bluuebunny/crossref_metadata_2025_split huggingface dataset:
Extracellular matrix provides an optimal niche for the maintenance and propagation of mesenchymal stem cells
Authors: Chen Xiao‐Dong 
Published: March 2010

AbstractRelatively little is known about the cellular and molecular mechanisms underlying the control of mesenchymal stem cell (MSC) proliferation, differentiation, and survival. This presents difficulties in following and characterizing cells along the lineage because of our inability to isolate and obtain a sufficient number of homogeneous MSCs using current culture systems for in vitro expansion. Adjusting the cellular machinery to allow greater proliferation can lead to other unwanted outcomes, such as unmanageable precancerous changes, or differentiation down an undesired pathway. Recently, it has become increasingly evident that the extracellular matrix (ECM) is an important component of the cellular niche in a tissue, supplying critical biochemical and physical signals to initiate and sustain cellular functions. Indeed, it is very doubtful that the intricate and highly ordered nature of the ECM could be reproduced with synthetic or purified components. This review cites evidence that supports an alternative approach for maintenance of MSCs by simulating in vitro the bone marrow ECM, where MSCs reside in vivo, and discusses the potential mechanisms whereby the ECM regulates the exposure of cells to growth factors that subsequently control MSC replication and differentiation, and also how the ECM provides unique cues that govern the lineage specification and differentiation of MSCs. Birth Defects Research (Part C) 90:45–54, 2010. © 2010 Wiley‐Liss, Inc.
The role of mechanical signals in regulating chondrogenesis and osteogenesis of mesenchymal stem cells
Authors: Kelly Daniel J. , Jacobs Christopher R. 
Published: March 2010

AbstractIt is becoming increasingly clear that mesenchymal stem cell (MSC) differentiation is regulated by mechanical signals. Mechanical forces generated intrinsically within the cell in response to its extracellular environment, and extrinsic mechanical signals imposed upon the cell by the extracellular environment, play a central role in determining MSC fate. This article reviews chondrogenesis and osteogenesis during skeletogenesis, and then considers the role of mechanics in regulating limb development and regenerative events such as fracture repair. However, observing skeletal changes under altered loading conditions can only partially explain the role of mechanics in controlling MSC differentiation. Increasingly, understanding how epigenetic factors, such as the mechanical environment, regulate stem cell fate is undertaken using tightly controlled in vitro models. Factors such as bioengineered surfaces, substrates, and bioreactor systems are used to control the mechanical forces imposed upon, and generated within, MSCs. From these studies, a clearer picture of how osteogenesis and chondrogenesis of MSCs is regulated by mechanical signals is beginning to emerge. Understanding the response of MSCs to such regulatory factors is a key step towards understanding their role in development, disease and regeneration. Birth Defects Research (Part C) 90:75–85, 2010. © 2010 Wiley‐Liss, Inc.
The effects of prompting, fading, and differential reinforcement on vocal mands in non‐verbal preschool children with autism spectrum disorders
Authors: Thomas Benjamin R. , Lafasakis Michael , Sturmey Peter 
Published: March 2010

AbstractThere are few procedures to teach non‐vocal children vocal mands. This study evaluated the effects of prompting, fading, and differential reinforcement on eye contact, pointing, vocal approximations, independent requests and immature mands in three children with Autism Spectrum Disorders who in baseline emitted almost no independent vocal mands. This procedure resulted in a large and socially valid increase in independent vocal mands, other appropriate responses and near elimination of immature mands. Copyright © 2010 John Wiley & Sons, Ltd.
Developing and assessing stimulus control based on establishing operations during mand training using representative objects
Authors: Gutierrez Anibal , Vollmer Timothy R. , Samaha Andrew L. 
Published: March 2010

AbstractThe acquisition of verbal behavior can be difficult for some individuals with severe developmental disabilities. For two individuals who previously did not acquire picture exchange communication despite extensive efforts, we evaluated the effectiveness of using representative objects to teach mands for preferred items. In addition, we replicated and extended the findings of our previous research manipulating motivating operations (MO) for functional discriminated mands. Two individuals with developmental disabilities participated in a training procedure designed to teach two separate mands for two separate preferred items. Participants were taught to mand (e.g., for chips) using representative objects (e.g., empty bag of chips). Following training, the MO manipulation was used to assess for discriminated manding. This manipulation involved providing free access to one of the preferred items, such that there should be no motivation to ask for it, while motivation to ask for the other item remained in place. Results showed that both participants acquired at least a degree of discriminated manding using representative objects. At a minimum, a method was established to test complex discrimina‐ tions for preferred objects. Although we previously used this procedure in teaching picture card exchange discriminations, the current research extended previous research to individuals who were previously unresponsive to training and by using representative objects. Copyright © 2010 John Wiley & Sons, Ltd.
Effects of textual response prompts for adolescents in a substance abuse treatment program
Authors: Taylor Sarah Ann , Anderson Kerrie , Mudford Oliver C. 
Published: March 2010

AbstractAdolescents in a drug and alcohol treatment facility had behavioral deficits in having essential items ready for organized group adventure activities. Checklists (i.e., textual response prompts) were introduced. The data from five participants showed increases in the percentage of required items ready. Generalization was demonstrated across checklists for different activities, across staff and peer leaders, and a different day of the week. Performance was also maintained when the checklist was removed. It was concluded that the use of checklists can be a reliable and efficient strategy to improve performance in adolescents with a substance abuse history. Copyright © 2010 John Wiley & Sons, Ltd.
Study on the interaction between human serum albumin and a novel bioactive acridine derivative using optical spectroscopy
Authors: Chen Liangbi , Wu Menghui , Lin Xucong , Xie Zenghong 
Published: March 2010

AbstractThe interaction of a novel bioactive agent N‐{[N‐(2‐dimethylamino) ethyl] acridine‐4‐carboxamide}‐α‐alanine [N‐(ACR‐4‐CA)‐α‐ALA] with human serum albumin (HSA) was investigated by fluorescence spectroscopy, UV–vis absorption and circular dichroism spectrophotometric techniques under simulative physiological conditions. The fluorescence quenching of HSA by addition of N‐(ACR‐4‐CA)‐α‐ALA is due to static quenching and hydrogen bonding. Moreover, hydrophobic interactions play a role in the binding of N‐(ACR‐4‐CA)‐α‐ALA to HSA as well. The number of binding sites, n, and the binding constant values, KA, were noted to be 0.88 and 3.4 × 104 L mol−1 for N‐(ACR‐4‐CA)‐α‐ALA at 293 K. The binding distances and the energy transfer efficiency between N‐(ACR‐4‐CA)‐α‐ALA and protein were determined. The negative value of enthalpy change and positive value of entropy change in the present study indicated that both hydrogen bonding and hydrophobic forces played a major role in the binding of N‐(ACR‐4‐CA)‐α‐ALA to HSA. Copyright © 2010 John Wiley & Sons, Ltd.
Electrochemiluminescence of palmatine being oxidized by electrogenerated hydroxyl radical and its analytical application
Authors: Liang Yao‐Dong , Yu Chun‐Xia , Song Jun‐Feng 
Published: March 2010

AbstractA strong electrochemiluminescence (ECL) of palmatine in NaOH medium was observed at a vaseline‐impregnated graphite anode. The ECL production could be described as follows: hydroxyl radical (OH•) was generated via the oxidation of hydroxyl group (OH‐) in NaOH medium, and the formed OH• subsequently oxidized palmatine base converted from palmatine in NaOH medium to the excited state oxypalmatine (oxypalmatine*). As the oxypalmatine* went back to its ground state, a stronger chemiluminescence was produced. Based on the ECL of palmatine, an ECL method for the determination of palmatine was proposed. An ECL signal of palmatine in NaOH solution was obtained by applying direct current of 15 mA to the vaseline‐impregnated graphite anode. The ECL intensity was rectilinear with palmatine concentration in the range of 8.0 × 10−7 to 2.0 × 10−5 mol l−1 and the limit of detection (signal‐to‐noise = 3) was 3 × 10−7 mol l−1. The proposed method was applied to the determination of palmatine in pharmaceutical preparations. Copyright © 2010 John Wiley & Sons, Ltd.
The configuration, solvatochromism and metallo‐responses of two novel cyano‐containing oligo(phenylene‐vinylene) derivatives
Authors: Lv Jing , Yin Xiaodong , Zheng Haiyan , Li Yongjun , Li Yuliang , Zhu Daoben 
Published: March 2010

AbstractTwo novel cyano‐containing oligo(phenylenevinylene) (OPV) derivatives have been designed and synthesized. Photophysical and sensing properties of the two compounds were studied. Such studies reveal the intramolecular charge transfer process between cyano groups and OPV core. The results showed that the alkyl difference of substituted OPV leads to the changes of molecular configuration and metallo‐response of two compounds. Copyright © 2010 John Wiley & Sons, Ltd.
Semi‐micro flow injection analysis method for evaluation of quenching effect of health foods or food additive antioxidants on peroxynitrite
Authors: Wada Mitsuhiro , Kira Megumi , Kido Hirotsugu , Ikeda Rie , Kuroda Naotaka , Nishigaki Toshiaki , Nakashima Kenichiro 
Published: March 2010

AbstractA semi‐micro flow injection analysis (SMFIA) method for evaluation of quenching effect of food additive antioxidants or health foods on peroxynitrite (ONOO‐) is described. The injected sample was carried with phosphate buffer containing NaNO2, mixed with a trigger solution to generate ONOO‐ and then detected CL generated after mixing with luminol solution. Selective chemiluminescence caused by ONOO‐ in this generation system was confirmed by catalase treatment. Ascorbic acid (ASA), Trolox and ascorbyl palmitate (ASP) were used as food additive antioxidants. EC50 values of ASA, Trolox and ASP were 2.6, 6.4 and 43 µg/mL, respectively. The amount of reagents required for an assay by this SMFIA system could reduce the time by a third compared with the conventional method previously reported. Furthermore, as an application of the proposed method, the quenching effect of commercially available Noni (Morinda citrifolia) juices was evaluated. Copyright © 2010 John Wiley & Sons, Ltd.
Bariatric surgery reduces mortality in Swedish men
Authors: Marsk R , Näslund E , Freedman J , Tynelius P , Rasmussen F 
Published: March 2010

AbstractBackgroundMortality is lower in obese patients who have undergone surgery for obesity than in those who have not. The majority of patients in these studies have been women. Perioperative mortality is known to be higher among men, and this may counterbalance the survival advantage seen after surgery. This cohort study compared mortality among operated obese patients, non-operated obese patients and a general control cohort of men.MethodsThe study was based on record linkage between Swedish registries. An operated obese, a non-operated obese and a general control cohort were created. The two non-operated cohorts were assigned pseudosurgery dates. Data regarding preoperative and postoperative morbidity were collected, as well as mortality data.ResultsHazard ratios were calculated for mortality between the cohorts adjusting for preoperative morbidity and age. Comparison of all-cause mortality for the obese surgical and non-surgical cohorts gave an adjusted mortality risk of 0·7 (95 per cent confidence interval (c.i.) 0·5 to 1·0) (P = 0·039); the adjusted mortality risk was 1·5 (95 per cent c.i. 1·1 to 2·0) (P = 0·011) when the obese surgical cohort was compared with the general control cohort.ConclusionBariatric surgery reduces overall mortality in obese men.
Intraoperative near-infrared image-guided surgery for peritoneal carcinomatosis in a preclinical experimental model
Authors: Keramidas M , Josserand V , Righini C A , Wenk C , Faure C , Coll J L 
Published: March 2010

Abstract

Background
This study compared the quality of surgery performed under conventional light with near-infrared (NIR) image-guided surgery using a tumour-targeting probe and a portable clinical grade imaging device in a mouse model of peritoneal carcinomatosis.

Methods
Peritoneal carcinomatosis was induced by injection of luciferase-positive tumour cells, leading to the formation of small nodules in the peritoneal cavity. One day after intravenous injection of RAFT-c(RGDfK)4-Alexa Fluor® 700, a fluorescent tumour-targeting probe, the surgeon operated using the Fluobeam®, a portable device that illuminated the mouse with NIR light and allowed NIR vision. The quality of the surgery was evaluated using bioluminescence, a highly sensitive method that detected the remaining tumour cells, and operating time was measured.

Results
Under normal light, the surgeon detected and removed a mean(s.d.) of only 50·6(2·3) per cent of the nodules that were visible under NIR light. The duration of surgery was reduced from 19·5(3·3) min under normal light to 14·0(2·6) min when NIR light was used (P = 0·025). The sensitivity of the NIR system allowed the detection of nodules containing as few as 227 tumour cells.

Conclusion
NIR image-guided surgery improved the quality of surgery for peritoneal carcinomatosis by doubling the number of nodules detected and significantly reducing the duration of surgery.
Surgical management of primary retroperitoneal sarcoma
Authors: Strauss D C , Hayes A J , Thway K , Moskovic E C , Fisher C , Thomas J M 
Published: March 2010

Abstract

Background
Local recurrence after surgical resection is the main cause of disease-related mortality in patients with primary retroperitoneal sarcoma (RPS). This study analysed predictors of local recurrence and disease-specific survival.

Methods
A prospective database was reviewed to identify patients who underwent surgery for primary RPS between 1990 and 2009. Patient demographics, operative outcomes and tumour variables were correlated with local recurrence and disease-specific survival. Multivariable analysis was performed to evaluate predictors for local recurrence and disease-free survival.

Results
Macroscopic clearance was achieved in 170 of 200 patients. The median weight of tumours was 4·0 kg and median maximum diameter 27 cm. Resection of adjacent organs was required in 126 patients. The postoperative mortality rate was 3·0 per cent. Seventy-five patients developed local recurrence during follow-up. At 5 years the local recurrence-free survival rate was 54·6 per cent and the disease-specific survival rate 68·6 per cent. Inability to obtain macroscopic clearance at resection and high-grade tumours were significant predictors for local recurrence and disease-specific survival.

Conclusion
Complete macroscopic excision should be the goal of surgical resection. Ability to resect a RPS completely and tumour grade are the most important predictors of local recurrence and overall survival.
Endoscopic resection of early gastric cancer treated by guideline and expanded National Cancer Centre criteria
Authors: Gotoda T , Iwasaki M , Kusano C , Seewald S , Oda I 
Published: March 2010

Abstract

Background
Criteria for endoscopic resection in patients with early gastric cancer (EGC) have been expanded recently by the National Cancer Centre (NCC). This study compared long-term outcomes in patients with EGC who underwent endoscopic treatment according to guideline criteria with those treated according to expanded criteria.

Methods
Baseline and outcome data from patients undergoing curative endoscopic resection for EGC between January 1999 and December 2005 were collected from electronic medical records. Survival time hazard ratios and 95 per cent confidence intervals were calculated using the Cox proportional hazards model.

Results
Of 1485 patients who had a curative resection, 635 (42·8 per cent) underwent resection according to traditional criteria and 625 (42·1 per cent) according to expanded criteria. There was no significant difference in overall survival between the groups.

Conclusion
Patients who have treatment following the expanded criteria have similar long-term survival and outcomes to those treated according to guideline criteria.
Developing object‐based learning environment to promote learners' motivation for learning digital systems
Authors: Siong Hoe Lau , Woods Peter C. 
Published: March 2010

AbstractThis article aims at sharing the authors' experiences with object‐based learning environment. The purpose of this study is to assess the learners' perceptions and usage of learning objects in Digital Systems course. Overall, learning objects are good alternative learning strategy, which enables learners to take more control over their own learning. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 18: 640–650, 2010; View this article online at wileyonlinelibrary.com; DOI 10.1002/cae20268
Teaching mechatronics: An innovative group project‐based approach
Authors: Shyr Wen‐Jye 
Published: March 2010

AbstractThis study presents an innovative group project‐based approach to teaching mechatronics. Mechatronics is a complex, highly technical and multidisciplinary field involving the design and manufacture of integrated products. Mechatronics course at the undergraduate level is rapidly increasing across the world. Most courses require student teams to design a product. The complexity of student projects can make administration of mechatronics courses extremely difficult. Students develop both practical and theoretical understanding of mechatronics while working on group projects. They also develop the interpersonal and communication skills needed to work in a multi‐disciplinary field. This study describes a group project‐based approach for enabling teams of students to complete mechatronics projects. A set of heuristic guidelines is also proposed. At the National Changhua University of Education in the Department of Industrial Education and Technology, this approach has yielded high student satisfaction and achievement. © 2010 Wiley Periodicals, Inc. Comput Appl Eng Educ 20: 93–102, 2012
Successful use of the five‐in‐six system with IVUS guided transradial renal artery stenting
Authors: Abdou Sayed M. , Wu Chiung‐Jen 
Published: March 2010

AbstractIn transradial renal intervention, the long distance between the vascular access and the target lesion represents a major issue. The 100 cm guiding catheter (GC) may be too short to engage the renal artery particularly in tall patients or with excessive aortic turtousity. On the other hand, with the use of 125 cm long GC, only few premounted dedicated renal stents can be used. The shaft of the stent delivery system must be 140 cm or more to be compatible with the 125 GC. However, in many renal interventions, only few more centimeters are required to give the GC the sufficient working length needed for proper engagement and back up support. For this purpose, we used the Terumo “five‐in‐six” system to provide the necessary extra length through the protruding part of the 5 Fr GC out of the tip of the 6 Fr GC. With the super soft distal segment of the 5 FR GC deeply intubated into the right renal artery, we had the sufficient backup support to do renal stenting with IVUS guidance. This case illustrates that the “five‐in‐six” system can be an alternative strategy whenever extra length or strong backup support is needed during transradial renal artery intervention. © 2011 Wiley‐Liss, Inc.
Transcatheter occlusion of the left atrial appendage—Experimental testing of a new amplatzer device
Authors: Bass John L. 
Published: March 2010

AbstractObjectives: This study assessed the feasibility and efficacy of implanting a new nitinol device in the left atrial appendage (LAA) in a canine model. Background: Left atrial thrombus occurs in the LAA in 90% of humans with nonvalvular atrial fibrillation (AF). Methods: The Amplatzer Left Atrial Appendage Occluder Device now known as the Amplatzer Cardiac Plug (Amplatzer Medical LLC, Plymouth MN) is a new transcatheter Nitinol device containing polyester fabric designed to seal the LAA. The device was implanted in 10 canines with immediate, ∼30 and ∼90‐day follow‐up by echocardiography, angiography, and final pathological examination. Results: The device was successfully implanted in all animals and found to be retrievable and repositionable. There was complete occlusion of the LAA in all cases without embolization. There was no thrombus formation on the device and no pericardial effusion or impingement on the circumflex coronary artery, mitral valve, or left pulmonary vein. A single instance of tiny left atrial mural thrombus at device end screw contact was attributed to the small canine left atrium. Conclusions: The success of this animal study confirms safety and feasibility of the Amplatzer Cardiac Plug. Human trials are warranted. © 2010 Wiley‐Liss, Inc.
Efficacy of everolimus eluting stent implantation in patients with calcified coronary culprit lesions: Two‐year angiographic and three‐year clinical results from the SPIRIT II study
Authors: Onuma Yoshinobu , Tanimoto Shuzou , Ruygrok Peter , Neuzner Jörg , Piek Jan J. , Seth Ashok , Schofer Joachim J. , Richardt Gert , Wiemer Marcus , Carrié Didier , Thuesen Leif , Dorange Cecile , Miquel‐Hebert Karine , Veldhof Susan , Serruys Patrick W. 
Published: March 2010

AbstractBackground: Little is known about the impact of treatment with drug‐eluting stents (DES) on calcified coronary lesions. This analysis sought to assess the safety and efficacy of the XIENCE V everolimus‐eluting stent (EES) in patients with calcified or noncalcified culprit lesions. Methods: The study population consisted of 212 patients with 247 lesions, who were treated with EES alone. Target lesions were angiographically classified as none/mild, moderate, or severe grades of calcification. The population was divided into two groups: those with at least one target lesion moderately or severely calcified (the calcified group: 68 patients with 75 calcified lesions) and those with all target lesions having mild or no calcification (the noncalcified group: 144 patients). Six‐month and 2‐year angiographic follow‐up and clinical follow‐up up to 3 years were completed. Results: The baseline characteristics were not significantly different between both groups. When compared with the noncalcified group, the calcified group had significantly higher rates of 6‐month in‐stent angiographic binary restenosis (ABR, 4.3% vs. 0%, P = 0.03) and ischemia‐driven target lesion revascularization (ID‐TLR, 5.9% vs. 0%, P = 0.01), resulting in numerically higher major cardiac adverse events (MACE, 5.9% vs. 1.4%, P = 0.09). At 2 years, when compared with the noncalcified group, the calcified group presented higher in‐stent ABR (7.4% vs. 0%, P = 0.08) and ID‐TLR (7.8% vs. 1.5%, P = 0.03), resulting in numerically higher MACE (10.9% vs. 4.4%, P = 0.12). At 3 years, ID‐TLR tended to be higher in the calcified group than in the noncalcified group (8.6% vs. 2.4%, P = 0.11), resulting in numerically higher MACE (12.1% vs. 4.7%, P = 0.12). Conclusions: The MACE rates in patients treated with EES for calcified lesions were higher than in those for noncalcified lesions, but remained lower than the results of previously reported stent studies. EES implantation in patients with calcified culprit lesions was safe and associated with favorable reduction of restenosis and repeat revascularization. © 2010 Wiley‐Liss, Inc.