bluuebunny/crossref_metadata_2025_split huggingface dataset:
Nanopore environmental DNA sequencing of catch water for estimating species composition in demersal bottom trawl fisheries
Authors: Maggini Sara , Jacobsen Magnus Wulff , Urban Paulina , Hansen Brian Klitgaard , Kielgast Jos , Bekkevold Dorte , Jardim Ernesto , Martinsohn Jann T. , Carvalho Gary R. , Nielsen Einar E. , Papadopulos Alexander S. T. 
Published: May 2024

AbstractBycatch and discards, representing unwanted catches, undermine sustainable fisheries and hinder the conservation of vulnerable and endangered species. To effectively monitor bycatch and enhance the effectiveness of management measures while promoting sustainable fishing practices, reliable data is essential. Here, we explore the use of Nanopore metabarcoding to analyze the catch composition in demersal bottom fisheries. We collected eDNA samples directly from an onboard catch holding tank (catch water) for 10 fishing hauls from a fishing vessel operating in the Skagerrak (North‐East Atlantic). The approach involved sequencing a combination of long (~2 kb) and short (~170–313 bp) mitochondrial amplicons and was validated by analyzing a fishery‐related mock community sample and fishing haul replicates. Overall, the detection rate accuracy was 95% for landed species, and replicates obtained from the same fishing haul showed consistent results, validating the robustness of this approach. The detection rate accuracy for all caught species observed on board (including the non‐landed fraction) was 81%. Undetected species were always limited to species in low abundance, but may also be attributed to problems with identifying closely related species due to the impact of sequencing errors and limited diagnostic variation in the genetic regions used. In the future, such biases may be reduced by using additional markers to increase species discrimination power and applying newly available technological advantages in flow cell chemistry to improve sequencing accuracy. In conclusion, this study demonstrates the effectiveness of Nanopore eDNA sequencing of catch water for estimating species composition in demersal bottom trawl fisheries, including catches of non‐commercial and threatened and vulnerable species, without disrupting fishing activities. Incorporating eDNA analysis of catch water may therefore help facilitate effective monitoring, leading to better‐informed fisheries management, biodiversity conservation efforts, and the implementation of relevant legislation such as the EU landing obligation.
Environmental DNA storage and extraction method affects detectability for multiple aquatic invasive species
Authors: García Samantha M. , Chun Chan Lan , Dumke Josh , Hansen Gretchen J. A. , Quebedeaux Kathleen B. , Rounds Christopher , Totsch Anna , Larson Eric R. 
Published: May 2024

AbstractEnvironmental DNA (eDNA) refers to genetic material released by organisms into their surrounding environment. Collecting and identifying eDNA has gained popularity for monitoring and surveillance of aquatic invasive species. Invasive species management is most successful when an invasion is identified early while population size is likely to be low, highlighting the importance of eDNA detection sensitivity. Various factors influence DNA yield recovered from environmental samples. Environmental DNA storage and extraction methods, for example, can be adjusted to maximize DNA yield, thereby improving detectability. In this study, we compared the performance of two eDNA storage and extraction methods in detecting three common aquatic invasive species (Bythotrephes longimanus, Dreissena polymorpha, and Faxonius rusticus) across five natural ecosystems of Minnesota, United States. One method involved storing filters in 95% ethanol (EtOH) and extracting DNA using a DNeasy PowerSoil Pro Kit (Qiagen, Hilden, Germany), whereas the other method used cetyl trimethylammonium bromide (CTAB) for storage and a phenol–chloroform–isoamyl (PCI) procedure for DNA extraction. We also investigated the effect of DNA extract volume (1 μL relative to 3 μL) in qPCR reactions on eDNA detections for the commercial kit method. The CTAB‐PCI method yielded significantly more positive detections, across all three species, compared to the EtOH‐Qiagen method. Moreover, we found that using 1 μL of DNA extract in qPCR reactions was equally effective as using 3 μL. To improve detections of aquatic invasive species, we recommend that researchers store eDNA sample filters in CTAB or a similar lysis buffer such as Longmire's solution and extract with PCI when feasible, but note that lower extract volumes might be used without negative effect when either increasing technical replicates or repurposing samples for the detection of multiple species.
Application of microbial fuel cell‐based biosensor in environmental monitoring – A critical review
Authors: Liu Cheng , Cheng Liang , Jia Hui 
Published: May 2024

AbstractMicrobial Fuel Cells (MFCs) represent an innovative approach for transforming biomass energy directly into electricity, which showed great promise in various applications beyond energy generation and wastewater treatment. The use of MFCs as biosensors for in‐situ and online monitoring has garnered increasing interest. These biosensors stand out for their compactness, ease of operation, affordability, and portability. They have proven effectively in the detection of various water quality indicators, including organic matter, nitrogen, heavy metals, pH levels, and dissolved oxygen. This comprehensive review aims to provide a critical analysis of the current research landscape and the latest advancements in MFC technology, with special emphasis on the challenges encountered in its application for wastewater and water quality monitoring. Moreover, strategies for performance improvement, such as the adoption of miniaturized structures, the exploration of innovative materials, and the application of mathematical modelling for analysis, are also discussed. The review also explores potential avenues for future research, especially in the realm of detecting mixed pollutants. Thus, it provides insightful perspectives on the evolving field of biosensor technology based on MFCs.
Sugarcane leaf return and mixing significantly reduce slope runoff and soil loss
Authors: Lin Zhe , Deng Yusong , Li Juhui , Yang Yuanfeng , Wang Jianyu , Yang Gairen , Duan Xiaoqian 
Published: May 2024

AbstractImproper management of sugarcane cultivation may cause severe soil erosion, especially in lateritic red soil hilly areas. Crop straw return to the field can effectively improve the soil structure, but there is a lack of evaluation studies of the soil and water conservation benefits of sugarcane leaf mixing and return to the field, and the underlying regulatory mechanism has rarely been described. Thus, we conducted a series of simulated rainfall experiments to investigate the impact of returning different mixed sugarcane leaf weights (0, 3, 6, 9, 12 and 15 t/ha) to the field on erodible lateritic red soil slopes that support sugarcane production under various slopes (5°, 10°, 15° and 20°) in different rainfall events (60 and 120 mm/h). The results indicated that returning sugarcane leaves into farmland could effectively regulate the process of runoff and sediment production on lateritic red soil slopes, and the optimal mixing amount was 9 t/ha. Under a low rainfall intensity, the erosion process was mainly affected by the slope, and the benefits of applying the sugarcane leaf mixing treatment to the slope to reduce runoff and sediment could reach 13.37–53.04% and 23.86–77.55%, respectively. However, under a high rainfall intensity, the sugarcane leaf mixing amount was the main influencing factor, the benefits for reducing runoff and sediment could reach 4.64–38.72% and 30.87–93.14%, respectively. The sugarcane leaf mixing amount exhibited a linear relationship with the Reynolds number (R2 > 0.63), whereas the runoff velocity (R2 > 0.96), Froude number (R2 > 0.94) and drag coefficient (R2 > 0.85) exhibited well‐fitting exponential function relationship with it. This study provides a reference for future endeavours regarding the inhibition mechanisms of sugarcane leaves mixed and returned to the field for decreasing slope erosion.
Enhanced authentication and device integrity protection for GDOI using blockchain
Authors: Mukhandi Munkenyi , Andrade Eduardo , Granjal Jorge , Vilela João P. 
Published: May 2024

AbstractRecent device‐level cyber‐attacks have targeted IoT critical applications in power distribution systems integrated with the Internet communications infrastructure. These systems utilize group domain of interpretation (GDOI) as designated by International Electrotechnical Commission (IEC) power utility standards IEC 61850 and IEC 62351. However, GDOI cannot protect against novel threats, such as IoT device‐level attacks that can modify device firmware and configuration files to create command and control malicious communication. As a consequence, the attacks can compromise substations with potentially catastrophic consequences. With this in mind, this article proposes a permissioned/private blockchain‐based authentication framework that provides a solution to current security threats such as the IoT device‐level attacks. Our work improves the GDOI protocol applied in critical IoT applications by achieving decentralized and distributed device authentication. The security of our proposal is demonstrated against known attacks as well as through formal mechanisms via the joint use of the AVISPA and SPAN tools. The proposed approach adds negligible authentication latency, thus ensuring appropriate scalability as the number of nodes increases.
Kumquat pomace removal of free polyphenol alleviates induced acute enteritis and restores gut microbiota in dextran sodium sulphate‐treated mice
Authors: Ji Chunxiao , Huang Yixin , Xie Wenjie , Zhu Yawen , Qin Jingping , Guo Shiyin , Xiao Hang , Fan Wei , Tang Zhonghai 
Published: May 2024

AbstractKumquat pomace non‐extractable polyphenols (KNEP), which contains celobiotics and has anti‐inflammatory and antioxidant effects, are one of the main components in kumquats. We aimed to determine the preventive and inhibitory effects of KNEP on dextran sodium sulphate (DSS)‐induced inflammatory bowel disease (IBD). The disease activity index (DAI) score, H&E staining, high‐throughput sequencing technology, quantitative analysis, ELISA, and western blotting were used to explore the effect of KNEP in an IBD mouse model. The DAI score and H&E staining results showed that compared with the DSS treatment group, the DAI and pathological status of IBD in the DSS + 3% KNEP (low‐dose) and DSS + 6% KNEP (high‐dose) intervention groups were significantly improved. High‐throughput sequencing showed that the composition of the intestinal flora was restored under KNEP, and the abundance of pathogenic bacteria decreased and that of beneficial bacteria increased. Quantitative analysis of short‐chain fatty acids (SCFAs) showed that the SCFAs content in the cecum of IBD mice increased in a dose‐dependent manner. ELISA results showed that KNEP at different doses could inhibit the expression of TNF‐α, IL‐1β, IFN‐γ, and other inflammatory factors, and that 6% KNEP had the most potent inhibitory effect. Western blotting results showed that KNEP inhibited the expression of TLR4, NF‐κB, and NLRP3 in the colon of mice with DSS‐induced colitis in a dose‐dependent manner. These results indicate that KNEP can significantly improve DSS‐induced acute colitis in mice, and the higher the KNEP content, the more pronounced the improvement in IBD.
SAN: Mitigating spatial covariance heterogeneity in cortical thickness data collected from multiple scanners or sites
Authors: Zhang Rongqian , Chen Linxi , Oliver Lindsay D. , Voineskos Aristotle N. , Park Jun Young 
Published: May 2024

AbstractIn neuroimaging studies, combining data collected from multiple study sites or scanners is becoming common to increase the reproducibility of scientific discoveries. At the same time, unwanted variations arise by using different scanners (inter‐scanner biases), which need to be corrected before downstream analyses to facilitate replicable research and prevent spurious findings. While statistical harmonization methods such as ComBat have become popular in mitigating inter‐scanner biases in neuroimaging, recent methodological advances have shown that harmonizing heterogeneous covariances results in higher data quality. In vertex‐level cortical thickness data, heterogeneity in spatial autocorrelation is a critical factor that affects covariance heterogeneity. Our work proposes a new statistical harmonization method called spatial autocorrelation normalization (SAN) that preserves homogeneous covariance vertex‐level cortical thickness data across different scanners. We use an explicit Gaussian process to characterize scanner‐invariant and scanner‐specific variations to reconstruct spatially homogeneous data across scanners. SAN is computationally feasible, and it easily allows the integration of existing harmonization methods. We demonstrate the utility of the proposed method using cortical thickness data from the Social Processes Initiative in the Neurobiology of the Schizophrenia(s) (SPINS) study. SAN is publicly available as an R package.
Diagnostic value of 18F‐FDG PET/CT versus diffusion‐weighted MRI in detection of residual or recurrent tumors after definitive (chemo) radiotherapy for laryngeal and hypopharyngeal squamous cell carcinoma: A prospective study
Authors: Kim Soung Yung , Crook David , Rosskopf Johannes , Lee Jung‐Hyun 
Published: May 2024

AbstractBackgroundDespite advances in treatment, residual or recurrent tumors after definitive (chemo) radiotherapy for laryngeal and hypopharyngeal squamous cell carcinoma (SCC) remain a challenge in clinical management and require accurate and timely detection for optimal salvage therapy. This study aimed to compare the diagnostic value of Fluorine 18 (18F) fluorodeoxyglucose (FDG) positron emission tomography (PET)/computed tomography (CT) and diffusion‐weighted magnetic resonance imaging (DW‐MRI) in detecting residual or recurrent tumors after definitive (chemo) radiotherapy for laryngeal and hypopharyngeal SCC.MethodsA prospective study was conducted on 30 patients who presented with new symptoms after definitive (chemo) radiotherapy for laryngeal (n = 21) and hypopharyngeal (n = 9) carcinoma. Both 18F‐FDG PET/CT and DW‐MRI were performed and histopathologic analysis served as the standard of reference.ResultsHistopathology showed 20 patients as positive and 10 as negative for tumors. 18F‐FDG PET/CT detected all tumors correctly but was falsely positive in one case. DW‐MRI detected tumors in 18 out of 20 positive patients and correctly excluded tumors in all negative patients. The sensitivity and specificity of 18F‐FDG PET/CT were 100% and 90%, respectively, while the values for DW‐MRI were 90% and 100%, respectively.ConclusionsThe study concludes that 18F‐FDG PET/CT is slightly superior to DW‐MRI in detecting residual or recurrent tumors after definitive (chemo) radiotherapy for laryngeal and hypopharyngeal SCC. The combined use of 18F‐FDG PET/CT and DW‐MRI can potentially improve specificity in therapy response evaluation.
Support for a browser plug‐in blocking online alcohol imagery among Australian participants: A qualitative interview study
Authors: Patsouras Maree , Cook Megan , Pennay Amy , Kuntsche Emmanuel , Manning Victoria , He Zhen , Riordan Benjamin C. 
Published: May 2024

AbstractIssues AddressedAlcohol depictions are extremely common online, and there is a reported relationship between alcohol exposure and alcohol use. A browser plug‐in specifically designed to block online alcohol depictions may be helpful to prevent the uptake of alcohol or increased alcohol use.MethodsTwenty‐five semi‐structured interviews were conducted, examining participants' beliefs about the effects of online alcohol exposure and their support for developing the browser plug‐in.ResultsUsing reflexive thematic analysis, we found participants highlighted a clear impact of viewing alcohol online and offline alcohol use. Participants believed a browser plug‐in that blocked alcohol was acceptable and would be especially useful for minors (to prevent alcohol initiation) and those who are aiming to reduce their alcohol use.ConclusionsParticipants emphasised that viewing online alcohol exposure had an impact on drinking behaviours, such as increased craving and temptation. The browser plug‐in was considered an easy intervention tool for both parents and people who are experiencing alcohol‐related problems or trying to reduce their drinking.So What?Participants' continuous support of an alcohol‐blocking browser plug‐in suggests that future health promotion strategies should consider the development of a prototype plug‐in.
A decadal analysis of road traffic accident‐related mortality among women of reproductive age: A cross‐sectional study in East Azerbaijan Province, Iran
Authors: Golestani Mina , Sadeghi‐Bazargani Homayoun , Gavgani Leili Faraji , Khabiri Roghayeh , Jahangiry Leila 
Published: May 2024

AbstractBackgroundGiven the extensive impact of road traffic accidents (RTAs) consequences and their potential ramifications on the health of both current and future generations, this study examines the social and demographic factors that influence RTA‐related mortality among women of reproductive age.MethodsThe study population consisted of cases retrieved from the database of the Legal Medicine Organization, encompassing all women aged 15–49 who succumbed to road accidents between 2011 and 2021.ResultsThe mean age of women in the reproductive age group from East Azerbaijan province between 2011 and 2021 was 33.67 years, with a standard deviation of 9.18. RTAs on main roads accounted for the majority of incidents (395 cases, 50.8%), with 93.7% (728 cases) attributed to road traffic. In 54.4% of these cases, the affected organ was the head and neck, and the primary cause of death in 52.1% was head trauma. Across all age groups, injuries to the neck and head were the most common, followed by injuries to the abdomen, chest, back, and sternum.ConclusionThe higher incidence of road accidents in the 25–29 age group highlights the need for targeted interventions to address risky behaviors, inexperience, and peer influences in this demographic. Our observation of passengers experiencing the highest mortality rate emphasizes the vulnerability of road users, particularly pedestrians, in traffic accidents. Pedestrian violations in the 17–30 age group further emphasize the importance of education and awareness campaigns aimed at reducing risky behavior.
The pathogenicity and potential risk evaluation of Zika virus to cause mysterious “Disease X”: A brief report
Authors: Tushar Md. Ismail , Islam Md. Rabiul 
Published: May 2024

AbstractBackground and AimsDisease X is included to represent an unknown pathogen that has the potential to spread globally and trigger significant epidemics. The World Health Organization (WHO) and the Global Alliance for Vaccine and Immunization (GAVI) recommend 10 potential disease organisms responsible for disease X. Among them, the Zika virus (ZIKV) is one of the potential organisms. The present work aimed to evaluate the current pathogenicity and potential risk of the ZIKV for public health emergencies.MethodsWe performed a brief review of existing literature from available sources using Zika fever, Zika virus, and Flaviviridae as keywords and extracted relevant information for this review.ResultsThe primary way that the ZIKV transmits to humans is by mosquito bites (Aedes aegypti and Aedes albopictus). Typically, Aedes mosquitoes bite during the day. As an RNA virus, the ZIKV is well‐known for its rapid mutation rate. ZIKV strains or variations can arise due to genomic mutations, and they occur through various processes, including replication errors, recombination, and selection pressure. Nowadays, the ZIKV has also evolved into a global pandemic because the four‐amino acid sequence fits the envelope protein's 154 glycosylation motif, which may be involved in virulence.ConclusionThere is no specific medicine or vaccine available for the prevention or treatment of ZIKV infection. Therefore, personal protective measures should be the way to prevent ZIKV disease. Therefore, avoiding exposure is the best way to prevent problems from ZIKV to mosquitoes.
The association of sociodemographic factors and history of chronic diseases on menopausal symptoms: A cross‐sectional study
Authors: Sharami Seyedeh Hajar , Rafiei Sorouri Zahra , Farzadi Sara , Hosseinzadeh Fatemeh , Etezadi Atoosa , Bab Eghbal Sedighe , Eslami‐Kenarsari Habib 
Published: May 2024

AbstractBackgroundMenopausal symptoms are very diverse in terms of prevalence and severity, and this difference is due to various factors such as psychological factors, sociocultural status, lifestyle, geographical location, and other factors. This study aimed to assess the prevalence of menopausal symptoms and evaluate the predictive factors related to the prevalence and severity of menopausal symptoms.Materials and MethodsThis was a cross‐sectional analytical study that was performed on 214 women aged 35–65 years old who were referred to Alzahra Educational, Research and Treatment Center in Rasht, Iran. The data collection tool was a valid and reliable questionnaire, using the list of menopausal symptoms and a checklist of subjects’ general characteristics.Results16.8% of postmenopausal women in our study had at least one menopausal symptom. Using multiple linear regression, race (p = 0.02), history of chronic diseases (p = 0.04), place of residence (p = 0.02), and marital satisfaction (p = 0.02) were associated with menopausal symptoms. Nineteen percent of the covariates related to the logistics function were explained by the predictor variables in the model.ConclusionEvaluation of menopausal symptoms showed that the severity of menopausal symptoms was related to factors such as body mass index (BMI), ethnicity, place of residence, marital satisfaction, and history of chronic diseases, and need to address BMI, psychological issues, and chronic illness.
Thermohydraulic performance augmentation of triangular duct solar air heater using semi‐conical vortex generators: Numerical and experimental study
Authors: Pramod G. K. , Madhwesh N. , Arunachala U. C. , Manjunath M. S. 
Published: May 2024

AbstractThermohydraulic performance augmentation using turbulence promotors is a commonly adopted technique in solar air heater (SAH) applications. This article presents the thermohydraulic performance augmentation of triangular duct SAH using semi‐conical vortex generators (SCVG) using computational fluid dynamics and experimental methodology for various flow Reynolds numbers ranging from 6000 to 21,000. An in‐depth parametric analysis is undertaken to establish the influence of flow attack angle, relative longitudinal pitch, relative transverse pitch and cone diameter of SCVG on the thermohydraulic performance as indicated by the thermohydraulic performance parameter (THPP). The results reveal that the SCVG generates longitudinal vortices and introduces flow impingement zones which significantly affects the flow and heat transfer characteristics of air heaters. Correlations for Nusselt number and friction factor are established, which predicts the performance outcomes with an average error of 6.74% and 4.46%, respectively. The optimal THPP is determined to be 1.74 using artificial neural network model and Bonobo Optimization algorithm. The SCVG produces THPP values well above unity for the entire flow Reynolds number range of 6000–21,000.