bluuebunny/crossref_metadata_2025_split huggingface dataset:
Frequented species of field fungi on wheat and their potential production of toxic metabolites
Authors: Mašková Zuzana , Tančinová Dana , Barboráková Zuzana , Mokrý Michal 
Published: February 2011

The aim of this study was to monitor isolates of Alternaria and Fusarium species, isolated from Slovak wheat grains in 2006 - 2008, for ability to produce mycotoxins and to estimate a potential contamination risk of wheat grains by mycotoxins. Toxinogenity of isolates was analyzed by means of thin layer chromatography (TLC) and liquid chromatography coupled to tandem mass spectrometry (LC/MS/MS). A total of 302 Alternaria species (A. alternata, A. arborescens, A. infectoria, A. tenuissima) were tested by TLC method and a total of 238 Fusarium species (F. acuminatum, F. avenaceum, F. crookwellense, F. culmorum, F. equiseti, F. graminearum, F. langsethiae, F. oxysporum, F. poae, F. proliferatum, F. semitectum, F. solani, F. sporotrichioides, F. subglutinans, F. tricinctum, F. verticillioides) were analyzed by TLC as well as LC/MS/MS method. All Alternaria sp. strains, excepting A. infectoriastrains, showed high potention to produce altenuen, alternariol and alternariol monomethylether. None of A. infectoriaspecies strains produced any mycotoxins analyzed in this study. Fusarium sp. strains demonstrated, according to toxicology specificity, ability to produce trichothecenes (deoxynivalenol, diacetoxyscirpenol, fusarenone X, HT-2 toxin, monoacetoxyscirpenol, neosolaniol, nivalenol, T-2 toxin), fumonisins, zearalenones, moniliformine and rarely mentioned toxins as aurofusarine, beauvericine, enniatins, equisetin and chlamydosporol. High potential production of mycotoxins and wide spectrum of toxic metabolites represent high risk of toxins production in real field conditions.
Microbial quality of honey mixture with pollen
Authors: Kňazovická Vladimí­ra , Kačániová Miroslava , Dovičičová Mária , Melich Martin , Kadási-Horáková Miriam , Barboráková Zuzana , Mareček Ján 
Published: February 2011

The aim of this study was evaluation of microbial quality in raw materials (honey, pollen) and evaluation of microbial quality in honey mixture with pollen (2.91 % and 3.85 %) and also dynamics of microbial groups in honey mixtures with pollen after 14 days storage at the room temperature (approximately 25 °C) and in cold store (8 °C). We used dilution plating method for testing of samples. Detections of total plate microbial count (aerobic and anaerobic microorganisms), sporulating bacteria, coliform bacteria, Bifidobacterium sp., Lactobacillus sp. and microscopic fungi were performed. In general, counts of microorganisms decreased in honey mixture with pollen compared to raw pollen and these counts increased compared to natural honey. Total plate count was 5.37 log KTJ.g-1 in pollen; 1.36 log KTJ.g-1 in honey; 2.97 log KTJ.g-1 in honey mixture with 2.91 % pollen and 2.04 log KTJ.g-1 in honey mixture with 3.85 % pollen. Coliform bacteria were detected in pollen (1.77 log KTJ.g-1). Then, we found coliform bacteria in one sample of honey mixtures with pollen (2.91 %) - 1.00 log KTJ.g-1.Bifidobacterium species were detected only in raw pollen. We did not findLactobacillus sp. in any of the samples. Microscopic fungi were detected on two cultivating media. Yeasts were present in pollen sample (average 5.39 log KTJ.g-1), honey mixture with 2.91 % pollen (average 2.51 log KTJ.g-1) and honey mixture with 3.85 % pollen (average 1.58 log KTJ.g-1). Filamentous microscopic fungi were detectable in pollen (average 3.38 log KTJ.g-1), in honey (only on one medium: 1.00 log KTJ.g-1), in honey mixture with 2.91 % pollen (average 1.15 log KTJ.g-1) and in honey mixture with 3.85 % pollen (1.71 %). Raw pollen contained microscopic fungi as Absidiasp., Mucor sp., Alternaria sp. andEmericella nidulans. Honey mixture with 2.91 % pollen after storage (14 days) contained lower microbial counts when compared with the sample analyzed at the beginning, beside sporulating bacteria and filamentous microscopic fungi in sample stored at 8 °C. We recorded growth of anaerobic microorganisms in honey mixture with 3.85 % pollen after storage (8 °C, 25 °C / 14 days).
The influence of first wort part and afterworts on saccharification of wort
Authors: Tóth Žigmund , Dráb Štefan , Frančáková Helena , Lí­šková Miriam , Návojská Jana 
Published: February 2011

Wort is a basic product of mashing, which forms the first intermediate in beer production and constitute the base of its final value. For qualitative value wort has the greatest impact grist per brew, which is a description of materials, they bring to brew extract and determine its the volume and concentration. The main component grist per brew for light and dark beers is stored pale malt and possibly a smaller proportion of adjuncts. The aim of our work was to assess the qualitative parameters of malt in terms of content extract and its impact on the amount of produced the first wort part and afterwort and their qualitative values expressed in % saccharification and volumes. We measured 3 types of malts with the content of the extract 75.2%, 76.1%, 77.2% in the original sample, which determined mainly reached saccharification of first part wort and other afterwort parts one and two. In terms attained of saccharification it was necessary to use on sparge of spent grains at afterwort number two only the amount of water, which would be not affect the total saccharification of wort and its qualitative parameters.
Analyse of traits of milk production in dairy cows
Authors: Bujko Jozef , Kocman Roman , Žitný Julius , Trakovická Anna , Hrncar Cyril 
Published: February 2011

When evaluating milk performance indicators, we mainly focused on dairy cows of the Holstein breed and its cross-breeds. Within the Holstein breed and its cross-breeds we evaluated 68 dairy cows, which produced on average 6447.63 kg of milk, 272.42 kg of fat, 210.73 kg of proteins and 307.79 kg of lactose. By means of a device LACTOSCAN 90 we defined basic milk components in our samples in laboratory conditions on the basis of set working procedures. Average fat content in our samples stood at 3.87 g/100 g. Average proteins content in our samples stood at 3.36 g/100 g. Average lactose content stood at 4.96 g/100 g. When assessing milk technological qualities we determined the average milk acidity value in our samples, which was 5.85 oSH. The average thermostability value of the tested samples was 2.75. In the milk cheeseability test the shortest time needed for sample precipitation was detected in samples No. 4 (42 s.), No. 13 (185 s.) and No.11 (190 s.).
Treatment-Related Mortality With Bevacizumab in Cancer Patients
Authors: Ranpura Vishal , Hapani Sanjaykumar , Wu Shenhong 
Published: February 2011

ContextFatal adverse events (FAEs) have been reported in cancer patients treated with the widely used angiogenesis inhibitor bevacizumab in combination with chemotherapy. Currently, the role of bevacizumab in treatment-related mortality is not clear.ObjectiveTo perform a systematic review and meta-analysis of published randomized controlled trials (RCTs) to determine the overall risk of FAEs associated with bevacizumab.Data SourcesPubMed, EMBASE, and Web of Science databases as well as abstracts presented at American Society of Clinical Oncology conferences from January 1966 to October 2010 were searched to identify relevant studies.Study Selection and Data ExtractionEligible studies included prospective RCTs in which bevacizumab in combination with chemotherapy or biological therapy was compared with chemotherapy or biological therapy alone. Summary incidence rates, relative risks (RRs), and 95% confidence intervals (CIs) were calculated using fixed- or random-effects models.Data SynthesisA total of 10 217 patients with a variety of advanced solid tumors from 16 RCTs were included in the analysis. The overall incidence of FAEs with bevacizumab was 2.9% (95% CI, 2.0%-4.2%). Compared with chemotherapy alone, the addition of bevacizumab was associated with an increased risk of FAEs, with an RR of 1.33 (95% CI, 1.02-1.73; P = .04; incidence, 2.9% vs 2.2%). This association varied significantly with chemotherapeutic agents (P = .006) but not with tumor types (P = .06) or bevacizumab doses (P = .32). Bevacizumab was associated with an increased risk of FAEs in patients receiving taxanes or platinum agents (RR, 3.49; 95% CI, 1.82-6.66; incidence, 3.3% vs 1.0%) but was not associated with increased risk of FAEs when used in conjunction with other agents (RR, 0.83; 95% CI, 0.37-1.85; incidence, 1.6% vs 1.6%). The most common causes of FAEs were hemorrhage (23.5%), neutropenia (12.2%), and gastrointestinal tract perforation (7.1%).ConclusionIn a meta-analysis of RCTs, bevacizumab in combination with chemotherapy or biological therapy, compared with chemotherapy alone, was associated with increased treatment-related mortality.
Cosmological constant from quarks and torsion
Authors: Popławski N.J. 
Published: February 2011

AbstractWe present a simple and natural way to derive the observed small, positive cosmological constant from the gravitational interaction of condensing fermions. In the Riemann‐Cartan spacetime, torsion gives rise to the axial–axial vector four‐fermion interaction term in the Dirac Lagrangian for spinor fields. We show that this nonlinear term acts like a cosmological constant if these fields have a nonzero vacuum expectation value. For quark fields in QCD, such a torsion‐induced cosmological constant is positive and its energy scale is only about 8 times larger than the observed value. Adding leptons to this picture could lower this scale to the observed value.
Are female researchers less cited? A large‐scale study of Norwegian scientists
Authors: Aksnes Dag W. , Rorstad Kristoffer , Piro Fredrik , Sivertsen Gunnar 
Published: February 2011

AbstractNumerous studies have shown that female scientists tend to publish significantly fewer publications than do their male colleagues. In this study, we have analyzed whether similar differences also can be found in terms of citation rates. Based on a large‐scale study of 8,500 Norwegian researchers and more than 37,000 publications covering all areas of knowledge, we conclude that the publications of female researchers are less cited than are those of men, although the differences are not large. The gender differences in citation rates can be attributed to differences in productivity. There is a cumulative advantage effect of increasing publication output on citation rates. Since the women in our study publish significantly fewer publications than do men, they benefit less from this effect. The study also provides results on how publication and citation rates vary according to scientific position, age, and discipline.
Comparison of Prognostic Outcome Between Left Circumflex Artery–Related and Right Coronary Artery–Related Acute Inferior Wall Myocardial Infarction Undergoing Primary Percutaneous Coronary Intervention
Authors: Chen Yung‐Lung , Hang Chi‐Ling , Fang Hsiu‐Yu , Tsai Tzu‐Hsien , Sun Cheuk‐Kwan , Chen Chien‐Jen , Chen Shyh‐Ming , Yang Cheng‐Hsu , Hsieh Yuan‐Kai , Wu Chiung‐Jen , Fu Morgan , Yip Hon‐Kan 
Published: February 2011

AbstractBackgroundThis study evaluated the 30‐day clinical outcome of patients with acute inferior wall ST‐elevation myocardial infarction (AIW‐STEMI) from occlusion of the left circumflex artery (LCX) vs the right coronary artery (RCA) undergoing primary percutaneous coronary intervention (PCI).HypothesisThe clinical outcomes of AIW‐STEMI caused by RCA and LCX occlusion may be different for patients undergoing primary PCI.MethodsBetween May 2002 and September 2009, 646 consecutive patients with AIW‐STEMI undergoing primary PCI were enrolled. Of these patients, 520 (80.5%) with AIW‐STEMI caused by RCA occlusion were categorized into group 1, whereas the remaining 126 (19.5%) whose AIW‐STEMI was caused by LCX occlusion served as group 2.ResultsThe results demonstrated that the frequency of advanced congestive heart failure, respiratory failure requiring mechanical ventilatory support, and 30‐day mortality were remarkably higher in group 2 than in group 1 (all P < 0.01). Conversely, the incidence of right ventricular infarction and complete heart block were notably higher in group 1 than in group 2 (all P < 0.001). Additionally, the peak level of creatine kinase‐isoenzyme MB was significantly higher, whereas the left ventricular ejection fraction was notably lower in group 2 than in group 1. Multivariate analysis demonstrated that advanced CHF, high serum creatinine level, low systolic blood pressure, low left ventricular ejection fraction, and LCX as the infarct‐related artery were significantly and independently predictive of 30‐day mortality (all P < 0.05).ConclusionsThe 30‐day prognostic outcome was less favorable in LCX‐related AIW‐STEMI compared with RCA‐related AIW‐STEMI undergoing primary PCI. Copyright © 2011 Wiley Periodicals, Inc.Chi‐Ling Hang, MD, contributed equally in this study with Yung‐Lung Chen, MD. Morgan Fu, MD, contributed equally in this study with Hon‐Kan Yip, MD.The authors have no funding, financial relationships, or conflicts of interest to disclose.
Circulating Soluble Lectin‐Like Oxidized Low‐Density Lipoprotein Receptor‐1 Levels Are Associated With Angiographic Coronary Lesion Complexity in Patients With Coronary Artery Disease
Authors: Zhao Zi‐Wen , Zhu Xue‐Li , Luo Yu‐Kun , Lin Chao‐Gui , Chen Liang‐Long 
Published: February 2011

AbstractBackgroundAngiographic coronary lesion complexity has been reported to predict plaque vulnerability. It is important to develop a noninvasive blood biomarker for accurate prognostication of angiographically complex lesions in patients with coronary artery disease (CAD).HypothesisSerum soluble lectin‐like oxidized low‐density lipoprotein receptor‐1 (sLOX‐1) levels may be correlated with coronary lesion complexity in patients with CAD.MethodsWe measured serum sLOX‐1 levels in 180 consecutive patients undergoing coronary angiography for the evaluation of CAD. Coronary lesions were classified as simple or complex lesions based on coronary plaque morphology.ResultsStable CAD patients with complex lesions (n = 50) had significantly higher serum sLOX‐1 levels than those with simple lesions (n = 72), at 0.914 ng/mL (range, 0.489–1.296 ng/mL) vs 0.426 ng/mL (range, 0.195–1.075 ng/mL), respectively, P < 0.01. Multivariate logistic regression analysis revealed that sLOX‐1 levels were independently associated with the presence of complex lesions in patients with stable CAD (odds ratio [OR]: 1.964, 95% confidence interval [CI]: 1.149–3.356, P < 0.05). Among patients with acute coronary syndrome (n = 58), who had significantly higher circulating sLOX‐1 levels than stable CAD patients (n = 122) at 1.610 ng/mL (range, 0.941–2.264 ng/mL) vs 0.579 ng/mL (range, 0.265–1.172 ng/mL), respectively, P < 0.01, sLOX‐1 levels were independently associated with the presence of multiple complex coronary lesions (OR: 1.967, 95% CI: 1.075–3.600, P < 0.05).ConclusionsSerum sLOX‐1 levels were associated with complex lesions that might predict vulnerable plaques. This study suggested sLOX‐1 might be a useful biomarker of coronary plaque vulnerability in patients with CAD. © 2011 Wiley Periodicals, Inc.This work is supported in part by the Science Research Foundation of the Ministry of Health & United Fujian Provincial Health and Education Project for Tackling Key Research, People's Republic of China (No. WKJ2008‐2‐62), and by the key program of scientific research of Fujian Medical University (09ZD011). The authors have no other funding, financial relationships, or conflicts of interest to disclose.
Effect of the histone deacetylase inhibitor SNDX‐275 on Fas signaling in osteosarcoma cells and the feasibility of its topical application for the treatment of osteosarcoma lung metastases
Authors: Koshkina Nadezhda V. , Rao‐Bindal Krithi , Kleinerman Eugenie S. 
Published: February 2011

AbstractBACKGROUND:Patients with lung metastases from osteosarcoma (OS) have poor response to salvage therapy. Understanding the mechanisms involved in the metastatic process of OS may lead to new effective therapeutic approaches. The authors reported previously that up‐regulation of the Fas receptor by transfecting OS cells with Fas plasmid inhibited the in vivo growth of metastases in the lungs.METHODS:In the current study, the authors treated OS cells with the histone deacetylase inhibitor SNDX‐275 and studied its cytotoxicity and its effect on Fas signaling in vitro and in vivo.RESULTS:Subtoxic doses of SNDX‐275 were able to activate the Fas pathway in OS cells by increasing the expression of Fas messenger RNA; however, the increased expression was not always followed by increased levels of Fas receptor expression on the cell surface. The treatment of cells with a combination of SNDX‐275 and Fas ligand (FasL) had a stronger cytotoxic effect on tested OS cells than either agent alone. Inhibition of the Fas pathway in cells by inhibition of the Fas‐associated death domain (FADD) molecule eliminated this combination effect, indicating that activity of FADD is important for the efficacy of this agent in the FasL‐expressing environment of the lungs. Intranasal administration of SNDX‐275 in mice with OS lung metastases revealed that SNDX‐275 may inhibit metastatic growth at a dose of 0.13 mg/kg, which is approximately 200‐fold lower than the therapeutically effective oral dose reported previously.CONCLUSIONS:The current findings indicated that SNDX‐275 can activate Fas signaling in OS cells in vitro and in vivo and that the administration of SDNX‐275 by inhalation is feasible as a treatment for OS metastases and warrants its further investigation. Cancer 2011. © 2011 American Cancer Society.
A phase 2 study of temsirolimus (CCI‐779) in patients with soft tissue sarcomas
Authors: Okuno Scott , Bailey Howard , Mahoney Michelle R. , Adkins Douglas , Maples William , Fitch Tom , Ettinger David , Erlichman Charles , Sarkaria Jann N. 
Published: February 2011

AbstractBACKGROUND:The primary goal of this trial was to evaluate the confirmed response rate of temsirolimus (CCI‐779), a mammalian target of rapamycin in patients with advanced soft tissue sarcomas (STS).METHODS:Patients ≥18 years with measurable advanced STS, no prior chemotherapy for metastatic disease (adjuvant and neoadjuvant chemotherapy allowed), adequate organ function, and performance status of ≤2 were eligible. After premedication with an antihistamine, CCI‐779 was given intravenously at 25 mg over 30 minutes on Days 1, 8, 15, and 22, repeated every 4 weeks. The primary endpoint was confirmed response rate per Response Evaluation Criteria in Solid Tumors.RESULTS:Between June 2004 and November 2005, a total of 41 patients were enrolled and began treatment; 40 patients are evaluable for response and adverse events. The median age was 62 years (range, 28‐72 years) with 56% women. Eighty percent had high‐grade STS, and 22% had prior adjuvant chemotherapy. There were 2 patients (5%; 95% confidence interval [CI], 1‐17) (undifferentiated fibrosarcoma and uterine leiomyosarcoma) who achieved a confirmed partial response lasting 3 and 17 months, respectively. Thirty‐nine (95%) patients have progressed, with a median time to progression of 2.0 months (95% CI, 1.8‐3.5). The median overall survival was 7.6 months (95% CI, 6.1‐15.9). Forty‐three percent experienced grade 3+ adverse events that were possibly related to therapy.CONCLUSIONS:Temsirolimus in this patient population of STS had limited clinical activity and had moderate toxicities. Cancer 2011. © 2011 American Cancer Society.
Preservation of fine‐needle aspiration specimens for future use in RNA‐based molecular testing
Authors: Ladd Amy C. , O'Sullivan‐Mejia Emerald , Lea Tasha , Perry Jessica , Dumur Catherine I. , Dragoescu Ema , Garrett Carleton T. , Powers Celeste N. 
Published: February 2011

AbstractBACKGROUND:The application of ancillary molecular testing is becoming more important for the diagnosis and classification of disease. The use of fine‐needle aspiration (FNA) biopsy as the means of sampling tumors in conjunction with molecular testing could be a powerful combination. FNA is minimally invasive, cost effective, and usually demonstrates accuracy comparable to diagnoses based on excisional biopsies. Quality control (QC) and test validation requirements for development of molecular tests impose a need for access to pre‐existing clinical samples. Tissue banking of excisional biopsy specimens is frequently performed at large research institutions, but few have developed protocols for preservation of cytologic specimens. This study aimed to evaluate cryopreservation of FNA specimens as a method of maintaining cellular morphology and ribonucleic acid (RNA) integrity in banked tissues.METHODS:FNA specimens were obtained from fresh tumor resections, processed by using a cryopreservation protocol, and stored for up to 27 weeks. Upon retrieval, samples were made into slides for morphological evaluation, and RNA was extracted and assessed for integrity by using the Agilent Bioanalyzer (Agilent Technologies, Santa Clara, Calif).RESULTS:Cryopreserved specimens showed good cell morphology and, in many cases, yielded intact RNA. Cases showing moderate or severe RNA degradation could generally be associated with prolonged specimen handling or sampling of necrotic areas.CONCLUSIONS:FNA specimens can be stored in a manner that maintains cellular morphology and RNA integrity necessary for studies of gene expression. In addition to addressing quality control (QC) and test validation needs, cytology banks will be an invaluable resource for future molecular morphologic and diagnostic research studies. Cancer (Cancer Cytopathol) 2011;. © 2011 American Cancer Society.
Gossip‐based scalable directed diffusion for wireless sensor networks
Authors: Tang Junhua , Dai Sisi , Li Jianhua , Li Shenghong 
Published: February 2011

AbstractDirected diffusion (DD) is a promising data‐centric routing scheme for wireless sensor networks (WSNs). But the heavy flooding overhead involved in interest propagation causes scalability issues when DD is applied in large scale, interactive and dynamically changing sensor networks. To solve this problem, we propose a scalable version of DD called gossip‐based scalable directed diffusion (GSDD) in this paper. GSDD uses the same routing framework as DD but integrates gossiping in interest propagation and employs dynamic regional gossiping in path exploration phase to reduce the flooding overhead. Our analysis and simulation results demonstrate that GSDD is able to reduce the data delivery cost of DD by up to 25%; thus, significantly reduce energy consumption and prolong the lifetime of sensor nodes. Therefore, GSDD overcomes the scalability problem of DD and is suitable for large‐scale WSNs. Copyright © 2011 John Wiley & Sons, Ltd.
Neurogenin3 initiates stepwise delamination of differentiating endocrine cells during pancreas development
Authors: Gouzi Mathieu , Kim Yung Hae , Katsumoto Keiichi , Johansson Kerstin , Grapin‐Botton Anne 
Published: February 2011

AbstractDuring development, pancreatic endocrine cells are specified within the pancreatic epithelium. They subsequently delaminate out of the epithelium and cluster in the mesenchyme to form the islets of Langerhans. Neurogenin3 (Ngn3) is a transcription factor required for the differentiation of all endocrine cells and we investigated its role in their delamination. We observed in the mouse pancreas that most Ngn3‐positive cells have lost contact with the lumen of the epithelium, showing that the delamination from the progenitor layer is initiated in endocrine progenitors. Subsequently, in both mouse and chick newly born endocrine cells at the periphery of the epithelium strongly decrease E‐cadherin, break‐down the basal lamina and cluster into islets of Langerhans. Repression of E‐cadherin is sufficient to promote delamination from the epithelium. We further demonstrate that Ngn3 indirectly controls Snail2 protein expression post‐transcriptionally to repress E‐cadherin. In the chick embryo, Ngn3 independently controls epithelium delamination and differentiation programs. Developmental Dynamics 240:589–604, 2011. © 2011 Wiley‐Liss, Inc.