bluuebunny/crossref_metadata_2025_split huggingface dataset:
Spatiotemporal distribution pattern of white matter lesion volumes and their association with regional grey matter volume reductions in relapsing‐remitting multiple sclerosis
Authors: Bendfeldt Kerstin , Blumhagen Jan Ole , Egger Hanspeter , Loetscher Patrick , Denier Niklaus , Kuster Pascal , Traud Stefan , Mueller‐Lenke Nicole , Naegelin Yvonne , Gass Achim , Hirsch Jochen , Kappos Ludwig , Nichols Thomas E , Radue Ernst‐Wilhelm , Borgwardt Stefan J. 
Published: January 2010

AbstractThe association of white matter (WM) lesions and grey matter (GM) atrophy is a feature in relapsing‐remitting multiple sclerosis (RRMS). The spatiotemporal distribution pattern of WM lesions, their relations to regional GM changes and the underlying dynamics are unclear. Here we combined parametric and non‐parametric voxel‐based morphometry (VBM) to clarify these issues. MRI data from RRMS patients with progressive (PLV, n = 45) and non‐progressive WM lesion volumes (NPLV, n = 44) followed up for 12 months were analysed. Cross‐sectionally, the spatial WM lesion distribution was compared using lesion probability maps (LPMs). Longitudinally, WM lesions and GM volumes were studied using FSL‐VBM and SPM5‐VBM, respectively. WM lesions clustered around the lateral ventricles and in the centrum semiovale with a more widespread pattern in the PLV than in the NPLV group. The maximum local probabilities were similar in both groups and higher for T2 lesions (PLV: 27%, NPLV: 25%) than for T1 lesions (PLV: 15%, NPLV 14%). Significant WM lesion changes accompanied by cortical GM volume reductions occured in the corpus callosum and optic radiations (P = 0.01 corrected), and more liberally tested (uncorrected P < 0.01) in the inferior fronto‐occipital and longitudinal fasciculi, and corona radiata in the PLV group. Not any WM or GM changes were found in the NPLV group. In the PLV group, WM lesion distribution and development in fibres, was associated with regional GM volume loss. The different spatiotemporal distribution patterns of patients with progressive compared to patients with non‐progressive WM lesions suggest differences in the dynamics of pathogenesis. Hum Brain Mapp, 2010. © 2010 Wiley‐Liss, Inc.
Resveratrol, a multitargeted agent, can enhance antitumor activity of gemcitabine in vitro and in orthotopic mouse model of human pancreatic cancer
Authors: Harikumar Kuzhuvelil B. , Kunnumakkara Ajaikumar B. , Sethi Gautam , Diagaradjane Parmeswaran , Anand Preetha , Pandey Manoj K. , Gelovani Juri , Krishnan Sunil , Guha Sushovan , Aggarwal Bharat B. 
Published: January 2010

AbstractGemcitabine, while a standard treatment of advanced pancreatic cancer (PaCa), alone is not very effective. New agents that are safe and effective are highly needed. Resveratrol is one such agent which is safe and multitargeted; and has been linked with suppression of survival, proliferation, invasion and angiogenesis of cancer. Whether resveratrol can sensitize PaCa to gemcitabine in vitro and in vivo was investigated. We established PaCa xenografts in nude mice, randomized into 4 groups, and treated with vehicle, gemcitabine, resveratrol and with combination. Modulation of NF‐κB and markers of proliferation, angiogenesis and invasion were ascertained using electrophoretic mobility shift assay (EMSA), immunohistochemistry and western blot analysis. Resveratrol inhibited the proliferation of 4 different human PaCa cell lines, synergized the apoptotic effects of gemcitabine, inhibited the constitutive activation of NF‐κB and expression of bcl‐2, bcl‐xL, COX‐2, cyclin D1 MMP‐9 and VEGF. In an orthotopic model of human PaCa, we found that resveratrol significantly suppressed the growth of the tumor (p < 0.001) and this effect was further enhanced by gemcitabine (p < 0.001). Both the markers of proliferation index Ki‐67 and the micro vessel density CD31 were significantly downregulated in tumor tissue by the combination of gemcitabine and resveratrol (p < 0.001 vs. control; p < 0.01 vs. gemcitabine). As compared to vehicle control, resveratrol also suppressed the NF‐κB activation and expression of cyclin D1, COX‐2, ICAM‐1, MMP‐9 and survivin. Overall our results demonstrate that resveratrol can potentiate the effects of gemcitabine through suppression of markers of proliferation, invasion, angiogenesis and metastasis.
IL‐1α and IL‐1β have different effects on formation and activity of large osteoclasts
Authors: Trebec‐Reynolds Diana P. , Voronov Irina , Heersche Johan N.M. , Manolson Morris F. 
Published: January 2010

AbstractInterleukin 1 (IL‐1) is a proinflammatory cytokine upregulated in conditions such as rheumatoid arthritis and periodontal disease. Both isoforms, IL‐1α and IL‐1β, have been shown to activate osteoclasts (OCs), the cells responsible for resorbing bone. Inflammatory conditions are also characterized by increased bone loss and by the presence of large OCs (10+ nuclei). We and others have previously shown that large OCs are more likely to be resorbing compared to small OCs (2–5 nuclei). Moreover, large OCs express higher levels of the IL‐1 activating receptor IL‐1RI, integrins αv and β3, RANK, and TNFR1, while small OCs have higher levels of the decoy receptor IL‐1RII. We hypothesized that IL‐1 would have different effects on large and small OCs due to these distinct receptor expression patterns. To test this hypothesis, RAW 264.7 cells were differentiated into populations of small and large OCs and treated with IL‐1α or IL‐1β (1 and 10 ng/ml). In the presence of sRANKL, both IL‐1α and IL‐1β increased total OC number and resorptive activity of large OCs. IL‐1α stimulated formation of large OCs and increased the number of resorption pits, while IL‐1β changed the morphology of large OCs and integrin‐β3 phosphorylation. No effects were seen in small OCs in response to either IL‐1 isoform. These results demonstrate that IL‐1 predominantly affects large OCs. The dissimilarity of responses to IL‐1α and IL‐1β suggests that these isoforms activate different signaling pathways within the two OC populations. J. Cell. Biochem. 109: 975–982, 2010. © 2010 Wiley‐Liss, Inc.
Physcion‐8‐O‐β‐D‐glucopyranoside enhances the commitment of mouse mesenchymal progenitors into osteoblasts and their differentiation: Possible involvement of signaling pathways to activate BMP gene expression
Authors: Lee Su‐Ui , Choi Yeon Hee , Kim Young Sup , Park Sang‐Joon , Kwak Han Bok , Min Yong Ki , Kim Hyun‐Nam , Lim Kyung‐Eun , Choi Je‐Yong , Rhee Myungchull , Kim Seong Hwan 
Published: January 2010

AbstractHere, we show the involvement of signaling pathways to induce the gene expression of bone morphogenetic protein (BMP) in the osteogenic activity of physcion‐8‐O‐β‐D‐glucopyranoside (physcion‐Glu); it stimulated osteoblast differentiation in mouse osteoblast MC3T3‐E1 subclone 4 cells and induced BMP‐2 gene expression and activation of Akt and ERK/MAP kinases. Physcion‐Glu‐induced BMP‐2 expression and mineralization were attenuated by LY294002, an inhibitor of PI3K that lies upstream of Akt and MAP kinases, suggesting that physcion‐Glu induces osteoblast differentiation via PI3K‐Akt/MAP kinase signaling pathways, which play important roles in inducing BMP‐2 gene expression. Physcion‐Glu also enhanced BMP‐2‐induced commitment of mouse bi‐potential mesenchymal precursor C2C12 cells into osteoblasts while inducing the transcription of several osteogenic BMP isoforms, such as BMP‐2, ‐4, ‐7, and ‐9. Osteogenic synergy between BMP‐2 and physcion‐Glu was supported by the fact that noggin inhibited BMP‐2 and physcion‐Glu‐induced alkaline phosphatase expression and activity. Considering that physcion‐Glu induced Runx2 activity and the nuclear translocation of p‐Smad, physcion‐Glu could act by enhancing the BMP signaling pathway that induces Smad activation and translocation to activate Runx2. In conclusion, physcion‐Glu could enhance the commitment of mesenchymal progenitors into osteoblasts and their differentiation by activating signaling pathways to induce BMP gene expression. J. Cell. Biochem. 109: 1148–1157, 2010. © 2010 Wiley‐Liss, Inc.
Development and initial psychometric properties of the University of Texas at San Antonio Future Disposition Inventory
Authors: Osman Augustine , Gutierrez Peter M. , Barrios Frank , Wong Jane L. , Freedenthal Stacey , Lozano Gregorio 
Published: January 2010

AbstractWe describe the development and initial psychometric properties of a 24‐item self‐report measure, the University of Texas at San Antonio Future Disposition Inventory (UTSA FDI). This instrument is designed to evaluate future related thoughts and feelings that are associated with suicide‐related risk behaviors: positive focus, suicide orientation, and negative focus. The items were generated by clinical and nonclinical samples. The structure of the instrument was defined in a sample of 350 university undergraduate students (Study 1). Using CFA, we confirmed the fit of the 3‐factor solution in an independent sample of college age students (n=452; Study 2). Scale reliability estimates were good (all ρ's≥.80) in both studies. Known‐groups validation analyses showed that scores on each scale were useful in differentiating the responses of the study groups. Correlates for the scales were identified when scores on measures of suicide‐related risk and general psychological symptoms were included as validation self‐report instruments. © 2010 Wiley Periodicals, Inc. J Clin Psychol 66: 1–20, 2010.
Distribution of free D‐aspartic acid and D‐aspartate oxidase in frog Rana esculenta tissues
Authors: Di Giovanni Marcello , Burrone Lavinia , Chieffi Baccari Gabriella , Topo Enza , Santillo Alessandra 
Published: January 2010

AbstractIn this paper, we examined the distribution pattern of D‐aspartic acid (D‐Asp), as well as D‐aspartate oxidase (D‐AspO), D‐amino acid oxidase (D‐AAO), and L‐amino acid oxidase (L‐AAO) activities in different tissues of frog, Rana esculenta. High concentrations of free D‐Asp were found in the testes (0.21±0.02 µmol/g b.w), in the liver (0.20±0.03 µmol/g b.w), and in the Harderian gland (HG) (0.19±0.03 µmol/g b.w). A higher activity of both D‐AspO and D‐AAO with respect to L‐AAO was endogenously present in all examined frog tissues, particularly within the kidney, liver, and brain. Our in vivo experiments, consisting of i.p. injections of 2.0 µmol/g b.w. D‐Asp in frogs, revealed that all examined tissues can take up and accumulate D‐Asp and that this amino acid specifically triggers D‐AspO activity. Indeed, no increase in both D‐AAO and L‐AAO was found in all frog tissues after D‐Asp treatment. The optimum pH for D‐AspO activity was around 8.2 and the optimum temperature was about 37°C. Furthermore, its activity linearly increased with increasing D‐Asp incubation times. In vitro experiments assaying the substrate specificity of D‐AspO indicated that the enzyme had greater affinity for N‐methyl‐D‐aspartate than for D‐Asp and D‐glutamate. This study provides evidence of the presence of free D‐Asp in frog R. esculenta tissues, along with its role in triggering D‐AspO activity. These findings suggest that D‐AspO could play an essential role in decreasing excessive amounts of D‐Asp in frog tissues, a phenomenon that, if left unchecked, could have detrimental physiological effects on the animal. J. Exp. Zool. 313A:137–143, 2010. © 2010 Wiley‐Liss, Inc.
Altered product specificity of a cyclodextrin glycosyltransferase by molecular imprinting with cyclomaltododecaose
Authors: Kaulpiboon Jarunee , Pongsawasdi Piamsook , Zimmermann Wolfgang 
Published: January 2010

AbstractCyclodextrin glycosyltransferases (CGTases), members of glycoside hydrolase family 13, catalyze the conversion of amylose to cyclodextrins (CDs), circular α‐(1,4)‐linked glucopyranose oligosaccharides of different ring sizes. The CD containing 12 α‐D‐glucopyranose residues was preferentially synthesized by molecular imprinting of CGTase from Paenibacillus sp. A11 with cyclomaltododecaose (CD12) as the template molecule. The imprinted CGTase was stabilized by cross‐linking of the derivatized protein. A high proportion of CD12 and larger CDs was obtained with the imprinted enzyme in an aqueous medium. The molecular imprinted CGTase showed an increased catalytic efficiency of the CD12‐forming cyclization reaction, while decreased kcat/Km values of the reverse ring‐opening reaction were observed. The maximum yield of CD12 was obtained when the imprinted CGTase was reacted with amylose at 40°C for 30 min. Molecular imprinting proved to be an effective means toward increase in the yield of large‐ring CDs of a specific size in the biocatalytic production of these interesting novel host compounds for molecular encapsulations. Copyright © 2010 John Wiley & Sons, Ltd.
Rotenone prevents impact‐induced chondrocyte death
Authors: Goodwin Wendy , McCabe Daniel , Sauter Ellen , Reese Eric , Walter Morgan , Buckwalter Joseph A. , Martin James A. 
Published: January 2010

AbstractMechanical insult to articular cartilage kills chondrocytes, an event that may increase the risk of posttraumatic osteoarthritis. Recent reports indicate that antioxidants decrease impact‐induced chondrocyte death, but the source(s) of oxidants, the time course of oxidant release, and the identity of the oxidative species generated in response to injury are unknown. A better understanding of these processes could lead to new treatments of acute joint injuries. To that end, we studied the kinetics and distribution of oxidant production in osteochondral explants subjected to a single, blunt‐impact injury. We followed superoxide production by measuring the time‐dependent accumulation of chondrocyte nuclei stained with the superoxide‐sensitive probe dihydroethidium. The percentage of chondrocytes that were dihydroethidium‐positive was 35% above baseline 10 min after impact, and 65% above baseline 60 min after impact. Most positive cells were found within and near areas contacted directly by the impact platen. Rotenone, an electron transport chain inhibitor, was used to test the hypothesis that mitochondria contribute to superoxide release. Rotenone treatment significantly reduced dihydroethidium staining, which remained steady at 15% above baseline for up to 60 min postimpact. Moreover, rotenone reduced chondrocyte death in impact sites by more than 40%, even when administered 2 h after injury (p < 0.001). These data show that much of the acute chondrocyte mortality caused by in vitro impact injuries results from superoxide release from mitochondria, and suggest that brief exposure to free radical scavengers could significantly improve chondrocyte viability following joint injury. © 2010 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 28:1057–1063, 2010
Surface‐enhanced resonance Raman spectroscopic characterization of cytochrome c immobilized on 2‐mercaptoethanesulfonate monolayers on silver
Authors: Królikowska Agata , Bukowska Jolanta 
Published: January 2010

AbstractSingle‐component self‐assembled monolayers (SAMs) of mercaptoethanesulfonate (MES) on Ag surfaces were studied with surface‐enhanced resonance Raman scattering (SERRS) spectroscopy with a view to their application to immobilize (ferro)cytochrome c (cyt c). SERS studies revealed that MES molecules adopt primarily trans (T) conformation even at early stages of the SAM growth and over wide range of pH values. High accessibility of the negatively charged groups for (bio)molecules in solution makes single‐component MES SAMs suitable linkage monolayers for electrostatic attachment of cyt c, which was verified with SERRS. Cyt c was successfully anchored to MES‐coated Ag at natural (∼5), neutral, and isoelectric point (10.6) pH. At pH = 7.0 and 10.6, SERRS bands characteristic of native six‐coordinated low‐spin (6cLS) heme iron configuration were detected. Lack of buffering resulted in additional appearance of five‐coordinated high‐spin (5cHS) SERRS markers and the presence of bands indicating ferric ion. An electrostatic attraction between protein and SAM was confirmed to exclude the hydrophobic interactions involved in cyt c binding. Cyt c attached to MES SAM on Ag was found to be electroactive at neutral pH, and protein oxidation was assisted with reversible conversion of 6cLS to the non‐native 5cHS state. Alteration of heme orientation deduced from SERRS spectra upon change of redox state allowed us to propose the protein dynamics as the electron transfer rate‐limiting step. Copyright © 2010 John Wiley & Sons, Ltd.
The use of knowledge transfer mechanisms in franchising
Authors: Gorovaia Nina , Windsperger Josef 
Published: January 2010

AbstractIn this paper, we try to explain the use of knowledge transfer mechanisms in franchising firms by applying the knowledge‐based view of the firm that integrates results from the information richness theory. Starting from the information richness theory we argue that the degree of tacitness of system‐specific knowledge determines the information richness of the knowledge transfer mechanisms of franchising firms. We examine the following hypotheses: (1) the franchisor uses more knowledge transfer mechanisms with a lower degree of information richness (such as email, intra‐ and internet), if the tacitness of system‐specific knowledge is low, and (2) the franchisor uses more knowledge transfer mechanisms with a higher degree of information richness (such as training, seminar, meetings, visits), if the tacitness of system‐specific knowledge is high. We test these hypotheses by using data from 83 franchising firms in the Austrian franchise sector. The data provide partial support for the hypotheses. Copyright © 2010 John Wiley & Sons, Ltd.
Extending the task–artifact framework with organizational learning
Authors: Jiang Hao , Carroll John M , Lee Roderick 
Published: January 2010

AbstractOver the past decade there has been an increased focus on the importance of contextual factors in all IT disciplines. At the same time, scholars have heeded the call for investigating the creation of more effective synergies between science and design. For example, the task–artifact (TA) framework was developed to support better utilization of behavioral, cognitive, and social science in Human–Computer Interaction (HCI) design. This framework is based on the general developmental pattern of human activities and technologiesteamed with two analytical tools for design. In this paper, we extend this framework from its original focus on individual and small group HCI to the organizational level utilizing organizational learning theory. This extension will (1) integrate relevant concepts and analysis drawn from the social sciences in order to guide design more effectively; (2) incorporate organizational level analysis, when we study information artifact design and appropriation; and (3) help organizations complete their learning circle. Copyright © 2010 John Wiley & Sons, Ltd.
Optimal estimates for the Hardy averaging operator
Authors: Nekvinda Aleš , Pick Luboš 
Published: January 2010

AbstractLetbe the one‐dimensional Hardy averaging operator. It is well‐known thatAis bounded onLpwhenever 1 <p≤ ∞. We improve this result in the following sense: we introduce a pair of new function spaces, the ‘source’ spaceSp, which is strictly larger thanLp, and the ‘target’ spaceTp, which is strictly smaller thanLp, and prove thatAis bounded fromSpintoTp. Moreover, we show that this result cannot be improved within the environment of solid Banach spaces. We present applications of this result to variable‐exponent Lebesgue spacesLp(x)(© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Camptocormia in idiopathic Parkinson's disease: A focal myopathy of the paravertebral muscles
Authors: Margraf Nils G. , Wrede Arne , Rohr Axel , Schulz‐Schaeffer Walter J. , Raethjen Jan , Eymess Andrea , Volkmann Jens , Mehdorn Maximilian H. , Jansen Olav , Deuschl Günther 
Published: January 2010

AbstractThe objective of our study was to describe the clinical features of camptocormia, an involuntary, marked flexion of the thoracolumbar spine in idiopathic Parkinson's disease (PD) and to understand its etiology. In a prospective, cross‐sectional study, we examined 15 patients with PD and camptocormia using laboratory parameters, EMG, muscle magnetic resonance imaging, and biopsy of the paravertebral muscles. The clinical data were compared with a matched control group of PD patients without camptocormia, and the biopsies were compared with muscles from age‐matched autopsies. Almost all the patients (median age, 68.0 years; 7 women) with camptocormia suffered from advanced PD. Camptocormia occurred at a median of 9.0 years after the PD diagnosis. Compared with our clinical control group, back pain was more frequent and less dopa‐sensitive in the patients with camptocormia who suffered more often from additional diseases of the back. On EMG, we found mainly a myopathic pattern. The MRI of the paravertebral muscles showed localized changes ranging from edema with contrast enhancement, which are considered to be early signs, to atrophy and/or fatty degeneration, interpreted as late degenerative changes. Early signs were seen mainly during the first year and degenerative changes after 1.5 years. Biopsies revealed consistently myopathic changes and in some cases fatty degeneration. Clinical or electromyographic features favoring dystonia were absent. Camptocormia is a major disabling, non‐fluctuating and levodopa‐resistant complication of advanced PD. The cause of camptocormia in idiopathic PD is a focal myopathy. Our findings suggest that the myopathy has a progressive course, which finally leads to degeneration of the paravertebral muscles. © 2010 Movement Disorder Society
Incidence of Parkinson's disease and atypical parkinsonism: Russian population‐based study
Authors: Winter Yaroslav , Bezdolnyy Yury , Katunina Elena , Avakjan Gagik , Reese Jens P. , Klotsche Jens , Oertel Wolfgang H. , Dodel Richard , Gusev Eugene 
Published: January 2010

AbstractData on the incidence of Parkinson's disease (PD) and atypical parkinsonian syndromes (APS) in East European countries and Asia are limited. The objective of this prospective population‐based study was to determine the incidence of PD and APS in the Russian population. The study area was a large district of Moscow with a population of 1,237,900 inhabitants. Multiple sources of case ascertainment were used to identify incident cases of PD and APS between July 2006 and December 2008. All incident cases were examined by a specialist and followed up prospectively to confirm the diagnosis. The age‐standardized incidence rates per 100,000/year were 9.03 [95% confidence interval (CI) 8.01–10.15] for PD, 0.11 (95% CI 0.03–0.23) for multiple system atrophy, 0.14 (95% CI 0.08–0.21) for progressive supranuclear palsy, and 0.02 (95% CI 0.01–0.12) for corticobasal degeneration. The age‐standardized male‐to‐female ratio of PD was 0.87 for all ages and 1.46 for those aged 60 and older. A high proportion of new cases with PD (34%) and APS (50%) had comorbid depressive symptoms. Given the rapid growth of the elderly population in Eastern Europe and Asia, the epidemiology of PD and APS in these regions should be investigated in greater depth. The incidence of PD in our study was slightly lower than in studies of Western populations and the male‐to‐female ratio was closer to those reported in studies from Asia. The clinical implication of our study is that it highlights the need for better diagnosis and treatment of depression in early stages of PD. © 2010 Movement Disorder Society