bluuebunny/crossref_metadata_2025_split huggingface dataset:
MYB4 is the best candidate transcription factor involved in pinosylvin stilbene biosynthesis in Pinus strobus L. cells by fungal elicitor treatment
Authors: Kim Yi Rae , Moon Young Bum , Choi Han Bin , Han Jung Yeon , Choi Han Suk , Shim Sangrea , Choi Yong Eui 
Published: May 2024

Abstract
Key message
Dihydropinosylvin monomethyl ether (DPME) and pinosylvin monomethyl ether (PME) are pinosylvin derivatives that show high nematicidal activity against pine wood nematodes (PWNs). Here, we found that fungal elicitor treatment boosted the production of DPME and PME in cultured Pinus strobus L. cells and investigated the transcription factors (TFs) regulating the genes in the pinosylvin stilbenoid biosynthesis pathway.

Context
The discovery of TFs involved in the synthesis of DPME and PME provides an important clue to understanding the pinosylvin stilbenoid synthesis in pine plants.

Aims
We investigated the best fungal elicitor for the production of DPME and PME and the transcriptional activities of genes involved in PME and DPME biosynthesis in P. strobus L. cells after fungal elicitor treatment.

Methods
The content of DPME and PME in P. strobus cells was examined after treatment with fungal elicitors prepared from seven different species of fungi. Moreover, the role of fungal elicitors in the transcriptional activity of genes involved in DPME and PME biosynthesis was investigated by transcriptome analysis using RNA sequencing.

Results
Penicillium chrysogenum Thorn was the most efficient fungal elicitor for the production of DPME and PME in P. strobus cells among the other fungal species. The accumulation of DPME and PME in P. strobus cells after P. chrysogenum elicitor treatment increased 12.7-fold and 23.7-fold, respectively, compared to the control. Transcriptome analysis revealed that fungal elicitor treatment resulted in enhanced transcription of the PAL, 4CL, STS, PMT, and ACC genes, which are involved in PME and DPME biosynthesis. Some transcription factors belonging to the bHLH, MYB, WRKY, and ERF families showed a high transcription rate after fungal elicitor treatment.

Conclusions
We found that the fungal elicitor is a strong inducer of the accumulation of pinosylvin derivatives in the cells of P. strobus. We selected one unigene (c133966_g3_i1 in the MYB family) as the best candidate TF that regulates the DPME and PME biosynthesis in P. strobus by transcriptome analysis.
Boosting self-efficacy and improving practices for smoking prevention and cessation among South American cancer care providers with a web-based algorithm
Authors: Tamí-Maury Irene , Tundealao Samuel , Noé-Díaz Valeri , Garcia Esperanza , Diaz Vilma , Meier Jennie , Dani Mira , Vidaurre Tatiana 
Published: May 2024

Abstract
Background
Digital technologies have positively impacted the availability and usability of clinical algorithms through the advancement in mobile health. Therefore, this study aimed to determine if a web-based algorithm designed to support the decision-making process of cancer care providers (CCPs) differentially impacted their self-reported self-efficacy and practices for providing smoking prevention and cessation services in Peru and Colombia.

Methods
A simple decision-making tree algorithm was built in REDCap using information from an extensive review of the currently available smoking prevention and cessation resources. We employed a pre-post study design with a mixed-methods approach among 53 CCPs in Peru and Colombia for pilot-testing the web-based algorithm during a 3-month period. Wilcoxon signed-rank test was used to compare the CCPs’ self-efficacy and practices before and after using the web-based algorithm. The usability of the web-based algorithm was quantitatively measured with the system usability scale (SUS), as well as qualitatively through the analysis of four focus groups conducted among the participating CCPs.

Results
The pre-post assessments indicated that the CCPs significantly improved their self-efficacy and practices toward smoking prevention and cessation services after using the web-based algorithm. The overall average SUS score obtained among study participants was 82.9 (± 9.33) [Peru 81.5; Colombia 84.1]. After completing the qualitative analysis of the focus groups transcripts, four themes emerged: limited resources currently available for smoking prevention and cessation in oncology settings, merits of the web-based algorithm, challenges with the web-based algorithm, and suggestions for improving this web-based decision-making tool.

Conclusion
The web-based algorithm showed high usability and was well-received by the CCPs in Colombia and Peru, promoting a preliminary improvement in their smoking prevention and cessation self-efficacy and practices.
An intelligent model to decode students’ behavioral states in physical education using back propagation neural network and Hidden Markov Model
Authors: Li Liyan 
Published: May 2024

AbstractThis paper highlights the need for intelligent analysis of students’ behavioral states in physical education tasks. The hand-ring inertial data is used to identify students’ motion sequence states. First, statistical feature extraction is performed based on the acceleration and angular velocity data collected from the bracelet. After completing the filtering and noise reduction of the data, we perform feature extraction by Back Propagation Neural Network (BPNN) and use the sliding window method for analysis. Finally, the classification capability of the model sequence is enhanced by the Hidden Markov Model (HMM). The experimental results indicate that the classification accuracy of student action sequences in physical education exceeds 96% after optimization by the HMM method. This provides intelligent means and new ideas for future student state recognition in physical education and teaching reform.
Reconstructing and tracing the evolution of the road networks in the Haidai region of China during the Bronze and Early Iron Ages
Authors: Yong Heng , Jia Xin , Li Sijin , Yang Lin , Lee Harry F. , Tang Guoan 
Published: May 2024

Abstract
Reconstructing ancient transportation networks is critical to studying past human mobility patterns. China’s Haidai region was a thriving political and economic hub during the Bronze and Early Iron Ages. We used GIS spatial analysis techniques to build a “Settlement Interaction Model” based on archaeological data from the Haidai region during the Bronze and Early Iron Age (Shang Dynasty, Western Zhou Dynasty, Spring & Autumn Period, and Warring States period). The eight-level road network maps with traffic attributes were distinguished based on topography and settlement size. The total lengths of the road networks were estimated to be 19,112 km in the Shang Dynasty, 35,269 km in the Western Zhou Dynasty, 51,555 km in the Spring & Autumn Period, and 77,456 km in the Warring States Period, with the average road flows of 6.6, 31.7, 42.8, and 75.5, respectively. The Z score and one-sample t-test (p < 0.01) confirmed the reliability of the reconstructed road networks. The Shang Dynasty saw the sporadic appearance of simple road routes. More complex routes emerged during the Western Zhou Dynasty and Spring & Autumn Period. The road networks were finally built during the Warring States Period. The development of road networks was closely related to population growth and urbanization. Exploring methods for reconstructing road networks may help us uncover ancient road networks and better understand ancient cultural exchanges.
Superiority of 18F-FAPI-42 PET/CT in the detection of primary tumor and management of appendiceal neoplasm to 18F-FDG PET/CT and CE-CT
Authors: Dong Ye , Huang Shun , Wu Hubing , Cao Min , Huang Yanchao , Tang Ganghua , Zhou Wenlan 
Published: May 2024

Abstract
Background
In the present study, we investigated the value of 18F-fibroblast-activation protein inhibitor (FAPI) positron emission tomography/computed tomography (18F-FAPI-42 PET/CT) to preoperative evaluations of appendiceal neoplasms and management for patients.

Methods
This single-center retrospective clinical study, including 16 untreated and 6 treated patients, was performed from January 2022 to May 2023 at Southern Medical University Nanfang Hospital. Histopathologic examination and imaging follow-up served as the reference standard. 18F-FAPI-42 PET/CT was compared to 18F-fluorodeoxyglucose (18F-FDG) PET/CT and contrast-enhanced CT (CE-CT) in terms of maximal standardized uptake value (SUVmax), diagnostic efficacy and impact on treatment decisions.

Results
The accurate detection of primary tumors and peritoneal metastases were improved from 28.6% (4/14) and 50% (8/16) for CE-CT, and 43.8% (7/16) and 85.0% (17/20) for 18F-FDG PET/CT, to 87.5% (14/16) and 100% (20/20) for 18F-FAPI-42 PET/CT. Compared to 18F-FDG PET/CT, 18F-FAPI-42 PET/CT detected more regions infiltrated by peritoneal metastases (108 vs. 43), thus produced a higher peritoneal cancer index (PCI) score (median PCI: 12 vs. 5, P < 0.01). 18F-FAPI-42 PET/CT changed the intended treatment plans in 35.7% (5/14) of patients compared to CE-CT and 25% (4/16) of patients compared to 18F-FDG PET/CT but did not improve the management of patients with recurrent tumors.

Conclusions
The present study revealed that 18F-FAPI-42 PET/CT can supplement CE-CT and 18F-FDG PET/CT to provide a more accurate detection of appendiceal neoplasms and improved treatment decision making for patients.
Decentralization and immunization program in a single-party state: the case of the Lao People’s Democratic Republic
Authors: Viphonephom Phonevilay , Kounnavong Sengchanh , Reinharz Daniel 
Published: May 2024

Abstract
Background
The Lao People’s Democratic Republic (Lao PDR), a lower-middle-income country, lags behind other Southeast Asian countries in immunization coverage for children under two years of age. The organization of health services is a key determinant of the functionality of immunization programs. However, this aspect, and in particular its decentralization component of the healthcare system, has never been studied.

Methods
A case study in the Lao National Immunization Program was performed using a neo-institutional theory-based conceptual framework, highlighting the structure (rules, laws, resources, etc.) and interpretative schemes (dominant beliefs and ideas) that underlie the state of decentralization of the healthcare system that support the conduct of the immunization program. Twenty-two semi-structured interviews were conducted with representative actors from various government levels, external donors, and civil society, in four provinces. Data were complemented with information retrieved from relevant documents.

Results
The Lao healthcare system has a deconcentrated form of decentralization. It has a largely centralized structure, albeit with certain measures promoting the decentralization of its immunization programs. The structure underlying the state of centralization of immunization services provided is coherent with a shared dominant interpretive scheme. However, the rapid economic, technical, and educational changes affecting the country suggest that the coherence between structure and interpretative schemes is bound to change.

Conclusion
Unprecedented opportunities to access quality higher education and the use of social networks are factors in Lao PDR that could affect the distribution of responsibilities of the different levels of government for public health programs such as the National Immunization Program.
Hope in hardship: charting a new course for mental health in Afghanistan
Authors: Tay Alvin Kuowei 
Published: May 2024

SummaryThe long legacy of upheavals and deprivations in Afghanistan and the associated mental health impacts on its people are well documented. A systematic review undertaken by Alemi et al (2023) presents the most comprehensive synthesis to date on this topic. Drawing on their findings, this editorial examines the complex mental health and psychosocial challenges confronted by neglected vulnerable groups such as pregnant and postnatal women, LGBTQ individuals, older adults, ethnic minority groups and Afghan refugees living overseas. It explores the potential challenges in rebuilding a resilient mental health system following the mass exodus of Afghanis. It calls for a whole-of-society approach that extends beyond clinical interventions to address the broader sociocultural and economic factors influencing mental health.
Treatment condition as a moderator and change in trait mindfulness as a mediator of a brief mindfulness ecological momentary intervention for generalized anxiety disorder
Authors: Zainal Nur Hani , Newman Michelle G. 
Published: May 2024

AbstractBackgroundTheories propose that judgment of and reactivity to inner experiences are mediators of the effect of mindfulness-based interventions on generalized anxiety disorder (GAD). However, no study has tested such theories using brief, mindfulness ecological momentary intervention (MEMI). We thus tested these theories using a 14-day MEMI versus self-monitoring app (SM) control for GAD.MethodsParticipants (N = 110) completed self-reports of trait mindfulness (Five Facet Mindfulness Questionnaire), GAD severity (GAD-Questionnaire-IV), and trait perseverative cognitions (Perseverative Cognitions Questionnaire) at prerandomization, posttreatment, and 1-month follow-up (1MFU). Counterfactual mediation analyses with temporal precedence were conducted.ResultsImprovement in pre–post mindfulness domains (acceptance of emotions, describing feelings accurately, acting with awareness, judgment of inner experience, and reactivity to inner experience) predicted pre-1MFU reduction in GAD severity and pre-1MFU reduction in trait perseverative cognitions from MEMI but not SM. MEMI reduced pre–post reactivity to inner experiences (but not other mindfulness domains) significantly more than SM. Only reduced pre–post reactivity significantly mediated stronger efficacy of MEMI over SM on pre-1MFU reductions in GAD severity (indirect effect: β = −2.970 [−5.034, −0.904],p = .008;bpath: β = −3.313 [−6.350, −0.276],p = .033; percentage mediated: 30.5%) and trait perseverative cognitions (indirect effect: β = −0.153 [−0.254, −0.044],p = .008;bpath: β = −0.145 [−0.260, −0.030],p = .014; percentage mediated: 42.7%). Other trait mindfulness domains were non-significant mediators.ConclusionsReactivity to inner experience might be a mindfulness-based intervention change mechanism and should be targeted to optimize brief MEMIs for GAD.
Mapping the Evidence on the Impact of mHealth Interventions on Patient-Reported Outcomes in Patients With Breast Cancer: A Systematic Review
Authors: Frid Santiago , Amat-Fernández Clara , Fuentes-Expósito María Ángeles , Muñoz-Mateu Montserrat , Valachis Antonis , Sisó-Almirall Antoni , Grau-Corral Immaculada 
Published: May 2024

PURPOSE To comprehensively synthesize the existing evidence concerning mHealth interventions for patients with breast cancer (BC). DESIGN On July 30, 2023, we searched PubMed, PsycINFO, and Google Scholar for articles using the following inclusion criteria: evaluation of mHealth interventions in patients with cancer, at least 30 participants with BC, randomized control trials or prospective pre-post studies, determinants of health (patient-reported outcomes [PROs] and quality of life [QoL]) as primary outcomes, interventions lasting at least 8 weeks, publication after January 2015. Publications were excluded if they evaluated telehealth or used web-based software for desktop devices only. The quality of the included studies was analyzed with the Cochrane Collaboration Risk of Bias Tool and the Methodological Index for Non-Randomized Studies. RESULTS We included 30 studies (20 focused on BC), encompassing 5,691 patients with cancer (median 113, IQR, 135.5). Among these, 3,606 had BC (median 99, IQR, 75). All studies contained multiple interventions, including physical activity, tailored information for self-management of the disease, and symptom tracker. Interventions showed better results on self-efficacy (3/3), QoL (10/14), and physical activity (5/7). Lifestyle programs (3/3), expert consulting (4/4), and tailored information (10/11) yielded the best results. Apps with interactive support had a higher rate of positive findings, while interventions targeted to survivors showed worse results. mHealth tools were not available to the public in most of the studies (17/30). CONCLUSION mHealth interventions yielded heterogeneous results on different outcomes. Identifying lack of evidence on clinical scenarios (eg, patients undergoing systemic therapy other than chemotherapy) could aid in refining strategic planning for forthcoming research endeavors within this field.
Should All Patients With Stage IE Gastric Mucosa-Associated Lymphoid Tissue Lymphoma Receive Antibiotic Eradication Therapy for Helicobacter pylori?
Authors: Laoruangroj Chonlada , Habermann Thomas M. , Wang Yucai , King Rebecca L. , Lester Scott C. , Thompson Carrie A. , Witzig Thomas E. 
Published: May 2024

PURPOSE H. pylori eradication therapy (HPE) can lead to tumor regression in H. pylori–positive (HPP) gastric mucosa-associated lymphoid tissue (MALT) lymphoma. However, some patients do not have detectable H. pylori (HP) infection ( H. pylori–negative [HPN]) and the guidelines differ in their initial approach to HPN patients. The National Comprehensive Cancer Network (NCCN) recommends proceeding to radiation therapy, whereas European Society for Medical Oncology suggests HPE for every patient, even those who are HPN. To address this issue, we evaluated the effectiveness of HPE in limited-stage gastric MALT lymphoma. MATERIALS AND METHODS We retrospectively reviewed patients newly diagnosed with stage IE gastric MALT lymphoma between January 2002 and December 2022. The primary outcome was the complete remission (CR) rate defined as no macroscopic findings of lymphoma and negative gastric biopsy at the follow-up gastric endoscopy. RESULTS Fifty-two patients were reviewed, and HP infection was detected in 19 (36.5%) patients—14 by immunostaining, three by serology, and one each by stool antigen and urea breath test. All 19 HPP and eight of the 33 HPN patients received HPE treatment. The CR rate was 63% (12/19) in HPP patients and 13% (1/8) in HPN patients ( P = .033). After a median follow-up of 89.7 months, only two of the 12 HPP patients achieving CR have relapsed; the one HPN patient who received HPE remains in CR at 12+ months. CONCLUSION For limited-stage HPP gastric MALT lymphoma, HPE is an effective and durable first-line treatment and should be used. For HPN patients, the CR rate with HPE is very low in our experience and is thus in support of the NCCN guideline.
Optimization of Hypericum Perforatum Microencapsulation Process by Spray Drying Method
Authors: Seyrekoğlu Fadime , Temiz Hasan , Eser Ferda , Yıldırım Cengiz 
Published: May 2024

AbstractHypericum perforatum (HP) contains valuable and beneficial bioactive compounds that have been used to treat or prevent several illnesses. Encapsulation technology offers protection of the active compounds and facilitates to expose of the biologically active compounds in a controlled mechanism. Microcapsulation of the hydroalcoholic gum arabic and maltodextrin have hot been used as wall materials in the encapsulation of HP extract. Therefore, the optimum microencapsulation parameters of Hypericum perforatum (HP) hydroalcoholic extract were determined using response surface methodology (RSM) for the evaluation of HP extract. Three levels of three independent variables were screened using the one-way ANOVA. Five responses were monitored, including total phenolic content (TPC), 2,2-Diphenyl-1-picrylhydrazyl (DPPH), carr index (CI), hausner ratio (HR), and solubility. Optimum drying conditions for Hypericum perforatum microcapsules (HPMs) were determined: 180 °C for inlet air temperature, 1.04/1 for ratio of maltodextrin to gum arabic (w/w), and 1.98/1 for coating to core material ratio (w/w). TPC, antioxidant activity, CI, HR, and solubility values were specified as 316.531 (mg/g GAE), 81.912%, 6.074, 1.066, and 35.017%, respectively, under the optimized conditions. The major compounds of Hypericum perforatum (hypericin and pseudohypericin) extract were determined as 4.19 μg/g microcapsule and 15.09 μg/g microcapsule, respectively. Scanning electron microscope (SEM) analysis revealed that the mean particle diameter of the HPMs was 20.36 µm. Based on these results, microencapsulation of HPMs by spray drying is a viable technique which protects the bioactive compounds of HP leaves, facilitating its application in the pharmaceutical, cosmetic, and food industries.
Graphical Abstract
The (Surprising) Sample Optimality of Greedy Procedures for Large-Scale Ranking and Selection
Authors: Li Zaile , Fan Weiwei , Hong L. Jeff 
Published: May 2024

Ranking and selection (R&S) aims to select the best alternative with the largest mean performance from a finite set of alternatives. Recently, considerable attention has turned toward the large-scale R&S problem which involves a large number of alternatives. Ideal large-scale R&S procedures should be sample optimal; that is, the total sample size required to deliver an asymptotically nonzero probability of correct selection (PCS) grows at the minimal order (linear order) in the number of alternatives, k. Surprisingly, we discover that the naïve greedy procedure, which keeps sampling the alternative with the largest running average, performs strikingly well and appears sample optimal. To understand this discovery, we develop a new boundary-crossing perspective and prove that the greedy procedure is sample optimal for the scenarios where the best mean maintains at least a positive constant away from all other means as k increases. We further show that the derived PCS lower bound is asymptotically tight for the slippage configuration of means with a common variance. For other scenarios, we consider the probability of good selection and find that the result depends on the growth behavior of the number of good alternatives: if it remains bounded as k increases, the sample optimality still holds; otherwise, the result may change. Moreover, we propose the explore-first greedy procedures by adding an exploration phase to the greedy procedure. The procedures are proven to be sample optimal and consistent under the same assumptions. Last, we numerically investigate the performance of our greedy procedures in solving large-scale R&S problems. This paper was accepted by Baris Ata, stochastic models and simulation. Funding: This work was supported by the National Natural Science Foundation of China [Grants 72091211, 72071146, 72161160340]. Supplemental Material: The e-companion and data files are available at https://doi.org/10.1287/mnsc.2023.00694 .