bluuebunny/crossref_metadata_2025_split huggingface dataset:
Targeting BMAL1 reverses drug resistance of acute myeloid leukemia cells and promotes ferroptosis through HMGB1-GPX4 signaling pathway
Authors: Zheng Hong , Wu Ting , Lin Zhi , Wang Dan , Zhang Jing , Zeng Ting , Liu Leping , Shen Jie , Zhao Mingyi , Li Jia-Da , Yang Minghua 
Published: May 2024

Abstract
Purpose
Acute myeloid leukemia (AML) is a refractory hematologic malignancy that poses a serious threat to human health. Exploring alternative therapeutic strategies capable of inducing alternative modes of cell death, such as ferroptosis, holds great promise as a viable and effective intervention.

Methods
We analyzed online database data and collected clinical samples to verify the expression and function of BMAL1 in AML. We conducted experiments on AML cell proliferation, cell cycle, ferroptosis, and chemotherapy resistance by overexpressing/knocking down BMAL1 and using assays such as MDA detection and BODIPY 581/591 C11 staining. We validated the transcriptional regulation of HMGB1 by BMAL1 through ChIP assay, luciferase assay, RNA level detection, and western blotting. Finally, we confirmed the results of our cell experiments at the animal level.

Results
BMAL1 up-regulation is an observed phenomenon in AML patients. Furthermore, there existed a strong correlation between elevated levels of BMAL1 expression and inferior prognosis in individuals with AML. We found that knocking down BMAL1 inhibited AML cell growth by blocking the cell cycle. Conversely, overexpressing BMAL1 promoted AML cell proliferation. Moreover, our research results revealed that BMAL1 inhibited ferroptosis in AML cells through BMAL1-HMGB1-GPX4 pathway. Finally, knocking down BMAL1 can enhance the efficacy of certain first-line cancer therapeutic drugs, including venetoclax, dasatinib, and sorafenib.

Conclusion
Our research results suggest that BMAL1 plays a crucial regulatory role in AML cell proliferation, drug resistance, and ferroptosis. BMAL1 could be a potential important therapeutic target for AML.
Effects of concurrent chemoradiotherapy with or without Endostar on the regression of retropharyngeal lymph node and prognosis of patients with locally advanced nasopharyngeal carcinoma: a retrospective study
Authors: Song Jun-Mei , Mo Ning , Lv Yu-Qing , Huang Lu-Lu , Wen Ya-Jing , Liu Ting , Li Zhi-Ru , Wang Ren-Sheng , Zhang Ting-Ting 
Published: May 2024

Abstract
Background and Purpose
To investigate the effect of combining Endostar with concurrent chemoradiotherapy (ECCRT) compared to concurrent chemoradiotherapy (CCRT) on the regression rate of retropharyngeal lymph nodes (RLNs) and the relationship between regression rate of RLNs and prognosis of patients with locally advanced nasopharyngeal carcinoma (LANPC).

Methods
A total of 122 LANPC patients with RLNs metastasis were included. Metastatic RLNs were delineated both before and after treatment slice by slice on the magnetic resonance images cross-section. The regression rate of RLNs, adverse effects (AE) were evaluated. The median regression rate of RLNs was taken as the cut-off value, and the patients were furtherly divided into high regression rate (HRR) group and low regression rate (LRR) group, then survival times were evaluated.

Results
The median regression rates of RLNs in the ECCRT and CCRT groups were 81% and 50%, respectively (P < 0.001). There was no statistically significant difference in the incidence of grade 3/4 AEs between the two groups, except for oral mucositis (ECCRT 26.23% vs. CCRT 44.26%, P = 0.037). The 3-year overall survival (OS), progression-free survival (PFS), distant metastasis-free survival (DMFS) and locoregional failure-free survival (LRFFS) rates in the HRR and LRR groups were 85.48% and 86.67% (P = 0.983), 80.65% and 68.33% (P = 0.037), 83.87% and 85% (P = 0.704), 93.55% and 81.67% (P = 0.033), respectively.

Conclusions
Patients in the ECCRT group had higher regression rates of RLNs and lower incidence of severe oral mucositis. Furthermore, patients in the HRR group had a better 3-year PFS and LRFFS rate than those in the LRR group.
Chromosome-Y haplogroups in Asturias (Northern Spain) and their association with severe COVID-19
Authors: González-Fernández Mar , Vázquez-Coto Daniel , Albaiceta Guillermo M. , Amado-Rodríguez Laura , Clemente Marta G. , Velázquez-Cuervo Lucinda , García-Lago Claudia , Gómez Juan , Coto Eliecer 
Published: May 2024

AbstractThe main objective of this study was to determine whether the common Y-haplogroups were be associated with the risk of developing severe COVID-19 in Spanish male. We studied 479 patients who required hospitalization due to COVID-19 and 285 population controls from the region of Asturias (northern Spain), They were genotyped for several polymorphisms that define the common European Y-haplogroups. We compared the frequencies between patients and controls aged ≤ 65 and >65 years. There were no different haplogroup frequencies between the two age groups of controls. Haplogroup R1b was less common in patients aged ≤65 years. Haplogroup I was more common in the two patient´s groups compared to controls (p = 0.02). Haplogroup R1b was significantly more frequent among hypertensive patients, without difference between the hypertensive and normotensive controls. This suggested that R1b could increase the risk for severe COVID-19 among male with pre-existing hypertension. In conclusion, we described the Y-haplogroup structure among Asturians. We found an increased risk of severe COVID-19 among haplogroup I carriers, and a significantly higher frequency of R1b among hypertensive patients. These results indicate that Y-chromosome variants could serve as markers to define the risk of developing a severe form of COVID-19.
Reversed-phase thin-layer chromatography and ultra-performance liquid chromatography/mass spectrometry to estimate the drug likeness of phosphodiesterase 10A inhibitors with phthalimide core
Authors: Czopek Anna , Żmudzki Paweł , Dąbrowska Monika , Starek Małgorzata , Łątka Kamil , Bajda Marek , Jaromin Anna , Fryc Monika , Zagórska Agnieszka 
Published: May 2024

AbstractLipophilicity is a physicochemical parameter well known as a decisive factor for predicting the successful development of a drug. Thus, a balance between potency and physicochemical properties during medicinal chemistry optimization is needed. In this study, the lipophilicity of isoindole-1,3(2H)-dione derivatives designed as phosphodiesterase 10A (PDE10A) inhibitors was determined by chromatographic [reversed-phase thin-layer chromatography (RP-TLC) and ultra-performance liquid chromatography/mass spectrometry (UPLC/MS)] and in silico methods. To assess the correlation between the obtained lipophilicity parameters, principal component analysis (PCA) was performed. logP values obtained by chromatographic (logPRP-TLC and logPUPLC/MS) and in silico methods were compared using the PCA method. The results of PCA revealed that logPUPLC/MS and in silico clogP provided by the ChemDraw program were highly correlated. Compounds’ drug likeness was screened, and the pharmacokinetic properties were predicted. All the investigated compounds displayed drug-likeness properties, and they met the criteria of Lipinski’s rule of five, which predicted the oral bioavailability of drug candidates. Analysis of the influence of physicochemical properties on the biological activity showed that the compounds with increased potency on PDE10A had significantly higher topological polar surface area (TPSA) values. The blood‒brain barrier permeability and the hemolytic activity of model compound 18 were examined. The model compound 18 displayed no toxicity effect on erythrocytes in the hemolytic assay and good parallel artificial membrane permeability. The results showed that phthalimide compounds with benzimidazole moiety are a source of compound-targeted inhibition of PDE10A with balanced physicochemical and drug-likeness properties.
The impact of temperature on vascular function in connection with vascular laser treatment
Authors: Doppegieter M. , van Leeuwen T.G. , Aalders M.C.G. , de Vos J. , van Bavel E.T. , Bakker E.N.T.P 
Published: May 2024

Abstract
Pulsed dye lasers are used effectively in the treatment of psoriasis with long remission time and limited side effects. It is, however, not completely understood which biological processes underlie its favorable outcome. Pulsed dye laser treatment at 585-595 nm targets hemoglobin in the blood, inducing local hyperthermia in surrounding blood vessels and adjacent tissues. While the impact of destructive temperatures on blood vessels has been well studied, the effects of lower temperatures on the function of several cell types within the blood vessel wall and its periphery are not known. The aim of our study is to assess the functionality of isolated blood vessels after exposure to moderate hyperthermia (45 to 60°C) by evaluating the function of endothelial cells, smooth muscle cells, and vascular nerves. We measured blood vessel functionality of rat mesenteric arteries (n=19) by measuring vascular contraction and relaxation before and after heating vessels in a wire myograph. To this end, we elicited vascular contraction by addition of either high potassium solution or the thromboxane analogue U46619 to stimulate smooth muscle cells, and electrical field stimulation (EFS) to stimulate nerves. For measurement of endothelium-dependent relaxation, we used methacholine. Each vessel was exposed to one temperature in the range of 45-60°C for 30 seconds and a relative change in functional response after hyperthermia was determined by comparison with the response per stimulus before heating. Non-linear regression was used to fit our dataset to obtain the temperature needed to reduce blood vessel function by 50% (Half maximal effective temperature, ET50). Our findings demonstrate a substantial decrease in relative functional response for all three cell types following exposure to 55°C-60°C. There was no significant difference between the ET50 values of the different cell types, which was between 55.9°C and 56.9°C (P>0.05). Our data show that blood vessel functionality decreases significantly when exposed to temperatures between 55°C-60°C for 30 seconds. The results show functionality of endothelial cells, smooth muscle cells, and vascular nerves is similarly impaired. These results help to understand the biological effects of hyperthermia and may aid in tailoring laser and light strategies for selective photothermolysis that contribute to disease modification of psoriasis after pulsed dye laser treatment.
Physiological effects of interacting native and invasive bivalves under thermal stress
Authors: Hillebrand Veronika , Dobler Andreas H. , Schwalb Astrid N. , Geist Juergen 
Published: May 2024

AbstractAcross many ecosystems in North America and Europe, native freshwater bivalves (Order Unionida) are threatened by fouling and competition for food by the invasive zebra mussel Dreissena polymorpha. In light of climate change, knowledge on the influence of water temperature on these competitive effects is important, yet poorly understood. This study examines the physiological impact of the interaction between D. polymorpha and the native European unionid Anodonta cygnea over a 28 day—period in response to water temperatures of 12, 19, and 25 °C by comparing their glycogen, glucose, lipid and protein concentrations. The laboratory experiment comprised three treatments: (1) fouling of A. cygnea by D. polymorpha, (2) both species present but not fouling; and (3) a control in which A. cygnea and D. polymorpha were placed separately. Increased water temperatures caused physiological stress in D. polymorpha as evident from reduced glycogen, glucose, lipid and protein concentrations. Dreissena polymorpha benefited from fouling of unionids, as individuals that fouled A. cygnea tended to have increased glycogen, glucose, lipid and protein concentrations. Competitive effects of D. polymorpha over the unionid bivalve species, however, were not intensified by elevated temperatures. Glochidia release, lower infestation intensity, and physiological stress of Dreissena at higher temperatures were likely confounding factors. The results of this study suggest that understanding the physiological consequences of species interactions at changing temperatures can be an important tool to assess future climate change impacts on freshwater bivalves and aquatic community structures.
Contribution of marine macrophytes to pCO2 and DOC variations in human-impacted coastal waters
Authors: Watanabe Kenta , Tokoro Tatsuki , Moki Hirotada , Kuwae Tomohiro 
Published: May 2024

AbstractCarbon cycles in coastal waters are highly sensitive to human activities and play important roles in global carbon budgets. CO2 sink–source behavior is regulated by spatiotemporal variations in net biological productivity, but the contribution of macrophyte habitats including macroalgae aquaculture to atmospheric CO2 removal has not been well quantified. We investigated the variations in the carbonate system and dissolved organic carbon (DOC) in human-impacted macrophyte habitats and analyzed the biogeochemical drivers for the variations of these processes. Cultivated macroalgal metabolism (photosynthesis, respiration, calcification, and DOC release) was quantified by in situ field-bag experiments. Cultivated macroalgae took up dissolved inorganic carbon (DIC) (16.2–439 mmol-C m−2 day−1) and released DOC (1.2–146 mmol-C m−2 day−1). We estimated that seagrass beds and macroalgae farming contributed 0.8 and 0.4 mmol-C m−2 day−1 of the in situ total CO2 removal (5.7 and 6.7 mmol-C m−2 day−1, respectively) during their growing period in a semi-enclosed embayment but efficient water exchange (i.e., short residence time) in an open coastal area precluded detection of the contribution of macrophyte habitats to the CO2 removal. Although hydrological processes, biological metabolism, and organic carbon storage processes would contribute to the net CO2 sink–source behavior, our analyses distinguished the contribution of macrophytes from other factors. Our findings imply that macroalgae farming, in addition to restoring and creating macrophyte habitats, has potential for atmospheric CO2 removal.
A novel remote sensing-based approach to determine loss of agricultural soils due to soil sealing — a case study in Germany
Authors: Säurich Annelie , Möller Markus , Gerighausen Heike 
Published: May 2024

AbstractSoils provide habitat, regulation and utilization functions. Therefore, Germany aims to reduce soil sealing to 30 ha day$$^{-1}$$

-
1

by 2030 and to eliminate it by 2050. About 55 ha day$$^{-1}$$

-
1

of soil are damaged (average 2018–2021), but detailed information on its soil quality is lacking. This study proposes a new approach using geo-information and remote sensing data to assess agricultural soil loss in Lower Saxony and Brandenburg. Soil quality is assessed based on erosion resistance, runoff regulation, filter functions, yield potential and the Müncheberg Soil Quality Rating from 2006 to 2015. Data from the German Soil Map at a scale of 1:200,000 (BÜK 200), climate, topography, CORINE Land Cover (CLC) and Imperviousness Layer (IMCC), both provided by the Copernicus Land Monitoring Service (CLMS), are used to generate information on soil functions, potentials and agricultural soil loss due to sealing. For the first time, soil losses under arable land are assessed spatially, quantitatively and qualitatively. An estimate of the qualitative loss of agricultural soil in Germany between 2006 and 2015 is obtained by intersecting the soil evaluation results with the quantitative soil loss according to IMCC. Between 2006 and 2015, about 73,300 ha of land were sealed in Germany, affecting about 37,000 ha of agricultural soils. This corresponds to a sealing rate of 11 ha per day for Germany. In Lower Saxony and Brandenburg, agricultural soils were sealed at a rate of 1.9 ha day$$^{-1}$$

-
1

and 0.8 ha day$$^{-1}$$

-
1

respectively, removing these soils from primary land use. In Lower Saxony, 75% of soils with moderate or better biotic yield potential have been removed from primary land use, while in Brandenburg this figure is as high as 88%. Implementing this approach can help decision-makers reassess sealed land and support Germany’s sustainable development strategy.
The Simons Observatory: Production-Level Fabrication of the Mid- and Ultra-High-Frequency Wafers
Authors: Duff Shannon M. , Austermann Jason , Beall James A. , Daniel David P. , Hubmayr Johannes , Jaehnig Greg C. , Johnson Bradley R. , Jones Dante , Link Michael J. , Lucas Tammy J. , Sonka Rita F. , Staggs Suzanne T. , Ullom Joel , Wang Yuhan 
Published: May 2024

AbstractThe Simons Observatory (SO) is a cosmic microwave background instrumentation suite in the Atacama Desert of Chile. More than 65,000 polarization-sensitive transition-edge sensor (TES) bolometers will be fielded in the frequency range spanning 27 to 280 GHz, with three separate dichroic designs. The mid-frequency 90/150 GHz and ultra-high-frequency 220/280 GHz detector arrays, fabricated at NIST, account for 39 of 49 total detector modules and implement the feedhorn-fed orthomode transducer-coupled TES bolometer architecture. A robust production-level fabrication framework for these detector arrays and the monolithic DC/RF routing wafers has been developed, which includes single device prototyping, process monitoring techniques, in-process metrology, and cryogenic measurements of critical film properties. Application of this framework has resulted in timely delivery of nearly 100 total superconducting focal plane components to SO with $$88\%$$

88
%

of detector wafers meeting nominal criteria for integration into a detector module: a channel yield $$>95\%$$

>
95
%

and $$T_{\textrm{c}}$$

T
c

in the targeted range.
The Relationship Between Strategic Human Resource Management Practices and the Employment of Vulnerable Workers: A Two-Wave Study Among Employers
Authors: Kersten Amber , van Woerkom Marianne , Geuskens Goedele A. , Blonk Roland W. B. 
Published: May 2024

Abstract
Purpose
To improve the inclusion of vulnerable workers in the labor market, employer behavior is key. However, little is known about the effectiveness of strategic Human Resource Management (HRM) practices that employers use to employ vulnerable workers. Therefore, this exploratory study investigates the association between strategic HRM practices (based on social legitimacy, economic rationality and employee well-being) and the actual and intended employment of vulnerable workers in the future.

Methods
In total, 438 organizations included in the Netherlands Employers Work Survey participated in a two-wave study with a nine-month follow-up period. Logistic regression models were used to estimate the relationship between strategic HRM practices (T0) with the employment of vulnerable workers (T1) and intentions to hire vulnerable workers (T1), while controlling for organizational size, sector, and employment of vulnerable workers at baseline.

Results
Employers who applied strategic HRM practices based on social legitimacy (e.g., inclusive mission statement or inclusive recruitment) or economic rationality (e.g., making use of reimbursements, trial placements, or subsidies) at T0 were more likely to employ vulnerable workers and to intend to hire additional vulnerable workers at T1. No significant results were found for practices related to employee well-being.

Conclusion
Since different types of strategic HRM practices contribute to the inclusion of vulnerable workers, employers can build on their strategic priorities and strengths to create inclusive HRM approaches. Future research is needed to study whether these strategic HRM domains also relate to sustainable employment of vulnerable workers.
Topological twists of massive SQCD, Part I
Authors: Aspman Johannes , Furrer Elias , Manschot Jan 
Published: May 2024

AbstractWe consider topological twists of four-dimensional $$\mathcal {N}=2$$

N
=
2

supersymmetric QCD with gauge group SU(2) and $$N_f\le 3$$

N
f

≤
3

fundamental hypermultiplets. The twists are labelled by a choice of background fluxes for the flavour group, which provides an infinite family of topological partition functions. In this Part I, we demonstrate that in the presence of such fluxes the theories can be formulated for arbitrary gauge bundles on a compact four-manifold. Moreover, we consider arbitrary masses for the hypermultiplets, which introduce new intricacies for the evaluation of the low-energy path integral on the Coulomb branch. We develop techniques for the evaluation of these path integrals. In the forthcoming Part II, we will deal with the explicit evaluation.
Growth of Nordic container forest tree seedlings in some peatless and peat-reduced growing media
Authors: Heiskanen Juha , Ruhanen Hanna , Himanen Katri , Kivimäenpää Minna , Silvan Niko 
Published: May 2024

AbstractCarbon emissions and environmental concerns have led to the aspiration to reduce peat extraction and its use as a growing medium (GM). In Nordic Forest tree seedling production, Sphagnum peat has been almost exclusively used as a GM in seedling containers due to its good properties and availability. This study examined the feasibility of several peat-reduced and peat-free GM in container tree seedling production of the key tree species in Nordic forestry (Scots pine (Pinus sylvestris L.), Norway spruce (Picea abies L. Karst.) and silver birch (Betula pendula Roth) as well as Russian larch (Larix archangelica P. Lawson & C. Lawson ex Trautv.) and common alder (Alnus glutinosa (L.) Gaertn.). Pure or mixed GM, consisting of low-humified Sphagnum peat (pure as a control), harvested Sphagnum moss, wood fiber, cow manure digestate from a biogas plant, and a common reed compost were tested. Seedlings were grown in controlled conditions in greenhouse experiments and also in larger-scale commercial tree nurseries. Peat-reduced media containing peat of at least 50 vol% provided growth that is similar to pure Sphagnum peat for the tested species and container types. All the studied alternative media can yield marketable seedlings, although commonly of reduced size and requiring special adjustments in growing management. The studied media have a potential to reduce or replace peat in seedling production, but adjustments of their physical and chemical properties, as well as of seedling fertigation and management procedures, are required. The economic feasibility and environmental sustainability of these GM, as well as the outplanting success of seedlings grown in these media, remain to be studied.
Does Financial Inclusion Matter to Population Health? Insight From a Global Dataset
Authors: Acheampong Alex O. , Tetteh Godsway Korku 
Published: May 2024

AbstractCurrently, extensive empirical studies exist on the impact of financial inclusion on socio-economic outcomes such as economic growth, inequality, and poverty. However, research on financial inclusion and population health is limited and still at the infant stage. Therefore, this article adds to knowledge by inquiring into the health implications of financial inclusion using a global panel dataset for 121 countries between 2004 and 2020. After accounting for endogeneity with heteroskedasticity-based instrumental variable regression and cross-sectional dependency with the Driscol–Kraay estimator, we documented that financial inclusion improves population health outcomes. We found that the effect of financial inclusion on population health outcomes also depends on the level of information and communication technology penetration and existing socio-economic conditions in a country. Following this, we showed that financial inclusion improves population health outcomes in countries with lower income inequality and higher GDP per capita, mobile and internet penetration. We further found heterogeneity in the results across geographical regions and income groups. Following these findings, we suggest that financial inclusion policies could enhance population health.