bluuebunny/crossref_metadata_2025_split huggingface dataset:
Antibacterial, SOD mimic and nuclease activities of copper(II) complexes containing ofloxacin and neutral bidentate ligands
Authors: Patel Mohan N. , Parmar Pradhuman A. , Gandhi Deepen S. 
Published: June 2010

AbstractDrug‐based mixed‐ligand copper(II) complexes of type [Cu(OFL)(An)Cl]·5H2O (OFL = ofloxacin, A1 = pyridine‐2‐carbaldehyde, A2 = 2,2′‐bipyridylamine, A3 = thiophene‐2‐carbaldehyde, A4 = 2,9‐dimethyl‐1,10‐phenanthroline, A5 = 2,9‐dimethyl‐4,7‐diphenyl‐1,10‐phenanthroline, A6 = 4,5‐diazafluoren‐9‐one, A7 = 1,10‐phenanthroline‐5,6‐dione and A8 = 5‐nitro‐1,10‐phenanthroline) were synthesized and characterized. Spectral investigations of complexes revealed square pyramidal geometry. Viscosity measurement and absorption titration were employed to determine the mode of binding of complexes with DNA. DNA cleavage study showed better cleaving ability of the complexes compared with metal salt and standard drug by conversion of a supercoiled form of pUC19 DNA to linear via circular. From the SOD mimic study, concentration of complexes ranging from 0.415 to 1.305 µM is enough to inhibit the reduction rate of NBT by 50% (IC50) in the NADH‐PMS system. Antibacterial activity was assayed against selective Gram‐negative and Gram‐positive microorganisms using the doubling dilution technique. Copyright © 2010 John Wiley & Sons, Ltd.
Cumulative conformance count chart with variable sampling intervals and control limits
Authors: Chen Yan‐Kwang , Chen Chien‐Yue , Chiou Kuo‐Ching 
Published: June 2010

AbstractThe cumulative conformance count (CCC) chart has been used for monitoring processes with very low fraction of nonconforming items. Typically, the items produced from the process were examined using 100% inspection for generating the CCC chart. However, this would be costly when taking the inspection cost and time into consideration and thus limit its application. Instead of inspecting the items one by one, this study takes sample from them, and regards the time between two successive samples as the sampling interval. In order to increase the sensitivity of the CCC chart to process change, the sampling interval and control limits are allowed to vary in this study. The average time to signal process change of the modified CCC chart (called the variable sampling interval and control limit (VSI/VCL) CCC chart) is derived by the Markov chain approach and taken as the performance measure to evaluate its statistical efficiency. With some minor changes, this chart can be reduced to the VSI CCC chart, the VCL CCC chart, and the standard CCC chart. In addition, comparisons among them are made and discussed. Copyright © 2010 John Wiley & Sons, Ltd.
Dose‐dependent pharmacokinetics of SP‐8203 in rats
Authors: Lee Joo H. , Choi Young H. , Suh Jung H. , Kang Hee E. , Lee Tae H. , Cho Il H. , Lee Myung G. 
Published: June 2010

AbstractThe pharmacokinetics of SP‐8203, a potential protective agent for the treatment of cerebral infarction, were evaluated after its intravenous (10, 20 and 30 mg/kg) and oral (10, 20, 30 and 100 mg/kg) administration in rats. After the intravenous administration of SP‐8203, the AUCs of SP‐8203 were dose‐dependent; the dose‐normalized AUCs were significantly greater with increasing doses. After the oral administration of SP‐8203, plasma concentrations of SP‐8203 were much lower than those after intravenous administration. This could be due to considerable hepatic and intestinal metabolism and the high percent of the dose recovered from the gastrointestinal tract (including its contents and feces) at 24 h as unchanged drug. Copyright © 2010 John Wiley & Sons, Ltd.
Is the coral‐algae symbiosis really ‘mutually beneficial’ for the partners?
Authors: Wooldridge Scott A. 
Published: June 2010

AbstractThe consideration of ‘mutual benefits’ and partner cooperation have long been the accepted standpoint from which to draw inference about the onset, maintenance and breakdown of the coral‐algae endosymbiosis. In this paper, I review recent research into the climate‐induced breakdown of this important symbiosis (namely ‘coral bleaching’) that challenges the validity of this long‐standing belief. Indeed, I introduce a more parsimonious explanation, in which the coral host exerts a ‘controlled parasitism’ over its algal symbionts that is akin to an enforced domestication arrangement. Far from being pathogenic, a range of well‐established cellular processes are reviewed that support the role of the coral host as an active ‘farmer’ of the energy‐rich photoassimilates from its captive symbionts. Importantly, this new paradigm reposes the deleterious bleaching response in terms of an envelope of environmental conditions in which the exploitative and captive measures of the coral host are severely restricted. The ramification of this new paradigm for developing management strategies that may assist the evolution of bleaching resistance in corals is discussed.
Technical refolding of proteins: Do we have freedom to operate?
Authors: Eiberle Maria K. , Jungbauer Alois 
Published: June 2010

AbstractExpression as inclusion bodies in Escherichia coli is a widely used method for the large‐scale production of therapeutic proteins that do not require post‐translational modifications. High expression yields and simple recovery steps of inclusion bodies from the host cells are attractive features industrially. However, the value of an inclusion body‐based process is dominated by the solubilization and refolding technologies. Scale‐invariant technologies that are economical and applicable for a wide range of proteins are requested by industry. The main challenge is to convert the denatured protein into its native conformation at high yields. Refolding competes with misfolding and aggregation. Thus, the yield of native monomer depends strongly on the initial protein concentrations in the refolding solution. Reasonable yields are attained at low concentrations (≤0.1 mg/mL). However, large buffer tanks and time‐consuming concentration steps are required. We attempt to answer the question of the extent to which refolding of proteins is protected by patents. Low‐molecular mass additives have been developed to improve refolding yields through the stabilization of the protein in solution and shielding hydrophobic patches. Progress has been made in the field of high‐pressure renaturation and on‐column refolding. Mixing times of the denatured protein in the refolding buffer have been reduced using newly developed devices and the introduction of specific mixers. Concepts of continuous refolding have been introduced to reduce tank sizes and increase yields. Some of the patents covering refolding of proteins will soon expire or have already expired. This gives more freedom to operate.
Electric pulses to prepare feeder cells for sustaining and culturing of undifferentiated embryonic stem cells
Authors: Browning Lauren M. , Xu Xiao‐Hong Nancy 
Published: June 2010

AbstractCurrent challenges in embryonic‐stem cell (ESC) research include the inability of sustaining and culturing of undifferentiated ESCs over time. Growth‐arrested feeder cells are essential to the culture and sustaining of undifferentiated ESCs, and they are currently prepared using gamma‐radiation and chemical inactivation. Both techniques have severe limitations. In this study, we developed a new, simple and effective technique (pulsed electric fields, PEFs) to produce viable growth‐arrested cells (RTS34st) and used them as high‐quality feeder cells to culture and sustain undifferentiated zebrafish ESCs over time. The cells were exposed to 25 sequential 10‐ns electric pulses (10nsEPs) of 25, 40 and 150 kV/cm with 1‐s pulse interval, or 2 sequential 50‐μs electric pulses (50μsEPs) of 2.83, 1.78 and 0.78 kV/cm with 5‐s pulse interval, respectively. We found that the cellular effects of PEFs depended directly upon the duration, number and electric field strength of the pulses, showing the feasibility of tuning them to produce various types of growth‐arrested cells for culturing undifferentiated ESCs. Both 10nsEPs of 40 kV/cm produced by a 10nsEP generator and 50μsEPs of 1.78 kV/cm provided by inexpensive and widely available conventional electroporators, generated high‐quality growth‐arrested feeder cells for proliferation of undifferentiated ESCs over time. PEFs can therefore be used to replace radiation and chemical inactivation methods for preparation of growth‐arrested feeder cells for advancing ESC research.
Development and validation of a rapid and sensitive LC‐ESI‐MS/MS method for ondansetron quantification in human plasma and its application in comparative bioavailability study
Authors: Moreira Roberto F. , Salvadori Myriam C. , Azevedo Cristina P. , Oliveira‐Silva Diogo , Borges Diego C. , Moreno Ronílson A. , Sverdloff Carlos E. , Borges Ney C. 
Published: June 2010

AbstractThe validation of a high throughput and specific method using a high‐performance liquid chromatography coupled to electrospray (ES+) ionization tandem triple quadrupole mass spectrometric (LC‐ESI‐MS/MS) method for ondansetron quantification in human plasma is described. Human plasma samples were extracted by liquid–liquid extraction (LLE) using methyl tert‐butyl ether and analyzed by LC‐ESI‐MS/MS. The limit of quantification was 0.2 ng/mL and the method was linear in the range 0.2–60 ng/mL. The intra‐assay precisions ranged from 1.6 to 7.7%, while inter‐assay precisions ranged from 2.1 to 5.1%. The intra‐assay accuracies ranged from 97.5 to 108.2%, and the inter‐assay accuracies ranged from 97.3 to 107.0%. The analytical method was applied to evaluate the relative bioavailability of two pharmaceutical formulations containing 8 mg of ondansetron each in 25 healthy volunteers using a randomized, two‐period crossover design. The geometric mean and respective 90% confidence interval (CI) of ondansetron test/reference percent ratios were 90.15% (81.74–99.44%) forCmaxand 93.11% (83.01–104.43%) for AUC0–t. Based on the 90% confidence interval of the individual ratios (test formulation/reference formulation) forCmaxand AUC0‐inf, it was concluded that the test formulation is bioequivalent to the reference one with respect to the rate and extent of absorption of ondansetron. Copyright © 2010 John Wiley & Sons, Ltd.
Profiling of Piper betle Linn. cultivars by direct analysis in real time mass spectrometric technique
Authors: Bajpai Vikas , Sharma Deepty , Kumar Brijesh , Madhusudanan K. P. 
Published: June 2010

AbstractPiper betle Linn. is a traditional plant associated with the Asian and southeast Asian cultures. Its use is also recorded in folk medicines in these regions. Several of its medicinal properties have recently been proven. Phytochemical analysis showed the presence of mainly terpenes and phenols in betel leaves. These constituents vary in the different cultivars of Piper betle. In this paper we have attempted to profile eight locally available betel cultivars using the recently developed mass spectral ionization technique of direct analysis in real time (DART). Principal component analysis has also been employed to analyze the DART MS data of these betel cultivars. The results show that the cultivars of Piper betle could be differentiated using DART MS data. Copyright © 2010 John Wiley & Sons, Ltd.
Development and validation of an LC‐ESI‐MS/MS method for simultaneous quantitation of olmesartan and pioglitazone in rat plasma and its pharmacokinetic application
Authors: Sengupta Pinaki , Sarkar Amlan Kanti , Bhaumik Uttam , Chatterjee Bappaditya , Roy Bikash , Chakraborty Uday Shankar , Pal Tapan Kumar 
Published: June 2010

AbstractA simple, high‐throughput and specific high‐performance liquid chromatography tandem mass spectrometry method has been developed and validated according to the FDA guidelines for simultaneous quantification of olmesartan and pioglitazone in rat plasma. The bioanalytical method consists of liquid–liquid extraction and quantitation by triple quadrupole mass spectrometry using electrospray ionization technique, operating in multiple reaction monitoring and positive ion modes. The compounds were eluted isocratically on a C18column with a mobile phase consisting of a mixture of methanol and water (containing 0.5% formic acid) in a ratio of 9:1. The response to olmesartan and pioglitazone was linear over the range 0.01–10 µg/mL. The validation results demonstrated that the method had satisfactory precision and accuracy across the calibration range. Intra‐ and inter‐day precisions ranged from 0.66 to 3.32 and from 0.94 to 2.93% (%CV), respectively. The accuracy determined at three quality control levels was within 91.27–107.28%. There was no evidence of instability of the analytes in rat plasma following the stability studies. The method proved highly reproducible and sensitive and was successfully applied in a pharmacokinetic study after single dose oral administration of olmesartan and pioglitazone to the rat. Copyright © 2010 John Wiley & Sons, Ltd.
Application of liquid chromatography–tandem mass spectrometry method for the simultaneous quantitative analysis of propentofylline and its chiral metabolite M1 in rats
Authors: Walczak Maria , Kozaczek Ewelina , Szymura‐Oleksiak Joanna , Pękala Elżbieta 
Published: June 2010

AbstractA sensitive and selective liquid chromatographic–electrospray ionization mass spectrometric method for the simultaneous determination of propentofylline and enantiomers of its active metabolite M1 in rat serum, cortex and hippocampus was developed and validated according to GLP procedures. Sample preparations were carried out by liquid–liquid extraction using diethyl ether after the addition of the internal standard (pentoxifylline). The dried residue was reconstituted in mobile phase and injected onto a Chiralpak AD column (10 µm, 250 × 4.6 mm i.d.). The limit of quantification for propentofylline in serum, cortex and hippocampus was set at 0.25 ng/mL and for enantiomers of its metabolite M1 at 1.25 ng/mL. The established LC/ESI‐MS/MS method has been successfully applied to an initial pharmacokinetic study of propentofylline and also to assessment of distribution of parent drug and enantiomers of its pharmacologically active metabolite M1 to cortex and hippocampus after intravenous administration of propentofylline to rats at a dose of 5 mg/kg. Copyright © 2010 John Wiley & Sons, Ltd.
Ionic liquid aqueous solvent‐based microwave‐assisted hydrolysis for the extraction and HPLC determination of myricetin and quercetin from Myrica rubra leaves
Authors: Du Fu‐You , Xiao Xiao‐Hua , Li Gong‐Ke 
Published: June 2010

AbstractAn ionic liquid aqueous solvent‐based microwave‐assisted hydrolysis (ILAS‐MAH) approach was proposed for the rapid extraction and accurate determination of myricetin and quercetin from Myrica rubra (M. rubra) leaves for the first time. The effects of the ionic liquid class and concentration, liquid–solid ratio, hydrolysis temperature and time were investigated to obtain the optimal ILAS‐MAH conditions. The optimized conditions were 2.0 mol/L [bmin][HSO4] or 2.5 mol/L acidified [bmin]Br solution, liquid–solid ratio 30:1 (mL : g), hydrolysis temperature 70°C and hydrolysis time 10 min. Under these conditions, the recoveries of myricetin and quercetin were in the range of 86.3–107.3% with relative standard deviation lower than 5.8%. Compared with conventional heating hydrolysis and regular MAH, the proposed approach reduced hydrolysis time and improved yields. The mechanism of ILAS‐MAH was also investigated. Copyright © 2010 John Wiley & Sons, Ltd.
ChemInform Abstract: A Three‐Dimensional Neutral Framework of a Novel Decavanadium Cluster Bridged by an AsO4 Tetrahedron: [AsVIV8VV2O26 (μ‐H2O)]×8H2O.
Authors: Bu Wei‐Ming , Ye Ling , Yang Guo‐Yu , Shao Mei‐Cheng , Fan Yu‐Guo , Xu Ji‐Qing 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Aluminum Fluoride as a Storage Matrix for Atomic Hydrogen.
Authors: Scholz Gudrun , Stoesser Reinhard , Momand Jan Alam , Zehl Andrea , Klein Jeannette 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Straightforward Conversion Route to Nanocrystalline Monothiooxides of Rare Earths Through a High‐Temperature Colloid Technique.
Authors: Li Yadong , Huang Yu , Bai Tao , Li Longquan 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis and Investigations of MIP1‐xAsxUO6×nH2 O (MI: H, Li, Na, K, Rb, Cs) Solid Solutions.
Authors: Chernorukov N. G. , Karyakin N. V. , Suleimanov E. V. , Ermonin S. A. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Intramolecular Homolytic Displacements. Part 30. Functional Decarbonylative Transformations of Aldehydes via Homolytically Induced Decomposition of Unsaturated Peroxyacetals.
Authors: Degueil‐Castaing Marie , Moutet Laurent , Maillard Bernard 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Isomerization Reaction of Olefin Using RuClH(CO)(PPh3)3.
Authors: Wakamatsu Hideaki , Nishida Mayumi , Adachi Naoko , Mori Miwako 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Sulfuration of Organoborates, an Underexploited Method.
Authors: Kerverdo Sebastien , Gingras Marc 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Remarkable Rate Enhancement of Palladium‐Catalyzed Allylic Alkylation in Water Using a Colloidal Dispersion System.
Authors: Kobayashi Shu , Lam William W.‐L. , Manabe Kei 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Carbenoid Insertion into Alkenylzirconocenes — A Convergent Synthesis of Functionalized Allylmetallics.
Authors: Kasatkin Alexander N. , Whitby Richard J. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: New Synthetic Protocol with Trifluoro‐1‐propynylamines. An Efficient Access to α‐(Trifluoromethyl)‐δ‐keto Amides via Hetero Diels—Alder Reaction.
Authors: Mantani Toshiya , Konno Tsutomu , Ishihara Takashi , Yamanaka Hiroki 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Modification of the Tiemann Rearrangement: One‐Pot Synthesis of N,N‐Disubstituted Cyanamides from Amidoximes.
Authors: Bakunov Stanislav A. , Rukavishnikov Alexey V. , Tkachev Alexey V. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A New Synthesis of Racemic Rosaprostol.
Authors: Mikolajczyk Marian , Mikina Maciej , Jankowiak Aleksandra , Mphahlele Malose J. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Base‐Catalyzed Domino‐Isomerization—Hydroamination Reaction — A New Synthetic Route to Amphetamines.
Authors: Hartung Christian G. , Breindl Claudia , Tillack Annegret , Beller Matthias 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Catalytic Hydrogen Transfer over Magnesia. Part 13. Liquid Phase Reduction of Substituted 1‐Phenyl‐1‐alkanones by 2‐Octanol.
Authors: Glinski M. 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Dramatic Change of Regioselectivity in the Addition of Silylated Organolithium Reagents with Oxazolidines Induced by the Presence of a Titanium Salt.
Authors: Agami Claude , Comesse Sebastien , Kadouri‐Puchot Catherine 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Simple Synthesis of Highly Functionalized Ketenimines.
Authors: Yavari Issa , Nourmohammadian Farahnaz 
Published: June 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.