bluuebunny/crossref_metadata_2025_split huggingface dataset:
Functional Analysis of a Cellular MicroRNA in Insect Host-Ascovirus Interaction
Authors: Hussain Mazhar , Asgari Sassan 
Published: October 2009

ABSTRACT
MicroRNAs (miRNAs) have emerged as key regulators in many biological processes, from development to defense, at almost all organismal levels. Recently, their role has been highlighted in pathogen-host interactions. Emerging evidence from a variety of virus-host systems indicates that cellular as well as virally encoded miRNAs influence viral replication. Here, we report changes in expression levels of host miRNAs upon ascovirus infection in an insect cell line and investigated the role of a host miRNA, Hz-miR24, in the host-virus system. We found that Hz-miR24 is differentially expressed following virus infection, with an increase in its expression levels late in infection. Experimental evidence demonstrated that Hz-miR24 downregulates ascoviral DNA-dependent RNA polymerase and its β subunit transcript levels late in infection. The specific miRNA-target interactions were investigated and confirmed using the ectopic expression of Hz-miR24 and a green fluorescent protein-based reporter system. Further, the expression of the target gene was substantially enhanced in cells transfected with a synthesized inhibitor of Hz-miR24. These findings suggest that ascoviruses manipulate host miRNAs that in turn regulate the expression of their genes at specific time points after infection. To our knowledge, this is the first cellular miRNA reported to interact with an insect virus.
A Forward Genetic Strategy Reveals Destabilizing Mutations in the Ebolavirus Glycoprotein That Alter Its Protease Dependence during Cell Entry
Authors: Wong Anthony C. , Sandesara Rohini G. , Mulherkar Nirupama , Whelan Sean P. , Chandran Kartik 
Published: October 2009

ABSTRACT
Ebolavirus (EBOV) entry into cells requires proteolytic disassembly of the viral glycoprotein, GP. This proteolytic processing, unusually extensive for an enveloped virus entry protein, is mediated by cysteine cathepsins, a family of endosomal/lysosomal proteases. Previous work has shown that cleavage of GP by cathepsin B (CatB) is specifically required to generate a critical entry intermediate. The functions of this intermediate are not well understood. We used a forward genetic strategy to investigate this CatB-dependent step. Specifically, we generated a replication-competent recombinant vesicular stomatitis virus bearing EBOV GP as its sole entry glycoprotein and used it to select viral mutants resistant to a CatB inhibitor. We obtained mutations at six amino acid positions in GP that independently confer complete resistance. All of the mutations reside at or near the GP1-GP2 intersubunit interface in the membrane-proximal base of the prefusion GP trimer. This region forms a part of the “clamp” that holds the fusion subunit GP2 in its metastable prefusion conformation. Biochemical studies suggest that most of the mutations confer CatB independence not by altering specific cleavage sites in GP but rather by inducing conformational rearrangements in the prefusion GP trimer that dramatically enhance its susceptibility to proteolysis. The remaining mutants did not show the preceding behavior, indicating the existence of multiple mechanisms for acquiring CatB independence during entry. Altogether, our findings suggest that CatB cleavage is required to facilitate the triggering of viral membrane fusion by destabilizing the prefusion conformation of EBOV GP.
African Swine Fever Virus Polyprotein pp62 Is Essential for Viral Core Development
Authors: Suárez Cristina , Salas María L. , Rodríguez Javier M. 
Published: October 2009

ABSTRACT

One of the most characteristic features of African swine fever virus gene expression is its use of two polyproteins, pp220 and pp62, to produce several structural proteins that account for approximately 32% of the total protein virion mass. Equimolecular amounts of these proteins are the major components of the core shell, a thick protein layer that lies beneath the inner envelope, surrounding the viral nucleoid. Polyprotein pp220, which is located immediately underneath the internal envelope, is essential for the encapsidation of the core of the viral particle. In its absence, the infection produces essentially coreless particles. In this study we analyzed, by means of an IPTG (isopropyl-β-
d
-thiogalactopyranoside)-inducible virus, the role of polyprotein pp62 in virus assembly. Polyprotein pp62 is indispensable for viral replication. The repression of polyprotein pp62 expression does not alter late gene expression or the proteolytic processing of the polyprotein pp220. However, it has a profound impact on the subcellular localization of polyprotein pp220. Electron microscopy studies revealed that polyprotein pp62 is necessary for the correct assembly and maturation of the core of the viral particle. Its repression leads to the appearance of a significant fraction of empty particles, to an increase in the number of immature-like particles, and to the accumulation of defective particles. Immunoelectron microscopy analysis showed a clear correlation between the amount of polyprotein pp62, the quantity of polyprotein pp220, and the state of development of the core, suggesting that the complete absence of polyprotein pp62 during morphogenesis would produce a homogenous population of empty particles.
Different Tempo and Anatomic Location of Dual-Tropic and X4 Virus Emergence in a Model of R5 Simian-Human Immunodeficiency Virus Infection
Authors: Ren Wuze , Tasca Silvana , Zhuang Ke , Gettie Agegnehu , Blanchard James , Cheng-Mayer Cecilia 
Published: October 2009

ABSTRACT

We previously reported coreceptor switch in rhesus macaques inoculated intravenously with R5 simian-human immunodeficiency virus SF162P3N (SHIV
SF162P3N
). Whether R5-to-X4 virus evolution occurs in mucosally infected animals and in which anatomic site the switch occurs, however, were not addressed. We herein report a change in coreceptor preference in macaques infected intrarectally with SHIV
SF162P3N
. The switch occurred in infected animals with high levels of virus replication and undetectable antiviral antibody response and required sequence changes in the V3 loop of the gp120 envelope protein. X4 virus emergence was associated with an accelerated drop in peripheral CD4
+
T-cell count but followed rather than preceded the onset of CD4
+
T-cell loss. The conditions, genotypic requirements, and patterns of coreceptor switch in intrarectally infected animals were thus remarkably consistent with those found in macaques infected intravenously. They also overlapped with those reported for humans, suggestive of a common mechanism for coreceptor switch in the two hosts. Furthermore, two independent R5-to-X4 evolutionary pathways were identified in one infected animal, giving rise to dual-tropic and X4 viruses which differed in switch kinetics and tissue localization. The dual-tropic switch event predominated early, and the virus established infection in multiple tissues sites. In contrast, the switch to X4 virus occurred later, initiating and expanding mainly in peripheral lymph nodes. These findings help define R5 SHIV
SF162P3N
infection of rhesus macaques as a model to study the mechanistic basis, dynamics, and sites of HIV-1 coreceptor switch.
Mechanisms of Protein Kinase PKR-Mediated Amplification of Beta Interferon Induction by C Protein-Deficient Measles Virus
Authors: McAllister Christopher S. , Toth Ann M. , Zhang Ping , Devaux Patricia , Cattaneo Roberto , Samuel Charles E. 
Published: October 2009

ABSTRACT

The measles virus P gene products V and C antagonize the host interferon (IFN) response, blocking both IFN signaling and production. Using Moraten vaccine strain-derived measles virus and isogenic mutants deficient for either V or C protein production (V
ko
and C
ko
, respectively), we observed that the C
ko
virus was a potent inducer of IFN-β, while induction by V
ko
virus was an order of magnitude lower than that by the C
ko
virus. The parental recombinant Moraten virus did not significantly induce IFN-β. The enhanced IFN-inducing capacity of the C
ko
virus correlated with an enhanced activation of IFN regulatory factor 3 (IRF-3), NF-κB, and ATF-2 in C
ko
-infected compared to V
ko
or parental virus-infected cells. Furthermore, protein kinase PKR and mitochondrial adapter IPS-1 were required for maximal C
ko
-mediated IFN-β induction, which correlated with the PKR-mediated enhancement of mitogen-activated protein kinase and NF-κB activation. Our results reveal multiple consequences of C protein expression and document an important function for PKR as an enhancer of IFN-β induction during measles virus infection.
Women's rugby league injury claims and costs in New Zealand
Authors: King D A , Hume P A , Milburn P , Gianotti S 
Published: October 2009

AimThere is scarce information on rugby league injuries in female players. This paper provides an overview of the epidemiology of women's rugby league injuries requiring medical treatment and associated costs in New Zealand.MethodNew Zealand Accident Compensation Corporation injury data for the period 1999–2007 were searched for rugby league injury cases occurring in females. Data were analysed by demographics, body region, nature/severity of injury, and medical procedure and costs.ResultsThere were 320 moderate to serious injury claims recorded for females participating in rugby league activities over the study period. There was a mean (SD) of 37.9 (9.5) injury claims per year. The mean cost per year for the study period was $196 514 ($99 133) (£76 066 (£38 374)) with half of the injury claims occurring in New Zealand Maori. Concussion/brain injuries accounted for 3.8% of total female moderate to serious injury claims but accounted for 5.4% of female injury costs ($84 399 (£32 688)) with the highest mean cost per claim ($7033 (£2724)). The lower limb accounted for 65% of the total female injury claims and 58.7% of total injury costs ($922 296 (£356 968)). The mean cost per claim was higher for the lower limb ($4434 (£1714)) than the upper ($3331 (£1288)) limb. Clerks recorded 16.3% of the total injury claims, 20.3% of total injury costs ($319 474 (£123 211)) and had the highest mean cost per claim ($6144 (£2370)). The 25–29 age group recorded 31.9% of injury claims and 33.8% of injury costs. The 35–39 age group recorded the highest mean cost per claim ($6200 (£2392)) but only 10.9% of total claims and 13.8% of total costs.DiscussionWhen compared with other studies in rugby league injuries, it appears that females incur substantially fewer injuries (5.7%) than males (94.3%). Although no participation data by sex are available, it is likely that participation percentages are reflected in the injury percentages. The high frequency (65%) and cost proportion (58.7%) for lower limb injuries was higher in females than in male rugby league players (previously reported as 42.4% of the injury claims and 31.5% of the total injury claim costs for the lower limb).ConclusionsInjury prevention programmes for women's rugby league should focus on the 25–29 age group and address ways to prevent concussion and lower limb injuries.
Physical fitness and indices of lifestyle-related diseases before and after interval walking training in middle-aged and older males and females
Authors: Morikawa M , Okazaki K , Masuki S , Kamijo Y , Yamazaki T , Gen-no H , Nose H 
Published: October 2009

HypothesisWhether increasing peak aerobic capacity for walking (V̇2peak) by interval walking training (IWT) is closely linked with decreasing the indices of lifestyle-related diseases (LSDs) in middle-aged and older people were examined.MethodsFor 4 months from April to September 2005 or 2006, 246 males and 580 females (∼65 years) performed IWT consisting of ≥5 sets of fast walking at ≥70% V̇2peakfor 3 min followed by slow walking at ≤40% V̇2peakfor 3 min ≥4 days/week. Before and after IWT, we measured V̇2peak, body mass index (BMI), %body fat, arterial blood pressure, thigh muscle strength and blood parameters. We analysed 198 males and 468 females who had undergone all the measurements both before and after IWT. To examine the hypothesis, we divided the subjects equally into three groups according to their pretraining V̇2peak: low, middle and high groups for each sex.ResultsBefore training, it was found that thigh muscle strength and blood high-density lipoprotein cholesterol concentration were lower, whereas body weight, BMI, %body fat, arterial blood pressure and blood glucose were higher in the low group than the high group (all, p<0.05). After training, although V̇2peakand thigh muscle strength increased and body weight, BMI, %body fat, blood pressure and blood glucose concentration decreased in all groups (all, p<0.05), the changes were greatest in the low group for both sexes.ConclusionV̇2peakat baseline and changes in response to training were closely linked with indices of LSDs.
Serotonergic mechanisms enhance platelet-mediated thrombogenicity
Authors: Lopez-Vilchez Irene , Diaz-Ricart Maribel , Navalon Fulgencio , Gomez Esther , Gasto Cristobal , Escolar Gines , Galan Ana M. 
Published: October 2009

SummaryAlthough it is generally acknowledged that serotonin (5-HT) is a weak agonist for human platelets, recent information suggests an association between serotonergic mechanisms and cardiovascular risk. We investigated the action of 5-HT on adhesive, cohesive and procoagulant properties of human platelets. Impact of 5-HT on whole blood coagulation and thrombin generation was measured by modified thromboelastometry (TEM) and specific fluorogenic assays. We evaluated the effects of 5-HT on thrombus formation in an in-vitro model of thrombosis using human flowing blood. In platelet-rich plasma (PRP), 5-HT favoured the expression of CD62-P, and procoagulant molecules on platelet membranes. These effects were potentiated in the presence of Ca++ and/or ADP. Incubation with 5-HT accelerated clotting times and augmented clot strength in whole blood TEM, and enhanced thrombin generation in PRP. In perfusion studies, 5-HT significantly increased fibrin deposition at low shear (300s-1) and enhanced platelet thrombus formation on the damaged vascular surface at high shear (1,200s-1). Selective inhibition of serotonin reuptake (SSRI) attenuated effects of 5-HT on platelet activation and downregulated the prothrombotic tendencies observed in the previous experimental conditions. In general, reductions of thrombogenic patterns observed with SSRI were more evident under shear conditions (aggregation and perfusion systems) and less evident under steady conditions (TEM and thrombin generation assays). In conclusion, 5-HT is not a weak agonist for human platelets; instead it accentuates platelet activation, potentiates procoagulant responses on human blood and increases thrombogenesis on damaged vascular surfaces. The remarkable antithrombotic actions achieved through SSRI deserve further mechanistic and clinical investigations.
Vegetation Control in the Long-Term Self-Stabilization of the Liangzhou Oasis of the Upper Shiyang River Watershed of West-Central Gansu, Northwest China
Authors: Bourque Charles P-A. , Hassan Quazi K. 
Published: October 2009

AbstractThis paper explores the relationship between vegetation in the Liangzhou Oasis in the Upper Shiyang River watershed (USRW) of west-central Gansu, China, and within-watershed precipitation, soil water storage, and oasis self-support. Oases along the base of the Qilian Mountains receive a significant portion of their water supply (over 90%) from surface and subsurface flow originating from the Qilian Mountains. Investigation of vegetation control on oasis water conditions in the USRW is based on an application of a process model of soil water hydrology. The model is used to simulate long-term soil water content (SWC) in the Liangzhou Oasis as a function of (i) monthly composites of Moderate Resolution Imaging Spectroradiometer (MODIS) images of land surface and mean air temperature, (ii) spatiotemporal calculations of monthly precipitation and relative humidity generated with the assistance of genetic algorithms (GAs), and (iii) a 80-m-resolution digital elevation model (DEM) of the area. Modeled removal of vegetation is shown to affect within-watershed precipitation and soil water storage by reducing the exchange of water vapor from the land surface to the air, increasing the air’s lifting condensation level by promoting drier air conditions, and causing the high-intensity precipitation band in the Qilian Mountains to weaken and to be displaced upward, leading to an overall reduction of water to the Liangzhou Oasis.
Southeast Pacific Stratocumulus: High-Frequency Variability and Mesoscale Structures over San Félix Island
Authors: Painemal David , Garreaud René , Rutllant José , Zuidema Paquita 
Published: October 2009

Abstract
Stratocumulus cloud cover patterns and their relationship to drizzle were characterized at San Felix Island (SFI; 26.5°S, 80°W) in the southeast Pacific Ocean. Small closed, large closed, and open cells were identified in about 65% of the Moderate Resolution Imaging Spectroradiometer (MODIS) satellite images during 2003. The MODIS imagery was combined with ceilometer and surface meteorological measurements, human observations of cloud types and drizzle, and large-scale meteorological analyses for January through June. The authors identified two drizzle regimes: a synoptically quiescent summer (January–March) regime characterized by a strong anticyclone, large closed cells, and frequent drizzle, and an autumn (April–June) regime characterized by a weaker anticyclone, small closed cells and open cells, and precipitation that was mainly associated with synoptic activity. The large closed cells had higher mean cloud bases and tops than the small closed cells and accounted for 45% of the cumulus-under-stratocumulus reports and 29% of the total drizzle and rain reports. Large closed cells occupied more intermittently coupled boundary layers than did the small closed cells. Open cells also occurred in more decoupled conditions but only accounted for 18% of the total reports of drizzle and rain. The atmospheric stability of large and small closed cells was similar, but large closed cells were more commonly associated with a strong anticyclone, and small closed cells with wave activity superimposed upon a weakened anticyclone. The increased drizzle and occurrence of cumulus-under-stratocumulus in the summer rather than autumn is consistent with higher nighttime liquid water paths. A contribution of this study is the documentation of the ways in which synoptic activity can affect stratocumulus decks.
A Technique to Censor Biological Echoes in Radar Reflectivity Data
Authors: Lakshmanan Valliappa , Zhang Jian , Howard Kenneth 
Published: October 2009

AbstractExisting techniques of quality control of radar reflectivity data rely on local texture and vertical profiles to discriminate between precipitating echoes and nonprecipitating echoes. Nonprecipitating echoes may be due to artifacts such as anomalous propagation, ground clutter, electronic interference, sun strobe, and biological contaminants (i.e., birds, bats, and insects). The local texture of reflectivity fields suffices to remove most artifacts, except for biological echoes. Biological echoes, also called “bloom” echoes because of their circular shape and expanding size during the nighttime, have proven difficult to remove, especially in peak migration seasons of various biological species, because they can have local and vertical characteristics that are similar to those of stratiform rain or snow. In this paper, a technique is described that identifies candidate bloom echoes based on the range variance of reflectivity in areas of bloom and uses the global, rather than local, characteristic of the echo to discriminate between bloom and rain. Every range gate is assigned a probability that it corresponds to bloom using morphological (shape based) operations, and a neural network is trained using this probability as one of the input features. It is demonstrated that this technique is capable of identifying and removing echoes due to biological targets and other types of artifacts while retaining echoes that correspond to precipitation.
Global Circuit Model with Clouds
Authors: Zhou Limin , Tinsley Brian A. 
Published: October 2009

AbstractCloud data from the International Satellite Cloud Climatology Project (ISCCP) database have been introduced into the global circuit model developed by Tinsley and Zhou. Using the cloud-top pressure data and cloud type information, the authors have estimated the cloud thickness for each type of cloud. A treatment of the ion pair concentration in the cloud layer that depends on the radii and concentration of the cloud droplets is used to evaluate the reduction of conductivity in the cloud layer. The conductivities within typical clouds are found to be in the range of 2%–5% of that of cloud-free air at the same altitude, for the range of altitudes for typical low clouds to typical high clouds. The global circuit model was used to determine the increase in columnar resistance of each grid element location for various months in years of high and low volcanic and solar activity, taking into account the observed fractional cloud cover for different cloud types and thickness in each location. For a single 5° × 5° grid element in the Indian Ocean, for example, with the observed fractional cloud cover amounts for low, middle, and high clouds each near 20%, the ionosphere-to-surface column resistance increased by about 10%. (For 100%, fraction—that is, uniformly overcast conditions—for each of the cloud types, the increase depends on the cloud height and thickness and is about a factor of 10 for each of the lower-level clouds in this example and a factor of 2 for the cirrus cloud.) It was found that treating clouds, in the fraction of each grid element in which they were present, as having zero conductivity made very little difference to the results. The increase in global total resistance for the global ensemble of columns in the ionosphere–earth return path in the global circuit was about 10%, applicable to the several solar and volcanic activity conditions, but this is probably an upper limit, in light of the unavailability of data on subkilometer breaks in cloud cover.