bluuebunny/crossref_metadata_2025_split huggingface dataset:
Remote sensing image dehazing using generative adversarial network with texture and color space enhancement
Authors: Shen Helin , Zhong Tie , Jia Yanfei , Wu Chunming 
Published: May 2024

AbstractRemote sensing is gradually playing an important role in the detection of ground information. However, the quality of remote-sensing images has always suffered from unexpected natural conditions, such as intense haze phenomenon. Recently, convolutional neural networks (CNNs) have been applied to deal with dehazing problems, and some important findings have been obtained. Unfortunately, the performance of these classical CNN-based methods still needs further enhancement owing to their limited feature extraction capability. As a critical branch of CNNs, the generative adversarial network (GAN), composed of a generator and discriminator, has become a hot research topic and is considered a feasible approach to solving the dehazing problems. In this study, a novel dehazed generative adversarial network (GAN) is proposed to reconstruct the clean images from the hazy ones. For the generator network of the proposed GAN, the color and luminance feature extraction module and the high-frequency feature extraction module aim to extract multi-scale features and color space characteristics, which help the network to acquire texture, color, and luminance information. Meanwhile, a color loss function based on hue saturation value (HSV) is also proposed to enhance the performance in color recovery. For the discriminator network, a parallel structure is designed to enhance the extraction of texture and background information. Synthetic and real hazy images are used to check the performance of the proposed method. The experimental results demonstrate that the performance can significantly improve the image quality with a significant increment in peak-signal-to-noise ratio (PSNR). Compared with other popular methods, the dehazing results of the proposed method closely resemble haze-free images.
A dual mediation model of the association between principal inclusive leadership and teacher innovative behavior
Authors: Bao Yuangen 
Published: May 2024

AbstractTeacher innovation is crucial for the education system to adapt to contemporary society's evolving demands. However, the underlying mechanism of how inclusive leadership positively impacts employees’ innovative behavior remains incomplete. Therefore, drawing upon the social cognitive theory, this study aims to comprehensively examine the mediating effects of teachers’ psychological empowerment and school innovation climate on the relationship between inclusive leadership and innovative behavior among primary and junior teachers. A total of 358 valid questionnaires were administered to primary and junior teachers in mainland China using a convenience sampling approach. The data were analyzed using hierarchical linear modeling and bootstrap testing, revealing a positive relationship between principal inclusive leadership and teacher innovative behavior. Additionally, teachers’ psychological empowerment and school innovation climate were found to mediate this relationship, with the former playing a crucial role. The findings can enhance existing research on the impact of inclusive leadership in basic education. They also offer a new perspective for analyzing how inclusive leadership affects employees’ innovative behavior and provide valuable insights into stimulating innovation among primary and junior teachers.
Prognostic impact of FAN score in patients receiving nivolumab plus ipilimumab for metastatic renal cell carcinoma
Authors: Yamashita Shimpei , Hamamoto Shuzo , Furukawa Junya , Fujita Kazutoshi , Takahashi Masayuki , Miyake Makito , Ito Noriyuki , Iwamoto Hideto , Kohjimoto Yasuo , Hara Isao 
Published: May 2024

AbstractFAN score is reportedly associated with prognostic outcomes in patients with urothelial carcinoma being treated with immune check point inhibitors. However, the prognostic impact of pre-treatment FAN score in patients with metastatic renal cell carcinoma (RCC) treated with nivolumab plus ipilimumab remains unclear. We retrospectively evaluated the association between pre-treatment FAN score and prognostic outcomes in 154 patients with metastatic RCC treated with nivolumab plus ipilimumab. The pre-treatment FAN score was ‘0’ in 56 patients (36%), ‘1’ in 60 patients (40%), ‘2’ in 37 patients (24%) and ‘3’ in one patient (1%). Progression-free survival was not significantly different between patients with different FAN scores, but second progression-free survival (PFS2), cancer-specific survival (CSS) and overall survival (OS) were significantly different. In multivariable Cox proportional hazard analyses, FAN score ≥ 2 was a significant predictor of poor PFS2 (vs. FAN score 0, HR: 2.43, 95% CI 1.21–4.87, P = 0.01), poor CSS (vs. FAN score 0, HR: 2.71, 95% CI 1.13–6.47, P = 0.02) and poor OS (vs. FAN score 0, HR: 2.42, 95% CI 1.11–5.25, P = 0.02). High pre-treatment FAN score could be a significant independent predictor of poor prognosis in patients receiving nivolumab plus ipilimumab for metastatic RCC.
Ethnic diversity and divergent perceptions of climate change: a case study in Southwest China
Authors: Yang Hua , He Jun , Li Zhinong , Su Yufang , Xu Jianchu 
Published: May 2024

AbstractUnderstanding divergent perceptions of ethnic groups to climate change in mountainous regions home to multi-ethnic cultures and the factors influencing these perceptions is crucial for policymakers to predict the trending impacts of climate change and make long-term decisions. Based on the case of Southwest China, 1216 households were interviewed by questionnaire surveys to gain insight into the perceptions of local people on the dynamic evolution characteristics of climate events in the uplands of Yunnan, China, which is an area home to rich ethnic diversity, and also to determine the factors that influence these perceptions. Results indicated that climate events have now become important events for farmers’ livelihoods, ranking only after family diseases and livestock diseases. Drought, long-term drought, and erratic rainfall are the three kinds of climatic events with the most significant increase in frequency and severity in mountainous areas. Farmers’ perceptions on whether drought, long-term drought, and erratic rainfall occurred 10 years ago as well as changes in frequency and severity are significantly influenced by characteristics of respondents, ethnic culture, geographical environment of farmer residences, farmland characteristics, and sources of livelihood. Ultimately, taking ethnic differences into consideration for long-term planning will be an important part of the local response to climate change in the future.
Tourism as a “Tecnologia Social”: The Case of Ururay Group
Authors: Freitas João A. , Duarte Maurício Dias 
Published: May 2024

Summary
The notion of social technology introduces a novel paradigm for technological advancement that emphasizes inclusivity, participation, and democracy. It is rooted in the idea of local citizens as empowered agents. Within this framework, we spotlight the initiatives of Grupo Ururay, a collective based in the East Zone of São Paulo. Through cultural, touristic, and educational endeavors, they have illuminated the pivotal role played by Black and Indigenous communities in shaping the city’s history. The crux of our argument lies in portraying the collective’s utilization of tourism as a social technology, effectively employing it as a catalyst for societal transformation. To substantiate this claim, we will delineate some of the group’s initiatives and delve into the significance of revitalizing and celebrating counter-hegemonic histories within São Paulo’s urban landscape.

Information
© The Authors 2024
Buenos Aires Youth Tourism Access Policy
Authors: Tapia Silvia A. , Catalano Bárbara , Xaus Maria 
Published: May 2024

Summary
In Argentina, in the south of Latin America, student tourism has been associated with trips for young people who finish secondary school level. Such trips have a mountain and snow destination during the winter season and are organised privately between travel agencies and student groups, without the intervention of schools or state aid. Although sustained for decades, given their high costs, access to this type of tourism has become increasingly associated with upper-middle socio-economic sectors. During the COVID-19 pandemic, in 2021, the government of Buenos Aires, one of the largest and most populous provinces of Argentina, established the programme “End of Term Trip”, a local public policy of student tourism aimed at secondary school students of that province. Through a free trip package, it links the aims of universality and equality with a tourism policy that promotes new destinations, favours the work of travel agencies, and boosts off-season tourism. Based on documented data and an interview with a programme official this case study presents a tourism policy that seeks to boost the tourism sector and, at the same time, encourages the participation of young people from different social sectors in recreational tourism practices and their access to leisure and tourism rights.

Information
© The Authors 2024
Stage specific effects of insulin and Wnt on cell fate decisions in human induced pluripotent stem cell-derived cardiomyocytes
Authors: Yuan Q , Dinani R , Kuster D W D , Boon R A , Van Der Velden J , Buikema J W 
Published: May 2024

Abstract

Funding Acknowledgements
Type of funding sources: Public grant(s) – National budget only. Main funding source(s): Dutch Heart Foundation

Background
Reactivation of the so-called fetal gene programs and pathways have been reported in failing hearts, however, it remains unclear whether this phenomenon is beneficial or detrimental to the remaining functional myocardium. During cardiac development, the orchestration of these embryonic growth pathways, facilitate temporal and stage-specific cell fate decisions such as required for specification and growth of the various myocardial compartments. Previously, we demonstrated that molecular activation of the Wnt pathway resulted in massive expansion of immature human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs). Yet, it is unknown what exact cues guide the cell fate decisions of immature hiPSC-CMs to either proliferate or terminally differentiate into more mature cardiomyocytes.

Purpose
This work uncovers the embryonic growth pathways that control the cues for proliferation versus terminal differentiation in functional immature hiPSC-CMs.

Methods
We utilised spontaneously beating hiPSC-CMs at day 11 of differentiation and performed a combinatory screen for various dosages of CHIR99021/Wnt and Insulin/Akt pathway. Proliferation was determined by quantification of cell number and the proportion of Ki67 positive hiPSC-CMs. Terminal differentiation was evaluated by presence of more mature sarcomere markers and cell cycle exit.

Results
Our combinatory screen for Insulin/Akt and CHIR99021/Wnt demonstrates synergistic effects on proliferation of immature hiPSC-CMs, whereas the absence of these pathway activators leads to rapid cell cycle exit and terminal differentiation of hiPSC-CMs. Detailed characterization reveals that CHIR99021/Wnt also importantly regulates sarcomere homeostasis and architecture in hiPSC-CMs. Moreover, we identify a novel temporal interplay between CHIR99021/Wnt via TCF and Insulin/Akt via FOXO as regulators of cell-fate decision in immature hiPSC-CMs.

Conclusions
Molecular targeting of the embryonic growth pathways in acute and chronic forms of heart failure requires a detailed understanding of the specific effects on cardiomyocyte function and sarcomere architecture. This work provides a starting point to develop future strategies to target acute and chronic forms of heart failure via modulation of embryonic growth pathways.Graphical Summary
Phosphoinositide 3-Kinase C2alpha controls cardiac contractility through regulation of beta2-adrenergic receptor recycling
Authors: Cnudde S , Brand T , Fender J , Prever L , Murabito A , Russo M , Gulluni F , Lorenz K , Hirsch E , Ghigo A 
Published: May 2024

Abstract

Funding Acknowledgements
Type of funding sources: Public grant(s) – National budget only. Main funding source(s): Doctoral fellowship

Introduction
Phosphoinositide 3-kinase C2α (PI3KC2α) is a ubiquitously expressed class II PI3K isoform, which has been previously shown to be involved in the control of vesicular trafficking. However, its role in the myocardium has not been investigated. The primary aim of this study is to elucidate the mechanism by which mechanism PI3KC2α controls cardiac pathophysiology.

Methods
Cardiomyocyte-specific PI3KC2α knockout (PI3KC2α KO) animals were generated by crossing mice expressing a tamoxifen-inducible Cre recombinase under the control of the αMHC promoter with PI3KC2αflox/flox mice. Zebrafish embryos were injected with a PI3KC2α (PI3KC2α morphants) or a control morpholino (control morphants) at 1-cell stage. HEK293 with stable overexpression of GFP-tagged β2-AR were generated and transfected with either scramble or PI3KC2α siRNAs.

Results
Our findings indicate that PI3KC2α KO mice display a reduced cardiac contractility compared to wildtype animals. Similarly, PI3KC2α morphants zebrafish exhibit lower heart rate and fractional shortening compared to controls, both at baseline and after isoproterenol (ISO) stimulation. A similar unresponsiveness to ISO was found in vivo in PI3KC2α KO mice where chronic treatment with the β-adrenergic receptor (β-AR) agonist failed to induce the classical β-AR-mediated remodeling, characterized by an increase of the left ventricular mass and of cardiomyocyte area. These findings suggest that PI3KC2α plays a critical role in the regulation of β-AR signaling. In agreement, cAMP levels failed to increase in response to ISO treatment in PI3KC2α morphants. Furthermore, silencing of PI3KC2α in HEK293-GFP-β2-AR cells resulted in an increased GFP-β2-AR internalization compared to control cells, both at baseline and after ISO stimulation. Interestingly, preliminary findings indicate that overexpression of constitutively active Rab11Q70L induced a redistribution of GFP-β2-AR at the plasma membrane in PI3KC2α-silenced cells.

Conclusion
Overall, we identify a key role for PI3KC2α in the control of cardiac contractility through the regulation of β2-AR trafficking to the plasma membrane through a Rab11-dependent mechanism.
Mitochondrial players as novel therapeutic options in arrhythmogenic cardiomyopathy
Authors: Volani C , Medici A , Philippe R , Lavdas A , Della Corte I , Lang M , Frommelt L S , Blumer M , Sommariva E , Pompilio G , Troppmair J , Rossini A 
Published: May 2024

Abstract

Funding Acknowledgements
Type of funding sources: Public grant(s) – National budget only. Main funding source(s): Joint Project Südtirol- FWF (grant number 23623; Italy-Austria) and the Department of Innovation, Research and University of the Autonomous Province of Bolzano-South Tyrol (Italy)

Background
Mitochondria are dynamic organelles that sense and respond to environmental, nutritional, and pathological changes. These organelles can smartly adapt to different stressful conditions, by changing the way they network or the energy sources they use. Over the past years, numerous disorders have been associated with mitochondrial dysfunction, including cardiomyopathies. Arrhythmogenic Cardiomyopathy (ACM) is a cardiac disease characterized by the progressive replacement of the myocardium with fibro-fatty tissue. The current treatment primarily focuses on managing the symptoms rather than addressing the underlying issue of tissue substitution. Purpose: Therefore, in this study, we explored the mitochondrial and metabolic phenotype in ACM and tested the efficacy of a mitochondria-targeting library to rescue the fibro-fatty phenotype in ACM Methods: Human primary cardiac stromal cells (CStCs) were cultured in adipogenic medium (AM), a condition used to model the ACM fibro-fatty phenotype. Mitochondria-related analyses of ultrastructure, membrane potential, network connection, respiratory capacity, ROS production and redox system were performed at day (d) d0, d3, d7, d15 of AM exposure. A mitochondria-targeting library (MedChem Express) was tested on ACM-CStCs exposed to AM for 7 days. The extent of lipid accumulation was determined using the neutral lipid staining dye BODIPY 493/503, cell numbers were measured using Hoechst counterstaining, and images acquired using a high-content imaging system Results: At d0, the mitochondrial network was more fragmented in ACM compared to healthy CStCs (CTR). This result was in line with the observation of a decrease in mitochondrial respiratory capacity and an increase in ROS production. In addition, AM exposure led to faster and higher lipid accumulation in ACM CStCs, which was associated with higher mitochondrial oxygen consumption rates observed in ACM CStCs after 7 days of AM exposure. When ACM CStCs were simultaneously exposed to AM and 407 mitochondrial-directed compounds, about 25% of the compounds demonstrated the ability to reduce lipid accumulation Conclusion: ACM CStCs demonstrate an increased risk for mitochondrial network impairment. Moreover, prolonged exposure of ACM CStCs cells to AM negatively affects lipid deposition as well as mitochondrial and metabolic parameters. This highlights the potential for using specific mitochondria-targeting compounds to reduce lipid accumulation.
Caveolin-dependent endocytosis regulates arterial calcification
Authors: Gorgels A , Simon J , Hense N , Marx N , Goettsch C 
Published: May 2024

Abstract

Funding Acknowledgements
Type of funding sources: Public grant(s) – National budget only. Main funding source(s): SFB219

Introduction
Patients with chronic kidney disease (CKD) have a significantly elevated cardiovascular risk, which is primarily due to premature vascular aging with arterial calcification - a substantial public health burden. Calcifying extracellular vesicles (EVs) are required for the onset and progression of calcification. Endocytosis and exocytosis are important for EV genesis and cargo loading, thus controlling EV release and propensities.

Purpose
We aim to investigate endocytosis, endosomal ripening, and exocytosis as critical mechanisms of EV-dependent mineralization.

Methods and Results
First, we characterized EVs from calcifying vascular smooth muscle cells (SMCs) compared to control SMCs. Western blot analysis revealed an increase in tissue nonspecific alkaline phosphatase (TNAP) among calcifying EVs but not in flotillin abundance. When compared to control EVs, calcifying EVs showed higher TNAP activity. Flow cytometry and a turbidity assay were then used to evaluate EV mineral cargo and aggregation potential. Compared to the control, EVs produced from SMCs cultivated under calcifying conditions had 5-fold more mineral-positive EVs (p=0.004) and a 3-fold higher nucleation potential (p&lt;0.001). Next, endocytosis was blocked by targeting Dynamin-1 and –2, which inhibited caveolin- and clathrin-dependent endocytosis (Dynasore hydrate), or targeting clathrin-terminal domain, which inhibited solely clathrin-dependent endocytosis (Pitstop 2). Dynasore reduced matrix mineralization dose-dependently and lowered mineral-positive EVs by 18%, whereas Pitstop 2 had no effect, suggesting a role of caveolin-dependent endocytosis in EV-mediated calcification. Indeed, Cav-1 silencing inhibited matrix mineralization and Cav-1 protein was more abundant in calcifying EVs than in control EVs. Dynasore and Pitstop-2 did not affect the EV number measured by nanoparticle tracking analysis. Inhibition of endosomal ripening by Rab7 inhibition did not affect SMC calcification. Exocytosis was inhibited by Rab27a inhibition, which reduced mineral deposition (-32%, p=0.017) without affecting EV mineral or EV quantity.

Conclusion
EVs released from calcifying SMCs showed a higher abundance of mineral and TNAP proteins, suggesting a role in mineral transport and deposition. Our data suggest that caveolin-dependent endocytosis is essential in EV-mediated SMC calcification. Mineral cargo in EVs is independent of Rab7 and Rab27a pathways.
HiPSC-derived cardiomyocyte cultivation and measurement of electrophysiological properties on gelatine/glucose/polypyrrole scaffold
Authors: Vaiciuleviciute R , Alaburda A , Shelest A , Elkhoury K , Maziz A , Bergaud C , Schubert M , Guan K , Uzieliene I , Bernotiene E 
Published: May 2024

Abstract

Funding Acknowledgements
Type of funding sources: Public grant(s) – EU funding. Main funding source(s): EU Horizon 2020 Framework Programme (H2020) under Grant agreements No. 953138 (EMAPS-Cardio)

Purpose
Cardiotoxicity is one of the main side effects limiting development of new medicines and resulting in withdrawal of drugs from the market. There are different mechanisms of drug-induced cardiotoxicity, including arrhythmia, disruption of mitochondria function, apoptosis, altered growth factor signalling [1]. Heart on a chip model for drug testing seems attractive approach to evaluate the possible cardiotoxic effects on cell biological, but mostly electrophysiological properties. Our aim was to create a 3D system with a biocompatible scaffold, coated with a conductive layer, suitable for culturing and maturation of human induced pluripotent stem cell derived cardiomyocytes (hiPSC-CMs) and compatible with electrophysiology parameter measurement techniques.

Methods
Elastic scaffold was fabricated by electrospinning of gelatin-glucose microfibers followed by the vapor-phase and electrochemical deposition of conductive polymer polypyrrole (PPy) to create electroconductive layer. hiPSC-CMs at different maturation levels (at 6 vs 14 weeks) were used to test biocompatibility of the scaffold. To increase cell attachment, scaffold was treated with 0.1 M HCl and coated with Geltrex. Cells were stained with fluorescent dyes (Calcein AM, DAPI). Electrophysiological properties were analysed using intracellular calcium imaging (Cal520) and action potential recordings with sharp electrodes. HiPSC-CMs on coverslips were used as a 2D control.

Results
Calcein AM staining showed that high number of hiPSC-CMs attached to gelatine/glucose/PPy scaffold proving that the scaffold is biocompatible and cells remain viable even after long term incubation (&gt; 2 weeks). HiPSC-CMs at lower maturity (6 weeks) had better attachment properties to it, indicating that more mature cardiomyocytes (14 weeks) gradually lose their capacity to attach to PPy surface. Intracellular calcium imaging showed that both types of cells generated synchronous spontaneous calcium oscillations on gelatine/glucose/PPy scaffolds. Action potential recordings in 3D were similar to those in 2D, and confirmed differences in hiPSC-CMs maturation levels – at lower maturation level hiPSC-CMs had lower upstroke velocity as compared to more matured hiPSC-CMs.

Conclusions
Gelatine/glucose/PPy scaffold is biocompatible for hiPSC-CMs cultivation, and suitable for evaluation of cardiomyocyte electrophysiological properties. Such electromechanoactive scaffold can be further employed for development of heart-on-a-chip model.