bluuebunny/crossref_metadata_2025_split huggingface dataset:
VEGF‐A expression in osteoclasts is regulated by NF‐κB induction of HIF‐1α
Authors: Trebec‐Reynolds Diana P. , Voronov Irina , Heersche Johan N.M. , Manolson Morris F. 
Published: April 2010

AbstractLarge osteoclasts (10+ nuclei), predominant in rheumatoid arthritis and periodontal disease, have higher expression of proteases and activating receptors and also have increased resorptive activity when compared to small (2–5 nuclei) osteoclasts. We hypothesized that large and small osteoclasts activate different signaling pathways. A Signal Transduction Pathway Finder Array™ was used to compare gene expression of large and small osteoclasts in RAW 264.7‐derived osteoclasts. Expression of vascular endothelial growth factor A (Vegfa) was higher in large osteoclasts and this result was confirmed by RT‐PCR. RT‐PCR further showed that RANKL treatment of RAW cells induced Vegfa expression in a time‐dependent manner. Moreover, VEGF‐A secretion in conditioned media was also increased in cultures with a higher proportion of large osteoclasts. To investigate the mechanism of Vegfa induction, specific inhibitors for the transcription factors NF‐κB, AP‐1, NFATc1, and HIF‐1 were used. Dimethyl bisphenol A, the HIF‐1α inhibitor, decreased Vegfa mRNA expression, whereas blocking NF‐κB, AP‐1, and NFATc1 had no effect. Furthermore, the NF‐κB inhibitor gliotoxin inhibited Hif1α mRNA expression. In conclusion, VEGF‐A gene and protein expression are elevated in large osteoclasts compared to small osteoclasts and this increase is regulated by HIF‐1. In turn, Hif1α mRNA levels are induced by RANKL‐mediated activation of NF‐κB. These findings reveal further differences in signaling between large and small osteoclasts and thereby identify novel therapeutic targets for highly resorptive osteoclasts in inflammatory bone loss. J. Cell. Biochem. 110: 343–351, 2010. © 2010 Wiley‐Liss, Inc.
DNA damage during mitosis invokes a JNK‐mediated stress response that leads to cell death
Authors: Ho Chin‐Yee , Li Hoi‐Yeung 
Published: April 2010

AbstractMitotic catastrophe is a phenomenon displayed by cells undergoing aberrant mitosis to eliminate cells that fail to repair the errors. Why and how mitotic catastrophe would lead to cell death remains to be resolved and the answer will prove valuable in design of better therapeutic agents that specifically target such cells in mitosis. The antibiotic actinomycin D has been shown to induce chromosomal lesions in lower order organisms as well as in human interphase cells. Relatively few studies have been conducted to elucidate molecular events in the context of mitotic DNA damage. We have previously established a model of mitotic catastrophe in human HeLa cells induced by actinomycin D. Here, we show that actinomycin D induce cellular stress via DNA damage during mitosis. The higher order packing of chromosomes during mitosis might impede efficient DNA repair. γH2AX serves as a marker for DNA repair and active JNK interacts with γH2AX in actinomycin D‐treated mitotic extracts. We believe JNK might be in part, responsible for the phosphorylation of H2AX and thereby, facilitate the propagation of a positive signal for cell death, when repair is not achieved. The mitotic cell activates JNK‐mediated cell death response that progresses through a caspase cascade downstream of the mitochondria. In the mean time, remaining checkpoint signals may be sufficient to put a restraining hand on entry into anaphase and the cell eventually dies in mitosis. J. Cell. Biochem. 110: 725–731, 2010. © 2010 Wiley‐Liss, Inc.
TRAF6 is autoinhibited by an intramolecular interaction which is counteracted by trans‐ubiquitination
Authors: Wang Kent Z.Q. , Galson Deborah L. , Auron Philip E. 
Published: April 2010

AbstractThe tumor necrosis factor (TNF) receptor associated factor (TRAF) class of intracellular signal transducers is responsible for mediating many of the activation events initiated by TNF receptor (TNFR) and Toll‐like/Interleukin‐1, ‐17, and ‐18 receptor (TIR) families. Investigation of the mechanism by which TRAF6 is activated has demonstrated that two critical domains of the molecule required for activation and downstream signaling are involved in an interaction which renders the molecule inactive and structurally closed, as well as incapable of auto‐ubiquitination. Contrary to its assumed role as a direct mediator of protein–protein interaction, TRAF auto‐ubiquitination is a means of sustaining an open conformation active in downstream signaling. Furthermore, the inferred cis‐function of TRAF auto‐ubiquitination is now demonstrated to act in trans and requires both the RING‐Zinc (RZ) fingers region and coiled‐coil domain. We also observed that both the RZ fingers region and the MATH domain are targets for ubiquitination. Although TRAF6 ubiquitination has emerged as a hallmark of activation, trans‐ubiquitination induced by two TRAF6 muteins is insufficient for NF‐κB activation. J. Cell. Biochem. 110: 763–771, 2010. © 2010 Wiley‐Liss, Inc.
Human resource challenges for small growing companies in Flanders
Authors: Van De Woestyne Mieke , Dewettinck Koen , Van Bruystegem Kristien 
Published: April 2010

AbstractFor Flemish entrepreneurs, human resource management (HRM) is one of the biggest challenges for further development and growth. Hence, we aim to shed light on how successful entrepreneurs in Flanders manage their human resources. Building on the growth models of Greiner and Ulrich, we conducted a qualitative study of various human resource practices within Flemish entrepreneurial firms in different sectors and stages of growth. We investigated the interplay between the focus of HRM and the growth phase of a small or medium‐sized enterprise (SME). The main objective is to better understand the major challenges entrepreneurs experience when managing people during the first stages of company growth. Our findings suggest that HRM within growing SMEs shifts from an operational focus on people to a more strategic focus on procedures. Throughout the process of evolution, entrepreneurs need to find the right balance of HR practices, paying attention to formalization, delegation, and coaching. © 2010 Wiley Periodicals, Inc.
F‐spondin regulates chondrocyte terminal differentiation and endochondral bone formation
Authors: Palmer Glyn D. , Piton Alejandro H. , Thant Lwin Mon , Oliveira Serafim M. , D'Angelo Marina , Attur Mukundan G. , Abramson Steven B. , Teixeira Cristina C. 
Published: April 2010

AbstractThis study examines the role of F‐spondin, an extracellular matrix protein of osteoarthritic cartilage, during chondrocyte maturation in embryonic growth plate cartilage. In chick tibia, F‐spondin expression localized to the hypertrophic and calcified zones of the growth plate. Functional studies using tibial organ cultures indicated that F‐spondin inhibited (∼35%, p = 0.02), and antibodies to F‐spondin increased (∼30%, p < 0.1) longitudinal limb growth relative to untreated controls. In cell cultures, induction of chondrocyte maturation, by retinoic acid (RA) or transforming growth factor (TGF)‐β treatment led to a significant upregulation of F‐spondin (p < 0.05). F‐spondin transfection increased mineral deposition, alkaline phosphatase (AP) and matrix metalloproteinase (MMP)‐13 mRNA levels (p < 0.05), and AP activity following RA stimulation, compared to mock transfected controls. Using AP as a differentiation marker we then investigated the mechanism of F‐spondin promaturation effects. Blocking endogenous F‐spondin via its thrombospondin (TSR) domain inhibited RA induced AP activity 40% compared to controls (p < 0.05). The stimulatory effect of F‐spondin on AP expression was also inhibited following depletion of TGF‐β from culture supernatants. Our findings indicate that F‐spondin is expressed in embryonic cartilage, where it has the capacity to enhance chondrocyte terminal differentiation and mineralization via interactions in its TSR domain and TGF‐β dependent pathways. Published by Wiley Periodicals, Inc. J Orthop Res 28:1323–1329, 2010
Cholesterol crystal embolization presenting as either solid or cystic pancreatic lesion
Authors: Konstantinidis Ioannis T. , Warshaw Andrew L. , Deshpande Vikram , Sahani Dushyant , Berger David , Castillo Carlos Fernandez‐del , Ferrone Cristina R. 
Published: April 2010

AbstractCholesterol crystal embolization (CCE) can result in end‐organ ischemia. Retrospective review of clinicopathologic data. The first patient was anticoagulated with coumadin for chronic atrial fibrillation and presented subacutely with a solid mass. The second patient suffered from coronary artery disease post‐angioplasty/stenting and presented with acute pancreatitis and pancreatic cystification. CCE should be considered in patients with significant vascular disease, arrhythmias or vascular manipulation who present with a pancreatic mass. J. Surg. Oncol. 2010;102:706–708. © 2010 Wiley‐Liss, Inc.
The parasympathetic supply to the distal colon—one marker for precisely locating the posterior dissection plane in the operation of TME
Authors: Dong‐song Bi , Tao Li , Ke‐xin Wang , Hai‐dong Gao , San‐yuan Hu 
Published: April 2010

AbstractBackgroundIt is important for surgeons to locate the reliable surgical planes in the operation of total mesorectal excision (TME); we observe the parasympathetic nerve to the distal colon can be served as one of useful markers for precisely locating the posterior dissection plane in TME.Materials and MethodsFrom October 2006 to January 2008, 26 patients underwent TME for rectal cancer. The dissections of the parasympathetic nerves to the distal colon were performed and the relationship of these nerves to the prehypogastric nerve fascia was observed.ResultsSome parasympathetic nerves ran upwards and lay anteromedial to the hypogastric nerves. In the avascular space between prehypogastric nerve fascia and the fascia propria of the rectum, the prehypogastric nerve fascia enveloped parasymphathetic nerve up to the fascia propria of rectum.ConclusionsThe parasympathetic nerve to the distal colon is evident between the fascia propria of the rectum and the prehypogastric nerve fascia. As the precise dissection plane of TME lay between the fascia propria of the rectum and the prehypogastric nerve fascia, these nerves could be served as useful marker for precisely locating the posterior dissection plane in TME. J. Surg. Oncol. 2010; 101:524–526. © 2010 Wiley‐Liss, Inc.
ALCAM is associated with chemoresistance and tumor cell adhesion in pancreatic cancer
Authors: Hong Xin , Michalski Christoph W. , Kong Bo , Zhang WeiWei , Raggi Matthias C. , Sauliunaite Danguole , De Oliveira Tiago , Friess Helmut , Kleeff Jörg 
Published: April 2010

AbstractAimsCell–cell adhesion is a major factor in integrity of epithelia which is frequently disturbed in cancer leading to local invasion and distant metastasis.MethodsTo define expression and function of activated leukocyte cell adhesion molecule (ALCAM, CD166) in pancreatic cancer and in pancreatic neuroendocrine tumors (PNET), microarray analyses, RT‐PCR, immunohistochemistry, RNAi, adhesion, migration, invasion, and chemoresistance assays were used.ResultsWe demonstrate that expression of ALCAM is altered and its serum levels are increased in pancreatic ductal adenocarcinoma (PDAC). ALCAM was expressed on the membranes of islet cells in the normal pancreas whereas normal pancreatic ducts were ALCAM‐negative. In PDAC, ALCAM expression was generally rare though in some tumors, membranous, or cytoplasmic ALCAM was found. PNET were mostly ALCAM‐positive with a cytoplasmic staining pattern which was in contrast to the membrane expression observed in non‐transformed islet cells. In vitro, ALCAM silencing using RNAi had no effects on growth or invasion of pancreatic cancer cells but reduced cell adhesion and induced chemoresistance. In neuroendocrine tumor cell lines, silencing of ALCAM decreased cell growth.ConclusionsWe propose ALCAM as a novel serum biomarker in human pancreatic tumors which is associated with cell adhesion, growth and chemoresistance. J. Surg. Oncol. 2010; 101:564–569. © 2010 Wiley‐Liss, Inc.
Case‐matched analysis of totally laparoscopic versus open liver resection for HCC: Short and middle term results
Authors: Aldrighetti Luca , Guzzetti Eleonora , Pulitanò Carlo , Cipriani Federica , Catena Marco , Paganelli Michele , Ferla Gianfranco 
Published: April 2010

AbstractBackground and ObjectivesLaparoscopy is gaining acceptance as a safe procedure for resection of liver neoplasms. The aim of this study is to evaluate surgical results and mid‐term survival of minor hepatic resection performed for HCC.MethodsData of 16 patients with HCC, undergoing laparoscopic hepatectomy from September 2005 to January 2009, were compared to a control group of 16 patients who underwent open resection (OR) during the same period. The two groups were matched in terms of type of resection, tumor size, and severity of cirrhosis.ResultsOne patient underwent conversion to an open approach. Laparoscopic approach resulted in shorter operating time (150 min, P:0.044) and lower blood loss (258 ml, P:0.008). There was no difference in perioperative morbidity and mortality rate; laparoscopic approach was associated with a shorter hospital stay (6.3 days, P:0.039). After a mean follow up of 32 months, disease free survival and overall survival were 40.2 and 23.3 months for laparoscopic group, and 47.7 and 31.4 months for OR group (P NS).ConclusionLaparoscopic resection of HCC is feasible and safe in selected patients and can result in good surgical results, with similar outcomes in terms of overall and disease‐free survival. J. Surg. Oncol. J. Surg. Oncol. 2010;102:82–86. © 2010 Wiley‐Liss, Inc.
Adjuvant chemoradiation therapy in gallbladder cancer
Authors: Cho Seong Yeon , Kim Seong Hoon , Park Sang‐Jae , Han Sung‐Sik , Kim Young‐Kyu , Lee Kwang‐Woong , Lee Woo Jin , Woo Sang Myung , Kim Tae‐Hyun 
Published: April 2010

AbstractBackground and ObjectivesGallbladder cancer is a relatively uncommon gastrointestinal malgnancy. Indications for adjuvant chemoradiation therapy after surgical resection have not yet been determined. We aimed this study to elucidate the effectiveness of adjuvant chemoradiation therapy according to TNM stage for gallbladder cancer.MethodsBetween March 2001 and March 2009, 100 patients with gallbladder cancer underwent surgical resection. We divided the patients according to TNM stage, and subdivided further according to whether adjuvant chemoradiation therapy was added or not. The clinicopathologic factors, recurrence and survival were retrospectively analyzed.ResultsPatients with gallbladder cancer at T2N0M0, T2N1M0, T3N0M0, and T3N1M0 stages were enrolled in this study. Among the four stages, the two lymph node‐negative stages (T2N0M0 and T3N0M0) did not show any gain in survival by adding adjuvant chemoradiation therapy. Conversely, the remaining lymph node‐positive stages (T2N1M0 and T3N1M0) showed gain in disease‐free survival, and the lymph node‐positive T2 stage (T2N1M0) showed gain in disease‐specific survival. In patients with lymph node‐positive T2/T3 GB cancers, adjuvant chemoradiation therapy was an independent prognostic factor for survival.ConclusionsAdjuvant chemoradiation therapy is recommended for lymph node‐positive T2/T3 gallbladder cancer following surgical resection. J. Surg. Oncol. J. Surg. Oncol. 2010;102:87–93. © 2010 Wiley‐Liss, Inc.
Analysis of esophageal reflux after proximal gastrectomy measured by wireless ambulatory 24‐hr esophageal pH monitoring and TC‐99m diisopropyliminodiacetic acid (DISIDA) scan
Authors: Kim Jong Won , Yoon Hongman , Kong Seong‐Ho , Kim Joo‐Sung , Paeng Jin Chul , Lee Hyuk‐Joon , Lee Kuhn Uk , Yang Han‐Kwang 
Published: April 2010

AbstractBackground and objectivesReflux is one of the most common sequela after proximal gastrectomy (PG). The aim of this study was to find a predicting factor related to the character of esophageal reflux after PG.MethodsWireless ambulatory 24‐hr pH monitoring (for acid reflux, AR) and diisopropyliminodiacetic acid hepatobiliary scan (for bile reflux, BR) were performed on 24 patients who had reflux symptoms after PG with esophagogastrostomy from July 2008 to March 2009. Endoscopic examination was done and the length of remnant stomach (LoRS) was measure by postoperative UGI series.ResultsEleven patients (45.8%) had only BR, 7 (29.2%) had AR only, Two patients (8.3%) had both acid and BR, and 3 (12.5%) had neither. The LoRS along greater curvature was significantly shorter in patients with only BR (16.11 ± 2.87 cm) than in patients with only AR (23.69 ± 6.15 cm, P = 0.003). Severity of symptoms or esophagitis was not significantly correlated with the content of acid or BR.ConclusionReflux symptom after PG is caused by either bile or acid rather than both. Character of reflux was related to the LoRS. J. Surg. Oncol. 2010; 101:626–633. © 2010 Wiley‐Liss, Inc.
Zeta potential determination as a new way of stationary phases characterization for liquid chromatography
Authors: Buszewski Bogusław , Bocian Szymon , Dziubakiewicz Ewelina 
Published: April 2010

AbstractA set of seven homemade octadecyl silica‐based bonded phases was investigated. Their zeta potential data in methanol and ACN as well as in methanol–water and ACN–water solution were obtained using Zetasizer. The influence of both the coverage density of bonded ligands and the end‐capping of the modified surface on these data was investigated. Presented results may give useful information about the accessibility of the residual silanols in different mobile phases during the chromatographic analysis. Those measurements may be useful to choose chemically bonded stationary phases for CEC. The results also confirm the phenomena of anion exclusion from the pores of stationary‐bonded phase.
Highly sensitive determination of tetrabromobisphenol A and bisphenol A in environmental water samples by solid‐phase extraction and liquid chromatography‐tandem mass spectrometry
Authors: Zhao Ru‐Song , Wang Xia , Yuan Jin‐Peng 
Published: April 2010

AbstractUsing bamboo‐activated charcoal as SPE adsorbent, a novel SPE method was developed for the sensitive determination of tetrabromobisphenol A and bisphenol A in environmental water samples by rapid‐resolution LC‐ESI‐MS/MS. Important parameters influencing extraction efficiency, including type of eluent, eluent volume, sample pH, volume and flow rate, were investigated and optimized. Under the optimal extraction conditions (eluent: 8 mL methanol, pH: 7; flow rate: 4 mL/min; sample volume: 100 mL), low LODs (0.01–0.02 ng/mL), good repeatability (6.2–8.3%) and wide linearity range (0.10–10 ng/mL) were obtained. Satisfied results were achieved when the proposed method was applied to determine the two target compounds in real‐world environmental water samples with spiked recoveries over the range of 80.5–119.8%. All these facts indicate that trace determination of tetrabromobisphenol A and bisphenol A in real‐world environmental water samples can be realized by bamboo‐activated charcoal SPE‐rapid resolution‐LC‐ESI‐MS/MS.
Preparing titania aerogel monolithic chromatography columns using supercritical carbon dioxide
Authors: Sui Ruohong , Liu Suya , Lajoie Gilles A. , Charpentier Paul A. 
Published: April 2010

AbstractThe search for a method to fabricate monolithic inorganic columns has attracted significant recent attention due to their unique ability in separation applications of various biomolecules. Silica and polymer based monolithic columns have been prepared, but titania and other metal oxide monoliths have been elusive, primarily due to their fragility. This article describes a new approach for preparing nanostructured titania based columns, which offer better performance over conventional particle packed columns for separating a wide variety of biomolecules including phosphopeptides. TiO2 monolithic aerogels were synthesized in separation columns using in situ sol‐gel reactions in supercritical carbon dioxide (scCO2) followed by calcination, and compared to those prepared in heptanes. The characterization results show that scCO2 is a better solvent for the sol‐gel reactions, providing lower shrinkage with the anatase TiO2 monolith composed of nanofibers with very high surface areas. The monolithic columns show the ability to isolate phosphopeptides with little flow resistance compared to conventional titania particle based microcolumns.
Effect of porosity of phosphate coating on corrosion resistance of galvanized and phosphated steels Part I: Measurement of porosity of phosphate
Authors: Teixeira C. H. S. B. , Alvarenga E. A. , Vasconcelos W. L. , Lins V. F. C. 
Published: April 2010

AbstractThe corrosion resistance of phosphated and painted steels is associated with the integrity of phosphate and paint layers. In this work, a methodology to measure the porosity of phosphated coatings based on cathodic polarization technique with an electrolyte of a sodium sulfate aqueous solution was developed. The morphology and mass of phosphate layers were determined. Phosphating was performed using the spraying and immersion techniques, varying the refiner content of bath in the immersion operation. The phosphate coatings obtained by immersion with a refiner showed the lowest values of porosities among the samples studied. The phosphate layers, obtained by spraying, immersion without a refiner, and immersion with a half content of refiner, showed the lowest porosity on electrogalvanized steel. The phosphate layer obtained by immersion with a refiner displayed the lowest porosity on the substrate of ungalvanized steel.
Comparative analysis for the corrosion susceptibility of Cu, Al, Al–Cu and C‐steel in soil solution
Authors: Noor E. A. 
Published: April 2010

AbstractCorrosion of different materials (Cu, Al, Al–Cu and C‐steel) in soil solution was evaluated at ambient temperature using various techniques. The weight loss of the studied materials varied exponentially with time. Kinetic data revealed that the corrosion of Cu and Al–Cu are diffusion controlled while Al and C‐steel show gradual change from diffusion control to charge transfer control. Nonferrous materials have better durability factors than C‐steel. Good consistency between the data obtained from EIS and PDP measurements was observed. SEM showed a biogenic corrosion layer with density that depends on the metal type. Correlation between corrosion rates and the estimated soil solution corrosivity was obtained.
Corrosion of API XL 52 steel in presence of Clostridium celerecrescens
Authors: Monroy O. A. Ramos , Gayosso M. J. Hernández , Ordaz N. Ruiz , Olivares G. Zavala , Ramírez C. Juárez 
Published: April 2010

AbstractDuring the characterization of sediments formed in pipelines transporting hydrocarbons, the knowledge of the microbiological diversity becomes very interesting, especially when it is related to microbiologically influenced corrosion (MIC). The presence of microorganisms is considered as one of the factors that affect the corrosion processes occurring at the pipeline; therefore, their corrosiveness must be determined. In this way, the identification of new species affecting the MIC processes is still considered relevant. In this work, the effect of Clostridium celerecrescens upon the corrosion of API KL 52 steel was evaluated. This microorganism was isolated and identified from the sediments collected during the inner cleaning procedures of a gas pipeline. The polarization resistance (PR) and electrochemical impedance spectroscopy (EIS) techniques were considered to estimate the microorganism behavior during the corrosion process. The results were complemented with a metal surface analysis, using a scanning electron microscope (SEM). The resistance values induced by the presence of the microorganisms clearly indicated that C. celerecrescens has an effect on the corrosion process occurring at the API XL 52 steel surface.