bluuebunny/crossref_metadata_2025_split huggingface dataset:
Hypophosphatemia Is Associated with Post-Operative Ileus After Right Colon Resection
Authors: Yu Allen T. , Malhotra Simran , Abeshouse Marnie , Yoo Esther , Sullivan Joseph , Huang Alex , Plietz Michael C. , Khaitov Sergey , Greenstein Alexander J. , Sylla Patricia , Hahn Sue J. 
Published: May 2024

AbstractBackgroundElectrolyte imbalances are known to contribute to intestinal ileus. However, the direct impact of hypophosphatemia on post-operative ileus (POI) is unknown.ObjectiveTo describe post-operative phosphate dynamics and if hypophosphatemia is associated with POI after a right colon resection.DesignComparative retrospective cohort studySettingsHigh-volume tertiary referral centerPatientsPatients who underwent right colon resection, which includes right hemicolectomies and ileocolic resections between 2020 and 2022.Main Outcome MeasuresPOI incidence, post-surgical phosphate dynamics, and post-operative phosphate deficit and recovery.ResultsA total of 396 patients were reviewed, where 68% of resections were for inflammatory bowel disease. Patients had a mean return of bowel function on POD 3.78 ± 1.45. 17.4% of patients overall had POI. Serum phosphate was the most dynamic post-operative electrolyte, with statistically significant differences between POI and non-POI on POD 1, 3, and 7 (p< 0.05). Serum phosphate recovery in patients with POI was impaired at 0.11 mg/dL/day versus 0.17 mg/dL/day (p< 0.001). Patients with POI had a phosphate deficit that persisted beginning on POD 2, with statistically significant deficits on POD 3-5 (p< 0.01), as well as POD 7 (p< 0.001). On multivariate analysis, a phosphate deficit on POD 3 (ORadj9.04, 95% CI 1.38-59.2), POD 5 (ORadj7.05, 1.13-44.1), and POD 7 (ORadj47.2, 2.98-749.4) were the only independent risk factors for POI.LimitationsGeneralizability of these findings may be limited outside of right colon resections.ConclusionsWe have established baseline phosphate dynamics in patients who undergo ileocolic anastomoses. We found POI was associated with a delayed serum phosphate recovery, as well as lower overall phosphate levels. Thus, a potential post-surgical window for intervention with timed phosphate repletion may have the potential to reduce post-operative ileus, need for nasogastric decompression, and ultimately decrease hospital length of stay.
Disparities in Lung Cancer Screening Utilization at Two Health Systems in the Southeastern US
Authors: Niranjan Soumya J , Rivers Desiree , Ramachandran Rekha , Edward Murrell J , Curry Kayleigh C , Mubasher Mohammed , Flenaugh Eric , Dransfield Mark T , Bae Sejong , Scarinci Isabel C 
Published: May 2024

ABSTRACTPurposeLow-dose computed tomography lung cancer screening is effective for reducing lung cancer mortality. It is critical to understand the lung cancer screening practices for screen-eligible individuals living in Alabama and Georgia where lung cancer is the leading cause of cancer death. High lung cancer incidence and mortality rates are attributed to high smoking rates among underserved, low income, and rural populations. Therefore, the purpose of this study: (1) to define sociodemographic and clinical characteristics of patients who were screened for lung cancer at an Academic Medical Center (AMC) in Alabama and a Safety Net Hospital (SNH) in Georgia.MethodsA retrospective cohort study of patient electronic health records who received lung cancer screening between 2015 to 2020 was performed to identify the study population and outcome variable measures. Chi-square tests and Student t-tests were used to compare screening uptake across patient demographic and clinical variables. Bivariate and multivariate logistic regressions determined significant predictors of lung cancer screening uptake.ResultsAt the AMC, 67,355 were identified as eligible for LCS and 1,129 were screened. In bivariate analyses, there were several differences between those who were screened and those who were not screened. Screening status in the site at Alabama varied significantly by age (P<0.01), race (P<0.001), marital status (P<0.01), smoking status (P<0.01) health insurance (P<0.01), median income (P<0.01), urban status (P<0.01) and distance from UAB (P<0.01). Those who were screened were more likely to have lesser comorbidities (2.31 vs. 2.53; P<0.001). At the SNH, 11,011 individuals were identified as screen-eligible and 500 were screened. In the site at Georgia, screening status varied significantly by race (P<0.01), health insurance (P<0.01), and distance from site (P<0.01). At the AMC, the odds of being screened increased significantly if the individual was a current smoker compared to former smoker (OR=3.21; P<0.01). At the SNH, the odds of being screened for lung cancer increased significantly with every unit increase in co-morbidity count (OR = 1.12; P=0.01)ConclusionThe study provides evidence that LCS has not reached all subgroups and that additional targeted efforts are needed to increase lung cancer screening uptake. Furthermore disparity was noticed between adults living closer to screening institutions and those who lived farther.
Post-translational digital data encoding into the genomes of mammalian cell populations
Authors: Callisto Alec , Strutz Jonathan , Leeper Kathleen , Kalhor Reza , Church George , Tyo Keith E.J. , Bhan Namita 
Published: May 2024

AbstractHigh resolution cellular signal encoding is critical for better understanding of complex biological phenomena. DNA-based biosignal encoders alter genomic or plasmid DNA in a signal dependent manner. Current approaches involve the signal of interest affecting a DNA edit by interacting with a signal specific promoter which then results in expression of the effector molecule (DNA altering enzyme). Here, we present the proof of concept of a biosignal encoding system where the enzyme terminal deoxynucleotidyl transferase (TdT) acts as the effector molecule upon directly interacting with the signal of interest. A template independent DNA polymerase (DNAp), TdT incorporates nucleotides at the 3’ OH ends of DNA substrate in a signal dependent manner. By employing CRISPR-Cas9 to create double stranded breaks in genomic DNA, we make 3’OH ends available to act as substrate for TdT. We show that this system can successfully resolve and encode different concentrations of various biosignals into the genomic DNA of HEK-293T cells. Finally, we develop a simple encoding scheme associated with the tested biosignals and encode the message “HELLO WORLD” into the genomic DNA of HEK-293T cells at a population level with 91% accuracy. This work demonstrates a simple and engineerable system that can reliably store local biosignal information into the genomes of mammalian cell populations.
Migration, proliferation, and elasticity of bladder cancer cells on lectin-coated surfaces
Authors: Luty Marcin , Szydlak Renata , Pabijan Joanna , Øvreeide Ingrid H , Prot Victorien E. , Zemła Joanna , Stokke Bjørn T. , Lekka Małgorzata 
Published: May 2024

AbstractThe alterations in migration, proliferation, and mechanics of cells observed during cancer progression can potentially be linked to enhanced tumor invasiveness. These properties are frequently attributed to the ability to form distant metastasis; however, the direct mutual connection between these properties is not always proven. Here, we studied the migratory, proliferative, and mechanical phenotype of three bladder cancer cells originating from various stages of cancer progression, i.e., non-malignant cell cancer of ureter (HCV29 cells), bladder carcinoma (HT1376 cells) and transitional bladder carcinoma (T24 cells). The results were linked with the organization of actin filaments because of their major role in cell migration. The results classified cells into non-malignant, non-invasive, and invasive, revealing the significant impact of actin filaments in bladder cancer invasion. Based on the reported changes in cancer cell glycosylation, the potential applicability of the observed cancer-related changes to identify invasive cells was demonstrated for the lectin-coated surfaces, which is the potential surface modification for biosensors.
Nuclear actin-dependent Meg3 expression suppresses metabolic genes by affecting the chromatin architecture at sites of elevated H3K27 acetylation levels
Authors: El Said Nadine Hosny , Abdrabou Wael , Mahmood Syed Raza , Venit Tomas , Idaghdour Youssef , Percipalle Piergiorgio 
Published: May 2024

AbstractThree-dimensional organization of the eukaryotic genome is directly affected by the nuclear β-actin pool that regulates enhancer function by affecting H3K27 acetylation levels. This actin-based mechanism, in turn, influences enhancer-dependent transcriptional regulation and plays a crucial role in driving gene expression changes observed upon compartment-switching. Using a combination of bulk RNA-seq and qPCR analyses performed on total RNA from WT mouse embryonic fibroblasts (MEFs), β-actin heterozygous (HET) MEFs, and β-actin KO MEFs, in this study we demonstrate that expression of several lncRNAs is directly affected by β-actin depletion. Among these lncRNAs, Meg3 expression increases in a β-actin dosage-dependent manner. Using ChIRP-seq, ChIRP-MS and f-RIP-qPCR, we show that β-actin depletion leads to alterations in Meg3 genomic association. It also leads to Meg3 enrichment at or close to gene regulatory sites including enhancers and promoters concomitantly with increased H3K27 acetylation levels. At these sites, specific Meg3 association with H3K27 acetylation leads to loss of promoter-enhancer interactions as revealed by the Activity by Contact (ABC) model that builds on RNA-seq, H3K27acetylation ChIP-seq, ATAC-seq and HiC-seq obtained in WT and β-actin KO MEFs. Results from metabolomics experiments in WT, HET and β-actin KO MEFs show these mechanisms contribute to the repression of genes involved in metabolic biosynthetic pathways for chondroitin, heparan, dermatan sulfate, and phospholipases, hence impacting their synthesis. We propose that at sites of actin-dependent increase in H3K27acetylation levels Meg3 interferes with promoter-enhancer interactions, potentially impairing local genome organization (or DNA looping) and negatively regulating gene expression.
Gauge fixing for sequence-function relationships
Authors: Posfai Anna , Zhou Juannan , McCandlish David M. , Kinney Justin B. 
Published: May 2024

Quantitative models of sequence-function relationships are ubiquitous in computational biology, e.g., for modeling the DNA binding of transcription factors or the fitness landscapes of proteins. Interpreting these models, however, is complicated by the fact that the values of model parameters can often be changed without affecting model predictions. Before the values of model parameters can be meaningfully interpreted, one must remove these degrees of freedom (called “gauge freedoms” in physics) by imposing additional constraints (a process called “fixing the gauge”). However, strategies for fixing the gauge of sequence-function relationships have received little attention. Here we derive an analytically tractable family of gauges for a large class of sequence-function relationships. These gauges are derived in the context of models with all-order interactions, but an important subset of these gauges can be applied to diverse types of models, including additive models, pairwise-interaction models, and models with higher-order interactions. Many commonly used gauges are special cases of gauges within this family. We demonstrate the utility of this family of gauges by showing how different choices of gauge can be used both to explore complex activity landscapes and to reveal simplified models that are approximately correct within localized regions of sequence space. The results provide practical gauge-fixing strategies and demonstrate the utility of gauge-fixing for model exploration and interpretation.Significance StatementComputational biology relies heavily on mathematical models that predict biological activities from DNA, RNA, or protein sequences. Interpreting the parameters of these models, however, remains difficult. Here we address a core challenge for model interpretation-the presence of ‘gauge freedoms’, i.e., ways of changing model parameters without affecting model predictions. The results unify commonly used methods for eliminating gauge freedoms and show how these methods can be used to simplify complex models in localized regions of sequence space. This work thus overcomes a major obstacle in the interpretation of quantitative sequence-function relationships.
Asymmetric binding of coactivator SRC1 to FXR-RXR and allosteric communication within the complex
Authors: Sheng Yanan , Guo Yaoting , Sun Mingze , Dong Yan , Yin Yue , Wang Yanwu , Peng Chao , Xu Yong , Wang Na , Liu Jinsong 
Published: May 2024

AbstractFarnesoid X receptor (FXR) is a promising target for treatment of metabolic associated fatty liver disease (MAFLD). In this study, we employed an integrative approach to investigate the interaction between FXR-RXRα-hSHP-1 complex and the entire coactivator SRC1-NRID (nuclear receptor interaction domain). We constructed a multi-domain model of FXR120-RXRα98-hSHP-1, highlighting the interface between FXR-DBD and LBD. Using HDX-MS, XL-MS, and biochemical assays, we revealed the allosteric communications in FXR-RXRα-hSHP-1 upon agonist and DNA binding. We then demonstrated that SRC1 binds only to the coactivator binding surface of FXR within the FXR-RXRα heterodimer, with the NR-box2 and NR-box3 of SRC1 as the key binding motifs. Our findings, which provide the first model of SRC1-NRID in complex with FXR-RXRα-hSHP-1, shed light on the molecular mechanism through which the coactivator asymmetrically interacts with nuclear receptors and provide structural basis for further understanding the function of FXR and its implications in diseases.
From fumble to flourish: how workplace attachment drives service recovery performance
Authors: Hassanie Souad , Karadas Georgiana , Uludag Orhan 
Published: May 2024

PurposeDrawing on the conservation of resources theory and attachment theory, this paper proposes that employee–organization relationship and satisfaction with work–life balance mediate the influence of workplace attachment on service recovery performance.Design/methodology/approachData collection was carried out through the distribution of questionnaires among a sample of healthcare providers from Lebanese private hospitals and analyzed via structural equation modeling.FindingsThe results indicate that workplace attachment has a significant impact on employee–organization relationship, satisfaction with work–life balance and service recovery performance. The findings further reveal that employee–organization relationship and satisfaction with work–life balance significantly influence service recovery performance and fully mediate the relationship between workplace attachment and service recovery performance.Originality/valueThis study is the first to investigate the mediating role of the employee–organization relationship and satisfaction with work–life balance between workplace attachment and service recovery performance. Over and above, this study is the first of its kind that the authors are aware of, being conducted in the healthcare sector, specifically in Lebanon. Based on the findings, the paper discusses the theoretical contributions, managerial implications and suggestions for further studies.
What are food media (messages)? A scoping review to clarify food media, food messages and food content in academic writing
Authors: Cuykx Isabelle , Lochs Caroline , Van Royen Kathleen , Vandebosch Heidi , Van den Bulck Hilde , Pabian Sara , de Backer Charlotte 
Published: May 2024

PurposeThis scoping review aims to explore how “food media”, “food messages” and “food content” are referred to in scholarly writing to enhance a shared understanding and comparability.Design/methodology/approachFollowing the PRISMA, ScR-guidelines, four scientific databases were screened on published manuscripts in academic journals, books and doctoral theses mentioning food media, content and messages within the prevalent meaning as in human communication.FindingsOf the 376 included manuscripts, only a small minority (n = 7) provided a conclusive definition of at least one of the three earlier-mentioned concepts; 40 others elucidated some aspects of food media, messages or content; however, they emphasized different and, sometimes even, contrasting aspects. In addition, the review explores in which disciplines the manuscripts mentioning food media, messages or content occur, which methodologies are used and what target groups and media are most common.Originality/valueBased on this aggregated information, a definition of food media, messages and content is proposed, aiming to enhance the comparability of diverse academic sources. This contribution invites scholars to critically reflect on the included media and content types when comparing studies on food media, messages or content.
Corporate social responsibility and labor investment efficiency: evidence from China
Authors: Wang Ting , Wang Jiangyuan 
Published: May 2024

PurposeWe expect to provide a complete theoretical framework and large sample evidence on the impact of corporate social responsibility (CSR) on the efficiency of labor investment. We also hope to provide micro-evidence based on labor investment behavior for the two-sided impact of corporate CSR behavior.Design/methodology/approachThis paper measures labor investment efficiency by estimating the difference between actual and expected net hiring of enterprises. CSR is measured on the basis of the CSR score of Chinese listed companies published by Hexun.com. A regression model is constructed to analyze the relationship between CSR and labor investment efficiency. Possible endogeneity problems are controlled by lagging independent variables, propensity score matching method and difference-in-difference method.FindingsResults show that CSR can improve labor investment efficiency by reducing over-hiring and under-hiring in emerging markets. The existence of the mediating effect of agency cost, information disclosure quality and employment fluctuation confirms that CSR improves labor investment efficiency through two mechanisms of corporate governance and labor market friction. The improvement effect of CSR on labor investment efficiency is more significant in non-state-owned, high CEO shareholding ratio and high-average urban wage enterprises.Originality/valueIn conclusion, our study is an important supplement to the existing research on the factors affecting labor investment efficiency. Our research conclusions will be helpful for enterprises in developing countries or enterprises in labor-intensive industries to improve labor investment inefficiency. The conclusion of the mechanism analysis in this paper provides more complete and reliable microscopic evidence for accurately identifying the specific path of CSR's impact on labor investment efficiency. This paper verifies the positive impact of CSR from the perspective of labor investment efficiency in the context of a developing country, which provides evidence for the theoretical conflicts related to CSR based on the effectiveness of enterprise labor investment decisions.
Cybersecurity for digital libraries: an interview with Emanuele Bellini
Authors: Bellini Emanuele , Tammaro Anna Maria 
Published: May 2024

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SWOT analysis of agricultural colleges for entrepreneurship: Delphi-Fuzzy and fuzzy hierarchical application
Authors: Tohidyan Far Somayeh , Rezaei-Moghaddam Kurosh 
Published: May 2024

PurposeThe present study aims to seek the strategic analysis of the entrepreneurship of agricultural colleges (AC).Design/methodology/approachIn terms of approach, this research was a combination of exploratory and hybrid methods. The present study was conducted in four stages. In the first stage, an open-ended questionnaire was designed to identify the strengths, weaknesses, opportunities and threats of entrepreneurship in AC (qualitative method). In the second stage, the Delphi-Fuzzy questionnaire was designed based on the results obtained from the first stage. In the third stage, the criteria of strengths, weaknesses, opportunities and threats of entrepreneurship of AC were analyzed based on the pairwise comparison (quantitative method) by the sample using a fuzzy hierarchical analysis process (FHAP). In the fourth stage, presented strategies were ranked based on pairwise comparison using FHAP.FindingsFrom the analysis of weaknesses, strengths, opportunities and threats facing AC for entrepreneurship, 12 strategies were presented in 4 groups of aggressive, conservative, competitive and defensive.Originality/valueThe literature review showed that no research has been done so far to identify strengths, weaknesses, opportunities and threats facing university entrepreneurship, especially AC. So the present study analyzes the weaknesses, strengths, opportunities and threats and proposes practical strategies for moving toward the formation of entrepreneurship AC. According to the gaps in providing SWOT of the AC, the results of this research can pave the way for policy makers and planners in this field.
Relational contracting in Nordic construction – a comparative longitudinal account of institutional field developments
Authors: Kadefors Anna , Aaltonen Kirsi , Gottlieb Stefan Christoffer , Klakegg Ole Jonny , Lahdenperä Pertti , Olsson Nils O.E. , Rosander Lilly , Thuesen Christian 
Published: May 2024

PurposeRelational contracting is increasingly being applied to complex and uncertain construction projects. However, it has proved hard to achieve stable performance and industry-level learning in this field. This paper employs an institutional perspective to analyze how legitimacy for relational contracting has been produced and challenged in Denmark, Finland, Norway and Sweden, including implications for dissemination and learning.Design/methodology/approachA collaborative case study design is used, where longitudinal accounts of the developments in relational contracting over more than 25 years in four Nordic countries were developed by scholars based in each country. The descriptions are underpinned by literature sources from research, practice and policy.FindingsThe countries share similar problem perceptions that have triggered the de-institutionalization of traditional contracting practices. Models and policies developed elsewhere are important sources of knowledge and legitimacy. Most countries have seen pendulum movements, where dissemination of relational contracting is followed by backlashes when projects fail to meet projected outcomes. Before long, however, relational contracting tends to re-emerge under new labels and in slightly new forms. Such a proliferation of concepts presents further obstacles to learning. Successful institutionalization is found to rely on realistic goals in combination with broad competence development at the organizational and industry levels.Practical implicationsIn seeking inspiration from other countries, policymakers should go beyond contract models to also consider strategies to manage industry-level learning.Originality/valueThe paper provides a unique longitudinal cross-country perspective on the field of relational contracting. As such, it contributes to the small stream of literature on long-term institutional change in the construction sector.