bluuebunny/crossref_metadata_2025_split huggingface dataset:
First detection of Ichthyophonus sp. in invasive wild pink salmon (Oncorhynchus gorbuscha) from the North Atlantic Ocean
Authors: Erkinharju Toni , Hansen Haakon , Garseth Åse Helen 
Published: June 2024

AbstractPacific pink salmon (Oncorhynchus gorbuscha) were deliberately introduced to rivers surrounding the White Sea and has spread to Norway and several other countries surrounding the North Atlantic Ocean. In August 2021, a female pink salmon displaying pale gills and abnormal behaviour was captured in River Lakselva in Northern Norway and later submitted to the Norwegian Veterinary Institute (NVI) for post‐mortem examination. Histological examination of organ samples revealed structures indicative of systemic ichthyophoniasis, caused by Ichthyophonus sp. The parasites appeared to be especially abundant in the heart and skeletal musculature, and local tissue responses were assessed to be absent or very mild. Sequences of the ribosomal 18S rRNA and the mitochondrial cytochrome oxidase 1 (CO1) genes confirmed the diagnosis and identified the pathogen as Ichthyophonus sp. The CO1 sequence further established that the isolate from pink salmon was most similar to sequences of Ichthyophonus sp. from Atlantic salmon, Salmo salar, from the Atlantic Ocean on the east coast of the US and from Atlantic herring, Clupea harengus, from Iceland. We here report the first detection of Ichthyophonus sp. in pink salmon in the North Atlantic Ocean.
Overexpression of tonoplast Ca2+‐ATPase in guard cells synergistically enhances stomatal opening and drought tolerance
Authors: Su Jinghan , He Bingqing , Li Peiyuan , Yu Baiyang , Cen Qiwen , Xia Lingfeng , Jing Yi , Wu Feibo , Karnik Rucha , Xue Dawei , Blatt Michael R. , Wang Yizhou 
Published: June 2024

ABSTRACTStomata play a crucial role in plants by controlling water status and responding to drought stress. However, simultaneously improving stomatal opening and drought tolerance has proven to be a significant challenge. To address this issue, we employed the OnGuard quantitative model, which accurately represents the mechanics and coordination of ion transporters in guard cells. With the guidance of OnGuard, we successfully engineered plants that overexpressed the main tonoplast Ca2+‐ATPase gene, ACA11, which promotes stomatal opening and enhances plant growth. Surprisingly, these transgenic plants also exhibited improved drought tolerance due to reduced water loss through their stomata. Again, OnGuard assisted us in understanding the mechanism behind the unexpected stomatal behaviors observed in the ACA11 overexpressing plants. Our study revealed that the overexpression of ACA11 facilitated the accumulation of Ca2+ in the vacuole, thereby influencing Ca2+ storage and leading to an enhanced Ca2+ elevation in response to abscisic acid. This regulatory cascade finely tunes stomatal responses, ultimately leading to enhanced drought tolerance. Our findings underscore the importance of tonoplast Ca2+‐ATPase in manipulating stomatal behavior and improving drought tolerance. Furthermore, these results highlight the diverse functions of tonoplast‐localized ACA11 in response to different conditions, emphasizing its potential for future applications in plant enhancement.
Rice stripe mosaic virus hijacks rice heading‐related gene to promote the overwintering of its insect vector
Authors: Chen Siping , Zhong Xinyi , Wang Zhiyi , Chen Biao , Huang Xiuqin , Xu Sipei , Yang Xin , Zhou Guohui , Zhang Tong 
Published: June 2024

ABSTRACTRice stripe mosaic virus (RSMV) is an emerging pathogen which significantly reduces rice yields in the southern region of China. It is transmitted by the leafhopper Recilia dorsalis, which overwinters in rice fields. Our field investigations revealed that RSMV infection causes delayed rice heading, resulting in a large number of green diseased plants remaining in winter rice fields. This creates a favorable environment for leafhoppers and viruses to overwinter, potentially contributing to the rapid spread and epidemic of the disease. Next, we explored the mechanism by which RSMV manipulates the developmental processes of the rice plant. A rice heading‐related E3 ubiquitin ligase, Heading date Associated Factor 1 (HAF1), was found to be hijacked by the RSMV‐encoded P6. The impairment of HAF1 function affects the ubiquitination and degradation of downstream proteins, HEADING DATE 1 and EARLY FLOWERING3, leading to a delay in rice heading. Our results provide new insights into the development regulation‐based molecular interactions between virus and plant, and highlights the importance of understanding virus‐vector‐plant tripartite interactions for effective disease management strategies.
The TaGW2‐TaSPL14 module regulates the trade‐off between tiller number and grain weight in wheat
Authors: Jian Chao , Pan Yuxue , Liu Shujuan , Guo Mengjiao , Huang Yilin , Cao Lina , Zhang Weijun , Yan Liuling , Zhang Xueyong , Hou Jian , Hao Chenyang , Li Tian 
Published: June 2024

ABSTRACTIDEAL PLANT ARCHITECTURE1 (IPA1) is a pivotal gene controlling plant architecture and grain yield. However, little is known about the effects of Triticum aestivum SQUAMOSA PROMOTER‐BINDING‐LIKE 14 (TaSPL14), an IPA1 ortholog in wheat, on balancing yield traits and its regulatory mechanism in wheat (T. aestivum L.). Here, we determined that the T. aestivum GRAIN WIDTH2 (TaGW2)‐TaSPL14 module influences the balance between tiller number and grain weight in wheat. Overexpression of TaSPL14 resulted in a reduced tiller number and increased grain weight, whereas its knockout had the opposite effect, indicating that TaSPL14 negatively regulates tillering while positively regulating grain weight. We further identified TaGW2 as a novel interacting protein of TaSPL14 and confirmed its ability to mediate the ubiquitination and degradation of TaSPL14. Based on our genetic evidence, TaGW2 acts as a positive regulator of tiller number, in addition to its known role as a negative regulator of grain weight, which is opposite to TaSPL14. Moreover, combinations of TaSPL14‐7A and TaGW2‐6A haplotypes exhibit significantly additive effects on tiller number and grain weight in wheat breeding. Our findings provide insight into how the TaGW2‐TaSPL14 module regulates the trade‐off between tiller number and grain weight and its potential application in improving wheat yield.
An Integrated Approach to Address Perinatal Mental Health Within an Obstetrics Practice
Authors: Felten Christina L. , Smith Kayla S. , Aylesworth Melissa B. 
Published: June 2024

AbstractOutpatient perinatal care providers (one certified nurse‐midwife, one nurse practitioner, and one physician assistant) at a high‐volume, suburban health system in southeastern Pennsylvania developed and implemented a care model to identify and care for patients at risk for perinatal and postpartum mental health conditions. The program, Women Adjusting to Various Emotional States (WAVES), was created to bring the most up‐to‐date, evidence‐based treatment recommendations to patients while addressing the increased demand placed on the health care system by pregnant and postpartum patients in need of psychiatric services. WAVES is a specialized program offered for anyone who is pregnant or up to one year postpartum who is struggling with mental health symptoms or concerns. Perinatal mood and anxiety disorders have become one of the most prevalent pregnancy ailments, yet mental health is not always addressed during routine prenatal care visits. Common obstacles to patients obtaining mental health care during pregnancy include lack of access, clinician gaps in knowledge, and stigma surrounding diagnoses. WAVES offers a method to empower perinatal providers with the education and tools to address this need. The model outlines how to appropriately assess, diagnose, manage, or refer patients for mental health services. Patient feedback has been overwhelmingly positive, and this novel care model shows great promise for the future of perinatal care. The development of integrated programs like WAVES may be a valuable resource to help combat the perinatal mental health epidemic.
GEOCHEMISTRY OF OILS AND GASES FROM THE VERKHNECHONSKOYE FIELD, EAST SIBERIAN BASIN: APPLICATION OF ANALYTICAL RESULTS TO RESERVOIR CHARACTERISATION
Authors: Oblasov N. V. , Goncharov I. V. , Eftor I. V. , van Graas G. W. , Veklich M. A. , Akhmadishin A. T. , Lokshin D. A. 
Published: June 2024

A geochemical study was carried out on oil and gas samples from the Verkhnechonskoye field, located on the Nepa‐Botuoba Anteclise in the central‐southern part of the Siberian Platform. The goal of the study was to distinguish between fluids derived from the V10‐13 and B12 reservoir units in the Vendian (Neoproterozoic) Katanga and Nepa Formations and to identify the producing reservoir using geochemical data. The results of analyses of 12 oil and 13 associated gas samples from the two reservoirs showed that all the fluids have similar geochemical properties including: low Pr/Ph ratios (0.78‐1.00); a predominance of C29 over C27 and C28 steranes; a predominance of odd‐numbered C21‐C25 n‐alkylbenzenes over their even‐numbered homologues; the presence of 12‐ and 13‐methylalkanes; and a high relative abundance of tricyclic terpanes (cheilantanes). All these properties are consistent with those of the properties of petroleum from other fields on the Siberian Platform. The molecular and stable carbon isotope compositions of the oils and gases suggest that they were derived from marine organic matter with a high algal input deposited under reducing conditions. To date, specific source rocks which generated the oil and gas present at fields on the Nepa‐Botuaoba Anteclise have not conclusively been identified, but potential candidates include the Upper Riphean Iremeken and Ayan Formations and more probably the Vendian Zherbinskaya, Seralakh, Vanavara and Nepa Formations.The second part of the study demonstrates the application to reservoir geochemistry of C3‐ and C4‐ alkylbenzene compounds together with more conventional biomarkers. Key parameters were selected using statistical processing and displayed in graphic profiles. These profiles allowed the oil and gas samples to be classified according to the reservoir from which they were derived based on their geochemical properties. Parameters based on C3‐ and C4‐ alkylbenzene compounds were most effective in discriminating between oils from the two reservoirs. In addition, a new parameter is proposed based on the contents of 1‐methyl‐3‐isopropylbenzene, 1‐methyl‐2‐isopropylbenzene and 1‐methyl‐2‐propylbenzene; this parameter correlates closely with the pristane/phytane ratio and can be used as an additional indicator of the level of oxicity in the source rock depositional environment.
Multiple resistance of Colletotrichum truncatum from soybean to QoI and MBC fungicides in Brazil
Authors: Rogério Flávia , de Castro Renata Rebellato Linhares , Massola Júnior Nelson Sidnei , Boufleur Thaís Regina , dos Santos Ricardo Feliciano 
Published: June 2024

AbstractColletotrichum truncatum, the predominant fungal species associated with soybean anthracnose, is responsible for significant losses in this crop. Chemical control via fungicide application is the most effective strategy for the control of soybean foliar diseases. However, the increasing incidence of anthracnose in some regions of Brazil indicates that current chemical control is not effective against anthracnose. In this study, we evaluated the fungicide sensitivity of C. truncatum genetic lineages to the fungicides azoxystrobin, thiophanate‐methyl, difenoconazole, and fludioxonil using isolates representing two important regions of soybean production in Brazil. We characterized the molecular resistance to the quinone‐outside inhibitors (QoI), methyl benzimidazole carbamates (MBC), and demethylation inhibitor (DMI) fungicide groups based on amino acid sequences of the cytochrome b (cytb), β‐tubulin gene (β‐tub), and P450 sterol 14a‐demethylases (CYP51) genes. Multiple resistance of C. truncatum isolates to QoI and MBC was observed associated with mutation points in the β‐tub (E198A and F200Y) and cytb (G143A). Alternatively, low EC50 values were found for fludioxonil and difenoconazole indicating high efficacy. Analysis of C. truncatum genomes revealed two potential DMI targets, CYP51A and CYP51B, and higher genetic variability in the CYP51A gene. A positive correlation was found between genetic differentiation of C. truncatum populations and fungicide sensitivity (Student's t‐test <0.001). To our knowledge, this is the first report of multiple resistance to QoI and MBC fungicides in C. truncatum in Brazil.
Overexpression of MPV17/PMP22‐like protein 2 gene decreases production of radical oxygen species in Pyropia yezoensis (Bangiales, Rhodophyta)
Authors: Li Yujie , Yang Jiali , Sun Zhenjie , Niu Jianfeng , Wang Guangce 
Published: June 2024

AbstractThe northward shift of Pyropia yezoensis aquaculture required the breeding of germplasms with tolerance to the oxidative stress due to the high light conditions of the North Yellow Sea area. The MPV17/PMP22 family proteins were identified as a molecule related to reactive oxygen species (ROS) metabolism. Here, one of the MPV17 homolog genes designated as PyM‐LP2 was selected for functional identification by introducing the encoding sequence region/reverse complementary fragment into the Py. yezoensis genome. Although the photosynthetic activity, the respiratory rate, and the ROS level in wild type (WT) and different gene‐transformed algal strains showed similar levels under normal conditions, the overexpression (OE) strain exhibited higher values of photosynthesis, respiration, and reducing equivalents pool size but lower intracellular ROS production under stress conditions compared with the WT. Conversely, all the above parameters showed opposite variation trends in RNAi strain as those in the OE strain. This implied that the PyM‐LP2 protein was involved in the mitigation of the oxidative stress. Sequence analysis revealed that this PyM‐LP2 protein was assorted to peroxisomes and might serve as a poring channel for transferring malate (Mal) to peroxisomes. By overexpressing PyM‐LP2, the transfer of Mal from chloroplasts to peroxisomes was enhanced under stress conditions, which promoted photorespiration and ultimately alleviated excessive reduction of the photosynthetic electron chain. This research lays the groundwork for the breeding of algae with enhanced resistance to oxidative stresses.
A phenomenological inquiry into the costs and consequences of insomnia for veterans with serious mental illness
Authors: Klingaman Elizabeth A. , Lucksted Alicia , Crosby Eric S. , Kacmarek Corinne N. , Peeples Amanda , Hack Samantha , Blank Yelena , Schwartz Elana 
Published: June 2024

SummaryMany individuals with serious mental illness (i.e. schizophrenia spectrum, bipolar or major depressive disorders, with serious functional impairments) have insomnia symptoms. Insomnia is a common reason for mental health referrals in the Veterans Health Administration. The primary aim of this study was to explore the costs (what participants lose or what trade‐offs they make due to insomnia) and consequences (how insomnia impacts functioning) of insomnia for veterans with serious mental illness. Semi‐structured interviews of 20 veterans with insomnia and serious mental illness were collected as data using an inductive phenomenological approach. Two main themes were identified: Sleep Affects Mental Health and Functioning; and Compromising to Cope. Results illuminate pathways by which sleep effort destabilizes functional recovery, and illustrate how sleep has multiplicative positive impacts on functioning and mood. Researchers and clinicians alike must explore supporting people with serious mental illness in replacing sleep effort with the recovery of meaningful identity‐driven, values‐based experiences formerly conceded due to serious mental illness, insomnia or both.
Marchantia polymorphaGOLDEN2‐LIKE transcriptional factor; a central regulator of chloroplast and plant vegetative development
Authors: Hernández‐Muñoz Arihel , Agreda‐Laguna Kenny Alejandra , Ramírez‐Bernabé Ignacio E. , Oltehua‐López Omar , Arteaga‐Vázquez Mario A. , Leon Patricia 
Published: June 2024

Summary

The GOLDEN2‐LIKE (GLK) transcription factors act as a central regulatory node involved in both developmental processes and environmental responses. Marchantia polymorpha, a basal terrestrial plant with strategic evolutionary position, contains a single GLK representative that possesses an additional domain compared to spermatophytes.

We analyzed the role of MpGLK in chloroplast biogenesis and development by altering its levels, preforming transcriptomic profiling and conducting chromatin immunoprecipitation. Decreased MpGLK levels impair chloroplast differentiation and disrupt the expression of photosynthesis‐associated nuclear genes, while overexpressing MpGLK leads to ectopic chloroplast biogenesis. This demonstrates the MpGLK functions as a bona fide GLK protein, likely representing an ancestral GLK architecture.

Altering MpGLK levels directly regulates the expression of genes involved in Chl synthesis and degradation, similar to processes observed in eudicots, and causes various developmental defects in Marchantia, including the formation of dorsal structures such as air pores and gemma cups. MpGLK, also directly activates MpMAX2 gene expression, regulating the timing of gemma cup development.

Our study shows that MpGLK functions as a master regulator, potentially coupling chloroplast development with vegetative reproduction. This illustrates the complex regulatory networks governing chloroplast function and plant development communication and highlight the evolutionary conservation of GLK‐mediated regulatory processes across plant species.
Genetic analyses of embryo homology and ontogeny in the model grass Zea mays subsp. mays
Authors: Wu Hao , Zhang Ruqiang , Scanlon Michael J. 
Published: June 2024

Summary

The homology of the single cotyledon of grasses and the ontogeny of the scutellum and coleoptile as the initial, highly modified structures of the grass embryo are investigated using leaf developmental genetics and targeted transcript analyses in the model grass Zea mays subsp. mays.

Transcripts of leaf developmental genes are identified in both the initiating scutellum and the coleoptile, while mutations disrupting mediolateral leaf development also disrupt scutellum and coleoptile morphology, suggesting that these grass‐specific organs are modified leaves.

Higher‐order mutations in WUSCHEL‐LIKE HOMEOBOX3 (WOX3) genes, involved in mediolateral patterning of plant lateral organs, inform a model for the fusion of coleoptilar margins during maize embryo development.

Genetic, RNA‐targeting, and morphological evidence supports models for cotyledon evolution where the scutellum and coleoptile, respectively, comprise the distal and proximal domains of the highly modified, single grass cotyledon.
Efficacy of topical agents in oral mucositis prevention: Systematic review and network meta‐analysis
Authors: Coppini Martina , Caponio Vito Carlo Alberto , Mauceri Rodolfo , Bizzoca Maria Eleonora , Laino Luigi , Lorenzo‐Pouso Alejandro I. , Russo Diana , Troiano Giuseppe , Silva Fábio França Vieira E. , Lo Muzio Lorenzo , Campisi Giuseppina 
Published: June 2024

AbstractBackgroundOral mucositis (OM) is considered one of the most common side effects of patients undergoing cancer therapy. OM prevention plays a crucial role in the effectiveness of cancer treatment and the patient's quality of life. Different preventive treatments have been proposed in clinical trials, however with inconclusive results.Materials and MethodsA systematic review search was conducted in PubMed, Scopus, Web of Science, and Cochrane Database to answer the PICO question: in cancer patients, do specific topical agents compared to standard treatments or placebo reduce the onset and severity of oral mucositis? The risk of bias was assessed, and a network meta‐analysis was conducted.ResultsOf 2913 results, 30 randomized clinical trials were considered suitable for inclusion. A total of 2564 patients were analyzed, of which 1284 belonged to the test group and 1280 belonged to the control group. Natural products were the most used, followed mainly by antimicrobial agents, coating agents, and basic oral care measures. Topical sucralfate resulted in the most powerful intervention for the OM prevention (OR = 0.04, 95%C.I. = 0.01–0.25, p‐value = 0.001).ConclusionDue to its cytoprotective action, low cost, ease of administration, and safety, sucralfate could become a potential ally to prevent the onset of OM during cancer therapy.
Characteristics of refractive development in children aged 4 months to 8 years in urban China: A retrospective screening analysis
Authors: Yan Yarong , Xia Xuewen , Zhang Qinghui , Li Xuan 
Published: June 2024

AbstractPurposeTo conduct a large retrospective study of screening refractive error in young children.MethodsThis retrospective study included children aged from 4 months to 8 years in Daxing District, Beijing, who underwent refractive examinations without cycloplegia. It included a cross‐sectional assessment of refractive error screening for all children, and a longitudinal component for a subgroup with data available for two to five visits.ResultsA total of 14,987 children were included in the cross‐sectional study. In the group <1 year of age, the percentage of children with a spherical equivalent (SE) >+2.00 D or with cylinder <−1.50 D was 15.25% and 33.24%, respectively. These were significantly higher than for the 1‐ to 4‐year‐old group (SE 8.1% higher, cylinder 13.2% higher) (χ2 = 53.57, p < 0.001; χ2 = 790.39, p < 0.001). Furthermore, 34.83% of children in the 0‐year‐old group had amblyopia risk factors (ARFs). In the 4‐year‐old group, boys had a significantly longer axial length (AL) than girls (differences in the right and left eyes were 0.53 and 0.56 mm, respectively; z = 5.48 p < 0.001, z = 5.80, p < 0.001). AL increased with age, while the AL difference between boys and girls remained stable at 4–8 years of age. The percentage of children aged 5–8 years with myopia in 2020–2021 was significantly higher than that in 2018–2019 (H = 12.44, p = 0.006). In the longitudinal study of 4406 children (up to 12‐month follow‐up), annual changes in SE were −0.27, −0.06, 0.19 and 0.13 D between 0 and 3 years, and −0.38, −0.58, −0.70 and −0.75 D between 5 and 8 years.ConclusionsChildren's refractive error varied significantly from ages 4 months to 1 year, with a high proportion having ARFs. Children aged 5–8 years showed a trend towards myopia. The prevalence of myopia in the cross‐sectional analysis in 2020–2021 was greater than in 2018–2019. Screening refraction changed minimally over a 12‐month period for children aged 1–3 years, but became more myopic for children aged 5–8 years.