bluuebunny/crossref_metadata_2025_split huggingface dataset:
Education of adult type 1 diabetes patients in a diabetes ward setting: a best practice implementation project
Authors: Kania Michał , Suduł Paulina , Wilk Magdalena , Szopa Magdalena , Katra Barbara , Małecki Maciej T. , Poklepović Peričić Tina , Prill Robert , Klugarová Jitka , Vrbova Tereza , Klugar Miloslav , Leśniak Wiktoria , Bała Małgorzata M. 
Published: June 2024

ABSTRACT

Introduction and objectives:
Type 1 diabetes is an autoimmune disease that destroys insulin-producing cells in the pancreas. Education is the cornerstone of effective diabetes care. In this implementation project, we aimed to improve compliance with best practices regarding type 1 diabetes educational interventions for adult hospitalized patients.

Methods:
This project was guided by the JBI Evidence Implementation Framework. A baseline audit was conducted involving 20 nurses and 20 type 1 diabetes patients who received regular educational measures. Areas of non-compliance were identified and an improvement strategy was implemented. A follow-up audit was then conducted to evaluate the effectiveness of the improvement strategy. The project was conducted in Poland in 2021 in a tertiary referral unit that specializes in the diagnosis and treatment of diabetes.

Results:
Substantial improvements were noted for all audit criteria after the implementation of strategies to address areas of non-compliance. Use of the education program improved from 0% to 100%. Compliance regarding patients receiving handouts and personalization of the program increased to 100%. We observed a significant improvement from 0% to 80% in the structuring of the program content.

Conclusions:
This project successfully improved the quality of education provided for type 1 diabetes patients in all relevant areas. We devised an education program, covering important aspects of diabetes education, with the patients reporting increased satisfaction with the personalized educational measures during their hospital stay.

Spanish abstract:

http://links.lww.com/IJEBH/A215
Rapid and prolonged antidepressant and antianxiety effects of psilocybin, lysergic acid diethylamide, ayahuasca, and 3, 4-methylenedioxy-methamphetamine. A systematic review and meta-analysis of randomized controlled trials
Authors: Fluyau Dimy , Kailasam Vasanth Kattalai , Revadigar Neelambika 
Published: June 2024

AbstractBackgroundHallucinogens attract research as alternatives to the commonly used medications to treat major depressive and anxiety disorders.AimsAssess hallucinogens’ efficacy for managing depressive and anxiety symptoms and evaluate their safety profiles.MethodIn five databases, we searched for randomized controlled trials of hallucinogens targeting depressive and anxiety symptoms. We performed a meta-analysis using a random effects model when data permitted it. The protocol of the review is registered in PROSPERO; CRD42022341325.ResultsPsilocybin produced a rapid and sustained reduction in depressive and anxiety symptoms in patients with major depressive disorder, severe, and in patients with life-threatening cancer. A decrease in depressive symptoms was observed with 3, 4-methylenedioxymethamphetamine (MDMA), primarily in patients with life-threatening cancer, autism spectrum disorder, and post-traumatic stress disorder. MDMA reduced social anxiety symptoms. However, MDMA’s effect size was either negligible or negative for anxiety symptoms overall. Ayahuasca reduced depressive symptoms in individuals with treatment-resistant major depressive and personality disorders. Lysergic acid diethylamide (LSD) reduced anxiety symptoms in individuals with life-threatening cancer.Psilocybin’s adverse effects were noticeable for elevated blood pressure, headaches, and panic attacks. For MDMA, elevated blood pressure, headaches, panic attacks, and feeling cold were noticeable.ConclusionsPsilocybin, MDMA, ayahuasca, and LSD appear to have the potential to reduce depressive and anxiety symptoms. Adverse effects are noticed. Rigorous randomized controlled studies with larger sample sizes utilizing outcome measures instruments with better reliability and validity are warranted.
Feedback Control of Neuronal Excitability and Epileptiform Bursting using a Photocaged Adenosine A1Agonist
Authors: Craey Erine , Van Calenbergh Serge , Spanoghe Jeroen , Vergaelen Marijke , Larsen Lars E. , Carrette Evelien , Delbeke Jean , Vonck Kristl , Boon Paul , Wadman Wytse J. , Raedt Robrecht 
Published: June 2024

AbstractAdenosine is a potent regulator of neurotransmission and neuronal excitability through activation of Giprotein-coupled adenosine A1receptors (A1Rs). It has gained interest as a potential anticonvulsant due to its endogenous involvement in ending ongoing seizure activity.A recently developed coumarin-caged derivative of the A1R agonist N6-cyclopentyl-adenosine (CPA), cCPA, was used for photo-uncaging of CPA with millisecond flashes of 405 nm light. At population level, CPA reduces Schaffer Collateral stimulated extracellular dendritic field potentials (FPs) in the CA1 region of the hippocampus with an ED50of 44.1±2.8 nM and a Hill coefficient of 3. Response onset is CPA dependent and takes less than seconds, while recovery is CPA independent with a time constant of around 20 minutes.A closed-loop feedback system used the amplitude of evoked dendritic FPs to photorelease CPA and was able to control FP amplitude to user defined levels between 10% and 90% of baseline level. In the acute elevated potassium model of epilepsy raising extracellular K+to 8.5 mM enhances neuronal excitability and induces regularly occurring epileptiform bursts, but FPs evoked with low intensity could still continuously monitor excitability without interfering with bursting.In this model the closed-loop system that controlled CPA release, was able to suppress epileptiform bursting, while maintaining an acceptable level of functional neurotransmission. Including in the control algorithm a second parameter that combined population spike amplitude and number of population spikes, enabled the system to automatically find a level of functional neurotransmission that was just below the threshold for multiple spiking and epileptiform bursting.The combination of photopharmacological adenosinergic modulation with real-time FP monitoring provides a first step towards closed-loop precision treatment for diseases related to neuronal hyperexcitability such as epilepsy.
Strand asymmetry in the repair of replication dependent double-strand breaks
Authors: Kimble Michael T , Sane Aakanksha , Reid Robert JD , Johnson Matthew J , Rothstein Rodney , Symington Lorraine S 
Published: June 2024

SUMMARYSingle-strand breaks (SSBs) are one of the most common endogenous lesions and have the potential to give rise to cytotoxic double-strand breaks (DSBs) during DNA replication. To investigate the mechanism of replication fork collapse at SSBs and subsequent repair, we employed Cas9 nickase (nCas9) to generate site and strand-specific nicks in the budding yeast genome. We show that nCas9-induced nicks are converted to mostly double-ended DSBs during S-phase. We find that repair of replication-dependent DSBs requires homologous recombination (HR) and is independent of canonical non-homologous end joining. Consistent with a strong bias to repair these lesions using a sister chromatid template, we observe minimal induction of inter-chromosomal HR by nCas9. Using nCas9 and a gRNA to nick either the leading or lagging strand template, we carried out a genome-wide screen to identify factors necessary for the repair of replication-dependent DSBs. All the core HR genes were recovered in the screen with both gRNAs, but we recovered components of the replication-coupled nucleosome assembly (RCNA) pathway with only the gRNA targeting the leading strand template. By use of additional gRNAs, we find that the RCNA pathway is especially important to repair a leading strand fork collapse.
The GTPase-activating protein CG42795 is a potent neuronal regulator of ageing in Drosophila melanogaster
Authors: Falcsik Gergő , Kersztes Fanni , Balog Tünde Mónika , Kovács Tibor 
Published: June 2024

AbstractMacroautophagy (hereafter referred to as autophagy) is an important self-renewal process in our cells, whereby potentially harmful cellular components are encapsulated within a double-membrane autophagosome and subsequently fused with a lysosome for degradation by acidic hydrolases. This lysosomal degradation process is essential for maintaining cellular homeostasis. Dysfunctional autophagy can lead to the accumulation of cytotoxic protein forms that contribute to the onset of age-related diseases. However, it has been proven that autophagy activity declines with age so it is therefore particularly important to stimulate autophagy during the lifespan of post-mitotic cells, such as neurons, where cell division is not a possibility in order to replace dead cells. Our research group aims to find new autophagy activation sites to stimulate the efficiency of acid degradation in neurons during ageing. One possibility is the stimulation of membrane fusion events, which are necessary for autophagic degradation, through the activation of small GTPase enzymes. Our previous results have shown that neuron-specific overexpression of the activated form of the Rab2 small GTPase has autophagy and lifespan-enhancing effects. In the present experiment, we used an RNA interference screen to investigate whether silencing 12 GTPase-activating proteins (GAPs) belonging to the TBC1 domain family can enhance Rab2 activation in theDrosophilanervous system. Several of the GAPs studied increased the number of Rab2-positive structures, 5 of which were selected for further screening. Our results suggest that neuronal silencing ofCG42795exerts an effect on autophagy, with the capacity to enhance the locomotor ability of animals and prolong lifespan. Our research has shown that the study of GAPs could be a promising new avenue of research for ageing researchers. Further analysis of CG42795 could lead to the development of potential autophagy activators.
Defining Structure-Function Relationships of Amphiphilic Excipients Enables Rational Design of Ultra-Stable Biopharmaceuticals
Authors: Prossnitz Alexander N. , Nguyen Leslee T. , Eckman Noah , Borkar Suraj , Tetef Samantha , Auzten Anton A. A. , Fuller Gerald G. , Appel Eric A. 
Published: June 2024

SummaryBiopharmaceuticals are the fastest growing class of drugs in the healthcare industry, but their global reach is severely limited by their propensity for rapid aggregation. Currently, surfactant excipients such as polysorbates and poloxamers are used to prevent protein aggregation, which significantly extends shelf-life. Unfortunately, these excipients are themselves unstable, oxidizing rapidly into 100s of distinct compounds, some of which cause severe adverse events in patients. Here, we leverage the highly stable, well-defined, and modular nature of amphiphilic polyacrylamide-derived excipients to isolate the key mechanisms responsible for excipient-mediated protein stabilization. With a library of compositionally identical but structurally distinct amphiphilic copolymers, we quantify multiple relationships between polymer properties and fundamental phenomena to rationally design new ultra-stable surfactant excipients, increasing the stability of a notoriously unstable biopharmaceutical, monomeric insulin, by an order of magnitude. This comprehensive and generalizable understanding of excipient structure-function relationships represents a paradigm shift for the formulation of biopharmaceuticals, moving away from trial-and-error screening approaches towards rational design.TOC Graphic
The evolutionary dynamics of plant mating systems: how bias for studying ‘interesting’ plant reproductive systems could backfire
Authors: Meyer Elena M. , Galloway Laura F. , Eckert Andrew J. 
Published: June 2024

AbstractBackground and AimsAn “abominable mystery”: angiosperm sexual systems have been a source of both interest and frustration for the botanical community since Darwin. The evolutionary stability, overall frequency, and distribution of self-fertilization and mixed-mating systems have been explored in a variety of studies. However, there has been no recent study which directly addresses our knowledge of mating systems across families, the adequacy of existing data, or the potential for biases.ScopeHere we present an updated dataset of mating systems across flowering plants covering 6,781 species and 212 families based on a synthesis of existing reviews and an original literature review using Web of Science. We assess the adequacy of this data by evaluating for bias indicating enrichment of certain families or sexual systems.Key ResultsWe find that the vast majority of our data on mating systems comes from a small number of disproportionally sampled families, and that families with significant proportions of dioecious or monoecious species are much more likely to be undersampled.ConclusionsOur results show that the frequency of selfing in angiosperms is overestimated, possibly due to increased research interest in selfing and mixed-mating systems. This suggests that systematic study bias may mean we know less about this vital facet of plant life than we think.
Morphology, biology and phylogeny ofXenos gadagkarisp.nov. (Strepsiptera: Xenidae): an endoparasite ofPolistes wattii(Hymenoptera: Vespidae)
Authors: Nain Deepak , Rana Anjali , Raychoudhury Rhitoban , Sen Ruchira 
Published: June 2024

AbstractWe report the morphology, biology and phylogeny of a new strepsipteran endoparasitic species:Xenos gadagkari,from the primitively eusocial waspPolistes wattiifrom Punjab, India. We report a high abundance of the endoparasite inP. wattiipopulation of our study area and provide the morphological description of adult males, male puparium and neotenic females. This species is sufficiently different from otherXenos spreported from various social wasps and therefore, constitutes a new species,X. gadagkariSen and Nain, sp. nov. Although there are a few reports of Strepsipteran parasites from India, this is the first confirmational report with a thorough description of aXenosspecies from the Indian population ofP. wattiiand underscores the need for further investigation of the diversity and distribution of this group in India. Our findings contribute to the growing body of knowledge onXenosand provide a foundation for future behavioural, microbial and molecular studies on this enigmatic group of insects.
The non-canonical thioreductase TMX2 is essential for neuronal survival during embryonic brain development
Authors: Dekker Jordy , Lam Wendy , van der Linde Herma C. , Ophorst Floris , de Konink Charlotte , Schot Rachel , Kremers Gert-Jan , Sanderson Leslie E. , Berdowski Woutje M. , van Woerden Geeske M. , Mancini Grazia M.S. , van Ham Tjakko J. 
Published: June 2024

AbstractBiallelic variants in thioredoxin-related transmembrane 2 protein (TMX2) can cause a brain malformation of cortical development (MCD), characterized by primary microcephaly, polymicrogyria and pachygyria by an unknown mechanism. To better understand and visualize how TMX2 loss disrupts brain developmentin vivowe investigated the function of TMX2, using the zebrafish embryo as a model system. We generated zebrafish deficient forTMX2orthologtmx2b, which during the first 2 days post fertilization (dpf) showed normal behavioral activity and brain developmental hallmarks. From 3 dpf onwards however,tmx2bmutants failed to exhibit locomotor activity, which was accompanied by cell death primarily in the brain, but not in other organs or in the spinal cord. Strikingly, cell death intmx2bmutants occurs specifically in newborn neurons within a ∼1.5-hour timeframe, whereas neuronal progenitor and other glial cells are preserved, and could be suppressed by inhibiting neuronal activity.In vivoGCaMP6s calcium imaging showed a persistent ∼2-fold increase in calcium in neurons after the onset of cell death. This suggests that calcium homeostasis underlies thetmx2bmutant brain phenotype. Altogether, our results indicate that TMX2 is an evolutionary conserved, protective regulator essential specifically for newborn neurons to survive after their differentiation in the vertebrate embryonic brain.
Analysis of high-molecular-weight proteins using MALDI-TOF MS and Machine Learning for the differentiation of clinically relevantClostridioides difficileribotypes
Authors: Candela Ana , Rodriguez-Temporal David , Blázquez-Sánchez Mario , Arroyo Manuel J. , Marín Mercedes , Alcalá Luis , Bou Germán , Rodríguez-Sánchez Belén , Oviaño Marina 
Published: June 2024

AbstractClostridioides difficileis the main cause of antibiotic related diarrhea and some ribotypes (RT), such as RT027, RT181 or RT078, are considered high risk clones. A fast and reliable approach forC. difficileribotyping is needed for a correct clinical approach. This study analyses high-molecular-weight proteins forC. difficileribotyping with MALDI-TOF MS. Sixty-nine isolates representative of the most common ribotypes in Europe were analyzed in the 17,000-65,000m/zregion and classified into 4 categories (RT027, RT181, RT078 and ‘Other RTs’). Five supervised Machine Learning algorithms were tested for this purpose: K-Nearest Neighbors, Support Vector Machine, Partial Least Squares-Discriminant Analysis, Random Forest and Light-Gradient Boosting Machine. All algorithms yielded cross-validation results >70%, being RF and Light-GBM the best performing, with 88% of agreement. Area under the ROC curve of these two algorithms was >0.9. RT078 was correctly classified with 100% accuracy and isolates from the RT181 category could not be differentiated from RT027.
Effects of Resistance Training Combined with a Ketogenic Diet: A Systematic Review and Meta-Analysis
Authors: Sinott Luis Roberto , da Silva Clédia Silveira Flores , Scheer Alice Künzgen , Atrib Amanda Barbosa , Scheneider Augusto , Barros Carlos Castilho 
Published: June 2024

AbstractWeight loss treatments require adherence to physical exercise and diet. Restrictive diets have been proposed for obesity treatment, including a ketogenic diet that are high in lipids, moderate in proteins, and low in carbohydrates. In recent years, there has been criticism of this diet because of the reduction in fat-free mass and, consequently, a reduction in basal energy expenditure, which is considered negative in obesity treatment. However, resistance training is known to promote skeletal muscle hypertrophy. The hypothesis for this review was: “Resistance training is sufficient to maintain lean mass during diets that cause ketosis.” Despite the slight reduction in lean mass identified in the meta-analysis, some authors reported no loss in physical performance. Others suggested that this difference in lean mass is associated with water loss in the participants, which aligns with a few studies that reported a final phase with carbohydrate reintroduction into the diet. Our results indicated physical exercise was an important tool for maintaining lean mass in individuals who consumed carbohydrate-restricted diets that cause ketosis.
Effects of Cannabidiol on Social Relating, Anxiety, and Parental Stress in Autistic Children: A Randomised Controlled Crossover Trial
Authors: Parrella Nina-Francesca , Hill Aron T. , Enticott Peter G. , Botha Tanita , Catchlove Sarah , Downey Luke , Ford Talitha C. 
Published: June 2024

AbstractCannabidiol (CBD), a non-intoxicating compound derived from the cannabis plant, has garnered increasing attention as a potential pharmacological therapeutic for autistic children. We conducted a randomised, double-blind, placebo-controlled, crossover trial to understand whether oral CBD oil can improve outcomes for this population, with a primary focus on social relating outcomes, along with anxiety and parental stress. A total of 29 children (18 male), aged 5 to 12 years (M = 9.62 years, SD = 2.05), diagnosed with autism spectrum disorder, completed the study. Participants received weight-based dosing of CBD oil (10 mg/kg/day) or placebo oil over two 12-week intervention periods (crossover), separated by an 8-week washout period. Outcome measures included the Social Responsiveness Scale-2 (SRS-2; primary outcome), PROMIS Social Relating, Anxiety, and Sleep, Developmental Behaviour Checklist-2 (DBC-2), Vineland 3, and Autism Parenting Stress Index (APSI; secondary outcomes). There was no significant effect observed for the primary outcome measure (SRS-2) for CBD oil relative to placebo oil after 12 weeks, (p=.125). Significant improvements were observed in secondary measures of social functioning (PROMIS-Social, DBC-2 Social Relating;p<.05) and anxiety symptoms (PROMIS Anxiety, DBC-2 Anxiety;p<.05), while there was also a reduction in parental stress (p<.05). Safety and tolerability data indicated that two children experienced gastrointestinal discomfort while taking CBD. This pilot trial represents preliminary evidence for the potential therapeutic effects of CBD in autism. While further research is needed to confirm and extend these findings, the results suggest that CBD may be a promising intervention for addressing one of autism’s core symptoms: social relating. Future studies with larger sample sizes are needed to fully evaluate the efficacy and safety of CBD for autistic children.
Overview of effects of motor learning strategies in neurological and geriatric populations: a systematic mapping review
Authors: Jie Li-Juan , Kleynen Melanie , Rothuizen Guus , Kal Elmar , Rothgangel Andreas , Braun Susy 
Published: June 2024

AbstractIntroductionMotor learning plays a central role in neurological and geriatric rehabilitation. The wide range of motor learning strategies and increase in evidence can make it difficult to make informed decisions about the use of motor learning strategies in practice. This review’s aim was to provide a broad overview of the current state of research regarding the effects of seven commonly used motor learning strategies to improve functional tasks within older neurological and geriatric populations.MethodA systematic mapping review of randomised controlled trials was conducted regarding the effectiveness of seven motor learning strategies – errorless learning, analogy learning, observational learning, trial-and-error learning, dual-task learning, discovery learning, and movement imagery – within the geriatric and neurological population. PubMed, CINAHL, and Embase databases were searched. The Risk of Bias 2 tool was used to assess bias; additionally, papers underwent screening for sample size justification.ResultsEighty-seven articles were included. Identified articles regarding the effects of the targeted motor learning strategies started around the year 2000 and mainly emerged since 2010. Eight different populations were included, e.g. Parkinson’s, and stroke. Studies were not equally balanced across the motor learning strategies or target groups and overall showed a moderate to high risk of bias. Positive trends regarding effects were observed for dual-tasking, observational learning and movement imagery.ConclusionsThe findings show a skewed distribution of studies across motor learning interventions, which have been researched within a variety of populations. Methodological shortcomings make it difficult to draw firm conclusions regarding the effectiveness of motor learning strategies. Future researchers are strongly advised to follow guidelines that aid in maintaining methodological quality. Moreover, alternative designs fitting the complex practice situation should be considered.