bluuebunny/crossref_metadata_2025_split huggingface dataset:
Characterization of interaction between doxycycline and human serum albumin by capillary electrophoresis‐frontal analysis
Authors: Sun Hanwen , He Pan 
Published: June 2009

AbstractThe binding of doxycycline to HSA under simulated physiological conditions (pH 7.4, 67 mM phosphate, I=0.17, drug concentration 100 μM, HSA concentration up to 475 μM, 36.5°C) was studied by CE‐frontal analysis. The number of primary binding sites, binding constant and physiological protein‐binding percentage were 1.9, 1.51×103 M−1 and 59.80%, respectively. In addition, the thermodynamic parameters including enthalpy change (ΔH), entropy change (ΔS) and free energy change (ΔG) of the reaction were obtained in order to characterize the acting forces between doxycycline and HSA. Furthermore, to better understand the nature of doxycycline–HSA binding and to get information about potential interaction with other drugs, displacement experiments were performed. The results showed that doxycycline binds at site II of HSA.
Quantifying Western blots: Pitfalls of densitometry
Authors: Gassmann Max , Grenacher Beat , Rohde Bianca , Vogel Johannes 
Published: June 2009

AbstractAlthough Western blots are frequently quantified, densitometry is not documented and appears to be based merely on traditions and guesswork. Confirming previous experience, none of 100 randomly selected and systematically scanned most recent papers provided sufficient information on how Western blot results were translated into statistical values. The importance of such information, however, becomes evident from our correlations of plasma erythropoietin values of various mammals determined using RIA and Western blot densitometry. Different common densitometry procedures applied to the identical Western blot revealed p‐values of these correlations ranging from 0.000013 to 0.76 reflecting the necessity of a scientifically sound basis for densitometry of Western blots. At present, the current lack of any definitions in densitometry opens the door to uncontrollable acquisition of any desired p‐value. Here we provide data that define what should be considered, what avoided and what documented when quantifying Western blots.
A novel method developed for acetylcholine detection in royal jelly by using capillary electrophoresis coupled with electrogenerated chemiluminescence based on a simple reaction
Authors: Wei Wei , Wei Min , Kang Xuejun , Deng Huihua , Lu Zuhong 
Published: June 2009

AbstractA novel method for highly sensitive detection of acetylcholine in royal jelly was proposed by using CE coupled with electrogenerated chemiluminescence (ECL). Acetylcholine, which could not react with Tris(2,2′‐bipyridine)ruthenium(II) to strengthen its ECL signals, decomposed into trimethylamine and strengthened the ECL signals sharply when it was heated to its melting point. This reaction needed no additional reagent and it was mild, simple, stable and rapid, without any side reaction. By combining the above process with CE separation technique, trimethylamine in royal jelly was completely separated from interfering substances and was successfully detected within 4 min. The limit of detection for acetylcholine was found to be 6.3×10−8 g/mL with a signal‐to‐noise ratio of 3:1. Acetylcholine in the royal jelly was detected to be 912±58 μg/g. The recoveries of acetylcholine chloride in the sample were in the ranges of 92–106%. The coefficients of variation for intra‐day and inter‐day reproducibility were equal to or less than 4.9 and 6.8%, respectively.
Cyclodextrin‐modified capillary electrophoresis for achiral and chiral separation of ergostane and lanostane compounds extracted from the fruiting body of Antrodia camphorata
Authors: Majid Ehsan , Male Keith B. , Tzeng Yew‐Min , Omamogho Jesse O. , Glennon Jeremy D. , Luong John H. T. 
Published: June 2009

AbstractA CD‐modified capillary electrophoretic method has been developed for achiral and chiral analysis of seven bioactive compounds isolated from the fruiting body of Antrodia camphorata. Such important target analytes exhibit similar chemical structures and are known for their diverse properties including antioxidant and anticancer effects. The analytes were separated in 25 min using a pH 9.3, 20 mM sodium borate buffer containing 20 mM methyl‐β‐CD and 30 mM sulfobutylether‐β‐CD. With the exception of the optical isomer pairs (antcin B or zhankuic acid A, zhankuic acid C, and antcin A), the remaining bioactive compounds including the chiral pair antcin C were baseline‐separated. Analysis time was noticeably longer to baseline separate all of the above chiral pairs (∼38 min) by adding 5% DMF to the running buffer. The migration order was reversed compared with the HPLC elution. More hydrophobic compounds complexed favorably with methyl‐β‐CD and emerged earlier in the electropherogram than their more hydrophilic counterparts which were strongly associated with sulfobutylether‐β‐CD. The simple capillary electrophoretic method developed was applicable for rapid separation and characterization of several important bioactive compounds isolated from the fruiting body of A. camphorata.
A capillary electrophoretic system based on a novel microemulsion for the analysis of coenzyme Q10 in human plasma by electrokinetic chromatography
Authors: Lucangioli Silvia , Sabrina Flor , Mario Contin , Valeria Tripodi 
Published: June 2009

AbstractA new analytical method for determination of coenzyme Q10 (2,3‐dimethoxy‐5‐methyl‐6‐decaprenyl‐1,4‐benzoquinone, CoQ10) in human plasma was developed based on CE using a double tensioactive microemulsion. CoQ10 was quantitatively extracted into 1‐propanol/hexane and quantified by MEEKC. The microemulsion was prepared by mixing 1.4% w/w sodium bis(2‐ethylhexyl) sulfosuccinate, 4% w/w cholic acid, 1% w/w octane, 8.5% w/w butanol, 0.1% w/w PVA and 85% w/w 10 mM Tris buffer at pH 9.0. The optimized electrophoretic conditions included the use of an uncoated silica capillary of 60 cm total length and 75 μm id, an applied voltage of 20 kV, room temperature and 214 nm ultraviolet detection. Selectivity, linearity, LOD, LOQ, precision and accuracy were evaluated as the parameters of validation. Owing to its simplicity and reliability, the proposed method can be an advantageous alternative to the traditional methodology for the quantitation of CoQ10 in human plasma with good accuracy and precision.
Plasmonic nanoprobes for SERS biosensing and bioimaging
Authors: Vo‐Dinh Tuan , Wang Hsin‐Neng , Scaffidi Jonathan 
Published: June 2009

AbstractThis article provides an overview of the development and application of plasmonic nanoprobes developed in our laboratory for biosensing and bioimaging. We describe the use of plasmonics surface‐enhanced Raman scattering (SERS) gene probes for the detection of diseases using DNA hybridization to target biospecies (HIV gene, breast cancer genes etc.). For molecular imaging, we describe a hyperspectral surface‐enhanced Raman imaging (HSERI) system that combines imaging capabilities with SERS detection to identify cellular components using Raman dye‐labeled silver nanoparticles in cellular systems The detection of specific target DNA sequences associated with breast cancer using “molecular sentinel” nanoprobes and the use of a plasmonic nanosensor to monitor pH in single cells are presented and discussed. (© 2010 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim)
Public reporting of hospital quality: Recommendations to benefit patients and hospitals
Authors: Rothberg Michael B. , Benjamin Evan M. , Lindenauer Peter K. 
Published: June 2009

AbstractPublic reporting of hospital performance holds tremendous promise for improving the care provided by hospitals. To date, however, consumers have failed to embrace public reporting, despite considerable efforts to promote it. We review a number of reasons that public reporting has failed to live up to expectations, and we make 10 recommendations to improve the value of public reporting for both patients and hospitals. We also review 3 leading performance reporting programs to evaluate how well they adhere to these recommendations. Journal of Hospital Medicine 2009;4:541–545. © 2009 Society of Hospital Medicine.
Late Pleistocene and Holocene glaciation of the Fish Lake valley, northeastern Alaska Range, Alaska
Authors: Young Nicolás E. , Briner Jason P. , Kaufman Darrell S. 
Published: June 2009

AbstractWe reconstructed a chronology of glaciation spanning from the Late Pleistocene through the late Holocene for Fish Lake valley in the north‐eastern Alaska Range using 10Be surface exposure dating and lichenometry. After it attained its maximum late Wisconsin extent, the Fish Lake valley glacier began to retreat ca. 16.5 ka, and then experienced a readvance or standstill at 11.6 ± 0.3 ka. Evidence of the earliest Holocene glacial activity in the valley is a moraine immediately in front of Little Ice Age (LIA) moraines and is dated to 3.3–3.0 ka. A subsequent advance culminated at ca. AD 610–900 and several LIA moraine crests date to AD 1290, 1640, 1860 and 1910. Our results indicate that 10Be dating from high‐elevation sites can be used to help constrain late Holocene glacial histories in Alaska, even when other dating techniques are unavailable. Close agreement between 10Be and lichenometric ages reveal that 10Be ages on late Holocene moraines may be as accurate as other dating methods. Copyright © 2009 John Wiley & Sons, Ltd.
Tribological performance of ionised vegetable oils as lubricity and fatty oiliness additives in lubricants and fuels
Authors: Roegiers Michel , Zhmud Boris 
Published: June 2009

AbstractLubricity and fatty oiliness additives, also known as friction modifiers in the tribological vocabulary, are steadily gaining acceptance from lubrication engineers and lubricant formulators. The present communication describes how such additives function in various tribosystems and which parameters control lubricity of finished formulations. Extensive experimental data are presented to demonstrate the outstanding tribological performance of bio‐based lubricity and fatty oiliness additives produced by ElektrionizationTM of vegetable feedstocks. Featuring a unique combination of viscosity and polarity, ionised vegetable oils form sufficiently thick and resilient protective layers by adsorption to rubbing surfaces. It is shown that, unlike extreme pressure additives, which act when a direct asperity–asperity contact occurs in the boundary lubrication regime, ionised vegetable oils function by postponing the onset of the boundary lubrication regime. Copyright © 2009 John Wiley & Sons, Ltd.
Large‐Scale Oriented Assembly of Nanoparticles in Diblock Copolymer Templates under Electric Fields
Authors: Yan Li‐Tang , Schoberth Heiko G. , Böker Alexander 
Published: June 2009

AbstractWe perform cell dynamics simulations to study the assembly of nanoparticles in diblock copolymer templates under electric fields. Our simulations predict that the nanoparticles directed by the diblock copolymer templates can assemble into various oriented structures, e.g., lamella, alternating lamella (for two types of particles), and cylinder on the macroscopic scale by selecting appropriate parameters when an electric field is applied. Our results demonstrate that there exists a strong correlation between the ordering kinetics of nanoparticles and the evolution of block copolymer domains under electric fields. The described method can provide a new route to realize ordered and anisotropic arrays of nanoparticles on a macroscopic scale.magnified image
Semiconducting Oligo‐(Aryleneethynylene)s with Coplanarity of Main Chain and Tetrathiafulvalene (TTF) Side Chains: Synthesis, Self‐Assembly, and Conductive Properties
Authors: Hou Yanhui , Yang Min , Wan Xiangjian , Mao Jie , Ma Yanfeng , Huang Yi , Chen Yongsheng 
Published: June 2009

AbstractTwo oligo‐(aryleneethynylene)s, with coplanarity of main chain and tetrathiafulvalene (TTF) side chains, have been prepared and characterized. The X‐ray diffractions (XRDs) show that their π‐extended coplanar backbones can form continuous π‐stacking. For the two oligomers, one stacks in the inter‐digitation packing mode; another stacks in the end‐to‐end packing mode. Cyclic voltammetries reveal that the two oligomers have almost the same reversible electroactive properties. The TTF units of the two oligomers can be oxidized to TTF.1+ by Fe(bpy)3(PF6)3 (bpy = 2,2′‐bipyridine). The band gaps, deduced from UV‐Vis absorption spectra, are 1.92 and 2.03 eV, respectively. The conductivities of the two oligomers are 1 × 10−5 and 6 × 10−8 S · cm−1 at room temperature. The charge transfer (CT) complexes of the oligomers and tetracyanoquinodimethane (TCNQ) exhibit higher conductivity up to 0.2 S · cm−1.magnified image
Cationic Photopolymerization in Presence of Functionalized Silica Nanoparticles
Authors: Chemtob Abraham , Croutxé‐Barghorn Céline , Soppera Olivier , Rigolet Séverinne 
Published: June 2009

AbstractThe present study focuses on the synthesis of various silica‐filled polymer nanocomposites via cationic photopolymerization. The first part describes the chemical modification of fumed silica nanoparticles and their subsequent incorporation in three types of epoxy resins. 29Si CP MAS NMR spectroscopy and thermogravimetric analysis (TGA) enabled to characterize the surface structural composition of silica while dynamic light scattering (DLS) was employed to assess their degree of dispersion. In addition, the photopolymerization kinetics were investigated using real‐time Fourier transform infrared spectroscopy (RT‐FTIR). The capacity of reinforcement of the resulting nanocomposite films were explored in regard to modulus, glass transition behaviour, abrasion resistance and hardness.magnified image
Distortion engineering in turning processes with standard clamping systems
Authors: Grote C. , Brinksmeier E. , Garbrecht M. 
Published: June 2009

AbstractDuring turning of cylindrical workpieces with different diameters the industry often uses standard clamping systems. These systems are economic and able to clamp different diameters. Their benefit is that valuable time for jaw changing may be saved. Therefore a special interest lies in studies which allow the minimization of distortion by using these universal clamping systems. In this study a method is presented using three‐jaw‐chucks to minimize the wallthickness‐deviation of bearing rings caused by the turning process. The experimental approach considers a specific clamping sequence in combination with a variation of the clamping pressure. Additionally the use of a form‐locking clamping system, which allows a clear decrease of the necessary clamping pressure, will be examined. As a major result an optimized specific clamping sequence is presented, using segment jaws which will minimize the distortion caused by a varying depth of cut on the outer side of bearing rings.
The influence of carburizing on size and shape changes
Authors: Clausen B. , Surm H. , Hoffmann F. , Zoch H.‐W. 
Published: June 2009

AbstractSince seven years the Collaborative Research Center SFB 570 deals successfully with the ascertainment, description, and simulation of distortion. This paper aims to subjoin how carburizing and with it the case hardening process affects the already described effects of distortion and how the effects interact with each other. Due to carburizing a gradient in chemical composition is adjusted in the component. This affects not only a change in mechanical properties but also in the transformation behaviour. The local properties result in a locally varying influence on internal stresses during heating and cooling processes and deformations. Shape changes can therefore be massively influenced by the carbon profile. So far investigations have been carried out with disks and recessed shafts. Interactions to carburizing parameters appear between the intensity of segregations, the deformation parameters, stacking arrangements und quenching parameters.
Use of X‐ray diffraction and micromagnetic methods for a complete characterization of residual stress states in manufactured parts
Authors: Epp J. , Hirsch T. , de Souza Rosendo T. 
Published: June 2009

AbstractAs machining induced residual stress states are an important factor for the distortion of parts during following heat treatments, a complete characterization of the components residual stress state is required. For a gain of time, magnetic and micromagnetic analysis has been compared with X‐ray diffraction analysis. Cylindrical and tapered ball bearing rings made from AISI52100 steel with various and inhomogeneous residual stress states have been investigated. Reliable results and good agreement between X‐Ray diffraction data and residual stresses calculated form the magnetic and micromagnetic analysis can be obtained with the use of a calibration for each single component. An important gain of time can be achieved with the combined use of X‐ray diffraction analysis for the calibration and the micromagnetic technique. However, local variations of the residual stress may not be detected in localized zones smaller than the sensor. A global calibration with one calibration file for eight rings still needs some optimization.
Stress‐strain behaviour of the case hardening steel 20MnCr5 regarding different microstructures
Authors: Dalgic M. , Irretier A. , Zoch H.‐W. 
Published: June 2009

AbstractWithin the Collaborative Research Centre SFB 570 distortion is treated as a system attribute. This means that it has to be investigated during the entire processing of a component. Simulation is a powerful tool to predict and to take action on reducing distortion, but reliable materials data are one of the limiting factors of these simulations.Within these investigation, the material behaviour of the case hardening steel 20MnCr5 is described by thermo physical material values, parameters regarding the transformation and by the stress‐strain curve. Besides the temperature the microstructure has a substantial influence on the deformation curve. The subject of this paper is a description of the stress‐strain behaviour as a function of microstructure in dependence of the temperature and the carbon content by suitable equations. Therefore, tensile tests were carried out regarding different carbon contents for stable microstructures, but also conditions like the metastable austenite.
Anisotropic transformation strain and transformation plasticity: two corresponding effects
Authors: Hunkel M. 
Published: June 2009

AbstractDistortion is an effect of inhomogeneous strain behaviour which can be caused by plasticity resulting from thermal or transformation stresses, inhomogeneous phase transformations, or inhomogeneous anisotropic strains during phase transformations. Anisotropic strains during phase transformations incorporate anisotropic transformation strain as well as transformation plasticity. Both effects will be discussed in this paper in a corresponding manner. This includes a theoretical derivation, experiments for determination of these effects, and experiments and simulations on workpieces.
Handedness does not predict side of onset of motor symptoms in Parkinson's disease
Authors: Štochl Jan , Hagtvet Knut A. , Brožová Hana , Klempíř Jiří , Roth Jan , Růžička Evžen 
Published: June 2009

AbstractThis study focused on the relationship between the asymmetry of initial motor symptoms of Parkinson's disease (PD) and premorbid handedness of patients. Structural equation modeling has been used for this purpose. The survey consisting of validated items measuring handedness and questions related to side of occurrence of initial symptoms was administered to 472 patients with PD [277 men, 195 women, mean age 66.5 (9.3), mean duration of the disease 10 (6.1) years]. The unidimensional model of handedness fits the data well (χ2 = 37.86, df = 20, P = 0.009, Root Mean Square Error of Approximation = 0.044, Comparative Fit Index = 1.00, Standardized Root Mean Square Residual = 0.042) and side of initial motor symptoms is not significantly related to the factor of handedness (r =0.11, SE = 0.07, P = 0.14). In contrast to several other studies, the results indicate that the side of first occurrence of PD signs cannot be predicted from premorbid handedness of patients. © 2009 Movement Disorder Society
Tissue distribution and elimination of sesaminol triglucoside and its metabolites in rat
Authors: Jan Kuo‐Ching , Hwang Lucy Sun , Ho Chi‐Tang 
Published: June 2009

AbstractSesame exhibits many beneficial physiological effects, which are mostly related to its lignan compounds, such as sesaminol glucosides. This investigation studies the distribution and elimination of sesaminol triglucoside from sesame in Sprague Dawley (SD) rats. In order to investigate the distribution of sesaminol triglucoside (p.o. 500 mg/kg) in SD rats, the changes in concentration of sesaminol triglucoside and its metabolites were determined in tissues and plasma within 24 h period after tube‐feeding to SD rats. Results showed that sesaminol triglucoside may be deglycosylated to form sesaminol first by intestinal microflora and then incorporated via lymphatic absorption into the cardiovascular system, transported to other tissues. The concentrations of sesaminol triglucoside and its metabolites in rectum, caecum, colon, and small intestines are higher than those in liver, lung, kidney, and heart. Its concentration in brain is low but detectable. Glucuronidation and sulfation was the main metabolic pathway for sesaminol in urine, and fecal elimination was a major route of elimination. From LC/MS/MS analysis of rat organs, sesaminol triglucoside can be converted to mammalian lignans, enterodiol (END), and enterolactone (ENL), by rat intestinal microflora. In the plasma, concentrations of END and ENL were 5.9 ± 0.2 and 5.5 ± 0.2 μmol/mL, respectively.
Interactions between ABC‐transport proteins and the secondaryFusariummetabolites enniatin and beauvericin
Authors: Dornetshuber Rita , Heffeter Petra , Sulyok Michael , Schumacher Rainer , Chiba Peter , Kopp Stephan , Koellensperger Gunda , Micksche Michael , Lemmens‐Gruber Rosa , Berger Walter 
Published: June 2009

AbstractEnniatins (ENN) and beauvericin (BEA) exert cytotoxic properties. Here, we observed that their impact on Ca2+‐homeostasis can be reversed by exogenous ATP. Thus, we investigated whether membrane‐located ATP‐binding cassette (ABC) transporters influence ENNs‐ and BEA‐induced cytotoxicity. In short‐term exposure assays breast cancer resistance protein (ABCG2)‐overexpression weakly but significantly reduced the cytotoxic activity of BEA but not ENNs. In contrast, multidrug resistance‐associated protein‐1 (ABCC1)‐ andP‐glycoprotein (ABCB1)‐overexpression was not protective under identical conditions. ABCG2‐mediated resistance against BEA was reversible by ABCG2 modulators. In long‐term exposure assays, ABCG2 and ABCB1 significantly protected against ENNs‐ and to a lesser extent BEA‐induced cytotoxicity. Moreover, both fusariotoxins potently inhibited the ABCG2‐ and ABCB1‐mediated efflux of specific fluorescent substrates, with BEA being more effective. Additionally, ATPase and photoaffinity‐labelling assays proofed interaction of both substances with ABCG2 and ABCB1. Remarkably, 2 years selection of KB‐3‐1 cells against both fusariotoxins resulted only in two‐fold ENNs but negligible BEA resistance. Interestingly, the selected sublines displayed upregulation of multidrug resistance proteins and crossresistance to other chemotherapeutics. Summarizing, ABCG2 and ABCB1 slightly but significantly protect human cells against ENNs‐ and BEA‐induced cytotoxicity. However, both mycotoxins potently interact with ABCB1 and ABCG2 transport functions suggesting influences on bioavailability of xenobiotics and pharmaceuticals.