bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pubic bone injuries in primiparous women: magnetic resonance imaging in detection and differential diagnosis of structural injury
Authors: Brandon C. , Jacobson J. A. , Low L. K. , Park L. , DeLancey J. , Miller J. 
Published: July 2011

AbstractObjectiveTo evaluate the utility of magnetic resonance imaging (MRI) in diagnosing structural injury in primiparous women at risk for pelvic floor injury.MethodsThis was an observational study of 77 women who underwent 3T MRI after delivery. Women were operationally defined as high risk (n = 45) for levator ani muscle tears (risk factors: second‐stage labor > 150 min or < 30 min, anal sphincter tear, forceps, maternal age > 35 years and birth weight > 4000 g) or low risk (n = 32): vaginally delivered without these risk factors (n = 12); delivered by Cesarean section after second‐stage labor > 150 min (n = 14) or delivered by Cesarean section without labor (n = 6). All women were imaged using fluid‐sensitive MRI sequences. Two musculoskeletal radiologists reviewed images for bone marrow edema, fracture, pubic symphysis measurements and levator ani tear.ResultsMRI showed pubic bone fractures in 38% of women at high risk for pelvic floor injury and in 13% of women at low risk for pelvic floor injury (χ2(3) = 9.27, P = 0.03). Levator ani muscle tears were present in 44% of the high‐risk women and in 9% of the low‐risk women (χ2(3) = 11.57, P = 0.010). Bone marrow edema in the pubic bones was present in 61% of women studied across delivery categories. Complex patterns of injury included combinations of bone marrow edema, fractures, levator ani tears and pubic symphysis injuries. No MRI‐documented injuries were present in 18% of women at high risk and 44% at low risk for pelvic floor injury (χ2(1) = 6.2, P = 0.013).ConclusionsCriteria identifying primiparous women at risk for pelvic floor injury can predict increased risk of bone and soft tissue changes at the pubic symphysis. Fluid‐sensitive MRI has utility for differential diagnosis of structural injury in postpartum women. Copyright © 2012 ISUOG. Published by John Wiley & Sons, Ltd.
A prospective analysis of pain experience, beliefs and attitudes, and pain management of a cohort of Danish surgical patients
Authors: Lorentzen V. , Hermansen I.L. , Botti M. 
Published: July 2011

AbstractBackgroundAdequacy of pain management is a process indicator of health care quality with consequences for patient outcomes and satisfaction. The reported incidence of moderate to severe postoperative pain worldwide is between 20% and 80%.ObjectivesThe purpose was to assess the quality of pain management in a cohort of Danish postoperative patients by examining their pain experience, beliefs about pain and pain treatment, and relationships between pain intensity, its effect on function, and pharmacological pain management.MethodsThe American Pain Society's Patient Outcome Questionnaire was administered to a consecutive cohort of Danish patients who had undergone gastrointestinal, gynaecological, orthopaedic or urological surgery within 24 and 72 h of surgery.ResultsFindings indicated uncontrolled pain in 45.5% of patients. These patients reported moderate to severe intensity average pain in the previous 24 h, however, 88.4% of the cohort overall stated they were satisfied or very satisfied with pain treatment. Patients who experienced severe pain only received 50% of available strong opioids, 73.3% of available weak opioids, 100% of available non‐steroidal antiinflammatory drugs (NSAIDS) and paracetamol. Further, analgesics prescribed to be administered at fixed intervals were administered 99% of the time; in contrast, all Pro Re Nata (PRN) orders irrespective of analgesic categories, were administered only 25% of the time.ConclusionsA number of patients experienced significant pain postoperatively. Although multi‐modal analgesics were available, analgesic administration practices did not consistently reflect management responsive to patient needs. Despite this, patients were largely satisfied with the care received suggesting the need for further research to understand how patients perceive the efficacy of pain management.
Maternal low-protein diet-induced delayed reflex ontogeny is attenuated by moderate physical training during gestation in rats
Authors: Falcão-Tebas Filippe , Bento-Santos Adriano , Antônio Fidalgo Marco , de Almeida Marcelus Brito , Antônio dos Santos José , Lopes de Souza Sandra , Manhães-de-Castro Raul , Góis Leandro Carol 
Published: July 2011

We evaluated the effects of moderate- to low-intensity physical training during gestation on reflex ontogeny in neonate rats whose mothers were undernourished. Virgin female Wistar rats were divided into four groups as follows: untrained (NT, n 7); trained (T, n 7); untrained with a low-protein diet (NT+LP, n 7); trained with a low-protein diet (T+LP, n 4). Trained rats were subjected to a protocol of moderate physical training on a treadmill over a period of 4 weeks (5 d/week and 60 min/d, at 65 % of VO2max). After confirming the pregnancy, the intensity and duration of the exercise were reduced. Low-protein groups were provided with an 8 % casein diet, and controls were provided with a 17 % casein diet. Their respective offspring were evaluated (during the 10th–17th days of postnatal life) in terms of physical feature maturation, somatic growth and reflex ontogeny. Pups born to mothers provided with the low-protein diet during gestation and lactation showed delayed physical feature and reflex maturation and a deficit in somatic growth when compared with controls. However, most of these deficiencies were attenuated in pups of undernourished mothers undergoing training. In conclusion, physical training during gestation attenuates the effects of perinatal undernutrition on some patterns of maturation in the central nervous system during development.
Comparison of novel and standard methods for analysing patterns of plant death in designed field experiments
Authors: SURIYAGODA L. D. B. , RYAN M. H. , LAMBERS H. , RENTON M. 
Published: July 2011

SUMMARYThe present paper compares standard and novel methods for analysing aggregated patterns of plant death in designed field experiments; these methods include binomial (BN), beta-binomial (BBN), logistic-normal-binomial (LNB), BN models with random blocks, BN models with smooth-scale spatial components and principal coordinates of neighbour matrices (PCNM). PCNM is a relatively new technique used in ecology to determine how much observed variability can be explained by spatial and environmental variables, and has not yet been applied to agricultural studies. The survival data of two pasture species, collected from a designed field experiment that was replicated at multiple locations, were used. First, the occurrence of overdispersion was tested using the BN and BBN distributions. Goodness-of-fit tests proved that the BBN model provided a better description (better fit) of the observed data in some cases than did the BN distribution, indicating overdispersion was present. When overdispersion was not present, the BN distribution was adequate to describe the data, and the use of the BBN distribution was superfluous. It is then shown that the PCNM approach, the BN model with smooth-scale spatial components and the LNB model were able to account for some of the variation as spatial variability, thus reducing the species effect compared with that explained under the standard BN model. The amount of variation among species according to the BN model and the BN model with random blocks was similar. Therefore, it is argued that the novel PCNM approach warrants further testing when exploring the spatial variability in designed experiments in agriculture and using LNB, PCNM and BN with smooth-scale spatial components may provide better predictions of species effects than do other, more conventional, approaches.
Ethnic Proximity and Ethnic Voting in Peru
Authors: MADRID RAÚL L. 
Published: July 2011

AbstractPeru, in contrast to neighbouring Bolivia and Ecuador, has neither an important indigenous party nor a strong indigenous movement. Nevertheless, in recent years a growing gap has emerged in the voting patterns of indigenous and non-indigenous areas. This article maintains that this gap has developed because some Peruvian politicians, including Alberto Fujimori, Alejandro Toledo and Ollanta Humala, successfully wooed indigenous voters with a combination of ethnic and populist appeals. Like traditional populist leaders, they denounced the political elites, focused their campaigns on the poor and presented themselves as the saviours of Peru, but also forged ties to indigenous leaders, invoked indigenous symbols and embraced some ethnic demands. Although neither Fujimori, nor Toledo, nor Humala self-identified as indigenous, they successfully presented themselves as more ethnically proximate to the indigenous population than their main competitors, who represented the white Lima elite.
COLLECTIVE MEMORY IN THE FRONTIERS: A CASE STUDY FROM THE ANCIENT MAYA CENTER OF MINANHA, BELIZE
Authors: Iannone Gyles 
Published: July 2011

AbstractThe examination of the internal frontiers between ancient Maya polities is a topic that has received little focused attention. This article explores various topics associated with frontiers and frontier communities, including: (1) how they might be located archaeologically; (2) what their material correlates might be; and, (3) what we might expect in terms of their sociopolitical characteristics. Special attention is paid to the role that collective memory plays in both the definition and reaffirmation of territorial limits and in the production and reproduction of frontier identities. The ancient Maya center of Minanha is used as a case study.
THE MYSTERIOUS AND THE INVISIBLE: WRITING HISTORY IN AND OF COLONIAL YUCATAN
Authors: Restall Matthew 
Published: July 2011

AbstractThis brief essay argues that studying the non-Spanish inhabitants of Yucatan's past requires bridging the social distance generated by differences of time and culture and that the specific nature of that distance must first be understood. With respect to the Mayas, their mystique in the modern popular and academic imaginations is as much the creation of Maya elites in ancient and colonial times as it is the product of archaeologists and historians. To demystify the Maya, we must engage mundane as well as exotic sources and be aware of the obfuscating influence of those who interpreted Maya culture before us. A complete picture of colonial Yucatan and of the colonial Mayas must include Afro-Yucatecans, or Africans and their descendents in the peninsula. Rendered invisible by historical processes and lack of scholarly attention, Afro-Yucatecans must be fully examined if we are to fully grasp the Yucatec experience, including the Yucatec Maya experience.
A MAZAPA PHASE COPPER FIGURINE FROM ATETELCO, TEOTIHUACAN: DATA AND SPECULATIONS
Authors: Hosler Dorothy , Cabrera Ruben 
Published: July 2011

AbstractExcavations of the Mazapan phase at the Atetelco complex at Teotihuacan by Ruben Cabrera and colleagues produced a small human copper figurine. Studies of Mesoamerican metallurgy have indicated that metallurgy was introduced to western Mexico arounda.d.700 from Colombia, and Ecuador. This particular figurine is a remarkable find. The dates (a.d.800–900) precede those of copper artifacts in the central Highlands. The manufacturing method (an open-backed casting) is rare in Mesoamerica but common in Colombia and Panama. Open-backed cast figurines that do appear in Mesoamerica come from the Cenote of Sacrifice, are made from copper-gold alloys, and were imported from lower Central America or Colombia. The Atetelco figurine is made from a copper-arsenic alloy. Copper-arsenic alloy objects are common in western Mexico (aftera.d.1150) and Ecuador and northern Peru (after abouta.d.300). The major question concerning the Atetelco figurine is whether it was fashioned in Ecuador or Colombia and imported, or whether it was produced in western Mexico. Our study of artifact chemistry, fabrication methods, and provenience indicate that the weight of the evidence points to western Mexico. We suggest that this fabrication technique (lost-wax castings of open-backed human figurines), while introduced from Colombia and lower Central America, never became a major component of the Mesoamerican metallurgical tradition.
Design and rationale of a genetic cohort study on congenital cardiac disease: experiences from a multi-institutional platform in Quebec
Authors: Dubé Marie-Pierre , Bigras Jean-Luc , Thibeault Maryse , Bureau Nathalie , Chetaille Philippe , Richter Andrea , Mercier Jocelyne , Bellavance Marc , Rohlicek Charles , Rozen Rima , Nemer Mona , Khairy Paul , Gendron Roxanne , Andelfinger Gregor 
Published: July 2011

AbstractBackgroundCongenital cardiac disease is the most common malformation, and a substantial source of mortality and morbidity in children and young adults. A role for genetic factors is recognised for these malformations, but overall few predisposing loci have been identified. Here we report the rationale, design, and first results of a multi-institutional congenital cardiac disease cohort, assembled mainly from the French-Canadian population of the province of Quebec and centred on families with multiple affected members afflicted by cardiac malformations.MethodsFamilies were recruited into the study, phenotyped and sampled for DNA in cardiology clinics over the first 3 years of enrolment. We performed segregation analysis and linkage simulations in the subgroup of families with left ventricular outflow tract obstruction (LVOTO).ResultsA total of 1603 participants from 300 families were recruited, with 169 out of 300 (56.3%) families having more than one affected member. For the LVOTO group, we estimate heritability to be 0.46–0.52 in our cohort. Simulation analysis demonstrated sufficient power to carry out linkage analyses, with an expected mean log-of-odds (LOD) score of 3.8 in 67 pedigrees with LVOTO.ConclusionWe show feasibility and usefulness of a population-based biobank for genetic investigations into the causes of congenital cardiac disease. Heritability of LVOTO is high and could be accounted for by multiple loci. This platform is ideally suited for multiple analysis approaches, including linkage analysis and novel gene sequencing approaches, and will allow to establish segregation of risk alleles at family and population levels.
New York Heart Association class assessment by cardiologists and outpatients with congenital cardiac disease: a head-to-head comparison of three patient-based versions
Authors: Schoormans Dounya , Mager Yuma L. , Oort Frans J. , Sprangers Mirjam A.G. , Mulder Barbara J.M. 
Published: July 2011

AbstractBackgroundThe objective of this study was to compare three patient-based New York Heart Association assessments with cardiologist assessments in outpatients with congenital cardiac disease.MethodsConsecutive adult outpatients completed three questionnaires in a random order: a patient-based translation of the New York Heart Association classes, a self-constructed questionnaire based on the New York Heart Association classes, and the Specific Activity Scale. The treating cardiologist assessed the New York Heart Association class on the same day. Patient–cardiologist agreement was assessed by calculating percent agreement and weighted kappa. We also explored the level of agreement for patients without co-morbidity.ResultsIn all, 86 adults – with a median age of 35.8 years – including 46 women participated. An agreement of 75.6% (weighted kappa is 0.43; probability is smaller than 0.01), 70.6% (weighted kappa is 0.44; probability is smaller than 0.01), and 74.4% (weighted kappa is 0.28; probability is smaller than 0.01) was found between the cardiologist assessment and the patient-based translation, self-constructed questionnaire, and the Specific Activity Scale, respectively. The patient-based translation equally over- and underestimated the New York Heart Association class, whereas the self-constructed questionnaire overestimated and the Specific Activity Scale underestimated the New York Heart Association class. Agreement levels for patients without co-morbidity were higher than agreement levels for the total group.ConclusionThe patient-based translation yielded adequate agreement with cardiologist-assessed New York Heart Association class, showed equal over- and underestimation, and was easy to complete. The patient-based translation with the instruction to only consider functional impairments caused by the congenital cardiac defect is recommended in future studies of outpatients with congenital cardiac disease.
Hospital Emergency Readiness Overview Study
Authors: Kollek Daniel , Cwinn A. Adam 
Published: July 2011

AbstractIn 2001, a survey of Canadian emergency departments indicated significant deficiencies in disaster preparedness. Since then, there have been efforts on the part of Provincial governments to remedy this situation. This survey repeats the original study with minor modifications to determine if there has been improvement. The Hospital Emergency Readiness Overview study demonstrates that despite improvements, there remain gaps in Canadian healthcare facility readiness for disaster, specifically one involving contaminated patients. It also highlights the lack of any standardized assessment of healthcare facilities' chemical, biological, radiological, or nuclear readiness.
Ceramide synthase 4 and de novo production of ceramides with specific N-acyl chain lengths are involved in glucolipotoxicity-induced apoptosis of INS-1 β-cells
Authors: Véret Julien , Coant Nicolas , Berdyshev Evgeny V. , Skobeleva Anastasia , Therville Nicole , Bailbé Danielle , Gorshkova Irina , Natarajan Viswanathan , Portha Bernard , Le Stunff Hervé 
Published: July 2011

Pancreatic β-cell apoptosis induced by palmitate requires high glucose concentrations. Ceramides have been suggested to be important mediators of glucolipotoxicity-induced β-cell apoptosis. In INS-1 β-cells, 0.4 mM palmitate with 5 mM glucose increased the levels of dihydrosphingosine and dihydroceramides, two lipid intermediates in the de novo biosynthesis of ceramides, without inducing apoptosis. Increasing glucose concentrations to 30 mM amplified palmitate-induced accumulation of dihydrosphingosine and the formation of (dihydro)ceramides. Of note, glucolipotoxicity specifically induced the formation of C18:0, C22:0 and C24:1 (dihydro)ceramide molecular species, which was associated with the up-regulation of CerS4 (ceramide synthase 4) levels. Fumonisin-B1, a ceramide synthase inhibitor, partially blocked apoptosis induced by glucolipotoxicity. In contrast, apoptosis was potentiated in the presence of D,L-threo-1-phenyl-2-palmitoylamino-3-morpholinopropan-1-ol, an inhibitor of glucosylceramide synthase. Moreover, overexpression of CerS4 amplified ceramide production and apoptosis induced by palmitate with 30 mM glucose, whereas down-regulation of CerS4 by siRNA (short interfering RNA) reduced apoptosis. CerS4 also potentiates ceramide accumulation and apoptosis induced by another saturated fatty acid: stearate. Collectively, our results suggest that glucolipotoxicity induces β-cell apoptosis through a dual mechanism involving de novo ceramide biosynthesis and the formation of ceramides with specific N-acyl chain lengths rather than an overall increase in ceramide content.
The Arabidopsis phi class glutathione transferase AtGSTF2: binding and regulation by biologically active heterocyclic ligands
Authors: Dixon David P. , Sellars Jonathan D. , Edwards Robert 
Published: July 2011

The plant-specific phi class of glutathione transferases (GSTFs) are often highly stress-inducible and expressed in a tissue-specific manner, suggestive of them having important protective roles. To date, these functions remain largely unknown, although activities associated with the binding and transport of reactive metabolites have been proposed. Using a sensitive and selective binding screen, we have probed the Arabidopsis thaliana GSTFs for natural product ligands from bacteria and plants. Uniquely, when overexpressed in bacteria, family members GSTF2 and GSTF3 bound a series of heterocyclic compounds, including lumichrome, harmane, norharmane and indole-3-aldehyde. When screened against total metabolite extracts from A. thaliana, GSTF2 also selectively bound the indole-derived phytoalexin camalexin, as well as the flavonol quercetin-3-O-rhamnoside. In each case, isothermal titration calorimetry revealed high-affinity binding (typically Kd&lt;1 μM), which was enhanced in the presence of glutathione and by the other heterocyclic ligands. With GSTF2, these secondary ligand associations resulted in an allosteric enhancement in glutathione-conjugating activity. Together with the known stress responsiveness of GSTF2 and its association with membrane vesicles, these results are suggestive of roles in regulating the binding and transport of defence-related compounds in planta.
ATF4 deficiency protects mice from high-carbohydrate-diet-induced liver steatosis
Authors: Li Houkai , Meng Qingshu , Xiao Fei , Chen Shanghai , Du Ying , Yu Junjie , Wang Chunxia , Guo Feifan 
Published: July 2011

Chronic feeding of HCD (high-carbohydrate diet) is one of the major contributors to the prevailing of metabolic diseases. ATF4 (activating transcription factor 4) has been shown to play an important role in the regulation of glucose metabolism and obesity development; however, it is unclear how ATF4−/− mice respond to HCD. In the present study, we show that 8 weeks of HCD results in significant higher accumulation of TAGs (triacylglycerols) in livers and impairment in glucose tolerance in ATF4+/+ mice, but not in ATF4−/− mice, compared with those on a normal diet. Meanwhile, energy expenditure is further enhanced by HCD in ATF4−/− mice. Moreover, we show that ATF4 deficiency suppresses HCD-induced SCD1 (stearoyl-CoA desaturase 1) expression, furthermore, oral supplementation of the main product of SCD1 oleate (18:1) increases TAG accumulation in livers of ATF4−/− mice. Taken together, these results suggest that ATF4 deficiency is protective for HCD-induced hepatic steatosis and impairment of glucose tolerance and insulin sensitivity. Furthermore, the resistance to hepatic steatosis is at least in part due to suppression of SCD1 expression under HCD.
Reconstitution in liposomes of the functionally active human OCTN1 (SLC22A4) transporter overexpressed in Escherichia coli
Authors: Pochini Lorena , Scalise Mariafrancesca , Galluccio Michele , Amelio Linda , Indiveri Cesare 
Published: July 2011

The hOCTN1 (human organic cation transporter 1) overexpressed in Escherichia coli and purified by Ni-chelating chromatography has been reconstituted in liposomes by detergent removal with a batch-wise procedure. The reconstitution was optimized with respect to the protein concentration, the detergent/phospholipid ratio and the time of incubation with Amberlite XAD-4 resin. Time-dependent [14C]tetraethylammonium, [3H]carnitine or [3H]ergothioneine uptake was measured in proteoliposomes with activities ratios of 8:1.3:1 respectively. Optimal activity was found at pH 8.0. The transport depended on intraliposomal ATP. [14C]tetraethylammonium transport was inhibited by several compounds. The most effective were acetyl-choline and γ-butyrobetaine, followed by acetylcarnitine and tetramethylammonium. Reagents such as pyridoxal 5-phosphate, MTSES [sodium (2-sulfonatoethyl) methanethiosulfonate] and mercurials strongly inhibited the transport. From kinetic analysis of tetraethylammonium transport a Km of 0.77 mM was calculated. Acetylcholine and γ-butyrobetaine behaved as competitive inhibitors of TEA (tetraethylammonium) transport with Ki values of 0.44 and 0.63 mM respectively.