bluuebunny/crossref_metadata_2025_split huggingface dataset:
Selective Synthesis of a Hexagonal Co(II)‐Substituted Sodium Zincophosphate via a Simple and Novel Route
Authors: Liao Sen , Liu Gang , Tian Xiaozhen , Chen Xia , Wu Wenwei , Liang Yugui 
Published: February 2010

AbstractA simple and novel route was provided for the preparation of the hexagonal Co(II)‐substituted sodium zincophosphate (CoZnPO‐HEX), which was obtained with ZnSO4·7H2O, CoCl2·6H2O and Na3PO4·12H2O as raw materials and polyethylene glycol‐400 (PEG‐400) as a surfactant via a one‐step solid‐state reaction at low heating temperature (60°C), and characterized with X‐ray diffraction (XRD), fourier transform infrared spectroscopy (FTIR), diffuse reflectance spectra, thermogravimetric analysis and the 1st derivative of thermogravimetric analysis (TG/DTG) and transmission electron microscopy (TEM). The experimental results showed that the CoZnPO‐HEX was selectively formed via this reaction at ambient temperature in the presence of PEG‐400.
Chirality Control by Substituents in the Asymmetric Addition of Et2Zn to Aromatic Aldehydes Catalyzed by cis‐(1R,2S)‐2‐Benzamidocyclohexanecarboxylic Acid Derived 1,3‐Aminoalcohols
Authors: Wang Xiangbo , Kodama Koichi , Hirose Takuji , Zhang Guangyou 
Published: February 2010

AbstractA series of novel optically active 1,3‐aminoalcohols based on cis‐(1R,2S)‐2‐benzamidocyclohexanecarboxylic acid and trans‐(1R,2R)‐2‐benzamidocyclohexanecarboxylic acid were synthesized and used in the asymmetric diethylzinc addition to aromatic aldehydes. Not only the enantioselectivity but also the stereochemistry of the product were controlled by the N‐substituents and the substituents on the vicinity carbon to hydroxyl group of the cis‐derivatives.
Ultrasound Promoted and Ionic Liquid Catalyzed Cyclocondensation Reaction for the Synthesis of 4(3H)‐Quinazolinones
Authors: Pawar Omprakash B. , Chavan Fulchand R. , Sakate Sachin S. , Shinde Narayan D. 
Published: February 2010

Abstract4(3H)‐Quinazolinones were synthesized in high yields by one‐pot three‐component condensation of anthranilic acid, carboxylic acid and aniline in the presence of ionic liquid such as 1‐n‐butyl‐3‐methylimidazolium tetrafluoroborate (BMImBF4) as catalyst under solvent free and neutral conditions.
Theoretical Study of One‐electron Redox Potentials of Some NADH Model Compounds
Authors: Wang Huajing , Huang Xiongyi , Shen Rong , Rui Lei , Fu Yao 
Published: February 2010

AbstractSeveral 1‐benzyl‐1,4‐dihydronicotinamide derivatives, which are important NADH model compounds were studied theoretically in acetonitrile. The performances of various DFT methods including B3LYP, B1B95, B3PW91, MPW1B95, MPWKCIS, and M06 were tested to calculate the redox potentials. The first theoretical protocol to predict the redox potentials of these derivatives is B1B95, whose reliability has been tested against almost all the available experimental data. Strikingly, the mean absolute derivations (MAD) and root mean square (RMS) error of the current theoretical model equal 0.015 and 0.017, respectively. By using this method, the important thermodynamic properties of BNAHs were investigated and the mechanisms of hydride transfer progress were explained. Besides, para‐substituents that have a big effect on these redox potentials of BNAH were systematically studied and carefully demonstrated.
Synthesis of N1‐Substituted 1,2,3,6‐Tetrahydropyrimidin‐ 2‐ones via an Unexpected Reaction of Thiazolo[3,2‐a]‐pyrimidine Derivatives and Nitrile Oxide
Authors: Li Xiaofang , Yi Pinggui , Yu Xianyong 
Published: February 2010

AbstractA new class of N1‐substituted 1,2,3,6‐tetrahydropyrimidin‐2‐ones was prepared in moderate yields by the reaction of nitrile oxide with thiazolo[3,2‐a]pyrimidine derivatives via a domino 1,3‐dipolar cycloaddition/ring‐opening/ substitution process. The structures of the products were characterized thoroughly by IR, elemental analysis, MS, NMR together with X‐ray crystallographic analysis.
A three‐dimensional particle simulation of the formation and collapse of a primary thrombus
Authors: Kamada Hiroki , Tsubota Ken‐ichi , Nakamura Masanori , Wada Shigeo , Ishikawa Takuji , Yamaguchi Takami 
Published: February 2010

AbstractThis report presents a technique based on the particle method to simulate the process of thrombogenesis while considering platelet aggregation under the influence of fluid dynamics. In the employed particle method, the blood region was discretized by particles that were assumed to have the characteristics of plasma and platelets. The moving particle semi‐implicit (MPS) method developed for incompressible viscous flow was applied to the flow of plasma and platelets. Adhesion of platelets to the injured vessel wall was expressed by a spring force acting between them. The same modeling was applied for the aggregation of platelets. Three‐dimensional computer simulation of thrombogenesis was performed in a rectangular flow channel under the condition of Re=0.02. We demonstrated that the proposed method can simulate the formation and destruction of a thrombus with the inclusion of feedback reactions of thrombus development and flow. The results revealed that the growth rate of a thrombus, its height, and time required from the beginning of thrombus formation to its collapse vary according to the flow rate, indicating that flow dynamics plays an important role in regulating the development of a primary thrombus. Copyright © 2010 John Wiley & Sons, Ltd.
The design of puzzle selection strategies for GWAP systems
Authors: Chen Ling‐Jyh , Wang Bo‐Chun , Chen Kuan‐Ta 
Published: February 2010

AbstractTheGames With A Purpose(GWAP) genre is a type ofHuman Computationthat outsources certain steps of the computational process to humans. By taking advantage of people's desire to be entertained, GWAP attracts people to play voluntarily, and also produce useful metadata as a by‐product. The games have shown promise in solving a variety of problems, which computer computation has been unable to resolve completely thus far. In this paper, we propose a metric, calledsystem gain, for evaluating the performance of GWAP systems, and also use analysis to study the properties of GWAP systems. We argue that it is important for GWAP systems to implement properpuzzle selection strategiesin order to collect human intelligence in a more efficient manner. Therefore, based on our analysis, we implement anOptimal Puzzle Selection Strategy(OPSA) to improve GWAP systems. Using a comprehensive set of simulations, we demonstrate that the proposed OPSA approach can effectively improve the system gain of GWAP systems, as long as the number of puzzles in the system is sufficiently large. Copyright © 2010 John Wiley & Sons, Ltd.
Management of a parameter sweep for scientific applications on cluster environments
Authors: Youn Choonhan , Kaiser Tim 
Published: February 2010

AbstractThe GEOsciences Network (GEON, www.geongrid.org) is a large‐scale collaborative cyberinfrastructure project involving information technology and geoscience researchers from multiple institutions. The GEON infrastructure provides portal, middleware, and data resources to facilitate scientific discovery for domain scientists using applications, tools, and services. It consists of both a service‐oriented Web/Grid framework and application toolkits, using the Web service and portlet programming model to represent applications. Based on those grid environments, we have developed the SYNSEIS (SYNthetic SEISmogram) tool within the GEON infrastructure to support personalized experiments in seismology. In this paper, we present an overview of SYNSEIS from a user point of view, and demonstrate how one can use a simple management scheme to perform a parameter sweep and distribute the work in computational resources, using a scientific application that was not specifically designed to perform parameter sweeps. The performance advantages to be gained by using this scheme with scientific codes for dealing with a large number of jobs on computational grids are very substantial. In particular, we identify the earthquake simulations in the SYNSEIS tool as an example application that can benefit from running jobs on computational resources and subsequently promote the sharing of computational resources among partner sites involved in the GEON project. Finally, we also discuss the parallel scaling behavior of our primary earthquake simulation application. Copyright © 2010 John Wiley & Sons, Ltd.
How Far Can We Go? Quantum‐Chemical Investigations of Oxidation State +IX
Authors: Himmel Daniel , Knapp Carsten , Patzschke Michael , Riedel Sebastian 
Published: February 2010

AbstractThe highest known oxidation state of any element is +VIII. After the recent discovery of IrVIIIO4 under cryogenic conditions, we have investigated the stability of cationic species [MO4]+ (M=Rh,Ir,Mt). Such compounds would formally represent the new oxidation state +IX, which is experimentally unknown so far for the whole periodic table. [IrO4]+ is predicted to be the most promising candidate. The calculated spin–orbit (SO) coupling shows only minor effects on the stability of the iridium species, whereas SO‐coupling increases enormously for the corresponding Eka‐Iridium (Meitnerium) complexes and destabilizes these.
Metal‐Free Heterogeneous Catalysis for Sustainable Chemistry
Authors: Su Dang Sheng , Zhang Jian , Frank Benjamin , Thomas Arne , Wang Xinchen , Paraknowitsch Jens , Schlögl Robert 
Published: February 2010

AbstractThe current established catalytic processes used in chemical industries use metals, in many cases precious metals, or metal oxides as catalysts. These are often energy‐consuming and not highly selective, wasting resources and producing greenhouse gases. Metal‐free heterogeneous catalysis using carbon or carbon nitride is an interesting alternative to some current industrialized chemical processes. Carbon and carbon nitride combine environmental acceptability with inexhaustible resources and allow a favorable management of energy with good thermal conductivity. Owing to lower reaction temperatures and increased selectivity, these catalysts could be candidates for green chemistry with low emission and an efficient use of the chemical feedstock. This Review highlights some recent promising activities and developments in heterogeneous catalysis using only carbon and carbon nitride as catalysts. The state‐of‐the‐art and future challenges of metal‐free heterogeneous catalysis are also discussed.
Parabiosis model does not show presence of circulating osteoprogenitor cells
Authors: Boban Ivana , Barisic‐Dujmovic Tatjana , Clark Stephen H. 
Published: February 2010

AbstractThe goal of this study was to determine the presence of osteoprogenitor cells in the peripheral blood. Experiments were conducted with a parabiosis model in which osteoblast specific transgenic mice (Col2.3GFP or hOC‐GFP) were surgically joined with a transgenic mouse where herpes virus thymidine kinase gene is under the control of the collagen α1 promoter (Col2.3ΔTK). This method permits conditional ablation of osteoblasts by ganciclovir (GCV) treatment. In parabionts treated with GCV for 15 days or 1.5–2 months, GFP (hOC‐GFP or Col2.3GFP) expression was not detected in histological preparations or in marrow stromal cell cultures from the Col2.3ΔTK parabiont. Finally, Col2.3GFP/Col2.3ΔTK pairs were treated with GCV for 15 days and allowed to recover from GCV for 3 months. Again there was a failure to detect Col2.3GFP expressing cells in the Col2.3ΔTK parabiont. These observations, at least within the limits of this model system, allow the conclusion that osteoprogenitor cells do not readily circulate. genesis 48:171–182, 2010. © 2010 wiley‐Liss, Inc.
A mouse line expressing Sall1‐driven inducible Cre recombinase in the kidney mesenchyme
Authors: Inoue Shuji , Inoue Miki , Fujimura Sayoko , Nishinakamura Ryuichi 
Published: February 2010

AbstractSall1 is expressed in the metanephric mesenchyme in the developing kidney, and mice deficient in Sall1 show kidney agenesis or dysgenesis. Sall1 is also expressed elsewhere, including in the limb buds, anus, heart, and central nervous system. Dominant‐negative mutations of Sall1 in mice and humans lead to developmental defects in these organs. Here, we generated a mouse line expressing tamoxifen‐inducible Cre recombinase (CreERT2) under the control of the endogenous Sall1 promoter. Upon tamoxifen treatment, these mice showed genomic recombination in the tissues where endogenous Sall1 is expressed. When CreERT2 mice were crossed with the floxed Sall1 allele, tamoxifen administration during gestation led to a significant decrease in Sall1 expression and small kidneys at birth, suggesting that Sall1 functions were disrupted. Furthermore, Sall1 expression in the kidney was significantly reduced by neonatal tamoxifen treatment. The Sall1CreERT2 mouse is a valuable tool for in vivo time‐dependent and region‐specific knockout and overexpression studies. genesis 48:207–212, 2010. © 2010 Wiley‐Liss, Inc.
Parenchymal cells critically curtail cytotoxic T‐cell responses by inducing Bim‐mediated apoptosis
Authors: Gruber Anton , Cannarile Michael A. , Cheminay Cedric , Ried Christine , Marconi Peggy , Häcker Georg , Brocker Thomas 
Published: February 2010

AbstractTo develop cytolytic effector functions, CD8+ T lymphocytes need to recognize specific Ag/MHC class I complexes in the context of costimuli on Ag‐presenting DC. Thereafter they differentiate into effector and memory CTL able to confer protection against pathogen infection. Using transgenic mice with DC‐selective MHC class I expression and DC‐specific versus ubiquitous vaccination regimen, we found that DC are sufficient to prime CTL responses. However, Ag recognition on parenchymal non‐professional APC negatively affected CD8+ T‐cell responses in mice by inducing expression of the pro‐apoptotic bcl2‐family member bim in CTL. This unexpected induction of apoptosis in the early phase of effector CTL accumulation lead to suboptimal clonal burst size and diminished long‐term memory. Thus, our data demonstrate that effector CTL differentiation and apoptosis are regulated independently. Moreover, Ag distribution on cells other than DC critically reduces CTL responses.
Targeted inhibition of IL‐10‐secreting CD25− Treg via p38 MAPK suppression in cancer immunotherapy
Authors: Ohkusu‐Tsukada Kozo , Toda Masahiro , Udono Heiichiro , Kawakami Yutaka , Takahashi Kimimasa 
Published: February 2010

AbstractCancer‐induced immunotolerance mediated by inducible Treg (iTreg) is a major obstacle to cancer immunotherapy. In a basic study of immunotolerance, injection of an endogenous superantigen, i.e. the minor lymphocyte stimulatory (Mls)‐1a, into specific TCR Vβ8.1‐Tg mice enabled generation of anergic CD25− iTreg, the immunosuppressive function of which was maintained by IL‐10 production via p38‐MAPK activation. Interestingly, although p38‐chemical inhibitor (p38‐inhibitor) is capable of breaking CD25− iTreg‐induced immunotolerance, the p38‐inhibitor had hardly any immunotolerance breaking effect when CD25+ Treg were present, suggesting that depletion of CD25+ Treg is necessary for p38‐inhibitor to be effective. Peptide OVA323–339 iv.‐injection into its specific TCR‐Tg (OT‐II) mice also induced adaptive tolerance by iTreg. Peptide immunotherapy with p38‐inhibitor after CD25+ Treg‐depletion was performed in an OVA‐expressing lymphoma E.G7‐bearing tolerant model established by adoptive transfer of OT‐II CD25− iTreg, which resulted in suppression of tumor growth. Similarly, the antitumor immunity induced by peptide immunotherapy in colon carcinoma CT26‐bearing mice, in which the number of IL‐10‐secreting iTreg is increased, was augmented by treatment with p38‐inhibitor after CD25+ Treg‐depletion and resulted in inhibition of tumor progression. These results suggest that simultaneous inhibition of two distinct Treg‐functions may be important to the success of cancer immunotherapy.
Impaired CD4+ T‐cell proliferation and effector function correlates with repressive histone methylation events in a mouse model of severe sepsis
Authors: Carson William F. , Cavassani Karen A. , Ito Toshihiro , Schaller Matthew , Ishii Makoto , Dou Yali , Kunkel Steven L. 
Published: February 2010

AbstractImmunosuppression following severe sepsis remains a significant human health concern, as long‐term morbidity and mortality rates of patients who have recovered from life‐threatening septic shock remain poor. Mouse models of severe sepsis indicate this immunosuppression may be partly due to alterations in myeloid cell function; however, the effect of severe sepsis on subsequent CD4+ T‐cell responses remains unclear. In the present study, CD4+ T cells from mice subjected to an experimental model of severe sepsis (cecal ligation and puncture (CLP)) were analyzed in vitro. CD4+CD62L+ T cells from CLP mice exhibited reduced proliferative capacity and altered gene expression. Additionally, CD4+CD62L+ T cells from CLP mice exhibit dysregulated cytokine production after in vitro skewing with exogenous cytokines, indicating a decreased capability of these cells to commit to either the TH1 or TH2 lineage. Repressive histone methylation marks were also evident at promoter regions for the TH1 cytokine IFN‐γ and the TH2 transcription factor GATA‐3 in naïve CD4+ T cells from CLP mice. These results provide evidence that CD4+ T‐cell subsets from post‐septic mice exhibit defects in activation and effector function, possibly due to chromatin remodeling proximal to genes involved in cytokine production or gene transcription.
SUMOylation attenuates c‐Maf‐dependent IL‐4 expression
Authors: Lin Bo‐Shiou , Tsai Pei‐Yun , Hsieh Wan‐Yun , Tsao Hsiao‐Wei , Liu Meng‐Wei , Grenningloh Roland , Wang Li‐Fang , Ho I‐Cheng , Miaw Shi‐Chuen 
Published: February 2010

AbstractThe function of transcription factors can be critically regulated by SUMOylation. c‐Maf, the cellular counterpart of v‐maf oncogene, is a potent transactivator of the IL‐4 gene in Th2 cells. We found in a yeast two‐hybrid screen that c‐Maf can interact with Ubc9 and PIAS1, two key enzymes of the SUMOylation pathway. In this study, we report that c‐Maf co‐localized with these two SUMO (small ubiquitin‐like modifier) ligases in the nucleus and that c‐Maf can be SUMOylated in vitro and also in primary Th2 cells. We also demonstrated that lysine‐33 is the dominant, if not the only, SUMO acceptor site of c‐Maf. SUMOylation of c‐Maf attenuated its transcriptional activity. Reciprocally, a SUMOylation resistant c‐Maf was more potent than WT‐c‐Maf in driving IL‐4 production in c‐Maf‐deficient Th2 cells. Furthermore, we showed that ablation of the SUMO site did not alter the subcellular localization or the stability of c‐Maf protein but instead enhanced its recruitment to the Il4‐promoter. We conclude that SUMOylation at lysine‐33 is a functionally critical post‐translational modification event of c‐Maf in Th cells.
The DC‐SIGN family member LSECtin is a novel ligand of CD44 on activated T cells
Authors: Tang Li , Yang Juntao , Tang Xiaoming , Ying Wantao , Qian Xiaohong , He Fuchu 
Published: February 2010

AbstractLSECtin, a novel member of the C‐type lectin DC‐SIGN family, not only acts as an attachment factor for pathogens, but also recognizes “endogenous” activated T cells. The endogenous ligands of LSECtin, however, have remained unclear. In this study, we identified CD44 on Jurkat T cells as a candidate ligand of LSECtin, and confirmed the specific interaction between LSECtin and CD44. Moreover, we showed that LSECtin selectively bound CD44s, CD44v4 and CD44v8‐10 by screening a series of typical CD44 isoforms. By deletion of the carbohydrate‐recognition domain region and mutation of crucial amino acids involved in carbohydrate‐recognition of LSECtin and by inhibition of the N‐linked glycosylation of CD44, we further demonstrated that the interaction between CD44 and LSECtin is dependent on protein‐glycan recognition. Our findings indicate that CD44 is the first identified endogenous ligand of LSECtin, and similarly, that LSECtin is a novel ligand of CD44. These findings provide important new perspectives on the biology of both LSECtin and CD44 in the immune system.
DEAD/H BOX 3 (DDX3) helicase binds the RIG‐I adaptor IPS‐1 to up‐regulate IFN‐β‐inducing potential
Authors: Oshiumi Hiroyuki , Sakai Keisuke , Matsumoto Misako , Seya Tsukasa 
Published: February 2010

AbstractRetinoic acid‐inducible gene‐I (RIG‐I)‐like receptors (RLR) are members of the DEAD box helicases, and recognize viral RNA in the cytoplasm, leading to IFN‐β induction through the adaptor IFN‐β promoter stimulator‐1 (IPS‐1) (also known as Cardif, mitochondrial antiviral signaling protein or virus‐induced signaling adaptor). Since uninfected cells usually harbor a trace of RIG‐I, other RNA‐binding proteins may participate in assembling viral RNA into the IPS‐1 pathway during the initial response to infection. We searched for proteins coupling with human IPS‐1 by yeast two‐hybrid and identified another DEAD (Asp‐Glu‐Ala‐Asp) box helicase, DDX3 (DEAD/H BOX 3). DDX3 can bind viral RNA to join it in the IPS‐1 complex. Unlike RIG‐I, DDX3 was constitutively expressed in cells, and some fraction of DDX3 is colocalized with IPS‐1 around mitochondria. The 622‐662 a.a DDX3 C‐terminal region (DDX3‐C) directly bound to the IPS‐1 CARD‐like domain, and the whole DDX3 protein also associated with RLR. By reporter assay, DDX3 helped IPS‐1 up‐regulate IFN‐β promoter activation and knockdown of DDX3 by siRNA resulted in reduced IFN‐β induction. This activity was conserved on the DDX3‐C fragment. DDX3 only marginally enhanced IFN‐β promoter activation induced by transfected TANK‐binding kinase 1 (TBK1) or I‐kappa‐B kinase‐ε (IKKε). Forced expression of DDX3 augmented virus‐mediated IFN‐β induction and host cell protection against virus infection. Hence, DDX3 is an antiviral IPS‐1 enhancer.
Diisobutylaluminium Hydride (DIBAL‐H) Promoted Secondary Rim Regioselective Demethylations of Permethylated β‐Cyclodextrin: A Mechanistic Proposal
Authors: Xiao Sulong , Yang Ming , Sinaÿ Pierre , Blériot Yves , Sollogoub Matthieu , Zhang Yongmin 
Published: February 2010

AbstractDiisobutylaluminium hydride (DIBAL‐H) promotes secondary rim regioselective bis‐de‐O‐methylation at the 2A‐ and 3B‐positions of permethylated β‐cyclodextrin. This result contrasts with the selective bis‐de‐O‐benzylation of perbenzylated cyclodextrins in which regioselective deprotection occurs at the primary rim. To gain an insight into the mechanism of this remarkable contrasting behavior, the two corresponding permethylated cyclodextrins with an alcohol function at either the 2‐ or 3‐position were synthesized. The cyclodextrin with the alcohol at the 3‐position reacts withDIBAL‐H to afford the 2A,3B‐diol whereas the cyclodextrin with the alcohol at the 2‐position is unreactive. This observation allows us to propose a mechanism for the demethylation reaction that involves for each demethylation step two molecules of aluminium reagent, in accord with the previous hypothesis on the debenzylation reaction. The second demethylation step appears to be faster than the first, the coordination of aluminium now being an intramolecular process.
Chiral Isocyanoazides: Efficient Bifunctional Reagents for Bioconjugation
Authors: Nenajdenko Valentine G. , Gulevich Anton V. , Sokolova Nadezhda V. , Mironov Andrey V. , Balenkova Elizabeth S. 
Published: February 2010

AbstractChiral scaffolds combining isocyanide and azide groups can be effectively synthesized to permit the efficient construction of both amino (hydroxy) acids and triazole derivatives, which enables the preparation of hybrid peptide molecules by Ugi/click or click/Ugi strategies.
Uncatalyzed Three‐Component Synthesis of α‐Hydrazido Phosphonates
Authors: Herrera Raquel P. , Roca‐López David , Navarro‐Moros Gloria 
Published: February 2010

AbstractVarious α‐hydrazido phosphonates have been easily prepared on the basis of the nucleophilic addition of diphenyl phosphite to reactive, preformed N‐acylhydrazones and a three‐component (aldehydes, N‐benzoylhydrazide and diphenyl phosphite) coupling reaction through a non‐catalyzed process. An unprecedented and promising enantioselective example of this reaction is also reported.