bluuebunny/crossref_metadata_2025_split huggingface dataset:
A core outcome set for adult general ICU patients
Authors: Kjær Maj-Brit Nørregaard , Bruun Camilla Rahbek Lysholm , Granholm Anders , Møller Morten Hylander , Rasmussen Bodil Steen , Mortensen Camilla Bekker , Poulsen Lone Musaeus , Strøm Thomas , Laerkner Eva , Brøchner Anne Craveiro , Haberlandt Trine , Bunzel Anne-Marie Gellert , Herløv Louise Stenbryggen , Holm Anna , Sivapalan Praleene , Estrup Stine , Cronhjort Maria , Schandl Anna , Laake Jon Henrik , Hofsø Kristin , Blokzijl Fredrike , Keus Frederic , Pfortmueller Carmen Andrea , Ostermann Marlies , Cole Jade M , Wise Matt P , Szczeklik Wojciech , Wludarczyk Anna , Jovaiša Tomas , Cecconi Maurizio , Sigurdsson Martin Ingi , Nalos Marek , Hästbacka Johanna , Mäkinen Marja , Hammond Naomi , Litton Edward , Haines Kimberley , Myatra Sheila Nainan , Tirupakuzhi Vijayaraghavan Bharath Kumar , Yadav Kavita , Jha Vivekanand , Venkatesh Balasubramanian , Egerod Ingrid , Perner Anders , Collet Marie O 
Published: May 2024

AbstractPurposeRandomised clinical trials should ideally use harmonised outcomes that are important to patients and to facilitate meta-analyses and ensuring generalisability. Core outcome sets for specific subsets of ICU patients exist, e.g., respiratory failure, delirium, and COVID-19, but not for ICU patients in general. Accordingly, we aimed to develop a core outcome set for adult general ICU patients.MethodsWe developed a core outcome set in Denmark following the Core Outcome Measures in Effectiveness Trials (COMET) Handbook. We used a modified Delphi consensus process with multiple methods design, including literature review, survey, semi-structured interviews, and discussions with initially five Danish research panels, involving adult ICU survivors, family members, clinicians, and researchers. The core outcome set was internationally validated in local panels in 14 countries and revised accordingly.ResultsWe identified 329 published outcomes, of which 50 were included in the 264 participant Delphi survey. After 82 semi-structured survey participant interviews no additional outcomes were added. The first survey round was completed by 249 (94%) participants, and 202 (82%) contributed to the final third round. The initial core outcome set comprised six core outcomes. International validation involved 217 research panel members and resulted in the final core outcome set of survival, free of life support, free of delirium, out of hospital, health-related quality of life, and cognitive function.ConclusionsWe developed and internationally validated a core outcome set with six core outcomes to be used in research, specifically clinical trials involving adult general ICU patients.
Formate from THF-C1 metabolism induces the AOX1 promoter in formate dehydrogenase-deficientPichia pastoris
Authors: Bustos Cristina , Berrios Julio , Fickers Patrick 
Published: May 2024

AbstractInPichia pastoris(Komagataella phaffii), formate is a recognized alternative inducer to methanol for expression systems based on the AOX1 promoter (pAOX1). By disrupting the formate dehydrogenase encodingFDH1gene, we converted such a system into a self-induced one, as adding any inducer in the culture medium is no longer requested for pAOX1 induction. In cells, formate is generated from serine through the THF-C1 metabolism, and it cannot be converted into carbon dioxide in anfdh1Δ strain. Under non-repressive culture conditions, such as on sorbitol, the intracellular formate generated from the THF-C1 metabolism is sufficient to induce pAOX1 and initiate protein synthesis. This was evidenced for two model proteins, namely intracellular eGFP and secreted CalB lipase fromC. antarctica. Similar protein productivities were obtained for anfdh1Δ strain on sorbitol and a non-disrupted strain on sorbitol-methanol. Considering aP. pastoris fdh1Δstrain as a workhorse for recombinant protein synthesis paves the way for the further development of methanol-free processes inP. pastoris.
Evaluating apoptotic gene efficiency for CHO culture performance using targeted integration
Authors: Catalán-Tatjer David , Ganesan Saravana Kumar , Martínez-Monge Iván , Grav Lise M. , Lavado-García Jesús , Nielsen Lars K. 
Published: May 2024

ABSTRACTChinese hamster ovary (CHO) cells have long been the favoured platform for producing complex biopharmaceuticals such as monoclonal antibodies (mAbs). Cell death is a critical factor in all CHO cultures, dictating duration until harvest in batch cultures and viable cell density in perfusion. The programmed cell death, or apoptosis, pathway has been widely studied due to its relevance in affecting cell culture performance and the extensive knowledge about its protein-to-protein interaction network. However, clonal variation seen with random integration has confounded results and it remains unclear which effector genes should be overexpressed. Here, we employed the recombinase-mediated cassette exchange (RMCE) strategy to develop isogenic cell lines expressing one copy of erythropoietin, as model protein product, and various anti-apoptotic genes: bcl-2 from CHO and human origin, bcl-xL from CHO and human origin, mcl-1 and bhrf-1. We tested the generated isogenic cell lines in the presence of sodium butyrate, a well-known apoptotic initiator, in batch culture. The most promising candidates were cultured in fed-batch in the microbioreactor ambr®15 system. The observed phenotype varied significantly depending on the overexpressed gene, therefore the metabolic differences were further characterized using multiplexed quantitative proteomics. We showed that overexpressing bcl-2 from CHO origin significantly improved productivity and established a methodology to successfully test candidate genes via targeted integration. This will enable future metabolic engineering strategies to be more comparable and overcome the challenges faced thus far.
Quantitative imaging of loop extruders rebuilding interphase genome architecture after mitosis
Authors: Brunner Andreas , Morero Natalia Rosalia , Zhang Wanlu , Hossain M. Julius , Lampe Marko , Pflaumer Hannah , Halavatyi Aliaksandr , Peters Jan-Michael , Beckwith Kai S. , Ellenberg Jan 
Published: May 2024

SummaryHow cells establish the interphase genome organization after mitosis is incompletely understood. Using quantitative and super-resolution microscopy, we show that the transition from a Condensin to a Cohesin-based genome organization occurs dynamically over two hours. While a significant fraction of Condensins remains chromatin-bound until early Gl, Cohesin-STAGl and its boundary factor CTCF are rapidly imported into daughter nuclei in telophase, immediately bind chromosomes as individual complexes and are sufficient to build the first interphase TAD structures. By contrast, the more abundant Cohesin-STAG2 accumulates on chromosomes only gradually later in Gl, is responsible for compaction inside TAD structures and forms paired complexes upon completed nuclear import. 0ur quantitative time-resolved mapping of mitotic and interphase loop extruders in single cells reveals that the nested loop architecture formed by sequential action of two Condensins in mitosis is seamlessly replaced by a less compact, but conceptually similar hierarchically nested loop architecture driven by sequential action of two Cohesins.
Critical role of Gα12 and Gα13 proteins in TGF-β-induced myofibroblast differentiation
Authors: Reed Eleanor B. , Sitikov Albert , Hamanaka Robert B. , Cetin-Atalay Rengül , Mutlu Gökhan M. , Mongin Alexander A. , Dulin Nickolai O. 
Published: May 2024

AbstractMyofibroblast differentiation, characterized by accumulation of cytoskeletal and extracellular matrix proteins by fibroblasts, is a key process in wound healing and pathogenesis of tissue fibrosis. Transforming growth factor-β (TGF-β) is the most powerful known driver of myofibroblast differentiation. TGF-β signals through transmembrane receptor serine/threonine kinases that phosphorylate Smad transcription factors (Smad2/3) leading to activation of transcription of target genes. Heterotrimeric G proteins mediate a distinct signaling from seven-transmembrane G protein coupled receptors, not commonly linked to Smad activation. We asked if G protein signaling plays any role in TGF-β-induced myofibroblast differentiation, using primary cultured human lung fibroblasts. Activation of Gαs by cholera toxin blocked TGF-β-induced myofibroblast differentiation without affecting Smad2/3 phosphorylation. Inhibition of Gαi by pertussis toxin, or siRNA-mediated combined knockdown of Gαq and Gα11 had no significant effect on TGF-β-induced myofibroblast differentiation. A combined knockdown of Gα12 and Gα13 resulted in a drastic inhibition of TGF-β-stimulated expression of myofibroblast marker proteins (collagen-1, fibronectin, smooth-muscle α-actin), with siGα12 being significantly more potent than siGα13. Mechanistically, a combined knockdown of Gα12 and Gα13 resulted in a substantially reduced phosphorylation of Smad2 and Smad3 in response to TGF-β, which was accompanied by a significant decrease in the expression of TGFβ receptors (TGFBR1, TGFBR2) and of Smad3 under siGα12/13 conditions. In conclusion, our study uncovers a novel role of Gα12/13 proteins in the control of TGF-β signaling and myofibroblast differentiation.
Extending CityGML with public input – social ADE
Authors: Zarei Farzaneh , Nik-Bakht Mazdak 
Published: May 2024

PurposeThis paper aims to enrich the 3D urban models with data contributed by citizens to support data-driven decision-making in urban infrastructure projects. We introduced a new application domain extension to CityGML (social – input ADE) to enable citizens to store, classify and exchange comments generated by citizens regarding infrastructure elements. The main goal of social – input ADE is to add citizens’ feedback as semantic objects to the CityGML model.Design/methodology/approachFirstly, we identified the key functionalities of the suggested ADE and how to integrate them with existing 3D urban models. Next, we developed a high-level conceptual design outlining the main components and interactions within the social-input ADE. Then we proposed a package diagram for the social – input ADE to illustrate the organization of model elements and their dependencies. We also provide a detailed discussion of the functionality of different modules in the social-input ADE.FindingsAs a result of this research, it has seen that informative streams of information are generated via mining the stored data. The proposed ADE links the information of the built environment to the knowledge of end-users and enables an endless number of socially driven innovative solutions.Originality/valueThis work aims to provide a digital platform for aggregating, organizing and filtering the distributed end-users’ inputs and integrating them within the city’s digital twins to enhance city models. To create a data standard for integrating attributes of city physical elements and end-users’ social information and inputs in the same digital ecosystem, the open data model CityGML has been used.
Can corporate ESG investing boost zombie firms back to normal? Evidence from Chinese firms
Authors: Ren Guangqian , Li Junchao , Zhao Mengjie , Zheng Minna 
Published: May 2024

Purpose
This study aims to examine the ramifications of corporate environmental, social and governance (ESG) investing in zombie firms and considers how external funding support may moderate this relationship given the sustainable nature of ESG performance, which often incurs costs.

Design/methodology/approach
Panel regression analyses used data from China’s A-share listed companies from 2011 to 2019, resulting in a data set comprising 6,054 observations.

Findings
Despite firms’ additional financial burdens, corporate ESG investing emerges as a catalyst in resurrecting zombie firms by attracting investor attention. Further analysis underscores the significance of funding support from entities such as the government and banks in alleviating ESG cost pressures and enhancing the efficacy of corporate ESG investing. Notably, the positive impact of corporate ESG investing is most pronounced in non-heavily polluting and non-state-owned firms. The results of classification tests reveal that social (S) and governance (G) investing yield greater efficacy in revitalizing zombie firms compared to environmental (E) investing.

Practical implications
This research enriches the discourse on corporate ESG investing and offers insights for governing zombie firms and shaping government policies.

Originality/value
By extending the domain of ESG research to encompass zombie firms, this paper sheds light on the multifaceted role of corporate ESG investing. Furthermore, this study comprehensively evaluates the influence of external funding support on the positive outcomes of ESG investing, thereby contributing to the resolution of the longstanding debate on the relationship between ESG performance and corporate financial performance, particularly with regard to ESG costs and benefits.
CompEntre 4.0: developing and validating a competency model for Industry 4.0 startups
Authors: Wahl Dario , Münch Jürgen 
Published: May 2024

PurposeCompetency models are widespread in entrepreneurship and help develop educational offerings. Although existing models cater to specific sub-disciplines, the field of Industry 4.0 startups still needs a tailored competency. Therefore, this study aims to bridge this gap by developing a specific competency model to address the unique challenges in Industry 4.0 entrepreneurship.Design/methodology/approachThe research approach involved a content analysis and interview study in compiling and categorizing the necessary competencies to succeed in the Industry 4.0 domain. The developed model was subjected to different forms of validation using the Content Validity Index and inter-rater reliability incorporating expert feedback.FindingsThe described multi-methodological approach resulted in the proposed “CompEntre 4.0” model, which contains 23 crucial competencies for Industry 4.0 startups. The results of this model validation demonstrate that it meets the necessary threshold values, establishing its reliability and potential for future use and further improvement.Practical implicationsBy providing a structured framework tailored to the specific demands of this domain, the competency model has the potential to guide and empower entrepreneurs, improving their prospects for success in the rapidly evolving landscape of Industry 4.0.Originality/valueWhile there are specific competency models for the entrepreneurship field and for specific sub-disciplines of entrepreneurship, there is, despite numerous specifics, no competency model for Industry 4.0 entrepreneurship yet.
Examination of the effects of learning capabilities and market orientation of logistics companies on innovation and logistics performance
Authors: Adiguzel Zafer , Sonmez Cakir Fatma , Yesilot Zehir Songul , Zehir Cemal 
Published: May 2024

Purpose
In today’s economic conditions, businesses face great challenges related to competitiveness. The effort to respond flexibly to the changing business environment and customers’ demands constantly puts pressure on innovation. For this reason, research purpose to examine the effects of learning capabilities and market orientation in logistics companies during the pandemic.

Design/methodology/approach
In this research, a quantitative method was adopted and partial least squares structural equation modeling (PLS-SEM) was applied. The research is based on four variables. A conceptual research model was established through literature review and the model was tested. Within the scope of this research, A sample of 544 participants was taken to construct and test the model, and a 39-statement scale was presented to them. After presenting the reliability and validity values on the model, hypotheses were tested. Analyses were conducted using Smart-PLS 3.3 software.

Findings
As a result of the research conducted in logistics companies, the positive effects of learning orientation on performance outputs and the positive effects of market orientation as both a mediator and an independent variable show that these concepts are important for company management.

Research limitations/implications
Considering the limitations of the research, it would be wrong to generalize the results of the analysis for different sectors because the data were collected from experts working in logistics companies. Therefore, it is necessary to make evaluations taking into account the limitations of the research. In future studies, it is recommended to make comparisons with research in different sectors, taking into account the limitations, and to enrich the literature with more research in this area.

Practical implications
Because of the importance of logistics activities seen in all countries with the pandemic period, it can be explained as a result of the analyses that the performance outputs are positively affected by the learning orientation, the companies’ adaptation to continuous change and development and their emphasis on market orientation at the same time.

Originality/value
The research is an original study as it analyzes the effects of learning orientations of logistics companies on the problems and opportunities experienced in the supply and distribution channel along with the pandemic process.
Cross-validating the measurement scale for consumers’ experience with hospitality and tourism technology: a multi-sector approach
Authors: Shin Hyejo Hailey , So Kevin Kam Fung , Jeong Miyoung 
Published: May 2024

Purpose
This study aims to cross-validate the technology experience (TE) scale and examine the potential differences in consumer TE across three different sectors.

Design/methodology/approach
Across three separate studies, the TE scale and its psychometric properties and consequences were examined across three distinctive hospitality and tourism (H&T) sectors: accommodations (n = 640), food and beverage (n = 615) and tourism (n = 592).

Findings
The findings consistently show that TE is a second-order formative construct with nine dimensions. Furthermore, the factor structure of consumer TE is consistent across the focal sectors, enhancing the TE scale’s generalizability. While the dimensions consisting of TE were identical across the three sectors, the composites of TE were formed differently across the sectors, demonstrating the differences in consumers’ TE across the three sectors.

Practical implications
This research offers practical implications to the H&T industry regarding the different impacts of various TE dimensions on consumers’ overall experiences, thereby creating overall satisfaction and behavioral intentions.

Originality/value
To the best of the authors’ knowledge, this research was the first attempt to examine the differences in consumers’ TE across the sectors of the H&T industry. By identifying the different impacts of TE dimensions on consumers’ overall experience, this research provides theoretical and practical contributions by confirming the distinct characteristics of the sectors under the H&T industry.
From perception to practice: quality management in multinational company from a Swedish perspective
Authors: Wangwacharakul Promporn 
Published: May 2024

Purpose
This study explores the interplay between levels of cultures and aspects of quality management (QM), aiming to develop a conceptual framework and introduce propositions regarding managing quality in a multinational company (MNC).

Design/methodology/approach
A conceptual framework delineating the relationship between the levels of cultures in MNCs and various aspects of QM is proposed. Thereafter, based on a theory elaboration approach, a case study in Swedish facilities of MNCs is used to further illustrate the link between constructs of the framework, contributing to the identification of challenges and possibilities in managing quality in MNCs.

Findings
The research identifies key propositions regarding the intricate relationship between levels of cultures and their influences on aspects of QM in MNC. Proposition 1 emphasises the impact of national cultural differences on perceptions of QM principles. Proposition 2 reveals that diverse QM perceptions affect global consistency in QM practices. However, proposition 3 suggests that emphasising technical aspects in common QM practices fosters shared perceptions and a cohesive organisational culture, leading to Proposition 4, that a QM-centric organisational culture mediates national cultural differences, facilitating the management of quality globally.

Research limitations/implications
This research relies on a case study from a Swedish perspective. There is a need for quantitative or mixed method approaches to validate the proposed framework.

Practical implications
This research yields practical insights into cross-cultural QM challenges and possibilities in MNCs.

Originality/value
By integrating national and organisational culture into the QM framework, this research offers a conceptual model and propositions as a foundation for future cross-cultural QM research in MNCs.
The implementation of berth allocation policies that enable Just-in-Time arrival in port calls
Authors: Mubder Abd Alla Ali Mubder 
Published: May 2024

PurposeJust-in-Time (JIT) arrival in the context of port calls can be used to reduce fuel and emissions to achieve environmental targets. The purpose of this paper is to study the implementation process of the Pre-booking Berth Allocation Policy (PBP) and analyze the effectiveness of this policy for the implementation of JIT in port calls.Design/methodology/approachThe study deploys a single case study approach to empirically analyze port authority’s transition from a first-come-first-served (FCFS) arrival policy to the PBP. Observations, interviews and documents were used to collect data during 2020–2022. The analysis deployed the capability, opportunity, motivation and behavior model.FindingsThe transition from FCFS to PBP requires an inter-organizational approach, engaging external actors to manage diverse needs and preferences. This fosters effective transition and addresses conflicting interests. The PBP enables JIT arrival, enhancing operational and environmental performance, but faces barriers such as resource dependency and lack of trust. Information sharing capability among the actors, supported by Port Community Systems and adjusted operating rules, is crucial. Moreover, the PBP facilitates integration between sea and hinterland transportation, improving planning and efficiency across maritime transportation chains.Research limitations/implicationsThe single case study limits the generalizability of the findings.Practical implicationsImplementing the PBP is complex and demands careful planning from managers. Involving port call actors in the transition is helpful for port managers because they provide valuable feedback and highlight overlooked issues.Originality/valueFive propositions are suggested to highlight the role of inter-organizational collaboration, information sharing and overcoming barriers such as resource dependency to successfully realize the benefits of JIT in maritime transportation chains.