bluuebunny/crossref_metadata_2025_split huggingface dataset:
Fabrication of Carbohydrate Surfaces by Using Nonderivatised Oligosaccharides, and their Application to Measuring the Assembly of Sugar–Protein Complexes
Authors: Popplewell Jonathan F. , Swann Marcus J. , Ahmed Yassir , Turnbull Jerry E. , Fernig David G. 
Published: April 2009

AbstractThis way up. Dual polarisation interferometry was used to design and characterise a surface on which the orientation and density of immobilised carbohydrates was suitable for studying their interactions with proteins. Lactoferrin was shown to adopt two orientations: “end‐on” or “side‐on”, while for FGF‐2 a single monolayer of protein was observed. The new surface can be used to elucidate the binding of proteins to carbohydrates and the geometry of the complexes, a frequently controversial area.magnified imageSurface‐based tools, such as microarrays and optical biosensors, are being increasingly applied to the analysis of carbohydrate–protein interactions. A key to these developments is the presentation of the carbohydrate to the protein target. Dual polarisation interferometry (DPI) is a surface‐based technique that permits the real‐time measurement of the changes in thickness, refractive index and mass of adsorbates 100 nm thick or less on the surface of a functionalised waveguide. DPI has been used to design and characterise a surface on which the orientation and density of the immobilised carbohydrates is suitable for studying their interactions with proteins and where nonspecific binding is reduced to less than 5 % of total binding. A thiol‐functionalised surface was derivatised with a heterobifunctional crosslinker to yield a hydrazide surface. This was treated with oligosaccharides, derived from keratan sulfate (KS) chondroitin sulfate (CS) and heparin, that possess a reducing end. To block the unreacted hydrazide groups, the surface was treated with an aldehyde‐functionalised PEG. The heparin DP‐10 surfaces were then used to determine the performance of the immobilised DP‐10 with respect to binding of two well‐characterised proteins, lactoferrin (Lf) and fibroblast growth factor‐2. The results show that Lf could adopt two different orientations, at high protein loadings the protein layer thickness corresponded to an “end‐on” orientation of Lf, whilst rinsing with buffer saw the Lf molecules adopt a “side‐on” configuration. In the case of FGF‐2, a single monolayer of protein bound to DP‐10 was observed. These results demonstrate that the new surface can be used to resolve key questions relating to the binding of proteins to carbohydrates, including, when used in DPI, the resolution of the geometry of complexes, an area that is frequently controversial.
New Approach for Local Structure Analysis of the Tyrosine Domain in Proteins by Using a Site‐Specific and Polarity‐Sensitive Fluorescent Probe
Authors: Chen Suming , Li Xiaohua , Ma Huimin 
Published: April 2009

AbstractDesigner label: A newly developed polarity‐sensitive fluorescent probe (DBHA) was combined with a tyrosine‐specific labelling method that uses transition metal catalysis, and was successfully used in local structural analysis of the Tyr108 domain in Cu/Zn superoxide dismutase (SOD; see scheme). The strategy presented here provides a new approach for studying the local polarity and conformation changes of this tyrosine domain in SOD under acid or heat denaturation conditions.magnified imageThe design and synthesis of a novel long‐wavelength polarity‐sensitive fluorescence probe, 6‐[9‐(diethylamino)‐5‐oxo‐5 H‐benzo[α]phenoxazin‐2‐yloxy]hex‐2‐enyl acetate, for the selective modification of tyrosine residues with the goal of providing local information on tyrosine domains in proteins, is reported. This probe comprises a polarity‐sensitive Nile red fluorophore and an active π‐allyl group that can form π‐allylpalladium complexes and react selectively with tyrosine residues. The probe has the following features: 1) it has a long‐wavelength emission of >550 nm, thanks to which interference from short‐wavelength fluorescence from common biological matrixes can be avoided; 2) the maximum emission wavelength is sensitive only to polarity and not to pH or temperature; this allows the accurate determination of local polarity; and 3) it is a neutral, uncharged molecule, and does not disturb the overall charge of the labelled protein. With this probe the polarity and conformation changes of the Tyr108 domain in native and in acid‐ and heat‐denatured bovine Cu/Zn superoxide dismutase were detected for the first time. It was found that the polarity of the Tyr108 domain hardly alters on acid denaturation between pH 4 and 9. However, heat denaturation caused the Tyr108 domain to be more hydrophobic, and was accompanied by an irreversible aggregation of the protein. In addition, the probe‐binding experiments revealed that the surface of the protein becomes more hydrophobic after thermal denaturation; this can be ascribed to the formation of the more hydrophobic aggregates. This strategy might provide a general approach for studying the local environment changes of tyrosine domains in proteins under acid or heat denaturation conditions.
Insights into Sequence–Activity Relationships amongst Baeyer–Villiger Monooxygenases as Revealed by the Intragenomic Complement of Enzymes from Rhodococcus jostii RHA1
Authors: Szolkowy Claudia , Eltis Lindsay D. , Bruce Neil C. , Grogan Gideon 
Published: April 2009

AbstractThe Rhodococcus jostii RHA1 genome encodes a number of enzymes that can be exploited as biocatalysts. Study of the substrate spectrum and enantioselectivity of Baeyer–Villiger monooxygenases from R. jostii allowed the identification of short amino acid sequences specific to groups displaying certain catalytic characteristics. The gel illustrates the substrate acceptance spectra and selectivities of the different proteins.magnified imageMicrobial genome sequences are providing a wealth of information on new enzymes that have considerable potential as biocatalysts. The recently sequenced genome of Rhodococcus jostii RHA1, for example, has revealed an impressive array of catabolic enzymes, including many putative Baeyer–Villiger monooxygenases (BVMOs). We have cloned 23 target BVMO sequences from the genome of R. jostii RHA1 and heterologously expressed 13 of these as soluble proteins to unearth new substrate specificities and selectivities. Whole‐cell biocatalysts expressing the genes were screened against seven different test substrates. Each of these catalysts displayed activity toward at least three ketones. We observed a remarkable diversity of both regio‐ and enantioselectivity among the BVMOs from R. jostii RHA1 for the transformation of two chiral substrates, with some enzymes displaying high enantioselectivity for the isomers of 2‐methylcyclopentanone. With the notable exception of the product of gene ro03437, named MO14, the biocatalysts' sequences correlated well with their respective activities and selectivities. This correlation allowed the identification of sequence motifs specific to subgroups of the BVMOs from R. jostii and other organisms. Overall, the data improve predictive models of BVMO activity from sequence and suggest new avenues to pursue in engineering these enzymes.
Synthesis and Structure of α/δ‐Hybrid Peptides—Access to Novel Helix Patterns in Foldamers
Authors: Sharma Gangavaram V. M. , Babu Bommagani Shoban , Ramakrishna Kallaganti V. S. , Nagendar Pendem , Kunwar Ajit C. , Schramm Peter , Baldauf Carsten , Hofmann Hans‐Jörg 
Published: April 2009

AbstractOrigami peptides: A novel class of foldamers consisting of α/δ‐hybrid peptides has been investigated theoretically and experimentally by exploiting the rigidity of the side chain of a new δ‐amino acid prepared from D‐glucose and D‐xylose with a furanose side chain (see figure).magnified imageStimulated by an overview on all periodic folding patterns of α/δ‐hybrid peptides with 1:1 alternating backbone provided by ab initio molecular orbital theory, the first representatives of this foldamer class were synthesized connecting novel C‐linked carbo‐δ‐amino acid constituents and L‐Ala. In agreement with theoretical predictions, extensive NMR spectroscopic analyses confirm the formation of new motifs of 13/11‐mixed helical patterns in these peptides supported by the rigidity of the D‐xylose side chain in the selected δ‐amino acid constituents. Relationships between possible helix types in α/δ‐hybrid peptides and their counterparts in other 1:1 hybrid peptide classes and native α‐peptides are discussed; these indicate the high potential of these foldamers to mimic native peptide secondary structures. The design of α/δ‐hybrid peptides provides an opportunity to expand the domain of foldamers and allows the introduction of desired functionalities through the α‐amino acid constituents.
Control of the Supramolecular Excimer Formation of Thioflavin T within a Cucurbit[8]uril Host: A Fluorescence On/Off Mechanism
Authors: Mohanty Jyotirmayee , Dutta Choudhury Sharmistha , Upadhyaya Hari P. , Bhasikuttan Achikanath C. , Pal Haridas 
Published: April 2009

AbstractOn or off? A new excimer band at λ=570 nm was visualized during the noncovalent host–guest interaction between thioflavin T (ThT) and cucurbit[8]uril (CB8). Controlled dissociation of this assembly in the presence of Ca2+ was demonstrated as an on/off fluorescence switch (see picture).magnified image
Consecutive Three‐Component Synthesis of Ynones by Decarbonylative Sonogashira Coupling
Authors: Merkul Eugen , Oeser Thomas , Müller Thomas J. J. 
Published: April 2009

AbstractMild conditions! Decarbonylative carbonylation by consecutive glyoxylation of indoles, 7‐aza‐indoles, or pyrroles with oxalyl chloride and subsequent Pd/Cu‐catalyzed decarbonylative alkynylation with terminal alkynes furnishes alkynones under very mild conditions. 4‐(Indol‐3‐yl)‐, 4‐(7‐aza‐indol‐3‐yl)‐, and 4‐(pyrrol‐2‐yl)‐2‐amino pyrimidines can be readily obtained in a concise two‐step synthesis starting from N‐substituted indoles, 7‐aza‐indoles, or pyrroles (see scheme).magnified image
Conformationally Complex π‐Conjugated Molecular and Polymeric Materials: New Challenges for Organic Synthesis
Authors: Guthrie Daryl A. , Tovar John D. 
Published: April 2009

AbstractNo protection? A unique, albeit fairly specialized, example of what promises to be a useful retrosynthetic consideration for those designing complex π‐molecular systems, namely, using conformation as a protecting group to shield a given reactive site in lieu of protecting‐group manipulation, is described (see scheme).magnified imageConformational influences profoundly impact the performance of organic electronic materials and the reactivity of organic molecules. We recently found that the expected former consideration was uniquely accompanied by the latter. This report describes a surprisingly regioselective bromination that suggests the general use of conformation as a protecting group during complex molecule synthesis.
The Electrochemistry of Carbon Nanotubes: Fundamentals and Applications
Authors: Pumera Martin 
Published: April 2009

AbstractElectrocatalytic or not? Carbon nanotubes (CNTs; see figure) are at the forefront of electrochemical research, but care should be taken when describing the electrochemistry, which is often caused by defects or impurities. A discussion on this aspect of their chemistry as well as important applications of CNTs for sensing and energy storage are presented.magnified imageCarbon nanotubes (CNTs) are in the forefront of electrochemical research. It has become clear that an understanding of the fundamental reasons for the electrochemical activity of CNTs is essential for further progress in the field. This review provides a critical discussion of the fundamental reasons behind the electrochemical and “electrocatalytic” activity of CNTs as well as on important applications of CNTs for sensing, biosensing, and energy storage systems.
ChemInform Abstract: Transport Properties and Lithium Insertion Study in the p‐Type Semi‐Conductors AgCuO2 and AgCu0.5Mn0.5O2.
Authors: Sauvage F. , Munoz‐Rojas D. , Poeppelmeier K. R. , Casan‐Pastor N. 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Phase Diagram Investigations of the Ni—Sn—Bi System.
Authors: Vassilev G. P. , Lilova K. I. , Gachon J.‐C. 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Facile Sonochemical Synthesis and Photoluminescent Properties of Lanthanide Orthophosphate Nanoparticles.
Authors: Yu Cuicui , Yu Min , Li Chunxia , Liu Xiaoming , Yang Jun , Yang Piaoping , Lin Jun 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Solvothermal Crystal Growth of CuSbQ2 (Q: S, Se) and the Correlation Between Macroscopic Morphology and Microscopic Structure.
Authors: Zhou Jian , Bian Guo‐Qing , Zhu Qin‐Yu , Zhang Yong , Li Chun‐Ying , Dai Jie 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Construction of Chiral Quaternary Carbon Centers via Palladium‐Catalyzed Asymmetric Deconjugative Allylation.
Authors: Yamamoto Hiroki , Oonishi Yoshihiro , Sato Yoshihiro 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Divergent Enantioselective Pathways in the Catalytic Asymmetric Addition of Diethylzinc to Aldehydes in the Presence and Absence of Titanium Tetraisopropoxide.
Authors: Dean Melissa A. , Hitchcock Shawn R. 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Reduction of Carbon—Carbon Double Bonds Using Organocatalytically Generated Diimide.
Authors: Smit Christian , Fraaije Marco W. , Minnaard Adriaan J. 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Novel Triphenylarsinyl‐Functionalized N‐Heterocyclic Carbene Ligands in Palladium‐Catalyzed C—C Coupling Reactions.
Authors: Stiemke Frank , Gjikaj Mimoza , Kaufmann Dieter E. 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A New Strategy for Chemoselective O‐Acylation of β‐Mercapto Alcohols via Alkylsilyl and Stannyl Protection.
Authors: Maheswara Muchchintala , Kim Mirae , Yun Sun‐Ju , Ju Jung Jin , Do Jung Yun 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Degradable Dendrimers Divergently Synthesized via Click Chemistry.
Authors: Kohman Richie E. , Zimmerman Steven C. 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Catalyst‐Free Aldol Additions of 1,3‐Dicarbonyl Compounds.
Authors: Rohr Kerstin , Mahrwald Rainer 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Simple, Efficient and Versatile Synthesis of Primary gem‐Dihydroperoxides from Aldehydes and Hydrogen Peroxide.
Authors: Bunge Alexander , Hamann Hans‐Juergen , Liebscher Juergen 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Intermolecular Reaction of Internal Alkynes and Imines: Propargyl Tosylates as Key Partners in a Gold‐Catalyzed [4 + 1] Unusual Cyclization Leading to Cyclopent‐2‐enimines.
Authors: Suarez‐Pantiga Samuel , Rubio Eduardo , Alvarez‐Rua Carmen , Gonzalez Jose M. 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Highly Diastereoselective Zn/SnCl2‐Mediated gem‐Difluoroallylation of Chiral Hydrazones.
Authors: Yue Xuyi , Zhang Xingang , Qing Feng‐Ling 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Aminolysis of Epoxides in Ionic Liquid 1‐Ethylpyridinium Trifluoroacetates as Green and Efficient Reaction Medium.
Authors: Malhotra Sanjay V. , Andal Richard P. , Kumar Vineet 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Alkylpalladium N‐Heterocyclic Carbene Complexes: Synthesis, Reactivity, and Catalytic Properties.
Authors: Esposito Oriana , Gois Pedro M. P. , de K. Lewis Alexandra K. , Caddick Stephen , Cloke F. Geoffrey N. , Hitchcock Peter B. 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Synthesis of Camphorsulfonamide‐Based Quinoline Ligands and Their N‐Oxides: First Use in the Enantioselective Addition of Organozinc Reagents to Aldehydes.
Authors: Martinez Ricardo , Zoli Luca , Cozzi Pier Giorgio , Ramon Diego J. , Yus Miguel 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A Novel and Efficient Procedure for the Preparation of Allylic Alcohols from α,β‐Unsaturated Carboxylic Esters Using LiAlH4/BnCl.
Authors: Wang Xiaolong , Li Xiaodong , Xue Jijun , Zhao Yuling , Zhang Yumei 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Catalytic Asymmetric Synthesis of (‐)‐Ritodrine Hydrochloride (V) via Silyl Enol Ether Amination Using Dirhodium(II) Tetrakis [Tetrafluorophthaloyl‐(S)‐tert‐leucinate].
Authors: Tanaka Masahiko , Nakamura Seiichi , Anada Masahiro , Hashimoto Shunichi 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Enantioselective Organo‐Singly Occupied Molecular Orbital Catalysis: The Carbo‐oxidation of Styrenes.
Authors: Graham Thomas H. , Jones Casey M. , Jui Nathan T. , MacMillan David W. C. 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: 2‐(N‐Hydroxylamino) Sulfone Synthesis by Indium—Iodine‐Triggered Aza‐Michael Type Addition of Nitroarenes to Vinyl Sulfones.
Authors: Lee Sun Jung , Lee Jung June , Kim Chong‐Hyeak , Jun Young Moo , Lee Byung Min , Kim Byeong Hyo 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: An Efficient Guanylation of Aromatic Amines Catalyzed by Samarium Diiodide.
Authors: Zhu Xuehua , Xu Fan , Shen Qi 
Published: April 2009

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 200 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.