bluuebunny/crossref_metadata_2025_split huggingface dataset:
Pad Printing of Carbon Electrodes with Argon Plasma Activation as a Simple and Low Temperature Manufacturing Process for Antibody‐Type Biosensors
Authors: Condemi Enrico , Kunikowski Joanna , Schoinas Spyridon , Passeraub Philippe 
Published: May 2024

AbstractIn diagnostic tools, rapid in vitro tests such as COVID‐19 antigen or pregnancy tests are gaining significance for identifying various pathologies or health conditions. This shift contributes to a change in the way diagnostic efforts are carried out, emphasizing decentralized approaches that offer valuable services within communities, yielding long‐term advantages for the healthcare system. Considering the substantial quantity of these tests manufactured and used annually, a straightforward manufacturing process is proposed for highly sensitive carbon electrodes designed for antibody‐type biomarker sensors. This process, utilizing pad printing – an additive, low‐temperature, and cost‐effective method, coupled with plasma activation – has proven the electrodes capability to measure interferon gamma protein, a tuberculosis biomarker. Using electrochemical impedance spectroscopy, the electrodes display high sensitivity and are capable of measuring concentrations from 10 to 1000 pg mL−1 in undiluted serum within an hour. The sensor, utilizing solely a monolayer of antibodies, achieves a performance equivalent to that of a commercial standard sandwich ELISA tested in this study.
Growth and Photoresponse of WS2/MoSe2 Lateral Heterostructure
Authors: Sheng Mingyuan , Chang Xi , Mao Xiaojun , Gao Yang , Xuan Xiaoyang , Xie Haifen , Mu Haichuan , Niu Yueping , Gong Shangqing , Qian Min 
Published: May 2024

AbstractThe heterostructure of two‐dimensional transition metal dichalcogenide (TMDC) has garnered extensive attention, for the junction is the building block of a semiconductor device. However, the controllable synthesis of TMDC heterostructures of different transition metals and different chalcogen elements is still challenging because of the etching by atom substitution during the chemical vapor deposition (CVD) process. Here, a Mo─O transition state with lower energy is introduced to the edge of an as‐grown MoSe2 by using ultraviolet ozone treatment, to prevent the fast atom substitution of S for Se, and enable a stable growth of WS2/MoSe2 lateral heterostructure. A polymer‐free transfer method is developed based on capillary interaction, and atomic structure characterization confirms the high‐quality WS2/MoSe2 lateral heterostructure. The WS2/MoSe2 lateral heterostructure photodetector exhibits superior photoresponse compared to WS2 and MoSe2 devices, with a responsivity of 21.87 A W−1 and a detectivity of 4.2 × 1012 Jones at 350 nm. Kelvin probe force microscopy result reveals that the built‐in electric field within the heterojunction facilitates the effective separation of photogenerated electron‐hole pairs. This study carries profound implications for the CVD growth and polymer‐free transfer of TMDC heterostructures in photodetector applications.
Support for Baker's law: Facultative self‐fertilization ability decreases pollen limitation in experimental colonization
Authors: Makowski Hanna , Lamb Keric , Galloway Laura F. 
Published: May 2024

AbstractPremiseThe ability to self‐fertilize is predicted to provide an advantage in colonization because a single individual can reproduce and establish a next generation in a new location regardless of the density of mates. While there is theoretical and correlative support for this idea, the strength of mate limitation as a selective agent has not yet been delineated from other factors that can also select for self‐fertilization in colonization of new habitats. We used known mating‐system variation in the American bellflower (Campanula americana) to explore how plants’ ability to self‐fertilize can mitigate density‐dependent reproduction and impact colonization success.MethodsWe created experimental populations of single individuals or a small number of plants to emulate isolated colonization events. These populations were composed of plants that differed in their ability to self‐fertilize. We compared pollen limitation of the single individuals to that of small populations.ResultsExperimental populations of plants that readily self‐fertilize produced consistent seed numbers regardless of population size, whereas plants with lower ability to self‐fertilize had density‐dependent reproduction with greater seed production in small populations than in populations composed of a single individual.ConclusionsWe experimentally isolated the effect of mate limitation in colonization and found that it can select for increased self‐fertilization. We show the benefit of self‐fertilization in colonization, which helps to explain geographic patterns of self‐fertilization and shows support for Baker's law, a long‐held hypothesis in the field of mating‐system evolution.
Horizontal and vertical movements of the critically endangered Rostroraja alba in a coastal marine protected area
Authors: Kraft Sebastián , Winkler Alexander Claus , Abecasis David 
Published: May 2024

Abstract
Elasmobranchs are slow‐growing marine predators whose populations have declined due to their susceptibility to overfishing, especially affecting species of large size. Rostroraja alba is a large skate that is currently classified as critically endangered.
To address the effects of overfishing and promote the recovery of marine species and habitats, marine protected areas (MPAs) are a commonly implemented management strategy. A critical aspect of their success is using information on species' movement patterns; however, this is frequently not assessed for elasmobranchs.
In this study, acoustic telemetry was used to monitor 30 R. alba in the Professor Luís Saldanha Marine Park (Portugal) to assess their long‐term movements in the context of a coastal MPA and to evaluate the contribution of this kind of spatial management to the conservation of this species.
Immature individuals were more frequently captured than adults, which postulates this area as a potential nursery. The average residency was moderate and relatively consistent throughout the year, as was area use. No marked size or sex‐based differences in movement were noted. Individuals were more active and occurred in shallower waters at night and twilight, and occasional quick vertical movements into the water column, isolated or in sequence, were also noted.
In general, the LSMP seems to offer stable protection to immature R. alba, with no strong seasonal variation. These results provide relevant input for adaptive management measures of this and other similar MPAs and highlight the contribution of these efforts to the protection of nursery areas.
KDPM: Knowledge‐driven dynamic perception model for evacuation scene simulation
Authors: Tang Kecheng , Zhang Jiawen , Shen Yuji , Li Chen , He Gaoqi 
Published: May 2024

AbstractEvacuation scene simulation has become one important approach for public safety decision‐making. Although existing research has considered various factors, including social forces, panic emotions, and so forth, there is a lack of consideration of how complex environmental factors affect human psychology and behavior. The main idea of this paper is to model complex evacuation environmental factors from the perspective of knowledge and explore pedestrians' emergency response mechanisms to this knowledge. Thus, a knowledge‐driven dynamic perception model (KDPM) for evacuation scene simulation is proposed in this paper. This model combines three modules: knowledge dissemination, dynamic scene perception, and stress response. Both scenario knowledge and hazard source knowledge are extracted and expressed. The improved intelligent agent perception model is designed by adopting position determination. Moreover, a general adaptation syndrome (GAS) model is first presented by introducing a modified stress system model. Experimental results show that the proposed model is closer to the reality of real data sets.
The PI3K/Akt signaling axis and type 2 diabetes mellitus (T2DM): From mechanistic insights into possible therapeutic targets
Authors: Taheri Rana , Mokhtari Yazdan , Yousefi Amir‐Mohammad , Bashash Davood 
Published: May 2024

AbstractType 2 diabetes mellitus (T2DM) is an immensely debilitating chronic disease that progressively undermines the well‐being of various bodily organs and, indeed, most patients succumb to the disease due to post‐T2DM complications. Although there is evidence supporting the activation of the phosphoinositide 3‐kinase (PI3K)/Akt signaling pathway by insulin, which is essential in regulating glucose metabolism and insulin resistance, the significance of this pathway in T2DM has only been explored in a few studies. The current review aims to unravel the mechanisms by which different classes of PI3Ks control the metabolism of glucose; and also to discuss the original data obtained from international research laboratories on this topic. We also summarized the role of the PI3K/Akt signaling axis in target tissues spanning from the skeletal muscle to the adipose tissue and liver. Furthermore, inquiries regarding the impact of disrupting this axis on insulin function and the development of insulin resistance have been addressed. We also provide a general overview of the association of impaired PI3K/Akt signaling pathways in the pathogenesis of the most prevalent diabetes‐related complications. The last section provides a special focus on the therapeutic potential of this axis by outlining the latest advances in active compounds that alleviate diabetes via modulation of the PI3K/Akt pathway. Finally, we comment on the future research aspects in which the field of T2DM therapies using PI3K modulators might be developed.
Quality Inspection of Battery Separators by Partial Discharge Spectroscopy
Authors: Kumar Peeyush , Kasper Manuel , Kienberger Ferry , Gramse Georg 
Published: May 2024

AbstractQuality control is highly relevant for safety, sustainability and efficiency of the battery manufacturing process. An early and reliable detection of failures in the production chain is important. Here we present a method for detecting micrometric imperfections and contaminations on the battery separator before filling the battery stack with the electrolyte. We sense these irregularities by measuring an increase of partial discharges when applying between the battery electrodes potentials close, but still well below the breakdown voltage of the separator. We can distinguish different degrees and different types of contamination with a very high confidence. This is enabled by a throughout statistical analysis of the partial discharge events. The overall reliability of detecting a contaminated against the clean separator is 96 %. The technique, as implemented here, uses categorization procedures and machine learning algorithms to automate decision‐making and can accelerate the quality assessment process in pilot lines or small‐ manufacturing. Compared to other methods, like optical detection or full discharge measurements, the here presented approach is very reliable, simple to implement and virtually noninvasive.
Efficacy of texture and color enhancement imaging for the visibility and diagnostic accuracy of non‐polypoid colorectal lesions
Authors: Okumura Taishi , Hotta Kinichi , Imai Kenichiro , Ito Sayo , Kishida Yoshihiro , Takada Kazunori , Kawaguchi Daiki , Mori Yukihiro , Tanaka Yusuke , Tsushima Takahiro , Kawata Noboru , Maeda Yuki , Yoshida Masao , Yamamoto Yoichi , Minamide Tatsunori , Ishiwatari Hirotoshi , Sato Junya , Matsubayashi Hiroyuki , Ono Hiroyuki 
Published: May 2024

AbstractObjectiveA newly launched endoscopy system (EVIS X1, CV‐1500; Olympus) is equipped with texture and color enhancement imaging (TXI). We aimed to investigate the efficacy of TXI for the visibility and diagnostic accuracy of non‐polypoid colorectal lesions.MethodsWe examined 100 non‐polypoid lesions in 42 patients from the same position, angle, and distance of the view in three modes: white light imaging (WLI), narrow‐band imaging (NBI), and TXI. The primary outcome was to compare polyp visibility in the three modes using subjective polyp visibility score and objective color difference values. The secondary outcome was to compare the diagnostic accuracy without magnification.ResultsOverall, the visibility score of TXI was significantly higher than that of WLI (3.7 ± 1.1 vs. 3.6 ± 1.1; p = 0.008) and lower than that of NBI (3.7 ± 1.1 vs. 3.8 ± 1.1; p = 0.013). Color difference values of TXI were higher than those of WLI (11.5 ± 6.9 vs. 9.1 ± 5.4; p < 0.001) and lower than those of NBI (11.5 ± 6.9 vs. 13.1 ± 7.7; p = 0.002). No significant differences in TXI and NBI (visibility score: 3.7 ± 1.0 vs. 3.8 ± 1.1; p = 0.833, color difference values: 11.6 ± 7.1 vs. 12.9 ± 8.3; p = 0.099) were observed for neoplastic lesions. Moreover, the diagnostic accuracy of TXI was significantly higher than that of NBI (65.5% vs. 57.6%, p = 0.012) for neoplastic lesions.ConclusionsTXI demonstrated higher visibility than that of WLI and lower than that of NBI. Further investigations are warranted to validate the performance of the TXI mode comprehensively.
Comparative model of minimal spinal cord injury reveals a rather anti‐inflammatory response in the lesion site as well as increased proliferation in the central canal lining in the neonates compared to the adult rats
Authors: Ševc Juraj , Mochnacký Filip , Košuth Ján , Alexovič Matiašová Anna , Slovinská Lucia , Blaško Juraj , Bukhun Ivan , Holota Radovan , Tomori Zoltán , Daxnerová Zuzana 
Published: May 2024

AbstractSpinal cord injury (SCI) resulting from trauma decreases the quality of human life. Numerous clues indicate that the limited endogenous regenerative potential is a result of the interplay between the inhibitory nature of mature nervous tissue and the inflammatory actions of immune and glial cells. Knowledge gained from comparing regeneration in adult and juvenile animals could draw attention to factors that should be removed or added for effective therapy in adults. Therefore, we generated a minimal SCI (mSCI) model with a comparable impact on the spinal cord of Wistar rats during adulthood, preadolescence, and the neonatal period. The mechanism of injury is based on unilateral incision with a 20 ga needle tip according to stereotaxic coordinates into the dorsal horn of the L4 lumbar spinal segment. The incision should harm a similar amount of gray matter on a coronal section in each group of experimental animals. According to our results, the impact causes mild injury with minimal adverse effects on the neurological functions of animals but still has a remarkable effect on nervous tissue and its cellular and humoral components. Testing the mSCI model in adults, preadolescents, and neonates revealed a rather anti‐inflammatory response of immune cells and astrocytes at the lesion site, as well as increased proliferation in the central canal lining in neonates compared with adult animals. Our results indicate that developing nervous tissue could possess superior reparative potential and confirm the importance of comparative studies to advance in the field of neuroregeneration.
Damage evolution of surrounding sandstone rock under charging–discharging cyclic loading in the natural gas storage of abandoned mines based on the discrete element method
Authors: Ma Zhanguo , Sun Junyu , Gong Peng , Oh Erwin , Hu Jun , Zhang Ruichong 
Published: May 2024

AbstractGas storage in abandoned mines is one way to reuse waste space resources. The surrounding rock of gas storage reservoirs in underground roadways undergoes damage and deformation under the cyclic loading of gas charging and discharging, which can pose a risk to the safety of the reservoirs. This study establishes a true triaxial numerical model of rock mass with the discrete element method (DEM) and explores the crack evolution of surrounding rock of underground gas storage during cyclic loading and unloading. Also, a damage evolution model in numerical analysis considering residual deformation is developed to explain the experimental results. As was revealed, cyclic loading and unloading resulted in fatigue damage in the specimen and caused strength deterioration of the specimen. During the loading process, the uniformly distributed force chains of the rock mass redistributed, evolving gradually to mostly transverse force chains. This contributed to the appearance of blank areas in the force chains when through cracks appear. The ratio of tensile cracks to shear cracks gradually decreases and finally stabilizes at 7:1. The damage evolution model considering residual strain can be mutually verified with the numerical simulation results. Based on the DEM model, it was found that there was a certain threshold of confining pressure. When the confining pressure exceeded 30 MPa, the deformation to ductility of sandstone samples began to accelerate, with a greater residual strength. This study provides a theoretical basis for analyzing the long‐term mechanical behavior of surrounding rock of gas storage in abandoned mines.
Identification of suitable habitats and priority conservation areas under climate change scenarios for the Chinese alligator (Alligator sinensis)
Authors: Yang Liuyang , Ling Jiangnan , Lu Lilei , Zang Dongsheng , Zhu Yunzhen , Zhang Song , Zhou Yongkang , Yi Pingsi , Li En , Pan Tao , Wu Xiaobing 
Published: May 2024

AbstractAmphibians and reptiles, especially the critically endangered Chinese alligators, are vulnerable to climate change. Historically, the decline in suitable habitats and fragmentation has restricted the distribution of Chinese alligators to a small area in southeast Anhui Province in China. However, the effects of climate change on range‐restricted Chinese alligator habitats are largely unknown. We aimed to predict current and future (2050s and 2070s) Chinese alligator distribution and identify priority conservation areas under climate change. We employed species distribution models, barycenter migration analyses, and the Marxian model to assess current and future Chinese alligator distribution and identify priority conservation areas under climate change. The results showed that the lowest temperature and rainfall seasonality in the coldest month were the two most important factors affecting the distribution of Chinese alligators. Future predictions indicate a reduction (3.39%–98.41%) in suitable habitats and a westward shift in their distribution. Further, the study emphasizes that suitable habitats for Chinese alligators are threatened by climate change. Despite the impact of the Anhui Chinese Alligator National Nature Reserve, protection gaps persist, with 78.27% of the area lacking priority protected area. Our study provides crucial data for Chinese alligator adaptation to climate change and underscores the need for improved conservation strategies. Future research should refine conservation efforts, consider individual plasticity, and address identified limitations to enhance the resilience of Chinese alligator populations in the face of ongoing climate change.
Intrahepatic plasma cells, but not atypical memory B cells, associate with clinical phases of chronic hepatitis B
Authors: Osmani Zgjim , Villanueva Martin Arreola , Joseph‐Chazan Jasmin , Beudeker Boris J. , de Knegt Robert J. , Chung Raymond T. , Hacohen Nir , Aerssens Jeroen , Bollekens Jacques , Janssen Harry L. A. , Gehring Adam J. , Lauer Georg M. , Shalek Alex K. , van de Werken Harmen J. G. , Boonstra Andre 
Published: May 2024

AbstractStudies have traditionally focused on the role of T cells in chronic hepatitis B (CHB), but recent evidence supports a role for B cells. The enrichment of so‐called atypical memory (AtM) B cells, which show reduced signaling and impaired differentiation, is believed to be a characteristic feature of CHB, potentially contributing to the observed dysfunctional anti‐HBsAg B‐cell responses. Our study, involving 62 CHB patients across clinical phases, identified AtM B cells expressing IFNLR1 and interferon‐stimulated genes. Contrary to previous reports, we found relatively low frequencies of AtM B cells in the liver, comparable to peripheral blood. However, liver plasma cell frequencies were significantly higher, particularly during phases with elevated viral loads and liver enzyme levels. Liver plasma cells exhibited signs of active proliferation, especially in the immune active phase. Our findings suggest a potential role for plasma cells, alongside potential implications and consequences of local proliferation, within the livers of CHB patients. While the significance of AtM B cells remains uncertain, further investigation is warranted to determine their responsiveness to interferons and their role in CHB.
Understanding the Role of Controlled Environments for Producing Mycelium‐bound Composites: Advancing Circular Practices for Integrating Biotechnology into the Construction Industry
Authors: Derme Tiziano , Schwarze Francis W. M. R. , Dillenburger Benjamin 
Published: May 2024

AbstractThe architecture, engineering, and construction industry is undergoing a significant shift, steering buildings away from resource‐intensive processes toward becoming instruments for climate mitigation. In this transformative landscape, integrating circular bio‐based alternatives and reducing emissions through biotechnological and enzymatic processes have significant potential. Specifically, mycelium‐bound composites have emerged as renewable alternatives for new materials and added‐value wood products. Despite their numerous advantages, integrating these materials into current engineering practices presents challenges deriving from the complex nature of the material´s production process and the transfer from the laboratory to the industrial scale. In this regard, the design and engineering of novel controlled environments are fundamental in maintaining optimal growth conditions during material production. This, in turn, influences the overall material performance and potential use in construction.
Intimate partner violence and its associated factors among married women at Jeldu district, Central Ethiopia: A community‐based mixed cross‐sectional study
Authors: Yadesa Geremew , Bala Elias Teferi , Kolola Tufa , Solbana Lencho Kajela 
Published: May 2024

AbstractBackground and AimsIntimate partner violence is a global threat, regardless of any religious, cultural, or economic differences. Few studies have been conducted before in rural areas of Ethiopia. Therefore, this study aimed to assess the prevalence and associated factors of intimate partner violence among married women in Jeldu district.MethodsA community‐based mixed cross‐sectional study was conducted among 620 married women in Jeldu district, west Ethiopia. A systematic random sampling technique was employed to select study participants. The quantitative data were checked and entered into Epidata and STATA version 15.0 for analysis. Univariate and multivariate logistic regression was used to identify the associated factors of intimate partner violence. The finding of the quantitative study was triangulated with the findings of focused group discussion.ResultsSix hundred seven married women participated in the study making a response rate of 97.43%. The lifetime and past 12 months prevalence of intimate partner violence was 57.7% (95% confidence interval [CI]: 53.78%–61.62%) and 53.20% (95% CI: 49.28%–57.12%) respectively. Partner with lower education (adjusted odd ratio [AOR] = 3.64 (95% CI: 1.07–12.38), alcohol intake by partner (AOR = 1.92, 95% CI: 1.31–2.81), equal dominance on family affairs (AOR = 0.30, 95% CI: 0.18–0.51), and family size >5 (AOR = 4.54, 95% CI: 1.89–10.91) were factors significantly associated with intimate partner violence.ConclusionThe prevalence of intimate partner violence was relatively higher among married women study area. Partner's lower educational status, alcohol intake of the partner, dominance on family issues, and family size were factors associated with intimate partner violence. So, gender offices, and district and regional educational sectors should design appropriate strategies and work hard to tackle the problem.