bluuebunny/crossref_metadata_2025_split huggingface dataset:
Binding Studies and Effect of Light on the Conductance of Intercalated Curcumin into DNA
Authors: Subhan M. A. , Islam M. M. , Chowdhury M. R. U. 
Published: May 2012

Curcumin is one of the most valuable ingredients for scientific research in view of medicinal and therapeutic importance. Curcumin is reported to have antitumor and anticancer activities. It can easily be extracted from Turmeric by solvent extraction methods. At first, dye was extracted through reflux and trituration process. Then curcumin was isolated from dye using column chromatography, which was investigated by infrared spectroscopy and melting point. Curcumin formed a complex with Ce3+, Ce(C21H20O6)n, which was confirmed by comparing the IR spectrum of complex with that of ligand. We observed the effect of light on the curcumin intercalated into DNA and found that the electrons in curcumin excited by absorption of light and the conductance of the solution increased gradually when irradiated by UV-light. The result may indicate the electron conducting property of DNA in solution. We focused on the binding of curcumin with DNA. Our investigations also showed that both curcumin and Ce3+ complex have luminescence properties upon irradiation by UV-light.Keywords: Curcumin; Ce3+-Complex; Luminescence; DNA; Antioxidant.© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v4i2.8482 J. Sci. Res. 4 (2), 411-418 (2012)
Physico-Chemical Characterization and Biological Screening of Cyanex 302 complexes
Authors: Pal T. K. , Alam M. A. , Islam M. A. A. A. A. , Paul S. R. 
Published: May 2012

Several new complexes of Cr(III), Mn(II), Fe(II), Co(II) and Cu(II) containing Cyanex 302 [bis(2,4,4-trimethylpentyl) monothiophosphinic acid] were synthesized and characterized by elemental analysis, molar conductance, molecular mass determination, magnetic, UV-Visible and IR studies. The prepared metal complexes has the compositions: 2. [CrL3].H2O, 3. K[MnL3].H2O, 4. [FeL2].H2O, 5. K[CoL3] and 6. K[CuL3].H2O (Where, L means anion of bis(2,4,4-trimethylpentyl) monothiophosphinic acid). The complexes 2, 3, 5 and 6 are assumed to have octahedral geometries, but the complex 4 is tetrahedral based on experimental data. From magnetic measurements all the complexes are found to be paramagnetic. Measured molar conductance showed that the complexes 2 and 4 are non-electrolytes and complexes 3, 5 and 6 are electrolytes. Besides, some metal complexes have shown very good antibacterial and antifungal activities.Keywords: Bis(2,4,4-trimethylpentyl) monothiophosphinic acid; Biological activity.© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v4i2.9366 J. Sci. Res. 4 (2), 427-435 (2012)
Numerical Solutions of Volterra Integral Equations Using Laguerre Polynomials
Authors: Rahman M. A. , Islam M. S. , Alam M. M. 
Published: May 2012

We solve numerically Volterra integral equations, of first and second kind with regular and singular kernels, by the well known Galerkin weighted residual method. For this, we derive a simple and efficient matrix formulation using Laguerre polynomials as trial functions. Several numerical examples are tested. The approximate solutions of some examples coincide with the exact solutions on using a very few Laguerre polynomials. The approximate results, obtained by the present method, confirm the convergence of numerical solutions and are compared with the existing methods available in the literature.Keywords: Volterra integral equations; Galerkin method; Laguerre polynomials.© 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved.doi: http://dx.doi.org/10.3329/jsr.v4i2.9407 J. Sci. Res. 4 (2), 357-364 (2012)
Incidence of Human Skin Pathogens from Cosmetic Tools used in Beauty Saloons in Different Areas of Lahore, Pakistan
Authors: Naz S. , Iqtedar M. , Ain Q. ul , Aftab K. 
Published: May 2012

With the surprising development across the cosmetic and personal care companies the re-utilization of cosmetic tools is of a common practice. Isolation and detection of human skin pathogens from 100 samples of beauty salon tools i.e., blusher brush, face sponge and wax has been done. All the samples were examined microbiologically for the contamination of Staphylococcus aureus, Pseudomonas aeruginosa, yeast and fungus. It was observed that the percentage of Staphylococcus aureus was higher (100% in sponge, 100% in brush, 88% in wax) in the tools than Pseudomonas aeruginosa (69.6% in sponge, 81.8% in brush and 73.5% in wax), where counts obtained for fungus was 51.5% in sponge, 30.3% in brush and 20.5% in wax. It was observed that the major cause of contamination of saloon tools is repetitive usage on all costumers without considering the hygienic conditions. Keywords: Human skin pathogens; Cosmetic contamination; Pseudomonas in cosmetics; S.aureus in cosmetics. © 2012 JSR Publications. ISSN: 2070-0237 (Print); 2070-0245 (Online). All rights reserved. doi: http://dx.doi.org/10.3329/jsr.v4i2.9640 J. Sci. Res. 4 (2), 523-527 (2012)
Density of Microbial Colonization on External and Internal Surfaces of Concurrently Placed Intravascular Devices
Authors: Koh David Boon Chai , Robertson Iain Kilpatrick , Watts Marianne , Davies Andrew Neil 
Published: May 2012

Background
Intravascular devices provide essential vascular access for management of critically ill patients but can be associated with bloodstream infections.

Objectives
To determine colonization rates in segments of concurrently placed peripheral arterial, nontunnelled short-term central venous catheters and peripherally inserted central catheters and the pattern of heaviest colonization when the catheters are removed.

Methods
A prospective study was conducted on inpatients with intravascular devices in place for 9 days or more. At removal, each catheter was cut into 3 segments, and each segment was cultured separately. The density of colonization on external and internal surfaces of each segment was compared by estimating odds ratios by repeated-measures ordinal logistic regression.

Results
A total of 48 peripheral arterial, 135 central venous, and 106 peripherally inserted central catheters were obtained from 289 patients. Colonization was greatest at the proximal external segment of all catheters. On the external surface, colonization was lower on the middle (odds ratio, 0.70; P &lt; .001) and distal (odds ratio, 0.56; P &lt; .001) segments than on the proximal segments. On the internal surface, colonization was lower on the proximal (odds ratio, 0.39; P &lt; .001), middle (odds ratio, 0.30; P &lt; .001), and distal (odds ratio, 0.31; P &lt; .001) segments than on the external proximal segments. This trend was similar for all catheter types.

Conclusion
Colonization of intravascular devices was heaviest on proximal segments.
Projection of FMRFamide-like neuropeptide-producing neurosecretory cells from silkworm brain into ventral nerve cord and retrocerebral complex
Authors: Kim Bo Yong , Song Hwa Young , Kim Mi Young , Kang Pil Don , Cha Min Ho , Park Hun Hee , Lee Seung Gwan , Lee Chang Kyou , Yu Chai Hyeock , Lee Bong Hee 
Published: May 2012

AbstractUsing immunostaining methodology, we traced the axonal projection of FMRFamide (Phe-Met-Arg-Phe-NH2)-like immunoreactive (LI) medial neurosecretory cells (MNCs) and lateral neurosecretory cells (LNCs) from the brain into the ventral nerve cord (VNC) and retrocerebral complex inBombyx mori(L.) (Lepidoptera: Bombycidae). Of the seven pairs of FMRFamide-LI MNCs, one pair extended its axons from the brain pars intercerebralis into the VNC ipsilateral connective where they appeared to terminate. The axons of the remaining MNCs ran through decussation in the brain median region and contralateral nervi corporis cardiaci (NCC) I out of the brain, and eventually innervated the contralateral corpus cardiacum (CC). Axons from the single pair of FMRFamide-LI LNCs projected into the ipsilateral NCC II fused with NCC I without decussation in the brain, and finally terminated in the CC. These results suggest that transport of the FMRFamide-like neuropeptide from may be related to the modulation of functions such as gut contraction in MNCs terminating in the VNC, and regulation of production and/or secretion of specific hormones such as juvenile hormone in MNCs and LNCs terminating in the CC.
C. P. Alexander review
Authors: Rhainds Marc , Kettela Edward G. , Silk Peter J. 
Published: May 2012

AbstractThe Canadian registration in 2007 of Disrupt SBW Micro-Flakes®, a pheromone-based product for control of spruce budworm,Choristoneura fumiferana(Clemens), paved the way for large-scale trials to test the practicality of mating disruption as a commercial pest management strategy. We review results from field and laboratory experiments on pheromone-based mating disruption of spruce budworm conducted from 1974 to 2008. Application of pheromone from the ground or the air consistently reduced the orientation of males toward pheromone sources. Mating disruption also reduced the mating success of caged or tethered females in 15 of 16 field studies where this parameter was recorded, but had only a limited effect on the mating success of feral females. No consistent difference in the density of egg masses in control and treated plots was observed, which has often been attributed to immigration of gravid females into pheromone-treated plots. Laboratory studies suggest that false-trail following is the predominant mechanism underlying mating disruption in spruce budworm. The enhanced mating success of females with increasing population density suggests that mating disruption should target low-density emergent populations during the initial phase of an outbreak. Constraints that may limit the potential of mating disruption as a management tool include (1) difficulties associated with obtaining accurate sampling estimates at low population density to forecast the onset of outbreaks, (2) potential behavioral adaptations by which females enhance their mating success when the atmosphere is treated with pheromone, and (3) long-range dispersal of females by flight.
Protective Role of Reactive Oxygen Species in Endotoxin-Induced Lung Inflammation through Modulation of IL-10 Expression
Authors: Deng Jing , Wang Xuerong , Qian Feng , Vogel Stephen , Xiao Lei , Ranjan Ravi , Park Hyesuk , Karpurapu Manjula , Ye Richard D. , Park Gye Young , Christman John W. 
Published: May 2012

Abstract
Reactive oxygen species (ROS) generated by NADPH oxidase are generally known to be proinflammatory, and it seems to be counterintuitive that ROS play a critical role in regulating the resolution of the inflammatory response. However, we observed that deficiency of the p47phox component of NADPH oxidase in macrophages was associated with a paradoxical accentuation of inflammation in a whole animal model of noninfectious sepsis induced by endotoxin. We have confirmed this observation by interrogating four separate in vivo models that use complementary methodology including the use of p47phox−/− mice, p47phox−/− bone marrow chimera mice, adoptive transfer of macrophages from p47phox−/− mice, and an isolated perfused lung edema model that all point to a relationship between excessive acute inflammation and p47phox deficiency in macrophages. Mechanistic data indicate that ROS deficiency in both cells and mice results in decreased production of IL-10 in response to treatment with LPS, at least in part, through attenuation of the Akt-GSK3-β signal pathway and that it can be reversed by the administration of rIL-10. Our data support the innovative concept that generation of ROS is essential for counterregulation of acute lung inflammation.
Delays and Diversions Mark the Development of B Cell Responses to Borrelia burgdorferi Infection
Authors: Hastey Christine J. , Elsner Rebecca A. , Barthold Stephen W. , Baumgarth Nicole 
Published: May 2012

Abstract
B cell responses modulate disease during infection with Borrelia burgdorferi, the causative agent of Lyme disease, but are unable to clear the infection. Previous studies have demonstrated that B. burgdorferi infection induces predominantly T-independent B cell responses, potentially explaining some of these findings. However, others have shown effects of T cells on the isotype profile and the magnitude of the B. burgdorferi-specific Abs. This study aimed to further investigate the humoral response to B. burgdorferi and its degree of T cell dependence, with the ultimate goal of elucidating the mechanisms underlying the failure of effective immunity to this emerging infectious disease agent. Our study identifies distinct stages in the B cell response using a mouse model, all marked by the generation of unusually strong and persistent T-dependent and T-independent IgM Abs. The initial phase is dominated by a strong T-independent accumulation of B cells in lymph nodes and the induction of specific Abs in the absence of germinal centers. A second phase begins around week 2.5 to 3, in which relatively short-lived germinal centers develop in lymph nodes, despite a lymph node architecture that lacks clearly demarcated T and B cell zones. This response failed, however, to generate appreciable numbers of long-lived bone marrow plasma cells. Finally, there is a slow accumulation of long-lived Ab-secreting plasma cells in bone marrow, reflected by a strong but ultimately ineffective serum Ab response. Overall, the study indicates that B. burgdorferi might evade B cell immunity by interfering with its response kinetics and quality.
Crystal Structure of Cell Adhesion Molecule Nectin-2/CD112 and Its Binding to Immune Receptor DNAM-1/CD226
Authors: Liu Jun , Qian Xiaomin , Chen Zhujun , Xu Xiang , Gao Feng , Zhang Shuijun , Zhang Rongguang , Qi Jianxun , Gao George F. , Yan Jinghua 
Published: May 2012

Abstract
The nectin and nectin-like molecule (Necl) family includes important cell adhesion molecules (CAMs) characterized by their Ig-like nature. Such CAMs regulate a broad spectrum of cell–cell interactions, including the interaction between NK cells and cytotoxic T lymphocytes (CTLs) and their target cells. CAM members nectin-2 (CD112) and Necl-5 (CD155) are believed to form homodimers (for nectin-2) or heterodimers in their functions for cell adhesion, as well as to interact with immune costimulatory receptor DNAX accessory molecule 1 (DNAM-1) (CD226) to regulate functions of both NK and CTL cells. However, the structural basis of the interactive mode of DNAM-1 with nectin-2 or Necl-5 is not yet understood. In this study, a soluble nectin-2 Ig-like V-set domain (nectin-2v) was successfully prepared and demonstrated to bind to both soluble ectodomain and cell surface-expressed full-length DNAM-1. The 1.85-Å crystal structure of nectin-2v displays a perpendicular homodimer arrangement, revealing the homodimer characteristics of the nectin and Necls. Further mutational analysis indicated that disruption of the homodimeric interface of nectin-2v led to a failure of the homodimer formation, as confirmed by crystal structure and biochemical properties of the mutant protein of nectin-2v. Interestingly, the monomer mutant also loses DNAM-1 binding, as evidenced by cell staining with tetramers and surface plasmon resonance assays. The data indicate that interaction with DNAM-1 requires either the homodimerization or engagement of the homodimeric interface of nectin-2v. These results have implications for immune intervention of tumors or autoimmune diseases in the DNAM-1/nectin-2–dependent pathway.
Correlation between Dental Traumatic Injuries and Overjet among 11 to 17 years Indian Girls with Angle's Class I Molar Relation
Authors: Bendgude Vikas , Akkareddy Basavaprabhu , Panse Amey , Metha Deepak , Jawale Bhushan , Garcha Vikram , Jathar Prasad 
Published: May 2012

ABSTRACTAimThe present study was conducted to assess the correlation of traumatic dental injuries and overjet among school going girls of age 11 to 17 years in Pune, India.Materials and methodsA cross-sectional survey was carried out with 2045 school girls aged 11 to 17 years, attending public elementary schools in Pune, India. The traumatic dental injuries were assessed according to Ellis and Davey's criteria. Occlusion was recorded according to Angle's classification and overjet was measured using a WHO 621 probe. The data obtained was subjected to statistical analysis using Mann-Whitney test.ResultsThere was a significant (Z −2.785) association between increase in overjet and occurrence of trauma. Children with increased overjet were 3.09 times more likely to have dental injuries.ConclusionIt can be concluded from the present study that a significant correlation exists between amount of overjet and prevalence of traumatic injuries among children. The percentage of trauma prevalence increases with the increase of overjet.Clinical significanceTraumatic dental injury is not a result of disease but a consequence of several factors that will accumulate throughout life if not properly treated. One of the most important factors significantly contributing to traumatic dental injuries is the anterior overjet. Hence, correction of increased anterior overjet may help in prevention of traumatic injuries.How to cite this articleBendgude V, Akkareddy B, Panse A, Singh R, Metha D, Jawale B, Garcha V, Jathar P. Correlation between Dental Traumatic Injuries and Overjet among 11 to 17 years Indian Girls with Angle's Class I Molar Relation. J Contemp Dent Pract 2012;13(2):142-146.
Effect of Immediate and Delayed Postpreparation on the Integrity of the Apical Seal: An in vitro Study
Authors: Kamra Abhay , Choudhari Shantanu , Bhondwe Sarvesha , Srilatha S , Desai Neha Chhasatia , Pandit Varsha Sanjay 
Published: May 2012

ABSTRACT

Aim
The aim of this in vitro study was to evaluate if there is any significant difference in apical leakage when gutta-percha is removed immediately after obturation for postspace preparation or after a week.

Materials and methods
Two commonly used sealers AH26 and tubliseal were used in four groups each consisting of 20 teeth each. The tooth was sectioned at the amelocemental junction to leave a root portion of 12 to 14 mm. Canals were checked for patency and prepared to No-55 K file size. Two people, using a stereomicroscope, independently evaluated each tooth-half for the extent of apical leakage.

Results
The leakage results were analyzed using a one-way ANOVA according to present study, immediate postpreparation is preferable than delayed postpreparation. The relationship of in vitro leakage measurements to the in vivo situation has not been established.

Clinical implication
Hence, immediate postpreparation is preferable than delayed postpreparation.

How to cite this article
Bhondwe S, Kamra A, Choudhari S, Srilatha S, Desai NC, Pandit VS. Effect of Immediate and Delayed Postpreparation on the Integrity of the Apical Seal: An in vitro Study. J Contemp Dent Pract 2012;13(2):153-156.
Expression of Extracellular Matrix—Laminin in Oral Squamous Cell Carcinoma: An Immunohistochemical Study
Authors: Kapse Sonam C , Koshy Ajit V , Rao Nirmala N , Kamat Sushant S , Kiswani Kamal , Shaikh Nahid A 
Published: May 2012

ABSTRACT

Aim
To evaluate the expression of laminin in various grades of oral squamous cell carcinoma (OSCC) in order to determine whether this protein can be used as a marker for early detection and elucidation of oral cancer.

Materials and methods
Immunohistochemical staining for laminin was done on 60 selected archival blocks of histopathologically diagnosed cases of primary OSCC and the laminin expression was compared between the different histopathological grades of primary OSCC. The statistical analysis was performed by using Chi-square (÷ square) test and Gaussiantest with a probability of p < 0.05 was considered as significant.

Results
It was observed that laminin expression decreased with tumor progression which may be correlated to the tumor aggressiveness.

Conclusion
There was a gradual decrease of laminin staining with decreasing cellular differentiation, with differentiated lesions showing a more conspicuous staining of basement membrane glycoprotein than less differentiated lesions.

Clinical significance
An understanding of how the extracellular matrix influences tumor development and invasion is fundamental in the development of new prognostic indicators and treatment strategies for oral squamous cell carcinoma.

How to cite this article
Koshy AV, Rao NN, Kamat SS, Kiswani K, Kapse SC, Shaikh NA. Expression of Extracellular Matrix— Laminin in Oral Squamous Cell Carcinoma: An Immunohistochemical Study. J Contemp Dent Pract 2012;13(2):194-200.
Saliva: A Powerful Diagnostic Tool for Minimal Intervention Dentistry
Authors: Ranganath LM , Rajesh AG , Shet RGK 
Published: May 2012

ABSTRACT
Saliva plays a vital role in oral health as patients strive to maintain a healthy dentition throughout their lives. It is nature's primary defense mechanism for the oral environment, and is particularly important for protecting exposed tooth surfaces. While internal protection for dentin comes from odontoblasts and the dental pulp, the body's external protection for enamel comes from saliva.
The noninvasive nature of salivary testing has made it an effective alternative to blood and urine testing and home testing kits have made it possible for people to monitor their own health using this diagnostic medium. This paper presents what saliva can reveal about general and oral health as well as highlights the current use and potential clinical and research applications, of diagnostics based on oral fluids.

Clinical significance
Early detection always minimizes the need for more invasive treatment. It prevents oral health disease at an early stage and provides a good oral health in rejuvenated state. If you stick and follow regular professional care, prevention maintenance appointments, prevention counseling, good home care and oral hygiene, diet habits you will be free from oral health illness and you can experience the harmonious and rejuvenated state of good oral health.

How to cite this article
Ranganath LM, Shet RGK, Rajesh AG. Saliva: A Powerful Diagnostic Tool for Minimal Intervention Dentistry. J Contemp Dent Pract 2012;13(2):240-245.
Behavior Shaping of Complete Denture Patient: A Theoretical Approach
Authors: Sudheer A , Reddy G Vivekananda , Reddy Giridhar 
Published: May 2012

ABSTRACT
The psychological status of a geriatric patient undergoing complete denture treatment is one of the key determinants of the success of prosthesis. Hence, the understanding and behavior management of such patients is of paramount importance for any clinician aspiring to be a successful in practice. Even though several attempts have been made to understand the effects of psychology of patients undergoing this particular treatment on the final outcome of treatment, no single theory or classification has been proposed so far as to be able to completely understand the thought process of geriatric patients. The aim of this paper is to propose a theoretical approach, a step by step guide to clinicians to better understand the thoughts, aspirations and expectations of complete denture patients and their effects and consequences in different patients, when not met with.

How to cite this article
Sudheer A, Reddy GV, Reddy G. Behavior Shaping of Complete Denture Patient: A Theoretical Approach. J Contemp Dent Pract 2012;13(2):246-250.