bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plexin domain-containing 1 may be a biomarker of poor prognosis in hepatocellular carcinoma patients, may mediate immune evasion
Authors: Tang Ming-Yue , Shen Xue , Yuan Run-Sheng , Li Hui-Yuan , Li Xin-Wei , Jing Yi-Ming , Zhang Yue , Shen Hong-Hong , Wang Zi-Shu , Zhou Lei , Yang Yun-Chuan , Wen He-Xin , Su Fang 
Published: May 2024

BACKGROUND
For the first time, we investigated the oncological role of plexin domain-containing 1 (PLXDC1 ), also known as tumor endothelial marker 7 (TEM7), in hepatocellular carcinoma (HCC).
AIM
To investigate the oncological profile of PLXDC1 in HCC.
METHODS
Based on The Cancer Genome Atlas database, we analyzed the expression of PLXDC1 in HCC. Using immunohistochemistry, quantitative real-time polymerase chain reaction (qRT-PCR), and Western blotting, we validated our results. The prognostic value of PLXDC1 in HCC was analyzed by assessing its correlation with clinicopathological features, such as patient survival, methylation level, tumor immune microenvironment features, and immune cell surface checkpoint expression. Finally, to assess the immune evasion potential of PLXDC1 in HCC, we used the tumor immune dysfunction and exclusion (TIDE) website and immunohistochemical staining assays.
RESULTS
Based on immunohistochemistry, qRT-PCR, and Western blot assays, overexpression of PLXDC1 in HCC was associated with poor prognosis. Univariate and multivariate Cox analyses indicated that PLXDC1 might be an independent prognostic factor. In HCC patients with high methylation levels, the prognosis was worse than in patients with low methylation levels. Pathway enrichment analysis of HCC tissues indicated that genes upregulated in the high-PLXDC1 subgroup were enriched in mesenchymal and immune activation signaling, and TIDE assessment showed that the risk of immune evasion was significantly higher in the high-PLXDC1 subgroup compared to the low-PLXDC1 subgroup. The high-risk group had a significantly lower immune evasion rate as well as a poor prognosis, and PLXDC1 -related risk scores were also associated with a poor prognosis.
CONCLUSION
As a result of this study analyzing PLXDC1 from multiple biological perspectives, it was revealed that it is a biomarker of poor prognosis for HCC patients, and that it plays a role in determining immune evasion status.
Immunomodulation of adipose-derived mesenchymal stem cells on peripheral blood mononuclear cells in colorectal cancer patients with COVID-19
Authors: Wang Jun-Feng , Yang Xiao-Xia , Zhang Jian , Zheng Yan , Zhang Fu-Qing , Shi Xiao-Feng , Wang Yu-Liang 
Published: May 2024

BACKGROUND
Accumulating evidence has shown that adipose tissue-derived mesenchymal stem cells (ADSCs) are an effective therapeutic approach for managing coronavirus disease 2019 (COVID-19); however, further elucidation is required to determine their underlying immunomodulatory effect on the mRNA expression of T helper cell-related transcription factors (TFs) and cytokine release in peripheral blood mononuclear cells (PBMCs).
AIM
To investigate the impact of ADSCs on the mRNA expression of TFs and cytokine release in PBMCs from colorectal cancer (CRC) patients with severe COVID-19 (CRC+ patients).
METHODS
PBMCs from CRC+ patients (PBMCs-C+ ) and age-matched CRC patients (PBMCs-C) were stimulated and cultured in the presence/absence of ADSCs. The mRNA levels of T-box TF TBX21 (T-bet), GATA binding protein 3 (GATA-3), RAR-related orphan receptor C (RORC), and forkhead box P3 (FoxP3) in the PBMCs were determined by reverse transcriptase-polymerase chain reaction. Culture supernatants were evaluated for levels of interferon gamma (IFN-γ), interleukin 4 (IL-4), IL-17A, and transforming growth factor beta 1 (TGF-β1) using an enzyme-linked immunosorbent assay.
RESULTS
Compared with PBMCs-C, PBMCs-C+ exhibited higher mRNA levels of T-bet and RORC, and increased levels of IFN-γ and IL-17A. Additionally, a significant decrease in FoxP3 mRNA and TGF-β1, as well as an increase in T-bet/GATA-3, RORC/FoxP3, IFN-γ/IL-4, and IL-17A/TGF-β1 ratios were observed in PBMCs-C+ . Furthermore, ADSCs significantly induced a functional regulatory T cell (Treg) subset, as evidenced by an increase in FoxP3 mRNA and TGF-β1 release levels. This was accompanied by a significant decrease in the mRNA levels of T-bet and RORC, release of IFN-γ and IL-17A, and T-bet/GATA-3, RORC/FoxP3, IFN-γ/IL-4, and IL-17A/TGF-β1 ratios, compared with the PBMCs-C+ alone.
CONCLUSION
The present in vitro studies showed that ADSCs contributed to the immunosuppressive effects on PBMCs-C+ , favoring Treg responses. Thus, ADSC-based cell therapy could be a beneficial approach for patients with severe COVID-19 who fail to respond to conventional therapies.
Loss of heterozygosity for chromosomes 16q in Wilms tumors predicts outcomes: A meta-analysis
Authors: Song Yuan-Hua , Li Wen-Ling , Yang Zhen , Gao Yan , Feng Zhi-Ping 
Published: May 2024

BACKGROUND
The research findings suggest that the prognosis of children with Wilms tumor (WT) is affected by various factors. Some scholars have indicated that loss of heterozygosity (LOH) on chromosome 16q is associated with a poor prognosis in patients with WT.
AIM
To further elucidate this relationship, we conducted a meta-analysis.
METHODS
This meta-analysis was registered in INPLASY (INPLASY2023100060). We systematically searched databases including Embase, PubMed, Web of Science, Cochrane, and Google Scholar up to May 31, 2020, for randomized trials reporting any intrapartum fetal surveillance approach. The meta-analysis was performed within a frequentist framework, and the quality and network inconsistency of trials were assessed. Odds ratios and 95%CIs were calculated to report the relationship between event-free survival and 16q LOH in patients with WT.
RESULTS
Eleven cohort studies were included in this meta-analysis to estimate the relationship between event-free survival and 16q LOH in patients with WT (I 2 = 25%, P < 0.001). As expected, 16q LOH can serve as an effective predictor of event-free survival in patients with WT (risk ratio = 1.95, 95%CI: 1.52–2.49, P < 0.001).
CONCLUSION
In pediatric patients with WT, there exists a partial correlation between 16q LOH and an unfavorable treatment prognosis. Clinical detection of 16q chromosome LOH warrants increased attention to the patient’s prognosis.
Association of complement components with risk of colorectal cancer: A systematic review and meta-analysis
Authors: Zhu Xiao-Lin , Zhang Lu , Qi Su-Xia 
Published: May 2024

BACKGROUND
Complement components could contribute to the tumor microenvironment and the systemic immune response. Nevertheless, their role in colorectal cancer (CRC) remains a contentious subject.
AIM
To elucidate the relationship between complement components and CRC risk and clinical characteristics.
METHODS
Searches were conducted in PubMed, the Cochrane Library, and the China National Knowledge Infrastructure database until June 1, 2023. We included cohort studies encompassing participants aged ≥ 18 years, investigating the association between complement components and CRC. The studies were of moderate quality or above, as determined by the Agency for Healthcare Research and Quality. The meta-analysis employed fixed-effects or random-effects models based on the I² test, utilizing risk ratio (RR) and their corresponding 95% confidence interval (CI) for outcomes. Sensitivity and subgroup analyses were performed to validate the robustness of the collective estimates and identify the source of heterogeneity.
RESULTS
Data from 15 studies, comprising 1631 participants that met the inclusion criteria, were included in the meta-analysis. Our findings indicated that protein levels of cluster of differentiation 46 (CD46) (RR = 3.66, 95%CI: 1.75-7.64, P < 0.001), CD59 (RR = 2.86, 95%CI: 1.36-6.01, P = 0.005), and component 1 (C1) (RR = 5.88, 95%CI: 1.75-19.73, P = 0.004) and serum levels of C3 (standardized mean difference = 1.82, 95%CI: 0.06-3.58, P = 0.040) were significantly elevated in patients with CRC compared to healthy controls. Strong expression of CD55 or CD59 was associated with a higher incidence of lymph node metastasis, whereas strong CD46 expression correlated with a higher incidence of tumor differentiation compared to low CD46 expression (P < 0.05 for all). Although specific pooled results demonstrated notable heterogeneity, subgroup analyses pointed to regional differences as the primary source of inconsistency among the studies.
CONCLUSION
Our analysis underscores that increased levels of specific complement components are associated with a heightened risk of CRC, emphasizing the potential significance of monitoring elevated complement component levels.
Multidisciplinary comprehensive treatment of massive hepatocellular carcinoma with hemorrhage: A case report and review of literature
Authors: Kou Xian-Shuai , Li Fan-Fan , Meng Yun , Zhao Jian-Ming , Liu Sheng-Fen , Zhang Lan 
Published: May 2024

BACKGROUND
Hepatocellular carcinoma (HCC), a major contributor to cancer-related deaths, is particularly prevalent in Asia, largely due to hepatitis B virus infection. Its prognosis is generally poor. This case report contributes to the medical literature by detailing a unique approach in treating a large HCC through multidisciplinary collaboration, particularly in patients with massive HCC complicated by ruptured bleeding, a scenario not extensively documented previously.
CASE SUMMARY
The patient presented with large HCC complicated by intratumoral bleeding. Treatment involved a multidisciplinary approach, providing individualized care. The strategy included drug-eluting bead transarterial chemoembolization, sorafenib-targeted therapy, laparoscopic partial hepatectomy, and standardized sintilimab monoclonal antibody therapy. Six months after treatment, the patient achieved complete radiological remission, with significant symptom relief. Imaging studies showed no lesions or recurrence, and clinical assessments confirmed complete remission. This report is notable as possibly the first documented case of successfully treating such complex HCC conditions through integrated multidisciplinary efforts, offering new insights and a reference for future similar cases.
CONCLUSION
This study demonstrated effective multidisciplinary treatment for massive HCC with intratumoral bleeding, providing insights for future similar cases.
Mills, Florence (1895 or 1896–1927)
Authors: Cubbon Alexandra 
Published: May 2024

Florence Mills was a leading African American performer of the Harlem Renaissance. Born in Washington, D.C. in the mid-1890s, Mills was raised in Harlem, New York City, and began performing as a child. During her career as a singer, dancer, and comedian, she performed vaudeville, burlesque, and eventually crossed over into mainstream Broadway, garnering international fame along the way. Mills fought for racial equality throughout her lifetime, using her voice both on and off stage to speak out against racism.
Among the productions Mills starred in, some of the most notable and historic were Folly Town (1920), Shuffle Along (1921), and The Black Birds Revue (1926). Folly Town was among the first racially integrated shows in New York, while Shuffle Along proved that musicals produced by African Americans could appeal to interracial audiences. Mills was also a celebrated singer, whose song ‘I’m a Little Blackbird Looking for a Bluebird’ called for racial justice. Although she died an untimely death in 1927 at the age of 31, Mills’ years of work on the stage challenged contemporary norms around race, class, and gender and contributed to the modernization of theatrical performance.
Dark fondas, una opción para mejorar la economía de las mujeres
Authors: Rivera Rojas Carlos de Jesús 
Published: May 2024

“En cinco años me veo con mi propio negocio, mis propias ganancias para que yo pueda decidir y hacer más cosas”, fue lo que comentó Noemi, una mujer desempleada de 56 años durante nuestra entrevista (N. Casillas, comunicación personal, noviembre de 2022), quien al mencionar su plan a corto plazo demuestra una clara consciencia de su situación actual y determinación por cambiarla para tomar el control de su futuro.
En los siguientes apartados explicaré las adversidades y circunstancias sociales que han llevado a Noemí y a miles de mujeres más en México a enfrentar una combinación de desempleo, pobreza laboral, trabajo no remunerado y vulnerabilidad social entre otras fricciones. Finalmente, se plantea un modelo que espera responder a esta problemática a través de la preparación de comida casera como una opción para recuperar su autonomía financiera: el de las dark fondas.
Sientocolor: Diseño de un sistema táctil que le permita a las personas con discapacidad visual identificar los colores de las prendas de ropa de manera independiente
Authors: Tanaka Tsutsumi Harumi Sofía , Díaz Villaseñor Alfonso , Magaña Fajardo Carolina 
Published: May 2024

La sociedad ha estandarizado entornos y actividades, lo que causa problemas a quienes no encajan en el promedio, dando paso a la discapacidad. El objetivo de esta investigación es mejorar la experiencia de compra en tiendas departamentales en la Ciudad de México en 2022 para personas con discapacidad (PcD). Se emplea una metodología centrada en las PcD, usando herramientas como el marco AEIOU, LEGO® Serious Play® y dinámicas de co-diseño para fomentar la colaboración y reflexionar sobre el papel de los diseñadores. El objetivo es desarrollar soluciones que faciliten la elección de colores por parte de las PcD, promoviendo un proceso de desarrollo accesible e inclusivo. De esta manera se aumentará la independencia y autonomía de este grupo de la población y reducir las incompatibilidades que surgen de la estandarización social en entornos y actividades.

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Diseño de servicios para personas mayores mexicanas: una experiencia de codiseño en el aula
Authors: García y Colomé G. Ana Paula , Maya Rivero Annika 
Published: May 2024

El incremento de la esperanza de vida obliga a repensar cómo proyectamos el mundo que nos rodea, por ello, en la clase de Diseño de Servicios, impartida en el Centro de Investigaciones de Diseño Industrial de la UNAM, se llevó a cabo el proyecto “Codiseño de servicios para personas mayores”, donde participaron nueve mujeres mayores de sesenta años y diecinueve estudiantes de la Licenciatura en Diseño Industrial. El objetivo fue codiseñar servicios para personas mayores partiendo de la definición de oportunidades, bajo la metodología de Diseño de Servicios. Los resultados consistieron en cinco propuestas: plataforma de aprendizaje y socialización, aplicación para planificar y gestionar viajes, programa de gobierno para promoción y venta de productos artesanales y manualidades, gimnasio para mayores, y un servicio de comida saludable a domicilio.
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Upskate: construcción de comunidad a través del skate y el diseño
Authors: Norzagaray Natera Andrea 
Published: May 2024

Este escrito documenta y comparte los hallazgos encontrados en el codiseño e implementación de Upskate: taller del upcycling de tablas de skate que tuvo como objetivo fomentar los vínculos entre las personas que comparten el interés por el skate en el área de influencia de CENTRO. Los participantes transformaron sus tablas rotas o astilladas en muebles reaprovechando de esta manera los desechos de esta práctica. Los hallazgos del estudio sugieren que la colaboración entre diseño difuso y experto puede ser una forma eficaz para fomentar la construcción de comunidad. El escrito explora la historia del skate y su relación con la creatividad, el desarrollo del skatepark de Constituyentes y la misión del Hub de diseño social de CENTRO. Posteriormente, describe la metodología, las etapas del proyecto y sus resultados e impacto en ambas comunidades.
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Effects of Safe and Sound Protocol on self-reported autonomic reactivity, anxiety, and depression in speech therapy clients with voice, throat, and breathing complaints
Authors: Grooten-Bresser Heleen , Heilman Keri J. , Kooijman Piet G. C. , Monti Elisa , Chenault Mickey N. , Holmes Logan G. , Resto Olivia 
Published: May 2024

Medically unexplained oto-rhino-laryngeal symptoms (MUORLS) may have a pathophysiology related to brain-body state regulation. We use predictions from the Polyvagal Theory to investigate whether clients with voice problems have increased autonomic reactivity, anxiety, and depression. Study 1 included 54 clients with MUORLS, and a comparison group of 26 individuals without MUORLS. Outcome measures were anxiety and depression (Hospital Anxiety and Depression Scale, HADS), autonomic reactivity (Body Perception Questionnaire Short Form, BPQ-SF), and extent of voice handicap (The Voice Handicap Index, VHI) for the clients. The client group scored higher than the control group in supra-diaphragmatic reactivity, but there were no group differences in other measures. To extend the findings of Study 1, a feasibility study (Study 2) evaluated the effects of the Safe and Sound Protocol (SSP), a Polyvagal-informed protocol, on the same outcome measures. Study 2 included 33 clients with self-reported voice and throat complaints. Outcome measures assessed pre- and post-SSP were anxiety and depression (HADS) and autonomic reactivity (BPQ-SF). Results demonstrated a significant decrease in anxiety, depression, and autonomic reactivity post-SSP. In conclusion, a better understanding of the mechanisms that underlie MUORLS may provide guidance for using neurophysiologically based interventions, such as SSP, that target autonomic state regulation.
The Impact of Early Pulmonary Rehabilitation on Dyspnea, Quality of Life, and Symptoms in Moderate to Severe COVID-19 Pneumonia Patients
Authors: Phimphasak Chatchai , Khotsopa Tulyachat , Nwe Aung Aung , Chumpangern Worawat , Reechaipichitkul Wipa , Ubolsakka-Jones Chulee , So-Ngern Apichart , Ratanawatkul Pailin , Arunsurat Ittipat , Kosura Nimit , Audsavachulamanee Buranat 
Published: May 2024

Introduction: COVID-19 pneumonia leads to dyspnea, persistent symptoms and poor quality of life. Inpatient pulmonary rehabilitation (PR) has limited evidence of effectiveness. Therefore, we aimed to determine the effect of a comprehensive inpatient PR program on dyspnea symptoms and quality of life in patients with COVID-19 pneumonia. Methods: A group pretest-posttest design was conducted in patients with COVID-19 pneumonia. Comprehensive PR was administered during hospitalization. Baseline dyspnea index (BDI), Transitional dyspnea score (TDI), Modified Medical Research Council score (mMRC), and St. George’s Respiratory Questionnaire (SGRQ) were assessed at baseline, 4th week and 12th week after discharge. Results: In total, 28 patients were included (9 with oxygen supplementation, 19 without oxygen supplementation). The mean BDI at baseline was 7.82 ± 2.13. TDI scores at the 4th and 12th weeks were 5.14 ± 1.90 and 6.61 ± 1.59, respectively, with a significant difference observed between the 4th and 12th weeks (p < 0.001). Median mMRC scores at baseline, 4th week, and 12th week were 2(1.25 - 3), 1 (1 - 2) and 1 (0 - 1), respectively, showing a significant improvement between baseline vs 4th week, baseline vs 12th week, and 4th week vs 12th week (p < 0.001). The total SGRQ score exhibited a median (IQR) of 11.34 (7.88 - 8.58) at the 4th week and 6.93 (4.43 - 13.03) at the 12th week, demonstrating a statistically significant improvement (p < 0.001). No adverse events were reported during inpatient PR. Conclusion: Inpatient PR provides a safe and feasible profile, reduces dyspnea symptoms, and improves the quality of life in patients with moderate to severe COVID-19 pneumonia.
HIGHLIGHTS

COVID-19 pneumonia results in dyspnea and persistent symptoms, impacting quality of life.
Early pulmonary rehabilitation, from inpatient to a 12-week outpatient program for moderate and severe cases, is safe and improves dyspnea, quality of life, and patient reported symptoms.

GRAPHICAL ABSTRACT
Membentuk Akhlak Anak dengan Menghafal dan Tadabbur Al-Quran di Madrasah Alam Ya Bunayya Muara Bungo
Authors: Awaluddin  , Solihin Muhammad , Noviriani  
Published: May 2024

Al-Qur’an sebagai kitab suci pedoman hidup yang dijaga keasliannya oleh Allah SWT dengan banyak cara, salah satunya adalah melalui para penghafal al-Qur’an yang dipilih untuk menjaga kalam Allah yang Mulia di dalam fikiran dan hatinya. Oleh karena itu, banyak Madrasah yang berbasis Islam mengadakan program hafalan al-Quran sebagai salah satu program unggulan. Tujuan penelitian ini adalah untuk mengetahui penerapan metode al Jawarih di Madrasah Alam Ya Bunayya Bungo dan mengetahui implikasi metode al Jawarih dalam menghafal dan mempelajari al Qur’an terhadap akhlak santri di Madrasah Alam Ya Bunayya Bungo. Metode penelitan ini adalah deskriptif kualitatif.Penelitian ini di RA Alam Yaa Bunayya. Teknik pengumpulan data diambil dari observasi, wawancara dan dokumentasi. Hasil penelitian ini adalah penerapan menghafal ayat-ayat al Qur’an dengan menggunakan gerakan, tadabbur makna ayat perkata dan keseluruhan, dan implementasi memahami kandungan ayat dengan cara visual secara langsung membuat pembelajaran tahfidz sangatlah menyenangkan, tidak membosankan, dan sangat berkesan bagi para peserta didik di Madrasah Alam Ya Bunayya Bungo. Dan dari proses pembelajaran tersebut peserta didik merasakan kehadiran Allah Tuhan yang Maha Pengasih dan Maha penyayang selalu memberikan kebaikan-kebaikan kepada manusia sehingga muncullah rasa berkasih sayang sesama manusia serta syukur yang di aplikasikan dengan mengerjakan sholat, mengaji, belajar yang baik, berbuat kebajikan kepada orangtua guru dan kawan-kawan di Madrasah maupun di rumah.
PENERAPAN SANKSI TA’ZIR UNTUK MEMBENTUK KARAKTER DISIPLIN SANTRI DI PONDOK PESANTREN AL-MUSYARROFAH JAKARTA SELATAN
Authors: Oktavera Siska , Alfarisi Rifki , Ghofur Abdul 
Published: May 2024

Penelitian ini bertujuan mengetahui efek dari penerapan sanksi Ta’zir pada tingkat kedisiplinan santri di Pondok Pesantren al-Musyarrofah. Sering dijumpai di kalangan santri di banyak pondok pesantren yang melanggar peraturan-peraturan yang telah ditetapkan pengurus. Di antara hal yang menjadikan hal tersebut terjadi adalah kurangnya penanaman sikap disiplin pada santri dan tidak adanya konsekuensi terhadap peraturan-peraturan yang telah dilanggar. Penelitian ini menggunakan pendekatan kualitatif. Peneliti mendapatkan data menggunakan metode wawancara, observasi, dan dokumentasi. Teknik analisis data yang digunakan adalah reduksi data, penyajian data, dan penarikan kesimpulan. Hasil penelitian menyimpulkan bahwa penerapan ta’zir di Pondok Pesantren al-Musyarrofah disesuaikan dengan tingkatan pelanggaran yang telah dilakukan santri. Ta’zir yang diterapkan bentuknya bermacam-macam dan dikelompokkan menjadi 3 kategori, yaitu: ringan, sedang, dan berat. Penerapan ta’zir tersebut memberikan efek baik berupa meningkatnya kedisiplinan santri. Santri menjadi lebih mentaati peraturan yang ada dan melaksanakan kegiatan pesantren dengan baik. Kelebihan dan kekurangan yaitu tidak stabilnya semangat pengurus, kedua perlakuan yang kurang adil antara santri junior dan senior dalam menerapkan ta’zir, serta kurangnya tauladan dari santri senior.