bluuebunny/crossref_metadata_2025_split huggingface dataset:
Randomized, controlled trial of telcagepant over four migraine attacks
Authors: Ho Andrew P , Dahlöf Carl GH , Silberstein Stephen D , Saper Joel R , Ashina Messoud , Kost James T , Froman Samar , Leibensperger Heather , Lines Christopher R , Ho Tony W 
Published: June 2010

Methods: This study evaluated the calcitonin gene-related peptide (CGRP) receptor antagonist telcagepant (tablet formulation) for treatment of a migraine attack and across four attacks. Adults with migraine were randomized, double-blind, to telcagepant 140 mg, telcagepant 280 mg, or control treatment sequences to treat four moderate-to-severe migraine attacks. Control patients received placebo for three attacks and telcagepant 140 mg for one attack. Efficacy for the first attack (Attack 1) and consistency of efficacy over multiple attacks were assessed. For an individual patient, consistent efficacy was defined as ≥3 successes, and lack of consistent efficacy was defined as ≥2 failures, in treatment response. A total of 1677 patients treated ≥1 attack and 1263 treated all four attacks. Results: Based on Attack 1 data, telcagepant 140 mg and 280 mg were significantly ( p < .001) more effective than placebo for 2-hour pain freedom, 2-hour pain relief, 2-hour absence of migraine-associated symptoms (phonophobia, photophobia, nausea), and 2–24 hours sustained pain freedom. The percentage of patients with 2-hour pain freedom consistency and 2-hour pain relief consistency was significantly ( p < .001) higher for both telcagepant treatment sequences versus control. Adverse events within 48 hours for telcagepant with an incidence ≥2% and twice that of placebo were somnolence (placebo = 2.3%, 140 mg = 5.9%, 280 mg = 5.7%) and vomiting (placebo = 1.4%, 140 mg = 1.0%, 280 mg = 2.9%). Conclusion: Telcagepant 140 mg and 280 mg were effective for treatment of a migraine attack and were more consistently effective than control for intermittent treatment of up to four migraine attacks. Telcagepant was generally well tolerated. (Clinicaltrials.gov; NCT00483704)
Psychosocial headache measures in Caucasian and African American headache patients: Psychometric attributes and measurement invariance
Authors: Heckman Bernadette Davantes , Berlin Kristoffer S , Watakakosol Rewadee , Pierre Valessa St. 
Published: June 2010

Introduction: As research increasingly examines differences in headache characteristics between Caucasian and African American headache patients, it is requisite to determine the equivalence of existing headache measures for both racial groups. Methods: This study examined the psychometric properties and measurement invariance of four widely used psychosocial headache measures. Caucasians ( N = 173) and African Americans ( N = 114) receiving treatment in four headache subspecialty clinics throughout Ohio completed the Headache Disability Inventory (HDI), the Migraine-Specific Quality of Life (MSQL) measure, the Headache Management Self-Efficacy (HMSE) scale, and the Headache-Specific Locus of Control (HSLC) scale prior to initiating new preventive therapies. Conclusion: All measures demonstrated good internal consistency and measurement invariance. Despite adequate measurement invariance, the recommended factor structure of the HMSE scale yielded a poor fit for both African American and Caucasian patients. With perhaps the exception of the HMSE scale, the HDI, MSQL scale and HSLC scale are psychometrically sound and can be used with both Caucasian and African American headache patients.
Partitioning of trace elements between garnet, clinopyroxene and diamond-forming carbonate-silicate melt at 7 GPa
Authors: Kuzyura A. V. , Wall F. , Jeffries T. , Litvin Yu. A. 
Published: June 2010

AbstractConcentrations of trace elements in coexisting garnet, clinopyroxene and completely miscible carbonate-silicate melt (formed at 7 GPa from the Chagatai silicocarbonatite rock known to be diamondiferous) were determined using LA-ICP-MS. The partition coefficients for Li, Rb, Cs, Ba, Th, U, Ta, Nb, La, Ce, Pb, Pr, Sr, Nd, Zr, Hf, Sm, Eu, Gd, Tb, Dy, Y, Ho, Er, Tm, Yb, Lu, Sc and Zn were determined. The new experimental data for trace-element partitioning between garnet, clinopyroxene and carbonate-silicate melt have been compared with published data for partitioning between garnet, clinopyroxene and carbonatite melt, and garnet, clinopyroxene and silicate melt. The results show that the trace-element partitioning is not significantly altered by changes in melt composition, with HREE always concentrated in the garnet. Carbonate-silicate melt, as a diamond-forming medium, and carbonatite or silicate melt equilibrated with mantle silicate minerals, behave similarly in respect of trace-element distribution.
Arisite-(La), a new REE-fluorcarbonate mineral from the Aris phonolite (Namibia), with descriptions of the crystal structures of arisite-(La) and arisite-(Ce)
Authors: Piilonen P. C. , McDonald A. M. , Grice J. D. , Cooper M. A. , Kolitsch U. , Rowe R. , Gault R. A. , Poirier G. 
Published: June 2010

AbstractArisite-(La), ideally NaLa2(CO3)2[F2x(CO3)1–x]F, is a new layered REE-fluorcarbonate mineral from miarolitic cavities within the Aris phonolite, Namibia (IMA no. 2009-019). It occurs as distinct chemical zones mixed with its Ce-analogue, arisite-(Ce). Crystals are vitreous, transparent beige, beige-yellow, light lemon-yellow to pinkish, and occur as tabular prisms up to 1.5 mm. Arisite-(La) is brittle, has conchoidal fracture, poor cleavage perpendicular to (001), a Mohs hardness of ~3–3½, is not fluorescent in either long- or shortwave UV radiation, dissolves slowly in dilute HCl at room temperature and sinks in methylene iodide, Dcalc. = 4.072 g cm–3. Arisite-(La) is uniaxial negative, has sharp extinction, with both ω and ε exhibiting a range of values within each grain: ω = 1.696–1.717(4) and ε = 1.594–1.611(3), a result of chemical zoning attributed to both Ce ⇌ La and Na ⇌ Ca substitutions. The crystal structure of both arisite-(Ce) and arisite-(La) were solved by direct methods and refined to R = 1.66%, wR2 = 4.31% (Ce) and R = 2.09%, wR2 = 5.26% (La), respectively. Arisite is hexagonal, Pm2, Z = 1, with unit-cell parameters of a = 5.1109(2) Å, c = 8.6713(4) Å, V = 196.16(6) Å3 for arisite-(Ce), and a = 5.1131(7) Å, c = 8.6759(17) Å, V = 196.43(5) Å3 for arisite-(La). Arisite-(Ce) and arisite-(La) are members of the layered, flat-lying REE-fluorcarbonate group which have crystal structures characterized by separate layers of triangular planar groups that parallel the overall layering of the structure, F, REE and alkali or alkaline-earthelements. Overall, the arisite structure can be defined by three distinct layers which parallel (001): (1) ∞[REE(CO3)2F] slabs, (2) sheets of Naϕ9 polyhedra, and (3) ∞[2F/CO3]2–. Based on its (M+F)/C ratio, arisite can further be described as having a dense, flat-lying fluorcarbonate structure, a classification which includes the structurally related mineral species cordylite, kukharenkoite, cebaite, lukechangite, huanghoite, and one incompletely characterized synthetic phase, NaY2(CO3)3F.
Distribution of gallium between phenocrysts and melt in peralkaline salic volcanic rocks, Kenya Rift Valley
Authors: Macdonald R. , Rogers N. W. , Bagiński B. , Dzierżanowski P. 
Published: June 2010

AbstractGallium abundances, determined by laser ablation-inductively coupled plasma-mass spectrometry, are presented for phenocrysts and glassy matrices from a metaluminous trachyte and five peralkaline rhyolites from the Greater Olkaria Volcanic Complex, Kenya Rift Valley. Abundances in the glasses range from 28.9 to 33.3 ppm, comparable with peralkaline rhyolites elsewhere. Phenocryst Ga abundances (in ppm) are: sanidine 31.5–45.3; fayalite 0.02–0.22; hedenbergite 3.3–6.3; amphibole 12; biotite 72; ilmenite 0.56–0.72; titanomagnetite 32; chevkinite-(Ce) 364. The mafic phases and chevkinite-(Ce) are enriched in Ga relative to Al, whereas Ga/Al ratios in sanidine are smaller than in coexisting glass. Apparent partition coefficients range from <0.01 in fayalite to 12 in chevkinite-(Ce). Coefficients for hedenbergite, ilmenite and titanomagnetite decrease as melts become peralkaline. The sharp increase in Ga/Al in the more fractionated members of alkaline magmatic suites probably results from alkali feldspar-dominated fractionation. Case studies are presented to show that the Ga/Al ratio may be a sensitive indicator of such petrogenetic processes as magma mixing, interaction of melts with F-rich volatile phases, mineral accumulation and volatile-induced crustal anatexis.
Tracing the origins and signatures of selection of antifolate resistance in island populations of Plasmodium falciparum
Authors: Salgueiro Patrícia , Vicente José L , Ferreira Conceição , Teófilo Vânia , Galvão André , do Rosário Virgílio E , Cravo Pedro , Pinto João 
Published: June 2010

Abstract

Background
Resistance of the malaria parasite Plasmodium falciparum to sulfadoxine-pyrimethamine (SP) has evolved worldwide. In the archipelago of São Tomé and Principe (STP), West Africa, although SP resistance is highly prevalent the drug is still in use in particular circumstances. To address the evolutionary origins of SP resistance in these islands, we genotyped point mutations at P. falciparum dhfr and dhps genes and analysed microsatellites flanking those genes.

Methods
Blood samples were collected in July and December 2004 in three localities of São Tomé Island and one in Principe Island. Species-specific nested-PCR was used to identify P. falciparum infected samples. Subsequently, SNPs at the dhfr and dhps genes were identified through PCR-RFLP. Isolates were also analysed for three microsatellite loci flanking the dhfr gene, three loci flanking dhps and four loci located at putative neutral genomic regions.

Results
An increase of resistance-associated mutations at dhfr and dhps was observed, in particular for the dhfr/dhps quintuple mutant, associated with clinical SP failure. Analysis of flanking microsatellites suggests multiple independent introductions for dhfr and dhps mutant haplotypes, possibly from West Africa. A reduced genetic diversity and increased differentiation at flanking microsatellites when compared to neutral loci is consistent with a selective sweep for resistant alleles at both loci.

Conclusions
This study provides additional evidence for the crucial role of gene flow and drug selective pressures in the rapid spread of SP resistance in P. falciparum populations, from only a few mutation events giving rise to resistance-associated mutants. It also highlights the importance of human migration in the spread of drug resistant malaria parasites, as the distance between the islands and mainland is not consistent with mosquito-mediated parasite dispersal.
Two heterologously expressed Planobispora rosea proteins cooperatively induce Streptomyces lividans thiostrepton uptake and storage from the extracellular medium
Authors: Giardina Anna , Alduina Rosa , Gottardi Elvira , Di Caro Valentina , Süssmuth Roderich D , Puglia Anna M 
Published: June 2010

Abstract

Background
A bacterial artificial chromosomal library of Planobispora rosea, a genetically intractable actinomycete strain, was constructed using Escherichia coli-Streptomyces artificial chromosome (ESAC) and screened for the presence of genes known to be involved in the biosynthesis of antibiotics.

Results
One clone with a 40 kb insert showed antimicrobial activity against Gram positive bacteria. Insert sequence analysis and subcloning experiments revealed that the bioactivity was due to a 3.5 kb DNA fragment containing two open reading frames. These orfs encode two proteins with high similarity to a putative membrane protein of Streptomyces coelicolor and to the nogalamycin resistance protein SnorO of Streptomyces nogalater, respectively. The role of these two Orfs is unknown in Planobispora. Disruption and complementation experiments revealed that both proteins are necessary for the antibacterial activity and chemical analysis demonstrated that the antibiotic activity was due to thiostrepton, antibiotic used as recombinant clone selection marker.

Conclusion
Two Planobispora rosea orfs are responsible for increasing intracellular amounts and storage of thiostrepton in Streptomyces lividans.
Naturally occurring nanoparticles from English ivy: an alternative to metal-based nanoparticles for UV protection
Authors: Xia Lijin , Lenaghan Scott C , Zhang Mingjun , Zhang Zhili , Li Quanshui 
Published: June 2010

Abstract

Background
Over the last decade safety concerns have arisen about the use of metal-based nanoparticles in the cosmetics field. Metal-based nanoparticles have been linked to both environmental and animal toxicity in a variety of studies. Perhaps the greatest concern involves the large amounts of TiO2 nanoparticles that are used in commercial sunscreens. As an alternative to using these potentially hazardous metal-based nanoparticles, we have isolated organic nanoparticles from English ivy (Hedera helix). In this study, ivy nanoparticles were evaluated for their potential use in sunscreens based on four criteria: 1) ability to absorb and scatter ultraviolet light, 2) toxicity to mammalian cells, 3) biodegradability, and 4) potential for diffusion through skin.

Results
Purified ivy nanoparticles were first tested for their UV protective effects using a standard spectrophotometric assay. Next the cell toxicity of the ivy nanoparticles was compared to TiO2 nanoparticles using HeLa cells. The biodegradability of these nanoparticles was also determined through several digestion techniques. Finally, a mathematical model was developed to determine the potential for ivy nanoparticles to penetrate through human skin. The results indicated that the ivy nanoparticles were more efficient in blocking UV light, less toxic to mammalian cells, easily biodegradable, and had a limited potential to penetrate through human skin. When compared to TiO2 nanoparticles, the ivy nanoparticles showed decreased cell toxicity, and were easily degradable, indicating that they provided a safer alternative to these nanoparticles.

Conclusions
With the data collected from this study, we have demonstrated the great potential of ivy nanoparticles as a sunscreen protective agent, and their increased safety over commonly used metal oxide nanoparticles.
CyberKnife® enhanced conventionally fractionated chemoradiation for high grade glioma in close proximity to critical structures
Authors: Oermann Eric , Collins Brian T , Erickson Kelly T , Yu Xia , Lei Sue , Suy Simeng , Hanscom Heather N , Kim Joy , Park Hyeon U , Eldabh Andrew , Kalhorn Christopher , McGrail Kevin , Subramaniam Deepa , Jean Walter C , Collins Sean P 
Published: June 2010

Abstract

Introduction
With conventional radiation technique alone, it is difficult to deliver radical treatment (≥ 60 Gy) to gliomas that are close to critical structures without incurring the risk of late radiation induced complications. Temozolomide-related improvements in high-grade glioma survival have placed a higher premium on optimal radiation therapy delivery. We investigated the safety and efficacy of utilizing highly conformal and precise CyberKnife radiotherapy to enhance conventional radiotherapy in the treatment of high grade glioma.

Methods
Between January 2002 and January 2009, 24 patients with good performance status and high-grade gliomas in close proximity to critical structures (i.e. eyes, optic nerves, optic chiasm and brainstem) were treated with the CyberKnife. All patients received conventional radiation therapy following tumor resection, with a median dose of 50 Gy (range: 40 - 50.4 Gy). Subsequently, an additional dose of 10 Gy was delivered in 5 successive 2 Gy daily fractions utilizing the CyberKnife® image-guided radiosurgical system. The majority of patients (88%) received concurrent and/or adjuvant Temozolmide.

Results
During CyberKnife treatments, the mean number of radiation beams utilized was 173 and the mean number of verification images was 58. Among the 24 patients, the mean clinical treatment volume was 174 cc, the mean prescription isodose line was 73% and the mean percent target coverage was 94%. At a median follow-up of 23 months for the glioblastoma multiforme cohort, the median survival was 18 months and the two-year survival rate was 37%. At a median follow-up of 63 months for the anaplastic glioma cohort, the median survival has not been reached and the 4-year survival rate was 71%. There have been no severe late complications referable to this radiation regimen in these patients.

Conclusion
We utilized fractionated CyberKnife radiotherapy as an adjunct to conventional radiation to improve the targeting accuracy of high-grade glioma radiation treatment. This technique was safe, effective and allowed for optimal dose-delivery in our patients. The value of image-guided radiation therapy for the treatment of high-grade gliomas deserves further study.
Obesity affects the chondrocyte responsiveness to leptin in patients with osteoarthritis
Authors: Pallu Stéphane , Francin Pierre-Jean , Guillaume Cécile , Gegout-Pottie Pascale , Netter Patrick , Mainard Didier , Terlain Bernard , Presle Nathalie 
Published: June 2010

Abstract
Introduction
Increasing evidence support the regulatory role of leptin in osteoarthritis (OA). As high circulating concentrations of leptin disrupt the physiological function of the adipokine in obese individuals, the current study has been undertaken to determine whether the elevated levels of leptin found in the joint from obese OA patients also induce changes in the chondrocyte response to leptin.

Methods
Chondrocytes isolated from OA patients with various body mass index (BMI) were treated with 20, 100 or 500 ng/ml of leptin. The expression of cartilage-specific components (aggrecan, type 2 collagen), as well as regulatory (IGF-1, TGFβ, MMP-13, TIMP 2) or inflammatory (COX-2, iNOS, IL-1) factors was investigated by real-time PCR to evaluate chondrocyte responsiveness to leptin. Furthermore, the effect of body mass index (BMI) on leptin signalling pathways was analyzed with an enzyme-linked immunosorbent assay for STATs activation.

Results
Leptin at 20 ng/ml was unable to modulate gene expression in chondrocytes, except for MMP-13 in obese OA patients. Higher leptin levels induced the expression of IGF-1, type 2 collagen, TIMP-2 and MMP-13. However, the activity of the adipokine was shown to be critically dependent on both the concentration and the BMI of the patients with a negative association between the activation of regulated genes and BMI for 100 ng/ml of adipokine, but a positive association between chondrocyte responsiveness and BMI for the highest leptin dose. In addition, the gene encoding MMP-13 was identified as a target of leptin for chondrocytes originated from obese patients while mRNA level of TIMP-2 was increased in leptin-treated chondrocytes collected from normal or overweight patients. The adipokine at 500 ng/ml triggered signal transduction through a STAT-dependent pathway while 100 ng/ml of leptin failed to activate STAT 3 but induced STAT 1α phosphorylation in chondrocytes obtained from obese patients.

Conclusions
The current study clearly showed that characteristics of OA patients and more expecially obesity may affect the responsiveness of cultured chondrocytes to leptin. In addition, the BMI-dependent effect of leptin for the expression of TIMP-2 and MMP-13 may explain why obesity is associated with an increased risk for OA.
Glucagon-Like Peptide-1 Analog and Insulin Combination Therapy in the Management of Adults with Type 2 Diabetes Mellitus
Authors: Tzefos Maria , Olin Jacqueline L 
Published: June 2010

Objective: To evaluate the efficacy and tolerability of combination glucagon-like peptide-1 (GLP-1) analogs and insulin in the management of type 2 diabetes mellitus (T2DM) in adults. Data Source: A MEDLINE search (1966–April 2010) was conducted using the key terms glucagon-like peptide-1 analog, exenatide, incretin mimetic, liraglutide, diabetes mellitus, and insulin. Study Selection and Data Extraction: All English-language articles identified from the data source were evaluated and reviewed for inclusion. Original research and retrospective cohorts were included in this review. The references of articles that we identified were examined for any additional studies appropriate for review. Data Synthesis: Exenatide is a subcutaneously administered GLP-1 receptor agonist that is used for the improvement of glycemic control in adults with T2DM. Through actions similar to those of endogenous GLP-1, exenatide contributes to improved postprandial glycemic control and weight loss. The concomitant use of exenatide and insulin is currently not Food and Drug Administration-approved due to lack of clinical trial data. However, combination insulin and exenatide may be advantageous, especially for reducing weight gain, particularly for obese patients with T2DM. Several small prospective and retrospective studies evaluating combination therapy found statistically significant reductions in hemoglobin A1c (A1C), weight, and total daily insulin dose requirements. The most common adverse effects reported included gastrointestinal effects, such as nausea and vomiting, and hypoglycemia. Conclusions: Although there is a limited amount of data and not all studies demonstrated A1C reduction, the combination of exenatide with insulin therapy appears to be a safe option in the management of T2DM. It may be a promising therapeutic strategy for some patients, as reductions in weight and insulin doses were observed. Further well-designed prospective trials are warranted to fully determine the long-term effectiveness and safety of this combination as well as its place in therapy.
A Multiplex Immunoassay for Human Adipokine Profiling
Authors: Schipper Henk S , de Jager Wilco , van Dijk Mariska EA , Meerding Jenny , Zelissen Pierre MJ , Adan Roger A , Prakken Berent J , Kalkhoven Eric 
Published: June 2010

BACKGROUNDAdipose tissue secretory proteins, called adipokines, play pivotal roles in the pathophysiology of obesity and its associated disorders such as metabolic syndrome, type 2 diabetes, and cardiovascular disease. Because methods for comprehensive adipokine profiling in patient plasma and other biological samples are currently limited, we developed a multiplex immunoassay for rapid and high-throughput measurement of 25 adipokines in only 50 μL of sample.METHODS(Pre)adipocyte and ex vivo cultured adipose tissue supernatants were generated and together with plasma from 5 morbidly obese patients and 5 healthy and normal weight controls used to develop the adipokine multiplex immunoassay and test its usefulness in biological samples. We assessed adipokine dynamic ranges, lower limits of detection and quantification, cross-reactivity, intra- and interassay variation, and correlation with adipokine ELISAs.RESULTSThe limits of quantification and broad dynamic ranges enabled measurement of all 25 adipokines in supernatants and patient plasmas, with the exception of TNF-α in plasma samples. Intraassay variation was &lt;10% for all adipokines; interassay variation was &lt;15%. The multiplex immunoassay results correlated significantly with ELISA measurements. Plasma adipokine profiling showed significantly higher concentrations of the novel adipokines cathepsin S (5.1 × 104 vs 4.3 × 104 ng/L, P = 0.003) and chemerin (4.1 × 105 vs 2.7 × 105 ng/L, P = 0.0008) in morbidly obese patients than normal weight controls, besides the established differences in adiponectin and leptin concentrations.CONCLUSIONSOur findings underscore the relevance of the novel adipokines cathepsin S and chemerin, but foremost the potential of this novel method for both comprehensive adipokine profiling in large patient cohorts and for biological discovery.