bluuebunny/crossref_metadata_2025_split huggingface dataset:
Comprehensive analysis of CpG island methylator phenotype (CIMP)‐high, ‐low, and ‐negative colorectal cancers based on protein marker expression and molecular features
Authors: Zlobec Inti , Bihl Michel , Foerster Anja , Rufle Alex , Lugli Alessandro 
Published: February 2011

AbstractCpG island methylator phenotype (CIMP) is being investigated for its role in the molecular and prognostic classification of colorectal cancer patients but is also emerging as a factor with the potential to influence clinical decision‐making. We report a comprehensive analysis of clinico‐pathological and molecular features (KRAS, BRAF and microsatellite instability, MSI) as well as of selected tumour‐ and host‐related protein markers characterizing CIMP‐high (CIMP‐H), ‐low, and ‐negative colorectal cancers. Immunohistochemical analysis for 48 protein markers and molecular analysis of CIMP (CIMP‐H: ≥ 4/5 methylated genes), MSI (MSI‐H: ≥ 2 instable genes), KRAS, and BRAF were performed on 337 colorectal cancers. Simple and multiple regression analysis and receiver operating characteristic (ROC) curve analysis were performed. CIMP‐H was found in 24 cases (7.1%) and linked (p < 0.0001) to more proximal tumour location, BRAF mutation, MSI‐H, MGMT methylation (p = 0.022), advanced pT classification (p = 0.03), mucinous histology (p = 0.069), and less frequent KRAS mutation (p = 0.067) compared to CIMP‐low or ‐negative cases. Of the 48 protein markers, decreased levels of RKIP (p = 0.0056), EphB2 (p = 0.0045), CK20 (p = 0.002), and Cdx2 (p < 0.0001) and increased numbers of CD8+ intra‐epithelial lymphocytes (p < 0.0001) were related to CIMP‐H, independently of MSI status. In addition to the expected clinico‐pathological and molecular associations, CIMP‐H colorectal cancers are characterized by a loss of protein markers associated with differentiation, and metastasis suppression, and have increased CD8+ T‐lymphocytes regardless of MSI status. In particular, Cdx2 loss seems to strongly predict CIMP‐H in both microsatellite‐stable (MSS) and MSI‐H colorectal cancers. Cdx2 is proposed as a surrogate marker for CIMP‐H. Copyright © 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
Malignant fibrous histiocytoma two years after autologous stem cell transplant for Hodgkin lymphoma: Evidence for genomic instability
Authors: Chandrakasan Shanmuganathan , Ye Christine J. , Chitlur Meera , Mohamed Anwar N. , Rabah Raja , Konski Andre , Heng Henry H. Q. , Savaşan Süreyya 
Published: February 2011

AbstractSecondary malignancies (SMs) in Hodgkin lymphoma (HL) are thought to be related to exposure to alkalating agents, topoisomerase II inhibitors and ionizing radiation, and tend to occur a decade after initial therapy. We report a 14 year old autistic male, who developed malignant fibrous histiocytoma (MFH) two years after autologous stem cell transplantation for advanced stage HL. The MFH and post‐surgical reactive tissues exhibited multiple clonal abnormalities. In addition, PHA‐stimulated peripheral blood lymphocytes showed increased frequency of non‐clonal chromosomal aberrations. The potential role of genomic instability in early onset of SM in our patient is discussed. Pediatr Blood Cancer 2011;56:1143–1145. © 2010 Wiley‐Liss, Inc.
Tele‐practice guidelines for the symptom management of children undergoing cancer treatment
Authors: Tsimicalis Argerie , De Courcy Mary Jo , Di Monte Bruna , Armstrong Christine , Bambury Patti , Constantin Julie , Dagelman Barbara , Eves Marilyn , Jansen Patty , Honeyford Lisa , Stregger Denise 
Published: February 2011

AbstractThe provision of tele‐practice symptom management is often without the provision of evidence‐based guidelines. Under the auspices of the Pediatric Oncology Group of Ontario, a nursing task force was established to appraise the evidence and develop guidelines. Promising new efforts to enhance symptom management through tele‐practice are emerging. Seven guidelines and one documentation tool were created from evidence compiled from case reports, clinical examples, and nonexperimental studies. The symptom management guidelines contribute to the paucity of literature and may serve as a useful resource for health professionals providing telephone advice and conducting tele‐practice symptom management assessments. Pediatr Blood Cancer 2011; 57: 541–548. © 2011 Wiley‐Liss, Inc.
Invasive fungal infections in pediatric oncology
Authors: Mor Meirav , Gilad Gil , Kornreich Liora , Fisher Salvador , Yaniv Isaac , Levy Itzhak 
Published: February 2011

AbstractBackgroundData on the epidemiology and outcome of invasive fungal infections in children with cancer are limited. The aim of the study was to delineate the epidemiologic, clinical features, risk factors, and outcome of invasive fungal infections in this population.ProcedureThe medical records of all children with malignancies diagnosed with an invasive fungal infection in 1998–2006 at a tertiary pediatric medical center were reviewed for demographic, clinical, and laboratory data. Invasive fungal infection was diagnosed according to the latest EORTC/MSG criteria.ResultsOf the 1,047 children hospitalized in the hematology/oncology department during the study period, 75 (7.2%) were diagnosed with a proven (n = 16, 21.3%), probable (n = 18, 24%), or possible (n = 41, 54.7%) invasive fungal infection. Fifteen (20%) had candidemia (non‐albicans in 60%), and 60 (80%) had a mold infection (non‐Aspergillus in 55%). Crude mortality was 21.7%. The most common underlying diseases were myeloid leukemia (n = 26, 34.7%) and acute lymphoblastic leukemia (n = 24, 32%). Compared to other malignancies, acute myeloid leukemia was significantly associated with the development of invasive fungal infections. Profound neutropenia and high treatment intensity were present in 89% and 73% of patients with IFI respectively.ConclusionsThe current mortality rates of invasive fungal infection in children with cancer are lower than previously reported in children and adults. However, the proportion of non‐albicans candidemia is increasing, and non‐Aspergillus molds are emerging as important pathogens, which may have important implications for prophylaxis and empiric therapy. Improved prevention, early detection, and advanced treatment strategies are needed to improve the outcome. Pediatr Blood Cancer 2011;56:1092–1097. © 2011 Wiley‐Liss, Inc.
A cross‐sectional study of overweight in pediatric survivors of acute lymphoblastic leukemia (ALL)
Authors: Love Erin , Schneiderman Jane E. , Stephens Derek , Lee Sylvia , Barron Mary , Tsangaris Elena , Urbach Stacey , Staneland Patricia , Greenberg Mark , Nathan Paul C. 
Published: February 2011

AbstractBackgroundSurvivors of childhood acute lymphoblastic leukemia (ALL) are at risk for overweight, predisposing them to long‐term morbidity. We examined the relationship between body mass index (BMI) and demographic and lifestyle factors in a cohort of ALL survivors.ProcedureWe enrolled consecutive ALL survivors who attended the institutional AfterCare Clinic over a 1‐year period. BMI (kg/m2) at diagnosis, end‐therapy, and current time were calculated. Survivors were classified as overweight/obese (overweightgroup; BMI for age ≥85th percentile) or normal/underweight (normalweight group; BMI for age <85th percentile). We assessed the relationship between current BMI and self‐reported current caloric intake, physical activity, and sedentary behavior.Results102/157 (65%) eligible survivors consented to enrollment. Median age was 14.3 years (range 8.4–18.6) and time from end of treatment 7 years (2.5–15.6). The proportion of overweight survivors was 21%, 45%, and 35% at diagnosis, end‐therapy, and current time, respectively. The overweight group reported less calories (Δ324 kcal,P = 0.018), fat (Δ14.5 g,P = 0.02), and carbohydrates (Δ39 g,P = 0.02) than the normal weight group. These differences disappeared after excluding patients classified as under‐reporting their calorie intake according to the Goldberg cut‐off method. There were no differences in sedentary behavior or activity between groups.ConclusionsMany children with ALL gain weight during therapy and fail to return to normal weight after treatment concludes. Subsequent diet, physical activity, and sedentary behavior do not appear to differ between overweight and normal weight survivors. Clinicians should focus on ways to minimize weight gain during therapy rather than waiting for treatment to conclude. Pediatr Blood Cancer 2011; 57: 1204–1209. © 2011 Wiley Periodicals, Inc.
Preclincial testing of Sorafenib and RAD001 in the Nfflox/flox;DhhCre mouse model of plexiform neurofibroma using magnetic resonance imaging
Authors: Wu Jianqiang , Dombi Eva , Jousma Edwin , Scott Dunn R. , Lindquist Diana , Schnell Beverly M. , Kim Mi‐Ok , Kim AeRang , Widemann Brigitte C. , Cripe Timothy P. , Ratner Nancy 
Published: February 2011

AbstractBackgroundNeurofibromatosis type 1 (NF1) is an inherited disease predisposing affected patients to variable numbers of benign neurofibromas. To date there are no effective chemotherapeutic drugs available for this slow growing tumor. Molecularly targeted agents that aim to slow neurofibroma growth are being tested in clinical trials. So preclinical models for testing potential therapies are urgently needed to prioritize drugs for clinical trials of neurofibromas.ProcedureWe used magnetic resonance imaging (MRI) to monitor neurofibroma development in the Nf1flox/flox;DhhCre mouse model of GEM grade I neurofibroma. Based on studies implicating mTOR and Raf signaling in NF1 mutant cells, we tested the therapeutic effect of RAD001 and Sorafenib in this model. Mice were scanned to establish growth rate followed by 8 weeks of drug treatment, then re‐imaged after the last dose of drug treatment. Tumor volumes were determined by volumetric measurement.ResultsWe found that rate of tumor growth varied among mice, as it does in human patients. RAD001 inhibited its predicted target pS6K, yet there was no significant decrease in the tumor volume in RAD001 treated mice compared to the vehicle control group. Sorafenib inhibited cyclinD1 expression and cell proliferation in tumors, and volumetric measurements identified significant decreases in tumor volume in some mice.ConclusionThe data demonstrate that volumetric MRI analysis can be used to monitor the therapeutic effect in the preclinical neurofibroma drug screening, and suggest that Sorafenib might have clinical activity in some neurofibromas. Pediatr Blood Cancer 2012; 58: 173–180. © 2011 Wiley Periodicals, Inc.
Five‐year single‐center study of asparaginase therapy within the ALL‐BFM 2000 trial
Authors: Schrey Dominik , Speitel Katharina , Lanvers‐Kaminsky Claudia , Gerss Joachim , Möricke Anja , Boos Joachim 
Published: February 2011

AbstractBackgroundTherapeutic drug monitoring (TDM) of asparaginase (ASNase), a fundamental element of acute lymphoblastic leukemia treatment, was integrated in the ALL‐BFM 2000 protocol on a voluntary basis.MethodsOver a 5‐year period, 127 patients (1,355 samples) were monitored for asparaginase activity in a single‐center setting. We report monitoring data from throughout the ASNase containing treatment elements. Additional information obtained on risk stratification, minimal residual disease (MRD), steroid randomization and relapse is discussed in relation to ASNase activity.ResultsAt completion of the induction phase 93% (115/124) of patients showed sufficient ASNase activity (5,000 U/m2 Escherichia coli ASNase), 77 of 86 (90%) monitored patients finished the first re‐intensification element without requiring Erwinia ASNase. MRD, risk stratification and steroid randomization were not associated with significant differences in ASNase activity. Of patients who relapsed, only 25% (3/12) were able to maintain sufficient ASNase activity after E. coli ASNase.ConclusionThis single‐center data set gives a true and unbiased insight into clinical reality of ASNase therapy. It shows no significant relationship between MRD positivity or risk stratification and ASNase treatment intensity. Overall, within the ALL‐BFM 2000 trial, 90% of patients completed first re‐intensification without requiring third‐line Erwinia ASNase. Pediatr Blood Cancer 2011; 57: 378–384. © 2011 Wiley‐Liss, Inc.
Evaluation of the degree of dispersion of nanofillers by mechanical, rheological, and permeability analysis
Authors: Corcione Carola Esposito , Cavallo Anna , Pesce Emanuela , Greco Antonio , Maffezzoli Alfonso 
Published: February 2011

AbstractThe aim of this paper is a comparison of the effectiveness of different macrocharacterization techniques for the prediction of the degree of dispersion and intercalation of bidimensional nanofillers in an amorphous thermoplastic matrix. Organically modified montmorillonites (omMMT) were used as bidimensional nanofillers, whereas amorphous polyethylene‐terephthalate copolymer (PETg) was used as matrix. Wide angle x‐ray diffraction analysis showed no relevant difference between the samples processed at different temperatures, all characterized by a predominantly intercalated structure. On the other hand, transmission electron microscopy (TEM) analysis showed the presence of some degree of exfoliation, as well as the presence of lamellar stacks of different thickness. The aspect ratio of lamellar stacks was estimated by means of rheological, mechanical, and gas permeability analysis. All techniques provided values which are in quite good agreement with TEM analysis. Furthermore, all techniques were able to capture the increase in the lamellar stack aspect ratio with decreasing processing temperature. POLYM. ENG. SCI., 2011. © 2011 Society of Plastics Engineers
Amphiphilic Copolymer Coatings via Plasma Polymerisation Process: Switching and Anti‐Biofouling Characteristics
Authors: Kumar Virendra , Pulpytel Jerome , Giudetti Guido , Rauscher Hubert , Rossi Francois , Arefi‐Khonsari Farzaneh 
Published: February 2011

AbstractEnvironmentally benign‐solvent free plasma process is employed to produce nanostructured PFDA‐co‐DEGDME amphiphilic coatings via plasma co‐polymerisation of 1H,1H,2H,2H‐perfluorodecyl acrylate (PFDA) and diethyleneglycol dimethyl ether (DEGDME) precursors in a low pressure‐RF‐inductively excited tubular plasma reactor using argon as a carrier gas. The plasma‐polymerised coatings are characterised by Fourier transform infrared spectroscopy (FT‐IR), X‐ray photoelectron spectroscopy (XPS), field emission scanning electron microscopy (FESEM), atomic force microscopy (AFM) and contact angle measurements. PFDA‐co‐DEGDME coatings with varying chemical environments and morphologies are achieved by varying the plasma parameters such as, continuous wave (CW) and pulse modulated (PM) plasma mode, and plasma deposition time. Plasma polymerised PFDA‐co‐DEGDME coatings are found to exhibit a switching property in terms of wettability, i.e., from hydrophobic to hydrophilic and vice versa, in response to the contacting environment. Quartz crystal microbalance (QCM) is used to study the adhesion of two model proteins, namely, human serum albumin (HSA) and fibrinogen (FGN) in continuous flow conditions, which reveals the protein repellent, i.e., anti‐biofouling characteristics of the PFDA‐co‐DEGDME amphiphilic coatings. magnified image
The effect of early Pseudomonas aeruginosa treatment on lung function in pediatric cystic fibrosis
Authors: Amin Reshma , Lam Michelle , Dupuis Annie , Ratjen Felix 
Published: February 2011

AbstractObjectiveOur aim was to assess the effect of treatment of early infection of P. aeruginosa on pulmonary function in pediatric CF patients.MethodsThis was a retrospective cohort study of P. aeruginosa negative CF patients followed at Sick Kids from 1990 to 2007. Early P. aeruginosa infection was defined as the first respiratory culture for P. aeruginosa; patients were included if 5 years of follow‐up pulmonary function data were available. Patients were divided into three groups (group 1: never infected, group 2: infected with subsequent clearance, and group 3: chronic infection or still receiving antipseudomonal antibiotics). Hierarchical linear models were used to estimate the effect of P. aeruginosa infection on spirometry. FEV1% predicted was the primary outcome.Results116 patients were included. Forty‐six (40%) patients remained P. aeruginosa negative throughout the observation period, 29 (25%) patients transiently infected with P. aeruginosa, and 41 (35%) patients were either currently infected or still receiving treatment. Baseline lung function was the same for all groups. Annual decline in FEV1% predicted during the study period was not different (−0.6%/year for patients that were never infected and −1.3%/year among patients previously infected).ConclusionsLung function was not different between patients with early P. aeruginosa infection and those that never had P. aeruginosa infection. However given the slow rate of FEV1 decline in the study population, a longer observation period and/or more sensitive outcomes measures may be required to exclude long‐term detrimental effects of transient P. aeruginosa infection on lung function in CF patients. Pediatr. Pulmonol. 2011; 46:554–558. © 2011 Wiley‐Liss, Inc.
Comparison of mechanical ventilatory constraints between continuous and intermittent exercises in healthy prepubescent children
Authors: Borel Benoit , Leclair Erwan , Thevenet Delphine , Beghin Laurent , Gottrand Frédéric , Fabre Claudine 
Published: February 2011

AbstractBackgroundThe aim of this study was to evaluate the occurrence and severity of mechanical ventilatory constraints in healthy prepubescent children during continuous and intermittent exercise.MethodsTwelve prepubescent children (7–11 years old) performed 7 exercises on a treadmill: one graded test for the determination of maximal aerobic speed (MAS), three continuous exercises (CE) at 60, 70, and 80% of MAS and three intermittent exercises (IE), alternating 15 sec of exercise with 15 sec of passive recovery, at 90, 100, and 110% of MAS. During each CE and IE, tidal flow/volume loops were plotted within a maximal flow/volume loop (MFVL) measured at rest before each exercise. Expiratory flow limitation (expFL expressed in %Vt) was defined as the part of exercise tidal volume (Vt) meeting the boundary of MFVL. Breathing strategy was estimated by measuring inspiratory capacity relative to forced vital capacity and tidal volume relative to inspiratory capacity. Other breathing pattern parameters (ventilation VE, Vt, respiratory frequency f) were continuously recorded during exercise.ResultsAn “intensity” effect was found for ${\dot {\rm {V}}E}$ during CE (P < 0.001) but not during IE (P = 0.08). The increase in ${\dot {\rm {V}}E}$ was predominantly assumed by an increase in f for both exercise modalities. During each exercise, several children heterogeneously experienced expFL ranging between 10 and 90%Vt. For all exercises, Vt was predominantly regulated by an increase in Vt/IC with no change in IC/FVC from rest to exercise. Finally, no significant “modality” effect was found for mechanical ventilatory constraint parameters (expFL, Vt/IC, and IC/FVC).DiscussionWe could conclude that neither of the modalities studied induced more mechanical ventilatory constraints than the other, but that exercise intensities specific to each modality might be greater sources of exacerbation for mechanical ventilatory constraints. Pediatr. Pulmonol. 2011; 46:785–794. © 2011 Wiley‐Liss, Inc.
Polymorphisms in GSDMA and GSDMB are associated with asthma susceptibility, atopy and BHR
Authors: Yu Jinho , Kang Mi‐Jin , Kim Byoung‐Ju , Kwon Ji‐Won , Song Young‐Hwa , Choi Won‐Ah , Shin Yee‐Jin , Hong Soo‐Jong 
Published: February 2011

AbstractThe gasdermin A (GSDMA) and gasdermin B (GSDMB) genes are located at 17q21.2. The GSDM family genes have been studied in the gastrointestinal tract but recent reports suggest that GSDMB is associated with childhood asthma in several populations. We investigated the association of the GSDMA and GSDMB variants with asthma in Korean children, and to assess the effect of these variants on intermediate phenotypes of asthma. Asthmatic (n = 778) and normal (n = 522) children were enrolled and genotypes were determined using PCR‐RFLP. Asthma susceptibility was associated with GG of the GSDMA (rs7212938) and TT of GSDMB (rs7216389). And a combination of risk alleles of two polymorphisms was associated with asthma susceptibility and a frequency of those was higher in asthmatic children with increased levels of total IgE (aOR 1.77, 95% CI 1.15–2.72) and BHR (aOR 1.54, 95% CI 0.99–2.40) compared to normal. Also, we observed a significant association between haplotype of two polymorphisms and asthma susceptibility. The region including the GSDMA and GSDMB polymorphisms may be associated with asthma susceptibility and intermediate phenotypes of asthma, such as elevated IgE and BHR, in Korean children with asthma. These results strongly support an important role for the GSDMA and GSDMB in the development of childhood asthma. Pediatr. Pulmonol. 2011; 46:701–708. © 2011 Wiley‐Liss, Inc.
Response of a red clone of Myzus persicae (Hemiptera: Aphididae) to sublethal concentrations of imidacloprid in the laboratory and greenhouse
Authors: Janmaat Alida , Borrow Erin , Matteoni Jim , Jones Gary 
Published: February 2011

AbstractBACKGROUND: Pest resurgence following a pesticide application may occur owing to a stimulatory (hormetic) response to sublethal insecticide concentrations. The objective of the present study was to examine the potential for a greenhouse‐derived red clone of Myzus persicae to exhibit resurgence owing to a hormetic response following a systemic imidacloprid treatment in a bell pepper greenhouse.RESULTS: No differences in mortality and fecundity were observed among apterous adults exposed to sublethal imidacloprid concentrations on excised pepper leaves fed aqueous solutions of imidacloprid. Survival of first‐generation progeny was negatively affected by imidacloprid exposure, yet surviving progeny exhibited no differences in development rates or fecundity from progeny of adults unexposed to imidacloprid. Aphid mortality declined most rapidly in clip cages on pepper leaves at the top of the pepper canopy as compared with leaves present at the middle or bottom of the pepper canopy.CONCLUSION: Imidacloprid decays rapidly in mature pepper plants, resulting in sublethal concentrations in the upper canopy in as little as 4 weeks. Sublethal insecticide concentrations have been implicated in the resurgence of pest populations; however, exposure to sublethal doses of imidacloprid are unlikely to result in pesticide‐induced resurgence of the M. persicae aphid clone examined in this study. Copyright © 2011 Society of Chemical Industry
Ultrafast transient gain in Ge/SiGe quantum wells
Authors: Köster N. S. , Lange C. , Kolata K. , Chatterjee S. , Chrastina D. , Isella G. , von Känel H. , Sigg H. , Schäfer M. , Kira M. , Koch S.W. 
Published: February 2011

AbstractThe ultrafast carrier dynamics of Ge/SiGe quantum wells on Si substrate is investigated by pump‐probe spectroscopy. Spectrally tunable 80 fs pulses emitted by an opto‐parametric amplifier are used to excite the sample and a white‐light supercontinuum generated from a 1 kHz Ti:sapphire regenerative amplifier system is used to probe the sample transmission.Pronounced nonequilibrium effects are observed in the relaxation dynamics. The pump energy dependence of these effects is discussed. If the sample is excited under close‐to‐resonant pumping conditions, transient gain is observed. The findings are explained using a microscopic many‐body theory. (© 2011 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)