bluuebunny/crossref_metadata_2025_split huggingface dataset:
Artistic approach to the WFS system
Authors: Kang Ji Youn 
Published: June 2024

This paper, “Artistic Approach to the Wave Field Synthesis system,” explores the diverse characteristics of the WFS system as a musical instrument and examines artistic approaches to spatializing sounds in electroacoustic music composition. It delves into the unique attributes of the system, providing detailed examples of compositions and discussing potential challenges and limitations. The paper raises questions about how such a spatial audio rendering system can impact our listening experience, particularly in relation to fixed media compositions. Additionally, the possibilities of using WFS in live electronic music are also explored.
Association between Dietary Habits and Nutritional Status among Adolescent Girls
Authors: Al-Mosawy Huda Thiar M. , Faris Selman Hussain 
Published: June 2024

Background: Adolescents with good eating habits are more likely than those with bad eating habits to learn normally and do better academically. Dietary habits refer to the long-term dietary patterns that an individual forms and maintains in his daily life.
Objectives: The study aims to assess influence of dietary habits of adolescent girls on nutritional status and identify if their association between pattern of dietary habits and nutritional status among adolescent girls.
Method: A cross-sectional study was conducted with 400 students from middle and secondary schools in Karbala City, Iraq. Data were collected from December 2o, 2023 to February 4, 2024, Data collection was performed using self-report instrument and it is organized into three sections, include, dietary habits, nutritional status and sociodemographic data. Data were analyzed utilizing descriptive and inferential statistics.
Result: That 61% of female adolescents have normal weight and 20.2% of female adolescents have underweight but 12% of them have overweight. Exhibits that female adolescent having moderate dietary habits as reported among 73% of them (M±SD= 11.96 ± 1.81.
Conclusion: The eating habits of adolescent’s girls do not affect the nutritional status, and most of them have healthy eating habits, while about one fifth of them have unhealthy eating habits, and more than a third of them had an abnormal body mass. Which indicates the importance of involving them in healthy awareness programs about healthy nutrition and ways to maintain a normal level of body mass.
A Reproducible Manuscript Workflow With a Quarto Template
Authors: Erickson Richard A. , Archer Althea A. , Fienen Michael N. 
Published: June 2024

Abstract
Scientists and resource managers increasingly use Markdown-based tools to create reproducible reports and manuscripts. These workflows allow people to use standardized methods that are more reproducible, efficient, and transparent than other standard office tools. We present a Quarto template and demonstrate how this template may be used for the Journal of Fish and Wildlife Management. This template may also be readily adapted to other journals that use Microsoft Word-based workflows and for other product types such as annual reports. We also provide a high-level overview of Quarto and other Markdown-based workflows. Last, we provide examples of some features of the Quarto publishing system that may be helpful for authors when customizing Quarto templates for specific journal formatting requirements and other product types.
Das Manövrieren inmitten der zahlreichen Wendepunkte der STS
Authors: Paulos Julio , Riom Loïc 
Published: June 2024

Dieser Text ist eine spekulative Reflexion über die Abfassung eines internationalen Konferenzaufrufs – Call for Papers. Er spiegelt diese einzigartige Übung wider, sich zu einem bestimmten Thema an eine Gemeinschaft von Fachleuten zu wenden. Die Frage der materiellen Fragilität, der sich dieser Call for Papers widmet, wird in diesem Sinne nicht konzeptionell oder methodologisch, sondern aus einer forschungspraktischen Perspektive behandelt. In Form eines Briefwechsels wird berichtet, wie ein „Wendepunkt“ oder ein disziplinärer Moment die Art und Weise, wie Forschung betrieben und gedacht wird, initiiert, beeinflusst und lenkt.
Neutrophil Heterogeneity Is Modified during Acute Lung Inflammation in Apoa1−/− Mice
Authors: Yao Xianglan , Redekar Neelam R , Keeran Karen J , Qu Xuan , Jeffries Kenneth R , Soria-Florido M T , Saxena Ankit , Dagur Pradeep K , Lin Wan-Chi , McCoy J Philip , Levine Stewart J 
Published: June 2024

Abstract
Neutrophils play important roles in inflammatory airway diseases. In this study, we assessed whether apolipoprotein A-I modifies neutrophil heterogeneity as part of the mechanism by which it attenuates acute airway inflammation. Neutrophilic airway inflammation was induced by daily intranasal administration of LPS plus house dust mite (LPS+HDM) to Apoa1−/− and Apoa1+/+ mice for 3 d. Single-cell RNA sequencing was performed on cells recovered from bronchoalveolar lavage fluid on day 4. Unsupervised profiling identified 10 clusters of neutrophils in bronchoalveolar lavage fluid from Apoa1−/− and Apoa1+/+ mice. LPS+HDM-challenged Apoa1−/− mice had an increased proportion of the Neu4 neutrophil cluster that expressed S100a8, S100a9, and Mmp8 and had high maturation, aggregation, and TLR4 binding scores. There was also an increase in the Neu6 cluster of immature neutrophils, whereas neutrophil clusters expressing IFN-stimulated genes were decreased. An unsupervised trajectory analysis showed that Neu4 represented a distinct lineage in Apoa1−/− mice. LPS+HDM-challenged Apoa1−/− mice also had an increased proportion of recruited airspace macrophages, which was associated with a reciprocal reduction in resident airspace macrophages. Increased expression of a common set of proinflammatory genes, S100a8, S100a9, and Lcn2, was present in all neutrophils and airspace macrophages from LPS+HDM-challenged Apoa1−/− mice. These findings show that Apoa1−/− mice have increases in specific neutrophil and macrophage clusters in the lung during acute inflammation mediated by LPS+HDM, as well as enhanced expression of a common set of proinflammatory genes. This suggests that modifications in neutrophil and macrophage heterogeneity contribute to the mechanism by which apolipoprotein A-I attenuates acute airway inflammation.
Identification of Regulatory RNA-Binding Proteins Associated with Immune Infiltration in Laryngeal Squamous Cell Carcinoma
Authors: Xu Xin , Yang Xi , Tang Jv , Wu Xiaoguang , He Xiaoguang 
Published: June 2024

Abstract
We analyzed bulk RNA sequencing and single-cell RNA sequencing (scRNA-seq) data to identify alternative splicing (AS) events and regulatory RNA-binding proteins (RBPs) associated with immune infiltration in human laryngeal squamous cell carcinoma (LSCC). Whole-transcriptome sequencing data of 20 human laryngeal cancer and paracancerous tissues were downloaded from the Gene Expression Omnibus public database, using newly published splicing-site usage variation analysis software to obtain highly conserved regulated AS (RAS) events, and scientific reverse convolution algorithm analysis was used to identify significantly different immune cells and perform a correlation analysis between the two. The software package edgeR was used to identify differentially expressed RBPs and the immune infiltration-related LSCC-RAS they may regulate. Finally, we present the expression profiles and survival curves of 117 human laryngeal cancer samples from The Cancer Genome Atlas dataset for the identified RBPs and LSCC-RAS. We also downloaded the gene set enrichment 150321 scRNA-seq data for two human LSCC tissue samples. The RBP expression pattern and the expression of prophase RBP genes were analyzed in different LSCC cell populations. RNA-binding motif protein 47 (RBM47) and filamin A, as well as the RBP-RAS events that were screened in both the fibulin 2 and fibronectin 1 genes, were all significantly associated with the prognosis, and the RBM47 gene was upregulated in myeloid cells. Because the prognosis was significantly associated with two RBP regulators and two LSCC-RAS events, they may be critical regulators of immune cell survival during laryngeal cancer progression, and RBM47 may regulate macrophage-associated AS and affect immunity.
Reappraising the Role of T Cell–Derived IFN-γ in Restriction of Mycobacterium tuberculosis in the Murine Lung
Authors: Maciag Karolina , Plumlee Courtney R , Cohen Sara B , Gern Benjamin H , Urdahl Kevin B 
Published: June 2024

Abstract
T cells producing IFN-γ have long been considered a stalwart for immune protection against Mycobacterium tuberculosis (Mtb), but their relative importance to pulmonary immunity has been challenged by murine studies that achieved protection by adoptively transferred Mtb-specific IFN-γ−/− T cells. Using IFN-γ−/− T cell chimeric mice and adoptive transfer of IFN-γ−/− T cells into TCRβ−/−δ−/− mice, we demonstrate that control of lung Mtb burden is in fact dependent on T cell–derived IFN-γ, and, furthermore, mice selectively deficient in T cell–derived IFN-γ develop exacerbated disease compared with T cell–deficient control animals, despite equivalent lung bacterial burdens. Deficiency in T cell–derived IFN-γ skews infected and bystander monocyte-derived macrophages to an alternative M2 phenotype and promotes neutrophil and eosinophil influx. Our studies support an important role for T cell–derived IFN-γ in pulmonary immunity against tuberculosis.
Expert opinion on the management of patients with osteoporosis with anabolic drugs in Italy
Authors: Rossini M. , Cantatore F.P. , Del Puente A. , Frediani B. , Gatti D. , Giannini S. , Varenna M. , Viapiana O. , Sebastiani G.D. 
Published: June 2024

Objective. Fragility fractures (FF) resulting from osteoporosis pose a significant public health challenge in Italy, with considerable socio-health and economic implications. Despite the availability of safe and effective drugs, osteoporosis remains underdiagnosed and undertreated, leaving over 2 million high-risk Italian women without treatment. This paper aims to identify and propose key improvements in the management of osteoporosis, focusing particularly on the critical issues related to the use of anabolic drugs in secondary prevention, according to the current Italian Medicines Agency (AIFA) Note 79.
Methods. The Expert Panel, composed of nine recognized Italian experts in rheumatology, analyzed current practices, prescribing criteria, and the most recent literature. Three main reasons for revising the indications on pharmacological treatment of osteoporosis were identified: inadequate treatment of osteoporosis, new evidence regarding frontline placement of anabolics in high-risk conditions, and emerging sequential or combined strategies.
Results. The proposed improvements include the adoption of the Derived Fracture Risk Assessment algorithm for accurate fracture risk assessment, revision of AIFA Note 79 to reflect current evidence, improved prescribing appropriateness, broader access to anabolic agents, and the provision of sequential therapies with antiresorptives for teriparatide. These changes aim to enhance patient outcomes, streamline healthcare processes, and address the high percentage of undertreated individuals.
Conclusions. This expert opinion emphasizes the importance of the appropriate use of anabolic drugs to reduce FF and associated costs while ensuring the sustainability of the National Health Service. The proposed recommendations are in line with the latest scientific evidence, providing a comprehensive strategy to optimize the management of osteoporosis in Italy.

On behalf of the Study Group on Osteoporosis and Skeletal Metabolic Diseases of the Italian Society of Rheumatology.
Antioxidative effects of α-tocopherol on stored human red blood cell units
Authors: Hajizamani Saeideh , Atarodi Kamran , Deyhim Mohammad Reza , Kermani Fahimeh Ranjbar , Hosseini Kamran Mousavi 
Published: June 2024

Abstract:

BACKGROUND:
Red blood cell (RBC) units undergo metabolic, structural, and biochemical changes known as “storage lesions” that can reduce the survival and quality of RBCs. The use of antioxidants such as α-tocopherol may help to improve the quality of RBC units by reducing oxidative stress. The aim of this study was to determine the antioxidant effect of α-tocopherol in RBC units containing citrate-phosphate-dextrose solution with adenine (CPDA1) stored at 1°C–6°C for 35 days.

MATERIALS AND METHODS:
Four RBC units containing CPDA1 were divided into four equal satellite bags. Three bags were supplemented with 0.125, 0.625, and 3.125 mM concentrations of α-tocopherol as test groups. One bag was supplemented with ethanol (0.5%) as a control group. They were stored at 1°C–6°C for 35 days. Malondialdehyde (MDA) concentration, total antioxidant capacity (TAC), and hemolysis index (HI) were measured on days 0, 7, 14, 21, 28, and 35.

RESULTS:
In all groups, MDA concentration and HI increased and TAC decreased (P < 0.05). MDA concentration and HI in the 3.125 mM of the α-tocopherol group had a lower increase compared to the other test and control groups. Supplementation of RBC units with α-tocopherol resulted in a significant increase of TAC in all three groups compared to the control group (P < 0.05) and had a lower reduction during storage.

CONCLUSION:
Supplementation of RBC units with α-tocopherol improves the quality of RBC units by decreasing lipid peroxidation and hemolysis and by increasing TAC. Among the mentioned concentrations, 3.125 mM of α-tocopherol had a significantly more antioxidant effect.
Rh and Kell phenotyping in voluntary blood donors: A study from a tertiary care blood center of western India
Authors: Shah Sangita D. , Bhatnagar Nidhi M. , Shah Mamta C. , Thakkar Garima H. , Ahuja Ujjwal , Patel Anu , Gajera Drashti , Kalavadiya Priya G. 
Published: June 2024

Abstract:

INTRODUCTION:
In human beings, there are 45 blood group systems and 360 antigens currently recognized by ISBT (July 2023). The Rh blood group system has 56 antigens, out of them 5 antigens D, C, c, E, and e are clinically significant antigens. The Kell blood group system has 25 highly immunogenic antigens. Cases have been reported where IgG-type of antibodies against Rh and Kell antigens are found which are responsible for transfusion reactions and hemolytic disease of newborn.

AIMS AND OBJECTIVES:
To study the prevalence of Rh-Kell phenotype in voluntary blood donors, To provide Rh and Kell antigen-matched blood products to patients to prevent alloimmunization, To make a donor directory of Rh and Kell phenotyped donors for further use.

MATEIALS AND METHODS:
The antigen typing for Rh antigens (D, C, c, E, and e) and Kell (K) was performed on the collected ethylenediaminetetraacetic acid samples from 1014 voluntary donors. The test was performed by Erythrocyte Magnetic Technique using a microplate (DuoLys) in a fully automated immunohematology system (Diagast Qwalys Evo 3 instrument).

RESULTS:
From 1014 phenotyped donors, the most common antigen frequency was of “e” (98.6%) followed by “D”(96.2%),“C”(89.4%), “c”(54.8%), and “E”(18.6%). The frequency of the “K” antigen was (1.38%). The most common Rh phenotype from the study population was R1R1(CDe/CDe) (45%) and the rarest was r’r’ (Ce/Ce) (0.1%).

CONCLUSION:
Knowledge of the phenotype frequency in the local population is helpful in making a donor directory, In situations where clinically significant alloantibodies are found in patient’s serum, antigen-negative blood unit can be arranged using a donor directory.
Seroprevalence of COVID-19 among voluntary blood donors
Authors: Pothipillai Arumugam , Hamsavardhini Swathandran , Duraisamy Deepa , Thiyagarajan Lincy , Kaliyaperumal Chandrasekaran , Rao Jyotsnaa Grace Velure Mohan 
Published: June 2024

Abstract:

CONTEXT:
COVID-19 usually presents with mild symptoms. No cases of transfusion – transmission of COVID-19 had been reported. Assessing the prevalence of viral infections among blood donors is essential to frame blood safety strategies.

AIM:
The main aim of this study is to assess the seroprevalence of SARS-CoV-2 antibodies among healthy and asymptomatic voluntary blood donors by enzyme-linked immunosorbent assay (ELISA).

SETTING AND DESIGN:
This cross-sectional study was conducted among voluntary blood donors using a consecutive sampling technique in the Department of Transfusion Medicine, the Tamil Nadu Dr. M.G.R. Medical University, Guindy, Chennai, for 18 months.

METHODS:
Adhering to COVID-19 pandemic guidelines and donor eligibility criteria, blood samples collected from 500 asymptomatic unvaccinated voluntary blood donors were tested for SARS-CoV-2 (IgG + IgM + IgA and IgG) antibodies by ELISA. Adding IgA to a conventional IgM and IgG serological test improves sensitivity.

STATISTICAL ANALYSIS USED:
The collected donor data were analyzed with IBM SPSS Statistics software. Pearson’s Chi-square test and Fisher’s exact test were used. P = 0.05 was considered statistically significant.

RESULTS:
IgG seropositivity among the donors was 58.8%, and IgM + IgA seropositivity was 29.6%. There was no statistically significant difference in the COVID-19 IgG/IgM + IgA seropositivity status with age, gender, blood group, occupation, or socioeconomic status. The IgG and IgM/IgA/IgG ELISA kits showed a difference of 13 cases which could be attributed to the higher sensitivity of IgG alone ELISA kit. This increased the seroprevalence by 3%.

CONCLUSION:
The majority of donors were either IgG or IgM and IgA positive, despite remaining asymptomatic. The seropositivity rate coincided with the COVID-19 surge among population.
Early Tangential Excision and Split-Thickness Skin Graft Reduced Hospitalized Length Stays for Burn Injuries
Authors: Kusuma Angga Putra , Saputro Iswinarno Doso , Handriani Ira , Aung Zin Mar , Swaminathan Vijayendran 
Published: June 2024

ABSTRACT

Introduction:
Burns pose a physical, psychological, health system, and long-term length of stay problem. Surgical treatment of burns is an important intervention in their management. The optimal timing of early tangential excision and split-thickness skin graft varies. Early excision of burned tissue to remove dead tissue and inhibit the inflammatory process decreases the risk of infection. Early excision and grafting of burns is the standard of practice for most major burns.

Methods:
The research was a cross-sectional study with a descriptive design of secondary data. Burn patients were treated at Dr. Soetomo General Academic Hospital Surabaya, Indonesia, from 2018 to 2022. Inclusion criteria in this study were all patients who underwent early tangential excision and split-thickness skin graft. Achieving statistical analysis and data analysis using SPSS 25 on Windows.

Results:
A greater amount of time on total body surface area (TBSA) is linked to early tangential excision and split-thickness skin grafts. There are statistically significant variations in the burn area (TBSA 20.5%–30%, P = 0.036 (P = 0.05), TBSA 30.5%–40%, P < 0.001, and burn area (TBSA 40.5%–50%, P = 0.003). The current findings demonstrated that split-thickness skin grafts and early tangential excisions done on burns with TBSA 20% at partial thickness–full thickness depth had statistically significant outcomes, shortening the length of stay for burn patients.

Conclusions:
An analysis of the current data, burn patients’ duration of stay was reduced when burns with TBSA >20% with partial thickness–full thickness depth underwent early tangential excision and split–thickness skin transplant had statistically positive results.
Normal Weight Obesity Associated with Enhanced Echo Intensity, Insulin Resistance, and Decreased Muscle Strength in Young Adults
Authors: Aruna R , Sivarajan Armel Arputha , Revathy Gunaseelan , Vasanth CJ 
Published: June 2024

Abstract

Context:
Obesity refers to the accumulation of excess body fat. Even individuals with a normal body mass index (BMI) of <23 Kg/m2 but a high percentage of body fat have an increased risk for noncommunicable disease. This condition is referred to as normal weight obesity (NWO).

Aim:
To assess the association between muscle strength, ultrasound-derived muscle thickness, insulin resistance, and echo intensity in NWO and normal weight nonobese (NWNO) young adults.

Methods and Material:
Eighty young adults of a normal BMI of 18.5 to 22.9 Kg/m2 were recruited in this current study. Forty subjects with cutoff points of body fat percentage ≥ 20.6% for men and ≥ 33.4% for women were taken as NWO group and 40 were NWNO. Fasting glucose and insulin were used for calculating insulin resistance, sensitivity, and beta cell function. Ultrasonography (USG) was done to measure forearm radial and ulna muscle thickness. The echo intensity was measured from an ultrasound image as a mean pixel value using Adobe Photoshop. Subjects were tested for handgrip strength using hand dynamometry.

Results:
Hand grip strength was higher in NWNO group, and subcutaneous fat thickness and echo intensity were higher in the NWO group. Glucose, insulin, and insulin resistance were significantly higher in the NWO group. Linear regression showed a significant correlation between ulna muscle thickness, forearm circumference, echo intensity, and insulin resistance with handgrip strength (r = 0.835 P < 0.001).

Conclusions:
NWO adults have higher insulin resistance and echo intensity, indicating decreased muscle quality compared to NWNO individuals.
Soil-transmitted Helminth Infection and Perinatal Outcomes in Pregnant Women in Primary Care Settings in South India: A Cohort Study
Authors: Ulaganeethi Revathi , Dorairajan Gowri , Rajkumari Nonika , Jayaraman Suganya , Subramanian Sadhana , Saya Ganesh Kumar 
Published: June 2024

Background:
Soil-transmitted helminth infections (STHs) in pregnancy have been linked to maternal anemia, low birth weight, and adverse perinatal outcomes. STH includes Ascaris lumbricoides, hookworm, and Trichuris trichiura. However, conflicting evidence exists regarding these effects. Hence, we aimed to assess the association of STH infections with perinatal outcomes.

Methods and Materials:
A community-based cohort study was conducted among pregnant women in primary care settings in Puducherry, South India, during December 2019 to April 2021. Stool samples were collected for STH screening and confirmed using polymerase chain reaction. Information on perinatal outcomes and hemoglobin levels were extracted from case records. The association of pre-term birth, low birth weight, anemia, and miscarriages with STH infection was analyzed using the Chi-square test.

Results:
Among the 650 women enrolled, 58 (8.9%) had one of the STH infections. At the time of recruitment, 336 (52.0%) women had anemia. Twenty-four women (3.7%) had miscarriages, 14 (2.2%) had pre-term birth, and 65 (10.4%) had low-birth-weight (LBW) babies. All 14 pre-term babies had LBW, and out of 65 LBW, 40 were in mothers with anemia. Incidence of LBW, pre-term deliveries, and anemia were higher in women with STH than in those without STH (LBW: 12.3% vs 10.2%, P = 0.62; pre-term: 3.5% vs 2.1%, P = 0.5; and anemia: 54.4% vs 51.85, P = 0.7).

Conclusion:
Though the incidence of anemia, LBW, and pre-term delivery was higher in women with STH infections compared to those without STH, we did not find any significant association between the presence of STH infections and perinatal outcomes.