bluuebunny/crossref_metadata_2025_split huggingface dataset:
Simultaneous determination of aripiprazole and its active metabolite, dehydroaripiprazole, in plasma by capillary electrophoresis combining on‐column field‐amplified sample injection and application in schizophrenia
Authors: Hwang Pei‐Lin , Wei Shao‐Yun , Yeh Hsin‐Hua , Ko Ju‐Yun , Chang Cheng‐Chen , Chen Su‐Hwei 
Published: July 2010

AbstractA sensitive high‐performance CZE combining on‐column field‐amplified sample injection (FASI) has been developed for simultaneous determination of aripiprazole and its active metabolite, dehydroaripiprazole, in human plasma. A sample pretreatment by means of liquid–liquid extraction (LLE) (diethyl ether) with subsequent quantitation by FASI‐CZE was used. The separation of aripiprazole and dehydroaripiprazole was performed using a BGE containing 150 mM phosphate buffer (pH 3.5) with 40% methanol and 0.02% PVA as a dynamic coating to reduce interaction of analytes with the capillary wall. Before sample loading, a methanol plug (0.3 psi, 6 s) was injected to permit FASI for stacking. The samples were injected electrokinetically (10 kV, 30 s) to introduce sample cations and the applied voltage was 20 kV with on‐column detection at 214 nm. Several parameters affecting the separation and sensitivity of the drug and its active metabolite were studied, including reconstitution solvent, organic modifier, pH and concentration of phosphate buffer. The linear ranges of the method for test drug and its active metabolite, in plasma using amlodipine as an internal standard, were over the range 5.0–100.0 ng/mL. One female volunteer (25 years old) was orally administered a single dose of 10 mg aripiprazole (Abilify®, Otsuka) and blood samples were drawn over a 60 h period for pharmacokinetic study. The method was also applied to monitor the concentration of aripiprazole and dehydroaripiprazole in plasma collected after oral administration of 20 or 30 mg aripiprazole (Abilify®, Otsuka) daily at steady state in one schizophrenic patient.
A study on using wide‐flange section web under out‐of‐plane flexure for passive energy dissipation
Authors: Benavent‐Climent Amadeo , Morillas Leandro , Vico Juan M. 
Published: July 2010

AbstractIt is not common to purposely subject the web of wide‐flange or I‐sections to out‐of‐plane bending. However, yielding the web under this loading condition can be a stable source of energy dissipation as the transition at the corner from the web to the flanges is smooth and weld‐free; this prevents stress concentrations causing premature failure and eliminates uncertainties and imperfections associated with welding. Further, short segments of wide‐flange or I‐sections constitute a simple and inexpensive energy dissipating device as minimum manufacturing is required and leftovers not useful for other structural purposes can be re‐utilized. This paper proposes a new type of seismic damper in the form of braces based on yielding the web of short length segments of wide‐flange or I‐shaped steel sections under out‐of‐plane bending. The hysteretic behavior and ultimate energy dissipation capacity is investigated via component tests under cyclic loads. The experimental results indicate that the damping device has stable restoring force characteristics and a high energy dissipation capacity. Based on these results, a simple hysteretic model for predicting the load–displacement curve of the seismic damper is proposed, along with a procedure for predicting its ultimate energy dissipation capacity and anticipating its failure under arbitrarily applied cyclic loads. The procedure considers the influence of the loading path on the ultimate energy dissipation capacity. Finally, shaking table tests on half‐scale structures are conducted to further verify the feasibility and effectiveness of the new damper, and to assess the accuracy of the hysteretic model and the procedure for predicting its failure. Copyright © 2010 John Wiley & Sons, Ltd.
Spatial characterization of salt accumulation in early stage limestone weathering using probe permeametry
Authors: Buj O. , Gisbert J. , McKinley J. M. , Smith B. 
Published: July 2010

AbstractThis research characterizes the weathering of natural building stone using an unsteady‐state portable probe permeameter. Variations between the permeability properties of fresh rock and the same rocks after the early stages of a salt weathering simulation are used to examine the effects of salt accumulation on spatial variations in surface rock permeability properties in two limestones from Spain. The Fraga and Tudela limestones are from the Ebro basin and are of Miocene age. Both stone types figure largely in the architectural heritage of Spain and, in common with many other building limestones, they are prone to physical damage from salt crystallization in pore spaces. To examine feedbacks associated with salt accumulation during the early stages of this weathering process, samples of the two stone types were subjected to simulated salt weathering under laboratory conditions using magnesium sulphate and sodium chloride at concentrations of 5% and 15%. Permeability mapping and statistical analysis (aspatial statistics and spatial prediction) before and after salt accumulation are used to assess changes in the spatial variability of permeability and to correlate these changes with salt movement, porosity change, potential rock deterioration and textural characteristics. Statistical analyses of small‐scale permeability measurements are used to evaluate the drivers for decay and hence aid the prediction of the weathering behaviour of the two limestones. Copyright © 2010 John Wiley & Sons, Ltd.
Optimization of operating conditions of Tunisian myrtle (Myrtus communis L.) essential oil extraction by a hydrodistillation process using a 24 complete factorial design
Authors: Ammar Ahlem Haj , Zagrouba Fethi , Romdhane Mehrez 
Published: July 2010

AbstractA 24 complete factorial design has been used to study the technological parameters that are responsible for the hydrodistillation process. After an appropriate choice of four critical variables, 16 experiments led to a mathematical model as a first degree polynomial presenting the response function (yield) in relation to the operating parameters. From the retained model, we were able to calculate the average response, the different effects and their interactions. The results showed that processing time, condensation flow and mass ratio plant material:water had significant effects (in absolute values) on myrtle essential oil yield (0.0350, −0.0421 and −0.0416, respectively), whereas granulometry presented a negligible effect (about −0.0084). The average essential oil yield extracted from 100 g myrtle leaves is 0.635%. Our study proved that experimental design methodology could efficiently be applied to characterize the analytical parameters affecting the hydrodistillation process and that it is an economical way of obtaining the maximum amount of information in a short period of time and with the smallest number of experiments. Copyright © 2010 John Wiley & Sons, Ltd.
An efficient implicit mesh‐less method for compressible flow calculations
Authors: Hashemi M. Y. , Jahangirian A. 
Published: July 2010

AbstractA dual‐time implicit mesh‐less scheme is presented for calculation of compressible inviscid flow equations. The Taylor series least‐square method is used for approximation of spatial derivatives at each node which leads to a central difference discretization. Several convergence acceleration techniques such as local time stepping, enthalpy damping and residual smoothing are adopted in this approach. The capabilities of the method are demonstrated by flow computations around single and multi‐element airfoils at subsonic, transonic and supersonic flow conditions. Results are presented which indicate good agreements with other reliable finite‐volume results. The computational time is considerably reduced when using the proposed mesh‐less method compared with the explicit mesh‐less and finite‐volume schemes using the same point distributions. Copyright © 2010 John Wiley & Sons, Ltd.
A home‐delivered intervention for depressed, cognitively impaired, disabled elders
Authors: Kiosses Dimitris N. , Teri Linda , Velligan Dawn I. , Alexopoulos George S. 
Published: July 2010

AbstractObjectiveProblem Adaptation Therapy (PATH) is a new home‐delivered intervention designed to reduce depression and disability in depressed, cognitively impaired, disabled elders. A new intervention is needed in this population as antidepressant treatment is effective in only a minority of these patients.MethodsPATH focuses on the patient's ecosystem, which includes the patient, the caregiver, and the home environment, to address the needs of depressed, cognitively impaired, disabled elders. It builds on the therapeutic framework of Problem Solving Therapy (PST), which has been efficacious in decreasing depression and disability in cognitively intact depressed elders. To address the needs of depressed elders with advanced cognitive impairment, PATH incorporates environmental adaptations and invites caregiver participation.ResultsTo illustrate the administration of PATH, two case studies with varying degrees of cognitive impairment and caregiver participation are presented. Both patients were administered 12 weeks of PATH at their home. At the end of treatment their depression and disability was significantly reduced.ConclusionsPATH is a new home‐delivered intervention for depressed elders with cognitive impairment and disability focusing on reducing depression and disability by employing environmental adaptations and inviting caregiver participation. This intervention may provide a treatment alternative for a population with limited success of antidepressant treatment. Copyright © 2010 John Wiley & Sons, Ltd.
Neurogenic marker abnormalities in the hippocampus in dementia with Lewy bodies
Authors: Johnson Mary , Ekonomou Antigoni , Hobbs Carl , Ballard Clive G. , Perry Robert H. , Perry Elaine K. 
Published: July 2010

AbstractDementia with Lewy bodies (DLB) is associated with alpha synuclein pathology and slowly progressive dementia. Progenitor abnormalities have previously been reported in the subventricular zone (SVZ) adjacent to the lateral ventricle. To evaluate changes in neural stem cells and progenitors in the hippocampal neurogenic niche, immunohistochemistry (IHC) using the neural stem cell markers Musashi 1, nestin, proliferating cell nuclear antigen (PCNA), doublecortin, and glial fibrillary acidic protein (GFAP) were examined in age‐matched control and DLB groups. Staining was quantified in the hippocampal SVZ, subgranular layer (SGL) and ependymal cell layer (EPL). There was a significant loss in DLB of Musashi 1 (P < 0.01) in all areas, an increase in PCNA in hippocampal SVZ (P = 0.01) and SGL (P = 0.05), and an increase in doublecortin in the hippocampal SVZ (P = 0.04) and EPL (P = 0.02). This is the first report of the changes in neurogenic markers in the hippocampal SVZ and EPL in DLB and may offer the potential for understanding disease pathology and in the devising of treatment. ©2010 Wiley‐Liss, Inc.
Chronic restraint stress in adolescence differentially influences hypothalamic‐pituitary‐adrenal axis function and adult hippocampal neurogenesis in male and female rats
Authors: Barha Cindy K. , Brummelte Susanne , Lieblich Stephanie E. , Galea Liisa A.M. 
Published: July 2010

AbstractPrevious studies have shown a relationship between adversity in adolescence and health outcomes in adulthood in a sex‐specific manner. Adolescence is characterized by major changes in stress‐responsive regions of the brain, including the hippocampus, the site of ongoing neurogenesis throughout the lifespan. Prepubertal male and female rats exhibit different acute reactions to chronic stress compared to adults, but less is known about whether these stress‐induced changes persist into adulthood. Therefore, in this study, we investigated the effects of chronic, intermittent stress during adolescence on basal corticosterone levels, dentate gyrus (DG) volume, and neurogenesis in the hippocampus of adult male and female Sprague‐Dawley rats. Adolescent male and female rats were either restrained for 1 h every other day for 3 weeks from postnatal days (PDs) 30–52 at unpredictable times or left undisturbed. All rats received a single injection of bromodeoxyuridine (BrdU; 200 mg/kg) in adulthood on PD70 and were perfused 3 weeks later. Brains were processed for Ki67 (endogenous marker of cell proliferation) and BrdU (to estimate effects on cell survival). In addition, blood samples were taken during the restraint stress period and in adulthood. Results show that males and females exhibit different corticosterone responses to chronic stress during adolescence and that only adult female rats exposed to stress during adolescence show higher basal corticosterone levels compared to nonstressed controls. Furthermore, stressed females showed a reduced number of proliferating and surviving cells in the DG in adulthood compared to nonstressed same‐sex controls. The majority of BrdU‐labeled cells were co‐labeled with NeuN, an endogenous marker of mature neurons, indicating that neurogenesis was decreased in the DG of adult female rats that had undergone chronic restraint stress in adolescence. Although male rats were more responsive to the chronic stress as adolescents showing higher corticosterone levels and reduced body weight, as adults they showed a slight increase in cellsurvival and no effect of adolescent stress on basal corticosterone levels. These results suggest that stress during adolescence can have effects on hypothalamic‐pituitary‐adrenal axis function and hippocampus plasticity in adulthood, particularly in female rats. ©2010 Wiley‐Liss,Inc.
Increase in polysialyltransferase gene expression following LTP in adult rat dentate gyrus
Authors: Guiraudie‐Capraz G. , Chaillan F.A. , Truchet B. , Franc J.L. , Mourre C. , Roman F.S. 
Published: July 2010

AbstractNeural cell adhesion molecule (NCAM) is frequently associated with polysialic acid (PSA), and its function is highly dependent on this polysialylation. PSA‐NCAM plays an important role in synaptic plasticity in the hippocampus. STX and PST are the enzymes responsible for NCAM polysialylation. We investigated whether unilateral long‐term potentiation (LTP) induction in vivo, in adult rat dentate gyrus (DG), triggered NCAM polysialylation by STX and PST produced in the hippocampus. We found that levels of STX and PST mRNA increased strongly since the early stage of hippocampal LTP and remained high during the maintenance of DG‐LTP for 4 h. This rapid increase in polysialyltransferase gene expression occurred in both the hippocampi, probably resulting from bilateral LTP induction by strong unilateral HFS. Thus, LTP triggers interhemispheric molecular changes in the hippocampal network. This study is the first to describe the effects of LTP induction and maintenance on polysialyltransferases in vivo. Our findings suggest that hippocampal synaptic remodeling requires NCAM polysialylation. ©2010 Wiley Periodicals, Inc.
Modelling heterogeneity and dynamics in the volatility of individual wages
Authors: Hospido L. 
Published: July 2010

AbstractThis paper presents a model for the heterogeneity and dynamics of the conditional mean and conditional variance of individual wages. A bias‐corrected likelihood approach, which reduces the estimation bias to a term of order 1/T2, is used for estimation and inference. The small‐sample performance of the proposed estimator is investigated in a Monte Carlo study. The simulation results show that the bias of the maximum likelihood estimator is substantially corrected for designs calibrated to the data used in the empirical analysis, drawn from the PSID. The empirical results show that it is important to account for individual unobserved heterogeneity and dynamics in the variance, and that the latter is driven by job mobility. The model also explains the non‐normality observed in log‐wage data. Copyright © 2010 John Wiley & Sons, Ltd.
Hydrogen peroxide regulates glucose‐regulated protein 78 expression via a cyclooxygenase‐2 dependent mechanism
Authors: Chen Ying‐ying , Zheng Ming‐zhi , Lv Ping‐ping , Hu Lin , Wang Lin‐lin , Shen Yue‐Liang 
Published: July 2010

AbstractThis study was designed to investigate the effect of hydrogen peroxide on the expression of endoplasmic reticulum stress marker glucose‐regulated protein 78 (GRP78) in endothelial cells and reveals the possible role of cyclooxygenase in this effect. The porcine endothelial cell line was cultured in 1640 medium. Western blot and immunocytochemistry were used to detect the expression of GRP78. The caspase‐12 activity was analyzed with the immune fluorescence method. The results showed that after the endothelial cells were incubated with 250 μM of hydrogen peroxide for 12 h, apoptosis increased, which was antagonized by the cyclooxygenase‐2 inhibitor nimesulide or the nonselective cyclooxygenase inhibitor aspirin, but not by the cyclooxygenase‐1 inhibitor piroxicam. The expression of GRP78 was induced in endothelial cells after exposure to hydrogen peroxide for 12 h. The overexpression of GRP78 was inhibited by nimesulide and aspirin, but not by piroxicam. There are no significant differences in caspase‐12 activity among all groups. The present study provides evidence that hydrogen peroxide induced GRP78 overexpression in endothelial cells by a mechanism involving cyclooxygenase‐2‐dependent pathway. © 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 24:279–285, 2010; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20336
Modification of the detrimental effect of TNF‐α on human endothelial progenitor cells by fasudil and Y27632
Authors: Balestrieri Maria Luisa , Giovane Alfonso , Milone Lara , Felice Francesca , Fiorito Carmela , Crudele Valeria , Esposito Annaclaudia , Rossiello Raffaele , Minucci Pellegrino Biagio , Farzati Bartolomeo , Servillo Luigi , Napoli Claudio 
Published: July 2010

AbstractExposure of human endothelial progenitor cells (EPCs) to tumor necrosis factor‐α (TNF‐α) reduced their number and biological activity. Yet, signal transduction events linked to TNF‐α action are still poorly understood. To address this issue, we examined the possible effect of fasudil and Y27632, two inhibitors of Rho kinase pathway, which is involved in endothelial dysfunction, atherosclerosis, and in‐ flammation. Results demonstrated that incubation with fasudil starting from 50 μM but not Y27632 determined a dose‐dependent improvement of EPC number during exposure to TNF‐α (P < 0.05 vs. TNF‐α alone). Analysis of the signal transduction pathway activated by TNF‐α revealed that the increased expression of p‐p38 was not significantly altered by fasudil. Instead, fasudil blocked the TNF‐α induced phosphorylation of Erk1/2 (P < 0.05 vs. TNF‐α) as well as the inhibitor of Erk1/2‐specific phosphorylated form, i.e., PD98059 (P < 0.05 vs. TNF‐α). These results were confirmed by analysis of these kinases by confocal microscopy. Finally, 2D‐DIGE and MALDI‐TOF/TOF analysis of EPCs treated with fasudil revealed increased expression levels of an actin‐related protein and an adenylyl cyclase associated protein and decreased expression levels of proteins related to radical scavenger and nucleotide metabolism. These findings suggest that fasudil positively affects EPC number and that other major signals might take part to this complex pathway. © 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 24:351–360, 2010; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20345
Proteomic analysis of urine in rats chronically exposed to fluoride
Authors: Kobayashi Claudia Ayumi Nakai , Leite Aline de Lima , Silva Thelma Lopes da , Santos Lucilene Delazari dos , Nogueira Fábio César Sousa , Santos Keity Souza , Oliveira Rodrigo Cardoso de , Palma Mario Sérgio , Domont Gilberto Barbosa , Buzalaf Marília Afonso Rabelo 
Published: July 2010

AbstractUrine is an ideal source of materials to search for potential disease‐related biomarkers as it is produced by the affected tissues and can be easily obtained by noninvasive methods. 2‐DE‐based proteomic approach was used to better understand the molecular mechanisms of injury induced by fluoride (F−) and define potential biomarkers of dental fluorosis. Three groups of weanling male Wistar rats were treated with drinking water containing 0 (control), 5, or 50 ppm F− for 60 days (n = 15/group). During the experimental period, the animals were kept individually in metabolic cages, to analyze the water and food consumption, as well as fecal and urinary F− excretion. Urinary proteome profiles were examined using 2‐DE and Colloidal Coomassie Brilliant Blue staining. A dose‐response regarding F− intake and excretion was detected. Quantitative intensity analysis revealed 8, 11, and 8 significantly altered proteins between control vs. 5 ppm F−, control vs. 50 ppm F− and 5 ppm F− vs. 50 ppm F− groups, respectively. Two proteins regulated by androgens (androgen‐regulated 20‐KDa protein and α‐2μ‐globulin) and one related to detoxification (aflatoxin‐B1‐aldehyde‐reductase) were identified by MALDI‐TOF‐TOF MS/MS. Thus, proteomic analysis can help to better understand the mechanisms underlying F− toxicity, even in low doses. © 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:8–14 2011; View this article online at wileyonlinelibrary.com. DOI 10.1002/jbt.20353
The odontogenic ameloblast‐associated protein (ODAM) cooperates with RUNX2 and modulates enamel mineralization via regulation of MMP‐20
Authors: Lee Hye‐Kyung , Lee Dong‐Seol , Ryoo Hyun‐Mo , Park Jong‐Tae , Park Su‐Jin , Bae Hyun‐Sook , Cho Moon‐Il , Park Joo‐Cheol 
Published: July 2010

AbstractWe have previously reported that the odontogenic ameloblast‐associated protein (ODAM) plays important roles in enamel mineralization through the regulation of matrix metalloproteinase‐20 (MMP‐20). However, the precise function of ODAM in MMP‐20 regulation remains largely unknown. The aim of the present study was to uncover the molecular mechanisms responsible for MMP‐20 regulation. The subcellular localization of ODAM varies in a stage‐specific fashion during ameloblast differentiation. During the secretory stage of amelogenesis ODAM was localized to both the nucleus and cytoplasm of ameloblasts. However, during the maturation stage of amelogenesis, ODAM was observed in the cytoplasm and at the interface between ameloblasts and the enamel layer, but not in the nucleus. Secreted ODAM was detected in the conditioned medium of ameloblast‐lineage cell line (ALC) from days 14 to 21, which coincided with the maturation stage of amelogenesis. Interestingly, the expression of Runx2 and nuclear ODAM correlated with MMP‐20 expression in ALC. We therefore examined whether ODAM cooperates with Runx2 to regulate MMP‐20 and modulate enamel mineralization. Increased expression of ODAM and Runx2 augmented MMP‐20 expression, and Runx2 expression enhanced expression of ODAM, although overexpression of ODAM did not influence Runx2 expression. Conversely, loss of Runx2 in ALC decreased ODAM expression, resulting in down‐regulation of MMP‐20 expression. Increased MMP‐20 expression accelerated amelogenin processing during enamel mineralization. Our data suggest that Runx2 regulates the expression of ODAM and that nuclear ODAM serves an important regulatory function in the mineralization of enamel through the regulation of MMP‐20 apart from a different, currently unidentified, function of extracellular ODAM. J. Cell. Biochem. 111: 755–767, 2010. © 2010 Wiley‐Liss, Inc.