bluuebunny/crossref_metadata_2025_split huggingface dataset:
Produksi personal care product berbasis atsiri untuk peningkatan kemandirian santri
Authors: Sukardi  , Sarosa Aji Hendra , Nirwana Wa Ode Cakra , Nurhadianty Vivi , Murlistyarini Sinta , Dewi Luthfi Kurnia 
Published: June 2024

Pondok Pesantren Darul Muttaqin yang terletak di Kecamatan Junrejo, Kota Batu merupakan pesantren dengan misi memberikan layanan pendidikan non-profit. Sampai saat ini biaya operasional pesantren masih sangat bergantung pada donatur. Satu-satunya bidang usaha yang dimiliki oleh Pesantren Darul Muttaqin adalah usaha bengkel Abdurrahman Bin Auf (ABA) untuk mendukung operasional pesantren tetapi belum dapat menjadikan pesantren dapat mandiri secara ekonomi. Oleh karena itu, pengelola pondok pesantren Darul Muttaqin sangat ingin mengembangkan usahanya di bidang lain. Metode pelaksanaan pengabdian menggabungkan aspek pelatihan dan penelitian lapangan untuk memberikan pemahaman kepada peserta tentang suatu topik tertentu. Kendala yang dihadapi adalah keterbatasan keterampilan, ilmu pengetahuan dan teknologi. Kegiatan Doktor Mengabdi bertujuan untuk memberikan pelatihan pembuatan produk personal care berbasis minyak atsiri lokal kepada mitra dalam bentuk produk sabun dan Sampo. Pelaksanaan dilakukan dalam tiga tahap, yaitu persiapan pelaksanaan, pemberian materi dan praktik pembuatan, serta monitoring dan evaluasi. Kegiatan telah terlaksana dengan baik, hal ini ditunjukkan dengan adanya respon dari peserta pelatihan sedikit menunjukkan ketidakpuasan, yaitu sebesar 4%, dan juga kepuasan sekitar 52%. Melalui kegiatan ini, Diharapkan ada keberlanjutan mengenai mengomersialisasikan hasil produksi dari pondok pesantren, sehingga dapat memberikan nilai tambah dalam ekonomi pondok pesantren.
Developing guidelines and a theory of change framework to inform rewilding application
Authors: Hawkins Sally , Convery Ian , Carver Steve 
Published: June 2024

IntroductionThere remain a number of debates and conflicts about the concept of rewilding which can be barriers to its application. Some of these conflicts stem from the variety of contextual interpretations of rewilding, leading to conflict between rewilding theories and approaches. Conclusions have also been drawn about rewilding based on limited case studies, so that emergent rewilding theories aren’t applicable to all rewilding projects, limiting their support in the field. Past theories have distinguished different types of rewilding, encouraging debate over the proposed methods, although in practice these approaches often share similar goals and use similar interventions. One barrier to achieving consensus in the practice of rewilding is that there are no clear guidelines for rewilding, and there are limited broad-scale studies focusing on how rewilding is practiced. This paper addresses this by offering the first broad study of rewilding guidelines and interventions.MethodsA grounded theory study was undertaken, using data sourced from rewilding organisations, case studies, and research. Expressions were coded in the data relating to intentions for how rewilding should be practiced and the interventions used.ResultsDrawing from these data, the paper offers three tools to guide rewilding practitioners: (1) an overview of guidelines for rewilding practice, (2) a list of interventions used in rewilding, considering them against rewilding goals, (3) a theory of change framework to guide rewilding application.DiscussionThe tools presented here will inform work towards IUCN rewilding guidelines. Several areas that require further consideration are highlighted. We hope that this initial study of application can improve agreement and collaboration among the rewilding community.
Necroptosis and immune infiltration in hypertrophic cardiomyopathy: novel insights from bioinformatics analyses
Authors: Hou Can , Fei Sifan , Jia Fang 
Published: June 2024

BackgroundHypertrophic Cardiomyopathy (HCM), a widespread genetic heart disorder, is largely associated with sudden cardiac fatality. Necroptosis, an emerging type of programmed cell death, plays a fundamental role in several cardiovascular diseases.AimThis research utilized bioinformatics analysis to investigate necroptosis's implication in HCM.MethodsThe study retrieved RNA sequencing datasets GSE130036 and GSE141910 from the Gene Expression Omnibus (GEO) database. It detected necroptosis-linked differentially expressed genes (NRDEGs) by reviewing both the gene set for necroptosis and the differently expressed genes (DEGs). The enriched signaling pathway of HCM was assessed using GSEA, while common DEGs were studied through Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Concurrently, the Protein-Protein Interaction network (PPI) proved useful for identifying central genes. CIBERSORT facilitated evaluating the correlation between distinct immune cell-type prevalence and NRDEGs by analyzing immune infiltration patterns. Lastly, GSE141910 dataset validated the expression ranks of NRDEGs and immune-cell penetration.ResultsThe investigation disclosed significant enrichment and activation of the necroptosis pathway in HCM specimens. Seventeen diverse genes, including CYBB, BCL2, and JAK2 among others, were identified in the process. PPI network scrutiny classified nine of these genes as central genes. Results from GO and KEGG enrichment analyses showed substantial connections of these genes to pathways pertaining to the HIF-1 signaling track, necroptosis, and NOD-like receptor signaling process. Moreover, an imbalance in M2 macrophage cells in HCM samples was observed. Finally, CYBB, BCL2, and JAK2 emerged as vital genes and were validated using the GSE141910 dataset.ConclusionThese results indicate necroptosis as a probable underlying factor in HCM, with immune cell infiltration playing a part. Additionally, CYBB, BCL2, JAK2 could act as potential biomarkers for recognizing HCM. This information forms crucial insights into the basic mechanisms of HCM and could enhance its diagnosis and management.
A machine learning radiomics based on enhanced computed tomography to predict neoadjuvant immunotherapy for resectable esophageal squamous cell carcinoma
Authors: Wang Jia-Ling , Tang Lian-Sha , Zhong Xia , Wang Yi , Feng Yu-Jie , Zhang Yun , Liu Ji-Yan 
Published: June 2024

BackgroundPatients with resectable esophageal squamous cell carcinoma (ESCC) receiving neoadjuvant immunotherapy (NIT) display variable treatment responses. The purpose of this study is to establish and validate a radiomics based on enhanced computed tomography (CT) and combined with clinical data to predict the major pathological response to NIT in ESCC patients.MethodsThis retrospective study included 82 ESCC patients who were randomly divided into the training group (n = 57) and the validation group (n = 25). Radiomic features were derived from the tumor region in enhanced CT images obtained before treatment. After feature reduction and screening, radiomics was established. Logistic regression analysis was conducted to select clinical variables. The predictive model integrating radiomics and clinical data was constructed and presented as a nomogram. Area under curve (AUC) was applied to evaluate the predictive ability of the models, and decision curve analysis (DCA) and calibration curves were performed to test the application of the models.ResultsOne clinical data (radiotherapy) and 10 radiomic features were identified and applied for the predictive model. The radiomics integrated with clinical data could achieve excellent predictive performance, with AUC values of 0.93 (95% CI 0.87–0.99) and 0.85 (95% CI 0.69–1.00) in the training group and the validation group, respectively. DCA and calibration curves demonstrated a good clinical feasibility and utility of this model.ConclusionEnhanced CT image-based radiomics could predict the response of ESCC patients to NIT with high accuracy and robustness. The developed predictive model offers a valuable tool for assessing treatment efficacy prior to initiating therapy, thus providing individualized treatment regimens for patients.
Long-term prognosis in patients with thymoma combined with myasthenia gravis: a propensity score-matching analysis
Authors: Zhao Kai , Liu Yiming , Jing Miao , Cai Wenhan , Jin Jiamei , Zhu Zirui , Shen Leilei , Wen Jiaxin , Xue Zhiqiang 
Published: June 2024

IntroductionWe aimed to assess the impact of myasthenia gravis (MG) on the long-term prognosis in patients with thymoma after surgery and identify related prognostic factors or predictors.MethodsThis retrospective observational study included 509 patients with thymoma (thymoma combined with MG [MG group] and thymoma alone [non-MG group]). Propensity score matching was performed to obtain comparable subsets of 96 patients in each group. A comparative analysis was conducted on various parameters.ResultsBefore matching, the 10-year survival and recurrence-free survival rates in both groups were 93.8 and 98.4%, and 85.9 and 93.4%, respectively, with no statistically significant difference observed in the survival curves between the groups (p &gt; 0.05). After propensity score matching, 96 matched pairs of patients from both groups were created. The 10-year survival and recurrence-free survival rates in these matched pairs were 96.9 and 97.7%, and 86.9 and 91.1%, respectively, with no statistical significance in the survival curves between the groups (p &gt; 0.05). Univariate analysis of patients with thymoma postoperatively revealed that the World Health Organization histopathological classification, Masaoka–Koga stage, Tumor Node Metastasis stage, resection status, and postoperative adjuvant therapy were potentially associated with tumor recurrence after thymoma surgery. Multivariate analysis demonstrated that the Masaoka–Koga stage and postoperative adjuvant therapy independently predicted the risk of recurrence in patients with thymoma after surgery.ConclusionThere was no difference in prognosis in patients with thymoma with or without MG. The Masaoka–Koga stage has emerged as an independent prognostic factor affecting recurrence-free survival in patients with thymoma, while postoperative adjuvant therapy represents a poor prognostic factor.
Thymol improves the growth performance of blue foxes by regulating the gut microbiota
Authors: Yuan Chongshan , Chen Siqi , Sun Rui , Ren Lili , Zhao Tiancheng , Wu Min , Zhang Aiwu 
Published: June 2024

IntroductionThe drawbacks of using antibiotics as feed additives for blue foxes have gradually become apparent; moreover, thymol has wide-spectrum antimicrobial activity and has the potential to replace antibiotics in various animals. However, there are few reports on the effects of thymol on blue foxes.MethodsThis study aimed to investigate the effects of different concentrations of thymol on the growth performance, apparent nutrient digestibility, serum biochemical indicators, intestinal morphology, and gut microbiota of blue foxes. Twenty-four male blue foxes (120 ± 5 d) of similar weight (6.05 ± 0.16 kg) were randomly divided into 4 groups. 0, 100, 200, and 300 mg/kg thymol were added to the basal diets of groups C, L, M, and H, respectively.ResultsCompared with those in the C group, the addition of 100 mg/kg thymol to the diet significantly increased organic matter (OM) digestibility, crude protein (CP) digestibility, immunoglobulin (Ig) A, IgM, the VH of the duodenum, the CD of the jejunum, the VH of the ileum, and the VH/CD of the ileum (P &lt; 0.05) and strongly significantly increased IgG (P &lt; 0.01). The addition of 200 mg/kg thymol to the diet increased the VH/CD of the duodenum (P &lt; 0.05). The addition of 300 mg/kg thymol to the diet significantly increased the VH and CD of the jejunum (P &lt; 0.05). The addition of 200 mg/kg and 300 mg/kg thymol to the diets increased the final weight (FW) (P &lt; 0.05). Adding 100 mg/kg thymol significantly increased the levels of interleukin-4 (IL-4) and catalase (CAT) compared with those in the other groups (P &lt; 0.05). 16S rRNA gene detection revealed that thymol can change the abundances of Bifidobacterium, Fusobacterium, Allobaculum, Streptococcus, Megasphaera, and Lactobacillus in the gut.ConclusionThe addition of thymol to diets can increase the abundance of Bifidobacterium, Fusobacterium, and Allobaculum, which may contribute to improving the growth performance of blue foxes.
Assessing the impact of storage conditions on RNA from human saliva and its application to the identification of mRNA biomarkers for asthma
Authors: Bhamidimarri Poorna Manasa , Fuentes David , Salameh Laila , Mahboub Bassam , Hamoudi Rifat 
Published: June 2024

Introduction: Human saliva was used to develop non-invasive liquid biopsy biomarkers to establish saliva as an alternate to blood and plasma in translational research. The present study focused on understanding the impact of sample storage conditions on the extraction of RNA from saliva and the RNA yield, to be applied in clinical diagnosis. In this study, genes related to asthma were used to test the method developed.Methods: Salivary RNA was extracted from three subjects using the Qiazol® based method and quantified by both spectrophotometric (NanoDrop) and fluorometric (Qubit®) methods. RNA integrity was measured using a bioanalyzer. Quantitative PCR was used to monitor the impact of storage conditions on the expression of housekeeping genes: GAPDH and β-actin, and the asthma related genes: POSTN and FBN2. In addition, an independent cohort of 38 asthmatics and 10 healthy controls were used to validate the expression of POSTN and FBN2 as mRNA salivary biomarkers.Results: Approximately 2 µg of total RNA was obtained from the saliva stored at 40°C without any preservative for 2 weeks showing consistent gene expression with RNA stored at room temperature (RT) for 48 h with RNAlater. Although saliva stored with RNAlater showed a substantial increase in the yield (110 to 234 ng/μL), a similar Cq (15.6 ± 1.4) for the 18s rRNA gene from saliva without preservative showed that the RNA was stable enough. Gene expression analysis from the degraded RNA can be performed by designing the assay using a smaller fragment size spanning a single exon as described below in the case of the POSTN and FBN2 genes in the asthma cohort.Conclusion: This study showed that samples stored at room temperature up to a temperature of 40°C without any preservative for 2 weeks yielded relatively stable RNA. The methodology developed can be employed to transport samples from the point of collection to the laboratory, under non-stringent storage conditions enabling the execution of gene expression studies in a cost effective and efficient manner.
Anti-saccade as a tool to evaluate cognitive impairment in vestibular migraine
Authors: Lu Lingmei , Ni Wenyu , Liu Yin , Sun Li , Li Fei 
Published: June 2024

BackgroundVestibular migraine (VM), an intricate subtype of migraine, amalgamates the dual attributes of migraine and vestibular disorders. In clinical settings, individuals with VM frequently articulate concerns regarding the manifestation of subjective cognitive impairment. This cognitive dysfunction is intricately linked with diminished mobility, heightened susceptibility to falls, and increased absenteeism in afflicted patients. Consequently, comprehending the features of cognitive impairment in VM patients holds potential clinical significance. The pursuit of rapid and objective methods for detection and assessment is foundational and prerequisite for efficacious cognitive management of VM patients.MethodsThe study encompassed 50 patients diagnosed with vestibular migraine and recruited 50 age-sex matched healthy controls. All participants underwent anti-saccade tasks, and cognitive evaluation was performed using the MMSE and MoCA to assess overall cognitive function. Additionally, RBANS scales were employed to measure specific cognitive domains.ResultsThe VM patients and normal controls demonstrated statistical parity in terms of age, gender, education, weight, and BMI, with no significant differences observed. Analysis of cognitive scores divulged a marked increase in the incidence of Mild Cognitive Impairment (MCI) in VM patients compared to Healthy Controls (HCs). Both MMSE and MoCA scores were notably lower in VM patients compared to their healthy counterparts. The RBANS cognitive test indicated significant impairment in immediate memory, visuospatial construction, language, attention, and delayed memory among VM patients. Notably, the Trail Making Test and Stroop Color-Word Test revealed compromised processing speed and executive function cognitive domains. The anti-saccadic task highlighted significantly elevated anti-saccadic latency and frequency of direction errors in vestibular migraine patients. Symptom severity, illness duration, and episode frequency in VM patients positively correlated with counter-scanning errors and negatively correlated with cognitive performance across diverse cognitive domains.ConclusionVM patients exhibit cognitive decline across multiple cognitive domains during the interictal period. This cognitive impairment may not be fully reversible, underscoring its potential clinical significance for cognitive management in VM patients. The sensitivity of anti-saccade tasks to the cognitive status of VM patients positions them as promising objective indicators for diagnosis, intervention, and evaluation of cognitive impairment effects in VM in future applications.
Cost-effectiveness of sotorasib as a second-line treatment for non-small cell lung cancer with KRASG12C mutation in China and the United States
Authors: Zhu Ya-Ning , Tang Meng , Sun Ke-Xin , Gao Bei , Shi Xian-Peng , Zhang Peng 
Published: June 2024

PurposeTo evaluate the cost-effectiveness of sotorasib versus docetaxel in non-small cell lung cancer (NSCLC) patients with KRASG12C mutation from the China and United States’social perspective.Materials and MethodsA Markov model that included three states (progression-free survival, post-progression survival, and death) was developed. Incremental cost-effectiveness ratio (ICER), quality-adjusted life-year (QALY), and incremental QALY were calculated for the two treatment strategies. One-way sensitivity analysis was used to investigate the factors that had a greater impact on the model results, and tornado diagrams were used to present the results. Probabilistic sensitivity analysis was performed with 1,000 Monte Carlo simulations. Assume distributions based on parameter types and randomly sample all parameter distributions each time., The results were presented as cost-effectiveness acceptable curves.ResultsThis economic evaluation of data from the CodeBreak 200 randomized clinical trial. In China, sotorasib generated 0.44 QAYL with a total cost of $84372.59. Compared with docetaxel, the ICER value of sotorasib was $102701.84/QALY, which was higher than willingness to pay (WTP), so sotorasib had no economic advantage. In the US, sotorasib obtained 0.35 QALY more than docetaxel, ICER was $15,976.50/QALY, which was more than 1 WTP but less than 3 WTP, indicating that the increased cost of sotorasib was acceptable. One-way sensitivity analysis showed that the probability of sotorasib having economic benefits gradually increased when the cost of follow-up examination was reduced in China. And there was no influence on the conclusions within the range of changes in China. When the willingness to pay (WTP) exceeds $102,500, the probability of sotorasib having cost effect increases from 0% to 49%.ConclusionSotorasib had a cost effect from the perspective in the United States. However, sotorasib had no cost effect from the perspective in China, and only when the WTP exceeds $102,500, the probability of sotorasib having cost effect increases from 0% to 49%.
DNA barcoding combined with high-resolution melting analysis to discriminate rhubarb species and its traditional Chinese patent medicines
Authors: Shen Luyi , Zhang Min , Qiu Yanmei , Yang Lin , Lu Yiwen , Li Hua , Zhang Leilei , Tang Fan , Wang Feijuan , Zhu Cheng , Bao Hexigeduleng , Ding Yanfei 
Published: June 2024

Introduction: Rhubarb is a frequently used and beneficial traditional Chinese medicine. Wild resources of these plants are constantly being depleted, meaning that rhubarb products have been subjected to an unparalleled level of adulteration. Consequentially, reliable technology is urgently required to verify the authenticity of rhubarb raw materials and commercial botanical drugs.Methods: In this study, the barcode-DNA high-resolution melting (Bar-HRM) method was applied to characterize 63 rhubarb samples (five Polygonaceae species: Rheum tanguticum, Rh. palmatum, Rh. officinale, Rumex japonicus and Ru. sp.) and distinguish the rhubarb contents of 24 traditional Chinese patent medicine (TCPM) samples. Three markers, namely ITS2, rbcL and psbA-trnH, were tested to assess the candidate DNA barcodes for their effectiveness in distinguishing rhubarb from its adulterants. A segment from ITS2 was selected as the most suitable mini-barcode to identify the botanical drug rhubarb in TCPMs. Then, rhubarbs and TCPM samples were subjected to HRM analysis based on the ITS2barcode.Results: Among the tested barcoding loci, ITS2 displayed abundant sites of variation and was effective in identifying Polygonaceae species and their botanical origins. HRM analysis based on the ITS2 mini-barcode region successfully distinguished the authenticity of five Polygonaceae species and eight batches of TCPMs. Of the 18 TCPM samples, 66.7 % (12 samples) were identified as containing Rh. tanguticum or Rh. officinale. However, 33.3 % were shown to consist of adulterants.Conclusions: These results demonstrated that DNA barcoding combined with HRM is a specific, suitable and powerful approach for identifying rhubarb species and TCPMs, which is crucial to guaranteeing the security of medicinal plants being traded internationally.
Analysis and experiment of stress concentration in penetration fuze buffer materials under stress wave incursion
Authors: Zhang An , Liu Peng , Zhang He 
Published: June 2024

To enhance the impact resistance of penetration fuze, this paper investigates the response of fuze buffer materials to stress waves and develops a model for stress wave transmission inside the fuze. The stress concentration impacts of different cell structures of Imitation Bamboo Type Penetration Fuze Buffer Protection Structure (IBS) under stress wave action are compared and analyzed. The paper elucidates the impact of different cell parameters on stress concentration impacts, establishes nonlinear fitting functions of Stress Concentration Factor (SCF) and cell parameters, and solves the prediction error. Based on the wave function expansion method, an expression for Dynamic Stress Concentration Factor (DSCF) when stress waves interact with the potting material is derived, and numerical results of DSCF around bubbles under different physical parameters are provided. Finally, dynamic impact tests are conducted on the combined buffer scheme of penetration fuze. Impact test results show that, under an initial velocity impact of 50 m/s, the overload peak attenuation rate is 39.42%, and under an initial velocity impact of 70 m/s, the overload peak attenuation rate is 32.87%. IBS can effectively protect the electronic components inside the fuze.