bluuebunny/crossref_metadata_2025_split huggingface dataset:
Effectiveness, utilisation and cost associated with implantable loop recorders versus external monitors after ischaemic or cryptogenic stroke
Authors: Dhruva Sanket S , Murillo Jaime , Ameli Omid , Chaisson Christine E , Redberg Rita F , Cohen Ken 
Published: May 2024

ObjectiveImplantable loop recorders (ILRs) are increasingly used for long-term rhythm monitoring after ischaemic and cryptogenic stroke, with the goal of detecting atrial fibrillation (AF) and subsequent initiation of oral anticoagulation to reduce risk of adverse clinical outcomes. There is a need to determine the effectiveness of different rhythm monitoring strategies in this context.MethodsWe conducted a retrospective cohort analysis of individuals with commercial and Medicare Advantage insurance in Optum Labs Data Warehouse who had incident ischaemic or cryptogenic stroke and no prior cardiovascular implantable electronic device from 1 January 2016 to 30 June 2021. Patients were stratified by rhythm monitoring strategy: ILR, long-term continuous external cardiac monitor (>48 hours to 30 days) or Holter monitor (≤48 hours). The primary outcome was risk-adjusted all-cause mortality at 12 months. Secondary outcomes included new diagnosis of AF and oral anticoagulation, bleeding, and costs.ResultsAmong 48 901 patients with ischaemic or cryptogenic stroke, 9235 received an ILR, 29 103 long-term continuous external monitor and 10 563 Holter monitor only. Mean age was 69.9 (SD 11.9) years and 53.5% were female. During the 12-month follow-up period, patients who received ILRs compared with those who received long-term continuous external monitors had a higher odds of new diagnosis of AF and oral anticoagulant initiation (adjusted OR 2.27, 95% CI 2.09 to 2.48). Compared with patients who received long-term continuous external monitors, those who received ILRs had similar 12-month mortality (HR 1.00; 95% CI 0.89 to 1.12), with approximately $13 000 higher costs at baseline (including monitor cost) and $2500 higher costs during 12-month follow-up.ConclusionsIn this large real-world study of patients with ischaemic or cryptogenic stroke, ILR placement resulted in more diagnosis of AF and initiation of oral anticoagulation, but no difference in mortality compared with long-term continuous external monitors.
Single-Cell Analyses Reveal the Metabolic Heterogeneity and Plasticity of the Tumor Microenvironment during Head and Neck Squamous Cell Carcinoma Progression
Authors: Meng Xiaoyan , Zheng Yang , Zhang Lingfang , Liu Peipei , Liu Zhonglong , He Yue 
Published: May 2024

Abstract
Metabolic reprogramming is a hallmark of cancer. In addition to metabolic alterations in the tumor cells, multiple other metabolically active cell types in the tumor microenvironment (TME) contribute to the emergence of a tumor-specific metabolic milieu. Here, we defined the metabolic landscape of the TME during the progression of head and neck squamous cell carcinoma (HNSCC) by performing single-cell RNA sequencing on 26 human patient specimens, including normal tissue, precancerous lesions, early stage cancer, advanced-stage cancer, lymph node metastases, and recurrent tumors. The analysis revealed substantial heterogeneity at the transcriptional, developmental, metabolic, and functional levels in different cell types. SPP1+ macrophages were identified as a protumor and prometastatic macrophage subtype with high fructose and mannose metabolism, which was further substantiated by integrative analysis and validation experiments. An inhibitor of fructose metabolism reduced the proportion of SPP1+ macrophages, reshaped the immunosuppressive TME, and suppressed tumor growth. In conclusion, this work delineated the metabolic landscape of HNSCC at a single-cell resolution and identified fructose metabolism as a key metabolic feature of a protumor macrophage subpopulation.
Significance: Fructose and mannose metabolism is a metabolic feature of a protumor and prometastasis macrophage subtype and can be targeted to reprogram macrophages and the microenvironment of head and neck squamous cell carcinoma.
The Circadian Clock Component RORA Increases Immunosurveillance in Melanoma by Inhibiting PD-L1 Expression
Authors: Liu Dandan , Wei Benliang , Liang Long , Sheng Yue , Sun Shengjie , Sun Xing , Li Maohua , Li Haobo , Yang Chaoying , Peng Yuanliang , Xie Yifang , Wen Chengcai , Chen Lu , Liu Xionghao , Chen Xiang , Liu Hong , Liu Jing 
Published: May 2024

Abstract
Circadian clock perturbation frequently occurs in cancer and facilitates tumor progression by regulating malignant growth and shaping the immune microenvironment. Emerging evidence has indicated that clock genes are disrupted in melanoma and linked to immune escape. Herein, we found that the expression of retinoic acid receptor–related orphan receptor-α (RORA) is downregulated in melanoma patients and that patients with higher RORA expression have a better prognosis after immunotherapy. Additionally, RORA was significantly positively correlated with T-cell infiltration and recruitment. Overexpression or activation of RORA stimulated cytotoxic T-cell–mediated antitumor responses. RORA bound to the CD274 promoter and formed an inhibitory complex with HDAC3 to suppress PD-L1 expression. In contrast, the DEAD-box helicase family member DDX3X competed with HDAC3 for binding to RORA, and DDX3X overexpression promoted RORA release from the suppressive complex and thereby increased PD-L1 expression to generate an inhibitory immune environment. The combination of a RORA agonist with an anti-CTLA4 antibody synergistically increased T-cell antitumor immunity in vivo. A score based on the combined expression of HDAC3, DDX3X, and RORA correlated with immunotherapy response in melanoma patients. Together, this study elucidates a mechanism of clock component–regulated antitumor immunity, which will help inform the use of immunotherapy and lead to improved outcomes for melanoma patients receiving combined therapeutic treatments.
Significance: RORA forms a corepressor complex to inhibit PD-L1 expression and activate antitumor T-cell responses, indicating that RORA is a potential target and predictive biomarker to improve immunotherapy response in melanoma patients.
2024 AHA/ACC/AMSSM/HRS/PACES/SCMR Guideline for the Management of Hypertrophic Cardiomyopathy: A Report of the American Heart Association/American College of Cardiology Joint Committee on Clinical Practice Guidelines
Authors: Ommen Steve R. , Ho Carolyn Y. , Asif Irfan M. , Balaji Seshadri , Burke Michael A. , Day Sharlene M. , Dearani Joseph A. , Epps Kelly C. , Evanovich Lauren , Ferrari Victor A. , Joglar José A. , Khan Sadiya S. , Kim Jeffrey J. , Kittleson Michelle M. , Krittanawong Chayakrit , Martinez Matthew W. , Mital Seema , Naidu Srihari S. , Saberi Sara , Semsarian Christopher , Times Sabrina , Waldman Cynthia Burstein 
Published: May 2024

AIM:
The “2024 AHA/ACC/AMSSM/HRS/PACES/SCMR Guideline for the Management of Hypertrophic Cardiomyopathy” provides recommendations to guide clinicians in the management of patients with hypertrophic cardiomyopathy.

METHODS:
A comprehensive literature search was conducted from September 14, 2022, to November 22, 2022, encompassing studies, reviews, and other evidence on human subjects that were published in English from PubMed, EMBASE, the Cochrane Library, the Agency for Healthcare Research and Quality, and other selected databases relevant to this guideline. Additional relevant studies, published through May 23, 2023, during the guideline writing process, were also considered by the writing committee and added to the evidence tables, where appropriate.

STRUCTURE:
Hypertrophic cardiomyopathy remains a common genetic heart disease reported in populations globally. Recommendations from the “2020 AHA/ACC Guideline for the Diagnosis and Treatment of Patients With Hypertrophic Cardiomyopathy” have been updated with new evidence to guide clinicians.
Risk Factors for Intracerebral Hemorrhage: Genome-Wide Association Study and Mendelian Randomization Analyses
Authors: Larsson Susanna C. , Chen Jie , Gill Dipender , Burgess Stephen , Yuan Shuai 
Published: May 2024

BACKGROUND:
The genetic and nongenetic causes of intracerebral hemorrhage (ICH) remain obscure. The present study aimed to uncover the genetic and modifiable risk factors for ICH.

METHODS:
We meta-analyzed genome-wide association study data from 3 European biobanks, involving 7605 ICH cases and 711 818 noncases, to identify the genomic loci linked to ICH. To uncover the potential causal associations of cardiometabolic and lifestyle factors with ICH, we performed Mendelian randomization analyses using genetic instruments identified in previous genome-wide association studies of the exposures and ICH data from the present genome-wide association study meta-analysis. We performed multivariable Mendelian randomization analyses to examine the independent associations of the identified risk factors with ICH and evaluate potential mediating pathways.

RESULTS:

We identified 1 ICH risk locus, located at the
APOE
genomic region. The lead variant in this locus was rs429358 (chr19:45411941), which was associated with an odds ratio of ICH of 1.17 (95% CI, 1.11–1.20;
P
=6.01×10
−11
) per C allele. Genetically predicted higher levels of body mass index, visceral adiposity, diastolic blood pressure, systolic blood pressure, and lifetime smoking index, as well as genetic liability to type 2 diabetes, were associated with higher odds of ICH after multiple testing corrections. Additionally, a genetic increase in waist-to-hip ratio and liability to smoking initiation were consistently associated with ICH, albeit at the nominal significance level (
P
<0.05). Multivariable Mendelian randomization analysis showed that the association between body mass index and ICH was attenuated on adjustment for type 2 diabetes and further that type 2 diabetes may be a mediator of the body mass index-ICH relationship.

CONCLUSIONS:

Our findings indicate that the
APOE
locus contributes to ICH genetic susceptibility in European populations. Excess adiposity, elevated blood pressure, type 2 diabetes, and smoking were identified as the chief modifiable cardiometabolic and lifestyle factors for ICH.
Shifting between Pragmatism and Ideology: Communist Czechoslovakia and Haile Selassie's Ethiopia during the Cold War, 1955–1974
Authors: Záhořík Jan 
Published: May 2024

Abstract
Czechoslovakia was one of the most active members of the Soviet bloc in sub-Saharan Africa, with a policy oscillating between euphoric activism and pragmatism. Officials in Prague sought to establish good relations with many African countries, generally on a pragmatic economic basis, as in the case of Ethiopia. The Czechoslovak Communists gave high priority to Ethiopia because of the country's strategic position and its long-standing cooperation with Czechoslovakia. Although ideological factors played some role, economic pragmatism was the dominant feature of Czechoslovak policy in Africa in the 1950s and early 1960s. Not until the mid-1960s did the Soviet Union become the primary architect of the Soviet bloc's “Africa policy.” After the 1974 coup in Ethiopia, which saw the overthrow of the Haile Selassie regime, Czechoslovakia's relations with Ethiopia markedly improved for both ideological and pragmatic reasons.
Breaking the Strategic Glass: The Carter Administration and Oman, 1977–1980
Authors: White John Bernell 
Published: May 2024

Abstract
When Jimmy Carter became the U.S. president in January 1977, the United States and Oman did not have any formal security arrangements, apart from occasional landings of U.S. military aircraft on Oman's Masirah Island. Although Oman's leader, Sultan Qaboos bin Said, hoped to establish closer security ties with the United States, the Carter administration chose to continue President Richard Nixon's policy of relying on the “twin pillars” of Iran and Saudi Arabia to reinforce Western security in the Persian/Arabian Gulf region. Oman remained mostly a strategic afterthought until a series of crises unfolded in the greater Middle East: a Communist coup in Afghanistan in April 1978, the fall of the Shah of Iran in January 1979, and a war between Marxist South Yemen and North Yemen shortly thereafter. In November 1979, Iranian terrorists backed by the Islamic regime stormed the U.S. embassy in Tehran and seized dozens of hostages. This was followed a month later by the Soviet invasion of Afghanistan. With the fall of the Shah and the collapse of the “twin pillars” policy, the Carter administration began a sustained effort to create a Rapid Deployment Force for the Middle East and to gain access to Oman's strategic military facilities. After several months of arduous negotiations, Carter and his team were able to conclude a Facilities Access Agreement with Oman in June 1980, a transformative process that gave Washington the ability to insert U.S. military personnel into the Gulf and Indian Ocean on a contingency basis. The Carter administration's effort stands as a significant achievement, converting Oman into the most reliable U.S. ally in the Gulf region in the 1980s.
The Entropics of Discourse: the Nihilistic Teleology of Philipp Mainländer
Authors: Jensen Anthony K. 
Published: May 2024

Abstract
This article examines the teleological historical system of Philipp Mainländer, an under-researched 19th-century German philosopher in the Schopenhauerian tradition. From both theogonical and physical arguments, Mainländer endorses a theory of general entropy, whereby the sum of forces in the universe gradually expends itself to the point of annihilation. Consistent with this, Mainländer presents the course of history as the movement of individuals both politically and spiritually towards a paradoxical “willed will-lessness” in terms of a Socialist state and spiritual heaven.
Another new micro-endemic, limestone-dwelling leaf-toed gecko (Gekkonidae: Dixonius) from Phetchaburi Province, western Thailand
Authors: DONBUNDIT NATTASUDA , SUMONTHA MONTRI , SUTHANTHANGJAI MANEERAT , SUTHANTHANGJAI WINAI , PAUWELS OLIVIER S. G. 
Published: May 2024

We describe Dixonius chotjuckdikuli sp. nov. from Khao Ebid (Mount Ibit), Khao Yoi District, Phetchaburi Province, western Thailand. The new, limestone-dwelling species differs from all currently recognized species of Dixonius by the following combination of morphological characters and pattern: maximal known snout-vent length of 45.6 mm, 18 (rarely 16) longitudinal rows of dorsal tubercles; 31 to 34 paravertebral scales; 18 (rarely 16) longitudinal rows of ventral scales across the abdomen; 20 to 22 interciliary scales; 5 or 6 precloacal pores in males, no pores in females; a marked canthal stripe extending beyond the shoulder; and a blotched dorsal pattern in males, females and juveniles. This description brings the number of Dixonius species to 17, among which seven species are endemic to Thailand.
Need for consensus on primary end points and efficacy definitions in trials for adult acute lymphoblastic leukemia
Authors: Wieduwilt Matthew J. 
Published: May 2024

Abstract
The lack of consensus on acceptable primary end points and definitions of response and survival in phase 2/3 efficacy studies for adult acute lymphoblastic leukemia has led to widely different clinical trial designs. Inconsistency in primary end point selection and lack of consensus on response, survival end points, and adequate follow-up time lead to difficulty in interpreting completed studies and developing future trials. The lack of consensus also runs the risk of integrating ineffective or unacceptably toxic regimens into clinical practice and future trials. Increasingly, studies integrating highly active, targeted agents into chemotherapy use short-term end points of response, measurable residual disease–negative response, and early event-free survival without confidence that these end points will translate into improved late patient outcomes. This article highlights the current consequences and dilemmas caused by this lack of consensus. The hope is to stimulate discussion and ultimately consensus to improve the interpretation and application of clinical trial results.
Listeria brain abscess: a therapeutically challenging rare presentation of listeriosis
Authors: Bristowe Henrietta , Dissanayake Kishan , Chandra Julie , Arias Mauricio 
Published: May 2024

AbstractWe report a very rare case of Listeria multiple brain abscesses manifested as delirium, which represented diagnostic and therapeutic challenges overcome only by the close cooperation between Infectious Diseases and Neuroradiology, without which a satisfactory outcome would not be achieved.An elderly man presented with confusion and drowsiness with a background of type-II diabetes mellitus. Although computed tomography of the brain only showed frontal lobe oedema, contrast magnetic resonance (MR) imaging showed numerous irregular rim-enhancing lesions containing central diffusion restriction, suggesting multiple pyogenic cerebral abscesses of unclear aetiology. Thereafter, Listeria monocytogenes was isolated from blood cultures, suggesting this as the causative organism. Deemed unsuitable for neurosurgical drainage, the patient received medical management with a protracted course of antibiotics. This case was extremely challenging, due to 1) the impossibility of source control, 2) the small number of effective antibiotics available to treat this condition, and 3) the inevitable antibiotic side-effects, derived from long-term exposure. A successful outcome was only possible thanks to strict close multidisciplinary follow up, requiring frequent MR imaging and a judicious antibiotic choice, including monitoring of their side-effects. Due to the rarity of this condition, there is lack of guidance on its management, hence the importance of multidisciplinary involvement with very close imaging and antibiotic monitoring.
Association between NDUFS1 from urinary extracellular vesicles and decreased differential renal function in children with ureteropelvic junction obstruction
Authors: Bu Lingyun , Zhang Lingling , Wang Xiaoqing , Du Guoqiang , Wu Rongde , Liu Wei 
Published: May 2024

Abstract
Background
Ureteropelvic junction obstruction (UPJO) is the most common cause of pediatric congenital hydronephrosis, and continuous kidney function monitoring plays a role in guiding the treatment of UPJO. In this study, we aimed to explore the differentially expressed proteins (DEPs) in the urinary extracellular vesicles(uEVs) of children with UPJO and determine potential biomarkers of uEVs proteins that reflect kidney function changes.

Methods
Preoperative urine samples from 6 unilateral UPJO patients were collected and divided into two groups: differential renal function (DRF) ≥ 40% and DRF < 40%.We subsequently used data-independent acquisition (DIA) to identify and quantify uEVs proteins in urine, screened for DEPs between the two groups, and analyzed biofunctional enrichment information. The proteomic data were evaluated by Western blotting and enzyme-linked immunosorbent assay (ELISA) in a new UPJO testing cohort.

Results
After one-way ANOVA, a P adj value < 0.05 (P-value corrected by Benjamin–Hochberg) was taken, and the absolute value of the difference multiple was more than 1.5 as the screening basis for obtaining 334 DEPs. After analyzing the enrichment of the DEPs according to Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment combined with the protein–protein interaction (PPI) network results, we selected nicotinamide adenine dinucleotide-ubiquinone oxidoreductase core subunit S1 (NDUFS1) for further detection. The expression of NDUFS1 in uEVs was significantly lower in patients with DRF < 40% (1.182 ± 0.437 vs. 1.818 ± 0.489, P < 0.05), and the expression level of NDUFS1 was correlated with the DRF in the affected kidney (r = 0.78, P < 0.05). However, the NDUFS1 concentration in intravesical urine was not necessarily related to the change in DRF (r = 0.28, P = 0.24).

Conclusions
Reduced expression of NDUFS1 in uEVs might indicate the decline of DRF in children with UPJO.
Glycometabolism and lipid metabolism related genes predict the prognosis of endometrial carcinoma and their effects on tumor cells
Authors: Lin Xuefen , Zheng Jianfeng , Cai Xintong , Liu Li , Jiang Shan , Liu Qinying , Sun Yang 
Published: May 2024

Abstract
Background
Glycometabolism and lipid metabolism are critical in cancer metabolic reprogramming. The primary aim of this study was to develop a prognostic model incorporating glycometabolism and lipid metabolism-related genes (GLRGs) for accurate prognosis assessment in patients with endometrial carcinoma (EC).

Methods
Data on gene expression and clinical details were obtained from publicly accessible databases. GLRGs were obtained from the Genecards database. Through nonnegative matrix factorization (NMF) clustering, molecular groupings with various GLRG expression patterns were identified. LASSO Cox regression analysis was employed to create a prognostic model. Use rich algorithms such as GSEA, GSVA, xCELL ssGSEA, EPIC,CIBERSORT, MCPcounter, ESTIMATE, TIMER, TIDE, and Oncoppredict to analyze functional pathway characteristics of the forecast signal, immune status, anti-tumor therapy, etc. The expression was assessed using Western blot and quantitative real-time PCR techniques. A total of 113 algorithm combinations were combined to screen out the most significant GLRGs in the signature for in vitro experimental verification, such as colony formation, EdU cell proliferation, wound healing, apoptosis, and Transwell assays.

Results
A total of 714 GLRGs were found, and 227 of them were identified as prognostic-related genes. And ten GLRGs (AUP1, ESR1, ERLIN2, ASS1, OGDH, BCKDHB, SLC16A1, HK2, LPCAT1 and PGR-AS1) were identified to construct the prognostic model of patients with EC. Based on GLRGs, the risk model’s prognosis and independent prognostic value were established. The signature of GLRGs exhibited a robust correlation with the infiltration of immune cells and the sensitivity to drugs. In cytological experiments, we selected HK2 as candidate gene to verify its value in the occurrence and development of EC. Western blot and qRT-PCR revealed that HK2 was substantially expressed in EC cells. According to in vitro experiments, HK2 knockdown can increase EC cell apoptosis while suppressing EC cell migration, invasion, and proliferation.

Conclusion
The GLRGs signature constructed in this study demonstrated significant prognostic value for patients with endometrial carcinoma, thereby providing valuable guidance for treatment decisions.