bluuebunny/crossref_metadata_2025_split huggingface dataset:
RNA polymerase II transcriptional silencing in growing and fully grown germinal vesicle oocytes isolated from gonadotropin‐stimulated and non‐stimulated gilts
Authors: Barnetova Irena , Morovic Martin , Strejcek Frantisek , Østrup Olga , Hyttel Poul , Niemann Heiner , Laurincik Jozef , Fulka Josef , Fulka Helena 
Published: July 2012

AbstractGlobal transcription silencing occurs in the oocyte during its final phase of growth. The particular mechanism of this silencing is not well understood. Here, we investigated the silencing of RNA polymerase II transcription in porcine oocytes. First, we investigated the transcriptional activity of germinal vesicle oocytes derived from stimulated and non‐stimulated gilts, but no transcriptional activity was observed. Second, we focused on the fate of RNA polymerase II in growing and fully grown oocytes. Active and inactive forms of RNA polymerase II were detected in growing oocytes by immunofluorescence and Western blots. In contrast, only the inactive form of RNA polymerase II was detected in fully grown oocytes. To evaluate if the inactive form of RNA polymerase II is released from DNA, the oocytes were subsequently permeabilized and fixed in one step. After this modified fixation protocol, the immunofluorescent labeling was negative in fully grown oocytes, but remained unchanged (positive) in growing oocytes. These results indicate that the inactive form of RNA polymerase II is not bound to DNA during the oocyte growth. Finally, based on Western blot analysis of different stages of oocyte maturation, the inactive form of RNA polymerase II was detected in metaphase I but not in metaphase II. Our study confirmed the global transcription silencing of fully grown oocytes. Compared with other mammalian species (e.g., mouse), the mechanism of RNA polymerase II silencing in porcine oocytes seems to be similar, despite some differences in dynamics. Mol. Reprod. Dev. 79: 697–708, 2012. © 2012 Wiley Periodicals, Inc.
A multi‐professional UK wide survey of undergraduate continence education
Authors: McClurg D. , Cheater F.M. , Eustice S. , Burke J. , Jamieson K. , Hagen S. 
Published: July 2012

AbstractObjectivesFindings from national audits and enquiries continue to report that care for patients with continence problems is often substandard and inadequate education is often cited as one of the probable causes. These factors combined with the forecasted increase in the number of people with incontinence prompted us to undertake a survey of all UK Higher Education Institutes (HEIs) to establish the amount of undergraduate continence education within relevant healthcare programs—medical, adult nursing, mental health nursing, learning disabilities nursing, children's nursing, midwifery, physiotherapy, and occupational therapy.DesignAn on line questionnaire targeted course program leads (n = 362) in all 86 HEIs in which undergraduate professional healthcare programs were provided (n = 362).Main Outcome MeasureEighty‐six HEIs were approached, 85 agreed to participate in the survey. A response rate of 81% (n = 294/362 programs) was obtained: 14% (n = 42) of respondents reported that there was no continence‐related education within their undergraduate program. The mean number of hours was 4.7 (SD 4.3), and ranged from 2.5 (SD 3.5) hr (Occupational Therapy) to 7.3 (SD 4.8) hr (Adult Nursing).ConclusionThe survey results indicate that the amount of undergraduate education has changed little. Further research is needed to identify the most appropriate methods of delivering continence education and translate knowledge into improved patient outcomes. Adequate undergraduate continence education directed by the General Medical Council, Royal Colleges and Health Professional Council is required. Neurourol. Urodynam. 32: 224–229, 2013. © 2012 Wiley Periodicals, Inc.
Battery explantation after sacral neuromodulation in the medicare population
Authors: Cameron Anne P. , Anger Jennifer T. , Madison Rodger , Saigal Christopher S. , Clemens J. Quentin ,   the Urologic Diseases in America Project
Published: July 2012

AbstractAimsTo determine sacral neuromodulation battery life and the patient and provider risk factors for early explantation in a population‐based sample.MethodsA 5% sample of Medicare beneficiaries from 1997 to 2007 served as the data source. All patients who had a sacral neuromodulation device implanted in that time period were included. Variables included in a multivariate analysis of risk factors for removal included gender, age, race, diagnosis, type of test phase, provider specialty, and volume. The number of device reprogramming events was also recorded in this time period.ResultsMean follow‐up was 60.5 months. Patients on average had 2.15 reprogramming episodes in their first year, with that number decreasing over subsequent years. Out of the 558 batteries implanted 63 (11.3%) were explanted. Of the 19 implanted individuals who carried the diagnosis of interstitial cystitis (IC), 11 (57.9%) had the battery removed. This was the only variable that predicted early removal, with an odds ratio of explantation of 10.5 (95% CI: 3.9–28.4).ConclusionVery few sacral neuromodulation batteries, once implanted, are removed prematurely. Patients with IC, however, are at very high risk of requiring premature battery removal. Neurourol. Urodynam. 32: 238–241, 2013. © 2012 Wiley Periodicals, Inc.
A phase I trial and pharmacokinetic study of a 24‐hour infusion of trabectedin (Yondelis®, ET‐743) in children and adolescents with relapsed or refractory solid tumors
Authors: Chuk Meredith K. , Aikin Alberta , Whitcomb Trish , Widemann Brigitte C. , Zannikos Peter , Bayever Eliel , Balis Frank M. , Fox Elizabeth 
Published: July 2012

AbstractBackgroundThe objectives of this phase I study were to determine the maximum tolerated dose (MTD), toxicity profile, and pharmacokinetics of a 24‐hour continuous intravenous infusion of trabectedin administered to children and adolescents with refractory or relapsed solid tumors.ProcedurePatients between the ages of 4 and 16 years old with refractory solid tumors received trabectedin as a 24‐hour infusion every 21 days. Dexamethasone and prophylactic growth factor support were administered with each cycle. Pharmacokinetic studies were conducted during cycle 1.ResultsPatients (n = 12) median (range) age 14.5 (8–16) years received trabectedin at 1.1 (n = 3), 1.5 (n = 6), or 1.7 (n = 3) mg/m2. At the 1.5 mg/m2 dose level, one patient had dose limiting anorexia and fatigue. At 1.7 mg/m2, two patients experienced dose limiting toxicity, dehydration, and gamma‐glutamyl transpeptidase elevation. Non‐dose limiting toxicities included elevated serum transaminases, myelosuppression, nausea, emesis, and fatigue. Plasma pharmacokinetic parameters were similar to historical data in adults. One partial response was observed in a patient with neuroendocrine carcinoma. Stable disease (≥6 cycles) was achieved in three patients (osteosarcoma n = 2, desmoplastic small round cell tumor n = 1).ConclusionsThe MTD of trabectedin in pediatric patients with refractory solid tumors is 1.5 mg/m2 IV over 24 hours every 21 days. Dexamethasone to ameliorate hepatic toxicity and prophylactic growth factor support are required. Pediatr Blood Cancer 2012; 59: 865–869. © 2012 Wiley Periodicals, Inc.
Spatially correlated environmental factors drive synchronisation in populations of the Dalmatian Pelican
Authors: Doxa Aggeliki , Theodorou Konstantinos , Crivelli Alain J. , Hatzilacou Dionyssia , Catsadorakis Giorgos , Malakou Myrcini , Nazirides Theodoros , Robert Alexandre 
Published: July 2012

AbstractSpatial synchrony in population dynamics has been documented recently across a range of taxa, and a number of hypotheses about the mechanisms driving spatial synchrony and the consequences of this phenomenon for the persistence of populations have emerged. Spatial environmental covariance is one of the principal factors influencing this synchrony on a large scale. However, most studies focus on population abundances, and little evidence exists on the spatial synchrony of demographic parameters. We used a 15‐year dataset from two populations of a vulnerable bird species, the Dalmatian Pelican (Pelecanus crispus), to identify local and global environmental factors that cause population synchrony. We show that survival rates were temporally synchronised between the studied populations and that a large part (>50 % for both populations) of this covariation was driven by local environmental conditions. Several components of the North Atlantic Oscillation index were correlated with local climatic conditions, but not all of these components can be used as informative proxies for future variation in survival. We also present evidence that an individual's future survival can be strongly influenced by the conditions occurring during the early period of its life. Environmental factors such as water level and food availability had similar influences on breeding success and juvenile survival. Juvenile survival was lower during dry years and years of low food availability. This finding indicated that intra‐specific competition may act as a limiting factor for species demography, especially in large populations. Estimating the strength of synchrony is important and should be considered in population and metapopulation analyses and in relationship to conservation measures.
Development of in vitro gene delivery system using ORMOSIL nanoparticle: Analysis of p53 gene expression in cultured breast cancer cell (MCF-7)
Authors: Rejeeth Chandrababu , Kannan Soundarapandian , Muthuchelian Krishnasamy 
Published: July 2012

Abstract
This article reports on the application of organically modified silica (ORMOSIL) nanoparticles as an efficient in vitro gene delivery system in the recent years. Based on that prime objective, the present study addresses the possible ways to reduce cancers incidence at cellular level. In this context, ORMOSIL nanoparticles had been synthesized and incubated along with pCMV–Myc (3.8 kb) plasmid vector construct carrying p53gene, and transfected into the breast cancer cell line MCF-7 cells. Western blot analysis showed that the p53 protein was significantly expressed in breast cancer cell upon transfection. The confocal and electron microscopic studies further confirmed that the nanoparticles were accumulated in the cytoplasm and the nucleus of the cancer cells transfected with p53 gene. Interesting agarose gel electrophoresis studies revealed that the nanoparticles efficiently complex with pCMV–Myc vector. The anti-cancer properties of p53 were demonstrated by assessing the cell survival and growth rate which showed a positive linear correlation in cancer cells. Whereas, the growth rate was significantly reduced in ORMOSIL/p53/pCMV–Myc transfected breast cancer cells compared to the growth rate of non-transfected cells. The results of this approach using ORMOSIL nanoparticles as a non-viral gene delivery platform have a promising future for use as effective transfection agent for therapeutic manipulation of cancer cells and targeted cancer gene therapy in vivo.
Classification and counter-classification of language on Saint Barthélemy
Authors: Pressman Jon F. 
Published: July 2012

ABSTRACTThis article analyzes the use of metapragmatic description in the ethnoclassification of language by native speakers on the Franco-Antillean island of Saint Barthélemy. A prevalent technique for metapragmatic description is facilitated by the differential formation of honorific registers in the island's indigenous languages, so that speakers essentialize honorific pronouns as tropes of whole languages and classify the languages in such terms. This process reflects the varied geolinguistic and generational attributes of these speakers, whose register or social-dialectal calculations are all based ultimately on folk ideologies of honorification. (Classification, language ideology, French Creole, metapragmatics, Caribbean sociolinguistics)
Oceanography and inshore hydrography of the Inner Hebrides
Authors: Ellett D. J. , Edwards A. 
Published: July 2012

SynopsisThe chief physical processes encountered in Inner Hebridean waters are described, including the tidal and current regimes, temperature and salinity variations, and stratification in the sea and sea-lochs. Although information on some of these topics is sparse, available data are used to provide order of magnitude estimates for the volume transport of the coastal current and run-off water and to indicate expected ranges of the major parameters of the marine environment. The islands are shown to be important as a region of tidal mixing of coastal water.
Libman–Sacks endocarditis as the first manifestation of systemic lupus erythematosus in an adolescent, with a review of the literature
Authors: Sharma Jayendra , Lasic Zoran , Bornstein Abraham , Cooper Rubin , Chen Jonathan 
Published: July 2012

AbstractLibman–Sacks endocarditis is rare in children and adolescents, more so as a first manifestation of systemic lupus erythematosus. Currently, sterile verrucous lesions of Libman–Sacks endocarditis are recognised as a cardiac manifestation of both systemic lupus erythematosus and antiphospholipid syndrome. They are clinically silent in a majority of the cases. The presence of antiphospholipid antibodies in systemic lupus erythematosus is associated with three times higher prevalence of mitral valve nodules and significant mitral regurgitation. We present the case of isolated mitral regurgitation with abnormal looking mitral valve, detected in early childhood, which deteriorated to a severe degree in the next decade and was diagnosed as Libman–Sacks endocarditis after surgical repair from histopathology. The full-blown clinical spectrum of systemic lupus erythematosus with antiphospholipid antibodies was observed several weeks after cardiac surgery. We discuss the atypical course of Libman–Sacks endocarditis with follow-up for 10 years, along with a review of the literature.
Economic Values for Saltwater Sport Fishing in Alaska: A Stated Preference Analysis
Authors: Lew Daniel K. , Larson Douglas M. 
Published: July 2012

Abstract
The knowledge of how anglers value their fishing opportunities is a fundamental building block of a sound marine policy, especially for stocks for which there is conflict over allocation between different uses (e.g., allocation between recreational and commercial uses and conservation goals). This paper reports on how recreational saltwater anglers value their catches, and the regulations governing them, of Pacific halibut Hippoglossus stenolepis, Chinook salmon Oncorhynchus tshawytscha, and coho salmon O. kisutch off the coast of Alaska using stated preference choice experiment data from 2007. Using data from a stated preference survey, we estimated the economic value, or willingness to pay, anglers place on saltwater boat fishing trips in Alaska and assess their response to changes in the characteristics of fishing trips. The results show that Alaska resident anglers had mean trip values ranging from US$246 to $444, while nonresidents had much higher values ($2,007 to $2,639), likely reflecting the fact that their trips are both less common and considerably more expensive to take. Nonresidents generally had significant positive values for increases in the number of fish caught, bag limit, and fish size, while Alaska residents valued size and bag limit changes but not catch increases. The economic values are also discussed in the context of allocation issues, particularly as they relate to the sport fishing and commercial fishing sectors for Pacific halibut, which is a current issue facing Alaska marine fisheries managers.
Received September 8, 2011; accepted March 22, 2012
Mayo Clinic Atlas of Regional Anesthesia and Ultrasound-Guided Nerve Blockade
Authors: Hebl James , Lennon Robert 
Published: July 2012

Abstract
Mayo Clinic Atlas of Regional Anesthesia and Ultrasound-Guided Nerve Blockade is a practical guide for residents-in-training and clinicians to gain greater familiarity with regional anesthesia and acute pain management to the upper and lower extremity. It emphasizes the importance of a detailed knowledge of applied anatomy to safely and successfully performing regional anesthesia. It also provides and overview of the emerging field of ultrasound-guided regional anesthesia, which allows reliable identification of both normal and variant anatomy. Mayo Clinic Atlas of Regional Anesthesia and Ultrasound-Guided Nerve Blockade contains more than 200 beautifully illustrated anatomic images important to understanding and performing regional anesthesia. Corresponding ultrasound images are provided when applicable.
PIN Auxin Efflux Carriers Are Necessary for Pulse-Induced But Not Continuous Light-Induced Phototropism in Arabidopsis
Authors: Haga Ken , Sakai Tatsuya 
Published: July 2012

Abstract
Auxin efflux carrier PIN-FORMED (PIN) proteins are thought to have central roles in regulating asymmetrical auxin translocation during tropic responses, including gravitropism and phototropism, in plants. Although PIN3 is known to be involved in phototropism in Arabidopsis (Arabidopsis thaliana), no severe defects of phototropism in any of the pin mutants have been reported. We show here that the pulse-induced, first positive phototropism is impaired partially in pin1, pin3, and pin7 single mutants, and severely in triple mutants. In contrast, such impairment was not observed in continuous-light-induced second positive phototropism. Analysis with an auxin-reporter gene demonstrated that PIN3-mediated auxin gradients participate in pulse-induced phototropism but not in continuous-light-induced phototropism. Similar functional separation was also applicable to PINOID, a regulator of PIN localization. Our results strongly suggest the existence of functionally distinct mechanisms i.e. a PIN-dependent mechanism in which transient stimulation is sufficient to induce phototropism, and a PIN-independent mechanism that requires continuous stimulation and does not operate in the former phototropism process. Although a previous study has proposed that blue-light photoreceptors, the phototropins, control PIN localization through the transcriptional down-regulation of PINOID, we could not detect this blue-light-dependent down-regulation event, suggesting that other as yet unknown mechanisms are involved in phototropin-mediated phototropic responses.
Challenges in implementing a functional ISO 14001 environmental management system
Authors: Searcy Cory , Morali Oguz , Karapetrovic Stanislav , Wichuk Kristine , McCartney Daryl , McLeod Sheldon , Fraser Don 
Published: July 2012

PurposeThe purpose of this paper is to present the key findings of a colloquium on ISO 14001. The colloquium focused on three key areas: critical loops and synergies among management system elements, the full spectrum of management system auditing, and integrating management systems.Design/methodology/approachA total of 40 Canadian experts on environmental management systems from a wide variety of sectors participated in the colloquium.FindingsThe colloquium provided insights into implementation issues around the three key areas noted above. The colloquium focused on discussing issues associated with corrective and preventive action, objectives and targets, internal and external auditing, and integrating change into management systems. The issues are presented in detail in the paper.Originality/valueThe participants in the colloquium believed that the findings will be of interest to both practitioners and academics in environmental management systems. The findings will also be of interest to practitioners and academics in quality and other standardized management systems.
Exploring the role of anticipated guilt on pro‐environmental behavior – a suggested typology of residents in France based on their recycling patterns
Authors: Elgaaied Leila 
Published: July 2012

PurposeSo far, few studies dealing with the determinants of pro‐environmental behavior have examined the impact of emotional variables. This research aims to extend previous work on the role of affective motivations underlying ecological behavior by exploring the influence of anticipated guilt on recycling.Design/methodology/approachA cluster analysis was conducted among 276 French interviewees based on their current recycling behavior, anticipated guilt, environmental concern, awareness of negative consequences and beliefs about facilitating conditions in their community of residence. In order to confirm assumptions related to the suggested typology, further quantitative tests were performed.FindingsThree profiles were identified. Results suggest that environmental concern and awareness of the negative consequences associated with the increase of waste volume are not sufficient conditions to stimulate diligent recycling efforts. Anticipated guilt appears to influence behavior more directly and totally mediates the relationship between environmental concern and intention to recycle.Research limitations/implicationsImplications of this research exclude countries where recycling is mandatory or represents a strongly internalized social norm.Practical implicationsThis study holds important implications in terms of public authorities' intervention. The emergence of anticipated guilt as a key determinant of intention to recycle suggests that guilt appeals could be a relevant communication strategy in order to promote recycling.Originality/valueThis research provides new insights to understand the role of anticipated guilt on ecological behavior using a typology. A predictive model of intention to recycle was also proposed.
The relationship between employee satisfaction and customer satisfaction
Authors: Jeon Hoseong , Choi Beomjoon 
Published: July 2012

PurposeThis study aims to examine whether the relationship between employee satisfaction (ES) and customer satisfaction (CS) is bilateral or unilateral based on dyadic data. In addition, it seeks to examine the role of moderating variables which have incremental impacts on this link.Design/methodology/approachThe authors conducted an empirical test on this relationship in an educational service context. Structural equation modeling was employed to test the hypotheses.FindingsResults indicate that employee satisfaction leads to CS but CS did not affect ES, which suggests that the relationship between ES and CS is unilateral rather than bilateral. The findings also demonstrate that the dispositional variables (i.e. self efficacy, cooperative orientation) moderate the impact of ES on CS.Research limitations/implicationsThis study provided theoretical implications for the ES‐CS relationship.Practical implicationsThis finding suggests that top level management in the service industry must take an active role in recruiting employees who are confident in their abilities and who display pro‐social dispositions.Originality/valueThe linkage between ES and CS has been previously examined but the findings have been inconsistent. The authors used dyadic data to investigate the relationship between ES and CS and found the influence of ES on CS but not vice versa.