bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhanced local dendritic cell activity and tumor‐specific immunoresponse in combined radiofrequency ablation and interleukin‐2 for the treatment of human head and neck cancer in a murine orthotopic model
Authors: Saito Koichiro , Araki Koji , Reddy Nishant , Guang Wei , O'Malley Bert W. , Li Daqing 
Published: July 2010

AbstractBackground.Radiofrequency ablation (RFA) is a minimally invasive tumor destruction technique and can provide the antigen source initiating tumor immunity. However, induced immune response is weak and requires additional immunotherapy for optimized RFA treatment against cancer.Methods.A murine orthotopic model of head and neck squamous cell carcinoma (HNSCC) was established to investigate the efficacy and mechanism of an RFA + interleukin‐2 (IL‐2) combination adenoviral gene therapy among 6 groups. Tumor volume change, apoptosis, in situ macrophage recruitment, cytotoxic T lymphocyte (CTL) activity, migration of dendritic cells into the regional lymph nodes, and systemic antitumor immunity were examined.Results.RFA + IL‐2 therapy induced the highest levels of macrophage recruitment and dendritic cell migration resulting in enhanced CTL activity, increased tumor apoptosis, enhanced systemic antitumor immunity, and the best inhibition of tumor growth among all groups.Conclusion.RFA + IL‐2 combination therapy generates potent tumor immunity to suppress tumor growth in HNSCC. © 2010 Wiley Periodicals, Inc. Head Neck, 2010
Alteration of microRNA profiles in squamous cell carcinoma of the head and neck cell lines by human papillomavirus
Authors: Wald Abigail I. , Hoskins Elizabeth E. , Wells Susanne I. , Ferris Robert L. , Khan Saleem A. 
Published: July 2010

AbstractBackgroundHuman papillomavirus (HPV)‐positive cases of squamous cell carcinoma of the head and neck (SCCHN) have a much better disease outcome compared to SCCHN cases lacking HPV. Differences in microRNA (miRNA) expression may affect their clinical outcomes.MethodsThe miRNA expression was studied using microarrays and quantitative reverse transcriptase‐polymerase chain reaction (RT‐PCR) in HPV‐16‐positive and HPV‐negative SCCHN cell lines. The role of HPV‐16 E6 and E7 oncogenes in altering miRNA expression was investigated using human foreskin keratinocytes (HFKs).ResultsThe miRNAs miR‐363, miR‐33, and miR‐497 were upregulated, whereas miR‐155, miR‐181a, miR‐181b, miR‐29a, miR‐218, miR‐222, miR‐221, and miR‐142‐5p were downregulated in HPV‐positive cells compared to both HPV‐negative SCCHN and normal oral keratinocytes. HPV‐16 E6 oncogene altered miRNA expression in HFKs and in an HPV‐16–positive cell line with E6 knockdown using siRNA.ConclusionmiRNAs differentially expressed in the presence of HPV‐16 may provide biomarkers for SCCHN and identify cellular pathways targeted by this virus. © 2010 Wiley Periodicals, Inc. Head Neck, 2011
Estimation of nonlinear models with mismeasured regressors using marginal information
Authors: Hu Yingyao , Ridder Geert 
Published: July 2010

AbstractWe consider the estimation of nonlinear models with mismeasured explanatory variables, when information on the marginal distribution of the true values of these variables is available. We derive a semi‐parametric MLE that is shown to be$\sqrt{n}$consistent and asymptotically normally distributed. In a simulation experiment we find that the finite sample distribution of the estimator is close to the asymptotic approximation. The semi‐parametric MLE is applied to a duration model for AFDC welfare spells with misreported welfare benefits. The marginal distribution of the correctly measured welfare benefits is obtained from an administrative source. Copyright © 2010 John Wiley & Sons, Ltd.
A CAS study on S‐loss and O‐loss dissociation mechanisms of the SO2+ ion in the C, D, and E states
Authors: Meng Qingyong , Huang Ming‐Bao 
Published: July 2010

AbstractIn the present work, we mainly study dissociation of the C
2B1, D2A1, and E2B2 states of the SO2+ ion using the complete active‐space self‐consistent field (CASSCF) and multiconfiguration second‐order perturbation theory (CASPT2) methods. We first performed CASPT2 potential energy curve (PEC) calculations for S‐ and O‐loss dissociation from the X, A, B, C, D, and E primarily ionization states and many quartet states. For studying S‐loss predissociation of the C, D, and E states by the quartet states to the first, second, and third S‐loss dissociation limits, the CASSCF minimum energy crossing point (MECP) calculations for the doublet/quartet state pairs were performed, and then the CASPT2 energies and CASSCF spin‐orbit couplings were calculated at the MECPs. Our calculations predict eight S‐loss predissociation processes (via MECPs and transition states) for the C, D, and E states and the energetics for these processes are reported. This study indicates that the C and D states can adiabatically dissociate to the first O‐loss dissociation limit. Our calculations (PEC and MECP) predict a predissociation process for the E state to the first O‐loss limit. Our calculations also predict that the E2B2 state could dissociate to the first S‐ and O‐loss limits via the A2B2 ← E2B2 transition. On the basis of the 13 predicted processes, we discussed the S‐ and O‐loss dissociation mechanisms of the C, D, and E states proposed in the previous experimental studies. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2010
Supersecondary structure prediction using Chou's pseudo amino acid composition
Authors: Zou Dongsheng , He Zhongshi , He Jingyuan , Xia Yuxian 
Published: July 2010

AbstractSupersecondary structures (SSSs) are the building blocks of protein 3D structures. Accurate prediction of SSSs can be one important step toward building a tertiary structure from the specified secondary structure. How to improve the accuracy of prediction of SSSs by effectively incorporating the sequence order effects is an important and challenging problem. Based on a different form of Chou's pseudo amino acid composition, a novel approach for feature representation of SSSs is proposed. Amino acid basic compositions, dipeptide components, and amino acid composition distribution are incorporated to represent the compositional features of proteins. Each supersecondary structural motif is characterized as a vector of 36 dimensions. In addition, we propose a novel prediction system by using SVM and IDQD algorithm as classifiers. Our method is trained and tested on ArchDB40 dataset containing 3088 proteins. The highest overall accuracy for the training dataset and the independent testing dataset are 77.7 and 69.4%, respectively. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2011
Reply to the comment on “A stationary‐wave model of enzyme catalysis”
Authors: Canepa Carlo 
Published: July 2010

AbstractThis letter briefly outlines the grounds that led to an expression for reaction rate co‐efficients based on the incomplete gamma function of Euler. The proposed law explains the catalytic activity of enzymes as an effect of the coupling of active vibrational modes to the reaction coordinate, and predicts the observed negative curvature of the Arrhenius plots of the relevent reactions. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2011
Comment on “A stationary‐wave model of enzyme catalysis” by Carlo Canepa
Authors: Lonsdale Richard , Harvey Jeremy N. , Manby Frederick R. , Mulholland Adrian J. 
Published: July 2010

AbstractEnergy barriers for enzyme‐catalyzed reactions calculated with quantum mechanics/molecular mechanics (QM/MM) and empirical valence bond (EVB) methods can be in excellent agreement with activation energies derived from experiments, supporting the applicability of transition state theory for enzymic reactions. © 2010 Wiley Periodicals, Inc. J Comput Chem, 2011
The influence of hydrating agents on the hydration of industrial magnesium oxide
Authors: Matabola Kgabo P , van der Merwe Elizabet M , Strydom Christien A , Labuschagne Frederick J W 
Published: July 2010

AbstractBACKGROUND: The influence of different hydrating agents on the pH of the hydrating solutions, rate of hydration of MgO to Mg(OH)2 and product surface area was studied as a function of temperature of hydration. Hydrating agents used were aqueous solutions of ammonium chloride, magnesium acetate, magnesium nitrate, nitric acid, acetic acid, magnesium chloride, sodium acetate and hydrochloric acid and distilled water as control. These were chosen to determine either the effect of addition of a common ion, the effect of changing the solution pH or due to the presence of an acetate ion, found earlier to have a beneficial effect on the hydration of MgO.RESULTS: There was no significant difference in the hydration behaviour of the hydrating agents up to 50 °C, where less than 10% of magnesium hydroxide was formed. The amount of hydroxide increased at temperatures above 60 °C. When compared with the hydration in water, all the hydrating agents, with the exception of sodium acetate, showed a significant increase in the degree of hydration. Sodium acetate formed the lowest amount of magnesium hydroxide, ranging between 1.2 and 12.2% magnesium hydroxide. The largest percentage (56.7%) of magnesium hydroxide was formed from hydration in magnesium acetate.CONCLUSION: It seems that MgO hydration is a dissolution‐precipitation process controlled by the dissolution of MgO. The increased degree of hydration in magnesium acetate is possibly due to the presence of acetate and Mg2+ ions. Copyright © 2010 Society of Chemical Industry
Let's find the evidence: an analogue study of confirmation bias in criminal investigations
Authors: Rassin Eric , Eerland Anita , Kuijpers Ilse 
Published: July 2010

AbstractPeople involved in criminal proceedings (e.g. police officers, district attorneys, judges, and jury members) may run the risk of developing confirmation bias, or tunnel vision. That is, these parties may readily become convinced that the suspect is guilty, and may then no longer be open to alternative scenarios in which the suspect is actually innocent. This may be reflected in a preference for guilt‐confirming investigation endeavours, as opposed to investigations that are aimed at confirming, or even excluding, alternative scenarios. In three studies, participants read a case file, and were subsequently instructed to select additional police investigations. Some of these additional endeavours were guilt‐confirming (i.e. incriminating), whereas others were disconfirming (i.e. exonerating). Results suggest that additional investigation search was guided by an initial assessment of the suspect's guilt (Study 1). Furthermore, participants' tendency to select incriminating investigations increased with increased crime severity, and with the strength of the evidence present in the case file. Finally, the selection of incriminating investigations was associated with conviction rates (Study 3). However, in general, participants did not favour incriminating endeavours. That is, in the three studies, the percentages of selected incriminating endeavours did hardly or not exceed 50%. Copyright © 2010 John Wiley & Sons, Ltd.
Comparison of 3.0 T proton magnetic resonance spectroscopy short and long echo‐time measures of intramyocellular lipids in obese and normal‐weight women
Authors: Bredella Miriam A. , Ghomi Reza Hosseini , Thomas Bijoy J. , Miller Karen K. , Torriani Martin 
Published: July 2010

AbstractPurpose:To compare correlations of intramyocellular lipids (IMCL) measured by short and long echo‐time proton magnetic resonance spectroscopy (1H‐MRS) with indices of body composition and insulin resistance in obese and normal‐weight women.Materials and Methods:We quantified IMCL of tibialis anterior (TA) and soleus (SOL) muscles in 52 premenopausal women (37 obese and 15 normal weight) using single‐voxel 1H‐MRS PRESS at 3.0 T with short (30 msec) and long (144 msec) echo times. Statistical analyses were performed to determine correlations of IMCL with body composition as determined by computed tomography (CT) and insulin resistance indices and to compare correlation coefficients from short and long echo‐time data. Signal‐to‐noise ratio (SNR), linewidth, and coefficients of variation (CV) of short and long echo‐time spectra were calculated.Results:Short and long echo‐time IMCL from TA and SOL significantly correlated with body mass index (BMI) and abdominal fat depots (r = 0.32 to 0.70, P = <0.05), liver density (r = −0.39 to −0.50, P < 0.05), and glucose area under the curve as a measure of insulin resistance (r = 0.47 to 0.49, P < 0.05). There was no significant difference between correlation coefficients of short and long echo‐time spectra (P > 0.5). Short echo‐time IMCL in both muscles showed significantly higher SNR (P < 0.0001) and lower CVs when compared to long echo‐time acquisitions. Linewidth measures were not significantly different between groups.Conclusion:IMCL quantification using short and long echo‐time 1H‐MRS at 3.0 T is useful to detect differences in muscle lipid content in obese and normal‐weight subjects. In addition, IMCL correlates with body composition and markers of insulin resistance in this population with no significant difference in correlations between short and long echo‐times. Short echo‐time IMCL quantification of TA and SOL muscles at 3.0 T was superior to long echo‐time due to better SNR and better reproducibility. J. Magn. Reson. Imaging 2010;32:388–393. © 2010 Wiley‐Liss, Inc.
The influence of the b‐value combination on apparent diffusion coefficient based differentiation between malignant and benign tissue in cervical cancer
Authors: Hoogendam Jacob P. , Klerkx Wenche M. , de Kort Gerard A.P. , Bipat Shandra , Zweemer Ronald P. , Sie‐Go Daisy M.D.S. , Verheijen René H.M. , Mali Willem P.T.M. , Veldhuis Wouter B. 
Published: July 2010

AbstractPurpose:To analyze the influence of different b‐value combinations on apparent diffusion coefficient (ADC)‐based differentiation of known malignant and benign tissue in cervical cancer patients.Materials and Methods:A total of 35 patients with stage IB1, IB2, IIA cervical cancer underwent a 3.0T MRI scan prior to radical hysterectomy and pelvic lymph node dissection. Conventional T1‐ and T2‐weighted sequences and a diffusion‐weighted sequence (b = 0, 150, 500, 1000 seconds/mm2) were performed. Regions‐of‐interest (ROI) were drawn on ADC maps derived from five different b‐value combinations (0, 500; 0, 150, 500; 0, 1000; 0, 150, 500, 1000; 150, 500, 1000 seconds/mm2). The influence of the b‐value combination on ADC‐based differentiation of benign and malignant tissue was analyzed using receiver‐operating‐characteristics curves.Results:For all b‐value combinations, ADCs were significantly lower (P < 0.001) in cervical malignancies (1.15 ± 0.21·10−3; 1.10 ± 0.21·10−3; 0.97 ± 0.18·10−3; 0.97 ± 0.23·10−3 and 0.85 ± 0.18·10−3 mm2/second respectively to the aforementioned b‐value combinations) than in benign cervix (2.08 ± 0.31·10−3; 2.00 ± 0.29·10−3; 1.62 ± 0.23·10−3; 1.54 ± 0.21·10−3 and 1.42 ± 0.22·10−3 mm2/second respectively). The diagnostic accuracy was high for all b‐value combinations and without statistical differences between the combinations.Conclusion:ADC‐based differentiation of benign from malignant cervical tissue is independent of the tested b‐value combinations. The results support the inclusion and possible pooling of studies using different b‐value combinations in meta‐analyses on ADC‐based tissue differentiation in cervical cancer. J. Magn. Reson. Imaging 2010;32:376–382. © 2010 Wiley‐Liss, Inc.
Two‐ and three‐dimensional multinuclear stray‐field imaging of rotating samples with magic‐angle spinning (STRAFI‐MAS): From bio to inorganic materials
Authors: Wong Alan , Sakellariou Dimitris 
Published: July 2010

AbstractPurpose:To revisit and illustrate the potential of a simple and effective multidimensional stray‐field imaging technique with magic‐angle spinning, known as STRAFI‐MAS.Materials and Methods:STRAFI‐MAS images are acquired with a standard NMR magnet and a traditional magic‐angle sample spinning (MAS) probe. The stray‐field gradients are achieved by placing the MAS probe, along the z‐direction, at a distance from the center of the magnet. No pulsed‐field gradients are applied. The multidimensional spatial encoding is carried out by synchronizing the radiofrequency pulses with the sample MAS rotation.Results:Two‐dimensional (2D) and 3D multinuclear images of various phantoms, including a tibia bone and silicon carbide, are recorded. Images of inorganic solids containing quadrupolar nuclei, 23Na and 27Al, are also explored for the first time by STRAFI‐MAS.Conclusion:We have demonstrated that STRAFI‐MAS is a simple and user‐friendly technique for multidimensional imaging without the need of imaging equipment. With the current advancements in NMR and MRI methodologies, STRAFI‐MAS is expected to be further developed and improved. We anticipate that STRAFI‐MAS can spark a wide spectrum of interest, from material to bio science, where can benefit from high‐resolution images. J. Magn. Reson. Imaging 2010;32:418–423. © 2010 Wiley‐Liss, Inc.
Feasibility of small bowel flow rate measurement with MRI
Authors: Gutzeit Andreas , Patak Michael A. , von Weymarn Constantin , Graf Nicole , Doert Aleksis , Willemse Edwin , Binkert Christoph A. , Froehlich Johannes M. 
Published: July 2010

AbstractObjective:To evaluate whether it is feasible to measure the segmental flux of small bowel content using MR phase‐contrast (PC) pulse sequences.Materials and methods:Using a phantom the accuracy of flux measurements was validated. Afterwards, 10 volunteers were included in a prospective clinical trial. To provide standardized small bowel distension, all volunteers ingested four equal portions of 400 mL of water doped with 5 mL gadoterate and 5.8 g Metamucil mite. The MR protocol covering the sagittal cross‐sections of the small bowel included several two‐dimensional (2D) PC sequences with a velocity encoding of 7 cm/second at a temporal resolution of 0.55 second. As proof of concept time‐dependent flux was measured after intravenous (i.v.) administration of a spasmolytic agent in one volunteer.Results:Phantom measurements resulted in an excellent correlation between pump and PC measured flow rates (R = 0.999). Time‐resolved small bowel flux was successfully measured in distended small bowel loops of all volunteers. A mean flow rate of 0.188 mL/second (standard deviation ± 0.144 mL/second) was documented. The flux plots presented a sinus wave‐like shape with regular aboral and oral flow. A spasmolytic effect both on flux and motility could be shown with residual flux despite complete arrest of small bowel motility.Conclusion:PC MRI allows time‐resolved in vivo measurement of small bowel flux in single well‐distended bowel loops filled with gadolinium‐doped aqueous solution. J. Magn. Reson. Imaging 2010;32:345–351. © 2010 Wiley‐Liss, Inc.
Glioma morphology and tumor‐induced vascular alterations revealed in seven rodent glioma models by in vivo magnetic resonance imaging and angiography
Authors: Doblas Sabrina , He Ting , Saunders Debbie , Pearson Jamie , Hoyle Jessica , Smith Nataliya , Lerner Megan , Towner Rheal A. 
Published: July 2010

AbstractPurpose:To evaluate the added value of non–contrast‐enhanced MR angiography (MRA) to conventional MR imaging for a detailed characterization of different rodent glioma models.Materials and Methods:Intracerebral tumor cell implantation and chemical induction methods were implemented to obtain rat C6, 9L/LacZ, F98, RG2, and ethyl‐nitrosourea (ENU) ‐induced glioma models, a human U87 MG tumor model as well as a mouse GL261 glioma model. MR assessments were regularly conducted on a 7 Tesla Bruker BioSpin system. The tumor border sharpness and growth characteristics of each glioma model were assessed from T2‐weighted images. Neovascularization and vascular alterations inherent to each model were characterized by assessing absolute blood volumes, vessel density, length, and diameter using Mathematica and Amira software.Results:The 9L/LacZ and ENU gliomas both presented flaws that hinder their use as reliable brain tumor models. C6 gliomas were slightly invasive and induced moderate vascular alterations, whereas GL261 tumors dramatically altered the brain vessels in the glioma region. F98, RG2, and U87 are infiltrative models that produced dramatic vascular alterations.Conclusion:MRI and MRA provided crucial in vivo information to identify a distinctive “fingerprint” for each of our seven rodent glioma models. J. Magn. Reson. Imaging 2010;32:267–275. © 2010 Wiley‐Liss, Inc.
Interindividual, repositioning, and time‐of‐day effects on single voxel proton MR spectroscopy of the anterior cingulate cortex
Authors: Soreni Noam , Noseworthy Michael D. , Konyer Norman B. , Pullenayegum Eleanor , Schachar Russell 
Published: July 2010

AbstractPurpose:To measure interindividual, repositioning, and time‐of‐day effects of single voxel PRESS (Point RESolved Spectroscopy) proton magnetic resonance spectroscopy (1H‐MRS) acquisition of the anterior cingulate cortex (AC) in healthy human subjects.Materials and Methods:AC 1H‐MRS measurements were performed in 15 healthy adult volunteers using a short echo PRESS sequence (GE Healthcare 3 Tesla, TE/TR = 30/2500 ms, 192 acquisitions, 6 cm3 voxels). For each individual, a total of eight spectra were obtained during two identical scanning sessions separated by 3.5 h. In each, two consecutive AC spectra were acquired. After the first two scans, the subject left the scanner, then immediately returned for repositioning and acquisition of two more consecutive spectra. The subject then left the imaging centre to return 3.5 h later for a repeated procedure. Spectroscopic postprocessing was done using LCmodel. Interindividual, repositioning and time‐of‐day effects were measured using restricted maximum likelihood (REML) models of variance components analysis, where response variables were levels of creatine/phosphocreatine (Cr), N‐acetyl‐aspartate (NAA), myo‐inositol (mI), choline (Cho), and the glutamate‐glutamine complex (Glx).Results:Interindividual effects were markedly higher than time‐of‐day and repositioning effects for all metabolites.Conclusion:Our findings show that 1H‐MRS measurements of the AC are sensitive to interindividual differences, while time of day and repositioning are markedly less important. J. Magn. Reson. Imaging 2010;32:276–282. © 2010 Wiley‐Liss, Inc.
Ultra‐short echo time (UTE) MR imaging of the lung: Comparison between normal and emphysematous lungs in mutant mice
Authors: Takahashi Masaya , Togao Osamu , Obara Makoto , van Cauteren Marc , Ohno Yoshiharu , Doi Shigehiro , Kuro‐O Makoto , Malloy Craig , Hsia Connie C. , Dimitrov Ivan 
Published: July 2010

AbstractPurpose:To investigate the utility of ultra‐short echo time (UTE) sequence as pulmonary MRI to detect non‐uniform disruption of lung architecture that is typical of emphysema.Materials and Methods:MRI of the lungs was conducted with a three‐dimensional UTE sequence in transgenic mice with severe emphysema and their wild‐type littermates in a 3 Tesla clinical MR system. Measurements of the signal intensity (SI) and transverse relaxation time (T2*) of the lung parenchyma were performed with various echo times (TEs) ranging from 100 μs to 2 ms.Results:Much higher SI of the lung parenchyma was observed at an UTE of 100 μs compared with longer TEs. The emphysematous lungs had reduced SIs and T2* than the controls, in particular at end‐expiratory phase. The results suggested that both SI and T2* in lung parenchyma measured with the method represent fractional volume of lung tissue.Conclusion:The UTE imaging provided MR signal from the lung parenchyma. Moreover, the UTE sequence was sensitive to emphysematous changes and may provide a direct assessment of lung parenchyma. UTE imaging has the potential to assist detection of localized pathological destruction of lung tissue architecture in emphysema. J. Magn. Reson. Imaging 2010;32:326–333. © 2010 Wiley‐Liss, Inc.