bluuebunny/crossref_metadata_2025_split huggingface dataset:
The conclusions of risk analysis as a basis for deciding between variants through the example of Contract H8 / Ein Fazit zur Risikoanalyse als Grundlage für eine Variantenentscheidung am Beispiel Los H8
Authors: Spiegl Markus , Sander Philip , Pellar Alfred , Maidl Ulrich , Herdina Johann , Feistmantl Klaus 
Published: August 2011

AbstractThe Brenner Eisenbahn GmbH (BEG) was appointed in 1995 to design and construct the new railway line in the Lower Inn Valley between the national border near Kufstein and Innsbruck. In the densely populated Inn Valley, large parts of the line with a planned speed of 250 km/h had to be run through tunnels. Near Jenbach, a semi‐quantitative process was used as part of a risk analysis for the decision between the variants of a shallow, mined special construction and a deep mechanically driven tunnel.Die Brenner Eisenbahn GmbH (BEG) wurde im Jahr 1995 mit der Planung und Errichtung der Neubaustrecke im Unterinntal zwischen der Staatsgrenze bei Kufstein und Innsbruck beauftragt. Im dicht bebauten Inntal mussten große Teile der mit einer Spitzengeschwindigkeit von 250 km/h geplanten Strecke in Tunnellage errichtet werden. Im Bereich Jenbach wurde ein semi‐quantitatives Verfahren im Rahmen der Risikoanalyse zur Variantenentscheidung zwischen einer seicht liegenden, bergmännischen Sonderbauweise und einem tiefliegende Maschinenvortrieb angewandt.
Criteria for the selection of tunnelling method through the example of the Koralm Tunnel / Kriterien zur Wahl der Vortriebsarten am Beispiel des Koralmtunnels
Authors: Moritz Bernd , Wagner Hanns , Mussger Klaus , Handke Dieter , Harer Gerhard 
Published: August 2011

AbstractWith a length of 32.9 km, the Koralm tunnel is the key structure on the route of the new 130 km Koralm railway between Graz and Klagenfurt (Austria). This paper defines the most important boundary conditions for dividing the sections and for selecting the methods of tunnelling (sequential or continuous). The focus of the article is mainly on section KAT 3, in which both tertiary sediments and crystalline rock sequences, including the Lavanttal fault zone, are excavated by sequential and continuous tunnelling. The considerations for this section also include the decision to excavate the sediments and the Lavanttal fault zone using a shield machine with earth pressure components. The criteria for defining the different operating modes are described.Der Koralmtunnel mit einer Länge von 32,9 km stellt das Schlüsselbauwerk entlang der 130 km langen neuen Koralmbahn zwischen Graz und Klagenfurt dar. Der vorliegende Beitrag legt die wesentlichen Randbedingungen zur Einteilung der Baulose und zur Wahl der jeweiligen Vortriebsmethode (zyklisch oder kontinuierlich) dar. Der Fokus ist dabei hauptsächlich auf das Baulos KAT 3 gerichtet, in dem sowohl tertiäre Sedimente als auch kristalline Gesteinsabfolgen mitsamt der Lavanttaler Störungszone im zyklischen und im kontinuierlichen Vortrieb durchörtert werden. Die Betrachtungen für dieses Baulos umfassen auch die Entscheidungsfindung, die Sedimente und die Lavanttaler Störungszone mittels Schildmaschine mit Erddruckkomponenten aufzufahren. Die Kriterien zur Abgrenzung der unterschiedlichen Betriebsmodi werden dargestellt.
TBM versus NATM from the contractor's point of view / TBM versus NÖT aus Sicht des Unternehmers
Authors: Gütter Wolfgang , Jäger Manfred , Rudigier Günther , Weber Wolfgang 
Published: August 2011

AbstractThe selection of the correct method of tunnelling from the contractor's point of view mostly depends on its chance to survive the competition of the marketplace, in addition to a host of technical constraints. The tendering and award conditions also decisively influence whether the technically and economically optimal solution is chosen.There are also other factors like the market situation, availability of suitable tunnelling machinery and personnel resources, which can have a significant effect on the decision process.Die Wahl der aus Sicht des Unternehmers richtigen Vortriebsmethode hängt neben einer Vielzahl technischer Randbedingungen vor allem davon ab, wie dieser damit im Wettbewerb bestehen kann. Dabei beeinflussen die Ausschreibungs‐ und Vergabebedingungen ganz wesentlich, ob letztlich auch die technisch und wirtschaftlich optimale Lösung zur Ausführung gelangt. Daneben sind es aber auch Faktoren wie Marktsituation, Verfügbarkeit von geeigneten Vortriebsanlagen, Personalressourcen, die im Entscheidungsprozess der Vortriebsmethode eine gravierende Rolle spielen können.
Multipotent stem/progenitor cells in human biliary tree give rise to hepatocytes, cholangiocytes, and pancreatic islets
Authors: Cardinale Vincenzo , Wang Yunfang , Carpino Guido , Cui Cai-Bin , Gatto Manuela , Rossi Massimo , Berloco Pasquale Bartolomeo , Cantafora Alfredo , Wauthier Eliane , Furth Mark E. , Inverardi Luca , Dominguez-Bendala Juan , Ricordi Camillo , Gerber David , Gaudio Eugenio , Alvaro Domenico , Reid Lola 
Published: August 2011

Abstract

Multipotent stem/progenitors are present in peribiliary glands of extrahepatic biliary trees from humans of all ages and in high numbers in hepato-pancreatic common duct, cystic duct, and hilum. They express endodermal transcription factors (e.g., Sox9, SOX17, FOXA2, PDX1, HES1, NGN3, PROX1) intranuclearly, stem/progenitor surface markers (EpCAM, NCAM, CD133, CXCR4), and sometimes weakly adult liver, bile duct, and pancreatic genes (albumin, cystic fibrosis transmembrane conductance regulator [CFTR], and insulin). They clonogenically expand on plastic and in serum-free medium, tailored for endodermal progenitors, remaining phenotypically stable as undifferentiated cells for months with a cell division initially every ≈36 hours and slowing to one every 2-3 days. Transfer into distinct culture conditions, each comprised of a specific mix of hormones and matrix components, yields either cords of hepatocytes (express albumin, CYP3A4, and transferrin), branching ducts of cholangiocytes (expressing anion exchanger-2-AE2 and CFTR), or regulatable C-peptide secreting neoislet-like clusters (expressing glucagon, insulin) and accompanied by changes in gene expression correlating with the adult fate. Transplantation into quiescent livers of immunocompromised mice results in functional human hepatocytes and cholangiocytes, whereas if into fat pads of streptozocin-induced diabetic mice, results in functional islets secreting glucose-regulatable human C-peptide.

Conclusion:

The phenotypes and availability from all age donors suggest that these stem/progenitors have considerable potential for regenerative therapies of liver, bile duct, and pancreatic diseases including diabetes. (Hepatology2011;)
HAb18G/CD147 promotes cell motility by regulating annexin II-activated RhoA and Rac1 signaling pathways in hepatocellular carcinoma cells
Authors: Zhao Pu , Zhang Wei , Wang Shi-Jie , Yu Xiao-Ling , Tang Juan , Huang Wan , Li Yong , Cui Hong-Yong , Guo Yun-Shan , Tavernier Jan , Zhang Si-He , Jiang Jian-Li , Chen Zhi-Nan 
Published: August 2011

Abstract

Tumor cells can move as individual cells in two interconvertible modes: mesenchymal mode and amoeboid mode. Cytoskeleton rearrangement plays an important role in the interconversion. Previously, we reported that HAb18G/CD147 and annexin II are interacting proteins involved in cytoskeleton rearrangement, yet the role of their interaction is unclear. In this study we found that the depletion of HAb18G/CD147 produced a rounded morphology, which is associated with amoeboid movement, whereas the depletion of annexin II resulted in an elongated morphology, which is associated with mesenchymal movement. The extracellular portion of HAb18G/CD147 can interact with a phosphorylation-inactive mutant of annexin II and inhibit its phosphorylation. HAb18G/CD147 inhibits Rho signaling pathways and amoeboid movement by inhibiting annexin II phosphorylation, promotes membrane localization of WAVE2 and Rac1 activation by way of the integrin-FAK-PI3K/PIP3 signaling pathway, and promotes the formation of lamellipodia and mesenchymal movement.

Conclusion:

These results suggest that the interaction of HAb18G/CD147 with annexin II is involved in the interconversion between mesenchymal and amoeboid movement of hepatocellular carcinoma cells. (Hepatology 2011)
Differential contribution of SLC26A9 to Cl− conductance in polarized and non‐polarized epithelial cells
Authors: Ousingsawat Jiraporn , Schreiber Rainer , Kunzelmann Karl 
Published: August 2011

AbstractSLC26 proteins function as anion exchangers and Cl− channels. SLC26A9 has been proposed to be a constitutively active and CFTR‐regulated anion conductance in human bronchial epithelia. This positive interaction between two Cl− channels has been questioned by others and evidence has been provided that CFTR rather inhibits the transport activity of SLC26A9. We therefore examined the functional interaction between CFTR and SLC26A9 in polarized airway epithelial cells and in non‐polarized HEK293 cells expressing CFTR and SLC26A9. We found that SLC26A9 provides a constitutively active basal Cl− conductance in polarized grown CFTR‐expressing CFBE airway epithelial cells, but not in cells expressing F508del‐CFTR. In polarized CFTR‐expressing cells, SLC26A9 also contributes to both Ca2+‐ and CFTR‐activated Cl− secretion. In contrast in non‐polarized HEK293 cells co‐expressing CFTR/SLC26A9, the baseline Cl− conductance provided by SLC26A9 was inhibited during activation of CFTR. SLC26A9 and CFTR behave differentially in polarized and non‐polarized cells, which may explain earlier conflicting data. J. Cell. Physiol. 227: 2323–2329, 2012. © 2011 Wiley Periodicals, Inc.
Eph‐2B, acting as an extracellular ligand, induces differentiation markers in epidermal keratinocytes
Authors: Walsh Rebecca , Blumenberg Miroslav 
Published: August 2011

AbstractIn the bi‐directional signaling system comprising ephrins (EFNs) and ephrin receptors (Ephs), both EFNs and Ephs simultaneously function both as ligands and as receptors. Importantly, the EFN/Eph system is deregulated in human cancers and has been implicated in the metastatic processes because of its effects on the adhesion and migration of epithelial cells. The idiosyncratic function of Ephs, membrane‐bound receptor kinases, as extracellular signaling ligands, has not been extensively studied. This prompted us to explore the transcriptional targets regulated by Ephs acting solely as ligands. To define the ligand function of EphB2 in human epidermal keratinocytes, we treated these cells with EphB2 as Fc‐conjugate dimmers, which thus act exclusively as extracellular ligands. We compared the EphB2 and EFNA4 effects during a 48 h time course, using transcriptional profiling. We found that EphB2, acting as a ligand, promotes epidermal differentiation. For example, EphB2 induces expression of markers of epidermal differentiation, including keratins KRT1 and KRT10, SPRRs, desmosomal proteins and cell cycle inhibitors, while suppressing basal layer markers, integrins and cell cycle proteins. The effects of EphB2 are delayed relative to those of EFNA4. Unlike EFNA4, EphB2 did not induce lipid metabolism proteins, this particular aspect of epidermal differentiation seems not to be regulated by EphB2. Our results define the transcriptional targets of the reverse signaling by EphB2 acting exclusively as a ligand and begin to characterize this intriguing function of Ephs. J. Cell. Physiol. 227: 2330–2340, 2012. © 2011 Wiley Periodicals, Inc.
Nearest neighbor analysis of dopamine D1 receptors and Na+‐K+‐ATPases in dendritic spines dissected by STED microscopy
Authors: Blom Hans , RöNnlund Daniel , Scott Lena , Spicarova Zuzana , Rantanen Ville , Widengren Jerker , Aperia Anita , Brismar Hjalmar 
Published: August 2011

AbstractProtein localization in dendritic spines is the focus of intense investigations within neuroscience. Applications of super‐resolution microscopy to dissect nanoscale protein distributions, as shown in this work with dual‐color STED, generate spatial correlation coefficients having quite small values. This means that colocalization analysis to some extent looses part of its correlative impact. In this study we thus introduced nearest neighbor analysis to quantify the spatial relations between two important proteins in neurons, the dopamine D1 receptor and Na+,K+‐ATPase. The analysis gave new information on how dense the D1 receptor and Na+,K+‐ATPase constituting nanoclusters are located both with respect to the homogenous (self to same) and the heterogeneous (same to other) topology. The STED dissected nanoscale topologies provide evidence for both a joint as well as a separated confinement of the D1 receptor and the Na+,K+‐ATPase in the postsynaptic areas of dendritic spines. This confined topology may have implications for generation of local sodium gradients and for structural and functional interactions modulating slow synaptic transmission processes. Microsc. Res. Tech., 2011. © 2011 Wiley Periodicals, Inc
Cytotoxic effects of permethrin on mouse liver and spleen cells
Authors: Roma Gislaine Cristina , Oliveira Patrícia Rosa De , Bechara Gervásio Henrique , Mathias Maria Izabel Camargo 
Published: August 2011

AbstractThis study analyzed the histopathological and histochemical effects of different dosages of permethrin on liver and spleen cells of mice, in order to evaluate the toxic potential of this substance and the possible impairments that this chemical causes in different tissues of nontarget organisms (laboratorial conditions). The results showed that permethrin caused severe alterations in the liver cells, reducing the size of the nuclei and causing hydropic degeneration of the hepatocytes, in addition to stimulating the proliferation of Kupffer cells, altered the amount of proteins, polysaccharides, lipids, and vacuoles in the cytoplasm of the hepatocytes and congested the hepatic capillaries. As for the spleen of the treated mice, no alterations were observed in the morphology in relation to the control group, what would suggest that the spleen would continue performing its functions, without suffering morphological alterations even in the presence of the toxic agent. © Microsc. Res. Tech., 2011. © 2011 Wiley Periodicals, Inc.
Fluorescence recognition of proteinaceous binders in works of art by a novel integrated system of investigation
Authors: Sandu Irina Crina Anca , Roque Ana Cecilia A. , Matteini Paolo , Schäfer Stephan , Agati Giovanni , Correia Catarina Ribeiro , Viana Joana Fortio Fernandes Pacheco 
Published: August 2011

AbstractFluorescence microscopy and microspectrofluorometry are important tools in the characterization and identification of proteins, offering a great range of applications in conservation science. Because of their high selectivity and sensitivity, the combination of these techniques can be exploited for improved recognition and quantification of proteinaceous binders in paintings and polychromed works of art. The present article explores an analytical protocol integrating fluorescence microscopy and fluorometry for both identification and mapping of proteinaceous binders (in particular egg and glues) in paint samples. The study has been carried out on historically accurate reconstructions simulating the structure and composition of tempera and oil paints containing these binders. To assess the spatial distribution of specific proteins within the paint layers, cross‐sections from the reconstructions were analyzed by fluorescence imaging after staining with an exogenous fluorophore. Reference fluorescence spectra for each layer were acquired by a multichannel spectral analyzer and compared after Gaussian deconvolution. The results obtained demonstrated the effectiveness of the integrated protocol, highlighting the potential for the use of fluorescent staining coupled with microspectrofluorometry as a routine diagnostic tool in conservation science. The current work creates a set of fully characterized reference samples for further comparison with those from actual works of art. Microsc. Res. Tech., 2011. © 2011 Wiley Periodicals, Inc.
Running in cold weather: morphology, thermal biology, and performance in the southernmost lizard clade in the world (Liolaemus lineomaculatussection: Liolaemini: Iguania)
Authors: Bonino Marcelo Fabián , Azócar Débora Lina Moreno , Tulli María José , Abdala Cristian Simón , Perotti María Gabriela , Cruz Félix Benjamín 
Published: August 2011

AbstractThe integration or coadaptation of morphological, physiological, and behavioral traits is represented by whole‐organism performance traits such as locomotion or bite force. Additionally, maximum sprint speed is a good indicator of whole‐organism performance capacity as variation in sprinting ability can affect survival. We studied thermal biology, morphology, and locomotor performance in a clade ofLiolaemuslizards that occurs in the Patagonian steppe and plateaus, a type of habitat characterized by its harsh cold climate.Liolaemusof thelineomaculatussection display a complex mixture of conservative and flexible traits. The phylogenetically informed analyses of these tenLiolaemusspecies show little coevolution of their thermal traits (only preferred and optimum temperatures were correlated). With regard to performance, maximum speed was positively correlated with optimum temperature. Body size and morphology influenced locomotor performance. Hindlimbs are key for maximal speed, but forelimb length was a better predictor for sustained speed (i.e. average speed over a total distance of 1.2 m). Finally, sustained speed differed among species with different diets, with herbivores running on average faster over a long distance than omnivores.J. Exp. Zool. 315:495–503, 2011. © 2011 Wiley‐Liss, Inc.
Evaluation of the DEMETER performance for seasonal hindcasts of the Indian summer monsoon rainfall
Authors: Ma Shujie , Rodó Xavier , Doblas‐Reyes Francisco J. 
Published: August 2011

AbstractOn the basis of the observed all‐India rainfall (AIR), Climate Prediction Center (CPC) merged analysis of precipitation (CMAP), and the multimodel rainfall simulated in the Development of a European multimodel ensemble system for seasonal to interannual prediction (DEMETER) project, the model performance in terms of forecast quality of the seasonal mean Indian summer monsoon rainfall (ISMR) was evaluated. The approach used included the individual model (single‐model ensemble) comparison as well as its comparison with the multimodel ensemble (MME). The results obtained reveal that systematic biases are the main source of the low skill in predicting the ISMR, while improved reproduction of interannual variability may increase the overall forecast quality for the ISMR. The MME shows superior performance in reproducing AIR features than the single‐model ensembles. This improved forecast quality achieved by the MME with respect to the ISMR variations primarily depends on model performance and secondly on ensemble size. The MME has improved capability of simulating the ISMR variations during 1972–1990 decades, whereas it exhibits a lower performance in the 1990s. The reason of this decadal varying forecast quality clearly deserves more investigation. In summary, these results suggest that the MME's increase in skill absolutely depends on enhancing the individual model quality. Copyright © 2011 Royal Meteorological Society
Lower limb kinematic alterations during drop vertical jumps in female athletes who have undergone anterior cruciate ligament reconstruction
Authors: Delahunt Eamonn , Sweeney Lauren , Chawke Mark , Kelleher Judy , Murphy Katie , Patterson Matt , Prendiville Anna 
Published: August 2011

AbstractThe aim of this study was to determine if anterior cruciate ligament reconstructed (ACL‐R) female athletes exhibit altered lower limb kinematic profiles during jump landing when compared to a non‐injured age, sex, and activity matched control group. Fourteen ACL‐R and 14 non‐injured control subjects performed 3 vertical drop jump (DVJ) trials. Lower limb kinematics were recorded at 200 Hz. Peak and time‐averaged angular displacements were quantified and utilized for between‐group analysis. The ACL‐R group displayed altered hip joint frontal and transverse plane kinematic alterations, and knee joint frontal and sagittal plane kinematic alterations. Specifically the ACL‐R group displayed an increased adducted (p < 0.05) and internally rotated (p < 0.05) hip joint position, both peak and time‐averaged, following landing. The ACL‐R group also displayed a decreased adducted (p < 0.05) and flexed (p < 0.05) position of the knee joint following landing. The observed aberrant lower limb kinematics could pre‐dispose ACL‐R athletes to potential future knee joint injuries. Further studies are required to determine in a prospective manner whether such deficits increase the incidence of recurrent ACL injury, and whether specific sensorimotor protocols following ACL reconstruction can minimize these kinematic deficits. © 2011 Orthopaedic Research Society Published by Wiley Periodicals, Inc. J Orthop Res 30:72–78, 2012
Serological response against myxoma virus and rabbit hemorrhagic disease virus in European wild rabbits using commercial vaccines
Authors: Arenas Antonio J. , Napp Sebastian , Arenas‐Montes Antonio , Borge Carmen , Carbonero Alfonso , Perea Anselmo , Cadenas Rafael , García‐Bocanegra Ignacio 
Published: August 2011

AbstractWe carried out an experimental study to determine the serological response against myxoma virus (MV) and rabbit hemorrhagic disease virus (RHDV) in wild rabbits using commercial vaccines. Seroconversion against MV ranged between 72.7% and 97.2% in animals vaccinated by subcutaneous and intradermal route, respectively, whereas between 75.0% and 77.8% of the animals presented antibodies against RHDV after inoculation with subcutaneous and intradermal vaccines, respectively. Regardless of the inoculation route, vaccination against MV resulted in a significant increase of seropositivity 5 days post‐vaccination (dpv), which did not occur in animals vaccinated against RHDV. Furthermore, seroconversion against MV was significantly higher and faster in intradermally vaccinated rabbits as compared to those inoculated subcutaneously due to either the route of application and/or the type of vaccine used. The results indicated that vaccination significantly increased the prevalence of antibodies against MV and RHDV and suggested that the vaccines currently available induce a safe and effective immune response against both diseases in wild rabbits. Vaccination may be a useful management tool to control both viral diseases in field conditions, particularly in wild rabbits captured for translocations and restocking purposes in which a large number of animals are handled. © 2011 The Wildlife Society.