bluuebunny/crossref_metadata_2025_split huggingface dataset:
Die ersten Listen und Aufsammlungen von Pflanzen aus Alaska – Georg Wilhelm Stellers botanische Arbeiten in Amerika
Authors: Jäger E. J. 
Published: August 2010

AbstractThe first plant lists and collections from Alaska – Georg Wilhelm Steller's botanical work in America.Georg Wilhelm Steller was the first botanist in Alaska. As a member of the 2nd Kamchatka Expedition under Vitus Bering, he botanized for six hours on July 20, 1741 (resp. on July 31 after the modern calendar), on the western shore of Kayak Island and some hours on August 30–31 (resp. September 10–11), 1741, on Nagai Island, Alaska.The result of these short excursions were 3 manuscripts: the „Catalogus plantarum intra sex horas…”︁, the „Mantissa plantarum minus aut plane incognitarum”︁, and the „Catalogus seminum anno 1741 in America septemtrionali…”︁. (Steller's „Cat(alogus) Plant(arum) Amer(icae)”︁ which Gmelin had at hand when writing his „Flora sibirica”︁ is hitherto unknown.).While the first of these manuscripts was already published by Stejneger (1936) and analyzed by Thilenius (1992), the second and third are first published, translated and analysed here. (Copies of the manuscripts were kindly made available by Dr. W. Hintzsche and the Archive of the Russian Academy of Sciences.) Furthermore, a first translation and new interpretation is presented also for the „Catalogus plantarum”︁. By comparison with the two other manuscripts, modern literature and with the help of the old botanical literature that Steller used on his journey, most of Steller's plant names could be translated into modern botanical nomenclature. A recent plant collection from Kayak by U. Wannhoff, which is also published here, was extremely helpful.Whereas Thilenius concludes that less then 10 percent of Steller's 143 names in the „Catalogus plantarum”︁ are correct, the new analysis reveals that most of the names refer precisely to plants that grow
Ergänzungen zur Oberkreide‐Flora von Quedlinburg (Deutschland) und einiger weiterer Kreide‐Fundstätten (geographische Beziehungen)
Authors: Rüffle L. , Trostheide F. 
Published: August 2010

AbstractDie Santon‐Flora der Altenburg bei Quedlinburg enthält außer Gymnospermen auch zahlreiche Angiospermen‐Blätter, besonders von Platanaceae und Monimiaceae. Die Farne der Lokalität, ebenso wie die Gymnospermen, stammen teilweise bereits aus dem Jura.Die Angiospermen‐Arten gehen teilweise in das Alttertiär (Paläozän, Eozän) über. Einige von ihnen sind Relikte bis in die Gegenwart auf verschiedenen Kontinenten. Die beschriebenen Arten sind mehr oder weniger identisch mit denen der amerikanischen Oberkreide. In dem hier vorliegenden Material fanden sich keine Epidermisreste.
Three new species of Philodendron (Araceae) from the Ducke Forest Reserve, central Amazonas, Brazil
Authors: Soares M. L. , Mayo S. J. 
Published: August 2010

AbstractThree new species of Philodendron from the Reserva Florestal Ducke near Manaus, Brazil are described and illustrated: Philodendron toshibai, Ph. hopkinsianum and Ph. tortum.
LTQ‐iQuant: A freely available software pipeline for automated and accurate protein quantification of isobaric tagged peptide data from LTQ instruments
Authors: Onsongo Getiria , Stone Matthew D. , Van Riper Susan K. , Chilton John , Wu Baolin , Higgins LeeAnn , Lund Troy C. , Carlis John V. , Griffin Timothy J. 
Published: August 2010

AbstractPulsed Q dissociation enables combining LTQ ion trap instruments with isobaric peptide tagging. Unfortunately, this combination lacks a technique which accurately reports protein abundance ratios and is implemented in a freely available, flexible software pipeline. We developed and implemented a technique assigning collective reporter ion intensity‐based weights to each peptide abundance ratio and calculating a protein's weighted average abundance ratio and p‐value. Using an iTRAQ‐labeled standard mixture, we compared our technique's performance to the commercial software MASCOT, finding that it performed better than MASCOT's nonweighted averaging and median peptide ratio techniques, and equal to its weighted averaging technique. We also compared performance of the LTQ‐Orbitrap plus our technique to 4800 MALDI TOF/TOF plus Protein Pilot, by analyzing an iTRAQ‐labeled stem cell lysate. We found highly correlated protein abundance ratios, indicating that the LTQ‐Orbitrap plus our technique yields results comparable to the current standard. We implemented our technique in a freely available, automated software pipeline, called LTQ‐iQuant, which is mzXML‐compatible; supports iTRAQ 4‐plex and 8‐plex LTQ data; and can be modified for and have weights trained to a user's LTQ and other isobaric peptide tagging methods. LTQ‐iQuant should make LTQ instruments and isobaric peptide tagging accessible to more proteomic researchers.
Data‐directed top‐down Fourier‐transform mass spectrometry of a large integral membrane protein complex: Photosystem II from Galdieria sulphuraria
Authors: Thangaraj Balakumar , Ryan Christopher M. , Souda Puneet , Krause Kirsten , Faull Kym F. , Weber Andreas P. M. , Fromme Petra , Whitelegge Julian P. 
Published: August 2010

AbstractHigh‐resolution top‐down MS was used to characterize eleven integral and five peripheral subunits of the 750 kDa photosystem II complex from the eukaryotic red alga, Galdieria sulphuraria. The primary separation used LC MS with concomitant fraction collection (LC‐MS+), yielding around 40 intact mass tags at 100 ppm mass accuracy on a low‐resolution ESI mass spectrometer, whose retention and mass were used to guide subsequent high‐resolution top‐down nano‐electrospray FT ion‐cyclotron resonance MS experiments (FT‐MS). Both collisionally activated and electron capture dissociation were used to confirm the presence of eleven small subunits to mass accuracy within 5 ppm; PsbE, PsbF, PsbH, PsbI, PsbJ, PsbK, PsbL, PsbM, PsbT, PsbX and PsbZ. All subunits showed covalent modifications that fall into three classes including retention of initiating formyl‐methionine, removal of methionine at the N‐terminus with or without acetylation, and removal of a longer N‐terminal peptide. Peripheral subunits identified by top‐down analysis included oxygen‐evolving complex subunits PsbO, PsbU, PsbV, as well as Psb28 (PsbW) and Psb27 (“PsbZ‐like”). Top‐down high‐resolution MS provides the necessary precision, typically less than 5 ppm, for identification and characterization of polypeptide composition of these important membrane protein complexes.
A novel role of CCN3 in regulating endothelial inflammation
Authors: Lin Zhiyong , Natesan Viswanath , Shi Hong , Hamik Anne , Kawanami Daiji , Hao Caili , Mahabaleshwar Ganapati H. , Wang Weiye , Jin Zheng‐Gen , Atkins G. Brandon , Firth Sue M. , Rittié Laure , Perbal Bernard , Jain Mukesh K. 
Published: August 2010

AbstractThe vascular endothelium plays a fundamental role in the health and disease of the cardiovascular system. The molecular mechanisms regulating endothelial homeostasis, however, remain incompletely understood. CCN3, a member of the CCN (Cyr61, Ctgf, Nov) family of cell growth and differentiation regulators, has been shown to play an important role in numerous cell types. The function of CCN3 in endothelial cells has yet to be elucidated. Immunohistochemical analysis of CCN3 expression in mouse tissues revealed robust immunoreactivity in the endothelium of large arteries, small resistance vessels, and veins. We found that CCN3 expression in human umbilical vein endothelial cells (HUVECs) is transcriptionally induced by laminar shear stress (LSS) and HMG CoA‐reductase inhibitors (statins). Promoter analyses identified the transcription factor Kruppel‐like factor 2 (KLF2) as a direct regulator of CCN3 expression. In contrast to LSS, proinflammatory cytokines reduced CCN3 expression. Adenoviral overexpression of CCN3 in HUVEC markedly inhibited the cytokine‐mediated induction of vascular adhesion molecule‐1 (VCAM‐1). Consistent with this observation, CCN3 significantly reduced monocyte adhesion. Conversely, CCN3 knockdown in HUVECs resulted in enhancement of cytokine‐induced VCAM‐1 expression. Concordant effects were observed on monocyte adhesion. Gain and loss‐of‐function mechanistic studies demonstrated that CCN3 negatively regulates nuclear factor kappaB (NF‐κB) activity by reducing its translocation into the nucleus and subsequent binding to the VCAM‐1 promoter, suggesting that CCN3’s anti‐inflammatory effects occur secondary to inhibition of NF‐κB nuclear accumulation. This study identifies CCN3 as a novel regulator of endothelial proinflammatory activation.
Ovulation Induction in Polycystic Ovary Syndrome
Authors: Vause Tannys D.R. , Cheung Anthony P. , Sierra Sony , Claman Paul , Graham James , Guillemin Jo‐Anne , Lapensée Louise , Steward Sabrina , Vause Tannys D.R. , Wong Benjamin Chee‐Man 
Published: August 2010

AbstractObjectiveTo review current non‐pharmacologic and pharmacologic options for ovulation induction in women with polycystic ovary syndrome (PCOS).OptionsThis guideline reviews the evidence for the various options for ovulation induction in PCOS.OutcomesOvulation, pregnancy and live birth rates, risks, and side effects are the outcomes of interest.EvidencePublished literature was retrieved through searches of Medline using appropriate controlled vocabulary and key words. Results were restricted to systematic reviews, randomized control trials/controlled clinical trials, and observational studies. Grey (unpublished) literature was identified through searching the websites of health technology assessment and of health technology assessment‐related agencies, clinical practice guideline collections, clinical trial registries, and national and international medical specialty societies.ValuesThe evidence gathered was reviewed and evaluated by the Reproductive Endocrinology and Infertility Committee of the Society of Obstetricians and Gynaecologists of Canada. The quality of evidence was quantified using the Canadian Task Force on Preventive Health Care.Benefits, Harms, and CostsBenefits include weight reduction and improvements in ovulation, pregnancy, and live birth rates. Potential harms include medication side effects and multiple pregnancies.ValidationThese guidelines have been reviewed and approved by the Reproductive Endocrinology and Infertility Committee of the SOGC.SponsorThe Society of Obstetricians and Gynaecologists of Canada.
Tolerance and safety of the potentially probiotic strainLactobacillus rhamnosusPRSF-L477: a randomised, double-blind placebo-controlled trial in healthy volunteers
Authors: Wind Richèle D. , Tolboom Hermien , Klare Ingo , Huys Geert , Knol Jan 
Published: August 2010

In Europe, the speciesLactobacillus rhamnosusis currently on the Qualified Presumption of Safety list used by the European Food Safety Authority (EFSA) for internal safety assessment, but according to the EFSA the species should remain a topic of surveillance. In the present study, the safety and tolerance of the potentially probiotic strainL. rhamnosusPRSF-L477 was investigated in a placebo-controlled double-blind volunteer trial following FAO/WHO guidelines. A total of thirty-four subjects received daily doses of 1 × 1011colony-forming units (cfu) ofL. rhamnosusPRSF-L477 (n17) or placebo (n17) for a period of 3 weeks, followed by a wash-out period of another 3 weeks. A questionnaire on gastrointestinal tolerance and a diary was kept daily to record compliance throughout these 6 weeks. Faecal and blood samples were collected for microbiological and haematological analysis. The recorded gastrointestinal symptoms, defecation frequency and stool consistency were not influenced indicating thatL. rhamnosusPRSF-L477 was well tolerated. The speciesL. rhamnosuswas detected in the faeces of sixteen out of seventeen subjects of the probiotic group during the intervention period. Using pulsed-field gel electrophoresis, re-isolates ofL. rhamnosusPRSF-L477 were confirmed in nine of these subjects. Antibiotic susceptibility profiles of these re-isolates were unchanged compared with PRSF-L477. No clinically relevant changes in blood parameters such as liver and kidney function and no serious adverse events appeared during and after administration. Therefore, we conclude thatL. rhamnosusPRSF-L477 can safely be administrated to healthy subjects at a daily dose of 1 × 1011 cfu.
Risk factors for fluoroquinolone resistance in Enterococcus urinary tract infections in hospitalized patients
Authors: RATTANAUMPAWAN P. , TOLOMEO P. , BILKER W. B. , FISHMAN N. O. , LAUTENBACH E. 
Published: August 2010

SUMMARYPast studies exploring risk factors for fluoroquinolone (FQ) resistance in urinary tract infections (UTIs) focused only on UTIs caused by Gram-negative pathogens. The epidemiology of FQ resistance in enterococcal UTIs has not been studied. We conducted a case-control study at two medical centres within the University of Pennsylvania Health System in order to identify risk factors for FQ resistance in enterococcal UTIs. Subjects with positive urine cultures for enterococci and meeting CDC criteria for healthcare-acquired UTI were eligible. Cases were subjects with FQ-resistant enterococcal UTI. Controls were subjects with FQ-susceptible enterococcal UTI and were frequency matched to cases by month of isolation. A total of 136 cases and 139 controls were included from 1 January 2003 to 31 March 2005. Independent risk factors [adjusted OR (95% CI)] for FQ resistance included cardiovascular diseases [2·24 (1·05–4·79), P=0·037], hospitalization within the past 2 weeks [2·08 (1·05–4·11), P=0·035], hospitalization on a medicine service [2·15 (1·08–4·30), P<0·030], recent exposure to β-lactamase inhibitors (BLIs) [14·98 (2·92–76·99), P<0·001], extended spectrum cephalosporins [9·82 (3·37–28·60), P<0·001], FQs [5·36 (2·20–13·05), P<0·001] and clindamycin [13·90 (1·21–10·49), P=0·035]. Use of BLIs, extended spectrum cephalosporins, FQs and clindamycin was associated with FQ resistance in enterococcal uropathogens. Efforts to curb FQ resistance should focus on optimizing use of these agents.
Glucose promotion of GABA metabolism contributes to the stimulation of insulin secretion in β-cells
Authors: Pizarro-Delgado Javier , Braun Matthias , Hernández-Fisac Inés , Martín-Del-Río Rafael , Tamarit-Rodriguez Jorge 
Published: August 2010

We have demonstrated recently that branched-chain α-keto acid stimulation of insulin secretion is dependent on islet GABA (γ-aminobutyric acid) metabolism: GABA transamination to succinic semialdehyde is increased by 2-oxoglutarate, generated in α-keto acid transamination to its corresponding α-amino acid. The present work was aimed at investigating whether glucose also promotes islet GABA metabolism and whether the latter contributes to the stimulation of insulin secretion. Glucose (20 mM) decreased both the content and release of islet GABA. Gabaculine (1 mM), a GABA transaminase inhibitor, partially suppressed the secretory response of rat perifused islets to 20 mM glucose at different L-glutamine concentrations (0, 1 and 10 mM), as well as the glucose-induced decrease in islet GABA. The drug also reduced islet ATP content and the ATP/ADP ratio at 20 mM glucose. Exogenous succinic semialdehyde induced a dose-dependent increase in islet GABA content by reversal of GABA transamination and a biphasic insulin secretion in the absence of glucose. It depolarized isolated β-cells and triggered action potential firing, accompanied by a reduction of membrane currents through ATP-sensitive K+ channels. The gene expression and enzyme activity of GABA transaminase were severalfold higher than that of 2-oxoglutarate dehydrogenase in islet homogenates. We conclude that, at high glucose concentrations, there is an increased diversion of glucose metabolism from the citric acid cycle into the ‘GABA shunt’. Semialdehyde succinic acid is a cell-permeant ‘GABA-shunt’ metabolite that increases ATP and the ATP/ADP ratio, depolarizes β-cells and stimulates insulin secretion. In summary, an increased islet GABA metabolism may trigger insulin secretion.
Structural basis for high-affinity HER2 receptor binding by an engineered protein
Authors: Eigenbrot Charles , Ultsch Mark , Dubnovitsky Anatoly , Abrahmsén Lars , Härd Torleif 
Published: August 2010

The human epidermal growth factor receptor 2 (HER2) is specifically overexpressed in tumors of several cancers, including an aggressive form of breast cancer. It is therefore a target for both cancer diagnostics and therapy. The 58 amino acid residue Z
her
2 affibody molecule was previously engineered as a high-affinity binder of HER2. Here we determined the structure of Z
her
2 in solution and the crystal structure of Z
her
2 in complex with the HER2 extracellular domain. Z
her
2 binds to a conformational epitope on HER2 that is distant from those recognized by the therapeutic antibodies trastuzumab and pertuzumab. Its small size and lack of interference may provide Z
her
2 with advantages for diagnostic use or even for delivery of therapeutic agents to HER2-expressing tumors when trastuzumab or pertuzumab are already employed. Biophysical characterization shows that Z
her
2 is thermodynamically stable in the folded state yet undergoing conformational interconversion on a submillisecond time scale. The data suggest that it is the HER2-binding conformation that is formed transiently prior to binding. Still, binding is very strong with a dissociation constant
K

D

= 22 pM, and perfect conformational homogeneity is therefore not necessarily required in engineered binding proteins. A comparison of the original Z domain scaffold to free and bound Z
her
2 structures reveals how high-affinity binding has evolved during selection and affinity maturation and suggests how a compromise between binding surface optimization and stability and dynamics of the unbound state has been reached.
Scanning ultrafast electron microscopy
Authors: Yang Ding-Shyue , Mohammed Omar F. , Zewail Ahmed H. 
Published: August 2010

Progress has been made in the development of four-dimensional ultrafast electron microscopy, which enables space-time imaging of structural dynamics in the condensed phase. In ultrafast electron microscopy, the electrons are accelerated, typically to 200 keV, and the microscope operates in the transmission mode. Here, we report the development of scanning ultrafast electron microscopy using a field-emission-source configuration. Scanning of pulses is made in the single-electron mode, for which the pulse contains at most one or a few electrons, thus achieving imaging without the space-charge effect between electrons, and still in ten(s) of seconds. For imaging, the secondary electrons from surface structures are detected, as demonstrated here for material surfaces and biological specimens. By recording backscattered electrons, diffraction patterns from single crystals were also obtained. Scanning pulsed-electron microscopy with the acquired spatiotemporal resolutions, and its efficient heat-dissipation feature, is now poised to provide in situ 4D imaging and with environmental capability.
Gastric adenocarcinoma.
Authors: Correa P. , Piazuelo M. B. 
Published: August 2010

Abstract

This chapter reviews the risk factors and pathology of Helicobacter pylori-associated gastric adenocarcinoma. The possible reasons regarding the discrepancy between gastric cancer rates and H. pylori infection prevalence are discussed. The mechanisms of H. pylori-related gastric carcinogenesis and cancer control are described.
Host genetic factors in susceptibility and resistance to Helicobacter pylori pathogenesis.
Authors: Sutton P. , Every A. L. , Harbour S. N. 
Published: August 2010

Abstract

This chapter discusses the association between host variability in immune-related genes (cytokines, pathogen recognition receptors, mucins) and susceptibility to Helicobacter pylori-associated pathologies (peptic ulcer, gastritis and gastric cancer). The effect of gender on H. pylori infection and its pathological consequences are described.
Lipopolysaccharides of Helicobacter pylori: importance in gastric adaptation and pathogenesis.
Authors: Moran A. P. 
Published: August 2010

Abstract

This chapter discusses how the structure and properties of Helicobacter pylori lipopolysaccharides (LPS) are adapted and modelled to fit the environmental niche of this bacterium and the pathogenic consequences of these structures, focusing on the lipid A moiety and O-antigen of this molecule. The low immunological activity of H. pylori LPS, the fine structure and biosynthesis of H. pylori lipid A, and the relevance of low LPS and lipid A immunoactivities to H. pylori pathogenesis are described. The role of H. pylori-expressed Lewis antigens in gastric adaptation and modulation of the inflammatory response is discussed.
The importance of predator–prey overlap: predicting North Sea cod recovery with a multispecies assessment model
Authors: Kempf Alexander , Dingsør Gjert Endre , Huse Geir , Vinther Morten , Floeter Jens , Temming Axel 
Published: August 2010

Abstract
Kempf, A., Dingsør, G. E., Huse, G., Vinther, M., Floeter, J., and Temming, A. 2010. The importance of predator–prey overlap: predicting North Sea cod recovery with a multispecies assessment model. – ICES Journal of Marine Science, 67: 1989–1997. The overlap between predator and prey is known as a sensitive parameter in multispecies assessment models for fish, and its parameterization is notoriously difficult. Overlap indices were derived from trawl surveys and used to parametrize the North Sea stochastic multispecies model. The effect of time-invariant and year- and quarter-specific overlap estimates on the historical (1991–2007) and predicted trophic interactions, as well as the development of predator and prey stocks, was investigated. The focus was set on a general comparison between single-species and multispecies forecasts and the sensitivity of the predicted development of North Sea cod for the two types of overlap implementation. The spatial–temporal overlap between cod and its predators increased with increasing temperature, indicating that foodweb processes might reduce the recovery potential of cod during warm periods. Multispecies scenarios were highly influenced by assumptions on future spatial overlap, but they predicted a considerably lower recovery potential than single-species predictions did. In addition, a recovery of North Sea cod had strong negative effects on its prey stocks. The consequences of these findings for management are discussed.