bluuebunny/crossref_metadata_2025_split huggingface dataset:
The analysis of plasma proteomics for hormone receptor-positive HER2-negative breast cancer
Authors: Zhao meimei , jiang yongwei , kong xiaomu , liu yi , gao peng , li mo , zhu haoyan , deng guoxiong , feng ziyi , cao yongtong , ma liang 
Published: May 2024

Abstract

Background

Breast cancer (BC) is the prevailing malignancy among women, exhibiting a discernible escalation in incidence within our nation, hormone receptor-positive (HR+) human epidermal growth factor receptor 2-negative (HER2-) breast cancer is the most common subtype. In this study, we aimed to search for a non-invasive, specific, blood-based biomarker for the early detection of HR+/HER2- breast cancer through proteomic studies.

Methods

To explore new potential plasma biomarkers, we applied data independent acquisition (DIA), a technique that combines liquid chromatography with tandem mass spectrometry to quantify breast cancer-associated plasma protein abundance from a small number of plasma samples.

Results

The proteomes of 30 participants in all cohorts were analyzed using the DIA method, and a total of 517 proteins and 3584 peptides were quantified. We found that there were significant differences in plasma protein expression profiles between breast cancer patients and non-breast cancer patients, and breast cancer was mainly related to lipid metabolism pathways. Finally, the optimal protein combinations for the diagnosis of breast cancer were PON3, IGLV3-10 and IGHV3-73 through multi-model analysis, which had a high prediction accuracy for breast cancer (AUC=0.92), and the model could also distinguish breast cancer from HC (AUC=0.92) and breast cancer from benign breast tumor (AUC=0.91).

Conclusions

The study revealed proteomic signatures of patients with HR+/HER2- breast cancer, identified multiple differential proteins, and identified three plasma proteins as potential diagnostic biomarkers for breast cancer. It provides a reference for the screening of biomarkers for breast cancer.
Alcohol consumption and the incidence of hyperuricemia in Japanese men and women: the ISSA-CKD study
Authors: Ueno Tamami , Kawano Kazumi , Nohara Chihiro , Maki Kaori , Iwanaga Kazuyo , Morinaga Akiko , Funakoshi Shunsuke , Abe Makiko , Satoh Atsushi , Kawazoe Miki , Maeda Toshiki , Yoshimura Chikara , Takahashi Koji , Tada Kazuhiro , Ito Kenji , Yasuno Tetsuhiko , Mukobara Shigeaki , Kawanami Daiji , Masutani Kosuke , Arima Hisatomi 
Published: May 2024

Abstract

The present study investigated the relationship between alcohol consumption and the incidence of hyperuricemia separately in Japanese men and women. A total of 5,153 participants without hyperuricemia at baseline were included in the analysis. Alcohol consumption was classified into non-drinkers, occasional drinkers, and daily drinkers (average alcohol intake <22 g/day, 22-43 g/day, 44-65 g/day, or ≥66 g/day). The outcome was incident hyperuricemia (uric acid >416 μmol/L [7.0 mg/dL]). During the follow-up period, hyperuricemia was detected in 624 participants (496 men and 128 women). Increases in the incidence (per 1,000 person-years) of hyperuricemia were associated with the frequency/amount of alcohol consumed among men (33.7 for non-drinkers, 43.9 for occasional drinkers, 41.7 for <22 g/day, 60.6 for 22-43 g/day, 68.0 for 44-65 g/day, and for 92.7 ≥66 g/day; P<0.001). Similar results were observed among women (6.1 for non-drinkers, 10.3 for occasional drinkers, 8.8 for <22 g/day, 15.7 for 22-43 g/day, and 7.7 for ≥44 g/day; P=0.008). Significant differences were also observed after adjustments for potential confounders (P <0.001; in both men and women). In conclusion, the risk of developing hyperuricemia in the future was associated with the amount of alcohol consumed among Japanese men and women.
Applying standardized patients to augment clinical skills in clinical medical students’ national proficiency test
Authors: Wang Haihong , Lv Hanbing , Shi Shuwen , Yang Jingyu , Zheng Liujuan , Fang Xiangming 
Published: May 2024

Abstract

Purpose: To examine the impact of standardized patient encounters (SPEs) on clinical thinking ability and skills of clinical medical students.
Methods: Fourth-year medical students admitted from 2016 and 2017 received curriculum that was not included SPEs while from 2018 and 2019 started to complete a clinical thinking ability and skills curriculum that was expanded to include SPEs. Learners completed 6 SPEs clinical cases. Faculty observers and standardized patients completed checklists focused on history-taking, counseling, and interpersonal communication, and provided learner feedback after each case. The curriculum was evaluated by comparing clinical skills scores in clinical medical students’ national proficiency test each year. National proficiency test included 6 skills stations: 2 stations assessing history collection, 2 stations assessing physical examination and 2 stations assessing basic clinical skills. Changes in clinical skills scores were assessed.
Results: In the study, there were 757 students admitted from 2106 and 2017 received curriculum that was not included SPEs (SPEs group) and 731 students admitted from 2018 completed the skills curriculum that was expanded to include SPEs (Normal group). Learners’ overall clinical scores improved by 2.8 (confidence interval [CI]: 2.29-3.25; P<0.0001) in SPEs group compared with normal group. Similar improvements in station 2, 3, 4 and 5 scores were occurred in SPEs group.
Conclusions: The combination of SPEs with clinical skills curriculum is effective at improving national proficiency test scores for clinical medical students. The comprehensive curriculum allowed learners to better apply knowledge and practice skills.
The causal relationship between hyperthyroidism, hypothyroidism, and osteoporosis: a Mendelian randomization study
Authors: Duan Wenyu , Hao Liang , Shuang Wang , Xiaolong Fan , Yulu Wang 
Published: May 2024

Abstract

To investigate the association between hyperthyroidism、hypothyroidism and osteoporosis by bidirectional two-sample Mendelian randomization (MR) to clarify the casual relationship. Independent genetic variants for hyperthyroidism、hypothyroidism and osteoporosis were selected as instruments from published genome-wide association studies (GWAS), mainly in European ancestry. Instrumental variables (IVs) associated with hyperthyroidism、hypothyroidism and osteoporosis were extracted separately from the largest GWAS meta-analysis. MR analyses included inverse variance weighting, weighted median estimator, MR Egger regression, and sensitivity analyses with Steiger fltering and MR PRESSO. In the data samples for hyperthyroidism、hypothyroidism and osteoporosis, there was a negative causal relationship between hyperthyroidism and osteoporosis [IVW, OR = 1.001%, 95% CI: 0.999–1.002, ]; while hypothyroidism and osteoporosis [IVW, OR = 2.648%, 95% CI: 2.582–2.715, p < 0.001]. The results of the bidirectional MR Study suggest that hyperthyroidism and hypothyroidism have negative causal effect on Osteoporosis, which provides implications for clinical treatment decisions in patients with Osteoporosis.
Stress distribution characteristics and crack propagation law of fractured hard rock influenced by opening degree
Authors: Jia Baoxin , Liu Fengpu 
Published: May 2024

Abstract

Fracture opening degree and inclination angle are important factors affecting the crack evolution as well as the strength of the fractured rock body. To investigate the mechanical characteristics and cracking behavior of hard rock specimens under the combined influence of opening degree and inclination angle, we conducted uniaxial compression tests and numerical simulations on pre-cracked hard rock specimens. The study shows that as the crack dip angle increases, the initial crack position of hard rock specimens with prefabricated cracks of the same opening shifts from the short-axis end to the long-axis end. Additionally, when the crack opening is less than 1.5mm, the peak strength of the sample initially decreases and then increases with the increase of the crack dip angle. The peak strength of the sample increases as the crack dip angle increases when the crack opening is greater than 1.5mm. On the other hand, when the crack opening is less than 0.4mm, the peak crack initiation stress of the specimen first decreases and then increases with the increase of the crack inclination angle. When the crack opening is greater than 0.4mm, the crack initiation stress of the specimen gradually increases with the increase of the crack inclination angle. During the compression process, the closure of the crack surface weakens the concentration of compressive stress at the end of the prefabricated crack, thereby increasing the crack initiation stress of the shear crack. For instance, in the 30° inclined specimen, the crack initiation stress of the shear crack is 156.25MPa, 100.95MPa, and 92.02MPa when the openings are 0.2mm, 0.4mm, and 2mm, respectively. Based on the stress distribution law around the detected crack, it is evident that the tensile stress concentration area shifts from the middle of the crack to both ends as the crack dip angle increases. At a dip angle greater than 45°, the concentration area is entirely at the end of the crack. The crack opening has little effect on the crack initiation stress and peak strength of the sample when the prefabricated crack angle is greater than 45°. When the prefabricated crack angle is less than 45°, the opening of the crack significantly affects the stress at which the crack initiates and the peak strength of the sample. To describe the degree of closure of prefabricated cracks at different loading stages, we propose a transition model of 'fixed beam → fixed end and hinged end'. Our study reveals the crack propagation law and mechanical mechanism of prefabricated crack specimens with a certain opening under compressive load.
Deep Learning for Intrusion Detection in IoT Networks
Authors: Selem Mehdi , Jemili Farah , Korbaa Ouajdi 
Published: May 2024

Abstract

The rapid proliferation of Internet of Things (IoT) devices has transformed our daily lives, introducing innovations like smart homes, wearables, and advanced industrial automation. While these interconnected systems offer convenience and efficiency, they also present significant security challenges. With the expansion of the IoT network comes an increased risk of malicious attacks, making safeguarding these networks a pressing concern. Intrusion detection serves as a crucial defense mechanism, detecting abnormal activities and triggering appropriate responses. In our study, we harness the power of ensemble learning through a technique known as bagging. By combining the strengths of Deep Neural Networks (DNNs) and Convolutional Neural Networks (CNNs), we aim to capitalize on their unique advantages and enhance the overall capability of intrusion detection systems.
The network and interactive pattern of social adjustment and psychological symptoms in patients with spinal cord injury: A network analysis
Authors: Liu Hui , Ni Yi , Xie Haixia , Xie Yijun , Fan Chenhui , Li Yao , Shen Weinan , Zhai Hua , Jiang Chenghua , Wu Jing , Wu Hengjing 
Published: May 2024

Abstract

Background: The network approach offers a novel framework for conceptualizing depression, anxiety and social adaptability as a causal system, which helps for the understanding of what may be relevant targets for treatment.
Objective：To find the network and interactive pattern of social adjustment and psychological symptoms in patients with spinal cord injury(SCI).
Methods: A cross-sectional parent-reported study was conducted to use an online survey disseminated from February to April 2023. The survey including Medical Coping Modality Questionnaire (MCMQ), Social Spuport Rate Scale (SSRS), Patient Health Questionnaire-9(PHQ-9), and Generalized Anxiety Disorder-7(GAD-7). 251 subjects were completed . Graphical lasso networks and directed acyclic graph (DAG) were used to visualize and analyze the associations between symptom levels corresponding to the four scales.
Results: The average age of patients with SCI was 54.96 years, 61.4% were male. Network analysis results showed that "Concentration" in depression symptoms and "Trouble relaxing" in anxiety symptoms had the highest centrality strength. Anxiety symptom "Nervousness" and depression symptom "Failure" exhibited the highest bridge strength. The result of directed acyclic graph indicated a key triggering role for "Nervousness" symptoms.
Conclusion: "Concentration" in depression symptoms and "Trouble relaxing" in anxiety symptom play a key role in psychological and social adjustment of patients with SCI. It is essential to pay attention to "Nervousness" as it is at the upper end of the symptom response cascade.
Untypical tuneable emission activated by NIR radiation observed in gadolinium borate nanomaterials doped with Yb 3+ , Ho 3+ and Ce 3+ ions
Authors: Szczeszak Agata , Czajka Justyna 
Published: May 2024

Abstract

New luminescent nanoparticles with adjustable emission properties were synthesized via a hydrothermal method to investigate the effect of Ce3+ concentration on the tuneable emission properties of Yb3+/Ho3+ co-doped gadolinium borates. The synthesized samples' comprehensive structural and morphological analysis was conducted utilizing X-ray diffraction and transmission electron microscopy. To obtain highly crystalline nanoparticles GdBO3:Yb3+/Ho3+/Ce3+ characterized by intense and bright emission, they were annealed at 525°C and 925°C. Under the excitation of 972 nm, the tunable yellow to green up-conversion emission was observed, while the Ce3+ concentration increased. This change correlated with a difference in the correlated color temperature from 4345K to 5442K, respectively. The untypical changes observed in the red-to-green (R/G) ratio, displaying a declining trend contrary to conventional expectations, allowed for the proposed mechanism for understanding their UC luminescence properties. The results obtained indicate that the Ce3+ doped GdBO3:Yb3+/Ho3+ nanoparticles demonstrate high application potential for anti-counterfeiting.
Causal association between social support and Osteoarthritis: a two- sample Mendelian randomization study
Authors: Liang Ruiming , Liu Gang , Yuan Lixia 
Published: May 2024

Abstract

Objective: We aimed to study whether social support (SS), including the frequency of friend / family visits (FFV), leisure / social activities (LSA), and ability to talk (AC) are causally associated with Osteoarthritis (OA).
Methods: Data of FFV, LSA, AC and OA in the European population was obtained from the UK Biobank public genome-wide association study (GWAS) summary statistics included in the Mrbase.Three Mendelian randomization (MR) methods, including Inverse variance weighted method(IVW), Weighted median method(WM) and MR-Egger regression were used to evaluate the potential causal effect of FFV, LSA, AC on OA ，and the potential causal effect of OA on FFV, LSA, AC. Heterogeneity was tested using the Cochran's Q test and the I2 statistic. A leave-one-out analysis and test for directional level pleiotropy by MR-Egger regression test served as a sensitivity analysis.
Results: When assessing the causal effect of SS on OA, the IVW method supports causal relationship between FFV and OA risk (beta = -0.02883, SE=0.01293, P=0.02574), and causal relationship between LSA and OA risk (beta =-0.2255, SE=0.067, P =0.0007641) ; The WM method supports a causal relationship between FFV and OA risk (beta = -0.02968, SE=0.01272, P =0.01965), No significant causal relationship between LSA and OA risk was supported by the WM method (beta = -0.1748, SE=0.09266, P=0.05929); The MR-Egger regression did not support a significant causal relationship between FFV, LSA and OA risk. However, the evaluation did not find a causal relationship between AC and OA, nor find a causal relationship between OA and FFV, LSA, AC.The Cochran's Q test and I2 statistics of FFV showed some heterogeneity (MR Egger P=0.003158, I2=0.53452; IWW P = 0.00282, I2=0.53075), and the Cochran's Q test and I2 statistics of LSA showed low heterogeneity (MR Egger P=0.2916, I2=0.100719; IWWP = 0.4652, I2=0.306336). MR-Egger regression analyses of FFV, LSA on OA showed horizontal pleiotropy unlikely to affect the assessment results (FFV intercept =0.0011, P =0.369; LSA intercept =0.0037, P = 0.65).
Conclusion: The results of MR analysis support that FFV and LSA may be causally associated with an decreased risk of OA.FFV and LSA may reduce or delay the development of OA.
An Alternate Approach involving Trans-alveolar Transplantation in a Case Involving Impacted Canines and Retained Deciduous Teeth.
Authors: Thaller Christian , Nolte Dirk , Ludwig Björn , Venugopal Adith 
Published: May 2024

Abstract

Trans-alveolar transplantation, though rarely employed, stands as a viable adjunctive treatment for individuals grappling with severely impacted or ectopic teeth, particularly when executed by a collaborative, multidisciplinary team. In the case of a 25-year-old woman facing a complex palatal impaction involving both maxillary canines alongside aplasia of both lateral incisors, traditional orthodontic traction posed risks of complications. To navigate this challenge successfully, a blend of deciduous and permanent tooth transplantation coupled with fixed orthodontics was employed. Over a meticulous two-year monitoring period, no indications of root resorption or ankylosis surfaced on the transplanted canines, affirming the efficacy of the surgical intervention.
Successful Treatment of Juvenile Polyposis of Infancy With Sirolimus: a case report
Authors: Xiao Pei , Zhang Ting , Wang Yizhong , Xiao Yongmei , Li Xiaolu , Wang Ruixue , Song Ting 
Published: May 2024

Abstract

Background: Infantile juvenile polyposis syndrome (IJPS) is a rare and aggressive form of juvenile polyposis syndrome (JPS) typically diagnosed in the first year of life. It often carries a poor prognosis due to chronic gastrointestinal bleeding, protein losing enteropathy, malnutrition, and immune deficiency.
Case presentation: We report a case of a girl initially presented with pallor at 7 months of age, which progressed to gastrointestinal bleeding and protein-losing enteropathy. Genetic testing identified a 2.1 Mb deletion on chromosome 10q23.2q23.31 involving the phosphatase and tensin homolog (PTEN) and bone morphogenetic protein receptor type IA (BMPR1A) genes. Treatment with sirolimus initiated at 10 months of age led to a reduction in the need for blood and albumin infusions, improved patient growth, and quality of life. While the frequency of endoscopic evaluations decreased with sirolimus, regular endoscopic polypectomy every 5 months remained necessary. However, discontinuation of sirolimus resulted in polyp recurrence after 2 months due to pneumonia.
Conclusion: This case highlights sirolimus treatment can alleviate many complications of IJPS, it does not eliminate the need for aggressive polypectomy.
Metformin regulates LKB1/AMPK/HIF-1α pathways in early stress response to promote angiogenesis and reduce brain damage occasioned by cerebral ischemia
Authors: Chen Hongguang , Yuan Yuting , Zhang Yue , Liu Xiufen , Chen Qingjie , Liu Chao , Yao Qing 
Published: May 2024

Abstract

Purpose
As a difficult-to-treat neurological condition, cerebral ischemia is currently limited to treatments such as intravenous recombinant tissue plasminogen activator thrombolysis and thrombectomy. Metformin, a potent antidiabetic drug, has been reported to have an independent function in enhancing the prognosis of stroke patients, in addition to its glucose-lowering effects. However, the mechanism of metformin in this context remains unclear.
Methods
In vivo, a middle cerebral artery occlusion model was established to measure the infarct area by TTC staining after administering a low dose of 10.5mg/mL metformin, and blood flow in the rat’s cerebral cortex was measured using a Laser Doppler imaging assay. In vitro, the study established human umbilical vein endothelial cells treated with cobalt chloride. Immunofluorescence, immunohistochemistry, and Western blot experiments were performed to observe the expression of angiogenic factors, tight junction proteins, and apoptotic factors. A TUNEL assay was utilized to appraise apoptosis production. A tube formation assay and scratch assay were conducted to determine the endothelial neovascularization status.
Results
Animal experiments have revealed that the administration of the AMPK activator metformin significantly reduced the infarct area, promoted the expression of angiogenic factors, and maintained the stability of tight junction proteins in endothelial cells. Moreover, metformin affects the expression of apoptosis protein in brain tissues through the HIF-1α pathway and reduces the apoptosis of brain cells. In vitro, the LKB1/AMPK signaling pathway is activated after hypoxic stimulation, attaining its peak within the early stages of hypoxia (1–12 hours) and gradually weakening thereafter. The administration of AMPK pharmacological agonists (between 36 and 48 hours) can enhance AMPK activity, which can lead to the expression of angiogenic factors, maintain the stability of tight-junction proteins in endothelial cells, and facilitate endothelial cell migration and vascular structure formation. Conversely, the AMPK inhibitors exert the opposite effects.
Conclusion
The early activation of the LKB1/AMPK/HIF-1α signaling pathway by metformin in cerebral ischemia contributes to angiogenesis, promotes tissue repair in the injured area, and enhances neurologically functional symptoms.
Glutathione modified silicon-doped carbon quantum dots as a sensitive fluorescent probe for ClO - detection
Authors: Ma Xue , Hu Qingqing , Yuan Jingxue , Feng Yao , Cheng Zhengjun 
Published: May 2024

Abstract

A selective and agile fluorescence method for hypochlorite (ClO−) was designed using glutathione (GSH) modified silicon-doped carbon quantum dots (GSH@Si-CDs). Then a dual emission ratio fluorescence probe (RF-probe) was obtained based on carbodiimide-activated coupling reaction between GSH and Si-CDs. i.e., when the excitation wavelength was kept at 360 nm, the GSH@Si-CDs exhibited strong blue and weak yellow fluorescence at 430 and 580 nm. Meanwhile, the fluorescence of GSH@Si-CDs could be selectively quenched at 430 nm and enhanced at 580 nm in the presence of ClO−, and corresponding limit of detection (LOD) and linear range were measured to be 0.35 µM and 1.0-33.3 µM. Moreover, the RF-probe with good accuracy was successfully applied to the assay of ClO− in real samples.
Higgs Decay and CP Violation Phase in the CPV TNMSSM
Authors: Zhu Ning-Yu , Chen Hai-Xiang , Hu Huai-Cong 
Published: May 2024

Abstract

In this study, we calculate the Higgs mass matrix and explore the limitations of the minimumconditions of the scalar potential on parameter degrees of freedom in the CP violation TNMSSM.We discuss the contributions of some parameters to Higgs mass, and their impact on the strength ofHiggs decay signals in different decay channels h →γγ, h →VV (V = W, Z) and h →ff (f = b, c). Usage: Secondary publications and information retrieval purposes.