bluuebunny/crossref_metadata_2025_split huggingface dataset:
Rapid Screening of Lectins for Multivalency Effects with a Glycodendrimer Microarray
Authors: Parera Pera Núria , Branderhorst Hilbert M. , Kooij Raymond , Maierhofer Caroline , van der Kaaden Marjolein , Liskamp Rob M. J. , Wittmann Valentin , Ruijtenbeek Rob , Pieters Roland J. 
Published: July 2010

AbstractMultivalency is an important phenomenon in protein–carbohydrate interactions. In order to evaluate glycodendrimers as multivalent inhibitors of carbohydrate binding proteins, we displayed them on a microarray surface. Valencies were varied from 1 to 8, and corrections were made for the valencies so that all surfaces contained the same amount of the sugar ligand. Five different carbohydrates were attached to the dendrimers. A series of fluorescent lectins was evaluated, and for each of them a binding profile was obtained from a single experiment showing both the specificity of the lectin for a certain sugar and whether it prefers multivalent ligands or not. Very distinct binding patterns were seen for the various lectins. The results were rationalized with respect to the interbinding distances of the lectins.
ChemInform Abstract: A Convenient Transformation of 2‐Alkylidenecycloalkanones into Alkyl‐Substituted Bicyclo[n.1.0]alkan‐1‐ols: Application to the Synthesis of Capsaicin.
Authors: Kananovich Dzmitry G. , Zubrytski Dzmitry M. , Kulinkovich Oleg G. 
Published: July 2010

AbstractAn efficient procedure is developed via conditions A) (formation of β‐iodoketones) followed by conditions B) in the presence of either TmsCl or TiCl(O‐iPr)3.
Conformational dynamics of actin: Effectors and implications for biological function
Authors: Hild Gábor , Bugyi Beáta , Nyitrai Miklós 
Published: July 2010

AbstractActin is a protein abundant in many cell types. Decades of investigations have provided evidence that it has many functions in living cells. The diverse morphology and dynamics of actin structures adapted to versatile cellular functions is established by a large repertoire of actin‐binding proteins. The proper interactions with these proteins assume effective molecular adaptations from actin, in which its conformational transitions play essential role. This review attempts to summarise our current knowledge regarding the coupling between the conformational states of actin and its biological function.
Deoxycitidine kinase is associated with prolonged survival after adjuvant gemcitabine for resected pancreatic adenocarcinoma
Authors: Maréchal Raphaël , Mackey John R. , Lai Raymond , Demetter Pieter , Peeters Marc , Polus Marc , Cass Carol E. , Salmon Isabelle , Devière Jacques , Van Laethem Jean‐Luc 
Published: July 2010

AbstractBACKGROUND:Gemcitabine (2′,2′‐difluorodeoxycytidine) administration after resection of pancreatic cancer improves both disease‐free survival (DFS) and overall survival (OS). Deoxycytidine kinase (dCK) mediates the rate‐limiting catabolic step in the activation of gemcitabine. The authors of this report studied patient outcomes according to the expression of dCK after a postoperative gemcitabine‐based chemoradiation regimen.METHODS:Forty‐five patients with resected pancreatic adenocarcinoma received adjuvant gemcitabine based‐therapy in the context of multicenter phase 2 studies. Their tumors were evaluated retrospectively for dCK protein expression by immunohistochemistry. A composite score based on the percentage of dCK‐positive cancer cells and the intensity of staining was generated, and the results were dichotomized at the median values.RESULTS:The median follow‐up was 19.95 months (95% confident interval [CI], 3.3‐107.4 months). The lymph node (LN) ratio and dCK protein expression were significant predictors of DFS and OS in univariate analysis. On multivariate analysis, dCK protein expression was the only independent prognostic variable (DFS: hazard ratio [HR], 3.48; 95% CI, 1.66‐7.31; P = .001; OS: HR, 3.2; 95% CI,1.44‐7.13; P = .004).CONCLUSIONS:dCK protein expression was identified as an independent and strong prognostic factor in patients with resected pancreatic adenocarcinoma who received adjuvant gemcitabine therapy. The authors concluded that it deserves prospective evaluation as a predictive biomarker for patient selection. Cancer 2010. © 2010 American Cancer Society.
Implicit processing goals combine with explicit goal standards to motivate performance through underlying comparison processes
Authors: Bittner Jenny V. 
Published: July 2010

AbstractThis research investigates whether explicit goals interact with implicit processing goals that are activated simultaneously. Based on the idea that (a) explicit goals are used as comparison standards, and (b) implicit processing goals have motivational consequences by influencing the process of comparison with those standards, these studies elucidate the mechanism by which explicit and implicit goals combine to influence task performance. Study 1 demonstrated that a primed goal to process similarities versus differences interacted with explicit goal standards in influencing subsequent task performance. High explicit goals resulted in better performance when participants had the implicit goal of processing similarities, whereas low explicit goals resulted in better performance when the implicit goal was processing differences. Study 2 provided evidence that perceived similarity to a target person is a critical factor for the pursuit of explicit goals and that this similarity influences task performance in the same way as the primed goal to process similarities. Study 3 indicated that processing similarities results from assimilation to a moderately high goal, whereas processing differences is the result of contrasting away from an extremely high goal. These findings confirm that implicit processing of similarities versus differences has a combined influence with explicit goals on task performance. Copyright © 2010 John Wiley & Sons, Ltd.
Effects of functionalized fullerenes on bifenthrin and tribufos toxicity to Daphnia magna: Survival, reproduction, and growth rate
Authors: Brausch Kathryn A. , Anderson Todd A. , Smith Philip N. , Maul Jonathan D. 
Published: July 2010

Abstract
Incorporation of carbon nanomaterials into industrial and consumer products is increasing, yet their impact on aquatic ecosystems alone and in chemical mixtures is largely unknown. Carbon nanomaterials may be found in the aquatic environment as mixtures with pesticides because of their proposed use in agriculture as smart delivery systems and nanosensors. The interaction effects of a functionalized fullerene ([1,2-methanofullerene C60]-61-carboxylic acid) (fC60) at 52.8 µg/L and the hydrophobic pesticides bifenthrin and tribufos were examined. The test organism was Daphnia magna, and response variables included 48-h survival, reproduction (bifenthrin, 70-d; tribufos, 21-d), and 10-d growth. Both pesticides reduced D. magna survival and reproduction (p &lt; 0.05). Fullerenes significantly increased bifenthrin acute toxicity but did not significantly affect chronic endpoints or growth (p &gt; 0.05). Median lethal concentrations (LC50s), median inhibition concentrations (IC50s) for days surviving, and IC50s for reproduction were 0.86, 0.55, and 0.49 µg/L for bifenthrin; 0.22, 0.39, and 0.77 µg/L for fC60-bifenthrin mix; 6.63, 9.89, and 5.79 µg/L for tribufos; and 9.17, 8.17, and 6.59 µg/L for fC60–tribufos mix. Mixtures did not affect instantaneous growth rate (p &gt; 0.05). These results suggest that fC60 had little effect on pesticide chronic toxicity but influenced acute toxicity. Given the widespread application of nanotechnology, the influence of nanomaterials on environmental contaminants is an important consideration. Thus, our results may be useful in the development and use of nanotechnology in agricultural practices. Environ. Toxicol. Chem. 2010;29:2600–2606. © 2010 SETAC
Tissue-based risk assessment of great blue heron (Ardea herodias) exposed to PCDD/DF in the Tittabawassee River floodplain, Michigan, USA
Authors: Seston Rita M. , Fredricks Timothy B. , Tazelaar Dustin L. , Coefield Sarah J. , Bradley Patrick W. , Newsted John L. , Kay Denise P. , Fitzgerald Scott D. , Giesy John P. , Zwiernik Matthew J. 
Published: July 2010

Abstract
Concentrations of dioxin-like compounds, primarily polychlorinated dibenzofurans (PCDFs), in soils and sediments of the Tittabawassee River (TR) and associated floodplains downstream of Midland, Michigan, USA, were greater than upstream sites and prompted a site-specific risk assessment of great blue herons (GBH). Tissue exposure of PCDF and polychlorinated dibenzo-p-dioxins (PCDDs) was assessed in multiple GBH tissue types, including blood plasma of adults and eggs, as well as blood plasma, adipose, liver, and muscle of nestlings. Adult GBH exposure was associated with foraging area and age class, with concentrations of PCDD/DF being greater in blood plasma of adult GBH foraging in the TR compared with those foraging in upstream reference areas and in older birds as compared with their younger cohorts. Concentrations of PCDD/DFs and dioxin-like polychlorinated biphenyls (PCBs) in eggs and nestling tissues of GBH collected from rookeries within the TR floodplain were generally similar among rookeries. Mean concentrations of PCDD/DFs in eggs of GBH ranged from 45 to 67 ng/kg, wet weight for the rookeries studied, with a maximum concentration of 210 ng/kg, wet weight observed. Adipose consistently had the greatest concentration of PCDD/DFs of all tissues collected from nestlings of GBH, ranging from 98 to 430 ng/kg, wet weight. Potential for adverse population-level effects from site-specific contaminant exposures were evaluated by comparison with selected toxicity reference values (TRVs). Minimal risk of adverse population-level effects were predicted when exposures measured in tissues of GBH collected from rookeries within the TR were compared with appropriate TRVs. This prediction is consistent with site-specific measures of population condition, which included clutch size and number of nestlings per successful nest. Environ. Toxicol. Chem. 2010;29:2544–2558. © 2010 SETAC
The effect of soil data resolution on hydrological processes modelling in a large humid watershed
Authors: Ye Xuchun , Zhang Qi , Viney N. R. 
Published: July 2010

AbstractHydrological process modelling depends on the data characterizing the heterogeneity of the catchment. In particular, in a large‐scale catchment, could a higher resolution of input data contribute to a more accurate result? In this study, surveyed soil data with two different spatial resolutions were used as input data for a Soil and Water Assessment Tool (SWAT) model simulation in a large humid watershed of Xinjiang River (15 535 km2) in Poyang Lake catchment in China. Simulations of stream flow, soil water storage and evapotranspiration (ET) generated using the two soil datasets were compared, and the impact of soil data resolution was analysed. The results indicate that the different resolutions of soil data have a great impact on the distribution of hydrological response units in the SWAT model, but do not lead to obvious differences in stream flow simulation. The finer resolution data produced a higher monthly soil water storage simulation than the lower‐resolution data across the watershed, while the seasonal variation of soil water content (SW) shows a close relation to the local precipitation and temperature. Results also show that the soil ET calculation method in the SWAT model is not particularly sensitive to the different resolutions of the soil maps. The implications of this study are that improvement of the resolution of soil data does not necessarily contribute to a more accurate prediction of stream flow in large‐scale watersheds. In practical studies, modellers need to select an appropriate resolution of soil data depending on the scale of watershed and the level of accuracy required, and also need to consider the principle of the model and the physical meaning of some key parameters to explain the simulation results. Copyright © 2010 John Wiley & Sons, Ltd.
Simultaneous color‐coded imaging to distinguish cancer “stem‐like” and non‐stem cells in the same tumor
Authors: Suetsugu Atsushi , Osawa Yosuke , Nagaki Masahito , Moriwaki Hisataka , Saji Shigetoyo , Bouvet Michael , Hoffman Robert M. 
Published: July 2010

AbstractIn this study, we demonstrate that the differential behavior, including malignancy and chemosensitivity, of cancer stem‐like and non‐stem cells can be simultaneously distinguished in the same tumor in real time by color‐coded imaging. CD133+ Huh‐7 human hepatocellular carcinoma (HCC) cells were considered as cancer stem‐like cells (CSCs), and CD133− Huh‐7 cells were considered as non‐stem cancer cells (NSCCs). CD133+ cells were isolated by magnetic bead sorting after Huh‐7 cells were genetically labeled with green fluorescent protein (GFP) or red fluorescent protein (RFP). In this scheme, CD133+ cells were labeled with GFP and CD133− cells were labeled with RFP. CSCs had higher proliferative potential compared to NSCCs in vitro. The same number of GFP CSCs and the RFP NSCCs were mixed and injected subcutaneously or in the spleen of nude mice. CSCs were highly tumorigenic and metastatic as well as highly resistant to chemotherapy in vivo compared to NSCCs. The ability to specifically distinguish stem‐like cancer cells in vivo in real time provides a visual target for prevention of metastasis and drug resistance. J. Cell. Biochem. 111: 1035–1041, 2010. © 2010 Wiley‐Liss, Inc.
High preoperative plasma fibrinogen levels are associated with distant metastases and impaired prognosis after curative resection in patients with colorectal cancer
Authors: Tang Liang , Liu Kai , Wang Junfeng , Wang Cui , Zhao Peng , Liu Jianzhong 
Published: July 2010

AbstractBackgroundThe purpose of this study was to evaluate the association between preoperative plasma fibrinogen levels on clinicopathologic parameters and overall survival in patients after curative resection with colorectal cancer.MethodsPreoperative plasma fibrinogen levels were examined in 341 patients who underwent curative resection for colorectal cancer. Preoperative plasma fibrinogen levels were correlated with clinicopathologic findings and disease‐specific overall survival.ResultsMean (±SD) preoperative plasma fibrinogen levels were 369.9 mg/dl (±69.1 mg/dl). Elevated plasma fibrinogen levels were associated with advanced tumor stage (P = 0.008), venous invasion (P = 0.006), and postoperative distant metastases (P < 0.001), but not with histologic grade (P = 0.232), invasion depth (P = 0.253), and lymph node involvement (P = 0.136). Multivariate analysis showed that preoperative plasma fibrinogen levels (P = 0.029), histologic grade (P = 0.001), and lymph node involvement (P = 0.001) were defined as independent prognostic factors.ConclusionsHigh preoperative plasma fibrinogen levels are associated with distant metastases and impaired prognosis after curative resection in patients with colorectal cancer. J. Surg. Oncol. 2010;102:428–432. © 2010 Wiley‐Liss, Inc.
A novel automatic method for monitoring Tourette motor tics through a wearable device
Authors: Bernabei Michel , Preatoni Ezio , Mendez Martin , Piccini Luca , Porta Mauro , Andreoni Giuseppe 
Published: July 2010

AbstractThe aim of this study was to propose a novel automatic method for quantifying motor‐tics caused by the Tourette Syndrome (TS). In this preliminary report, the feasibility of the monitoring process was tested over a series of standard clinical trials in a population of 12 subjects affected by TS. A wearable instrument with an embedded three‐axial accelerometer was used to detect and classify motor tics during standing and walking activities. An algorithm was devised to analyze acceleration data by: eliminating noise; detecting peaks connected to pathological events; and classifying intensity and frequency of motor tics into quantitative scores. These indexes were compared with the video‐based ones provided by expert clinicians, which were taken as the gold‐standard. Sensitivity, specificity, and accuracy of tic detection were estimated, and an agreement analysis was performed through the least square regression and the Bland‐Altman test. The tic recognition algorithm showed sensitivity = 80.8% ± 8.5% (mean ± SD), specificity = 75.8% ± 17.3%, and accuracy = 80.5% ± 12.2%. The agreement study showed that automatic detection tended to overestimate the number of tics occurred. Although, it appeared this may be a systematic error due to the different recognition principles of the wearable and video‐based systems. Furthermore, there was substantial concurrency with the gold‐standard in estimating the severity indexes. The proposed methodology gave promising performances in terms of automatic motor‐tics detection and classification in a standard clinical context. The system may provide physicians with a quantitative aid for TS assessment. Further developments will focus on the extension of its application to everyday long‐term monitoring out of clinical environments. © 2010 Movement Disorder Society
Adverse drug reactions to dopamine agonists: A comparative study in the french pharmacovigilance database
Authors: Perez‐Lloret Santiago , Bondon‐Guitton Emmanuelle , Rascol Olivier , Montastruc Jean‐Louis ,   and the French Association of Regional Pharmacovigilance Centers
Published: July 2010

AbstractPharmacodynamical differences between dopamine agonists (DAs) suggest differences in their adverse drug reactions (ADRs) profile. In this study, frequencies of ADR to DAs or levodopa reports in the French Pharmacovigilance Database were explored. Reports occurring between January 1, 1984 and December 31, 2008 were selected (2,189 for DAs and 1,315 for levodopa). The numbers of ADRs by system organ class were compared using ropinirole as a reference. Diurnal somnolence was less frequently reported with all DAs when compared with ropinirole (P < 0.001). Impulse control disorders (ICDs) were more frequently reported with pramipexole (P < 0.001). Significant difference was found among DAs in the frequency of confusion or disorientation (P < 0.001), nausea and vomiting (P < 0.05), or edemas (P < 0.001). No difference among DAs was observed in the frequency of hallucination or arterial hypotension ADR reports (P = 0.3 and P = 0.1). Pleural effusions were more frequently reported with pergolide or bromocriptine (P < 0.001). Somnolence or ICD reports were less frequent with levodopa, whereas confusion was more frequently reported. In summary, our data show significant differences in the kind of ADRs reported for each DA. © 2010 Movement Disorder Society.
Early‐onset L‐dopa‐responsive parkinsonism with pyramidal signs due to ATP13A2, PLA2G6, FBXO7 and spatacsin mutations
Authors: Paisán‐Ruiz Coro , Guevara Rocio , Federoff Monica , Hanagasi Hasmet , Sina Fardaz , Elahi Elahe , Schneider Susanne A. , Schwingenschuh Petra , Bajaj Nin , Emre Murat , Singleton Andrew B. , Hardy John , Bhatia Kailash P. , Brandner Sebastian , Lees Andrew J. , Houlden Henry 
Published: July 2010

AbstractSeven autosomal recessive genes associated with juvenile and young‐onset Levodopa‐responsive parkinsonism have been identified. Mutations in PRKN, DJ‐1, and PINK1 are associated with a rather pure parkinsonian phenotype, and have a more benign course with sustained treatment response and absence of dementia. On the other hand, Kufor‐Rakeb syndrome has additional signs, which distinguish it clearly from Parkinson's disease including supranuclear vertical gaze palsy, myoclonic jerks, pyramidal signs, and cognitive impairment. Neurodegeneration with brain iron accumulation type I (Hallervorden‐Spatz syndrome) due to mutations in PANK2 gene may share similar features with Kufor‐Rakeb syndrome. Mutations in three other genes, PLA2G6 (PARK14), FBXO7 (PARK15), and Spatacsin (SPG11) also produce clinical similar phenotypes in that they presented with rapidly progressive parkinsonism, initially responsive to Levodopa treatment but later, developed additional features including cognitive decline and loss of Levodopa responsiveness. Here, using homozygosity mapping and sequence analysis in families with complex parkinsonisms, we identified genetic defects in the ATP13A2 (1 family), PLA2G6 (1 family) FBXO7 (2 families), and SPG11 (1 family). The genetic heterogeneity was surprising given their initially common clinical features. On careful review, we found the FBXO7 cases to have a phenotype more similar to PRKN gene associated parkinsonism. The ATP13A2 and PLA2G6 cases were more seriously disabled with additional swallowing problems, dystonic features, severe in some, and usually pyramidal involvement including pyramidal weakness. These data suggest that these four genes account for many cases of Levodopa responsive parkinsonism with pyramidal signs cases formerly categorized clinically as pallido‐pyramidal syndrome. © 2010 Movement Disorder Society.
No increased risk of obstructive sleep apnea in Parkinson's disease
Authors: Trotti Lynn Marie , Bliwise Donald L. 
Published: July 2010

AbstractPulmonary function abnormalities in Parkinson's disease (PD) might predispose patients to obstructive sleep apnea (OSA) and daytime sleepiness. Fifty‐five idiopathic PD patients (mean age = 63.9) underwent three consecutive nights of in‐laboratory polysomnography on their usual dopaminergic medications. Sleep apnea severity was compared to published, normative, population‐based data from the Sleep Heart Health Study. Demographic and clinical data were compared in patients with and without OSA. The apnea‐hyponea index (AHI) was stable across nights in PD patients, and was not different between PD patients and normative controls. Epworth Sleepiness Scale scores, Body Mass Index, and snoring did not correlate with AHI. Severity of OSA is stable across multiple nights in PD patients. Rates of OSA in PD are similar to those seen in the general population. Daytime sleepiness, snoring, and obesity may not be helpful in identifying OSA in PD. © 2010 Movement Disorder Society
Restless legs syndrome in patients with amyotrophic lateral sclerosis
Authors: Lo Coco Daniele , Piccoli Federico , La Bella Vincenzo 
Published: July 2010

AbstractWe aimed to evaluate the frequency and determinants of restless legs syndrome (RLS) in a group of 76 patients with amyotrophic lateral sclerosis (ALS) and 100 control subjects. A diagnosis of RLS was made according to the criteria of the International RLS Study Group, and severity was assessed by the RLS severity scale. RLS was significantly more frequent in patients with ALS (ALS/RLS+) than in control subjects (25% vs. 8%; P = 0.002). Compared with control subjects, patients with ALS/RLS+ showed shorter history of RLS complaints and higher frequency of symptoms occurrence. Moreover, compared with those without RLS, patients with ALS/RLS+ showed increased functional impairment and more often reported sleep complaints. Multivariate logistic regression confirmed the association between RLS and functional impairment. Our findings suggest that RLS should be considered as a possible cause of disrupted sleep in patients with ALS and should be specifically investigated in these patients. © 2010 Movement Disorder Society.