bluuebunny/crossref_metadata_2025_split huggingface dataset:
Treating the aged in rural communities: the application of cognitive‐behavioral therapy for depression
Authors: Crowther Martha R. , Scogin Forrest , Johnson Norton Misti 
Published: March 2010

AbstractMany rural communities are experiencing an increase in their older adult population. Older adults who live in rural areas typically have fewer resources and poorer mental and physical health status than do their urban counterparts. Depression is the most prevalent mental health problem among older adults, and 80% of the cases are treatable. Unfortunately, for many rural elders, depressive disorders are widely under‐recognized and often untreated or undertreated. Psychotherapy is illustrated with the case of a 65‐year‐old rural married man whose presenting complaint was depressive symptoms after a myocardial infarction and loss of ability to work. The case illustrates that respect for rural elderly clients' deeply held beliefs about gender and therapy, coupled with an understanding of their limited resources, can be combined with psychoeducational and therapeutic interventions to offer new options. © 2010 Wiley Periodicals, Inc. J Clin Psychol: In Session 66:1–11, 2010.
Estrogen regulated expression of the p21Waf1/Cip1 gene in estrogen receptor positive human breast cancer cells
Authors: Mandal Soma , Davie James R. 
Published: March 2010

AbstractThe cyclin‐dependent kinase inhibitor protein p21Waf1/Cip1 is a potent tumor suppressor. Here, we demonstrate that estradiol regulates the p21Waf1/Cip1 gene. Estradiol induces p21Waf1/Cip1 mRNA expression within 30–60 min independent of new protein synthesis in the estrogen receptor α (ERα) positive human breast cancer cell line MCF‐7. Similar to other estradiol responsive promoters, the p21Waf1/Cip1 upstream promoter region has several estrogen response element (ERE) half‐sites nestled in AP‐1 binding sites, which are positioned upstream to Sp1 binding sites. Using the chromatin immunoprecipitation (ChIP) assay, we show that estradiol stimulation resulted in the recruitment of transcription factors ERα, Sp1, and Sp3 to the p21Waf1/Cip1 upstream promoter element. The Sp1 inhibitor mithramycin A abrogated Sp1, and to a lesser extent Sp3 binding, and markedly reduced the estradiol stimulated p21Waf1/Cip1 gene expression. However, ERα binding was not affected in the mithramycin A and estradiol treated cells. On closer examination of the half‐site ERE/AP‐1 sites upstream to the Sp1 sites in a separate ChIP experiment, we found a pronounced association of ERα upon estradiol treatment compared to almost negligible binding of Sp1 or Sp3. Together these studies provide evidence that ERα is recruited to the half‐site ERE/AP‐1 sites in the p21Waf1/Cip1 upstream promoter element. Although Sp1/Sp3 is not involved in the recruitment of ERα to the promoter, Sp1 is necessary for estrogen‐induced p21Waf1/Cip1 promoter activity. J. Cell. Physiol. 224:28–32, 2010 © 2010 Wiley‐Liss, Inc.
Epitope mapping of function‐blocking monoclonal antibody CM6 suggests a “weak” integrin binding site on the laminin‐332 LG2 domain
Authors: Yamashita Hironobu , Shang Meiling , Tripathi Manisha , Jourquin Jerome , Georgescu Walter , Liu Shanshan , Weidow Brandy , Quaranta Vito 
Published: March 2010

AbstractLaminin‐332 (Ln‐332) is an extracellular matrix molecule that regulates cell adhesion, spreading, and migration by interaction with cell surface receptors such as α3β1 and α6β4. Previously, we developed a function‐blocking monoclonal antibody against rat Ln‐332, CM6, which blocks hemidesmosome assembly induced by Ln‐332‐α6β4 interactions. However, the location of its epitope on Ln‐332 has remained unclear. In this study, we show that the CM6 epitope is located on the laminin G‐like (LG)2 module of the Ln‐332 α3 chain. To specify the residues involved in this epitope, we produced a series of GST‐fused α3 LG2 mutant proteins in which rat‐specific acids were replaced with human acids by a site‐directed mutagenesis strategy. CM6 reactivity against these proteins showed that CM6 binds to the 1089NERSVR1094 sequence of rat Ln‐332 LG2 module. In a structural model, this sequence maps to an LG2 loop sequence that is exposed to solvent according to predictions, consistent with its accessibility to antibody. CM6 inhibits integrin‐dependent cell adhesion on Ln‐332 and inhibits cell spreading on both Ln‐332 and recombinant LG2 (rLG2; but not rLG3), suggesting the presence of an α3β1 binding site on LG2. However, we were unable to show that rLG2 supports adhesion in standard assays, suggesting that LG2 may contain a “weak” integrin binding site, only detectable in spreading assays that do not require washes. These results, together with our previous findings, indicate that binding sites for α3β1 and α6β4 are closely spaced in the Ln‐332 LG domains where they regulate alternative cell functions, namely adhesion/migration or hemidesmosome anchoring. J. Cell. Physiol. 223:541–548, 2010. © 2010 Wiley‐Liss, Inc.
Promotion of cell spreading and migration by vascular endothelial‐protein tyrosine phosphatase (VE‐PTP) in cooperation with integrins
Authors: Mori Munemasa , Murata Yoji , Kotani Takenori , Kusakari Shinya , Ohnishi Hiroshi , Saito Yasuyuki , Okazawa Hideki , Ishizuka Tamotsu , Mori Masatomo , Matozaki Takashi 
Published: March 2010

AbstractVascular endothelial‐protein tyrosine phosphatase (VE‐PTP) is a receptor‐type protein tyrosine phosphatase with a single catalytic domain in its cytoplasmic region and multiple fibronectin type III‐like domains in its extracellular region. VE‐PTP is expressed specifically in endothelial cells and is implicated in regulation of angiogenesis. The molecular basis for such regulation by VE‐PTP has remained largely unknown, however. We now show that forced expression of VE‐PTP promoted cell spreading as well as formation of lamellipodia and filopodia in cultured fibroblasts plated on fibronectin. These effects of VE‐PTP on cell morphology required its catalytic activity as well as activation of integrins and Ras. In addition, VE‐PTP‐induced cell spreading and lamellipodium formation were prevented by inhibition of Src family kinases or of Rac or Cdc42. Indeed, forced expression of VE‐PTP increased the level of c‐Src phosphorylation at tyrosine‐416. Moreover, the VE‐PTP‐induced changes in cell morphology were suppressed by expression of dominant negative forms of FRG or Vav2, both of which are guanine nucleotide exchange factors for Rho family proteins and are activated by tyrosine phosphorylation. Forced expression of VE‐PTP also enhanced fibronectin‐dependent migration of cultured fibroblasts. Conversely, depletion of VE‐PTP by RNA interference in human umbilical vein endothelial cells or mouse endothelioma cells inhibited cell spreading on fibronectin. These results suggest that VE‐PTP, in cooperation with integrins, regulates the spreading and migration of endothelial cells during angiogenesis. J. Cell. Physiol. 224:195–204, 2010 © 2010 Wiley‐Liss, Inc.
Esterification activities of non‐commercial lipases after pre‐treatment in pressurized propane
Authors: Kuhn Graciele , Marangoni Marluci , Freire Denise M. G. , Soares Valéria Ferreira , de Godoy Mateus Gomes , de Castro Aline Machado , Luccio Marco Di , Treichel Helen , Mazutti Marcio A. , Oliveira Débora , Oliveira J. Vladimir 
Published: March 2010

AbstractBACKGROUND: The objective of this work was to assess the influence of propane pre‐treatment on the esterification activities of two non‐commercial lipases from Penicillium simplicissimum and Aspergillus parasiticus in the lyophilized and immobilized forms, evaluating the effect of pressure, exposure time and depressurization rate on the lipase activity by a full 23 experimental design.RESULTS: For both lyophilized and immobilized lipases from all sources evaluated, enhancement in residual activities after incubation in pressurized propane was observed, under several experimental conditions. The highest increment (427%) occurred with the lyophilized enzyme from P. simplicissimum was maintained pressurized at 30 bar for 1 h and then depressurized at the fastest rate (20 bar min−1).CONCLUSION: The enzyme activity changes significantly depending on the enzyme source, its presentation form, and the experimental pre‐treatment conditions investigated, such as exposure time, depressurization rate and pressure. The results obtained here can contribute as a basis for the selection of appropriate operational conditions, so that these catalysts can be applied in biotransformation reactions. Copyright © 2010 Society of Chemical Industry
Prey capture in frogs: alternative strategies, biomechanical trade‐offs, and hierarchical decision making
Authors: Monroy Jenna A. , Nishikawa Kiisa 
Published: March 2010

AbstractFrogs exhibit flexible repertoires of prey‐capture behavior, which depend primarily on visual analysis of prey attributes. We review three examples of how visual cues are used to modulate prey‐capture strategies. (1) Dyscophus guineti modulates tongue aiming in response to prey location. These frogs turn only their heads to apprehend prey located at azimuths <40°. At azimuths >40°, the frogs switch from this strategy to one in which both head and tongue are aimed toward prey. (2) Rana pipiens modulates its feeding behavior in response to prey size, using tongue prehension for capturing small prey but switching to jaw prehension to capture large prey. (3) In Cyclorana novaehollandiae, visual processing of prey attributes involves hierarchical decision making. These frogs first assess prey size. For large prey, they ignore velocity but not shape. For small prey, they ignore shape but not velocity. Alternative prey‐capture strategies are associated with biomechanical trade‐offs that result from the interaction between the feeding apparatus and varying attributes of prey. Alternative strategies likely exist because biomechanical constraints prevent any one strategy from being effective over a range of prey attributes. Taken together, these studies emphasize the requirement that predators must somehow tune prey‐capture kinematics simultaneously to multiple attributes of prey. In frogs, the choice among alternative prey‐capture strategies involves a hierarchical decision‐making process. Hierarchical decision making is expected to be widespread among animals. However, no previous studies were found except for humans, who frequently use this type of approach to make complex decisions. J. Exp. Zool. 315:61–71, 2011. © 2010 Wiley‐Liss, Inc.
Direct analysis of lipids in mouse brain using electrospray droplet impact/SIMS
Authors: Asakawa Daiki , Yoshimura Kentaro , Takeda Sen , Hiraoka Kenzo 
Published: March 2010

AbstractElectrospray droplet impact (EDI)/secondary ion mass spectrometry (SIMS) is a new desorption/ionization technique for mass spectrometry in which highly charged water clusters produced from the atmospheric‐pressure electrospray are accelerated in vacuum by 10 kV and impact the sample deposited on the metal substrate. EDI/SIMS was shown to enhance intact molecular ion formation dramatically compared to conventional SIMS. EDI/SIMS has been successfully applied to the analysis of mouse brain without any sample preparation. Five types of lipids, i.e. phosphatidylcholine (PC), phosphatidylserine, phosphatidylinositol (PI), galactocerebroside (GC) and sulfatide (ST), were readily detected from mouse brain section. In addition, by EDI/SIMS, six different regions of the mouse brain (cerebral cortex, corpus callosum, striatum, medulla oblongata, cerebellar cortex and cerebellar medulla) were examined. While GCs and STs were found to be rich in white matter, PIs were rich in gray matter. Copyright © 2010 John Wiley & Sons, Ltd.
Hydride transfer reactions via ion–neutral complex: fragmentation of protonated N‐benzylpiperidines and protonated N‐benzylpiperazines in mass spectrometry
Authors: Chai Yunfeng , Jiang Kezhi , Pan Yuanjiang 
Published: March 2010

AbstractAn ion‐neutral complex (INC)‐mediated hydride transfer reaction was observed in the fragmentation of protonated N‐benzylpiperidines and protonated N‐benzylpiperazines in electrospray ionization mass spectrometry. Upon protonation at the nitrogen atom, these compounds initially dissociated to an INC consisting of [RC6H4CH2]+ (R = substituent) and piperidine or piperazine. Although this INC was unstable, it did exist and was supported by both experiments and density functional theory (DFT) calculations. In the subsequent fragmentation, hydride transfer from the neutral partner to the cation species competed with the direct separation. The distribution of the two corresponding product ions was found to depend on the stabilization energy of this INC, and it was also approved by the study of substituent effects. For monosubstituted N‐benzylpiperidines, strong electron‐donating substituents favored the formation of [RC6H4CH2]+, whereas strong electron‐withdrawing substituents favored the competing hydride transfer reaction leading to a loss of toluene. The logarithmic values of the abundance ratios of the two ions were well correlated with the nature of the substituents, or rather, the stabilization energy of this INC. Copyright © 2010 John Wiley & Sons, Ltd.
Molecular dynamics simulations of MALDI: laser fluence and pulse width dependence of plume characteristics and consequences for matrix and analyte ionization
Authors: Knochenmuss Richard , Zhigilei Leonid V. 
Published: March 2010

AbstractMolecular dynamics simulations of matrix‐assisted laser desorption/ionization were carried out to investigate laser pulse width and fluence effects on primary and secondary ionization process. At the same fluence, short (35 or 350 ps) pulses lead to much higher initial pressures and ion concentrations than longer ones (3 ns), but these differences do not persist because the system relaxes toward local thermal equilibrium on a nanosecond timescale. Higher fluences accentuate the initial disparities, but downstream differences are not substantial. Axial velocities of ions and neutrals are found to span a wide range, and be fluence dependent. Total ion yield is only weakly dependent on pulse width, and consistent with experimental estimates. Secondary reactions of matrix cations with analyte neutrals are efficient even though analyte ions are ablated in clusters of matrix. Copyright © 2010 John Wiley & Sons, Ltd.
Gas phase isomeric differentiation of oleanolic and ursolic acids associated with heptakis‐(2,6‐di‐O‐methyl)‐β‐cyclodextrin by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry
Authors: Yu Zhan , Cui Meng , Yan Cunyu , Song Fengrui , Liu Zhiqiang , Liu Shuying , Zhang Hongxing 
Published: March 2010

AbstractOleanolic acid (OA) and ursolic acid (UA) are isomeric triterpenoid compounds with similar pharmaceutical properties. Usually, modern chromatographic and electrophoretic methods are widely utilized to differentiate these two compounds. Compared with mass spectrometric (MS) methods, these modern separation methods are both time‐ and sample‐consuming. Herein, we present a new method for structural differentiation of OA and UA by Fourier transform ion cyclotron resonance mass spectrometry (FT‐ICR MS) with the association of heptakis‐(2,6‐di‐O‐methyl)‐β‐cyclodextrin (DM‐β‐CD). Exact MS and tandem MS (MS/MS) data showed that there is no perceptible difference between OA and UA, as well as their β‐cyclodextrin and γ‐cyclodextrin complexes. However, there is a remarkable difference in MS/MS spectra of DM‐β‐CD complexes of OA and UA. The peak corresponding to the neutral loss of a formic acid and a water molecule could only be observed in the MS/MS spectrum of the complex of DM‐β‐CD : OA. Molecular modeling calculations were also employed to further investigate the structural differences of DM‐β‐CD : OA and DM‐β‐CD : UA complexes. Therefore, by employing DM‐β‐CD as a reference reagent, OA and UA could be differentiated with purely MS method. Copyright © 2010 John Wiley & Sons, Ltd.
Mass spectrometry of fluorocarbon‐labeled glycosphingolipids
Authors: Li Yunsen , Arigi Emma , Eichert Heather , Levery Steven B. 
Published: March 2010

AbstractA method for generation of novel fluorocarbon derivatives of glycosphingolipids (GSLs) with high affinity for fluorocarbon phases has been developed, and their potential applications to mass spectrometry (MS)‐based methodologies for glycosphingolipidomics have been investigated. Sphingolipid ceramide N‐deacylase (SCDase) is used to remove the fatty acid from the ceramide moiety, after which a fluorocarbon‐rich substituent (F‐Tag) is incorporated at the free amine of the sphingoid. In initial trials, a neutral GSL, globotriaosylceramide (Gb3Cer), three purified bovine brain gangliosides, and four fungal glycosylinositol phosphorylceramides (GIPCs) were de‐N‐acylated, derivatized by prototype F‐Tags, and recovered by solid phase extraction on fluorocarbon‐derivatized silica (F‐SPE). The efficacy of SCDase treatment of GIPCs was here demonstrated for the first time. Compatibility with subsequent per‐N,O‐methylation was established for the F‐tagged Gb3 Cer and purified gangliosides, and extensive mass spectra (MS1 and MS2) consistent with all of the expected products were acquired. The potential use of F‐tagged derivatives for a comprehensive MS based profiling application was then demonstrated on a crude ganglioside mixture extracted from bovine brain. Finally, a simple trial in microarray format demonstrated fixation of F‐tagged GM1 ganglioside to a fluorous glass surface, with the glycan intact and available for interaction with a fluorescent derivative of cholera toxin B chain. The methods described thus provide a new avenue for rapid GSL recovery or cleanup, potentially compatible with a variety of platforms for mass spectrometric profiling and structure analysis, as well as parallel analysis of functional interactions. Copyright © 2010 John Wiley & Sons, Ltd.
A study of the cesium cation bonding to carboxylate anions by the kinetic method and quantum chemical calculations
Authors: Mayeux Charly , Gal Jean‐François , Charles Laurence , Massi Lionel , Maria Pierre‐Charles , Tammiku‐Taul Jaana , Lohu Ene‐Liis , Burk Peeter 
Published: March 2010

AbstractCollision‐induced dissociation (CID) of the Cs+ heterodimer adducts of the nitrate anion (NO3−) and a variety of substituted benzoates (XBenz−) [(XBenz−)(Cs+)(NO3−)]− produces essentially nitrate and benzoate ions. A plot of the natural logarithm of their intensity ratio, ln[I (NO3−)/I(XBenz−)], versus the calculated cesium cation affinity (DFT B3LYP) of the substituted benzoate ions (equivalent to the enthalpy of heterolytic dissociation of the salt) is reasonably linear. This suggests that the kinetic method can be used as a source of data on the intrinsic interaction between the anionic and the cationic moieties in a salt. Copyright © 2010 John Wiley & Sons, Ltd.
Osteoblastic differentiation of human and equine adult bone marrow‐derived mesenchymal stem cells when BMP‐2 or BMP‐7 homodimer genetic modification is compared to BMP‐2/7 heterodimer genetic modification in the presence and absence of dexamethasone
Authors: Carpenter Ryan S. , Goodrich Laurie R. , Frisbie David D. , Kisiday John D. , Carbone Beth , McIlwraith C. Wayne , Centeno Christopher J. , Hidaka Chisa 
Published: March 2010

AbstractBone marrow‐derived mesenchymal stem cells (BMDMSCs) have been targeted for use in enhancement of bone healing; and their osteogenic potential may be further augmented by genes encoding bone morphogenetic proteins (BMP's). The purpose of this study was to compare the effect of genetic modification of human and equine BMDMSCs with BMP‐2 or ‐7 or BMP‐2 and ‐7 on their osteoblastogenic differentiation in the presence or absence of dexamethasone. The BMDMSCs were harvested from the iliac crest of three human donors and tuber coxae of three equine donors. Monolayer cells were genetically modified using adenovirus vectors encoding BMP‐2, ‐7 or both and cultured in the presence or absence of dexamethasone. Expression of BMPs was confirmed by enzyme linked immunosorbent assay (ELISA). To evaluate osteoblastic differentiation, cellular morphology was assessed every other day and expression and secretion of alkaline phosphatase (ALP), as well as expression levels of osteonectin (OSTN), osteocalcin (OCN), and runt‐related transcription factor‐2 (Runx2) were measured for up to 14 days. Human and equine BMDMSCs showed a capacity for osteogenic differentiation regardless of genetic modification or dexamethasone supplementation. Dexamethasone supplementation was more important for osteoblastogenic differentiation of equine BMDMSCs than human BMDMSCs. Genetic modification of BMDMSCs increased ALP secretion with AdBMP‐2 homodimer having the greatest effect in both human and equine cells compared to AdBMP 7 or AdBMP 2/7. BMP protein elution rates reached their maximal concentration between day 4 and 8 and remained relatively stable thereafter, suggesting that genetically modified BMDMSCs could be useful for cell‐based delivery of BMPs to a site of bone formation. Published by Wiley Periodicals, Inc. J Orthop Res 28:1330–1337, 2010
Analysis of frictional behavior and changes in morphology resulting from cartilage articulation with porous polyurethane foams
Authors: Gleghorn Jason P. , Doty Stephen B. , Warren Russell F. , Wright Timothy M. , Maher Suzanne A. , Bonassar Lawrence J. 
Published: March 2010

AbstractPorous polyurethane foams (PUR) have been extensively evaluated as meniscal replacement materials and show great promise enabling infiltration of cells and fibrocartilage formation in vivo. Similar to most materials, PUR demonstrates progressive degeneration of opposing cartilage; however, the damage mechanism is impossible to determine because no information exists on the frictional properties of PUR–cartilage interfaces. The goals of this study were to characterize the frictional behavior of a cartilage–PUR interface across a range of articulating conditions and assess the resulting morphological changes to the cartilage surface following articulation. Articular cartilage was oscillated against PUR or stainless steel using phosphate‐buffered saline (PBS) and synovial fluid as lubricants. Following friction testing, cartilage and PUR samples were analyzed with environmental scanning electron microscopy and histological staining to determine changes in tissue morphology. Stribeck‐surface analysis demonstrated distinct lubrication modes; however, boundary mode lubrication was dominant in cartilage–PUR interfaces and the low‐friction pressure‐borne lubrication mechanism present in native joints was absent. Microscopy noted obvious wear, with disruption of the collagen architecture and concomitant proteoglycan loss in cartilage articulated against PUR. These data collectively point to the importance of frictional properties as design parameters for implants and materials for soft tissue replacement. Published by Wiley Periodicals, Inc. J Orthop Res 28:1292–1299, 2010