bluuebunny/crossref_metadata_2025_split huggingface dataset:
Monitoring Mouse Surface Temperature During Stress with a Thermal Camera: A Low‐Cost Infrared Videography System for Evaluating Murine Metabolism
Authors: Page Breanna , Cora Carolina , Reilly James , Reno Ryan , Harbi Wadak , Lynes Maureen S. , Lynes Michael A. , Lynes Matthew D. 
Published: February 2025

AbstractEnergy is required for life, and organisms obtain their energy from fuel sources to enable both anabolic and catabolic processes. Some of this energy is radiated as heat, which can be quantified as a measure of metabolic rate. In some cases, environmental toxicants can alter metabolic energy in undesirable ways, and characterization of new pharmaceuticals can determine the efficacy of desirable metabolic rate manipulation or identify off‐target adverse effects. Current methods to directly measure heat production in laboratory mice are expensive, can be laborious, and make it challenging to monitor animals in ways that are multiplexed, robust, and non‐invasive. We present a set of protocols for assembling and deploying a simple, low‐cost thermal camera to monitor and record thermogenic activity, modified from prior work. Parts used to build this system currently cost approximately $150 USD and, when assembled, can record mouse temperatures as well as ambient cage temperatures up to twice per second for extended periods. By using multiplexed cameras in a diurnal mouse incubator system, the thermogenic capacity of several mice can be simultaneously recorded and graphed. Exogenous agents and genotypes that alter metabolism can be readily identified with this technology. In this set of protocols, we describe the assembly of the thermal video camera device, its use, and related data capture and analysis methods. © 2025 The Author(s). Current Protocols published by Wiley Periodicals LLC.Basic Protocol 1: Assembling thermal camera for thermogenic stress testBasic Protocol 2: In vivo measurement of mouse temperature under different ambient conditions
Phylogenetic Assessment of Gazella bennettii: A Genetic Framework for the Conservation of the Endangered Jebeer in Iran
Authors: Fadakar Davoud , Malekian Mansoureh , Hemami Mahmoud Reza , Rezaei Hamid Reza , Lerp Hannes , Bärmann Eva V. 
Published: February 2025

ABSTRACTThe chinkara (Gazella bennettii, Sykes 1831) exhibits a broad distribution from Iran to India and has been categorized into five species: G. bennettii, G. christyi, and G. salinarum representing the Indian chinkara, and G. fuscifrons and G. shikarii pertaining to the Iranian chinkara (jebeer). This classification within the “Gazelle bennettii group” is solely based on morphological data, lacking genetic information. To investigate the potential presence of multiple species within the group and to determine subspecific variations, we sampled jebeer in Iran and conducted phylogenetic analyses using cytochrome b, COI, and sequences from two nuclear introns (CHD2 and ZNF618). Our mitochondrial data revealed a significant divergence within the “Gazelle bennettii group,” identifying two lineages: the Iranian lineage (jebeer) and the Indian lineage (chinkara). Estimates of divergence time suggest that the split between jebeer and chinkara occurred approximately 0.895 million years ago, possibly associated with a vicariant event caused by the Indus River. These findings have important implications for assessing species conservation statuses on the IUCN Red List because an endangered lineage (jebeer) is currently grouped together with a non‐threatened one (chinkara) under the same global assessment, which underestimates the true endangered status of jebeer. In Iran, the haplotype distribution map of the jebeer can serve as a fundamental genetic framework guiding conservation efforts across Iranian protected areas and captive breeding centers. Therefore, we recommend any future management plan should be based on these findings and treat these two lineages separately.
Influence of Ocean Conditions on Heat and Mass Transfer Performance of Heat Exchangers: A Review
Authors: Jiang Haitao , Jiang Da , Wang Liang , Hua Dan 
Published: February 2025

ABSTRACTThe research reviews the study on the thermo‐mass transfer performance of heat exchangers under sloshing conditions in marine environments, aiming to promote the efficient utilization of new energy sources. It discusses the progress in the study of heat exchangers under different sloshing conditions, including theoretical models and comparisons of various swaying conditions, and comprehensively reviews the key factors influencing the enhancement or deterioration of heat transfer in heat exchangers. The research indicates that increasing the swing amplitude of spiral tube heat exchangers, reducing the swaying period, and other methods can enhance heat transfer; conversely, increasing the rolling amplitude of coil heat exchangers, extending the pitch period, and similar actions can weaken heat transfer. The mass flow rate of the working medium, steam quality, and the parameters of the sloshing motion affect the uniformity of medium distribution. When the sloshing factor is introduced, the correlation of heat transfer is highly related to the range of dimensionless numbers (such as Re and Pr) and the physical dimensions of the heat exchanger. Additionally, the article discusses techniques to mitigate the negative effects of swaying and improve heat transfer performance through improved distribution methods. Overall, the article provides significant insights into understanding the thermo‐mass transfer performance of heat exchangers in marine environments, offering valuable references for engineers and researchers in related fields.
How Sustainable Is Türkiye's Food Import? A Linearized Almost Ideal Demand System Estimation for Food Import Elasticities
Authors: Turkmen‐Ceylan Fethiye B. , Ertugrul Hasan Murat , Baycan İsmail Onur , Ulucan Hakan 
Published: February 2025

ABSTRACTThis study examines the import demand for four key agricultural commodities—cereals, meat, sugar, and vegetable oils—in Türkiye, using a Linear Almost Ideal Demand System (AIDS) model. Spanning the period from 1986 to 2020, the analysis focuses on these commodities as they constitute over 90% of Türkiye's food import budget. The results reveal significant long‐run own‐price elasticities, with vegetable oils and cereals being particularly sensitive to price changes, whereas the impact of price on meat and sugar imports is negligible. The study also highlights the limited role of income and exchange rates on import demand, except for sugar where the exchange rate has a significant but small positive effect. Short‐run estimates indicate a heightened responsiveness of budget allocations for cereals and vegetable oils to price fluctuations, suggesting a persistent element in food import patterns over time. The findings underscore the essential nature of these commodities, with low own‐price elasticity for cereals and vegetable oils, reflecting their status as necessities. In contrast, short‐run elasticity results suggest that cereal imports may be viewed as a luxury, with the potential for domestic production to substitute imports. The study concludes that Türkiye's food security is increasingly vulnerable to global price fluctuations, particularly for vegetable oils, and calls for policies that stabilize exchange rates and inflation while enhancing domestic agricultural productivity to mitigate this risk.
Molecular Mechanisms and Therapeutic Implications of Long Non‐coding RNAs in Cutaneous Biology and Disease
Authors: Zuccotti Alessandro , Al‐Fatyan Farah , Ferretti Giulia D. S. , Bertolini Irene , Long David T. , Sahin Ozgur , Rodriguez‐Blanco Jezabel , Barnoud Thibaut 
Published: February 2025

ABSTRACTHuman skin is the largest organ of the human body and accounts for approximately fifteen percent of the total bodyweight. Its main physiological role is to protect the body against a wide range of environmental factors including pathogens, ultraviolet light, and injury. Importantly, the skin can regenerate and heal upon injury in large part by the differentiation of keratinocytes. Not surprisingly, dysregulation of cutaneous differentiation and self‐renewal can result in a variety of skin‐related pathologies, including autoimmune disease and cancer. Increasing evidence supports the premise that long non‐coding RNAs (lncRNAs) act as critical mediators of gene expression and regulate important biological processes within the skin. Notably, dysregulation of lncRNAs has been shown to influence diverse physiological and pathological consequences. More recently, numerous reports have revealed new mechanistic insight on the role that lncRNAs play in skin homeostasis as well as their contribution to the pathogenesis of skin‐related disorders. Here, we review the biological functions of cutaneous lncRNAs and their impact on skin homeostasis. We also describe the fundamental roles of lncRNAs in the pathogenesis of skin‐related disorders, including fibrotic, autoimmune, and malignant diseases. Lastly, we will highlight how a better understanding of lncRNAs at the molecular level may reveal novel therapeutic approaches for the improvement of cutaneous disorders.
Single‐Step Isolation of Phyllacanthone From Cnidoscolus quercifolius Using Centrifugal Partition Chromatography
Authors: Nai Sovannychloé , Ferraz Christiane Adrielly Alves , Gaslonde Thomas , Almeida Jackson Roberto Guedes da Silva , Filho Edilson Beserra de Alencar , Leal Cíntia Emi Yanaguibashi , Musnier Benjamin , Picot Laurent , Bodet Pierre‐Edouard , Lallemand Marie‐Christine , Grougnet Raphaël , de Oliveira Junior Raimundo Gonçalves 
Published: February 2025

ABSTRACTFavelines, benzocycloheptene diterpenes exclusively found in Cnidoscolus quercifolius, have shown promising anticancer properties, particularly phyllacanthone. Nevertheless, the isolation of these compounds remains challenging due to their similar polarity. The use of centrifugal partition chromatography (CPC) has enabled the quick and efficient purification of phyllacanthone from C. quercifolius crude extract with a yield of 4.5% (w/w) and a purity of 83%. Favelines‐enriched fractions were also obtained and purified by preparative HPLC, resulting in the isolation of faveline, isofavelol, and deoxofaveline. In addition, phyllacanthone B, a previously unreported derivative, was identified and characterized. Pharmacological evaluations revealed the significant anti‐melanoma activity of purified metabolites, with distinct structure–activity relationships. These results shed light on the mechanism of action of favelines, suggesting their potential as hits for the development of novel anticancer agents.
Carbon dioxide–induced acidification enhances short‐lived brominated hydrocarbons production in oligotrophic oceans
Authors: Zou Ya‐Wen , Li Cheng‐Xuan , Wei Qin‐Sheng , Ma Qian‐Yao , Ding Hui , Duan Xiao Meng , Zhai Xing , Wang Bao‐Dong 
Published: February 2025

AbstractOceanic emission is a primary source of brominated very short‐lived substances (BrVSLs) to the atmosphere, which have important effects on stratospheric ozone chemistry. Marine biogeochemical processes regulating BrVSLs are often sensitive to ocean acidification. Yet, the response of BrVSLs production to acidification remains poorly understood. Herein, the effects of acidification on the production of two main BrVSLs, dibromomethane (CH2Br2) and tribromomethane (CHBr3), were studied by ship‐based incubation experiments at three stations in the South Atlantic and Indian Oceans. The average CH2Br2 and CHBr3 concentrations increased by 17.2–58.7% and 14.3–80.3% due to acidification under the in situ nutrient conditions with nutrient and/or iron limitation at the three stations, but the mechanisms driving these increases varied among different regions. The increased bromoperoxidase (BrPO) activity caused by acidification facilitated BrVSLs release in the Eastern Tropical Indian Ocean, where diatoms were dominant. CHBr3 increased due to acidification as a result of enhanced reactivity of dissolved organic matter (DOM) in the Eastern Tropical Atlantic, where dinoflagellates were dominant. Brominated very short‐lived substances increased due to acidification as a result of a combined effect of the above two mechanisms in the Benguela Current Coastal with high phytoplankton abundance. Under the nutrient and/or iron addition conditions with nutrient and iron sufficiency, however, acidification did not promote BrVSLs production due to its only minor effect on the BrPO activity and reactivity of DOM, partly because the effect of increased oxidative stress was offset by that of changed phytoplankton composition. Our study provided a basis for future modeling on the impact of acidification on global BrVSLs emissions.
SHCBP1 is a novel regulator of PLK1 phosphorylation and promotes prostate cancer bone metastasis
Authors: Tang Chen , Peng Shengmeng , Chen Yongming , Cheng Bisheng , Li Shurui , Zhou Jie , Wu Yongxin , Li Lingfeng , Zhong Haitao , Guo Zhenghui , Lai Yiming , Huang Hai 
Published: February 2025

AbstractProstate cancer is a common male genitourinary malignancy with bone metastasis posing challenges for prognosis and treatment. This study aimed to investigate the role of SHC protein SH2 structural domain binding protein 1 (SHCBP1) in prostate cancer bone metastasis. Whole transcriptome sequencing of prostate cancer samples was conducted to identify oncogene expression, specifically focusing on SHCBP1. In vivo and in vitro models were used to study SHCBP1's impact on bone metastasis. Through co‐immunoprecipitation, mass spectrometry, and Western blot assays, the interaction between SHCBP1 and cell cycle‐related proteins was elucidated, along with analysis of downstream protein partners. SHCBP1 was found to enhance prostate cancer cell development, metastasis, and mitosis, with the SHCBP1—polo‐like kinase 1 (PLK1)—CDC25C axis playing a key role in promoting tumorigenesis. Therapeutic inhibition of SHCBP1 increased docetaxel sensitivity. Clinical data showed elevated SHCBP1 expression in advanced prostate cancer stages. These findings offer insights into potential therapeutic strategies for prostate cancer bone metastasis and highlight the significance of the SHCBP1‐PLK1‐CDC25C axis in docetaxel sensitivity.
Development of a Telerobotic Target‐Specific Pesticide Applicator: An Intervention for Enhanced Safety and Efficiency
Authors: Naik Mude Arjun , Kumar Adarsh , Kushwaha Dilip Kumar , Kushwaha H. L. , Gupta Suman , Ramasubramanian V. , Singh Awani Kumar , Hasan Murtaza 
Published: February 2025

ABSTRACTAdvances in computing and electronics are finding applications in agriculture. These facilitate the automation of farm operations performed manually under greenhouse structures. During chemical application, farm workers are exposed to toxic chemicals and experience fatigue and drudgery with traditional spraying equipment. The traditional application methods apply chemicals uniformly, without considering the spatial variability of the canopy, which adversely impacts the economy and environment. To mitigate these, a telerobotic target‐specific pesticide applicator (robot) was designed and developed for real‐time application of pesticides based on the presence and height of foliage. It consists of a prime mover, an ultrasonic sensor‐based target‐specific pesticide application system with electronic control for navigation and spraying systems. The robot is operated from outside the greenhouse by an operator sitting in a safe environment through real‐time video. The percentage reduction in pesticide use during greenhouse field evaluation was 24.95% with activation compared to without the activation of sensors. The theoretical field capacity, effective field capacity, and field efficiency of the robot are 0.20 ha h−1, 0.15 ha h−1, and 75% respectively. The maximum range of wireless communication between the operator and the robot was 121 m. The root mean square error of deviation from a straight‐line path was 55 mm. In the context of user interface design for human–robot interaction, two display devices (a dashboard display and a first‐person view head‐mounted display [HMD]) were evaluated, along with peripheral vision (single view and adjustable multiple views). Results showed that adjustable multiple views with the HMD demonstrated statistically significant improvements in effectiveness, efficiency, and system usability scores compared with single view and dashboard display. The potential dermal exposures of the operators with a rechargeable knapsack sprayer (RKP) and with a developed robot during spraying activity were 53.75 ± 0.99 mL h−1 and zero, respectively. This means the developed robot eliminates exposure to the operator. The ergonomic parameters—heart rate, body part discomfort score, and overall discomfort score—using an RKP are 104.1 ± 6 beats min−1, 27.3 ± 2.0, and 3.5 ± 0.2, respectively, while with the developed robot, they were 99 ± 4 beats min−1, 12.1 ± 1.1, and 1.0 ± 0.5, respectively. The operating cost of the developed robot was 1.74 times higher than that of the RKP, but it offers a more precise pesticide application, reducing chemical wastage and environmental impact. The robot completes spraying on a 1500 m² greenhouse in 0.75 h compared with 4 h with a knapsack sprayer, reducing spraying time by 5.3 times and enhancing efficiency, cost savings, and operator well‐being.
High‐Performance Stable Hybrid Inorganic‐Organic Light‐Emitting Transistor
Authors: Huang Huixue , Miao Zhagen , Gao Haikuo , Cao Jin , Zheng Yanqiong , Gao Can , Li Xifeng , Yuan Guangcai , Dong Huanli 
Published: February 2025

ABSTRACTLight‐emitting transistors (LETs) as novel integrated optoelectronic devices demonstrate great potential applications in smart displays and visual intelligent perception. The construction of high‐performance area‐emission LETs with low power consumption and good reliability is urgently needed for advancing their applications, however, this integration has not been realized within a single device. Herein, we demonstrate a kind of planar‐driven hybrid LET (PDHLET) that makes use of the unique advantages of high mobility and stability of inorganic and organic semiconductors in the same device. By incorporating an indium‐zinc‐gallium‐oxide (InZnGeO) conducting layer and organic emissive layer, a high‐performance stable blue‐emissive PDHLET is constructed, giving a high Ion/Ioff ratio approaching 6.1 × 108 and a low Von of 5.5 V along with maximum brightness of 1264 cd/m2 as well as small VTH shift of 0.5 V after 1000 s positive stress bias. Finally, a systematic simulation, including charge concentration and Langevin recombination rate, is carried out on PDHLET for the first time, demonstrating good consistency with experimental results. This confirms the uniformity of high redistributed charge concentration in the InZnGeO conducting layer which thus enables good area emission. This study provides a new avenue for constructing high‐performance stable LETs to advance various field applications.
Numerical simulation study on the mechanical behaviors of frost‐damaged recycled sand powder concrete
Authors: Ma Quan , Xiao Jianzhuang , Yang Wei , Duan Zhenhua , Tian Feng 
Published: February 2025

AbstractThis study established a multiphase, three‐dimensional finite element model of recycled sand powder concrete (RSPC) using the ABAQUS software. A freeze–thaw damage model based on the deterioration of mechanical properties was developed from experimental freeze–thaw test results and applied to the micro‐scale numerical simulation. The results indicated that the mechanical properties of RSPC deteriorate with increasing freeze–thaw cycles, and defects in recycled micro‐powder and recycled fine aggregates become more pronounced during the freeze–thaw process. The numerical simulation results showed a maximum error of 12.97% compared to experimental data (all within 15%), confirming the validity of the model. Specimen failure was primarily concentrated at the bonding interface between aggregates and new/old mortar, with an overall “X‐shaped” failure pattern. Furthermore, as the number of freeze–thaw cycles increased, defects in ITZ2 (the interfacial transition zone between aggregates and old mortar) accelerated the propagation of freeze–thaw damage within the concrete, significantly amplifying internal damage in RSPC.
Zusammensetzung von Schockraumteams
Authors: Pedersen Vera , Waydhas Christian , Clemens Valentin , Özkurtul Orkun , Hackenberg Lisa , Pfläging Tristan , Lefering Rolf , Nohl André , Schweigkofler Uwe , Fröhlich Matthias , Laue Fabian , Baacke Markus , Störmann Philipp , Düsing Helena , Sprengel Kai , Paffrath Thomas , Jensen Kai Oliver , Faul Philipp , Ahnert Tobias , Imach Sebastian , Kleber Christian , Keß Anette , Bieler Dan , Trentzsch Heiko ,   Studiengruppe NIS Trauma-Team Activation and Trauma/Injury Care (NIS-TAcTIC), Becker Lars , Flohe Sascha , Huber-Wagner Stefan , Kamp Oliver , Laumann Marion , Mand Carsten , Matthes Gerrit , Wagner Frithjof , Wohlrath Bernd 
Published: February 2025

Zusammenfassung

Hintergrund
Die Bereitstellung spezialisierter Schockraumteams zur Schwerverletztenversorgung ist nach den Vorgaben der S3-Leitlinie Polytrauma/Schwerverletztenversorgung der AMWF obligat und die Zusammensetzung durch das Weißbuch Schwerverletztenversorgung festgelegt. In jeder Versorgungsstufe wird das Basisteam aus den 4 Disziplinen Orthopädie und Unfallchirurgie, Anästhesie, Radiologie und der Notfallpflege der Notaufnahme zusammengesetzt, mit weiteren Anpassungen je nach Versorgungsstufe des Krankenhauses. Ziel der vorliegenden Studie ist die Untersuchung der gelebten Realität bei der Zusammensetzung der Schockraumteams.

Methodik
Bei der prospektiven, multizentrischen Beobachtungsstudie wurden in 12 überregionalen Traumazentren in Deutschland und der Schweiz insgesamt 3753 Patienten nach Unfällen in der Notaufnahme behandelt, darunter 964 Patienten (26 %) nach vorangegangener Schockraumalarmierung.

Ergebnisse
In 94,7 % der Schockraumversorgungen waren alle 4 der geforderten Disziplinen anwesend; im Durchschnitt waren 6 Personen an der Schockraumversorgung beteiligt. Die 48-h-Mortalität betrug 3 % der über den Schockraum versorgten Patienten; bei allen verstorbenen Patienten waren während der Schockraumversorgung alle 4 Disziplinen anwesend. Bei Patienten mit mindestens einem Alarmierungskriterium der Kategorie A waren bei 97,7 % der Versorgung ein vollständiges Team aus 4 Disziplinen an der Versorgung beteiligt.

Diskussion
In fast 98 % der Fälle, in denen Alarmierungskriterien der Kategorie A vorliegen, sind alle 4 der im Weißbuch geforderten Disziplinen zur Patientenversorgung im Schockraum anwesend. Dies geht mit einer mittleren Ressourcenbindung von 6,6 Personen einher. Das Fehlen einer oder mehrerer Disziplinen bei der Schockraumversorgung scheint die frühe Mortalität der Schwerverletzten nicht signifikant zu beeinflussen.

Graphic abstract