bluuebunny/crossref_metadata_2025_split huggingface dataset:
The Biomimetic Synthesis and First Characterization of the (+)‐ and (−)‐Isocentrolobines, 2,6‐cis‐ and 2,6‐trans‐Disubstituted Tetrahydro‐2H‐pyrans
Authors: Rogano Frank , Froidevaux Georges , Rüedi Peter 
Published: July 2010

AbstractThe four stereoisomers of the novel title compounds were prepared by oxidative cyclization of their enantiomerically pure diarylheptanoid precursors by means of the straightforward biomimetic approach presented in the preceding article. The isocentrolobines are the methoxy regioisomers of the natural (+)‐ and (−)‐centrolobines and were characterized for the first time. The synthetic procedure established the absolute configurations and the unambiguous correlation with the chiroptical data. The spectroscopic and the chiroptical data of the isocentrolobines are highly similar to those of the natural products. The single diagnostic parameter that would allow a immediate assignment in the presence of only one of the isomers is the higher melting point (ca. 50°) of the cis‐configured isocentrolobines.
Efficient Total Synthesis of Racemic Bisabolane Sesquiterpenes Curcuphenol and Xanthorrhizol Starting from Substituted Acetophenones
Authors: Montiel Luisa E. , Zepeda L. Gerardo , Tamariz Joaquín 
Published: July 2010

AbstractA total synthesis of natural bisabolane sesquiterpenes curcuphenol (1) and xanthorrhizol (2) was developed by using the substituted acetophenones 4 and 5, respectively, as starting materials. The acetyl group of the latter was activated through ethoxycarbonylation to carry out the prenylation, which was performed successfully to give their respective precursors 11a and 11b, and 21 that were converted into the corresponding natural sesquiterpenes 1 and 2, respectively, over four steps and in high overall yields.
Hydriodic Acid‐Mediated Cyclization of α‐Substituted Secondary 2‐Ethenylbenzamides: Synthesis of 2‐Substituted 2,3‐Dihydro‐3,3‐dimethyl‐1H‐isoindol‐1‐ones and 3,3‐Disubstituted (E)‐1‐(Arylimino)‐1,3‐dihydroisobenzofurans
Authors: Kobayashi Kazuhiro , Fujita Seiki , Nakai Daisuke , Fukumoto Shogo , Fukamachi Shuhei , Konishi Hisatoshi 
Published: July 2010

AbstractA new and facile method for the preparation of 2‐substituted 2,3‐dihydro‐3,3‐dimethyl‐1H‐isoindol‐1‐ones 3 and 3,3‐disubstituted (E)‐1‐(arylimino)‐1,3‐dihydroisobenzofurans 6 has been developed. Thus, treatment of N‐alkyl(or aryl)‐2‐(1‐methylethen‐1‐yl)benzamides 2 with concentrated hydriodic acid (HI) in MeCN at room temperature afforded 3. Similar treatment of N‐aryl‐2‐(1‐phenylethen‐1‐yl)benzamide 5 with concentrated HI at 0° afforded 6.
Common alleles in candidate susceptibility genes associated with risk and development of epithelial ovarian cancer
Authors: Notaridou Maria , Quaye Lydia , Dafou Dimitra , Jones Chris , Song Honglin , Høgdall Estrid , Kjaer Susanne K. , Christensen Lise , Høgdall Claus , Blaakaer Jan , McGuire Valerie , Wu Anna H. , Van Den Berg David J. , Pike Malcolm C. , Gentry‐Maharaj Aleksandra , Wozniak Eva , Sher Tanya , Jacobs Ian J. , Tyrer Jonathan , Schildkraut Joellen M. , Moorman Patricia G. , Iversen Edwin S. , Jakubowska Anna , Mędrek Krzysztof , Lubiński Jan , Ness Roberta B. , Moysich Kirsten B. , Lurie Galina , Wilkens Lynne R. , Carney Michael E. , Wang‐Gohrke Shan , Doherty Jennifer A. , Rossing Mary Anne , Beckmann Matthias W. , Thiel Falk C. , Ekici Arif B. , Chen Xiaoqing , Beesley Jonathan ,   The Australian Ovarian Cancer Study Group/Australian Cancer Study (Ovarian Cancer), Gronwald Jacek , Fasching Peter A. , Chang‐Claude Jenny , Goodman Marc T. , Chenevix‐Trench Georgia , Berchuck Andrew , Pearce C. Leigh , Whittemore Alice S. , Menon Usha , Pharoah Paul D.P. , Gayther Simon A. , Ramus Susan J. ,   on behalf of the Ovarian Cancer Association Consortium
Published: July 2010

AbstractCommon germline genetic variation in the population is associated with susceptibility to epithelial ovarian cancer. Microcell‐mediated chromosome transfer and expression microarray analysis identified nine genes associated with functional suppression of tumorogenicity in ovarian cancer cell lines; AIFM2, AKTIP, AXIN2, CASP5, FILIP1L, RBBP8, RGC32, RUVBL1 and STAG3. Sixty‐three tagging single nucleotide polymorphisms (tSNPs) in these genes were genotyped in 1,799 invasive ovarian cancer cases and 3,045 controls to look for associations with disease risk. Two SNPs in RUVBL1, rs13063604 and rs7650365, were associated with increased risk of serous ovarian cancer [HetOR = 1.42 (1.15–1.74) and the HomOR = 1.63 (1.10–1.42), p‐trend = 0.0002] and [HetOR = 0.97 (0.80–1.17), HomOR = 0.74 (0.58–0.93), p‐trend = 0.009], respectively. We genotyped rs13063604 and rs7650365 in an additional 4,590 cases and 6,031 controls from ten sites from the United States, Europe and Australia; however, neither SNP was significant in Stage 2. We also evaluated the potential role of tSNPs in these nine genes in ovarian cancer development by testing for allele‐specific loss of heterozygosity (LOH) in 286 primary ovarian tumours. We found frequent LOH for tSNPs in AXIN2, AKTIP and RGC32 (64, 46 and 34%, respectively) and one SNP, rs1637001, in STAG3 showed significant allele‐specific LOH with loss of the common allele in 94% of informative tumours (p = 0.015). Array comparative genomic hybridisation indicated that this nonrandom allelic imbalance was due to amplification of the rare allele. In conclusion, we show evidence for the involvement of a common allele of STAG3 in the development of epithelial ovarian cancer.
Increased epidermal growth factor receptor expression at the invasive margin is a negative prognostic factor in colorectal cancer
Authors: Ljuslinder Ingrid , Melin Beatrice , Henriksson Maria L. , Öberg Åke , Palmqvist Richard 
Published: July 2010

AbstractThe receptor tyrosine kinase epidermal growth factor receptor (EGFR) is often expressed in solid malignant tumours, and the expression has been correlated to disease progression. Multiple new agents targeted against the EGFR have been developed during the last decade, but treatment selecting criteria are still not clear. This immunohistochemical study includes 386 colorectal cancer patients and focuses on EGFR expression variations within the tumour, comparing central parts to the invasive margin. Positive immunostaining for EGFR was evident in the central part in 176/386 (46%) of analyzed primary tumours. The invasive margin was positive in 222/386 (58%). A similar expression in both the central part and the invasive front was evident in 286/386 (74%). An increased score at the invasive margin compared to central parts (EGFRi) was evident in 97/386 (25%) of the tumours. Moreover, the results show a significant survival disadvantage for the EGFRi group, both in potentially curatively resected colon cancer patients (n = 170, p = 0.01) and in potentially curatively resected colon and rectal cancer patients combined (n = 273, p = 0.013). Multivariate survival analysis adjusted for age, gender, bowel localisation, grade, stage and tumour type showed an increased risk of cancer death for EGFRi tumours (HR, 1.53; 95% CI, 1.04–2.23; p = 0.029). A significant correlation between EGFR expression at the invasive margin and the presence of budding was seen (p = 0.0001). This investigation of a large patient material implies that EGFR immunohistochemical analysis still has a role in risk evaluation of colorectal cancer patients.
Enhancing the action of rituximab by adding fresh frozen plasma for the treatment of fludarabine refractory chronic lymphocytic leukemia
Authors: Xu Wei , Miao Kou‐Rong , Zhu Dan‐Xia , Fang Cheng , Zhu Hua‐Yuan , Dong Hua‐Jie , Wang Dong‐Mei , Wu Yu‐Jie , Qiao Chun , Li Jian‐Yong 
Published: July 2010

AbstractComplement deficiencies have been identified in many chronic lymphocytic leukemia (CLL) patients. Supplying fresh frozen plasma (FFP)‐derived complement can enhance complement‐dependent cell lysis by the rituximab. The objective of our study was to evaluate the clinical efficacy and safety of the treatment by adding FFP to rituximab in fludarabine refractory CLL patients. Twenty‐two patients were treated with two units of FFP followed with rituximab, 375 mg/m2, as a single agent, repeated every 1–2 weeks. Patients received a median of four courses of the combined FFP and rituximab treatment (range: 2–6). Sixteen patients (72.7%) responded to treatment and seven (31.8%) achieved a complete remission. Three (13.6%) of which had no evidence of minimal residual disease after treatment. Patients with high expression of ZAP‐70 or CD38, unmutated immunoglobulin heavy chain variable region, mutated p53, or adverse cytogenetic features, achieved response to treatment at rates that appeared similar to those who did not have such characteristics. With a median follow‐up of 12 (4–19) months, the median overall survival and progression free survival have not been achieved. Toxicity was minimal, and the treatment was well tolerated. Our data suggest that the adding FFP to rituximab is an effective nonmyelotoxic regimen for the treatment of fludarabine refractory CLL patients.
Investigation of polyethylenimine‐grafted‐triamcinolone acetonide as nucleus‐targeting gene delivery systems
Authors: Ma Kun , Hu Minxin , Xie Meng , Shen Haijun , Qiu Liyan , Fan Weimin , Sun Hongying , Chen Shuqing , Jin Yi 
Published: July 2010

AbstractBackgroundNuclear membrane is one of the main barriers in polymer mediated intracellular gene delivery. To improve the transgenic activity and safety of nonviral vector, triamcinolone acetonide (TA) as a nuclear localization signal was conjugated with different molecular weight polyethylenimine (PEI).MethodsDifferent molecular weight PEI [600, 1800, 25 000 (25k)] was conjugated with TA to synthesize PEI‐TA by two‐step reaction. Their physicochemical characteristics, in vitro cytotoxicity and transfection efficiency were evaluated. To investigate the difference of transfection efficiency of various molecular weight PEI‐TA, their transfection mechanism was further investigated by confocal microscopy and competition assay. Transgenic expression in vivo was evaluated by injection into hepatic portal vein of mice.ResultsAll PEI‐TA could form nanosize polyplexes with DNA and their physicochemical properties resemble each other. Their cytotoxicities were negligible compared to PEI 25k. The order of transfection efficiency was PEI 1800‐TA > PEI 600‐TA > PEI 25k‐TA. A transfection mechanism study displayed that TA could inhibit considerably the transgenic activity of PEI 1800‐TA and PEI 600‐TA, but that of PEI 25k‐TA was not inhibited. It was suggested that PEI 1800‐TA and PEI 600‐TA might translocate into the nucleus. Confocal microscopy investigation verified this suggestion. The data strongly suggested that the transfection efficiency of PEI 1800‐TA in vivo was much higher than that of PEI 25k, which was consistent with the results obtained in vitro.ConclusionsLow molecular weight PEI‐TA could translocate into the nucleus efficiently. PEI 1800‐TA presented higher transgenic activity and it has a great potential for gene therapy as a nonviral carrier. Copyright © 2010 John Wiley & Sons, Ltd.
Effects of provider characteristics on care coordination under comanagement
Authors: Hinami Keiki , Whelan Chad T. , Konetzka R. Tamara , Edelson Dana P. , Casalino Lawrence P. , Meltzer David O. 
Published: July 2010

AbstractBACKGROUND:Care coordination is critical in settings characterized by high levels of uncertainty, time constraints, and interdependent work processes. The effects of provider characteristics on coordination in comanaged teams has never been examined.OBJECTIVE:To characterize individual providers based on their contribution to team coordination.PARTICIPANTS:Hospitalists, nonphysician providers, hepatologists, and fellows on a comanaged liver service of an academic hospital.DESIGN:Between April 2008 and October 2008, participants were surveyed at baseline and repeatedly at the completion of physician rotations to assess their preferred and actual comanagement structures. In addition, they repeatedly rated their comanagers' contributions to overall coordination using an instrument that assessed relational coordination (RC). Providers were categorized into tertiles of RC. Their management preferences and the frequency of a “composite bad outcome” (intensive care unit [ICU] transfer or inpatient death) in each tertile were evaluated.RESULTS:All (100%) Baseline Surveys and 177/224 (79%) Repeated Surveys were completed by 32 providers. RC was shown to be a stable attribute of providers and not of adverse patient outcomes. Higher coordinators were characterized by their “ownership of patients” (higher 86% vs. lowest 20%, P < 0.01). High compared to low coordinator hepatologists demonstrated leadership through a broader delegation of tasks as well as self‐assignment of responsibilities. A trend toward more frequent “composite bad outcomes” was seen for low tertile physicians: hospitalists (low 8.6% vs. high 1.1%, P < 0.01), hepatologists (low 5.2% vs. high 2.0%, P = 0.22), fellows (low 5.8% vs. high 1.8%, P = 0.08).CONCLUSION:Individual provider's teamwork‐related disposition affects perceived coordination on comanaged team and may influence patient outcomes. Journal of Hospital Medicine 2010. © 2010 Society of Hospital Medicine.
Quantification of Norwalk virus inocula: Comparison of endpoint titration and real‐time reverse transcription‐PCR methods
Authors: Liu Pengbo , Hsiao Hui‐Mien , Jaykus Lee‐Ann , Moe Christine 
Published: July 2010

AbstractHuman noroviruses (NoV) are the leading cause of epidemic acute gastroenteritis. In order to fully characterize features such as persistence and infectious dose, precise quantification of virus concentration is necessary. The purpose of this study was to compare two methods [endpoint titration RT‐PCR and quantitative RT‐PCR (RT‐qPCR)] with respect to quantification of Norwalk virus (NV) in inocula made from purified stock suspensions of human fecal specimens. A full‐length NV RNA transcript was developed to facilitate quantification using RT‐qPCR and provided log linear detection in the range of 49–4.9 × 104 genome equivalent copies (GEC) per reaction. Endpoint titration RT‐PCR was used to estimate PCR detection units, and RT‐qPCR was used to estimate genome copies in two NV inocula (8fIIa and 8fIIb) used in previous human challenge studies. Overall, RT‐qPCR was 1.1–1.6 log10 more sensitive (lower detection limit) than endpoint titration RT‐PCR when the same RNA release method, PCR primers and thermocycle program were used. These findings have important implications for many experimental interpretations, not the least of which is estimating the median infectious dose in human challenge studies. J. Med. Virol. 82:1612–1616, 2010. © 2010 Wiley‐Liss, Inc.
The prevalence of antibodies to human herpesvirus 8 and hepatitis B virus in patients in two hospitals in Tanzania
Authors: Meschi S. , Schepisi M. Sañé , Nicastri E. , Bevilacqua N. , Castilletti C. , Sciarrone M.R. , Paglia M.G. , Fumakule R. , Mohamed J. , Kitwa A. , Mangi S. , Molteni F. , Di Caro A. , Vairo F. , Capobianchi M.R. , Ippolito G. 
Published: July 2010

AbstractThe aim of this study was to determine the seroprevalence of human herpesvirus 8 (HHV‐8) and the immunization status for hepatitis B virus (HBV) infection in febrile patients in two districts of the United Republic of Tanzania. Between February and March 2007, blood samples were collected in Pemba Island and Tosamaganga from 336 outpatients and sent to the Virology Laboratory in Rome (Italy) for testing. HHV‐8 DNA and HBV‐DNA were amplified by two in‐house molecular methods, anti‐HHV‐8 antibody titers were determined by an immunofluorescence assay (IFA), and anti‐HCV, HBsAg, anti‐HBs, and anti‐HBc were evaluated by microplate enzyme immunoassay (MEIA). The seroprevalence of HHV‐8 was 30.7% (96/313). In Pemba Island, the prevalence was lower than in Tosamaganga (14.4% vs. 46.3%). A higher prevalence of low titers of HHV‐8 IgG (<1:80, 81%) was found among those under 5 years of age. HHV‐8 DNA was detected in six seropositive patients (6.7%). The prevalence of HBsAg, anti‐HBs, and anti‐HBc was 4.3%, 37.6%, and 29.3%, respectively. Out of 277 patients, 70 had had a previous infection (25.3%). One case of occult hepatitis was found. The cover of hepatitis B vaccination was higher among children born after 2002 (66.7%) than in patients born before 2002. HHV‐8 infection is endemic in Tanzania and the seroprevalence rate was higher in the mainland than on Pemba Island. The 3.9% percentage of HBsAg in children younger than 4 years of age suggests that increased efforts are required in order to achieve universal and compulsory immunization of children against HBV. J. Med. Virol. 82:1569–1575, 2010. © 2010 Wiley‐Liss, Inc.
The prevalence of “anti‐HBc alone” and HBV DNA detection among anti‐HBc alone in Korea
Authors: Kang So‐Young , Kim Myeong‐Hee , Lee Woo‐In 
Published: July 2010

AbstractThe “anti‐HBc alone” is a frequent serological finding in clinical laboratories, making it difficult to determine whether the HBV infection has resolved. The objectives of this study were to investigate the prevalence of anti‐HBc alone and HBV DNA detection (occult HBV infection) among anti‐HBc alone, and to describe the demographic and clinical characteristics of anti‐HBc alone. A total of 17,677 sera referred from the Health Promotion Center (HPC group, 4,014 sera) as well as all the hospital clinical departments (Patient group, 13,663 sera) were tested for HBs Ag, anti‐HBc, and anti‐HBs. HBV DNA test using real‐time PCR was performed on 230 anti‐HBc alone. The prevalence of anti‐HBc alone was 8.9%, significantly higher in the Patient group than in the HPC group. The prevalence of anti‐HBc was higher in men than women and was increased with age. Very low levels of HBV DNA were found in only 4 (1.7%) out of 230 subjects with anti‐HBc alone. They were patients with conditions unrelated to chronic liver disease. Considering the high prevalence of anti‐HBc alone, the frequency of occult HBV infection among anti‐HBc alone was unexpectedly low. In addition, HBV viral load was low in these patients. Further studies are required to determine the clinical significance and infectivity of anti‐HBc alone, in conjunction with very low levels of HBV DNA and to standardize the detection methodology for both anti‐HBc alone and HBV DNA. J. Med. Virol. 82:1508–1514, 2010. © 2010 Wiley‐Liss, Inc.
Dynamical simulation of Indian summer monsoon circulation, rainfall and its interannual variability using a high resolution atmospheric general circulation model
Authors: Ratna Satyaban Bishoyi , Sikka D. R. , Dalvi Mohit , Venkata Ratnam J. 
Published: July 2010

AbstractThis paper discusses the simulations of Indian summer monsoon (ISM) using a high‐resolution National Center for Environmental Prediction (NCEP) T170/L42 model for a 20‐year period (1985–2004) with observed Sea Surface Temperature (SSTs) as boundary conditions and using five initial conditions in the first week of May. Good agreement is found between the observed and simulated climatologies. Interannual variability (IAV) of the ISM rainfall as simulated in individual ensemble members and as provided by ensemble average shows that the two series are found to agree well; however, the simulation of the actual observed year‐to‐year variability is poor. The model simulations do not show much skill in the simulation of drought and excess monsoon seasons. One aspect which has emerged from the study is that where dynamical seasonal prediction has specific base for the large areal and temporal averages, the technique is not to be stretched for application on short areal scale such as that of a cluster of a few grid point. Monsoon onset over Kerala (MOK) coast of India and advance from Kerala coast to northwest India is discussed based on ensemble average and individual ensemble member basis. It is suggested that the model is capable of realistically simulating these processes, particularly if ensemble average is used, as the intermember spread in the ensemble members is large. In short, the high‐resolution model appears to provide better climatology and its magnitude of IAV, which compares favourably with observations, although year‐to‐year matching of the observed and simulated seasonal/monthly rainfall totals for India as a whole is not good. Copyright © 2010 Royal Meteorological Society
Evaluating travel website motivational communication using a structural equation modelling approach
Authors: Kah Junghye A. , Lee Choong‐Ki , Chung Namho 
Published: July 2010

AbstractThis study examines online information value structure and its motivational communication competence using the motivational language theory (MLT). MLT explains how communication acts directed by managers to employees generate a greater motivation for work. Five information value dimensions  —  functional, hedonic, innovation, aesthetic and sign  —  were identified through literature on the subject of MLT and applied to online information. This study confirmed the multidimensionality of online information and proposed four online information dimensions: experiential (hedonic–innovation), sign, functional and aesthetic. The findings also suggested that experiential (hedonic–innovation) and functional information values have significant effects for travel motivation, including exploration, family togetherness, novelty, escape and socialisation. Copyright © 2010 John Wiley & Sons, Ltd.
Protein Kinase Target Discovery From Genome‐Wide Messenger RNA Expression Profiling
Authors: Ma'ayan Avi , He John C. 
Published: July 2010

AbstractGenome‐wide messenger RNA profiling provides a snapshot of the global state of the cell under different experimental conditions such as diseased versus normal cellular states. However, because measurements are in the form of quantitative changes in messenger RNA levels, such experimental data does not provide direct understanding of the regulatory molecular mechanisms responsible for the observed changes. Identifying potential cell signaling regulatory mechanisms responsible for changes in gene expression under different experimental conditions or in different tissues has been the focus of many computational systems biology studies. Most popular approaches include promoter analysis, gene ontology, or pathway enrichment analysis, as well as reverse engineering of networks from messenger RNA expression data. Here we present a rational approach for identifying and ranking protein kinases that are likely responsible for observed changes in gene expression. By combining promoter analysis; data from various chromatin immunoprecipitation studies such as chromatin immunoprecipitation sequencing, chromatin immunoprecipitation coupled with paired‐end ditag, and chromatin immunoprecipitation‐on‐chip; protein‐protein interactions; and kinase‐protein phosphorylation reactions collected from the literature, we can identify and rank candidate protein kinases for knock‐down, or other types of functional validations, based on genome‐wide changes in gene expression. We describe how protein kinase candidate identification and ranking can be made robust by cross‐validation with phosphoproteomics data as well as through a literature‐based text‐mining approach. In conclusion, data integration can produce robust candidate rankings for understanding cell regulation through identification of protein kinases responsible for gene expression changes, and thus rapidly advancing drug target discovery and unraveling drug mechanisms of action. Mt Sinai J Med 77:345–349, 2010. © 2010 Mount Sinai School of Medicine