bluuebunny/crossref_metadata_2025_split huggingface dataset:
Coexistence of Sjögren's syndrome and sarcoidosis: a report of five cases
Authors: Mansour M. J. , Al‐Hashimi I. , Wright J. M. 
Published: August 2010

Background:  Sjögren's syndrome (SS) and sarcoidosis are diseases that can affect the salivary glands and result in the loss of salivary gland function. Most of the criteria used for the diagnosis of SS exclude sarcoidosis before establishing the diagnosis of SS. However, several reports have suggested the coexistence of both SS and sarcoidosis in the same patient.Objective:  The purpose of this study was to present five cases that support a true coexistence of sarcoidosis and SS.Methods:  Clinical and laboratory findings of patients with evidence of having both SS and sarcoidosis were reviewed. The diagnosis of SS was based on the European community criteria; the diagnosis of sarcoidosis was based on the presence of serological, radiographic and/or histopathologic findings that are consistent with sarcoidosis.Results:  All patients fulfilled the criteria for the diagnosis of both diseases.Conclusion:  Our findings appear to support a true coexistence of sarcoidosis with SS. Therefore, it is reasonable to suggest removing the exclusion of sarcoidosis from the diagnostic criteria for SS.
Prevalence of oral Candida species in leprosy patients from Cambodia and Thailand
Authors: Reichart P. A. , Samaranayake L. P. , Bendick Ch. , Schmidt‐Westhausen A. M. , Jayatilake J. A. M. S. 
Published: August 2010

Background:  Leprosy is a chronic bacterial infection which may lead to significant orofacial morbidity. However, reports on the oral mycotic flora of leprosy patients are rare. The aim of the current study was to explore the oral yeast carriage in two groups of leprosy patients.Methods:  40 Cambodian (seven men, 33 women) and 48 Thai (14 men, 34 women) leprosy patients from Leprosy Rehabilitation Centre Khien Kleang, Phnom Penh, Cambodia and McKean Rehabilitation Center, Chiangmai, Thailand were randomly selected and their demographic data and clinical history were recorded. Tongue and palatal swabs of each patient were collected using sterile Fungi‐Quick swabs (Hain Diagnostika, Nehren, Germany) and they were cultured aerobically on Sabouraud's dextrose agar and CHROMAgar (CHROMagar, Paris, France). Yeast were identified by germ tube, chlamydospore production, and assimilation tests (API 20C AUX, Bio‐Merieux, Marcy l'Etoile, France) and reconfirmed using APILAB Plus system (Bio‐Merieux).Results:  Two groups (Cambodian and Thai) had median age of 35 and 64 years. They had been with leprosy for median durations of 17.7 and 38.9 years (P < 0.05), respectively. Overall yeast carriage in two cohorts were 80% and 93.75%. Candida albicans had highest carriage rate in either group (65.6%, 44.4%). Candida krusei and C. glabrata existed as second‐line colonizers after C. albicans. Candida glabrata carriage was significantly higher in Thai patients (P < 0.05). Multispecies carriage was seen in three Cambodian (9.4%) and five Thai (11.5%) patients.Conclusions:  This study indicates high oral yeast carriage in leprosy patients. Candida albicans remains predominant while C. krusei and C. glabrata are second‐line oral colonizers. Co‐inhabitation of multiple yeast species is also noted in these patients’ oral mycotic flora.
Syndecan‐1 and Wingless‐type protein‐1 in human ameloblastomas
Authors: Leocata P. , Villari D. , Fazzari C. , Lentini M. , Fortunato C. , Nicòtina P. A. 
Published: August 2010

Background:  Aberrant Wingless type 1 glycoprotein (Wnt) pathway in ameloblastomas and a role of syndecan‐1 (SDC1) in activating Wnt signalling were perspected. SDC1 shifting from epithelium to stroma was reported in invasive non‐odontogenic neoplasms. The aim of this study was to reveal the role of SDC1 and Wnt1 in intraosseous ameloblastomas (IAs).Methods:  SDC1 and Wnt1 expressions were investigated in 29 ameloblastoma subtypes and seven tooth buds.Results:  SDC1 immunostaining strongly depicted stromal cells, extracellular matrix (ECM) and basement membranes of ameloblastomas. It also showed epithelial tumour cells in the acanthomatous and plexiform subtypes, and it often occurred in stellate reticulum cells and basal ameloblasts of tooth buds. Parallel Wnt1 expression occurred in ameloblastomatous epithelial cells, but it was common in basal cells of tooth buds too. Statistically, a significant correlation was found between the percentage of IAs‐bearing SDC1‐positive stromal cells and ECM and the percentage of IAs‐bearing Wnt1‐positive epithelial cells.Conclusions:  A role of SDC1 in stromal cells and ECM can be hypothesized as a critical factor for carcinogenesis and local invasiveness of IAs.
An association between the MICA‐A5.1 allele and an increased susceptibility to oral squamous cell carcinoma in Japanese patients
Authors: Tamaki Shigehiro , Sanefuzi Nobuyuki , Ohgi Kazuhiko , Imai Yuichiro , Kawakami Masayoshi , Yamamoto Kazuhiko , Ishitani Akiko , Hatake Katsuhiko , Kirita Tadaaki 
Published: August 2010

Background:  Recently, a new polymorphic gene family called the major histocompatibility complex class I chain‐related gene A (MICA) was discovered about 40 kb centromeric to HLA‐B gene. The MICA protein, expressed on epithelial cells and many kinds of tumor cells, serves to regulate immune function. The MICA protein is thought to activate immune function on mucosal tissue by binding to NKG2D which is expressed on most natural killer cells, CD8 positive T cells, and gamma delta T cells. An association between MICA gene polymorpisms and the development of oral squamous cell carcinoma (OSCC) has also been reported.Objective:  This study was designed to test this association in Japanese patients with OSCC.Methods:  The (GCT)n polymorphisms of the MICA gene was investigated in 123 patients with OSCC and 188 normal controls using polymerase chain reaction amplification and denaturing polyacrylamide gel electrophoresis.Results:  Five alleles, namely A4, A5, A6, A9, and A5.1, were found in both groups. The phenotype frequency of the MICA‐A5.1 allele was significantly higher in patients with OSCC when compared with normal controls (OR 1.707, 95% CI 0.76–3.45, P = 0.042). Also, the microsatellite frequency of the MICA‐A5.1 allele was significantly higher in patients with OSCC compared with normal controls (OR 1.664, 95% CI 0.82–3.42, P = 0.021). Lastly, the frequency of the MICA‐A5.1 allele was significantly higher in those with lymph node metastasis from OSCC compared with normal controls (OR 2.605, 95% CI 1.14–5.27, P = 0.026).Conclusions:  These results suggest that the MICA‐A5.1 allele may be associated with an increased susceptibility to OSCC in Japan.
Analysis of histopathological and immunohistochemical differences of oral squamous cell carcinoma in young and old patients in Sri Lanka
Authors: Siriwardena B. S. M. S. , Tilakaratne A. , Amaratunga E. A. P. D. , Udagama M. N. G. P. K. , Ogawa Ikuko , Kudo Yasusei , Takata Takashi , Tilakaratne W. M. 
Published: August 2010

Background:  Oral squamous cell carcinoma (OSCC) most commonly occurs in the middle‐aged and older individuals. The purpose of the present study was to evaluate the histopathological and immunohistochemical differences of the younger (<40 years) and the older (more than 50 years) groups.Methods:  The histopathological parameters of 112 patients (younger 56 and older 56) were compared according to three grading systems (Broder JAMA 1920; 74: 656, Anneroth et al. Scand J Dent Res 1987; 95: 229, Bryne et al. J Pathol 1992; 166: 375) and as individual histopathological parameters. Further, the expression of p53 and Proliferative Cell Nuclear Antigen (PCNA) index was also compared.Results:  Although there was no significant difference between two groups regarding the three grading systems, a significantly higher number of nuclear aberrations was found in younger group (P < 0.001). Interestingly, higher number of mitoses (P < 0.05) and lymph node metastasis (P < 0.05) were observed in the older group (P < 0.05). Furthermore, significantly a higher PCNA index was found in the older group (P < 0.005).Conclusions:  Although tumours of the young patients showed more nuclear aberrations, OSCC of the older patients is proliferative and showed higher metastatic rate.
Additional use of DNA‐image cytometry improves the assessment of resection margins
Authors: Handschel Jörg , Öz Döne , Pomjanski Natalia , Depprich Rita , Ommerborn Michelle A. , Braunstein Stefan , Kübler Norbert R. , Meyer Ulrich , Böcking Alfred 
Published: August 2010

Background:  Despite the histopathologic findings of tumor‐free margins, patients with oral squamous cell carcinoma (SCC) often suffer from local tumor relapse. The purpose of this study was to determine the prognostic value of DNA‐image cytometry in the assessment of resection margins.Methods:  DNA‐image cytometry was performed in 40 SCC patients with histologically tumor‐free resection margins. The follow‐up period since the tumor resection was at least 3 years.Results:  Twenty patients showed a locoregional relapse of the SCC. Fourteen of these patients had aneuploid cells in DNA‐image cytometry. Two patients who were relapse‐free revealed aneuploid cells too. The sensitivity of the adjuvant use of DNA‐image cytometry was 70% and the positive predictive value was 87.5%.Conclusions:  The additional use of DNA‐image cytometry is a reasonable tool for the assessment of the resection margins of SCCs. DNA‐image cytometry could help to find the appropriate treatment option for the patients and thus might improve their prognosis.
Oral manifestations of systemic and cutaneous lupus erythematosus in a Venezuelan population
Authors: López‐Labady Jeaneth , Villarroel‐Dorrego Mariana , González Nieves , Pérez Ricardo , Mata de Henning Magdalena 
Published: August 2010

Background:  The aim of this study was to characterize oral lesions in patients with systemic and cutaneous lupus erythematosus (LE) in a Venezuelan group.Methods:  Ninety patients with LE were studied. Oral biopsies were taken from patients who showed oral mucosal involvement. Tissue samples were investigated with histology and direct immunofluorescence techniques for the presence of immunoglobulins G, M, A and complement factor C3.Results:  In 90 patients with LE, 10 patients showed oral lesions related to the disease. Sixteen lesions were investigated. Oral ulcerations accompanied by white irradiating striae occurred in five patients, erythema was observed in five patients and a white homogeneous plaque in one patient. Fifteen lesions demonstrated vacuolar basal degeneration and 12 thickening of the basement membrane histologically. Direct immunofluorescence was negative in three samples.Conclusions:  These findings corroborated that ulcers are not the only manifestation of LE in the oral mucosa. Clinical and histological examinations are significant as immunoproteins are not always found on the oral sample.
Epigallocatechin‐3‐gallate inhibits the invasion of human oral cancer cells and decreases the productions of matrix metalloproteinases and urokinase‐plasminogen activator
Authors: Ho Yung‐Chuan , Yang Shun‐Fa , Peng Chih‐Yu , Chou Ming‐Yung , Chang Yu‐Chao 
Published: August 2010

Background:  Green tea polyphenols are considered beneficial to human health, especially as cancer chemopreventive agents in recent years. Epigallocatechin‐ 3‐gallate (EGCG), the most abundant polyphenol in green tea, has been proven to suppress colonic tumorigenesis in animal and epidemiological studies, whereas its role in the oral carcinogenesis remains to be elucidated.Methods:  Cytotoxicity, invasion, and migration assays were used to investigate the effects of human oral cancer cell line OC2 cells exposed to EGCG. To look at the precise involvement of EGCG in cancer metastasis, gelatin zymography and casein zymography were performed to evaluate the impacts of EGCG on matrix metalloproteinase (MMP)‐2, MMP‐9, and urokinase plasminogen activator (uPA) secretion in OC2 cells.Results:  EGCG exhibited a dose‐dependent inhibitory effect on the invasion and migration of OC2 cells in the absence of cytotoxicity (P < 0.05). EGCG was also found to decrease the expressions of MMP‐2, MMP‐9, and uPA in a concentration‐dependent manner (P < 0.05).Conclusion:  Taken together, these results suggest that EGCG could inhibit the invasion and migration of human oral cancer cells and that the effects may partially because of the decreased productions of MMP‐2, MMP‐9, and uPA.
Recurrent aphthous stomatitis revisited; clinical features, associations, and new association with infant feeding practices?
Authors: McCullough Michael John , Abdel‐Hafeth Samah , Scully Crispian 
Published: August 2010

Background:  Recurrent aphthous stomatitis (RAS) is a common oral mucosal disease, characterized by painful oral ulcerations whose causes is poorly understood. The aim of the present study was to assess the characteristics of patients suffering from RAS.Methods:  Five hundred and twenty‐eight consecutive patients attending an Oral Medicine Specialist Clinic in the UK were examined and 143 specific attributes recorded.Results:  Patients with a clinical diagnosis of RAS were significantly more often males (P = 0.001) younger (P < 0.001). Significant correlations were observed between a diagnosis of RAS and trauma (P = 0.044), stress (P = 0.006), non‐smoking (P < 0.001), a family history (P < 0.001), breast feeding (P = 0.017) and the site in the mouth (buccal, labial, floor of mouth) (P < 0.007).Conclusion:  This retrospective analysis of patients attending a tertiary referral center provides evidence to support some long held beliefs about RAS, such as young age of onset, sites affected, and associations with trauma, stress, familial history and hormonal changes, while raising a new and interesting decreased incidence with breast feeding.
ORIGINAL ARTICLE: Lack of Consistent Association Between Endothelial Nitric Oxide Synthase Gene Polymorphisms, Homocysteine Levels and Recurrent Pregnancy Loss in Tunisian Women
Authors: Zammiti Walid , Mtiraoui Nabil , Mahjoub Touhami 
Published: August 2010

Problem  Polymorphisms of the endothelial nitric oxide synthase (eNOS) gene have been associated with reduced vascular NO production or increased level of homocysteine, and evaluated as risk factors for recurrent pregnancy loss (RPL). Therefore, in this case‐control study, we aimed to determine the effects of some eNOS functional polymorphisms: the 27‐bp intron 4 repeat, the 894G/T of exon 7, and the promoter substitution ‐786T/C, in women with RPL.Method of study  We genotyped 350 patients with RPL and 200 healthy women by polymerase chain reaction (PCR) and restriction fragment length polymorphism‐PCR (RFLP‐PCR). The homocysteine total plasma concentrations (tHcy) were determined by enzyme‐linked immunosorbent assay (ELISA).Results  None of the eNOS polymorphisms‐related alleles, genotypes, and haplotypes were associated with RPL. The tHcy were similar between patients and controls; no significant association between tHcy levels and eNOS genotypes could be evidenced.Conclusion  The present study identified a lack of association between eNOS gene polymorphisms, the risk of RPL and tHcy levels.
ORIGINAL ARTICLE: Follicular Fluid Levels of Interleukin‐10 and Interferon‐gamma do not Predict Outcome of Assisted Reproductive Technologies
Authors: Cerkiene Zivile , Eidukaite Audrone , Usoniene Audrone 
Published: August 2010

Problem  Follicular fluid (FF) and surrounding tissue contains various lymphocytes that synthesize different cytokines. The other sources of cytokines are ovarian somatic cells. FF provides microenvironment for the oocyte and contains immunological factors for the regulation of its development. Changes in expression and concentrations of certain cytokines can influence oocyte and embryo quality, resulting in a reduced ability to implant. Some data shows that follicular environment depends on infertility indication. The purpose of our study was to investigate whether cytokines interleukin‐10 (IL‐10) and interferon‐γ (IFN‐γ) are released in the human FF and also to evaluate impact of those cytokines on fertilization rate, cleavage rate, embryo quality, and pregnancy rate.Methods of study  Couples treated by assisted reproductive technologies were selected for this study. A total of 121 patients participated in the study. Until cytokine detection samples of FF were stored at −20°C. In vitro fertilization or intracytoplasmic sperm injection procedure was used depending on the indication. After 72 hr, on the day of transfer, embryo morphology was evaluated. Embryo transfer based on embryo morphology was performed. Commercial enzyme‐linked immunosorbent assay kit from Diaclone, France was used for the quantitative determination of human IL‐10 and IFN‐γ concentrations in FF. Analysis of the results was performed using spss 12.0.Results  One hundred and twenty‐one FF samples were tested for IL‐10 and IFN‐γ. IFN‐γ and IL‐10 were detected in 14 (11.6%) and 108 (89.3%) FF samples, respectively (range 0.9–31.1 pg/mL for IFN‐γ and 0.7–10.8 pg/mL for IL‐10). There was no significant correlation between infertility indication, fertilization rate, cleavage rate, and concentrations of the follicular cytokines. Similarly, IL‐10 concentrations did not differ significantly in different age groups and did not alter pregnancy rate.Conclusion  Our study showed that IFN‐γ and IL‐10 are not suitable markers in predicting outcome of assisted reproductive technologies.
ORIGINAL ARTICLE: Prenatal Priming of Cord Blood T Lymphocytes by Microbiota in the Maternal Vagina
Authors: Stencel‐Gabriel Krystyna , Gabriel Iwona , Wiczkowski Andrzej , Paul Marek , Olejek Anita 
Published: August 2010

Problem  In the vagina of women at the reproductive age, more than 170 strains of bacteria and yeasts are found. The effect of vaginal flora on neonatal T cells is yet to be investigated.Method of study  We analyzed CD45RA and CD45RO expression on neonatal CD4+ T cells and cytokine production in CBMC cultures (interferon‐γ (IFN‐γ ), interleukin‐4 (IL‐4) and IL‐12) related to vaginal bacteria isolated from a maternal vagina. We collected vaginal swabs from 36 women at the first stage of the delivery and cord blood from their newborns. IFN‐γ, IL‐4, and IL‐12 in stimulated CBMC were measured and the expression of CD45RA/CD45RO on CD4+ T cells was assessed.Results  We noted the difference in CD45RO CD4+ expression and IL‐12 levels between the newborns whose mothers were or were not colonized with Lactobacillus in the vagina (newborns whose mothers were colonized with Lactobacillus: CD45RO‐10%±3; IL‐12‐0.2 pg/mL ± 0.05; newborns whose mothers were not colonized with Lactobacillus: CD45RO‐6%±3; IL‐12‐2.0 pg/mL ± 0.7).Conclusion  Our results may indicate that lactobacilli in maternal vagina influence the development of neonatal immune system. Yet, more research is needed using specified bacterial antigens.
ORIGINAL ARTICLE: The Role of TSP‐1 on Decidual Macrophages Involved in the Susceptibility to Unexplained Recurrent Spontaneous Abortion
Authors: Jin Yan , Wang Xipeng , Xiao Yunshang , Lv Caijun , Ding Chuanwei , Lin Qide 
Published: August 2010

Problem  To investigate the role of TSP‐1 on decidual macrophages (DMΦ) in unexplained recurrent spontaneous abortion (RSA).Method of study  A total of 20 women undergoing artificial abortion in the first trimester and 10 patients with RSA were selected. (i) The expression of TSP‐1 mRNA in deciduas was detected by real‐time polymerase chain reaction; (ii) Flow cytometry was used to detect the percentage of positive TSP‐1, CD36, CD47 markers on macrophages. (iii) Cytokine expression was measured by enzyme‐linked immunosorbent spot‐forming (ELISPOT) cell assay.Results  (i) The expression of TSP‐1 mRNA on DMΦ in RSA was significantly decreased (P < 0.05). (ii) The increased expression of CD36 (P < 0.01) and the decreased expression of TSP1 (P < 0.01) were found on DMΦ of RSA. No different CD47 expression level on DMΦ was observed between two groups. (iii)The expression of IL‐10 in DMΦ of RSA patients was decreased significantly compared with that of controls (P < 0.05). When adding TSP1 into culture medium, there was no change in IFN‐γ expression, but increased IL‐10 (P < 0.05) expression in DMΦ of RSA patients was observed. The expression of IL‐10 was decreased significantly in DMΦ from controls when adding anti‐TSP‐1 antibody to culture medium (P < 0.05).Conclusion  TSP‐1 on DMΦ could influence IL‐10 expression as Th2 cytokines. The abnormal expression of TSP‐1 could make some women undergo pregnancy loss.
Reliability of the SNAP (score of neonatal acute p00hysiology) data collection in mechanically ventilated term babies in New South Wales, Australia
Authors: Sutton L , Bajuk B , Berry G , Sayer GP , Eagles BL , Henderson‐Smart DJ 
Published: August 2010

The aim of this population‐based, case‐control, cohort study was to report inter‐rater reliability between the New South Wales Neonatal Intensive Care Unit Data Collection (NICUS) audit nurses' collection of SNAP (OS) and a research nurse's SNAP data as the audit SNAP (AS). The study was carried out in Sydney and four large rural/urban health areas in New South Wales (NSW), Australia. The subjects—182 singleton term infants with no major congenital anomalies—were admitted to a tertiary neonatal intensive care unit (NICU) for mechanical ventilation. SNAP data were collected on the 182 case infants, born between 1 January and 31 December 1996, by clinical audit officers in the nine tertiary NICUs in NSW. The research officer conducted an audit of the original SNAP score on all infants. The data were examined using Pearson's correlation coefficient, weighted kappa, a plot of difference in SNAP against mean SNAP and Wilcoxon's signed rank sum test. Pearson's correlation coefficient between the OS and AS data was 0.80. Median (interquartile range) SNAP was 13 (9,19) for the OS and 14 (10,20) for the AS. Weighted kappa was highest for highest heart rate, paO2, temperature (°C), oxygenation index, haematocrit, platelet count, lowest serum sodium, lowest blood glucose and seizure. In 17 (9%) infants, OS and AS differed by ≥10, 14 because of an original data collection error, 1 data entry error, 1 audit error and 1 for both data collection and data entry errors. Conclusion: If SNAP is to be incorporated into any routine NICU data collection, it should be audited regularly on a sample of records. It is important to standardize and adhere to strict definitions for parameters before the collection of SNAP data.
Continuous gastric drip versus intravenous fluids in low birthweight infants
Authors: Becerra M , Cifuentes J , Saldías MI , Gálvez MC , Fernández P , Aguila A 
Published: August 2010

This multicentre randomized study compared a continuous gastric drip (CGD) with intravenous (i.v.) fluid administration. Healthy newborns with birthweight from 1501 to 2000 g whose physician ordered i.v. fluids were randomized before the 2nd hour of life to CGD or i.v. fluids. The major outcome variable was the need for an i.v. line in the CGD group. Serum glucose was measured at 30 min, 1 h and every 6 h thereafter. Serum sodium and potassium were measured at least once during the first 72 h of life. Enteral feedings, feeding intolerance, number of venous lines and i.v. line‐related complications were recorded until the interruption of CGD or the i.v. line. Twenty‐nine infants were randomized to each group. The two groups were comparable in terms of birthweight and gestational age. Ten percent (3/29) of the infants randomized to the CGD group required i.v. fluids and 90% of them received electrolytes and glucose through an orogastric tube. The incidence of hypoglycaemia, hyponatraemia and episodes of feeding intolerance did not differ between the groups. Conclusion: Fluid administration by CGD reduces the need for i.v. lines without increasing the risk of complications.