bluuebunny/crossref_metadata_2025_split huggingface dataset:
Human rights among Korean dental hygiene students in hospital clinical practice
Authors: Won Yoon-Ah , Noh Hie-Jin 
Published: June 2024

Abstract
Background
The human rights of dental hygiene students should be guaranteed during practice at medical institutions for their mental and physical health as well as professionalism, for patient safety. Safe and well guaranteed clinicians can perform their work in a more stable way. This study investigated the human rights circumstances of dental hygiene students during their hospital clinical practice at dental institutions.

Methods
This study used a cross-sectional survey design. Convenience sampling was conducted on 121 third- and fourth-year dental hygiene students from universities in Seoul, Gyeonggi, and Gangwon. The survey used the Human Rights Indicators for Dental Hygiene Students to investigate the rights to safety, equality, and personality to understand participants’ experiences of guaranteed fundamental rights. Data were collected from October 31 to November 8, 2019. A chi-square test was used to assess differences in experience according to general characteristics.

Results
During dental hygiene practice at dental institutions, less than 50% of students felt safe. When human rights violations occurred in dental institutions, only 42.4% of students received guidance on response measures from their universities. While 72.1% of students who practiced at dental university hospitals were given information on first aid supplies (facilities) within dental institutions, only approximately 45% of students who practiced at lower-scale dental institutions were given this information (p < 0.05). Regarding equality rights, only 52.5% of trainees reported that they had received equal treatment from healthcare workers during hospital clinical practice.

Conclusions
During dental practice at dental institutions, Korean dental hygiene students confirmed that human rights (including safety rights, equality rights, and personality rights) were guaranteed to varying degrees. Dental hygiene students’ rights during hospital clinical practice in dental institutions should be guaranteed across institutions regardless of their scale. This is necessary for dental hygiene students’ human rights and safe policies and guidelines in dental institution clinical practice and regular monitoring systems.
Psychometric evaluation of the Vietnamese version of nurses’ ethical behaviors for protecting patient rights scale (V-NEBPPRS): a methodological study
Authors: Do Thi Ninh , Lee Gunjeong , Susmarini Dian 
Published: June 2024

Abstract
Background
Recognizing patients’ rights as fundamental human rights, the global healthcare community, including the World Health Organization and various nursing organizations, has emphasized the critical role of nurses in upholding these rights through ethical practice and patient-centered care. However, in the complex landscape of healthcare, nurses in Vietnam face various ethical issues and challenges that may impede their ability to protect patient rights effectively, necessitating tools for better ethical decision-making and practice.

Purpose
This study aims to translate the Nurses’ Ethical Behaviours for Protecting Patient Rights Scale (NEBPPR) into Vietnamese and evaluate the validity and reliability of the V-NEBPPRS.

Methods
The original scale underwent a cross-cultural translation process to be adapted into Vietnamese. Construct validity was assessed using confirmatory factor analysis (CFA). The convergent validity, discriminant validity, and reliability of the V-NEBPPRS were evaluated.

Results
After removing four items with factor loading below 0.5, the V-NEBPPRS comprises 24 items divided into five factors. CFA demonstrated a good model fit (χ2/df = 2.86; GFI = 0.87; IFI = 0.85; CFI = 0.84; RMSEA = 0.07). Convergent and discriminant validity were confirmed with extracted mean variance ranging from 0.54 to 0.67, 0.54 to 0.67, and composite reliability from 0.73 to 0.81. Cronbach’s α coefficient was 0.85 for the total scale and ranged from 0.70 to 0.79 for five subscales.

Conclusion
The V-NEBPPRS is a reliable tool, providing nursing leaders and researchers with the means to utilize the V-NEBPPRS for assessing and promoting nurses’ awareness and behaviour in safeguarding patients’ rights, thereby contributing to improved overall health outcomes.
Healthcare professionals’ experiences of video consultations in palliative care in rural areas: an intervention study in community care
Authors: Brännström Margareta , Philipsson Johan , Andersson Sofia 
Published: June 2024

Abstract
Background
The population is aging, leading to an increased need for palliative care and end-of-life care. There is a lack of research on the use of video consultations for knowledge transfer between specialist and general palliative care. The aim of this study was to describe healthcare professionals’ experiences of video consultations in palliative care in community homecare and nursing homes in rural areas.

Methods
Individual interviews (n = 11) were conducted with five community nurses, one occupational therapist, two specialist palliative nurses, and three specialist palliative care physicians. The data were analysed using reflexive thematic analysis.

Results
The analysis identified three themes: feeling comfortable with increased availability of specialist expertise; seeing each other facilitates communication; and being supported by physically present care professionals is essential.

Conclusion
HCPs suggest that video consultations are an effective way to increase access to specialist palliative care and provide more equal care to patients with palliative care needs in rural community care.
Multi-omics profiling reveal cells with novel oncogenic cluster, TRAP1low/CAMSAP3low, emerge more aggressive behavior and poor-prognosis in early-stage endometrial cancer
Authors: Mao Xiaodan , Tang Xiaoyue , Ye Jingxuan , Xu Shuxia , Wang Yue , Liu Xianhua , Wu Qibin , Lin Xite , Zhang Maotong , Liu Jiangfeng , Yang Juntao , Sun Pengming 
Published: June 2024

AbstractThe clinical heterogeneity of early-stage endometrial cancer (EC) is worthy of further study to identify high-quality prognostic markers and their potential role in aggressive tumor behavior. Mutation of TP53 was considered as an important primary triage in modified molecular typing for EC, it still cannot precisely predict the prognosis of EC. After proteomic analysis of cancer and para-cancerous tissues from 24 early-stage endometrioid EC patients with different survival outcomes, 13 differentially expressed proteins were screen out while 2 proteins enriched in p53 signaling pathway were further identified by single-cell transcriptome (scRNA-seq). Interestingly, tumor necrosis factor type-1 receptor-associated protein (TRAP1) and calmodulin-regulated spectrin-associated protein family member 3 (CAMSAP3) were found to be significantly downregulated in the specific cell cluster. Expectedly, the signature genes of TRAP1low/CAMSAP3low cluster included classical oncogenes. Moreover, close cellular interactions were observed between myeloid cells and the TRAP1low/CAMSAP3low cluster after systematically elucidating their relationship with tumor microenvironment (TME). The expression of TRAP1 and CAMSAP3 was verified by immunohistochemistry. Thus, a novel prediction model combining TRAP1, CAMSAP3 and TP53 was construct by multi-omics. Compared with the area under the curve, it demonstrated a significantly improvemrnt in the diagnostic efficacy in EC patients from TCGA bank. In conclusion, this work improved the current knowledge regarding the prognosis of early-stage EC through proteomics and scRNA-seq. These findings may lead to improvements in precise risk stratification of early-stage EC patients.
“In Sweden you are worthless. In Denmark you get an identity again” – on being perceived and received as a person who uses drugs in different drug policy settings
Authors: Holeksa Julie 
Published: June 2024

Abstract
Background
Policies to address substance use differ greatly between settings, where goals may range from zero-tolerance to harm reduction. Different approaches impact formats of care, policing, and even interpersonal interactions, and may play a role in the labelling and stigmatization of people who use drugs (PWUD). Where Sweden has a more restrictive policy, aiming to have a society free from drugs, Denmark has embraced harm reduction principles. The aim of this study was to explore PWUDs’ experiences of interpersonal interactions, policing, and service formats in the two countries.

Methods
The data consists of 17 qualitative semi-structured interviews with Swedish PWUD who have been in both Sweden and Denmark. Recruitment took place at harm reduction sites in both countries, and through snowball sampling.

Results
Participants reflected on how they were perceived by those in public spaces, and received by care systems and personnel. In public settings in Sweden, participants felt they were ignored, rendered invisible, and lost their humanity. In Denmark, they were perceived and acknowledged, valued as people. This was simultaneously linked to being embodied by the availability of differing service offerings and policing practices, which solidified their “right to be out” in public. Reflecting on their reception in the treatment system, strict formatting in Sweden caused participants to feel that an identity was projected upon them, limiting their opportunities or growth of new facets of identity. Care relations in Denmark fostered more opportunity for autonomy and trust.

Conclusion
A zero-tolerance policy and associated public discourses could solidify and universalize stigmatizing categorizations as a central feature of PWUD identity and reception from those around them, exacerbating social exclusion. Conversely, harm reduction-centered policies fostered positive interactions between individuals with care providers, public, and police, which may promote inclusion, empowerment, and wellbeing.
DNA methylation variations underlie lettuce domestication and divergence
Authors: Cao Shuai , Sawettalake Nunchanoke , Li Ping , Fan Sheng , Shen Lisha 
Published: June 2024

AbstractBackgroundLettuce (Lactuca sativa L.) is an economically important vegetable crop worldwide. Lettuce is believed to be domesticated from a single wild ancestorLactuca serriolaand subsequently diverged into two major morphologically distinct vegetable types: leafy lettuce and stem lettuce. However, the role of epigenetic variation in lettuce domestication and divergence remains largely unknown.ResultsTo understand the genetic and epigenetic basis underlying lettuce domestication and divergence, we generate single-base resolution DNA methylomes from 52Lactucaaccessions, including major lettuce cultivars and wild relatives. We find a significant increase of DNA methylation during lettuce domestication and uncover abundant epigenetic variations associated with lettuce domestication and divergence. Interestingly, DNA methylation variations specifically associated with leafy and stem lettuce are related to regulation and metabolic processes, respectively, while those associated with both types are enriched in stress responses. Moreover, we reveal that domestication-induced DNA methylation changes could influence expression levels of nearby and distal genes possibly through affecting chromatin accessibility and chromatin loop.ConclusionOur study provides population epigenomic insights into crop domestication and divergence and valuable resources for further domestication for diversity and epigenetic breeding to boost crop improvement.
Defatting of donor transplant livers during normothermic perfusion—a randomised clinical trial: study protocol for the DeFat study
Authors: Abbas Syed Hussain , Ceresa Carlo D. L. , Hodson Leanne , Nasralla David , Watson Christopher J. E. , Mergental Hynek , Coussios Constantin , Kaloyirou Fotini , Brusby Kerrie , Mora Ana , Thomas Helen , Kounali Daphne , Keen Katie , Pollok Joerg-Matthias , Gaurav Rohit , Iype Satheesh , Jassem Wayel , Perera M. Thamara PR , Hakeem Abdul Rahman , Knight Simon , Friend Peter J. 
Published: June 2024

Abstract
Background
Liver disease is the third leading cause of premature death in the UK. Transplantation is the only successful treatment for end-stage liver disease but is limited by a shortage of suitable donor organs. As a result, up to 20% of patients on liver transplant waiting lists die before receiving a transplant. A third of donated livers are not suitable for transplant, often due to steatosis. Hepatic steatosis, which affects 33% of the UK population, is strongly associated with obesity, an increasing problem in the potential donor pool. We have recently tested defatting interventions during normothermic machine perfusion (NMP) in discarded steatotic human livers that were not transplanted. A combination of therapies including forskolin (NKH477) and L-carnitine to defat liver cells and lipoprotein apheresis filtration were investigated. These interventions resulted in functional improvement during perfusion and reduced the intrahepatocellular triglyceride (IHTG) content. We hypothesise that defatting during NMP will allow more steatotic livers to be transplanted with improved outcomes.

Methods
In the proposed multi-centre clinical trial, we will randomly assign 60 livers from donors with a high-risk of hepatic steatosis to either NMP alone or NMP with defatting interventions. We aim to test the safety and feasibility of the defatting intervention and will explore efficacy by comparing ex-situ and post-reperfusion liver function between the groups. The primary endpoint will be the proportion of livers that achieve predefined functional criteria during perfusion which indicate potential suitability for transplantation. These criteria reflect hepatic metabolism and injury and include lactate clearance, perfusate pH, glucose metabolism, bile composition, vascular flows and transaminase levels. Clinical secondary endpoints will include proportion of livers transplanted in the two arms, graft function; cell-free DNA (cfDNA) at follow-up visits; patient and graft survival; hospital and ITU stay; evidence of ischemia-reperfusion injury (IRI); non-anastomotic biliary strictures and recurrence of steatosis (determined on MRI at 6 months).

Discussion
This study explores ex-situ pharmacological optimisation of steatotic donor livers during NMP. If the intervention proves effective, it will allow the safe transplantation of livers that are currently very likely to be discarded, thereby reducing waiting list deaths.

Trial registration
ISRCTN ISRCTN14957538. Registered in October 2022.
CTR > 0.7 predicts the subgroup of lung adenocarcinomas ≤ 2 cm at risk of poor outcome treated by sublobar resection compared to lobar resection
Authors: Jing Weiwei , Li Qi , Liu Mengxi , Zhang Yi , Chen Sifan , Zhang Ke , Li Dan , Zhao Min , Zheng Yineng , Li Wangjia , Wu Yangying , Xu Hongbo , Zhao Ziya , Kang Shaolei , Lv Fajin 
Published: June 2024

Abstract
Background
A standard surgical procedure for patients with small early-stage lung adenocarcinomas remains unknown. Hence, we aim in this study to assess the clinical utility of the consolidation-to-tumor ratio (CTR) when treating patients with small (2 cm) early stage lung cancers.

Methods
A retrospective cohort of 298 sublobar resection and 266 lobar resection recipients for early stage lung adenocarcinoma ≤ 2 cm was assembled from the First Affiliated Hospital of Chongqing Medical University between 2016 and 2019. To compare survival rates among the different groups, Kaplan-Meier curves were calculated, and the log-rank test was used. A multivariate Cox proportional hazard model was constructed utilizing variables that were significant in univariate analysis of survival.

Results
In the study, 564 patients were included, with 298 patients (52.8%) undergoing sublobar resection and 266 patients (47.2%) undergoing lobar resection. Regarding survival results, there was no significant difference in the 5-year overall survival (OS, P = 0.674) and 5-year recurrence-free survival (RFS, P = 0.253) between the two groups. Cox regression analyses showed that CTR ≥ 0.75(P < 0.001), age > 56 years (P = 0.007), and sublobar resection(P = 0.001) could predict worse survival. After examining survival results based on CTR categorization, we segmented the individuals into three categories: CTR<0.7, 0.7 ≤ CTR<1, and CTR = 1.The lobar resection groups had more favorable clinical outcomes than the sublobar resection groups in both the 0.7 ≤ CTR < 1(RFS: P < 0.001, OS: P = 0.001) and CTR = 1(RFS: P = 0.001, OS: P = 0.125). However, for patients with 0 ≤ CTR < 0.7, no difference in either RFS or OS was found between the lobar resection and sublobar resection groups, all of which had no positive events. Patients with a CTR between 0.7 and 1 who underwent lobar resection had similar 5-year RFS and OS rates compared to those with a CTR between 0 and 0.7 who underwent sublobar resection (100% vs. 100%). Nevertheless, a CTR of 1 following lobar resection resulted in notably reduced RFS and OS when compared to a CTR between 0.7 and 1 following lobar resection (P = 0.005 and P = 0.016, respectively).

Conclusion
Lobar resection is associated with better long-term survival outcomes than sublobar resection for small lung adenocarcinomas ≤ 2 cm and CTR ≥ 0.7.
Residual deficits of knee and hip joint coordination and clinical performance after return to sports in athletes with anterior cruciate ligament reconstruction
Authors: Sinsurin Komsak , Kiratisin Pongthanayos , Irawan Dimas Sondang , Vachalathiti Roongtiwa , Richards Jim 
Published: June 2024

Abstract
Background
Biomechanical changes and neuromuscular adaptations have been suggested as risk factors of secondary injury in individuals after anterior cruciate ligament reconstruction (ACLr). To achieve a better understanding of preventive mechanisms, movement quality is an important factor of consideration. Few studies have explored time-series analysis during landing alongside clinical performance in injured and non-injured individuals. The purpose of the study was to investigate the biomechanical risks of recurrent injury by comparing clinical and jump-landing performance assessments between athletes with ACLr and healthy controls.

Method
This study was observational study. Sixteen athletes with and without ACLr voluntarily participated in clinical and laboratory measurements. Single-leg hop distance, isokinetic tests, landing error score, and limb symmetry index (LSI) were included in clinical report. Lower limb movements were recorded to measure joint biomechanics during multi-directional landings in motion analysis laboratory. Hip-knee angle and angular velocity were explored using discrete time-point analysis, and a two-way mixed analysis of variance (2 × 4, group × jump-landing direction) was used for statistical analysis. Time series and hip-knee coordination analyses were performed using statistical parametric mapping and descriptive techniques.

Results
Significantly lower single-leg hop distance was noted in ACLr group (158.10 cm) compared to control group (178.38 cm). Although the hip and knee moments showed significant differences between four directions (p < 0.01), no group effect was observed (p > 0.05). Statistical parametric mapping showed significant differences (p ≤ 0.05) between groups for hip abduction and coordinate plot of hip and knee joints. Athletes with ACLr demonstrated a higher velocity of hip adduction. Time-series analysis revealed differences in coordination between groups for frontal hip and knee motion.

Conclusions
Athletes with ACLr landed with poor hip adduction control and stiffer knee on the involved side. Multi-directions landing should be considered over the entire time series, which may facilitate improved movement quality and return to sports in athletes with ACLr.
The anti-tumor immune response of radiotherapy combined with anti-CD200R1 in hepatocellular carcinoma.
Authors: Li Siqi , Li Kun , Jiang Peng , Li Hua , Yang Yang , Ye Linsen 
Published: June 2024

2525 Background: Various clinical trials demonstrate the efficacy of regional radiotherapy or chemotherapy in augmenting the therapeutic response to PD1/L1 antibody in hepatocellular carcinoma (HCC). However, a significant proportion of HCC patients still exhibit suboptimal responses to these combination therapies. The immune-suppressive tumor microenvironment (TME) poses a formidable barrier to effective immunotherapy. Recent evidences underscore the pivotal role played by myeloid cells in modulating the immunosuppressive TME following regional treatments. Therefore, targeting the myeloid immunosuppressive checkpoint represents a promising strategy for enhancing the efficacy of current local-immune combination therapy. Methods: Initially, single cell RNA sequencing (scRNA-seq) was conducted on tumor infilitrating immune cells (TIICs) of mouse HCC tissues three days post-radiotherapy. Subsequently, the phenotype of tumor-infiltrating CD200+ macrophages was characterized in mouse HCC tissues using flow cytometry. Next, the specific mechanism underlying CD200 up-regulation in tumor-infiltrating macrophages was investigated using wild-type mice and STING KO mice. Furthermore, the chemotactic and interactive mechanisms between CD200+ macrophages and CD200R+ myeloid-derived suppressor cells (MDSCs) were investigated through in vitro and in vivo experiments. Finally, the systemic antitumor effect of combined radiotherapy and anti-CD200R treatment was observed in multiple non-immunogenic HCC mouse models. Results: The infiltration level of CD200+ macrophages in HCC tissues significantly increase following local radiotherapy. The accumulation of CD200+ macrophage subset relies on potent STING activation subsequent to radiotherapy. In vitro and in vivo experiments demonstrate the robust capacity of CD200+ macrophages in enhancing myeloid immunosuppression. Mechanistically, CD200+ macrophages recruit CXCR2+ CD200R+ MDSC through secretion of CXCL1/3, thereby amplifying the proliferation and immunosuppressive capacity of this specific MDSC subset via interaction between CD200 and CD200R. Targeted blockade of CD200R improve the inhibitory immune microenvironment by suppressing the immunosuppressive capacity of myeloid suppressor cells. Lastly, combined anti-CD200R1 with radiotherapy sensitized the therapeutic effect of PD-1/L1 antibody in multiple non-immunogenic HCC mouse models. Conclusions: Our studies identify CD200+ macrophages as a crucial immune suppressor that is essential for tumor immune resistance following radiotherapy. These findings unveil a novel mechanism of immune evasion mediated by STING activation, thereby offering a potential target for further optimization of local-immune combination therapy in HCC.