bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hippocampal contributions to the episodic simulation of specific and general future events
Authors: Addis Donna Rose , Cheng Theresa , P. Roberts Reece , Schacter Daniel L. 
Published: September 2010

AbstractRecent studies have demonstrated that remembering past experiences and imagining future scenarios recruits a core network including the hippocampus. Even so, constructing future events engages the hippocampus more than remembering past events. This fMRI study examined whether increased hippocampal activity for future events includes both specific and general events. Participants constructed specific and general past and future events during fMRI scanning. We replicated previous findings of increased activity in the right anterior hippocampus when constructing future relative to past events, and when constructing specific relative to general events. Importantly, both effects were driven by a significant interaction between temporal direction and specificity, with specific future events resulting in more activity than other conditions, including general future events. No regions exhibited greater activity during the construction of past relative to future events, or general relative to specific events. These results suggest that the process of constructing a detailed representation of a novel and specific future event differentially engages the right anterior hippocampus compared with other forms of event simulation and recall. Future work is needed to disambiguate the role of encoding, novelty and detail recombination in engaging the right anterior hippocampus during simulation. © 2010 Wiley‐Liss, Inc.
High susceptibility to azoxymethane‐induced colorectal carcinogenesis in obese KK‐Ay mice
Authors: Teraoka Naoya , Mutoh Michihiro , Takasu Shinji , Ueno Toshiya , Nakano Katsuya , Takahashi Mami , Imai Toshio , Masuda Shuichi , Sugimura Takashi , Wakabayashi Keiji 
Published: September 2010

AbstractObesity is associated with colon carcinogenesis. However, not much information is available regarding the mechanisms of obesity‐associated colorectal cancer, and there are only few useful animal models for investigating the underlying mechanism between obesity and colorectal cancer. KK‐Ay mice exhibit severe obesity. Amount of visceral fat assessed by micro‐computed tomography was almost 15 times higher than that of same aged C57BL/6J mice. Treatment with azoxymethane (AOM; 200 μg/mouse injected once a week for 3 times) resulted in markedly increased colon aberrant crypt foci (ACF) development (≈70 ACF/mouse) in KK‐Ay mice compared with lean C57BL/6J mice (≈9 ACF/mouse). Moreover, administration of AOM at a dose of 200 μg/mouse once a week for 6 times developed colorectal adenocarcinomas within only 7 weeks after the last AOM injection. The incidence of adenocarcinoma was 88% in KK‐Ay mice and was markedly higher than the 4% observed in C57BL/6J mice. The number of tumors/mouse was 7.80 in KK‐Ay mice and also markedly higher than the 0.12 in the C57BL/6J case. Interestingly, adenocarcinomas were observed in most of the AOM‐treated KK‐Ay mice along with remarkable tumor angiogenesis, and some showed submucosal invasion. These results indicate that the KK‐Ay mouse, featuring intact leptin and leptin receptor Ob‐Rbl, could be a useful animal model to investigate obesity‐associated cancer.
In vivo study of the effect of mechanical compression on formation of OCT images of human skin
Authors: Kirillin Mikhail Yu. , Agrba Pavel D. , Kamensky Vladislav A. 
Published: September 2010

AbstractModern optical diagnostic techniques often require deformations of the studied biotissues for image acquisition. This paper discusses the effect of mechanical compression on the formation of OCT images of human skin. The study was performed in vivo on human volunteers of different age. We show that application of compression to human skin induces changes in optical properties of the sample associated with elasticity of different skin layers. These changes induce an increase in the contrast of interlayer boundaries. Further application of compression causes the appearance of dark areas in the OCT images obtained, likely associated with interstitial or intracellular water inflow to the observed region. The effects studied are of importance for proper interpretation of obtained OCT images in diagnosis of skin pathologies. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Quantitative BOLD: The effect of diffusion
Authors: Dickson John D. , Ash Tom W.J. , Williams Guy B. , Harding Sally G. , Carpenter T. Adrian , Menon David K. , Ansorge Richard E. 
Published: September 2010

AbstractPurpose:To make the quantitative blood oxygenation level‐dependent (qBOLD) method more suitable for clinical application by accounting for proton diffusion and reducing acquisition times.Materials and Methods:Monte Carlo methods are used to simulate the signal from diffusing protons in the presence of a blood vessel network. A diffusive qBOLD model was then constructed using a lookup table of the results. Acquisition times are reduced by parallel imaging and by employing an integrated fieldmapping method, rather than running an additional sequence.Results:The addition of diffusion to the model is shown to have a significant impact on predicted signal formation. This is found to affect all fitted parameters when the model is applied to real data. Parallel imaging and integrated fieldmapping allowed the GESSE (gradient echo sampling of a spin echo) acquisition to be made in less than 10 minutes while maintaining high signal‐to‐noise ratio (SNR).Conclusion:By incorporating integrated field mapping and parallel imaging techniques, GESSE data were acquired within clinically acceptable acquisition times. These data fit closely to the diffusive qBOLD model, providing more realistic and robust measurements of T2 and blood oxygenation than the static model. J. Magn. Reson. Imaging 2010;32:953–961. © 2010 Wiley‐Liss, Inc.
Device for sectioning prostatectomy specimens to facilitate comparison between histology and in vivo MRI
Authors: Drew Bryn , Jones Edward C. , Reinsberg Stefan , Yung Andrew C. , Goldenberg S. Larry , Kozlowski Piotr 
Published: September 2010

AbstractPurposeTo develop a device for sectioning prostatectomy specimens that would facilitate comparison between histology and in vivo MRI.Materials and MethodsA multi‐bladed cutting device was developed, which consists of an adjustable box capable of accommodating a prostatectomy specimen up to 85 mm in size in the lateral direction, a “plunger” tool to press on the excised gland from the top to prevent it from rolling or sliding during sectioning, and a multi‐bladed knife assembly capable of holding up to 21 blades at 4‐mm intervals. The device was tested on a formalin fixed piece of meat and subsequently used to section a prostatectomy specimen. Histology sections were compared with T2‐weighted MR images acquired in vivo before the prostatectomy procedure.ResultsThe prostatectomy specimen slices were very uniform in thickness with each face parallel to the other with no visible sawing marks on the sections by the blades after the cut. MRI and histology comparison showed good correspondence between the two images.ConclusionThe developed device allows sectioning of prostatectomy specimens into parallel cuts at a specific orientation and fixed intervals. Such a device is useful in facilitating accurate correlation between histology and MRI data. J. Magn. Reson. Imaging 2010;32:992–996. © 2010 Wiley‐Liss, Inc.
Neuropathological differences between rats and mice after spinal cord injury
Authors: Byrnes Kimberly R. , Fricke Stanley T. , Faden Alan I. 
Published: September 2010

AbstractPurposeTo investigate the utility of noninvasive magnetic resonance imaging (MRI) protocols to demonstrate pathological differences between rats and mice after spinal cord injury (SCI). Rats and mice are commonly used to model SCI; however, histology and immunohistochemistry have shown differences in neuropathology between the two species, including cavity formation and scar/inflammatory responses.Materials and MethodsModerate contusion SCI was performed on adult male rats and mice. At 28 days postinjury, animals underwent T1‐weighted (T1W), with or without gadolinium contrast, or T2‐weighted (T2W) magnetic resonance imaging (MRI), to be compared with histology at the same timepoint.ResultsIn both species, all MRI methods demonstrated changes in spinal cord anatomy. Immunohistochemistry indicated that T2W accurately reflected areas of inflammation and glial scar formation in rats and mice. Quantitation of lesion volume by histology and functional performance correlated best with T2W measurements in both species. Gadolinium contrast accurately reflected the blood‐spinal cord‐barrier permeability in both species, which appeared greater in rats than in mice.ConclusionThese data demonstrate that MRI, with either a T1W or T2W protocol, can effectively distinguish pathological differences between rats and mice. J. Magn. Reson. Imaging 2010;32:836–846. © 2010 Wiley‐Liss, Inc.
Mechanical loading increased BMP‐2 expression which promoted osteogenic differentiation of tendon‐derived stem cells
Authors: Rui Yun Feng , Lui Pauline Po Yee , Ni Ming , Chan Lai Shan , Lee Yuk Wa , Chan Kai Ming 
Published: September 2010

AbstractThis study aimed to investigate the effect of repetitive tensile loading on the expression of BMP‐2 and the effect of BMP‐2 on the osteogenic differentiation of tendon‐derived stem cells (TDSCs) in vitro. Repetitive stretching was applied to TDSCs isolated from rat patellar tendon at 0%, 4%, and 8%, 0.5 Hz. The expression of BMP‐2 was detected by Western blotting and qPCR. To study the osteogenic effects of BMP‐2 on TDSCs, BMP‐2 was added to the TDSC monolayer for the detection of ALP activity and calcium nodule formation in a separate experiment. TDSCs adhered, proliferated, and aligned along the direction of externally applied tensile force while they were randomly oriented in the control group. Western blotting showed increased expression of BMP‐2 in 4% and 8% stretching groups but not in the control group. Up‐regulation of BMP‐2 mRNA was also observed in the 4% stretching group. BMP‐2 increased the osteogenic differentiation of TDSCs as indicated by higher ALP cytochemical staining, ALP activity, and calcium nodule formation. Repetitive tensile loading increased the expression of BMP‐2 and addition of BMP‐2 enhanced osteogenic differentiation of TDSCs. Activation of BMP‐2 expression in TDSCs during tendon overuse might provide a possible explanation of ectopic calcification in calcifying tendinopathy. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:390–396, 2011
Variations in varus/valgus and internal/external rotational knee laxity and stiffness across the menstrual cycle
Authors: Shultz Sandra J. , Schmitz Randy J. , Beynnon Bruce D. 
Published: September 2010

AbstractCyclic variations in genu recurvatum (GR), general joint laxity (GJL), varus–valgus (VV), and internal–external (IER) rotational laxities and stiffnesses were examined in 64 females and 43 males at two time points during the females' menstrual cycle [days of minimum (T1) and maximum (T2) anterior knee laxity (AKL)]. Cyclic increases in AKL (9.5%), GR (37.5%), and GJL (13.6%) were observed in females but not males from T1 to T2 (p < 0.001). Cyclic increases in VV and IER laxity were negligible (1.5–3.2%, p > 0.320). Females compared to males had lower overall VV stiffness at T2 (F 37% <M) vs. T1 (F 26.9% <M; p = 0.011), but no difference across time points for IER stiffness (p = 0.452). Across both time points, females had consistently greater VV (30.2%) and IER (20%) laxity and less VV (32.5%) and IER (24.3%) incremental stiffness (p < 0.001). Low‐to‐moderate associations were observed between AKL, GR, and GJL with VV and IER laxities and stiffnesses in females as measured at T1 and the change in values from T1 to T2. Whether these findings reflect ligament‐specific responses to hormone changes, or implicate changes in injury risk potential across the menstrual cycle requires further study. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:318–325, 2011
Tendon fascicle gliding in wild type, heterozygous, and lubricin knockout mice
Authors: Kohrs Ross T. , Zhao Chunfeng , Sun Yu‐Long , Jay Gregory D. , Zhang Ling , Warman Matthew L. , An Kai‐Nan , Amadio Peter C. 
Published: September 2010

AbstractThe objective of this study was to investigate the role of lubricin in the lubrication of tendon fascicles. Lubricin, a glycoprotein, lubricates cartilage and tendon surfaces, but the function of lubricin within the tendon fascicle is unclear. We developed a novel method to assess the gliding resistance of a single fascicle in a mouse tail model and used it to test the hypothesis that gliding resistance would be increased in lubricin knockout mice. Thirty‐six mouse tails were used from 12 wild type, 12 heterozygous, and 12 lubricin knockout mice. A 15 mm long fascicle segment was pulled proximally after being divided distally. The peak resistance during fascicle pullout and the fascicle perimeter were measured. Lubricin expression was evaluated by immunohistochemistry. The peak gliding resistance in the lubricin knockout mice was significantly higher than in the wild type (p < 0.05). Fascicles from heterozygous mice were intermediate in value, but not significantly different from either wild type or lubricin knockout fascicles in peak gliding resistance. No significant difference was found in fascicle perimeter among the three groups. No correlation was observed between fascicle perimeter and gliding resistance. While lubricin was detected by immunostaining on the fascicle surface in wild type and heterozygous mice, lubricin was not detectable in the tendons of knockout mice. We conclude that the absence of lubricin is associated with increased interfascicular friction and that lubricin may play an important role in interfascicular lubrication. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:384–389, 2011
The role of omental flap transposition in patients with locoregional recurrent rectal cancer treated with reirradiation
Authors: Kim Tae Hyun , Kim Dae Yong , Jung Kyung Hae , Hong Yong Sang , Kim Sun Young , Park Ji Won , Lim Seok‐Byung , Choi Hyo Seong , Jeong Seung‐Yong , Oh Jae Hwan 
Published: September 2010

AbstractBackground and ObjectivesWe performed reirradiation after omental flap transposition (OFT) in patients with locoregional recurrent rectal cancer (LRRC) and evaluated the effect of OFT on the irradiated small bowel by comparing the displacement of the small bowel from the radiation field before and after OFT.MethodsBetween October 2005 and October 2008, this study included 12 patients with LRRC who had previously received radiotherapy. To evaluate the effect of OFT on the irradiated volume of the small bowel and bladder, we measured the closest distances between the small bowel and the tumor or tumor bed (distanceSB) and between the bladder and tumor or tumor bed (distanceBL) before and after OFT, respectively.ResultsThe median distanceSB before and after OFT was 5 and 30 mm, respectively (P < 0.001). The median distanceBL before and after OFT was 10 and 23 mm, respectively (P = 0.002). The respective overall survival and local control rates at 3 years were 50.9% and 54.6%, respectively. No severe complication occurred of grade 3 or higher involving the small bowel or bladder.ConclusionsIn our study, OFT effectively excluded small bowel from the radiation field. In addition, selective reirradiation after OFT was feasible for patients with LRRC. J. Surg. Oncol. 2010;102:789–795. © 2010 Wiley‐Liss, Inc.
Evaluation of environmental contamination by platinum and exposure risks for healthcare workers during a heated intraperitoneal perioperative chemotherapy (HIPEC) procedure
Authors: Konate Armande , Poupon Joël , Villa Antoine , Garnier Robert , Hasni‐Pichard Hélène , Mezzaroba Danielle , Fernandez Gabriel , Pocard Marc 
Published: September 2010

AbstractThe study was plan to assess platinum (Pt) contamination in the operating room and its exposure to health workers during heated intraperitoneal perioperative chemotherapy (HIPEC) using oxaliplatin.Materials and MethodsPt was measured in urinary and environmental (air and surfaces) samples via inductively coupled plasma mass spectrometry (ICP‐MS). Urinary samples were obtained from 11 members of the staff before and after the procedure and from 6 controls. Samples from 15 surfaces and from 3 filters from the air extractors were also analyzed for Pt contamination.ResultsBefore HIPEC, Pt levels in urinary samples were similar in both the exposed and control groups; concentrations were below the limit of detection (i.e., 1.5 ng/L). No elevation was observed in the exposed group at the end of the procedure. Surgeon gloves were heavily contaminated. On other analyzed surfaces, lesser amounts of Pt were measured, ranging from 2 ng on the surgeon's hands to 183 ng on the forceps. All three air filters tested negative.ConclusionNo contamination of healthcare workers or of the air in the operating room was detected. However, the heavy contamination of the surgeon's gloves demonstrates why doubling of specialized gloves for the surgeon should be mandatory. J. Surg. Oncol. 2011;103:6–9. © 2010 Wiley‐Liss, Inc.
Interferon‐α therapy after curative resection prevents early recurrence and improves survival in patients with hepatitis B virus‐related hepatocellular carcinoma
Authors: Qu Li‐Shuai , Jin Fei , Huang Xiao‐Wu , Shen Xi‐Zhong 
Published: September 2010

AbstractBackgroundA retrospective cohort study was conducted to investigate the effect of interferon‐α (IFN‐α) therapy after curative resection on survival and recurrence in patients with hepatitis B virus (HBV)‐related hepatocellular carcinoma (HCC).MethodsOf 568 HBV‐related HCC patients who underwent curative resection, 101 patients received postoperative IFN‐α therapy (5 million units three times every week for 18 months). Clinicopathological factors were compared between patients with postoperative IFN‐α therapy or not. Risk factors for survival, early, and late recurrence (2 years as cut‐off) were studied.ResultsThe median follow‐up time was 53.3 months. There was no significant difference in clinicopathological factors between the two groups. Patients with postoperative IFN‐α therapy had higher overall survival rates (hazards ratio (HR): 0.612, 95% confidence interval (CI): 0.422–0.889, P = 0.010). No significant difference in disease‐free survival rates was detected between the two groups (HR: 0.786, 95% CI: 0.597–1.035, P = 0.086). Multivariate analysis revealed that postoperative IFN‐α therapy was an independent factor for overall survival (HR: 0.611, 95% CI: 0.421–0.887, P = 0.010) and significantly reduced early recurrence (HR: 0.562, 95% CI: 0.375–0.840, P = 0.005).ConclusionsIFN‐α therapy after curative resection prevented early recurrence and improved overall survival of patients with HBV‐related HCC. J. Surg. Oncol. 2010;102:796–801. © 2010 Wiley‐Liss, Inc.
The association of frequent allelic loss on 17p13.1 with early metastastic recurrence of hepatocellular carcinoma after liver transplantation
Authors: Zhou Lin , Zhou Wuhua , Wu Liming , Yu Xiaobo , Xing Chunyang , Zheng Shusen 
Published: September 2010

AbstractBackground and ObjectivesIdentification and characterization of loss of heterozygosity (LOH) can determine putative tumor suppressor genes (TSGs) and provide a variety of molecular markers for hepatocellular carcinoma (HCC). This study aimed to investigate LOH status on chromosomes 4q, 6q, 8p, 9p, and 17p, and to explore their clinical significances in HCC post‐liver transplantation.MethodsA total of 37 patients with HCC who underwent liver transplantation were enrolled. LOH was examined using 34 microsatellite markers located on 4q13‐3q5, 6q27, 8p22‐p23, 9p21‐p22, and 17p12‐p13.ResultsThe frequency of LOH at each microsatellite locus ranged from 23% to 75%, with a mean value of 53.1%. Frequencies of LOH on 4q, 6q, 8p, 9p, and 17p were 62% (23 of 37), 30% (11 of 37), 49% (18 of 37), 46% (16 of 35), and 68% (25 of 37), respectively. LOHs on certain chromosomal regions were significantly associated with age, AFP level, tumor size, tumor multiplicity, histological grade, and metastatic recurrence.ConclusionsLOH on 17p13.1 correlated to metastatic HCC recurrence, while LOH on 4q and 8p was found to be associated with progression of HCC. Thus, potential novel biomarkers or TSGs for prognosis and treatment of HCC may harbor on these regions. J. Surg. Oncol. 2010;102:802–808. © 2010 Wiley‐Liss, Inc.
Differential effect of hyperthermia on nerves and smooth muscle of the mouse ileum
Authors: Burke Shoshana , Abu‐Wasel Basam , Eid Ahmed , Nissan Aviram , Hanani Menachem 
Published: September 2010

AbstractBackground and ObjectivesCytoreductive surgery (CRS) and hyperthermic intraperitoneal chemotherapy (HIPEC) gained wide acceptance as the treatment of choice for selected patients with peritoneal surface malignancies. Patients tend to suffer from prolonged ileus following CRS + HIPEC, complicating their recovery. We studied the effects of hyperthermia on the intestine to gain insight into mechanisms of ileus post‐HIPEC.MethodsSegments of mouse ileum were incubated at 36°C. Electrical field stimulation (EFS) was applied, stimulating nerves, and the resultant muscle contraction was measured. The response was measured at varying temperatures (38–43°C) at exposure times of up to 120 min. We also stimulated the tissues with 10−6 M carbachol, a muscarinic receptor agonist, which acts directly on smooth muscle.ResultsResponse to EFS decreased at high temperatures, especially above 41°C. This effect was irreversible for 120 min after decreasing temperature. When stimulating with carbachol, both transient and plateau responses decreased at 43°C (plateau more than transient) but the effect reversed on returning to 36°C.ConclusionThe irreversible decline in responses to nerve stimulation when exposed to high temperatures was not seen with direct muscle stimulation. This indicates that smooth muscle is resilient and that the main effect of hyperthermia is on nerves. These results have significance for HIPEC. J. Surg. Oncol. 2011;103:92–100. © 2010 Wiley‐Liss, Inc.
Rapid separation and immunoassay for low levels of Salmonella in foods using magnetosome–antibody complex and real‐time fluorescence quantitative PCR
Authors: Li Aihua , Zhang Huiyuan , Zhang Xin , Wang Qi , Tian Jiesheng , Li Ying , Li Jilun 
Published: September 2010

AbstractA rapid and economical method for detecting Salmonella was developed, based on a novel complex for immunomagnetic separation, which was composed of anti‐Salmonella polyclonal antibody (Ab) and magnetosome (bacterial magnetic particle, BMP) produced by the bacterium Magnetospirillum gryphiswaldense MSR‐1. BMP‐Ab complex was used to capture Salmonella from pure suspensions of S. dublin, S. enteritidis, S. aesch, S. agona, S. abony and S. bareily, from mixed suspensions of S. dublin and Vibrio parahaemolyticus, and from artificially contaminated food samples. Captured Salmonella were then detected by plate count, or real‐time fluorescence quantitative PCR. Capture efficiencies, calculated from plate count, were >80% for the pure Salmonella suspensions of all six strains, and >70% for the mixed suspension. Samples of six food products, with artificial contamination by 6000, 600, 60, or 0.6 cfu/mL S. dublin, were captured by complex and detected by real‐time fluorescence quantitative PCR. Threshold cycle values varied depending on type of food. The lower limit of detectability was 60 cfu/mL without pre‐enrichment, and <0.6 cfu/mL after 3‐h pre‐enrichment. The method described here, based on capture pathogens by BMP‐Ab complex, is sensitive, rapid, and considerably simpler than traditional methods for Salmonella detection. It can be extended to other pathogens by the use of appropriate antibodies.