bluuebunny/crossref_metadata_2025_split huggingface dataset:
Plasma pentosidine: a potential biomarker in the management of multiple sclerosis
Authors: Sternberg Zohara , Hennies Cassandra , Sternberg Daniel , Bistulfi Gaia L , Kazim Latif , Benedict Ralph HB , Chadha Kailash , Leung Christopher , Weinstock-Guttman Bianca , Munschauer Frederick 
Published: October 2010

Background: The chronic inflammation associated with multiple sclerosis (MS) may lead to the upregulation of pentosidine. Objectives: This cross-sectional study compares plasma pentosidine levels among healthy controls (HCs) and patients with MS at different disease stages. The study also determines pentosidine's usefulness as a biomarker of MS disease activity and/or severity via its correlation with a number of indicators of MS disease. Methods: Pentosidine levels were analyzed in 98 MS patients and 43 HCs using reverse-phase high-pressure liquid chromatography with fluorescence detection. Results: Plasma pentosidine levels were significantly higher in MS patients when compared with HCs ( p = 0.02). Patients on disease-modifying therapies (DMTs) had lower plasma pentosidine levels when compared with DMT-naïve patients ( p =  0.01). Pentosidine plasma levels correlated with indicators of MS disease severity, including Extended Disability Status Scale ( p = 0.03), MS Severity Scale ( p = 0.01), and MS Functional Composite ( p = 0.03). No correlation between pentosidine levels and age, rate of clinical relapse, and disease duration was observed. Conclusions: Our results suggest that pentosidine could be a novel, inflammatory biomarker in MS clinical practice. Longitudinal studies are warranted to determine any causal relationship between changes in plasma pentosidine levels and MS disease pathology. These studies may pave the way for use of advanced glycation end product (AGE) inhibitors and AGE-breaking agents as new therapeutic modalities in MS.
Prognostic value of cerebrospinal fluid analysis at the time of a first demyelinating event
Authors: Gout Olivier , Bouchareine Annie , Moulignier Antoine , Deschamps Romain , Papeix Caroline , Gorochov Guy , Héran Françoise , Bastuji-Garin Sylvie 
Published: October 2010

Background and Objective: This study aimed to assess the value of cerebrospinal fluid (CSF) findings for predicting conversion to clinically definite multiple sclerosis (CDMS). Methods: From a database of 447 patients with a first demyelinating event, the records of 208 patients less than 51 years old who had baseline magnetic resonance imaging (MRI) and CSF examinations and a follow-up of at least 1 year were included. A multivariable Cox model was used to assess the short-term risk of CDMS according to baseline CSF findings after adjustment for prognostic factors (including brain MRI) and to provide a simple classification for predicting CDMS. Results: During a median follow-up of 3.5 years, 141 (67.8%) patients converted to CDMS. In multivariate analysis, younger age (hazard ratio [HR]: 1.44 [95% CI 1.02–2.01]), spatial dissemination on brain MRI (HR: 2.07 [95% CI 1.47–2.91]) and more than 4 WBC/mm3 in CSF (HR: 1.44 [95% CI 1.03–2.02]) were independently associated with CDMS. The Cox score obtained from these three predictors enabled patients to be divided into three groups with significant increased risks of CDMS at 1, 2 and 3 years; groups were classified as high-risk (64.7%, 77.4%, 96.1%), intermediate-risk (33.3%, 51.5%, 61.5%), and low-risk (11.1%, 18.3%, 40.3%). Conclusions: Age at onset, spatial dissemination on brain MRI and CSF white blood cell count are independently associated with short-term conversion to CDMS. The three proposed risk group classifications could be a useful tool to select patients for early therapeutic intervention.
Infusion of HLA-mismatched peripheral blood stem cells improves the outcome of chemotherapy for acute myeloid leukemia in elderly patients
Authors: Guo Mei , Hu Kai-Xun , Yu Chang-Lin , Sun Qi-Yun , Qiao Jian-Hui , Wang Dan-Hong , Liu Guang-Xian , Sun Wan-Jun , Wei Li , Sun Xue-Dong , Huang Ya-Jing , Qiao Jun-Xiao , Dong Zheng , Ai Hui-Sheng 
Published: October 2010

Abstract
Treatment outcome of acute myeloid leukemia (AML) in elderly patients remains unsatisfactory. It has been shown that the infusion of granulocyte colony-stimulating factor–mobilized donor peripheral blood stem cells (G-PBSCs) can enhance graft-versus-leukemia effects and speed hematopoietic recovery. Fifty-eight AML patients aged 60-88 years were randomly assigned to receive induction chemotherapy with cytarabine and mitoxantrone (control group; n = 28) or it plus human leukocyte antigen–mismatched G-PBSCs (G-PBSC group; n = 30). Patients who achieved complete remission received another 2 cycles of postremission therapy with intermediate-dose cytarabine or it plus G-PBSCs. The complete remission rate was significantly higher in the G-PBSC group than in the control group (80.0% vs 42.8%; P = .006). The median recovery times of neutrophils and platelets were 11 days and 14.5 days, respectively, in the G-PBSC group and 16 days and 20 days, respectively, in the control group after chemotherapy. The 2-year probability of disease-free survival was significantly higher in the G-PBSC group than in the control group (38.9% vs 10.0%; P = .01). No graft-versus-host disease was observed in any patient. Persistent donor microchimerism was successfully detected in all of the 4 female patients. These results indicate that G-PBSCs in combination with conventional chemotherapy may provide a promising treatment method for AML in elderly patients.
The platelet P2Y12 receptor for adenosine diphosphate: congenital and drug-induced defects
Authors: Cattaneo Marco 
Published: October 2010

Abstract
P2Y12, the Gi-coupled platelet receptor for adenosine diphosphate (ADP), plays a central role in platelet function. Patients with congenital P2Y12 defects display a mild to moderate bleeding diathesis, characterized by mucocutaneous bleedings and excessive post-surgical and post-traumatic blood loss. Defects of P2Y12 should be suspected when ADP, even at high concentrations (≥ 10μM), is unable to induce full, irreversible platelet aggregation. Tests that evaluate the degree of inhibition of adenylyl cyclase by ADP should be used to confirm the diagnosis. Drugs that inhibit P2Y12 are potent antithrombotic drugs, attesting the central role played by P2Y12 in platelet thrombus formation. Clopidogrel, the most widely used drug that inhibits P2Y12, is effective both in monotherapy and in combination with acetylsalicylic acid. The most important drawback of clopidogrel is its inability to inhibit adequately P2Y12-dependent platelet function in approximately one-third of patients who are therefore not protected from major cardiovascular events. New drugs, such as prasugrel and ticagrelor, which effectively inhibit P2Y12 in the majority of patients, proved to be more efficacious than clopdidogrel in preventing major cardiovascular events. Although they increase the incidence of major bleedings, the net clinical benefit is in favor of the new P2Y12 inhibitors.
Development and validation of a flax (Linum usitatissimum L.) gene expression oligo microarray
Authors: Fenart Stéphane , Ndong Yves-Placide Assoumou , Duarte Jorge , Rivière Nathalie , Wilmer Jeroen , van Wuytswinkel Olivier , Lucau Anca , Cariou Emmanuelle , Neutelings Godfrey , Gutierrez Laurent , Chabbert Brigitte , Guillot Xavier , Tavernier Reynald , Hawkins Simon , Thomasset Brigitte 
Published: October 2010

AbstractBackgroundFlax (Linum usitatissimumL.) has been cultivated for around 9,000 years and is therefore one of the oldest cultivated species. Today, flax is still grown for its oil (oil-flax or linseed cultivars) and its cellulose-rich fibres (fibre-flax cultivars) used for high-value linen garments and composite materials. Despite the wide industrial use of flax-derived products, and our actual understanding of the regulation of both wood fibre production and oil biosynthesis more information must be acquired in both domains. Recent advances in genomics are now providing opportunities to improve our fundamental knowledge of these complex processes. In this paper we report the development and validation of a high-density oligo microarray platform dedicated to gene expression analyses in flax.ResultsNine different RNA samples obtained from flax inner- and outer-stems, seeds, leaves and roots were used to generate a collection of 1,066,481 ESTs by massive parallel pyrosequencing. Sequences were assembled into 59,626 unigenes and 48,021 sequences were selected for oligo design and high-density microarray (Nimblegen 385K) fabrication with eight, non-overlapping 25-mers oligos per unigene. 18 independent experiments were used to evaluate the hybridization quality, precision, specificity and accuracy and all results confirmed the high technical quality of our microarray platform. Cross-validation of microarray data was carried out using quantitative qRT-PCR. Nine target genes were selected on the basis of microarray results and reflected the whole range of fold change (both up-regulated and down-regulated genes in different samples). A statistically significant positive correlation was obtained comparing expression levels for each target gene across all biological replicates both in qRT-PCR and microarray results. Further experiments illustrated the capacity of our arrays to detect differential gene expression in a variety of flax tissues as well as between two contrasted flax varieties.ConclusionAll results suggest that our high-density flax oligo-microarray platform can be used as a very sensitive tool for analyzing gene expression in a large variety of tissues as well as in different cultivars. Moreover, this highly reliable platform can also be used for the quantification of mRNA transcriptional profiling in different flax tissues.
Psidium guvajava L Leaf Powder — A Potential Low-cost Biosorbent for the Removal of Cadmium(II) Ions from Wastewater
Authors: Rao K.S. , Anand S. , Venkateswarlu P. 
Published: October 2010

In the present investigation, an evaluation has been made of the adsorption of Cd(II) ions onto Psidium guvajava L leaf powder (PGL). Scanning electron micrographs of the powder as-prepared showed that PGL possessed an irregular texture consisting of globular and elongated grains. The porosity of the structure was also noted. After adsorption of Cd(II) ions, ca. 30% of the powdered grains on the resulting surface showed a pixel concentration of Cd(II) ions, indicating the adsorption of Cd(II) ions by selective grains. The FT-IR spectra of the as-prepared powder and the Cd(II) ion-loaded powder were similar but the absorption bands were shifted by 5–35 cm−1 after the adsorption process. The experimental parameters chosen for adsorption studies included time, pH, particle size, temperature, adsorbate, biosorbent, anions and Pb(II) ion concentrations. The kinetic data followed linear and non-linear pseudo-second-order kinetics. The adsorption of Cd(II) ions increased from zero to 60.39% as the pH of the aqueous solution was increased from 2 to 3.5, with a further increase in pH to 5.5 resulting in a marginal increase in the extent of adsorption to 63.7%. The isothermal data were well fitted by the Freundlich adsorption model. On the basis of the Langmuir isotherm model, the maximum loading capacity was calculated as 31.15 mg/g. The presence of chloride or sulphate anions had an adverse effect on the Cd(II) ion uptake by PGL. Lead(II) ions were adsorbed preferentially from binary Cd(II)/Pb(II) ion solutions, thereby decreasing the Cd(II) ion uptake. The thermodynamic parameters for the process were also estimated.
A nyitott, feszülésmentes, illetve feszülés mellett végzett lágyéksérvműtét hosszú távú eredményei – retrospektív vizsgálat
Authors: Baracs József , Huszár Orsolya , Gadácsi Melinda , Horváth Örs Péter , Wéber György 
Published: October 2010

Absztrakt
A szerzők ismertetik a 2004-ben a Pécsi Tudományegyetem Sebészeti Klinikáján lágyéksérv miatt operált összes beteg hosszú távú utánkövetési eredményeit. Az értékelés során a feszülésmentes és a feszülés mellett végzett, nyitott herniotomiákat vetjük össze a betegek kérdőívre adott válasza alapján. Eredmények: Eredményeinket 155 beteg adatainak elemzése alapján közöljük. Recidívaarány hálóval (H) 3,4% (4/116), háló nélkül (HN) 12,8% (5/39). Teljesen panaszmentes a betegek 83 (97/116 – H), illetve 89%-a (35/39 – HN). Jelentős krónikus fájdalom 1,7% (2/116 – H), illetve 7,7% (3/39 – HN). Korai aktivitás elérése 34% (39/116 – H), illetve 29% (11/39 – HN). Következtetés: Vizsgálatunk igazolta, hogy Lichtenstein-műtét után alacsonyabb a recidíva, azonban a krónikus fájdalom, illetve az aktivitás visszanyerésének tekintetében vizsgálatunk nem talált lényeges különbséget a feszülésmentes és a feszülés mellett végzett, nyitott herniotomia között.
Kedvezőbb műtéti eredmények „onlay” hálóval, mint „sublay” helyzetben beültetettel
Authors: Wéber György , Baracs József , Horváth Örs Péter 
Published: October 2010

AbsztraktBevezetés:A hasfal rekonstrukciójára számos műtéti megoldás ismert, az eredmények azonban meglehetősen szerények és ellentmondásosak. Vizsgálatunk célja a hasfali sérvek varrattal, illetve hálóbeültetéssel végzett műtéti kezelésének valamint az onlay, illetve sublay helyzetben beültetett hálók műtéti eredményeinek összehasonlítása.Anyag és módszerek:A 2002-ben kezdődött multicentrikus, prospektív, randomizált vizsgálatban az ország 20 sebészeti osztályán (lásd Függelék) 953 beteg hasfali rekonstrukcióját végeztük el. A betegeket, a sérv nagysága szerint két csoportba osztottuk: A csoport (n= 494): a sérvkapu nagysága 5–25 cm2(kis sérv) és B csoport (n= 459): a sérvkapu nagyobb, mint 25 cm2(nagy sérv). A randomizálásnak megfelelően az A csoportban a hasfal-rekonstrukció varrattal (n= 247), illetve hálóbeültetéssel (sublayn= 247) történt. A 25 cm2-nél nagyobb sérvek esetében (B csoport) hálóbeültetés történt, random módon sublay (n= 235), illetve onlay technikával (n= 224). Az utánkövetés öt évig tartott, és 2009 márciusában fejeződött be.Eredmények:Az ötéves utánkövetés során 219 beteget zártunk ki a vizsgálatból, így 734 beteg (a randomizált betegek 77%-a) adatait tudtuk elemezni. Az A csoport varrattal kezelt 184 betegénél 50 esetben (27%), míg a hálóval kezelt 180 betegénél 15 esetben (8%) találtunk recidív sérvet, a különbség szignifikáns (p< 0,001). A nagy sérvek eseté-ben (B csoport) a 189 sublay meshnél 38 esetben (20%), míg a 181 onlay meshnél 22 esetben (12%) találtunk recidív sérvet, a különbség ugyancsak szignifikáns (p< 0,05).Következtetések:A hálóbeültetés jobb, mint a varrattal történő hasfal-rekonstrukció. A 25 cm2-nél nagyobb sérvek esetében – a szakmai vélekedéssel ellentétben – az „onlay” hálóval szignifikánsan jobbak a műtéti eredmények, mint a „sublay” helyzetben beültetett hálóval.A randomizált vizsgálat ID száma NCT01018524 (www.ClinicalTrials.gov).
A lágyéktáji és hasfali sérvek laparoscopos műtétei – áttekintés
Authors: Bátorfi József 
Published: October 2010

Absztrakt
A közlemény áttekinti a laparoscopos sérvsebészet történetét, a lágyéktáji és ventralis sérvek laparoscopos megoldásait, technikáját, eredményeit. Az előnyök és hátrányok mérlegelése alapján megállapítja, hogy a laparoscopos sérvműtétek kiváló eredményekkel járnak a korai rehabilitáció, a közvetlen és távoli posztoperatív fájdalom, valamint az igen alacsony (kevesebb mint 1, illetve 5%) recidívaarány vonatkozásában. Gyakorlott sebész kezében méltán tekinthetők a sérvműtétek gold standardjének.
Parastomalis sérvek
Authors: Nagy Attila , Jánó Zoltán 
Published: October 2010

AbsztraktA parastomalis sérvek gyakorisága 35–50%. A rekonstrukciójukra használt módszerek (helyben hagyott stoma körüli hasfal-rekonstrukció, stomaáthelyezés és sérvkapuzárás, különböző módszerrel beültetett különböző típusú hálók) sem hoztak kielégítő eredményeket.Az endoscopos módszerek elkerülik a stoma körüli metszést, csökkentik a háló felülfertőződésének veszélyét. A sok próbálkozás ellenére a rekonstrukciók után az újabb recidívaarány még mindig 12–35% között maradt.A nem kielégítő eredmények alapján egyre erőteljesebben vetődik fel a stomakészítéssel egy időben beültetett hálók használata. Szerzők saját speciális, kétrétegű, hengeres összekötőelemmel kiegészített preventív hálóbeültetéssel képzett 17 stoma 5 éves utánvizsgálata során egyetlen parastomalis sérvet sem észleltek, összes szövődményük egy könnyen korrigálható bőrszintű strictura volt. Tapasztalataik alapján megfontolandónak tartják a preventív hálóbeültetést a véglegesnek szánt stoma készítése során.
A különböző szilikonbevonatú polipropilén hálók szöveti integrációja
Authors: Takács Ildikó , Horváth Szabolcs , Balatonyi Borbála , Jávor Szaniszló , Molnár Ágnes , Gáspár Sarolta , Hajós Rebeka , Meczker Ágnes , Lantos János , Rőth Erzsébet , Wéber György 
Published: October 2010

AbsztraktBevezetés/célkitűzés:A hasfali sérvek laparoscopos megoldásában a sebészi háló intraperitonealis pozícióban kerül beültetésre. Állatmodellekben és a humángyakorlatban egyaránt ígéretesek a kombinált, többrétegű hálók. Jelen kísérlet célja két eltérő szerkezetű, szilikonbevonatú polipropilén háló biológiai viselkedésének összehasonlítása volt.Anyag és módszerek:Új-zélandi fehér nyulak hasfalán egy 3 × 4 cm nagyságú, teljes vastagságú hasfaldefektust készítettünk, melyet összetett hálóval fedtünk. 20 állatba a felszínén lamináris szilikonréteggel bevont hálót (LSPP), a másik 20 nyúl esetében szilikonnal impregnált polipropilén filamentumból szőtt hálót (MSPP) ültettünk be. Az intraperitonealis adhaesioképződés mértékét és a hálók beépülését vizsgáltuk. Immunhisztokémiai módszerrel az osztódó sejtek jelenlétét és eloszlását az idegen anyag környezetében (Ki-67), az érújdonképződést (VEGF) és a kialakuló neoperitoneum-réteget (CK) vizsgáltuk, scanning elektronmikroszkóppal a halók felszínét tanulmányoztuk.Eredmények:Az intraperitonealis adhaesioképződés mértéke az első héten nem mutatott eltérést, míg a harmadik héten az LSPP szignifikánsan kevesebb kitapadást generált. A Ki-67 antitest jelölés szignifikánsan alacsonyabb aktivitású volt a makroporózus hálók csoportjában. Ugyanebben a csoportban a VEGF pozitivitás időarányosan nőtt, míg az LSPP esetében nem változott. A vékony neoperitoneum-réteg csak a makroporózus háló felszínén volt szövettanilag kimutatható (CK jelölés), az LSPP esetében hiányzott.Következtetések:A sebészi háló alapanyaga és textúrája is meghatározó szerepet játszik a befogadó szövetbe történő integrációban, mely arányos a kiváltott idegentest-reakcióval.
Applying the Chronic Care Model to Homeless Veterans: Effect of a Population Approach to Primary Care on Utilization and Clinical Outcomes
Authors: O'Toole Thomas P. , Buckel Lauren , Bourgault Claire , Blumen Jonathan , Redihan Stephen G. , Jiang Lan , Friedmann Peter 
Published: October 2010

Objectives. We compared a population-tailored approach to primary care for homeless veterans with a usual care approach. Methods. We conducted a retrospective prolective cohort study of homeless veterans enrolled in a population-tailored primary care clinic matched to a historical sample in general internal medicine clinics. Overall, 177 patients were enrolled: 79 in the Homeless-Oriented Primary Care Clinic and 98 in general internal medicine primary care. Results. Homeless-oriented primary care–enrolled patients had greater improvements in hypertension, diabetes, and lipid control, and primary care use was higher during the first 6 months (5.96 visits per person vs 1.63 for general internal medicine) but stabilized to comparable rates during the second 6 months (2.01 vs 1.31, respectively). Emergency department (ED) use was also higher (2.59 vs 1.89 visits), although with 40% lower odds for nonacute ED visits than for the general internal medicine group (95% confidence interval = 0.2, 0.8). Excluding substance abuse and mental health admissions, hospitalizations were reduced among the homeless veterans between the 2 periods (28.6% vs 10.8%; P < .01) compared with the general internal medicine group (48.2% vs 44.4%; P = .6; difference of differences, P < .01). Conclusions. Tailoring primary care to homeless veterans can decrease unnecessary ED use and medical admissions and improve chronic disease management.
Smoking Characteristics of Adults With Selected Lifetime Mental Illnesses: Results From the 2007 National Health Interview Survey
Authors: McClave Annette K. , McKnight-Eily Lela R. , Davis Shane P. , Dube Shanta R. 
Published: October 2010

Objectives. We estimated smoking prevalence, frequency, intensity, and cessation attempts among US adults with selected diagnosed lifetime mental illnesses. Methods. We used data from the 2007 National Health Interview Survey on 23 393 noninstitutionalized US adults to obtain age-adjusted estimates of smoking prevalence, frequency, intensity, and cessation attempts for adults screened as having serious psychological distress and persons self-reporting bipolar disorder, schizophrenia, attention deficit disorder or hyperactivity, dementia, or phobias or fears. Results. The age-adjusted smoking prevalence of adults with mental illness or serious psychological distress ranged from 34.3% (phobias or fears) to 59.1% (schizophrenia) compared with 18.3% of adults with no such illness. Smoking prevalence increased with the number of comorbid mental illnesses. Cessation attempts among persons with diagnosed mental illness or serious psychological distress were comparable to attempts among adults without mental illnesses or distress; however, lower quit ratios were observed among adults with these diagnoses, indicating lower success in quitting. Conclusions. The prevalence of current smoking was higher among persons with mental illnesses than among adults without mental illnesses. Our findings stress the need for prevention and cessation efforts targeting adults with mental illnesses.
Mental Health Promotion as a New Goal in Public Mental Health Care: A Randomized Controlled Trial of an Intervention Enhancing Psychological Flexibility
Authors: Fledderus Martine , Bohlmeijer Ernst T. , Smit Filip , Westerhof Gerben J. 
Published: October 2010

Objectives. We assessed whether an intervention based on acceptance and commitment therapy (ACT) and mindfulness was successful in promoting positive mental health by enhancing psychological flexibility.Methods. Participants were 93 adults with mild to moderate psychological distress. They were randomly assigned to the group intervention (n = 49) or to a waiting-list control group (n = 44). Participants completed measures before and after the intervention as well as 3 months later at follow-up to assess mental health in terms of emotional, psychological, and social well-being (Mental Health Continuum–Short Form) as well as psychological flexibility (i.e., acceptance of present experiences and value-based behavior, Acceptance and Action Questionnaire-II).Results. Regression analyses showed that compared with the participants on the waiting list, participants in the ACT and mindfulness intervention had greater emotional and psychological well-being after the intervention and also greater psychological flexibility at follow-up. Mediational analyses showed that the enhancement of psychological flexibility during the intervention mediated the effects of the intervention on positive mental health.Conclusions. The intervention is effective in improving positive mental health by stimulating skills of acceptance and value-based action.