bluuebunny/crossref_metadata_2025_split huggingface dataset:
High-affinity detection of endogenously biotinylated neuroligin-1 at excitatory and inhibitory synapses using a tagged knock-in mouse strain
Authors: Ducrot Charles , Drouet Adèle , Tessier Béatrice , Desquines Chloé , Mazzouzi Rania-Cérine , Favereaux Alexandre , Letellier Mathieu , Thoumine Olivier 
Published: June 2024

AbstractNeuroligins (NLGNs) are important cell adhesion molecules mediating trans-synaptic contacts between neurons. However, the high-yield biochemical isolation and visualization of endogenous NLGNs have been hampered by the lack of efficient antibodies to these proteins. Thus, to reveal their sub-cellular distribution, binding partners, and synaptic function, NLGNs have been extensively manipulated using knock-down, knock-out, or over-expression approaches, overall leading to controversial results. As an alternative to the manipulation of NLGN expression level, we describe here the generation of a new transgenic mouse strain in which native NLGN1 was N-terminally tagged with a small biotin acceptor peptide (bAP) that can be enzymatically biotinylated by the exogenous delivery of biotin ligase. After showing that knock-in mice exhibit normal behavior as well as similar synaptic number, ultrastructure, transmission properties, and protein expression levels when compared to wild type counterparts, we exploited the fact that biotinylated bAP-NLGN1 can be selectively isolated or visualized using high-affinity streptavidin conjugates. Using immunoblotting and immunofluorescence, we show that bAP-NLGN1 binds both PSD-95 and gephyrin and distributes equally well at excitatory and inhibitory synapses, challenging the historical view that NLGN1 is exclusively localized at excitatory synapses. Using super-resolution fluorescence microscopy and electron microscopy, we further highlight that bAP-NLGN1 forms in the synaptic cleft a subset of nanodomains each containing a few NLGN1 dimers, while the number of nanodomains per synapse positively scales with the post-synapse size. Overall, our study not only provides a novel, extensively characterized transgenic mouse model which will be made available to the scientific community, but also an unprecedented view of the nanoscale organization of endogenous NLGN1.
Mutant HTT protein decreases with CAG repeat expansion: implications for therapeutics and bioassays
Authors: Landles Christian , Osborne Georgina F. , Phillips Jemima , Pico Maria Canibano , Nita Iulia M. , Ali Nadira , Greene Jonathan R. , Sathasivam Kirupa , Bates Gillian P. 
Published: June 2024

ABSTRACTHuntington’s disease is an inherited neurodegenerative disorder caused by a CAG repeat expansion that encodes a polyglutamine tract in the HTT protein. The mutant CAG repeat is unstable and expands in specific brain cells and peripheral tissues throughout life. Genes involved in the DNA mismatch repair pathways, known to act on expansion, have been identified as genetics modifiers, therefore, it is the rate of somatic CAG repeat expansion that drives the age of onset and rate of disease progression. In the context of an expanded CAG repeat, theHTTpre-mRNA can be alternatively processed to generate theHTT1atranscript, that encodes the aggregation prone and highly pathogenic HTT1a protein. This may be a mechanism through which somatic CAG repeat expansion exerts its pathogenic effects, as the longer the CAG repeat, the moreHTT1aand HTT1a is produced.The allelic series of knock-in mouse models:HdhQ20,HdhQ50,HdhQ80,HdhQ111, CAG140 and zQ175 with polyQ expansions of 20, 50, 80, 111 140 and ∼190 can be used to model the molecular and cellular consequences of CAG repeat expansion within a single neuron. By western blot of cortical lysates, we found that mutant HTT levels decreased with increasing CAG repeat length; mutant HTT was only 23% and 10% of wild-type levels in CAG140 and zQ175 cortices, respectively. To identify the optimal bioassays for detecting the full-length HTT and HTT1a isoforms, we interrogated the pairwise combinations of seven well-characterized antibodies on both the HTRF and MSD platforms. In total we tested 32 HTRF and 32 MSD assays to detect ‘full-length mutant HTT’, HTT1a, ‘total mutant HTT’ (full-length HTT and HTT1a) and ‘total full-length HTT’ (mutant and wild type). None of these assays recapitulated the full-length mutant HTT levels as measured by western blot. We recommend using isoform- and species-specific assays that detect either full-length mutant HTT, HTT1a or wild-type HTT as opposed to those that detect more than one isoform simultaneously.Our finding that as the CAG repeat expands, full-length mutant HTT levels decrease, whilstHTT1aand HTT1a levels increase has implications for therapeutic strategies. If mutant HTT levels in cells containing (CAG)200are only 10% of wild-type, HTT-lowering strategies targeting full-lengthHTTat sequences 3’ to intron 1HTTwill predominantly lower wild-type HTT, as mutant HTT levels in these cells are already depleted. These data support a therapeutic strategy that lowersHTT1aand depletes levels of the HTT1a protein.
Neural crest and periderm-specific requirements ofIrf6during neural tube and craniofacial development
Authors: Carroll Shannon H. , Schafer Sogand , Dalessandro Eileen , Ho Thach-Vu , Chai Yang , Liao Eric C. 
Published: June 2024

AbstractIRF6is a key genetic determinant of syndromic and non-syndromic cleft lip and palate. The ability to interrogate post-embryonic requirements ofIrf6has been hindered, as globalIrf6ablation in the mouse causes neonatal lethality. Prior work analyzingIrf6in mouse models defined its role in the embryonic surface epithelium and periderm where it is required to regulate cell proliferation and differentiation. Several reports have also describedIrf6gene expression in other cell types, such as muscle, and neuroectoderm. However, analysis of a functional role in non-epithelial cell lineages has been incomplete due to the severity and lethality of theIrf6knockout model and the paucity of work with a conditionalIrf6allele. Here we describe the generation and characterization of a newIrf6floxed mouse model and analysis ofIrf6ablation in periderm and neural crest lineages. This work found that loss ofIrf6in periderm recapitulates a mildIrf6null phenotype, suggesting thatIrf6-mediated signaling in periderm plays a crucial role in regulating embryonic development. Further, conditional ablation ofIrf6in neural crest cells resulted in an anterior neural tube defect of variable penetrance. The generation of this conditionalIrf6allele allows for new insights into craniofacial development and new exploration into the post-natal role ofIrf6.
Testing axionic dark matter during gravitational reheating
Authors: Barman Basabendu , Datta Arghyajit 
Published: June 2024

Assuming axions are potential dark matter (DM) candidate that make up all of the DM abundance, we discuss production of axions via (i) standard misalignment mechanism during the period of reheating and (ii) graviton-mediated 2-to-2 scattering of the inflaton and bath particles, where the inflaton ϕ oscillates in a monomial potential V(ϕ)∝ϕk with a general equation of state. Considering reheating takes place purely gravitationally, mediated by massless gravitons, we explore the viable region of the parameter space that agrees with the observed DM relic abundance, satisfying bounds from big bang nucleosynthesis and cosmic microwave background radiation. We also discuss complementarity between dedicated axion search experiments and futuristic gravitational wave search facilities in probing the viable parameter space, thanks to the presence of detectable primordial gravitational waves with an inflationary origin.

Published by the American Physical Society
2024
Gluon moment and parton distribution function of the pion from
Nf=2+1+1
lattice QCD
Authors: Good William , Hasan Kinza , Chevis Allison , Lin Huey-Wen 
Published: June 2024

We present the first calculation of the pion gluon moment from lattice QCD in the continuum-physical limit. The calculation is done using clover fermions for the valence action with three pion masses, 220, 310 and 690 MeV, and three lattice spacings, 0.09, 0.12, and 0.15 fm, using ensembles generated by MILC Collaboration with 2+1+1 flavors of highly improved staggered quarks (HISQ). On the lattice, we nonperturbatively renormalize the gluon operator in RI/MOM scheme using the cluster-decomposition error reduction (CDER) technique to enhance the signal-to-noise ratio of the renormalization constant. We extrapolate the pion gluon moment to the continuum-physical limit and obtain ⟨x⟩g=0.394(58)stat+NPR(39)mixing in the MS¯ scheme at 2 GeV, with first error being the statistical error and uncertainties in nonperturbative renormalization, and the second being a systematic uncertainty estimating the effect of ignoring quark mixing. Our pion gluon momentum fraction has a central value lower than two recent single-ensemble lattice-QCD results near physical pion mass but is consistent with the recent global fits by JAM and xFitter and with most QCD-model estimates.

Published by the American Physical Society
2024
Investigating the development of narratives in directors' reports: an analysis of Barilla over six decades
Authors: Gianfelici Cristina , Martin-Sardesai Ann , Guthrie James 
Published: June 2024

PurposeThis research explores how contextual elements and significant events influence the changing storylines within a company's directors' reports spanning a period of six decades. These elements and events encompass the internal dynamics of the family that owns the company, industry-specific advancements, political and socioeconomic climates, and explicit guidelines related to corporate reporting.Design/methodology/approachThis research employs a case study methodology to analyse the directors' reports of Barilla, a prominent Italian food manufacturer, within the theoretical framework of historical institutionalism. A systematic content analysis is conducted on sixty directors' reports published between 1961 and 2021. The study also identifies and examines significant contextual events within this six-decade period, which are linked to four key institutional factors.FindingsBased on the research findings, the directors' reports exhibited notable fluctuations throughout the studied timeframe in reaction to shifts in the institutional setting. Our investigation highlights that each institutional element experienced crucial pivotal moments, and given their interconnected nature, modifications in one factor impacted the others. It was noted that these pivotal moments resulted in alterations in the directors' reports' content across various thematic areas. Additionally, despite Barilla being a multinational company, it was found that national events within Italy had a more pronounced influence on the evolving narratives than global events.Originality/valuePrevious research on directors' reports or chairman's statements has primarily focused on the influence of macro-level institutional factors on the narratives. In contrast, our study considers both macro-level and micro-level institutions, specifically examining the internal events within a family business and how they shape the content of directors' reports. Our study is also distinctive in its analysis of specific critical junctures and their interactions with the investigated institutional factors. Additionally, to the best of our knowledge, few existing studies span a timeframe of sixty years, particularly concerning an Italian company.
Does ownership structure affect carbon emission disclosure?
Authors: Bedi Ankita , Singh Balwinder 
Published: June 2024

PurposeThe current research strives to shed light on how ownership structure can impact carbon emission disclosure.Design/methodology/approachThe present study is based on S&P BSE 500 Indian firms. Using manual content analysis, carbon emission disclosure data were collected from a final sample of 318 nonfinancial Indian firms over seven years, i.e. from 2016–17 to 2022–23, having 2,226 firm-year observations. The panel regression has been employed to examine the association between ownership structure and carbon emissions disclosure.FindingsThe results of the study suggest that ownership structure variables, such as institutional and foreign ownership, exert a positive and significant influence on carbon emission disclosure. Conversely, block-holder ownership is negatively associated with carbon emission disclosure.Practical implicationsThis study enriches the emerging literature on environmental disclosure, climate change, carbon emission disclosure and ownership structure.Social implicationsThe present research work provides treasured acumens to corporate managers, investors, regulators and policymakers as the study corroborates that ownership structure has an imperative role in firms' carbon emission disclosure.Originality/valueExisting literature has determined the impact of ownership structure on environmental disclosure. In contrast, the current research extends the climate change literature by providing novel insights into how ownership structure can influence firms’ carbon emission disclosure. Moreover, to the best of the authors’ knowledge, the present study is the first to scrutinize the relationship between ownership structure and carbon emission disclosure in the Indian context.
Non-linear impedance boundary condition from linear piecewise B-H curve applied to induction heating systems
Authors: Ortega José , Lahuerta Óscar , Carretero Claudio , Martínez Juan Pablo , Acero Jesús 
Published: June 2024

Purpose
This paper aims to apply the non-linear impedance boundary condition (IBC) for a linear piecewise B–H curve in frequency domain simulations to find the equivalent impedance of a simple induction heating system model.

Design/methodology/approach
An electromagnetic description of the inductor system is performed to substitute the effects of the induction load, for a mathematical condition, the so-called IBC. This is suitable to be used in electromagnetic systems involving high conductive materials at medium frequencies, as it occurs in an induction heating system.

Findings
A reduction of the computational cost of electromagnetic simulation through the application of the IBC. The model based on linear piecewise B–H curve simplifies the electromagnetic description, and it can facilitate the identification of the induction load characteristics from experimental measurements.

Practical implications
This work is performed to assess the feasibility of using the non-linear boundary impedance condition of materials with linear piecewise B–H curve to simulate in the frequency domain with a reduced computational cost compared to time domain simulations.

Originality/value
In this paper, the use of the non-linear boundary impedance condition to describe materials with B–H curve by segments, which can approximate any dependence without hysteresis, has been studied. The results are compared with computationally more expensive time domain simulations.
Microfoundations of dynamic capabilities for digital transformation
Authors: Kowalski Mauro , Bernardes Roberto Carlos , Gomes Leonardo , Borini Felipe Mendes 
Published: June 2024

PurposeScholars increasingly focus on digital transformation as a key strategy for incumbent firms to gain competitive advantages. Meeting digital transformation commitments presents challenges, requiring the application and the reconfiguration of dynamic capabilities. To address this need, this research proposes a framework of dynamic capabilities and its microfoundations to assess the opportunities and challenges regarding digital transformation, involving three dimensions: Digital sensing, digital seizing, and digital reconfiguring.Design/methodology/approachThis study employed a descriptive qualitative empirical approach, encompassing a sample of eight companies. Data triangulation was achieved through a combination of in-depth semi-structured interviews and secondary data.FindingsThe research provides evidence that data-driven culture fosters digital transformation and proposes the following new microfoundations: “Analytics for the customer experience journey” and “Digital analytics innovation management”, the internal barriers “Leadership without digital skills” and “Lack of strategic human resources management for digital transformation”, and the internal enablers “Defined strategy for digital transformation” and “Data-driven culture”. Finally, based on empirical results, it was possible to gather clues that link dynamic capabilities with digital maturity.Practical implicationsThe application of the proposed framework in companies enables them to develop a roadmap for the digital transition oriented towards their business and management strategies.Originality/valueThis paper contributes to the literature on dynamic capabilities for digital transformation by proposing new theoretical constructs that unearth their microfoundations, barriers, and enablers.
Turbo-RANS: straightforward and efficient Bayesian optimization of turbulence model coefficients
Authors: McConkey Ryley , Kalia Nikhila , Yee Eugene , Lien Fue-Sang 
Published: June 2024

Purpose
Industrial simulations of turbulent flows often rely on Reynolds-averaged Navier-Stokes (RANS) turbulence models, which contain numerous closure coefficients that need to be calibrated. This paper aims to address this issue by proposing a semi-automated calibration of these coefficients using a new framework (referred to as turbo-RANS) based on Bayesian optimization.

Design/methodology/approach
The authors introduce the generalized error and default coefficient preference (GEDCP) objective function, which can be used with integral, sparse or dense reference data for the purpose of calibrating RANS turbulence closure model coefficients. Then, the authors describe a Bayesian optimization-based algorithm for conducting the calibration of these model coefficients. An in-depth hyperparameter tuning study is conducted to recommend efficient settings for the turbo-RANS optimization procedure.

Findings
The authors demonstrate that the performance of the k-ω shear stress transport (SST) and generalized k-ω (GEKO) turbulence models can be efficiently improved via turbo-RANS, for three example cases: predicting the lift coefficient of an airfoil; predicting the velocity and turbulent kinetic energy fields for a separated flow; and, predicting the wall pressure coefficient distribution for flow through a converging-diverging channel.

Originality/value
To the best of the authors’ knowledge, this work is the first to propose and provide an open-source black-box calibration procedure for turbulence model coefficients based on Bayesian optimization. The authors propose a data-flexible objective function for the calibration target. The open-source implementation of the turbo-RANS framework includes OpenFOAM, Ansys Fluent, STAR-CCM+ and solver-agnostic templates for user application.
Perceived impacts of the COVID-19 pandemic: does farm households' capability influence food security resilience in Ghana?
Authors: Cobbinah Esther , Alhassan Hamdiyah , Daadi Bundom Edward 
Published: June 2024

PurposeCOVID-19 which became a global pandemic in 2020 has had tremendous impacts on several sectors including agriculture. Farm household capability is said to play a major role in ensuring that individuals develop some level of resilience towards the pandemic. This study assesses the perceived impacts of the pandemic on livelihood activities and examines the effects of farm household capability on food security resilience.Design/methodology/approachThe factor analysis, 2SLS and RIMA methodology were employed to assess the perceived impacts of the pandemic on livelihood activities and examine the effects of farm households' capability on food security resilience. The study is based on farm households in the Northern region of Ghana.FindingsThe study revealed that on a scale of 0 (no impact of the COVID-19 pandemic) to 1 (high impact of the COVID-19 pandemic), a value of 0.641 was obtained which means that the COVID-19 pandemic had a moderate (average) impact on farm households. Farm household capability also increases the food security resilience of farm households.Practical implicationsGovernment and local leaders should ensure that basic life-sustaining conditions are provided for rural farmers, and farm household capabilities are considered during crises.Originality/valueStudies have examined impacts of the COVID-19 but little or no attention has been given to farm households' capability to remain resilient amid the COVID-19 shocks on livelihoods.
Education Briefing: A review of problematic investment selection issues associated with IRR and NPV benchmarks
Authors: Larsen James E. 
Published: June 2024

PurposeThe aim of this Education Briefing is to comment on the problematic issues that sometimes arise when using the internal rate of return (IRR) and/or the net present value (NPV) as a measure of expected investment performance. The briefing looks at the sometimes conflicting signposts that each benchmark presents and highlights ways that decision-makers can overcome or mitigate the effects of those problematic issues.Design/methodology/approachAfter a short review of the IRR and NPV techniques, this Education Briefing provides numerous examples of problematic issues that arise with certain cash flow profiles and suggests how to address them.FindingsBoth the IRR and NPV provide simple benchmarks that can mislead the decision-maker who is not familiar with the nuances of both techniques.Practical implicationsThis review should heighten the reader’s ability to spot characteristics of proposed investments that may signal that a quick decision based on performance metrics may lead to disappointing results. These characteristics include: scale effects, unusual cash flow patterns and/or investments with dissimilar expected lives. Mutually exclusive investments merit special attention.Originality/valueThis is a review of existing performance measurement models.
Regenerative adventure tourism. Going beyond sustainability – a horizon 2050 paper
Authors: Schmidt Rojas Nadine , Sand Manuel S. , Gross Sven 
Published: June 2024

Purpose
This study aims to provide an overview of the regenerative concept and how this can be implemented in adventure travel. It looks into the history of sustainable adventure tourism and showcases best practice examples. This study is encouraging operators within the adventure tourism industry to adopt a regenerative approach.

Design/methodology/approach
A comprehensive literature review on the paradigm of regenerative tourism has been conducted and applied to the adventure tourism industry. Three case studies of selected adventure tourism operators have been selected to enforce this concept.

Findings
With nature being an essential element of adventure travel, the industry has a long history in terms of sustainability. While tour operators, destination management organisations and other stakeholders are working towards sustainable products, consumers are still convenient and not willing to restrict themselves. The attitude behaviour gap is also noticeable in this segment and a rethinking is necessary. Through the pandemic and the climate crisis, among other aspects, a shift within the tourism industry is inevitable. Adventure tourism can be a role model in this process and good examples from the industry give hope for change. A concept that focuses on the solutions to address the polycrises is regenerative development, which is based on an ecological worldview and a living systems thinking and aims to restore locally visited communities and environments. Numerous operators have already taken measures to help local communities, such as starting projects for nature conservation or encouraging social justice.

Research limitations/implications
This is a conceptual review without an empirical foundation. The best-case examples are based on an Anglo-Western perspective.

Originality/value
To the best of the authors’ knowledge, this study is the first to provide an overview of the current state of research into sustainable adventure tourism and categorise it within the concept of regenerative tourism.