bluuebunny/crossref_metadata_2025_split huggingface dataset:
Application of the Guanidine–Acylguanidine Bioisosteric Approach to Argininamide‐Type NPY Y2 Receptor Antagonists
Authors: Pluym Nikola , Brennauer Albert , Keller Max , Ziemek Ralf , Pop Nathalie , Bernhardt Günther , Buschauer Armin 
Published: June 2011

AbstractStrongly basic groups such as guanidine moieties are crucial structural elements, but they compromise the drug‐likeness of numerous biologically active compounds, including ligands of G‐protein‐coupled receptors (GPCRs). As part of a project focused on the search for guanidine bioisosteres, argininamide‐type neuropeptide Y (NPY) Y2 receptor (Y2R) antagonists related to BIIE0246 were synthesized. Starting from ornithine derivatives, NG‐acylated argininamides were obtained by guanidinylation with tailor‐made mono‐Boc‐protected N‐acyl‐S‐methylisothioureas. The compounds were investigated for Y2R antagonism (calcium assays), Y2R affinity, and NPY receptor subtype selectivity (flow cytometric binding assays). Most of the NG‐substituted (S)‐argininamides showed Y2R antagonistic activities and binding affinities similar to those of the parent compound, whereas NG‐acylated or ‐carbamoylated analogues with a terminal amine were superior (Y2R: Ki and KB values in the low nanomolar range). This demonstrates that the basicity of the compounds, although 4–5 orders of magnitude lower than that of guanidines, is sufficient to form key interactions with acidic amino acids of the Y2R. The acylguanidines bind with high affinity and selectivity to Y2R over the Y1, Y4, and Y5 receptors. As derivatization of the amino group is tolerated, these compounds can be considered building blocks for the preparation of versatile fluorescent and radiolabeled pharmacological tools for in vitro studies of the Y2R. The results support the concept of bioisosteric guanidine–acylguanidine exchange as a broadly applicable approach to retain pharmacological activity despite decreased basicity.
Triggering of TNFRSF25 promotes CD8+ T‐cell responses and anti‐tumor immunity
Authors: Slebioda Tomasz J. , Rowley Tania F. , Ferdinand John R. , Willoughby Jane E. , Buchan Sarah L. , Taraban Vadim Y. , Al‐Shamkhani Aymen 
Published: June 2011

AbstractTNFRSF25 is a member of the TNF receptor superfamily (TNFRSF) that binds to the TNF‐like protein TL1A. Although recent studies have demonstrated a role for TNFRSF25 in regulating CD4+ T‐cell responses, it remains to be determined if TNFRSF25 functions as a costimulatory receptor for CD8+ T cells. Here, we demonstrate that ectopic expression of TL1A on mouse plasmacytomas promotes elimination of tumor cells in a CD8+ T‐cell‐dependent manner and renders mice immune to a subsequent challenge with tumor cells. To gain further insight into the role of TNFRSF25 in CD8+ T‐cell responses, we analyzed the effect of TNFRSF25 triggering on OT‐I TCR transgenic T cells. We demonstrate that TNFRSF25 triggering in vivo with soluble TL1A promotes the proliferation and accumulation of antigen‐specific CD8+ T cells as well as their differentiation into CTLs. Furthermore, we show that TNFRSF25 also functions as a costimulatory receptor for memory CD8+ T cells. Thus, TNFRSF25 triggering enhances the secondary expansion of endogenous antigen‐specific memory CD8+ T cells. Our data suggest that TNFRSF25 agonists, such as soluble TL1A, could potentially be used to enhance the immunogenicity of vaccines that aim to elicit human anti‐tumor CD8+ T cells.
Vaccination with filamentous bacteriophages targeting DEC‐205 induces DC maturation and potent anti‐tumor T‐cell responses in the absence of adjuvants
Authors: Sartorius Rossella , Bettua Clotilde , D'Apice Luciana , Caivano Antonella , Trovato Maria , Russo Domenico , Zanoni Ivan , Granucci Francesca , Mascolo Dina , Barba Pasquale , Del Pozzo Giovanna , De Berardinis Piergiuseppe 
Published: June 2011

AbstractThe efficacy of a new vaccine‐delivery vector, based on the filamentous bacteriophage fd displaying a single‐chain antibody fragment known to bind the mouse DC surface molecule DEC‐205, is reported. We demonstrate both in vitro and in vivo an enhanced receptor‐mediated uptake of phage particles expressing the anti‐DEC‐205 fragment by DCs. We also report that DCs targeted by fd virions in the absence of other stimuli produce IFN‐α and IL‐6, and acquire a mature phenotype. Moreover, DC‐targeting with fd particles double‐displaying the anti‐DEC‐205 fragment on the pIII protein and the OVA257–264 antigenic determinant on the pVIII protein induced potent inhibition of the growth of the B16‐OVA tumor in vivo. This protection was much stronger than other immunization strategies and similar to that induced by adoptively transferred DCs. Since targeting DEC‐205 in the absence of DC activation/maturation agents has previously been described to result in tolerance, the ability of fd bacteriophages to induce a strong tumor‐specific immune response by targeting DCs through DEC‐205 is unexpected, and further validates the potential employment of this safe, versatile and inexpensive delivery system for vaccine formulation.
Neurophysiologic correlates of fMRI in human motor cortex
Authors: Hermes Dora , Miller Kai J. , Vansteensel Mariska J. , Aarnoutse Erik J. , Leijten Frans S.S. , Ramsey Nick F. 
Published: June 2011

AbstractThe neurophysiological underpinnings of functional magnetic resonance imaging (fMRI) are not well understood. To understand the relationship between the fMRI blood oxygen level dependent (BOLD) signal and neurophysiology across large areas of cortex, we compared task related BOLD change during simple finger movement to brain surface electric potentials measured on a similar spatial scale using electrocorticography (ECoG). We found that spectral power increases in high frequencies (65–95 Hz), which have been related to local neuronal activity, colocalized with spatially focal BOLD peaks on primary sensorimotor areas. Independent of high frequencies, decreases in low frequency rhythms (<30 Hz), thought to reflect an aspect of cortical‐subcortical interaction, colocalized with weaker BOLD signal increase. A spatial regression analysis showed that there was a direct correlation between the amplitude of the task induced BOLD change on different areas of primary sensorimotor cortex and the amplitude of the high frequency change. Low frequency change explained an additional, different part of the spatial BOLD variance. Together, these spectral power changes explained a significant 36% of the spatial variance in the BOLD signal change (R2 = 0.36). These results suggest that BOLD signal change is largely induced by two separate neurophysiological mechanisms, one being spatially focal neuronal processing and the other spatially distributed low frequency rhythms. Hum Brain Mapp, 2011. © 2011 Wiley‐Liss, Inc.
Working memory load modulation of parieto‐frontal connections: Evidence from dynamic causal modeling
Authors: Ma Liangsuo , Steinberg Joel L. , Hasan Khader M. , Narayana Ponnada A. , Kramer Larry A. , Moeller F. Gerard 
Published: June 2011

AbstractPrevious neuroimaging studies have shown that working memory load has marked effects on regional neural activation. However, the mechanism through which working memory load modulates brain connectivity is still unclear. In this study, this issue was addressed using dynamic causal modeling (DCM) based on functional magnetic resonance imaging (fMRI) data. Eighteen normal healthy subjects were scanned while they performed a working memory task with variable memory load, as parameterized by two levels of memory delay and three levels of digit load (number of digits presented in each visual stimulus). Eight regions of interest, i.e., bilateral middle frontal gyrus (MFG), anterior cingulate cortex (ACC), inferior frontal cortex (IFC), and posterior parietal cortex (PPC), were chosen for DCM analyses. Analysis of the behavioral data during the fMRI scan revealed that accuracy decreased as digit load increased. Bayesian inference on model structure indicated that a bilinear DCM in which memory delay was the driving input to bilateral PPC and in which digit load modulated several parieto‐frontal connections was the optimal model. Analysis of model parameters showed that higher digit load enhanced connection from L PPC to L IFC, and lower digit load inhibited connection from R PPC to L ACC. These findings suggest that working memory load modulates brain connectivity in a parieto‐frontal network, and may reflect altered neuronal processes, e.g., information processing or error monitoring, with the change in working memory load. Hum Brain Mapp, 2012. © 2011 Wiley Periodicals, Inc
Thyroglobulin measurement in fine‐needle aspiration biopsy of metastatic lymph nodes after rhTSH stimulation
Authors: Cappelli Carlo , Pirola Ilenia , De Martino Elvira , Gandossi Elena , Cimino Elena , Samoni Francesca , Agosti Barbara , Rosei Enrico Agabiti , Casella Claudio , Castellano Maurizio 
Published: June 2011

AbstractBackgroundThyroglobulin measurement in the washout of the needle used in fine‐needle aspiration cytology (FNAC) has been proposed for the early detection of lymph node metastasis both in patients with or without serum thyroglobulin antibodies; however, up to now, there have been no reports that recombinant human thyrotropin (rhTSH) stimulation modifies thyroglobulin measurement in lymph node aspirates.MethodsWe described, after rhTSH stimulation, the switching from undetectable to detectable levels of thyroglobulin in fine‐needle aspiration fluid from a suspected metastatic lymph node in 2 patients.ResultsWe hypothesized that thyroglobulin levels in the lymph node increased after rhTSH stimulation. The excess thyroglobulin saturates all thyroglobulin antibody binding sites and becomes detectable, explaining why it was undetectable when TSH was suppressed.ConclusionOn the basis of our experience, we suggest submitting to thyroglobulin measurement in the washout of the needle used in FNAC all patients with detectable serum thyroglobulin antibody after rhTSH stimulation. © 2011 Wiley Periodicals, Inc. Head Neck, 2013
A preliminary field test of an employee work passion model
Authors: Zigarmi Drea , Nimon Kim , Houson Dobie , Witt David , Diehl Jim 
Published: June 2011

AbstractFour dimensions of a process model for the formulation of employee work passion, derived from Zigarmi, Nimon, Houson, Witt, and Diehl (2009), were tested in a field setting. A total of 447 employees completed questionnaires that assessed the internal elements of the model in a corporate work environment. Data from the measurements of work affect, work cognition, job wellbeing, and work intention supported the hypothesized model. Structural equation modeling analyses indicated that a partial mediation model fitted best to the data. Implications for theory, practice, and future research directions are discussed.
A social‐contextual understanding of concordance and discordance between maternal prenatal representations of the infant and infant–mother attachment
Authors: Huth‐Bocks Alissa C. , Theran Sally A. , Levendosky Alytia A. , Bogat G. Anne 
Published: June 2011

AbstractThis prospective study examined the relationship between maternal prenatal representations of the infant and later infant–mother attachment, including contextual factors related to concordance and discordance among dyads over time. Participants were 173 pregnant women between the ages of 18 and 40 who were interviewed during their last trimester of pregnancy and 2 and 13 months after birth. Maternal representations were assessed by the Working Model of the Child Interview during pregnancy (WMCI; C.H. Zeanah, D. Benoit, L. Hirshberg, M.L. Barton, & C. Regan, 1994), and infant–mother attachment was assessed through the Strange Situation procedure (M.D.S. Ainsworth, M. Blehar, E. Waters, & S. Wall, 1978) when infants were 13 months old. There was substantial discordance between maternal and infant classifications, although a significant concordance rate was found when classifications were collapsed into balanced/secure and nonbalanced/insecure groups based on prenatal representations and postnatal infant attachment groups (60%; χ2 = 6.90, p < .01; κ .20). As expected, discordance between maternal representations and infant–mother attachment was meaningfully related to contextual risk factors, maternal depression, and infant behaviors.
Synthesis and Spectral Properties of Novel Poly(disubstituted acetylene)s
Authors: Duchoslavová Zuzana , Sivkova Radoslava , Hanková Vladimíra , Sedláček Jan , Svoboda Jan , Vohlídal Jiří , Zedník Jiří 
Published: June 2011

AbstractThe synthesis of mostly new acetylenes, R1CCR2, (R1 = 4‐t‐butylphenyl; R2 = 4‐t‐butylphenyl; 4‐[(t‐butyl)(diphenyl)silyloxy]phenyl; 1‐naphthyl; 2‐naphthyl; 9‐anthryl) is reported. Their UV‐vis characteristics are discussed in comparison to the results of TD‐DFT calculations. R1CCR2 (except for R2 = 9‐anthryl) give polyacetylenic polymers—[C(R1) = C(R2)]‐n, insoluble if R1 = R2 and well soluble if R1 ≠ R2 in polymerization with TaCl5/SnBu4. Polymerizability increases with increasing monomer triple bond accessibility for the catalyst. The photoluminescence yield of the soluble polymers rises in the R2 order: 1‐naphthyl < 2‐naphthyl < 4‐[(t‐butyl)(diphenyl)silyloxy]phenyl. magnified image
Older consumers' Tv home shopping: Loneliness, parasocial interaction, and perceived convenience
Authors: Lim Chae Mi , Kim Youn‐Kyung 
Published: June 2011

AbstractThis study examined factors that affect consumer satisfaction with TV shopping by investigating both convenience‐ and emotion‐related variables. Data were obtained from 295 online surveys with TV home shoppers who were 60 years and older. The structural model revealed (1) a positive effect of lack of shopping mobility on loneliness and perceived convenience of TV shopping, (2) a positive effect of loneliness on parasocial interaction, and (3) positive effects of parasocial interaction and perceived convenience on satisfaction with TV shopping. Results provide implications for TV shopping networks and producers. Parasocial interaction can be utilized to enhance the consumer experience by alleviating older consumers' loneliness, which ultimately leads to their satisfaction. Furthermore, convenience benefits of TV shopping can be emphasized to attract older consumers who have limited shopping mobility. © 2011 Wiley Periodicals, Inc.
Patterned Hydrophilization of Nanoporous 1,2‐PB by Thiol‐ene Photochemistry
Authors: Berthold Anton , Sagar Kaushal , Ndoni Sokol 
Published: June 2011

AbstractWe present an efficient method for functionalizing the large polymer–air interface of a gyroid nanoporous polymer. The hydrophilicity of nanoporous cross‐linked 1,2‐polybutadiene is tuned by thiol‐ene photo‐grafting of mercaptosuccinic acid or sodium 2‐mercaptoethanesulfonate. The reaction is monitored by FT‐IR, UV–Vis, contact angle, and gravimetry. Overall quantum yields are calculated for the two thiol‐ene “click” reactions in nano‐confinement, neatly revealing their chain‐like nature. Top–down photolithographic patterning is demonstrated, realizing hydrophilic nanoporous “corridors” exclusively hosting water. The presented approach can be relevant for many applications where, e.g., high control and contrast in hydrophilicity, chemical functionality or refractive index are needed. magnified image
Thermoreversibly Crosslinked Poly(ε‐caprolactone) as Recyclable Shape‐Memory Polymer Network
Authors: Defize Thomas , Riva Raphaël , Raquez Jean‐Marie , Dubois Philippe , Jérôme Christine , Alexandre Michaël 
Published: June 2011

AbstractA new concept to build shape memory polymers (SMP) combining outstanding fixity and recovery ratios (both above 99% after only one training cycle) typical of chemically crosslinked SMPs with reprocessability restricted to physically crosslinked SMPs is demonstrated by covalently bonding, through thermoreversible Diels–Alder (DA) adducts, star‐shaped poly(ε‐caprolactones) (PCL) end‐functionalized by furan and maleimide moieties. A PCL network is easily prepared by melt‐blending complementary end‐functional star polymers in retro DA regime, then by curing at lower temperature to favour the DA cycloaddition. Such covalent network can be reprocessed when heated again at the retro DA temperature. The resulting SMP shows still excellent shape memory properties attesting for its good recyclability.magnified image
Long‐Term management of DBS in dystonia: Response to stimulation, adverse events, battery changes, and special considerations
Authors: Tagliati Michele , Krack Paul , Volkmann Jens , Aziz Tipu , Krauss Joachim K. , Kupsch Andreas , Vidailhet and Marie 
Published: June 2011

AbstractMultiple independent case series have documented sustained benefit of bilateral pallidal deep brain stimulation (DBS) up to 3 years in patients with primary dystonia. Growing evidence exists for positive outcomes extending up to 10 years. The beneficial effects from DBS are usually reported to be stable, thus requiring little long‐term modifications of the parameters of stimulation. Speech and swallowing abnormalities are less responsive than other dystonic symptoms. Symptom exacerbation after initial benefit has been reported in a few cases. It is not known whether this is related to potential tolerance or habituation to stimulation or to progression of the underlying disease. Failures of pallidal DBS, at least in primary dystonia patients, should not be accepted without further re‐evaluation of each individual case, including possible revisions of the electrode location. Both hardware‐ and stimulation‐related adverse effects, including insufficient relief of speech function, have been reported in the long‐term. Despite early reports suggesting that hardware problems might be more frequent in dystonia, more recent studies did not confirm these observations. In patients with severe segmental (e.g., axial) or generalized dystonia, sudden cessation of stimulation may become a medical emergency and should be anticipated changing the neurostimulator before its natural end of life. © 2011 Movement Disorder Society
Early postoperative management of DBS in dystonia: Programming, response to stimulation, adverse events, medication changes, evaluations, and troubleshooting
Authors: Kupsch Andreas , Tagliati Michele , Vidailhet Marie , Aziz Tipu , Krack Paul , Moro Elena , Krauss Joachim K. 
Published: June 2011

AbstractEarly postoperative management in deep brain stimulation–treated patients with dystonia differs from that of patients with essential tremor and Parkinson's disease, mainly due to the usually delayed effects of deep brain stimulation and the heterogenous clinical manifestation and etiologies of dystonia. The present chapter summarizes the available data about and concentrates on practical clinical aspects of early postoperative management in deep brain stimulation–treated patients with dystonia. © 2011 Movement Disorder Society
Diet high in oat β‐glucan activates the gut‐hypothalamic (PYY3–36‐NPY) axis and increases satiety in diet‐induced obesity in mice
Authors: Huang Xu‐Feng , Yu Yinghua , Beck Eleanor J. , South Tim , Li Yulin , Batterham Marijka J. , Tapsell Linda C. , Chen Jiezhong 
Published: June 2011

AbstractThis study tested the effects of (1→3),(1→4) β‐D‐glucan from oats, on activation of the gut‐hypothalamic (PYY3–36‐NPY) axis, satiety, and weight loss in diet‐induced obesity (DIO) mice.DIO mice were fed standard lab chow diets or varied doses of β‐glucan for 6 weeks. Energy intake, satiety, body weight changes and peptide Y‐Y3‐36 (PYY3‐36) were measured together with a satiety test and measurement of neuropeptide Y (NPY) mRNA expression in the hypothalamic arcuate nucleus (Arc). The average energy intake (‐13%, p<0.05) and body weight gain was lower with increasing β‐glucan over 6 wk with acute suppression of energy intake over 4 h. The highest β‐glucan diet significantly increased plasma PYY3‐36, with suppression of Arc NPY mRNA.
Enrichment of peptides containing consensus sequence by an enzymatic approach for targeted analysis ofproteins
Authors: Wang Chunli , Ye Mingliang , Han Guanghui , Chen Rui , Zhang Manyu , Jiang Xinning , Cheng Kai , Wang Fangjun , Zou Hanfa 
Published: June 2011

AbstractMultiple residues with consensus sequence, i.e. motif, on proteins are closely related to protein function. However, there is no effective method for targeted analysis of such proteins. The challenge for analysis of these classes of proteins by MS is how to selectively enrich peptides containing consensus sequence from protein digest. Although enrichment of peptides containing one type of amino acid residue was successfully achieved by chemically labeling followed by chromatographic isolation, however, it is almost impossible to label and isolate signature peptides containing multiple residues with consensus sequence by chemical approach. Herein, we developed an enzymatic approach based on the specific recognition between enzyme and its substrates to enrich such peptides. This approach was realized by modification of a residue in the consensus sequence via enzyme that can recognize the sequence followed by the isolation of the modified peptides. cAMP‐dependent protein kinase was used to validate this approach and 168 peptides containing consensus motif were identified with selectivity of 67.2%. Those peptides resulted in the identification of 88 proteins with consensus sequence from serum sample. As this motif‐oriented peptide enrichment approach allows targeted analysis of a subset of proteins with consensus sequence, it will have broad application in biological studies.
Novel high‐performance polymer memory devices containing (OMe)2tetraphenyl‐p‐phenylenediamine moieties
Authors: CHen Chih‐Jung , Yen Hung‐Ju , Chen Wen‐Chang , Liou Guey‐Sheng 
Published: June 2011

AbstractThe functional polyimide (OMe)2TPPA‐6FPI (PI) and the polyamide (OMe)2TPPA‐6FPA (PA) consisting of electron‐donating N,N′‐bis(4‐aminophenyl)‐N,N′‐di(4‐methoxylphenyl)1,4‐phenylenediamine [(OMe)2TPPA‐diamine] for memory application were prepared in this study. These polyimide and polyamide memory devices were fabricated with the sandwich configuration of indium tin oxide (ITO)/polymer/Al, and could be switched from the initial low‐conductivity (OFF) state to the high‐conductivity (ON) state with high ON/OFF current ratios of 107 and 109, respectively. PI exhibited dynamic random access memory (DRAM) performance, whereas PA showed static random access memory (SRAM) behavior. To get more insight into the memory behaviors of these two different types of polymer memory devices, molecular simulation on the basic unit was carried out. Furthermore, the differences of highest occupied molecular orbital (HOMO) energy level, lowest unoccupied molecular orbital (LUMO) charge density isosurfaces, dipole moment, and linkage conformation between PI and PA were found to affect the volatile memory behavior. Both polymer memory devices revealed excellent stability with long operation time of 104 s at continuous applied voltage of ‐2 V. The effect of polymer thickness on the volatile memory behavior of PA was also investigated in this study. © 2011 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem, 2011