bluuebunny/crossref_metadata_2025_split huggingface dataset:
Identification of novel target proteins in sebaceous gland carcinoma
Authors: Erovic Boban M. , Al Habeeb Ayman , Harris Luke , Goldstein David P. , Kim Dae , Ghazarian Danny , Irish Jonathan C. 
Published: June 2012

AbstractBackgroundThe aim of this study was to identify new target proteins in sebaceous gland carcinoma.MethodsA tissue microarray containing 115 core biopsies was constructed and stained for proteins involved in carcinogenesis, angiogenesis, inflammation, and cell‐to‐cell contact. Two investigators independently determined protein expression of all antibodies.ResultsVascular endothelial growth factor receptor 2 (VEGFR‐2), platelet‐derived growth factor receptor alpha and beta (PDGFR‐α/‐β), epidermal growth factor receptor (EGFR), cyclooxygenase 1 and 2 (Cox‐1/‐2), myeloid cell leukemia sequence 1 (Mcl‐1), matrix metalloproteinase 1 (MMP‐1), CD9, Bmi‐1, 14‐3‐3σ, glutathione S‐transferase pi (Gstπ), and members of the sonic hedgehog (SHH), AKT, and WNT pathways were significantly overexpressed in sebaceous gland carcinomas.ConclusionsWe have demonstrated for the first time that proteins related to angiogenesis, inflammation, and cell proliferation are overexpressed in sebaceous gland carcinomas. These proteins may hold promise as novel therapeutic targets for the treatment of sebaceous gland carcinoma. © 2012 Wiley Periodicals, Inc. Head Neck, 2013
Usefulness of cone‐beam CT for presurgical assessment of keratoma (cholesteatoma) of the maxillary sinus
Authors: Buric Nikola , Jovanovic Goran , Tijanic Milos 
Published: June 2012

AbstractBackgroundEvaluating the presurgical usefulness of cone‐beam CT (CBCT) in the diagnosis and subsequent surgery of keratoma (cholesteatoma) of the maxillary sinus.MethodsCBCT scans were obtained by using 3‐dimensional CT (3DCT) apparatus. The exposure factors had a field of view of 16 × 12 cm, 120 kVp, 7 mA, and an exposure time of 20 seconds. The acquired image data consisted of a 14‐bit sensor that delivers 16,384 shades of gray with a standard isotropic cubic voxel size of 0.3 mm.ResultsThe CBCT scans clearly demonstrated a 22‐ × 16.82‐ × 17.62‐mm expansile lesion in the left maxillary sinus area, with thin cortical plates, and a focal perforation of the medial cortical plate at the level of the apices.ConclusionAll CBCT visual data provide impressive, full 3D images of maxillary sinus keratoma, and help us to perform successful surgery. © 2012 Wiley Periodicals, Inc. Head Neck, 2013
Lyase activity of glycogen synthase: Is an elimination/addition mechanism a possible reaction pathway for retaining glycosyltransferases?
Authors: Díaz Adelaida , Díaz‐Lobo Mireia , Grados Enrique , Guinovart Joan J. , Fita Ignacio , Ferrer Joan C. 
Published: June 2012

AbstractDespite the biological relevance of glycosyltrasferases (GTs) and the many efforts devoted to this subject, the catalytic mechanism through which a subclass of this large family of enzymes, namely those that operate with net retention of the anomeric configuration, has not been fully established. Here, we show that in the absence of an acceptor, an archetypal retaining GT such as Pyrococcus abyssi glycogen synthase (PaGS) reacts with its glucosyl donor substrate, uridine 5'‐diphosphoglucose (UDP‐Glc), to produce the scission of the covalent bond between the terminal phosphate oxygen of UDP and the sugar ring. X‐ray diffraction analysis of the PaGS/UDP‐Glc complex shows no electronic density attributable to the UDP moiety, but establishes the presence in the active site of the enzyme of a glucose‐like derivative that lacks the exocyclic oxygen attached to the anomeric carbon. Chemical derivatization followed by gas chromatography/mass spectrometry of the isolated glucose‐like species allowed us to identify the molecule found in the catalytic site of PaGS as 1,5‐anhydro‐D‐arabino‐hex‐1‐enitol (AA) or its tautomeric form, 1,5‐anhydro‐D‐fructose. These findings are consistent with a stepwise SNi‐like mechanism as the modus operandi of retaining GTs, a mechanism that involves the discrete existence of an oxocarbenium intermediate. Even in the absence of a glucosyl acceptor, glycogen synthase (GS) promotes the formation of the cationic intermediate, which, by eliminating the proton of the adjacent C2 carbon atom, yields AA. Alternatively, these observations could be interpreted assuming that AA is a true intermediate in the reaction pathway of GS and that this enzyme operates through an elimination/addition mechanism. © 2012 IUBMB Life, 2012.
Modulation of temporomandibular joint nociception and inflammation in male rats after administering a physiological concentration of 17β‐oestradiol
Authors: Kramer P.R. , Bellinger L.L. 
Published: June 2012

AbstractBackgroundPrevious studies have shown 17β‐estradiol will reduce temporomandibular joint (TMJ) inflammation and hypersensitivity in female rats. Although male rats contain significant amounts of oestradiol, it was unknown whether a physiological concentration of 17β‐estradiol would attenuate male TMJ inflammation and nociception.MethodsIntact and castrated rats were given a physiological concentration of oestradiol to examine first, if oestradiol will affect male TMJ nociception/inflammation and, second, if administration of oestradiol would act synergistically with endogenous male hormones to attenuate TMJ nociception. The hormonally treated rats were given TMJ injections of complete Freund's adjuvant (CFA) and then nociception was measured using a validated method in which a lengthening in meal duration is directly correlated to the intensity of deep TMJ nociception. Inflammation was assayed by quantitating pro‐inflammatory gene expression.ResultsMeal duration was significantly lengthened after TMJ CFA injection and this lengthening was significantly attenuated in the castrated but not intact males after administering a physiological concentration of oestradiol. A physiological concentration of 17β‐estradiol also significantly increased IL‐6 expression in the inflamed TMJ of castrated males while 17β‐estradiol did not alter IL‐1β, CXCL2 and CCL20 expression. Castration increased pro‐inflammatory mediators IL‐6, IL‐1β and CXCL2 suggesting male sex hormones were anti‐inflammatory. Calcitonin gene‐related peptide in the trigeminal ganglia was unchanged.ConclusionsSimilar to females, male rats with TMJ inflammation showed a reduced nociceptive response after treatment with a physiological concentration of oestradiol suggesting the effects of oestradiol treatment were not constrained by organizational processes in the males.
KEEPING MEANS AND ENDS IN VIEW—LINKING PRACTICAL JUDGEMENT, ETHICS AND EMERGENCE
Authors: Mowles Chris 
Published: June 2012

AbstractIn this article I argue that the means and ends of development are constitutive of each other. If means appear to have become separated from the espoused ends of international nongovernment organisations (INGOs), then it may be because other ends, the corporate aspirations of INGOs often expressed in grand strategies and plans, have come to dominate. However, in recognising that in development management particular methods that privilege the abstract and instrumental have come to dominate, this does not close off all possibility of critical reflection, discussion and negotiation, through the exercise of practical judgement or phronesis. Wholesale schemes for doing good, no matter how idealised and abstract, must be functionalised in particular contexts with particular people and can still be negotiated, affording the potential for the emergence of greater mutual recognition between those engaged in development. This negotiation and exploration of difference is often uncomfortable as each of the parties, and their power relationships, is made more visible to the other, but, this article argues, it is a prerequisite for continuously appraising our ethical engagement with others and for keeping means and ends in view. Copyright © 2012 John Wiley & Sons, Ltd.
ENGAGEMENT, LEARNING AND EMANCIPATORY DEVELOPMENT MANAGEMENT: A COMMENTARY
Authors: Johnson Hazel , Pinder Richard , Wilson Gordon 
Published: June 2012

AbstractComplex and constantly emerging relationships between groups, organisations and movements aiming to promote and manage change bring into sharp focus the nature of means and ends and how they interact. Development managers are continually negotiating these relationships, where the tools (the means) and the espoused goals of development (the ends) are caught up in the process. We argue that although it is essential to recognise the role of power relations in development management, social difference and contestation experienced in the negotiation of changing relationships can create spaces and be important sources of engagement and learning for development managers and their organisations. A question is whether and how such learning can lead to more emancipatory means and ends of development management, rather than simply consolidating existing social relations. In this article, we provide a commentary on these issues through interviews with a small group of practitioners working mainly in non‐governmental organisations. Copyright © 2012 John Wiley & Sons, Ltd.
THE PEOPLE NEED TO MAKE THE TRAINS RUN ON TIME FOR EMPOWERMENT TO HAPPEN: MANAGERIALISM AND REVOLUTION IN NICARAGUA
Authors: Stookey Sarah 
Published: June 2012

AbstractThis article extends Gulrajani's proposal (2007) for a non‐managerialist alternative to the dichotomy posited by the radical and reformist critiques of development management. Drawing on extensive personal participation in development work during and after the Sandinista period, the author proposes that in Nicaragua a practice of development management emerged that defies the radical–reformist dichotomy. Unlike the alternative that Gulrajani proposed, it is argued that this practice had managerialist characteristics but that this reinforced rather than undermined progressive social change. The occurrence of this managerialism of the revolution, grounded in the Sandinista ethos of popular power and shaped by local history, conditions, and the extreme demands of national defence, suggests that development ends and managerialism are not necessarily antithetical. Copyright © 2012 John Wiley & Sons, Ltd.
Persistence of immunity to tick‐borne encephalitis after vaccination and natural infection
Authors: Baldovin Tatjana , Mel Rosanna , Bertoncello Chiara , Carpenè Graziella , Soppelsa Fabio , Giliberti Aurore , Baldo Vincenzo 
Published: June 2012

AbstractLong‐term persistence of immunity was assessed in 66 patients who had contracted tick‐borne encephalitis (TBE) and in 126 subjects who had completed primary TBE immunization using a conventional three‐dose schedule from 3 to 8 years earlier. Immunity was tested in the subjects stratified by age as follows: ≤40 years (N = 37); 41–60 years (N = 100); and over 60 years (N = 55). Antibody levels decreased significantly with increasing age in the vaccinated cohort by comparison with the individuals who had previously contracted TBE. Consistently higher geometric mean antibody levels were found in the patients infected naturally. When the vaccinated subjects were compared, subjects ≤40 years old had significantly higher antibody levels than either of the older groups. Analyzing immunity to TBE over time revealed a remarkable (50%) decline in seroprotection rates in the vaccinated group at 50 months of follow‐up, while stable, high levels persisted in all subjects after natural TBE infection. In the vaccinees over 60 years old, the TBE antibody levels reached 60% at 60 months, and 20% at 70 months of follow‐up; in contrast, in the 41–60‐year‐old group, the antibody levels remained high for 70 months, and then fell rapidly. For people aged <60 years old, booster doses are recommended every 5 years after the fourth dose of vaccine, which should be administered 3 years after primary immunization. In subjects aged 60 years or older, booster doses should be given every 3 years. J. Med. Virol. 84: 1274–1278, 2012. © 2012 Wiley Periodicals, Inc.
Genetic characterization of seoul hantavirus originated from norway rats (Rattus norvegicus) captured in Belgium
Authors: Plyusnina Angelina , Heyman Paul , Baert Kristof , Stuyck Jan , Cochez Christel , Plyusnin Alexander 
Published: June 2012

AbstractHantaviruses (genus Hantavirus, family Bunyaviridae) cause hemorrhagic fever with renal syndrome (HFRS) in Eurasia and hantavirus (cardio)pulmonary syndrome (HCPS) in the Americas. So far, in Europe, four pathogenic hantaviruses have been found, often in co‐circulation: Puumala virus (PUUV), Dobrava virus (DOBV), Saaremaa virus (SAAV), and Seoul virus (SEOV). Of those, only PUUV was found in Belgium. Recently, in our search for hantaviruses in the Flanders region of Belgium we collected genetic and serological evidence for the presence of SEOV virus in local brown rats. In this article, the results of (phylo)genetic analysis of wild‐type SEOV strain from the Flanders are presented. The analysis based on the complete S segment sequence and also partial M‐ and L‐segment sequences revealed that the Belgian SEOV strain was related most closely to strains from France, Indonesia, Japan, Singapore, Cambodia (those associated with the species Rattus norvegicus) and Vietnam. Such a clustering was in perfect agreement with the results of direct sequence comparison and suggested the same evolutionary history for all three genome segments of the Belgian SEOV strain (i.e., no reassortment of genome segments). So far, SEOV has been found in two European countries, France and Belgium, and there is every reason to believe that the area of the virus distribution in Europe is not restricted to those countries. J. Med. Virol. 84: 1298–1303, 2012. © 2012 Wiley Periodicals, Inc.
In vitro replication phenotype of a novel (−1G) hepatitis B virus variant associated with HIV co‐infection
Authors: Cabuang Liza M. , Shaw Tim , Littlejohn Margaret , Colledge Danni , Sozzi Vitini , Soppe Sally , Warner Nadia , Thompson Alex , Preiss Scott , Lam Natasha , Walsh Renae , Lewin Sharon R. , Thio Chloe L. , Matthews Gail , Locarnini Stephen A. , Revill Peter A. 
Published: June 2012

AbstractThe −1G mutant HBV is more prevalent in individuals co‐infected with HIV/HBV than in individuals infected with HBV alone and in some cases is the dominant virus in circulation. This mutant is created by the deletion of a dGMP (−1G) from the guanine rich homopolymer sequence located at nts 2,085–2,090 (numbering from EcoRI site as position 1) in the HBV core gene. This deletion causes a frameshift generating a premature stop codon at 64Asn in the HBV core gene (codon 93 in the precore gene), that truncates the precore protein, precursor of the secreted hepatitis B “e” antigen (HBeAg), and the core protein which forms the viral nucleocapsid. However, the replication phenotype of the −1G mutant HBV is unknown. An in vitro cell culture model in which hepatoma cells were transiently transfected with infectious cDNAs was used to show that the −1G mutant HBV is incapable of autonomous replication and, as expected, replication was restored to wild‐type (wt) levels by supplying HBV core protein in trans. Although the −1G mutation had no deleterious effect on intracellular HBV‐DNA levels, high levels of −1G mutant HBV relative to wt HBV reduced virus secretion and HBeAg secretion relative to empty vector controls. Importantly, the −1G mutant HBV also caused intracellular retention of truncated precore protein in the endoplasmic reticulum (ER) and Golgi apparatus. Together, these effects may be contributing to the increased pathology observed in the setting of HIV/HBV co‐infection. J. Med. Virol. 84: 1166–1176, 2012. © 2012 Wiley Periodicals, Inc.
Epidemiology of respiratory virus infections among infants and young children admitted to hospital in Oman
Authors: Khamis F.A. , Al‐Kobaisi M.F. , Al‐Areimi W.S. , Al‐Kindi H. , Al‐Zakwani I. 
Published: June 2012

AbstractThe aim of this prospective study was to determine the epidemiology of respiratory viruses responsible for seasonal epidemics of influenza‐like illness in infants and young children in Oman. All children ≤5 years of age consecutively admitted to Sultan Qaboos University Hospital in Oman over a 1‐year period between December 2007 and December 2008 with acute respiratory infections were included. A multiplex polymerase chain reaction (PCR) for viral detection was performed on nasopharyngeal aspirates. Analyses were conducted using univariate statistical methods. Of the 259 infants and young children, at least one respiratory virus was detected in 130 samples (50%). The most prevalent viruses were respiratory syncytial virus (RSV; 43%; n = 56), adenovirus (15%; n = 20), and parainfluenza virus (PIV) (11%; n = 14). Dual or multiple viral infections were found in 23 cases (18%). The three most prominent symptoms of the cohort were fever (78%; n = 201), tachypnoea (77%; n = 200), and runny nose (61%; n = 158). The majority had bronchiolitis (39%; n = 101) while 37% (n = 96) had pneumonia. RSV was more likely to affect those that were young (4 months vs. 7.5 months; P = 0.002) and had tachypnoea (93% vs. 69%; P = 0.004), lower respiratory tract infections (91% vs. 80%; P = 0.039), and bronchiolitis (57% vs. 38%; P = 0.024). The study indicated that respiratory viruses are highly prevalent in children ≤5 years presenting with acute respiratory infections in Oman, of which RSV is the most prominent. J. Med. Virol. 84: 1323–1329, 2012. © 2012 Wiley Periodicals, Inc.
Surface modification counteracts adverse effects associated with immobilization after flexor tendon repair
Authors: Zhao Chunfeng , Sun Yu‐Long , Jay Gregory D. , Moran Steven L. , An Kai‐Nan , Amadio Peter C. 
Published: June 2012

AbstractAlthough post‐rehabilitation is routinely performed following flexor tendon repair, in some clinical scenarios post‐rehabilitation must be delayed. We investigated modification of the tendon surface using carbodiimide derivatized hyaluronic acid and lubricin (cd‐HA‐Lub) to maintain gliding function following flexor tendon repair with postoperative immobilization in a in vivo canine model. Flexor digitorum profundus tendons from the 2nd and 5th digits of one forepaw of six dogs were transected and repaired. One tendon in each paw was treated with cd‐HA‐Lub; the other repaired tendon was not treated. Following tendon repair, a forearm cast was applied to fully immobilize the operated forelimb for 10 days, after which the animals were euthanized. Digit normalized work of flexion (nWOF) and tendon gliding resistance were assessed. The nWOF of the FDP tendons treated with cd‐HA‐Lub was significantly lower than the nWOF of the untreated tendons (p < 0.01). The gliding resistance of cd‐HA‐Lub treated tendons was also significantly lower than that of the untreated tendons (p < 0.05). Surface treatment with cd‐HA‐Lub following flexor tendon repair provides an opportunity to improve outcomes for patients in whom the post‐operative therapy must be delayed after flexor tendon repair. © 2012 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 30:1940–1944, 2012
A single nucleotide polymorphism in microRNA‐146a is associated with the risk for nasopharyngeal carcinoma
Authors: Lung Raymond Wai‐Ming , Wang Xingyan , Tong Joanna Hung‐Man , Chau Shuk‐Ling , Lau Kin‐Mang , Cheng Shuk‐Hang , Woo John Kong‐Sang , Woo Jean , Leung Ping‐Chung , Ng Margaret Heung‐Ling , Tang Nelson Leung‐Sang , To Ka‐Fai 
Published: June 2012

AbstractA common GC polymorphism within miRNA‐146a precursor region (rs2910164) has been associated with the risk of various cancers despite the underlying mechanism is unclear. In the current study, we aimed to examine the role of rs2910164 in the pathogenesis and predisposition to nasopharyngeal carcinoma (NPC). The GC polymorphism in 233 NPC patients, 173 matched controls and 3613 healthy elderly subjects in our locality were first determined using melting temperature (Tm)‐shift allele‐specific genotyping method. Results in our case–control study indicated that CC genotype was associated with the risk effect of NPC (adjusted odds ratio of GC + GG vs. CC, 0.49; 95% confidence interval, 0.35–0.69; P < 0.0001). Using real‐time polymerase chain reaction (PCR) assay, we subsequently revealed that expressions of both miR‐146a and its passenger strand (miR‐146a*C or miR‐146a*G) were increased in NPC samples (P < 0.001), albeit expression of miR‐146a was not linked to the genotype. Furthermore, miR‐146a*C in NPC was significantly increased in CC genotype (CC vs. GC, P = 0.038). Finally, we demonstrated by co‐immunoprecipitation and luciferase reporter assays that all three miR‐146a precursor‐derived mature miRNAs interacted with Argonaute2 (Ago2) protein complex and could function as gene silencers. Taken together, our results showed that the variant C in rs2910164 was associated with the predisposition of NPC in Chinese population. This polymorphism may influence the risk of NPC by producing active mature miR‐146a*C that regulate distinct set of target genes. These findings may enrich our understanding of how miRNA single nucleotide polymorphism affect NPC pathogenesis, and may have potential implications to improve NPC treatment in the future. © 2012 Wiley Periodicals, Inc.
Periodic substrate structure suppresses spurious response in coupled line filters
Authors: Saeidi Chiya , van der Weide Daniel 
Published: June 2012

AbstractPeriodic perturbation of the dielectric medium following Bragg's law and equalizing mode velocities together suppress the first spurious response in coupled‐line filters. The design parameters are calculated using the classical coupled‐line filter design. Fabricating a Chebyshev filter; for the bisected unit cell, spurious response suppression of 35 dB is achieved. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:2175–2178, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.26999
Fast analysis of arbitrary beam through planar multilayer structures
Authors: Liang Bin , Bai Ming , Ou Naiming , Jin Ming , Miao Jungang 
Published: June 2012

AbstractIn this article, a fast and accurate method is presented for analyzing the interaction between arbitrary oriented finite beams over planar multilayer structures.The method is based on Transfer Matrix algorithm and optical fast‐Fourier‐transform based angular spectrum method. Full vectorial near‐fields of electromagnetic beam refracted and reflected by the multilayer can be calculated within several seconds. A numerical simulation in FEKO and an experiment are respectively implemented, and good agreement compared with the hybrid method is obtained. The method can be used in the design process of the multilayer with big amount of parameter adjustment, and then full‐wave simulation can be adopted to get exact results. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:2185–2190, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.27014
Multibias neural modeling of fin field‐effect transistor admittance parameters
Authors: Marinković Zlatica , Crupi Giovanni , Schreurs Dominique M. M.‐P. , Caddemi Alina , Marković Vera 
Published: June 2012

AbstractThe goal of this study is to develop an artificial neural network (ANN) approach to model the fin field‐effect transistor (FinFET) small‐signal admittance parameters. The model is determined for the whole device as well as for the actual transistor, which is obtained after de‐embedding the effects of the probe pads, transmission lines, and substrate. The extracted model is validated in a wide range of operating bias conditions up to 50 GHz. In comparison to the earlier developed ANN‐based model of the FinFET scattering parameters, although the same accuracy is achieved, the proposed technique exhibits the main advantage of a reduced model complexity. © 2012 Wiley Periodicals, Inc. Microwave Opt Technol Lett 54:2082–2088, 2012; View this article online at wileyonlinelibrary.com. DOI 10.1002/mop.27020