bluuebunny/crossref_metadata_2025_split huggingface dataset:
Attitudes and Beliefs of Secondary Teachers about Internet Use in their Classrooms
Authors: Ramírez-Orellana Elena , Cañedo-Hernández Isabel , Clemente-Linuesa María 
Published: April 2012

The work below examines the attitudes and beliefs that secondary teachers have about the use of Internet resources in their practices. It is presented results obtained from a questionnaire (n=1,721) with different dimensions, obtaining data about teachers’ attitudes and reasons with respect to the use of Internet resources, respect to teachers’ tasks employed for and respect to training teachers received about Internet resources. The study use descriptive and correlation data of answers percentages to questionnaire items. Results show that attitudes are very important to explain the use of these resources in classroom practices. Also, the role that teachers’ perception about their digital competencies plays over the possibility to use Internet resources in their classroom practices. These results show differences between teacher in function of age and genre. Finally, teachers’ training about Internet has a positive effect on self perception about digital competencies. The results point to the need of research on what beliefs could explain why teachers decide to use or not Internet resources, how they use them and which factors are included.
En este trabajo se estudian las actitudes y creencias que los profesores de secundaria tienen sobre la utilización de los recursos de Internet en sus prácticas. Se presentan los resultados obtenidos aplicando un cuestionario (n=1.721) que abarca diversas dimensiones, obteniéndose datos respecto de las actitudes de los profesores en relación al uso o no de recursos de Internet, a las tareas docentes para las que se emplean y con la formación recibida al respecto. Se ofrecen datos de los porcentajes de respuestas a los ítems del cuestionario e índices correlacionales. Entre los resultados destaca la relación entre las actitudes de los profesores y el que introduzcan estos recursos en sus prácticas, así como el papel que juega la edad y el sexo de los profesores en estas actitudes. También se pone de relieve la relación entre la creencia de los profesores en su competencia digital y la probabilidad de que utilicen los recursos de la Red en sus prácticas. Por último, lo resultados destacan la relación que aparece entre la formación recibida sobre Internet y las diferencias en la percepción sobre competencia digital de los docentes. Los resultados subrayan la necesidad de estudiar en profundidad cuáles son las creencias que explican de forma específica la adopción o no de estos recursos digitales, cómo se conforman y qué elementos las definen.
Media Literacy and Consumption of Media and Advertising in University Students of Pedagogy in Chile
Authors: del-Valle-Rojas Carlos , Denegri-Coria Marianela , Chávez-Herting David 
Published: April 2012

Considering the results of studies on the mediating role of the media, specially the television, and the advertising in different groups etarios, added to the need to discuss the processes of audio-visual literacy or media literacy; the principal aim of this work was analyzed the pattern of consumption of media in students of pedagogy in Language and Communication, Mathematics, and History and Geography of Chilean universities and to determine if differential profiles exist in the use and consumption of media that you relate to his future role in the audio-visual literacy of his students. For it, it was considered to be a sample of meaningful type remained constituted by 881 students of pedagogy of both sexes who were dealing studies in seven Chilean universities of the south and central zone of Chile. For the compilation of information there was in use the «questionnaire of habits of consumption of media». In general, the distribution of the preferences, so much to level of kind as for career, does not manage to represent mediatically the idiosyncrasy and reflects foreign realities that do not allow to strengthen civil processes in the consumers of the media. In synthesis, the major problematics is that the educational futures do not seem to rely on tools that they should allow them to face the labor of audio-visual literacy, provided that they themselves present difficulties to differ between the information and the persuasion in the advertising messages.Considerando los resultados de estudios sobre el rol mediador de los medios, especialmente la televisión y la publicidad en distintos grupos etarios, sumados a la necesidad de discutir los procesos de alfabetización audiovisual o alfabetización mediática; el principal objetivo de este trabajo fue analizar los patrones de consumo de medios en estudiantes de Pedagogía en Lenguaje y Comunicación, Matemáticas, e Historia y Geografía de universidades chilenas y determinar si existen perfiles diferenciales en el uso y consumo de medios que se relacionen con su futuro rol en la alfabetización audiovisual de sus estudiantes. Para ello, se consideró una muestra de tipo intencionado que quedó constituida por 881 estudiantes de pedagogía de ambos sexos que cursaban estudios en siete universidades chilenas de la zona sur y central de Chile. Para la recolección de datos se utilizó el «cuestionario de hábitos de consumo de medios». En general, la distribución de las preferencias, tanto a nivel de género como por carrera, no logra representar mediáticamente la idiosincrasia y refleja realidades ajenas que no permiten fortalecer procesos ciudadanos en los consumidores de los medios. En síntesis, la problemática mayor es que los futuros docentes no parecen contar con herramientas que les permitan enfrentar la labor de alfabetización audiovisual, dado que ellos mismos presentan dificultades para diferenciar entre la información y la persuasión en los mensajes publicitarios.
The Effect of Peripherally Inserted Central Catheter (PICC) Valve Technology on Catheter Occlusion Rates - The ‘ELeCTRiC’ Study
Authors: Johnston Andrew J. , Streater Carmel T. , Noorani Remy , Crofts Joanne L. , Del Mundo Aldwin B. , Parker Richard A. 
Published: April 2012

Purpose Peripherally Inserted Central Catheters (PICCs) are increasingly being used to provide short to medium-term central venous access. The current study was designed to test the hypothesis that PICC valve technology does not influence PICC occlusion rates. Methods Intensive care unit (ICU) patients who required a PICC were randomized to one of three types of dual lumen PICC (open ended non-valved, Groshong valve, PASV valve). PICC occlusions were recorded and managed with a protocol that used urokinase. Results A total of 102 patients were recruited to the study. The overall risk of occlusion per catheter was 35% (95% CI 26% to 44%). The overall rate of occlusion was 76 occlusions per 1000 catheter days (95% CI 61 to 95). Presence or type of valve did not significantly influence this rate (open-ended non-valved PICC 38% of catheters, 79 occlusions per 1000 catheter days; Groshong 38% of catheters, 60 occlusions per 1000 catheter days; PASV 27% of catheters, 99 occlusions per 1000 catheter days). The dose of urokinase required to treat PICC occlusions did not significantly differ between PICC types. Conclusions Valved PICCs do not appear to influence PICC occlusion rates.
L’agir politique au regard des technologies de l’information et de la communication
Authors: Goldenberg Anne , Proulx Serge 
Published: April 2012

La gauche québécoise peut-elle inventer de nouveaux espaces citoyens à partir des configurations sociopolitiques ouvertes par les technologies de l’information et de la communication (TIC) ? Nous avons repéré trois formes de militantisme à l’oeuvre dans ce nouveau contexte. Un premier militantisme oriente ses actions vers un accès élargi aux moyens techniques. Issus du milieu communautaire, des militants, hommes et femmes, s’assurent que le plus grand nombre puisse utiliser les technologies, les comprendre, les maîtriser, tout en se maintenant à distance des logiques commerciales sous-jacentes au développement technologique. Or, dans un contexte fortement marqué par l’idéologie néolibérale, les milieux communautaires sont aujourd’hui contraints de réorienter leurs modes d’intervention ou de financement, ce qui complexifie leurs actions pour l’accessibilité. Un second militantisme fait plus directement usage des médias. Ainsi, des groupes activistes ont pris l’habitude de publiciser eux-mêmes leurs actions. Par ce moyen, ils proposent une signification globale à leurs actions locales. Ils ont appris à reconsidérer leurs relations aux technologies et à la communication. Face à l’invasion des pratiques publicitaires, certains de ces activistes s’organisent aussi pour subvertir les messages au sein même des espaces de propagande et de marketing. Décriant la privatisation croissante des médias québécois, d’autres initiatives ont émergé pour fournir une option de qualité, portée par des amateurs, mais aussi par des journalistes libérés de leurs contraintes habituelles. Un troisième militantisme s’attaque plus directement à la conception même et à la propriété des dispositifs communicationnels. Ces groupes « activistes de la technique » revendiquent un droit de regard et d’intervention sur la conception et le fonctionnement même des dispositifs sociotechniques qui nous entourent. Ils militent à la fois pour la production de dispositifs au code librement accessible, pour la mise à disposition de biens communs informationnels et pour un libre accès aux connaissances. Comment ces trois formes de militantisme sont-elles reliées ? Dans quelle mesure ces nouveaux militantismes témoignent-ils d’un mouvement global de reconfiguration de l’agir politique ? Ces formes de militance orientées vers la technique peuvent-elles avoir une portée politique à plus long terme ?
Le corps accidenté chez Hélène Monette et Élise Turcotte
Authors: Parent Marie 
Published: April 2012

Cet article propose d’interroger une fois de plus le concept d’américanité, cette fois-ci à partir de deux textes d’écrivaines québécoises contemporaines, Unless d’Hélène Monette et Pourquoi faire une maison avec ses morts d’Élise Turcotte. À travers la figure du corps « accidenté » sera mise en évidence la relation de réciprocité établie dans ces textes entre corps et territoire : la représentation du corps blessé, souffrant est indissociable de l’expérience d’une Amérique torturée, hantée par le sentiment de sa fin imminente. Corps et continent, pris l’un pour l’autre, trahissent ici le même malaise, et fondent d’une manière particulière la difficulté à habiter le monde.
RASL‐seq for Massively Parallel and Quantitative Analysis of Gene Expression
Authors: Li Hairi , Qiu Jinsong , Fu Xiang‐Dong 
Published: April 2012

AbstractLarge‐scale, quantitative analysis of gene expression can be accomplished by microarray or RNA‐seq analysis. While these methods are applicable to genome‐wide analysis, it is often desirable to quantify expression of a more limited set of genes in hundreds, thousands, or even tens of thousands of biological samples. For example, some studies may require monitoring a sizable panel of key genes under many different experimental conditions, during development, or following treatment with a large library of small molecules, for which current genome‐wide methods are either inefficient or cost‐prohibitive. This unit presents a method that permits quantitative profiling of several hundred selected genes in a large number of samples by coupling RNA‐mediated oligonucleotide Annealing, Selection, and Ligation with Next‐Gen sequencing (RASL‐seq). The method even allows direct analysis of RNA levels in cell lysates and is also adaptable to full automation, making it ideal for large‐scale analysis of multiple biological pathways or regulatory gene networks in the context of systematic genetic or chemical genetic perturbations. Curr. Protoc. Mol. Biol. 98:4.13.1‐4.13.9. © 2012 by John Wiley & Sons, Inc.
High‐Throughput Multiplex Sequencing of miRNA
Authors: Vigneault Francois , Ter‐Ovanesyan Dmitry , Alon Shahar , Eminaga Seda , C. Christodoulou Danos , Seidman J. G. , Eisenberg Eli , M. Church George 
Published: April 2012

AbstractNext‐generation sequencing offers many advantages over other methods of microRNA (miRNA) expression profiling, such as sample throughput and the capability to discover novel miRNAs. As the sequencing depth of current sequencing platforms exceeds what is necessary to quantify miRNAs, multiplexing several samples in one sequencing run offers a significant cost advantage. Although previous studies have achieved this goal by adding bar codes to miRNA libraries at the ligation step, this was recently shown to introduce significant bias into the miRNA expression data. This bias can be avoided, however, by bar coding the miRNA libraries at the PCR step instead. Here, we describe a user‐friendly PCR bar‐coding method of preparing multiplexed microRNA libraries for Illumina‐based sequencing. The method also prevents the production of adapter dimers and can be completed in one day. Curr. Protoc. Hum. Genet. 73:11.12.1‐11.12.10 © 2012 by John Wiley & Sons, Inc.
No evidence that major mtDNA European haplogroups confer risk to schizophrenia
Authors: Mosquera‐Miguel Ana , Torrell Helena , Abasolo Nerea , Arrojo Manuel , Paz Eduardo , Ramos‐Ríos Ramón , Agra Santiago , Páramo Mario , Brenlla Julio , Martínez Silvia , Vilella Elisabet , Valero Joaquín , Gutiérrez‐Zotes Alfonso , Martorell Lourdes , Costas Javier , Salas Antonio 
Published: April 2012

AbstractPrevious studies suggest that genetic factors could be involved in mitochondrial dysfunction observed in schizophrenia (SZ), some of them claiming a role of mtDNA common variants (mtSNPs) and/or haplogroups (hgs) in developing this disorder. These studies, however, have mainly been undertaken on relatively small cohorts of patients and control individuals and most have not yet been replicated. To further analyze the role of mtSNPs in SZ risk, we have carried out the largest genotyping effort to date using two Spanish case–control samples comprising a total of 942 schizophrenic patients and 1,231 unrelated controls: 454 patients and 616 controls from Santiago de Compostela (Galicia) and 488 patients and 615 controls from Reus (Catalonia). A set of 25 mtSNPs representing main branches of the European mtDNA phylogeny were genotyped in the Galician cohort and a subset of 16 out of these 25 mtSNPs was genotyped in the Catalan cohort. These 16 common variants characterize the most common European branches of the mtDNA phylogeny. We did not observe any positive association of mtSNPs and hgs with SZ. We discuss several deficiencies of previous studies that might explain the false positive nature of previous findings, including the confounding effect of population sub‐structure and deficient statistical methodologies. It is unlikely that mtSNPs defining the most common European mtDNA haplogroups are related to SZ. © 2012 Wiley Periodicals, Inc.
Automated methods for accurate determination of the critical velocity of packed bed chromatography
Authors: Chang Yu‐Chih , Gerontas Spyridon , Titchener‐Hooker Nigel J. 
Published: April 2012

AbstractKnowing the critical velocity (ucrit) of a chromatography column is an important part of process development as it allows the optimization of chromatographic flow conditions. The conventional flow step method for determining ucrit is prone to error as it depends heavily on human judgment. In this study, two automated methods for determining ucrit have been developed: the automatic flow step (AFS) method and the automatic pressure step (APS) method. In the AFS method, the column pressure drop is monitored upon application of automated incremental increases in flow velocity, whereas in the APS method the flow velocity is monitored upon application of automated incremental increases in pressure drop. The APS method emerged as the one with the higher levels of accuracy, efficiency and ease of application having the greater potential to assist defining the best operational parameters of a chromatography column. © 2012 American Institute of Chemical Engineers Biotechnol. Prog., 2012
Flow cytometry assessment of residual melanoma cells in tumor‐infiltrating lymphocyte cultures
Authors: Richards John O. , Treisman Jonathan , Garlie Nina , Hanson John P. , Oaks Martin K. 
Published: April 2012

AbstractAdoptive transfer of tumor‐infiltrating lymphocytes (TIL) is in development for the treatment of metastatic melanoma. In phase II clinical trials, patients with metastatic melanoma that received TIL after preconditioning had a 50–70% clinical response rate. The current approach to generate TIL is to culture melanoma enzyme digests in the presence of IL‐2 for a 10‐ to 20‐day period followed by 2 weeks of rapid expansion (REP). Prior to administration, cell therapies are characterized and tested for purity. TIL are characterized by CD3 surface marker expression, and purity is assessed by the amount of tumor remaining in culture. Evaluating TIL purity has traditionally been done by immunohistochemistry, which is often considered semiquantitative. To generate a quantitative assay, we used multiparameter flow cytometry to evaluate the presence of viable tumor cells by staining TIL populations with a viability dye and an antibody cocktail that detects intracellular tumor‐antigens gp100, Mart‐1, tyrosinase, S100, and surface tumor‐antigen melanoma chondroitin sulfate proteoglycan (MCSP), and CD3 on T cells. Tumors were identified by gating on the viable CD3− population. Antigens in tumors were initially optimized with individual antibodies using both immunohistochemistry and flow cytometry. When eight different tumor cell lines were spiked into an activated T cell culture, flow cytometry was able to distinguish lymphocytes from tumors in all samples tested. Most importantly, the assay was able to detect melanoma cells in all enzyme digests (9/9) from patient samples. After IL‐2‐induced TIL expansion, there was a significant decrease in tumor cells; tumor cells were detected in only 2 of 12 samples. In eight IL‐2‐induced TIL samples that were further expanded in REP, no tumor cells were detected. We have demonstrated that flow cytometry is an alternative to immunohistochemistry for defining the purity of a TIL population. © 2012 International Society for Advancement of Cytometry
The relationship between environmental management systems and organizational innovations
Authors: Llach Josep , Castro Rodolfo de , Bikfalvi Andrea , Marimon Frederic 
Published: April 2012

AbstractThis study provides empirical evidence of the nature of the relationship between environmental management systems and organizational innovations. Using a sample of Spanish manufacturing firms, the study reports on the implementation of the International Organization for Standardization (ISO) 14000 environmental quality standards and the relationship of such implementation with organizational innovations in the firm. The study presents several implications of interest for practitioners, academics, and policy makers. © 2012 Wiley Periodicals, Inc.
Mechanism of oxide layer growth during annealing of NiTi
Authors: Undisz Andreas , Schrempel Frank , Wesch Werner , Rettenmayr Markus 
Published: April 2012

AbstractAfter annealing at 540°C, NiTi is covered by a characteristic oxide layer with an Ni‐containing outer and an Ni‐free inner titanium oxide region. To elucidate details of the yet unclear formation process, samples were annealed in an atmosphere containing different oxygen isotopes at a time and analyzed by nondestructive ion beam techniques at different stages of the oxidation. During the heating stage, an oxygen permeable “low Ni” titanium oxide forms, and the oxide layer grows inward. Subsequently, when the annealing temperature of 540°C is reached, Ni‐free stoichiometric titanium oxide forms and inhibits the transport of oxygen toward the bulk. Thus, the oxide layer growth changes to outward, and the final location of the reaction front between O and Ti is inside the oxide layer at the transition of “low Ni” oxide to “Ni‐free” oxide. Consequently, the annealing conditions during inward oxide layer growth govern the surface properties, whereas the conditions during outward oxide layer growth are uncritical with respect to the surface properties. The findings are directly applicable to set the amount of surface Ni of NiTi devices, provide basis for detailed interpretation of experimental results involving annealing of NiTi, and can further respective modeling. © 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.
Neural Stem Cells Directly Differentiated from Partially Reprogrammed Fibroblasts Rapidly Acquire Gliogenic Competency
Authors: Matsui Takeshi , Takano Morito , Yoshida Kenji , Ono Soichiro , Fujisaki Chikako , Matsuzaki Yumi , Toyama Yoshiaki , Nakamura Masaya , Okano Hideyuki , Akamatsu Wado 
Published: April 2012

AbstractNeural stem cells (NSCs) were directly induced from mouse fibroblasts using four reprogramming factors (Oct4, Sox2, Klf4, and cMyc) without the clonal isolation of induced pluripotent stem cells (iPSCs). These NSCs gave rise to both neurons and glial cells even at early passages, while early NSCs derived from clonal embryonic stem cells (ESCs)/iPSCs differentiated mainly into neurons. Epidermal growth factor-dependent neurosphere cultivation efficiently propagated these gliogenic NSCs and eliminated residual pluripotent cells that could form teratomas in vivo. We concluded that these directly induced NSCs were derived from partially reprogrammed cells, because dissociated ESCs/iPSCs did not form neurospheres in this culture condition. These NSCs differentiated into both neurons and glial cells in vivo after being transplanted intracranially into mouse striatum. NSCs could also be directly induced from adult human fibroblasts. The direct differentiation of partially reprogrammed cells may be useful for rapidly preparing NSCs with a strongly reduced propensity for tumorigenesis.Disclosure of potential conflicts of interest is found at the end of this article.
Two 2D Metal‐organic Networks Based on a Rigid Imidazolate/Sulfonate Functionalized Ligand – Effect of the Coordination Modes of the Ligand on Crystal Structures
Authors: Wang Xiu‐Ying , Xu Long , Liu Rui‐Bin , Liu Shu‐Qin , Yang Wen‐Bo , Zhang Jian‐Jun 
Published: April 2012

AbstractA rigid imidazolate/sulfonate functionalized ligand, 6‐(4‐sulfonatopheny)imidazo[4, 5‐f]isoindole‐5, 7‐dione (SPID) was designed and used for assembling reactions with Mn2+ and Cu2+ ions. Two 2D frameworks compounds, [Mn(H‐1SPID)2(DMAC)2] (1) and [Cu(H‐2SPID)(H2O)2]·0.7DMAC·0.3H2O (2) (DMAC = N,N‐dimethylacetamide) were obtained. Single crystal X‐ray analyses show that 1 has a 2D (4, 4)‐net based on 4‐connected Mn2+ nodes and μ2‐coordinated H‐1SPID spacers, whereas compound 2 has a 2D (6, 3)‐net built of 3‐connected Cu2+ nodes and μ3‐coordinated H‐2SPID spacers. Additionally, the thermal behavior of 1 and 2 is presented.
Synthesis, Characterization, and Structures of Palladium(II) and Platinum(II) Complexes Containing N,N‐Bis(diphenylphos­phanyl)Naphthylamine
Authors: Al‐Masri Harbi Tomah 
Published: April 2012

AbstractThe reaction of 1‐naphthylamine with two equivalents of chlorodiphenylphosphine in the presence of triethylamine gave the ligand C10H7‐1‐N(PPh2)2 (1). Reaction of 1 with PdCl2(CH3CN)2 or PtCl2(cod) (1:1 molar ratio) afforded the complexes cis‐[PdCl2{C10H7‐1‐N(PPh2)2}] (2) and cis‐[PtCl2{C10H7‐1‐N(PPh2)2}] (3), respectively. Compounds 1–3 were identified and characterized by multinuclear NMR (1H, 13C, 31P NMR) and IR spectroscopy. Crystal structure determinations of complexes 2 and 3 were carried out.