bluuebunny/crossref_metadata_2025_split huggingface dataset:
Increased vitamin D‐driven signalling and expression of the vitamin D receptor, MSX2, and RANKL in tooth resorption in cats
Authors: Booij‐Vrieling Henriëtte E. , Ferbus Didier , Tryfonidou Marianna A. , Riemers Frank M. , Penning Louis C. , Berdal Ariane , Everts Vincent , Hazewinkel Herman A. W. 
Published: February 2010

Booij‐Vrieling HE, Ferbus D, Tryfonidou MA, Riemers FM, Penning LC, Berdal A, Everts V, Hazewinkel HAW. Increased vitamin D‐driven signalling and expression of the vitamin D receptor, MSX2, and RANKL in tooth resorption in cats. Eur J Oral Sci 2010; 118: 39–46. © 2010 The Authors. Journal compilation© 2010 Eur J Oral SciTooth resorption occurs in 20–75% of cats (Felis catus). The aetiology is not known, but vitamin D is suggested to be involved. Vitamin D acts through a nuclear receptor (VDR) and increases the expression of receptor activator of nuclear factor‐κB ligand (rankl) and muscle segment homeobox 2 (msx2) genes. Mice lacking the muscle segment homeobox 2 (msx2) gene show decreased levels of rankl, suggesting an interaction among VDR, MSX2, and RANKL. Here, we investigated the expression of VDR, MSX2, and RANKL proteins, and the activity of the VDR‐mediated signalling pathway (using the quantitative polymerase chain reaction on VDR target genes), in tooth resorption, and measured the serum levels of vitamin D metabolites in cats. Tooth resorption was categorized into either resorptive or reparative stages. In the resorptive stage, odontoclasts expressed MSX2 and RANKL (100% and 88%, respectively) and fibroblasts expressed VDR and MSX2 (both at 100%), whereas fibroblasts expressed RANKL in only 29% of the sites analysed. In the reparative stage, cementoblasts expressed VDR, MSX2, and RANKL, whereas fibroblasts expressed VDR and MSX2, but not RANKL. The vitamin D status did not differ between the groups, based on the serum levels of 25‐hydroxycholecalciferol. However, increased expression of VDR protein, and the relative gene expression levels of 1α‐hydroxylase and the VDR‐target gene, 24‐hydroxylase, indicated the involvement of an active vitamin D signalling in the pathophysiology of tooth resorption in cats.
Preoperative Diagnostic for Palatal Implants: Is CT or CBCT Necessary?
Authors: Jung Britta A. , Wehrbein Heinrich , Wagner Wilfried , Kunkel Martin 
Published: February 2010

ABSTRACTObjective: To evaluate (a) the diagnostic value of lateral radiographs and (b) whether computed tomography (CT) or cone beam computed tomography (CBCT) is necessary in preoperative diagnostics for orthodontic anchorage implants.Patients and Methods: We reviewed all patients who had presented for insertion of a palatal implant between January 2003 and December 2007 at the University Hospital Mainz. On the basis of lateral radiographs, the palatal bone was assessed as follows: (a) sufficient (bone height > 4 mm in the implant axis), (b) ambiguous, or (c) insufficient (bone height < 4 mm in the implant axis). In group A the surgical insertion procedure was performed without further radiological investigation. Group C required other types of anchorage. In cases of an ambiguous bone situation (group B), further diagnostic procedures (CT/CBCT) were performed.Results: During the observation period, 105 patients were screened. Fourteen patients opted for alternative treatment leaving 91 patients for final evaluation. In 89 patients (97.8%), the lateral radiographs showed sufficient bone in the vertical dimension. In all of these cases, the availability of sufficient bone was confirmed intraoperatively. Further investigations were performed in two patients (2.2%) of group B (one CT, one CBCT). Finally, one patient had insufficient bone whereas the second had sufficient bone.Conclusions: Nearly 98% of the patients included in this study had sufficient bone for palatal implant insertion. Lateral radiographs permit correct and reliable evaluation of the quantity of bone in preoperative diagnosis of palatal implants. Additional imaging (CT or CBCT) is only required in rare cases of borderline dimensions.
The ubiquitin ligase Itch mediates the antiapoptotic activity of epidermal growth factor by promoting the ubiquitylation and degradation of the truncated C‐terminal portion of Bid
Authors: Azakir Bilal A. , Desrochers Guillaume , Angers Annie 
Published: February 2010

The truncated C‐terminal portion of Bid (tBid) is an important intermediate in ligand‐induced apoptosis. tBid has been shown to be sensitive to proteasomal inhibitors and downregulated by activation of the epidermal growth factor (EGF) pathway. Here, we provide evidence that tBid is a substrate of the ubiquitin ligase Itch, which can specifically interact with and ubiquitinate tBid, but not intact Bid. Consistently, overexpression of Itch increases cell survival and inhibits caspase 3 activity, whereas downregulation of Itch by RNA interference has the opposite effect, increasing cell death and apoptosis. Treatment with EGF increases Itch phosphorylation and activity, and Itch expression is important for the ability of EGF to increase cell survival after tumour necrosis factor‐related apoptosis‐inducing ligand treatment. Our findings identify Itch as a key molecule between EGF signalling and resistance to apoptosis through downregulation of tBid, providing further details on how EGF receptor and proteasome inhibitors can contribute to the induction of apoptosis and the treatment of cancer.Structural digital abstract
MINT‐7542954: ITCH (uniprotkb:Q96J02) physically interacts (MI:0915) with tBid (uniprotkb:P70444) by anti tag coimmunoprecipitation (MI:0007)
MINT‐7542970: tBid (uniprotkb:P70444) physically interacts (MI:0915) with Ubiquitin (uniprotkb:P62988) by anti tag coimmunoprecipitation (MI:0007)
MINT‐7542986: ITCH (uniprotkb:Q96J02) physically interacts (MI:0915) with tBid (uniprotkb:P70444) by bioluminescence resonance energy transfer (MI:0012)
Cytological Events during Zygote Formation of the Fern Ceratopteris thalictroides
Authors: Cao Jian‐Guo , Wang Quan‐Xi , Yang Nai‐Ying , Bao Wen‐Mei 
Published: February 2010

Abstract The cytological events, including nuclear fusion, digestion of male organelles and rebuilding of the plasmalemma and cell wall, during zygote formation of the fern Ceratopteris thalictroides (L.) Brongn. are described based on the observations of transmission electron microscopy. When the spermatozoid enters the egg and contacts the cytoplasm, the male chromatin relaxes continually. The microtubular ribbon (MTr) is separated from the male nucleus and then an envelope reappears around the male nucleus. During nuclear fusion, the egg nucleus becomes highly irregular and extends some nuclear protrusions. It is proposed that the protrusions fuse with the male nucleus actively. After nuclear fusion the irregular zygotic nucleus contracts gradually. It becomes spherical before the zygote divides. The male chromatin is identifiable as fibrous structure in the zygotic nucleus in the beginning, but it gradually becomes diffused completely. The male organelles, including the MTr, multilayered structure, flagella and the male mitochondria are finally digested in the zygotic cytoplasm. Finally a new plasmalemma and cell wall are formed outside the protoplast. The organelles in the zygote are rearranged, which produces a horizontal polarity zygote. The zygote divides with an oblique‐vertical cell plate facing the apical notch of the gametophyte.
Repolarization of the cardiac action potential. Does an increase in repolarization capacity constitute a new anti‐arrhythmic principle?
Authors: Grunnet M. 
Published: February 2010

AbstractThe cardiac action potential can be divided into five distinct phases designated phases 0–4. The exact shape of the action potential comes about primarily as an orchestrated function of ion channels. The present review will give an overview of ion channels involved in generating the cardiac action potential with special emphasis on potassium channels involved in phase 3 repolarization. In humans, these channels are primarily Kv11.1 (hERG1), Kv7.1 (KCNQ1) and Kir2.1 (KCNJ2) being the responsible α‐subunits for conductingIKr,IKsandIK1. An account will be given about molecular components, biophysical properties, regulation, interaction with other proteins and involvement in diseases. Both loss and gain of function of these currents are associated with different arrhythmogenic diseases. The second part of this review will therefore elucidate arrhythmias and subsequently focus on newly developed chemical entities having the ability to increase the activity ofIKr,IKsandIK1. An evaluation will be given addressing the possibility that this novel class of compounds have the ability to constitute a new anti‐arrhythmic principle. Experimental evidence fromin vitro,ex vivoandin vivosettings will be included. Furthermore, conceptual differences between the short QT syndrome andIKractivation will be accounted for.
Palestinian Water II: Climate Change and Land Use
Authors: Hassan Marwan A. , Shahin Khaled , Klinkenberg Brian , McIntyre Graham , Diabat Mousa , Al‐Rahman Tamimi Abed , Nativ Ronit 
Published: February 2010

AbstractFuture predictions regarding the effects of climate change on water resources are complex and therefore inherently uncertain. However, the conclusions presented in most studies on the subject indicate that current water‐poor regions such as the Middle East will experience even greater water stress with climate change. In this article we find that Palestinian and Israeli water managers must plan for future water crises that will likely result from the combined effects of climate change and increasing urbanization that results from an exponential population growth. Climate change will likely increase water stress in the region through the processes of increasing temperature, decreasing and erratic precipitation and reduced overall aquifer replenishment. Urbanization will further strain freshwater supplies by negatively impacting the quality and quantity of available freshwater in an increasingly populated urban environment. In spite of any inherent uncertainty, water managers in the region need to consider the long‐term effects of both urbanization and climate change in any future water management scheme.
The Geographies of Poverty and Welfare
Authors: Milbourne Paul 
Published: February 2010

AbstractThis article provides a critical review of recent geographical work on the themes of poverty and welfare. It begins with a discussion of the historical development of welfare geography and the recent resurgence of interest in welfare geographies within and beyond the discipline. Attention then shifts to the definitional complexities of poverty and, in particular, some of the difficulties associated with conventional labellings of poverty, poor people and poor places. The next section of the paper explores the relations between poverty, space and place with reference to recent work on the changing spatial distributions of poverty, socio‐spatial forms of polarisation and the complex relations between poverty and place. This is followed by a discussion of welfare geographies, within which the spatial underpinnings and impacts of recent programmes of welfare reform are considered. The article ends by pointing to some new geographical agendas for welfare research.
Geographies of International Trade: Theory, Borders, and Regions
Authors: Andresen Martin A. 
Published: February 2010

AbstractAs old as civilization itself, international trade is fundamentally important to the economic well‐being of nations and their regions. As with any other economic activity, international trade has a geography. Despite this geographic nature of international trade, it is a relatively unexplored topic by economic geographers. This is not to say that economic geographers do not investigate international economic activity, only that they do not study the more general processes of international trade. In this paper, I review three facets of international trade that imply the importance of geography undertaken by economists and geographers: theory, borders, and regions. Recent theoretical research implicates the importance of geography as an integral component of trade theory; the political geography of trade (borders) is shown to have differential effects in different regions; and the formation of regional trading blocs reveals that geography is still important for understanding international relationships. All three of these facets are fundamentally geographical. It is argued here that the discipline of economic geography must broaden its scope in order to apply a geographical imagination to the understanding of international trade.
To Bravo or not? A comparison of wireless esophageal pH monitoring and conventional pH catheter to evaluate non‐erosive gastroesophageal reflux disease in a multiracial Asian cohort
Authors: ANG Daphne , TEO Eng Kiong , ANG Tiing Leong , ONG Jeannie , POH Choo Hean , TAN Jessica , FOCK Kwong Ming 
Published: February 2010

OBJECTIVE:  Non‐erosive reflux disease (NERD) constitutes the majority of patients with gastroesophageal reflux disease (GERD). Esophageal pH monitoring is useful in distinguishing patients with NERD from functional heartburn. The gastroenterologist often faces the dilemma of choosing the most appropriate investigative modality. The wireless Bravo® capsule allows prolonged 48 hour monitoring with improved patient tolerance, but concerns regarding its reduced sensitivity compared to conventional pH catheter have been raised. We compared the prevalence of high esophageal acid exposure and positive symptom correlation profiles (using the symptom index [SI] and symptom association probability [SAP]) in patients who underwent Bravo® compared to patients who underwent conventional pH catheter, and evaluated the efficacy of Bravo® monitoring in a multiracial Asian cohort.METHODS:  Retrospective analysis of all pH studies performed between January 2004 and February 2009 for patients with persistent reflux symptoms and a normal gastroscopy.RESULTS:  66 (27 Male, 42.4 ± 13.4 years) and 55 (24 Male, 47.1 ± 13.3 years) patients underwent wireless and pH catheter evaluation respectively. “True NERD” (abnormal acid exposure) was diagnosed in 26 (39.4%) and 20 (36.4%) patients (pNS) while “acid‐sensitive esophagus” (SI ≥ 50% and/or SAP ≥ 95%) occurred in 14 (21.2%) and 12 (21.8%) patients (pNS) using the wireless and pH catheter respectively. Extended recording time with Bravo® led to an incremental diagnostic yield of 30%.CONCLUSION:  The wireless capsule was well tolerated. The diagnostic yield was similar using both modalities. With the increasing availability of impedance‐pH technology, it is uncertain if devices that detect only acid‐reflux events will be surpassed.
Ca2+-dependent induction of conidiation in submerged cultures of Trichoderma viride
Authors: Šimkovič Martin , Ditte Peter , Kurucová Anita , Lakatoš Boris , Varečka L’udovít 
Published: February 2010

The presence of Ca2+ (up to 0.1 mol/L) in the cultivation media was found to induce the formation of conidia in submerged mycelia of Trichoderma viride in a concentration-dependent manner. Ca2+ dramatically stimulated conidiation after 70 h of cultivation. The effect was present in the dark, and illumination stimulated it only marginally. Low (less than 100 μmol/L) Ca2+ concentrations induced the formation of chlamydospores. Sr2+ could substitute Ca2+ in conidiogenesis with lower efficiency (almost 2 orders of magnitude), while the efficiency of Mg2+, Mn2+, or Ba2+ was lower by almost 3 orders of magnitude. Our results demonstrate that mycelial Ca2+ homeostasis has powerful effects on the conidiation and mycelial morphogenesis in T. viride, and they suggest that there is an additional mechanism of conidiation in addition to those induced by light and starvation.
Some New Weakly Contractive Type Multimaps and Fixed Point Results in Metric Spaces
Authors: Latif Abdul , Abdou Afrah A. N. 
Published: February 2010

AbstractSome new weakly contractive type multimaps in the setting of metric spaces are introduced, and we prove some results on the existence of fixed points for such maps under certain conditions. Our results extend and improve several known results including the corresponding recent fixed point results of Pathak and Shahzad (2009), Latif and Abdou (2009), Latif and Albar (2008), Cirić (2008), Feng and Liu (2006), and Klim and Wardowski (2007).
Transcription Factor KLF11 Integrates Progesterone Receptor Signaling and Proliferation in Uterine Leiomyoma Cells
Authors: Yin Ping , Lin Zhihong , Reierstad Scott , Wu Ju , Ishikawa Hiroshi , Marsh Erica E. , Innes Joy , Cheng Youhong , Pearson Kerry , Coon John Sayler , Kim J. Julie , Chakravarti Debabrata , Bulun Serdar E. 
Published: February 2010

Abstract
Uterine leiomyoma is the most common tumor of the female genital tract and the leading cause of hysterectomy. Although progesterone stimulates the proliferation of uterine leiomyoma cells, the mechanism of progesterone action is not well understood. We used chromatin immunoprecipitation (ChIP)–cloning approach to identify progesterone receptor (PR) target genes in primary uterine leiomyoma smooth muscle cells. We identified 18 novel PR-binding sites, one of which was located 20.5 kb upstream of the transcriptional start site of the Krüppel-like transcription factor 11 (KLF11) gene. KLF11 mRNA levels were minimally downregulated by progesterone but robustly upregulated by the progesterone antagonist RU486. Luciferase reporter assays showed significant baseline and RU486-inducible promoter activity in the KLF11 basal promoter or distal PR-binding region, both of which contained multiple Sp1-binding sequences but lacked classic progesterone response elements. RU486 stimulated recruitment of Sp1, RNA polymerase II, PR, and the coactivators SRC-1 and SRC-2 to the distal region and basal promoter. siRNA knockdown of PR increased KLF11 expression, whereas knockdown of KLF11 increased leiomyoma cell proliferation and abolished the antiproliferative effect of RU486. In vivo, KLF11 expression was significantly lower in leiomyoma tissues compared with adjacent myometrial tissues. Taken together, using a ChIP-cloning approach, we uncovered KLF11 as an integrator of PR signaling and proliferation in uterine leiomyoma cells. Cancer Res; 70(4); 1722–30
miR-146a Suppresses Invasion of Pancreatic Cancer Cells
Authors: Li Yiwei , VandenBoom Timothy G. , Wang Zhiwei , Kong Dejuan , Ali Shadan , Philip Philip A. , Sarkar Fazlul H. 
Published: February 2010

Abstract
The aggressive course of pancreatic cancer is believed to reflect its unusually invasive and metastatic nature, which is associated with epidermal growth factor receptor (EGFR) overexpression and NF-κB activation. MicroRNAs (miRNA) have been implicated in the regulation of various pathobiological processes in cancer, including metastasis in pancreatic cancer and in other human malignancies. In this study, we report lower expression of miR-146a in pancreatic cancer cells compared with normal human pancreatic duct epithelial cells. Reexpression of miR-146a inhibited the invasive capacity of pancreatic cancer cells with concomitant downregulation of EGFR and the NF-κB regulatory kinase interleukin 1 receptor–associated kinase 1 (IRAK-1). Cellular mechanism studies revealed crosstalk between EGFR, IRAK-1, IκBα, NF-κB, and MTA-2, a transcription factor that regulates metastasis. Treatment of pancreatic cancer cells with the natural products 3,3′-diinodolylmethane (DIM) or isoflavone, which increased miR-146a expression, caused a downregulation of EGFR, MTA-2, IRAK-1, and NF-κB, resulting in an inhibition of pancreatic cancer cell invasion. Our findings reveal DIM and isoflavone as nontoxic activators of a miRNA that can block pancreatic cancer cell invasion and metastasis, offering starting points to design novel anticancer agents. Cancer Res; 70(4); 1486–95
Serologic Markers of Effective Tumor Immunity against Chronic Lymphocytic Leukemia Include Nonmutated B-Cell Antigens
Authors: Marina Ovidiu , Hainz Ursula , Biernacki Melinda A. , Zhang Wandi , Cai Ann , Duke-Cohan Jonathan S. , Liu Fenglong , Brusic Vladimir , Neuberg Donna , Kutok Jeffery L. , Alyea Edwin P. , Canning Christine M. , Soiffer Robert J. , Ritz Jerome , Wu Catherine J. 
Published: February 2010

Abstract
Patients with chronic lymphocytic leukemia (CLL) who relapse after allogeneic transplant may achieve durable remission following donor lymphocyte infusion (DLI), showing the potency of donor-derived immunity in eradicating tumors. We sought to elucidate the antigenic basis of the effective graft-versus-leukemia (GvL) responses associated with DLI for the treatment of CLL by analyzing the specificity of plasma antibody responses developing in two DLI-treated patients who achieved long-term remission without graft-versus-host disease. By probing high-density protein microarrays with patient plasma, we discovered 35 predominantly intracellular antigens that elicited high-titer antibody reactivity greater in post-DLI than in pre-DLI plasma. Three antigens—C6orf130, MDS032, and ZFYVE19—were identified by both patients. Along with additional candidate antigens DAPK3, SERBP1, and OGFOD1, these proteins showed higher transcript and protein expression in B cells and CLL cells compared with normal peripheral blood mononuclear cells. DAPK3 and the shared antigens do not represent minor histocompatibility antigens, as their sequences are identical in both donor and tumor. Although ZFYVE19, DAPK3, and OGFOD1 elicited minimal antibody reactivity in 12 normal subjects and 12 chemotherapy-treated CLL patients, 5 of 12 CLL patients with clinical GvL responses were serologically reactive to these antigens. Moreover, antibody reactivity against these antigens was temporally correlated with clinical disease regression. These B-cell antigens represent promising biomarkers of effective anti-CLL immunity. Cancer Res; 70(4); 1344–55
Genomic and Gene Expression Profiling Defines Indolent Forms of Mantle Cell Lymphoma
Authors: Fernàndez Verònica , Salamero Olga , Espinet Blanca , Solé Francesc , Royo Cristina , Navarro Alba , Camacho Francisca , Beà Sílvia , Hartmann Elena , Amador Virginia , Hernández Luis , Agostinelli Claudio , Sargent Rachel L. , Rozman Maria , Aymerich Marta , Colomer Dolors , Villamor Neus , Swerdlow Steven H. , Pileri Stefano A. , Bosch Francesc , Piris Miguel A. , Montserrat Emili , Ott German , Rosenwald Andreas , López-Guillermo Armando , Jares Pedro , Serrano Sergi , Campo Elías 
Published: February 2010

Abstract
Mantle cell lymphoma (MCL) is typically a very aggressive disease with poor outcomes, but some cases display an indolent behavior that might not necessitate treatment at diagnosis. To define molecular criteria that might permit recognition of such cases, we compared the clinicopathologic features, gene expression, and genomic profile of patients who had indolent or conventional disease (iMCL or cMCL). Patients with iMCL displayed nonnodal leukemic disease with predominantly hypermutated IGVH and noncomplex karyotypes. iMCL and cMCL shared a common gene expression profile that differed from other leukemic lymphoid neoplasms. However, we identified a signature of 13 genes that was highly expressed in cMCL but underexpressed in iMCL. SOX11 was notable in this signature and we confirmed a restriction of SOX11 protein expression to cMCL. To validate the potential use of SOX11 as a biomarker for cMCL, we evaluated SOX11 protein expression in an independent series of 112 cases of MCL. Fifteen patients with SOX11-negative tumors exhibited more frequent nonnodal presentation and better survival compared with 97 patients with SOX11-positive MCL (5-year overall survival of 78% versus 36%, respectively; P = 0.001). In conclusion, we defined nonnodal presentation, predominantly hypermutated IGVH, lack of genomic complexity, and absence of SOX11 expression as qualities of a specific subtype of iMCL with excellent outcomes that might be managed more conservatively than cMCL. Cancer Res; 70(4); 1408–18