bluuebunny/crossref_metadata_2025_split huggingface dataset:
DivIVA controls the dynamics of septum splitting and cell elongation inStreptococcus pneumoniae
Authors: Trouve Jennyfer , Zapun André , Bellard Laure , Juillot Dimitri , Pelletier Anais , Freton Celine , Baudoin Morgane , Carballido-Lopez Rut , Campo Nathalie , Wong Yung-Sing , Grangeasse Christophe , Morlot Cecile 
Published: May 2024

AbstractBacterial shape and division rely on the dynamics of cell wall assembly, which involves regulated synthesis and cleavage of the peptidoglycan. In ovococci, these processes are coordinated in an annular mid-cell region with nanometric dimensions. More precisely, the cross-wall that is synthesized by the divisome is split to generate lateral wall, whose expansion is insured by insertion of so-called peripheral peptidoglycan by the elongasome. Septum cleavage and peripheral peptidoglycan synthesis are thus crucial remodeling events for ovococcal cell division and elongation. The structural DivIVA protein has long been known as a major regulator of these processes but its mode of action remains unknown. Here, we integrate click chemistry-based peptidoglycan labeling, direct stochastic optical reconstruction microscopy and in silico modeling, as well as epifluorescence and stimulated emission depletion microscopy to investigate the role of DivIVA inStreptococcus pneumoniaecell morphogenesis. Our work reveals two distinct phases of peptidoglycan remodeling along the cell cycle, that are differentially controlled by DivIVA. In particular, we show that DivIVA ensures homogeneous septum cleavage and peripheral peptidoglycan synthesis around the division site, and their maintenance throughout the cell cycle. Our data additionally suggest that DivIVA impacts the contribution of the elongasome and class A PBPs to cell elongation. We also report the position of DivIVA on either side of the septum, consistent with its known affinity for negatively curved membranes. Finally, we take the opportunity provided by these new observations to propose hypotheses for the mechanism of action of this key morphogenetic protein.ImportanceThis study sheds light on fundamental processes governing bacterial cell growth and division, using integrated click chemistry, advanced microscopy and computational modeling approaches. More precisely, it addresses mechanisms involved in the regulation of cell wall synthesis and remodeling inStreptococcus pneumoniae. This bacterium belongs to the morphological group of ovococci, which includes many human pathogens, such as streptococci and enterococci. In this study, we have dissected the function of DivIVA, which is a structural protein involved in cell division, cell morphogenesis and chromosome partitioning in Gram-positive bacteria. This work unveils the role of DivIVA in the orchestration of cell division and elongation along the pneumococcal cell cycle. It not only helps understanding how ovoid bacteria proliferate, but also offers an opportunity to consider how DivIVA might serve as a scaffold and sensor for particular membrane regions, and thus be involved in various processes associated with the cell cycle.
Structure and mechanism of biosynthesis ofStreptococcus mutanscell wall polysaccharide
Authors: Rush Jeffrey S. , Zamakhaeva Svetlana , Murner Nicholas R. , Deng Pan , Morris Andrew J. , Kenner Cameron W. , Black Ian , Heiss Christian , Azadi Parastoo , Korotkov Konstantin V. , Widmalm Göran , Korotkova Natalia 
Published: May 2024

AbstractStreptococcus mutans,the causative agent of human dental caries, expresses a cell wall attached Serotypec-specific Carbohydrate (SCC) that is critical for cell viability. SCC consists of a repeating →3)α-Rha(1→2)α-Rha(1→ polyrhamnose backbone, with glucose (Glc) side-chains and glycerol phosphate (GroP) decorations. This study reveals that SCC has one major and two minor Glc modifications. The major Glc modification, α-Glc, attached to position 2 of 3-rhamnose, is installed by SccN and SccM glycosyltransferases and is the site of the GroP addition. The minor Glc modifications are β-Glc linked to position 4 of 3-rhamnose installed by SccP and SccQ glycosyltransferases, and α-Glc attached to position 4 of 2-rhamnose installed by SccN working in tandem with an unknown enzyme. Both the major and the minor β-Glc modifications control bacterial morphology, but only the GroP and major Glc modifications are critical for biofilm formation.
Intraplacental injection of human iPSC-derived PDX1+ pancreatic progenitors prolongs Pdx1-deficient mice survival
Authors: Wakimoto Arata , Jeon Hyojung , Shahri Zeynab Javanfekr , Hayashi Takuto , Liao Ching-Wei , Gogoleva Natalia , Suchy Fabian , Noda Atsushi , An Yuri , Nakauchi Hiromitsu , Hayashi Yohei , Hamada Michito , Takahashi Satoru 
Published: May 2024

SummaryInterspecies chimeras comprising human tissues have potential for use in disease modeling and regenerative medicine. Here, we successfully transplanted human induced pluripotent stem cell (iPSC)-derived PDX1+ pancreatic progenitor cells into Pdx1-deficient mouse embryos via intraplacental injection. The engrafted human cells predominantly localized to the duodenum, produced insulin, and extended the lifespan ofPdx1-/-mice by up to 10 days after birth. Transcriptomic analyses confirmed human pancreatic gene expression in human cells engrafted into the mouse duodenum. Our findings demonstrate the feasibility of generating interspecies chimeras with functional human pancreatic cells throughin uterotransplantation of lineage-committed progenitors. This approach circumvents developmental barriers while minimizing ethical concerns associated with PSCs. However, the incomplete rescue of thePdx1-/-phenotype highlights the need for further research to enhance human cell engraftment and tissue integration. Overall, this study provides a foundation for developing human-animal chimera models for studying human development and regenerative therapies.
Vacuum chambers for Swiss Light Source arcs
Authors: Ganter R. , Braschoss P. , Braun H.-H. , Buchmann J. , Citterio A. , Dehler M. , Gaiffi N. , Kirchgeorg N. , Magjar M. , Rosenberg C. , Stephan D. , Schulz L. , Sieber R. , Wang X. , Zandonella A. 
Published: May 2024

The vacuum chambers for diffraction limited storage ring differ from the previous storage rings generation in three main aspects: the cross-section dimension, which is divided by a factor of 2 or more to fit smaller magnet apertures; the material, which includes much more copper for heat dissipation and to limit resistive wakefields; the coating of the inner surface with a nonevaporable getter (NEG) to ensure good pumping level despite low conductance. This paper gives a detailed description of the vacuum chambers’ design used in the arc section of SLS 2.0, starting with conceptual design choices based among others on synchrotron radiation heat and wakefield considerations. The particular case of the main bending magnet vacuum chamber is explained in detail from design to manufacturing, including the development of an appropriate NEG coating procedure. Finally, the overall assembly of a 17 m long arc, its activation to reach pressure in the 10−11  mbar range followed by transport and installation into the magnets is presented. This validates the choice of activation for an arc vessel made out of copper, with wall thicknesses as small as 1 mm and with less than 0.5 mm clearance to magnet poles.

Published by the American Physical Society
2024
Reducing linguistic profiling of individuals with communication disabilities to enhance careers: avoiding assumptions based on speech, language and communication mode
Authors: Caldwell Nicolette Sammarco , Holyfield Christine , Lorah Elizabeth , Rackensperger Tracy 
Published: May 2024

PurposeThe paper aims to support employers in avoiding linguistic profiling of individuals with communication disabilities; thereby reducing their exclusion from the workforce. It provides employers and others in the workplace with examples of speech, language and communication differences, common false stereotypes and assumptions, and strategies to prevent and address the negative effects of linguistic profiling.Design/methodology/approachThe paper offers a general overview of linguistic profiling as it relates to individuals with communication disabilities. Pertinent examples and findings from previous literature are included to illustrate linguistic profiling across speech, language and mode of communication, and to provide recommendations for inclusive workplace practices to ensure that career success is accessible to individuals with communication disabilities.FindingsLinguistic profiling is a barrier to successful and inclusive employment for individuals with communication disabilities. Education, training and the use of inclusive practices can reduce linguistic profiling of individuals with communication disabilities in the workplace.Originality/valueThough linguistic profiling used to make inferences of social constructs such as race and gender has long been discussed, little discussion surrounds individuals with communication disabilities and the impact it has in their lives and careers. This paper uniquely highlights communication disability in the linguistic profiling discussion so that organizations can be more aware of the impact and the need to create supportive and inclusive workplace environments and in turn reduce discrimination and increase diversity.
Antecedents and outcomes of firm internet-interactive capability in interfirm relationships
Authors: Feng Chao , Ding Shirui , Chen Hui , Zhang Yue 
Published: May 2024

Purpose
This study aims to explore whether and how the two potential antecedents (i.e. relationship quality at the dyadic level and network density at the network level) affect firms’ internet-interactive capability (FIIC), referring to the capability of a specific firm to communicate and interact with the relevant partner firms on the basis of internet-interactive technologies in the internet environment and, at the same time, the following influence of FIIC on collaborative activities (i.e. joint planning and joint problem-solving).

Design/methodology/approach
This study designed a questionnaire and collected data on-site from 400 manufacturers. SmartPLS is used to validate the research model.

Findings
The results suggest that the dyadic relationship quality and network density of the partner group are both positively connected with a firm’s FIIC. Besides, FIIC is positively related to collaborative activities with its partners.

Research limitations/implications
Given the nature of our data (i.e. cross-sectional), the authors can collect longitudinal or experimental data to retest the hypotheses.

Practical implications
This study gives certain guidance for firms to be aware of the factors that motivate FIIC and use their FIIC to influence their employees’ collaborative activities in their relationships with partners, thereby promoting cooperation performance.

Originality/value
This study attempts to extend the resource-based theory based on the logic of motivation-capability by exploring the potential antecedents of FIIC and makes contributions to the current studies on the antecedents of FIIC, which provides actionable insights for firms to play the role of FIIC in interfirm interactions.
Developing a global B2B strategy for products and services management: practical guidance from the field
Authors: Mora Cortez Roberto , Lecaros Roberto 
Published: May 2024

Purpose
This study aims to examine the challenges of developing a global business-to-business (B2B) offering from an emerging economy (Chile) during an internationalization endeavor. This study analyzes the development and management of an offering evolving from local to global.

Design/methodology/approach
The data were collected via an in-depth interview (105 min).

Findings
The interviewee presented a mixed of interesting marketing and/or marketing-related decisions (e.g. marketing–sales interface and marketing capabilities development) that support the internationalization of a firm and consequently its offering.

Research limitations/implications
The data represent a practical, ongoing rationalization of the events perceived by a manager. New theory can emerge from further exploring identified research opportunities.

Practical implications
The findings suggest that an emerging economy firm can gradually transition toward a multinational condition by coordinating a series of marketing and/or marketing related decisions, where the CMO (i.e. maximum marketing authority in the organization) plays an essential role.

Originality/value
This study brings the voice of practitioners to an academic environment and serves as an innovative effort to analyze B2B practitioners’ reality from a scholarly perspective. To the best of the authors’ knowledge, this study is the first one of the new series “Practitioners Insights” in the Journal of Business and Industrial Marketing.
The role of green bonds in reducing CO2 emissions: a case of developing countries
Authors: Arshad Ameena , Parveen Shagufta , Mir Faisal Nawaz 
Published: May 2024

PurposeThe global economy is growing very fast, and it is also facing environmental challenges. Due to increased economic activities, global warming is rising as a result of greenhouse gas emissions. Concepts like green finance and green investments are emerging to battle climate issues. The present study empirically examines the impact of green bonds on carbon dioxide (CO2) emissions in developing countries, as these countries are producing 63% of CO2 emissions around the globe.Design/methodology/approachTo check this impact, pooled ordinary least squares (OLS), fixed effect and generalized method of moments (GMM) techniques are applied using the annual data of 65 developing countries from 2008 through 2021.FindingsThe results indicate that the overall effect of green bonds on CO2 emissions is negative, as more issuance of green bonds reduces CO2 emissions, confirming results from the existing empirical literature. The study found that more foreign direct investment (FDI) and urbanization lead to more CO2 emissions, while increase in trade openness helps reduce CO2 emissions. It was found that promoting green bonds will help to promote environmentally friendly projects that will help to reduce CO2 emissions. Rapid urbanization has led to more energy demand for various industries like manufacturing, transportation and residential sectors, which leads to more CO2 emissions.Practical implicationsThe policymakers in these countries should make policies that help in reducing carbon emission by increasing green bonds and FDI in supporting projects that are environmentally friendly. Therefore, to mitigate such current and future issues, policymakers in developing countries need to give serious attention to this area to fulfill sustainable development goals.Originality/valueThis study presents a pioneering examination of green bonds and CO2 emissions in 65 lower- and middle-income countries (developing countries). We have tried to cover all developing countries that are causing more greenhouse gas emissions and need to shift to green finance strategies. It will be a contribution to the body of knowledge regarding the role of green bonds in reducing CO2 emissions. The present study will help in assessing the importance of green bonds in bringing low-carbon economies.
Study on barrier properties, biodegradability and antimicrobial activity of linear low density polyethylene/starch blends
Authors: Shafik Emad S. , Faheem Mena , El-Sheikh Marwa , Mohamed Amira Abdalla Abdelshafy , Soliman Seham Samir 
Published: May 2024

Purpose
The present work aims to prepare biocomposites blend based on linear low density polyethylene/ starch without using harmful chemicals to improve the adhesion between two phases. Also, the efficiency of essential oils as green plasticizers and natural antimicrobial agents were evaluated.

Design/methodology/approach
Barrier properties and biodegradation behavior of linear low density polyethylene/starch (LLDPE/starch) blends plasticized with different essential oils including moringa oleifera and castor oils wereassessed as a comparison with traditional plasticizer such as glycerol. Biodegradation behavior forLLDPE/starch blends was monitored by soil burial test. The composted samples were recovered then washed followed by drying, and weighting samples after 30, 60, and 90 days to assess the change in weight loss. Also, mechanical properties including retention values of tensile strength and elongation at break were measured before and after composting. Furthermore, scanning electron microscope (SEM) was used to evaluate the change in the morphology of the polymeric blends. In addition to, the antimicrobial activity of plasticized LLDPE/starch blends films was evaluated using a standard plate counting technique.

Findings
The results illustrate that the water vapor transition rate increases from 2.5 g m−2 24 h−1 for LLDPE/5starch to 4.21 g m−2 24 h−1 and 4.43 g m−2 24 h−1 for castor and moringa oleifera respectively. Also, the retained tensile strength values of all blends decrease gradually with increasing composting period. Unplasticized LLDPE/5starch showed highest tensile strength retention of 91.6% compared to the other blends that were 89.61, 88.49 and 86.91 for the plasticized LLDPE/5starch with glycerol, castor and M. oleifera oils respectively. As well as, the presence of essential oils in LLDPE/ starch blends increase the inhibition growth of escherichia coli, candida albicans and staphylococcus aureus.

Originality/value
The objective of this work is to develop cost-effective and environmentally-friendly methods for preparing biodegradable polymers suitable for packaging applications.
Universal Jurisdiction: Law out of Context
Authors: Hovell Devika , Malagodi Mara 
Published: May 2024

Universal jurisdiction enables the prosecution of international crimes by domestic courts in the absence of any nexus between the prosecuting state and the crime charged. While the temptation is for domestic judges to proceed with ‘business as usual’ in the conduct of such trials, difficulties in the practice of universal jurisdiction reflect the importance of developing a better understanding of the distinctive communities, interests, crimes and cultures these trials are intended to serve. The exercise of universal jurisdiction is commonly regarded as a form of domestic jurisdiction exercised pursuant to a sovereign right under international law. This article invites a re‐conceptualisation of the concept of universal jurisdiction, explaining that it is not a form of domestic jurisdiction acquired based on sovereign nexus between the crime charged and the prosecuting state. Instead, it should be recognised as a form of decentralised ‘international jurisdiction’, exercised as part of a state's contribution to the enforcement of international criminal law. This re‐conceptualisation has implications for the way in which domestic courts engage with many of the challenges facing universal jurisdiction trials, including problems of community, case selection, proof and translation.
Oral misoprostol alone, compared with oral misoprostol followed by oxytocin, in women induced for hypertension of pregnancy: A multicentre randomised trial
Authors: Mundle Shuchita , Lightly Kate , Durocher Jill , Bracken Hillary , Tadas Moushmi , Parvekar Seema , Shivkumar Poonam Varma , Faragher Brian , Easterling Thomas , Leigh Simon , Turner Mark , Alfirevic Zarko , Winikoff Beverly , Weeks Andrew D. 
Published: May 2024

AbstractObjectiveTo assess whether, in those requiring continuing uterine stimulation after cervical ripening with oral misoprostol and membrane rupture, augmentation with low‐dose oral misoprostol is superior to intravenous oxytocin.DesignOpen‐label, superiority randomised trial.SettingGovernment hospitals in India.PopulationWomen who were induced for hypertensive disease in pregnancy and had undergone cervical ripening with oral misoprostol, but required continuing stimulation after artificial membrane rupture.MethodsParticipants received misoprostol (25 micrograms, orally, 2‐hourly) or titrated oxytocin through an infusion pump. All women had one‐to‐one care; fetal monitoring was conducted using a mixture of intermittent and continuous electronic fetal monitoring.Main outcome measuresCaesarean birth.ResultsA total of 520 women were randomised and the baseline characteristics were comparable between the groups. The caesarean section rate was not reduced with the use of misoprostol (misoprostol, 84/260, 32.3%, vs oxytocin, 71/260, 27.3%; aOR 1.23; 95% CI 0.81–1.85; P = 0.33). The interval from randomisation to birth was somewhat longer with misoprostol (225 min, 207–244 min, vs 194 min, 179–210 min; aOR 1.137; 95% CI 1.023–1.264; P = 0.017). There were no cases of hyperstimulation in either arm. The rates of fetal heart rate abnormalities and maternal side effects were similar. Fewer babies in the misoprostol arm were admitted to the special care unit (10 vs 21 in the oxytocin group; aOR 0.463; 95% CI 0.203–1.058; P = 0.068) and there were no neonatal deaths in the misoprostol group, compared with three neonatal deaths in the oxytocin arm. Women's acceptability ratings were high in both study groups.ConclusionsFollowing cervical preparation with oral misoprostol and membrane rupture, the use of continuing oral misoprostol for augmentation did not significantly reduce caesarean rates, compared with the use of oxytocin. There were no hyperstimulation or significant adverse events in either arm of the trial.
Dual‐role regulator of a novel miR‐3040 in photoperiod‐mediated wing dimorphism and wing development in green peach aphid
Authors: Zhang Yun‐Hui , Qian Xin , Zong Xin , An Shi‐Heng , Yan Shuo , Shen Jie 
Published: May 2024

AbstractWing dimorphism is regarded as an important phenotypic plasticity involved in the migration and reproduction of aphids. However, the signal transduction and regulatory mechanism of wing dimorphism in aphids are still unclear. Herein, the optimal environmental conditions were first explored for inducing winged offspring of green peach aphid, and the short photoperiod was the most important environmental cue to regulate wing dimorphism. Compared to 16 L:8 D photoperiod, the proportion of winged offspring increased to 90% under 8 L:16 D photoperiod. Subsequently, 5 differentially expressed microRNAs (miRNAs) in aphids treated with long and short photoperiods were identified using small RNA sequencing, and a novel miR‐3040 was identified as a vital miRNA involved in photoperiod‐mediated wing dimorphism. More specifically, the inhibition of miR‐3040 expression could reduce the proportion of winged offspring induced by short photoperiod, whereas its activation increased the proportion of winged offspring under long photoperiod. Meanwhile, the expression level of miR‐3040 in winged aphids was about 2.5 times that of wingless aphids, and the activation or inhibition of miR‐3040 expression could cause wing deformity, revealing the dual‐role regulator of miR‐3040 in wing dimorphism and wing development. In summary, the current study identified the key environmental cue for wing dimorphism in green peach aphid, and the first to demonstrate the dual‐role regulator of miR‐3040 in photoperiod‐mediated wing dimorphism and wing development.