bluuebunny/crossref_metadata_2025_split huggingface dataset:
Wheat root exudates suppress faba bean Fusarium wilt disease
Authors: Yang Siyin , Zheng Yiran , Cen Zixuan , Guo Yuting , Yang Zhixian , Dong Yan 
Published: June 2024

AbstractContinuous cultivation of faba beans often results in a high occurrence of Fusarium wilt. Nevertheless, this issue can be successfully managed through wheat‐faba bean intercropping. Our objective is to elucidate the function of non‐host wheat in combating faba bean Fusarium wilt. We assessed the impact of wheat on the development of faba bean Fusarium wilt through field and pot experiments, and examined and identified the compounds in the root exudates of wheat. The influence of wheat root exudates on Fusarium commun mycelial growth and spore multiplication was investigated through indoor culture experiments. Wheat‐faba bean intercropping significantly reduced the occurrence of Fusarium wilt in field trials. Root separation experiments in pots indicated that wheat root exudates might play a vital role in this outcome. The exogenous addition of wheat root exudates to faba bean grown alone notably decreased the occurrence and severity of Fusarium wilt. Furthermore, the exogenous addition of wheat root exudates effectively hindered the growth and reproduction of Fusarium commun's mycelium. And, wheat root exudates contained six compounds with relatively high concentrations, including salicylic acid, p‐hydroxybenzoic acid, tartaric acid, malic acid, glutamic acid, and threonine, all of which inhibited the growth and spore formation of Fusarium commun. In a wheat‐faba bean intercropping system, these six compounds found in the root exudate of non‐host wheat can help control Fusarium wilt in faba bean by inhibiting the mycelial growth and sporulation of Fusarium commun.
Evaluation of raw milk samples and vendor‐derived Staphylococcus aureus and Coxiella burnetii prevalence in dairy delicatessens in eastern Turkey
Authors: Kaplan Muhammed Furkan , Kaplan Ece , Raza Ali , Demirler Mehtap , Baran Alper , Cengiz Seyda , Adiguzel Mehmet Cemal 
Published: June 2024

AbstractThis study investigated the prevalence, antimicrobial resistance, and genetic diversity of Staphylococcus aureus, as well as the detection and genetic relationship of Coxiella burnetii in retail milk samples and plastic bags from 25 local dairy delicatessens. Bacteriological culture, PCR, and Sanger sequencing were performed. Eleven percent of the samples were positive for S. aureus, none of which were methicillin‐resistant or enterotoxigenic. The rep‐PCR analysis revealed a high similarity between strains isolated from milk and bag samples from the same source. Moreover, 14% of the milk samples were positive for C. burnetii, which matched 100% of the reference strains in the GenBank.
Endometrial microbial dysbiosis and metabolic alteration promote the development of endometrial cancer
Authors: Han Xinxin , Zheng Jia , Zhang Lizhi , Zhao Zhongwei , Cheng Guangyan , Zhang Wenwen , Qu Pengpeng 
Published: June 2024

AbstractObjectiveEmerging evidence suggests that the endometrial microbiome plays important roles in the development of endometrial cancer (EC). Here, we evaluate stage‐specific roles of microbial dysbiosis and metabolic disorders in patients with EC, patients with endometrial hyperplasia (EH), and patients afflicted with benign uterine conditions (CK).MethodsThis prospective cohort study included 33 women with EC, 15 women with endometrial EH, and 15 women with benign uterine conditions (CK) from November 2022 to September 2023. Different typical endometrial samples were imaged with a scanning electron microscope and a transmission electron microscope. The endometrial microbiome was assessed by sequencing the V3–V4 region of the 16S rRNA gene and the ITS1 to fill the gap in relation to the study of the uterine fungal microbiome. Moreover, liquid chromatography‐mass spectrometry‐based metabolomics was used to identify and quantify metabolic changes among these groups.ResultsThe endometrial microbiome revealed that there is a structural microbiome shift and an increase in the α‐diversity in the EC and EH cases, distinguishable from the benign cases, especially the fungal community structure. The fungal microbiome from patients with EC and EH was altered relative to controls and dominated by Penicillium sp. By contrast, Sarocladium was more abundant in controls. Significant differences were observed in the composition and content of compounds between benign cases and EC, especially estradiol‐like metabolism‐related substances. Altered microbiota was correlated with the concentrations of interleukin‐6 (IL‐6), IL‐11, transforming growth factor‐beta, and β‐glucuronidase activity especially the relative abundance increase of Penicillium sp.ConclusionsThis study suggested that the endometrial microbiome is complicit in modulating the development of EC such as estrogen activity and a pro‐inflammatory response. Our work provides a new insight into the endometrial microbiome from a perspective of stages, which opens up new avenues for EC prognosis and therapy.
Photocrosslinkable hydrogel of ibuprofen‐chitosan methacrylate modulates inflammatory response
Authors: Guan Xiangheng , Yao Haochen , Wu Jinglei 
Published: June 2024

AbstractMethacrylated biopolymers are unique and attractive in preparing photocrosslinkable hydrogels in biomedical applications. Here we report a novel chitosan (CS) derivative‐based injectable hydrogel with anti‐inflammatory capacity via methacrylation modification. First, ibuprofen (IBU) was conjugated to the backbone of CS by carbodiimide chemistry to obtain IBU‐CS conjugate, which converts water‐insoluble unmodified CS into water‐soluble IBU‐CS conjugate. The IBU‐CS conjugate did not precipitate at the pH of 7, which was beneficial to subsequent chemical modification with methacrylic anhydride to prepare IBU‐CS methacrylate (IBU‐CS‐MA) with significantly higher methacrylation substitution. Photocrosslinkable in situ gel formation of injectable IBU‐CS‐MA hydrogel was verified using lithium phenyl‐2,4,6‐trimethylbenzoylphosphinate (LAP) initiator under visible light. The IBU‐CS‐MA hydrogel showed good cytocompatibility as revealed by encapsulating and in vitro culturing murine fibroblasts within hydrogels. It promoted macrophage polarization toward M2 phenotype, as well as downregulated pro‐inflammatory gene expression and upregulated anti‐inflammatory gene expression of macrophages. The hydrogel also significantly reduced the reactive oxygen specifies (ROS) and nitrogen oxide (NO) produced by lipopolysaccharides (LPS)‐stimulated macrophages. Upon subcutaneous implantation in a rat model, it significantly mitigated inflammatory responses as shown by significantly lower inflammatory cell density, less cell infiltration, and much thinner fibrous capsule compared with CS methacrylate (CS‐MA) hydrogel. This study suggests that IBU‐CS conjugate represents a feasible strategy for preparing CS‐based methacrylate hydrogels for biomedical applications.
Bleaching effect on bonding performance of composite to enamel
Authors: Miljkovic Milan , Dacic Stefan , Mitic Vladimir , Jovanovic Milica , Andjelkovic‐Apostolovic Marija 
Published: June 2024

AbstractThis study aimed to assess the effects of dental bleaching on the immediate shear bond strength (SBS) of composite to enamel. Different bleaching methods and time intervals between bleaching and composite restoration were examined. A total of 45 enamel specimens were divided into three groups (n = 15) based on the applied bleaching protocol: in‐office (A), at‐home (B), and unbleached control (C). Groups A and B were then subdivided into three time intervals until bonding: 1, 3, and 7 days. The composite was bonded to enamel using self‐etch adhesive technique and submitted to SBS testing. The enamel surface and adhesive interface were observed under scanning electron microscope (SEM). The highest average SBS was measured in the control (unbleached) group (31.1 ± 3.4). A significant difference in SBS was found between the control and in‐office bleaching (p < .001), as well as between control and at‐home bleaching (p = .034), while the difference between in‐office and at‐home bleaching methods was insignificant. In relation to the waiting period, a significant reduction in SBS (p < .001) was found 1 day post‐bleaching. The weakest marginal sealing to enamel under SEM was observed also 1 day after bleaching. Both bleaching methods significantly reduced the SBS of composite to enamel, especially immediately after the bleaching procedure. Reduction in SBS is temporary and 3‐day waiting period seems to be sufficient to reverse the adverse effects of bleaching.Research Highlights
SBS of composite to enamel is significantly reduced by bleaching procedures, especially immediately after bleaching.
The reduction in SBS is temporary and reversible by delaying the restoration for a minimum of 3 days.
Lysophosphatidic acid promotes ESCC progression by increasing the level of CCL2 secreted by esophageal epithelial cells
Authors: Ma Hui , Ma Xiaoqian , Qi Lingyu , Zhang Qian , Wang Tiange , Guo Qingdong , Li Peng , Zhang Shutian , Liu Si 
Published: June 2024

AbstractBackgroundLysophosphatidic acid (LPA) is a small bioactive lipid which acts as a potent regulator in various tumor progressions through six G‐protein‐coupled receptors (LPA1–LPA6). Our previous study demonstrated that the LPA‐producing enzyme, autotaxin (ATX), was upregulated in esophageal squamous cell carcinoma (ESCC) and ATX high expression levels indicated a poor prognosis. Esophageal squamous cell carcinoma is a type of malignant tumor which originates from epithelial cells. Its progression can be affected by the interaction between cancer cells and normal cells. However, the impact of LPA on the interaction between esophageal epithelial cells and cancer cells in the development of ESCC remains uncertain.MethodsMTS and Edu assays were performed to determine ESCC cell proliferation in culture medium (CM) derived from LPA‐stimulated esophageal epithelial cells (Het‐1a). A wound healing assay, transwell migration and an invasion assay were performed to assess the metastatic ability of ESCC cells. Cytokine array analysis was conducted to detect the differentially secreted cytokines in CM. The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases were utilized to uncover the pathways and cytokines that are influenced by LPA in ESCC. Immunohistochemical staining was employed to measure the expression of ATX and CCL2 in early‐stage ESCC. Quantitative real‐time PCR, western blot, enzyme‐linked immunosorbent assay and an antibody neutralization assay were employed to measure the mechanism of LPA‐mediated communication between epithelial cells and cancer cells.ResultsFunctional experiments showed that exposing ESCC cancer cells to CM from LPA‐treated Het‐1a results in promoting proliferation, migration, invasion and epithelial–mesenchymal transition processes. Using cytokine array analysis, we discovered that LPA triggers the release of multiple cytokines from epithelial cells. After screening of the TCGA and GEO databases, CCL2 was identified and found to be correlated with ATX expression in ESCC. Furthermore, CCL2 levels in both mRNA expression and secretion were observed to be upregulated in epithelial cells upon stimulation with LPA. Blocking CCL2 effectively reduced the pro‐migration influence of CM derived from LPA‐treated Het‐1a. Mechanism studies have demonstrated that LPA activated the NF‐κB signaling pathway through LPA1/3, ultimately causing an increase in CCL2 expression and secretion in Het‐1a.ConclusionsOur findings, taken together, demonstrate that CM from LPA‐treated esophageal epithelial cells plays a significant role in promoting the progression of ESCC, with CCL2 acting as the primary regulator.
Clinical significance of ribosomal protein S15 expression in patients with colorectal cancer liver metastases
Authors: Sakano Yoshihiro , Matoba Daijiro , Noda Takehiro , Kobayashi Shogo , Yamada Daisaku , Tomimaru Yoshito , Takahashi Hidenori , Uemura Mamoru , Doki Yuichiro , Eguchi Hidetoshi 
Published: June 2024

AbstractBackgroundLiver metastasis is the most frequently observed distant metastasis of colorectal cancer, and the residual liver recurrence rate after hepatic resection is still high. To explore the mechanism of liver metastasis to discover potential new treatments, we assessed the relationship between the expression of differentially expressed genes (DEGs) and prognosis in patients with colorectal cancer liver metastasis (CRLM).MethodsThe gene expression dataset was extracted from The Cancer Genome Atlas and the Gene Expression Omnibus. Significance analysis of DEGs between tumor and normal samples of colorectum, liver, and lung was conducted. A total of 80 CRLM patients were studied to assess the expression of RPS15, characteristics, and outcomes. We examined the relationships of RPS15 expression to cell viability and apoptosis in vitro and vivo.ResultsSignificance analysis identified 33 DEGs. In our cohorts, the overall survival rates were significantly lower in the high‐RPS15‐expression group, and high expression of RPS15 was an independent and unfavorable prognostic factor in recurrence‐free survival and overall survival. Knockdown of RPS15 expression reduced the proliferative capacity of colorectal cancer cells and increased BAX‐induced apoptotic cell death.ConclusionsRPS15 expression is an independent prognostic factor for CRLM patients and might be a novel therapeutic target for CRLM.
Addressing food insecurity in the inpatient setting: Results of a postdischarge pilot study
Authors: Smith Megan , Tepe Kerry A. , Sauers‐Ford Hadley , Atarama Denise , Gilliam Monique , Unaka Ndidi , Beck Andrew F. , Shah Anita N. , Schondelmeyer Amanda C. , Auger Katherine A. 
Published: June 2024

AbstractBackground and ObjectivesWith a growing interest in screening for food insecurity (FI) during pediatric hospitalization, there is a parallel need to develop interventions. With input from caregivers experiencing FI, we sought to identify interventions to assist with short‐term FI after discharge and evaluate their feasibility, acceptability, and appropriateness.MethodsWe first employed qualitative methods to identify potential interventions. Next, we conducted a pilot study of selected interventions for families experiencing FI. Seven days postdischarge, caregivers rated the intervention's feasibility, acceptability, and appropriateness. We also assessed for ongoing FI. We summarized the median and proportion of “completely agree” responses to feasibility, acceptability, and appropriateness questions, and we compared in‐hospital and postdischarge FI using McNemar's test.ResultsIn the qualitative stage, 14 caregivers prioritized three interventions: grocery store gift cards, grocery delivery/pick‐up, and frozen meals. In the pilot study, 53 caregivers (25% of those screened) endorsed FI during their child's hospitalization and received one or more of the interventions. Every caregiver selected the grocery gift card option; 37 families (69.8%) also received frozen meals. Seven days after discharge, most caregivers rated the intervention as “completely” feasible (76%), acceptable (90%), and appropriate (88%). There was a significant decrease in caregivers who reported FI after discharge compared to during the hospitalization (p < .001).ConclusionsThis study demonstrates the feasibility, acceptability, and appropriateness of inpatient interventions to address FI, particularly at the time of pediatric hospital discharge and transition home. Randomized trials are needed to further evaluate the efficacy of interventions employed during hospitalization.
Evaluation of antioxidant, antimicrobial, and bioactive properties and peptide sequence composition of Malatya apricot kernels
Authors: Aydın Çağlar Mert , Çelikbıçak Ömür , Hayaloğlu Ali Adnan 
Published: June 2024

AbstractBACKGROUNDThis study used four different apricot (Prunus armeniaca) kernels cultivated in Malatya during two consecutive years. The varieties were Hacihaliloglu, Hasanbey, Kabaasi, and Zerdali. The physicochemical properties of the kernels were determined, and the bioactive content of the kernels was evaluated using kernel hydrolysates prepared using trypsin.RESULTSWith regard to the physicochemical properties of the kernels, the dry matter ratio and protein content were the highest in the Hacihaliloglu variety; the ash ratio was the highest in the Kabaasi variety, and the free oil ratio was the highest in the Hasanbey variety. The bioactive compound content changed according to kernel variety. Angiotensin‐converting enzyme inhibitors activity was found to be the highest in the Hacihaliloglu and Hasanbey varieties, which had the lowest amygdalin content, and Zerdali had the highest amygdalin content. The antioxidant and antimicrobial effects of the kernels varied, with Hasanbey and Kabaasi generally having the highest content in both analyses. Moreover, a concentration of 20 mg mL−1 of the hydrolysate was determined to have a destructive effect for the microorganisms used in this study. The storage protein of the kernels, except Hacihaliloglu, was found to be Prunin 1, with the longest matching protein chain in the kernels being R.QQQGGQLMANGLEETFCSLRLK.E.CONCLUSIONThe results suggest that the peptide sequences identified in the kernels could have antihypertensive, antioxidative, and Dipeptidyl peptidase IV (DPP‐IV) inhibitory effects. Consequently, apricot kernels show potential for use in the production of functional food products. Of the kernels evaluated in this study, Hacihaliloglu and Hasanbey were deemed the most suitable varieties due to their higher bioactive content and lower amygdalin content. © 2024 The Author(s). Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.
Quick Response Auto‐Coding and Recognition of Mixed Vapors Via Microlaser Array Sensor
Authors: Shan Hongrui , Wei Qiheng , Dai Hailang , Wang Xueqian , Yang Hong , Chen Xianfeng 
Published: June 2024

AbstractThe superior stimuli‐responsiveness, narrow linewidth, and high spectral multiplexing capacity of microlasers have led to their use as photonic tags for molecular labeling, encryption, and anticounterfeiting. However, the requirement of consistent lasing features for repeated measurements and the need for lasing features to change regularly with varying analytes pose a challenge to the efficient and convenient authentication of laser‐encoded photonic tags for practical applications. To address this challenge, an optical microsphere array is proposed that provides a set of real‐time typical lasing spectra collected from microspheres coated with specific recognition surface films of different sizes capable of recognizing one analyte or a mixture of analytes. These lasing spectra were transformed into 2D grayscale barcodes. Additionally, a gray value‐quick response code (GV‐QR code) is developed using deep learning methods, which enables the real‐time monitoring and identification of molecular concentration changes through GV‐QR autocoding, resulting in more precise, wide‐ranging, and reliable molecular detection.
The Influence of the Methylene Blue Dye on the Rate and the Mechanism of the Oxidative Polymerization of Aniline
Authors: Mezhuev Yaroslav , Motyakin Mikhail , Vorobev Igor , Ionova Irina , Baranov Oleg , Dvorikova Raisa , Tsatsakis Aristidis , Soldatova Anastasia , Svistunova Alina 
Published: June 2024

AbstractIt is shown that one‐electron oxidation of aniline with methylene blue is possible only at low temperatures under UV radiation. The kinetics of aniline polymerization under the action of ammonium persulfate in the presence of catalytic amounts of methylene blue is studied. Kinetic curves are obtained by electron paramagnetic resonance (EPR) spectroscopy at various temperatures and concentrations of methylene blue. The catalytic action of the unassociated form of methylene blue and the deactivation of reactive complexes by the dye dimers are shown to compete. In the context of the obtained results the autocatalysis mechanism of oxidative polymerization is considered, which suggests the formation of complexes of aniline and polyaniline that are subject to subsequent rapid oxidation by the persulfate anion.
Design, Synthesis, and Performance of Photo‐Responsive Liquid Crystal Polymers with Stepwise Deformation Capability
Authors: Gui Qin , Liu Zui , Sun Xiangling , Guo Guangqiang , Yuan Yongjie , Zhang Hailiang 
Published: June 2024

AbstractPhoto‐responsive liquid crystal polymers (LCPs) have potential application value in flexible robots, artificial muscles, and microfluidic control. In recent years, significant progress has been made in the development of LCPs. However, the preparation of LCPs with continuous and controllable stepwise deformation capabilities remains a challenge. In this study, visible photo‐responsive cyanostilbene monomer, UV photo‐responsive azobenzene monomer, and multiple hydrogen bond crosslinker are used to prepare photo‐responsive LCPs capable of achieving continuously and controllable stepwise deformation. The comprehensive investigation of the multiple light response ability and photo‐induced deformation properties of these copolymers is conducted. The results reveal that in the first stage of photo‐induced deformation under 470 nm blue light irradiation, the deformation angle decreases with a reduction in cyanostilbene content in the copolymer component, ranging from 40° in AZ0‐CS4 to 0° in AZ4‐CS0. In the second stage of photo‐induced deformation under 365 nm UV irradiation, the deformation angle increases with the increase of azobenzene content, ranging from 0° of AZ0‐CS4 to 89.4° of AZ4‐CS0. Importantly, the deformation between these two stages occurs as a continuous process, allowing for a direct transition from the first‐stage to the second‐stage deformation by switching the light source from 470 to 365 nm.
Elevating haptic interfaces: Dual‐rate sampling and field programmable gate array implementation for multi‐degree‐of‐freedom performance enhancement
Authors: Koul Majid , Khosa Suhail , Ahmad Babar 
Published: June 2024

AbstractIn this work, our primary focus centered on exploring the adaptability of the dual‐rate sampling scheme proposed earlier to enhance the performance of multi‐degree‐of‐freedom (multi‐DOF) impedance‐based haptic interfaces. The scheme employed independent sampling rates in a haptics controller, effectively mitigating the issue of reduced Z‐width at higher sampling rates. A key aspect of our investigation was the intricate implementation of the dual‐rate sampling scheme on a field programmable gate array (FPGA). This implementation on a logic hardware FPGA was challenging and led to the effective comparison of the uniform‐rate and dual‐rate sampling schemes of the multi‐DOF haptic controller. We used an in‐house developed two‐DOF pantograph as the haptic interface and an FPGA for implementing the controller strategy. FPGA‐based implementation presented challenges that were vital in testing controller performances at higher sampling rates. Virtual wall experiments were conducted to determine the stable and unstable interactions with the virtual wall. To complement the experimental results, we simulated the haptics force law for multi‐DOF system on Simulink/MATLAB. Notably, the dual‐rate sampling approach maintained the Z‐width of the two‐DOF haptic interface, even at higher controller sampling rates, distinguishing it from the conventional two‐DOF uniform‐rate control scheme. For example, employing a dual‐rate sampling combination of 20–2 kHz consistently ensured the stable rendering of a maximum virtual stiffness of approximately 700 N/mm and maintained a reliable virtual damping range spanning from 0 to 5 Ns/mm. In contrast, the 20 kHz uniform‐rate sampling approach failed to ensure interface stability in the presence of virtual damping, ultimately resulting in the unsuccessful implementation of any virtual stiffness at higher sampling rates. This work, therefore, establishes the potential of dual‐rate sampling in the realm of haptic technology, with practical applications in multi‐DOF systems.