bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cognitive impairment and the role of the ApoE ε4‐allele after stroke—a 13 months follow‐up study
Authors: Wagle Jørgen , Farner Lasse , Flekkøy Kjell , Wyller Torgeir B. , Sandvik Leiv , Eiklid Kristin L. , Fure Brynjar , Stensrød Brynhild , Engedal Knut 
Published: December 2009

AbstractObjectiveTo examine the relationship between the ApoE ε4 allele and cognitive impairment 13 months after stroke.MethodsOne hundred four stroke rehabilitation patients were cognitively tested on average 18 days after hospital admission and again 13 months later with the Repeatable Battery for the Assessment of Neuropsychological Status (RBANS). The following potential risk factors for post‐stroke cognitive impairment (defined by a RBANS total index score below 77.5 points) at 13 months follow‐up were analyzed in bivariate and logistic regression analyses: ApoE‐genotype, socio‐demographic variables, pre‐stroke cognitive reduction (The Informant Questionnaire on Cognitive Decline in the Elderly (IQCODE)), vascular factors, lesion characteristics, and neurological impairment (The National Institute of Health Stroke Scale (NIHSS)). Differences in general cognitive performance (pre‐stroke, baseline, and follow‐up) across patients with different ApoE‐genotypes were analyzed, and lastly differences between ε4‐carriers and non‐carriers for changes in performance in various cognitive domains over the 13 months period were examined.ResultsSignificant risk factors for cognitive impairment at 13 months were ApoE ε4, pre‐stroke cognitive reduction (IQCODE 3.44+), previous stroke, and neurological impairment (NIHSS Total Score >5). A significant dose‐dependent effect of the ApoE‐genotype in relation to overall post‐stroke cognitive functioning was found at baseline and follow‐up, but not pre‐stroke. The ε4 carriers showed a significant decline in tests related to verbal learning and memory compared to the non‐carriers.ConclusionsThe ApoE ε4‐allele constitutes an independent risk factor for cognitive impairment at 13 months post‐stroke, and is associated with progression of cognitive decline in tasks related to verbal learning and memory. Copyright © 2009 John Wiley & Sons, Ltd.
Human papillomavirus testing and cervical cytology in primary screening for cervical cancer among women in rural China: Comparison of sensitivity, specificity, and frequency of referral
Authors: Moy Lisa M. , Zhao Fang‐Hui , Li Long‐Yu , Ma Jun‐Fei , Zhang Qing‐Ming , Chen Feng , Song Yan , Hu Shang‐Ying , Balasubramanian Akhila , Pan Qin‐Jing , Koutsky Laura , Zhang Wen‐Hua , Lim Jeanette M. , Qiao You‐Lin , Sellors John W. 
Published: December 2009

AbstractThe causal relationship between persistent high‐risk human papillomavirus infection and cervical cancer is widely accepted. HR‐HPV DNA testing, alone or in combination with Pap smear testing, may have a role in primary screening. The screening results (VIA, VILI, Pap, and HR‐HPV DNA) of 9,057 women in rural China were analyzed to determine the screening performance for the detection of CIN3+. All screening strategies had comparable AUCs (0.9). Cotesting strategies had the overall highest sensitivity for CIN3+ (99.4%), followed by HR‐HPV DNA testing alone (96.3%), Pap alone (80.2%), and reflex testing (75.4%). Reflex testing had the highest specificity (96.7%), followed by Pap alone (93.3%), HR‐HPV DNA testing alone (85.5%), and both cotesting strategies (LSIL: 84.8%, HSIL: 84.8%). Of the single‐test strategies, HR‐HPV DNA testing had a higher sensitivity (96.3% vs. 80.2%) compared with Pap testing. The specificity of the Pap test was higher (93.3% vs. 85.5%) and it had a lower percent referred for colposcopy (7.8% vs. 15.8%) than HR‐HPV DNA testing. HR‐HPV DNA testing with a 10.0 cutoff point (relative light units/cutoff ratio) had a sensitivity (85.2%) and specificity (90.6%) estimate comparable to Pap testing. A single‐test primary screening strategy with adequate performance would permit less frequent screening and be most appropriate. Of the primary screening strategies investigated in this setting in China, the performance of HR‐HPV DNA testing with an increased cutoff‐point might best meet these criteria.
Imaging of branched chain amino acid metabolism in tumors with hyperpolarized 13C ketoisocaproate
Authors: Karlsson Magnus , Jensen Pernille R. , in 't Zandt René , Gisselsson Anna , Hansson Georg , Duus Jens Ø. , Meier Sebastian , Lerche Mathilde H. 
Published: December 2009

AbstractPowerful analytical tools are vital for characterizing the complex molecular changes underlying oncogenesis and cancer treatment. This is particularly true, if information is to be collected in vivo by noninvasive approaches. In the recent past, hyperpolarized 13C magnetic resonance (MR) spectroscopy has been employed to quickly collect detailed spectral information on the chemical fate of tracer molecules in different tissues at high sensitivity. Here, we report a preclinical study showing that α‐ketoisocaproic acid (KIC) can be used to assess molecular signatures of tumors with hyperpolarized MR spectroscopy. KIC is metabolized to leucine by the enzyme branched chain amino acid transferase (BCAT), which is found upregulated in some tumors. BCAT is a putative marker for metastasis and a target of the proto‐oncogene c‐myc. Very different fluxes through the BCAT‐catalyzed reaction can be detected for murine lymphoma (EL4) and rat mammary adenocarcinoma (R3230AC) tumors in vivo. EL4 tumors show a more than 7‐fold higher hyperpolarized 13C leucine signal relative to the surrounding healthy tissue. In R3230AC tumor on the other hand branched chain amino acid metabolism is not enhanced relative to surrounding tissues. The distinct molecular signatures of branched chain amino acid metabolism in EL4 and R3230AC tumors correlate well with ex vivo assays of BCAT activity.
Targeting endothelin A receptor enhances anti‐proliferative and anti‐invasive effects of the HER2 antibody trastuzumab in HER2‐overexpressing breast cancer cells
Authors: Fischgräbe Jeanett , Götte Martin , Michels Katharina , Kiesel Ludwig , Wülfing Pia 
Published: December 2009

AbstractHuman epidermal growth factor receptor 2 (HER2) is frequently overexpressed in human breast cancers. It is known to drive tumor growth and progression and represents a prominent target in breast cancer therapy. The endothelin (ET) system, in particular ET‐1 and its receptor ETAR, is of major relevance for breast cancer growth and invasion. Having previously demonstrated coexpression of ETAR and HER2 in breast tumors, this study was designed to investigate molecular interactions of HER2 (including the epidermal growth factor receptor EGFR as its major coreceptor) and ET signaling, and the potential benefit of a combined anti‐HER2/ETAR treatment in human breast cancer cells. Dual HER2‐ETAR targeting utilizing trastuzumab (monoclonal anti‐HER2 antibody) and the ETAR antagonist atrasentan was superior to each agent alone in inhibiting basal and EGF‐induced proliferation and invasion of HER2‐overexpressing BT‐474 and SK‐BR‐3 cells. EGF‐induced invasion was partially inhibited by atrasentan alone, suggesting the involvement of ETAR in EGF receptor mediated invasion of breast cancer cells. Moreover, secretion of the pro‐invasive ET‐1 was shown to be induced by EGF via EGFR and HER2, including MAPK‐dependent signaling. In turn, an ET‐1/ETAR‐dependent regulation of EGFR protein expression and phosphorylation (at Tyr845) was observed, which may contribute to the additional anti‐proliferative and anti‐invasive effects of atrasentan on trastuzumab treated cells; reconfirming, atrasentan failed to enhance inhibitory effects of EGFR‐targeted agents. This study suggests complex interactions between HER2/EGFR and ET pathways in breast cancer and supports the hypothesis that dual HER2‐ETAR targeting may represent a highly effective approach in breast cancer treatment.
Circulating free DNA, p53 antibody and mutations of KRAS gene in endometrial cancer
Authors: Dobrzycka Bozena , Terlikowski Slawomir J. , Mazurek Andrzej , Kowalczuk Oksana , Niklinska Wieslawa , Chyczewski Lech , Kulikowski Marek 
Published: December 2009

AbstractThis study was conducted to evaluate the significance of circulating free DNA (CFDNA), p53 antibody (p53‐Ab) and mutations of KRAS gene in the development of endometrial cancer (EC). A total of 109 patients with EC (87 patients with Type I and 22 patients with Type II) took part in this study. KRAS mutations and CFDNA were detected by means of the PCR‐RFLP and enriched by the PCR‐RFPL method. ELISA was used to analyze plasma p53‐Ab. Tissue expression of P53 protein was evaluated immunohistochemically (IHC). The frequency of KRAS mutations was especially high in Grade 2 of Type I EC. CFDNA was frequently detected in patients with early stage of Type II EC at a low level of grade. It is noteworthy that the p53‐Ab positive rate increased in the higher grade of Type I tumors. A significant difference in the number of cases with the p53‐Ab was found in the advanced stage of Type I tumors. The frequency of KRAS and p53‐Ab correlates with tumor stage only in the Type I EC. Plasma CFDNA and p53‐Ab offer a chance to develop a procedure for EC Type II diagnosis. The association between tumor cells related to CFDNA and p53‐Ab with Type II tumor suggests that it might potentially serve as a marker in predicting the prognosis and offers a possibility to individualize treatment regimen.
Type‐specific human papillomavirus oncogene messenger RNA levels correlate with the severity of cervical neoplasia
Authors: Ho Chih‐Ming , Lee Bor‐Heng , Chang Shwu‐Fen , Chien Tsai‐Yen , Huang Shih‐Hung , Yan Chiu‐Cho , Cheng Wen‐Fang 
Published: December 2009

AbstractThis study aimed to evaluate whether quantitation of high‐risk human papillomavirus (HR‐HPV) E6 messenger RNA (mRNA) can be a potential biomarker for detecting the severity of cervical lesions. HPV genotyping was performed using a modified MY11/GP6+ PCR for HPV DNA amplification, followed by HPV genotype‐specific hybridization with on a gene chip. E6 type‐specific PCR was used to validate multiple infections. Quantitative real‐time reverse transcriptase (QRT‐PCR) and real‐time PCR used to measure mRNA levels and DNA viral loads of 6 HPV oncogenic types (HPV 16, 18, 31, 33, 52 and 58) in 720 liquid‐based cytology samples. The HPV DNA and RNA measurements were correlated with cervical lesions diagnosed by histopathologic examination. mRNA transcripts in the 6 types HPV DNA‐positive cases was lower in normal women and <CIN 1 (23%), women with CIN 1 (54%), CIN2+ (77%) and CIN3+ (80%) (p < 0.001). Geometric mean mRNA levels ranged from 24.5 (copies per 50 ng total RNA) in normal women and <CIN 1 to 210.8 in those with CIN 1, 629.0 in CIN2+ and 699.0 in CIN3+ (p < 0.0001). Trends of increasing viral mRNA with severity of histopathologic diagnosis were significant for HPV 16, 18, 52 and 58 transcripts but not for HPV 31 and 33 transcripts. However, geometric mean DNA viral loads of HPV 16, 18, 52 and 58 DNA did not significantly increase with the severity of cervical dysplasia. Therefore, quantitative HPV E6 mRNA levels of high‐risk HPV types are potentially useful biomarkers for distinguishing among HPV infections, cervical precancerous lesions and cancer.
Phosphatase of regenerating liver‐3 as a convergent therapeutic target for lymph node metastasis in esophageal squamous cell carcinoma
Authors: Ooki Akira , Yamashita Keishi , Kikuchi Shiro , Sakuramoto Shinichi , Katada Natsuya , Watanabe Masahiko 
Published: December 2009

AbstractPhosphatase of regenerating liver‐3 (PRL‐3) is a molecule associated with metastasis in a diverse of cancers, which, however, remains largely unknown in esophageal squamous cell carcinoma (ESCC). We examined both the clinical significance of PRL‐3 expression and its biological roles, and assessed possibilities as a therapeutic target in ESCC. PRL‐3 expression was found in 78% (69 of 88) of the primary ESCC on immunohistochemistry; it was the strong independent predictor for lymph node metastasis (LNM) on a multivariate logistic regression model (p = 0.0014, relative risk =15.20). Additionally, gene amplification was found in 3 (7.9%) of the 38 primary tumors with PRL‐3 overexpression by fluorescence in situ hybridization, but in none of the 19 tumors without it. PRL‐3 small interfering RNA robustly repressed cell proliferation, anchorage‐independent colony formation and invasion and augmented 5‐FU‐induced apoptosis in all the tested ESCC cell lines with PRL‐3 overexpression, irrespective of its gene amplification status. PRL‐3 inhibitor (1‐4‐bromo‐2‐benzylidene rhodanine) also suppressed such metastatic properties in the cell lines with PRL‐3 overexpression, but not with its low expression. Inverse effects were observed by PRL‐3 forced expression. Collectively, PRL‐3 overexpression is a frequent event associated with LNM and plays a causative role in promoting cancer progression. Moreover, the expression status may be a landmark to select patients with benefit from PRL‐3‐targeted therapy. Thus, PRL‐3 could be a convergent therapeutic target against ESCC with LNM.
Protein expression profiling of primary mammary epithelial cells derived from MMTV‐neu mice revealed that HER2/NEU‐driven changes in protein expression are functionally clustered
Authors: Park Sungwoo , Lee Kyung‐min , Ju Ji‐hyun , Kim Jaeyoon , Noh Dong‐Young , Lee Taehoon , Shin Incheol 
Published: December 2009

AbstractMMTV‐neu transgenic mice overexpressing NEU in their mammary glands develop tumor after 6 months of age. To find a novel protein biomarker using this mouse model, we identified and characterized the proteins that were differently expressed between primary mammary epithelial cells from 2 months old MMTV‐neu heterozygote mice and wild type (WT) littermates using two‐dimensional digest (ChemDigest™/Trypsin)‐LC‐MS/MS. The differentially expressed proteins were selected and analyzed using DAVID Bioinformatics resource. The proteins involved in anti‐apoptosis, purine metabolism, ribosome and proteasome functions were upregulated, whereas cell adhesion‐related proteins were downregulated in PMECs from MMTV‐neu mice when compared with WT PMECs. The results indicate that several functional units are coregulated by HER2/NEU. We hypothesize that these changes in the cellular proteome may be responsible for early onset of HER2/NEU‐driven tumorigenesis. © 2009 IUBMB IUBMB Life, 62(1):41–50, 2010
Caveolin‐1 facilitates cyclooxygenase‐2 protein degradation
Authors: Chen Shu‐Fen , Liou Jun‐Yang , Huang Tai‐Yu , Lin Yu‐Sin , Yeh Ai‐Ling , Tam Kabik , Tsai Tsung‐Huang , Wu Kenneth K. , Shyue Song‐Kun 
Published: December 2009

AbstractCyclooxygenase‐2 (COX‐2) plays major roles in diverse physiological and pathological processes such as inflammation and tumorigenesis. Transcriptional control of COX‐2 has been extensively investigated and characterized, but its post‐translational control is less clear. Here, we report a novel mechanism by which COX‐2 is degraded. Protein levels of caveolin‐1 (Cav‐1) and COX‐2 showed an inverse relation in colon cancer cell lines. COX‐2 proteins in lung and colon tissues were higher in Cav‐1 null mice than in wild‐type mice. RNAi knockdown of Cav‐1 increased COX‐2 protein level and decreased ubiquitinated COX‐2 accumulation. In addition, deletion of the carboxy (C)‐terminus of COX‐2, which contains a unique 19‐amino acid segment compared with COX‐1, resulted in reduced Cav‐1 binding and attenuated COX‐2 degradation. COX‐1 and green fluorescence protein containing the C‐terminus of COX‐2 resulted in enhanced degradation. Our findings suggest that Cav‐1 binds COX‐2 in endoplasmic reticulum (ER) and carries it for degradation via ER associated degradation. The C‐terminal region of COX‐2 is required for Cav‐1 binding and degradation. These results indicate a novel function of Cav‐1 in controlling COX‐2 expression, which may regulate physiological functions and have tumor suppression effects. J. Cell. Biochem. 109: 356–362, 2010. © 2009 Wiley‐Liss, Inc.
Purification and characterization of active caspase‐14 from human epidermis and development of the cleavage site‐directed antibody
Authors: Hibino Toshihiko , Fujita Eriko , Tsuji Yumiko , Nakanishi Jotaro , Iwaki Haruhi , Katagiri Chika , Momoi Takashi 
Published: December 2009

AbstractRestricted expression of caspase‐14 in differentiating keratinocytes suggests the involvement of caspase‐14 in terminal differentiation. We purified active caspase‐14 from human cornified cells with sequential chromatographic procedures. Specific activity increased 764‐fold with a yield of 9.1%. Purified caspase‐14 revealed the highest activity on WEHD‐methylcoumaryl‐amide (MCA), although YVAD‐MCA, another caspase‐1 substrate, was poorly hydrolyzed. The purified protein was a heterodimer with 17 and 11 kDa subunits. N‐terminal and C‐terminal analyses demonstrated that the large subunit consisted of Ser6‐Asp146 and N‐terminal of small subunit was identified as Lys153. We successfully developed an antiserum (anti‐h14D146) directed against the Asp146 cleavage site, which reacted only with active caspase‐14 but not with procaspase‐14. Furthermore we confirmed that anti‐h14D146 did not show any reactivity to the active forms of other caspases. Immunohistochemical analysis demonstrated that anti‐h14D146 staining was mostly restricted to the cornified layer and co‐localized with some of the TUNEL positive‐granular cells in the normal human epidermis. UV radiation study demonstrated that caspase‐3 was activated and co‐localized with TUNEL‐positive cells in the middle layer of human epidermis. In contrast, we could not detect caspase‐14 activation in response to UV. Our study revealed tightly regulated action of caspase‐14, in which only the terminal differentiation of keratinocytes controls its activation process. J. Cell. Biochem. 109: 487–497, 2010. © 2009 Wiley‐Liss, Inc.
IR‐spektroskopische Untersuchung von 1:1‐Addukten aus Bortrihalogeniden und Acylaziden
Authors: Fahr Egon , Neumann Lutz 
Published: December 2009

AbstractBei der Umsetzung von Acylaziden 5 mit BF3 bei ca. −60° entstehen sehr zersetzliche 1:1‐Addukte 6, die in einigen Fällen analysenrein erhalten werden konnten. Ihre IR‐spektroskopische Untersuchung zeigt, daß die Addukt‐Bildung durch Angriff des Bortrihalogenids an der CO‐Gruppierung des Acylazids erfolgt.
Acyl‐lacton‐Umlagerung, XLIV1) NMR‐spektroskopische Untersuchungen an α‐Acyl‐lactonen,‐lactamen bzw. ‐thiollactonen und Bestimmung der Keto‐Enol‐Gleichgewichte
Authors: Wamhoff Heinrich , Lander Helmut , Korte Friedhelm 
Published: December 2009

AbstractAus den NMR‐Spektren von 22 α‐Acyl‐lactonen, ‐lactamen und ‐thiollactonen in Deuterochloroform und Hexadeutero‐dimethylsulfoxid werden die jeweiligen Keto‐Enol‐Gleichgewichte in Abhängigkeit von Acyl‐Rest und Substitution bestimmt. Sie stimmen in nahezu allen Fällen mit den durch Enol‐Titration gewonnenen Werten überein.
Über die Struktur der Thioamide und ihrer Derivate, II1) Untersuchungen an N‐[β‐Hydroxy‐äthyl]‐N‐alkyl‐thioformamiden. Trennung von geometrischen Isomeren und NMR‐spektroskopische Konfigurationszuordnungen
Authors: Walter Wolfgang , Maerten Gerhard 
Published: December 2009

AbstractN[β‐Hydroxy‐äthyl‐]N‐alkyl‐thioformamide mit den Resten Methyl (1), Äthyl (2) und Isopropyl (3) werden durch präparative Schichtchromatographie in geometrische Isomere (a bzw. b) getrennt und deren Konfigurationen durch NMR‐Spektroskopie bestimmt. In den Gleichgewichten überwiegt die Konfiguration, in welcher der größere Substituent dem Schwefel gegenübersteht. Die N‐tert.‐Butyl‐Verbindung 4 ist chromatographisch einheitlich. An der N‐Äthyl‐Verbindung 2 werden die Enthalpie‐Differenz der Isomeren (ΔH = 610 ± 150 cal/mol) und bei 60° die freie Aktivierungsenthalpie der behinderten Rotation (25.2 ± 0.5 kcal/Mol) bestimmt.
Lewissäure‐katalysierte Umsetzung von aliphatischen Ketonen mit tert.‐Butyl‐isonitril
Authors: Zeeh Bernd , Müller Eugen 
Published: December 2009

AbstractAus Aceton bzw. Methyl‐äthyl‐keton und tert.‐Butyl‐isonitril bilden sich in Anwesenheit von Bortrifluorid Alken‐(1)‐yl‐(2)‐glyoxylsäure‐tert.‐butylamide (2 bzw. 3) sowie α‐Hydroxycarbonsäure‐tert.‐butylamide (7 bzw. 8).
Selen‐spirane
Authors: Hellwinkel Dieter , Fahrbach Gerhard 
Published: December 2009

AbstractBei der Umsetzung von 2.2′‐Biphenylylen‐selenid‐tosylimin (7a) mit 2.2′‐Dilithium‐biphenyl (8) entsteht Bis‐2.′‐biphenylylen‐selen (4a), das mit Wasser bzw. Brom unter Öffnung eines Ringes zu den Selenoniumsalzen 9 bzw. 10 reagiert. Analog erhält man Bis‐4.4′‐dimethyl‐2.2′‐biphenylylen‐selen (4b), das, wie u. a. aus massenspektroskopischen Untersuchungen folgt, wesentlich instabiler als die Stammverbindung 4a, ist.
Synthesen mit substituierten Malondialdehyden, IV1) γ‐Nitro‐tetramethinmerocyanin‐ und γ‐Nitro‐pentamethincyanin‐Farbstoffe2)
Authors: Reichardt Christian 
Published: December 2009

AbstractDurch Kondensation von Natrium‐nitromalondialdehyd (2) mit heterocyclischen Immoniumsalzen 1 werden neben Tetramethinmerocyanin‐Farbstoffen 3 symmetrische und unsymmetrische Pentamethincyanin‐Farbstoffe 4 dargestellt, deren Polymethin‐Kette durch eine Nitrogruppe substituiert ist. Diese Farbstoffe eignen sich zum Studium der Beziehung zwischen der Elektronegativität eines Substituenten und seinem Einfluß auf das UV‐Absorptionsspektrum (Abb. 4).
Synthese von Azastilbenen
Authors: Zymalkowski Felix , Reimann Eberhard 
Published: December 2009

AbstractZur Synthese N‐haltiger Analoga des Stilböstrols werden aus 4‐Acyl‐pyridinen (4, 9, 12) mit Lithium‐organischen Verbindungen die entsprechenden tertiären Alkohole (10, 13) gewonnen. Diese liefern durch Wasser‐Abspaltung bei tiefer Temperatur Azastilbene (2, 14) als cis/trans‐Isomeren‐Gemische. Ihre Stilben‐Struktur wird durch das NMR‐Spektrum bewiesen.
Über ein Imidazolium‐Analogon des Thiamins mit antagonistischer Wirkung
Authors: Staab Heinz A. , Schwalbach Gerd A. 
Published: December 2009

AbstractEine Thiamin‐analoge Verbindung (2), in der der Thiazolium‐Teil des Thiamins durch ein entsprechend substituierte N‐Methyl‐imidazolium‐Gruppe ersetzt ist, wird dargestellt und erweist sich bei Lactobacillus fermenti als Thiamin‐Antagonist.
Synthesen in der Reihe der Indole und Indolalkaloide, VII1) Synthese eines undecacyclischen Di‐indolin‐diäthers vom Typ dimerer Calebassencurare‐Alkaloide
Authors: Fritz Helmut , Rubach Günther 
Published: December 2009

AbstractDie Synthese des diquartären Ammoniumsalzes 4 wird beschrieben. Diese undecacyclische Verbindung hat die wesentlichen Konstitutionsmerkmale dimerer Calebassencurare‐Alkaloide, die in ihrem zentralen Ringsystem auf der Oxydationsstufe von C‐Curarin‐I stehen. – Im Carbazolenin 9 und der dimeren Verbindung 19 sind zwei Urethane neuen Typs aufgefunden worden, in deren NMR‐Spektren die O‐Äthyl‐Signale eine temperaturabhängige Aufspaltung zeigen.
Ubichinone und verwandte Substanzen, XII1) Umsetzung von Acetyl‐ubichromenol(6) mit Lithiumalanat
Authors: Morimoto Hiroshi , Imada Isuke , Watanabe Masazumi , Sugihara Hirosada 
Published: December 2009

AbstractAus Acetyl‐ubichromenol(6) (3) werden mit LiAlH4 γ‐Hydroxy‐isoubichinon (7) (4, γ‐Hydroxy‐β.γ‐dihydro‐ubichinon(7) (5), Ubichinon(7) (6), Desmethyl‐ubichinon(7) (7; 2‐Methoxy‐3‐hydroxy‐5‐wird dadurch nachgewiesen, daß man Ubichromenol(6) (2) mit Natrium in Äthanol zu Ubichromanol(6) (8) reduziert und dieses oxydativ zu 5 aufspaltet. Aus den NMR‐Spektren inst zu schließen, daß 6 und 7 aus einem Gemisch der cis‐trans‐Isomeren an der Δβ‐Doppelbindung in der Isopren‐Seitenkette bestehen. Die Mechanismen dieser Reaktionen werden mit Hilfe von LiAlD4 (statt LiAlH4) und anhand der Massenspektren studiert.