bluuebunny/crossref_metadata_2025_split huggingface dataset:
Constructions sociales et professionnelles de la sexualité dans le contexte de la maladie d'alzheimer
Authors: Giami Alain , Ory Lorraine 
Published: April 2012

Une analyse de la littérature scientifique et professionnelle sur le thème « sexualité et Alzheimer » a été réalisée afin d’appréhender les représentations savantes et professionnelles de la sexualité dans le champ de la maladie d’Alzheimer. Peu traité, ce thème est centré autour de la question des « comportements sexuels inappropriés », dont notamment « l’hypersexualité » qu’il s’agit de contenir, voire même de réduire. Ces comportements apparaissent comme une source de malaise pour les soignants, lorsqu’ils y sont confrontés dans leur pratique, ainsi que pour l’entourage familial.
Construire un outil de contrôle au sein des organisations publiques : une opportunité au développement d’un nouveau mode d’action
Authors: Dreveton Benjamin 
Published: April 2012

A partir d’une étude de cas, menée en collaboration avec une agence française (l’Agence de l’Environnement et de la Maîtrise de l’Energie) sur une période de deux ans, cette recherche s’intéresse au rôle joué par les acteurs de la construction d’un outil de calcul des coûts. En associant le processus de création d’un outil de gestion à un processus d’innovation managériale, cette recherche fait émerger un facteur de succès de l’implantation des outils de contrôle au sein des organisations publiques : la nécessaire création et stabilisation d’un nouvel espace social.
One‐Dimensional SDS Gel Electrophoresis of Proteins
Authors: Gallagher Sean R. 
Published: April 2012

AbstractOne‐dimensional gel electrophoresis of proteins provides information about the molecular size, amount, and purity of a protein sample. Separated proteins can be recovered from polyacrylamide gels for subsequent characterization by a variety of secondary techniques, such as mass spectrometry to determine post‐translational modifications and the amino acid sequence. In addition, one‐dimensional electrophoresis is the standard first step in immunoblotting and immunodetection. Protein separations in vertical slab gels are performed in a variety of formats. Most recently, small format minigels are typical due to their ease of use, low relative cost, and ready commercial availability. Larger gels provide more separation area and thus better resolution for complex samples and continue to be in use for specialized analysis. Curr. Protoc. Protein Sci. 68:10.1.1‐10.1.44. © 2012 by John Wiley & Sons, Inc.
Use of CID/ETD Mass Spectrometry to Analyze Glycopeptides
Authors: Mechref Yehia 
Published: April 2012

AbstractCollision‐induced dissociation (CID) tandem mass spectrometry (MS/MS) does not allow the characterization of glycopeptides because of the fragmentation of glycan structures and limited fragmentation of peptide backbones. Electron transfer dissociation (ETD) MS/MS, on the other hand, offers a complementary approach, prompting only peptide backbone fragmentation while keeping post‐translational modifications intact. Characterization of glycopeptides using both CID and ETD is summarized in this unit. While CID provides information related to the composition of glycan moieties attached to a peptide backbone, ETD permits de novo sequencing of peptides. Radical anion transfer of electrons to the peptide backbone in ETD induces cleavage of the N‐Cα bond. The glycan moiety is retained on the peptide backbone, largely unaffected by the ETD process, thus allowing the identification of the amino acid sequence of a glycopeptide and its glycosylation site. This unit discusses the use of both CID and ETD for better characterization of glycopeptides. Curr. Protoc. Protein Sci. 68:12.11.1‐12.11.11. © 2012 by John Wiley & Sons, Inc.
Overview of Protein Folding Mechanisms: Experimental and Theoretical Approaches to Probing Energy Landscapes
Authors: Morris Elizabeth R. , Searle Mark S. 
Published: April 2012

AbstractWe present an overview of the current experimental and theoretical approaches to studying protein folding mechanisms, set against current models of the folding energy landscape. We describe how stability and folding kinetics can be determined experimentally and how this data can be interpreted in terms of the characteristic features of various models from the simplest two‐state pathway to a multi‐state mechanism. We summarize the pros and cons of a range of spectroscopic methods for measuring folding rates and present a theoretical framework, coupled with protein engineering approaches, for elucidating folding mechanisms and structural features of folding transition states. A series of case studies are used to show how experimental kinetic data can be interpreted in the context of non‐native interactions, populated intermediates, parallel folding pathways, and sequential transition states. We also show how computational methods now allow transient species of high energy, such as folding transition states, to be modeled on the basis of experimental ϕ‐value analysis derived from the effects of point mutations on folding kinetics. Curr. Protoc. Protein Sci. 68:28.2.1‐28.2.22. © 2012 by John Wiley & Sons, Inc.
Culturing Embryonic Nasal Explants for Developmental and Physiological Study
Authors: Klenke Ulrike , Taylor‐Burds Carol 
Published: April 2012

AbstractPrimary cultures obtained from embryonic nasal placodes can maintain olfactory neurons, olfactory ensheathing cells, and large numbers of gonadotropin releasing hormone‐1 (GnRH) neurons. Depending on the age of the starting material, one can examine cell interactions important for placode formation or neuronal migration and axonal outgrowth. When generated at E11.5 in mouse, neuronal migration and axon outgrowth away from the main tissue mass occurs. This area of the explant, the periphery, is only a few cells thick. This characteristic offers the opportunity to image single cells and axons and allows pharmacological and molecular manipulations as well as physiological recordings to be performed. Here, we describe a system for culturing nasal explants used in our laboratory. This model system provides a method for obtaining physiological cellular responses with post hoc immunohistochemistry, or gene expression studies, on cells arising from the nasal placode. Curr. Protoc. Neurosci. 59:3.25.1‐3.25.16. © 2012 by John Wiley & Sons, Inc.
A Protein Cross‐Linking Assay for Measuring Cell Surface Expression of Glutamate Receptor Subunits in the Rodent Brain After In Vivo Treatments
Authors: Boudreau Amy C. , Milovanovic Mike , Conrad Kelly L. , Nelson Christopher , Ferrario Carrie R. , Wolf Marina E. 
Published: April 2012

AbstractTrafficking of neurotransmitter receptors between intracellular and cell surface compartments is important for regulating neurotransmission. We developed a method for determining if an in vivo treatment has altered receptor distribution in a particular region of rodent brain. After the treatment, brain slices are rapidly prepared from the region of interest. Then, cell surface–expressed proteins are covalently cross‐linked using the membrane‐impermeable, bifunctional cross‐linker bis(sulfosuccinimidyl)suberate (BS3). This increases the apparent molecular weight of surface receptors, while intracellular receptors are not modified. Thus, surface and intracellular receptor pools can be separated and quantified using SDS‐PAGE and immunoblotting. This method is particularly useful for analyzing AMPA receptor subunits, offering advantages in accuracy, efficiency, and cost compared to biotinylation. A disadvantage is that some antibodies no longer recognize their target protein after cross‐linking. We have used this method to quantify changes in receptor distribution after acute and chronic exposure to psychomotor stimulants. Curr. Protoc. Neurosci. 59:5.30.1‐5.30.19. © 2012 by John Wiley & Sons, Inc.
Diet‐Induced Models of Obesity (DIO) in Rodents
Authors: Bagnol Didier , Al‐Shamma Hussien A. , Behan Dominic , Whelan Kevin , Grottick Andrew J. 
Published: April 2012

AbstractObesity results from a complex interplay between a susceptible genotype and an environment that both promotes increased caloric intake and enables sustained decreases in energy expenditure. One commonly employed approach to modeling obesity in preclinical species is the diet‐induced obese (DIO) rodent. Here, theoretical and practical considerations for producing obese rodents via dietary manipulation, and for assessing drug‐induced changes in food intake and body weight are described. Based on these considerations, a standardized protocol for diet‐induced obesity in both mouse and rat is provided and sample data from these models are also described. Curr. Protoc. Neurosci. 59:9.38.1‐9.38.13. © 2012 by John Wiley & Sons, Inc.
Near‐Infrared Molecular Probes for In Vivo Imaging
Authors: Zhang Xuan , Bloch Sharon , Akers Walter , Achilefu Samuel 
Published: April 2012

AbstractCellular and tissue imaging in the near‐infrared (NIR) wavelengths between 700 and 900 nm is advantageous for in vivo imaging because of the low absorption of biological molecules in this region. This unit presents protocols for small animal imaging using planar and fluorescence lifetime imaging techniques. Included is an overview of NIR fluorescence imaging of cells and small animals using NIR organic fluorophores, nanoparticles, and multimodal imaging probes. The development, advantages, and application of NIR fluorescent probes that have been used for in vivo imaging are also summarized. The use of NIR agents in conjunction with visible dyes and considerations in selecting imaging agents are discussed. We conclude with practical considerations for the use of these dyes in cell and small animal imaging applications. Curr. Protoc. Cytom. 60:12.27.1‐12.27.20. © 2012 by John Wiley & Sons, Inc.
Rheumatoid arthritis disease activity measures: American College of Rheumatology recommendations for use in clinical practice
Authors: Anderson Jaclyn , Caplan Liron , Yazdany Jinoos , Robbins Mark L. , Neogi Tuhina , Michaud Kaleb , Saag Kenneth G. , O'dell James R. , Kazi Salahuddin 
Published: April 2012

AbstractObjectiveAlthough the systematic measurement of disease activity facilitates clinical decision making in rheumatoid arthritis (RA), no recommendations currently exist on which measures should be applied in clinical practice in the US. The American College of Rheumatology (ACR) convened a Working Group (WG) to comprehensively evaluate the validity, feasibility, and acceptability of available RA disease activity measures and derive recommendations for their use in clinical practice.MethodsThe Rheumatoid Arthritis Clinical Disease Activity Measures Working Group conducted a systematic review of the literature to identify RA disease activity measures. Using exclusion criteria, input from an Expert Advisory Panel (EAP), and psychometric analysis, a list of potential measures was created. A survey was administered to rheumatologists soliciting input. The WG used these survey results in conjunction with the psychometric analyses to derive final recommendations.ResultsSystematic review of the literature resulted in identification of 63 RA disease activity measures. Application of exclusion criteria and ratings by the EAP narrowed the list to 14 measures for further evaluation. Practicing rheumatologists rated 9 of these 14 measures as most useful and feasible. From these 9 measures, the WG selected 6 with the best psychometric properties for inclusion in the final set of ACR‐recommended RA disease activity measures.ConclusionWe recommend the Clinical Disease Activity Index, Disease Activity Score with 28‐joint counts (erythrocyte sedimentation rate or C‐reactive protein), Patient Activity Scale (PAS), PAS‐II, Routine Assessment of Patient Index Data with 3 measures, and Simplified Disease Activity Index because they are accurate reflections of disease activity; are sensitive to change; discriminate well between low, moderate, and high disease activity states; have remission criteria; and are feasible to perform in clinical settings.
A Versatile Synthetic Platform for a Wide Range of Functionalized Biomaterials
Authors: You Zhengwei , Wang Yadong 
Published: April 2012

AbstractThe introduction of functionalities to synthetic biomaterials represents a major direction and a significant challenge in biomedical engineering. A synthetic platform using novel acid‐induced epoxide ring‐opening polymerization promoted by a newly designed catalyst to produce a variety of biodegradable and functionalized biomaterials is reported. The polymerization proceeds smoothly in the presence of functional groups including alkenyl, aromatic, ether, ester, and free hydroxyl groups. The functionalities of the resultant biomaterials can be further enriched using many post‐functionalization methods. This platform yields biomaterials with a wide range of hydrophilicity, crystallinity, charge, mechanical properties, and cell interactions. This platform for functionalized biomaterials is simple, versatile, and can be easily adapted to specific cell types and tissues of interest.
Flexible Hybrid Paper Made of Monolayer Co3O4 Microsphere Arrays on rGO/CNTs and Their Application in Electrochemical Capacitors
Authors: Yuan Changzhou , Yang Long , Hou Linrui , Li Jiaoyang , Sun Yaxin , Zhang Xiaogang , Shen Laifa , Lu Xiangjun , Xiong Shenglin , Lou Xiong Wen (David) 
Published: April 2012

AbstractA facile one‐step hydrothermal method is developed for large‐scale production of well‐designed flexible and free‐standing Co3O4/reduced graphene oxide (rGO)/carbon nanotubes (CNTs) hybrid paper as an electrode for electrochemical capacitors. Densely packed unique Co3O4 monolayer microsphere arrays uniformly cover the surface of the rGO/CNTs film. The alkaline hydrothermal treatment leads to not only the deposition of Co3O4 microspheres array, but also the reduction of the GO sheets at the same time. The unique hybrid paper is evaluated as an electrode for electrochemical capacitors without any ancillary materials. It is found that the obtained hybrid flexible paper, composed of Co3O4 microsphere array anchored to the underling conductive rGO/CNTs substrate with robust adhesion, is able to deliver high specific capacitance with excellent electrochemical stability even at high current densities, suggesting its promising application as an efficient electrode material for electrochemical capacitors.
The Development of Hydrazine‐Processed Cu(In,Ga)(Se,S)2 Solar Cells
Authors: Bob Brion , Lei Bao , Chung Choong‐Heui , Yang Wenbing , Hsu Wan‐Ching , Duan Hsin‐Sheng , Hou William Wei‐Jen , Li Sheng‐Han , Yang Yang 
Published: April 2012

AbstractThe hydrazine‐based deposition of Cu(In,Ga)(S,Se)2 (CIGS) thin films has attracted considerable attention in recent years due to its potential for the high‐throughput production of photovoltaic devices based on this absorber material. This article provides an introduction as well as presenting a complete picture of the current status of hydrazine‐based CIGS solar‐cell fabrication, including the three major steps of this deposition process: dissolution of the precursor materials in hydrazine, deposition of a film from the resulting precursor solution, and the completion and characterization of a photovoltaic device following absorber deposition. Recent discoveries are then discussed, regarding the dissolution chemistry of the relevant precursor complexes in hydrazine, which together represent the true foundation of this processing method. Recent studies on CIGS film formation are then summarized, including the control and analysis of the crystalline phase, electronic bandgap, and film morphology. Finally, the latest progress in high‐performance device fabrication is highlighted, with a focus on optoelectronic characterization including current–voltage, junction capacitance, and minority carrier lifetime measurements. Finally, a discussion and future outlook is provided.
High Thermoelectric Figure of Merit in PbTe Alloys Demonstrated in PbTe–CdTe
Authors: Pei Yanzhong , LaLonde Aaron D. , Heinz Nicholas A. , Snyder G. Jeffrey 
Published: April 2012

AbstractPbTe alloys have been the most efficient materials for thermoelectric power generation since the 1950s. In addition to the observed lattice thermal conductivity reduction by alloy scattering, recent efforts targeting electronic‐transport enhancement through band structure engineering have demonstrated a design route to achieve extraordinary thermoelectric performance. Guided by these recent results, the current work discusses the influence of alloying CdTe in p‐type PbTe where the solubility is strongly temperature‐dependent. Beneficial changes to the band structure and effective scattering of phonons are concurrently inferred when the PbTe–CdTe precipitate composite is expected to become a solid solution at high temperature, enhancing the peak thermoelectric figure of merit to ~1.7. This work emphasizes the importance of solid solution alloys of PbTe for thermoelectric power‐generation applications and also provides an upper limit of about 1 micrometer to the average distance between interfaces above which phonon scattering by composite interfaces is ineffectual.
Attenuation of vagal recovery during sleep and reduction of cortisol/melatonin ratio in late afternoon associate with prolonged daytime sleepiness among media workers with irregular shift work
Authors: Lindholm Harri , Sinisalo Juha , Ahlberg Jari , Hirvonen Ari , Hublin Christer , Partinen Markku , Savolainen Aslak 
Published: April 2012

AbstractBackgroundMedia work is characterized by information flow, deadlines, and 24/7 alertness. Good recovery prevents stress‐related disorders.MethodsThe standardized questionnaire included items about health, health habits, sleep, work conditions, and work stress. Recordings of 24‐hr heart rate variability (HRV) and four salivary samples for cortisol and melatonin levels were analyzed from 70 randomly selected workers with irregular shift work, and 70 workers with normal daytime work.ResultsIrregular shift work increased the risk of insufficient recovery when compared to normal daytime work (OR 2.0; P < 0.05). In the group of workers with insufficient subjective recovery, HRV was attenuated (P < 0.05) during the early hours of night, and cortisol/melatonin ratio was decreased (P < 0.05) in the afternoon.ConclusionsPhysiological changes underlying subjective feelings of insufficient recovery are measurable. Attenuated HRV during sleep reflects prolonged sympathetic drive and/or impaired parasympathetic recovery. Interactions between cortisol and melatonin hormones might be involved in the development of chronic exhaustion. Am. J. Ind. Med. 55:643–649, 2012. © 2012 Wiley Periodicals, Inc.
Preparation and characterization of a novel once‐daily formulation of diltiazem using arabinogalactan as a channeling agent
Authors: Avramoff Avi , Khan Wahid , Mizrahi Boaz , Domb Abraham J. 
Published: April 2012

AbstractThe aim of the present study was to develop a once‐daily, delayed controlled release formulation for diltiazem. Developed formulation consists of two coated tablets inserted into a capsule; the first tablet is intended to produce a fast release profile, while the second tablet with a unique controlling membrane containing arabinogalactan as a channeling agent was designed to achieve a delayed controlled release profile for diltiazem. The in vitro characteristic of formulation was determined in terms of the surface morphology of the coated tablet, and the impact of the different polysaccharide in coating on the dissolution and in vitro drug release. Arabinogalactan was found to be most appropriate channeling agent to control in vitro drug release. When tested in buffer (pH 6.8) the formulation produced a desired delayed controlled release dissolution profile for over 24 h. Surface morphology of the coating film clearly demonstrated channeling formation on contact with the media. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Preparation and characterization of new organosoluble polyamides with good thermal properties containing photosensitive group in the main chain
Authors: Hajibeygi Mohsen , Shabanian Meisam 
Published: April 2012

AbstractIn this article, we have successfully synthesized a new dicarboxylic acid, from the two step reactions. At first 4,4′‐bis(1,2‐diphenoxyethane) dialdehyde was synthesized from 1,2‐dibromoethane and 4‐hydroxybenzaldehyde , then 4,4′‐bis(1,2‐diphenoxyethane) diacrylic acid was synthesized from 4,4′‐bis(1,2‐diphenoxyethane) dialdehyde and malonic acid in a solvent free reaction. Four new organosoluble and thermally stable polyamides (PAs) with good inherent viscosities containing photosensitive groups were synthesized from the direct polycondensation reaction of 4,4′‐bis(1,2‐diphenoxyethane) diacrylic acid with four various aromatic diamines containing photosensitive group, by two different methods such as direct polycondensation in a medium consisting of N‐methyl‐2‐pyrrolidone (NMP)/triphenyl phosphite (TPP)/calcium chloride (CaCl2)/pyridine (Py) and direct polycondensation in a tosyl chloride (TsCl)/pyridine (Py)/N,N‐dimethylformamide (DMF) system. All of the above polymers were fully characterized by 1H‐NMR, FTIR, gel permeation chromatography (GPC), elemental analysis, inherent viscosity, solubility tests, UV–vis spectroscopy, differential scanning calorimeter (DSC), thermogravimetric analysis (TGA). The resulted polyamides (PAs) have showed admirable good inherent viscosities, thermal stability and solubility. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012
Surfactant‐modified feldspar: Isotherm, kinetic, and thermodynamic of binary system dye removal
Authors: Yazdani Maryam , Mahmoodi Niyaz Mohammad , Arami Mokhtar , Bahrami Hajir 
Published: April 2012

AbstractIn this article, surface modification of feldspar using hexadecyltrimethyl ammonium bromide (HDTMA) and its dye removal ability in single and binary systems was studied. Acid Black 1 (AB1) and Acid Red 14 (AR14) were used as model dyes. The monocomponent Langmuir isotherm model was applied to experimental data and the isotherm constants were calculated for both dyes. The monolayer coverage capacities of surfactant‐modified feldspar (HDTMA‐feldspar) for AB1 and AR14 dyes in single solution system were found as 6.369 mg/g and 3.984 mg/g, respectively. It was observed that the equilibrium uptake amounts of AB1 and AR14 dye in binary mixture onto sorbent decreased with increasing concentrations of the other dye resulting in their antagonistic effect. Equilibrium adsorption for binary systems was analyzed by using the Extended Langmuir and Jain and Snoeyink Modified Extended Langmuir models. The rate of kinetic processes of single and binary dye systems onto adsorbent was described by using two kinetics adsorption models. The pseudo‐second‐order model was the best choice among the kinetic models to describe the adsorption behavior of single and binary dyes onto HDTMA‐feldspar. Thermodynamic parameters showed that dye adsorption on HDTMA‐feldspar were exothermic and unspontaneous in nature. © 2012 Wiley Periodicals, Inc. J Appl Polym Sci, 2012