bluuebunny/crossref_metadata_2025_split huggingface dataset:
Novel insights into the mechanism of decreased expression of type X collagen in human mesenchymal stem cells from patients with osteoarthritis cultured on nitrogen‐rich plasma polymers: Implication of cyclooxygenase‐1
Authors: Petit Alain , Wang Hong Tian , Girard‐Lauriault Pierre‐Luc , Wertheimer Michael R. , Antoniou John , Mwale Fackson 
Published: March 2010

AbstractRecent evidence indicates that a major drawback of current cartilage‐ and intervertebral disc (IVD) tissue engineering is that human mesenchymal stem cells (MSCs) from patients with osteoarthritis rapidly express type X collagen (COL10A1), a marker of late stage chondrocyte hypertrophy associated with endochondral ossification. We recently demonstrated that COL10A1 expression was inhibited in MSCs from patients with osteoarthritis cultured on nitrogen‐rich plasma polymerized (PPE:N) coatings. Here, we sought to understand the mechanisms of action of this effect by culturing MSCs on PPE:N surfaces in the presence of different inhibitors of kinases and cyclooxygenases. The effect of PPE:N surfaces on COL10A1 expression was found to be mimicked by the cyclooxygenase inhibitor NPPB, but not by daphnetin (an inhibitor of protein kinases) nor by genistein (an inhibitor of tyrosine kinases). COL10A1 expression was also suppressed by the specific cyclooxygenase‐1 (COX‐1: SC‐560) and 5‐lipoxygenase (5‐LOX: MK‐866) inhibitors, but not by COX‐2 (COX‐2 inhibitor 2) and 12‐LOX (baicalein) inhibitors. Finally, the incubation of MSCs on PPE:N surfaces inhibited the expression of COX‐1 while 5‐LOX was not expressed in these cells. Taken together, these results indicate that PPE:N surfaces inhibit COL10A1 expression via the suppression of COX‐1. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2010
Effect of two restorative materials on root dentine erosion
Authors: Domiciano Silvia Jorge , Colucci Vivian , Campos Serra Mônica 
Published: March 2010

AbstractThis study sought to evaluate the microhardness of root dentine adjacent to glass‐ionomer and composite resin restorations after erosive challenge. A crossover study was performed in two phases of 4 consecutive days each. One hundred twelve bovine root dentine slabs were obtained, and standardized box‐shaped cavities were prepared at center of each specimen. The prepared cavities were randomly restored with glass‐ionomer cement or composite resin. The slabs were randomly assigned among 14 volunteers, which wore intraoral palatal device containing four restored root dentin slabs. Starting on the second day, half of the palatal acrylic devices were immersed extraorally in a lemonade‐like carbonated soft drink for 90 s, four times daily for 3 days. After 3‐day wash‐out, dentine slabs restored with the alternative material were placed into palatal appliance and the volunteers started the second phase of this study. After erosive challenges, microhardness measurements were performed. Regardless of the restorative material employed, eroded specimens demonstrated lower microhardness value (p < 0.0001). At eroded condition examined in this study, dentine restored with glass‐ionomer cement showed higher microhardness values (p < 0.0001). It may be concluded that the glass‐ionomer cement decreases the progression of root dentine erosion at restoration margin. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Evaluation of the biocompatibility of novel peptide hydrogel in rabbit eye
Authors: Liang Liang , Xu Xiao‐Ding , Chen Chang‐Sheng , Fang Jia‐Hua , Jiang Fa‐Gang , Zhang Xian‐Zheng , Zhuo Ren‐Xi 
Published: March 2010

AbstractA peptide containing a RGD (arginine‐glycine‐aspartic acid) sequence as well as a hydrophobic N‐fluorenyl‐9‐methoxycarbonyl (FMOC) tail was prepared via a standard FMOC solid‐phase peptide synthesis technique. The supramolecular self‐assembly of such peptide through π–π stacking from FMOC tail can transfer the peptide aqueous solution into a three‐dimensional hydrogel. The biocompatibility of the peptide hydrogel was evaluated via clinical follow‐up and histological analysis. The data obtained demonstrated that the peptide hydrogel exhibited good biocompatibility when injected to the subconjunctival space and anterior chamber of rabbit, indicating a potential application in ophthalmology as an implantable drug delivery system for the treatment to ocular anterior segment diseases such as glaucoma, iridocyclitis, and keratopathy. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Bone repair by cell‐seeded 3D‐bioplotted composite scaffolds made of collagen treated tricalciumphosphate or tricalciumphosphate‐chitosan‐collagen hydrogel or PLGA in ovine critical‐sized calvarial defects
Authors: Haberstroh Kathrin , Ritter Kathrin , Kuschnierz Jens , Bormann Kai‐Hendrik , Kaps Christian , Carvalho Carlos , Mülhaupt Rolf , Sittinger Michael , Gellrich Nils‐Claudius 
Published: March 2010

AbstractThe aim of this study was to investigate the osteogenic effect of three different cell‐seeded 3D‐bioplotted scaffolds in a ovine calvarial critical‐size defect model. The choice of scaffold‐materials was based on their applicability for 3D‐bioplotting and respective possibility to produce tailor‐made scaffolds for the use in cranio‐facial surgery for the replacement of complex shaped boneparts. Scaffold raw‐materials are known to be osteoinductive when being cell‐seeded [poly(L‐lactide‐co‐glycolide) (PLGA)] or having components with osteoinductive properties as tricalciumphosphate (TCP) or collagen (Col) or chitosan. The scaffold‐materials PLGA, TCP/Col, and HYDR (TCP/Col/chitosan) were cell‐seeded with osteoblast‐like cells whether gained from bone (OLB) or from periost (OLP). In a prospective and randomized design nine sheep underwent osteotomy to create four critical‐sized calvarial defects. Three animals each were assigned to the HYDR‐, the TCP/Col‐, or the PLGA‐group. In each animal, one defect was treated with a cell‐free, an OLB‐ or OLP‐seeded group‐specific scaffold, respectively. The fourth defect remained untreated as control (UD). Fourteen weeks later, animals were euthanized for histo‐morphometrical analysis of the defect healing. OLB‐ and OLP‐seeded HYDR and OLB‐seeded TCP/Col scaffolds significantly increased the amount of newly formed bone (NFB) at the defect bottom and OLP‐seeded HYDR also within the scaffold area, whereas PLGA‐scaffolds showed lower rates. The relative density of NFB was markedly higher in the HYDR/OLB group compared to the corresponding PLGA group. TCP/Col had good stiffness to prepare complex structures by bioplotting but HYDR and PLGA were very soft. HYDR showed appropriate biodegradation, TCP/Col and PLGA seemed to be nearly undegraded after 14 weeks. 3D‐bioplotted, cell‐seeded HYDR and TCP/Col scaffolds increased the amount of NFB within ovine critical‐size calvarial defects, but stiffness, respectively, biodegradation of materials is not appropriate for the application in cranio‐facial surgery and have to be improved further by modifications of the manufacturing process or their material composition. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010
Bioactive inorganic‐materials/alginate composite microspheres with controllable drug‐delivery ability
Authors: Wu Chengtie , Zhu Yufang , Chang Jiang , Zhang Yufeng , Xiao Yin 
Published: March 2010

AbstractAlginate microspheres are considered a promising material as a drug carrier in bone repair because of excellent biocompatibility, but their main disadvantage is low drug entrapment efficiency and noncontrollable release. The aim of this study was to investigate the effect of incorporating mesoporous bioglass (MBG), nonmesoporous bioglass (BG), or hydroxyapatite (HAp) into alginate microspheres on their drug‐loading and release properties. X‐ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and atomic emission spectroscopy (AES) were used to analyze the composition, structure, and dissolution of bioactive inorganic materials and their microspheres. Dexamethasone (DEX)‐loading and release ability of four microspheres were tested in phosphate buffered saline with varying pH. Results showed that the drug‐loading capacity was enhanced with the incorporation of bioactive inorganic materials into alginate microspheres. The MBG/alginate microspheres had the highest drug loading ability. DEX release from alginate microspheres correlated to the dissolution of MBG, BG, and HAp in PBS, and that the pH was an efficient factor in controlling the DEX release; a high pH resulted in greater DEX release, whereas a low pH delayed DEX release. In addition, MBG/alginate, BG/alginate, and HAp/alginate microspheres had varying apatite‐formation and dissolution abilities, which indicate that the composites would behave differently with respect to bioactivity. The study suggests that microspheres made of a composite of bioactive inorganic materials and alginate have a bioactivity and degradation profile which greatly improves their drug delivery capacity, thus enhancing their potential applications as bioactive filler materials for bone tissue regeneration. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2010.
The T-13910C polymorphism in the lactase phlorizin hydrolase gene is associated with differences in serum calcium levels and calcium intake
Authors: Koek W Nadia H , van Meurs Joyce B , van der Eerden Bram CJ , Rivadeneira Fernando , Zillikens M Carola , Hofman Albert , Obermayer-Pietsch Barbara , Lips Paul , Pols Huibert A , Uitterlinden André G , van Leeuwen Johannes PTM 
Published: March 2010

Abstract
The C-variant of a T-13910C polymorphism (rs4988235; NT_022135.15:g.25316568G &gt; A) upstream of the lactase phlorizin hydrolase (LPH) gene causes lactose intolerance. Association studies with differences in bone parameters and fracture risk have been inconclusive. The objective of this study was to examine the association of LPH rs4988235 with body height and bone parameters and calcium homeostasis in two elderly populations of Dutch Caucasians and assess interaction with vitamin D receptor (VDR) polymorphisms. Genotyping of LPH and VDR polymorphisms was performed in 6367 individuals from the Rotterdam Study and 844 from the Longitudinal Aging Study Amsterdam (LASA). Associations with age, height, weight, bone mineral density (BMD), skeletal morphometric parameters and serum vitamin D and calcium levels, and dietary calcium intake were assessed using ANOVA or analysis of covariance, and allele dose effect was assessed using linear regression analysis. Fracture risk was analyzed using Cox's proportional hazard regression analysis. Associations with body height (p = 2.7 × 10−8) and vertebral area (p = .048) found in the Rotterdam Study were explained by population stratification, as assessed by principal-component analyses, and disappeared after additional adjustments. No associations with femoral neck or lumbar spine BMD or with fracture risk were detected. Calcium intake and serum ionized serum calcium were significantly lower in C-homozygotes (p = 9.2 × 10−7, p = .02, respectively). For none of the parameters studied was interaction between the T-13910C polymorphism and VDR block 5 haplotype 1 observed. We show that the C allele of the T-13910C polymorphism causing lactose intolerance is associated with lower dietary calcium intake and serum calcium levels but not with BMD or fractures. The associations observed with height and vertebral area were the result of population stratification. This demonstrates the impact of population stratification and urges researchers to carefully take this into account in genetic associations, in particular, in dietary intake–related phenotypes, of which LPH and lactose intolerance are a strong example. © 2010 American Society for Bone and Mineral Research
Src‐like adaptor protein regulates osteoclast generation and survival
Authors: Kim Hyun‐Ju , Zou Wei , Ito Yuji , Kim Shin‐Yoon , Chappel Jean , Ross F. Patrick , Teitelbaum Steven L. 
Published: March 2010

AbstractSrc‐like adaptor protein (SLAP) is a hematopoietic adaptor containing Src homology (SH)3 and SH2 motifs and a unique carboxy terminus. Unlike c‐Src, SLAP lacks a tyrosine kinase domain. We investigated the role of SLAP in osteoclast development and resorptive function. Employing SLAP‐deficient mice, we find lack of the adaptor enhances in vitro proliferation of osteoclast precursors in the form of bone marrow macrophages (BMMs), without altering their survival. Furthermore, osteoclastogenic markers appear more rapidly in SLAP−/− BMMs exposed to RANK ligand (RANKL). The accelerated proliferation of M‐CSF‐treated, SLAP‐deficient precursors is associated with enhanced ERK activation. SLAP's role as a mediator of M‐CSF signaling, in osteoclastic cells, is buttressed by complexing of the adaptor protein and c‐Fms in lipid rafts. Unlike c‐Src, SLAP does not impact resorptive function of mature osteoclasts but induces their early apoptosis. Thus, SLAP negatively regulates differentiation of osteoclasts and proliferation of their precursors. Conversely, SLAP decreases osteoclast death by inhibiting activation of caspase 3. These counterbalancing events yield indistinguishable bones of WT and SLAP−/− mice which contain equal numbers of osteoclasts in basal and stimulated conditions. J. Cell. Biochem. 110: 201–209, 2010. © 2010 Wiley‐Liss, Inc.
Dioscorealide B suppresses LPS‐induced nitric oxide production and inflammatory cytokine expression in RAW 264.7 macrophages: The inhibition of NF‐κB and ERK1/2 activation
Authors: Hiransai Poonsit , Ratanachaiyavong Suvina , Itharat Arunporn , Graidist Potchanapond , Ruengrairatanaroj Prasit , Purintrapiban Juntipa 
Published: March 2010

AbstractDioscorealide B (DB), a naphthofuranoxepin has been purified from an ethanolic extract of the rhizome of Dioscorea membranacea Pierre ex Prain & Burkill which has been used to treat inflammation and cancer in Thai Traditional Medicine. Previously, DB has been reported to have anti‐inflammatory activities through reducing nitric oxide (NO) and tumor necrosis factor‐α (TNF‐α) production in lipopolysaccharides (LPS)‐induced RAW 264.7 macrophage cells. In this study, the mechanisms of DB on LPS‐induced NO production and cytokine expression through the activation of nuclear factor‐κB (NF‐κB) and ERK1/2 are demonstrated in RAW 264.7 cells. Through measurement with Griess's reagent, DB reduced NO level with an IC50 value of 2.85 ± 0.62 µM that was due to the significant suppression of LPS‐induced iNOS mRNA expression as well as IL‐1β, IL‐6, and IL‐10 mRNA at a concentration of 6 µM. At the signal transduction level, DB significantly inhibited NF‐κB binding activity, as determined using pNFκB‐Luciferase reporter system, which action resulted from the prevention of IκBα degradation. In addition, DB in the range of 1.5–6 µM significantly suppressed the activation of the ERK1/2 protein. In conclusion, the molecular mechanisms of DB on the inhibition of NO production and mRNA expression of iNOS, IL‐1β, IL‐6, and IL‐10 were due to the inhibition of the upstream kinases activation, which further alleviated the NF‐κB and MAPK/ERK signaling pathway in LPS‐induced RAW264.7 macrophage cells. J. Cell. Biochem. 109: 1057–1063, 2010. © 2010 Wiley‐Liss, Inc.
The role of the cysteine residue in the chaperone and anti‐apoptotic functions of human Hsp27
Authors: Pasupuleti NagaRekha , Gangadhariah Mahesha , Padmanabha Smitha , Santhoshkumar Puttur , Nagaraj Ram H. 
Published: March 2010

AbstractThe small heat shock protein Hsp27 is a molecular chaperone and an anti‐apoptotic protein. Human Hsp27 has one cysteine residue at position 137. We investigated the role of this cysteine residue in the chaperone and anti‐apoptotic functions of Hsp27 by mutating the cysteine residue to an alanine (Hsp27C137A) and comparing it to wild‐type protein (Hsp27WT). Both proteins were multi‐subunit oligomers, but subunits of Hsp27WT were disulfide‐linked unlike those of Hsp27C137A, which were monomeric. Hsp27C137A was indistinguishable from Hsp27WT with regard to its secondary structure, surface hydrophobicity, oligomeric size and chaperone function. S‐thiolation and reductive methylation of the cysteine residue had no apparent effect on the chaperone function of Hsp27WT. The anti‐apoptotic function of Hsp27C137A and Hsp27WT was studied by overexpressing them in CHO cells. No difference in the caspase‐3 or ‐9 activity was observed in staurosporine‐treated cells. The rate of apoptosis between Hsp27C137A and Hsp27WT overexpressing cells was similar whether the cells were treated with staurosporine or etoposide. However, the mutant protein was less protective relative to the wild‐type protein in preventing caspase‐3 and caspase‐9 activation and apoptosis induced by 1 mM H2O2 in CHO and HeLa cells. These data demonstrate that in human Hsp27, disulfide formation by the lone cysteine does not affect its chaperone function and anti‐apoptotic function against chemical toxicants. However, oxidation of the lone cysteine in Hsp27 might at least partially affect the anti‐apoptotic function against oxidative stress. J. Cell. Biochem. 110: 408–419, 2010. © 2010 Wiley‐Liss, Inc.
Rottlerin inhibits migration of follicular thyroid carcinoma cells by PKCδ‐independent destabilization of the focal adhesion complex
Authors: Lin Chien‐Jung , Lin Chieh‐Yu , Chen Ying , Huang Shih‐Horng , Wang Seu‐Mei 
Published: March 2010

AbstractThis study examined the effect of rottlerin on the focal adhesion‐mediated cell migration of CGTH W‐2 human follicular thyroid carcinoma cells. Rottlerin (10 µM) resulted in decreased adhesion of CGTH W‐2 cells to matrix substance, which was correlated with metastatic potential. Rottlerin treatment also resulted in a marked reduction in the migration of CGTH W‐2 cells. Protein levels of integrin β1, FAK, and paxillin were decreased by rottlerin. Consistent with this, immunostaining of FAK, vinculin, and paxillin revealed disassembly of the focal adhesions. Disruption of actin stress fibers was noted, which was compatible with reduced expression levels and activities of Rac‐1 and Rho. The effect of rottlerin on cell migration was not attributable to inhibition of PKCδ activity since siRNA knockdown of PKCδ did not recapitulate the effects of rottlerin on cell adhesion and migration. Furthermore, activation of PKCδ by phorbol esters failed to restore the rottlerin‐inhibited migratory ability. The mitochondrial uncoupler, carbonylcyanide‐4‐(trifluoromethoxy)‐phenylhydrazone, was able to mimic several rottlerin's effects. In summary, we demonstrated that rottlerin inhibits the migration of CGTH W‐2 cells by disassembly of focal adhesion complexes in a PKCδ‐independent manner, and might play as a mitochondrial uncoupler role in these events. J. Cell. Biochem. 110: 428–437, 2010. © 2010 Wiley‐Liss, Inc.
CCAAT/enhancer binding protein beta is up‐regulated in giant cell tumor of bone and regulates RANKL expression
Authors: Ng Patrick Kwok‐Shing , Tsui Stephen Kwok‐Wing , Lau Carol Po‐Ying , Wong Chi‐Hang , Wong Winnie Hiu‐Ting , Huang Lin , Kumta Shekhar‐Madhukar 
Published: March 2010

AbstractGiant cell tumor (GCT) of bone is an aggressive non‐cancerous tumor, which consists of multi‐nucleated osteoclast‐like giant cells, stromal cells, and monocytes. It is believed that stromal cells are the neoplastic component of this tumor. Expression of the receptor activator of nuclear factor kappa B ligand (RANKL) in the stromal cells stimulates the monocytes to form giant multi‐nucleated osteoclast‐like cells, causing bone over‐resorption at the tumor site. Previously, our group has reported the up‐regulation of RANKL in GCT of bone stromal cells, but the mechanism is unknown. Using stromal cell culture of GCT obtained from patients, we demonstrated the up‐regulation of the transcriptional activator CCAAT/enhancer binding protein beta (C/EBPβ). RANKL promoter studies revealed that C/EBPβ over‐expression induced RANKL promoter activity in a dose‐dependent manner and a CCAAT‐box within the region nt −357/−1 contributed to the basal transcription activity, with a possible C/EBPβ binding element in the region nt −460/−358 leading to further induction. Furthermore, we also showed that C/EBPβ bound to the RANKL promoter in GCT stromal cells in vivo by chromatin immunoprecipitation. To conclude, our study has shown that C/EBPβ is a RANKL promoter activator in stromal cells of GCT of bone and we have proposed a model in which C/EBPβ plays an important role in the osteolytic characteristics and pathological causes of GCT of bone. J. Cell. Biochem. 110: 438–446, 2010. © 2010 Wiley‐Liss, Inc.
The hydro‐meteorological estimation of probable maximum precipitation under varying scenarios in Sri Lanka
Authors: Fernando W. C. D. K. , Wickramasuriya S. S. 
Published: March 2010

AbstractProbable maximum precipitation (PMP) is widely used as an input in rainfall‐runoff modelling to estimate probable maximum flood (PMF), which is required for the design of spillways of large dams. In relation to dam safety studies at 32 dam sites, an analysis was done of extreme rainfalls in Sri Lanka, which is influenced by the Asian monsoon system and cyclonic effects. Accordingly, the Hershfield's statistical method, which is one of the recommended procedures of the World Meteorological Organization (WMO), was used for estimating PMP. It is also a technique that can be readily used in design office practice. However, it is of significant value and scientific interest to explore what the outcome would have been if the physically based hydro‐meteorological procedure had been adopted. For this purpose, long series of annual maximum daily rainfall data from seven stations were analysed. The research shows that the maximum moisture and corresponding wind run or the use of maximum moisture alone are two scenarios, which yield results compatible with the statistical PMP. Although globally there is a tendency to exclude wind maximization, this does not automatically apply to a country like Sri Lanka, which experiences cyclonic rainfall and special attention should be paid to precipitation efficiency. These results are further confirmed by a frequency analysis based on the generalized extreme value (GEV) distribution. An approximate value of PMP could be in the order of 2.0–3.0 times the maximum observed daily rainfall. However, this aspect needs further investigation when observed maxima are near PMP storms. Copyright © 2010 Royal Meteorological Society