bluuebunny/crossref_metadata_2025_split huggingface dataset:
Essential role of p-selectin in endothelial progenitor cells-induced inhibition of platelet aggregation and thrombus formation
Authors: Saleh Haitham Abou 
Published: March 2012

AbstractWe have previously shown that Endothelial Progenitor Cells (EPCs) bind and inhibit platelet function and impair thrombus formation, but the surrounding mechanisms that regulate this process have not been fully described. Herein, we addressed the mechanistic action of EPCs on platelet-thrombus formation and we highlighted the role of platelet P-selectin in this process.EPCs were generated from human peripheral blood mononuclear cells after 10 days of culture on fibronectin in conditioned media. The impact of EPCs on platelet aggregation and thrombus formation was investigated in P-selectin deficient (P-sel-/-) and their wild-type counterpart (WT) mice. EPCs significantly impaired, in a concentration dependent-manner, collagen-induced whole blood platelet aggregation in WT mice; whereas in P-sel-/- mice, EPCs had no significant effect. Moreover, in ferric chloride-injured arterial thrombosis model, infusion of EPCs significantly reduced thrombus formation in WT, but not in P-sel-/- mice. Furthermore, the number of EPCs recruited within the thrombi and along the arterial wall was reduced in P-sel-/- mice as compared to WT mice, and the relative mass of thrombi generated in EPC-treated P-sel-/- mice was significantly larger than that in EPC-treated WT mice. EPCs impair platelet aggregation and reduce thrombus formation by a cellular mechanism implying binding to platelet P-selectin. These findings add new insights into the role of EPCs in the regulation of platelet function and thrombotic reaction during vascular repair.
Directional Photofluidization Lithography: Micro/Nanostructural Evolution by Photofluidic Motions of Azobenzene Materials
Authors: Lee Seungwoo , Kang Hong Suk , Park Jung‐Ki 
Published: March 2012

AbstractThis review demonstrates directional photofluidization lithography (DPL), which makes it possible to fabricate a generic and sophisticated micro/nanoarchitecture that would be difficult or impossible to attain with other methods. In particular, DPL differs from many of the existing micro/nanofabrication methods in that the post‐treatment (i.e., photofluidization), after the preliminary fabrication process of the original micro/nanostructures, plays a pivotal role in the various micro/nanostructural evolutions including the deterministic reshaping of architectures, the reduction of structural roughness, and the dramatic enhancement of pattern resolution. Also, DPL techniques are directly compatible with a parallel and scalable micro/nanofabrication. Thus, DPL with such extraordinary advantages in micro/nanofabrication could provide compelling opportunities for basic micro/nanoscale science as well as for general technology applications.
On the Frequency, Depth, and Duration of Sales at High–Low Pricing Supermarkets
Authors: Volpe Richard J. , Li Chenguang 
Published: March 2012

ABSTRACTThis study examines the phenomenon of advertised sales, or price discounts, at major high–low pricing (HLP) supermarkets in the United States. The authors measure and document price variability and movement using a unique and rich dataset on prices and sales drawn directly from supermarkets. Several of their key findings pertain directly to an influential study by Hosken and Reiffen (2004) on retail price variation. However, the authors’ estimate of price rigidity is lower than Hosken and Reiffen's while their estimate of sale frequency is higher. Moreover, the earlier study's key findings apply only to national brand products and not private labels. The authors also highlight the issue of sale duration, indicating that sales frequently run considerably longer than 1 or 2 weeks, to the point at which they argue they can no longer be considered temporary price reductions. Comparisons with alternative definitions of sale reveal that the empirical definition of a sale impacts the results significantly.
Toxicokinetic Profile of N‐(2‐Aminoethyl)ethanolamine in the Female Wistar Rat and Distribution into the Late Gestation Fetus and Milk
Authors: Moore Nigel P. , Saghir Shakil A. , Clark Amy J. , Hansen Steven C. , Carney Edward W. , Marshall Valerie A. , Rasoulpour Reza , Bartels Michael J. 
Published: March 2012

N‐(2‐aminoethyl)ethanolamine (AEEA) caused aneurysms of the great vessels in rats exposed in utero and during the first days post partum, exacerbated by postnatal treatment of the lactating dams (Moore et al., 2012. Birth Defects Res B Dev Reprod Toxicol [95:116‐122]). The purpose of this work was to examine the systemic availability of AEEA during gestation and early lactation. The absorption of AEEA was determined following oral administration to nonpregnant and pregnant female Wistar rats. A single dose administered by gavage (0.5 or 50 mg/kg) on gestation day 18 was rapidly and extensively (>90%) absorbed from the gastrointestinal tract (absorption t1/2 = 0.1–0.2 hr). Elimination from the plasma followed a biphasic pattern, with a rapid elimination phase (t1/2 α = 1.6–1.8 hr) followed by a slower phase (t1/2 β = 16.7–17.3 hr). Following repeated gavage administration during gestation day 17 to 19, 14C‐AEEA–derived radioactivity readily partitioned into the fetus and was evenly distributed therein, but cleared approximately twofold slower from the fetal blood and tissues than the maternal blood and chorioallantoic placenta. When administered to lactating dams during lactation days 1 to 12, 14C‐AEEA–derived radioactivity preferentially partitioned into the milk reaching levels that were between 1.6‐ and 2.5‐fold higher than the maternal blood. Although the concentration of AEEA equivalents in the maternal blood remained quite consistent, the concentration in the milk fell by almost 40% between lactation days 4 and 12, probably reflecting an increase in milk production over this same period. We confirm exposure of the offspring to AEEA both in utero and during lactation, but that AEEA does not appear to specifically concentrate in the great vessels.
Developmental engineering the kidney: Leveraging principles of morphogenesis for renal regeneration
Authors: Basu Joydeep , Ludlow John W. 
Published: March 2012

AbstractMultiple methodological approaches are currently under active development for application in tissue engineering and regenerative medicine of tubular and solid organs. Most recently, developmental engineering (TE/RM), or the leveraging of embryonic and morphological paradigms to recapitulate aspects of organ development, has been proposed as a strategy for the sequential, iterative de novo assembly of tissues and organs as discrete developmental modules ex vivo, prior to implantation in vivo. In this article, we focus on the kidney to highlight in detail how principles of developmental biology are impacting approaches to TE of this complex solid organ. Ultimately, such methodologies may facilitate the establishment of clinically relevant therapeutic strategies for regeneration of renal structure and function, greatly impacting treatment regimens for chronic kidney disease. Birth Defects Research (Part C) 96:30–38, 2012. © 2012 Wiley Periodicals, Inc.
A Machine Learning Approach for the Identification of Protein Secondary Structure Elements from Electron Cryo‐Microscopy Density Maps
Authors: Si Dong , Ji Shuiwang , Nasr Kamal Al , He Jing 
Published: March 2012

AbstractThe accuracy of the secondary structure element (SSE) identification from volumetric protein density maps is critical for de‐novo backbone structure derivation in electron cryo‐microscopy (cryoEM). It is still challenging to detect the SSE automatically and accurately from the density maps at medium resolutions (∼5–10 Å). We present a machine learning approach, SSELearner, to automatically identify helices and β‐sheets by using the knowledge from existing volumetric maps in the Electron Microscopy Data Bank. We tested our approach using 10 simulated density maps. The averaged specificity and sensitivity for the helix detection are 94.9% and 95.8%, respectively, and those for the β‐sheet detection are 86.7% and 96.4%, respectively. We have developed a secondary structure annotator, SSID, to predict the helices and β‐strands from the backbone Cα trace. With the help of SSID, we tested our SSELearner using 13 experimentally derived cryo‐EM density maps. The machine learning approach shows the specificity and sensitivity of 91.8% and 74.5%, respectively, for the helix detection and 85.2% and 86.5% respectively for the β‐sheet detection in cryoEM maps of Electron Microscopy Data Bank. The reduced detection accuracy reveals the challenges in SSE detection when the cryoEM maps are used instead of the simulated maps. Our results suggest that it is effective to use one cryoEM map for learning to detect the SSE in another cryoEM map of similar quality. © 2012 Wiley Periodicals, Inc. Biopolymers 97: 698–708, 2012.
Evidence of Myocardial Edema in Patients With Nonischemic Dilated Cardiomyopathy
Authors: Jeserich Michael , Föll Daniela , Olschewski Manfred , Kimmel Simone , Friedrich Matthias G. , Bode Christoph , Geibel Annette 
Published: March 2012

AbstractBackground:Nonischemic dilated cardiomyopathy (DCM) is associated with high mortality and morbidity. Cardiovascular magnetic resonance allows for the noninvasive assessment of function, morphology, and myocardial edema. Activation of inflammatory pathways may play an important role in the etiology of chronic DCM and may also be involved in the disease progression.Hypothesis:The purpose of our study was to assess the incidence of myocardial edema as a marker for myocardial inflammation in patients with nonischemic DCM.Methods:We examined 31 consecutive patients ( mean age, 57 ± 12 years) with idiopathic DCM. Results were compared with 39 controls matched for gender and age (mean age, 53 ± 13 years). Parameters of left ventricular function and volumes, and electrocardiogram‐triggered, T2‐weighted, fast spin echo triple inversion recovery sequences were applied in all patients and controls. Variables between patients and controls were compared using t tests for quantitative and χ2 tests for categorical variables.Results:Ejection fraction (EF) was 40.3 ± 7.8% in patients and 62.6 ± 5.0% in controls (P < 0.0001). In T2‐weighted images, patients with DCM had a significantly higher normalized global signal intensity ratio compared to controls (2.2 ± 0.6 and 1.8 ± 0.3, respectively, P = 0.0006), consistent with global myocardial edema. There was a significant but moderate negative correlation between signal intensity ratio in T2‐weighted images and EF (−0.39, P < 0.001).Conclusions:Evidence shows that myocardial edema is associated with idiopathic nonischemic DCM. Further studies are needed to assess the clinical and prognostic impact of these findings. Clin. Cardiol. 2012 DOI: 10.1002/clc.21979The authors have no funding, financial relationships, or conflicts of interest to disclose.
Fine‐needle cytology of Kaposi's sarcoma in an intramammary lymphnode: Report of one case
Authors: Fulciniti Franco , De Chiara Annarosaria , Apice Gaetano , Petrillo Antonella , Botti Gerardo , Feroce Florinda , Mozzillo Nicola 
Published: March 2012

AbstractKaposi's sarcoma (KS) is the most common human immunodeficiency virus (HIV) infection disease‐associated malignancy. It consists of an angiosarcomatous change of the epithelial and mucous membrane‐associated connective tissue not only in various sites, for example, skin, gastrointestinal system, lungs, and so on, but may also involve nonepithelial organs, such as lymphnodes. An unusual localization of KS to an intramammary lymphnode is reported here.The patient, an HIV‐negative 69‐year‐old woman with a clinical history of rheumatoid arthritis treated with hydrocortisone, had an 8‐month pathological history of biopsy‐proven Kaposi sarcoma of the skin with visceral extension (stomach and duodenum). The appearance of a well‐defined 23 × 20 mm2 breast nodule during chemotherapy elicited fine‐needle cytology to exclude breast carcinoma. Surgical excision confirmed the cytopathological diagnosis of Kaposis's sarcoma. Diagn. Cytopathol. 2012. © 2011 Wiley Periodicals, Inc.
Application of Fault Tree Analysis to Fuel Cell diagnosis
Authors: Yousfi Steiner N. , Hissel D. , Moçotéguy P. , Candusso D. , Marra D. , Pianese C. , Sorrentino M. 
Published: March 2012

AbstractReliability and lifetime are common issues for the development and commercialization of fuel cells technologies'. As a consequence, their improvement is a major challenge and the last decade has experienced a growing interest in activities that aims at understanding the degradation mechanisms and at developing fuel cell systems diagnosis tools.Fault Tree Analysis (FTA) is one of the deductive tools that allow “linking” an undesired state to a combination of lower‐level events via a “top–down” approach which is mainly used in safety and reliability engineering.The objective of this paper is to give an overview of the use and the contribution of FTA to both SOFC and PEFC diagnosis.
Extensive cell migration, axon regeneration, and improved function with polysialic acid‐modified Schwann cells after spinal cord injury
Authors: Ghosh Mousumi , Tuesta Luis M. , Puentes Rocio , Patel Samik , Melendez Kiara , El Maarouf Abderrahman , Rutishauser Urs , Pearse Damien Daniel 
Published: March 2012

AbstractSchwann cell (SC) implantation after spinal cord injury (SCI) promotes axonal regeneration, remyelination repair, and functional recovery. Reparative efficacy, however, may be limited because of the inability of SCs to migrate outward from the lesion‐implant site. Altering SC cell surface properties by overexpressing polysialic acid (PSA) has been shown to promote SC migration. In this study, a SCI contusion model was used to evaluate the migration, supraspinal axon growth support, and functional recovery associated with polysialyltransferase (PST)‐overexpressing SCs [PST‐green fluorescent protein (GFP) SCs] or controls (GFP SCs). Compared with GFP SCs, which remained confined to the injection site at the injury center, PST‐GFP SCs migrated across the lesion:host cord interface for distances of up to 4.4 mm within adjacent host tissue. In addition, with PST‐GFP SCs, there was extensive serotonergic and corticospinal axon in‐growth within the implants that was limited in the GFP SC controls. The enhanced migration of PST‐GFP SCs was accompanied by significant growth of these axons caudal to lesion. Animals receiving PST‐GFP SCs exhibited improved functional outcome, both in the open‐field and on the gridwalk test, beyond the modest improvements provided by GFP SC controls. This study for the first time demonstrates that a lack of migration by SCs may hinder their reparative benefits and that cell surface overexpression of PSA enhances the ability of implanted SCs to associate with and support the growth of corticospinal axons. These results provide further promise that PSA‐modified SCs will be a potent reparative approach for SCI. © 2012 Wiley Periodicals, Inc.
Transition‐Metal Catalysts for Controlled Radical Polymerization: A First Update
Authors: Fetzer Ludivine , Toniazzo Valerie , Ruch David , di Lena Fabio 
Published: March 2012

AbstractThis article, a follow‐up on a recent review co‐authored by one of us (Prog. Polym. Sci. 2010, 35, 959–1021) discusses the most recent discoveries in the field of metal‐catalyzed, reversible‐deactivation radical polymerization. Remarkable examples include the use of non‐toxic polyethylene glycols simultaneously as solvents and ligands for the iron‐catalyzed ATRP, nickel complexes that promote polymerization via a dual radical/migratory‐insertion pathway, and metalloenzymes as mediators of ARGET ATRP‐type reactions. A thorough and critical overview of the mechanistic details as well as the experimental conditions used to carry out the polymerizations are given together with an overview of the future challenges.
Coexistence of multiple and widespread cardiovascular complications in a patient with Marfan syndrome
Authors: Magnoni Marco , Figini Filippo , Piraino Daniela , Cianflone Domenico 
Published: March 2012

AbstractInherited connective tissue diseases such as Marfan syndrome are frequently associated with cardiovascular manifestations. Aortic involvement with dilation and dissection is the most common finding and the major cause of death in Marfan syndrome patients. We report the echocardiographic study of a 53‐year‐old male patient with uncommon coexistence of cardiovascular abnormalities typical of connective tissue disease at first clinical presentation in acute clinical setting: dissection of the descending aorta associated with severe mitral regurgitation due to leaflet flail and massive aortic insufficiency due to ascending aortic enlargement, leading to left ventricular dilation and dysfunction. © 2012 Wiley Periodicals, Inc. J Clin Ultrasound, 2013
Number of harvested lymph nodes is the main prognostic factor in Stage IIa colorectal cancer patients
Authors: La Torre Marco , Lorenzon Laura , Pilozzi Emanuela , Barucca Viola , Cavallini Marco , Ziparo Vincenzo , Ferri Mario 
Published: March 2012

AbstractBackgroundCurrent international guidelines on colorectal cancer (CRC) treatment suggest performing adjuvant chemotherapy only in Stage II patients presenting with high‐risk prognostic factors. Aim of the study was to a the impact of these parameters on the survival of Stage IIa CRC patients, focusing on the prognostic value of the number of harvested lymph nodes (NHLN).Patients and MethodsOut of 651 CRC patients undergoing surgical resection, 204 T3‐N0‐M0 were selected and reviewed. Univariate and multivariate survival analyses were adapted for the evaluation of the prognostic factors.ResultsThe 5 years actuarial DFS, DSS, and OS rates of patients with a NHLN >12 were significantly better compared to those of patients with a NHLN <12 (respectively: 72.8% vs. 50.4% P = 0.001; 82% vs. 56% P = 0.001; 78.5% vs. 53.1% P = 0.001). Multivariate analysis revealed that a NHLN >12 was the only independent parameter of statistical significance influencing DFS, DSS, and OS.ConclusionsAccording to our findings, the NHLN is the main predictor of survival in Stage IIa CRC patients. This would appear to suggest the need of a better stratification of Stage IIa CRC patients, sub‐dividing patients with more or less than 12 NHLN. J. Surg. Oncol. 2012; 106:469–474. © 2012 Wiley Periodicals, Inc.
Indeterminate pulmonary nodules in rectal cancer: A recommendation for follow‐up guidelines
Authors: Baek Se‐Jin , Kim Seon‐Hahn , Kwak Jung‐Myun , Cho Jae‐Sung , Shin Jae‐Won , Amar Azali Hafiz Yafee , Kim Jin 
Published: March 2012

AbstractBackgroundIncidental visualization of indeterminate pulmonary nodules is considered a clinical dilemma.MethodsWe identified patients for inclusion in this study by searching for the term “indeterminate nodules” in the radiology database of rectal cancer patients who underwent surgery. Patients with definite metastatic disease were excluded.ResultsIn total, 224 patients underwent chest computerized tomography (CT) and 59 of these patients had indeterminate pulmonary nodules detected more than twice by CT scan. Six patients (10.2%) were confirmed to have metastatic lesions on follow‐up evaluation. Pulmonary nodule size (P = 0.028), pathologic N status (P = 0.049), positive nodal status (P = 0.036) and the number of positive lymph nodes (P = 0.033) were significant risk factors for pulmonary metastasis. In the pulmonary metastasis group, the patients who had received adjuvant oxaliplatin‐based (FOLFOX4) chemotherapy had longer intervals to developing metastasis compared to patients who had not received it.ConclusionsIt is not necessary to perform excessive surveillance routinely for all rectal cancer patients who have indeterminate pulmonary lesions. Intensive follow‐up chest CT or other invasive diagnostic modalities should be considered only in patients with pulmonary nodules larger than 5.7 mm or positive nodal status. In addition, patients receiving adjuvant FOLFOX4 chemotherapy should be followed‐up for longer periods. J. Surg. Oncol. 2012; 106:481–485. © 2012 Wiley Periodicals, Inc.
Airway management for intubation in newborns with pierre robin sequence
Authors: Marston Alexander P. , Lander Timothy A. , Tibesar Robert J. , Sidman James D. 
Published: March 2012

AbstractObjectives/Hypothesis:To review airway management in Pierre Robin sequence (PRS) newborns undergoing general anesthesia and to determine if endotracheal intubation is safe in this population.Study Design:Case series and retrospective chart review at a tertiary children's hospital.Methods:PRS newborns who underwent endotracheal intubation or other airway intervention before 3 months of age between January 2000 and July 2011 were identified from a pediatric otolaryngology practice database. Indications for airway intervention, anesthetic management, method of intubation, and comorbid conditions were collected.Results:Thirty‐three PRS newborns were identified. Twenty had isolated PRS, and 13 had PRS related to a coexisting syndrome. Thirteen of 35 (37%) endotracheal intubations performed in PRS newborns prior to mandibular distraction osteogenesis were accomplished with direct laryngoscopy. The remaining 22 of 35 (63%) who failed intubation with direct laryngoscopy were intubated over a flexible fiberoptic bronchoscope. No significant difference was observed between the isolated and syndromic PRS newborns with regard to technique utilized for intubation. No patient required rescue laryngeal mask airway or emergent tracheotomy, and no case resulted in death.Conclusions:This series demonstrates that endotracheal intubation is safe and effective in PRS newborns. In patients who failed intubation with direct laryngoscopy, intubation over a flexible fiberoptic bronchoscope provided a reliable alternative method. Although airway management in PRS newborns poses a significant challenge, experienced otolaryngologists and anesthesiologists can successfully manage these difficult airway cases.
Is the use of topical fluorescein helpful for management of CSF leakage?
Authors: Ozturk Kayhan , Karabagli Hakan , Bulut Serap , Egilmez Meryem , Duran Mutlu 
Published: March 2012

AbstractObjectives/Hypothesis:We aimed to evaluate the effectiveness of topical intranasal fluorescence application together with high‐resolution computed tomography and magnetic resonance imaging and/or magnetic resonance cisternography for the preoperative diagnosis of cerebrospinal fluid (CSF) leakage and for intraoperative localization of CSF fistulas. We also assessed postoperative recurrence.Study Design:Descriptive study.Methods:Twenty‐four cases underwent endonasal endoscopic repair for CSF leakage. Following intranasal topical application of fluorescein 10% to the middle turbinate‐cribriform plate region and anterior wall of the sphenoid sinus, a color change of the fluorescein from yellow‐brown to green was accepted as evidence of CSF leakage. A nasoseptal flap, collagen matrix, or fascia lata and septal cartilage graft were used for reconstruction of the CSF fistula.Results:The use of topical fluorescein application was helpful in diagnosing CSF rhinorrhea preoperatively and locating the CSF fistula intraoperatively. One case had both ethmoid roof and sphenoid sinus defects, and this case underwent a second operation for closure of the sphenoid defect. All CSF leakages were successfully repaired by endoscopic endonasal surgery using a nasoseptal flap.Conclusions:Cases with traumatic CSF leakage pose a higher risk of meningitis than the cases with spontaneous CSF leakage. Topical application of fluorescein is highly specific and sensitive in identifying preoperative, intraoperative, and postoperative CSF leakage. It is a simple, quick, and sensitive technique. The surgeon should be vigilant for multiple defects.