bluuebunny/crossref_metadata_2025_split huggingface dataset:
A Dibasic Amino Acid Pair Conserved in the Activation Loop Directs Plasma Membrane Localization and Is Necessary for Activity of Plant Type I/II Phosphatidylinositol Phosphate Kinase
Authors: Mikami Koji , Saavedra Laura , Hiwatashi Yuji , Uji Toshiki , Hasebe Mitsuyasu , Sommarin Marianne 
Published: April 2010

Abstract
Phosphatidylinositol phosphate kinase (PIPK) is an enzyme involved in the regulation of cellular levels of phosphoinositides involved in various physiological processes, such as cytoskeletal organization, ion channel activation, and vesicle trafficking. In animals, research has focused on the modes of activation and function of PIPKs, providing an understanding of the importance of plasma membrane localization. However, it still remains unclear how this issue is regulated in plant PIPKs. Here, we demonstrate that the carboxyl-terminal catalytic domain, which contains the activation loop, is sufficient for plasma membrane localization of PpPIPK1, a type I/II B PIPK from the moss Physcomitrella patens. The importance of the carboxyl-terminal catalytic domain for plasma membrane localization was confirmed with Arabidopsis (Arabidopsis thaliana) AtPIP5K1. Our findings, in which substitution of a conserved dibasic amino acid pair in the activation loop of PpPIPK1 completely prevented plasma membrane targeting and abolished enzymatic activity, demonstrate its critical role in these processes. Placing our results in the context of studies of eukaryotic PIPKs led us to conclude that the function of the dibasic amino acid pair in the activation loop in type I/II PIPKs is plant specific.
Neutropenia dynamics in a case of T‐LGL lymphoproliferation illustrate rapid turnover of granulocyte progenitors
Authors: Wolfrom C. M. , Lévy V. , Deschatrette J. 
Published: April 2010

AbstractObjectives:  To elucidate the natural history of T‐cell large granular lymphocyte (T‐LGL) lymphoproliferation, we followed changes in associated fluctuating neutropenia for 3 years in an untreated patient presenting with the disease.Materials and methods:  We report a nonlinear mathematical analysis of irregular neutrophil fluctuation, using iterative data maps, to detect long‐term regulation of the neutrophil population.Results:  This geometric analysis indicated that variations of this sequence of neutrophil counts followed bounded deterministic dynamics around a fixed low level equilibrium, a situation similar to that previously observed for cultured mouse early bone marrow progenitor cells.Conclusion:  These findings illustrate how the deleterious effect of T‐LGL on neutrophils is balanced, over periods of years, by pulses of compensatory neutrophil production, potentially accounting for the commonly observed prolonged indolent course of the disease.
Impact of cross‐reactive carbohydrate determinants on wood dust sensitization
Authors: Kespohl S. , Schlünssen V. , Jacobsen G. , Schaumburg I. , Maryska S. , Meurer U. , Brüning T. , Sigsgaard T. , Raulf‐Heimsoth M. 
Published: April 2010

SummaryBackground
Occupational wood dust exposure can induce allergy and may be one cause of respiratory health problems among woodworkers.Objective
The objective was to determine the prevalence and quantitative level of specific immunoglobulin E (sIgE) to beech and pine wood in exposed workers. Wood sensitization was specified with regard to cross‐reactivity and was correlated to the reported symptoms.Methods
Danish workers (n=701) were investigated for sIgE to beech and pine. Wood samples from workplaces were analysed and coupled to ImmunoCAPs. Workers sensitized to wood were tested for cross‐reactive carbohydrate determinants (CCDs) and environmental allergens. IgE binding was specified for glycogenic vs. proteinogenic epitopes by inhibition tests.Results
The prevalence of wood sensitization among all workers was 3.7%. There was no association between sensitization prevalence or sIgE concentrations and self‐reported allergic symptoms. Beech‐ and pine‐sensitized workers showed a high prevalence of CCD sensitization (73%). However, workers with a single sensitization to wood had no sIgE to CCDs. Specifying IgE epitopes demonstrated that sera of workers reporting allergic symptoms recognized proteinogenic IgE‐epitopes on wood allergens, whereas workers without allergic symptoms had primarily sIgE‐epitopes to glycogenic structures. Although 96% of the wood‐sensitized workers were atopic, no significant correlation was found between wood sensitization and sIgE to beech and birch pollen, but an association was found between sIgE against CCDs and pine pollen.Conclusion
Sensitization prevalence to beech and pine wood measured by tailored ImmunoCAPs was not correlated to allergic symptoms. We recommend the application of CCD tools to assess the relevance of individual wood sensitization. Cite this as: S. Kespohl, V. Schlünssen, G. Jacobsen, I. Schaumburg, S. Maryska, U. Meurer, T. Brüning, T. Sigsgaard and M. Raulf‐Heimsoth, Clinical & Experimental Allergy, 2010 (40) 1099–1106.
Beclomethasone dipropionate blunts allergen‐induced early increase in urinary LTE4
Authors: Bartoli Maria Laura , Dente Federico Lorenzo , Bancalari Lorenza , Bacci Elena , Cianchetti Silvana , Di Franco Antonella , Vagaggini Barbara , Paggiaro Pier Luigi 
Published: April 2010

Eur J Clin Invest 2010; 40 (6): 566–569AbstractBackground  The inhibitory effect of corticosteroids (CS) on the secretions of cysteinyl‐leukotrienes (Cys‐LTs) in asthma is controversial. The aim of this study was to evaluate the effect of CS on allergen‐induced increase in urinary leukotriene E4 (uLTE4) during early (EAR) and late (LAR) asthmatic responses in mild untreated asthmatics.Material and methods  Nine subjects with mild untreated allergic asthma performed two allergen challenges, after 1‐week treatment with beclomethasone dipropionate (BDP, 500 μg b.i.d) or placebo. Forced Expiratory Volume in one second 1 (FEV1) was monitored to assess EAR and LAR, and uLTE4 was measured before and during EAR and LAR.Results  After placebo, uLTE4 increased significantly during EAR, but not during and after LAR, in comparison with baseline values. Beclomethasone dipropionate induced a significant attenuation of the uLTE4 increase during EAR, in comparison with placebo, in association with a good protection of LAR (P = 0·002) and a mild protection of EAR (P = 0·07).Conclusions  Beclomethasone dipropionate blunts the early increase in uLTE4 excretion due to allergen challenge, in association with a significant effect on the severity of LAR. These data support the hypothesis that inhaled CS may inhibit the allergen‐induced release of cys‐LTs in asthma.
Endocrine tumours of the pancreas
Authors: Verbeke Caroline S 
Published: April 2010

Verbeke C S  (2010) Histopathology 56, 669–682 Endocrine tumours of the pancreasHistopathology reporting of pancreatic endocrine neoplasms is complex. The tumours can exhibit a variety of morphological appearances, which often require careful differential diagnostic consideration. Prediction of tumour behaviour and clinical outcome is based on the World Health Organization classification and TNM staging and grading system, which share some criteria and premises, but differ significantly in others. Clinicopathological correlation through discussion at multidisciplinary team meetings is of paramount importance. In this review special emphasis is given to the items of information that can and should be provided by the pathologist to allow optimal patient management. The review further discusses areas of current controversy and uncertainty, of which pathologists participating in multidisciplinary discussions should be aware.
HER2 assessment on core biopsy specimens using monoclonal antibody CB11 accurately determines HER2 status in breast carcinoma
Authors: Purdie Colin A , Jordan Lee B , McCullough Jean B , Edwards Sharon L , Cunningham Joan , Walsh Miriam , Grant Andrew , Pratt Norman , Thompson Alastair M 
Published: April 2010

Purdie C A, Jordan L B, McCullough J B, Edwards S L, Cunningham J, Walsh M, Grant A, Pratt N & Thompson A M (2010) Histopathology 56, 702–707 HER2 assessment on core biopsy specimens using monoclonal antibody CB11 accurately determines HER2 status in breast carcinomaAims:  HER2 status is a prognostic factor in breast carcinoma and predicts response to trastuzumab therapy. Trastuzumab is effective in the neoadjuvant, adjuvant and advanced disease settings. Knowledge of HER2 status early in the diagnostic and therapeutic process is vital for treatment planning. HER2 analysis is usually carried out by immunohistochemistry or fluorescence in situ hybridization (FISH). The aim of this study was to establish whether HER2 immunohistochemistry using monoclonal antibody CB11 and carried out on the initial diagnostic core biopsy specimen accurately predicts HER2 amplification status.Methods and results: This is the largest study to date in which HER2 protein expression has been assessed by CB11 immunohistochemistry on the diagnostic core biopsy specimen and correlated with the result of FISH. Using FISH as the definitive HER2 status, we studied 568 invasive breast cancers using CB11 immunohistochemistry on core biopsy. This analysis had a sensitivity of 99.4%, specificity of 93.9%, false‐positive frequency of 3.9% and false‐negative frequency of 1.1%. These data are as good as those obtained from analysing resection specimens alone in UK national reference centres.Conclusions: CB11 immunohistochemistry accurately predicts HER2 amplification status and can be reliably carried out on core biopsy specimens of breast carcinoma.
Stromal CD10 expression and relationship to the E‐cadherin/β‐catenin complex in breast carcinoma
Authors: Kim Hyun‐Soo , Kim Gou Young , Kim Youn Wha , Park Yong‐Koo , Song Jeong‐Yoon , Lim Sung‐Jig 
Published: April 2010

Kim H‐S, Kim G Y, Kim Y W, Park Y‐K, Song J‐Y & Lim S‐J (2010) Histopathology 56, 708–719 Stromal CD10 expression and relationship to the E‐cadherin/β‐catenin complex in breast carcinomaAims:  Previous investigations have indicated that stromal CD10 expression, and altered levels of both E‐cadherin and β‐catenin, are associated with the biological aggressiveness of human carcinoma. The aim was to evaluate stromal CD10 expression and the association of stromal CD10 with E‐cadherin and β‐catenin in breast carcinoma.Methods and results:  The expression of CD10, E‐cadherin and β‐catenin was immunohistochemically analysed in tissue microarrays containing 104 cases of invasive ductal carcinoma (IDC) and 10 cases of ductal carcinoma in situ (DCIS). Stromal CD10 was detected in 49.5% (50/101) of the IDC. No immunoreactivity was identified in the stromal cells of normal breast, DCIS or intraductal components of IDC. Accumulation of the cytoplasmic β‐catenin was found in 87.0% (87/100) of the IDC. Stromal CD10 expression in IDC was significantly correlated with tumour size (P = 0.027), stage (P < 0.001) and histological grade (P = 0.006), the presence of nodal (P = 0.048) and distant (P = 0.015) metastases, oestrogen receptor‐negative status (P = 0.016), cytoplasmic β‐catenin accumulation (P = 0.031) and lower overall survival rate (P = 0.041).Conclusions:  Stromal CD10 expression in IDC may constitute an important prognostic marker. Stromal CD10 expression with associated aggressive features might be related to aberrant β‐catenin expression.
Expression of HSP90 in gastrointestinal stromal tumours and mesenchymal tumours
Authors: Kang Gu Hyun , Lee Eui Jin , Jang Ki Taek , Kim Kyoung‐Mee , Park Cheol Keun , Lee Choong‐Sik , Kang Dae Young , Lee Sug Hyung , Sohn Tae Sung , Kim Sung 
Published: April 2010

Kang G H, Lee E J, Jang K T, Kim K‐M, Park C K, Lee C‐S, Kang D Y, Lee S H, Sohn T S & Kim S (2010) Histopathology 56, 694–701 Expression of HSP90 in gastrointestinal stromal tumours and mesenchymal tumoursAims:  Heat shock proteins (HSP) are up‐regulated under conditions of increased stress, including cancer. Recently, HSP90 has been shown to be crucial to the expression and activation of the KIT oncoprotein. The aim was to explore the role of HSP90 expression as a prognostic marker and therapeutic target in gastrointestinal stromal tumours (GISTs) and other mesenchymal tumours.Methods and results:  The expression of HSP90 was evaluated by immunohistochemistry in 92 GISTs, 47 mesenteric fibromatoses, six schwannomas, leiomyomas, melanomas, malignant peripheral nerve sheath tumours and leiomyosarcomas. Western blotting was performed in 22 selected cases. HSP90 overexpression was found in 33.7% of GISTs and was correlated with non‐gastric location, mixed histological subtype, high mitotic index, high risk grades, and specific mutation genotypes. In mesenchymal tumours, HSP90 overexpression was found in 66.7% of malignant peripheral nerve sheath tumours, 83.3% of leiomyosarcomas, and 100% of melanomas. HSP90 expression by Western blotting correlated with the results of immunohistochemistry. The Cox proportional hazards model showed that HSP90 expression is an independent predictor of recurrence in GISTs (P = 0.003).Conclusions:  Overexpression of HSP90 is predictive of adverse behaviour in GISTs and may provide a therapeutic solution to the challenge of imatinib‐resistant GISTs and other mesenchymal sarcomas.
ILK expression in human basal cell carcinoma correlates with epithelial–mesenchymal transition markers and tumour invasion
Authors: Papanikolaou Sofia , Bravou Vasiliki , Gyftopoulos Konstantinos , Nakas Dimitrios , Repanti Maria , Papadaki Helen 
Published: April 2010

Papanikolaou S, Bravou V, Gyftopoulos K, Nakas D, Repanti M & Papadaki H (2010) Histopathology 56, 799–809 ILK expression in human basal cell carcinoma correlates with epithelial–mesenchymal transition markers and tumour invasionAims:  Epithelial–mesenchymal transition (EMT) has been known to play a significant role in tumour progression. Integrin‐linked kinase (ILK) has been recently added to the growing list of EMT regulators that control some aspect of carcinogenesis. The aim was to study ILK expression and its relevance to EMT markers in human basal cell carcinoma (BCC).Methods and results:  Paraffin‐embedded tissue sections from 100 human BCC cases were processed by immunohistochemistry for the expression of ILK, E‐cadherin, Snail, β‐catenin and alpha‐smooth muscle actin (α‐SMA). ILK overexpression was observed in 100% of cases and strongly correlated with tumour invasion and infiltrative BCC. Loss of membranous E‐cadherin was found in 71% of cases while nuclear immunoreactivity for E‐cadherin was also observed in 90% of the tumours. Snail, nuclear β‐catenin and α‐SMA expression was detected in 100%, 99% and 97% of tumours, respectively. Aberrant expression of E‐cadherin, nuclear β‐catenin and α‐SMA correlated with BCC tumour invasion. Interestingly, there was a significant correlation between ILK expression and all the EMT markers examined.Conclusions:  ILK overexpression in BCC is implicated in tumour progression probably through the induction of an EMT‐related molecular profile. Nuclear localization of E‐cadherin in BCC is also associated with aggressive tumour features.
Low plasma adiponectin concentration is associated with myocardial infarction in young individuals
Authors: Persson J. , Lindberg K. , Gustafsson T. P. , Eriksson P. , Paulsson‐Berne G. , Lundman P. 
Published: April 2010

Abstract.  Persson J, Lindberg K, Gustafsson TP, Eriksson P, Paulsson‐Berne G, Lundman P. (Danderyd University Hospital; Karolinska Institutet, Novum; Karolinska University Hospital, Karolinska Institutet; Atherosclerosis Research Unit; Karolinska Institutet, Stockholm, Sweden). Low plasma adiponectin concentration is associated with myocardial infarction in young individuals. J Intern Med 2010; 268: 194–205.Objective.  The importance of adiponectin in coronary heart disease remains to be elucidated. Therefore, the associations between plasma adiponectin levels and i) myocardial infarction and ii) genetic variation within the adiponectin gene were investigated.Methods.  The study included young survivors (age <60 years) of a first myocardial infarction and gender‐ and age‐matched controls (244 pairs). Adiponectin concentrations were analysed by radioimmunoassay. Two polymorphisms, rs266729 and rs1501299, of the adiponectin gene ADIPOQ were genotyped.Results.  Adiponectin levels were inversely associated with myocardial infarction [odds ratio (OR) 9.3, 95% confidence interval (CI) 4.7–18.2, for the lowest quartile compared to the highest quartile]. This persisted after adjustment for history of hypertension, HDL cholesterol, smoking and body mass index (BMI) (OR 3.1, 95% CI 1.3–7.6). The rs266729 polymorphism was associated with adiponectin levels. Plasma adiponectin concentrations were lower in individuals with the rare G/G genotype [median 4.3 mg mL−1, interquartile range (IQR) 2.8–6.2] compared to the C/G (median 5.8 mg mL−1, IQR 3.9–8.0; P = 0.035) and C/C genotypes (median 5.5 mg mL−1, IQR 4.0–7.5; P = 0.083).Conclusion.  Low plasma adiponectin concentrations are associated with myocardial infarction in individuals below the age of 60, and this remains significant after adjustment for history of hypertension, HDL cholesterol, smoking and BMI. In addition, adiponectin levels differ according to rs266729 genotype.
Gallstone disease in Swedish twins: risk is associated with ABCG8 D19H genotype
Authors: Katsika D. , Magnusson P. , Krawczyk M. , Grünhage F. , Lichtenstein P. , Einarsson C. , Lammert F. , Marschall H.‐U. 
Published: April 2010

Abstract.  Katsika D, Magnusson P, Krawczyk M, Grünhage F, Lichtenstein P, Einarsson C, Lammert F, Marschall H‐U (Karolinska University Hospital Huddinge, Karolinska Institutet, Stockholm, Sweden; Karolinska Institutet, Stockholm, Sweden; and Saarland University Hospital, Homburg, Germany). Gallstone disease in Swedish twins: risk is associated with ABCG8 D19H genotype. J Intern Med 2010; 268: 279–285.Objective.  Recently, variants of the hepatocanalicular cholesterol hemitransporters ABCG5/8 were linked to gallstone disease; ABCG8 D19H in Caucasians and ABCG5 Q604E in Chinese. We investigated these polymorphisms in Swedish twins by merging the Swedish Twin Registry with the Hospital Discharge and Causes of Death Registries for gallstone disease‐related diagnoses.Design.  All monozygotic (MZ) twins with gallstone disease alive in the Stockholm area were invited to participate. Gallstone disease was defined by entry in all above mentioned registries, questionnaire or abdominal ultrasound.Subjects.  ABCG5 Q604E and ABCG8 D19H genotyping was performed in 24 unique MZ and eight dizygotic (DZ) twins from concordant pairs. Screening of the TwinGene database for gallstone disease resulted in an additional 20 concordant MZ and 54 twins from concordant DZ pairs. We included 109 concordantly stone‐free MZ and 126 stone‐free independent DZ twins as controls.Results.  Amongst the 341 twins, 20.8% carried at least one D19H allele as compared to 9.4% of stone‐free controls. The association analysis showed that D19H positivity significantly increased the risk of gallstone disease [odds ratio (OR), 2.54; 95% confidence interval (CI), 1.33–4.82; P = 0.004]. We also found a trend for a positive association between gallstone disease and the Q604E variant (OR, 1.47; 95% CI, 1.00–2.16; P = 0.052).Conclusion.  Twins carrying a heterozygous or homozygous ABCG8 D19H genotype have a significantly increased risk of gallstone disease. Our study confirms the ABCG8 D19H genotype as a major risk factor for gallstone disease.
Co‐occurring increases of calcium and organellar reactive oxygen species determine differential activation of antioxidant and defense enzymes in Ulva compressa (Chlorophyta) exposed to copper excess
Authors: GONZALEZ ALBERTO , VERA JEANNETTE , CASTRO JORGE , DENNETT GERALDINE , MELLADO MACARENA , MORALES BERNARDO , CORREA JUAN A. , MOENNE ALEJANDRA 
Published: April 2010

ABSTRACTIn order to analyse copper‐induced calcium release and (reactive oxygen species) ROS accumulation and their role in antioxidant and defense enzymes activation, the marine alga Ulva compressa was exposed to 10 µM copper for 7 d. The level of calcium, extracellular hydrogen peroxide (eHP), intracellular hydrogen peroxide (iHP) and superoxide anions (SA) as well as the activities of ascorbate peroxidase (AP), glutathione reductase (GR), glutathione‐S‐transferase (GST), phenylalanine ammonia lyase (PAL) and lipoxygenase (LOX) were determined. Calcium release showed a triphasic pattern with peaks at 2, 3 and 12 h. The second peak was coincident with increases in eHP and iHP and the third peak with the second increase of iHP. A delayed wave of SA occurred after day 3 and was not accompanied by calcium release. The accumulation of iHP and SA was mainly inhibited by organellar electron transport chains inhibitors (OETCI), whereas calcium release was inhibited by ryanodine. AP activation ceased almost completely after the use of OETCI. On the other hand, GR and GST activities were partially inhibited, whereas defense enzymes were not inhibited. In contrast, PAL and LOX were inhibited by ryanodine, whereas AP was not inhibited. Thus, copper stress induces calcium release and organellar ROS accumulation that determine the differential activation of antioxidant and defense enzymes.
Infrared and fundus autofluorescence imaging in eyes with optic disc pit maculopathy
Authors: Hiraoka Tomoyuki , Inoue Makoto , Ninomiya Yuko , Hirakata Akito 
Published: April 2010

AbstractBackground:  To characterize the infrared (IR) and fundus autofluorescence (FAF) images of eyes with optic disc pit maculopathy.Methods:  A retrospective non‐comparative study of three patients with optic disc pit maculopathy who were followed by optical coherence tomography (OCT) and retinal angiography (Heidelberg retina angiograph 2) before and after vitreous surgery without laser photocoagulation.Results:  The areas of serous retinal detachment and inner retinal schisis were dark in the IR and FAF images preoperatively; and they changed to brighter areas following reattachment of the retina. There was an increase in the granular hyperfluorescence in the FAF images accompanied by an increase in the number of subretinal precipitates. OCT showed a thickening of the photoreceptor outer segments. The outer retinal layer defect initially appeared bright in the IR images and hypofluorescent or hyperfluorescent in the FAF images. The outer retinal layer defect became larger with a reduction of the outer retinal schisis and increased retinal detachment, but then became smaller with the resolution of the retinal detachment. All cases had a resolution or reduction of the retinal detachment and retinal schisis after the vitrectomy.Conclusion:  The IR and FAF images in eyes with optic disc pit maculopathy reflect the changes in the inner and outer retinal layers corresponding with the stage of recovery of the disease.