bluuebunny/crossref_metadata_2025_split huggingface dataset:
Enhanced cellular therapy: revolutionizing adoptive cellular therapy
Authors: Xu Meng-Yao , Zeng Na , Liu Chen-Qian , Sun Jian-Xuan , An Ye , Zhang Si-Han , Xu Jin-Zhou , Zhong Xing-Yu , Ma Si-Yang , He Hao-Dong , Hu Jia , Xia Qi-Dong , Wang Shao-Gang 
Published: April 2024

AbstractEnhanced cellular therapy has emerged as a novel concept following the basis of cellular therapy. This treatment modality applied drugs or biotechnology to directly enhance or genetically modify cells to enhance the efficacy of adoptive cellular therapy (ACT). Drugs or biotechnology that enhance the killing ability of immune cells include immune checkpoint inhibitors (ICIs) / antibody drugs, small molecule inhibitors, immunomodulatory factors, proteolysis targeting chimera (PROTAC), oncolytic virus (OV), etc. Firstly, overcoming the inhibitory tumor microenvironment (TME) can enhance the efficacy of ACT, which can be achieved by blocking the immune checkpoint. Secondly, cytokines or cytokine receptors can be expressed by genetic engineering or added directly to adoptive cells to enhance the migration and infiltration of adoptive cells to tumor cells. Moreover, multi-antigen chimeric antigen receptors (CARs) can be designed to enhance the specific recognition of tumor cell-related antigens, and OVs can also stimulate antigen release. In addition to inserting suicide genes into adoptive cells, PROTAC technology can be used as a safety switch or degradation agent of immunosuppressive factors to enhance the safety and efficacy of adoptive cells. This article comprehensively summarizes the mechanism, current situation, and clinical application of enhanced cellular therapy, describing potential improvements to adoptive cellular therapy.
Effects of capture and GPS-tagging in spring on migration timing and reproduction in Pink-footed geese Anser brachyrhynchus
Authors: Schreven Kees H. T. , Madsen Jesper , Nolet Bart A. 
Published: April 2024

Abstract
Background
GPS-transmitters enable detailed study of animal behaviour but may impact the animals. Impacts vary from short-term stress and habituation to longer-term effects on e.g., migration and reproduction. To study impacts, ideally, true controls (i.e., uncaptured or untagged animals) are used, but unbiased assessments of their migration timing and breeding performance are challenging, especially in remote areas. Alternatively, quasi-controls can be used: individuals tagged longer ago, or the same tagged individuals but in later years. Quasi-controls reveal tagging effects that differ between the first and following years.

Results
We captured Pink-footed geese (Anser brachyrhynchus) in spring and summer and deployed GPS-transmitter neckbands. In spring, geese were caught with cannon or clap nets on stopovers in Norway and Finland, 2 weeks before departure to breeding areas in Svalbard and Novaya Zemlya. In summer, geese were rounded up during wing moult in Svalbard. First, we compared geese tagged recently in spring with geese tagged in spring or summer 1–4 years prior. Newly tagged geese migrated significantly later, by 2 days, than previously tagged geese, both at departure from the spring stopover and arrival to the breeding grounds, while migration duration did not differ. Breeding propensity and laying date did not differ, but nesting success tended to be lowered, resulting in a significantly lower annual probability to produce hatchlings in recently tagged geese than in previously tagged geese. Second, within individuals tagged in spring, spring migration advanced in their next year, suggesting delay in their first spring. This was likely not an ageing effect, as geese tagged in summer showed no advancing spring migration timing over the years. Third, in Svalbard, observed brood sizes of geese tagged in summer and untagged geese did not differ 1 year after tagging.

Conclusions
The capture and GPS-tagging of geese 2 weeks before spring departure delayed their spring migration and lowered their probability to produce hatchlings in that year. These effects lasted longer than previously reported week-long effects of GPS-tagging on time budgets in summer and of neck-banding on spring body condition. Additional study is needed to evaluate longer-term or permanent effects which remain undetected with quasi-controls.
Internet-based eHealth technology for emotional well-being among the older adults with a family cancer history: full mediation effects of health information self-efficacy and cancer fatalism
Authors: Lai Yuyuan Kylie , Ye Jizhou Francis , Ran Qiawen , Ao Harris Song 
Published: April 2024

Abstract
Background
Older adults with a family cancer history (FCH) face an increased cancer risk, which may adversely impact their emotional well-being. Internet-based eHealth technologies (IETs) provide a potential solution to this challenge. This study examines the influence of using IETs on the emotional well-being of older adults with FCH. It also delves into the mediating pathways through health information self-efficacy and cancer fatalism.

Methods
This study conducted a mediation analysis using data from the Health Information National Trends Survey (HINTS 6) collected from March 2022 to November 2022, focusing on older adults with FCH who had previously searched for cancer-related information (N = 1,280).

Results
In the mediation model, no positive direct associations between IETs usage and emotional well-being were found. Only health information self-efficacy and cancer fatalism were found to mediate the relationship between IETs usage and emotional well-being serially (β = 0.007, 95% CI [0.003, 0.012]).

Conclusions
The findings inform health information professionals and healthcare practitioners on enhancing the impact of IETs usage on individual health information self-efficacy, which mitigates cancer fatalism, contributing to better emotional well-being in the digital era.
What drives wild pig (Sus scrofa) movement in bottomland and upland forests?
Authors: Evans Tyler S. , Ellison Natasha , Boudreau Melanie R. , Strickland Bronson K. , Street Garrett M. , Iglay Raymond B. 
Published: April 2024

Abstract
Background
The wild pig (Sus scrofa) is an exotic species that has been present in the southeastern United States for centuries yet continues to expand into new areas dominated by bottomland and upland forests, the latter of which are less commonly associated with wild pigs. Here, we aimed to investigate wild pig movement and space use attributes typically used to guide wild pig management among multiple spatiotemporal scales. Our investigation focused on a newly invaded landscape dominated by bottomland and upland forests.

Methods
We examined (1) core and total space use using an autocorrelated kernel density estimator; (2) resource selection patterns and hot spots of space use in relation to various landscape features using step-selection analysis; and (3) daily and hourly differences in movement patterns between non-hunting and hunting seasons using generalized additive mixed models.

Results
Estimates of total space use among wild pigs (n = 9) were smaller at calculated core (1.2 ± 0.3 km2) and 90% (5.2 ± 1.5 km2) isopleths than estimates reported in other landscapes in the southeastern United States, suggesting that wild pigs were able to meet foraging, cover, and thermoregulatory needs within smaller areas. Generally, wild pigs selected areas closer to herbaceous, woody wetlands, fields, and perennial streams, creating corridors of use along these features. However, selection strength varied among individuals, reinforcing the generalist, adaptive nature of wild pigs. Wild pigs also showed a tendency to increase movement from fall to winter, possibly paralleling increases in hard mast availability. During this time, there were also increases in anthropogenic pressures (e.g. hunting), causing movements to become less diurnal as pressure increased.

Conclusions
Our work demonstrates that movement patterns by exotic generalists must be understood across individuals, the breadth of landscapes they can invade, and multiple spatiotemporal scales. This improved understanding will better inform management strategies focused on curbing emerging invasions in novel landscapes, while also protecting native natural resources.
Exploring the provenance of a Byzantine excavated assemblage of textile and leather finds by the application of instrumental analysis
Authors: Margariti Christina , Sava Gabriela , Vanden Berghe Ina , Filiou Daphne 
Published: April 2024

Abstract
Stereomicroscopy, Scanning Electron Microscopy-Energy Dispersive Spectroscopy (SEM–EDS), Fourier Transform Infrared spectroscopy with Attenuated Total Reflectance probe (FTIR-ATR), High Performance Liquid Chromatography with a Photodiode Array system (HPLC–PDA), X-ray Fluorescence spectroscopy (XRF), Zooarchaeology by Mass Spectrometry (ZooMS), and 14C dating were applied to an assemblage of textile and leather fragments that belong to the collection of the Byzantine & Christian Museum of Athens in Greece and lacked inventory information.The analyses applied, along with bibliographical research, yielded information on the materials, techniques and provenance of the find. The assemblage of finds consists of a gold thread embroidered silk satin, dyed with shellfish-purple; a gold strip-drawn wire embroidered silk tabby; a gold strip-drawn wire and red silk thread tapestry; a gold-thread embroidery on a tabby background; fragments of braided cord; and leather fragments. The material components could have been produced locally, in the area of the Byzantine Empire, though some of them indicate connections with India and/or China. The assemblage, dated between the mid AD 10th and mid eleventh century, was probably found in 1924 by Andreas Xygopoulos during the excavation of a tomb inside the church of Agia Sophia in Thessaloniki, Greece.
Intraoperative parathyroid hormone measurement pitfalls: parathyroid hormone spikes with carboxyl-terminal parathyroid hormone fragments in primary hyperparathyroidism—a case report
Authors: Yamashita Hiroyuki , Shindo Hisakazu , Yoshimoto Kouichi , Mori Yusuke , Fukuda Takashi , Tachibana Seigo , Takahashi Hiroshi , Sato Shinya 
Published: April 2024

Abstract
Background
Intraoperative parathyroid hormone (IOPTH) monitoring is a critical surgical adjunct for determining the extent of surgery for primary hyperparathyroidism (PHPT), with reported false-positive and false-negative rates of up to 10%. Surgeons must understand the parathyroid hormone (PTH) dynamics and select the appropriate IOPTH protocol and interpretation criteria for curative surgery.

Case presentation
We present the case of a 64-year-old woman with a large cystic parathyroid tumor and PHPT who experienced a significant delay in IOPTH decrease but was cured without additional surgery. The patient’s basal intact PTH was 96.2 pg/mL, which decreased to 93.3 pg/mL at 25 min and 72.4 pg/mL at 55 min after removal of the parathyroid tumor. In an attempt to elucidate its pathophysiology, 1–84 PTH levels were measured in stored serum. These results can also be attributed to the relatively low basal PTH levels, intact PTH spike, and high ratio of large carboxyl-terminal PTH fragments present. The patient had normal intact PTH and calcium levels at the 9-month postoperative visit.

Conclusions
As detailed reports on these phenomena are scarce, we discuss the causes of false-negative IOPTH results in terms of PTH production, secretion, metabolism, and differences in measurement methods to avoid unnecessary surgery.
A decision-making framework for the funding of shipping decarbonization initiatives in non-EU countries: insights from Türkiye
Authors: Baştuğ Sedat , Akgül Ersin Fırat , Haralambides Hercules , Notteboom Theo 
Published: April 2024

AbstractThe decarbonization of the shipping industry is a critical imperative in the global fight against climate change. Non-EU countries, being significant contributors to shipping emissions, play a crucial role in shaping the industry's sustainable future. However, securing funding for shipping decarbonization initiatives in these countries presents challenges, such as limited access to capital, lack of financial initiatives, political and regulatory uncertainties, technological risks, lack of local expertise, and the effects of global economic volatility. Addressing these challenges demands innovative strategies. The paper explores ways of effectively allocating funds for decarbonization projects in the shipping industry of non-EU countries, Türkiye in particular, using a structured decision-making framework tailored to their specific needs and challenges. We adopt the Moment Integrated Solution Method (THEMIS) to identify the “best” option from the range of alternative strategies identified in the framework. Our findings show that the use of incentive mechanisms is the most prioritized funding alternative, followed by the implementation of a sound Cap-and-Trade system and the promotion of strict local regulations to combat emissions. These imply that the prioritization of funding mechanisms, market-driven approaches, and more stringent regulation are key drivers in maritime decarbonization efforts. The Türkiye case study on strategies to achieve a greener maritime industry in developing countries also shows that securing funding for decarbonization requires a concerted effort by governments, private entities, and international organizations.
(No) Hope for the future? A design agenda for rewidening and rewilding higher education with utopian imagination
Authors: Nørgård Rikke Toft , Holflod Kim 
Published: April 2024

AbstractThis article argues for exploring, connecting, and applying utopian imagination, speculative design, and planetary thinking as a way forward for higher education to reimagine and move towards more hopeful planetary futures. It examines hopepunk and solarpunk perspectives on possible futures to propose a design agenda for rewidening and rewilding higher education and educational technology with utopian imagination. Firstly, the article outlines and develops a framework for wider and wilder futures in higher education, emerging from utopian thinking and desire. Secondly, it connects hopepunk with speculative design and solarpunk with planetary design to highlight and put forward rebellious strategies of hope in envisioning more preferable futures. Thirdly, it approaches the field of educational technology within the context of wide and wild education to establish four planetary orientations concerning educational technology: Higher Education for, in, with, and by the world. Taken together, the article proposes a design agenda for educational technology that integrates utopian imagination and solarpunk practices with planetary educational technology to catalyse the development of more preferable futures in a more-than-human world.
Pneumonic-type lung adenocarcinoma with KRAS G12V mutation and sustained response to Afatinib
Authors: Zhao Jie , Xu Jiachen , Qiu Tian , Wang Jie , Wang Zhijie 
Published: April 2024

Abstract
Background
Pneumonic-type lung adenocarcinoma (P-ADC) is a rare and challenging subtype of primary lung cancer that can be difficult to distinguish from pneumonia based on radiological images. Furthermore, no drugs are currently available that specifically target KRAS G12V.

Case presentation
Here we report a case of P-ADC with typical and informative imaging features throughout the course of the disease, including patchy shadows, high-density lesions with aerated bronchus, diffuse ground-glass opacities, and nodular shadows from computed tomography (CT) scan. The KRAS G12V mutation was detected using Next-generation sequencing (NGS). An individualized Afatinib-based therapeutic schedule was prescribed and achieved sustained response after multiple lines of treatment had failed.

Conclusion
Our case highlights the typical and dynamic changes in imaging features of P-ADC and provides an indicative treatment strategy for KRAS G12V-mutated lung adenocarcinoma.
Disruption of sleep by one night of in-home polysomnographic recording: a longitudinal actigraphy study of patients with chronic musculoskeletal pain and pain-free controls
Authors: Abeler Karin , Bergvik Svein , Friborg Oddgeir 
Published: April 2024

Abstract
Background
Patients with chronic pain frequently have comorbid sleep disturbances. Since improvement of sleep may alleviate both sleep problems and to some extent pain, sleep studies in this group becomes relevant. Polysomnography (PSG) is considered the gold standard for characterizing sleep; however, it is resource-demanding and may itself disrupt sleep due to the use of inconvenient equipment in unfamiliar sleep environments. To circumvent disruptive first night effect that may occur, sleep protocols may prescribe several nights of PSG to facilitate adaptation despite the equipment may still influence sleep on all nights. Moreover, the disruptive effects of polysomnography may vary between patient groups and healthy persons, yet have not previously been studied in patients with chronic pain. The present study aimed to assess whether sleep disruption during one night of in-home PSG was more severe in patients with chronic musculoskeletal pain compared to pain-free controls.

Method
Sleep was assessed by self-reported sleep quality and actigraphy measured sleep onset latency, sleep duration, wake after sleep onset and sleep efficiency during one night of in-home PSG and the following six nights in 56 patients and 53 pain-free participants. Additionally, sleep schedule was assessed by sleep onset time, wake up time and time in bed. The repeated sleep measures were analysed with mixed model regressions, comparing mean score changes between and within groups.

Results
A disruptive effect of PSG was evident for self-reported sleep quality and actigraphy measured sleep onset latency in both groups. These effects were however not significantly different between the groups, indicating comparable sensitivity to a single night of PSG between pain patients and pain-free controls.

Conclusion
These findings suggest that a singlenight in-home PSG protocol may be considered for case–control studies of patients with chronic pain.
Genetic diversity assessment of Trichoderma spp. isolated from various Egyptian locations using its gene sequencing marker, rep-PCR, and their cellulolytic activity
Authors: El-Sobky Muhammad Alaaeldin , Eissa Ragaa Abedlaziz , Abdel-Lateif Khalid Salah , Fahmi Abdelmegid Ibrahim , El-Zanaty Abdelfattah Mondy , Hassan Mohamed Mahmoud , Elsharkawy Mohsen Mohamed 
Published: April 2024

Abstract
Background
The phylogenetic relationships and phylogeny of twenty-six Trichoderma species collected from various Egyptian locations were investigated. The genetic diversity among the examined isolates was tested using the rep-PCR marker. Trichoderma species were screened for their cellulase activities.

Results
Three isolates demonstrated highly significant FPase activities, namely MNF-MAS-Tricho 1, MNF-MAS-Tricho 2, and MNF-MAS-Tricho 3 (0.50, 0.39, and 0.49 IU ml−1, respectively). MNF-MAS-Tricho1 showed the highest significant CMCase activity (0.80 IU ml−1). Concerning β-glucosidase, MNF-MAS-Tricho 1 was the highest (0.78 IU ml−1), while MNF-MSH-Trich 11 and MNF-MAS-Tricho 15 were the lowest (0.36 IU mL−1). The percentage of polymorphism ranged from 46.15 to 83.33%. (GTG)5 marker produced the greatest number of polymorphic loci (13 loci out of 18 loci) with about 83.33% polymorphism, followed by rep-10 with 69.2% polymorphism. Furthermore, the polymorphism information content (PIC) estimates ranged between 0.285 for Rep-10 and 0.340 for (GTG) 5 with an average of 0.306. The tested primers exhibited high discriminating and resolving powers.

Conclusion
The findings of this investigation were used to classify Trichoderma species, evaluate their genetic variability using ITS sequencing, rep-PCR, and measure their cellulase activities. These markers can facilitate more rapid and less complicated studies of Trichoderma population dynamics and evaluate their establishment after release into agricultural environments. The results will help to evaluate the genetic diversity of Trichoderma in future research.
Enhancing precision in vascular embolization: evaluating the effectiveness of the intentional early detachment technique with detachable coils in complex cases
Authors: Oka Shojiro , Kohno Shigeshi , Arizono Shigeki , Onishi Yasuyuki , Fumimoto Masaya , Yoshida Atsushi , Ishikura Reiichi , Ando Kumiko 
Published: April 2024

Abstract
Background
This study aimed to assess the effectiveness and versatility of an intentional early detachment technique with detachable coils in addressing challenging vascular embolization scenarios. This novel approach aims to provide an alternative method for achieving precise coil placement when standard methods of detachable coil placement are ineffective owing to vascular anatomy or limited available equipment.

Materials and methods
This retrospective study included 11 patients (nine males and two females; median age, 77 years) who underwent embolization procedures between October 2021 and December 2023 using the intentional early detachment technique through 1.6-Fr or 1.3-Fr microcatheters. In this technique, detachable coils were intentionally detached within the microcatheter and placed through saline flushing. The technique’s technical success, complications, and clinical success were evaluated.

Results
The technique was applied in three distinct scenarios: tortuous vascular anatomy (four cases), inadequate system backup (three cases), and 1.3-Fr microcatheter use (four cases). The technical and clinical success rates were 100%. No complications were observed, and no cases of coil migration or malpositioning.

Conclusion
The intentional early detachment technique is valuable for interventional radiologists and offers a solution for challenging vascular embolization scenarios. Its application is limited to specific circumstances; however, it can significantly enhance coil placement in complex cases, thereby contributing to improved patient care.

Graphical Abstract
Two putative calcium-dependent protein kinases are involved in the regulation of sugarcane defense genes
Authors: Li Xiangguo , Zhong Jiajie , Li Baiyang , Luo Yingfeng , Wang Kailun , Wang Yu , Ye Ziqin , Sun Lifan , Zhang Jinghan , Yang Liu , Wang Lixiang , Zhang Jie 
Published: April 2024

AbstractSugarcane is a primary sugar crop and an important source of bioenergy. Pathogens are the major factors affecting sugarcane yield and sugar content. However, the mechanisms of sugarcane defense regulation remain largely unknown, and research on prospective genetic targets for modification is scarce. As the main class of calcium sensors, calcium-dependent protein kinases (CDPKs/CPKs) play a crucial role in the immune regulatory network. Using sugarcane genomic data, we identified 229 putative ScCDPKs in primordial specie Saccharum officinarum. Searching the putative CDPKs in sugarcane cultivars from National Center for Biotechnology Information (NCBI), 12 putative ScCDPKs in cultivars were identified. Phylogenetic analysis revealed evolutionary relationships among these CDPKs in sugarcane cultivars and those in S. officinarum, Arabidopsis thaliana, and rice. Truncation mutants of ScCDPKs were introduced into the reporter system and examined for activity in inducing Sugarcane Pathogenesis Related Protein 1 (ScPR1). The results showed that truncated ScCDPK1 and ScCDPK8 induce higher expression of ScPR1 than full-length ScCDPK1 and ScCDPK8. Additionally, transient expression of truncated ScCDPK1 and ScCDPK8 exhibit stronger activity in sugarcane protoplasts for activation of ScPR1 and sugarcane Serine Protease Inhibitor (ScSPI). These results demonstrate that ScCDPK1 and ScCDPK8 possess auto-inhibitory activity. The findings of this study provide a basis for an in-depth study of the sugarcane CDPK gene family and lay the foundation for further genetic improvement.