bluuebunny/crossref_metadata_2025_split huggingface dataset:
Curcumin enhances the anticancer effects of trichostatin a in breast cancer cells
Authors: Yan Guang , Graham Kimmer , Lanza‐Jacoby Susan 
Published: January 2012

AbstractBreast cancer patients with HER‐2 positive or estrogen receptor negative tumors have a poor prognosis because these tumors are aggressive and respond poorly to standard therapies. Histone deacetylase (HDAC) inhibitors have been shown to decreased cell survival, which suggests that HDAC inhibitors may be developed for preventing and treating breast cancer. Curcumin has anti‐inflammatory and proapoptotic effects in cancer cells. We determined whether the HDAC inhibitor, Tricostatin A (TSA) in combination with curcumin would produce greater antiproliferative and apoptotic effects than either agent alone. Increasing the concentration of curcumin from 10 to 20 µM enhanced the growth inhibitory effects of the combination in SkBr3 and 435eB breast cancer cells, which was accompanied by decreased viability along with decreased phosphorylation of ERK and Akt. The decreased cell viability observed in SkBr3 cells when curcumin was combined with TSA led to a G0/G1 cell cycle arrest and increased p21 and p27, and decreased Cyclin D1 protein expression. The combination induced cleavage of caspase 3 and poly(ADP‐ribose) polymerase‐1, suggesting that cell death occurred by apoptosis. There were no changes in protein expression of Bcl2, Bax, or Bcl‐xL and decreased expression of p53. The combination increased protein expression of phosphorylated JNK and phosphorylated p38. Pharmacological inhibition of JNK, but not p38, attenuated the decreased viability induced by the curcumin and TSA combination. We conclude that p53 independent apoptosis induced by combining curcumin and TSA involves JNK activation. These findings provide a rationale for exploring the potential benefits of the combination of curcumin with TSA for treatment of breast cancer. © 2012 Wiley Periodicals, Inc.
Rivastigmine as alternative treatment for refractory REM behavior disorder in Parkinson's disease
Authors: Di Giacopo Raffaella , Fasano Alfonso , Quaranta Davide , Marca Giacomo Della , Bove Francesco , Bentivoglio Anna Rita 
Published: January 2012

AbstractBackground:We report on a double‐blind, crossover pilot trial for the treatment of rapid eye movement behavior disorder (RBD) in 12 patients with Parkinson's disease in whom conventional therapy failed.Methods:We employed a patch of the cholinesterase inhibitor rivastigmine at a dose of 4.6 mg/24 hours for 3 weeks compared with placebo to reduce the frequency of RBD episodes. The number of RBD episodes was monitored by diaries of bed partners.Results:Rivastigmine was well tolerated in most patients, with minor side effects, mainly related to peripheral cholinergic action, and significantly reduced the mean frequency of RBD episodes during the observation time.Conclusions:The results of this pilot trial need to be confirmed by further studies on a larger number of patients. © 2012 Movement Disorder Society
Recommendations for myasthenia gravis clinical trials
Authors: Benatar Michael , Sanders Donald B. , Burns Ted M. , Cutter Gary R. , Guptill Jeffrey T. , Baggi Fulvio , Kaminski Henry J. , Mantegazza Renato , Meriggioli Matthew N. , Quan Joanne , Wolfe Gil I. ,   THE TASK FORCE ON MG STUDY DESIGN OF THE MEDICAL SCIENTIFIC ADVISORY BOARD OF THE MYASTHENIA GRAVIS FOUNDATION OF AMERICA
Published: January 2012

AbstractThe recommendations for clinical research standards published in 2000 by a task force of the Medical Scientific Advisory Board (MSAB) of the Myasthenia Gravis Foundation of America (MGFA) were largely successful in introducing greater uniformity in the recording and reporting of MG clinical trials. Recognizing that changes in clinical trial design and implementation may increase the likelihood that new therapies are developed for MG, the MGFA MSAB Task Force here presents updated recommendations for the design and implementation of clinical trials in MG, including (a) the use of a quantitative measure, such as the MG‐Composite, that is weighted for clinical significance and incorporates patient reported outcomes; (b) consideration of nontrial strategies; and (c) development of biomarkers that support mechanistic studies of pharmacotherapies. The hope is that these updated task force recommendations will expedite the development and acceptance of more effective and less noxious therapies for MG. Muscle Nerve 45: 909–917, 2012
Doxycycline ameliorates the dystrophic phenotype of skeletal and cardiac muscles in mdx mice
Authors: Pereira Juliano Alves , Taniguti Ana Paula Tiemi , Matsumura Cíntia , Marques Maria Julia , Neto Humberto Santo 
Published: January 2012

AbstractIntroduction: We examined whether doxycycline, an antibiotic member of the tetracycline family, improves the histopathology and muscle function in mdx mice, the experimental model of DMD. Methods: Doxycycline was administered for 36 days (starting on postnatal day 0) and for 9 months (starting at 8 months of age) in drinking water. Histopathological, biochemical (creatine kinase), and functional (forelimb muscle grip strength) parameters were evaluated in limb, diaphragm, and cardiac muscle. Results: Doxycycline significantly minimized the dystrophic phenotype of skeletal and cardiac muscles and improved forelimb muscle strength. The drug protected muscle fibers against myonecrosis and reduced inflammation. Furthermore, it slowed the progression of myocardial fibrosis. Conclusions: This study provides evidence that doxycycline may be a potential therapeutic agent for DMD. Muscle Nerve 46: 400–406, 2012
Analyses of soft‐state signaling protocols in GMPLS‐Based WDM networks
Authors: Arakawa Shin'ichi , Ishida Shinya , Murata Masayuki 
Published: January 2012

SUMMARYWavelength‐routed Generalized Multi‐Protocol Label Switching (GMPLS) networks use Resource reSerVation Protocol—Traffic Engineering (RSVP‐TE) as signaling protocol to set up and tear down lightpaths. RSVP‐TE uses a soft‐state control mechanism to manage lightpaths. In the soft‐state control mechanism, each node sets a timer for each control state and resets the timer with refresh messages to maintain the state. When the timer expires due to losses of refresh messages, the control state is initialized and a reserved resource managed with the state is released. It has been considered that resource utilization of soft‐state protocols is inferior to that of hard‐state protocols, since soft‐state protocols may reserve resources until control states are deleted due to timeout. Therefore, some extensions to promote the performance of soft‐state protocols, such as message retransmission, have been considered. In this paper, we analyze the behavior of GMPLS RSVP‐TE and its variants with a Markov model and analyze the performance of RSVP‐TE. From the results, we demonstrate that resource utilization of RSVP‐TE can be equivalent to that of a hard‐state protocol when the loss probability of signaling messages is low. We also investigate the effectiveness of message retransmission and show that using message retransmission leads to poor resource utilization in some cases. Copyright © 2012 John Wiley & Sons, Ltd.
From Proteomics Data Representation to Public Data Flow: A Report on the HUPO‐PSI Workshop September 2011, Geneva, Switzerland
Authors: Orchard Sandra , Albar Juan‐Pablo , Deutsch Eric W. , Eisenacher Martin , Binz Pierre‐Alain , Martinez‐Bartolomé Salvador , Vizcaíno Juan Antonio , Hermjakob Henning 
Published: January 2012

AbstractThe plenary session of the Proteomics Standards Initiative (PSI) of the Human Proteome Organization at the Tenth annual HUPO World Congress updated the delegates on the ongoing activities of this group. The Molecular Interactions workgroup described the success of the PSICQUIC web service, which enables users to access multiple interaction resources with a single query. One user instance is the IMEx Consortium, which uses the service to enable users to access a non‐redundant set of protein–protein interaction records. The mass spectrometry data formats, mzML for mass spectrometer output files and mzIdentML for the output of search engines, are now successfully established with increasing numbers of implementations. A format for the output of quantitative proteomics data, mzQuantML, and also TraML, for SRM/MRM transition lists, are both currently nearing completion. The corresponding MIAPE documents are being updated in line with advances in the field, as is the shared controlled vocabulary PSI‐MS. In addition, the mzTab format was introduced, as a simpler way to report MS proteomics and metabolomics results. Finally, the ProteomeXchange Consortium, which will supply a single entry point for the submission of MS proteomics data to multiple data resources including PRIDE and PeptideAtlas, is currently being established.
Boosting the Globalization of Plant Proteomics through INPPO: Current Developments and Future Prospects
Authors: Agrawal Ganesh Kumar , Sarkar Abhijit , Agrawal Raj , Ndimba Bongani Kaiser , Tanou Georgia , Dunn Michael J. , Kieselbach Thomas , Cramer Rainer , Wienkoop Stefanie , Chen Sixue , Rafudeen Mohammed Suhail , Deswal Renu , Barkla Bronwyn J. , Weckwerth Wolfram , Heazlewood Joshua L. , Renaut Jenny , Job Dominique , Chakraborty Niranjan , Rakwal Randeep 
Published: January 2012

AbstractThe International Plant Proteomics Organization (INPPO) is a non‐profit‐organization consisting of people who are involved or interested in plant proteomics. INPPO is constantly growing in volume and activity, which is mostly due to the realization among plant proteomics researchers worldwide for the need of such a global platform. Their active participation resulted in the rapid growth within the first year of INPPO's official launch in 2011 via its website (www.inppo.com) and publication of the ‘Viewpoint paper’ in a special issue of PROTEOMICS (May 2011). Here, we will be highlighting the progress achieved in the year 2011 and the future targets for the year 2012 and onwards. INPPO has achieved a successful administrative structure, the Core Committee (CC; composed of President, Vice‐President, and General Secretaries), Executive Council (EC), and General Body (GB) to achieve INPPO objectives. Various committees and subcommittees are in the process of being functionalized via discussion amongst scientists around the globe. INPPO's primary aim to popularize the plant proteomics research in biological sciences has also been recognized by PROTEOMICS where a section dedicated to plant proteomics has been introduced starting January 2012, following the very first issue of this journal devoted to plant proteomics in May 2011. To disseminate organizational activities to the scientific community, INPPO has launched a biannual (in January and July) newsletter entitled ‘INPPO Express: News & Views’ with the first issue published in January 2012. INPPO is also planning to have several activities in 2012, including programs within the Education Outreach committee in different countries, and the development of research ideas and proposals with priority on crop and horticultural plants, while keeping tight interactions with proteomics programs on model plants such as Arabidopsis thaliana, rice, and Medicago truncatula. Altogether, the INPPO progress and upcoming activities are because of immense support, dedication, and hard work of all members of the INPPO community, and also due to the wide encouragement and support from the communities (scientific and non‐scientific).
Are psychosocial factors associated with quality of life in patients with haematological cancer? A critical review of the literature
Authors: Allart Priscilla , Soubeyran Pierre , Cousson‐Gélie Florence 
Published: January 2012

AbstractBackgroundHaematological cancers differ from other cancers mainly with regard to treatment strategies: surgery is used for diagnostic purposes but rarely for treatment, whereas chemotherapy is of central importance and, in some cases, cures patients. This article reviews studies that examine the relationships between psychosocial factors and quality of life (QoL) in haematological cancer patients.MethodsA review of the literature was conducted from the databases ‘PsycInfo’, ‘Medline’ and ‘Science Direct’ using the keywords ‘lymphoma’, ‘leukaemia’, ‘myeloma’, ‘quality of life’, ‘psychosocial factors’, ‘coping’, ‘social support’, ‘personality’, ‘anxiety,’ ‘depression’, ‘locus of control’ and ‘alexithymia’.ResultsFourteen studies were analysed. One study found positive relationships between sense of coherence and health‐related QoL, whereas another showed a positive link between self‐esteem and QoL. Another study suggested that a high external health locus of control was related to a better QoL. Fighting spirit had a positive impact on QoL for two studies, and helplessness–hopelessness was positively related to emotional distress in one study. Two studies indicated the relationships between emotional distress and QoL. Satisfaction with information about disease determined emotional distress in another study. Social support, general health perceptions, global meaning or spirituality were found to improve QoL in four other reports.ConclusionLiterature about the relationships between psychosocial factors and QoL is lacking. Sense of coherence, self‐esteem and health locus of control, coping strategies, social support, global meaning or emotional distress are associated with QoL. Results concerning coping and social support should be interpreted with caution because of level II evidence in studies. Copyright © 2012 John Wiley & Sons, Ltd.
Effect of closed endotracheal tube suction method, catheter size, and post‐suction recruitment during high‐frequency jet ventilation in an animal model
Authors: Hepponstall J. Michele , Tingay David G. , Bhatia Risha , Loughnan Peter M. , Copnell Beverley 
Published: January 2012

AbstractRationaleHigh‐frequency jet ventilation (HFJV) is often used to treat infants with pathologies associated with gas trapping and abnormal lung mechanics, who are sensitive to the adverse effects of suction.ObjectiveThis study aimed to investigate the effect of closed suction (CS), catheter size, and the use of active post‐suction sighs on tracheal pressure (Ptrach), and global and regional end‐expiratory lung volume (EELV) during HFJV.MethodsSix anaesthetized and muscle‐relaxed adult rabbits were stabilized on HFJV. CS was performed using all permutations of three CS methods (Continual negative pressure, negative pressure applied during Withdrawal, and HFJV in Standby) and 6 French gauge (6FG) and 8 French gauge (8FG) catheter, randomly assigned. The sequence was repeated using post‐suction sighs. Ptrach, absolute (respiratory inductive plethysmography) and regional (electrical impedance tomography; expressed as percentage of vital capacity for the defined region of interest, %ZVCroi) EELV were measured before, during and 60 sec post‐suction.ResultsCS methods exerted no difference on ΔPtrach, ΔEELVRIP, or Δ%ZVCroi. 8FG catheter resulted in a mean (95%CI) 20.0 (17.9,22.2) cm H2O greater loss of Ptrach during suction compared to 6FG (Bonferroni post‐test). Mean (±SD) ΔEELVRIP was −6(±3) and −2(±1) ml/kg with the 8 and 6FG catheters (P < 0.0001; Bonferroni post‐test). ΔEELV was 31.7 (21.1,42.4) %ZVCroi and 24.8 (10.9,38.7) %ZVCroi greater in the ventral and dorsal hemithoraces using the 8FG. Only after 8FG CS was post‐suction recruitment required to restore EELV.ConclusionsIn this animal model receiving HFJV, ΔPtrach, ΔEELV, and need for post‐suction recruitment during CS were most influenced by catheter size. Volume changes within the lung were uniform. Pediatr Pulmonol. 2012. 47:749–756. © 2012 Wiley Periodicals, Inc.
Necrotizing sarcoid granulomatosis of the lung in a 12‐year‐old boy with an atypical clinical course
Authors: Panigada Serena , Ullmann Nicola , Sacco Oliviero , Gambini Claudio , Bush Andrew , Rossi Giovanni A. 
Published: January 2012

AbstractNecrotizing sarcoid granulomatosis (NSG) is a disorder of unknown etiology, rarely described in childhood, belonging to the heterogeneous group of the pulmonary angiitis and granulomatosis. One of the characteristics of NSG is to have typically a benign clinical course with minimal treatment with systemic steroids or even with no therapy at all. Here, we report the case of a boy with a lung consolidation, with morphological and histological features consistent with a diagnosis of NSG. Good clinical and roentgenographic response to high dose prednisone treatment was followed three times by relapses, when steroid treatment was tapered. New lesions were detected in different areas of the lung and not in initially affected area, never previously described in NSG and only rarely in other pulmonary angiitides. Pediatr Pulmonol. 2012. 47:831–835. © 2012 Wiley Periodicals, Inc.
Testosterone promotes DNA damage response under oxidative stress in prostate cancer cell lines
Authors: Ide Hisamitsu , Lu Yan , Yu Jingsong , China Toshiyuki , Kumamoto Tomoka , Koseki Tatsuro , Yamaguchi Raizo , Muto Satoru , Horie Shigeo 
Published: January 2012

AbstractBACKGROUNDSustained chronic inflammation and oxidative stress in the prostate promote prostate carcinogenesis. The process of oncogenic transformation leads to enhanced DNA damage and activates the checkpoint network that functions as an inducible barrier against cancer progression. Here, we analyzed the effects of testosterone on the DNA damage response in prostate cancer cells to assess whether testosterone functions a barrier to cancer progression under the oxidative stress.METHODSWe examined the effects of testosterone on components of the DNA damage response pathway, including ATM (ataxia‐telangiectasia‐mutated kinase), H2AX (histone H2AX variant), and Chk2 (checkpoint kinase2) in prostate cancer cell lines, treated with various concentration of hydrogen peroxide (H2O2). Cellular apoptosis was quantified by poly (ADP‐ribose) polymerase (PARP) cleavage and flow cytometry.RESULTSH2O2 induced apoptosis and phosphorylation of ATM, Chk2, and H2AX in LNCaP cells. An ATM inhibitor, Ku55933, reduced H2O2‐induced apoptosis in LNCaP and 22Rv1 cells. Androgen treatments increased H2O2‐induced activation of the DNA damage response and PARP cleavage, but not when the H2O2‐treated cells were also treated with the anti‐androgen flutamide. The ATM inhibitor Ku55933 inhibited androgen‐induced phosphorylation of ATM and PARP cleavage.CONCLUSIONSDNA damage responses play important roles in the maintenance of the cell homeostasis in response to oxidative stress. Our results indicated that under oxidative stress androgen signaling may induce apoptosis by activating the DNA damage response. Prostate 72:1407–1411, 2012. © 2012 Wiley Periodicals, Inc.
Glycoprotein transmembrane nmb: An androgen‐downregulated gene attenuates cell invasion and tumorigenesis in prostate carcinoma cells
Authors: Tsui Ke‐Hung , Chang Ying‐Ling , Feng Tsui‐Hsia , Chang Phei‐Lang , Juang Horng‐Heng 
Published: January 2012

AbstractBACKGROUNDGlycoprotein transmembrane nmb (GPNMB) gene was originally identified in osteoblasts and belongs to the pmel‐17/nmb family. The function or regulation of GPNMB in the human prostate remains unknown.METHODSThe expression of GPNMB in prostate carcinoma cells were determined by real‐time reverse transcription‐polymerase chain reaction (RT‐qPCR) and immunoblot assays. Effects of ectopic GPNMB overexpression on cell proliferation, invasion, and tumorigenesis were determined by 3H‐thymidine incorporation, matrigel invasion, soft agar cloning assays, and murine xenograft study. Effects of GPNMB, p53, and androgen on target gene were assessed using RT‐PCR, immunoblotting, and transient gene expression assays.RESULTSIn vitro analysis using several prostate cell lines suggested that expression of GPNMB may be relevant to the extent of neoplasia. Ectopic overexpression of GPNMB significantly attenuated cell proliferation and invasion and exerted antitumorigenic activity on PC‐3 cells in vitro and in vivo. GPNMB overexpression induced the gene expressions of N‐myc downstream regulated gene 1 (Ndrg1) and maspin in PC‐3 cells. Doxorubicin treatment or transient overexpression of p53 increased GPNMB expression. Androgen (R1881) treatment has a divergent effect on gene expression of prostate‐specific antigen (PSA) and GPNMB in LNCaP cells. Androgen treatment enhanced cell proliferation but downregulated GPNMB protein expression in stably overexpressed androgen receptor (AR) CA‐HPV‐10 cells.CONCLUSIONSTogether these results suggest that GPNMB gene is a p53‐ and androgen‐dysregulated gene and should be regarded as an anti‐tumor gene for prostate cancer. The enhancement of Ndrg1 and maspin gene expressions may account for the anti‐proliferative and anti‐invasive function of GPNMB in PC‐3 cells. Prostate 72:1431–1442, 2012. © 2012 Wiley Periodicals, Inc.
Variability of retention process of isoxaflutole and its diketonitrile metabolite in soil under conventional and conservation tillage
Authors: Alletto Lionel , Benoit Pierre , Bergheaud Valérie , Coquet Yves 
Published: January 2012

AbstractBACKGROUND: Sorption largely controls pesticide fate in soils because it influences its availability for biodegradation or transport in the soil water. In this study, variability of sorption and desorption of isoxaflutole (IFT) and its active metabolite diketonitrile (DKN) was investigated under conventional and conservation tillage.RESULTS: According to soil samples, IFT KD values ranged from 1.4 to 3.2 L kg−1 and DKN KD values ranged from 0.02 to 0.17 L kg−1. Positive correlations were found between organic carbon content and IFT and DKN sorption. IFT and DKN sorption was higher under conservation than under conventional tillage owing to higher organic carbon content. Under conservation tillage, measurements on maize and oat residues collected from the soil surface showed a greater sorption of IFT on plant residues than on soil samples, with the highest sorbed quantities measured on maize residues (KD ≈ 45 L kg−1). Desorption of IFT was hysteretic, and, after five consecutive desorptions, between 72 and 89% of the sorbed IFT was desorbed from soil samples. For maize residues, desorption was weak (<50% of the sorbed IFT), but, after two complementary desorptions allowing for IFT hydrolysis, DKN was released from maize residues.CONCLUSION: Owing to an increase in organic carbon in topsoil layers, sorption of IFT and DKN was enhanced under conservation tillage. Greater sorption capacities under conservation tillage could help in decreasing DKN leaching to groundwater. Copyright © 2012 Society of Chemical Industry