bluuebunny/crossref_metadata_2025_split huggingface dataset:
Histone H3K79 methyltransferase Dot1L is directly activated by thyroid hormone receptor during Xenopus metamorphosis
Authors: Matsuura Kazuo , Fujimoto Kenta , Das Biswajit , Fu Liezhen , Lu Christopher D , Shi Yun-Bo 
Published: July 2012

Abstract

Background
Thyroid hormone (T3) is important for adult organ function and vertebrate development. Amphibian metamorphosis is totally dependent on T3 and offers a unique opportunity to study how T3 controls postembryonic development in vertebrates. Earlier studies have demonstrated that TR mediates the metamorphic effects of T3 in Xenopus laevis. Liganded TR recruits histone modifying coactivator complexes to target genes during metamorphosis. This leads to nucleosomal removal and histone modifications, including methylation of histone H3 lysine (K) 79, in the promoter regions, and the activation of T3-inducible genes.

Results
We show that Dot1L, the only histone methyltransferase capable of methylating H3K79, is directly regulated by TR via binding to a T3 response element in the promoter region during metamorphosis in Xenopus tropicalis, a highly related species of Xenopus laevis. We further show that Dot1L expression in both the intestine and tail correlates with the transformation of the organs.

Conclusions
Our findings suggest that TR activates Dot1L, which in turn participates in metamorphosis through a positive feedback to enhance H3K79 methylation and gene activation by liganded TR.
Two new aliphatic lactones from the fruits of Coriandrum sativum L.
Authors: Naquvi Kamran J , Ali Mohammed , Ahmad Javed 
Published: July 2012

Abstract

Background
The present paper describes the isolation and characterization of two new aliphatic δ-lactones along with three glycerides and n-nonadecanyl cetoleate from the fruits of Coriandrum sativum L. (Apiaceae). The structures of all the isolated phytoconstituents have been established on the basis of spectral data analysis and chemical reactions.

Results
Phytochemical investigation of the methanolic extract of C. sativum L. (Apiaceae) fruits resulted in the isolation of two new aliphatic δ-lactones characterized as 2α-n-heptatriacont-(Z)-3-en-1,5-olide (1) (coriander lactone) and 2α-n-tetracont-(Z,Z)-3,26-dien-18α-ol-1,5-olide (2) (hydroxy coriander lactone) together with glyceryl-1,2-dioctadec-9,12-dienoate-3-octadec-9-enoate (3); glyceryl-1,2,3-trioctadecanoate (4); n-nonadecanyl-n-docos-11-enoate (5) and oleiyl glucoside (6).

Conclusions
Phytochemical investigation of the methanolic extract of C. sativum gave coriander lactone and hydroxy coriander lactone as the new phytoconstituents.
Use of Guided Tissue Regeneration in the Treatment of a Severe Endodontic–Periodontic Lesion: A 15‐Year Follow‐Up Case Report
Authors: Santana Ronaldo B. , Mattos Santana Carolina M. 
Published: July 2012

Introduction: Treatment of combined endodontic–periodontic lesions remains a considerable challenge in clinical practice. The degree of success in the management of these lesions is related to the efficacy of both periodontal and endodontic treatments and is influenced by specific anatomic characteristics of the lesion. This report presents the long‐term clinical outcomes of a severe endodontic–periodontic lesion treated by guided tissue regeneration (GTR) and documented over a 15‐year period.Case Presentation: A non‐smoking, 42‐year‐old female presented with a combined endodontic–periodontic lesion consisting of a large through‐and‐through apical lesion communicating with the marginal periodontium as a result of the complete loss of the facial bone plate. Treatment consisted of GTR and grafting with absorbable hydroxyapatite. The clinical variables evaluated were plaque, bleeding on probing (BOP), gingival recession, probing depth (PD), and clinical attachment level (CAL). Reevaluation was performed 1 year and 15 years after the surgical procedure. Healing was uneventful. Measurements revealed that PD was reduced by 17 mm, a 16‐mm CAL gain was recorded, and no BOP was detected at any tooth aspect. CAL gains were maintained up to the 15‐year recall. Radiographic evaluation demonstrated a complete resolution of the bony lesion at both 1 year and 15 years after surgery.Conclusion: Severe combined endodontic–periodontic lesions can be successfully treated via regenerative techniques, and the resultant bone and CAL gains are preserved for several years after the active treatment.
A Case Report on Combination Therapy Using a Composite Allograft Containing Mesenchymal Cells With an Amnion–Chorion Barrier to Treat a Mandibular Class III Furcation
Authors: Rosen Paul S. 
Published: July 2012

Introduction: Maintaining molars with Class III furcation invasion remains one of the greatest challenges in periodontics. Surgical treatment of these areas by any means has been disappointing. This case report describes a successful combined regenerative approach using a composite allograft with mesenchymal stem cells along with a barrier derived from human amnion–chorion to close a Class III furcation.Case Presentation: The patient was a 62‐year‐old white male referred for the treatment of his periodontal and dental implant needs. The mandibular left required functional crown lengthening for the two premolars along with management of the Class III furcation on the first molar. The unopposed second molar with both an advanced osseous lesion and 1+ degree mobility was to be extracted. Surgical exposure allowed the furcation to be thoroughly scaled and planed, treated with tetracycline for root modification, followed by intramarrow penetration and recombinant platelet‐derived growth factor applied to the roots. A composite allograft containing mesenchymal cells was used to fill the defect, and it was layered by a barrier derived from human amnion–chorion tissue. The flaps were coronally advanced and sutured with expanded polytetrafluoroethylene. Six months after the completion of surgery, the furcation was closed to a Nabers probe from both the buccal and lingual aspects and radiographically, the area was radiopaque suggestive of bone fill/containment.Conclusion: This case report demonstrates the closure of a Class III furcation based on horizontal probing and radiographic bone fill, giving hope that this combined approach for regeneration may hold promise for treating this most challenging of clinical dilemmas.
Once-Weekly Glucose-Lowering Therapy for Type 2 Diabetes
Authors: LaRue Susan , DeYoung Mary Beth , Blickensderfer Amy , Chen Steve 
Published: July 2012

IN BRIEF
Exenatide once weekly (QW), a glucagon-like peptide-1 receptor agonist, is the first and only glucose-lowering therapy approved for once-weekly administration to patients with type 2 diabetes. In clinical trials, significant reductions in A1C (−1.3 to −1.9%) and weight (−2 to −4 kg) were observed over 6 months with minimal risk of hypoglycemia in the absence of a sulfonylurea. Although the risk of injection-site reactions and mild to moderate gastrointestinal events increased after initiation of exenatide QW, tolerability improved over time. Reductions in A1C and weight were maintained for 3 years in patients who continued to use exenatide QW.
A new piecewise-quasilinearization method for solving chaotic systems of initial value problems
Authors: Motsa Sandile 
Published: July 2012

Abstract
In this paper, a modification of the successive linearization method (SLM) for solving nonlinear initial value problems is introduced for the first time. The proposed method is based on a novel technique of extending the standard SLM and adapting it to a sequence of multiple intervals. In this new application the method is referred to as the piecewise successive linearization method(PSLM). This new algorithm is applied to chaotic and non-chaotic differential equations that model the Lotka-Volterra, Lorenz, Rössler and Genesio-Tesi systems. A comparative study between the new algorithm and the MATLAB Runge-Kutta based in-built solver (ode45) method is presented. The results demonstrate accuracy and reliability of the proposed PSLM algorithm.
Immobilization in biotechnology and biorecognition: from macro- to nanoscale systems
Authors: Bučko Marek , Mislovičová Danica , Nahálka Jozef , Vikartovská Alica , Šefčovičová Jana , Katrlík Jaroslav , Tkáč Ján , Gemeiner Peter , Lacík Igor , Štefuca Vladimír , Polakovič Milan , Rosenberg Michal , Rebroš Martin , Šmogrovičová Daniela , Švitel Juraj 
Published: July 2012

AbstractBiological molecules such as enzymes, cells, antibodies, lectins, peptide aptamers, and cellular components in an immobilized form are extensively used in biotechnology, in biorecognition and in many medicinal applications. This review provides a comprehensive summary of the developments in new immobilization materials, techniques, and their practical applications previously developed by the authors. A detailed overview of several immobilization materials and technologies is given here, including bead cellulose, encapsulation in ionotropic gels and polyelectrolyte complexes, and various immobilization protocols applied onto surfaces. In addition, the review summarises the screening and design of an immobilization protocol, practical applications of immobilized biocatalysts in the industrial production of metabolites, monitoring, and control of fermentation processes, preparation of electrochemical/optical biosensors and biofuel cells.
Crossing Disciplinary Borders: Perspectives on Learning About Sustainable Development
Authors: Dimenäs Jörgen , Alexandersson Mikael 
Published: July 2012

Crossing Disciplinary Borders: Perspectives on Learning About Sustainable DevelopmentWith regard to education, traditional environmentally-related issues have been intertwined with courses in natural sciences, which could entail opportunities as well as difficulties. The study concerns two knowledge matters that are usually divided into two different subject traditions - water and justice. In this article, we focus on the way teachers consider instruction within the frameworks of these two discourses and how teaching is related to sustainable development. The findings suggest that water and justice are two examples that are suitable for the problematisation of sustainable development with respect to holistic education. Current educational policies in Sweden advocate a tendency towards a more closed and subject-centred discourse, which means that the ability to successfully teach about sustainable development is made even more problematic.
Pre-Service Teachers' Use of Images in Integrating Environmental Sustainability Lessons
Authors: Muthersbaugh Debbie , Kern Anne 
Published: July 2012

Pre-Service Teachers' Use of Images in Integrating Environmental Sustainability LessonsEnvironmental sustainability is a topic widely discussed in the field of science education, yet, few entities have committed to developing environmental sustainability education standards. The Washington State Department of Education has created K-12 Integrated Environmental and Sustainability Learning Standards (IESLS, 2009), which align with current research and practices in environmental and sustainability education. This study focuses on the perceptions of secondary pre-service teachers about the use of images to teach environmental sustainability topics integrated in their content area. The research explores the question what secondary pre-service teachers' perceptions of sustainability and using images to teach environmental sustainability topics in their content area are. The participants were comprised of secondary pre-service teachers enrolled in an instructional methodology course from a small university in the Pacific Northwestern United States. Teaching environmental sustainability and integrating lessons using images had a noticeable impact on pre-service teachers' perceived future teaching practices. Participants also changed beliefs over the course of the study about using images to teach environmental sustainability topics.
Aggressiveness of Fusarium langsethiae isolates towards wheat, barley and oats in an in vitro leaf assay
Authors: Opoku Nelson , Back Matthew , Edwards Simon 
Published: July 2012

Aggressiveness of Fusarium langsethiae isolates towards wheat, barley and oats in an in vitro leaf assay
Fusarium langsethiae has been identified as the primary producer of HT-2 and T-2 in European cereals. HT-2 and T-2 are considered as two of the most potent trichothecenes mycotoxins and a public health concern in Europe. There is currently no legislation on HT-2 and T-2, however, there is a discussion limit of 500 μg kg-1 in unprocessed oats, 200 μg kg-1 for oat products and 50 μg kg-1 for infant food. There are limited data regarding F. langsethiae's pathogenicity and mycotoxin production, but it is evident that its behaviour deviates from traditional trichothecene-producing Fusarium species. This experiment was aimed at assessing the aggressiveness (measured by lesion length) of 20 different F. langsethiae isolates on wheat, barley and oats using an in vitro detached leaf assay. There was a significant (P<0.001) difference between lesion lengths formed by different F. langsethiae isolates used. Isolate Fl/2004/17(a) caused the shortest lesion on all cereals and this was significantly (P<0.001) different from that caused by isolate Fl/0/08/009/1 which caused the longest lesion on all cereals used. A highly significant difference (P<0.001) was also observed between lesions on the different cereals (wheat, barley and oats). Lesions on oats were the longest, followed by barley with wheat showing the shortest lesions.
The effects of fungicides on Fusarium spp. and their associated mycotoxins in naturally infected winter wheat grain
Authors: Suproniene Skaidre , Mankeviciene Audrone , Gaurilcikiene Irena 
Published: July 2012

The effects of fungicides on Fusarium spp. and their associated mycotoxins in naturally infected winter wheat grain
Field trials conducted at the Institute of Agriculture, Lithuanian Research Centre for Agriculture and Forestry (central part of Lithuania) in 2009 were aimed to evaluate the effect of fungicides on Fusarium Head Blight (FHB) in a naturally infected field. A single application of dimoxystrobin + epoxiconazole (Swing Gold), prothioconazole (Proline), metconazole (Juventus), tebuconazole (Folicur), prothioconazole + tebuconazole (Prosaro) was applied to winter wheat cv. ‘Zentos’ at the manufacturer's recommended doses at anthesis (BBCH 65). The FHB incidence and severity were assessed at milk and hard maturity stages. The percentage of Fusarium infected grain and deoxynivalenol (DON), zearalenone (ZEN) and T-2 toxin (T-2) concentrations in harvested grain were determined. In all fungicide treated plots a significant reduction of FHB incidence and severity was determined; however the fungicides did not exert any effect on the amount of Fusarium-infected grain as compared with the untreated control. A reduction of DON, ZEN and T-2 contents in grain was determined in tebuconazole treatments. Fusarium avenaceum (Fr.) Sacc, F. culmorum (W. G. Sm.) Sacc., F. poae (Peck) Wollenw, F. sporotrichioides Sherb. and F. tricinctum (Corda) Sacc were identified in wheat grain, F. poae was prevalent.
The Reliability and Accuracy of Knee Implants Sizing Predicted by Digital Templating
Authors: Zadorožnijs Sergejs , Kalnberzs Konstantins 
Published: July 2012

Summary
Introduction. Osteoarthritis of the knee is a common and frequently symptomatic illness. Total knee replacement (TKR) has evolved as an accepted, cost-effective and efficacious treatment modality for osteoarthritis and other forms of arthritic conditions of the knee joint. Preoperative planning is an important part of the surgical procedure. The inability to accurately determine the magnification factor of the radiograph is one of the major problems in analog preoperative planning of TKR. With the use of calibration objects, the digital images can be corrected for the magnification factor.
Aim of the Study. We aimed to determine the reliability and accuracy of digital templating in the pre-operative work-up for TKR.
Materials and Methods. A retrospective study was done in 105 caucasian adults, who had osteoarthritis of the knee. Digital templating was performed using a calibrating 25-mm metallic ball and Agfa Orthopaedic Tools digital software package by a surgeon not involved with the operation, who was blinded to the size of the implant inserted. The Press Fit Condylar Sigma Knee system was used in all the patients. Digital anteroposterior and lateral radiographs of the knee were used in measuring the implant size. The results from digital images were compared with the size of actual femoral and tibial implants used at the time of surgery.
Results. The correct size of the implant was predicted in 73 of 105 (69,5%) of the femoral and 70 of 105 (66,7%) of the tibial components. The correct size of the whole system was predicted in 58 of 105 (55,2%) cases. The digital preoperative planning predicted 104 of 105 (99,0%) femoral and tibial implants and 103 of 105 (98,1%) whole systems to within one size.
Conclusions. We conclude that digital templating using a calibrating 25-mm metallic ball and Agfa Orthopaedic Tools digital software is a reliable method of predicting the implant to within one size.
Reaction of Subcutaneous Connective Tissue of Experimental Animals on Bone Marrow Mesenchymal Stromal Cell Coated Hydroxyapatite
Authors: Zavorins Aleksejs , Pilmane Mara , Salms Girts , Skagers Andrejs , Cakstina Inese , Locs Janis 
Published: July 2012

Summary
Introduction. Hydroxyapatite (HAp) scaffolds have become an alternative to autologous bone grafts in orthopedic surgery, because it is possible to fill larger scale bone defects and of the decreased operation time and complication risks at the graft donor site, a promising method in bone regeneration is to load bone marrow - mesenchymal stromal cells (BM-MSCs) on to a HAp scaffold.
Aim of the Study. The aim of our study is to compare subcutaneous tissue reaction of experimental animals to implantation of HAp and HAp that is coated with BM-MSCs.
Materials and Methods. Autologous BM-MSCs were cultivated from crista iliaca dextra biopsy, 4 HAp ceramic implants were coated with BM-MSC and implanted in subcutaneous tissue on one side and non-coated HAp implants on the other side of the spine of 4 rabbits. Immunohistochemical staining of BM-MSC and connective tissue included TUNEL assay, NFkBp105, HSP 70k, Wnt1, TNF-α, VEGF, MMP-2 antibodies, microscoped at 400X magnification and analyzed semiquantitatively.
Results. Routine staining with haemotoxilin and eosin demonstrated formation of granulation tissue around the HAp implant, which was more distinct on the control side. There was an increased number of NFκB p105 positive cells (fibroblasts, myocytes, endotheliocytes) on the experimental side (+++), in comparison to the control side (++). HSP70 and apoptosis index was decreased on the experimental side (++ and 60%), in comparison to the control side (+++ and 70%). Number of MMP2 positive structures was increased on the control side in the fibrous capsule (++), in comparison to the experimental side, where the result varied (0 - +)
Conclusions. Cultured BM-MSC activity is partly limited due to the apoptosis, which is probably induced by environmental factors. BM-MSC coated HAp implant stimulates secretion of proliferation marker NFkBp105 in subcutaneous tissue, decreases cell stress, apoptosis and tissue degradation (decreased HSP 70, apoptotic cell count and MMP 2) to improve the local tissue quality and proving BM-MSC tissue protective effect
Pulsed Radiofrequency Effects on the Lumbar Dorsal Root Ganglion of the Domestic Porcine: Pilot Study
Authors: Arons Mihails , Pilmane Mara , Vasilevskis Edgars , Evansa Irina , Panihins Igors , Maloshik Dmitry 
Published: July 2012

SummaryIntroduction.Pulsed radiofrequency (PRF) is a percutaneous minimal invasive procedure for chronic pain management that can be used when conservative treatment methods have been ineffective. The effectiveness of PRF was demonstrated in various good quality randomized control studies, but mechanisms of action are still unclear.Aim of the Study.The aim of our study is to analyse the histological effects of PRF on the domestic porcine dorsal root ganglion (DRG), and evaluate the expression of biomarkers in gangliocytes of the subject(s).Materials and Methods.A total 3 domestic porcines were investigated. Under general anaesthesia and X-ray control, DRG PRF was performed. Four lumbar DRGs (L1, L2, L3, L4) were randomly treated. The opposite side DRGs was used as control. One month after the procedure the animal was euthanized. The lumbar region of the spine was placed in 10% formaldehyde for a month. After this fixation DRG samples were prepared for slide analysis. They were embedded in paraffin in order to obtain 3mm thick sections, which were then cut by microtome and collected on slide glasses. Using standard immunohistochemical reactions, the materials were tinted to define biomarkers neurofilaments (NF), glial fibrillary acidic protein (GFAP), heat shock protein - 70 (Hsp-70) expression and apoptosis by transferase-mediated dUTP nick-end labeling (TUNEL) analysis.Results.The number of cells with NF (26,0 ± 3,0 vs 16,1 ± 3,3; p<0,05), GFAP (12,0 ± 1,3 vs 3,2 ± 0,9; p<0,05) and Hsp-70 (10,0 ± 1,6 vs 4,2 ± 1,0; p<0,05) expression, were larger in the PRF side comparing with the control side. Additionally, glial cells in spinal ganglia of both sides demonstrated immunoreactivity. The instances of apoptosis were not significantly different, in statistical terms, between the control and experimental sides (18,0 ± 4,0 vs 20,0 ± 4,0; p=0,35).Conclusions.PRF in spinal gangliocytes of lumbar region increases neural tissue cytoskeleton factors like NF and GFAP suggesting about active regeneration processes into the cells 1 month after the procedure. Spinal gangliocytes one month after PRF treatment notably increases Hsp-70 expression suggesting about activation of cellular activity and inhibitory role reducing of oxidative stress. Similar number of apoptotic cells in spinal ganglia of lumbar region after PRF and control side suggests about inhibitory role of PRF on programmed cell death and stimulation of cell survival.
Management of Adenoid Cystic Carcinoma of Distal Trachea
Authors: Silins Ints , Spaks Artjoms , Apsvalks Maris , Lejnieks Juris Peteris , Saba Ingrida 
Published: July 2012

SummaryTracheal neoplasms occur infrequently, the majority of primary tracheal tumours in adults are malignant, and in 40% of cases it is adenoid cystic carcinoma, which can be asymptomatic for a long period of time. Surgical treatment can be justified even in advanced disease, because progression of tracheal tumour usually causes obstruction of airways. Improved long-term outcome seems to follow combined surgical resection and full-dose radiotherapy. Patients after resection of adenoid cystic carcinoma need careful and long-term observations to detect a relapse
Granular Cell Tumour of the Breast Cancer: Challenging Clinical and Radiological Mimic of Cancer
Authors: Abolins Arnis , Strumfa Ilze , Vanags Andrejs , Trofimovics Genadijs , Gardovskis Janis 
Published: July 2012

SummaryGranular cell tumours (GCT) are rare soft tissue neoplasms of unclear histogenesis affecting almost any organ or tissues. Approximately 5% of GCT cases are located in the breast. Although granular cell tumour is benign in most instances, it can mimic breast cancer by radiological and clinical traits. Increased awareness of GCT is necessary to avoid misinterpretation as cancer and subsequent overtreatment