bluuebunny/crossref_metadata_2025_split huggingface dataset:
Influence of anti‐inflammatory administration in collagen maturation process during orthodontic tooth movement
Authors: Retamoso Luciana , Knop Luegya , Shintcovsk Ricardo , Maciel José Vinícius , Machado Maria Angela , Tanaka Orlando 
Published: December 2010

AbstractBone formation is essential to orthodontic tooth movement and bone is formed by collagen. To analyze the collagen maturation process on bone matrix neoformed under nonsteroidal and steroidal treatment during orthodontic tooth movement by polarized microscopy, male Wistar rats (n = 90) were randomly divided into three groups (n = 30): C (control), NSAID (potassium diclofenac) and SAID (disodic phosphate dexamethasone). The animals of the C group received 0.9% saline solution; NSAID group received 5 mg/kg potassium diclofenac (CATAFLAM®); and SAID group received 2 mg/kg phosphate dissodic dexamethasone (DEXANIL®). Animals were sacrificed 3, 7 or 14 days after the placement of orthodontic appliances and the upper first molars were processed histologically and stained with picrosirius. Bone formation was evaluated under polarized light microscopy and 4.5 Image Pro‐Plus® software calculated the percentage of immature/mature collagen present in the groups. On the third days after force application, SAID and NSAID groups showed greater proportion of immature collagen than C group. On the seventh and fourteenth days, there was a lower proportion of mature collagen only in the SAID group (P < 0.001). These data demonstrate that dexamethasone delays the collagen maturation process in established bone matrix. Microsc. Res. Tech., 2011. © 2010 Wiley‐Liss, Inc.
Single molecule microscopy methods for the study of DNA origami structures
Authors: Birkedal Victoria , Dong Mingdong , Golas Monika M. , Sander Bjoern , Andersen Ebbe Sloth , Gothelf Kurt Vesterager , Besenbacher Flemming , Kjems Jørgen 
Published: December 2010

AbstractSingle molecule microscopy techniques play an important role in the investigation of advanced DNA structures such as those created by the DNA origami method. Three single molecule microscopy techniques are particularly interesting for the investigation of complex self‐assembled three‐dimensional (3D) DNA nanostructures, namely single molecule fluorescence microscopy, atomic force microscopy (AFM), and cryogenic transmission electron microscopy (cryo‐EM). Here we discuss the strengths of these three techniques and demonstrate how their interplay can yield very important and unique new insights into the structure and conformation of advanced biological nanostructures. The applications of the three single molecule microscopy techniques are illustrated by focusing on a self‐assembled DNA origami 3D box nanostructure. Its size and structure were studied by AFM and cryo‐EM, while the lid opening, which can be controlled by the addition of oligonucleotide keys, was recorded by Förster/fluorescence resonance energy transfer (FRET) spectroscopy. Microsc. Res. Tech., 2011. © 2010 Wiley‐Liss, Inc.
Visualization of bionanostructures using transmission electron microscopical techniques
Authors: Sander Bjoern , Golas Monika M. 
Published: December 2010

AbstractIn the recent years, nanotechnology has rapidly evolved as promising toolbox for many applications, including sensing and drug delivery. Nanotechnology aims at forming man‐designed two‐dimensional and three‐dimensional structures in the nanometer scale using e.g., the self‐assembly properties of smaller building blocks such as DNA and RNA. The visualization and structural characterization of these nanostructures do not only provide evidence for the correct formation of the desired shapes, but can also contribute to a better understanding of their formation and functionality. Transmission electron microscopy offers the possibility to directly visualize the individual nanostructures. The vitrification of the sample by using the plunge‐freezing method and subsequent electron cryomicroscopy (cryo‐EM) provides in‐solution snapshots of the nanostructures under cryogenic conditions and thus preserves the close‐to‐native structure of the particles. Here, we describe the plunge‐freezing and other sample preparation protocols such as negative staining and cryo‐negative staining as well as the various imaging and image processing methods, including electron crystallography, electron tomography, and single‐particle EM. Typical example applications are provided together with a discussion of benefits and shortcomings of these approaches. We also discuss how deviations from an ideal symmetry and structural heterogeneity, in general, can limit the resolution. Finally, we suggest that nanotechnological approaches may not only offer new applications in the field of nanomaterial science and nanomedicine, but may also emerge as tools for structural biology and structure‐related biomedical research. Microsc. Res. Tech., 2011. © 2010 Wiley‐Liss, Inc.
Synthesis of benzo[3,4‐h][1,6]naphthyridines via Friedländer condensation with active methylenes
Authors: Rote Ramhari V. , Bagul Sandeep M. , Shelar Deepak P. , Patil Sandeep R. , Toche Raghunath B. , Jachak Madhukar N. 
Published: December 2010

AbstractNovel 4‐amino‐6‐chloroquinoline‐3‐carbaldehyde has been synthesized by synchronous reduction by lithium aluminiumhydride and finally oxidation with MnO2. Friedländer condensation of it with reactive methylenes furnished novel benzo[3,4‐h][1,6]naphthyridine derivatives. J. Heterocyclic Chem., (2011).
Characterization of the bone phenotype and fracture repair in osteopetrotic Incisors absent rats
Authors: McDonald Michelle M. , Morse Alyson , Peacock Lauren , Mikulec Kathy , Schindeler Aaron , Little David G. 
Published: December 2010

AbstractOsteopetrotic patients possess a genetic condition that leads to a deficiency in osteoclast number or function. Patients have a high bone density and suffer from an increased risk of fracture. The lack of normal osteoclast activity has the potential to impede repair by complicating orthopedic fixation and/or by affecting the biology of fracture healing. The naturally occurring incisors absent (ia/ia) rat was adopted as a rodent model of congenital osteopetrosis. A detailed phenotypic analysis of the ia/ia rat indicated that some functional recovery occurred between 7 and 9 weeks. Consequently a fracture repair study was undertaken using 5‐week‐old rats. Closed femoral fractures were generated in ia/ia rats and control ia/+ and +/+ rats using an Einhorn apparatus. Fracture healing was examined radiologically and histologically at 1–3 weeks. No difference was seen in bridging between ia/ia and control rats at any time point. The ia/ia rats showed no delay in cartilage removal but showed a significant delay in hard callus remodeling. This is consistent with an essential role for osteoclasts in only the latter stages of endochondral bone repair. This delay in hard callus remodeling was offset by an increase in moment of inertia. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 29:726–733, 2011
Analysis of testosterone in human urine using molecularly imprinted solid‐phase extraction and corona discharge ion mobility spectrometry
Authors: Mirmahdieh Shiva , Mardihallaj Azam , Hashemian Zahra , Razavizadeh Jalal , Ghaziaskar Hassan , Khayamian Taghi 
Published: December 2010

AbstractAnalysis of testosterone was accomplished using corona discharge ion mobility spectrometry. Molecular imprinted polymer was used for the extraction and pre‐concentration of testosterone. Analytical parameters including precision, dynamic range and detection limit were obtained. The linear dynamic range was from 10 to 250 ng/mL and the limit of detection was 0.9 ng/mL. The proposed method was used for analysis of testosterone in urine samples. A urine sample from a 3‐year‐old girl was used as the blank. The RSD was below 10%. The obtained results from the method were also compared with the standard method for analysis of testosterone using SPE‐HPLC analysis. The results demonstrate the accuracy of the method.
The use of GC×GC/TOF MS with multivariate analysis for the characterization of foodborne pathogen bacteria profiles
Authors: Gardner J. Yvette , Brillhart Darcy E. , Benjamin Melissa M. , Dixon Lurlene G. , Mitchell LaTonya M. , Dimandja Jean‐Marie D. 
Published: December 2010

AbstractThe cellular fatty acid profiles of eight strains of Bacillus, Staphylococcus, and Enterobacteriacae (Escherichia coli and Salmonella) were analyzed by comprehensive two‐dimensional gas chromatography/time‐of‐flight mass spectrometry. A novel template method was developed to standardize the raw two‐dimensional gas chromatography retention data through the use of a chemical indexing mixture. Analyte retention coordinates were normalized in the primary dimension with respect to a series of n‐alkanes (Kovats index) and in the secondary dimension with respect to a series of aromatic hydrocarbons (Lee index). Fatty acid profiles extracted from the templates were compared by multidimensional scaling and principal component analysis. Differences in the profiles of Gram‐positive and Gram‐negative bacteria were observed, and a series of heterogeneous mixtures comprising different fractions (containing one Gram‐positive and one Gram‐negative bacteria strain) were also distinguished from their homogeneous constituents.
Efficacy of exposure therapy for Japanese patients with posttraumatic stress disorder due to mixed traumatic events: A randomized controlled study
Authors: Asukai Nozomu , Saito Azusa , Tsuruta Nobuko , Kishimoto Junji , Nishikawa Toru 
Published: December 2010

AbstractThe authors examined the efficacy of Prolonged Exposure (PE) therapy in Japanese patients with posttraumatic stress disorder (PTSD). Twenty‐four patients (21 women, 3 men) with PTSD due to mixed trauma were randomly assigned to the PE group (PE with or without treatment as usual [TAU]) or the control group (TAU) only. The control group received PE after a 10‐week period. Intention‐to‐treat analysis showed the PE group achieved significantly greater reduction than the control group at posttreatment in either PTSD or depressive symptoms. The control group had significantly decreased symptom severity after PE treatment. Symptom levels of 19 PE completers in the both groups remained low in 12‐month follow‐up assessments. The study's findings will promote the future dissemination and implementation of evidence‐based treatment for PTSD in non‐Western settings.
The surgical management of goiter: Part I. preoperative evaluation
Authors: Shin Jennifer J. , Grillo Hermes C. , Mathisen Doug , Katlic Mark R. , Zurakowski David , Kamani Dipti , Randolph Gregory W. 
Published: December 2010

AbstractObjectives/Hypothesis:Our overarching objective is to provide a comprehensive analysis of goiter data in two paired articles. This first article focuses on the preoperative evaluation. The following null hypotheses have been tested: 1) there is no correlation between goiter size and preoperative symptoms, 2) there is no correlation between preoperative neck imaging abnormalities and preoperative symptoms, and 3) there are no predictors for goiter recurrence.Sudy Design:A retrospective review of 200 consecutive thyroidectomies meeting inclusion/exclusion criteria for cervical or substernal goiter.Results:The mean specimen size was 10.5 ± 4.8 cm and 142.9 ± 113.3 grams. There was a positive correlation between goiter size and preoperative shortness of breath (P = .02). The presence of substernal goiter was significantly correlated with tracheal deviation and tracheal compression (P < .01) on neck imaging. There was strong correlation between preoperative shortness of breath and tracheal compression (P < .001) on neck computed tomography (CT), but not tracheal deviation. The need for revision surgery was significantly associated with female gender (odds ratio 3.0; 95% confidence interval [CI] 1.5, 6.1, and a positive family history of thyroid disease (odds ratio 6.5 [2.4, 17.3]).Conclusions:Goiter size is associated with increasing symptoms. Tracheal compression but not deviation was related to shortness of breath. Females and patients with a positive family history of goiter have an increased risk of goiter recurrence. Laryngoscope, 2011
Severe rhabdomyolysis due to rosuvastatin in a liver transplant subject with human immunodeficiency virus and immunosuppressive therapy-related dyslipidemia
Authors: Moreno Ana , Fortún Jesús , Graus Javier , Rodriguez-Gandía Miguel A. , Quereda Carmen , Pérez-Elías María J. , Nuño Javier , Wikman Philip , Moreno Santiago , Bárcena Rafael 
Published: December 2010

Abstract

Statins are relatively safe first-line agents to use in the setting of dyslipidemia associated with immunosuppressive therapy in subjects undergoing liver transplantation, and also in HIV-infected patients with dyslipidemia due to antiretroviral drugs, especially ritonavir-boosted protease inhibitors. Rosuvastatin, a new statin, has demonstrated higher potency than previously released statins and is not extensively metabolized by the liver P450 system; therefore, the probability of deleterious pharmacokinetic interactions with commonly used immunosuppressants and antiretroviral drugs is reduced. We present the first case of severe rhabdomyolysis in a liver transplant patient receiving rosuvastatin for the treatment of immunosuppressive therapy-related grade IV dyslipidemia, an HIV-infected subject on protease inhibitor-sparing HAART, that resolved after rosuvastatin withdrawal, probably related to interactions between calcineurin inhibitors and hepatic rosuvastatin uptake transporters such as Organic Anion Transporting Polypeptides (OATPs). Liver Transpl, 2011. © 2011 AASLD.
Posttransplantation prophylaxis with primary high-dose hepatitis B immunoglobulin monotherapy and complementary preemptive antiviral add-on a
Authors: Hwang Shin , Ahn Chul-Soo , Song Gi-Won , Kim Ki-Hun , Moon Deok-Bog , Oh Heung-Bum , Lim Young-Suk , Lee Han Chu , Ha Tae-Yong , Jung Dong-Hwan , Chung Young-Hwa , Lee Sung-Gyu 
Published: December 2010

Abstract
A considerable proportion of liver transplantation recipients who receive hepatitis B immunoglobulin (HBIG) monotherapy for hepatitis B virus (HBV) prophylaxis develop resistance to HBIG. We retrospectively assessed the efficacy of HBV prophylaxis in 1524 patients who received primary high-dose HBIG monotherapy (n = 1463) or with a preemptive antiviral add-on as secondary combination therapy (n = 61). At a median follow-up time of 57 months, 106 (7.3%) patients receiving HBIG monotherapy experienced HBV recurrence, with a 10-year HBV recurrence rate of 9.8%, compared to none of the patients receiving preemptive combination therapy (P = 0.047). Thirteen patients (12.3%) with HBV recurrence failed antiviral therapy, leading to death or retransplantation. Response rates to rescue therapy before and after use of adefovir/entecavir were 44.4% and 91.8%, respectively. Acute exacerbation was not associated with treatment failure, but required prolonged treatment. Of 84 surviving patients with HBV recurrence, 44 (52.4%) showed no evidence of blood HBV DNA. The Gly145Arg mutation was found in 11 of 15 (73.3%) patients, whereas 25 of 71 (35.2%), 2 of 29 (6.9%), and 4 of 8 (50%) patients were resistant to lamivudine, adefovir, and entecavir, respectively. In conclusion, our finding of a 10-year HBV recurrence rate of 9.8% in patients receiving high-dose HBIG monotherapy indicates that this treatment is effective but requires complementary measures. Strict surveillance following HBIG monotherapy is necessary to enhance responses to rescue antiviral therapy. Preemptive conversion to combination therapy has a complementary role in prophylaxis with primary high-dose HBIG monotherapy, especially for patients at high risk of HBV recurrence.
Liver Transpl, 2011. © 2011 AASLD.
Pretransplant depression, antidepressant use, and outcomes of orthotopic liver transplantation
Authors: Rogal Shari S. , Landsittel Douglas , Surman Owen , Chung Raymond T. , Rutherford Anna 
Published: December 2010

Abstract

Depression is a common problem among patients awaiting organ transplantation, but little is known about the impact of depression and its treatment on the outcomes of liver transplantation. In this retrospective cohort analysis, we studied all patients over 18 years of age who underwent liver transplantation during a 5-year period (2004-2008) at a single center. Among 179 recipients, 65 patients had depression, as defined by a health care provider assessment, before transplantation. Depression was defined as past or active depression or an adjustment disorder. The associations between pretransplant depression and various outcomes (time to death, graft failure, first acute cellular rejection episode, first infection, and first rehospitalization) were assessed. In the entire sample, more patients with depression required posttransplant psychiatric care (37% versus 18%); the adjusted hazard ratio was 2.28 (1.27-4.11). The rates of other outcomes, including hospital readmission, acute cellular rejection, graft failure, mortality, and infection, were similar for patients with depression and patients without depression. Among those with depression, patients on antidepressants at the time of transplantation had acute cellular rejection less frequently than those not taking antidepressants (13% versus 40%); the adjusted hazard ratio was 0.14 (0.03-0.62). The rates of other outcomes were similar between these 2 groups. These data indicate that depression affects posttransplant psychiatric morbidity but not other medical outcomes of liver transplantation. Pharmacological treatment of depression may significantly reduce the incidence of acute cellular rejection in patients undergoing liver transplantation. However, future prospective studies of mental health and liver transplantation are required to definitively assess the effects of antidepressant medications on medical outcomes. Liver Transpl, 2011. © 2011 AASLD.
Liver allograft antibody-mediated rejection with demonstration of sinusoidal C4d staining and circulating donor-specific antibodies
Authors: Kozlowski Tomasz , Rubinas Tara , Nickeleit Volker , Woosley John , Schmitz John , Collins Dana , Hayashi Paul , Passannante Anthony , Andreoni Kenneth 
Published: December 2010

Abstract
The importance of antibody-mediated rejection (AMR) in ABO-compatible liver transplantation is controversial. Here we report a prospective series of liver recipients with a preoperative positive crossmatch. To establish the diagnosis of AMR in liver recipients, the criteria described for kidney allografts were adopted. In approximately 10% of 197 liver transplants, we observed a positive T and B cell flow crossmatch before transplantation. Fifteen of 19 patients converted to negative crossmatches early after transplantation and displayed normal liver function while they were on routine immunosuppression. Four patients maintained positive crossmatches. Three of the 4 met the criteria for AMR and showed evidence of graft dysfunction, the presence of donor-specific antibodies (DSAs), morphological tissue destruction with positive C4d linear staining on the graft sinusoidal endothelium, and improved function with attempts to eliminate DSAs. A persistently positive crossmatch after liver transplantation may lead to early, severe AMR and liver failure. C4d staining in the liver sinusoidal endothelium should alert one to the possibility of AMR. In our experience, patients with a positive crossmatch should have it repeated at 2 weeks and, if it is positive, again at 3 to 5 weeks. Recipients with an unknown preoperative crossmatch who develop early cholestasis of unclear etiology should be crossmatched or tested for the presence of DSAs to evaluate for AMR.
Liver Transpl, 2011. © 2011 AASLD.
Clinical value of myocardial perfusion scintigraphy as a screening tool in liver transplant candidates
Authors: Oprea-Lager Daniela E. , Sorgdrager Bastiaan J. , Jukema Wouter J. , Scherptong Roderick W. C. , Ringers Jan , Coenraad Minneke J. , van Hoek Bart , Stokkel Marcel P. M. 
Published: December 2010

Abstract

A cardiac evaluation before orthotopic liver transplantation (OLT) is imperative. Previous investigations have demonstrated that mild to moderate reversible perfusion defects on myocardial perfusion scintigraphy (MPS) in general are associated with a low risk for perioperative cardiac events. The objective of this study was to assess any perfusion defects in consecutive patients with chronic liver disease who were undergoing OLT. OLT candidates underwent extensive cardiovascular screening that included, among other methods, MPS. Patients who had no contraindications for surgery and underwent OLT were followed up. The occurrence and risk of complications and mortality were compared in 3 groups of patients: patients with normal MPS results, patients with any reversible defect, and patients with a fixed perfusion defect on MPS. In all, 156 subsequent patients underwent OLT. One or more reversible segmental perfusion defects on MPS were present in 14 patients (<3 segments, n = 12; 3 segments without obstructive coronary artery disease, n = 2). The risk of complications did not differ significantly between patients with normal MPS findings and patients with a reversible perfusion defect (odds ratio = 3.04, 95% confidence interval = 0.65-14.26, P = 0.16), although the study was not sufficiently powered to show a difference. The presence of 1 or more reversible defects on MPS was significantly associated with an increased incidence of all-cause 1-year mortality (hazard ratio = 3.17, 95% confidence interval = 1.02-9.83, P = 0.046). No significant difference in the outcomes of patients with normal MPS findings and patients with a fixed defect on MPS was found; the study was, however, not adequately powered to do so. In conclusion, the results of this small cohort study indicate that patients with mild to moderate reversible perfusion defects on MPS may have inferior survival characteristics in comparison with patients with normal MPS results. A prospective, adequately powered study is required to confirm the results of this study. Liver Transpl, 2011. © 2011 AASLD.
My earliest experiences in leadership
Authors: Bennis Warren , Ward Biederman Patricia 
Published: December 2010

AbstractIn this excerpt from his latest book, Still Surprised: A Memoir of a Life in Leadership, Bennis writes about his experiences as a 19‐year‐old infantry officer during the Battle of the Bulge. Looking back more than 65 years, he sees leadership lessons he was not aware he was learning at the time, such as the central importance role plays in the development of leaders.
Intelligence multipliers: How smart leaders double the power of their workforce for free
Authors: Wiseman Liz 
Published: December 2010

AbstractOrganizations of all types face ongoing constraints—and leaders continue to exhort employees to “do more with less.” Yet many of these same leaders, Liz Wiseman says, “can't see or use the full capabilities of the people around them.” These leaders are Diminishers, Wiseman says, “leaders whose behavior causes employees to use only half of their capabilities.” She explains how leaders can accidentally diminish the human resources available to them and shows how smart leaders are actually able to double the brainpower of the employees in their organizations.