bluuebunny/crossref_metadata_2025_split huggingface dataset:
STAT3 is essential for the maintenance of neurosphere‐initiating tumor cells in patients with glioblastomas: A potential for targeted therapy?
Authors: Villalva Claire , Martin‐Lannerée Severine , Cortes Ulrich , Dkhissi Fatima , Wager Michel , Le Corf Amélie , Tourani Jean‐Marc , Dusanter‐Fourt Isabelle , Turhan Ali G. , Karayan‐Tapon Lucie 
Published: April 2010

AbstractGlioblastoma (GBM), the highest‐grade form of gliomas, is the most frequent and the most aggressive. Recently, a subpopulation of cells with stem cells characteristics, commonly named “tumor‐initiating stem cells” (TISCs) or “cancer stem cells” (CSCs) were identified in GBM. These cells were shown to be highly resistant to chemotherapeutic drugs and to ionizing radiations. Consequently, the knowledge of the signals that regulate the functions and survival of TISCs is crucial. In our work, we describe a neurosphere‐initiating cell (NS‐IC) assay to quantify TISC/CSCs from patients with GBM and show that these cells are tumorigenic in vivo. We demonstrate that the intracellular signal transducer and activator of transcription STAT3 is constitutively activated by phosphorylation preferentially on serine 727 in these cells. Moreover, we demonstrate that the selective inhibition of STAT3 by the chemical compound Stattic or by siRNA STAT3 abrogates TISC/CSC proliferation and NS‐IC suggesting that self‐renewal of GBM “stem‐like” cells depends on the presence of STAT3 for their maintenance. Finally, we show that inhibition of STAT3 by Stattic sensitizes TISC/CSCs to the inhibitory action of Temozolomide with a strong synergistic effect of both drugs. Overall, these results suggest that strategies focused on STAT3 inhibition are efficient at the level of “stem‐like” cells and could be of interest for therapeutic purposes in patients with malignant GBM.
Comparative hepatotoxicity of deoxynivalenol in rat, mouse and human liver cells in culture
Authors: Sahu Saura C. , O'Donnell Michael W. , Wiesenfeld Paddy L. 
Published: April 2010

AbstractThe present study was undertaken to assess,in vitro, the hepatotoxic potential of the food‐borne mycotoxin, deoxynivalenol (DON), using rat (Clone9 and MH1C1), mouse (NBL CL2) and human (WRL68 and HepG2) liver cells in culture. The cells were treated with DON for 24 h at 37 °C in 5% CO2at concentrations of 0–25 µg ml−1. Following the treatment period, the cells were assayed for biochemical markers of hepatotoxicity that included three independent cytotoxicity assays, oxidative stress and mitochondrial dysfunction. Concentration‐dependent cytotoxicity of DON was observed in each of the five different liver cells derived from three different species (rat, mouse and human) over the entire concentration range studied, beginning at 0.1 µg ml−1. At these concentrations DON did not induce a biologically significant increase in oxidative stress in these liver cells, and showed a significant decrease in the mitochondrial function only in the rat liver MH1C1 cells compared with the control. The results of thisin vitrostudy suggest that DON is a potential hepatotoxin for the rat, mouse and human liver cells in the concentration range tested in this study. The liver cells used in this study showed distinct endpoint‐sensitivity to DON related to the species. Published in 2010 by John Wiley and Sons, Ltd.
Phosphate and calcium are required for TGFβ‐mediated stimulation of ANK expression and function during chondrogenesis
Authors: Oca Paulina , Zaka Raihana , Dion Arnold S. , Freeman Theresa A. , Williams Charlene J. 
Published: April 2010

AbstractThe expression of ANK, a key player in biomineralization, is stimulated by treatment with TGFβ. The purpose of this study was to determine whether TGFβ stimulation of ANK expression during chondrogenesis was dependent upon the influx of calcium and phosphate into cells. Treatment of ATDC5 cells with TGFβ increased ANK expression during all phases of chondrogenic differentiation, particularly at day 14 (proliferation) and day 32 (mineralizing hypertrophy) of culture. Phosphate uptake studies in the presence and absence of phosphonoformic acid (PFA), a competitive inhibitor of the type III Na+/Pi channels Pit‐1 and Pit‐2, indicated that the stimulation of ANK expression by TGFβ required the influx of phosphate, specifically by the Pit‐1 transporter, at all phases of differentiation. At hypertrophy, when alkaline phosphatase is highly expressed, inhibition of its activity with levamisole also abrogated the stimulatory effect of TGFβ on ANK expression, further illustrating that Pi availability and uptake by the cells is necessary for stimulation of ANK expression in response to TGFβ. Since previous studies of endochondral ossification in the growth plate have shown that L‐type calcium channels are essential for chondrogenesis, we investigated their role in the TGFβ‐stimulated ANK response in ATDC5 cells. Treatment with nifedipine to inhibit calcium influx via the L‐type channel Cav1.2 (α1C) inhibited the TGFβ stimulated increase in ANK expression at all phases of chondrogenesis. Our findings indicate that TGFβ stimulation of ANK expression is dependent upon the influx of phosphate and calcium into ATDC5 cells at all stages of differentiation. J. Cell. Physiol. 224: 540–548, 2010. © 2010 Wiley‐Liss, Inc.
Contrast‐enhanced ultrasound features of primary splenic lymphoma
Authors: Sutherland Tom , Temple Faye , Hennessy Oliver , Lee Wai‐Kit 
Published: April 2010

AbstractWe report a case of primary splenic lymphoma incidentally detected on abdominal ultrasound as a well‐defined homogenous hypoechoic splenic mass. Further characterization with contrast‐enhanced ultrasound showed the mass to enhance homogenously during the arterial phase to a degree similar to that of the normal splenic parenchyma followed by rapid washout becoming hypoenhanced on delayed phase. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound, 2010
Ultrasound demonstration of Struthers' ligament
Authors: Camerlinck Michael , Vanhoenacker Filip M. , Kiekens Gunther 
Published: April 2010

AbstractThe supracondylar process is a bony spur at the anteromedial surface of the distal humerus. It is associated with the Struthers' ligament, running from its apex to the medial epicondyle. Symptoms may occur due to fracture and entrapment of nerves (most frequently the median nerve) and/or blood vessels underneath the structures. We report a case in which we used MRI and high‐frequency ultrasound to investigate the supracondylar process, the Struthers' ligament, and its surrounding structures. Ultrasound is a useful alternative to MRI during the workup of potential entrapment syndromes. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound 38:499–502, 2010
A survival case of painless chronic type A aortic dissection with a history of stroke and anticoagulant use
Authors: Tugcu Aylin , Yildirimturk Ozlem , Demiroglu I.C. Cemsid , Aytekin Saide 
Published: April 2010

AbstractWe report the case of a patient with completely painless chronic aortic dissection, who presented to another hospital with a left hemiparesia 3 months ago and received anticoagulation therapy with a diagnosis of ischemic stroke. Most of her symptoms had resolved when she presented to our outpatient clinic except for numbness of her left hand and dysphasia. Physical examination found a diastolic murmur at the left sternal border and a bruit over the right carotid artery. Transthoracic echocardiography and carotid sonography demonstrated aortic dissection with extension into the internal right carotid artery and severe aortic regurgitation. Surgery was performed successfully and the patient was discharged. This case emphasizes that the diagnosis of a completely painless aortic dissection with only neurologic symptoms at presentation can be extremely difficult and should always be considered as a cause of ischemic stroke to avoid catastrophic antithrombolytic or anticoagulation therapy. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound 38:454–456, 2010
Effects of exposure to the xenoestrogen octylphenol and subsequent transfer to clean water on liver and gonad ultrastructure during early development of Zoarces viviparus embryos
Authors: Jespersen Åse , Rasmussen Tina H. , Hirche Majken , Sørensen Kristine J.K. , Korsgaard Bodil 
Published: April 2010

AbstractFemale eelpouts (Zoarces viviparus L.) are exposed during early pregnancy to nominal concentrations of 100 µg/L of 4‐tert‐octylphenol (OP) or 0.5 µg/L of 17β‐estradiol (E2). Effects on maternal metabolism and on liver and gonad development in embryos were examined and compared with controls (C) during exposure and after transfer to clean water (depuration). In the mother fish, significantly higher concentrations of plasma vitellogenin (vtg) and calcium were found in the two exposed groups, when compared with the C group after exposure and depuration. When compared, however, with the respective values after exposure, vtg had decreased significantly after depuration. The hepatosomatic index was normalized after depuration. In both exposed groups, the hepatocytes were rounded and not distinctly polygonal as in the controls. The amount of glycogen was considerably less while the number of mitochondria increased, and the rER significantly proliferated after exposure as well as after depuration. The gonads of nine of more than 28 embryos in the group treated with OP exhibited a number of abnormalities as compared with the normal gonad development in both sexes. Feminization of the male gonads in the exposed specimens and a number of histopathological features were observed in all the abnormal gonads, whereas reliable male features, such as formation of seminiferous tubules or spermioduct, were not observed. This study showed that 4t‐tert‐OP and 17β‐estradiol exert estrogenic effects during very early development of the embryos and that depuration had a positive effect on the motherfish and her embryos. J. Exp. Zool. 313A:399–409, 2010. © 2010 Wiley‐Liss, Inc.
Spatiotemporal changes in metallothionein gene expression during embryogenesis in the wall lizard Podarcis sicula
Authors: Simoniello Palma , Motta Chiara Maria , Scudiero Rosaria , Trinchella Francesca , Filosa Silvana 
Published: April 2010

AbstractLizard embryos are nutritionally independent from their environment. During the early phases of oogenesis, the egg prepares for development by storing reserve organelles, proteins, and RNAs sufficient to allow the zygote to transform into a juvenile. This preparation also includes the storage of metallothionein (MT) transcripts. This study investigated the localization of these transcripts by in situ hybridization throughout Podarcis sicula developmental stages. Our data show that MT expression undergoes shifts in both regional and cellular localization. MT transcripts were detected early in the central nervous system, later in tissues implicated in metabolic processes. Results are discussed highlighting differences in lizard embryonic spatial and temporal MT expression compared with piscine, amphibian, and mammalian embryos. We hypothesize that, under natural conditions, the nutritionally closed system represented by the lizard egg protects the developing embryo from an unwanted excess of metals. This mechanism would make MT expression and accumulation in detoxifying organs in developing animals unnecessary until hatching and food intake begins. Conversely, the presence of MT transcripts during brain development may ensure the correct final architecture of this organ. J. Exp. Zool. 313A:410–420, 2010. © 2010 Wiley‐Liss, Inc.
Involvement of a novel p38 mitogen‐activated protein kinase in larval metamorphosis of the polychaete Hydroides elegans (Haswell)
Authors: Wang Hao , Qian Pei‐Yuan 
Published: April 2010

AbstractHydroides elegans is a common marine fouling organism in most tropical and subtropical waters. The life cycle of H. elegans includes a planktonic larval stage in which swimming larvae normally take 5 days to attain competency to settle. Larval metamorphosis marks the beginning of its benthic life; however, the endogenous molecular mechanisms that regulate metamorphosis remain largely unknown. In this study, a PCR‐based suppressive subtractive hybridization (SSH) library was constructed to screen the genes expressed in competent larvae but not in precompetent larvae. Among the transcripts isolated from the library, 21 significantly matched sequences in the GenBank. Many of these isolated transcripts have putative roles in the reactive oxygen species (ROS) signal transduction pathway or in response to ROS stress. A putative novel p38 mitogen‐activated protein kinase (MAPK), which was also isolated with SSH screen, was then cloned and characterized. The MAPK inhibitors assay showed that both p38 MAPK inhibitors SB202190 and SB203580 effectively inhibited the biofilm‐induced metamorphosis of H. elegans. A cell stressors assay showed that H2O2 effectively induced larval metamorphosis of H. elegans, but the inductivity of H2O2 was also inhibited by both SB inhibitors. The catalase assay showed that the catalase could effetely inhibit H. elegans larvae from responding to inductive biofilm. These results showed that the p38 MAPK‐dependent pathway plays critical role in controlling larval metamorphosis of the marine polychaete H. elegans, and the reactive oxygen radicals produced by biofilm could be the cue inducing larval metamorphosis. J. Exp. Zool. (Mol. Dev. Evol.) 314B:390–402, 2010. © 2010 Wiley‐Liss, Inc.
Developmental plasticity links local adaptation and evolutionary diversification in foraging morphology
Authors: Young Rebecca L. , Badyaev Alexander V. 
Published: April 2010

AbstractDevelopmental plasticity is thought to reconcile the constraining role of natural selection in maintaining local adaptation with evolutionary diversification under novel conditions, but empirical documentations are rare. In vertebrates, growth and development of bones is partially guided by contractions of attached musculature and such muscle activity changes progressively through embryonic development from sporadic motility to direct functional effects. In species with short generation times, delayed skull maturation extends the guiding effects of muscle activity on formation of foraging morphology into adulthood, providing an opportunity to directly examine the links between plasticity of bone development, ecological adaptations, and evolutionary diversification in foraging morphology. In this case, the morphological consequences of inputs due to local functional requirements should be evident in adaptive divergence across taxa. Here we provide evidence that epigenetic regulation of bone growth in Soricid shrews may enable both development of local adaptations and evolutionary divergence in mandibular morphology. We contrast the effects of muscle stimulation on early‐ vs. late‐maturing components of, foraging apparatus to show that the morphology of late‐maturing components is more affected by functional requirements than are early‐ossifying traits. Further, the divergence in foraging morphology across shrew species occurs along the directions delineated by inductive effects of muscle loading and bite force on bone formation in late‐maturing but not early‐maturing mandible components within species. These results support the hypothesis that developmental plasticity can link maintenance of local adaptations with evolutionary diversification in morphology.J. Exp. Zool. (Mol. Dev. Evol.) 314B:434–444, 2010. © 2010 Wiley‐Liss, Inc.
Collaborating with patients and their families
Authors: Johnson Bev , Ford Dan , Abraham Marie 
Published: April 2010

AbstractPatient‐ and family‐centered care is a healthcare approach that emphasizes collaboration among patients, families, and healthcare providers. The key concepts of patient‐ and family‐centered care can be applied in any healthcare setting to enhance patient safety, reduce the risk of medical errors, improve risk management processes, and create an organizational culture supportive of risk managers and open, honest communication.
Implementation of a patient‐friendly medication schedule to improve patient safety within a healthcare system
Authors: Fredericks Jodi E. , Bunting Jr. Robert F. 
Published: April 2010

AbstractPreventable adverse drug events have a direct impact on the well‐being of patients. The creation and implementation of a patient‐friendly daily medication schedule improved the way care is delivered at Memorial Healthcare System. The staff collaborated with patients and families and empowered them with the knowledge and tools needed to make their healthcare safer. Patient and family participation, a critical component of patient‐ and family‐centered care, is a vital part of making healthcare safer. This tool enhances communication with patients and family members and enables patients to better understand the medications they receive while hospitalized. An additional welcomed byproduct is the prevention of potential medication errors.
Apoptosis markers related to pathogenesis of pediatric chronic hepatitis C virus infection: M30 mirrors the severity of steatosis
Authors: Valva Pamela , De Matteo Elena , Galoppo María C. , Gismondi María I. , Preciado María V. 
Published: April 2010

AbstractApoptosis involvement in liver damage related to hepatitis C virus (HCV) chronic infection has been suggested. Although liver biopsy represents the gold standard for evaluating disease severity, non‐invasive tests are a growing medical need. The aim of this study was to detect apoptosis markers in liver and serum from pediatric HCV‐infected patients and to assess its utility to predict liver damage progression. Twenty‐three patients were included. Liver biopsies were used for histological analysis as well as for immunodetection of a viral protein (NS3) and apoptosis markers (activated caspase‐3 [casp‐3a], caspase‐generated CK‐18 fragment [M30] and TUNEL). M30 was quantified in paired serum and biopsy samples. NS3 correlated both with casp‐3a (r = 0.83, P < 0.0001) and TUNEL (r = 0.61, P < 0.0017). Casp‐3a and TUNEL also displayed a correlation (r = 0.56, P = 0.005). Both NS3 and casp‐3a were associated with fibrosis stage (P = 0.03). Serum M30 [median: 122.15 UL‐1 (86.68–794.58)] was higher in patients than in controls [median: 81.44 UL‐1 (41.17–129.30)], (P < 0.0001). M30 showed a correlation with steatosis, and indeed it was linked to severe grade (P = 0.004). In children, HCV would be involved in liver damage through apoptosis induction. The apoptosis markers detected reflect liver injury. Serum M30 might be useful as a marker to detect the extent of liver steatosis. J. Med. Virol. 82:949–957, 2010. © 2010 Wiley‐Liss, Inc.
Hantaviruses in small mammals and humans in the coastal region of Zhejiang Province, China
Authors: Zhang Yong‐Zhen , Lin Xian‐Dan , Shi Nan‐Feng , Wang Wen , Liao Xiao‐Wei , Guo Wen‐Ping , Fan Fei‐Neng , Huang Xiu‐Min , Li Ming‐Hui , Li Mei‐Fu , Chen Yi , Chen Xiao‐Ping , Wang Shao‐Bin , Fu Zhen F. , Plyusnin Alexander 
Published: April 2010

AbstractTo gain more insights into the epidemiology of hantaviruses in the coastal region of Zhejiang Province, China, the morbidity and mortality of hemorrhagic fever with renal syndrome (HFRS) were analyzed in two coastal areas: Cixi (hilly terrain) and Wenzhou (mountainous terrain). More HFRS cases have been reported in Cixi than in Wenzhou. Annual incidence rate of HFRS in Cixi had been on the level of approximately 1.5/100,000 from 1968 (when the first HFRS case was reported) to 2007, with the highest incidence rate of 8.54/100,000 in 1999. The annual incidence rate in Wenzhou has been relatively low, less than 0.5/100,000 since the first HFRS case was reported in 1981. A total of 461 rodents and 199 shrews were captured in these two areas. Hantavirus antibodies were detected in 16 of 241 (6.64%) Rattus norvegicus and 13 of 122 (10.66%) R. flavipectus. Interestingly, hantavirus antigens were identified in 6 of 196 (3.06%) Suncus murinus. Genetic analysis showed that partial M and S segment sequences recovered from rats in the two regions belong to Seoul virus (SEOV) and can be assigned into two genetic lineages. SEOV variants that belong to these two lineages of viruses are distributed widely in China and have been found outside China. As most trapped rodents were rats and SEOV was the only hantavirus detected, these results suggested that SEOV plays an important role in human hantavirus infections. They also reinforce the need for vigilance in preventing HFRS caused by hantaviruses in the coastal region. J. Med. Virol. 82:987–995, 2010. © 2010 Wiley‐Liss, Inc.
No evidence of family history as a risk factor for herpes zoster in patients with post‐herpetic neuralgia
Authors: Gatti A. , Pica F. , Boccia M.T.Y. , De Antoni F. , Sabato A.F. , Volpi A. 
Published: April 2010

AbstractLittle is known about reactivation of latent varicella zoster virus as herpes zoster in individuals with no underlying immunosuppression. Risk factors include age, sex, ethnicity, exogenous boosting of immunity from varicella contacts, underlying cell‐mediated immune disorders, mechanical trauma, psychological stress, and immunotoxin exposure. An association between herpes zoster and family history of zoster has been proposed. A case‐control study involving patients affected by post‐herpetic neuralgia, which usually follows more severe acute herpes zoster, was performed. The patients with post‐herpetic neuralgia were enrolled at the Pain Clinic of the Policlinico Tor Vergata in Rome, Italy, within 1 year from the onset of acute zoster. The controls matched for sex and age were chosen among healthy subjects without a history of herpes zoster presenting at the Internal Medicine Outpatient Clinic for hypertension in the same time period. All the participants in the study gave informed consent and were interviewed by medically trained and blinded investigators using a questionnaire. Similar proportions of the patients and the controls reported a family history of herpes zoster irrespective of the degree of relationship, i.e., 17.4% and 18.2%, respectively, by analyzing only the first‐degree relatives [RR 1.03 (CI 95%: 0.78–1.37)], and 28.4% and 29.6%, respectively, by analyzing the total number of relatives [RR 1.03 (CI 95%: 0.81–1.31)]. Further and larger prospective cohort studies are needed to ascertain whether a family history of herpes zoster is really an independent predictor of zoster in different geographical settings. J. Med. Virol. 82:1007–1011, 2010. © 2010 Wiley‐Liss, Inc.