bluuebunny/crossref_metadata_2025_split huggingface dataset:
Development of a Double‐inoculation Method to Assess Resistance to Anthracnose in Trispecies Capsicum Hybrid
Authors: Temiyakul Patiporn , Taylor Paul W. J. , Mongkolporn Orarat 
Published: January 2010

AbstractChilli anthracnose is caused by a complex of Colletotrichum species. Breeding for resistance to anthracnose has been focused on introgressing resistance from Capsicum chinense and C. baccatum into commercial C. annuum cultivars. Trispecies hybrids of C. annuum cv. Bangchang, C. chinense PBC932 and C. baccatum PBC80 were successfully produced. Assessments for resistance in F1 progeny to Colletotrichum capsici isolate 158ci (Cc158ci) and C. acutatum isolate MJ5 (CaMJ5) were carried out by inoculating fruit using a laboratory microinjection method. Due to the poor fruit set of the F1 hybrid, a double‐inoculation method was developed to inoculate the same chilli fruit with two isolates of two Colletotrichum species. The positions (apex, centre, end) at which the fruit were inoculated with either isolate did not affect disease development. At 7 days after inoculation, Cc158ci produced larger lesions on chilli fruit than CaMJ5; and lesions from single inoculation were larger than those from double inoculation. The double‐inoculation technique was applied to the trispecies F1 hybrid to select individual F1 plants that contained resistance to both Colletotrichum species. Of the nine F1 plants that produced fruits for inoculation, all were resistant to Cc158ci at both mature green and ripe fruit stages. Two plants were also resistant to CaMJ5 at both fruit maturity stages, and one plant was resistant at the ripe fruit stage but susceptible at the green fruit stage.
Isolated symptomatic cutaneous disease in hypereosinophilic syndrome
Authors: Preda Veronica , Henderson Christopher , Woods Jane 
Published: January 2010

ABSTRACTA 41‐year‐old Phillipino man presented with a 3‐year history of a relapsing and remitting generalized chronic pruritic erythematous papular and plaque‐like eruption. Investigations showed a persistently elevated eosinophil count. His disease was limited to cutaneous involvement with an absence of demonstrable internal organ involvement, despite extensive investigations and multidisciplinary review. Other causes of eosinophilia were excluded. A diagnosis of idiopathic hypereosinophilic syndrome was made. Our patient's presentation raises a number of issues related to hypereosinophilic syndrome. In particular, relating to managing hypereosinophilic syndrome and the challenge of minimizing therapy side‐effects. Our case highlights the considerable morbidity of untreated isolated cutaneous disease, for which he was hospitalized with suicidal ideations. In a minority of reports, skin involvement is the only manifestation of hypereosinophilic syndrome.
Silicification of pelitic schist in the Ryoke low‐pressure/temperature metamorphic belt, Southwest Japan: Origin of competent layers in the middle crust
Authors: Terabayashi Masaru , Yamamoto Hiroshi , Hiwatashi Eri , Kitajima Kouki 
Published: January 2010

AbstractWe propose that competent layers composed of silicified rocks in the Cretaceous Ryoke low‐pressure/temperature (low‐P/T) metamorphic belt in the Iwakuni–Yanai area, Southwest Japan, may be an exhumed equivalent of a seismically‐reflective bright‐layer in the middle crust. Silicified rocks crop out as layers or long lenticular bodies several to fifteen meters in thickness, and they are restricted to the area of greenschist facies conditions within a structural thickness of about one kilometer. The silicified rock layers exhibit a sharp contact with subjacent biotite schist but a gradational contact with overlying pelitic schist. Silicified rock is mainly composed of fine‐grained quartz and minor muscovite and biotite, and some colored minerals have been variably decolorized by alteration. This implies that silicification turned the color of pelitic schists to pale gray or milky white. Quartz veins cutting schistosity at high angles were preferentially developed in the silicified rocks, while schistosity‐parallel quartz veins, which underwent viscous flow, were observed in the underlying biotite schists. En echelon quartz veins and fishnet‐like quartz veins are characteristic of silicified rocks. The modes of occurrence of quartz veins indicate that silicified rocks were competent relative to underlying biotite schist. The combination of contact with high‐competence contrast adjacent to low‐permeability silicified rocks may be a good reflector of seismic waves. If the silicified rocks were distributed in the middle crust to a considerable extent, they may result in seismic bright‐layer reflections.
Osteopontin, a single marker for predicting the prognosis of patients with tumor‐node‐metastasis stage I hepatocellular carcinoma after surgical resection
Authors: Chen Rong‐Xin , Xia Yun‐Hong , Cui Jie‐Feng , Xue Tong‐Chun , Ye Sheng‐Long 
Published: January 2010

AbstractBackground and Aim:  Osteopontin (OPN) has been linked to clinical outcomes in several solid tumors. However, it has not been fully evaluated whether OPN could be used as a single marker for the prognosis of patients with hepatocellular carcinoma (HCC), particularly in patients of the tumor‐node‐metastasis (TNM) stage I.Methods:  A total of 151 patients with HCC who underwent surgical resection were enrolled, including 112 patients of the TNM stage I. OPN expression was evaluated using immunohistochemistry in the tissue microarrays derived from these patients. Immunoreactivity was classified according to the percentage and intensity of staining: negative (−), weak (+) and strong (++). The impact of OPN expression on survival of patients was analyzed.Results:  In total, 65.6% (99 of 151) of HCC tissues expressed OPN. Overall survival in patients of OPN (−) group was significantly higher than those of OPN (+) or OPN (++) group (P = 0.049 and P = 0.001). Interestingly, in patients of the TNM stage I, OPN expression was correlated with the early recurrence after surgical resection (P = 0.001). Multivariate analysis showed that OPN expression was an independent prognostic factor for overall survival and disease‐free survival in patients with the TNM stage I HCC (hazard ratio, 2.272, P = 0.014 and 1.982, P = 0.037).Conclusions:  These results suggest that OPN is commonly expressed in HCC and is a useful marker for predicting the prognosis of patients with the TNM stage I HCC, contributing to determining which individual patient needs adjuvant therapy to prevent the early recurrence after surgical resection.
Childhood minimally invasive follicular carcinoma: Clinical features and immunohistochemistry analysis
Authors: Zou Chao Chun , Zhao Zheng Yan , Liang Li 
Published: January 2010

Aim:  To report on two cases of childhood thyroid minimally invasive follicular carcinoma (MIFC) to highlight the clinical features, laboratory findings and diagnosis of this rare disease.Methods:  The patients' age, gender, clinical features, laboratory findings, pathology and therapy were reviewed. Immunohistochemistry analysis was performed on the resected masses section.Results:  From 2000 to 2008, a total of 15 cases of thyroid cancer were confirmed by pathological analysis, which account for about 2.16% of all malignant solid tumours. They included nine of thyroid papillary carcinoma, two of MIFC and one of undifferentiated thyroid carcinoma. For the two children with MIFC, one was an 8‐year‐boy and one was a 12‐year‐old girl. Thyroid mass was found as the primary sign. Imaging findings showed well‐defined heterogeneous mass and radionuclide scintigraphy with 99mTc demonstrated small cold nodules in the right lobe of thyroid in two cases. Histopathology confirmed the diagnosis of MIFC. Immunohistochemical staining was positive for thyroglobulin, thyroid transcription factor‐1, galectin‐3, Hector Battifora mesothelial antigen‐1, cytokeratin‐AE1/AE3, cytokeratin‐19, proliferating cell nuclear antigen and E‐cadherin in two cases, and S‐100 in one case, while CD56, vimentin and desmin were negative. One case was undertaken lobectomy and the other was undertaken subtotal thyreoidectomy with L‐T4 replacement therapy.Conclusion:  MIFC is exceedingly rare in children and should be included in the differential diagnosis of thyroid mass. The diagnosis of MIFC depends mainly on the pathological findings.
Ultrasonography findings and symptoms in acute rotavirus gastroenteritis
Authors: Yamashita Yasuhiro , Yuge Ken , Tanaka Nobuo , Maeda Kohji , Yamakawa Ryo‐ichi , Matsuyuki Makado , Fsujisawa Takuji , Kimura Akihiko 
Published: January 2010

AbstractBackground:  The aim of the present study was to better understand how clinical events in acute rotavirus gastroenteritis are depicted on ultrasonography.Methods:  The relationship between abdominal ultrasonography and clinical course and symptoms was evaluated in 92 patients with acute rotavirus gastroenteritis (51 boys, 41 girls) with an average age of 30.7 ± 24.6 months.Results:  Significant correlations were observed between ultrasonography score and duration of illness after ultrasonography (P= 0.0232). It was also interesting that ultrasonography findings (score: 3.1 ± 1.0 vs 3.6 ± 1.2; P= 0.0118), clinical symptoms (3.1 ± 1.7 score vs 3.8 ± 1.8 score, P= 0.0293), and duration of illness after ultrasonography (4.4 ± 1.4 days vs 3.8 ± 1.1 days, P= 0.0301) were significantly different between patients examined on ultrasonography <1 day after illness onset (n= 50, but duration of illness after ultrasonography followed only in 42) and those examined ≥2 days after onset (n= 42, but duration of illness after ultrasonography followed only in 35).Conclusion:  The ability of abdominal ultrasonography to indicate severity of disease makes abdominal ultrasonography an important guide to therapy.
Detection of p16 promoter methylation in premature rats with chronic lung disease induced by hyperoxia
Authors: Yue Xiaohong , Fu Jianhua , Xue Xindong , Gao Hong , Liu Dongyan , Zong Zhihong , Wang Wei , Li Hui , Yuan Zhengwei 
Published: January 2010

AbstractBackground:  The aim of the present study was to investigate p16 promoter methylation in premature rats with chronic lung disease (CLD) induced by hyperoxia.Methods:  Eighty Wistar rats were randomized into the hyperoxia group (fraction of inspired oxygen [FiO2]= 900 mL/L) or the control group (FiO2= 210 mL/L), 40 for each group. Semi‐nested methylation‐specific polymerase chain reaction (sn‐MSP) was applied to detect p16 promoter hypermethylation in lung tissues. Additionally, p16 mRNA and protein expression was detected on reverse transcription–polymerase chain reaction (RT‐PCR), western blot and the strept actividin–biotin complex method.Results:  Extended exposure to hyperoxia led to increased methylation, and the methylation level reached a peak in the period of maximum pulmonary fibrosis in the hyperoxia group, while the methylation did not occur in the control group. The methylation rates on semi‐nested PCR (sn‐PCR) and nested‐MSP were, respectively, 52.5% and 42.5% in the hyperoxia group. There was no statistically significant difference between the two methods. The p16 mRNA and protein expression was significantly higher in those with p16 promoter hypermethylation than those without.Conclusion:  Exposure to hyperoxia may induce p16 promoter hypermethylation in lung tissues in premature rats, and methylation risk increases as exposure extends. p16 promoter methylation induced by hyperoxia may be one of the mechanisms for low p16 mRNA and protein expression.
Aberrantly increased mRNA expression of betaglycan, an inhibin co‐receptor in the ovarian tissues in women with polycystic ovary syndrome
Authors: Zhu Rui , Zhou Xiping , Chen Youguo , Qiu Chenqiao , Xu Wenxin , Shen Zongji 
Published: January 2010

AbstractAim:  To compare the gene expressional levels of receptors for activins and inhibins in ovarian tissues between women with polycystic ovary syndrome (PCOS) and normal controls, and to analyze their biologically relevant associations with serum hormone levels.Methods:  Total RNA of ovarian tissues from PCOS (n = 14) and normal controls (n = 21) were isolated during the follicular phase of the menstrual cycle. Real‐time PCRs were performed to examine the relative mRNA expression levels of the activin receptors, including activin receptor type IA (ActRIA), type IB (ActRIB), type IIA (ActRIIA), and type IIB (ActRIIB), and the inhibin receptors, betaglycan and an inhibin binding protein (InhBP/p120). At the same time, the serum levels of estradiol (E2), testosterone (T), follicle‐stimulating hormone (FSH), luteinizing hormone (LH), inhibin B and activin A were measured.Results:  The PCOS patients showed endocrine characteristics with higher concentrations of LH, T and inhibin B, and a lower concentration of activin A. Real‐time PCR demonstrated that the relative expression level of betaglycan against that of GAPDH was increased 1.5‐fold in the ovarian tissues during the follicular phase of PCOS patients when compared with normal controls, while the activin receptors ActRIA, ActRIB, ActRIIA, ActRIIB, and the inhibin co‐receptor InhBP/p120 were unchanged. Moreover, the betaglycan mRNA expression showed biologically relevant associations with serum FSH, LH, E2 and inhibin B levels in both the PCOS and normal controls.Conclusions:  This is the first report to demonstrate the aberrantly increased expression of betaglycan mRNA in PCOS ovaries. The mechanism by which betaglycan contributes to the pathologic process of PCOS remains to be clarified.
Risk factors and algorithms for chlamydial and gonococcal cervical infections in women attending family planning clinics in Thailand
Authors: Rugpao Sungwal , Rungruengthanakit Kittipong , Werawatanakul Yuthapong , Sinchai Wanida , Ruengkris Tosaporn , Lamlertkittikul Surachai , Pinjareon Sutham , Koonlertkit Sompong , Limtrakul Aram , Sriplienchan Somchai , Wongthanee Antika , Sirirojn Bangorn , Morrison Charles S. , Celentano David D. 
Published: January 2010

AbstractAim:  To identify risk factors associated with and evaluate algorithms for predicting Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) cervical infections in women attending family planning clinics in Thailand.Methods:  Eligible women were recruited from family planning clinics from all regions in Thailand. The women were followed at 3‐month intervals for 15–24 months. At each visit, the women were interviewed for interval sexually transmitted infection (STI) history in the past 3 months, recent sexual behavior, and contraceptive use. Pelvic examinations were performed and endocervical specimens were collected to test for CT and NG using polymerase chain reaction.Results:  Factors associated with incident CT/NG cervical infections in multivariate analyses included region of country other than the north, age ≤25 years, polygamous marriage, acquiring a new sex partner in the last 3 months, abnormal vaginal discharge, mucopurulent cervical discharge, and easily induced bleeding of the endocervix. Three models were developed to predict cervical infection. A model incorporating demographic factors and sexual behaviors had a sensitivity of 61% and a specificity of 71%. Incorporating additional factors did not materially improve test performance. Positive predictive values for all models evaluated were low.Conclusion:  In resource‐limited settings, algorithmic approaches to identifying incident cervical infections among low‐risk women may assist providers in the management of these infections.
Vaginal intraepithelial neoplasia: A retrospective analysis of clinical features and colpohistology
Authors: Boonlikit Sathone , Noinual Nattakul 
Published: January 2010

AbstractAim:  To analyze the clinical features and colpohistology of women with vaginal intraepithelial neoplasia (VAIN).Methods:  Patients with VAIN treated at Rajavithi Hospital between April 1986 and October 2007.Results:  Of 68 patients, 52.9% had VAIN2/3. The mean age of patients was 50.85 ± 13.17 years (range: 22–80). The most common presentation was abnormal cytology. History of prior pelvic radiation, associated neoplasia of the lower genital tract and history of prior hysterectomy were documented in 7.4, 67.6 and 54.4% of patients, respectively. Median intervals from previous lower genital tract neoplasia to current diagnosis of VAIN, and between hysterectomy to diagnosis of VAIN were 23.9 and 29.7 months, respectively. The upper third of the vagina was the most common site of VAIN (86.8%). The most common colposcopic finding was the presence of acetowhite epithelium. Punctation was significantly associated with VAIN2/3 (P < 0.05). Mosaic was rare and found in VAIN3.Conclusion:  VAIN can occur at any age and is commonly associated with neoplasia of the lower genital tract. Most patients have disease located in the upper third of the vagina. The most common colposcopic finding is acetowhite epithelium while punctation and mosaic pattern are more prevalent in VAIN2/3.
Tumor necrosis factor α, interleukin‐6 and interleukin‐10 polymorphisms in preeclampsia
Authors: Vural Pervin , Degirmencioglu Sevgin , Saral Neslihan Y. , Demirkan Ayse , Akgul Cemil , Yildirim Gokhan , Issever Halim , Eroglu Hacer 
Published: January 2010

AbstractAim:  Preeclampsia (PE) is one of the most serious disorders of pregnancy. The imbalance between pro‐ and anti‐inflammatory cytokines may play a role in its etiology. The aim of the present study was to investigate whether cytokine gene polymorphism is associated with PE, and to evaluate the relationship between genotypes and clinical/laboratory manifestation of PE.Methods:  We investigated single nucleotide polymorphisms of tumor necrosis factor (TNF)α(‐308 G/A), interleukin (IL)‐6 (‐174 G/C), IL‐10 (‐1082 G/A) genes in DNA from peripheral blood leukocytes of 101 PE patients and 95 healthy control women.Results:  In PE, there was a significant increase of the IL‐10 (‐1082) A allele frequency (P = 0.04). No significant differences were found in genotypes or allele frequencies of TNFα(‐308) and IL‐6 (‐174) genes between PE women and controls. While TNFα(‐308) and IL‐6 (‐174) genotypes did not influence clinical/laboratory parameters in PE, IL‐10 (‐1082) A allele carrying genotypes (AG + AA) were associated with higher glucose and lower HDL‐cholesterol levels.Conclusion:  Because women with IL‐10 (‐1082) AA genotype have 3.38‐fold increased risk of developing PE according to GG genotype (95% CI 1.21–9.4, P = 0.01), we suggest that IL‐10 (‐1082) variant A allele is associated with an increased risk of preeclampsia, which is independent from its metabolic effects.
Feasibility and safety of total laparoscopic hysterectomy (TLH) using the Hohl instrument in nonobese and obese women
Authors: Mueller Andreas , Thiel Falk , Lermann Johannes , Oppelt Peter , Beckmann Matthias W. , Renner Stefan P. 
Published: January 2010

AbstractAim:  The aim of the present study was to evaluate the feasibility, safety, and complication rates of total laparoscopic hysterectomy (TLH) using the Hohl instrument in nonobese and obese women.Methods:  A prospective controlled trail (Canadian Task Force classification II‐1) was conducted at the Department of Obstetrics and Gynecology, Erlangen University Hospital, Erlangen‐Nuremberg, Germany. Two hundred and fifty‐seven women underwent total laparoscopic hysterectomy using the Hohl instrument between January 2006 and May 2008. The laparoscopic approach was used when the patient had undergone previous pelvic abdominal operation and/or had a reduced vaginal capacity. Women were classified according to their body mass index (BMI), with a cutoff point of 30 kg/m2. Total laparoscopic hysterectomy was performed using the Hohl instrument.Results:  One ureteral injury, one bladder injury, one vaginal injury, and one injury to the epigastric vessels occurred. Blood transfusion was necessary in one patient. One conversion to abdominal hysterectomy was performed in a woman with a uterus weighing 893 g. In the postoperative phase, suture dehiscence at the vaginal closure occurred in three patients, one patient had bladder infection, one woman developed unexplained fever, and a hematoma without infection at the vaginal vault occurred in one woman. All complications occurred in women with BMI < 30 kg/m2.Conclusion:  TLH using the Hohl instrument is feasible and safe in nonobese and obese patients.
Basal ganglia control of sleep–wake behavior and cortical activation
Authors: Qiu Mei‐Hong , Vetrivelan Ramalingam , Fuller Patrick M. , Lu Jun 
Published: January 2010

AbstractThe basal ganglia (BG) are involved in numerous neurobiological processes that operate on the basis of wakefulness, including motor function, learning, emotion and addictive behaviors. We hypothesized that the BG might play an important role in the regulation of wakefulness. To test this prediction, we made cell body‐specific lesions in the striatum and globus pallidus (GP) using ibotenic acid. We found that rats with striatal (caudoputamen) lesions exhibited a 14.95% reduction in wakefulness and robust fragmentation of sleep–wake behavior, i.e. an increased number of state transitions and loss of ultra‐long wake bouts (> 120 min). These lesions also resulted in a reduction in the diurnal variation of sleep–wakefulness. On the other hand, lesions of the accumbens core resulted in a 26.72% increase in wakefulness and a reduction in non‐rapid eye movement (NREM) sleep bout duration. In addition, rats with accumbens core lesions exhibited excessive digging and scratching. GP lesions also produced a robust increase in wakefulness (45.52%), and frequent sleep–wake transitions and a concomitant decrease in NREM sleep bout duration. Lesions of the subthalamic nucleus or the substantia nigra reticular nucleus produced only minor changes in the amount of sleep–wakefulness and did not alter sleep architecture. Finally, power spectral analysis revealed that lesions of the striatum, accumbens and GP slowed down the cortical electroencephalogram. Collectively, our results suggest that the BG, via a cortico‐striato‐pallidal loop, are important neural circuitry regulating sleep–wake behaviors and cortical activation.
Advanced paternal age is associated with alterations in discrete behavioural domains and cortical neuroanatomy of C57BL/6J mice
Authors: Foldi Claire J. , Eyles Darryl W. , McGrath John J. , Burne Thomas H. J. 
Published: January 2010

AbstractAdvanced paternal age (APA) is associated with an increased risk of neurodevelopmental disorders such as autism and schizophrenia. A previous study in mice suggested that the offspring of aged sires have altered locomotion and avoidance learning. The aim of the current study was to conduct a comprehensive behavioural screen in adult offspring of mice of APA. We also examined brain morphology in neonate and adult mice. The adult offspring of 12‐ to18‐month‐old (APA) and 4‐month‐old (control) male C57BL/6J mice underwent a behavioural test battery comprising tests for locomotion, anxiety, exploration, social behaviour, learned helplessness and sensorimotor gating. The brains of these mice were collected at 3 months and imaged ex vivo using a 16.4T MRI scanner to assess gross neuroanatomy. Neuroanatomy was also examined at birth in a separate cohort of animals. Overall, the APA mouse model was associated with subtle behavioural changes and altered cortical morphology. The behavioural phenotype of female APA mice included increased anxiety‐related behaviour, increased exploration and decreased learned helplessness compared to control females. Male APA mice had thinner cortices at birth and increased cortical volume as adults. This animal model may assist in exploring the mechanism of action linking APA with disorders such as schizophrenia and autism.