bluuebunny/crossref_metadata_2025_split huggingface dataset:
Histomorphologic and histomorphometric evaluation of various endosseous implant healing chamber configurations at early implantation times: a study in dogs
Authors: Marin Charles , Granato Rodrigo , Suzuki Marcelo , Gil Jose N. , Janal Malvin N. , Coelho Paulo G. 
Published: January 2010

AbstractAim: The objective of this study was to evaluate the early healing of endosseous implants presenting various healing chamber configurations in a beagle dog mandible model.Methods: The four premolars of 12 beagle dogs were extracted and allowed to heal for a period of 8 weeks. Implants allowing six different healing chamber configurations were placed in each dog (three per side, six configurations per dog). The animals were sacrificed after 3 and 5 weeks in vivo (n=6 per time in vivo), and the implants were non‐decalcified processed to slides of ∼30 μm thickness. Bone‐to‐implant contact (BIC) and bone area fraction occupied (BAFO) within the healing chamber were quantified. Statistical analysis was performed by a GLM ANOVA model at 5% significance level.Results: Osseointegration and healing with woven bone filling throughout all healing chambers was observed. Replacement of woven bone by lamellar bone showing primary osteonic structures was observed at 5 weeks. BIC was significantly affected by healing chamber configuration (P<0.001) and was not affected by time in vivo (P>0.42) at 3 and 5 weeks in vivo. BAFO was not affected by healing chamber configuration (P>0.14) however significantly increased over implantation time (P<0.001).Conclusion: Regardless of healing chamber design and dimensions considered, healing allowed the devices osseointegration. However, healing chamber configuration significantly affected osseointegration measurable parameters such as BIC. To cite this article:
Marin C, Granato R, Suzuki M, Gil JN, Janal, MN Coelho PG. Histomorhpologic and histomorphometric evaluation of various endosseous implant healing chamber configurations at early implantation times: a study in dogs. Clin. Oral Impl. Res. 21, 2010; 577–583. doi: 10.1111/j.1600‐0501.2009.01853.x
Biological properties of a biomimetic membrane for guided tissue regeneration: a study in rat calvarial defects
Authors: Zhang Jingchao , Huang Cui , Xu Qian , Mo Anchun , Li Jidong , Zuo Yi 
Published: January 2010

AbstractObjective: The biological properties of nano‐hydroxyapatite/polyamide (nHA/PA66) membrane as a guided tissue regeneration barrier were investigated based on a critical‐size calvarial defect model.Material and methods: Thirty‐six Sprague–Dawley rats were divided into four groups: nHA/PA66 membranes, control treated with expanded polytetrafluoroethylene (e‐PTFE) membranes, control treated with PA membranes and blank. Bone regeneration in each group was evaluated using morphological, serological, radiographic and histological techniques at 1, 4 and 8 weeks, respectively, post‐operation.Results: Slight degradation of nHA/PA66 membranes was observed by a scanning electron microscope (SEM) at 4 and 8 weeks. The serum alkaline phosphatase level and the integral optical density of neo‐bone were significantly higher in the nHA/PA66 group than those in the PA group and blank (P<0.05). Radiographic and histological analysis showed a fully closed cranial defect for both the nHA/PA66 group and the e‐PTFE group at 8 weeks. No remarkable difference was found between the nHA/PA66 group and the e‐PTFE group at each time interval.Conclusion: nHA/PA66 membrane promoted bone regeneration as effectively as conventional e‐PTFE membrane. Based on a rat study, nHA/PA66 membrane provides a promising alternative to e‐PTFE membrane due to the absence of requirement for a second surgery. To cite this article:
Zhang JC, Huang C, Xu Q, Mo AC, Li JD, Zuo Y. Biological properties of a biomimetic membrane for guided tissue regeneration: a study in rat calvarial defects. Clin. Oral Impl. Res. 21, 2010; 392–397. doi: 10.1111/j.1600‐0501.2009.01857.x
The impact of dietary advice on edentulous adults' denture satisfaction and oral health‐related quality of life 6 months after intervention
Authors: Ellis Janice S. , Elfeky Ahmed F. , Moynihan Paula J. , Seal Chris , Hyland Robert M. , Thomason Mark 
Published: January 2010

AbstractObjective: The aim of this study was to compare the impact of customised dietary advice on patients' satisfaction with their dentures and oral health‐related quality of life (OHRQoL) in patients wearing implant‐supported mandibular overdentures (IOD) or conventional dentures (CD).Materials and methods: In this prospective cohort study, 28 IOD (two implant‐retained mandibular overdenture) and 26 CD patients completed a denture satisfaction scale and the 20‐item oral health impact profile (OHIP‐20) before and 6 months following provision of customised dietary advice.Results: At 6 months following provision of individualised dietary advice, the IOD group showed significantly greater satisfaction than the conventional group for denture comfort (80.6, ± 15.6, vs. 68.7 ± 15.6 P=0.001), stability (75.8 ± 15.9 vs. 59.5 ± 30.4, P=0.002), and perceived chewing ability (79 ± 30.4 vs. 59.5 ± 30.4, P=0.027) ± when adjusted for baseline scores. Before delivery of the dietary advice there were no significant differences between groups for Satisfaction or OHIP scores. No difference in OHRQoL was seen between groups.Conclusions: The delivery of customised dietary advice to edentulous patients impacts differently on their satisfaction with denture comfort, stability and chewing ability depending on the nature of their prosthesis. This re‐evaluation of satisfaction occurs when edentulous patients challenge themselves to consume more fruits, vegetables and fibre‐rich foods. The IOD group reported an increased level of satisfaction and perceived chewing ability whereas it appeared that CD wearers may have had their awareness of the shortcomings of this sort of prosthesis reawakened. To cite this article:
Ellis JS, Elfeky AF, Moynihan PJ, Seal C, Hyland RM, Thomason M. The impact of dietary advice on edentulous adults' denture satisfaction and oral health‐related quality of life 6 months after intervention. Clin. Oral Impl. Res. 21, 2010; 386–391. doi: 10.1111/j.1600‐0501.2009.01859.x
Severe idiopathic erythroblastic synartesis: successful treatment with the anti‐CD20 monoclonal antibody rituximab
Authors: Papakonstantinou Georgios , Loeffler Helmut , Haferlach Torsten , Brugger Wolfram 
Published: January 2010

AbstractErythroblastic synartesis is a very rare disorder, considered to be caused by autoimmune mechanisms, leading to aggregation of erythroid precursor cells in the bone marrow and subsequently to acquired dyserythropoiesis with severe, transfusion‐dependent anemia. An association with lymphoproliferative or autoimmune diseases has been reported or strongly suggested in all six published cases. Here, we report a young patient with severe idiopathic erythroblastic synartesis without an underlying disease, who was successfully treated with rituximab, an anti‐CD20 monoclonal antibody. The patient received rituximab at a dose of 375 mg/m2 once weekly for 4 wk after failure of both immunosuppressive therapies with corticosteroids and intravenous immunoglobulins. At a follow‐up of 30 months after treatment, the patient is still in continuous complete remission without any further treatment, suggesting that rituximab may induce prolonged remissions and eventually cure in this rare disease.
Atopic eczema or atopiform dermatitis
Authors: Bos Jan D. , Brenninkmeijer Elian E.A. , Schram Mandy E. , Middelkamp‐Hup Maritza A. , Spuls Phyllis I. , Smitt J. Henk Sillevis 
Published: January 2010

Please cite this paper as: Atopic eczema or atopiform dermatitis. Experimental Dermatology 2010.Abstract:  Age period prevalence of atopic eczema (AE), a very common skin disease, has increased during the past decennia. This expansion seems to be ending in wealthy countries, while an increase is observed in developing nations, for which there is no firm explanation. Recent steps in understanding AE are the detection of skin barrier related filaggrin null mutations in approximately 25% of patients and the recognition of IL‐31 as a molecule possibly involved in the itch (pruritus). Also interesting are the recognition of thymus and activation‐regulated chemokine (TARC) and proliferating‐inducing ligand (APRIL), as being associated with AE severity and activity. Immunocentric and corneocentric views on pathogenesis (the inside‐outside paradigm) and the diagnostic entity atopiform dermatitis (AFD) are discussed here. We emphasize that diagnosing AE is not simple but challenging. We accentuate that a diagnosis of AE is only possible when there is allergen‐specific IgE. Advice as to the need for elimination of allergens and adjustment of lifestyle are only proficient in patients having atopy and true AE, not in those having AFD.
Heparanase activation induces epidermal hyperplasia, angiogenesis, lymphangiogenesis and wrinkles
Authors: Iriyama Shunsuke , Matsunaga Yukiko , Amano Satoshi 
Published: January 2010

Please cite this paper as: Heparanase activation induces epidermal hyperplasia, angiogenesis, lymphangiogenesis and wrinkles. Experimental Dermatology 2010; 19: 965–972.Abstract:  To clarify the difference between cutaneous responses to single and repeated barrier disruption, changes of epidermal gene expression were examined by using RT‐PCR. In repeatedly barrier‐disrupted skin, heparanase was specifically up‐regulated in epidermis. In addition, there was a marked decrease in heparan sulfate (HS) chains of perlecan in basement membrane at the dermal–epidermal junction (DEJ) compared with singly disrupted skin. HS chains form a reservoir for heparan sulfate‐binding growth factors. In repeatedly barrier‐disrupted skin, expression of vascular endothelial growth factor‐A (VEGF‐A), an angiogenic factor, was induced in epidermis, whereas thrombospondin‐1 (TSP‐1), an angiogenesis inhibitor, was down‐regulated, and concomitantly blood vessels were elongated and enlarged in dermis. Expression of VEGF‐C, a lymphangiogenesis factor, was augmented in epidermis of repeatedly barrier‐disrupted skin, concomitantly with an increase in the number and size of lymphatic vessels. Topical application of a synthetic heparanase inhibitor, 1‐[4‐(1H‐benzoimidazol‐2‐yl)phenyl]‐3‐[4‐(1H‐benzoimidazol‐2‐yl)phenyl]urea, to skin after barrier disruption significantly suppressed wrinkle formation, degradation of HS chains in the basement membrane, epidermal hyperplasia and the changes of blood and lymphatic vessels. These results suggest that chronic barrier disruption activates heparanase and induces gene expression changes, leading to increased growth factor interaction between epidermis and dermis, and facilitating various cutaneous changes, including wrinkle formation.
Alopecia in IL‐10‐deficient mouse pups is c‐kit‐dependent and can be triggered by iron deficiency
Authors: Vanderford Deborah A. , Greer Paula K. , Sharp Julie M. , Chichlowski Maciej , Clayburn Rouse D. , Angelica Selim M. , Hale Laura P. 
Published: January 2010

Please cite this paper as: Alopecia in IL‐10‐deficient mouse pups is c‐kit‐dependent and can be triggered by iron deficiency. Experimental Dermatology 2010; 19: 518–526.Abstract:  Hair loss (alopecia) can result from a variety of metabolic, endocrine, immunologic, and environmental causes. This investigation was undertaken to determine the mechanisms underlying the sporadic development of alopecia in litters from C57BL/6 interleukin‐10‐deficient (Il10−/−) mice. All pups in affected litters demonstrated alopecia by postnatal days 17–19, with hair loss from their trunks but not from their head, base of tail, or feet. Histopathology revealed distorted hair follicles containing broken hair shafts and prominent dermal infiltrates containing increased numbers of activated mast cells. Hair re‐growth began soon after weaning, suggesting that the alopecia was triggered by factors transmitted during lactation. Milk from Il10−/− dams induced macrophage secretion of pro‐inflammatory cytokines in vitro regardless of whether or not their pups developed alopecia. Feeding dams a diet containing 3–6 ppm iron increased the percentage of litters with alopecia to 100% for pups with mast cells, with 0% alopecia in mast cell‐deficient pups. When dams were fed a diet containing 131 ppm iron, significantly lower haemoglobin and hematocrit values were observed in pups from litters with alopecia (71%; 5 of 7 litters) compared to litters without alopecia. Genetic or pharmacologic inhibition of c‐kit that resulted in depletion of mast cells in pups prevented hair loss in at‐risk litters. These studies demonstrate that maternal iron‐restricted diets enhance the incidence of alopecia in IL‐10‐deficient mouse pups and suggest mast cells as potential effector cells. Further studies are indicated to further explore the mechanisms involved and to determine how mast cells may contribute to alopecia in humans.
Detection and identification of rumen bacteria constituting a fibrolytic consortium dominated by Fibrobacter succinogenes
Authors: SHINKAI Takumi , UEKI Takaaki , KOBAYASHI Yasuo 
Published: January 2010

ABSTRACTA fibrolytic consortium, dominated by the rumen cellulolytic bacterium Fibrobacter succinogenes, was artificially constructed on hay stems to detect and identify rumen bacteria that can potentially interact with F. succinogenes. Consortium‐bacterial members were determined by DGGE and sequencing analysis targeted bacterial 16S rDNA. An artificial consortium was formed in a 2‐step incubation of hay stems; the first step with group 1, 2 or 3 F. succinogenes strains, the second step with rumen fluid. After consortium formation, morphologically different bacteria were observed in association with F. succinogenes. DGGE exhibited more than 30 bands, the pattern of which depended on the F. succinogenes group. Sequencing suggested that Butyrivibrio fibrisolvens, Pseudobutyrivibrio ruminis, Clostridium sp., F. succinogenes group 2, Prevotella ruminicola and unclassified Bacteroides were prominent in the group 1 consortium and that Treponema bryantii, B. fibrisolvens, Acinetobacter sp, and Wolinella succinogenes were prominent in the group 2 consortium. However, in the group 3 consortium, F. succinogenes‐like bacteria were microscopically undetectable, whereas cellulolytic Ruminococcus albus and F. succinogenes group 1 were prominent, suggesting that the group 3 cannot be a core member of this consortium. This study is the first attempt to identify bacterial members of a fibrolytic consortium dominated by a specific bacterium.
Collagen content and architecture of the pectoralis muscle in male chicks and broilers reared under various nutritional conditions
Authors: DAS Chamali , ROY Bimol Chandra , OSHIMA Ichiro , MIYACHI Hideyuki , NISHIMURA Shotaro , IWAMOTO Hisao , TABATA Shoji 
Published: January 2010

ABSTRACTVarying chicken growth rates were induced with different nutritional regimes, and the collagen content and architecture of M. pectoralis (PT) were compared among 21‐day‐old chicks and broilers at 80 or 95 days of age. The percentage of muscle weight to live weight was higher in rapid growing chicks (8.4%) than slow growing chicks (6.3%). The 80‐day‐old broilers engaged in compensatory growth after the early slow growth period producing PT muscle at 11% of live weight. The 80‐ and 95‐day‐old chicks with restricted late growth after an early rapid growth period showed PT weight at 8% and 9% of live weight, respectively. Collagen content of the PT muscle markedly decreased from the chicks to the broilers. The collagen concentration was higher in the late‐growth restricted broilers (1.67–1.88 mg/g) than the compensatory growth broilers (1.01–1.10 mg/g). Collagen concentration did not differ between the rapid and slow growing chicks (2.72 and 2.94 mg/g). Scanning electron micrographs showed thick and thin perimysia, and honeycomb endomysia. In the perimysia, a stack layer of collagen platelets and a reticular layer of collagen fiber cords were distinguished and collagen baskets of adipocytes were observed. The perimysial collagen fibers became thicker during growth of the chicks to broilers. However, in the late‐growth restricted broilers, the perimysial collagen fibers seemed to have retarded development compared with the compensatory growth birds. The PT muscle of chickens develops optimally when body growth is enhanced. The PT muscle of the compensatory growth broilers had improved collagen architecture regardless of the marked decrease in collagen content.
Effects of glucose and amino acids on ghrelin secretion in sheep
Authors: SUGINO Toshihisa , KAWAKITA Yuko , FUKUMORI Rika , HASEGAWA Yoshihisa , KOJIMA Masayasu , KANGAWA Kenji , OBITSU Taketo , TANIGUCHI Kohzo 
Published: January 2010

ABSTRACTTwo experiments were conducted to elucidate the effects of post‐ruminal administration of starch and casein (Exp. 1), plasma amino acids concentrations (Exp. 2), and plasma glucose and insulin concentrations (Exp. 2) on plasma ghrelin concentrations in sheep. In Exp. 1, plasma ghrelin concentrations were determined by four infusion treatments (water, cornstarch, casein and cornstarch plus casein) in four wethers. Abomasal infusion of casein increased plasma α‐amino N (AAN) concentrations. Infusion of starch or casein alone did not affect plasma ghrelin concentrations, but starch plus casein infusion increased plasma levels of ghrelin, glucose and AAN. In Exp 2, we investigated the effects of saline or amino acids on ghrelin secretion in four wethers. Two hours after the initiation of saline or amino acid infusion into the jugular vein, glucose was also continuously infused to investigate the effects of blood glucose and insulin by hyper‐glycemic clump on plasma ghrelin concentrations. Infusion of amino acids alone raised plasma levels of ghrelin, but the higher plasma glucose and insulin concentrations had no effect on plasma ghrelin concentrations. These results suggest that high plasma levels of amino acids can stimulate ghrelin secretion, but glucose and insulin do not affect ghrelin secretion in sheep.
DEVELOPMENT OF A PREDICTIVE MODEL DESCRIBING THE GROWTH OF CRONOBACTER SAKAZAKII IN RECONSTITUTED POWDERED INFANT MILK FORMULA
Authors: JO SEO‐HEE , HEO SUN‐KYUNG , HA SANG‐DO 
Published: January 2010

ABSTRACTCronobacter sakazakii has been associated most frequently with illness in neonates, so a mathematical model was developed to predict the growth rate of C. sakazakii in infant milk based on temperature. Reconstituted powdered infant milk formulas (RIMFs) inoculated with C. sakazakii were incubated at 10, 20, 30 and 40C. The primary model showed a good fit (r2 = 0.9613–0.999) to a Gompertz equation to obtain growth rates and lag times (LTs) at each temperature. The specific growth rate (SGR) of C. sakazakii in the RIMF increased, and the LT decreased with increasing temperature. A secondary polynomial model was developed using SAS general linear analysis software. The secondary model was “ln SGR = −0.06581 + (0.00575 × temperature) + (0.00039 × temperature2).” The SGR predicted using this model increased with an increasing temperature. This secondary polynomial model was judged as appropriate based on the mean square error (MSE of the SGR model = 0.0002), the coefficient of determination (r2 of the SGR model = 0.9975), the bias factor (Bf of the SGR model = 1.0375) and the accuracy factor (Af of the SGR model = 1.0724). Reliable prediction of C. sakazakii growth rates in RIMF was based on temperature.PRACTICAL APPLICATIONSPredictive models allow quantitative estimation of microorganism growth. Predicted specific growth rates (SGRs) using our secondary model were similar to measured SGRs, and evaluation of mathematical/statistical adequacy of the predictive model showed reliable results (r2 = 0.9975, MSE = 0.0002, Bf = 1.0375, Af = 1.0724). This model may be of use to dairy producers for manufacture of safe products by controlling Cronobacter sakazakii growth without the need for detection of the organism.
EFFECT OF ACID ADAPTATION ON THERMAL TOLERANCE OF ESCHERICHIA COLI O157:H7 AND SALMONELLA ENTERICA IN MEAT SERUM
Authors: SINGH MANPREET , MULLINS HOLLY R. , SIMPSON SHAWNA M. , DICKSON JAMES S. 
Published: January 2010

ABSTRACTEscherichia coli O157:H7 and Salmonella were adapted to acidic environments by growing in tryptic soy broth with 1% glucose (TSB + 1%G). Thermal tolerance of acid‐adapted pathogens was evaluated in meat serum. Five‐strain cocktail of both bacteria were grown separately in TSB and TSB + 1%G for 24 h at 37C. Meat serum was prepared from irradiated ground beef, inoculated with either acid‐adapted or nonadapted E. coli O157:H7 and Salmonella, and heated at 58, 62 and 65C to determine D values. D values of acid‐adapted E. coli O157:H7 were higher (P < 0.05) than nonadapted cells at 58, 62 and 65C. Significantly higher (P < 0.05) D values of acid‐adapted Salmonella were observed at 58 and 62C, but not at 65C. D values were 22.46 and 10.59 min at 58C, 3.58 and 1.38 min at 62C, 1.02 and 0.75 min at 65C for acid‐adapted and nonadapted E. coli O157:H7, respectively. D values of acid‐adapted Salmonella were 9.36 min at 58C, 1.66 min at 62C and 1.14 min at 65C, whereas the nonadapted counterparts had D values of 6.44, 0.88 and 0.95 min at 58, 62 and 65C, respectively.PRACTICAL APPLICATIONSFoodborne pathogens exist in the environment in healthy and/or stressed forms and have the potential to contaminate food products. This research was conducted to determine the effectiveness of commonly used heat treatments to eliminate acid‐adapted Escherichia coli O157:H7 and Salmonella in ground beef. There is limited literature on the thermal tolerance of acid adaptation of foodborne pathogens in actual food systems and studies in the past have been conducted at mild heating temperatures, whereas effects of heating to temperatures that are more indicative of cooking temperatures have rarely been researched. This study stresses upon the need for regulatory agencies and large‐scale meat manufacturers to take into consideration the increased thermal tolerance of acid‐adapted pathogens, as healthy growing cultures in a laboratory medium may inaccurately represent their survival in natural food environment.
HIGH PREVALENCE OF EXTENDED‐SPECTRUM β‐LACTAMASES ESCHERICHIA COLI AND VANCOMYCIN‐RESISTANT ENTEROCOCCI ISOLATES FROM CHICKEN PRODUCTS. A PROBLEM OF PUBLIC HEALTH
Authors: COSTA LUÍS , RADHOUANI HAJER , GOMES CLÁUDIA , IGREJAS GILBERTO , POETA PATRÍCIA 
Published: January 2010

ABSTRACT Twenty‐nine chicken products were acquired from different supermarkets in Portugal during September to December 2007 and were analyzed for extended‐spectrum β‐lactamases (ESBL) Escherichia coli and vancomycin‐resistant enterococci (VRE). Cefotaxime‐resistant E. coli isolates were recovered in 27 of 29 chicken samples representing 93% of the analyzed samples. The highest percentages of resistance (more than 50% of the isolates) were detected for ampicillin, nalidixic acid and tetracycline. VRE isolates were detected in 17 of 29 samples of chicken origin (59%) and were identified as Enterococcus durans (n = 15) and E. faecium (n = 2) with the highest percentages of resistance being detected for erythromycin, tetracycline, ampicillin and ciprofloxacin. Seven E. durans and the two E. faecium isolates recovered from chicken wings, gizzard and skin show gelatinase activity. The high rate of colonization of chicken products by these bacteria supports other studies suggesting that the food chain could be a source of ESBL and VRE colonization in humans representing a public health problem. PRACTICAL APPLICATIONSThe data indicate that chicken products may be contaminated with a high prevalence of extended‐spectrum β‐lactamases Escherichia coli and vancomycin‐resistant enterococci (VRE). It is important to mention that the isolates present a diversity of phenotypes of antimicrobial resistance, and half of the VRE isolates show also gelatinase activity, indicating that these animals may be a reservoir of bacteria showing virulence and increased resistance to antimicrobial agents, raising special concerns about their transmission to humans through the food chain.