bluuebunny/crossref_metadata_2025_split huggingface dataset:
N‐(2‐methyl‐6‐benzoxazolyl)‐N′‐1,5‐naphthyridin‐4‐yl urea (SB334867), a hypocretin receptor‐1 antagonist, preferentially prevents ethanol seeking: comparison with natural reward seeking
Authors: Martin‐Fardon Rémi , Weiss Friedbert 
Published: July 2012

AbstractOrexins/hypocretins (Orx/Hcrt) are hypothalamic peptides that regulate a wide range of physiological processes and have been shown to be recruited by drugs of abuse. This study was designed to test the effect of the specific Orx/Hcrt receptor‐1 (Hcrt‐r1) antagonist, N‐(2‐methyl‐6‐benzoxazolyl)‐N′‐1,5‐naphthyridin‐4‐yl urea (SB334867), on reinstatement elicited by ethanol (EtOH)‐associated stimuli versus stimuli associated with a conventional reinforcer [i.e. SuperSac, consisting of 3% glucose and 0.125% saccharin (w/v)]. SB334867 (1–10 mg/kg, i.p.) dose‐dependently reduced reinstatement induced by the EtOH‐ but not SuperSac‐associated stimuli. These findings support a differential role of Hcrt‐r1 in mediating EtOH seeking versus natural reward seeking.
Measurement of Ara h 1‐, 2‐, and 3‐specific IgE antibodies is useful in diagnosis of peanut allergy in Japanese children
Authors: Ebisawa Motohiro , Movérare Robert , Sato Sakura , Maruyama Nobuyuki , Borres Magnus P. , Komata Takatsugu 
Published: July 2012

To cite this article:Ebisawa M, Movérare R, Sato S, Maruyama N, Borres MP, Komata T. Measurement of Ara h 1‐, 2‐, and 3‐specific IgE antibodies is useful in diagnosis of peanut allergy in Japanese children.Pediatr Allergy Immunol2012:23: 573–581.AbstractBackground: Food challenges are time‐consuming, expensive, and not always possible to perform. Therefore, new tools to diagnose food allergy are desired. The aim was to evaluate IgE antibodies to peanut allergens in the diagnosis of peanut allergy in Japanese children using ImmunoCAP®and IgE immunoblotting.Methods: The study included 2–13‐yr‐old consecutive patients (n = 57) referred to our specialist clinic for investigation of current peanut allergy using food challenge. All children had a previous doctor’s diagnosis of peanut allergy and were on elimination diet. Serum samples were analyzed for IgE reactivity to peanut, recombinant (r) Ara h 1, 2, 3, 5, 8, and 9. IgE immunoblotting (n = 23) was performed using extracts from raw and roasted peanut.Results: Twenty‐six of the children failed (allergic group), and 31 passed the peanut challenge (tolerant group). The rAra h 2 ImmunoCAP test was superior in its ability to differentiate between children in the allergic and tolerant groups with a sensitivity and specificity of 88% and 84%, respectively (cutoff, 0.35 kUA/l). The combination of rAra h 1, 2, and 3 resulted in a higher specificity (94%) when IgE to all of them was the criteria for positivity. ImmunoCAP generally showed a good agreement with immunoblotting using both raw and roasted peanut for IgE reactivity to Ara h 1, 2, and 3.Conclusions: Measurement of IgE antibodies to rAra h 1, 2, and 3 is useful in the diagnosis of peanut allergy and in the investigation of reactions to raw and roasted peanut.
A major quantitative trait allele for resistance to the Sugarcane yellow leaf virus (Luteoviridae)
Authors: Costet Laurent , Raboin Louis‐Marie , Payet Magali , D’Hont Angélique , Nibouche Samuel 
Published: July 2012

With 1 figure and 1 tableAbstractThe yellow leaf disease of sugarcane is distributed worldwide and causes important yield losses. This disease is caused by a member of the Luteoviridae family, the Sugarcane yellow leaf virus (SCYLV). The objective of this study was to characterize genetic factors for resistance to yellow leaf disease in a sugarcane clone resistant to infection by SCYLV. We analysed a progeny of 196 clones obtained from a cross between the susceptible cultivar ‘R570’ and the resistant cultivar ‘MQ76‐53’. This progeny was assessed for virus incidence using tissue‐blot immunoassay after 10 years of cultivation under natural infection conditions. The progeny was genotyped with various types of molecular markers resulting in 2822 polymorphic markers. Marker/trait association analysis revealed that SCYLV resistance in ‘MQ76‐53’ is controlled by Ryl1, a major quantitative trait allele (QTA) accounting for 32% of the phenotypic variance. Ryl1 is the first major QTA described to control a viral disease in sugarcane, and ‘MQ76‐53’ can already be used to introgress SCYLV resistance derived from Ryl1 in elite germplasm.
High production of interleukin‐10 and interferon‐γ in influenza‐associated MERS in the early phase
Authors: Morichi Shinichiro , Kawashima Hisashi , Ioi Hiroaki , Yamanaka Gaku , Kashiwagi Yasuyo , Hoshika Akinori 
Published: July 2012

AbstractMild encephalitis/encephalopathy with a reversible splenial lesion (MERS) occurs in various diseases and pathologies, and the clinical symptoms are not consistent with the impaired region. The mechanism of the region specificity is unclear. We investigated the cytokine profiling in cerebrospinal fluid (CSF) and serum obtained from a child with MERS during influenza infection, and compared them with those of serious another serious type of influenza‐associated encephalopathy. There was no elevation of Interleukin (IL)‐1β, which induces convulsion. The inhibitory cytokines of IL‐10 and IFN‐γ were elevated in the early phase in CSF. Comparing them with other patients, the elevation of the cytokine levels were generally mild. Considering that the prognosis of this MERS case was favorable and high levels of inhibitory cytokines including IL‐10 and IFN‐γ might work to localize the lesion and to prevent sequelae.
Microscopic polyangiitis in a girl with severe anemia and no respiratory symptoms
Authors: Yamato Kazumi , Ishii Takafumi , Kawamura Tomoyuki 
Published: July 2012

AbstractANCA‐positive microscopic polyangiitis is a rare in children. We reported an 11‐year‐old girl without respiratory symptoms with sever anemia which shows signs of hemolytic anemia in test data. Chest X‐ray and computed tomography scans suggested alveolar hemorrhage. The patent developed night hypoxia and moderate proteinuria. Serum MPO‐antibody was highly positive and renal biopsy demonstrated segmental necrosis or crescent formation. A diagnosis of microscopic polyangiitis was made. She responded to pulsed methylprednisolon and pulsed Endoxan therapy. Microscopic polyangitiis should be considered early in the differential diagnosis of patients presenting with anemia of an unknown origin.
Left atrium thrombus in an extremely low‐birthweight infant with late‐onset circulatory dysfunction
Authors: Ina Shihomi , Futatani Takeshi , Higashiyama Hiroyuki , Hashida Nobuko , Fujita Shuhei , Shimura Shoetsu , Igarashi Noboru , Hatasaki Kiyoshi 
Published: July 2012

AbstractA left atrium thrombus, potentially a life‐threatening complication, is an extremely rare in early infancy. Most cases are caused by mal‐placement of central venous catheters or related to congenital heart diseases with left atrial blood congestion. Here we present an extremely low birth weight infant who developed a left atrial thrombus during the course of late onset circulatory dysfunction. The thrombus was successfully treated by recombinant tissue plasminogen activator. A hemodynamically unstable condition like late onset circulatory dysfunction should be taken into consideration as a potential risk condition of this rare disease.
DESCARTES' ‘PROVISIONAL MORALITY’
Authors: CIMAKASKY JOSEPH , POLANSKY RONALD 
Published: July 2012

AbstractDiscourse on Method part 3 offers une morale par provision, usually translated as ‘a provisional moral code’. Occasionally it has been questioned that this code is temporary and restricted to those engaged in pure inquiry. We argue that Descartes intends the moral code to be his final ethical position universally applicable. Since the moral code is ‘derived from’ the rules of method, it should have their permanence, holding for the time pure inquiry commences and when it completes the sciences. Moreover, the four moral maxims replace the classical cardinal virtues. Thus they are meant to govern the lives of all persons.
Expression of chondroitin‐glucuronate C5‐epimerase and cellular immune responses in patients with hepatocellular carcinoma
Authors: Mizukoshi Eishiro , Fushimi Kazumi , Arai Kuniaki , Yamashita Tatsuya , Honda Masao , Kaneko Shuichi 
Published: July 2012

AbstractBackground & AimsChondroitin‐glucuronate C5‐epimerase is an enzyme that converts D‐glucuronic acid to L‐iduronic acid residues in dermatan sulphate biosynthesis. It is also identified to be a tumour‐associated antigen recognized by cytotoxic T cells (CTLs) and its enhanced expression in many cancers has been reported. In the present study, we investigated the usefulness of this molecule as an immunotherapeutic target in hepatocellular carcinoma (HCC).MethodsThe expression of chondroitin‐glucuronate C5‐epimerase in hepatoma cell lines and HCC tissues was confirmed by immunofluorescence and immunohistochemical analysis. CTL responses were investigated by several immunological techniques using peripheral blood mononuclear cells (PBMCs) or tumour‐infiltrating lymphocytes. To determine the safety of immunotherapy using chondroitin‐glucuronate C5‐epimerase‐derived peptide, 12 patients with HCC were administered s.c. vaccinations of the peptides and analysed.ResultsChondroitin‐glucuronate C5‐epimerase was expressed in HCC cell lines and human tissues including alpha‐foetoprotein (AFP)‐negative individuals. Chondroitin‐glucuronate C5‐epimerase‐specific CTLs could be generated by stimulating PBMCs of HCC patients with peptides and they showed cytotoxicity against HCC cells expressing the protein. The frequency of CTL precursors investigated by enzyme‐linked immunospot (ELISPOT) assay was 0–34 cells/3 × 105 PBMCs and the infiltration of interferon‐gamma‐producing CTLs into the tumour site was confirmed. In the vaccination study, no severe adverse events were observed and the peptide‐specific CTLs were induced in 4 of 12 patients tested.ConclusionsChondroitin‐glucuronate C5‐epimerase is a potential candidate for tumour antigen with immunogenicity and the peptides derived from this antigen could be useful in HCC immunotherapy.
High‐Risk Electrocardiographic Parameters are Ubiquitous in Patients with Ischemic Cardiomyopathy
Authors: Carey Mary G. , Al‐Zaiti Salah S. , Canty John M. , Fallavollita James A. 
Published: July 2012

Background: The electrocardiogram (ECG) can be used to predict cardiovascular risk; however, like all risk factors with imperfect specificity, studies in low risk populations have been plagued by poor predictive accuracy. Although predictive accuracy might be improved among cohorts with a higher likelihood of cardiovascular events, this would also affect the prevalence of abnormal parameters and their exclusions.Method: To determine the magnitude of these changes in a cohort with ischemic cardiomyopathy we analyzed 15 previously validated high‐risk parameters from the resting and ambulatory ECG in subjects enrolled in the Prediction of Arrhythmic Events with Positron Emission Tomography (PAREPET) study (n = 198).Results: Using the published exclusion criteria from the validation studies (i.e., atrial fibrillation, persistent pacing, prolonged QRS), only 4 high‐risk ECG parameters (27%) could be evaluated in all subjects and only 42% of subjects could have all 15 ECG parameters assessed. Nevertheless, almost every subject (97%) had at least one abnormal parameter. On average, there were 3.4 ± 1.8 (range, 0–8) high‐risk ECG parameters per subject among the 11.7 ± 4.5 (range, 4–15) parameters that could be assessed.Conclusions: Thus, 34% of all assessable parameters were abnormal. In conclusion, a significant proportion of ECG parameters cannot be assessed in patients with ischemic cardiomyopathy, but high‐risk results are ubiquitous. The influence of these issues will be clarified when the results of the PAREPET study are available to actually determine the predictive value of these parameters on cause‐specific mortality in a high‐risk cohort.
Relation between Beat‐to‐Beat QT Interval Variability and T‐Wave Amplitude in Healthy Subjects
Authors: Hasan Muhammad A. , Abbott Derek , Baumert Mathias 
Published: July 2012

Objectives: Elevated beat‐to‐beat QT interval variability (QTV) has been associated with increased cardiovascular morbidity and mortality.The aim of this study was to investigate interlead differences in beat‐to‐beat QTV of 12‐lead ECG and its relationship with the T wave amplitude.Methods: Short‐term 12‐lead ECGs of 72 healthy subjects (17 f, 38 ± 14 years; 55 m, 39 ± 13 years) were studied. Beat‐to‐beat QT intervals were extracted separately for each lead using a template matching algorithm. We calculated the standard deviation of beat‐to‐beat QT intervals as a marker of QTV as well as interlead correlation coefficients. In addition, we measured the median T‐wave amplitude in each lead.Results: There was a significant difference in the standard deviation of beat‐to‐beat QT intervals between leads (minimum: lead V3 (2.58 ± 1.36 ms), maximum: lead III (7.2 ± 6.4 ms), ANOVA: P < 0.0001). Single measure intraclass correlation coefficients of beat‐to‐beat QT intervals were 0.27 ± 0.18. Interlead correlation coefficients varied between 0.08 ± 0.33 for lead III and lead V1 and 0.88 ± 0.09 for lead II and lead aVR. QTV was negatively correlated with the T‐wave amplitude (r =–0.62, P < 0.0001). There was no significant affect of mean heart rate, age or gender on QT variability (ANOVA: P > 0.05).Conclusions: QTV varies considerably between leads in magnitude as well as temporal patterns. QTV is increased when the T wave is small.
Risk Stratification for Serious Arrhythmic Events Using Nonsustained Ventricular Tachycardia and Heart Rate Turbulence Detected by 24‐Hour Holter Electrocardiograms in Patients with Left Ventricular Dysfunction
Authors: Miwa Yosuke , Yoshino Hideaki , Hoshida Kyoko , Miyakoshi Mutsumi , Tsukada Takehiro , Yusu Satoru , Ikeda Takanori 
Published: July 2012

Background: Previous studies have described the clinical usefulness of the presence of nonsustained ventricular tachycardia (NSVT) and defined heart rate turbulence (HRT) in stratifying patients at risk. We prospectively assessed whether HRT can facilitate the predictive accuracy of NSVT for identifying patients at risk for serious arrhythmic events in patients with left ventricular (LV) dysfunction.Methods: We enrolled 299 consecutive patients with LV dysfunction (ejection fraction ≤ 40%) including ischemic (n = 184) and nonischemic causes (n = 115). The presence of NSVT was assessed on Holter electrocardiograms (ECGs). HRT was simultaneously measured from Holter ECGs, assessing two parameters: turbulence onset (TO) and turbulence slope (TS). HRT was considered positive when both TO and TS were abnormal. The end point was defined as of sudden cardiac death (SCD) and sustained ventricular tachyarrhythmias (VTs).Results: NSVT was documented in 93 patients (32.7%). For HRT assessment, 17 patients (5.6%) were not utilized. Of 282 patients, 68 (24.1%) were HRT positive. During follow‐up of 960 ± 444 days, 14 patients (5.0%) reached the end point. NSVT, HRT, and diabetes were significantly associated with the end point. On multivariate analysis, NSVT had  the strongest value for the end point, with an HR of 4.4 (95%CI, 1.4–14.3; P = 0.0138). When NSVT combined with HRT, the predictive accuracy is more increased, with an HR of 8.2 (95%CI, 2.9–23.3; P < 0.0001). The predictive values of the combination were higher than single use of NSVT or HRT.Conclusions: HRT can facilitate the predictive accuracy of NSVT for identifying patients at risk for serious arrhythmic events in patients with LV dysfunction.
Optimal QT/JT Interval Assessment in Patients with Complete Bundle Branch Block
Authors: Chiladakis John , Kalogeropoulos Andreas , Koutsogiannis Nikolaos , Zagkli Fani , Vlassopoulou Niki , Chouchoulis Konstantinos , Alexopoulos Dimitrios 
Published: July 2012

Background: Prolonged ventricular repolarization duration confers increased risk for malignant ventricular arrhythmias. We sought to clarify the optimal method of QT/JT interval assessment in patients with complete bundle branch block (BBB).Methods: Study patients (n = 71) were dual‐chamber device recipients with baseline left or right BBB who preserved intrinsic ventricular activation during incremental atrial pacing. Patients were classified according to the presence or not of structural heart disease. The former group received chronic amiodarone therapy. QT and JT intervals were recorded at baseline heart rate of 51 ± 4 beats/min and during atrial pacing at 60, 80, and 100 beats/min. We used linear mixed‐effects models to assess the effect of heart rate on the derived QTc and JTc values with the use of six different heart rate correction formulae.Results: Heart rate had a significant effect on the QTc and the JTc intervals regardless of the correction formula used (P < 0.001 for all formulae). The formula of Hodges demonstrated the least variability in QTc and JTc measurements across the different heart rates in both patients groups without (F = 15.05 and F = 13.53, respectively) and with structural heart disease (F = 5.71 and F = 7.69, respectively), followed by the Nomogram and Framingham methods, whereas the uncorrected QT and JT intervals showed comparable heart rate–dependency. The application of Bazett's JTc and QTc led to the most pronounced interval variations in any case with BBB.Conclusions: The Hodges, Nomogram and Framingham correction methods provide best assessment of QT/JT intervals in BBB, whereas Bazett's formula exaggerates heart rate–dependency of ventricular repolarization intervals.
Identification of two novel HLA‐A alleles: A*24:199 and A*02:324
Authors: Mantovani M. , Frison S. , Longhi E. , Tagliaferri C. , Mantia M. , Piccolo G. , Poli F. 
Published: July 2012

SummaryHere, we describe two new HLA‐A alleles: A*24:199 and A*02:324. The two new variants are attributed to a single nucleotide mutation namely A→C for A*24:199 and G→A for A*02:324. Both point mutations are responsible for a change in translated amino acids.
Analysis of genetic diversity in the bioenergy plant Erianthus arundinaceus (Poaceae: Andropogoneae) using amplified fragment length polymorphism markers
Authors: Tsuruta Shin‐ichi , Ebina Masumi , Kobayashi Makoto , Hattori Taiichiro , Terauchi Takayoshi 
Published: July 2012

AbstractThe genetic diversity among 30 accessions including 18
Erianthus arundinaceus and 12
Saccharum species was analyzed using amplified fragment length polymorphism (AFLP) markers. We obtained 2245 fragments using 16 primer combinations. The
Erianthus collection generated 1115 fragments of which 772 (69.2%) were polymorphic. Of the 342 monomorphic fragments (30.8%), 222 were specific to
E
. arundinaceus. The neighbor‐joining tree based on the detected fragments clearly separated the
Erianthus and
Saccharum accessions into distinct groups. The
Erianthus collection was further subdivided into two Japanese groups and one Indonesian group, with a high level of genetic differentiation among these groups (
F

st = 0.2062). These results represent the first molecular characterization of the Japanese
E. arundinaceus breeding materials and provide a useful initial resource for breeding to improve the bioenergy potential of
Erianthus spp.
A large‐scale gene discovery for the red palm weevilRhynchophorus ferrugineus(Coleoptera: Curculionidae)
Authors: Wang Lei , Zhang Xiao‐Wei , Pan Lin‐Lin , Liu Wan‐Fei , Wang Da‐Peng , Zhang Guang‐Yu , Yin Yu‐Xin , Yin An , Jia Shan‐Gang , Yu Xiao‐Guang , Sun Gao‐Yuan , Hu Song‐Nian , Al‐Mssallem Ibrahim S. , Yu Jun 
Published: July 2012

AbstractThe red palm weevil (RPW;Rhynchophorus ferrugineus) is a devastating pest of palms, prevalent in the Middle East as well as many other regions of the world. Here, we report a large‐scalede novocomplementary DNA (cDNA) sequencing effort that acquired ∼5 million reads and assembled them into 26 765 contigs from 12 libraries made from samples of different RPW developmental stages based on the Roche/454 GS FLX platform. We annotated these contigs based on the publically available known insect genes and theTribolium castaneumgenome assembly. We find that over 80% of coding sequences (CDS) from the RPW contigs have high‐identity homologs to known proteins with complete CDS. Gene expression analysis shows that the pupa and larval stages have the highest and lowest expression levels, respectively. In addition, we also identified more than 60 000 single nucleotide polymorphisms and 1 200 simple sequence repeat markers. This study provides the first large‐scale cDNA dataset for RPW, a much‐needed resource for future molecular studies.
Retracted: Improvement of Breadmaking Quality by Xylanase GH11 from Penicillium occitanisPol6
Authors: Driss Dorra , Bhiri Fatma , Siela Mariem , Bessess Souhail , Chaabouni Semia , Ghorbel Raoudha 
Published: July 2012

AbstractIn this study xylanase from Penicillium occitanis Pol6, as a bread improver, was tested in whole‐wheat bread. The purified xylanase termed PoXyn2 was used as an additive at a very small quantity (0.12 U/g flour basis) during mixing of wheat flour. The effects of xylanase addition on the fermentation stage and the final bread quality were analyzed. Remarkable decrease (8%) in water absorption and increase in dough rising (36.8%) were noticed. Final moisture content of the bread was higher (37.50%) than control (23.56%). Improvements in volume (17.8%) and specific volume (34.9%) were also significant. Sensory evaluation indicated better flavor, taste, softness and overall acceptability. Texture profile analysis confirmed the rheological changes. Firmness was decreased by more than twofolds. Improvements in cohesiveness and decline in springiness and gumminess were observed.Practical ApplicationsThe baking industry is shifting toward producing whole‐wheat fiber‐rich bread using enzymes such as xylanase, which can improve dough machinability and stability, oven spring, loaf volume, crumb structure and shelf life. This hypothesis is supported by determining the functional and rheological properties of supplemented dough with purified Penicillium occitanis xylanase. The improved bread was assessed by a panel of five judges for sensory evaluation. Furthermore, significant improvement in textural properties was noticed.
Cobalamin status in sickle cell disease
Authors: Ajayi O. I. , Bwayo‐Weaver S. , Chirla S. , Serlemitsos‐Day M. , Daniel M. , Nouraie M. , Edwards K. , Castro O. , Lombardo F. , Gordeuk V. R. 
Published: July 2012

SummaryIntroductionSome studies comparing serum cobalamin in individuals with and without sickle cell disease (SCD) have suggested a higher prevalence of cobalamin deficiency in SCD but others have not. Our aim was to prospectively compare cobalamin status in African–Americans with and without SCD.MethodsWe analyzed blood samples from 86 subjects in two groups: SCD (n = 29) and non‐SCD (n = 57). Serum cobalamin, folate, homocysteine, methylmalonic acid (MMA), anti‐intrinsic factor antibody,
Helicobacter pylori antibody, and gastrin were measured and compared.ResultsThe median cobalamin was 235 pm in the SCD group vs. 292 pm in the non‐SCD group (P‐value = 0.014). No significant differences in MMA or homocysteine were seen. Using the criteria of a low cobalamin and an elevated MMA or an elevated MMA alone, cobalamin deficiency was suggested in 4 (13.8%) in the SCD group and 6 (10.5%) in the non‐SCD group. Two of these SCD patients and four of these control subjects had chronic renal disease, which may lead to elevated MMA in the absence of cobalamin deficiency. The remaining four met criteria for cobalamin deficiency, 2 (6.9%) in the SCD group and 2 (3.5%) in the non‐SCD group (P = 0.6).ConclusionA lower cobalamin was observed in SCD patients without a higher prevalence of cobalamin deficiency. The inclusion of haptocorrin and holotranscobalamin measurement in future studies may provide a better assessment of cobalamin status in this patient group.