bluuebunny/crossref_metadata_2025_split huggingface dataset:
Epigenetic down regulation of RASSF10 and its possible clinical implication in prostate carcinoma
Authors: Dansranjavin Temuujin , Wagenlehner Florian , Gattenloehner Stefan , Steger Klaus , Weidner Wolfgang , Dammann Reinhard , Schagdarsurengin Undraga 
Published: March 2012

AbstractBACKGROUNDRas association domain family (RASSF) comprises several tumor suppressor genes, which are often epigenetically inactivated in human tumors. Here, we aim to analyze the relevance of the recently identified member RASSF10 in prostate carcinogenesis.METHODSRASSF10 promoter methylation and mRNA expression were investigated by bisulfite‐pyrosequencing and qRT‐PCR, respectively, in prostate carcinoma (PCa) cell lines (LNCaP, 22Rv1, DU‐145) and in 83 primary PCa and 53 primary benign prostatic hyperplasia (BPH) tissues obtained after radical prostatectomy. Histological localization of RASSF10 was done by in situ hybridization. To prove the epigenetic nature of RASSF10 down regulation, PCa cell lines were treated with 5‐aza‐2‐deoxycytidine and trichostatin A. Potential function of RASSF10 was analyzed in LNCaP by colony formation and apoptosis assays.RESULTSRASSF10 mRNA was localized to cells of the basal layer of the prostatic gland. Absence (LNCaP) and decrease (22Rv1, DU‐145) of RASSF10 expression was associated with promoter methylation and could be restored by demethylating agents. A link between RASSF10 mRNA reduction and promoter methylation was also detected in primary prostate tissues (P = 0.006), where PCa showed more frequently reduced RASSF10 levels when compared with BPH (33.7% vs. 13.2%, P = 0.009). RASSF10 methylation could be further associated with advanced tumor stage and advanced age (P‐values < 0.05). Our preliminary functional assays revealed the ability of RASSF10 to inhibit colony formation (P = 0.018) and to increase apoptosis (P = 0.035).CONCLUSIONSThis is the first study, which demonstrates the frequent epigenetic inactivation of RASSF10 in PCa and its implication in clinical symptoms of PCa. Prostate 72:1550–1558, 2012. © 2012 Wiley Periodicals, Inc.
Mimicking the action of folding chaperones by Hamiltonian replica‐exchange molecular dynamics simulations: Application in the refinement of de novo models
Authors: Fan Hao , Periole Xavier , Mark Alan E. 
Published: March 2012

AbstractThe efficiency of using a variant of Hamiltonian replica‐exchange molecular dynamics (Chaperone H‐replica‐exchange molecular dynamics [CH‐REMD]) for the refinement of protein structural models generated de novo is investigated. In CH‐REMD, the interaction between the protein and its environment, specifically, the electrostatic interaction between the protein and the solvating water, is varied leading to cycles of partial unfolding and refolding mimicking some aspects of folding chaperones. In 10 of the 15 cases examined, the CH‐REMD approach sampled structures in which the root‐mean‐square deviation (RMSD) of secondary structure elements (SSE‐RMSD) with respect to the experimental structure was more than 1.0 Å lower than the initial de novo model. In 14 of the 15 cases, the improvement was more than 0.5 Å. The ability of three different statistical potentials to identify near‐native conformations was also examined. Little correlation between the SSE‐RMSD of the sampled structures with respect to the experimental structure and any of the scoring functions tested was found. The most effective scoring function tested was the DFIRE potential. Using the DFIRE potential, the SSE‐RMSD of the best scoring structures was on average 0.3 Å lower than the initial model. Overall the work demonstrates that targeted enhanced‐sampling techniques such as CH‐REMD can lead to the systematic refinement of protein structural models generated de novo but that improved potentials for the identification of near‐native structures are still needed. Proteins 2012; © 2012 Wiley Periodicals, Inc.
Growth and characterization of self‐assembled low‐indium composition InGaN nanodots by alternate admittance of precursors
Authors: Zhao Wei , Wang Lai , Wang Jiaxing , Lv Wenbin , Hao Zhibiao , Luo Yi 
Published: March 2012

AbstractLow‐indium composition InGaN nanodots are grown on GaN/Al2O3 by alternate admittance of triethylgallium (TEGa), trimethylindium (TMIn), and ammonia (NH3) using metalorganic vapor phase epitaxy. Since the lattice mismatch between metal (Ga, In) and GaN is large during the admittance of group III precursors and the alternate admittance of precursors can enhance the surface diffusion of adatoms, it is believed that the growth mode of InGaN nanodots is the Volmer–Weber mode. The admittance time is optimized and injecting TMIn with NH3 can increase indium composition. The ultraviolet photoluminescence spectra exhibit small temperature‐dependent redshift and a sensitive intensity change dependent on indium composition.
Synthesis of a new donor, MOET‐TTP, and the crystal structure, electrical, and magnetic properties of (MOET‐TTP)2MCl4 (M = Fe and Ga)
Authors: Shirahata Takashi , Ito Tetsuya , Toki Shinya , Misaki Yohji 
Published: March 2012

AbstractA new 2,5‐bis(1,3‐dithiol‐2‐ylidene)‐1,3,4,6‐tetrathiapentalene (BDT‐TTP) type donor, 2‐(4,5‐dimethoxy‐1,3‐dithiol‐2‐ylidene)‐5‐(4,5‐ethylenedithio‐1,3‐dithiol‐2‐ylidene)‐1,3,4,6‐tetrathiapentalene (MOET‐TTP) was synthesized and its magnetic and non‐magnetic anion salts (MOET‐TTP)2MCl4 (M = Fe and Ga), were prepared. These salts are isostructural and belong to the space group of P1. They show metal‐like behavior down to 200 K for the FeCl4 salt and 20 K for the GaCl4 salt. The band structures of these salts are two‐dimensional. The MCl4 anions are located between the donor columns and close to the ethylenedithio and methoxy groups of the donor molecules. The magnetic susceptibilities of the FeCl4 salts follow the Curie–Weiss law with Curie constant of 4.3 emu K mol−1 and Weiss temperature of θ = −1.8 K, revealing a weak antiferromagnetic interaction of 3d spins of the FeCl4 anions. The ${\rm Fe}\cdots {\rm Fe}$ (6.508(3) Å) and ${\rm Cl}\cdots {\rm Cl}$ (3.78–4.69 Å) distances in the crystal structure of the salt are significantly long. Therefore, the direct magnetic interaction between the 3d spins of the nearest neighboring Fe3+ ions does not seem readily accessible.
Properties of crystalline coronene: Dispersion forces leading to a larger van der Waals radius for carbon
Authors: Fedorov Igor , Zhuravlev Yurii , Berveno Victor 
Published: March 2012

AbstractWe investigated the electronic structure of crystalline coronene within the framework of the dispersion corrected density‐functional theory (DFT‐D). We use the DFT‐D2 and DFT‐D3 methods. Utilization of the DFT‐D3 leads to a correct account of the dispersion forces, which results in a good agreement of the computed lattice parameters and cohesive energy. In the framework of DFT‐D3 the cohesive energy of crystalline coronene is 1.483 eV. Computed lattice constants and cohesive energy show good agreement with experimental data if larger van der Waals (vdW) radius for carbon is used. Based on the relaxed crystal structures, we have computed the band structure, total and deformation electron density.
Properties of nitride‐based photovoltaic cells under concentrated light illumination
Authors: Yamamoto Shota , Mori Mikiko , Kuwahara Yosuke , Fujii Takahiro , Nakao Tatsuo , Kondo Shinichiro , Iwaya Motoaki , Takeuchi Tetsuya , Kamiyama Satoshi , Akasaki Isamu , Amano Hiroshi 
Published: March 2012

AbstractWe investigated the properties of nitride‐based solar cells under concentrated light illumination from 1 to 200 suns. The conversion efficiency of our solar cells increased with increasing concentration up to 200 suns. The short‐circuit cur‐ rent density, open‐circuit voltage, fill factor, and conversion efficiency were 510 mA/cm2, 1.9 V, 70%, and 3.4%, respectively, under an air mass filter of 1.5G at 200 suns and room temperature. (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
From deflection function to potential energy: A Firsov approach critical analysis
Authors: Alves Márcio Oliveira , Oliveira Jessé Moreira , Lemes Nelson Henrique Teixeira , Braga João Pedro 
Published: March 2012

AbstractThe Firsov inverse method was used to retrieve potential energy for helium–helium system from deflection function. Using a combination of accurate simulated data for large deflection function and a Lennard–Jones type potential for smaller values, it was possible to recover the short‐range potential in excellent agreement with the theoretical results. Errors in the deflection function ranged from 1 to 10% with a collision energy from 2 × 10−3 to 2 eV. Inverted potential were obtained with a precision from 2 to 8%. This study explores the possibility to use Firsov approach for small collision energy, unlike the previous work on the subject. The method was proved to be robust, stable against experimental error, and very easy to be implemented numerically. © 2012 Wiley Periodicals, Inc.
Atoms‐in‐molecules analysis of the effect of intermolecular interactions on dielectric properties in hydrogen‐bonded material 5‐bromo‐9‐hydroxyphenalenone
Authors: Otaki Hiroki , Ando Koji 
Published: March 2012

AbstractIntermolecular interactions in molecular crystal of 5‐bromo‐9‐hydroxyphenalenone are analyzed by means of Bader's theory of atoms in molecules. A set of criteria to ascertain the presence of a hydrogen bond is applied to two candidates of intermolecular contacts suggested by our previous work [Otaki and Ando,Phys. Chem. Chem. Phys.2011,13, 10719]. It is shown that they almost satisfy the criteria to confirm the existence of intermolecular CH···O hydrogen bond. In addition to the hydrogen bonding, other types of interactions, such as H···H and H···Br, are found in one of the candidates. The discussions are extended to explain how the molecular dipole moment is induced by surrounding molecules. It is also found that the bias in the atomic charges due to the electrophilicity of the oxygen atom is strongly correlated with the induced dipole moment. © 2012 Wiley Periodicals, Inc.
Identification of Risk Factors for Esophagojejunal Anastomotic Leakage after Gastric Surgery
Authors: Deguchi Yasunori , Fukagawa Takeo , Morita Shinji , Ohashi Masaki , Saka Makoto , Katai Hitoshi 
Published: March 2012

AbstractBackgroundDespite the development of the surgical technique and improvements in perioperative management, anastomotic leakage still occurs at esophagojejunal anastomoses after total or proximal gastrectomy. Anastomotic leakage is one of the major complications of concern, chiefly because it can lead to death. The objective of the present study was to identify the risk factors for esophagojejunal anastomotic leakage.MethodsThe study was based on retrospective analysis of the data of a total of 1,640 consecutive patients who underwent total, proximal, or completion gastrectomy, including esophagojejunal anastomosis, between 1999 and 2008.ResultsThirty‐five patients (2.1 %) developed anastomotic leakage. Univariate analysis revealed patient age, pulmonary insufficiency, lymph node dissection, combined resection of other organs, omental resection, operative time, blood loss, intraoperative blood transfusion, and postoperative creatinine level were the significant factors influencing anastomotic leakage. Multivariate analysis identified pulmonary insufficiency and the duration of the operation as the predictors of anastomotic leakage.ConclusionsTo avoid leakage, surgeons should take care in creating the anastomosis in gastrectomy patients, particularly in cases of poor pulmonary function or when the procedure requires a longer operation.
Oral Supplementation with Dihomo‐γ‐Linolenic Acid (DGLA)‐Enriched Oil Increases Serum DGLA Content in Healthy Adults
Authors: Tanaka Takao , Kakutani Saki , Horikawa Chika , Kawashima Hiroshi , Kiso Yoshinobu 
Published: March 2012

AbstractDihomo‐γ‐linolenic acid (DGLA) is one of the polyunsaturated fatty acids, and is expected to show anti‐allergic activity. We examined the effects of supplementation with DGLA‐enriched oil (450 mg as free DGLA) for 4 weeks in healthy adults in a randomized controlled study. The DGLA composition in the total fatty acids of serum phospholipids increased from 2.0 to 3.4 %, and returned to the initial level after a 4‐week washout. No side effects or changes in blood biochemical parameters were observed. These results indicate that serum DGLA content can be safely increased by supplementation with 450 mg DGLA under these conditions.
Kinetic Study of Hydroperoxide Degradation in Edible Oils Using Electron Spin Resonance Spectroscopy
Authors: Roman Olesea , Courtois Francis , Maillard Marie‐Noëlle , Riquet Anne‐Marie 
Published: March 2012

AbstractLipid oxidation is a complex phenomenon involving free radicals which are highly reactive molecular species. The life‐time of these radical species is extremely short and their detection is therefore difficult. Several electron spin resonance (ESR) spectroscopy methodologies make it possible to identify, quantify and measure the reactivity of radical species formed during oxidation–reduction reactions. In this study we took advantage of the specificity of ESR spectroscopy to detect radical compounds in order to determine the rate constants of hydroperoxide degradation, a key reaction involved in lipid oxidation. The interaction of 5‐doxyl stearic acid and lipid‐derived radicals was studied by following the intensity of ESR spectra. A kinetic model was developed to simulate data analysis obtained by ESR and values of rate constants for hydroperoxide degradation were determined at 100 and 110 °C. This quantitative approach of ESR spectroscopy has produced useful information about new rate estimates for hydroperoxide degradation in edible oils.
Explaining educational differences in adolescent substance use and early sexual debut: The role of parents and peers
Authors: de Looze Margaretha , Harakeh Zeena , van Dorsselaer Saskia A.F.M. , Raaijmakers Quinten A.W. , Vollebergh Wilma A.M. , ter Bogt Tom F.M. 
Published: March 2012

ABSTRACTPrevious studies from a wide variety of European countries have demonstrated that low educated adolescents engage more frequently in health risk behaviors compared to high educated adolescents. The present study investigates the mediating roles of parental knowledge and time spent with peers in this relationship. Data were retrieved from a nationally representative sample of 12‐ to 16‐year old Dutch adolescents (N = 5422). Risk behaviors were measured by adolescents’ report of daily smoking, binge drinking and cannabis use in the previous month, and sexual debut before age 17. Low educated adolescents indicated that their parents had less knowledge on their whereabouts and reported spending more time with peers than high educated adolescents. Both factors mediated the relationship with health risk behaviors. These results hint to parenting practices and adolescent peer relations as points of reference for prevention and intervention work aiming to reduce educational inequalities in adolescent health risk behaviors.
Cold-water corals landed by bottom longline fisheries in the Azores (north-eastern Atlantic)
Authors: Sampaio Í. , Braga-Henriques A. , Pham C. , Ocaña O. , de Matos V. , Morato T. , Porteiro F.M. 
Published: March 2012

The impact of bottom trawling on cold-water corals (CWC) has been thoroughly studied and shown to be long-lasting; however the effects of bottom longlining on CWC ecosystems have received little attention. The present paper identifies the principal CWC species landed by bottom longlining in Faial (Azores) from 150 to 600 m depth. Data were obtained from a survey of 297 landings during four months coupled with 16 interviews with fishermen. A distinction was made among corals brought on deck directly entangled in the fishing gear (primary by-catch) from corals brought up associated with other larger CWC species or rocks (secondary by-catch). Forty-five (15.2%) of 297 fishing trips surveyed landed coral specimens. The survey recorded 39 different CWC taxa in the by-catch, belonging to five different orders (Scleractinia, Alcyonacea, Antipatharia, Zoanthidea and Anthoathecata). Secondary by-catch included a larger number of species but the total number of corals was in the same order of magnitude for both groups. The taxa most frequently encountered wereLeiopathesspp., Errina dabneyiand Dendrophyllia sp. CWC taxa in the by-catch were mostly medium size (10–60 cm), 3-dimensional and branched colonies. Local ecological knowledge of fishermen confirmed that the corals recorded were representative of their past experience and also revealed a general agreement that there has been a decrease of CWC by-catch on traditional fishing grounds. Corals are common by-catch in bottom longline fisheries around the Azores and so conservation measures may be required.
Effects of CH foam preplasma on fast ignition
Authors: Hata M. , Sakagami H. , Sunahara A. , Johzaki T. , Nagatomo H. 
Published: March 2012

AbstractCreation of a preformed plasma (preplasma) by heating laser prepulse is crucial to fast ignition. Because it is difficult to control the prepulse and preplasma, control of fast electron beam generation by low-density foam was recently reported. However, this simulation study ignored the foam preplasma. Therefore, we calculated foam preplasma formation using a hydrodynamic code and investigated the effects of the preplasma on fast ignition by using integrated simulations, including radiation hydrodynamic, Particle-In-Cell (PIC) and Fokker–Planck simulations. We conclude that the average core temperature decreased by approximately 10% in the integrated simulations of the foam preplasma case.
Effects of dielectric discontinuity on the dispersion characteristics of the tape helix slow-wave structure with two metal shields
Authors: Zhang Yu , Liu Jinliang , Wang Shiwen , Fan Xuliang , Zhang Hongbo , Feng Jiahuai 
Published: March 2012

AbstractIn the tape helix slow-wave system, discontinuous dielectrics have great effects on the dispersion characteristics. In this paper, the tape helix slow-wave system, including an inner and an outer metal shield, tape helix, nylon support, and de-ionized water as filling dielectric, was analyzed. Effects of dielectric discontinuity caused by the support dielectric and filling dielectric on the dispersion characteristics were studied in detail. The dispersion relations, phase velocities, slow-wave coefficients, and electric lengths of the spatial harmonics in the system were calculated. Results showed that, if the permittivity of support dielectric was smaller than that of the filling dielectric, frequencies of the spatial harmonics in the system rose, phase velocities and slow-wave coefficients increased, the slow-wave effect of the system was weakened so that the previous electric length was shortened. The reverse condition corresponded to the reverse results, and the electromagnetic simulation also proved it. By use of the helical pulse forming line of accelerator based on the studied tape helix slow-wave system, the electric lengths of the system were tested as 188.5 ns and 200 ns in experiments where the thicknesses of nylon support were 6 mm and 3 mm, respectively. The theoretical calculation results 198 ns and 211 ns basically corresponded to experimental results, which only had relative errors as 5 and 5.5%, respectively.
Modified wire array underwater electrical explosion
Authors: Gilburd L. , Efimov S. , Fedotov Gefen A. , Gurovich V. Tz. , Bazalitski G. , Antonov O. , Krasik Ya. E. 
Published: March 2012

AbstractThe results of experiments involving underwater electrical explosion of different wire arrays using an outer metallic cylinder as a shock reflector are presented. A pulse generator with a stored energy of about 6 kJ, current amplitude ≤ 500 kA, and rise time of 350 ns was used for the wire array explosion. The results of the experiments and of hydrodynamic simulations showed that in the case of a Cu wire array explosion, the addition of the reflector increases the pressure and temperature of the water in the vicinity of the implosion axis about 1.38 and about 1.33 times, respectively. Also, it was shown that in the case of an Al wire array explosion with stainless steel reflector, Al combustion results, and, accordingly, additional energy is delivered to the converging water flow generating about 540 GPa pressure in the vicinity of the explosion axis. Finally, it was found that microsecond time scale light emission that appears with microsecond time scale delay with respect to the nanosecond time scale self-light emission of the compressed water in the vicinity of the implosion axis is related to water bubbles formation which scattered light of exploded wires.
Transnational Access to Environmental Information
Authors: Krämer Ludwig 
Published: March 2012

AbstractThis paper traces back the efforts, in particular in Europe, to promote transnational legal provisions which grant a right of access to environmental information. Initiatives in the 1970s failed to establish a fundamental right to a clean environment. However, the establishment of fundamental procedural rights of access to information, participation in decision-making and access to justice in environmental matters has been more successful – culminating in the 1998 Aarhus Convention. This paper describes the – until now unsuccessful – attempts to extend the territorial scope of application of the Aarhus Convention to non-European countries and regions, and ultimately the conclusion of a global convention on access to environmental information.
The Effect of Articulatory Adjustment on Reducing Hypernasality
Authors: Rong Panying , Kuehn David 
Published: March 2012

Purpose
With the goal of using articulatory adjustments to reduce hypernasality, this study utilized an articulatory synthesis model (Childers, 2000) to simulate the adjustment of articulatory configurations with an open velopharynx to achieve the same acoustic goal as normal speech simulated with a closed velopharynx.

Method
To examine the effect of articulatory adjustment on perceived nasality, this study used an articulatory synthesis model (Childers, 2000) to synthesize 18 oral /i/ vowels, 18 nasal /i/ vowels, and 18 nasal /i/ vowels with computer-generated articulatory adjustments; these vowels were then presented to 7 listeners for perceptual ratings of nasality following the direct magnitude estimation method.

Results
Comparisons of nasality ratings of nasal vowels showed a significant reduction of perceived nasality after articulatory adjustment. Moreover, the acoustic features associated with nasal resonances were attenuated and the oral formant structures changed by nasalization were restored after articulatory adjustment, which confirmed findings in Rong and Kuehn (2010).

Conclusion
Appropriate articulatory adjustments are able to reduce the nasality of synthetic nasal /i/ vowels by compensating for the acoustic deviations caused by excessive velopharyngeal opening. Such compensatory interarticulator coordination may have an application in using articulatory adjustments to reduce hypernasality in clinical speech therapies.