bluuebunny/crossref_metadata_2025_split huggingface dataset:
The expression of CXCR4, CXCL12 and CXCR7 in malignant pleural mesothelioma
Authors: Li Tong , Li Hui , Wang Yucheng , Harvard Chansonette , Tan Jia‐Li , Au Alfred , Xu Zhidong , Jablons David M , You Liang 
Published: November 2010

AbstractThe chemokine CXCL12 and its receptors, CXCR4 and CXCR7, are involved in tumour progression, metastasis, and survival. We investigated the expression of CXCR4, CXCL12, and CXCR7 in malignant pleural mesothelioma to determine if they are possible biomarkers and potential therapeutic targets. Forty‐one mesothelioma tumour tissues, ten normal human pleural tissues, and two mesothelioma cell lines were stained with anti‐CXCR4, anti‐CXCL12, anti‐CXCR7, and anti‐p‐Akt antibodies. RT‐PCR was performed to determine the expression of CXCR4, CXCL12, and CXCR7 in six human mesothelioma cell lines (H28, 211H, H2052, ms‐1, H290, and H513) and one human normal mesothelial cell line, LP9. These seven cell lines were also stained with anti‐CXCR7. We found that CXCR4 and CXCL12 were expressed in 97.6% and 78.0% mesothelioma tissue samples, concurrently with strong expression of p‐Akt (R2 = 0.739 and 0.620, respectively). In addition, CXCR7 expression was weaker than CXCR4 expression in mesothelioma tissues. Furthermore, RT‐PCR showed that CXCR4 and CXCL12 were overexpressed in 5/6 mesothelioma cell lines (211H, H2052, ms‐1, H290, and H513), whereas CXCR7 was overexpressed in only 2/6 (H513 and H2052). Moreover, we found that the CXCR4 antagonist AMD3100 inhibited the growth of all five mesothelioma cell lines that overexpress CXCR4 and CXCL12. Our results suggest that the Akt–mTOR pathway is involved during the interruption of the CXCL12/CXCR4 axis in these five mesothelioma cell lines. In conclusion, CXCR4 and CXCL12 are highly expressed in most mesothelioma cell lines and tumour tissues, suggesting that CXCR4 and CXCL12 may be used as biomarkers for patients with mesothelioma. The CXCL12–CXCR4 interaction may be a potential therapeutic target for mesothelioma. Copyright © 2011 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
The Protein Information and Property Explorer 2: Gaggle‐like exploration of biological proteomic data within one webpage
Authors: Ramos Hector , Shannon Paul , Brusniak Mi‐Youn , Kusebauch Ulrike , Moritz Robert L. , Aebersold Ruedi 
Published: November 2010

AbstractThe Protein Information and Property Explorer 2 (PIPE2) is an enhanced software program and updated web application that aims at providing the proteomic researcher a simple, intuitive user interface through which to begin inquiry into the biological significance of a list of proteins typically produced by MS/MS proteomic processing software. PIPE2 includes an improved interface, new data visualization options, and new data analysis methods for combining disparate, but related, data sets. In particular, PIPE2 has been enhanced to handle multi‐dimensional data such as protein abundance, gene expression, and/or interaction data. The current architecture of PIPE2, modeled after that of Gaggle (a programming infrastructure for interoperability between separately developed software tools), contains independent functional units that can be instantiated and pieced together at the user's discretion to form a pipelined analysis workflow. Among these functional units is the Network Viewer component, which adds rich network analysis capabilities to the suite of existing proteomic web resources. Additionally, PIPE2 implements a framework within which new analysis procedures can be easily deployed and distributed over the World Wide Web. PIPE2 is available as a web service at http://pipe2.systemsbiology.net/.
Protective Effects of Berberine in an Experimental Rat Osteoarthritis Model
Authors: Hu Peng‐fei , Chen Wei‐ping , Tang Jing‐li , Bao Jia‐peng , Wu Li‐dong 
Published: November 2010

AbstractBerberine shows anticancer, antibacterial, antiinflammatory and antioxidant effects and may be useful in many clinical applications. The effects of berberine on articular cartilage metabolism remain unknown, so this study was performed to evaluate these effects in vitro and in vivo. For the in vitro work, rat articular chondrocytes were cultured in a monolayer and matrix metalloproteinase‐1 (MMP‐1), ‐3, ‐13 and tissue inhibitor of metalloproteinase (TIMP‐1) expression was evaluated by real‐time quantitative PCR. Nitric oxide (NO) levels were determined using the Griess reaction, and glycosaminoglycan (GAG) release was measured using the dimethylmethylene blue method. For the in vivo work, berberine was administered by intraarticular injection, and the effects on MMPs and TIMP‐1 were examined at the gene and protein levels. Berberine was found to inhibit the expression of MMP‐1, ‐3 and ‐13, and increased the level of TIMP‐1 at the mRNA level in a dose‐dependent manner. In IL‐1β‐induced rat articular chondrocytes, berberine decreased IL‐1β‐induced GAG release and NO production. Meanwhile, high‐dose berberine exhibited an anticatabolic effect in an IL‐1β‐induced rat osteoarthritis (OA) model. These findings suggest that berberine may play a protective role in the development of OA and may be useful in the treatment of OA. Copyright © 2010 John Wiley & Sons, Ltd.
Effect of relative humidity on energy absorption properties of honeycomb paperboards
Authors: E Yu‐Ping , Wang Zhi‐Wei 
Published: November 2010

AbstractThe effect of relative humidity (RH) on the energy absorption characteristics of honeycomb paperboard is investigated experimentally. Results indicate that RH has no significant effect on plateau stress, or energy absorption capacity of honeycomb paperboard in RH range between 40–75%, but its energy absorption properties decrease substantially with the increasing of RH when the RH exceeds 75%. It is concluded that RH is a significant consideration for honeycomb paperboard as a cushioning material used in extremely humid environments most probably encountered in south‐east coast of China, while under ordinary circumstances, there is no need to consider the effect of RH. Energy absorption diagram based on the effect of RH is constructed from the stress‐strain curves obtained from tests, and its application for a given energy absorption task is exemplified. The model established in this paper could be used for practical application for the designing optimization and material selection of packaging products in actual logistics environments. Copyright © 2010 John Wiley & Sons, Ltd.
Regularized implicit surface reconstruction from points and normals
Authors: Mederos B. , Lage M. , Arouca S. , Petronetto F. , Velho L. , Lewiner T. , Lopes H. 
Published: November 2010

AbstractWe consider the problem of surface reconstruction of a geometric object from a finite set of sample points with normals. Our contribution is to present a new scheme for implicit surface reconstruction. Similarly to the multilevel partition of unity (MPU) method we hierarchically divide the domain obtaining local approximation for the object on each part, and then patch all together obtaining a global description of the object. Our new scheme uses ridge regression and weighted gradient one fitting techniques to get better stability on local approximations. The method behaves reasonably on sparse set of points and data with holes as those which comes from 3D scanning of real objects.
Synthesis of N‐(3‐Oxapropanoxyl)dodecanamide and its Application in Surfactant‐Polymer Flooding
Authors: Cui Zheng‐gang , Song Hong‐xing , Yu Jun‐jie , Jiang Jian‐zhong , Wang Feng 
Published: November 2010

AbstractAlkali‐surfactant‐polymer (ASP) flooding has been considered to be one of the useful tertiary oil recovery techniques. However, field tests in China have revealed that serious side effects may occur due to using alkali. Thus alkali‐free SP flooding is more favorable in China. Unfortunately, surfactants effective in ASP flooding are usually ineffective in the absence of alkali and new surfactants need to be designed. In this paper N‐(3‐Oxapropanoxyl)dodecanamide as a pure compound and a mixture of homologues with narrow EO number distribution, synthesized by the reaction of lauroyl chloride with diglycolamine and addition of one ethylene oxide to coconut monoethanolamide respectively, are examined for their adaptability in SP flooding. It is found that, when mixed with betaines, both products can reduce Daqing crude oil/connate water interfacial tension to a magnitude of 10−3–10−4 mNm−1 at 45 °C in a wide surfactant concentration range, 0.01–0.5 wt%, and oil displacement tests using natural cores indicates that a tertiary oil recovery of 18.6 ± 0.4% OOIP can be achieved by SP flooding with the N‐(3‐Oxapropanoxyl)dodecanamide as the main surfactant without adding any alkaline agent and neutral electrolyte. N‐(3‐Oxapropanoxyl)dodecanamide, as a nonionic surfactant without a cloud point and producible industrially from renewable materials, is an ideal surfactant for SP flooding in the absence of an alkaline agent.
IL‐1beta in the trigeminal subnucleus caudalis contributes to extra‐territorial allodynia/hyperalgesia following a trigeminal nerve injury
Authors: Takahashil Kouji , Watanabel Mineo , Suekawal Yohei , Itol Goshi , Inubushil Toshihiro , Hirosel Naoto , Murasakil Kyoko , Hiyamal Shinji , Uchidal Takashi , Tannel Kazuo 
Published: November 2010

AbstractIt has been reported that the whisker pad (WP) area, which is innervated by the second branch of the trigeminal nerve, shows allodynia/hyperalgesia following transection of the mental nerve (MN: the third branch of the trigeminal nerve). However, the mechanisms of this extra‐territorial pain induction still remain unclear. Glia and cytokines are known to facilitate perception of noxious input, raising a possibility that these non‐neuronal elements are involved in the induction and spread of allodynia/hyperalgesia at non‐injured skin territory. One day after MN transection, tactile allodynia/hyperalgesia developed on the ipsilateral WP area, which is in the non‐injured skin territory. The tactile allodynia/hyperalgesia lasted for more than 56 days. In response to MN transection, astrocytes and microglia appeared to be in an activated state, and interleukin (IL)‐1beta was up‐regulated in astrocytes in the trigeminal subnucleus caudalis (Vc). Allodynia/hyperalgesia at WP area induced by MN transection was attenuated dose‐dependently by IL‐1 receptor antagonist IL‐1ra (i.t., 0.05, 0.5, and 5pg/rat). Fos‐like immunoreactive (Fos‐Li) neurons were observed in the Vc after non‐noxious mechanical stimulation of the WP area in the rats with MN transection. Administration of IL‐1ra also attenuated the number of Fos‐Li neurons dose‐dependently. Administration of a noncompetitive antagonist of NMDA receptors MK‐801 (i.t., 5μg/rat) reversed allodynia/hyperalgesia. IL‐1 receptor type I (IL‐1RI) was localized in Fos‐ and phospho NR1‐immunoreactive neurons. These results suggest that IL‐1beta in the Vc plays an important role in the development of extra‐territorial tactile allodynia/hyperalgesia after MN transection.
The somatostatin analogue octreotide inhibits capsaicin‐mediated activation of nociceptive primary afferent fibres in spinal cord lamina II (substantia gelatinosa)
Authors: Bencivinnil Ileana , Ferrinil Francesco , Saliol Chiara , Beltramol Massimiliano , Merighil Adalberto 
Published: November 2010

AbstractSomatostatin (SST) in spinal cord has been linked with the inhibition of nociceptive neurotransmission in several experimental paradigms. The SST2 receptor (SSTR2) is the main SST receptor subtype in the superficial dorsal horn (DH) and is activated, besides to the naïve peptide, by the SST synthetic analogue octreotide (OCT). In the present work, we have studied the central effects of SSTR2 activation on capsaicin (CAP)‐induced glutamate release in mouse DH.In neurons of the lamina II of DH, CAP (2μM) induced a strong increase of mEPSC frequency that was significantly reduced (70%) by OCT. SSTR2 involvement was assessed by using the specific antagonist CYN 154806. No differences were observed between frequency increase in CAP alone vs. CAP in the presence of CYN 154806+OCT. The effect of OCT was further investigated by studyingc‐fosexpression in spinal cord slices. The CAP‐induced increase in density of Fos immunoreactive nuclei in the superficial DH was strongly prevented by OCT.SSTR2a (a splicing variant of SSTR2) immunoreactivity was found in both pre‐ and post‐synaptic compartments of laminae I—II synapses. By light and electron microscopy, SSTR2a was mainly localized onto non‐peptidergic isolectin B4 (IB4)‐positive primary afferent fibres (PAFs). A subset of them was also found to express the CAP receptor TRPV1.These data show that the SST analogue OCT inhibits CAP‐mediated activation of non‐peptidergic nociceptive PAFs in lamina II. Our data indicate that SSTR2a plays an important role in the pre‐synaptic modulation of central excitatory nociceptive transmission in mouse.
A randomized clinical trial of a levonorgestrel‐releasing intrauterine system and a low‐dose combined oral contraceptive for fibroid‐related menorrhagia
Authors: Sayed Gamal H. , Zakherah Mahmoud S. , El‐Nashar Sherif A. , Shaaban Mamdouh M. 
Published: November 2010

AbstractObjectiveTo compare the efficacy of a levonorgestrel‐releasing intrauterine system (LNG‐IUS) with that of a low‐dose combined oral contraceptive (COC) in reducing fibroid‐related menorrhagia.MethodsIn this single‐center, open, randomized clinical trial, 58 women with menorrhagia who desired contraception were randomized to receive a LNG‐IUS or COC. The outcomes included treatment failure, defined as the need for another treatment; menstrual blood loss (MBL) by the alkaline hematin method and a pictorial assessment chart (PBAC); hemoglobin levels; and “lost days.”ResultsTreatment failed in 6 women (23.1%) in the LNG‐IUS group and 11 (37.9%) in the COC group, for a hazard ratio of 0.46 (95% CI, 0.17–1.17, P = 0.101). Using the alkaline hematin test, the reduction of MBL was significantly greater in the LNG‐IUS group (90.9% ± 12.8% vs 13.4% ± 11.1%; P < 0.001). Using PBAC scores, the reduction was also significantly greater in the LNG‐IUS group (88.0% ± 16.5% vs 53.5% ± 51.2%; P = 0.02). Moreover, hemoglobin levels increased from 9.7 ± 1.9 g/dL to 11.7 ± 1.2 g/dL (P < 0.001) and lost days decreased from 8.2 ± 3.3 days to 1.3 ± 1.5 days (P = 0.003) in the LNG‐IUS group.ConclusionAlthough the rate of treatment failure was similar in both groups, the LNG‐IUS was more effective in reducing MBL than the COC in women with fibroid‐related menorrhagia.
Dietary pattern and weight change in a 5-year follow-up among Chinese adults: results from the Jiangsu Nutrition Study
Authors: Shi Zumin , Yuan Baojun , Hu Gang , Dai Yue , Zuo Hui , Holmboe-Ottesen Gerd 
Published: November 2010

The aim of the present study was to examine the association between dietary patterns and weight change prospectively. We followed up 1231 adults aged 20 years and older (mean age 49·3 years) from 2002 to 2007. Food intake was assessed at baseline by an FFQ. Factor analysis was used to identify dietary patterns. Nutrient intake was measured by food weighing plus consecutive individual 3 d food records. Body weight and height were measured. The mean 5-year weight gain among all participants was 0·8 (sd4·7) kg. The mean weight changes across quartiles of the ‘traditional’ pattern (rice, fresh vegetables, pork and wheat flour) were 2·0, 0·6, 0·6 and 0·0 kg. The corresponding values were 0·4, 0·5, 0·7 and 1·6 kg across quartiles of the ‘vegetable-rich’ pattern. No significant association between the ‘macho’ dietary pattern (meat and alcohol), the ‘sweet tooth’ pattern (drinks and cake) and weight gain was found. In multivariate linear regression, using the first quartile as the reference, participants in the highest quartile of the ‘traditional’ pattern had a β of − 2·18 (95 % CI − 2·91, − 1·45) for weight gain. However, the β from weight change of the highest quartile of the ‘vegetable-rich’ pattern was 1·00 (95 % CI 0·25, 1·74). In conclusion, the ‘traditional’ dietary pattern was negatively and the ‘vegetable-rich’ pattern was positively associated with weight gain in Chinese adults.
Colonization of subsurface microbial observatories deployed in young ocean crust
Authors: Orcutt Beth N , Bach Wolfgang , Becker Keir , Fisher Andrew T , Hentscher Michael , Toner Brandy M , Wheat C Geoffrey , Edwards Katrina J 
Published: November 2010

Abstract
Oceanic crust comprises the largest hydrogeologic reservoir on Earth, containing fluids in thermodynamic disequilibrium with the basaltic crust. Little is known about microbial ecosystems that inhabit this vast realm and exploit chemically favorable conditions for metabolic activities. Crustal samples recovered from ocean drilling operations are often compromised for microbiological assays, hampering efforts to resolve the extent and functioning of a subsurface biosphere. We report results from the first in situ experimental observatory systems that have been used to study subseafloor life. Experiments deployed for 4 years in young (3.5 Ma) basaltic crust on the eastern flank of the Juan de Fuca Ridge record a dynamic, post-drilling response of crustal microbial ecosystems to changing physical and chemical conditions. Twisted stalks exhibiting a biogenic iron oxyhydroxide signature coated the surface of mineral substrates in the observatories; these are biosignatures indicating colonization by iron oxidizing bacteria during an initial phase of cool, oxic, iron-rich conditions following observatory installation. Following thermal and chemical recovery to warmer, reducing conditions, the in situ microbial structure in the observatory shifted, becoming representative of natural conditions in regional crustal fluids. Firmicutes, metabolic potential of which is unknown but may involve N or S cycling, dominated the post-rebound bacterial community. The archaeal community exhibited an extremely low diversity. Our experiment documented in situ conditions within a natural hydrological system that can pervade over millennia, exemplifying the power of observatory experiments for exploring the subsurface basaltic biosphere, the largest but most poorly understood biotope on Earth.
Developmental Sexual Dimorphism of the Oral and Pharyngeal Portions of the Vocal Tract: An Imaging Study
Authors: Vorperian Houri K. , Wang Shubing , Schimek E. Michael , Durtschi Reid B. , Kent Ray D. , Gentry Lindell R. , Chung Moo K. 
Published: November 2010

PurposeThe anatomic origin for prepubertal vowel acoustic differences between male and female subjects remains unknown. The purpose of this study is to examine developmental sex differences in vocal tract (VT) length and its oral and pharyngeal portions.MethodNine VT variables were measured from 605 imaging studies (magnetic resonance imaging and computed tomography) of subjects between birth and age 19 years. Given sex differences in growth rate (Vorperian et al., 2009), assessment of sex differences was done through use of a localized comparison window of 60 months. Analysis entailed applying this comparison window first to 4 discrete age cohorts, followed by a progressive assessment in which this comparison window was moved in 1-month increments from birth across all ages.ResultsFindings document significant postpubertal sex differences in both the oral and pharyngeal portions of the VT. They also document periods of significant prepubertal sex differences in the oral region first, followed by segments in the pharyngeal region.ConclusionsAssessment of developmental sex differences using localized age ranges is effective in unveiling sex differences that growth rate differences may conceal. Findings on the presence of prepubertal sex differences in the oral region of the VT may clarify, in part, the anatomic basis of documented prepubertal acoustic differences.
Modulation of Neck Intermuscular Beta Coherence During Voice and Speech Production
Authors: Stepp Cara E. , Hillman Robert E. , Heaton James T. 
Published: November 2010

Purpose
The purpose of this study was to better understand neck intermuscular beta coherence (15–35 Hz; NIBcoh) in healthy individuals, with respect to modulation by behavioral tasks.

Method
Mean NIBcoh was measured using surface electromyography at 2 anterior neck locations in 10 individuals during normal speech, static nonspeech maneuvers, “clear” speech (intentionally produced to maximize intelligibility), divided-attention speech, singing, and mimicked hyperfunctional speech.

Results

An analysis of variance showed significant effects of both individual and condition (
p
= .001) on the mean beta-band intermuscular coherence. Dunnett’s simultaneous paired
t
tests found decreased NIBcoh during low-attention speech, singing, and hyperfunctional speech (
p
adj
< .05), but no significant difference in NIBcoh during nonspeech tasks or clear speech production relative to normal speech.

Conclusions
Compared with normal speech, mean NIBcoh was decreased in a divided-attention speech task, but clear speech did not result in increased mean coherence relative to normal speech, possibly due to ceiling effects caused by heightened attention and precision during experimental recording. Mimicking a strained, hyperfunctional voice resulted in a reduction in mean beta intermuscular coherence quantitatively and qualitatively similar to the lowered values of mean beta coherence seen in individuals with vocal nodules relative to individuals with normal voice.
Energetics and mechanisms of folding and flipping the myristoyl switch in the β-trefoil protein, hisactophilin
Authors: Smith Martin T. J. , Meissner Joseph , Esmonde Samantha , Wong Hannah J. , Meiering Elizabeth M. 
Published: November 2010

Myristoylation, the covalent linkage of a saturated, C
14
fatty acyl chain to the N-terminal glycine in a protein, plays a vital role in reversible membrane binding and signaling by the modified proteins. Currently, little is known about the effects of myristoylation on protein folding and stability, or about the energetics and molecular mechanisms of switching involving states with sequestered versus accessible myristoyl group. Our analysis of these effects in hisactophilin, a histidine-rich protein that binds cell membranes and actin in a pH-dependent manner, shows that myristoylation significantly increases hisactophilin stability, while also markedly increasing global protein folding and unfolding rates. The switching between sequestered and accessible states is pH dependent, with an apparent pK
switch
of 6.95, and an apparent free energy change of 2.0 kcal·mol
-1
. The myristoyl switch is linked to the reversible uptake of ∼1.5 protons, likely by histidine residues. This pH dependence of switching appears to be the physical basis of the sensitive, pH-dependent regulation of membrane binding observed in vivo. We conclude that an increase in protein stability upon modification and burial of the attached group is likely to occur in numerous proteins modified with fatty acyl or other hydrophobic groups, and that the biophysical effects of such modification are likely to play an important role in their functional switches. In addition, the increased global dynamics caused by myristoylation of hisactophilin reveals a general mechanism whereby hydrophobic moieties can make nonnative interactions or relieve strain in transition states, thereby increasing the rates of interconversion between different states.