bluuebunny/crossref_metadata_2025_split huggingface dataset:
Cells with Characteristics of Cancer Stem/Progenitor Cells Express the CD133 Antigen in Human Endometrial Tumors
Authors: Rutella Sergio , Bonanno Giuseppina , Procoli Annabella , Mariotti Andrea , Corallo Maria , Prisco Maria Grazia , Eramo Adriana , Napoletano Chiara , Gallo Daniela , Perillo Alessandro , Nuti Marianna , Pierelli Luca , Testa Ugo , Scambia Giovanni , Ferrandina Gabriella 
Published: June 2009

Abstract
Purpose: Cancer stem cells represent an attractive therapeutic target for tumor eradication. The present study aimed to determine whether CD133 expression may identify cells with characteristics of cancer stem/progenitor cells in human endometrial tumors.
Experimental Design: We analyzed 113 tumor samples for CD133/1 expression by flow cytometry, immunohistochemistry, and semiquantitative reverse transcription–PCR. CD133+ cells were isolated and used to assess phenotypic characteristics, self-renewal capacity, ability to maintain CD133 expression and form sphere-like structures in long-term cultures, sensitivity to chemotherapeutic agents, gene expression profile, and ability to initiate tumors in NOD/SCID mice.
Results: Primary tumor samples exhibited a variable degree of immunoreactivity for CD133/1, ranging from 1.3% to 62.6%, but stained negatively for other endothelial and stem cell–associated markers. Isolated CD133+ cells expanded up to 4.6-fold in serum-replenished cultures and coexpressed the GalNAcα1-O-Ser/Thr MUC-1 glycoform, a well-characterized tumor-associated antigen. Dissociated bulk tumors formed sphere-like structures; cells grown as tumor spheres maintained CD133 expression and could be propagated for up to 12 weeks. CD133+ cells purified from endometrioid adenocarcinomas were resistant to cisplatin-induced and paclitaxel-induced cytotoxicity and expressed a peculiar gene signature consisting of high levels of matrix metalloproteases, interleukin-8, CD44, and CXCR4. When serially transplanted into NOD/SCID mice, CD133+ cells were capable of initiating tumor formation and recapitulating the phenotype of the original tumor.
Conclusions: CD133 is expressed by human endometrial cancers and might represent a valuable tool to identify cells with cancer stem cell characteristics.
Copper Transporter 2 Regulates the Cellular Accumulation and Cytotoxicity of Cisplatin and Carboplatin
Authors: Blair Brian G. , Larson Christopher A. , Safaei Roohangiz , Howell Stephen B. 
Published: June 2009

Abstract
Purpose: Copper transporter 2 (CTR2) is known to mediate the uptake of Cu+1 by mammalian cells. Several other Cu transporters, including the influx transporter CTR1 and the two efflux transporters ATP7A and ATP7B, also regulate sensitivity to the platinum-containing drugs. We sought to determine the effect of CTR2 on influx, intracellular trafficking, and efflux of cisplatin and carboplatin.
Experimental Design: The role of CTR2 was examined by knocking down CTR2 expression in an isogenic pair of mouse embryo fibroblasts consisting of a CTR1+/+ line and a CTR1−/− line in which both CTR1 alleles had been deleted. CTR2 levels were determined by quantitative reverse transcription-PCR and Western blot analysis. Cisplatin (DDP) was quantified by inductively coupled plasma mass spectrometry and 64Cu and [14C]carboplatin (CBDCA) accumulation by γ and scintillation counting.
Results: Deletion of CTR1 reduced the uptake of Cu, DDP, and CBDCA and increased resistance to their cytotoxic effects by 2- to 3-fold. Knockdown of CTR2 increased uptake of Cu only in the CTR1+/+ cells. In contrast, knockdown of CTR2 increased whole-cell DDP uptake and DNA platination in both CTR1+/+ and CTR1−/− cells and proportionately enhanced cytotoxicity while producing no effect on vesicular accumulation or efflux. A significant correlation was found between CTR2 mRNA and protein levels and sensitivity to DDP in a panel of six ovarian carcinoma cell lines.
Conclusions: CTR2 is a major determinant of sensitivity to the cytotoxic effects of DDP and CBDCA. CTR2 functions by limiting drug accumulation, and its expression correlates with the sensitivity of human ovarian carcinoma cell lines to DDP.
Tissue Targeting in Cancer: eIF4E's Tale
Authors: Borden Katherine L.B. 
Published: June 2009

Abstract
The eukaryotic translation initiation factor eIF4E is elevated in many human cancers. Tissue-specific targeting of eIF4E activity in ovarian cancer cells is achieved in cell culture and in mice by fusing a peptide corresponding to the eIF4E inhibitor, the eIF4E binding protein 1 (BP1), to an agonist of the gonadotropin receptor.
Photodynamic Therapy: A Light in the Darkness?
Authors: Lin Alexander , Hahn Stephen M. 
Published: June 2009

Abstract
Photodynamic therapy (PDT) is used to treat localized premalignant or early malignant disease. This study reports the enhanced, systemic antitumor immune response from PDT, suggesting that it may have a potential role as a therapy to address both local and disseminated disease.
Epigenetics in cancer: Targeting chromatin modifications
Authors: Ellis Leigh , Atadja Peter W. , Johnstone Ricky W. 
Published: June 2009

Abstract
Posttranslational modifications to histones affect chromatin structure and function resulting in altered gene expression and changes in cell behavior. Aberrant gene expression and altered epigenomic patterns are major features of cancer. Epigenetic changes including histone acetylation, histone methylation, and DNA methylation are now thought to play important roles in the onset and progression of cancer in numerous tumor types. Indeed dysregulated epigenetic modifications, especially in early neoplastic development, may be just as significant as genetic mutations in driving cancer development and growth. The reversal of aberrant epigenetic changes has therefore emerged as a potential strategy for the treatment of cancer. A number of compounds targeting enzymes that regulate histone acetylation, histone methylation, and DNA methylation have been developed as epigenetic therapies, with some demonstrating efficacy in hematological malignancies and solid tumors. This review highlights the roles of epigenetic modifications to histones and DNA in tumorigenesis and emerging epigenetic therapies being developed for the treatment of cancer. [Mol Cancer Ther 2009;8(6):1409–20]
Regulated cell death pathways: New twists in modulation of BCL2 family function
Authors: Sasi Nidhish , Hwang Misun , Jaboin Jerry , Csiki Ildiko , Lu Bo 
Published: June 2009

Abstract
A number of cell death pathways have been recognized. Though apoptosis and autophagy have been well characterized, programmed necrosis has recently received attention and may provide clinical alternatives to suppress resistant tumors. Necrosis is primarily characterized by large-scale permeabilization, swelling, and rupture of cell membranes and the release of pro-inflammatory cytokines. Traditionally, necrosis in cancer cells has been indicative of poor prognoses, as chronic inflammation was found to encourage tumor growth. Yet, many antitumor effects associated with necrosis have been discovered in certain settings, such as the formation of an effective antitumor immune response. In this way, finding ways to attenuate the pro-tumor effects of necrosis while engaging the antitumor pathways via drugs, radiation, and sensitization may prove valuable as a clinical focus for the future. We hypothesize that the use of Bcl-2 inhibitors may enhance necrotic death characterized by inflammation and antitumor immunity. In this article, we briefly review apoptosis and autophagy and reason how necrosis may be a suitable alternative therapeutic endpoint. We then highlight novel inhibitors of Bcl-2 that may provide clinical application of our hypothesis in the future. [Mol Cancer Ther 2009;8(6):1421–9]
Hypoxia prevents etoposide-induced DNA damage in cancer cells through a mechanism involving hypoxia-inducible factor 1
Authors: Sullivan Richard , Graham Charles H. 
Published: June 2009

Abstract
Intratumoral hypoxia is associated with resistance to therapy in many human cancers, and preexposure of tumor cells to hypoxia confers multidrug resistance. Whereas most anticancer drugs kill proliferating tumor cells by causing DNA damage, a role for hypoxia in the prevention and/or repair of drug-induced DNA damage has not been clear. Using the alkaline comet assay, we provide direct evidence that hypoxia-induced resistance to etoposide in human tumor cells (MDA-MB-231 breast carcinoma and DU-145 prostatic adenocarcinoma) is mainly due to prevention of drug-induced DNA damage (i.e., strand breaks) and that the amount of DNA damage present immediately after etoposide exposure is a good independent predictor of clonogenic survival. Our results also revealed that preexposure to hypoxia did not affect the apparent DNA repair capacity of cells. These findings indicate that the extent of DNA damage resulting from etoposide exposure is a more important determinant of survival than subsequent events after DNA damage. Furthermore, immunofluorescence analysis showed that, in a subpopulation of cells, preexposure to hypoxia decreased the levels of topoisomerase IIα, an enzyme that generates DNA strand breaks when poisoned with etoposide. Treatment of cells with small interfering RNA targeting hypoxia-inducible factor 1 prevented the hypoxia-induced decreases in topoisomerase IIα levels, abolished the protective effect of hypoxia against etoposide-induced DNA damage, and inhibited hypoxia-induced etoposide resistance. These findings support a model of hypoxia-induced drug resistance in which etoposide-induced DNA damage is prevented by HIF-1–dependent adaptations to hypoxia. [Mol Cancer Ther 2009;8(6):1702–13]
Silibinin inhibits translation initiation: implications for anticancer therapy
Authors: Lin Chen-Ju , Sukarieh Rami , Pelletier Jerry 
Published: June 2009

AbstractSilibinin is a nontoxic flavonoid reported to have anticancer properties. In this study, we show that silibinin exhibits antiproliferative activity on MCF-7 breast cancer cells. Exposure to silibinin leads to a concentration-dependent decrease in global protein synthesis associated with reduced levels of eukaryotic initiation factor 4F complex. Moreover, polysome profile analysis of silibinin-treated cells shows a decrease in polysome content and translation of cyclin D1 mRNA. Silibinin exerts its effects on translation initiation by inhibiting the mammalian target of rapamycin signaling pathway by acting upstream of TSC2. Our results show that silibinin blocks mammalian target of rapamycin signaling with a concomitant reduction in translation initiation, thus providing a possible molecular mechanism of how silibinin can inhibit growth of transformed cells. [Mol Cancer Ther 2009;8(6):1606–12]
Angiotensin-(1-7) inhibits tumor angiogenesis in human lung cancer xenografts with a reduction in vascular endothelial growth factor
Authors: Soto-Pantoja David R. , Menon Jyotsana , Gallagher Patricia E. , Tallant E. Ann 
Published: June 2009

Abstract
Angiotensin-(1-7) [Ang-(1-7)] is an endogenous seven-amino acid peptide hormone with antiproliferative properties. Our previous studies showed that Ang-(1-7) inhibits the growth of human lung cancer cells in vitro and reduces the size of human lung tumor xenografts in vivo. In the current study, s.c. injection of Ang-(1-7) not only caused a significant reduction in human A549 lung tumor growth but also markedly decreased vessel density, suggesting that the heptapeptide inhibits angiogenesis to reduce tumor size. A decrease in human endothelial cell tubule formation in Matrigel was observed following a 16 h incubation with Ang-(1-7), with a maximal reduction at a 10 nmol/L concentration. Ang-(1-7) had similar antiangiogenic effects in the chick chorioallantoic membrane, causing a &gt;50% decrease in neovascularization. The Ang-(1-7)-induced reduction in both endothelial cell tubule formation and vessel formation in the chick was completely blocked by the specific Ang-(1-7) receptor antagonist [d-proline7]-Ang-(1-7), suggesting that these biological actions are mediated by an AT(1-7) receptor. Ang-(1-7) significantly reduced vascular endothelial growth factor-A protein and mRNA in tumors from mice treated with the heptapeptide compared with saline controls as well as in the parent A549 human lung cancer cells in culture. These results suggest that Ang-(1-7) may attenuate tumor angiogenesis by reducing vascular endothelial growth factor-A, a primary proangiogenic protein. Taken together, this study shows that Ang-(1-7) exhibits significant antiangiogenic activity and may be a novel therapeutic agent for lung cancer treatment targeting a specific AT(1-7) receptor. [Mol Cancer Ther 2009;8(6):1676–83]
Babes in the Woods: Wilderness Aesthetics in Children's Stories and Toys, 1830-1915
Authors: Varga Donna 
Published: June 2009

AbstractRepresentations of nonhuman wild animals in children's stories and toys underwent dramatic transformation over the years 1830-1915. During the earlier part of that period, wild animals were presented to children as being savage and dangerous, and that it was necessary for them to be killed or brutally constrained. In the 1890s, an animalcentric discourse emerged in Nature writing, along with an animal-human symbiosis in scientific child study that highlighted childhood innocence, resulting in a valuing of wild animals based upon their similarity to humans. This article will describe the aesthetic devices of children's stories and play materials in relation to the dominant, emerging, and residual ideas about the wild communicated by adults to children through these means.
Birdsong and the Image of Evolution
Authors: Mundy Rachel 
Published: June 2009

AbstractFor nearly a quarter of Darwin's Descent of Man (1871), it is the singing bird whose voice presages the development of human aesthetics. But since the 1950s, aesthetics has had a perilous and contested role in the study of birdsong. Modern ornithology's disillusionment with aesthetic knowledge after World War II brought about the removal of musical studies of birdsong, studies which were replaced by work with the sound spectrograph, a tool that changes the elusive sounds of birdsong into a readable graphic image called a spectrogram. This article narrates the terms under which the image, rather than the sound, of birdsong has become a sign of humanity's ability to reason objectively. Drawing examples from the strange evolutionary tales that exist at the juncture of ornithology, music history, illustration, and linguistics, this story suggests how it was that the human ear disappeared in the unbridgeable gap between the sciences and the study of aesthetics so tellingly termed "the humanities."
Sociology and Beyond: Cultivating an Ontological Epistemology of Participation
Authors: Giri Ananta Kumar 
Published: June 2009

AbstractSociology reflects some of the fundamental assumptions of modernity. As an enterprise of knowledge, sociology reflects the modernistic privileging of epistemology to the neglect of ontological cultivation. The present article seeks to go beyond some of our modernistic limitations and cultivate sociology as a creative engagement in ontological epistemology of participation.
Exploring Practical Horizons of Beyond Sociology: Structure, Agency, and Strategy among Tenants in India
Authors: Olsen Wendy 
Published: June 2009

AbstractA theoretical orientation toward 'strategies' is proposed in this paper. This theoretical approach suggests that we study both the context for immediate decision-making and the broader context in which reflection and deliberation occur. Whilst these are very broad concerns, the paper itself is based on fieldwork about land rental relationships in rural South India. The paper introduces strategies as a solution to the theorists' dilemma of choice vs. constraints. We treat tenants (as both households and as individual agents) in their structural contexts whilst respecting the complexity and co-mutuality of their agency. The strategies that people use involve an orientation to current and future events, including possible events which are imagined or which could happen. Both structural relationships and concrete past incidents act as reference points for decisions made today in a given relationship. The strategies of tenants include being pliable vis-à-vis landlords but some tenants make this conditional upon landlords' proper behaviour. Agents negotiate and enforce proper behaviour and, thus, both create and change the system of norms that exists. In Macintyre's terms (1985), the virtues intrinsic to the socio-economic practices are continually being re-worked. The theory of strategies reframes 'virtue' in dynamic structure-and-agency terms. Agents are not simply individuals. The debate about rational choice vs. holism can be augmented by looking at agency supra-individualistically. The strategy of a household is an emergent property of the household as an agent. It includes detailed first-order strategies along with more reflective second-order strategies which reconcile goals in the education, migration, household work, and marriage domains. The paper is, thus, interdisciplinary and contributes to sociology, while pluralistically drawing upon other disciplines.
What is Social Capital? A Comprehensive Review of the Concept
Authors: Bhandari Humnath , Yasunobu Kumi 
Published: June 2009

AbstractSocial capital is an old concept but it entered into academic and policy debates only in 1990s. Its importance in explaining economic and social phenomena have been increasingly felt in recent years. Literature on theoretical and empirical aspects of social capital grew significantly during last decade. The whole notion of social capital is centred on social relationships and its major elements include social networks, civic engagement, norms of reciprocity, and generalised trust. Broadly speaking, it is defined as a collective asset in the form of shared norms, values, beliefs, trust, networks, social relations, and institutions that facilitate cooperation and collective action for mutual benefits. It is a complex multidimensional concept having different dimensions, types, and levels of measurement. Common types of social capital include: structure and cognitive; bonding, bridging, and linking; strong and weak; and horizontal and vertical. It can be measured and analysed at individual- and collective-levels in terms of social perspective and micro-, meso- and macro-levels in terms of geographic perspective. The properties of social capital, such as capacity to appear in as an explanatory variable in the production function, accumulation over time, capability of improving economic performance, investment with expected future returns, convertibility, and the need of maintenance, make it qualify as a form of capital, though there are some criticisms about the use of term 'capital' in social capital. Research on social capital remains in its initial stage and the concept is still elusive, prone to contextual definition, deficient in common measurement indicators, inability to explicitly quantify effects, and subject to various criticisms. Conceptual and measurement imprecision has led the concept prone to vague interpretation, less empirical application, and underestimation of its value. More empirical studies and testing of the concept on the ground is needed to develop a commonly accepted definition and measurement indicators that can explicitly disentangle and quantify its effects on overall development processes. Better conceptualisation and operationalisation of social capital theory is helpful to attract more investment on its development, design appropriate social policies, and promote sustainable development.
China's Pragmatic Approach to Modernisation
Authors: Michael Heng Siam Heng 
Published: June 2009

AbstractThe book studies China's record of modernization in its own terms. The course charted by the Chinese leadership has certainly produced remarkable successes. It is a journey of economic reforms based on pragmatism, and the same thinking is guiding the ongoing legal reforms. There is no denial that the leaders are reluctant to embark on radical political reforms that may unseat them. At the same time, one must be informed by the historical experience of the Chinese people. The havocs and sufferings associated with radical political changes have made them risk averse, and the ruling Communist Party of China is shrewd enough to exploit this feeling. Based on empirical data on countries with similar income level, Peerenboom argues for democratic reform to occur after a certain level of economic development. This point is controversial but there is inadequate data to disprove him. However, even with the limitation of one-party rule, there are a few areas where the CPC can do a better job in bringing China further along the road to modernization.
Cougar and jaguar habitat use and activity patterns in central Mexico
Authors: Monroy-Vilchis Octavio , Rodríguez-Soto Clarita , Zarco-González Martha , Urios Vicente 
Published: June 2009

Abstract
In this study the habitat use and activity patterns of the two of the largest cats of the Americas in central Mexico were studied. Three ways to detect felid presence were employed from August 2002 to May 2006: interviews, signs, and camera-traps. 478 records were obtained, from which 441 were from cougar and 37 from jaguar. These records included positive response in 118 of 140 interviews and 236 records of signs (mainly tracks and scats), and 124 photographs. Both felids preferred pine-oak forest habitats, with altitudes higher than 1800 m, distances between 3509 and 4377 m from roads, between 2329 and 4650 m from settlements, and distances to very steep slopes between 1048 and 2059 m, for jaguar, and for cougar lower than 1047 m. Jaguar activity was recorded mainly during nighttimes, between 0:00 and 6:00, whereas cougar was active between 4:00 and 6:00 and between 18:00 and 22:00 hours, avoiding the jaguar's principal activity period.
The effects of chronic administration of ghrelin on rat sperm quality and membrane integrity
Authors: Kheradmand Arash , Taati Majid , Babaei Homayoon 
Published: June 2009

AbstractAlthough ghrelin acts as a modulator of feeding behavior and energy metabolism in the central nervous system, recent studies have implicated the peripheral actions of ghrelin in reproductive tissues. Here, we investigated the effects of chronic administration of ghrelin on the motility, plasma membrane integrity and concentration of rat spermatozoa. 45-d male Wistar rats were scheduled for the study and were divided into control and treatment groups. In the treatment group, 1 nmol of ghrelin was administered as sc injection for 10 consecutive days or vehicle (physiological saline) to the control rats. Sperm collection was achieved by killing of the rats on days 15, 25 and 50 after first injection. Total sperm motility and forward progressive movement did not exhibit significant difference during the experiment, although, there was a tendency for greater motion rate on d 15 and 25 in the treated rats compared to the control group. Plasma membrane integrity (HOS-reacted spermatozoa) was significantly higher in the treated animals, especially on day 15 as well as day 25, because of possible antioxidant properties of ghrelin. This value was statistically higher on day 15 than that of day 25 (P <0.05). Likewise, there was a significant correlation between the FPM (P <0.0001, r = 0.79) and TSM (P <0.01, r = 0.52) with the HOS test percentage in the treatment group. It was not observed statistically difference in the sperm concentration between groups during all of the experimental days. In conclusion, chronic administration of ghrelin (similar to induced by energy deficiency such as fasting) increased the integrity of sperm membrane, however, the sperm motility and concentration did not display any alterations.