bluuebunny/crossref_metadata_2025_split huggingface dataset:
Constraints on brittle field exhumation of the Everest‐Makalu section of the Greater Himalayan Sequence: Implications for models of crustal flow
Authors: Streule Michael J. , Carter Andrew , Searle Michael P. , Cottle John M. 
Published: April 2012

New apatite and zircon fission track (FT) data from the summit slopes of Everest and along the Barun, Arun, Dudh Kosi, and Kangshung valleys that drain the Everest and Makalu massifs cover a vertical sample transect of almost 8000 m of the Eastern Nepal Greater Himalayan Sequence (GHS). Apatite FT ages range from 0.9 ± 0.3 Ma to 3.1 ± 0.3 Ma in the GHS with ages increasing systematically with elevation. Apatite FT ages in the Everest Series and summit Ordovician limestones are much older, up to 30.5 ± 5.1 Ma. Zircon FT ages from the GHS range from 3.8 ± 0.4 Ma to 16.3 ± 0.8 Ma. The brittle exhumation rates calculated from these data show the GHS was exhumed, since ∼9 Ma, at an average rate of 1.0 ± 0.2 mm/a. Pliocene exhumation rates are higher at 1.7 ± 0.3 mm/a. These values are not significantly different from the estimate of ductile exhumation rates of 1.8 mm/a recorded by metamorphic minerals undergoing decompression between 18.7 and 15.6 Ma but are well below the values (up to 10 mm/a) used by thermomechanical models for ductile channel flow in the GHS. If representative of the GHS these models will therefore require further ‘tuning’. Higher exhumation rates in the Pliocene have also been observed in other parts of the Himalaya and points to a regional cause, likely increased erosion due to the onset of late Pliocene–Pleistocene glaciation of the high Himalaya.
Increasing subsurface water storage in discontinuous permafrost areas of the Lena River basin, Eurasia, detected from GRACE
Authors: Velicogna I. , Tong J. , Zhang T. , Kimball J. S. 
Published: April 2012

We use monthly measurements of time‐variable gravity from the GRACE (Gravity Recovery and Climate Experiment) satellite mission to quantify changes in terrestrial water storage (TWS) in the Lena river basin, Eurasia, during the period April 2002 to September 2010. We estimate a TWS increase of 32 ± 10 km3/yr for the entire basin, equivalent to an increase in water thickness of 1.3 ± 0.4 cm/yr over a basin of 2.4 million km2. We compare TWS estimates from GRACE with time series of precipitation (P) minus evapotranspiration (ET) from ERA‐Interim reanalysis minus observational river discharge (R). We find an excellent agreement in annual and inter‐annual variability between the two time series. Furthermore, we find that a bias of −20 ± 10% in P‐ET is sufficient to effectively close the water budget with GRACE. When we account for this bias, the time series of cumulative TWS from GRACE and climatological data agree to within ±3.8 cm of water thickness, or ±9% of the mean annual P. The TWS increase is not uniform across the river basin and exhibits a peak, over an area of 502,400 km2, centered at 118.5°E, 62.5°N, and underlain by discontinuous permafrost. In this region, we attribute the observed TWS increase of 68 ± 19 km3 to an increase in subsurface water storage. This large subsurface water signal will have a significant impact on the terrestrial hydrology of the region, including increased baseflow and alteration of seasonal runoff.
Transition states of native and drug-resistant HIV-1 protease are the same
Authors: Kipp D. Randal , Hirschi Jennifer S. , Wakata Aya , Goldstein Harris , Schramm Vern L. 
Published: April 2012

HIV-1 protease is an important target for the treatment of HIV/AIDS. However, drug resistance is a persistent problem and new inhibitors are needed. An approach toward understanding enzyme chemistry, the basis of drug resistance, and the design of powerful inhibitors is to establish the structure of enzymatic transition states. Enzymatic transition structures can be established by matching experimental kinetic isotope effects (KIEs) with theoretical predictions. However, the HIV-1 protease transition state has not been previously resolved using these methods. We have measured primary
14
C and
15
N KIEs and secondary
3
H and
18
O KIEs for native and multidrug-resistant HIV-1 protease (I84V). We observed
14
C KIEs (
14
V
/
K
) of 1.029 ± 0.003 and 1.025 ± 0.005,
15
N KIEs (
15
V
/
K
) of 0.987 ± 0.004 and 0.989 ± 0.003,
18
O KIEs (
18
V
/
K
) of 0.999 ± 0.003 and 0.993 ± 0.003, and
3
H KIEs (
3
V
/
K
) KIEs of 0.968 ± 0.001 and 0.976 ± 0.001 for the native and I84V enzyme, respectively. The chemical reaction involves nucleophilic water attack at the carbonyl carbon, proton transfer to the amide nitrogen leaving group, and C-N bond cleavage. A transition structure consistent with the KIE values involves proton transfer from the active site Asp-125 (1.32 Å) with partial hydrogen bond formation to the accepting nitrogen (1.20 Å) and partial bond loss from the carbonyl carbon to the amide leaving group (1.52 Å). The KIEs measured for the native and I84V enzyme indicate nearly identical transition states, implying that a true transition-state analogue should be effective against both enzymes.
Concordance between Urinary Cotinine Levels and Self-Reported Tobacco Use among Drug-Dependent Persons: A Pilot Study
Authors: Jain Raka , Balhara Yatan Pal Singh , Jhanjee Sonali , Sethi Hem 
Published: April 2012

Self-reported drug use is a principle measure in the evaluation of treatment outcome. As there have been concerns about the accuracy of self-reporting, it is necessary to establish its validity by an objective method. The aim of the study was to examine the concordance between urinary cotinine concentrations and self-reported tobacco use among drug dependents seeking treatment at National Drug Dependence Treatment Centre, AIIMS, New Delhi, India. Eighty consecutive male drug addicts attending the OPD at National Drug Dependence Treatment Centre were interviewed by the clinician. Their tobacco as well as drug use history was recorded, and thereafter their urine sample was analyzed for drug testing. Mean age of the subjects was found to be 33 years (SD: 10). Urinalysis showed high concentration of cotinine (mean ± SD: 586.40 ± 222.15 ng/ml) in 95% of the subjects. High concordance was observed between self-report of tobacco use and urinary cotinine. The quantity of tobacco consumption and FTND scores were also correlated with the urinary cotinine levels. Urinalysis also showed misuse of opioids, benzodiazepines and antihistaminics. The treatment for tobacco use has been given low priority in the de-addiction centers. Tobacco is highly prevalent among the drug abusers. The effectiveness of the treatment program may be increased by using the combination of urine analysis along with self-report.
Occurrence of Impatiens necrotic spot virus and Tomato spotted wilt virus on Potatoes in Iran
Authors: Pourrahim R. , Golnaraghi A. R. , Farzadfar Sh. 
Published: April 2012

There have been an increasing number of records of the natural infection of various crops and ornamentals in Iran with Impatiens necrotic spot virus (INSV), Tomato spotted wilt virus (TSWV), and Tomato yellow fruit ring virus (TYFRV), a recently proposed species in the genus Topovirus (3). TYFRV, originally believed to be TSWV and named as such, has been previously reported to occur in Iranian potato fields (2). During the growing seasons of 2004 to 2006, surveys were conducted in potato fields in different potato-producing (Solanum tuberosum) provinces of Iran (Ardabil, Azarbayejan-e-sharqi, Chaharmahal-va-bakhtiyari, Esfahan, Hamedan, Kerman, Khorasan, Khuzestan, Lorestan, Tehran, Qazvin, and Zanjan) to detect the presence of Tospovirus spp. infecting this crop, including Groundnut ringspot virus (GRSV), INSV, Iris yellow spot virus (IYSV), Tomato chlorotic spot virus (TCSV), TSWV, TYFRV, and Watermelon silver mottle virus (WSMoV). Overall, 186 fields were surveyed, and 2,823 potato leaf samples from plants showing tospovirus-like symptoms of chlorotic or necrotic spots, chlorosis, and necrosis were collected before or through the flowering stage, approximately 50 to 90 days after planting. Each leaf sample was tested by double-antibody sandwich (DAS)-ELISA using specific antisera (Bioreba, Reinach, Switzerland; Loewe, Sauerlach, Germany; DSMZ, Braunschweig, Germany) for the presence of the aforementioned tospoviruses. TYFRV, TSWV, and INSV were found in 24.0, 4.1, and 0.4% of the samples collected from 133, 51, and 7 fields surveyed, respectively. None of the samples had a positive reaction in ELISA to GRSV, IYSV, TCSV, and WSMoV. To confirm this testing, a number of the leaf samples that were found to be positive for INSV, TSWV, and TYFRV in ELISA tests were mechanically inoculated on Petunia × hybrid and Nicotiana benthamiana; the inoculated plants showed typical necrotic local lesions of tospoviruses and chlorotic or necrotic spots followed by systemic infection, respectively; their infection was subsequently confirmed by ELISA. The samples also were tested by reverse transcription-PCR technique using previously described specific primers (1,4). The PCR reaction resulted in the specific amplification of a 0.59-, 0.71-, and 0.67-kb (or 1.2-kb) fragment of INSV, TSWV, and TYFRV RNAs, respectively. This study showed that tospoviruses, especially TYFRV, are widespread in Iranian potato fields. It is hoped that the results may help us to improve a seed potato certification program in the future. To our knowledge, this is the first report of INSV and TSWV from potatoes in Iran. References: (1) A. R. Golnaraghi et al. Plant Dis. 92:1280, 2008. (2) R. Pourrahim et al. Plant Dis. 84:442, 2001. (3) S. Winter et al. Plant Pathol. 55:287, 2006. (4) H. Uga and S. Tsuda. Phytopathology 95:166, 2005.
First Report of Cylindrocarpon macrodidymum Associated with Black Foot Diseases of Grapevine in Turkey
Authors: Özben S. , Demirci F. , Değirmenci K. , Uzunok S. 
Published: April 2012

Grape (Vitis vinifera) is widely planted and is an economically important crop in Turkey for domestic consumption and export. Black foot disease, caused by Cylindrocarpon macrodidymum Halleen, Schroers & Crous, is a recently identified but worsening problem in vineyards worldwide (3,4). Symptomatic grapevines show reduced vigor, shortened internodes, small leaves with interveinal chlorosis, and necrosis frequently leading to the death of the plants (1). Roots of symptomatic grapevines exhibit black, sunken, necrotic lesions with a reduction in root biomass. Pith of affected vines is discolored (4). During the summers of 2009 and 2010, a survey was carried out in 63 vineyards (4 to 15 years old) in six locations of Ankara Province. We collected 44 samples from roots and crowns of grapevines exhibiting black foot symptoms. In cross section, extensive necrosis at the base of the trunk and brown-black spots in xylem vessels were observed, resembling those previously reported for black foot disease (2,4). Isolations were made from roots, vascular elements, and pith tissue. In this study, 26 isolates were identified as C. macrodidymum on the basis of morphological characteristics. Isolates identified as C. macrodidymum had a dark orange-brown colony color and abundant aerial mycelia when grown on potato dextrose agar. Isolates produced ellipsoid or ovoid microconidia. The macroconidia were one to three septate, straight, and cylindrical. One-septate macroconidia were 24 to 32 × 5 to 7 μm; three-septate macroconidia were 26 to 40 × 5 to 6 μm. Chlamydospores developed in short, intercalary chains. Conidiophores were simple or complex and sporodochial. Isolate identities were confirmed by sequence analysis of the ribosomal DNA internal transcribed spacer (GenBank Accession No. HM245331) with primers ITS1 and ITS4 (4). Isolates had 99% genetic identity with other isolates of C. macrodidymum present in GenBank. In pathogenicity tests, one representative isolate was used to inoculate five grapevine plants. Tests were completed by drench inoculation onto 3-month-old rooted cuttings of cv. Sultana with 25 ml of a conidia suspension (106 conidia ml–1). Controls were inoculated with an equal volume of sterile distilled water. Plants were incubated for 4 months in a controlled environment facility at 25°C. After 3 to 4 months, inoculations resulted in reduction of root mass, and C. macrodidymum was reisolated from regions of brown streaking in wood and discolored vascular tissue in all inoculated plants, fulfilling Koch's postulates. Control plants were asymptomatic and C. macrodidymum was not recovered from control plants. To our knowledge, this is the first report of the presence of C. macrodidymum causing black foot disease on grapevine in Turkey. References: (1) S. Alaniz et al. Plant Dis. 93:821, 2009. (2) F. Hallen et al. Stud. Mycol. 50:431, 2004. (3) F. Halleen et al. Phytopathol. Mediterr. 45:S55, 2006. (4) E. Petit and W. D. Gubler. Plant Dis. 89:1051, 2005.
NaJAZh Regulates a Subset of Defense Responses against Herbivores and Spontaneous Leaf Necrosis inNicotiana attenuataPlants
Authors: Oh Youngjoo , Baldwin Ian T. , Gális Ivan 
Published: April 2012

AbstractThe JASMONATE ZIM DOMAIN (JAZ) proteins function as negative regulators of jasmonic acid signaling in plants. We cloned 12 JAZ genes from native tobacco (Nicotiana attenuata), including nine novel JAZs in tobacco, and examined their expression in plants that had leaves elicited by wounding or simulated herbivory. Most JAZ genes showed strong expression in the elicited leaves, but NaJAZg was mainly expressed in roots. Another novel herbivory-elicited gene, NaJAZh, was analyzed in detail. RNA interference suppression of this gene in inverted-repeat (ir)JAZh plants deregulated a specific branch of jasmonic acid-dependent direct and indirect defenses: irJAZh plants showed greater trypsin protease inhibitor activity, 17-hydroxygeranyllinalool diterpene glycosides accumulation, and emission of volatile organic compounds from leaves. Silencing of NaJAZh also revealed a novel cross talk in JAZ-regulated secondary metabolism, as irJAZh plants had significantly reduced nicotine levels. In addition, irJAZh spontaneously developed leaf necrosis during the transition to flowering. Because the lesions closely correlated with the elevated expression of programmed cell death genes and the accumulations of salicylic acid and hydrogen peroxide in the leaves, we propose a novel role of the NaJAZh protein as a repressor of necrosis and/or programmed cell death during plant development.
Bimodal transparency as an indicator for alternative states in South American lakes
Authors: KOSTEN SARIAN , VERNOOIJ MARTINE , VAN NES EGBERT H. , SAGRARIO MARÍA DE LOS Á. G. , CLEVERS JAN G. P. W. , SCHEFFER MARTEN 
Published: April 2012

Summary1. The alternative state theory claims that shallow lakes may have either clear water, and be dominated by submerged macrophytes, or turbid water and be dominated by phytoplankton. Most evidence for this theory comes from studies in temperate or boreal regions of Europe. Because of differences in the strength of trophic interactions, such as in the pressure of zooplankton grazing on phytoplankton, this influential theory might not apply elsewhere.2. Here, we test the theory for South American lakes, combining field data and Landsat satellite data. We studied the frequency distribution of primary producers and water transparency, looking for potential bimodality separating clear and turbid lakes. A bimodal distribution might be observed if there are indeed alternative states, although would not itself be sufficient evidence for the theory. Possible shifts between alternative states were analysed by comparing satellite data from 1987 to 2005.3. In our field data, there was a bimodal pattern in phytoplankton abundance and possibly in the abundance of submerged macrophytes, but not in water transparency. Analyses of the larger satellite data set revealed bimodality in lake transparency in 2005, but less so in 1987. In 1987, the lakes were generally clearer, and the transition to higher turbidity was more gradual than in 2005. The stronger bimodality in the more recent data, and the overall lower transparency, could have been caused by an increase in fertiliser use and subsequent eutrophication but also by differences in hydrology. Further, 1987 was much wetter than 2005, which could have caused dilution of suspended particles, leading to clearer water.4. While a bimodal distribution in the abundance of primary producers and water clarity is not decisive evidence for or against the theory of alternative states, our data clearly fail to refute it.
Increasing trends in childlessness in recent birth cohorts – a registry‐based study of the total Danish male population born from 1945 to 1980
Authors: Priskorn L. , Holmboe S. A. , Jacobsen R. , Jensen T. K. , Lassen T. H. , Skakkebaek N. E. 
Published: April 2012

SummaryThe fertility rate has recently declined in many parts of the World, including Europe. To a large extent, this change can be explained by the socio‐economic development. However, increasing fertility problems and widespread occurrence of poor semen quality could in part explain the few births. The objective of this registry based study was to investigate birth cohort related trends in fertility and childlessness among Danish men. The study population comprised all 1 616 677 men in Denmark born from 1945 to 1980 of whom 1 359 975 (84.1%) were native Danes. Data were obtained from Statistics Denmark and contained information from The National Danish Birth Registry and The Danish In Vitro Fertilization (IVF) Registry. For consecutive birth cohorts of native Danish men cumulative fertility rates at age 45 declined from 1.91 children per man in the 1945 birth cohort to 1.71 for men born in 1960. The proportion of childless men at age 45 increased from 14.8% to 21.9% in the same birth cohorts. Assisted reproductive technology (ART) seemed to compensate partly for the lower fertility and to reduce the proportion of childless men. In contrast, recent reports on corresponding birth cohorts of Danish women showed that the proportion remaining childless throughout life has been lower than in men and has not shown a similar increase. In conclusion, using unique Danish registries the study showed a birth cohort related decline in fertility rates and an increase in childlessness among men. In the more recent cohorts more than one in five men remained childless. The causes behind the findings are likely multi‐factorial. Hitherto, most attention has been given to socio‐economic factors which undoubtedly play a major role. Our findings lend support to the hypothesis that the high prevalence of low sperm counts among young Danish men may be a contributing factor.
Reduced artery diameters in Klinefelter syndrome
Authors: Foresta C. , Caretta N. , Palego P. , Ferlin A. , Zuccarello D. , Lenzi A. , Selice R. 
Published: April 2012

SummaryVarious epidemiological studies in relatively large cohorts of patients with Klinefelter syndrome (KS) described the increased morbidity and mortality in these subjects. Our aim was to study the structure and function of arteries in different districts to investigate in these subjects possible alterations. A total of 92 patients having non‐mosaic KS, diagnosed in Centre for Human Reproduction Pathology at the University of Padova, and 50 age‐matched healthy male controls were studied. Klinefelter syndrome subjects and controls evaluation included complete medical history, physical examination, measurement of concentrations of the reproductive hormones, lipidic and glycidic metabolism, AR function and sensitivity, ultrasound examinations (diameters, carotid intima‐media thickness and brachial flow‐mediated dilation) of brachial, common carotid and common femoral artery and abdominal aorta. Klinefelter syndrome patients showed significantly reduced artery diameters in all districts evaluated. On the contrary no statistically significant difference was found in cIMT and brachial FMD values between KS patients and controls. Furthermore, we found no statistically significant correlation of artery diameters with reproductive hormones, metabolic parameters, anthropometric measures and weighted CAG repeats. To our knowledge, this is the first study finding a reduced artery diameter in several districts in KS patients compared with that of normal male subjects and overlapping to that of female subjects. We have not an explanation for this phenomenon, even if a possible involvement of genes controlling the development of vascular system might be hypothesized, and further research is required to verify this hypothesis.
Pretreatment of aqueous pectin solution by cross‐flow microfiltration: analysis of operational parameters, degree of concentration and pectin losses
Authors: da Silva Vítor R. , Hamerski Fabiane , Scheer Agnes P. 
Published: April 2012

SummaryObtaining pectin through traditional precipitation processes requires a large amount of organic solvent. A reduction in solvent consumption may be achieved by incorporating a cross‐flow microfiltration step in which the extraction solution is removed and pectin is concentrated. In this study, we used α‐alumina tubular membrane (0.44 μm) in two operation modes: total recycle mode to evaluate the effect of temperature, initial concentration of pectin and transmembrane pressure on the permeate flux and pectin coefficient rejection; and batch mode to evaluate the degree of concentration and loss of pectin. It was observed that pectin coefficient rejection varied from 93.4 ± 0.7% to 97.8 ± 0.3%, and a maximum permeate flux of 238.69 ± 6.48 kg m−2 h−1 with 0.12 MPa at 50 °C and 1.0 g kg−1. Using the optimum conditions, the flux observed at the end of the process was 32.8% lower than the flux in the total recycle mode, enabling a final concentration of pectin in the retained solution of 61.04 ± 0.87% with an average pectin loss in the permeate stream of 8.18%.
Effect of processing of corn for production of masa, tortillas and tortilla chips on the scavenging capacity of reactive nitrogen species
Authors: López‐Martínez Leticia X. , Parkin Kirk L. , Garcia Hugo S. 
Published: April 2012

SummaryThe effect of processing conventional and pigmented corn into masa, tortilla and tortilla chips on the ability to scavenge nitric oxide (NO) and peroxynitrite (ONOO−) was investigated. The level of retention of nitric oxide and peroxynitrite (mediated for inhibition of nitrite formation scavenging capacity for masa, tortilla and tortilla chips) ranged 56.2–78.2%, 67.4–45%, 40.3–62.0% (for nitric oxide) and 38.6–81.7%, 23.3–47.7%, 19.3–67.2% (for inhibition of nitrite formation), respectively. The antinitrosative activities were affected significantly (P > 0.05) by nixtamalisation, but not by processing masa into tortilla and tortilla chips (P > 0.05). The yellow variety and its corresponding products showed the greatest capacity to scavenge nitric oxide and inhibition of nitrite formation among conventional varieties and the purple variety ranked highest among the pigmented varieties.
Influence of pH on colour and iron content of peptide fractions obtained from bovine Hb concentrate hydrolysates
Authors: Cian Raúl E. , Drago Silvina R. , González Rolando J. 
Published: April 2012

SummaryThe effect of pH on colour and iron content (Fe) of peptide fractions obtained from bovine haemoglobin concentrate (BHC) hydrolysates was studied. Four hydrolysates were obtained using three enzymes: Protex‐6‐L (P), Fungal–Protease–Concetrate (FC) and Flavourzyme (F). BHC and its hydrolysates (P, FC, P + F, FC + F) were fractioned at pH 4.5, 7.0 and 9.5. Solubility and Fe from different fractions were measured. Correlations between CIELAB colour parameters and Fe from different fractions were analysed. The colour from different fractions varied from red to yellow (a* and b* positives). Lightness values (L*) ranged from twenty‐four to seventy. FC4.5 and FC + F4.5 fractions were the clearest and yellow (higher L*, b*, h), while BHC9.5 and P + F9.5 fractions had the lowest values of L*, b* and h. There was an inverse linear relationship between b* and L* parameters and Fe from fractions. This relationship could be associated with the pH of extraction. As pH increases Fe significantly increases and lower b* and L* values were obtained.