bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rainbow fringes around crevice corrosion formed on stainless steel AISI 316 after ennoblement in seawater
Authors: Wang W. , Zhang X. , Wang J. 
Published: May 2009

AbstractThe crevice corrosion occurrence probability of stainless steel (SS) AISI 316 was increased under ennoblement condition due to chemically added H2O2 into seawater. The H2O2 was used to simulate the important factor causing ennoblement in natural marine biofilm. Morphology of the crevice corrosion was observed using an incident‐light source microscopy. Some interesting “rainbow” fringes were observed around micro‐crevices. The mechanism was discussed from the ions diffusion and potential distribution during the crevice formation. This result shows that under ennoblement condition the colored fringe is a distinct characteristic of the morphology of localized corrosion for stainless steel.
Regulation of peroxisome proliferator‐activated receptor‐β/δ by the APC/β‐CATENIN pathway and nonsteroidal antiinflammatory drugs
Authors: Foreman Jennifer E. , Sorg Joseph M. , McGinnis Kathleen S. , Rigas Basil , Williams Jennie L. , Clapper Margie L. , Gonzalez Frank J. , Peters Jeffrey M. 
Published: May 2009

AbstractStudies indicate that peroxisome proliferator‐activated receptor‐β/δ (PPARβ/δ) can either attenuate or potentiate colon cancer. One hypothesis suggests that PPARβ/δ is upregulated by the adenomatous polyposis coli (APC)/β‐CATENIN pathway and a related hypothesis suggests that PPARβ/δ is downregulated by nonsteroidal antiinflammatory drugs (NSAIDs). The present study examined these possibilities using in vivo and in vitro models. While APC/β‐CATENIN‐dependent expression of CYCLIN D1 was observed in vivo and in vitro, expression of PPARβ/δ was not different in colon or intestinal polyps from wild‐type or Apcmin heterozygous mice or in human colon cancer cell lines with mutations in APC and/or β‐CATENIN. No difference in the level of PPARβ/δ was found in colon from wild‐type or Apcmin heterozygous mice following treatment with NO‐donating aspirin (NO‐ASA). NSAIDs inhibited cell growth in RKO (wild‐type APC) and DLD1 (mutant APC) human colon cancer cell lines but expression of PPARβ/δ was not downregulated in these cell lines in response to a broad concentration range of celecoxib, indomethacin, NS‐398, or nimesulide. However, indomethacin caused an increase in PPARβ/δ mRNA and protein that was accompanied with increased expression of a known PPARβ/δ target gene. Interestingly, expression of PPARα was also increased in the human colon cancer cell lines by several NSAIDs at the highest concentration examined. Results from these studies provide additional evidence indicating that PPARβ/δ is not upregulated by the APC/β‐CATENIN pathway. Further, these studies suggest that increased PPARβ/δ and/or PPARα by NSAIDs in human colon cancer cell lines could contribute to the mechanisms underlying the chemopreventive effects of NSAIDs. Mol. Carcinog. © 2009 Wiley‐Liss, Inc.
Cyclin D1b variant promotes cell invasiveness independent of binding to CDK4 in human bladder cancer cells
Authors: Kim Chul Jang , Nishi Kayoko , Isono Takahiro , Okuyama Yusuke , Tambe Yukihiro , Okada Yusaku , Inoue Hirokazu 
Published: May 2009

AbstractAlternative splicing in the cyclin D1 gene produces cyclin D1b variant which lacks a C‐terminal region containing the threonine‐286 (T286) phosphorylation site required for nuclear export. We have shown that the expression of the cyclin D1b variant is detected in about 60% of human bladder cancer tissues (15/26) and cell lines (3/5). To examine the role of the cyclin D1b variant in bladder carcinogenesis, we introduced wild‐type cyclin D1a, cyclin D1b variant or mutant cyclin D1‐T286A cDNAs into a human bladder cancer cell line, SBT991, in which cyclin D1b transcript was not expressed, and compared their oncogenic activities. Ectopic expression of cyclin D1b promoted cell invasiveness and anchorage‐independent growth of the cancer cells. On the other hand, cyclin D1‐T286A enhanced anchorage‐independent growth, but did not promote cell invasiveness. The amount of nuclear‐localized cyclin D1b and cyclin D1‐T286A was higher than that of nuclear‐localized cyclin D1a. In addition, introduction of siRNA specific for cyclin D1b into cells of the T24 bladder cancer cell line, in which cyclin D1b transcript was expressed, significantly suppressed cell invasiveness. Immunoprecipitation analysis revealed that cyclin D1a and cyclin D1‐T286A could bind to cyclin‐dependent kinase 4 (CDK4) but cyclin D1b has lost its capacity to associate with CDK4. Unlike cyclin D1a and cyclin D1‐T286A, expression of cyclin D1b did not enhance phosphorylation of Rb protein in SBT991 cells. These results indicate that cyclin D1b promotes cell invasiveness independent of binding to CDK4 to enhance Rb phosphorylation. Mol. Carcinog. © 2009 Wiley‐Liss, Inc.
Urothelial overexpression of insulin‐like growth factor‐1 increases susceptibility to p‐cresidine‐induced bladder carcinogenesis in transgenic mice
Authors: Hursting Stephen D. , Perkins Susan N. , Lavigne Jackie A. , Beltran Linda , Haines Diana C. , Hill Heather L. , Alvord W. Gregory , Barrett J. Carl , DiGiovanni John 
Published: May 2009

AbstractTo establish a role for insulin‐like growth factor‐1 (IGF‐1) in bladder cancer susceptibility, we tested the effect of p‐cresidine, a potent bladder carcinogen, in transgenic (TG) mice with human IGF‐1 expression in the bladder driven by the bovine keratin 5 promoter (referred to as BK5.IGF‐1 TG mice). Indomethacin was also tested to determine if the cyclooxygenase (COX) pathway is a target for bladder cancer prevention in this model. Thirty‐three female BK5.IGF‐1 TG mice and 29 female nontransgenic littermates were randomized to the following treatments: (1) AIN‐76A diet; (2) AIN‐76A diet with 0.5% p‐cresidine; or (3) AIN‐76A diet with 0.5% p‐cresidine + 0.00075% indomethacin. BK5.IGF‐1 TG mice, with twofold greater total serum IGF‐1 than nontransgenic mice, exhibited greatly increased susceptibility to p‐cresidine‐induced bladder tumors compared to nontransgenic mice. The most common type of bladder tumor in the BK5.IGF‐1 TG mice was transitional cell carcinoma, which is the predominant type of bladder cancer observed in developed countries. Indomethacin inhibition of bladder tumor development in BK5.IGF‐1 TG mice was not statistically significant. These results present further evidence for the role of IGF‐1 in bladder cancer progression. In addition, these transgenic mice provide a useful model for studying the role of the IGF‐1 pathway in bladder carcinogenesis and its prevention. © 2009 Wiley‐Liss, Inc.
Bioaccessibility, biotransformation, and transport of α‐mangostin from Garcinia mangostana (Mangosteen) using simulated digestion and Caco‐2 human intestinal cells
Authors: Bumrungpert Akkarach , Kalpravidh Ruchaneekorn W. , Suksamrarn Sunit , Chaivisuthangkura Apinya , Chitchumroonchokchai Chureeporn , Failla Mark L. 
Published: May 2009

Abstractα‐ and γ‐Mangostin are the most abundant prenylated xanthones present in the fruit of the mangosteen tree. These compounds have been reported to possess numerous bioactivities that have provided the impetus for use of mangosteen products as nutraceuticals and in functional foods and dietary supplements. The health‐promoting benefits of mangosteen are dependent on delivery of the xanthones to target tissues. Here, we used simulated digestion and Caco‐2 cells to investigate the digestive stability, bioaccessibility, and intestinal cell transport of α‐ and γ‐ mangostin. Recovery of α‐ and γ‐mangostin after simulated digestion of pericarp and fruit pulp exceeded 90%. Transfer of α‐ and γ‐mangostin to the aqueous fraction during simulated digestion was efficient (65–74%) and dependent on bile salts suggesting that micellarization is required for optimal bioaccessibility of xanthones. Cell uptake of xanthones from micelles was dose dependent and intracellular concentrations were maximum by 1 h. Both free and phase II metabolites of α‐mangostin were transported in the basolateral compartment and metabolites also effluxed into the apical chamber. Transepithelial transport of α‐mangostin was increased during prandial‐like compared to fasted conditions suggesting that absorption is enhanced by dietary fat.
Yoghurt impacts on the excretion of phenolic acids derived from colonic breakdown of orange juice flavanones in humans
Authors: Roowi Suri , Mullen William , Edwards Christine A. , Crozier Alan 
Published: May 2009

AbstractHuman urine was collected over a 24 h period after the consumption of 250 mL of (i) water, (ii) orange juice, and (iii) orange juice plus 150 mL of full fat natural yoghurt. The orange juice contained 168 μmol of hesperetin‐7‐O‐rutinoside and 18 μmol of naringenin‐7‐O‐rutinoside. GC‐MS analysis of the urine identified nine phenolic acids, five of which, 3‐hydroxyphenylacetic acid, 3‐hydroxyphenylhydracrylic acid, dihydroferulic acid, 3‐methoxy‐4‐hydroxyphenylhydracrylic acid and 3‐hydroxyhippuric acid, were associated with orange juice consumption indicating that they were derived from colonic catabolism of hesperetin‐7‐O‐rutinoside. The overall 0–24 h excretion of the five phenolic acids was 6.7 ± 1.8 μmol after drinking water and this increased significantly (p < 0.05) to 62 ± 18 μmol, equivalent to 37% of the ingested flavanones, following orange juice consumption. When the orange juice was ingested with yoghurt excretion fell back markedly to 9.3 ± 4.4 μmol. This was not due to a difference in mouth to caecum transit time, as measured with breath hydrogen production, though possibly there may have been a slowing of the bulk of the meal reaching the large intestine which may then have altered the catabolism of the flavanones to phenolic acids by the colonic microbiota.
Bilberry and its main constituents have neuroprotective effects against retinal neuronal damagein vitroandin vivo
Authors: Matsunaga Nozomu , Imai Shunsuke , Inokuchi Yuta , Shimazawa Masamitsu , Yokota Shigeru , Araki Yoko , Hara Hideaki 
Published: May 2009

AbstractOur aim was to determine whether aVaccinium myrtillus(bilberry) anthocyanoside (VMA) and/or its main anthocyanidin constituents (cyanidin, delphinidin, and malvidin) can protect retinal ganglion cells (RGCs) against retinal damagein vitroandin vivo. In RGC cultures (RGC‐5, a rat ganglion cell‐line transformed using E1A virus)in vitro, cell damage and radical activation were induced by 3‐(4‐morpholinyl) sydnonimine hydrochloride (SIN‐1, a peroxynitrite donor). Cell viability was measured using a water‐soluble tetrazolium salt assay. Intracellular radical activation within RGC‐5 cells was evaluated using 5‐(and‐6)‐chloromethyl‐2,7‐dichlorodihydrofluorescein diacetate acetyl ester (CM‐H2DCFDA). Lipid peroxidation was assessed using the supernatant fraction of mouse forebrain homogenates. In micein vivo, we evaluated the effects of VMA onN‐methyl‐D‐aspartic acid (NMDA)‐induced retinal damage using hematoxylin‐eosin and terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling (TUNEL) stainings. VMA and all three anthocyanidins (i) significantly inhibited SIN‐1‐induced neurotoxicity and radical activation in RGC‐5, (ii) concentration‐dependently inhibited lipid peroxidation in mouse forebrain homogenates. Intravitreously injected VMA significantly inhibited the NMDA‐induced morphological retinal damage and increase in TUNEL‐positive cells in the ganglion cell layer. Thus, VMA and its anthocyanidins have neuroprotective effects (exerted at least in partviaan anti‐oxidation mechanism) in thesein vitroandin vivomodels of retinal diseases.
Synthesis and properties of photo‐functional hyperbranched polymers
Authors: Nishikubo Tadatomi , Kudo Hiroto , Maruyama Ken 
Published: May 2009

AbstractSince dendritic polymers have excellent characteristic physical properties such as low viscosity, good solubility, and many functional groups, compared with corresponding linear polymers, they have been of great interest in the field of polymer chemistry. Among them, hyperbranched polymers have great possibility as high performance and new photo‐curable polymers and oligomers in chemical industry. In this paper, we report the synthesis and photochemical reaction of certain hyperbranched polymers such as hyperbranched epoxy‐methacrylate, hyperbranched oxetane‐methacrylate, and hyperbranched polyurethane‐methacrylate, and some physical properties of the resulting cured films. Copyright © 2009 John Wiley & Sons, Ltd.
Microfluidic chip fabrication using hot embossing and thermal bonding of COP
Authors: Pemg Boe‐Yu , Wu Chih‐Wei , Shen Yung‐Kang , Lin Yi 
Published: May 2009

AbstractThe application of silicon mold inserts by micro‐hot embossing molding has been explored in microfluidic chip fabrication. For the mold insert, this study employed an SU‐8 photoresist to coat the silicon wafer. Ultraviolet light was then used to expose the pattern on the SU‐8 photoresist surface. This study replicates the microstructure of the silicon mold insert by micro‐hot embossing molding. Different processing parameters (embossing temperature, embossing pressure, embossing time, and de‐molding temperature) for the cycle‐olefin polymer (COP) film of microfluidic chips are evaluated. The results showed that the most important parameter for replication of molded microfluidic chip is embossing temperature. De‐molding temperature is the most important parameter for surface roughness of the molded microfluidic chip. The microchannel is bonded with a cover by thermal bonding processing to form the sealed microfluidic chip. The bonding temperature is the most important factor in the bonding strength of the sealed microfluidic chip. Copyright © 2009 John Wiley & Sons, Ltd.
Testicular stem cells for fertility preservation: Preclinical studies on male germ cell transplantation and testicular grafting
Authors: Schlatt Stefan , Ehmcke Jens , Jahnukainen Kirsi 
Published: May 2009

AbstractSpermatogonial stem cells open novel strategies for preservation of testicular tissue and fertility preservation in boys and men exposed to gonadotoxic therapies. This review provides an update on the physiology of spermatogonial stem cells in rodent and primate testes. Species‐specific differences must be considered when new technologies on testicular stem cells are considered. Germ cell transplantation is presented as one novel and promising strategy. Whereas this technique has become an important research tool in rodents, a clinical application must still be regarded as experimental and many aspects of the procedure need to be optimized prior to a safe and efficient clinical application in men. Testicular grafting opens another exciting strategy for fertility preservation. Autologous and xenologous transfer of immature tissue revealed a high regenerative potential of immature testicular tissue. Grafting was applied in rodents and primates and resulted in the generation of sperm. Further research is needed before an application in humans can be considered safe and efficient. Despite the current limitations in regard to the generation of sperm from cryopreserved male germline cells and tissues, protocols for cryopreservation of testicular tissue are available and reveal a promising outcome. Since future improvements of germ cell transplantation and grafting approaches can be assumed, bioptic retrieval and cryopreservation of testicular tissue fragments should be performed in oncological patients at high risk of fertility loss since this is their only option to maintain their fertility potential. Pediatr Blood Cancer 2009;53:274–280. © 2009 Wiley‐Liss, Inc.
Off‐label use of rFVIIa in children with excessive bleeding: A consecutive study of 153 off‐label uses in 139 children
Authors: Young Guy , Wicklund Brian , Neff Philip , Johnson Chelsea , Nugent Diane J. 
Published: May 2009

AbstractBackgroundRecombinant factor VIIa is a general hemostatic agent. Randomized trials have demonstrated effectiveness in adults; however, data in children are confined to case reports and series subject to publication bias.ProceduresA consecutive cohort of children treated with rFVIIa was identified via inspection of pharmacy records. Data collected included demographic data, underlying disorders, reason for rFVIIa use, dosing, thrombotic events, and mortality. Efficacy was scored subjectively on a 3‐point scale (complete, partial, or no response) and supported by a measurement of transfusion of red cells, platelets, fresh frozen plasma, and cryoprecipitate in the 3 days prior to and following use of rFVIIa. Patients with hemophilia and congenital factor VII deficiency were excluded.ResultsSixty‐five percent had a complete response and 26% had a partial response. Following treatment, there was a mean reduction in transfusions of 14.2 ml/kg for red blood cells, 10.9 ml/kg for platelets, 9 ml/kg for fresh frozen plasma, and 4.6 ml/kg for cryoprecipitate. Thrombosis occurred in 4.3% of patients with the highest rate being in neonates (17.6%).ConclusionsrFVIIa is an effective general hemostatic agent for management of excessive bleeding in children and that with the exception of neonates has excellent safety. Pediatr Blood Cancer 2009;53:179–183. © 2009 Wiley‐Liss, Inc.
Splenic infarction and subsequent splenic rupture in a patient with paroxysmal nocturnal hemoglobinuria and heparin‐induced thrombocytopenia
Authors: Magnan Heather , Kayton Mark L. , DiMichele Donna M. , Araten David J. , Kernan Nancy A. , Boulad Farid 
Published: May 2009

AbstractWe describe a patient with paroxysmal nocturnal hemoglobinuria (PNH) and no previous history of thrombosis who presented with hepatic venous thromboses and subsequently developed splenic infarction and rupture requiring splenectomy while on anticoagulation therapy for the hepatic thromboses. The patient's anticoagulation was complicated by heparin‐induced thrombocytopenia (HIT) highlighting the unique management challenge presented by PNH in combination with HIT. Pediatr Blood Cancer 2009;53:472–474. © 2009 Wiley‐Liss, Inc.
Dyskeratosis congenita: The first NIH clinical research workshop
Authors: Savage Sharon A. , Dokal Inderjeet , Armanios Mary , Aubert Geraldine , Cowen Edward W. , Domingo Demetrio L. , Giri Neelam , Greene Mark H. , Orchard Paul J. , Tolar Jakub , Tsilou Ekaterini , Van Waes Carter , Wong Judy M.Y. , Young Neal S. , Alter Blanche P. 
Published: May 2009

AbstractDyskeratosis congenita (DC) is a heterogeneous inherited bone marrow failure syndrome, characterized by abnormally short telomeres and mutations in telomere biology genes. The spectrum of telomere biology disorders is growing and the clinical management of these patients is complex. A DC‐specific workshop was held at the NIH on September 19, 2008; participants included physicians, patients with DC, their family members, and representatives from other support groups. Data from the UK's DC Registry and the NCI's DC cohort were described. Updates on the function of the known DC genes were presented. Clinical aspects discussed included androgen therapy, stem cell transplant, cancer risk, and cancer screening. Families with DC met for the first time and formed a family support group (http://www.dcoutreach.com/). Ongoing, open collaboration between the clinical, scientific, and family communities is required for continued improvement in our understanding of DC and the clinical consequences of telomeric defects. Pediatr Blood Cancer 2009;53:520–523. © 2009 Wiley‐Liss, Inc.
Monotherapy with thalidomide for treatment of spinal cord hemangioblastomas in a patient with von Hippel–Lindau disease
Authors: Sardi Iacopo , Sanzo Massimiliano , Giordano Flavio , Buccoliero Anna Maria , Mussa Federico , Aricò Maurizio , Genitori Lorenzo 
Published: May 2009

AbstractVon Hippel–Lindau (VHL) disease is a cancer‐prone syndrome characterized by abnormalities in vascular proliferation and the development of both the visceral and CNS tumors. Complications from hemangioblastoma are among the principal causes of death from this syndrome. Antiangiogenic therapy has been used with different modalities in patients suffering from such complications. Here, we describe an adolescent with VHL complicated by progressive, multifocal spinal hemangioblastomas. Treatment with single‐agent thalidomide over the course of 3 years was associated with an unexpected stabilization of the disease. The antiangiogenic effect of thalidomide may be associated with the control of progressive hemangioblastoma. Pediatr Blood Cancer 2009;53:464–467. © 2009 Wiley‐Liss, Inc.
Sample preparation issues for tissue imaging by imaging MS
Authors: Kaletaş Başak Kükrer , van der Wiel Ingrid M. , Stauber Jonathan , Lennard J. Dekker  , Güzel Coşkun , Kros Johan M. , Luider Theo M. , Heeren Ron M. A. 
Published: May 2009

AbstractImaging MS is a powerful technique that combines the chemical and spatial analysis of surfaces. It allows spatial localization of multiple different compounds that are recorded in parallel without the need of a label. It is currently one of the rapidly developing techniques in the proteomics toolbox. Different complementary imaging MS methods, i.e. MALDI and secondary ion MS imaging for direct tissue analysis, can be applied on exactly the same tissue sample. This allows the identification of small molecules, peptides and proteins present on the same sample surface. Sample preparation is crucial to obtain high quality, reliable and reproducible complementary molecular images. It is essential to optimize the conditions for each step in the sample preparation protocol, ranging from sample collection and storage to surface modification. In this article, we review and discuss the importance of correct sample treatment in case of MALDI and secondary ion MS imaging experiments and describe the experimental requirements for optimal sample preparation.
Phosphoproteomic analysis of distinct tumor cell lines in response to nocodazole treatment
Authors: Nagano Kohji , Shinkawa Takashi , Mutoh Hironori , Kondoh Osamu , Morimoto Sayuri , Inomata Noriyuki , Ashihara Motooki , Ishii Nobuya , Aoki Yuko , Haramura Masayuki 
Published: May 2009

AbstractHere, we report for the first time a comparative phosphoproteomic analysis of distinct tumor cell lines in the presence or absence of the microtubule‐interfering agent nocodazole. In total, 1525 phosphorylation sites assigned to 726 phosphoproteins were identified using LC‐MS‐based technology following phosphopeptide enrichment. Analysis of the amino acid composition surrounding the identified in vivo phosphorylation sites revealed that they could be classified into two motif groups: pSer‐Pro and pSer‐Asp/Glu. Phosphoproteomic change resulting from nocodazole treatment varied among cell lines in terms of the numbers of total phosphopeptides identified, motif groups, and functional annotation groups; however, the cell lines were equally sensitive to nocodazole. The identified phosphoproteome subset contained major signaling proteins and proteins known to be involved in mitosis, but did not always exhibit the same changes in the tumor cells from nocodazole treatment. In spite of the complex changes observed in the phosphorylation of many of the proteins, possible common features induced by nocodazole were found, including phosphorylation of nucleophosmin (NPM) S254 and coatomer protein complex, subunit α (COPA) S173, suggesting that the events are not cell‐type specific but events generally occurring in mitosis or induced by a microtubule‐interfering agent. Further, temporal analysis of phosphoproteome change revealed that phosphorylation of NPM S254 and COPA S173 was observed from the early (6 h) and late (24 h) time point after nocodazole treatment, respectively, suggesting that NPM S254 may be involved in the induction of M‐phase arrest by nocodazole, whereas COPA S173 may be caused as a result of M‐phase arrest.