bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitigating early phosphatidylserine exposure in a Tmem30a dependent way ameliorates neuronal damages after ischemic stroke
Authors: Wu Di , Wu Chuanjie , Duan Yunxia , Guo Jiaqi , He Jiachen , Xu Shuaili , Liu Guiyou , Zhou Chen , Ding Yuchuan , Zhu Xianjun , Ji Xunming 
Published: May 2024

Abstract

Externalized phosphatidylserine (PS) on cell membranes is well-known as a late stage “eat me” signal for dead cells that is routinely used as standard marker of apoptotic cell death. Here, we show that unexpectedly, externalized PS is not just a marker of dead cells but may play an active role in regulating early phases of neuronal death in stroke. We found that transmembrane protein 30A (Tmem30a), the β-subunit of P4-ATPases, were increased in the penumbra in two types of stroke models. Tmem30a upregulation was associated with less PS exposure and neurological benefits in models of ischemia/reperfusion, but not in permanent ischemia. Furthermore, treatment with recombinant Annexin V, having a high affinity with phosphatidylserine, decreased PS exposure and mitigated neurological impairments in wild-type mice after ischemia/reperfusion, but not in Tmem30a knockout mice. Mechanistically, mitigation of PS exposure, medicated by Tmem30a/Annexin V, led to decreased expression of apoptosis and necroptosis markers in neurons of peri-infarct areas and led to improved neurologic outcomes. In a proof-of-concept analysis of acute stroke patients, increased plasma Annexin V were associated with early neurological improvement in patients with salvageable penumbra after endovascular treatment. Taken together, these findings suggest that Tmem30a-mediated externalization of PS may be a novel mechanism and target in ischemic stroke.
Diagnosis and Management of Inferior Alveolar Nerve Injury Caused by Calcium Hydroxide Extrusion during Root Canal Treatment: Case Report
Authors: Almaslamani Manal , Alhassawe Suliman , Prasad Prathibha , Alshayeb Maher 
Published: May 2024

Abstract

This report highlights a rare occurrence of inferior alveolar nerve injury resulting from calcium hydroxide extrusion during endodontic treatment. The patient experienced severe pain and paraesthesia in the lower right lip following root canal treatment due to inadvertent overflow of calcium hydroxide into the inferior alveolar nerve canal. No surgical intervention was performed, the patient received non-steroidal anti-inflammatory analgesics and Vitamin B complex injections to manage the symptoms. At six months, complete resorption of the root canal material occurred, resulting in further reduction in paraesthesia. Two-year follow-up, complete disappearance of paraesthesia occurred. The importance of the preoperative radiographs is highlighted. The lack of radiographic assessment at the patient's request led to calcium hydroxide overflow.
Agrochemical Oryzemate treatment controls the uptake of hydrophobic organic pollutants through the regulation of major latex-like proteins
Authors: Chitose Natsumi , Fujita Kentaro , Chujo Maho , Inui Hideyuki 
Published: May 2024

Abstract

The Cucurbitaceae family accumulates hydrophobic organic pollutants in their aerial parts at high concentrations. Major latex-like proteins (MLPs) identified in Cucurbita pepo bind to hydrophobic organic pollutants in roots, forming complexes that are transported via xylem vessels to the plant’s aboveground parts. However, soil remediation of hydrophobic organic pollutants utilizing MLPs has not been established. In this study, the uptake of hydrophobic organic pollutants was promoted by C. pepo through the upregulation of MLP genes by agrochemical treatment. It was found that probenazole, an active ingredient in the agrochemical Oryzemate, upregulated the promoter activity of MLP genes in the roots of transgenic tobacco plants. Oryzemate treatment increased the amount of MLPs in the roots and xylem sap of C. pepo. Oryzemate treatment slightly increased and significantly decreased the concentration of the hydrophobic organic pollutant pyrene in the xylem sap of C. pepo cultivated in high- and low-contamination soils, respectively. Probenazole competitively inhibits the binding of MLPs to pyrene, thereby suppressing its uptake by C. pepo in low-contamination soil. This study demonstrates that Oryzemate possesses dual effects: effective phytoremediation and safe crop production, depending on the soil contamination level.
Pathological and molecular insights into intravenous leiomyomatosis: an integrative multi-omics study
Authors: Yin Sheng , Shi Peipei , Han Jing , Li Hua , Ren Aimin , Ma Li , Tang Wenbin , Liu Wenxue , Yu Sihui , Li Tingting , Wang Chunsheng , Hou Yingyong , Zhang Jiarong 
Published: May 2024

Abstract

Intravenous leiomyomatosis (IVL) is a histologically well differentiated smooth muscle tumor with aggressive behavior, capable of extending throughout the venous system. Due to its rarity, systematic studies on IVL are limited. We conducted a comprehensive multi-omics study, collecting tissues from IVL, uterine fibroid, and normal myometrium. Single-cell RNA sequencing analysis revealed a significant difference in cell composition between IVL and uterine fibroid. H&E staining demonstrated more frequent hydropic change and hyalinization, with decreased vascular density in IVL tissues compared to both normal myometrium and uterine fibroid. Proteomics analysis in 8 paired IVL and normal myometrium fresh frozen tissue identified differentially expressed proteins mainly enriched in focal adhesions and regulation of the actin cytoskeleton. The most frequently involved chromosomes included deletions in 10q22.2, 10q24.32, 13q14, and 13q21-31. Correlation analyses highlighted chromosome 10q as the most frequent cytoband, with corresponding proteins involved in regulating focal adhesions and the cytoskeleton. Integrated analysis between pathological and clinical characteristics indicated that chromosome 10q deletion and vascular morphology in IVL could serve as important markers predicting aggressive behavior. Our study illuminates the pathological and molecular changes associated with IVL, offering insights that may contribute to establishing new directions for IVL treatment.
Enhancing community awareness of antimicrobial use and resistance through Community Conversations in rural Ethiopia
Authors: Lemma Mamusha , Alemu Biruk , Amenu Kebede , Wieland Barbara , Knight-Jones Theodore 
Published: May 2024

Abstract

Background
Enhanced availability of veterinary drugs in Ethiopia has led to a growing use of these drugs in food-producing animals. However, the use of drugs in livestock production is not commonly supervised. In addition, smallholder livestock keepers often lack sufficient training and information about how the drugs work and their appropriate usage to achieve the intended impact.
Methods
We used Community Conversations to raise awareness and knowledge about antimicrobial use among rural communities. This revealed perceptions and practices of rural households regarding the use of antimicrobials and the risk of drug resistance in livestock. We used women-only, men-only, and mixed-gender discussion groups to explore the gendered perceptions and practices of community members regarding antimicrobial use and resistance.
Results
The discussions with rural communities identified factors that contribute to the misuse of antimicrobials, including poor regulatory system, limited diagnostic capacity, and inadequate knowledge and information among community members regarding the appropriate usage of antimicrobials and the potential resistance emergence.
Conclusions
Raising awareness about antimicrobial use and the potential emergence of resistance is an effective approach to promoting behavior change among rural communities concerning the use of antimicrobials and enhancing livestock management practices. The insights gained from understanding the perceptions and practices of rural communities regarding the use of antimicrobials can help in developing targeted education and training programs for stakeholders involved in the antimicrobial supply chain.
Exploring magma composition variability at various tectonic settings, using global volcanic rock composition data with the DashVolcano tool
Authors: Oggier Frederique , Widiwijayanti Christina , Costa Fidel 
Published: May 2024

Abstract

The relationships between plate tectonics, volcanoes, and the magma compositions their erupt are very important for understanding the whole-earth geodynamics. To understand how these first order phenomena are related requires the integration of global datasets from different sources and tools that would allow to visualize and access the data in an efficient manner, which is a challenging task. Here we report a new version of the application tool called DashVolcano which has linked the global record of all Holocene volcanoes from the Global Volcanism Program with the major element composition of erupted rocks from GEOROC and PetDB databases (~ 140,000 rock samples in total) according to tectonic settings. The tool allows to visualize the rock compositions at a global scale with a map view, and to plot their compositions using classical classification diagrams (TAS, AFM) for individual volcanoes or for a region of interest. Moreover, radar plots permit the visualization of the relative abundance of rock types per volcano or per region of interest and how they compare to various tectonic settings. The DashVolcano application allows to explore a range of questions related to the abundance of erupted magma types in different tectonic settings, the differences in rock composition within or between a chosen region(s), volcano(es), and tectonic settings. DashVolcano should be a useful tool for research and teaching, and it is a good example of the challenges and opportunities of linking large databases at a global scale. DashVolcano is publicly available at https://dashvolcano-qpmiyp54bq-as.a.run.app/
Revolutionizing Corneal Staining Assessment: Advanced Evaluation through Lesion-aware Fine-Grained Knowledge Distillation
Authors: Yuan Jin , Deng Yuqing , Cheng Pujin , Xu Ruiwen , Ling Lirong , Xue Hongliang , Zhou Shiyou , Huang Yansong , Lyu Junyan , Wang Zhonghua , Wong Kenneth , Zhang Yimin , Yu Kang , Zhang Tingting , Hu Xiaoqing , Li Xiaoyi , Lou Yan , Tang Xiaoying 
Published: May 2024

Abstract

Corneal staining is crucial for evaluating ocular surface diseases, yet existing AI models for CSS (Corneal Staining Score) assessments struggle with detailed lesion identification and lack applicability in real-world clinical settings. Moreover, the output of current AI-assist staining evaluation system only provides categories of grades, leading to potential “plateau” effect, which could misrepresent treatment response in clinical practices. Addressing these gaps, we developed the Fine-grained Knowledge Distillation Corneal Staining Score (FKD-CSS) model, which effectively distills fine-grained features into the CSS grading process and outputs continuous, nuanced scores for thorough assessments. Trained on 1471 images from 14 centers of heterogenous sources, FKD-CSS demonstrates robust accuracy with a Pearson's r of 0.898 against ground-truth and an area under the curve (AUC) of 0.881 in internal validation, rivaling senior ophthalmologists. Additionally, the model achieved expert performance with considerable Pearson's r (0.844–0.899) and AUCs (0.804–0.883) in external tests in six regions of China using 2376 corneal staining images of dry eye across 23 hospitals, and generalizes to multi-ocular-surface-disease test (Pearson's r: 0.816, AUC: 0.807), underscore its efficiency and explainability for CSS assessment. These results highlight FKD-CSS's potential as a precise, valuable tool for staging and outcome measurement of ocular surface diseases.
Enhancing the Productivity and Assembly Line Balancing Through Takt Time Implementation
Authors: Khalil Muhammad , Din Muhammad Shihab Ud , Ali Abdul Basit , Tahir Muhammad 
Published: May 2024

Abstract

In Pakistan, automotive sector is setting leaps in term of expansion, and achieved a phenomenal growth of 171% between the years 2014 and 2018. Keeping in view, the high demand and production of the sector, it is crucial to generate fruitful revenue from it. For this purpose, the production lines efficiency and productivity should be significantly optimized. Nowadays majority of industries are shifting towards green manufacturing with the application of lean manufacturing tools such as implementation of Takt Time. The proposed study is carried out at Honda Pakistan to reduce the cycle time of KIRA machine through line balancing techniques (Largest Candidate Rule, Longest-Task-Time Heuristic and Mathematical Modelling), capacity calculation, and validated the results via Rockwell Arena simulation software. Moreover, cost benefit analysis is committed to check economic effect of proposed model. Further, after implementation of proposed model, the cycle time of KIRA is reduced from 68.8 sec to 59.08 sec (takt time), and production rate is enhanced from 2800 parts to 3263 parts per day. Lastly, bottleneck is resolved, ultimately leading to increase in line efficiency and station utilization, while on other hand balance delay and line smoothing index are decreased.
Development and validation of the Educational Psychology Workplace Motivation (EPWM) scale; A measure to evaluate educational psychologists’ working-life contexts, patterns, and motivation for change.
Authors: Sewell Alexandra , Coley Fiona , Gossman Peter , Park Jane 
Published: May 2024

Abstract

This research builds on recent interest in Educational Psychologists’ (EPs) working-life contexts, patterns, and motivations. It seeks to deepen the knowledge made through Department for Education research (DfE, 2023) by presenting the development and validation of the Educational Psychology Workplace Motivation (EPWM) scale. The paper reports the application of the theory of motivational interviewing to the construction of the EPWM scale. The measure development process, including content validity and construct validity, is described, highlighting the rigorous validation procedures adopted. Suggestions for further strengthening the measure's rigor, such as establishing criterion validity through comparison with other measures, are discussed. Additionally, the analysis of data collected from 201 practicing EPs provides further insights into work-life contexts and patterns. The findings suggest that EPs often work beyond their contracted hours, have high caseloads, and produce a significant number of reports each academic year. The data also reveal a tendency for more time to be spent on school visits compared to home visits, shedding light on the locations of EPs' direct contact work. By making the EPWM scale available as an open-access resource, the study encourages further development and adaptation by the EP research community. In relation to recent renewed interest in EP working life experiences and motivations (DfE, 2023), the validation of the measure serves as a complementary step towards understanding EPs' work-life experiences and aims to stimulate further research in this area.
Integrative Analysis of miRNA Expression Profiles Reveals Distinct and Common Molecular Mechanisms Underlying Broad Diagnostic Groups of Severe Mental Disorders
Authors: Poschmann Jeremie , Solomon Pierre , Kaurani Lalit , Budde Monika , Guiné Jean-Baptiste , Krüger Dennis , Centeno Tonatiuh , Burkhardt Susanne , Fourgeux Cynthia , Adorjan Kristina , Heilbronner Maria , Kalman Janos , Kohshour Mojtaba Oraki , Papiol Sergi , Reich-Erkelenz Daniela , Schaupp Sabrina , Schulte Eva , Senner Fanny , Vogl Thomas , Anghelescu Ion-George , Arolt Volker , Baune Bernhard , Dannlowski Udo , Dietrich Detlef , Fallgatter Andreas , Figge Christian , Juckel Georg , Konrad Carsten , Reimer Jens , Reininghaus Eva , Schmauß Max , Spitzer Carsten , Wiltfang Jens , Zimmermann Jörg , Schütz Anna-Lena , Sananbenesi Farahnaz , Sauvaget Anne , Falkai Peter , Schulze Thomas , Fisher Andre , Heilbronner Urs 
Published: May 2024

Abstract

Micro RNAs (miRNAs) play a crucial role as regulators of various biological processes and have been implicated in the pathogenesis of mental disorders such as schizophrenia and bipolar disorders. In this study, we investigate the expression patterns of miRNAs in the PsyCourse Study (n=1,786), contrasting three broad diagnostic groups: Psychotic (Schizophrenia-spectrum disorders), Affective (Bipolar Disorder I, II and recurrent Depression), and neurotypic healthy individuals. Through comprehensive analyses, including differential miRNA expression, miRNA transcriptome-wide association study (TWAS), and predictive modelling, we identified multiple miRNAs unique to Psychotic and Affective groups as well as shared by both. Furthermore, we performed integrative analysis to identify the target genes of the dysregulated miRNAs and elucidate their potential roles in psychosis. Our findings reveal significant alterations of multiple miRNAs such as miR-584-3p and miR-99b-5p across the studied diagnostic groups, highlighting their role as molecular correlates. Additionally, the miRNA TWAS analysis discovered previously known and novel genetically dysregulated miRNAs confirming the relevance in the etiology of the diagnostic groups. Importantly, novel factors and putative molecular mechanisms underlying these groups were uncovered through the integration of miRNA-target gene interactions. This comprehensive investigation provides valuable insights into the molecular underpinnings of severe mental disorders, shedding light on the complex regulatory networks involving miRNAs.
ZFTA fusion oncoproteins drive tumorigenesis through transcriptional condensates
Authors: Arabzade Amir , Shirnekhi Hazheen , Varadharajan Srinidhi , Ippagunta Siri , Laboe Nicolas , Gee David , Bhimsaria Devesh , Emanus Erik , Kardian Alisha , Phillips Aaron , Cambell George , Park Cheong-Gil , Freyaldenhoven Toler , Chandra Bappaditya , Xia Youlin , Bonten Erik , Achari Anushree , Kandikonda Suresh , Carisey Alexandre , Deneen Benjamin , Bertrand Kelsey , Tripathi Swarnendu , Shirinifard Abbas , Petersen Becky , King Sharon , Stabley Daniel , Pitre Aaron , Kriwacki Richard , Mack Stephen 
Published: May 2024

Abstract

Over 95% of EPNs that arise in the cortex are driven by a gene fusion involving the zinc finger translocation associated (ZFTA) protein. Using super-resolution and lattice light sheet microscopy, we demonstrate that the most frequent fusion variant, ZFTA-RELA(ZR), forms dynamic nuclear condensates that are required for oncogene expression and tumorigenesis. Mutagenesis of ZR reveals key intrinsically disordered regions (IDRs) that govern condensate formation. Condensate-modulating ZR IDR mutations impaired genomic occupancy at oncogenic loci, and inhibited the recruitment of transcriptional effector proteins, such as MED1, BRD4 and RNA polymerase II. Using nuclear magnetic resonance spectroscopy, we resolved the protein structure of the critical zinc finger found in ZR, and characterized its significance for condensate formation, genomic binding, and oncogene activation. Our data leverages microscopy, genomics, cell biology, animal modeling, structural biology, and machine learning approaches to provide mechanistic insights into the processes that govern oncogene expression in ZFTA fusion-driven tumors.
Relation Cross-fusion Attention Assistance Networks for Multi-hop Question Answering over Knowledge Graphs
Authors: Lv Yana , Bao Haomiao , Du Xiuli , Qiu Shaoming 
Published: May 2024

Abstract

Multi-hop knowledge graph question answering aims to find answer entities from the knowledge graph based on natural language questions. This is a challenging task as it requires precise reasoning about entity relationships at each step. When humans perform multi-hop reasoning, they usually focus on specific relations between different hops and determine the next entity. However, most algorithms often choose the wrong specific relations, causing the system to deviate from the correct reasoning path. In multi-hop question answering, the specific relation between each hop is crucial. The existing TransferNet model mainly relies on question representation for relational reasoning, but cannot accurately calculate the specific relational distribution, which will profoundly affect question answering performance. On this basis, this paper proposes an interpretable assiatance framework, which makes full use of relation embedding and question semantics, and uses the attention mechanism to cross-fuse the relevant information of them to assist in calculating the relation distribution of each hop. Extensive experiments are conducted on two English datasets, WebQSP and CWQ, demonstrating that the proposed model outperforms state-of-the-art models by a large margin.
Transcriptome and metabolome conjoint analysis revealed that PaGLK affects photosynthesis and composition of root exudates in poplar
Authors: Bai Xiang-dong , Zheng Yu , Cao Li , Wang Wei , Jiang Jing , Yu Qi-bin , Yang Chuan-ping , Liu Gui-feng 
Published: May 2024

Abstract

Background
Photosynthetic carbon fixation is the main source of root exudates. GOLDEN2-LIKE (GLK) genes play an important role in photosynthetic carbon fixation. Previous studies have found that expression-inhibited the PaGLK in poplar reduce its net photosynthesis. However, the relationship between GLK genes, root exudates and photosynthetic carbon fixation and how photosynthesis affects root exudate in poplar are not clear.
Result
In this study, we performed comparative transcriptome and metabolome analyses of overexpression and suppression transgenic poplar. GO enrichment analysis showed that the downregulation of differentially expressed genes (DEGs) in suppression lines was mainly related to photosynthesis in biological processes. Specifically, photosynthesis-antenna proteins, porphyrin and chlorophyll metabolism, and photosynthesis were significantly enriched in KEGG pathways. Gene expression showed consistent trends in real time quantitative PCR (RT-qPCR) and transcriptome, indicating reliable transcriptome. Differentially expressed metabolites (DEMs) of root exudates were mainly enriched in amino acid metabolism, glucose metabolism and fatty acid metabolism pathways. After correlating DEGs and DEMs, we found that most genes and metabolites showed positive regulation.
Conclusion
This study shows that the new factors change composition of root exudates.