bluuebunny/crossref_metadata_2025_split huggingface dataset:
Upregulation of CRABP1 in human neuroblastoma cells overproducing the Alzheimer-typical Aβ42reduces their differentiation potential
Authors: Uhrig Markus , Brechlin Peter , Jahn Olaf , Knyazev Yuri , Weninger Annette , Busia Laura , Honarnejad Kamran , Otto Markus , Hartmann Tobias 
Published: June 2012

Abstract

Background
Alzheimer's disease (AD) is characterized by neurodegeneration and changes in cellular processes, including neurogenesis. Proteolytic processing of the amyloid precursor protein (APP) plays a central role in AD. Owing to varying APP processing, several β-amyloid peptides (Aβ) are generated. In contrast to the form with 40 amino acids (Aβ40), the variant with 42 amino acids (Aβ42) is thought to be the pathogenic form triggering the pathological cascade in AD. While total-Aβ effects have been studied extensively, little is known about specific genome-wide effects triggered by Aβ42 or Aβ40 derived from their direct precursor C99.

Methods
A combined transcriptomics/proteomics analysis was performed to measure the effects of intracellularly generated Aβ peptides in human neuroblastoma cells. Data was validated by real-time polymerase chain reaction (real-time PCR) and a functional validation was carried out using RNA interference.

Results
Here we studied the transcriptomic and proteomic responses to increased or decreased Aβ42 and Aβ40 levels generated in human neuroblastoma cells. Genome-wide expression profiles (Affymetrix) and proteomic approaches were combined to analyze the cellular response to the changed Aβ42- and Aβ40-levels. The cells responded to this challenge with significant changes in their expression pattern. We identified several dysregulated genes and proteins, but only the cellular retinoic acid binding protein 1 (CRABP1) was up-regulated exclusively in cells expressing an increased Aβ42/Aβ40 ratio. This consequently reduced all-trans retinoic acid (RA)-induced differentiation, validated by CRABP1 knock down, which led to recovery of the cellular response to RA treatment and cellular sprouting under physiological RA concentrations. Importantly, this effect was specific to the AD typical increase in the Aβ42/Aβ40 ratio, whereas a decreased ratio did not result in up-regulation of CRABP1.

Conclusion
We conclude that increasing the Aβ42/Aβ40 ratio up-regulates CRABP1, which in turn reduces the differentiation potential of the human neuroblastoma cell line SH-SY5Y, but increases cell proliferation. This work might contribute to the better understanding of AD neurogenesis, currently a controversial topic.
Plasminogen activator inhibitor type 1 regulates microglial motility and phagocytic activity
Authors: Jeon Hyejin , Kim Jong-Heon , Kim Jae-Hong , Lee Won-Ha , Lee Myung-Shik , Suk Kyoungho 
Published: June 2012

AbstractBackgroundPlasminogen activator inhibitor type 1 (PAI-1) is the primary inhibitor of urokinase type plasminogen activators (uPA) and tissue type plasminogen activators (tPA), which mediate fibrinolysis. PAI-1 is also involved in the innate immunity by regulating cell migration and phagocytosis. However, little is known about the role of PAI-1 in the central nervous system.MethodsIn this study, we identified PAI-1 in the culture medium of mouse mixed glial cells by liquid chromatography and tandem mass spectrometry. Secretion of PAI-1 from glial cultures was detected by ELISA and western blotting analysis. Cell migration was evaluated byin vitroscratch-wound healing assay or Boyden chamber assay and anin vivostab wound injury model. Phagocytic activity was measured by uptake of zymosan particles.ResultsThe levels of PAI-1 mRNA and protein expression were increased by lipopolysaccharide and interferon-γ stimulation in both microglia and astrocytes. PAI-1 promoted the migration of microglial cells in culture via the low-density lipoprotein receptor-related protein (LRP) 1/Janus kinase (JAK)/signal transducer and activator of transcription (STAT)1 axis. PAI-1 also increased microglial migrationin vivowhen injected into mouse brain. PAI-1-mediated microglial migration was independent of protease inhibition, because an R346A mutant of PAI-1 with impaired PA inhibitory activity also promoted microglial migration. Moreover, PAI-1 was able to modulate microglial phagocytic activity. PAI-1 inhibited microglial engulfment of zymosan particles in a vitronectin- and Toll-like receptor 2/6-dependent manner.ConclusionOur results indicate that glia-derived PAI-1 may regulate microglial migration and phagocytosis in an autocrine or paracrine manner. This may have important implications in the regulation of brain microglial activities in health and disease.
Distinct effects of calorie restriction on adipose tissue cytokine and angiogenesis profiles in obese and lean mice
Authors: Kurki Eveliina , Shi Jin , Martonen Essi , Finckenberg Piet , Mervaala Eero 
Published: June 2012

Abstract

Background
Obesity associates with low-grade inflammation and adipose tissue remodeling. Using sensitive high-throughput protein arrays we here investigated adipose tissue cytokine and angiogenesis-related protein profiles from obese and lean mice, and in particular, the influence of calorie restriction (CR).

Methods
Tissue samples from visceral fat were harvested from obese mice fed with a high-fat diet (60% of energy), lean controls receiving low-fat control diet as well as from obese and lean mice kept under CR (energy intake 70% of ad libitum intake) for 50 days. Protein profiles were analyzed using mouse cytokine and angiogenesis protein array kits.

Results
In obese and lean mice, CR was associated with 11.3% and 15.6% reductions in body weight, as well as with 4.0% and 4.6% reductions in body fat percentage, respectively. Obesity induced adipose tissue cytokine expressions, the most highly upregulated cytokines being IL-1ra, IL-2, IL-16, MCP-1, MIG, RANTES, C5a, sICAM-1 and TIMP-1. CR increased sICAM-1 and TIMP-1 expression both in obese and lean mice. Overall, CR showed distinct effects on cytokine expressions; in obese mice CR largely decreased but in lean mice increased adipose tissue cytokine expressions. Obesity was also associated with increased expressions of angiogenesis-related proteins, in particular, angiogenin, endoglin, endostatin, endothelin-1, IGFBP-3, leptin, MMP-3, PAI-1, TIMP-4, CXCL16, platelet factor 4, DPPIV and coagulation factor III. CR increased endoglin, endostatin and platelet factor 4 expressions, and decreased IGFBP-3, NOV, MMP-9, CXCL16 and osteopontin expressions both in obese and lean mice. Interestingly, in obese mice, CR decreased leptin and TIMP-4 expressions, whereas in lean mice their expressions were increased. CR decreased MMP-3 and PAI-1 only in obese mice, whereas CR decreased FGF acidic, FGF basic and coagulation factor III, and increased angiogenin and DPPIV expression only in lean mice.

Conclusions
CR exerts distinct effects on adipocyte cytokine and angiogenesis profiles in obese and lean mice. Our study also underscores the importance of angiogenesis-related proteins and cytokines in adipose tissue remodeling and development of obesity.
Pain after earthquake
Authors: Angeletti Chiara , Guetti Cristiana , Papola Roberta , Petrucci Emiliano , Ursini Maria Laura , Ciccozzi Alessandra , Masi Francesca , Russo Maria Rosaria , Squarcione Salvatore , Paladini Antonella , Pergolizzi Joseph , Taylor Robert , Varrassi Giustino , Marinangeli Franco 
Published: June 2012

Abstract

Introduction
On 6 April 2009, at 03:32 local time, an Mw 6.3 earthquake hit the Abruzzi region of central Italy causing widespread damage in the City of L Aquila and its nearby villages. The earthquake caused 308 casualties and over 1,500 injuries, displaced more than 25,000 people and induced significant damage to more than 10,000 buildings in the L'Aquila region.

Objectives
This observational retrospective study evaluated the prevalence and drug treatment of pain in the five weeks following the L'Aquila earthquake (April 6, 2009).

Methods
958 triage documents were analysed for patients pain severity, pain type, and treatment efficacy.

Results
A third of pain patients reported pain with a prevalence of 34.6%. More than half of pain patients reported severe pain (58.8%). Analgesic agents were limited to available drugs: anti-inflammatory agents, paracetamol, and weak opioids. Reduction in verbal numerical pain scores within the first 24 hours after treatment was achieved with the medications at hand. Pain prevalence and characterization exhibited a biphasic pattern with acute pain syndromes owing to trauma occurring in the first 15 days after the earthquake; traumatic pain then decreased and re-surged at around week five, owing to rebuilding efforts. In the second through fourth week, reports of pain occurred mainly owing to relapses of chronic conditions.

Conclusions
This study indicates that pain is prevalent during natural disasters, may exhibit a discernible pattern over the weeks following the event, and current drug treatments in this region may be adequate for emergency situations.
Validation of ACR/EULAR definition of remission in rheumatoid arthritis from RA practice: the ESPOIR cohort
Authors: Zhang Bin , Combe Bernard , Rincheval Nathalie , Felson David T 
Published: June 2012

Abstract
Introduction
In development of the American College of Rheumatology (ACR)/European League Against Rheumatism (EULAR) rheumatoid arthritis (RA) remission definitions using clinical trials data, one criterion used to compare different remission definitions was whether, compared with those not in remission, those in remission had evidence of later disease stability defined by x-ray and functional status. Validation of the RA remission criteria using observational study data is necessary before recommending their use in practice.

Methods
Using data from those who met RA criteria in the ESPOIR cohort, we matched each person in remission with a person not in remission and then carried out analyses comparing later stability of x-ray and health assessment questionnaire (HAQ) between the two groups. We compared the predictive validity of the same candidate definitions of remission evaluated in the ACR/EULAR process. To minimize potential bias and produce more stable results, we used a bootstrap resampling approach to select those not in remission, repeating the sample matching analysis process 500 times.

Results
Results were similar to those of clinical trials analyzed for the ACR/EULAR remission criteria. Specifically, the ACR/EULAR remission definitions using either an simple disease activity index (SDAI) ≤ 3.3, clinical disease activity index (CDAI) ≤ 2.8 or a definition of remission requiring tender joint count, swollen joint count, patient global assessment all ≤ 1 performed as well or better than other candidate definitions of remission in terms of predicting later x-ray and function stability.

Conclusions
ACR/EULAR definitions of remission developed for trials are similarly valid in observational studies in RA and could be used in practice.
Clinical validation of a new thermodilution system for the assessment of cardiac output and volumetric parameters
Authors: Kiefer Nicholas , Hofer Christoph K , Marx Gernot , Geisen Martin , Giraud Raphaël , Siegenthaler Nils , Hoeft Andreas , Bendjelid Karim , Rex Steffen 
Published: June 2012

Abstract
Introduction
Transpulmonary thermodilution is used to measure cardiac output (CO), global end-diastolic volume (GEDV) and extravascular lung water (EVLW). A system has been introduced (VolumeView/EV1000™ system, Edwards Lifesciences, Irvine CA, USA) that employs a novel algorithm for the mathematical analysis of the thermodilution curve. Our aim was to evaluate the agreement of this method with the established PiCCO™ method (Pulsion Medical Systems SE, Munich, Germany, clinicaltrials.gov identifier: NCT01405040)

Methods
Seventy-two critically ill patients with clinical indication for advanced hemodynamic monitoring were included in this prospective, multicenter, observational study. During a 72-hour observation period, 443 sets of thermodilution measurements were performed with the new system. These measurements were electronically recorded, converted into an analog resistance signal and then re-analyzed by a PiCCO2™ device (Pulsion Medical Systems SE).

Results
For CO, GEDV, and EVLW, the systems showed a high correlation (r2 = 0.981, 0.926 and 0.971, respectively), minimal bias (0.2 L/minute, 29.4 ml and 36.8 ml), and a low percentage error (9.7%, 11.5% and 12.2%). Changes in CO, GEDV and EVLW were tracked with a high concordance between the two systems, with a traditional concordance for CO, GEDV, and EVLW of 98.5%, 95.1%, and 97.7% and a polar plot concordance of 100%, 99.8% and 99.8% for CO, GEDV, and EVLW, respectively. Radial limits of agreement for CO, GEDV and EVLW were 0.31 ml/minute, 81 ml and 40 ml, respectively. The precision of GEDV measurements was significantly better using the VolumeView™ algorithm compared to the PiCCO™ algorithm (0.033 (0.03) versus 0.040 (0.03; median (interquartile range), P = 0.000049).

Conclusions
For CO, GEDV, and EVLW, the agreement of both the individual measurements as well as measurements of change showed the interchangeability of the two methods. For the VolumeView method, the higher precision may indicate a more robust GEDV algorithm.

Trial registration
clinicaltrials.gov NCT01405040.
Fluid overload and changes in serum creatinine after cardiac surgery: predictors of mortality and longer intensive care stay. A prospective cohort study
Authors: Stein Anna , de Souza Lucas Vieira , Belettini Cassian Rodrigues , Menegazzo Willian Roberto , Viégas Júlio Rosales , Costa Pereira Edemar Manuel , Eick Renato , Araújo Lilian , Consolim-Colombo Fernanda , Irigoyen Maria Cláudia 
Published: June 2012

Abstract
Introduction
Fluid overload is a clinical problem frequently related to cardiac and renal dysfunction. The aim of this study was to evaluate fluid overload and changes in serum creatinine as predictors of cardiovascular mortality and morbidity after cardiac surgery.

Methods
Patients submitted to heart surgery were prospectively enrolled in this study from September 2010 through August 2011. Clinical and laboratory data were collected from each patient at preoperative and trans-operative moments and fluid overload and creatinine levels were recorded daily after cardiac surgery during their ICU stay. Fluid overload was calculated according to the following formula: (Sum of daily fluid received (L) - total amount of fluid eliminated (L)/preoperative weight (kg) × 100). Preoperative demographic and risk indicators, intra-operative parameters and postoperative information were obtained from medical records. Patients were monitored from surgery until death or discharge from the ICU. We also evaluated the survival status at discharge from the ICU and the length of ICU stay (days) of each patient.

Results
A total of 502 patients were enrolled in this study. Both fluid overload and changes in serum creatinine correlated with mortality (odds ratio (OR) 1.59; confidence interval (CI): 95% 1.18 to 2.14, P = 0.002 and OR 2.91; CI: 95% 1.92 to 4.40, P <0.001, respectively). Fluid overload played a more important role in the length of intensive care stay than changes in serum creatinine. Fluid overload (%): b coefficient = 0.17; beta coefficient = 0.55, P <0.001); change in creatinine (mg/dL): b coefficient = 0.01; beta coefficient = 0.11, P = 0.003).

Conclusions
Although both fluid overload and changes in serum creatinine are prognostic markers after cardiac surgery, it seems that progressive fluid overload may be an earlier and more sensitive marker of renal dysfunction affecting heart function and, as such, it would allow earlier intervention and more effective control in post cardiac surgery patients.
Spin Crossover and Light-induced Excited Spin-state Trapping Observed for an Iron(II) Complex Chelated with Tripodal Tetrakis(2-pyridyl)methane
Authors: Hirosawa Naoki , Oso Yuya , Ishida Takayuki 
Published: June 2012

Abstract
A novel iron(II) complex [Fe(py4C)2][Fe(py4C)(NCS)3]2 was synthesized, where py4C stands for tetrakis(2-pyridyl)methane. Spin crossover occurred at 161 K (T1/2). Light-induced excited spin-state trapping was observed when irradiated with 532 nm at 10 K, and the conversion was 89%. The relaxation took place at 58 K on heating.
Isolation and Structure of a Novel Biindole Pigment Substituted with an Ethyl Group from a Metagenomic Library Derived from the Marine Sponge Halichondria okadai
Authors: Abe Takahiro , Kukita Akira , Akiyama Kiyotaka , Naito Takayuki , Uemura Daisuke 
Published: June 2012

Abstract
We screened a colored clone from a metagenomic library derived from the marine sponge Halichondria okadai. We isolated a yellow pigment, halichrome A (1), which was structurally elucidated to be a biindole, exhibited cytotoxicity against B16 melanoma cells and was substituted with an ethyl group. To the best of our knowledge, this is the first report of the isolation of a novel compound from a metagenomic library derived from a marine sponge.
Repeatable Wettability Conversion between Hydrophobic and Superhydrophilic States on Nonwoven Fabrics Based on Electrospun TiO2–Poly(dimethylsiloxane) Fibers
Authors: Nakata Kazuya , Sakai Munetoshi , Liu Baoshun , Ochiai Tsuyoshi , Murakami Taketoshi , Fujishima Akira 
Published: June 2012

Abstract
Nonwoven fabrics based on composite fibers of TiO2 and poly(dimethylsiloxane) (PDMS) are prepared by electrospinning. Nonwoven fabrics based on TiO2–PDMS fibers are initially hydrophobic and undergo rapid wettability conversion to a superhydrophilic state upon plasma irradiation. Hydrophobicity is rapidly recovered under ambient conditions. This wettability conversion can be repeated by subsequent cycles of plasma irradiation and storage under ambient conditions.
Pt-Catalyzed Oxidative Rearrangement of Cyclic Tertiary Allylic Alcohols to Enones Using Aqueous Hydrogen Peroxide
Authors: Nagamine Takashi , Kon Yoshihiro , Sato Kazuhiko 
Published: June 2012

Abstract
An oxidative rearrangement of cyclic tertiary allylic alcohols to β-disubstituted α,β-unsaturated ketones by Pt black catalyst with aqueous hydrogen peroxide is described. The reaction proceeds under organic solvent- and halide-free conditions and gives only water as a coproduct. The Pt black catalyst is commercially available and can be reused at least four times.
Estimation of Ultimate Lateral Load Capacity of Piles in Sands Using Calibration Chamber Tests
Authors: Lee Junhwan , Paik Kyuho , Kim Daehong , Park Donggyu 
Published: June 2012

Abstract
Current practice for the estimation of the ultimate lateral load capacity of piles is typically based on the vertical effective stress σ′v, while the effect of the lateral effective stress σ′h is not specifically considered. In the present study, calibration chamber lateral pile load tests are conducted to investigate the load response and ultimate lateral load capacity Hu of laterally loaded piles under various soil and stress conditions. In order to determine Hu from load-deflection curves, different criteria are explored and analyzed. From the test results, it is shown that Hu increases significantly with increasing σ′h for a given σ′v. It is also found that lateral deflection of pile at ultimate state tends to increase as the relative density and lateral stress increase. On the basis of the test results, the lateral stress correction factor reflecting the effect of the lateral effective stress σ′h on Hu is proposed. From the test results, it is seen that the proposed procedure using the lateral stress correction factor produces more realistic estimation of Hu. Case examples are selected from the literature and used to compare results measured and predicted using the proposed approach.
Education in Accountancy and Social Control: Questions and Comments
Authors: Loeb Stephen E. 
Published: June 2012

ABSTRACTMy paper considers two questions relating to the roles that education in college and university degree programs and in continuing professional education (CPE) programs play in the social control of the public accounting profession. These questions consider how: (1) educators should respond to the possibility that outside stakeholders might misinterpret the effect of ethics education in accounting degree programs in colleges and universities and ethics-related CPE, and (2) CPE should be used in the public accounting profession's disciplinary process. An assessment process and content similarity are suggested as ways to approach issues raised by the first question. CPE is most appropriate as a sanction when a Certified Public Accountant (CPA) in public accounting unintentionally violates an ethical standard of the public accounting profession and an effort is being made to rehabilitate the CPA. However, if a CPA in public accounting intentionally violates an ethical standard of the public accounting profession, the appropriateness of using CPE as a sanction in an effort to rehabilitate the CPA is controversial and requires research. Both questions and their discussion in the paper draw on comments in Waithe and Ozar (1990).
Surgical approach of complicated diverticulitis with colovesical fistula: technical note in a particular condition
Authors: Desiderio Jacopo , Trastulli Stefano , Listorti Chiara , Milani Diego , Cerroni Michele , Cochetti Giovanni , Cirocchi Roberto , Boselli Carlo , Parisi Amilcare , Mearini Ettore , Noya Giuseppe 
Published: June 2012

Abstract
Background: Diverticular disease of the colon is common in the Western world. With the first episode of diverticulitis, most patients will benefit from medical therapy, but in 10% to 20% of cases some complications will develop, such as intra-abdominal abscesses, obstructions, fistulas. In these conditions it is important to define the most appropriate surgical approach. Discussion: The management of diverticular disease has been successful owing to the advances in diagnostic methods, intensive care and surgical experience, but there is debate about the best treatment for some conditions. Fistulas complicating diverticulitis are the result of a localized perforation into adjacent viscera. In particular, the connection between the colon and the urinary tract is a serious anatomical abnormality that must be urgently corrected before a serious urinary infection results. Indications, timing and surgical procedures are determined by the severity of the disease and the patient’s general condition. Summary: Diverticular disease can lead to many complications. One of the most difficult to correct is an internal fistula, such as a colo-vesical fistula. The correct approach in cases where the disorder is clinically suspected has always been controversial, and the guidelines for sigmoid diverticulitis have not established the most appropriate method for diagnosis and treatment. At present, the surgical strategy for these cases requires interruption of the fistula and resection to remove the inflamed colonic segment, with or without primary anastomosis, focusing attention on the construction of the anastomosis to well vascularized and anatomically healthy tissues. It is clear, therefore, that establishing guidelines is difficult, because many pathological situations may be related to diverticulitis, and so, as our experience shows, the surgical approach has to be tailored to the patient’s general and local condition.