bluuebunny/crossref_metadata_2025_split huggingface dataset:
PENERAPAN KONSEP BILANGAN UNTUK ANAK USIA DINI MELALUI KEGITAN MARKET DAY MENGGUNAKAN ‘BUY-PAY MONEY CARD’
Authors: Nuraeni Sinta , Muqodas Idat , Wulandari Hayani 
Published: June 2024

Banyaknya anak usia dini yang belum mengenal konsep bilangan seperti menghitung, membandingkan, mengurutkan dan mengenal lambang bilangan yang berdampak pada kecakapan hidup anak. Penelitian ini bertujuan untuk meningkatkan pemahaman konsep bilangan Anak Usia Dini melalui kegiatan Market Day menggunakan Buy-Pay Monay Card. Metode penelitian menggunakan Penelitian Tindakan Kelas (PTK) model Kemmis & Mc Taggart dengan subjek peneliti sejumlah 6 anak kelompok B usia 5-6 tahun. Data diperoleh melalui observasi (checklist) dari kemampuan konsep bilangan anak dan dokumentasi. Temuan penelitian menunjukkan bahwa kegiatan Market Day menggunakan Buy-Pay Money Card dilakukan dua siklus pada setiap siklus terdapat dua tindakan. Peningkatan dilihat pada hasil penelitian. Pada kondisi awal, kriteria Belum Berkembang (BB) sejumlah 5 anak (83,333%) . Mulai Berkembang (MB) sejumlah 1 anak (16,666%). Berkembangan sesuai harapan (BSH) 0 anak (0%). Berkembang Sangat Baik (BSB) 0 anak (0%), sedangakan pada kondisi hasil penelitian akhir kriteria Belum Berkembang (BB) 0 anak (0%). Mulai Berkembang (MB) sejumlah 0 anak (0%). Berkembangan sesuai harapan (BSH) sejumlah 1 anak (16,666%). Berkembang Sangat Baik (BSB) sejumlah 5 anak (83,333%). Dengan demikian, dapat disimpulkan bahwa kegiatan Market Day menggunakan Buy-Pay Monay Card dapat meningkatkan penguasaan konsep bilangan.
Rapid detection of Mycobacterium tuberculosis based on cyp141 via real-time fluorescence loop-mediated isothermal amplification (cyp141-RealAmp)
Authors: Zhu Yinyin , Feng Zi , Xu Yinfang , Luo Sha , Zhang Ruixian , Shi Xudong , Wu Xuping , Zhang Hongying 
Published: June 2024

BackgroundThe rapid detection of Mycobacterium tuberculosis (MTB) is essential for controlling tuberculosis.
Methods We designed a portable thermocycler-based real-time fluorescence loop-mediated isothermal amplification assay (cyp141-RealAmp) using six oligonucleotide primers derived from cyp141 to detect MTB. A combined number of 213 sputum samples (169 obtained from clinically diagnosed cases of pulmonary TB and 44 from a control group without tuberculosis) underwent Acid-fast bacillus (AFB) smear, culture, Xpert MTB/RIF assays, and cyp141-RealAmp assay.
ResultsBy targeting MTB cyp141, this technique could detect as low as 10 copies/reaction within 30 min, and it was successfully rejected by other mycobacteria and other bacterial species tested. Of the 169 patients, there was no statistical difference between the detection rate of cyp141-RealAmp (92.90%, 95% CI: 89.03–96.07) and that of Xpert MTB/RIF (94.67%, 95% CI: 91.28–98.06) (P &gt; 0.05), but both were statistically higher than that of culture (65.68%, 95% CI: 58.52–72.84) (P&lt; 0.05) and AFB (57.40%, 95% CI: 49.94–64.86) (P&lt; 0.05). Both cyp141-RealAmp and Xpert MTB/RIF had a specificity of 100%. Furthermore, a high concordance between cyp141-RealAmp and Xpert MTB/RIF was found (Kappa = 0.89).ConclusionThe cyp141-RealAmp assay was shown to be effective, responsive, and accurate in this study. This method offers a prospective strategy for the speedy and precise detection of MTB.
Analysis of gut microecological characteristics and differences between children with biliary atresia and non-biliary atresia in infantile cholestasis
Authors: Liu Yajun , Zhang Yuan , Guo Cheng , Li Muxia , Wang Ye , Zhang Lin 
Published: June 2024

IntroductionIn infants with cholestasis, variations in the enterohepatic circulation of bile acids and the gut microbiota (GM) characteristics differ between those with biliary atresia (BA) and non-BA, prompting a differential analysis of their respective GM profiles.MethodsUsing 16S rDNA gene sequencing to analyse the variance in GM composition among three groups: infants with BA (BA group, n=26), non-BA cholestasis (IC group, n=37), and healthy infants (control group, n=50). Additionally, correlation analysis was conducted between GM and liver function-related indicators.ResultsPrincipal component analysis using Bray–Curtis distance measurement revealed a significant distinction between microbial samples in the IC group compared to the two other groups. IC-accumulated co-abundance groups exhibited positive correlations with aspartate aminotransferase, alanine aminotransferase, total bilirubin, direct bilirubin, and total bile acid serum levels. These correlations were notably reinforced upon the exclusion of microbial samples from children with BA.ConclusionThe varying “enterohepatic circulation” status of bile acids in children with BA and non-BA cholestasis contributes to distinct GM structures and functions. This divergence underscores the potential for targeted GM interventions tailored to the specific aetiologies of cholestasis.
Anti-C1q antibodies: a biomarker for diagnosis and management of lupus nephritis. A narrative review
Authors: Calatroni Marta , Moroni Gabriella , Conte Emanuele , Stella Matteo , Reggiani Francesco , Ponticelli Claudio 
Published: June 2024

Nephritis is a frequent and severe complication of Systemic Lupus Erythematous (SLE). The clinical course of lupus nephritis (LN) is usually characterized by alternating phases of remission and exacerbation. Flares of LN can lead to deterioration of kidney function, necessitating timely diagnosis and therapy. The presence of autoantibodies against C1q (anti-C1qAb) in the sera of SLE patients has been reported in various studies. Some research suggests that the presence and changes in the titer of anti-C1qAb may be associated with the development of LN, as well as with LN activity and renal flares. However, the exact role of anti-C1qAb in LN remains a subject of debate. Despite variability in the results of published studies, anti-C1qAb hold promise as noninvasive markers for assessing LN activity in SLE patients. Measuring anti-C1qAb levels could aid in diagnosing and managing LN during periods of both inactive disease and renal flares. Nevertheless, larger controlled trials with standardized laboratory assays are necessary to further establish the utility of anti-C1qAb in predicting the reactivation and remission of LN and guiding treatment strategies.
Investigating the role of Caspase-1 in a mouse model of Juvenile X-linked Retinoschisis
Authors: Gehrke Ella J. , Pandey Araniko , Thompson Jacob , Bhattarai Sajag , Gurung Prajwal , Hsu Ying , Drack Arlene V. 
Published: June 2024

PurposePrevious studies have reported Caspase-1 (Casp1) is upregulated in mouse models of Juvenile X-linked Retinoschisis (XLRS), however no functional role for Casp1 in disease progression has been identified. We performed electroretinogram (ERG) and standardized optical coherence tomography (OCT) in mice deficient in the Retinoschisin-1 (Rs1) and Casp1 and Caspase-11 (Casp11) genes (Rs1-KO;Casp1/11−/−) to test the hypothesis that Casp1 may play a role in disease evolution and or severity of disease. Currently, no studies have ventured to investigate the longer-term effects of Casp1 on phenotypic severity and disease progression over time in XLRS, and specifically the effect on electroretinogram.MethodsRs1-KO;Casp1/11−/− mice were generated by breeding Rs1-KO mice with Casp1/11−/− mice. OCT imaging was analyzed at 2-, 4-, and 15–16 months of age. Outer nuclear layer (ONL) thickness and adapted standardized cyst severity score were measured and averaged from 4 locations 500 μm from the optic nerve. Adapted standardized cyst severity score was 1: absent cysts, 2: &lt;30 μm, 3: 30–49 μm, 4: 50–69 μm, 5: 70–99 μm, 6: &gt;99 μm. Electroretinograms (ERG) were recorded in dark-adapted and light-adapted conditions at 2 and 4 months. Results obtained from Rs1-KO and Rs1-KO;Casp1/11−/− eyes were compared with age matched WT control eyes at 2 months.ResultsIntraretinal schisis was not observed on OCT in WT eyes, while schisis was apparent in most Rs1-KO and Rs1-KO;Casp1/11−/− eyes at 2 and 4 months of age. There was no difference in the cyst severity score from 2 to 4 months of age, or ONL thickness from 2 to 16 months of age between Rs1-KO and Rs1-KO;Casp1/11−/− eyes. ERG amplitudes were similarly reduced in Rs1-KO and Rs1-KO;Casp1/11−/− compared to WT controls at 2 months of age, and there was no difference between Rs1-KO and Rs1-KO;Casp1/11−/− eyes at 2 or 4 months of age, suggesting no impact on the electrical function of photoreceptors over time in the absence of Casp1.ConclusionAlthough Casp1 has been reported to be significantly upregulated in Rs1-KO mice, our preliminary data suggest that removing Casp1/11 does not modulate photoreceptor electrical function or alter the trajectory of the retinal architecture over time.
Developing digital competencies of nursing professionals in continuing education and training – a scoping review
Authors: Tischendorf Tim , Hasseler Martina , Schaal Tom , Ruppert Sven-Nelson , Marchwacka Maria , Heitmann-Möller André , Schaffrin Sandra 
Published: June 2024

IntroductionThe German health and care system is transforming due to advancing digitalization. New technological applications in nursing, such as social and assistance robotics, artificial intelligence and legal framework conditions are increasingly focused in numerous research projects. However, the approaches to digitalization in nursing are very different. When integrating technologies such as robotics and artificial intelligence into nursing, it is particularly important to ensure that ethical and human aspects are taken into account. A structured classification of the development of digitalization in nursing care is currently hardly possible. In order to be able to adequately deal with this digital transformation, the acquisition of digital competences in nursing education programs is pivotal. These include the confident, critical and creative use of information and communication technologies in a private and professional context. This paper focuses on the question which specific training offers already exist at national and international level for nursing professions to acquire digital competences.MethodsA scoping review according to the PRISMA scheme was conducted in the PubMed and CINAHL databases. The search period for the scoping review extended from 2017 to 2024.ResultsThe selection of the studies took place by inclusion and exclusion criteria and the content-related orientation of the publications. After reviewing the titles and abstracts, eight studies were included. Of these, four were published in German-speaking countries and another four in international English-language journals.DiscussionThe topic of digitization of the nursing professions and the question of how nurses can acquire digital competences is gaining international attention. Nevertheless, the research on explicit continuing education programs for nursing professions is still undifferentiated. No specific continuing education offer for the development of digital competences of nursing professionals was identified. Many authors remained at the meta-level when developing methodological concepts for the acquisition of digital competences. The systematic integration of digitalization into higher education and continuing vocational training is mentioned in the publications. The development of theory- and research-based educational frameworks, which can be used as a basis for curricula in nursing studies and continuing education, is highly recommendable.
Profiles of apple allergen components and its diagnostic value in Northern China
Authors: Wang Xiaoyan , Chen Lijia , Lan Tianfei , Wang Hongtian , Wang Xueyan 
Published: June 2024

BackgroundLimited is known on the profiles of apple allergy in China.ObjectiveTo explore the clinical significance of apple allergen components in northern China.MethodsThis study recruited 40 participants and categorized into apple tolerance (n = 19) and allergy (n = 21) group. The latter was categorized into oral allergy symptoms (OAS, n = 14) and generalized symptoms (GS, n = 7). All participants underwent ImmunoCAP screening to assess sIgE levels of birch, apple, and their components.ResultsThe sensitization rates were 90% for Bet v 1, 85% for Mal d 1, 35% for Bet v 2, and 20% for Mal d 3. The overall positive rate for apple allergens was 97.5%, with half demonstrating mono-sensitization to Mal d 1. Birch, Bet v 1 and Mal d 1 sIgE levels had consistent areas under the curve (AUC 0.747, p = 0.037; AUC 0.799, p = 0.012; AUC 0.902, p &lt; 0.001 respectively) in diagnosing apple allergy. The optimal cut-off values were determined to be 22.85 kUA/L (63.6% sensitivity, 85.7% specificity), 6.84 kUA/L (81.8% sensitivity, 71.4% specificity) and 1.61 kUA/L (93.8% sensitivity, 75.0% specificity), respectively. No allergens or components demonstrated diagnostic value in distinguishing between OAS and GS. Mal d 3 sensitization was correlated with mugwort allergy and higher risk of peach, nuts or legumes generalized allergy.ConclusionMal d 1 was major allergen and the best for diagnosing apple allergy. Mal d 3 does not necessarily indicate severe allergic reaction to apples in northern China but may indicate mugwort sensitization and an increased risk of peach, nuts or legumes allergy.
Case report: Clinical features of pediatric acute myeloid leukemia presenting with cardiac tamponade: a case series study and literature review
Authors: Li Tonghui , Tang Xue , He Xuezhi , Zhang Lei , Zhang Ya , Wang Lulu , Liu Shilin , Zhou Guichi , Wen Feiqiu , Liu Sixi , Mai Huirong , Wang Ying 
Published: June 2024

ObjectiveThis study aims to elucidate the clinical features observed in cases of pediatric acute myeloid leukemia (AML) initially presenting with cardiac tamponade and to share treatment experiences.Materials and methodsFive pediatric patients were initially diagnosed with AML accompanied by cardiac myeloid sarcoma (MS). The diagnosis was established by examining our hospital records and reviewing pertinent literature from 1990 to July 2023, accessible through MEDLINE/PubMed. We comprehensively assessed the clinical characteristics and treatment modalities employed for these patients.ResultFive pediatric patients presented with acute symptoms, including shortness of breath, malaise, cough, and fever, leading to their hospitalization. Physical examination revealed irritability, hypoxia, tachypnea, tachycardia, and hypotension. Initial detection utilized chest X-ray or echocardiogram, leading to subsequent diagnoses based on pericardial effusion and/or bone marrow examination. Two patients received chemotherapy at the time of initial diagnosis, one with cytarabine and etoposide, and the other with cytarabine and cladribine. Post-treatment, their bone marrow achieved remission, and over a 2.5-year follow-up, their cardiac function remained favorable. Unfortunately, the remaining three patients succumbed within two weeks after diagnosis, either due to receiving alternative drugs or without undergoing chemotherapy.ConclusionThis is the first and largest case series of pediatric AML patients with cardiac MS, manifesting initially with cardiac tamponade. It highlights the rarity and high mortality associated with this condition. The critical factors for reducing mortality include identifying clinical manifestations, conducting thorough physical examinations, performing echocardiography promptly, initiating early and timely pericardial drainage, and avoiding cardiotoxic chemotherapy medications.
Orphan drug policy analysis in China
Authors: Liu Meilin , Lu Yanqin , Li Junfeng , Zhang Yongtao , Zhang Shanshan , Liu Yisheng 
Published: June 2024

Rare diseases have various types, low incidence rates, complex conditions, and are often difficult to diagnose. Due to China’s large population, there is a significant number of rare disease patients, but there is a shortage of orphan drugs. Consequently, these patients often find themselves in a situation where necessary medications are either unavailable or unaffordable. To address this urgent clinical need, China has implemented a series of orphan drug policies aimed at improving drug accessibility and affordability. In terms of drug accessibility, companies are encouraged to expedite drug development through the implementation of tax incentives, guidance for clinical research on rare diseases, and the provision of data protection periods of 6 years, along with market exclusivity periods limited to a maximum of 7 years. Moreover, exemptions for clinical trials, acceptance of overseas clinical trial data, and the creation of a list prioritizing clinically urgent new drugs from overseas have been introduced to expedite the drug registration application, review, inspection, and approval processes. In terms of drug affordability, the import value-added tax on rare disease drugs has been reduced by 3%, and various provinces and cities have established a representative rare disease protection model, which includes special funds, medical assistance programs, and serious disease insurance. The national medical insurance catalog has been adjusted to reduce the financial burden on rare disease patients, resulting in an increase in the number of orphan drugs covered by the catalog to 95 as of March 2024. By comparing orphan drug policies in the United States, the European Union, Japan, Australia, and other countries (or regions), we will provide relevant suggestions to further improve orphan drug policies in China, thus bringing more treatment options and hope to patients with rare diseases.
A real-world disproportionality analysis of Tivozanib data mining of the public version of FDA adverse event reporting system
Authors: Wang Kaixuan , Wang Mengmeng , Li Wensheng , Wang Xiaohui 
Published: June 2024

BackgroundTivozanib, a vascular endothelial growth factor tyrosine kinase inhibitor, has demonstrated efficacy in a phase III clinical trials for the treatment of renal cell carcinoma. However, comprehensive evaluation of its long-term safety profile in a large sample population remains elusive. The current study assessed Tivozanib-related adverse events of real-world through data mining of the US Food and Drug Administration Adverse Event Reporting System FDA Adverse Event Reporting System.MethodsDisproportionality analyses, utilizing reporting odds ratio proportional reporting ratio Bayesian confidence propagation neural network and multi-item gamma Poisson shrinker (MGPS) algorithms, were conducted to quantify signals of Tivozanib-related AEs. Weibull distribution was used to predict the varying risk incidence of AEs over time.ResultsOut of 5,361,420 reports collected from the FAERS database, 1,366 reports of Tivozanib-associated AEs were identified. A total of 94 significant disproportionality preferred terms (PTs) conforming to the four algorithms simultaneously were retained. The most common AEs included fatigue, diarrhea, nausea, blood pressure increased, decreased appetite, and dysphonia, consistent with prior specifications and clinical trials. Unexpected significant AEs such as dyspnea, constipation, pain in extremity, stomatitis, and palmar-plantar erythrodysaesthesia syndrome was observed. The median onset time of Tivozanib-related AEs was 37 days (interquartile range [IQR] 11.75–91 days), with a majority (n = 127, 46.35%) occurring within the initial month following Tivozanib initiation.ConclusionOur observations align with clinical assertions regarding Tivozanib’s safety profile. Additionally, we unveil potential novel and unexpected AE signatures associated with Tivozanib administration, highlighting the imperative for prospective clinical studies to validate these findings and elucidate their causal relationships. These results furnish valuable evidence to steer future clinical inquiries aimed at elucidating the safety profile of Tivozanib.
Diversity of Colletotrichum species associated with anthracnose on Euonymus japonicus and their sensitivity to fungicides
Authors: Liu Yayong , Tan Xiaoqian , Zhao Juan , Niu Yajie , Hsiang Tom , Yu Zhihe , Qin Wentao 
Published: June 2024

As an evergreen shrub, Euonymus japonicus plays a crucial role in urban landscape construction, and its growth is affected by severe foliar anthracnose caused by Colletotrichum spp. However, the biodiversity of Colletotrichum species associated with anthracnose on E. japonicus remains undetermined. This study involved a two-year collection of E. japonicus leaf samples with typical anthracnose symptoms from 9 districts in Beijing, China. A total of 194 Colletotrichum isolates were obtained, and eight Colletotrichum species were subsequently identified using morphological characteristics and molecular identification with the ACT, GADPH, CHS, TUB2, and CAL genes, as well as the rDNA-ITS region. These species included Colletotrichum aenigma, C. fructicola, C. gloeosporioides, C. grossum, C. hebeiense, C. karstii, C. siamense, and C. theobromicola with C. siamense being the most prevalent (57%), followed by C. aenigma and C. theobromicola. Furthermore, C. fructicola, C. grossum and C. hebeiense are reported for the first time as causal agents of anthracnose on E. japonicus worldwide, and C. karstii is newly reported to be associated with E. japonicus anthracnose in China. Pathogenicity tests revealed that all tested isolates exhibited pathogenicity in the presence of wounds, emphasizing the need to avoid artificial or mechanical wounds to prevent infection in E. japonicus management. The EC50 values of five fungicides, namely difenoconazole, flusilazole, tebuconazole, hexaconazole, and prochloraz, were found to be less than 10 mg/L, indicating their strong potential for application. Notably, the EC50 of prochloraz was less than 0.05 mg/L for C. theobromicola. These findings offer valuable insights for the management of anthracnose on E. japonicus.
Catalysts of violence against women students: the role of the university, aggressors, and victims
Authors: Barbosa Aline dos Santos , Romani-Dias Marcello , Veludo-de-Oliveira Tania 
Published: June 2024

PurposeThe purpose of this study is to bring a multilevel perspective to the discussion of the antecedents of violence against women in higher education settings.Originality/valueThis paper was guided by the need indicated in the literature for research on the multiple levels that constitute the context of violence against women, as this is a public health problem, a designation that indicates the urgency with which this pervasive phenomenon should be addressed. The university context is conducive to this type of research, as it includes situations that favor instances of violence. Additionally, it aligns with the United Nations Sustainable Development Goals (SDGs) of Gender Equality and Quality Educations.Design/methodology/approachThis paper follows a qualitative and interpretative approach. This choice was due to the need to know the “how” and “why” elements that are part of violence against women in the university context. As the main source of evidence for the study, we conducted 20 in-depth interviews with women (victims) and men (aggressors), all university students involved in situations of violence. The transcription of the interviews generated 346 quotations, including 41 analysis codes.FindingsAfter conducting the data coding, we identified that (i) the actions and omissions of the educational institution, (ii) the taste for violence, the perception of self-efficacy and the influence of the aggressors’ group of friends, and (iii) the apparent dichotomy between women’s vulnerability and women’s strength are among the main antecedents of violence against women. The article concludes with possible research questions to combat violence. Among the contribution of the discussions presented in our article, we highlight the importance of adopting a multilevel view so that we can better understand and fight against this violence, the existence of which is not restricted to the university context.