bluuebunny/crossref_metadata_2025_split huggingface dataset:
Septo‐optic dysplasia and associations with amyoplasia and gastroschisis
Authors: Kamien Benjamin , Zankl Andreas , Gabbett Michael 
Published: March 2010

AbstractBACKGROUND:The causes of septo‐optic dysplasia, amyoplasia, and gastroschisis are mostly unknown.CASES:We present a patient with septo‐optic dysplasia and amyoplasia. We also present a patient with features of septo‐optic dysplasia, gray matter heterotopias, and gastroschisis.CONCLUSIONS:These previously unreported combinations provide further support for vascular disruption in embryonic or fetal life as an etiological factor in these conditions. Birth Defects Research (Part A) 2010. © 2010 Wiley‐Liss, Inc.
Real‐time PCR‐based determination of gene copy numbers in Pichia pastoris
Authors: Abad Sandra , Kitz Kerstin , Hörmann Astrid , Schreiner Ulrike , Hartner Franz S. , Glieder Anton 
Published: March 2010

AbstractPichia pastoris is a preferred host for heterologous protein production. Expression cassettes are usually integrated into the genome of this methylotrophic yeast. This manuscript describes a method for fast and reliable gene copy number determinations for P. pastoris expression strains. We believe that gene copy number determinations are important for all researchers working with P. pastoris and also many other research groups using similar gene integration techniques for the transformation of other yeasts. The described method uses real‐time PCR to quantify the integrated expression cassettes. Similar methods were employed previously for other host systems such as animal and plant cells but no such method comparing different detection methods and describing details for yeast analysis by quantitative PCR is known to us, especially for methylotrophic yeasts such as P. pastoris. Neglecting gene copy numbers can easily lead to false interpretations of experimental results from codon optimization or promoter studies and co‐expression of helper proteins as demonstrated in an application example, which is also described here.
Immobilization conditions of ketoreductase on enantioselective reduction in a gas‐solid bioreactor
Authors: Nagayama Kazuhito , Spiess Antje C. , Büchs Jochen 
Published: March 2010

AbstractThe immobilization conditions of commercial ketoreductase for continuous enantioselective reduction in the gas‐phase reaction were investigated with respect to the immobilization efficiency (residual activity and protein loading) and the gas‐phase reaction efficiency (initial reaction rate, half‐life, and enantioselectivity). For the analyses, ketoreductase was first immobilized by physical deposition on glass supports and the reduction of 2‐butanone to (S)‐2‐butanol with the concomitant regeneration of NADH by 2‐propanol was used as a model reaction. The optimal conditions of enzyme immobilization were obtained using an absolute pressure of 100 hPa for drying, a pH between 6.5 and 7.0, and a buffer concentration of 50 mM. The buffer concentration in particular had a strong effect on both the enzyme activity and enantioselectivity. Under optimal immobilization conditions, the thermostability of ketoreductase in the gas‐phase system was enhanced compared to the aqueous‐phase system, while the enantioselectivity was successfully maintained at a level identical to that of the native enzyme. These results indicate that the gas‐phase reaction has a great potential for industrial production of chiral compounds, but requires careful optimization of immobilization conditions for the reaction to progress effectively.
Virus susceptibility of Chinese hamster ovary (CHO) cells and detection of viral contaminations by adventitious agent testing
Authors: Berting Andreas , Farcet Maria R. , Kreil Thomas R. 
Published: March 2010

AbstractBiopharmaceuticals are of increasing importance in the treatment of a variety of diseases. A remaining concern associated with their production is the potential introduction of adventitious agents into their manufacturing process, which may compromise the pathogen safety of a product and potentially cause stock‐out situations for important medical supplies. To ensure the safety of biological therapeutics, regulatory guidance requires adventitious agent testing (AAT) of the bulk harvest. AAT is a deliberately promiscuous assay procedure which has been developed to indicate, ideally, the presence of any viral contaminant. One of the most important cell lines used in the production of biopharmaceuticals is Chinese hamster ovary (CHO) cells and while viral infections of CHO cells have occurred, a systematic screen of their virus susceptibility has never been published. We investigated the susceptibility of CHO cells to infection by 14 different viruses, including members of 12 families and representatives or the very species that were implicated in previously reported production cell infections. Based on our results, four different infection outcomes were distinguished, based on the possible combinations of the two factors (i) the induction, or not, of a cytopathic effect and (ii) the ability, or not, to replicate in CHO cells. Our results demonstrate that the current AAT is effective for the detection of viruses which are able to replicate in CHO cells. Due to the restricted virus susceptibility of CHO cells and the routine AAT of bulk harvests, our results provide re‐assurance for the very high safety margins of CHO cell‐derived biopharmaceuticals. Biotechnol. Bioeng. 2010;106: 598–607. © 2010 Wiley Periodicals, Inc.
Implementation of communication‐mediating domains for non‐ribosomal peptide production in Saccharomyces cerevisiae
Authors: Siewers Verena , San‐Bento Rita , Nielsen Jens 
Published: March 2010

AbstractSaccharomyces cerevisiae has in several cases been proven to be a suitable host for the production of natural products and was recently exploited for the production of non‐ribosomal peptides. Synthesis of non‐ribosomal peptides (NRPs) is mediated by NRP synthetases (NRPSs), modular enzymes, which are often organized in enzyme complexes. In these complexes, partner NRPSs interact via communication‐mediating domains (COM domains). In order to test whether functional interaction between separate NRPS modules is possible in yeast we constructed a yeast strain expressing two modules with compatible COM domains from two plasmids. Successful production as well as secretion of the expected dipeptide was detected. This opens the possibility of using yeast as a eukaryotic platform for fast assessment of new module combinations for the development of novel NRP compounds. Biotechnol. Bioeng. 2010;106: 841–844. © 2010 Wiley Periodicals, Inc.
Development of a 3D human in vitro skin co‐culture model for detecting irritants in real‐time
Authors: Cantòn I. , Cole D.M. , Kemp E.H. , Watson P.F. , Chunthapong J. , Ryan A.J. , MacNeil S. , Haycock J.W. 
Published: March 2010

AbstractTissue engineered materials for clinical purposes have led to the development of in vitro models as alternatives to animal testing. The aim of this study was to understand the paracrine interactions arising between keratinocytes and fibroblasts for detecting and discriminating between an irritant‐induced inflammatory reaction and cytotoxicy. We used two irritants [sodium dodecyl sulphate (SDS) and potassium diformate (Formi®)] at sub‐toxic concentrations and studied interleukin‐1 alpha (IL‐1α) release from human keratinocytes and activation of NF‐κB in human fibroblasts. NF‐κB activation in fibroblast 2D cultures required soluble factors released by prior incubation of keratinocytes with either SDS or Formi®. Neither cell type responded directly to either agent, confirming a paracrine mechanism. Fibroblasts were then cultured in 3D microfiber scaffolds and transfected with an NF‐κB reporter construct linked to GFP. Findings for 3D cultures were similar to those in 2D in that soluble factors released by prior incubation of keratinocytes with SDS or Formi® was required for NF‐κB activation in fibroblasts. Similarly, direct incubation with either agent did not directly activate NF‐κB. A technical advantage of using transfected cells in 3D was an ability to detect NF‐κB activation in live fibroblasts. To confirm paracrine signaling a twofold increase in IL‐1α was measured in keratinocyte‐conditioned medium after incubation with SDS or Formi®, which correlated with fibroblast NF‐κB activity. In summary, this work has value for developing 3D tissue engineered co‐culture models for the in vitro testing of irritant chemicals at sub‐toxic concentrations, as an alternative to in vivo models. Biotechnol. Bioeng. 2010;106: 794–803. © 2010 Wiley Periodicals, Inc.
Ecosystem cross‐sector collaboration: conceptualizing an adaptive approach to sustainability governance
Authors: Heuer Mark 
Published: March 2010

AbstractThis paper explores the theoretical underpinnings of collaboration and ecosystem management in order to identify the relationships and processes involved in implementing ecosystem management programs through cross‐sector collaboration. Ecosystem management requires a highly adaptive and resilient social–ecological governance approach, which addresses spatiality and temporality issues. In order to explore possible implementation issues with ecosystem management, propositions are developed dealing with adaptive governance, institutional isomorphism and collective action. The paper concludes with a discussion of the theoretic underpinnings involved in implementing ecosystem management through cross‐sector collaborations. Copyright © 2010 John Wiley & Sons, Ltd and ERP Environment.
Drug‐eluting stents in patients with end‐stage renal disease: Meta‐analysis and systematic review of the literature
Authors: Abdel‐Latif Ahmed , Mukherjee Debabrata , Mesgarzadeh Paymon , Ziada Khaled M. 
Published: March 2010

AbstractObjective: The study sought to examine the total weight of evidence regarding the use of drug eluting (DES) and bare metal stents (BMS) in patients with end stage renal disease (ESRD). Background: The potential superiority of DES over BMS in reducing target lesion or vessel revascularization (TLR or TVR) in patients with ESRD on dialysis has not been established. Small studies comparing DES to BMS in this population have yielded inconclusive results mainly due to the small sample size. Methods: We searched MEDLINE, EMBASE, Science Citation Index, CINAHL, and the Cochrane CENTRAL database of controlled clinical trials (December 2009) for controlled trials comparing DES to BMS in ESRD patients. We conducted a fixed‐effects meta‐analysis across seven eligible studies (n = 869 patients). Results: Compared with BMS‐treated patients, DES‐treated patients had significantly lower TLR/TVR (OR 0.55 CI: 0.39–0.79) and major adverse cardiac events (MACE) (OR 0.54; CI: 0.40–0.73). The absolute risk reduction (ARR) with DES in TLR/TVR was −0.09 (CI: −0.14 to −0.04; NNT 11) and in MACE was −0.13 (CI: −0.19 to −0.07; NNT 8). A trend towards lower incidence of all cause mortality was also noted with DES (OR 0.68; CI: 0.45–1.01). No significant differences were noted between both groups in the relative or absolute risk of myocardial infarction. Conclusion: The use of DES in patients with ESRD is safe and yields significant reduction in the risk of TLR/TVR and MACE. Larger randomized studies are needed to confirm the results of this meta‐analysis and establish the appropriate stent choice in this high risk population. © 2010 Wiley‐Liss, Inc.
Swan‐Ganz‐induced pulmonary artery rupture: Management with stent graft implantation
Authors: Zuffi Andrea , Biondi‐Zoccai Giuseppe , Colombo Federico 
Published: March 2010

AbstractPulmonary artery catheterization is a useful tool for the diagnosis and management of lung or cardiac disease. This procedure is considered safe and associated with a low incidence of major complications. However, pulmonary artery rupture during right heart catheterization, albeit rare, remains a severe complication. Despite modern management with metal‐coil embolization, selective intubation and deployment of bronchial blocker, the mortality rate may be as high as 50%. In this case, we report a new approach to deal with a Swan‐Ganz‐induced pulmonary artery rupture based on stent graft implantation leading to successful sealing of the pulmonary perforation with final patency and normal antegrade blood flow in the pulmonary branch. © 2010 Wiley‐Liss, Inc.
Electron Attachment to Hydrated Oligonucleotide Dimers: Guanylyl‐3′,5′‐Cytidine and Cytidylyl‐3′,5′‐Guanosine
Authors: Gu Jiande , Xie Yaoming , Schaefer Henry F. 
Published: March 2010

AbstractThe dinucleoside phosphate deoxycytidylyl‐3′,5′‐deoxyguanosine (dCpdG) and deoxyguanylyl‐3′,5′‐deoxycytidine (dGpdC) systems are among the largest to be studied by reliable theoretical methods. Exploring electron attachment to these subunits of DNA single strands provides significant progress toward definitive predictions of the electron affinities of DNA single strands. The adiabatic electron affinities of the oligonucleotides are found to be sequence dependent. Deoxycytidine (dC) on the 5′ end, dCpdG, has larger adiabatic electron affinity (AEA, 0.90 eV) than dC on the 3′ end of the oligomer (dGpdC, 0.66 eV). The geometric features, molecular orbital analyses, and charge distribution studies for the radical anions of the cytidine‐containing oligonucleotides demonstrate that the excess electron in these anionic systems is dominantly located on the cytosine nucleobase moiety. The π‐stacking interaction between nucleobases G and C seems unlikely to improve the electron‐capturing ability of the oligonucleotide dimers. The influence of the neighboring base on the electron‐capturing ability of cytosine should be attributed to the intensified proton accepting–donating interaction between the bases. The present investigation demonstrates that the vertical detachment energies (VDEs) of the radical anions of the oligonucleotides dGpdC and dCpdG are significantly larger than those of the corresponding nucleotides. Consequently, reactions with low activation barriers, such as those for OC σ bond and N‐glycosidic bond breakage, might be expected for the radical anions of the guanosine–cytosine mixed oligonucleotides.
Organosilyl/‐germyl Polyoxotungstate Hybrids for Covalent Grafting onto Silicon Surfaces: Towards Molecular Memories
Authors: Joo Nicoleta , Renaudineau Séverine , Delapierre Guillaume , Bidan Gérard , Chamoreau Lise‐Marie , Thouvenot René , Gouzerh Pierre , Proust Anna 
Published: March 2010

AbstractOrganosilyl/‐germyl polyoxotungstate hybrids [PW9O34(tBuSiO)3Ge(CH2)2CO2H]3− (1 a), [PW9O34(tBuSiO)3Ge(CH2)2CONHCH2CCH]3− (2 a), [PW11O39Ge(CH2)2CO2H]4− (3 a), and [PW11O39Ge(CH2)2CONHCH2C≡CH]4− (4 a) have been prepared as tetrabutylammonium salts and characterized in solution by multinuclear NMR spectroscopy. The crystal structure of (NBu4)31 a⋅H2O has been determined and the electrochemical behavior of 1 a and 2 a has been investigated by cyclic voltammetry. Covalent grafting of 2 a onto an n‐type silicon wafer has been achieved and the electrochemical behavior of the grafted clusters has been investigated. This represents the first example of covalent grafting of Keggin‐type clusters onto a Si surface and a step towards the realization of POM‐based multilevel memory devices.
Rational Design of Fluorescent Bioimaging Probes by Controlling the Aggregation Behavior of Squaraines: A Special Effect of Ionic Liquid Pendants
Authors: Wang Wenhai , Fu Afu , Lan Jingbo , Gao Ge , You Jingsong , Chen Lijuan 
Published: March 2010

AbstractWe herein present an effective strategy to create water‐soluble fluorescent bioimaging dyes by introducing the imidazolium‐based ionic liquid (IL) pendants into a fluorescent skeleton. A new type of water‐soluble imidazolium‐anchored squaraine dye was synthesized accordingly. The relationship between the aggregate of squaraines and their fluorescent cell imaging application was elucidated in detail. Firstly, the aggregation behavior of squaraines in water solutions could be suppressed by varying the alkyl chain attached to the imidazolium unit. Secondly, the capability of cellular uptake and staining of dyes was also dramatically enhanced upon increasing the length of the paraffinic chain. These squaraine dyes displayed an excellent photostability that could permit real‐time fluorescence bioimaging experiments to be monitored over a long time period with constant sample irradiation. Additionally, we designed for the first time an FeII‐ion probe on the basis of an attack of the hydroxyl radical to the four‐membered ring of squaraine. The results demonstrated that the imidazolium‐anchored squaraines could perform “naked‐eye” detection of the Fe2+ ion over a wide range of other interfering metals in aqueous media. More surprisingly, this process showed a fluorescence “turn‐off” and “‐on” response through the regeneration of squaraines in cells.
Heteroheptacenes with Fused Thiophene and Pyrrole Rings
Authors: Gao Peng , Cho Don , Yang Xiaoyin , Enkelmann Volker , Baumgarten Martin , Müllen Klaus 
Published: March 2010

AbstractThe preparation of conjugated heteroheptacenes using an electrophilic coupling reaction induced by a super acid is reported. The new molecules containing thiophene and/or pyrrole rings are bisbenzo[b,b′]thienodithieno[3,2‐b:2′,3′‐d]pyrrole, bisbenzo[b,b′]thienocyclopenta[2,1‐b:3,4‐b′]dithiophene, and bisthieno[3,2‐b]thieno[2,3‐f:5,4‐f′]carbazole. Dithieno[3,2‐b:2′,3′‐d]pyrrole, cyclopenta[2,1‐b:3,4‐b′]dithiophene, and carbazole are used as the aromatic cores. This versatility provides access to molecules with systematically controllable physicochemical properties. Single‐crystal X‐ray analyses demonstrate that the type and position of the alkyl substituents significantly changes the packing properties of the new molecules. The optical and optoelectronic properties of the heteroheptacenes vary considerably depending on the number and position of the sulfur or nitrogen linkages and reveal the improved environmental stability over their hydrocarbon counterparts. The analysis of the experimental results from UV/Vis absorption/photoluminescence (PL) spectroscopy and cyclic voltammetry were combined with DFT quantum‐chemical calculations and compared with other model heteroheptacenes. The results suggest that among the acenes with the same number of fused rings, the thiophene ring fusion inside the skeleton stabilizes both HOMO and LUMO levels more effectively than pyrrole and benzene rings. The present study also shows that the new heteroheptacenes are promising candidates for the construction of electronic materials.
ChemInform Abstract: KBa7Mg2B14O28F5, a New Borate with an Unusual Heptaborate Group and Double Perovskite Unit.
Authors: Li R. K. , Chen Peng 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Intermolecular Dihydrogen‐ and Hydrogen‐Bonding Interactions in Diammonium closo‐Decahydrodecaborate Sesquihydrate.
Authors: Yisgedu Teshome B. , Chen Xuenian , Lingam Hima K. , Huang Zhenguo , Meyers Edward A. , Shore Sheldon G. , Zhao Ji‐Cheng 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: On the Magnetic and Moessbauer Spectroscopic Characterization of EuPd2Sb2.
Authors: Schellenberg Inga , Eul Matthias , Poettgen Rainer 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: New Ramsdellites LiTi2‐yVyO4 (0 ≤ y ≤ 1): Synthesis, Structure, Magnetic Properties and Electrochemical Performances as Electrode Materials for Lithium Batteries.
Authors: Kuhn Alois , Martin Maria , Garcia‐Alvarado Flaviano 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Nd6Li(BO3)3O4F2: A Novel Neodymium Oxyborate Fluoride Containing Two‐Dimensional Nd Layers.
Authors: Xia M. J. , Li R. K. 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: The Mixed‐Valent Cobalt Lithium Phosphate Li4+xCo2‐x(P2O7)2 (x = 0.03): Structural Study and Analysis of the Charge Distribution.
Authors: Kouass Salah , Guesmi Abderrahmen , Driss Ahmed 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: TaRhGe with TiNiSi‐Type Structure.
Authors: Dinges Tim , Eul Matthias , Poettgen Rainer 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: A New Reaction of 1,2‐Di‐ and 1,2,3‐Trialkyldiaziridines: Ring Expansion under the Action of Diethyl Acetylenedicarboxylate in Ionic Liquids.
Authors: Syroeshkina Yuliya S. , Kuznetsov Vladimir V. , Kachala Vadim V. , Makhova Nina N. 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Thermal Rearrangement of Thiocarbonyl‐Stabilized Triphenylphosphonium Ylides Leading to (Z)‐1‐Diphenylphosphino‐2‐phenylsulfonylalkenes.
Authors: Aitken R. Alan , Dawson Graham , Keddie Neil S. , Kraus Helmut , Slawin Alexandra M. Z. , Wheatley Joanne , Woollins J. Derek 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.
ChemInform Abstract: Solar Light‐Driven Photocatalyzed Alkylations. Chemistry on the Window Ledge.
Authors: Protti Stefano , Ravelli Davide , Fagnoni Maurizio , Albini Angelo 
Published: March 2010

AbstractChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.