bluuebunny/crossref_metadata_2025_split huggingface dataset:
Annexin A1 is associated with gastric cancer survival and promotes gastric cancer cell invasiveness through the formyl peptide receptor/extracellular signal‐regulated kinase/integrin beta‐1‐binding protein 1 pathway
Authors: Cheng Tsu‐Yao , Wu Ming‐Shiang , Lin Jaw‐Town , Lin Ming‐Tsan , Shun Chia‐Tung , Huang Hsin‐Yi , Hua Kuo‐Tai , Kuo Min‐Liang 
Published: June 2012

AbstractBACKGROUND:Annexin A1 (AnxA1) has been well‐known as a glucocorticoid‐regulated anti‐inflammatory protein, and it is implicated in tumorigenesis in a tumor type–specific pattern. However, the role of AnxA1 in gastric cancer (GC) is indeterminate, and the underlying mechanism is not clear. The purpose of this study was to evaluate the prognostic significance and associated mechanism of AnxA1 in GC.METHODS:Immunohistochemical staining was employed to analyze 118 GC patients. Both AnxA1 gain‐of‐function and loss‐of‐function approaches were performed in GC cells. Western blotting and reverse‐transcription polymerase chain reaction were used for assessment of the AnxA1 regulation mechanism in GC cells. An intraperitoneal inoculation model in severe combined immunodeficient mice was used for an in vivo assay.RESULTS:High AnxA1 expression was significantly associated with peritoneal metastasis (P = .009) and serosal invasion (P = .044). Cox multivariate analysis showed that high AnxA1 expression was an independent risk factor for poor overall survival in GC patients (P = .037). AnxA1 expression positively correlated with invasiveness of human GC cells both in vitro and in vivo. AnxA1 could regulate the GC cell invasion through the formyl peptide receptor (FPR)/extracellular signal‐regulated kinase/integrin beta‐1‐binding protein pathway, and all 3 FPRs (FPR1 through FPR3) were involved in the regulation process.CONCLUSIONS:High AnxA1 expression was associated with more serosal invasion, more peritoneal metastasis, and poorer overall survival in GC patients. The current study demonstrated a novel mechanism involving FPRs, extracellular signal‐regulated kinases 1 and 2, and integrin beta‐1‐binding protein 1 by which AnxA1 regulated GC cell invasion. Cancer 2012. © 2012 American Cancer Society.
Clinical implications and diagnostic usefulness of correlation between soluble major histocompatibility complex class I chain‐related molecule a and protumorigenic cytokines in pancreatic ductal adenocarcinoma
Authors: Chung Hye Won , Jang Sunphil , Lim Jong‐Baeck 
Published: June 2012

AbstractBACKGROUND:Tumor‐derived soluble factors serve as mediators between tumors and surrounding microenvironment to promote tumor growth and metastasis under a complex network. The objective of this study was to evaluate the relationships between soluble major histocompatibility complex class I chain‐related molecule A (sMICA) and 4 categories of cytokines (tumor‐related proinflammatory, anti‐inflammatory, chemotactic/proangiogenic, and growth‐stimulatory) in the development and progression of pancreatic ductal adenocarcinoma (PDAC).METHODS:Serum levels of sMICA and 4‐categorized cytokines were measured by enzyme‐linked immunosorbent assay and chemiluminescent immunoassay, respectively, in 134 individuals (normal, n = 55; chronic pancreatitis, n = 25; PDAC, n = 54). Clinical implications of sMICA and tumor‐related cytokines, their correlations, and diagnostic usefulness in PDAC were evaluated.RESULTS:Serum sMICA, which was associated with the development and progression of PDAC, correlated with interferon‐γ negatively (P = 0.024), whereas it correlated positively with the anti‐inflammatory cytokines interleukin‐10 (IL‐10) and IL‐1 receptor antagonist, and the bifunctional cytokine tumor necrosis factor α, with respect to PDAC development (P < .05). sMICA also correlated positively with the chemotactic/proangiogenic cytokines vascular endothelial growth factor, soluble CD40 ligand, and IL‐8, and the tumor growth‐stimulatory cytokines epidermal growth factor and transforming growth factor α, with respect to PDAC development and/or progression. Logistic regression analysis validated the diagnostic usefulness of combination use of sMICA and its related cytokines to predict the presence of PDAC and distant metastasis in PDAC, superior to carbohydrate antigen 19‐9.CONCLUSIONS:sMICA may be involved in tumor‐associated angiogenesis and tumor growth either directly or indirectly by affecting corresponding cytokines as well as causing impairment of natural killer cell cytotoxicity in the development and progression of PDAC. A combination of sMICA and its related cytokines exhibited remarkable diagnostic potential in PDAC. Cancer 2013. © 2012 American Cancer Society.
Influence of FLT3‐internal tandem duplication allele burden and white blood cell count on the outcome in patients with intermediate‐risk karyotype acute myeloid leukemia
Authors: How Jonathan , Sykes Jenna , Gupta Vikas , Yee Karen W. L. , Schimmer Aaron D. , Schuh Andre C. , Minden Mark D. , Kamel‐Reid Suzanne , Brandwein Joseph M. 
Published: June 2012

AbstractBACKGROUND:In patients with acute myeloid leukemia (AML), testing for fms‐like tyrosine kinase‐3 (FLT3)–internal tandem duplication (FLT3‐ITD) and nucleophosmin‐1 (NPM1) mutations can allow for further prognostic subclassification, but less is known about the effects of FLT3‐ITD allele burden and presenting white blood cell count (WBC) within molecular subgroups.METHODS:The authors retrospectively assessed 206 adult patients who had AML with an intermediate‐risk karyotype and who received treatment on a uniform induction and consolidation chemotherapy regimen.RESULTS:The presenting WBC was a prognostic factor for survival only in patients who had an FLT3‐ITD mutation. On multivariate analysis, after correcting for age, WBC, secondary AML, and blast percentage, nucleophosmin‐1 (NPM1)‐mutated/FLT3‐ITD–negative patients had superior overall survival compared with patients in the other molecular subgroups. Patients who had FLT3‐ITD mutations had an inferior overall survival compared with patients who had NPM1 wild‐type/FLT3‐negative disease, and patients who had low or intermediate levels of the FLT‐ITD of mutant allele had overall and disease‐free survival similar to those in patients who had high‐level mutations.CONCLUSIONS:NPM1 and FLT3‐ITD status, age, WBC, and secondary AML were identified as important prognostic variables that can help to risk stratify patients with AML who have intermediate‐risk cytogenetics. FLT3 allele burden had no significant influence on outcomes after correcting for other variables. Cancer 2012. © 2012 American Cancer Society.
A microRNA expression signature for the prognosis of oropharyngeal squamous cell carcinoma
Authors: Gao Ge , Gay Hiram A. , Chernock Rebecca D. , Zhang Tian R. , Luo Jingqin , Thorstad Wade L. , Lewis James S. , Wang Xiaowei 
Published: June 2012

AbstractBACKGROUND:Oropharyngeal squamous cell carcinoma (SCC) rates have been increasing significantly in recent years, despite a decreasing incidence of head and neck cancer in general. Oropharyngeal SCC has many characteristics that are distinct from other head and neck cancers, and thus it is important to focus specifically on cancers arising in this region, with the goal of improving patient outcomes. One important goal is to identify those patients who are likely to fail standard therapy and who could potentially benefit from alternative or targeted treatments.METHODS:In the current study, the prognostic value of microRNAs (miRNAs) was evaluated in patients with oropharyngeal SCC. miRNAs are small, noncoding RNAs that are master regulators of many important biological processes. In total, 150 oropharyngeal tumors were analyzed using the recently developed quantitative polymerase chain reaction‐based method for miRNA expression profiling. In addition, the expression of miRNAs was also compared with human papillomavirus (HPV) transcriptional activities.RESULTS:The current study identified 6 miRNAs that were found to be significantly associated with cancer survival. A combined expression signature of these miRNAs was prognostic of oropharyngeal SCC, independent of common clinical features or HPV status.CONCLUSIONS:This new miRNA signature was experimentally validated in an independent oropharyngeal SCC cohort. Furthermore, 5 HPV‐related miRNAs were identified, which may help to characterize HPV‐induced cancers including both oropharyngeal and cervical SCC. Cancer 2013. © 2012 American Cancer Society.
Predictive and prognostic roles of ribonucleotide reductase M1 in resectable pancreatic adenocarcinoma
Authors: Xie Hao , Jiang Wei , Jiang John , Wang Yixin , Kim Richard , Liu Xiaobo , Liu Xiuli 
Published: June 2012

AbstractBACKGROUND:Ribonucleotide reductase M1 (RRM1) is an important molecule in different types of cancer. The objective of this study was to evaluate the predictive roles of RRM1 in the survival of patients with resectable pancreatic adenocarcinoma who received treatment with gemcitabine or nongemcitabine adjuvant therapy.METHODS:In total, 122 patients underwent tumor resection for pancreatic adenocarcinoma at the authors' institution from October 1999 to December 2007. Total RNA was isolated from microdissected, paraffin‐embedded tumors. RRM1 expression levels were measured using quantitative reverse transcriptase‐polymerase chain reaction (QRT‐PCR) and were dichotomized using recursive partitioning analysis. The Kaplan‐Meier method was used to estimate overall survival and progression‐free survival, and the predictive value of RRM1 expression on survival was examined using Cox proportional hazards regression.RESULTS:RRM1 expression did not have significant prognostic value in the entire cohort regarding overall survival (P = .2) or progression‐free survival (P = .7). In the subgroup of 44 patients who received adjuvant gemcitabine, patients who had low RRM1 expression had longer overall survival (median, 47.8 months vs 14.1 months; P = .005) and a trend toward longer progression‐free survival (median not reached vs 12.9 months; P = .06). In contrast, in the subgroup of 35 patients who received nongemcitabine adjuvant therapy, patients who had high RRM1 expression had significantly longer overall survival (median, 41.9 months vs 19.8 months; P = .01) and progression‐free survival (median, 70.0 months vs 11.8 months; P = .04). These results were confirmed in Cox proportional hazards multivariable analysis.CONCLUSIONS:In patients with resectable pancreatic adenocarcinoma, low RRM1 expression in the tumor predicted an overall survival benefit of adjuvant gemcitabine; and high RRM1 expression predicted the survival benefit of nongemcitabine adjuvant therapy. Cancer 2013. © 2012 American Cancer Society.
Predictors of locoregional outcome in patients receiving neoadjuvant therapy and postmastectomy radiation
Authors: Wright Jean L. , Takita Cristiane , Reis Isildinha M. , Zhao Wei , Saigal Kunal , Wolfson Aaron , Markoe Arnold , Moller Mecker , Hurley Judith 
Published: June 2012

AbstractBACKGROUND:The objective of this study was to identify predictors of locoregional recurrence (LRR) after neoadjuvant therapy (NAT) and postmastectomy radiation (PMRT) in a cohort of patients with stage II through III breast cancer and to determine whether omission of the supraclavicular field had an impact on the risk of LRR.METHODS:The authors reviewed records from 464 patients who received NAT and PMRT from January 1999 to December 2009.RESULTS:The median patient age was 50 years (range, 25‐81 years). Clinical disease stage was stage II in 29% of patients, stage III in 71%, and inflammatory in 14%. Receptor status was estrogen receptor (ER)‐positive in 54% of patients, progesterone receptor (PR)‐positive in 39%, human epidermal growth factor receptor 2 (HER2)‐positive in 24%, and negative for all 3 receptors (triple negative) in 32%. All patients received NAT and underwent mastectomy, and 19.6% had a complete pathologic response in the breast and axilla, 17.5% received radiation to the chest wall only, and 82.5% received radiation to the chest wall and the supraclavicular field; omission of the supraclavicular field was more common in patients with lower clinical and pathologic lymph node status. The median follow‐up was 50.5 months, and the 5‐year cumulative incidence of LRR was 6% (95% confidence interval, 3.9%‐8.6%). Predictors of LRR were clinical stage III (P = .038), higher clinical lymph node status (P = .025), higher pathologic lymph node status (P = .003), the combination of clinically and pathologically positive lymph nodes (P < .001), inflammatory presentation (P = .037), negative ER status (P = .006), negative PR status (P = .015), triple‐negative status (P < .001), and pathologic tumor size >2 cm (P = .045). On univariate analysis, omission of the supraclavicular field was not associated significantly with LRR (hazard ratio, 0.89; P = .833); however, on multivariate analyses, omission of the supraclavicular field was associated significantly with LRR (hazard ratio, 3.39; P = .024).CONCLUSIONS:Presenting stage, receptor status, pathologic response to neoadjuvant therapy, and omission the supraclavicular field were identified as risk factors for LRR after neoadjuvant therapy and PMRT. Cancer 2013. © 2012 American Cancer Society.
Neurochemical features of boar lumbosacral dorsal root ganglion neurons and characterization of sensory neurons innervating the urinary bladder trigone
Authors: Russo D. , Clavenzani P. , Sorteni C. , Bo Minelli L. , Botti M. , Gazza F. , Panu R. , Ragionieri L. , Chiocchetti R. 
Published: June 2012

AbstractPorcine lumbosacral dorsal root ganglion (DRG) neurons were neurochemically characterized by using six neuronal markers: calcitonin gene‐related peptide (CGRP), substance P (SP), neuronal nitric oxide synthase (nNOS), neurofilament 200kDa (NF200), transient receptor potential vanilloid 1 (TRPV1), and isolectin B4 (IB4) from Griffonia simplicifolia. In addition, the phenotype and cross‐sectional area of DRG neurons innervating the urinary bladder trigone (UBT) were evaluated by coupling retrograde tracer technique and immunohistochemistry. Lumbar and sacral DRG neuronal subpopulations were immunoreactive (IR) for CGRP (30 ± 3% and 29 ± 3%, respectively), SP (26 ± 8% and 27 ± 12%, respectively), nNOS (21 ± 4% and 26 ± 7%, respectively), NF200 (75 ± 14% and 81 ± 7%, respectively), and TRPV1 (48 ± 13% and 43 ± 6%, respectively), and labeled for IB4 (56 ± 6% and 43 ± 10%, respectively). UBT sensory neurons, which were distributed from L2 to Ca1 DRG, had a segmental localization, showing their highest density in L4–L5 and S2–S4 DRG. Lumbar and sacral UBT sensory neurons expressed similar percentages of NF200 immunoreactivity (64 ± 33% and 58 ± 12%, respectively) but showed a significantly different immunoreactivity for CGRP, SP, nNOS, and TRPV1 (56 ± 9%, 39 ± 15%, 17 ± 13%, 62 ± 10% vs. 16 ± 6%, 16 ± 11%, 6 ± 1%, 45 ± 24%, respectively). Lumbar and sacral UBT sensory neurons also showed different IB4 labeling (67 ± 19% and 48 ± 16, respectively). Taken together, these data indicate that the lumbar and sacral pathways probably play different roles in sensory transmission from the UBT. The findings related to cell size also reinforced this hypothesis, because lumbar UBT sensory neurons were significantly larger than sacral ones (1,112 ± 624 μm2 vs. 716 ± 421 μm2). J. Comp. Neurol. 521:342–366, 2013. © 2012 Wiley Periodicals, Inc.
Automatic MRI 2D brain segmentation using graph searching technique
Authors: Pedoia Valentina , Binaghi Elisabetta 
Published: June 2012

SUMMARYAccurate and efficient segmentation of the whole brain in magnetic resonance (MR) images is a key task in many neuroscience and medical studies either because the whole brain is the final anatomical structure of interest or because the automatic extraction facilitates further analysis. The problem of segmenting brain MRI images has been extensively addressed by many researchers. Despite the relevant achievements obtained, automated segmentation of brain MRI imagery is still a challenging problem whose solution has to cope with critical aspects such as anatomical variability and pathological deformation. In the present paper, we describe and experimentally evaluate a method for segmenting brain from MRI images basing on two‐dimensional graph searching principles for border detection. The segmentation of the whole brain over the entire volume is accomplished slice by slice, automatically detecting frames including eyes. The method is fully automatic and easily reproducible by computing the internal main parameters directly from the image data. The segmentation procedure is conceived as a tool of general applicability, although design requirements are especially commensurate with the accuracy required in clinical tasks such as surgical planning and post‐surgical assessment. Several experiments were performed to assess the performance of the algorithm on a varied set of MRI images obtaining good results in terms of accuracy and stability. Copyright © 2012 John Wiley & Sons, Ltd.
Simulation of the Neutral Inventory in the Pilot‐PSI Beam
Authors: Wieggers R. C. , Groen P. W. C. , de Blank H. J. , Goedheer W. J. 
Published: June 2012

AbstractThe Eunomia code is used to study the neutral species in and near a hydrogen plasma beam. Eunomia is a non‐linear Monte Carlo transport code that solves the neutral equilibrium, given a fixed background plasma. The code is developed to study the neutral inventory of Pilot‐PSI and Magnum‐PSI, linear devices developed to study plasma surface interactions in similar conditions as expected in the ITER divertor. Results show the influence of elastic collisions and the outer vessel wall on the neutral species. In the center of the 2 cm diameter Pilot‐PSI beam the results show a strong coupling to the plasma. Only millimeters away from the center, the neutral flow, temperature and density are strongly influenced by recombination processes at the vessel wall (© 2012 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Computation of minimum transmit power for network connectivity in vehicular ad hoc networks formed by vehicles with random communication range
Authors: Neelakantan P.C. , Babu A.V. 
Published: June 2012

SUMMARYIn this paper, we consider a vehicular ad hoc network used for distributing traffic information and investigate the minimum uniform transmit power, to be used by all vehicles, sufficient to guarantee network connectivity, in the presence of channel fading. As opposed to the conventional graph theoretic approach, in this paper, the network connectivity problem is analyzed from a physical layer perspective in which a given multi‐hop path joining a pair of vehicles in the network is considered to be connected if and only if the average route BER corresponding to this path meets a target requirement. The analysis considers the communication range of each vehicle to be a random variable. The random communication range model is relevant for connectivity analysis because it can account for variability in the communication links and thus will be able to accurately estimate the network connectivity probability after network setup. We derive closed form analytical expression for the minimum transmit power sufficient to ensure network connectivity. We also derive analytical expression for the maximum number of hops, a packet can traverse satisfying the route BER constraint, for a given transmit power. The validity of our theoretical analysis is verified by extensive simulation studies. Copyright © 2012 John Wiley & Sons, Ltd.
Identification of magnetizing inrush current phenomena in a distribution system
Authors: Dou Naoki , Toyama Atushi , Satoh Kohki , Naitoh Tadashi , Masaki Kazuyuki 
Published: June 2012

AbstractIn high‐voltage distribution systems, there are many power quality troubles due to voltage dips. Otherwise, magnetizing inrush currents cause voltage dips. To suppress voltage dips, it is necessary to identify magnetizing inrush current phenomena. In this paper, the authors propose a new identification method. The principles are that the saturation start and end flux are equal and the inrush current pattern exists. To avoid interference with saturation area overlap, the rectangular coordinate method is adopted. © 2012 Wiley Periodicals, Inc. Electr Eng Jpn, 181(1): 1–10, 2012; Published online in Wiley Online Library (wileyonlinelibrary.com). DOI 10.1002/eej.21259
First Total Synthesis of the Pyrrolizidine Alkaloid Amphorogynine C through Intramolecular Azide–Olefin Cycloaddition
Authors: de Miguel Irene , Velado Marina , Herradón Bernardo , Mann Enrique 
Published: June 2012

AbstractThe first total synthesis of the natural alkaloid amphorogynine C is reported (2.9 % overall yield in 20 steps). The key steps include a Claisen–Johnson rearrangement and an intramolecular azide–olefin cycloaddition, followed by a reduction of the resulting imine. The construction of the pyrrolizidine skeleton was achieved by an alkoxide‐mediatedlactone ring opening and subsequent cyclization of a conveniently functionalized bicyclic amine. Finally, the proposed structure of amphorogynine C was confirmed by single‐crystal X‐ray diffraction analysis.
Fused Arene Ring Construction Around Pyrrole To Form 4,7‐Disubstitued Indole
Authors: Nojman Elżbieta , Latos‐Grażyński Lechosław , Szterenberg Ludmiła 
Published: June 2012

AbstractA method for synthesizing 4,7‐diarylindoles and 4,7‐di(thien‐2‐yl)indole by applying a strategy where the fused benzene ring was built on an existing pyrrole in an acid‐catalyzed rearrangement of 1,4‐diaryl‐1,4‐di(pyrrol‐2‐yl)but‐2‐yne and 1,4‐di(pyrrol‐2‐yl)‐1,4‐di(thien‐2‐yl)but‐2‐yne, respectively, is presented. In the presence of TFA (30 equiv.), 4,7‐diarylindoles undergo thermodynamically equilibrated dimerization at 240 K as determined by 1H NMR spectroscopy and substantiated by DFT calculations.
Chiral separation of cathinone derivatives used as recreational drugs by cyclodextrin‐modified capillary electrophoresis
Authors: Mohr Stefan , Pilaj Stefan , Schmid Martin G. 
Published: June 2012

In recent years, cathinone derivatives have entered the global drug market and caused serious social problems in many European countries. Modification of the basic structure of cathinone leads to a multitude of derivatives, including the most popular representative mephedrone. All those substances contain a stereogenic center and therefore two isoforms exist. As it is the case with many chiral active pharmaceutical ingredients, even the pharmacological effect of the enantiomers of those psychoactive compounds may differ. During this research, an easy‐to‐prepare chiral capillary zone electrophoresis method for the enantioseparation of a set of 19 cathinone derivatives was developed. Testing different types of cyclodextrin (CD), including native‐β‐CD, carboxymethyl‐β‐CD, 2‐hydroxypropyl‐β‐CD, sulfated‐β‐CD, and native γ‐CD, best results were obtained with the negatively charged sulfated‐β‐CD. The effect of the CD concentration, the temperature, and the addition of ACN to the BGE on the enantioseparation is shown by three model compounds. Under optimal conditions, using 20 mg/mL sulfated‐β‐CD in 50 mM ammonium acetate buffer pH = 4.5 containing 10% v/v ACN at a cassette temperature of 40°C and with an applied voltage of 20 kV, all derivatives except methedrone were resolved in their enantiomers within 20 min.