bluuebunny/crossref_metadata_2025_split huggingface dataset:
Phylogenomic analyses of malaria parasites and evolution of their exported proteins
Authors: Pick Christian , Ebersberger Ingo , Spielmann Tobias , Bruchhaus Iris , Burmester Thorsten 
Published: June 2011

Abstract

Background

Plasmodium falciparum is the most malignant agent of human malaria. It belongs to the taxon Laverania, which includes other ape-infecting Plasmodium species. The origin of the Laverania is still debated. P. falciparum exports pathogenicity-related proteins into the host cell using the Plasmodium export element (PEXEL). Predictions based on the presence of a PEXEL motif suggest that more than 300 proteins are exported by P. falciparum, while there are many fewer exported proteins in non-Laverania.

Results
A whole-genome approach was applied to resolve the phylogeny of eight Plasmodium species and four outgroup taxa. By using 218 orthologous proteins we received unanimous support for a sister group position of Laverania and avian malaria parasites. This observation was corroborated by the analyses of 28 exported proteins with orthologs present in all Plasmodium species. Most interestingly, several deviations from the P. falciparum PEXEL motif were found to be present in the orthologous sequences of non-Laverania.

Conclusion
Our phylogenomic analyses strongly support the hypotheses that the Laverania have been founded by a single Plasmodium species switching from birds to African great apes or vice versa. The deviations from the canonical PEXEL motif in orthologs may explain the comparably low number of exported proteins that have been predicted in non-Laverania.
The influence of both individual and area based socioeconomic status on temporal trends in Caesarean sections in Scotland 1980-2000
Authors: Fairley Lesley , Dundas Ruth , Leyland Alastair H 
Published: June 2011

Abstract

Background
Caesarean section rates have risen over the last 20 years. Elective Caesarean section rates have been shown to be linked to area deprivation in England, women in the most deprived areas were less likely to have an elective section than those in the most affluent areas. We examine whether individual social class, area deprivation or both are related to Caesarean sections in Scotland and investigate changes over time.

Methods
Routine maternity discharge data from live singleton births in Scottish hospitals from three time periods were used; 1980-81 (n = 133,555), 1990-91 (n = 128,933) and 1999-2000 (n = 102,285). Multilevel logistic regression, with 3 levels (births, postcode sector and Health Board) was used to analyse emergency and elective Caesareans separately; analysis was further stratified by previous Caesarean section. The relative index of inequality (RII) was used to assess socioeconomic inequalities.

Results
Between 1980-81 and 1999-2000 the emergency section rate increased from 6.3% to 11.9% and the elective rate from 3.6% to 5.5%. In 1980-81 and 1990-91 emergency Caesareans were more likely among women at the bottom of the social class hierarchy compared to those at the top (RII = 1.14, 95%CI 1.00-1.25 and RII = 1.13, 1.03-1.23 respectively) and also among women in the most deprived areas compared to those in the most affluent (RII = 1.18, 1.05-1.32 and RII = 1.13, 1.02-1.26 respectively). In 1999-2000 the odds of an elective section were lower for women at the bottom of the social class hierarchy than those at the top (RII = 0.87, 0.76-1.00) and also lower in women in the most deprived areas compared to those in the most affluent (RII = 0.85, 0.73-0.99).

Conclusions
Both individual social class and area deprivation are independently associated with Caesarean sections in Scotland. The tendency for disadvantaged women to be more likely to receive emergency sections disappeared at the same time as the likelihood of advantaged groups receiving elective sections increased.
Diarrheal disease risk in rural Bangladesh decreases as tubewell density increases: a zero-inflated and geographically weighted analysis
Authors: Carrel Margaret , Escamilla Veronica , Messina Jane , Giebultowicz Sophia , Winston Jennifer , Yunus Mohammad , Streatfield PKim , Emch Michael 
Published: June 2011

AbstractBackgroundThis study investigates the impact of tubewell user density on cholera and shigellosis events in Matlab, Bangladesh between 2002 and 2004. Household-level demographic, health, and water infrastructure data were incorporated into a local geographic information systems (GIS) database. Geographically-weighted regression (GWR) models were constructed to identify spatial variation of relationships across the study area. Zero-inflated negative binomial regression models were run to simultaneously measure the likelihood of increased magnitude of disease events and the likelihood of zero cholera or shigellosis events. The aim of this study was to examine the effect of tubewell density on both the occurrence of diarrheal disease and the magnitude of diarrheal disease incidence.ResultsIn Matlab, households with greater tubewell density were more likely to report zero cholera or shigellosis events. Results for both cholera and shigellosis GWR models suggest that tubewell density effects are spatially stationary and the use of non-spatial statistical methods is appropriate.ConclusionsIncreasing the amount of drinking water available to households through increased density of tubewells contributed to lower reports of cholera and shigellosis events in rural Bangladesh. Our findings demonstrate the importance of tubewell installation and access to groundwater in reducing diarrheal disease events in the developing world.
CdSe/TiO2 core-shell nanoparticles produced in AOT reverse micelles: applications in pollutant photodegradation using visible light
Authors: Fontes Garcia Arlindo M , Fernandes Marisa SF , Coutinho Paulo JG 
Published: June 2011

Abstract
CdSe quantum dots with a prominent band-edge photoluminescence were obtained by a soft AOT water-in-oil (w/o) microemulsion templating method with an estimated size of 2.7 nm. The CdSe particles were covered with a TiO2 layer using an intermediate SiO2 coupling reagent by a sol-gel process. The resulting CdSe/TiO2 core/shell nanoparticles showed appreciable photocatalytic activity at λ = 405 nm which can only originate because of electron injection from the conduction band of CdSe to that of TiO2.
The role of particle size of particulate nano-zinc oxide wood preservatives on termite mortality and leach resistance
Authors: Clausen Carol A , Kartal S Nami , Arango Rachel A , Green Frederick 
Published: June 2011

AbstractHistorically most residential wood preservatives were aqueous soluble metal formulations, but recently metals ground to submicron size and dispersed in water to give particulate formulations have gained importance. In this study, the specific role nano-zinc oxide (ZnO) particle size and leach resistance plays in termite mortality resulting from exposure to particulate ZnO-treated wood was investigated. Southern yellow pine (SYP) sapwood impregnated with three concentrations of two particle sizes (30 and 70 nm) of ZnO were compared to wood treated with soluble zinc sulphate (ZnSO4) preservative for leach resistance and termite resistance. Less than four percent leached from the particulate nano-ZnO-treated specimens, while 13 to 25% of the zinc sulphate leached from the soluble treated wood. Nano-ZnO was essentially non-leachable from wood treated with 5% formulation for the 30-nm particle size. In a no-choice laboratory test, eastern subterranean termites (Reticulitermes flavipes) consumed less than 10% of the leached nano-ZnO-treated wood with 93 to 100% mortality in all treatment concentrations. In contrast, termites consumed 10 to 12% of the leached ZnSO4-treated wood, but with lower mortality: 29% in the 1% treatment group and less than 10% (5 and 8%, respectively) in the group of wood blocks treated with 2.5 and 5.0% ZnSO4. We conclude that termites were repelled from consuming wood treated with nano-ZnO, but when consumed it was more toxic to eastern subterranean termites than wood treated with the soluble metal oxide formulation. There were no differences in leaching or termite mortality between the two particle sizes of nano-ZnO.
A metabolic link between mitochondrial ATP synthesis and liver glycogen metabolism: NMR study in rats re-fed with butyrate and/or glucose
Authors: Gallis Jean-Louis , Gin Henri , Roumes Hélène , Beauvieux Marie-Christine 
Published: June 2011

Abstract

Background
Butyrate, end-product of intestinal fermentation, is known to impair oxidative phosphorylation in rat liver and could disturb glycogen synthesis depending on the ATP supplied by mitochondrial oxidative phosphorylation and cytosolic glycolysis.

Methods
In 48 hr-fasting rats, hepatic changes of glycogen and total ATP contents and unidirectional flux of mitochondrial ATP synthesis were evaluated by ex vivo
31P NMR immediately after perfusion and isolation of liver, from 0 to 10 hours after force-feeding with (butyrate 1.90 mg + glucose 14.0 mg.g-1 body weight) or isocaloric glucose (18.2 mg.g-1 bw); measurements reflected in vivo situation at each time of liver excision. The contribution of energetic metabolism to glycogen metabolism was estimated.

Results
A net linear flux of glycogen synthesis (~11.10 ± 0.60 μmol glucosyl units.h-1.g-1 liver wet weight) occurred until the 6th hr post-feeding in both groups, whereas butyrate delayed it until the 8th hr. A linear correlation between total ATP and glycogen contents was obtained (r2 = 0.99) only during net glycogen synthesis. Mitochondrial ATP turnover, calculated after specific inhibition of glycolysis, was stable (~0.70 ± 0.25 μmol.min-1.g-1 liver ww) during the first two hr whatever the force-feeding, and increased transiently about two-fold at the 3rd hr in glucose. Butyrate delayed the transient increase (1.80 ± 0.33 μmol.min-1.g-1 liver ww) to the 6th hr post-feeding. Net glycogenolysis always appeared after the 8th hr, whereas flux of mitochondrial ATP synthesis returned to near basal level (0.91 ± 0.19 μmol.min-1.g-1 liver ww).

Conclusion
In liver from 48 hr-starved rats, the energy need for net glycogen synthesis from exogenous glucose corresponds to ~50% of basal mitochondrial ATP turnover. The evidence of a late and transient increase in mitochondrial ATP turnover reflects an energetic need, probably linked to a glycogen cycling. Butyrate, known to reduce oxidative phosphorylation yield and to induce a glucose-sparing effect, delayed the transient increase in mitochondrial ATP turnover and hence energy contribution to glycogen metabolism.
DNA binding activities of the Herves transposase from the mosquito Anopheles gambiae
Authors: Kahlon Amandeep S , Hice Robert H , O'Brochta David A , Atkinson Peter W 
Published: June 2011

Abstract

Background
Determining the mechanisms by which transposable elements move within a genome increases our understanding of how they can shape genome evolution. Class 2 transposable elements transpose via a 'cut-and-paste' mechanism mediated by a transposase that binds to sites at or near the ends of the transposon. Herves is a member of the hAT superfamily of class 2 transposons and was isolated from Anopheles gambiae, a medically important mosquito species that is the major vector of malaria in sub-Saharan Africa. Herves is transpositionally active and intact copies of it are found in field populations of A gambiae. In this study we report the binding activities of the Herves transposase to the sequences at the ends of the Herves transposon and compare these to other sequences recognized by hAT transposases isolated from other organisms.

Results
We identified the specific DNA-binding sites of the Herves transposase. Active Herves transposase was purified using an Escherichia coli expression system and bound in a site-specific manner to the subterminal and terminal sequences of the left and right ends of the element, respectively, and also interacted with the right but not the left terminal inverted repeat. We identified a common subterminal DNA-binding motif (CG/AATTCAT) that is critical and sufficient for Herves transposase binding.

Conclusions
The Herves transposase binds specifically to a short motif located at both ends of the transposon but shows differential binding with respect to the left and right terminal inverted repeats. Despite similarities in the overall structures of hAT transposases, the regions to which they bind in their respective transposons differ in sequence ensuring the specificity of these enzymes to their respective transposon. The asymmetry with which the Herves terminal inverted repeats are bound by the transposase may indicate that these differ in their interactions with the enzyme.
Gender-related outcome difference is related to course of sepsis on mixed ICUs: a prospective, observational clinical study
Authors: Nachtigall Irit , Tafelski Sascha , Rothbart Andreas , Kaufner Lutz , Schmidt Maren , Tamarkin Andrey , Kartachov Maxim , Zebedies Daniela , Trefzer Tanja , Wernecke Klaus-Dieter , Spies Claudia 
Published: June 2011

Introduction
Introduction
Impact of gender on severe infections is in highly controversial discussion with natural survival advantage of females described in animal studies but contradictory to those described human data. This study aims to describe the impact of gender on outcome in mixed intensive care units (ICUs) with a special focus on sepsis.

Methods
We performed a prospective, observational, clinical trial at Charité University Hospital in Berlin, Germany. Over a period of 180 days, patients were screened, undergoing care in three mainly surgical ICUs. In total, 709 adults were included in the analysis, comprising the main population ([female] n = 309, [male] n = 400) including 327 as the sepsis subgroup ([female] n = 130, [male] n = 197).

Results
Basic characteristics differed between genders in terms of age, lifestyle factors, comorbidities, and SOFA-score (Sequential Organ Failure Assessment). Quality and quantity of antibiotic therapy in means of antibiotic-free days, daily antibiotic use, daily costs of antibiotics, time to antibiotics, and guideline adherence did not differ between genders. ICU mortality was comparable in the main population ([female] 10.7% versus [male] 9.0%; P = 0.523), but differed significantly in sepsis patients with [female] 23.1% versus [male] 13.7% (P = 0.037). This was confirmed in multivariate regression analysis with OR = 1.966 (95% CI, 1.045 to 3.701; P = 0.036) for females compared with males.

Conclusions
No differences in patients' outcome were noted related to gender aspects in mainly surgical ICUs. However, for patients with sepsis, an increase of mortality is related to the female sex.
Impact of Patient-Reported Outcomes in Oncology: A Longitudinal Analysis of Patient-Physician Communication
Authors: Takeuchi Elena E. , Keding Ada , Awad Noha , Hofmann Ursula , Campbell Lyndsay J. , Selby Peter J. , Brown Julia M. , Velikova Galina 
Published: June 2011

Purpose Regularly collecting patient-reported outcomes (PROs) of health-related quality of life with feedback to oncologists may assist in eliciting and monitoring patients' problems during cancer treatment. This study examined how PRO feedback had an impact on patient-physician communication over time to gain a better understanding of how it may influence patient care. Patients and Methods Exploratory analyses were performed on a data set from a previous study. Patients were randomly assigned to intervention (regular completion of European Organisation for Research and Treatment of Cancer Quality of Life Questionnaire-Core 30 and Hospital Anxiety and Depression Scale with feedback to oncologists), attention-control (completion of same questionnaires without feedback), and control (standard care) arms. The content of consultation audio recordings between 28 oncologists and 198 patients over four consecutive visits (792 consultations) was analyzed. Mixed-effects models and multivariate regressions were used to examine the longitudinal impact of the intervention on patient-physician communication, dynamics of patient-physician interaction, and the association between PROs and the content of clinic discussion. Results Patients in the intervention arm discussed more symptoms over time compared with patients in the attention-control (P = .008) and control (P = .04) arms. No study arm effect was observed for function discussions. Discussion topics were predominantly raised by patients/relatives, regardless of arm allocation. Clinic discussions were associated with severity of patient-reported symptoms but not with patient-reported functional concerns. Conclusion A positive longitudinal impact of the intervention on symptom discussion was observed, but not for function discussion, suggesting that potentially serious problems may remain unaddressed. Training oncologists in responding to patient-reported functional concerns may increase the impact of this intervention.
Phase II, Open-Label, Single-Arm Trial of Imatinib Mesylate in Patients With Metastatic Melanoma Harboring c-Kit Mutation or Amplification
Authors: Guo Jun , Si Lu , Kong Yan , Flaherty Keith T. , Xu Xiaowei , Zhu Yanyan , Corless Christopher L. , Li Li , Li Haifu , Sheng Xinan , Cui Chuanliang , Chi Zhihong , Li Siming , Han Mei , Mao Lili , Lin Xuede , Du Nan , Zhang Xiaoshi , Li Junling , Wang Baocheng , Qin Shukui 
Published: June 2011

Purpose Melanomas harbor aberrations in the c-Kit gene. We tested the efficiency of the tyrosine kinase inhibitor imatinib in selected patients with metastatic melanoma harboring c-Kit mutations or amplifications. Patients and Methods Forty-three patients with metastatic melanoma harboring c-Kit aberrations were enrolled on this phase II trial. Each patient received a continuous dose of imatinib 400 mg/d unless intolerable toxicities or disease progression occurred. Fifteen patients who experienced progression of disease were allowed to escalate the dose to 800 mg/d. Results Forty-three patients were eligible for evaluation, and the median follow-up time was 12.0 months. The median progression-free survival (PFS) was 3.5 months, and the 6-month PFS rate was 36.6%. Rate of total disease control was 53.5%: 10 patients (23.3%; 95% CI, 10.2% to 36.4%) and 13 patients (30.2%; 95% CI, 16.0% to 44.4%) achieved partial response (PR) and stable disease (SD), respectively. Eighteen patients (41.9%) demonstrated regression of tumor mass. Notably, nine of the 10 PRs were observed in patients with mutations in exons 11 or 13. The 1-year overall survival (OS) rate was 51.0%. The median PFS and OS times for patients who had PR or SD versus disease progression were 9.0 months versus 1.5 months (P < .001) and 15.0 months versus 9.0 months (P = .036), respectively. Imatinib 400 mg/d was well tolerated, and only one of the 15 patients who received dose escalation to 800 mg/d achieved SD. Conclusion Imatinib demonstrated significant activity in patients with metastatic melanoma harboring genetic c-Kit aberrations, with an overall response rate of 23.3%. Escalation to 800 mg/d could not restore disease control.
Exercise Behavior, Functional Capacity, and Survival in Adults With Malignant Recurrent Glioma
Authors: Ruden Emily , Reardon David A. , Coan April D. , Herndon James E. , Hornsby Whitney E. , West Miranda , Fels Diane R. , Desjardins Annick , Vredenburgh James J. , Waner Emily , Friedman Allan H. , Friedman Henry S. , Peters Katherine B. , Jones Lee W. 
Published: June 2011

Purpose Identifying strong markers of prognosis are critical to optimize treatment and survival outcomes in patients with malignant recurrent glioma. We investigated the prognostic significance of exercise behavior and functional capacity in this population. Patients and Methods Using a prospective design, 243 patients with WHO grades 3 to 4 recurrent malignant glioma and Karnofsky performance status (KPS) ≥ 70 completed a self-administered questionnaire that assessed exercise behavior and performed a 6-minute walk test (6MWT) to assess functional capacity. Cox proportional models were used to estimate the risk of all-cause mortality according to 6MWT distance (6MWD; < 390 meters, 390-489 meters, > 489 meters) and exercise behavior (metabolic equivalent [MET] –h/wk) adjusted for KPS and other important clinical factors. Results Median follow-up was 27.43 months. During this period, 149 deaths were recorded (61% of the total sample). Exercise behavior was an independent predictor of survival (P = .0081). Median survival was 13.03 months for patients reporting < 9 MET-h/wk relative to 21.84 months for those reporting ≥ 9 MET-h/wk. Exercise behavior added incremental prognostic value beyond that provided by KPS, age, sex, grade, and number of prior progressions (P < .001). Compared with patients reporting < 9 MET-h/wk, the adjusted hazard ratio for mortality was 0.64 (95% CI, 0.46 to 0.91) for patients reporting ≥ 9 MET-h/wk. Functional capacity was not an independent predictor of prognosis. Conclusion Exercise behavior is a strong independent predictor of survival that provides incremental prognostic value to KPS as well as traditional markers of prognosis in malignant recurrent glioma.
An Experimental Investigation of a Large, Vertical-Axis Wind Turbine: Effects of Venting and Capping
Authors: Plourde B. D. , Abraham J. P. , Mowry G. S. , Minkowycz W.J. 
Published: June 2011

A novel, vertical-axis wind turbine has been developed for the use in powering cellular communication towers. The new turbine takes advantage of the elevated heights of existing towers to access relatively high-speed winds. The new turbine is designed to provide local power to the cellular communication system, the electronics are designed so that the turbine stores power in a battery system when excess power is generated. This stored power is utilized at times when the wind-speed is insufficient to power the communication system. A major opportunity exists for communication towers which are located away from grid-connected electricity. In these cases, the towers are often powered by diesel generators. Through the use of turbines, it is possible to greatly reduce the diesel-fuel consumption. Wind-tunnel tests have evaluated the potential for vented blades and blade capping for the improvement of power generation. It was found that while venting provides only marginal improvement, capping greatly increases power generation. The results also show that further improvements can be obtained through modification of the electrical system.
Helicopter Rotor Noise in the Merged Tip-Vortex and Blade Interaction Condition
Authors: Wie S. Y. , Im D. K. , Kwon J. H. , Lee D. J. , Chung K. H. , Kim S. B. 
Published: June 2011

BVI (blade vortex interaction) phenomena cause unsteady loading and noise in the helicopter rotor. It is difficult to accurately predict BVI aerodynamics and noise, because rotor wake and tip-vortices are numerically dissipated. In the present study, CFD and a time-marching free-wake coupling analysis were used to describe inflow and outflow without wake dissipation. Rotor noise was also computed using the Farassat formula, derived from the Ffowcs-Williams Hawkings equation. To validate aerodynamics and the noise prediction solver, a two-blade rotor was simulated and the results were compared with experimental data. To investigate the vortex interaction of a multi-blade rotor, a four-blade rotor was also simulated. In a slow descent flight condition, tip vortices are merged at the advancing side. The merged tip-vortex and blade interaction were simulated and are discussed in this paper.