bluuebunny/crossref_metadata_2025_split huggingface dataset:
Easy-to-set-up image analysis characterizes phenotypic diversity in the growth of mushroom-forming fungusSchizophyllum commune
Authors: Matsumae Hiromi , Sudo Megumi , Imanishi Tadashi , Hosoya Tsuyoshi 
Published: June 2024

AbstractSchizophyllum commune, a common wood-decay mushroom known for its extremely high genetic diversity and as a rare cause of human respiratory diseases, could be a promising model fungus contributing to both biology and medicine. To better understand its phenotypic diversity, we developed an image analysis system that quantifies whole morphological traits of mycelia in Petri dishes. This study evaluated growth of six wild and one clinical isolates of JapaneseS. commune, subjected to different temperatures and glucose concentrations, including a condition mimicking the human respiratory environment. Our analysis revealed that combinations of two growth indices, area and whiteness, highlighted strain-specific responses, with profiling growth patterns using clustering algorithms. Notably, the clinical isolate exhibited the strongest whiteness under the respiratory-like condition. We also found that the growth rate was strongly determined by glucose concentration, while the effects of temperature on growth varied among the strains, suggesting that while glucose preference is common in this species, responses to temperature differ between strains. Our results suggest that the system possesses sufficient sensitivity to detect growth traits of mycelia. This study provides a key to unravelling unknown traits behind the high polymorphisms inS. commune, including the ability to colonize the human respiratory tract.
Multicenter Analysis on Morbidity, Mortality, and Medical Care in Neonates at the Hospital Gineco Obstétrico Isidro Ayora, Hospital General Docente de Calderón, and Hospital Gineco Obstétrico Pediátrico de Nueva Aurora Luz Elena Arismendi, January 2008 - June 2024, Quito-Ecuador (clinical research design protocol)
Authors: Vasco-Morales Santiago , Yánez Valencia Mercedes Elina , Toapanta-Pinta Paola 
Published: June 2024

AbstractIntroductionIn 1983, the Latin American Center for Perinatology/Women’s Health and Reproductive Health published the Perinatal Information System, which records perinatal information of the mother and newborn. At the Isidro Ayora Gynecological Obstetric Hospital, there has been an electronic record of this database since 2008. In 2016, the Ministry of Public Health adapted and added registration variables to this medical history. Similarly, at the General Teaching Hospital of Calderón and the Pediatric Gynecological Obstetric Hospital of Nueva Aurora Luz Elena Arismendi, there are records of neonatal care such as neonatal anthropometry, perinatal risk factors, admission diagnoses, and length of hospital stay. These variables can be integrated with the database of the Isidro Ayora Gynecological Obstetric Hospital.ObjectivesAnalyze the variables of the perinatal clinical history to identify factors associated with changes in fetal-neonatal morbidity and mortality.MethodsDescriptive, analytical, observational study using secondary sources. Frequentist and Bayesian statistical analyses will be applied. To determine the association between qualitative variables, the Chi-square test and logistic regression models will be used. The t-test and linear regression will be used to analyze numerical variables. Statistical significance will be set at p<0.05, and Odds Ratios with a 95% confidence interval will be calculated. Neonatal growth curves stratified by sex and gestational age will be developed. The statistical program R will be used.Expected ResultsThe characteristics of the population served in the hospitals in the north, central, and south of Quito, as well as the demographic and obstetric data of the mothers and their neonates, will be known. Perinatal characteristics associated with an increase or decrease in neonatal morbidity and mortality will be identified. Trends in maternal and child health will be detected and compared with national and international standards.
Needle-Plug/Piston-Based Modular Mesoscopic Design Paradigm Coupled with Microfluidic Device for Large-scale Point-of-Care Pooled Testing
Authors: Lin Baobao , Li Bao , Zeng Wu , Zhao Yulan , Li Huiping , Gu Yin , Liu Peng 
Published: June 2024

AbstractEmerging diagnostic scenarios, such as population surveillance by pooled testing and on-site rapid diagnosis, highlight the importance of advanced microfluidic systems for in vitro diagnostics. However, the widespread adoption of microfluidic technology faces challenges due to the lack of standardized design paradigms, posing difficulties in managing macro-micro fluidic interfaces, reagent storage, and complex macrofluidic operations. This paper introduces a novel modular-based mesoscopic design paradigm, featuring a core “needle-plug/piston” structure with versatile variants for complex fluidic operations. These structures can be easily coupled with various microfluidic platforms to achieve truly self-contained microsystems. Incorporated into a “3D extensible” design architecture, the mesoscopic design meets the demands of function integration, macrofluid manipulations, and flexible throughputs for point-of-care nucleic acid testing. Using this approach, we developed an ultra-sensitive nucleic acid detection system with a limit of detection of 10 copies of SARS-CoV-2 per mL. This system efficiently conducts large-scale pooled testing from 50 pharyngeal swabs in a tube with an uncompromised sensitivity, enabling a truly “sample-in-answer-out” microsystem with exceptional performance.
Service availability, readiness and health-seeking behavior of gender based violence survivor services in primary healthcare facilities in Dodoma, Tanzania. An Explanatory-sequential study design
Authors: Mwamasangula Johnson W. , Gibore Nyasiro S. 
Published: June 2024

AbstractBackgroundThe health sector is a key stakeholder in GBV national response network however, delivery of quality healthcare services for GBV survivors is highly neglected.MethodsThis study will use a mixed-research approach by employing an explanatory sequential study design. Phase one will involve a cross-sectional survey of 61 primary healthcare facilities to examine availability and readiness of GBV survivor’s services. The second phase will involve a descriptive qualitative study among healthcare providers, community healthcare workers and clients to examine the health-seeking behavior and challenges in providing and receiving GBV survivor’s services in primary healthcare facilities. The study will be conducted between March-April 2024 in Dodoma City Council, Tanzania.DiscussionThis study will have potential implications to program managers, policy makers and healthcare providers to tailor interventions that will improve the quality of healthcare service delivery especially towards integrated GBV survivor’s services. But also, understanding the health-seeking behavior and challenges associated with access and provision of GBV survivor’s services will help unveiling gaps in the clients’ point of view and the community, their needs for the GBV survivor’s services and enhancing client-healthcare provider relationship.
Effects of selection stringency on the outcomes of directed evolution
Authors: Alpay Berk A. , Desai Michael M. 
Published: June 2024

AbstractDirected evolution makes mutant lineages compete in climbing complicated sequence-function landscapes. Given this underlying complexity it is unclear how selection stringency, a ubiquitous parameter of directed evolution, impacts the outcome. Here we approach this question in terms of the fitnesses of the candidate variants at each round and the heterogeneity of their distributions of fitness effects. We show that even if the fittest mutant is most likely to yield the fittest mutants in the next round of selection, diversification can improve outcomes by sampling a larger variety of fitness effects. We find that heterogeneity in fitness effects between variants, larger population sizes, and evolution over a greater number of rounds all encourage diversification.
Monocytes Mobilized by Gut Neurons Remodel the Enteric Nervous System
Authors: Kurapati Sravya , Shin Changsik , Szabo Krisztina , Liu Yu , Ashraf Azree Zaffran , Koscso Balazs , Dash Chinmayee , Navarro Leonardo , Saha Monalee , Nagaraj Sushma , Wang Wenhui , Zhu Jun , Shulzhenko Natalya , Baer Christina E. , Srinivasan Shanthi , Kulkarni Subhash , Pasricha Pankaj Jay , Peters Lauren A. , Bogunovic Milena 
Published: June 2024

ABSTRACTThe proper organization of the enteric nervous system (ENS) is critical for normal gastrointestinal (GI) physiology. Inflammatory bowel disease (IBD) dysregulates GI physiology, including bowel movements (motility), but in many IBD patients, GI motility disorders persist in remission through a poorly understood pathological process. Here we uncover that post-inflammatory GI dysmotility (PI-GID) stems from structural ENS remodeling driven by a combination of neuronal loss and neurogenesis. Enteric neurons respond to mucosal inflammation by upregulating CCL2 expression and facilitating the recruitment of CCR2+monocytes into the neural myenteric plexus within the intestinal muscle. This is followed by the expansion of monocyte-derived macrophages and their migration into the myenteric ganglia and phagocytosis of neurons. However, excessive recruitment of monocytes results in disproportionate ENS remodeling and PI-GID. The expansion of inflammatory cells is known to promote tissue hypoxia. We find that enteric neurons become hypoxic upon colitis, but hypoxia-induced signaling via HIF1α initiates an adaptation program in enteric neurons to attenuate CCL2 expression and limit monocyte recruitment. We demonstrate that reinforcing HIF1α signaling in enteric neurons prevents PI-GID by reducing colitis-associated monocyte recruitment in the myenteric plexus and protecting against ENS remodeling. In summary, our findings unveil PI-GID pathogenesis and identify a regulatory axis for its prevention.One Sentence SummaryIntestinal mucosal inflammation engages enteric neurons in the inflammatory response leading to neurogenic recruitment of monocytes into the extra-mucosal myenteric plexus followed by pathological structural remodeling of the enteric nervous system by monocyte-derived macrophages.
WNKs regulate mouse behavior and alter central nervous system glucose uptake and insulin signaling
Authors: Jaykumar Ankita B. , Binns Derk , Taylor Clinton A. , Anselmo Anthony , Birnbaum Shari G. , Huber Kimberly M. , Cobb Melanie H. 
Published: June 2024

AbstractCertain areas of the brain involved in episodic memory and behavior, such as the hippocampus, express high levels of insulin receptors and glucose transporter-4 (GLUT4) and are responsive to insulin. Insulin and neuronal glucose metabolism improve cognitive functions and regulate mood in humans. Insulin-dependent GLUT4 trafficking has been extensively studied in muscle and adipose tissue, but little work has demonstrated either how it is controlled in insulin-responsive brain regions or its mechanistic connection to cognitive functions. In this study, we demonstrate that inhibition of WNK (With-No-lysine (K)) kinases improves learning and memory in mice. Neuronal inhibition of WNK enhancesin vivohippocampal glucose uptake. Inhibition of WNK enhances insulin signaling output and insulin-dependent GLUT4 trafficking to the plasma membrane in mice primary neuronal cultures and hippocampal slices. Therefore, we propose that the extent of neuronal WNK kinase activity has an important influence on learning, memory and anxiety-related behaviors, in part, by modulation of neuronal insulin signaling.
The zebrafish as a new model for studying chaperone-mediated autophagy unveils its role in spermatogenesis
Authors: Goguet Maxime , Vélez Emilio J , Schnebert Simon , Dias Karine , Véron Vincent , Depincé Alexandra , Beaumatin Florian , Herpin Amaury , Seiliez Iban 
Published: June 2024

ABSTRACTChaperone-Mediated Autophagy (CMA) is a major pathway of lysosomal proteolysis involved in numerous cellular processes, and whose dysfunction is associated to several pathologies. Initially studied in mammals and birds, recent findings have identified CMA in fish, reshaping our understanding of its evolution across metazoans. Given the exciting perspectives this finding offered, we have now developed the required tools to investigate and functionally asses that CMA function in a powerful fish genetic model: the zebrafish (Danio rerio). After adapting and validating a fluorescent reporter (KFERQ-Dendra2; previously used to track CMA in mammalian cells) in zebrafish primary embryonic cells, we first demonstrated CMA functionality in this fish species. Then, we developed a transgenic zebrafish line expressing the KFERQ-Dendra2 CMA reporter, enabling the real-time tracking of CMA activityin vivo. This model revealed heterogeneous CMA responses within tissues, highlighting the zebrafish as a valuable model for investigating tissue-specific and cell-scale variations in CMA. Moreover, a novel role for CMA has been uncovered, acting as a gatekeeper of sperm cell proteostasis, thereby playing a crucial role in the production of active and high-quality spermatozoa. Overall, these findings emphasize the zebrafish as a pivotal model for advancing our comprehension of the fundamental mechanisms underlying CMA.
A Nonsecretory Antimicrobial Peptide Mediates Inflammatory Organ Damage inDrosophilaRenal Tubules
Authors: Oi Ayano , Nagashima Shun , Shinoda Natsuki , Miura Masayuki , Obata Fumiaki 
Published: June 2024

SummaryAn excessive immune response damages organs, yet its molecular mechanism is incompletely understood. In this study, we usedDrosophilarenal tubules as a model to screen a factor mediating organ damage upon genetic activation of an innate immune Imd signalling pathway. We identified an antimicrobial peptide, Attacin-D (AttD), which causes organ damage upon Imd activation in the Malpighian tubules. Loss of AttD function suppresses most of the pathological phenotypes induced by Imd activation, such as cell death, compensatory stem cell proliferation, bloating of whole animal, susceptibility to a high salt diet, elevation of purine levels, and mortality, without compromising the immune activation. AttD is required for the immune-induced damage specifically in the Malpighian tubules but not the midgut. Interestingly, AttD uniquely lacks the signal peptide and is not secreted out from cells. Suppression of AttD almost completely attenuates mortality induced by gut tumour-induced immune activation. Our study elucidates the mechanistic effector of immune-induced organ damage.
Interaction of sortilin with apolipoprotein E3 enables neurons to use long-chain fatty acids as alternative metabolic fuel
Authors: Greda Anna K , Gomes Jemila P , Zurawska-Plaksej Ewa , Fritsche-Guenther Raphaela , Rudolph Ina-Maria , Telugu Narasimha S , Cömert Cagla , Kirwan Jennifer , Kunz Séverine , Rothe Michael , Diecke Sebastian , Bross Peter , Willnow Thomas E 
Published: June 2024

SUMMARYSortilin (SORT1) is a lipoprotein receptor that shows genome-wide association with hypercholesterolemia, explained by its ability to control hepatic output of lipoproteins. Remarkably,SORT1also shows genome-wide association with Alzheimer disease (AD) and frontotemporal lobe dementia, the most prevalent forms of age-related dementias. Yet, sortilin’s contribution to human brain lipid metabolism and health remains unclear. Using humanized mouse strains and iPSC-based cell models of brain lipid homeostasis, we document that sortilin mediates neuronal uptake of polyunsaturated fatty acids carried by apoE. Internalized lipids are converted into ligands for PPARα, inducing transcription profiles that enable neurons to use long-chain fatty acids as metabolic fuel. This pathway works with apoE3, but is lost with the AD risk factor apoE4, which disrupts sortilin’s endocytic activity. We document a role for the lipoprotein receptor sortilin in metabolic fuel choice in neurons, possibly crucial when supply with glucose is limited, as in the aging brain.
Charmonium
χc0
and
χc2
resonances in coupled-channel scattering from lattice QCD
Authors: Wilson David J. , Thomas Christopher E. , Dudek Jozef J. , Edwards Robert G. ,   for the Hadron Spectrum Collaboration
Published: June 2024

In order to explore the spectrum of hidden-charm scalar and tensor resonances, we study meson-meson scattering with JPC=0++,2++ in the charmonium energy region using lattice QCD. Employing a light-quark mass corresponding to mπ≈391  MeV, we determine coupled-channel scattering amplitudes up to around 4100 MeV considering all kinematically relevant channels consisting of a pair of open-charm mesons or a charmonium meson with a light meson. A single isolated scalar resonance near 4000 MeV is found with large couplings to DD¯, DsD¯s and the kinematically closed D*D¯* channel. A single tensor resonance at a similar mass couples strongly to DD¯, DD¯* and D*D¯*. We compare the extracted resonances to contemporary experimental candidate states, previous lattice results and theoretical modeling. In contrast to several other studies, we do not find any significant feature in the scalar amplitudes between the ground state χc0(1P) and the resonance found around 4000 MeV.

Published by the American Physical Society
2024
Corporate takeover susceptibility and classification shifting
Authors: Zudana Arfian , Opare Solomon 
Published: June 2024

PurposeThis paper examines the effect of firms’ takeover susceptibility on the manipulation of financial statements through classification shifting.Design/methodology/approachThe paper applies ordinary least squares regression (OLS) with fixed effects analyses to a sample of United States listed firms over the period 1992–2014. We use takeover index as a proxy for takeover susceptibility of firms, with high values representing higher takeover susceptibility and lower values representing lower takeover susceptibility.FindingsThe study finds that firms engage in classification shifting through core expenses, suggesting that takeover threats reduce the incentive to manage earnings through classification shifting. We also find that takeover susceptibility improves the monitoring mechanism for firms with low profitability because these firms have greater incentives to engage in classification shifting. Finally, we find that the Sarbanes–Oxley Act strengthens the monitoring mechanism influenced by takeover threats. Overall, the results provide evidence of the important role of takeover susceptibility in mitigating classification shifting. Our results are robust to a battery of sensitivity tests.Practical implicationsThe results emphasise the disciplinary role of the legal environment around corporate takeovers. The study suggests that policymakers and regulators should be cognisant of antitakeover laws which may increase agency conflicts between managers and shareholders and promote managerial self-seeking behaviours such as classification shifting.Originality/valueThe paper highlights the important role of takeover threats as an external governance mechanism to mitigate classification shifting which is detrimental to investors’ value. From prior literature, this study is the first to provide evidence of the effect of takeover threats on classification shifting.
Examining switching intention in professional sport: a push-pull-mooring perspective
Authors: Sato Mikihiro , Tokuyama Tomo , Motoyama Shohgo , Warwick Lance , Deguchi Junko 
Published: June 2024

PurposeDrawing upon a push-pull-mooring model and the literature on behavioral loyalty, this research aims to examine the underlying factors and mechanisms that influence switching intention in the context of professional sport in Japan.Design/methodology/approachUsing a two-wave survey design (pregame and postgame surveys), we collected data from 367 fans of a professional soccer team in Japan. Our data included three push factors (quality, satisfaction, and perceived value of the current favorite team), a pull factor (alternative team attractiveness), a mooring factor (team identification), and behavioral loyalty and switching intention to the alternative team. We used structural equation modeling to test our hypotheses.FindingsA mediation analysis revealed that perceived value of an individual’s current favorite team was negatively associated with behavioral loyalty to an alternative team, whereas attractiveness of the alternative team was positively associated with behavioral loyalty, which, in turn, resulted in fans’ switching intention. Latent moderated structural equation modeling further revealed the negative moderating effect of team identification with the current team on the relationship between behavioral loyalty and switching intention.Originality/valueThe findings provide new evidence about the role of behavioral loyalty in understanding sport consumers’ switching intention and highlight the importance of team identification that can foster a sustainable and long-term relationship between fans and their favorite sport teams.
Heuristic biases influencing individual stock investment decisions: a hybrid fuzzy DELPHI-AHP-DEMATEL approach
Authors: Abhijith R. , Bijulal D. 
Published: June 2024

PurposeStock investing choices of individual investors are predominantly influenced by heuristic biases, leading to sub-optimal choices. Accordingly, this study aims to identify, categorize, validate, prioritize, and find causality among the heuristic biases shaping stock investment decisions of individual investors.Design/methodology/approachThis research offers original contribution by employing a hybrid approach combining fuzzy DELPHI method (FDM), fuzzy analytical hierarchy process (FAHP), and fuzzy decision-making trial and evaluation laboratory (F-DEMATEL) techniques to validate, prioritize, and find causality among the heuristic biases.FindingsTwenty sub-heuristic biases were identified under five main heuristic bias categories. Out of which, 17 were validated using FDM. Further, availability and representativeness within main heuristic categories, and availability cascade and retrievability within sub-heuristic biases were prioritized using FAHP. Overconfidence and availability were identified as the causes among the five main biases by F-DEMATEL.Practical implicationsThis study offers the stock investors a deeper understanding of heuristic biases and empowers them to make rational investment decisions.Originality/valueThis paper is the inaugural effort to identify, categorize, validate, prioritize and examine the cause-and-effect relationship among the heuristic biases.
The effects of ethical leadership, help seeking and happiness on innovation: an examination in China
Authors: Xie Lei , Li Guangping , Bian Xinyi 
Published: June 2024

PurposeThe study specifically focused on ethical leadership and help seeking during innovation and tested the moderating effect of happiness on the path from help seeking to innovation.Design/methodology/approachIn this longitudinal study, 472 employees from an HR outsourcing company in China participated in the first survey. However, only 184 employees completed two surveys (1 month apart). We used path analysis to test all hypotheses through Mplus.FindingsWe found that ethical leadership, through help-seeking, is correlated to innovation. We also found that one’s happiness level is related to innovation, and when employees are chronically happy, the positive effect of help-seeking on innovation is stronger.Originality/valueWorking in an ethical environment, employees are comfortable acknowledging mistakes, hearing feedback and making corrections, which fosters continuous innovation. Conversely, without the influence from ethical leaders, employees may choose to remain silent and abandon continuous innovation due to the concern of losing “face” the context of China.