bluuebunny/crossref_metadata_2025_split huggingface dataset:
Material extrusion additive manufacturing of zirconia: from filament characterisation to Weibull statistics
Authors: Morfini Luigi , Meng Fankai , Beretta Margherita , Vleugels Jozef , Spina Roberto , Ferraris Eleonora 
Published: April 2024

Purpose
This study aims to investigate the performance of filament-based material extrusion additive manufacturing (MEX), combined with debinding and sintering, as a novel approach to manufacturing ceramic components.

Design/methodology/approach
A commercial ZrO2 filament was selected and analysed by infra-red (IR) spectroscopy, rheology and thermo-gravimetry. The influence of the print parameters (layer thickness, flow rate multiplier, printing speed) and sintering cycle were investigated to define a suitable printing and sintering strategy. Biaxial flexure tests were applied on sintered discs realised with optimised printing strategies, and the results were analysed via Weibull statistics to evaluate the mechanical properties of printed components. The hardness and thermal conductivity of sintered components were also tested.

Findings
Layer thickness and flow rate multiplier of the printing process were proved to have significant effect on the density of as-printed parts. Optimised samples display a sintered density >99% of the theoretical density, 20% linear sintering shrinkage, a characteristic flexural strength of 871 MPa with a Weibull modulus of 4.9, a Vickers hardness of 12.90 ± 0.3 GPa and a thermal conductivity of 3.62 W/mK. Gyroids were printed for demonstration purposes.

Originality/value
To the best of the authors’ knowledge, this work is the first to apply biaxial flexure tests and Weibull statistics to additively manufactured MEX zirconia components, hence providing comparable results to other additive technologies. Moreover, fractography analysis builds the connection between printing defects and the fracture mechanism of bending. This study also provides guidelines for fabricating high-density zirconia components with MEX.
A new mechanism for crisis management success
Authors: Hosen Mosharrof , Liew Chee Yoong , Lim Ai‐Fen , Hameed Irfan , Rehman Hafiz Mudassir 
Published: April 2024

AbstractThe goal of this study is to present a thorough analysis of the ways that organisational culture (OC) and risk assessment competencies of leaders affect the creation of strategic frameworks, especially about high‐quality crisis decision‐making. A self‐administered questionnaire was distributed to 436 organisational personnel; 304 valid responses were received, and these were analysed using variance‐based structural equation modelling. The findings show that OC, leadership risk assessment and a strategic management plan (SMP) are favourable predictors of high‐quality crisis decision‐making. According to this research, every variable strengthens the link between SMP quality and crisis decisions. The study is supported by an integrated method for understanding complex organisational facts. Practical ramifications for leadership development include strengthening leaders' capacity to appraise risk, considering OC and employing an SMP to guarantee that, when making sound crisis judgements, they are both innovative and compliant with organisational ethics.
Enhancement of acyl‐CoA precursor supply for increased avermectin B1a production by engineering meilingmycin polyketide synthase and key primary metabolic pathway genes
Authors: Yang Mengyao , Hao Yi , Liu Gang , Wen Ying 
Published: April 2024

AbstractAvermectins (AVEs), a family of macrocyclic polyketides produced by Streptomyces avermitilis, have eight components, among which B1a is noted for its strong insecticidal activity. Biosynthesis of AVE “a” components requires 2‐methylbutyryl‐CoA (MBCoA) as starter unit, and malonyl‐CoA (MalCoA) and methylmalonyl‐CoA (MMCoA) as extender units. We describe here a novel strategy for increasing B1a production by enhancing acyl‐CoA precursor supply. First, we engineered meilingmycin (MEI) polyketide synthase (PKS) for increasing MBCoA precursor supply. The loading module (using acetyl‐CoA as substrate), extension module 7 (using MMCoA as substrate) and TE domain of MEI PKS were assembled to produce 2‐methylbutyrate, providing the starter unit for B1a production. Heterologous expression of the newly designed PKS (termed Mei‐PKS) in S. avermitilis wild‐type (WT) strain increased MBCoA level, leading to B1a titer 262.2 μg/mL – 4.36‐fold higher than WT value (48.9 μg/mL). Next, we separately inhibited three key nodes in essential pathways using CRISPRi to increase MalCoA and MMCoA levels in WT. The resulting strains all showed increased B1a titer. Combined inhibition of these key nodes in Mei‐PKS expression strain increased B1a titer to 341.9 μg/mL. Overexpression of fatty acid β‐oxidation pathway genes in the strain further increased B1a titer to 452.8 μg/mL – 8.25‐fold higher than WT value. Finally, we applied our precursor supply strategies to high‐yield industrial strain A229. The strategies, in combination, led to B1a titer 8836.4 μg/mL – 37.8% higher than parental A229 value. These findings provide an effective combination strategy for increasing AVE B1a production in WT and industrial S. avermitilis strains, and our precursor supply strategies can be readily adapted for overproduction of other polyketides.
Alcohol consumption and accelerated biological ageing in middle‐aged and older people: A longitudinal study from two cohorts
Authors: Chen Hongxiang , Yin Jianzhong , Xiang Yi , Zhang Ning , Huang Zitong , Zhang Yuan , Tang Dan , Wang Ziyun , Baimayangji  , Chen Liling , Jiang Xiaoman , Xiao Xiong , Zhao Xing 
Published: April 2024

AbstractBackground and aimsThe relationship between alcohol consumption and age‐related diseases is inconsistent. Biological age (BA) serves as both a precursor and a predictor of age‐related diseases; however, longitudinal associations between alcohol consumption and BA in middle‐aged and older people remain unclear. We measured whether there was a longitudinal association between drinking frequency and pure alcohol intake with BA among middle‐aged and older people.Design and setting and participantsThis study involved two prospective cohort studies, set in Southwestern China and the United Kingdom. A total of 8046 participants from the China Multi‐Ethnic Cohort study (CMEC) and 5412 participants from the UK Biobank (UKB), aged 30–79 years, took part, with complete data from two waves of clinical biomarkers.MeasurementsBA was calculated by the Klemera Doubal's method. Accelerated BA equalled BA minus chronological age. Drinking frequency and pure alcohol intake were obtained through self‐reported questionnaires. Drinking frequency in the past year was classified as current non‐drinking, occasional (monthly drinking) and regular (weekly drinking).FindingsCompared with consistent current non‐drinkers, more frequent drinkers [CMEC: β = 0.46, 95% confidence interval (CI) = 0.13–0.80; UKB: β = 0.65, 95% CI = 0.01–1.29)], less frequent drinkers (CMEC: β = 0.62, 95% CI = 0.37–0.87; UKB: β = 0.54, 95% CI = −0.01–1.09), consistent occasional drinkers (CMEC: β = 0.51, 95% CI = 0.23–0.79; UKB: β = 0.63, 95% CI = 0.13–1.13) and consistent regular drinkers (CMEC: β = 0.56, 95% CI = 0.17–0.95; UKB: β = 0.46, 95% CI = 0.00–0.91) exhibited increased accelerated BA. A non‐linear relationship between pure alcohol intake and accelerated BA was observed among consistent regular drinkers.ConclusionsIn middle‐aged and older people, any change in drinking frequency and any amount of pure alcohol intake seem to be positively associated with acceleration of biological ageing, compared with maintaining abstinence.
The social life of illegality: Suspicion and surveillance against African migrants in urban India
Authors: Gill Bani 
Published: April 2024

AbstractThe turn of the 21st century has witnessed a rising trend of migration from the African continent to cities across India. Accompanying such flows have been racial tensions and policing spectacles, including incidents of violence, vandalism, and evictions against African migrants and their pathologization as “illegal.” These subtle yet pervasive forms of migrant policing by state and citizen actors constitute what I call the social life of “illegality” that is characterized by distinctive modes of suspicion and surveillance. Based upon ethnography conducted in an “unplanned” settlement of Delhi cohabitated by both African and Indian residents, I illuminate how caste‐race‐religion informed indexes of difference contribute to the multi‐sensorial racialization of African migrants as suspicious. In emplacing such dynamics within changing spatial economies and the moral anxieties accompanying such transitions, I further demonstrate quotidian practice of microsurveillance against African migrants as sustaining their position as rent‐paying clients who are nonetheless maintained in their racial alterity. The social life of “illegality” thus refocuses attention on the sensorial and emplaced registers that illegalize migrants, above and beyond documentation, thereby furthering a discussion on migrant “illegality” as enmeshed within racialized imaginaries, urban transformations, and alternate modes of governmentality.
Cholecystectomy in the red centre: a review of the surgical outcomes in Central Australia in a five‐year period
Authors: Barbaro Antonio , Paredes Steven Ronald , Tran Steven , Kaur Harleen , Arayne Aisha Abdulmoeed , Senaratne Jayantha 
Published: April 2024

AbstractBackgroundDespite the high rates of cholecystectomy in Australia, there is minimal literature regarding the outcomes of cholecystectomy in rural Central Australia within the Northern Territory. This study aims to better characterize the outcomes for patients undergoing cholecystectomy in Central Australia and review clinical and patient characteristics, which may affect outcomes.MethodA retrospective case–control study was performed using data obtained from medical records for all patients undergoing cholecystectomy at Alice Springs Hospital in the Northern Territory from January 2018 until December 2022. Patient characteristics were gathered, and key outcomes examined included: inpatient mortality and 30‐day mortality, bile duct injury, bile leak, return to theatre, conversion to open, duration of procedure, length of stay, and up‐transfer to a tertiary referral centre.ResultsA total of 466 patients were included in this study. Majority of the patients were female and there was a large portion of Indigenous Australians (56%). There were no inpatient mortalities, or 30‐day mortalities recorded. There were two bile leaks and/or bile duct injuries (0.4%) and two unplanned returned to theatres (0.4%). Indigenous Australians were more likely to require an emergency operation and had a longer median length of stay (P < 0.001).ConclusionCholecystectomy can be performed safely and to a high standard in Central Australia. Surgeons in Central Australia must appreciate the nuances in the management of patients who come from a significantly different socioeconomic background, with complex medical conditions when compared to metropolitan centres.
Autocrine activation of P2X7 receptors mediates catecholamine secretion in chromaffin cells
Authors: Maldifassi María Constanza , Guerra‐Fernández María José , Ponce Daniela , Alfonso‐Bueno Samuel , Maripillán Jaime , Vielma Alex H. , Báez‐Matus Ximena , Marengo Fernando D. , Acuña‐Castillo Claudio , Sáez Juan C. , Martínez Agustín D. , Cárdenas Ana M. 
Published: April 2024

AbstractBackground and PurposeATP is highly accumulated in secretory vesicles and secreted upon exocytosis from neurons and endocrine cells. In adrenal chromaffin granules, intraluminal ATP reaches concentrations over 100 mM. However, how these large amounts of ATP contribute to exocytosis has not been investigated.Experimental ApproachExocytotic events in bovine and mouse adrenal chromaffin cells were measured with single cell amperometry. Cytosolic Ca2+ measurements were carried out in Fluo‐4 loaded cells. Submembrane Ca2+ was examined in PC12 cells transfected with a membrane‐tethered Ca2+ indicator Lck‐GCaMP3. ATP release was measured using the luciferin/luciferase assay. Knockdown of P2X7 receptors was induced with short interfering RNA (siRNA). Direct Ca2+ influx through this receptor was measured using a P2X7 receptor‐GCamp6 construct.Key ResultsATP induced exocytosis in chromaffin cells, whereas the ectonucleotidase apyrase reduced the release events induced by the nicotinic agonist dimethylphenylpiperazinium (DMPP), high KCl, or ionomycin. The purinergic agonist BzATP also promoted a secretory response that was dependent on extracellular Ca2+. A740003, a P2X7 receptor antagonist, abolished secretory responses of these secretagogues. Exocytosis was also diminished in chromaffin cells when P2X7 receptors were silenced using siRNAs and in cells of P2X7 receptor knockout mice. In PC12 cells, DMPP induced ATP release, triggering Ca2+ influx through P2X7 receptors. Furthermore, BzATP, DMPP, and KCl allowed the formation of submembrane Ca2+ microdomains inhibited by A740003.Conclusion and ImplicationsAutocrine activation of P2X7 receptors constitutes a crucial feedback system that amplifies the secretion of catecholamines in chromaffin cells by favouring submembrane Ca2+ microdomains.
Long noncoding RNA SNHG3 interacts with microRNA‐502‐3p to mediate ITGA6 expression in liver hepatocellular carcinoma
Authors: Guo Juncheng , Zhang Jianquan , Xiang Yang , Zhou Shuai , Yang Yijun , Zheng Jinfang 
Published: April 2024

AbstractSNHG3, a long noncoding RNA (lncRNA), has been linked to poor outcomes in patients with liver hepatocellular carcinoma (LIHC). In this study, we found that SNHG3 was overexpressed in LIHC and associated with poor outcomes in patients with LIHC. Functional assays, including colony formation, spheroid formation, and in vivo assays showed that SNHG3 promoted stemness of cancer stem cells (CSC) and tumor growth in vivo by interacting with microRNA‐502‐3p (miR‐502‐3p). miR‐502‐3p inhibitor repressed the tumor‐suppressing effects of SNHG3 depletion. Finally, by RNA pull‐down, dual‐luciferase reporter assay, m6A methylation level detection, and m6A‐IP‐qPCR assays, we found that miR‐502‐3p targeted YTHDF3 to regulate the translation of integrin alpha‐6 (ITGA6) and targeted HBXIP to inhibit the m6A modification of ITGA6 through methyltransferase‐like 3 (METTL3). Our study revealed that SNHG3 controls the YTHDF3/ITGA6 and HBXIP/METTL3/ITGA6 pathways by repressing miR‐502‐3p expression to sustain the self‐renewal properties of CSC in LIHC.
Cutibacterium acnes‐derived extracellular vesicles promote tumor growth in renal cell carcinoma
Authors: Jingushi Kentaro , Kawashima Atsunari , Tanikawa Sayaka , Saito Takuro , Yamamoto Akinaru , Uemura Toshihiro , Sassi Nesrine , Ishizuya Yu , Yamamoto Yoshiyuki , Kato Taigo , Hatano Koji , Hase Hiroaki , Nonomura Norio , Tsujikawa Kazutake 
Published: April 2024

AbstractBacterial flora are present in various parts of the human body, including the intestine, and are thought to be involved in the etiology of various diseases such as multiple sclerosis, intestinal diseases, cancer, and uterine diseases. In recent years, the presence of bacterial 16S rRNA genes has been revealed in blood, which was previously thought to be a sterile environment, and characteristic blood microbiomes have been detected in various diseases. However, the mechanism and the origin of the bacterial information are unknown. In this study, we performed 16S rRNA metagenomic analysis of bacterial DNA in serum extracellular vesicles from five healthy donors and seven patients with renal cell carcinoma and detected Cutibacterium acnes DNA as a characteristic bacterial DNA in the serum extracellular vesicles of patients with renal cell carcinoma. In addition, C. acnes DNA was significantly reduced in postoperative serum extracellular vesicles from patients with renal cell carcinoma compared with that in preoperative serum extracellular vesicles from these patients and was also detected in tumor tissue and extracellular vesicles from tumor tissue‐associated microbiota, suggesting an association between C. acnes extracellular vesicles and renal cell carcinoma. C. acnes extracellular vesicles were taken up by renal carcinoma cells to enhance their proliferative potential. C. acnes extracellular vesicles also exhibited tumor‐promoting activity in a mouse model of renal cancer allografts with enhanced angiogenesis. These results suggest that extracellular vesicles released by C. acnes localized in renal cell carcinoma tissues act in a tumor‐promoting manner.
Syndromic craniosynostosis caused by a novel missense variant in MAP4K4: Expanding the genotype–phenotype relationship in RASopathies
Authors: Yoon Jihoon G. , Yu Jung Woo , Shim Kyu Won , Kim Yong Oock , Lee Min Goo 
Published: April 2024

AbstractRASopathies represent a distinct class of neurodevelopmental syndromes caused by germline variants in the Ras/MAPK pathways. Recently, a novel disease‐gene association was implicated in MAPK kinase kinase kinase 4 (MAP4K4), which regulates the upstream signals of the MAPK pathways. However, to our knowledge, only two studies have reported the genotype–phenotype relationships in the MAP4K4‐related disorder. This study reports on a Korean boy harboring a novel de novo missense variant in MAP4K4 (NM_001242559:c.569G>T, p.Gly190Val), revealed by trio exome sequencing, and located in the hotspot of the protein kinase domain. The patient exhibited various clinical features, including craniofacial dysmorphism, language delay, congenital heart defects, genitourinary anomalies, and sagittal craniosynostosis. Our study expands the phenotypic association of the MAP4K4‐related disorder to include syndromic craniosynostosis, thereby providing further insights into the role of the RAS/MAPK pathways in the development of premature fusion of calvarial sutures.
The use and abuse of moral theories in conservation debate about killing animals
Authors: Coghlan Simon , Cardilini Adam 
Published: April 2024

AbstractRecent ethical debate about compassionate conservation has invoked moral theories to oppose or support traditional practices of killing animals to protect biodiversity and ecosystems. The debate has featured the mainstream moral theories of consequentialism and utilitarianism, deontology, and virtue ethics. We identify problematic applications and critique of these moral theories in conservation discussions. Problems include a lack of clarity when invoking moral theories, misunderstanding and mischaracterizing theories, and overlooking features and circumstances affecting a theory's application. A key omission in the debate is a detailed discussion of the moral significance of animals and nature. We then examine the role of moral theory as such in ethical discussion, contrasting moral theory with ethical outlooks that center, for example, forms of love and care. Our aim is to advance the ethical debate about harming animals in conservation.
“They don't trust me”: Service providers reflections of father‐centric treatment and engagement
Authors: Garcia Antonio , Pérez‐Figueroa Rafael , Cozart David , Cook Victoria , Damron Jeff , Clark Shelby , Eubank Kendra 
Published: April 2024

AbstractObjectiveThis study sought to illuminate the barriers service providers experience in delivering healing services to enhance father and child well‐being.BackgroundManifestations of structural barriers, such as racism, mother‐centric practices, and disproportionate incarceration among Black and Brown fathers prevail. Little is known, however, about how providers describe barriers they encounter toward engaging fathers in services to equip them with the capacity to coparent effectively.MethodA convenience sample of providers (n = 24) representing a diverse array of child and family service systems responded to questions from a semistructured interview guide. Thematic analyses were conducted to describe how providers engage with fathers and their perceptions of barriers related to their ability to engage them in services.ResultsFindings revealed three types of barriers, each representing a separate theme: micro (biases, colorblindness), societal (devaluation of fathers' role), and systemic (racism, mother‐centrism). Strategies to address barriers included implementing strengths‐based practices, creating spaces to engage in reflexivity, and investing in father‐centric programming. Most strategies involved modifying the micro context, conveying that future efforts should focus on developing procedures and policies that will enhance micro practices.ConclusionThis study captured the experiences of change agents and healers working with fathers and families. Their experiences illuminate obstacles they must contend with at the micro, societal, and systemic levels of practice.ImplicationsProviders indicated that fathers will not benefit from services unless organizations embrace father‐centric, strength‐based healing practices and allocate space to engage in reflexivity about biases and the impact of systemic racism.
14‐day tailored PCR‐guided triple therapy versus 14‐day non‐Bismuth concomitant quadruple therapy for Helicobacter pylori eradication: A multicenter, open‐label randomized noninferiority controlled trial
Authors: Amiot Aurelien , Hacoon Jérémy , Heluwaert Frederic , Mion François , Lamarque Dominique , Moussata Driffa , Mimouni Maroua , Delchier Jean‐Charles , Durand‐Zaleski Isabelle , Audureau Etienne , Bastuji‐Garin Sylvie ,   for the HEPYSE Study Group
Published: April 2024

AbstractBackgroundThe systematic use of susceptibility testing and tailored first‐line treatment for Helicobacter pylori eradication has yet to be established.AimTo compare 14‐day tailored PCR‐guided triple therapy to 14‐day non‐Bismuth concomitant quadruple therapy for first‐line Helicobacter pylori eradication.Patients and MethodsWe performed a multicenter, parallel‐group, randomized noninferiority controlled trial. Naive adult patients with Helicobacter pylori infection were treated with 14‐day tailored PCR‐guided triple therapy (esomeprazole 40 mg and amoxicillin 1000 mg b.d. plus clarithromycin 500 mg or levofloxacin 500 mg b.d. according to clarithromycin susceptibility) or 14‐day non‐Bismuth concomitant quadruple therapy (esomeprazole 40 mg, amoxicillin 1000 mg, clarithromycin 500 mg, and metronidazole 500 mg b.d.). The primary endpoint was H. pylori eradication.ResultsWe screened 991 patients for eligibility and randomized 241 patients. The first‐line eradication rate was 99.2% in the tailored PCR‐guided group and 95.9% in the control group (ITT population; absolute difference of +3.30%, with a lower bound of CI at −0.68%). Both first‐line therapies were well tolerated, with a formally significant difference in favor of the tailored PCR‐guided group (61.4% vs. 41.2%, p = 0.003). Economic analyses revealed a lower cost of the tailored PCR‐guided arm, with a 92% chance of being jointly more effective and less expensive than the control arm in the ITT population.ConclusionIn a country with a high level of clarithromycin resistance, the results of our study demonstrated the noninferiority of 14‐day tailored PCR‐guided triple therapy as a first‐line H. pylori eradication therapy compared to 14‐day non‐Bismuth quadruple therapy (ClinicalTrials.gov NCT02576236).