bluuebunny/crossref_metadata_2025_split huggingface dataset:
Diagnosis and management of maternal thrombocytopenia in pregnancy
Authors: Myers Bethan 
Published: May 2012

SummaryThrombocytopenia is a common finding in pregnancy, occurring in approximately 7–10% of pregnancies. It may be a diagnostic and management problem, and has many causes, some of which are specific to pregnancy. Although most cases of thrombocytopenia in pregnancy are mild, and have no adverse outcome for either mother or baby, occasionally a low platelet count may be part of a more complex disorder with significant morbidity and may be life‐threatening. Overall, about 75% of cases are due to gestational thrombocytopenia, 15–20% secondary to hypertensive disorders; 3–4% due to an immune process, and the remaining 1–2% made up of rare constitutional thrombocytopenias, infections and malignancies. In this review, a diagnostic approach to investigating thrombocytopenia in pregnancy is presented, together with antenatal, anaesthetic and peri‐natal management issues for mother and baby, followed by a detailed discussion on the specific causes of thrombocytopenia and the management options in each case.
Calcium alginate‐resistant starch mixed gel improved the survival of Bifidobacterium animalis subsp. lactis Bb12 and Lactobacillus rhamnosus LBRE‐LSAS in yogurt and simulated gastrointestinal conditions
Authors: Ziar Hasnia , Gérard Philippe , Riazi Ali 
Published: May 2012

SummaryThe aim of this study was to investigate the protective effect of microencapsulation in calcium alginate‐resistant starch mixed gel of a new human isolated strain of Lactobacillus rhamnosus LBRE‐LSAS compared with the probiotic strain of Bifidobacterium animalis subsp. lactis Bb12. Influence of microencapsulation was tested under deleterious digestive environment, when challenged to salivary α‐amylase, to simulated gastric fluid and to simulated intestinal fluid. Bacterial survival, post‐acidifying activity and exopolysaccharides (EPS) content in stored mix yogurt were assessed. Integrity of the beads was acceptable under α‐amylase levels largely higher than those found in human saliva. Under simulated gastrointestinal model, viable cell counts of encapsulated cells were significantly higher than those observed with free cells and remained at the recommended levels. Additionally, microencapsulation allowed an improved viability of bacteria and generated higher EPS amounts in mix yogurt stored at 4 °C. Our results indicate that calcium alginate‐resistant starch beads extend survival under digestive conditions and in yogurt and could be used as an efficient delivery system for probiotics.
Facilitation of tiger moths by outbreaking tussock moths that share the same host plants
Authors: Karban Richard , Grof‐Tisza Patrick , Holyoak Marcel 
Published: May 2012

Summary1. Ecologists have argued about the commonness and strength of interspecific competition between insect herbivores, but facilitation between herbivores has received much less consideration. We previously found that when two species of folivorous caterpillars co‐occurred on a shared host plant, feeding by early season tiger moth caterpillars reduced the growth and reproduction of later season tussock caterpillars. However, densities of tussock caterpillars in summer were positively correlated with densities of tiger moth caterpillars the following spring.2. In this study, we experimentally manipulated numbers of feeding tussock caterpillars and found that they facilitated tiger moth caterpillars.3. The depth of the litter layer beneath host lupine bushes was positively correlated with the number of tussock caterpillars feeding on each bush. Experimental additions of litter beneath lupine canopies during summer resulted in increased numbers of tiger moth caterpillars in the following spring, indicating a causal role of litter. Litter potentially provides food, habitat and protection from desiccation and predation. We failed to find evidence that tussock caterpillars facilitated tiger moth caterpillars by mechanisms independent of litter.4. Our study demonstrates that facilitation may operate between insect herbivores, across life‐stages through indirect interactions that are non‐trophic. Facilitation operated by a novel mechanism, the accumulation of litter which was a by‐product of feeding by one species was valuable to a second species. Facilitation persisted in time and space far beyond the creation of litter by tussock caterpillars which should be considered important ecosystem engineers from the point of view of tiger moths. Facilitations that involve habitat modification may generally connect species that do not interact directly or trophically, and have not previously been considered to affect one another.
The putative protein methyltransferase LAE1 controls cellulase gene expression in Trichoderma reesei
Authors: Seiboth Bernhard , Karimi Razieh Aghcheh , Phatale Pallavi A. , Linke Rita , Hartl Lukas , Sauer Dominik G. , Smith Kristina M. , Baker Scott E. , Freitag Michael , Kubicek Christian P. 
Published: May 2012

SummaryTrichoderma reesei is an industrial producer of enzymes that degrade lignocellulosic polysaccharides to soluble monomers, which can be fermented to biofuels. Here we show that the expression of genes for lignocellulose degradation are controlled by the orthologous T. reesei protein methyltransferase LAE1. In a lae1 deletion mutant we observed a complete loss of expression of all seven cellulases, auxiliary factors for cellulose degradation, β‐glucosidases and xylanases were no longer expressed. Conversely, enhanced expression of lae1 resulted in significantly increased cellulase gene transcription. Lae1‐modulated cellulase gene expression was dependent on the function of the general cellulase regulator XYR1, but also xyr1 expression was LAE1‐dependent. LAE1 was also essential for conidiation of T. reesei. Chromatin immunoprecipitation followed by high‐throughput sequencing (‘ChIP‐seq’) showed that lae1 expression was not obviously correlated with H3K4 di‐ or trimethylation (indicative of active transcription) or H3K9 trimethylation (typical for heterochromatin regions) in CAZyme coding regions, suggesting that LAE1 does not affect CAZyme gene expression by directly modulating H3K4 or H3K9 methylation. Our data demonstrate that the putative protein methyltransferase LAE1 is essential for cellulase gene expression in T. reesei through mechanisms that remain to be identified.
Efficient control of Plasmodium yoelii infection in BALB/c and C57BL/6 mice with pre‐existing Strongyloides ratti infection
Authors: KOLBAUM J. , ESCHBACH M.‐L. , STEEG C. , JACOBS T. , FLEISCHER B. , BRELOER M. 
Published: May 2012

SummaryAbout 225 million malaria cases have been reported worldwide in 2009, and one‐third of the world’s population is infected with parasitic helminths. As helminths and Plasmodium are co‐endemic, concurrent infections frequently occur. Helminths have been shown to modulate the host’s immune response; therefore, pre‐existing helminth infections may interfere with the efficient immune response to Plasmodium. To study the interaction between helminths and Plasmodium, we established a murine model of co‐infection using the gastrointestinal nematode Strongyloides ratti and Plasmodium yoelii. We show that a pre‐existing Strongyloides infection slightly enhanced peak parasitemia and weight loss in P. yoelii‐infected BALB/c mice, while disease progression was not altered in co‐infected C57BL/6 mice. The Plasmodium‐induced IFN‐γ production and final clearance of Plasmodium infection were not affected by S. ratti co‐infection in both C57BL/6 and BALB/c mice. Interestingly, the T helper cell (Th) 2 response induced by S. ratti was significantly suppressed upon P. yoelii co‐infection. This suppressed Th2 response, however, was still sufficient to allow expulsion of S. ratti parasitic adults. Taken together, we provide evidence that simultaneous presence of helminth and protist parasites does not interfere with efficient host defence in our co‐infection model although changes in Th responses were observed.
Phytochrome B increases drought tolerance by enhancing ABA sensitivity in Arabidopsis thaliana
Authors: GONZÁLEZ CARINA VERÓNICA , IBARRA SILVIA ELIZABETH , PICCOLI PATRICIA NOEMÍ , BOTTO JAVIER FRANCISCO , BOCCALANDRO HERNÁN ESTEBAN 
Published: May 2012

ABSTRACTPhytochrome B (phyB) can adjust morphological and physiological responses according to changes in the red : far‐red (R:FR) ratio. phyB‐driven acclimation of plants to open environments (high R:FR ratio) increases carbon gain at the expense of increased water loss. This behaviour alleviates stressful conditions generated by an excess of light, but increases the chances of desiccation. Here we evaluated how phyB modulates this drought‐tolerance response by comparing wild‐type Arabidopsis thaliana adult plants to the null phyB in response to water shortage. phyB wilted before the wild type, and this was due to phyB maintaining open stomata under a reduction in soil water availability. Although phyB presented enhanced ABA levels under well‐watered conditions, this mutant was less sensitive than the wild type in diminishing stomatal conductance in response to exogenous ABA application. Reduced sensitivity to ABA in phyB correlated with a lower expression of ABCG22, which encodes a putative ABA influx transporter, and PYL5, which encodes a soluble ABA receptor. Furthermore, the expression of RAB18 and RD29A, both typical ABA‐induced genes, was lower in phyB than the wild type after ABA treatment. We propose that phyB contributes to the acclimation of plants to open environments by enhancing ABA sensitivity when soil water becomes limiting.
Whole exome sequencing identifies a novel mutation in the transglutaminase 6 gene for spinocerebellar ataxia in a Chinese family
Authors: Li M , Pang SYY , Song Y , Kung MHW , Ho S‐L , Sham P‐C 
Published: May 2012

Li M, Pang SYY, Song Y, Kung MHW, Ho S‐L, Sham P‐C. Whole exome sequencing identifies a novel mutation in the transglutaminase 6 gene for spinocerebellar ataxia in a Chinese family.Autosomal dominant spinocerebellar ataxias (SCA) constitute a heterogeneous group of inherited disorders. The transglutaminase 6 (TGM6) gene was recently suggested as a SCA causative gene in Chinese SCA families. In this study, two affected members of a three‐generation Chinese family with SCA characterized by progressive cerebellar ataxia and lower limb pyramidal signs were subjected to whole exome sequencing. Through bioinformatics analysis of the sequence variants in these two individuals, we identified a novel mutation in the TGM6 gene (c.1528G>C) which showed perfect co‐segregation with disease phenotype in all nine members of this family. This finding confirms that mutations in TGM6 gene represent an important cause of SCA in Chinese. This study also shows that whole exome sequencing of a small number of affected individuals, leveraged on bioinformatics analysis, can be an efficient strategy for identifying causative mutations in rare Mendelian disorders.
Pneumocystis jirovecii pneumonia 13 years post renal transplant following a recurrent cytomegalovirus infection
Authors: Muhammad Iqbal A.H. , Lim S.K. , Ng K.P. , Tan L.P. , Chong Y.B. , Keng T.C. 
Published: May 2012

Abstract
Pneumocystis jirovecii (formerly Pneumocystis carinii) pneumonia (PCP) is a rare but serious infection that usually occurs within a year after solid organ transplantation. PCP may occur after 1 year post transplantation, but the rate is reported to be very low. Studies have shown an association between cytomegalovirus (CMV) infection in solid organ transplant patients and an increased risk of opportunistic infection. This increased risk is thought to be a result of the immunomodulatory effects of the CMV infection. We present a case of PCP infection occurring 13 years after a renal transplantation. This occurred following a recurrent CMV infection while the patient was on low‐dose immunosuppressants.
Intermittent ischemia enhances the uptake of indocyanine green to livers subject to ischemia and reperfusion
Authors: Steenks Mathilde , Peters Jeroen , Rademacher Willem , Nieuwenhuijs Vincent B , Padbury Robert T A , Barritt Greg J 
Published: May 2012

AbstractBackground and AimIntermittent ischemia is known to promote post perfusion bile flow, and hence recovery of liver function following ischemia reperfusion of the liver. However, the mechanisms involved are not well understood. The aim of this study was to identify the step(s) in the bile acid transport pathway altered by intermittent ischemia.MethodsArat model of segmental hepatic ischemia in which the bilateral median and left lateral lobes were made ischemic by clamping the blood vessels was used. Indocyanine green (ICG), infrared spectroscopy, and compartmental kinetic analysis, were used to indirectly monitor the movement of bile acids across hepatocytes in situ. Rates of bile flow were measured gravimetrically.ResultsIn control livers (not subjected to ischemia), the movement of ICG from the blood to bile fluid could be described by a three compartment model comprising the blood, a rapidly‐exchangeable compartment, and the hepatocyte cytoplasmic space. In livers subjected to continuous clamping, the rates of ICG uptake to the liver, and outflow from the liver, were greatly reduced compared with those in control livers. Intermittent clamping (three episodes of 15 min clamping) compared with continuous clamping substantially increased the rate of ICG uptake from the blood but had less effect on the rate of ICG outflow from hepatocytes.ConclusionsIt is concluded that intermittent ischemia promotes post reperfusion bile flow in the early phase of ischemia reperfusion injury principally by enhancing the movement of bile acids from the blood to hepatocytes.
Lower gastrointestinal symptoms are prevalent among individuals colonoscoped within the Bowel Cancer Screening Programme
Authors: Rajasekhar P. T. , Ritchie M. , Rutter M. D. , Clifford G. , Waddup G. , Dempsey N. , Rubin G. P. , Rees C. J. 
Published: May 2012

AbstractAim  The National Health Service Bowel Cancer Screening Programme (BCSP) aims to detect earlier stage cancer in asymptomatic individuals. Early experience suggested that many participants had lower gastrointestinal symptoms before screening. The study evaluated the prevalence of lower gastrointestinal symptoms and consultation behaviour among individuals undergoing colonoscopy at the South of Tyne BCSP Centre.Method  Data were collected on all undergoing clinic assessment and colonoscopy. Symptoms were categorized as altered bowel habit (ABH), rectal bleeding (RB), abdominal pain (AP) and unexplained weight loss (UWL).Results  Symptoms were present in 65.1% (492/756) of subjects, 64.4% (431/669) of those with a non‐cancer diagnosis and 70.1% (61/87) of those with cancer. Among those with a non‐cancer diagnosis, symptoms were ABH in 52% (224/431), RB in 81.4% (351/431), AP in 15.3% (66/431) and UWL in 3.0% (13/431). In those with cancer symptoms they were ABH in 33.3% (29/87), RB in 55.2% (48/87) and AP in 11.5% (10/87). There was no significant difference in the prevalence of symptoms in those with a cancer or non‐cancer diagnosis. A total of 34.2% (157/459) of individuals with symptoms had consulted their general practitioner, 28.1% (16/57) of those with cancer and 35.1% (141/402) without.Conclusion  A large proportion of individuals colonoscoped in the BCSP reported symptoms predating screening. Their prevalence did not differ significantly between cancer and non‐cancer diagnoses. The majority had not consulted their general practitioner. Health promotion regarding the importance of lower gastrointestinal symptoms and a risk assessment tool to help select those needing urgent specialist assessment are required.
Geospatial Information Integration for Authoritative and Crowd Sourced Road Vector Data
Authors: Du Heshan , Anand Suchith , Alechina Natasha , Morley Jeremy , Hart Glen , Leibovici Didier , Jackson Mike , Ware Mark 
Published: May 2012

AbstractThis article describes results from a research project undertaken to explore the technical issues associated with integrating unstructured crowd sourced data with authoritative national mapping data. The ultimate objective is to develop methodologies to ensure the feature enrichment of authoritative data, using crowd sourced data. Users increasingly find that they wish to use data from both kinds of geographic data sources. Different techniques and methodologies can be developed to solve this problem. In our previous research, a position map matching algorithm was developed for integrating authoritative and crowd sourced road vector data, and showed promising results (Anand et al. 2010). However, especially when integrating different forms of data at the feature level, these techniques are often time consuming and are more computationally intensive than other techniques available. To tackle these problems, this project aims at developing a methodology for automated conflict resolution, linking and merging of geographical information from disparate authoritative and crowd‐sourced data sources. This article describes research undertaken by the authors on the design, implementation, and evaluation of algorithms and procedures for producing a coherent ontology from disparate geospatial data sources. To integrate road vector data from disparate sources, the method presented in this article first converts input data sets to ontologies, and then merges these ontologies into a new ontology. This new ontology is then checked and modified to ensure that it is consistent. The developed methodology can deal with topological and geometry inconsistency and provide more flexibility for geospatial information merging.
The Annotation Process in OpenStreetMap
Authors: Mooney Peter , Corcoran Padraig 
Published: May 2012

AbstractIn this article we describe the analysis of 25,000 objects from the OpenStreetMap (OSM) databases of Ireland, United Kingdom, Germany, and Austria. The objects are selected as exhibiting the characteristics of “heavily edited” objects. We consider “heavily edited” objects as having 15 or more versions over the object's lifetime. Our results indicate that there are some serious issues arising from the way contributors tag or annotate objects in OSM. Values assigned to the “name” and “highway” attributes are often subject to frequent and unexpected change. However, this “tag flip‐flopping” is not found to be strongly correlated with increasing numbers of contributors. We also show problems with usage of the OSM ontology/controlled vocabularly. The majority of errors occurring were caused by contributors choosing values from the ontology “by hand” and spelling these values incorrectly. These issues could have a potentially detrimental effect on the quality of OSM data while at the same time damaging the perception of OSM in the GIS community. The current state of tagging and annotation in OSM is not perfect. We feel that the problems identified are a combination of the flexibility of the tagging process in OSM and the lack of a strict mechanism for checking adherence to the OSM ontology for specific core attributes. More studies related to comparing the names of features in OSM to recognized ground‐truth datasets are required.
The Global Production of Transportation Public–Private Partnerships
Authors: Siemiatycki Matti 
Published: May 2012

AbstractAround the world, public–private partnerships have become increasingly popular to deliver large‐scale transportation infrastructure projects such as roads, bridges, railways, subways, seaports and airports. The aim of this article is to provide a framework to understand the global geography of projects built through this market‐driven procurement model, which have been predominantly concentrated in a small number of developed countries and emerging markets. As is shown, within many countries, a governance and regulatory environment has been established that supports public–private partnerships over other alternative procurement approaches. Nevertheless, the production of public–private partnerships worldwide has been dominated by a relatively small number of highly globalized construction contractors, engineering firms, financiers, accountancies and consultants from developed countries, who have focused their activities in a narrow set of regions. The article concludes by reflecting on the implications of the high level of industry concentration, and emerging trends showing greater involvement from firms from developing countries.ResuméÀ travers le monde, les partenariats public‐privé sont de plus en plus utilisés pour les projets d’infrastructure de transports de grande envergure, tels que routes, ponts, voies ferrées, métros, ports et aéroports. Cet article définit un cadre permettant de comprendre la géographie planétaire des projets bâtis selon ce modèle orienté par le marché, projets concentrés dans un petit nombre de pays développés et émergents. Les régimes de gouvernance et la réglementation mis en place dans de nombreux pays favorisent les partenariats public‐privé au détriment d’autres approches de réalisation de projets. Néanmoins, la production de ces partenariats dans le monde a été dominée par un nombre relativement réduit d’entreprises de bâtiment et d’ingénierie, de bailleurs de fonds, de cabinets de comptabilité et de conseils, acteurs installés dans des pays développés et dont les activités internationalisées s’exercent sur un ensemble restreint de régions. La conclusion aborde les conséquences d’une forte concentration industrielle et l’apparition d’une implication croissante des entreprises des pays en développement.
Comparison of Ventricular Radiofrequency Lesions in Sheep Using Standard Irrigated Tip Catheter versus Catheter Ablation Enabling Direct Visualization
Authors: SACHER FREDERIC , DERVAL NICOLAS , JADIDI AMIR , SCHERR DANIEL , HOCINI MELEZE , HAISSAGUERRE MICHEL , SANTOS PIERRE DOS , JAIS PIERRE 
Published: May 2012

Comparison of Ventricular Radiofrequency Lesions in Sheep. Introduction: In vivo assessment of RF ablation lesions is limited. Improved feedback could affect procedural outcome. A novel catheter, IRIS™ Cardiac Ablation Catheter (IRIS), enabling direct tissue visualization during ablation, was compared to a 3.5 mm open‐irrigated tip ThermoCool™ Catheter (THERM) for endocardial ventricular RF ablation in sheep.Methods: Sixteen anesthetized sheep (6 ± 1 years old, 60 ± 10 kg) underwent ventricular RF applications with either the THERM (Biosense Webster) or IRIS (Voyage Medical) ablation catheter. In the THERM group, RF was delivered (30 W, 60 seconds) when electrode contact was achieved as assessed by recording high‐amplitude electrogram, tactile feedback, and x‐ray. In the IRIS group, direct visualization was used to confirm tissue contact and to guide energy delivery (10–25 W for 60 seconds) depending on visual feedback during lesion formation.Results: A total of 160 RF applications were delivered (80 with THERM; 80 with IRIS). Average power delivery was significantly higher in the THERM group than in the IRIS group (30 ± 2 W [25–30 W] for 57 ± 14 seconds vs 21 ± 4 W [10–25 W] for 57 ± 27 seconds; P<0.001). At necropsy, 62/80 (78%) lesions created with THERM were identified versus 79/80 (99%) with IRIS (P<0.001). The lesion dimensions were not significantly different between THERM and IRIS.Conclusion: Despite best efforts using standard clinical assessments of catheter contact, 22% of RF applications in the ventricles using a standard open‐irrigated catheter could not be identified on necropsy. In vivo assessment of catheter contact by direct visualization of the tissue undergoing RF ablation with the IRIS™ catheter was more reliable by allowing creation of 99% prescribed target lesions without significant complications. (J Cardiovasc Electrophysiol, Vol. 23, pp. 869‐873, August 2012)