bluuebunny/crossref_metadata_2025_split huggingface dataset:
Tibia properties in broilers raised on their own commercial diets with different growth rates and sex during a 10‐week rearing period
Authors: Çapar Akyüz Hilal , Onbaşılar Esin Ebru , Yalçın Sakine 
Published: April 2024

AbstractBackgroundGenetic selection applied to broilers results in fast growth and an increase in meat yield. However, this situation causes welfare problems in broilers.ObjectivesThe aim of this study is to determine the weekly changes in the tibia characteristics in broilers raised on their own commercial diets.MethodsIn the study, 168 (84 female and 84 male) slow‐growing (Hubbard‐Isa Red JA) and 168 (84 female and 84 male) fast‐growing (Ross‐308) day‐old broiler chicks were used. Six broilers from each genotype and sex group were weighed weekly and slaughtered to determine the tibia properties during the 10‐week fattening period.ResultsFast‐growing broilers had higher tibia weight and longer length, diaphysis diameter and medullary canal diameter than those of slow‐growing broilers at the same age. In fast‐growing genotypes, the cortical index was low only in the 2nd week, and Robusticity and Seedor indices were observed to be better throughout the whole fattening. The ash content of the total tibia in the fast‐growing broilers was higher in all of the examined weeks except the 4th week and the 9th week of fattening than that in the slow‐growing broilers. Although the amount of ash per unit body weight in the 1st week of fattening was higher in fast‐growing broilers, this situation reversed after the 4th week. The level of all minerals examined in total tibia weight is high in fast‐growing broilers, and they differed according to the fattening period.ConclusionsWhen comparing tibia characteristics of two different genotypes fed their own commercial diets, the tibia structure was found to be stronger in fast‐growing broilers compared to other genotypes at the same age, but slow‐growing broilers were more prominent in terms of body weight. It was observed that the mineral density was higher in male broilers, except in the 1st week.
AI chatbots in pet health care: Opportunities and challenges for owners
Authors: Jokar Mohammad , Abdous Arman , Rahmanian Vahid 
Published: April 2024

AbstractThe integration of artificial intelligence (AI) into health care has seen remarkable advancements, with applications extending to animal health. This article explores the potential benefits and challenges associated with employing AI chatbots as tools for pet health care. Focusing on ChatGPT, a prominent language model, the authors elucidate its capabilities and its potential impact on pet owners' decision‐making processes. AI chatbots offer pet owners access to extensive information on animal health, research studies and diagnostic options, providing a cost‐effective and convenient alternative to traditional veterinary consultations.The fate of a case involving a Border Collie named Sassy demonstrates the potential benefits of AI in veterinary medicine. In this instance, ChatGPT played a pivotal role in suggesting a diagnosis that led to successful treatment, showcasing the potential of AI chatbots as valuable tools in complex cases. However, concerns arise regarding pet owners relying solely on AI chatbots for medical advice, potentially resulting in misdiagnosis, inappropriate treatment and delayed professional intervention.We emphasize the need for a balanced approach, positioning AI chatbots as supplementary tools rather than substitutes for licensed veterinarians. To mitigate risks, the article proposes strategies such as educating pet owners on AI chatbots' limitations, implementing regulations to guide AI chatbot companies and fostering collaboration between AI chatbots and veterinarians. The intricate web of responsibilities in this dynamic landscape underscores the importance of government regulations, the educational role of AI chatbots and the symbiotic relationship between AI technology and veterinary expertise.In conclusion, while AI chatbots hold immense promise in transforming pet health care, cautious and informed usage is crucial. By promoting awareness, establishing regulations and fostering collaboration, the article advocates for a responsible integration of AI chatbots to ensure optimal care for pets.
Molecular mechanisms and therapeutic strategies for neuromuscular diseases
Authors: Zambon Alberto Andrea , Falzone Yuri Matteo , Bolino Alessandra , Previtali Stefano Carlo 
Published: April 2024

AbstractNeuromuscular diseases encompass a heterogeneous array of disorders characterized by varying onset ages, clinical presentations, severity, and progression. While these conditions can stem from acquired or inherited causes, this review specifically focuses on disorders arising from genetic abnormalities, excluding metabolic conditions. The pathogenic defect may primarily affect the anterior horn cells, the axonal or myelin component of peripheral nerves, the neuromuscular junction, or skeletal and/or cardiac muscles. While inherited neuromuscular disorders have been historically deemed not treatable, the advent of gene-based and molecular therapies is reshaping the treatment landscape for this group of condition. With the caveat that many products still fail to translate the positive results obtained in pre-clinical models to humans, both the technological development (e.g., implementation of tissue-specific vectors) as well as advances on the knowledge of pathogenetic mechanisms form a collective foundation for potentially curative approaches to these debilitating conditions. This review delineates the current panorama of therapies targeting the most prevalent forms of inherited neuromuscular diseases, emphasizing approved treatments and those already undergoing human testing, offering insights into the state-of-the-art interventions.
Mapping and characterization of rust resistance genes Lr53 and Yr35 introgressed from Aegilops species
Authors: Xu Binyang , Shen Tao , Chen Hong , Li Hongna , Rehman Shams ur , Lyu Shikai , Hua Lei , Wang Guiping , Zhang Chaozhong , Li Kairong , Li Hao , Lan Caixia , Chen Guo-Yue , Hao Ming , Chen Shisheng 
Published: April 2024

Abstract
Key message
The rust resistance genes Lr53 and Yr35 were introgressed into bread wheat from Aegilops longissima or Aegilops sharonensis or their S-genome containing species and mapped to the telomeric region of chromosome arm 6BS.

Abstract
Wheat leaf and stripe rusts are damaging fungal diseases of wheat worldwide. Breeding for resistance is a sustainable approach to control these two foliar diseases. In this study, we used SNP analysis, sequence comparisons, and cytogenetic assays to determine that the chromosomal segment carrying Lr53 and Yr35 was originated from Ae.longissima or Ae. sharonensis or their derived species. In seedling tests, Lr53 conferred strong resistance against all five Chinese Pt races tested, and Yr35 showed effectiveness against Pst race CYR34 but susceptibility to race CYR32. Using a large population (3892 recombinant gametes) derived from plants homozygous for the ph1b mutation obtained from the cross 98M71 × CSph1b, both Lr53 and Yr35 were successfully mapped to a 6.03-Mb telomeric region of chromosome arm 6BS in the Chinese Spring reference genome v1.1. Co-segregation between Lr53 and Yr35 was observed within this large mapping population. Within the candidate region, several nucleotide-binding leucine-rich repeat genes and protein kinases were identified as candidate genes. Marker pku6B3127 was completely linked to both genes and accurately predicted the absence or presence of alien segment harboring Lr53 and Yr35 in 87 tetraploid and 149 hexaploid wheat genotypes tested. We developed a line with a smaller alien segment (< 6.03 Mb) to reduce any potential linkage drag and demonstrated that it conferred resistance levels similar to those of the original donor parent 98M71. The newly developed introgression line and closely linked PCR markers will accelerate the deployment of Lr53 and Yr35 in wheat breeding programs.
Prevalence of Sarcopenia and Its Defining Components in Non-alcoholic Fatty Liver Disease Varies According to the Method of Assessment and Adjustment: Findings from the UK Biobank
Authors: Freer Christine L. , George Elena S. , Tan Sze-Yen , Abbott Gavin , Scott David , Daly Robin M. 
Published: April 2024

AbstractSarcopenia may increase non-alcoholic fatty liver disease (NAFLD) risk, but prevalence likely varies with different diagnostic criteria. This study examined the prevalence of sarcopenia and its defining components in adults with and without NAFLD and whether it varied by the method of muscle mass assessment [bioelectrical impedance (BIA) versus dual-energy X-ray absorptiometry (DXA)] and adjustment (height2 versus BMI). Adults (n = 7266) in the UK Biobank study (45–79 years) with and without NAFLD diagnosed by MRI, were included. Sarcopenia was defined by the 2018 European Working Group on Sarcopenia in Older People definition, with low appendicular skeletal muscle mass (ASM) assessed by BIA and DXA and adjusted for height2 or BMI. Overall, 21% of participants had NAFLD and the sex-specific prevalence of low muscle strength (3.6–7.2%) and sarcopenia (0.1–1.4%) did not differ by NAFLD status. However, NAFLD was associated with 74% (males) and 370% (females) higher prevalence of low ASM when adjusted for BMI but an 82% (males) to 89% (females) lower prevalence when adjusted for height2 (all P < 0.05). The prevalence of impaired physical function was 40% (males, P = 0.08) to 123% (females, P < 0.001) higher in NAFLD. In middle-aged and older adults, NAFLD was not associated with a higher prevalence of low muscle strength or sarcopenia but was associated with an increased risk of impaired physical function and low muscle mass when adjusted for BMI. These findings support the use of adiposity-based adjustments when assessing low muscle mass and the assessment of physical function in NAFLD.
Genome-Scale Investigation of the Regulation of azoR Expression in Escherichia coli Using Computational Analysis and Transposon Mutagenesis
Authors: Salem Mona A. , Nour El-Din Hanzada T. , Hashem Abdelgawad M. , Aziz Ramy K. 
Published: May 2024

AbstractBacterial azoreductases are enzymes that catalyze the reduction of ingested or industrial azo dyes. Although azoreductase genes have been well identified and characterized, the regulation of their expression has not been systematically investigated. To determine how different factors affect the expression of azoR, we extracted and analyzed transcriptional data from the Gene Expression Omnibus (GEO) resource, then confirmed computational predictions by quantitative reverse transcription polymerase chain reaction (qRT-PCR). Results showed that azoR expression was lower with higher glucose concentration, agitation speed, and incubation temperature, but higher at higher culture densities. Co-expression and clustering analysis indicated ten genes with similar expression patterns to azoR: melA, tpx, yhbW, yciK, fdnG, fpr, nfsA, nfsB, rutF, and chrR (yieF). In parallel, constructing a random transposon library in E. coli K-12 and screening 4320 of its colonies for altered methyl red (MR)-decolorizing activity identified another set of seven genes potentially involved in azoR regulation. Among these genes, arsC, relA, plsY, and trmM were confirmed as potential azoR regulators based on the phenotypic decolorization activity of their transposon mutants, and the expression of arsC and relA was confirmed, by qRT-PCR, to significantly increase in E. coli K-12 in response to different MR concentrations. Finally, the significant decrease in azoR transcription upon transposon insertion in arsC and relA (as compared to its expression in wild-type E. coli) suggests their probable involvement in azoR regulation. In conclusion, combining in silico analysis and random transposon mutagenesis suggested a set of potential regulators of azoR in E. coli.
In situ X-ray and IR probes relevant to Earth science at the Advanced Light Source at Lawrence Berkeley Laboratory
Authors: Kunz Martin , Armstrong Katherine , Barnard Harold , Bechtel Hans A. , Couper Samantha C. , Kalkan Bora , Lisabeth Harry , MacDowell Alastair A. , Miyagi Lowell , Parkinson Dilworth Y. , Tamura Nobumichi , Williams Quentin 
Published: April 2024

AbstractAccess to synchrotron X-ray facilities has become an important aspect for many disciplines in experimental Earth science. This is especially important for studies that rely on probing samples in situ under natural conditions different from the ones found at the surface of the Earth. The non-ambient condition Earth science program at the Advanced Light Source (ALS), Lawrence Berkeley National Laboratory, offers a variety of tools utilizing the infra-red and hard X-ray spectrum that allow Earth scientists to probe Earth and environmental materials at variable conditions of pressure, stress, temperature, atmospheric composition, and humidity. These facilities are important tools for the user community in that they offer not only considerable capacity (non-ambient condition diffraction) but also complementary (IR spectroscopy, microtomography), and in some cases unique (Laue microdiffraction) instruments. The availability of the ALS’ in situ probes to the Earth science community grows especially critical during the ongoing dark time of the Advanced Photon Source in Chicago, which massively reduces available in situ synchrotron user time in North America.
Construct validation of the Japanese Core Outcome Measures Index and the impact of diseases on patient-reported outcome measures in preoperative patients with lumbar spinal stenosis and disk herniation: a single-center observational study
Authors: Mizoguchi Yasuaki , Akasaka Kiyokazu , Suzuki Kenta , Kimura Fumihiko , Hall Toby , Ogihara Satoshi 
Published: April 2024

Abstract
Purpose
The primary objective was to validate the construct validity of the Japanese Core Outcome Measures Index (COMI) in preoperative patients aged 60 years or older undergoing lumbar spine surgery for lumbar spinal stenosis (LSS) and lumbar disk herniation (LDH). Additionally, as a secondary aim, we explored the impact of these diseases on quality of life (QOL).

Methods
The analysis included 199 preoperative patients aged 60 and above who were scheduled for lumbar spine surgery. To assess QOL, Japanese versions of the COMI, Oswestry Disability Index (ODI), EuroQol-5 Dimension-3 Level (EQ-5D-3L), and SF-12v2 were employed. The study assessed the validity of the COMI and compared demographic and clinical characteristics between the LSS (147 cases) and LDH (52 cases) groups. It used multivariate covariance analysis (MANCOVA) to examine the impact of diseases (LSS and LDH) on each patient-reported outcome measure while considering covariates.

Results
Compared to the LSS group, the LDH group showed more difficulty with the COMI summary score (LSS/LDH [mean]: 6.9/8.1, p < 0.001), ODI score (46.8/57.4, p < 0.001), and EQ-5D utility (0.53/0.43, p < 0.001). The LDH group also reported more difficulties in the COMI-function, COMI-symptom-specific well-being, COMI-disability, ODI-personal care, ODI-social life, and SF-12v2-bodily pain subscales. MANCOVA demonstrated that these results were not influenced by covariates such as gender and medical history.

Conclusions
This study highlights the distinct impact of LSS and LDH on preoperative QOL in older patients undergoing lumbar spinal surgery. Tailored interventions are essential to address the specific challenges posed by these conditions and improve patient-centered outcomes and postoperative recovery.
Parastomal hernia repair, trying to optimize the impossible reconstruction
Authors: Maskal S. M. , Ellis R. C. , Miller B. T. 
Published: April 2024

Abstract
Purpose
Parastomal hernias are a common and challenging problem with high rates of wound complications and hernia recurrence after repair. We present our approach to optimizing parastomal hernia repair through preoperative preparation, surgical approach, and postoperative management.

Methods
Patients are carefully evaluated and optimized prior to surgery. Our typical surgical approach involves a generous midline laparotomy and retrorectus dissection followed by a posterior component separation with transversus abdominis release. We typically utilize a Sugarbaker technique for retromuscular mesh placement but also use the retromuscular keyhole or cruciate technique if there is insufficient bowel length.

Results
Previously published results from our institution include wound complication rates of up to 16% after open retromuscular parastomal hernia repair. Stoma-specific complications, such as mesh erosion in the bowel, may be attributed to the mesh placement techniques. Hernia recurrence rates range from 11 to 30% up to 2 years postoperatively.

Conclusion
We prefer an open retromuscular approach with a Sugarbaker mesh configuration to treat complex parastomal hernias. However, wound morbidity and repair failure rates remain high, and additional research is needed to optimize surgical outcomes.
Potential current and future distribution for Aedes aegypti and Aedes albopictus in Colombia: important disease vectors
Authors: Mejía-Jurado Estefanía , Echeverry-Cárdenas Emmanuel , Aguirre-Obando Oscar Alexander 
Published: April 2024

AbstractInvasive mosquitoes, Aedes aegypti (African) and Aedes albopictus (Asian), serve as global vectors for diseases such as dengue, Zika, and chikungunya, and they are currently present in Colombia. Consequently, the aim of this study was to assess the potential geographic distribution of these A. aegypti and A. albopictus vectors under both present and future environmental conditions in Colombia. To achieve this, a dataset was compiled using global records for each species, with those from Colombia being used for model validation. These records, in conjunction with layers of bioclimatic variables, were incorporated into ecological niche models. Predictions of potential distribution were made with the Maxent algorithm, using the Wallace EcoMod application for current conditions and the Java MaxEnt software for future projections, which represented three climate change scenarios (SSP245, SSP370, and SSP585) for two time periods (2041–2060 and 2081–2100). Additionally, niche overlap was calculated, and the suitable habitat range and population at risk in Colombia were quantified for current predictions. It is suggested that both species share ideal areas in the country with an overlap of 0.85, covering over 70% of the country's territory and reaching altitudes higher than 2,500 m, potentially affecting at least 45% of the population. In the future for both species it is estimated that, with increasing temperatures, the potential distribution could decrease, but they could invade areas with altitudes above 3,500 m (colder). It is concluded that, currently, A. aegypti and A. albopictus could have similar geographic distribution in Colombia and that, in the following years, climate change could lead to latitudinal and altitudinal variations in the distribution areas of these vectors.
There is no "I" in Postphenomenology
Authors: Claassen Kristy 
Published: April 2024

AbstractHuman beings are embedded in diverse social, cultural and political groups through which we make sense of our technologically mediated lived experience. This article seeks to reaffirm the postphenomenological subject as a primarily social subject. Critics maintain that the current postphenomenological framework does not adequately address the social, cultural and political context in which human-technology relations take place. In recent years, various additions to postphenomenology have been suggested in order to address this contextual deficit. In this article, I argue that a return to the phenomenological roots of postphenomenology reveals underexamined analytical tools that allow for greater socio-cultural and political sensitivity. I take Don Ihde’s supposed macroperceptual and microperceptual divide as a point of departure in claiming that postphenomenology has too hastily turned away from the subject as primarily socially situated. I draw upon the phenomenological tradition, particularly the social phenomenology of Alfred Schutz, to develop a social postphenomenological approach. This approach is informed by the Schutzian notions of action, the stock of knowledge at hand and consociates. In the resulting account, the postphenomenological schema of I—Technology—World is reconstrued as We—Technology—World.
Infant Social Attention Associated with Elevated Likelihood for Autism Spectrum Disorder: A Multi-Method Comparison
Authors: Fu Xiaoxue , Platt Emma , Shic Frederick , Bradshaw Jessica 
Published: April 2024

AbstractPurpose: The study aimed to compare eye tracking (ET) and manual coding (MC) measures of attention to social and nonsocial information in infants with elevated familial likelihood (EL) of autism spectrum disorder (ASD) and low likelihood of ASD (LL). ET provides a temporally and spatially sensitive tool for measuring gaze allocation. Existing evidence suggests that ET is a promising tool for detecting distinct social attention patterns that may serve as a biomarker for ASD. However, ET is prone to data loss, especially in young EL infants. Methods: To increase evidence for ET as a viable tool for capturing atypical social attention in EL infants, the current prospective, longitudinal study obtained ET and MC measures of social and nonsocial attention in 25 EL and 47 LL infants at several time points between 3 and 24 months of age. Results: ET data was obtained with a satisfactory success rate of 95.83%, albeit with a higher degree of data loss compared to MC. Infant age and ASD likelihood status did not impact the extent of ET or MC data loss. There was a significant positive association between the ET and MC measures of attention, and separate analyses of attention using ET and AC measures yielded comparable findings. These analyses indicated group differences (EL vs. LL) in age-related change in attention to social vs. nonsocial information. Conclusion: Together, the findings support infant ET as a promising approach for identifying very early markers associated with ASD likelihood.