bluuebunny/crossref_metadata_2025_split huggingface dataset:
Mitochondrial biogenesis in cold-bodied fishes
Authors: O'Brien Kristin M. 
Published: December 2010

SummaryMitochondrial biogenesis is induced in response to cold temperature in many organisms. The effect is particularly pronounced in ectotherms such as fishes, where acclimation to cold temperature increases mitochondrial density. Some polar fishes also have exceptionally high densities of mitochondria. The net effect of increasing mitochondrial density is threefold. First, it increases the concentration of aerobic metabolic enzymes per gram of tissue, maintaining ATP production. Second, it elevates the density of mitochondrial membrane phospholipids, enhancing rates of intracellular oxygen diffusion. Third, it reduces the diffusion distance for oxygen and metabolites between capillaries and mitochondria. Although cold-induced mitochondrial biogenesis has been well documented in fishes, little is known about the molecular pathway governing it. In mammals, the co-transcriptional activator peroxisome proliferator-activated receptor gamma coactivator 1 alpha (PGC-1α) is thought to coordinate the three components of mitochondrial biogenesis: the synthesis of mitochondrial proteins, the synthesis of phospholipids and the replication of mitochondrial DNA. Some components of the mitochondrial biogenic pathway are conserved between fishes and mammals, yet the pathway appears more versatile in fishes. In some tissues of cold-acclimated fishes, the synthesis of mitochondrial proteins increases in the absence of an increase in phospholipids, whereas in some polar fishes, densities of mitochondrial phospholipids increase in the absence of an increase in proteins. The ability of cold-bodied fishes to fine-tune the mitochondrial biogenic pathway may allow them to modify mitochondrial characteristics to meet the specific needs of the cell, whether it is to increase ATP production or enhance oxygen diffusion.
The sugar oxidation cascade: aerial refueling in hummingbirds and nectar bats
Authors: Suarez Raul K. , M. L. Gerardo Herrera , Welch Kenneth C. 
Published: December 2010

SummaryMost hummingbirds and some species of nectar bats hover while feeding on floral nectar. While doing so, they achieve some of the highest mass-specific values among vertebrates. This is made possible by enhanced functional capacities of various elements of the ‘O2 transport cascade’, the pathway of O2 from the external environment to muscle mitochondria. Fasted hummingbirds and nectar bats fly with respiratory quotients (RQs; ) of ∼0.7, indicating that fat fuels flight in the fasted state. During repeated hover-feeding on dietary sugar, RQ values progressively climb to ∼1.0, indicating a shift from fat to carbohydrate oxidation. Stable carbon isotope experiments reveal that recently ingested sugar directly fuels ∼80 and 95% of energy metabolism in hover-feeding nectar bats and hummingbirds, respectively. We name the pathway of carbon flux from flowers, through digestive and cardiovascular systems, muscle membranes and into mitochondria the ‘sugar oxidation cascade’. O2 and sugar oxidation cascades operate in parallel and converge in muscle mitochondria. Foraging behavior that favours the oxidation of dietary sugar avoids the inefficiency of synthesizing fat from sugar and breaking down fat to fuel foraging. Sugar oxidation yields a higher P/O ratio (ATP made per O atom consumed) than fat oxidation, thus requiring lower hovering per unit mass. We propose that dietary sugar is a premium fuel for flight in nectarivorous, flying animals.
Physiological, behavioral and biochemical adaptations of intertidal fishes to hypoxia
Authors: Richards Jeffrey G. 
Published: December 2010

SummaryHypoxia survival in fish requires a well-coordinated response to either secure more O2 from the hypoxic environment or to limit the metabolic consequences of an O2 restriction at the mitochondria. Although there is a considerable amount of information available on the physiological, behavioral, biochemical and molecular responses of fish to hypoxia, very little research has attempted to determine the adaptive value of these responses. This article will review current attempts to use the phylogenetically corrected comparative method to define physiological and behavioral adaptations to hypoxia in intertidal fish and further identify putatively adaptive biochemical traits that should be investigated in the future. In a group of marine fishes known as sculpins, from the family Cottidae, variation in hypoxia tolerance, measured as a critical O2 tension (Pcrit), is primarily explained by variation in mass-specific gill surface area, red blood cell hemoglobin–O2 binding affinity, and to a lesser extent variation in routine O2 consumption rate (). The most hypoxia-tolerant sculpins consistently show aquatic surface respiration (ASR) and aerial emergence behavior during hypoxia exposure, but no phylogenetically independent relationship has been found between the thresholds for initiating these behaviors and Pcrit. At O2 levels below Pcrit, hypoxia survival requires a rapid reorganization of cellular metabolism to suppress ATP consumption to match the limited capacity for O2-independent ATP production. Thus, it is reasonable to speculate that the degree of metabolic rate suppression and the quantity of stored fermentable fuel is strongly selected for in hypoxia-tolerant fishes; however, these assertions have not been tested in a phylogenetic comparative model.
The biological control of voluntary exercise, spontaneous physical activity and daily energy expenditure in relation to obesity: human and rodent perspectives
Authors: Garland Theodore , Schutz Heidi , Chappell Mark A. , Keeney Brooke K. , Meek Thomas H. , Copes Lynn E. , Acosta Wendy , Drenowatz Clemens , Maciel Robert C. , van Dijk Gertjan , Kotz Catherine M. , Eisenmann Joey C. 
Published: December 2010

SummaryMammals expend energy in many ways, including basic cellular maintenance and repair, digestion, thermoregulation, locomotion, growth and reproduction. These processes can vary tremendously among species and individuals, potentially leading to large variation in daily energy expenditure (DEE). Locomotor energy costs can be substantial for large-bodied species and those with high-activity lifestyles. For humans in industrialized societies, locomotion necessary for daily activities is often relatively low, so it has been presumed that activity energy expenditure and DEE are lower than in our ancestors. Whether this is true and has contributed to a rise in obesity is controversial. In humans, much attention has centered on spontaneous physical activity (SPA) or non-exercise activity thermogenesis (NEAT), the latter sometimes defined so broadly as to include all energy expended due to activity, exclusive of volitional exercise. Given that most people in Western societies engage in little voluntary exercise, increasing NEAT may be an effective way to maintain DEE and combat overweight and obesity. One way to promote NEAT is to decrease the amount of time spent on sedentary behaviours (e.g. watching television). The effects of voluntary exercise on other components of physical activity are highly variable in humans, partly as a function of age, and have rarely been studied in rodents. However, most rodent studies indicate that food consumption increases in the presence of wheels; therefore, other aspects of physical activity are not reduced enough to compensate for the energetic cost of wheel running. Most rodent studies also show negative effects of wheel access on body fat, especially in males. Sedentary behaviours per se have not been studied in rodents in relation to obesity. Several lines of evidence demonstrate the important role of dopamine, in addition to other neural signaling networks (e.g. the endocannabinoid system), in the control of voluntary exercise. A largely separate literature points to a key role for orexins in SPA and NEAT. Brain reward centers are involved in both types of physical activities and eating behaviours, likely leading to complex interactions. Moreover, voluntary exercise and, possibly, eating can be addictive. A growing body of research considers the relationships between personality traits and physical activity, appetite, obesity and other aspects of physical and mental health. Future studies should explore the neurobiology, endocrinology and genetics of physical activity and sedentary behaviour by examining key brain areas, neurotransmitters and hormones involved in motivation, reward and/or the regulation of energy balance.
The metabolic demands of endosymbiotic chemoautotrophic metabolism on host physiological capacities
Authors: Childress J. J. , Girguis P. R. 
Published: December 2010

SummaryWhile chemoautotrophic endosymbioses of hydrothermal vents and other reducing environments have been well studied, little attention has been paid to the magnitude of the metabolic demands placed upon the host by symbiont metabolism and the adaptations necessary to meet such demands. Here we make the first attempt at such an evaluation, and show that moderate to high rates of chemoautotrophic or methanotrophic metabolism impose oxygen uptake and proton equivalent elimination demands upon the hosts that are much higher than is typical for the non-symbiotic annelid, bivalve and gastropod lineages to which they are related. The properties of the hosts are described and compared to determine which properties are associated with and predictive of the highest rates. We suggest that the high oxygen demand of these symbionts is perhaps the most limiting flux for the symbioses. Among the consequences of such demands has been the widespread presence of circulating and/or tissue hemoglobins in these symbioses that are necessary to support high metabolic rates in thioautotrophic endosymbioses. We also compare photoautotrophic with chemoautotrophic and methanotrophic endosymbioses to evaluate the differences and similarities in physiologies. These analyses suggest that the high demand for oxygen by chemoautotrophic and methanotrophic symbionts is likely a major factor precluding their endosymbiosis with cnidarians.
Nonshivering thermogenesis and its adequate measurement in metabolic studies
Authors: Cannon Barbara , Nedergaard Jan 
Published: December 2010

Summary
Alterations in nonshivering thermogenesis are presently discussed as being both potentially causative of and able to counteract obesity. However, the necessity for mammals to defend their body temperature means that the ambient temperature profoundly affects the outcome and interpretation of metabolic experiments. An adequate understanding and assessment of nonshivering thermogenesis is therefore paramount for metabolic studies. Classical nonshivering thermogenesis is facultative, i.e. it is only activated when an animal acutely requires extra heat (switched on in minutes), and adaptive, i.e. it takes weeks for an increase in capacity to develop. Nonshivering thermogenesis is fully due to brown adipose tissue activity; adaptation corresponds to the recruitment of this tissue. Diet-induced thermogenesis is probably also facultative and adaptive and due to brown adipose tissue activity. Although all mammals respond to injected/infused norepinephrine (noradrenaline) with an increase in metabolism, in non-adapted mammals this increase mainly represents the response of organs not involved in nonshivering thermogenesis; only the increase after adaptation represents nonshivering thermogenesis. Thermogenesis (metabolism) should be expressed per animal, and not per body mass [not even to any power (0.75 or 0.66)]. A ‘cold tolerance test’ does not examine nonshivering thermogenesis capacity; rather it tests shivering capacity and endurance. For mice, normal animal house temperatures are markedly below thermoneutrality, and the mice therefore have a metabolic rate and food consumption about 1.5 times higher than their intrinsic requirements. Housing and examining mice at normal house temperatures carries a high risk of identifying false positives for intrinsic metabolic changes; in particular, mutations/treatments that affect the animal's insulation (fur, skin) may lead to such problems. Correspondingly, true alterations in intrinsic metabolic rate remain undetected when metabolism is examined at temperatures below thermoneutrality. Thus, experiments with animals kept and examined at thermoneutrality are likely to yield an improved possibility of identifying agents and genes important for human energy balance.
Coma After Overdose with Duloxetine
Authors: Kruithof Maartje K , Bruins Nynke A , van Roon Eric N 
Published: December 2010

Objective To describe a case of a patient who became comatose after taking an overdose of duloxetine, a serotonin-norepinephrine reuptake inhibitor. Case Summary: A 49-year-old male ingested an overdose of duloxetine approximately 2 hours before presentation to the emergency department. On arrival he was drowsy, but easily awakened and oriented, with Glasgow Coma Score 14 (eyes 3, motor 6, verbal 5). immediately after admission, charcoal and magnesium sulfate were given to prevent further systemic absorption of medication through the gastrointestinal tract. No gastric lavage was pertormed. Six hours after drug intake the patient became unconscious (Glasgow Coma Score 7, eyes 2, motor 4, verbal 1). Full toxicologic screening showed a toxic duloxetine plasma concentration of 0.86 mg/L. The patient was admitted to the intensive care unit (ICU) and, on arrival, urinary retention was noted. During ICU admission the patient remained hemodynamically stable; approximately 12 hours after ingestion of duloxetine, he regained consciousness. Over the next 3 days the urinary output decreased to 60 mL/day. After 4 days patient was discharged without any remaining symptoms. Based on repeated plasma duloxetine serum concentration determinations, a plasma half-life of duloxetine was calculated to be 18 hours (reference range 9-19). Discussion: The Naranjo probability scale suggested that duloxetine was the probable cause for the symptoms described. Conclusion: Overdose with duloxetine can induce coma several hours after intake, with a last reversal in our case.
Is Nigerian Pidgin English English?
Authors: Akande Akinmade T. 
Published: December 2010

Abstract
The major aim of this paper is to demonstrate through the grammar of the verb phrase in Standard English (StdE) and Nigerian Pidgin English (NPE) that NPE is a distinct language. The paper draws on data collected from 30 Nigerian University Graduates from three ethnolinguistic regions. Although many scholars have pointed out that NPE is a language (Agheyisi, West African Pidgin: Simplification and Simplicity, University of Stanford, 1971; Elugbe and Omamor, Nigerian Pidgins: Background and Prospects, Heinemann, 1991), not many of them have examined the verb phrase in NPE with a view to showing that its grammar is distinctive. It has been shown in this paper that the NPE verb phrase is sharply different from StdE verb phrase, and because the verb is at the centre of the clause and can determine its argument, it can be argued that NPE whose verbal grammar is radically different from that of English is a separate language. The pattern of clustering of NPE verb phrases with other NPE verb phrases or other crieterial features of NPE is also a demonstration that NPE is a distinct language. Finally, the subjects made sharp switches from StdE to NPE, speaking in blocks of first one code and then the other: this is like the behaviour of bilinguals moving from one language to another.
Mots et expressions qualifiant le mélange des langues en picard et en flamand de France
Authors: Carton Fernand 
Published: December 2010

Abstract
In this paper I have attempted to classify and explain 30 words and phrases representative of language medley in Northern France. They illustrate a mixture of Germanic and Romance languages: Flemish in French and Picard, French in Flemish, Picard in French and vice versa. I will discuss imagination, word-play and lexical creativity in code-switching situations, as well as the dynamics of regional variation in the epilinguistic data under consideration.
Quantification and Statistical Analysis of Structural Similarities in Dialectological Area-Class Maps
Authors: Rumpf Jonas , Pickl Simon , Elspaß Stephan , König Werner , Schmidt Volker 
Published: December 2010

AbstractDialect atlases comprise considerable numbers of linguistic feature maps, i.e. dialect maps representing one linguistic feature each. Large amounts of data like these are often difficult to handle. This article presents a new quantitative method for the automatic analysis of such large corpora of linguistic feature maps. It makes use of geographical similarities between single maps to establish a system of criteria for structural relatedness. Furthermore, it employs statistical techniques to test whether given linguistic relations between the maps coincide significantly with structural relations. To achieve this, each underlying point-symbol map is converted into an area-class map (with all the original information still available). These area-class maps yield additional information regarding their structural composition. Cluster analysis is then employed to obtain groupings of similar maps. Such groupings facilitate the search for language-internal factors that influence the geographical distribution of linguistic variants, as the relevance of any given linguistic parameter for spatial patterns can be tested using statistical methods. Moreover, language-external factors, such as topographical conditions, can be tested in the same way. Thus, this new method allows for a profound and substantiated investigation of the regularities that can be found in the geographical distributions of linguistic variants.
Applying Quantitative Analysis Techniques to La flexió verbal en els dialectes catalans
Authors: Perea Maria-Pilar , Ueda Hiroto 
Published: December 2010

Abstract
The Majorcan dialectologist Antoni M. Alcover (1862–1932) was the first researcher to record information for use in the study of the conjugation of Catalan verbs. He compiled almost 500,000 verb forms corresponding to the complete conjugation of 75 verbs in 149 localities in the Catalan-speaking area. These data were published under the title “La flexió verbal en els dialectes catalans”. From 1999, work began to complete and computerize these materials in order to make them accessible to scholars in the field and to the public. These computer applications have a descriptive character: they show dialectal and subdialectal areas, and isoglosses, in a graphic form. However, it is also possible to use these materials from an interpretative point of view.
The aim of this paper is to apply a suitable dialectometric analysis to the “Verbal flexion” materials, which can only be interpreted using quantitative analysis techniques. Taking multiple answers into account, we use the calculation of the Mean Degree of Confluence and we compare the results with the dialectometric analysis obtained applying Hans Goebl's methodology, which is based on the use of single answers.
CDKN1B V109G polymorphism a new prognostic factor in sporadic medullary thyroid carcinoma
Authors: Pasquali Daniela , Circelli Luisa , Faggiano Antongiulio , Pancione Massimo , Renzullo Andrea , Elisei Rossella , Romei Cristina , Accardo Giacomo , Coppola Viviana Raffaella , De Palma Maurizio , Ferolla Piero , Grimaldi Franco , Colao Annamaria , Colantuoni Vittorio 
Published: December 2010

ContextCDKN1B encodes the cyclin-dependent kinase inhibitor p27Kip1 and is mutated in multiple endocrine neoplasia-like syndromes. CDKN1B also harbors single nucleotide polymorphisms; the T/G transversion at nucleotide 326 (the V109G variant) has been reported to be protective in breast, hereditary prostate, and pancreatic tumors. Association of CDNK1B mutations or polymorphisms with sporadic medullary thyroid carcinoma (MTC) has not been investigated yet.Objective and designWe screened germline DNA from 84 patients affected by sporadic MTC and 90 healthy age- and gender-matched controls for CDKN1B mutations or polymorphisms by PCR amplification and sequencing of the amplicons. We also tested all germline and 50 tumor tissue DNA for RET proto-oncogene mutations. Computed tomography, ultrasound scans, and serum calcitonin were carried out before surgery and during the follow-up and associated with CDKN1B polymorphism and disease remission.ResultsThe T/G transversion at nucleotide 326 was the only DNA variation detected. The overall frequency of the T/G and G/G alleles in combination was 46.4%. This variant (V109G) was correlated with post-operative calcitonin levels in the normal range and biochemical remission. Conversely, the wild-type (T/T) allele was associated with post-operative calcitonin levels above normal and a higher risk to develop clinical recurrence and distant metastases. Somatic RET mutations were significantly associated with a more aggressive behavior especially in wild-type allele-bearing patients.ConclusionsCollectively, in sporadic MTC, the CDKN1B V109G polymorphism correlates with a more favorable disease progression than the wild-type allele and might be considered a new promising prognostic marker.
Cross-Border Cooperation on Security in Europe
Authors: Bogacka Emilia 
Published: December 2010

Cross-Border Cooperation on Security in Europe
The aim of the article is to present cross-border cooperation on security. For this purpose, various problems in the European Union with respect to criminal policy must be described. The article consists of three parts. The first presents selected European institutions established to prevent and fight crime. The second concentrates on the control of external EU borders, quoting people's opinions on this matter and describing one of the EU programmes, the European Neighbourhood & Partnership Instrument: Cross-Border Cooperation. The third part focuses on security of the Polish borders as those which in recent years have witnessed serious political changes - Poland's accession to the European Union and the Schengen zone. The paper finishes with conclusions.
Interactions between Liquid Slag and Graphite During the Reduction of Metallic Oxides
Authors: Migas P. , Karbowniczek M. 
Published: December 2010

Interactions between Liquid Slag and Graphite During the Reduction of Metallic OxidesHigh global demand for metallic elements such as Fe, Cr, Ti, Mn leads to an intensive technological development of metal recovery. One of the ways is to recover metals from metallurgical slag. The sessile drop method was adopted in order to study the interactions between liquid slag and graphite. Steelmaking slag was used during this research. The slag used included converter slag and slag from chrome steel melting as well as their mixtures with other modifiers. The analysis covered six types of slag in the temperature ranging from 1100°C to 1600°C in the argon atmosphere. In the course of research mass spectrometry and scanning microscope were used. The following were set for the examined samples during the reduction process: liquidus temperature and the nature of interactions (wettability or its lack). It was observed that the volume of slag samples dynamically changed in the course of the reduction of metallic oxides.