bluuebunny/crossref_metadata_2025_split huggingface dataset:
A critical examination of environmental public interest litigation in China - reflection on China’s environmental authoritarianism
Authors: Li Xin , Song Zongyue 
Published: May 2024

AbstractEnvironmental public interest litigation is an innovative legal mechanism for humanity to address environmental crises. It not only addresses the tragedy of the commons in environmental crises but also serves as a crucial means for protecting the rights of environmentally vulnerable groups and upholding environmental justice. Over the past decade, the development of China’s environmental public interest litigation system has been promising. Thousands of such cases are filed each year, making a significant contribution to curbing the further deterioration of China’s environmental crisis. However, China still does not allow individual citizens to initiate environmental public interest litigation, and there are significant hurdles for environmental NGOs to file such lawsuits. As a result, the vast majority of environmental public interest litigation cases in China are initiated by procuratorates, which appears to be another important manifestation of China’s environmental authoritarianism. This institutional setup severely restricts the ability of China’s environmental vulnerable groups to protect their rights and masks many environmental issues that truly need improvement, hindering the realization of environmental justice. From a comparative perspective, compared to countries like the United States with more mature experiences in environmental public interest litigation, China’s system suffers from narrow subject qualifications, extensive restrictions on environmental NGOs, and excessive litigation costs. Even compared to India, another developing country, China’s environmental public interest litigation system appears conservative. Therefore, China’s environmental public interest litigation system urgently needs further reform and improvement.
Clustering of spermatozoa examined through flow cytometry provides more information than the conventional assessment: a resilience to osmotic stress example
Authors: Valencia Julian , Bonilla-Correal Sebastián , Pinart Elisabeth , Bonet Sergi , Yeste Marc 
Published: May 2024

Context Conventional sperm quality tests may not be sufficient to predict the fertilising ability of a given ejaculate; thus, rapid, reliable and sensitive tests are necessary to measure sperm function. Aims This study sought to address whether a cluster analysis approach based on flow cytometry variables could provide more information about sperm function. Methods Spermatozoa were exposed to either isotonic (300 mOsm/kg) or hypotonic (180 mOsm/kg) media for 5 and 20 min, and were then stained with SYBR14 and propidium iodide (PI). Based on flow cytometry dot plots, spermatozoa were classified as either viable (SYBR14+/PI−) or with different degrees of plasma membrane alteration (SYBR14+/PI+ and SYBR14−/PI+). Moreover, individual values of electronic volume (EV), side scattering (SS), green (FL1) and red (FL3) fluorescence were recorded and used to classify sperm cells through cluster analysis. Two strategies of this approach were run. The first one was based on EV and the FL3/FL1 quotient, and the second was based on EV, SS and the FL3/FL1 quotient. Key results The two strategies led to the identification of more than three sperm populations. In the first strategy, EV did not differ between membrane-intact and membrane-damaged sperm, but it was significantly (P < 0.01) higher in spermatozoa losing membrane integrity. In the second strategy, three out of five subpopulations (SP2, SP3 and SP4) showed some degree of alteration in their plasma membrane with significant (P < 0.01) differences in EV. In both cluster analyses, SP5 (intact-membrane spermatozoa) presented the lowest EV. Besides, SP3 and SP4 (Strategy 1) and SP5 (Strategy 2) were found to be significantly (P < 0.05) correlated with sperm functional competence. Conclusions Cluster analysis based on flow cytometry variables provides more information about sperm function than conventional assessment does. Implications Combining flow cytometry with cluster analysis is a more robust approach for sperm evaluation.
Effect of gas pre-decomposition device on the growth of GaN epitaxial layer
Authors: Li Yazhou , Yao Weizhen , Ma Zhanhong , Yang Shaoyan , Liu Xianglin , Li Chengming , Wang Zhanguo 
Published: May 2024

Abstract
In previous studies, the influence of gas phase and surface reactions on the growth of GaN was mainly calculated through simulations. In this study, a novel gas pre-decomposition device (GPDD) was designed to experimentally investigate the effects of gas phase and surface reactions on GaN growth by changing the length and height of the isolation plates (IPs). By varying the structure of the GPDD, the effects on the growth rate and thickness uniformity of the GaN films were studied. The growth rate of the GaN sample slowed with the extension of the IPs because the longer partition plates led to insufficient gas mixing and premature consumption of the precursor trimethylgallium (TMG). The use of GPDD simultaneously achieves high crystal quality and smooth surface morphology of the GaN film. Owing to the use of GPDD, the decomposition of TMG in the pyrolysis pathway was promoted, which suppressed Ga vacancies and C impurities, resulting in weak yellow luminescence bands in the photoluminescence. This study provides a comprehensive understanding of the chemical reaction mechanism of GaN and plays an important role in promoting the development of metal-organic chemical vapor deposition equipment.
Superconductivity in Nb5Ir3N: a nitrogen-filled electride
Authors: Yang Qing-Song , Ruan Bin-Bin , Zhou Meng-Hu , Gu Ya-Dong , Zhao Kang , Shi Yun-Qing , Yi Jun-Kun , Chen Le-Wei , Ma Ming-Wei , Chen Gen-Fu , Ren Zhi-An 
Published: May 2024

Abstract
The hexagonal Mn5Si3-type compounds possess the capability to accommodate specific atoms in the interstices, thereby creating filled Mn5Si3-type structures. In Nb-based Mn5Si3-type system, interstitial atoms like carbon (C) or oxygen (O) have been identified to induce or enhance superconductivity. However, the compounds filled with nitrogen (N) are scarce, and the existence of a N-filled superconductor remains unknown. Here, we report the discovery of a novel ternary nitride superconductor, Nb5Ir3N, synthesized via incorporating N into the electride Nb5Ir3. The crystal structure of Nb5Ir3N conforms to the filled Mn5Si3-type, belonging to the P63/mcm space group (No. 193), with cell parameters a = b = 7.8398(2) Å and c = 5.1108(1) Å. Electrical resistivity and magnetic susceptibility demonstrate that Nb5Ir3N is a type-II superconductor with a T
c of 8.7 K. The estimated lower and upper critical fields are 11.0 mT and 12.16 T, respectively. Moreover, specific heat measurements confirm the bulk superconductivity with enhanced electron–phonon coupling in Nb5Ir3N, as demonstrated by the normalized specific heat jump ΔC
e/γT
c ∼ 1.59. First-principles calculations emphasize the strong spin–orbit coupling in Nb5Ir3N.
Scanning electrochemical probe microscopy investigation of two-dimensional materials
Authors: Adanigbo Pelumi , Romo-Jimenez Jorge , Zhang Kaidi , Maroo Sonal , Bediako Kwabena , Yu Yun 
Published: May 2024

Abstract
Research interests in two-dimensional (2D) materials have seen exponential growth owing to their unique and fascinating properties. The highly exposed lattice planes coupled with tunable electronic states of 2D materials have created manifold opportunities in the design of new platforms for energy conversion and sensing applications. Still, challenges in understanding the electrochemical (EC) characteristics of these materials arise from the complexity of both intrinsic and extrinsic heterogeneities that can obscure structure–activity correlations. Scanning EC probe microscopic investigations offer unique benefits in disclosing local EC reactivities at the nanoscale level that are otherwise inaccessible with macroscale methods. This review summarizes recent progress in applying techniques of scanning EC microscopy (SECM) and scanning EC cell microscopy (SECCM) to obtain distinctive insights into the fundamentals of 2D electrodes. We showcase the capabilities of EC microscopies in addressing the roles of defects, thickness, environments, strain, phase, stacking, and many other aspects in the heterogeneous electron transfer, ion transport, electrocatalysis, and photoelectrochemistry of representative 2D materials and their derivatives. Perspectives for the advantages, challenges, and future opportunities of scanning EC probe microscopy investigation of 2D structures are discussed.
Intraspecific variation in responses to extreme and moderate temperature stress in the wild species, Solanum carolinense (Solanaceae)
Authors: Chandler Emma K , Travers Steven E 
Published: May 2024

Abstract
Adaptation or acclimation to local temperature regimes has often been used as a proxy for predicting how plant populations will respond to impending novel conditions driven by human-caused climate change. To understand how plants may successfully respond to increasing air temperatures (extreme and moderate) in the future, we explored how temperature tolerance traits differ in populations of Solanum carolinense from northern (MN) and southern (TX) regions of the continental USA in a two-experiment study. In the first experiment, we compared the heat and cold tolerance in vegetative (sporophyte) and reproductive (male gametophyte) traits. In the second experiment, we studied if long-term heat influences plant development by examining how development in moderate heat affected reproductive structures and reproductive success. We found that temperature sensitivity differed between southern populations, which regularly experience extreme heat, and northern populations which do not. In contrast to our expectations, northern populations appeared more heat-tolerant than southern populations for vegetative traits such as chlorophyll stability and reproductive traits such as pollen germination. Our results are consistent with a heat-avoidance, rather than tolerance mechanism to mitigate extreme heat during pollen germination. In the second experiment, plants developing under the moderate heat treatment had significantly smaller reproductive structures and reduced seed production (27% fewer seeds on average than in the control treatment). Reproductive structures that developed in moderate heat were also reduced in size, particularly in the northern populations relative to populations from the south. We conclude that rising temperatures have the potential to incur substantial negative consequences for the reproductive success of individuals in this species and that some populations already mitigate stressful temperature conditions through phenotypic plasticity.
Two methods of isolation of rat aortic smooth muscle cells with high yield
Authors: Lotfollahzadeh Saran , Jose Asha , Zarnaab Shafiq Esha , El Sherif Nourhan , Smith Michael , Han Jingyan , Seta Francesca , Chitalia Vipul 
Published: May 2024

Abstract
Vascular smooth muscle cells (VSMCs) are an integral part of blood vessels and are the focus of intensive research in vascular biology, translational research, and cardiovascular diseases. Though immortalized vascular smooth muscle cell lines are available, their use is limited, underscoring the need for primary VSMCs. There are several methods for isolating primary cells from mice. However, the isolation method from rat blood vessels requires optimization, given the differences in the aorta of mice and rats. Here we compare two methods for VSMCs isolation from rats: enzymatic digestion and the “block” method. We observed a significantly higher yield of VSMCs using the enzymatic digestion method. We further confirmed that VSMCs expressed well-established VSMC-specific markers (calponin) with both methods and observed the persistence of this marker up to 9 passages, suggesting a continuation of the secretory phenotype of VSMCs. Overall, this work compares two methods and demonstrates a practical and effective method for isolating VSMCs from rat aorta, providing vascular biologists with a valuable and reliable experimental tool.
miR-125b-5p delivered by adipose-derived stem cell exosomes alleviates hypertrophic scarring by suppressing Smad2
Authors: Xu Chaolei , Zhang Hao , Yang Chen , Wang Ying , Wang Kejia , Wang Rui , Zhang Wei , Li Chao , Tian Chenyang , Han Chao , Li Mengyang , Liu Xu , Wang Yunwei , Li Yan , Zhang Jian , Li Yu , Luo Liang , Shang Yage , Zhang Lixia , Chen Yuxi , Shen Kuo , Hu Dahai 
Published: May 2024

Abstract

Background
Hypertrophic scarring is the most serious and unmet challenge following burn and trauma injury and often leads to pain, itching and even loss of function. However, the demand for ideal scar prevention and treatment is difficult to satisfy. We aimed to discover the effects and mechanisms of adipose-derived stem cell (ADSC) exosomes in hypertrophic scarring.

Methods
ADSC exosomes were isolated from the culture supernatant of ADSCs and identified by nanoparticle tracking analysis, transmission electron microscopy and western blotting. The effect of ADSC exosomes on wound healing and scar formation was detected by the wound model of BALB/c mice. We isolated myofibroblasts from hypertrophic scar tissue and detected the cell viability, proliferation and migration of myofibroblasts. In addition, collagen formation and fibrosis-related molecules were also detected. To further disclose the mechanism of ADSC exosomes on fibrosis in myofibroblasts, we detected the expression of Smad2 in hypertrophic scar tissue and normal skin and the regulatory mechanism of ADSC exosomes on Smad2. Injection of bleomycin was performed in male BALB/c mice to establish an in vivo fibrosis model while ADSC exosomes were administered to observe their protective effect. The tissue injury of mice was observed via hematoxylin and eosin and Masson staining and related testing.

Results
In this study, we found that ADSC exosomes could not only speed up wound healing and improve healing quality but also prevent scar formation. ADSC exosomes inhibited expression of fibrosis-related molecules such as α-smooth muscle actin, collagen I (COL1) and COL3 and inhibited the transdifferentiation of myofibroblasts. In addition, we verified that Smad2 is highly expressed in both hypertrophic scar tissue and hypertrophic fibroblasts, while ADSC exosomes downregulated the expression of Smad2 in hypertrophic fibroblasts. Further regulatory mechanism analysis revealed that microRNA-125b-5p (miR-125b-5p) is highly expressed in ADSC exosomes and binds to the 3′ untranslated region of Smad2, thus inhibiting its expression. In vivo experiments also revealed that ADSC exosomes could alleviate bleomycin-induced skin fibrosis and downregulate the expression of Smad2.

Conclusions
We found that ADSC exosomes could alleviate hypertrophic scars via the suppression of Smad2 by the specific delivery of miR-125b-5p.
Vernacular classicism and the Mausoleum of Halicarnassus: the architectural imaginary of a Danish interwar crematorium
Authors: Kristensen Troels Myrup 
Published: May 2024

Abstract
This article discusses a crematorium inaugurated in 1923 in the municipal Northern Cemetery (Nordre Kirkegård) of Aarhus as a case of vernacular classicism in interwar Denmark. While earlier works by its architect Sophus Frederik Kühnel (1851–1930) followed National Romantic, Renaissance, and Gothic models, the crematorium stands out because of its crowning feature, a stepped pyramid roof that emulates one of the most characteristic architectural elements of the mid-fourth-century BCE Mausoleum of Halicarnassus, described by Pliny the Elder (HN 36.31) and other ancient authors. This reference to one of the most famous funerary monuments from classical antiquity is both functionally and symbolically appropriate in a cemetery setting and also fits into a longer history of ‘Mausoleumania’, both in Denmark and internationally. Furthermore, it reflects a broader architectural movement of vernacular, ‘Nordic’ classicism during the interwar period, when local architects ‘translated’ classical elements into the vernacular, for example, through prominent use of brickwork and eclectic combinations of classical motifs with both medieval and National Romantic forms. Kühnel’s crematorium shows some of the creative ways in which Scandinavian architects freely adapted distinctive elements from the repertoire of classical architecture, in this case in response to the rise of cremation.
Analysis of meiotic recombination in Drosophila simulans shows no evidence of an interchromosomal effect
Authors: Man Bowen , Kim Elizabeth , Vadlakonda Alekhya , Stern David L , Crown K Nicole 
Published: May 2024

Abstract
Chromosome inversions are of unique importance in the evolution of genomes and species because when heterozygous with a standard arrangement chromosome, they suppress meiotic crossovers within the inversion. In Drosophila species, heterozygous inversions also cause the interchromosomal effect, whereby the presence of a heterozygous inversion induces a dramatic increase in crossover frequencies in the remainder of the genome within a single meiosis. To date, the interchromosomal effect has been studied exclusively in species that also have high frequencies of inversions in wild populations. We took advantage of a recently developed approach for generating inversions in Drosophila simulans, a species that does not have inversions in wild populations, to ask if there is an interchromosomal effect. We used the existing chromosome 3R balancer and generated a new chromosome 2L balancer to assay for the interchromosomal effect genetically and cytologically. We found no evidence of an interchromosomal effect in D. simulans. To gain insights into the underlying mechanistic reasons, we qualitatively analyzed the relationship between meiotic double-stranded break (DSB) formation and synaptonemal complex (SC) assembly. We found that the SC is assembled prior to DSB formation as in D. melanogaster; however, we show that the SC is assembled prior to localization of the oocyte determination factor Orb, whereas in D. melanogaster, SC formation does not begin until the Orb is localized. Together, our data show no evidence that heterozygous inversions in D. simulans induce an interchromosomal effect and that there are differences in the developmental programming of the early stages of meiosis.
Multi-agent reinforcement learning based algorithm detection of malware-infected nodes in IoT networks
Authors: Severt Marcos , Casado-Vara Roberto , del Rey Ángel Martín , Quintián Héctor , Luis Calvo-Rolle Jose 
Published: May 2024

Abstract
The Internet of Things (IoT) is a fast-growing technology that connects everyday devices to the Internet, enabling wireless, low-consumption and low-cost communication and data exchange. IoT has revolutionized the way devices interact with each other and the internet. The more devices become connected, the greater the risk of security breaches. There is currently a need for new approaches to algorithms that can detect malware regardless of the size of the network and that can adapt to dynamic changes in the network. Through the use of a multi-agent reinforcement learning algorithm, this paper proposes a novel algorithm for malware detection in IoT devices. The proposed algorithm is not strongly dependent on the size of the IoT network due to the that its training is adapted using time differences if the IoT network size is small or Monte Carlo otherwise. To validate the proposed algorithm in an environment as close to reality as possible, we proposed a scenario based on a real IoT network, where we tested different malware propagation models. Different simulations varying the number of agents and nodes in the IoT network have been developed. The result of these simulations proves the efficiency and adaptability of the proposed algorithm in detecting malware, regardless of the malware propagation model.
Development of an evaluation and treatment strategy for olfactory neuroblastoma: a review of evidence from large-scale studies, including population-based and multicenter studies, and meta-analyses
Authors: Ariizumi Yosuke , Asakage Takahiro 
Published: May 2024

Abstract
Olfactory neuroblastoma is a rare sinonasal malignancy arising from the olfactory epithelium that is characterized by skull base involvement and a modest natural history. Because of its rarity and long course, identification of independent prognostic factors is dependent on multivariate analysis of large, long-term data. In this review, we outline evidence for the evaluation and treatment of olfactory neuroblastoma obtained from recent large-scale population-based studies, meta-analyses and multicenter studies. Hyams grade is currently the only pathological grade system for olfactory neuroblastoma. The modified Kadish staging and Dulguerov classification are available for clinical staging. The results of large-scale studies have confirmed Hyams, the modified Kadish and Dulguerov as independent prognostic factors. Surgery followed by radiotherapy provides the best overall survival and recurrence-free survival for resectable disease. The question of whether postoperative radiotherapy should be administered for all cases or only for those at risk of recurrence remains unanswered. Exclusively endoscopic resection is indicated for modified Kadish A/B cases without any increase in the risk of death or recurrence, and is also indicated for modified Kadish C cases if a negative surgical margin is ensured. For more advanced cases, such as those with extensive brain infiltration, the open approach is indicated. Elective nodal irradiation prevents late nodal recurrence of N0 patients. Chemotherapy has failed to show a benefit in survival or disease control. Current needs for olfactory neuroblastoma include the development and validation of refined staging systems suitable for current practice; expansion of indications for endoscopic surgery; less invasive surgery; definitive radiotherapy and novel systemic therapy.
Erythrocyte membrane-camouflaged mesoporous silica composite nanoparticles for loading and controlled release of the hepatoprotective agent silibinin: A-synthesis and physicochemical characterization
Authors: Tayebi Khorrami Vahid , Raee Mohammad Javad , Abolmaali Samira Sadat , Abedanzadeh Mozhgan , Shafiee Mina , Tamaddon Ali Mohammad 
Published: May 2024

Abstract

Objectives
Milk thistle has long been used in the treatment of liver and biliary disorders. In the present study, to make a long-acting delivery system for silibinin (SBN, a major active constituent of milk thistle seeds with antioxidant and hepatoprotective function), mesoporous silica composite nanoparticles (NC) were synthesized and coated with RBC membrane.

Methods
A modified Stöber method was used for NC synthesis, which was then characterized using FE-SEM, DLS, TEM, FTIR, and EDAX techniques. A suitable lysis buffer was used to prepare RBC-ghost, and sonication was used to coat SBN-loaded NC (SBN-NC). The RBC-ghost coated SBN-NC (SBN-NC-RBCG) was evaluated by SDS-PAGE, Bradford, TEM, EDAX, and DLS methods. SBN release was then compared for the SBN-NC and SBN-NC-RBCG samples.

Key findings
the RBC membrane proteins were recovered from the coating of SBN-NC-RBCG, and SBN release was sustained over 24 h when compared with the SBN-NC.

Conclusions
Overall, through prolonging circulation in the bloodstream and evading the immune system, the developed system can improve SBN bioavailability in liver inflammation and fibrosis conditions that need further research.