bluuebunny/crossref_metadata_2025_split huggingface dataset:
Serodiagnosis of Chlamydia pneumoniae infection using three inclusion membrane proteins
Authors: Hongliang Chen , Zhou Zhou , Zhan Hu , Yanhua Zeng , Zhongyu Li , Yingbiao Lin , Guozhi Dai , Yimou Wu 
Published: January 2010

AbstractThe Chlamydia pneumoniae genome‐encoded open reading frames Cpn0146, Cpn0147, and Cpn0308 were expressed as recombinant proteins for detecting C. pneumoniae‐specific antibodies in samples from three groups of individuals including 183 with C. pneumoniae‐associated respiratory infection (group I), 60 healthy blood donors (group II), and 32 with no known respiratory infection (group III). The recombinant Cpn0146 was recognized by 71 (38.8% positive recognition rate), 15 (25%) and 1 (3.1%), Cpn0147 by 75 (40.9%), 14 (23.3%), and 2 (6.3%), and Cpn0308 by 82 (44.8%), 16 (26.7%), and 0 (0%) samples from groups I, II, and III, respectively. The positive recognition rates with any of the three antigens were significantly higher in group I than those in groups II and III, suggesting that more individuals from group I were likely infected with C. pneumoniae. This conclusion was confirmed with a commercially available whole organism‐based ELISA kit (Savyon Diagnostics Ltd., Ashdod, Israel), which detected C. pneumoniae antibodies in 98 (64.1%), 26 (43.3%), and 4 (12.5%) samples from group I, II, and III, respectively. Comparing to the commercial kit, the recombinant antigen‐based detection assays displayed >97% of detection specificity and >87% of sensitivity, suggesting that these recombinant antigens can be considered alternative tools for aiding in serodiagnosis of C. pneumoniae infection. J. Clin. Lab. Anal. 24:55–61, 2010. © 2010 Wiley‐Liss, Inc.
Growth‐dependent phenotypic variation of molluscan shells: implications for allometric data interpretation
Authors: Urdy Séverine , Goudemand Nicolas , Bucher Hugo , Chirat Régis 
Published: January 2010

AbstractIn recent years, developmental plasticity has received increasing attention. Specifically, some studies highlighted a possible association between shell shape and growth rates in intertidal gastropods. We use a growth vector model to study how hypothetical growth processes could underlie developmental plasticity in molluscs. It illustrates that variation in instantaneous shell growth rate can induce variability in allometric curves. Consequently, morphological variation is time‐dependent. Basing our model parameters on a study documenting the results of transplants experiments of three gastropods ecomorphs, we reproduce the main aspects of the variation in size, shape, and growth rates among populations when bred in their own habitat or transplanted to another ecotype habitat. In agreement with empirical results, our simulation shows that a flatter growth profile corresponds to conditions of rapid growth. The model also allows the comparison of allometric slopes using different subdata sets that correspond to static and ontogenetic allometry. Our model highlights that depending on subdata sets, the “main effects” could be attributed to source population or environment. In addition, convergence or divergence of allometric slopes is observed depending on the subdata sets. Although there is evidence that shell shape in gastropods is to some extent growth rate dependent, gaining a general overview of the issue is challenging, in particular because of the scarcity of studies referring to allometry. We argue that the dynamics of development at the “phenotypic level” constitute a non‐reducible level of investigation if one seeks to relate the observed amount of phenotypic variation to variability in the underlying factors. J. Exp. Zool. (Mol. Dev. Ecol.) 314B:303–326, 2010. © 2010 Wiley‐Liss, Inc.
Development and evaluation of a rapid immunochromatographic test for mumps‐specific IgM in oral fluid specimens and use as a matrix for preserving viral nucleic acid for RT‐PCR
Authors: Warrener Lenesha , Slibinskas Rimas , Brown David , Sasnauskas Kestutis , Samuel Dhanraj 
Published: January 2010

AbstractThe use of oral fluid specimens for diagnosis of mumps by detection of virus‐specific IgM or nucleic acid is now well established. The utility of oral fluids would be improved further if testing could be performed closer to the patient. A near patient test (NPT) for the detection of mumps‐specific IgM in oral fluid specimens was developed and evaluated using 196 oral fluid specimens from suspected cases of mumps and measles. Compared to enzyme immunoassay (EIA), the sensitivity, specificity, positive and negative predictive value of the mumps IgM NPT were 79.5%, 100%, 100%, and 72.6%, respectively. On oral fluid specimens with a test to negative control optical density ratio (T/N) ≥10 in the EIA, sensitivity of the NPT increased to 95.2%. To determine whether viral nucleic acid from oral fluid was preserved on the NPT strips used for IgM detection, reverse transcription‐polymerase chain reaction (RT‐PCR) was performed on 58 oral fluids before and after testing on NPT strips. In RT‐PCR‐positive oral fluids, mumps virus was detected in 19/21 (90.5%) specimens extracted from NPT strips. Mumps virus RNA was not detected in 37/37 (100%) NPT strips from mumps RT‐PCR‐negative oral fluid specimens. Mumps IgM NPT is rapid and simple to perform, with an acceptable level of performance for confirmation of a clinical diagnosis outside of the laboratory. The NPT strip is also a suitable matrix for preserving nucleic acid, enabling virus‐specific RT‐PCR to be performed. J. Med. Virol. 82:485–493, 2010. © 2010 Wiley‐Liss, Inc.
Detection and molecular characterization of human rotaviruses isolated in Italy and Albania
Authors: AnnaRita Petrinca , Grassi Tiziana , Donia Domenica , De Donno Antonella , Idolo Adele , Alfio Cristaldi , Alessandri Claudia , Alberto Spanò , Divizia Maurizio 
Published: January 2010

AbstractRotaviruses are one of the most important causes of gastroenteritis in children under 5 years old. Analysis of G and P rotavirus genotypes in circulation is crucial in evaluating the appropriacy of mass vaccination of children worldwide. Overall, 592 stool samples were collected in Tirana (Albania), the Salento peninsula (South Italy), and three different hospitals in Rome (Central Italy). Of the total samples, 31.3% were rotavirus positive in Albania, 78.3% in the Salento, and 40.3% in Rome. The samples collected in Tirana and Rome were G–P typed, whereas the samples collected in the Salento were only G typed. Overall, in Italy the most frequent combinations were G4 P[8] (54.5%), G1 P[8] (27.3%), and G2 P[4] (18.2%); in Albania they were G9 P[8] (72.1%), G4 P[8] (8.8%), G1 P[8] (5.9%), and G2 P[4] (2.9%). The prevalence in Albania of atypical combinations was 7.4% for G4 P[4] and 2.9% for G9 P[4]. Phylogenetic analysis was also performed to assess the genetic relatedness of the strains. J. Med. Virol. 82:510–518, 2010. © 2010 Wiley‐Liss, Inc.
Characterization of JC human polyomavirus infection in a Portuguese population
Authors: Matos Ana , Duque Vitor , Beato Sílvia , da Silva João Poiares , Major Eugene , Meliço‐Silvestre António 
Published: January 2010

AbstractJC virus (JCV) is ubiquitous in the human population, infecting children asymptomatically. After primary infection, JCV persists in the host throughout life and is often excreted in the urine. Two hundred thirty‐four urine samples and 78 serum samples, collected from 171 healthy individuals and 63 patients infected with HIV, were used to characterize JCV infection in a Portuguese population. Using PCR, JCV DNA was detected in 38% of the urine samples. A significant difference in the excretion rate was observed between patients infected with HIV (51%) and healthy individuals (33%). The frequency of JCV viruria increased with age in healthy individuals, but not in patients infected with HIV. JCV urinary load was determined by real‐time quantitative PCR and was independent of gender, age, HIV infection, and CD4+ cell count. Overall, the JCV genotype detected most commonly was 1B, followed by genotypes 2B and 4. The detection and quantitation of JCV‐specific antibodies were performed in serum samples by an established enzyme immunoassay (EIA). Antibodies to JCV were observed in 91% of the patients tested, irrespective of HIV infection. A positive correlation between JCV urinary load and antibody titers was demonstrated. The present study provides the first characterization of seroprevalence and urinary excretion of JCV in a Portuguese population and revealed similar results to those observed in other European countries. A comparison between healthy individuals and patients infected with HIV, despite identical values of seroprevalence, showed some differences in the pattern of urinary excretion. J. Med. Virol. 82:494–504, 2010. © 2010 Wiley‐Liss, Inc.
Molecular evolutionary analysis and mutational pattern of full‐length genomes of hepatitis B virus isolated from Belgian patients with different clinical manifestations
Authors: Pourkarim Mahmoud Reza , Amini‐Bavil‐Olyaee Samad , Verbeeck Jannick , Lemey Philippe , Zeller Mark , Rahman Mustafizur , Maes Piet , Nevens Frederik , Van Ranst Marc 
Published: January 2010

AbstractMolecular evolutionary patterns of 62 HBV full‐length genomes obtained from Belgian patients were characterized. Phylogenetic analysis revealed diverse HBV subgenotypes including A2 and A6 (46.8%), D1–D4 (38.8%), E (9.7%), C1 (1.6%), and B2 (1.6%). The study population consisted of patients with different ethnic origin (Caucasian, Turkish, Asian, Arab, and African). One HBV D/C recombinant isolate was identified, which encoded subtype adw2. An HBV subgenotype D4 with an aberrant subtype ayw4 was detected. Although none of the genotypes was associated with a specific disease outcome, several nucleotide substitutions, deletions and insertions were observed within the HBV preS1/S and X genes, particularly among patients with active chronic hepatitis B infection and patients with cirrhosis. Within the immunological domain of the HBsAg gene, the most frequent substitutions were sT125M and sT118A. High rates of precore and basal core promoter mutations were detected in patients infected with genotype D of HBV. Almost half of the patients who received lamivudine therapy for at least 1 year had HBV variants associated with lamivudine drug resistance. In conclusion, the most common HBV genotypes in West Europe (A and D) also prevail in Belgium. The highest degree of genetic diversity was detected in HBV genotype D. In addition, this study reveals the circulation of exotic HBV genotypes B, C, and E in Belgium. J. Med. Virol. 82:379–389, 2010. © 2010 Wiley‐Liss, Inc.
Adsorption of oligopeptides on silver nanoparticles: surface‐enhanced Raman scattering and theoretical studies
Authors: Garrido C. , Aliaga A. E. , Gomez‐Jeria J. S. , Clavijo R. E. , Campos‐Vallette M. M. , Sanchez‐Cortes S. 
Published: January 2010

AbstractThe MRKDV peptide structurally associated with an immunomodulatory protein, as well as model peptides ADEDRDA and LGRGISL with common amino acid residues were studied using surface enhanced Raman scattering (SERS) supported by quantum chemical computations. Peptides display different net charges and hydrophobic characteristics, which are related to particular structural aspects of the peptide–metal interaction. Samples were photostable when probed with laser lines at 514, 633 and 785 nm. SERS samples were prepared by coating the solid peptides with metal colloids on a quartz slice. This innovation makes possible to obtain high spectral batch to batch reproducibility. MRKDV SERS spectrum is dominated by signals coming from the guanidinium moiety of the arginine amino acid (R); guanidinium is the intrinsic probe which drives the orientation of the peptide on the metal surface. LGRGISL interacts with the metal surface through the guanidinium group and other amino acid residues; a single structural conformation of the peptide on the surface is proposed. ADEDRDA interacts with the metal surface through various amino acid residues, also including the guanidinium moiety; at least two different structural conformations seem to coexist on the surface. Theoretical calculations performed by using extended Hückel theory and 6‐31G* methods for a model of arginine interacting with a silver cluster support the observed experimental result. Similar calculations involving the MRKDV peptide are also reported. Copyright © 2010 John Wiley & Sons, Ltd.
Single‐beam coherent anti‐Stokes Raman scattering (CARS) spectroscopy of gas‐phase CO2 via phase and polarization shaping of a broadband continuum
Authors: Roy Sukesh , Wrzesinski Paul J. , Pestov Dmitry , Dantus Marcos , Gord James R. 
Published: January 2010

AbstractCoherent anti‐Stokes Raman scattering (CARS) spectroscopy of gas‐phase CO2 is demonstrated using a single femtosecond (fs) laser beam. A shaped ultrashort laser pulse with a transform‐limited temporal width of ∼7 fs and spectral bandwidth of ∼225 nm (∼3500 cm−1) is employed for simultaneous excitation of the CO2 Fermi dyads at ∼1285 and ∼1388 cm−1. CARS signal intensities for the two Raman transitions and their ratio as a function of pressure are presented. The signal‐to‐noise ratio of the single beam–generated CO2 CARS signal is sufficient to perform concentration measurements at a rate of 1 kHz. The implications of these experiments for measuring CO2 concentrations and rapid pressure fluctuations in hypersonic and detonation‐based chemically reacting flows are also discussed. Copyright © 2010 John Wiley & Sons, Ltd.
Cesium doped and undoped ZnO nanocrystalline thin films: a comparative study of structural and micro‐Raman investigation of optical phonons
Authors: Thangavel R. , Singh Moirangthem Rakesh , Lee Wei‐Shan , Chang Yia‐Chung , Wei Pei‐Kuen , Kumar J. 
Published: January 2010

AbstractUndoped and cesium‐doped zinc oxide (ZnO) thin films have been deposited on sapphire substrate (0001) using the sol–gel method. Films were preheated at 300 °C for 10 min and annealed at 600 and 800 °C for 1 h. The grown thin films were confirmed to be of wurtzite structure using X‐ray diffraction. Surface morphology of the films was analyzed using scanning electron microscopy. The photoluminescence (PL) spectra of ZnO showed a strong ultraviolet (UV) emission band located at 3.263 eV and a very weak visible emission associated with deep‐level defects. Cesium incorporation induced a blue shift of the optical band gap and quenching of the near‐band‐edge PL for nanocrystalline thin film at room temperatures because of the band‐filling effect of free carriers. A shift of about 10–15 cm−1 is observed for the first‐order longitudinal‐optical (LO) phonon Raman peak of the nanocrystals when compared to the LO phonon peak of bulk ZnO. The UV resonant Raman excitation at RT shows multiphonon LO modes up to fifth order. Copyright © 2010 John Wiley & Sons, Ltd.
On‐line flow‐through extraction–preconcentration‐large volume injection‐RP LC for trace determination of pyrethroids in Slovak soil
Authors: Chalányová Mária , Hutta Milan , Pagáč Martin 
Published: January 2010

AbstractA rapid micro‐analytical multiresidue method was developed for analysis of pyrethroids (kadethrin K, cypermethrin C and permethrin P) in soil micro‐sample (200 mg). It uses on‐line flow‐through extraction of soil micro‐samples (packed into a short glass column) with a methanol‐aqueous citric acid buffer mixture, successive on‐line SPE preconcentration of analytes from the extract and on‐line RP‐HPLC analysis with UV photometric detection. The separation of pyrethroids is performed on a Purospher RP‐18e column with methanol/water as mobile phase. Effects of sorbent placed at the bottom of a short column holding the soil sample and different kinds of on‐line SPE columns were tested. Besides, the influence of volume of the effluent on the pyrethroids recovery was also studied. Calibration curves were linear over the range assayed from 0.01 to 0.2 μg/mL with correlation coefficients of linear regression (least‐squares method) in the range 0.998–0.999. Recovery studies were carried out at 0.25–1.00 μg/g dry soil fortification level and obtained recoveries were for K 81–84%, C 56–59% and for P 58–63%. Achieved LOD (confidence band) of studied pyrethroids were for large‐volume injection (1 mL) 4.5 ng K, 3.7 ng C, 3.6 ng P or 27 ng/g K, 32 ng/g C and 29 ng/g P in dry soil “solid sampling HPLC”.
Rapid analysis of alkylphosphonate drugs by capillary zone electrophoresis using indirect ultraviolet detection
Authors: Prutthiwanasan Brompoj , Suntornsuk Leena 
Published: January 2010

AbstractA rapid capillary electrophoretic method for the analysis of three alkylphosphonate drugs (i.e. fosfomycin disodium (FOS), clodronate disodium (CLO) and alendronate sodium (ALN)) was developed by using multiple probe BGE and indirect UV detection. BGE containing 30 mM benzoic acid, 5 mM salicylic acid and 0.5 mM CTAB (pH 3.8), temperature of 30°C, applied voltage of −30 kV and detection at 220 nm provided baseline separation of all analytes (resolution (R)>2.2) in 3.2 min. EOF reversal by addition of CTAB and negative voltage polarity leading to the co‐EOF flow and short analysis time. Two probe BGE greatly improved peak symmetry. The method showed good linearity (r2>0.999 in ranges of 20–1000 μg/mL for FOS, 100–1000 μg/mL for CLO and 100–750 μg/mL for ALN) repeatablitiy (RSD<2.15%), recovery (99.3–101.1%) and sensitivity (LOD<50 μg/mL). Freshly prepared BGE and sample solutions are essential for the method precision and accuracy. This new method can be utilized for routine analysis of FOS, CLO and ALN in dosage forms because of its efficiency, reliability, speed and simplicity.
Suitability of ionic liquids as mobile‐phase additives in HPLC with fluorescence and UV detection for the determination of heterocyclic aromatic amines
Authors: Martín‐Calero Aurora , Tejral Gracian , Ayala Juan H. , González Venerando , Afonso Ana M. 
Published: January 2010

AbstractThe effects of several ionic liquids (ILs) as mobile‐phase additives in HPLC with fluorescence and UV–Vis detection for the determination of six heterocyclic aromatic amines were evaluated using two different C18 stationary phases with moderate silanol activity. The studied ILs were 1‐butyl‐3‐methylimidazolium tetrafluoroborate, 1‐hexyl‐3‐methylimidazolium tetrafluoroborate and 1‐methyl‐3‐octylimidazolium tetrafluoroborate. The optical behaviour of heterocyclic aromatic amines in presence of ILs was studied and the silanol‐suppressing potency of ILs was evaluated for the two stationary phases studied. Several chromatographic parameters were evaluated in the presence or absence of ILs, or using triethylamine, the most common mobile‐phase additive. The best results were achieved using 1 mM 1‐butyl‐3‐methylimidazolium tetrafluoroborate as mobile‐phase additive and NovaPak® column. In these conditions and with 18% of ACN in the mobile phase, analytical performance of the chromatographic methods using fluorescence and UV–Vis were evaluated, obtaining good precision in all cases (RSD lower than 6.6%) and low LOD (0.001–0.147 μg/mL with UV–Vis and 0.001–0.006 ng/mL with fluorescence detection).
An epistaxis severity score for hereditary hemorrhagic telangiectasia
Authors: Hoag Jeffrey B. , Terry Peter , Mitchell Sally , Reh Douglas , Merlo Christian A. 
Published: January 2010

AbstractObjectives/Hypothesis:Hereditary hemorrhagic telangiectasia (HHT)‐related epistaxis leads to alterations in social functioning and quality of life. Although more than 95% experience epistaxis, there is considerable variability of severity. Because no standardized method exists to measure epistaxis severity, the purpose of this study was to determine factors associated with patient‐reported severity to develop a severity score.Study Design:Prospective, survey‐based study.Methods:HHT care providers and a focus group of patients were interviewed to determine epistaxis‐associated factors. From this, an electronic survey was developed and administered to patients with HHT. Descriptive analyses were performed with calculations of means and medians for continuous and proportions for categorical variables. Multiple ordinal logistic and linear regression models were developed to determine risk factors for epistaxis severity.Results:Nine hundred respondents from 21 countries were included. Eight hundred fifty‐five (95%) subjects reported epistaxis. The mean (standard deviation) age was 52.1 (13.9) years, and 61.4% were female. Independently associated risk factors for self‐reported epistaxis severity included epistaxis frequency (odds ratio [OR] 1.57), duration (OR 2.17), intensity (OR 2.45), need for transfusion (OR 2.74), anemia (OR 1.44), and aggressiveness of treatment required (OR 1.53, P < .001 for all).Conclusions:Risk factors for increasing epistaxis severity in patients with HHT include frequency, duration, and intensity of episodes; invasiveness of prior therapy required to stop epistaxis; anemia; and the need for blood transfusion. From these factors, an epistaxis severity score will be presented. Laryngoscope, 2010
Co‐continuous Nanostructured Blend by Reactive Blending: Incorporation of High Molecular Weight Polymers
Authors: Gani Léa , Tencé‐Girault Sylvie , Milléquant Michèle , Bizet Stéphane , Leibler Ludwik 
Published: January 2010

AbstractOne of the most challenging issues remaining in the design and the synthesis of robust nanostructured polymers blends is incorporating long crystallizable chains while preserving the co‐continuity of the phases. Here, we demonstrate that by reactive blending of functionalized polyolefin and polyamides such co‐continuous structures can be obtained provided that a bimodal mixture of short and long polyamides is used. The short chains react more readily to form graft copolymers that facilitate both grafting of long chains and formation of nanostructures. We discuss different mixing strategies, characterize, and compare reacted products and resulting blend structures.magnified image
Curcumin inhibits tumor growth and angiogenesis in glioblastoma xenografts
Authors: Perry Marie‐Claude , Demeule Michel , Régina Anthony , Moumdjian Robert , Béliveau Richard 
Published: January 2010

AbstractAmong the natural products shown to possess chemopreventive and anticancer properties, curcumin is one of the most potent. In the current study, we investigated the effects of this natural product on the growth of human glioma U‐87 cells xenografted into athymic mice. We show here that curcumin administration exerted significant anti‐tumor effects on subcutaneous and intracerebral gliomas as demonstrated by the slower tumor growth rate and the increase of animal survival time. While investigating the mechanism of its actionin vivo, we observed that curcumin decreased the gelatinolytic activities of matrix metalloproteinase‐9. Furthermore, treatment with curcumin inhibited glioma‐induced angiogenesis as indicated by the decrease of endothelial cell marker from newly formed vessels and by the diminution of the concentration of hemoglobin in curcumin‐treaded tumors. We also demonstrate, using anin vitromodel of blood−brain barrier, that curcumin can cross the blood−brain barrier to a high level. These are the first results showing that curcumin suppresses tumor growth of gliomas in xenograft models. The mechanisms of the anti‐tumor effects of curcumin were related, at least partly, to the inhibition of glioma‐induced angiogenesis.