bluuebunny/crossref_metadata_2025_split huggingface dataset:
Analysis of Fatty Acids in 12 Mediterranean Fish Species: Advantages and Limitations of a New GC‐FID/GC–MS Based Technique
Authors: Nevigato Teresina , Masci Maurizio , Orban Elena , Di Lena Gabriella , Casini Irene , Caproni Roberto 
Published: May 2012

AbstractWhen fatty acids in fish are analyzed, results in percentage form (profile analysis) are mostly reported. However, the much more useful results expressed as mg/100 g (absolute analysis) is the main information required. Absolute methods based on calibration curves are of good accuracy but with a high degree of complexity if applied to a great number of analytes. Procedures based on the sequence profile analysis–total FA determination–absolute analysis may be suitable for routine use, but suffer from a number of uncertainties that have never been really resolved. These uncertainties are mainly related to the profile analysis. In fact, most profile analyses reported in the literature disagree about the number and type of fatty acids monitored as well as about the total percentage to assign to their sum so leading to possible inaccuracies; in addition the instrumental response factor for all FAME (fatty acid methyl esters) is often considered as a constant, but this is not exactly true. In this work, a set of 24 fatty acids was selected and studied on 12 fish species in the Mediterranean area (variable in lipid content and month of sampling): in our results, and in these species, this set constitutes, on average, 90 ± 3 % of the total fatty acid content. Moreover the error derived from the assumption of a unique response factor was investigated. Two different detection techniques (GC‐FID and GC–MS) together with two capillary columns (different in length and polarity) were used in order to acquire complementary data on the same sample. With the protocol here proposed absolute analyses on the 12 cited species are easily achievable by the total FA determination procedure. The accuracy of this approach is good in general, but in some cases (DHA for example) is lower than the accuracy of calibration‐based methods. The differences were evaluated on a case by case basis.
Formation of columnar baroclinic vortices in thermally stratified nonlinear spin-up
Authors: Pacheco J. R. , Verzicco R. 
Published: May 2012

AbstractWe investigate the mechanisms that affect the formation of columnar vortices for spin-up in a cylinder where the temperatures at the horizontal walls are prescribed. Numerical results from the three-dimensional Navier–Stokes equations show that a short-lived instability, suppressed by the combined effect of rotation and stratification, generates small temperature variations in the azimuthal direction. Temperature-gradient anomalies produce vorticity, and these vortices stir the fluid at the interface of the central vortex core thus reinforcing the temperature gradients. For sufficiently strong temperature gradients, the central vortex core breaks up into several columnar vortices. It is found, in particular, that small aspect ratios (height over radius of the cylindrical fluid layer) $\Gamma = 1, 2$ tend to inhibit the instability, while larger ones, $\Gamma = 3. 3$, have the opposite effect. The main source of instability is the baroclinic vorticity production and not the presence of a solid sidewall since, counter-intuitively, the flow is more unstable for a free-slip boundary than for a no-slip one. Finally the effect of the temperature boundary conditions (isothermal versus adiabatic) on the horizontal boundaries has been investigated. The adiabatic boundaries help to preserve for longer times the sloping density interfaces that feed, with their potential energy, the baroclinic vorticity production; this results in more unstable flows.
Minimal seeds for shear flow turbulence: using nonlinear transient growth to touch the edge of chaos
Authors: Pringle Chris C. T. , Willis Ashley P. , Kerswell Rich R. 
Published: May 2012

AbstractWe propose a general strategy for determining the minimal finite amplitude disturbance that triggers transition to turbulence in shear flows. This involves constructing a variational problem that searches over all disturbances of fixed initial amplitude which respect the boundary conditions, incompressibility and the Navier–Stokes equations, to maximize a chosen functional over an asymptotically long time period. The functional must be selected such that it identifies turbulent velocity fields by taking significantly enhanced values compared to those for laminar fields. We illustrate this approach using the ratio of the final to initial perturbation kinetic energies (energy growth) as the functional and the energy norm to measure amplitudes in the context of pipe flow. Our results indicate that the variational problem yields a smooth converged solution provided that the initial amplitude is below the threshold for transition. This optimal is the nonlinear analogue of the well-studied (linear) transient growth optimal. At the critical threshold, the optimization seeks out a disturbance that is on the ‘edge’ of turbulence during the period. Above this threshold, when disturbances trigger turbulence by the end of the period, convergence is then practically impossible. The first disturbance found to trigger turbulence as the amplitude is increased identifies the ‘minimal seed’ for the given geometry and forcing (Reynolds number). We conjecture that it may be possible to select a functional such that the converged optimal below threshold smoothly converges to the minimal seed at threshold. Our choice of the energy growth functional is shown to come close to this for the pipe flow geometry investigated here.
Specific micronutrient concentrations are associated with inflammatory cytokines in a rural population of Mexican women with a high prevalence of obesity
Authors: Zavala Gerardo , Long Kurt Z. , García Olga P. , Caamaño María del Carmen , Aguilar Tania , Salgado Luis M. , Rosado Jorge L. 
Published: May 2012

It has been recognised recently that obese individuals have lower concentrations of micronutrients and this may affect the concentrations of inflammatory cytokines. A cross-sectional study was carried out to evaluate the association of specific micronutrients' status with chronic inflammation caused by obesity in 280 women (36·1 (sd7·5) years) from seven rural communities in Mexico. Measurements of weight, height and waist circumference were made on all women and body composition was determined by dual-energy X-ray absorptiometry. Concentrations of the cytokines IL-1, TNF-α, IL-6, IL-10 and IL-12, lipid profile, and the micronutrients Zn and vitamins A, C and E were determined in fasting blood samples. Ordered logistic regression models were used to determine associations between categorised cytokine levels and micronutrients. It was found that 80 % of women were overweight or obese, and had significantly higher concentrations of C-reactive protein than normal-weight women (P= 0·05). The risk of higher levels of TNF-α, IL-6, IL-10 and IL-12 was reduced significantly among women with higher Zn concentrations (OR 0·63, 95 % CI 0·42, 0·96,P= 0·03; OR 0·57, 95 % CI 0·39, 0·86,P= 0·025; OR 0·63, 95 % CI 0·41, 0·96,P= 0·04; OR 0·62, 95 % CI 0·41, 0·95,P= 0·03, respectively). Higher concentrations of vitamin A were slightly associated with reduced risks of higher levels of IL-1 and IL-12 (OR 0·97, 95 % CI 0·95, 0·99,P= 0·03; OR 0·97, 95 % CI 0·94, 0·99,P= 0·03, respectively); when adjusting for BMI, this association was lost. No associations were found between vitamin C or vitamin E:lipids concentrations and inflammatory cytokines. In conclusion, higher Zn concentrations are associated with reduced risks of higher concentration of inflammation markers in a population of women with a high prevalence of obesity.
Spontaneous pneumocephalus presenting with alien limb phenomena
Authors: Nash R , Wilson M , Adams M , Kitchen N 
Published: May 2012

AbstractBackground:Spontaneous pneumocephalus is a rare condition that has been reported infrequently. Alien limb syndrome is an uncommon phenomenon most often seen in patients with frontal and callosal lesions.Method:Case report of a patient with pneumocephalus presenting with alien limb syndrome. The patient underwent successful surgical management. A literature review and discussion of aspects of this presentation are also included.Conclusion:In this case, a spontaneous pneumocephalus has formed a frontal space-occupying lesion and presented with alien limb phenomena.
The EU, Global Europe, and processes of uneven and combined development: the problem of transnational labour solidarity
Authors: BIELER ANDREAS 
Published: May 2012

AbstractIn 2006, the European Union launched its new free trade strategy Global Europe with the explicit goal of increasing European competitiveness. This article explores the positions of trade unions and other social movements on Global Europe. Importantly, while Northern social movements and trade unions from the Global South reject Global Europe due to its impact of deindustrialisation on developing countries, European trade unions support it in so far as it opens up new markets for the export of European manufactured goods. It will be argued that this has to be understood against the background of the dynamics underlying the global economy and here in particular uneven and combined development. Due to the uneven integration of different parts of the world into the global economy, workers in developed countries may actually benefit from free trade, while workers in the Global South are more likely to lose out. It will, however, also be argued that while these different positions within the social relations of production are shaping the position of trade unions, they do not determine them. Over time, through direct engagement, trade unions in the North and South may be able to establish relations of transnational solidarity.
A non-sovereign modernity: attempts to engineer stability in the Balkans 1820–90
Authors: HALDÉN PETER 
Published: May 2012

AbstractSocial theory almost invariably equates modernity with the sovereign state. This equation must be nuanced because the modern era and modern strategies of international stability have contained non-sovereign units. In the nineteenth century, the Great Powers tried to create international stability by engineering forms of rule in Europe. These strategies built on distinctively modern ideas: the possibility of radically breaking with the past, redesigning political organisations, and actively controlling political events through rational planning. Throughout the century the Great Powers alternated between creating non-sovereign units and creating sovereign units as instruments in these stabilising strategies. The degree of trust between the Great Powers accounts for the shift between the two strategies: they tended to create non-sovereign units when mutual trust was high and sovereign ones when trust was low. This article analyses Great Power strategies of designing forms of rule in the Balkans between 1820 and 1878. Like in previous centuries, nineteenth-century Europe actually consisted of two parallel but connected systems: the egalitarian system of sovereign states and a system of non-sovereign entities. Non-sovereign units disappeared only late in the century and this process was affected by the increasing rivalry and mistrust between the sovereign states.
Scoring models of a diet quality index and the predictive capability of mortality in a population-based cohort of Swedish men and women
Authors: Drake Isabel , Gullberg Bo , Sonestedt Emily , Wallström Peter , Persson Margaretha , Hlebowicz Joanna , Nilsson Jan , Hedblad Bo , Wirfält Elisabet 
Published: May 2012

AbstractObjectiveTo examine how different scoring models for a diet quality index influence associations with mortality outcomes.DesignA study within the Malmö Diet and Cancer cohort. Food and nutrient intakes were estimated using a diet history method. The index included six components: SFA, PUFA, fish and shellfish, fibre, fruit and vegetables, and sucrose. Component scores were assigned using predefined (based on dietary recommendations) and population-based cut-offs (based on median or quintile intakes). Multivariate Cox regression was used to model associations between index scores (low, medium, high) and all-cause and cause-specific mortality by sex.SettingMalmö, the third largest city in Sweden.SubjectsMen (n 6940) and women (n 10 186) aged 44–73 years. During a mean follow-up of 14·2 years, 2450 deaths occurred, 1221 from cancer and 709 from CVD.ResultsThe predictive capability of the index for mortality outcomes varied with type of scoring model and by sex. Stronger associations were seen among men using predefined cut-offs. In contrast, the quintile-based scoring model showed greater predictability for mortality outcomes among women. The scoring model using median-based cut-offs showed low predictability for mortality among both men and women.ConclusionsThe scoring model used for dietary indices may have a significant impact on observed associations with disease outcomes. The rationale for selection of scoring model should be included in studies investigating the association between dietary indices and disease. Adherence to the current dietary recommendations was in the present study associated with decreased risk of all-cause and cause-specific mortality, particularly among men.
Identifying Hexagonal Boron Nitride Monolayers by Transmission Electron Microscopy
Authors: Odlyzko Michael L. , Mkhoyan K. Andre 
Published: May 2012

AbstractMultislice simulations in the transmission electron microscope (TEM) were used to examine changes in annular-dark-field scanning TEM (ADF-STEM) images, conventional bright-field TEM (BF-CTEM) images, and selected-area electron diffraction (SAED) patterns as atomically thin hexagonal boron nitride (h-BN) samples are tilted up to 500 mrad off of the [0001] zone axis. For monolayer h-BN the contrast of ADF-STEM images and SAED patterns does not change with tilt in this range, while the contrast of BF-CTEM images does change; h-BN multilayer contrast varies strongly with tilt for ADF-STEM imaging, BF-CTEM imaging, and SAED. These results indicate that tilt series analysis in ADF-STEM image mode or SAED mode should permit identification of h-BN monolayers from raw TEM data as well as from quantitative post-processing.
Isolation and Molecular Characterization of a Novel Infectious Pancreatic Necrosis Virus Strain in Returning Atlantic Salmon Salmo salar from the Connecticut River, USA
Authors: Glenney Gavin W. , Barbash Patricia A. , Coll John A. , Quartz William M. 
Published: May 2012

Abstract
After 22 years of negative viral screening results, the viral pathogen infectious pancreatic necrosis virus (IPNV) was isolated from the ovarian fluid of two pooled samples of returning Connecticut River Atlantic salmon Salmo salar during the 2007 spawning season at Richard Cronin National Salmon Station (RCNSS), Hadley, Massachusetts. Cytopathic effect was observed in Chinook salmon embryo (CHSE-214) cells, and IPNV was confirmed by reverse transcriptase–polymerase chain reaction (RT–PCR). Sequence analysis conducted by the U.S. Geological Survey's Western Fisheries Research Center determined that the isolate closely resembled the Canada_3 strain, falling into Genogroup 4 rather than Genogroup 1, which is more common in the United States. This allowed us to speculate that the Atlantic salmon were not infected during their freshwater life stage in the Connecticut River watershed but somewhere on their migratory route or feeding grounds in the Northwest Atlantic. On November 20, 2007, the Connecticut River Atlantic Salmon Commission voted to depopulate the infected stock at RCNSS and the entire suspect egg lots held at White River National Fish Hatchery, Vermont. Approximately one and a half months later, the 121 Connecticut River Atlantic salmon were euthanized and sampled for a follow-up investigation to determine the prevalence of infection. Only one kidney–spleen homogenate (male) was confirmed IPNV positive via cell culture and RT–PCR. A total of 2,983 base pairs from segment A of the RNA genome were sequenced from this fish and determined to be from a new strain (Connecticut-1) of IPNV that closely resembles Canada_2 and Canada_3 in Genogroup 4. The new strain is genetically identical to one of the first ovarian fluid isolates over a shared 130-nucleotide region, possibly indicating original transmission from a single source. The absence of IPNV from the Connecticut River's subsequent four returning Atlantic salmon year-classes may indicate that the aggressive corrective action was prudent.
Received April 5, 2011; accepted December 9, 2011
Effects of Metomidate Hydrochloride Sedation on Blood Glucose and Marketability of Transported Threespot Gourami Trichogaster trichopterus
Authors: Crosby Tina C. , Hill Jeffrey E. , Hartman Kathleen H. , Yanong Roy P. E. 
Published: May 2012

Abstract
Our objectives were to determine whether sedation with metomidate hydrochloride (hereafter, “metomidate”) during transportation of threespot gourami Trichogaster trichopterus would prevent an increase in blood glucose levels and improve fish marketability (i.e., based on appearance and behavior) in comparison with unsedated controls. Threespot gourami are obligate air-breathers that possess a labyrinth organ, enabling the fish to respire air above the water surface; these fish should be lightly sedated during transport. Fish were transported for approximately 24 h via truck and domestic airline. Blood was sampled at 0, 2, 6, and 12 h posttransportation, and appearance and behavior were observed at 0, 2, 4, 6, and 12 h and 7 d posttransportation. Metomidate concentrations tested were 0.0 (control), 0.1, 0.2, 0.3, and 0.4 mg/L. At the concentrations tested, metomidate neither inhibited elevations in blood glucose nor improved marketability. Fish that were transported with 0.3-mg/L metomidate were less marketable based on behavioral indices, and fish that were transported with 0.4-mg/L metomidate had higher glucose levels than control fish. Use of metomidate as a transport sedative for threespot gourami should be considered with caution and may be problematic at the concentrations tested; however, further research examining additional indices of stress may clarify metomidate use for this species.
Received February 16, 2011; accepted December 18, 2011.
BATMAN—an R package for the automated quantification of metabolites from nuclear magnetic resonance spectra using a Bayesian model
Authors: Hao Jie , Astle William , De Iorio Maria , Ebbels Timothy M D 
Published: May 2012

Abstract
Motivation: Nuclear Magnetic Resonance (NMR) spectra are widely used in metabolomics to obtain metabolite profiles in complex biological mixtures. Common methods used to assign and estimate concentrations of metabolites involve either an expert manual peak fitting or extra pre-processing steps, such as peak alignment and binning. Peak fitting is very time consuming and is subject to human error. Conversely, alignment and binning can introduce artefacts and limit immediate biological interpretation of models.
Results: We present the Bayesian automated metabolite analyser for NMR spectra (BATMAN), an R package that deconvolutes peaks from one-dimensional NMR spectra, automatically assigns them to specific metabolites from a target list and obtains concentration estimates. The Bayesian model incorporates information on characteristic peak patterns of metabolites and is able to account for shifts in the position of peaks commonly seen in NMR spectra of biological samples. It applies a Markov chain Monte Carlo algorithm to sample from a joint posterior distribution of the model parameters and obtains concentration estimates with reduced error compared with conventional numerical integration and comparable to manual deconvolution by experienced spectroscopists.
Availability and implementation: http://www1.imperial.ac.uk/medicine/people/t.ebbels/
Contact: t.ebbels@imperial.ac.uk
Gowinda: unbiased analysis of gene set enrichment for genome-wide association studies
Authors: Kofler Robert , Schlötterer Christian 
Published: May 2012

Abstract
Summary: An analysis of gene set [e.g. Gene Ontology (GO)] enrichment assumes that all genes are sampled independently from each other with the same probability. These assumptions are violated in genome-wide association (GWA) studies since (i) longer genes typically have more single-nucleotide polymorphisms resulting in a higher probability of being sampled and (ii) overlapping genes are sampled in clusters. Herein, we introduce Gowinda, a software specifically designed to test for enrichment of gene sets in GWA studies. We show that GO tests on GWA data could result in a substantial number of false-positive GO terms. Permutation tests implemented in Gowinda eliminate these biases, but maintain sufficient power to detect enrichment of GO terms. Since sufficient resolution for large datasets requires millions of permutations, we use multi-threading to keep computation times reasonable.
Availability and implementation: Gowinda is implemented in Java (v1.6) and freely available on http://code.google.com/p/gowinda/
Contact: christian.schloetterer@vetmeduni.ac.at
Supplementary information: Manual: http://code.google.com/p/gowinda/wiki/Manual. Test data and tutorial: http://code.google.com/p/gowinda/wiki/Tutorial. Validation: http://code.google.com/p/gowinda/wiki/Validation.
Under‐recognized complications in patients with paroxysmal nocturnal haemoglobinuria: raised pulmonary pressure and reduced right ventricular function
Authors: Hill Anita , Sapsford Robert J. , Scally Andrew , Kelly Richard , Richards Stephen J. , Khurisgara Gus , Sivananthan Mohan U. , Hillmen Peter 
Published: May 2012

SummaryPulmonary hypertension is becoming a recognized complication of the hereditary and acquired haemolytic anaemias, associated with a poor prognosis. Recently we reported that patients with paroxysmal nocturnal haemoglobinuria (PNH) have high levels of N‐terminal pro‐brain natriuretic peptide (NT‐proBNP), a biomarker associated with both right and left ventricular dysfunction and cardiac dysfunction. In the current study we evaluated a cohort of patients (N = 29) with haemolytic PNH for elevated pulmonary artery systolic pressure and cardiac function by Doppler‐echocardiography. Of the 29 patients, eight were further studied using cardiac magnetic resonance imaging (MRI), as well as two additional patients (number of patients studied using cardiac MRI = 10). Plasma from the first cohort (N = 29) demonstrated intravascular haemolysis associated with a 12‐fold increase in median nitric oxide (NO) consumption when compared with healthy volunteers (P < 0·001). Doppler echocardiography demonstrated normal left ventricular function and elevated pulmonary artery systolic pressure in 41% of patients. Cardiac MRI from the second cohort (N = 10) demonstrated depressed right ventricular function in 80% of PNH patients tested, and 60% had findings suggestive of subclinical small pulmonary emboli. Together, these data suggest a high prevalence of haemolysis‐associated NO scavenging, Doppler‐estimated systolic pulmonary hypertension, and depressed right ventricular function in patients with PNH.