bluuebunny/crossref_metadata_2025_split huggingface dataset:
Associations of Occupational Tasks with Knee and Hip Osteoarthritis: The Johnston County Osteoarthritis Project
Authors: ALLEN KELLI D. , CHEN JIU-CHIUAN , CALLAHAN LEIGH F. , GOLIGHTLY YVONNE M. , HELMICK CHARLES G. , RENNER JORDAN B. , JORDAN JOANNE M. 
Published: February 2010

Objective.This cross-sectional study examined associations of occupational tasks with radiographic and symptomatic osteoarthritis (OA) in a community-based sample.Methods.Participants from the Johnston County Osteoarthritis Project (n = 2729) self-reported the frequency of performing 10 specific occupational tasks at the longest job ever held (never/seldom/sometimes vs often/always) and lifetime exposure to jobs that required spending > 50% of their time doing 5 specific tasks or lifting 22, 44, or 110 pounds 10 times weekly. Multivariable logistic regression models examined associations of each occupational task separately with radiographic and symptomatic knee and hip OA, controlling for age, race, gender, body mass index, prior knee or hip injury, and smoking.Results.Radiographic hip and knee OA were not significantly associated with any occupational tasks, but several occupational tasks were associated with increased odds of both symptomatic knee and hip OA: lifting > 10 pounds, crawling, and doing heavy work while standing (OR 1.4–2.1). More occupational walking and standing and less sitting were also associated with symptomatic knee OA, and more bending/twisting/reaching was associated with symptomatic hip OA. Exposure to a greater number of physically demanding occupational tasks at the longest job was associated with greater odds of both symptomatic knee and hip OA.Conclusion.Our results confirm an association of physically demanding occupational tasks with both symptomatic knee and hip OA, including several specific activities that increased the odds of OA in both joint groups. These tasks represent possibilities for identifying and targeting at-risk individuals with preventive interventions.
Single Coronary Artery: Right Coronary Artery Originated From Middle of Left Anterior Descending Artery in a Patient With Severe Mitral Regurgitation
Authors: Meric Murat , Demircan Sabri , Pinar Mesut , Yuksel Serkan 
Published: February 2010

AbstractThe single coronary artery is a benign and very rare coronary artery abnormality. Anomalous origin of the right coronary artery originating from the left anterior descending artery has been reported previously in just a few cases. In this article, we presented a patient with an anomalous origin of the right coronary artery from the midportion of the left anterior descending artery. The anomalous coronary artery was discovered incidentally during a coronary angiography performed prior to mitral valve surgery. Copyright © 2010 Wiley Periodicals, Inc.
Radiation monotherapy as regional treatment for lymph node‐positive Merkel cell carcinoma
Authors: Fang L. Christine , Lemos Bianca , Douglas James , Iyer Jayasri , Nghiem Paul 
Published: February 2010

AbstractBACKGROUND:Merkel cell carcinoma (MCC) is an aggressive cutaneous malignancy with a high risk of lymph node involvement. To the authors' knowledge, few data have been published to date regarding the optimal regional therapy for lymph node‐positive patients. This cohort study was performed to analyze the outcomes of patients with lymph node‐positive MCC treated with lymph node irradiation as definitive therapy compared with completion lymphadenectomy (CLND).METHODS:Fifty patients with lymph node involvement of MCC at presentation and adequate follow‐up data were included in this analysis. Forty‐three of these patients were enrolled and followed prospectively. Twenty‐six patients presented with microscopic lymph node disease, and 24 patients presented with palpable lymph node involvement.RESULTS:Regional control for patients with microscopically involved lymph nodes was 100% regardless of treatment modality—definitive lymph node irradiation (n = 19) or CLND ± radiotherapy (n = 7) with median follow‐up of 18 months. Patients with clinically positive lymph nodes had 2‐year regional recurrence‐free survival rate of 78% and 73% in the definitive lymph node irradiation (n = 9) and CLND ± radiotherapy (n = 15) groups, respectively (P = .8) with a median follow‐up of 16 months.CONCLUSIONS:To the best of the authors' knowledge, the current study is the largest series published to date of radiation monotherapy as regional treatment for lymph node‐positive MCC. Lymph node irradiation alone to positive regional lymph nodes was found to confer an excellent regional control rate that was comparable to CLND for both microscopic and palpable lymph node disease. There was no difference noted with regard to overall survival. Given their similar efficacy, the choice between these lymph node therapies may be based on the clinical scenario and anticipated side effect profiles. Cancer 2010. © 2010 American Cancer Society.
Antineoplastic therapy use in patients with advanced cancer admitted to an acute palliative care unit at a comprehensive cancer center
Authors: Hui David , Elsayem Ahmed , Li Zhijun , De La Cruz Maxine , Palmer J. Lynn , Bruera Eduardo 
Published: February 2010

AbstractBACKGROUND:Cancer patients admitted to a palliative care unit generally have a poor prognosis. The role of antineoplastic therapy (ANT) in these patients remains controversial. In the current study, the authors examined the frequency and predictors associated with ANT use in hospitalized patients who required admission to an acute palliative care unit (APCU).METHODS:Included in the study were all 2604 patients admitted over a 5‐year period to a 12‐bed APCU located within a National Cancer Institute comprehensive cancer center, in which patients had access to both palliative care and ANT. Institutional databases were used to retrospectively retrieve data regarding patient demographics, cancer diagnosis, ANT use, length of hospital stay, and survival from time of admission.RESULTS:The median hospital stay was 11 days, and the median survival was 22 days. During hospitalization, 435 patients (17%) received ANT, including chemotherapy (N = 297; 11%), hormonal agents (N = 54; 2%), and targeted therapy (N = 155; 6%). No significant change in the frequency of ANT use was detected over the 5‐year period. Multivariate logistic regression analysis revealed that younger age, specific cancer diagnoses, and longer admissions were independently associated with ANT use.CONCLUSIONS:The use of ANT during hospitalization that included an APCU stay was limited to a small percentage of patients and did not increase over time. ANT use was associated with younger age, specific cancer diagnoses, and longer admissions. The APCU facilitates simultaneous care for patients receiving ANT. Cancer 2010. © 2010 American Cancer Society.
Neurodevelopmental expression and localization of the cellular prion protein in the central nervous system of the mouse
Authors: Benvegnù Stefano , Poggiolini Ilaria , Legname Giuseppe 
Published: February 2010

AbstractTransmissible spongiform encephalopathies (TSEs) are neurodegenerative disorders caused by PrPSc, or prion, an abnormally folded form of the cellular prion protein (PrPC). The abundant expression of PrPC in the central nervous system (CNS) is a requirement for prion replication, yet despite years of intensive research the physiological function of PrPC still remains unclear. Several routes of investigation point out a potential role for PrPC in axon growth and neuronal development. Thus, we undertook a detailed analysis of the spatial and temporal expression of PrPC during mouse CNS development. Our findings show regional differences of the expression of PrP, with some specific white matter structures showing the earliest and highest expression of PrPC. Indeed, all these regions are part of the thalamolimbic neurocircuitry, suggesting a potential role of PrPC in the development and functioning of this specific brain system. J. Comp. Neurol. 518:1879–1891, 2010. © 2010 Wiley‐Liss, Inc.
A Highly Convergent Synthesis of Myristoyl‐carba(dethia)‐coenzyme A
Authors: Tautz Lutz , Rétey Janos 
Published: February 2010

AbstractCo‐translational myristoylation of the N‐terminal glycine residues of diverse signalling proteins is required for membrane attachment and proper function of these molecules. The transfer of myristate from myristoyl‐coenzyme A (myr‐CoA) is catalysed by the enzyme N‐myristoyltransferase (Nmt). Nmt has been implicated in a number of human diseases, including cancer and epilepsy, as well as in pathogenic mechanisms such as fungal and virus infections, including HIV and hepatitis B. Rational design has led to the development of potent competitive inhibitors, including several non‐hydrolysable acyl‐CoA substrate analogues. Linear synthetic strategies, following the route of the original CoA synthesis, however, generate such analogues in very low overall yields that typically are not sufficient for in vivo studies. Herewe present a new, highly convergent synthesis of myristoyl‐carba(dethia)‐coenzyme A (1) that allows this substrate analogue to be obtained in a yield 11 times higher than that of the reported linear synthesis. In addition, enzymatic cleavage of the adenosine‐2′,3′‐cyclophosphate in the last step of the synthesis proved to be an efficient way to obtain the isomerically pure 3′‐phosphate 1 free of the 2′‐phosphate 13.
Combining poly (methacrylic acid‐co‐ethylene glycol dimethacrylate) monolith microextraction and octadecyl phosphonic acid‐modified zirconia‐coated CEC with field‐enhanced sample injection for analysis of antidepressants in human plasma and urine
Authors: Wei Fang , Fan Jin , Zheng Ming‐Ming , Feng Yu‐Qi 
Published: February 2010

AbstractA method based on poly (methacrylic acid‐co‐ethylene glycol dimethacrylate) monolith microextraction and octadecylphosphonic acid‐modified zirconia‐coated CEC followed by field‐enhanced sample injection preconcentration technique was proposed for sensitive CE‐UV analysis of six antidepressants (doxepin, clozapine, imipramine, paroxetine, fluoxetine and chlorimipramine) in human plasma and urine. A poly(methacrylic acid‐co‐ethylene glycol dimethacrylate) monolithic capillary column was introduced for the extraction of antidepressants from urine and plasma samples. The hydrophobic main chains and acidic pendant groups of the monolithic column make it a superior material for extraction of basic analytes from aqueous matrix. After extraction, the desorption solvent, which normally provided an excellent medium to ensure direct compatibility for field‐enhanced sample injection in CE, was analyzed by CE directly. By the use of alkylphosphonate‐modified zirconia‐coated CEC for separation of the basic compounds of antidepressants, high separation efficiency and resolution were achieved because that both hydrophobic interaction between analytes and alkylphosphonate‐modified zirconia coat and electrophoretic effect work on the separation of antidepressants. The best separation was achieved using a buffer composed of 0.3 M ammonium acetate (adjusted to pH 4.5 with 1 M acetic acid) and 35% ACN v/v, with a temperature and voltage of 20°C and 20 kV, respectively. By applying both preconcentration procedures, LODs of 11.4–51.5 and 3.7–17.0 μg/L were achieved for the six antidepressants in human plasma and urine, respectively. Excellent method of reproducibility was found over a linear range of 50–5000 μg/L in plasma and urine sample.
Internal flow numerical simulation of a cross‐flow fan in refrigerant operating condition using user‐defined functions
Authors: Xue Yongfei , Gan Jiaye , Wang Jiabing , Wu Keqi 
Published: February 2010

AbstractIn the paper, a cross‐flow fan in refrigerant operating condition is systematically simulated using user‐defined functions. Three‐dimensional simulations are acquired with Navier–Stokes equations coupled with k–ε turbulence model, and internal flow characteristics of an indoor split‐type air conditioner are obtained, which is mainly composed of cross‐flow fan and heat exchanger. It has systematically been simulated in the isothermal flow condition that the performance of cross‐flow fan may be reduced easily with dry or humid air, and in the refrigerant operating condition in which user‐defined functions are applied to the humid air, considered as a mixture of dry air and vapor. A density‐modulated function is adopted to deal with the condensation of the vapor at the heat‐transfer region approximately. The results show flow mechanism of the two gas‐phase flow, including phase‐vary process. The distribution of the parameters is not uniform at the inlet of the machine, the intensity and position of pressure and velocity vary along the axial direction of the fan, the distribution of vapor volume fraction and turbulent intensity in heat‐transfer region is obtained, and the external characteristic data of the indoor machine are obtained and analyzed. Compared with the experimental data, the calculated characteristic curves and designed parameters are on target. © British Crown Copyright 2010/MOD. Reproduced with permission. Published by John Wiley & Sons, Ltd.
Optimal transcranial magnetic stimulation coil placement for targeting the dorsolateral prefrontal cortex using novel magnetic resonance image‐guided neuronavigation
Authors: Rusjan Pablo M. , Barr Mera S. , Farzan Faranak , Arenovich Tamara , Maller Jerome J. , Fitzgerald Paul B. , Daskalakis Zafiris J. 
Published: February 2010

AbstractThe dorsolateral prefrontal cortex (DLPFC) has been implicated in the pathophysiology of several psychiatric illnesses including major depressive disorder and schizophrenia. In this regard, the DLPFC has been targeted in repetitive transcranial magnetic stimulation (rTMS) studies as a form of treatment to those patients who are resistant to medications. The ‘5‐cm method’ and the ‘10‐20 method’ for positioning the transcranial magnetic stimulation (TMS) coil over DLPFC have been scrutinised due to poor targeting accuracies attributed to inter‐subject variability. We evaluated the accuracy of such methods to localise the DLPFC on the scalp in 15 healthy subjects and compared them with our novel neuronavigational method, which first estimates the DLPFC position in the cortex based on a standard template and then determines the most appropriate position on the scalp in which to place the TMS coil. Our neuronavigational method yielded a scalp position for the left DLPFC between electrodes F3 and F5 in standard space and was closest to electrode F5 in individual space. Further, we found that there was significantly less inter‐subject variability using our neuronavigational method for localising the DLPFC on the scalp compared with the ‘5‐cm method’ and the ‘10‐20 method’. Our findings also suggest that the ‘10‐20 method’ is superior to the ‘5‐cm method’ in reducing inter‐subject variability and that electrode F5 should be the stimulation location of choice when MRI co‐registration is not available. Hum Brain Mapp, 2010. © 2010 Wiley‐Liss, Inc.
Prognostic relevance of global histone H3 lysine 4 (H3K4) methylation in renal cell carcinoma
Authors: Ellinger Jörg , Kahl Philip , Mertens Claudia , Rogenhofer Sebastian , Hauser Stefan , Hartmann Wolfgang , Bastian Patrick J. , Büttner Reinhard , Müller Stefan C. , von Ruecker Alexander 
Published: February 2010

AbstractEpigenetic alterations play an important role in carcinogenesis. Recent studies suggested that global histone modifications are predictors of cancer recurrence in various tumor entities. Our study was performed to evaluate histone H3 lysine 4 mono‐methyl (H3K4me1), ‐di‐methyl (H3K4me2) and ‐trimethyl (H3K4me3) patterns in renal cell carcinoma (RCC) using a tissue microarray with 193 RCC (including 142 clear cell, 31 papillary, 10 chromophobe and 10 sarcomatoid RCC) and 10 oncocytoma specimens: H3K4me3 staining was more intense in papillary RCC, whereas H3K4me1 and H3K4me2 were similar in the diverse RCC subtypes. H3K4me2 and H3K4me3 levels were increased in oncocytoma. H3K4me1‐3 levels were inversely correlated with Fuhrman grading, pT stage, lymph node involvement and distant metastasis. Progression‐free survival and cancer‐specific survival were shorter in patients with low levels of H3K4me1‐3 in the univariate analysis, but we did not observe a significant correlation of a single modification in a multivariate model, which also included the established prognostic parameters TNM‐stage and Fuhrman grade. In comparison, the H3K4me score, which combined staining levels of the H3K4 modifications, was an independent predictor of RCC progression‐free survival. Our study on H3K4 methylation supports the concept of global histone modifications as potential cancer prognosis markers.
MUC12 mRNA expression is an independent marker of prognosis in stage II and stage III colorectal cancer
Authors: Matsuyama Takatoshi , Ishikawa Toshiaki , Mogushi Kaoru , Yoshida Tsuyoshi , Iida Satoru , Uetake Hiroyuki , Mizushima Hiroshi , Tanaka Hiroshi , Sugihara Kenichi 
Published: February 2010

AbstractDistant metastasis is the major cause of death in colorectal cancer (CRC) patients. To identify genes influencing the prognosis of patients with CRC, we compared gene expression in primary tumors with and without distant metastasis using an oligonucleotide microarray. We also examined the expression of the candidate gene in 100 CRC patients by quantitative real‐time reverse transcription PCR and studied the relationship between its expression and the prognosis of patients with CRC. As a result, we identified MUC12 as a candidate gene involved in metastasis processes by microarray analysis. Quantitative real‐time reverse transcription PCR showed that MUC12 expression was significantly lower in cancer tissues than in adjacent normal tissues (p < 0.001). In Stages II and III CRC, patients with low expression showed worse disease‐free survival (p = 0.020). Multivariate analysis disclosed that MUC12 expression status was an independent prognostic factor in Stages II and III CRC (relative risk, 8.236; 95% confidence interval, 1.702–39.849 p = 0.009). Our study revealed the prognostic value of MUC12 expression in CRC patients. Moreover, our result suggests MUC12 expression is a possible candidate gene for assessing postoperative adjuvant therapy for CRC patients.
Parallel transmit and receive technology in high‐field magnetic resonance neuroimaging
Authors: Webb Andrew G. , Collins Christopher M. 
Published: February 2010

AbstractThe major radiofrequency engineering challenges of high‐field MR neuroimaging are as follows: (1) to produce a strong, homogeneous transmit B1 field, while remaining within regulatory guidelines for tissue power deposition and (2) to receive the signal with the maximum signal‐to‐noise and the greatest flexibility in terms of utilizing the benefits of parallel imaging. Borrowing from developments in electromagnetic hyperthermia, the first challenge has been met by the use of transmit arrays, in which the input power to each element of the array can be varied in terms of magnitude and phase. Optimization of these parameters, as well as the form of the applied RF pulse, leads to very homogeneous B1 fields throughout the brain. The design of large receive arrays, using impedance‐mismatched preamplifiers and geometrical overlap for interelement isolation, has resulted in significant sensitivity improvements as well as large acceleration factors in parallel imaging. © 2010 Wiley Periodicals, Inc. Int J Imaging Syst Technol, 20, 2–13, 2010
Stimulation of glucocorticoid‐induced tumor necrosis factor receptor family‐related protein ligand (GITRL) induces inflammatory activation of microglia in culture
Authors: Hwang Heehong , Lee Shinrye , Lee Won‐Ha , Lee Ho‐Jae , Suk Kyoungho 
Published: February 2010

AbstractGlucocorticoid‐induced tumor necrosis factor receptor family‐related protein ligand (GITRL) is a member of the tumor necrosis factor superfamily (TNFSF) and is known to act as a costimulator in the immune system by binding to GITR. GITRL is expressed in endothelial cells, dendritic cells, macrophages, and B cells, but it is not known whether GITRL is expressed in brain microglia cells. Here, we investigated the expression of GITR and GITRL and their potential role in microglia cells. Using BV‐2 mouse microglia cells and mouse primary microglia cultures, we have demonstrated that 1) both GITR and GITRL are expressed in microglia cells; 2) stimulation of GITRL induces inflammatory activation of microglia on the basis of production of nitric oxide (NO) and expression of inducible nitric oxide synthase, cyclooxygenase‐2, CD40, and matrix metalloproteinase‐9; 3) GITRL‐mediated microglial NO production partially depends on p38 MAPK, JNK, and nuclear factor‐κB pathways; and 4) GITRL stimulation also induces microglia cell death. These results indicate that GITR and GITRL are functionally expressed on brain microglia and that the stimulation of GITRL can induce inflammatory activation of microglia. The GITR/GITRL system may play an important role in neuroinflammation. © 2010 Wiley‐Liss, Inc.
Matrix metalloproteinase (MMP)‐3 gene up‐regulation in a rat tail compression loading‐induced disc degeneration model
Authors: Yurube Takashi , Nishida Kotaro , Suzuki Teppei , Kaneyama Shuichi , Zhang Zhongying , Kakutani Kenichiro , Maeno Koichiro , Takada Toru , Fujii Masahiko , Kurosaka Masahiro , Doita Minoru 
Published: February 2010

AbstractThe rodent static compression loading‐induced disc degeneration model still has important gaps among the radiographic, magnetic resonance imaging (MRI), and histological schemes and the acute and chronic expression of catabolic genes such as matrix metalloproteinase (MMP)‐3. Our objectives were to assess the validity of a rat tail two‐disc static compression model and to elucidate a representative catabolic marker, MMP‐3 gene alterations, throughout the degenerative process. Static compression at 1.3 MPa for up to 56 days produced progressive disc height loss in radiographs, lower nucleus intensity on T2‐weighted MRIs, and histomorphological degeneration. Real‐time RT‐PCR mRNA quantification showed significant MMP‐3 up‐regulation in nucleus pulposus cells from 7 days and a significantly progressive increase as the loading duration lengthened, with high correlations to radiological degenerative scores. Immunohistochemistry demonstrated progressively increased positive staining for MMP‐3. These results validate this animal model for disc degeneration research. Progressive mRNA and protein‐distributional up‐regulations indicate the significant role of MMP‐3 and its feasibility as a disc degenerative marker. This model should prove useful for investigating the pathomechanism and for evaluating molecular therapies for degenerative disc disease. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1026–1032, 2010
Activation of the aromatic system by the SO2CF3 group: Kinetics study and structure–reactivity relationships
Authors: Guesmi Nizar El , Boubaker Taoufik , Goumont Régis 
Published: February 2010

AbstractThe rate constants for the reaction of 2,6‐bis(trifluoromethanesulfonyl)‐4‐nitroanisole with some substituted anilines have been measured by spectrophotometric methods in methanol at various temperatures. The data are consistent with the SNAr mechanism. The effect of substituents on the rate of reaction has been examined. Good linear relationships were obtained from the plots of log k1 against Hammett σpara constants values at all temperature with negative ρ values (−1.68 to −1.11). Activation parameters Δ≠H varied from 41.6 to 54.3 kJ mol−1 and Δ≠S from −142.7 to −114.6 J mol−1 K−1. The δΔ≠H and δΔ≠S reaction constants were determined from the dependence of Δ≠H and Δ≠S activation parameters on the σ substituent constants, by analogy with the Hammett equation. A plot of Δ≠H versus Δ≠S for the reaction gave good straight line with 177°C isokinetic temperature. © 2010 Wiley Periodicals, Inc. Int J Chem Kinet 42: 203–210, 2010