bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional involvement of Daxx in gp130‐mediated cell growth and survival in BaF3 cells
Authors: Muromoto Ryuta , Kuroda Makoto , Togi Sumihito , Sekine Yuichi , Nanbo Asuka , Shimoda Kazuya , Oritani Kenji , Matsuda Tadashi 
Published: September 2010

AbstractDeath domain‐associated protein (Daxx) is a multifunctional protein that modulates both cell death and transcription. Several recent studies have indicated that Daxx is a mediator of lymphocyte death and/or growth suppression, although the detailed mechanism is unclear. Previously, we reported that Daxx suppresses IL‐6 family cytokine‐induced gene expression by interacting with STAT3. STAT3 is important for the growth and survival of lymphocytes; therefore, we here examined the role of Daxx in the gp130/STAT3‐dependent cell growth/survival signals. We found that Daxx suppresses the gp130/STAT3‐dependent cell growth and that Daxx endogenously interacts with STAT3 and inhibits the DNA‐binding activity of STAT3. Moreover, small‐interfering RNA‐mediated knockdown of Daxx enhanced the expression of STAT3‐target genes and accelerated the STAT3‐mediated cell cycle progression. In addition, knockdown of Daxx‐attenuated lactate dehydrogenase leakage from cells, indicating that Daxx positively regulates cell death during gp130/STAT3‐mediated cell proliferation. Notably, Daxx specifically suppressed the levels of Bcl2 mRNA and protein, even in cytokine‐unstimulated cells, indicating that Daxx regulates Bcl2 expression independently of activated STAT3. These results suggest that Daxx suppresses gp130‐mediated cell growth and survival by two independent mechanisms: inhibition of STAT3‐induced transcription and down‐regulation of Bcl2 expression.
Lck regulates IL‐10 expression in memory‐like Th1 cells
Authors: Kemp Kyeorda L. , Levin Steven D. , Stein Paul L. 
Published: September 2010

AbstractThe Src family kinase Lck is thought to facilitate Th2 differentiation; however, its role in Th1 cells has not been well explored. Using mice that lack Lck in mature T cells, we find that lck−/− Th1 skewed cells have normal expression of T‐bet and produce IFN‐γ at WT levels. However, there is a 3‐fold increase in IL‐10 producing cells in the mutant cultures. These cells do not have elevated levels of IL‐4, GATA3, IL‐17 or Foxp3, indicating that they are not Th2, Th17, or Foxp3+ T regulatory cells (Treg). Nor do these cells behave in a similar manner as the type 1 Treg. Most of the IL‐10 in the lck−/− Th1 cultures is derived from the memory/activated subset, as the cytokine profile from Th1 cultures established from purified CD62L+ (naïve) cells are similar to WT cells. Furthermore, this IL‐10 expression appears to be dependent on IL‐12 and correlates with elevated c‐Maf. These data highlight a previously unappreciated role for Lck in regulating IL‐10 in Th1 cells.
Myeloid‐derived suppressor cells in parasitic infections
Authors: Van Ginderachter Jo A. , Beschin Alain , De Baetselier Patrick , Raes Geert 
Published: September 2010

AbstractMyeloid‐derived suppressor cells (MDSC) are a heterogeneous population of immature myeloid cells that share a common property of suppressing immune responses. Several helminth and protozoan parasite species have developed efficient strategies to increase the rate of medullary or extramedullary myelopoiesis and to induce the expansion and accumulation of immature myeloid cells such as MDSC. In this review, we examine current knowledge on the factors mediating enhanced myelopoiesis and MDSC induction and recruitment during parasitic infections and how the MDSC phenotype and mechanism of immune modulation and suppression depends on the factors they encounter within the host. Finally, we place MDSC expansion in the context of the critical balance between parasite elimination and pathogenicity to the host and suggest attractive avenues for future research.
At last — rats lacking B cells
Authors: Holmdahl Rikard 
Published: September 2010

AbstractThe rat model is a widely used animal model in research, its popularity perhaps only surpassed by the mouse model. Rats are the preferred models for the study of transplantation and certain tumor and autoimmune diseases. In particular, the understanding of cardiovascular‐related diseases and chronic inflammation has depended widely on the rat, from which models for both multiple sclerosis and rheumatoid arthritis have been derived. Until now, research in the rat has been hampered by a lack of precise gene‐targeting technology in this model. This limitation, however, is rapidly changing; a recent example is the availability of B‐cell‐deficient rat strains obtained by the newly established zinc finger nucleases‐targeting technology. As described in this issue of the European Journal of Immunology, genetic targeting of the rat, for example, using zinc finger nucleases‐targeting technology, is likely to rapidly drive progress in the understanding of not only B‐cell biology but also in the general understanding of rat disease models.
Observers' benefit finding for victims: Consequences for perceived moral obligations
Authors: Warner Ruth H. , Branscombe Nyla R. 
Published: September 2010

AbstractIn three studies we examined how observers making meaning of victimization by finding benefits for the victim leads to the perception that victims are morally obligated to help others and not do harm. In Experiment 1, participants perceived a victim as having greater moral obligations when the meaning of victimization was considered for the victim rather than the perpetrator. This effect on moral obligations was mediated by the extent to which participants believed victims should have found benefits. Experiments 2 and 3 examined the consequences when victims fail or fulfill their moral obligations. Greater social distance from a victim who did harm was sought when participants focused on the meaning of victimization for the victim as compared to the perpetrator. Less social distance from a victim who helped was sought when participants focused on the meaning of victimization for the victim as compared to the perpetrator or when they made no meaning. These studies show that how observers make meaning of victimization has implications for subsequent responses to victims. Copyright © 2010 John Wiley & Sons, Ltd.
Alpha‐1 antitrypsin variants in plasma from HIV‐infected patients revealed by proteomic and glycoproteomic analysis
Authors: Zhang Lijun , Jia Xiaofang , Zhang Xiaojun , Cao Jing , Yang Pengyuan , Qiu Chao , Shen Yinzhong , Ma Fang , Liu Li , Sun Jianjun , Shen Fang , Yin Lin , Liu Liwen , Yao Yamin , Lu Hongzhou 
Published: September 2010

AbstractNovel tools are necessary to explore proteins related to human immunodeficiency virus (HIV) infection. In this work, proteomic and glycoproteomic technology were employed to examine plasma samples from HIV‐positive patients. Through comparative proteome analysis of normal and HIV‐positive plasma samples, 19 differentially expressed protein spots related to 12 non‐redundant proteins were identified by ESI‐ion trap MS. Among these, the 130‐kDa isoform of α‐1‐antitrypsin was found to be decreased in HIV‐positive patients while another variant with a molecular weight of 40 kDa was increased. SWISS‐2‐D‐PAGE reference gel and protein sequence comparisons of the 40‐kDa protein showed homology with α‐1‐antitrypsin minus the N‐terminus, and its identity was further confirmed by 1‐D Western blotting and glycoproteomic analysis. In all, our results showed that proteomics and glycoproteomics are powerful tools for discovering proteins related to HIV infection. Furthermore, this 40‐kDa variant of α‐1‐antitrypsin found in the plasma of HIV‐positive individuals may prove to be a potentially useful biomarker for anti‐HIV research according to bioinformatics analysis.
In situ cyclic tests on existing stone masonry walls and strengthening solutions
Authors: Costa Alexandre A. , Arêde António , Costa Aníbal , Oliveira Carlos Sousa 
Published: September 2010

AbstractThe present work reports on an in situ experimental test campaign carried out on abandoned traditional masonry houses after the 9th July 1998 earthquake that seriously hit the Faial island of Azores. For the testing purposes, an experimental test setup was developed based on a self‐equilibrated scheme, which is herein described reporting on the advantages and drawbacks of this in situ test setup.Five specimens were tested aiming at characterizing the out‐of‐plane behavior of stone masonry walls and strengthening solutions recommended for post‐earthquake interventions. A detailed comparison between solutions' efficiency is presented including a cost vs benefit analysis.In order to assess the efficiency of the developed test setup for other applications on stone masonry walls, an in‐plane test on an existing URM panel is also presented. Several related issues are discussed, namely the advantages of dealing with the real boundary conditions and the capacity of providing valuable information of the response, as well as a detailed analysis of the obtained results.The authors believe that this work provides an increase in knowledge on the seismic behavior of the existing masonry constructions, resulting from the development of an in situ test setup and the efficiency quantification of strengthening solutions. Therefore, the work is thought to positively contribute for the preservation of architectural heritage and for its seismic vulnerability reduction. Copyright © 2010 John Wiley & Sons, Ltd.
Enhanced cognitive behavioural therapy for an adolescent with purging disorder: A case report
Authors: Sysko Robyn , Hildebrandt Tom 
Published: September 2010

AbstractObjectiveTo present a case report detailing the use of an enhanced form of cognitive behaviour therapy (CBT). The treatment was provided to an adolescent with an eating disorder not otherwise specified (EDNOS) diagnosis, and included a focus on the additional maintaining mechanisms of mood intolerance and interpersonal problems.CaseThis case began as an unsuccessful attempt at family therapy, where the underlying dysfunction exacerbated symptoms and demoralized the family. The therapist subsequently chose to utilize an enhanced version of CBT to simultaneously address the patient's symptoms and try to effect change across multiple domains. A description of the patient's eating disorder pathology, the 29‐session treatment, and outcome, are provided.ConclusionThis case study illustrates that it is possible to successfully use enhanced CBT with developmentally appropriate adaptations in the treatment of a young patient with an EDNOS diagnosis, as suggested by Cooper and Stewart (2008). Copyright © 2010 John Wiley & Sons, Ltd and Eating Disorders Association.
Tectonic uplift, sea level changes and Plio‐Pleistocene evolution of a coastal karst system: the Mount Saint Paul (Palawan, Philippines)
Authors: Piccini L. , Iandelli N. 
Published: September 2010

AbstractThe St. Paul karst (Palawan, Philippines) is a tropical coastal karst, consisting of towers, cones, huge depressions and large caves. This area hosts the Puerto Princesa Subterranean River (PPSR, 24 km long), whose main entrance is a large spring along the coast and which is one of the largest cave complexes in eastern Asia.A geomorphological study performed by several field surveys and a morphometric analysis of the digital terrain model (DTM) and 3D cave models, allowed formulation of a first evolutionary framework of the karst system. The DTM was extracted from maps and aerial photographs in order to find different generations of ‘relict’ landforms, through the morphometric analysis of topographic surface and karst landforms.Several features suggest a long and multi‐stage evolution of the karst, whose age ranges from Pliocene to present. The southern and northern sectors of the area differ in their altimetric distribution of caves. In the southern sector, some large caves lie between 300 and 400 m asl and were part of an ancient system that developed at the base level of a past river network. In the northern sector, some mainly vadose caves occur, with a phreatic level at 120–130 m asl. An important phase of base‐level cave development is well documented in the inactive passages of PPSR at 50–80 m asl. Morphological features, such as horizontal solution passages and terraced deposits, suggest a phase of stillstand of the base level, which is recorded in the topography as low‐relief surfaces at 40–50 m asl. The age of this phase is probably Early Pleistocene, on the basis of assumed uplift rates. The more recent caves are still active, being located at the current sea level, but they show more than one cycle of flooding and dewatering (with calcite deposition). In the PPSR, several morphologic features, such as two main water level notches at +12·4 and +7·7 m asl and terraced alluvial deposits, suggest that the lower and active level passed through more than two high‐stands of sea level and so it could have formed throughout most of the Middle‐Late Pleistocene. Copyright © 2010 John Wiley & Sons, Ltd.
Synthesis of fragrant heterocyclic thioketo analogues of jasmone under microwave conditions
Authors: Pawełczyk Anna , Zaprutko Lucjusz 
Published: September 2010

AbstractRapid, solvent‐free and cleaner conversions of fragrant heteroketones, heterocyclic analogues of natural jasmone, to the corresponding thioanalogues were described. Obtained structures of thioketohetero derivatives are based on pyrrolidinone, oxazolidinone and thiazolidinone systems with saturated and 2‐double or 2‐triple unsaturated five‐carbon side chain. Thionations were performed by the use of Lawesson's reagent without any solvent or solid support under microwave conditions with good yield. Odour evaluation and relationships between their chemical structure and osmic properties for all synthesized fragrant compounds have been studied. It has been shown that the majority of them have exhibited interesting, very intensive, durable and fixative fragrant properties. Copyright © 2010 John Wiley & Sons, Ltd.
Ionic Liquids and Their Hosting by Polymers for HT‐PEMFC Membranes
Authors: Iojoiu C. , Hana M. , Molmeret Y. , Martinez M. , Cointeaux L. , El Kissi N. , Teles J. , Leprêtre J.‐C. , Judeinstein P. , Sanchez J.‐Y. 
Published: September 2010

AbstractThe paper deals with proton‐conducting ionic liquids (PCILs) for use, in combination with functional polymers, in membranes operating in high temperature PEMFC. Monoammoniums derived from monoamines and half‐neutralised diamines were investigated in the form of triflates. Promising results were obtained with the half‐neutralised diamine‐based PCIL, its conduction being governed by both Grotthuss‐like and vehicular mechanisms, the respective contributions of which depend on temperature. In addition, their blending with Nafion results in a distinct reinforcement of the membrane.
Effects of immersion time and frequency of water exchange on durability of etch‐and‐rinse adhesive
Authors: Skovron Leandro , Kogeo Diogo , Gordillo Luiz Alfonso Arana , Meier Marcia M. , Gomes Osnara M. M. , Reis Alessandra , Loguercio Alessandro D. 
Published: September 2010

AbstractThis study evaluated the immediate and long‐term bond strength to dentin (microtensile bond strength, μTBS) and silver nitrate uptake (SNU) of a three‐ and two‐step etch‐and‐rinse adhesive under different water immersion times and frequency of water exchange. The adhesives and composite resin were applied according to the manufacturer's instructions in a flat occlusal demineralized dentin of 48 molars. Teeth were assigned to four groups of immersion time (immediate and 1, 3, and 6 months), sectioned to obtain resin–dentin beams, and then subdivided into three groups of water exchange (daily, weekly, and monthly) before being tested in tension. Two resin–dentin beams from each tooth were immersed in silver nitrate and analyzed by SEM. Significant difference in μTBS and SNU was detected for both adhesives (p ≤ 0.0001 for the cross‐product interaction). For Adper Single Bond 2, the most pronounced reductions of μTBS were observed for the daily exchange groups in all times. For Adper Scotchbond Multi Purpose, 1‐month immersion period was not capable to induce degradation of the dentin bonds, except when the water was exchanged daily. For both adhesives, all storage regimens showed increased SNU results only after 6‐month water storage; this being more pronounced for daily water exchange groups. For both adhesives, the highest SNU was observed in the daily water exchange group. The daily water exchange is a fast and reliable in vitro aging method for testing the durability of the adhesive interface produced by adhesive systems. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010.
Bioactive bone cement with a low content of titania particles without postsilanization: Effect of filler content on osteoconductivity, mechanical properties, and handling characteristics
Authors: Fukuda C. , Goto K. , Imamura M. , Nakamura T. 
Published: September 2010

AbstractIn this study, we developed three types of polymethylmethacrylate (PMMA)‐based composite cement with low contents of nonsilanized titania particles (5, 10, and 20 wt % TiO2, respectively: designated T5, T10, and T20). The osteoconductivity, mechanical properties, and handling characteristics of these cements were compared with those of commercially available PMMA cement (PMMAc). The cement was inserted into rat tibiae and solidified in situ. After 6 and 12 weeks, tibiae were removed for evaluation of osteoconductivity using Stevenel's Blue and Van Gieson's picrofuchsin staining. The affinity indices reflecting the osteoconductivity of T10 and T20 were 33.4 ± 12.8 and 56.5 ± 14.1 at 6 weeks, and 67.0 ± 18.0 and 65.0 ± 51.7 at 12 weeks, respectively, and were significantly higher than for PMMAc (p < 0.01). The compressive and flexural strengths of T5, T10, and T20 exceeded those of PMMAc, whereas the elasticity did not differ significantly. Scanning electron microscopy and energy‐dispersive X‐ray microanalysis showed that the micron‐sized and spherical titania particles were well dispersed in T20 and were exposed on the surface of the cement that made direct contact with bone. These results show that T20 is a promising bioactive bone cement for use in prosthesis fixation. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010.
Simultaneous determination of proline and pipemidic acid in human urine by capillary electrophoresis with electrochemiluminescence detection
Authors: Sun Hanwen , Li Liqing , Su Ming 
Published: September 2010

AbstractPipemidic acid is extensively used in the treatment of Gram‐negative urinary tract infections, and the contents of proline in human urine vary in association with chronic uremia. The simultaneous determination of pipemidic acid and proline in human urine is of significance for quality control of the dosage and clinical study. The coupling of Ru(bpy)‐based electrochemiluminescence detection with capillary electrophoresis was developed for the simultaneous determination of proline and pipemidic acid in human urine. Parameters related to the separation and detection were investigated and optimized. The standard curves were linear between 0.1 and 90 µg mL−1 for proline and between 0.4 and 100 µg mL−1 for pipemidic acid. Underop‐timized conditions, the detection limits (3σ) were 0.02 µg mL−1 for proline and 0.06 µg mL−1 for pipemidic acid. Relative standard derivations for the electrochemiluminescence intensity and the migration time were 3.2 and 0.9% for proline and 3.7 and 1.2% for pipemidic acid, respectively. The developed method was successfully applied to determine proline and pipemidic acid in human urine. The result showed that the content and decreasing rates of proline in urine for male were higher than that for female, and the content and decreasing rate of pipemidic acid in urine for male and female were consistent, respectively. J. Clin. Lab. Anal. 24:327–333, 2010. © 2010 Wiley‐Liss, Inc.
Re‐evaluation of cord blood arterial and venous reference ranges for pH, pO2, pCO2, according to spontaneous or cesarean delivery
Authors: Kotaska K. , Urinovska R. , Klapkova E. , Prusa R. , Rob L. , Binder T. 
Published: September 2010

AbstractUmbilical cord blood gas analysis (pO2 and pCO2) is now recommended in all high‐risk baby deliveries and in some centers it is performed routinely following all deliveries. The aim of this study was to re‐evaluate cord blood arterial and venous reference ranges for pH, pO2, pCO2 in newborns, delivered by spontaneous vaginal delivery (SVD) and by cesarean section (CS) performed in Faculty Hospital Motol. Two groups of subjects were selected for the study. Group I consisted of 303 newborns with SVD. Group II consisted of 189 newborns delivered by cesarean section. Cord blood samples were analyzed for standard blood gas and pH, using the analytical device Rapid Lab 845 and Rapid Lab 865. We obtained reference values expressed as range (lower and upper reference value expressed as 2.5 and 97.5 percentiles) for cord blood in newborns with SVD: arterial cord blood: pH=7.01–7.39; pCO2=4.12–11.45 kPa; pO2=1.49–5.06 kPa; venous cord blood: pH=7.06–7.44; pCO2=3.33–9.85 kPa; pO2=1.80–6.29 kPa. We also obtained reference values for cord blood in newborns delivered by CS: arterial cord blood: pH=7.05–7.39; pCO2=5.01–10.60 kPa; pO2=1.17–5.94 kPa; venous cord blood: pH=7.10–7.42; pCO2=3.88–9.36 kPa; pO2=1.98–7.23 kPa. Re‐evaluated reference ranges play essential role in monitoring conditions of newborns with spontaneous and caesarean delivery. J. Clin. Lab. Anal. 24:300–304, 2010. © 2010 Wiley‐Liss, Inc.
Benefits of blood group genotyping in multi‐transfused patients from the south of Brazil
Authors: Guelsin Gláucia Andréia Soares , Sell Ana Maria , Castilho Lilian , Masaki Viviane Lika , Melo Fabiano Cavalcante , Hashimoto Margareth Naomi , Higa Tatiana Takahashi , Hirle Loide Souza , Visentainer Jeane Eliete Laguila 
Published: September 2010

AbstractWe evaluated the usefulness of blood group genotyping as a supplement to hemagglutination to determine the red blood cell (RBC) antigen profile of polytransfused patients with hematological diseases and renal failure. Seventy‐nine patients were selected. They all received more than three units of blood and eight (10%) had already clinical significant alloantibodies occurring alone or in combination against Rh, K, Fya, and Di antigens. DNA was prepared from blood samples and RHCE*E/e, KEL*01/KEL*02, FY*01/FY*02 and JK*01/JK*02 alleles were determined by using PCR‐RFLP. RHD*/RHD*Ψ and RHCE*C/c were tested using multiplex PCR. Discrepancies for Rh, Kell, Duffy, and Kidd systems were found between the phenotype and genotype‐derived phenotype in 16 of the 38 chronically transfused patients. The genotypes of these patients were confirmed by DNA array analysis (HEA Beadchip™; Bioarray Solutions, Warren, NJ). Genotyping was very important for the determination of the true blood groups of the polytransfused patients, helped in the identification of suspected alloantibodies and in the selection of antigen‐negative RBCs for transfusion. J. Clin. Lab. Anal. 24:311–316, 2010. © 2010 Wiley‐Liss, Inc.