bluuebunny/crossref_metadata_2025_split huggingface dataset:
A novel 7‐azaisoindigo derivative‐induced cancer cell apoptosis and mitochondrial dysfunction mediated by oxidative stress
Authors: Xu Jing‐jing , Dai Xiao‐min , Liu Hai‐liang , Guo Wen‐jie , Gao Jing , Wang Chao‐hui , Li Wen‐bin , Yao Qi‐zheng 
Published: September 2010

AbstractThis research focused on a novel 7‐azaisoindigo derivative [namely N1‐(n‐butyl)‐7‐azaisoindigo, 7‐AI‐b], and investigated its molecular antitumor mechanism by exploring the means of cell death and the effects on mitochondrial function. 7‐AI‐b inhibited cancer cell proliferation in a dose‐ and time‐dependent way. The morphological and nuclei changes in H2B‐GFP‐labeled HeLa cells were observed using a live cell system. The results suggested that cell death induced by 7‐AI‐b is closely related to apoptosis. 7‐AI‐b induced release of cytochrome C from mitochondria to cytosol and activation of caspase‐3, showing that the apoptosis is mediated by the mitochondrial pathway. Furthermore, our data indicated that 7‐AI‐b triggers apoptosis through reactive oxygen species (ROS): cellular ROS levels were increased after 3 h exposure of 7‐AI‐b, which was reversed by the ROS scavenger N‐acetyl‐l‐cysteine. As a consequence, 7‐AI‐b‐mediated cell death, mitochondrial transmembrane potential collapse and ATP level were partly blocked by N‐acetyl‐l‐cysteine. Further study showed that 7‐AI‐b could induce mitochondrial dysfunction: collapse of the mitochondrial transmembrane potential and reduction of intracellular ATP level. In summary, the novel synthesized 7‐AI‐b was demonstrated to be effective in killing cancer cells via an ROS‐promoted and mitochondria‐ and caspase‐dependent apoptotic pathway. Copyright © 2010 John Wiley & Sons, Ltd.
Anoxia, acidosis, and intergenic interactions selectively regulate methionine sulfoxide reductase transcriptions in mouse embryonic stem cells
Authors: Zhang Chi , Jia Pingping , Jia Yuanyuan , Li Yuejin , Webster Keith A. , Huang Xupei , Achary Mohan , Lemanski Sharon L. , Lemanski Larry F. 
Published: September 2010

AbstractMethionine sulfoxide reductases (Msr) belong to a gene family that contains one MsrA and three MsrBs (MsrB1, MsrB2, and MsrB3). We have identified all four of the genes that are expressed in mouse embryonic stem cell cultures. The vital cellular functions of the Msr family of genes are to protect cells from oxidative damage by enzymatically reducing the oxidized sulfide groups of methionine residues in proteins from the sulfoxide form (SO) back to sulfide thus restoring normal protein functions as well as reducing intracellular reactive oxygen species (ROS). We have performed studies on the Msr family genes to examine the regulation of gene expression. Our studies using real‐time RT‐PCR and Western blotting have shown that expression levels of the four Msr family genes are under differential regulation by anoxia/reoxygenation treatment, acidic culture conditions and interactions between MsrA and MsrB. Results from these in vitro experiments suggest that although these genes function as a whole in oxidative stress protection, each one of the Msr genes could be responsive to environmental stimulants differently at the tissue level. J. Cell. Biochem. 112: 98–106, 2011. © 2010 Wiley‐Liss, Inc.
Red wine may be used in the therapy of myocarditis
Authors: Chen Chun‐Juan , Yu Wei , Wang Wei 
Published: September 2010

AbstractMyocarditis is one of the most commonly cardiovascular diseases in clinical practice, but the treatment is always limited at present. Considering the multifactorial etiology of myocarditis, a novel therapeutic agent with multi‐bioactivties should be presented. Red wine has been recognized as a favorable natural medicine against a large number of pathologic conditions. Recent results indicate that red wine could effectively decrease inflammatory factors secretion, reduce the migration of neutrophils, antagonize oxidation, and regulate immunity. By these bioactivities of anti‐inflammation, anti‐oxidation, and immunomodulation, red wine may be an effective therapeutic candidate to manage the symptoms and prevent the recurrence of myocarditis. J. Cell. Biochem. 111: 808–810, 2010. © 2010 Wiley‐Liss, Inc.
Evaluation of fetal nasofrontal angle in the second trimester in normal pregnancies
Authors: Öztürk Hasan , İpek Ali , Tan Sinan , Yener Öztürk Şenay , Keskin Suat , Kurt Aydın , Arslan Halil 
Published: September 2010

AbstractPurpose:The nasal bone is one of the important fetal structures to be radiologically evaluated in the first and second trimesters. In this study, we aimed to evaluate the nasal root and to determine the normal range of the naso‐frontal angle, which may potentially be used as a predictive or diagnostic parameter in the diagnosis of the above‐mentioned syndromes or other congenital abnormalities with associated nasal features using obstetric ultrasonography in normal fetuses.Methods:We evaluated the naso‐frontal angle using transabdominal ultrasonography in 195 healthy fetuses between the 18th and 21st weeks of gestation. The measurements were performed with the fetal neck in mild flexion and three echogenic lines (nasal end, skin on the nasal bone, nasal bone) visualized.Results:The minimum, maximum and mean naso‐frontal angle values were 110°, 143° and 128°, respectively, between the 18th and 21st weeks of gestation. Linear regression analysis showed no association between NFA and gestational week (Beta = 0.020 p = 0.786).Conclusions:The normal range of NFA between the 18th and 21st weeks of gestation was determined in this study. The association between NFA measurements and various congenital anomalies or fetal outcomes remains to be investigated. © 2010 Wiley Periodicals, Inc. J Clin Ultrasound, 2011
Clinically significant thromboembolic disease in orthopedic oncology: An analysis of 986 patients treated with low‐molecular‐weight heparin
Authors: Ruggieri Pietro , Montalti Maurizio , Pala Elisa , Angelini Andrea , Calabrò Teresa , Errani Costantino , Mercuri Mario 
Published: September 2010

AbstractBackground and ObjectivesThromboembolic risk in orthopedic oncology is high due to several factors. The aim of this study was to assess clinically significant thromboembolic disease in 986 patients operated on with a prosthetic reconstruction of the lower limbs after the resection of bone tumors and prophylactically treated with low‐molecular‐weight heparin (LMWH).MethodsBetween 1983 and 2006, 986 patients had uncemented megaprostheses after a resection of the lower limbs for bone tumors. Antithromboembolic prophylaxis was always administered with LMWH from the immediate postoperative time until the time of complete weight‐bearing. Phlebographies and vascular ecodoppler were not performed postoperatively on a regular basis. Patients were followed in the clinic with imaging studies for several years (lower limb CT, MRI, CT of the chest for malignant tumors). The diagnosis of symptomatic venous thromboembolism (VTE) was established or excluded on clinical evidence and MRI study.ResultsAmong the 986 cases treated, only 11 patients (1.1%) showed a major thromboembolic event confirmed clinically and through imaging. One of these patients died with pulmonary embolism a few days after surgery. Two cases of thromboembolism occurred in patients with a vascular bypass.ConclusionsDespite general oncologic and orthopedic factors favoring VTE, the clinical occurrence of this event was extremely low in this series, probably due to a consistent and careful prophylaxis, prolonged until the time of complete weight‐bearing. J. Surg. Oncol. 2010;102:375–379. © 2010 Wiley‐Liss, Inc.
The impact of preoperative chemoradiotherapy on advanced low rectal cancer
Authors: Kao Ping‐Sheng , Chang Shih‐Ching , Wang Ling‐Wei , Lee Rheun‐Chuan , Liang Wen‐Yi , Lin Tzu‐Chen , Chen Wei‐Shone , Jiang Jeng‐Kai , Yang Shung‐Haur , Wang Huann‐Sheng , Lin Jen‐Kou 
Published: September 2010

AbstractBackgroundPreoperative chemoradiotherapy (CCRT) followed by radical resection is an option for advanced low rectal cancer. This study was aimed to clarify the impact of CCRT on patients' outcome.Patients and MethodsOne hundred thirty‐six patients with rectal cancer (<10 cm from anal verge) were enrolled prospectively between July 2000 and December 2004. The preoperative clinical stage was T3, T4, or node‐positive disease. Sixty‐nine and 67 patients underwent surgery with and without preoperative CCRT, respectively. The regimen of pre‐op CCRT was a radiation dosage of 45 Gy in 20 fractions and oral tegafur‐uracil (UFUR) and leucovorin.ResultsThere was no statistical difference in the preserved anorectal function between two groups after 5 years of follow‐up (62.3% vs. 47.8%; P = 0.125). The 5‐year overall survival and disease‐free survival (DFS) percentage were 88.4% and 76.8% for patients with preoperative CCRT, and 65.7% and 58.2% for patients without CCRT, respectively. Patients with preoperative CCRT had a higher overall survival rate and DFS (P = 0.001 and 0.015).ConclusionsIn patients with advanced low rectal cancer, preoperative CCRT followed by radical surgery significantly improved overall survival and DFS compared with surgery alone. The effect of sphincter preservation with preoperative CCRT is questionable. J. Surg. Oncol. 2010;102:771–777. © 2010 Wiley‐Liss, Inc.
Macrophage migration inhibitory factor contributes angiogenesis by up‐regulating IL‐8 and correlates with poor prognosis of patients with primary nasopharyngeal carcinoma
Authors: Liao Bing , Zhong Bi‐ling , Li Zhi , Tian Xiao‐ying , Li Yang , Li Bin 
Published: September 2010

AbstractBackground and ObjectivesWe aim at the association of macrophage migration inhibitory factor (MIF) with neovascularization and survival of nasopharyngeal carcinoma (NPC), and determine whether MIF is a valuable prognostic predictor for NPC patients.MethodsOne hundred and forty one cases of NPC and 25 normal tissues of nasopharynx were collected. The expression of MIF and interleukin 8 (IL‐8) was evaluated in tissues microarray by immunostaining. Intratumoral microvessel density (IMD) in relation to immunostainings and clinicopathological factors were analyzed statistically as well as the follow‐up data of patients.ResultsHigh‐expression of both MIF (69.5%) and IL‐8 (56.0%) were significantly associated with increased microvessels and lymph node metastasis. High‐expression of MIF, IL‐8 and higher level of IMD were correlated with either patients' overall survival or disease‐specific survival in univariate analysis, but only angiogenesis and lymph node status exhibited in relation to survival of patients as independent prognostic factor of NPC by multivariate analysis. In addition, high‐expression of MIF and higher level of IMD were closely associated with locoregional failure of NPC patients.ConclusionsMIF may contribute to lymph node metastasis in NPC by inducing angiogenesis through the way of upregulation of IL‐8 expression in an autocrine EBV‐independent pathway. J. Surg. Oncol. 2010;102:844–851. © 2010 Wiley‐Liss, Inc.
Evaluation of stationary phases and gas chromatographic detectors for determination of amines in water
Authors: Jurado‐Sánchez Beatriz , Ballesteros Evaristo , Gallego Mercedes 
Published: September 2010

AbstractFor the first time, a systematic overview deals with the advantages and disadvantages of several stationary phases (polar and non‐polar) and gas chromatographic detectors (flame ionization detector, nitrogen–phosphorus detector and MS) for the determination of 27 amines (aliphatic and aromatic amines and N‐nitrosamines) in water samples. To increase sensitivity (250 mL of sample was eluted with 150 μL of solvent) and matrix elimination, an automatic SPE system was employed prior to GC determination. The best results in terms of resolution and retention times were achieved using a column coated with 5% phenyl‐dimethylpolysiloxane (DB‐5). Capacity factor (k) values for the 27 amines increased with the rise in the polarity of the stationary phase, ranging from 3.0–27.7 and 2.2–14.4 for polar (polyethylene glycol) and non‐polar (DB‐5) columns, respectively. The detection limits of the method were 0.9–9 μg/L for flame ionization detector, 8–95 ng/L for nitrogen–phosphorus detector and 0.2–6.3 ng/L for MS. The precision was similar for the three detectors (RSD, 3.7–6.0%). The GC‐MS method was applied with a high degree of accuracy and precision to determine amines in real samples including tap, river, pond, well, swimming pool and wastewaters.
HPLC enantioseparation of α,α‐diphenyl‐2‐pyrrolidinemethanol and methylphenidate using a chiral fluorescent derivatization reagent and its application to the analysis of rat plasma
Authors: Inagaki Shinsuke , Taniguchi Sayuri , Hirashima Haruo , Higashi Tatsuya , Min Jun Zhe , Kikura‐Hanajiri Ruri , Goda Yukihiro , Toyo'oka Toshimasa 
Published: September 2010

AbstractEnantioseparation of α,α‐diphenyl‐2‐pyrrolidinemethanol (D2PM) and methylphenidate (MPH; Ritalin®) using (R)‐(−)‐4‐(N,N‐dimethylaminosulfonyl)‐7‐(3‐isothiocyanatopyrrolidin‐1‐yl)‐2,1,3‐benzoxadiazole as the chiral derivatization reagent has been achieved for the first time, and a simple, reliable detection method using HPLC with fluorescence detection has been developed. D2PM and MPH have been derivatized with (R)‐(−)‐4‐(N,N‐dimethylaminosulfonyl)‐7‐(3‐isothiocyanatopyrrolidin‐1‐yl)‐2,1,3‐benzoxadiazole at 55°C for 15 min. The derivatives of D2PM and MPH have been separated, completely and rapidly, using a reversed‐phase system within 16 min (resolution factor (Rs)=1.60 and 2.53, respectively). The detection limits of (R)‐ and (S)‐D2PM were found to be 6.8 and 13 ng/mL, respectively, and those of D‐ and L‐threo‐MPH were 61 and 66 ng/mL, respectively (S/N=3). The proposed method was successfully applied to the analysis of rat plasma, where the rats were separately dosed with D2PM and MPH (Ritalin).
Characterization of Mixtures. Part 2: QSPR Models for Prediction of Excess Molar Volume and Liquid Density Using Neural Networks
Authors: Ajmani Subhash , Rogers Stephen C. , Barley Mark H. , Burgess Andrew N. , Livingstone David J. 
Published: September 2010

AbstractIn our earlier work, we have demonstrated that it is possible to characterize binary mixtures using single component descriptors by applying various mixing rules. We also showed that these methods were successful in building predictive QSPR models to study various mixture properties of interest. Here in, we developed a QSPR model of an excess thermodynamic property of binary mixtures i.e. excess molar volume (VE). In the present study, we use a set of mixture descriptors which we earlier designed to specifically account for intermolecular interactions between the components of a mixture and applied successfully to the prediction of infinite‐dilution activity coefficients using neural networks (part 1 of this series). We obtain a significant QSPR model for the prediction of excess molar volume (VE) using consensus neural networks and five mixture descriptors. We find that hydrogen bond and thermodynamic descriptors are the most important in determining excess molar volume (VE), which is in line with the theory of intermolecular forces governing excess mixture properties. The results also suggest that the mixture descriptors utilized herein may be sufficient to model a wide variety of properties of binary and possibly even more complex mixtures.
Multicomponent T2* mapping of knee cartilage: Technical feasibility ex vivo
Authors: Qian Yongxian , Williams Ashley A. , Chu Constance R. , Boada Fernando E. 
Published: September 2010

AbstractDisorganization of collagen fibers is a sign of early‐stage cartilage degeneration in osteoarthritic knees. Water molecules trapped within well‐organized collagen fibrils would be sensitive to collagen alterations. Multicomponent effective transverse relaxation (T2*) mapping with ultrashort echo time acquisitions is here proposed to probe short T2 relaxations in those trapped water molecules. Six human tibial plateau explants were scanned on a 3T MRI scanner using a home‐developed ultrashort echo time sequence with echo times optimized via Monte Carlo simulations. Time constants and component intensities of T2* decays were calculated at individual pixels, using the nonnegative least squares algorithm. Four T2*‐decay types were found: 99% of cartilage pixels having mono‐, bi‐, or nonexponential decay, and 1% showing triexponential decay. Short T2* was mainly in 1‐6 ms, while long T2* was ∼22 ms. A map of decay types presented spatial distribution of these T2* decays. These results showed the technical feasibility of multicomponent T2* mapping on human knee cartilage explants. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.
Estimation of perfusion and arterial transit time in myocardium using free‐breathing myocardial arterial spin labeling with navigator‐echo
Authors: Wang Danny J. J. , Bi Xiaoming , Avants Brian B. , Meng Tongbai , Zuehlsdorff Sven , Detre John A. 
Published: September 2010

AbstractArterial spin labeling (ASL) provides noninvasive measurement of tissue blood flow, but sensitivity to motion has limited its application to imaging of myocardial blood flow. Although different cardiac phases can be synchronized using electrocardiography triggering, breath holding is generally required to minimize effects of respiratory motion during ASL scanning, which may be challenging in clinical populations. Here a free‐breathing myocardial ASL technique with the potential for reliable clinical application is presented, by combining ASL with a navigator‐gated, electrocardiography‐triggered TrueFISP readout sequence. Dynamic myocardial perfusion signals were measured at multiple delay times that allowed simultaneous fitting of myocardial blood flow and arterial transit time. With the assist of a nonrigid motion correction program, the estimated mean myocardial blood flow was 1.00 ± 0.55 mL/g/min with a mean transit time of ∼400 msec. The intraclass correlation coefficient of repeated scans was 0.89 with a mean within subject coefficient of variation of 22%. Perfusion response during mild to moderate stress was further measured. The capability for noninvasive, free‐breathing assessment of myocardial blood flow using ASL may offer an alternative approach to first‐pass perfusion MRI for clinical evaluation of patients with coronary artery disease. Magn Reson Med, 2010. © 2010 Wiley‐Liss, Inc.
Robust spatially selective excitation using radiofrequency pulses adapted to the effective spatially encoding magnetic fields
Authors: Schneider Johannes T. , Haas Martin , Ruhm Wolfgang , Hennig Jürgen , Ullmann Peter 
Published: September 2010

AbstractMultidimensional spatially selective excitation (SSE) has stimulated a variety of useful applications in magnetic resonance imaging and magnetic resonance spectroscopy, which have regained considerable interest after the recent introduction of parallel excitation. For SSE, radiofrequency pulses are designed specifically for certain time‐courses of spatially encoding magnetic fields (SEM) which are applied simultaneously with the radiofrequency pulses. However, experimental imperfections of gradient‐systems and undesired SEM field contributions often prevent the correct co‐action of radiofrequency pulses and gradient‐waveforms and therefore degrade the fidelity of excitation patterns, especially for parallel excitation. To cope with such imperfections, a classical measurement of k‐space‐trajectories can be performed followed by an adaptation of the SSE‐pulses. However, this method is limited to linear SEM field distributions, which are describable in the k‐space‐formalism. Hence, this work presents a more sophisticated method consisting in a spatially resolved measurement of the temporal phase evolution of the transverse magnetization. This exhaustive phase information can be incorporated into pulse‐design algorithms to compensate even for undesired spatially nonlinear, dynamic SEM field contributions. Both approaches are assessed in various experimental scenarios and individual benefits and limitations are discussed. The adaptation of SSE‐pulses to experimentally achieved calibration data turned out to be very beneficial, and especially the novel spatially resolved method exhibited high potential for robust SSE even in adverse experimental setups. Magn Reson Med, 2011. © 2010 Wiley‐Liss, Inc.
Women's attitudes to the successive decisions possibly involved in prenatal screening for Down syndrome: how consistent with their actual decisions?
Authors: Seror Valerie , Ville Yves 
Published: September 2010

AbstractObjectivesTo investigate women's attitudes to successive decisions possibly involved in Down syndrome screening (i.e. about ultrasound and biochemical screening, invasive testing, and termination of pregnancy) and to compare attitudes and actual decisions.MethodsFour hundred women were invited to complete a self‐administered questionnaire within 3 days postpartum. A hierarchical cluster analysis identified respondents with similar attitudes, and logistic regressions assessed the effects of factors relating to decision‐making on attitudes.ResultsThree hundred and ninety‐one women returned the questionnaire. Three clusters of women were identified. Two clusters comprised respondents stating that decision‐making should be based on attitudes to either termination of pregnancy (32.8%) or invasive testing‐related miscarriage (30.2%). The third cluster (37.0%) considered that obtaining information about pregnancy should be the main objective when deciding about biochemical screening or invasive testing. Multivariate logistic regressions showed some consistency between attitudes and previous decisions.Practice implicationsProviding information and eliciting women's attitudes towards the sequence of decisions possibly involved in Down syndrome screening could help them make their decisions about screening and/or invasive testing in line with their attitudes. Copyright © 2010 John Wiley & Sons, Ltd.
Sex—Differences on self‐medication in Spain
Authors: Carrasco‐Garrido Pilar , Hernández‐Barrera Valentín , López de Andrés Ana , Jiménez‐Trujillo Isabel , Jiménez‐García Rodrigo 
Published: September 2010

AbstractPurposeThis study mainly aimed at describing the factors associated with self‐medicated consumption of drugs in Spain from a gender perspective.MethodsDescriptive, cross‐sectional study covering the Spanish adult population, using data drawn from the 2006 and 2007 Spanish National Health Survey (SNHS). A total of 20 738 subjects were analyzed. The independent variables were sociodemographic, lifestyle, and health‐related, and the dependent variable was self‐medicated drug use. Using logistic multivariate regression models we have estimated the independent effect of each of these variables on the self‐medicated consumption. Two models were generated, one for female and one for male.ResultsThe 20.17% of all Spaniards indulge in self‐medication. The prevalence of self‐medication was 16.93% (2715) for women and 14.46% (1469) for men (p < 0.05). The variables that were independently and significantly associated with a greater probability of self‐medicated consumption in women were: lower age; consumption of alcohol; smoking habit. Among men, self‐medication is associated with nationality (immigrants were more likely to self‐medicate), income, and alcohol consumption.ConclusionsThe prevalence of self‐medicated drug use is higher in women than men. In our population, the influence of unhealthy lifestyles, such as alcohol and tobacco consumption, are related to a higher likelihood of self‐medication Copyright © 2010 John Wiley & Sons, Ltd.