bluuebunny/crossref_metadata_2025_split huggingface dataset:
Deracemisation of α‐Chiral Primary Amines by a One‐Pot, Two‐Step Cascade Reaction Catalysed by ω‐Transaminases
Authors: Koszelewski Dominik , Clay Dorina , Rozzell David , Kroutil Wolfgang 
Published: April 2009

AbstractRacemic α‐chiral primary amines were deracemised to optically pure amines in up to >99 % conversion and >99 % ee within 48 h. The deracemisation was a result of a stereoinversion of one amine enantiomer; the formal stereoinversion was achieved by a one‐pot, two‐step procedure: in the first step, kinetic resolution of the chiral racemic amine was performed by employing a ω‐transaminase to yield an intermediate ketone and the remaining optically pure amine; in the second step, the ketone intermediate was stereoselectively transformed into the amine by employing alanine as the amine donor and a ω‐transaminase displaying opposite stereopreference than the ω‐transaminase in the first step. In the second step, lactate dehydrogenase was used to remove the side product pyruvate to shift the unfavourable reaction equilibrium to the product side. Depending on the order of the enantiocomplementary enzymes employed in the cascade, the (R), as well as the (S), enantiomer was accessible.(© Wiley‐VCH Verlag GmbH & Co. KGaA, 69451 Weinheim, Germany, 2009)
Predictive index for carcinoma of thyroid nodules and its integration with fine‐needle aspiration cytology
Authors: Kuru Bekir , Gulcelik Nese Ersoz , Gulcelik Mehmet Ali , Dincer Halil 
Published: April 2009

AbstractBackgroundThe objective of this study was to select patients for resection of thyroid malignity among patients with thyroid nodules by integration of predictive indices with fine‐needle aspiration cytology (FNAC).MethodsCharacteristics of 571 euthyroid patients with thyroid nodules who underwent surgery in our institution were prospectively recorded. Predictive factors for malignancy were identified and categorized as predictive indices that were integrated with FNAC to select patients for surgery.ResultsEighty‐three (14.5%) of the 571 patients had thyroid carcinoma. Size ≥4 cm, age ≥65, cervical lymph nodes, solid structure, hypoechogenicity, microcalcification, and elevated serum thyroglobulin levels were independent predictive factors associated with thyroid malignancy. Sensitivity, specificity, and accuracy of FNAC were 88%, 80%, and 81%, respectively, and were 100% for index 3.ConclusionsPatients with malignant and suspicious FNAC findings and, among patients with follicular neoplasm and nondiagnostic FNAC findings, those with ≥2 risk factors should undergo surgery. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Growth inhibition of head and neck carcinomas by D‐allose
Authors: Mitani Tomoo , Hoshikawa Hiroshi , Mori Terushige , Hosokawa Tomohiro , Tsukamoto Ikuko , Yamaguchi Fuminori , Kamitori Kazuyo , Tokuda Masaaki , Mori Nozomu 
Published: April 2009

AbstractBackgroundAn inhibitory effect of D‐allose, a rare sugar, on several cancer cell lines has been reported. This study aimed to investigate the growth inhibition of head and neck squamous cell carcinoma cells by D‐allose.MethodsWe treated 3 head and neck carcinoma cell lines with D‐allose, D‐fructose, D‐psicose, and D‐glucose. Cell growth assays as well as analyses of messenger RNA (mRNA) expression, cell cycle, apoptosis, and uptake of 14C‐glucose were performed.ResultsD‐allose had inhibitory effects on all 3 cell lines and tended to upregulate mRNA expression of glucose transporters, p21 and p53, and downregulate mRNA expression of cyclin A2, cyclin B1, and CDC2. We observed that D‐allose tended to interfere with the intracellular uptake of D‐glucose and induced apoptosis.ConclusionOur results indicate that D‐allose inhibits the growth of head and neck squamous cell carcinoma cells. D‐allose has a considerable potential as a new anticancer agent in those patients. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Impact of treating facilities' volume on survival for early‐stage laryngeal cancer
Authors: Chen Amy Y. , Pavluck Alex , Halpern Michael , Ward Elizabeth 
Published: April 2009

AbstractBackgroundTreatment at a high‐volume facility has been associated with better outcomes in a variety of conditions. The relationship between volume and survival from laryngeal cancer has not been examined previously.MethodsA total of 11,446 early‐stage laryngeal cancer patients (1996–1998) who reported to the National Cancer Database (NCDB) were analyzed. Proportional hazards regression was used to assess the relationship between survival and treatment volume controlling for other factors associated with survival.ResultsTreatment at low‐volume facilities was associated with a significantly increased likelihood of death (hazard ratio 1.20, 95% CI 1.04–1.38). Surgical resection, as compared with radiation treatment, was associated with lower mortality (HR 0.74, 95% CI 0.69–0.80).ConclusionThis study is the first to assess the relationship between survival and treatment volume in laryngeal cancer. Treatment at a high‐volume facility is associated with better survival. Surgical treatment rather than radiation was also associated with better survival, although we could not control for confounders that may bias treatment selection. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Warthin tumor presenting as a fungal abscess in an immunocompetent host: Case report and review of the literature
Authors: Leibowitz Jason M. , Montone Kathleen T. , Basu Devraj 
Published: April 2009

AbstractBackgroundFungal abscesses of the parotid gland are rare, and cases arising within parotid neoplasms have not been described previously. This report conveys our experience managing such an entity, which is further distinguished by its occurrence in an immunocompetent host.Methods and ResultsA 59‐year‐old man experienced multiple recurrences of a parotid fungal abscess requiring repeated drainage procedures. Definitive excision ultimately demonstrated Candida glabrata infecting a Warthin tumor.ConclusionsThis case is the first report of a parotid neoplasm presenting as a fungal abscess. It contributes to the scant literature on fungal abscesses of the parotid, which previously has only been described in debilitated hosts and without an associated neoplasm. The case also expands the spectrum of disease associated with C. glabrata. Warthin tumor may be an occult etiology for a parotid fungal abscess, and definitive diagnosis and therapy may require parotidectomy. © 2009 Wiley Periodicals, Inc. Head Neck, 2010
Intensity‐modulated radiation therapy with simultaneous integrated boost technique following neoadjuvant chemotherapy for locoregionally advanced nasopharyngeal carcinoma
Authors: Kim Kyubo , Wu Hong‐Gyun , Kim Hak Jae , Sung Myung‐Whun , Kim Kwang Hyun , Lee Se‐Hoon , Heo Dae Seog , Kim Hee Jung , Park Charn 
Published: April 2009

AbstractBackgroundOur aim was to evaluate the feasibility and efficacy of intensity‐modulated radiation therapy (IMRT) with simultaneous integrated boost (SIB) technique following neoadjuvant chemotherapy for locoregionally advanced nasopharyngeal cancer (NPC).MethodsBetween April 2004 and December 2006, 25 patients with stage IIB to IVB NPC underwent 3 cycles of neoadjuvant chemotherapy followed by IMRT using SIB technique. Neoadjuvant chemotherapeutic regimens were 5‐fluorouracil and cisplatin in 11 patients; docetaxel, 5‐fluorouracil, and cisplatin in 8; and paclitaxel and cisplatin in 6. Dose prescription of IMRT was as follows: 67.5 Gy at 2.25 Gy/fraction to postchemotherapy gross tumor, 54 to 60 Gy at 1.8 to 2 Gy/fraction to subclinical disease, and 48 Gy at 1.6 Gy/fraction to elective neck. Seventeen patients received weekly cisplatin during the course of radiation therapy as well.ResultsWith a median follow‐up of 29 months for survivors (range, 14–39), the 3‐year local progression–free, regional progression–free, and distant metastasis–free survival rates were 89.6%, 87.2%, and 80.4%, respectively. Treatment was well tolerated despite the grade 3 mucositis (16%) and/or pharyngitis (16%). With follow‐up, the frequency of xerostomia decreased. At 3 months after IMRT, the proportions of Radiation Therapy Oncology Group grades 0, 1, and 2 xerostomia were 13%, 38%, and 50%, respectively. At 24 months, the corresponding figures were 36%, 46%, and 18%, respectively.ConclusionIMRT with SIB technique for locoregionally advanced NPC was feasible and effective regarding locoregional control and development of xerostomia, even after neoadjuvant chemotherapy. Definition of gross tumor volume by postchemotherapy extent of disease was also feasible. © 2009 Wiley Periodicals, Inc. Head Neck, 2009
Discharge and suspended sediment transport in the Ayeyarwady River, Myanmar: centennial and decadal changes
Authors: Furuichi T. , Win Z. , Wasson R. J. 
Published: April 2009

AbstractAmong the large rivers rising on the Tibetan Plateau and adjacent high mountains, the discharge and suspended sediment load of the Ayeyarwady (Irrawaddy) River are the least well known. Data collected between 1969 and 1996 at Pyay (Prome) are analysed to provide the best available modern estimate of discharge (379 ± 47 × 109 m3/year) and suspended sediment load (325 ± 57 × 106 t/year) for the river upstream of the delta head. A statistical comparison with data collected in the nineteenth century (1871 to 1879) shows discharge has significantly decreased in the last ∼100 years. Regression and correlation analyses between discharge in the modern period and indices of El Niño–Southern Oscillation (ENSO) show a relationship. Copyright © 2009 John Wiley & Sons, Ltd.
Redefining the American quilt: definitions and experiences of community among ethnically diverse lesbian and bisexual women
Authors: Lehavot Keren , Balsam Kimberly F. , Ibrahim‐Wells Gemma D. 
Published: April 2009

AbstractLesbian and bisexual women from diverse backgrounds possess unique viewpoints regarding the meanings and functions of “community.” Despite this, few studies have explored sexual minority women's understanding of and relationship to their communities. The present study employed qualitative research methods to investigate the meanings and functions of community among a sample of ethnically diverse lesbian and bisexual women (N=49). From the participants' responses in semistructured focus groups and key informant interviews, three core constructs emerged: community concepts (relevance, meaning), community‐based strain (racism, homophobia, biphobia, classism), and community‐based action (fluidity, exclusivity). Implications of these findings for future research and practice with this population of women will be discussed. © 2009 Wiley Periodicals, Inc.
Isolation and culture of primary bovine embryonic stem cell colonies by a novel method
Authors: Cao Shanbo , Wang Fang , Chen Zhisheng , Liu Zhong , Mei Cheng , Wu Haojia , Huang Junjiu , Li Chao , Zhou Lingjun , Liu Lin 
Published: April 2009

AbstractAuthentic bovine embryonic stem (ES) cell lines have not been established despite progress made for more than two decades. Isolation and culture of primary ES cell colonies are the first critical step towards establishment of stable ES cell lines. Here we report a novel method designated as “Separate and Seed” that contributes remarkably to efficient derivation of bovine primary ES‐like cell colonies from blastocysts. These primary cultured bovine ES‐like cells exhibit morphology typical of ES cells and express pluripotent molecular markers including Oct4, Nanog and alkaline phosphatase. Interestingly, bovine primary ES‐like cell colonies distinctively express both stage‐specific embryonic antigens 1 and 4 (SSEA1 and SSEA4), unlike mouse and human ES cells. These pluripotent markers may be used for characterization of authentic bovine ES cell lines in later studies. In contrast, whole embryos or inner cell mass (ICM) used for primary culture by conventional methods fails to produce primary bovine ES cell colonies that express all pluripotent stem cell markers shown above. Furthermore, bFGF improves growth and maintained undifferentiated state of bovine ES‐like cells for several passages, whereas LIF and ERK inhibitor PD98059 known to promote pluripotency of mouse ES cells are unable to sustain bovine ES‐like cells. Although continued efforts are required for improving long‐term culture of bovine ES cells, this novel “Separate and Seed” method provides an initial effective step that may eventually lead to derivation of authentic bovine ES cell lines. J. Exp. Zool. 311A:368–376, 2009. © 2009 Wiley‐Liss, Inc.
Histological and molecular analysis of the biceps tendon long head post‐tenotomy
Authors: Joseph Michael , Maresh Carl M. , McCarthy Mary Beth , Kraemer William J. , Ledgard Felicia , Arciero Cristina L. , Anderson Jeffrey M. , Nindl Bradley C. , Mazzocca Augustus D. 
Published: April 2009

AbstractTendinopathy is a vexing clinical problem as its onset and development is often asymptomatic and unrecognized until tendon rupture. While extensively studied in the rotator cuff, Achilles, and patellar tendons, no study to date has examined the histological and molecular characteristics of the tendinopathic biceps long‐head (LHB). The anatomy of the LHB is unique in that it comprises intra‐ and extra‐articular portions, each exposed to differing loading patterns. Eleven LHBs post‐tenotomy were sectioned, fixed in formalin, and stained (H&E; Alcian Blue), and gross structural organization of collagen measured using polarized light microscopy. Protein expression of intra‐ and extra‐articular portions of the tenotomized biceps for IGF‐I, collagen III, and MMP‐1, ‐2, ‐3, and ‐13 was determined with Western blot analyses. The intra‐articular LHB exhibited significantly greater histological evidence of tendinopathy inclusive of increased proteoglycan (p < 0.05) and decreased organization as measured by polarized light microscopy (p < 0.01). The intra‐articular LHB also had significantly increased expression of collagen type III (p < 0.01) and of MMP‐1 and 3 (p < 0.01, p < 0.05 respectively). No significant differences were found for IGF‐I or for MMP‐2 and ‐13. The intra‐articular LHB exhibited histological characteristics of tendinopathy. Protein expression of the intra‐articular LHB did not universally display signs of tendinopathy in comparison to the extra‐articular portion of the tendon. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27:1379–1385, 2009
Finite element simulation of cement‐bone interface micromechanics: A comparison to experimental results
Authors: Janssen Dennis , Mann Kenneth A. , Verdonschot Nico 
Published: April 2009

AbstractRecently, experiments were performed to determine the micromechanical behavior of the cement‐bone interface under tension‐compression loading conditions. These experiments were simulated using finite element analysis (FEA) to test whether the micromechanical response of the interface could be captured in micromodels. Models were created of experimental specimens based upon microcomputed tomography data, including the complex interdigitated bone‐cement morphology and simulated frictional contact at the interface. The models were subjected to a fully reversed tension‐compression load, mimicking the experimental protocol. Similar to what was found experimentally, the simulated interface was stiffer in compression than in tension, and the majority of displacement was localized to the cement‐bone interface. A weak correlation was found between the FEA‐predicted stiffness and the stiffness found experimentally, with average errors of 8 and 30% in tension and compression, respectively. The hysteresis behavior found experimentally was partially reproduced in the simulation by including friction at the cement‐bone interface. Furthermore, stress analysis suggested that cement was more at risk of fatigue failure than bone, concurring with the experimental observation that more cracks were formed in the cement than in the bone. The current study provides information that may help explain the load transfer mechanisms taking place at the cement‐bone interface. © 2009 Orthopaedic Research Society. Published by Wiley Periodicals, Inc. J Orthop Res 27:1312–1318, 2009
Vibrational studies of hexagonal bronze systems: phonon calculation and high pressure induced phase transformation
Authors: Coelho J. Santos , Paraguassu W. , Maczka M. , Souza Filho A. G. , Freire P.T.C. , Mendes Filho J. , Hanuza J. 
Published: April 2009

AbstractHexagonal bronzes and valence‐balanced hexatungstates (VBHT) have attracted great attention for presenting rich polymorphism and displaying superconductor or ferroelectric properties. In the present work, structural and vibrational properties of RbBi1/3W8/3O9 (RBW) crystal (VBHT type) were investigated by high‐pressure Raman scattering experiments. The results suggest the existence of a reversible pressure‐induced structural phase transition at about 4 GPa. This transformation is most likely related to rotations of octahedral units along the c‐axis with no abrupt changes of apical OWO bonds length. In order to get further understanding of RBW vibrational properties, we performed phonon calculations, by using classical lattice dynamics, in the related hexagonal structure of the K0.26WO6 system. These calculations revealed that most of vibrational properties of K0.26WO6 are governed by tubular ‘like’ vibrations of the hexagonal cavity, which resemble similar vibrations of tubular nanostructures. The pressure dependence of Raman modes is understood in terms of calculated phonon eigenvectors at ambient pressure. Copyright © 2009 John Wiley & Sons, Ltd.
A weak solution approach to a reaction–diffusion system modeling pattern formation on seashells
Authors: Kelkel Jan , Surulescu Christina 
Published: April 2009

AbstractWe investigate a reaction–diffusion system proposed by H. Meinhardt as a model for pattern formation on seashells. We give a new proof for the existence of a local weak solution for general initial conditions and parameters upon using an iterative approach. Furthermore, the solution is shown to exist globally for suitable initial data. The behavior of the solution in time and space is illustrated through numerical simulations. Copyright © 2009 John Wiley & Sons, Ltd.
Biochemie und Molekularbiologie 2008
Authors: Marienhagen Jan , Schwaneberg Ulrich 
Published: April 2009

AbstractUm Enzyme als effiziente Katalysatoren in chemischen Synthesen einzusetzen, sind häufig ihre Eigenschaften wie Lösungsmittelstabilitäten zu verbessern. Fortschritte in der Molekularbiologie insbesondere der gelenkten Enzymevolution und dem rationalen Enzymdesign helfen, das Synthesepotenzial von Enzymen auszuschöpfen. Die RNA‐Biochemie bleibt ein Treffpunkt interdisziplinärer Forschung. Mit Einzelmolekültechniken lassen sich die vielfältigen Funktionen der RNA untersuchen.
Chemiedidaktik 2008
Authors: Becker Hans‐Jürgen , Stäudel Lutz , Hildebrandt Henry 
Published: April 2009

AbstractDie Forschungsperspektive Diagnose ist in vierfacher Hinsicht zu präzisieren: Schülerverhalten, Lehrerverhalten, Lehrerausbildung, Schul‐ und Unterrichtsstruktur. Diese Bereiche sind als Ganzes zu sehen. Schließlich sind Konsequenzen im Sinne therapeutischer Maßnahmen abzuleiten und umzusetzen.
Dynamic mechanical properties of aluminum nitride/cyanate ester composites for high performance electronic packaging
Authors: Ling Wei , Gu Aijuan , Liang Guozheng , Yuan Li , Liu Jie 
Published: April 2009

AbstractViscoelastic ature is one of the key features of polymeric composites. A series of cyanate ester (CE)‐based composites with different aluminum nitride (AlN) contents for high performance electronic packaging, coded as AlN/CE, were developed; the viscoelastic nature of AlN/CE composites was intensively investigated by employing dynamic mechanical analysis (DMA). Results show that the AlN content has a great effect on dynamic mechanical properties of AlN/CE composites. The storage modulus in the glassy region increases linearly with the addition of AlN as well as the increase of AlN content. Meanwhile, all composites also exhibit notably higher loss modulus than cured CE resin due to the appearance of new energy dissipation forms. In addition, the incorporation of AlN has a significant effect on damping factor peak. All reasons leading to these phenomena are analyzed from the view of structure–property relationship. Copyright © 2009 John Wiley & Sons, Ltd.
In vitro evaluation of the ability of platelet‐rich plasma to seal an iatrogenic fetal membrane defect
Authors: Lewi L. , Liekens D. , Heyns L. , Poliard E. , Beutels E. , Deprest J. , Hoylaerts M. F. 
Published: April 2009

AbstractObjectivesThe purpose of the study is to evaluate the ability of platelet‐rich plasma (PRP) to seal an iatrogenic fetal membrane defect.MethodsFirst, we evaluated the stability of a PRP plug in an amniotic fluid environment. Further, we evaluated the sealing capability of PRP plugs in an in vitro model that mimics a fetoscopic membrane defect. Finally, we examined its influence on membrane repair and cell proliferation in monolayer cell cultures and amnion‐chorion tissue explants.ResultsPRP plugs persisted in an amniotic fluid for a median time of 7 weeks. PRP plugs also provided waterproof sealing of a fetoscopic membrane defect. Finally, PRP stimulated cell proliferation in a monolayer cell culture and provided a good matrix for cell proliferation and migration in amnion–chorion tissue explants.ConclusionOur in vitro experiments suggest that PRP plugs may provide a long‐lasting, waterproof sealing of fetal membrane defects and stimulate fetal membrane repair. Copyright © 2009 John Wiley & Sons, Ltd.
Introduction of Carboxyl Functional Groups onto Platinum by RF Plasma Deposition
Authors: Leber Elizabeth R. , Ratner Buddy D. 
Published: April 2009

AbstractRadio frequency (RF) plasma deposition of acrylic acid was used to generate carboxyl functional groups on a platinum surface serving as a model of a platinum electrode. The groups were retained at around 11% of the carbon content of the surface as measured using electron spectroscopy for chemical analysis (ESCA) following challenges of water soaks and acidic and basic conditions. Angle‐dependent ESCA showed the percentage of carboxyl groups decreased with depth and supported our hypothesis that the plasma deposited acrylic acid bonds to the platinum by penetrating into the uppermost layer of the platinum. The carboxyl groups were generated on the platinum surface in sufficient number to be used for further modification of the surface.magnified image
Thin Film Deposition in Capacitively Coupled Plasmas Fed with Bis(dimethylamino)dimethylsilane and Oxygen: An FTIR study
Authors: Di Mundo Rosa , Palumbo Fabio , Fracassi Francesco , d'Agostino Riccardo 
Published: April 2009

AbstractThis paper deals with the deposition of thin films in capacitively coupled RF plasmas fed with bis(dimethylamino)dimethylsilane in mixture with argon. The effect of input power and of O2 addition has been studied by means of a detailed FTIR investigation of the plasma phase and FTIR and XPS analyses of the deposits. High carbon content films with inorganic character are deposited in monomer‐Ar discharges at high power. Input power rise results in higher monomer depletion but fragmentation appears not complete since SiMe, SiN, NMe absorptions are still evident. When the oxygen addition is high, a SiO2‐like film is formed and the oxidation of the organic fraction appears directly correlated with CO2 density in gas phase.magnified image