bluuebunny/crossref_metadata_2025_split huggingface dataset:
Transfer of immature oocytes to a preovulatory follicle: An alternative to in vitro maturation in the mare?
Authors: GOUDET GHYLÈNE , BÉZARD JACQUELINE , DUCHAMP G. , PALMER E. 
Published: June 2010

SummaryIn the mare, success rates for the in vitro maturation of oocytes are low. Accordingly, we attempted to determine if immature oocytes could be matured in vivo by injecting them into a preovulatory follicle. Groups of 3–9 oocytes collected from donor mares were transferred under ultrasound control into the preovulatory follicle of a recipient mare that was treated with crude equine pituitary gonadotrophin (CEG) to induce ovulation. Just before ovulation (34 h post treatment) the preovulatory follicle of the recipient mare was punctured to collect both the transferred and the indigenous oocytes to analyse the stages of nuclear maturation.The transfer technique did not impair significantly the final maturation of the recipient preovulatory follicle. The indigenous oocytes within the recipient follicles were recognisable by their larger expanded cumulus of yellow colouration due to high hyaluronic acid content; 7/12 of these oocytes were mature (metaphase II). Around half (42/86; 49%) of the oocytes transferred to preovulatory follicles were recovered subsequently. Most of them showed cumulus expansion (41/42, 6 of which were rich in hyaluronic acid), 13 (32%) were mature, 15 (36%) were immature and 13 (32%) were degenerate. When the indigenous oocyte of the recipient mare was mature, 38% of the transferred oocytes were mature, this rate being no different from the in vitro maturation rate of 46%.This study showed that in vivo maturation of immature oocytes by transfer into a preovulatory follicle in a recipient mare is possible. The maturation rate is not different from the in vitro maturation rate. The technique allows the generation of mature oocytes that have an expanded cumulus rich in hyaluronic acid, similar to the situation in preovulatory oocytes. This result has not been obtained in vitro previously.
A permeability barrier in the dorsal wall of the equine hoof capsule
Authors: KEMPSON SUSAN A. , CAMPBELL ELAINE H. 
Published: June 2010

SummaryThe permeability barrier in the dorsal wall of the equine hoof capsule was studied by means of horseradish peroxidase (HRP) in 0.9 N saline solution as a water soluble tracer. Sections were treated with 3′3′‐diaminobenzidine tetrachloride (DAB) and before dissection the quality of the horn of feet from 10 horses was assessed and given a subjective grade as either good or poor. Blocks of tissue from each horse were left in either an oven at 60°C or in water for 2 weeks before treatment in HRP, sectioning and DAB solution. Regions observed were i) outer surface, ii) outermost layers of the horn, iii) cut edge of the outer layer, iv) inner layer of horn, v) cut edge of the inner layer and vi) laminae. Horn deemed to be normal horn and of good ‘quality’ showed very slight penetration of HRP 3–5 cell layers deep in the outer layer. The cut edge of the outer layer of the wall of the ‘normal’ horn also showed minimal penetration of HRP through the intercellular spaces. The cut edge of the inner layers of the wall of normal, good quality horn showed penetration of the tracer up to 20 cell layers deep, with HRP in both the intercellular spaces and within the cells. In contrast, sections of horn from horses with brittle feet showed deep cracks in the outer surface into which the HRP had penetrated.Good quality horn showed no change in the position of the permeability barrier after soaking in water for 14 days, but the brittle horn showed an increase in permeability to HRP. In brittle horn, reaction product was seen deep within the section in the intercellular spaces of the intertubular horn only. Placing horn in an oven had no effect on the permeability barrier. The permeability barrier of the dorsal wall of the equine hoof capsule differs with the layer of the wall. Horn considered to be of poor quality had a weaker permeability barrier than horn of good quality.
Onychomycosis in white line disease in horses: pathology, mycology and clinical features
Authors: KUWANO A. , YOSHIHARA T. , TAKATORI K. , KOSUGE J. 
Published: June 2010

SummaryThis paper describes onychomycosis in horses and reports the pathological findings, associated fungi and incidence of concurrent white line disease. In addition to these observations, relevance between post mortem and clinical findings of onychomycosis are discussed in 3 necropsied horses. Samples were collected from 100 hooves from a total of 51 Thoroughbreds suffering from white line disease. Of these, 15 hooves from 13 horses were also complicated with severe hoof wall fissure formation. Preparations from the same samples were used both for histopathology and for culture to identify the associated fungi. Onychomycosis was diagnosed when it could be confirmed histologically. Fungal identification was based on morphological characteristics in culture and the associated fungi were determined by comparison with culture morphology. As a result, 10 samples collected from horses were diagnosed as suffering from onychomycosis. Seven of these showed complicating severe inner hoof wall fissures and the soil fungus Scedosporium apiospermum or the teleomorph of this fungus, i.e., Pseudallescheria boydii, was isolated. The fungus was found to be ubiquitous in the fissure cavities, the terminal horn of the white line and the terminal horn‐like laminae of the metaplastic white line‐like tissue. It can be concluded that onychomycosis frequently causes white line disease and/or makes it worse. Associated with deterioration of the submural condition, the main associated fungus for onychomycosis in this series, was Genus Scedosporium and the most susceptible region was the terminal horn of the hypertrophied white line and/or the terminal horn‐like laminae of the metaplastic white line‐like tissue.
Effect of supplementary dietary biotin on hoof growth and hoof growth rate in ponies: a controlled trial
Authors: REILLY J. D. , COTTRELL D. F. , MARTIN R. J. , CUDDEFORD D. J. 
Published: June 2010

SummaryThe effect of dietary biotin supplementation, at a dose rate of 0.12 mg/kg bwt, on growth and growth rate of the hooves of 8 match‐paired ponies was investigated in a controlled feeding trial. Treatment animals had a mean hoof growth at the midline dead centre of the hoof capsule of 35.34 mm after 5 months of biotin supplementation compared to control animals 30.69 nun (P<0.05). Comparison of regression analysis also showed that biotin supplementation produced a significantly higher (P<0.02) growth rate of hoof horn in this trial. Treatment animals had a 15% higher growth rate of hoof horn and 15% more hoof growth at the midline dead centre, after 5 months of biotin supplementation compared to control ponies. No differences were found between feet for growth of horn, but the older animals in the trial had significantly lower hoof growth (P<0.05) than the remaining ponies.
Equine laminitis: its development coincides with increased sublamellar blood flow
Authors: POLLITT C. C. , DAVIES C. T. 
Published: June 2010

SummaryThe effect of alimentary carbohydrate overload on hoof temperature was investigated to determine the state of the sublamellar vasculature preceding the onset of equine laminitis. Hoof, core and ambient temperatures and heart rate were logged continuously in 21 mature Standardbred horses kept in an environmental chamber set at 10°C. Recording hoof temperature was a successful, noninvasive, method to measure indirectly, shifts in digital blood flow against a background of cold induced, physiological, vasoconstriction. High hoof temperatures were assumed to indicate digital vasodilation and low hoof temperatures digital vasoconstriction. Seven horses were either untreated or sham treated controls. A slurry of ground wheat flour (17.5 g/kg) was administered via nasogastric tube to 13 horses all of which were humanely killed 48 h later. Histological sections of the lamellar tissues were examined for evidence of laminitis. Analysis of mean hoof temperature graphs showed that horses judged laminitis positive had experienced a period of prolonged digital vasodilation 16–40 h after carbohydrate overload. Laminitis negative horses experienced no such period of vasodilation and never had hoof temperatures significantly (except once, at 28 h) above that of controls. The only parameter which significantly differentiated the laminitis positive from laminitis negative horses, between 12 and 32 h after carbohydrate overload, was foot temperature, which was significantly higher in laminitis positive horses (P<0.05). Therefore, a period of sublamellar vasodilation, 12 to 40 h after alimentary carbohydrate overload precedes the onset of laminitis. If the digital circulation sustains vasoconstriction during this period then laminitis does not occur. We propose that the period of increased digital blood flow in laminitis positive horses, concomitant with the severe metabolic crisis brought on by the alimentary carbohydrate overload, may expose the lamellar tissues to a concentration of blood borne factors sufficient to trigger lamellar separation.
Characterisation of headshaking syndrome −31 cases
Authors: MADIGAN J. E. , BELL STEPHANIE A. 
Published: June 2010

SummaryHeadshaking is a maturity onset condition with the most commonly reported clinical signs being 'flipping' of the nose, nose rubbing, snorting or sneezing, and acting like a bee is flying up the nostril. A questionnaire was completed by owners of 31 horses with headshaking syndrome. The history, time of onset, clinical presentation and treatment of this condition were reported. Headshaking appeared to be light‐stimulated in approximately 60% of the horses. The condition is seasonal and recurring in the majority of horses. Treatment with cyproheptadine produced improvement of symptoms in 76% of cases. The clinical signs are suggested to be compatible with neuropathic pain producing itching, tingling or electric like sensations in the face and muzzle area of affected horses.
Perceptions and attitudes towards horses in European societies
Authors: ENDENBURG N. 
Published: June 2010

SummaryIn spite of the fact that horses were domesticated between 5000 and 3000 B.C., the relations between humans and horses constitutes a relatively unexplored area. This may be due to the fact that horses in middle class western society are seen as companion animals, of primarily social and recreational value. Horses, however, have historically enjoyed an enormously important utilitarian, economic and sporting significance and in many countries horse meat is also eaten.Nowadays, we can classify the different ways in which horses are used into four different fields:• recreational and/or social purposes• breeding• sport and competition• meat productionIn each category one can distinguish 4 main areas of concern:• the behaviour and wellbeing of the horse• feeding• maintenance• veterinary careAll 4 fields make their own particular demands on these aspects of the care of horses. In addition, people have also approached these different fields of equine exploitation through the centuries with different attitudes. The goal of this article is to review the attitudes of people towards horses. The main conclusion of this article is that the diversity of uses for equines and corresponding organisations makes it difficult to get a clear overview and thereby inhibits scientific research into human‐horse interactions.
Comparative pathophysiology of nonglandular ulcer disease: a review of experimental studies
Authors: ARGENZIO R. A. 
Published: June 2010

SummaryUlceration of the nonglandular, stratified squamous mucosa of the equine and porcine stomach is a common event in both species, although in pigs the fatality rate is significant and it is an economically important disease. Because the barrier function of this mucosa in horses and pigs appears similar, it is probable that similar pathophysiological mechanisms may be responsible for the initiating lesions and reparative events. Recent studies of ulcer pathogenesis in the pig have shown that feed preparation or prolonged fasting can result in disruption of the normal stratification of gastric contents, thereby allowing high concentrations of HCl, pepsin and refluxed bile to mix in the proximal stomach. Conditions simulating those foundin vivohave been shown to damage this mucosain vitroand indicate that luminal products, such as short chain fatty acids and bile salts, which act in synergy with HCl, probably are necessary to induce significant damage to this mucosa. Studies of the equine stomach have shown a similar proximal to distal pH gradient in the fed stomach, a significant duodenal‐gastric reflux, and induction of squamous ulcers with fasting, thereby illustrating that similar conditions may be responsible for damage to the equine nonglandular mucosa.
Induction and maintenance of gastric ulceration in horses in simulated race training
Authors: VATISTAS N. J. , SIFFERMAN R. L. , HOLSTE J. , COX J. L. , PINALTO G. , SCHULTZ K. T. 
Published: June 2010

SummaryGastric ulceration is a prevalent condition of racehorses. A number of models of gastric ulceration have been described, but none mimic the conditions of a horse in training. The objectives of this study were to determine whether gastric ulcers could be induced and maintained in a group of horses in simulated race training. In addition, serum Cortisol was measured on a weekly basis to investigate the possibility that stress may be important in the pathogenesis of gastric ulceration. Thirty horses used in the trial were fed Bermuda grass hay and 6 kg of a concentrate diet, and exercised 6 days/week at speed over a distance of 1.6–2.4 km. Serum was collected and gastroendoscopic examinations performed on a weekly basis for the duration of the trial. All horses developed moderate to severe ulceration, and ulcers were maintained for the 56 day period of the trial. Only one horse had signs of abdominal discomfort, which resolved with minimal symptomatic treatment and without the use of antiulcer medications. Serum Cortisol remained within reference ranges for the duration of the trial. Although there was some variation between the weekly examinations, serum Cortisol concentrations were decreased from values obtained at the start of the trial. In this study ulcers developed without the administration of nonsteroidal anti‐inflammatory agents or withholding of feed. This model provides a method to study the condition, and to investigate the effects of medications on the healing of ulcers in racehorses.
Investigation of an outbreak of tapeworm‐associated colic in a training yard
Authors: PROUDMAN C. J. , HOLDSTOCK N. B. 
Published: June 2010

SummaryA novel serological assay which measures IgG(T) specific for a 12/13 kDa protein of the equine tapewormAnoplocephala perfoliatawas used as part of a colic outbreak investigation. A training/rehabilitation yard for Thoroughbreds and Arabs was found to have an increasing incidence of colic over a 5 year period, culminating in a peak incidence of 1.15 episodes/horse year at risk. Four animals suffered from ileal impaction colic which necessitated surgical management. A case‐control study design suggested a strong association between tapeworm infection and colic, with evidence of a dose‐response relationship. Intervention, in the form of anticestode anthelminthics, coincided with a decrease in the incidence of colic and a fall in anti‐12/13 kDa IgG(T) antibody levels of 8 horses monitored post‐treatment. This study demonstrates that anthelminthic regimens, using exclusively ivermectin, may lead to an increase in tapeworm infection intensity which may in turn lead to an increased incidence of colic. Furthermore, it provides support to the hypothesis that the risk of ileal impaction colic and spasmodic colic increases with tapeworm infection intensity. The practical application of the anti‐12/13 kDa IgG(T) ELISA is demonstrated by this study.
Design and clinical use of a modified colon tray for large colon evacuation in the horse
Authors: MARIËN T. , ADRIAENSSEN A. , SEGERS L. 
Published: June 2010

SummaryA modified colon tray to perform large colon evacuations in horses was designed. A funnel‐shaped accessory was constructed to create a mechanical separation between the sterile and nonsterile part of the tray. Sterile hooks were used to make a temporary and stabile colostomy. Thirty horses with surgical correction of nonstrangulating large colon obstruction underwent a large colon evacuation with this modified tray. The surgical procedure was uncomplicated and resulted in a positive outcome in 29 horses. This less labour‐intensive way to evacuate the large colon results in minor contamination and makes extraluminal lavage becomes superfluous. This modified tray is a very efficacious and practical instrument for emptying the large colon in horses.
Equine lymphocytic‐plasmacytic enterocolitis: A retrospective study of 14 cases
Authors: KEMPER D. L. , PERKINS G. A. , SCHUMACHER J. , EDWARDS J. F. , VALENTINEN B. A. , DIVERS T. J. , COHEN N. D. 
Published: June 2010

SummaryLymphocytic‐plasmacytic enteritis (LPE) is a morphological diagnosis given to a type of infiltrative intestinal disease classified within the complex of idiopathic inflammatory bowel disease (IBD). The purpose of this retrospective study was to describe the details of breed, age and sex, clinical and clinicopathological findings and outcome of horses diagnosed with LPE. Data were reported from 14 horses that had a histopathological diagnosis of LPE; the median age was 12 years, and there was no breed or sex predilection. Common clinical signs were weight loss (100%), diarrhoea (50%) and lethargy (50%). Hypoproteinaemia and hypoalbuminaemia were present in 6/13 (46%) and 9/12 (75%) horses, respectively. Results of the oral glucose tolerance tests or D‐xylose absorption tests were abnormal in 9/12 (75%) horses and rectal mucosal biopsies were abnormal in 3/7 (43%) horses. Corticosteroid treatment was used unsuccessfully in 4 horses. All horses were subjected to euthanasia and LPE was diagnosed at necropsy. Diffuse thickening of the small intestine was apparent grossly in 10/13 (77%) horses examined. LPE is an uncommon equine intestinal disease that is difficult to diagnoseantemortemand has a poor prognosis.
Relationships between third metacarpal bone parameters and surface strains
Authors: DAVIES H. M. S. 
Published: June 2010

SummaryThe relationships were explored between measurements of bone quality, size (quantity) and shape, and strain data collected during treadmill exercise at 4 and 12 m/s from the third metacarpal bones of 6 yearling and 6 mature Thoroughbred racehorses. Peak strains in yearling and mature Thoroughbred horses during exercise were related to the size and shape of the third metacarpal bone. The peak strains were significantly lower in horses with a greater cross‐sectional area of bone and with a greater proportion of the bone in the dorsal cortex. There was no consistent relationship between peak strains and bone quality assessed by ultrasound speed, single photon absorptiometry and direct measurement of porosity and specific gravity. It was concluded that measurement of the dorsal cortical proportions of the third metacarpal bone may provide a way of estimating the level of strains caused by fast exercise in this part of the bone, and hence the modelling response to be expected.
Sensitivity of forelimb swing phase inverse dynamics to inertial parameter errors
Authors: LANOVAZ J. L. , CLAYTON H. M. 
Published: June 2010

SummaryEstimations of segmental inertial parameters are required for true inverse dynamics calculations during the swing phase of locomotion. This study attempts to quantify the effect of inertial parameter errors on inverse dynamic solutions. Swing phase forelimb net joint moments and powers at the trot (mean ± s.d 3.03 ± 0.16 m/s) were calculated with sagittal plane kinematic data from 5 Dutch Warmbloods using inertial parameters based on published regression equations for the breed. Significant peaks in the net moment and power curves for each forelimb joint were identified and measured. Net joint moments and powers were then recalculated after varying the segment mass, location of the segment centre of mass and the mass moment of inertia separately for each of the limb segments. Peak values for the net joint moments and joint powers were determined after each variation, and the percent change in peak value per percent change in inertial parameter was calculated. Segment mass was the most influential parameter, followed by location of the centre of mass. Changes in the mass moment of inertia showed little effect on peak values. The most influential single inertial parameter was the mass of the hoof segment with a mean ± s.d effect 0.74 ± 0.22 and 0.69 ± 0.18 percent peak change per percent parameter change on net joint moments and powers, respectively, across all joints. The results demonstrate the need for an accurate approximation of segment masses during the swing phase, especially the hoof, and the need to account for any additional masses in the model, such as shoes.
Finite element analysis (FEA) as a model to predict effects of farriery on the equine hoof
Authors: HINTERHOFER C. , STANEK C. , HAIDER H. 
Published: June 2010

SummaryA finite element (FE) hoof capsule was built as a small, symmetrical forelimb hoof on IDEAS* as a model for calculation and visualisation of stress and displacement of the equine hoof capsule. The model's loading was performed according to the suspension of the coffin bone within the hoof wall (pulling force) and over the sole and frog (compressing force) with a total of 3000 N. Restraints of the model's ground nodes and surface wall nodes were defined for simulation of 4 shoeing situations: a regular horseshoe, a horseshoe with a toe clip, a horseshoe with regular side clips and a horseshoe with a toe clip and more caudally‐placed side clips, all fixed to the hoof capsule with 3 nails on each side and each calculated in a tense and a loose nailed condition. Von Mises stresses were taken ranging from 1.22 N/mm2in the weighthearing border of the side clip shoe fixed loosely to the capsule up to 16.67 N/mm2in the hoof horn material surrounding the third nail. Further high stress zones were calculated in the proximal dorsal wall, the distal heel and the lateral hoof wall. Displacement values were taken showing movements of hoof wall, sole and frog according to the shoeing conditions. Maximal displacement was calculated in the hoof capsule shod with a regular horseshoe without a clip. Minimal displacement was found in the capsule with a toe clip and 2 side clips placed behind the 3rd nail. All models showed higher displacements when calculated with a loose nail fixation.Validation of the detailed features of the models is not yet possible. Finite element analysis (FEA) can be used practically to predict influences of various farrier techniques on the equine hoof in order to avoid possible harm to horses' feet in field studies.