bluuebunny/crossref_metadata_2025_split huggingface dataset:
Calcium oxalate crystals as a cause of multiple punctate echogenic foci in benign thyroid lesions
Authors: Suzuki Ayana , Hirokawa Mitsuyoshi , Otsuka Izumi , Miyauchi Akira , Akamizu Takashi 
Published: April 2024

Abstract
Purpose
Multiple punctate echogenic foci (MPEF) on thyroid ultrasonography reflects psammoma bodies in papillary thyroid carcinomas. However, MPEF is also observed in benign thyroid lesions. The aim of this study was to determine the origin of MPEF in patients with benign thyroid lesions.

Methods
We enrolled 26 patients with Graves’ disease (GD) and 24 with follicular nodular disease (FND) who exhibited MPEF and underwent surgery. As controls, we enrolled 40 patients with GD and 32 with FND, but without MPEF, who underwent surgery.

Results
MPEF was observed in both lobes in 80.8% of GDs with MPEF, but was limited to a single lobe in the remaining cases. MPEF was diffusely distributed in 72.3% of the cases and focally distributed in the remaining cases. On ultrasonography, most (92.3%) FNDs with MPEF were solid lesions, and seven nodules (26.9%) were interpreted as intermediate suspicion and their frequencies were higher than in those without MPEF (p < 0.01). Microscopically, calcium oxalate (CaOx) crystals were observed more frequently in GDs and FNDs with MPEF (100% and 88.5%, respectively) than in those without MPEF (p < 0.001). These differences were particularly significant for CaOx crystals > 100 μm. In GD cases, large CaOx crystals were observed more frequently in the lobes with MPEF than in those without (p < 0.05). No psammoma bodies were present in any of the cases.

Conclusion
Appearance of MPEF in GDs and FNDs is not because of psammoma bodies; it is attributable to CaOx crystals larger than 100 μm. Therefore, MPEF is not an indicator of malignancy.
A Comparison of Internal Mandrel Designs for Rotary Draw Bend Forming of Carbon-fibre/Thermoplastic (PA6) Tubular Structures
Authors: Li Mengyuan , Stokes-Griffin Chris , Holmes John , Sommacal Silvano , Compston Paul 
Published: April 2024

AbstractCarbon fibre reinforced thermoplastic tubular structures can be post-formed into desired curvatures via rotary draw bending (RDB) at elevated temperatures. During this process, a rigid internal mandrel is required to support the walls of the tubes to maintain their ovality and minimise unwanted geometrical distortions. This paper investigates four internal mandrel designs for post-forming carbon fiber reinforced polyamide 6 (CF/PA6) thermoplastic tubes. Mandrel designs include silicone rod, bullet, wire, and coil spring, were evaluated through RDB-forming experiments with [± 60°]4 CF/PA6 tubes formed to 90° bends. The designs were evaluated for their effectiveness on minimising distortions resulted from induced stresses during post-forming by measuring the post-formed tube diameter and extrados strains. The mandrel designs were also evaluated for their usability when integrated into the RDB process. Results from optical measurements and micro-computed tomography showed the spring mandrel outperformed others, producing tubes with the least geometrical distortions and no defects during the forming process. As compared to other designs, the spring mandrel is a reusable unibody design that is easy to assemble and remove from the tubes.
Comparing the effectiveness of real-time PCRs to simultaneously detect and identify viable Globodera pallida and G. rostochiensis
Authors: Lombard L. , Dekker-Nooren C. C. E. M. , Wuijster B. , van Kessel S. P. , van Duivenbode I. , van Bruggen A. S. , van Heese E. Y. J. 
Published: April 2024

AbstractRecently, the Commission Implementation Regulation (CIR) 2022/1192 was published by the European Union, prescribing regulations on eradication measures for quarantine potato cyst nematodes, including mandated assays for species identification. The CIR 2022/1192 provides a derogation on the use of Beniers et al. (2014) real-time PCR as the validation process was still ongoing. In this study, the validation of a modified version of the Beniers real-time PCR was carried out, following EPPO Standard PM 7/98. Furthermore, the performance was compared to the conventional PCR of Bulman & Marshall (1997) and the Gamel et al. (2017) real-time PCR, both DNA based, which are mandated by CIR 2022/1192. Additionally, the effect of cyst viability was compared with the two real-time PCR assays. The modified Beniers real-time PCR met all validation performance criteria for both target Globodera pallida and G. rostochiensis. The viability assays showed that the DNA based Gamel real-time PCR provided positive results for non-viable, non-infectious cysts, in contrast to the modified RNA based Beniers real-time PCR. Based on the results of this study, the modified Beniers qualifies for the detection and identification of viable Globodera pallida and G. rostochiensis and performs at least equally as well as the other two PCR assays. To encourage the development and improvement of diagnostic protocols, it is suggested that diagnostic laboratories performing official surveys are allowed to use internationally recognized diagnostic protocols when validated in-house according to EPPO standards and accredited by the EU bodies (EPPO, 2021).
The renoprotective activity of amikacin–gamma-amino butyric acid–chitosan nanoparticles: a comparative study
Authors: Madbouly Neveen , Ooda Adham , Nabil Ahmed , Nasser Areej , Ahmed Esraa , Ali Fatma , Mohamed Fatma , Faried Habiba , Badran Mai , Ahmed Mariam , Ibrahim Mariam , Rasmy Mariam , Saleeb Martina , Riad Vereena , Ibrahim Yousr , Farid Alyaa 
Published: April 2024

AbstractThe development of nanoparticles (NPs) with active components with upgraded stability, and prolonged release helps in enhanced tissue regeneration. In addition, NPs are feasible strategy to boost antibiotic effectiveness and reduce drug side effects. Our study focuses on the use of amikacin (AMK) and gamma amino butyric acid (GABA) unloaded combinations or loaded on chitosan nanoparticles (CSNPs) for kidney protection. The AMK–GABA–CSNPs were prepared with the ionic gelation method, the morphology was studied using transmission electron microscopy (TEM), zetasizer and the Fourier transform-infrared spectroscopy (FT-IR) spectrum of the synthesized NPs was observed. The average size of AMK–GABA–CSNPs was 77.5 ± 16.5 nm. Zeta potential was + 38.94 ± 2.65 mV. AMK–GABA–CSNPs revealed significant in vitro antioxidant, anti-coagulation, non-hemolytic properties and good cell compatibility. To compare the effects of the unloaded AMK–GABA combination and AMK–GABA–CSNPs on the renal tissue, 42 healthy Sprague–Dawley rats were divided into seven groups. G1: normal control (NC), normal saline; G2: low-dose nephrotoxic group (LDN), AMK (20 mg/kg/day; i.p.); G3: unloaded AMK (20 mg/kg/day; i.p.) and GABA (50 mg/kg/day; i.p.); G4: AMK–GABA–CSNPs (20 mg/kg/day; i.p.); G5: high-dose nephrotoxic group (HDN), AMK (30 mg/kg/day; i.p.); G6: unloaded AMK (30 mg/kg/day; i.p.) and GABA (50 mg/kg/day; i.p.) and G7: AMK–GABA–CSNPs (30 mg/kg/day; i.p.). The results showed that AMK–GABA–CSNPs formulation is superior to unloaded AMK–GABA combination as it ameliorated kidney functions, oxidative stress and displayed a significant homeostatic role via suppression of inflammatory cytokines of Th1, Th2 and Th17 types. Hence, AMK–GABA–CSNPs could afford a potential nano-based therapeutic formula for the management of AMK-nephrotoxicity.
Enhancing Chlamydomonas reinhardtii growth and metabolite biosynthesis using organic dyes as spectral converters
Authors: Ramanna Luveshan , Nasr Mahmoud , Rawat Ismail , Bux Faizal 
Published: April 2024

AbstractThe availability of light and its efficient utilisation is a major limiting factor in large scale algal cultures. While algae predominantly use the blue and red spectral regions, a significant amount of incoming light energy remains untapped outside these ranges. Converting this unused light into usable wavelengths could enhance photosynthesis. This study aimed to identify the optimal spectral converter among Lumogen Red (LR), Rhodamine 8G (R8G), and Lumogen Yellow (LY), providing efficient light utilisation for algal cultivation.Chlamydomonas reinhardtiiwas cultivated in double-jacketed cylindrical photo-bioreactors (PBRs) using 30% UV-aand 5% UV-bfluorescent tubes. The R8G dye displayed a slight wavelength shift with a sharp peak at about 498 nm. The LY dye demonstrated several sharp peaks at the green and blue light spectra evident of photo-degradation. The LR dye maintained better photo-stability compared to R8G and LY. Carbohydrate, lipid and protein were produced early, whereas biomass increased after day 4, as a result of photo-acclimation. The LR dye converted and emitted 3.17 × 10-19J photon-1, enhancing biomass production and increasing photochemical energy utilisation [Y(II)] while decreasing regulated energy dissipation [Y(NPQ)]. Pigment biosynthesis was initially increased and then reduced to counteract heightened irradiation as a means of photo-protection. LR had a 1.6- and 2.9-fold up-regulation of the RuBisCo gene expression. The dye-based system correlated with improved growth, lipid, protein, carbohydrate, and pigment production. The dye-based wavelength conversion system was interlinked to the sustainable development goals addressing environmental, economic, and social aspects.
Anthropomorphising Machines and Computerising Minds: The Crosswiring of Languages between Artificial Intelligence and Brain & Cognitive Sciences
Authors: Floridi Luciano , Nobre Anna C 
Published: April 2024

AbstractThe article discusses the process of “conceptual borrowing”, according to which, when a new discipline emerges, it develops its technical vocabulary also by appropriating terms from other neighbouring disciplines. The phenomenon is likened to Carl Schmitt’s observation that modern political concepts have theological roots. The authors argue that, through extensive conceptual borrowing, AI has ended up describing computers anthropomorphically, as computational brains with psychological properties, while brain and cognitive sciences have ended up describing brains and minds computationally and informationally, as biological computers. The crosswiring between the technical languages of these disciplines is not merely metaphorical but can lead to confusion, and damaging assumptions and consequences. The article ends on an optimistic note about the self-adjusting nature of technical meanings in language and the ability to leave misleading conceptual baggage behind when confronted with advancement in understanding and factual knowledge.
Tire–road contact modelling for multibody simulations with regularised road and enhanced UA tire models
Authors: Millan P. , Ambrósio J. 
Published: April 2024

Abstract
Multibody simulations play an important role in the study of the dynamic behaviour of road vehicles, being one of the key aspects the modelling of the tire-road interaction. The tire-road contact modelling is required to detect the contact between the tire and road and to obtain the tire contact forces from the tire-road relative motion. The first part of the problem is solved by a contact detection algorithm, whereas the second part is handled by a tire model. In both cases, the trade-off between accuracy and computational cost needs to be addressed in the road dynamics scenario. For the contact detection problem, this work proposes a methodology in which the road is described by a mesh of triangles while the tire is described by a toroid. The triangular mesh road input can be directly extracted from any CAD model. To avoid the geometric discontinuities due to the transition between triangular patches, a set of auxiliary points is used to obtain a continuous variation of the road surface normal, based on the method proposed by Rill (Multibody Syst. Dyn. 45:131–153, 2019. In the process of contact detection, all kinematic quantities required by the tire-road contact model are evaluated. The tire–road forces are evaluated using the enhanced UA tire model, which is a numerically stable and efficient development of the original tire model proposed by Gim and Nikravesh (Int. J. Veh. Des. 12, 1991; Int. J. Veh. Des. 12:19–39, 1991; Int. J. Veh. Des. 12:217–228, 1991). Both the contact and tire models have smooth transitions between their internal transitions, thereby reducing the need for the variable-step ODE integrators to unnecessarily decrease time steps. The advances on contact detection and the tire–road contact models, proposed here, are demonstrated with a set of road vehicle simulation scenarios in which the influence of various modelling parameters on the stability and efficiency of the simulations is addressed. The novelties of the work consist not only in the demonstration of the enhanced UA tire model in practical situations but also in the description of the new tire–road contact methodology that employs a simple description of the road geometry, to achieve smooth tire–road contact forces.
Numerical integrator for highly oscillatory differential equations based on the Neumann series
Authors: Perczyński Rafał , Madejski Grzegorz 
Published: April 2024

Abstract
We propose a third-order numerical integrator based on the Neumann series and the Filon quadrature, designed mainly for highly oscillatory partial differential equations. The method can be applied to equations that exhibit small or moderate oscillations; however, counter-intuitively, large oscillations increase the accuracy of the scheme. With the proposed approach, the convergence order of the method can be easily improved. Error analysis of the method is also performed. We consider linear evolution equations involving first- and second-time derivatives that feature elliptic differential operators, such as the heat equation or the wave equation. Numerical experiments consider the case in which the space dimension is greater than one and confirm the theoretical study.
Global state of the art of teaching life cycle assessment in higher education
Authors: Viere Tobias , Lehmann Joshua , Miao Zoe Chunyu , Harding Kevin , Strothmann Philip , Weyand Steffi , Wright Laurie , Chitaka Takunda Y. , Sonnemann Guido 
Published: April 2024

Abstract
Purpose
Globally, there is an increased demand for education on life cycle assessment (LCA). In response, there has been an increase in course availability, but also a lack of clarity on the comprehensiveness of these offerings and the resulting student competencies.

Methods
A global survey was conducted to obtain empirical evidence on teaching LCA. The survey explored the availability of LCA courses globally and the depth of the teaching, including expected core competencies and related teaching and learning workloads. A purposive sampling strategy was adopted wherein eligible participants were approached by the researchers.

Results and discussion
According to the survey, annually, over 10,000 students participate in more than 200 LCA courses. The results reflected the interdisciplinary nature of LCA with courses being taught across different disciplines, including engineering, chemical sciences, and economics. Estimated workload demands for achieving different competency levels were significantly lower than those estimated by an expert panel before. This may be attributed in part to respondents not accounting for the full workload beyond classroom interactions. Nonetheless, workload demands increased with competency levels.

Conclusions and recommendations
The results emphasize the need for a common understanding of LCA teaching with regard to content, literacy levels, and competencies to avoid false expectations of the labor and research markets in terms of available expertise. Therefore, LCA curriculum development and program planning remain significant challenges and essential tasks for the global LCA community.
The role of interventional radiology and molecular imaging for near infrared photoimmunotherapy
Authors: Kobayashi Hisataka , Choyke Peter L. 
Published: April 2024

AbstractNear infrared photoimmunotherapy (NIR-PIT) is a recently approved cancer therapy for recurrent head and neck cancer. It involves the intravenous administration of an antibody-photoabsorber (IRDye700DX: IR700) conjugate (APC) to target cancer cells, followed 24 h later by exposure to near infrared light to activate cell-specific cytotoxicity. NIR-PIT selectively targets cancer cells for destruction and activates a strong anticancer host immunity. The fluorescent signal emitted by IR700 enables the visualization of the APC in vivo using fluorescence imaging. Similarly, the activation of IR700 during therapy can be monitored by loss of fluorescence. NIR-PIT can be used with a variety of antibodies and therefore, a variety of cancer types. However, in most cases, NIR-PIT requires direct light exposure only achieved with interstitial diffuser light fibers that are placed with image-guided interventional needle insertion. In addition, the unique nature of NIR-PIT cell death, means that metabolic molecular imaging techniques such as PET and diffusion MRI can be used to assess therapeutic outcomes. This mini-review focuses on the potential implications of NIR-PIT for interventional radiology and therapeutic monitoring.
Apnea Testing on Conventional Mechanical Ventilation During Brain Death Evaluation
Authors: Merchant Rameez Ali , Ahmad Shahid Nafees , Haddix Bradley , Williamson Craig Andrew , Jacobs Teresa Lee , Singh Tarun Deep , Nguyen Andrew M. , Rajajee Venkatakrishna 
Published: April 2024

Abstract
Introduction
The use of continuous positive airway pressure has been shown to improve the tolerance of the apnea test, a critical component of brain death evaluation. The ability to deactivate the apnea backup setting has made apnea testing possible using several conventional mechanical ventilators. Our goal was to evaluate the safety and efficacy of apnea testing performed on mechanical ventilation, compared with the oxygen insufflation technique, for the determination of brain death.

Methods
This was a retrospective study. In 2016, our institution approved a change in policy to permit apnea testing on conventional mechanical ventilation. We examined the records of consecutive adults who underwent apnea testing as part of the brain death evaluation process between 2016 and 2022. Using an apnea test technique was decided at the discretion of the attending physician. Outcomes were successful apnea test and the occurrence of patient instability during the test. This included oxygen desaturation (SpO2) < 90%, hypotension (mean arterial pressure < 65 mm Hg despite titration of vasopressor), cardiac arrhythmia, pneumothorax, and cardiac arrest.

Results
Ninety-two adult patients underwent apnea testing during the study period: 58 (63%) with mechanical ventilation, 32 (35%) with oxygen insufflation, and 2 (2%) lacked documentation of technique. Apnea tests could not be completed successfully in 3 of 92 (3%) patients—two patients undergoing the oxygen insufflation technique (one patient with hypoxemia and one patient with hypotension) and one patient on mechanical ventilation (aborted for hemodynamic instability). Hypoxemia occurred in 4 of 32 (12.5%) patients with oxygen insufflation and in zero patients on mechanical ventilation (p = 0.01). Hypotension occurred during 3 of 58 (5%) tests with mechanical ventilation and 4 of 32 (12.5%) tests with oxygen insufflation (p = 0.24). In multivariate analysis, the use of oxygen insufflation was an independent predictor of patient instability during the apnea test (odds ratio 37.74, 95% confidence interval 2.74–520.14).

Conclusions
Apnea testing on conventional mechanical ventilation is feasible and offers several potential advantages over other techniques.
Case report: Mutation evolution in a patient with TdT positive high grade B cell lymphoma with MYC and BCL2 rearrangements following the treatment of concurrent follicular lymphoma and diffuse large B-cell lymphoma
Authors: Zhang Fen , Chen Yu , Cui Qian , Ge Yan , Liu Yanhui 
Published: April 2024

Abstract
Background
Concurrent follicular lymphoma (FL) and diffuse large B-cell lymphoma (DLBCL)was reported in some studies, while the diagnosis of TdT (terminal deoxynucleotydil transferase) positive high grade B cell lymphoma (HGBL) with MYC and BCL2 rearrangements (“double hit”) transformed from FL/DLBCL has been rarely reported. Herein, we described the clinical features and mutation profiles of a case diagnosed with TdT positive “double hit” HGBL following the treatment of FL/DLBCL.

Case presentation
This is a 43-year-old Chinese man who was diagnosed with low grade FL (account for 80%) combined with DLBCL (20%) at a stage of IVB. The patient presented with BCL2/IGH translocation without MYC rearrangement, as well as the expressions of CD20, CD19, CD10 and BCL2 at the initial diagnosis of FL/DLBCL. MYC rearrangement and TdT expression occurred after the treatment. The targeted sequencing revealed mutations in KMT2D, FOXO1, CREBBP, ATM, STAT6, BCL7A, DDX3X, MUC4, FGFR3, ARID5B, DDX11 and PRKCSH genes were the co-mutations shared by the FL/DLBCL and TdT positive “double hit” HGBL, while CCND3, BIRC6, ROBO1 and CHEK2 mutations specifically occurred after the treatment. The overall survival time was 37.8 and 17.8 months after the initial diagnosis of FL/DLBCL and TdT positive “double hit” HGBL, respectively.

Conclusion
This study reports a rare case of TdT positive “double hit” HGBL following the treatment of concurrent FL/DLBCL and highlights the mutation characteristics. Collectively, this study will help enrich the knowledge of TdT positive “double hit” HGBL transformed from FL/DLBCL.
Fractional nonlinear heat equations and characterizations of some function spaces in terms of fractional Gauss–Weierstrass semi–groups
Authors: Baaske Franka , Schmeißer Hans-Jürgen , Triebel Hans 
Published: April 2024

Abstract
We present a new proof of the caloric smoothing related to the fractional Gauss–Weierstrass semi–group in Triebel-Lizorkin spaces. This property will be used to prove existence and uniqueness of mild and strong solutions of the Cauchy problem for a fractional nonlinear heat equation.
Mixed Hodge structures on character varieties of nilpotent groups
Authors: Florentino Carlos , Lawton Sean , Silva Jaime 
Published: April 2024

Abstract
Let

$$\textsf{Hom}^{0}(\Gamma ,G)$$

Hom
0

(
Γ
,
G
)

be the connected component of the identity of the variety of representations of a finitely generated nilpotent group

$$\Gamma $$

Γ

into a connected reductive complex affine algebraic group G. We determine the mixed Hodge structure on the representation variety

$$\textsf{Hom}^{0}(\Gamma ,G)$$

Hom
0

(
Γ
,
G
)

and on the character variety

$$\textsf{Hom}^{0}(\Gamma ,G)/\!\!/G$$

Hom
0

(
Γ
,
G
)

/

/
G

. We obtain explicit formulae (both closed and recursive) for the mixed Hodge polynomial of these representation and character varieties.