bluuebunny/crossref_metadata_2025_split huggingface dataset:
Telemedical Interventional Monitoring in Heart Failure (TIM‐HF), a randomized, controlled intervention trial investigating the impact of telemedicine on mortality in ambulatory patients with heart failure: study design
Authors: Koehler Friedrich , Winkler Sebastian , Schieber Michael , Sechtem Udo , Stangl Karl , Böhm Michael , Boll Herbert , Kim Simone S. , Koehler Kerstin , Lücke Stephanie , Honold Marcus , Heinze Peter , Schweizer Thomas , Braecklein Martin , Kirwan Bridget‐Anne , Gelbrich Goetz , Anker Stefan D. ,   on behalf of the TIM‐HF Investigators
Published: November 2010

AimsRemote patient management (telemonitoring) may help to detect early signs of cardiac decompensation, allowing optimization of and adherence to treatments in chronic heart failure (CHF). Two meta‐analyses have suggested that telemedicine in CHF can reduce mortality by 30–35%. The aim of the TIM‐HF study was to investigate the impact of telemedical management on mortality in ambulatory CHF patients.MethodsCHF patients [New York Heart Association (NYHA) II/III, left ventricular ejection fraction (LVEF) ≤35%] with a history of cardiac decompensation with hospitalization in the past or therapy with intravenous diuretics in the prior 24 months (no decompensation required if LVEF ≤ 25%) were randomized 1:1 to an intervention group of daily remote device monitoring (electrocardiogram, blood pressure, body weight) coupled with medical telephone support or to usual care led by the patients’ local physician. In the intervention group, 24/7 physician‐led medical support was provided by two central telemedical centres. A clinical event committee blinded to treatment allocation assessed cause of death and reason for hospitalization. The primary endpoint was total mortality. The first secondary endpoint was a composite of cardiovascular mortality or hospitalization due to heart failure. Other secondary endpoints included cardiovascular mortality, all‐cause and cause‐specific hospitalizations (all time to first event) as well as days lost due to heart failure hospitalization or cardiovascular death (in % of follow‐up time), and changes in quality of life and NYHA class. Overall, 710 CHF patients were recruited. The mean follow‐up was 21.5 ± 7.2 months, with a minimum of 12 months.PerspectiveThe study will provide important prospective outcome data on the impact of telemedical management in patients with CHF.
Novel X‐linked inhibitor of apoptosis inhibiting compound as sensitizer for TRAIL‐mediated apoptosis in chronic lymphocytic leukaemia with poor prognosis
Authors: Frenzel Lukas P. , Patz Michaela , Pallasch Christian P. , Brinker Reinhild , Claasen Julia , Schulz Alexandra , Hallek Michael , Kashkar Hamid , Wendtner Clemens‐Martin 
Published: November 2010

SummaryGiven that aggressive DNA damaging chemotherapy shows suboptimal efficacy in chronic lymphocytic leukaemia (CLL), alternative therapeutic approaches are needed. Tumour necrosis factor‐related apoptosis‐inducing ligand (TRAIL) is able to induce tumour‐specific apoptosis. However, apoptosis might be inhibited by elevated levels of X‐linked inhibitor of apoptosis (XIAP). Use of XIAP‐inhibiting compounds might sensitize primary CLL cells towards TRAIL‐mediated apoptosis. A novel small molecule, compound A (CA), an inhibitor of XIAP, was used in combination with TRAIL to induce apoptosis in primary CLL cells (n = 48). XIAP was significantly more highly expressed in primary CLL cells (n = 28) compared to healthy B cells (n = 16) (P = 0·02). Our data obtained by specific knock‐down of XIAP by siRNA identified XIAP as the key factor conferring resistance to TRAIL in CLL. Combined treatment with CA/TRAIL significantly increased apoptosis compared to untreated (P = 8·5 × 10−10), solely CA (P = 4·1 × 10−12) or TRAIL treated (P = 4·8 × 10−10) CLL cells. CA rendered 40 of 48 (83·3%) primary CLL samples susceptible to TRAIL‐mediated apoptosis. In particular, cells derived from patients with poor prognosis CLL (ZAP‐70+, IGHV unmutated, 17p‐) were highly responsive to this drug combination. Our highly‐effective XIAP inhibitor CA, in concert with TRAIL, shows potential for the treatment of CLL cases with poor prognosis and therefore warrants further clinical investigation.
Effect of storage on resistant starch content and in vitro starch digestibility of some pressure‐cooked cereals and legumes commonly used in India
Authors: Yadav Baljeet S. , Sharma Alka , Yadav Ritika B. 
Published: November 2010

SummaryResistant starch (RS) is that fraction of starch, which escapes enzymic hydrolysis in the small intestine and passes in the colon. Effect of storage time (12 and 24 h) and temperature (4 °C and 25 °C) was studied on RS content of the pressure‐cooked cereal and legume grains/seeds and their flours. RS content was observed to increase in the stored cereals and legumes, with more enhanced increase in the flour samples stored at refrigeration temperature for longer duration (41.4% in wheat flour and 85.4% in pea flour). Significant positive correlations were observed between RS content (4 °C, 24 h) and amylose (y = 0.388 × –5.948, r = 0.840, P ≤ 0.05, n = 7) as well as between % increase in insoluble dietary fibre content (4 °0C, 24 h) and amylose (y = 2.257 × –27.724, r = 0.971, P ≤ 0.05, n = 7). Reduced in vitro starch digestibility of the cooked/stored samples (4 °C, 24 h) was observed when compared to freshly cooked samples.
Isolation of probiotic bacteria from turmeric (Curcuma longa Linn.) and its application in enriched beverages
Authors: Pianpumepong Plangpin , Noomhorm Athapol 
Published: November 2010

SummaryThe purpose of this study is to obtain lactic acid bacteria (LAB) from fresh turmeric rhizomes that could be used as starter culture with the ability to ferment turmeric and to investigate its influence on total phenolic content (TPC) and antioxidant activity. Three LAB strains, namely Enterococcus faecium, Lactococcus lactis subsp. lactis, and Lactobacillus plantarum, were isolated from turmeric rhizomes. These microorganisms showed similar characteristics as probiotic organisms. The TPC of fermented turmeric beverages was measured using the Folin–Denis reagent method, while the antioxidant activity assays were quantified by 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) and ferric reducing antioxidant power assay. This study confirms that bacteria isolated from turmeric can be used as starter. Moreover, fermentation time and LAB lead to dominant amount of TPC and that they have high levels of antioxidant activities.
A PCR assay to detect trace amounts of soybean in meat sausages
Authors: Soares Sónia , Mafra Isabel , Amaral Joana S. , Oliveira Maria Beatriz P.P. 
Published: November 2010

SummarySoybean proteins are the most widely used source vegetable proteins in the meat industry because of several interesting characteristics. As soybean is included in the group of ingredients potentially allergenic, if not declared, it can be considered a hidden allergen, representing a potential risk to sensitised individuals. The aim of this work was to optimise and apply DNA‐based techniques for soybean detection in meat products, as alternative to the currently used protein‐based methods. The optimised polymerase chain reaction (PCR) protocol targeting the soybean lectin gene enabled the detection of the addition of 0.1% and 0.5% of hydrated textured protein, which corresponded to 0.01% and 0.06% (w/w) of soybean protein in unprocessed and heat‐processed pork meats, respectively. The established PCR technique, when applied to commercial meat sausages (eighteen samples), confirmed the presence of soybean declared in nine samples and indicated the presence of soybean in four samples with no labelled information about soybean. Additionally, the event‐specific PCR detection of Roundup Ready® soybean was also performed, enabling the detection of transgenic DNA in three samples.
Variation among physical, chemical and technological properties in three Sicilian cultivars of Chickpea (Cicer arietinum L.)
Authors: Avola Giovanni , Patanè Cristina 
Published: November 2010

SummaryThree Sicilian chickpea cultivars (‘Calia’, ‘Etna’ and ‘Principe’) were evaluated for physical, chemical and technological properties. Whereas no substantial differences were ascertained on seeds chemical composition, the cultivars greatly differed in terms of seed size, specific surface area (SSA) and seed coat incidence. These last two properties affected the technological properties of the seeds. In particular, a high SSA (‘Calia’) contributed to a faster softening, whilst a great coat thickness (‘Etna’) delayed both hydration and softening rate. The time required to obtain an acceptable firmness (cooking time) was greatly reduced by presoaking the seeds in salt solution (0.5% NaCl or NHCO3). ‘Calia’ required the lowest cooking time when soaked in distilled water or in 0.5% NaCl. Presoaking seed in NaHCO3 allowed halving the cooking time in all cultivars.
Preparation, digestibility, and glucose response in mice of rice coated with resistant starch type 4 using locust bean gum and agar
Authors: Choi Hea Jin , Lee Chang Joo , Cho Eun Ju , Choi Seung Jun , Moon Tae Wha 
Published: November 2010

SummaryThis study investigated the technical feasibility of coating type 4 resistant starch (RS4) onto rice with the edible polymers locust bean gum (LBG) and agar. The coating solutions were prepared using LBG, agar, and a mixture of LBG and agar at three concentrations (0.01%, 0.1% and 1%). The raw milled rice was soaked in the prepared coating solutions, rolled in RS4 powder and dried. Microscopic observation revealed that the RS4 coating on the rice surface with 0.1% and 1% LBG–agar mixture had a resistance to washing and cooking and covered the whole surface of the rice with good adherence. The thickness of the RS4 coating on the rice surface with 0.1% and 1% LBG–agar mixture was approximately 74 and 233 μm, respectively. Compared with uncoated rice and rice mixed with RS4, the RS4‐coated rice showed lower starch digestibility, a decreased glucose response and a slower rate of blood glucose decrease.
Influence of the degree of fusariosis on technological traits of wheat grain
Authors: Siuda Ryszard , Grabowski Andrzej , Lenc Leszek , Ralcewicz Maria , Spychaj‐Fabisiak Ewa 
Published: November 2010

SummaryThe paper aims to enlarge the current knowledge about the dependence of technological traits of wheat grain on its degree of infestation (DI) with fusariosis. Samples of grain of four wheat cultivars were obtained from naturally grown crops (control) and from crops inoculated with Fusarium culmorum from which three degrees of kernel infestation were selected. Samples were determined for thousand kernels weight (TKW), total protein, starch and wet gluten (WG) contents, Hagberg falling number (HFN) and sedimentation value (SV). Content of protein and wet gluten showed higher values for moderately infested kernels (up to 15 and 25%, respectively, when compared to control grain) than for kernels lightly or heavily infested. All the remaining traits had values lower than that for control grain and showed a decrease with increasing DI. Decrease for the heaviest infestation was on average: 8, 29, 31 and 34% for starch, TKW, HFN and SV, respectively.
Development of peanut milk–based fermented curd
Authors: Yadav Deep N. , Singh Krishan K. , Bhowmik Subrato N. , Patil Ramabhau T. 
Published: November 2010

SummaryFermented milk product (Indian curd) was developed by using peanut milk. The level of incubation period (IP), skimmed milk powder (SMP), carboxymethyl cellulose (CMC) was optimised using response surface methodology (RSM). Central composite rotatable design was used with three independent variables i.e. IP (16–20 h), SMP (3–5%) and CMC (0.1–0.3%). SMP had significant (P ≤ 0.05) positive effect on overall acceptability (OAA) of curd because of increase in the total solids that ultimately increased firmness of the curd. CMC was found effective in reducing the synersis of curd samples. The developed curd samples had moisture 84.8 ± 0.28%, protein 3.2 ± 0.12%, fat 3.5 ± 0.10%, ash 0.5 ± 0.05%, carbohydrate 8.0% (wb), peak viscosity 291.4 ± 3.52 cP, firmness 1.3 ± 0.15 N, synersis 32.1 ± 0.2 mL 100 g−1 and acidity (as % lactic acid) 0.58 ± 0.02. It had OAA score of 7.8 ± 0.2 on nine‐point hedonic scale. Based on compromise optimisation, the conditions recommended were IP as 18 h, SMP, 4.24% and CMC, 0.19% for making peanut milk–based fermented curd with 83.4% desirability.
Edible coating affects physico‐functional properties and shelf life of chicken eggs during refrigerated and room temperature storage
Authors: Wardy Wisdom , Torrico Damir D. , No Hong Kyoon , Prinyawiwatkul Witoon , Saalia Firibu K. 
Published: November 2010

SummaryEffects of chitosan, whey protein concentrate (WPC), mineral oil (MO) and/or soybean oil (SO) coating on egg quality were compared at 25 and 4 °C, respectively, during 5 and 20 weeks of storage. Storage time and temperature, and type of coating significantly affected Haugh unit, yolk index, weight loss, albumen pH and emulsifying capacity. Shelf life was extended 4 weeks by MO and SO and 2 weeks by chitosan and WPC longer than that observed for noncoated eggs at 25 °C. MO‐ and SO‐coated eggs maintained AA grade for 20 weeks at 4 °C. Weight loss of SO‐coated eggs was <1% after 5 weeks at 25 °C and after 20 weeks at 4 °C. Yolk index and emulsifying capacity were more correlated at 25 °C than at 4 °C. MO and SO were more effective coating materials, with SO providing a more cost‐effective coating for extending egg shelf life.
Optimisation of a model food mixture using response surface methodology to evaluate the anti‐adhesive properties of cooking materials
Authors: Zorita Saioa , Niquet Céline , Bonhoure Jean‐Paul , Robert Nathalie , Tessier Frédéric J. 
Published: November 2010

SummaryThe composition of a model food was optimised to evaluate the anti‐adhesive properties of cooking materials. This optimisation was performed using a response surface methodology. When cooked at 201 °C, the model food was selected to leave the greatest quantity of cooked residue that cannot be removed easily from the utensil surface. The measurement of the surface covered by the cooked food left after cleaning was the response value of the experimental design. The effect of five macronutrients was evaluated. Glucose and ovalbumin factors showed the most important positive effect, while amylopectin presented a negative effect. The optimum concentration values of the food constituents where highest food adsorption was estimated were 34.5 mg mL−1 glucose, 14.5 mg mL−1 amylopectin, 39.0 mg mL−1 ovalbumin, 13.5 mg mL−1 casein and 32.8 mg mL−1 linoleic acid. The predicted results at the optimum point coincided well with experimental values. The optimum food mixture presented good precision as well.
Prospects for person‐centred diagnosis in general medicine
Authors: Klinkman Michael , van Weel Chris 
Published: November 2010

AbstractFundamental changes in health care delivery are revealing the limitations of our collective focus on disease‐specific and technology‐driven health care. We increasingly treat diseases, not persons, and it moves individuals from active participants in the care process to passive recipients of interventions. This problem is especially important for general medicine, where we must maintain the balance between person and disease, caring and technology. In this chapter, we focus on prospects for person‐centred diagnosis and treatment in general (primary care) medicine in this time of change. We describe one way to employ the biopsychosocial model to integrate person‐centred diagnosis in routine clinical practice, and we propose a ‘person‐centred path’ for primary care development with the health information technology tools we will need to develop to follow that path.
A systematic approach for uptake of evidence on sex‐specific issues in guidelines – a pilot study
Authors: Keuken Debby , Bindels Patrick , Klazinga Niek , Haafkens Joke 
Published: November 2010

AbstractRationale, aims and objectives  Increasing evidence indicates that sex‐specific issues may have impact on prevention, diagnosis, or treatment. These issues are not systematically considered during the development of Dutch clinical practice guidelines. The aim of this study is to identify how members of guideline development groups discuss sex‐specific evidence, and whether and how the outcomes of these discussions are reflected in the guideline.Methods  Six guideline development committees (GDCs) were studied. Each committee was supported by a staff member from the guideline organization who was trained and received feedback to facilitate uptake of evidence on sex differences in the process of guideline development. Non‐participant observation and transcription of audio recordings from 22 GDC meetings were performed. Content analysis of meeting transcripts and guidelines were studied to analyse characteristics of discussion episodes on sex‐specific research data‐based issues (subject matter, initiator and group approach towards the topic and themes) and whether or not conclusions on evidence were reflected in the final guideline text.Results  Of the 87 identified discussion episodes, 68 dealt with sex‐specific research evidence potentially relevant to guidelines. Respectively 51%, 28% and 21% of the latter episodes were initiated by committee members, staff members and chairpersons. Group approaches towards the subject matter were generally positive. Data from 60% of those episodes were reflected in the final guideline text. Sex‐specific data on reproductive issues were more often discussed and reflected in guideline texts than data on other health issues. Discussion episodes on sex‐specific evidence initiated by chairpersons were most often reflected in the guidelines.Conclusions  This pilot study indicates that GDCs regularly focused on sex‐specific issues. The participation of a trained staff member contributed to this.
Simple and direct detection of Aeromonas hydrophila infection in the goldfish, Carassius auratus (L.), by dot blotting using specific monoclonal antibodies
Authors: Longyant S , Chaiyasittrakul K , Rukpratanporn S , Chaivisuthangkura P , Sithigorngul P 
Published: November 2010

AbstractA combination of eight isolates of Aeromonas hydrophila was used to produce monoclonal antibodies (MAbs). Ten different groups of MAbs specific to Aeromonas were selected. The first five groups of MAbs demonstrated high specificity and bound to only one or two isolates of A. hydrophila. The sixth and the seventh groups of MAbs were A. hydrophila specific. They recognized seven of eight A. hydrophila isolates (AH1, 2, 3, 4, 5, 6, 8); however, the MAb in the seventh group also showed cross‐reactivity to one isolate of Aeromonas caviae (AC3). The eighth MAb group recognized two isolates of A. hydrophila (AH2 and AH5) and demonstrated cross‐reactivity to one isolate of Aeromonas sobria (AS1) and one isolate of A. caviae (AC3). The tenth group of MAbs bound to all isolates of Aeromonas spp. tested (AH1‐8, AS1‐6, AC1‐5, Aeromonas veronii and Aeromonas jandaei) without cross‐reactivity to any of the other bacteria tested. MAbs in the ninth group showed similar specificity to those in the tenth group but did not recognize two isolates of A. sobria (AS4 and AS6) or A. jandaei. All the MAbs could be used to identify Aeromonas by dot blotting with a sensitivity ranging from 105 to 107 CFU mL−1. However, the sensitivity of detection was increased to 102–103 CFU mL−1 after inoculation of the sample in tryptic soy broth for 3–6 h before performing the dot blotting. The dot blot method can be used for the direct detection of A. hydrophila infection in symptomatic and asymptomatic goldfish. This study demonstrated a convenient immunological tool that can be used for the direct detection of A. hydrophila and Aeromonas infections in a complex sample without the requirement for separation of the bacteria or isolation and biochemical tests.
Dietary fatty acids and inflammation in the vertebral column of Atlantic salmon, Salmo salar L., smolts: a possible link to spinal deformities
Authors: Gil Martens L , Lock E J , Fjelldal P G , Wargelius A , Araujo P , Torstensen B E , Witten P E , Hansen T , Waagbø R , Ørnsrud R 
Published: November 2010

AbstractVegetable oils (Vo) are an alternative to fish oil (Fo) in aquaculture feeds. This study aimed to evaluate the effect of dietary soybean oil (Vo diet), rich in linoleic acid, and of dietary fish oil (Fo diet) on the development of spinal deformities under bacterial lipopolysaccharide (LPS)‐induced chronic inflammation conditions in Atlantic salmon, Salmo salar L. Fish [25 g body weight (BW)] were fed the experimental diets for 99 days. On day 47 of feeding (40 g BW), fish were subjected to four experimental regimes: (i) intramuscular injections with LPS, (ii) sham‐injected phosphate‐buffered saline (PBS), (iii) intraperitoneally injected commercial oil adjuvant vaccine, or (iv) no treatment. The fish continued under a common feeding regime in sea water for 165 more days. Body weight was temporarily higher in the Vo group than in the Fo group prior to immunization and was also affected by the type of immunization. At the end of the trial, no differences were seen between the dietary groups. The overall prevalence of spinal deformities was approximately 14% at the end of the experiment. The Vo diet affected vertebral shape but did not induce spinal deformities. In groups injected with LPS and PBS, spinal deformities ranged between 21% and 38%, diet independent. Deformed vertebrae were located at or in proximity to the injection point. Assessment of inflammatory markers revealed high levels of plasma prostaglandin E2 (PGE2) in the Vo‐fed and LPS‐injected groups, suggesting an inflammatory response to LPS. Cyclooxigenase 2 (COX‐2) mRNA expression in bone was higher in fish fed Fo compared to Vo‐fed fish. Gene expression of immunoglobulin M (IgM) was up‐regulated in bone of all LPS‐injected groups irrespective of dietary oil. In conclusion, the study suggests that Vo is not a risk factor for the development of inflammation‐related spinal deformities. At the same time, we found evidence that localized injection‐related processes could trigger the development of vertebral body malformations.
Morphology and distribution of granulomatous inflammation in freshwater ornamental fish infected with mycobacteria
Authors: Novotny L , Halouzka R , Matlova L , Vavra O , Bartosova L , Slany M , Pavlik I 
Published: November 2010

AbstractMycobacteriosis in fish is a chronic progressive ubiquitous disease caused by Mycobacterium marinum, M. gordonae and M. fortuitum in most cases. The aim of this study was to describe the morphology and distribution of lesions in 322 freshwater ornamental fish across 36 species. Granulomatous inflammation was diagnosed by gross examination and histopathology testing in 188 fish (58.4%); acid‐fast rods (AFR) were determined in only 96 (51.1%) fish from 19 species after Ziehl–Neelsen staining. The most often affected organs with AFR were the kidney (81.2%), digestive tract (54.1%), liver (48.2%), spleen (45.9%) and skin (21.2%); sporadically, AFR were found in the branchiae (9.4%) and gonads (4.7%). In 14 randomly selected fish originating from four different fish tanks, the distribution of mycobacterial infection was studied by culture examination of the skin, gills, muscle tissue, digestive tract, liver, spleen and kidney. In 12 fish, the species M. marinum, M. gordonae, M. fortuitum, M. triviale, and M. avium subsp. hominissuis (serotypes 6 and 8 and genotype IS901− and IS1245+) were detected; mixed infection caused by different mycobacterial species was documented in five of them.