bluuebunny/crossref_metadata_2025_split huggingface dataset:
An inducible model of abacterial prostatitis induces antigen specific inflammatory and proliferative changes in the murine prostate
Authors: Haverkamp Jessica M. , Charbonneau Bridget , Crist Scott A. , Meyerholz David K. , Cohen Michael B. , Snyder Paul W. , Svensson Robert U. , Henry Michael D. , Wang Hsing‐Hui , Ratliff Timothy L. 
Published: January 2011

AbstractBACKGROUNDProstatitis is a poorly understood disease and increasing evidence suggests inflammation is involved in other prostatic diseases including prostate cancer.METHODSThe ability of pre‐activated CD8 T cells to induce prostatitis was examined by adoptive transfer of prostate antigen specific CD8 T cells into POET‐3 mice or POET‐3/Luc/Pten−/+ mice. Characterization of the inflammatory response was determined by examining leukocyte infiltration by histological analysis, flow cytometry and by evaluating cytokine and chemokine levels in prostate tissue. The impact of inflammation on the prostate was evaluated by monitoring epithelial cell proliferation over time.RESULTSInitiation of inflammation by ovalbumin specific CD8+ T cells (OT‐I cells) resulted in development of acute prostatitis in the anterior, dorsolateral and ventral prostate of POET‐3 and POET‐3/Luc/Pten−/+ mice. Acute prostatitis was characterized by recruitment of adoptively transferred OT‐I cells and importantly, autologous CD4+ and CD8+ T cells, myeloid‐derived suppressor cells (MDSC) and regulatory T cells (Treg). In concert with leukocyte infiltration elevated levels of pro‐inflammatory cytokines and chemokines were observed. Inflammation also resulted in marked epithelial cell proliferation that was sustained up to 80 days post adoptive transfer of OT‐I cells.CONCLUSIONSThe POET‐3 model represents a novel mouse model to study both acute and chronic prostate inflammation in an antigen‐specific system. Further, the POET‐3 mouse model can be crossed with other genetic models of disease such as the C57/Luc/Pten−/− model of prostate cancer, allowing the impact of prostatitis on other prostatic diseases to be evaluated. Prostate 71:1139–1150, 2011. © 2011 Wiley‐Liss, Inc.
Functional polymorphisms in cell death pathway genes FAS and FAS ligand and risk of prostate cancer in a Chinese population
Authors: Shao Pengfei , Ding Qi , Qin Chao , Wang Meilin , Tang Jialin , Zhu Jian , Chen Jiawei , Cao Qiang , Li Jie , Xu Bin , Zhang Zhengdong , Zhang Wei , Yin Changjun 
Published: January 2011

AbstractBACKGROUNDApoptosis is the physiological mechanism of programmed cell death and abnormal regulation of this pathway can lead to carcinogenesis. We hypothesized that the FAS −1377G>A, −670A>G, and FASL −844T>C polymorphisms are associated with risk of prostate cancer (PCa).MATERIALS AND METHODSWe genotyped polymorphisms in a hospital‐based case–control study of 602 patients diagnosed with PCa and 703 cancer‐free controls in a Chinese population using polymerase chain reaction‐restriction fragment length polymorphism method.RESULTSWe found a significantly decreased risk associated with FAS −1377GA [adjusted odds ratio (OR) = 0.71, 95% confidence interval (CI) = 0.56–0.90] genotype compared with the −1377GG genotype and decreased risk associated with FAS −670AG and −670GG genotypes (OR 0.75, 95% CI: 0.59–0.95; OR 0.70, 95% CI: 0.50–0.96) compared with the −670AA genotype. Consistently, we found that individuals carrying haplotype genotype with 0 or 1 risk allele (−1377G and −670A) had a lower risk of PCa than those with two risk alleles (OR 0.48, 95% CI: 0.28–0.80; OR 0.60, 95% CI: 0.41–0.87; OR 0.82, 95% CI: 0.69–0.97 for the AA, GG, and AG haplotype, respectively). In addition, when we evaluated these two FAS polymorphisms together, we found that the combined genotype with 4 risk alleles was associated with a significantly increased risk of PCa compared with those with 0–3 variants (OR 1.51, 95% CI: 1.19–1.91), and this increased risk was more pronounced among subgroups of Gleason score <7 and >7, and PSA > 20 ng/ml (OR 1.49, 95% CI: 1.08–2.04; OR 1.72, 95% CI: 1.25–2.44; OR 1.61, 95% CI: 1.23–2.13, respectively).CONCLUSIONSThese results suggested that the FAS polymorphisms may contribute to PCa risk in a Chinese population. Prostate 71:1122–1130, 2011. © 2011 Wiley‐Liss, Inc.
Resistance to docetaxel‐induced apoptosis in prostate cancer cells by p38/p53/p21 signaling
Authors: Gan Lu , Wang Jianlin , Xu Huibi , Yang Xiangliang 
Published: January 2011

AbstractBACKGROUNDTaxane chemotherapy is one of the few therapeutic options for men with castration‐resistant prostate cancer. However, the working mechanisms are not fully understood. We aimed to investigate the possible molecular mechanism of apoptosis induced by taxanes in prostate cancer.METHODSThe human LNCaP cells (bearing wild‐type p53), DU145 cells (bearing mutant p53) and PC3 cells (lacking p53) were used. The expression levels of protein were determined by Western blot and the mRNA levels were determined by reverse transcriptase PCR. The apoptosis was measured by propidium iodide (PI) staining and flow cytometric analysis.RESULTSLNCaP cells are more resistant to docetaxel than DU145 and PC3 cells. Knocking down p53 by small interference RNA (siRNA) sensitizes LNCaP cells to docetaxel treatment. Docetaxel stabilizes p53 protein level and upregulates p21 in a p53‐dependent manner in LNCaP cells. Docetaxel increases p38 phosphorylation in LNCaP cells. Treatment with p38‐specific inhibitor SB203580 or knocking down p38 by siRNA significantly impaired the upregulation of p53 and p21 by docetaxel. Knocking down p38 or p21 sensitizes LNCaP cells to docetaxel treatment and the antiapoptotic effect of p21 can be reversed by p38 siRNA in LNCaP cells.CONCLUSIONSStimulation of the p38/p53/p21 signaling axis could be important for regulating the susceptibility towards docetaxel in prostate cancer. Prostate 71:1158–1166, 2011. © 2011 Wiley‐Liss, Inc.
CYP17 gene polymorphisms and prostate cancer risk: A meta‐analysis based on 38 independent studies
Authors: Wang Fang , Zou Yan‐Feng , Feng Xiao‐Liang , Su Hong , Huang Fen 
Published: January 2011

AbstractBACKGROUNDThe results of recent published studies focusing on CYP17 polymorphisms in prostate cancer (PCa) susceptibility are often conflicting. We performed a meta‐analysis based on 38 independent studies to evaluate the association.METHODSData were collected from the following electronic databases: PubMed, Excerpta Medica Database, and Chinese Biomedical Literature Database, with the last report up to September 2010. Meta‐analysis was conducted in a fixed/random effect model.RESULTSThirty‐eight independent studies including 34,782 cases and 38,626 controls on the association of CYP17 gene polymorphisms with PCa risk in different ethnic groups were identified. The meta‐analysis was performed for five polymorphisms: rs743572 (A1/A2, 38 studies), rs6162 (C/T, 3 studies), rs619824 (C/A, 4 studies), rs2486758 (T/C, 4 studies), and rs10883782 (A/G, 4 studies). When all groups were pooled, we did not detect the association of rs743572 polymorphism with PCa risk. In the subgroup analysis, a significant association of rs743572 polymorphism and PCa was found in Black population (A2/A2 vs. A1/A1 + A2/A1: OR = 1.70, 95% CI = 1.08–2.69, P = 0.02), but not in Caucasian or Asian population. For other polymorphisms, we found that rs619824 polymorphism was associated with a significant decreased risk of PCa (A vs. C: OR = 0.95, 95% CI = 0.92–0.99, P = 0.01), and rs2486758 polymorphism was associated with a significant increased risk of PCa (C vs. T: OR = 1.07, 95% CI = 1.03–1.12, P = 0.002).CONCLUSIONThis meta‐analysis suggests that rs743572 polymorphism is associated with PCa risk in Black population, but not in Caucasian or Asian population. Moreover, our study suggests that rs619824 and rs2486758 polymorphisms are associated with PCa risk. Prostate 71:1167–1177, 2011. © 2011 Wiley‐Liss, Inc.
Impact of prostate‐specific antigen (PSA) nadir and time to PSA nadir on disease progression in prostate cancer treated with androgen‐deprivation therapy
Authors: Huang Shu‐Pin , Bao Bo‐Ying , Wu Ming‐Tsang , Choueiri Toni K. , Goggins William B. , Huang Chao‐Yuan , Pu Yeong‐Shiau , Yu Chia‐Cheng , Huang Chun‐Hsiung 
Published: January 2011

AbstractBACKGROUNDThe influence of PSA kinetics on the outcome of metastatic prostate cancer after androgen deprivation therapy (ADT) is not well understood. We evaluated the prognostic significance of PSA nadir and time to PSA nadir as well as their potential interactive effect on the progression of disease after ADT.METHODSA total of 650 men with advanced or metastatic prostate cancer treated with ADT were studied. The prognostic significance of PSA nadir and time to PSA nadir on disease progression were analyzed using Kaplan–Meier analysis and the Cox regression model.RESULTSWe found that both PSA nadir and time to PSA nadir were independent and significant predictors of disease progression. Patients with higher PSA nadir (≥0.2 ng/ml) and shorter time to PSA nadir (<10 months) had significant shorter time to disease progression after adjusting for other covariates. The combined analyses showed a potential synergistic effect of these two variables on disease progression. Patient with higher PSA nadir and shorter time to PSA nadir had significantly higher risk for disease progression compared to those with lower PSA nadir and longer time to PSA nadir (Hazard Ratios (HR) = 3.11, P < 0.001).CONCLUSIONSWe concluded that both PSA nadir and time to PSA nadir are significant predictors of disease progression for prostate cancer patients receiving ADT. Prostate 71:1189–1197, 2011. © 2011 Wiley‐Liss, Inc.
Parathyroid hormone is not involved in prostate growth in patients with benign prostatic hyperplasia
Authors: Kim Won Tae , Choi Young Deuk , Park Cheol , Kim Young‐Won , Yun Seok Joong , Kim Isaac Yi , Kim Wun‐Jae 
Published: January 2011

AbstractBACKGROUNDA recent population‐based study reported that serum calcium and parathyroid hormone stimulate prostate growth. We evaluated whether serum PTH, vitamin D, and calcium levels correlate with prostate size, PSA levels, and obesity in Korean patients with histologically proven BPH.METHODSPatients with histopathologically proven BPH who underwent transurethral resection of the prostate were enrolled (n = 289). Patients with PSA levels of ≥3 ng/ml underwent multicore transrectal prostate biopsy before TURP to rule out prostate cancer. Patients with serum creatinine levels >1.4 mg/dl, PSA levels >20 ng/ml, and/or PTH levels <10 pg/ml were excluded. Correlations between serum parameters and clinical data were determined. After adjustment for potential confounders, including age and body mass index, multiple linear regression served to compute associations.RESULTSThe mean age, serum PSA level, PTH level, and prostate size were 68.13 ± 7.15 years, 4.10 ± 3.88 ng/ml, 24.33 ± 12.52 pg/ml, and 44.27 ± 24.15 g, respectively. Prostate size correlated positively with age (r = 0.209, P < 0.001) and PSA levels (r = 0.481, P < 0.001), and PSA levels correlated positively with age (r = 0.226, P < 0.001) and prostate size (r = 0.481, P < 0.001), but neither variable correlated with PTH, vitamin D, calcium levels, or BMI. Upon multiple adjusted linear regression analysis, prostate size correlated with BMI and serum PSA (both P < 0.001), and serum PSA levels correlated with BMI and prostate size (P = 0.007, P < 0.001, respectively), but neither variable correlated with PTH, vitamin D, or serum calcium levels.CONCLUSIONSIn Korean patients with histopathologically proven BPH, high PTH, vitamin D, and calcium levels do not stimulate prostate growth. Prostate 71:1210–1215, 2011. © 2011 Wiley‐Liss, Inc.
Long‐term cryopreservation of pyramidalis muscle specimens as a source of striated muscle stem cells for treatment of post‐prostatectomy stress urinary incontinence
Authors: Sumino Yasuhiro , Hirata Yuji , Hanada Mari , Akita Yasuyuki , Sato Fuminori , Mimata Hiromitsu 
Published: January 2011

AbstractBACKGROUNDStem‐cell injection into the degenerated external urethral sphincter is a new treatment modality for stress urinary incontinence (SUI). We examined the possibility of long‐term cryopreserved pyramidalis muscle (PM) specimens as a source of striated muscle stem cells for the treatment of post‐prostatectomy SUI.METHODSPM specimens were obtained from five male patients (mean age, 61–70 years) who underwent radical prostatectomy for prostate cancer. Specimens (volume, approximately 125 mm3) were obtained from the incisional edge, minced, and stored at −80°C in a freezing medium (Cell Banker 1®). After 24–60 months, the specimens were thawed and directly incubated at 37°C. Satellite cells were selectively cultured by magnetic affinity cell sorting using an anti‐neural cell adhesion molecule (NCAM) antibody. Osteogenic and adipogenic differentiation were induced by bone morphogenic protein‐7 (BMP‐7) and γ‐linolenic acid, respectively.RESULTSNCAM‐positive cells (>99% purity) were selectively cultured from all cryopreserved PM specimens and confirmed as being of striated muscle origin by the expression of desmin and MyoD. They fused and differentiated into multinucleated myotubes 7 days after incubation in a differentiation induction medium. Stimulation by BMP‐7 and γ‐linolenic acid induced expression of alkaline phosphatase (osteoblast marker) and lipid deposition within the cytoplasm (adipocyte characteristic), respectively.CONCLUSIONSLong‐term cryopreserved PM specimens can be used to culture muscle stem cells. Therefore, this method may be utilized for SUI treatment when necessary. Moreover, complete remove of the prostate gland without fear of injury to the urethral sphincter may be possible in patients with apical cancer or T3 prostate cancer. Prostate 71:1225–1230, 2011. © 2011 Wiley‐Liss, Inc.
TM4SF1, a novel primary androgen receptor target gene over‐expressed in human prostate cancer and involved in cell migration
Authors: Allioli Nathalie , Vincent Séverine , Vlaeminck‐Guillem Virginie , Decaussin‐Petrucci Myriam , Ragage Florence , Ruffion Alain , Samarut Jacques 
Published: January 2011

AbstractBACKGROUNDThe Androgen Receptor (AR) plays a key role in controlling prostate gland homeostasis and contributes to prostate carcinogenesis. The identification of its target genes should provide new candidates that may be implicated in cancer initiation and progression.METHODSTranscriptomic experiments and chromatin immunoprecipitation were combined to identify direct androgen regulated genes. Real‐time quantitative PCR (RT‐qPCR) analyses were performed to measure TM4SF1 mRNA levels in prostate cancer and benign prostatic hyperplasia (BPH) specimens. Immunohistochemical methods were used to compare TM4SF1 protein expression profiles in the same cohort. A targeted siRNAs knockdown strategy was used, prior to wound healing assays, to analyze the role of TM4SF1 in cell migration in vitro.RESULTSWe demonstrate for the first time that TM4SF1 is a direct target gene of the AR, a transcription factor of the steroid nuclear receptor family. A functional androgen response element was identified in the promoter region of the gene. In addition, TM4SF1 mRNA expression was higher in cancer samples compared to BPH tissues. The TM4SF1 protein mediates cell motility of prostate cancer cells where it is predominantly localized in the cytoplasm, in contrast to its apical membrane localization in normal prostate epithelial cells.CONCLUSIONSOur results reveal a novel function for TM4SF1 in AR signaling. The TM4SF1 mRNA expression is higher in prostate cancer tissues as compared to BPH samples. Inhibition of cell migration after targeted knockdown of TM4SF1 protein expression suggests its contribution to prostate cancer cell metastasis. Prostate 71:1239–1250, 2011. © 2011 Wiley‐Liss, Inc.
Epitope motif of an anti‐nitrotyrosine antibody specific for tyrosine‐nitrated peptides revealed by a combination of affinity approaches and mass spectrometry
Authors: Drǎguşanu Mihaela , Petre Brînduşa‐Alina , Przybylski Michael 
Published: January 2011

AbstractNitration of tyrosine residues has been shown to be an important oxidative modification in proteins and has been suggested to play a role in several diseases such as atherosclerosis, asthma, lung and neurodegenerative diseases. Detection of nitrated proteins has been mainly based on the use of nitrotyrosine‐specific antibodies. In contrast, only a small number of nitration sites in proteins have been unequivocally identified by MS. We have used a monoclonal 3‐NT‐specific antibody, and have synthesized a series of tyrosine‐nitrated peptides of prostacyclin synthase (PCS) in which a single specific nitration site at Tyr‐430 had been previously identified upon reaction with peroxynitrite17. The determination of antibody‐binding affinity and specificity of PCS peptides nitrated at different tyrosine residues (Tyr‐430, Tyr‐421, Tyr‐83) and sequence mutations around the nitration sites provided the identification of an epitope motif containing positively charged amino acids (Lys and/or Arg) N‐terminal to the nitration site. The highest affinity to the anti‐3NT‐antibody was found for the PCS peptide comprising the Tyr‐430 nitration site with a KD of 60 nM determined for the peptide, PCS(424‐436‐Tyr‐430NO2); in contrast, PCS peptides nitrated at Tyr‐421 and Tyr‐83 had substantially lower affinity. ELISA, SAW bioaffinity, proteolytic digestion of antibody‐bound peptides and affinity‐MS analysis revealed highest affinity to the antibody for tyrosine‐nitrated peptides that contained positively charged amino acids in the N‐terminal sequence to the nitration site. Remarkably, similar N‐terminal sequences of tyrosine‐nitration sites have been recently identified in nitrated physiological proteins, such as eosinophil peroxidase and eosinophil‐cationic protein. Copyright © 2011 European Peptide Society and John Wiley & Sons, Ltd.
Analysis of the interacting surface of maurotoxin with the voltage‐gated Shaker B K+ channel
Authors: Fajloun Ziad , Andreotti Nicolas , Fathallah Mohamed , Sabatier Jean‐Marc , De Waard Michel 
Published: January 2011

AbstractMaurotoxin (MTX) is a 34‐residue toxin that was isolated initially from the venom of the scorpion Scorpio maurus palmatus. Unlike the other toxins of the α‐KTx6 family (Pi1, Pi4, Pi7, and HsTx1), MTX exhibits a unique disulfide bridge organization of the type C1C5, C2C6, C3C4, and C7C8 (instead of the conventional C1C5, C2C6, C3C7, and C4C8, herein referred to as Pi1‐like) that does not prevent its folding along the classic α/β scaffold of scorpion toxins. MTXPi1 is an MTX variant with a conventional pattern of disulfide bridging without any primary structure alteration of the toxin. Here, using MTX and/or MTXPi1 as models, we investigated how the type of folding influences toxin recognition of the Shaker B potassium channel. Amino acid residues of MTX that were studied for Shaker B recognition were selected on the basis of their homologous position in charybdotoxin, a three disulfide‐bridged scorpion toxin also active on this channel type. These residues favored either an MTX‐ or MTXPi1‐like folding. Our data indicate clearly that Lys23 and Tyr32 (two out of ten amino acid residues studied) are the most important residues for Shaker B channel blockage by MTX. For activity on SKCa channels, the same amino acid residues also affect, directly or indirectly, the recognition of SK channels. The molecular modeling technique and computed docking indicate the existence of a correlation between the half cystine pairings of the mutated analogs and their activity on the Shaker B K+ channel. Overall, mutations in MTX could, or could not, change the reorganization of disulfide bridges of this molecule without affecting its α/β scaffold. However, changing of the peptide backbone (cross linking disulfide bridges from MTX‐like type vs MTXPi1‐like type) appears to have less impact on the molecule activity than mutation of certain key amino acids such as Lys23 and Tyr32 in this toxin. Copyright © 2011 European Peptide Society and John Wiley & Sons, Ltd.
Single‐photon detection by Si single‐electron FETs
Authors: Tabe Michiharu , Udhiarto Arief , Moraru Daniel , Mizuno Takeshi 
Published: January 2011

AbstractWe have demonstrated that Si single‐electron SOI‐MOSFETs with multidots channel have attractive new functions such as single‐photon detection. Multidots formed by nanoscale selective oxidation of thin SOI layer have been used for photon detection. Most recently, we have investigated photon detection capabilities of FETs having phosphorus (P)‐doped channel. In such P‐doped FETs, each P donor works as a quantum dot for electrons and single‐electron transport is achieved by tunnelling through donor potentials. Using such P‐doped FETs, single‐photon detection has been demonstrated. Furthermore, in order to directly observe the spatial landscape of even a single‐dopant potential, we have developed low‐temperature‐Kelvin probe force microscopy (LT‐KFM) and succeeded in detecting a single‐dopant potential in the channel region. In this paper, we present results of photon‐induced random telegraph signals in crystalline‐dot‐type and donor‐dot‐type multidot single‐electron SOI‐MOSFETs, and direct observation of a single‐dopant potential by LT‐KFM.
Interdependency of surface morphology and wavelength fluctuations of indium‐rich InGaN/GaN quantum wells
Authors: Lermer Teresa , Pietzonka Ines , Avramescu Adrian , Brüderl Georg , Müller Jens , Lutgen Stephan , Strauss Uwe 
Published: January 2011

AbstractThe origin of indium fluctuations in indium‐rich quantum wells (QWs) is of high interest for direct green laser diodes. We present the correlation of morphological features such as macrosteps investigated by AFM measurements and wavelength fluctuations seen in cathodoluminescence images of InGaN/GaN QWs grown by metal organic vapor phase epitaxy. We observe an opposed wavelength shift of 5 nm in the vicinity of macrosteps for the investigated UV and green InGaN QW samples. We present a growth model taking adsorption, desorption, and migration processes into account to explain this difference via a temperature dependent change in indium incorporation in the vicinity of the observed macrostep edges.
A novel substrate LaBGeO5 lattice‐matched to InN
Authors: Miyazawa Shintaro , Ichikawa Satoru , Liu Yuhuai , Ji Shiyang , Matsuoka Takashi , Nakae Hideo 
Published: January 2011

AbstractAs a novel substrate with a lattice‐mismatch of only 0.8% along the a‐axis to InN thin films, we propose LaBGeO5 (LBGO) with a trigonal symmetry for the first time. A single crystal growth of LBGO by Czochralski (Cz)‐pulling was investigated, and c‐axis‐oriented LBGO single crystals with high crystallinity were successfully grown with a pulling rate of 0.5 mm/h in O2‐atomosphere. c‐axis oriented InN film was subsequently grown on the obtained LBGO {001} substrate by metalorganic vapor epitaxy (MOVPE), and it was verified that InN film on LBGO was less‐strained as compared with that on {001} sapphire substrate. It is stressed to say that LBGO can be expected as a lattice‐matching substrate for the epitaxial growth of InN.
Kelvin probe force microscopy in the presence of intrinsic local electric fields
Authors: Baumgart Christine , Müller Anne‐Dorothea , Müller Falk , Schmidt Heidemarie 
Published: January 2011

AbstractKelvin probe force microscopy (KPFM) is used to investigate the electrostatic force between a conductive probe and doped semiconductors. The observed frequency dependence of the probed KPFM bias is strongly related to sample‐specific intrinsic local electric fields. Equilibrium drift and diffusion of excess charge carriers at low operation frequencies influence the characteristics of the asymmetric electric dipole in the surface region of the investigated semiconductors during the KPFM measurement. The sample‐specific KPFM background signal does not influence the frequency‐dependent lateral variation of the electrical signal. The KPFM bias probed on doped semiconductor nanostructures with high or small enough operation frequencies allows for quantitative dopant profiling or investigation of diffusion processes in internal electric fields, respectively.