bluuebunny/crossref_metadata_2025_split huggingface dataset:
A comparison of depression, anxiety, and health status in patients with progressive supranuclear palsy and multiple system atrophy
Authors: Schrag Anette , Sheikh Shamira , Quinn Niall P. , Lees Andrew J. , Selai Caroline , Mathias Chris , Litvan Irene , Lang Anthony E. , Bower James H. , Burn David J. , Low Philip , Jahanshahi Marjan 
Published: April 2010

AbstractThe objective of this study was to compare subjective health status and its correlates in progressive supranuclear palsy (PSP) and multiple system atrophy (MSA). One hundred eighty‐eight patients with PSP and 286 patients with MSA completed EQ‐5D and Hospital Depression and Anxiety Scale. The impact on mobility, usual activities, and self‐care was similarly high in both groups after similar duration. Fifty‐six percent of PSP and 43% of MSA had probable depression, and 37% of both groups had probable anxiety. Patients with PSP had significantly higher depression scores, but groups did not differ in anxiety scores. Patients with MSA had significantly greater pain/discomfort than patients with PSP. The most important association with subjective health status was with depressive symptoms, which accounted for 38% and 29% of EQ‐5D variance in patients with PSP and MSA, followed by disease severity and anxiety scores. We conclude that depressive symptoms were common in both disorders, but more severe in PSP. Anxiety symptoms affected 37% of patients in both groups and contributed to impaired subjective health status. Pain was more problematic in MSA than PSP. © 2010 Movement Disorder Society
Spinocerebellar ataxia type 12 identified in two Italian families may mimic sporadic ataxia
Authors: Brussino Alessandro , Graziano Claudio , Giobbe Dario , Ferrone Marina , Dragone Elisa , Arduino Carlo , Lodi Raffaele , Tonon Caterina , Gabellini Anna , Rinaldi Rita , Miccoli Sara , Grosso Enrico , Bellati Maria Cristina , Orsi Laura , Migone Nicola , Brusco Alfredo 
Published: April 2010

AbstractSCA12 is an autosomal dominant cerebellar ataxia characterized by onset in the fourth decade of life with action tremor of arms and head, mild ataxia, dysmetria, and hyperreflexia. The disease is caused by an expansion of ≥51 CAGs in the 5′ region of the brain‐ specific phosphatase 2 regulatory subunit B‐beta isoform (PPP2R2B) gene. SCA12 is very rare, except for a single ethnic group in India. We screened 159 Italian ataxic patients for SCA12 and identified two families that segregated an expanded allele of 57 to 58 CAGs, sharing a common haplotype. The age at onset, phenotype, and variability of symptoms were compatible with known cases. In one family, the disease was apparently sporadic due to possible incomplete penetrance and/or late age at onset. Our data indicate that SCA12 is also present in Italian patients, and its genetic testing should be applied to both sporadic and familial ataxias. © 2010 Movement Disorder Society
A novel X‐linked four‐repeat tauopathy with Parkinsonism and spasticity
Authors: Poorkaj Parvoneh , Raskind Wendy H. , Leverenz James B. , Matsushita Mark , Zabetian Cyrus P. , Samii Ali , Kim Sophia , Gazi Nayiry , Nutt John G. , Wolff John , Yearout Dora , Greenup J. Lynne , Steinbart Ellen J. , Bird Thomas D. 
Published: April 2010

AbstractThe parkinsonian syndromes comprise a highly heterogeneous group of disorders. Although 15 loci are linked to predominantly familial Parkinson's disease (PD), additional PD loci are likely to exist. We recently identified a multigenerational family of Danish and German descent in which five males in three generations presented with a unique syndrome characterized by parkinsonian features and variably penetrant spasticity for which X‐linked disease transmission was strongly suggested (XPDS). Autopsy in one individual failed to reveal synucleinopathy; however, there was a significant four‐repeat tauopathy in the striatum. Our objective was to identify the locus responsible for this unique parkinsonian disorder. Members of the XPDS family were genotyped for markers spanning the X chromosome. Two‐point and multipoint linkage analyses were performed and the candidate region refined by analyzing additional markers. A multipoint LODmax score of 2.068 was obtained between markers DXS991 and DXS993. Haplotype examination revealed an ∼20 cM region bounded by markers DXS8042 and DXS1216 that segregated with disease in all affected males and obligate carrier females and was not carried by unaffected at‐risk males. To reduce the possibility of a false‐positive linkage result, multiple loci and genes associated with other parkinsonian or spasticity syndromes were excluded. In conclusion, we have identified a unique X‐linked parkinsonian syndrome with variable spasticity and four‐repeat tau pathology, and defined a novel candidate gene locus spanning ∼28 Mb from Xp11.2–Xq13.3. © 2010 Movement Disorder Society
Timed motor tests can detect subtle motor dysfunction in early Parkinson's disease
Authors: Haaxma Charlotte A. , Bloem Bastiaan R. , Overeem Sebastiaan , Borm George F. , Horstink Martin W.I.M. 
Published: April 2010

AbstractEarly diagnosis of Parkinson's disease (PD) is important for putative neuroprotective therapies to be initiated in the earliest stage of the disease. We investigated whether a previously validated timed motor test (TMT) battery could detect subtle motor dysfunction in early PD patients and even in clinically unaffected limbs of strictly hemiparkinsonian patients. We assessed 107 PD patients (symptom duration ≤2 years; dopa‐naive) and 100 healthy, age‐matched controls with eight simple TMTs based on aspects of (a) walking, (b) writing, (c) single and double‐handed pegboard performance, (d) finger tapping, and (e) diadochokinesis. We evaluated the ability of individual and combined TMTs to discriminate patients from controls using ROC curves. Second, we investigated whether these TMTs could identify motor dysfunction of the clinically unaffected limb in 42 strictly hemiparkinsonian patients. The pegboard dexterity test had the best ROC curve (AUC 0.97; 95% sensitivity, 89% specificity) for patients versus controls. It retained reasonable accuracy when testing the clinically unaffected limb of hemiparkinsonian patients versus the mean of right and left‐hand scores in controls (AUC 0.73). The pegboard dexterity test is a sensitive and inexpensive instrument to detect motor dysfunction in early PD. Therefore, it may be worth evaluating as a diagnostic tool in everyday clinical practice to assess patients with early symptomatic PD, or as part of a more elaborate screening battery in a defined population at risk. © 2010 Movement Disorder Society
Statin use and Parkinson's disease in Denmark
Authors: Ritz Beate , Manthripragada Angelika D. , Qian Lei , Schernhammer Eva , Wermuth Lene , Olsen Jorgen , Friis Soren 
Published: April 2010

AbstractThe objective of this study was to investigate whether statin (3‐hydroxy‐3‐methylglutaryl‐coenzyme A reductase inhibitor) use is associated with risk of Parkinson's disease (PD) in Denmark. We identified 1,931 patients with a first time diagnosis of PD reported in hospital or outpatient clinic records between 2001 and 2006. We density matched to these patients 9,651 population controls by birth year and sex relying on the Danish population register. For every participant, we identified pharmacy records of statin and anti‐Parkinson drug prescriptions since 1995 and before index date from a prescription medication use database for all Danish residents. Whenever applicable, the index dates for cases and their corresponding controls were advanced to the date of first recorded prescription for anti‐Parkinson drugs. In our primary analyses, we excluded all statin prescriptions 2‐years before PD diagnosis. Employing logistic regression adjusting for age, sex, diagnosis of chronic obstructive pulmonary disease, and Charlson comorbidity, we observed none to slightly inverse associations between PD diagnosis and statin prescription drug use. Inverse associations with statin use were only observed for short‐term (≤1 yrs) statin users (2‐year lag OR 0.57; 95% CI 0.36 to 0.89); and suggested at higher intensity statin use (2‐year lag OR 0.69; 95% CI 0.45–1.04). No associations were seen among long‐term users and no difference by sex, age, or type of statins used (lipophilic/hydrophilic). We found little evidence for a neuroprotective role of statins in PD except for short‐term or high intensity users. Yet, further investigations into the contributions of intensity, duration, and lag periods of statin use may still be warranted. © 2010 Movement Disorder Society
Influence of deep brain stimulation and levodopa on sensory signs in Parkinson's disease
Authors: Gierthmühlen Janne , Arning Philipp , Binder Andreas , Herzog Jan , Deuschl Günther , Wasner Gunnar , Baron Ralf 
Published: April 2010

AbstractTo examine the effects of levodopa (L‐dopa) and deep brain stimulation of the subthalamic nucleus (STN‐DBS) on sensory symptoms and signs in Parkinson's disease (PD). Seventeen patients with PD were included. (1) Presence of sensory symptoms and (2) effects of L‐dopa and STN‐DBS on sensory symptoms and signs [assessed by quantitative sensory testing (QST)] were examined 6 months after starting STN‐DBS. In addition, in 12 of these patients, presence of sensory symptoms prior and post STN‐DBS was compared. Pain was most frequently nociceptive. In about 30–40%, pain and sensory symptoms were associated with PD motor symptoms. In most of these cases, pain responded to L‐dopa. Intensity of pain was reduced post STN‐DBS compared to pre STN‐DBS. L‐Dopa had no influence on detection thresholds, whereas STN‐DBS improved thermal detection thresholds. However, thermal and mechanical pain thresholds were uninfluenced by L‐dopa or STN‐DBS. Although some patients reported an improvement of pain with STN‐DBS or L‐dopa, objectively pain sensitivity as assessed by QST was not altered by STN‐DBS or L‐dopa suggesting that there is no evidence for a direct modulation of central pain processing by L‐dopa or STN‐DBS. © 2010 Movement Disorder Society
Coenzyme Q10‐responsive ataxia: 2‐year‐treatment follow‐up
Authors: Pineda Merce , Montero Raquel , Aracil Asuncion , O'Callaghan Mar M. , Mas Ana , Espinos Carmen , Martinez‐Rubio Dolores , Palau Francesc , Navas Placido , Briones Paz , Artuch Rafael 
Published: April 2010

AbstractWe assessed the clinical outcome after coenzyme Q10 (CoQ10) therapy in 14 patients presenting ataxia classified into two groups according to CoQ10 values in muscle (deficient or not). We performed an open‐label prospective study: patients were evaluated clinically (international cooperative ataxia rating scale [ICARS] scale, MRI, and videotape registration) at baseline and every 6 months during a period of 2 years after CoQ10 treatment (30 mg/kg/day). Patients with CoQ10 deficiency showed a statistically significant reduction of ICARS scores (Wilcoxon test: P = 0.018) after 2 years of CoQ10 treatment when compared with baseline conditions. In patients without CoQ10 deficiency, no statistically significant differences were observed in total ICARS scores after therapy, although 1 patient from this group showed a remarkable clinical amelioration. Biochemical diagnosis of CoQ10 deficiency was a useful tool for the selection of patients who are good candidates for treatment as all of them responded to therapy. However, the remarkable clinical response in 1 case without CoQ10 deficiency highlights the importance of treatment trials for identification of patients with CoQ10‐responsive ataxia. © 2010 Movement Disorder Society
Synthesis and thermal properties of antimony doped tin oxide/waterborne polyurethane nanocomposite films as heat insulating materials
Authors: Dai Zhen , Li Zhihua , Li Li , Xu Gewen 
Published: April 2010

AbstractThe waterborne polyurethane (WPU) was synthesized from the polycondensation between isophorone diisocyanate (IPDI) and polyoxypropylene glycol (N‐210) and then dispersed into water. Subsequently, the WPU emulsion was modified with antimony doped tin oxide (ATO) nanoparticle by ultrasonic dispersion. The ATO/WPU emulsion was cast onto Teflon molds. After being dried, ATO/WPU films were prepared. TEM indicated that the ATO nanoparticles were homogeneously dispersed in the polymer matrix at the nanometer scale. DSC showed that the ATO/WPU nanocomposites displayed increased glass transition temperatures compared to the control WPU. The mechanical properties of the films were characterized by dynamic‐mechanical analysis (DMA). The higher glass transition temperature and storage modulus indicates the superior mechanical properties of WPU modified by ATO nanoparticles over the conventional unmodified WPU. The thermal behaviors of the films were evaluated by thermogravimetric analysis (TGA). It could be found that the incorporation of ATO into WPU can improve the thermal stability dramatically. The results from UV–visible–near infrared spectra indicated that the ATO/WPU films could decrease the infrared transmission effectively. The heat‐insulation measurements showed that glass coated with ATO/WPU films possessed better heat‐insulating effect than empty glass. Copyright © 2010 John Wiley & Sons, Ltd.
Neuroblastoma of unknown primary site with periorbital bone metastasis in a child
Authors: Salmi Darren , Patel Chirag , Imashuku Shinsaku , Shimada Hiroyuki , Satake Noriko 
Published: April 2010

AbstractNeuroblastoma is the second most common solid tumor in children. Most tumors arise in the adrenal glands or paravertebral region. Rarely, patients present with metastatic disease but no primary site can be found despite extensive imaging. We report here a patient with a large periorbital bone metastasis and bone marrow involvement but with no known primary site. Pediatr Blood Cancer. © 2010 Wiley‐Liss, Inc.
Randomized trial to compare LSA2L2‐type maintenance therapy to daily 6‐mercaptopurine and weekly methotrexate with vincristine and dexamethasone pulse for children with acute lymphoblastic leukemia
Authors: Nagatoshi Yoshihisa , Matsuzaki Akinobu , Suminoe Aiko , Inada Hiroko , Ueda Kouichiro , Kawakami Kiyoshi , Yanai Fumio , Nakayama Hideki , Moritake Hiroshi , Itonaga Nobuyoshi , Hotta Noriko , Fujita Kyoko , Hidaka Yasufumi , Yamanaka Takeharu , Kawano Yoshifumi , Okamura Jun 
Published: April 2010

AbstractBackgroundA total of 201 pediatric cases of acute lymphoblastic leukemia were treated with the ALL‐96 protocol by the Kyushu‐Yamaguchi Children's Cancer Study Group.ProcedureRisk stratification was based on white cell counts, immunophenotype, the presence of central nervous system disease at diagnosis, organomegaly, and early treatment response (day 14 bone marrow status). All of the patients were classified into standard‐risk (SR) or high‐risk (HR) groups and were randomly assigned to receive maintenance therapy with either LSA2L2‐type or 6‐mercaptopurine (6‐MP)/methotrexate (MTX) with vincristine (VCR) and dexamethasone (DEX) pulse in both risk groups.ResultsThe 7‐year event‐free survival (EFS) and overall survival (OS) rates in the entire study population were 72.1% (95% CI: 68.0–76.2%) and 84.8% (95% CI: 79.7–89.9%), respectively, and the EFS of the SR patients (85.3% [95% CI: 78.2–92.4%]) was significantly better than HR patients (62.4% [95% CI: 52.2–72.6%]) (P = 0.0007).ConclusionsThere were no differences in the EFS between the different maintenance therapies in each risk group; however, grade IV liver toxicity occurred more often in the patients receiving 6‐MP/MTX with VCR and DEX therapy than in patients receiving LSA2L2. Pediatr Blood Cancer. © 2010 Wiley‐Liss, Inc.
Review of polymer materials with low dielectric constant
Authors: Zhao Xiong‐Yan , Liu Hong‐Jie 
Published: April 2010

AbstractOne of the exciting and promising developments in material science today is the design and synthesis of novel low‐dielectric‐constant polymer materials, which are found to have potential applications in the field of ultralarge‐scale integration, capacitors and other electronic circuits as insulating and/or dielectric materials. In this article the new polymer dielectric materials reported in recent years are reviewed, including aromatic (heteroaromatic) polymers, silicon‐containing polymers, fluorinated polymers, porous polymers, etc. In summarizing the review, the development, potential applications and future directions of polymer materials with low dielectric constant are discussed. Copyright © 2010 Society of Chemical Industry
Differential proteomic analysis using isotope‐coded protein‐labeling strategies: Comparison, improvements and application to simulated microgravity effect on Cupriavidus metallidurans CH34
Authors: Leroy Baptiste , Rosier Caroline , Erculisse Vanessa , Leys Natalie , Mergeay Max , Wattiez Ruddy 
Published: April 2010

AbstractAmong differential proteomic methods based on stable isotopic labeling, isotope‐coded protein labeling (ICPL) is a recent non‐isobaric technique devised to label primary amines found in proteins. ICPL overcomes some of the disadvantages found in other chemical‐labeling techniques, such as iTRAQ or ICAT. However, previous analyses revealed that more than 30% of the proteins identified in regular ICPL generally remain unquantified. In this study, we describe a modified version of ICPL, named Post‐digest ICPL, that makes it possible to label and thus to quantify all the peptides in a sample (bottom–up approach). Optimization and validation of this Post‐digest ICPL approach were performed using a standard protein mixture and complex protein samples. Using this strategy, the number of proteins that were identified and quantified was greatly increased in comparison with regular ICPL and cICAT approaches. The pros and cons of this improvement are discussed. This complementary approach to traditional ICPL was applied to the analysis of modification of protein abundances in the model bacterium Cupriavidus metallidurans CH34 after cultivation under simulated microgravity. In this context, two different systems – a 2‐D clinorotation and 3‐D random positioning device – were used and the results were compared and discussed.
Protein reference mapping of dihydrofolate reductase‐deficient CHO DG44 cell lines using 2‐dimensional electrophoresis
Authors: Lee Jae Seong , Park Hyun Ju , Kim Young Hwan , Lee Gyun Min 
Published: April 2010

AbstractChinese hamster ovary (CHO) cells are the major mammalian host for producing various therapeutic proteins. Among CHO cells, the dihydrofolate reductase‐deficient CHO DG44 cell line has been used as a popular mammalian host because of the availability of a well‐characterized genetic selection and amplification system. However, this cell line has not been studied at the proteome level. Here, the first detailed proteome analysis of the CHO DG44 cell line is described. A protein reference map of the CHO DG44 cell line was established by analyzing whole cellular proteins using 2‐DE with various immobilized pH gradients (pHs 3–10, 5–8, and 3–6) in the first dimension and a 12% acrylamide gel in the second dimension. The map is composed of over 1400 silver‐stained protein spots. Among them, 179 protein spots, which represent proteins associated with various biological processes and cellular compartments, were identified based on MALDI‐TOF‐MS and MS/MS. This proteome database should be valuable for better understanding of CHO cell physiology and protein expression patterns which may lead to efficient therapeutic protein production.
Analysis of hepatic glycogen‐associated proteins
Authors: Stapleton David , Nelson Chad , Parsawar Krishna , McClain Donald , Gilbert‐Wilson Ryan , Barker Elizabeth , Rudd Brant , Brown Kevin , Hendrix Wayne , O'Donnell Paul , Parker Glendon 
Published: April 2010

AbstractGlycogen particles are associated with a population of proteins that mediate its biological functions, including: management of glucose flux into and out of the glycogen particle, maintenance of glycogen structure and regulation of particle size, number, and cellular location. A survey of the glycogen‐associated proteome would be predicted to identify the relative representation of known members of this population, and associations with unexpected proteins that have the potential to mediate other functions of the glycogen particle. We therefore purified glycogen particles from both mouse and rat liver, using different techniques, and analyzed the resulting tryptic peptides by MS. We also specifically eluted glycogen‐binding proteins from the pellet using malto‐oligosaccharides. Comparison of the rat and mouse populations, and analysis of specifically eluted proteins allow some conclusions to be made about the hepatic glycogen sub‐proteome. With the exception of glycogen branching enzyme all glycogen metabolic proteins were detected. Novel associations were identified, including ferritin and starch‐binding domain protein 1, a protein that contains both a transmembrane endoplasmic reticulum signal peptide and a carbohydrate‐binding module. This study therefore provides insight into the organization of the glycogen proteome, identifies other associated proteins and provides a starting point to explore the dynamic nature and cellular distribution of this metabolically important protein population.
The essentiality of α‐2‐macroglobulin in human salivary innate immunity against new H1N1 swine origin influenza A virus
Authors: Chen Chao‐Hsuan , Zhang Xing‐Quan , Lo Chih‐Wei , Liu Pei‐Feng , Liu Yu‐Tsueng , Gallo Richard L. , Hsieh Ming‐Fa , Schooley Robert T. , Huang Chun‐Ming 
Published: April 2010

AbstractA novel strain of influenza A H1N1 emerged in the spring of 2009 and has spread rapidly throughout the world. Although vaccines have recently been developed that are expected to be protective, their availability was delayed until well into the influenza season. Although anti‐influenza drugs such as neuraminidase inhibitors can be effective, resistance to these drugs has already been reported. Although human saliva was known to inhibit viral infection and may thus prevent viral transmission, the components responsible for this activity on influenza virus, in particular, influenza A swine origin influenza A virus (S‐OIV), have not yet been defined. By using a proteomic approach in conjunction with beads that bind α‐2,6‐sialylated glycoprotein, we determined that an α‐2‐macroglobulin (A2M) and an A2M‐like protein are essential components in salivary innate immunity against hemagglutination mediated by a clinical isolate of S‐OIV (San Diego/01/09 S‐OIV). A model of an A2M‐based “double‐edged sword” on competition of α‐2,6‐sialylated glycoprotein receptors and inactivation of host proteases is proposed. We emphasize that endogenous A2M in human innate immunity functions as a natural inhibitor against S‐OIV.
Proteomic analysis reveals novel binding partners of MIP‐T3 in human cells
Authors: Guo Chao‐Wan , Xiong Sheng , Liu Ge , Wang Yi‐Fei , He Qing‐Yu , Zhang Xian‐En , Zhang Zhi‐Ping , Ge Feng , Kitazato Kaio 
Published: April 2010

AbstractMIP‐T3 (microtubule‐interacting protein associated with TRAF3) is a microtubule‐interacting protein that evolutionarily conserved from worms to humans, but whose cellular functions remains unknown. To get insight into the functions of MIP‐T3, we set out to identify MIP‐T3 interacting proteins by immunoprecipitation in human embryonic kidney 293 cells and MS analysis. As the results, a total of 34 proteins were identified and most of them were novel MIP‐T3 putative partners. The MIP‐T3‐associated proteins could be grouped into nine clusters based on their molecule functions, including cytoskeleton, chaperone, nucleic acid binding, kinase and so on. Three MIP‐T3‐interacted proteins – actin, HSPA8 and tubulin – were further confirmed by reciprocal coimmunoprecipitations and colocalization analysis. The interaction of MIP‐T3 with both actin filaments and microtubule suggested that MIP‐T3 may play an important role in regulation of cytoskeleton dynamics in cells. Our results therefore not only uncover a large number of MIP‐T3‐associated proteins that possess a variety of cellular functions, but also provide new research directions for the study of the functions of MIP‐T3.