bluuebunny/crossref_metadata_2025_split huggingface dataset:
Modulation of Calcium Signaling on Demand to Decipher the Molecular Mechanisms of Primary Aldosteronism
Authors: Fedlaoui Bakhta , Cosentino Teresa , Al Sayed Zeina R. , Alexandre Coelho Rita , Giscos-Douriez Isabelle , Faedda Nicolo , Fayad May , Hulot Jean-Sebastien , Magnus Chris , Sternson Scott M. , Travers-Allard Simon , Baron Stephanie , Penton David , Fernandes-Rosa Fabio L. , Zennaro Maria-Christina , Boulkroun Sheerazed 
Published: February 2025

BACKGROUND:
Primary aldosteronism is the most common form of secondary hypertension. The most frequent genetic cause of aldosterone-producing adenomas is somatic mutations in the potassium channel KCNJ5. They affect the ion selectivity of the channel, with sodium influx leading to cell membrane depolarization and activation of calcium signaling, the major trigger for aldosterone biosynthesis.

METHODS:

To investigate how
KCNJ5
mutations lead to the development of aldosterone-producing adenomas, we established an adrenocortical cell model in which sodium entry into the cells can be modulated on demand using chemogenetic tools (H295R-S2 α7-5HT3-R [α7-5HT3 receptor] cells). We investigated their functional and molecular characteristics with regard to aldosterone biosynthesis and cell proliferation.

RESULTS:

A clonal cell line with stable expression of the chimeric α7-5HT3-R in H295R-S2 cells was obtained. Increased sodium entry through α7-5HT3-R upon stimulation with uPSEM-817 led to cell membrane depolarization, opening of voltage-gated Ca
2+
channels, and increased intracellular Ca
2+
concentrations, resulting in the stimulation of
CYP11B2
expression and increased aldosterone biosynthesis. Increased intracellular sodium influx did not increase proliferation but rather induced apoptosis. RNA sequencing and steroidome analyses revealed unique profiles associated with Na
+
entry, with only partial overlap with Ang II (angiotensin II) or potassium-induced changes.

CONCLUSIONS:

H295R-S2 α7-5HT3-R cells are a new model reproducing the major features of cells harboring
KCNJ5
mutations. Increased expression of
CYP11B2
and stimulation of the mineralocorticoid biosynthesis pathway are associated with a decrease of cell proliferation and an increase of apoptosis, indicating that additional events may be required for the development of aldosterone-producing adenomas.
Greens of a Different Shade? The Greek Party for the Animals
Authors: Botetzagias Iosif , Tavridis George 
Published: February 2025

Abstract
Available research has established that animal advocacy parties, albeit constituting a distinctive party family, have many similarities with green parties. However, this assessment has been based on analyses of animal advocacy parties’ political/election manifestos. In this research, we revisit this question for the youngest European animal advocacy party, the Greek Komma gia ta Zoa (KZ, Party for the Animals, established in 2020), through an analysis of the party members’ views. We find that the members have a strong left-libertarian agenda and worldview. Nevertheless, their lesser concern about trademark environmental issues suggests that they indeed differ from green parties.
Seeing is Believing: Repeated Exposure to Animals in Anthropomorphic Contexts Promotes Desire for Exotic Pets
Authors: Castaneda Marielle C. , Caine Nancy G. 
Published: February 2025

Abstract
Research has shown that visual depictions of nonhuman animals in anthropomorphic settings can foster beliefs that wild animals make good pets and are safe in the wild. These beliefs may develop due to repeated exposure to anthropomorphic images, but no study has experimentally demonstrated this. Slideshows in which the ratio of anthropomorphic images to natural images varied for three nonhuman animal species were created. After viewing one of the slideshows, participants rated the species’ “pet suitability” and endangerment status. People who saw mostly anthropomorphic images reported higher pet suitability than those who saw equal numbers of the two image types, followed by those who saw mostly natural images. This study is the first to confirm that with increasing exposure to anthropomorphic images, people become more convinced that wild animals make good pets, an opinion that affects animal conservation and welfare by supporting the exotic pet trade.
Human Rights: a Sophiological Reenvisioning
Authors: Gallaher Brandon 
Published: February 2025

Abstract
The article proposes a new sophiological reading of the human rights tradition which attempts to understand how the theology of Holy Wisdom might transform what we understand about the broad theological discourse on human rights by grounding those rights within not only theological anthropology but Trinitarian theology. After touching briefly on a few examples of more negative Orthodox Christian viewpoints on human rights (Christos Yannaras and the official teaching of the Russian Orthodox Church, Moscow Patriarchate), the study argues that there also existed a movement from the late 19th to the mid-20th centuries of Russian Orthodox religious thinkers in the neo-idealist and Russian liberal tradition that articulated a broad based and positive Christian humanism that upheld a vision of personhood, human dignity, human rights and natural law within the context of divinisation (theosis). Drawing on some of these thinkers, especially those in the sophiological tradition (including Vladimir Solov’ev, Sergii Bulgakov and Semyon Frank), and drawing links with Jacques Maritain, it looks successively at the following issues: human personhood as a sacral uniqueness and dignity found within the image and likeness of God and this being the foundation of human rights as an inviolable creative capacity seen above all in freedom of conscience; that human rights and freedom of conscience are based on natural law and this comes from the eternal law which is grounded not only in creaturely Wisdom but also in the Divine Wisdom so that we may say that the image and likeness of God is a “sophianic image” reflecting the Divine Sophia and that in God as Trinity, by extension, one encounters the image of the human being; that there is an organic and preeternal Trinitarian link between natural law and human rights and that we can see this link within the logoi spermatikoi or seminal words tradition as found within the thinking of Basil the Great and Maximus the Confessor; and human rights are not merely a capacity for creativity, right as the power of freedom, but that rights can be understood as a calling or vocation of humanity for perfectibility, by which is understood divinization (theosis). The essay concludes with words affirming not only the importance of defending human rights in order to protect the marginalized or to stand against tyranny but the need to have a wholistic Orthodox theological vision of rights as being nestled within the life of the holy Trinity with human rights understood as both an inviolable creative capacity but also this potentiality being a calling and obligation to divinize the world.
A Discussion on Internal Repetition among Liu Yong’s Ci Poems and Its Effects on Their Transmission
Authors: Wang Chao 
Published: February 2025

Abstract
Internal repetition in literary works is a common occurrence; however, there are differences in the degree in which it appears. There is a high amount of repetition in the wording, themes, and compositional structure of Liu Yong’s 柳永 ci poetry. When considering the creative process, the reasons for repetitive and similar texts stem from influences pertaining to the predetermined audience, the length of time used in creating a work, and revisions. Nonetheless, the repetition in Liu’s ci poems did not have a negative impact on their circulation as would be expected, perhaps because they were consumed as individual pieces circulated as songs, making it so audiences had no means of comparison. In addition, their existence as “auditory texts” diminished the focus on their wording. Furthermore, the innovation of manci poetry and its transmission among different audiences made it so that people of the Song paid little attention to the textual repetition in Liu’s ci poetry. During the early years in which manci gained prominence, the repetition occurring within Liu’s ci poems may have had a positive influence.
Catastrophic Antiphospholipid Syndrome: Clinical Profile and Outcome of Physician Diagnosed CAPS Cases from a North Indian Cohort
Authors: Rahulan Lekshmi Minikumari , Thomas Koshy Nithin , Chatterjee Rudrarpan , Misra Durga Prasanna , Agarwal Vikas , Lawrence Able , Aggarwal Amita 
Published: February 2025

Objective: To describe the clinical profile and outcome of patients with catastrophic antiphospholipid syndrome (CAPS) from a tertiary care Rheumatology centre in North India. Methods: A retrospective analysis of data of patients between 1990 and March 2024 was done. Those with rapid involvement of three or more organ systems with moderate to high titres of antiphospholipid antibodies (aPL) at least once were considered physician-diagnosed CAPS. Results: Fourteen patients were considered as cases of CAPS. The majority were females, with a median age of 28 years (26.25–37.25). Two-thirds of the patients had systemic lupus erythematosus (SLE) as the background illness. Thrombocytopenia was present in all cases, and CAPS was the presenting manifestation in half of them. An identifiable trigger was found in only nine cases predominantly infection. Triple therapy with intravenous immunoglobulin (IVIG), pulse steroids, and heparin was received by six patients, resulting in clinical recovery in five of them. Nine patients received anticoagulation and the rest received glucocorticoids, other immunosuppressive agents and antibiotics for underlying sepsis. Among the five patients who did not receive heparin, three died of multiorgan dysfunction. Two patients died despite anticoagulation owing to late presentation and delay in initiation of heparin. Conclusion: A high index of suspicion should be kept for CAPS, especially in a young female with rapidly progressive multiorgan dysfunction in the background of autoimmune features or pregnancy morbidity. Triple therapy using heparin, IVIG and high-dose steroids should be considered in patients with suspected CAPS.
Auditory Neuropathy Spectrum Disorders: A Scoping Review
Authors: Swain Santosh Kumar , Prusty Venkat Raman 
Published: February 2025

Background and Aims: Auditory Neuropathy Spectrum Disorder (ANSD) is a condition that affects the auditory pathway, specifically the transmission of sound signals from the ear to the brain. In ANSD, the outer hair cells (OHCs) in the cochlea, which are responsible for amplifying sound, typically function normally. However, there is an issue in the transmission of sound signals from the cochlea to the brain. ANSD is often due to problems in the inner hair cells (IHCs), the auditory nerve fibres, or the synaptic connections between them. Prematurity, ototoxic medication therapy, hyperbilirubinemia and positive family history are risk factors for ANSD. Methods: The search for published articles on ANSD was done with databases like PubMed, Scopus, Medline and Google Scholar. This article discusses on prevalence, etiopathogenesis, clinical manifestations, diagnosis and present treatment of ANSD. Results: ANSD encompasses a variety of hearing disorders that are typified by poor speech perception even though pure tone detection thresholds are largely intact. The afflicted patients exhibit speech-discriminating issues that are disproportionate to their pure tone detection thresholds, especially when there is background noise. Acoustic reflexes, otoacoustic emissions (OAEs), cochlear microphonics (CMs) and auditory brainstem evoked responses (ABERs) are missing or significantly reduced in patients with ANSD. While cochlear implants are the primary option for people with substantial hearing loss, especially in cases with IHCs presynaptic diseases or when hearing aids have failed, hearing aids are beneficial for ANSD patients with mild to moderate hearing loss. Conclusion: As OAEs and/or CMs of neonates with ANSD are normal, auditory evoked responses should be added to screening tests for high-risk neonates in order to prevent false-negative results.
Association of lumbar disc herniation and paraspinal muscles changes in patients with chronic low back pain
Authors: Mou Zhanglin , Yi Wenfang , Luo Mingbin , Yang Yingjuan , Wang Zhongwei , He Bo , Zhao Derong 
Published: February 2025

Background Patients with chronic low back pain (CLBP) exhibit changes in paraspinal muscles fat infiltration and cross-sectional area (CSA). However, the relationship between lumbar disc herniation (LDH) and paraspinal muscles changes in CLBP patients remains unclear. Objective To analyze the relationship between LDH, fat infiltration in paraspinal muscles, and their CSA in patients with CLBP. Methods A total of 494 patients (201 males and 293 females) with an average age of 45.75 ± 12.93 years with CLBP were included in this study. Conventional magnetic resonance scanning sequences, inverted phase and Iterative Decomposition of water and fat with the Echo Asymmetry and Least-Square Estimation quantitation (IDEAL-IQ) sequences were used, and patients were assessed for pain on a visual analog scale (VAS). LDH was assessed based on T2 axial images at the L4-S1 level. Multifidus and erector spinae CSA and proton density fat fraction (PDFF) were measured bilaterally at the central level of the L4-S1 disc using the above-mentioned sequences and normalized by the square of height. Results Patients with LDH had a higher PDFF of the paraspinal muscles at the L4/5 level compared to those without LDH ( p < 0.05). Additionally, the VAS score of patients with LDH was significantly higher than that of the control group ( p < 0.05). However, there was no significant difference in the change of paraspinal muscles CSA between patients with CLBP with and without LDH. Conclusion In patients with LDH, there is increased fatty infiltration in the paraspinal muscles, with no significant changes in CSA. Different types of LDH are not related to paraspinal muscles fatty infiltration and CSA.
The Association Between Tumor Burden and the Efficacy of Immunotherapy Among Patients With Non-small Cell Lung Cancer
Authors: Hui Jia-Jun , Yan Han-Lu , Ding Sheng-Jun , Qin Bao-Dong , Jiao Xiao-Dong , Zang Yuan-Sheng 
Published: February 2025

Background This cohort study aimed to evaluate the impact of tumor burden (TB) on the efficacy of immunotherapy in patients with advanced non-small cell lung cancer (NSCLC). Materials and Methods Data from the POPLAR and OAK trials were extracted as the training and validation cohorts, respectively. TB was defined as the sum of the longest dimensions (blSLD) of measurable target lesions as per RECIST v1.1. The Kaplan-Meier curves and multivariate Cox regression analyses were performed to assess the association between TB with blood-tested tumor mutation burden (bTMB), PD-L1 expression, and survival outcomes. Additionally, random forest algorithms analysis was performed to evaluate the accuracy of TB in predicting 12-month mortality of NSCLC patients received atezolizumab. Results A total of 105 patients from the POPLAR trial and 322 patients from the OAK trial were recruited in the training and validation sets, respectively. Patients with TB-L have significantly better OS than those with TB-H in the training (mOS: 15.8 months vs 6.93 months) as well as the validation (mOS: 16.0 months vs 7.59 months) cohort. The multivariate Cox regression analysis indicated that TB is an independent biomarker for OS prediction, regardless of bTMB, PD-L1 expression, and number of metastasis sites. The impact of TB on 12-month mortality was expected to be stronger with the increase of TB, suggesting that patients with a high tumor burden experienced a detrimental effect on 12-month mortality. Conclusion TB may act as a prognostic biomarker for clinical benefit in NSCLC patients treated with immunotherapy alone. This may be potentially effective for predicting the efficacy of immunotherapy-based regimens.
Association of baseline inflammatory biomarkers and clinical outcomes in patients with advanced renal cell carcinoma treated with immune checkpoint inhibitors
Authors: Yildirim Ahmet , Wei Mengting , Liu Yuan , Nazha Bassel , Brown Jacqueline T. , Carthon Bradley C. , Choi Yujin , Suh Lauren , Goswamy Rohit V. , McClintock Greta R. , Hartman Caitlin , Caulfield Sarah , Ciuro Jordan , Goldman Jamie M. , Harris Wayne B. , Kucuk Omer , Master Viraj A. , Bilen Mehmet A. 
Published: February 2025

Background: Immune checkpoint inhibitors (ICIs) have become the mainstay treatment of metastatic kidney cancer, demonstrating enhanced outcomes and durable responses in select patient subgroups. However, identifying reliable prognostic biomarkers for treatment outcomes remains challenging. Objectives: This study aimed to assess the correlation between baseline inflammatory markers and overall survival (OS), progression-free survival (PFS), and clinical benefit (CB) in metastatic kidney cancer patients receiving ICIs. CB was defined as patients achieving stable disease, partial response, or complete response. Design: Retrospective, single-center study. Methods: A retrospective analysis was conducted on 401 adult patients with advanced kidney cancer treated with ICIs at Emory Winship Cancer Institute between 2018 and 2023. Modified Glasgow Prognostic Score (mGPS), neutrophil-to-lymphocyte (NLR), monocyte-to-lymphocyte (MLR), platelet-to-lymphocyte (PLR), and neutrophil-to-eosinophil ratios (NER) were collected from baseline blood samples. Results: Among 401 patients (median age, 66; 71% male; 21% Black/African American), median follow-up was 43.0 months (95% CI, 36.6–51.4). Patients with mGPS scores of 0 had longer OS than those with a score of 1 (hazard ratio (HR), 0.38; 95% CI, 0.23–0.62; p < 0.001) and 2 (HR, 0.37; 95% CI, 0.20–0.67; p = 0.001), and longer PFS compared to patients with mGPS scores of 1 (HR, 0.66; 95% CI, 0.44–0.98; p = 0.039) and 2 (HR, 0.44; 95% CI, 0.29–0.67; p < 0.001). Low baseline NLR was associated with longer PFS (HR, 0.73; 95% CI, 0.54–0.97; p = 0.032). Low baseline MLR correlated with improved OS (HR, 0.60; 95% CI, 0.44–0.83; p = 0.002) and PFS (HR, 0.73; 95% CI, 0.55–0.97; p = 0.031). Similarly, low baseline PLR was associated with higher CB likelihood (odds ratio (OR), 2.20; 95% CI, 1.31–3.69; p = 0.003), and low baseline NER was linked to improved OS (HR, 0.63; 95% CI, 0.46–0.87; p = 0.004), PFS (HR, 0.67; 95% CI, 0.51–0.88; p = 0.003), and higher CB (OR, 2.04; 95% CI, 1.20–3.46; p = 0.008). Conclusion: Lower levels of systemic inflammatory markers are associated with more favorable clinical outcomes with ICI treatment. Prospective studies are needed for further validation.
POCUS using the neo-ECHOTIP protocol plus the retract, advance, and position (RAP) technique to improve lower extremity catheter positioning in neonates
Authors: Ostroff M , Abu-Ali J , DeBari V , Kiblawi F , Zauk A , Datta-Bhutada S , Pua Z 
Published: February 2025

Background International guidelines recommend point-of-care ultrasonography (POCUS) in neonatology for diagnostic, therapeutic, and procedural purposes. However, there are few standard protocols for using POCUS to confirm lower extremity catheter tip positioning. Methods In this prospective study, subcutaneously tunneled femorally inserted central catheters (ST-FICCs) were placed in eligible neonates according to physician orders. In line with the Neo-ECHOTIP (NE) protocol, a subcostal longitudinal view was used to identify the catheter, and saline flush test (SFT) was performed to confirm catheter tip placement below the right atrium. During the study, a novel “retract, advance, and position (RAP)” technique was added to the existing NE-SFT protocol to improve catheter positioning. Two-view radiographs of the chest and abdomen were obtained to verify catheter tip position for all subjects. The agreement rate with post-procedural radiographs and the total number of radiographs needed to achieve proper catheter positioning were assessed. Results The agreement rate of POCUS with radiographs for catheter terminal tip position was 75% (39/52) for the NE-SFT group compared to 100% (48/48) for the NE-SFT + RAP group ( p < 0.001). Catheters in the NE-SFT group required repositioning (25%); no malpositioned catheters were observed in the NE-SFT + RAP group. Repositioning of the catheter in the NE-SFT group resulted in 22 repeat radiographs in the NE-SFT group; none were required in the NE-SFT + RAP group ( p < 0.0001). Conclusions This single-center study demonstrated that applying the RAP technique to the Neo-ECHOTIP protocol improved the ease and accuracy of lower extremity catheter tip position localization in neonates, thus eliminating the use of radiography.
Effects of neck-exercise and health promotion on headache outcomes in office workers: secondary analysis of the NEXpro stepped wedge cluster randomised controlled trial
Authors: Ernst Markus J. , Meichtry André , Luedtke Kerstin ,   the NEXpro collaboration group, Aegerter Andrea , Ajeti Aulona , Barbero Marco , Brunner Beatrice , Buob Samira , Cornwall Jon , Da Cruz Yara , Deforth Manja , Distler Oliver , Dratva Julia , Dressler Holger , Egli Tobias , Elfering Achim , Etzer-Hofer Irene , Felder Salome , Furrer Ramona , Gemperle David , Gisler Michelle , Grob Sandro , Haas Michelle , Holzer Tabea , Hug Delia , Johnston Venerina , Klaus Sandro , Kobelt Gina M. , Luomajoki Hannu , Melloh Markus , Nicoletti Corinne , Niggli Seraina , Nüesch Andrea , Nüssle Achim , Ribeli Kristina , Richard Salome , Sax Nadine , Schmid Monika , Schülke Katja , Siebeneicher Rebecca , Sjøgaard Gisela , Staub Lukas , Störi Seraina , Volken Thomas , Wagner Josephine , Wartmann Ellen , Zweig Thomas , Falla Deborah 
Published: February 2025

Abstract

Background
Headache conditions have a high prevalence worldwide. Office workers with high and demanding workload, but low physical activity levels are considered vulnerable for suffering from headache. This analysis examines whether exercise combined with health promotion at the workplace is effective for headache relief in office workers.

Methods
This study reports the results of secondary outcomes of a stepped wedge cluster randomized controlled trial. Office workers (n = 120) were randomly assigned to a twelve-week supervised intervention period, consisting of neck and shoulder girdle exercises with health promotion interventions performed at the workplace. Secondary outcomes were analysed and modelled for headache occurrence, frequency, and the Headache Impact Test-6 (HIT-6), accounting for possible effects for the intervention, the period it had been introduced, and interactional and nested effects.

Results
At baseline, 88 of the 120 participants reported ≥ one headache episode in the past four weeks, with a mean headache frequency of 3.58 days for that period. The mean HIT-6 score for the entire cohort amounted to 53.6 points. For headache occurrence and HIT-6, the simplest model with the intervention only, showed the best statistical fit with an odds ratio for headache occurrence of 0.46 (95% confidence interval: 0.25 to 0.84), and − 2.23 (95% confidence interval: -3.35 to -1.12) points on the HIT-6 questionnaire. For headache frequency, the model accounting for interaction effects (intervention x period) had the best statistical fit and showed an incidence rate ratio of 0.57 (95% confidence interval: 0.44 to 0.74) for the first period, but not for later ones.

Conclusions
Neck exercises and health promotion had a positive impact on headache occurrence, headache frequency and HIT-6, with the latter not reaching clinical importance. Although only statistically significant for headache frequency, larger effects were found during earlier periods or shorter interventional exposure for all outcomes, necessitating refresher sessions at later periods.

Trial registration
NCT04169646.