bluuebunny/crossref_metadata_2025_split huggingface dataset:
Growing pains: Twin family study evidence for genetic susceptibility and a genetic relationship with restless legs syndrome
Authors: Champion D. , Pathirana S. , Flynn C. , Taylor A. , Hopper J.L. , Berkovic S.F. , Jaaniste T. , Qiu W. 
Published: March 2012

AbstractBackgroundGrowing pains (GP) is a prevalent familial childhood disorder of unknown aetiology. Familial occurrence of GP, and individual and familial association of GP with restless legs syndrome (RLS) has been reported.MethodsWe applied a twin family design to search for evidence of genetic susceptibility to GP, and for a genetic relationship between GP and RLS. The parents of 1843 twin pairs aged 3–16 years were administered a questionnaire, which identified 88 pairs with at least one twin individual fulfilling criteria for GP. Standard questionnaires for history of GP and RLS were completed for these twin pairs, their siblings and parents.ResultsTwenty‐five of 34 monozygotic (MZ) pairs were concordant for GP, compared with 12 of the 54 dizygotic (DZ) pairs. The casewise concordance was 0.85 and 0.36 for MZ and DZ pairs, respectively (p < 0.001). The lifetime GP prevalence for relatives of twins with GP was 51% for non‐twin siblings, 47% for parents. Twenty‐three percent of twin individuals with GP met RLS criteria compared with 8% of twin individuals without GP (p = 0.03). Of the twins with GP concordance, 19% met RLS criteria compared with 2% of twins with GP discordance (p = 0.01). In two MZ pairs, one had GP and the other RLS. The lifetime prevalence of RLS was 40% for mothers, and 24% for fathers and 18% for non‐twin siblings.ConclusionThis first twin family study of GP provides evidence for a genetic aetiology and for a genetic relationship to RLS.
Pain‐related catastrophizing in pain patients and people with pain in the general population
Authors: de Boer M.J. , Struys M.M.R.F. , Versteegen G.J. 
Published: March 2012

AbstractBackgroundCatastrophizing is a defining factor in the pain experience and strongly contributes to the prediction of various aspects of health. Catastrophizing is not just present in pain patients, but may also be present in people with non‐clinical pain. The aim of the present study is to investigate levels of catastrophizing in pain patients and people with pain from the general population. Also, the relationship between catastrophizing and pain intensity, specialist consultation and use of pain medication is studied.MethodsA cross‐sectional survey design was used, including 150 pain patients and 376 respondents from the general population.ResultsThe results show that pain is highly prevalent in the general population. The pain patients reported significantly higher levels of pain intensity and catastrophizing than the community pain sample. In both samples, significant positive correlations between catastrophizing and pain intensity were found. The data show a linear relationship between catastrophizing and pain intensity, which can be interpreted as evidence for a dose–response pattern in pain‐related catastrophizing. Furthermore, catastrophizing predicted specialist consultation and medication use in the community pain sample.ConclusionsIt can be concluded that pain‐related catastrophizing is present in pain patients as well as people with pain from the general population in a dose–response pattern. Catastrophizing seems to be an important factor determining certain aspects of pain‐related medical consumption, even in non‐clinical pain, and should therefore be a target of the screening procedure and early intervention.
Ciliogenic RFX transcription factors regulate FGF1 gene promoter
Authors: Hsu Yi‐Chao , Kao Chien‐Yu , Chung Yu‐Fen , Chen Mei‐Shu , Chiu Ing‐Ming 
Published: March 2012

AbstractFibroblast growth factor 1 (FGF1) has been shown to regulate cell proliferation, cell division, and neurogenesis. Human FGF1 gene 1B promoter (−540 to +31)‐driven green fluorescence (F1BGFP) was shown to recapitulate endogenous FGF1 gene expression. It can also be used to isolate neural stem/progenitor cells (NSPCs) and glioblastoma stem cells (GBM‐SCs) from developing mouse brains and human glioblastoma tissues, respectively. However, the regulatory mechanisms of FGF‐1B promoter and F1BGFP(+) cells are not clear. In this study, we present several lines of evidence to show the roles of ciliogenic RFX transcription factors in the regulation of FGF‐1B gene promoter and F1BGFP(+) cells: (i) RFX1, RFX2, and RFX3 transcription factors could directly bind the 18‐bp cis‐element (−484 to −467), and contribute to the regulation of FGF1 promoter and neurosphere formation. (ii) We demonstrated RFX2/RFX3 complex could only be detected in the nuclear extract of FGF‐1B positive cells, but not in FGF‐1B negative cells. (iii) Protein kinase C inhibitors, staurosporine and rottlerin, could decrease the percentage of F1BGFP(+) cells and their neurosphere formation efficiency through reducing the RFX2/3 complex. (iv) RNA interference knockdown of RFX2 could significantly reduce the percentage of F1BGFP(+) cells and their neurosphere formation efficiency whereas overexpression of RFX2 resulted in the opposite effects. Taken together, this study suggests ciliogenic RFX transcription factors regulate FGF‐1B promoter activity and the maintenance of F1BGFP(+) NSPCs and GBM‐SCs. J. Cell. Biochem. 113: 2511–2522, 2012. © 2012 Wiley Periodicals, Inc.
Strategies for tissue and organ decellularization
Authors: Gilbert Thomas W. 
Published: March 2012

AbstractDecellularized tissues have been successfully used in a variety of tissue engineering/regenerative medicine applications, and more recently decellularized organs have been utilized in the first stages of organ engineering. The protocols used to decellularize simple tissues versus intact organs differ greatly. Herein, the most commonly used decellularization methods for both surgical mesh materials and whole organs are described, with consideration given to how these different processes affect the extracellular matrix and the host response to the scaffold. J. Cell. Biochem. 113: 2217–2222, 2012. © 2012 Wiley Periodicals, Inc.
Oxygen‐bound hell's gate globin I by classical versus LB nanotemplate method
Authors: Pechkova Eugenia , Scudieri Dora , Belmonte Luca , Nicolini Claudio 
Published: March 2012

AbstractX‐ray atomic structure of recombinant Hell's gate globin I (HGbI) from Methylacidophilum infernorum was calculated from the X‐ray diffraction data of two different types of crystals: obtained by classical hanging drop and by LB nanotemplate method under the same crystallization conditions. After the accurate comparison of crystallographic parameters and electron density maps of two structures they appears to be quite similar, while the quality of the crystals grown by LB nanotemplate method was higher then of those grown by classical method. Indeed, the resolution of the LB crystal structure was 1.65 Å, while classical crystals showed only 3.2 Å resolution. Moreover, the reproducibility of this result in the case of LB crystals was much better—nine crystals from 10 gave the same structural results, while only two of 10 classical crystals were appropriate for the X‐ray structure resolution. J. Cell. Biochem. 113: 2543–2548, 2012. © 2012 Wiley Periodicals, Inc.
Monomeric adenine decay dynamics influenced by the DNA environment
Authors: Lu You , Lan Zhenggang , Thiel Walter 
Published: March 2012

AbstractWe report on‐the‐fly surface‐hopping dynamics simulations of single adenine embedded in solvated DNA oligomers, (dA)10 and (dA)10·(dT)10. Both model systems are found to decay from the S1 to the S0 state via distinct monomeric channels, on account of the strong hydrogen‐bonding interactions between the Watson–Crick pair in the double‐stranded oligomer. Surprisingly, the decay times (several picoseconds) for the current models are 10 times longer than those of adenine in the gas or aqueous phase, while matching one of the time constants observed experimentally. We discuss possible reasons for these longer decay times, including steric hindrance in the DNA strands, electronic effects of the environment, and the presence of other local excited‐state minima. We present optimized geometries and relative energies for representative S0 and S1 minima as well as conical intersections related to the hopping events. We have also computed steady‐state and time‐dependent fluorescence spectra that may help understand the experimental observations. © 2012 Wiley Periodicals, Inc.
Aqua‐planet simulations of the formation of the South Atlantic convergence zone
Authors: Nieto Ferreira Rosana , Chao Winston C. 
Published: March 2012

AbstractThe impact of Amazon Basin convection and cold fronts on the formation and maintenance of the South Atlantic convergence zone (SACZ) is studied using aqua‐planet simulations with a general circulation model. In the model, a circular patch of warm sea‐surface temperature (SST) is used to mimic the effect of the Amazon Basin on South American monsoon convection. The aqua‐planet simulations were designed to study the effect of the strength and latitude of Amazon Basin convection on the formation of the SACZ. The simulations indicate that the strength of the SACZ increases as the Amazon convection intensifies and is moved away from the equator. Of the two controls studied here, the latitude of the Amazon convection exerts the strongest effect on the strength of the SACZ. An analysis of the synoptic‐scale variability in the simulations shows the importance of frontal systems in the formation of the aqua‐planet SACZ. Composite time series of frontal systems that occurred in the simulations show that a robust SACZ occurs when fronts penetrate into the subtropics and become stationary there as they cross eastward of the longitude of the Amazon Basin. Moisture convergence associated with these frontal systems produces rainfall not along the model SACZ region and along a large portion of the northern model Amazon Basin. Simulations in which the warm SST patch was too weak or too close to the equator did not produce frontal systems that extended into the tropics and became stationary, and did not form a SACZ. In the model, the SACZ forms as Amazon Basin convection strengthens and migrates far enough southward to allow frontal systems to penetrate into the tropics and stall over South America. This result is in agreement with observations that the SACZ tends to form after the onset of the monsoon season in the Amazon Basin. Copyright © 2012 Royal Meteorological Society
Detailed spectroscopic analysis of complex multi‐component materials using a combination of Raman mapping with BTEM
Authors: Widjaja Effendi , Garland Marc 
Published: March 2012

AbstractThe combined application of Raman microscopy and self‐modeling curve resolution techniques can address a wide range of material characterization problems. In particular, the combination of Raman microscopy and the Band‐Target Entropy Minimization (BTEM) algorithm has been applied to various organic, inorganic, pharmaceutical and bio‐material related problems. In the present contribution, the principles behind this type of analysis are reviewed, followed by a number of case‐by‐case studies. For each of these examples, a Raman microscopic mapping measurement (consisting of 100 s up to 1000 s of spectra) is performed, followed by BTEM analysis which provides the underlying pure component spectra of the constituents present in the system without the use of any a priori information. In most cases, outstanding signal‐to‐noise ratios for components at the 0.1‐1.0 % level can be obtained, and sometimes trace constituents can also be detected. Subsequently, the identity of the components can be determined by comparison to spectral libraries. Finally, the reconstructed pure component spectra can be further used to obtain the spatial distribution of the constituents present in the sample. Copyright © 2012 John Wiley & Sons, Ltd.
Thinking outside the classical chain reaction box of lipid oxidation
Authors: Schaich Karen M. 
Published: March 2012

AbstractFor the past few decades, research on lipid oxidation mechanisms has been rather stagnant due to a pervasive attitude that reactions of lipid oxidation were well understood, so only tailoring applications of basic knowledge to stabilize individual systems was needed. This simplistic approach worked during the low fat/no fat era because there was little lipid substrate to oxidize. However, with current reformulation of foods with polyunsaturated fatty acids for health, preventing oxidation of these essential fatty acids presents major challenges that cannot always be solved with traditional thinking. Because critical gaps in our understanding prevent moving forward with improved processing, formulations, and packaging to provide high‐PUFA lipid foods that remain shelf‐stable beyond a few months, details about oxidation mechanisms that were previously considered irrelevant have now become critically important. Considered here are alternate reaction pathways that compete with classical hydrogen abstraction and must be integrated into the overall free radical chain to more accurately account for kinetics and products of lipid oxidation.
Alternatives to tropical fats based on high‐stearic sunflower oils
Authors: Garcés Rafael , Martínez‐Force Enrique , Salas Joaquín J. , Bootello Miguel A. 
Published: March 2012

AbstractNew high‐oleic high‐stearic sunflower lines produce oils that could be a source of disaturated triacylglycerol alternatives to tropical fats. These oils can be fractionated to produce stearins enriched in stearic acid with physical properties similar to cocoa butter and other confectionary fats without hydrogenation or transesterification. These fats can be produced in temperate countries from a well‐established crop like sunflower, and represent a healthy source of saturated fatty acids.
The Effects of Nanofiber Topography on Astrocyte Behavior and Gene Silencing Efficiency
Authors: Cao Haoqing , Marcy Guillaume , Goh Eyleen Lay Keow , Wang Feng , Wang Jun , Chew Sing Yian 
Published: March 2012

AbstractAstrocyte‐nanofiber interactions are studied by culturing primary rat cortical astrocytes on poly[caprolactone‐co‐(ethyl ethylene phosphate)] electrospun nanofibers and solvent‐cast films (two‐dimensional control). The results indicate that nanofiber topography significantly suppresses astrocyte proliferation and enhances apoptosis, without altering cellular activation as compared to films. Moreover, nanofiber topography enhances gene‐silencing efficiency in astrocytes. The results suggest that nanofibers may serve as potential substrates for nerve regeneration by suppressing astrocyte growth and may further facilitate the use of gene‐silencing to enhance CNS regeneration.magnified image
Imaging measures predict progression in progressive supranuclear palsy
Authors: Whitwell Jennifer L. , Xu Jia , Mandrekar Jay , Gunter Jeffrey L. , Jack Clifford R. , Josephs Keith A. 
Published: March 2012

AbstractObjective:The aim of this work was to determine whether the progressive supranuclear palsy rating scale, a measure of disease severity, is associated with neuroanatomical changes in progressive supranuclear palsy.Methods:We recruited 22 subjects with probable progressive supranuclear palsy who completed the progressive supranuclear palsy rating scale at 2 time points. All subjects had magnetic resonance imaging and diffusion tensor imaging. Associations were assessed between whole brain volume, ventricular volume, midbrain area and superior cerebellar peduncle fractional anisotropy, and baseline/change of the progressive supranuclear palsy rating scale.Results:Superior cerebellar peduncle fractional anisotropy correlated with the total score and gait/midline subscore of the progressive supranuclear palsy rating scale. Whole brain volume, midbrain area, and disease duration predicted subsequent change over time in the gait/midline subscore of the progressive supranuclear palsy rating scale.Conclusions:Imaging measures could be useful to help predict clinical progression in subjects with progressive supranuclear palsy. © 2012 Movement Disorder Society
A CPW FED planar monopole antenna with modified H shaped slot for WLAN/Wi‐MAX application
Authors: Ghatak Rowdra , Goswami C. , Mishra R. K. , Poddar D. R. 
Published: March 2012

AbstractA planar monopole antenna with dual wideband resonance characteristics is presented that simultaneously satisfies wireless local area network (802.11 a/b) and Wi‐MAX applications. The proposed antenna structure consists of a rectangular monopole beveled at the base with a modified H shaped slot and occupies an area of 32 × 51.5 mm2. The measured realized gain is around 2.0 dBi in the lower band (2.35–2.80 GHz) and about 3.8 dBi in the upper band (3.28–5.95 GHz). © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:1296–1301, 2012
A compact UWB slot antenna with signal rejection in 5–6 GHz band
Authors: Subbarao Akkala , Raghavan S. 
Published: March 2012

AbstractIn this article, a novel planar slot antenna is presented with band‐notched characteristic for ultra wideband applications.The antenna is fed by coplanar wave guide. A “C” shaped slot etched on the radiating patch to avoid interference from WLAN and HYPERLAN/2 bands. The antenna has measured bandwidth ranging from 3 to 10.6 GHz for VSWR ≤ 2. The antenna has omni directional radiation pattern in H‐plane and bi‐directional in E‐plane. The group delay of antenna is less than 1 ns except in the notched band. Good agreement is found between measured and simulated results. The antenna can be easily integrated with microwave circuitry and is a good for UWB applications. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:1292–1296, 2012
A triple gain mode digitally controlled amplifier in cmos process
Authors: Park Bonghyuk , Jung Jaeho 
Published: March 2012

AbstractA high dynamic range, wide‐band triple gain mode amplifier has been developed using CMOS 0.18‐μm technology.The proposed low‐noise amplifier (LNA) consists of feedback architecture in the first stage and cascode topology in the second stage. The frequency response of 2.0–3.5 GHz is demonstrated in this work. In high‐gain mode, measured results show a noise figure (NF) of 2.6–2.9 dB, gain of 25.4–28.2 dB, and input P1dB of −19.0 dBm with 10.9 mA DC current. In mid‐gain mode, the LNA achieves 4.2–6.5 dB NF and 21.3–22.5 dB gain with 11.0 mA current consumption. In low‐gain mode, 6.7–8.5 dB NF, 10.2–12.5 dB gain, and −10 dBm input P1dB with 11.0 mA current are realized. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:1263–1266, 2012
Hybrid integration of 4‐W and 8‐W S‐band GaN power amplifiers on a selectively anodized aluminum substrate
Authors: Kim Dong‐Wook 
Published: March 2012

AbstractHybrid integration of 4‐W and 8‐W GaN power amplifiers operating in 2–3 GHz is attempted on a selectively anodized aluminum substrate. Discrete GaN high electron mobility transistors are inserted in selectively etched pockets of the aluminum metal substrate and all passive matching circuits are fabricated on the anodized aluminum oxide layer of the aluminum substrate. The 4‐W power amplifier shows a flat gain of 20–23 dB in 0.7–4.5 GHz, a maximum output power of 37.5 dBm at 2.5 GHz, and output power variation of ±0.35 dB in 2–3 GHz. The 8‐W power amplifier also shows a maximum output power of 39.6 dBm at 2.5 GHz and output power variation of ±0.5 dB in 2–3 GHz. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:1261–1263, 2012
Investigation of spatial filters at microwave frequencies: Application for antenna directivity enhancement
Authors: Yahiaoui Riad , Burokur Shah Nawaz , Vigneras Valérie , de Lustrac André , Mounaix Patrick 
Published: March 2012

AbstractWe report the theoretical and experimental investigations of a frequency selective surface (FSS) operating in the microwave regime. Numerical simulations using finite element method and reflection–transmission microwave measurements have been performed and a good quantitative agreement has been observed. Angular robustness is also demonstrated which makes it promising in telecommunication applications. The potential application of the FSS as a partially reflecting surface (PRS) reflector Fabry–Pérot (FP) cavity is proposed and evaluated. Measured return losses and radiations patterns done in an anechoic chamber agree very well with the simulated ones. A high directivity of 20 dBi is observed for the FP cavity. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:1327–1332, 2012
Noncontact detection of the location of buried conductive grids with pulsed THz wave
Authors: Chang Yun‐ching , Gao Yaohui , Wang Chao , Yao Jimmy , Cheng Jiping , Yin Stuart , Ruffin Paul , Brantley Christina , Edwards Eugene , Luo Claire 
Published: March 2012

AbstractIn this article, a new method for precisely measuring the location of buried conductive grid based on time‐domain pulsed terahertz (THz) reflectometry is presented. The location of the buried conductive grid is determined by the time difference of reflected THz pulses between the front surface and the buried conductive grid. Since the wavelength of THz wave is usually larger than the roughness of unpolished optical surface and the period of conductive grid, the proposed method is insensitive to the surface condition. Furthermore, this is a non‐contact and real‐time measurement technique. The experimental result indicates that the accuracy of the measurement is at least on the order of 10 μm. Such kind of technique will be very helpful for enhancing the quality and reduce the cost of fabricating electro‐optic windows with embedded conductive grids and/or wires. It can also be beneficial for some other applications. For example, since there are often embedded conductive wires in explosive devices, it can be used to detect the explosive devices because the proposed method can remotely determine the dimensions and locations of conductive wires embedded in dielectric enclosures. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:1135–1138, 2012
Miniaturization cantor set fractal ultrawideband antenna with a notch band characteristic
Authors: Li Yingsong , Yang Xiaodong , Liu Chengyuan , Jiang Tao 
Published: March 2012

AbstractA CPW‐fed wide slot ultrawideband antenna with notch band characteristic is presented numerically and experimentally. The radiation patch is implemented using Cantor set fractal technology. By setting two symmetrical triangular tapered corners at the bottom of the wide slot, the impedance bandwidth of the proposed antenna can be enhanced. In addition, a T‐shaped tuning stub suspends at the top of the wide slot to achieve the notch band characteristic. Experimental and numerical results show that the proposed antenna, with compact size of 26 × 21 mm2, has an impedance bandwidth range from 2.8 to 11 GHz for voltage standing‐wave ratio less than 2, except the rejected band 5–6.3 GHz. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:1227–1230, 2012