bluuebunny/crossref_metadata_2025_split huggingface dataset:
Clinicopathological characteristics and prognostic factors of advanced colorectal mucinous adenocarcinoma
Authors: Yamaguchi Tomohiro , Taniguchi Hirokazu , Fujita Shin , Sekine Shigeki , Yamamoto Seiichiro , Akasu Takayuki , Kushima Ryoji , Tani Tohru , Moriya Yoshihiro , Shimoda Tadakazu 
Published: March 2012

Yamaguchi T, Taniguchi H, Fujita S, Sekine S, Yamamoto S, Akasu T, Kushima R, Tani T, Moriya Y & Shimoda T  (2012) Histopathology 61, 162–169Clinicopathological characteristics and prognostic factors of advanced colorectal mucinous adenocarcinomaAims:  Mucinous adenocarcinoma (MUC) is a histological variant of colorectal adenocarcinoma. The aim of the present study was to characterize clinicopathological features and identify prognostic factors of MUCs.Methods and results:  A total of 181 patients with MUC who underwent surgery between 1975 and 2003 were reviewed. The clinicopathological features of these patients were compared with those of 4125 non‐MUC patients. Univariate and multivariate analyses were conducted to identify significant prognostic factors in 102 patients with pT3 or pT4 tumour who underwent curative surgery. Patients with MUCs tended to present with more advanced clinical stages. The overall 5‐year survival rate of MUC patients was lower than that of non‐MUC patients; however, no prognostic difference was found when patients with the same clinical stages were compared. Multivariate analysis revealed male sex, bowel obstruction and infiltrating growth type as independent prognostic factors. Five‐year cancer‐specific survival rates for MUC patients with ≤1, 2 and 3 risk factors identified by multivariate analysis were 95.5%, 52.1% and 0.0%, respectively (P < 0.001).Conclusions:  Mucinous adenocarcinoma represents a distinct clinicopathological entity. Sex, bowel obstruction and growth patterns might be useful prognostic factors to identify patients with a high risk of recurrence after curative resection of advanced MUCs.
Combining semiquantitative measures of fibrosis and qualitative features of parenchymal remodelling to identify fibrosis regression in hepatitis C: a multiple biopsy study
Authors: Pattullo Venessa , Thein Hla‐Hla , Heathcote Elizabeth Jenny , Guindi Maha 
Published: March 2012

Pattullo V, Thein H‐H, Heathcote E J & Guindi M  (2012) Histopathology 61, 473–487Combining semiquantitative measures of fibrosis and qualitative features of parenchymal remodelling to identify fibrosis regression in hepatitis C: a multiple biopsy studyAims:  A fall in hepatic fibrosis stage may be observed in patients with chronic hepatitis C (CHC); however, parenchymal architectural changes may also signify hepatic remodelling associated with fibrosis regression. The aim of this study was to utilize semiquantitative and qualitative methods to report the prevalence and factors associated with fibrosis regression in CHC.Methods and results:  Paired liver biopsies were scored for fibrosis (Ishak), and for the presence of eight qualitative features of parenchymal remodelling, to derive a qualitative regression score (QR score). Combined fibrosis regression was defined as ≥2‐stage fall in Ishak stage (Reg‐I) or <2‐stage fall in Ishak stage with a rise in QR score (Reg‐Qual). Among 159 patients (biopsy interval 5.4 ± 3.1 years), Reg‐I was observed in 12 (7.5%) and Reg‐Qual in 26 (16.4%) patients. The combined diagnostic criteria increased the diagnosis rate for fibrosis regression (38 patients, 23.9%) compared with use of Reg‐I alone (P < 0.001). Combined fibrosis regression was observed in nine patients (50%) who achieved sustained virological response (SVR), and in 29 of 141 (21%) patients despite persistent viraemia. SVR was the only clinical factor associated independently with combined fibrosis regression (odds ratio 3.05).Conclusions:  The combination of semiquantitative measures and qualitative features aids the identification of fibrosis regression in CHC.
Disposition of desfuroylceftiofur acetamide in serum, placental tissue, fetal fluids, and fetal tissues after administration of ceftiofur crystalline free acid (CCFA) to pony mares with placentitis
Authors: MACPHERSON M. L. , GIGUÈRE S. , HATZEL J. N. , POZOR M. , BENSON S. , DIAW M. , SANCHEZ L. C , VICKROY T. W. , TELL L. , WETZLICH S. , SIMS J. 
Published: March 2012

Macpherson, M. L., Giguère, S., Hatzel, J. N., Pozor, M., Benson, S., Diaw, M., Sanchez, L. C., Vickroy, T. W., Tell, L., Wetzlich, S., Sims, J. Disposition of desfuroylceftiofur acetamide in serum, placental tissue, fetal fluids, and fetal tissues after administration of ceftiofur crystalline free acid (CCFA) to pony mares with placentitis.J. vet. Pharmacol. Therap. 36, 59–67.The objective of this study was to determine the pharmacokinetics of CCFA in mares with placentitis and evaluate the disposition of the drug in fetal fluids, fetal membranes, colostrum, and serum of foals. A secondary objective was to obtain pilot data regarding the efficacy of CCFA for improving foal survival in mares with placentitis. Twelve pregnant pony mares were enrolled in the study, inoculated withStreptococcus zooepidemicus, intracervically and assigned to one of three groups: CEFT (n = 3; administered CCFA only; 6.6 mg/kg, IM, q96h); COMBO (n = 6; administered combination therapy of CCFA, altrenogest, and pentoxifylline); UNTREAT (n = 3, no treatment). Treatment was initiated at the onset of clinical signs. Concentrations of desfuroylceftiofur acetamide (DCA), the acetamide derivative of ceftiofur and desfuroylceftiofur metabolites, were measured using high‐performance liquid chromatography. Maximum and minimum serum concentrations of DCA at steady state in treated mares were 2.40 ± 0.40 μg/mL and 1.06 ± 0.29 μg/mL, respectively. Concentration of DCA in colostrum was 1.51 ± 0.60 μg/mL. DCA concentrations in placenta and fetal tissues were very low (median = 0.03 μg/mL) and below the minimum inhibitory concentration of relevant pathogens. DCA was not detected in amniotic fluid or foal serum. Treatment did not appear to improve foal survival (CEFT: 0/3; COMBO: 2/6; UNTREAT: 2/3). Bacteria were recovered from the uterus of most mares postpartum and from blood cultures of most foals regardless of treatment.
Channelized debris‐flow deposits and their impact on turbidity currents: The Puchkirchen axial channel belt in the Austrian Molasse Basin
Authors: BERNHARDT ANNE , STRIGHT LISA , LOWE DONALD R. 
Published: March 2012

AbstractDeposits of submarine debris flows can build up substantial topography on the sea floor. The resulting sea floor morphology can strongly influence the pathways of and deposition from subsequent turbidity currents. Map views of sea floor morphology are available for parts of the modern sea floor and from high‐resolution seismic‐reflection data. However, these data sets usually lack lithological information. In contrast, outcrops provide cross‐sectional and lateral stratigraphic details of deep‐water strata with superb lithological control but provide little information on sea floor morphology. Here, a methodology is presented that extracts fundamental lithological information from sediment core and well logs with a novel calibration between core, well‐logs and seismic attributes within a large submarine axial channel belt in the Tertiary Molasse foreland basin, Austria. This channel belt was the course of multiple debris‐flow and turbidity current events, and the fill consists of interbedded layers deposited by both of these processes. Using the core‐well‐seismic calibration, three‐dimensional lithofacies proportion volumes were created. These volumes enable the interpretation of the three‐dimensional distribution of the important lithofacies and thus the investigation of sea floor morphology produced by debris‐flow events and its impact on succeeding turbidite deposition. These results show that the distribution of debris‐flow deposits follows a relatively regular pattern of levées and lobes. When subsequent high‐density turbidity currents encountered this mounded debris‐flow topography, they slowed and deposited a portion of their sandy high‐density loads just upstream of morphological highs. Understanding the depositional patterns of debris flows is key to understanding and predicting the location and character of associated sandstone accumulations. This detailed model of the filling style and the resulting stratigraphic architecture of a debris‐flow dominated deep‐marine depositional system can be used as an analogue for similar modern and ancient systems.
Functional expression of PHO1 to the Golgi and trans‐Golgi network and its role in export of inorganic phosphate
Authors: Arpat A. Bulak , Magliano Pasqualina , Wege Stefanie , Rouached Hatem , Stefanovic Aleksandra , Poirier Yves 
Published: March 2012

SummaryArabidopsis thaliana PHO1 is primarily expressed in the root vascular cylinder and is involved in the transfer of inorganic phosphate (Pi) from roots to shoots. To analyze the role of PHO1 in transport of Pi, we have generated transgenic plants expressing PHO1 in ectopic A. thaliana tissues using an estradiol‐inducible promoter. Leaves treated with estradiol showed strong PHO1 expression, leading to detectable accumulation of PHO1 protein. Estradiol‐mediated induction of PHO1 in leaves from soil‐grown plants, in leaves and roots of plants grown in liquid culture, or in leaf mesophyll protoplasts, was all accompanied by the specific release of Pi to the extracellular medium as early as 2–3 h after addition of estradiol. Net Pi export triggered by PHO1 induction was enhanced by high extracellular Pi and weakly inhibited by the proton‐ionophore carbonyl cyanide m‐chlorophenylhydrazone. Expression of a PHO1‐GFP construct complementing the pho1 mutant revealed GFP expression in punctate structures in the pericycle cells but no fluorescence at the plasma membrane. When expressed in onion epidermal cells or in tobacco mesophyll cells, PHO1‐GFP was associated with similar punctate structures that co‐localized with the Golgi/trans‐Golgi network and uncharacterized vesicles. However, PHO1‐GFP could be partially relocated to the plasma membrane in leaves infiltrated with a high‐phosphate solution. Together, these results show that PHO1 can trigger Pi export in ectopic plant cells, strongly indicating that PHO1 is itself a Pi exporter. Interestingly, PHO1‐mediated Pi export was associated with its localization to the Golgi and trans‐Golgi networks, revealing a role for these organelles in Pi transport.
A null mutation of ROS1a for DNA demethylation in rice is not transmittable to progeny
Authors: Ono Akemi , Yamaguchi Katsushi , Fukada‐Tanaka Sachiko , Terada Rie , Mitsui Toshiaki , Iida Shigeru 
Published: March 2012

SummaryGenes that promote DNA methylation and demethylation in plants have been characterized mainly in Arabidopsis. Arabidopsis DNA demethylation is mediated by bi‐functional DNA enzymes with glycosylase activity that removes 5‐methylcytosine and lyase activity that nicks double‐stranded DNA at an abasic site. Homologous recombination‐promoted knock‐in targeting of the ROS1a gene, the longest of six putative DNA demethylase genes in the rice genome, by fusing its endogenous promoter to the GUS reporter gene, led to reproducibly disrupted ROS1a in primary (T0) transgenic plants in the heterozygous condition. These T0 plants exhibited no overt morphological phenotypes during the vegetative phase, and GUS staining showed ROS1a expression in pollen, unfertilized ovules and meristematic cells. Interestingly, neither the maternal nor paternal knock‐in null allele, ros1a‐GUS1, was virtually detected in the progeny; such an intransmittable null mutation is difficult to isolate by conventional mutagenesis techniques that are usually used to identify and isolate mutants in the progeny population. Even in the presence of the wild‐type paternal ROS1a allele, the maternal ros1a‐GUS1 allele caused failure of early‐stage endosperm development, resulting in incomplete embryo development, with embryogenesis producing irregular but viable embryos that failed to complete seed dormancy, implying non‐equivalent maternal and paternal contribution of ROS1a in endosperm development. The paternal ros1a‐GUS1 allele was not transmitted to progeny, presumably because of a male gametophytic defect(s) prior to fertilization. Thus, ROS1a is indispensable in both male and female gametophytes, and DNA demethylation must plays important roles in both gametophytes.
Liver transplantation for a pediatric patient with hemophilia B
Authors: Sanada Yukihiro , Urahashi Taizen , Ihara Yoshiyuki , Wakiya Taiichi , Okada Noriki , Yamada Naoya , Mizuta Koichi 
Published: March 2012

Sanada Y, Urahashi T, Ihara Y, Wakiya T, Okada N, Yamada N, Mizuta K. Liver transplantation for a pediatric patient with hemophilia B.  Pediatr Transplantation 2012: 00: 000–000. © 2012 John Wiley & Sons A/S.Abstract:  Hemophilia exposes patients to greater risks of bleeding complications during the perioperative period. However, there are no current protocols for factor replacement during LT. We herein describe a case of pediatric living donor LDLT performed for a patient with hemophilia B using perioperative short‐term factor replacement. A 4‐yr‐old female patient with an extrahepatic portosystemic shunt and asymptomatic hemophilia B (factor IX activity 18.7%) underwent an ABO‐compatible LDLT using a left lobe graft. The bleeding volume was 2980 mL. Freeze‐dried human blood coagulation factor IX concentrate (Novact M, Kaketsuken, Japan) was administered at the induction of anesthesia and at the end of LDLT by bolus infusion (80 U/kg) and was continued by bolus infusion (40 U/kg) on POD 1, 2, 3, and 4. On POD 1, 5, 8, and 12, the factor IX plasma levels were 34.5%, 64.9%, 43.5%, and 53.1%, respectively. The postoperative course was uneventful, and the patient is currently doing well at 2.5 yr after LDLT. Factor concentrate should be administered at the induction of anesthesia and at the end of LT by bolus infusion, and thereafter be continued for a few days after LT by bolus infusion.
Shortening tobacco life cycle accelerates functional gene identification in genomic research
Authors: Ning G. , Xiao X. , Lv H. , Li X. , Zuo Y. , Bao M. 
Published: March 2012

AbstractDefinitive allocation of function requires the introduction of genetic mutations and analysis of their phenotypic consequences. Novel, rapid and convenient techniques or materials are very important and useful to accelerate gene identification in functional genomics research. Here, over‐expression of PmFT (Prunus mume), a novel FT orthologue, and PtFT (Populus tremula) lead to shortening of the tobacco life cycle. A series of novel short life cycle stable tobacco lines (30–50 days) were developed through repeated self‐crossing selection breeding. Based on the second transformation via a gusA reporter gene, the promoter from BpFULL1 in silver birch (Betula pendula) and the gene (CPC) from Arabidopsis thaliana were effectively tested using short life cycle tobacco lines. Comparative analysis among wild type, short life cycle tobacco and Arabidopsis transformation system verified that it is optional to accelerate functional gene studies by shortening host plant material life cycle, at least in these short life cycle tobacco lines. The results verified that the novel short life cycle transgenic tobacco lines not only combine the advantages of economic nursery requirements and a simple transformation system, but also provide a robust, effective and stable host system to accelerate gene analysis. Thus, shortening tobacco life cycle strategy is feasible to accelerate heterologous or homologous functional gene identification in genomic research.
Effects of salicylic acid, 1‐aminocyclopropan‐1‐carboxylic acid and methyl jasmonate on the frequencies of endophytic fungi in Quercus serrata leaves
Authors: Kusumoto D. , Matsumura E. 
Published: March 2012

SummaryTo examine the effects of salicylic acid (SA), the ethylene precursor 1‐aminocyclopropan‐1‐carboxylic acid (ACC) and methyl jasmonate (MJ), which induce defence responses against pathogen in plants, on foliar endophytic fungi, we performed exogenous treatments of Quercus serrata leaves with these chemicals and subsequently isolated endophytic fungi. SA and ACC significantly reduced the isolation frequency of the most dominant fungus, a Xylaria species as identified by sequence analysis of the internal transcribed spacer (ITS) region of the ribosomal DNA. In contrast, the frequency of the second most frequent fungus increased by SA and ACC. A few other fungi were also affected positively or negatively. Shannon diversity indices decreased by all treatments because of the uneven isolation frequencies of the endophytes. Our results imply that host defence responses induced by the chemical treatments reduced the number of infections of some endophytes while the frequency of some other endophytes increased benefiting from the reduction of competitors.
Body phosphorus mobilization and deposition during lactation in dairy cows
Authors: Elizondo Salazar J. A. , Ferguson J. D. , Beegle D. B. , Remsburg D. W. , Wu Z. 
Published: March 2012

SummaryDairy cow bone phosphorus (P) mobilization and deposition and their influence on P requirements were studied over the lactation cycle. Thirty Holsteins received a common diet during the dry period and one of the following three dietary treatments that varied in P percentage during the subsequent lactation (44 weeks): (i) 0.36 throughout (constant P, 0.36‐0.36‐0.36), (ii) 0.36 for 30 weeks then 0.29 for 14 weeks (P changed once, 0.36‐0.36‐0.29), and (iii) 0.43 for 10 weeks, 0.36 for 20 weeks, and 0.29 for 14 weeks (P changed twice, 0.43‐0.36‐0.29). Six P balance studies were conducted during the experiment, including one during the dry period and five along lactation, based on P intake, faecal P, urinary P and milk P, when appropriate. Blood samples were taken during balance to analyse bone formation (osteocalcin) and resorption (pyridinoline) marker concentrations and rib biopsies performed to determine bone P content. Phosphorus balance was negative during weeks −4 to −1 relative to lactation for all groups and remained negative for cows fed 0.36% P during weeks 1–5, but showed a positive value for cows that received 0.43% P. The balance was close to zero for all groups at weeks 19–23 and showed a clear retention during weeks 38–42; by the end of lactation, cows re‐stored most of the P mobilized earlier. The pattern in P balance was consistent with changes in blood bone metabolism marker concentrations, rib bone P content, and faecal and urinary P concentrations over the experiment, indicating that cows, irrespective of the dietary P treatments received, mobilized P from bone during the late dry period when fed a low‐Ca diet and early lactation, and re‐stored P in late lactation. This dynamic of P metabolism can have important implications for dietary P requirements and ration formulations.
High connectivity between sea lough populations of a planktonic larval disperser with the adjacent open coast
Authors: Bell James J. 
Published: March 2012

AbstractSeveral previous studies have reported high levels of genetic differentiation between populations inside European Marine Reserves and their adjacent coastlines for a direct‐developing species. These studies suggest that sea loughs may not represent the most suitable sites for inclusion into Marine Protected Area (MPA) or Marine Reserve networks as they are not well connected to other populations. However, many marine species have pelagic larvae, and the connectivity of populations of such species inside sea loughs with non‐lough populations remains unknown. Here, I investigated the levels of gene flow between populations of the acorn barnacle Semibalanas balanoides inside and outside Strangford Lough, Northern Ireland; this species has a long‐lived pelagic larvae (4–6 weeks). There were no significant differences in the levels of genetic diversity between sea lough and non‐lough populations and no evidence for genetic differentiation between any of the populations examined; the populations appear well mixed and well connected, in contrast to what has been reported for a previous direct‐developing species.
Post‐Warming Competence of In Vivo Matured Rabbit Oocytes Treated with Cytoskeletal Stabilization (Taxol) and Cytoskeletal Relaxant (Cytochalasin B) Before Vitrification
Authors: Jiménez‐Trigos E , Naturil‐Alfonso C , Vicente JS , Marco‐Jiménez F 
Published: March 2012

ContentsThe aim of this study was to investigate the effect of Taxol and Cytochalasin B on the spindle, chromosome configuration and development to blastocyst stage after parthenogenesis activation of in vivo matured rabbit oocytes after vitrification. Oocytes were randomized into four groups: oocytes treated with Cytochalasin B or Taxol before vitrification, oocytes without treatment before vitrification and fresh oocytes. Oocytes were vitrified using Cryotop method, and meiotic spindle and chromosomal distribution were assessed with a confocal laser scanning microscopy. To determine oocyte competence, in vitro development of oocytes was assessed with parthenogenesis activation. There were no significant differences in the frequencies of normal spindle (33.0%, 31.0% and 32.6%, for non‐treated, Taxol‐treated and Cytochalasin B‐treated oocytes, respectively) and chromosome (48.3%, 46.6% and 34.8%, for non‐treated, Taxol‐treated oocytes and Cytochalasin B‐treated oocytes respectively) in vitrified groups, but significantly lower than those of fresh group (89.7% and 90.2%, for normal spindle and chromosome organization, respectively). No statistical differences were found in the cleavage and blastocyst development rates between non‐treated and Taxol‐treated oocytes (7.7% and 1.5% and 13.7% and 4.6%, for non‐treated and Taxol‐treated oocytes, respectively), although they were significantly lower than in the fresh group (42.3% and 32.1%, for cleavage and blastocyst development, respectively). Oocytes treated with Cytochalasin B failed to reach blastocyst stage. Normal spindle, chromosome configuration and blastocyst development of in vivo matured rabbit oocytes were damaged in vitrification, which was not improved by Taxol and Cytochalasin B pre‐treatment before vitrification. Moreover, a detrimental effect on blastocyst development of Cytochalasin B pre‐treatment before vitrification was observed.
Blood‐borne viruses in the haemodialysis‐dependent population attending Top End Northern Territory facilities 2000–2009
Authors: DAVIES JANE , JABBAR ZULFIKAR , GAGAN FIZZA , BAIRD ROBERT W 
Published: March 2012

ABSTRACTAim:  To describe the incidence and prevalence of blood‐borne viruses (BBV) including: hepatitis B virus (HBV), hepatitis C virus (HCV), human immunodeficiency virus (HIV) and human T‐cell leukaemia virus type‐1 (HTLV) in the haemodialysis‐dependent population of the Top End of the Northern Territory (TENT).Methods:  We retrospectively reviewed the serology of BBV in a longitudinal fashion in the haemodialysis‐dependent population treated in the TENT of Australia from 2000 to 2009 inclusive. HBV, HCV, HIV and HTLV serology on commencement of dialysis and at exit or January 2010, whichever was earlier, as well as demographic details were collected. Patients with a change in serological status had all serology reviewed.Results:  Four‐hundred and forty patients were included in the analysis. Of these, 84.3% were Indigenous and 55.4% female, with a median age of 50 (IQR 43–59) years at the commencement of haemodialysis. Evidence of past HBV infection was documented in 42.7% and 8.9% were hepatitis B surface antigen‐positive. Positive serology for HTLV was documented in 2.2%, 1.6% were hepatitis C antibody‐positive and no individual was HIV‐positive. Three patients had a definite change in their HBV serology over time; this equates to an absolute seroconversion risk of 0.1 per 100 person years or 0.0006 per dialysis episode.Conclusions:  In this cohort, there was a high rate of past and current hepatitis B infection but low rates of seroconversion while on haemodialysis.