bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of the complete mitochondrial genome of an endemic species in China, Aulocera merlina (Lepidoptera: Nymphalidae: Satyrinae) and phylogenetic analysis within Satyrinae
Authors: Shi Qinghui , Xie Jinling , Wu Jialing , Chen Shengchung , Sun Gang , Zhang Juncheng 
Published: May 2024

AbstractThe mitochondrial genome (mitogenome) has been extensively used as molecular markers in determining the insect phylogenetic relationships. In order to resolve the relationships among tribes and subtribes of Satyrinae at the mitochondrial genomic level, we obtained the complete mitogenome of Aulocera merlina (Oberthür, 1890) (Lepidoptera: Nymphalidae: Satyrinae) with a size of 15,259 bp. The mitogenome consisted of 37 typical genes, including 13 protein‐coding genes (PCGs), 2 ribosomal RNA genes (rRNAs), 22 transfer RNA genes (tRNAs), and an A + T‐rich region. The gene organization and arrangement were similar to those of all other known Satyrinae mitogenomes. All PCGs were initiated with the canonical codon pattern ATN, except for the cox1 gene, which used an atypical CGA codon. Nine PCGs used the complete stop codon TAA, while the remaining PCGs (cox1, cox2, nad4, and nad5) were terminated with a single T nucleotide. The canonical cloverleaf secondary structures were found in all tRNAs, except for trnS1 which lacked a dihydrouridine arm. The 448 bp A + T‐rich region was located between rrnS and trnM, and it included the motif ATAGA followed by a 19‐bp poly‐T stretch and a microsatellite‐like (TA)6 element preceded by the ATTTA motif. The phylogenetic tree, inferred using Bayesian inference and maximum likelihood methods, generated similar tree topologies, revealing well‐supported monophyletic groups at the tribe level and recovering the relationship ((Satyrini + Melanitini) + ((Amathusiini + Elymniini) + Zetherini)). The close relationship between Satyrina and Melanargiina within the Satyrini was widely accepted. Additionally, Lethina, Parargina, and Mycalesina were closely related and collectively formed a sister group to Coenonymphina. Moreover, A. merlina was closely related to Oeneis buddha within the Satyrina. These findings will provide valuable information for future studies aiming to elucidate the phylogenetic relationships of Satyrinae.
Strength and conditioning in dance: A systematic review and meta‐analysis
Authors: Ngo Jake K. , Lu Jie , Cloak Ross , Wong Del P. , Devonport Tracey , Wyon Matthew A. 
Published: May 2024

AbstractTo assess the evidence for the effect of strength and conditioning on physical qualities and aesthetic competence in dance populations, three electronic databases (PubMed, Scopus, SPORTDiscus) were searched (until September 2022) for studies that met the following criteria: (i) dancers aged >16 years; (ii) structured strength and conditioning intervention; and (iii) with physical qualities and aesthetic competence as outcome measures. Methodological quality and risk of bias of the included studies were assessed through the systematic review tool “QualSyst”. Meta‐analyses of effect sizes (Hedges'g) with forest plots explored the effects of the strength and conditioning interventions. Thirty‐six studies met the inclusion criteria and were included in this review. Meta‐analysis indicated strength and conditioning significantly (p < 0.05) improved lower body power (g = 0.90, 95% CI: 0.53–1.27), upper body strength (g = 0.98, 95% CI: 0.39–1.57), lower body strength (g = 1.59, 95% CI: 0.97–2.22), and flexibility (g = 0.86, 95% CI: 0.05–1.66). Strength and conditioning interventions were found to be effective at improving physical qualities in dancers, recommending their participation in additional sessions to enhance overall fitness and ultimately dance performance. It is recommended that future strength and conditioning intervention research should include sample size calculations, with participants recruited from a specific dance genre and skill level in order to evaluate how strength and conditioning influences dance performance.
Association between dietary intake of creatine and female reproductive health: Evidence from NHANES 2017–2020
Authors: Ostojic Sergej M. , Stea Tonje Holte , Ellery Stacey J. , Smith‐Ryan Abbie E. 
Published: May 2024

AbstractThe hormonal changes in women influence creatine dynamics, emphasizing its potential importance during menstruation, pregnancy, postpartum, menopause, and postmenopause. Yet, limited research explores creatine's impact on female reproductive health at the population level. Our study investigated the relationship between dietary creatine intake and reproductive health indices in US women using data from the 2017–2020 National Health and Nutrition Examination Survey (NHANES). We extracted a dataset containing females aged 12 years and above who provided details about their reproductive health and dietary habits. Daily creatine intake was quantified as a relative amount (mg per kg body mass) and did not include creatine from dietary supplements and pharmacological agents. A daily requirement for dietary creatine for healthy women was employed to classify respondents into two separate subpopulations: (1) suboptimal intake of creatine (<13 mg per kg body mass per day) or (2) recommended intake (dietary creatine ≥ 13 mg per kg body mass per day). A total of 4522 female participants from the NHANES study (age 44.5 ± 20.5 years) provided data on their reproductive health and dietary intake. The average daily creatine intake for the group was 10.5 ± 10.8 mg per kg body mass. The odds ratio for having irregular periods in women consuming ≥13 mg of creatine per kg body mass daily (recommended intake) compared to those with suboptimal intake was 0.75 (95% CI, from 0.66 to 0.86), indicating a significant association between higher intake of dietary creatine and lower risk of oligomenorrhea (p < .001). Moreover, women consuming less than 13 mg of creatine per kg body mass faced an increased risk of fetal macrosomia (OR 1.26; p = .04), pelvic infection (OR 1.68; p = .01), hysterectomy (OR 1.42; p < .001), oophorectomy (OR 1.54; p < .001), and receiving hormone replacement therapy (OR 1.26; p = .02). Consuming a creatine‐rich diet has been linked to lower risks of reproductive issues in US women aged 12 and above. Those consuming ≥13 mg of creatine per kg body mass daily showed notably lower risks of irregular menstrual periods, obstetric conditions, and pelvic pathology. Further studies are needed to confirm these potential benefits.
Early risk for child externalising symptoms: Examining genetic, prenatal, temperamental and parental influences
Authors: Lee Sohee , Robertson Olivia C. , Marceau Kristine , Knopik Valerie S. , Natsuaki Misaki N. , Shaw Daniel S. , Leve Leslie D. , Ganiban Jody M. , Neiderhiser Jenae M. 
Published: May 2024

AbstractThis study utilised the Early Growth and Development Study (N = 561 adoptive children; 57.2% male, 55.3% White), a study of children adopted at birth, to examine heritable (birth parent psychopathology) and prenatal risk (prenatal maternal distress and smoking during pregnancy), infant negative affectivity, adoptive parent over‐reactivity and warmth as independent predictors of childhood externalising symptoms. The current study evaluated if: (1) infant negative affectivity and over‐reactive parenting are candidate mediators for the effects of heritable and prenatal risk on externalising symptoms and (2) parental warmth weakens the influence of heritable risk, prenatal risk, negative affectivity and over‐reactive parenting on externalising symptoms. There were main effects of heritable risk, infant negative affectivity and over‐reactive parenting on child externalising symptoms. The study found no support for the hypothesised mediation and moderation effects, suggesting that targeting parental over‐reactivity rather than warmth would be more effective in reducing the risk for childhood externalising symptoms.
Nutrient recovery from manure digestate by using waste magnesite powder and bone meal as sustainable substitutes for struvite precipitation
Authors: Kutlar Feride Ece , Engin Iskin , Yilmazel Yasemin Dilsad 
Published: May 2024

AbstractBACKGROUNDStruvite (MgNH4PO4.6H2O) precipitation is a sustainable approach that can offer simultaneous removal/recovery of nutrients from biogas plant effluents. However, most biogas plant effluents contain a higher molar concentration of nitrogen (N) than magnesium (Mg) and phosphorus (P). Therefore, the external addition of Mg2+ and phosphate (PO43‐) salts is needed to maximize the recovery. In this study, Mg‐rich waste magnesite powder and P‐rich bone meal were used as sustainable additives. The Box–Behnken design was applied to determine optimum conditions of process parameters (pH, Mg:N and P:N molar ratios) to maximize ammonium‐nitrogen (NH4‐N), PO4‐P and Mg2+recoveries.RESULTSNH4‐N, PO4‐P and Mg2+ recoveries were in the ranges 78.5%–96.9%, 69.2%–96.3% and 75.1%–99.9%, respectively. The actual and predicted values showed significant consistency, indicating that the model is satisfactory. Under optimum conditions (pH = 9.0, Mg:N = 2.2, P:N = 1.8), 97.8 ± 0.1% NH4‐N, 96.6 ± 0.31% PO4‐P and 84.4 ± 0.9% Mg2+ recovery were attained. The X‐ray diffraction results confirmed the sole presence of struvite crystals. Scanning electron microscopy images showed irregular prismatic orthorhombic crystals and amorphous material depositions on precipitates of ~25 μm crystal size. The product was 49% struvite with 15.2% P content. The heavy metal content was lower than regulatory limits.CONCLUSIONEven though waste material and industrial by‐products have been used as additives in this process, high NH4‐N and PO4‐P recoveries were recorded under optimum conditions. These results are promising and illustrate a powerful example of industrial symbiosis, and the precipitate containing struvite can serve as a valuable product. © 2024 The Authors. Journal of Chemical Technology and Biotechnology published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry (SCI).
Miniaturized gas exposure devices for atom probe experiments
Authors: Ott Benedict , Heller Martina , Monajem Mehrpad , Felfer Peter 
Published: May 2024

AbstractTo detect hydrogen in materials at the atomic scale, atom probe tomography is now regularly used. In order to avoid cumbersome cryo‐preparation to suppress diffusion, often hydrogen is charged only into the finished specimen. Here, the use of hydrogen gas over electrochemical hydrogen has the advantage that the specimen is not contaminated with an electrolyte. So far, this “charging” has been done in large, expensive systems. Here, we introduce small devices that enable the exposure of atom probe specimens to hydrogen and potentially other gases, using only very small gas volumes. This enables the operation in regular laboratory environments without additional safety measures. These devices can be used to expose the specimen to hydrogen up to 10 bar/90°C. Higher temperatures may be attained with small changes. Validation of the success of charging with these setups is demonstrated through experiments employing deuterium charging of palladium atom probe tips.Research Highlights
Enabling the exposure of atom probe specimens to hydrogen and potentially other gases, using only very small gas volumes.
Built setups can be assembled with little money and from freely available parts, making specimen charging much easier.
Validation of the success of charging with these setups is demonstrated through experiments employing deuterium charging of palladium atom probe tips.
Belantamab mafodotin in triple‐refractory multiple myeloma patients: A retro‐prospective observational study in Italy
Authors: Fazio Francesca , Petrucci Maria Teresa , Corvatta Laura , Piciocchi Alfonso , Pepa Roberta Della , Tacchetti Paola , Musso Maurizio , Zambello Renato , Belotti Angelo , Bringhen Sara , Antonioli Elisabetta , Conticello Concetta , Renzo Nicola Di , De Stefano Valerio , Musto Pellegrino , Gamberi Barbara , Derudas Daniele , Boccadoro Mario , Offidani Massimo , Morè Sonia 
Published: May 2024

AbstractBelantamab mafodotin is the first‐in‐class antibody‐drug conjugates targeting B‐cell maturation antigen to have demonstrated effectiveness in triple‐class refractory multiple myeloma (TCR‐MM) patients. We performed a retrospective study including 78 TCR patients, with at least four prior lines of therapy (LOTs), who received belantamab mafodotin within named patient program and expanded access program in Italy between 2020 and 2022. Median age was 65 years (range 42–86 years), ECOG performance status was ≥1 in 45% of patients. Overall, a clinical benefit was obtained in 36 out of 74 evaluable patients (49%), with 43%, 28%, and 13.5% achieving at least partial response, very good partial response, and complete response, respectively. After a median follow‐up of 12 months (range 6–21 months), median duration of response, progression‐free survival (PFS), and overall survival (OS) were 14, 5.5, and 12 months, respectively. Age >70 years, good performance status and response were associated with longer PFS and OS. Keratopathy occurred in 58% of patients (G3 2.5%), corneal symptoms in 32% (G3 1.2%) and a reduction in visual acuity in 14%. Grade 3 thrombocytopenia occurred in 9% of patients. Only 3% of patients discontinued belantamab mafodotin because of side effects. This real‐life study demonstrated significant and durable responses of belantamab in TCR‐MM patients with four prior LOTs, otherwise ineligible for novel immunotherapies.
Evaluation of the prognostic impact of pathologic tumor regression grade on patients with colorectal cancer after preoperative chemoradiotherapy
Authors: Nakao Eiichi , Honda Michitaka , Uesugi Noriyuki , Osakabe Mitsumasa , Sato Ayaka , Todate Yukitoshi , Yaegashi Mizunori , Takano Yoshinao , Sasaki Akira , Kono Koji , Sugai Tamotsu 
Published: May 2024

AbstractBackgroundThe prognostic value of the pathological response to preoperative chemoradiotherapy (CRT) in rectal cancer (RC) remains unknown.ObjectivesWe aimed to assess the predictive value of the response to CRT that was derived from an evaluation of the histological findings (whole‐section vs. representative‐section sampling) and attempted to determine an objective cut‐off value for the tumor regression grade (TRG).MethodsWe examined the association of the TRG with the outcomes (recurrence‐free survival [RFS] and overall survival [OS]) of 78 patients with RC. Patients with RC treated with preoperative CRT were divided into development (30 cases) and validation (48 cases) cohorts. The TRG was classified as grades I (Ia, Ib), II, and III. The cut‐off value was determined by receiver operating characteristic (ROC) curve analysis.ResultsThe TRG determined from whole‐section sampling versus representative‐section sampling was more strongly correlated with patient survival. We found that in both cohorts, patients with a cut‐off value of <73% had a poor prognosis. Finally, the cut‐off value was found to be an independent predictive factor in both univariate and multivariate analysis.ConclusionsThe TRG that was used to evaluate patients with RC who underwent preoperative CRT was an independent prognostic factor for outcome.
Decreased femoral trochlea axial orientation corrected by derotational distal femur osteotomy in patients with patellar dislocation yields satisfactory outcomes
Authors: Li Kehan , Chen Xiaobo , Huo Zhenhui , Kang Huijun , Wang Boyu , Wang Fei 
Published: May 2024

AbstractPurposeThe femoral trochlea axial orientation has been shown to be a better predictor of patellar dislocation than the femoral anteversion angle. However, no study has investigated the importance of the femoral trochlea axial orientation in the surgical treatment of patellar dislocation. It is aimed to explore the pathological threshold of the femoral trochlea axial orientation and its guiding implications for surgical interventions in the study.MethodsSixty‐four patients with patellar dislocation and 64 controls were included for measurement of the femoral trochlea axial orientation. The ability to predict the patellar dislocation and the pathologic threshold of the femoral trochlea axial orientation were evaluated using the receiver operating characteristic curve. One hundred patients with medial patellofemoral ligament reconstruction and 25 patients with derotational distal femur osteotomy were divided into two groups based on the femoral trochlea axial orientation cut‐off value and their postoperative knee functions, and patellar tilt angles were compared.ResultsThere were significant differences in the femoral trochlea axial orientation (60.8 ± 7.9 vs. 67.8 ± 4.6, p < 0.05) between patients with patellar dislocation and the normal population. The sensitivity and specificity of the femoral trochlea axial orientation were 0.641 and 0.813, respectively, at the femoral trochlea axial orientation smaller than 63.8°. Amongst patients having had isolated medial patellofemoral ligament reconstruction with decreased femoral trochlea axial orientation, knee function was poorer after surgery. The prognosis of patients with the femoral trochlea axial orientation correction in derotational distal femur osteotomy was better than that for patients without correction.ConclusionsThe femoral trochlea axial orientation had good predictive efficiency for patellar dislocation. Isolated medial patellofemoral ligament reconstruction is not sufficiently effective for patients with patellar dislocation and decreased femoral trochlea axial orientation. Patients with a decreased femoral trochlea axial orientation can have better surgical outcomes after correction by derotational distal femur osteotomy.Level of EvidenceLevel III.
Common radiographic indices used to measure patellar height do not consistently identify patella alta and lack interchangeability between measurements
Authors: Hunter Collin D. R. , Khalil Ameen Z. , Rosenthal Reece M. , Metz Allan K. , Featherall Joseph , Ernat Justin J. , Aoki Stephen K. 
Published: May 2024

AbstractPurposeAbnormal patellar height has been identified as a source of aberrant mechanical functioning within the patellofemoral joint. The purpose of this study is to examine the statistical agreement among three commonly used classification methods: Blackburne‐Peel (BPI), Caton‐Deschamps (CDI) and Insall‐Salvati (ISR), by evaluating (1) the rates of patella alta identification and (2) the ability for one index to predict another.MethodsOne hundred lateral knee radiographs were evaluated using BPI, CDI and ISR to classify each knee as patella normal, patella alta or patella baja. Linear regression analysis was performed to evaluate the relationship between each index. Conversion equations were then derived using the reported linear regression best‐fit line, comparing each pair of indices.ResultsPatella alta was identified in 15 knees using BPI, 15 using CDI and 25 using ISR. A total of seven knees were classified as patella alta by all BPI, CDI and ISR. Statistical analysis revealed significant correlation (p ≤ 0.001) among BPI and CDI (R2 = 0.706), BPI and ISR (R2 = 0.328) and CDI and ISR (R2 = 0.288). Wilcoxon Signed‐Rank test between the three indices revealed no significant difference between the means of converted and original indices.ConclusionDespite their significant correlations and adequate reproducibility, variability between common patellar height indices render predictions and conversions between BPI, CDI and ISR inequivalent. Users of these indices must be aware of their incongruent properties when considering application to patients in the clinical setting. Furthermore, it remains unclear which patellar height measurement technique is the correct index to use in a given knee. This study highlights the need for further investigation to create a reliable and standardised method for identifying patella height.Level of EvidenceLevel IV.
Enhancing robotic precision in medial UKA: Image‐based robot‐assisted system had higher accuracy in implant positioning than imageless robot‐assisted system across 292 knees
Authors: Gaggiotti Stefano , Foissey Constant , Pineda Tomas , Batailler Cécile , Gaggiotti Gabriel , Gaggiotti Santino , Servien Elvire , Lustig Sébastien 
Published: May 2024

AbstractPurposeThe objective of this study was to compare the degree of accuracy in implant positioning and limb alignment offered by two robot‐assisted (RA) systems: an image‐based robot‐assisted (IBRA) versus an imageless robot‐assisted (ILRA) system for the treatment of medial knee osteoarthritis with unicompartmental knee arthroplasty (UKA).MethodsThis retrospective radiographic study included medial UKAs performed between 2011 and 2023. Radiographic measurements taken preoperatively and at 1‐year postoperative control visit focusing on hip‐knee‐ankle angle (HKA), posterior tibial slope (PTS), tibial component coronal alignment relative to Cartier's angle and restoration of proper joint line (JL) height were analyzed. Outliers for postoperative measurements were defined as follows: HKA <175° or >180°, PTS <2° or >8°, >3° or <−3° alterations in Cartier's angle and ±2 mm changes in the height of the joint line.ResultsThe final sample consisted of 292 medial UKAs: 95 (32.5%) with an IBRA system and 197 (67.5%) with an ILRA system. Implant positioning and limb alignment were more accurate in the group of patients treated with IBRA, HKA (77.9% vs. 67.5%, p = 0.07), PTS (93.7% vs. 82.7%, p = 0.01), restoration of tibial varus relative to Cartier's angle (87.4% vs. 65%, p < 0.001) and restoration of JL height (81.1% vs. 69.5%, p = 0.04).ConclusionMedial UKA surgery using an IBRA system was associated with a higher degree of accuracy in implant positioning and postoperative limb alignment as compared to an ILRA system. This is a valuable contribution to help communicate the advantages of using this surgical technique and improve its reproducibility.Level of EvidenceLevel III, Retrospective cohort study.
Ubiquitous but unique: Water depth and oceanographic attributes shape methane seep communities
Authors: Seabrook Sarah , Torres Marta E. , Baumberger Tamara , Butterfield David , Roe Kevin , Cummings Milo , Crawford Rebecca , Thurber Andrew R. 
Published: May 2024

AbstractIn the past decade, thousands of previously unknown methane seeps have been identified on continental margins around the world. As we have come to appreciate methane seep habitats to be abundant components of marine ecosystems, we have also realized they are highly dynamic in nature. With a focus on discrete depth ranges across the Cascadia Margin, we work to further unravel the drivers of seep‐associated microbial community structure. We found highly heterogenous environments, with depth as a deterministic factor in community structure. This was associated with multiple variables that covaried with depth, including surface production, prevailing oxygen minimum zones (OMZs), and geologic and hydrographic context. Development of megafaunal seep communities appeared limited in shallow depth zones (~ 200 m). However, this effect did not extend to the structure or function of microbial communities. Siboglinid tubeworms were restricted to water depths > 1000 m, and we posit this deep distribution is driven by the prevailing OMZ limiting dispersal. Microbial community composition and distribution covaried most significantly with depth, but variables including oxygen concentration, habitat type, and organic matter, as well as iron and methane concentration, also explained the distribution of the microbial seep taxa. While members of the core seep microbiome were seen across sites, there was a high abundance of microbial taxa not previously considered within the seep microbiome as well. Our work highlights the multifaceted aspects that drive community composition beyond localized methane flux and depth, where environmental diversity adds to margin biodiversity in seep systems.