bluuebunny/crossref_metadata_2025_split huggingface dataset:
VEGFR‐2 expression in carcinoid cancer cells and its role in tumor growth and metastasis
Authors: Silva Scott R. , Bowen Kanika A. , Rychahou Piotr G. , Jackson Lindsey N. , Weiss Heidi L. , Lee Eun Y. , Townsend Courtney M. , Evers B. Mark 
Published: May 2010

AbstractCarcinoid tumors are slow growing and highly vascular neuroendocrine neoplasms that are increasing in incidence. Previously, we showed that carcinoid tumors express vascular endothelial growth factor receptor 2 (VEGFR‐2) in the epithelial compartment of carcinoid tumor sections; yet, its role is not completely understood. The purpose of our study was to: (i) assess the expression of VEGFR‐2 in the novel human carcinoid cell line BON, (ii) to determine the role of PI3K/Akt signaling on VEGFR‐2 expression and (iii) to assess the effect of VEGFR‐2 on BON cell invasion, migration and proliferation. We found that, although VEGFR‐2 is expressed in BON cells, reduction in VEGFR‐2 expression actually enhanced proliferation, invasion, and migration of the BON cell line. Also, expression of VEGFR‐2 was inversely related to PI3K signaling. Carcinoid liver metastases in mice demonstrated decreased VEGFR‐2 expression. Furthermore, the expression of a truncated, soluble form of VEGFR‐2 (sVEGFR‐2), a protein demonstrated to inhibit cell growth, was detected in BON cells. The presence of VEGFR‐2 in the epithelial component of carcinoid tumors and in the BON cell line suggests an alternate role for VEGFR‐2, in addition to its well‐defined role in angiogenesis. The expression of sVEGFR‐2 may explain the inverse relationship between VEGFR‐2 expression and PI3K/Akt signaling and the inhibitory effect VEGFR‐2 has on BON cell proliferation, migration and invasion.
Aboard transthyretin: From transport to cleavage
Authors: Liz Márcia A. , Mar Fernando M. , Franquinho Filipa , Sousa Mónica M. 
Published: May 2010

AbstractTransthyretin (TTR) is a plasma and cerebrospinal fluid protein mainly recognized as the transporter of thyroxine (T4) and retinol. Mutated TTR leads to familial amyloid polyneuropathy, a neurodegenerative disorder characterized by TTR amyloid deposition particularly in peripheral nerves. Beside its transport activities, TTR is a cryptic protease and participates in the biology of the nervous system. Several studies have been directed at finding new ligands of TTR to further explore the biology of the protein. From the identified ligands, some were in fact TTR protease substrates. In this review, we will discuss the existent information concerning TTR ligands/substrates. © 2010 IUBMB IUBMB Life, 62(6): 433–439, 2010
Transmission infrared spectroscopy of whole blood – complications for quantitative analysis from leucocyte adhesion during continuous monitoring
Authors: Vahlsing Thorsten , Damm Uwe , Radhakrishna Kondepati Venkata , Leonhardt Steffen , Brendel Mathias D. , Wood Bayden R. , Heise H. Michael 
Published: May 2010

AbstractInfrared spectroscopy has been applied to analyse glucose and cellular components in whole blood with the aim of developing an online clinical diagnostic and monitoring modality. Leucocyte adsorption onto the CaF2 windows was observed over a period of several hours under continuous blood flow using a transmission cell of 30 μm path length. This build‐up of cellular material on the windows is responsible for diminishing the sample path length under the flow conditions chosen. The adsorption dynamics have been characterised and their impact on glucose monitoring is reported. For short‐term monitoring (<2 hours) a standard error of prediction of 11 mg/dL with human citrated blood samples from three different subjects was achieved. Furthermore, the leucocyte build‐up was also reported for porcine EDTA blood monitoring. Consequences and testing opportunities with regard to the first stages in the immune cell reaction to the exposure of body‐foreign materials to anticoagulated whole blood are discussed. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
A theoretical study on the catalytic mechanism of Mus musculus adenosine deaminase
Authors: Wu Xian‐Hui , Zou Guo‐Lin , Quan Jun‐Min , Wu Yun‐Dong 
Published: May 2010

AbstractThe catalytic mechanism of Mus musculus adenosine deaminase (ADA) has been studied by quantum mechanics and two‐layered ONIOM calculations. Our calculations show that the previously proposed mechanism, involving His238 as the general base to activate the Zn‐bound water, has a high activation barrier of about 28 kcal/mol at the proposed rate‐determining nucleophilic addition step, and the corresponding calculated kinetic isotope effects are significantly different from the recent experimental observations. We propose a revised mechanism based on calculations, in which Glu217 serves as the general base to abstract the proton of the Zn‐bound water, and the protonated Glu217 then activates the substrate for the subsequent nucleophilic addition. The rate‐determining step is the proton transfer from Zn‐OH to 6‐NH2 of the tetrahedral intermediate, in which His238 serves as a proton shuttle for the proton transfer. The calculated kinetic isotope effects agree well with the experimental data, and calculated activation energy is also consistent with the experimental reaction rate. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
Accurate prediction of enthalpies of formation for a large set of organic compounds
Authors: Liu Cun‐Xi , Wang Hai‐Xia , Li Ze‐Rong , Zhou Chong‐Wen , Rao Han‐Bing , Li Xiang‐Yuan 
Published: May 2010

AbstractThis article describes a multiparameter calibration model, which improves the accuracy of density functional theory (DFT) for the prediction of standard enthalpies of formation for a large set of organic compounds. The model applies atom based, bond based, electronic, and radical environmental correction terms to calibrate the calculated enthalpies of formation at B3LYP/6‐31G(d,p) level by a least‐square method. A diverse data set of 771 closed‐shell compounds and radicals is used to train the model. The leave‐one‐out cross validation squared correlation coefficient q2 of 0.84 and squared correlation coefficient r2 of 0.86 for the final model are obtained. The meanabsolute error in enthalpies of formation for the dataset is reduced from 4.9 kcal/mol before calibration to 2.1 kcal/mol after calibration. Five‐fold cross validation is also used to estimate the performance of the calibration model and similar results are obtained. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
The symptom checklist‐revised (SCL‐90‐R) is able to discriminate between simulation and fibromyalgia
Authors: Torres Xavier , Bailles Eva , Collado Antonio , Taberner Joan , Gutierrez Fernando , Peri Josep‐Maria , Arias Anna , Fullana Miquel‐Angel , Valdes Manuel 
Published: May 2010

AbstractThis study assessed the ability of the Symptom Checklist‐Revised (SCL‐90‐R) to discriminate between two groups of fibromyalgia patients (those who were about to begin a treatment including the explicit aim of returning to work and those who were initiating a legal procedure to obtain permanent disability compensation) and two groups of healthy volunteers (medical students and psychology graduates), who were asked to produce a symptomatic resemblance to a chronic pain disorder. Logistic regression analyses were applied to the SCL‐90‐R subscales and individual probabilistic indices of simulation were calculated. Results showed that the SCL‐90‐R was able to discriminate between healthy subjects and both groups of patients with a high sensitivity and specificity. The individual indices of simulation, which might be more useful in clinical practice than the comparison of the SCL‐90‐R profiles, also showed an appropriate level of accuracy. Inc. J Clin Psychol: 66: 1–17, 2010.
Functional mimicry of the acetylated C‐terminal tail of p53 by a SUMO‐1 acetylated domain, SAD
Authors: Cheema Amrita , Knights Chad D. , Rao Mahadev , Catania Jason , Perez Ricardo , Simons Brigitte , Dakshanamurthy Sivanesan , Kolukula Vamsi K. , Tilli Maddalena , Furth Priscilla A. , Albanese Christopher , Avantaggiati Maria Laura 
Published: May 2010

AbstractThe ubiquitin‐like molecule, SUMO‐1, a small protein essential for a variety of biological processes, is covalently conjugated to many intracellular proteins, especially to regulatory components of the transcriptional machinery, such as histones and transcription factors. Sumoylation provides either a stimulatory or an inhibitory signal for proliferation and for transcription, but the molecular mechanisms by which SUMO‐1 achieves such versatility of effects are incompletely defined. The tumor suppressor and transcription regulator p53 is a relevant SUMO‐1 target. Particularly, the C‐terminal tail of p53 undergoes both sumoylation and acetylation. While the effects of sumoylation are still controversial, acetylation modifies p53 interaction with chromatin embedded promoters, and enforces p53 apoptotic activity. In this study, we show that the N‐terminal region of SUMO‐1 might functionally mimic this activity of the p53 C‐terminal tail. We found that this SUMO‐1 domain possesses similarity with the C‐terminal acetylable p53 tail as well as with acetylable domains of other transcription factors. SUMO‐1 is, indeed, acetylated when conjugated to its substrates and to p53. In the acetylable form SUMO‐1 tunes the p53 response by modifying p53 transcriptional program, by promoting binding onto selected promoters and by favoring apoptosis. By contrast, when non‐acetylable, SUMO‐1 enforces cell‐cycle arrest and p53 binding to a different sets of genes. These data demonstrate for the first time that SUMO‐1, a post‐translational modification is, in turn, modified by acetylation. Further, they imply that the pleiotropy of effects by which SUMO‐1 influences various cellular outcomes and the activity of p53 depends upon its acetylation state. J. Cell. Physiol. 225: 371–384, 2010. © 2010 Wiley‐Liss, Inc.
Tuning of the characteristics of Au nanoparticles produced by solid target laser ablation into water by changing the irradiation parameters
Authors: Kalyva Maria , Bertoni Giovanni , Milionis Athanasios , Cingolani Roberto , Athanassiou Athanassia 
Published: May 2010

AbstractWe report the production of Au nanoparticles with different average sizes and size distributions, by laser ablation of a solid Au target into pure deionized water. Tuning laser parameters such as pulse duration, energy, and wavelength is possible to tune the size and the size distributions of the produced nanoparticles into the liquid. We demonstrate the possibility of production of highly monodispersed colloidal solutions, in which the average nanoparticle size ranges from 3 to 10 nm, using laser pulses of ns duration. Laser ablation using fs laser pulses can also produce very small nanoparticles, although a small population of bigger nanoparticles is always present. Low and high‐resolution transmission electron microscopy (TEM), in combination with UV–Vis spectroscopy have been employed for the characterization of our samples. Microsc. Res. Tech. 73:937–943, 2010. © 2010 Wiley‐Liss, Inc.
Application of 1,3‐azomethine ylides derived from α‐dicarbonyl compounds and L‐proline to the synthesis of pyrrolizidines
Authors: Felluga Fulvia , Forzato Cristina , Nitti Patrizia , Pitacco Giuliana , Valentin Ennio , Zangrando Ennio 
Published: May 2010

Abstract
magnified imageThe reaction of 2,3‐butanedione, ethyl pyruvate, and phenylglyoxal with β‐nitrostyrene and L‐proline in isopropanol at room temperature gives substituted pyrrolizidines, as a result of one‐pot three component reaction. On the contrary, a spiropyrrolizidine is formed from 1,2‐cyclohexanedione only when the reaction is carried out in refluxing isopropanol, whereas at room temperature, incorporation of the amine component into the products is not observed and bicyclo[3.2.1]octanones are formed, as a result of a tandem Michael‐Henry reaction. In this latter case, L‐proline acts as an organocatalyst, although with modest enantioselectivity. The stereochemistry of the products is given and the mechanism of formation of products is postulated, on the basis of stereochemical arguments. J. Heterocyclic Chem., (2010).
Multi‐technique investigation of archaeological pottery from Parma (Italy)
Authors: Bersani D. , Lottici P. P. , Virgenti S. , Sodo A. , Malvestuto G. , Botti A. , Salvioli‐Mariani E. , Tribaudino M. , Ospitali F. , Catarsi M. 
Published: May 2010

AbstractA ceramic factory with at least three kilns, active from the 14th century until the 17th century, was recently found close to the city walls of Parma (Italy). A series of measurements by different techniques was performed on pottery fragments belonging to the first and last production period. Micro‐Raman spectroscopy and scanning electron microscopy–energy dispersive spectroscopy were used to study the glazes and the painting materials, while time‐of‐flight neutron diffraction and X‐ray diffraction were used to characterize the ceramic bodies. The bodies are mainly composed by quartz, diopside, Na‐ and K‐rich alkali feldspars, gehlenite, calcite, and silica‐alumina glass; the red coloration is due to hematite. The glazes were obtained using a lead‐silica glass, with a fictive temperature of about 600 °C. The main pigments were identified. The ceramics were produced from illitic calcareous clays and annealed in an oxidizing atmosphere at an estimated temperature of 900–1000 °C. Different amounts of magnesium in ceramics of the 14th and 17th century suggest the use of different quarries. The white pigment in the 17th century ceramics, showing the Raman spectrum of nearly pure anatase, is due to a titanium‐bearing clay. Copyright © 2010 John Wiley & Sons, Ltd.
Micro‐Raman investigation of terra sigillata slips: a comparative study of central Italian and southern Gaul productions
Authors: Leon Y. , Lofrumento C. , Zoppi A. , Carles R. , Castellucci E. M. , Sciau Ph. 
Published: May 2010

AbstractTerra sigillata is certainly the most famous fine ware of the Roman period, and for this reason it has been a subject of interest for both the archeology and archeometry communities. X‐ray diffraction studies showed systematic differences in the mineral compositions between central Italian and south Gaul productions. All the slips contain hematite; the Gallic ones embody also a significant content of corundum, while the Italian productions contain a great proportion of spinel. Raman investigation fails to reveal directly these differences because the spectra are dominated by the hematite peaks. However, we originally demonstrate that, due to the occurrence of an upshift of bands, the Raman spectrum of these sigillata slips can be associated with a substituted hematite. This premise has been confirmed by comparing the crystallization of hematite in four annealed clays to that of the ancient slips. The results suggested that the presence of the strong additional Raman band at around 680 cm−1 present in both clays and ancient slips could be associated with the recrystallization of hematite, occurring above 750 °C. It could also be due to the magnetite content, but this hypothesis is not supported by X‐ray diffraction data. In addition, the reduction seems to take place only above 1050 °C, as suggested by the unveiling of hercynite as well as the darkening of the clay samples. Raman scattering is shown to be very sensitive to small variations in clays composition and to firing temperature, and it has been successfully used as an in situ sensitive probe for discriminating between Italian and south Gallic productions. Copyright © 2010 John Wiley & Sons, Ltd.
Randomized phase II study of gemcitabine plus radiotherapy versus gemcitabine, 5‐fluorouracil, and cisplatin followed by radiotherapy and 5‐fluorouracil for patients with locally advanced, potentially resectable pancreatic adenocarcinoma
Authors: Landry Jerome , Catalano Paul J. , Staley Charles , Harris Wayne , Hoffman John , Talamonti Mark , Xu Natalie , Cooper Harry , Benson Al B. 
Published: May 2010

AbstractPurposeA randomized phase II trial (E1200) was designed to assess toxicities and surgical resection rates in two neoadjuvant gemcitabine‐based chemoradiation regimens in patients with borderline resectable pancreatic cancer. The trial was terminated early due to poor accrual.Patients and MethodsPatients with borderline resectable adenocarcinomas of the pancreas were enrolled. Arm A patients (n = 10) received gemcitabine 500 mg/m2 IV weekly for 6 weeks, with radiation to 50.4 Gy followed by surgical resection. Arm B patients (n = 11) received preoperative gemcitabine 175 mg/m2 on days 1, 5, 29, and 33, cisplatin 20 mg/m2 on days 1–5 and 29–32, 5‐FU 600 mg/m2 on days 1–5 and 29–32, followed by radiation with continuous infusion 5‐FU 225 mg/m2 for 6 weeks. All patients received adjuvant gemcitabine 1,000 mg/m2 weekly × 3 for five cycles.ResultsThree patients in arm A, and two patients in arm B were resected. Hematologic toxicity was comparable between the two arms except more patients in arm B developed grade 3 or 4 thrombocytopenia than those in arm A. Arm B had fewer grade 1‐2 GI toxicities although more patients (45%) experienced grade 3–4 GI toxicity.ConclusionsThis phase II trial showed that both regimens were tolerable, and resectability and survival were comparable to previous studies. J. Surg. Oncol. 2010; 101:587–592. © 2010 Wiley‐Liss, Inc.
Pelvis and extremity osteosarcoma with similar tumor volume have an equivalent survival
Authors: Song Won Seok , Cho Wan Hyeong , Jeon Dae‐Geun , Kong Chang‐Bae , Kim Min Suk , Lee Jun Ah , Shawky Eid Ahmed , Kim Jae‐Do , Lee Soo‐Yong 
Published: May 2010

AbstractBackground and ObjectivesThe poor prognosis of pelvic osteosarcoma is well recognized, but the cause of this prognosis has not been well defined.MethodsWe analyzed the clinicopathologic characteristics of pelvic osteosarcoma, and their prognostic significances in a cohort of 41 patients, treated either surgically by ablation or non‐surgically. In a separate case‐controlled study, the 20 surgically treated osteosarcoma patients were matched with 38 patients with extremity osteosarcoma by tumor volume and age.ResultsThe 5‐year overall survival rates of the surgically and the non‐surgically treated pelvic osteosarcoma were 55.8 ± 13.0% and 4.8 ± 4.7%, respectively (P < 0.01). Non‐surgically managed pelvic osteosarcoma group were found to have a larger tumor volume (P = 0.02), bladder displacement and sacroiliac joint involvement (P < 0.01). The 5‐year metastasis free survival rates in the surgically treated pelvic and extremity osteosarcoma were no different (41.4 ± 12.8% vs. 48.6 ± 8.4%), and histologic responses to chemotherapy were similar in two groups.ConclusionsNo significant difference in survival or histologic response was found between the surgically treated pelvic and the extremity osteosarcoma with suitable tumor volume. Outcome of pelvis osteosarcoma corresponds to the poor survival of extremity osteosarcoma of high initial tumor burden. J. Surg. Oncol. 2010; 101:611–617. © 2010 Wiley‐Liss, Inc.
Preparation of molecular imprinted polymer with quaternary ammonium groups as recognition sites for separation of pig cyclophilin 18 and bovine serum albumin
Authors: Liu Huijuan , Han Ruifang , Feng Miao , Gao Junfei , Long Yi , Zhao Zhuo , Wang Ying , Mi Huaifeng 
Published: May 2010

AbstractWe introduce a new type of molecular imprinted polymer (MIP) with immobilized assistant recognition polymer chains (ARPCs) to create effective recognition sites. In this work, cloned pig cyclophilin 18 (pCyP18) and BSA were used as templates, respectively. The template protein was selectively assembled with ARPCs from the library which consists of numerous limited length polymer chains with randomly distributed recognition sites of the quaternary ammonium cationic groups and immobilizing sites. The assemblies of protein and ARPCs were adsorbed by macroporous microspheres and immobilized by cross‐linking polymerization. After removing the templates, the two kinds of synthesized MIPs were used to adsorb cloned pCyP18 and BSA from protein mixtures respectively and both showed high selectivity. It confirms that this new method is suitable to separate proteins of both low and high molecular weight. The extended experiment on adsorption of natural pCyP18 from cytosol shows that the obtained MIP using cloned protein as template can be used to enrich natural protein of low content.
Termination of pregnancy following prenatal diagnosis in France: how severe are the foetal anomalies?
Authors: Dommergues Marc , Mandelbrot Laurent , Mahieu‐Caputo Dominique , Boudjema Noel , Durand‐Zaleski Isabelle ,   and the ICI Group‐Club de médecine foetale
Published: May 2010

AbstractObjectiveTo determine how severe were the conditions leading to termination of pregnancy for foetal anomaly (TOPFA) in France.MethodsDetailed indications for TOPFA were extracted from medical charts.ResultsOf 2465 completed records, indications were: chromosomal anomalies n = 963 (39.1%), malformations of a single organ without chromosomal or genetic aetiologies n = 898 (36.4%), multiple malformations without chromosomal or genetic aetiologies n = 238 (9.7%), obstetrical complications n = 161 (6.5%), non‐chromosomal genetic diseases n = 158 (6.4%), foetal infections n = 21 (0.9%), unexplained severe oligohydramnios n = 20 (0.8%), foetal exposure to teratogenic agents n = 6. Overall, 33.3% of anomalies were lethal (e.g. anencephaly), 25.2% were expected to result in isolated mental retardation (e.g. Down) and 35.1% in substantial handicap (e.g. myelomeningocele). In 6.4% of cases, the anomaly was either of late onset (e.g. Huntington's disease) or with uncertain prognosis (e.g. agenesis of corpus callosum) or severity was debatable (e.g. single limb agenesis, sickle cell disease).ConclusionsAlthough there is no indisputable definition of which anomalies are ‘severe’, 93.6% of the decisions to terminate the pregnancy were made by women and professionals in reaction to anomalies which clearly were lethal or would lead to substantial physical and/or mental disabilities. Copyright © 2010 John Wiley & Sons, Ltd.
Comparative study of conducting polyaniline/copper and polyaniline/nickel composites in the presence of surfactants
Authors: Uygun Aysegul , Aslan Erhan 
Published: May 2010

AbstractPolyaniline/copper and polyaniline/nickel composites were synthesized in the presence and absence of surfactants using ammonium persulfate as an initiator. The structural and surface characteristics of the composites were studied and compared using different techniques. The interfacial interactions and thermal stability of the composites were characterized using X‐ray diffraction and thermogravimetric analysis. Surface properties were investigated using scanning electron microscopy and energy‐dispersed X‐ray spectroscopy. The results indicate significant changes in the morphological and physicochemical properties of the composites when incorporating surfactants. The surfactant‐induced surface characteristics also have an effect on conductivity. Copyright © 2010 Society of Chemical Industry