bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identifying the default‐mode component in spatial IC analyses of patients with disorders of consciousness
Authors: Soddu Andrea , Vanhaudenhuyse Audrey , Bahri Mohamed Ali , Bruno Marie‐Aurelie , Boly Mélanie , Demertzi Athena , Tshibanda Jean‐Flory , Phillips Christophe , Stanziano Mario , Ovadia‐Caro Smadar , Nir Yuval , Maquet Pierre , Papa Michele , Malach Rafael , Laureys Steven , Noirhomme Quentin 
Published: April 2011

AbstractObjectives:Recent fMRI studies have shown that it is possible to reliably identify the default‐mode network (DMN) in the absence of any task, by resting‐state connectivity analyses in healthy volunteers. We here aimed to identify the DMN in the challenging patient population of disorders of consciousness encountered following coma. Experimental design: A spatial independent component analysis‐based methodology permitted DMN assessment, decomposing connectivity in all its different sources either neuronal or artifactual. Three different selection criteria were introduced assessing anticorrelation‐corrected connectivity with or without an automatic masking procedure and calculating connectivity scores encompassing both spatial and temporal properties. These three methods were validated on 10 healthy controls and applied to an independent group of 8 healthy controls and 11 severely brain‐damaged patients [locked‐in syndrome (n = 2), minimally conscious (n = 1), and vegetative state (n = 8)]. Principal observations: All vegetative patients showed fewer connections in the default‐mode areas, when compared with controls, contrary to locked‐in patients who showed near‐normal connectivity. In the minimally conscious‐state patient, only the two selection criteria considering both spatial and temporal properties were able to identify an intact right lateralized BOLD connectivity pattern, and metabolic PET data suggested its neuronal origin. Conclusions: When assessing resting‐state connectivity in patients with disorders of consciousness, it is important to use a methodology excluding non‐neuronal contributions caused by head motion, respiration, and heart rate artifacts encountered in all studied patients. Hum Brain Mapp, 2012. © 2011 Wiley Periodicals, Inc.
Hh‐Gli signaling pathway activity in oral and oropharyngeal squamous cell carcinoma
Authors: Leovic Dinko , Sabol Maja , Ozretic Petar , Musani Vesna , Car Diana , Marjanovic Ksenija , Zubcic Vedran , Sabol Ivan , Sikora Miroslav , Grce Magdalena , Glavas‐Obrovac Ljubica , Levanat Sonja 
Published: April 2011

AbstractBackgroundThe aim of this study was to examine the role of Hh‐Gli signaling in oral and oropharyngeal squamous cell carcinoma (SCC). The role of this signaling pathway in SCC formation has not yet been elucidated.MethodsSixty‐four tissue and blood samples were collected from 60 patients with SCC, all tobacco and alcohol users. An additional six buccal mucosa tissue samples were collected from nonsmokers and nondrinkers as control tissue.ResultsHedgehog‐Gli pathway components were associated with clinical and pathologic features. Broders' grade and N stage were associated with higher Ptch1 and lower Gli1 expression. Tumor stage was negatively associated with Smo expression, and tumor size was positively associated with p16 expression. Ptch1 and Shh were frequently detected in the surrounding stroma. Ptch1 was found to be correlated with p16 expression, as well as with survivin expression.ConclusionsThe signaling pathway is activated in SCC and inducible in vitro by Shh protein. © 2011 Wiley Periodicals, Inc. Head Neck, 2012
New Anthraquinone and Iridoid Glycosides from the Stems of Hedyotis hedyotidea
Authors: Hu Xiao‐Ping , Zhang Shu‐Wei , Liu Shan‐Shan , Xuan Li‐Jiang 
Published: April 2011

AbstractFive new anthraquinone glycosides, hedanthrosides A–E (1–5, resp.) and two new iridoid glycosides, hediridosides A and B (6 and 7, resp.), along with two known anthraquinones and four known iridoids, were isolated from the stems of Hedyotis hedyotidea (DC.) Merr. The structures of the new compounds were elucidated on the basis of 1D‐ and 2D‐NMR, and HR‐MS analysis and chemical methods.
Two Glycosides and Other Constituents from Anemone tomentosa Roots
Authors: Hu Hao‐Bin , Zheng Xu‐Dong , Zhu Ji‐Hua , Zhang Xiao‐Wei 
Published: April 2011

AbstractTwo new natural products, i.e., 1‐O,2‐O‐[(1S,2S)‐1‐(3‐acetyl‐2,4,6‐trihydroxyphenyl)‐3‐hydroxypropane‐1,3‐diyl]‐β‐D‐glucopyranose (=tomentoside I; 1) and (3β)‐3‐(acetyloxy)ferruginol 12‐[6‐O‐(4‐hydroxy‐3,5‐dimethoxybenzoyl)‐β‐D‐glucopyranoside] (=tomentoside II; 2), were isolated from the EtOH extract of the root of Anemone tomentosa, together with five known compounds. Their structures were characterized by means of spectroscopic methods, especially by 1H‐, 13C‐, and 2D‐NMR and HR‐MS, as well as by chemical methods and comparison with literature data.
Silica Nanoparticles as a Highly Efficient Catalyst for the One‐Pot Synthesis of 2‐Hydroxyacetamide Derivatives from Isocyanides and Electron‐Poor Aromatic Aldehydes
Authors: Ramazani Ali , Mahyari Amir , Lashgari Hamed , Ślepokura Katarzyna , Lis Tadeusz 
Published: April 2011

AbstractReaction of an isocyanide with an electron‐poor aromatic aldehyde in the presence of silica nanoparticles (silica NP; ca. 42 nm) proceeds smoothly at room temperature to afford 2‐hydroxyacetamide derivatives in high yields (Scheme 1 and Table 1).
Three New Triterpenoid Saponins from Ardisia crenata
Authors: Liu Dai‐Lin , Wang Nai‐Li , Zhang Xue , Yao Xin‐Sheng 
Published: April 2011

AbstractThree new triterpenoid saponins, ardisicrenoside I (1), ardisicrenoside J (2), and ardisicrenoside M (3), along with eight known compounds, were isolated from the roots of Ardisia crenata Sims. Their structures were elucidated as 16α‐hydroxy‐30,30‐dimethoxy‐3β‐O‐{β‐D‐xylopyranosyl‐(1→2)‐β‐D‐glucopyranosyl‐(1→4)‐[β‐D‐glucopyranosyl‐(1→2)]‐α‐L‐arabinopyranosyl}‐13β,28‐epoxyoleanane (1), 16α‐hydroxy‐30,30‐dimethoxy‐3β‐O‐{α‐L‐rhamnopyranosyl‐(1→2)‐β‐D‐glucopyranosyl‐(1→4)‐[β‐D‐glucopyranosyl‐(1→2)]‐α‐L‐arabinopyranosyl}‐13β,28‐epoxyoleanane (2), 30,30‐dimethoxy‐16‐oxo‐3β‐O‐{β‐D‐xylopyranosyl‐(1→2)‐β‐D‐glucopyranosyl‐(1→4)‐[β‐D‐glucopyranosyl‐(1→2)]‐α‐L‐arabinopyranosyl}‐13β,28‐epoxyoleanane (3), ardisiacrispin A (4), ardisiacrispin B (5), ardisicrenoside B (6), ardisicrenoside A (7), ardisicrenoside H (8), ardisicrenoside G (9), cyclamiretin A‐3β‐O‐β‐D‐xylopyranosyl‐(1→2)‐β‐D‐glucopyranosyl‐(1→4)‐α‐L‐arabinopyranoside (10), and cyclamiretin A‐3β‐O‐α‐L‐rhamnopyranosyl‐(1→2)‐β‐D‐glucopyranosyl‐(1→4)‐α‐L‐arabinopyranoside (11) by means of chemical and spectral analysis, and their cytotoxicities were evaluated in vitro.
Influence of Variation of a Side Chain on the Folding Equilibrium of a β‐Peptide
Authors: Lin Zhixiong , Hodel Florian H. , van Gunsteren Wilfred F. 
Published: April 2011

AbstractThe ability to design well‐folding β‐peptides with a specific biological activity requires detailed insight into the relationship between the β‐amino acid sequence and the three‐dimensional structure of the peptide. Here, we present a molecular‐dynamics (MD) study of the influence of a variation of a side chain on the folding equilibrium of a β‐heptapeptide that folds into a 314‐helical structure. The side chain of the 5th residue, a valine, was changed into five differently branched side chains of different lengths and polarity, Ala, Leu, Ile, Ser, and Thr. Two computational techniques, long‐time MD simulations and the one‐step perturbation method, were used to obtain free enthalpies of folding. The simulations show that all six peptides exhibit similar folding behavior, and that their dominant fold is the same, i.e., a 314‐helix. Despite the similarities of their structural properties, a small stabilization effect of ca. 2 kJ mol−1 on the folding equilibrium of the 314‐helical structure due to a branching Cγ‐atom in the β3‐side chain is observed. These results confirm those of previous circular dichroism (CD) studies. The length of side chain and its polarity seem to have no apparent (de)stabilization effect. Application of the cost‐effective one‐step perturbation method to predict free‐enthalpy differences appeared to yield an overall accuracy of about kBT, which is not sufficient to detect the small stabilization effect.
Factors controlling spatial and seasonal distributions of precipitation δ18O in China
Authors: Zhao Liangju , Xiao Honglang , Zhou Maoxian , Cheng Guodong , Wang Lixin , Yin Li , Ren Juan 
Published: April 2011

AbstractUtilising datasets from the Global Network of Isotopes in Precipitation of the International Atomic Energy Agency, and previous isotopic studies, we investigated δ18O spatial and temporal patterns in Chinese precipitation. Significantly positive relationships existed between precipitation δ18O and air temperature above the north of 35°N and in high altitude regions above 32°N. Significantly negative relationships between precipitation δ18O and the precipitation amount existed below south of 35°N. These temperature and precipitation effects became stronger with increasing altitude except in high altitude regions between 32°N and 35°N. The NCEP/NCAR reanalysis data from 1980 to 2004 showed that variations in spatial and seasonal wind fields at 700 hpa and total precipitable water from the ground to the top of the atmosphere were correlated with the monthly spatial distribution of precipitation δ18O. Basing on this relationship, we established quantitative correlations between the mean monthly precipitation δ18O and both latitude and temperature in different seasons. We found that spatial variations in precipitation δ18O could be described well using the Bowen–Wilkinson model and second‐order equations developed during the present study only in winter (from December to February). During the rest of the year, patterns were too complex to predict using simple models. The results suggest that it is difficult to demonstrate variations of precipitation δ18O throughout the year and for all regions of China using a single model. Moreover, the new models for the relationships among precipitation, latitude, and temperature were better able to depict the variations in precipitation δ18O than the Bowen–Wilkinson model. Copyright © 2011 John Wiley & Sons, Ltd.
Adult attachment style and stress as risk factors for early maternal sensitivity and negativity
Authors: Mills‐Koonce W. Roger , Appleyard Karen , Barnett Melissa , Deng Min , Putallaz Martha , Cox Martha 
Published: April 2011

AbstractThe current study examined the individual and joint effects of self‐reported adult attachment style, psychological distress, and parenting stress on maternal caregiving behaviors at 6 and 12 months of child age. We proposed a diathesis‐stress model to examine the potential deleterious effects of stress for mothers with insecure adult attachment styles. Data from 137 mothers were gathered by the longitudinal Durham Child Health and Development Study. Mothers provided self‐reports using C. Hazan and P. Shaver's (1987) Adult Attachment Style measure, the Brief Symptom Inventory (L.R. Derogatis & P.M. Spencer, 1982), and the Parent Stress Inventory (R.R. Abidin, 1995); observations of parenting data were made from 10‐min free‐play interactions. Consistently avoidant mothers were less sensitive with their infants than were consistently secure mothers; however, this effect was limited to avoidant mothers who experienced elevated levels of psychological distress. Results suggest that the association between insecure adult attachment style and insensitive parenting behavior is moderated by concurrent psychosocial stress. Clinical implications for these findings are discussed.
Deconstructing antenatal depression: What is it that matters for neonatal behavioral functioning?
Authors: Goodman Sherryl H. , Rouse Matthew H. , Long Qi , Ji Shuang , Brand Sarah R. 
Published: April 2011

AbstractThis prospective study examined the variability within clinical characteristics of antenatal maternal depression and cortisol levels for associations with newborn infant behavior using the Neonatal Behavioral Assessment Scale (NBAS; T.B. Brazelton, 1984). Participants were 81 pregnant women at risk for perinatal depression given their histories of depression prior to pregnancy. We took into consideration not only whether the woman experienced antenatal depression but also whether the depression met diagnostic criteria and variability in timing (onset and occurrence) of antenatal depression and symptom severity. Infants of mothers who became depressed during pregnancy scored less optimally on a subset of the NBAS scales, specifically those scales related to infant neuroregulation. Among the clinical characteristics of depression, the fetus' overall exposure to mothers' depression (reflected in the mean) was most often and most strongly associated with NBAS scales. In terms of timing, third‐trimester exposure was significantly related to newborn behavior. The findings are discussed within the S.H. Goodman and I.H. Gotlib (1999) model for transmission of psychopathology to offspring of depressed mothers.
Which preventive interventions effectively enhance depressed mothers' sensitivity? A meta‐analysis
Authors: Kersten‐Alvarez Laura E. , Hosman Clemens M.H. , Riksen‐Walraven J. Marianne , Van Doesum Karin T.M. , Hoefnagels Cees 
Published: April 2011

AbstractImproving depressed mothers' sensitivity is assumed to be a key element in preventing adverse outcomes for children of such mothers. This meta‐analysis examines the short‐term effectiveness of preventive interventions in terms of enhancing depressed mothers' sensitivity toward their child and investigates what type of intervention is most effective. Thirteen interventions, reported in 10 controlled outcome studies, met the inclusion criteria (N = 918). Meta‐analytic results showed a small to medium, significant mean effect size (g = 0.32) with large variation in individual effect sizes (−0.56–1.76). Interventions including baby massage were highly effective in improving maternal sensitivity (g = 0.85). In contrast, individual therapy for the mother proved ineffective in terms of improving maternal sensitivity (g = −0.00). Two other significant predictors of greater effect sizes were the inclusion of a support group and the use of a higher number of intervention methods; however, the significance of these results was largely accounted for by one single study. Our meta‐analysis confirms that depressed mothers' sensitivity can be improved by preventive intervention and suggests that baby massage may be an effective intervention method to evoke short‐term changes in maternal sensitivity. It is unclear whether these changes are maintained over time.
A randomized controlled trial of mother–infant psychoanalytic treatment: II. Predictive and moderating influences of qualitative patient factors
Authors: Salomonsson Björn , Sandell Rolf 
Published: April 2011

AbstractA randomized control trial was performed on 75 dyads in Stockholm, Sweden, with infants under 1½ years. It recruited mothers who worried about the babies, themselves as mothers, and/or the mother–baby relationship. Two groups of mother–infant dyads were compared. One received only Child Health Centre care (the “CHCC” group) while the other received mother–infant psychoanalytic treatment plus CHCC (the “MIP” group). Significant treatment effects were found on mother‐reported depression, interviewer‐rated dyadic relationship qualities and externally rated maternal sensitivity, and near‐significant effects on mother‐reported stress, all in favor of MIP. The objective of this study is to investigate the predictive and moderating influences on outcomes by qualitatively assessed maternal and infant characteristics. The qualitative factors covered maternal suitability for psychoanalysis, and “ideal types” of mother and child, respectively. Outcome measures from two interviews with a 6‐month interval were depression (Edinburgh Postnatal Depression Scale (J. Cox, J. Holden, & R. Sagovsky, 1987), stress (Swedish Parental Stress Questionnaire (M. Östberg, B. Hagekull, & S. Wettergren, 1997), distress (Swedish Symptom Checklist‐90 (SCL‐90; L.R. Derogatis, 1994; M. Fridell, Z. Cesarec, M. Johansson, & S. Malling Thorsen, 2002) and infant social and emotional functioning (Ages and Stages Questionnaire: Social–Emotional (J. Squires, D. Bricker, K. Heo, & E. Twombly, 2002), relationship qualities (Parent–Infant Global Assessment Scale (PIR‐GAS; ZERO TO THREE, 2005), and videotaped interactions (Emotional Availability Scales, Z. Biringen, J.L. Robinson, & R.N. Emde, 1998). Suitability for psychoanalysis predicted outcome only on the PIR‐GAS. Two overarching maternal ideal types were created, reflecting their attitude to the psychoanalytic process: “Participators” and “Abandoned.” The Participators benefited more from MIP than they did from CHCC on maternal interactive sensitivity. A contrasting, but nonsignificant, pattern was found among the Abandoned mothers. Two ideal types of babies emerged: those “Affected” and “Unaffected” by the disturbance, respectively. Among Affected babies, dyadic relationships and sensitivity among their mothers improved significantly more from MIP than they did from CHCC. The superior effects of MIP applied especially to Participator mothers and Affected infants. For Abandoned mothers and Unaffected infants, CHCC seemed to be of equal value.
Protein coadaptation and the design of novel approaches to identify protein–protein interactions
Authors: Fares Mario A. , Ruiz‐González Mario X. , Labrador Juan Pablo 
Published: April 2011

AbstractProteins rarely function in isolation but they form part of complex networks of interactions with other proteins within or among cells. The importance of a particular protein for cell viability is directly dependent upon the number of interactions where it participates and the function it performs: the larger the number of interactions of a protein the greater its functional importance is for the cell. With the advent of genome sequencing and “omics” technologies it became feasible conducting large‐scale searches for protein interacting partners. Unfortunately, the accuracy of such analyses has been underwhelming owing to methodological limitations and to the inherent complexity of protein interactions. In addition to these experimental approaches, many computational methods have been developed to identify protein–protein interactions by assuming that interacting proteins coevolve resulting from the coadaptation dynamics between the amino acids of their interacting faces. We review the main technological advances made in the field of interactomics and discuss the feasibility of computational methods to identify protein–protein interactions based on the estimation of coevolution. As proof‐of‐concept, we present a classical case study: the interactions of cell surface proteins (receptors) and their ligands. Finally, we take this discussion one step forward to include interactions between organisms and species to understand the generation of biological complexity. Development of technologies for accurate detection of protein–protein interactions may shed light on processes that go from the fine‐tuning of pathways and metabolic networks to the emergence of biological complexity. © 2011 IUBMB IUBMB Life, 63(4): 264–271, 2011
Human dental pulp progenitor cell behavior on aqueous and hexafluoroisopropanol based silk scaffolds
Authors: Zhang Weibo , Ahluwalia Ivy Pruitt , Literman Robert , Kaplan David L. , Yelick Pamela C. 
Published: April 2011

AbstractSilk scaffolds have been successfully used for a variety of tissue engineering applications due to their biocompatibility, diverse physical characteristics, and ability to support cell attachment and proliferation. Our prior characterization of 4‐day postnatal rat tooth bud cells grown on hexafluoro‐2‐propanol (HFIP) silk scaffolds showed that the silk scaffolds not only supported osteodentin formation, but also guided the size and shape of the formed osteodentin. In this study, interactions between human dental pulp cells and HFIP and aqueous based silk scaffolds were studied under both in vitro and in vivo conditions. Silk scaffold porosity and incorporation of RGD and DMP peptides were examined. We found that the degradation of aqueous based silk is much faster than HFIP based silk scaffolds. Also, HFIP based silk scaffolds supported the soft dental pulp formation better than the aqueous based silk scaffolds. No distinct hard tissue regeneration was found in any of the implants, with or without additional cells. We conclude that alternative silk scaffold materials, and hDSC pre‐seeding cell treatments or sorting and enrichment methods, need to be considered for successful dental hard tissue regeneration. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2011.
Hydrophilized 3D porous scaffold for effective plasmid DNA delivery
Authors: Oh Se Heang , Kim Tae Ho , Jang Sung Hwan , Im Gun Il , Lee Jin Ho 
Published: April 2011

AbstractIn this study, hydrophilic PLGA/Pluronic F127 scaffolds loaded with a pDNA/PEI‐PEG complex were prepared to estimate their potential use as a polymeric matrix for pDNA delivery. The scaffold was fabricated by a novel precipitation/particulate leaching method. The prepared pDNA/PEI‐PEG complex‐loaded PLGA/Pluronic F127 scaffold exhibited a highly porous (porosity, 93–95%) and open pore structure, as well as hydrophilicity, which can provide the good environment for cell adhesion and growth. The pDNA/PEI‐PEG complexes were efficiently loaded into the PLGA/Pluronic F127 scaffold and continuously released from the scaffolds up to ∼90% of the initial loading amount over a period of 8 wk, which may lead to continuous gene transfection into human bone marrow mesenchymal stem cells (hBMMSCs). From the in vitro cell culture in the scaffolds for transfection, it was observed that the pDNA/PEI‐PEG complex‐loaded hydrophilic PLGA/Pluronic F127 scaffold has a higher transfection efficiency of the pDNA/PEI‐PEG complexes into hBMMSCs than the hydrophobic PLGA ones. The cell viability associated with the pDNA/PEI‐PEG complexes released from the PLGA/Pluronic F127 scaffold was not significantly different from that of the PLGA/Pluronic F127 scaffold without pDNA, indicating its low cytotoxicity, probably due to the sustained release of the pDNA/PEI‐PEG complex from the scaffolds. From these results, we could suggest that the pDNA/PEI‐PEG complex‐loaded hydrophilic PLGA/Pluronic F127 scaffold can be an effective gene delivery system for 3D tissue formation. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2011.
Controlling neurite outgrowth with patterned substrates
Authors: Yang In Hong , Co Carlos C. , Ho Chia‐Chi 
Published: April 2011

AbstractIn vivo, neurons form neurites, one of which develops into the axon while others become dendrites. While this neuritogenesis process is well programmed in vivo, there are limited methods to control the number and location of neurite extension in vitro. Here we report a method to control neuritogenesis by confining neurons in specific regions using cell resistant poly(oligoethyleneglycol methacrylate‐co‐methacrylic acid (OEGMA‐co‐MA)) or poly(ethyleneglycol‐block‐lactic acid) PEG‐PLA. Line patterned substrates reduce multiple extension of neurites and stimulate bi‐directional neurite budding for PC12 and cortical neurons. PC12 cells on 20 and 30 μm line patterns extended one neurite in each direction along the line pattern while cortical neuron on 20 and 30 μm line patterns extended one or two neurites in each direction along the line pattern. Statistical analysis of neurite lengths revealed that PC12 cells and cortical neurons on line patterns extend longer neurites. The ability to guide formation of neurites on patterned substrates is useful for generating neural networks and promoting neurite elongation. © 2011 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 2011.