bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cultivation and Serological Characterization of a Human Theiler's-Like Cardiovirus Associated with Diarrheal Disease
Authors: Chiu C. Y. , Greninger A. L. , Chen E. C. , Haggerty T. D. , Parsonnet J. , Delwart E. , DeRisi J. L. , Ganem D. 
Published: February 2010

ABSTRACT

Cardioviruses (e.g., Theiler's murine encephalomyelitis virus [TMEV]) are members of the
Picornaviridae
family that cause myocarditis and encephalitis in rodents. Recently, several studies have identified human cardioviruses, including Saffold virus (SAFV) and a related virus named human TMEV-like cardiovirus (HTCV). At least eight cardiovirus genotypes are now recognized, with SAFV and most strains of HTCV belonging to genotypes 1 and 2, respectively; genotype 2 strains are the most common in the population. Although a genotype 3 cardiovirus has recently been cultured (SAFV-3), the genotype 1 and 2 cardioviruses have been difficult to propagate
in vitro
, hindering efforts to understand their seroprevalence and pathogenicity. Here we present the isolation and characterization of a genotype 2 human cardiovirus (HTCV-UC6). Notably, successful cultivation of HTCV-UC6 from stool required the addition of cytokine-blocking antibodies to interrupt downstream antiviral pathways. Unlike SAFV-3, HTCV-UC6 exhibited slow replication kinetics and demonstrated only a moderate cytopathic effect. Serologic assays revealed that 91% of U.S. adults carry antibodies to the genotype 2 cardioviruses, of which 80% generate neutralizing antibodies, in agreement with previous data showing that cardiovirus infection is widespread in humans. We also demonstrate an acute cardiovirus seroconversion event in a child with diarrhea and vomiting, thus reporting for the first time evidence linking cardiovirus infection to diarrheal disease in humans.
Exposure to polycyclic aromatic hydrocarbons (PAHs), mutagenic aldehydes and particulate matter during pan frying of beefsteak
Authors: Sjaastad Ann Kristin , Jørgensen Rikke Bramming , Svendsen Kristin 
Published: February 2010

ObjectivesCooking with gas or electric stoves produces fumes, especially during frying, that contain a range of harmful and potentially mutagenic compounds as well as high levels of fine and ultrafine particles. The aim of this study was to see if polycyclic aromatic hydrocarbons (PAHs) and higher mutagenic aldehydes which were collected in the breathing zone of the cook, could be detected in fumes from the frying of beefsteak.MethodsThe frying was performed in a model kitchen in conditions similar to those in a Western European restaurant kitchen. The levels of PAHs (16 EPA standard) and higher aldehydes (trans,trans-2,4-decadienal, 2,4-decadienal, trans-trans-2,4-nonadienal, trans-2-decenal, cis-2-decenal, trans-2-undecenal, 2-undecenal) were measured during frying on an electric or gas stove with margarine or soya bean oil as the frying fat. The number concentration of particles <100 nm in size (ultrafine) was also measured, as well as the mass concentration of total particulate matter.ResultsLevels of naphthalene were in the range of 0.15–0.27 μg/m3air. Measured levels of mutagenic aldehydes were between non-detectable and 61.80 μg/m3air. The exposure level of total aerosol was between 1.6 and 7.2 mg/m3air. Peak number concentrations of ultrafine particles were in the range of 6.0×104–89.6×104particles/cm3air.ConclusionNaphthalene and mutagenic aldehydes were detected in most of the samples. The levels were variable, and seemed to be dependent on many factors involved in the frying process. However, according to the present results, frying on a gas stove instead of an electric stove causes increased occupational exposure to some of the components in cooking fumes which may cause adverse health effects.
INFORMATION LOSS FOR SYNTHETIC DATA THROUGH FUZZY CLUSTERING
Authors: LADRA SUSANA , TORRA VICENÇ 
Published: February 2010

Synthetic data generators are one of the methods used in privacy preserving data mining for ensuring the privacy of the individuals when their data are published. Synthetic data generators construct artificial data from some models obtained from the original data. Such models are mainly based on statistics and, typically, do not take into account other aspects of interest in artificial intelligence. In this paper we study whether one family of such synthetic data generators (the IPSO family) preserves the properties of the data that are of interest when users plan to apply clustering techniques. In particular, we study the effect of such synthetic data generators on fuzzy clustering. That is, we study the information loss data suffer when the original data are replaced by the synthetic ones.
Strong and Weak Convergence Theorems for Common Solutions of Generalized Equilibrium Problems and Zeros of Maximal Monotone Operators
Authors: Zeng L-C , Ansari QH , Shyu DavidS , Yao J-C 
Published: February 2010

AbstractThe purpose of this paper is to introduce and study two modified hybrid proximal-point algorithms for finding a common element of the solution set EP of a generalized equilibrium problem and the set "Equation missing" for two maximal monotone operators "Equation missing" and "Equation missing" defined on a Banach space "Equation missing". Strong and weak convergence theorems for these two modified hybrid proximal-point algorithms are established.
hsa-miR-29c* Is Linked to the Prognosis of Malignant Pleural Mesothelioma
Authors: Pass Harvey I. , Goparaju Chandra , Ivanov Sergey , Donington Jessica , Carbone Michele , Hoshen Moshe , Cohen Dalia , Chajut Ayelet , Rosenwald Shai , Dan Harel , Benjamin Sima , Aharonov Ranit 
Published: February 2010

Abstract
The inability to forecast outcomes for malignant mesothelioma prevents clinicians from providing aggressive multimodality therapy to the most appropriate individuals who may benefit from such an approach. We investigated whether specific microRNAs (miR) could segregate a largely surgically treated group of mesotheliomas into good or bad prognosis categories. A training set of 44 and a test set of 98 mesothelioma tumors were analyzed by a custom miR platform, along with 9 mesothelioma cell lines and 3 normal mesothelial lines. Functional implications as well as downstream targets of potential prognostic miRs were investigated. In both the training and test sets, hsa-miR-29c* was an independent prognostic factor for time to progression as well as survival after surgical cytoreduction. The miR was expressed at higher levels in epithelial mesothelioma, and the level of this miR could segregate patients with this histology into groups with differing prognosis. Increased expression of hsa-miR-29c* predicted a more favorable prognosis, and overexpression of the miR in mesothelioma cell lines resulted in significantly decreased proliferation, migration, invasion, and colony formation. Moreover, major epigenetic regulation of mesothelioma is mediated by hsa-miR-29c* and was shown through downregulation of DNA methyltransferases as well as upregulation of demethylating genes. A single miR has the potential to be a prognostic biomarker in mesothelioma, and validation of these findings as well as investigation of its downstream targets may give insight for potential therapies in the future. Cancer Res; 70(5); 1916–24
Evaluation of Treatment-Associated Inflammatory Response on Diffusion-Weighted Magnetic Resonance Imaging and 2-[18F]-Fluoro-2-Deoxy-d-Glucose-Positron Emission Tomography Imaging Biomarkers
Authors: Galbán Craig J. , Bhojani Mahaveer S. , Lee Kuei C. , Meyer Charles R. , Van Dort Marcian E. , Kuszpit Kyle K. , Koeppe Robert A. , Ranga Rajesh , Moffat Bradford A. , Johnson Timothy D. , Chenevert Thomas L. , Rehemtulla Alnawaz , Ross Brian D. 
Published: February 2010

Abstract
Purpose: Functional imaging biomarkers of cancer treatment response offer the potential for early determination of outcome through the assessment of biochemical, physiologic, and microenvironmental readouts. Cell death may result in an immunologic response, thus complicating the interpretation of biomarker readouts. This study evaluated the temporal effect of treatment-associated inflammatory activity on diffusion magnetic resonance imaging and 2-[18F]-fluoro-2-deoxy-d-glucose-positron emission tomography imaging (FDG-PET) biomarkers to delineate the effects of the inflammatory response on imaging readouts.
Experimental Design: Rats with intracerebral 9L gliosarcomas were separated into four groups consisting of control, an immunosuppressive agent dexamethasone (Dex), 1,3-bis(2-chloroethyl)-1-nitrosourea (BCNU), and BCNU+Dex. Animals were imaged using diffusion-weighted magnetic resonance imaging and FDG-PET at 0, 3, and 7 days posttreatment.
Results: In the BCNU- and BCNU+Dex-treated animal groups, diffusion values increased progressively over the 7-day study period to ∼23% over baseline. The FDG percentage change of standard uptake value decreased at day 3 (−30.9%) but increased over baseline levels at day 7 (+20.1%). FDG-PET of BCNU+Dex-treated animals were found to have percentage of standard uptake value reductions of −31.4% and −24.7% at days 3 and 7, respectively, following treatment. Activated macrophages were observed on day 7 in the BCNU treatment group with much fewer found in the BCNU+Dex group.
Conclusions: Results revealed that treatment-associated inflammatory response following tumor therapy resulted in the accentuation of tumor diffusion response along with a corresponding increase in tumor FDG uptake due to the presence of glucose-consuming activated macrophages. The dynamics and magnitude of potential inflammatory response should be considered when interpreting imaging biomarker results. Clin Cancer Res; 16(5); 1542–52
The FLT3 Internal Tandem Duplication Mutation Is a Secondary Target of the Aurora B Kinase Inhibitor AZD1152-HQPA in Acute Myelogenous Leukemia Cells
Authors: Grundy Martin , Seedhouse Claire , Shang Shilli , Richardson Jaineeta , Russell Nigel , Pallis Monica 
Published: February 2010

AbstractAurora kinases play an essential role in orchestrating chromosome alignment, segregation, and cytokinesis during mitotic progression and both aurora-A and B are frequently overexpressed in a variety of human malignancies. In this study, we report the effects of AZD1152-HQPA, a highly selective inhibitor of aurora-B kinase, in acute myeloid leukemia (AML) cell lines and primary samples. We show that AZD1152-HQPA inhibits the phosphorylation of Histone H3 (pHH3) on serine 10 resulting in polyploid cells, apoptosis, and loss of viability in a panel of AML cell lines. We also show that AZD1152-HQPA sensitivity in our cell lines is irrespective of p53 status and the FLT3-ITD–expressing MOLM-13 and MV4-11 cell lines are particularly sensitive to AZD1152-HQPA. Internal tandem duplications (ITD) within the FLT3 tyrosine kinase receptor are found in ∼25% of AML patients and are associated with a poor prognosis. Here, we report that AZD1152-HQPA directly targets phosphorylated FLT3 along with inhibiting its downstream target phospho–signal transducer and activator of transcription 5 (STAT5) in the FLT3-ITD cell lines. We show pHH3 expression in primary AML blasts and its inhibition by AZD1152-HQPA at low doses in all of our primary samples tested. AZD1152-HQPA inhibits the clonogenic potential of primary AML samples, with FLT3-ITD samples being the most sensitive (P = 0.029). FLT3-ITD primary samples are also more sensitive to pHH3 inhibition (P = 0.022) and are particularly sensitive to pSTAT5 downregulation after treatment with AZD1152-HQPA compared with FLT3 wild-type samples (P = 0.007). We conclude that mutant FLT3 is a secondary target of AZD1152-HQPA and that FLT3-ITD primary samples are particularly sensitive to the drug. Mol Cancer Ther; 9(3); 661–72
Solar Cycles in 150 Years of Global Sea Surface Temperature Data
Authors: Zhou Jiansong , Tung Ka-Kit 
Published: February 2010

AbstractThe purpose of the present work is to demonstrate that a solar cycle response exists in surface temperature using the longest global dataset available, which is in the form of 1854–2007 sea surface temperature (SST), with an emphasis on methods and procedures, data quality, and statistical tests and the removal of deterministic signals, such as volcano aerosol forcing and greenhouse gas warming. Using the method of composite-mean difference (CMD) projection, signals of warming during solar maximum and cooling during solar minimum years are found in the global SST over the 14 cycles, dispelling previous claims that the solar cycle response before 1920 is opposite to that of the modern era. The magnitude of the solar cycle response averaged over the oceans between 60°S and 60°N is about 0.1°C of warming for each W m−2 variation of the solar constant (but is slightly lower, at ~0.085°C, when periods of suspected bad data are averaged in, which is consistent with previous work). The signal is robust provided that the years near the Second World War are excluded, during which transitions from British ships to U.S. ships introduced warm bias in the SST, as recently pointed out by D. Thompson and his colleagues. Monte Carlo tests show that the extracted signal has less than 0.02% chance of being a random occurrence. This establishes the existence of a solar cycle response at the earth’s surface at high statistical confidence. Contamination of the signal by volcano aerosols is estimated using the multiple CMD inversion method and found to be small over this long record, although ENSO contamination varies depending on the period chosen but is also small.The multidecadal trend of response to solar forcing is found to account for no more than a quarter of the observed warming in SST during the past 150 yr, under a reasonable but unproven assumption that the climate response to secular solar forcing and to solar cycle forcing has the same spatial pattern.
An Evaluation Metric for Intraseasonal Variability and its Application to CMIP3 Twentieth-Century Simulations
Authors: Xavier Prince K. , Duvel Jean-Philippe , Braconnot Pascale , Doblas-Reyes Francisco J. 
Published: February 2010

AbstractThe intraseasonal variability (ISV) is an intermittent phenomenon with variable perturbation patterns. To assess the robustness of the simulated ISV in climate models, it is thus interesting to consider the distribution of perturbation patterns rather than only one average pattern. To inspect this distribution, the authors first introduce a distance that measures the similarity between two patterns. The reproducibility (realism) of the simulated intraseasonal patterns is then defined as the distribution of distances between each pattern and the average simulated (observed) pattern. A good reproducibility is required to analyze the physical source of the simulated disturbances. The realism distribution is required to estimate the proportion of simulated events that have a perturbation pattern similar to observed patterns. The median value of this realism distribution is introduced as an ISV metric. The reproducibility and realism distributions are used to evaluate boreal summer ISV of precipitations over the Indian Ocean for 19 phase 3 of the Coupled Model Intercomparison Project (CMIP3) models. The 19 models are classified in increasing ISV metric order. In agreement with previous studies, the four best ISV metrics are obtained for models having a convective closure totally or partly based on the moisture convergence. Models with high metric values (poorly realistic) tend to give (i) poorly reproducible intraseasonal patterns, (ii) rainfall perturbations poorly organized at large scales, (iii) small day-to-day variability with overly red temporal spectra, and (iv) less accurate summer monsoon rainfall distribution. This confirms that the ISV is an important link in the seamless system that connects weather and climate.
Atmospheric Response to the Gulf Stream: Seasonal Variations*
Authors: Minobe Shoshiro , Miyashita Masato , Kuwano-Yoshida Akira , Tokinaga Hiroki , Xie Shang-Ping 
Published: February 2010

Abstract
The atmospheric response to the Gulf Stream front in sea surface temperature is investigated using high-resolution data from satellite observations and operational analysis and forecast. Two types of atmospheric response are observed with different seasonality and spatial distribution.
In winter, surface wind convergence is strong over the Gulf Stream proper between Cape Hatteras and the Great Banks, consistent with atmospheric pressure adjustments to sea surface temperature gradients. The surface convergence is accompanied by enhanced precipitation and the frequent occurrence of midlevel clouds. Local evaporation and precipitation are roughly in balance over the Florida Current and the western Gulf Stream proper. In summer, strong precipitation, enhanced high clouds, and increased lightning flash rate are observed over the Florida Current and the western Gulf Stream proper, without seasonal surface convergence enhancement. For the precipitation maximum over the Florida Current, local evaporation supplies about half of the water vapor, and additional moisture is transported from the south on the west flank of the North Atlantic subtropical high.
Atmospheric heating estimated by a Japanese reanalysis reveals distinct seasonal variations. In winter, a shallow-heating mode dominates the Gulf Stream proper, with strong sensible heating in the marine atmospheric boundary layer and latent heating in the lower troposphere. In summer, a deep-heating mode is pronounced over the Florida Current and the western Gulf Stream proper, characterized by latent heating in the middle and upper troposphere due to deep convection. Possible occurrences of these heating modes in other regions are discussed.
Antarctic Intermediate Water and Subantarctic Mode Water Formation in the Southeast Pacific: The Role of Turbulent Mixing
Authors: Sloyan Bernadette M. , Talley Lynne D. , Chereskin Teresa K. , Fine Rana , Holte James 
Published: February 2010

Abstract
During the 2005 austral winter (late August–early October) and 2006 austral summer (February–mid-March) two intensive hydrographic surveys of the southeast Pacific sector of the Southern Ocean were completed. In this study the turbulent kinetic energy dissipation rate ε, diapycnal diffusivity κ, and buoyancy flux Jb are estimated from the CTD/O2 and XCTD profiles for each survey. Enhanced κ of O(10−3 to 10−4 m2 s−1) is found near the Subantarctic Front (SAF) during both surveys. During the winter survey, enhanced κ was also observed north of the “subduction front,” the northern boundary of the winter deep mixed layer north of the SAF. In contrast, the summer survey found enhanced κ across the entire region north of the SAF below the shallow seasonal mixed layer. The enhanced κ below the mixed layer decays rapidly with depth. A number of ocean processes are considered that may provide the energy flux necessary to support the observed diffusivity. The observed buoyancy flux (4.0 × 10−8 m2 s−3) surrounding the SAF during the summer survey is comparable to the mean buoyancy flux (0.57 × 10−8 m2 s−3) associated with the change in the interior stratification between austral summer and autumn, determined from Argo profiles. The authors suggest that reduced ocean stratification during austral summer and autumn, by interior mixing, preconditions the water column for the rapid development of deep mixed layers and efficient Antarctic Intermediate Water and Subantarctic Mode Water formation during austral winter and early spring.
Girls’ Education as Freedom?
Authors: Dutt Shushmita 
Published: February 2010

This article examines the efforts being made by the government and others concerned about the education of adolescent girls left out of the formal educational system in India. The Alternate Learning Systems (ALS) has proved more useful and relevant than the regular government elementary school because there are transformatory inputs that help to shape the girls’ thinking, analytical skills and confidence. Yet, even this does not ensure that girls convert their educational resources into tangible gains. Searching for solutions, the article then examines the Capability Approach (CA) that conceptualizes education as enhancing individual capability. Girls would require support from an inclusive economic environment, enabling social attitudes and effective governance structures. The article therefore urges that unless state and society proactively work to put these supports in place, girls will be unable to convert their knowledge and skills towards achieving a better quality of life.
Micro-computed tomography-based phenotypic approaches in embryology: procedural artifacts on assessments of embryonic craniofacial growth and development
Authors: Schmidt Eric J , Parsons Trish E , Jamniczky Heather A , Gitelman Julian , Trpkov Cvett , Boughner Julia C , Logan C Cairine , Sensen Christoph W , Hallgrímsson Benedikt 
Published: February 2010

Abstract

Background
Growing demand for three dimensional (3D) digital images of embryos for purposes of phenotypic assessment drives implementation of new histological and imaging techniques. Among these micro-computed tomography (μCT) has recently been utilized as an effective and practical method for generating images at resolutions permitting 3D quantitative analysis of gross morphological attributes of developing tissues and organs in embryonic mice. However, histological processing in preparation for μCT scanning induces changes in organ size and shape. Establishing normative expectations for experimentally induced changes in size and shape will be an important feature of 3D μCT-based phenotypic assessments, especially if quantifying differences in the values of those parameters between comparison sets of developing embryos is a primary aim. Toward that end, we assessed the nature and degree of morphological artifacts attending μCT scanning following use of common fixatives, using a two dimensional (2D) landmark geometric morphometric approach to track the accumulation of distortions affecting the embryonic head from the native, uterine state through to fixation and subsequent scanning.

Results
Bouin's fixation reduced average centroid sizes of embryonic mouse crania by approximately 30% and substantially altered the morphometric shape, as measured by the shift in Procrustes distance, from the unfixed state, after the data were normalized for naturally occurring shape variation. Subsequent μCT scanning produced negligible changes in size but did appear to reduce or even reverse fixation-induced random shape changes. Mixtures of paraformaldehyde + glutaraldehyde reduced average centroid sizes by 2-3%. Changes in craniofacial shape progressively increased post-fixation.

Conclusions
The degree to which artifacts are introduced in the generation of random craniofacial shape variation relates to the degree of specimen dehydration during the initial fixation. Fixation methods that better maintain original craniofacial dimensions at reduced levels of dehydration and tissue shrinkage lead to the progressive accumulation of random shape variation during handling and data acquisition. In general, to the degree that embryonic organ size and shape factor into μCT-based phenotypic assessments, procedurally induced artifacts associated with fixation and scanning will influence results. Experimental designs will need to address these significant effects, either by employing alternative methods that minimize artifacts in the region of focus or in the interpretation of statistical patterns.