bluuebunny/crossref_metadata_2025_split huggingface dataset:
Prehospital Surgical Cricothyrotomy in a Ground-Based 9-1-1 EMS System: A Retrospective Review
Authors: Lulla Al , Dickson Robert , Wells Michael , Gilbert Matthew , Rogers Keene Kelly , Patrick Casey 
Published: April 2024

AbstractBackground:Airway management is a cornerstone in the prehospital care of critically ill or injured patients. Surgical cricothyrotomy offers a rapid and effective solution when oxygenation and ventilation fail using less-invasive techniques. However, the exact indications, incidence, and success of prehospital surgical cricothyrotomy are unknown, with variable rates reported in the literature. This study aimed to examine prehospital indications and success rates for surgical cricothyrotomy within a large, suburban, ground-based Emergency Medical Services (EMS) system.Methods:This is a retrospective analysis of 31 patients who underwent paramedic performed surgical cricothyrotomy from 2012 through 2022. Key demographic parameters were analyzed, including the incidence of cardiac arrest, call type (trauma versus medical), initial airway management attempts, number of endotracheal intubation (ETI) attempts before surgical airway, and average time to the establishment of a surgical airway in relation to the number of ETI attempts. Surgical cricothyrotomy success was defined as the acquisition of four-phase end-tidal capnography reading. The primary data sources were the EMS electronic medical records, and descriptive statistics were calculated.Results:A total of 31 patients were included in the final analysis. Of those who received a surgical cricothyrotomy, 42% (13/31) occurred in the trauma setting, while 58% (18/31) were medical calls. In all patients who underwent surgical cricothyrotomy, the median (IQR) time to the procedure was 17 minutes (IQR = 11-24). In trauma patients, the median time to surgical cricothyrotomy was 12 minutes (IQR = 9-19) versus 19 minutes (IQR = 14-33) in medical patients. End-tidal carbon dioxide (ETCO2) detection and placement success was confirmed in 94% (29/31) of patients. Endotracheal intubation was attempted in 55% (17/31) before subsequent surgical cricothyrotomy, with 29% (9/31) receiving more than one ETI attempt. The median time to surgical cricothyrotomy when multiple prior intubation attempts occurred was 33 minutes (IQR = 23-36) compared to 14.5 minutes (IQR = 6-19) in patients without a preceding intubation attempt.Conclusion:Prehospital surgical airway can be performed by paramedics with a high degree of success. Identification of the need for surgical cricothyrotomy should be determined as soon as possible to allow for rapid securement of the airway and to ensure adequate oxygenation and ventilation.
Global Analysis of Surface Ocean CO2 Fugacity and Air‐Sea Fluxes With Low Latency
Authors: Chau Thi‐Tuyet‐Trang , Chevallier Frédéric , Gehlen Marion 
Published: April 2024

AbstractThe Surface Ocean CO2 Atlas (SOCAT) of CO2 fugacity (fCO2) observations is a key resource supporting annual assessments of CO2 uptake by the ocean and its side effects on the marine ecosystem. SOCAT data are usually released with a lag of up to 1.5 years which hampers timely quantification of recent variations of carbon fluxes between the Earth System components, not only with the ocean. This study uses a statistical ensemble approach to analyze fCO2 with a latency of one month only based on the previous SOCAT release and a series of predictors. Results indicate a modest degradation in a retrospective prediction test for 2021–2022. The generated fCO2 and fluxes for January–August 2023 show a progressive reduction in the Equatorial Pacific source following the La Niña retreat. A breaking‐record decrease in the northeastern Atlantic CO2 sink has been diagnosed on account of the marine heatwave event in June 2023.
Modeling Ion Conic Formation in Io's Auroral Footprint
Authors: Glocer A. , Garcia‐Sage K. , Sulaiman A. , Clark G. , Szalay J. R. , Sarkango Y. , Bell J. 
Published: April 2024

AbstractEnergetic ions outflowing from Jupiter's atmosphere was observed during Juno's 12th perijove crossing (PJ12) in the vicinity of Io's auroral footprint and reported by prior studies. It was hypothesized that Wave‐Particle Interactions (WPI) with ion cyclotron waves observed coincident with the ion outflow may be responsible for the heating and subsequent outflow. This study uses numerical simulation and data model comparison to test whether ion cyclotron resonant heating is indeed a plausible mechanism to explain the intense ion outflow observed. Our simulations assume that the wave heating is of limited duration due to Io's footprint motion. The simulations are moreover compared to the previously published Jupiter Energetic Particle Detector Instruments (JEDI) observations at high energies, and the lower energy Jovian Auroral Distributions Experiment (JADE) observations that were not previously reported. We find that the ion cyclotron resonant heating mechanism can indeed lead to ion conic formation and strong vertical transport under certain assumptions about the distribution of wave power with altitude. We also find that the ion outflow is energized quickly with very rapid formation of the ion conic distribution. The implications of the intense ion outflow are also examined and it is found that such strong wave heating can lead to a depletion of the topside ionosphere.
Transcriptomic and cellular decoding of scaffolds-induced suture mesenchyme regeneration
Authors: Wu Jiayi , Li Feifei , Yu Peng , Yu Changhao , Han Chuyi , Wang Yitian , Yu Fanyuan , Ye Ling 
Published: April 2024

AbstractPrecise orchestration of cell fate determination underlies the success of scaffold-based skeletal regeneration. Despite extensive studies on mineralized parenchymal tissue rebuilding, regenerating and maintaining undifferentiated mesenchyme within calvarial bone remain very challenging with limited advances yet. Current knowledge has evidenced the indispensability of rebuilding suture mesenchymal stem cell niches to avoid severe brain or even systematic damage. But to date, the absence of promising therapeutic biomaterials/scaffolds remains. The reason lies in the shortage of fundamental knowledge and methodological evidence to understand the cellular fate regulations of scaffolds. To address these issues, in this study, we systematically investigated the cellular fate determinations and transcriptomic mechanisms by distinct types of commonly used calvarial scaffolds. Our data elucidated the natural processes without scaffold transplantation and demonstrated how different scaffolds altered in vivo cellular responses. A feasible scaffold, polylactic acid electrospinning membrane (PLA), was next identified to precisely control mesenchymal ingrowth and self-renewal to rebuild non-osteogenic suture-like tissue at the defect center, meanwhile supporting proper osteointegration with defect bony edges. Especially, transcriptome analysis and cellular mechanisms underlying the well-orchestrated cell fate determination of PLA were deciphered. This study for the first time cellularly decoded the fate regulations of scaffolds in suture-bony composite defect healing, offering clinicians potential choices for regenerating such complicated injuries.
Nicotinamide Mononucleotide improves oocyte maturation of mice with type 1 diabetes
Authors: Guo Fucheng , Wang Luyao , Chen Yurong , Zhu Haibo , Dai Xiangpeng , Zhang Xiaoling 
Published: April 2024

Abstract
Background
The number of patients with type 1 diabetes rises rapidly around the world in recent years. Maternal diabetes has a detrimental effect on reproductive outcomes due to decreased oocyte quality. However, the strategies to improve the oocyte quality and artificial reproductive technology (ART) efficiency of infertile females suffering from diabetes have not been fully studied. In this study, we aimed to examine the effects of nicotinamide mononucleotide (NMN) on oocyte maturation of mouse with type 1 diabetes mouse and explore the underlying mechanisms of NMN’s effect.

Methods
Streptozotocin (STZ) was used to establish the mouse models with type 1 diabetes. The successful establishment of the models was confirmed by the results of body weight test, fasting blood glucose test and haematoxylin and eosin (H&E) staining. The in vitro maturation (IVM) rate of oocytes from diabetic mice was examined. Immunofluorescence staining (IF) was performed to examine the reactive oxygen species (ROS) level, spindle/chromosome structure, mitochondrial function, actin dynamics, DNA damage and histone modification of oocytes, which are potential factors affecting the oocyte quality. The quantitative reverse transcription PCR (RT-qPCR) was used to detect the mRNA levels of Sod1, Opa1, Mfn2, Drp1, Sirt1 and Sirt3 in oocytes.

Results
The NMN supplementation increased the oocyte maturation rate of the mice with diabetes. Furthermore, NMN supplementation improved the oocyte quality by rescuing the actin dynamics, reversing meiotic defects, improving the mitochondrial function, reducing ROS level, suppressing DNA damage and restoring changes in histone modifications of oocytes collected from the mice with diabetes.

Conclusion
NMN could improve the maturation rate and quality of oocytes in STZ-induced diabetic mice, which provides a significant clue for the treatment of infertility of the patients with diabetes.
GATA4 regulates the transcription of MMP9 to suppress the invasion and migration of breast cancer cells via HDAC1-mediated p65 deacetylation
Authors: Yang Yuxi , Song Shuangshuang , Li Shujing , Kang Jie , Li Yulin , Zhao Nannan , Ye Dongman , Qin Fengying , Du Yixin , Sun Jing , Yu Tao , Wu Huijian 
Published: April 2024

AbstractGATA-binding protein 4 (GATA4) is recognized for its significant roles in embryogenesis and various cancers. Through bioinformatics and clinical data, it appears that GATA4 plays a role in breast cancer development. Yet, the specific roles and mechanisms of GATA4 in breast cancer progression remain elusive. In this study, we identify GATA4 as a tumor suppressor in the invasion and migration of breast cancer. Functionally, GATA4 significantly reduces the transcription of MMP9. On a mechanistic level, GATA4 diminishes MMP9 transcription by interacting with p65 at the NF-κB binding site on the MMP9 promoter. Additionally, GATA4 promotes the recruitment of HDAC1, amplifying the bond between p65 and HDAC1. This leads to decreased acetylation of p65, thus inhibiting p65’s transcriptional activity on the MMP9 promoter. Moreover, GATA4 hampers the metastasis of breast cancer in vivo mouse model. In summary, our research unveils a novel mechanism wherein GATA4 curtails breast cancer cell metastasis by downregulating MMP9 expression, suggesting a potential therapeutic avenue for breast cancer metastasis.
Claudin-7 is essential for the maintenance of colonic stem cell homoeostasis via the modulation of Wnt/Notch signalling
Authors: Wang Kun , Liu Yin , Li Huimin , Liang Xiaoqing , Hao Mengdi , Yuan Dajin , Ding Lei 
Published: April 2024

AbstractIntestinal stem cells (ISCs) play a crucial role in the continuous self-renewal and recovery of the intestinal epithelium. In previous studies, we have revealed that the specific absence of Claudin-7 (Cldn-7) in intestinal epithelial cells (IECs) can lead to the development of spontaneous colitis. However, the mechanisms by which Cldn-7 maintains homeostasis in the colonic epithelium remain unclear. Therefore, in the present study, we used IEC- and ISC-specific Cldn-7 knockout mice to investigate the regulatory effects of Cldn-7 on colonic Lgr5+ stem cells in the mediation of colonic epithelial injury and repair under physiological and inflammatory conditions. Notably, our findings reveal that Cldn-7 deletion disrupts the self-renewal and differentiation of colonic stem cells alongside the formation of colonic organoids in vitro. Additionally, these Cldn-7 knockout models exhibited heightened susceptibility to experimental colitis, limited epithelial repair and regeneration, and increased differentiation toward the secretory lineage. Mechanistically, we also established that Cldn-7 facilitates the proliferation, differentiation, and organoid formation of Lgr5+ stem cells through the maintenance of Wnt and Notch signalling pathways in the colonic epithelium. Overall, our study provides new insights into the maintenance of ISC function and colonic epithelial homoeostasis.
CMYC-initiated HNF1A-AS1 overexpression maintains the stemness of gastric cancer cells
Authors: Zhao Ruinan , Guo Xiangyu , Zhang Guohao , Liu Sen , Ma Ranran , Wang Mengqi , Chen Shiming , Zhu Wenjie , Liu Yuan , Gao Peng , Liu Haiting 
Published: April 2024

AbstractCancer stem cells (CSCs) are believed to be responsible for cancer metastasis and recurrence due to their self-renewal ability and resistance to treatment. However, the mechanisms that regulate the stemness of CSCs remain poorly understood. Recently, evidence has emerged suggesting that long non-coding RNAs (lncRNAs) play a crucial role in regulating cancer cell function in different types of malignancies, including gastric cancer (GC). However, the specific means by which lncRNAs regulate the function of gastric cancer stem cells (GCSCs) are yet to be fully understood. In this study, we investigated a lncRNA known as HNF1A-AS1, which is highly expressed in GCSC s and serves as a critical regulator of GCSC stemness and tumorigenesis. Our experiments, both in vitro and in vivo, demonstrated that HNF1A-AS1 maintained the stemness of GC cells. Further analysis revealed that HNF1A-AS1, transcriptionally activated by CMYC, functioned as a competing endogenous RNA by binding to miR-150-5p to upregulate β-catenin expression. This in turn facilitated the entry of β-catenin into the nucleus to activate the Wnt/β-catenin pathway and promote CMYC expression, thereby forming a positive feedback loop that sustained the stemness of GCSCs. We also found that blocking the Wnt/β-catenin pathway effectively inhibited the function of HNF1A-AS1, ultimately resulting in the inhibition of GCSC stemness. Taken together, our results demonstrated that HNF1A-AS1 is a regulator of the stemness of GCSCs and could serve as a potential marker for targeted GC therapy.
Lineage specific transcription factor waves reprogram neuroblastoma from self-renewal to differentiation
Authors: Banerjee Deblina , Bagchi Sukriti , Liu Zhihui , Chou Hsien-Chao , Xu Man , Sun Ming , Aloisi Sara , Vaksman Zalman , Diskin Sharon J. , Zimmerman Mark , Khan Javed , Gryder Berkley , Thiele Carol J. 
Published: April 2024

AbstractTemporal regulation of super-enhancer (SE) driven transcription factors (TFs) underlies normal developmental programs. Neuroblastoma (NB) arises from an inability of sympathoadrenal progenitors to exit a self-renewal program and terminally differentiate. To identify SEs driving TF regulators, we use all-trans retinoic acid (ATRA) to induce NB growth arrest and differentiation. Time-course H3K27ac ChIP-seq and RNA-seq reveal ATRA coordinated SE waves. SEs that decrease with ATRA link to stem cell development (MYCN, GATA3, SOX11). CRISPR-Cas9 and siRNA verify SOX11 dependency, in vitro and in vivo. Silencing the SOX11 SE using dCAS9-KRAB decreases SOX11 mRNA and inhibits cell growth. Other TFs activate in sequential waves at 2, 4 and 8 days of ATRA treatment that regulate neural development (GATA2 and SOX4). Silencing the gained SOX4 SE using dCAS9-KRAB decreases SOX4 expression and attenuates ATRA-induced differentiation genes. Our study identifies oncogenic lineage drivers of NB self-renewal and TFs critical for implementing a differentiation program.
A genomic basis of vocal rhythm in birds
Authors: Sebastianelli Matteo , Lukhele Sifiso M. , Secomandi Simona , de Souza Stacey G. , Haase Bettina , Moysi Michaella , Nikiforou Christos , Hutfluss Alexander , Mountcastle Jacquelyn , Balacco Jennifer , Pelan Sarah , Chow William , Fedrigo Olivier , Downs Colleen T. , Monadjem Ara , Dingemanse Niels J. , Jarvis Erich D. , Brelsford Alan , vonHoldt Bridgett M. , Kirschel Alexander N. G. 
Published: April 2024

AbstractVocal rhythm plays a fundamental role in sexual selection and species recognition in birds, but little is known of its genetic basis due to the confounding effect of vocal learning in model systems. Uncovering its genetic basis could facilitate identifying genes potentially important in speciation. Here we investigate the genomic underpinnings of rhythm in vocal non-learningPogoniulustinkerbirds using 135 individual whole genomes distributed across a southern African hybrid zone. We find rhythm speed is associated with two genes that are also known to affect human speech, Neurexin-1 and Coenzyme Q8A. Models leveraging ancestry reveal these candidate loci also impact rhythmic stability, a trait linked with motor performance which is an indicator of quality. Character displacement in rhythmic stability suggests possible reinforcement against hybridization, supported by evidence of asymmetric assortative mating in the species producing faster, more stable rhythms. Because rhythm is omnipresent in animal communication, candidate genes identified here may shape vocal rhythm across birds and other vertebrates.
Measuring statistics-induced entanglement entropy with a Hong–Ou–Mandel interferometer
Authors: Zhang Gu , Hong Changki , Alkalay Tomer , Umansky Vladimir , Heiblum Moty , Gornyi Igor , Gefen Yuval 
Published: April 2024

AbstractDespite its ubiquity in quantum computation and quantum information, a universally applicable definition of quantum entanglement remains elusive. The challenge is further accentuated when entanglement is associated with other key themes, e.g., quantum interference and quantum statistics. Here, we introduce two novel motifs that characterize the interplay of entanglement and quantum statistics: an ‘entanglement pointer’ and a ‘statistics-induced entanglement entropy’. The two provide a quantitative description of the statistics-induced entanglement: (i) they are finite only in the presence of quantum entanglement underlined by quantum statistics and (ii) their explicit form depends on the quantum statistics of the particles (e.g., fermions, bosons, and anyons). We have experimentally implemented these ideas by employing an electronic Hong–Ou–Mandel interferometer fed by two highly diluted electron beams in an integer quantum Hall platform. Performing measurements of auto-correlation and cross-correlation of current fluctuations of the scattered beams (following ‘collisions’), we quantify the statistics-induced entanglement by experimentally accessing the entanglement pointer and the statistics-induced entanglement entropy. Our theoretical and experimental approaches pave the way to study entanglement in various correlated platforms, e.g., those involving anyonic Abelian and non-Abelian states.
Ocean cavity regime shift reversed West Antarctic grounding line retreat in the late Holocene
Authors: Lowry Daniel P. , Han Holly K. , Golledge Nicholas R. , Gomez Natalya , Johnson Katelyn M. , McKay Robert M. 
Published: April 2024

AbstractRecent geologic and modeled evidence suggests that the grounding line of the Siple Coast of the West Antarctic Ice Sheet (WAIS) retreated hundreds of kilometers beyond its present position in the middle to late Holocene and readvanced within the past 1.7 ka. This grounding line reversal has been attributed to both changing rates of isostatic rebound and regional climate change. Here, we test these two hypotheses using a proxy-informed ensemble of ice sheet model simulations with varying ocean thermal forcing, global glacioisostatic adjustment (GIA) model simulations, and coupled ice sheet-GIA simulations that consider the interactions between these processes. Our results indicate that a warm to cold ocean cavity regime shift is the most likely cause of this grounding line reversal, but that GIA influences the rate of ice sheet response to oceanic changes. This implies that the grounding line here is sensitive to future changes in sub-ice shelf ocean circulation.
Boosting thermoelectric performance of single-walled carbon nanotubes-based films through rational triple treatments
Authors: Liu Yuan-Meng , Shi Xiao-Lei , Wu Ting , Wu Hao , Mao Yuanqing , Cao Tianyi , Wang De-Zhuang , Liu Wei-Di , Li Meng , Liu Qingfeng , Chen Zhi-Gang 
Published: April 2024

AbstractSingle-walled carbon nanotubes (SWCNTs)-based thermoelectric materials, valued for their flexibility, lightweight, and cost-effectiveness, show promise for wearable thermoelectric devices. However, their thermoelectric performance requires significant enhancement for practical applications. To achieve this goal, in this work, we introduce rational “triple treatments” to improve the overall performance of flexible SWCNT-based films, achieving a high power factor of 20.29 µW cm−1 K−2 at room temperature. Ultrasonic dispersion enhances the conductivity, NaBH4 treatment reduces defects and enhances the Seebeck coefficient, and cold pressing significantly densifies the SWCNT films while preserving the high Seebeck coefficient. Also, bending tests confirm structural stability and exceptional flexibility, and a six-legged flexible device demonstrates a maximum power density of 2996 μW cm−2 at a 40 K temperature difference, showing great application potential. This advancement positions SWCNT films as promising flexible thermoelectric materials, providing insights into high-performance carbon-based thermoelectrics.
Structural and biochemical analysis of family 92 carbohydrate-binding modules uncovers multivalent binding to β-glucans
Authors: Hao Meng-Shu , Mazurkewich Scott , Li He , Kvammen Alma , Saha Srijani , Koskela Salla , Inman Annie R. , Nakajima Masahiro , Tanaka Nobukiyo , Nakai Hiroyuki , Brändén Gisela , Bulone Vincent , Larsbrink Johan , McKee Lauren S. 
Published: April 2024

AbstractCarbohydrate-binding modules (CBMs) are non-catalytic proteins found appended to carbohydrate-active enzymes. Soil and marine bacteria secrete such enzymes to scavenge nutrition, and they often use CBMs to improve reaction rates and retention of released sugars. Here we present a structural and functional analysis of the recently established CBM family 92. All proteins analysed bind preferentially to β−1,6-glucans. This contrasts with the diversity of predicted substrates among the enzymes attached to CBM92 domains. We present crystal structures for two proteins, and confirm by mutagenesis that tryptophan residues permit ligand binding at three distinct functional binding sites on each protein. Multivalent CBM families are uncommon, so the establishment and structural characterisation of CBM92 enriches the classification database and will facilitate functional prediction in future projects. We propose that CBM92 proteins may cross-link polysaccharides in nature, and might have use in novel strategies for enzyme immobilisation.