bluuebunny/crossref_metadata_2025_split huggingface dataset:
An Outsized Contribution of Rivers to Carbon Emissions From Interconnected Urban River‐Lake Networks Within Plains
Authors: Liu Boyi , Zhang Runyu , Zhu Lin , Wang Jun , Qin Boqiang , Shi Wenqing 
Published: April 2024

AbstractUrban aquatic ecosystems in plains are often subject to extensive anthropogenic pollutant inputs and have prolonged times for pollutant degradation, potentially leading to diverse carbon emission patterns. This study explored carbon emission patterns and underlying mechanisms in Ge Lake and its tributaries, located in an urban area within a plain in China. The results revealed that carbon emissions from rivers were significantly higher than those from the downstream lake. Spatial interpolation analysis further revealed that CO2‐eq emissions from a 1‐km2 river area can be equivalent to those from an area as large as 86‐km2 of the downstream lake. Rivers are the gateway for the entry of organic compounds, often carrying substances that are readily biodegradable. As the river water moves slowly, these compounds accumulate and undergo degradation in rivers before they reach downstream lakes. The findings may benefit the estimates of carbon emissions in these regions with greater precision.
Assessment of Polar Ionospheric Observations by VIPIR/Dynasonde at Jang Bogo Station, Antarctica: 2. Ionospheric Ion Drift Velocity
Authors: Kwon Hyuck‐Jin , Jee Geonhwa , Ham Young‐Bae , Zabotin Nikolay , Lee Changsup , Kim Ensol , Bullett Terence W. 
Published: April 2024

AbstractSince the installation at the Antarctic Jang Bogo Station (JBS) in 2017, Korea Polar Research Institute (KOPRI) has been operating the Vertical Incidence Pulsed Ionospheric Radar (VIPIR) equipped with Dynasonde analysis (JVD). The two‐dimensional ion velocity is one of the key ionospheric parameters obtained from the JVD. The ionospheric ion velocities are compared with simultaneous, but independent, measurements of the Doppler velocity obtained from SuperDARN East radar at Dome C. The JVD ion velocity vector is projected to the line‐of‐sight direction of the SuperDARN observation over the JBS to be directly compared with each other. The result of comparison shows a reasonable agreement with the correlation coefficient of 0.72. The linear regression coefficient of about 0.5 represents that the JVD ion velocity is generally smaller than the SuperDARN observations by the regression coefficient, which may result from the different height ranges of the measurements. It is also found that the correlation coefficient increases with increasing magnetic activity (Kp), which suggests that the small‐scale ionospheric density irregularities tend to move with large‐scale plasma motion that is driven by enhanced plasma convection with increasing Kp.
Influence of sipunculan (peanut worm) activity on orifice formation in scleractinian Heterocyathus for adaptation to soft substrates
Authors: Tokuda Yuki , Kawakita Shuya , Sentoku Asuka , Ezaki Yoichi , Tanaka Naoki , Nagasawa Shotaro , Nakaguchi Kazumitsu , Yamaguchi Shuhei , Kondo Yusuke , Ohtsuka Susumu 
Published: April 2024

AbstractMutualism profoundly affects the morphology and ecological evolution of both hosts and symbionts involved. Heterocyathus is a solitary scleractinian coral that lives on soft substrata, and sipunculan worms live symbiotically in the tube-like cavities (orifice) inside the coral skeletons. This habitat provides protection to the sipunculan worms against predators and—owing to the mobility of the worms—prevents the coral from being buried with sediments. The orifice growth is closely related to the symbiont sipunculan worms; however, this has not been previously elucidated. Here, we clarified the growth process of scleractinian coral orifices and the influence of sipunculan activity on this. The orifices were originally formed by rapid accretion deposits. The coral soft tissue enveloping the growth edge of the orifice repeatedly retreated to the outer side due to direct damage to the soft part and/or excessive stress caused by the rubbing of the sipunculan through locomotion, excretion, and feeding behaviour. This resulted in a toppled-domino microskeletal structure appearance and maintenance of the orifice growth. These outcomes demonstrate the first example of the direct influence of symbionts on the skeletal morphogenesis of scleractinian corals. The mutualism between the two organisms is maintained by the beneficial confrontation in forming orifices.
A phase 3 randomized controlled trial of a COVID-19 recombinant vaccine S-268019-b versus ChAdOx1 nCoV-19 in Japanese adults
Authors: Iwata Satoshi , Pollard Andrew J. , Tada Yukio , Omoto Shinya , Shibata Risa Y. , Igarashi Kenji , Hasegawa Takahiro , Ariyasu Mari , Sonoyama Takuhiro 
Published: April 2024

AbstractWe assessed S-268019-b, a recombinant spike protein vaccine with a squalene-based adjuvant, for superiority in its immunogenicity over ChAdOx1 nCoV-19 vaccine among adults in Japan. In this multicenter, randomized, observer-blinded, phase 3 study, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)–naïve participants (aged ≥ 18 years, without prior infection or vaccination against SARS-CoV-2) were randomized (1:1) to receive either S-268019-b or ChAdOx1 nCoV-19 as two intramuscular injections given 28 days apart. Participants who provided consent for a booster administration received S-268019-b at Day 211. The primary endpoint was SARS-CoV-2 neutralizing antibody (NAb) titer on Day 57; the key secondary endpoint was the seroconversion rate for SARS-CoV-2 NAb titer on Day 57. Other endpoints included anti–SARS-CoV-2 S-protein immunoglobulin (Ig)G antibody titer and safety. The demographic and baseline characteristics were generally comparable between S-268019-b (n = 611) and ChAdOx1 nCoV-19 (n = 610) groups. S-268019-b showed superior immunogenicity over ChAdOx1 nCoV-19, based on their geometric mean titers (GMTs) and GMT ratios of SARS-CoV-2 NAb on Day 57 by cytopathic effect assay (GMT [95% confidence interval {CI}] 19.92 [18.68, 21.23] versus 3.63 [3.41, 3.87]; GMT ratio [95% CI] 5.48 [5.01, 6.00], respectively; two-sided p-values < 0.0001). Additionally, NAb measured using a cell viability assay also showed similar results (GMT [95% CI] 183.25 [168.04, 199.84] versus 24.79 [22.77, 27.00]; GMT ratio [95% CI] 7.39 [6.55, 8.35] for S-268019-b versus ChAdOx1 nCoV-19, respectively; p < 0.0001). The GMT of anti–SARS-CoV-2 S-protein IgG antibody was 370.05 for S-268019-b versus 77.92 for ChAdOx1 nCoV-19 on Day 57 (GMT ratio [95% CI] 4.75 [4.34, 5.20]). Notably, immune responses were durable through the end of the study. S-268019-b elicited T-helper 1 skewed T-cell response, comparable to that of ChAdOx1 nCoV-19. After the first dose, the incidence of solicited systemic treatment-related adverse events (TRAEs) was higher in the ChAdOx1 nCoV-19 group, but after the second dose, the incidence was higher in the S-268019-b group. Headache, fatigue, and myalgia were the most commonly reported solicited systemic TRAEs, while pain at the injection site was the most frequently reported solicited local TRAE following both doses in both groups. No serious treatment-related adverse serious TRAEs events were reported in the two groups. S-268019-b was more immunogenic than ChAdOx1 nCoV-19 vaccine and was well tolerated (jRCT2051210151).
The effect of processing partial information in dynamic face perception
Authors: Alp Nihan , Lale Gülce , Saglam Ceren , Sayim Bilge 
Published: April 2024

AbstractFace perception is a major topic in vision research. Most previous research has concentrated on (holistic) spatial representations of faces, often with static faces as stimuli. However, faces are highly dynamic stimuli containing important temporal information. How sensitive humans are regarding temporal information in dynamic faces is not well understood. Studies investigating temporal information in dynamic faces usually focus on the processing of emotional expressions. However, faces also contain relevant temporal information without any strong emotional expression. To investigate cues that modulate human sensitivity to temporal order, we utilized muted dynamic neutral face videos in two experiments. We varied the orientation of the faces (upright and inverted) and the presence/absence of eye blinks as partial dynamic cues. Participants viewed short, muted, monochromic videos of models vocalizing a widely known text (National Anthem). Videos were played either forward (in the correct temporal order) or backward. Participants were asked to determine the direction of the temporal order for each video, and (at the end of the experiment) whether they had understood the speech. We found that face orientation, and the presence/absence of an eye blink affected sensitivity, criterion (bias) and reaction time: Overall, sensitivity was higher for upright compared to inverted faces, and in the condition where an eye blink was present compared to the condition without an eye blink. Reaction times were mostly faster in the conditions with higher sensitivity. A bias to report inverted faces as ‘backward’ observed in Experiment I, where upright and inverted faces were presented randomly interleaved within each block, was absent when presenting upright and inverted faces in different blocks in Experiment II. Language comprehension results revealed that there was higher sensitivity when understanding the speech compared to not understanding the speech in both experiments. Taken together, our results showed higher sensitivity with upright compared to inverted faces, suggesting that the perception of dynamic, task-relevant information was superior with the canonical orientation of the faces. Furthermore, partial information coming from eye blinks, in addition to mouth movements, seemed to play a significant role in dynamic face perception, both when faces were presented upright and inverted. We suggest that studying the perception of facial dynamics beyond emotional expressions will help us to better understand the mechanisms underlying the temporal integration of facial information from different -partial and holistic- sources, and that our results show how different strategies, depending on the available information, are employed by human observers when judging the temporal order of faces.
Dynamics of charged particles in a solenoid and the accurate determination of the magnetic axis of a solenoid using measured field data
Authors: Sinha Gautam , Malik Ritesh 
Published: April 2024

AbstractA fundamental understanding of the theory and measurement procedures for characterizing a solenoid is crucial for making a project cost-effective and achieving the desired performance for its use in a LINAC. This paper attempts to comprehend the beam dynamics of charged particles by considering the conservation of canonical angular momentum, which can take on positive, negative, and zero values. The analytical expression to generate a parallel beam using a solenoid is derived. The effect of the final beam size on the values of a solenoid’s spherical aberration coefficient and the position of the minimum beam size with respect to the focal length is studied using electron trajectories. A practical procedure to tune the above two parameters of a solenoid is demonstrated by simply modifying the demountable disks connected at each end. A mathematical model is proposed to accurately determine the axis of a solenoid and the magnetic centre using the measured magnetic field data, which is validated experimentally. Several solenoids, used in the 9.5 MeV 10 kW electron LINAC suitable for sterilizing medical products and food irradiation, have been characterised using this procedure.
Targeted potent antimicrobial and antitumor oxygen-heterocyclic-based pyran analogues: synthesis and computational studies
Authors: El-Wahab Ashraf H. F. Abd , Borik Rita M. , Al-Dies Al-Anood M. , Fouda Ahmed M. , Mohamed Hany M. , El-Eisawy Raafat A. , Sharaf Mohamed H. , Alzahrani Abdullah Y. A. , Elhenawy Ahmed A. , El-Agrody Ahmed M. 
Published: April 2024

AbstractThe process of creating a series of 3-amino-1-aryl-8-methoxy-1H-benzo[f]chromene-2-carbonitriles (4a-q) involved reacting 6-methoxynaphthalen-2-ol (1), the appropriate aromatic aldehydes (2a-q), and malononitrile (3) in an absolute ethanol/piperidine solution under Ultrasonic irradiation. However, the attempt to create 3-amino-1-aryl-1H-benzo[f]chromene-2,8-dicarbonitrile (6a, d, e) was unsuccessful when 6-cyanonaphthalen-2-ol (5) was stirred at room temperature, reflux, Microwave irradiation, or Ultrasonic irradiation. In addition, the target molecules were screened against Staphylococcus aureus (MRSA), Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Escherichia coli and Klebsiella pneumonia, as well as a panel of three human cancer cells lines such as MCF-7, HCT-116, HepG-2 and two normal cell lines HFL-1 and WI-38. The obtained results confirmed that the pyran derivatives (4 m, i, k) which have a double chlorine at 3,4/2,3/2,5-positions, a single halogen atom 3-Cl/4-Br (4c, e) and a double bromine at 3,5-positions with a single methoxy group at 2-position (4n), of phenyl ring, and, to a lesser extent, other pyran derivatives with monoihalogenated (4a, b, d, f), dihalogenated (4 g, h, j, l) or trisubstituent phenyl ring (4o, p, q). Furthermore, compounds 4b-e, g, i, j, m, and n showed negligible activity against the two normal cell lines, HFL-1 and WI-38. Moreover, compound 4 g exhibited the strongest antimicrobial activity among the other pyran derivatives (4a-f, g-q) when compared to Ciprofloxacin. The MIC was assessed and screened for compound 4 g, revealing bactericidal effects. Lastly, SAR and molecular docking were studied.
Green synthesis and characterization of binary, ternary, and quaternary Ti/MMO anodes for chlorine and oxygen evolution reactions
Authors: Abdel-Aziz A. B. , Heakal F. El-Taib , El Nashar R. M. , Ghayad I. M. 
Published: April 2024

AbstractDimensionally stable anodes of titanium (Ti) metal coated with mixed metal oxides (MMO) are widely used in several electrochemical applications, especially chloro-alkali electrolysis. Herein, we deposited MMO coatings on Ti substrates in different compositions, namely, (60%RuO2-40%TiO2), (60%RuO2-30%TiO2-10%IrO2), and (60%RuO2-20%TiO2-15%IrO2-5%Ta2O5), where RuO2 has the same percentage ratio in all coatings. The aim was to use these electrodes for chlorine evolution reaction (CER) and oxygen evolution reaction (OER) applications. Electrochemical characterization of the coated samples was performed to identify the best Ti/MMO electrodes with the highest efficiencies among the various prepared combinations. The role of IrO2 and Ta2O5 in enhancing corrosion resistance and electrochemical efficacy was up for debate. Scanning electron microscope (SEM), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), and X-ray photoelectron spectroscopy (XPS) analyses were exploited to determine the surface morphology, chemical composition, crystallinity, surface composition, and chemical states of the acquired coatings. The differential scanning calorimetry (DSC) method was used to evaluate the apparent activation energy ($${{\text{E}}}_{{\text{a}}}$$

E
a

) of the deposited MMO. Additionally, the electrochemical performance of our designed coatings was scrutinized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), a current on–off test, a CV stability test (ST), and an accelerated stability test (AST). Furthermore, linear sweep voltammetry (LSV) was incorporated to assess the catalytic efficacy of the prepared anodes toward the CER in a brine solution of pH 2 and the OER in 1 M H2SO4. It became clear that the CER and OER incurred almost the same potential value (1.1 V) on both Ti/RuO2-TiO2 and Ti/RuO2-TiO2-IrO2 electrodes. However, on the Ti/RuO2-TiO2-IrO2-Ta2O5 anode, there was a 0.2 V potential difference between the CER occurring at 1.1 V and the OER happening at 1.3 V.
Comparative study on clinical outcomes in autologous chondrocyte implantation using three-dimensional cultured JACC® with collagen versus periosteum coverings
Authors: Kato Yuki , Yamada Shin , Takazawa Shuzo , Hattori Soichi , Okada Takuya , Ohuchi Hiroshi 
Published: April 2024

AbstractThis study investigates the efficacy of a collagen membrane as a substitute for autologous periosteum in atelocollagen-assisted autologous chondrocyte implantation (ACI) using J-TEC autologous cultured cartilage (JACC®). Sixty-nine patients with knee joint chondral defects underwent ACI using JACC®—34 with periosteum-covered ACI (P-ACIs) and 35 with collagen-covered ACI (C-ACIs). Clinical outcomes were compared through patient-reported measures, International Cartilage Repair Society (ICRS) Cartilage Repair Assessment (CRA) scores at second-look arthroscopy one year postoperatively, and adverse event incidence. Postoperative subjective scores significantly improved up to two years, with no significant differences between P-ACI and C-ACI groups. However, C-ACI exhibited a lower adverse event rate (p = 0.034) and significantly higher ICRS CRA scores (p = 0.0001). Notably, C-ACI outperformed P-ACI in both femoral condyle and trochlea assessments (p = 0.0157 and 0.0005, respectively). While clinical outcomes were comparable, the use of a collagen membrane demonstrated superiority in ICRS CRA during second-look arthroscopy and adverse event occurrence.
Genotypic analyses of IncHI2 plasmids from enteric bacteria
Authors: Algarni Suad , Gudeta Dereje D. , Han Jing , Nayak Rajesh , Foley Steven L. 
Published: April 2024

AbstractIncompatibility (Inc) HI2 plasmids are large (typically > 200 kb), transmissible plasmids that encode antimicrobial resistance (AMR), heavy metal resistance (HMR) and disinfectants/biocide resistance (DBR). To better understand the distribution and diversity of resistance-encoding genes among IncHI2 plasmids, computational approaches were used to evaluate resistance and transfer-associated genes among the plasmids. Complete IncHI2 plasmid (N = 667) sequences were extracted from GenBank and analyzed using AMRFinderPlus, IntegronFinder and Plasmid Transfer Factor database. The most common IncHI2-carrying genera included Enterobacter (N = 209), Escherichia (N = 208), and Salmonella (N = 204). Resistance genes distribution was diverse, with plasmids from Escherichia and Salmonella showing general similarity in comparison to Enterobacter and other taxa, which grouped together. Plasmids from Enterobacter and other taxa had a higher prevalence of multiple mercury resistance genes and arsenic resistance gene, arsC, compared to Escherichia and Salmonella. For sulfonamide resistance, sul1 was more common among Enterobacter and other taxa, compared to sul2 and sul3 for Escherichia and Salmonella. Similar gene diversity trends were also observed for tetracyclines, quinolones, β-lactams, and colistin. Over 99% of plasmids carried at least 25 IncHI2-associated conjugal transfer genes. These findings highlight the diversity and dissemination potential for resistance across different enteric bacteria and value of computational-based approaches for the resistance-gene assessment.
Study of biopolymer encapsulated Eu doped Fe3O4 nanoparticles for magnetic hyperthermia application
Authors: Hazarika Krishna Priya , Borah J. P. 
Published: April 2024

AbstractAn exciting prospect in the field of magnetic fluid hyperthermia (MFH) has been the integration of noble rare earth elements (Eu) with biopolymers (chitosan/dextran) that have optimum structures to tune specific effects on magnetic nanoparticles (NPs). However, the heating efficiency of MNPs is primarily influenced by their magnetization, size distribution, magnetic anisotropy, dipolar interaction, amplitude, and frequency of the applied field, the MNPs with high heating efficiency are still challenging. In this study, a comprehensive experimental analysis has been conducted on single-domain magnetic nanoparticles (SDMNPs) for evaluating effective anisotropy, assessing the impact of particle-intrinsic factors and experimental conditions on self-heating efficiency in both noninteracting and interacting systems, with a particular focus on the dipolar interaction effect. The study successfully reconciles conflicting findings on the interaction effects in the agglomeration and less agglomerated arrangements for MFH applications. The results suggest that effective control of dipolar interactions can be achieved by encapsulating Chitosan/Dextran in the synthesized MNPs. The lower dipolar interactions successfully tune the self-heating efficiency and hold promise as potential candidates for MFH applications.
Quantitative phase imaging by gradient retardance optical microscopy
Authors: Zhang Jinming , Sarollahi Mirsaeid , Luckhart Shirley , Harrison Maria J. , Vasdekis Andreas E. 
Published: April 2024

AbstractQuantitative phase imaging (QPI) has become a vital tool in bioimaging, offering precise measurements of wavefront distortion and, thus, of key cellular metabolism metrics, such as dry mass and density. However, only a few QPI applications have been demonstrated in optically thick specimens, where scattering increases background and reduces contrast. Building upon the concept of structured illumination interferometry, we introduce Gradient Retardance Optical Microscopy (GROM) for QPI of both thin and thick samples. GROM transforms any standard Differential Interference Contrast (DIC) microscope into a QPI platform by incorporating a liquid crystal retarder into the illumination path, enabling independent phase-shifting of the DIC microscope's sheared beams. GROM greatly simplifies related configurations, reduces costs, and eradicates energy losses in parallel imaging modalities, such as fluorescence. We successfully tested GROM on a diverse range of specimens, from microbes and red blood cells to optically thick (~ 300 μm) plant roots without fixation or clearing.
Genome-wide association study of metabolic dysfunction-associated fatty liver disease in a Korean population
Authors: Lee Young , Cho Eun Ju , Choe Eun Kyung , Kwak Min-Sun , Yang Jong In , Oh Seung-Won , Yim Jeong Yoon , Chung Goh Eun 
Published: April 2024

AbstractGenome-wide association studies have identified several genetic variants associated with nonalcoholic fatty liver disease. To emphasize metabolic abnormalities in fatty liver, metabolic (dysfunction)-associated fatty liver disease (MAFLD) has been introduced; thus, we aimed to investigate single-nucleotide polymorphisms related to MAFLD and its subtypes. A genome-wide association study was performed to identify genetic factors related to MAFLD. We used a Korean population-based sample of 2282 subjects with MAFLD and a control group of 4669. We replicated the results in a validation sample which included 639 patients with MAFLD and 1578 controls. Additionally, we categorized participants into three groups, no MAFLD, metabolic dysfunction (MD)-MAFLD, and overweight/obese-MAFLD. After adjusting for age, sex, and principal component scores, rs738409 [risk allele G] and rs3810622 [risk allele T], located in the PNPLA3 gene, showed significant associations with MAFLD (P-values, discovery set = 1.60 × 10–15 and 4.84 × 10–10; odds ratios, 1.365 and 1.284, validation set = 1.39 × 10–4, and 7.15 × 10–4, odds ratios, 1.299 and 1.264, respectively). An additional SNP rs59148799 [risk allele G] located in the GATAD2A gene showed a significant association with MAFLD (P-values, discovery set = 2.08 × 10–8 and validation set = 0.034, odds ratios, 1.387 and 1.250). rs738409 was significantly associated with MAFLD subtypes ([overweight/obese-MAFLD; odds ratio (95% confidence interval), P-values, 1.515 (1.351–1.700), 1.43 × 10–12 and MD-MAFLD: 1.300 (1.191–1.416), 2.90 × 10–9]. There was a significant relationship between rs3810622 and overweight/obese-MAFLD and MD-MAFLD [odds ratios (95% confidence interval), P-values, 1.418 (1.258, 1.600), 1.21 × 10–8 and 1.225 (1.122, 1.340), 7.06 × 10–6, respectively]; the statistical significance remained in the validation set. PNPLA3 was significantly associated with MAFLD and MAFLD subtypes in the Korean population. These results indicate that genetic factors play an important role in the pathogenesis of MAFLD.