bluuebunny/crossref_metadata_2025_split huggingface dataset:
Recognition of RNA secondary structures with a programmable peptide nucleic acid-based platform
Authors: Lu Rongguang , Deng Liping , Lian Yun , Ke Xin , Yang Lixia , Xi Kun , Lerk Ong Alan Ann , Chen Yanyu , Zhou Hanting , Meng Zhenyu , Lin Ruiyu , Fan Shijian , Liu Yining , Toh Desiree-Faye Kaixin , Zhan Xuan , Krishna Manchugondanahalli S. , Patil Kiran M. , Lu Yunpeng , Liu Zheng , Zhu Lizhe , Wang Hongwei , Li Guobao , Chen Gang 
Published: May 2024

SummaryRNA secondary structures comprise double-stranded (ds) and single-stranded (ss) regions. Antisense peptide nucleic acids (asPNAs) enable the targeting of ssRNAs and weakly formed dsRNAs. Nucleobase-modified dsRNA-binding PNAs (dbPNAs) allow for targeting of relatively stable dsRNAs. A programmable RNA structure-specific targeting strategy is needed for simultaneous recognition of dsRNAs and ssRNAs. Here, we report on combining dbPNAs and asPNAs (designated as daPNAs) for the targeting of dsRNA-ssRNA junctions. Our binding and modeling data suggest that combining traditional asPNA (with a 4-letter code: T, C, A, and G) and dbPNA (with a 4-letter code: T or s2U, L, Q, and E) scaffolds facilitates RNA structure-specific tight binding (nM to μM) under physiologically-relevant conditions. We further applied our daPNAs in substrate specific inhibition of Dicer acting on pre-miR-198 in a cell-free assay and regulating ribosomal frameshifting induced by model hairpins in both cell-free and cell culture assays. daPNAs would be a useful platform for developing chemical probes and therapeutic ligands targeting RNA.HighlightWe demonstrated that sequence- and structure-specific targeting of RNA can be facilitated by nucleobase-modified dsRNA-binding PNAs (dbPNAs) platform in combination with antisense PNAs (asPNAs). We name the novel PNAs as daPNAs.daPNAs can be used in a programmable way for targeting RNAs by formation of a short triplex next to a short duplex at a dsRNA-ssRNA junction.We applied our daPNAs in substrate specific inhibition of Dicer acting on pre-miR-198 in a cell-free assay and regulating ribosomal frameshifting induced by model hairpins in both cell-free and cell culture assays.The daPNAs platform would serve as useful junction-specific molecular glues for the targeting of many biologically important RNA structures in transcriptomes.
Tuning single-molecule ClyA nanopore tweezers for real-time tracking of the conformational dynamics of West Nile viral NS2B/NS3 protease
Authors: Shorkey Spencer A. , Zhang Yumeng , Sharp Jacqueline , Clingman Sophia , Nguyen Ly , Chen Jianhan , Chen Min 
Published: May 2024

AbstractThe flaviviral NS2B/NS3 protease is a conserved enzyme required for flavivirus replication. Its highly dynamic conformation poses major challenges but also offers opportunities for antiviral inhibition. Here, we established a nanopore tweezers-based platform to monitor NS2B/NS3 conformational dynamics in real-time. Molecular simulations coupled with electrophysiology revealed that the protease could be captured in the middle of the ClyA nanopore lumen, stabilized mainly by dynamic electrostatic interactions. We designed a newSalmonella typhiClyA nanopore with enhanced nanopore/protease interaction that can resolve the open and closed states at the single-molecule level for the first time. We demonstrated that the tailored ClyA could track the conformational transitions of the West Nile NS2B/NS3 protease and unravel the conformational energy landscape of various protease constructs through population and kinetic analysis. The new ClyA-protease platform paves a way to high-throughput screening strategies for discovering new allosteric inhibitors that target the NS2B and NS3 interface.
Cathepsin X is a conserved cell death protein involved in algal response to environmental stress
Authors: Mizrachi Avia , Sadeh Mai , Ben-Dor Shifra , Dym Orly , Ku Chuan , Feldmesser Ester , Zarfin Amichai , Brunson John K. , Allen Andrew E. , Jinkerson Robert E. , Schatz Daniella , Vardi Assaf 
Published: May 2024

AbstractPhytoplankton play a crucial role in global primary production and can form vast blooms in aquatic ecosystems. Bloom demise and the rapid turnover of phytoplankton are suggested to involve programmed cell death (PCD) induced by diverse environmental stressors. However, fundamental knowledge of the PCD molecular components in algae and protists in general remains elusive. Previously, we revealed that early oxidation in the chloroplast predicted subsequent cell death or survival in isogenic subpopulations that emerged following H2O2treatment in the diatomPhaeodactylum tricornutum. Here, we performed transcriptome analysis of sorted sensitive oxidized cells and resilient reduced cells, to discover genes linked to their contrasting fates. By cross-comparison with a large-scale mutant screen in the green algaChlamydomonas reinhardtii, we identified functionally relevant conserved PCD gene candidates, including the cysteine protease cathepsin X/Z (CPX).CPXmutants inP. tricornutum CPX1andC. reinhardtii CEP12both exhibited profound resilience to oxidative stress, supporting a conserved function in algal PCD.P. tricornutum cpx1mutants, generated using CRISPR-Cas9, also exhibited resilience to the toxic diatom-derived infochemical cyanogen bromide. Phylogenetic and predictive structural analyses show thatCPXis highly conserved in eukaryotes, and algae of the green and red lineages exhibit strong structural similarity to human cathepsinCTSZ.CPXis expressed by diverse algae across the oceans and during toxicPseudo-nitzschiablooms, supporting its ecological importance. Elucidating PCD components in algae sheds light on the evolutionary origin of PCD in unicellular organisms, and on the cellular strategies employed by the population to cope with stressful conditions.
Corporate governance and corporate sustainability performance: the mediating role of CSR expenditure
Authors: Rashid Md Mamunur , Kabir Md Rabiul 
Published: May 2024

PurposeThis study investigates the effect of corporate governance (CG) characteristics on corporate sustainability performance (CSP) and whether the magnitude of CSR expenditure mediates such a relationship in the context of an emerging and developing economy-Bangladesh.Design/methodology/approachThis study collects data from the annual reports of 30 private commercial banks listed with the Dhaka Stock Exchange for the period starting from 2013 to 2022, giving 300 firm-year observations. To test the hypotheses formulated, this study uses Baron and Kenny’s (1986) four-step model. Data have been analyzed using AMOS 23 to examine the direct and indirect effect of CG on sustainability performance.FindingsThis study finds that several CG variables (board size, board independence, sustainable finance committee) significantly affect several facets of sustainability performance (environmental, social, and financial performance). However, the mediating role of the magnitude of CSR expenditure in the relationship between CG mechanisms and sustainability performance is found to be limited.Research limitations/implicationsThe list of CG and ownership structure variables studied is not exhaustive, and the presence of a wide variation in the measurement of sustainability performance makes its measurement subjective to some extent.Originality/valueThis study uses the magnitude of CSR expenditure as a mediator in the relationship between CG and sustainability performance, which is rarely addressed by the extant literature in this field.
Seeding innovation: the role of internal and external digital data in agri-food product innovation
Authors: Frau Moreno , Keszey Tamara 
Published: May 2024

PurposeSince previous literature provides fragmented and conflicting results about the use of digital data for product innovation, the article aims to comprehensively explore and shed light on how agri-food firms utilise external and internal digital data sources when dealing with different product innovations, such as incremental, architecture and radical innovation.Design/methodology/approachThis paper adopts an exploratory multiple-case study and a theory-building process, focussing on the agri-food industry. We collected primary and secondary data from eight manufacturing companies.FindingsThe findings of this research show an empirical framework of six agri-food firms’ digital data utilisation behaviours: the supervisor, the passive supervisor, the developer, the passive developer, the pathfinder and the conjunction behaviour. These digital data utilisation behaviours vary according to a combination of data sources, such as internal data related to inside phenomenon measures (e.g. data generated by sensors installed in the production plan) or external data (e.g., market trends, overall sector sales), and innovation purposes.Practical implicationsThis article offers guiding principles that assist agri-food companies when utilising internal and external digital data sources for specific product innovation outcomes such as incremental, architectural and radical innovation.Originality/valueThe significance of external and internal data sources in stimulating product innovation has garnered substantial attention within academic discussions, highlighting the critical importance of analysing digital data for driving such innovation. Nonetheless, the predominant approach is to study a single innovation outcome through the lens of digital technology. In contrast, our study stands out by adopting a fundamental perspective on data sources, enabling a more nuanced explanation of the overall product innovation outcomes within the agri-food sector.
Driving metaverse adoption in the hospitality industry: an upper echelon perspective
Authors: Agnihotri Arpita , Bhattacharya Saurabh , Sakka Georgia , Vrontis Demetris 
Published: May 2024

Purpose
The purpose of this study is to explore how past and future temporal focus of CEOs in the hospitality industry influence their intention to invest in metaverse technology and the underlying mechanism under boundary conditions of perceived competitive pressure.

Design/methodology/approach
This multi-informant study collected data over three waves from a sample of 235 CEOs and their subordinates in India’s hospitality industry. A PLS-SEM was applied to the study data. Further, the study also used phenomenological interviews to capture CEOs’ perspectives on the study’s conceptual model.

Findings
Findings suggest that the past temporal focus of CEOs decreases technology orientation, and future temporal focus increases the technology orientation of firms, consequently impacting the intention to invest in the metaverse. CEOs’ perceived competitive pressure moderates the mediating relationship, such that the negative impact of past temporal focus on technology orientation is decreased and that of future temporal focus on the CEO is increased.

Research limitations/implications
By exploring the role of a CEO’s past and future temporal focus on influencing technology orientation and, hence, adoption of new technology, the study extends upper-echelon theory to the field of metaverse adoption in the hospitality industry and responds to scholars’ calls to explore the industry’s technology adoption from the lens of the upper echelon.

Practical implications
The study has significant implications for the success of the adoption of metaverse technology in the hospitality industry. Findings imply that the board members should encourage CEOs to have future temporal focus.

Originality/value
The study provides novel insights into the adoption of metaverse technology by the hospitality industry, where CEO attributes such as their temporal focus influence intention to invest in metaverse.
Motivating supervisors during disability accommodation: a comprehensive examination on job demand and resources theories
Authors: Alam Mohammad Shahin , Williams-Whitt Kelly , Shin DuckJung , Ansari Mahfooz 
Published: May 2024

PurposeThis study develops and tests a comprehensive model that examines whether dimensions of supervisors’ job demands and resources influence their work motivation through their job strain levels while managing disability accommodation (DA).Design/methodology/approachThe proposed model leverages the assumptions of established job demand and resources theories, including demand-ability fit, job demand-control, job demand-control-support, and effort-reward balance models. Then, we tested with the quantitative data from 335 British, Canadian, American, Australian, Dutch, and German supervisors with recent DA experience.FindingsThis study found support for the proposed model. Job control and social support directly affected work motivation, while job strain did not mediate the relationship between job control and social support and work motivation. The results suggest that employers looking to improve the likelihood of DA success should focus on providing adequate job control, social support, and rewards to supervisors responsible for accommodating employees with disabilities.Practical implicationsThis research enhances our understanding of how additional DA responsibilities impact supervisors and aids in the development of effective DA management policies and interventions, providing robust support for practitioners.Originality/valueThis study contributes to extending the DA literature by testing the applicability of different theoretical models to explain the effect of the additional DA responsibility on supervisors’ job demand, strain, and motivation levels and identify the resources to mitigate them.
When friends are near foes: governing nonequity alliances with steering committees
Authors: Tseng Chiung-Hui , Lien Nguyen Thi Kim 
Published: May 2024

Purpose
Indirect knowledge leakage to rivals located near alliance partners represents a significant risk that has received limited scholarly attention. Hence, the question of how to manage this risk – which the authors term “partner-rival co-location risk” – in nonequity alliances remains unanswered, and this study aims to suggest establishing a steering committee to oversee the partnership.

Design/methodology/approach
Drawing on the agglomeration economies and alliance governance literatures, the authors develop a set of hypotheses and perform a series of empirical tests on 470 nonequity alliances in the US biopharmaceutical industry.

Findings
The authors propose that there is a positive linkage between partner-rival co-location risk and the formation of a steering committee in a nonequity alliance, which receives strong empirical support. Further, this relationship is significantly moderated by the breadth (alliance scope) but not the depth (reciprocal interdependence) of interaction between the partnering firms.

Originality/value
This paper is a pioneer to shed light on “partner-rival co-location risk” and how partner-rival co-location risk affects the governance decision of whether to establish a steering committee in a nonequity alliance, thus offering important theoretical and practical insights into competition and cooperation in alliance management.
Visualising knowledge: a survey of infographic perceptions and challenges in academic library settings
Authors: Butdisuwan Sujin , Kataria Sanjay , Taj Amreen , Subaveerapandiyan A. 
Published: May 2024

PurposeThis study investigates the perceptions and challenges faced by academic library professionals in utilising infographics as a communication tool. It aims to understand how infographics are perceived in libraries, their effectiveness in conveying information and the obstacles encountered during implementation.Design/methodology/approachThe research employed a descriptive survey design, collecting data through a closed-ended questionnaire distributed electronically to 144 library and information science professionals. Stratified random sampling was used to ensure representation from various types of libraries. Pilot testing was conducted to validate the questionnaire, and data analysis was performed using statistical software.FindingsLibrary professionals perceive infographics as valuable tools for conveying complex concepts quickly, promoting library events and appealingly presenting resources. They also believe that infographics simplify complex information and enhance the user experience. However, challenges include lacking design skills and resources, difficulty finding relevant data, integrating infographics into traditional library services and time constraints.Originality/valueThis study provides unique insights into the role of infographics in library communication, highlighting both their positive perceptions and critical challenges. It offers recommendations for enhancing the use of infographics, including training for library professionals, improved data management and integration into traditional library services. The study also suggests promoting best practices and exploring emerging technologies like 3D interactive infographics.
A diversity of diversities: Do complex environmental effects underpin associations between below‐ and above‐ground taxa?
Authors: Seaton Fiona M. , George Paul B. L. , Alison Jamie , Jones Davey L. , Creer Simon , Smart Simon M. , Emmett Bridget A. , Robinson David A. 
Published: May 2024

Abstract

To predict how biodiversity will respond to global change, it is crucial to understand the relative roles of abiotic drivers and biotic interactions in driving associations between the biodiversity of disparate taxa. It is particularly challenging to understand diversity–diversity links across domains and habitats, because data are rarely available for multiple above‐ and below‐ground taxa across multiple sites.

Here, we analyse data from a unique biodiversity data set gathered across a variety of oceanic temperate terrestrial habitats in Wales, comprising 300 sites with co‐located soil microbial, plant, bird and pollinator surveys along with climate and soil physicochemical information. Soil groups are analysed using metabarcoding of the 16S, ITS1 and 18S DNA regions, allowing in‐depth characterisation of microbial and soil animal biodiversity.

We explore biodiversity relationships along three aspects of community composition: First, we assess correlation between the alpha diversity of different groups. Second, we assess whether biotic turnover between sites is correlated across different groups. Finally, we investigate the co‐occurrence of individual taxa across sites. In each analysis, we assess the contribution of linear or nonlinear environmental effects.

We find that a positive correlation between alpha diversity of plants, soil bacteria, soil fungi, soil heterotrophic protists, bees and butterflies is in fact driven by complex nonlinear responses to abiotic drivers. In contrast, environmental variation did not account for positive associations between the diversity of plants and both birds and AM fungi, suggesting a role for biotic interactions.

Both the diversity and taxon‐level associations between the differing soil groups remained even after accounting for nonlinear environmental gradients. Above‐ground, spatial factors played larger roles in driving biotic communities, while linear environmental gradients were sufficient to explain many group‐ and taxon‐level relationships.

Synthesis. Our results show how nonlinear responses to environmental gradients drive many of the relationships between plant biodiversity and the biodiversity of above‐ and below‐ground biological communities. Our work shows how different aspects of biodiversity might respond nonlinearly to changing environments and identifies cases where management‐induced changes in one community could either influence other taxa or lead to loss of apparent biological associations.
Simplifying, Innovating, and Collaborating: Educating the Health Workforce for Medicare's Middle‐age
Authors: Brock Tina , Davidson Sandra , Molloy Elizabeth 
Published: May 2024

AbstractAustralia's Medicare is at a mid‐life reflection point. This is not a moment for existential dread or rash actions. Instead, it is a time for age‐related improvement. Middle‐aged Medicare would benefit from policy that reflects the benefits of subtractive change to reduce its complexity. In addition, policies prioritising the use of data science to enact technologically innovative approaches would improve overall flexibility. Finally, policy can play an important role in strengthening connections between health profession cadres, incentivising them to work together at the top of scope. With education, practice, and policy collaborating, we can enable Medicare's wellbeing for years to come.
Medicare at 40: Are We Showing Our Age?
Authors: Hall Jane , van Gool Kees , Haywood Philip , Fiebig Denzil 
Published: May 2024

AbstractTo understand what Medicare aimed to achieve, we need to revisit the medico‐politics of the time, and the fear of the spectre of socialised medicine. That determined what could be changed (universal insurance and contributions according to means) and what could not (private medical service provision and fee‐for‐service). We consider what Medicare has achieved in terms of community acceptance, fairer contributions, affordability at its establishment; and how those aims can be assessed today. While Medicare is undoubtedly a success, there are inflexibilities in its structure that are challenging in ensuring it is fit for the next four decades.
Examining the association of hysterectomy with and without oophorectomy on cardiovascular disease and all‐cause, cardiovascular or cancer mortality: A systematic review and meta‐analysis
Authors: Chen Yuge , Li Fengjuan , Liang Lei , Hua Huiling , Liu Shizheng , Yu Zihe , Chen Qiuyu , Huang Shufeng , Qin Pei 
Published: May 2024

AbstractBackgroundThe associations between hysterectomy and cardiovascular disease (CVD) and mortality remains unlcear and a meta‐analysis with cohort studies is lacking.ObjectivesThis study aimed to conduct a systematic review and meta‐analysis of cohort studies to investigate the relationship between hysterectomy and CVD, coronary heart disease (CHD), stroke, heart failure, and all‐cause, cardiovascular and cancer mortality. We further explored the effect of oophorectomy on the association between hysterectomy and these health outcomes.Search strategyPubMed, EMBASE and Web of Science were searched up to 24 July 2023.Selection criteriaCohort studies.Data collection and analysisHazard ratios (HRs) and 95% confidence intervals (95% CIs) were pooled using a random‐effects model. We used I2 to assess the heterogeneity between studies.Main resultsForty‐three studies were included in the meta‐analysis. Hysterectomy was significantly associated with an increased risk of CVD (pooled HR 1.11, 95% CI 1.09–1.13; n = 6; I2 = 0) and stroke (HR 1.09, 95% CI 1.04–1.14; n = 7; I2 = 52%), but with a decreased risk of cancer mortality (HR 0.93, 95% CI 0.86–1.00; n = 4; I2 = 81%). No significant association was observed between hysterectomy and CHD (n = 10; I2 = 83%), all‐cause mortality (n = 8; I2 = 81%) or cardiovascular mortality (n = 7; I2 = 89%). Hysterectomy with and without oophorectomy was significantly associated with CVD and stroke risk, but showed a larger effect size for hysterectomy with oophorectomy. A significantly increased risk of CHD was observed in the subgroup of hysterectomy with oophorectomy, but not for the subgroup of hysterectomy alone.ConclusionsHysterectomy may increase the risk of CVD, CHD and stroke, but not all‐cause, cardiovascular or cancer mortality. Hysterectomy with oophorectomy may have a higher risk of CVD, CHD and stroke than hysterectomy alone. However, the results on CHD and mortality related to hysterectomy should be interpreted cautiously because of the high level of heterogeneity and unstable subgroup analyses.