bluuebunny/crossref_metadata_2025_split huggingface dataset:
Developing a PmSLP3-based vaccine formulation that provides robust long-lasting protection against hemorrhagic septicemia–causing serogroup B and E strains of Pasteurella multocida in cattle
Authors: Fegan Jamie E. , Waeckerlin Regula C. , Tesfaw Liyuwork , Islam Epshita A. , Deresse Getaw , Dufera Dawit , Assefa Eyob , Woldemedhin Wubet , Legesse Abinet , Akalu Mirtneh , Bayissa Berecha , Nguyen Quynh Huong , Ng Dixon , Ahn Sang Kyun , Schryvers Anthony B. , Tefera Takele A. , Moraes Trevor F. , Gray-Owen Scott D. 
Published: May 2024

BackgroundPasteurella multocida is a bacterial pathogen that causes a variety of infections across diverse animal species, with one of the most devastating associated diseases being hemorrhagic septicemia. Outbreaks of hemorrhagic septicemia in cattle and buffaloes are marked by rapid progression and high mortality. These infections have particularly harmful socio-economic impacts on small holder farmers in Africa and Asia who are heavily reliant on a small number of animals kept as a means of subsistence for milk and draft power purposes. A novel vaccine target, PmSLP-3, has been identified on the surface of hemorrhagic septicemia–associated strains of P. multocida and was previously shown to elicit robust protection in cattle against lethal challenge with a serogroup B strain.MethodsHere, we further investigate the protective efficacy of this surface lipoprotein, including evaluating the immunogenicity and protection upon formulation with a variety of adjuvants in both mice and cattle.ResultsPmSLP-3 formulated with Montanide ISA 61 elicited the highest level of serum and mucosal IgG, elicited long-lasting serum antibodies, and was fully protective against serogroup B challenge. Studies were then performed to identify the minimum number of doses required and the needed protein quantity to maintain protection. Duration studies were performed in cattle, demonstrating sustained serum IgG titres for 3 years after two doses of vaccine and full protection against lethal serogroup B challenge at 7 months after a single vaccine dose. Finally, a serogroup E challenge study was performed, demonstrating that PmSLP-3 vaccine can provide protection against challenge by the two serogroups responsible for hemorrhagic septicemia.ConclusionTogether, these data indicate that PmSLP-3 formulated with Montanide ISA 61 is an immunogenic and protective vaccine against hemorrhagic septicemia-causing P. multocida strains in cattle.
Genetic associations in ankylosing spondylitis: circulating proteins as drug targets and biomarkers
Authors: Zhang Ye , Liu Wei , Lai Junda , Zeng Huiqiong 
Published: May 2024

BackgroundAnkylosing spondylitis (AS) is a complex condition with a significant genetic component. This study explored circulating proteins as potential genetic drug targets or biomarkers to prevent AS, addressing the need for innovative and safe treatments.MethodsWe analyzed extensive data from protein quantitative trait loci (pQTLs) with up to 1,949 instrumental variables (IVs) and selected the top single-nucleotide polymorphism (SNP) associated with AS risk. Utilizing a two-sample Mendelian randomization (MR) approach, we assessed the causal relationships between identified proteins and AS risk. Colocalization analysis, functional enrichment, and construction of protein-protein interaction networks further supported these findings. We utilized phenome-wide MR (phenMR) analysis for broader validation and repurposing of drugs targeting these proteins. The Drug-Gene Interaction database (DGIdb) was employed to corroborate drug associations with potential therapeutic targets. Additionally, molecular docking (MD) techniques were applied to evaluate the interaction between target protein and four potential AS drugs identified from the DGIdb.ResultsOur analysis identified 1,654 plasma proteins linked to AS, with 868 up-regulated and 786 down-regulated. 18 proteins (AGER, AIF1, ATF6B, C4A, CFB, CLIC1, COL11A2, ERAP1, HLA-DQA2, HSPA1L, IL23R, LILRB3, MAPK14, MICA, MICB, MPIG6B, TNXB, and VARS1) that show promise as therapeutic targets for AS or biomarkers, especially MAPK14, supported by evidence of colocalization. PhenMR analysis linked these proteins to AS and other diseases, while DGIdb analysis identified potential drugs related to MAPK14. MD analysis indicated strong binding affinities between MAPK14 and four potential AS drugs, suggesting effective target-drug interactions.ConclusionThis study underscores the utility of MR analysis in AS research for identifying biomarkers and therapeutic drug targets. The involvement of Th17 cell differentiation-related proteins in AS pathogenesis is particularly notable. Clinical validation and further investigation are essential for future applications.
A Mendelian randomization study on the causal effects of cigarette smoking on liver fibrosis and cirrhosis
Authors: Guo Liwei , An Yong , Huang Xu , Liu Wenhua , Chen Fangfang , Fan Yuchen , Gao Shuai , Han Liyan , Wang Kai 
Published: May 2024

BackgroundLiver fibrosis significantly impacts public health globally. Untreated liver fibrosis eventually results in cirrhosis. Cigarette smoking is the main etiologic factor for various diseases. However, the causal effects of cigarette smoking on liver fibrosis and cirrhosis have yet to be fully elucidated.MethodsIn this study, Mendelian randomization (MR) analysis was performed to assess the association between cigarette smoking, liver fibrosis, and cirrhosis. Single-nucleotide polymorphisms (SNPs) were selected as instrumental variables from a genome-wide association study (GWAS) of European ancestry. Patients were divided into six exposure categories as follows: “ever smoked,” “pack years of smoking,” “age of smoking initiation,” “smoking status: never,” “smoking status: current,” and “smoking status: previous.” The outcomes of this study included liver fibrosis and cirrhosis. MR-Egger, weighted median, inverse variance weighted, simple mode, and weighted mode were selected as the analysis methods. Cochran’s Q and the MR-PRESSO tests were conducted to measure heterogeneity. The MR-Egger method was performed to evaluate horizontal pleiotropy, while the “leave-one-out” analysis was performed for sensitivity testing.ResultsThe results of this study showed that having a smoking history increases the risk of liver fibrosis and cirrhosis [“ever smoked”: odds ratio (OR) = 5.704, 95% CI: 1.166–27.910, p = 0.032; “smoking status: previous”: OR = 99.783, 95% CI: 2.969–3.353e+03, p = 0.010]. A negative correlation was observed between patients who never smoked and liver fibrosis and cirrhosis (“smoking status: never”: OR = 0.171, 95% CI: 0.041–0.719, p = 0.016). However, there were no significant associations between “smoking status: current,” “pack years of smoking,” and “age of smoking initiation” and liver fibrosis and cirrhosis. Cigarette smoking did not have a significant horizontal pleiotropic effect on liver fibrosis and cirrhosis. The “Leave-one-out” sensitivity analysis indicated that the results were stable.ConclusionThe study confirmed the causal effects of cigarette smoking on liver fibrosis and cirrhosis.
Spatiotemporal consistency analysis of cerebral small vessel disease: an rs-fMRI study
Authors: Yang Jie , Xiao Rui , Liu Yujian , He Chaoliang , Han Limei , Xu Xiaoya , Chen Meining , Zhong Jianquan 
Published: May 2024

IntroductionCerebral small vessel disease (SVD) affects older adults, but traditional approaches have limited the understanding of the neural mechanisms of SVD. This study aimed to explore the effects of SVD on brain regions and its association with cognitive decline using the four-dimensional (spatiotemporal) consistency of local neural activity (FOCA) method.MethodsMagnetic resonance imaging data from 42 patients with SVD and 38 healthy controls (HCs) were analyzed using the FOCA values. A two-sample t test was performed to compare the differences in FOCA values in the brain between the HCs and SVD groups. Pearson correlation analysis was conducted to analyze the association of various brain regions with SVD scores.ResultsThe results revealed that the FOCA values in the right frontal_inf_oper, right temporal_pole_sup, and default mode network decreased, whereas those in the temporal_inf, hippocampus, basal ganglia, and cerebellum increased, in patients with SVD. Most of these varying brain regions were negatively correlated with SVD scores.DiscussionThis study suggested that the FOCA approach might have the potential to provide useful insights into the understanding of the neurophysiologic mechanisms of patients with SVD.
Increased usage of doxycycline for young children with Lyme disease
Authors: Thompson Amy D. , Neville Desiree N. , Chapman Laura L. , Balamuth Fran , Ladell Meagan M. , Kharbanda Anupam B. , Aresco Rachael , Nigrovic Lise E. 
Published: May 2024

BackgroundThe 2018 Infectious Disease Committee of the American Academy of Pediatrics stated that up to 3 weeks or less of doxycycline is safe in children of all ages. Our goal was to examine trends in doxycycline treatment for children with Lyme disease.MethodsWe assembled a prospective cohort of children aged 1 to 21 years with Lyme disease who presented to one of eight participating Pedi Lyme Net centers between 2015 and 2023. We defined a Lyme disease case with an erythema migrans (EM) lesion or positive two-tier Lyme disease serology categorized by stage: early-localized (single EM lesion), early-disseminated (multiple EM lesions, cranial neuropathy, meningitis, and carditis), and late (arthritis). We compared doxycycline treatment by age and disease stage and used logistic regression to examine treatment trends.ResultsOf the 1,154 children with Lyme disease, 94 (8.1%) had early-localized, 449 (38.9%) had early-disseminated, and 611 (53.0%) had late disease. Doxycycline treatment was more common for older children (83.3% ≥ 8 years vs. 47.1% &lt; 8 years; p &lt; 0.001) and with early-disseminated disease (77.2% early-disseminated vs. 52.1% early-localized or 62.1% late; p &lt; 0.001). For children under 8 years, doxycycline use increased over the study period (6.9% 2015 to 67.9% 2023; odds ratio by year, 1.45; 95% confidence interval, 1.34–1.58).ConclusionYoung children with Lyme disease are frequently treated with doxycycline. Prospective studies are needed to confirm the safety and efficacy of doxycycline in children younger than 8 years, especially for those receiving courses longer than 3 weeks.
Acceptance and rejection of “morally challenging” behaviour in online sperm donation communities: narrative interviews with recipients and donors
Authors: Forshall Georgina C. , Jones Georgina L. , Turner-Moore Rhys 
Published: May 2024

IntroductionOnline sperm donation allows those hoping to conceive a baby (“recipients”) to meet prospective sperm donors online, via “connection” websites or social media. These sites offer some advantages to clinical donation (including lower costs and greater choice over donation arrangements) but previous research has suggested that these sites may also pose risks and challenges to those who use them. Therefore, the aim of this exploratory research was to better understand online sperm donation communities and the experiences of both recipients and donors, particularly with respect to situations that could be “morally challenging” or involve harm.MethodsThree prolific donors and five recipients were interviewed using an in-depth narrative approach. Carol Gilligan's Listening Guide was employed to analyse the data.ResultsThe findings demonstrated that the donors sought to find ways to maintain autonomy in their donating practices and were concerned about the character and parenting abilities of recipients, as well as the potential for recipients to make unwarranted complaints. The recipients were concerned about their safety and finding a donor they could trust, discussing issues relating to donor “dishonesty”, online abuse, and a lack of support from connection sites and related authorities. Both donors and recipients identified “morally challenging” behaviour relating to donor anonymity (donor use of fake online profiles or aliases) and the sexual motivations or (mis)conduct of some donors. The participants each discussed the ways in which they managed perceived risks.DiscussionThe degree to which the participants voiced their acceptance or rejection of challenging behaviour in online sperm donation communities varied across and within participants, highlighting the complexity of the way in which people interact in this environment. Further research is required to understand how this form of sperm donation can be as safe and supportive as possible, while also respecting the importance to donors and recipients of autonomy and choice when making donation arrangements.
Gender/Sex Disparities in the COVID-19 Cascade From Testing to Mortality: An Intersectional Analysis of Swiss Surveillance Data
Authors: Auderset Diane , Amiguet Michaël , Clair Carole , Riou Julien , Pittet Valérie , Schwarz Joelle , Mueller Yolanda 
Published: May 2024

Objectives:This study investigates gender and sex disparities in COVID-19 epidemiology in the Canton of Vaud, Switzerland, focusing on the interplay with socioeconomic position (SEP) and age.Methods:We analyzed COVID-19 surveillance data from March 2020 to June 2021, using an intersectional approach. Negative binomial regression models assessed disparities between women and men, across SEP quintiles and age groups, in testing, positivity, hospitalizations, ICU admissions, and mortality (Incidence Rate Ratios [IRR], with 95% Confidence Intervals [CI]).Results:Women had higher testing and positivity rates than men, while men experienced more hospitalizations, ICU admissions, and deaths. The higher positivity in women under 50 was mitigated when accounting for their higher testing rates. Within SEP quintiles, gender/sex differences in testing and positivity were not significant. In the lowest quintile, women’s mortality risk was 68% lower (Q1: IRR 0.32, CI 0.20–0.52), with decreasing disparities with increasing SEP quintiles (Q5: IRR 0.66, CI 0.41–1.06).Conclusion:Our findings underscore the complex epidemiological patterns of COVID-19, shaped by the interactions of gender/sex, SEP, and age, highlighting the need for intersectional perspectives in both epidemiological research and public health strategy development.
Effect of Stationary Circuit and Moving Circuit Trainings on Selected Physical Fitness Components among University Men Students
Authors: Karthikeyan P 
Published: May 2024

The purpose of the study was to find out the effects of stationary circuit training [SCT] and Moving circuit training [MCT] on selected physical fitness components namely muscular endurance [ME] and cardio respiratory endurance [CRE] among university men students. To achieve this purpose of the study, forty-five men students studying men students studying Department of Physical Education, Annamalai University, Chidambaram, Tamil Nadu, India were selected as subjects at random. Their age ranged between 18 to 24 years. The selected subjects were divided into three equal groups of fifteen each namely stationary circuit training group [SCTG], Moving circuit training group [MCTG] and control group [CG]. The experimental group I underwent stationary circuit training [SCT] and group II underwent Moving circuit training [MCT] for three days per week for twelve weeks whereas the control group maintained their daily routine activities and no special training was given to them. The following physical fitness components, namely muscular endurance [ME] and cardio respiratory endurance [CRE] were selected as criterion variables. Thesubjects of the three groups were tested on selected physical fitness components namely muscular endurance and cardio respiratory endurance using standardized tests namely bend knee sit-ups and Cooper’s 12 min Run / Walk test at prior and immediately after the training period. The collecteddata were analyzed statistically through analysis of covariance (ANCOVA) to find out the significant difference, if any among the groups. Whenever the obtained “F” ratio was found to be significant,the Scheffe’s test was applied as post hoc test to find out the paired mean difference, if any. The .05 level of confidence was fixed to test the level of significance which was considered appropriate. The results of the study showed that there was a significant difference exist among SCTG, MCTG and CG on selected physical fitness components namely ME and CRE. And also, SCTG and MCTG showed significant improvement on ME and CRE when compared to control group.
PENGARUH PERSEPSI DUKUNGAN ORGANISASI TERHADAP KESEJAHTERAAN DI TEMPAT KERJA PADA KARYAWAN PT. X
Authors: Yusni Wisata  , Nita Rohayati  , Yulyanti Minarsih  
Published: May 2024

Penelitian ini bertujuan untuk mengetahui pengaruh persepsi dukungan organisasi terhadap kesejahteraan di tempat kerja pada karyawan PT X. Berbagai permasalahan yang ditemukan yaitu mengenai pemberian upah, kondisi kerja, penghargaan yang diberikan kepada karyawan, dan kurang nyaman di tempat kerja merupakan hal yang berkaitan dengan kesejahteraan karyawan di tempat kerja atau workplace wellbeing. Pengukuran dilakukan dengan mengadopsi alat ukur Survey of Perceived Organizational Support (SPOS) 8, sedangkan kesejahteraan di tempat kerja menggunakan alat ukur workplace wellbeing yang dikonstruksi. Subjek penelitian berjumlah 93 karyawan dan menggunakan teknik sampling total. Teknik analisis data menggunakan regresi linear sederhana dan diperoleh hasil persamaan regresi Y = 6,869 + 1,450X dengan nilai thitung sebesar 15,891> ttabel (1,986) dan signifikansi 0,000 < 0,05 maka H0 ditolak dan Ha diterima, artinya persepsi dukungan organisasi berpengaruh signifikan terhadap kesejahteraan di tempat kerja. Semakin tinggi tingkat persepsi dukungan organisasi, semakin tinggi kesejahteraan di tempat kerja pada karyawan. Sebaliknya, semakin rendah tingkat persepsi dukungan organisasi, maka semakin rendah pula kesejahteraan di tempat kerja yang dirasakan oleh karyawan. Persepsi dukungan organisasi mampu memberikan kontribusi sebesar 73,5 % terhadap kesejahteraan di tempat kerja, sedangkan sisanya sebesar 26,5 % merupakan faktor lain yang tidak diteliti dalam penelitian ini.
This research aims to determine the influence of perceived organizational support on workplace well-being among PT X employees. The various problems found, namely regarding wages, working conditions, awards given to employees, and lack of comfort in the workplace are matters related to employee welfare at work or workplace wellbeing. Measurements were carried out by adopting the Survey of Perceived Organizational Support (SPOS) 8 measuring instrument, while workplace well-being using a constructed workplace wellbeing measuring instrument. The research subjects were 93 employees and used a total sampling technique. The data analysis used a simple linear regression, showing that organizational support perception significantly influences workplace well-being, as the calculated t-value (15.891) exceeds the critical t-table value (1.986) with a significant level of 0.000 < 0.05. So, the null hypothesis (H0) was rejected, and the alternative hypothesis (Ha) was accepted. The higher the level of perceived organizational support, the higher the workplace well-being of employees. Conversely, the lower the level of perceived organizational support, the lower the well-being at work felt by employees. Perception of organizational support is able to contribute 73.5% to well-being in the workplace, while the remaining 26.5% is another factor not examined in this research.
OPTIMALISASI DIGITAL MARKETING MELALUI E-COMMERCE (PRESTASHOP) SEBAGAI STRATEGI PEMASARAN KAIN TENUN IKAT BANDAR KOTA KEDIRI
Authors: Zakaria Nizar , Saptaria Lina 
Published: May 2024

Pemanfaatan teknologi dalam kegiatan pemasaran menjadi kebutuhan penting bagi para pelaku usaha untuk mencapai efisiensi dan kinerja bisnis yang berkelanjutan. Strategi pemasaran maupun promosi produk kain tenun ikat Bandar Kota Kediri dinilai kurang sesuai dengan era digital,dan masyarakat pengetahuan. Permasalahan yang dihadapi oleh paguyuban diantaranya : banyak pelanggan yang belum mengetahui informasi tentang keunggulan produk, tidak mengenal merek produk yang ditawarkan, kurangnya minat beli konsumen terhadap produk, dan masih terbatasnya jangkauan pemasaran produk. Tujuan dari penelitian ini adalah merancang sistem e-commerce menggunakan Prestashop dan implementasinya untuk mengoptimalkan strategi pemasaran digital komunitas tenun ikat Bandar ikat Kota Kediri. Metode penelitian ini adalah deskriptif kualitatif dengan pendekatan studi kasus. Teknik pengumpulan data meliputi observasi dan wawancara. Analisis perancangan dan implementasi sistem menggunakan pendekatan sedikit demi sedikit (efisiensi, informasi, ekonomi, pengendalian, efisiensi, keamanan). Hasill penelitian ini berupa desain sistem e-commerce yang telah dimanfaatkan oleh paguyuban sebagai sarana promosi dan penjualan. Para informan yang terdiri dari 4 pakar (ahli) dan 2 anggota paguyuban maupun konsumen telah melakukan penilaian bahwa sistem e-commerce yang dirancang menggunakan PrestaShop efektif dan dapat memenuhi kebutuhan pengguna. Implikasi dari penelitian ini yaitu memberikan kontribusi dalam pengembangan sistem pemasaran digital yang efektif bagi pelaku UMKM tenun ikat Bandar Kota Kediri.
PENGARUH PENERAPAN BIMBINGAN BERBASIS KEARIFAN ETNIK UNTUK PENGEMBANGAN REGULASI DIRI ANAK USIA DINI DI RA AR-RIFA KECAMATAN SAMARANG
Authors: Haryawati Yayah , NOVIARTI RESTI , Rakhmat Cece , Supriatna Mamat 
Published: May 2024

Penelitian dilatarbelakangi fenomena di Raudhatul Athfal Ar-rifa berdasarkan survey awal, regulasi diri anak belum berkembang rata-ratanya sebesar 39,5 berada pada daerah interval 20 s.d 40 berada pada kategori rendah baik dalam Attention, cognitive flexibility, working memory maupun inhibitory control. Tujuan penelitian mendeskripsikan pengaruh penerapan bimbingan berbasis kearifan etnik untuk pengembangan regulasi diri. Menggunakan pendekatan kuantitatif desain survei, metode pengumpulan datanya melalui angket, teknik penyusunan instrumen serta validasi data kemudian dianalisis secara kuantitatif. Realitas pengaruh penerapan bimbingan berbasis kearifan etnik menunjukkan rata-rata 38,54 termasuk dalam kategori tinggi, kemudian realitas regulasi diri anak usia dini rata-rata 78,82 termasuk dalam kategori tinggi. Nilai korelasi sebesar 0,70 berkategori tinggi terletak pada rentang 0,60 s.d 0,80, maka diinterpretasikan penerapan bimbingan berbasis kearifan etnik di Raudhatul Athfal Ar-rifa melalui aspek permainan tradisional bakiak lari, indikatornya mengembangkan kemampuan berjalan cepat, mengkoordinasikan tubuh, melatih keseimbangan; kelenturan; kelincahan; dan kekompakan mempengaruhi regulasi diri anak usia dini; indikatornya fokus; berpikir dan bertindak sesuai dengan situasi dan dapat memecahkan masalah; memahami informasi yang diterima; mampu memahami pertanyaan; mengungkapkan ide dengan kalimat yang lebih kompleks; melawan godaan; dan menerapkan disiplin; berkembang dengan baik.

Kata Kunci : Kearifan etnik, Pengaruh, Regulasi diri