bluuebunny/crossref_metadata_2025_split huggingface dataset:
Colorectal cancers show distinct mutation spectra in members of the canonical WNT signaling pathway according to their anatomical location and type of genetic instability
Authors: Albuquerque Cristina , Baltazar Célia , Filipe Bruno , Penha Filipa , Pereira Teresa , Smits Ron , Cravo Marília , Lage Pedro , Fidalgo Paulo , Claro Isabel , Rodrigues Paula , Veiga Isabel , Ramos José Silva , Fonseca Isabel , Leitão Carlos Nobre , Fodde Riccardo 
Published: May 2010

AbstractIt is unclear whether the mutation spectra in WNT genes vary among distinct types of colorectal tumors. We have analyzed mutations in specific WNT genes in a cohort of 52 colorectal tumors and performed a meta‐analysis of previous studies. Notably, significant differences were found among the mutation spectra. We have previously shown that in familial adenomatous polyposis, APC somatic mutations are selected to provide the “just‐right” level of WNT signaling for tumor formation. Here, we found that APC mutations encompassing at least two β‐catenin down‐regulating motifs (20 a.a. repeats) are significantly more frequent in microsatellite unstable (MSI‐H) than in microsatellite stable (MSS) tumors where truncations retaining less than two repeats are more frequent (P = 0.0009). Moreover, in cases where both APC hits are detected, selection for mutations retaining a cumulative number of two 20 a.a. repeats became apparent in MSI‐H tumors (P = 0.001). This type of mutations were also more frequent in proximal versus distal colonic tumors, regardless of MSI status (P = 0.0008). Among MSI‐H tumors, CTNNB1 mutations were significantly more frequent in HNPCC than in sporadic lesions (28% versus 6%, P < 10‐6) and were preferentially detected in the proximal colon, independently of MSI status (P = 0.017). In conclusion, the observed spectra of WNT gene mutations in colorectal tumors are likely the result from selection of specific levels of β‐catenin signaling, optimal for tumor formation in the context of specific anatomical locations and forms of genetic instability. We suggest that this may underlie the preferential location of MMR deficient tumors in the proximal colon. © 2010 Wiley‐Liss, Inc.
The heart of the South American rattlesnake, Crotalus durissus
Authors: Jensen Bjarke , Abe Augusto S. , Andrade Denis V. , Nyengaard Jens R. , Wang Tobias 
Published: May 2010

AbstractMost anatomical and physiological studies of the sauropsid heart have focused on species with extraordinary physiologies, and detailed anatomical descriptions of hearts from sauropsids with more common physiologies are therefore warranted. Here, we present a comprehensive study of the cardiac anatomy of the South American rattlesnake (Crotalus durissus). The cardiovascular physiology of this species has been investigated in a number of studies, whereas only a few cursory studies exist on the cardiac anatomy of viperid snakes. The heart of C. durissus is typically squamate in many regards. Both atria are thin‐walled sacs, and the right atrium is the most voluminous. The single ventricle contains three major septa; the vertical septum, the muscular ridge (MR), and the bulbuslamelle. These partially divide the ventricle into three chambers; the systemic and left‐sided cavum arteriosum (CA), the pulmonary and right‐sided cavum pulmonale, and the medial cavum venosum (CV). The MR is the most developed septum, and several additional and minor septa are found within the CA and CV. An extraordinary thin cortical layer encloses the ventricle, and it is irrigated by a remarkably rich arborization of coronary arteries. Previous studies show high degrees of blood flow separation in the Crotalus heart, and this can only be explained by the coordinated actions of the septa and the prominent atrioventricular valves. J. Morphol. 271:1066‐1077, 2010. © 2010 Wiley‐Liss, Inc.
Goblin spiders without distinct receptacula seminis (Arachnida: Araneae: Oonopidae)
Authors: Burger Matthias 
Published: May 2010

AbstractThe mechanism of sperm transfer in spiders is unique among arthropods. Males use their modified pedipalps to transmit sperm which is received by females of most spider groups in specialized organs, the receptacula seminis. Oonopidae are a diverse spider family belonging to the Haplogynae. Female haplogynes have supposedly simple genitalia where the receptacula derive from the uterus externus, which is the duct passed by the eggs. The purpose of the present study is to describe the unusual female genitalia of the oonopids Xyphinus sp. and Ischnothyreus sp. by combining three methods of investigation: histological serial sections, scanning electron microscopy, and X‐ray ultra microscopy, and to compare the results with previous findings on haplogynes. Furthermore, the male palps are briefly described. The female genitalia of both investigated species do not correspond to the general description of haplogyne genitalia given in the literature. Distinct receptacula are lacking in both species. In Xyphinus sp., sperm are deposited into the uterus internus. A sclerite with attached muscles in the uterus wall might serve females to lock the uterus during copulation in order to prevent sperm from getting into it. The male palp of Xyphinus sp. bears complicated structures indicating that males could use the palps as copulatory courtship devices. The ventral scutum of female Ischnothyreus sp. forms a large depression which was always empty in the examined specimens. Sperm could be stored in a large fold of the uterus externus. As in Xyphinus sp., spermatozoa inside the uterus internus of Ischnothyreus sp. indicate that fertilization takes place in the uterus or the ovary. The function of a sclerotized squiggled tube in females of Ischnothyreus sp. remains unknown. Surrounding glandular tissue suggests the involvement of the tube in glandular activity. The male palp shows a simple embolus accompanied by a small conductor. J. Morphol. 271:1110–1118, 2010. © 2010 Wiley‐Liss, Inc.
A comparison of cyclic variations in anterior knee laxity, genu recurvatum, and general joint laxity across the menstrual cycle
Authors: Shultz Sandra J. , Levine Beverly J. , Nguyen Anh‐Dung , Kim Hyunsoo , Montgomery Melissa M. , Perrin David H. 
Published: May 2010

AbstractChanges in anterior knee laxity (AKL), genu recurvatum (GR) and general joint laxity (GJL) were quantified across days of the early follicular and early luteal phases of the menstrual cycle in 66 females, and the similarity in their pattern of cyclic variations examined. Laxity was measured on each of the first 6 days of menses (M1–M6) and the first 8 days following ovulation (L1–L8) over two cycles. The largest mean differences were observed between L5 and L8 for AKL (0.32 mm), and between L5 and M1 for GR (0.56°) and GJL (0.26) (p < 0.013). At the individual level, mean absolute cyclic changes in AKL (1.8 ± 0.7 mm, 1.6 ± 0.7 mm), GR (2.8 ± 1.0°, 2.4 ± 1.0°), and GJL (1.1 ± 1.1, 0.7 ± 1.0) were more apparent, with minimum, maximum and delta values being quite consistent from month to month (ICC2,3 = 0.51–0.98). Although the average daily pattern of change in laxity was quite similar between variables (Spearman correlation range 0.61 and 0.90), correlations between laxity measures at the individual level were much lower (range −0.07 to 0.43). Substantial, similar, and reproducible cyclic changes in AKL, GR, and GJL were observed across the menstrual cycle, with the magnitude and pattern of cyclic changes varying considerably among females. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1411–1417, 2010
Polyneuropathy caused by cobalt–chromium metallosis after total hip replacement
Authors: Ikeda Tokuhei , Takahashi Kazuya , Kabata Tamon , Sakagoshi Daigo , Tomita Katsuro , Yamada Masahito 
Published: May 2010

AbstractAlthough metal intoxication after arthroplasty causes various symptoms, polyneuropathy has never been the focus of clinical investigation. We report the case of a 56‐year‐old woman with metal neuropathy. She had metallosis after hip arthroplasty with a cobalt–chromium alloy prosthesis. She developed progressive sensory disturbance, hearing loss, and hypothyroidism. Sural nerve biopsy indicated axonopathy. After exchange arthroplasty, blood levels of cobalt and chromium decreased, and her symptoms improved. Cobalt or chromium can cause axonopathy. Muscle Nerve, 2010
Psychosocial adjustment during the transition to parenthood of Portuguese couples who conceived spontaneously or through assisted reproductive technologies
Authors: Gameiro Sofia , Moura‐Ramos Mariana , Canavarro Maria Cristina , Soares Isabel 
Published: May 2010

AbstractWe examined the psychosocial adjustment of 35 Portuguese couples who conceived through Assisted Reproductive Technologies (ART) and 31 couples with a spontaneous conception during their transition to parenthood (pregnancy and 4 months postpartum). Couples completed self‐report questionnaires regarding their perceptions of pregnancy and parenthood, psychological distress, quality of life, marital relationship, and parenting stress. Compared with parents who conceived spontaneously, parents who conceived through ART perceived pregnancy as being more risky and demanding, reported a decrease in their psychological quality of life, and ART fathers only perceived themselves as being more competent than fathers who conceived spontaneously. Healthcare professionals should be aware of need of couples in their efforts to adapt to the individual and relational challenges associated with the transition to parenthood. © 2010 Wiley Periodicals, Inc. Res Nurs Health 33:207–220, 2010
A modified hermeneutic phenomenological approach toward individuals who have autism
Authors: Newman Claire , Cashin Andrew , Waters Cheryl D. 
Published: May 2010

AbstractIndividuals with autism have a unique cognitive processing style characterized by impaired abstraction, impaired theory of mind, and visual as opposed to linguistic processing of information. A consequence of this unique cognitive processing style is that traditional ways of hermeneutical phenomenological examination may be inadequate to achieve the kind of understanding of experience toward which this method is directed. In order to stay true to Heidegger's hermeneutic phenomenology, we needed to develop modifications to this research methodology, which include the use of visual aids to promote participant engagement and access the eidetic memory of a participant with autism, so as to elicit concrete descriptors of an experience. © 2010 Wiley Periodicals, Inc. Res Nurs Health 33:265–271, 2010
An improvement on the adhesion‐strength of laminated ultra‐high‐molecular‐weight polyethylene fabrics: surface‐etching/modification using highly effective helium/oxygen/nitrogen plasma treatment
Authors: Yeh Jen‐Taut , Lai Yu‐Ching , Suen Maw‐Cherng , Chen Cheng‐Chi 
Published: May 2010

AbstractIn this study, helium/oxygen/nitrogen (He/O2/N2)‐plasma was used to etch/modify the surface of ultra‐high‐molecular‐weight polyethylene (UHMWPE) fiber. After coated with polyurethane (PU), the plasma treated UHMWPE fabrics were laminated. It was found that the values of peeling strength between the laminated UHMWPE fabrics treated with He/O2/N2‐plasma were significantly higher (3–4 times) than that between pristine fabrics. The hydrophilic property and the value of the surface roughness of the UHMWPE fibers increased significantly after treated with He/O2/N2‐plasma. The mechanism of the oxidation/degradation of the polymers on the surface of the UHMWPE fiber during He/O2/N2‐plasma treatment was suggested. In addition, it was found that the higher content of functional groups (carbonyl, aldehyde, and carboxylic acid) on fiber surface and the higher value of surface roughness of the UHMWPE fiber treated with He/O2/N2‐plasma could significantly improve the adhesion‐strength of the laminated UHMWPE fabric. Especially, the micro‐aperture on the surface of UHMWPE fiber caused by the strenuous etching of He/O2/N2‐plasma treatment was also an important factor on improving the adhesion‐strength between the laminated UHMWPE fabrics. Copyright © 2010 John Wiley & Sons, Ltd.
DeepQuanTR: MALDI‐MS‐based label‐free quantification of proteins in complex biological samples
Authors: Fugmann Tim , Neri Dario , Roesli Christoph 
Published: May 2010

AbstractThe quantification of changes in protein abundance in complex biological specimens is essential for proteomic studies in basic and applied research. Here we report on the development and validation of the DeepQuanTR software for identification and quantification of differentially expressed proteins using LC‐MALDI‐MS. Following enzymatic digestion, HPLC peptide separation and normalization of MALDI‐MS signal intensities to the ones of internal standards, the software extracts peptide features, adjusts differences in HPLC retention times and performs a relative quantification of features. The annotation of multiple peptides to the corresponding parent protein allows the definition of a Protein Quant Value, which is related to protein abundance and which allows inter‐sample comparisons. The performance of DeepQuanTR was evaluated by analyzing 24 samples deriving from human serum spiked with different amounts of four proteins and eight complex samples of vascular proteins, derived from surgically resected human kidneys with cancer following ex vivo perfusion with a reactive ester biotin derivative. The identification and experimental validation of proteins, which were differentially regulated in cancerous lesions as compared with normal kidney, was used to demonstrate the power of DeepQuanTR. This software, which can easily be used with established proteomic methodologies, facilitates the relative quantification of proteins derived from a wide variety of different samples.
Comparative physiological, ultrastructural and proteomic analyses reveal sexual differences in the responses ofPopulus cathayanaunder drought stress
Authors: Zhang Sheng , Chen Fugui , Peng Shuming , Ma Wujun , Korpelainen Helena , Li Chunyang 
Published: May 2010

AbstractDrought is a major abiotic stress, limiting the survival and growth of young plants. However, little is known about sex‐dependent responses to drought at the proteome level. In this study, we carried out investigations on comparative proteomics, combined with physiological and organelle structure analyses, in males and females ofPopulus cathayanaRehd. Three‐month‐old poplar cuttings were treated at 30% of field capacity and at 100% field capacity as a control in a greenhouse for 40 days. Drought greatly inhibited plant growth, damaged the photosynthetic system and destructed the structures of chloroplasts, mitochondria and cellular membranes. However, males suffered less from the adverse effects of drought than did females. Using 2‐DE, 563 spots were detected, of which 64 spots displayed significant drought effect and 44 spots displayed a significant sex by drought interaction effect. The results suggest that the different responses to drought stress detected between the sexes have a close relationship to the changes in the expression of sex‐dependent proteins, including,e.g.photosynthesis‐related proteins, homeostasis‐related proteins and stress response proteins. These proteins could contribute to a physiological advantage under drought, giving potential clues for understanding sexual differences in the performance of plants in different environments.
MASPECTRAS 2: An integration and analysis platform for proteomic data
Authors: Mohien Ceereena Ubaida , Hartler Jürgen , Breitwieser Florian , Rix Uwe , Rix Lily Remsing , Winter Georg E. , Thallinger Gerhard G. , Bennett Keiryn L. , Superti‐Furga Giulio , Trajanoski Zlatko , Colinge Jacques 
Published: May 2010

AbstractMASPECTRAS 2 is a freely available platform for integrating MS protein identifications with information from the major bioinformatics databases (ontologies, domains, literature, etc.). It assists researchers in understanding their data and publishing through sample comparisons, targeted queries, summaries, and exports in multiple formats such as PRIDE XML (Jones et al., Nucleic Acids Res. 2008, 36, D878–D883). MASPECTRAS 2 also comprises mechanisms to facilitate its integration in a high‐throughput infrastructure. We illustrate application of MASPECTRAS 2 with unpublished tyrosine kinase inhibitor drug proteomics profiles in cancerous cells.
Quantitative evaluation of tryptophan oxidation in actin and troponin I from skeletal muscles using a rat model of acute oxidative stress
Authors: Fedorova Maria , Todorovsky Toni , Kuleva Nadezda , Hoffmann Ralf 
Published: May 2010

AbstractIncreased levels of “ROS” cause oxidative stress and are believed to play a key role in the development of age‐related diseases and mammalian aging, e.g. through the oxidation of residues, at or close to, the protein surface. In this study, we have investigated the effects of ROS on tryptophan residues in alpha skeletal actin and troponin I (fast skeletal muscle isoform) using an established rat model of acute oxidative stress induced by X‐ray irradiation. In the control samples (no oxidative stress), the single Trp residue of troponin I (position 161) and the four tryptophan residues present in actin (positions 79, 86, 340, and 356) were only oxidized at very low levels. Post‐irradiation, the level of oxidized versions increased for most positions within 3 h. Tryptophan residues located inside the proteins, however, required longer time periods. Based on the increment masses of the tryptophan positions calculated from the b‐ and y‐ion series of the tandem mass spectra, the following oxidation products of tryptophan were detected: kynurenine; oxolactone; hydroxytryptophan or oxindolylalanine (isobaric); hydroxykynurenine; dioxindolylalanine, N‐formylkynurenine or dihydroxytryptophan (all three isobaric); and hydroxyl‐N‐formylkynurenine, with mass gains relative to tryptophan of 4, 14, 16, 20, 32, and 48 u, respectively. Despite a partial recovery after 24 h, the degree of oxidation of all oxidized versions was still higher than in the control samples.
Consideration of regional difference in design and analysis of multi‐regional trials
Authors: Hung H. M. James , Wang Sue‐Jane , O'Neill Robert T. 
Published: May 2010

AbstractClinical trial strategy, particularly in developing pharmaceutical products, has recently expanded to a global level in the sense that multiple geographical regions participate in the trial simultaneously under the same study protocol. The possible benefits of this strategy are obvious, at least from the cost and efficiency considerations. The challenges with this strategy are many, ranging from trial or data quality assurance to statistical methods for design and analysis of such trials. In many regulatory submissions, the presence of regional differences in the estimated treatment effect, whether they are different only in magnitude or in direction, often presents great difficulty in interpretation of the global trial results, particularly for the acceptability by the local regulatory authorities. This article presents a number of useful statistical analysis tools for exploration of regional differences and a method that may be worth consideration in designing a multi‐regional clinical trial. Published in 2010 by John Wiley & Sons, Ltd.
The effect of damming on biological quality according to macroinvertebrates in some Estonian streams, Central—Baltic Europe: A pilot study
Authors: Käiro Kairi , Möls Tõnu , Timm Henn , Virro Taavi , Järvekülg Rein 
Published: May 2010

AbstractThe effect of damming on the structure of the macroinvertebrate community and biological quality was studied in nine (the 3–6th order) lowland streams of Estonia, Central—Baltic ecoregion of Europe. Four habitats—reservoirs with accumulated fine sediments, reservoirs with hard bottom, and two corresponding below‐dam areas (both fast‐flowing)—were compared to study whether and how significantly the bottom substrata in dammed areas affected macroinvertebrates and biological quality downstream of dams. The standard kick‐net samples (1.25 m2, complemented with qualitative sample) were collected in autumn 2005—spring 2006. The multimetric biological quality, based on five macroinvertebrate indices (total taxa richness, EPT taxa richness, Average Score Per Taxon, Danish Stream Fauna Index, Shannon diversity) was estimated and compared with reference values. Biological quality in reservoirs with hard bottom and their downstream reaches corresponded to good, or even high quality. Conversely, damming affected biological quality significantly and negatively, above the dam if fine sediments were accumulated. The effect was the strongest within muddy reservoirs themselves (revealing moderate quality only). However, some harmful consequences of mud were observed also downstream of dams. The results also demonstrated that the indices of estimation of organic pollution and/or general quality were able to reflect significant changes in stream flow. Copyright © 2010 John Wiley & Sons, Ltd.
Cross‐plane information sharing for QoS in satellite–terrestrial integrated packet networks
Authors: Gopal Rajeev , Gopal Rohit 
Published: May 2010

AbstractThis paper introduces a standards‐based Quality‐of‐Service (QoS) architecture for satellite and terrestrial IP packet network integration to facilitate the evolution of the next generation integrated networks. Cross‐plane information sharing can extend the reach of cross‐layer improvements, typically applied for enhancing throughput and robustness in the wireless networks. Besides supporting real‐time interactive applications for all users, such a QoS architecture can also provide a prioritization framework for enhanced service assurance. The satellite packet transport operates in a spectral, power, and hardware resource constrained environment. It needs specific functions for packet classification, scheduling, and propagation, all operating within the broader context of dynamic resource allocations at the media access layer for transporting individual packets and their aggregates. Additional information, made available to these networking functions across the data, control, and management planes can facilitate key decision making at the individual packet flow level with the use of formal connection admission control (CAC). Our QoS scheme links the data and management planes with the use of IP differentiated services (Diffserv) information in the packet headers. Originally designed for packet traffic aggregates, Diffserv can be leveraged to provide QoS for the individual packet flows at specific data rates and packet drop sensitivity. This QoS architecture has been validated with a pioneering regenerative packet processing satellite system and it is also suitable for other networks that support flow‐level CAC, operating under dynamic network capacity management. Copyright © 2010 John Wiley & Sons, Ltd.
The effect of Ca co‐substitution in (Y1−xCax)Ba2 (Cu0.98Co0.02)3O7−δ (0 ≤ x ≤ 0.35) ceramic
Authors: Boudjema E. H. , Mahtali M. , Chamekh S. , Taoufik A. 
Published: May 2010

AbstractYBa2Cu3O7−δ cuprate is a suitable system for the study of impurity effect on superconducting properties because carrier concentration can be widely changed without introducing significant disorder. Thus, the effect of replacing Cu by Co atoms, and Y by Ca atoms is reported to provide useful information about the mechanism of high‐Tc superconductivity. In this paper, we have investigated the effect of Ca and Co simultaneous substitution on the structural and physical properties for a series of (Y1−xCax)Ba2(Cu0.98Co0.02)3O7−δ (0 ≤ x ≤ 0.35) ceramic. Doped Y123 samples are prepared by conventional solid‐state reaction method and characterized by X‐ray diffraction (XRD), SEM, and resistivity measurements. We found that the obtained crystalline structure is mainly orthorhombic. No parasite phase is observed. The orthorhombicity increases with Ca content. The analysis of the superconducting behavior of the samples showed that co‐substitution of Ca does not counteract the adverse influence of Co within the single‐phase range. Copyright © 2010 John Wiley & Sons, Ltd.