bluuebunny/crossref_metadata_2025_split huggingface dataset:
HspB8 mutation causing hereditary distal motor neuropathy impairs lysosomal delivery of autophagosomes
Authors: Kwok Alice S. , Phadwal Kanchan , Turner Bradley J. , Oliver Peter L. , Raw Annie , Simon Anna Katharina , Talbot Kevin , Agashe Vishwas R. 
Published: October 2011

J. Neurochem. (2011) 10.1111/j.1471‐4159.2011.07521.xAbstractHspB8, a small heat‐shock protein implicated in autophagy, is mutated in patients with distal hereditary motor neuropathy type II (dHMNII). Autophagy is essential for maintaining protein homeostasis in the central nervous system, but its role has not been investigated in peripheral motor neurons. We used a novel, multispectral‐imaging flow cytometry assay to measure autophagy in cells. This assay revealed that over‐expression of wild‐type HspB8 in motor neuron‐like NSC34 cells led to an increased co‐localisation of autophagosomes with the lysosomes. By contrast, over‐expression of mutant HspB8 resulted in autophagosomes that co‐localised with protein aggregates but failed to co‐localise with the lysosomes. A similar impairment of autophagy could also be demonstrated in peripheral blood mononuclear cells from two dHMNII patients with the HspB8K141E mutation. We conclude that defects in HspB8‐mediated autophagy are likely to contribute to dHMNII pathology and their detection in peripheral blood mononuclear cells could be a useful, accessible biomarker for the disease.
Increased dosage of tumor suppressors limits the tumorigenicity of iPS cells without affecting their pluripotency
Authors: Menendez Sergio , Camus Suzanne , Herreria Aida , Paramonov Ida , Morera Laura B. , Collado Manuel , Pekarik Vlad , Maceda Iago , Edel Michael , Consiglio Antonella , Sanchez Adriana , Li Han , Serrano Manuel , Belmonte Juan C. I. 
Published: October 2011

SummaryEmbryonic stem (ES) cells and induced pluripotent stem (iPS) cells represent a promising therapeutic tool for many diseases, including aged tissues and organs at high risk of failure. However, the intrinsic self‐renewal and pluripotency of ES and iPS cells make them tumorigenic, and hence, the risk of tumor development hinders their clinical application. Here, we present a novel approach to limit their tumorigenicity and increase their safety through increased copy number of tumor suppressors. iPS containing an extra copy of the p53 or Ink4a/ARF locus show normal pluripotency, as determined by in vitro and in vivo differentiation assays. Yet, while retaining full pluripotency, they also possess an improved engagement of the p53 pathway during teratocarcinoma formation, which leads to a reduced tumorigenic potential in various in vitro and in vivo assays. Furthermore, they show an improved response to anticancer drugs, which could aid in their elimination in case tumors arise with no adverse effects on cell function or aging. Our system provides a model for studying tumor suppressor pathways during reprogramming, differentiation, and cell therapy applications. This offers an improved understanding of the pathways involved in tumor growth from engrafted pluripotent stem cells, which could facilitate the use of ES and iPS cells in regenerative medicine.
Everolimus‐induced loss of wound strength can be prevented by a short postoperative delay in its administration
Authors: Willems Martine C. M. , Hendriks Thijs , de Man Ben M. , Lomme Roger M. L. M. , van der Vliet J. Adam 
Published: October 2011

AbstractThe use of mammalian target of rapamycin inhibitors coincides with an increased incidence of surgical complications. In previous experiments, serious negative effects of postoperative everolimus on anastomotic strength were found. This study aims to investigate if delayed drug administration can prevent loss of wound strength. Ten groups of Wistar rats each received daily oral doses of 1.0 or 2.0 mg/kg everolimus, starting the day of anastomotic construction in both ileum and colon, or 1, 2, 3, or 4 days later. The 11th group received saline. Seven days later, wound strength in anastomoses and in the abdominal wall and wound hydroxyproline levels were measured. Mean wound strength was significantly and dose‐dependently reduced if everolimus was started on the day of operation. In ileum and colon, strength was not affected if drug administration was delayed until the third or second day, respectively. In abdominal fascia, this was the case only if everolimus was withheld until day 4. In general, changes in wound hydroxyproline content showed similarities to changes in wound strength. Thus, delaying administration of everolimus for 2–4 days after operation can prevent a serious loss of wound strength, both in the intestine and in the abdominal fascia.
Collagen matrix‐bound clotting factors (CMBCF) promote healing‐associated events independent of factor XIII in an in vitro model
Authors: Pohle Thorsten , Becker Jan C. , Lügering Andreas 
Published: October 2011

AbstractWe have previously explored in vitro as well as in vivo models of the biological effects of liquid fibrin glue (FG) containing factor XIII. The fixed combination of a collagen matrix and coagulation factors I and IIa (TachoSil®, Nycomed, Linz, Austria) is void of factor XIII. We aimed to determine whether (1) this preparation exerts similar effects to liquid FG on cells in an in vitro system, or (2) this effect is modulated by factor XIII. In an in vitro model, the effect of the fixed combination of collagen matrix and coagulation factors I and IIa (collagen matrix‐bound clotting factor [CMBCF]) on the expression and secretion of growth factors (vascular endothelial growth factor, platelet‐derived growth factor, fibroblast growth factor‐2) by gastric epithelial (AGS) and mesenchymal cells (fibroblasts), as well as their proliferative response (WST‐test), was compared in the presence and absence of factor XIII. The use of CMBCF compared with collagen type I matrix resulted in an increased proliferation rate of fibroblasts; there was an increased secretion of fibroblast growth factor‐2. Gastric epithelial cells secreted more vascular endothelial growth factor and platelet‐derived growth factor into the culture supernatant in the presence of CMBCF. All responses remained unaltered by the addition of factor XIII in different concentrations. In conclusion, CMBCF exerted effects similar to liquid FG in an in vitro model of healing. The addition of factor XIII did not alter the response of mesenchymal or epithelial cells, with respect to proliferation and growth factor secretion.
Metamorphic evolution of lawsonite eclogites from the southern Motagua fault zone, Guatemala: insights from phase equilibria and Raman spectroscopy
Authors: ENDO S. , WALLIS S. R. , TSUBOI M. , TORRES DE LEÓN R. , SOLARI L. A. 
Published: October 2011

AbstractLawsonite eclogite (metabasalt and metadolerite) and associated metasedimentary rocks in a serpentinite mélange from an area just south of the Motagua fault zone (SMFZ), Guatemala, represent excellent natural records of the forearc slab–mantle interface. Pseudosection modelling of pristine lawsonite eclogite reproduces the observed predominant mineral assemblages, and garnet compositional isopleths intersect within the phase fields, yielding a progradeP–Tpath that evolves from 20 kbar, 470 °C (M1) to 25 kbar, 520 °C (M2). The dominant penetrative foliation within the eclogite blocks is defined by minerals developed during the prograde evolution, and the associated deformation, therefore, took place during subduction. Thermometry using Raman spectra of carbonaceous material in metasedimentary rocks associated with the SMFZ eclogites gives estimates of peak‐Tof ∼520 °C. Barometry using Raman spectroscopy shows unfractured quartz inclusions in garnet rims retain overpressures of up to ∼10 kbar, implying these inclusions were trapped at conditions just below the quartz/coesite transition, in agreement with the results of phase equilibrium analysis. Additional growth of Ca‐rich garnet indicates initial isothermal decompression to 20 kbar (M3) followed by hydration and substantial cooling to the lawsonite–blueschist facies (M4). Further decompression of the hydrated eclogite blocks to the pumpellyite–actinolite facies (3–5 kbar, 230–250 °C) is associated with dehydration and veining (M5). The presence of eclogite as m‐ to 10 m‐sized blocks in a serpentinite matrix, lack of widespread deformation developed during exhumation and derived progradeP–Tpath associated with substantial dehydration of metabasites within the antigorite stability field suggest that the SMFZ eclogites represent the uppermost part of the forearc slab crust sampled by an ascending serpentinite diapir in an active, moderate‐Tsubduction zone.
Conversion of a Partial Removable Dental Prosthesis from Kennedy Class II to Class III Using a Dental Implant and Semiprecision Attachments
Authors: Liu Ruohong , Kaleinikova Zina , Holloway Julie A. , Campagni Wayne V. 
Published: October 2011

AbstractThis article presents a design to convert a partial removable dental prosthesis (PRDP) from Kennedy class II to class III using a dental implant. Incorporating semiprecision attachments, this design provides desired esthetics, phonetics, and function.
The Importance of Class‐I Antiarrhythmic Drug Test in the Evaluation of Patients with Syncope: Unmasking Brugada Syndrome
Authors: ROOS MARKUS , SARKOZY ANDREA , BRODBECK JEANNETTE , HENKENS STEFAN , CHIERCHIA GIAN‐BATTISTA , ASMUNDIS CARLO DE , CAPULZINI LUCIO , MÜLLER‐BURRI STEPHAN ANDREAS , YAKAZI YOSHI , BRUGADA PEDRO 
Published: October 2011

Syncope in Brugada Syndrome. Introduction: The Brugada syndrome (BrS) can first present with syncope. Class‐I antiarrhythmic drug (AAD) test is used to unmask the diagnostic coved‐type ECG pattern in case it is not spontaneously present. The aim of the study was to analyze patients with BrS presenting with syncope as first manifestation and compare patients with syncope and a spontaneous coved‐type ECG to patients with syncope in whom a class‐I AAD test unmasked the disease.
Methods and Results: Fifty‐eight of 157 probands (36.9%) had syncope as first manifestation of the disease. Twenty‐six patients (44.8%, group A) showed a spontaneous coved‐type ECG diagnostic for BrS at first presentation. In 32 patients (55.2%, group B) without spontaneous coved‐type ECG pattern at first presentation (36% normal ECGs and 19% type‐II ECG pattern), a class‐I AAD test unmasked the disease. Twenty‐one patients of group A and 29 patients of group B underwent implantable cardioverter defibrillator (ICD) implantation. The mean follow up as 9.7 ± 55.7 month. Four patients in group A (15.4%) and 3 patients (9.3%) in group B had appropriate ICD shock delivery due to ventricular fibrillation or ventricular tachycardia (P = NS).
Conclusion: One of 3 patients with BrS presents first with syncope. More than one‐third of these patients have a normal ECG at investigation for syncope and the correct diagnosis would have been missed without a class‐I AAD test. Patients presenting with syncope are at similar risk irrespective of the presence of a spontaneous coved‐type ECG. (J Cardiovasc Electrophysiol, Vol. 23 p. 290‐295, March 2012.)
Long‐Term Safety and Effectiveness of Drug‐Eluting Stents Compared to Bare Metal Stents following Successful PCI in Non‐ST‐Elevation Myocardial Infarction: Findings from the Guthrie Health Off‐Label StenT (GHOST) Registry
Authors: HARJAI KISHORE J. , SATTUR SUDHAKAR , ORSHAW PAMELA , BOURA JUDY 
Published: October 2011

Background:
The long‐term safety and effectiveness of drug‐eluting stents (DES) versus bare metal stents (BMS) in non‐ST‐segment elevation myocardial infarction (NSTEMI) beyond 2 years after percutaneous coronary intervention (PCI) is unknown.
Methods:
We studied 674 NSTEMI patients who underwent successful PCI with DES (n = 323) or BMS (n = 351). The primary study end‐points were time to occurrence of death or nonfatal recurrent myocardial infarction (MI), and stent thrombosis (ST). Secondary end‐points included time to occurrence of target vessel revascularization (TVR) and any major adverse cardiovascular event (MACE, defined as the composite of death, MI, ST, TVR).
Results:
The DES and BMS groups were well matched except that DES patients received dual antiplatelet therapy for a longer duration and had smaller final vessel diameter. In survival analysis, at a mean follow‐up of 1333 ± 659 days after PCI, the DES group had similar incidence of death/myocardial infarction (24% vs. 27%, log rank p = 0.23) and ST (4.0% vs. 2.6%, p = 0.18) as the BMS group. The DES patients had lower incidence of TVR (8.1% vs. 17%, p = 0.0018) but similar MACE (26% vs. 37%, p = 0.31). In multivariable analysis, DES vs. BMS implantation showed no significant impact on death/myocardial infarction [adjusted hazards ratio (HR) 1.0, 95% confidence intervals (CI) 0.7–1.4], ST (HR 1.7; CI 0.7 – 4.0), or MACE (HR 0.8; CI 0.6 – 1.1). However, TVR was lower in the DES group (HR 0.4; CI 0.3 – 0.7).
Conclusion:
In patients presenting with NSTEMI, DES implantation appears to be as safe as BMS implantation at long‐term follow‐up. In addition, DES are effective in reducing TVR compared to BMS. (J Interven Cardiol 2012;25:28–36)
Transfemorally or Transapically Deployed Sapien Edwards Bioprosthesis Is Always Deformed
Authors: ALLAM BACHIR , ZEGDI RACHID , BLANCHARD DIDIER , CHOLLEY BERNARD , FABIANI JEAN‐NOËL , LAFONT ANTOINE 
Published: October 2011

Objective:
To study the impact of femoral compared to apical access on the Sapien‐Edwards (SE) prosthesis deployment and geometry in patients treated with transcatheter aortic valve implantation (TAVI) for aortic stenosis.
Background:
SE prosthesis deformation exists after its deployment through transfemoral (TF ‐ TAVI) approach. However, no study comparing the deformation between TF ‐ TAVI and transapical (TA ‐ TAVI) approaches has yet been published.
Methods:
Forty consecutive patients received TAVI with the SE prosthesis (TF ‐ TAVI n  =  25; TA ‐ TAVI n  =  15). A fluoroscopic analysis of the prosthesis was then performed. The stent frame geometry was assessed during deployment in the profile view, and after implantation in the profile and frontal views.
Results:
Expansion kinetics revealed a triphasic stent deployment with both approaches; the aortic extremity being the first to open. After implantation, on the profile view, the stent shape was never rectangular (therefore never cylindrical) in both groups. It had a biconic shape in most of the patients (76% vs. 93.3% for TF ‐ TAVI and TA ‐ TAVI patients, respectively, P  =  0.224) with a wider aortic extremity relative to the ventricular one. The frontal view analysis showed that circular deployment of the stent was never achieved. A greater leaflet to stent mismatch was noted in TA ‐ TAVI patients, however, the difference was not statistically significant (12% vs. 33.3%, P  =  0.126).
Conclusion:
Fluoroscopically assessed, the geometry of SE prosthesis was never cylindrical after deployment, whatever the access for implantation was. Longitudinal deformation was greater after TF ‐ TAVI whereas leaflet to stent mismatch tended to be more pronounced after TA ‐ TAVI. (J Interven Cardiol 2012;25:53–61)
Serum high‐density lipoprotein cholesterol level associated with the extent of periodontal inflammation in type 1 diabetic subjects
Authors: Passoja Anna , Knuuttila Matti , Hiltunen Liisa , Karttunen Riitta , Niemelä Onni , Raunio Taina , Vainio Olli , Hedberg Pirjo , Tervonen Tellervo 
Published: October 2011

AbstractAimHigh‐density lipoprotein (HDL) cholesterol is known for its anti‐inflammatory and antioxidant activities in protection against cardiovascular diseases. We investigated whether a protective association also exists between serum HDL and periodontal inflammation in type 1 diabetic subjects (T1DM).MethodsPlaque and periodontal inflammation (bleeding and PD ≥ 4 mm) were examined in 80 subjects with T1DM. The serum levels of glycosylated haemoglobin (HbA1c, %) and HDL (mmol/l) were determined. Adjusted associations between inflammation and serum HDL were analysed using linear regression analysis. To study the linearity of the association, the subjects were categorized into HDL tertiles (I‐III).ResultsA statistically significant negative association was observed between serum HDL level and the extent of bleeding and PD ≥ 4 mm. Subjects in HDL tertiles II and III (high HDL) presented significantly fewer inflamed sites when compared with the subjects in tertile I (low HDL), whereas no significant difference in the number of inflamed sites was observed between tertiles II and III.ConclusionsBased on the finding of a negative association between serum HDL and periodontal inflammation, HDL may be considered a marker of susceptibility to periodontal inflammation. A longitudinal study is needed to verify possible causal relationship between serum HDL and inflammation.
Hydrogen‐rich water attenuates experimental periodontitis in a rat model
Authors: Kasuyama Kenta , Tomofuji Takaaki , Ekuni Daisuke , Tamaki Naofumi , Azuma Tetsuji , Irie Koichiro , Endo Yasumasa , Morita Manabu 
Published: October 2011

AbstractAimReactive oxygen species (ROS) contribute to the development of periodontitis. As molecular hydrogen can act as a scavenger of ROS, we examined the effects of treatment with hydrogen‐rich water on a rat model of periodontitis.Material & MethodsA ligature was placed around the maxillary molars for 4 weeks to induce periodontitis, and the animals were given drinking water with or without hydrogen‐rich water.ResultsThe rats with periodontitis which were treated with pure water showed a time‐dependent increase in serum ROS level. Compared with the rats without periodontitis, the periodontitis‐induced rats which were given pure water also showed polymorphonuclear leucocyte infiltration and alveolar bone loss at 4 weeks. Hydrogen‐rich water intake inhibited an increase in serum ROS level and lowered expression of 8‐hydroxydeoxyguanosine and nitrotyrosine in the periodontal tissue at 4 weeks. Such conditions prevented polymorphonuclear leucocyte infiltration and osteoclast differentiation following periodontitis progression. Furthermore, inflammatory signalling pathways, such as mitogen‐activated protein kinases, were less activated in periodontal lesions from hydrogen‐rich water‐treated rats as compared with pure water‐treated rats.ConclusionConsuming hydrogen‐rich water might be beneficial in suppressing periodontitis progression by decreasing gingival oxidative stress.
Stereoscopic Technique for Conversion of Radiographic Guide into Implant Surgical Guide
Authors: Chan Paul W. K. , Chik Fu‐Fai , Pow Edmond H. N. , Chow Tak‐Wah 
Published: October 2011

ABSTRACTPurpose: The aim of this study was to develop and evaluate a new stereoscopic technique for conversion of radiographic guide into surgical guide for dental implant placement.Materials and Methods: Ten partially dentate patients requiring 18 implants for tooth replacement were recruited. Radiographic guides were modified with the addition of index rods for double computed tomography scanning. Implant positions were planned with implant planning software, and the stereoscopic angulations were measured. The radiographic guides were converted into surgical guides using either a generic bench drill (Group A, n = 9) or a milling machine (Group B, n = 9). Stereolithographic surgical guides were also made for three patients (Group S, n = 5). Differences between the planned and actual angulations were tested by pair‐sample t‐test. Difference of mean angle deviation among groups was tested by Brown–Forsythe test. Differences were considered significant if p < .05.Results: Eighteen implant sites were successfully treated with the converted surgical guides. The mean angle deviation of Group A (1.3 ± 0.6°) was significantly greater than Group S (0.4 ± 0.6°), while no differences were found between Group B (0.9 ± 0.3°) and Group S. The linear error was greatest in Group A with 1.5 mm at the head and 1.8 mm at the apex of the implant.Conclusions: The use of this new stereoscopic technique appears to be an acceptable alternative method for converting radiographic guide into surgical guide.
Managing Peri‐Implant Bone Loss: Current Understanding
Authors: Aljateeli Manar , Fu Jia‐Hui , Wang Hom‐Lay 
Published: October 2011

ABSTRACTPurpose: With the improved macro‐ and micro‐designs, dental implants enjoy a high survival rate. However, peri‐implant bone loss has recently emerged to be the focus of implant therapy. As such, researchers and clinicians are in need of finding predictable techniques to treat peri‐implant bone loss and stop its progression.Materials and Methods: Literature search on the currently available treatment modalities was performed and a brief description of each modality was provided.Results: Numerous techniques have been proposed and none has been shown to be superior and effective in managing peri‐implant bone loss. This may be because of the complex of etiological factors acting on the implant‐supported prosthesis hence the treatment approach has to be individually tailored.Conclusion: Due to the lack of high‐level clinical evidence on the management of peri‐implant bone loss, the authors, through a literature review, attempt to suggest a decision tree or guideline, based on sound periodontal surgical principles, to aid clinicians in managing peri‐implantitis associated bone loss.
Evaluation of the occlusion vertical dimension of complete dentures after microwave disinfection
Authors: Basso Michael F. M. , Giampaolo Eunice T. , Machado Ana L. , Pavarina Ana C. , Vergani Carlos E. 
Published: October 2011

doi: 10.1111/j.1741‐2358.2011.00567.xEvaluation of the occlusion vertical dimension of complete dentures after microwave disinfectionObjectives:  An increase in occlusal vertical dimension (OVD) after microwave disinfection may result in the need for adjustments in the complete dentures. This in vitro study evaluated the increase in OVD of maxillary complete dentures submitted to microwave disinfection protocols.Material and methods:  Thirty sets of complete dentures were evaluated as follows: Group 1–15 sets had the maxillary complete dentures submitted to microwave disinfection (650 W/3 min), once a week, for 4 weeks. Group 2–15 sets had the maxillary complete dentures submitted to microwave disinfection (650 W/3 min), three times a week, for 4 weeks. The vertical dimension was measured with a micrometre (in mm) before disinfection protocols (baseline readings) and after each week of disinfection. Data were analysed using Wilcoxon and Friedman tests (α = 0.05).Results:  For Group 1, no significant difference was found between the increases in OVD and zero, and no significant difference was found between the weeks. For Group 2, the increases in OVD were significantly greater than zero, and the Friedman test showed that weeks 3 and 4 had significantly greater changes than week 1 and that week 4 had significantly greater change than week 2.Conclusion:  Microwave disinfection only promoted significant increase in OVD in Group 2, in which the values increased progressively.