brocklehurst Paul R , Baker Sarah R , Speight Paul M 
Published: March 2010

The importance of detecting oral malignant disease is paramount. Recent government strategies have placed an emphasis on the early detection of cancer. This paper presents a brief account of the current debate around the role of oral cancer screening and provides a summary of the results of three practice-based studies that were supported by the British Society for General Dental Surgery and the Faculty of General Dental Practice (UK). Overall, there appears to be a lack of rigour among some general dental practitioners when screening for potentially malignant disorders and their incidence in dental practice is perceived to be low. Barriers remain to the implementation of smoking-cessation schemes and many general dental practitioners feel out of their depth in tackling the abuse of alcohol, despite recognising its importance as a risk factor. Patients continue to present to their general medical practitioner (GMP) when they are concerned about something in their mouth that is not related to their teeth. GMPs’ ability to manage early oral cancer is discussed. A prospective randomised controlled trial (RCT), or a demonstration study, is recommended to investigate the numbers of cases of oral cancer diagnosed in primary dental care and primary medical care and to develop standardised referral criteria for early cases of this disease to reduce under- and over-referral. The RCT could also examine the potential of using auxiliary healthcare workers and examine the impact of such a programme upon secondary care services.