bluuebunny/crossref_metadata_2025_split huggingface dataset:
HLA-E and HLA-G Expression in Classical HLA Class I-Negative Tumors Is of Prognostic Value for Clinical Outcome of Early Breast Cancer Patients
Authors: de Kruijf Esther M. , Sajet Anita , van Nes Johanna G. H. , Natanov Russ , Putter Hein , Smit Vincent T. H. B. M. , Liefers Gerrit Jan , van den Elsen Peter J. , van de Velde Cornelis J. H. , Kuppen Peter J. K. 
Published: November 2010

Abstract
Nonclassical HLAs, HLA-E and HLA-G, are known to affect clinical outcome in various tumor types. We examined the clinical impact of HLA-E and HLA-G expression in early breast cancer patients, and related the results to tumor expression of classical HLA class I. Our study population (n = 677) consisted of all early breast cancer patients primarily treated with surgery in our center between 1985 and 1995. Tissue microarray sections of arrayed tumor and normal control material were immunohistochemically stained for HLA-E and HLA-G. For evaluation of HLA-E and HLA-G and the combined variable, HLA-EG, a binary score was used. Expression of classical HLA class I molecules was determined previously. HLA-E, HLA-G, and HLA-EG on breast tumors were classified as expression in 50, 60, and 23% of patients, respectively. Remarkably, only in patients with loss of classical HLA class I tumor expression, expression of HLA-E (p = 0.027), HLA-G (p = 0.035), or HLA-EG (p = 0.001) resulted in a worse relapse-free period. An interaction was found between classical and nonclassical HLA class I expression (p = 0.002), suggestive for a biological connection. We have demonstrated that, next to expression of classical HLA class I, expression of HLA-E and HLA-G is an important factor in the prediction of outcome of breast cancer patients. These results provide further evidence that breast cancer is immunogenic, but also capable of evading tumor eradication by the host’s immune system, by up- or downregulation of HLA class Ia and class Ib loci.
Antibacterial, spectral and thermal aspects of some novel first transition metal‐based heterochelates
Authors: Oza Kaushal K. , Jani Darshan H. , Patel Hasmukh S. 
Published: November 2010

AbstractA new ligand, 5‐{[5‐(pyridine‐4‐yl)‐1,3,4‐oxadiazole‐2‐ylthio]methyl}quinoline‐8‐ol (K), was synthesized by base‐catalysed reaction of 5‐chloromethyl‐8‐hydroxy quinoline (CMQ) and 5‐(pyridine‐4‐yl)‐1,3,4‐oxadiazole‐2‐thiol. The obtained ligand K was characterized by1H NMR,13C NMR and IR spectroscopic techniques and reacted with transition metal salts to afford metal‐containing heterochelates. The structures of the synthesized heterochelates were characterized using elemental analyses, infrared spectra, electronic spectra, magnetic measurements, FAB mass spectrum and thermogravimetric analyses. The kinetic parameters such as order of reaction (n) and the energy of activation (Ea) are reported using the Freeman–Carroll method. The pre‐exponential factor (A), the activation entropy (ΔS#), the activation enthalpy (ΔH#) and the free energy of activation (ΔG#) were calculated. Heterochelates were also screened for theirin vitroantibacterial activity against a range of Gram‐positive (Bacillus substilis, Staphylococcus aureus) and Gram‐negative (Escherichia coli, Serratiamarcescens) organisms. Copyright © 2010 John Wiley & Sons, Ltd.
Plastic microfluidic chip for continuous‐flow polymerase chain reaction: Simulations and experiments
Authors: Cao QingQing , Kim Min‐Cheol , Klapperich Catherine 
Published: November 2010

AbstractA continuous flow polymerase chain reaction (CF‐PCR) device comprises a single fluidic channel that is heated differentially to create spatial temperature variations such that a sample flowing through it experiences the thermal cycling required to induce amplification. This type of device can provide an effective means to detect the presence of a small amount of nucleic acid in very small sample volumes. CF‐PCR is attractive for global health applications due to its less stringent requirements for temperature control than for other designs. For mass production of inexpensive CF‐PCR devices, fabricationviathermoplastic molding will likely be necessary. Here we study the optimization of a PCR assay in a polymeric CF‐PCR device. Three channel designs, with varying residence time ratios for the three PCR steps (denaturation, annealing, and extension), were modeled, built, and tested. A standardized assay was run on the three different chips, and the PCR yields were compared. The temperature gradient profiles of the three designs and the residence times of simulated DNA molecules flowing through each temperature zone were predicted using computational methods. PCR performance predicted by simulation corresponded to experimental results. The effects of DNA template size and cycle time on PCR yield were also studied. The experiments and simulations presented here guided the CF‐PCR chip design and provide a model for predicting the performance of new CF‐PCR designs prior to actual chip manufacture, resulting in faster turn around time for new device and assay design. Taken together, this framework of combined simulation and experimental development has greatly reduced assay development time for CF‐PCR in our lab.
Electrospray ionization‐mass spectrometry conformational analysis of isolated domains of an intrinsically disordered protein
Authors: Testa Lorenzo , Brocca Stefania , Šamalikova Maria , Santambrogio Carlo , Alberghina Lilia , Grandori Rita 
Published: November 2010

AbstractThe highly dynamic and heterogeneous molecular ensembles characterizing intrinsically disordered proteins (IDP) in solution pose major challenges to the conventional methods for structural analysis. Electrospray ionization‐mass spectrometry (ESI‐MS) allows direct detection of distinct conformational components, effectively capturing also partially folded and short‐lived states. We report the description of two complementary fragments (1–186 and 187–284) of the IDP Sic1, a cyclin‐dependent protein kinase inhibitor of yeast Saccharomyces cerevisiae. Structural heterogeneity is noted in both cases, but the two fragments reveal slightly different conformational properties. The results are consistent with previously reported differences between the two protein moieties and corroborate the feasibility of IDP conformational analysis by ESI‐MS.
The molecular structure and solution conformation of an acidic heteropolysaccharide from Auricularia auricula‐judae
Authors: Ma Zhaocheng , Zhang Lina , Nishiyama Yoshiharu , Marais Marie‐France , Mazeau Karim , Vignon Michel 
Published: November 2010

AbstractA water soluble acidic heteropolysaccharide named WAF was isolated from Auricularia auricula‐judae by extracting with 0.9% NaCl solution. By using gas chromatography, gas chromatography‐mass spectrometry, and NMR, its chemical structure was determined to be composed of a backbone of α‐(1→3)‐linked D‐mannopyranose residues with pendant side groups of β‐D‐xylose, β‐D‐glucose, or β‐D‐glucuronic acid at position O6 or O2. Six fractions prepared from WAF with a weight‐average molecular mass (Mw) between 5.9 × 104 and 64.7 × 104 g/mol were characterized with laser light scattering and viscometry in 0.1M NaCl at 25°C. The dependence of intrinsic viscosity ([η]) and radius of gyration (Rg) on Mw for this polysaccharide were found to be [η] = 1.79 × 10−3Mw0.96 cm3 g−1 and Rg = 6.99 × 10−2 Mw0.54 nm. The molar mass per unit contour length (ML) and the persistence length (Lp) were estimated to be 1124 nm−1 and 11 nm, respectively. The WAF exhibited a semirigid character typical of linear polysaccharides. Molecular modeling was then used to predict the ordered and disordered states of WAF; the simulated ML and Lp were however much smaller than the experimental values. Taken altogether, the results suggested that WAF formed a duplex in solution. © 2010 Wiley Periodicals, Inc. Biopolymers 95: 217–227, 2011.
Letter 1: Clinicoradiological score for predicting the risk of strangulated small bowel obstruction (Br J Surg2010; 97; 1119–1125)
Authors: Debnath D , Ogedegbe A J 
Published: November 2010

AbstractThe Editors welcome topical correspondence from readers relating to articles published in the Journal. Responses should be sent electronically via the BJS website (www.bjs.co.uk). All letters will be reviewed and, if approved, appear on the website. A selection of these will be edited and published in the Journal. Letters must be no more than 250 words in length.
Enantiomer Resolution of Intrinsically Chiral C21‐Alkylated N‐Confused Porphyrin Complexes
Authors: Chmielewski Piotr J. , Szterenberg Ludmiła , Siczek Marta 
Published: November 2010

AbstractThe resolution of stereoisomers of C21‐alkylated nickel(II) complexes of N‐confused porphyrin (NCP) was performed by means of chiral‐phase HPLC with an effectiveness of above 90 % molar ratio for each isomer. The reverse signs of the Cotton effects in the circular dichroism (CD) spectra of the separated fractions are indicative of the pair of enantiomers. The application of low‐temperature 2D NMR methods to the separated diastereomers of the system comprising a chiral 2‐(S)‐methylbutyl substituent, in connection with the CD spectra and relative HPLC migration rates, allowed the assignment of the absolute configuration of the chiral C21‐substituted complexes of NCP. The assignment was confirmed by time‐dependent DFT (TDDFT) calculations of CD spectra for the C21‐methylated nickel(II) complex. The system remains chiral after removal of the metal ion from the macrocyclic crevice, despite the fact that this demetalation is connected with a change of the C21 hybridization from pyramidal to trigonal. The retention of chirality was established by means of CD spectra and confirmed by TDDFT calculations for a C21‐methylated NCP free base. Stereoisomers were also separated for three covalently linked bis(NCP) systems with bridges involving one or two C21 carbon atoms. The occurrence of a pair of enantiomers was established for nonsymmetrical dimers comprising only one stereogenic center. In the case of the 21,21′‐(o‐xylene)‐linked dimer, three stereoisomers, that is, a pair of enantiomers and an optically inactive meso‐form, were separated and analyzed by CD and 1H NMR spectroscopy. The stereoisomers of a diastereoselectively formed nonsymmetrical chloroplatinum(II)‐linked dimer, consisting of heterochiral C21‐alkylated NCP nickel(II) subunits, after separation displayed a strong optical activity, which can be ascribed to the rigid helical structure of the complex.
Preparation of Surface Organometallic Catalysts by Gas‐Phase Ligand Stripping and Reactive Landing of Mass‐Selected Ions
Authors: Johnson Grant E. , Laskin Julia 
Published: November 2010

AbstractOrganometallic complexes immobilized on surfaces combine the high selectivity of homogeneous catalysts with the ease of separation of catalyst from products attainable with heterogeneous catalysts. Here we report a novel approach for the highly controlled preparation of surface organometallic catalysts by gas‐phase ligand stripping combined with reactive landing of mass‐selected ions onto self‐assembled monolayer surfaces. Collision‐induced dissociation is used to generate highly reactive undercoordinated metal complexes in the gas‐phase for subsequent surface immobilization. Complexes with an open coordination shell around the metal center are demonstrated to show enhanced activity towards reactive landing in comparison to fully ligated species. In situ TOF‐SIMS analysis indicates that the immobilized complexes exhibit behavior consistent with catalytic activity when exposed to gaseous reagents.
ChemInform Abstract: Square Coordinated MnO2‐Units in BiMn7O12.
Authors: Okamoto Hiroshi , Imamura Naoki , Karppinen Maarit , Yamauchi Hisao , Fjellvaag Helmer 
Published: November 2010

AbstractPolycrystalline samples of BiMn7O12 and LaMn7O12 are characterized by room temperature high‐resolution synchrotron XRD.
ChemInform Abstract: Crystallographic and Spectroscopic Characterization of LnFeTeO6 (Ln: La, Pr, Nd, Sm) Materials.
Authors: Lavat Araceli E. , Mercader Roberto C. , Baran Enrique J. 
Published: November 2010

AbstractThe title compounds are prepared by solid state reactions of stoichiometric mixtures of Ln2O3, Fe2O3, and TeO2 (Pt crucibles, 1000 °C, 2 h).
ChemInform Abstract: [2 + 2] Cycloaddition and Electrophilic Alkynylation Reactions of 4‐Chloro‐1,1,1‐trifluorobut‐3‐yn‐2‐one and Alkyl Vinyl Ethers.
Authors: Koldobskii A. B. , Tsvetkov N. P. , Godovikov I. A. , Kalinin V. N. 
Published: November 2010

AbstractReaction of the title butynone (I) with vinyl ethers results in the parallel formation of [2 + 2] cycloaddition and electrophilic alkynylation products in ratios depending on the nature of the vinyl ether.
ChemInform Abstract: Novel Preparation of Polymer‐Supported Iodobenzene and Its Synthetic Utility as a Recyclable Reagent with m‐Chloroperbenzoic Acid.
Authors: Suzuki Yuhsuku , Togo Hideo 
Published: November 2010

AbstractSome derivatives are developed and the polymer IBP is successfully applied to the α‐tosyloxylation of ketones and to the cyclization of sulfamides.
ChemInform Abstract: TBAF‐Catalyzed Facile Synthesis of Unsymmetrical Diaryl Thioethers via Mild SNAr Reactions.
Authors: Yu Baohua , Zhang Xufeng , Yu Xiaochun , Xu Qing 
Published: November 2010

AbstractSmooth nucleophilic substitution reaction of aryl fluorides with phenylthiotrimethylsilane as nucleophile provides title compounds in excellent yields.
ChemInform Abstract: Novel Naphthalimide—Indomethacin Hybrids as Potential Antitumor Agents: Effects of Linkers on Hypoxic/Oxic Cytotoxicity and Apoptosis‐Inducing Activity.
Authors: Wu Aibin , Xu Yufang , Qian Xuhong 
Published: November 2010

AbstractNovel naphthalimide‐indomethacin hybrids (V) (4 examples) with unique antitumor activity are designed and synthesized.
ChemInform Abstract: Diels—Alder Routes to Angularly Halogenated cis‐Fused Bicyclic Ketones: Readily Accessible Cyclynone Intermediates.
Authors: Lee Jun Hee , Kim Woo Han , Danishefsky Samuel J. 
Published: November 2010

AbstractA simple synthesis is achieved in the presence of MeAlCl2 with unfunctionalized dienes.
ChemInform Abstract: A Facile and Expeditious Approach for the Synthesis of 2‐Azetidinone Derivatives via a Multicomponent Reaction.
Authors: Keri Rangappa S. , Hosamani Kallappa M. 
Published: November 2010

AbstractA simple, efficient, three‐component one‐pot synthesis of the title compounds (IV) (20 examples) using triethylamine as catalyst is developed.
ChemInform Abstract: Efficient Synthesis of New 4‐Arylideneimidazolin‐5‐ones Related to the GFP Chromophore by [2 + 3] Cyclocondensation of Arylideneimines with Imidate Ylides.
Authors: Baldridge Anthony , Kowalik Janusz , Tolbert Laren M. 
Published: November 2010

AbstractThe process offers a convenient access to a variety of arylidene imidazolinones (57 examples).
ChemInform Abstract: Synthesis of Dimers of (4‐Oxo‐4H‐pyran‐2‐yl)acrylic Acid as Tyrosinase Inhibitors.
Authors: Yoon Ik Hwan , Lee Young Hoon , Park Chul Woo , Ji Hyunjun , Lee Yong Sup 
Published: November 2010

AbstractThe title compounds (III) show good inhibition on the melanin production with lower cytotoxicities than the parent compound, pyronyl‐acrylic acid ester.
ChemInform Abstract: Synthesis of Substituted Chromanones: An Organocatalytic Aldol/Oxa‐Michael Reaction.
Authors: Butler Jeffrey D. , Conrad Wayne E. , Kodewyk Michael W. , Fettinger James C. , Tantillo Dean J. , Kurth Mark J. 
Published: November 2010

AbstractCyclopentane‐fused chromanones are diastereoselectively prepared from diketo phenols using pyrrolidine as catalyst.
ChemInform Abstract: Synthesis and Biological Significance of Pyrano Pyrazoles from 8,9‐Dimethyl‐3‐acetoacetyl Pyrano[3,2‐c][1]benzopyran‐2,5‐dione (III).
Authors: Siddiqui Zeba N. , Asad Mohammad 
Published: November 2010

AbstractCompounds (III) and (Vb) show moderate antibacterial activities against E.coli and S.dysenteriae.
ChemInform Abstract: Pd2(dba)3‐Promoted Synthesis of 3‐N‐Substituted 4‐Aryl‐1,2,3,6‐tetrahydropyridine.
Authors: Chang Meng‐Yang , Lin Chung‐Han , Chen Yeh‐Long , Hsu Ru‐Ting , Chang Ching‐Yao 
Published: November 2010

AbstractAllylic bromination of tetrahydropyridines affords suitable precursors for a Pd‐catalyzed cross‐coupling with various N‐nucleophiles.
ChemInform Abstract: Diastereoselective Approach to Novel Octahydroisoquinolones and an Extension to Its One‐Pot Synthesis.
Authors: Singh Pardeep , Bhargava Gaurav , Kumar Vipan , Mahajan Mohinder P. 
Published: November 2010

AbstractA two‐step sequence involving cleavage of β‐lactams with sodium methoxide and subsequent cyclization of the resulting amino esters affords novel diastereomerically pure title compounds (IV).
ChemInform Abstract: Multicomponent Tandem Synthesis of Oxospirobicyclic Butenolidobarbiturates.
Authors: Teimouri Mohammad Bagher , Abbasi Tayyebeh , Khavasi Hamid Reza 
Published: November 2010

AbstractThe sequence of this new one‐pot, multicomponent reaction involves a 1,4‐Michael addition, aldol‐like reaction, lactonization, 1,4‐addition‐elimination and an acid‐base reaction and leads to biologically relevant oxospirocyclic compounds (IV), containing two different pharmacophoric subunits.
ChemInform Abstract: Synthesis and Biological Activity of 6‐Alkyl/Chloro‐3‐{4‐(6‐alkyl/chloro‐2H‐benzo[e] [1,3]oxazin‐3(4H)‐yl)phenyl}‐3,4‐dihydro‐2H‐benzo[e][1,3]oxazines.
Authors: Manikannan Ramaiyan , Muthusubramanian Shanmugam 
Published: November 2010

AbstractAn efficient synthesis of symmetrical title compounds (8 examples) includes 2 steps and microwave irradiation.
ChemInform Abstract: Novel Method for the Synthesis of s‐Triazolo[3,4‐b][1,3,4]thiadiazines.
Authors: El‐Sherief Hassan A. H. , Hozien Zeniab A. , El‐Mahdy Ahmed F. M. , Sarhan Abdelwareth A. O. 
Published: November 2010

AbstractThe acid‐promoted condensation of the triazole (I) with a variety of ketones results in formation of triazolothiadiazines (III) in good yields.
ChemInform Abstract: Synthesis of Azepino[3,4b]indoles via the Plancher Rearrangement.
Authors: Eisenbeis Shane A. , Phillips James R. , Rescek Diane , Oyola‐Cintron Yatsandra 
Published: November 2010

AbstractAryl hydrazines react with formyl‐substituted saturated N‐heterocycles to form fused indoles after reductive work‐up.
ChemInform Abstract: Chiral N‐Phosphoryl Imines: Design, Synthesis and Direct Asymmetric Addition Reactions with Diketones and Diesters.
Authors: Sun Hao , Rajale Trideep , Pan Yi , Li Guigen 
Published: November 2010

AbstractImines (I) are investigated as electrophiles in the asymmetric addition to different 1,3‐dicarbonyl compounds.
ChemInform Abstract: Diastereoselective Addition of Di‐(trimethylsilyl)phosphite to Chiral N‐(R)‐α‐Methylbenzyl and N‐(1‐Methoxycarbonyl‐iso‐pentyl) Schiff Bases of Various Aldehydes.
Authors: Lewkowski Jaroslaw , Karpowicz Rafal 
Published: November 2010

AbstractThe addition of bis(trimethylsilyl)phosphite, generated in situ by the reaction of diethyl phosphite with bromotrimethylsilane, to two groups of imines is performed, the chiral Schiff bases (I) and imines (III).
ChemInform Abstract: Surugapyrone A from Streptomyces coelicoflavus Strain USF‐6280 as a New DPPH Radical‐Scavenger
Authors: Sugiyama Ysumasa , Oya Akiko , Kudo Takuji , Hirota Akira 
Published: November 2010

Abstractisolation, structure elucidation and biosynthesis of surugapyrone A (I)
ChemInform Abstract: A Stereoselective Aldol Approach for the Total Synthesis of Herbarumin I (VIIIb) and Stagonolide A (IX).
Authors: Srihari P. , Rao G. Maheswara , Rao R. Srinivasa , Yadav J. S. 
Published: November 2010

AbstractThe synthesis includes the Crimmins protocol for the non‐Evans anti‐aldol reaction of aldehyde (II), a stereoselective Grignard reaction, and Grubbs′ ring‐closing metathesis as the key steps.
StarPU: a unified platform for task scheduling on heterogeneous multicore architectures
Authors: Augonnet Cédric , Thibault Samuel , Namyst Raymond , Wacrenier Pierre‐André 
Published: November 2010

AbstractIn the field of HPC, the current hardware trend is to design multiprocessor architectures featuring heterogeneous technologies such as specialized coprocessors (e.g. Cell/BE) or data‐parallel accelerators (e.g. GPUs). Approaching the theoretical performance of these architectures is a complex issue. Indeed, substantial efforts have already been devoted to efficiently offload parts of the computations. However, designing an execution model that unifies all computing units and associated embedded memory remains a main challenge. We therefore designed StarPU, an original runtime system providing a high‐level, unified execution model tightly coupled with an expressive data management library. The main goal of StarPU is to provide numerical kernel designers with a convenient way to generate parallel tasks over heterogeneous hardware on the one hand, and easily develop and tune powerful scheduling algorithms on the other hand. We have developed several strategies that can be selected seamlessly at run‐time, and we have analyzed their efficiency on several algorithms running simultaneously over multiple cores and a GPU. In addition to substantial improvements regarding execution times, we have obtained consistentsuperlinearparallelism by actuallyexploitingthe heterogeneous nature of the machine. We eventually show that our dynamic approach competes with the highly optimized MAGMA library and overcomes the limitations of the corresponding static scheduling in a portable way. Copyright © 2010 John Wiley & Sons, Ltd.
Pure exchange markets for resource sharing in federated clouds
Authors: Gomes Eduardo R. , Vo Quoc Bao , Kowalczyk Ryszard 
Published: November 2010

SUMMARYCloud Computing is the latest paradigm proposed toward fulfilling the vision of computing being delivered as an utility such as phone, electricity, gas and water services. It enables users to have access to computing infrastructure, platform and software as services over the Internet. The services can be accessed on demand and from anywhere in the world in a quick and flexible manner, and charged for based on their usage, making the rapid and often unpredictable expansion demanded by nowadays' business environment affordable also for small spin‐off and start‐up companies. In order to be competitive, however, Cloud providers need to be able to adapt to the dynamic loads from users, not only optimizing the local usage and costs but also engaging into agreements with other Clouds so as to complement local capacity. The infrastructure in which competing Clouds are able to cooperate to maximize their benefits is called a Federated Cloud. Just as Clouds enable users to cope with unexpected demand loads, a Federated Cloud will enable individual Clouds to cope with unforeseen variations of demand. The definition of the mechanism to ensure mutual benefits for the individual Clouds composing the federation, however, is one of its main challenges. This paper proposes and investigates the application of market‐oriented mechanisms based on the General Equilibrium Theory of Microeconomics to coordinate the sharing of resources between the Clouds in the Federated Cloud. Copyright © 2010 John Wiley & Sons, Ltd.
A decentralized coordination method for optimal load redistribution in heterogeneous service grids
Authors: Chen Ying‐Ying , Wu Tie‐Jun 
Published: November 2010

AbstractThe optimal load redistribution problem is solved in this paper for heterogeneous non‐dedicated service grids in a decentralized way. A coordination policy is proposed to make networked servers reach their optimal generic task acceptance rates in order to minimize the average service time of all the generic tasks in a grid. Autonomous servers networked in the grid only need to coordinate with their neighbors iteratively, and their optimal generic acceptance rates are reached by task migration among them. The design scheme of the policy is introduced, and the convergence properties and the implementation aspects of the coordination system are discussed in detail in this paper. A set of computer simulations have been conducted, validating the effectiveness of the proposed approach. Copyright © 2010 John Wiley & Sons, Ltd.