bluuebunny/crossref_metadata_2025_split huggingface dataset:
Right Kocher’s incision: a feasible and effective incision for right hemicolectomy: a retrospective study
Authors: Theodosopoulos Theodosios , Yiallourou Anneza I , Dafnios Nicolaos , Polymeneas George , Papaconstantinou Ioannis , Staikou Chrysoula , Vassiliou Ioannis , Smyrniotis Vassilis , Fotopoulos Alexios 
Published: June 2012

Abstract

Background
The choice of surgical incision is determined by access to the surgical field, particularly when an oncological resection is required. Special consideration is also given to other factors, such as postoperative pain and its sequelae, fewer complications in the early postoperative period and a lower occurrence of incisional hernias. The purpose of this study is to compare the right Kocher’s and the midline incision, for patients undergoing right hemicolectomy, by focusing on short- and longterm results.

Methods
Between 1995 and 2009, hospital records for 213 patients that had undergone a right hemicolectomy for a right- sided adenocarcinoma were retrospectively studied. 113 patients had been operated via a Kocher’s and 100 via a midline incision. Demographic details, operative data (explorative access to the peritoneal cavity, time of operation), recovery parameters (time with IV analgesic medication, time to first oral fluid intake, time to first solid meal, time to discharge), and oncological parameters (lymph node harvest, TNM stage and resection margins) were analyzed. Postoperative complications were also recorded. The two groups were retrospectively well matched with respect to demographic parameters and oncological status of the tumor.

Results
The median length of the midline incision was slightly longer (12 vs. 10 cm, p < 0.05). The duration of the surgery for the Kocher’s incision group was significantly shorter (median time 70 vs 85 min, p < 0.001). In three patients we performed wedge resection of liver metastasis and in one patient we performed a typical right hepatectomy that lasted 190 min. No major operative complications were noted. There was no immediate or 30- day postoperative mortality. The Kocher’s incision group had a significantly shorter hospital stay (median time 5 vs 8 days). All patients underwent wide tumor excision and clear resection margins were obtained in all cases. No significant difference was noted regarding analgesia requirements and early postoperative complications. Late postoperative complications included 2 incisional hernias and three patients presented with one episode of obstructive ileus, that resolved conservatively.

Conclusions
The Kocher’s incision approach for right- sided colon cancer is technically feasible, safe and overall very well tolerated. It can achieve the same standards of tumor resection and surgical field accessibility as the midline approach, while reducing postoperative recovery.
Preparation and characterization of silk fibroin as a biomaterial with potential for drug delivery
Authors: Zhang Hao , Li Ling-ling , Dai Fang-yin , Zhang Hao-hao , Ni Bing , Zhou Wei , Yang Xia , Wu Yu-zhang 
Published: June 2012

Abstract

Background
Degummed silk fibroin from Bombyx mori (silkworm) has potential carrier capabilities for drug delivery in humans; however, the processing methods have yet to be comparatively analyzed to determine the differential effects on the silk protein properties, including crystalline structure and activity.

Methods
In this study, we treated degummed silk with four kinds of calcium-alcohol solutions, and performed secondary structure measurements and enzyme activity test to distinguish the differences between the regenerated fibroins and degummed silk fibroin.

Results
Gel electrophoresis analysis revealed that Ca(NO3)2-methanol, Ca(NO3)2-ethanol, or CaCl2-methanol treatments produced more lower molecular weights of silk fibroin than CaCl2-ethanol. X-ray diffraction and Fourier-transform infrared spectroscopy showed that CaCl2-ethanol produced a crystalline structure with more silk I (α-form, type II β-turn), while the other treatments produced more silk II (β-form, anti-parallel β-pleated sheet). Solid-State 13C cross polarization and magic angle spinning-nuclear magnetic resonance measurements suggested that regenerated fibroins from CaCl2-ethanol were nearly identical to degummed silk fibroin, while the other treatments produced fibroins with significantly different chemical shifts. Finally, enzyme activity test indicated that silk fibroins from CaCl2-ethanol had higher activity when linked to a known chemotherapeutic drug, L-asparaginase, than the fibroins from other treatments.

Conclusions
Collectively, these results suggest that the CaCl2-ethanol processing method produces silk fibroin with biomaterial properties that are appropriate for drug delivery.
Pyrene-POSS nanohybrid as a dispersant for carbon nanotubes in solvents of various polarities: its synthesis and application in the preparation of a composite membrane
Authors: Majeed Shahid , Filiz Volkan , Shishatskiy Sergey , Wind Jan , Abetz Clarissa , Abetz Volker 
Published: June 2012

Abstract
In this study we report the preparation of nanohybrid dispersant molecules based on pyrene and polyhedral oligomeric silsesquioxanes for non-covalent functionalization of multi-walled carbon nanotubes (MWCNTs). The prepared dispersant improves the dispersion of MWCNTs in organic solvents with very different polarities such as tetrahydrofuran, toluene, and n-hexane. The functionalized MWCNTs were used to introduce conductivity into polydimethylsiloxane membranes which can be used for electrostatic discharge applications.
A reduced-complexity scheme using message passing for location tracking
Authors: Chiou Yih-Shyh , Tsai Fuan , Wang Chin-Liang , Huang Chin-Tseng 
Published: June 2012

Abstract
This article presents a low-complexity and high-accuracy algorithm using message-passing approach to reduce the computational load of the traditional tracking algorithm for location estimation. In the proposed tracking scheme, a state space model for the location-estimation problem can be divided into many mutual-interaction local constraints based on the inherent message-passing features of factor graphs. During each iteration cycle, the message with reliability information is passed efficiently with an adaptive weighted technique and the error propagation law, and then the message-passing approach based on prediction-correction recursion is to simplify the implementation of the Bayesian filtering approach for location-estimation and tracking systems. As compared with a traditional tracking scheme based on Kalman filtering (KF) algorithms derived from Bayesian dynamic model, the analytic result and the numerical simulations show that the proposed forward and one-step backward tracking approach not only can achieve an accurate location very close to the traditional KF tracking scheme, but also has a lower computational complexity.
Heterogeneous virulence of pandemic 2009 influenza H1N1 virus in mice
Authors: Farooqui Amber , Leon Alberto J , Lei Yanchang , Wang Pusheng , Huang Jianyun , Tenorio Raquel , Dong Wei , Rubino Salvatore , Lin Jie , Li Guishuang , Zhao Zhen , Kelvin David J 
Published: June 2012

Abstract

Background
Understanding the pathogenesis of influenza infection is a key factor leading to the prevention and control of future outbreaks. Pandemic 2009 Influenza H1N1 infection, although frequently mild, led to a severe and fatal form of disease in certain cases that make its virulence nature debatable. Much effort has been made toward explaining the determinants of disease severity; however, no absolute reason has been established.

Results
This study presents the heterogeneous virulence of clinically similar strains of pandemic 2009 influenza virus in human alveolar adenocarcinoma cells and mice. The viruses were obtained from patients who were admitted in a local hospital in China with a similar course of infection and recovered. The A/Nanchang/8002/2009 and A/Nanchang/8011/2009 viruses showed efficient replication and high lethality in mice while infection with A/Nanchang/8008/2009 was not lethal with impaired viral replication, minimal pathology and modest proinflammatory activity in lungs. Sequence analysis displayed prominent differences between polymerase subunits (PB2 and PA) of viral genomes that might correlate with their different phenotypic behavior.

Conclusions
The study confirms that biological heterogeneity, linked with the extent of viral replication, exists among pandemic H1N1 strains that may serve as a benchmark for future investigations on influenza pathogenesis.
Assessment of adherence to the CONSORT statement for quality of reports on randomized controlled trial abstracts from four high-impact general medical journals
Authors: Ghimire Saurav , Kyung Eunjung , Kang Wonku , Kim Eunyoung 
Published: June 2012

AbstractBackgroundThe extended Consolidated Standards of Reporting Trials (CONSORT) Statement for Abstracts was developed to improve the quality of reports of randomized controlled trials (RCTs) because readers often base their assessment of a trial solely on the abstract. To date, few data exist regarding whether it has achieved this goal. We evaluated the extent of adherence to the CONSORT for Abstract statement for quality of reports on RCT abstracts by four high-impact general medical journals.MethodsA descriptive analysis of published RCT abstracts in The New England Journal of Medicine (NEJM), The Lancet, The Journal of American Medical Association (JAMA), and the British Medical Journal (BMJ) in the year 2010 was conducted by two reviewers, independently extracting data from a MEDLINE/PubMed search.ResultsWe identified 271 potential RCT abstracts meeting our inclusion criteria. More than half of the abstracts identified the study as randomized in the title (58.7%; 159/271), reported the specific objective/hypothesis (72.7%; 197/271), described participant eligibility criteria with settings for data collection (60.9%; 165/271), detailed the interventions for both groups (90.8%; 246/271), and clearly defined the primary outcome (94.8%; 257/271). However, the methodological quality domains were inadequately reported: allocation concealment (11.8%; 32/271) and details of blinding (21.0%; 57/271). Reporting the primary outcome results for each group was done in 84.1% (228/271). Almost all of the abstracts reported trial registration (99.3%; 269/271), whereas reports of funding and of harm or side effects from the interventions were found in only 47.6% (129/271) and 42.8% (116/271) of the abstracts, respectively.ConclusionsThese findings show inconsistencies and non-adherence to the CONSORT for abstract guidelines, especially in the methodological quality domains. Improvements in the quality of RCT reports can be expected by adhering to existing standards and guidelines as expressed by the CONSORT group.
Ileocolic intussusception due to a cecal endometriosis: Case report and review of literature
Authors: Emmanuel Rivkine , Léa Marciano , Claude Polliand , Antonio Valenti , Marianne Ziol , Christophe Poncelet , Christophe Barrat 
Published: June 2012

Abstract

Abstract
Cecal endometriosis and ileocolic intussusception due to a cecal endometriosis is extremely rare. We report a case of a woman who presented an ileocecal intussusception due to a cecal endometriosis. The patient gave two months history of chronic periombilical pain requiring regular hospital admission and analgesia. The symptoms were not related to menses. A laparotomy was performed and revealed an ileocolic intussusception. The abdominal exploration did not find any endometriosis lesion. Ileocaecal resection was performed. Microscopic examination showed a cystic component, lined by a regular cylindric epithelium. Foci of endometrial tissu were oberved in the cecal subserosa and muscularis mucosal, with irregular endometrial glands lined by cylindric epithelium without atypia immunostained with CK7, and characteristic endometrial stroma immunostained with CD10. Cecal endometriosis and ileocolic intussusception due to a cecal endometriosis is extremely rare. Diagnose of etiology remains challenging due to the absence of clinical and radiological specific characteristics.

Virtual slide
The virtual slide(s) for this article can be found here:http://www.diagnosticpathology.diagnomx.eu/vs/2975867306869166
A cross-sectional single-centre study on the spectrum of Pompe disease, German patients: molecular analysis of the GAA gene, manifestation and genotype-phenotype correlations
Authors: Herzog Andreas , Hartung Ralf , Reuser Arnold J J , Hermanns Pia , Runz Heiko , Karabul Nesrin , Gökce Seyfullah , Pohlenz Joachim , Kampmann Christoph , Lampe Christina , Beck Michael , Mengel Eugen 
Published: June 2012

Abstract

Background
Pompe disease (Glycogen storage disease type II, GSD II, acid alpha-glucosidase deficiency, acid maltase deficiency, OMIM # 232300) is an autosomal-recessive lysosomal storage disorder due to a deficiency of acid alpha-glucosidase (GAA, acid maltase, EC 3.2.1.20, Swiss-Prot P10253). Clinical manifestations are dominated by progressive weakness of skeletal muscle throughout the clinical spectrum. In addition, the classic infantile form is characterised by hypertrophic cardiomyopathy.

Methods
In a cross-sectional single-centre study we clinically assessed 3 patients with classic infantile Pompe disease and 39 patients with non-classic presentations, measured their acid alpha-glucosidase activities and analysed their GAA genes.

Results
Classic infantile patients had nearly absent residual enzyme activities and a typical clinical course with hypertrophic cardiomyopathy until the beginning of therapy. The disease manifestations in non-classic patients were heterogeneous. There was a broad variability in the decline of locomotive and respiratory function. The age of onset ranged from birth to late adulthood and correlated with enzyme activities. Molecular analysis revealed as many as 33 different mutations, 14 of which are novel. All classic infantile patients had two severe mutations. The most common mutation in the non-classic group was c.-32-13 T > G. It was associated with a milder course in this subgroup.

Conclusions
Disease manifestation strongly correlates with the nature of the GAA mutations, while the variable progression in non-classic Pompe disease is likely to be explained by yet unknown modifying factors. This study provides the first comprehensive dataset on the clinical course and the mutational spectrum of Pompe disease in Germany.
Gastrointestinal stromal tumor of the rectum with scapular metastasis: a case report
Authors: Selcukbiricik Fatih , Tural Deniz , Ozturk Mehmet Akif , Dervisoglu Sergulen , Sager Sait , Hız Murat , Mandel Nil Molinas 
Published: June 2012

Abstract

Introduction
Gastrointestinal stromal tumors are rare tumors. They commonly metastasize within the abdominal cavity, particularly to the liver. Less commonly, metastases can be found in the bone.

Case presentation
We here present a case of metastasis to the scapula in a 54-year-old Caucasian male patient with an advanced gastrointestinal stromal tumor, which was subsequently successfully treated with resection and sunitinib.

Conclusion
The present study is, to the best of our knowledge, the second to describe scapular metastasis of a gastrointestinal stromal tumor. Our patient was treated by scapulectomy. The overwhelming majority of scapular tumors are metastases that arise from soft tissue, hepatocellular and thyroid tumors. Gastrointestinal stromal tumor metastasis occurs rarely. Scapular surgery can successfully provide local control of the disease. After the surgery, patients should continue with medical treatment.
Reasons physicians do not recommend and patients refuse adjuvant chemotherapy for stage III colon cancer: a population based chart review
Authors: El Shayeb Mohamed , Scarfe Andrew , Yasui Yutaka , Winget Marcy 
Published: June 2012

Abstract

Background
Surgery followed by adjuvant chemotherapy has been the standard of care for the treatment of stage III colon cancer since the early 1990’s. Despite this, large proportions of patients do not receive adjuvant chemotherapy. We aimed to identify physicians’ and patients’ reasons for treatment decisions.

Methods
A retrospective population based study was conducted that included all surgically treated stage III colon cancer patients diagnosed in Alberta between 2002 and 2005 who had an oncologist-consult to discuss post-surgical treatment options. Patient demographics and stage were obtained from the Alberta Cancer Registry. Chart reviews were conducted to extract treatment details, the oncologists’ reasons for not recommending chemotherapy, and patients’ reasons for refusing chemotherapy. The number and proportion of patients who were not recommended or refused chemotherapy were calculated.

Results
A total of 613 patients had surgery followed by an oncologist-consult. Overall, 168 (27%) patients did not receive chemotherapy. It was not recommended for 111 (18%) patients; the most frequent reason was presence of one or more co-morbidities (34%) or combination of co-morbidity and age or frailty (22%). Fifty-eight (9%) patients declined chemotherapy, 22% of whom declined due to concerns about toxicity.

Conclusion
Some co-morbidities are clinical indications for not receiving adjuvant chemotherapy, however, the high percentage of patients who were not recommended adjuvant chemotherapy due to co-morbidities according to clinical notes but who had a low Charlson co-morbidity score suggests variation in practice patterns of consulting oncologists. In addition, patients’ reasons for refusing treatment need to be systematically assessed to ensure patients’ preferences and treatment benefits are properly weighed when making treatment decisions.
First report of Toxoplasma gondii infection in market-sold adult chickens, ducks and pigeons in northwest China
Authors: Cong Wei , Huang Si-Yang , Zhou Dong-Hui , Xu Min-Jun , Wu Song-Ming , Yan Chao , Zhao Quan , Song Hui-Qun , Zhu Xing-Quan 
Published: June 2012

AbstractBackgroundToxoplasma gondiiinfection is a global concern, affecting a wide range of warm-blooded animals and humans worldwide, including poultry. Domestic and companion birds are considered to play an important role in the transmission ofT. gondiito humans and other animals. However, little information onT. gondiiinfection in domestic birds in Lanzhou, northwest China was available. Therefore, this study was performed to determine the seroprevalence ofT. gondiiinfection in domestic birds in Lanzhou, northwest China.MethodsIn the present study, the seroprevalence ofT. gondiiantibodies in 413 (305 caged and 108 free-range) adult chickens, 334 (111 caged and 223 free-range) adult ducks and 312 adult pigeons in Lanzhou, northwest China, were examined using the modified agglutination test (MAT).Results30 (7.26%) chickens, 38 (11.38%) ducks and 37 (11.86%) pigeons were found to be positive forT. gondiiantibodies at the cut-off of 1:5. The prevalences in caged and free-range chickens were 6.23% and 10.19% respectively, however, statistical analysis showed that the difference was not significant (P > 0.05). The seroprevalences in caged and free-range ducks were 6.31% and 13.90% respectively, but the difference was not statistically significant (P > 0.05).ConclusionsThe results of the present survey indicated the presence ofT. gondiiinfection in adult chickens, ducks and pigeons sold for meat in poultry markets in Lanzhou, northwest China, which poses a potential risk forT. gondiiinfection in humans and other animals in this region. This is the first seroprevalence study ofT. gondiiinfection in domestic birds in this region.
The role of Src kinase in the biology and pathogenesis of Acanthamoeba castellanii
Authors: Siddiqui Ruqaiyyah , Iqbal Junaid , Maugueret Marie-josée , Khan Naveed Ahmed 
Published: June 2012

AbstractBackgroundAcanthamoebaspecies are the causative agents of fatal granulomatous encephalitis in humans. Haematogenous spread is thought to be a primary step, followed by blood–brain barrier penetration, in the transmission ofAcanthmaoebainto the central nervous system, but the associated molecular mechanisms remain unclear. Here, we evaluated the role of Src, a non-receptor protein tyrosine kinase in the biology and pathogenesis ofAcanthamoeba.MethodsAmoebistatic and amoebicidal assays were performed by incubating amoeba in the presence of Src kinase-selective inhibitor, PP2 (4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine) and its inactive analog, PP3 (4-amino-7-phenylpyrazolo[3,4-d]pyrimidine). Using this inhibitor, the role of Src kinase inA. castellaniiinteractions withEscherichia coliwas determined. Zymographic assays were performed to study effects of Src kinase on extracellular proteolytic activities ofA. castellanii. The human brain microvascular endothelial cells were used to determine the effects of Src kinase onA. castellaniiadhesion to and cytotoxicity of host cells.ResultsInhibition of Src kinase using a specific inhibitor, PP2 (4-amino-5-(4 chlorophenyl)-7-(t-butyl)pyrazolo [3,4-d] pyrimidine) but not its inactive analog, PP3 (4-amino-7-phenylpyrazolo[3,4-d] pyrimidine), had detrimental effects on the growth ofA. castellanii(keratitis isolate, belonging to the T4 genotype). Interestingly, inhibition of Src kinase hampered the phagocytic ability ofA. castellanii, as measured by the uptake of non-invasive bacteria, but, on the contrary, invasion by pathogenic bacteria was enhanced. Zymographic assays revealed that inhibition of Src kinases reduced extracellular protease activities ofA. castellanii. Src kinase inhibition had no significant effect onA. castellaniibinding to and cytotoxicity of primary human brain microvascular endothelial cells, which constitute the blood–brain barrier.ConclusionsFor the first time, these findings demonstrated that Src kinase is involved inA. castellaniiproliferation, protease secretions and phagocytic properties. Conversely, invasion ofAcanthamoebaby pathogenic bacteria was stimulated by Src kinase inhibition.