bluuebunny/crossref_metadata_2025_split huggingface dataset:
Impacto de los escenarios potenciales de comportamientos disruptivos en la práctica médica: una revisión narrativa
Authors: Lora Villamil Nestor , Ariza Perez Diana , Brun Gudiño Ana , Moscote Salazar Luis , Garcia Ballestas Ezequiel 
Published: February 2025

La buena práctica médica es más que la correcta ejecución de procedimientos científicos, implica la adhesión de principios éticos y profesionales, garantizando la calidad de atención y ambiente laboral. Sin embargo, la falta de compromiso y cumplimiento de estos estándares generan una mala praxis que afecta tanto a los pacientes como al personal de la salud. Los comportamientos disruptivos se definen como interacciones verbales o físicas que afectan negativamente al ambiente laboral. Las principales conductas disruptivas en el ámbito médico incluyen el acoso, la intimidación y la agresividad, las cuales se asocian con consecuencias adversas como errores médicos, insatisfacción de los pacientes y un aumento en los costos de atención. A pesar de su impacto, estas conductas se han normalizado en muchos entornos médicos, convirtiéndose en un problema en aumento.

Factores como el estrés, la fatiga y las dificultades personales o laborales contribuyen significativamente a la aparición de comportamientos disruptivos. Por ello, resulta fundamental identificar y abordar estas conductas, promoviendo estrategias que fortalezcan las competencias del personal para resolver conflictos de manera efectiva. Además, los comportamientos disruptivos no solo deterioran la calidad del servicio, sino que también afectan la estabilidad mental y emocional de los profesionales. En consecuencia, resulta crucial implementar iniciativas que promuevan un ambiente colaborativo, respetuoso y libre de actitudes nocivas, con el objetivo de garantizar el bienestar del equipo médico y mejorar la atención brindada a los pacientes.
Design, synthesis, and in vitro, in vivo, and in silico evaluation of novel substituted 1,3,4-thiadiazole derivatives as anticonvulsant agents
Authors: Anthwal Tulika , Pant Swati , Rana Preeti , Nain Sumitra 
Published: February 2025

In the present study, a library of ten novel substituted 1,3,4‐thiadiazole derivatives were designed and synthesized using an appropriate synthetic route. The characterization of the synthesized compounds was performed by FT-IR and NMR (1H and 13C) spectroscopy. The synthesized compounds were assayed for in vitro human carbonic anhydrase (CA) inhibition against two isoforms II and IX. The neurotoxicity of the synthesized derivatives was also evaluated using the rotarod test, along with their in vivo anticonvulsant activity, which was determined using the maximal electroshock seizure (MES) and subcutaneous pentylenetetrazole (sc-PTZ) methods. Although all the compounds showed good CA inhibition and anticonvulsant activity, two compounds 6d and 7d showed the highest CA inhibition and anticonvulsant activity in both the isoforms and tested methods compared to the standard drugs (sodium valproate and acetazolamide), without any sign of neurotoxicity observed at the highest dose (300 mg/kg). Similarly, the standard drugs also displayed no neurotoxicity at the highest dose (300 mg/kg). Furthermore, the potent compounds (6d and 7d) were evaluated for the biochemical parameters, such as lipid peroxidation, nitrite oxide, reduced glutathione, superoxide dismutase, and total antioxidant capacity, and the GABA level was also determined. Finally, compound 6d was docked against CA-II and CA-IX (PDB-ID-5SZ5 and 5AML) receptors. The study concluded that the compounds 6d and 7d can be considered potent anticonvulsant agents for future research.
Simultaneous determination of 11 kinds of siloxanes in drinking water and source water using solid-phase microextraction combined with gas chromatography-mass spectrometry
Authors: Liu Sijie , Ma Jie , Jiang Su 
Published: February 2025

BackgroundSiloxanes have been widely used in various products and have been detected to varying degrees in the environment. It has been reported that methylsiloxane has toxic effects on the nervous, immune and reproductive systems of aquatic animals, and is carcinogenic and mutagenic. Therefore, in order to protect human health, it is urgent to establish a method for the determination of siloxane content in drinking water and source water.MethodsHerein, this paper proposes a precise, fast, and selective method using solid-phase microextraction combined with gas chromatography-mass spectrometry (GC-MS/MS) method for the simultaneous detection of 11 kinds of siloxanes in drinking water and source water. Quantification of siloxanes was carried out by internal standard method. The parameters that affect the extraction and desorption processes were optmised. The extraction efficiency of four commercially available SPME fibers was evaluated. The results showed that divinylbenzene/polydimethyl-siloxane or divinylbenzene/Carboxen/polydimethylsiloxane was the best coating for the extraction of siloxane.ResultsThis method provided good linearity (r &gt; 0.9946) and precision (RSD% &lt;8.0%) with the minimum detection mass concentration ranging from 0.008 to 0.025 μg/L under the optimized extraction and GC-MS/MS analysis conditions. The developed method has been applied to the simultaneous analysis of 11 kinds of siloxanes in drinking water and source water, and the results showed that decamethylcyclopentasiloxane (D5) and dodecamethylcyclohexasiloxane (D6) were found in two source water samples at concentrations ranging from 0.008 to 0.012 μg/L and 0.015–0.019 μg/L, respectively.ConclusionThis developed method was simple, quick, and effective and our satisfactory results demonstrated its suitability for the simultaneous determination of 11 kinds of siloxanes in drinking water and source water.
Body mass index impacts the lipid-lowing effects of statins in patients with type 2 diabetes
Authors: Sun Lulu , Wang Zhuo , Wang Bai , Jia Yuxiang , Zhao Qidi , Zhao Jingjun , Huang Xingtao 
Published: February 2025

BackgroundIndividuals diagnosed with type 2 diabetes mellitus (T2DM) commonly exhibit elevated lipid levels and an increased body mass index (BMI). The impact of BMI on the efficacy of statins in reducing lipid levels among diabetic patients remains uncertain. We aim to evaluate whether BMI will affect the lipid-lowing effects of statins in patients with T2DM.MethodsIn this retrospective analysis, we screened T2DM patients who were prescribed statins and underwent a 1-year outpatient follow-up recorded in the electronic medical record system. Patients were stratified into three groups: normal weight (BMI &lt; 24 kg/m), overweight (24 kg/m2 ≤ BMI &lt; 28 kg/m2), and obese (BMI ≥ 28 kg/m2). Lipid levels were assessed at two time points, and multivariate logistic regression analysis was used to identify factors influencing the reduction of low-density lipoprotein-cholesterol (LDL-C) levels and lipid control achievement.ResultsThis study included 289 patients, with 82 in normal weigh, 143 in overweight, and 64 in obese. Overweight and obese patients were found to be younger than those with normal weight. Over the 1-year follow-up period, lipid levels decreased in all patients, with a significant reduction observed in LDL-C levels. Notably, obese patients experienced the highest reduction in LDL-C levels compared to the normal and overweight groups (normal weight group ΔLDL 0.78 ± 0.95 mmol/L, p &lt; 0.001; overweight group ΔLDL 0.80 ± 0.88 mmol/L, p &lt; 0.001; obese group ΔLDL 1.11 ± 0.82 mmol/L, p &lt; 0.038). Obese patients exhibited a remarkable 42.02% reduction in LDL levels (normal 27.45%, overweight 30.64%). Multivariate logistic regression analysis indicated that achieving lipid control, defined as LDL &lt; 2.6 mmol/L, was more likely in obese patients compared to those with normal weight [odds ratio [OR] 3.48, 95% confidence interval [CI]: 1.18, 10.21, p = 0.023].ConclusionsThe effectiveness of statins in lowering lipid levels appears to be influenced by the patient's BMI in patients with T2DM. T2DM patients with high BMI may derive greater benefits, particularly in LDL reduction, from statin therapy.
Screen exposure, sleep quality, and language development in 6-month-old infants
Authors: Sanchez-Bravo Dayanara , Sandre Aislinn , Amarante Melina , Wiltshire Cynthia A. , Noble Kimberly G. 
Published: February 2025

IntroductionScreen time can have important ramifications for children's development and health. Children exposed to greater screen time score lower on assessments of language development and tend to sleep less. However, most studies examining associations among screen time, language development, and sleep quality have focused on older children and/or have relied on subjective assessments of screen time exposure (i.e., parent report). The current study examined whether screen exposure, assessed via both maternal-report questionnaires and in-home audio recordings, was associated with differences in language development and sleep quality in infants at ~6 months of age (N = 187).MethodsMothers completed questionnaires to assess infant screen exposure, language production, and sleep quality, as well as family socioeconomic and demographic factors. The Language Environment Analysis (LENA) recorder was used to measure home screen use and the language environment.ResultsHigher family income and higher maternal education were associated with less infant screen time, as assessed by both maternal report and in-home LENA recordings. Neither measure of infant screen exposure was significantly associated with the home language environment, maternally-reported infant language production, or infant sleep quality. Maternally-reported screen exposure showed a small but significant positive correlation with LENA-derived screen exposure.DiscussionWe find no detectable association between screen exposure and differences in maternally reported language development or sleep quality in the first 6 months of life. Future studies will be needed to examine associations among screen time and subsequent infant development and health outcomes.
Mosquito fauna (Diptera: Culicidae) in phytotelmata environments of the Guapiaçu Ecological Reserve, Cachoeiras de Macacu, Rio de Janeiro, Brazil
Authors: de Almeida Nathália Menezes , Dias Rayane Ferreira , Alves Dálete Cássia Vieira , Machado Sergio Lisboa , Silva Júlia dos Santos , de Mello Cecilia Ferreira , Alencar Jeronimo 
Published: February 2025

IntroductionPhytotelmata are plant structures that collect and retain water, serving as natural larval habitats. Certain species of culicids utilize these water-holding structures for larval development, playing a crucial role in water retention and nutriente cycling, which benefits resident fauna in tropical forests, such as the Atlantic Forest. This study aimed to characterize the taxonomic composition, diversity, species richness, and abundance of mosquitoes within three types of phytotelmata in an Atlantic Forest remnant.MethodsMonthly monitoring of mosquito populations was conducted over one year, from March 2023 to February 2024, considering abiotic factors such as temperature, pH, and rainfall, which can influence mosquito larval growth and abundance. Sampling occurred in selected phytotelm habitats along trails within the study area.ResultsA total of 2,672 culicid specimens were collected, representing 11 genera and 29 species. The most abundant species were Wyeomyia artrostigma, Culex pleuristriatus, and Onirion personatum. Bamboo internodes yielded the highest abundance of specimens (67.4%), followed by bromeliads (30.7%) and buttress roots (1.9%). This distribution pattern may be due to the varying suitability of diferente phytotelmata as larval habitats, with bamboo internodes providing more favorable environmental conditions for larval survival. Among types of phytotelmata, bamboo internodes had the highest species richness (22 species) and the largest total number of specimens, highlighting this habitat’s importance for the development of many mosquito species. In contrast, buttress roots, despite having fewer specimens, showed the greatest diversity and evenness, indicating a balanced species distribution within this habitat. Kruskal-Wallis analysis revealed significant differences in species abundance among the types of phytotelmata evaluated, suggesting that mosquito population composition and abundance are habitat-dependent.DiscussionThese results support the idea that different phytotelmata offer distinct ecological conditions, influencing the occurrence and development of culicid populations. Notable among the species collected were Aedes albopictus and Haemagogus leucocelaenus, known vectors of human pathogens. This study provides valuable insights into the mosquito fauna associated with phytotelmata in an Atlantic Forest remnant, contributing to our understanding of the diversity and ecological dynamics of these environments. Our findings also have public health implications, demonstrating the presence of vector species in a natural habitat, which could inform future research and disease control strategies.
CD21low B cells reveal a unique glycosylation pattern with hypersialylation and hyperfucosylation
Authors: Felixberger Peter Tobias , Andrieux Geoffroy , Maul-Pavicic Andrea , Goldacker Sigune , Harder Ina , Gutenberger Sylvia , Landry Jonathan J. M. , Benes Vladimir , Jakob Till Fabian , Boerries Melanie , Nitschke Lars , Voll Reinhard Edmund , Warnatz Klaus , Keller Baerbel 
Published: February 2025

BackgroundThe posttranslational modification of cellular macromolecules by glycosylation is considered to contribute to disease pathogenesis in autoimmune and inflammatory conditions. In a subgroup of patients with common variable immunodeficiency (CVID), the occurrence of such complications is associated with an expansion of naïve-like CD21low B cells during a chronic type 1 immune activation. The glycosylation pattern of B cells in CVID patients has not been addressed to date.ObjectiveThe objective of this study was to examine the surface glycome of B cells in patients with CVID and associated immune dysregulation.MethodsWe performed surface lectin staining on B cells from peripheral blood and tonsils, both ex vivo and after in vitro stimulation. Additionally, we examined the expression of glycosylation-related genes by RNAseq in naïve-like CD21low B cells ex vivo, as well as in naïve CD21pos B cells from healthy controls after in vitro stimulation.ResultsUnlike CD21pos B cells, naïve-like CD21low B cells from CVID patients and CD21low B cells from healthy controls exhibited a unique glycosylation pattern with high levels of α2,6 sialic acids and fucose. This hypersialylation and hyperfucosylation were particularly induced by activation with anti-IgM and interferon-γ (IFN-γ). Transcriptome analysis suggested that naïve-like CD21low B cells possess a comprehensively reorganised glycosylation machinery, with anti-IgM/IFN-γ having the potential to initiate these changes in vitro.ConclusionCD21low B cells are hypersialylated and hyperfucosylated. This may implicate altered lectin-ligand interactions on the cell surface potentially affecting the CD21low B-cell function. These glycome changes appear to be driven by the prominent type I immune response in complicated CVID patients. A better understanding of how altered glycosylation influences immune cell function could lead to new therapeutic strategies.
Case report: Patients with positive HER-2 amplification locally advanced gastroesophageal junction cancer achieved pathologic complete response with the addition of pembrolizumab to chemotherapy plus trastuzumab as neoadjuvant therapy
Authors: Li Huanhuan , Ren Chao , Cui Donghai , Wu Tao , Nie Zhiyong 
Published: February 2025

BackgroundHuman epidermal growth factor receptor 2(HER-2) is the most prominent therapeutic target for gastric (G)/gastroesophageal junction (GEJ) cancer. Guidelines recommend its use for treating G/GEJ cancers. However, targeted therapy did not significantly improve survival outcomes compared to those with neoadjuvant therapy. The KEYNOTE-811 trial revealed an improved objective response rate (74% vs. 52%; P=.0001) and median duration of response (10.6 vs 9.5 months) with the addition of pembrolizumab (PD-1) to chemotherapy plus trastuzumab compared to that with the addition of placebo in patients with HER-2 overexpression-positive advanced adenocarcinoma. Therefore, addition of PD-1 to chemotherapy plus trastuzumab may lead to a better response in patients with G/GEJ cancer.Case presentationA 66-year-old man was diagnosed with stage III GEJ adenocarcinoma with celiac lymph node metastasis. Immunohistochemical results indicated HER-2(3+) and PD-L1 CPS=5. The patient received three cycles of pembrolizumab plus trastuzumab and chemotherapy preoperatively and underwent radical surgery on November 22, 2022.ConclusionPatients with HER-2-positive locally advanced GEJ cancers received PD-1 immunotherapy combined with trastuzumab and neoadjuvant chemotherapy and achieved a complete pathological response. Hence, it is a novel, highly specific, and potent therapeutic option for HER-2-positive patients and should henceforth be considered as a new treatment approach.
Intelligent diagnosis and prediction of pregnancy induced hypertension in obstetrics and gynecology teaching by integrating GA
Authors: Li Xiaolan , Kang Fen , Li Xiaojing 
Published: February 2025

TitleAdvanced Diagnosis and Forecasting of Pregnancy-Induced Hypertension in Obstetrics and Gynecology Education through the Integration of Genetic Algorithms.BackgroundPregnancy-induced hypertension represents a critical issue within the fields of obstetrics and gynecology, where precise diagnosis and forecasting are essential for effective management. The potential for misdiagnosis, often stemming from the inexperience of healthcare professionals, underscores the necessity for an advanced diagnostic system.MethodsThis research introduces an innovative sampling and feature selection technique grounded in F-scores optimization, alongside the development of a comprehensive prediction model that integrates genetic algorithms with various heterogeneous learners. The objective of this model is to maximize the utility of medical data and enhance treatment quality.ResultsThe refined intelligent feature selection approach identified several significant indicators of pregnancy-related hypertension, such as phosphor dehydrogenase deficiency, body mass index, gestational urinary proteins, vascular endothelial growth factor receptor 1, placental growth factor, thalassemia, and a familial history of diabetes mellitus or hypertension. The model achieved superior performance metrics, including the highest recall (0.768), F-score (0.728), and area under the curve (0.832) when compared to other prevalent models. Furthermore, the area under the curve for both early and late clinical assessments reached peak values of 0.996 and 0.792, respectively, when evaluated using the ratio of vascular endothelial growth factor receptor 1 to placental growth factor.ConclusionThe intelligent diagnosis and prediction methodology for gestational hypertension proposed in this study exhibited remarkable efficacy and holds significant promise for implementation in both educational and clinical settings within obstetrics and gynecology, thereby advancing intelligent medical diagnostics in China.
Case report: Clinical characteristics of anthrax meningoencephalitis: two cases diagnosed using metagenomic next-generation sequencing and literature review
Authors: Hu Minzhe , Qiao Xiaodong , Zhang Jingliang , Qin Danqing , Guo Shougang , Zhao Weili , Wang Chunjuan 
Published: February 2025

PurposeTo explore the clinical features, diagnosis, treatment, and prognosis of anthrax meningoencephalitis.MethodsThe clinical data of two cases of anthrax meningoencephalitis were summarized and the relevant literature was reviewed.ResultsBoth patients, who were farmers, had cutaneous lesions prior to the onset of meningoencephalitis. The clinical manifestations included fever (2/2), headache (2/2), stupor (2/2), meningeal signs (2/2), and lymph node enlargement (2/2). The CSF analysis showed erythrocytes, increased neutrophils, low glucose levels and high protein levels. CSF cytology revealed rod-shaped bacilli. Metagenomic next-generation sequencing of the CSF from both patients detected Bacillus anthracis. Additionally, cultures confirmed the presence of endogenous spores of macrobacteria. Brain imaging revealed subarachnoid hemorrhages and minimal cerebral edema. Despite aggressive antibiotic treatment, both patients died. Fifty-seven articles of the past 70 years were reviewed. There were 59 patients of anthrax meningoencephalitis in total, including 46 patients died. Stupor (42/46, 91.3% vs. 3/13, 46.2%, p = 0.001), agitation (15/46, 32.6% vs. 0/13, 0.0%, p = 0.043) and intracranial hemorrhage (37/46, 80.4% vs. 4/13, 30.8%, p = 0.002) were more common in the deceased group. Two types of bactericidal drugs or intrathecal injection drugs presented more often in the surviving group (10/13, 76.9% vs. 13/46, 28.3%, p = 0.001), whereas penicillin monotherapy presented more often in the deceased group (23/46, 50.0% vs. 2/13, 15.4%, p = 0.026).ConclusionAnthrax meningoencephalitis typically presents as a rapidly progressive bacterial meningoencephalitis. The occurrence of stupor, agitation and intracranial hemorrhage is possibly correlated with poor outcome. Two types of bactericidal drugs or intrathecal injection drugs are associated with better prognosis. Metagenomic next-generation sequencing can quickly and accurately detect B. anthracis in CSF.
Two sexually compatible monokaryons from a heterokaryotic Lentinula edodes strain respond differently to heat stress
Authors: Guo Yuan , Jiao Wenyu , Zhang Yajie , Tan Meiting , Gao Qi , Liu Yu , Wang Shouxian 
Published: February 2025

BackgroundDespite the extensive research conducted on heat responses of Lentinula edodes heterokaryotic cells, the responses of the two sexually compatible monokaryons to heat stress (HS) remain largely unknown.MethodsTo bridge this gap, we examined the nucleus-specific (SP3 and SP30) heat resistant mechanisms using an integrated physiological, metabolomic and transcriptomic approach.ResultsThe results showed that HS elicited the boost of ROS and hampered mycelium growth for both monokaryons. Metabolome and transcriptome analysis demonstrated that the two sexually compatible monokaryons responded differently to HS. For SP3, the differentially expressed genes (DEGs) were significantly enriched in Mitogen-Activated Protein Kinase (MAPK) signaling, cell cycle and sugar metabolism, whereas those DEGs for SP30 were enriched in glyoxylate and dicarboxylate metabolism, and protein processing. The differentially accumulated metabolites (DAMs) of both strains were enriched in the glycerophospholipid metabolism, alpha-linolenic acid metabolism, biosynthesis of cofactors, etc, but were regulated differently in each strain. The enriched KEGG pathways for SP3 tend to be downregulated, whereas those in SP30 exhibited a contrary trend. The genes in MAPK signaling pathway were associated with the glycerophospholipid metabolism in SP3, but not in SP30. Omics-integration analysis revealed distinguishing regulatory networks and identified completely different hub genes for the two strains.DiscussionOur findings revealed, for the first time, the different heat-resistance mechanisms of the two compatible nuclei and provided candidate metabolites, responsive genes and regulatory pathways for further experimental validation.