bluuebunny/crossref_metadata_2025_split huggingface dataset:
Investigation of diachronic dietary patterns on the islands of Ibiza and formentera, Spain: Evidence from carbon and nitrogen stable isotope ratio analysis
Authors: Fuller Benjamin T. , Márquez‐Grant Nicholas , Richards Michael P. 
Published: June 2010

AbstractTo examine how dietary patterns may have changed in the western Mediterranean through time, stable carbon and nitrogen isotope ratios were measured on extracted bone collagen from fauna (n = 75) and humans (n = 135) spanning four distinct chronological periods: Chalcolithic (c.2100–1600 BC), Punic (6th–2nd/1st century BC), Late Antiquity‐Early Byzantine (4th–7th century AD), and Islamic (c.10th–13th century AD) on the islands of Ibiza and Formentera, Spain. The Chalcolithic, Punic, and Late Antiquity‐Byzantine societies all showed evidence of a predominately C3 terrestrial‐based diet with a possible input of a small amount of marine and/or C4 dietary resources. In contrast, the Islamic population on Ibiza had a subsistence strategy that was reliant on a significant amount of C4 plants and/or animals fed a C4 diet, likely millet. These results indicate a fairly constant C3 terrestrial‐based diet on the islands of Ibiza and Formentera through time, with a shift to C4 dietary resources during the Islamic Period. Further research is needed from other Islamic populations in and around the Mediterranean to better understand this unique dietary adaptation. Am J Phys Anthropol 143:512–522, 2010. © 2010 Wiley‐Liss, Inc.
Attenuation of osteoarthritis progression by reduction of discoidin domain receptor 2 in mice
Authors: Xu Lin , Servais Jacqueline , Polur Ilona , Kim Doil , Lee Peter L. , Chung Kimberly , Li Yefu 
Published: June 2010

AbstractObjectiveTo investigate whether the reduction of discoidin domain receptor 2 (DDR‐2), a cell membrane tyrosine kinase receptor for native type II collagen, attenuates the progression of articular cartilage degeneration in mouse models of osteoarthritis (OA).MethodsDouble‐heterozygous (type XI collagen–deficient [Col11a1+/−] and Ddr2‐deficient [Ddr2+/−]) mutant mice were generated. Knee joints of Ddr2+/− mice were subjected to microsurgical destabilization of the medial meniscus. Conditions of the articular cartilage from the knee joints of the double‐heterozygous mutant and surgically treated mice were examined by histology, evaluated using a modified Mankin scoring system, and characterized by immunohistochemistry.ResultsThe rate of progressive degeneration in knee joints was dramatically reduced in the double‐heterozygous mutant mice compared with that in the type XI collagen–deficient mice. The progression in the double‐heterozygous mutant mice was delayed by ∼6 months. Following surgical destabilization of the medial meniscus, the progressive degeneration toward OA was dramatically delayed in the Ddr2+/− mice compared with that in their wild‐type littermates. The articular cartilage damage present in the knee joints of the mice was directly correlated with the expression profiles of DDR‐2 and matrix metalloproteinase 13.ConclusionReduction of DDR‐2 expression attenuates the articular cartilage degeneration of knee joints induced either by type XI collagen deficiency or by surgical destabilization of the medial meniscus.
Accuracy and completeness of publication and citation records in the Web of Science, PsycINFO, and Google Scholar: A case study for the computation of h indices in Psychology
Authors: García‐Pérez Miguel A. 
Published: June 2010

AbstractHirsch's h index is becoming the standard measure of an individual's research accomplishments. The aggregation of individuals' measures is also the basis for global measures at institutional or national levels. To investigate whether the h index can be reliably computed through alternative sources of citation records, the Web of Science (WoS), PsycINFO and Google Scholar (GS) were used to collect citation records for known publications of four Spanish psychologists. Compared with WoS, PsycINFO included a larger percentage of publication records, whereas GS outperformed WoS and PsycINFO in this respect. Compared with WoS, PsycINFO retrieved a larger number of citations in unique areas of psychology, but it retrieved a smaller number of citations in areas that are close to statistics or the neurosciences, whereas GS retrieved the largest numbers of citations in all cases. Incorrect citations were scarce in Wos (0.3%), more prevalent in PsycINFO (1.1%), and overwhelming in GS (16.5%). All platforms retrieved unique citations, the largest set coming from GS. WoS and PsycINFO cover distinct areas of psychology unevenly, thus applying different penalties on the h index of researches working in different fields. Obtaining fair and accurate h indices required the union of citations retrieved by all three platforms.
Comparison of pre‐ and post‐processors for ensemble streamflow prediction
Authors: Kang Tae‐Ho , Kim Young‐Oh , Hong Il‐Pyo 
Published: June 2010

AbstractThis study conducted a broad review of the pre‐ and post‐processor methods for ensemble streamflow prediction using a Korean case study. Categorical forecasts offered by the Korea Meteorogical Administration and deterministic forecasts of a regional climate model called Seoul National University Regional Climate Model(SNURCM) were selected as climate forecast information for the pre‐processors and incorporated into Ensemble Streamflow Prediction(ESP) runs with the TANK hydrologic model. The post‐processors were then used to minimize a possible error propagated through the streamflow generation. The application results show that use of the post‐processor more effectively reduced the uncertainty of the no‐processor ESP than use of the pre‐processor, especially in dry season. Copyright © 2010 Royal Meteorological Society
Binding of licarbazepine enantiomers to mouse and human plasma proteins
Authors: Fortuna Ana , Alves Gilberto , Falcão Amílcar , Soares‐da‐Silva Patrício 
Published: June 2010

AbstractRacemic licarbazepine (Lic) is the active metabolite of oxcarbazepine (OXC) and eslicarbazepine acetate (ESL), appearing in human plasma as S‐licarbazepine (S‐Lic) and R‐licarbazepine (R‐Lic). However, human metabolism of OXC and ESL to Lic differs in the S‐Lic/R‐Lic enantiomeric ratio observed in plasma. S‐Lic appears in higher proportion after ESL administration than after OXC (95% versus 80%). Enantioselective pharmacokinetics of Lic enantiomers have been found in mice after their separate administration and in humans following OXC treatment. Since protein binding of drugs may be enantioselective and a determining factor of pharmacokinetics, the binding of S‐Lic and R‐Lic to mouse and human total plasma proteins and, specifically, to human serum albumin (HSA) and α1‐acid glycoprotein (AGP) were herein investigated for the first time. Free and bound fractions of S‐Lic and R‐Lic were separated by ultrafiltration after previous in vitro incubation of spiked plasma samples and protein solutions with each enantiomer at 10, 25 and 50 µg/ml. The results revealed that the extent of binding of Lic enantiomers to total plasma proteins was 30% and independent of the drug concentration and species considered. The data also suggest that the binding of Lic enantiomers to HSA is greater than that to AGP. Moreover, absence of enantioselectivity in the binding of Lic enantiomers to mouse and human plasma proteins and to HSA and AGP is evident. In conclusion, these findings suggest that the enantioselectivity observed in vivo in the biodisposition of S‐Lic and R‐Lic is not dependent on their affinity to plasma proteins. Copyright © 2010 John Wiley & Sons, Ltd.
Goldenhar syndrome associated with prenatal maternal Fluoxetine ingestion: Cause or coincidence?
Authors: Farra Chantal , Yunis Khalid , Mikati Mohammad , Yazbeck Nadine , Majdalani Marianne , Awwad Johnny 
Published: June 2010

AbstractGoldenhar syndrome, also known as oculo‐auriculo‐vertebral spectrum, is a complex, heterogeneous condition characterized by abnormal prenatal development of facial structures. We present the occurrence of Goldenhar syndrome in an infant born to a woman with a history of prenatal Fluoxetine ingestion throughout her pregnancy. Because this is the first reported case associating maternal Fluoxetine intake with fetal craniofacial malformations, a potential mechanism of injury is discussed. The propositus, a male born from nonconsanguinous parents, had facial asymmetry with right microtia and mandibular hypoplasia; he also had bilateral hypoplastic macula, scoliotic deformity of the thoracic spine, and ventricular septal defect. The mother was under treatment with Fluoxetine 20 mg/day prior to conception and maintained the same dosage throughout her pregnancy. The drug is a selective serotonin re‐uptake inhibitor, the most widely prescribed for the treatment of depression. The occurrence of developmental aberrations may be caused by a profound serotonin receptor suppressive state in utero leading to aberrant clinical manifestations of the first and second branchial arches. Despite the very many limitations of case reporting of teratogenic events, it remains an important source of information on which more advanced research is based. Birth Defects Research (Part A) 2010. © 2010 Wiley‐Liss, Inc.
Der Eurocode 2 für Deutschland – Erläuterungen und Hintergründe
Authors: Fingerloos Frank 
Published: June 2010

AbstractDer neue Eurocode 2: “Bemessung und Konstruktion von Stahlbeton‐ und Spannbetontragwerken – Teil 1‐1: Allgemeine Bemessungsregeln und Regeln für den Hochbau” (EC2‐1‐1) mit seinem Nationalen Anhang ist nach einer ausführlichen Erprobungsphase bereit zur bauaufsichtlichen Einführung in Deutschland. In mehreren Beiträgen sollen Erläuterungen und Hintergründe zu einigen Regelungen im Eurocode 2 und zu den Entscheidungen für Regeln im Nationalen Anhang vorgestellt werden, die entweder zu Änderungen gegenüber DIN 1045‐1 oder zu Abweichungen vom Eurocode 2 führten. Der Teil 1 gibt zunächst eine Übersicht über die maßgeblichen europäischen Normen und führt in den Nationalen Anhang ein.The Eurocode 2 for Germany – Explanations and Backgrounds. Part 1: Introduction in the National AnnexThe new Eurocode 2 “Design of concrete structures – Part 1‐1: General rules and rules for buildings” (EC2‐1‐1) and its National Annex has undergone a phase of detailed testing and is now ready for the implementation in Germany. In some articles should be given explanations and backgrounds of some rules in Eurocode 2 and of decisions for rules in the National Annex, which have been taken either to changes compared with DIN 1045‐1 or to differences to Eurocode 2. Article 1 gives an overview about the important European standards and introduces in the National Annex.
Modeling continuous auxiliary covariate data in generalized linear mixed models using the kernel smoother
Authors: Chen Jianwei , Lin Chii‐Dean 
Published: June 2010

AbstractAuxiliary covariate data are often collected in biomedical studies when the primary exposure variable is only assessed on a subset of the study subjects. In this study, we investigate a semiparametric‐estimated likelihood estimation for the generalized linear mixed models (GLMM) in the presence of a continuous auxiliary variable. We use a kernel smoother to handle continuous auxiliary data. The method can be used to deal with missing or mismeasured covariate data problems in a variety of applications when an auxiliary variable is available and cluster sizes are not too small. Simulation study results show that the proposed method performs better than that which ignores the random effects in GLMM and that which only uses data in the validation data set. We illustrate the proposed method with a real data set from a recent environmental epidemiology study on the maternal serum 1,1‐dichloro‐2,2‐bis(p‐chlorophenyl) ethylene level in relationship to preterm births.
Generation of high rapamycin producing strain via rational metabolic pathway‐based mutagenesis and further titer improvement with fed‐batch bioprocess optimization
Authors: Zhu Xiangcheng , Zhang Wei , Chen Xiyang , Wu Hu , Duan Yanwen , Xu Zhinan 
Published: June 2010

AbstractRapamycin is a triene macrolide antibiotic produced by Streptomyces hygroscopicus. Besides its wide application as an effective immunosuppressive agent, other important bioactivities have made rapamycin a potential drug lead for novel pharmaceutical development. However, the low titer of rapamycin in the original producer strain limits further industrialization efforts and restricts its use for other applications. Predicated on knowledge of the metabolic pathways related to rapamycin biosynthesis in S. hygroscopicus, we have rationally designed approaches to generate a rapamycin high producer strain of S. hygroscopicus HD‐04‐S. These have included alleviation of glucose repression, improved tolerance towards lysine and shikimic acid, and auxotrophy of tryptophan and phenylalanine through the application of stepwise UV mutagenesis. The resultant strain produced rapamycin at 450 mg/L in the shake flask scale. These fermentations were further scaled up in 120 and 20,000 L fermentors, respectively, at the pilot plant. Selected fermentation factors including agitation speed, pH, and on‐line supplementation were systematically evaluated. A fed‐batch strategy was established to maximize rapamycin production. With these efforts, an optimized fermentation process in the larger scale fermentor was developed. The final titer of rapamycin was 812 mg/L in the 120 L fermentor and 783 mg/L in the 20,000 L fermentor. This work highlights a high rapamycin producing strain derived by mutagenesis and subsequent screening, fermentation optimization of which has now made it feasible to produce rapamycin on an industrial scale by fermentation. The strategies developed here should also be applicable to titer improvement of other important microbial natural products on an industrial scale. Biotechnol. Bioeng. 2010;107: 506–515. © 2010 Wiley Periodicals, Inc.
Trichloroethene and cis‐1,2‐dichloroethene concentration‐dependent toxicity model simulates anaerobic dechlorination at high concentrations. II: Continuous flow and attached growth reactors
Authors: Sabalowsky Andrew R. , Semprini Lewis 
Published: June 2010

AbstractA model that was used to describe toxicity from high concentrations of chlorinated aliphatic hydrocarbons (CAHs) on reductively dechlorinating cultures in batch reactors (Sabalowsky and Semprini (in press)) was extended here to simulate observations in continuous flow suspended and attached growth reactors. The reductively dechlorinating anaerobic Evanite subculture (EV‐cDCE) was fed trichloroethene (TCE) and excess electron donor to accumulate cis‐1,2‐dichloroethene (cDCE) in a continuous flow stirred tank reactor (CFSTR); and an attached growth recirculating packed column (RPC). A concentration‐dependent toxicity model used to simulate the results of batch reactors in part I (Sabalowsky and Semprini (in press) Biotechnol Bioeng) also simulated well the observations for the CFSTR and RPC growth modes. The toxicity model incorporates cDCE and TCE toxicity coefficients that directly increase the cell decay coefficient in proportion with cDCE and TCE concentrations. Simulated estimates of the cDCE and TCE toxicity coefficients indicate reductively dechlorinating cells are most sensitive to high concentrations of cDCE and TCE in batch‐fed growth, followed by CFSTR, with attached growth being least sensitive. The greater toxicity of TCE than cDCE, and ratio of the modeled toxicity coefficients, agrees with previously proposed models relating toxicity to partitioning in the cell wall (KM/B), proportional to octanol‐water partitioning (KOW) coefficients. Biotechnol. Bioeng. 2010;107: 540–549. © 2010 Wiley Periodicals, Inc.
Microfluidic biolector—microfluidic bioprocess control in microtiter plates
Authors: Funke Matthias , Buchenauer Andreas , Schnakenberg Uwe , Mokwa Wilfried , Diederichs Sylvia , Mertens Alan , Müller Carsten , Kensy Frank , Büchs Jochen 
Published: June 2010

AbstractIn industrial‐scale biotechnological processes, the active control of the pH‐value combined with the controlled feeding of substrate solutions (fed‐batch) is the standard strategy to cultivate both prokaryotic and eukaryotic cells. On the contrary, for small‐scale cultivations, much simpler batch experiments with no process control are performed. This lack of process control often hinders researchers to scale‐up and scale‐down fermentation experiments, because the microbial metabolism and thereby the growth and production kinetics drastically changes depending on the cultivation strategy applied. While small‐scale batches are typically performed highly parallel and in high throughput, large‐scale cultivations demand sophisticated equipment for process control which is in most cases costly and difficult to handle. Currently, there is no technical system on the market that realizes simple process control in high throughput. The novel concept of a microfermentation system described in this work combines a fiber‐optic online‐monitoring device for microtiter plates (MTPs)—the BioLector technology—together with microfluidic control of cultivation processes in volumes below 1 mL. In the microfluidic chip, a micropump is integrated to realize distinct substrate flow rates during fed‐batch cultivation in microscale. Hence, a cultivation system with several distinct advantages could be established: (1) high information output on a microscale; (2) many experiments can be performed in parallel and be automated using MTPs; (3) this system is user‐friendly and can easily be transferred to a disposable single‐use system. This article elucidates this new concept and illustrates applications in fermentations of Escherichia coli under pH‐controlled and fed‐batch conditions in shaken MTPs. Biotechnol. Bioeng. 2010;107: 497–505. © 2010 Wiley Periodicals, Inc.
Long-term outcomes and quality of life after rectal carcinoma surgery
Authors: Hoerske C , Weber K , Goehl J , Hohenberger W , Merkel S 
Published: June 2010

Abstract

Background
A cohort study was undertaken to investigate the long-term oncological outcome, late adverse effects and quality of life (QOL) after treatment for rectal cancer.

Methods
This was an analysis of prospectively collected data from 268 consecutive patients with rectal carcinoma treated between 1995 and 1997 at the Department of Surgery, University Hospital Erlangen, Germany.

Results
Median follow-up was 8·8 years. The overall 10-year survival rate was 48·1 per cent. Of 219 patients who had a curative resection, 67 developed recurrent disease and 13 had second malignancies. Seventy patients had either a permanent stoma or a late adverse effect. Anorectal dysfunction and small bowel obstruction were significantly more common among patients who had multimodal treatment (P &lt; 0·001 and P = 0·049 respectively). Analysis of QOL in 97 long-term survivors showed that receiving chemoradiotherapy, a permanent stoma and lower-third rectal carcinoma were associated with significantly worse outcomes on several measures.

Conclusion
Late adverse effects and recurrences occurred in a significant number of patients during long-term follow-up. QOL varied according to tumour location and treatment type.
An approach to evaluate the reliability of hybridization‐based and sequencing‐based gene expression profiling technologies
Authors: Yang Dong‐Yan , Wang Xue‐Lin , Deng Ping‐Jian , Zhou Xiang‐Yang , Wu Xiao‐Jin , Wu Shui‐Qing , Yang Xiao‐Ke , Hou Hong‐Li , Yang Yong‐Cun , Zhang Hai‐Long , Liu Jin 
Published: June 2010

AbstractHybridization‐based and sequencing‐based technologies have found a widespread application in gene expression profiling analysis but much ambiguity exists regarding their reliability. This study developed a framework based on three parameters: detection ability, repeatability, and accuracy to evaluate the reliability of gene expression profiling technologies. The fraction of coverage of detected transcript category, the degree of variance for the number of differentially expressed genes (DEGs), the consistency of DEG category, and suspected false discovery rate (sFDR) were first introduced as statistical indices. In order to validate the availability of these indices, based on the same RNA extract, the analysis was performed by comparing gene expression differences between wild‐type and transgenic rice using deep sequencing and microarray. The results suggested that the parameters were available and showed advances in the determination of gene expression differences. Based on relative self‐comparison design, suspected false positive genes were easily identified from all DEGs detected, which was difficult for quantitative real‐time polymerase chain reaction (qRT‐PCR) validation when the count of DEGs was enormous. In addition, sFDRs had advantages in the accuracy evaluation for previous datasets. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010
On the choice of the optimal periodic operation for a continuous fermentation process
Authors: D'Avino G. , Crescitelli S. , Maffettone P. L. , Grosso M. 
Published: June 2010

AbstractIn this contribution we investigate the impact of the forcing waveform on the productivity of a continuous bioreactor governed by an unstructured, nonlinear kinetic model. The (periodic) forcing is applied on the substrate concentration in the feed. To this end, some alternative waveforms commonly encountered in practice are evaluated and their performance is compared. An analytical/numerical approach is used. The preliminary analytical step is based on the π‐criterion that gives useful information for small amplitudes. The extension to larger amplitudes, when significant improvements are expected, is then performed through a continuation‐optimization procedure. It is found that the choice of the specific waveform has an impact on the performance of the process and there is no unique best forcing for any process condition, but its choice depends on the operating parameters and the forcing amplitude and frequency values. Further, the influence of the waveform functions on the wash‐out conditions are extensively examined. The analysis shows that all the waveforms examined in this work may lead to significant enlargement of the nontrivial regime with respect to a steady state operation. In particular, square‐wave forcing leads in practice to the extinction of the wash‐out conditions for any feed substrate concentration and for a well defined choice of the forcing parameters. © 2010 American Institute of Chemical Engineers Biotechnol. Prog., 2010