bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pendidikan Holistik Islam Perspektif KH. Abdul Wahid Hasyim
Authors: Fadillah Muti'ah , Kartika Resti Okvani 
Published: June 2024

This research aims to explore K.H. Abdul Wahid Hasyim's thoughts about Islamic holistic education. Islamic holistic education is an educational approach that includes spiritual, intellectual, emotional and physical aspects in overall individual development. This writing applies qualitative techniques through library research. The data presented in this paper originated from the thoughts of KH. Abdul Wahid Hasyim spread in many books and articles. The findings from this study are that K.H. Abdul Wahid Hasyim has forward thinking ideas in about Islamic holistic education. His thoughts on character education are also relevant in the context of contemporary Islamic education. In this holistic education, Wahid Hasyim emphasizes five things, including overall individual development, integration of Islamic knowledge and values, contextual learning, strengthening spirituality and building a just society. His thoughts can be a reference in developing holistic Islamic education, which includes the development of all aspects of an individual's life
Nutritional Content and Acceptability of Vegetable Sausage as an Alternative Source of Protein and Fiber
Authors: Kunaepah Uun , Sulistiyono Priyo , Estuti Wiwit 
Published: June 2024

Nutrition problems in Indonesia are still relatively high. SSGI results (2021) show that malnutrition is 17.1% and stunting is 24.4%. Lack of food consumption, especially protein, is one of the causes. Utilization of tofu dregs is still very low, as is banana blossom. Tofu dregs and banana blossoms have quite a high protein and fiber content. Tofu dregs and banana blossoms have the potential to become a high-nutrient food product that is suitable for consumption and has economic value. This research is a follow-up research from organoleptic tests, finding that vegetable sausages have organoleptic qualities that are no less than commercial sausages. This research aims to conduct laboratory tests for protein and fiber content and tests for the acceptability of vegetable sausages. This type of research is experimental, by design of two groups with control. Test acceptability with the method Visual Comstock by looking at leftovers. The acceptability test was conducted on 51 children in grades IV and V at SDN Pamitran-Cirebon who met the screening criteria. The treatment group consisted of 24 children, and the control group consisted of 27 children. Sample assignment was carried out randomly. Analysis of crude protein content using the IK-03/LAKP/SOP.10 method. Analysis of oil fiber content IK-13/LAKP/SOP.10. Analysis of the difference in receptivity test using the t-test of unpaired data. The research results showed that the protein content of vegetable sausages was (7.54%), slightly lower than commercial sausages (9.29%). The crude fiber content of vegetable sausages (3.48%) is higher than commercial sausages (2.10%). Vegetable sausages have the same acceptability as commercial sausages, with a good acceptability value of 100%. It has been proven that vegetable sausages can be used as an alternative food source of high protein and fiber at a relatively cheaper price.
Exceeding Humanism
Authors: Horváth Eszter 
Published: June 2024

The paper introduces Jean-Luc Nancy and Michel Serres as two philosophers of the perpetual and spontaneous rebeginning of philosophy: always in the process of inventing itself, branching off, mutating, and escaping, spurring the spirit towards continuous renewal. Nancy and Serres, two whimsically energetic, creative, innovative thinkers, both take into account, even rely in their philosophies on the parasitic procedures that tend to reorganize the structures of western culture. In this paper, these parasitic procedures come to the fore, as one of the most important aspects of innovation and creation.
Behind-the-scenes: vers l’égalité de genre au sein des présidences syndicales locales?
Authors: Bernard Pelletier Marie-Pier , Le Capitaine Catherine 
Published: June 2024

Cet article porte sur l’expérience des présidences impliquées dans les organisations syndicales locales au regard de l'égalité de genre. À partir d’une étude de cas qualitative menée auprès des membres de comités syndicaux de la condition féminine et de présidences syndicales locales du secteur de l’éducation du Québec, notre recherche fait valoir une percée sans précédent des femmes siégeant comme présidentes locales. Néanmoins, l’existence d’une « triple tâche », la persistance de stéréotypes de genre et d’une culture syndicale masculine, le manque de formation et de mentorat ainsi que le recours à la sollicitation nuisent à l’atteinte de l’égalité de genre. Nos résultats de recherche font aussi ressortir une faible représentation de la diversité des identités sociales au sein de l’organisation syndicale étudiée.RésuméCet article porte sur l’expérience des présidences impliquées dans les organisations syndicales locales au regard de l'égalité de genre. Dans un contexte où l’équité, la diversité et l’inclusion de la main-d’oeuvre sont plus que jamais d’actualité, cet écrit contribue aux réflexions entourant le déficit démocratique de la représentation des femmes au sein des organisations syndicales. Les résultats reposent sur une étude de cas qualitative menée auprès des membres de deux comités syndicaux de la condition féminine et de 16 présidences syndicales locales travaillant dans le secteur de l’éducation du Québec. En recueillant le point de vue de ces femmes et de ces hommes, notre recherche fait valoir une avancée quant à la place longtemps délaissée des femmes siégeant localement comme présidentes au sein de l’organisation syndicale étudiée. Ce sont les efforts déployés par les syndicats eux-mêmes qui ont permis peu à peu aux femmes d’accéder à l’univers syndical traditionnellement masculin. Derrière cette percée sans précédent, il en ressort néanmoins que cette avancée demeure lacunaire pour atteindre l’égalité de genre. Outre un certain déficit numérique des femmes qui résiste au temps qui passe, la difficulté d’articuler une « triple tâche », la persistance de stéréotypes de genre, la prégnance d’une culture syndicale masculine, le manque de formation et de mentorat ainsi que le recours à la sollicitation sont des freins importants qui empêchent les femmes d’accéder aux postes syndicaux locaux. De plus, la représentation de la diversité des identités sociales (âge, situation familiale, race, etc.) est encore peu présente au palier décisionnel local de l’organisation syndicale étudiée, et ce, même parmi les femmes élues. La diversité et la représentation de l’égalité de genre demeurent des défis à prioriser pour assurer une organisation syndicale pérenne et démocratique.
On Sums of Squares Involving Integer Sequence: \(\sum_{r=1}^n w_r^2+\frac{n}{3} d^2=3\left(\frac{n d^2}{3}+\sum_{r=1}^{\frac{n}{3}} w_{3 r-1}^2\right)\)
Authors: Mude Lao Hussein , Ndung’u Kinyanjui Jeremiah , Kayiita Zachary Kaunda 
Published: June 2024

Let wr be a given integer sequence in arithmetic progression with a common difference d. The study of diophantine equations, which are polynomial equations seeking integer solutions, has been a very interesting journey in the field of number theory. Historically, these equations have attracted the attention of many mathematicians due to their intrinsic challenges and their significance in understanding the properties of integers. In this current study, we examine a diophantine equation relating the sum of squared integers from specific sequences to a variable d: In particular, the diophantine equation \(\sum_{r=1}^n w_r^2+\frac{n}{3} d^2=3\left(\frac{n d^2}{3}+\sum_{r=1}^{\frac{n}{3}} w_{3 r-1}^2\right)\) is introduced and partially characterized. The objective is to determine the conditions under which integer solutions for (wr,d) exist within this diophantine equation.The methodology of solving this problem entails, decomposing polynomials, factorizing polynomials, and exploring the solution set of the given equation.
Adverse Events of the Use of SGLT2 Inhibitors in Patients with Heart Failure: A Systematic Review and Meta-analysis
Authors: Britto Mariana Feldner de , Oliveira Ana Luiza Miranda de , Simões Ricardo , Guimarães Nathalia Sernizon , Polese Janaine Cunha , Souza Alessandra Hubner de 
Published: June 2024

Background: Sodium-glucose cotransporter 2 (iSGLT2) inhibitors are a class of medications used in the treatment of Heart Failure (HF) to prevent the development and worsening of the disease. Despite the benefits reported in the literature, it is essential to investigate its adverse effects.
Objective: To evaluate the occurrence of hypotension, volume depletion and acute kidney injury in patients with HF with reduced (HFrEF) and preserved (HFpEF) ejection fraction using iSGLT2.
Methodology: Systematic review and meta-analysis previously registered in PROSPERO (CRD42022365684) and carried out in the PubMed, EBSCO and LILACS databases. The risk of bias analysis was performed using the Cochrane risk-of-bias tool for randomized trials. RevMan 3.4.1 was used to perform the data meta-analysis.
Results: Of the 9,474 studies found, ten were included in the review. A risk of hypotension was observed in patients with HF using iSGLT2 (RR: 1.15; 95% CI 1.01 – 1.30; p=0.03; I2= 0%), but there was no risk statistically significant occurrence of volume depletion and acute kidney injury (RR: 1.12; 95% CI 1.95 – 1.33; p=0.17; I2= 0%; RR: 0.85; 95% CI 0 .69 – 1.06; p=0.14;
Conclusion: In therapy with iSGLT2, an increased risk of adverse events of hypotension was observed, but no increased risk of volume depletion and acute kidney injury was observed. Despite the 15% reduction in relative risk in the outcome of acute kidney injury, the results suggest a renal benefit.
Preparation of Genomic DNA from Mammalian Sperm
Authors: Weyrich Alexandra 
Published: April 2012

AbstractSemen consists of spermatozoa and the seminal fluid, also called seminal plasma. This fluid is important for the survival of the spermatozoa, but may decrease the purity and thus quality of the DNA due to its fructose and protein content. In this protocol, spermatozoa are washed with ethanol to remove the fluid. The spermatozoa themselves are protected by a membrane rich in disulfide bonds, which impede cell lysis and thus hamper DNA isolation. To break disulfide bonds a strong antioxidant, such as dithiothreitol (DDT), is necessary. Similar to other protocols for DNA isolation from other mammalian tissues, proteinase K and SDS are included in the lysis reaction. After lysis of the sperm cells, the DNA is precipitated using ethanol and then redissolved in TE‐buffer or ddH2O. Curr. Protoc. Mol. Biol. 98:2.13.1‐2.13.3. © 2012 by John Wiley & Sons, Inc.
Native Elongating Transcript Sequencing (NET‐seq)
Authors: Churchman L. Stirling , Weissman Jonathan S. 
Published: April 2012

AbstractAdvances in sequencing technology have led to the development of many high‐resolution methodologies that observe genomic activity and gene expression. This unit describes such an approach, native elongating transcript sequencing (NET‐seq), which reveals the density of RNA polymerase across the Saccharomyces cerevisiae genome with single‐nucleotide resolution. A procedure for capturing nascent RNA transcripts directly from live cells through their association with the DNA‐RNA‐RNAP ternary complex is described. A protocol to create DNA libraries from the nascent RNA, allowing the identity and abundance of the 3′ end of purified transcripts to be revealed by next generation sequencing, is also provided. By deep sequencing this DNA library, a quantitative measure of RNAP density with single‐nucleotide precision is obtained. The quantitative nature of the NET‐seq dataset relies on the high efficiency of many steps in the protocol. The steps that are most critical are described with suggestions for monitoring their success. Curr. Protoc. Mol. Biol. 98:14.4.1‐14.4.17. © 2012 by John Wiley & Sons, Inc.
Target‐Induced Natural Killer Cell Loss as a Measure of NK Cell Responses
Authors: Warren Hilary 
Published: April 2012

AbstractNatural killer (NK) cells are an important effector cell of innate immunity. Their interaction with susceptible target cells triggers NK cell cytotoxicity and the release of cytokines. Immunofluorescence flow cytometry–based assays are now the preferred methods for measuring NK cell responses. For these assays, assessment is made on NK cells (CD3–CD56+ CD16+) within the viable lymphocyte gate, defined by the parameters of size (FSC) and granularity (SSC). Accordingly, NK cells that have not dissociated from target cells at the time of measurement, or that have undergone target cell–induced apoptosis, are excluded from the viable lymphocyte gate and therefore from analysis. This unit describes a protocol for assessing NK cell function in response to target cells (both natural killing and antibody‐dependent cell cytotoxicity) based on the loss of NK cells from the lymphocyte gate. This target‐induced NK loss (TINKL) should provide a sensitive measure of NK cell responses in a clinical laboratory setting. Curr. Protoc. Immunol. 97:14.29.1‐14.29.15. © 2012 by John Wiley & Sons, Inc.
Differentiation of Mast Cells from Embryonic Stem Cells
Authors: Kovarova Martina , Koller Beverly 
Published: April 2012

AbstractIn this unit, we describe a simple coculture‐free method for obtaining mast cells from mouse and human embryonic stem (ES) cells. Much of our knowledge regarding the mechanisms by which mast cells are activated comes from studies of mouse bone marrow–derived mast cells. Studies of human mast cells have been hampered by the limited sources from which they can be cultured, the difficulty in introducing specific genetic changes into these cells, and differences between established cultures that reflect the unique genetic makeup of the tissue donor. Derivation of mast cells from embryonic stem cells addresses these limitations. ES‐derived mast cells can be generated in numbers sufficient for studies of the pathways involved in mast cell effector functions. These ES cell–derived mast cells respond to antigens and other stimuli by releasing histamine, cytokines, lipids, and other bioactive mediators. The derivation of human mast cells from ES cells carrying mutations introduced by homologous recombination should provide a novel means of testing the function of genes in both the development and the effector functions of mast cells. Curr. Protoc. Immunol. 97:22F.10.1‐22F.10.16. © 2012 by John Wiley & Sons, Inc.
Prevalence of measles, mumps, rubella, and varicella susceptibility among health science students in a University in India
Authors: Arunkumar G. , Vandana K.E. , Sathiakumar Nalini 
Published: April 2012

AbstractBackgroundHealth science students (HSS) are at increased risk of contracting and transmitting viral diseases such as measles, mumps, rubella, and varicella (chickenpox). This study was undertaken to determine the prevalence of susceptibility of HSS to these infections.MethodsUsing a cross‐sectional design, 790 HSS of Manipal University, Manipal, India, answered a questionnaire and provided a blood sample which was tested for specific IgG antibodies to measles, mumps, rubella and varicella by ELISA (Enzygnost®).ResultsThe study group was comprised of medical (53.9%), nursing (16.6%), and allied health (29.5%) students. Among the overall group (n = 790), the prevalence of serological susceptibility to measles, mumps, rubella, and varicella were 9.5%, 32.0%, 16.6%, and 25.8%, respectively. Among the subgroup of vaccinated subjects, susceptibility to measles, mumps, rubella, and varicella were 7.9%, 34.7%, 10.7%, and 35.2%, respectively.ConclusionHSS susceptible to measles, mumps, rubella, and/or varicella are at risk of acquiring these diseases during their training period. In addition, they may be a potential source for nosocomial transmission posing a risk to immunocompromised patients. Hence, in the Indian setting, HSS should be immunized against measles, mumps, rubella, and varicella at the time of joining the medical school. Am. J. Ind. Med. 56:58–64, 2013. © 2012 Wiley Periodicals, Inc.
Association of CRHR1 and CRHR2 with major depressive disorder and panic disorder in a Japanese population
Authors: Ishitobi Yoshinobu , Nakayama Shinya , Yamaguchi Kana , Kanehisa Masayuki , Higuma Haruka , Maruyama Yoshihiro , Ninomiya Taiga , Okamoto Shizuko , Tanaka Yoshihiro , Tsuru Jusen , Hanada Hiroaki , Isogawa Koichi , Akiyoshi Jotaro 
Published: April 2012

AbstractMajor depressive disorder (MDD) and panic disorder (PD) are common and disabling medical disorders with stress and genetic components. Dysregulation of the stress response of the hypothalamic–pituitary–adrenal axis, including the corticotrophin‐releasing hormone (CRH) signaling via primary receptors (CRHR1 and CRHR2), is considered to play a major role for onset and recurrence in MDD and PD. To confirm the association of CRHR1 and CRHR2 with MDD and PD, we investigated 12 single nucleotide polymorphisms (SNPs) (rs4076452, rs7209436, rs110402, rs242924, rs242940, and rs173365 for CRHR1 and rs4722999, rs3779250, rs2267710, rs1076292, rs2284217, and rs226771 for CRHR2) in MDD patients (n = 173), PD patients (n = 180), and healthy controls (n = 285). The SNP rs110402 and rs242924 in the CRHR1 gene and the rs3779250 in the CRHR2 gene were associated with MDD. The SNP rs242924 in the CRHR1 gene was also associated with PD. The T‐A‐T‐G‐G haplotype consisting of rs7209436 and rs173365 in CRHR1 was positively associated with MDD. The T‐A haplotype consisting of rs7209436 and rs110402 in CRHR1 was positively associated with MDD. The C‐C haplotype consisting of rs4722999 and rs37790 in CRHR1 was associated with PD. These results provide support for an association of CRHR1 and CRHR2 with MDD and PD. © 2012 Wiley Periodicals, Inc.
Laryngeal support device enhances the learning of laryngeal anatomy and voice physiology
Authors: Curcio Daniella Franco , Behlau Mara , Barros Mirna Duarte , Smith Ricardo Luiz 
Published: April 2012

AbstractMultidisciplinary cooperation in health care requires a solid knowledge in the basic sciences for a common ground of communication. In speech pathology, these fundamentals improve the accuracy of descriptive diagnoses and support the development of new therapeutic techniques and strategies. The aim of this study is to briefly discuss the benefits of hands‐on education on laryngeal anatomy and voice physiology in Brazilian graduate programs in speech pathology, as well as to describe a simple prototype that can be used as a useful educational tool for this purpose. The laryngeal anatomic support device was designed to provide a vertical frame to hold human or mammalian larynges with no preservation treatment, with the goal of allowing good visualization of the vocal folds during artificial phonation. The device was designed to provide the user the ability to manipulate the soft and cartilaginous structures of the larynx with near‐natural biomechanical properties. The description of the project is detailed to allow the reproduction of this simple and inexpensive device. It may be used as an experimental feature in a variety of settings, from high‐school programs to experimental research methods, and may suit a wide array of different educational models. Anat Sci Educ. © 2012 American Association of Anatomists.
Automatic detection of melanoma progression by histological analysis of secondary sites
Authors: Orlov Nikita V. , Weeraratna Ashani T. , Hewitt Stephen M. , Coletta Christopher E. , Delaney John D. , Mark Eckley D. , Shamir Lior , Goldberg Ilya G. 
Published: April 2012

AbstractWe present results from machine classification of melanoma biopsies sectioned and stained with hematoxylin/eosin (H&E) on tissue microarrays (TMA). The four stages of melanoma progression were represented by seven tissue types, including benign nevus, primary tumors with radial and vertical growth patterns (stage I) and four secondary metastatic tumors: subcutaneous (stage II), lymph node (stage III), gastrointestinal and soft tissue (stage IV). Our experiment setup comprised 14,208 image samples based on 164 TMA cores. In our experiments, we constructed an HE color space by digitally deconvolving the RGB images into separate H (hematoxylin) and E (eosin) channels. We also compared three different classifiers: Weighted Neighbor Distance (WND), Radial Basis Functions (RBF), and k‐Nearest Neighbors (kNN). We found that the HE color space consistently outperformed other color spaces with all three classifiers, while the different classifiers did not have as large of an effect on accuracy. This showed that a more physiologically relevant representation of color can have a larger effect on correct image interpretation than downstream processing steps. We were able to correctly classify individual fields of view with an average of 96% accuracy when randomly splitting the dataset into training and test fields. We also obtained a classification accuracy of 100% when testing entire cores that were not previously used in training (four random trials with one test core for each of 7 classes, 28 tests total). Because each core corresponded to a different patient, this test more closely mimics a clinically relevant setting where new patients are evaluated based on training with previous cases. The analysis method used in this study contains no parameters or adjustments that are specific to melanoma morphology, suggesting it can be used for analyzing other tissues and phenotypes, as well as potentially different image modalities and contrast techniques. Published 2012 Wiley Periodicals, Inc.
The correlation between pain‐related behaviour and spinal microgliosis in four distinct models of peripheral neuropathy
Authors: Blackbeard J. , Wallace V.C.J. , O'Dea K.P. , Hasnie F. , Segerdahl A. , Pheby T. , Field M.J. , Takata M. , Rice A.S.C. 
Published: April 2012

AbstractBackgroundPeripheral nerve injury is associated with a spinal microglial response that has been correlated with the development of behaviours reflective of neuropathic pain.MethodsTo examine whether this phenomenon is generalizable to neuropathic pain of non‐traumatic aetiology, this study investigated the association between spinal microgliosis and behavioural measures of neuropathic hypersensitivity and pain‐related anxiety behaviour in four distinct rat models of peripheral neuropathic pain. These were traumatic neuropathy [L5 spinal nerve transection (SNT)], HIV‐related neuropathies (either treatment with the antiretroviral drug Zalcitabine (ddC) or combination of perineural exposure to the HIV‐gp120 protein and ddC treatment) and varicella zoster virus (VZV) infection.Results and ConclusionPersistent mechanical hypersensitivity developed in all ‘neuropathic’ rats. However, spinal microgliosis, as measured by increased CD11b/c immunohistochemical staining and increased numbers of cells expressing CD11b measured by flow cytometry, was evident in the SNT and to a lesser extent in the HIV neuropathy models but not the VZV model. These results suggest that behavioural hypersensitivity and thigmotaxis can only be linked to a microglial response in certain models of neuropathy.