bluuebunny/crossref_metadata_2025_split huggingface dataset:
Bone quality, as measured by trabecular bone score in patients with adrenal incidentalomas with and without subclinical hypercortisolism
Authors: Eller-Vainicher Cristina , Morelli Valentina , Ulivieri Fabio Massimo , Palmieri Serena , Zhukouskaya Volha V , Cairoli Elisa , Pino Rosa , Naccarato Antonella , Scillitani Alfredo , Beck-Peccoz Paolo , Chiodini Iacopo 
Published: May 2012

Abstract
Patients with adrenal incidentalomas (AIs) and subclinical hypercortisolism (SH) have increased risk of fracture independent of bone mineral density (BMD) and possibly due to reduced bone quality. The trabecular bone score (TBS) has been proposed as a index of bone microarchitecture. The aim of the study was to investigate TBS in AI. In 102 AI patients, SH was diagnosed in the presence of at least two of the following: (1) urinary free cortisol &gt;70 µg/24 h (193.1 nmol/L); (2) cortisol after 1-mg dexamethasone suppression test (1-mg DST) &gt;3.0 µg/dL (82.8 nmol/L); or (3) adrenocorticotropic hormone (ACTH) &lt;10 pg/mL (&lt;2.2 pmol/L). In patients and in 70 matched controls, BMD was measured at lumbar spine (LS) and femur (neck [FN] and total [FT]) by dual X-ray absorptiometry and TBS was assessed in the region of LS-BMD; BMD and TBS data were reported as Z-scores. In patients, vertebral deformities were assessed by radiograph. Patients with SH (n = 34) had lower LS-BMD (−0.31 ± 1.17), FT-BMD (−0.29 ± 0.91), and TBS (−3.18 ± 1.21) than patients without SH (n = 68, 0.31 ± 1.42, p = 0.03; 0.19 ± 0.97, p = 0.01; −1.70 ± 1.54, p &lt; 0.0001, respectively) and controls (0.42 ± 1.52, p = 0.02; 0.14 ± 0.76, p = 0.02; −1.19 ± 0.99, p &lt; 0.0001, respectively). TBS was inversely correlated with 1-mg DST (β = −0.26, t = −2.79, p = 0.006) regardless of age, LS-BMD, body mass index (BMI), and gender. The presence of fracture was associated with low TBS alone (odds ratio [OR], 4.8; 95% confidence interval [CI], 1.85–12.42, p = 0.001) and with the cluster low TBS plus low LS-BMD (OR, 4.37; 95% CI, 1.71–11.4, p = 0.002), after adjustment for age, BMI, and gender. Low TBS plus low LS-BMD showed a good specificity (79%) for predicting fractures, whereas normal TBS (ie, &gt; −1.5) plus normal LS-BMD high specificity (88.1%) for excluding fractures. Finally, TBS predicted the occurrence of a new fracture in 40 patients followed for 24 months (OR, 11.2; 95%CI, 1.71–71.41, p = 0.012) regardless of LS-BMD, BMI, and age. In SH, bone quality, as measured by TBS, is altered. TBS is useful in detecting AI patients at risk of fractures. © 2012 American Society for Bone and Mineral Research.
Habitual flavonoid intakes are positively associated with bone mineral density in women
Authors: Welch Ailsa , MacGregor Alex , Jennings Amy , Fairweather-Tait Sue , Spector Tim , Cassidy Aedín 
Published: May 2012

Abstract
Dietary flavonoids exert bone-protective effects in animal models, but there is limited information on the effect of different flavonoid subclasses on bone health in humans. The aim of this observational study was to examine the association between habitual intake of flavonoid subclasses with bone mineral density (BMD) in a cohort of female twins. A total of 3160 women from the TwinsUK adult twin registry participated in the study. Habitual intakes of flavonoids and subclasses (flavanones, anthocyanins, flavan-3-ols, polymers, flavonols, and flavones) were calculated from semiquantitative food frequency questionnaires using an updated and extended U.S. Department of Agriculture (USDA) database. Bone density was measured using dual-energy X-ray absorptiometry. In multivariate analyses, total flavonoid intake was positively associated with higher BMD at the spine but not at the hip. For the subclasses, the magnitude of effect was greatest for anthocyanins, with a 0.034 g/cm2 (3.4%) and 0.029 g/cm2 (3.1%) higher BMD at the spine and hip, respectively, for women in the highest intake quintile compared to those in the lowest. Participants in the top quintile of flavone intake had a higher BMD at both sites; 0.021 g/cm2 (spine) and 0.026 g/cm2 (hip). At the spine, a greater intake of flavonols and polymers was associated with a higher BMD (0.021 and 0.024 g/cm2, respectively), whereas a higher flavanone intake was positively associated with hip BMD (0.008 g/cm2). In conclusion, total flavonoid intake was positively associated with BMD, with effects observed for anthocyanins and flavones at both the hip and spine, supporting a role for flavonoids present in plant-based foods on bone health. © 2012 American Society for Bone and Mineral Research.
N‐glycan biosynthesis inhibitors induce in vitro anticancer activity in colorectal cancer cells
Authors: de‐Freitas‐Junior Julio Cesar Madureira , Bastos Lilian Golçalves , Freire‐Neto Carlos Alberto , Rocher Bárbara Du , Abdelhay Eliana Saul Furquim Werneck , Morgado‐Díaz José Andrés 
Published: May 2012

AbstractDuring malignant transformation, changes in the expression profile of glycans may be involved in a variety of events, including the loss of cell–cell and cell–matrix adhesion, migration, invasion, and evasion of apoptosis. Therefore, modulation of glycan expression with drugs has promising therapeutic potential for various cancer types. In this study, we investigated the in vitro anticancer activity of the N‐glycan biosynthesis inhibitors (swainsonine and tunicamycin) in cells derived from colorectal cancer (CRC). We also examined whether these inhibitors are able to induce radiosensitization and toxicity when used in combination with cisplatin or irinotecan, two current anticancer drugs. Our results show that treatment with tunicamycin inhibits cellular mechanisms related to the malignant phenotype, such as anchorage‐dependent and anchorage‐independent colony formation, migration and invasion, in undifferentiated HCT‐116 colon cancer cells, whereas swainsonine only inhibits cell migration. We also observed that tunicamycin, but not swainsonine, caused radiosensitivity in HCT‐116 cells. Moreover, the combination of swainsonine with cisplatin or irinotecan enhanced their toxicity in HCT‐116 cells, while the combination of tunicamycin with these drugs had no effect. Given these results, we suggest that the modulation of N‐glycan biosynthesis appears to be a potential therapeutic tool for CRC treatment because inhibition of this process induced anticancer activity in vitro. Additionally, the inhibition of the N‐glycan biosynthesis in combination with chemotherapic drugs is a promising therapeutic strategy for enhancing radiation therapy. J. Cell. Biochem. 113: 2957–2966, 2012. © 2012 Wiley Periodicals, Inc.
MmNEU3 sialidase over‐expression in C2C12 myoblasts delays differentiation and induces hypertrophic myotube formation
Authors: Papini Nadia , Anastasia Luigi , Tringali Cristina , Dileo Loredana , Carubelli Ivan , Sampaolesi Maurilio , Monti Eugenio , Tettamanti Guido , Venerando Bruno 
Published: May 2012

AbstractSeveral factors affect the skeletal muscle differentiation process, in particular modifications of cell–cell contact, cell adhesion, and plasma membrane characteristics. In order to support the role of the plasma membrane‐associated sialidase NEU3 in skeletal muscle differentiation and to analyse which events of the process are mainly affected by this sialidase, we decided to stably over‐express MmNEU3 in C2C12 cells by a lentiviral vector and to investigate cell behavior during the differentiation process. Vitally stained C2C12 and NEU3 over‐expressing cells were counted to reveal modifications in differentiation induction. We found that NEU3 over‐expressing cells remained proliferative longer than control cells and delayed the onset of differentiation. Expression of p21, myogenic transcription factors, and myosin heavy chain (MHC), assessed by real time PCR, confirmed this behavior. In particular, no MHC‐positive myotubes were present in NEU3 over‐espressing cells as compared to wild type C2C12 cells at day 3 of differentiation. Moreover, NEU3 over‐expressing cells completed the differentiation process very quickly and formed hypertrophic myotubes. Analysis of MAPK/ERK pathway activation showed an increased ERK 1/2 phosphorylation in NEU3 over‐expressing cells at the beginning of differentiation. We postulate that sialidase NEU3, decreasing plasma membrane ganglioside GM3 content, affects the EGF receptor and the downstream signaling pathways, promoting proliferation and delaying differentiation. Furthermore NEU3 improves myoblast fusion probably via neural‐cell adhesion molecule (NCAM) desialylation. Therefore, this work further supports the central role of NEU3 as a key modulator in skeletal muscle differentiation, particularly in the myoblast fusion step. J. Cell. Biochem. 113: 2967–2978, 2012. © 2012 Wiley Periodicals, Inc.
Symmetrizer: Algorithmic determination of point groups in nearly symmetric molecules
Authors: Largent R. Jeffrey , Polik William F. , Schmidt J. R. 
Published: May 2012

AbstractSymmetry is an extremely useful and powerful tool in computational chemistry, both for predicting the properties of molecules and for simplifying calculations. Although methods for determining the point groups of perfectly symmetric molecules are well‐known, finding the closest point group for a “nearly” symmetric molecule is far less studied, although it presents many useful applications. For this reason, we introduce Symmetrizer, an algorithm designed to determine a molecule's symmetry elements and closest matching point groups based on a user‐adjustable tolerance, and then to symmetrize that molecule to a given point group geometry. In contrast to conventional methods, Symmetrizer takes a bottom‐up approach to symmetry detection by locating all possible symmetry elements and uses this set to deduce the most probable point groups. We explain this approach in detail, and assess the flexibility, robustness, and efficiency of the algorithm with respect to various input parameters on several test molecules. We also demonstrate an application of Symmetrizer by interfacing it with the WebMO web‐based interface to computational chemistry packages as a showcase of its ease of integration. © 2012 Wiley Periodicals, Inc.
Theoretical study of the excited‐state double proton transfer in the (3‐methyl‐7‐azaindole)‐(7‐azaindole) heterodimer
Authors: Yu Xue‐Fang , Yamazaki Shohei , Taketsugu Tetsuya 
Published: May 2012

AbstractExcited‐state double proton transfer (ESDPT) in the (3‐methyl‐7‐azaindole)‐(7‐azaindole) heterodimer is theoretically investigated by the long‐range corrected time‐dependent density functional theory method and the complete‐active‐space second‐order perturbation theory method. The calculated potential energy profiles exhibit a lower barrier for the concerted mechanism in the locally excited state than for the stepwise mechanism through the charge‐transfer state. This result suggests that the ESDPT in the isolated heterodimer is likely to follow the former mechanism, as has been exhibited for the ESDPT in the homodimer of 7‐azaindole. © 2012 Wiley Periodicals, Inc.
Ultrasound Assisted Emulsification Microextraction Based on dimetyl (E)‐2‐[(Z)‐1‐acetyl)‐2‐hydroxy‐1‐propenyl]‐2‐butenedioate for Determination of Total Amount of Iron in Water and Tea Samples
Authors: Khayatian Gholamreza , Hassanpoor Shahed 
Published: May 2012

AbstractAn ultrasound assisted emulsification microextraction (USAEME) is successfully used for extraction and determination of trace amount of iron in water and tea samples, followed by flame atomic absorption spectroscopy (FAAS). In this approach, a new synthetic ligand dimetyl (E)‐2‐[(Z)‐1‐acetyl)‐2‐hydroxy‐1‐propenyl]‐2‐butenedioate (DAHPB) is used as chelating agent and chloroform is selected as an extraction solvent. The factors influencing the complex formation and extraction by USAEME method are optimized. These factors are extraction solvent type as well as extraction volume, time, temperature, pH, and the amount of chelating agent. Under optimum conditions, an enrichment factor of 202.9 is obtained from only 7.1 mL of aqueous phase. The calibration graph using the preconcentration system for iron is linear between 40.0 and 800.0 μg L−1 with a detection limit of 7.4 μg L−1. The relative standard deviation (R.S.D) for ten replicate measurements of 500.0 μg L−1 of iron is 2.5%.
Investigation of Fatty Acid Composition of Ammi majus Seed Oil by Gas Chromatography Mass Spectrometry
Authors: Hussain Iqbal , Khan Shahid , Khan M. I. , Rehman Inayat Ur , Ahmed Majed 
Published: May 2012

AbstractThe Ammi majus seeds oil constituents of methyl ester derivatives of fatty acids were analyzed using Gas Chromatography coupled to mass spectrometer. The results obtained containing the saturated as well as unsaturated fatty acids of majus seeds oils. A total of 18 different components were identified and quantified. Methyl ester of linoleic acid was found in high concentration 9.00%, among the identified analytes of interest. In addition methyl ester of Oleic acid 5.60%, Palmitic acid 3.98% Linolenic acids 1.42% were found. Concentration of the rest of identified fatty acids analytes were less than 1%. Thus from the results it is apparent that due to the presence of high percentage of valuable analytes concentrations detected in the fatty acid of A. majus, has increased its importance for the consumption in the pharmaceuticals as well as its applications in the new formulations for various skin diseases to prevent and cure from different infections.
In vitro Inhibition Effect of Transition Metal Ions on Succinate‐Fumarate System in ATP Cycle
Authors: Farrukh Muhammad Akhyar , Majeed Sana 
Published: May 2012

AbstractOxidation of sodium succinate in aqueous solution by potassium ferricyanide was investigated. The effects of different reaction parameters such as initial concentration of succinate, concentration of ferricyanide, and influence of metal ions (Zn+2, Ni+2, Cu+2, Cd+2) on the oxidation of succinate were investigated at 25 ± 0.05 °C. Large rate enhancements were observed in the redox reaction between succinate and ferricyanide with increasing concentrations of both oxidant and reductant. The kinetics results indicated that the succinate oxidation was significantly inhibited in the presence of metal ions. Pseudo first order rate constants values were found decreased with increase in concentrations of metal ions which reflected that inhibition rate was directly influenced with the metal ions concentration.
Synthesis of Silver Nanowires and Other Metal Nanostructures in Dialkylimidazolium Ionic Liquid
Authors: Selvam Tamil Selvi , Chi Kai‐Ming 
Published: May 2012

AbstractRoom temperature ionic liquids have attracted much research attention for the preparation of nanoparticles owing to their nonvolatile nature, thermally stable properties and inflammability. In this contribution, we have made an effort to fabricate the late transition metal — Ru, Rh, Ir, Ni, Pd, Pt, Ag, and Au — nanostructures by thermal decomposition of the corresponding organometallic precursors in the presence of various surfactants in ionic liquid [Omim][PF6]. Silver nanowires can be specifically generated by heating the mixture of (hfac)Ag(PMe3) and 1‐hexadecylamine with the mole ratio of 1:10 in [Omim][PF6] at 140 °C for 3 h. Characterization of these metal nanostructures were carried out by transmission electron microscope (TEM), scanning electron microscope (SEM), energy dispersive spectroscopy (EDS), and X‐ray powder diffraction analysis.
Zinc Chloride Anhydrous as New and Effective Catalyst for Conversion of Ketones and Aldehydes to Corresponding Gem‐dihydroperoxides
Authors: Khosravi Kaveh , Kazemi Samira 
Published: May 2012

AbstractZinc chloride anhydrous has been used as an efficient and new catalyst for conversion of ketones and aldehydes to corresponding gem‐dihydroperoxides by aqueous hydrogen peroxide (30%) in room temperature with excellent yields and notable reaction times.
The Known Maximal Partial Ovoids of Size of
Authors: Coolsaet Kris , De Beule Jan , Siciliano Alessandro 
Published: May 2012

AbstractWe present a description of maximal partial ovoids of size of the parabolic quadric as sharply transitive subsets of and show their connection with spread sets. This representation leads to an elegant explicit description of all known examples. We also give an alternative representation of these examples that are related to root systems. © 2012 Wiley Periodicals, Inc. J. Combin. Designs 21: 89–100, 2013
Intersection Matrices Revisited
Authors: Ghareghani N. , Ghorbani E. , Mohammad‐Noori M. 
Published: May 2012

AbstractSeveral intersection matrices of s‐subsets versus k‐subsets of a v‐set are introduced in the literature. We study these matrices systematically through counting arguments and generating function techniques. A number of new or known identities appear as natural consequences of this viewpoint; especially, use of the derivative operator and some related operators reveals some connections between intersection matrices and the “combinatorics of creation‐annihilation.” As application, the eigenvalues of several intersection matrices including some generalizations of the adjacency matrices of the Johnson scheme are derived; two new bases for the Bose–Mesner algebra of the Johnson scheme are introduced and the associated intersection numbers are obtained as well. Finally, we determine the rank of some intersection matrices. © 2012 Wiley Periodicals, Inc. J. Combin. Designs 20: 383–397, 2012
Discrepancies between Turkish Mothers’ and Adolescents’ Reports of Adolescents’ Emotion Regulation Difficulties
Authors: Sarıtaş Dilek , Gençöz Tülin 
Published: May 2012

ObjectivesThe aim of the present study was to examine discrepancies between Turkish mothers’ and adolescents’ reports in terms of adolescents’ difficulties in emotion regulation. In addition, the mediating role of mothers’ own emotion regulation difficulties between mothers’ psychological symptoms and the discrepancy on reports of adolescents’ difficulties in emotion regulation was examined.MethodA total of 595 first‐year high school students (300 females and 295 males) whose ages ranged between 14 and 17 years participated in the study. In addition, 365 mothers (61.34%) completed the questionnaires. Mean age of mothers was 41.86 years (standard deviation = 5.02) ranging from 32 to 61 years.ResultsThe results revealed that adolescents tended to report higher levels of difficulties in emotion regulation for themselves as compared with mothers’ report, and girls reported more difficulties for their emotion regulation than boys. It was also found that mothers' own emotion regulation difficulties mediated the relation between mothers’ psychological symptoms and discrepancy on reports of adolescents’ emotion regulation difficulties.ConclusionsMothers may be less likely to endorse their children's emotion regulation difficulties since they cannot observe various aspects of their children's emotion regulation. On the other hand, the mothers’ perception of their children's emotion regulation could be affected from their own emotion regulation difficulties, which could be a result of their psychological problems.
Bovine sperm raft membrane associated Glioma Pathogenesis‐Related 1‐like protein 1 (GliPr1L1) is modified during the epididymal transit and is potentially involved in sperm binding to the zona pellucida
Authors: Caballero Julieta , Frenette Gilles , D'Amours Olivier , Belleannée Clémence , Lacroix‐Pepin Nicolas , Robert Claude , Sullivan Robert 
Published: May 2012

AbstractGlioma pathogenesis‐related 1‐like protein1 (GliPr1L1) was identified by liquid chromatography‐tandem mass spectrometry analyses of proteins associated to bovine sperm lipid raft membrane domains. This protein belongs to the CAP superfamily including cysteine‐rich secretory proteins, Antigen 5 and pathogenesis‐related 1 protein. PCR analysis revealed that GliPr1L1 is expressed in testis and, at a much lower level, all along the epididymis. Western blotting showed a similar distribution of GliPr1L1 in testicular and epididymal tissue extracts. In the epididymal lumen, GliPr1L1 was associated with the maturing spermatozoa and epididymosomes all along the excurrent duct but was undetectable in the soluble fraction of epididymal fluid. The protein was detectable as multiple isoforms with a higher MW form in the testis and proximal caput. Treatments with PNGase F revealed that N‐glycosylation was responsible of multiple bands detected on Western blots. These results suggest that the N‐glycosylation moiety of GliPr1L1 is processed during the transit in the caput. Western blots demonstrated that GliPr1L1 was associated with the sperm plasma membrane preparation. GliPr1L1 is glycosyl phosphatidyl inositol (GPI) anchored to caput and cauda spermatozoa as demonstrated by the ability of phosphatidylinositol specific phospholipase C to release GliPr1L1 from intact sperm cells. Lipid raft membrane domains were separated from caput and cauda epididymal spermatozoa. GliPr1L1 was immunodetectable in the low buoyant density fractions where lipid rafts are distributed. GliPr1L1 was localized on sperm equatorial segment and neck. In vitro fertilization performed in presence of anti‐GliPr1L1 showed that this protein is involved in sperm–zona pellucida interaction. J. Cell. Physiol. 227: 3876–3886, 2012. © 2012 Wiley Periodicals, Inc.
αMβ2‐integrin‐intercellular adhesion molecule‐1 interactions drive the flow‐dependent trafficking of Guillain–Barré syndrome patient derived mononuclear leukocytes at the blood–nerve barrier in vitro
Authors: Yosef Nejla , Ubogu Eroboghene E. 
Published: May 2012

AbstractThe mechanisms of hematogenous leukocyte trafficking at the human blood–nerve barrier (BNB) are largely unknown. Intercellular adhesion molecule‐1 (ICAM‐1) has been implicated in the pathogenesis of Guillain–Barré syndrome (GBS). We developed a cytokine‐activated human in vitro BNB model using primary endoneurial endothelial cells. Endothelial treatment with 10 U/ml tissue necrosis factor‐α and 20 U/ml interferon‐γ resulted in de novo expression of pro‐inflammatory chemokines CCL2, CXCL9, CXCL11, and CCL20, with increased expression of CXCL2–3, CXCL8, and CXCL10 relative to basal levels. Cytokine treatment induced/enhanced ICAM‐1, E‐ and P‐selectin, vascular cell adhesion molecule‐1 and the alternatively spliced pro‐adhesive fibronectin variant, fibronectin connecting segment‐1 expression in a time‐dependent manner, without alterations in junctional adhesion molecule‐A expression. Lymphocytes and monocytes from untreated GBS patients express ICAM‐1 counterligands, αM‐ and αL‐integrin, with differential regulation of αM‐integrin expression compared to healthy controls. Under flow conditions that mimic capillary hemodynamics in vivo, there was a >3‐fold increase in total GBS patient and healthy control mononuclear leukocyte adhesion/migration at the BNB following cytokine treatment relative to the untreated state. Function neutralizing monoclonal antibodies against human αM‐integrin (CD11b) and ICAM‐1 reduced untreated GBS patient mononuclear leukocyte trafficking at the BNB by 59% and 64.2%, respectively. Monoclonal antibodies against αL‐integrin (CD11a) and human intravenous immunoglobulin reduced total leukocyte adhesion/migration by 22.8% and 17.6%, respectively. This study demonstrates differential regulation of αM‐integrin on circulating mononuclear cells in GBS, as well as an important role for αM‐integrin–ICAM‐1 interactions in pathogenic GBS patient leukocyte trafficking at the human BNB in vitro. J. Cell. Physiol. 227: 3857–3875, 2012. © 2012 Wiley Periodicals, Inc.