bluuebunny/crossref_metadata_2025_split huggingface dataset:
Thyroid follicular lesion of undetermined significance: Evaluation of the risk of malignancy using the two‐tier sub‐classification
Authors: Horne Matthew J. , Chhieng David C. , Theoharis Constantine , Schofield Kevin , Kowalski Diane , Prasad Manju L. , Hammers Lynwood , Udelsman Robert , Adeniran Adebowale J. 
Published: September 2011

AbstractThe Bethesda 2007 Thyroid Cytology Classification defines follicular lesion of undetermined significance as a heterogeneous category of cases that are not convincingly benign nor sufficiently atypical for a diagnosis of follicular neoplasm or suspicious for malignancy. In our institution, we refer to these cases as indeterminate, and they are further sub‐classified into two: (1) low cellularity with predominant microfollicular architecture and absence of colloid (INa) and (2) nuclear features not characteristic of benign lesions (nuclear atypia) (INb). We reviewed these indeterminate cases to document the follow‐up trend using this two‐tier classification. A search of the cytology records was performed for the period between January 2008 and June 2009. All thyroid fine‐needle aspiration (FNA) cases were reviewed and the ones diagnosed as indeterminate were identified. Correlating follow‐up FNA and/or surgical pathology reports were reviewed. The percentage of cases showing a malignancy was calculated. One hundred and seventy‐one indeterminate cases were identified, representing 2.8% of the 6,205 thyroid FNA cases examined during the time under review (107 INa, 64 INb). Records of follow‐up procedures were available in 106 (61%) cases. Malignancy was identified in 27% of all indeterminate cases. This was disproportionately more in the INb (56%) compared to the INa (7%) cases. A diagnosis of “INa” does not carry the same implication as that of “INb”. The INb category needs a more aggressive follow‐up than the INa category and may justify an immediate referral for lobectomy. Despite the vague morphologic criteria for this diagnostic category, the indeterminate rate remains relatively low and falls within the NCI recommendation (<7%). Diagn. Cytopathol. 2011;. © 2011 Wiley‐Liss, Inc.
Fluorescent in situ hybridization diagnosis of extramedullary nodal blast crisis
Authors: Jin Guan‐Nan , Zou Ping , Chen Wan‐Xin , Ding Ze‐Yang , Zhou Hao 
Published: September 2011

AbstractThe t(9;22)(q34;q11) translocation between bcr and abl genes plays a pivotal role in the pathogenesis and diagnosis of chronic myelogenous leukemia (CML). Fluorescence in situ hybridization (FISH) using specific DNA probes provides a useful and accurate way for the detection of bcr/abl fusion gene in single cell. Here, we report an unusual case of a patient with no prior hematologic disease who initially manifested lymphadenopathy. The lymph node findings were suspicious for T‐lineage lymphoblastic lymphoma, however, his blood and bone marrow at that time were in chronic phase of CML. This presented difficulty for accurate discrimination between CML blast crisis (BC) and non‐Hodgkin's lymphomas (NHLs). To discern where the extramedullary nodal malignancy originated from, we cytologically analyzed lymph node biopsies and bone marrow with FISH to detect bcr/abl fusion signals. Together with the morphology, immunohistochemistry, cytogenetics as well as molecular analysis, the patient was diagnosed as extramedullary T‐lymphoid BC of Ph+ CML. In conclusion, this case is unusual at three levels: first, extramedullary nodal BC as a presenting manifestation of CML is rare and the blasts are of precursor T lymphoblastic lineage, rather than the more common B‐cell lineage; second, this case suggests that extramedullary lymphoid nodal BC of CML can exist independently without the bone marrow developing into BC; and third, FISH analysis on the single neoplastic cell is an accurate way to confirm that the neoplasm is either extramedullary localized blasts of CML or genetically distinct neoplasm. Diagn. Cytopathol. 2013. © 2011 Wiley Periodicals, Inc.
A large scale screen for neural stem cell markers in Xenopus retina
Authors: Parain Karine , Mazurier Nicolas , Bronchain Odile , Borday Caroline , Cabochette Pauline , Chesneau Albert , Colozza Gabriele , Yakoubi Warif El , Hamdache Johanna , Locker Morgane , Gilchrist Michael J. , Pollet Nicolas , Perron Muriel 
Published: September 2011

AbstractNeural stem cell research suffers from a lack of molecular markers to specifically assess stem or progenitor cell properties. The organization of the Xenopus ciliary marginal zone (CMZ) in the retina allows the spatial distinction of these two cell types: stem cells are confined to the most peripheral region, while progenitors are more central. Despite this clear advantage, very few genes specifically expressed in retinal stem cells have been discovered so far in this model. To gain insight into the molecular signature of these cells, we performed a large‐scale expression screen in the Xenopus CMZ, establishing it as a model system for stem cell gene profiling. Eighteen genes expressed specifically in the CMZ stem cell compartment were retrieved and are discussed here. These encode various types of proteins, including factors associated with proliferation, mitotic spindle organization, DNA/RNA processing, and cell adhesion. In addition, the publication of this work in a special issue on Xenopus prompted us to give a more general illustration of the value of large‐scale screens in this model species. Thus, beyond neural stem cell specific genes, we give a broader highlight of our screen outcome, describing in particular other retinal cell markers that we found. Finally, we present how these can all be easily retrieved through a novel module we developed in the web‐based annotation tool XenMARK, and illustrate the potential of this powerful searchable database in the context of the retina. © 2011 Wiley Periodicals, Inc. Develop Neurobiol 72: 491–506, 2012
Hematopoietic prostaglandin D synthase (H‐Pgds) is expressed in the early embryonic gonad and participates to the initial nuclear translocation of the SOX9 protein
Authors: Moniot Brigitte , Farhat Andalib , Aritake Kosuke , Declosmenil Faustine , Nef Serge , Eguchi Naomi , Urade Yoshihiro , Poulat Francis , Boizet‐Bonhoure Brigitte 
Published: September 2011

AbstractIn mammals, the Prostaglandin D2 (PGD2) signaling pathway is involved in male gonadal development, regulating Sox9 gene expression and SOX9 protein subcellular localization through lipocalin prostaglandin D synthase (L‐Pgds) activity. Nevertheless, because L‐Pgds is downstream of Sox9, its expression cannot explain the initial nuclear translocation of the SOX9 protein. Here, we show that another source of PGD2, hematopoietic‐Pgds (H‐Pgds) enzyme is expressed in somatic and germ cells of the embryonic gonad of both sexes, as early as embryonic day (E) 10.5, before the onset of L‐Pgds expression. Inhibition of H‐Pgds activity by the specific HQL‐79 inhibitor leads to impaired nuclear translocation of SOX9 protein in E11.5 Sertoli cells. Furthermore, analysis of H‐Pgds−/− male embryonic gonads confirms abnormal subcellular localization of SOX9 protein at the E11.5 early stage of mouse testicular differentiation suggesting a role for H‐Pgds‐produced PGD2 in the initial nuclear translocation of SOX9. Developmental Dynamics 240:2335–2343, 2011. © 2011 Wiley‐Liss, Inc.
DNApol‐ϵ gene is indispensable for the survival and growth of Drosophila melanogaster
Authors: Verma Akanksha , Sengupta Sonali , Lakhotia Subhash C. 
Published: September 2011

AbstractBased on deletion and complementation mapping and DNA sequencing, a new recessive fully penetrant mutation (DNApol‐ϵpl10R), causing prolonged larval life and larval/early pupal lethality, is identified as the first mutant allele of the DNApol‐ϵ (CG6768) gene of Drosophila melanogaster. A same‐sense base pair substitution in exon 1 of the DNApol‐ϵ gene is associated with retention of the first intron and significant reduction in DNApol‐ϵ transcripts in DNApol‐ϵpl10R homozygotes. Homozygous mutant larvae show small imaginal discs with fewer cells and reduced polyteny in salivary glands, presumably because of the compromised DNA polymerase function following exhaustion of the maternal contribution. Extremely small and rare DNApol‐ϵpl10R homozygous somatic clones in DNApol‐ϵpl10R/+ imaginal discs confirm their poor mitotic activity. The DNApol‐ϵpl10R homozygotes, like those expressing DNApol‐ϵ‐RNAi transgene, show high sensitivity to DNA damaging agents. The first mutant allele of the DNApol‐ϵ gene will facilitate functional characterization of this enzyme in the genetically tractable Drosophila model. genesis 50:86–101, 2012. © 2011 Wiley Periodicals, Inc.
Effects of reducing the frequency and duration criteria for binge eating on lifetime prevalence of bulimia nervosa and binge eating disorder: Implications for DSM‐5
Authors: Trace Sara E. , Thornton Laura M. , Root Tammy L. , Mazzeo Suzanne E. , Lichtenstein Paul , Pedersen Nancy L. , Bulik Cynthia M. 
Published: September 2011

AbstractObjective:We assessed the impact of reducing the binge eating frequency and duration thresholds on the diagnostic criteria for bulimia nervosa (BN) and binge eating disorder (BED).Method:We estimated the lifetime population prevalence of BN and BED in 13,295 female twins from the Swedish Twin study of Adults: Genes and Environment employing a range of frequency and duration thresholds. External validation (risk to cotwin) was used to investigate empirical evidence for an optimal binge eating frequency threshold.Results:The lifetime prevalence estimates of BN and BED increased linearly as the frequency criterion decreased. As the required duration increased, the prevalence of BED decreased slightly. Discontinuity in cotwin risk was observed in BN between at least four times per month and at least five times per month. This model could not be fit for BED.Discussion:The proposed changes to the DSM‐5 binge eating frequency and duration criteria would allow for better detection of binge eating pathology without resulting in a markedly higher lifetime prevalence of BN or BED. © 2011 by Wiley Periodicals, Inc. (Int J Eat Disord 2012)
Bulimia nervosa presenting as rectal purging and rectal prolapse: Case report and literature review
Authors: Guerdjikova Anna I. , O'Melia Anne , Riffe Kris , Palumbo Todd , McElroy Susan L. 
Published: September 2011

AbstractRectal prolapse, but not rectal purging (excessive finger evacuation to induce defecation), has been formally associated with eating disorders in the medical literature. We describe a young woman with bulimia nervosa and irritable bowel syndrome who used rectal purging as a method of counteracting the effects of her binge eating and who underwent two corrective surgeries for rectal prolapse in a 15‐month interval. Further research into the relationship between eating disorders, rectal purging, and gastrointestinal dysfunction is called for. © 2011 by Wiley Periodicals, Inc. (Int J Eat Disord 2012)
Comprehensive numerical analysis of fault‐structure systems – Computation of the large‐scale seismic structural response to a given earthquake scenario –
Authors: Ichimura T. , Hori M. , Quinay P. E. , Wijerathne M. L. L. , Suzuki T. , Noguchi S. 
Published: September 2011

SUMMARYThe seismic structural response is affected by temporal and spatial variations in strong ground motion. It can be evaluated through the fault‐structure system: the fault mechanism, wave propagation through the crust, amplification near the surface, and soil‐structure interaction. To analyze this system at high resolution and accuracy, we previously proposed a new multiscale analysis method and numerically verified its validity. However, the problem of the extremely large computation cost of constructing a three‐dimensional numerical model and solving the discretized governing equations still remains. Here, we introduce a new method to resolve these difficulties. By combining this new method with our multiscale analysis, we developed a tool for fault‐structure system analysis. The accuracy of this tool is verified by comparing it to a Green's function solution. Finally, we demonstrate the potential utility of the method by estimating the seismic response of a large and complex underground highway junction in a given earthquake scenario. Copyright © 2011 John Wiley & Sons, Ltd.
SEPT9_i1 and genomic instability: Mechanistic insights and relevance to tumorigenesis
Authors: Peterson Esther A. , Stanbery Laura , Li Christina , Kocak Hande , Makarova Olga , Petty Elizabeth M. 
Published: September 2011

AbstractSeptins are highly conserved cytoskeletal GTP‐binding proteins implicated in numerous cellular processes from apoptosis to vesicle trafficking. Septins have been associated with leukemia and solid tumor malignancies, including breast, ovarian, and prostate. We previously reported that high SEPT9_i1 expression in human mammary epithelial cell lines (HMECs) led to malignant cellular phenotypes such as increased cell proliferation, invasiveness, motility, and genomic instability. Our goal here was to better understand how SEPT9_i1 expression might contribute to genomic instability and malignant progression. First, we confirmed that even transient expression of SEPT9_i1 was sufficient to increase aneuploidy in HMECs. We then analyzed SEPT9_i1 by immunoprecipitation and immunofluorescence studies and found that SEPT9_i1 interacts with both α and γ tubulin. SEPT9_i1 expressing cells demonstrated dramatic chromosome segregation defects, centrosome amplification and cytokinesis defects, suggesting two possible molecular mechanisms contributing to the development of genomic instability. This suggests that SEPT9_i1 may promote genomic instability through both cytokinesis and mitotic spindle defects which lead to chromosome missegregation. © 2011 Wiley‐Liss, Inc.
The prevalence, incidence and risk factors for delirium in Dutch nursing homes and residential care homes
Authors: Boorsma Marijke , Joling Karlijn J. , Frijters Dinnus H. M. , Ribbe Miel E. , Nijpels Giel , van Hout Hein P. J. 
Published: September 2011

ObjectiveTo estimate and compare the prevalence and incidence of delirium and its risk factors in residents of Dutch nursing homes and residential care homes.MethodsData were extracted from the Long‐Term Care Facility (inter RAI‐LTCF) version of the Resident Assessment Instrument, which was filled in a routine care cohort for a total of 3627 residents. 828 residents of six nursing homes and 1365 residents of 23 residential homes were included in the analyses. Delirium was defined as a positive score on the adjusted Nursing Home–CAM.ResultsThe prevalence of delirium was 8.9% in the nursing homes and 8.2% in the residential homes. The incidence was highest in the nursing homes with 20.7 versus 14.6 per 100 person‐years. Multivariate tests of risk factors for developing delirium included chair restraints (OR 2.3; 95% CI 1.27–4.28), dementia (odds ratio (OR) 3.3; 95% confidence interval (CI) 2.03–5.24) and Parkinson's disease (OR 2.3; 95% CI 0.96–5.63) for residents in nursing homes, and dementia (OR 1.8; 95% CI 1.31–2.55) and fall incidents (OR 1.7; 95% CI 1.20–2.48) for residents in residential care homes.ConclusionThe prevalence and incidence of delirium was high in both nursing homes and residential care homes. More focus on modifiable risk factors such as the use of restraints in nursing homes and fall incidents in residential care homes may help to prevent delirium. Copyright © 2011 John Wiley & Sons, Ltd.
Engagement in leisure activities and benzodiazepine use in a French community‐dwelling elderly population
Authors: Bazin Fabienne , Noize Pernelle , Dartigues Jean‐François , Ritchie Karen Anne , Tavernier Beatrice , Moore Nicholas , Pariente Antoine , Fourrier‐Reglat Annie 
Published: September 2011

ObjectiveThe prevalence of benzodiazepine use among community‐dwelling older persons varies between 10% and 30%. The aim of this study was to explore the association between leisure activities and the use of benzodiazepine among older persons living at home.MethodsThe study population included 4848 persons aged 65 years and over living in either of two French cities. Information was collected from a questionnaire administered to the respondents by trained psychologists during face‐to‐face interviews at home and from a self‐administered questionnaire. Baseline examination included socio‐demographic characteristics, drug use and leisure activities. We classified as benzodiazepine users subjects who reported use of at least one benzodiazepine during the month preceding the interview. The association between the use of benzodiazepine and leisure activities was assessed by logistic regression adjusted on known potential confounders.ResultsMore than 18% of participants reported use of at least one benzodiazepine. The adjusted odds ratio (OR) of benzodiazepine use associated with no or lower participation versus participation in the following activities were as follows: OR = 1.31 (95% confidence interval (CI): 1.09 to 1.58) for mental activity; OR = 1.50 (CI: 1.12 to 2.03) for physical activity; OR = 1.28 (CI: 1.05 to 1.55) for productive activity and OR = 0.82 (CI: 0.69 to 0.97) for recreational activity.ConclusionLow engagement in stimulating activities and high engagement in sedentary activities were associated with recent benzodiazepine use. Copyright © 2011 John Wiley & Sons, Ltd.
The role of the anterior cingulate cortex in emotional response inhibition
Authors: Albert Jacobo , López‐Martín Sara , Tapia Manuel , Montoya Daniel , Carretié Luis 
Published: September 2011

AbstractAlthough the involvement of the anterior cingulate cortex (ACC) in emotional response inhibition is well established, there are several outstanding issues about the nature of this involvement that are not well understood. The present study aimed to examine the precise contribution of the ACC to emotion‐modulated response inhibition by capitalizing on fine temporal resolution of the event‐related potentials (ERPs) and the recent advances in source localization. To this end, participants (N = 30) performed an indirect affective Go/Nogo task (i.e., unrelated to the emotional content of stimulation) that required the inhibition of a motor response to three types of visual stimuli: arousing negative (A−), neutral (N), and arousing positive (A+). Behavioral data revealed that participants made more commission errors to A+ than to N and A−. Electrophysiological data showed that a specific region of the ACC at the intersection of its dorsal and rostral subdivisions was significantly involved in the interaction between emotional processing and motor inhibition. Specifically, activity reflecting this interaction was observed in the P3 (but not in the N2) time range, and was greater during the inhibition of responses to A+ than to N and A−. Additionally, regression analyses showed that inhibition‐related activity within this ACC region was associated with the emotional content of the stimuli (its activity increased as stimulus valence was more positive), and also with behavioral performance (both with reaction times and commission errors). The present results provide additional data for understanding how, when, and where emotion interacts with response inhibition within the ACC. Hum Brain Mapp 33:2147–2160, 2012. © 2011 Wiley Periodicals, Inc.
Disease and genetic contributions toward local tissue volume disturbances in schizophrenia: A tensor‐based morphometry study
Authors: Yang Yaling , Nuechterlein Keith H. , Phillips Owen R. , Gutman Boris , Kurth Florian , Dinov Ivo , Thompson Paul M. , Asarnow Robert F , Toga Arthur W. , Narr Katherine L. 
Published: September 2011

AbstractStructural brain deficits, especially frontotemporal volume reduction and ventricular enlargement, have been repeatedly reported in patients with schizophrenia. However, it remains unclear whether brain structural deformations may be attributable to disease‐related or genetic factors. In this study, the structural magnetic resonance imaging data of 48 adult‐onset schizophrenia patients, 65 first‐degree nonpsychotic relatives of schizophrenia patients, 27 community comparison (CC) probands, and 73 CC relatives were examined using tensor‐based morphometry (TBM) to isolate global and localized differences in tissue volume across the entire brain between groups. We found brain tissue contractions most prominently in frontal and temporal regions and expansions in the putamen/pallidum, and lateral and third ventricles in schizophrenia patients when compared with unrelated CC probands. Results were similar, though less prominent when patients were compared with their nonpsychotic relatives. Structural deformations observed in unaffected patient relatives compared to age‐similar CC relatives were suggestive of schizophrenia‐related genetic liability and were pronounced in the putamen/pallidum and medial temporal regions. Schizophrenia and genetic liability effects for the putamen/pallidum were confirmed by regions‐of‐interest analysis. In conclusion, TBM findings complement reports of frontal, temporal, and ventricular dysmorphology in schizophrenia and further indicate that putamen/pallidum enlargements, originally linked mainly with medication exposure in early studies, also reflect a genetic predisposition for schizophrenia. Thus, brain deformation profiles revealed in this study may help to clarify the role of specific genetic or environmental risk factors toward altered brain morphology in schizophrenia. Hum Brain Mapp 33:2081–2091, 2012. © 2011 Wiley Periodicals, Inc.
Effect of characteristic lengths of electron, ion, and gas diffusion on electrode performance and electrochemical reaction area in a solid oxide fuel cell
Authors: Konno Akio , Iwai Hiroshi , Saito Motohiro , Yoshida Hideo 
Published: September 2011

AbstractA precise evaluation of the active reaction zone in the electrodes is important to design an effective solid oxide fuel cell (SOFC). A scale analysis and one‐dimensional numerical simulations are conducted to obtain a better understanding of the electrochemical reaction zone in a SOFC anode. In the scale analysis, the characteristic lengths of the electron, oxide ion, and gas transports are evaluated from their conservation equations. Relative comparisons of the characteristic lengths show that the transport phenomena in the SOFC anode are primarily governed by the oxide‐ion conduction under standard operating conditions. The gas diffusion may affect the extent and the location of the active reaction zone at high temperature and/or low reaction gas concentration conditions. The one‐dimensional numerical simulations for an anode provided detailed information such as the electric potential of electron‐ and ion‐conducting phases, the gas concentration, and local charge‐transfer current distributions. It is found that the electrochemical reaction actively occurs in the vicinity of the anode–electrolyte interface. The effective thickness increases as the characteristic length of the ion conduction is increased resulting in better power generation performance. The effective thickness is also increased when the gas‐diffusion length is short. The cell performance is, however, lowered in this case because the low gas diffusivity yields the increase of the ohmic loss of ion conduction as well as the concentration overpotential. © 2011 Wiley Periodicals, Inc. Heat Trans Asian Res; Published online in Wiley Online Library (wileyonlinelibrary.com/journal/htj). DOI 10.1002/htj.20373