bluuebunny/crossref_metadata_2025_split huggingface dataset:
Kinematic History and Changes in the Tectonic Stress Regime during the Cenozoic along the Qinling and Southern Tanlu Fault Zones
Authors: Yueqiao ZHANG , Vergèly Pierre , Mercier Jacques‐Louis , Yongmin WANG , Yong ZHANG , Dezhi HUANG 
Published: October 2010

AbstractSince the mid‐late Eocene, North China has been subjected to extensional stress, resulting in the formation and development of basins. The dynamic origin of this crustal extension has long been an issue of debate. This paper presents the results of kinematic analyses of faults obtained from two seperated areas in North China. In the Weihe graben situated on the southernmost margin of the Ordos block, analyses of fault kinematics were coupled with an analysis of the basin's subsidence history. Three successive extensional tectonic phases accompaning the basin's formation and development have been distinguished. The Palaeogene extension was oriented in a WNW‐ESE direction; the Neogene extension in a NE‐SW direction and the Pliocene‐Quaternary extension in a NW‐SE direction. Such changes have also been recorded by fault kinematics along the southern Tanlu fault zone. This has been demonstrated by three successive sets of fault striations indicating normal dip slip resulting from NW‐SE extension, then left‐lateral slip with a normal component resulting from NE‐SW extension, and right‐lateral slip with a minor normal component, respectively. The kinematic history of faults and their chronological evolution indicate changes in continental dynamics acting in North China over Cenozoic time.
Grey Prediction of Seismic Activity in the Three Gorges Area of the Yangtze River
Authors: Daogong HU , Shuren WU , Chengxuan TAN 
Published: October 2010

AbstractOn the basis of historical earthquake data and recent observations, two grey models prediction of the next seismic active period and the maximal magnitude of earthquakes which might occur during that period are constructed on the basis of the grey theory. As predicted by the two models, the next seismic active period would last 79 years, and that the maximal magnitude of earthquakes occurring within the period would not exceed 5.2. These results provide an important basis for the assessment of earthquake risks and reduction of disasters in the Three Gorges area.
Geomagnetic Polarity Events in China since 2.48 Ma
Authors: Tongming GE , Jian LIU , Limin FAN 
Published: October 2010

AbstractThis paper presents the results of palaeomagnetic studies of Cenozoic volcanic rocks and Quaternary strata in China, which have confirmed the occurence of some short‐period polarity events. After a correlation with the worldwide palaeomagnetic data, a geomagnetic polarity time scale for the time since 2.48 M*** has been compiled.
Specific Control of Pseudomonas aeruginosa Surface-Associated Behaviors by Two c-di-GMP Diguanylate Cyclases
Authors: Merritt Judith H. , Ha Dae-Gon , Cowles Kimberly N. , Lu Wenyun , Morales Diana K. , Rabinowitz Joshua , Gitai Zemer , O’Toole George A. 
Published: October 2010

ABSTRACT

The signaling nucleotide cyclic diguanylate (c-di-GMP) regulates the transition between motile and sessile growth in a wide range of bacteria. Understanding how microbes control c-di-GMP metabolism to activate specific pathways is complicated by the apparent multifold redundancy of enzymes that synthesize and degrade this dinucleotide, and several models have been proposed to explain how bacteria coordinate the actions of these many enzymes. Here we report the identification of a diguanylate cyclase (DGC), RoeA, of
Pseudomonas aeruginosa
that promotes the production of extracellular polysaccharide (EPS) and contributes to biofilm formation, that is, the transition from planktonic to surface-dwelling cells. Our studies reveal that RoeA and the previously described DGC SadC make distinct contributions to biofilm formation, controlling polysaccharide production and flagellar motility, respectively. Measurement of total cellular levels of c-di-GMP in ∆r
oeA
and ∆
sadC
mutants in two different genetic backgrounds revealed no correlation between levels of c-di-GMP and the observed phenotypic output with regard to swarming motility and EPS production. Our data strongly argue against a model wherein changes in total levels of c-di-GMP can account for the specific surface-related phenotypes of
P. aeruginosa
.

IMPORTANCE
A critical question in the study of cyclic diguanylate (c-di-GMP) signaling is how the bacterial cell integrates contributions of multiple c-di-GMP-metabolizing enzymes to mediate its cognate functional outputs. One leading model suggests that the effects of c-di-GMP must, in part, be localized subcellularly. The data presented here show that the phenotypes controlled by two different diguanylate cyclase (DGC) enzymes have discrete outputs despite the same total level of c-di-GMP. These data support and extend the model in which localized c-di-GMP signaling likely contributes to coordination of the action of the multiple proteins involved in the synthesis, degradation, and/or binding of this critical signal.
To refer or not?
Authors: Stevenson Michael 
Published: October 2010

THIS series gives readers the opportunity to consider and contribute to discussion of some of the ethical dilemmas that can arise in veterinary practice. Each month, a case scenario is presented, followed by discussion of some of the issues involved. In addition, a possible way forward is suggested; however, there is rarely a cut‐anddried answer in such cases, and readers may wish to suggest an alternative approach. This month's dilemma, ‘To refer or not?’, is presented and discussed by Michael Stevenson. Readers with comments to contribute are invited to send them as soon as possible, so that they can be considered for publication in the next issue. Discussion of the dilemma ‘Disbudding illegally?’, which was published in the February issue of In Practice, appears on page 123.The series is being coordinated by Siobhan Mullan, of the University of Bristol. It is hoped it will provide a framework that will help practices find solutions when facing similar dilemmas.
Everyday ethics
Authors: Anzuino Kathy 
Published: October 2010

THIS?series gives readers the opportunity to consider and contribute to discussion of some of the ethical dilemmas that can arise in veterinary practice. Each month, a case scenario is presented, followed by discussion of some of the issues involved. In addition, a possible way forward is suggested; however, there is rarely a cut‐anddried answer in such cases, and readers may wish to suggest an alternative approach. This month's dilemma, ‘Disbudding illegally?’, was submitted by a reader and is presented and discussed by Clare Main. Readers with comments to contribute are invited to send them as soon as possible, so that they can be considered for publication in the next issue. Discussion of the dilemma ‘Postoperative responsibility’, which was published in the January issue of In Practice, appears on page 79.The series is being coordinated by Siobhan Mullan, of the University of Bristol. It is hoped it will provide a framework that will help practices find solutions when facing similar dilemmas.
Can't pay, won't pay?
Authors: Barker Chris 
Published: October 2010

THIS series gives readers the opportunity to consider and contribute to discussion of some of the ethical dilemmas that can arise in veterinary practice. Each month, a case scenario is presented, followed by discussion of some of the issues involved. In addition, a possible way forward is suggested; however, there is rarely a cut‐anddried answer in such cases, and readers may wish to suggest an alternative approach. This month's dilemma, ‘Can't pay, won't pay?’, is presented and discussed by Chris Barker. Readers with comments to contribute are invited to send them as soon as possible, so that they can be considered for publication in the next issue. Discussion of the dilemma ‘TB testing protocol’, which was published in the July/August issue of In Practice, appears on pages 414 to 415.The series is being coordinated by Siobhan Mullan, of the University of Bristol. It is hoped it will provide a framework that will help practices find solutions when facing similar dilemmas.
Vitamin D status and recommendations to improve vitamin D status in Canadian youth
Authors: Mark Sean 
Published: October 2010

Little is known regarding the vitamin D status of Canadian youth. Our objectives were (i) to describe the vitamin D status of Quebec youth using a representative sample; (ii) to examine the relative contributions of diet, physical activity, and fat mass to the variance in plasma 25-hydroxyvitamin D(25(OH)D), the best biomarker of vitamin D status; and (iii) to examine the influence of household income and food insecurity on the intakes of dietary vitamin D, calcium, and dairy foods. To describe vitamin D status, we used data from the Quebec Child and Adolescent Health and Social Survey (QCAHS), which is a cross-sectional survey representative of Quebec youth aged 9, 13, and 16 years. For the second objective, 159 youth, aged 8 to 11 years, whose parents (at least one) were obese or had the metabolic syndrome, were used for cross-sectional analysis in the Quebec Adipose and Lifestyle InvesTigation in Youth (QUALITY). Fat mass was measured using dual X-ray absorptiometry (DXA), and physical activity was assessed by an accelerometer. Finally, we analyzed data from the Canadian Community Health Survey (CCHS), which collected data from 9 to 18 year olds (N = 8960), and was representative of Canadian youth. From this survey a single 24-h dietary recall, measured height and weight, sociodemographic, and food insecurity information were available. In both the QUALITY and QCAHS study, >90% of youth had suboptimal vitamin D levels (plasma 25(OH)D < 75 nmol·L–1) at the end of winter and beginning of spring. In the QCAHS study, older youth had a higher prevalence of vitamin D deficiency (25(OH)D < 27.5 nmol·L–1) (>10%) than younger youth, and girls from low-income households had lower plasma 25(OH)D concentrations. In the QUALITY study, milk consumption and physical activity had modest associations with plasma 25(OH)D, corresponding to 2.9 nmol·L–1 and 2.1 nmol·L–1 higher plasma 25(OH)D per standard deviation increase in these exposures, respectively. In the CCHS study, we found evidence that milk intake was being displaced by sweetened beverages among low-income boys and food insecure girls. We conclude that population-wide measures to increase dietary vitamin D intake should be examined in Canadian youth.
Ferrous iron oxidation in moderately thermophilic acidophileSulfobacillus sibiricusN1T
Authors: Dinarieva Tatiana Y. , Zhuravleva Anna E. , Pavlenko Oksana A. , Tsaplina Iraida A. , Netrusov Alexander I. 
Published: October 2010

The iron-oxidizing system of a moderately thermophilic, extremely acidophilic, gram-positive mixotroph, Sulfobacillus sibiricus N1T, was studied by spectroscopic, high-performance liquid chromatography and inhibitory analyses. Hemes B, A, and O were detected in membranes of S. sibiricus N1T. It is proposed that the electron transport chain from Fe2+to O2is terminated by 2 physiological oxidases: aa3-type cytochrome, which dominates in the early-exponential phase of growth, and bo3-type cytochrome, whose role in iron oxidation becomes more prominent upon growth of the culture. Both oxidases were sensitive to cyanide and azide. Cytochrome aa3was more sensitive to cyanide and azide, with Kivalues of 4.1 and 2.5 µmol·L–1, respectively, compared with Kivalues for cytochrome bo3, which were 9.5 µmol·L–1for cyanide and 7.0 µmol·L–1for azide. This is the first evidence for the participation of a bo3-type oxidase in ferrous iron oxidation. The respiratory chain of the mixotroph contains, in addition to the 2 terminal oxidases, a membrane-bound cytochrome b573.
Influence of carbon source on growth and mycotoxin production by isolates of Pyrenophora tritici-repentis from wheat
Authors: Bouras Noureddine , Strelkov Stephen E. 
Published: October 2010

The fungus Pyrenophora tritici-repentis can infect wheat kernels, causing red smudge, and has been shown to produce the anthraquinone mycotoxins emodin, catenarin, and islandicin. The growth of 8 fungal isolates from diverse regions was evaluated on various culture media and was found to be generally slowest on the semisynthetic Fries medium. The choice of carbon source had a significant effect on mycotoxin production, as assessed by high-performance liquid chromatography. The highest emodin concentration (194.18 ± 16.26 µg/g medium) was observed for isolate Alg 3-24 on Fries medium supplemented with fructose, while the highest catenarin concentration (302.54 ±13.92 µg/g medium) was observed for TS93-71B on Fries medium supplemented with starch. Islandicin was not produced by any isolate under the conditions tested. Evaluation of the dynamics of mycotoxin production by isolate 331-2 on V8-potato dextrose agar medium revealed a rapid accumulation of emodin and catenarin during the first week of incubation, followed by a large decline by 14 days. Differences in the growth of and mycotoxin production by isolates of P. tritici-repentis likely resulted from the differential composition of the media and (or) intraspecies variability. Accordingly, the optimization of growth medium should be considered when evaluating the potential of specific isolates for mycotoxin production.
Influence of small doses of various drug vehicles on acetaminophen-induced liver injury
Authors: Kelava Tomislav , Ćavar Ivan , Čulo Filip 
Published: October 2010

The biological effects of drug vehicles are often overlooked, often leading to artifacts in acetaminophen-induced liver injury assessment. Therefore, we decided to investigate the effect of dimethylsulfoxide, dimethylformamide, propylene glycol, ethanol, and Tween 20 on acetaminophen-induced liver injury. C57BL/6 male mice received a particular drug vehicle (0.6 or 0.2 mL/kg, i.p.) 30 min before acetaminophen administration (300 mg/kg, i.p.). Control mice received vehicle alone. Liver injury was assessed by measuring the concentration of alanine aminotransferase in plasma and observing histopathological changes. The level of reduced glutathione (GSH) was assessed by measuring total nonprotein hepatic sulfhydrils. Dimethylsulfoxide and dimethylformamide (at both doses) almost completely abolished acetaminophen toxicity. The higher dose of propylene glycol (0.6 mL/kg) was markedly protective, but the lower dose (0.2 mL/kg) was only slightly protective. These solvents also reduced acetaminophen-induced GSH depletion. Dimethylformamide was protective when given 2 h before or 1 h after acetaminophen administration, but was ineffective if given 2.5 h after acetaminophen. Ethanol at the higher dose (0.6 mL/kg) was partially protective, whereas ethanol at the lower dose (0.2 mL/kg) as well as Tween 20 at any dose had no influence. None of the vehicles (0.6 mL/kg) was hepatotoxic per se, and none of them was protective in a model of liver injury caused by d-galactosamine and lipopolysaccharide.
Paeonol attenuates airway inflammation and hyperresponsiveness in a murine model of ovalbumin-induced asthma
Authors: Du Qiang , Feng Gan-Zhu , Shen Li , Cui Jin , Cai Jian-Kang 
Published: October 2010

Paeonol, the main active component isolated from Moutan Cortex, possesses extensive pharmacological activities such as anti-inflammatory, anti-allergic, and immunoregulatory effects. In the present study, we examined the effects of paeonol on airway inflammation and hyperresponsiveness in a mouse model of allergic asthma. BALB/c mice sensitized and challenged with ovalbumin were administered paeonol intragastrically at a dose of 100 mg/kg daily. Paeonol significantly suppressed ovalbumin-induced airway hyperresponsiveness to acetylcholine chloride. Paeonol administration significantly inhibited the total inflammatory cell and eosinophil count in bronchoalveolar lavage fluid. Treatment with paeonol significantly enhanced IFN-γ levels and decreased interleukin-4 and interleukin-13 levels in bronchoalveolar lavage fluid and total immunoglobulin E levels in serum. Histological examination of lung tissue demonstrated that paeonol significantly attenuated allergen-induced lung eosinophilic inflammation and mucus-producing goblet cells in the airway. These data suggest that paeonol exhibits anti-inflammatory activity in allergic mice and may possess new therapeutic potential for the treatment of allergic bronchial asthma.
Effects of flooding and riparian buffers on survival of muskrats (Ondatra zibethicus) across a flashiness gradient
Authors: Ahlers A. A. , Schooley R. L. , Heske E. J. , Mitchell M. A. 
Published: October 2010

Increased agricultural production within the Grand Prairie region, USA, has resulted in drainage of most natural wetlands within the landscape. Muskrats ( Ondatra zibethicus (L., 1766)) in this region have shifted much of their distribution to riparian habitats that have unstable flow regimes and flood inundation times that could be related to position within watersheds. We investigated predation risk of radio-marked riparian muskrats during flooding events in relation to landscape position. We used known-fate models and an information–theoretic approach to examine effects of age, season, hydrology, and riparian width on weekly survival rates. During flooding events, muskrats positioned farther from headwaters were displaced for longer, as well as exposed to predation from terrestrial predators for longer, than those positioned closer to headwaters. However, this increased exposure during floods did not translate into lower survival because most mortalities were due to predation by American mink ( Neovison vison (Schreber, 1777)) along stream edges during nonflooding periods. Weekly survival of muskrats was lower in winter (mean = 0.9377, SE = 0.1793) than in nonwinter (mean = 0.9770, SE = 0.0116) and was positively related to riparian width. Larger riparian buffers can increase muskrat survival in small streams and agricultural ditches within highly altered, human-dominated agroecosystems. Our study provides a rare example of linking riparian buffers to fitness for a stream-associated organism.
Danger Signaling Protein HMGB1 Induces a Distinct Form of Cell Death Accompanied by Formation of Giant Mitochondria
Authors: Gdynia Georg , Keith Martina , Kopitz Jürgen , Bergmann Marion , Fassl Anne , Weber Alexander N.R. , George Julie , Kees Tim , Zentgraf Hans-Walter , Wiestler Otmar D. , Schirmacher Peter , Roth Wilfried 
Published: October 2010

Abstract
Cells dying by necrosis release the high-mobility group box 1 (HMGB1) protein, which has immunostimulatory effects. However, little is known about the direct actions of extracellular HMGB1 protein on cancer cells. Here, we show that recombinant human HMGB1 (rhHMGB1) exerts strong cytotoxic effects on malignant tumor cells. The rhHMGB1-induced cytotoxicity depends on the presence of mitochondria and leads to fast depletion of mitochondrial DNA, severe damage of the mitochondrial proteome by toxic malondialdehyde adducts, and formation of giant mitochondria. The formation of giant mitochondria is independent of direct nuclear signaling events, because giant mitochondria are also observed in cytoplasts lacking nuclei. Further, the reactive oxygen species scavenger N-acetylcysteine as well as c-Jun NH2-terminal kinase blockade inhibited the cytotoxic effect of rhHMGB1. Importantly, glioblastoma cells, but not normal astrocytes, were highly susceptible to rhHMGB1-induced cell death. Systemic treatment with rhHMGB1 results in significant growth inhibition of xenografted tumors in vivo. In summary, rhHMGB1 induces a distinct form of cell death in cancer cells, which differs from the known forms of apoptosis, autophagy, and senescence, possibly representing an important novel mechanism of specialized necrosis. Further, our findings suggest that rhHMGB1 may offer therapeutic applications in treatment of patients with malignant brain tumors. Cancer Res; 70(21); 8558–68. ©2010 AACR.
Zoledronic Acid Reduces Bone Loss and Tumor Growth in an Orthotopic Xenograft Model of Osteolytic Oral Squamous Cell Carcinoma
Authors: Martin Chelsea K. , Werbeck Jillian L. , Thudi Nanda K. , Lanigan Lisa G. , Wolfe Tobie D. , Toribio Ramiro E. , Rosol Thomas J. 
Published: October 2010

Abstract
Squamous cell carcinoma (SCC) is the most common form of oral cancer. Destruction and invasion of mandibular and maxillary bone frequently occurs and contributes to morbidity and mortality. We hypothesized that the bisphosphonate drug zoledronic acid (ZOL) would inhibit tumor-induced osteolysis and reduce tumor growth and invasion in a murine xenograft model of bone-invasive oral SCC (OSCC) derived from an osteolytic feline OSCC. Luciferase-expressing OSCC cells (SCCF2Luc) were injected into the perimaxillary subgingiva of nude mice, which were then treated with 100 μg/kg ZOL or vehicle. ZOL treatment reduced tumor growth and prevented loss of bone volume and surface area but had no effect on tumor invasion. Effects on bone were associated with reduced osteolysis and increased periosteal new bone formation. ZOL-mediated inhibition of tumor-induced osteolysis was characterized by reduced numbers of tartrate-resistant acid phosphatase–positive osteoclasts at the tumor-bone interface, where it was associated with osteoclast vacuolar degeneration. The ratio of eroded to total bone surface was not affected by treatment, arguing that ZOL-mediated inhibition of osteolysis was independent of effects on osteoclast activation or initiation of bone resorption. In summary, our results establish that ZOL can reduce OSCC-induced osteolysis and may be valuable as an adjuvant therapy in OSCC to preserve mandibular and maxillary bone volume and function. Cancer Res; 70(21); 8607–16. ©2010 AACR.
Hierarchical Clustering of Human Papilloma Virus Genotype Patterns in the ASCUS-LSIL Triage Study
Authors: Wentzensen Nicolas , Wilson Lauren E. , Wheeler Cosette M. , Carreon Joseph D. , Gravitt Patti E. , Schiffman Mark , Castle Philip E. 
Published: October 2010

Abstract
Anogenital cancers are associated with ∼13 carcinogenic human papilloma virus (HPV) types in a broader group that cause cervical intraepithelial neoplasia (CIN). Multiple concurrent cervical HPV infections are common, which complicates the attribution of HPV types to different grades of CIN. Here we report the analysis of HPV genotype patterns in the atypical squamous cells of undetermined significance–low-grade squamous intraepithelial lesion triage study with the use of unsupervised hierarchical clustering. Women who underwent colposcopy at baseline (n = 2,780) were grouped into 20 disease categories based on histology and cytology. Disease groups and HPV genotypes were clustered with the use of complete linkage. Risk of 2-year cumulative CIN3+, viral load, colposcopic impression, and age were compared between disease groups and major clusters. Hierarchical clustering yielded four major disease clusters: cluster 1 included all CIN3 histology with abnormal cytology; cluster 2 included CIN3 histology with normal cytology and combinations with either CIN2 or high-grade squamous intraepithelial lesion cytology; cluster 3 included older women with normal or low-grade histology/cytology and low viral load; and cluster 4 included younger women with low-grade histology/cytology, multiple infections, and the highest viral load. Three major groups of HPV genotypes were identified: group 1 included only HPV16; group 2 included nine carcinogenic types, plus noncarcinogenic HPV53 and HPV66; and group 3 included noncarcinogenic types, plus carcinogenic HPV33 and HPV45. Clustering results suggested that colposcopy missed a prevalent precancer in many women with no biopsy/normal histology and high-grade squamous intraepithelial lesion. This result was confirmed by an elevated 2-year risk of CIN3+ in these groups. Our novel approach to study multiple genotype infections in cervical disease with the use of unsupervised hierarchical clustering can address complex genotype distributions on a population level. Cancer Res; 70(21); 8578–86. ©2010 AACR.