bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Rapid Screening Method for Wound Dressing by Cell‐on‐a‐Chip Device
Authors: Zhao Qilong , Wang Shiwen , Xie Yunyan , Zheng Wenfu , Wang Zhuo , Xiao Le , Zhang Wei , Jiang Xingyu 
Published: June 2012

AbstractThis report demonstrates an in vitro method for screening wound dressing candidates that can minimize the use of animals for developing better methods for wound care. The development of materials and formulations for wound dressings, an important application of biomaterials, is laboriously and ethically challenging because of the use of a large number of animals. A method for rapid and effective screening of wound dressings in vitro, therefore, is in great need. A cell‐on‐a‐chip model was used to simulate the cutaneous wound in vitro and screen the performances of several electrospun fibrous wound dressings in enhancing wound healing. For comparison, the performances of wound dressings were also evaluated in a rat model. It was found that the results acquired by microchip model corroborates well with animal experiments. It is the first time, as far as we know, that a good correlation between in vitro and in vivo results is reported for fibrous wound dressings. The cell‐on‐a‐chip wound model we developed here may change the way that scientists screen candidates for wound dressings.
Polyvalent Mesoporous Silica Nanoparticle‐Aptamer Bioconjugates Target Breast Cancer Cells
Authors: Li Le‐Le , Yin Qian , Cheng Jianjun , Lu Yi 
Published: June 2012

AbstractSpatiotemporal control over the delivery of therapeutic agents is an outstanding challenge to cancer treatment. By taking advantage of recent advances in DNA aptamer biology and mesoporous silica nanotechnology, we report a general approach to design and fabricate controlled release drug delivery systems that are able to effectively target cancer cells. Specifically, polyvalent mesoporous silica nanocarriers‐aptamer bioconjugates were constructed; the high‐surface‐area nanoporous core allowed high drug loading and the surface‐conjugated aptamer facilitated the nanoparticle targeting of nucleolin overexpressed MCF‐7 cells. The efficient cancer‐cell‐specific fluorescent imaging and drug delivery of the bioconjugates outline the great potential for therapeutic applications.
Esterification Reaction of Polystyrene Modified with Maleic Anhydride in the Presence of Resin Catalyst
Authors: Okudan Ahmet , Baktir Serdar , Sagdic Ahmet 
Published: June 2012

AbstractIn this study, primary modification of polystyrene (PS) was carried out with maleic anhydride using the Friedel–Crafts reaction. Its secondary modification was realized through esterification of modified polystyrene (MPS) containing a carboxyl group with various alcohols in the presence of Amberlyst‐36 as a heterogeneous catalyst. The heterogeneous catalyst gave moderate yields, whereas the reaction catalyzed by a homogeneous catalyst did not occur. The amount of carboxyl groups in the structure of MPS and unreacted carboxyl groups in the structure of the esterified MPS (EMPS) was found by using a chemical titration method for the determination of esterification yield. Some important reaction parameters were assigned to optimize the esterification process. FT‐IR, 1H NMR, and thermogravimetric studies indicate that the esterification reaction was successfully realized for each alcohol in the presence of the heterogeneous catalyst. The results demonstrated that both the acylation reaction and esterification reaction can be carried out to obtain a significantly functionalized polymer. © 2012 Wiley Periodicals, Inc. Adv Polym Techn 32: E451–E461, 2013; View this article online at wileyonlinelibrary.com. DOI 10.1002/adv.21292
A genome‐wide single‐nucleotide polymorphism‐array can improve the prognostic stratification of the core binding factor acute myeloid leukemia
Authors: Huh Jungwon , Kim Hee‐Je , Jung Chul Won , Kim Hee‐Jin , Kim Sun‐Hee , Kim Yeo‐Kyeoung , Kim Hyeoung‐Joon , Shin Myung Geun , Moon Joon Ho , Sohn Sang Kyun , Kim Sung Hyun , Lee Won Sik , Won Jong Ho , Mun Yeung Chul , Kim Hawk , Park Jinny , Min Woo Sung , Kim Dong Hwan (Dennis) ,   ; on behalf of AML/MDS working party, Korean Society of Hematology
Published: June 2012

AbstractCore binding factor (CBF) AML with the D816 C‐KIT gene mutation demonstrate inferior treatment outcomes. However, the remaining cases without the D816 C‐KIT mutation imply a requirement of more sophisticated dissection of the patients according to their prognosis. In this study, we analyzed the prognostic value of a single nucleotide polymorphism array (SNP‐A) based karyotyping combined with metaphase cytogenetics (MC) to facilitate further stratification of CBF AML patients. A total of 98 CBF AML patients were included and genome‐wide Human SNP 6.0 Arrays (Affymetrix) were performed using marrow samples taken at diagnosis. Overall, 40 abnormal lesions were identified in 25 patients (26%). Survival of the patients with the abnormal lesion(s) detected by SNP‐A and/or MC was worse than those without lesions in terms of the 2‐year overall survival (OS; 57.5% vs. 76.4%, P = 0.028), event‐free (EFS; 45.7% vs. 66.2%, P = 0.072), and leukemia‐free survival (LFS; 49.0% vs. 77.4%, P = 0.015), specially in the subgroup with inv(16)/t(16;16) (40.9% vs. 80.2% OS, P = 0.040) and in the subgroup without the D816 C‐KIT mutation (61.6% vs. 82.7% OS, P = 0.038). Multivariate analysis confirmed the prognostic impact of the abnormal SNP‐A and/or MC lesion on EFS (HR 2.011, P = 0.047), and LFS (HR 3.231, P = 0.005) in the overall CBF AML. This study suggests that the combined use of SNP‐A with MC in the CBF AML can provide important prognostic value, especially in the inv(16)/t(16;16) subgroup or in the patients without the D816 C‐KIT mutation. Am. J. Hematol., 2012. © 2012 Wiley Periodicals, Inc.
Role of c‐Myb in the survival of pre B‐cell acute lymphoblastic leukemia and leukemogenesis
Authors: Sarvaiya Purvaba J. , Schwartz Jason R. , Hernandez Claudia P. , Rodriguez Paulo C. , Vedeckis Wayne V. 
Published: June 2012

AbstractAcute lymphoblastic leukemia (ALL) is the most common cancer in children. The current treatment protocol for ALL involves an intense chemotherapy regimen yielding cure rates of nearly 80%. However, new therapies need to be designed not only to increase the survival rate but also to combat the risk of severe therapy associated toxicities including secondary malignancies, growth problems, organ damage, and infertility. The c‐Myb proto‐oncogene is highly expressed in immature hematopoietic cells. In this study, we demonstrate that loss of c‐Myb itself decreased the viability of these leukemic cells. Additionally, the inhibition of c‐Myb caused a decrease in cell proliferation, significantly increased the number of cells in G0/G1 phase of the cell cycle, increased the sensitivity of pre‐B‐ALL cells to cytotoxic agents in vitro, and significantly delayed disease onset in a mouse model of leukemia. Furthermore, we demonstrate that Bcl‐2 is a target of c‐Myb in pre‐B‐ALL cells. Our results identify c‐Myb as a potential therapeutic target in pre‐B‐ALL and suggest that suppression of c‐Myb levels or activity, in combination with currently used therapies and/or dose reduction, may lead to a decrease in toxicity and an increase in patient survival rates. Because c‐Myb is aberrantly expressed in several other malignancies, targeting c‐Myb will have broad clinical applications. Am. J. Hematol., 2012. © 2012 Wiley Periodicals, Inc.
Proteintango: wie man den Partner einfängt
Authors: Rutkowska Anna , Schultz Carsten 
Published: June 2012

AbstractDie Bestimmung von Proteinfunktionen in lebenden Zellen ist essenziell, um Zellen und Organismen zu verstehen. Von besonderer Wichtigkeit ist dabei die Ermittlung und Beeinflussung von Protein‐Protein‐Interaktionen. In den letzten Jahren wurden mehrere Methoden entwickelt, um Protein‐Protein‐Interaktionen in lebenden Zellen zu messen bzw. zu induzieren. Daher sind jetzt Werkzeuge verfügbar, um intrazelluläre Abläufe spezifisch durch reversibles oder irreversibles Verknüpfen zu manipulieren. Dies kann neue Türen öffnen, um komplizierte Proteinnetzwerke im Detail zu analysieren. Hier beschreiben wir die verfügbaren Verknüpfungs‐ und Dimerisierungsreagentien und ihre Anwendungen.
Optimization of engineering and solvent resistive behavior of high vinyl acetate content EVA‐modified poly(ethylene‐co‐1‐octene) interpenetrating network blends using taguchi orthogonal array
Authors: Pervin Rumiya , Goswami Luna , Vijayabaskar V. , Bandyopadhyay Abhijit 
Published: June 2012

AbstractProcess parameters of poly (ethylene‐co‐vinyl acetate) (EVA)‐modified poly (ethylene‐co‐1‐octene) (POE)‐interpenetrating, double network blend was designed through Taguchi L9 orthogonal array as a novel approach for complete optimization of engineering and solvent‐swelling properties. Influence of different factors like EVA and peroxide concentrations, blending temperature, and blending time on gel content, tensile modulus, tensile strength, ultimate elongation were statistically calculated. Results showed good correlation between mathematical and physical inferences. Stress relaxation, hysteresis and other physico‐mechanicals like total elongation, solvent‐swelling, etc., were interestingly depended upon the nature of dominantly crosslinked phase instead of net crosslinking of the network hybrids. Sorption, on the other hand, depended on the hydrophobic‐hydrophilic property of the surfaces. The series of data produced finally helped to select the best process parameters under which a particular POE‐EVA blend composition yielded most balanced physico‐mechanicals. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
α‐cyclodextrin assisted self‐assembly of poly(ethylene glycol)‐block‐poly(N‐isopropylacrylamide) in aqueous media
Authors: Yuen Fanny , Tam Kam Chiu 
Published: June 2012

AbstractPoly(ethylene glycol)‐block‐poly(N‐isopropylacrylamide) (PEG‐b‐PNIPAM) block copolymers were synthesized by atom transfer radical polymerization, and the α‐cyclodextrin (α‐CD) induced self‐assembly characteristics of the system were elucidated. Below the lower critical solution temperature (LCST) of PNIPAM, CD threaded onto the PEG segments and induced micellization to form rod‐shaped nanostructures comprising of a PEG/α‐CD condensed phase and a PNIPAM shell. Increasing the temperature of system above the LCST caused the PNIPAM segments to collapse, which resulted in the dethreading of the CD. © 2012 Wiley Periodicals, Inc. J. Appl. Polym. Sci., 2013
Population prioritization for conservation of imperilled warmwater fishes in an arid‐region drainage
Authors: Clarkson Robert W. , Marsh Paul C. , Dowling Thomas E. 
Published: June 2012

ABSTRACT

Continuing reductions in biodiversity, coupled with limited resources, have generated the need to perform triage to maximize success in conservation efforts.
The development and implementation of a species recovery programme requires identifying which population units are most vulnerable to extirpation, which are most valuable to conserve, and which are most feasibly restored.
Based on a review of the recent literature, there are few generalized approaches available to guide such prioritization procedures, but to adapt one existing method was adapted to rank populations of six imperilled, native, warmwater fish species of the Gila River basin of Arizona‐New Mexico, USA and Sonora, Mexico.
The approach uses a series of binary questions that consider the biological consequences of extinction and whose scores accumulate to rank conservation value.
With significant adjustment, the approach accommodated differences among species, evolutionary ecologies, habitats, and conservation problems between Pacific salmon stocks (the basis of the original method) and desert fishes of the arid American Southwest.
A major result of the population prioritization process was that some of the highest‐ranked populations have poor recovery potential, and challenges to recovery are daunting.
Isolation management (segregation of native from non‐native fishes) is considered the primary recovery action for the native fauna.
The general method appears adaptable for application to other conservation situations and taxa, but its value will be realized only if it results in implementation of ‘in‐the‐water’ recovery actions. Copyright © 2012 John Wiley & Sons, Ltd.
T‐optimality and neural networks: a comparison of approaches for building experimental designs
Authors: Berni Rossella , De March Davide , Stefanini Federico M. 
Published: June 2012

This paper deals with optimal experimental design criteria and neural networks in the aim of building experimental designs from observational data. It addresses the following three main issues: (i) the introduction of two radically different approaches, namely T‐optimal designs extended to Generalized Linear Models and Evolutionary Neural Networks Design; (ii) the proposal of two algorithms, based on model selection procedures, to exploit the information of already collected data; and (iii) the comparison of the suggested methods and corresponding algorithms by means of a simulated case study in the technological field.Results are compared by considering elements of the proposed algorithms, in terms of models and experimental design strategies. In particular, we highlight the algorithmic features, the performances of the approaches, the optimal solutions and the optimal levels of variables involved in a simulated foaming process. The optimal solutions obtained by the two proposed algorithms are very similar, nevertheless, the differences between the paths followed by the two algorithms to reach optimal values are substantial, as detailed step‐by‐step in the discussion. Copyright © 2012 John Wiley & Sons, Ltd.
Numerical evaluation of currents induced in a worker by ELF non‐uniform electric fields in high voltage substations and comparison with experimental results
Authors: Tarao Hiroo , Korpinen Leena H. , Kuisti Harri A. , Hayashi Noriyuki , Elovaara Jarmo A. , Isaka Katsuo 
Published: June 2012

AbstractAn ungrounded human, such as a substation worker, receives contact currents when touching a grounded object in electric fields. In this article, contact currents and internal electric fields induced in the human when exposed to non‐uniform electric fields at 50 Hz are numerically calculated. This is done using a realistic human model standing at a distance of 0.1–0.5 m from the grounded conductive object. We found that the relationship between the external electric field strength and the contact current obtained by calculation is in good agreement with previous measurements. Calculated results show that the contact currents largely depend on the distance, and that the induced electric fields in the tissues are proportional to the contact current regardless of the non‐uniformity of the external electric field. Therefore, it is concluded that the contact current, rather than the spatial average of the external electric field, is more suitable for evaluating electric field dosimetry of tissues. The maximum induced electric field appears in the spinal cord in the central nervous system tissues, with the induced electric field in the spinal cord approaching the basic restriction (100 mV/m) of the new 2010 International Commission on Non‐Ionizing Radiation Protection guidelines for occupational exposure, if the contact current is 0.5 mA. Bioelectromagnetics 34:61–73, 2013. © 2012 Wiley Periodicals, Inc.
Global regulator engineering significantly improved Escherichia coli tolerances toward inhibitors of lignocellulosic hydrolysates
Authors: Wang Jianqing , Zhang Yan , Chen Yilu , Lin Min , Lin Zhanglin 
Published: June 2012

AbstractLignocellulosic biomass is regarded as the most viable source of feedstock for industrial biorefinery, but the harmful inhibitors generated from the indispensable pretreatments prior to fermentation remain a daunting technical hurdle. Using an exogenous regulator, irrE, from the radiation‐resistant Deinococcus radiodurans, we previously showed that a novel global regulator engineering (GRE) approach significantly enhanced tolerances of Escherichia coli to alcohol and acetate stresses. In this work, an irrE library was subjected to selection under various stresses of furfural, a typical hydrolysate inhibitor. Three furfural tolerant irrE mutants including F1‐37 and F2‐1 were successfully obtained. The cells containing these mutants reached OD600 levels of 4‐ to 16‐fold of that for the pMD18T cells in growth assay under 0.2% (v/v) furfural stress. The cells containing irrE F1‐37 and F2‐1 also showed considerably reduced intracellular oxygen species (ROS) levels under furfural stress. Moreover, these two irrE mutants were subsequently found to confer significant cross tolerances to two other most common inhibitors, 5‐hydroxymethyl‐2‐furaldehyde (HMF), vanillin, as well as real lignocellulosic hydrolysates. When evaluated in Luria–Bertani (LB) medium supplemented with corn stover cellulosic hydrolysate (prepared with a solid loading of 30%), the cells containing the mutants exhibited lag phases markedly shortened by 24–44 h in comparison with the control cells. This work thus presents a promising step forward to resolve the inhibitor problem for E. coli. From the view of synthetic biology, irrE can be considered as an evolvable “part” for various stresses. Furthermore, this GRE approach can be extended to exploit other exogenous global regulators from extremophiles, and the native counterparts in E. coli, for eliciting industrially useful phenotypes. Biotechnol. Bioeng. 2012; 109: 3133–3142. © 2012 Wiley Periodicals, Inc.
Improved RP‐HPLC method for determination of bovine lactoferrin and its proteolytic degradation in simulated gastrointestinal fluids
Authors: Yao Xudong , Bunt Craig , Cornish Jillian , Quek Siew‐Young , Wen Jingyuan 
Published: June 2012

ABSTRACTThe objective of this study was to qualitatively and quantitatively evaluate bovine lactoferrin (bLf) and its stability using a rapid RP‐HPLC method. bLf could be rapidly detected within 20 min and quantitated at levels down to 5 µg/mL, and the equation of linearity was y = 86.10x + 178.31 with the correlation coefficient (r2) 0.9997. Quantitative data obtained in the present study proved the improved RP‐HPLC method to be a sensitive and accurate analytical tool for bLf determination. The proteolytic cleavage of bLf in simulated human gastrointestinal fluids was further analyzed by RP‐HPLC, and found to follow pseudo‐first‐order kinetics. The typical equation obtained by pepsin was log10 [At]/[A0] = −0.03x (r2 = 0.85), and log10 [At]/[A0] = −0.01x (r2 = 0.81) for trypsin and chymotrypsin combination. Pepsinolysis of bLf in simulated gastric fluid was relatively fast with the half‐life t1/2 23.1 min. The digestion of bLf in simulated intestinal fluid was slower with about a 3‐fold increase in half‐life (69.3 min). After the complete proteolysis of bLf, small cleaved peptide fragments were fully separated and identified by RP‐HPLC. The proteolytic study indicated that this validated RP‐HPLC was able to evaluate bLf stability though monitoring the derivatization products. Copyright © 2012 John Wiley & Sons, Ltd.
Overexpression of Snail induces epithelial–mesenchymal transition and a cancer stem cell–like phenotype in human colorectal cancer cells
Authors: Fan Fan , Samuel Shaija , Evans Kurt W. , Lu Jia , Xia Ling , Zhou Yunfei , Sceusi Eric , Tozzi Federico , Ye Xiang‐Cang , Mani Sendurai A. , Ellis Lee M. 
Published: June 2012

AbstractEpithelial–mesenchymal transition (EMT) is a critical process providing tumor cells with the ability to migrate and escape from the primary tumor and metastasize to distant sites. Recently, EMT was shown to be associated with the cancer stem cell (CSC) phenotype in breast cancer. Snail is a transcription factor that mediates EMT in a number of tumor types, including colorectal cancer (CRC). Our study was done to determine the role of Snail in mediating EMT and CSC function in CRC. Human CRC specimens were stained for Snail expression, and human CRC cell lines were transduced with a retroviral Snail construct or vector control. Cell proliferation and chemosensitivity to oxaliplatin of the infected cells were determined by the MTT (colorimetric 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide) assay. Migration and invasion were determined in vitro using modified Boyden chamber assays. EMT and putative CSC markers were analyzed using Western blotting. Intravenous injection of tumor cells was done to evaluate their metastatic potential in mice. Snail was overexpressed in human CRC surgical specimens. This overexpression induced EMT and a CSC‐like phenotype in human CRC cells and enhanced cell migration and invasion (P < 0.002 vs. control). Snail overexpression also led to an increase in metastasis formation in vivo (P < 0.002 vs. control). Furthermore, the Snail‐overexpressing CRC cells were more chemoresistant to oxaliplatin than control cells. Increased Snail expression induces EMT and the CSC‐like phenotype in CRC cells, which enhance cancer cell invasion and chemoresistance. Thus, Snail is a potential therapeutic target in metastatic CRC.
ChemInform Abstract: Single‐Step Synthesis of FeSO4F1‐yOHy (0 ≤ y ≤ 1) Positive Electrodes for Li‐Based Batteries.
Authors: Ati M. , Sougrati M. T. , Rousse G. , Recham N. , Doublet M. L. , Jumas J. C. , Tarascon J. M. 
Published: June 2012

AbstractThe title phases are synthesized from ball‐milled, stoichiometric mixtures of FeF3 and Fe2(SO4)3·nH2O (autoclave, 300 °C, 50 h).
ChemInform Abstract: Selective Two‐Step Oxidation of μ2‐S Ligands in Trigonal Prismatic Unit {Re3(μ6‐C) (μ2‐S)3Re3} of the Bioctahedral Cluster Anion [Re12CS17(CN)6]6‐.
Authors: Mironov Yuri V. , Gayfulin Yakov M. , Kozlova Svetlana G. , Smolentsev Anton I. , Tarasenko Maria S. , Nizovtsev Anton S. , Fedorov Vladimir E. 
Published: June 2012

AbstractOxidation of the cluster anion [Re12CS17(CN)6]6‐ by H2O2 in water is investigated.
ChemInform Abstract: Protecting Group Free, Stereocontrolled Synthesis of β‐Haloenamides.
Authors: Pasqua Adele E. , Crawford James J. , Long De‐Liang , Marquez Rodolfo 
Published: June 2012

AbstractA new, efficient, and stereoselective method to prepare (Z)‐ or (E)‐products is reported.
ChemInform Abstract: Enantioselective α‐Benzoyloxylation of Ketones Promoted by Primary Amine Catalyst.
Authors: Jadhav Milind S. , Righi Paolo , Marcantoni Enrico , Bencivenni Giorgio 
Published: June 2012

AbstractThe method allows the preparation of protected α‐hydroxy ketones and 1,2‐diols.
ChemInform Abstract: An Easy Stereoselective Copper(I)‐Catalyzed Synthesis of (E)‐3‐Trifluoromethylbut‐2‐en‐3‐ynoate via Palladium‐Free Stille Coupling Reaction.
Authors: Zine Khalid , Petrignet Julien , Thibonnet Jerome , Abarbri Mohamed 
Published: June 2012

AbstractIn most cases, clean formation of (E)‐configurated products is observed.
ChemInform Abstract: Cyclopentane‐1,2‐dione Bis(tert‐butyldimethylsilyl) Enol Ether in Asymmetric Organocatalytic Mukaiyama—Michael Reactions.
Authors: Reile Indrek , Paju Anne , Kanger Tonis , Jaerving Ivar , Lopp Margus 
Published: June 2012

AbstractEther (I) readily reacts with α,β‐unsaturated aldehydes to give the adducts (III) as 1,2‐diketone structural subunits with good e.e.values after reductive work‐up and deprotection (no details for step 2 and 3 accessible).
ChemInform Abstract: Copper‐Catalyzed Coupling of 1,1‐Dibromo‐1‐alkenes with Phenols: A General, Modular, and Efficient Synthesis of Ynol Ethers, Bromo Enol Ethers, and Ketene Acetals.
Authors: Jouvin Kevin , Bayle Alexandre , Legrand Frederic , Evano Gwilherm 
Published: June 2012

AbstractThe process can be controlled by changing the conditions and thus affords the different types of title compounds.
ChemInform Abstract: An Efficient and Reusable Palladium Catalyst Supported on a Rasta Resin for Suzuki—Miyaura Cross‐Couplings.
Authors: Diebold Carine , Becht Jean‐Michel , Lu Jinni , Toy Patrick H. , Le Drian Claude 
Published: June 2012

AbstractThe catalyst can be reused up to five times without loss of efficiency.