bluuebunny/crossref_metadata_2025_split huggingface dataset:
Migration of Central and East European Acrocephalus Warblers at the Eastern Adriatic Coast: An Analysis of Recoveries
Authors: Kralj Jelena , Radović Dragan , Tutiš Vesna , Ćiković Davor 
Published: July 2009

Migration of Central and East European Acrocephalus Warblers at the Eastern Adriatic Coast: An Analysis of Recoveries
Migration routes and origins of the Reed Warbler (Acrocephalus scirpaceus), Great Reed Warbler (A. arundinaceus) and Sedge Warbler (A. schoenobaenus) populations migrating through the eastern Adriatic coast were investigated by analysing recoveries of birds ringed or found at two eastern Adriatic wetlands during autumn migration. There were 75, 104 and 63 long-distance recoveries for these three species, respectively. Great Reed Warblers from central and eastern Europe and Sedge Warblers from countries surrounding the Baltic Sea use the eastern Adriatic wetlands as stopover sites and continue their migration across the Mediterranean. Some Great Reed Warblers use south-western route during their return migration. Reed Warblers of unknown origin, presumably from eastern Europe continue their migration through south-western route to Spain (mean distance - 1329.3 ± 118.0 km, n = 20; mean azimuth - 251.05 ± 4.91°, n = 20; mean velocity - 63.25 km/day, n = 16). Local breeding Reed Warblers migrate southeast along the eastern Mediterranean (azimuth 117.53°). Eastern Adriatic coast represents a crossroads for migratory warblers using south-western, south-eastern and central Mediterranean flyways.
Biometrics and Morphology Variation within Sex-Age Groups of Robins (Erithacus rubecula) Migrating through the Polish Baltic Coast
Authors: Rosińska Katarzyna 
Published: July 2009

Biometrics and Morphology Variation within Sex-Age Groups of Robins (Erithacus rubecula) Migrating through the Polish Baltic Coast
Biometrical and morphological differentiation in sex-age groups of Robins migrating through the Polish Baltic coast was studied. Altogether 446 dead birds were collected in 1963-2004 during spring or autumn migration. Dead Robins were measured (wing and tail lengths and wing formula) and additionally in 2002-2004 leg colour and amount of grey colour on head and flanks were determined. After the measurements were taken, individuals were sexed by dissection.
Birds were divided in four sex-age classes: immature females, immature males, adult females and adult males. Wing length, tail length, index of asymmetry (E') and pointedness (L) were compared among this classes using t-test. G-test was used to compare leg colour and greyness on head and flanks between the sexes for immature Robins. With a method of correlative topography charts showing combination of two parameters - wing and tail lengths - in different sex-groups of immatures were prepared.
Obtained results indicate that sexes differ in wing and tail length and greyness on head and flanks. Males have longer wings and tails and bigger amount of greyness than females. The overlap of females and males is rather large, i.e. in the case of wing length 69-75 mm in immature and 71-74 mm in adult birds and in the case of tail length 55-67 mm in immature and 57-64 mm in adult birds. High probability of sex determination according to wing or tail length is found only in extremes. Combination of these two features only slightly increases possibility of sex determination.
The Robin is a monomorphic species, therefore biometrical differences between males and females could be helpful in sexing. In some studies Robins were sexed according to criteria proposed by J. Pettersson referring to wing length, but only 29% of birds analysed here could be sexed when applying this criterion. These differences may result from varying composition of populations coming from different breeding areas. According to the charts presenting correlation between wing and tail length, three "biometrical" groups are distinguishable in both sexes.
Evaluation of Typhidot (IgM) for Early Diagnosis of Typhoid Fever
Authors: Begum Zohra , Hossain Md Akram , Shamsuzzaman AKM , Ahsan Md Monjurul , Musa AKM , Mahmud Md Chand , Sumona Asma Azad , Ahmed Salma , Jahan Nahida Akhter , Khaleque Md Abdul 
Published: July 2009

Typhoid fever still continues to be a major public health problem, particularly in many developing countries. A simple, reliable, affordable and rapid diagnostic test has been a long-felt need of the clinicians. We, therefore, prospectively evaluated the sensitivity and specificity of Typhidot (IgM), a serological test to identify IgM antibodies against Salmonella typhi. The study was carried out in the department of Microbiology, Mymensingh Medical College, Mymensingh between June, 2006 and July, 2007, on a total of 100 samples from clinically suspected patients to have typhoid fever. Blood culture as well as Typhidot test were performed for each of the cases. Out of 100 clinically diagnosed typhoid fever, 14 were blood culture positive for S. typhi and 73 were Typhidot (IgM) positive. Among 14 culture positive cases, 13 (92.85%) were Typhidot (IgM) positive. The test was also positive in 04 (20%) out of 20 febrile controls. None of the healthy controls was positive by Typhidot (IgM). The sensitivity, specificity, positive predictive value and negative predictive value of the test using blood culture as gold standard were 92.85%, 90.00%, 76.47% and 97.29% respectively for typhoid fever. Typhidot (IgM) test is rapid, easy to perform and reliable test for diagnosing typhoid fever, and useful for small, less equipped laboratories as well as for the laboratories with better facilities. Key words: Typhoid fever, Salmonella typhi, Typhidot (IgM) test doi: 10.3329/bjmm.v3i1.2964 Bangladesh J Med Microbiol 2009; 03 (01): 10-13
Seroprevalence of Hepatitis Virus Antibodies in Newly Admitted Students of Sylhet MAG Osmani Medical College
Authors: Ahmed Muhammad Shamsher , Chowdhury Osul Ahmed , Khatoon Mohsina , Kabir Fahmin , Chowdhury Ahmed Riad , Jahan Halima 
Published: July 2009

Blood samples from randomly selected 100 newly admitted first year MBBS students of Sylhet MAG Osmani Medical College were tested to find the seroprevalence of antibodies against Hepatitis A, Hepatitis B, Hepatitis C and Hepatitis E viruses. The study revealed a lower seroprevalence of HAV antibodies than the common perception of their age group. The rate of vaccination against hepatitis B virus was found not very high, considering the affluence of their parents. Also there were lack of awareness about risk factors of viral hepatitis and many of the students were in habit of taking food outside. Institutionalized vaccination policy for 1st year medical students against Hepatitis A and Hepatitis B along with awareness building measures regarding the professional risk among the new entrants are suggested. Policy planners and other stakeholders in the field should come up with a comprehensive intervention strategy to protect the future caregivers from the scourge of hepatitis viruses. Keywords: Hepatitis A, Hepatitis B, Hepatitis C, Hepatitis E, Seroprevalence doi: 10.3329/bjmm.v3i1.2967 Bangladesh J Med Microbiol 2009; 03 (01): 20-26
Radiation exposure of patients during 68Ga-DOTATOC PET/CT examinations
Authors: Zöphel K. , Freudenberg R. , Oehme L. , Andreeff M. , Wunderlich G. , Eisenhofer G. , Kotzerke J. , Hartmann H. 
Published: July 2009

Summary
Aim: Investigation of the biodistribution and calculation of dosimetry of Ga-68-DOTATOCfor patients imaged in the routine clinical setting for diagnosis or exclusion of neuroendocrine tumours. Patients, methods: Dynamic PET/CT-imaging (Biograph 16) was performed over 20 min in 14 patients (8 men, 6 women) after injection of (112 ± 22) MBq 68Ga-DOTATOC followed by whole body 3D-acquisition (8 bed positions, 3 or 4 min each) 30 min p.i. and 120 min p.i. Urinary tracer elimination was measured and blood activity was derived non-invasively from the blood pool of the heart. The relevant organs for dosimetry were spleen, kidneys, liver, adrenals, urinary bladder and pituitary gland. Dosimetry was performed using OLINDA/ EXM 1.0 software and specific organ uptake was expressed as standardized uptake values (SUVs). Results: Rapid physiological uptake of the radiotracer could be demonstrated in liver, spleen and kidneys, adrenals and pituitary gland (mean SUVs were 6, 20, 16, 10, and 4, respectively). Radiotracer elimination was exclusively via urine (16% of injected dose within 2h); no redistribution could be observed. The spleen and the kidneys received the highest radiation exposure (0.24 mSv/MBq, 0.22 mSv/MBq resp.), mean effective dose yielded 0.023 mSv/MBq. Conclusion: 68Ga-DOTATOC is used extensively for diagnosis of somatostatin receptor positive tumours because it has several advantages over the 111In-labelled ligand. The derived dosimetric values are lower than first approximations from the biological data of OctreoScan. The use of CT for transmission correction of the PET data delivers radiation exposure up to 1 mSv (low dose).
Selection and mass spectrometry characterization of peptides targeting semiconductor surfaces
Authors: Estephan Elias , Larroque Christian , Bec Nicole , Martineau Pierre , Cuisinier Frédéric J.G. , Cloitre Thierry , Gergely Csilla 
Published: July 2009

AbstractWe report on elaboration of 12‐mer peptides that reveal specific recognition for the following semiconductor (SC) surfaces: GaAs(100), InAs(100), GaN(0001), ZnSe(100), ZnTe(100), GaAs(111)A, GaSb(100), CdSe(100). A M13 bacteriophage library was used to screen 109 different 12‐mer peptides against these substrates to finally isolate, in maximum six amplification cycles, peptides that bind to the target surfaces. The specific peptides for the InAs and ZnSe surfaces were obtained. Contrary, for the other SC surfaces several peptides with high affinities have been isolated. Aiming for a better specificity, when the phage display has been conducted through six cycles, the screening procedure got dominated by a phage present in the M13 bacteriophage library and the SVSVGMKPSPRP peptide has been selected for different SCs. The high amplification potential of this phage has been observed previously with different targets. Thus, precaution should be undertaken in defining adhesion peptides with the phage display technique and real affinity of the obtained biolinkers should be studied with other methods. We employed mass spectrometry (MALDI‐TOF/TOF) to demonstrate the preferential attachment (or not) of the SVSVGMKPSPRP peptide to the different SC surfaces. This allows us to define a realistic selection of the expressed peptides presenting affinity for the studied eight SC surfaces. We demonstrate that with increasing the dielectric constants of the employed solvents, adhesion of the SVSVGMKPSPRP peptide onto GaN(0001) is hindered. Biotechnol. Bioeng. 2009; 104: 1121–1131. © 2009 Wiley Periodicals, Inc.
Quantitative characterization of quantum dot‐labeled lambda phage for Escherichia coli detection
Authors: Yim Peter B. , Clarke Matthew L. , McKinstry Michael , De Paoli Lacerda Silvia H. , Pease Leonard F. , Dobrovolskaia Marina A. , Kang HyeongGon , Read Timothy D. , Sozhamannan Shanmuga , Hwang Jeeseong 
Published: July 2009

AbstractWe characterize CdSe/ZnS quantum dot (QD) binding to genetically modified bacteriophage as a model for bacterial detection. Interactions among QDs, lambda (λ) phage, and Escherichia coli are examined by several cross‐validated methods. Flow and image‐based cytometry clarify fluorescent labeling of bacteria, with image‐based cytometry additionally reporting the number of decorated phage bound to cells. Transmission electron microscopy, image‐based cytometry, and electrospray differential mobility analysis allow quantization of QDs attached to each phage (4–17 QDs) and show that λ phage used in this study exhibits enhanced QD binding to the capsid by nearly a factor of four compared to bacteriophage T7. Additionally, the characterization methodology presented can be applied to the quantitative characterization of other fluorescent nanocrystal‐biological conjugates. Biotechnol. Bioeng. 2009;104: 1059–1067. Published 2009 Wiley Periodicals, Inc.
Vascular endothelial growth factor in the management of hepatocellular carcinoma
Authors: Kaseb Ahmed O. , Hanbali Amr , Cotant Matthew , Hassan Manal M. , Wollner Ira , Philip Philip A. 
Published: July 2009

AbstractThe importance of tumor angiogenesis in tumor biology is now widely accepted. Hepatocellular carcinoma (HCC) is a highly vascular tumor, and angiogenesis is believed to play a considerable role in its development and progression. The authors reviewed the role of circulating vascular endothelial growth factor (VEGF) in screening for HCC and in risk stratification and treatment monitoring. They searched the world medical literature by accessing MEDLINE and PubMed for articles on: 1) the utility of circulating VEGF for HCC screening in patients with cirrhosis; 2) the role of circulating VEGF as a predictor of the invasive potential of HCC; and 3) monitoring anti‐HCC treatment effects by serial measurements of circulating VEGF. They found evidence to support a potential role for VEGF in screening and surveillance of HCC. They also found support for developing the use of VEGF in the monitoring of treatment outcomes. Several studies suggested that the circulating VEGF level may be an independent prognostic marker in HCC. Further studies are needed to determine the utility of circulating VEGF in screening of patients with cirrhosis and to determine its potential role as a prognostic and predictive biomarker in patients with HCC. Cancer 2009. © 2009 American Cancer Society.
Protein composition of liver cyst fluid from the BALB/c‐cpk/+ mouse model of autosomal recessive polycystic kidney disease
Authors: Lai Xianyin , Blazer‐Yost Bonnie L. , Gattone Vincent H. , Muchatuta Monalisa N. , Witzmann Frank A. 
Published: July 2009

AbstractCysts arising from hepatic bile ducts are a common extra‐renal pathology associated with polycystic kidney disease in humans. As an initial step in identifying active components that could contribute to disease progression, we have investigated the protein composition of hepatic cyst fluid in an orthologous animal model of autosomal recessive polycystic kidney disease, heterozygous (BALB/c‐cpk/+) mice. Proteomic analysis of cyst fluid tryptic digests using LC‐MS/MS identified 303 proteins, many of which are consistent with enhanced inflammatory cell processes, cellular proliferation, and basal laminar fibrosis associated with the development of hepatic bile duct cysts. Protein identifications have been submitted to the PRIDE database (http://www.ebi.ac.uk/pride), accession number 9227.
LymPHOS: Design of a phosphosite database of primary human T cells
Authors: Ovelleiro David , Carrascal Montserrat , Casas Vanessa , Abian Joaquin 
Published: July 2009

AbstractCurrent proteomic technology is capable of producing huge amounts of analytical information, which is often difficult to manage in a comprehensive form. Curation, further annotation and public communication of proteomic data require the development of standard data formats and efficient, multimedia database structures. We have implemented a workflow for the annotation of a phosphopeptide database (LymPHOS) that includes tools for MS data filtering and phosphosite assignation, mass spectrum visualization, experimental description and accurate phosphorylation site assignation. Experimental annotations were fitted to current minimum information about a proteomics experiment guidelines. A new guideline for phosphoprotein sample preparation is also proposed. Currently, the database describes 342 phosphorylation sites mapping to more than 200 gene sequences, and it can be accessed through the net (http://www.lymphos.org).
Quantitative proteomic analysis of lentiviral vectors using 2‐DE
Authors: Denard Jérôme , Rundwasser Stéphanie , Laroudie Nicolas , Gonnet Florence , Naldini Luigi , Radrizzani Marina , Galy Anne , Merten Otto‐Wilhelm , Danos Olivier , Svinartchouk Fedor 
Published: July 2009

AbstractAmong the integrative gene therapy vectors developed to date, human immunodeficiency virus type 1 (HIV‐1)‐derived lentiviral vectors (LV) are distinguished by their capacity to infect both dividing and non‐dividing cells. Recombinant LV particles contain viral proteins necessary for their packaging, infectious and integrating functions. Like the parental HIV‐1 virus they are able to acquire various cellular proteins, but the number and localisation of these proteins are poorly characterised. In the present study we used 2‐DE followed by MALDI‐TOF to quantify the protein content of several types of vesicular stomatitis virus G‐pseudotyped LV including those that were extensively purified in the perspective of clinical gene therapy studies. A proteinase K treatment was used to distinguish between cellular proteins incorporated into virions (I‐proteins) and those co‐purified with vectors (C‐proteins). We found 10 C‐proteins and 18 I‐proteins associated with LV. Copy numbers for these core I‐proteins varied from 5 (AIP‐1/ALIX) to 280 (Cyclophilin A) per vector particle. Three novel I‐proteins, guanine nucleotide‐binding protein 2, L‐lactate dehydrogenase B chain and hnRNP core protein A1, were found. This study defines for the first time, the protein stoichiometry of infectious HIV‐1‐derived LV particles.
Liquid Chromatography–High‐Resolution Mass Spectrometry for Quantitative Analysis of Gangliosides
Authors: Fong Bertram , Norris Carmen , Lowe Edwin , McJarrow Paul 
Published: July 2009

AbstractGangliosides are a large family of glycosphingolipids that are abundant in the brain, and have been shown to affect neuronal plasticity during development, adulthood, and aging. We developed a fast, efficient, and sensitive liquid chromatography–mass spectrometry method to quantify eight different classes of gangliosides (GM1, GM2, GM3, GD3, GD1a, GD1b, GT1b, GQ1b) in the brains of 2‐day‐old and 80‐day‐old Wistar rats. The gangliosides were extracted from rat brain using a modified Svennerholm and Fredman method. After ganglioside class separation using a hydrophilic high performance liquid chromatography (HPLC) column, the resolving power of the LTQ‐Orbitrap™ mass spectrometer was used to extract and sum the major species of each ganglioside class, generating fully resolved extracted ion current peaks for both standards and samples. The flexibility and the specificity of this method are such that it can be applied to the analysis of other ganglioside species/classes not discussed in this paper, provided appropriate standards are available. The method had good repeatability (coefficient of variation 4.8–12.3%) and mean recoveries in the range 92–107%.
Are high school students living in lodgings at an increased risk for internalizing problems?
Authors: Wannebo Wenche , Wichstr⊘m Lars 
Published: July 2009

ABSTRACTThis study aimed to investigate whether leaving home to live in lodgings during senior high school can be a risk factor for the development of internalizing problems. Utilizing two large‐scale prospective community studies of 2399 and 3906 Norwegian students (age range 15–19 years), respectively, the difference in internalizing symptoms between adolescents living in lodgings and adolescents living with their parents during senior high school was examined. Female students living in lodgings had higher scores on internalizing problems than female students living at home, whereas no differences were found for males. Living in lodgings did not predict later internalizing problems, and prior internalizing problems did not predict moving into lodgings. It is therefore suggested that the negative effect of living in lodgings on high school students' well‐being is temporary.
Abnormal Antenatal Sonogram: An Indicator of Disease Severity in Children with Posterior Urethral Valves
Authors: Harvie Sarah , McLeod Lynne , Acott Philip , Walsh Eilish , Abdolell Mohamed , Macken Marian B. 
Published: July 2009

Purpose To review the association of an abnormal prenatal sonogram with most recent serum creatinine in patients with proven posterior urethral valves (PUV). Methods Since 1992, all live-born patients between 1992–2004 with clinically proven PUV, with postnatally proven PUV, from 2 pediatric tertiary care centers, were reviewed for age at diagnosis, most recent serum creatinine, presence of chronic renal failure (CRF) (serum creatinine >2 standard deviations above normal for age), or end stage renal disease (dialysis or transplant). Available antenatal reports from the 2 centres and surrounding community hospitals were reviewed for gestational age (GA) at the time of ultrasound, volume of amniotic fluid, and urinary-tract abnormality. Results Thirty-four patients with proven PUV and prenatal sonograms were identified (1992–2004). Eighteen patients had abnormalities on their prenatal sonogram, with poor outcome in 5 (mean follow-up, 8 years [1–13 y]). No specific features were identified on prenatal sonogram. Sixteen patients had normal prenatal sonograms, with poor outcomes in 2 (mean follow-up, 8 years [3–13y]). There is an increased risk of an abnormal serum creatinine among those patients with an abnormal prenatal study, odds ratio (OR) 2.6 (95% confidence interval, 0.35–32). Conclusions PUV represents a spectrum of disease severity. A normal prenatal ultrasound does not preclude PUV. The majority of patients with a normal prenatal examination have good outcomes. The OR suggests that there may be increased risk for poor outcome in those with an abnormal prenatal examination. A multicenter study is necessary to obtain a larger sample size and more precise ORs.
Too much theory, not enough understanding⋆
Authors: Schmenner Roger W. , Van Wassenhove Luk , Ketokivi Mikko , Heyl Jeff , Lusch Robert F. 
Published: July 2009

AbstractThis essay and the following commentaries address the use of theory in operations management. While much is said about theory in the typical journal article, theory, as science defines it, is not at the center of much of our research. The discipline had fallen into some bad habits. This essay and its commentaries appeal for more attention to what theory can mean for our understanding of operations management.
Stable isotope and 14C study of biogenic calcrete in a termite mound, Western Cape, South Africa, and its palaeoenvironmental significance
Authors: Potts Alastair J. , Midgley Jeremy J. , Harris Chris 
Published: July 2009

AbstractLate Quaternary terrestrial climate records from the semi-arid zone of the Western Cape of South Africa are rare. However, palaeoenvironmental information may be inferred from ancient termite mounds of the region. Calcrete lenses in these mounds have δ13C and δ18O values that show systematic changes with radiocarbon dates, which range from 33,629–36,709 to 21,676–23,256 cal yr BP. These dates confirm that these heuweltjies had been present in the landscape since the last glacial period. The decrease in δ13C and δ18O from 33,629–36,709 to 21,676–23,256 cal yr BP indicates that climate information is recorded by the calcretes. It is suggested that a progressive decline in air temperature and an increase in moisture availability, and a decline in abundance of C4 or CAM plants, occurred in the region during the time heuweltjie calcite precipitated.