bluuebunny/crossref_metadata_2025_split huggingface dataset:
Local tissue reaction after the application of topical hemostatic agents in a rat partial nephrectomy model
Authors: Chalupová Marta , Suchý Pavel , Pražanová Gabriela , Bartošová Ladislava , Sopuch Tomáš , Havelka Pavel 
Published: March 2012

AbstractVarious hemostatics are used for renal surgical procedures. We investigated the hemostatic efficacy of cellulose derivatives on the model of partial nephrectomy in rats focusing on the local reaction of renal parenchyma. A total of 50 Wistar rats were divided into five groups of 10 animals each. Partial nephrectomy of the caudal pole without hilar vascular control was performed. Oxidized cellulose (OC), sodium salt of oxycellulose (OCN), carboxymethyl cellulose (CMC), dialdehyde cellulose (DAC), and gelatin‐based hemostatic (C) were applied to the bleeding wounds. The time to hemostasis was monitored. Half of the animals were euthanized after 3 days, the second half 30 days from the experiment start date. The left kidney was excised and subjected to histopathological examination. The biochemical data was subjected to statistical analysis. The time to hemostasis in all groups was significantly less than in the C group (in OC p = 0.0057, OCN p = 0.0039, CMC and DAC p = 0.0001). In the C group, massive hemorrhages and necrosis did occur. In the OC and OCN groups, there were regenerative changes, a receding inflammatory reaction and hemorrhage. DAC caused an immune reaction and massive interstitial hemorrhages with biochemical signs of liver damage. Parenchyma in CMC revealed a reduction of necrosis and interstitial hemorrhages with regenerative processes. The most effective hemostatics were CMC and OC, achieving the best results both in the time to hemostasis, and for histopathological evaluation. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2012.
Human chorionic gonadotropin interactions with immobilized anti‐hCG studied by quartz‐crystal microbalance with dissipation monitoring
Authors: Chung Natalie N. , Hamlin Rebecca E. , Zwang Theodore J. , Johal Malkiat S. 
Published: March 2012

AbstractPrevious studies have found that commercial human pregnancy tests are often too insensitive to function to their advertised >99% accuracy. Improper orientation of proteins used for recognition of ligands in sensors can often prevent the binding site from being available to the ligand, thereby decreasing sensor sensitivity. We have developed a simple method for the immobilization of anti‐human chorionic gonadotropin on a sensor surface that maximizes its sensitivity by ensuring ligand binding sites are exposed and densely packed. This surface also has an improved regenerative capacity over previously reported human chorionic gonadotropin sensors, retaining 99% of initial sensitivity after six regeneration cycles with 8M urea. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2012.
Enhancement of bony in‐growth to metal implants by combining controlled hydroxyapatite coating and heat treatment
Authors: Oron Amir , Agar Gauriel , Oron Uri , Stein Anat 
Published: March 2012

AbstractThe rate of bony in‐growth to heat‐treated and controlled hydroxyapatite metal implants made of either titanium alloy (Ti‐6Al‐4V) or stainless steel (SS) 316L inserted to the medullar canal of the femur in rats was investigated. It was found that while partial coverage of hydroxyapatite (HA) did not cause a significant elevation of their bonding strength when compared with nonheated implants, HA, and heat treatment caused a significant (p < 0.01) elevation of 3.1‐fold in the bonding strength of the implants to the host bone. A similar phenomenon to that found for the titanium alloy implants was found to be true for the SS implants as well. It is concluded that the novel approach presented in this article, that is, to heat treat implants as well as controlled partial coating of them by HA, prior to their insertion to host bone, produce an enhancement of bone growth to metal implants greater than utilization of each method alone. Our findings may be used to further enhance bony in‐growth to metal implants in several clinical settings, producing avid implants with superior integration capabilities. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2012.
Improving cellular function and immune protection via layer‐by‐layer nanocoating of pancreatic islet β‐cell spheroids cocultured with mesenchymal stem cells
Authors: Bhaiji Tasneem , Zhi Zheng‐Liang , Pickup John C. 
Published: March 2012

AbstractIslet transplantation as a therapy for type 1 diabetes is currently limited by lack of primary transplant material from human donors and post‐transplantation loss of islets caused by adverse immune and nonimmune reactions. This study aimed to develop a novel strategy to create microenvironment for islets via integration of nanoencapsulation with cell cocultures, thereby enhancing their survival and function. The nanoencapsulation was achieved via layer‐by‐layer deposition of phosphorycholine‐modified poly‐L‐lysine/heparin leading to the formation of nanometer‐thick multilayer coating on islets. Spheroids formed by coculturing MIN6 β‐cells with mesenchymal stem cells in suspension were used as the tool for testing encapsulation. Coculturing MSCs with MIN6 cells allowed the cell constructs to enhance structural and morphologic stability with improved insulin secretory function and render them less susceptible to inflammatory cytokine‐induced apoptosis. Combining nanoencapsulation with coculture of MSCs/MIN6 resulted in higher glucose responsiveness, and lower antibody binding and apoptosis‐inducing effects of cytokines. This strategy of nanoencapsulating islet cocultures appears promising to improve cellular delivery of insulin for treating type 1 diabetes. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
In vitro assessment of Staphylococcus epidermidis and Staphylococcus aureus adhesion on TiO2 nanotubes on Ti–6Al–4V alloy
Authors: Pérez‐Jorge Concepción , Conde Ana , Arenas Maria A. , Pérez‐Tanoira Ramón , Matykina Endhze , de Damborenea Juan J. , Gómez‐Barrena Enrique , Esteban Jaime 
Published: March 2012

AbstractThe aim of this study was to evaluate Staphylococcus sp. adhesion to modified surfaces of titanium alloy (Ti–6Al–4V). Specimens of Ti–6Al–4V alloy 6‐4 ELI‐grade 23 that meets the requirements of ASTM F136 2002A (AMS 2631B class A1) were anodized in a mixture of sulfuric/hydrofluoric acid at 20 V for 5 and 60 min to form nanoporous (NP) and nanotubular (NT) oxide layers with pore diameter of 20 and 100 nm, respectively. The amount of fluorine incorporated in the oxide films from the electrolyte was 6 and 4 wt %, respectively. Bacterial adherence was studied using laboratory strains and six clinical strains each of Staphylococcus aureus and Staphylococcus epidermidis. Lower adherence of laboratory strains was demonstrated on fluoride nanostructured surfaces in comparison with the fluoride‐free surfaces. Significant differences between clinical strains and laboratory strains were also found (p < 0.0001, Kruskal–Wallis test) when NP and NT specimens were compared with chemically polished (CP) surfaces. The results of the tests using multiple clinical strains confirmed a decrease in bacterial adherence on F‐containing titanium oxide surfaces, suggesting a potential applicability of this surface, with a confirmed added value of decreasing clinical staphylococci adherence, for medical prosthetic devices. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
Electrospinning adipose tissue‐derived extracellular matrix for adipose stem cell culture
Authors: Francis Michael P. , Sachs Patrick C. , Madurantakam Parthasarathy A. , Sell Scott A. , Elmore Lynne W. , Bowlin Gary L. , Holt Shawn E. 
Published: March 2012

AbstractBasement membrane‐rich extracellular matrices, particularly murine sarcoma‐derived Matrigel, play important roles in regenerative medicine research, exhibiting marked cellular responsesin vitroandin vivo, although with limited clinical applications. We find that a human‐derived matrix from lipoaspirate fat, a tissue rich in basement membrane components, can be fabricated by electrospinning and used to support cell culture. We describe practical applications and purification of extracellular matrix (ECM) from adipose tissue (At‐ECM) and its use in electrospinning scaffolds and adipose stem cell (ASC) culture. The matrix composition of this purified and electrospun At‐ECM was assessed histochemically for basement membrane, connective tissue, collagen, elastic fibers/elastin, glycoprotein, and proteoglycans. Each histochemical stain was positive in fat tissue, purified At‐ECM, and electrospun At‐ECM, and to some extent positive in a 10:90 blend with polydioxanone (PDO). We also show that electrospun At‐ECM, alone and blended with PDO, supports ASC attachment and growth, suggesting that electrospun At‐ECM scaffolds support ASC cultivation. These studies show that At‐ECM can be isolated and electrospun as a basement membrane‐rich tissue engineering matrix capable of supporting stem cells, providing the groundwork for an array of future regenerative medicine advances. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
Biocompatible magnetite nanoparticles with varying silica‐coating layer for use in biomedicine: Physicochemical and magnetic properties, and cellular compatibility
Authors: Singh Rajendra K. , Kim Tae‐Hyun , Patel Kapil D. , Knowles Jonathan C. , Kim Hae‐Won 
Published: March 2012

AbstractMagnetic nanoparticles (MNPs) are considered highly useful in therapeutic and diagnostic applications. However, MNPs require surface modification to promote dispersibility in aqueous solutions and thus biocompatibility. In this article, the authors modified MNPs with inorganic silica layer to create silica‐coated magnetite nanoparticles (MNP@Si) via sol–gel process. Synthesis involves hydrolysis and condensation steps using tetraethylorthosilicate (TEOS) in methanol/ polyethylene glycol (PEG) solution and ammonia catalyst. Nanoparticles were characterized in terms of morphology, particle size, crystalline phase, chemical‐bond structure, surface charge and magnetic properties: in particular, the MNP@Si size was easily tunable through alteration of the Fe3O4‐to‐TEOS ratio. As this ratio increased, the MNP@Si size decreased from 270 to 15 nm whilst maintaining core 12‐nm MNP particle size, indicating decrease in thickness of the silica coating. All MNP@Si, in direct contrast to uncoated MNPs, showed excellent stability in aqueous solution. The particles' physicochemical and magnetic properties systematically varied with size (coating thickness), and the zeta potential diminished toward negative values, while magnetization increased as the coating thickness decreased. 15‐nm MNP@Si showed excellent magnetization (about 64.1 emu/g), almost comparable to that of uncoated MNPs (70.8 emu/g). Preliminary in vitro assays confirmed that the silica layer significantly reduced cellular toxicity as assessed by increase in cell viability and reduction in reactive oxygen species production during 48 h of culture. Newly‐developed MNP@Si, with a high capacity for magnetization, water‐dispersibility, and diminished cell toxicity, may be potentially useful in diverse biomedical applications, including delivery of therapeutic and diagnostic biomolecules. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
Porous polycaprolactone/nanohydroxyapatite tissue engineering scaffolds fabricated by combining NaCl and PEG as co‐porogens: Structure, property, and chondrocyte–scaffold interaction in vitro
Authors: Liu Li , Wang Yuanyuan , Guo Shengrong , Wang Zhenyu , Wang Wei 
Published: March 2012

AbstractIn this study, porous polycaprolactone/nanohydroxyapatite (PCL/nHA) composite scaffolds were fabricated using a modified melt‐molding/leaching technique, by the combination of salt particulate (NaCl) and water‐soluble polymer (PEG) as co‐porogens. The porogens were kept at a constant proportion of 70% in the blends but varied in the NaCl/PEG ratio and the PEG variety to generate PCL/nHA scaffolds with various pore architectures. The resultant composite scaffolds were investigated on their morphologies, physicochemical properties, mechanical properties, and in vitro degradation. The cell–scaffold interactions were evaluated in vitro using chondrocyte. Generally, the PCL/nHA scaffolds exhibited multimodal pore morphologies consisting of macropores and interconnected micropores, created by the extraction of NaCl particulate and continuous PEG phase. The evolution of porogens led to much effect on the overall pore architecture of the scaffolds; subsequently, their physiochemical and mechanical properties and degradation behaviors, as well as the cell binding and proliferation. The PCL/nHA scaffold prepared from NaCl/PEG 4000 (20/50) presented more macropores (>50 μm) with interconnectivity and showed higher strength and improved bioactivity than the others. All of these results suggest promising potentials of PCL/nHA scaffolds developed in this study desired for cartilage tissue engineering. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 2012.
MicroRNAs miR‐96, miR‐124, and miR‐199a regulate gene expression in human bone marrow‐derived mesenchymal stem cells
Authors: Laine Salla K. , Alm Jessica J. , Virtanen Sanna P. , Aro Hannu T. , Laitala‐Leinonen Tiina K. 
Published: March 2012

AbstractMicroRNAs are small non‐coding RNAs that control gene expression at the post‐transcriptional level by binding to 3′‐untranslated regions (3′‐UTR) of their target mRNAs. They present a promising tool to delineate the molecular mechanisms regulating differentiation of human mesenchymal stromal cells (hMSCs) and to improve the controlled differentiation of hMSCs in therapeutic applications. Here we show that three microRNAs, miR‐96, miR‐124, and miR‐199a, were differentially expressed during osteogenic, adipogenic, and chondrogenic induction of human bone marrow‐derived MSCs. miR‐96 expression was increased during osteogenesis and adipogenesis, but not during chondrogenesis. miR‐124 was exclusively expressed in adipocytes, whereas miR‐199a was upregulated in osteoblasts and chondrocytes. Furthermore, functional studies with synthetic miRNA precursors and inhibitors demonstrated that miR‐96, miR‐124, and miR‐199a regulated the expression of genes important for hMSC differentiation, such as aggrecan, transcription factor SOX9, and fatty acid binding protein 4 (FABP4). Modulation of miR‐96, miR‐124, and miR‐199a expression may thus be useful in specific targeting of hMSC differentiation, for e.g., MSC‐based therapies. J. Cell. Biochem. 113: 2687–2695, 2012. © 2012 Wiley Periodicals, Inc.
Overexpression of forkhead box J2 can decrease the migration of breast cancer cells
Authors: Wang Yingying , Yang Shuyun , Ni Qichao , He Song , Zhao Yunhong , Yuan Qin , Li Chunmiao , Chen Hongwei , Zhang Li , Zou Lin , Shen Aiguo , Cheng Chun 
Published: March 2012

AbstractThe prognosis of breast cancer patients with metastases is generally poor, so it is essential to elucidate related molecules mechanisms. Forkhead Box J2 (FOXJ2) is a member of Forkhead Box transcription factors, many of which have been reported to participate in tumor migration and invasion. In this study, we showed the expression of FOXJ2 was higher in primary breast cancer tissues without lymph nodes metastases than those with, and there was statistical significance between the expression of FXOJ2 and the clinical factors. Hence, we identified a novel function of metastasis, which was not previously known for FOXJ2. Overexpression of FOXJ2 decreased the motility property of highly migrative MDA‐MB‐231 cells in vitro by wound healing assays and trans‐well migration assays, and it was concurrent with the increased expression of epithelial marker E‐cadherin and the decreased expression of mesenchymal marker vimentin by Western blot analysis, reverse transcription PCR analysis, and immunofluorescence analysis. Consistent with these observations, the repression of FOXJ2 in weakly metastatic MCF‐7 cells remarkably promoted cellular motility. Our study demonstrates that FOXJ2 can inhibit the metastasis of human breast cancer by regulating the EMT key markers E‐cadherin and vimentin. J. Cell. Biochem. 113: 2729–2737, 2012. © 2012 Wiley Periodicals, Inc.
PDE5 inhibitor promotes melanin synthesis through the PKG pathway in B16 melanoma cells
Authors: Zhang Xiaodong , Yan Guirui , Ji Jun , Wu Jingwei , Sun Xiaoyun , Shen Jingshan , Jiang Hualiang , Wang Heyao 
Published: March 2012

AbstractPDE inhibitors could increase cellular cGMP levels and are used to treat erectile dysfunction as well as pulmonary arterial hypertension. cGMP production was reported to be necessary for UVB‐induced melanin synthesis, however, the effect of PDE5 inhibitor on melanin synthesis has not been examined. We found that PDE5 inhibitor (sildenafil or vardenafil) and the cGMP analog 8‐CPT‐cGMP stimulated CREB phosphorylation, leading to increased tyrosinase expression and melanin synthesis, which was counteracted by KT5823, a selective cGMP‐dependent protein kinase (PKG) inhibitor. However, KT5823 did not affect cAMP‐elevating agent‐mediated melanin synthesis, indicating that KT5823 selectively inhibited cGMP‐induced melanin synthesis. This is the first study to find that PDE5 inhibitor can promote melanin synthesis and reveal that PKG‐dependent CREB phosphorylation and tyrosinase expression is involved in cGMP‐induced melanin synthesis. Our results suggest that PDE5 inhibitor may be beneficial for the treatment of hypopigmentation diseases. J. Cell. Biochem. 113: 2738–2743, 2012. © 2012 Wiley Periodicals, Inc.
Acetylcholine induces mesenchymal stem cell migration via Ca2+/PKC/ERK1/2 signal pathway
Authors: Tang Jun‐Ming , Yuan Jie , Li Qing , Wang Jia‐Ning , Kong Xia , Zheng Fei , Zhang Lei , Chen Long , Guo Lin‐Yun , Huang Yong‐Hang , Yang Jian‐Ye , Chen Shi‐You 
Published: March 2012

AbstractAcetylcholine (ACh) plays an important role in neural and non‐neural function, but its role in mesenchymal stem cell (MSC) migration remains to be determined. In the present study, we have found that ACh induces MSC migration via muscarinic acetylcholine receptors (mAChRs). Among several mAChRs, MSCs express mAChR subtype 1 (m1AChR). ACh induces MSC migration via interaction with mAChR1. MEK1/2 inhibitor PD98059 blocks ERK1/2 phosphorylation while partially inhibiting the ACh‐induced MSC migration. InsP3Rs inhibitor 2‐APB that inhibits MAPK/ERK phosphorylation completely blocks ACh‐mediated MSC migration. Interestingly, intracellular Ca2+ ATPase‐specific inhibitor thapsigargin also completely blocks ACh‐induced MSC migration through the depletion of intracellular Ca2+ storage. PKCα or PKCβ inhibitor or their siRNAs only partially inhibit ACh‐induced MSC migration, but PKC‐ζ siRNA completely inhibits ACh‐induced MSC migration via blocking ERK1/2 phosphorylation. These results indicate that ACh induces MSC migration via Ca2+, PKC, and ERK1/2 signal pathways. J. Cell. Biochem. 113: 2704–2713, 2012. © 2012 Wiley Periodicals, Inc.
ATF5, a possible regulator of osteogenic differentiation in human adipose‐derived stem cells
Authors: Leong David Tai , Abraham Mohan Chothirakottu , Gupta Anurag , Lim Thiam‐Chye , Chew Fook Tim , Hutmacher Dietmar Werner 
Published: March 2012

AbstractThe regulatory pathways involved in maintaining the pluripotency of embryonic stem cells are partially known, whereas the regulatory pathways governing adult stem cells and their “stem‐ness” are characterized to an even lesser extent. We, therefore, screened the transcriptome profiles of 20 osteogenically induced adult human adipose‐derived stem cell (ADSC) populations and investigated for putative transcription factors that could regulate the osteogenic differentiation of these ADSC. We studied a subgroup of donors' samples that had a disparate osteogenic response transcriptome from that of induced human fetal osteoblasts and the rest of the induced human ADSC samples. From our statistical analysis, we found activating transcription factor 5 (ATF5) to be significantly and consistently down‐regulated in a randomized time‐course study of osteogenically differentiated adipose‐derived stem cells from human donor samples. Knockdown of ATF5 with siRNA showed an increased sensitivity to osteogenic induction. This evidence suggests a role for ATF5 in the regulation of osteogenic differentiation in adipose‐derived stem cells. To our knowledge, this is the first report that indicates a novel role of transcription factors in regulating osteogenic differentiation in adult or tissue specific stem cells. J. Cell. Biochem. 113: 2744–2753, 2012. © 2012 Wiley Periodicals, Inc.
Regulation of gene expression in retrovirus vectors by X‐ray and proton beam radiation with artificially constructed promoters
Authors: Ogawa Ryohei , Morii Akihiro , Watanabe Akihiko , Cui Zheng‐Guo , Kagiya Go , Fukuda Shigekazu , Kume Kyo , Hasegawa Takashi , Hatashita Masanori , Izumi Hironori , Ishimoto Tetsuya , Feril Loreto B. 
Published: March 2012

AbstractBackgroundWe previously obtained an X‐ray responsive promoter from 11 promoters that we constructed. In the present study, we aimed to determine the efficiency of our promoter construction method. In addition, the reactivity of the promoter to X‐rays in vivo is also investigated.MethodsPromoters constructed by linking the TATA box to randomly combined binding sequences of transcription factors activated by radiation were cloned to prepare a promoter library. Combinations of promoters and various genes were stably‐transfected into HeLa cells to establish recombinant cell lines, which were then exposed to X‐rays or a proton beam to observe gene expression enhancement with or without anti‐oxidants. Tumors of luciferase‐expressing recombinant cells on mice were exposed to X‐rays and promoter activation was evaluated by detecting bioluminescence. As a model for in vitro suicide gene therapy, fcy::fur‐expressing recombinant cells were exposed to X‐rays before incubation with 5‐fluorocytosin. Cell viability was determined with WST‐8.ResultsTwenty‐five of the 62 promoters in the library enhanced luciferase activity over five‐fold, 6 h after receiving 10 Gy of X‐ray irradiation, suggesting the effectiveness of our method. Luciferase activity in recombinant cells was enhanced by X‐rays and, to a lesser extent, by a proton beam. Anti‐oxidants attenuated the enhancement, suggesting the involvement of oxidative stress. Promoters were less reactive to X‐rays in tumors on mice. In our suicide gene therapy model, survival of post‐irradiated cells decreased dose‐dependently with 5‐fluorocytosin.ConclusionsOur method was efficient in generating radiation responsive promoters. Furthermore, we have successfully shown a potential therapeutic use for one of these promoters. Copyright © 2012 John Wiley & Sons, Ltd.