bluuebunny/crossref_metadata_2025_split huggingface dataset:
Improving fatty acid production in escherichia coli through the overexpression of malonyl coA‐Acyl carrier protein transacylase
Authors: Zhang Xiujun , Agrawal Arpita , San Ka‐Yiu 
Published: September 2011

AbstractThe microbial biosynthesis of free fatty acid, which can be used as precursors for the production of fuels or chemicals from renewable carbon sources, has attracted significant attention in recent years. Free fatty acids can be produced by introducing an acyl‐carrier protein (ACP) thioesterase (TE) gene into Escherichia coli. The first committed step of fatty acid biosynthesis is the conversion of acetyl‐CoA to malonyl‐CoA by an adenosine triphosphate (ATP)‐dependent acetyl‐CoA carboxylase followed by the conversion of malonyl‐CoA to malonyl‐ACP through the enzyme malonyl CoA‐acyl carrier protein transacylase (MCT; FabD). The E. coli fabD gene encoding MCT has been cloned and studied. However, the effect of FabD overexpression in a fatty acid overproducing strain has not been examined. In this study, we examined the effect of FabD overexpression in a fatty acid overproducing strain carrying an acyl‐ACP TE. Specifically, the effect of overexpressing a fabD gene from four different organisms on fatty acid production was compared. The strains carrying a fabD gene from E. coli, Streptomyces avermitilis MA‐4680, or Streptomyces coelicolor A3(2) improved the free fatty acid production; these three strains produced more free fatty acids, about 11% more, than the control strain. The strain carrying a fabD gene from Clostridium acetobutylicum ATCC 824, however, produced similar quantities of free fatty acids as the control strain. In addition, the three FabD overexpressed strains also have higher fatty acid/glucose yields. The results suggested that FabD overexpression can be used to improve free fatty acid production by increasing the malonyl‐ACP availability. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2012
Biotin‐assisted folding of streptavidin on the yeast surface
Authors: Hong Lim Kok , Hwang Inseong , Park Sheldon 
Published: September 2011

AbstractYeast surface display allows heterologously expressed proteins to be targeted to the exterior of the cell wall and thus has a potential as a biotechnology platform. In this study, we report the successful display of functional streptavidin on the yeast surface. Streptavidin binds the small molecule biotin with high affinity (Kd ∼10−14
M) and is used widely in applications that require stable noncovalent interaction, including immobilization of biotinylated compounds on a solid surface. As such, engineering functional streptavidin on the yeast surface may find novel uses in future biotechnology applications. Although the molecule does not require any post‐translational modification, streptavidin is difficult to fold in bacteria. We show that Saccharomyces cerevisiae can fold the protein correctly if induced at 20°C. Contrary to a previous report, coexpression of anchored and soluble streptavidin subunits is not necessary, as expressing the anchored subunit alone is sufficient to form a functional complex. For unstable monomer mutants, however, addition of free biotin during protein induction is necessary to display a functional molecule, suggesting that biotin helps the monomer fold. To show that surface displayed streptavidin can be used to immobilize other biomolecules, we used it to capture biotinylated antibody, which is then used to immunoprecipitate a protein target. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2012
Expression of biologically active human clotting factor IX in Drosophila S2 cells: γ‐carboxylation of a human vitamin K‐dependent protein by the insect enzyme
Authors: Vatandoost Jafar , Zomorodipour Alireza , Sadeghizadeh Majid , Aliyari Roghayeh , Bos Mettine H. A. , Ataei Fariba 
Published: September 2011

AbstractThe Drosophila γ‐glutamyl carboxylase (dγC) has substrate recognition properties similar to that of the vertebrate γ–carboxylase (γC), and its carboxylated product yield, in vitro, was shown to be more than that obtained with the human enzyme. However, whether the Drosophila enzyme is able to γ‐carboxylate the human vitamin K‐dependent (VKD) proteins, such as the human coagulation factor IX (hFIX), as synthesized in cultured Drosophila cells was not known. To examine this possibility, the Drosophila Schnider (S2) cell line was transfected with a metallothionein promoter‐regulated hFIX‐expressing plasmid. After induction with copper ion, expression efficiency of the active hFIX was analyzed by performing enzyme‐linked immunosorbent assey (ELISA) and coagulation test on the culture supernatant of the transfected S2 cells during 72 h of postinduction. In comparison with Chinese hamster ovary cell line, S2 cells showed higher (∼12‐fold) expression level of the hFIX. The γ‐carboxylation of the Drosophila‐derived hFIX was confirmed by evaluation of the expressed protein, after being precipitated with barium citrate. The biological activity of the S2 cell‐derived hFIX indicated the capability of S2 cells to fulfill the required γ‐carboxylation of the expressed hFIX. Coexpression of the human γ–glutamyl carboxylases (hγC) was also shown to improve both expression and γ‐carboxylation of the hFIX. This is the first in vivo data to describe the ability of the dγC to recognize the human‐based propeptide as substrate, which is an essential step for production of biologically active γ‐carboxylated VKD proteins. © 2011 American Institute of Chemical Engineers Biotechnol. Prog., 2012
A Study of White‐Spirit Vapor Combustion on Pt/Al2O3 Catalyst
Authors: Mihaela Manta C. , Bozga G. , Bercaru G. , Sorin Bildea C. 
Published: September 2011

AbstractThe objective of the work is to investigate the catalytic combustion of white spirit solvent vapors at low concentration in air, typical for depollution applications, on a commercial catalyst, Pt/γ‐Al2O3 (0.5 wt‐% Pt). To study the influence of the main variables on the process kinetics, experiments were performed under various operating conditions (temperature domain: 150–350 °C; flow rates: 125–250 mL min–1; white spirit concentrations: 220–260 ppmv). The stoichiometry and kinetics of the vapor combustion was described globally by using a pseudo‐compound, representing the average molecular weight and the carbon/hydrogen ratio of the mixture. Experimental data were reasonably well correlated by a first order rate expression with respect to the apparent concentration of the lumping pseudo‐component.
ChemInform Abstract: Large‐Scale Synthesis of Uniform Silver Orthophosphate Colloidal Nanocrystals Exhibiting High Visible Light Photocatalytic Activity.
Authors: Dinh Cao‐Thang , Nguyen Thanh‐Dinh , Kleitz Freddy , Do Trong‐On 
Published: September 2011

AbstractUniform colloidal Ag3PO4 nanocrystals are synthesized by reaction of AgNO3 with H3PO4 and oleylamine in toluene/EtOH at room temperature.
ChemInform Abstract: Hydrothermal Synthesis of [C6H16N2] [In2Se3(Se2)]: A New One‐Dimensional Indium Selenide.
Authors: Ewing Sarah J. , Powell Anthony V. , Vaqueiro Paz 
Published: September 2011

AbstractThe new title compound (III) crystallizes in the monoclinic space group C2/c with Z = 4 (single crystal XRD).
ChemInform Abstract: Ba3Y2B6O15, a Novel Cubic Borate.
Authors: Zhao Sangen , Yao Jiyong , Zhang Guochun , Fu Peizhen , Wu Yicheng 
Published: September 2011

AbstractThe title compound is prepared by solid state reaction of a stoichiometric mixture of BaCO3, Y2O3, and H3BO3 (773 K for 24 h and 1123 K for 72 h).
ChemInform Abstract: Material Design and High‐Pressure Synthesis of Novel A‐Site‐Ordered Perovskites AMn3Al4O12 (A: Y, Yb, and Dy) with Square‐Planar‐Coordinated Mn3+.
Authors: Saito Takashi , Tohyama Takenori , Woodward Patrick M. , Shimakawa Yuichi 
Published: September 2011

AbstractThe title compounds are prepared by solid state reaction of stoichiometric mixtures of Ln2O3 (Ln: Y, Dy, Yb), Al2O3, and Mn2O3 (gold capsule, 900 °C, 9 GPa, 1 h).
ChemInform Abstract: Enzymatic Kinetic Resolution of Organochalcogenides in Supercritial CO2.
Authors: Gariani Rogerio A. , Luengo Fernando A. G. , Vale Luiz A. S. , Bazito Reinaldo C. , Comasseto Joao V. 
Published: September 2011

AbstractThe process is optimized through a set of experiments (21 runs) using a Doehlert experimental design resulting in maximal e.e.
ChemInform Abstract: PtCl2‐Catalyzed Cyclization of o‐Diethynylbenzene Derivatives Triggered by Intramolecular Nucleophilic Attack.
Authors: Miki Koji , Kuge Hiroyuki , Umeda Rui , Sonoda Motohiro , Tobe Yoshito 
Published: September 2011

AbstractPt‐catalyzed cyclization of o‐dialkynylbenzene derivatives bearing a hydroxyethyl group [cf.
ChemInform Abstract: SBA‐15‐Pr—SO3H as Nanoreactor Catalyzed Oxidation of Sulfides into Sulfoxides.
Authors: Bahrami Kiumars , Khodaei Mohammad M. , Fattahpour Peyman 
Published: September 2011

AbstractMesoporous propylsulfonic acid‐functionalized SBA‐15 channels serve as effective nanoreactors for the hydrogen peroxide oxidation of aromatic and aliphatic sulfides to sulfoxides, e.g. (II) and (IV).
ChemInform Abstract: Simple Methodology for the Preparation of Amino Alcohols from Amino Acid Esters Using NaBH4‐Methanol in THF.
Authors: Goncalves R. S. B. , Pinheiro A. C. , da Silva E. T. , da Costa J. C. S. , Kaiser C. R. , de Souza M. V. N. 
Published: September 2011

AbstractThe presented procedure is simple, inexpensive, and proceeds without any racemization.
ChemInform Abstract: Diethylamine Functionalized Polyethylene Glycol as a Novel and Efficient Catalyst for Knoevenagel Condensation.
Authors: Ye Wen , Jiang Hong , Yang Xian‐Cao 
Published: September 2011

AbstractThe basic PEG catalyst enables the Knoevenagel condensation of malononitrile (II) and cyanoacetate (IV) with various aromatic aldehydes (I) under solvent‐free conditions at room temperature.
ChemInform Abstract: Deprotection Chemistry Mediated by ZrOCl2·8H2O: An Efficient, Mild, and Green Method for the Conversion of Oximes to Carbonyl Compounds in Aqueous Acetone.
Authors: Saikia Lakhinath , Das Subrata , Thakur Ashim Jyoti 
Published: September 2011

AbstractZirconium oxychloride octahydrate is less‐toxic, moisture‐stable, inexpensive and eco‐friendly mediator for the conversion of oximes to carbonyl compounds.
ChemInform Abstract: Highly Efficient Bifunctional Organocatalysts for the Asymmetric Michael Addition of Ketones to Nitroolefins.
Authors: Yu Chuanming , Qiu Jun , Zheng Fei , Zhong Weihui 
Published: September 2011

AbstractA novel proline derived chiral bis‐amine catalyst is designed and successfully applied to the title process.
ChemInform Abstract: Neighbouring Formyl Group Assisted Oxidation of o‐Alkynylarenecarbaldehydes by an Iodine/Water System.
Authors: Sakthivel Karuppusamy , Srinivasan Kannupal 
Published: September 2011

AbstractIodine‐mediated oxidation of o‐alkynylarenecarbaldehydes (I) and (III) provides an efficient access towards a variety of tricarbonyl compounds (II) and (IV), resp., in good yields.
ChemInform Abstract: An Efficient FeCl3‐Promoted O‐Alkyl Cleavage of Esters to Carboxylic Acids.
Authors: Lian Xiaoyan , Fu Shaomin , Ma Tongmei , Li Shunbin , Zeng Wei 
Published: September 2011

AbstractVarious ester substrates can be cleaved with success, whereby the ethyl esters are the most efficient ones.
ChemInform Abstract: Cobalt‐Catalyzed Coupling of Alkyl Grignard Reagent with Benzamide and 2‐Phenylpyridine Derivatives Through Directed C—H Bond Activation under Air.
Authors: Chen Quan , Ilies Laurean , Yoshikai Naohiko , Nakamura Eiichi 
Published: September 2011

AbstractVarious aromatic carboxamides and ortho‐phenylpyridine derivatives are ortho‐alkylated with Grignard reagents using air as a sole oxidant.
ChemInform Abstract: Functionalization of Mesoporous Carbon with Superbasic MgO Nanoparticles for the Efficient Synthesis of Sulfinamides.
Authors: Chakravarti Rajashree , Mano Ajayan , Iwai Hideo , Aldeyab Salem S. , Kumar R. Pradeep , Kantam M. Lakshmi , Vinu Ajayan 
Published: September 2011

AbstractNewly synthesized basic MgO‐functionalized mesoporous carbon with a large pore volume, a high surface area, and large pore diameter shows high activity in the selective synthesis of sulfinamides from arylsulfonyl chlorides.
ChemInform Abstract: trans‐4‐Hydroxy‐L‐proline: A Novel Starting Material for N‐Alkylpyrroles Synthesis.
Authors: Kumar A. Vijay , Rao K. Rama 
Published: September 2011

AbstractThe reaction of aldehydes (I) with title compound (II) is studied for the first time, resulting in the formation of N‐alkyl‐pyrroles (III) in good to high yields.
ChemInform Abstract: Regioselective One‐Pot Synthesis of 3,3′‐Diindolylethylene Derivatives and Study of Their Cytotoxic Activity.
Authors: Mandal Madhumita , Kumar Deepak , Roy Rajneeta , Sen Rupashree , Das Padma , Chatterjee Mitali , Jaisankar Parasuraman 
Published: September 2011

AbstractFriedel—Crafts‐type acylation of 2‐arylindoles (I) yields regioselectively title compounds (III) together with the expected 3‐acetyl indoles (IV).
ChemInform Abstract: Oxidative Aromatic C—N Bond Formation: Convenient Synthesis of N‐Amino‐3‐nitrile‐indoles via FeBr3‐Mediated Intramolecular Cyclization.
Authors: Zheng Zisheng , Tang Lina , Fan Yanfeng , Qi Xiuxiang , Du Yunfei , Zhang‐Negregrie Daisy 
Published: September 2011

AbstractThe title compounds are of biological interest.
ChemInform Abstract: Sequential Coupling/Desilylation—Coupling/Cyclization in a Single Pot under Pd/C—Cu Catalysis: Synthesis of 2‐(Hetero)aryl Indoles.
Authors: Rao R. Mohan , Upendar Reddy C. H. , Alinakhi Alinakhi , Mulakayala Naveen , Alvala Mallika , Arunasree M. K. , Poondra Rajamohan R. , Iqbal Javed , Pal Manojit 
Published: September 2011

AbstractA new, simple, and efficient method to prepare 2‐(hetero)aryl indoles is reported.
ChemInform Abstract: Synthesis and Regiospecific Methylation of New Benzimidazolyl β‐Ketosulfones and β‐Hydroxysulfones.
Authors: Kumar N. D. Mahesh , Dubey P. K. 
Published: September 2011

AbstractA series of benzimidazolyl β‐ketosulfones and β‐hydroxysulfones of type (IV) and (V) are prepared.
ChemInform Abstract: Facile Synthesis of 3‐Alkyl‐5‐methyloxazolidine‐2,4‐diones (V) and N‐Lactoyl‐N,N′‐dialkylureas (VII)
Authors: Saliu Franceso , Galliani Guido , Orlandi Marco , Rindone Bruno , Tolppa Eeva‐Liisa , Suarez‐Bertoa Ricardo 
Published: September 2011

Abstractstarting from propylene carbonate (I)
ChemInform Abstract: Migratory Decarboxylative Coupling of Coumarins: Synthetic and Mechanistic Aspects.
Authors: Jana Ranjan , Partridge James J. , Tunge Jon A. 
Published: September 2011

AbstractThe allylation of coumarins with unusual regioselectivity is investigated and a simple method for γ‐allylation of coumarins based on this finding is developed.
ChemInform Abstract: Synthesis of 4‐Hydroxycoumarin Heteroarylhybrids as Potential Antimicrobial Agents.
Authors: Siddiqui Zeba N. , Musthafa Mohammed T. N. , Ahmad Anis , Khan Asad U. 
Published: September 2011

AbstractThe title compounds are obtained in the reaction of coumarin (I) with various heteroaldehydes.
ChemInform Abstract: Facile Synthesis of Spiroisoquinolines Based on Photocycloaddition of Isoquinoline‐1,3,4‐trione with Oxazoles.
Authors: Huang Chengmei , Yu Haitao , Miao Zhengrui , Zhou Jie , Wang Shuai , Fun Hoong‐Kun , Xu Jianhua , Zhang Yan 
Published: September 2011

AbstractIrradiation of isoquinoline‐1,3,4‐trione with the oxazoles (II) results in regio‐ and stereoselective formation of spiroisoquinolineoxetanes.
ChemInform Abstract: A New Rapid Multicomponent Domino Reaction for the Formation of Functionalized Benzo[h]pyrazolo[3,4‐b]quinolines.
Authors: Jiang Bo , Zhang Ge , Ma Ning , Shi Feng , Tu Shu‐Jiang , Kaur Parminder , Li Guigen 
Published: September 2011

AbstractBenzo[h]isoxazolo[5,4‐b]quinolines are also available.
ChemInform Abstract: Modular “Click” Sensors for Zinc and Their Application in vivo.
Authors: Jobe Kajally , Brennan Caroline H. , Motevalli Majid , Goldup Stephen M. , Watkinson Michael 
Published: September 2011

AbstractA novel cyclen‐based sensor (IV) is synthesized using a “click” approach which shows excellent selectivity for Zn2+ among other biologically relevant metals.
ChemInform Abstract: Ring‐Closing Metathesis of Sulfoximine‐Substituted N‐Tethered Trienes: Modular Asymmetric Synthesis of Medium‐Ring Nitrogen Heterocycles.
Authors: Mahajan Vishal , Gais Hans‐Joachim 
Published: September 2011

AbstractSulfoximine‐substituted N‐tethered trienes, synthesized from the corresponding homoallylic amines, undergo ruthenium‐catalyzed ring‐closing reaction to give the desired heterocycles with high diastereoselectivity.
ChemInform Abstract: Synthesis and Reactivities of Dihydrosilanes Tethered with Two Thioether Moieties.
Authors: Takeda Nobuhiro , Nakamura Tetsu , Imamura Ayako , Unno Masafumi 
Published: September 2011

AbstractThe reactions of the compound (IIb) with methanol, benzaldehyde, and carboxylic acids are tested, but no conversion is observed because of a very weak activation of the Si‐H bonds.
ChemInform Abstract: Effects of Heterole Spacers on the Structural, Optical, and Electrochemical Properties of 2,5‐Bis(1,5‐diphenylphosphol‐2‐yl)heteroles.
Authors: Matano Yoshihiro , Saito Arihiro , Fujita Masato , Imahori Hiroshi 
Published: September 2011

AbstractA convenient method for the synthesis of the title compounds (III), (IV), (VII), and (IX) is established.
ChemInform Abstract: Stereocomplementary Syntheses of 1,ω‐Distannylated E,Z‐Isomeric Conjugated Trienes, Tetraenes, and Pentaenes.
Authors: Burghart Jochen , Sorg Achim , Brueckner Reinhard 
Published: September 2011

AbstractThe synthesis is achieved by stereocontrolled C=C bond formation.
Impact of Diabetes Mellitus on the Clinical Management of Global Cardiovascular Risk: Analysis of the Results of the Evaluation of Final Feasible Effect of Control Training and Ultra Sensitization (EFFECTUS) Educational Program
Authors: Tocci Giuliano , Ferrucci Andrea , Guida Pietro , Avogaro Angelo , Comaschi Marco , Corsini Alberto , Cortese Claudio , Giorda Carlo Bruno , Manzato Enzo , Medea Gerardo , Mureddu Gian Francesco , Riccardi Gabriele , Titta Giulio , Ventriglia Giuseppe , Zito Giovanni Battista , Volpe Massimo ,   on behalf of the EFFECTUS Steering Committee
Published: September 2011

AbstractBackground:The Evaluation of Final Feasible Effect of Ultra Control Training and Sensitization (EFFECTUS) study is aimed at implementing global cardiovascular (CV) risk management in Italy.Hypothesis:To evaluate the impact of diabetes mellitus (DM) on attitudes and preferences for clinical management of global CV risk among physicians treating diabetic or nondiabetic patients.Methods:Involved physicians were asked to submit data into a study‐designed case‐report form, covering the first 10 adult outpatients consecutively seen in May 2006. All available clinical data were centrally analyzed for global CV risk assessment and CV risk profile characterization. Patients were stratified according to the presence or absence of DM.Results:Overall, 1078 physicians (27% female, ages 50 ± 7 y) collected data of 9904 outpatients (46.5% female, ages 67 ± 9 y), among whom 3681 (37%) had a diagnosis of DM at baseline. Diabetic patients were older and had higher prevalence of obesity, hypertension, dyslipidemia, and associated CV diseases than nondiabetic individuals (P<0.001). They had higher systolic blood pressure, total cholesterol, triglycerides, and creatinine levels, but lower high‐density lipoprotein cholesterol levels than nondiabetic patients (P<0.001). Higher numbers of blood pressure and lipid‐lowering drugs and antiplatelet agents were used in diabetic than in nondiabetic patients (P<0.001).Conclusions:The EFFECTUS study confirmed higher CV risk and more CV drug prescriptions in diabetic than in nondiabetic patients. Presence of DM at baseline significantly improved clinical data collection. Such an approach, however, was not paralleled by a better control of global CV risk profile, which was significantly worse in the former than in the latter group. © 2011 Wiley Periodicals, Inc.This work has been supported by an unconditioned educational grant by Merck Sharp & Dohme, Italy, for data collection and analysis. The authors have no other funding, financial relationships, or conflicts of interest to disclose.
Correlation Between Heart Rate Control During Exercise and Exercise Capacity in Patients With Chronic Atrial Fibrillation
Authors: Jaber Jefferson , Cirenza Claudio , Amaral Alessandro , Jaber Jeffrey , Oliveira Filho Japy A. , de Paola Angelo A. V. 
Published: September 2011

AbstractBackground:Rate control is an acceptable alternative to rhythm control in patients with chronic atrial fibrillation (AF).Hypothesis:The aim of this study of AF patients was to understand the correlation between their exercise capacity and both heart rate (HR) and HR variation index during exercise.Methods:The exercise capacity of 85 male patients with chronic AF was measured using a cardiopulmonary exercise test (CPX). Within this population, we compared the exercise tolerance of patients with a normal chronotropic response (maximal HR 85%–115% that of the maximal age‐predicted HR during CPX) to those whose HR response exceeded this range. Two similar comparisons were made by dividing the subject population according to (1) whether or not their HR variation index (HRVI) during CPX exceeded 10 bpm/min, and (2) whether their HR during the 6‐minute walk test exceeded 110 bpm.Results:Patients with an HRVI not over 10 bpm/min showed higher maximal oxygen uptake compared to patients with a higher HRVI (26.7 ± 6.1 vs 22.8 ± 4.8 mL O2/kg/min, P = 0.002) and a longer distance walked during CPX (705.6 ± 200.3 vs 520.9 ± 155.5 m, P<0.001). No other significant influence on exercise capacity was seen. Multivariate regression analysis revealed that both the body mass index and the HRVI during CPX were independent predictors of the maximal oxygen uptake.Conclusions:Better HRVI control on CPX was correlated with better exercise capacity in patients with chronic AF. © 2011 Wiley Periodicals, Inc.Jefferson Jaber, MD was supported by a fellowship grant from CNPq, Brazil. The authors have no other funding, financial relationships, or conflicts of interest to disclose.
Synthesis and Antimalarial Activities of a Diverse Set of Triazole‐Containing Furamidine Analogues
Authors: Berger Olivier , Kaniti Archana , van Ba Christophe Tran , Vial Henri , Ward Stephen A. , Biagini Giancarlo A. , Bray Patrick G. , O'Neill Paul M. 
Published: September 2011

AbstractFour different series of triazole diamidines have been prepared by the Pinner method from the corresponding triazole dinitriles. Copper‐catalyzed “click chemistry” was used for the synthesis of 1,4‐ and 4,5‐substituted triazoles, aryl magnesium acetylide reagents for the 1,5‐substituted triazoles, with a thermal dipolar addition reaction employed for the 2,4‐substituted triazoles. In vitro antimalarial activity against two different PfCRT‐modified parasite lines (Science 2002, 298, 210–213) of Plasmodium falciparum and inhibition of hemozoin formation were determined for each compound. Several diamidines with potent nanomolar antimalarial activities were identified, and selected molecules were resynthesized as their diamidoxime triazole prodrugs. One of these prodrugs, OB216, proved to be highly potent in vivo with an ED50 value of 5 mg kg−1 (po) and an observed 100 % cure rate (CD100) of just 10 mg kg−1 by oral (po) administration in mice infected with P. vinckei.