bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mechanisms regulating the nuclear translocation of p38 MAP kinase
Authors: Gong Xiaowei , Ming Xiaoyan , Deng Peng , Jiang Yong 
Published: May 2010

Abstractp38 mitogen‐activated protein kinase (MAPK) is of fundamental importance in a cell's response to environmental stresses, cytokines and DNA damage. p38 resides in the cytoplasm of resting cells, and translocates into the nucleus upon activation, yet the exact mechanisms remain largely unclear. We show here that the phosphorylation‐dependent nuclear translocation of p38 is a common phenomenon when cells are stimulated with various stresses. On the other hand, the nuclear export of p38 requires its dephosphorylation, and it is exported both in a MK2‐dependent and a nuclear export signal (NES)‐independent manner. Although different p38‐regulated/activated protein kinase (PRAK) mutants all dictate the intracellular localization of p38, results from a PRAK‐deficient cell line indicate that it plays no role in this process. Microtubule depolymerizing reagent nocodazole and dynein inhibitor EHNA both block the nuclear translocation of p38, demonstrating roles for microtubules and dynein in p38 transport. Taken together, stress‐induced nuclear accumulation of p38 is a phosphorylation‐dependent, microtubule‐ and dynein‐associated process. J. Cell. Biochem. 110: 1420–1429, 2010. © 2010 Wiley‐Liss, Inc.
The expression of functional toll‐like receptor 4 is associated with proliferation and maintenance of stem cell phenotype in endothelial progenitor cells (EPCs)
Authors: He Jin , Xiao Zhilin , Chen Xiaobin , Chen Meifang , Fang Li , Yang Mei , Lv Qingshan , Li Yuehui , Li Guancheng , Hu Jinyue , Xie Xiumei 
Published: May 2010

AbstractEndothelial dysfunction is involved in various cardiovascular diseases such as atherosclerosis. Endothelial progenitor cells (EPCs) contribute to re‐endothelialization and neo‐vascularization, and the increase of EPCs in peripherial circulation benefits the prognosis of cardiovascular disease. However, little is known about the biological stimuli that initiate the proliferation and the maintenance of stem cell phenotype of EPCs. Here we reported that human umbilical vein blood derived EPCs expressed gene transcripts coding for Toll‐like receptor (TLR) 1–6, TLR8–10, TLR4 co‐receptor CD14, and myeloid differentiation factor 88 (MyD88), a TLR adaptor molecule. Protein expression of TLR2, 4, CD14, and MyD88 was also detected by FACS or Western blot. The activation of TLR4 by LPS modulated the expression of TLRs, induced the phosphorylation of NF‐κB, P38, and ERK42/44, and up‐regulated the gene expression of cytokines IL‐8, IFN‐α, IFN‐β, and TNF‐α, suggesting EPCs expressed functional TLR4. Unexpectedly LPS treatment failed to induce apoptosis in EPCs, but instead promoted cell proliferation of EPCs. Furthermore, the treatment of EPCs with LPS up‐regulated stem cell markers AC133 and CD34 in both mRNA and protein levels, and down‐regulated the protein expression of differential marker eNOS. These results suggested that TLR4 functions to maintain the stem cell phenotype of EPCs and enlarge its population, which reveals a novel aspect of the multiple‐faced TLR biology, and may open new prospects for using TLR4 agonists to promote the production of EPCs for clinical use. J. Cell. Biochem. 111: 179–186, 2010. © 2010 Wiley‐Liss, Inc.
HTLV‐1 basic leucine‐zipper factor, HBZ, interacts with MafB and suppresses transcription through a Maf recognition element
Authors: Ohshima Takayuki , Mukai Risa , Nakahara Norie , Matsumoto Jun , Isono Osamu , Kobayashi Yusuke , Takahashi Satoru , Shimotohno Kunitada 
Published: May 2010

AbstractHTLV‐1 infection causes adult T‐cell leukemia (ATL). The development of ATL is thought to be associated with disruption of transcriptional control of cellular genes. HTLV‐1 basic leucine‐zipper (bZIP) factor, HBZ, is encoded by the complementary strand of the provirus. We previously reported that HBZ interacts with c‐Jun and suppresses its transcriptional activity. To identify the cellular factor(s) that interact with HBZ, we conducted a yeast two‐hybrid screen using full‐length HBZ as bait and identified MafB. HBZ heterodimerizes with MafB via each bZIP domain. Luciferase analysis revealed a significant decrease in transcription through Maf recognition element (MARE) in a manner dependent on the bZIP domain of HBZ. Indeed, production of full‐length HBZ in cells decreased the MARE‐bound MafB protein, indicating that HBZ abrogates the DNA‐binding activity of MafB. In addition, HBZ reduced the steady‐state levels of MafB, and the levels were restored by treatment with a proteasome inhibitor. These results suggest a suppressive effect of HBZ on Maf function, which may have a significant role in HTLV‐1 related pathogenesis. J. Cell. Biochem. 111: 187–194, 2010. © 2010 Wiley‐Liss, Inc.
Identification of RANBP16 and RANBP17 as novel interaction partners for the bHLH transcription factor E12
Authors: Lee Jun‐Ho , Zhou Shengli , Smas Cynthia M. 
Published: May 2010

AbstractThe ubiquitously expressed basic helix‐loop‐helix (bHLH) transcription factors E12 and E47, products of alternative splicing of the E2A/TCF3 gene, regulate diverse biological processes including cell growth, differentiation and development. To search for novel protein interactions for E12, we utilized the bHLH domain of E12 as a bait in yeast two‐hybrid screening. Yeast two‐hybrid, mammalian two‐hybrid, and co‐immunoprecipitation analyses demonstrate specific interaction of E12 with RANBP17, a novel member of the importin‐β superfamily; this interaction maps to the CRM1 homology region of RANBP17. Ectopic expression of RANBP17 leads to a ∼3‐fold increase in E2A/MyoD mediated transactivation of an E‐box regulated luciferase reporter gene. Interaction and transactivation studies also revealed similar functions for RANBP16/XPO7. Furthermore, ectopic expression of either RANBP16 or RANBP17 resulted in increased level of endogenous transcript for the cyclin‐dependent kinase inhibitor, p21Waf1/Cip1, a well‐characterized E2A target gene. Together, these biochemical and functional data reveal RANBP16 and RANBP17 as novel regulators of E2A protein action. J. Cell. Biochem. 111: 195–206, 2010. © 2010 Wiley‐Liss, Inc.
Mechanisms of adiponectin‐mediated COX‐2 induction and protection against iron injury in mouse hepatocytes
Authors: Lee Fei‐Peng , Jen Chih‐Yu , Chang Chih‐Cheng , Chou Ying , Lin Heng , Chou Chih‐Ming , Juan Shu‐Hui 
Published: May 2010

AbstractAdiponectin (APN)‐mediated cyclooxygenase (COX)‐2 induction is known to have various protective effects on cardiovascular diseases. However, the molecular mechanisms of APN‐mediated COX‐2 induction and its protection against iron‐mediated injury in hepatocytes are still unclear. Herein, we show that AMP‐mediated peroxisome proliferator‐activated receptor (PPAR)α activation was attributable to COX‐2 induction by APN, which was further confirmed by identifying novel functional PPAR responsive elements (PPREs) in the mouse COX‐2 promoter region. Prostaglandin (PG)I2 and PGE2, metabolites of COX‐2, time‐dependently increased in hepatocytes treated with APN. Interestingly, beraprost and misoprostol, respective agonists for PGI2 and PGE2, mimicked the protective effects of APN in iron‐mediated inflammation in hepatocytes. The iron dextran‐activated nuclear factor (NF)‐κB pathway was correlated with the increased production of inflammatory cytokines including tumor necrosis factor‐α, intercellular adhesion molecule‐1, and monocyte chemotactic protein‐1. This was eliminated by administration of APN, whereas blockage of PPARα activation, an upstream regulator of COX‐2 induction by APN, and COX‐2 activation reversed the anti‐inflammatory effect of APN, suggesting the crucial role of COX‐2 in this event. Herein, we demonstrate that APN‐mediated COX‐2 induction through a PPARα‐dependent mechanism, and COX‐2 exerted an anti‐inflammatory effect of APN in hepatocytes subjected to iron challenge. J. Cell. Physiol. 224: 837–847, 2010. © 2010 Wiley‐Liss, Inc.
Reactive oxygen species control senescence‐associated matrix metalloproteinase‐1 through c‐Jun‐N‐terminal kinase
Authors: Dasgupta Jaya , Kar Supriya , Liu Rong , Joseph Joy , Kalyanaraman Balaraman , Remington S. James , Chen Ceshi , Melendez J. Andres 
Published: May 2010

AbstractThe lifetime exposure of organisms to oxidative stress influences many aging processes which involve the turnover of the extracellular matrix. In this study, we identify the redox‐responsive molecular signals that drive senescence‐associated (SA) matrix metalloproteinase‐1 (MMP‐1) expression. Precise biochemical monitoring revealed that senescent fibroblasts increase steady‐state (H2O2) 3.5‐fold (13.7–48.6 pM) relative to young cells. Restricting H2O2 production through low O2 exposure or by antioxidant treatments prevented SA increases in MMP‐1 expression. The H2O2‐dependent control of SA MMP‐1 is attributed to sustained JNK activation and c‐jun recruitment to the MMP‐1 promoter. SA JNK activation corresponds to increases and decreases in the levels of its activating kinase (MKK‐4) and inhibitory phosphatase (MKP‐1), respectively. Enforced MKP‐1 expression negates SA increases in JNK phosphorylation and MMP‐1 production. Overall, these studies define redox‐sensitive signaling networks regulating SA MMP‐1 expression and link the free radical theory of aging to initiation of aberrant matrix turnover. J. Cell. Physiol. 225: 52–62, 2010. © 2010 Wiley‐Liss, Inc.
A study on protonation equilibria in a series of amino‐1‐(substituted phenyl)pyrazoles
Authors: Garrone A. , Tironi C. , Fruttero R. , Gasco A. 
Published: May 2010

AbstractThe ionization constants (proton gain) of 3‐,4‐, and 5‐amino‐1‐(substituted phenyl)pyrazoles, have been measured in 10% v/v ethanol‐water. pKa values show a good linear dependence on Hammett substituent constants. The reaction constants Q are in keeping with the respective protonation sites in the three series of monocations. The uv spectra of 1‐(m‐nitrophenyl)pyrazole and 1‐(p‐methoxyphenyl)pyrazole and of their 3‐amino, 4‐amino, 5‐amino analogues recorded in acidic and neutral media, show that in the three series the first protonation site does not change with change in substituent at phenyl ring.
A stereochemical investigation of 2‐methyl‐ and 2,5‐dimethyl‐3‐phenyl‐1,3‐oxazolidines using NMR
Authors: Nishiyama Tomihiro , Nishikawa Takahiro , Yamada Fukiko 
Published: May 2010

AbstractThe stereochemical properties of two 2‐methyl‐3‐phenyl‐1,3‐oxazolidines and two of its 5‐methyl substituted and four of its 2,5‐dimethyl substituted derivatives have been investigated by pmr and cmr methods. The compounds of 2,5‐dimethyl‐3‐phenyl‐1,3‐oxazolidines exist in isomeric cis and trans forms at the two methyl groups on the heterocyclic ring.
Acetylenchemie 12. Mitt.: Weitere studien über die phasentransfer‐katalysierte umsetzung des 9(10H)‐acridinons mil alkinylhalogeniden
Authors: Reisch Johannes , Salehi‐Artimani Reza A. 
Published: May 2010

AbstractBei der phasentransfer‐katalysierten Umsetzung von 9(10H)‐Acridinon mil 1‐Brom‐2‐propin entsteht 10‐(2‐Propinyl)‐9(10H)‐acridinon. Aus diesem bilden sich via Prototropie 10‐(1,2‐Propadienyl)‐9(10H) acridinon und 10‐(1‐Propinyl)‐9(10H)‐acridinon. Unter den genannten Bedingungen führt 1‐Brom‐2‐butin zu 10‐(2‐Butinyl)‐9(10H)‐acridinon, 2‐Chlor‐3‐butin zu 10‐(l‐Methyl‐1,2‐propadienyl)‐9(10H)‐acridinon, 10‐(1‐Methyl‐2‐propinyl)‐9(10H)‐acridinon, 9‐(1‐Methyl‐2‐propinyloxy)‐acridin und 10‐[1‐Methyl‐3‐(3,4‐di‐methylphenyl‐2‐propinyl)]‐9(10H)‐acridinon. Die Entstehungsweise der Produkte wird experimentell abgesichert und mit früheren Befunden verglichen.
The reactions of C‐methylheterocycles with thionyl chloride. Part 3. The transformation of some five‐and six‐membered heterocycles
Authors: Al‐Shaar Adnan H. M. , Gilmour David W. , Lythgoe David J. , Mcclenaghan Ian , Ramsden Christopher A. 
Published: May 2010

AbstractReactions of various five‐and six‐membered C‐methylheterocycles with hot thionyl chloride gave chlorothio intermediates which were not characterized but which gave 1,2,4‐thiadiazole derivatives when reacted with amidines and gave thioamides when reacted with amines. When 2,3‐dimethyl‐6‐ethoxycarbonylimidazo‐[4,5‐b]pyridine (26) was treated with thionyl chloride a trisulphane 27 was isolated.
10th international symposium on the synthesis and applications of isotopes and isotopically labelled compounds—applications of isotopes in agriculture, nutrition and environmental research Session 11, Wednesday, June 17, 2009
Authors: McKillican Bruce , White Franklin 
Published: May 2010

AbstractThe preparation of carbon‐14 labeled compounds and their use in discovery and development of agrochemicals was discussed. Copyright © 2010 John Wiley & Sons, Ltd.
Characterization of conformational changes and noncovalent complexes of myoglobin by electrospray ionization mass spectrometry, circular dichroism and fluorescence spectroscopy
Authors: Lin Xin , Zhao Wenjie , Wang Xian 
Published: May 2010

AbstractElectrospray ionization mass spectrometry (ESI‐MS) was employed to monitor the heme release and the conformational changes of myoglobin (Mb) under different solvent conditions, and to observe ligand bindings of Mb. ESI‐MS, complemented by circular dichroism and fluorescence spectroscopy, was used to study the mechanism of acid‐ and organic solvent‐induced denaturation by probing the changes in the secondary and the tertiary structure of Mb. The results obtained show that complete disruption of the heme–protein interactions occurs when Mb is subjected to one of the following solution conditions: pH 3.2–3.6, or solution containing 20–30% acetonitrile or 40–50% methanol. Outside these ranges, Mb is present entirely in its native state (binding with a heme group) or as apomyoglobin (i.e. without the heme). Spectroscopic data demonstrate that the denaturation mechanism of Mb induced by acid may be significantly different from that by the organic solvent. Low pH reduces helices in Mb, whereas certain organic content level in solution results in the loss of the tertiary structure. ESI‐MS conditions were established to observe the H2O‐ and CO‐bound Mb complexes, respectively. H2O binding to metmyoglobin (17 585 Da), where the heme iron is in the ferric oxidation state, is observed in ESI‐MS. CO binding to Mb (17 595 Da), on the other hand, can be only observed after the heme iron is reduced to the ferrous form. Therefore, ESI‐MS combined with spectroscopic techniques provides a useful means for probing the formation of ligand‐binding complexes and characterizing protein conformational changes. Copyright © 2010 John Wiley & Sons, Ltd.
Interspecies comparison of mucosal wave properties using high‐speed digital imaging
Authors: Regner Michael F. , Robitaille Mark J. , Jiang Jack J. 
Published: May 2010

AbstractObjectives/Hypothesis:The purpose of this study was to compare the vocal fold vibratory characteristics of ex vivo bovine, canine, ovine, and porcine larynges to human male and female vocal fold vibrations to determine the best model organism for laryngeal studies concerning vibratory and kinetic characteristics.Study Design:Prospective experimental.Methods:High‐speed videos of phonation were gathered at 4,000 frames per second (fps) in the animal models and human high‐speed endoscopy data were gathered at 2,000 fps. Videos were converted into kymograms, and the amplitude, oscillation frequency, and phase difference of vocal fold vibration were measured.Results:No statistically significant differences were found with respect to frequency, amplitude, or phase difference between canines and humans. Porcines were not significantly different from human females but did have an oscillation frequency significantly different from human males. Ovine vibrational amplitudes were significantly different from humans, and bovine frequency and amplitude differed significantly from humans.Conclusions:Canine and porcine larynges are the most appropriate model specimens for laryngeal studies contingent on vibratory or kinetic properties of phonation.
Comparison of salvage and planned pharyngolaryngectomy with jejunal transfer for hypopharyngeal carcinoma after chemoradiotherapy
Authors: Kadota Hideki , Fukushima Junichi , Nakashima Torahiko , Kumamoto Yoshihiko , Yoshida Sei , Yasumatsu Ryuji , Shiratsuchi Hideki , Morita Masaru , Komume Shizuo 
Published: May 2010

AbstractObjectives/Hypothesis:Salvage surgery after definitive chemoradiotherapy is often associated with a higher rate of perioperative complications and poor prognosis. The objective of this study is to examine the safety and efficacy of free jejunal transfer after salvage pharyngolaryngectomy for patients with locally recurrent hypopharyngeal carcinoma after definitive chemoradiotherapy.Study Design:A retrospective analysis of patients with advanced hypopharyngeal carcinoma who underwent pharyngolaryngectomy and reconstruction using free jejunum.Methods:Forty patients who underwent pharyngolaryngectomy with jejunal transfer were included in this study. Fourteen patients underwent surgery after definitive chemoradiotherapy (the salvage‐surgery group), whereas 26 patients underwent surgery after planned preoperative chemoradiotherapy (the planned‐surgery group). The perioperative conditions, mortality, morbidity, functional outcomes, and oncologic outcomes in each group were compared.Results:The patients in the salvage‐surgery group lost an average of 9 kg in weight before surgery, which thus indicated a malnourished condition. However, the incidence of all perioperative complications did not differ significantly between the groups. All patients in both groups achieved oral intake without tube feeding, and the intervals to start oral intake were 12.8 days in the salvage‐surgery group and 15.6 days in the planned‐surgery group, which was not significantly different. The 5‐year disease‐free survival was 57.1% in the salvage‐surgery group and 50.4% in the planned‐surgery group, which was not significantly different.Conclusions:Salvage pharyngolaryngectomy and jejunal transfer can be performed safely and reliably for patients with locally recurrent hypopharyngeal carcinoma, and it is an excellent option after a failure of definitive chemoradiotherapy.
Cut‐off value for needle washout thyroglobulin in athyrotropic patients
Authors: Lee Young Hen , Seo Hyung Suk , Suh Sang‐il , Lee Nam Joon , Kim Jung Hyuk , Seol Hae Young , Lee Ju‐Han , Kwon Soon‐Young , Kim Nan Hee , Seo Ji A. , Yang Kyung‐Sook 
Published: May 2010

AbstractObjectives/Hypothesis:The purpose of this study was to determine the appropriate cut‐off value for fine needle aspiration‐thyroglobulin (FNA‐Tg) associated with postoperative recurrences and validate the diagnostic efficacy of FNA‐Tg in patients after total thyroidectomy compared with concomitant cytology (C).Study Design:Retrospective.Methods:We retrospectively evaluated the results of ultrasound‐guided FNAs performed for suspicious cervical lesions of 40 patients (male:female = 10:30; mean age, 44.0 years) after total thyroidectomy (mean duration, 89.1 months), to acquire the material for Tg and C analysis. After collection of the cytologic sample, we rinsed the same needle with 1 mL of normal saline for Tg radioimmunoassay.Results:Of 40 lesions (mean size, 0.89 cm; range, 0.3–3.5cm), 21 were confirmed as recurrences and 19 were nonrecurrences. The rates of nondiagnostic sampling and sensitivity and specificity of FNA‐C when diagnostic sampling was obtained were 40% (16/40), 100% (14/14), and 90.0% (9/10), respectively. According to receiver operating characteristic analysis, the optimal cut‐off value of FNA‐Tg was 4.1 ng/mL (sensitivity, 100% [21/21]; specificity, 100% [19/19], P < .05). Furthermore, in 16 inconclusive lesions due to nondiagnostic cytologic results, the FNA‐Tg results coincided with a final diagnosis (seven recurrences and nine nonrecurrences). The cases with maximum diameters <1 cm showed a significantly increased rate of nondiagnostic cytologic results, and the FNA‐Tg results were more helpful than lesions ≥1 cm in diagnosing a recurrence (P < .05).Conclusions:Measurement of FNA‐Tg is helpful for distinguishing recurrent from nonrecurrent lesions in the majority of patients who were previously treated for well‐differentiated papillary thyroid carcinoma.
Is the width of the labyrinthine portion of the fallopian tube implicated in the pathophysiology of Bell's palsy?: A prospective clinical study using computed tomography
Authors: Kefalidis Georgios , Riga Maria , Argyropoulou Paraskevi , Katotomichelakis Michael , Gouveris Charalampos , Prassopoulos Panos , Danielides Vasilios 
Published: May 2010

AbstractObjectives/Hypothesis:The pathogenetic mechanisms underlying Bell's palsy remain obscure, despite the extensive relevant research. Magnetic resonance imaging (MRI) studies have strongly indicated that facial nerve edema cannot be regarded as the sole etiologic factor, because it might persist long after full clinical recovery, or might be demonstrated in the clinically unaffected side or healthy controls. The aim of this study was to investigate the hypothesis that a narrow facial canal might be implicated in the pathophysiology of Bell's palsy.Study Design:Prospective clinical study.Methods:A high‐resolution computerized tomography of the temporal bone with 1‐mm thick contiguous axial sections was performed in 25 patients with unilateral Bell's palsy. The width of the fallopian tube was measured at the meatal foramen and the middle part of its labyrinthine segment.Results:When using paired Student t tests, the measured width of the affected ear was found significantly smaller than that of the unaffected side, both at the meatal foramen (P = .007) and at the middle part of the labyrinthine segment (P = .03).Conclusions:Bell's palsy seems to usually coincide with the narrower fallopian tube of the patient. This anatomical detail, supported by previous MRI studies, seems to indicate that an asymmetry between the right and left fallopian tube might be a necessary pathogenetic mechanism for the development of a facial nerve edema into Bell's palsy in the narrower fallopian canal. More studies on large healthy populations are needed before a notable facial canal asymmetry is linked to a higher risk for developing Bell's palsy.