bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effect of Lentinus sajor-caju on the chemical composition and antioxidant activity of highland barley straw under solid-state fermentation
Authors: Wang Yuqiong , Liao Yangci , Gou Changlong , Zhang Hang , Chen Liming , Bao Yuhong 
Published: May 2024

IntroductionThe efficient utilization of straw resources as animal feed has gained considerable attention. The objective of this study was to evaluate whether Lentinus sajor-caju treatment alters the chemical composition and antioxidant activity of highland barley straw and enhances its functional value as a ruminant feed.MethodsThe chemical composition, antioxidant capacity, and metabolomic profile of highland barley straw were determined after 21 days of solid-state fermentation with L. sajor-caju at 25°C. The differential metabolites between fermented and unfermented highland barley straw were identified by LC–MS and the relationship between the identified metabolites and antioxidant capacity was elucidated.ResultsThe results showed that, compared with untreated highland barley straw, the crude protein and ether extract contents were higher (51.55 and 76.43%, respectively) in highland barley straw after 21 days of incubation with L. sajor-caju, whereas the hemicellulose, cellulose, and acid detergent lignin contents were lower (2.48, 25.08, and 45%, respectively). The total antioxidant capacity was significantly higher in L. sajor-caju-treated than in untreated highland barley straw. In total, 600 differential metabolites (301 upregulated and 299 downregulated) were identified between L. sajor-caju-fermented and unfermented highland barley straw. Correlation analysis results showed that Fe2+ scavenging and total phenolic content were strongly correlated with total antioxidant capacity. Meanwhile, the differential flavonoid metabolites between fermented and unfermented highland barley straw were primarily associated with antioxidant activity, with kaempferol 3-xylosylglucoside, isoginkgetin, and rhoifolin being the most representative.ConclusionThus, this study demonstrates that L. sajor-caju could enhance the functional value of highland barley straw, showing the potential of L. sajor-caju for improving the utilization of agricultural straws in ruminants.
Enhancement on migration and biodegradation of Diaphorobacter sp. LW2 mediated by Pythium ultimum in soil with different particle sizes
Authors: Li Jialu , Hong Mei , Lv Jing , Tang Rui , Wang Ruofan , Yang Yadong , Liu Na 
Published: May 2024

IntroductionThe composition and structure of natural soil are very complex, leading to the difficult contact between hydrophobic organic compounds and degrading-bacteria in contaminated soil, making pollutants hard to be removed from the soil. Several researches have reported the bacterial migration in unsaturated soil mediated by fungal hyphae, but bacterial movement in soil of different particle sizes or in heterogeneous soil was unclear. The remediation of contaminated soil enhanced by hyphae still needs further research.MethodsIn this case, the migration and biodegradation of Diaphorobacter sp. LW2 in soil was investigated in presence of Pythium ultimum.ResultsHyphae could promote the growth and migration of LW2 in culture medium. It was also confirmed that LW2 was able to migrate in the growth direction and against the growth direction along hyphae. Mediated by hyphae, motile strain LW2 translocated over 3 cm in soil with different particle size (CS1, 1.0–2.0 mm; CS2, 0.5–1.0mm; MS, 0.25–0.5 mm and FS, &lt;0.25 mm), and it need shorter time in bigger particle soils. In inhomogeneous soil, hyphae participated in the distribution of introduced bacteria, and the total number of bacteria increased. Pythium ultimum enhanced the migration and survival of LW2 in soil, improving the bioremediation of polluted soil.DiscussionThe results of this study indicate that the mobilization of degrading bacteria mediated by Pythium ultimum in soil has great potential for application in bioremediation of contaminated soil.
The lesion core extent modulates the impact of early perfusion mismatch imaging on outcome variability after thrombectomy in stroke
Authors: Marburg Maria , Rudolf Linda F. , Matthis Christine , Neumann Alexander , Schareck Constantin , Schacht Hannes , Schulz Robert , Machner Björn , Schramm Peter , Royl Georg , Koch Philipp J. 
Published: May 2024

IntroductionDespite profitable group effects on functional outcomes after mechanical thrombectomy (MT) in large vessel occlusion (LVO), many patients with successful reperfusion show a non-favorable long-term outcome, highlighting the necessity to identify potential biomarkers predicting outcome variability. In this regard, the role of perfusion mismatch imaging for outcome variability in the early time window within 6 h after symptom onset is a matter of debate. We attempted to investigate under which conditions early perfusion mismatch imaging accounts for variability in functional outcomes after mechanical thrombectomy.Patients and methodsIn a retrospective single-center study, we examined 190 consecutive patients with LVO who were admitted to the Medical Center Lübeck within 6 h after symptom onset, all of whom underwent MT. Perfusion mismatch was quantified by applying the Alberta Stroke Program Early CT score (ASPECTS) on CT-measured cerebral blood flow (CBF-ASPECTS) and subtracting it from an ASPECTS application on cerebral blood volume (CBV-ASPECTS), i.e., ASPECTS mismatch. Using multivariate ordinal regression models, associations between ASPECTS mismatch and modified Rankin Scale (mRS) after 90 days were assessed. Furthermore, the interaction between ASPECTS mismatch and the core lesion volume was calculated to evaluate conditional associations.ResultsASPECTS mismatch did not correlate with functional outcomes when corrected for multiple influencing covariables. However, interactions between ASPECTS mismatch and CBV-ASPECTS [OR: 1.12 (1.06–1.18), p-value &lt; 0.001], as well as NCCT-ASPECTS [OR: 1.15 (1.06–1.25), p-value &lt; 0.001], did show a significant association with functional outcomes. Model comparisons revealed that, profoundly, in patients with large core lesion volumes (CBV-ASPECTS &lt; 6 or NCCT-ASPECTS &lt; 6), perfusion mismatch showed a negative correlation with the mRS.Discussion and conclusionPerfusion mismatch imaging within the first 6 h of symptom onset provides valuable insights into the outcome variability of LVO stroke patients receiving thrombectomy but only in patients with large ischemic core lesions.
The prognostic value of selective neck dissection in early-stage major salivary gland carcinoma: a population-based analysis
Authors: An Meiyu , Zuo Jiaxin , Yuan Fang , Xiong Ping 
Published: May 2024

ObjectiveThis population-based study aims to assess the survival benefits of selective neck dissection (SND) compared to neck observation in patients with T1/T2N0M0 major salivary gland malignancy (MSGC).MethodsWe conducted a retrospective review of T1/T2N0M0 MSGC patients who underwent primary tumor surgical extirpation with or without elective neck dissection in the Surveillance, Epidemiology, and End Results database (SEER) from 2004-2015. The impact of SND and clinical variables on overall survival (OS) and disease-specific survival (DSS) was evaluated using Univariate and Multivariate Cox proportional hazards regression models. Kaplan-Meier survival curves were generated, and survival rates were assessed via the log-rank test.ResultsOf 3778 post-operative T1-T2N0M0 MSGC patients, 2305 underwent elective neck dissection, while 1473 did not. Median follow-up was 106 months. Univariate and Multivariate analysis identified SND as a prognostic factor for OS in all the study population. After stratified analysis, we found that in the poorly high-grade (differentiated and undifferentiated) patients, the survival showed a significant OS and DSS benefit after receiving SND compared with the neck observations [HR for OS (95%CI): 0.571(0.446-0.731), P&lt;0.001] and [HR for DSS (95%CI): 0.564(0.385-0.826), P=0.003], other than in the well differentiated or moderately differentiated subgroup. Especially, when the pathological is squamous cell carcinoma, the results show that the people underwent SND had better prognosis, not only in OS [HR (95%CI): 0.532(0.322-0.876), P=0.013], but also in DSS [HR (95%CI): 0.330(0.136-0.797), P=0.014]. The multivariate analysis also yielded encouraging results, compared with neck observation, receiving SND bought about a significant independent OS (adjusted HR, 0.555; 95% CI, 0.328-0.941; P=0.029) and DSS (adjusted HR, 0.349; 95% CI, 0.142-0.858; P=0.022) advantage in high grade squamous cell carcinoma MSGC patients. The Kaplan-Meier survival curves also demonstrated that adjusted SND still had significantly better OS(P=0.029) and DSS(P=0.022) than the observation group in patients with high-grade squamous cell carcinoma of MSGC.ConclusionPoorly differentiated and undifferentiated T1/T2N0M0 major salivary gland malignancy treated with selective neck dissection demonstrated superior survival compared to neck observation, especially in the pathological subtype of squamous cell carcinoma. These findings suggest the potential benefits of multimodal therapy for appropriately selected patients, emphasizing significant clinical implications.
Systematic review of drug-drug interactions of delta-9-tetrahydrocannabinol, cannabidiol, and Cannabis
Authors: Nachnani Rahul , Knehans Amy , Neighbors Jeffrey D. , Kocis Paul T. , Lee Tzuo , Tegeler Kayla , Trite Thomas , Raup-Konsavage Wesley M. , Vrana Kent E. 
Published: May 2024

BackgroundThe recent exponential increase in legalized medical and recreational cannabis, development of medical cannabis programs, and production of unregulated over-the-counter products (e.g., cannabidiol (CBD) oil, and delta-8-tetrahydrocannabinol (delta-8-THC)), has the potential to create unintended health consequences. The major cannabinoids (delta-9-tetrahydrocannabinol and cannabidiol) are metabolized by the same cytochrome P450 (CYP) enzymes that metabolize most prescription medications and xenobiotics (CYP3A4, CYP2C9, CYP2C19). As a result, we predict that there will be instances of drug-drug interactions and the potential for adverse outcomes, especially for prescription medications with a narrow therapeutic index.MethodsWe conducted a systematic review of all years to 2023 to identify real world reports of documented cannabinoid interactions with prescription medications. We limited our search to a set list of medications with predicted narrow therapeutic indices that may produce unintended adverse drug reactions (ADRs). Our team screened 4,600 reports and selected 151 full-text articles to assess for inclusion and exclusion criteria.ResultsOur investigation revealed 31 reports for which cannabinoids altered pharmacokinetics and/or produced adverse events. These reports involved 16 different Narrow Therapeutic Index (NTI) medications, under six drug classes, 889 individual subjects and 603 cannabis/cannabinoid users. Interactions between cannabis/cannabinoids and warfarin, valproate, tacrolimus, and sirolimus were the most widely reported and may pose the greatest risk to patients. Common ADRs included bleeding risk, altered mental status, difficulty inducing anesthesia, and gastrointestinal distress. Additionally, we identified 18 instances (58%) in which clinicians uncovered an unexpected serum level of the prescribed drug. The quality of pharmacokinetic evidence for each report was assessed using an internally developed ten-point scale.ConclusionDrug-drug interactions with cannabinoids are likely amongst prescription medications that use common CYP450 systems. Our findings highlight the need for healthcare providers and patients/care-givers to openly communicate about cannabis/cannabinoid use to prevent unintended adverse events. To that end, we have developed a free online tool (www.CANN-DIR.psu.edu) to help identify potential cannabinoid drug-drug interactions with prescription medications.
Evaluation of a push-and-pull strategy using volatiles of host and non-host plants for the management of pear psyllids in organic farming
Authors: Czarnobai De Jorge Bruna , Koßmann Alicia , Hummel Hans E. , Gross Jürgen 
Published: May 2024

IntroductionPear decline (PD) is one of the most devastating diseases of Pyrus communis in Europe and North America. It is caused by the pathogen ‘Candidatus Phytoplasma pyri’ and transmitted by pear psyllids (Cacopsylla pyri, C. pyricola, and C. pyrisuga). Identifying attractant and repellent volatile organic compounds (VOCs) could improve the development of alternative plant protection measurements like push-pull or attract-and-kill strategies against pear psyllids. Our objective was to investigate which chemical cues of the host plant could influence the host-seeking behavior of pear psyllids, and if cedarwood (CWO) and cinnamon bark (CBO) essential oils could serve as repellents.Results and discussionBased on the literature, the five most abundant VOCs from pear plants elicited EAG responses in both C. pyri and C. pyrisuga psyllid species. In Y-olfactometer trials, single compounds were not attractive to C. pyri. However, the main compound mixture was attractive to C. pyri and C. pyrisuga females. CWO and CBO were repellent against C. pyri, and when formulated into nanofibers (NF), both were repellent in olfactometer trials. However, CBO nanoformulation was ineffective in masking the odors of pear plants. In a field trial, attractive, repellent CWO and blank formulated NF were inserted in attractive green sticky traps. C. pyri captures in traps with CWO NF were statistically lower than in traps with the attractive mixture. Nevertheless, no statistical differences in the numbers of caught specimens were observed between CWO NF and those captured in green traps baited with blank NF. Transparent traps captured fewer psyllids than green ones. In a second field study with a completed different design (push-and-count design), dispensers filled with CBO were distributed within the plantation, and attractive green sticky traps were placed around the plantation. The numbers of trapped pear psyllids increased significantly in the border of the treated plantation, showing that psyllids were repelled by the EOs in the plantation. Although further field evaluation is needed to assess and improve their effectiveness, our results show that these aromatic compounds, repellent or attractive both in nanoformulations and marking pen dispensers, offer great potential as an environmentally sustainable alternative to currently applied methods for managing pear decline vectors.
The effect of spiritual support on life satisfaction in Iranian patients with gastrointestinal cancer
Authors: Hamidi Sajad , Seyedfatemi Naeimeh , Abbasi Zahra , Ebrahimpour Fatemeh 
Published: May 2024

BackgroundThe diagnosis and treatment of gastrointestinal cancer not only impose significant physical challenges but also deeply affect patients emotionally and psychologically, significantly influencing their overall quality of life. Among the various factors that can positively impact life satisfaction in individuals facing gastrointestinal cancer, spirituality emerges as a crucial aspect. This study aimed to determine the effect of a spiritual intervention on life satisfaction in patients with gastrointestinal cancer.MethodsThis quasi-experimental study was conducted with two groups of 85 gastrointestinal cancer patients from two major hospitals in Tehran. The intervention group received spiritual support through social media for six sessions over three weeks to learn how to effectively improve their spiritual state. The control group received routine medical visits and care. The Satisfaction with Life Scale (SWLS) was used before and after the spiritual support in both the intervention and control groups. The research was approved by the institutional ethics committee.ResultsIn the pre-test stage, there was no significant difference in average life satisfaction between the intervention and control groups (t = 1.887, d = 0.30, p &gt; 0.05). However, positive changes occurred in the post-test stage. Notably, the disparity in average life satisfaction between the intervention and control groups was significant in the post-test stage (t = 13.118, d = 0.95, p &lt; 0.01). Furthermore, the result showed that the changes in life satisfaction in the intervention group were statistically significant (t = 11.854, d = 0.84, p &lt; 0.001). Changes in life satisfaction in the control group were not statistically significant in the pre-test stage compared to the post-test stage (t = 1.113, d = 0.10, p &gt; 0.05).ConclusionThe results can guide health care providers in dealing with the problems of cancer patients. Assessing patients’ spiritual needs and empowering them to promote their spiritual recovery and find meaning in their suffering can lead to improved quality of life and satisfaction with holistic care.
Socioeconomic factors, sleep timing and duration, and amygdala resting-state functional connectivity in children
Authors: Hansen Melissa , Simon Katrina R. , He Xiaofu , Steele Nick , Thomas Michael L. , Noble Kimberly G. , Merz Emily C. 
Published: May 2024

IntroductionReduced sleep health has been consistently linked with increased negative emotion in children. While sleep characteristics have been associated with neural function in adults and adolescents, much less is known about these associations in children while considering socioeconomic context. In this study, we examined the associations among socioeconomic factors, sleep duration and timing, and resting-state functional connectivity (rsFC) of the amygdala in children.MethodsParticipants were typically-developing 5- to 9-year-olds from socioeconomically diverse families (61% female; N = 94). Parents reported on children’s weekday and weekend bedtimes and wake-up times, which were used to compute sleep duration and midpoint. Analyses focused on amygdala-anterior cingulate cortex (ACC) connectivity followed by amygdala-whole brain connectivity.ResultsLower family income-to-needs ratio and parental education were significantly associated with later weekday and weekend sleep timing and shorter weekday sleep duration. Shorter weekday sleep duration was associated with decreased amygdala-ACC and amygdala-insula connectivity. Later weekend sleep midpoint was associated with decreased amygdala-paracingulate cortex and amygdala-postcentral gyrus connectivity. Socioeconomic factors were indirectly associated with connectivity in these circuits via sleep duration and timing.DiscussionThese results suggest that socioeconomic disadvantage may interfere with both sleep duration and timing, in turn possibly altering amygdala connectivity in emotion processing and regulation circuits in children. Effective strategies supporting family economic conditions may have benefits for sleep health and brain development in children.
Body mass index, smoking behavior, and depression mediated the effects of schizophrenia on chronic obstructive pulmonary disease: trans-ethnic Mendelian-randomization analysis
Authors: Ni Yao , Zhang DaWei , Tang Wenlong , Xiang Liming , Cheng Xiaoding , Zhang Youqian , Feng Yanyan 
Published: May 2024

BackgroundPrevious studies have highlighted the association between schizophrenia (SCZ) and chronic obstructive pulmonary disease (COPD), yet the causal relationship remains unestablished.MethodsUnder the genome-wide significance threshold (P&lt;5×10–8), data from individuals of European (EUR) and East Asian (EAS) ancestries with SCZ were selected for analysis. Univariable Mendelian randomization (MR) explored the causal relationship between SCZ and COPD. Linkage disequilibrium score (LDSC) regression was used to calculate genetic correlation, while multivariable and mediation MR further investigated the roles of six confounding factors and their mediating effects. The primary method utilized was inverse-variance weighted (IVW), complemented by a series of sensitivity analyses and false discovery rate (FDR) correction.ResultsLDSC analysis revealed a significant genetic correlation between SCZ and COPD within EUR ancestry (rg = 0.141, P = 6.16×10–7), with no such correlation found in EAS ancestry. IVW indicated a significant causal relationship between SCZ and COPD in EUR ancestry (OR = 1.042, 95% CI 1.013–1.071, P = 0.003, PFDR= 0.015). Additionally, replication datasets provide evidence of consistent causal associations(P &lt; 0.05 &amp; PFDR &lt; 0.05). Multivariable and mediation MR analyses identified body mass index (BMI)(Mediation effect: 50.57%, P = 0.02), age of smoking initiation (Mediation effect: 27.42%, P = 0.02), and major depressive disorder (MDD) (Mediation effect: 60.45%, P = 6.98×10–5) as partial mediators of this causal relationship. No causal associations were observed in EAS (OR = 0.971, 95% CI 0.875–1.073, P = 0.571, PFDR= 0.761) ancestry. No causal associations were found in the reverse analysis across the four ancestries (P &gt; 0.05 &amp; PFDR &gt; 0.05).ConclusionsThis study confirmed a causal relationship between SCZ and the risk of COPD in EUR ancestry, with BMI, smoking, and MDD serving as key mediators. Future research on a larger scale is necessary to validate the generalizability of these findings across other ancestries.
Childhood exposure to domestic violence: can global estimates on the scale of exposure be obtained using existing measures?
Authors: Harris Rebecca Jane , Channon Andrew Amos , Morgan Sara Afshar 
Published: May 2024

PurposeChildhood exposure to domestic violence and abuse (DVA) can lead to major short- and long-term effects on the victim. Despite this, there is no accepted measure for children’s experiences, with most existing measures being validated only in high income countries and not in low- and middle- income countries. As a result, international statistics are not comparable. This paper seeks to critically appraise existing measures and discuss whether any are fit-for-purpose on a global scale.MethodThe COSMIN PROMs approach was followed to critically appraise and compare the appropriateness of measures. A comprehensive literature search was undertaken in seven journal databases for measures mentioned in formally peer-reviewed articles exploring childhood exposure to DVA.ResultsA literature search resulted in the identification of 10 measures and, following criteria to only keep original measures and remove modifications, four measures which have been validated cross-culturally are discussed in detail in line with the COSMIN PROMs criterion: The Child Exposure to Domestic Violence Scale, Children’s Perception of Interparental Conflict Scale, Juvenile Victimization Questionnaire and The Violence Exposure Scale for Children. Strengths and limitations of each are discussed, along with any validations undertaken not in the country of origin.ConclusionDespite childhood exposure to DVA being an urgent research priority worldwide, the current measures to explore the extent of the issue are not validated cross-culturally, leading to concerns about comparisons across different population groups. The development and implementation of interventions to reduce the levels and effects of exposure relies heavily on cross-cultural comparisons, which may indicate different strategies are needed in different contexts. The lack of these validated comparisons is constraining advances, and the paper advocates for further efforts to be made in this regard.