bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of hub genes and potential molecular mechanisms in MSS/MSI classifier primary colorectal cancer based on multiple datasets
Authors: Qiao Xia , Zhang Xu 
Published: June 2024

Abstract

Objective
MSI has a better prognosis than MSS in colorectal cancer patients, and the main objective of this study was to screen MSI/MSS primary colorectal cancer differentially expressed molecules by bioinformatics.
Material and methods
Two gene expression datasets (GSE13294 and GSE13067) were downloaded from GEO, and differential expressed genes (DEGs) were analyzed using GEO2R. Gene Ontology and Kyoto Encyclopedia of Genomes and Gene Set Enrichment Analysis were achieved using the differentially expressed genes. Furthermore, a Protein-Protein Interaction Networks (PPI) was constructed to screen for significant modules and identify hub genes. The hub genes were analyzed using GEPIA in colorectal cancer. Expression of hub genes in clinical samples was visualized by the online Human Protein Atlas (HPA).
Results
A total of 266 common DEGs were identified in MSS primary colorectal cancer compared with MSI primary colorectal cancer. Among these, 178 DEGs were upregulated and 87 DEGs were downregulated. Enrichment analysis showed that set enrichment was associated with response to response to mechanical stimulus, regulation of cellular response to stress, g protein coupled receptor binding, and other processes. A total of 5 hub genes was identified by cytoHubba: HNRNPL, RBM39, HNRNPH1, TRA2A, SRSF6. GEPIA software online analysis, 5 hub gene expression in colorectal cancer survival curve did not have significant differences. The expression of RBM39 was significantly different in different stages of colorectal cancer. Then, HPA online database of results show that the five hub protein expression varied widely in CRC patients.
Conclusion
The hub genes, such as HNRNPH1and RBM39, and the spliceosome resulting from DEGs, which may provide novel insights and evidence for the future diagnosis and targeted therapy of MSS/MSI PCRC.
Experimental research on damage characteristics of red sandstone under the combined action of temperature, water and stress
Authors: Bao Xiankai , Qiao Jianlong , Yu Chaoyun , Tian Baolong , Wang Lingyu , Zhang Xiaofan 
Published: June 2024

Abstract

In order to study the damage properties of red sandstone under combined action of temperature, water and stress, the uniaxial compression test was carried out for red sandstone after immersing in water for 0.5 hours, 1 hour and 3 hours at 25℃, 55℃, 85℃ and 95℃, the mechanical properties, characteristic stress points, acoustic emission RA-AF parameters and failure forms of red sandstone were analyzed, the damage constitutive equation was established, and the damage properties of red sandstone were studied. The results showed: (1) Temperature could significantly improve the saturated water content percentage of red sandstone. With the increase of temperature, the saturated water content percentage showed a trend of increase first and then decrease, and was the largest at 55℃; with the increase of temperature, the mechanical parameters (peak intensity, elasticity modulus, and stress at start point of expansion) decreased first and then increased, and was the smallest at 55℃; (2) The ratio of crack initiation stress to peak stress (σci/σf) increased first and then decreased at the same time of immersing in water as temperature increased, and decreased first and then increased as the time of immersing in water increased at the same temperature; (3) Acoustic emission RA-AF signals are primarily found in the stable and unstable crack extension stages, with most signals concentrated in the tensile areas. As immersion time increases, there is a gradual decrease in the total number of signals, while the proportion of signals in the shearing areas significantly increases. This is relatively consistent with the patterns of failure observed in red sandstone experiments; (4) The damage of temperature, water and stress on red sandstone is categorized into initial damage and load damage, and the damage constitutive equation of red sandstone under the joint action of temperature-water-load is established, which is applicable to red sandstone in water-saturated state below 100℃, The research results can provide certain theoretical references for practical engineering.
Flexible DC distribution network fault protection method based on transient characteristics differences of fault current
Authors: Zeng Zhi-hui , Li Jia-yin , Wei Yan-fang , Wang Xiao-wei , Zheng Ying-ying 
Published: June 2024

Abstract

To improve the rapidity and accuracy of fault detection in multi-terminal flexible DC distribution networks with modular multilevel converters. A protection method based on the transient characteristic difference of fault current is proposed. First, the parameters of the complete ensemble empirical mode decomposition with adaptive noise (CEEMDAN) are optimized using the Aquila Optimizer algorithm. So that, the feature extraction effect is optimal. Secondly, the distinction in the magnitude of internal and external fault currents is deduced through detailed fault characterization. Thus, the energy moment eigenvalue of the fault current is used to construct the internal and external fault criterion. Then, depending on the distinction between the faulty and sound pole currents during internal fault. The ratio of the singular value of the positive and negative pole currents is taken to create the differentiation criterion of faulty pole. This protection scheme can reliably and sensitively recognize various types of short-circuit faults by only using DC line single-end current signals. Finally, the validity and robustness of this method are confirmed by simulating and analyzing under different working conditions.
Expression of apoptosis-related proteins in injured muscle tissue of rats before and after death
Authors: Li Shoutian , Zhong Yunlong , Yu Anxiao , Zhang Zhongqing , Tan Li , Liu Yong 
Published: June 2024

Abstract

Objective To study the expression of Bax, Bcl-2 and Caspase-3, three apoptosis-related proteins after skeletal muscle injury in rats, and explore their application value in distinguishing antemortem and postmortem injuries.
Methods 42 healthy adult Sprague-Dawley rats were randomly divided into control group, antemortem injury group and postmortem injury group. The gastrocnemius muscle injury of the right hind limb of rats was made. The feasibility of the model was verified by HE staining. The mRNA expression was detected by Q-PCR. The expression of Bax, Bcl-2, and Caspase-3 proteins was detected through Western Blot, the data were centralized and standardized respectively, and orthogonal partial least square-discriminate analysis (OPLS -DA) of antemortem and postmortem injuries was established.
Results HE staining cannot accurately distinguish between antemortem and postmortem injuries; The changing trend of Caspase-3 mRNA expression is consistent with the protein expression trend; the protein expression levels of Bax, Bcl-2, and Caspase-3 were centered and standardized and analyzed through OPLS -DA. The model can distinguish antemortem injury or postmortem injury samples.
Conclusion The expression of Bax, Bcl-2 and Caspase-3 proteins in local tissues of rat skeletal muscle injury can be used to differentiate between antemortem and postmortem injuries.
Metabolomic Profiling of COVID-19 Using Serum and Urine Samples in Intensive Care and Medical Ward Cohorts
Authors: Tristán Ana Isabel , Jiménez-Luna Cristina , Abreu Ana Cristina , Arrabal-Campos Francisco Manuel , Salmerón Ana del Mar , Rodríguez Firma Isabel , Maresca Manuel Ángel Rodríguez , García Antonio Bernardino , Melguizo Consolación , Prados José Carlos , Fernández Ignacio 
Published: June 2024

Abstract

The COVID-19 pandemic remains a significant global health threat, with uncertainties persisting regarding the factors determining whether individuals experience mild symptoms, severe conditions, or succumb to the disease. This study presents an NMR metabolomics-based approach, analyzing 80 serum and urine samples from COVID-19 patients (34 intensive care patients and 46 hospitalized patients) and 32 from healthy controls. Our research identifies discriminant metabolites and clinical variables relevant to COVID-19 diagnosis and severity. We propose a three-metabolite diagnostic panel—comprising isoleucine, TMAO, and glucose—that effectively discriminates COVID-19 patients from healthy individuals, achieving high efficiency. Recognizing that serum profiles are more reliable but invasive compared to urine samples, we propose reconstructing serum profiles using urine 1H NMR data. Our robust multi-output regression model demonstrates high accuracy in this reconstruction, and in classifying the converted serum spectroscopic profile. This suggests the feasibility of determining COVID-19 infection and predicting its severity using a non-invasive sample such as urine.
Dietary supplementation with hesperidin increased growth, antioxidant capacity, and transcription of immune-related genes in Litopenaeus vannamei (Boone 1931)
Authors: Arshadi Ali , Azhdari Ashkan 
Published: June 2024

Abstract

We examined the effects of the flavonoid hesperidin on nonspecific immunity gene expression, antioxidant capacity and growth performance of Litopenaeus vannamei. Four isonitrogen-containing (~ 38%) diets were formulated and supplemented with hesperidin at 0 (control; T1), 100 (T2), 200 (T3), and 300 (T4) mg kg− 1. Shrimps (2.45 ± 0.17 g) were placed in 12 circular fiberglass tanks filled with 150 L of seawater, and each feed treatment was done in triplicate. Shrimps were hand-fed three times daily for 8 weeks at 7% of their original biomass. According to the results, the growth rate of shrimps T3 was significantly higher (∼2.83 g) than that of the control. The feed conversion ratio in shrimp fed hesperidin-enriched feeds was better compared to the control group (P < 0.05). Shrimps T3 and T1 showed the highest and lowest plasma biochemical parameters, respectively (P < 0.05), while the other groups had intermediate values. Shrimps T3 and T1 had the highest and lowest plasma phenoloxidase activity, respectively. Catalase activity, glutathione peroxidase activity and superoxide dismutase activity in hepatopancreas were increased by dietary hesperidin supplementation, while malondialdehyde level was significantly decreased by increasing dietary hesperidin level (P < 0.05). The relative abundance of the mRNA transcript of the genes prophenoloxidase, lysozyme, penaeidin-3α and heat shock protein 70 in the hepatopancreas of T3 was significantly higher than in the other groups. Based on the above findings, consumption of aquafeeds containing 200 mg hesperidin kg− 1 can enhance growth performance in L. vannamei juvenile by promoting antioxidant capacity and immune responses.
Probing the distribution of rare earth ions in glass-ceramics by the properties of Er3+ ion probes
Authors: Chen Zhixin , Cui Wenzhe , Yang Ju , Tian Jiayu , Ren Guozhong 
Published: June 2024

Abstract

Glass and glass-ceramic samples with matrix of SiO2-PbF2 system were firstly prepared. The glass was transformed into glass-ceramics by heat treatment, and the effect of different heat treatment times in this process on the microenvironment of rare earth ions into the glass-ceramics was investigated. The proportion of Er3+ entering the glass-ceramics during heat treatment was calculated by using J-O theory combined with absorption spectroscopy using Er3+ as a probe. On the other hand, Er3+ was used as a probe to investigate and analyze the changes of the crystalline phase of the glass-ceramics by combining fluorescence spectra, absorption spectra, and XRD changes. The results show that the XRD patterns indicate that heat treatment of the precursor glass at the transition temperature produces rapid crystalline deposition of PbF2, but most of the rare earth ions are still present in the glass phase. And with the gradual increase of the heat treatment time, the rare earth ions in the glass phase gradually enter into the fluoride nanocrystals, but this process is not synchronized with the crystallization process.
Rare pathogenic structural variants show potential to enhance prostate cancer germline testing for African men
Authors: Hayes Vanessa , Gong Tingting , Jiang Jue , Bornman Riana , Gheybi Kazzem , Stricker Phillip , Weischenfeldt Joachim , Mutambirwa Shingai 
Published: June 2024

Abstract

Prostate cancer (PCa) is highly heritable, with men of African ancestry at greatest risk and associated lethality. Lack of representation in genomic data means germline testing guidelines exclude for African men. Established that structural variations (SVs) are major contributors to human disease and prostate tumourigenesis, their role is under-appreciated in familial and therapeutic testing. Utilising a clinico-methodologically matched African (n = 113) versus European (n = 57) deep-sequenced PCa resource, we interrogated 42,966 high-quality germline SVs using a best-fit pathogenicity prediction workflow. We identified 15 potentially pathogenic SVs representing 12.4% African and 7.0% European patients, of which 72% and 86% met germline testing standard-of-care recommendations, respectively. Notable African-specific loss-of-function gene candidates include DNA damage repair MLH1 and BARD1 and tumour suppressors FOXP1, WASF1 and RB1. Representing only a fraction of the vast African diaspora, this study raises considerations with respect to the contribution of kilo-to-mega-base rare variants to PCa pathogenicity and African associated disparity.
Drug Release and Stability Study of Innovated Losartan Potassium and
Rosuvastatin Calcium Fixed-dose Combination Tablet
Authors: Zeb Mian Inaam , Khan Salimullah , Ataya Farid S. , Batiha Gaber El-Saber , Kashtoh Hamdy , Khan Ajmal , Al-Harrasi Ahmed 
Published: June 2024

Background:
Patient adherence to therapy and compliance is always a challenge for care providers
in the management of chronic disorders with multiple medications.

Objective:
Our study focused on formulating concurrently prescribed ARB (Angiotensin Receptor Blocker),
i.e., losartan potassium, and a cholesterol-lowering statin derivative, i.e., rosuvastatin calcium, in a fixed-dose
combination tablet.

Methods:
The drugs were selected based on the presence of synergism and variation in solubility characteristics.
Trial batches with fixed concentrations of both active pharmaceutical ingredients (APIs) and varying
quantities of different excipients were prepared by dry granulation technique and subjected to different quality
control tests for tablets. Batch F5 was selected on the basis of in-process quality control data for the development
of a drug release protocol. Experimental conditions were optimized. Based on the sink condition, phosphate
buffer (pH 6.8) was selected as the dissolution medium. Simultaneous determination of both APIs in
samples collected at predetermined time intervals was carried out using the RP-HPLC technique with acetonitrile,
methanol, and water (20:25:55 v/v/v) as mobile phase.

Results:
Complete dissolution of both APIs in the FDC tablet was achieved in 45 min in 900 mL of the selected
medium. The in vitro drug release protocol was validated for accuracy and precision without interference
with sample analysis.

Conclusion:
In this study, a validated, accurate, and robust dissolution testing method was developed for the
newly formulated FDC tablet.
Design, Synthesis and Evaluation of Antifungal Activity of Pyrazoleacetamide Derivatives
Authors: Kachi Onkar G. , Pawar Hari R. , Chabukswar Anuruddha R. , Jagdale Swati , Swamy Vishwanath , Vinayak Kadam , Hingane Dattatray , Shinde Mahadev , Pawar Nagesh 
Published: June 2024

Background:
Fungal infections have posed a big challenge in the management of their
treatment. Due to the resistance and toxicity of existing drug molecules in the light of pandemic
infections, like COVID-19, there is an urgent need to find newer derivatives of active molecules,
which can be effective in fungal infections.

Objective:
In the present study, we aimed to design pyrazole derivatives using molecular modeling
studies against target 1EA1 and synthesize 10 molecules of pyrazole derivatives using a multi-step
synthesis approach.

Methods:
Designed pyrazole derivatives were synthesized by conventional organic methods. The
newly synthesized pyrazole molecules were characterized by using FT-IR, 1HNMR, 13CNMR, and
LC-MS techniques. Molecular docking studies were also performed. The antifungal activity of
newly synthesized compounds was assessed in vitro against Candida albicans and Aspergillus niger
using the well plate method.

Results:
Two of the compounds, OK-7 and OK-8, have been found to show significant docking
interaction with target protein 1EA1. These two compounds have also been found to show significant
anti-fungal activity against Candida albicans and Aspergillus nigra when compared to the
standard fluconazole. The Minimum Inhibitory Concentration (MIC) value of these two compounds
has been found to be 50 μg/ml.

Conclusion:
Pyrazole derivatives with -CH3, CH3O-, and -CN groups have been found to be active
against tested fungi and can be further explored for their potential as promising anti-fungal agents
for applications in the field of medicinal chemistry.
Systematic Review and Meta-analysis of the Impact of Metabolic Surgery on Hepatic Stiffness
Authors: Jamialahamdi Tannaz , Kroh Matthew , Ashari Sorour , Almahmeed Wael , Jangjoo Ali , Simental-Mendía Luis E , Salehabadi Sepideh , Al-Rasadi Khalid , Sahebkar Amirhossein 
Published: June 2024

Background:
Bariatric surgery is one of the effective therapeutic options for people
with obesity and obesity-related co-morbidities. In addition to weight-related co-morbid diseases,
including diabetes, hypertension, and hypercholesterolemia, non-alcoholic fatty liver disease
(NAFLD) is common in patients with morbid obesity. Bariatric surgery is one of the therapeutic
options in the management of NAFLD. Hence, this review focused on the potential role of bariatric
surgery on hepatic elasticity measured through shear wave elastography.

Methods:
A systematic literature search was performed, and the studies regarding heterogeneity
were evaluated using the random-effects model.

Results:
The meta-analysis on 6 trials (3-12 months follow-up) including 350 participants showed
a significant reduction of liver elasticity after surgery (WMD: -1.149, 95% CI: -1.767, -0.532, p <
0.001; I2:81.55%).

Conclusion:
Bariatric surgery is associated with decreased liver elasticity. This improvement
could be related to weight loss or other mechanisms of bariatric surgery.
Advancements in GERD Treatment: Exploring Innovative Therapies
Authors: Lunawat Akshay Kumar , Raikwar Sarjana 
Published: June 2024

:
Gastroesophageal Reflux Disease (GERD) stands as a prevalent and impactful gastrointes-tinal disorder, affecting a substantial portion of the global population. Characterised by the chronic backflow of stomach acid into the oesophagus, GERD presents itself with symptoms ranging from the discomfort of heartburn to more severe complications, including esophageal inflammation and respiratory issues [1]. The pervasive nature of this condition underscores the critical need for effective and evolving treatment strategies. As individuals worldwide grapple with the challenges posed by GERD, the quest for advancements in treatment options becomes paramount. The prevalence of GERD extends far beyond its physical manifestations, reaching into the realms of daily life, productivity, and overall well-being. Heartburn, regurgitation, and chest pain, hallmarks of GERD, can disrupt sleep patterns, hinder nutritional habits, and compromise the quality of life of an individual [2].
Moreover, the chronic nature of the disease raises concerns about long-term complications, such as Barrett's oesophagus and an increased risk of esophageal cancer. The burden imposed by GERD is not solely physical; it extends to psychological well-being, influencing factors like stress and anxiety. Recognising the multifaceted impact of GERD underscores the urgency of advancing treatment options to address the diverse needs of those affected [3].
Special Education and Field Education: Literary Review
Authors: Carvalho Renata Natalina , Vasconcellos Talita Silva Perussi 
Published: June 2024

Objective: Recognizing the importance of the rural education process from the perspective of educating people with disabilities, this study sought to understand what Brazilian literature in recent years on the subject offers us.

Theoretical Framework: Seeks to bring legislative evolution in search of knowledge about the transversality of special education.

Method: The bibliographic survey was carried out in the periodic databases CAPES, Scielo and in the productions made available by GEPEC - Group of Studies and Research in Rural Education at UFSCar. In order to analyze the selected research, the method consisted of quantitative and qualitative, dividing the process into categories for verification and discussion.

Results and Discussion: The analysis procedures allowed us to conjecture that studies in the area are scarce and to highlight that the increase in research that focuses on these aspects is important.

Implications of the Research: The research allowed us to understand the scenario of Brazilian studies on special education in the field.

Originality/Value: The study brought contributions to future research, providing an overview of how Brazilian research still needs to answer questions on the topic.
Morphometric Transformations of the Perdizes and Fojo Watersheds in Campos do Jordão, SP, Brazil: Land Use/Land Cover Changes after 12 Years, with Focus on Urbanized Areas
Authors: Fulan Mateus Henrique , Cavalheiro Wanderson Cleiton Schmidt , Santos Junior Nilson Reinaldo Fernandes dos , Souza Renato Francisco da Silva , Santos Miquele Araújo dos , Carneiro Kaline de Almeida Alves , Macedo Rodrigo Santana , Fulan João Ânderson , Vendruscolo Jhony 
Published: June 2024

Objective: The main objective of this study was to compare urbanized areas, supplemented by additional information such as tree vegetation, areas without vegetation, and the presence of agriculture in the Fojo and Perdizes watershed located in Campos do Jordão, SP, Brazil, 12 years after their characterization, using supervised classification methods.

Theoretical Framework: The use of geotechnologies for the assessment of the LULC change allows the holistic visualization of the area, compared to former studies.

Method: The base image for the study was generated through the Semi-Automatic Classification Plugin, with Sentinel-2 satellite spectral data as the base. Land use and cover classifications were conducted through Quantum GIS, with the Orfeo Toolbox plugin as the supervised classification tool.

Results and Discussion: The results obtained reveal significant land use changes in Campos do Jordão, specifically on Fojo watershed, where there was a 48% decrease of urban land use.

Research Implications: The theoretical implications of the research contributes to the future study of other environmental interest areas, allowing the classification of other regions, encouraging future researches of land use and cover changes, influencing other areas such as geoprocessing, urban planning and ecology.

Originality/Value: Usage of modern technologies for the assessment of land use change in an area previously studied can evidentiate recent changes, in this case, the great reduction of urbanized area in Campos do Jordão. The results obtained through the study may influence further scientific research especially for geoprocessing, as new technologies aid a better understanding.