bluuebunny/crossref_metadata_2025_split huggingface dataset:
Family history of cancer and the risk of laryngeal cancer: A case‐control study from Italy and Switzerland
Authors: Garavello Werner , Turati Federica , Bosetti Cristina , Talamini Renato , Levi Fabio , Lucenteforte Ersilia , Chiesa Fausto , Franceschi Silvia , La Vecchia Carlo , Negri Eva 
Published: March 2011

AbstractOnly limited data is available on the relationship between family history of laryngeal and other neoplasms and laryngeal cancer risk. We investigated the issue using data from a multicentre case‐control study conducted in Italy and Switzerland between 1992 and 2009 including 852 cases with histologically confirmed laryngeal cancer and 1970 controls admitted to hospital for acute, non neoplastic conditions. Unconditional logistic regression models adjusted for age, sex, study center, education, tobacco smoking, alcohol drinking and number of siblings were used to estimate the odds ratios (ORs) of laryngeal cancer. The multivariate OR was 2.8 (95% confidence interval [CI], 1.5–5.3) in subjects reporting a first‐degree relative with laryngeal cancer, as compared to subjects with no family history. The OR was higher when the relative was diagnosed before 60 years of age (OR = 3.5, 95% CI 1.4–8.8). As compared to subjects without family history, non‐smokers, and moderate drinkers, the OR was 37.1 (95% CI 9.9–139.4) for current smokers, heavy drinkers, with family history of laryngeal cancer. Family history of colorectal (OR = 1.5, 95% CI 1.0–2.3) and kidney (OR = 3.8, 95% CI 1.2–12.1) cancer were also associated to an increased risk of laryngeal cancer, while no significant increase in risk was found for family history of cancer at all sites, excluding the larynx (OR = 1.1).
MET inhibition in tumor cells by PHA665752 impairs homologous recombination repair of DNA double strand breaks
Authors: Medová Michaela , Aebersold Daniel M , Zimmer Yitzhak 
Published: March 2011

AbstractAbnormal activation of cellular DNA repair pathways by deregulated signaling of receptor tyrosine kinase systems has broad implications for both cancer biology and treatment. Recent studies suggest a potential link between DNA repair and aberrant activation of the hepatocyte growth factor receptor Mesenchymal‐Epithelial Transition (MET), an oncogene that is overexpressed in numerous types of human tumors and considered a prime target in clinical oncology. Using the homologous recombination (HR) direct‐repeat direct‐repeat green fluorescent protein ((DR)‐GFP) system, we show that MET inhibition in tumor cells with deregulated MET activity by the small molecule PHA665752 significantly impairs in a dose‐dependent manner HR. Using cells that express MET‐mutated variants that respond differentially to PHA665752, we confirm that the observed HR inhibition is indeed MET‐dependent. Furthermore, our data also suggest that decline in HR‐dependent DNA repair activity is not a secondary effect due to cell cycle alterations caused by PHA665752. Mechanistically, we show that MET inhibition affects the formation of the RAD51‐BRCA2 complex, which is crucial for error‐free HR repair of double strand DNA lesions, presumably via downregulation and impaired translocation of RAD51 into the nucleus. Taken together, these findings assist to further support the role of MET in the cellular DNA damage response and highlight the potential future benefit of MET inhibitors for the sensitization of tumor cells to DNA damaging agents.
Brain‐derived neurotrophic factor is a potential osteoclast stimulating factor in multiple myeloma
Authors: Sun Chun‐Yan , Chu Zhang‐Bo , She Xiao‐Mei , Zhang Lu , Chen Lei , Ai Li‐Sha , Hu Yu 
Published: March 2011

AbstractMultiple myeloma (MM) is characterized by accumulation of monoclonal plasma cells in the bone marrow and progression of lytic bone lesions. The mechanisms of enhanced bone resorption in patients with myeloma are not fully defined. We have previously identified the role of brain‐derived neurotrophic factor (BDNF) in proliferation and migration of MM cells. In our study, we investigated whether BDNF was possibly involved in MM cell‐induced osteolysis. We showed that BDNF was elevated in MM patients and the bone marrow plasma levels of BDNF positively correlated with extent of bone disease. In osteoclast formation assay, bone marrow plasma from patients with MM increased osteoclast formation and the effect was significantly blocked by neutralizing antibody to BDNF, suggesting a critical role for BDNF in osteoclast activation. Furthermore, the direct effects of recombinant BDNF on osteoclast formation and bone resorption support the potential role of BDNF in the MM bone disease. BDNF receptor TrkB was expressed by human osteoclast precursors and a Trk inhibitor K252a markedly inhibited osteoclast formation stimulated with BDNF, demonstrating that BDNF used TrkB for its effects on osteoclast. Finally, bone marrow plasma BDNF level positively correlated with macrophage inflammatory protein‐1α and receptor activator of nuclear factor‐κB ligand, two major osteoclast stimulatory factors in MM. These results support an important role for BDNF in the development of myeloma bone disease.
Anomalous inhibition of c‐Met by the kinesin inhibitor aurintricarboxylic acid
Authors: Milanovic Mina , Radtke Simone , Peel Nick , Howell Michael , Carrière Virginie , Joffre Carine , Kermorgant Stéphanie , Parker Peter J. 
Published: March 2011

Abstractc‐Met [the hepatocyte growth factor (HGF) receptor] is a receptor tyrosine kinase playing a role in various biological events. Overexpression of the receptor has been observed in a number of cancers, correlating with increased metastatic tendency and poor prognosis. Additionally, activating mutations in c‐Met kinase domain have been reported in a subset of familial cancers causing resistance to treatment. Receptor trafficking, relying on the integrity of the microtubule network, plays an important role in activation of downstream targets and initiation of signalling events. Aurintricarboxylic acid (ATA) is a triphenylmethane derivative that has been reported to inhibit microtubule motor proteins kinesins. Additional reported properties of this inhibitor include inhibition of protein tyrosine phosphatases, nucleases and members of the Jak family. Here we demonstrate that ATA prevents HGF‐induced c‐Met phosphorylation, internalisation, subsequent receptor trafficking and degradation. In addition, ATA prevented HGF‐induced downstream signalling which also affected cellular function, as assayed by collective cell migration of A549 cells. Surprisingly, the inhibitory effect of ATA on HGF‐induced phosphorylation and signallingin vivowas associated with an increase in basal c‐Met kinase activityin vitro. It is concluded that the inhibitory effects of ATA on c‐Metin vivois an allosteric effect mediated through the kinase domain of the receptor. As the currently tested adenosine triphosphate competitive tyrosine kinase inhibitors (TKIs) may lead to tumor resistance (McDermott U, et al.,Cancer Res2010;70:1625–34), our findings suggest that novel anti‐c‐Met therapies could be developed in the future for cancer treatment.
Structure–function alteration of hemoglobin in arsenicosis patients: a probable pathway to exert toxicity
Authors: Mondal Bibaswan , Chatterjee Debdutta , Bhattacharyya Maitree 
Published: March 2011

ABSTRACTChronic arsenicosis, a major public health concern in India and Bangladesh, is mainly caused by ingestion of arsenic (As) contaminated ground water. Although this problem has been studied extensively, the mechanism of toxicity remains unknown. This paper investigates the process of trivalent arsenicals binding to hemoglobin (Hb) in chronic arsenicosis patients and consequent modification in the structure–function activity of Hb. In this work peroxidase activity, thermal denaturation profile, oxygen releasing capacity and hydrodynamic diameter have been evaluated for the Hb collected from subjects suffering with chronic arsenicosis. Increased peroxidative activity suggests altered oxidative status of Hb in the diseased state. The thermal denaturation profile indicates the Hb molecule to be more susceptible to unfolding in the pathologic state. The enhanced oxygen releasing capacity and significant reduction in hydrodynamic diameter of Hb is also observed in the diseased condition, suggesting conformational alterations in the Hb molecule. Finally, trivalent arsenic is found to bind with freshly isolated Hb from arsenicosis patients, binding affinity constant being 0.256 μM−1. The binding is positively cooperative with a Hill coefficient of +2.961 and isosbestic points at specific wavelengths. Thus, our work explores the structure–function property of Hb in chronic arsenicosis subjects and reveals that the molecule is modified in such a way that comparatively weak binding with oxygen and strong binding with arsenic occur simultaneously. This association may play a crucial role in exerting the pathway for arsenic toxicity. Copyright © 2011 John Wiley & Sons, Ltd.
Delayed response in the rat frontal lobe transcriptome to perinatal exposure to the flame retardant BDE‐47
Authors: Suvorov Alexander , Takser Larissa 
Published: March 2011

ABSTRACTBDE‐47 is the most prevalent congener of polybrominated diphenyl ethers, which are widely used flame retardants, and is known for endocrine and behavioral disrupting properties in animals. Transient effect on spontaneous motor activity in rats following perinatal exposure to BDE‐47 at low doses, relevant to human exposure, was reported in our previous study. The objective of this study was to screen for the long‐term effects on gene expression in the brain of rats perinatally exposed to BDE‐47. Wistar dams were exposed to BDE‐47 (0.002 and 0.2 mg kg−1 body weight) from gestation day 15 to postnatal day (PND) 20. Total RNA was extracted from the whole brain at PND10 and the brain frontal lobes at PND41 and hybridized to whole‐genome RNA expression microarrays. The genes, differentially expressed 1.5‐fold, were analyzed with the DAVID bioinformatics resources for cluster and gene‐term enrichment. At PND41, clusters of genes involved in nerve impulse transmission, nervous system development and functioning, and core biosynthetic process were altered, including several downregulated genes of cation channels. Representation of LINE1 RNA was decreased significantly. Altered expression of genes involved in neurodevelopment occured at least 3 weeks after the last exposure and the behavioral manifestation of low dose BDE‐47 toxicity. Copyright © 2011 John Wiley & Sons, Ltd.
Cell‐cycle phases and genetic profile of bone marrow‐derived mesenchymal stromal cells expanded in vitro from healthy donors
Authors: Achille Valentina , Mantelli Melissa , Arrigo Giulia , Novara Francesca , Avanzini Maria Antonietta , Bernardo Maria Ester , Zuffardi Orsetta , Barosi Giovanni , Zecca Marco , Maccario Rita 
Published: March 2011

AbstractHuman mesenchymal stromal cells (MSCs) expanded in vitro for cell therapy approaches need to be carefully investigated for genetic stability, by employing both molecular and conventional karyotyping. Reliability of cytogenetic analysis may be hampered in some MSC samples by the difficulty of obtaining an adequate number of metaphases. In an attempt to overcome this problem, a methodology apt to evaluate the cell‐cycle structure on synchronous MSCs was optimised. Results obtained in five independent experiments by comparing cell‐cycle analysis of synchronous and asynchronous MSC populations evaluated at early and late culture passages documented that in synchronous MSCs, 30% of cells entered G2/M phase after about 27–28 h of culture, while in asynchronous MSCs only 8% of cells in G2/M phase could be observed at the same time point. Cytogenetic analysis on synchronous MSCs allowed us to obtain 20–25 valuable metaphases/slide, whereas only 0–4 metaphases/slide were detectable in asynchronous preparations. J. Cell. Biochem. 112: 1817–1821, 2011. © 2011 Wiley‐Liss, Inc.
Removal of methylene blue from coloured effluents by adsorption onto SBA‐15
Authors: Dong Yanling , Lu Bin , Zang Shuying , Zhao Jingxiang , Wang Xiaoguang , Cai Qinghai 
Published: March 2011

AbstractAdsorption has been proven to be the most efficient method for quickly lowering the concentration of dissolved dyes in an effluent. In this regard, activated carbon is the most widely used adsorbent for removal of dyes from aqueous solution. However, the high cost of production and regeneration make it uneconomical. Therefore, inorganic adsorbents (e.g. zeolites) with high surface areas have been used as alternatives to carbon adsorbents. Microporous zeolites ZSM‐5, NH4‐Beta, MCM‐22 and mesoporous materials MCM‐41 have been investigated for the removal of dyes from aqueous solutions and they show effective adsorption performance. SBA‐15 possesses a larger pore size and pore wall thickness than MCM‐41. As a result, SBA‐15 has greater potential for the adsorption of methylene blue with larger molecule size and higher hydrothermal stability than the M41S family. SBA‐15 is an excellent adsorbent for methylene blue (MB), exhibiting 280 mg g−1 adsorption capacity and about 100% fading rate for MB. The adsorptive process is so fast that adsorption equilibrium is achieved in 5 min. In addition, SBA‐15 can be effectively recovered by calcination and reused 10 times without significant loss in removal of MB from aqueous solution. The efficient adsorption of MB molecules onto SBA‐15 was ascribed to MB adsorbed into the pore channels of SBA‐15, which was confirmed by nitrogen physisorption analysis of the adsorbent before and after adsorption. The long reuse life of the adsorbent suggests a high potential for application in industry. Copyright © 2010 Society of Chemical Industry
Yeast‐mediated stereo‐selective reduction of estrone by continuous cell culture with dual stirred tanks for product yield improvement
Authors: Cheng Cheanyeh , Tsai Hsiang‐Rong 
Published: March 2011

AbstractBACKGROUND: β‐Estradiol is an important hormone for the treatment of breast cancer and osteoporosis. β‐Estradiol can be produced via Saccharomyces cerevisiae mediated reduction of estrone. However, substrate inhibition and low production yield have been observed in the batch cell culture.RESULTS: An innovative continuous cell culture with dual stirred tanks in series was designed to solve the above problems. The growth medium was fed continuously to the incubation tank where the cells were incubated aerobically; the viable cells were then supplied continuously to the reaction tank in which the yeast‐mediated anaerobic reduction of estrone was performed with continuous feed of the substrate medium and continuous withdawal of the reaction cell culture. Thus, an increase in cell productivity from about 3‐fold to 7‐fold was obtained when compared with the batch cell culture. The β‐estradiol yield was improved to 64.8% on the second reaction day, accompanied by an accumulation of 12.9 mg β‐estradiol on the third reaction day. The yield was about 10% more and the accumulated recovery of β‐estradiol was 4.3‐fold better than with the batch cell culture. The diastereomeric excess value (%de) of β‐estradiol was more than 99%.CONCLUSION: A high product yield with excellent stereo‐selectivity was achieved in a short reaction period with the developed continuous cell culture and the dual stirred tank. Copyright © 2010 Society of Chemical Industry
Article alerts: Items from 2009, Part II
Authors: Hafdahl Adam R. 
Published: March 2011

AbstractThis second installment of the ‘Article Alerts’ feature section more than doubles the collection's number of items. The print and archive versions now include 200 and 2579 items, respectively. Remarks are offered on inclusion criteria, anticipated growth of the archive version, and a related resource. Copyright 2011 John Wiley & Sons, Ltd.
‘Cross hairs’ plots for diagnostic meta‐analysis
Authors: Phillips Bob , Stewart Lesley A. , Sutton Alex J. 
Published: March 2011

AbstractUnderstanding diagnostic test accuracy (DTA) data, especially meta‐analysis of this information, can be challenging for researchers and clinicians. The use of plots of receiver‐operator curve (ROC) space showing individual studies and summary estimates of diagnostic accuracy has become common but can be difficult to interpret. The assessment of heterogeneity in sensitivity and specificity across studies can be particularly difficult. In this paper, we review the key concepts of assessing DTA, starting at the level of individual studies and progressing to the setting of research synthesis. We explore the standard displays of this information and then propose and explain an alternative approach to summarizing key data. These ‘cross‐hairs’ plots display the individual studies in ROC space with paired confidence intervals representing sensitivity and specificity, and allow for the results of meta‐analysis to be overlaid on the plot. We suggest that these plots are more easily interpreted, and are a more informative graphical form than common approaches. Copyright © 2011 John Wiley & Sons, Ltd.
The total number of resected lymph node is not a prognostic factor for recurrence in esophageal squamous cell carcinoma patients undergone transthoracic esophagectomy
Authors: Hsu Po‐Kuei , Wang Bing‐Yen , Chou Teh‐Ying , Huang Chien‐Sheng , Wu Yu‐Chung , Hsu Wen‐Hu 
Published: March 2011

AbstractBackgroundThe total number of resected lymph nodes (TLN) has been shown to predict survival in esophageal cancer, but its relationship with recurrence has been rarely reported. We aim to study the prognostic factors in esophageal squamous cell carcinoma (ESCC) patients, with a particular focus on the role of TLN.MethodsTwo hundred sixty‐eight ESCC patients who underwent transthoracic esophagectomy were selected for the study. A Cox regression model was used to identify prognostic factors.ResultsRecurrence occurred in 115 of 268 patients. The median time to recurrence was 10 months (range, 1–58). The recurrence‐free survival at 1, 3, and 5 years was 62.3%, 32.1%, and 28.5%, respectively. Multivariate analysis identified age (P = 0.001), N stage (N1–3 vs. N0, P = 0.001), tumor length (P = 0.019), and development of recurrence (P < 0.001) as independent prognostic factors for overall survival, whereas T (T3/4 vs. T1/2, P = 0.029) and N stage (N1–3 vs. N0, P = 0.017) were independent prognostic factors for recurrence. TLN was a significant factor only when predicting overall survival in N0 patients (HR, 0.976; 95% CI, 0.953–0.999; P = 0.042).ConclusionThe TLN is not a prognostic factor for recurrence in ESCC patients undergone transthoracic esophagectomy. J. Surg. Oncol. 2011; 103:416–420. © 2011 Wiley‐Liss, Inc.
Prognostic analysis of familial gastric cancer in Chinese population
Authors: Ye Yan‐Wei , Dong Rui‐Zheng , Zhou Ye , Du Chun‐Yan , Wang Chun‐Meng , Fu Hong , Shi Ying‐Qiang 
Published: March 2011

AbstractBackgroundThe aim of this study was firstly to elucidate the prognosis of familial gastric cancer (FGC) in Chinese population.MethodsA total of 162 patients were recruited, including 81 patients with FGC and 81 patients with sporadic gastric cancer (SGC), who underwent gastrectomy between 1996 and 2007. Paraffin‐embedded tumor specimens were obtained from tissue bank of Cancer Hospital, Fudan University. The expression of epidermal growth factor receptor (EGFR), P53, C‐myc, and proliferating cell nuclear antigen (PCNA) were detected by immunohistochemical method.ResultsThere were significant differences in tumor size, vessel invasion, EGFR, and P53 expression between FGC and SGC patients. The 5‐year survival rates were 48% and 57% in FGC and SGC patients, respectively (P = 0.033). Subgroup analysis showed that the 5‐year survival rates were worse in FGC patients with nerve invasion, high PCNA expression, negative expression of EGFR, and positive expression of P53 than those in SGC group. Multivariate analysis showed that AJCC stage, tumor size, and nerve invasion were independent prognostic factors in all patients. Furthermore, AJCC stage and P53 expression dramatically affected the prognosis of FGC patients.ConclusionsThe prognosis of FGC patients might be worse than those of SGC patients. AJCC stage and P53 expression are independent prognostic factors in FGC patients.J. Surg. Oncol. 2011;104:76–82. © 2011 Wiley‐Liss, Inc.
A reconstruction with extracorporeal irradiated autograft in osteosarcoma around the knee
Authors: Kim Jae‐Do , Lee Gun Woo , Chung So Hak 
Published: March 2011

AbstractBackground and ObjectivesAn extracorporeal irradiated (ECI) autograft was first reported in Israel in 1968 for the treatment of primary malignant bone tumor. However, subsequent reports covered mostly short‐term follow up results, and the reported results of reconstruction vary from study to study. We analyze the long‐term follow up results of reconstruction using an extracorporeal irradiated (ECI) autograft in osteosarcoma around the knee.MethodsWe retrospectively reviewed 23 osteosarcoma patients who underwent reconstruction with ECI between December 1995 and April 2005. The 15 males and 8 females had a mean age of 21 years (range: 7–74) and a mean follow‐up of 127 months(range: 57–170). Tumors were located in the distal femur in 14 cases, and the proximal tibia in 9 cases. Pathological types were conventional in 22 cases, and parosteal in 1 case. Resection methods were osteoarticular in 18 cases and total joint in 5 cases. Eighteen cases were reconstructed with the ECI autograft and five with ECI autograft‐prosthesis composite (APC). The Musculoskeletal Tumor Society (MSTS) score was used for functional evaluation. The overall survival rate, local recurrence, complications were analyzed.ResultsThe overall survival rate was 82.6%, and the disease‐free survival rate was 69.5%. We found 20 complications, including 6 nonunions, 5 deep infections, 4 joint instabilities, 2 fractures, 1 limb‐length discrepancy (LLD), 1 epiphyseal collapse, and 1 vessel occlusion. The average MSTS functional score was 62.6%.ConclusionsReconstruction with an extracorporeal irradiated is not recommended for osteosarcoma around the knee. J. Surg. Oncol. 2011;104:187–191. © 2011 Wiley‐Liss, Inc.
Monolith as a new sample preparation material: Recent devices and applications
Authors: Namera Akira , Nakamoto Akihiro , Saito Takeshi , Miyazaki Shota 
Published: March 2011

AbstractMonolith was first used as a material for chromatographic separation two decades ago and solid‐phase extraction over 10 years, and since then, separation science has undergone a dramatic change owing to advancements in analytical technology. Recently, monolith has been modified to suit various devices for the extraction and enrichment of analytes in any matrices of environmental, food, and biological analyses. This approach has contributed to miniaturization and automation for sample preparation, and it can reduce the time and cost requirements of sample preparation. Recently, numerous applications have been demonstrated for online and inline preconcentration coupled with monolith, and many kinds of devices have been designed and developed for offline devices. In this review, these applications and devices are listed and discussed in reference to other fields.