bluuebunny/crossref_metadata_2025_split huggingface dataset:
Immunohistochemical characterization of calcitonin gene-related peptide in the trigeminal system of the familial hemiplegic migraine 1 knock-in mouse
Authors: Mathew Rammya , Andreou Anna P , Chami Linda , Bergerot Astrid , van den Maagdenberg Arn MJM , Ferrari Michel D , Goadsby Peter J 
Published: September 2011

Introduction: Familial hemiplegic migraine type 1 (FHM-1) is caused by mutations in the CACNA1A gene, with the R192Q mutation being the most common. Elevated calcitonin gene-related peptide (CGRP) levels in acute migraine and clinical trials using CGRP receptor antagonists suggest CGRP-related mechanisms are important in migraine. Methods: Wild-type and R192Q knock-in mice were anaesthetized and perfused. Using immunohistochemical staining, the expression of CGRP in the trigeminocervical complex (TCC) and in the trigeminal and dorsal root ganglia was characterized. Results: There was a 38% reduction in the percentage of CGRP-immunoreactive cells in the trigeminal ganglia ( p < 0.001) of R192Q knock-in mice compared to wild-type animals. The size distribution profile of CGRP-immunoreactive cells within the trigeminal ganglia demonstrated no significant difference in cell diameter between the two groups ( p ≥ 0.56). CGRP expression was also reduced in thoracic ganglia of R192Q knock-in mice (21% vs. 27% in wild-type group; p < 0.05), but not in other ganglia. In addition, decreased CGRP immunoreactivity was observed in the superficial laminae of the TCC in R192Q knock-in mice, when compared to the control group ( p < 0.005). Conclusion: The data demonstrates that the FHM-1 CACNA1A mutation alters CGRP expression in the trigeminal ganglion and TCC. This suggests further study of these animals is warranted to characterize better the role of these mutations in the neurobiology of migraine.
A new interpretation of Jensen's inequality and geometric properties of ϕ-means
Authors: Nakasuji Yasuo , Kumahara Keisaku , Takahasi Sin-Ei 
Published: September 2011

Abstract
We introduce a mean of a real-valued measurable function f on a probability space induced by a strictly monotone function φ. Such a mean is called a φ-mean of f and written by M

φ
(f). We first give a new interpretation of Jensen's inequality by φ-mean. Next, as an application, we consider some geometric properties of M

φ
(f), for example, refinement, strictly monotone increasing (continuous) φ-mean path, convexity, etc.

Mathematics Subject Classification (2000): Primary 26E60; Secondary 26B25, 26B05.
Risk factors for respiratory work disability in a cohort of pulp mill workers exposed to irritant gases
Authors: Murgia Nicola , Torén Kjell , Kim Jeong-Lim , Andersson Eva 
Published: September 2011

Abstract

Background
The association between chronic respiratory diseases and work disability has been demonstrated a number of times over the past 20 years, but still little is known about work disability in occupational cohorts of workers exposed to respiratory irritants. This study investigated job or task changes due to respiratory problems as an indicator of work disability in pulp mill workers occupationally exposed to irritants.

Methods
Data about respiratory symptoms and disease diagnoses, socio-demographic variables, occupational exposures, gassing episodes, and reported work changes due to respiratory problems were collected using a questionnaire answered by 3226 pulp mill workers. Information about work history and departments was obtained from personnel files. Incidence and hazard ratios for respiratory work disability were calculated with 95% confidence intervals (CI).

Results
The incidence of respiratory work disability among these pulp mill workers was 1.6/1000 person-years. The hazard ratios for respiratory work disability were increased for workers reporting gassings (HR 5.3, 95% CI 2.7-10.5) and for those reporting physician-diagnosed asthma, chronic bronchitis, and chronic rhinitis, when analyzed in the same model.

Conclusions
This cohort study of pulp mill workers found that irritant peak exposure during gassing episodes was a strong predictor of changing work due to respiratory problems, even after adjustment for asthma, chronic bronchitis, and chronic rhinitis.
C lamp-Cru shin g versus s tapler h epatectomy for transection of the parenchyma in elective hepatic resection (CRUNSH) - A randomized controlled trial (NCT01049607)
Authors: Rahbari Nuh N , Elbers Heike , Koch Moritz , Bruckner Thomas , Vogler Patrick , Striebel Fabian , Schemmer Peter , Mehrabi Arianeb , Büchler Markus W , Weitz Jürgen 
Published: September 2011

Abstract

Background
Hepatic resection is still associated with significant morbidity. Although the period of parenchymal transection presents a crucial step during the operation, uncertainty persists regarding the optimal technique of transection. It was the aim of the present randomized controlled trial to evaluate the efficacy and safety of hepatic resection using the technique of stapler hepatectomy compared to the simple clamp-crushing technique.

Methods/Design
The CRUNSH Trial is a prospective randomized controlled single-center trial with a two-group parallel design. Patients scheduled for elective hepatic resection without extrahepatic resection at the Department of General-, Visceral- and Transplantation Surgery, University of Heidelberg are enrolled into the trial and randomized intraoperatively to hepatic resection by the clamp-crushing technique and stapler hepatectomy, respectively. The primary endpoint is total intraoperative blood loss. A set of general and surgical variables are documented as secondary endpoints. Patients and outcome-assessors are blinded for the treatment intervention.

Discussion
The CRUNSH Trial is the first randomized controlled trial to evaluate efficacy and safety of stapler hepatectomy compared to the clamp-crushing technique for parenchymal transection during elective hepatic resection.

Trial Registration
ClinicalTrials.gov: NCT01049607
Methyl-binding domain protein-based DNA isolation from human blood serum combines DNA analyses and serum-autoantibody testing
Authors: Wielscher Matthias , Pulverer Walter , Peham Johannes , Hofner Manuela , Rappaport Christine F , Singer Christian , Jungbauer Christof , Nöhammer Christa , Weinhäusel Andreas 
Published: September 2011

Abstract

Background
Circulating cell free DNA in serum as well as serum-autoantibodies and the serum proteome have great potential to contribute to early cancer diagnostics via non invasive blood tests. However, most DNA preparation protocols destroy the protein fraction and therefore do not allow subsequent protein analyses. In this study a novel approach based on methyl binding domain protein (MBD) is described to overcome the technical difficulties of combining DNA and protein analysis out of one single serum sample.

Methods
Serum or plasma samples from 98 control individuals and 54 breast cancer patients were evaluated upon silica membrane- or MBD affinity-based DNA isolation via qPCR targeting potential DNA methylation markers as well as by protein-microarrays for tumor-autoantibody testing.

Results
In control individuals, an average DNA level of 22.8 ± 25.7 ng/ml was detected applying the silica membrane based protocol and 8.5 ± 7.5 ng/ml using the MBD-approach, both values strongly dependent on the serum sample preparation methods used. In contrast to malignant and benign tumor serum samples, cell free DNA concentrations were significantly elevated in sera of metastasizing breast cancer patients. Technical evaluation revealed that serum upon MBD-based DNA isolation is suitable for protein-array analyses when data are consistent to untreated serum samples.

Conclusion
MBD affinity purification allows DNA isolations under native conditions retaining the protein function, thus for example enabling combined analyses of DNA methylation and autoantigene-profiles from the same serum sample and thereby improving minimal invasive diagnostics.
Progressive severe lung injury by zinc oxide nanoparticles; the role of Zn2+ dissolution inside lysosomes
Authors: Cho Wan-Seob , Duffin Rodger , Howie Sarah EM , Scotton Chris J , Wallace William AH , MacNee William , Bradley Mark , Megson Ian L , Donaldson Ken 
Published: September 2011

Abstract

Background
Large production volumes of zinc oxide nanoparticles (ZnONP) might be anticipated to pose risks, of accidental inhalation in occupational and even in consumer settings. Herein, we further investigated the pathological changes induced by ZnONP and their possible mechanism of action.

Methods
Two doses of ZnONP (50 and 150 cm2/rat) were intratracheally instilled into the lungs of rats with assessments made at 24 h, 1 wk, and 4 wks after instillation to evaluate dose- and time-course responses. Assessments included bronchoalveolar lavage (BAL) fluid analysis, histological analysis, transmission electron microscopy, and IgE and IgA measurement in the serum and BAL fluid. To evaluate the mechanism, alternative ZnONP, ZnONP-free bronchoalveolar lavage exudate, and dissolved Zn2+ (92.5 μg/rat) were also instilled to rats. Acridine orange staining was utilized in macrophages in culture to evaluate the lysosomal membrane destabilization by NP.

Results
ZnONP induced eosinophilia, proliferation of airway epithelial cells, goblet cell hyperplasia, and pulmonary fibrosis. Bronchocentric interstitial pulmonary fibrosis at the chronic phase was associated with increased myofibroblast accumulation and transforming growth factor-β positivity. Serum IgE levels were up-regulated by ZnONP along with the eosinophilia whilst serum IgA levels were down-regulated by ZnONP. ZnONP are rapidly dissolved under acidic conditions (pH 4.5) whilst they remained intact around neutrality (pH 7.4). The instillation of dissolved Zn2+ into rat lungs showed similar pathologies (eg., eosinophilia, bronchocentric interstitial fibrosis) as were elicited by ZnONP. Lysosomal stability was decreased and cell death resulted following treatment of macrophages with ZnONP in vitro.

Conclusions
We hypothesise that rapid, pH-dependent dissolution of ZnONP inside of phagosomes is the main cause of ZnONP-induced diverse progressive severe lung injuries.
Early onset sebaceous carcinoma
Authors: Sung Dongjin , Kaltreider Sara A , Gonzalez-Fernandez Federico 
Published: September 2011

Abstract

Background
Ocular sebaceous carcinoma can masquerade as benign lesions resulting in delay of diagnosis. Early recognition is even more difficult in young patients where the disease rarely occurs. Here, we provide a clinicopathological correlation of ocular sebaceous carcinoma in a young individual lacking history of hereditary cancer or immunosuppression.

Findings
A detailed histopathological study including p53 DNA sequencing was performed on an aggressive sebaceous carcinoma presenting in a healthy 32 year-old Caucasian woman. She had no history of retinoblastoma, evidence for a hereditary cancer syndrome, or radiation therapy. However, she potentially was at risk for excessive UV light exposure. A detailed review of the literature is also provided.
A moderately well differentiated sebaceous carcinoma was established histopathologically arising from the meibomian gland of the upper eyelid. In most areas, the cytoplasm contained small but distinct Oil-red-O positive vacuoles. Direct sequencing of p53 identified a G:C→A:T mutation at a dipyrimidine site. The mutation results in substitution of arginine for the highly conserved glycine at residue 199 located at the p53 dimer-dimer interface. Energy minimization structural modeling predicts that G199R will neutralize negative charges contributed by nearby inter- and intramonomeric glutamate residues.

Discussion
This study points to the importance of recognizing that sebaceous carcinoma can occur in young patients with no evidence for hereditary cancer risk or radiation therapy. The G199R substitution is anticipated to alter the stability of the p53 tetrameric complex. The role of UV light in the etiology of sebaceous carcinoma deserves further study. Our findings, taken together with those of others, suggest that different environmental factors could lead to the development of sebaceous carcinoma in different patients.

Virtual Slides
The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1227870450349458.
Abiotrophia defectiva knee prosthesis infection: A case report
Authors: Cassir Nadim , Grillo Jean-Charles , Argenson Jean-Noël , Drancourt Michel , Levy Pierre-Yves 
Published: September 2011

Abstract

Background

Abiotrophia species have rarely been implicated in osteoarticular infections. We report one case of an A. defectiva knee prosthesis infection.

Case presentation
A 71-year-old man of Italian origin presented with pain and swelling of the knee four years after the implantation of a total knee replacement prosthesis. While standard culturing of the synovial fluid resulted in no isolation of microorganisms, the direct inoculation of the synovial fluid into a rich culture medium resulted in the identification of A. defectiva by polymerase chain reaction sequencing. Repeated attempts of culturing microorganisms from blood were negative, and echocardiograms and colonoscopies were unremarkable. High-dose amoxicillin for nine months and a two-stage replacement of the knee prosthesis led to full patient recovery by the time of the 12-month follow-up examination.

Conclusions
Because Abiotrophia spp. are fastidious microorganisms, it is likely that cases of Abiotrophia orthopedic infection are misdiagnosed as culture-negative infections. Direct inoculation of synovial fluids into rich broth medium and further polymerase chain reaction-based detection of culture-negative synovial fluids are key tests for accurate documentation and detection of these infections.
Update: Hypogonadotropic Hypogonadism in Type 2 Diabetes and Obesity
Authors: Dandona Paresh , Dhindsa Sandeep 
Published: September 2011

Abstract
Studies over the last few years have clearly established that at least 25% of men with type 2 diabetes have subnormal free testosterone concentrations in association with inappropriately low LH and FSH concentrations. Another 4% have subnormal testosterone concentrations with elevated LH and FSH concentrations. The Endocrine Society, therefore, now recommends the measurement of testosterone in patients with type 2 diabetes on a routine basis. The subnormal testosterone concentrations are not related to glycosylated hemoglobin or duration of diabetes, but are associated with obesity, very high C-reactive protein concentrations, and mild anemia. In addition, subnormal testosterone concentrations in these men are associated with a two to three times elevated risk of cardiovascular events and death in two early studies. Short-term studies of testosterone therapy in hypogonadal men with type 2 diabetes have demonstrated an increase in insulin sensitivity and a decrease in waist circumference. However, the data on the effect of testosterone replacement on glycemic control and cardiovascular risk factors such as cholesterol and C-reactive protein concentrations are inconsistent. As far as sexual function is concerned, testosterone treatment increases libido but does not improve erectile dysfunction and thus, phosphodiesterase inhibitors may be required. Trials of a longer duration are clearly required to definitively establish the benefits and risks of testosterone replacement in patients with type 2 diabetes and low testosterone.
Screening for psychiatric illness among students of the University of The West Indies, Trinidad
Authors: Kameel Mungrue , Kamal Mohammed 
Published: September 2011

Abstract

Method: This cross-sectional observational study was performed in 2007 by convenience sampling of 1031 full-time undergraduate students registered in years 1, 2 and 3 in the academic year 2007/2008, administering a basic demographics questionnaire along with the standardised General Health Questionnaire-28 (GHQ-28) to each participant.

Results: The population of full-time students at the University of the West Indies (UWI) was found to be mentally healthy (median GHQ score was 5.00; GHQ=6 considered “mentally unhealthy”). However, median GHQ scores were lowest (4.00) in the Faculties of Medical Sciences and Social Sciences, but highest (6.00) in the Faculty of Humanities and Education. Furthermore, analysis showed that females were less mentally healthy than males attending the UWI. With regard to ethnicity, the African group was the most mentally healthy group whereas the group of mixed ethnicity was the least. Religion was also shown to significantly affect the GHQ (4.00 for Christians and Hindus, 6.00 for Muslims and 8.00 for those categorized as ‘other’). Most interesting was the relationship of GHQ scores with the manner in which participants related to their lecturers, classmates, guardians, siblings and friends; it was found that the better one related to these people, the lower one’s GHQ score was and hence one was considered more mentally healthy.

Conclusions: UWI full-time undergraduate students are mentally healthy, but gender, ethnicity, religion, quality of relationships and faculty of study still contribute to significant differences within the population. This allows high-risk individuals to be identified and indicates where steps can be taken to improve mental health.
Uniqueness in inverse scattering of elastic waves by three-dimensional polyhedral diffraction gratings
Authors: Elschner Johannes , Hu Guanghui 
Published: September 2011

Abstract
We consider the inverse elastic scattering problem of determining a three-dimensional diffraction grating profile from scattered waves measured above the structure. In general, a grating profile cannot be uniquely determined by a single incoming plane wave. We completely characterize and classify the bi-periodic polyhedral structures under the boundary conditions of the third and fourth kinds that cannot be uniquely recovered by only one incident plane wave. Thus we have global uniqueness for a polyhedral grating profile by one incident elastic plane wave if and only if the profile belongs to neither of the unidentifiable classes, which can be explicitly described depending on the incident field and the type of boundary conditions. Our approach is based on the reflection principle for the Navier equation and the reflectional and rotational invariance of the total field.
Evaluation of Particle Shape and Surface Texture of Recycled Organic Aggregates
Authors: Pierce Charles E. , Brown Larry R. 
Published: September 2011

Abstract
The size, shape, and texture of aggregate particles are important because these physical characteristics influence the engineering properties. Recycled organic aggregates have advantageous physical and mechanical properties for geoengineering applications, but the particle shapes and textures have not been well quantified and evaluated. Three pairs of recycled organic aggregates were studied in this investigation: nylon and polyethylene terephthalate (thermoplastics), ground and crumb rubber (elastomers), and pine and oak wood (natural organic materials). The particle flatness, elongation, and sphericity were calculated for representative samples of each aggregate. The particle index of the shape and texture was measured per ASTM D 3398, and this test method was determined to provide a meaningful assessment of irregular geometrical characteristics. With the exception of nylon chips, recycled organic aggregates contain a high percentage of flat and elongated particles with Lmax:Lmin ratios that are much greater than those observed in crushed stone, gravel, and sand. Void volumes are much higher, resulting in particle indices between 30 and 50, as compared to indices of less than 20 for natural, inorganic aggregate.
The BTB-ZF Family of Transcription Factors: Key Regulators of Lineage Commitment and Effector Function Development in the Immune System
Authors: Beaulieu Aimee M. , Sant'Angelo Derek B. 
Published: September 2011

Abstract
Successful immunity depends upon the activity of multiple cell types. Commitment of pluripotent precursor cells to specific lineages, such as T or B cells, is obviously fundamental to this process. However, it is also becoming clear that continued differentiation and specialization of lymphoid cells is equally important for immune system integrity. Several members of the BTB-ZF family have emerged as critical factors that control development of specific lineages and also of specific effector subsets within these lineages. For example, BTB-ZF genes have been shown to control T cell versus B cell commitment and CD4 versus CD8 lineage commitment. Others, such as PLZF for NKT cells and Bcl-6 for T follicular helper cells, are necessary for the acquisition of effector functions. In this review, we summarize current findings concerning the BTB-ZF family members with a reported role in the immune system.
Triple Therapy for Corneal Neovascularization: A Case Report
Authors: Veritti Daniele , Vergallo Stefano , Lanzetta Paolo 
Published: September 2011

Purpose To investigate the effect of the combination of photodynamic therapy with verteporfin and subconjunctival bevacizumab and triamcinolone acetonide on corneal neovascularization secondary to corneal graft failure. Methods A patient with extensive deep and superficial corneal neovascularization underwent same day triple therapy with subconjunctival bevacizumab (1.25 mg), subconjunctival triamcinolone acetonide (40 mg), and photodynamic therapy with verteporfin (fluence 50 J/cm2, irradiance 300 mW/cm2). Results A complete angiographic new vessel regression was obtained and was discernible starting 7 days after combination therapy. Three months after treatment, an initial new-vessel sprout was noted. Triple therapy was then repeated. No angiographic evidence of corneal neovascularization was detectable for the entire 6-month follow-up duration. Conclusions Triple therapy may offer a promising tool in the treatment of corneal neovascularization.
Organo‐ and Hydrogelators Based on Luminescent Monocationic Terpyridyl Platinum(II) Complexes with Biphenylacetylide Ligands
Authors: Wang Jia , Chen Yong , Law Yuen‐Chi , Li Meiyi , Zhu Ming‐Xin , Lu Wei , Chui Stephen Sin‐Yin , Zhu Nianyong , Che Chi‐Ming 
Published: September 2011

AbstractA series of phosphorescent terpyridyl platinum(II) complexes with ancillary biphenylacetylide ligands, namely, [(R3tpy)PtC≡C(biphenyl)]X (R=tBu, H, or Et2N; tpy=2,2′;6′,2′′‐terpyridyl; X is an anion) were synthesized and structurally characterized by various spectroscopic techniques and X‐ray diffraction methods. Despite a lack of long alkyl chain(s) or hydrogen‐bonding motif(s), complexes [(tpy)PtC≡C(biphenyl)]Cl and [(tBu3tpy)PtC≡C(biphenyl)]X (X=Cl, ClO4, PF6, or BF4) were found to gelate water and organic solvents, respectively. The self‐aggregation of these complexes in solutions and the resulting gels were investigated with variable‐temperature (VT) 1H NMR spectroscopy, polarized optical microscopy, and absorption/emission spectroscopy. SEM micrographs on dry gels revealed entangled nanofibers with diameters of 20–40 nm and lengths of tens of micrometers. Powder X‐ray diffraction (PXRD) study revealed various degrees of crystallinity of these fibrillar nanostructures. The substituents on both the terpyridyl and acetylide ligands and counterion of these complexes play a profound but concerted role in tuning the intermolecular metal⋅⋅⋅metal and/or π–π interactions, and hence the gelation properties.