bluuebunny/crossref_metadata_2025_split huggingface dataset:
Interleukin‐6 (IL‐6) Does Not Change the Expression of Bcl‐2 Protein in the Prevention of Cisplatin‐Induced Apoptosis in Ovarian Cancer Cell Lines
Authors: Ishioka Shin‐ichi , van Haaften‐Day Caroline , Sagae Satoru , Kudo Ryuichi , Hacker Neville F. 
Published: May 2010

AbstractObjective: To study whether interleukin‐6 (IL‐6) changes the expression of the anti‐apoptic Bcl‐2 protein in the prevention of cis‐diaminedichloroplatinum (II) (CDDP)‐induced apoptosis of human ovarian cancer cells.Methods: Comparative studies were performed on 3 ovarian cancer cell lines after 48 hours of exposure to 0.5–50 ng/m/ IL‐6, 2 μg/m/ anti‐IL‐6 monoclonal antibody (anti‐IL‐6 mAb), 10 μm CDDP, 10 μm CDDP + 0.5–50 ng/m/ IL‐6, and 10 μm CDDP + 2 μg/m/ anti‐IL‐6 mAb. Apoptosis was measured morphologically and by a DNA fragmentation assay. Bcl‐2 protein levels were measured by an ELISA.Results: An increase in apoptosis was observed for each cell line after 48 hours of exposure to 10 μm CDDP. Although high doses of IL‐6 decreased the percentage of apoptotic cells, this cytokine did not change the expression of the Bcl‐2 protein.Conclusion: CDDP‐induced apoptosis was negatively controlled by IL‐6. However, the anti‐apoptic Bcl‐2 protein level was not changed by IL‐6 in the process of apoptosis in the ovarian cancer cell lines.
A Case Report: Rare Case of Primary Transitional Cell Carcinoma of the Fallopian Tube
Authors: Takeuchi Shigeto , Hirano Hiroshi , Ichio Takao , Taniguchi Haruki , Toyoda Nagayasu 
Published: May 2010

AbstractCarcinomas other than adenocarcinomas are extremely rare in the fallopian tube. A 42‐year‐old woman with watery, intermittent vaginal discharge was found to have a left adnexal tumor. This case was diagnosed as primary carcinoma of the fallopian tube, FIGO Stage la. She underwent a total abdominal hysterectomy, a bilateral salpingo‐oophorectomy, a pelvic and periaortic lymphoadenectomy, and an omentectomy, followed by cisplatin‐based chemotherapy. Four years after the initial diagnosis of the disease, she remains in a disease‐free state. Histologically, the tumor revealed a primary transitional cell carcinoma of the left fallopian tube. The findings on an immunohistochemical test for an epithelial membrane antigen, the CA125 antigen, were positive, whereas findings on a test for CEA were negative. We report a case of a malignant neoplasm of the fallopian tube with histological features of transitional cell carcinoma that arose from the tubal epithelium.
The Effect of Epidermal Growth Factor on the Preimplantation Development, Implantation and Its Receptor Expression in Mouse Embryos*
Authors: Kim Chung‐Hoon , Chae Hee‐Dong , Cheon Yong‐Pil , Kang Byung‐Moon , Chang Yoon‐Seok , Mok Jung‐Eun 
Published: May 2010

AbstractObjective: To investigate the influence of epidermal growth factor (EGF) on preimplantation development, implantation, and expression of epidermal growth factor receptor (EGFR) itself in mouse embryos.Materials and Method: Eight‐cell stage mouse embryos were cultured for 48 hours with EGF at concentrations of 0.1, 1.0, 10 and 100 ng/m/. Embryos not treated with EGF were served as control. The percentages of embryos which developed to the expanded, hatched blastocyst stage and in vitro implantation at 48 hours were determined. Reverse transcription‐polymerase chain reaction (RT‐PCR) has been used to examine the expression of EGFR in developed hatched blastocysts. Following reverse transcription, strategically designed nested primers, optimized for specificity, were used for amplification from the cDNA equivalent of a single embryo. The products were then verified by restriction enzyme digestion and sequence analysis. Results were analyzed with X2 test and Student's t‐test as apropriate, and statistical significance was defined as p < 0.05.Results: The percentages of fully expanded blastocysts at 48 hours in all the EGF treated group were not significantly different from the control. The percentages of hatched blastocysts were significantly higher in the EGF treatment group at 0.1 ng/m/ (90.5 ± 9.8%) compared to the control (82.1 ± 7.2%), 1.0 ng/m/ (82.2 ± 12.7%), and 100 ng/m/ (81.9 ± 11.8%) (p < 0.05, p < 0.05, p < 0.05, respectively). The percentages of hatched blastocysts were significantly higher in the EGF treatment group at 10 ng/m/ (89.4 ± 7.5%) compared to the control, and 100 ng/m/ (p < 0.05, p < 0.05, respectively). The percentages of attached blastocysts in vitro were significantly higher following incubation with EGF at concentrations of 0.1 ng/m/ (37.0 ± 17.0%), 1.0 ng/m/ (32.0 ± 14.3%), 10 ng/m/ (21.3 ± 7.2%) compared to the control (9.5 ± 7.7%) (p < 0.05, p < 0.05, p < 0.05, respectively). The attachment rates in 0.1 ng/m/ and 1.0 ng/m/ EGF treatment groups were also significantly higher than those in other EGF treatment groups. Embryo development and attachment were not significantly inhibited or enhanced in cultures supplemented with 100 ng/m/ EGF compared to the control. The mRNA concentration of EGFR in embryos treated with 0.1 ng/m/ of EGF was significantly higher than those of the control and other EGF treatment groups.Conclusion: EGF may have a stimulatory role in later stage embryonic development, implantation and expression of EGFR in hatched blastocyst itself at the specific concentration.
Assessment of Myometrial Invasion at the Invasion Site of an Endometrial Carcinoma by Ultrasonography Along with an Intrauterine Catheter
Authors: Perdomo Ilse A. , Miyagi Yasunari , Yamada Shinako , Kawanishi Kunihiro , Yamamoto Junko , Miyagi Yuji , Hongo Atsushi , Kodama Junichi , Yoshinouchi Mitsuo , Kobashi Yuji , Kudo Takafumi 
Published: May 2010

AbstractObjectives: To evaluate by transvaginal ultrasonography (TVU) the thickness of the intact myometrium at the presumed tumor‐origin site and to establish criteria for a half myometrial invasion.Methods: A total of 19 successive patients with endometrial cancer who were treated between January 1, 1997, and January 31, 1998, participated in this study. TVU mode B with and without the use of an intrauterine silicon catheter was performed.Results: Using a catheter, the origin site was correctly detected in 15 cases (79%). The best criterion for half myometrial invasion was a 6‐mm thickness of the intact myometrium at the origin site. The sensitivity/specificity/accuracy of TVU with the use of a catheter in cases with the correct estimated origin site, were 1.00/0.67/0.86 for myometrial invasion < 1/2, and 0.67/1.00/0.86 for myometrial invasion < 1/2.Conclusion: It is of value to use 6‐mm as the criterion for the thickness of the intact myometrium at the estimated tumor‐origin site in connection with TVU with the use of a catheter for preoperatively assessing half myometrial invasion.
A Randomized Controlled Trial Comparing Midwife‐Managed Care and Obstetrician‐Managed Care for Women Assessed to Be at Low Risk in the Initial Intrapartum Period
Authors: Law Yvonne Y. H. , Lam Kit‐Yee 
Published: May 2010

AbstractObjectives: To compare the efficacy of midwife‐managed care and obstetrician‐managed care for women assessed to be at low risk in the initial intrapartum period.Methods: 1,050 women assessed to be at low risk on admission to labour ward in the Prince of Wales Hospital participated in this study. By computer‐generated random allocation, 563 (54%) women were assigned to Group A (experimental) under midwifery care, and 487 (46%) women to Group B (control) under obstetrician care. The outcomes and complications between the 2 groups were compared. Data were analyzed by 2 times 2 contingency tables and Chi‐square.Results: 150 (26.6%) women in the experimental group were taken over by the obstetricians. 46 (30.7%) women were transferred to obstetrician‐management for the preference of epidural analgesia. The other reasons for taken over the remaining 104 (69.3%) women were fetal distress, poor progress of labour, complications in first or second stage of labour. The experimental group had less oxytocic augmentation (Chi‐square = 7.49, p = 0.006) and the insertion of intravenous infusion (Chi‐square = 5.34, p = 0.02). Both groups had similar outcomes on normal delivery, operative vaginal delivery, caesarean section and complications.Conclusions: Midwife‐managed care is as safe as obstetrician‐managed care for women who were assessed to be at low risk in the intrapartum period. Routine visit by obstetrician is not necessary and the midwives are able to detect complications in the course of labour and alert the obstetrician for taking the necessary action.
A Case of Simultaneous Presence of Primary Endometrial Carcinoma and Metastasis of a Breast Carcinoma to the Ovary after 5 Years of Tamoxifen Therapy
Authors: Ishioka Shin‐ichi , Sagae Satoru , Kobayashi Kanji , Sugimura Masaki , Nishioka Yoshihiro , Kudo Ryuichi 
Published: May 2010

AbstractWe report a case of a 43‐year‐old woman with the simultaneous presence of a primary uterine endometrial cancer and metastasis of breast cancer to the ovary after 5 years of tamoxifen therapy. Tamoxifen therapy lengthens recurrence‐free survival of the patient. However, the risk of endometrial cancer and the possibility of recurrence of breast cancer also must be considered.
Thermal Balloon Endometrial Ablator: A Preclinical Safety and Effectiveness Study
Authors: Banerjee Kaberi , Buckshee Kamal , Bhatla Neerja , Gupta Siddhartha Datta 
Published: May 2010

AbstractObjective: To evaluate the preclinical safety and efficacy of the thermal balloon endometrial ablator (TBEA).Methods: Informed consent was taken from all patients for TBEA and hysterectomy at same sitting. TBEA was performed in vivo just before hysterectomy on 6 patients' uteri to determine uterine rupture, perforation and thermal damage to surrounding viscera and peritoneum. Temperatures in the pouch of Douglas, uterovesical peritoneum and serosa of uterus were taken by a sterile laboratory thermometer to note for any rise in temperature before, during and after the procedure. The extirpated uteri were then examined grossly for the nature and extent of thermal damage which was visible as a zone of erythema in the endomyometrium. Five extirpated uteri, 3 from the above group and 2 on whom TBEA was performed 3 months ago were histopathologically examined to study the nature and extent of damage to endomyometrial cells. A 5 mm longitudinal strip of uterus along with both the cornual ends were sectioned in such a way that it was representative of the entire uterus.Results: There was no evidence of uterine perforation, rupture or any damage to the surrounding viscera and peritoneum. There was no rise in temperature in most sites while TBEA was performed. The only area that felt warm was the surface of the uterus and there too the measured rise was only 1°C. On gross examination the zone of erythema measured 5.4 mm (mean) (range 3–9 mm). In those uteri subjected to histopathology immediately after TBEA early evidence of thermal damage was visible as hemorrhage, congestion, edema, eosinophilic infiltration and necrosis in 2 out of 3 cases. Late changes of thermal damage after TBEA were seen as basal endometrium and areas of hyalinization. The changes in general were patchy and not uniform.Conclusions: TBEA is a safe device with no thermal damage to uterus and surrounding viscera. The mean zone of thermal damage in the endomyometrium is 5.4 mm. On histopathology, the early changes of thermal damage are hemorrhage, congestion, edema, eosinophilic infiltration and necrosis and the late changes are visible as areas of hyalinization and presence of basal endometrium.
Serum Vitamin A and E in Pregnant Women with Hemoglobinopathies
Authors: Phuapradit Winit , Panburana Panyu , Jaovisidha Adithep , Chanrachakul Boonsri , Bunyaratvej Ahnond , Puchaiwatananon Orawan 
Published: May 2010

AbstractObjective: To evaluate the relationship between the status of serum vitamin A, E and hemoglobinopathies among Thai pregnant women.Methods: This was a cross‐sectional study in which serum vitamin A and E were assessed in 323 pregnant women with normal hemoglobin and 73 with hemoglobinopathies (47 with hemoglobin E and 26 with thalassemia) during the first trimester.Results: There were no significant differences in the mean serum vitamin A, E concentrations and vitamin E/cholesterol ratio between pregnant women with normal hemoglobin and hemoglobinopathies, while confounding variables that might affect serum vitamin levels i.e. maternal age, gravida, BMI, gestational age, hematocrit, hemoglobin, mean corpuscular hemoglobin concentration and blood group were not different.Conclusion: The results of this study suggest that antenatal care in terms of micronutrients‐vitamin A, E in Thai pregnant women with hemoglobinopathies should not be different from normal pregnant women.
Early Discharge: Its Impact on Low‐Income Mothers and Newborns in the State of Hawaii
Authors: Taniguchi Hatsumi 
Published: May 2010

AbstractObjectives: The objectives of this survey were to investigate whether, in the state of Hawaii, early discharge had a negative effect on the health of new mothers and their infants, especially those at high sociodemographic risk, and whether early discharge was implemented in compliance with the early‐discharge guidelines of the American Academy of Pediatrics (AAP), and the American College of Obstetricians and Gynecologists (ACOG).Methods: This statewide survey was conducted from June 17 through July 12,1996. The data was collected and recorded by perinatal providers at 14 PSS (Perinatal Support Services) sites through interviews of mothers using questionnaires. This report is based on the 108 questionnaires that were returned from the PSS sites. A descriptive analysis was performed, using the statistical/epidemiologic program EPI INFO 6.Results: The survey reveals that the practice of early discharge had been implemented extensively but not in compliance with AAP/ACOG early discharge guidelines. The neonatal readmission rate for this survey group was 6.5% among low‐income mothers and infants. This was found to be among the highest rates reported in the United States and Canada; in the United States, reported neonatal readmission rates vary from 1 to 4%.Conclusions: Early discharge greatly contributes to negative outcomes among low‐income mothers and infants. This paper reports on the results of this survey in Hawaii and recommends a comprehensive approach for promoting the health and well‐being of low‐income mothers and infants.
A Clinicopathological Study of Postoperative Pulmonary Metastasis of Uterine Cervical Carcinomas
Authors: Shiromizu Kenji , Kasamatsu Takahiro , Takahashi Michiko , Kikuchi Akihiko , Yoshinari Tsutomu , Matsuzawa Masumi 
Published: May 2010

AbstractObjective: To investigate the clinicopathological backgrounds and prognostic factors of uterine cervical carcinomas metastatic to the lung.Methods: A total of 519 patients with invasive cervical carcinoma (Stage pTIb‐IIb) treated by abdominal radical hysterectomy at the Saitama Cancer Center from January 1, 1976 to December 31, 1989 were analyzed clinicopathologically.Results: The frequencies of pulmonary metastasis were 6.4% (24/377) and 11.3% (16/142) in patients with negative and positive pelvic lymph nodes, respectively. Among 24 negative lymph node patients, 15 had pulmonary metastasis only. The overall 5‐year survival rate of these 15 patients was 36% after relapse. Of the 15, the prognosis of 12 patients with 1–3 pulmonary metastases only was better, that is, 46% after surgical resection (mean size of resectable tumor = 2.8 cm) and / or chemotherapy. But the other patients died within 3.3 years after relapse.Conclusions: The occurrence of pulmonary metastasis only, its number (1–3) and size (mean size = 2.8 cm), and no lymph node metastasis are important prognostic factors. For these patients, active surgical resection of the pulmonary lesion(s) and further chemotherapy are recommended in order to improve their prognosis.
Comparison of the Anterior Colporrhaphy Procedure and the Marshall‐Marchetti‐Krantz Operation in the Treatment of Stress Urinary Incontinence among Women
Authors: Luna Maria Teresa C. , Hirakawa Toshio , Kamura Toshiharu , Yahata Hideaki , Ogawa Shinji , Sonoda Takanori , Nakano Hitoo 
Published: May 2010

AbstractObjective: To compare retrospectively the efficacy of the anterior colporrhaphy procedure (AC) and the Marshall‐Marchetti‐Krantz operation (MMK) in the treatment of stress urinary incontinence (SUI).Methods: A retrospective analysis through a review of the medical records of Japanese women with stress urinary incontinence who were surgically treated at Kyushu University Hospital from 1980 through 1996. A questionnaire regarding the current status of urinary incontinence was sent to all patients.Results: A total of 103 patients could be evaluated, 77 of whom had undergone an AC, and 26 of whom had undergone an MMK. Postoperative complications were more common in the AC group (p < 0.05). There were no significant differences between the 2 groups in terms of the duration of hospital stay or postoperative catheterization. The long‐term subjective cure rates of the ACs and the MMKs were 55% and 58%, respectively.Conclusions: The AC and the MMK were equally effective in treating stress urinary incontinence, and both showed decreased long‐term subjective cure rates. The recurrence rate did not differ between the AC and the MMK. The AC had more postoperative complications and shorter recurrence intervals.
Immunohistochemical Studies concerning Cathepsin D in Endometrial Carcinomas
Authors: Inoue Yoshiki , Abe Kunio , Obata Koshiro , Doh Kunihiko , Ohmura Gen , Hoshiai Hiroshi , Noda Kiichiro 
Published: May 2010

AbstractObjectives: To study the prognostic value of immunohistochemical detection of cathepsin D and laminin in endometrial carcinomas.Methods: Immunohistochemical staining of cathepsin D was performed on paraffin sections of 111 endometrial carcinomas, and laminin deposition was studied in 65 endometrial carcinomas.Results: Of 111 tissue specimens, 55 showed a positive reaction for cathepsin D. The incidence of cathepsin D‐positive staining increased with the extension of the primary tumor (p < 0.01). Patients with vessel invasion and pelvic lymph‐node metastasis had a higher incidence of cathepsin D‐positive staining than patients without these findings (p < 0.05). A favorable prognosis was obtained in a negative case of cathepsin D in comparison with a positive case (p < 0.01). Of 9 patients in whom laminin was detected in the cytoplasm of cancer cells, 5 showed poor prognoses and died from a primary disease.Conclusion: Cathepsin D and laminin status might represent possible prognostic factors.
Effects of Low Oxygen Condition on the Generation of Reactive Oxygen Species and the Development in Mouse Embryos Cultured in vitro*
Authors: Kwon Hyuck‐Chan , Yang Hyun‐Won , Hwang Kyung‐Joo , Yoo Jung‐Hyun , Kim Myung‐Sin , Lee Chi‐Hyeong , Ryu Hee Sug , Oh Kie Suk 
Published: May 2010

AbstractObjective(s): To elucidate the relationship between intracellular H2O2 production and embryo development in different oxygen culture conditions.Methods: Pronuclear stage embryos were obtained from C57BL/CBA F1 and ICR mice. Measurement of H2O2 level was performed with 2′,7′‐dichlorodihydroflourescein diacetate and the number of blastomeres was counted after staining with 4′,6′‐diamidino‐2‐phenylindole.Results: Regardless of strains, H2O2 level reached a peak at the 2‐cell stage in 20% O2. But in embryos cultured in 5% O2 it was significantly lower at the 2‐cell and 4‐cell stages compared to those from 20% O2. The embryos cultured in 20% O2 showed developmental delay or block, but in 5% O2 these phenomena were overcome and the development was significantly increased with an infrequent fragmentation.Conclusions: Our data suggest that the 5% O2 decreases the relative concentration of H2O2 and results in improved embryo development in terms of quantity and quality without regard to type of strains.
Significance of Multi‐Drug‐Resistant Proteins in Predicting Chemotherapy Response and Prognosis in Epithelial Ovarian Cancer*
Authors: Yokoyama Yoshihito , Sato Shigemi , Fukushi Yoshiyuki , Sakamoto Tomomi , Futagami Masayuki , Saito Yoshiharu 
Published: May 2010

AbstractObjectives: To clarify the expression of multi‐drug‐resistant (MDR) markers, GST‐π, c‐Jun, P‐glycoprotein (Pgp), and MDR‐associated protein (MRP) in epithelial ovarian cancer, and to determine whether their expression is predicitve of chemotherapy response and patient prognosis.Methods: Specimens of 58 epithelial ovarian cancer cases obtained at initial surgery were studied immunohistochemically using antibodies.Results: Overall positive rates in the 58 specimens were 58.6% for GST‐π, 44.8% for c‐Jun, 27.6% for Pgp, and 22.4% for MRP. The 5‐year disease‐free survival rate was 26.0% for patients with MRP‐positive tumors and 75.2% for those with MRP‐negative tumors. The prognosis for those with MRP‐positive tumors was significantly poorer (p < 0.05). Patients with GST‐π‐positive tumors had a significantly worse prognosis than those with GST‐π‐negative tumors (51.9% vs 79.2%, p < 0.05). Multivariate analysis showed that residual tumors 2 cm or larger and MRP expression were independent prognostic factors for chemotherapy resistance. The relative risk of chemotherapy resistance in a patient with a residual tumor 2 cm or larger, positive MRP, and positive GST‐π was 10.6 times greater than the risk in a patient without these factors.Conclusion: MRP and GST‐π expression might be potential predictors of the response to standard chemotherapy in epithelial ovarian cancer. Their expression also might contribute to individualizing clinical trials of postoperative chemotherapy.
The Rapid Biophysical Profile for Assessment of Fetal Well‐being
Authors: Tongsong Theera , Piyamongkol Wirawit , Anantachote Athittaya , Pulphutapong Karune 
Published: May 2010

AbstractObjective: To determine the efficacy of the rapid biophysical profile (BPP), the combination of amniotic fluid index (AFI) and sound‐provoked fetal movement (SPFM) detected by ultrasound, in predicting intrapartum fetal distress in high‐risk pregnancies, compared with the nonstress test (NST).Study Design: The prospective study of diagnostic tests was conducted on a total of 1,069 high‐risk singleton pregnancies, undergoing antepartum assessment of both the standard NST and the new rapid BPP, including AFI and SPFM detected by ultrasound. Intrapartum continuous fetal heart rate (FHR) monitoring was performed in all of them. The diagnostic indices of the NST and the rapid BPP was calculated in term of predicting intrapartum fetal distress.Results: The rapid BPP was a reliable predictor of intrapartum fetal distress with higher sensitivity and specificity. Its accuracy was better than that of the NST. The incidence of fetal compromise among positive, equivocal, and negative tests of the rapid BPP are 78.57, 15.82 and 0.9%, respectively, whereas the incidence among nonreactive and reactive NST are 31.63 and 2.52%, respectively.Conclusions: The rapid BPP is an effective predictor of intrapartum fetal distress in high‐risk pregnancies. It may suffice as an inexpensive and less time‐consuming method of evaluating antepartum fetal well‐being.
An Earlier Menopause as Clinical Manifestation of Granulosa‐Cell Tumor: A Case Report
Authors: Hiroi Hisahiko , Momoeda Mikio , Yamauchi Naoko , Abe Yumiko , Yoshikawa Hiroyuki , Tsutsumi Osamu , Taketani Yuji 
Published: May 2010

AbstractWe present a case of a granulosa‐cell tumor, which can cause menopause at an earlier than normal age. The hormonal profiles were characterized by undetectable FSH levels associated with an estradiol level compatible with the level seen in perimenopausal women and by a significant increase in the inhibin level.