bluuebunny/crossref_metadata_2025_split huggingface dataset:
TNF‐α can promote membrane invasion by activating the MAPK/MMP9 signaling pathway through autocrine in bone‐invasive pituitary adenoma
Authors: Wu Xinzhi , Gong Lei , Li Bin , Bai Jiwei , Li Chuzhong , Zhang Yazhuo , Zhu Haibo 
Published: May 2024

AbstractAimsA bone‐invasive pituitary adenoma exhibits aggressive behavior, leading to a worse prognosis. We have found that TNF‐α promotes bone invasion by facilitating the differentiation of osteoclasts, however, before bone‐invasive pituitary adenoma invades bone tissue, it needs to penetrate the dura mater, and this mechanism is not yet clear.MethodsWe performed transcriptome microarrays on specimens of bone‐invasive pituitary adenomas (BIPAs) and noninvasive pituitary adenomas (NIPAs) and conducted differential expressed gene analysis and enrichment analysis. We altered the expression of TNF‐α through plasmids, then validated the effects of TNF‐α on GH3 cells and verified the efficacy of the TNF‐α inhibitor SPD304. Finally, the effects of TNF‐α were validated in in vivo experiments.ResultsPathway act work showed that the MAPK pathway was significantly implicated in the pathway network. The expression of TNF‐α, MMP9, and p‐p38 is higher in BIPAs than in NIPAs. Overexpression of TNF‐α elevated the expression of MAPK pathway proteins and MMP9 in GH3 cells, as well as promoted proliferation, migration, and invasion of GH3 cells. Flow cytometry indicated that TNF‐α overexpression increased the G2 phase ratio in GH3 cells and inhibited apoptosis. The expression of MMP9 was reduced after blocking the P38 MAPK pathway; overexpression of MMP9 promoted invasion of GH3 cells. In vivo experiments confirm that the TNF‐α overexpression group has larger tumor volumes. SPD304 was able to suppress the effects caused by TNF‐α overexpression.ConclusionBone‐invasive pituitary adenoma secretes higher levels of TNF‐α, which then acts on itself in an autocrine manner, activating the MAPK pathway and promoting the expression of MMP9, thereby accelerating the membrane invasion process. SPD304 significantly inhibits the effect of TNF‐α and may be applied in the clinical treatment of bone‐invasive pituitary adenoma.
New cash cropping in the Black Volta river valley: Banana production, rural innovation, and social entrepreneurship in the Ghana–Burkina Faso border region
Authors: Lobnibe Isidore , Lobnibe Jane‐Frances Yirdong 
Published: May 2024

AbstractDuring the mid‐1990s, a banana river irrigation farm that was launched in Southwestern Burkina Faso by a returning emigrant refugee spread throughout the region as far as neighboring Ghana. With relative abundant fertile, arable farmland in Ghana, easy access to input and modest capital mobilization by a few Ghanaian University lecturers to clear farmland and construct ridges and lay pipes for irrigation, a new cash crop production was set in motion. This paper analyzes the implication of this innovative rural agricultural intensification in the Ghana side of the border by shifting the angle of analysis away from narratives of transnational African agricultural commercialization driven by foreign corporations to instead focus on the role played by the local elite. The paper demonstrates that the viability of this irrigated farming can be attributed to imported banana crop variety, local rural producers' entrepreneurial zest, and enhanced existing cross‐border ties and commercial opportunities made possible by the construction of a strategic regional highway linking the farm sites with large urban and market centers in Burkina Faso.
Identifying the deficiencies of currently available CGM to improve uptake and benefit
Authors: Barnard‐Kelly Katharine D. , Martínez‐Brocca Maria Asunción , Glatzer Timor , Oliver Nick 
Published: May 2024

AbstractBackground and AimsThe use of diabetes technologies is increasing worldwide, with health systems facilitating improved access to devices. Continuous glucose monitoring is a complex intervention that provides information on glucose concentration, rate and direction of change, historical data and alerts and alarms for extremes of glucose. These data do not themselves change glycaemia and require translation to a meaningful action for impact. It is, therefore, crucial that such systems advance to better meet the needs of individuals using them.MethodsNarrative review of the use of, engagement with, limitations and unmet needs of continuous glucose monitoring systems.ResultsCGM devices have made a significant contribution to the self‐management of diabetes; however, challenges with access and user experience persist, with multiple limitations to uptake and benefit. These limitations include physical size and implementation, with associated stigma, alarm fatigue, sleep disturbance and the challenge of addressing large volumes of real‐time data. Greater personalisation throughout the continuous glucose monitoring journey, with a focus on usability, may improve the benefits derived from the device and reduce the burden of self‐management. Healthcare professionals may have unconscious biases that affect the provision of continuous glucose monitors due to deprivation, education, age, ethnicity and other characteristics.ConclusionsContinuous glucose monitoring exerts a dose‐dependent response; the more it is used, the more effective it is. For optimal use, continuous glucose monitors must not just reduce the burden of management in one dimension but facilitate net improvement in all domains of self‐management for all users.
Development of an International Standard Set of Outcomes and Measurement Methods for Routine Practice for Adults with Epilepsy: The International Consortium for Health Outcomes Measurement Consensus Recommendations
Authors: Mitchell James W. , Sossi Frieda , Miller Isabel , Jaber Paula Blancarte , Das‐Gupta Zofia , Fialho Luz Sousa , Amos Action , Austin Joan K. , Badzik Scott , Baker Gus , Zeev Bruria Ben , Bolton Jeffrey , Chaplin John E. , Cross J. Helen , Chan Derrick , Gericke Christian A. , Husain Aatif M. , Lally Lorraine , Mbugua Sharon , Megan Cassidy , Mesa Tomás , Nuñez Lilia , von Oertzen Tim J. , Perucca Emilio , Pullen Angie , Ronen Gabriel M. , Sajatovic Martha , Singh Mamta B. , Wilmshurst Jo M. , Wollscheid Leonie , Berg Anne T. 
Published: May 2024

AbstractAt present, there is no internationally accepted set of core outcomes or measurement methods for epilepsy clinical practice. Therefore, the International Consortium for Health Outcomes Measurement (ICHOM) convened an international working group of experts in epilepsy, people with epilepsy and their representatives to develop minimum sets of standardized outcomes and outcomes measurement methods for clinical practice that support patient–clinician decision‐making and quality improvement. Consensus methods identified 20 core outcomes. Measurement tools were recommended based on their evidence of strong clinical measurement properties, feasibility, and cross‐cultural applicability. The essential outcomes included many non‐seizure outcomes: anxiety, depression, suicidality, memory and attention, sleep quality, functional status, and the social impact of epilepsy. The proposed set will facilitate the implementation of the use of patient‐centered outcomes in daily practice, ensuring holistic care. They also encourage harmonization of outcome measurement, and if widely implemented should reduce the heterogeneity of outcome measurement, accelerate comparative research, and facilitate quality improvement efforts.
Panicum maximum cultivars for use in integrated agricultural production systems in Cerrado biome soils
Authors: Bublitz Lucas Ruaro , Gurgel Antonio Leandro Chaves , Mauri Alexandre César , Queiroz Vitor Cardoso , de Souza Lima Kermilly , Campelo Itália Bianca Reis , de Araújo Marcos Jácome , Dias‐Silva Tairon Pannunzio , da Silva Barros Juliana , Aguiar Isadora Osório Maciel , dos Santos Difante Gelson , Ítavo Luís Carlos Vinhas 
Published: May 2024

AbstractIntegrated production systems have been an alternative for diversifying agricultural production, and therefore, it is necessary to study tropical forage grasses that can impact both straw production and soil organic matter, as well as benefit animal production. The objective of this work was to evaluate the productive characteristics and accumulation of nutrients in the biomass of grasses of the genus Panicum grown in the off‐season, in Cerrado biome soil as cover plants for use in integrated production systems. It evaluated different grasses of Panicum maximum: Aruana, Tamani, and Massai guinea grasses, distributed in a randomized block design with four replications of each grass. The productive characteristics, mineral accumulation, and carbon/nitrogen (C/N) ratio in the biomass of the cultivars were evaluated. No cultivar effect was observed for forage mass (3997.4 kg/ha DM). However, the largest (p = 0.0077) leaf blade masses were observed in the Massai and Tamani guinea grasses. On the contrary, the Aruana guinea grass exhibited the highest value (p = 0.0001) for stem mass, reflected in a higher (p = 0.0001) leaf/stalk ratio in the Massai and Tamani guinea grasses. No effect (p > 0.05) of the cultivar on micronutrients, magnesium, and phosphorus concentrations was observed. However, the Aruana guinea grass presented higher concentrations of calcium and sulfur; on the other hand, potassium values were higher in the Massai and Tamani guinea grasses. The Massai guinea grass exhibited a higher (p = 0.0214) C/N ratio, while the Aruana guinea grass recorded lower values, with no significant differences between these cultivars and Tamani guinea grass. The Tamani and Massai guinea grasses stand out as recommended choices for cultivation during the off‐season in Cerrado biome soils as cover crops in integrated production systems.
RAZOR‐THIN MASS ELECTIONS WITH HIGH TURNOUT
Authors: Levine David K. , Martinelli Cesar 
Published: May 2024

AbstractWe argue that traditional voting models fail to fully explain the frequency of very close mass elections with high turnout. Instead, we model elections as a competition between incentive schemes to mobilize voters. We elucidate conditions under which parties might prefer close elections, as the potential to be pivotal motivates voters instead of exclusively costly incentives as in nonclose elections. We show that, under those conditions, better voter targeting results in tighter races and increased turnout. Furthermore, the smaller party often has a strong incentive to commit to strategies that ensure a close election.
Structural, dielectric, electrical, and energy storage properties of Mn‐doped Ba0.55Sr0.45TiO3 ceramics
Authors: Ullah Ihsan , Rehman Maqbool Ur , Manan Abdul , Lanagan Michael T. , Khan Raj Wali , Ullah Atta , Ullah Shah Wali , Uzair Muhammad 
Published: May 2024

AbstractA conventional solid‐state reaction route was utilized for the fabrication of Ba0.55Sr0.45Ti1−xMnxO3 (x = .004, .006, .008, .01, and .015) ceramics. X‐ray diffraction (XRD) diffractograms revealed pseudo‐cubic structural symmetry with a single phase. The scanning electron microscope (SEM) images revealed fine grain morphology for x = .006, while an obvious increase in grain size was detected at x > .006. A high‐energy storage density (Ws) of 2.47 J cm−3 and a recoverable energy density (Wrec) of 1.36 J cm−3 at an applied electric field of 220 kV cm−1 were achieved for x = .006. An impedance spectroscopic study showed the electrical response relationship with microstructure. The observed two semicircles in Nyquist plots are an indication of the contribution of grains (bulk) and grain boundaries as a resistive medium for conduction of charge carriers, which led to enhanced the capability in capacitor applications.
The importance of the simulated wound fluid composition and properties in the determination of the fluid handling performance of wound dressings
Authors: Svensby Anna U. , Nygren Erik , Gefen Amit , Cullen Breda , Ronkvist Åsa M. , Gergely AnnBritt , Craig Marina D. 
Published: May 2024

AbstractEffective fluid handling by wound dressings is crucial in the management of exuding wounds through maintaining a clean, moist environment, facilitating healing by removing excess exudate and promoting tissue regeneration. In this context, the availability of reliable and clinically relevant standardised testing methods for wound dressings are critical for informed decision making by clinicians, healthcare administrators, regulatory/reimbursement bodies and product developers. The widely used standard EN 13726 specifies the use of Solution A, an aqueous protein‐free salt solution, for determining fluid‐handling capacity (FHC). However, a simulated wound fluid (SWF) with a more complex composition, resembling the protein, salt, and buffer concentrations found in real‐world clinical exudate, would provide a more clinically relevant dressing performance assessment. This study compared selected physicochemical parameters of Solution A, an alternative, novel simulated wound fluid (SWF A), and a benchmark reference serum‐containing solution (SCS) simulating chronic wound exudate. Additionally, FHC values for eight advanced bordered and non‐bordered foam dressings were determined for all three test fluids, following EN 13726. Our findings demonstrate a close resemblance between SWF A and SCS. This study highlights the critical importance of selecting a physiochemically appropriate test fluid for accurate FHC testing resulting in clinically meaningful evaluation of dressing performance.
Positive and negative social media experiences and proximal risk for suicidal ideation in adolescents
Authors: Hamilton Jessica L. , Dalack Maya , Boyd Simone Imani , Jorgensen Saskia , Dreier Melissa J. , Sarna Jas , Brent David A. 
Published: May 2024

BackgroundSocial media (SM) has received considerable attention as a potential risk factor for adolescent suicide. Few empirical studies, however, have examined adolescents' daily negative and positive experiences on SM and its proximal impacts on suicidal ideation (SI), particularly using intensive monitoring designs.MethodAdolescents (N = 60; 14–17 years; 49% girls; 62% LGBTQ+) recruited using SM across the United States and participated in an 8‐week intensive monitoring protocol. Ecological momentary assessment (three brief surveys per day) asked about negative and positive SM experiences and SI (passive and active). Multilevel modelling was used to evaluate the within‐person relationships between daily SM experiences (e.g. individual fluctuations compared to a person's average) and SI, controlling for average levels of SM experiences, SM use screen time, and lifetime SI.ResultsSignificant within‐person effects of negative and positive SM experiences were associated with days when adolescents had SI. Specifically, on days when teens endorsed more frequent negative SM experiences than usual, they were more likely to report SI. However, more positive SM experiences than usual were associated with a lower likelihood of having SI. There were no significant effects of SM use (‘screen time’) on SI or on the reverse associations of SI on next‐day SM experiences.ConclusionsResults indicate that SM experiences may be dynamic and modifiable risk and protective factors for SI in adolescents, whereas there is no effect of SM screen time on SI. Our results highlight that targeting negative SM experiences and augmenting the positive experiences on SM may be critical targets to improve teens' mental health and prevent suicide, rather than focusing on limiting SM screen time.
Melatonin ameliorates 10‐hydroxycamptothecin‐induced oxidative stress and apoptosis via autophagy‐regulated p62/Keap1/Nrf2 pathway in mouse testicular cells
Authors: Cheng Jinmei , Xu Junjie , Gu Yimin , Wang Yueming , Wang Jianyu , Sun Fei 
Published: May 2024

Abstract10‐Hydroxycamptothecin (HCPT) is a widely used clinical anticancer drug but has a significant side effect profile. Melatonin has a beneficial impact on the chemotherapy of different cancer cells and reproductive processes, but the effect and underlying molecular mechanism of melatonin's involvement in the HCPT‐induced side effects in cells, especially in the testicular cells, are poorly understood. In this study, we found that melatonin therapy significantly restored HCPT‐induced testicular cell damage and did not affect the antitumor effect of HCPT. Further analysis found that melatonin therapy suppressed HCPT‐induced DNA damage associated with ataxia‐telangiectasia mutated‐ and Rad3‐related and CHK1 phosphorylation levels in the testis. Changes in apoptosis‐associated protein levels (Bax, Bcl‐2, p53, and Cleaved caspase‐3) and in reactive oxygen species‐associated proteins (Nrf2 and Keap1) and index (malondialdehyde and glutathione) suggested that melatonin treatment relieved HCPT‐induced cell apoptosis and oxidative damage, respectively. Mechanistically, melatonin‐activated autophagy proteins (ATG7, Beclin1, and LC3bII/I) may induce p62‐dependent autophagy to degrade Keap1, eliciting Nrf2 from Keap1‐Nrf2 interaction to promote antioxidant enzyme expression such as HO‐1, which would salvage HCPT‐induced ROS production and mitochondrial dysfunction. Collectively, this study reveals that melatonin therapy may protect testicular cells from HCPT‐induced damage via the activation of autophagy, which alleviates oxidative stress, mitochondrial dysfunction, and cell apoptosis.
Treatment of Francisella philomiragia bacteremia in a dog
Authors: McAtee Rae , Wood Michael W. , Daniels Joshua B. , Lashnits Erin 
Published: May 2024

AbstractTo describe the diagnosis and successful treatment of systemic francisellosis in a dog. An 11‐year‐old female spayed Labrador retriever presented for progressive lethargy, hyporexia, and cough. The dog was febrile with a neutrophilia, nonregenerative anemia, thrombocytopenia, and had increased activity in serum of liver‐derived enzymes. Francisella philomiragia was isolated from aerobic blood culture. The dog was treated for 6 weeks with enrofloxacin orally. Repeated aerobic blood cultures after 2 and 6 weeks of antibiotic therapy were negative. The dog was clinically normal 7 months after diagnosis with no evidence of relapse.
Elemental and oxygen isotopic fractionation recorded in highly vaporized cosmic spherules from Widerøefjellet, Sør Rondane Mountains (East Antarctica)
Authors: Boonants Tom , Goderis Steven , Soens Bastien , Van Maldeghem Flore , Chernonozhkin Stepan M. , Vanhaecke Frank , van Ginneken Matthias , Snoeck Christophe , Claeys Philippe 
Published: May 2024

AbstractUpon passage through Earth's atmosphere, micrometeorites undergo variable degrees of melting and evaporation. Among the various textural and chemical groups recognized among cosmic spherules, that is, melted micrometeorites, a subset of particles may indicate anomalously high degrees of vaporization based on their chemical and isotopic properties. Here, a selection of such refractory element‐enriched cosmic spherules from Widerøefjellet (Sør Rondane Mountains, East Antarctica) is characterized for their petrographic features, major and trace element concentrations (N = 35), and oxygen isotopic compositions (N = 23). Following chemical classification, the highly vaporized particles can be assigned to either the “CAT‐like” or the “High Ca‐Al” cosmic spherule groups. However, through the combination of major and trace element concentrations and oxygen isotopic data, a larger diversity of processes and precursor materials are identified that lead to the final compositions of refractory element‐enriched particles. These include fragmentation, disproportional sampling of specific mineral constituents, differential melting, metal bead extraction, redox shifts, and evaporation. Based on specific element concentrations (e.g., Sc, Zr, Eu, Tm) and ratios (e.g., Fe/Mg, CaO + Al2O3/Sc + Y + Zr + Hf), and variations of O isotope compositions, “CAT‐like” and “High Ca‐Al” cosmic spherules likely represent a continuum between mineral endmembers from both primitive and differentiated parent bodies that experienced variable degrees of evaporation.
RUNX3 alleviates mitochondrial dysfunction and tubular damage by inhibiting TLR4/NF‐κB signalling pathway in diabetic kidney disease
Authors: Xiao Ling , Ye Gang 
Published: May 2024

AbstractAimThe impaired function of tubular mitochondria is critical in diabetic kidney disease (DKD) progression. RUNX3 is down‐regulated in DKD models. We intend to explore the effects of RUNX3 on mitochondrial dysfunction and renal tubule injury in DKD and related mechanisms.MethodsThe development of diabetes models involved injecting mice with streptozotocin while treating HK‐2 cells with high glucose (HG). By using immunohistochemical techniques, the renal localizations of RUNX3 were identified. Levels of adenosine triphosphate (ATP), mitochondrial membrane potential, and biochemical index were detected by appropriate kits. Reactive oxygen species (ROS) generation was assessed with dihydroethidium and MitoSOX Red staining. Apoptosis was assessed by flow cytometry and TUNEL. RUNX3 ubiquitination was measured.ResultsRUNX3 was mainly present in renal tubules. Overexpressing RUNX3 increased Mfn2, Mfn1, ATP levels, and mitochondrial membrane potential, reduced Drp1 and ROS levels and cell apoptosis, as well as Cyt‐C release into the cytoplasm. RUNX3 overexpression displayed a reduction in urinary albumin to creatinine ratio, Hemoglobin A1c, serum creatinine, and blood urea nitrogen. Overexpressing TLR4 attenuated the inhibitory effect of RUNX3 overexpression on mitochondrial dysfunction and cell apoptosis. HG promoted RUNX3 ubiquitination and SMURF2 expression. RUNX3 knockdown cancelled the inhibitory effect of SMURF2 on mitochondrial dysfunction and cell apoptosis.ConclusionSMURF2 interference inhibits RUNX3 ubiquitination and TLR4/NF‐κB signalling pathway, thereby alleviating renal tubule injury.image
F‐actin/DRP1 axis‐mediated mitochondrial fission promotes mitophagy in diabetic submandibular glands
Authors: Zhu Hou‐Wei , Wang Yi‐Ping , Zhang Qiu‐Fang , Wang Kai‐Di , Huang Yan , Xiang Ruo‐Lan 
Published: May 2024

AbstractBackgroundDiabetes is accompanied by a high prevalence of hyposalivation, causing severe damage to oral and systemic health. Mitochondrial dynamics play important roles in the pathogenesis of various diabetic complications; however, little is known about their roles in diabetic hyposalivation.Materials and MethodsA diabetic mouse model and a high glucose (HG)‐induced diabetic submandibular gland (SMG) cell model were employed.ResultsMore mitochondria surrounded by autophagosomes and higher expression of mitophagy‐related proteins were detected in the SMGs of diabetic mice and HG‐treated SMG cells. In diabetic SMGs, dynamin‐related protein 1 (DRP1) was upregulated, whereas mitofusin‐2 was downregulated both in vivo and in vitro. Shortened mitochondria and impaired mitochondrial functions were observed in the HG group. A DRP1‐specific inhibitor, mdivi‐1, suppressed mitochondrial fission and mitophagy, as well as restored mitochondrial functions in the HG condition. Moreover, the interaction of F‐actin and DRP1 was enhanced in the diabetic group. Inhibiting F‐actin with cytochalasin D repaired the injured effects of HG on mitochondrial dynamics and functions. Conversely, the F‐actin‐polymerization‐inducer jasplakinolide aggravated mitochondrial fission and dysfunction.ConclusionsF‐actin contributes to HG‐evoked mitochondrial fission by interacting with DRP1, which induces mitophagy and impairs mitochondrial function in SMG cells, ultimately damaging the SMG.