bluuebunny/crossref_metadata_2025_split huggingface dataset:
EpCAM‐ and EGFR‐targeted selective gene therapy for biliary cancers using Z33‐fiber‐modified adenovirus
Authors: Kawashima Rei , Abei Masato , Fukuda Kuniaki , Nakamura Kiminori , Murata Takehide , Wakayama Mariko , Seo Emiko , Hasegawa Naoyuki , Mizuguchi Hiroyuki , Obata Yuichi , Hyodo Ichinosuke , Hamada Hirofumi , Yokoyama Kazunari K. 
Published: November 2010

AbstractA critical issue in adenovirus (Ad)‐based cancer gene therapy is to improve the specificity of gene delivery to cancer cells for better efficacy and safety. We explored methods of retargeting Ad vectors for selective gene therapy of human biliary cancers using the Ad incorporating an IgG Fc‐binding motif (Z33) from the Staphylococcus protein A (Ad‐FZ33) combined with tumor‐specific antibodies. Flow cytometry analysis revealed high‐expression levels of epithelial cell adhesion molecule (EpCAM) and epidermal growth factor receptor (EGFR) on human biliary cancer cells. Ad‐FZ33 expressing LacZ combined with antibodies against EpCAM or EGFR, followed by β‐gal assay, demonstrated highly efficient gene transduction in these biliary cancer cells, compared to the treatment with control antibody or without antibody. Ad‐FZ33 expressing uracil phosphoribosyl transferase (UPRT), an enzyme which greatly enhances the toxicity of 5‐fluorouracil (FU), combined with antibodies against EpCAM or EGFR, remarkably enhanced the sensitivity of biliary cancer cells to 5‐FU. By contrast, the treatment did not affect the 5‐FU sensitivity of the cells not expressing EpCAM or EGFR including normal hepatocytes. Finally, treatments with the UPRT‐expressing Ad‐FZ33 with antibodies against EpCAM or EGFR, followed by 5‐FU administration, significantly suppressed the growth of biliary cancer xenografts in nude mice. These results indicate that the gene therapy mediated by the Z33 fiber modified Ad with anti‐EpCAM or anti‐EGFR antibodies offers a potentially effective therapeutic modality against biliary cancers.
In vitro study on Zr‐based bulk metallic glasses as potential biomaterials
Authors: Wang Y. B. , Zheng Y. F. , Wei S. C. , Li M. 
Published: November 2010

AbstractWith pure Ti and pure Zr as controls, the corrosion resistance, ion release behavior, and in vitro biocompatibility of Be‐containing Zr41Ti14Cu12Ni10Be23 bulk metallic glass (BMG) (LM1), Zr44Ti11Cu10Ni10Be25 BMG (LM1b), and Be‐free Zr57Nb5Cu15.4Ni12.6Al10 BMG (LM106) were investigated in terms of electrochemical measurements in simulated body fluid (SBF) with pH value 7.4 and artificial saliva (AS) with pH value 6.3, and 3‐[4,4‐dimethylthiazol‐2‐yl]‐2,5‐diphenyltetrazolium bromide (MTT) assay using L929 and NIH3T3 cells, aiming to assess the feasibility of Zr‐based BMGs as potential biomaterial. It was found that LM1b showed superior corrosion resistance to LM106 and LM1 in both SBF and AS, and comparable with pure Ti and pure Zr. After 7200 s immersion, a two‐layer structure oxide film was formed on LM1, LM1b, and pure Zr surfaces, while one‐layer structure oxide film was formed on LM106 and pure Ti surfaces. The pitting corrosion potentials of LM1b were much higher than that of LM1, LM106, and pure Zr, resulting in very few ions releasing into the electrolytes. No Be ion could be detected but a little amount of Cu ion was detected for LM106, LM1, and LM1b after immersion in Dulbecco's modified Eagle's medium for 72 h at 37°C. The indirect cytotoxicity results show that LM106, LM1, and LM1b extracts had no cytotoxicity to L929 and NIH3T3 cells. The direct cytotoxicity results show that cells could adhere well on the Zr‐based BMG surface as in pure Ti and Zr. Lower cell proliferation rate of LM106 and LM1 is observed when compared with LM1b, which was found to be caused by Cu ion releasing rather than by Be ion. © 2010 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2011.
Antimicrobial properties of silver nanoparticles synthesized by bioaffinity adsorption coupled with TiO2 photocatalysis
Authors: Chen Enzan , Su Haijia , Tan Tianwei 
Published: November 2010

AbstractBACKGROUND: On the basis of effective bioaffinity adsorption of Ag+, silver nanoparticles (Ag NPs) were synthesized on the surface of chitosan‐TiO2 adsorbent (CTA) by TiO2 photocatalysis for crystal growth.RESULTS: Among the microstructure characterizations of the resulting silver nanoparticles‐ loaded chitosan‐TiO2 adsorbent (Ag‐CTA), X‐ray diffraction (XRD) and scanning electron microscopy (SEM) equipped with energy dispersive X‐ray (EDX) revealed the formation of metallic Ag on the CTA, which was further confirmed by the surface plasmon resonance of Ag NPs in the UV‐visible absorption spectrum. The underlying mechanism behind the formation of Ag NPs on the CTA by TiO2 photoreduction was studied by Fourier transform infrared (FTIR) spectroscopy. The distinctive feature of Ag‐CTA after adsorption was the highly efficient antimicrobial activity in inactivating different test strains. In the case of Escherichia coli, 1.50 mg 1.67 wt% Ag‐CTA could totally inhibit 1.0–1.2 × 107 colony forming units (CFU) in 100 mL nutrient medium, which was superior to that previously reported.CONCLUSIONS: CTA effectively adsorbed the precious metal ion Ag+ onto active imprinting sites on the adsorbent and then exerted efficient antimicrobial effects against diverse microbes. This research will be useful for designing a novel CTA‐based wastewater treatment for multi‐functional performance. Copyright © 2010 Society of Chemical Industry
Gross morphology, histology, and ultrastructure of the alimentary system of Ricinulei (Arachnida) with emphasis on functional and phylogenetic implications
Authors: Talarico Giovanni , Lipke Elisabeth , Alberti Gerd 
Published: November 2010

AbstractRicinuleid functional mouthparts are the cucullus, the chelicerae, the pedipalps, and the labrum. These structures are movably jointed to the rest of the prosoma, most likely protruded upon hydrostatic hemolymph pressure and retracted by prosomal muscles. Seta‐like protrusions from the labrum and the pedipalpal coxae form a sieve‐like filter inside the preoral cavity and the mouth. Although the tip of the labrum can be elevated upon muscle constriction, ingestion of large, solid food particles is unlikely. The mouth has a crescent‐shaped cross section. The cuticle‐lined, also crescent‐shaped pharynx is equipped with a large dilator muscle but lacks antagonistic constrictor muscles. It represents a precerebral sucking pump. The triangular to Y‐shaped, cuticle‐lined esophagus is equipped with constrictor and dilator muscles. Its posterior part represents a postcerebral sucking pump. Four blind ending diverticula ramify from the anterior prosomal part of the entodermal midgut tube. Two of these diverticula remain inside the prosoma and form few short branches. The other two extend through the pedicel into the opisthosoma and ramify and coil there. A stercoral pocket protrudes ventrally out of the midgut tube. The most distal part of the midgut tube is modified into a contractile rectal gland. Its secretions may have defensive or physiological functions. A short anal atrium is formed by the cuticle‐lined ectodermal hindgut which opens at the end of the three‐segmented metasoma. The telescoping segments of the metasoma are protruded by hemolymph pressure and retracted by muscles. J. Morphol., 2011. © 2010 Wiley‐Liss, Inc.
Corallite wall and septal microstructure in scleractinian reef corals: Comparison of molecular clades within the family Faviidae
Authors: Budd Ann F. , Stolarski Jarosław 
Published: November 2010

AbstractRecent molecular phylogenies conflict with traditional scleractinian classification at ranks ranging from suborder to genus, challenging morphologists to discover new characters that better agree with molecular data. Such characters are essential for including fossils in analyses and tracing evolutionary patterns through geologic time. We examine the skeletal morphology of 36 species belonging to the traditional families Faviidae, Merulinidae, Pectiniidae, and Trachyphylliidae (3 Atlantic, 14 Indo‐Pacific, 2 cosmopolitan genera) at the macromorphological, micromorphological, and microstructural levels. Molecular analyses indicate that the families are not monophyletic groups, but consist of six family‐level clades, four of which are examined [clade XV =Diploastrea heliopora; clade XVI =Montastraea cavernosa; clade XVII (“Pacific faviids”) = Pacific faviids (part) + merulinids (part) + pectiniids (part) +M. annulariscomplex; clade XXI (“Atlantic faviids”) = Atlantic faviids (part) + Atlantic mussids].Comparisons among molecular clades indicate that micromorphological and microstructural characters (singly and in combination) are clade diagnostic, but with two exceptions, macromorphologic characters are not. The septal teeth of “Atlantic faviids” are paddle‐shaped (strong secondary calcification axes) or blocky, whereas the septal teeth of “Pacific faviids” are spine‐shaped or multidirectional. Corallite walls in “Atlantic faviids” are usually septothecal, with occasional trabeculothecal elements; whereas corallite walls in “Pacific faviids” are usually trabeculothecal or parathecal or they contain abortive septa. Exceptions include subclades of “Pacific faviids” consisting of a)CaulastraeaandOulophyllia(strong secondary axes) and b)Cyphastrea(septothecal walls).Diploastreahas a diagnostic synapticulothecal wall and thick triangular teeth;Montastraea cavernosais also distinct, possessing both “Pacific faviid” (abortive septa) and “Atlantic faviid” (paddle‐shaped teeth) attributes. The development of secondary axes is similar in traditional Atlantic faviids and mussids, supporting molecular results placing them in the same clade. Subclades of “Pacific faviids” reveal differences in wall structure and the arrangement and distinctiveness of centers of rapid accretion. J. Morphol. 272:66–88, 2011. © 2010 Wiley‐Liss, Inc.
Expression analysis of proline rich 15 (Prr15) in mouse and human gastrointestinal tumors
Authors: Meunier Dominique , Patra Kalicharan , Smits Ron , Hägebarth Andrea , Lüttges Angela , Jaussi Rolf , Wieduwilt Matthew J. , Quintanilla‐Fend Leticia , Himmelbauer Heinz , Fodde Riccardo , Fundele Reinald H. 
Published: November 2010

AbstractProline rich 15 (Prr15), which encodes a protein of unknown function, is expressed almost exclusively in postmitotic cells both during fetal development and in adult tissues, such as the intestinal epithelium and the testis. To determine if this specific expression is lost in intestinal neoplasias, we examined Prr15 expression by in situ hybridization (ISH) on mouse intestinal tumors caused by different gene mutations, and on human colorectal cancer (CRC) samples. Prr15/PRR15 expression was consistently observed in mouse gastrointestinal (GI) tumors caused by mutations in the Apc gene, as well as in several advanced stage human CRCs. In contrast, no Prr15 expression was detected in intestinal tumors derived from mice carrying mutations in the Smad3, Smad4, or Cdkn1b genes. These findings, combined with the fact that a majority of sporadic human CRCs carry APC mutations, strongly suggest that the expression of Prr15/PRR15 in mouse and human GI tumors is linked, directly or indirectly, to the absence of the APC protein or, more generally, to the disruption of the Wnt signaling pathway. © 2010 Wiley‐Liss, Inc.
Motivation and self‐regulation assessments: Professional practices and needs of school psychologists
Authors: Cleary Timothy J. , Gubi Aaron , Prescott Michelle V. 
Published: November 2010

AbstractThe current study examined differences between urban and suburban school psychologists in terms of their motivation and self‐regulation assessment beliefs, practices, and professional development needs. Using a sample of 96 urban and 47 suburban school psychologists sampled from 22 school districts in a Midwestern state, we found that the two groups were not differentiated across most survey items. Of primary practical importance, however, urban and suburban practitioners encountered motivation and self‐regulation referrals on a relatively frequent basis, perceived these processes to be valuable, and were strongly interested in professional development training. Interestingly, both groups of practitioners indicated that they do not routinely evaluate these processes in youth and were unfamiliar with many commercially available and research‐oriented motivation/self‐regulation self‐report scales. These results are consistent with the premise that there is a disconnect between the types of assessments that practitioners perceive to be valuable and the extent to which they actually engage in those practices. © 2010 Wiley Periodicals, Inc.
Atmospheric plasma treatment of pre‐electrospinning polymer solution: A feasible method to improve electrospinnability
Authors: Shi Quan , Vitchuli Narendiran , Nowak Joshua , Lin Zhan , Guo Bingkun , Mccord Marian , Bourham Mohamed , Zhang Xiangwu 
Published: November 2010

AbstractThe electrospinnability of polyethylene oxide (PEO) was manipulated by atmospheric plasma treatment of pre‐electrospinning solutions. Conductivity, viscosity, and surface tension of PEO solutions increased after plasma treatment, and the plasma effect remained longer when the solution concentrate increased. Both untreated and treated solutions were then electrospun, and the morphology of the resultant nanofibers was observed by SEM. Atmospheric plasma treatment improved the electrospinnability of PEO solutions and led to less beads and finer diameter distribution in the resultant nanofibers. Additionally, plasma treatment of the pre‐electrospinning solutions affected the crystal structure of resultant nanofibers. These results suggest that atmospheric plasma treatment is a feasible approach to improve the electrospinnability of polymer solutions and can used to control the structure of electrospun nanofibers. © 2010 Wiley Periodicals, Inc. J Polym Sci Part B: Polym Phys, 2011
Replication of CNTNAP2 association with nonword repetition and support for FOXP2 association with timed reading and motor activities in a dyslexia family sample
Authors: Peter Beate , Raskind Wendy H. , Matsushita Mark , Lisowski Mark , Vu Tiffany , Berninger Virginia W. , Wijsman Ellen M. , Brkanac Zoran 
Published: November 2010

Abstract
Two functionally related genes, FOXP2 and CNTNAP2, influence language abilities in families with rare syndromic and common nonsyndromic forms of impaired language, respectively. We investigated whether these genes are associated with component phenotypes of dyslexia and measures of sequential motor ability. Quantitative transmission disequilibrium testing (QTDT) and linear association modeling were used to evaluate associations with measures of phonological memory (nonword repetition, NWR), expressive language (sentence repetition), reading (real word reading efficiency, RWRE; word attack, WATT), and timed sequential motor activities (rapid alternating place of articulation, RAPA; finger succession in the dominant hand, FS-D) in 188 family trios with a child with dyslexia. Consistent with a prior study of language impairment, QTDT in dyslexia showed evidence of CNTNAP2 single nucleotide polymorphism (SNP) association with NWR. For FOXP2, we provide the first evidence for SNP association with component phenotypes of dyslexia, specifically NWR and RWRE but not WATT. In addition, FOXP2 SNP associations with both RAPA and FS-D were observed. Our results confirm the role of CNTNAP2 in NWR in a dyslexia sample and motivate new questions about the effects of FOXP2 in neurodevelopmental disorders.
Justifying and Criticizing the Removals of Antiquities in Ottoman Lands: Tracking the Sigeion Inscription
Authors: Thomasson Fredrik 
Published: November 2010

AbstractThis article attempts to widen the debate on the removal of antiquities from the Ottoman Empire around 1800. The removals are often seen in an Anglo-French perspective with the result that other voices are erased, both those of the local populations and of other foreign observers. I show that objects that now neglected were once highly valued by both local inhabitants and collectors, and that their removals were repeatedly resisted. I suggest that a more subtle interaction occurred between collectors and the local populations than hitherto has been recognized. While the local populations were accused of various “superstitious” practices—often conveniently related to objects coveted by European collectors—I propose that the removers were not uninfluenced by these practices. By introducing testimonies from Swedish observers that were critical of the removals before such critiques became frequent, I also question the stereotype of a common European attitude toward the Ottoman Empire. I discuss how such critique was related to their status as third-party observers from a nation without power or museums. By investigating the arguments of both collectors and critics, I propose that many positions in the debate today were already present at the time of the removals. Following the history of a less famous object serves to highlight aspects of early European collecting and expansion in the Eastern Mediterranean that are often overlooked in the debate on ownership and restitution claims.
Spatial structure of nuclear factors involved in sex determination in the gynodioecious Thymus vulgaris L.
Authors: Manicacci D. , Atlan A. , Couvet D. 
Published: November 2010

AbstractTheoretical models have shown that the maintenance of high frequencies of females in gynodioecious species can be explained by the spatial structure of cytoplasmic and nuclear genes involved in sex determination. Whereas spatial structure of cytoplasmic factors which cause male‐sterility has been studied in several taxa, that of nuclear factors that restore male‐fertility (restoration factors) has received little attention. In this paper, we estimate spatial variation in the frequency of restoration factors associated with different cytoplasmic male‐sterilities in the gynodioecious Thymus vulgaris. Clonal replicates of five female plants bearing at least four different male‐sterilities and originating from five different populations (i.e., five cytogenotypes), were reciprocally transplanted into the original populations. Following open pollination at each site, seeds were harvested, germinated and grown to flowering. The frequency of hermaphrodites in each progeny was used to estimate the frequency of restoration factors in each population.For all cytogenotypes, there was marked variation in the rate of restoration among populations, indicating that spatial structure in nuclear restoration factors may influence the variation in female frequency in T. vulgaris. For three out of the five cytogenotypes, higher restoration rates were obtained for females introduced into their original population, which agrees with the theoretical prediction, under some hypotheses, that restoration factors are selected for in populations that contain the associated male‐sterility (Gouyon and Couvet, 1985). The very low restoration rate in the progeny of one female clone in three populations indicates that stochastic events, such as founder effects, could lead to a local absence of restoration factors and thus the high female frequency (up to 90%) observed in some populations. Differences among cytogenotypes suggest that some male‐sterilities are associated with rarer restoration factors and are thus more likely to cause patches of females to develop in young populations.
Detection of Cross‐Reactive Antigens between Phytophthora infestans (Mont.) de Bary and Solanum Species by Indirect Enzyme‐Linked Immunosorbent Assay
Authors: Alba A. P. C. , DeVay J. E. 
Published: November 2010

AbstractCross‐reactive antigens were detected in crude preparations and in purified preparations from mycelia of Phytophthora infestans Race 4. and Race 1.2.3.4.7. with antisera for potatoes cv. King Edward and cv. Pentland Dell by using an indirect ELISA technique. Results suggest that the fungal mycelia do not easily release cross‐reactive antigens into synthetic media where they grow; that most of P. infestans cross‐reactive antigens are thermolabile and that they can be concentrated by precipitation in the presence of 40 % saturated ammonium sulphate (SAS). The results also reveal an antigenic disparity when 40 % SAS from P. infestans Race 4. mycelial preparation was assayed with antisera for King Edward and Pentland Dell. The occurrence of cross‐reactive antigens in P. infestans mycelium and their involvement in such interaction are discussed.ZusammenfassungKreuzreaktive Antigene wurden in unbearbeiteten Präparaten und in gereinigten Präparaten aus Myzel von Phytophthora infestans Rasse 4. und 1.2.3.4.7. mit Antiseren für Kartoffeln cv. King Edward und cv. Pentland Dell gefunden, indem eine indirekte ELISA‐Technik benutzt wurde. Die Ergebnisse lassen vermuten, daß die pilzlichen Myzelien die kreuzreaktiven Antigene nicht einfach in die synthetischen Medien, in denen sie wachsen, abgeben, daß die meisten der P. infestans kreuzreaktiven Antigene thermostabil sind und daß sie konzentriert werden können durch Präzipitation in 40 % gesättigtem Ammoniumsulfat (SAS). Die Ergebnisse zeigten außerdem eine Antigendisparität, wenn 40 % SAS aus einem Myzelpräparat von P. infestans Rasse 4. mit Antiseren für King Edward und Pentland Dell getestet wurde. Das Auftreten kreuzreaktiver Antigene im Myzel von P. infestans und ihre Rolle in solcher Interaktion wurde diskutiert.
The Role of Macrophomina phaseolina in Mortality of Pine Seedlings in Forest Nurseries
Authors: Reuveni R. , Madar Z. 
Published: November 2010

AbstractMortality of pine seedlings has been recently become widespread in forest nurseries. Disease symptoms are yellowing of the needles, followed by wilting and death of the seedlings. The fungus Macrophomina phaseolina (Tassi) Goid. was isolated from roots of diseased seedlings of Pinus sp. seedlings (40—75 %). The incidence of M. phaseolina in roots was correlated with the percentage of dead pine seedling in various forest nurseries. Other soil fungi isolated from infected roots are Fusarium spp. Growth chamber inoculation demonstrated the pathogenicity of the M. phaseolina isolate, while the Fusarium spp. isolates showed a low level of pathogenicity.The effect of suboptimal conditions, such as water and temperature stress with regard to M. phaseolina pathogenicity, are discussed.ZusammenfassungBei einem in Ausbreitung begriffenen Keimlingssterben verschiedener Föhrenarten (Symptome: Vergilben der Nadeln, Welken und Tod der Pflänzchen) wurde aus kranken Pflanzen häufig (40—75 %) Macrophomina phaseolina isoliert. In verschiedenen Forstgärten war das Auftreten des Pilzes mit dem Anteil abgestorbener Sämlinge korreliert. Die Pathogenität des Pilzes wurde in Klimakammern bestätigt. Fusarium‐Arten wurden aus kranken Pflanzen ebenfalls isoliert, erwiesen sich jedoch als wenig pathogen. Die Bedeutung suboptimaler Bedingungen (Wasser‐ und Temperaturstreβ) für die Pathogenität von M. phaseolina werden diskutiert.