bluuebunny/crossref_metadata_2025_split huggingface dataset:
Calix[6]arene‐Picolinamide Extractants for Radioactive Waste Treatment: Effect of Additional Carboxy Binding Sites in the Pyridine 6‐Positions on Complexation, Extraction Efficiency and An/Ln Separation (Eur. J. Org. Chem. 14/2010)
Authors: Macerata Elena , Sansone Francesco , Baldini Laura , Ugozzoli Franco , Brisach Frederic , Haddaoui Jaouad , Hubscher‐Bruder Véronique , Arnaud‐Neu Françoise , Mariani Mario , Ungaro Rocco , Casnati Alessandro 
Published: April 2010

AbstractThe cover picture shows the complexation of actinide metal ions by picolinamide‐based calix[6]arenes. These ligands possess remarkable efficiency in the extraction of trivalent metal ions even at very high HNO3 concentration and fair selectivity for actinide ions over lanthanide ions, which is useful in the recycling of the spent nuclear fuel. The Partitioning and Transmutation (P&T) strategy would allow the long‐lived minor actinides (Np, Am, Cm) in fast nuclear reactors to be separated and transmutated and would also allow the radiotoxicity in the ultimate waste to be minimized, making nuclear power plants more efficient and sustainable. Details are discussed in the article by M. Mariani, A. Casnati et al. on p. 2675 ff.
Dual CD system in capillary electrophoresis for direct separation of the four stereoisomers of agonist and antagonist melatoninergic ligands
Authors: Lipka Emmanuelle , Danel Cécile , Yous Saïd , Bonte Jean‐Paul , Vaccher Claude 
Published: April 2010

AbstractIn this study, baseline separation of the stereoisomers of six tetrahydronaphthalenic derivatives (agonists and antagonists for the melatonin (N‐acetyl‐5‐methoxytryptamin) binding sites) was successfully achieved using CE and CDs as chiral selectors. The method for the simultaneous chiral separation of the four stereoisomers uses a capillary dynamically coated with polyethylene oxide and a dual CD system. Optimisation was performed first upon the constituents of the CD system, by varying neutral and anionic CD type, size and concentration, at first in mono‐CD systems and subsequently in dual neutral/anionic CD systems. Once these characteristics of the dual CD system were established, operational parameters such as voltage and temperature were then optimised. Under the optimal conditions (i.e. 1.5% w/v of highly S‐β‐CD and 10 mM of γ‐CD in 25 mM phosphate buffer (pH 2.5) as the BGE, separation voltage 20 kV and a temperature of 25°C), complete resolution of the six molecules was accomplished. Preliminary results for repeatability and the migration order of the optimised method are described.
Enantioseparation of amino acids and α‐hydroxy acids on ligand‐exchange continuous beds by capillary electrochromatography
Authors: Puchalska Patrycja , Pittler Elfriede , Trojanowicz Marek , Gübitz Gerald , Schmid Martin G. 
Published: April 2010

AbstractA new chiral stationary phase based on continuous bed (CB) technology using L‐prolinamide as a chiral selector was prepared. Its ability for enantioseparation of amino acids and α‐hydroxy acids by ligand‐exchange CEC was compared with that of a CB containing L‐4‐hydroxyproline as chiral selector. Preparation was done by a one‐step in situ copolyermerization procedure using methacrylamide as monomer, vinylsulfonic acid as charge providing agent and piperazine diacrylamide as crosslinker. L‐Prolinamide showed marked enantioselective properties for the separation of amino acids and α‐hydroxy acids. Furthermore, we show the possibility of simultaneous enantioseparation of amino acids and α‐hydroxy acids using the CB with L‐4‐hydroxyproline as chiral selector.
Imaging genetics and development: Challenges and promises
Authors: Casey B.J. , Soliman Fatima , Bath Kevin G. , Glatt Charles E. 
Published: April 2010

AbstractExcitement with the publication of the human genome has served as catalyst for scientists to uncover the functions of specific genes. The main avenues for understanding gene function have been in behavioral genetics on one end and on the other end, molecular mouse models. Attempts to bridge these approaches have used brain imaging to conveniently link anatomical abnormalities seen in knockout/transgenic mouse models and abnormal patterns of brain activity seen in humans. Although a convenient approach, this article provides examples of challenges for imaging genetics, its application to developmental questions, and promises for future directions. Attempts to link genes, brain, and behavior using behavioral genetics, imaging genetics, and mouse models of behavior are described. Each of these approaches alone, provide limited information on gene function in complex human behavior, but together, they are forming bridges between animal models and human psychiatric disorders. Hum Brain Mapp, 2010. © 2010 Wiley‐Liss, Inc.
Enlarged tracheoesophageal puncture after total laryngectomy: A systematic review and meta‐analysis
Authors: Hutcheson Katherine A. , Lewin Jan S. , Sturgis Erich M. , Kapadia Asha , Risser Jan 
Published: April 2010

AbstractBackgroundEnlargement of the tracheoesophageal puncture (TEP) is a challenging complication after laryngectomy with TEP. We sought to estimate the rate of enlarged puncture, associated pneumonia rates, potential risk factors, and conservative treatments excluding complete surgical TEP closure.MethodsA systematic review was conducted (1978–2008). A summary risk estimate was calculated using a random‐effects meta‐analysis model.ResultsTwenty‐seven peer‐reviewed manuscripts were included. The rate of enlarged puncture and/or leakage around the prosthesis was reported in 23 articles (range, 1% to 29%; summary risk estimate, 7.2%; 95% confidence interval [CI], 4.8% to 9.6%). Temporary removal of the prosthesis and TEP‐site injections were the most commonly reported conservative treatments. Prosthetic diameter (p = .076) and timing of TEP (p = .297) were analyzed as risk factors; however, radiotherapy variables were inconsistently reported.ConclusionThe overall risk of enlarged puncture seems relatively low, but it remains a rehabilitative challenge. Future research should clearly establish risk factors for enlarged puncture and optimal conservative management. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Technical refinement of ultrasound‐guided transoral resection of parapharyngeal/retropharyngeal thyroid carcinoma metastases
Authors: Andrews Genevieve A. , Kwon Michael , Clayman Gary , Edeiken Beth , Kupferman Michael E. 
Published: April 2010

AbstractBackgroundThe transoral approach to the parapharyngeal and retropharyngeal space (PPS/RPS) for the management of well‐differentiated thyroid carcinoma (WDTC) has been previously described in other articles. However, limited exposure with this approach can be a challenge.MethodsThis is a retrospective review of 6 patients who underwent ultrasound‐guided transoral excision of PPS/RPS WDTC metastases from October 2003 to March 2009 in a cancer center setting. Ultrasound‐guided methylene blue dye injection of the node was used in 3 patients to facilitate intraoperative identification. The technique, safety, and feasibility of the procedure are described in this study.ResultsSuccessful resection of the metastases was accomplished in all cases without intraoperative complication. The 1 definite recurrence was further treated with transmandibular excision.ConclusionTransoral excision of PPS/RPS WDTC metastases with ultrasound‐guided methylene blue dye injection into the metastatic node is safe, feasible, and may further improve intraoperative identification of metastases in poorly accessible locations in the head and neck. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Filamin a mediates HGF/c‐MET signaling in tumor cell migration
Authors: Zhou Alex‐Xianghua , Toylu Aslı , Nallapalli Rajesh K. , Nilsson Gisela , Atabey Neşe , Heldin Carl‐Henrik , Borén Jan , Bergo Martin O. , Akyürek Levent M. 
Published: April 2010

AbstractDeregulated hepatocyte growth factor (HGF)/c‐MET axis has been correlated with poor clinical outcome and drug resistance in many human cancers. Identification of novel regulatory mechanisms influencing HGF/c‐MET signaling may therefore be necessary to develop more effective cancer therapies. In our study, we show that multiple human cancer tissues and cells express filamin A (FLNA), a large cytoskeletal actin‐binding protein, and expression of c‐MET is significantly reduced in human tumor cells deficient for FLNA. The FLNA‐deficient tumor cells exhibited poor migrative and invasive ability in response to HGF. On the other hand, the anchorage‐dependent and independent tumor cell proliferation was not altered by HGF. The FLNA‐deficiency specifically attenuated the activation of the c‐MET downstream signaling molecule AKT in response to HGF stimulation. Furthermore, FLNA enhanced c‐MET promoter activity by its binding to SMAD2. The impact of FLNA deficiency on c‐MET expression and HGF‐mediated cell migration in human tumor cells was confirmed in primary mouse embryonic fibroblasts deficient for Flna. These data suggest that FLNA is one of the important regulators of c‐MET signaling and HGF‐induced tumor cell migration.
Imatinib response in two GIST patients carrying two hitherto functionally uncharacterized PDGFRA mutations: An imaging, biochemical and molecular modeling study
Authors: Dileo Palma , Pricl Sabrina , Tamborini Elena , Negri Tiziana , Stacchiotti Silvia , Gronchi Alessandro , Posocco Paola , Laurini Erik , Coco Paola , Fumagalli Elena , Casali Paolo G. , Pilotti Silvana 
Published: April 2010

AbstractBeside the well known “in vivo” and “in vitro” Imatinib resistant D842V mutation in PDGFRA receptor, very few are the information concerning the “in vivo” Imatinib activity with respect to the other PDGFRA mutations for which only “in vitro” data are available. Two patients carrying PDGFRA mutations in exons 18 (involving residues DIMH842‐845) and 12 (V561D), respectively, were treated with Imatinib at a dose of 400 mg/day. According to Response Evaluation Criteria in Solid Tumors criteria, after a median treatment of 7 months both patients showed clinical partial response, and underwent surgery of the minimal residual disease. Tumor response was confirmed pathologically. In both patients, analyses of PDGFRA performed on pre‐ and/or post‐treatment material were compared to affinity data of the mutated receptor towards the inhibitor. Molecular modeling evidence was found to be consistent with sensitivity of mutated PDGFRA receptors to Imatinib. Thus, the “in vivo” evidence that these two mutations of PDGFRA are sensitive to Imatinib was confirmed by a multidimensional approach comprising “in silico” experiments that, in association to molecular and biochemical analyses, constitutes a powerful tool to predict Imatinib sensitivity, clinically beneficial in the treatment of these tumors with molecularly targeted therapies.
Protein causes a glycemic response
Authors: Whelan William J. , Ghanchi Hammad , Ricciardi Michael 
Published: April 2010

AbstractThe glycemic index is used to compare the extent to which the blood glucose level increases following the consumption of foods containing digestible carbohydrate and is considered to be zero, or not measurable, if the food, such as protein, is carbohydrate‐free. We have found that after overnight fasting, the consumption of several varieties of meat caused significant increases in blood glucose levels. We consider these possibly to be because of gluconeogenesis from the digested protein. It is a curious feature that in two instances the response was inversely related to the amount of meat consumed, over the range from 26 to 78 g of protein. © 2010 IUBMB IUBMB Life, 62(6): 481–483, 2010
Type‐IV pili spectroscopic markers: Applications in the quantification of piliation levels inMoraxella boviscells by a FT‐IR ANN‐based model
Authors: Bosch Alejandra , Prieto Claudia , Serra Diego Omar , Martina Pablo , Stämmler Maren , Naumann Dieter , Schmitt Jürgen , Yantorno Osvaldo 
Published: April 2010

AbstractType‐IV pili are cell surface organelles found in a wide variety of Gram‐negative bacteria. They have traditionally been detected by electron microscopy and ELISA techniques. However, these methodologies are not appropriate for the rapid discrimination and quantification of piliated and nonpiliated cells in industrial or field conditions. Here, the analysis of FT‐IR spectra of piliated, nonpiliated and shearedMoraxella boviscells, together with purified pili suspensions spectra, allowed the identification of 3 IR regions associated to spectroscopic markers of Type‐IV pili: 1750–1600, 1450–1350 and 1280–950 cm–1. Such IR‐specific markers were found for piliated cells grown in different culture systems (liquid or solid media), independently of the strain or pili serotype. They were also sensitive to pili expression levels. Therefore, on the bases of these specific spectral features, an FT‐IR ANN‐based model was developed to classify piliation levels in 5 distinct groups. An overall classification rate of almost 90% demonstrates the strong potential of the ANN system developed to monitorM. boviscultures in vaccine production. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Phase‐contrast hard X‐ray microscopy using synchrotron radiation for the diagnosis of onychomycosis
Authors: Lee Onseok , Ha Seunghan , Lee Gunwoo , Kim Jaeyoung , Huang Jungyun , Jin Kyeongsik , Oh Chilhwan 
Published: April 2010

AbstractOnychomycosis, or fungal infection of the nail, is a disease seen frequently in clinical settings. However, the rates of positive identification using potassium hydroxide preparations or fungal cultures are relatively low. Precise diagnosis is possible via histopathologic examination to monitor the existence of fungus and performance of a fungal culture for confirmation. Phase‐contrast hard X‐ray microscopy using synchrotron radiation provides 70‐nm spatial resolution and enables imaging of minute internal cellular structures. This study confirms the feasibility of diagnosing onychomycosis using a phase‐contrast hard X‐ray microscope developed at 1B2 beam line using a Pohang light source. Microsc. Res. Tech. 73:1110–1114, 2010. © 2010 Wiley‐Liss, Inc.
Evaluation of the Environmental Protection Agency/National Weather Service Ultraviolet Index Forecast against independent UV measurements: Phoenix Arizona (2000–2006)
Authors: Svoma Bohumil M. , DeBiasse Kimberly , Nolte Jessica , Busby Bradley , Beeson Carolyn , Cerveny Randall 
Published: April 2010

AbstractThe effectiveness of the National Weather Service (NWS) Ultraviolet Index (UVI) forecast is evaluated for one of the largest United States metropolitan areas that annually experiences exceedingly large dosages of ultraviolet (UV) radiation, Phoenix Arizona (AZ). Data were collected from Arizona Desert Testing LLC sited at Wittmann AZ, the Volkswagen Arizona Proving Grounds sited at Maricopa AZ and Atlas Material Testing Technology LLC, DSET Laboratories sited at New River AZ. In general, over 50% of the variance (r = 0.709) in UV radiation is shared between the datasets from the three independent facilities although the geographic distances between sites are relatively large. Overall correlations between the NWS UVI forecasts and the data from the three independent testing facilities are surprisingly weak when the annual cycle is removed from all datasets with less than 21% of the variance in UV data accounted for by the NWS UVI forecast. The lowest correspondence occurred during the summer months with June displaying the lowest correlations, often insignificant at the 0.05 level. To account for these lower correlations, we considered daily sky cover and air quality observations of particles with a diameter less than 10 µm (PM10). Our analyses suggest that large amounts of PM10 present during the late spring and early summer are a likely cause for low correspondence between the NWS UVI and UV data in June. Given the unexpectedly low overall correlations, optical depth may need to be better incorporated in the NWS UVI forecast process through pollution forecasts in order to increase the correspondence between the NWS UVI and actual UV irradiance. Copyright © 2010 Royal Meteorological Society
Early‐onset metaphyseal chondrodysplasia type Schmid associated with a COL10A1 frame‐shift mutation and impaired trimerization of wild‐type α1(X) protein chains
Authors: Mäkitie Outi , Susic Miki , Cole William G. 
Published: April 2010

AbstractBoth dominant‐negative and haploinsufficiency effects have been proposed in the pathogenesis of metaphyseal chondrodysplasia type Schmid (MCDS) due to nonsense and frame‐shift mutations of COL10A1. This study examines these alternative effects. A proband with typical early‐onset MCDS was ascertained and COL10A1 sequencing undertaken. The assembly of trimeric collagen X molecules was studied using in vitro coupled transcription and translation of wild‐type and mutant α1(X) cDNAs. The proband was heterozygous for a unique COL10A1 mutation, c.1735_1739del5ins22. Mutant protein chains, with the corresponding p.G579fsX611 change, failed to spontaneously trimerize. When wild‐type α1(X) chains were translated alone, 57 ± 7% of the chains assembled into stable collagen X trimers. Trimerization of wild‐type chains was significantly reduced to 33 ± 6% when translated in 1:1 mixtures with p.G579fsX611 α1(X) chains. The protein assembly assay showed that the mutant chains exerted a dominant‐negative effect on collagen X assembly. Previous studies indicate that nonsense‐mediated decay, activation of endoplasmic reticulum, and unfolded protein responses as well as altered chondrocyte differentiation are the major determinants of phenotypic severity and age of presentation. We speculate that complete loss of mutant transcripts yields COL10A1 haploinsufficiency and late clinical presentation while incomplete loss of mutant transcripts yields dominant‐negative effects with early clinical presentation. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1497–1501, 2010
The effects of sintering and shot peening processes on the fatigue strength
Authors: Iynen O. , Eksi A. K. , Bircan D. A. , Dilek M. 
Published: April 2010

AbstractAlumix 431 metal powders have been used in this study since they are widely used in the industrial applications. First, Alumix 431 powders were compacted at 400 MPa pressure under room temperature (RT). Then, pressed specimens were sintered separately at 600 and 620°C for 1h N2. Densities were measured for the different temperatures in order to examine the effects of the sintering on density. Improvement of green density was observed to have enhanced approximately 86 and 90% according to theoretical density (2, 786 g/cm3) as a result of the sintering condition at 600 and 620°C, respectively. After sintering a group of specimen was shot peened with the intensities in 12 and 16A. To investigate mechanical properties of as sintered (unpeened) and shot peened samples, tensile and fatigue tests were performed. It was determined that shot peening improved the fatigue strength about 10 and 15% in 12 and 16A intensities, respectively. In addition, fractured specimens after tensile tests were used for Micro‐Hardness measurements, and for metallographic analysis by, optical microscopy (OM) and scanning electron microscopy (SEM) tests further specimens were examined.Für diese Arbeit wurden die Metallpulver Alumix 431 ausgewählt, die in der industriellen Praxis häufig verwendet werden. Zuerst wurde das Metallpulver Alumix 431 bei einem Pressdruck 400 MPa unter Raumtemperatur kompaktiert. Danach wurden die gepressten Proben bei 600 und 620°C Temperatur jeweils 60 Minuten in N2‐Atmospäre gesintert. Um die Auswirkung des Sinterns auf die Dichte zu beobachten, wurden bei unterschiedlichen Temperaturen hergestellte Proben untersucht. Nach der Kompaktierung wurden unter den Temperaturen 600 und 620°C 86% bzw. 90% der theoretischen Dichte erreicht. Dies zeigt, dass die gepresste Dichte durch das unterschiedliche Sinterlegierungsverhalten zunimmt. Nach der Sinterung wurde mit einer Intensität von 12A und 16A ein Teil der Proben kugelgestrahlt. Um die Eigenschaften der gestrahlten und nicht gestrahlten Proben zu untersuchen, wurden Ermüdungsfestigkeitsversuche durchgeführt. Das Kugelstrahlen erhöht die Ermüdungsfestigkeit etwa um 10 bis 15%. Außerdem wurden die nach dem Zugversuch erhaltenen Bruchstücke für Mikrohärtenmessungen verwendet. Die metallographischen Untersuchungen der Proben wurde im Licht‐Mikroskop (OM) und Raster‐Elektronenmikroskop (SEM) untersucht.
The equivalent stress on the critical plane determined by the maximum covariance of normal and shear stresses
Authors: Walat Karolina , Lagoda Tadeusz 
Published: April 2010

AbstractThe paper presents a new form of the stress criterion of multiaxial random fatigue. The criterion has been defined as a sum of normal and shear stresses with weight coefficients on the critical plane defined as the plane where the maximum covariance between shear and normal stresses occurs. Two forms of the criterion have been proposed.
Evaluation of vehicle substances on vitamin D bioavailability: A systematic review
Authors: Grossmann Ruth E. , Tangpricha Vin 
Published: April 2010

AbstractVitamin D insufficiency is a common medical condition. Vitamin supplements can be ingested to improve vitamin D status. It is not known if the vehicle substance that is combined with the vitamin D tablet influences the bioavailability of vitamin D. The purpose of this review is to examine the impact of different vehicles on vitamin D bioavailability. A comprehensive literature search identified studies that directly compared the absorption of vitamin D from two or more vehicles. The change in mean serum 25(OH)D per average daily dose of vitamin D supplemented was calculated and compared among the studies. We identified four clinical studies that compared two different vehicles of vitamin D. Vitamin D in an oil vehicle produced a greater 25(OH)D response than vitamin D in a powder or an ethanol vehicle in healthy subjects. There are limited studies that have compared the influence of the vehicle substance on vitamin D bioavailability. Future studies should examine bioavailability among different vehicle substances such as oil, lactose powder, and ethanol and examine if there are any differences in bioavailability among different patient populations including those with fat malabsorption.