bluuebunny/crossref_metadata_2025_split huggingface dataset:
Uncontrolled hypertension is associated with an increased risk of end-stage renal disease in patients with type 2 diabetes who underwent postpercutaneous coronary intervention: A nationwide population-based study
Authors: Kim Hyunglae , Park Yong-Moon , Ko Seung-Hyun , Cho Yun-Jung , Kim Hyung Wook , Kim Yong Kyun , Han Kyung-Do , Yun Jae-Seung , Kim Kyuho , Moon Donggyu 
Published: May 2024

Abstract

We investigated the effect of differential blood pressure (BP) levels on future end-stage renal disease (ESRD) in patients with type 2 diabetes mellitus (T2DM) and established coronary artery disease following percutaneous coronary intervention (PCI). Using health check-up data from the Korean National Health Insurance Service (2015–2016), we analyzed 80,187 T2DM patients who underwent PCI. Patients were categorized by blood pressure (BP) levels measured post-PCI: systolic BP < 120 (reference), < 130, <140, < 160, and ≥160 mm Hg; diastolic BP < 80 (reference), < 90, <100, and ≥100 mm Hg. Incident ESRD, defined by disease codes and renal replacement therapy initiation, was the primary outcome. Multivariate Cox proportional hazard regression assessed adjusted hazard ratios (HRs) (95% CI) by BP group. Mean age was 67.7 years; 80.9% used antihypertensives. ESRD incidence was 1.70% (1,362 patients) over 4.7 years. After adjustment for confounding factors, the HR of ESRD patients significantly and sequentially increased in the higher BP groups. Similar trends were seen with pulse pressure (PP). Subgroup analysis showed stronger SBP-ESRD association in < 65-year-olds (interaction P = 0.0498). ESRD risk linearly rose with systolic BP and had a J-shaped association with diastolic BP, especially at baseline values of 70 and 68 mm Hg. Elevated BP, including PP, correlated with ESRD risk in a dose‒response manner among T2DM patients who underwent PCI. Strict BP control is crucial for preventing ESRD in this population.
Impact of phenylalanine and ultrasonic waves on the production and release of Taxol in hazelnut (Corylus avellana L.) cell culture
Authors: Jamali Maryam , Ghanbari Alireza , Estaji Asghar , Chamani Esmaeil , Naji Amir Mohammad , Maleki Hassan 
Published: May 2024

Abstract

Hazelnut is one of the most important nut plants recently suggested as a sustainable source of Taxol. Taxolone is one of the most commonly prescribed chemotherapy drugs for cancer and is widely used for various types of cancer. In the present study, the effect of the phenylalanine concentration (5, 7.5 and 12.5 mg. L− 1) and ultrasonic waves (for 30, 45, 60 and 90 s) were investigated for their effects on cell induction and growth and Taxol and other secondary metabolite production in hazelnut cell cultures. This study used a completely randomized experimental design. The results showed that enhancing the UT for up to 45 s led to an increase in both fresh matter (Fm) and dry matter (Dm) to a certain extent, after which a decreasing trend was observed. An examination of the effects of the treatments on the accumulation of secondary metabolites revealed that phenylalanine and UT had positive effects on these indices, with the highest TFC (1.95 mg. g− 1) was observed in samples grown at 12.5 mg.L1. Moreover, our results showed that UT had a positive impact on the production of Taxol in Hazelnut cell suspensions. Specifically, UT at 45 s had the greatest amount of Taxol (208.41 ppm) in the Hazelnut cell suspension.
Implementing Quality by Design (Qbd) Strategy for Optimization of lyophilized Solid Lipid Nanoparticles to Boost Oral Bioavailability of antimalarial drug
Authors: Bajwa Neha , Singh Preet Amol , Madan Jitender , Baldi Ashish 
Published: May 2024

Abstract

Background:
Arteether (ART) is artemisinin derivatives drug used for resistant malaria. It has oral bioavailability of almost 0.98 %.
AIM:
ART loaded solid lipid nanoparticles (SLN) were developed with enhanced bioavailability using Quality by Design approach.
Methodology:
ART loaded SLNs were prepared by solvent emulsification/evaporation method using Quality by Design approach. Surfactant concentration and acetone to ethanol volume ratio were selected as independent variable while particle size and entrapment efficiency was selected as responses using central composite design.
Result:
The produced SLN were lyophilized and the powdered SLNs was encapsulated in an enteric coated capsule shell. The particle diameters of all the formulations were between 109 and 250 nm, and the entrapment effectiveness was 93.7 %. The XRD spectrum revealed that the ART was in amorphous form. The ART-SLNs release pattern revealed that ART was released in a slow yet time-dependent manner, which seems beneficial to prevent it from acid degradation.
The permeability of ART containing formulations was investigated using the Franz diffusion cell technique. The concentration of ART employing ART-SLN to pure ART in the pig's intestine was nearly 7.1 fold enhanced.
The pharmacokinetics of ART-SLN administered orally to rabbit models was investigated. The bioavailability of ART-loaded SLN was increased to 27.64%.
Conclusion:
These findings suggest that formulation development by using quality-by-design in present study also provide a comprehensive solution to develop oral formulation of arteether with desired bioavailability at industrial scale.
Decoding In-Cell Respiratory Enzyme Dynamics by Label-Free In-situ Electrochemistry
Authors: Tokunou Yoshihide , Yamazaki Tomohiko , Okamoto Akihiro 
Published: May 2024

Abstract

Deciphering metabolic enzyme catalysis in living cells remains a formidable challenge due to the limitations of in vivo assays, which focus on enzymes isolated from respiration. This study introduces an innovative whole-cell electrochemical assay to reveal the Michaelis-Menten landscape of metabolic enzymes amid complex molecular interactions. We controlled the microbial current generation's rate-limiting step, extracting in vivo kinetic parameters (Km, Ki, and kcat) for the periplasmic nitrite and fumarate (FccA) reductases. Despite deleting CymA, a key electron donor, alternative electron transfer pathways sustained the FccA activity. This enabled direct observation of FccA-CymA interaction, uncovering the pivotal role of CymA in altering the post-binding dynamics of FccA, such as catalysis and product release. This finding challenges the long-held belief that the molecular crowding effect primarily drives discrepancies between in vivo and in vitro kinetics. This work offers significant leap in understanding cellular enzymatic processes and opens avenues for future biochemical research.
A probabilistic approach for the Levelized cost of energy of floating offshore wind farms
Authors: Amlashi Hadi , Baniotopoulos Charalampos 
Published: May 2024

Abstract

This paper aims to analyze the levelized cost of energy (LCOE) of floating offshore wind farms from a probabilistic point of view. Understanding and addressing the uncertainty associated with the main parameters that influence the wind energy generated by a wind farm during its lifetime is crucial for the economic evaluation of offshore wind energy in the broader energy landscape. The methodology for probabilistic assessment of LCOE is introduced, and the uncertainty in input parameters are discussed. In a base case study, an assumed Floating Offshore Wind Farm (FOWT) consisting of 250 5-MW wind turbines is considered. The use of bias and randomness in key random variables is discussed and studied in detail. Results indicate that LCOE estimates of 15 EURc/KWh for offshore wind turbines are achievable with reasonable confidence, while estimates of 5 EURc/KWh require careful consideration of uncertainty in the wind farm’s parameters. The feasibility analysis showed that financial parameters are more influenced by wind characteristics and efficient use of wind turbines than by the cost of the wind farm. This paper provides general guidance on how to carry out early-stage economic analysis of FOWFs.
Efficient Electrochemical Hydrogen Peroxide Production Enabled by Defect Engineering of Epoxy-Rich Carbon Materials
Authors: Liu Yijian , Gan Lang 
Published: May 2024

Abstract

Introducing oxygenated species into carbon-based catalysts is an efficient method to enhance the activity of electrochemical hydrogen peroxide (H2O2) production. Here, we report a low-temperature plasma strategy for preparing carbon materials rich in epoxy groups. This catalyst demonstrates effective electrocatalytic activity with a H2O2 selectivity over 90% and a Faraday efficiency of 94.9% after 12h of stable H2O2 production. The exceptional electrochemical activity is attributed to the epoxy group species, which are formed through the reaction between carbon defects and oxygen. This work offers an effective method for preparing carbon-based catalysts for H2O2 production and provides insights into the impact of oxygenated species on the activity for H2O2 generation.
In situ phenotypic and karyotypic co-detection of aneuploid TCs and TECs in cytological specimens with abnormal cervical screening results
Authors: Wang yanling , Lin Alexander Y , Wang Daisy Dandan , Lin Peter Ping , Zhou Xuexin , Yang Yongbin , Zhu Yaping 
Published: May 2024

Abstract

Background
Detection of chromosome aneuploidy is an important method for cervical cancer screening, however, the study of chromosome ploidy in primary cervical tumor cells is limited. A novel immunostaining integrated with fluorescence in situ hybridization (iFISH) strategy phenotypically and karyotypically co-detected the expression of tumor markers and chromosome aneuploidy to investigate the diagnostic values of aneuploid tumor cells (TCs) and tumor endothelial cells (TECs) in all-stage cervical lesion smear specimens.
Methods
A total of 196 patients were enrolled in this study. Immunofluorescence staining of p16 and Ki67 combined with FISH was applied to quantitatively co-detect and characterize aneuploid CD31− TCs and CD31+ TECs as well as their subtypes in cervical cytological specimens. The diagnostic values of aneuploid TCs and TECs for high-grade squamous intraepithelial lesions (HSIL+) were investigated by receiver operating characteristic curve analysis.
Results
The number of total aneuploid CD31− TCs and their p16/Ki67+ subtypes increased markedly with the severity of cervical lesions, although a similar was not observed on aneuploid CD31+ TECs. To identify HSIL+, the area under the curve (AUC) of tetraploid TCs was the largest (0.739), followed by multiploidy (≥ pentaploid) TCs (0.724) and triploid TCs (0.699). Regarding combined subtypes, the AUC of ≥ tetraploid TCs was 0.745, and their unique diagnosis values were clinically reflected in the vitally high specificity.
Conclusion
The number of CD31− TCs was associated with the severity of cervical lesions and aneuploid CD31− TCs exhibited a remarkable diagnostic specificity for HSIL+. Further studies are required to broaden their other potential clinical utility.
A retrospective study on risk factors for low vision in patients with traumatic hyphema
Authors: Hao Zhuang , Zhou Wenqian , Wang Jue , Zhang Jie 
Published: May 2024

Abstract

Background: This study examines the risk factors associated with low vision following traumatic hyphema to enhance prognosis evaluation.
Methods: A retrospective analysis was performed on medical records of 58 patients diagnosed with traumatic hyphema at Tangdu Hospital, Air Force Medical University, from January 2017 to December 2022. Factors such as age, sex, cause of injury, time since injury, initial visual acuity, hyphema grade, intraocular pressure, and complications were evaluated using multivariate logistic regression with SPSS software.
Results: The patient cohort was predominantly male (81%), with the majority of injuries occurring during occupational activities (38%). Other significant causes included altercations (19%), recreational activities (19%), traffic accidents (8.6%), sports injuries (8.6%), and fireworks (6.8%). Poor vision outcomes were primarily linked to vitreous hemorrhage, traumatic cataract, and iridodialysis, with significant risk factors being the time since injury, initial visual acuity, and hyphema grade.
Conclusions: Traumatic hyphema is most common in males and frequently results from occupational incidents. The most critical predictors of adverse visual outcomes are the grade of hyphema, initial visual acuity, and elapsed time since injury, with initial visual acuity serving as an independent predictor of vision impairment.
Trial registration: Not applicable. This retrospective study did not require trial registration.
Analogue self-interference cancellation of co-frequency co-time unidirectional transponders for low earth orbit satellite systems
Authors: Guo ShuoShuo , Ou Gang , Yu Xiaoyou , Lin Honglei , Huang Long , Li Pengpeng 
Published: May 2024

Abstract

In the era of beyond 5G and 6G, the application of low earth orbit (LEO) satellites and space Internet techniques has become a hot topic of current research. The future trend in this domain is to integrate the communication, navigation, and remote sensing functions to build a satellite–ground integration network. One of the advantages is that it will greatly benefit from making up for the shortage of traditional terrestrial cellular network coverage and the precision, convergence time, and signal strength that users receive from high-orbit navigation satellites. However, developing such a versatile, sophisticated, and intelligent platform is still challenging. The main issue arises when the low earth orbit satellites, work as transponders and communicate with high orbit navigation satellites and terminal receivers on the ground in a co-frequency co-time unidirectional transponder (CCUT) leading to severe self-interference. what’s worse, the power augmentation signal sent to the GNSS receiver is far stronger than the navigation augmentation signal received from a high-orbit satellite, which leads to receiver saturation. In this paper, a self-interference cancellation algorithm has been realized on an embedded platform. Experimental results show that the cancellation depth at the navigation frequency band with 10.23MHz bandwidth was 30dB.
Genetics of environmental sensitivity to psychiatric and neurodevelopmental phenotypes: evidence from GWAS of monozygotic twins
Authors: Assary Elham , Coleman Jonathan , Hemani Gibran , Veijer Margot van Der , Howe Laurence , Palviainen Teemu , Grasby Katrina , Ahlskog Rafael , Nygaard Marianne , Cheesman Rosa , Lim Kai , Reynolds Chandra , Ordoñana Juan , Colodro-Conde Lucia , Gordon Scott , Madrid-Valero Juan , Thalamuthu Anbupalam , Hottenga Jouke-Jan , Mengel-From Jonas , Armstrong Nicola J. , Sachdev Perminder , Lee Teresa , Brodaty Henry , Trollor Julian , Wright Margaret , Ames David , Catts Vibeke , Latvala Antti , Vuoksimaa Eero , Mallard Travis , Harden K , Tucker-Drob Elliot , Oskarsson Sven , Hammond Christopher , Christensen Kaare , Taylor Mark , Lundström Sebastian , Larsson Henrik , Karlsson Robert , Pedersen Nancy , Mather Karen , Medland Sarah , Boomsma D , Martin Nicholas , Plomin Robert , Bartels Meike , Lichtenstein Paul , Kaprio Jaakko , Eley Thalia , Davies Neil , Munroe Patricia , Keers Robert 
Published: May 2024

Abstract

Individual sensitivity to environmental exposures may be genetically influenced. This genotype-by-environment interplay implies differences in phenotypic variance across genotypes. However, environmental sensitivity genetic variants have proven challenging to detect. GWAS of monozygotic twin differences is a family-based variance analysis method, which is more robust to systemic biases that impact population-based methods. We combined data from up to 21,792 monozygotic twins (10,896 pairs) from 11 studies to conduct the largest GWAS meta-analysis of monozygotic phenotypic differences in children and adolescents/adults for seven psychiatric and neurodevelopmental phenotypes: attention deficit hyperactivity disorder (ADHD) symptoms, autistic traits, anxiety and depression symptoms, psychotic-like experiences, neuroticism, and wellbeing. The SNP-heritability of variance in these phenotypes were estimated (h2: 0% to 18%), but were imprecise. We identified a total of 13 genome-wide significant associations (SNP, gene, and gene-set), including genes related to stress-reactivity for depression, growth factor-related genes for autistic traits and catecholamine uptake-related genes for psychotic-like experiences. Monozygotic twins are an important new source of evidence about the genetics of environmental sensitivity.
Impact of ESG practices on European Bank Lending for Sustainability: The Role of Culture and Institutions
Authors: Shabir Mohsin , Işık Özcan , Hashmi Shujahat Haider , Mujtaba Ghulam 
Published: May 2024

Abstract

This study investigates the impact of Environmental, Social and Governance (ESG) practices on bank lending in the European Union banking sector, using a sample of 53 banks from 19 European Union countries for the period 2004–2018. Our findings show that ESG activities play a significant role in increasing bank lending. Interestingly, the ESG pillars follow a different pattern. Environmental and governance-friendly activities impact bank lending more than social. Moreover, the national culture and institutional quality significantly affect the ESG activity and bank lending nexus. Our outcomes demonstrate that national cultural dimensions significantly impact bank lending and mediate the relationship between ESG activity and bank lending. In comparison, good quality institutional supports banks in increasing their lending capacity and enhancing the ESG practice's constructive impact. The empirical findings are quite robust to using the various ESG dimensions, alternative estimation techniques, and control for the regulatory environment and macroeconomic conditions.
JEL Classification: G20, G21
Comparative study of Sombor index and its various versions using regression models for top priority polycyclic aromatic hydrocarbons
Authors: B. Kirana , M.C. Shanmukha , A. Usha 
Published: May 2024

Abstract

The aromatic compounds having structural configurations with two or more fused benzene rings are the polycyclic aromatic hydrocarbons(PAHs). Topological indices are valuable tools for studying the structure property relationships of PAHs and also helps in predicting various properties and activities. They find applications widely in computational chemistry, drug design and QSAR studies. This article focuses on analysing the potential predictive index for Sombor index( SO ), elliptic Sombor index(ESO ), Euler Sombor index(EU ), Reverse Sombor index(RSO), Reverse elliptic Sombor index(RESO ) and Reverse Euler Sombor index(REU ) using regression models for top priority 38 PAHs. From the study it is evident that, SO and RSO have proved to be potential predictive indices among the considered degree-based and reverse degree-based indices. The variation of best predictive index with minimal RMSE are plotted for linear, quadratic and cubic regression models for better understanding.
Maternal exposure to legacy PFAS compounds PFOA and PFOS is associated with disrupted cytokine homeostasis in neonates: the Upstate KIDS Study (2008-2010)
Authors: Jones Laura E. , Bell Erin 
Published: May 2024

Abstract

Background. Numerous studies suggest exposure to the environmentally ubiquitous legacy per/polyfluoroalkyl (PFAS) compounds perfluorooctane sulfonate (PFOS) and perfluorooctanoic acid (PFOA) may be associated with suppressed immune response, including attenuated vaccine-antibody response in children and greater susceptibility to opportunistic infections in general adult populations. We examined associations between neonatal concentrations of legacy PFAS compounds PFOA and PFOS and neonatal cytokine profiles from a large sample of residual newborn dried blood spots (NBDS) in upstate New York.
Methods. We measured 30 common cytokines along with PFOA and PFOS in eluted samples of newborn dried blood spots (NDBS) from 3448 neonates participating in the Upstate KIDs Study (2008-2010), following parental consent. We performed adjusted mixed effects regressions for each cytokine against PFAS species, testing for effect modification by infant sex. We then performed exploratory factor analysis (EFA) on PFAS species-specific cytokine subsets selected via the prior regressions, extracting 4 factor axes for the PFOA cytokine subset and 3 for the PFOS cytokine subset based on results from cluster analysis and parallel analysis. Regressions on each PFAS-specific set of factors followed. All models were adjusted for infant birth weight and gestational age at birth, maternal age, race, and use of fertility treatment, and included a random intercept to account for twins.
Results. Significant cytokine profiles were dominated by cytokines negatively associated with the given PFAS (9 of 11 cytokines for PFOA; 8 of 11 for PFOS). Regression by PFAS quartile shows evidence of nonlinearity in dose-response for most cytokines. All significant associations between factor groupings defined by EFA are negative for both PFOA and PFOS.
Conclusions. There is strong evidence that PFOA and PFOS exposures are associated with disrupted, typically reduced, cytokine levels, both singly and as functional groups defined by EFA and cluster analysis.
Recent Synthesis of Nucleoside Phosphonate Analogs Using Olefin
Cross-metathesis
Authors: Hong Joon Hee 
Published: May 2024

Abstract:
Nucleotide analogs known as acyclic and cyclic nucleoside phosphonates
(ANPs and CNPs, respectively) have a variety of biological properties, including antibacterial, antiviral, antiparasitic, antineoplastic, and immunomodulatory. A strong reaction
that has emerged in the last several decades has fundamentally changed our knowledge of
the chemistry of nucleoside phosphonates. In particular, Olefin cross-metathesis (CM) has
been a potent and practical synthesis route to produce functionalized olefins from essential
alkene precursors. This review describes recent synthesis examples of ANPs and CNPs
analogs using the Ru-catalyzed olefin cross-metathesis reactions. Olefin cross-metathesis
reactions are performed in the olefinic parts of nucleoside and phosphonate produced by
Grubbs, Hoveyda-Grubbs, and Nolan. This review presents a synthetic overview of a few
chosen nucleosides with biological significance. Their biological activity results are briefly discussed.
Applications of Inorganic Nanomaterials against Tuberculosis: A Comprehensive Review
Authors: Dastidar Debabrata Ghosh , Roy Arnab , Ghosh Gourav , Mandal Supratim 
Published: May 2024

Abstract:
Tuberculosis (TB) continues to pose a significant global health threat, with millions of
new infections recorded annually. Current treatment strategies, such as Directly Observed Treatment
(DOT), face challenges, including patient non-compliance and the emergence of drug-resistant
TB strains. In response to these obstacles, innovative approaches utilizing inorganic/metallic
nanomaterials have been developed to enhance drug delivery to target alveolar macrophages,
where Mycobacterium tuberculosis resides. These nanomaterials have shown effectiveness against
various strains of TB, offering benefits such as improved drug efficacy, minimized side effects,
and sustained drug release at the infection site. This comprehensive review explores the applications
of different metal nanoparticles, metal oxide nanoparticles, and metal-metal oxide hybrid
nanoparticles in the management of TB, including multidrug-resistant (MDR) and extensively
drug-resistant (XDR) strains. The synergistic effects of combining inorganic nanoparticles with
conventional anti-TB drugs have demonstrated promising results in combating TB infections. Further
research and development in this field hold great promise for overcoming the challenges
faced in current TB therapy and improving patient outcomes.